bluuebunny/crossref_metadata_2025_split huggingface dataset:
Publications by Scandinavian university Departments of Anaesthesiology from 2001 to 2015
Authors: Miller C. , Prenn R. , Ausserer J. , Hamm P. , Neururer S. , Paal P. 
Published: May 2018

BackgroundThis study assessed the contribution made to research by Scandinavian university Departments of Anaesthesiology and their distribution among those departments.MethodsWe committed a PubMed search for all publications originating from Scandinavian university Departments of Anaesthesiology between 2001 and 2015. Articles were assigned to their author's affiliated university department and to 3 time periods 2001‐2005, 2006‐2010 and 2011‐2015. The number of publications, original articles and citations were analysed.ResultsThe 23 Scandinavian university Departments of Anaesthesiology published 5190 articles between 2001 and 2015, which make up 91.5% from all anaesthesiology research originating from Scandinavia. Of these 1760 (33.9%) were classified as original articles. The number of publications increased in the observed periods (from 1305 in 2001‐2005 to 2452 in 2010‐2015), but the share of original articles decreased (from 40% to 31%). Finnish university Departments of Anaesthesiology had the largest share of original articles. The university department of Copenhagen was the most productive Department of Anaesthesiology in terms of number of publications (n = 1187) and citation rate of original articles (median 24 citations per original article). Publications from Bergen scored the highest impact (median 19 citations per publication).ConclusionsThe number of publications from Scandinavian university Departments of Anaesthesiology increased between 2001 and 2015, but the share of original articles decreased. Copenhagen was the most productive university Department of Anaesthesiology based on the number of publications and citations of original articles. Finnish university Departments of Anaesthesiology led in the share of original articles out of all publications.
Out‐of‐hospital cardiac arrests in nursing homes and primary care facilities in Pirkanmaa, Finland
Authors: Kangasniemi H. , Setälä P. , Huhtala H. , Kämäräinen A. , Virkkunen I. , Jämsen E. , Yli‐Hankala A. , Hoppu S. 
Published: May 2018

BackgroundDispatching Emergency Medical Services to treat patients with deteriorating health in nursing homes and primary care facilities is common in Finland. We examined the cardiac arrest patients to describe this phenomenon. We had a special interest in patients for whom cardiopulmonary resuscitation was considered futile.MethodsWe conducted an observational study between 1 June 2013 and 31 May 2014 in the Pirkanmaa area. We included cases in which Emergency Medical Services participated in the treatment of cardiac arrest patients in nursing homes and primary care facilities.ResultsEmergency Medical Services attended to a total of 355 cardiac arrest patients, and 65 patients (18%) met the inclusion criteria. The included patients were generally older than 65 years, but otherwise heterogeneous. Nineteen patients (29%) had a valid do‐not‐attempt‐resuscitation order, but paramedics were not informed about it in 10 (53%) of those cases. Eight (12%) of the 65 patients survived to hospital admission and 3 (5%) survived to hospital discharge with a neurologically favourable outcome. Two patients were alive 90 days after the cardiac arrest; both were younger than 70 years of age and had ventricular fibrillation as primary rhythm. There were no survivors in nursing homes.ConclusionsThe do‐not‐attempt‐resuscitation orders were often unavailable during a cardiopulmonary resuscitation attempt. Although resuscitation attempts were futile for patients in nursing homes, some patients in primary care facilities demonstrated a favourable outcome after cardiac arrest. Emergency Medical Services seem to be able to recognise potential survivors and focus resources on their treatment.
Advanced glycation end products and their receptors did not show any association with body mass parameters in metabolically healthy adolescents
Authors: Garay‐Sevilla Ma. Eugenia , Torres‐Graciano Sofía , Villegas‐Rodríguez Ma. Etzabel , Rivera‐Cisneros Antonio E. , Wrobel Katarzyna , Uribarri Jaime 
Published: May 2018

AbstractAimWe determined the relationship between circulating advanced glycation end products (AGEs), AGE receptors and homeostatic model assessment for insulin resistance (HOMA‐IR) in metabolically healthy obese and normal weight adolescents.MethodsIn 2015, we recruited 80 normal weight adolescents and 80 with obesity from schools Leon city, Mexico, and put them into metabolically healthy (HOMA‐IR <3.0) and unhealthy (HOMA‐IR >3.0) groups. We measured their body mass index (BMI) and carried out detailed blood analyses.ResultsWe found a higher triglycerides, triglycerides/high‐density lipoproteins cholesterol (TG/HDL‐C) index, HOMA‐IR, tumour necrosis factor alpha (TNF‐α) and interleukin‐6 (IL‐6) in the metabolically healthy group and found correlations between HOMA‐IR with BMI, the TG/HDL‐C index and IL‐6 and the TG/HDL‐C index and BMI and (TNF‐α). There was no correlation between markers of obesity and circulating N‐carboxymethyl‐lysine (CML) or soluble receptor for advanced glycation end products (sRAGE). Some unhealthy adolescents had higher CML (15.5 ± 2.7 U/mL, p < 0.028) and sRAGE (3123 ± 1364 pg/mL, p < 0.001) than the healthy group.ConclusionHOMA‐IR and the TG/HDL‐C index were associated with BMI and inflammation markers. CML and sRAGE were not associated with obesity or inflammation. These parameters were higher in unhealthy obese adolescents.
Effects of inoculants and environmental temperature on fermentation quality and bacterial diversity of alfalfa silage
Authors: Zhang Qing , Yu Zhu , Wang Xianguo , Tian Jipeng 
Published: May 2018

AbstractTo investigate the effects of inoculants and environmental temperature on fermentation quality and bacterial diversity of alfalfa silage, first‐cut alfalfa was ensiled with or without two screened lactic acid bacteria (LAB) strains, Lactobacillus plantarum, LP, and Lactobacillus casei, LC. Each treatment was divided into three parts and stored at 20°C, 30°C, 40°C, respectively. After 60 days ensiling, fermentation characteristics were measured and bacterial diversity was investigated by 16S ribosomal RNA gene sequencing using Illumina MiSeq platform. LP and LC decreased pH, coliform bacteria counts and increased lactic acid content at 20°C, and the two strains decreased pH, ammonia‐N concentration, coliform bacteria counts at 30°C. When the environmental temperature was 40°C, silage treated with LC showed lower LAB and coliform bacteria counts and higher lactic acid content than the untreated and LP treated silages. Butyric acid mainly appeared in silages stored at 40°C. The relative abundance of Lactobacillus in alfalfa silages stored at 20°C and 30°C was highest and increased after LP and LC were added. Garciella was another dominant genus in silages stored at 40°C. In conclusion, LP and LC improved fermentation quality of alfalfa silage by increasing Lactobacillus proportions at 20°C and 30°C; ensiling alfalfa at 40°C was difficult because of Garciella.
Family‐centered kindergarten social work services in Hong Kong: A pilot project
Authors: Lau Yuk King , Ho Wai Ming 
Published: May 2018

AbstractSocial work services at schools in Hong Kong will begin a new chapter in kindergartens in 2018–2019 school year. Early childhood professionals have long considered family‐centered service delivery best practice. This paper reports a pioneer project on a family‐centered approach in kindergarten social work services in Hong Kong. The project was illustrated according to the following family‐centered principles: (a) The family as the unit of attention, (b) Linking families with community resources, (c) Flexible and individualized service provision, and (d) Creating partnerships and supportive relationships. The value and contributions of family‐centered social work services in kindergartens and implications for social work training in Hong Kong are discussed.
Generation of a multi‐functional, target organ‐specific, anti‐fibrotic molecule by molecular engineering of the extracellular matrix protein, decorin
Authors: Järvinen Tero A H , Ruoslahti Erkki 
Published: May 2018

AbstractExtracellular matrix (ECM) molecules play important roles in regulating processes such as cell proliferation, migration, differentiation and survival. Decorin is a proteoglycan that binds to (‘decorates’) collagen fibrils in the ECM. Decorin also interacts with many growth factors and their receptors, the most notable of these interactions being its inhibitory activity on TGF‐β, the growth factor responsible for fibrosis formation. We have generated a recombinant, multi‐functional, fusion‐protein consisting of decorin as a therapeutic domain and a vascular homing and cell‐penetrating peptide as a targeting vehicle. This recombinant decorin (CAR‐DCN) accumulates at the sites of the targeted disease at higher levels and, as a result, has substantially enhanced biological activity over native decorin. CAR‐DCN is an example of how molecular engineering can give a compound the ability to seek out sites of disease and enhance its therapeutic potential. CAR‐DCN will hopefully be used to treat severe human diseases.Linked ArticlesThis article is part of a themed section on Translating the Matrix. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v176.1/issuetoc
The phosphodiesterase 5 inhibitor, KJH‐1002, reverses a mouse model of amnesia by activating a cGMP/cAMP response element binding protein pathway and decreasing oxidative damage
Authors: Zhang Lijun , Seo Jae Hong , Li Huan , Nam Ghilsoo , Yang Hyun Ok 
Published: May 2018

Background and PurposeInhibition of PDE5 improves synaptic plasticity and memory via enhancing cGMP expression, thus activating the cGMP/cAMP response element binding protein (CREB) signalling pathway. This study investigated the effects of a PDE5 inhibitor on scopolamine‐induced cognitive dysfunction, using memory‐related behavioural tests and biochemical assays.Experimental ApproachIn mice were pretreated with PDE5 inhibitor, amnesia was induced by scopolamine. The learning and memory abilities of mice were tested using the Morris water maze test, the Y‐maze test, the passive avoidance test and the novel object recognition test in sequence. Expression of memory‐related bio‐molecules and oxidative stress parameters in brain tissue were measured using Western blot and spectrophotometry respectively.Key ResultsKJH‐1002, a novel and potent inhibitor of PDE5 (IC50 0.059 ± 0.04 nmol·L−1), was synthesized. In the behavioural tests, it markedly improved the memory performance impaired by scopolamine, indicating a restoration of cognitive function in the mice. Moreover, KJH‐1002 increased cGMP levels in the cortex and the scopolamine‐reduced expression of phosphorylated CREB, Levels of ERK 1/2, Akt and brain‐derived neurotrophic factor in the cortex and hippocampus were restored by KJH‐1002 treatment. In addition, KJH‐1002 administration increased the activities of SOD, glutathione peroxidase and glutathione reductase, and decreased the level of malondialdehyde.Conclusion and ImplicationsKJH‐1002 restored cognitive function in scopolamine‐induced amnesia mice by activating the cGMP/CREB signalling pathway and attenuating oxidative stress. The beneficial effects of KJH‐1002 on cognition indicate its potential as a therapeutic candidate for Alzheimer's disease.
Methylisothiazolinone contact allergy in Croatia: Epidemiology and course of disease following patch testing
Authors: Ljubojević Hadžavdić Suzana , Uter Wolfgang , Ilijanić Samošćanec Maja , Johansen Jeanne D. 
Published: May 2018

BackgroundMethylisothiazolinone (MI) caused an epidemic of contact allergy in Europe, as shown by data from many countries, but no studies from Croatia exist. Also, data are lacking on the severity of allergic contact dermatitis (ACD) caused by MI, and its impact on quality of life and prognosis.ObjectivesTo determine the frequency of MI contact allergy among Croatian dermatitis patients, identify causative exposures, assess the impact of disease, and study the prognosis.MethodsData were collected for consecutive dermatitis patients with MI contact allergy patch tested in Croatia between November 2, 2015 and November 3, 2016.ResultsMI contact allergy was diagnosed in 13.2% of 798 tested patients. The most frequent dermatitis locations were the hands (76%) and face (61%). In 89.3% of patients, MI contact allergy was found to be of current relevance. Considerable severity and impact on daily life of disease was found at the first consultation, but this significantly decreased until follow‐up 3 months later.ConclusionsPatch testing is the standard method for the diagnosis of ACD, and it has been shown to have an important beneficial effect on prognosis. The severity of MI ACD and the impact on daily life emphasize the need for prevention.
Diversity of pathogenic and endophytic Colletotrichum isolates from Licania tomentosa in Brazil
Authors: Lisboa Daniela O. , Silva Mariana A. , Pinho Danilo B. , Pereira Olinto L. , Furtado Gleiber Q. 
Published: May 2018

AbstractLicania tomentosa (Chrysobalanaceae), also known as “oiti,” is a forest tree mainly used for urban afforestation in Brazil. Although anthracnose caused by Colletotrichum gloeosporioides is the main disease that threatens this tree, the identification of the species was based on morphological characteristics only. Owing to the need to use the molecular approach to pinpoint the identity of this pathogen with precision, the aim of this study was to identify endophytic and pathogenic Colletotrichum isolates from L. tomentosa based on both morphological and molecular data. For prior identification, partial sequences of the GAPDH region were obtained of all the 35 isolates (10 endophytic and 25 pathogenic). After analysis, ten isolates, representative of each clade, were selected for multilocus phylogenetic analysis (ACT, CAL, CHS‐1, GAPDH, TUB2, SOD2 and ITS). In addition, a tree based on the ApMat region was obtained for comparison with the multilocus tree. Morphological characterization (colony growth, conidial size and appressoria shape) was also performed for each species. To prove pathogenicity, L. tomentosa leaves were inoculated on the adaxial surface by mycelial plugs and conidial suspension. All isolates obtained belong to the Colletotrichum gloeosporioides complex. The Apmat tree has the same topology as the multilocus tree, allowing for the discrimination of the different species of Colletotrichum on L. tomentosa. Endophytic isolates of C. fructicola, C. queenslandicum, and C. siamense were acquired whereas pathogenic isolates were identified as C. siamense and C. tropicale, although all species were pathogenic on the wounded leaves of L. tomentosa. This is the first worldwide report of this Colletotrichum species associated with L. tomentosa and the first recording of C. queenslandicum in Brazil.
Lasting changes induced by mild alcohol exposure during embryonic development in BDNF, NCAM and synaptophysin‐positive neurons quantified in adult zebrafish
Authors: Mahabir Samantha , Chatterjee Dipashree , Misquitta Keith , Chatterjee Diptendu , Gerlai Robert 
Published: May 2018

AbstractFetal alcohol spectrum disorder is one of the leading causes of mental health issues worldwide. Analysis of zebrafish exposed to alcohol during embryonic development confirmed that even low concentrations of alcohol for a short period of time may have lasting behavioral consequences at the adult or old age. The mechanism of this alteration has not been studied. Here, we immersed zebrafish embryos into 1% alcohol solution (vol/vol%) at 24 hr post‐fertilization (hpf) for 2 hr and analyzed potential changes using immunohistochemistry. We measured the number of BDNF (brain‐derived neurotrophic factor) and NCAM (neuronal cell adhesion molecule)‐positive neurons and the intensity of synaptophysin staining in eight brain regions: lateral zone of the dorsal telencephalic area, medial zone of the dorsal telencephalic area, dorsal nucleus of the ventral telencephalic area, ventral nucleus of the ventral telencephalic area, parvocellular preoptic nucleus, ventral habenular nucleus, corpus cerebella and inferior reticular formation. We found embryonic alcohol exposure to significantly reduce the number of BDNF‐ and NCAM‐positive cells in all brain areas studied as compared to control. We also found alcohol to significantly reduce the intensity of synaptophysin staining in all brain areas except the cerebellum and preoptic area. These neuroanatomical changes correlated with previously demonstrated reduction of social behavior in embryonic alcohol‐exposed zebrafish, raising the possibility of a causal link. Given the evolutionary conservation across fish and mammals, we emphasize the implication of our current study for human health: even small amount of alcohol consumption may be unsafe during pregnancy.
Protein kinases involved in epidermal barrier formation: The AKT family and other animals
Authors: Rogerson Clare , O'Shaughnessy Ryan F. L. 
Published: May 2018

AbstractFormation of a stratified epidermis is required for the performance of the essential functions of the skin; to act as an outside‐in barrier against the access of microorganisms and other external factors, to prevent loss of water and solutes via inside‐out barrier functions and to withstand mechanical stresses. Epidermal barrier function is initiated during embryonic development and is then maintained throughout life and restored after injury. A variety of interrelated processes are required for the formation of a stratified epidermis, and how these processes are both temporally and spatially regulated has long been an aspect of dermatological research. In this review, we describe the roles of multiple protein kinases in the regulation of processes required for epidermal barrier formation.
Tundra be dammed: Beaver colonization of the Arctic
Authors: Tape Ken D. , Jones Benjamin M. , Arp Christopher D. , Nitze Ingmar , Grosse Guido 
Published: May 2018

AbstractIncreasing air temperatures are changing the arctic tundra biome. Permafrost is thawing, snow duration is decreasing, shrub vegetation is proliferating, and boreal wildlife is encroaching. Here we present evidence of the recent range expansion of North American beaver (Castor canadensis) into the Arctic, and consider how this ecosystem engineer might reshape the landscape, biodiversity, and ecosystem processes. We developed a remote sensing approach that maps formation and disappearance of ponds associated with beaver activity. Since 1999, 56 new beaver pond complexes were identified, indicating that beavers are colonizing a predominantly tundra region (18,293 km2) of northwest Alaska. It is unclear how improved tundra stream habitat, population rebound following overtrapping for furs, or other factors are contributing to beaver range expansion. We discuss rates and likely routes of tundra beaver colonization, as well as effects on permafrost, stream ice regimes, and freshwater and riparian habitat. Beaver ponds and associated hydrologic changes are thawing permafrost. Pond formation increases winter water temperatures in the pond and downstream, likely creating new and more varied aquatic habitat, but specific biological implications are unknown. Beavers create dynamic wetlands and are agents of disturbance that may enhance ecosystem responses to warming in the Arctic.
Nuclear–cytoplasmic shuttling protein PP2AB56 contributes to mTORC1‐dependent dephosphorylation of FOXK1
Authors: Nakatsumi Hirokazu , Oka Takeru , Higa Tsunaki , Shirane Michiko , Nakayama Keiichi I. 
Published: May 2018

AbstractMammalian target of rapamycin complex 1 (mTORC1) kinase is a master regulator of the cellular response to nutrition‐related signals such as insulin and amino acids. mTORC1 is activated on the lysosomal membrane and induces phosphorylation of a variety of downstream molecules. We previously showed that activated mTORC1 induces protein phosphatase 2A (PP2A)‐mediated dephosphorylation of the transcription factor forkhead box K1 (FOXK1). The mechanism underlying the signal transduction from the cytoplasmic mTORC1 to the nuclear FOXK1 has remained unclear, however, we now show that a nuclear–cytoplasmic transport system is necessary for the mTORC1‐FOXK1 signal transduction. This reaction is mediated by a shuttling protein B56, which is a regulatory subunit of PP2A and plays an essential role in the mTORC1‐dependent dephosphorylation of FOXK1. These results suggest that PP2AB56 phosphatase contributes to the signaling for mTORC1‐dependent transcriptional regulation.
Nutrient conditions sensed by the reproductive organ during development optimize male fecundity in Drosophila
Authors: Kubo Ayuko , Matsuka Mirai , Minami Ryunosuke , Kimura Fumika , Sakata‐Niitsu Rumi , Kokuryo Akihiko , Taniguchi Kiichiro , Adachi‐Yamada Takashi , Nakagoshi Hideki 
Published: May 2018

AbstractNutrient conditions affect the reproductive potential and lifespan of many organisms through the insulin signaling pathway. Although this is well characterized in female oogenesis, nutrient‐dependent regulation of fertility/fecundity in males is not known. Seminal fluid components synthesized in the accessory gland are required for high fecundity in Drosophila males. The accessory gland is composed of two types of binucleated cells: a main cell and a secondary cell (SC). The transcription factors Defective proventriculus (Dve) and Abdominal‐B (Abd‐B) are strongly expressed in adult SCs, whose functions are essential for male fecundity. We found that gene expression of both Dve and Abd‐B was down‐regulated under nutrient‐poor conditions. In addition, nutrient conditions during the pupal stage affected the size and number of SCs. These morphological changes clearly correlated with fecundity, suggesting that SCs act as nutrient sensors. Here, we provide evidence that Dve associates nutrient conditions with optimal reproductive potential in a target of rapamycin signaling‐dependent manner.