bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Dutch Are Divided
Authors: Schabas William A. 
Published: November 2018

Abstract
The Kaiser’s legal status is initially uncertain because he did not formally abdicate his thrones until the end of November. As a neutral state, the Netherlands was required to detain foreign combatants. The Netherlands had many pro-German elements, including the Royal Court and the Foreign Minister. But many in the country did not welcome his arrival. The government denied charges that the Kaiser was invited into the country by the Queen. There were fierce debates in Parliament and in the press about whether he should be allowed to remain in the country. Dutch experts set out the legal framework of the Kaiser’s presence in the country.
Multinational Banks and Supranational Supervision
Authors: Calzolari Giacomo , Colliard Jean-Edouard , Lóránth Gyongyi 
Published: November 2018

Abstract
Supervision of multinational banks (MNBs) by national supervisors suffers from coordination failures. We show that supranational supervision solves this problem and decreases the public costs of an MNB’s failure, taking its organizational structure as given. However, the MNB strategically adjusts its structure to supranational supervision. It converts its subsidiary into a branch (or vice versa) to reduce supervisory monitoring. We identify the cases in which this endogenous reaction leads to unintended consequences, such as higher public costs and lower welfare. Current reforms should consider that MNBs adapt their organizational structures to changes in supervision.
Received January 9, 2017; editorial decision September 15, 2018 by Editor Philip Strahan. Authors have furnished an Internet Appendix, which is available on the Oxford University Press Web site next to the link to the final published paper online.
Encouraging patients to disclose their lesbian, gay, bisexual, or transgender (LGBT) status: oncology health care providers’ perspectives
Authors: Banerjee Smita C , Staley Jessica M , Alexander Koshy , Walters Chasity B , Parker Patricia A 
Published: November 2018

Abstract
A compelling touted strategy for reducing discrimination towards lesbian, gay, bisexual, and transgender (LGBT) patients is improving communicative competence of health care providers (HCPs); however, evidence base for describing communication practices between HCPs and LGBT patients is scarce. The purpose of this study was to qualitatively examine HCP experiences and perspectives as they relate to patient sexual orientation and gender identity (SOGI) disclosure, perceived communication and structural/administrative challenges in interactions with LGBT patients, and suggestions for improving care of LGBT patients. The sample consisted of 1,253 HCPs, who provided open-ended responses to an online cross-sectional survey conducted at a Comprehensive Cancer Center in the Northeastern United States. The open-ended responses were inductively and deductively coded for key themes and sub-themes. The results demonstrated an array of useful communication strategies employed by oncology HCPs to encourage LGBT patients’ SOGI disclosure (direct questions regarding sexual orientation, use of the term “partner,” and using correct pronouns), communication and structural/administrative challenges faced by HCPs in providing care (HCP own fears and biases, transgender patient care, insurance issues, and procedural challenges for LGBT patients), and suggested recommendations from oncology HCPs to improve their care delivery for LGBT patients (more provider-based training, improving awareness of LGBT-friendly resources, establishing trusting relationships, and not assuming sexuality or gender identity). These findings have implications for developing and evaluating training programs to improve LGBT sensitivity and communication among HCPs, and encourage SOGI disclosure in an open and judgment-free health care environment.
The “Line Sign” Is a Rapid and Efficient Diagnostic “Test” for Morphea: Clinicopathological Study of 73 Cases
Authors: Yang Sarah , Draznin Michelle , Fung Maxwell A. 
Published: November 2018

Abstract:
Histologic clues that facilitate rapid diagnosis of morphea at scanning magnification have been described but not well studied. We examined 73 cases of morphea and 42 control cases to determine the sensitivity and specificity of a novel histopathological feature—“the line sign (LS)”—a prominent, straight interface between subcutis and adjacent collagen. The sensitivity of LS was shown to be the most sensitive feature among 4 other existing histopathological features. Its specificity, however, was not the highest among the other features and needs to be evaluated further in future studies to confirm the usefulness of LS as a diagnostic tool for morphea.
A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model
Authors: Gimble Jeffrey M. , Frazier Trivia , Wu Xiying , Uquillas Andrea Alarcon , Llamas Claire , Brown Theodore , Nguyen Doan , Tucker H. Alan , Arm Douglas M. , Peterson Dale R. , Bunnell Bruce A. 
Published: November 2018

Background:
Processed microvascular tissue (PMVT), a human structural allograft, is derived from lyophilized human tissue containing microcirculatory cellular components. Since PMVT serves as a source of extracellular matrix (ECM), growth factors, cytokines, and chemokines modulating angiogenesis, inflammation, apoptosis, and endogenous cell recruitment, we hypothesized its application would accelerate wound regeneration in a validated pressure ulcer (PU) model developed in C57BL/6 mice using two 24-hour cycles of skin ischemia/reperfusion created by placement and removal of external magnets.

Methods:
Two identical PU injuries (n = 50 female mice) were treated with (a) topical particulate PMVT, (b) injected rehydrated PMVT, or (c) saline control injection, and assessed daily for closure rates, scab formation/removal, and temperature. A baseline control cohort (n = 5) was euthanized at day 0 and treatment group cohorts (n = 5) were killed at 3, 7, or 14 days postinjury. The PU injuries were collagenase-digested for flow cytometric analysis of inflammatory, reparative, and stem cell frequencies and analyzed by hematoxylin and eosin (H&E) histology and immunofluorescence.

Results:
PMVT-accelerated wound closure, most notably, topical PMVT significantly increased mean closure from d5 (13% versus -9%) through d13 (92% versus 38%) compared with phosphate-buffered saline (PBS) controls (P < 0.05). PMVT also hastened scab formation/removal, significantly accelerated disappearance of inflammatory myeloid (CD11b+) cells while upregulating α-smooth muscle actin, vascular endothelial growth factor A, and placental growth factor and raised skin temperature surrounding the PU site, consistent with increased blood flow.

Conclusions:
These results indicate that PMVT has potential as an advanced treatment for restoring normal tissue function in ischemic wounds and merits clinical study.
Pancreatic Hormone Responses to Mixed Meal Test in New-onset Prediabetes/Diabetes After Non-necrotizing Acute Pancreatitis
Authors: Pendharkar Sayali A. , Singh Ruma G. , Bharmal Sakina H. , Drury Marie , Petrov Maxim S. 
Published: November 2018

Aim:
To investigate the pancreatic hormone responses to mixed meal test, in particular changes in insulin secretion, insulin sensitivity, and their interrelationship, in individuals with new-onset prediabetes or diabetes after non-necrotizing acute pancreatitis (NODAP) compared with healthy controls.

Methods:
Twenty-nine individuals with NODAP and 29 age-and sex-matched healthy controls were recruited. All participants (after fasting for at least 8 h) were given 12 oz. of BOOST drink and blood samples were collected before and after stimulation to measure insulin, C-peptide, glucagon, and pancreatic polypeptide. Indices of insulin sensitivity (HOMA-IS, 1/fasting insulin, Raynaud, and Matsuda) and insulin secretion (HOMA-β, Stumvoll, insulinogenic index 30ʹ and 60ʹ) were calculated. Repeated measures analyses were conducted in the unadjusted and adjusted models.

Results:
Insulin and C-peptide levels were significantly higher in individuals with NODAP compared with controls during mixed meal test in both the unadjusted (P=0.001 for both) and adjusted (P=0.004 and P=0.006, respectively) models. HOMA-IS (P=0.005), 1/fasting insulin (P=0.018), Raynaud index (P=0.018), and Matsuda index (P=0.021) were significantly lower in individuals with NODAP, whereas HOMA-β (P=0.028) and Stumvoll index (P=0.013) were significantly higher. Glucagon and pancreatic polypeptide levels did not differ significantly between NODAP and controls during mixed meal test in both the unadjusted (P=0.345 and P=0.206, respectively) and adjusted (P=0.359 and P=0.158, respectively) models.

Conclusions:
Decreased insulin sensitivity, β-cell compensation, and no significant change in postprandial levels of glucagon and pancreatic polypeptide characterize NODAP. The above findings may help develop an evidence-based protocol with a view to optimize control of glucose homeostasis in NODAP.
Xenogeneic transplantation and tolerance in the era of CRISPR-Cas9
Authors: Cowan Peter J. , Hawthorne Wayne J. , Nottle Mark B. 
Published: November 2018

Purpose of review
The use of genetically modified donor pigs has been integral to recent major advances in xenograft survival in preclinical nonhuman primate models. CRISPR-Cas9 gene editing technology has dramatically accelerated the development of multimodified pigs. This review examines the current and projected impact of CRISPR-Cas9-mediated donor modification on preventing rejection and potentially promoting tolerance of porcine xenografts.

Recent findings
CRISPR-Cas9 has been used to engineer several genetic modifications relevant to xenotransplantation into pigs, including glycosyltransferase knockouts (GGTA1, CMAH, β4GALNT2, A3GALT2 and combinations thereof), other knockouts (SLA-I, ULBP1, PERV and GHR), and one knock-in (anti-CD2 monoclonal antibody transgene knocked into GGTA1). Although the use of these pigs as donors in preclinical nonhuman primate models has been limited to a single study to date, in-vitro analysis of their cells has provided invaluable information. For example, deletion of three of the glycosyltransferases progressively decreased the binding and cytotoxicity of preexisting immunoglobulin G and immunoglobulin M in human sera, suggesting that this ‘triple-KO’ pig could be a platform for clinical xenotransplantation.

Summary
CRISPR-Cas9 enables the rapid generation of gene-edited pigs containing multiple tailored genetic modifications that are anticipated to have a positive impact on the efficacy and safety of pig-to-human xenotransplantation.
Detection of Calcified Aortic Plaques in an Apolipoprotein E Animal Model Using a Human Computed Tomography System for Ultra–High-resolution Imaging
Authors: Manta Calin , Meyer Mathias , Géraud Cyrill , Roscher Mareike , Goerdt Sergij , Schoenberg Stefan O. , Henzler Thomas , Ong Melissa M. 
Published: November 2018

Purpose:
The purpose of this study was to prospectively evaluate the feasibility of detecting calcified aortic plaques in apolipoprotein E knockout (ApoE-KO) mice using a state-of-the-art human computed tomography (CT) system.

Materials and Methods:
Eleven ApoE-KO and 9 wild-type mice, all male, were examined in this study. After intraperitoneal injection of 6.6% ketamine all mice underwent 2 ultra–high-resolution CT protocols on a third-generation dual-source CT system at 120 kVp and 130 kVp tube voltage, both performed with a tube current time product of 1300 mAs. Images (0.4 mm) with an increment of 0.2 mm were reconstructed using an iterative reconstruction algorithm. Calcium detectability and scores (Agatston, volume, mass) were determined with a dedicated human calcium scoring software (CaScoring). After the CT examination, a calcium quantification assay of the aortae was performed to determine the aortic calcium content of each mouse. The CT scan time ranged between 40 and 48 seconds. All mice survived the procedure.

Results:
Calcified plaques could be detected in 8 of 11 ApoE-KO mice. Quantification of calcium levels showed significant differences between those with morphologic calcium plaques detected in CT and those without (3.44±1.6 μg Ca2+/mg vs. 0.33±0.35 μg Ca2+/mg; P<0.05). The receiver-operating characteristics analysis revealed a total calcium cut-off value of 0.71 μg Ca2+/mg for the detection using calcium score algorithms (specificity: 100% and sensitivity: 90%).

Conclusion:
Using a state-of-the-art human CT protocol and an in-human–established calcium scoring system allows for the detection and quantification of calcified aortic plaques in ApoE-KO mice. These results may facilitate preclinical imaging for translational and longitudinal atherosclerotic research studies.
Circuit directionality for motivation: lateral accumbens-pallidum, but not pallidum-accumbens, connections regulate motivational attraction to reward cues
Authors: Smedley Elizabeth B. , DiLeo Alyssa , Smith Kyle S. 
Published: November 2018

AbstractSign-tracking behavior, in which animals interact with a cue that predicts reward, provides an example of how incentive salience can be attributed to cues and elicit motivation. The nucleus accumbens (NAc) and ventral pallidum (VP) are two regions involved in cue-driven motivation. The VP, and subregions of the NAc including the medial shell and core, are critical for sign-tracking, and connections between the medial shell and VP are known to participate in sign-tracking and other motivated behaviors. The NAc lateral shell (NAcLSh) is a distinct and understudied subdivision of the NAc, and its contribution to the process by which reward cues acquire value remains unclear. The NAcLSh has been implicated in reward-directed behavior, and has reciprocal connections with the VP, suggesting that NAcLSh and VP interactions could be important mechanisms for incentive salience. Here, we use DREADDs (Designer Receptors Exclusively Activated by Designer Drugs) and an intersectional viral delivery strategy to produce a biased inhibition of NAcLSh neurons projecting to the VP, and vice versa. We find that disruption of connections from NAcLSh to VP reduces sign-tracking behavior while not affecting consumption of food rewards. In contrast, VP to NAcLSh disruption affected neither sign-tracking nor reward consumption, but did produce a greater shift in animals’ behavior more towards the reward source when it was available. These findings indicate that the NAcLSh→VP pathway plays an important role in guiding animals towards reward cues, while VP→NAcLSh back-projections may not and may instead bias motivated behavior towards rewards.
Older adults sacrifice response speed to preserve multisensory integration performance
Authors: Jones Samuel A. , Beierholm Ulrik , Meijer David , Noppeney Uta 
Published: November 2018

AbstractAgeing has been shown to impact multisensory perception, but the underlying computational mechanisms are unclear. For effective interactions with the environment, observers should integrate signals that share a common source, weighted by their reliabilities, and segregate those from separate sources. Observers are thought to accumulate evidence about the world’s causal structure over time until a decisional threshold is reached.Combining psychophysics and Bayesian modelling, we investigated how ageing affects audiovisual perception of spatial signals. Older and younger adults were comparable in their final localisation and common-source judgement responses under both speeded and unspeeded conditions, but were disproportionately slower for audiovisually incongruent trials.Bayesian modelling showed that ageing did not affect the ability to arbitrate between integration and segregation under either unspeeded or speeded conditions. However, modelling the within-trial dynamics of evidence accumulation under speeded conditions revealed that older observers accumulate noisier auditory representations for longer, set higher decisional thresholds, and have impaired motor speed. Older observers preserve audiovisual localisation performance, despite noisier sensory representations, by sacrificing response speed.
Temperature-Jump Solution X-ray Scattering Reveals Distinct Motions in a Dynamic Enzyme
Authors: Thompson Michael C. , Barad Benjamin A. , Wolff Alexander M. , Cho Hyun Sun , Schotte Friedrich , Schwarz Daniel M.C. , Anfinrud Philip , Fraser James S. 
Published: November 2018

AbstractCorrelated motions of proteins and their bound solvent molecules are critical to function, but these features are difficult to resolve using traditional structure determination techniques. Time-resolved methods hold promise for addressing this challenge but have relied on the exploitation of exotic protein photoactivity, and are therefore not generalizable. Temperature-jumps (T-jumps), through thermal excitation of the solvent, have been implemented to study protein dynamics using spectroscopic techniques, but their implementation in X-ray scattering experiments has been limited. Here, we perform T-jump small- and wide-angle X-ray scattering (SAXS/WAXS) measurements on a dynamic enzyme, cyclophilin A (CypA), demonstrating that these experiments are able to capture functional intramolecular protein dynamics. We show that CypA displays rich dynamics following a T-jump, and use the resulting time-resolved signal to assess the kinetics of conformational changes in the enzyme. Two relaxation processes are resolved, which can be characterized by Arrhenius behavior. We also used mutations that have distinct functional effects to disentangle the relationship of the observed relaxation processes. A fast process is related to surface loop motions important for substrate specificity, whereas a slower process is related to motions in the core of the protein that are critical for catalytic turnover. These results demonstrate the power of time-resolved X-ray scattering experiments for characterizing protein and solvent dynamics on the μs-ms timescale. We expect the T-jump methodology presented here will be useful for understanding kinetic correlations between local conformational changes of proteins and their bound solvent molecules, which are poorly explained by the results of traditional, static measurements of molecular structure.
Ignoring stratigraphic age uncertainty leads to erroneous estimates of species divergence times under the fossilized birth-death process
Authors: Barido-Sottani Joëlle , Aguirre-Fernández Gabriel , Hopkins Melanie , Stadler Tanja , Warnock Rachel 
Published: November 2018

AbstractFossil information is essential for estimating species divergence times, and can be integrated into Bayesian phylogenetic inference using the fossilized birth-death (FBD) process. An important aspect of palaeontological data is the uncertainty surrounding specimen ages, which can be handled in different ways during inference. The most common approach is to fix fossil ages to a point estimate within the known age interval. Alternatively, age uncertainty can be incorporated by using priors, and fossil ages are then directly sampled as part of the inference. This study presents a comparison of alternative approaches for handling fossil age uncertainty in analysis using the FBD process. Based on simulations, we find that fixing fossil ages to the midpoint or a random point drawn from within the stratigraphic age range leads to biases in divergence time estimates, while sampling fossil ages leads to estimates that are similar to inferences that employ the correct ages of fossils. Second, we show a comparison using an empirical dataset of extant and fossil cetaceans, which confirms that different methods of handling fossil age uncertainty lead to large differences in estimated node ages. Stratigraphic age uncertainty should thus not be ignored in divergence time estimation and instead should be incorporated explicitly.
Two- and three-dimensional coordination polymers based on zinc(II) and furan-2,5-dicarboxylic acid: structure variation due to metal-to-linker ratio
Authors: Mao Yimin , Zavalij Peter Y. 
Published: November 2018

Two ZnII-based coordination polymers (CPs) were synthesized by the hydrothermal method, using Zn(NO3)2·6H2O and furan-2,5-dicarboxylic acid (FDCA) in dimethylformamide (DMF) solvent, at 95 °C. Poly[tetrakis(dimethylazanium) [tetrakis(μ2-furan-2,5-dicarboxylato-κ2
O
2:O
5)dizinc(II)]], {(C2H8N)4[Zn2(C6H2O5)4]}
n
or {[DMA]4[ZnII
2(FDC)4]}
n
(DMA = dimethylazanium and FDC = furan-2,5-dicarboxylate), (1), was obtained with a 1:1 molar ratio of ZnII and FDCA. It crystallized in the monoclinic space group C2/c. Coordinated by ZnII ions, FDC2− ligands form 21 double-stranded helices propagating along the b axis. The helices are interconnected and extend laterally in the a direction, forming a two-dimensional (2D) sheet-like network. The 2D sheets are stacked along the c direction without interconnections. DMA cations are cocrystallized in (1) and are hydrogen bonded with carboxylate O atoms of the FDC2− ligands. The hydrogen-bonding pattern consists of R
2
2(4) and R
2
2(10) motifs alternating in a chain. Poly[bis(dimethylazanium) [bis(μ4-furan-2,5-dicarboxylato-κO
2:κO
2′:κO
5:κO
5)bis(μ3-furan-2,5-dicarboxylato-κO
2:κO
2′:κO
5)dizinc(II)] dimethylformamide 3.08-solvate], {(C2H8N)2[Zn2(C6H2O5)4]·3.08C3H7NO}
n
or {[DMA]2[ZnII
3(FDC)4]·3.08DMF}
n
, (2), was obtained with a 1:2 molar ratio of ZnII and FDCA. It crystallized in the monoclinic space group P21/c, forming a three-dimensional network. The pores are filled with DMA cations and DMF solvent molecules.
Simulation the effects of cross-flow injection on the slip velocity and temperature domain of a nanofluid flow inside a microchannel
Authors: Jalali Esmaeil , Karimipour Arash 
Published: November 2018

Purpose
In this paper, the forced convection heat transfer of the nanofluid composed of water and AL2O3 nanoparticles is simulated in a two-dimensional horizontal microchannel by injecting the lower wall. The upper wall of the microchannel is 303 K at temperature TH. On the lower wall of the microchannel, there are three holes for flow injection. Other parts of the wall are insulated. In this paper, the effect of parameters such as Reynolds number, slip coefficient and volume fraction of nanoparticles is investigated.

Design/methodology/approach
The boundary condition of the slip velocity is considered on the upper and lower walls of the microchannel. In this work, the flow of nanofluid in the microchannel is considered to be slow, permanent and Newtonian. In the present study, the effect of injection through the microchannel wall on the slip velocity is examined for the first time.

Findings
The results are also presented as velocity profiles and Nusselt number diagrams. It was found that the Nusselt number increases with increasing the amount of slip coefficient of velocity and the weight percentage of solid nanoparticles. The rate of this increase is higher in the high values of the Reynolds number.

Originality/value
A novel paper concerned the simulation of cross-flow injection effects on the slip velocity and temperature domain of a nanofluid flow inside a microchannel.