bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and Characterization Indigenous of Lactobacillus spfrom Bovine Rumen Fluidof Slaughterhouse
Authors: Nurhajati Tri , Soepranianondo Koesnoto , Lokapirnasari Widya Paramita , Sahidu Adriana Monica 
Published: March 2018

The discovery and characterisation of indigenous lactic acid bacteria (LAB) are important for diversity microbes as candidate probiotic. This research was aimed to identify lactic acid bacteria isolate from isolation process of local bovine rumen fluid from slaughterhouse in Surabaya Indonesia. Genotypic testing was conducted by analyzing 16S rDNA and biochemical identification. DNA of sample isolate was isolated and then amplified in vitro through the PCR method. Determination of nucleotide sequence of 16S rDNA was performed with sequencing method. The result of nucleotide sequence was than compared with GenBank database. The BLAST was then applied to identify the phylogenetic tree. Based on the biochemical characterization and nucleotide sequences, that isolate was identified as Lactobacillus rhamnosus subsp TG15. The result of this research showed that L.rhamnosus subsp TG15 showed viability bacteria in MRSA as control as much as 1.1 x 108 CFU/ml, mean while in MRSA pH 2, L.rhamnosus subsp TG15 showed its viability as much as 9.3 x 106 CFU/ml. Viability of isolate on bile tolerance 0.3% was 2.4 x 107 CFU/ml). Index antagonist bacteria test on S.aureus showed inhibition diameter as much as 2.0 mm and in antagonist test on E.coli as much as 2.5 mm. Based on the result, it could be concluded that this research found a new strain of lactic acid bacteria, L.rhamnosus subsp TG15 and that isolate has ability as the probiotic candidate. Keywords: L.rhamnosus subsp TG15; survival on acidity; bile salts; S.aureus and E.coli
Protective Effect of Propolis Extract Against Lead Acetate Toxicity in Mice (Mus Musculus) Testes
Authors: Widawati Tuti , Sudjarwo Sri Agus , Hermadi Herry Agoes 
Published: March 2018

This study was aimed to investigate the protective effect of propolis extract from Apis mellifera that was obtained from Agro Tawon Rimba Raya Malang against the exposure of lead acetate 20 mg/kgBW orally. Twenty-five BALB/C mice were randomly devided into five groups. Negative control received only CMC-Na 1.5 % and Tween 80 0.5% also aquadest an hour after the first administration; Positive control group that administered CMC-Na 1.5 % and Tween 80 0.5% then continued received 20 mg/kgBW of lead acetate, Treatment group was received 200; 400 and 800 mg/kgBW of propolis first and then an hour after that received 20 mg/kgBW of lead acetate. The treatment was conducted for 7 days of adaptation and 35 days of treatment. At the end of the research all mice were sacrificed and testes were collected. Testes tissue were processed using Hematoxylin-Eosin staining. The result showed that an increase of spermatocyte, spermatid and sertoli cell compare to group that only received lead acetate, but thickness of seminiferous tubules epithelium showed slighly similar in all groups. Key words : propolis, lead, reactive oxygen species, free radical, testes
Potency of Bacillus cereus WPL 415 to Increase Crude Protein and Decrease Crude Fiber of Animal Feed Stuff
Authors: Lokapirnasari Widya Paramita , Sahidu Adriana Monica , Nurhajati Tri , Soepranianondo Koesnoto , Yulianto Andreas Berny 
Published: March 2018

This research aims to identify isolate as a probiotic candidate derived from liquor rumen of local beef cattle and to know the ability of isolates as biofermentor on basal feed to the changes in the nutrient value. The selected samples were obtained from a slaughterhouse in Surabaya. This study consisted of two stages. The first stage was the identification of bacteria through the test of morphology, Gram staining, biochemical, resistance to acidity and 16S rDNA sequencing. The second stage was a test of the ability of the isolates on the nutrient of basal feeds by fermentation for three days in an aerobic condition. Based on the findings of the first phase, it has been identified that probiotic bacterium rods, motility positive, Gram-negative, have viability at pH 2 and pH 3 for 90 minutes and 24 hours and have the ability to ferment lactose, sucrose, galactose, ribose, cellobiose and xylose. Furthermore, based on test results of 16S rDNA sequencing, the probiotic bacterium was identified as Bacillus cereus WPL 415. Based on the research results at the second stage, Bacillus cereus WPL 415 at doses of 0.25% and 0.5% could improve the nutrient content of the basal feed. The results of the proximate analysis revealed that there was an increase in crude protein content of 6.78% until 8.12% compared to the control and was able to lower the crude fiber content of 15.19% and 17.40% compared to the control. Based on these results it can be concluded that Bacillus cereus WPL 415 from local beef cattle can be used as a probiotic candidates to improve the quality of animal feed. Keywords: Bacillus cereus, probiotic, crude protein, crude fiber
Acute Toxicity Tests of Alkaloid Pare (Momordica Charanthia) Fruit on The Histopathology of Liver
Authors: Wurlina Wurlina , Meles Dewa Ketut , Zakaria Sunarni , Mustofa Imam , Susilowati Suherni , Adnyana I Dewa Putu Anom 
Published: March 2018

Pare fruit (Momordica charanthia) potential as an antidiabetic. In preliminary research has proven that alkaloid of the Pare fruit (Momordica charanthia) can lower blood sugar levels of mice suffering from diabetes mellitus. Alkaloid Pare can improve pancreatic β-cell function by improving the preparation phase for the cell dividing (interphase) and repair the mitotic stages and increase the CDK expression in mice with diabetes mellitus. The purpose of this research was to determine the dose that causes the death of 50% (LD50) and to determine the toxicity effect of alkaloid Pare (Momordica charanthia) against damage in the form of congestion, degeneration, and necrosis of liver cells. Acute toxicity test with a 24-hour long treatment using 60 female mice, divided into six groups, each group consisted of 10 animals. The group is as follows: P0 only given distilled water, the group P1, P2, P3, P4 and P5 respectively treated 0.3 g/kg bw, 1 g/kg bw, 3 g/kg bw, 10 g/kg bw and 15 g/kg bw. Furthermore, all the mice were necropsied to take the liver for histopathology preparations to observe the occurrence of congestion, degeneration, and necrosis. The parameters observed through histopathological examination of the liver include congestion, degeneration, and necrosis. The results of the research tested by Kruskal-Wallis test with data obtained based on the value of scoring was not significantly different (α<0.05) in the liver histopathological changes in the form of congestion, degeneration, and necrosis between control and treatment groups orally in time 24 hours. The dose can cause 50% of deaths more than 15 g in mice are included in the category of medicinal substances which are not harmful. Keywords: Alkaloid Pare fruit, LD50, congestion, degeneration, necrosis, liver.
Teratogenic Effect of Congenital Toxoplasmosis in Chicken Embryo
Authors: Suwanti Lucia Tri , Mufasirin Mufasirin , Plumeriastuti Hani , Safitri Erma 
Published: March 2018

This research is designed to observe the teratogenic effect of Toxoplasma gondii infection in chick embryos, based on the number of somites, embryo length and the development of embryonic brain vesicles. Methods in the research: Chicken eggs were infected with 1 x 103 tachyzoites of T. gondii. The eggs were incubated in eggs hatching box. Observation of somite performed on embryonated eggs 24 hours after incubation and the embryonic development of vesicles performed 72 hours after incubation then the length of each embryo were measured. Results: Revealed that there was a significant difference in the number of somites (p < 0.1), T. gondii infection reduced the number of somites. While in the number of brain vesicles in 3 - days old chicken embryos, although there was no significant difference, the size declining emerged. The length of the embryos both at 24 or 72 hours old showed that T. gondii infection reduced the length (p < 0.1). Conclusions: T. gondii infection influences the development of chicken embryos in the declining of length and the decreasing of somite embryo number. Keywords: IGF-I crossbreed mare serum pregnant; Follicle; Mus musculus
Case Study : Dystocia on Beef Cattle in Kunir Regency of Lumajang District, East Java, Indonesia in 2015 and 2016
Authors: Febrianila Rosiana , Lokapirnasari Widya P , Restiadi Tjuk I , Mustofa Imam , Hermadi Herry A , Safitri Erma 
Published: March 2018

Dystocia defined as difficulty of birth. Cattle that experienced dystocia characterized by extended processing time of birth, difficult, and impossible to do without help of human stem. This study aimed to determine the number and causative factor of dystocia in beef cattle in Kunir sub district, Lumajang district. Data acquisition used primary data and secondary data. Primary data was obtained from direct observations about management of maintenance, then interviewed the farmers in Kunir sub district. Secondary data was data obtained from the recording belongs to animal health technical officer. The results showed that the prevalence of dystocia in Kunir district, Lumajang district as many as 63 cases or 11,6 % of 543 births. The result of the research is analyzed using chi square (χ2) method on SPSS 20.0 program and risk factors that increase the incident of dystocia were IB semen which greater than the cattle site, the position of the fetus and inertia uteri can result in weakness of the catrle at the age of older and more likely to give birth. Key words: Dystocia; beef catlle
The Effectiveness of Honey in Physiological Nacl to Maintain of Viability and Motility of Spermatozoa
Authors: Agustina Elsa , Hermadi Herry Agoes , S Hario Puntodewo , Hernawati Tatik , Triana Indah Norma , Safitri Erma 
Published: March 2018

This research was conducted to determine whether there is influence of various concentrations of mead physiological saline as diluent on the viability and motility fat-tailed sheep during storage 5°C. This research is compiled using the trial completely randomized design (CRD). Differences in the treatment of thinning the honey in physiological saline are PO (0%), P1 (0.2%), P2 (0.4%), P3 (0.6%), P4 (0.8%). The treatment stored in a refrigerator at a temperature of 5°C and then observed every 0, 24, and 48 hours. Data were analyzed by ANOVA followed by Duncan's Multiple Range Test. The conclusions obtained from this research there is the effect of adding various concentrations of NaCl physiological mead on the viability and motility fat tailed sheep during storage 5°C and consentration mead NaCl physiological to maintain viability and motility best tail sheep are treated P3 (0.6% solution = 0.6 ml honey in 99.4 ml physiological NaCl) at the time T24 (24 hours). Key words: Fat-Tailed Sheep, Honey, Motility, Physiological NaCl, Spermatozoa, Viability
Utilization of Sumbawa Tropical Forest Honey Apis Dorsata to Improve Fertility of Indonesia Oriental Magpie Robin (Copsychus saularis) as Effort Animal Population Increasement
Authors: Hasib Abdullah , Muhamad Risaldi , Reksa Talita Yuanda , Artha Alvina Ulimaz , Safitri Erma 
Published: March 2018

The objective of this experiment is to observe the effect of forest honey produced by giant bees Apis dorsata on improving fertility and egg number of the bird. The bird which observed in this experiment is captive bird Oriental Magpie Robin (Copsychus saularis). Twelve paired captive bird in official captivity were transferred into new pairing cage and adapted along two weeks. Every bird was fed with crickets and normal ration mixed with low protein diet (LP) with 16% of crude protein gradually. After an adaptation period, twelve paired captive bird were divided into three groups T1, T2 and T3. Birds were treatened with honey solution 5%, 10% and 15% concentration series in 1 ml aquadest intraabdominal of 10 crickets and fed with 15 crickets and 20 gram low protein ration (16%) per day for two laying periods. Egg production each group were calculated and observed its fertility among all egg production in one parental. In accordance with that, the results were analyzed descriptively. This pre-treatment, the bird’s mating behaviour becomes a week faster than the normal period. Bird’s fertility is also increasing with the provision of honey in the bird nutrition. In the bird’s pairs in cage’s number 9 and 10 have shown development by producing 3 fertile eggs after being treated with honey. In the previous period showed from 3 eggs only 1 being fertile. Honey has not been able to increase the number of eggs produced by birds. However, there is an enhancement in the animal population up to 25% of the 12 pairs of birds that are used as experimental samples in the captivity. The conclusion of this study shows that honey can accelerate the mating behaviour of birds and can increase the fertility of birds' eggs. However, honey has not yet affected the increase the quantity of the birds' egg. Longer observation’s duration with extra bird’s sample is needed for future research, in order to see the reproduction cycle of birds over several periods. Field research related to nutrition in chemistry nutrition’s fields related the active ingredient in honey that can affect the reproduction system is also important to conducted for further research. Keywords: Oriental Magpie Robin; Eggs Production; Fertility; Mating Behaviour; Honey
Utilization of Honey Apis dorsata as Antiosteoporosis on Requirements of Bone Calcium Ash Density on Ovariohysterectomized White Rat (Ratus norvegicus)
Authors: Ibrahim Muhammad Huda Ramadhan , Hasib Abdullah Hasib , Rohmah Siti Nur , Abani Salsabilla , Yordan Samsi , Yudaniayanti Ira Sari 
Published: March 2018

The purpose of this study was to determine the effect of honey bee Apis dorsata as anti-osteoporosis in calcium ash density (CAD) of bone in osteoporotic-induced rats. The target of this study was to know bone calcium levels after being given honey bees Apis dorsata. In this study, 35 female white rats (Ratus norvegicus) mature was used with weight 200gr. Divided into 5 groups, 2 control groups and 3 treatment groups. The negative control group (SH) was not induced by osteoporosis and was given the only aquadest of 1.5 ml/day. Whereas the positive control group was induced by osteoporosis (OH) and was given only aquadest 1.5 ml/day. T1, T2 and T3 treatment groups were given bee honey with various doses including 1g / kg ad 1.5 ml aquadest, 2g / kg BB ad 1.5 ml aquadest and 3g / kg BB ad 1.5 ml aquadest. Then after 12 weeks, white rats were sacrificed for lumbar vertebrae. Furthermore, the sixth lumbar spine os vertebrae will be examined for calcium bone ash content. The data were obtained was analyzed using statistical analysis of variance (ANOVA) for the results of the calcium ash content data Keywords: Honey; Osteoporosis; CAD
Increased Integrity of Plasma Membrane and Acrosome Cap Spermatozoa Limousin Cattle at Post Thawing in Frozen Media by adding Seawater Extract
Authors: Faidah Nur , Hernawati Tatik , Lamid Mirni , Ismudiono Ismudiono , Suprayogi Tri Wahyu , Mulyati Sri 
Published: March 2018

This research was determined membrane integrity and acrosome cap of Limousin bull post thawing after adding seawater extract with different concentrations in extender skim milk and egg yolk to increased frozen semen quality. This research used fresh samples of Limousin bull’s semen collected by using artificial vagina, then devided into 4 treatments and 6 replications. The experimental design that used was Complete Random Design. Analysis of the data using Analysis of Variant (ANOVA) one way then proceeds to the Duncan Multiple Range Test to determine significant differences between treatments.The first treatment P0 no seawater extract added as a control. The second treatment P1 was treated with 0.109 µL seawater extract, P2 was treated with 0.426 µL seawater extract, and P3 was treated with 1.09 µL seawater extract. The result showed that determine significant differences between treatments. The post thawing membrane integrity’s result was P0= 22.00 ± 4.28, P1= 22.66 ± 3.61, P2= 25.00 ± 2.75, and P3= 29.00 ± 1.67. The post thawing acrosome cap’s result was P0= 30.50 ± 1.37, P1= 31.50 ± 3.27, P2= 34.83 ± 2.31, and P3= 38.00 ± 1.41. The highest concentration added seawater extract to increased membrane integrity and acrosome cap spermatozoa in this research was 1.09 µL. Keywords: Limousin bull; spermatozoa; seawater extract; membrane integrity; acrosome cap
The Relation of Body Temperature and Vaginal Cytology Examination in Time Artificial Insemination Rate Fat-tailed Sheep (Ovis Aries) in The District Sidoarjo East Java
Authors: Anggriawan Rhendyka Prasetya , Utama Suzanita , Eliyani Hana 
Published: March 2018

Ewes fertility is one aspect that needs to maximum level. Reproductive performance in sheep livestock in Indonesia has become one of the main determinants of reproductive success. This study has been carried out of the body temperature measurement and sampling of vaginal cytology of 60 fat-tailed sheep before artificial insemination in Sidoarjo East Java. Body temperature measurement is done in the rectum of reported ewes in heat and artificial insemination requested can be determined by observing the condition of the vulva of ewes such as the presence or absence of mucus through the vagina, vulva swollen and reddened vulva mucosa. vaginal cytology sampling with a cotton swab inserted into the vulva ± 15cm of ewes that reported in heat then interpreted by a microscope. The result showed that ewe’s body temperature in 39.1oC-39.2oC has a significant correlation with vaginal cytology type on oestrous phase. Keyword: Ewes, body temperature, vaginal cytology, the district Sidoarjo East Java
Effect of Laser Acupuncture Shoot on Ova Point of Male Mojosari Duck (Anas plathyrhynchos) on The Number of Sertoli and Leydig Cells
Authors: Adikara Yuanara AR , Samik Abdul , Yudaniayanti Ira S , Adikara Tatang S , Hestianah Eka P , Utama Suzanita 
Published: March 2018

Demand for duck eggs and meat recently increased along with the increasing interest of the community to consume duck eggs and meat. Due to that reason researcher try to find the best way to enhance the reproductive ability of livestock., which is in this research by laser puncture shot. Eighteen ducks were allocated into three groups, the control group, the first treatment group with laserpuncture shoot dose of 0.2 Joule (J), and the second treatment group with laser puncture shoot dose of 0.4 J. Data were analyzed with ANOVA and further tested by Duncan multiple range test. Research result showed that laser puncture shoot with a dose of 0.2 J and 0.4 J decreased the number of Sertoli and Leydig cells. Keywords: Mojosari duck; laser acupuncture; Sertoli cells; Leydig cells
Insulin-Like Growth Factor-I (IGF-I) from Crossbred Pregnant MareSerum to Increase Follicle Number of Mice (Mus musculus)
Authors: Abdullah Abdullah , IRestiadi Tjuk , Lastuti Nunuk DR , Damayanti Tita , Wurlina Wurlina , Safitri Erma 
Published: March 2018

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subjects of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatments were C0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are the number of primary, secondary, tertiary and de Graff follicles. During the treatment, the estrous cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA) and followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affects (p<0.05) on increasing the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicles significantly (p<0.05). Keywords: IGF-I crossbreed mare serum pregnant; follicle; Mus musculus
Morphological Identification Nematodes Tanqua tiara Found on Gastric Varanus salvator at East Java
Authors: Agustin Alfiana Laili Dwi , Koesdarto Setiawan , Lukiswanto Bambang Sektiari , Suwanti Lucia Tri , Arifin Zainal , Putranto Emmanuel Djoko 
Published: March 2018

Tanqua tiara (T. tiara) is the gastric nematode of Varanus salvator (V. salvator) [1-4], this study was conducted to identification morphology of T. tiara. Although there are no reports of human and animal infection T.tiara but still have the potential to transmit the disease to humans through direct contact or indirectly. Isolation of adult worms T. tiara from the digestive tract of V. salvator and then will make to dry preparat.Some 20 V.alvator's gastrointestinal tract at autopsy and gained as much as 321 nematode worms, nematode worms were obtained from the stomach V. salvator, result identification from dry preparat show : Male (mm) (Total length of body 9.4-32, Wide body 0,26-1,77, Diameter of head-bulb 0,17-0,32, The length of the head 0,23-0,33, Distance from the head-end to the end of the esophagus 2,8-5,7, Distance from the head-end to cervical sac 0,22-0,81, The thickness of the cuticle 0,008-0,036, Long tail 0,13-0,42, Long spikula0,3-1,1) and female (mm) (Total length of body 6,8-22, Wide body 0,14-2,33, Diameter of head-bulb 0,15-0,34, The length of the head 0,1-0,28, Distance from the head-end to the end of the esophagus 2,3-4,58, Distance from the head-end to cervical sac 0,18-0,88, The thickness of the cuticle 0,007-0,031, Long tail 0,12-0,28, Distance vulva from the tail end 3,02-3,61, Long uterus 13,1-21,4, size of egg 0,04-0,052X0,03-0,049). Identification of morphological Tanqua tiara indicate worms vary in size, but the specimen is dominated by small-sized worms. Keywords: Varanus salvator; Tanqua tiara; Cephalic bulb.
Effect of Propolis on Spermatogenic Cells Number and Diameter of Seminiferous Tubules in Male Mice (Mus musculus)
Authors: Nurkarimah Dona Astari , Hestianah Eka Pramyrtha , Wahjuni Retno Sri , Hariadi Mas’ud , Kuncorojakti Suryo , Hermadi Herry Agoes 
Published: March 2018

The present research aimed to determine the effect of propolis in spermatogenic cells number and seminiferous tubules diameter. Group P0 served as control group, P1 (1,6 mg/0,5ml/day), P2 (3,2 mg/0,5 ml/day), P3 (6,4 mg/0,5 ml/day), and P4 (12,8 mg/0,5 ml/day) were given propolis ethanolic extract treatment orally and killed after 14 days. Spermatogenic cells number (spermatogonial cells, primary spermatocytes, spermatids) and seminiferous tubules diameter were observed. The result showed a lower number of spermatogonial in P1 and P2 groups, primary spermatocytes reduction in P2 group, spermatids increased in P1, P3, and P4 group. Seminiferous tubules diameter decreased in P2 and P3 group. P2 group (3,2 mg/0,5 ml/day) showed the lowest result in all parameters (p<0.05). However, oral administration of propolis at these dose for 14 days may decrease spermatogonial cells and primary spermatocytes and increase spermatids number in adult mice. Keywords: Propolis; seminiferous tubule; spermatogenic cells; testis histology