bluuebunny/crossref_metadata_2025_split huggingface dataset:
Applications of a Novel Knowledge Discovery and Data Mining Process Model for Metabolomics
Authors: BaniMustafa Ahmed , Hardy Nigel 
Published: August 2019

ABSTRACTThis work demonstrates the execution of a novel process model for knowledge discovery and data mining for metabolomics (MeKDDaM). It aims to illustrate MeKDDaM process model applicability using four different real-world applications and to highlight its strengths and unique features. The demonstrated applications provide coverage for metabolite profiling, target analysis, and metabolic fingerprinting. The data analysed in these applications were captured by chromatographic separation and mass spectrometry technique (LC-MS), Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance spectroscopy (NMR) and involve the analysis of plant, animal, and human samples. The process was executed using both data-driven and hypothesis-driven data mining approaches in order to perform various data mining goals and tasks by applying a number of data mining techniques. The applications were selected to achieve a range of analytical goals and research questions and to provide coverage for metabolite profiling, target analysis, and metabolic fingerprinting using datasets that were captured by NMR, LC-MS, and FT-IR using samples of a plant, animal, and human origin. The process was applied using an implementation environment which was created in order to provide a computer-aided realisation of the process model execution.
Anatomical development of the cerebellothalamic tract in embryonic mice
Authors: Dumas Daniël B. , Gornati Simona V. , Adolfs Youri , Shimogori Tomomi , Pasterkamp R. Jeroen , Hoebeek Freek E. 
Published: August 2019

AbstractCerebellar projections to the thalamus are a pivotal connection in cerebello-cerebral interactions. Apart from its role in coordinating sensorimotor integration in the adult brain, the cerebello-thalamic projection has also been implemented in developmental disorders, such as autism spectrum disorders. Although the development of the cerebellum, thalamus and cerebral cortex have been studied in many species, a detailed description of the ontogeny of the mammalian cerebello-thalamic tract (CbT) is currently missing. Here we investigated the development of the CbT at embryonic stages using transgenic Ntsr1-Cre/Ai14 mice and in utero electroporation (IUE) of wild type mice. Wide-field, confocal and 3D light-sheet imaging of immunohistochemical stainings showed that CbT fibers arrive in the prethalamus between E14.5 and E15.5, but only invade the thalamus after E16.5. We quantified the spread of CbT fibers throughout the various thalamic nuclei and found that at E17.5 and E18.5 the ventrolateral, ventromedial and parafascicular nuclei, but also the mediodorsal and posterior complex become increasingly innervated. Several CbT fiber varicosities colocalize with vGluT2, indicating that already from E18.5 the CbT synapse in various thalamic nuclei. Our results contribute to the construction of a frame of reference on the anatomical development of the CbT, which will help to guide future experiments investigating neurodevelopmental disorders.Significance statementUsing various microscopic approaches, we investigated the anatomical development of the fiber tract between the cerebellum and thalamus, one of the major mammalian brain connections. Our results show that in mice cerebellar axons wait outside of the thalamus from embryonic day (E)15.5 until E17.5 before invading the thalamic complex. Cerebellar axons establish vGluT2-positive synapses at E18.5 throughout various thalamic nuclei, each of which subsequently develops its connections with dedicated cerebral cortical regions. Our data thereby advocate the cerebellar influence on the maturation of the thalamus and connected cerebral cortex. This knowledge can help to guide future experiments into neurodevelopmental disorders affecting cerebello-thalamo-cortical networks.
Degradation of the incretin hormone Glucagon-Like Peptide-1 (GLP-1) byEnterococcus faecalismetalloprotease GelE
Authors: LeValley Stephanie L. , Tomaro-Duchesneau Catherine , Britton Robert A. 
Published: August 2019

AbstractMetabolic diseases, including Type 2 Diabetes and obesity, have become increasingly prevalent global health concerns. Studies over the past decade have established connections between the gastrointestinal microbiota and host metabolism, but the mechanisms behind these connections are only beginning to be understood. We were interested in identifying microbes that have the ability to modulate the levels of the incretin hormone glucagon like peptide 1 (GLP-1). Using a human derived cell line that is capable of secreting GLP-1 in response to stimulatory ligands (NCI-H716), we identified supernatants from several bacterial isolates that were capable of decreasing GLP-1 levels, including several strains ofEnterococcus faecalis. We further identified the secreted protease GelE, an established virulence factor fromE. faecalis, as being responsible for GLP-1 inhibition via direct cleavage of GLP-1 by GelE. Finally, we demonstrated thatE. faecalissupernatants can disrupt a colonic epithelial monolayer and cleave GLP-1 in agelEdependent manner. This work suggests that a secreted factor from an intestinal microbe can traverse the epithelial barrier and impact levels of an important intestinal hormone.ImportanceHumans have a complex and interconnected relationship with their gastrointestinal microbiomes, yet our interest in the microbiome tends to focus on overt pathogenic or probiotic activities, leaving the roles that commensal species may have on host physiology and metabolic processes largely unexplored. Commensal organisms in the microbiome produce and secrete many factors that have an opportunity to interact with the gastrointestinal tract and host biology. Here we show that a secreted protease fromE. faecalis, GelE, is able to degrade the gastrointestinal hormone GLP-1, which is responsible for regulating glucose homeostasis and appetite in the body. The disruption of natural GLP-1 signaling by GelE may have significant consequences for maintaining healthy blood glucose levels and in the development of metabolic disease. Furthermore, this work deepens our understanding of specific host-microbiome interactions.
N-Acetyl Cysteine Alleviates Coxsackievirus B-Induced Myocarditis by Suppressing caspase-1
Authors: Wang Yao , Zhao Shuoxuan , Chen Yang , Wang Ying , Wang Tianying , Wo Xiaoman , Dong Yanyan , Zhang Jian , Xu Weizhen , Qu Cong , Feng Xiaofeng , Wu Xiaoyu , Wang Yan , Zhong Zhaohua , Zhao Wanran 
Published: August 2019

AbstractViral myocarditis caused by Coxsackievirus B (CVB) infection is a severe inflammatory disease of the myocardium, which may develop to cardiomyopathy and heart failure. No effective medicine is available to treat CVB infection. Here we evaluated the anti-CVB effect of N-acetyl cysteine (NAC), a widely used antioxidant. NAC significantly alleviated myocarditis and improved the overall condition of CVB type 3 (CVB3)-infected mice. Importantly, NAC treatment suppressed viral replication in both myocardium and cell culture. We show that NAC inhibited CVB3 replication when it was applied at the early stage of CVB3 infection. NAC’s antiviral mechanism, while independent of its antioxidant property, relies on its inhibition on caspase-1 activation, since the knockdown of caspase-1 blocked CVB3 replication. Moreover, NAC promotes procaspase-1 degradation via ubiquitin proteasome system, which may further contribute to the inhibited activity of caspase-1. NAC also inhibits the activity of viral proteases. Taken together, this study shows that NAC exerts potent anti-CVB effect by inhibiting caspase-1 and viral proteases. This study suggests that NAC can be a safe therapeutic option for CVB-induced myocarditis.
Polygenic patterns of adaptive introgression in modern humans are mainly shaped by response to pathogens
Authors: Gouy Alexandre , Excoffier Laurent 
Published: August 2019

AbstractAnatomically modern humans carry many introgressed variants from other hominins in their genomes. Some of them affect their phenotype and can thus be negatively or positively selected. Several individual genes have been proposed to be the subject of adaptive introgression, but the possibility of polygenic adaptive introgression has not been extensively investigated yet. In this study, we analyze archaic introgression maps with refined functional enrichment methods to find signals of polygenic adaptation of introgressed variants. We first apply a method to detect sets of connected genes (sub-networks) within biological pathways that present higher-than-expected levels of archaic introgression. We then introduce and apply a new statistical test to distinguish between epistatic and independent selection in gene sets of present-day humans. We identify several known targets of adaptive introgression, and we show that they belong to larger networks of introgressed genes. After correction for genetic linkage, we find that signals of polygenic adaptation are mostly explained by independent and potentially sequential selection episodes. However, we also find some gene sets where introgressed variants present significant signals of epistatic selection. Our results confirm that archaic introgression has facilitated local adaptation, especially in immunity-related and metabolic functions and highlight its involvement in a coordinated response to pathogens out of Africa.
Young consumers’ brain responses to pop music on Youtube
Authors: Cha Kyoung Cheon , Suh Minah , Kwon Gusang , Yang Seungeun , Lee Eun Ju 
Published: August 2019

PurposeThe purpose of this paper is to determine the auditory-sensory characteristics of the digital pop music that is particularly successful on the YouTube website by measuring young listeners’ brain responses to highly successful pop music noninvasively.Design/methodology/approachThe authors conducted a functional near-infrared spectroscopy (fNIRS) experiment with 56 young adults (23 females; mean age 24 years) with normal vision and hearing and no record of neurological disease. The authors calculated total blood flow (TBF) and hemodynamic randomness and examined their relationships with online popularity.FindingsThe authors found that TBF to the right medial prefrontal cortex increased more when the young adults heard music that presented acoustic stimulation well above previously defined optimal sensory level. The hemodynamic randomness decreased significantly when the participants listened to music that provided near- or above-OSL stimulation.Research limitations/implicationsOnline popularity, recorded as the number of daily hits, was significantly positively related with the TBF and negatively related with hemodynamic randomness.Practical implicationsThese findings suggest that a new media marketing strategy may be required that can provide a sufficient level of sensory stimulation to Millennials in order to increase their engagements in various use cases including entertainment, advertising and retail environments.Social implicationsDigital technology has so drastically reduced the costs of sharing and disseminating information, including music, that consumers can now easily use digital platforms to access a wide selection of music at minimal cost. The structure of the current music market reflects the decentralized nature of the online distribution network such that artists from all over the world now have equal access to billions of members of the global music audience.Originality/valueThis study confirms the importance of understanding target customer’s sensory experiences would grow in determining the success of digital contents and marketing.
Developing evaluation of signage for people with dementia
Authors: Gresham Meredith , Taylor Liz , Keyes Sarah , Wilkinson Heather , McIntosh Danielle , Cunningham Colm 
Published: August 2019

PurposeThe purpose of this paper is to to describe development of a framework for use in the evaluation of the effectiveness of signage to assist people with dementia.Design/methodology/approachThe study consisted of two parts. Workshops held in both Sydney and Edinburgh using “world café” methodology with 28 knowledgeable participants produced a pool of statements. These were subsequently used in a three-round Delphi process administered to 38 participants in order to generate ideas and develop consensus content for a signage evaluation framework.FindingsThis process resulted in a framework consisting of Delphi statements which had a 70 per cent level of agreement and a series of prompt questions. Both intrinsic factors and wider environmental, extrinsic factors in signage for wayfinding were identified.Research limitations/implicationsLimitations of this study were the small number of participants, including only four people with dementia, and the unresolved problems inherent in designing signage that may simultaneously be universally relevant and readily understood, yet meets the idiosyncratic needs of each individual living with dementia.Originality/valueAs there is little prior work in this field concerning signage use by people with dementia, this framework provides an original preliminary tool that may be used in further research on evaluating signage effectiveness. In designing signage and testing its effectiveness, it was concluded that the direct involvement of people with dementia is essential.
Complex state ownership, competition, and firm performance – Russian evidence
Authors: Liljeblom Eva , Maury Benjamin , Hörhammer Alexander 
Published: August 2019

PurposeState ownership has been common especially in industries with restricted competition. In Russia, state-controlled firms represent around 41 percent of the market value of all listed firms (Deloitte, 2015). Yet, there is a significant gap in the literature regarding the effects of various forms of government control in listed firms. The purpose of this paper is to fill this gap by exploring the impact of the complexity of state ownership and competition on the performance of Russian listed firms.Design/methodology/approachThe sample consists of data for 72 firms (360 firm-years) in the Russian MOEX broad market index during 2011–2015. The complexity of state ownership is captured by studying forms of state control including majority/minority, direct/indirect, federal/regional, mixed structures and golden shares.FindingsThe authors find significant differences in performance relating to different forms of state ownership. State control is negatively related to firm valuation and the sales/employees ratio. Performance is weakest when state ownership takes the form minority, regional or direct ownership. State control through golden shares typically outperforms other state-controlled firms. The authors find indications of employment prioritization beyond the economical optimum. In addition, the relation between state ownership and profitability becomes positive in sectors where state firms appear to enjoy lower competition.Originality/valueWhile the effects of state ownership have been studied on many markets, there is a lack of studies on the effects of different forms, or the complexity, of state ownership beyond direct and indirect ownership. The authors contribute to the literature on the performance effects of state ownership by studying a multitude of forms of governmental ownership as well as the role of competition in Russia. Especially the profitability of state-controlled firms is significantly affected by industry characteristics. Implications of the results are discussed both from firm and policy maker perspectives.
Internationalization paths of fruit export companies from emerging economies
Authors: Losilla Luis Vinicio , Engler Alejandra , Otter Verena 
Published: August 2019

PurposeThe purpose of this paper is to develop and apply a framework that examines the dynamics of internationalization strategies employed by export companies in the agricultural sector of emerging economies over time, with a focus on the locus of destination markets of the Chilean fruit sector. Thus, the objective is to identify conceptual and empirical deviations from existing research on export firms participating in non-agricultural sectors of industrialized countries.Design/methodology/approachThe matrix of multi-nationality developed by Aggarwalet al.(2011) is extended by incorporating the firm category of “host region” and the dimensions scale and time. This framework is utilized to classify 233 Chilean fresh fruit exporters according to their internationalization strategies based on a geographical distribution of their exports. A uni- and bivariate longitudinal analysis is conducted over a seven-year period (2009–2015) to explore the dynamics of this internationalization process.FindingsA significant number (12.75 percent) of firms classified as “host regional” are identified, and thus a clear difference in internationalization strategies when compared to non-agricultural sectors in industrialized countries. Simultaneously, similarities in these sectors can be found. Most firms are “transregionally” (65.12 percent) or “globally” oriented (16.06 percent), mainly following a linear internationalization path when considering the number of export markets. But there is also evidence of “born-global” firms, which mainly follow non-linear internationalization paths in more geographically and psychically distant markets.Research limitations/implicationsThe extended framework developed in this research can be applied to future studies, particularly in the case of economies where a significant proportion of firms are predominantly focusing their export strategies on one single international market. Since this study focuses on one national sector as a prime example, further studies on other countries and sectors may provide additional evidence of its generalizability.Practical implicationsBased on the findings, concrete measures have been suggested to aid Chilean policy makers in implementing evidence-based economic policies, as well as Chilean public trade organizations and private export associations in the fruit sector, in relation to services such as training, strategy consulting and trade network development that they provide to export firms.Originality/valueThe study contributes to the existing literature by introducing the firm category “host regional” into the matrix of multi-nationality, and empirically verifies its existence among agricultural export firms in emerging economies. Furthermore, it also shows that even when it might result counterintuitive, firms from the agricultural sector share similarities in internationalization strategies with firms from industrial sectors.
Retaining the social goal: role of path creation in for-profit social enterprises
Authors: Sarma Sushanta Kumar 
Published: August 2019

Purpose
The purpose of this paper is to understand how social enterprises (SEs) sustain social focus as they shift their legal format from nonprofit to for-profit. The investigation is driven by the understanding that historical persistence of organizational action can influence the sustenance of social focus.

Design/methodology/approach
The paper uses a case study approach and traces the commercialization process of two microfinance organization from India. The data come from interviews and archival documents spanning across the biography of the selected organizations. The constitutive elements of the commercialization process are identified by using the lens of path creation.

Findings
Evidence suggests that the framing of purpose for microfinance as empowerment of women formed the triggering event to path creation. The organizations retained the focus on social goal by adopting a community centered delivery model of self-help groups. The organizational practices adopted after commercialization helped these organization to address the issues of drift actively.

Research limitations/implications
The paper suggests that framing of organizational purpose can play a crucial role in sustaining hybrid character in SEs. It reinforces earlier findings that stakeholders can exert significant influence in balancing social and commercial goal. The aspiration to be identified as a pro-community organization is another critical driver in sustaining social focus. Finally, for SEs to sustain their social focus, proactive engagement with the community should become an integral part of organizational practices.

Originality/value
The paper explores the constitutive elements of path creation and demonstrates the sustenance of social focus through three stages of organizational path development. It also offers insights into the literature on historical imprinting by exploring the internal process through which imprinting is sustained and amplified and by presenting sources and outcome of imprinting.
A method to align functionalities of a manufacturing execution system with competitive priorities
Authors: Sellitto Miguel Afonso , Vargas Elisandro João de 
Published: August 2019

Purpose
The purpose of this paper is to align the implementation of the manufacturing execution systems (MES) functionalities with the manufacturing competitive dimensions of two companies of the metal-mechanics industry.

Design/methodology/approach
The research object comprises two Brazilian manufacturers that use MES. The research method is quantitative modeling. A literature review organized 24 functionalities of MES. The study prioritized the functionalities and the manufacturing competitive dimensions, evaluated the contribution and the quality of the implementation and classified the functionalities.

Findings
Two functionalities in the first and five in the second case have high contribution and quality. Three functionalities in the first and six in the second case have low contribution and quality. The first group should be more explored and updated. The second should be discontinued. The rest has intermediate graduations and should be studied case-by-case. The main contribution of the paper is the method, which can be replicated in other applications in the same or in other industries.

Research limitations/implications
The study relies on two case studies of the same industry. Further research shall encompass the entire industry and cases in industries others than the metal-mechanics.

Practical implications
MES users and vendors may benefit from the study as they can apply the method to align the implementation with the manufacturing strategy and therefore enhance the effectivity of the system.

Originality/value
The authors did not find a study that associates the performance or the contribution of the MES functionalities to the competitive priorities with the quality, integrity or consistency of the implementation.