bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening key lncRNAs for human rectal adenocarcinoma based on lncRNA‐mRNA functional synergistic network
Authors: Zhu Xiongwen , Wang Dongguo , Lin Qianyuan , Wu Guiyang , Yuan Shichao , Ye Fubo , Fan Qinghao 
Published: May 2019

AbstractBackgroundRectal adenocarcinoma (READ) is one of the deadliest malignancies, and the molecular mechanisms underlying the initiation and development of READ remain largely unknown. In this study, we aimed to find key long noncoding RNAs (lncRNAs) and mRNAs in READ by RNA sequencing.MethodsRNA sequencing was performed to identify differentially expressed mRNAs (DEmRNAs) and lncRNAs (DElncRNAs) between READ and normal tissue. READ‐specific protein‐protein interaction (PPI), DElncRNA‐DEmRNA coexpression, and DElncRNA‐nearby DEmRNA interaction networks were constructed. DEmRNAs and DEmRNAs coexpressed with DElncRNAs were functionally annotated.ResultsA total of 2113 DEmRNAs and 150 DElncRNAs between READ and normal tissue were identified. The PPI network identified several hub proteins, including CDK1, AURKB, CDC6, FOXQ1, NUF2, and TOP2A. The DElncRNA‐DEmRNA coexpression and DElncRNA‐nearby DEmRNA interaction networks identified some hub lncRNAs, including CCAT1, LOC105374879, GAS5, and B3GALT5‐AS1. The colorectal cancer pathway, the intestinal immune network for IgA production and the p53 signaling pathway were three pathways significantly enriched in DEmRNAs and DEmRNAs coexpressed with DElncRNAs. MSH6 coexpressed with two DElncRNAs (LOC105374879 and CASC15) and BCL2 coexpressed with B3GALT5‐AS1 were significantly enriched in the colorectal cancer signaling pathway. TNFRSF17 coexpressed with B3GALT5‐AS1 was enriched in the intestinal immune network for IgA production. CCNB2 coexpressed with LOC105374879 was enriched in the p53 signaling pathway.ConclusionA total of four DEmRNAs (MSH6, BCL2, TNFRSF17, and CCNB2) and three DElncRNAs (LOC105374879, CASC15, and B3GALT5‐AS1) may be involved in the pathogenesis of READ; this data may contribute to understanding the mechanisms of READ and the development of therapeutic strategies for READ.
Optimal close‐up views for precise 3D manipulation
Authors: Choi Myung Geol , Lee Ji Hye , Ha Wansu , Lee Kang Hoon 
Published: May 2019

AbstractDirect manipulation can be used as an intuitive interface for spatial interaction with multiple objects in virtual environments. However, users often have difficulties in precisely manipulating objects in the 3D space; this is mainly because the user is not provided with enough visual information to sufficiently recognize the spatial relationships among objects. We present a new method for improving the preciseness of object manipulation in the 3D space by providing the user with an auxiliary view that is consistently retargeted to closely highlight the spatial relationship between the manipulated object and the object nearest to it. Both the target and the position of the auxiliary camera are computed efficiently using the bisector surface of the two involved objects. The viewing target is set to the center of the closest area between the two objects, and the camera position is optimized under an objective function primarily measuring the visual clarity of the auxiliary view. We demonstrate the usefulness of our method through various experiments based on practical usage scenarios.
New Organometallic Tetraphenylethylene⋅Iridium(III) Complexes with Antineoplastic Activity
Authors: Liu Xicheng , He Xiangdong , Zhang Xiaojing , Wang Yongling , Liu Jiaying , Hao Xiujuan , Zhang Yue , Yuan Xiang‐Ai , Tian Laijin , Liu Zhe 
Published: May 2019

AbstractIridium(III) complexes have attracted more and more attention in the past few years because of their potential antineoplastic activity. In this study, four IrIII complexes of the types [(η5‐Cpx)Ir(N^N)Cl]PF6 (complexes 1 and 2) and [Ir(Phpy)2(N^N)]PF6 (complexes 3 and 4) have been synthesized and characterized. They exhibit potential antineoplastic activity towards A549 cells, especially in the case of complex 1 [IC50=(3.56±0.5) μm], which was nearly six times as effective as cisplatin [(21.31±1.7) μm]. Additionally, these complexes show some selectivity towards cancer cells over normal cells. They could be transported by serum albumin (binding constants were changed from 0.37×105 to 81.71×105 m−1). IrIII complexes 1 and 2 could catalyze the transformation of nicotinamide adenine dinucleotide reduced form (NADH) into NAD+ (turnover numbers 43.2, 11.9] and induce the accumulation of reactive oxygen species, thus confirming their antineoplastic mechanism of oxidation, whereas the cyclometalated complexes 3 and 4 were able to target the lysosome [Pearson co‐localization coefficient (PCC)=0.73], cause lysosomal damage, and induce apoptosis. Understanding the mechanism of action would help further structure–activity optimization on these IrIII complexes as emerging cancer therapeutics.
Poly(sodium 4‐styrenesulfonate) Assisted Room‐Temperature Synthesis for the Mass Production of Bismuth Oxychloride Ultrathin Nanoplates with Enhanced Photocatalytic Activity
Authors: Zeeshan Shahid Malik , Wei Yunwei , Wang Junnuan , Chen Guozhu , Gao Daowei , Ye Chen , Sun Yiqiang , Liu Guang‐Ning , Li Cuncheng 
Published: May 2019

AbstractBismuth oxychloride ultrathin nanoplates (BiOCl‐UTNs) are highly active, but their preparation are limited to closed‐vessel hydrothermal and solvothermal techniques at high temperatures (110–180 °C). Here we report a straightforward poly(sodium 4‐styrenesulfonate) (PSS)‐mediated route for the large‐scale synthesis of BiOCl‐UTNs at room‐temperature. In an open vessel, 6.15 g of BiOCl‐UTNs with 3–5 nm thickness, and planar dimensions of 30–50 nm were produced. The strong electrostatic interaction between PSS and [Bi2O2]2+ layers inhibited the growth rate of BiOCl nanoplates along <001> direction, and Na+ ions governed the electrolyte sedimentation to produce BiOCl‐UTNs. The resulting BiOCl‐UTNs exhibited high photocatalytic activity for the degradation of antibiotics and organic dyes because of their large specific surface area, increased light absorption ability, and fast separation and transfer efficiency of the photoexcited charge carriers.
Some like it dry: Water restriction overrides heterogametic sex determination in two reptiles
Authors: Dupoué Andréaz , Lourdais Olivier , Meylan Sandrine , Brischoux François , Angelier Frédéric , Rozen‐Rechels David , Marcangeli Yoan , Decencière Béatriz , Agostini Simon , Le Galliard Jean‐François 
Published: May 2019

AbstractThe evolution of sex determination is complex and yet crucial in our understanding of population stability. In ectotherms, sex determination involves a variety of mechanisms including genetic determination (GSD), environment determination (ESD), but also interactions between the two via sex reversal. In this study, we investigated whether water deprivation during pregnancy could override GSD in two heterogametic squamate reptiles. We demonstrated that water restriction in early gestation induced a male‐biased secondary sex ratio in both species, which could be explained by water sex reversal as the more likely mechanism. We further monitored some long‐term fitness estimates of offspring, which suggested that water sex determination (WSD) represented a compensatory strategy producing the rarest sex according to Fisher's assumptions of frequency‐dependent selection models. This study provides new insights into sex determination modes and calls for a general investigation of mechanisms behind WSD and to examine the evolutionary implications.OPEN RESEARCH BADGES
This article has earned an Open Data Badge for making publicly available the digitally‐shareable data necessary to reproduce the reported results. The data is available at https://doi.org/10.5061/dryad.mv06pv1.
Enhancing a Novel Robust Multicomposite Materials Platform for Glucose Biosensors
Authors: Moonla Chochanon , Nontapha Chantarattana , Ouiram Tik , Preechaworapun Anchana , Tangkuaram Tanin 
Published: May 2019

AbstractAn effective, stable enzymatic glucose biosensor was fabricated on a glassy carbon electrode (GCE) surface using simple multicomposite materials (MCM): a solution of prepared poly(diallyldimethylammonium chloride)‐capped gold nanoparticles‐nickel ferrite particles‐carbon nanotubes‐chitosan (PDDA‐AuNPs‐NiFe2O4‐CNTs‐CHIT), electropolymerization of poly(o‐phenylenediamine) (PoPD) and immobilization of glucose oxidase (GOx). Biocompatibility and synergy of the MCM enhanced the immobilization and the reaction of GOx and as well as the electron transfer from an oxidation reaction of hydrogen peroxide in the system. The NiFe2O4 was synthesized by co‐precipitation and calcined at 700 °C. Characterization was carried out by field emission scanning electron microscopy (FE‐SEM), energy‐dispersive X‐ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR) and X‐ray diffraction (XRD) which presented both tetrahedral and octahedral metal stretching with a cubic NiFe2O4 crystal phase. The GOx/PoPD/MCM/GCE yielded a 0.77 s−1 charge transfer rate constant (Ks), a 2.28×10−6 cm2 s−1 diffusion coefficient value (D), a 0.21 mm2 electroactive surface area (Ae) and a 1.93×10−8 mol cm−2 surface concentration (
) as determined by cyclic voltammetry. The modified electrode showed a durable operation time (n=97, more than 50 % I), repeatability (%RSD=0.38, n=10), reproducibility (%RSD=1.60, n=10), high sensitivity (853.07 μA mM−1 cm−2), selectivity without effects of electroactive species (aspirin, uric acid, caffeine, cholesterol, ascorbic acid and dopamine) and two linear ranges from 0.5 to 10 μM (R2=0.998) and 10 to 15,000 μM (R2=0.991) with a low detection limit (0.35 μM, S/N=3). Its Michaelis‐Menten constant (Km) was calculated as 93.51 μM with 46.30 μA maximum current (Imax). This proposed simple method was successfully applied for glucose determination in human blood samples.
A Simple and Sensitive Square Wave Stripping Pathway for the Analysis of Desmedipham Herbicide by Modified Carbon Paste Electrode Based on Hematite (α‐Fe2O3 Nanoparticles)
Authors: Demir Ersin 
Published: May 2019

AbstractIn the present study, desmedipham, used as an herbicide to control broad leaf weed in commonly sugar beet crops, was analyzed at the first time by cyclic voltammetry (CV) and square wave stripping voltammetry (SWSV) with the modified carbon paste electrode based on hematite nanoparticles (α‐Fe2O3−CPE). The modified α‐Fe2O3−CPE prepared by hematite (α‐Fe2O3 nanoparticles), which is very sensitive to carbon paste electrode (CPE) and glassy carbon electrode (GCE). Morphology of electrode surface detailed by scanning electron microscopy (SEM) and energy dispersive X‐ray analysis (EDX). The oxidation of desmedipham created irreversible well‐done two peaks at nearly +1.1 and +1.3 V. In order to obtain the best calibration graph, various important parameters such as pH, accumulation time, puls amplitude and frequency etc. were investigated. Under the optimum conditions, electrochemical behavior of desmedipham presented two linear working ranges at 0.15–1.20 mg/L and 1.20–4.50 mg/L. The limits of detection (LOD) for the desmedipham were calculated as 41.00 and 50.00 μg/L for the peak I and II, respectively. Furthermore, desmedipham was analyzed at high recovery in the presence of rizolex, fluometuron, teflubenzuron and some heavy metal ions. Consequently, the developed SWSV was successfully applied to evaluate desmedipham in spiked commercial strawberry juices at recoveries of 96.00–104.00 % with satisfactory relative standard deviations.
Disposable Biosensor Based on Amidase/CeO2/GNR Modified Inkjet‐printed CNT Electrodes‐droplet Based Paracetamol Detection in Biological Fluids for “Point‐of‐care” Applications
Authors: Stanković Dalibor M. , Ognjanović Miloš , Jović Milica , Cuplić Valentina , Lesch Andreas , Girault Hubert H. , Gavrović Jankulović Marija , Antić Bratislav 
Published: May 2019

AbstractA disposable acetaminophen biosensor based on inkjet‐printed CNT electrodes (IJPCNT) modified with amidase/cerium dioxide@graphene nanoribbons composite was developed (ACeO2@GNR/IJPCNT). The enzyme amidase A was used for the first time as a recognition element. Inkjet‐printed CNT electrodes served as a basis for the construction of a biosensor that enables droplet detection using 5 μL sample volume. The biosensor showed high selectivity, sensitivity, a low detection limit of 0.18 μM and a wide working linear range from 1 to 100 μM. The proposed approach allows fast and reliable detection of acetaminophen in biological fluids with negligible matrix effect and remarkable reproducibility.
Novel Multiplexed Biosensor System for the Determination of Lactate and Pyruvate in Blood Serum
Authors: Kucherenko Ivan S. , Soldatkin Oleksandr O. , Topolnikova Yaroslava V. , Dzyadevych Sergei V. , Soldatkin Alexei P. 
Published: May 2019

AbstractMultiplexing is one of the main current trends in biosensors, especially important for clinical diagnosis. However, simultaneous determination of several substances in one sample is often difficult due to different performance and working conditions of separate biosensors. This work was aimed at the development of a multiplexed biosensor system for the determination of lactate and pyruvate concentrations in liquid samples (i. e., blood serum). The system consisted of two amperometric biosensors based on lactate oxidase and pyruvate oxidase, which worked simultaneously in a single measuring cell. Conditions for the biosensor system work were selected. Linear range of lactate determination was 5–1000 μM, pyruvate – 10–5000 μM. Steady‐state response time was 30 s and 50 s for the lactate and pyruvate biosensors, respectively. After 2 weeks of storage biosensor responses decreased to 95 % (lactate biosensor) or 82 % (pyruvate biosensor) of the initial value. A scheme of analysis of the concentrations of lactate and pyruvate in human blood serum was proposed. The lactate and pyruvate concentrations as well as their ratio in human blood serum samples were determined and compared with the control method. The proposed biosensor system is suitable for the rapid detection of lactate, pyruvate and their ratio and can be used for clinical diagnosis, e. g., evaluation of the reasons of lactic acidosis and prognosis of patient's recovery.
Glyoxal cross‐linking of solubilized extracellular matrix to produce highly porous, elastic, and chondro‐permissive scaffolds for orthopedic tissue engineering
Authors: Browe David C. , Mahon Olwyn R. , Díaz‐Payno Pedro J. , Cassidy Nina , Dudurych Ivan , Dunne Aisling , Buckley Conor T. , Kelly Daniel J. 
Published: May 2019

AbstractExtracellular matrix (ECM)‐derived implants hold great promise for tissue repair, but new strategies are required to produce efficiently decellularized scaffolds with the necessary porosity and mechanical properties to facilitate regeneration. In this study, we demonstrate that it is possible to produce highly porous, elastic, articular cartilage (AC) ECM‐derived scaffolds that are efficiently decellularized, nonimmunogenic, and chondro‐permissive. Pepsin solubilized porcine AC was cross‐linked with glyoxal, lyophilized and then subjected to dehydrothermal treatment. The resulting scaffolds were predominantly collagenous in nature, with the majority of sulphated glycosaminoglycan (sGAG) and DNA removed during scaffold fabrication. Four scaffold variants were produced to examine the effect of both ECM (10 or 20 mg/mL) and glyoxal (5 or 10 mM) concentration on the mechanical and biological properties of the resulting construct. When seeded with human infrapatellar fat pad‐derived stromal cells, the scaffolds with the lowest concentration of both ECM and glyoxal were found to promote the development of a more hyaline‐like cartilage tissue, as evident by increased sGAG and type II collagen deposition. Furthermore, when cultured in the presence of human macrophages, it was found that these ECM‐derived scaffolds did not induce the production of key proinflammatory cytokines, which is critical to success of an implantable biomaterial. Together these findings demonstrate that the novel combination of solubilized AC ECM and glyoxal crosslinking can be used to produce highly porous scaffolds that are sufficiently decellularized, highly elastic, chondro‐permissive and do not illicit a detrimental immune response when cultured in the presence of human macrophages.
GATA4 screening in Iranian patients of various ethnicities affected with congenital heart disease: Co‐occurrence of a novel de novo translocation (5;7) and a likely pathogenic heterozygous GATA4 mutation in a family with autosomal dominant congenital heart disease
Authors: Kalayinia Samira , Maleki Majid , Rokni‐Zadeh Hassan , Changi‐Ashtiani Majid , Ahangar Hassan , Biglari Alireza , Shahani Tina , Mahdieh Nejat 
Published: May 2019

AbstractBackgroundCongenital heart disease (CHD) is the most common birth defect and a major health problem around the world. However, its exact etiology has remained unclear. Among various genetic contributing factors, GATA4 transcription factor plays a significant role in the CHD pathogenesis. In this study, GATA4 coding sequence was screened in Iranian patients of various ethnicities.MethodsSixty six individuals with familial CHD referred to our center were recruited in this study. After receiving written informed consent from each individual or their parents, chromosomal analyses and GATA4 variant screening were performed. Pathogenicity of the suspected variants was evaluated using available online software tools: CADD, Mutation Taster, SIFT, and PolyPhen‐2.ResultsA total of twelve GATA4 variants were detected including five intronic, 2 exonic and 3 polymorphisms as well as 2 missense mutations, the c.1220C>A and c.1309G>A. Unlike the c.1220C>A, the likely pathogenic heterozygous c.1309G>A has not been previously associated with any phenotype. Here, we not only report, for the first time, a c.1309G>A‐related CHD, but also report a novel de novo balanced translocation, 46,XY,t(5;7)(qter13;qter11), in the same patient which may have influenced the disease severity.ConclusionFrom screening GATA4 sequence in 66 Iranian patients of various ethnicities, we conclude that cytogenetic analysis and PCR‐direct sequencing of different candidate genes may not be the best approach for genetic diagnosis in CHD. Applying novel approaches such as next‐generation sequencing (NGS) may provide a better understating of genetic contributing factors in CHD patients for whom conventional methods could not reveal any genetic causative factor.
Reprocessable Polymer Networks Designed with Hydroxyurethane Dynamic Cross‐links: Effect of Backbone Structure on Network Morphology, Phase Segregation, and Property Recovery
Authors: Chen Xi , Li Lingqiao , Wei Tong , Torkelson John M. 
Published: May 2019

AbstractPolybutadiene (PB), poly(tetramethylene oxide) (PTMO), and polydimethylsiloxane (PDMS) networks are synthesized using relatively low wt% of dynamic hydroxyurethane cross‐links, and recovery of cross‐link density, network morphology, and properties are investigated as a function of reprocessing. PB and PTMO networks exhibit full recovery of rubbery plateau modulus, and thus cross‐link density, and tensile properties after multiple melt‐state recycling steps. PDMS networks exhibit a small loss in rubbery plateau modulus with reprocessing. Small‐angle X‐ray scattering reveals nanophase separation in PB and PDMS networks. Although PTMO networks are not nanophase separated, cold crystallization is observed, with crystallinity increasing after reprocessing because of chain alignment. This work establishes the effective use of hydroxyurethane cross‐links toward full property recovery in different networks and provides insights on the design of reprocessable networks with distinctive morphology and sustainability.
MicroRNA‐210 regulates human trophoblast cell line HTR‐8/SVneo function by attenuating Notch1 expression: Implications for the role of microRNA‐210 in pre‐eclampsia
Authors: Wang Rongli , Liu Weifang , Liu Xiaoping , Liu Xiaoxia , Tao Hui , Wu Di , Zhao Yin , Zou Li 
Published: May 2019

AbstractSuccessful pregnancy depends on the precise regulation of extravillous trophoblast cell invasion ability. MicroRNA‐210‐3p (miR‐210), which is increased in the placenta of pre‐eclampsia. Furthermore, miR‐210 could inhibit trophoblasts invasion and might act as a serum biomarker for pre‐eclampsia. Previous studies have demonstrated that miR‐210 regulates HUVEC (human umbilical vein endothelial cell)‐mediated angiogenesis by regulating the NOTCH1 signaling pathway. Studies by our group have previously identified that NOTCH1 plays a positive role in regulating trophoblast functions. However, the miR‐210/NOTCH1 signaling pathway in the regulation of trophoblasts and pre‐eclampsia has not been characterized. Therefore, this study was conducted to investigate the role of miR‐210 and its relationship with NOTCH1 in trophoblasts. We first examined the expression levels of miR‐210 and NOTCH1 in pre‐eclamptic and normals placentas. Next, the expression and location of miR‐210 and NOTCH1 in the first‐trimester villi, maternal decidua, and placenta of late pregnancy were shown via in situ hybridization and immunohistochemistry. The trophoblast cell line HTR‐8/SVneo was used to investigate the effects of miR‐210 on the expression of NOTCH1 and cell bioactivity by upregulation and downregulation strategies. The results showed that miR‐210 expression was increased, whereas NOTCH1 expression was decreased in pre‐eclamptic placenta compared with controls. Upregulation of miR‐210 decreased NOTCH1 expression, impaired HTR‐8/SVneo proliferation, migration, invasion, and tube‐like formation capabilities, and promoted apoptosis. In contrast, downregulation of miR‐210 resulted in the opposite effects. These findings suggested that miR‐210 might act as a contributor to trophoblast dysfunction by attenuating NOTCH1 expression.
The implication of single‐nucleotide polymorphisms in ovarian hyperstimulation syndrome
Authors: Ghaderian Sayyed Mohammad Hossein , Akbarzadeh Reza , Mohajerani Fatemeh , Khodaii Zohreh , Salehpour Saghar 
Published: May 2019

AbstractOvarian hyperstimulation syndrome (OHSS) is an undesirable complication in the course of ovarian stimulation. This kind of stimulation is aimed at acquiring a sufficient number of high‐quality oocytes in in vitro fertilization (IVF). Whereas the predisposition to OHSS could be impacted by genetic polymorphisms in susceptible genes, the present study has been jointly conducted with an Iranian cohort to scrutinize its relevant implication. Genomic DNA was extracted from blood samples of patients with a normal ovarian response (NOR) or with OHSS. Samples were analyzed to detect polymorphisms MTHFR rs1801131, MTHFR rs1801133, AMHR2 rs2002555, LHCGR rs2293275, PGR rs10895068, and SERPINE1 rs1799889. Variations of MTHFR, AMHR2, LHCGR, and PGR genes were significantly associated with the developing OHSS. After correction for multiple analysis, this difference was not evident for PGR genotypes. The polymorphic alleles of MTHFR (rs1801131 C‐allele and rs1801133 T‐allele), AMHR2 (rs2002555 G‐allele), and LHCGR (rs2293275 G‐allele) were significantly more prevalent among patients with OHSS compared to those in the NOR group. In contrast, the minor allele of PGR single‐nucleotide polymorphism (SNP) (rs10895068, A‐allele) was more prominent among patients with a NOR than those with OHSS. No significant difference was observed in genotypes or alleles of SERPINE1 rs1799889. The observations indicated that the minor alleles of MTHFR, AMHR2, and LHCGR genes could be considered an independent risk factor in susceptibility to OHSS. Nevertheless, polymorphic allele in the PGR rs10895068 SNP contributes to preventing OHSS occurrence. Therefore, it can be argued that these genes have a significant impact on OHSS.