bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical reprogramming enhances homology-directed genome editing in zebrafish embryos
Authors: Aksoy Yagiz A. , Nguyen David T. , Chow Sharron , Chung Roger S. , Guillemin Gilles J. , Cole Nicholas J. , Hesselson Daniel 
Published: May 2019

AbstractPrecise genome editing is limited by the inefficiency of homology-directed repair (HDR) compared to the non-homologous end-joining (NHEJ) of double strand breaks (DSBs). The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 system generates precise, locus-specific DSBs that can serve as substrates for HDR. We developed an in vivo visual reporter assay to quantify HDR-mediated events at single-cell resolution in zebrafish and used this system to identify small-molecule modulators that shift the DNA repair equilibrium in favor of HDR. By further optimizing the reaction environment and repair template, we achieved dramatic enhancement of HDR-mediated repair efficiency in zebrafish. Accordingly, under optimized conditions, inhibition of NHEJ with NU7441 enhanced HDR-mediated repair up to 13.4-fold. Importantly, we demonstrate that the increase in somatic HDR events correlates directly with germline transmission, permitting the efficient recovery of large seamlessly integrated DNA fragments in zebrafish.
Association of miR-146a, miR-149 and miR-196a2 polymorphisms with neuroblastoma risk in Eastern Chinese population: a three-center case–control study
Authors: Zhou Chunlei , Tang Yingzi , Zhu Jinhong , He Lili , Li Jinghang , Wang Yizhen , Zhou Haixia , He Jing , Wu Haiyan 
Published: May 2019

Abstract
Neuroblastoma is one of the most common malignancy in childhood, which originates from the developing sympathetic nervous system. Single nucleotide polymorphisms (SNPs) in primary miRNA (pri-miRNA) have shown to associate with cancer susceptibility, including neuroblastoma. Three precursor miRNA (pre-miRNA) SNPs (pre-miR-146a rs2910164, pre-miR-149 rs2292832 and pre-miR-196a2 rs11614913) were found to contribute to pathogenesis of various diseases. Here, to evaluate the association among these three pre-miRNA SNPs and neuroblastoma susceptibility in Eastern Chinese children, we carried out a three-center case–control study involving 312 neuroblastoma cases and 762 healthy controls. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the association of these three polymorphisms with neuroblastoma risk. However, no significant association was observed among these three SNPs and neuroblastoma susceptibility, in either overall or subgroups analysis by tumor sites, gender and age. Further larger studies consisting of diverse ethnic populations are required to clarify the associations among these three pre-miRNAs polymorphisms and neuroblastoma risk.
Nicotine promotes the development of non-small cell lung cancer through activating LINC00460 and PI3K/Akt signaling
Authors: Zhao Hongying , Wang Yu , Ren Xiubao 
Published: May 2019

Abstract
Objective: Nicotine, the main ingredient in tobacco, is identified to facilitate tumorigenesis and accelerate metastasis in tumor. Studies in recent years have reported that long intergenic non-protein coding RNA 460 (LINC00460) is strongly associated with lung cancer poor prognosis and nicotine dependence. Nonetheless, it is unclear whether nicotine promotes the development of lung cancer through activation of LINC00460. Methods: We determined that LINC00460 expression in lung cancer tissues and the prognosis in patients with non-small cell lung carcinoma (NSCLC) using Gene Expression Profiling Interactive Analysis (GEPIA) website and The Cancer Genome Atlas (TCGA) database. Through in vitro experiments, we studied the effects of nicotine on LINC00460 in NSCLC cells lines using Cell Counting Kit-8 (CCK-8), transwell test, flow cytometry, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Western blot assays. Results: We identified the significant up-regulated expression level of LINC00460 in NSCLC tissues and cell lines, especially, the negative correlation of LINC00460 expression level with overall survival (OS). In in vitro experiments, LINC00460 was overexpressed in NSCLC cell lines under nicotine stimulation. Nicotine could relieve the effect of LINC00460 knockdown on NSCLC cell proliferation, migration and apoptosis. The same influence was observed on PI3K/Akt signaling pathway. Conclusions: In summary, this is the first time to examine the potential roles of LINC00460 in lung cancer cell proliferation, migration and apoptosis induced by nicotine. This may help to develop novel therapeutic strategies for the prevention and treatment of metastatic tumors from cigarette smoke-caused lung cancer by blocking the nicotine-activated LINC00460 pathway.
Trusted FPGA‐based transport traffic inject, impersonate (I2) attacks beaconing in the Internet of Vehicles
Authors: Appathurai Ahilan , Manogaran Gunasekaran , Chilamkurti Naveen 
Published: May 2019

Internet of Things assisted vehicular ad‐hoc network VANET (I‐VANET) is one of the preferred technologies to provide fast and reliable communication in smart transportation systems. With the goal of preserving security, I‐VANET is characterised by field‐programmable gate array‐based remote dynamic partial reconfiguration (RDPR) and security providing entity as a washable physical unclonable function (WPUF). In this specific work, an efficient WPUF‐based unknown authentication scheme is proposed to identify and avoid malicious vehicles entering into the I‐VANET. In addition to that, the proposed work provides a conditional tracing mechanism to hint the vehicles or roadside units that misuse the I‐VANET. This system reveals the malicious vehicles to efficiently provide conditional privacy through I‐VANET, by enabling a situation where the entities are unknown to each other until they depart from the network. Finally, I2 attacks were analysed from possible attacks that can emanate from the availability of RDPR at I‐VANET nodes, and WPUF circuit was used to prevent such attacks. The proposed WPUF‐based authentication scheme takes only around 120 ms for verifying 100 tokens but other conventional techniques take >900 ms for verifying 100 tokens.
Untersuchung der Akzeptanz und der Ablehnungsgründe indirekter Vergleiche in IQWiG-Nutzenbewertungen
Authors: Werner Sebastian , Lechterbeck Lisa , Rasch Andrej , Merkesdal Sonja , Ruof Jörg 
Published: May 2019

Zusammenfassung
Hintergrund/Fragestellung Seit dem Inkrafttreten des „Arzneimittelmarktneuordnungsgesetzes“ (AMNOG) 2011 bewertet der „Gemeinsame Bundesausschuss“ (GBA) unter regelhafter Einbindung des Instituts für Qualität und Wirtschaftlichkeit im Gesundheitswesen (IQWiG) den Zusatznutzen von Arzneimitteln mit neuen Wirkstoffen gegenüber einer zweckmäßigen Vergleichstherapie. Dieser bildet die Basis für Verhandlungen über den Erstattungsbetrag. Bei dieser Bewertung ist die Möglichkeit vorgesehen, Nachweise zum Zusatznutzen durch indirekte Vergleiche erbringen zu können, falls keine direkten Vergleichsstudien verfügbar sind. In IQWiG-Nutzenbewertungen, die für den GBA wichtige Entscheidungshilfen darstellen, werden diese jedoch häufig abgelehnt. Ziel dieser Untersuchung ist die systematische Überprüfung der Akzeptanz von vorgelegten indirekten Vergleichen in IQWiG-Nutzenbewertungen sowie der entsprechenden Ablehnungsgründe.
Methoden In Nutzendossiers vorgelegte indirekte Vergleiche sowie deren Akzeptanz bzw. Ablehnungsgründe in IQWiG-Nutzenbewertungen wurden im Zeitraum 01.2011 bis 10.2017 untersucht. Dabei wurden 6 Ablehnungskriterien systematisch geprüft, die sich an IQWiG-Anforderungen orientieren: (1) zweckmäßige Vergleichstherapie (ZVT), (2) Vollständigkeit der Studiendaten, (3) Studieneignung, (4) Studienähnlichkeit, (5) statistische Verfahren, (6) Homogenität/Konsistenz.
Ergebnisse In 62 AMNOG-Verfahren mit 52 Wirkstoffen bzw. Wirkstoffkombinationen wurden insgesamt 111 indirekte Vergleiche identifiziert. 94 % der indirekten Vergleiche wurden vom IQWiG nicht akzeptiert. Häufigster Ablehnungsgrund war die Studieneignung (47,7 %) gefolgt von Studienähnlichkeit (39,6 %) und Vollständigkeit der Studiendaten (38,7 %). Auch statistische Verfahren waren ein bedeutsamer Ablehnungsgrund (25,2 %), wobei es dabei fast ausschließlich zu einer Ablehnung von sog. „nicht-adjustierten“ indirekten Vergleich kam. Die ZVT (8,1 %) wie auch Homogenität/Konsistenz (2,7 %) waren seltene Ablehnungsgründe.
Schlussfolgerung Indirekte Vergleiche werden vom IQWiG in der Regel aufgrund eines ungeeigneten Studienpools (Studieneignung, -ähnlichkeit, -vollständigkeit) oder hoher Anforderungen an nicht-adjustierte indirekte Vergleiche abgelehnt. In Anlehnung an die Vorgehensweise der Cochrane Collaboration sollten realistische und pragmatische methodische Standards für die Durchführung von indirekten Vergleichen im Rahmen von AMNOG-Verfahren entwickelt werden. Leitfäden des IQWIG sowie methodisch tiefergehende Beratungsmöglichkeiten beim GBA sind erforderlich, um die entsprechenden Einreichungen der pU im Vorhinein zu optimieren.
Three-dimensional Cat Virtual Anatomy: Development of an Interactive Virtual Anatomical Software
Authors: Osorio-Echeverri Juan Sebastián , Orrego-Metaute Diana Alexandra , Murillo-Escobar Juan Pablo , Tamayo-Arango Lynda 
Published: May 2019

Background Three-dimensional (3D) virtual models are novel tools to teach veterinary anatomy.
Objective The aim of the present study was to create a 3D cat image software and a library of cross-sectional images.
Methods Modeling of the 3D cat organs and structures was done with Autodesk Maya, version 2017 (Autodesk Inc., San Rafael, California, USA) and ZBrush, version 4R7 (Pixologic, Los Angeles, CA, USA) software. In order to obtain the images for the library, three cadavers of adult cats were used, with the following techniques: 1) scanning by magnetic resonance imaging (MRI) at 3-mm intervals, 2) scanning by computed tomography (CT) at 2-mm intervals, and 3) photographing of 178 transverse cuts at 2.5-mm intervals from the frozen cadavers. Out of all the images, thirty images of each technique were selected. An interactive software was developed with the modeled 3D cat and the selected images using Unity, version 5.4 (Unity Technologies, San Francisco, CA, USA).
Results A virtual 3D cat model was obtained with 418 labeled structures of the skeletal, muscular, circulatory, nervous, respiratory, digestive, urinary, and integumentary systems. The virtual interface enables the manipulation of the 3D cat in all views and the visualization of the selected images in a chosen localization along the body of the cat. The library of images allows comparison among CT, MRI and photographs of transverse cuts.
Conclusions The software interface facilitates the access to the content for the user. Sectional images of the cat and of its body structures can be easily understood. This new 3D software of cat anatomy is another tool that can be used in teaching veterinary anatomy.
Gangliones volares de muñeca – señal externa de patología intraarticular
Authors: Cruz-Sánchez Marcos , Martínez-Andrade Cristóbal , Escudero-González Oscar , Castro-Alvarez María Concepción , Morell-Luque Juan Manuel , Castillo-García Félix 
Published: May 2019

Introducción La etiología y el manejo de los gangliones sigue en controversia pese a su elevada frecuencia. Concretamente el tratamiento del ganglión volar de muñeca se ha asociado a un mayor índice de recidiva y complicaciones comparado con el ganglión dorsal. En los últimos años, se ha desarrollado el tratamiento artroscópico con el fin de realizar un tratamiento menos invasivo y poder identificar una relación causal. El objetivo del estudio es determinar la presencia de lesiones intraarticulares asociadas al ganglión volar y si el tratamiento de ambas patologías puede ser una opción válida.
Material y Método Estudio longitudinal en el que se intervinieron de forma consecutiva 21 pacientes de ganglión volar de muñeca por vía artroscópica. Se recogieron los datos sociodemográficos, exploración física, pruebas complementarias, región predominante de dolor (EVA) y cuestionario DASH pre y postoperatorio. Se asoció el tratamiento de las lesiones intraarticulares en el mismo acto quirúrgico si existía correlación clínica con la exploración física inicial.
Resultados Todos los casos fueron intervenidos por vía artroscópica sin complicaciones intraoperatorias. Se observaron hallazgos artroscópicos en todos los casos siendo la mayoría lesiones del Fibrocartilago Triangular del Carpo (57,12%). Se obtuvo una mejoría en los parámetros de dolor y DASH postoperatorios siendo este último significativo en los casos de dolor cubital y dorsal asociado al ganglión (p < 0.02). Hubo 2 casos de recidiva 9,52% que requirieron reintervención.
Conclusiones Existe un gran número y variabilidad de lesiones intraarticulares asociadas al ganglión volar de muñeca. El tratamiento artroscópico es una herramienta versátil y útil ya que permite un tratamiento combinado. Se ha observado una mejora en los resultados postoperatorios con un escaso índice de complicaciones.
Los parámetros espermáticos funcionales como factor pronóstico en ICSI: Experiencia de la ciudad de Medellín
Authors: Calderón-Mendoza Luisa F. , López Lucero Castrillón , Vélez Giraldo Carlos Felipe , Isaza Álvarez Verónica , Cardona-Maya Walter D. 
Published: May 2019

Introducción La infertilidad por factor masculino afecta al 30% de las parejas infértiles y la evaluación seminal es crítica en las determinaciones que conllevan a un posible tratamiento con el fin de tener un resultado exitoso.
Objetivo El objetivo de este estudio fue evaluar la relación que existe entre los parámetros seminales convencionales y funcionales, con las tasas de fecundación, desarrollo embrionario y embarazo obtenidas después de inyección intracitoplasmática de espermatozoides (ICSI).
Métodos 36 muestras seminales de parejas que se sometieron a ICSI (18 usando oocitos propios y 18 de donante), fueron evaluadas de manera convencional, posteriormente se seleccionaron los espermatozoides, se realizó ICSI y una alícuota se utilizó para cuantificar las siguientes pruebas funcionales: potencial de membrana mitocondrial, integridad de la membrana, detección de especies reactivas de oxígeno e índice de fragmentación del ADN.
Resultados No se encontraron diferencias significativas en cuanto a los parámetros convencionales y funcionales en los dos grupos, como tampoco se encontró una relación significativa entre los parámetros evaluados y los resultados de ICSI. Sólo se observó que la tasa de embarazo fue mayor en el grupo de oocitos donados (p < 0,0001).
Conclusiones Los datos obtenidos en este estudio sugieren que no existe correlación entre los parámetros evaluados y los resultados de ICSI. Eso se debe probablemente, a que la selección de los espermatozoides tanto por gradientes de densidad como la posterior selección durante el procedimiento del ICSI, tiene un bajo poder predictivo sumado a la capacidad que tiene el oocito de reparar los daños presentes en el espermatozoide.
Stretched Earlobe Piercings Negatively Impact Casual Observer Perceptions
Authors: Fung Nicholas , Ishii Masaru , Huynh Pauline , Juarez Michelle , Bater Kristin , Darrach Halley , Papel Ira D. , Kontis Theda , Nellis Jason C. , Ishii Lisa 
Published: May 2019

AbstractPatients with stretched earlobes seek reconstruction to mitigate social stigma. To date, there have been no studies measuring the impact of stretched earlobe piercings on casual observer perceptions. One-hundred seventy-three casual observers were enrolled via public-access web sites. Participants were randomly shown frontal and profile views of six subjects with stretched earlobe piercings and four controls. Participants evaluated photos for first impressions using a survey containing choices regarding personal attributes. Latent class analysis was performed to categorize observer ratings. Analysis of variance (ANOVA), bootstrap analysis, and permutations testing were used to evaluate the relationship between perceived attractiveness, success, and approachability scoring and stretched earlobe status. Latent class analysis categorized responses into three classes: positive, negative, and neutral. Patients with stretched earlobe piercings were significantly less likely to be classified as positive by observers without body modifications (i.e., tattoos and piercings) in comparison to control photos (30.9 and 40.1%, p = 0.007) and more likely to be classified as negative (38.5 and 28.1%, p = 0.002). These changes were abolished when photos were evaluated by observers with body modifications (p > 0.05). ANOVA revealed that stretched earlobe piercings and observer body modification status have a significant effect on rated approachability (F [1,1726] = 4.08, p = 0.04) and successfulness (F[1,1726] = 9.67, p = 0.002; F [1,1726] = 70.33, p < 0.0005). No significance was found for rated attractiveness (p > 0.05). Patients with stretched earlobe piercings were more likely to be classified as having negative affect display and being less approachable and successful compared with controls when evaluated by observers without body modifications. This effect was abolished when photos were evaluated by observers with body modifications. These findings validate patient motivations for seeking stretched earlobe repair.
Benchmarking high fidelity single-shot readout of semiconductor qubits
Authors: Keith D , Gorman S K , Kranz L , He Y , Keizer J G , Broome M A , Simmons M Y 
Published: May 2019

Abstract
Determination of qubit initialisation and measurement fidelity is important for the overall performance of a quantum computer. However, the method by which it is calculated in semiconductor qubits varies between experiments. In this paper we present a full theoretical analysis of electronic single-shot readout and describe critical parameters to achieve high fidelity readout. In particular, we derive a model for energy selective state readout based on a charge detector response and examine how to optimise the fidelity by choosing correct experimental parameters. Although we focus on single electron spin readout, the theory presented can be applied to other electronic readout techniques in semiconductors that use a reservoir.
Scaling of diffraction intensities near the origin: some rigorous results
Authors: Baake Michael , Grimm Uwe 
Published: May 2019

Abstract
The scaling behaviour of the diffraction intensity near the origin is investigated for (partially) ordered systems, with an emphasis on illustrative, rigorous results. This is an established method to detect and quantify the fluctuation behaviour known under the term hyperuniformity. Here, we consider one-dimensional systems with pure point, singular continuous and absolutely continuous diffraction spectra, which include perfectly ordered cut and project and inflation point sets as well as systems with stochastic disorder.
Measuring grain boundary segregation: tomographic atom probe field ion microscopy (APFIM) vs. analytical scanning transmission electron microscopy (STEM)
Authors: Walther T 
Published: May 2019

Abstract
Grain boundary segregation is an important phenomenon in metallurgy and semiconductor technology. Some recent studies by tomographic atom probe field ion microscopy (APFIM) claim to have measured the interfacial excess of atoms segregated to grain boundaries with ultra-high precision, down to 0.01-0.02 atoms/nm2. This study critically evaluates these claims by simulations. It is shown that atom probe tomography is no ‘magic bullet’ and suffers similar physical constraints as analytical scanning transmission electron microscopy (STEM). Data analyses from both methods have much in common in terms of geometry, performance, systematic and statistical errors. It is shown that an analysis method previously developed for (S)TEM called conceptEM can also successfully be applied to APFIM data.
Propagation of stacking faults from “composite” dislocation cores at low temperature in silicon nanostructures
Authors: Godet Julien , Rabier Jacques 
Published: May 2019

Abstract
The unexpected occurrence of extended stacking faults in silicon nanostructures at high stress and low temperature is discussed. It is shown that those stacking faults result from the operation of “composite” dislocation core structures. It is demonstrated that such cores allow for the propagation of partial dislocations in the shuffle set with the benefit of a low Peierls stress. A classical atomistic calculation confirms indeed that shuffle partial dislocations can move under a shear stress of about 3.3 GPa (5.5% shear strain) at room temperature.
Oxygen precipitate positive charge evolution upon annealing of oxygen implanted silicon
Authors: Danilov D , Vyvenko O , Trushin M , Loshachenko A , Sobolev N 
Published: May 2019

AbstractOxygen precipitates (OPs) formed by the annealing of oxygen implanted silicon samples at diverse temperatures in the ranges from 700°C to 1100°С have been investigated with capacitance-voltage and transmission electron microscopy techniques. An increase of the OP sizes with the increasing temperature was found to accompany with a decrease of the OP embedded positive charge being inversely proportional to the formers. The obtained result showed that the positive charge is localized in SiOxshell of predominantly stoichiometric OP core.
Seaweed extract (Sargassum polycystum) as a preservative on sunscreen cream with the addition of seaweed porridge
Authors: Sipahutar Y H , Albaar N , Purnamasari H B , Kristiany M G , Prabowo D H G 
Published: May 2019

Abstract
The use of sunscreen is highly recommended to protect the health of the skin from ultraviolet radiation. Most commercial sunscreens contain artificial preservatives such as methylparaben, which can be detrimental to health. Preservatives in sunscreen can be substituted with natural ingredients, in which Sargassum polycystum ethanol extract is offered as an alternative in this research. The preservative is made from S. polycystum extract with addition of seaweed porridge of 1:1 S. polycystum and Eecheuma cottoni mixture. The quality of cream is tested using total microbial test, durable power prediction, antioxidant and SPF value. Its physical stability is tested through sensory testing, pH measurement, cyling test and centrifugal test. Butylated hydroxytoluene (BHT) is used as control and is then treated with methylparaben and the extract for comparison, the extract was found to have comparable microbial results with 8 weeks of preservation compared to methylparaben (9 weeks). In addition, the cream shows stability up to one year, a balanced pH according to SNI, shows no foul odor, antioxidant activity (IC50 ) of 105.42, SPF value of 2.00, and moderate to favorable consumer acceptance.
Flavor characteristics and quality of mackerel (Scromberomorus commersonii) “otak-otak” affected by various liquid smoke addition methods
Authors: Susbandi D A , Swastawati F , Suharto S 
Published: May 2019

Abstract
The efforts to utilize fishery resources by processing fish is needed to fulfill food nutrition, such as “otak-otak” made from mackerel (Scromberomorus commersonii). However, “otak-otak” has a relatively short shelf-life, thus preservatives are needed. Liquid smoke of coconut shell is used because it contains phenols which has antibacterial activities. This study aimed to determine the effect of liquid smoke addition to sensory and chemical characteristics on the “otak-otak” and to determine antibacterial activities of liquid smoke using Total Plate Count (TPC). Treatments used were adding 3% liquid smoke (control, mixing, boiling, soaking) and two factors addition of liquid smoke and duration of storage time at ±4°C for 0, 8 and 12 days. The results showed that the variation had a significant effect on hedonic testing. The chemical characteristics of the phenol, moisture, and fat levels had a significant effect on the control method with all treatments, but not in the mixing treatment. Treatment without liquid smoke on the 12th day were unacceptable with a TPC value of 4.97±0.02 log CFU/g, while in mixing, boiling, soaking liquid smoke 3% for 12 days were still acceptable with a value of 4.29±0.1 log CFU/g, 4.55±0.04 log CFU/g, 4.39±0.07 log CFU/g.