bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘Interaction structures’ between depressed adolescents and their therapists in short-term psychoanalytic psychotherapy and cognitive behavioural therapy
Authors: Calderon Ana , Schneider Celeste , Target Mary , Midgley Nick , Goodyer Ian M. , Reynolds Shirley , Barrett Barbara , Byford Sarah , Dubicka Bernadka , Hill Jonathan , Holland Fiona , Kelvin Raphael , Roberts Chris , Senior Rob , Widmer Barry , Wilkinson Paul , Fonagy Peter 
Published: November 2018

Objective: Identify the core ‘interaction structures’ between therapists and depressed adolescents within and across two common forms of psychotherapy. Method: A total of 70 audio-recorded psychotherapy sessions representing short-term psychoanalytic psychotherapy (STPP) and cognitive behavioural therapy (CBT) with youth aged 12–18 years old were coded with the Adolescent Psychotherapy Q-set (APQ), a newly developed instrument. Data included different therapist-patient dyads and stages in treatment and were analysed with cluster analysis. Results: Three distinct interaction structures between therapists and depressed adolescents: two influenced by the therapists’ techniques and one more influenced by the young people’s attitude to therapy. Conclusion: When there is a collaborative working relationship between therapists and depressed young people, the therapy process is influenced by the therapists’ techniques; while when there is a poor working relationship, the techniques used by therapists of different theoretical orientation become more similar with the aim of engaging the young person in the process.
Treatment of terrible triad injuries at a mean follow-up of nine years
Authors: Zaidenberg Ezequiel E , Abrego Mariano O , Donndorff Agustin G , Boretto Jorge G , De Carli Pablo , Gallucci Gerardo L 
Published: November 2018

Background To evaluate the sustainability of the early clinical and radiological outcomes of terrible triad injuries at long-term follow-up. Methods Twelve consecutive patients who underwent fixation of terrible triad injuries with minimum of seven years of follow-up. Functional and radiological outcomes at one year and final follow-up were compared. We assessed Mayo Elbow Performance Score, Quick-Disability of the Arm Shoulder and Hand, and modified-American Shoulder and Elbow Surgeons Scores. Radiological evaluation included Broberg and Morrey classification and Hastings classification for heterotopic ossification. Results The mean age of patients was 55 years, with a median follow-up of 9.3 years. At final evaluation, mean flexion, extension, supination, and pronation were 145°, 6°, 82°, and 80°, respectively; mean Mayo Elbow Performance Score, modified-American Shoulder and Elbow Surgeons Scores, and Quick-Disability of the Arm Shoulder and Hand scores were, respectively, 97, 92, and 4.9 points. There was no statistical difference between early and final follow-up range of motion, Mayo Elbow Performance Score, and Quick-Disability of the Arm Shoulder and Hand. However, radiological changes were observed in 66% of the patients at final follow-up. Two patients underwent reoperation, with final satisfactory results. Conclusion Our results suggested that using a standardized protocol, satisfactory clinical outcomes at early follow-up could be maintained over time. However, early complications and osteoarthritic changes at long-term follow-up can be expected. Level of evidence Therapeutic IV Cases series.
Patient safety situational analysis in Ghana
Authors: Otchi EH , Bannerman C , Lartey S , Amoo KP , Odame E 
Published: November 2018

Objectives The objective of the study was to assess the patient safety situation in Ghana. Specifically, the study aimed at assessing the WHO’s 12 action areas of patient safety. Design This was a descriptive cross-sectional survey. Data collection methods included interviews and observation using WHO adapted questionnaire. Setting The setting was mainly 16 selected hospitals, including two teaching hospitals selected from the northern and southern parts of the Ghana. Participants Respondents were purposively selected based on their position in the health facilities and the type of information required. Main Outcome Measure(s): The main outcome measures were the WHO’s 12 action areas of patient safety. Results The median bed capacity of the hospitals assessed was 93 (min = 60, max = 1812). The country’s highest score (94%) was in the action area of knowledge and learning in patient safety where all (16, 100%) sampled facilities had systems in place for recording adverse events, specific protocols for patient care, etc. Five (5) of the 12 action areas (i.e. national patient policy, hospital acquired infections, surgical safety, patient safety partnerships and patient safety funding) had scores lower than the mean score (66%). Conclusion The key strength identified in the patient safety situational analysis of Ghana was knowledge and learning in patient safety while patient safety surveillance was the weakest action area identified. There were also weaknesses in areas such as national patient policy, healthcare associated infections, surgical safety, patient safety partnerships and patient safety funding, respectively.
Independent yet overlapping pathways ensure the robustness and responsiveness of trans-Golgi network functions in Arabidopsis
Authors: Ravikumar Raksha , Kalbfuß Nils , Gendre Delphine , Steiner Alexander , Altmann Melina , Altmann Stefan , Rybak Katarzyna , Edelmann Holger , Stephan Friederike , Lampe Marko , Facher Eva , Wanner Gerhard , Falter-Braun Pascal , Bhalerao Rishikesh P. , Assaad Farhah F. 
Published: November 2018

ABSTRACT
The trans-Golgi-network (TGN) has essential housekeeping functions in secretion, endocytosis and protein sorting, but also more specialized functions in plant development. How the robustness of basal TGN function is ensured while specialized functions are differentially regulated is poorly understood. Here, we investigate two key regulators of TGN structure and function, ECHIDNA and the Transport Protein Particle II (TRAPPII) tethering complex. An analysis of physical, network and genetic interactions suggests that two network communities are implicated in TGN function and that ECHIDNA and TRAPPII belong to distinct yet overlapping pathways. Whereas ECHIDNA and TRAPPII colocalized at the TGN in interphase cells, their localization diverged in dividing cells. Moreover, ECHIDNA and TRAPPII localization patterns were mutually independent. TGN structure, endocytosis and sorting decisions were differentially impacted in echidna and trappii mutants. Our analyses point to a partitioning of specialized TGN functions, with ECHIDNA being required for cell elongation and TRAPPII for cytokinesis. Two independent pathways able to compensate for each other might contribute to the robustness of TGN housekeeping functions and to the responsiveness and fine tuning of its specialized functions.
Soluble α-synuclein facilitates priming and fusion by releasing Ca2+ from the thapsigargin-sensitive Ca2+ pool in PC12 cells
Authors: Huang Chien-Chang , Chiu Tai-Yu , Lee Tzu-Ying , Hsieh Hsin-Jui , Lin Chung-Chih , Kao Lung-Sen 
Published: November 2018

ABSTRACT
α-Synuclein is associated with Parkinson's disease, and is mainly localized in presynaptic terminals and regulates exocytosis, but its physiological roles remain controversial. Here, we studied the effects of soluble and aggregated α-synuclein on exocytosis, and explored the molecular mechanism by which α-synuclein interacts with regulatory proteins, including Rab3A, Munc13-1 (also known as Unc13a) and Munc18-1 (also known as STXBP1), in order to regulate exocytosis. Through fluorescence recovery after photobleaching experiments, overexpressed α-synuclein in PC12 cells was found to be in a monomeric form, which promotes exocytosis. In contrast, aggregated α-synuclein induced by lactacystin treatment inhibits exocytosis. Our results show that α-synuclein is involved in vesicle priming and fusion. α-Synuclein and phorbol 12-myristate 13-acetate (PMA), which is known to enhance vesicle priming mediated by Rab3A, Munc13-1 and Munc18-1, act on the same population of vesicles, but regulate priming independently. Furthermore, the results show a novel effects of α-synuclein on mobilizing Ca2+ release from thapsigargin-sensitive Ca2+ pools to enhance the ATP-induced [Ca2+]i increase, which enhances vesicle fusion. Our results provide a detailed understanding of the action of α-synuclein during the final steps of exocytosis.
Role of G-proteins and phosphorylation in the distribution of AGS3 to cell puncta
Authors: Vural Ali , Fadillioglu Ersin , Kelesoglu Fatih , Ma Dzwokai , Lanier Stephen M. 
Published: November 2018

ABSTRACT
Activator of G-protein signaling 3 (AGS3, also known as GPSM1) exhibits broad functional diversity and oscillates among different subcellular compartments in a regulated manner. AGS3 consists of a tetratricopeptide repeat (TPR) domain and a G-protein regulatory (GPR) domain. Here, we tested the hypothesis that phosphorylation of the AGS3 GPR domain regulates its subcellular distribution and functionality. In contrast to the cortical and/or diffuse non-homogeneous distribution of wild-type (WT) AGS3, an AGS3 construct lacking all 24 potential phosphorylation sites in the GPR domain localized to cytosolic puncta. This change in localization was revealed to be dependent upon phosphorylation of a single threonine amino acid (T602). The punctate distribution of AGS3-T602A was rescued by co-expression of Gαi and Gαo but not Gαs or Gαq. Following treatment with alkaline phosphatase, both AGS3-T602A and WT AGS3 exhibited a gel shift in SDS-PAGE as compared to untreated WT AGS3, consistent with a loss of protein phosphorylation. The punctate distribution of AGS3-T602A was lost in an AGS3-A602T conversion mutant, but was still present upon T602 mutation to glutamate or aspartate. These results implicate dynamic phosphorylation as a discrete mechanism to regulate the subcellular distribution of AGS3 and associated functionality.
Fibronectin regulates growth factor signaling and cell differentiation in primary lens cells
Authors: VanSlyke Judy K. , Boswell Bruce A. , Musil Linda S. 
Published: November 2018

ABSTRACT
Lens epithelial cells are bound to the lens extracellular matrix capsule, of which laminin is a major component. After cataract surgery, surviving lens epithelial cells are exposed to increased levels of fibronectin, and so we addressed whether fibronectin influences lens cell fate, using DCDML cells as a serum-free primary lens epithelial cell culture system. We found that culturing DCDMLs with plasma-derived fibronectin upregulated canonical TGFβ signaling relative to cells plated on laminin. Fibronectin-exposed cultures also showed increased TGFβ signaling-dependent differentiation into the two cell types responsible for posterior capsule opacification after cataract surgery, namely myofibroblasts and lens fiber cells. Increased TGFβ activity could be identified in the conditioned medium recovered from cells grown on fibronectin. Other experiments showed that plating DCDMLs on fibronectin overcomes the need for BMP in fibroblast growth factor (FGF)-induced lens fiber cell differentiation, a requirement that is restored when endogenous TGFβ signaling is inhibited. These results demonstrate how the TGFβ–fibronectin axis can profoundly affect lens cell fate. This axis represents a novel target for prevention of late-onset posterior capsule opacification, a common but currently intractable complication of cataract surgery.
Cytokine exocytosis and JAK/STAT activation in the Drosophila ovary requires the vesicle trafficking regulator α-Snap
Authors: Saadin Afsoon , Starz-Gaiano Michelle 
Published: November 2018

ABSTRACT
How vesicle trafficking components actively contribute to regulation of paracrine signaling is unclear. We genetically uncovered a requirement for α-soluble NSF attachment protein (α-Snap) in the activation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway during Drosophila egg development. α-Snap, a well-conserved vesicle trafficking regulator, mediates association of N-ethylmaleimide-sensitive factor (NSF) and SNAREs to promote vesicle fusion. Depletion of α-Snap or the SNARE family member Syntaxin1A in epithelia blocks polar cells maintenance and prevents specification of motile border cells. Blocking apoptosis rescues polar cell maintenance in α-Snap-depleted egg chambers, indicating that the lack of border cells in mutants is due to impaired signaling. Genetic experiments implicate α-Snap and NSF in secretion of a STAT-activating cytokine. Live imaging suggests that changes in intracellular Ca2+ are linked to this event. Our data suggest a cell-type specific requirement for particular vesicle trafficking components in regulated exocytosis during development. Given the central role for STAT signaling in immunity, this work may shed light on regulation of cytokine release in humans.
Biphasic recruitment of TRF2 to DNA damage sites promotes non-sister chromatid homologous recombination repair
Authors: Kong Xiangduo , Cruz Gladys Mae Saquilabon , Trinh Sally Loyal , Zhu Xu-Dong , Berns Michael W. , Yokomori Kyoko 
Published: November 2018

ABSTRACT
TRF2 (TERF2) binds to telomeric repeats and is critical for telomere integrity. Evidence suggests that it also localizes to non-telomeric DNA damage sites. However, this recruitment appears to be precarious and functionally controversial. We find that TRF2 recruitment to damage sites occurs by a two-step mechanism: the initial rapid recruitment (phase I), and stable and prolonged association with damage sites (phase II). Phase I is poly(ADP-ribose) polymerase (PARP)-dependent and requires the N-terminal basic domain. The phase II recruitment requires the C-terminal MYB/SANT domain and the iDDR region in the hinge domain, which is mediated by the MRE11 complex and is stimulated by TERT. PARP-dependent recruitment of intrinsically disordered proteins contributes to transient displacement of TRF2 that separates two phases. TRF2 binds to I-PpoI-induced DNA double-strand break sites, which is enhanced by the presence of complex damage and is dependent on PARP and the MRE11 complex. TRF2 depletion affects non-sister chromatid homologous recombination repair, but not homologous recombination between sister chromatids or non-homologous end-joining pathways. Our results demonstrate a unique recruitment mechanism and function of TRF2 at non-telomeric DNA damage sites.
Enigma proteins regulate YAP mechanotransduction
Authors: Elbediwy Ahmed , Vanyai Hannah , Diaz-de-la-Loza Maria-del-Carmen , Frith David , Snijders Ambrosius P. , Thompson Barry J. 
Published: November 2018

ABSTRACT
Human cells can sense mechanical stress acting upon integrin adhesions and respond by sending the YAP (also known as YAP1) and TAZ (also known as WWTR1) transcriptional co-activators to the nucleus to drive TEAD-dependent transcription of target genes. How integrin signaling activates YAP remains unclear. Here, we show that integrin-mediated mechanotransduction requires the Enigma and Enigma-like proteins (PDLIM7 and PDLIM5, respectively; denoted for the family of PDZ and LIM domain-containing proteins). YAP binds to PDLIM5 and PDLIM7 (hereafter PDLIM5/7) via its C-terminal PDZ-binding motif (PBM), which is essential for full nuclear localization and activity of YAP. Accordingly, silencing of PDLIM5/7 expression reduces YAP nuclear localization, tyrosine phosphorylation and transcriptional activity. The PDLIM5/7 proteins are recruited from the cytoplasm to integrin adhesions and F-actin stress fibers in response to force by binding directly to the key stress fiber component α-actinin. Thus, forces acting on integrins recruit Enigma family proteins to trigger YAP activation during mechanotransduction.
This article has an associated First Person interview with the first author of the paper.
An interaction network between the SNARE VAMP7 and Rab GTPases within a ciliary membrane-targeting complex
Authors: Kandachar Vasundhara , Tam Beatrice M. , Moritz Orson L. , Deretic Dusanka 
Published: November 2018

ABSTRACT
The Arf4–rhodopsin complex (mediated by the VxPx motif in rhodopsin) initiates expansion of vertebrate rod photoreceptor cilia-derived light-sensing organelles through stepwise assembly of a conserved trafficking network. Here, we examine its role in the sorting of VAMP7 (also known as TI-VAMP) – an R-SNARE possessing a regulatory longin domain (LD) – into rhodopsin transport carriers (RTCs). During RTC formation and trafficking, VAMP7 colocalizes with the ciliary cargo rhodopsin and interacts with the Rab11–Rabin8–Rab8 trafficking module. Rab11 and Rab8 bind the VAMP7 LD, whereas Rabin8 (also known as RAB3IP) interacts with the SNARE domain. The Arf/Rab11 effector FIP3 (also known as RAB11FIP3) regulates VAMP7 access to Rab11. At the ciliary base, VAMP7 forms a complex with the cognate SNAREs syntaxin 3 and SNAP-25. When expressed in transgenic animals, a GFP–VAMP7ΔLD fusion protein and a Y45E phosphomimetic mutant colocalize with endogenous VAMP7. The GFP–VAMP7-R150E mutant displays considerable localization defects that imply an important role of the R-SNARE motif in intracellular trafficking, rather than cognate SNARE pairing. Our study defines the link between VAMP7 and the ciliary targeting nexus that is conserved across diverse cell types, and contributes to general understanding of how functional Arf and Rab networks assemble SNAREs in membrane trafficking.
Alpha-synuclein fibrils recruit TBK1 and OPTN to lysosomal damage sites and induce autophagy in microglial cells
Authors: Bussi Claudio , Peralta Ramos Javier M. , Arroyo Daniela S. , Gallea Jose I. , Ronchi Paolo , Kolovou Androniki , Wang Ji M. , Florey Oliver , Celej Maria S. , Schwab Yannick , Ktistakis Nicholas T. , Iribarren Pablo 
Published: November 2018

ABSTRACT
Autophagic dysfunction and protein aggregation have been linked to several neurodegenerative disorders, but the exact mechanisms and causal connections are not clear and most previous work was done in neurons and not in microglial cells. Here, we report that exogenous fibrillary, but not monomeric, alpha-synuclein (AS, also known as SNCA) induces autophagy in microglial cells. We extensively studied the dynamics of this response using both live-cell imaging and correlative light-electron microscopy (CLEM), and found that it correlates with lysosomal damage and is characterised by the recruitment of the selective autophagy-associated proteins TANK-binding kinase 1 (TBK1) and optineurin (OPTN) to ubiquitylated lysosomes. In addition, we observed that LC3 (MAP1LC3B) recruitment to damaged lysosomes was dependent on TBK1 activity. In these fibrillar AS-treated cells, autophagy inhibition impairs mitochondrial function and leads to microglial cell death. Our results suggest that microglial autophagy is induced in response to lysosomal damage caused by persistent accumulation of AS fibrils. Importantly, triggering of the autophagic response appears to be an attempt at lysosomal quality control and not for engulfment of fibrillar AS.
This article has an associated First Person interview with the first author of the paper.
DIGITAL MARKETING PERFORMANCE EVALUATION METHODS
Authors: Gaitniece Elina 
Published: November 2018

The share of digital marketing from total marketing spending is increasing fast year by year. Still the majority of companies admit that they are missing clear methods on how to evaluate (positive/negative) effects obtained using digital marketing. As this field of digital marketing is relatively new, there are no firm and widely recognized measurement methods resulting in marketing specialists being confused by an overwhelming amount and variety of different digital marketing metrics and large amounts of data. Therefore, the aim of this paper is to list the existing methods which could help marketers to evaluate their digital marketing campaign effectiveness and to discuss these methods’ effectiveness with experts. To achieve this aim, the author defined the following objectives for the paper: 1) to explore which digital marketing campaign effectiveness evaluation methods can be found in realized scientific research so far; 2) to assess the strengths and weaknesses of each method by conducting expert interviews; 3) to determine the practical application opportunities for each identified method; and 4) to draw conclusions and make recommendations for the practical usage of the studied methods. Research methods used were secondary data analysis and expert interviews. The secondary data analysis contained reviews of scientific research results in the field mentioned above. The expert interviews covered in-depth interviews with the main stakeholders – leading online retailers in the Baltics and digital marketing experts. The results show that the conversion evaluation method is most frequently used in theory and in practice as it can be best connected with direct business goals to increase sales. It also highlights that each method has some benefits to be used in specific situations, with specific aims.
ANALYSIS OF BITCOIN MARKET EFFICIENCY BY USING MACHINE LEARNING
Authors: Hirano Yuki , Pichl Lukáš , Eom Cheoljun , Kaizoji Taisei 
Published: November 2018

The issue of market efficiency for cryptocurrency exchanges has been largely unexplored. Here we put Bitcoin, the leading cryptocurrency, on a test by studying the applicability of the Efficient Market Hypothesis by Fama from two viewpoints: (1) the existence of profitable arbitrage spread among Bitcoin exchanges, and (2) the possibility to predict Bitcoin prices in EUR (time period 2013-2017) and the direction of price movement (up or down) on the daily trading scale. Our results show that the Bitcoin market in the time period studied is partially inefficient. Thus the market process is predictable to a degree, hence not a pure martingale. In particular, the F-measure for XBTEUR time series obtained by three major recurrent neural network based machine learning methods was about 67%, i.e. a way above the unbiased coin tossing odds of 50% equal chance.
ANALYSIS OF WORK EFFICIENCY IN HARD COAL MINING IN POLAND
Authors: Rybak Aurelia , Włodarczyk Ewelina 
Published: November 2018

Motivation: This article presents the analysis of work efficiency in hard coal mining in Poland. Labour costs in Polish mining enterprises account for over 40% of the total production cost. For this reason, the labour productivity of employees has a key impact on the final operating profit.Problem statement: In the case of Polish coal companies, the efficiency is the index value of which particular restructuration programs have attempted to increase for years. However, because of the effect of overstaffing and a decrease in hard coal exploitation, the task was impossible.Approach and results: In this article the effects of the latest recovery programme have been presented, the production efficiency index has been determined; the rate of changes in production volume has been presented as well as the employment figure and the average salary in the Polish mining industry in the recent decade. Moreover, the prognosis of the employment figure using the ARIMA and ARMAX class model was conducted.Conclusions: It should be noted that in the last four years a significant reduction in the employment figure has been onserved in the hard coal mining industry. This figure has been adjusted to the production volume level. This, in turn, has positively influenced the work efficiency coefficient level.