bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systemic Injection of Substance P Promotes Murine Calvarial Repair Through Mobilizing Endogenous Mesenchymal Stem Cells
Authors: Zhang Yueling , An Shu , Hao Jin , Tian Feng , Fang Xinyi , Wang Jun 
Published: August 2018

AbstractCraniofacial defect is a critical problem in dental clinic, which has a tremendous impact on patients’ quality of life. Mesenchymal stem cell-based therapy has emerged as a promising approach for tissue defect repair. However, reduced survival after mesenchymal stem cells (MSCs) transplantation remains as a major problem in this area, which hampers the outcome of regeneration. Recently, the mechanism to mobilize endogenous MSCs for tissue regeneration has received increasing attentions, as it does not require exogenous cell transplantation. The primary goal of this study was to confirm the role of intravenous substance P in mobilizing endogenous CD45−CD11b−CD29+ MSCs in critical-sized bone defect animals and to investigate the effects of substance P on calvarial bone repair. Flow cytometry analyses revealed that intravenous substance P promoted the mobilization of endogenous CD45−CD11b−CD29+ MSCs after bone defect. In addition, Micro-CT showed that intravenous substance P improved the outcomes of calvarial bone repair. Furthermore, we discovered that systemic injection of substance P attenuated inflammation and enhanced the survival of the local-transplanted GFP+ MSCs. Our findings suggested that substance P together with its mobilized CD45−CD11b−CD29+ MSCs helped improve calvarial defect repair through regulating inflammatory conditions and promoting the survival of local-transplanted cells.
A Serine/Threonine Kinase 16-Based Phospho-Proteomics Screen Identifies WD Repeat Protein-1 As A Regulator Of Constitutive Secretion
Authors: López-Coral Alfonso , Striz Anneliese C. , Tuma Pamela L. 
Published: August 2018

AbstractThe plasma membrane of polarized hepatocytes is functionally divided into two domains: the apical and basolateral. Our focus is to define the molecular basis of polarized protein sorting of newly-synthesized membrane and secretory proteins in WIF-B cells, an excellent model system for polarized hepatocytes. We determined that MAL2 (myelin and lymphocyte protein 2) and its binding partner, serine/threonine kinase 16 (STK16) regulate basolateral constitutive secretion. Because STK16 is a constitutively active kinase, we reasoned that constitutively phosphorylated substrates must participate in constitutive secretion. To identify either STK16 substrates or other proteins that regulate constitutive secretion, we took a proteomics approach. Post-nuclear supernatants from cells expressing wild type or a kinase-dead (E202A) STK16 were separated on 2D gels and immunoblotted with antibodies against phospho-serine/threonine residues. Sixteen spots were identified from E202A-expressing cells that reproducibly displayed decreased immunoreactivity. From these spots, 28 proteins were identified as possible STK16 substrates. Out of these 28 possible substrates, 25% of them encode predicted STK16 phosphorylation consensus sites, with WD repeat containing protein-1 (WDR1) encoding two such sites. Based on this finding and on the finding that actin remodeling is required for hepatic secretion, we further confirmed that WDR1 is a phosphoprotein that regulates secretion.
A steam-based method to investigate biofilm
Authors: Tasse Jason , Cara Andréa , Saglio Maude , Villet Régis , Laurent Frédéric 
Published: August 2018

AbstractBiofilm has become a major topic of interest in medical, food, industrial, and environmental bacteriology. To be relevant, investigation of biofilm behavior requires effective and reliable techniques. We present herein a simple and robust method, adapted from the microplate technique, in which steam is used as a soft washing method to preserve biofilm integrity and to improve reproducibility of biofilm quantification. The kinetics of steam washing indicated that the method is adapted to remove both planktonic bacteria and excess crystal violet (CV) staining for S. aureus, S. epidermidis, S. carnosus, P. aeruginosa, and E. coli biofilm. Confocal laser scanning microscopy confirmed that steam washing preserved the integrity of the biofilm better than pipette-based washing. We also investigated the measurement of the turbidity of biofilm resuspended in phosphate-buffered saline (PBS) as an alternative to staining with CV. This approach allows the discrimination of biofilm producer strains from non-biofilm producer strains in a way similar to CV staining, and subsequently permits quantification of viable bacteria present in biofilm by culture enumeration from the same well. Biofilm quantification using steam washing and PBS turbidity reduced the technical time needed, and data were highly reproducible.
An adjustment in BMP4 function represents a treatment for diabetic nephropathy and podocyte injury
Authors: Fujita Yui , Tominaga Tatsuya , Abe Hideharu , Kangawa Yumi , Fukushima Naoshi , Ueda Otoya , Jishage Kou-ichi , Kishi Seiji , Murakami Taichi , Saga Yumiko , Kanwar Yashpal S. , Nagai Kojiro , Doi Toshio 
Published: August 2018

AbstractPodocyte injury has been proposed to play an important role in diabetic nephropathy; however, its pathological mechanism remains unclear. We have shown that bone morphogenetic protein 4 (BMP4) signaling leads to the glomerular changes characteristic of this disorder. To analyze the molecular mechanism of podocyte injury, the effect of BMP4 was investigated using streptozotocin (STZ)-induced, Bmp4 heterozygous knockout (Bmp4+/−) and podocyte-specific Bmp4 knockout mice. Mice with STZ-induced diabetes exhibited glomerular matrix hyperplasia and decreased numbers of podocyte nucleus-specific WT1-positive cells. The number of podocytes and proteinuria were improved in both diabetic Bmp4 knockout mouse models compared to the effects observed in the control mice. The effect of BMP4 overexpression on Bmp4-induced or podocyte-specific transgenic mice was examined. Tamoxifen-induced Bmp4-overexpressing mice exhibited mesangial matrix expansion and decreased numbers of WT1-positive cells. Podocyte-specific Bmp4-overexpressing mice displayed increased kidney BMP4 expression and mesangial matrix expansion but decreased nephrin expression and numbers of WT1-positive cells. Both lines of Bmp4-overexpressing mice exhibited increased albuminuria. In cultured podocytes, BMP4 increased phospho-p38 levels. BMP4 decreased nephrin expression but increased cleaved caspase-3 levels. p38 suppression inhibited caspase-3 activation. Apoptosis was confirmed in STZ-diabetic glomeruli and Bmp4-overexpressing mice. Bmp4 +/− mice with diabetes displayed reduced apoptosis. Based on these data, the BMP4 signaling pathway plays important roles in the development of both podocyte injury and mesangial matrix expansion in diabetic nephropathy.
Phytolith analysis for differentiating between broomcorn millet (Panicum miliaceum) and its weed/feral type (Panicum ruderale)
Authors: Zhang Jianping , Lu Houyuan , Liu Minxuan , Diao Xianmin , Shao Konglan , Wu Naiqin 
Published: August 2018

AbstractDomestication of broomcorn millet (Panicum miliaceum) is one of the most significant events in prehistoric East Asia, providing sufficient food supply for the explosive growth of Neolithic populations and the transition into complex societies. However, to date, the process of broomcorn millet domestication is still largely unknown, partly due to the lack of clear diagnostic tools for distinguishing between millet and its related wild grasses in archaeological samples. Here, we examined the percentage of silicified epidermal long-cell undulated patterns in the glume and palea from inflorescence bracts in 21 modern varieties of broomcorn millet and 12 weed/feral-type Panicum ruderale collected across northern China. Our results show that the percentage of ηIII patterns in domesticated broomcorn millet (23.0% ± 5.9%; n = 63) is about 10% higher than in P. ruderale (10.8% ± 5.8%; n = 36), with quartiles of 17.2–28.3% and 5.1–15.5%, respectively. Owing to the increase in ηIII pattern percentage correlates significantly with a decrease in the grain length/width ratio, in the absence of exact wild ancestors of broomcorn millet, the characterization of phytolith differences between P. ruderale and P. miliaceum thus becomes an alternative approach to provide insight into origin of broomcorn millet.
Experimentally validated full-vectorial model of wavelength multicasting via four-wave mixing in straight waveguides
Authors: Guo Kai , Feng Jiacheng , Shi Xiaodong , Li Jiehui , Gao Minghong , Jing Hui , Wang Xiaolin , Yang Junbo , Ou Haiyan 
Published: August 2018

AbstractWe derive full-vectorial nonlinear propagation equations of dual-pumped four-wave mixing in straight waveguides, which are valid in characterizing the one-to-six wavelength multicasting. Special attention is paid to the resulting idler wavelengths and their conversion efficiency, which enables the optimization of the experimental designs, including the incident wavelength and the power of pumps and signal. We validate the model by comparing the numerical simulation to the experimental measurement in a silicon-on-insulator waveguide, for the first time to our best knowledge, and achieve a good agreement. We further derive the general form of the proposed model for the case of using multiple,pumps, which holds a potential to numerically predict the performance of complex wavelength multicasting, and essentially guide the waveguide designs.
The surface reactivity of iron oxide nanoparticles as a potential hazard for aquatic environments: A study on Daphnia magna adults and embryos
Authors: Magro Massimiliano , De Liguoro Marco , Franzago Eleonora , Baratella Davide , Vianello Fabio 
Published: August 2018

AbstractNano-ecotoxicology is extensively debated and nanomaterial surface reactivity is an emerging topic. Iron oxide nanoparticles are widely applied, with organic or inorganic coatings for stabilizing their suspensions. Surface active maghemite nanoparticles (SAMNs) are the unique example of naked iron oxide displaying high colloidal and structural stability in water and chemical reactivity. The colloidal behavior of SAMNs was studied as a function of the medium salinity and protocols of acute and chronic toxicity on Daphnia magna were consequently adapted. SAMN distribution into the crustacean, intake/depletion rates and swimming performances were evaluated. No sign of toxicity was detected in two model organisms from the first trophic level (P. subcapitata and L. minor). In D. magna, acute EC50 values of SAMN was assessed, while no sub-lethal effects were observed and the accumulation of SAMNs in the gut appeared as the sole cause of mortality. Fast depuration and absence of delayed effects indicated no retention of SAMNs within the organism. In spite of negligible toxicity on D. magna adults, SAMN surface reactivity was responsible of membrane bursting and lethality on embryos. The present study offers a contribution to the nascent knowledge concerning the impact of nanoparticle surface reactivity on biological interfaces.
Whole grain intake associated molecule 5-aminovaleric acid betaine decreases β-oxidation of fatty acids in mouse cardiomyocytes
Authors: Kärkkäinen Olli , Tuomainen Tomi , Koistinen Ville , Tuomainen Marjo , Leppänen Jukka , Laitinen Tuomo , Lehtonen Marko , Rysä Jaana , Auriola Seppo , Poso Antti , Tavi Pasi , Hanhineva Kati 
Published: August 2018

AbstractDespite epidemiological evidence showing that diets rich in whole grains reduce the risk of chronic life-style related diseases, biological mechanisms for these positive effects are mostly unknown. Increased 5-aminovaleric acid betaine (5-AVAB) levels in plasma and metabolically active tissues such as heart have been associated with consumption of diets rich in whole grains. However, biological effects of 5-AVAB are poorly understood. We evaluated 5-AVAB concentrations in human and mouse heart tissue (3–22 µM and 38–78 µM, respectively) using mass spectrometry. We show that 5-AVAB, at physiological concentration range, dose-dependently inhibits oxygen consumption due to β-oxidation of fatty acids, but does not otherwise compromise mitochondrial respiration, as measured with oxygen consumption rate in cultured mouse primary cardiomyocytes. We also demonstrate that this effect is caused by 5-AVAB induced reduction of cellular L-carnitine. Reduced L-carnitine levels are at least partly mediated by the inhibition of cell membrane carnitine transporter (OCTN2) as evaluated by in silico docking, and by siRNA mediated silencing of OCTN2 in cultured cardiomyocytes. 5-AVAB caused inhibition of β-oxidation of fatty acids is a novel mechanism on how diets rich in whole grains may regulate energy metabolism in the body. Elucidating potentially beneficial effects of 5-AVAB e.g. on cardiac physiology will require further in vivo investigations.
Low-Salt or Salt-Free Dyeing of Cotton Fibers with Reactive Dyes using Liposomes as Dyeing/Level-Dyeing Promotors
Authors: Ru Jidong , Qian Xueren , Wang Ying 
Published: August 2018

AbstractThe main aim of this investigation was to promote the dyeing and level-dyeing effect of reactive dyes on cotton-fiber dyeing by encapsulating reactive dyes in liposomes as an alternative to sodium chloride. The results obtained indicated that liposomes, especially cationic liposomes, have a remarkable level-dyeing promoting effect on cotton fibers, although the dyeing promoting effect was not as good as that of sodium chloride. The optimum dyeing and level-dyeing effects were achieved at a dye-fixing temperature of 85 °C, sodium carbonate concentration of 10 g/L and dye dosage of 2% (on the basis of oven-dry cotton fibers) when liposomes were used as the dyeing and level-dyeing promoters. The combination of cationic liposomes and sodium chloride can significantly promote both the dyeing and level-dyeing of cotton fibers. These results indicated the potential of cationic liposomes as novel dyeing and level-dyeing promoters or microencapsulated dye wall materials for reactive-dye dyeing applications.
Prolonged co-treatment with HGF sustains epithelial integrity and improves pharmacological rescue of Phe508del-CFTR
Authors: Matos Ana M. , Gomes-Duarte Andreia , Faria Márcia , Barros Patrícia , Jordan Peter , Amaral Margarida D. , Matos Paulo 
Published: August 2018

AbstractCystic fibrosis (CF), the most common inherited disease in Caucasians, is caused by mutations in the CFTR chloride channel, the most frequent of which is Phe508del. Phe508del causes not only intracellular retention and premature degradation of the mutant CFTR protein, but also defective channel gating and decreased half-life when experimentally rescued to the plasma membrane (PM). Despite recent successes in the functional rescue of several CFTR mutations with small-molecule drugs, the folding-corrector/gating-potentiator drug combinations approved for Phe508del-CFTR homozygous patients have shown only modest benefit. Several factors have been shown to contribute to this outcome, including an unexpected intensification of corrector-rescued Phe508del-CFTR PM instability after persistent co-treatment with potentiator drugs. We have previously shown that acute co-treatment with hepatocyte growth factor (HGF) can significantly enhance the chemical correction of Phe508del-CFTR. HGF coaxes the anchoring of rescued channels to the actin cytoskeleton via induction of RAC1 GTPase signalling. Here, we demonstrate that a prolonged, 15-day HGF treatment also significantly improves the functional rescue of Phe508del-CFTR by the VX-809 corrector/VX-770 potentiator combination, in polarized bronchial epithelial monolayers. Importantly, we found that HGF treatment also prevented VX-770-mediated destabilization of rescued Phe508del-CFTR and enabled further potentiation of the rescued channels. Most strikingly, prolonged HGF treatment prevented previously unrecognized epithelial dedifferentiation effects of sustained exposure to VX-809. This was observed in epithelium-like monolayers from both lung and intestinal origin, representing the two systems most affected by adverse symptoms in patients treated with VX-809 or the VX-809/VX-770 combination. Taken together, our findings strongly suggest that co-administration of HGF with corrector/potentiator drugs could be beneficial for CF patients.
Crystallographic evidence for unexpected selective tyrosine hydroxylations in an aerated achiral Ru–papain conjugate
Authors: Cherrier Mickaël V , Amara Patricia , Talbi Barisa , Salmain Michèle , Fontecilla-Camps Juan C 
Published: August 2018

Abstract
The X-ray structure of an aerated achiral Ru–papain conjugate has revealed the hydroxylation of two tyrosine residues found near the ruthenium ion. The most likely mechanism involves a ruthenium-bound superoxide as the reactive species responsible for the first hydroxylation and the resulting high valent Ru(iv)˭O species for the second one.
The spectacular cluster chain Abell 781 as observed with LOFAR, GMRT, and XMM-Newton
Authors: Botteon A. , Shimwell T. W. , Bonafede A. , Dallacasa D. , Gastaldello F. , Eckert D. , Brunetti G. , Venturi T. , van Weeren R. J. , Mandal S. , Brüggen M. , Cassano R. , de Gasperin F. , Drabent A. , Dumba C. , Intema H. T. , Hoang D. N. , Rafferty D. , Röttgering H. J. A. , Savini F. , Shulevski A. , Stroe A. , Wilber A. 
Published: August 2018

Context. A number of merging galaxy clusters show the presence of large-scale radio emission associated with the intra-cluster medium (ICM). These synchrotron sources are generally classified as radio haloes and radio relics.
Aims. Whilst it is commonly accepted that mergers play a crucial role in the formation of radio haloes and relics, not all the merging clusters show the presence of giant diffuse radio sources and this provides important information concerning current models. The Abell 781 complex is a spectacular system composed of an apparent chain of clusters on the sky. Its main component is undergoing a merger and hosts peripheral emission that is classified as a candidate radio relic and a disputed radio halo.
Methods. We used new LOw Frequency ARay (LOFAR) observations at 143 MHz and archival Giant Metrewave Radio Telescope (GMRT) observations at 325 and 610 MHz to study radio emission from non-thermal components in the ICM of Abell 781. Complementary information came from XMM-Newton data, which allowed us to investigate the connection with the thermal emission and its complex morphology.
Results. The origin of the peripheral emission is still uncertain. We speculate that it is related to the interaction between a head tail radio galaxy and shock. However, the current data allow us only to set an upper limit of ℳ <  1.4 on the Mach number of this putative shock. Instead, we successfully characterise the surface brightness and temperature jumps of a shock and two cold fronts in the main cluster component of Abell 781. Their positions suggest that the merger is involving three substructures. We do not find any evidence for a radio halo either at the centre of this system or in the other clusters of the chain. We place an upper limit to the diffuse radio emission in the main cluster of Abell 781 that is a factor of 2 below the current radio power-mass relation for giant radio haloes.
Management und Abrechnung der BK 5103 − Tipps und Tricks
Authors: Wehrmann W. , John S. , Skudlik C. 
Published: August 2018

ZusammenfassungAufgrund des sich aus der Aufnahme der Berufskrankheit Nr. 5103 „Plattenepithelkarzinome oder multiple aktinische Keratosen der Haut durch natürliche UV-Strahlung“ in die Berufskrankheitenliste ergebenden Bedarfs der Versorgung von Patienten mit beruflich verursachtem Hautkrebs in der hautärztlichen Praxis, wurden seitens der ständigen Gebührenkommission neue Gebührennummern für die UV-GOÄ beschlossen. Diese neuen Gebührennummern bieten nunmehr den behandelnden Dermatologen von Patienten mit berufsbedingtem Hautkrebs einschließlich von aktinischen Keratosen bzw. Feldkanzerisierung Sicherheit in der Abrechnung und eine leistungsbezogene Vergütung.
Comparative Studies of Blood Coagulation in Hibernating and Non-Hibernating Frogs (Rana tigrina)
Authors: Ahmad Nasreen , Dube B , Agarwal G P , Dube Rama Kanta 
Published: August 2018

Summary1. During hibernation in frogs (Rana tigrina) there occurs prolongation of several clotting tests, viz, whole blood clotting time, plasma recalcification time, cephalin time and prothrombin time.2. The ambient body temperature of the frog during winter hibernation is low and the retarded blood clotting at lower temperature may play an important protective role against intravascular thrombosis.3. Shorter plasma recalcification time in low-spun plasma as compared to high-spun plasma indicated the presence of procoagulant activity in platelets/leucocytes of frogs.
Kinetic Studies on the Selectivity of a Synthetic Thrombin-Inhibitor Using Synthetic Peptide Substrates
Authors: Huikata A , Okamoto S , Kikumoto R , Tamao Y 
Published: August 2018

SummaryThe synthetic thrombin-inhibitor termed No. 205 (N-α-dansyl-L-arginine-4-ethyl-piperidine amide) found in our laboratories was studied kinetically using synthetic peptide substrates. The following results were obtained. 1. No. 205 inhibited thrombin competitively with bz-Phe-Val-Arg-pNA and the Ki value obtained was extremely small, 3.7 × 10-8 M. 2. No. 205 also inhibited trypsin competitively with bz-Phe-Val-Arg-pNA but the Ki value obtained was far larger than that for thrombin, 1.0 × 10-5 M. 3. No. 205 inhibited F. Xa, plasmin and urokinase only to a small extent when estimated using 2 × 10-4 M D-Val-Leu- Lys-pNA, bz-Ile-Glu-Gly-Arg-pNA and Glu-Gly-Arg-pNA, respectively. 4. No. 205 differed from APPA in its specific inhibitory spectrum for thrombin as compared to trypsin, plasmin and F. Xa. The above results indicate that No. 205 is an extremely potent and highly selective reversible thrombin-inhibitor.
Biological Properties of the Thromboplastins and Plasmas Included in the ICTH/ICSH Collaborative Study on Prothrombin Time Standardization
Authors: Loeliger E A , van Halem-Visser L P 
Published: August 2018

SummaryThe fourteen types of thromboplastin included in the ICTH/ICSH Collaborative Study on Prothrombin Time Standardization were investigated with respect to their sensitivity for factor VII, activation products, and PIVKAs. All of these thromboplastins displayed sufficient factor VII sensitivity, and all were sensitive to activation products. After correction for the overall sensitivity slope, rabbit plain thromboplastins were the most sensitive preparations and human as well as rabbit diluted the least sensitive; bovine brain thromboplastin lost its exceptional position. Sensitivity to activation products explains why factors II, VII, and X present in artificially prepared abnormal plasmas may be difficult to assess accurately. Also, all thromboplastins appear to be sensitive for both PIVKA VII and PIVKA X. PIVKA VII acts as a procoagulant which increases the amount of factor VII measured by the one-stage assay technique. PIVKA X inhibits and thus reduces the activity of factor X. The procoagulant activity of PIVKA VII is particulary pronounced for the two lung-brain rabbit thromboplastins (Lyoplastin and Simplastin), whereas the anticoagulant action of PIVKA X is strongest with bovine brain thromboplastin (Thrombotest). Considered as a group, rabbit thromboplastins appear to be less sensitive for PIVKA X and more sensitive for PIVKA VII.As judged from thromboplastin calibration results, all three types of lyophilized reference plasmas are to some degree distinguishable from fresh patient plasmas. Primary calibration of a thromboplastin must therefore still be performed with freshly prepared normal as well as patient plasmas.