bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the genetic relationship ofTrichosporon asahiiisolates using a newly developed microsatellite typing assay
Authors: Francisco Elaine C. , Fernández Norma B. , Carbia Mauricio , Dieleman Chendo , Bostanaru-Iliescu Andra-Cristina , Houbraken Jos , Colombo Arnaldo L. , Hagen Ferry 
Published: November 2024

AbstractThe basidiomycetous yeast-like fungusTrichosporon asahiiis increasingly encountered in the clinic and has been known to cause nosocomial infections. Here, we aim to develop a microsatellite typing tool to investigate the genetic relatedness ofTrichosporon asahiiisolates. Six microsatellite markers were selected from a nanopore long-read sequencing de novo assembled genome of theT. asahiitype-strain CBS 2479. PCR reactions amplified three di-, tri-, or tetranucleotide repeats, respectively. All six microsatellite markers were used to analyze 111T. asahiiisolates, including single and related-patient isolates from clinical and environmental sources. Each marker exhibited between 11 and 37 alleles in this population, resulting in 71 different microsatellite genotypes. The Simpson’s diversity index ranged from 0.6452 to 0.8280 for the individual markers, and for the combined set of microsatellites 0.9793, indicating the high discriminatory power of the reported panel. In summary, this panel combines high reproducibility and specificity, making it suitable for use in epidemiological studies ofT. asahiiand in investigations of potential outbreaks caused by this pathogen.
A conservedin vivoburn wound infection model for diverse pathogenic fungi
Authors: Mercado Soto Nayanna M. , Horn Adam , Keller Nancy P. , Huttenlocher Anna , Wagner Andrew S. 
Published: November 2024

AbstractSecondary fungal infections represent a major complication following thermal injuries. However, the mechanisms of fungal colonization of burn tissue and how the host subsequently responds to fungi within this niche remain unclear. We have previously reported a zebrafish model of thermal injury that recapitulates many of the features of human burn wounds. Here, we characterize host-fungal interaction dynamics within the burn wound niche using two of the most common fungal pathogens isolated from burn injuries,Aspergillus fumigatusandCandida albicans. BothA. fumigatusandC. albicanscolonize burned tissue in zebrafish larvae and induce a largely conserved innate immune response following colonization. Using drug-induced cell depletion strategies and transgenic zebrafish lines with impaired innate immune function, we found that macrophages control fungal burden while neutrophils primarily control invasive hyphal growth at the early stages of infection. However, we also found that loss of either immune cell can be compensated by the other at the later stages of infection, and that fish with both macrophage and neutrophil deficiencies show more invasive hyphal growth that is sustained throughout the infection process, suggesting redundancy in their antifungal activities. Finally, we demonstrate thatC. albicansstrains with increased β(1,3)-glucan exposure are cleared faster from the burn wound, demonstrating a need for shielding this immunogenic cell wall epitope for successful fungal colonization of burn tissue. Together, our findings support the use of zebrafish larvae as a model to study host-fungal interaction dynamics within burn wounds.ImportanceSecondary fungal infections within burn wound injuries are a significant problem that delay wound healing and increase the risk of patient mortality. Currently, little is known about how fungi colonize and infect burn tissue or how the host responds to pathogen presence. In this report, we expand upon an existing thermal injury model using zebrafish larvae to begin to elucidate both the host immune response to fungal burn colonization and fungal mechanisms for persistence within burn tissue. We found that bothAspergillus fumigatusandCandida albicans, common fungal burn wound isolates, successfully colonize burn tissue and are effectively cleared in immunocompetent zebrafish by both macrophages and neutrophils. We also find thatC. albicansmutants harboring mutations that impact their ability to evade host immune system recognition are cleared more readily from burn tissue. Collectively, our work highlights the efficacy of using zebrafish to study host-fungal interaction dynamics within burn wounds.
Loss of TMEM55B modulates lipid metabolism through dysregulated lipophagy and mitochondrial function
Authors: Qin Yuanyuan , Teker Sheila , Cunza Nilsa La , Theusch Elizabeth , Yang Neil , Venkatesan Leela , Su Julia , Krauss Ronald M. , Lakkaraju Aparna , Mattis Aras N. , Medina Marisa W. 
Published: November 2024

AbstractLipophagy is a form of selective autophagy that targets the lipid droplets for lysosomal decay and has been implicated in the onset and progression of metabolic dysfunction-associated steatotic liver disease (MASLD). Factors that augment lipophagy have been identified as targets for MASLD therapeutic development. TMEM55B is a key regulator of lysosomal positioning which is critical for the lysosome fusion with the autophagosome, but less studied. Here, we demonstrate that inhibition of TMEM55B in murine models accelerates MASLD onset and progression. In cellular models, TMEM55B deficiency enhances lipophagy, leading to increased fatty acid release from lysosomes to mitochondria but simultaneously impairs mitophagy, causing an accumulation of dysfunctional mitochondria. This imbalance leads to increased lipid accumulation and oxidative stress, worsening MASLD. These findings underscore the importance of lysosomal positioning in lipid metabolism and suggest that augmenting lipophagy may exacerbate disease in the context of mitochondrial dysfunction.
Diagnostic-avoidingChlamydia trachomatisvariants detected in cervical and endometrial specimens from women during 16S microbiome profiling
Authors: Jeong Sangmi , Tollison Tammy , Brochu Hayden , Chou Hsuan , Yu Tammy , Baghaie Priyanka , Yount Kacy S. , Darville Toni , Wiesenfeld Harold C , Hillier Sharon L , Peng Xinxia , O’Connell Catherine M. 
Published: November 2024

AbstractBackgroundPerformance of a 16S rRNA analysis of the cervicovaginal microbiome of 220 participants recruited into the T Cell Response against Chlamydia (TRAC) cohort between February 2011 and August 2014 in Allegheny County, Pennsylvania USA detected DNA encoding chlamydial 16S rRNA in samples from seven participants whose tests were negative forChlamydia trachomatis(CT) and DNA encoding gonococcal 16S rRNA from five participants whose tests were negative forNeisseria gonorrhoeae(NG) infection with the Aptima Combo2 assay (Hologic).MethodsWe used targeted PCR amplification followed by sequencing to characterize the chlamydial 23S rRNA locus and qPCR to detect gonococcal DNA in residual diagnostic swab eluates or DNA used to generate 16S rRNA libraries.ResultsDiscrepant specimens that contained chlamydial DNA carried a diagnostic-avoidant, G1526A variant in the 23S rRNA locus identical to variants previously detected in Finland, Denmark, and the UK. PCR validation of gonococcal DNA was confirmed for all participants whose tests were negative, with stochastic effects consistent with infection levels close to the limit of detection by the diagnostic assay.ConclusionsThese data indicate that this probe-avoidant CT mutant, and possibly others, were circulating in the northeastern US prior to their detection and characterization in 2019. Although infrequent, documentation of false negative results for CT indicates a need for clinicians to consider performance of a second test that uses alternate PCR targets if patients have persistent symptoms or have known contact to an infected sex partner and their initial NAAT is negative.
A necroptotic-to-apoptotic signaling axis underlies inflammatory bowel disease
Authors: Pang Jiyi , Al-Ani Aysha H. , Patel Komal M. , Young Samuel N. , Kong Isabella , Chen Jin-jin , Barrios Marilou , Rickard James A. , Chen Siqi , Foroughi Siavash , Cawthorne Wayne , Jacobsen Annette V. , Jois Asha , Weir Ashley L. , Whitehead Lachlan W. , Rajasekhar Pradeep , Horne Christopher R. , Azeez Imadh , Tan Tao , Liang Weiwei , Sivanesan Suresh , Metz Andrew , Patwardhan Ash , Shea Natalie , Iyngkaran Guru , Schneider Daniel , Elford Alexander T. , Beattie William , Macrae Finlay , Liccardi Gianmaria , Walczak Henning , Zhang Yuxia , Sieber Oliver M. , Spelman Tim , Giulino-Roth Lisa , Hawkins Edwin D. , Rogers Kelly L. , Bowden Rory , Nicholson Sandra E. , Lawlor Kate E. , Christensen Britt , Samson Andre L. , Vince James E. , Murphy James M. 
Published: November 2024

ABSTRACTInflammatory bowel disease (IBD) is a chronic condition caused by altered cytokine signaling, maladaptive immunity, dysbiosis, and intestinal barrier dysfunction. Patients with IBD receive therapy to correct these imbalances and achieve remission. However, most patients relapse, suggesting that pathological mechanisms persist during remission. Here, we show that excess epithelial cell death is an underlying feature of IBD that arises in patients in remission and on advanced therapy. Mechanistically, nascent inflammation reprograms epithelial cells into a macrophage-like state that promotes RIPK1-independent necroptotic signaling, then triggers iNOS-mediated mitochondrial apoptosis of absorptive epithelial cells and PUMA-mediated intestinal stem cell death. These findings reveal aberrant epithelial cell death signaling as a hallmark of IBD that occurs early in mucosal lesion development and persists despite current therapeutic approaches.One-Sentence SummaryEpithelial cell death is dysregulated in patients with inflammatory bowel disease.
Diffusion Monte Carlo calculation of fully heavy pentaquarks
Authors: Gordillo M. C. , Segovia J. , Alcaraz-Pelegrina J. M. 
Published: November 2024

Using a diffusion Monte Carlo algorithm, we calculated the spectra of all possible S-wave fully heavy pentaquarks within the framework of the quark model. Our aim was to compare the masses of different spin-color configurations corresponding to the same spatial wave function for each quark composition. In particular, we computed the masses of the configuration with the maximum number of undistinguishable quarks and those of all the possible baryon+meson splittings compatible with the total number of b’s and c’s for a given pentaquark. What we found was that, in all cases, compact baryon+meson configurations had lower masses than their more symmetric counterparts. Moreover, those “molecular” associations were unstable (with larger masses) that their infinitely separated noninteracting pieces except in the case of (ccb)(cb¯) and (bbc)(bc¯) pentaquarks. In any case, even for unstable arrangements, the excess masses are so small (∼20–60  MeV) as to make those compact molecular structures serious candidates to be experimentally detected.

Published by the American Physical Society
2024
Complex scalar dark matter in a new gauged U(1) symmetry with kinetic and direct mixings
Authors: Su Yu-Hang , Cai Chengfeng , Zeng Yu-Pan , Zhang Hong-Hao 
Published: November 2024

We propose a scalar dark matter model featuring a hidden gauge symmetry, denoted as U(1)X, with two complex scalars, Φ and S. In this framework, Φ spontaneously breaks the U(1)X gauge symmetry while S serves as a viable dark matter candidate. Particularly, the kinetic and direct mixings between the U(1)X and U(1)Y gauge groups provide a portal between dark matter and the Standard Model particles. These mixings offer a plausible explanation for the W boson mass anomaly observed by the Collider Detector at Fermilab Collaboration. We study the comprehensive phenomenological constraints of this model from colliders and dark matter detection experiments, including Z′ searches at the LHC, the 125 GeV Higgs boson measurements, the relic density of dark matter, and the indirect detection of dark matter annihilation. By randomly scanning the parameter space, we find that the regions where mZ′≳4750  GeV and mZ′≲4750  GeV for gx close to 1 remain viable and can be tested by future experiments.

Published by the American Physical Society
2024
Are discrete dimensions of corporate social responsibility disclosures global, regional or hybrid?
Authors: Sambharya Rakesh B. 
Published: November 2024

PurposeWe test whether six individual components of corporate social responsibility (CSR) disclosures in the form of environmental, employee, anticorruption, non-governmental organizations (NGOs), health and human rights are converging (global), diverging (regional) or crossverging (hybrid) on a sample of 335 multinational enterprises (MNEs) from 31 countries from three regions of the world: Europe, Anglo-Saxon cluster and Asia and the emerging markets.Design/methodology/approach We use an analysis of variance (ANOVA) and multivariate analysis of covariance (MANCOVA) analysis to test for differences between the six components of CSR on a sample of 335 MNEs from 310 countries for three regions of the world.Findings The effect of one-way ANOVA was significant for each of the six dependent variables separately on the differences between MNEs from the three regions. Taken together clearly the MANCOVA analysis indicates that there are signs of crossvergence between MNEs from Asia and the emerging markets and those from the Anglo-Saxon and European subsamples due to the hybrid nature of the findings.Research limitations/implications The results of the present study support the regional nature of CSR practices and disclosures and on many of the individual components CSR disclosures are a reflection of their home environments.Originality/valueThe study sheds light on the ongoing debate on whether CSR practices are converging, diverging or crossverging on a sample of 335 MNEs from the three regions of the world. Clear evidence of divergence was seen in that in all six dimensions of CSR, there were differences between MNEs from Europe and those from the Anglo-Saxon region reinforcing the implicit and explicit nature of the phenomena. There were significant differences between MNEs from Asia and emerging markets and Europe on four out of the six dimensions of CSR indicating mostly divergence. There were also significant differences between MNEs from Asia and emerging markets and those from the Anglo-Saxon region on three out of the six dimensions of CSR indicating the presence of divergence.
Energy transition governance in the ASEAN: current status and future prospects
Authors: Johnstone Injy 
Published: November 2024

PurposeA study of Association of Southeast Asian Nations (ASEAN) member states’ (AMS) norm entrepreneurial practices regarding the energy transition to reveal insights into its current and future governance.Design/methodology/approachUnderstanding why the notion of an energy transition has garnered support, and importantly, knowing what this means for the future requires reviewing the forces of norm entrepreneurship at play. By adopting a constructivist lens, this article undertakes a comparative analysis of the “modes of transmission” of the energy transition amongst AMS.FindingsASEAN nations have in many respects been at the vanguard of the energy transition. Yet the governments themselves demonstrate both norm entrepreneurial and antipreneurial characteristics. Notwithstanding this mix, there are extant opportunities for “intraconnection” – in the form of cooperation between AMS and “interconnection” – in the form of cooperation between AMS and other state and non-state actors.Practical implicationsThis article underscores the importance of partnerships in the energy transition: both in enabling AMS to meet their respective nationally determined contributions (NDCs) but also crucially in catalysing action amongst a broader swathe of countries.Originality/valueApplying the lens of norm entrepreneurship to the ASEAN context, including introducing novel conceptions of “conductive” practices that drive the energy transition and “resistive” practices that slow it down.
Bridging the implementation gap for climate mitigation in ASEAN: a comprehensive capacity-building framework
Authors: Gui Emi Minghui , Overland Indra , Suryadi Beni , Yurnaidi Zulfikar 
Published: November 2024

PurposeThe paper systematically examines the capacity building needs of energy and climate stakeholders in the Association of Southeast Asian Nations (ASEAN). It looks at conditions and opportunities for improvements in institutional, organisational, technological, innovation and financing capacities. This paper provides a guide to concrete capacity building programs and implementations to accelerate the implementation of National Determined Contributions (NDCs) and low-carbon energy transition in the ASEAN region.Design/methodology/approachThis paper proposes a comprehensive capacity-building framework, drawing on transition management theory and the interactive systems framework for capacity building. The assessment is based on interviews with representatives of the ministry responsible for energy policy and the ministry responsible for climate policy in each ASEAN country, as well as a survey among a broader set of Southeast Asian energy and climate experts from academia, think tanks and international development partners.FindingsThe paper identifies the priority areas for capacity building for each ASEAN country and the region as a whole. Each country has a unique set of needs and priorities. At the regional level, the widest capacity gaps were observed in institutional capacity, technical capacity, human resources capacity, financing capacity and the capacity to develop policy and legislation. Specific gaps for capacity building are discussed in delivering strategic areas of energy transition, such as electrification of transportation, development of the green supply chain, deploying renewable energy, energy efficiency, strengthening finance and investment and reducing dependencies on fossil fuels.Originality/valueThis paper helps fill the gap for detailed capacity needs analysis and facilitates long-term plans/strategies and their implementation. The insights help to increase ASEAN energy and climate stakeholders’ understanding of the interaction between energy and climate, therefore enhanced capability in developing more effective action maps and intervention points in achieving NDCs and sustainable development goals.
Servitization in the construction industry: a multiple case study from a value-adding perspective
Authors: Li Huimin , Zhao Zhichao , Cao Yongchao , Su Limin , Zhang Yafei , Zhao Jing 
Published: November 2024

Purpose
The purpose of this paper is to address the urgent need for transformation and upgrading in the construction industry amid the accelerating industrialization process. The focus is on understanding how value-added for construction enterprises can be achieved through servitization.

Design/methodology/approach
Grounded in the perspective of added value of construction enterprises, this paper uses a multicase analysis method, selecting four internationally renowned construction enterprises as the research subjects. The analysis investigates how these case study enterprises transition toward servitization and explores the trends in servitization in construction.

Findings
The research results indicate that servitization manifests in two aspects: contractor transition to servitization, and the shift of the construction industry toward integrated delivery. Furthermore, servitization in construction can achieve value-adding through three pathways: increasing product value, creating market demand and providing customized services.

Originality/value
This study contributes by providing insights into the manifestations of servitization and pathways for value-added. It provides a reference for the overall direction and basic strategy of servitization in construction.
Can indigenous peasants of Central Kalimantan live viably while conserving their forest lands?
Authors: Wibowo Wahyudi , Jagau Yanedi , Christianto Standy , Sulang Kusni 
Published: November 2024

Purpose
This study aims to investigate the feasibility of a community-based non-timber forest products (NTFP) enterprise, or CBNE, within the indigenous peasant communities in Manuhing Raya, Central Kalimantan, Indonesia. This includes assessing the potential for generating featured agricultural products from the region.

Design/methodology/approach
This is a descriptive qualitative study that integrates ethnographic methods into the SLA analysis, to investigate the feasibility of a CBNE approach within an indigenous peasant community. A number of 13 peasant groups, which comprise 251 members, from the seven villages in Manuhing Raya participated in the study.

Findings
The CBNE approach, when combined with One Village One Product method, is considered appropriate for the determination of featured agricultural products in a local community. In addition, the SLA analysis in this study contributes to identifying vulnerable aspects of CBNE development in the local communities, including weaknesses in social cohesion, leadership and entrepreneurial orientation.

Research limitations/implications
Efforts to preserve the rainforest lands in Central Kalimantan cannot be excluded from the involvement of the local peasant communities. The best livelihood strategy in this case is to adopt a CBNE approach. This shall be made possible by empowering the peasant communities to revive their local wisdom of Upun Pambelum, which means forest lands are their primary resources for life.

Practical implications
This study highlights the importance of using ethnographic investigation prior to the implementation of a CBNE development.

Originality/value
The implementation of the CBNE approach should take into account socioeconomic aspects of the local community, especially the presence of social cohesion, leadership roles and entrepreneurial orientations. In addition, the study demonstrates the potential of integrating traditional ecological knowledge with modern business models in CBNEs.
Value tensions and actionable knowledge in grassroots innovation: a study of invitational ambiguity and implementation challenges
Authors: Esposito Gabriella , De Bernardi Paola , Bertello Alberto , Vrontis Demetris 
Published: November 2024

Purpose
This research paper explores how grassroots innovation initiatives address and resolve the challenges of translating broad and inviting values such as sustainability, inclusiveness and aesthetics into practical and actionable structures. This study examines the tensions and difficulties projects face in operationalizing these values, revealing the gap between idealistic goals and real-world implementation. Moreover, this paper analyzes how role expectations and the concept of invitation ambiguity affect top down and bottom up approaches, offering insights for improving mechanisms to support grassroots innovations.

Design/methodology/approach
This study uses an exploratory qualitative methodology with an embedded case study design, focusing on the New European Bauhaus (NEB) and its award-winning projects. Data were collected through online self-assessment surveys, secondary data analysis, and semi-structured interviews with project owners and NEB Unit representatives.

Findings
The findings reveal significant challenges in translating broad and inviting values (sustainability, inclusion and aesthetics) into actionable outcomes for grassroots projects. Key issues include the need for clearer role definitions, tailored support, and adaptability. Conflicts between those values and a mismatch between expectations about stakeholders’ contributions highlight the need for designing more flexible and robust frameworks and robust frameworks.

Originality/value
This research explores the effects of invitational ambiguity within grassroots innovation, revealing how broad values ‐ like sustainability, inclusion and aesthetics ‐ are operationalized in real-world settings. By applying collective action theoretical frameworks to the unique case study of NEB projects, this study provides fresh insights into the dynamics between top-down European policies and bottom-up grassroots practices.