bluuebunny/crossref_metadata_2025_split huggingface dataset:
KIFC1 is activated by TCF-4 and promotes hepatocellular carcinoma pathogenesis by regulating HMGA1 transcriptional activity
Authors: Teng Kai , Wei Shi , Zhang Chi , Chen Jiewei , Chen Jinbin , Xiao Kanghua , Liu Jun , Dai Miaomiao , Guan Xinyuan , Yun Jingping , Xie Dan 
Published: July 2019

Abstract
Background
Kinesins play important roles in the development and progression of many human cancers. The functions and underlying mechanisms of kinesin family member C1 (KIFC1), a member of the kinesin-14 family, in the pathogenesis of hepatocellular carcinoma (HCC) have not been fully elucidated.

Methods
In this study, 168 HCC samples were first analyzed to examine the association between KIFC1 expression and patient clinicopathological features and prognosis. The role of KIFC1 in HCC cell proliferation and metastasis was investigated both in vivo and in vitro. The upstream regulation and downstream targets of KIFC1 were studied by qRT-PCR, western blotting, coimmunoprecipitation, chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays.

Results
KIFC1 was highly expressed in HCC tissues and positively associated with advanced stages and poor prognosis. KIFC1 knockdown suppressed HCC cell proliferation and invasion both in vitro and in vivo. Furthermore, KIFC1 knockdown decreased invadopodia formation and reduced epithelial-mesenchymal transition (EMT). HMGA1, an architectural transcriptional factor, was identified to interact with KIFC1. HMGA1 could bind to the promoters of Stat3, MMP2 and EMT-related genes and promote gene transcription. KIFC1 enhanced HMGA1 transcriptional activity and facilitated HCC proliferation and invasion. Moreover, KIFC1 was activated by TCF-4, and KIFC1 inhibition enhanced HCC cell sensitivity to paclitaxel.

Conclusions
Our findings suggest that KIFC1, activated by TCF-4, functions as an oncogene and promotes HCC pathogenesis through regulating HMGA1 transcriptional activity and that KIFC1 is a potential therapeutic target to enhance the paclitaxel sensitivity of HCC.
Limb ischemia in peripheral veno-arterial extracorporeal membrane oxygenation: a narrative review of incidence, prevention, monitoring, and treatment
Authors: Bonicolini Eleonora , Martucci Gennaro , Simons Jorik , Raffa Giuseppe M. , Spina Cristina , Lo Coco Valeria , Arcadipane Antonio , Pilato Michele , Lorusso Roberto 
Published: July 2019

AbstractVeno-arterial extracorporeal membrane oxygenation (V-A ECMO) is an increasingly adopted life-saving mechanical circulatory support for a number of potentially reversible or treatable cardiac diseases. It is also started as a bridge-to-transplantation/ventricular assist device in the case of unrecoverable cardiac or cardio-respiratory illness. In recent years, principally for non-post-cardiotomy shock, peripheral cannulation using the femoral vessels has been the approach of choice because it does not need the chest opening, can be quickly established, can be applied percutaneously, and is less likely to cause bleeding and infections than central cannulation. Peripheral ECMO, however, is characterized by a higher rate of vascular complications. The mechanisms of such adverse events are often multifactorial, including suboptimal arterial perfusion and hemodynamic instability due to the underlying disease, peripheral vascular disease, and placement of cannulas that nearly occlude the vessel. The effect of femoral artery damage and/or significant reduced limb perfusion can be devastating because limb ischemia can lead to compartment syndrome, requiring fasciotomy and, occasionally, even limb amputation, thereby negatively impacting hospital stay, long-term functional outcomes, and survival. Data on this topic are highly fragmentary, and there are no clear-cut recommendations. Accordingly, the strategies adopted to cope with this complication vary a great deal, ranging from preventive placement of antegrade distal perfusion cannulas to rescue interventions and vascular surgery after the complication has manifested.This review aims to provide a comprehensive overview of limb ischemia during femoral cannulation for VA-ECMO in adults, focusing on incidence, tools for early diagnosis, risk factors, and preventive and treating strategies.
Systemic chemotherapy plus cetuximab after complete surgery in the treatment of isolated colorectal peritoneal carcinoma: COCHISE phase II clinical trial
Authors: Evrard Serge , Desolneux Grégoire , Bellera Carine , Esnaud Thomas , Bécouarn Yves , Collet Denis , Chafai Najim , Marchal Francois , Cany Laurent , Lermite Emilie , Rivoire Michel , Mathoulin-Pélissier Simone 
Published: July 2019

Abstract
Objective
The primary objective of this non-randomised phase II study was to evaluate the combination of systemic chemotherapy plus cetuximab after complete cytoreductive surgery (CCS) for treatment of isolated colorectal peritoneal carcinoma (CRPC). This multicentre, prospective phase II clinical trial was conducted in seven national cancer referral centres, however research published during study recruitment indicated cetuximab treatment as ineffective in patients with mutated KRAS genes, leading to an additional exclusion criterion to the current protocol, excluding patients with mutated KRAS genes. This significantly impacted recruitment and the study did not achieve the necessary recruitment of 46 patients.

Results
Fourteen patients underwent CCS and were included in the study, however one did not provide informed consent and another received only one cycle of chemotherapy leading to 12 patients in the per protocol population for analysis. Adjuvant Folfox Cetuximab was administered when CCS was achieved for patients > 18 years with histologically proven CRPC and no other metastatic disease (liver, lungs, lymphadenopathy, etc.). CRPC median index was 5.00 (range: 1–17). Median PFS was 12.3 months [95% CI (3.7–28.2)] with 8.3% [95% CI (0.5–31.1)] and 0% PFS at 3 and 5 years respectively. Median OS was 43.4 months [95% CI (16.8–60)].
Trial registration Clinical Trials NCT00766142, October 3, 2008. Retrospectively registered
Review of design optimization of fluid machinery: applying computational fluid dynamics and numerical optimization
Authors: Chaudhry Qasim Ali , Al-Mdallal Qasem M. 
Published: July 2019

Book detailsAuthors: Kwang-Yong Kim, Abdus Samad, Ernesto BeniniTitle: Design Optimization of Fluid Machinery: Applying Computational Fluid Dynamics and Numerical OptimizationISBN: 9781119188308 (ePub)Published by: JohnWiley & Sons Singapore Pte. Ltd; 2019
Evaluation of one-point fixation for zygomaticomaxillary complex fractures using a three-dimensional photogrammetric analysis
Authors: Kim Se Young , Nam Seung Min , Park Eun Soo , Kim Yong Bae 
Published: July 2019

Background The goal of treatment for zygomaticomaxillary (ZM) complex (ZMC) fractures is to achieve stability and restore aesthetic appearance through three-dimensional reduction and rigid fixation. The purpose of this study was to evaluate the stability and aesthetic appearance outcomes of one-point fixation using a three-dimensional photogrammetric analysis. Methods From March 2014 to December 2014, 34 patients with ZMC fractures were treated by one-point fixation in the ZM buttress using unsintered hydroxyapatite (u-HA)/poly-L-lactide (PLLA) plates. Differences in soft tissue inter-malar height between the fractured side and unfractured sides were evaluated using photogrammetric analysis with a three-dimensional camera (Morphius®) at the preoperative and 1 week, 1 and 3 months after surgery. The differences in bony inter-malar height between the fractured and unfractured sides were evaluated using computer tomography at the pre-operative and 6 months after surgery. The paired t-test was used to compare differences in malar height. Results Six months after surgery, 34 patients achieved satisfactory bony stability and symmetric malar appearances. Comparisons of differences in soft-tissue inter-malar height revealed statistically significant differences between the pre-operative period and 1 week and 1 month after surgery ( p < .01). There was no statistically significant difference between 1 and 3 months after surgery. Comparison of differences in bony inter-malar height revealed a statistically significant difference between before and 6 months after surgery ( p < .01). Conclusions When we conducted a three-dimensional photogrammetric analysis, although it has restricted surgical indications, one-point fixation of the ZM buttress using an u-HA / PLLA plate yielded reliable, satisfactory, and safe clinical results in patients with ZMC fractures. Clinical question / level of evidence Therapeutic, III.
Archaeometric characterization of Byzantine pottery from Păcuiul lui Soare
Authors: Bugoi Roxana , Talmaţchi Cristina , Haitǎ Constantin , Ceccato Daniele 
Published: July 2019

Abstract
Archaeometric investigations using OM (optical microscopy) and micro-PIXE (particle induced X-ray emission) were performed on 45 ceramic shards unearthed in archaeological excavations at Păcuiul lui Soare (southeastern Romania) and dated to the eleventh century AD. This study aimed to get clues about the raw materials and manufacturing techniques used by the potters from the Lower Danube area during the Byzantine period. The analyzed ceramic fragments were selected according to stylistic and archaeological criteria, trying to cover the entire palette of potteries discovered at this site. OM detailed the characteristics of the fabric (texture, microstructure and porosity), mineralogy, surface treatments and firing of the shards. Principal component analysis (PCA) of the PIXE data highlighted two main categories of shards with distinct compositional signatures, separated mainly by their aluminum and calcium content. Micro-PIXE maps of the interfaces between the glaze and the ceramic body showed that the green glaze is rich in lead oxide compared to the underlying ceramic body. The results of these investigations were compared to the ones previously obtained on coeval potteries from other Byzantine archaeological sites, i.e. Hârşova and Oltina, trying to get some hints about the consumption and circulation of pottery in the Lower Danube region at the beginning of the second millennium AD.
Recent Publications on the Art and Archaeology of Kucha
Authors: Lee Sonya S. 
Published: July 2019

Abstract
Kucha was one of the major political powers and cultural centers along the ancient Silk Road, home to a great number of Buddhist cave temples that have survived from the time of their creation sometime between the third century and the eighth. Although they are not as well-known as their counterparts in Dunhuang, the complexes at Kizil and Kumutra, among others, have preserved equally invaluable material evidence of the vibrant interchange of goods, ideas, and cultural practices that took place across the entire region in the first millennium. These sites represent the crucial link with the artistic traditions of Gandhara, India, and Persia in explicating the Chinese adaptation of a complex, foreign visual culture through the introduction of Buddhism. This essay reviews a number of significant publications on the art and archaeology of Kucha that have appeared in the past decade. Marking one of the notable trends in Asian studies today, the remarkable growth in Kucha scholarship has been facilitated in one way or another by the opening of China's Xinjiang Uighur Autonomous Region to the outside world. The review focuses on the compilations of source materials, reception and collection histories, and interpretative studies of source materials, examining each of these three areas within their proper historical and historiographical contexts. An extensive review of Archaeological and Visual Sources of Meditation in the Ancient Monasteries of Kuča (2015) by Angela F. Howard and Giuseppe Vignato appears in the last section of the essay.
Frequency and impact of incidental findings on computed tomography during work-up for transcatheter aortic valve implantation: single centre experience and review of the literature
Authors: Hinton Jonathan , Gough Sam , Ahmed Hanad , Gabara Lavinia , Rawlins John , Calver Alison , Shah Benoy N , Rakhit Dhrubo , Shambrook James , Harden Stephen , Peebles Charles , Abbas Ausami , Curzen Nick 
Published: July 2019

Objective:
To assess the frequency and impact of incidental findings (IF) on CT during work-up for transcatheter aortic valve intervention (TAVI).

Methods:
A consecutive cohort of patients referred for consideration of TAVI who underwent a CT scan between 2009 and 2018 were studied retrospectively. CT reports were reviewed for the presence of IFs and categorised based upon their clinical significance: (a) insignificant—findings that did not require specific treatment or follow-up; (b) intermediate—findings that did not impact on the decision-making process but required follow-up; (c) significant—findings that either required urgent investigation or meant that TAVI was clinically inappropriate.

Results:
A total of 652 patients were included, whose median age was 82 years. One or more insignificant IF was found in 95.6% of patients. Intermediate IFs were documented in 5.4%. 91 (14%) patients had at least one significant IF. These included possible malignancy in 67 (74%). The ultimate decision to offer aortic valve intervention was only changed by the presence of an IF in 3.5% of cases.

Conclusion:
Clinically significant IFs are detected in more than 1 in 10 of patients undergoing CT as part of a TAVI work-up, although just over half of these patients still receive aortic valve intervention.

Advances in knowledge:
This study is the largest UK cohort, which, when combined with a review of existing literature, provides a clear picture of the frequency and clinical impact of IFs found at CT for TAVI assessment.
A co-culture model of the bovine alveolus
Authors: Lee Diane , Chambers Mark 
Published: July 2019

The epithelial lining of the lung is often the first point of interaction between the host and inhaled pathogens, allergens and medications. Epithelial cells are therefore the main focus of studies which aim to shed light on host-pathogen interactions, to dissect the mechanisms of local host immunity and study toxicology. If these studies are not to be conducted exclusively in vivo, it is imperative that in vitro models are developed with a high in vitro- in vivo correlation. We describe here a co-culture model of the bovine alveolus, designed to overcome some of the limitations encountered with mono-culture and live animal models. Our system includes bovine pulmonary arterial endothelial cells (BPAECs) seeded onto a permeable membrane in 24 well Transwell format. The BPAECs are overlaid with immortalised bovine alveolar type II epithelial cells and cultured at air-liquid interface for 14 days before use; in our case to study host-mycobacterial interactions. Characterisation of novel cell lines and the co-culture model have provided compelling evidence that immortalised bovine alveolar type II cells are an authentic substitute for primary alveolar type II cells and their co-culture with BPAECs provides a physiologically relevant in vitro model of the bovine alveolus. The co-culture model may be used to study dynamic intracellular and extracellular host-pathogen interactions, using proteomics, genomics, live cell imaging, in-cell ELISA and confocal microscopy. The model presented in this article enables other researchers to establish an in vitro model of the bovine alveolus that is easy to set up, malleable and serves as a comparable alternative to in vivo models, whilst allowing study of early host-pathogen interactions, currently not feasible in vivo. The model therefore achieves one of the 3Rs objectives in that it replaces the use of animals in research of bovine respiratory diseases.
The diversity of Anopheles blood feeding patterns suggests different malaria protection strategies in different localities
Authors: Chakim Irfanul , Pumpaibool Tepanata 
Published: July 2019

Background: Malaria is a significant health burden for many countries worldwide. Insecticide-treated bed nets and mosquito repellent are considered effective methods for preventing Anopheles bites. However, changes in the biological properties of the vector have led to a reduction in their effectiveness. Most published studies have only investigated the human population factor, not the dynamics of vector behavior. Therefore, this study aims to investigate the importance of primary vector activity for selecting an appropriate malaria protection strategy. Methods: Initially, active case detection (ACD) was carried out in western and eastern parts of Indonesia, Jambi and Sumba, to confirm their endemicity level. According to the 2016 national health report of Indonesia, Jambi has an annual parasite index (API) of 0.14 and Sumba has an API of 5.41. A series of entomological observations were carried out to compare the biting activity of Anopheles vector in two localities, with a total of 216 houses and catchers (108 in each study site). Results: The results indicated that endemicity at the sub-district level is higher than that at the provincial level. Only Anopheles balabacensi was found to be exophagic. Multiple comparisons found different biting times between the sites, suggesting that early evening (18.00-20.00) is most likely to be the time when mosquitos transmit the Plasmodium parasite in Jambi, while during sleeping hours (21.00-01.00) is the peak biting time of Anopheles mosquitos in Sumba. Conclusions: The study demonstrates the importance of Anopheles species blood feeding patterns in selecting an appropriate malaria protection strategy.
Levels of mercury in Nile tilapia (Oreochromis niloticus), water, and sediment in the Migori gold mining belt, Kenya, and the potential ramifications to human health
Authors: Kola Samwel , Kanja Laetitia Wakonyu , Mbaria James Mucunu , Maina Joyce Gichiku , Okumu Mitchel Otieno 
Published: July 2019

Background: Understanding mercury levels in gold mining areas where locals consume fish is important in evaluating the risk to the population. This study determined the levels of total mercury (T-Hg) in Nile tilapia, water, and sediment in the Migori gold mining belt and the potential risk to human health. Methods: Water, sediment, and fish were sampled from 10 sites in Rongo and Nyatike and analyzed for T-Hg using cold vapor atomic absorption spectroscopy. Geo-accumulation index (IGeo) was used to evaluate sediment quality, and the estimated daily intake of fish per meal (EDIm), the target hazard quotient (THQ), and the maximum allowable fish consumption rate (CRmw) were calculated as health risk indices. Results: Sediment from 6 sites were moderately polluted with T-Hg, while 2 sites were strongly polluted. Water from all the sites had T-Hg levels higher than the FAO recommended level for surface water. About 78% (38/49) of all tissues sampled had T-Hg levels above the 0.5 µg/g limit for consumption by the general human population. About 31% (15/49) of muscle tissues had T-Hg levels above the 0.5 µg/g limit, while 88% (43/49) of brain, 69% (34/49) of liver, and 69% (34/49) of muscle tissues had T-Hg levels above the 0.2 µg/g limit for consumption by at-risk groups. There were positive correlations between T-Hg levels in brain, muscle and pond sediment, T-Hg levels in brain and water pH and temperature, and negative correlations between T-Hg levels in brain and levels in water, T-Hg levels in brain and weight of fish. EDIm and THQ ranged from 2.43-15.84 µg/g and 24.3-158.4 µg/g wet weight respectively while CRmw ranged from 1-4 fish. Conclusions: Consumption of Nile tilapia from the studied area carries a significant risk of Hg exposure in frequent fish-eaters, pregnant women, and developing children, but is safe for the general population.
Activation of Small Organic Molecules on Ti2+-Rich TiO2 Surfaces: Deoxygenation vs. C–C Coupling
Authors: Kräuter Jessica , Mohrhusen Lars , Thiedemann Tim , Willms Michael , Al-Shamery Katharina 
Published: July 2019

Abstract
Rutile TiO2 is an important model system for understanding the adsorption and conversion of molecules on transition metal oxide catalysts. In the last decades, point defects, such as oxygen vacancies and Ti3+ interstitials, exhibited an important influence on the reaction of oxygen and oxygen-containing molecules on titania surfaces. In brief, partially reduced TiO2 containing a significant amount of Ti3+ is often more active for the conversion of such molecules. In this study, we investigate an even higher reduced surface prepared by argon ion bombardment of a rutile TiO2 (110) single crystal. By X-ray photoelectron spectroscopy we show that, besides Ti4+, this surface is almost equally dominated by Ti3+ and Ti2+. To probe the reactivity of these highly reduced surfaces, we have adsorbed two different classes of oxygen-containing molecules and utilized temperature programmed reaction spectroscopy to investigate the conversion. While alcohols (in this case methanol) already show a defect-dependent partial conversion in a deoxygenation reaction on the (stochiometric or slightly reduced) rutile TiO2 (110) surface, ketones (e.g. acetone) are usually not converted on the rutile TiO2 (110) surface independent on the bulk defect density. Here, we present a nearly full conversion for both molecules via deoxygenation reactions and reductive C–C coupling, forming different hydrocarbons at different temperatures between 375 K and 640 K on the sputtered Ti2+ rich surface.
Impact of Two High-Volume Set Configuration Workouts on Resistance Training Outcomes in Recreationally Trained Men
Authors: Karsten Bettina , Fu Yue (Leon) , Larumbe-Zabala Eneko , Seijo Marco , Naclerio Fernando 
Published: July 2019

Abstract
Karsten, DB, Fu, Y, Larumbe-Zabala, DE, Seijo, DM, and Naclerio, DF. Impact of two high-volume set configuration workouts on resistance training outcomes in recreationally trained men. J Strength Cond Res 35(2S): S136–S143, 2021—This study compared the effects of 2 weekly equalized by volume, loading zone and frequency resistance training designs using repetition to failure (RTF) or not to failure (NTF), on body composition, strength, and mechanical power. Based on individual baseline maximal strength, 18 recreationally resistance-trained men were pair-matched and consequently randomly assigned to an RTF (n = 9) or an NTF (n = 9) protocol. Subjects trained for 6 weeks using 2 different routines performed once per week (2 workouts per week). The RTF protocol comprised 4 sets of 10 repetitions per exercise with 2-minute rest and the NTF involved 8 sets of 5 repetitions per exercise with 1-minute rest. Subjects were tested pre- and post-intervention for maximal strength, upper- and lower-body power, fat-free mass, limb circumferences, and muscle thickness. Compared to baseline, both groups improved (p < 0.01) the maximal loads lifted in the bench press (RTF +9.44 ± 3.00 kg; NTF +7.22 ± 4.41 kg) and the squat (RTF +9.44 ± 4.64 kg; NTF +11.1 ± 10.33 kg) exercises, but only the NTF group increased (p < 0.05) upper-body power (+15.73 ± 12.59 W). Conversely, only the RTF group showed significant (p < 0.05) increase of the elbow flexors (+3.44 ± 5.11 mm) and vastus medialis (+3.28 ± 2.32 mm) thickness, whereas both groups enhanced anterior deltoid thickness (RTF +1.84 ± 1.68 mm, p < 0.05; NTF +2.76 ± 2.63 mm, p < 0.01). Although both training strategies improved strength, the RTF group elicited superior hypertrophic outcomes, whereas the NTF protocol resulted in more favorable improvements for upper-body power.