bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using Standardized Checklists Increase the Completion Rate of Critical Actions in an Evacuation from the Operating Room: A Randomized Controlled Simulation Study
Authors: Acar Yahya A. , Mehta Neil , Rich Mary-Ann , Yilmaz Banu Karakus , Careskey Matthew , Generoso Jose , Fidler Richard , Hirsch Jan 
Published: August 2019

AbstractIntroduction:Hospital evacuations of patients with special needs are extremely challenging, and it is difficult to train hospital workers for this rare event.Hypothesis/Problem:Researchers developed an in-situ simulation study investigating the effect of standardized checklists on the evacuation of a patient under general anesthesia from the operating room (OR) and hypothesized that checklists would improve the completion rate of critical actions and decrease evacuation time.Methods:A vertical evacuation of the high-fidelity manikin (SimMan3G; Laerdal Inc.; Norway) was performed and participants were asked to lead the team and evacuate the manikin to the ground floor after a mock fire alarm. Participants were randomized to two groups: one was given an evacuation checklist (checklist group [CG]) and the other was not (non-checklist group [NCG]). A total of 19 scenarios were run with 28 participants.Results:Mean scenario time, preparation phase of evacuation, and time to transport the manikin down the stairs did not differ significantly between groups (P = .369, .462, and .935, respectively). The CG group showed significantly better performance of critical actions, including securing the airway, taking additional drug supplies, and taking additional equipment supplies (P = .047, .001, and .001, respectively). In the post-evacuation surveys, 27 out of 28 participants agreed that checklists would improve the evacuation process in a real event.Conclusion:Standardized checklists increase the completion rate of pre-defined critical actions in evacuations out of the OR, which likely improves patient safety. Checklist use did not have a significant effect on total evacuation time.
A New Inverse Phase Speed Spectrum of Nonlinear Gravity Wind Waves
Authors: Björkqvist Jan‐Victor , Pettersson Heidi , Drennan William M. , Kahma Kimmo K. 
Published: August 2019

AbstractThe rear face of the wave spectrum is described by an equilibrium and a saturation subrange. Although accurate information about these ranges are highly relevant for wave modeling and many practical applications, there have been inconsistencies between results originating from temporal and spatial measurements. These discrepancies have been explained by the Doppler shift and the harmonics of nonlinear waves. We present high‐frequency wave measurements from the Baltic Sea gathered with R/V Aranda using a wave staff array, which provided directional frequency‐wavenumber data. In addition to the traditional wavenumber and frequency spectra, F(k) and S(ω), we also define a new spectrum that is a function of the inverse phase speed. We denote this spectrum Q(ν), where ν=kω−1. The properties of this Q‐spectrum were studied using data from four different sites. A strongly forced fetch‐limited case showed an equilibrium‐to‐saturation transition in the Q‐spectrum, with less variations in the equilibrium constants compared to the frequency spectra. The transition to a saturation regime happened around Uν=3 in all spectra where an equilibrium range was identified. Most duration‐limited spectra had no equilibrium range in the inverse phase speed domain. The absence of an equilibrium range was consistent with the wavenumber domain, but the frequency spectra still showed an apparent equilibrium subrange extending to ωU/g=5. The consistency of the saturation ranges between the Q‐spectrum and the wavenumber spectrum indicate a weak Doppler shift effect. We deduced that the main factor distorting the frequency spectra was wave nonlinearities.
Seasonal Dynamics of Organic Matter Composition and Its Effects on Suspended Sediment Flocculation in River Water
Authors: Lee B.J. , Kim J. , Hur J. , Choi I. H. , Toorman E. A. , Fettweis M. , Choi J. W. 
Published: August 2019

AbstractOrganic matter (OM) and suspended sediment are abundant, and interact with each other, in rivers and lakes. OM is usually adsorbed by suspended sediment and causes either particle stabilization or flocculation. In this study, the OM composition and suspended sediment flocculation potential of river water were regularly measured throughout the year 2016. The OM composition of the river water samples was measured with a liquid chromatography‐organic carbon detection system and fluorescence excitation‐emission matrix spectroscopy, and the flocculation potential was measured in a standard jar test experiment. Results from the OM analyses and flocculation potential tests, in association with a multivariate data analysis, demonstrated that the OM composition and flocculation potential of the river water were dynamic under different meteorological, hydrological, ecological, and anthropogenic conditions and closely correlated with each other. Dry seasons with low rainfall and water discharge induced a lacustrine condition and led to the OM composition being more aquagenic and flocculation‐favorable. The most favorable condition for the enhancement of flocculation was during algae bloom and associated with the production of biopolymers from algae. In contrast, rainy seasons were advantageous for stabilization of suspended sediment because of excessive transport of terrigenous humic substances from catchment areas into the river. Such terrigenous humic substances enhanced stabilization by creating enhanced electrostatic repulsion via adsorption onto the sediment surface. Findings from this research provide a better insight into the highly complex behaviors of and interactions between OM and suspended sediment in natural water environments.
Observational Evidence for Two Modes of Coupling Between Sea Surface Temperatures, Tropospheric Temperature Profile, and Shortwave Cloud Radiative Effect in the Tropics
Authors: Fueglistaler S. 
Published: August 2019

AbstractTropical average shortwave cloud radiative effect (SWCRE) anomalies observed by CERES/EBAF v4 are explained by observed average sea surface temperature (
) and the difference between the warmest 30% where deep convection occurs and
). Observed tropospheric temperatures show variations in boundary layer capping strength over time consistent with the evolution of SST#. The CERES/EBAF v4 data confirm that associated cloud fraction changes over the colder waters dominate SWCRE. This observational evidence for the “pattern effect” noted in General Circulation Model simulations suggests that SST# captures much of this effect. The observed sensitivities (dSWCRE/d
W·m−2·K−1, dSWCRE/dSST#≈−4.8W·m−2·K−1) largely reflect El Niño–Southern Oscillation. As El Niño develops,
increases and SST# decreases (both increasing SWCRE). Only after the El Niño peak, SST# increases and SWCRE decreases. SST# is also relevant for the tropical temperature trend profile controversy and the discrepancy between observed and modeled equatorial Pacific SST trends. Causality and implications for future climates are discussed.
Arctic Ocean Response to Greenland Sea Wind Anomalies in a Suite of Model Simulations
Authors: Muilwijk Morven , Ilicak Mehmet , Cornish Sam B. , Danilov Sergey , Gelderloos Renske , Gerdes Rüdiger , Haid Verena , Haine Thomas W. N. , Johnson Helen L. , Kostov Yavor , Kovács Tamás , Lique Camille , Marson Juliana M. , Myers Paul G. , Scott Jeffery , Smedsrud Lars H. , Talandier Claude , Wang Qiang 
Published: August 2019

AbstractMultimodel Arctic Ocean “climate response function” experiments are analyzed in order to explore the effects of anomalous wind forcing over the Greenland Sea (GS) on poleward ocean heat transport, Atlantic Water (AW) pathways, and the extent of Arctic sea ice. Particular emphasis is placed on the sensitivity of the AW circulation to anomalously strong or weak GS winds in relation to natural variability, the latter manifested as part of the North Atlantic Oscillation. We find that anomalously strong (weak) GS wind forcing, comparable in strength to a strong positive (negative) North Atlantic Oscillation index, results in an intensification (weakening) of the poleward AW flow, extending from south of the North Atlantic Subpolar Gyre, through the Nordic Seas, and all the way into the Canadian Basin. Reconstructions made utilizing the calculated climate response functions explain ∼50% of the simulated AW flow variance; this is the proportion of variability that can be explained by GS wind forcing. In the Barents and Kara Seas, there is a clear relationship between the wind‐driven anomalous AW inflow and the sea ice extent. Most of the anomalous AW heat is lost to the atmosphere, and loss of sea ice in the Barents Sea results in even more heat loss to the atmosphere, and thus effective ocean cooling. Release of passive tracers in a subset of the suite of models reveals differences in circulation patterns and shows that the flow of AW in the Arctic Ocean is highly dependent on the wind stress in the Nordic Seas.
Avenanthramide A triggers potent ROS-mediated anti-tumor effects in colorectal cancer by directly targeting DDX3
Authors: Fu Rong , Yang Peng , Li Zongwei , Liu Wen , Amin Sajid , Li Zhuoyu 
Published: August 2019

AbstractColorectal cancer (CRC) is a common malignant gastrointestinal tumor with high mortality worldwide. Drug resistance and cytotoxicity to normal cells are the main causes of chemotherapeutic treatment failure in CRC. Therefore, extracting the bioactive compounds from natural products with anti-carcinogenic activity and minimal side-effects is a promising strategy against CRC. The present study aims to evaluate the anti-carcinogenic properties of avenanthramides (AVNs) extracted from oats bran and clarify the underlying molecular mechanisms. We demonstrated that AVNs treatment suppressed mitochondrial bioenergetic generation, resulting in mitochondrial swelling and increased reactive oxygen species (ROS) production. Further study indicated that AVNs treatment significantly reduced DDX3 expression, an oncogenic RNA helicase highly expressed in human CRC tissues. DDX3 overexpression reversed the ROS-mediated CRC apoptosis induced by AVNs. Of note, we identified Avenanthramide A (AVN A) as the effective ingredient in AVNs extracts. AVN A blocked the ATPase activity of DDX3 and induced its degradation by directly binding to the Arg287 and Arg294 residues in DDX3. In conclusion, these innovative findings highlight that AVNs extracts, in particular its bioactive compound AVN A may crack the current hurdles in the way of CRC treatment.
Persistent accelerations disentangle Lagrangian turbulence
Authors: Bentkamp Lukas , Lalescu Cristian C. , Wilczek Michael 
Published: August 2019

AbstractParticles in turbulence frequently encounter extreme accelerations between extended periods of quiescence. The occurrence of extreme events is closely related to the intermittent spatial distribution of intense flow structures such as vorticity filaments. This mixed history of flow conditions leads to very complex particle statistics with a pronounced scale dependence, which presents one of the major challenges on the way to a non-equilibrium statistical mechanics of turbulence. Here, we introduce the notion of persistent Lagrangian acceleration, quantified by the squared particle acceleration coarse-grained over a viscous time scale. Conditioning Lagrangian particle data from simulations on this coarse-grained acceleration, we find remarkably simple, close-to-Gaussian statistics for a range of Reynolds numbers. This opens the possibility to decompose the complex particle statistics into much simpler sub-ensembles. Based on this observation, we develop a comprehensive theoretical framework for Lagrangian single-particle statistics that captures the acceleration, velocity increments as well as single-particle dispersion.
Enantioselective benzylic C–H arylation via photoredox and nickel dual catalysis
Authors: Cheng Xiaokai , Lu Huangzhe , Lu Zhan 
Published: August 2019

AbstractThe asymmetric cross-coupling reaction is developed as a straightforward strategy toward 1,1-diaryl alkanes, which are a key skeleton in a series of natural products and bioactive molecules in recent years. Here we report an enantioselective benzylic C(sp3)−H bond arylation via photoredox/nickel dual catalysis. Sterically hindered chiral biimidazoline ligands are designed for this asymmetric cross-coupling reaction. Readily available alkyl benzenes and aryl bromides with various functional groups tolerance can be easily and directly transferred to useful chiral 1,1-diaryl alkanes including pharmaceutical intermediates and bioactive molecules. This reaction proceeds smoothly under mild conditions without the use of external redox reagents.
Visualisation of dCas9 target search in vivo using an open-microscopy framework
Authors: Martens Koen J. A. , van Beljouw Sam P. B. , van der Els Simon , Vink Jochem N. A. , Baas Sander , Vogelaar George A. , Brouns Stan J. J. , van Baarlen Peter , Kleerebezem Michiel , Hohlbein Johannes 
Published: August 2019

AbstractCRISPR-Cas9 is widely used in genomic editing, but the kinetics of target search and its relation to the cellular concentration of Cas9 have remained elusive. Effective target search requires constant screening of the protospacer adjacent motif (PAM) and a 30 ms upper limit for screening was recently found. To further quantify the rapid switching between DNA-bound and freely-diffusing states of dCas9, we developed an open-microscopy framework, the miCube, and introduce Monte-Carlo diffusion distribution analysis (MC-DDA). Our analysis reveals that dCas9 is screening PAMs 40% of the time in Gram-positive Lactoccous lactis, averaging 17 ± 4 ms per binding event. Using heterogeneous dCas9 expression, we determine the number of cellular target-containing plasmids and derive the copy number dependent Cas9 cleavage. Furthermore, we show that dCas9 is not irreversibly bound to target sites but can still interfere with plasmid replication. Taken together, our quantitative data facilitates further optimization of the CRISPR-Cas toolbox.
SNARE protein SEC22B regulates early embryonic development
Authors: Wu Shin-Rong J. , Khoriaty Rami , Kim Stephanie H. , O’Shea K. Sue , Zhu Guojing , Hoenerhoff Mark , Zajac Cynthia , Oravecz-Wilson Katherine , Toubai Tomomi , Sun Yaping , Ginsburg David , Reddy Pavan 
Published: August 2019

AbstractThe highly conserved SNARE protein SEC22B mediates diverse and critical functions, including phagocytosis, cell growth, autophagy, and protein secretion. However, these characterizations have thus far been limited to in vitro work. Here, we expand our understanding of the role Sec22b plays in vivo. We utilized Cre-Lox mice to delete Sec22b in three tissue compartments. With a germline deletion of Sec22b, we observed embryonic death at E8.5. Hematopoietic/endothelial cell deletion of Sec22b also resulted in in utero death. Notably, mice with Sec22b deletion in CD11c-expressing cells of the hematopoietic system survive to adulthood. These data demonstrate Sec22b contributes to early embryogenesis through activity both in hematopoietic/endothelial tissues as well as in other tissues yet to be defined.
Gene-environment interaction counterbalances social impairment in mouse models of autism
Authors: Kim Ji-Woon , Park Kwanghoon , Kang Ri Jin , Gonzales Edson Luck , Oh Hyun Ah , Seung Hana , Ko Mee Jung , Cheong Jae Hoon , Chung ChiHye , Shin Chan Young 
Published: August 2019

AbstractAutism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by social communication deficits and repetitive/restricted behaviors. Although gene-environment interactions may explain the heterogeneous etiology of ASD, it is still largely unknown how the gene-environment interaction affects behavioral symptoms and pathophysiology in ASD. To address these questions, we used Cntnap2 knockout mice (genetic factor, G) exposed to valproic acid during embryonic development (environmental factor, E) as a gene-environment interaction (G × E) model. Paradoxically, the social deficits observed in the respective G and E models were improved in the G × E model; however, the high seizure susceptibility was more severe in the G × E -model than in the G and E models. Repetitive self-grooming and hyperactivity did not differ among the three models. The amplitudes of miniature excitatory postsynaptic currents in layer 2/3 pyramidal neurons of the medial prefrontal cortex were aberrant and similar in the G × E model when compared to the control group. Our findings suggest that the interaction of two risk factors does not always aggravate ASD symptoms but can also alleviate them, which may be key to understanding individual differences in behavioral phenotypes and symptom intensity.
Paxillin S273 Phosphorylation Regulates Adhesion Dynamics and Cell Migration through a Common Protein Complex with PAK1 and βPIX
Authors: Rajah Abira , Boudreau Colton G. , Ilie Alina , Wee Tse-Luen , Tang Kaixi , Borisov Aleksandar Z. , Orlowski John , Brown Claire M. 
Published: August 2019

AbstractCell migration is an important biological phenomenon involved in many homeostatic and aberrant physiological processes. Phosphorylation of the focal adhesion adaptor protein, paxillin, on serine 273 (S273) has been implicated as a key regulator of cell migration. Here, it is shown that phosphorylation on paxillin S273 leads to highly migratory cells with small dynamic adhesions. Adhesions at protrusive edges of the cell were more dynamic than adhesions at retracting edges. Temporal image correlation microscopy revealed that these dynamic adhesions undergo rapid binding of paxillin, PAK1 and βPIX. We identified membrane proximal adhesion subdomains in protrusive regions of the cell that show rapid protein binding that is dependent on paxillin S273 phosphorylation, PAK1 kinase activity and phosphatases. These dynamic adhesion subdomains corresponded to regions of the adhesion that also show co-binding of paxillin/PAK1 and paxillin/βPIX complexes. It is likely that parts of individual adhesions are more dynamic while others are less dynamic due to their association with the actin cytoskeleton. Variable adhesion and binding dynamics are regulated via differential paxillin S273 phosphorylation across the cell and within adhesions and are required for regulated cell migration. Dysregulation through phosphomutants, PAK1-KD or βPIX mutants resulted in large stable adhesions, long protein binding times and slow cell migration. Dysregulation through phosphomimics or PAK1-CA led to small dynamic adhesions and rapid cell migration reminiscent of highly migratory cancer cells. Thus, phosphorylation of paxillin S273 is a key regulator of cell migration through recruitment of βPIX and PAK1 to sites of adhesion.
10 kHz SCS therapy for chronic pain, effects on opioid usage: Post hoc analysis of data from two prospective studies
Authors: Al-Kaisy Adnan , Van Buyten Jean-Pierre , Carganillo Roy , Caraway David , Gliner Bradford , Subbaroyan Jeyakumar , Panwar Catherine , Rotte Anand , Amirdelfan Kasra , Kapural Leonardo 
Published: August 2019

AbstractChronic pain, including chronic low back and leg pain are prominent causes of disability worldwide. While patient management aims to reduce pain and improve daily function, prescription of opioids remains widespread despite significant adverse effects. This study pooled data from two large prospective trials on 10 kHz spinal cord stimulation (10 kHz SCS) in subjects with chronic low back pain and/or leg pain and performed post hoc analysis on changes in opioid dosage 12 months post 10 kHz SCS treatment. Patient-reported back and leg pain using the visual analog scale (VAS) and opioid dose (milligrams morphine equivalent/day, MME/day) were compared at 12 months post-10 kHz SCS therapy to baseline. Results showed that in the combined dataset, 39.3% of subjects were taking >90 MME dose of opioids at baseline compared to 23.0% at 12 months post-10 kHz SCS therapy (p = 0.007). The average dose of opioids in >90 MME group was significantly reduced by 46% following 10 kHz SCS therapy (p < 0.001), which was paralleled by significant pain relief (P < 0.001). In conclusion, current analysis demonstrates the benefits of 10 kHz SCS therapy and offers an evidence-based, non-pharmaceutical alternative to opioid therapy and/or an adjunctive therapy to facilitate opioid dose reduction whilst delivering significant pain relief. Healthcare providers involved in management of chronic non-cancer pain can include reduction or elimination of opioid use as part of treatment plan when contemplating 10 kHz SCS.
Downregulation of Sostdc1 in Testicular Sertoli Cells is Prerequisite for Onset of Robust Spermatogenesis at Puberty
Authors: Pradhan Bhola Shankar , Bhattacharya Indrashis , Sarkar Rajesh , Majumdar Subeer S. 
Published: August 2019

AbstractAn alarming decline in sperm count of men from several countries has become a major concern for the world community. Hormones act on testicular Sertoli cells (Sc) to regulate male fertility by governing the division and differentiation of germ cells (Gc). However, there is a limited knowledge about Sc specific gene(s) regulating the spermatogenic output of the testis. Sclerostin domain-containing 1 protein (Sostdc1) is a dual BMP/Wnt regulator is predominantly expressed in the Sc of infant testes which hardly show any sign of spermatogenesis. In order to investigate the role of Sostdc1 in spermatogenic regulation, we have generated transgenic (Tg) rats which induced persistent expression of Sostdc1 in mature Sc causing reduced sperm counts. Although Sc specific Sostdc1 did not affect the function of either Sc or Leydig cells (Lc) in the adult testis of Tg rat, we observed a selective augmentation of the BMP target genes via activated phospho smad 1/5/8 signaling in Gc leading to apoptosis. Here, for the first time, we have demonstrated that Sostdc1 is a negative regulator of spermatogenesis, and provided substantial evidence that down regulation of Sostdc1 during puberty is critically essential for quantitatively and qualitatively normal spermatogenesis governing male fertility.
Lipophilic statins inhibit Zika virus production in Vero cells
Authors: Españo Erica , Nam Jeong-Hyun , Song Eun-Jung , Song Daesub , Lee Chong-Kil , Kim Jeong-Ki 
Published: August 2019

AbstractZika virus (ZIKV) is a mosquito-borne member of the Flaviviridae family. ZIKV infection has been associated with neurological complications such as microcephaly in newborns and Guillain-Barré syndrome in adults; thus, therapeutic agents are urgently needed. Statins are clinically approved for lowering cholesterol levels to prevent cardiovascular disease but have shown potential as antiviral drugs. In this study, we explored the possibility of utilizing statins as anti-ZIKV drugs. We found that, generally, lipophilic statins (atorvastatin, cerivastatin, fluvastatin, lovastatin, mevastatin, and simvastatin) could reduce ZIKV production in vitro and result in smaller foci of infection. Time-of-drug-addition assay revealed that early treatment with statins is more beneficial than late treatment; however, statins could not completely inhibit the entry stage of ZIKV infection. Furthermore, individual lipophilic statins differed in anti-ZIKV capacity, with fluvastatin being the most efficient at low concentrations. Taken together, this study shows that statins or their derivatives have the potential to be used as anti-ZIKV therapeutic agents.