bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid and reproducible phosphopeptide enrichment by tandem metal oxide affinity chromatography: application to boron deficiency induced phosphoproteomics
Authors: Chen Yanmei , Hoehenwarter Wolfgang 
Published: December 2018

SummaryMass spectrometry has been instrumental in enabling the study of molecular signaling on a cellular scale by way of site‐specific quantification of protein post‐translational modifications, in particular phosphorylation. Here we describe an updated tandem metal oxide affinity chromatography (MOAC) combined phosphoprotein/phosphopeptide enrichment strategy, a scalable phosphoproteomics approach that allows rapid identification of thousands of phosphopeptides in plant materials. We implemented modifications to several steps of the original tandem MOAC procedure to increase the amount of quantified phosphopeptides and hence site‐specific phosphorylation of proteins in a sample beginning with the less amounts of tissue and a substantially smaller amount of extracted protein. We applied this technology to generate time‐resolved maps of boron signaling in Arabidopsis roots. We show that the successive enrichment of phosphoproteins in a first and phosphopeptide extraction in a second step using our optimized procedure strongly enriched the root phosphoproteome. Our results reveal that boron deficiency affects over 20% of the measured root phosphoproteome and that many phosphorylation sites with known biological function, and an even larger number of previously undescribed sites, are modified during the time course of boron deficiency. We identify transcription factors as key regulators of hormone signaling pathways that modulate gene expression in boron deprived plants. Furthermore, our phosphorylation kinetics data demonstrate that mitogen‐activated protein kinase (MAPK) cascades mediate polarized transport of boron in Arabidopsis roots. Taken together, we establish and validate a robust approach for proteome‐wide phosphorylation analysis in plant biology research.
What Will You Do for the Rest of the Day?
Authors: Sadri Amin , Salim Flora D. , Ren Yongli , Shao Wei , Krumm John C. , Mascolo Cecilia 
Published: December 2018

Understanding and predicting human mobility is vital to a large number of applications, ranging from recommendations to safety and urban service planning. In some travel applications, the ability to accurately predict the user's future trajectory is vital for delivering high quality of service. The accurate prediction of detailed trajectories would empower location-based service providers with the ability to deliver more precise recommendations to users. Existing work on human mobility prediction has mainly focused on the prediction of the next location (or the set of locations) visited by the user, rather than on the prediction of the continuous trajectory (sequences of further locations and the corresponding arrival and departure times). Furthermore, existing approaches often return predicted locations as regions with coarse granularity rather than geographical coordinates, which limits the practicality of the prediction.
In this paper, we introduce a novel trajectory prediction problem: given historical data and a user's initial trajectory in the morning, can we predict the user's full trajectory later in the day (e.g. the afternoon trajectory)? The predicted continuous trajectory includes the sequence of future locations, the stay times, and the departure times. We first conduct a comprehensive analysis about the relationship between morning trajectories and the corresponding afternoon trajectories, and found there is a positive correlation between them. Our proposed method combines similarity metrics over the extracted temporal sequences of locations to estimate similar informative segments across user trajectories.
Our evaluation shows results on both labeled and geographical trajectories with a prediction error reduced by 10-35% in comparison to the baselines. This improvement has the potential to enable precise location services, raising usefulness to users to unprecedented levels. We also present empirical evaluations with Markov model and Long Short Term Memory (LSTM), a state-of-the-art Recurrent Neural Network model. Our proposed method is shown to be more effective when smaller number of samples are used and is exponentially more efficient than LSTM.
A Language for Online State Processing of Binary Sensors, Applied to Ambient Assisted Living
Authors: Volanschi Nic , Serpette Bernard , Carteron Adrien , Consel Charles 
Published: December 2018

There is a large variety of binary sensors in use today, and useful context-aware services can be defined using such binary sensors. However, the currently available approaches for programming context-aware services do not conveniently support binary sensors. Indeed, no existing approach simultaneously supports a notion of state, central to binary sensors, offers a complete set of operators to compose states, allows to define reusable abstractions by means of such compositions, and implements efficient online processing of these operators.
This paper proposes a new language for event processing specifically targeted to binary sensors. The central contributions of this language are a native notion of state and semi-causal operators for temporal state composition including: Allen's interval relations generalized for handling multiple intervals, and temporal filters for handling delays. Compared to other approaches such as CEP (complex event processing), our language provides less discontinued information, allows less restricted compositions, and supports reusable abstractions. We implemented an interpreter for our language and applied it to successfully rewrite a full set of real Ambient Assisted Living services. The performance of our prototype interpreter is shown to compete well with a commercial CEP engine when expressing the same services.
Hepcidin Deficiency Protects Against Atherosclerosis
Authors: Malhotra Rajeev , Wunderer Florian , Barnes Hanna J. , Bagchi Aranya , Buswell Mary D. , O’Rourke Caitlin D. , Slocum Charles L. , Ledsky Clara D. , Peneyra Kathryn M. , Sigurslid Haakon , Corman Benjamin , Johansson Kimberly B. , Rhee David K. , Bloch Kenneth D. , Bloch Donald B. 
Published: December 2018

Objective—
Inflammatory stimuli enhance the progression of atherosclerotic disease. Inflammation also increases the expression of hepcidin, a hormonal regulator of iron homeostasis, which decreases intestinal iron absorption, reduces serum iron levels and traps iron within macrophages. The role of macrophage iron in the development of atherosclerosis remains incompletely understood. The objective of this study was to investigate the effects of hepcidin deficiency and decreased macrophage iron on the development of atherosclerosis.

Approach and Results—

Hepcidin- and LDL (low-density lipoprotein) receptor-deficient (
Hamp
−/−
/
Ldlr
−/−
) mice and
Hamp
+/+
/
Ldlr
−/−
control mice were fed a high-fat diet for 21 weeks. Compared with control mice,
Hamp
−/−
/
Ldlr
−/−
mice had decreased aortic macrophage activity and atherosclerosis. Because hepcidin deficiency is associated with both increased serum iron and decreased macrophage iron, the possibility that increased serum iron was responsible for decreased atherosclerosis in
Hamp
−/−
/
Ldlr
−/−
mice was considered.
Hamp
+/+
/
Ldlr
−/−
mice were treated with iron dextran so as to produce a 2-fold increase in serum iron. Increased serum iron did not decrease atherosclerosis in
Hamp
+/+
/
Ldlr
−/−
mice. Aortic macrophages from
Hamp
−/−
/
Ldlr
−/−
mice had less labile free iron and exhibited a reduced proinflammatory (M1) phenotype compared with macrophages from
Hamp
+/+
/
Ldlr
−/−
mice. THP1 human macrophages treated with an iron chelator were used to model hepcidin deficiency in vitro. Treatment with an iron chelator reduced LPS (lipopolysaccharide)-induced M1 phenotypic expression and decreased uptake of oxidized LDL.

Conclusions—
In summary, in a hyperlipidemic mouse model, hepcidin deficiency was associated with decreased macrophage iron, a reduced aortic macrophage inflammatory phenotype and protection from atherosclerosis. The results indicate that decreasing hepcidin activity, with the resulting decrease in macrophage iron, may prove to be a novel strategy for the treatment of atherosclerosis.
The new and the old: littoral tanaidomorph Tanaidacea (Crustacea: Peracarida) from the Andaman Islands, Indian Ocean
Authors: BAMBER ROGER N , CHATTERJEE TAPAS 
Published: December 2018

Recent collections of tanaidaceans from intertidal macroalgae on the shores of the Andaman Islands have revealed both new taxa to science and new material of a species which had previously been inadequately described. The present paper gives a redescription of the leptocheliid Konarus crassicornis, discussing its close similarity to the generotype, K. cheirus, and describes two new species, one of the tanaid genus Zeuxo, showing similarities to Z. (Parazeuxo) kurilensis and representing the first anatanaid to be recorded from the region, and one paratanaid of a new genus, Triparatanais, distinguished from other paratanaids in having only three longer articles in the antennule.
Thirty-Day Outcomes of Fenestrated and Chimney Endovascular Repair and Open Repair of Juxtarenal, Pararenal, and Suprarenal Abdominal Aortic Aneurysms Using National Surgical Quality Initiative Program Database (2012-2016)
Authors: Locham Satinderjit , Dakour-Aridi Hanaa , Bhela Jatminderpal , Nejim Besma , Bhavana Challa Apurva , Malas Mahmoud 
Published: December 2018

Background: Fenestrated endovascular repair (FEVAR) and chimney endovascular repair (ChEVAR) endovascular repair offer a less invasive alternative to open aortic repair (OAR) in managing juxtarenal, pararenal, and suprarenal abdominal aortic aneurysms (AAAs). The aim of this study is to evaluate the 30-day postoperative outcomes following endovascular and open repair of nonruptured AAA involving the renal vessels. Study Design: All patients undergoing endovascular (FEVAR and ChEVAR) and open repair of juxtarenal, pararenal, and suprarenal AAA in National Surgical Quality Improvement Program database from 2012 to 2016 were included. Continuous and categorical covariates were analyzed using medians and χ2/Fisher exact test, respectively. Multivariable logistic regression analyses were performed to evaluate primary (mortality) and secondary (renal and cardiopulmonary failure) outcomes between open versus endovascular approach. Results: A total of 1191 patients underwent AAA repair using open (72%) or endovascular (FEVAR: 14%, ChEVAR: 14%) approach. In univariate analysis, no significant difference in 30-day mortality was seen between the 3 groups (FEVAR: 2.47% vs ChEVAR: 7.32% vs OAR: 6.13%, P = .13). However, 30-day major complications including renal failure (9.36% vs 6.10% vs 1.85%, P = .003) and cardiopulmonary complications (19.77% vs 3.66% vs 4.94%, P < 001) failure were significantly higher in patients undergoing OAR versus ChEVAR versus FEVAR. After adjusting for potential confounders, OAR was associated with 2- to 5-folds increased risk of mortality (odds ratio, OR [95% confidence interval, CI]: 2.14 [1.09-4.21], P = .03), renal (OR [95% CI]: 2.87 [1.48-5.57], P = .002), and cardiopulmonary failure (OR [95% CI]: 4.63 [2.47-8.67], P < .001) compared to any endovascular repair. Conclusion: Using a large national surgical data set, our study found 2- to 5-folds higher mortality and morbidity in patients undergoing open versus endovascular repair of AAA involving the renal vessels. Endovascular repair seems to be a safer approach, especially when managing older patients with AAA.
Memory gaps, lost words and crucial mistakes – Men’s experiences of cognitive difficulties in fibromyalgia
Authors: Sallinen Merja , Marit Mengshoel Anne 
Published: December 2018

Objectives The purpose of the study was to explore the impact of perceived cognitive problems on daily life in men with fibromyalgia. Method The data were collected through life-story interviews of eight men with fibromyalgia. Narrative analysis was conducted to create model narratives that comprise the content and storylines across the data. Results Three model narratives are presented in the findings to describe the scope and severity of the cognitive problems. The participants’ experiences of cognitive problems varied from occasional concentration problems to severe and complex difficulties in memory, problem solving and verbal fluency. The impact of these problems on daily life was substantial and overshadowed the participants’ domestic life, social relations and work performance. In some cases, maintaining a role in working life was perceived as challenging. Discussion These patients are usually examined and treated in primary health care settings and the assessment tools and interventions should be applicable in these settings. More research is needed to develop effective interventions that address not only memory but also individual’s ability to solve complex problems and to work under pressure as well as the level of verbal fluency, which all are relevant issues in terms of work ability in contemporary society.
Imaging in multiple myeloma: How? When?
Authors: Zamagni Elena , Tacchetti Paola , Cavo Michele 
Published: December 2018

AbstractBone disease is the most frequent feature of multiple myeloma (MM) and represents a marker of end-organ damage; it is used to establish the diagnosis and to dictate the immediate need for therapy. For this reason, imaging plays a significant role in the management of MM patients. Although conventional radiography has traditionally been the standard imaging modality, its low sensitivity in detecting osteolytic lesions and inability to evaluate response to therapy has called for the use of more sophisticated techniques, such as whole-body low-dose computed tomography (WBLDCT), whole-body magnetic resonance imaging, and 18F-fluorodeoxyglucose–positron emission tomography/computed tomography (PET/CT). In this review, the advantages, indications of use, and applications of the 3 techniques in the management of patients with MM in different settings will be discussed. The European Myeloma Network and the European Society for Medical Oncology guidelines have recommended WBLDCT as the imaging modality of choice for the initial assessment of MM-related lytic bone lesions. Magnetic resonance imaging is the gold-standard imaging modality for detection of bone marrow involvement, whereas PET/CT provides valuable prognostic data and is the preferred technique for assessment of response to therapy. Standardization of most of the techniques is ongoing.
Physical Activity and Sedentary Behavior among US Hispanic/Latino Youth: The SOL Youth Study
Authors: EVENSON KELLY R. , ARREDONDO ELVA M. , CARNETHON MERCEDES R. , DELAMATER ALAN M. , GALLO LINDA C. , ISASI CARMEN R. , PERREIRA KRISTA M. , FOTI SAMANTHA A. , VAN HORN LINDA , VIDOT DENISE C. , SOTRES-ALVAREZ DANIELA 
Published: December 2018

ABSTRACT

Purpose
Physical activity and sedentary behavior among diverse Hispanic/Latino youth in the United States is not well documented. The aim of this study was to describe physical activity and sedentary behavior among a representative sample of Hispanic/Latino youth from four US communities using accelerometry and self-reported measures.

Methods
From 2012 to 2014, 1466 Hispanic/Latino youth ages 8 to 16 yr, children of participants in the Hispanic Community Health Study/Study of Latinos, enrolled in the SOL youth. Physical activity and sedentary behavior were assessed by interview. After this, youth wore an Actical accelerometer for 1 wk. All statistical analyses accounted for the complex survey design and used sampling weights.

Results
The accelerometer wear time adjusted mean minutes per day was: 604.6, sedentary; 178.9, light; 25.4, moderate; and 10.2, vigorous. Generally, higher levels of moderate and vigorous activity occurred among males, Mexican backgrounds, and youth age 8 to 10 yr compared with older age groups. Higher levels of sedentary behavior occurred among youth age 15 to 16 yr compared with younger age groups. The most common activities (reported, ≥1 per month) were of lower intensity, including listening to music (91.9%), homework (87.0%), riding in car/bus (84.3%), and hanging out with friends (83.4%). Common active pursuits included travel by walking (74.6%), physical education class (71.7%), running (71.4%), and recess (71.3%).

Conclusions
Time, intensity, and type of physical activity and sedentary behavior varied among Hispanic/Latino youth. These findings can inform efforts to increase physical activity and reduce sedentary behavior among US Hispanic/Latino youth.
Life on the fringe: microbial adaptation to growth on carbon monoxide
Authors: Robb Frank T. , Techtmann Stephen M. 
Published: December 2018

Microbial adaptation to extreme conditions takes many forms, including specialized metabolism which may be crucial to survival in adverse conditions. Here, we analyze the diversity and environmental importance of systems allowing microbial carbon monoxide (CO) metabolism. CO is a toxic gas that can poison most organisms because of its tight binding to metalloproteins. Microbial CO uptake was first noted by Kluyver and Schnellen in 1947, and since then many microbes using CO via oxidation have emerged. Many strains use molecular oxygen as the electron acceptor for aerobic oxidation of CO using Mo-containing CO oxidoreductase enzymes named CO dehydrogenase. Anaerobic carboxydotrophs oxidize CO using CooS enzymes that contain Ni/Fe catalytic centers and are unrelated to CO dehydrogenase. Though rare on Earth in free form, CO is an important intermediate compound in anaerobic carbon cycling, as it can be coupled to acetogenesis, methanogenesis, hydrogenogenesis, and metal reduction. Many microbial species—both bacteria and archaea—have been shown to use CO to conserve energy or fix cell carbon or both. Microbial CO formation is also very common. Carboxydotrophs thus glean energy and fix carbon from a “metabolic leftover” that is not consumed by, and is toxic to, most microorganisms. Surprisingly, many species are able to thrive under culture headspaces sometimes exceeding 1 atmosphere of CO. It appears that carboxydotrophs are adapted to provide a metabolic “currency exchange” system in microbial communities in which CO arising either abiotically or biogenically is converted to CO2and H2that feed major metabolic pathways for energy conservation or carbon fixation. Solventogenic CO metabolism has been exploited to construct very large gas fermentation plants converting CO-rich industrial flue emissions into biofuels and chemical feedstocks, creating renewable energy while mitigating global warming. The use of thermostable CO dehydrogenase enzymes to construct sensitive CO gas sensors is also in progress.
What's that rash? Key dermatological presentations for the paramedic
Authors: Kirkby John , Eaton Georgette 
Published: December 2018

Overview This article presents an overview of the additional aspects of history-taking that need to be considered when assessing patients presenting with a rash. This relies on strong underpinning anatomy and physiology knowledge to correlate the skin presentation to what may be happening pathophysiologically within the patient's skin. Four case studies are presented, with leading questions which are then explained. The overall aim of this article is to provide an introduction to key dermatological presentations for the paramedic as a starting point for more thorough reading and exploration on the subject.
Réinsertion des auteurs d’infractions à caractère sexuel : réflexion et échanges avec un responsable d’une communauté d’Emmaüs en région Centre-Val de Loire (France)
Authors: Courtois Robert , Mozas Emmanuel , Bertsch Ingrid 
Published: December 2018

Les personnes accueillies dans une communauté d’Emmaüs représentent une population très hétérogène au sein de laquelle il peut y avoir des auteurs d’infractions à caractère sexuel (AICS). Ces communautés offrent théoriquement à toutes personnes la possibilité de les rejoindre pourvu qu’en échange elles puissent travailler pour faire vivre la collectivité. La compétence n’est pas nécessairement requise, car le travail est adapté aux possibilités de chacun. Cela peut permettre aussi bien à une personne souffrant de troubles psychiques chroniques comme la schizophrénie d’y trouver sa place (si sa maladie est suffisamment stabilisée pour supporter un minimum de contrainte liées à la vie collective), qu’à un AICS en situation post-pénale et en situation d’exclusion sociale.
Les AICS ont besoin d’avoir des lieux de vie qui peuvent les accueillir qui ne sont pas si nombreux, surtout en cas de violences intrafamiliales. Ils vont cumuler des difficultés multiples : faibles ressources financières, faible niveau d’étude et de formation, auxquelles seront associées des difficultés de réinsertion sociale. Celles-ci peuvent représenter autant de facteurs de risque de récidive de l’acte délictueux ou criminel pour lequel ils ont été condamnés ou pour tout autre forme de violence. Mais si l’accueil dans une communauté de vie représente une forme de « protection » par la dimension contenante qu’elle offre, les interactions sociales qu’elle permet, il n’est pas une garantie contre la reprise d’une carrière délinquante ou criminelle. Les communautés d’Emmaüs permettent l’intégration dans une communauté de vie et un retour au sein de la société pour que les personnes accueillies puissent un jour mener à bien ou non leur projet de réinsertion sociale.
Postretrieval Relearning Strengthens Hippocampal Memories via Destabilization and Reconsolidation
Authors: Tay Kai Rong , Flavell Charlotte R. , Cassini Lindsey , Wimber Maria , Lee Jonathan L.C. 
Published: December 2018

Memory reconsolidation is hypothesized to be a mechanism by which memories can be updated with new information. Such updating has previously been shown to weaken memory expression or change the nature of the memory. Here we demonstrate that retrieval-induced memory destabilization also allows that memory to be strengthened by additional learning. We show that for rodent contextual fear memories, this retrieval conditioning effect is observed only when conditioning occurs within a specific temporal window opened by retrieval. Moreover, it necessitates hippocampal protein degradation at the proteasome and engages hippocampal Zif268 protein expression, both of which are established mechanisms of memory destabilization-reconsolidation. We also demonstrate a conceptually analogous pattern of results in human visual paired-associate learning. Retrieval-relearning strengthens memory performance, again only when relearning occurs within the temporal window of memory reconsolidation. These findings link retrieval-mediated learning in humans to the reconsolidation literature, and have potential implications both for the understanding of endogenous memory gains and strategies to boost weakly learned memories.SIGNIFICANCE STATEMENTMemory reconsolidation allows existing memories to be updated with new information. Previous research has demonstrated that reconsolidation can be manipulated pharmacologically and behaviorally to impair problematic memories. In this article, we show that reconsolidation can also be exploited to strengthen memory. This is shown both in rats, in a fear memory setting, and in a human declarative memory setting. For both, the behavioral conditions necessary to observe the memory strengthening match those that are required to trigger memory reconsolidation. There are several behavioral approaches that have previously been shown convincingly to strengthen memory. The present demonstration that reconsolidation can underpin long-lasting memory improvements may both provide an underlying mechanism for such approaches and provide new strategies to boost memories.