bluuebunny/crossref_metadata_2025_split huggingface dataset:
Faecal sample storage without ethanol for up to 24 h followed by freezing performs better than storage with ethanol for shotgun metagenomic microbiome analysis in patients with inflammatory and non-inflammatory intestinal diseases and healthy controls
Authors: Grønbæk Ida Marie Bruun , Mollerup Sarah , Halkjær Sofie Ingdam , Paulsen Sarah Juel , Pinholt Mette , Westh Henrik , Petersen Andreas Munk 
Published: November 2024

Abstract
Objective
The influence of different faecal collection methods on metagenomic analyses remains under discussion, and there is no general agreement on which collection method is preferable for gut microbiome research. We compared faecal samples collected in tubes without preservatives with those containing 10 mL of 96% ethanol for gut microbiome research when the timeframe from defecation to freezing at – 80 °C was up to 24 h. We aimed to compare the collection methods on faeces from participants with inflammatory and non-inflammatory gastrointestinal disorders and healthy controls to investigate the most suitable method when considering data yield, human fraction of sequencing reads, and ease of use. We also examined the faecal sample homogeneity.

Results
Faeces collected in tubes without preservatives resulted in more sequencing reads compared to faeces collected in tubes with 96% ethanol and were also easier to handle. The human fraction of total reads in faeces collected in ethanol from participants with inflammatory bowel disease was higher than all other samples. DNA extraction and sequencing from two different locations in the same faecal sample gave similar results and showed sample homogeneity.
Effects of establishing infection control program with core components of World Health Organization on reducing the risk of residents’ infections and improving staff infection control competency in a nursing home
Authors: Lee Min Hye , Yi Yu Mi , Noh Eun-Young , Park Yeon-Hwan 
Published: November 2024

Abstract
Background
Nursing homes (NHs) are high-risk facilities with limited infection control resources and residents susceptible to infectious diseases. The evidence regarding World Health Organization (WHO) core components in NHs is lacking. This study evaluates the effectiveness of establishing an infection prevention and control (IPC) program with WHO’s core components in an NH.

Methods
The IPC program, encompassing evidence-based guidelines, education and training, surveillance, multimodal strategies, monitoring and feedback, workload and staffing considerations, and the built environment, was implemented in a 130-bed NH for one year. The effects were assessed based on the number of infections among residents, the level of knowledge, and the performance of infection control among staff. The risk of infection was analyzed across three phases: pre-implementation phase, implementation phase (6 and 12 months after intervention initiation), and sustainability phase (3, 6, and 12 months after intervention was finished). Staff data were analyzed before and after the intervention.

Results
Analysis of 18,124 resident-days revealed that during the sustainability phase, the risk of respiratory tract infection was significantly lower than before intervention implementation (odds ratio [OR] 0.51, 95% CI 0.30–0.86, p = 0.012). Moreover, a significant improvement was observed in staff knowledge (p = 0.002) and performance (p < 0.001) after the intervention compared to before.

Conclusions
WHO’s core components may have a potential effect on reducing healthcare-associated infections among residents and enhancing the infection control competency of staff in the NH with limited IPC resources.
Malignant pleural effusion facilitates the establishment and maintenance of tumor organoid biobank with multiple patient-derived lung tumor cell sources
Authors: Wang Lingwei , Yu Yanli , Fang Yanhua , Li Yanjiao , Yu Weiting , Wang Zhe , Lv Jinyan , Wang Ruoyu , Liang Shanshan 
Published: November 2024

AbstractThe Patient-Derived Organoids (PDOs) has demonstrated significant potential in personalized medicine. However, the initial establishment of lung cancer organoids (LCOs), and timely therapeutic recommendations face several challenges. Particularly, the current culture systems have not yet achieved the capability to long-term cultivation of all lung tumor sample sources, including malignant pleural effusion (MPE), which poses significant barriers to the rapid clinical translation of PDOs. Here, we established a LCOs biobank derived from various tumor cell origins and investigated the impact of supplementing culture media with MPE supernatant on organoid formation, culture duration, and drug sensitivity. Our findings indicate that MPE can enhance the successful rate of LCOs by extending the passage number and promoting the initial formation of difficult-to-culture samples, such as those derived from MPE or cell lines that were previously unsuccessful in Airway Organoid (AO) medium. MPE also facilitates the rapid proliferation of LCOs, reducing the culture duration by over 50%. Additionally, LCOs exhibit increased chemoresistance in the presence of MPE, which modifies stem cell distribution and reshapes the internal structure of the organoids. Overall, this study highlights the significance of MPE in facilitating the establishment and maintenance of LCOs, and its potential for translational applications in lung cancer research and personalized.
Hepatic methotrexate-associated lymphoproliferative disease: a case report and literature review
Authors: Sakamoto Shinya , Tabuchi Motoyasu , Yoshimatsu Rika , Matsumoto Manabu , Iwata Jun , Okabayashi Takehiro 
Published: November 2024

Abstract
Background
Methotrexate-associated lymphoproliferative disease (MTX–LPD) is a rare and life-threatening complication of MTX administration. MTX–LPD features more extranodal lesions than malignant lymphoma; however, the liver is an extremely rare organ that develops LPD. Herein, we present a case of hepatic MTX–LPD treated with surgical resection. We also reviewed the literature on hepatic MTX–LPD.

Case presentation
A 66-year-old man with a history of rheumatoid arthritis (RA) was admitted to our department for the treatment of hepatic solitary liver tumor. The patient had been receiving MTX (14 mg/week) for RA for 6 years. MTX was withdrawn and salazosulfapyridine was prescribed 3 weeks prior to admission because of mediastinal MTX–LPD. Abdominal contrast-enhanced computed tomography showed a slightly ring-like enhanced hypovascularized mass (80 mm) in the lateral section of the liver. Carbohydrate antigen 19-9 (78.1 U/mL) level was elevated. No evidence was observed on esophagogastroduodenoscopy or colonoscopy. The tumor was suspected to be an intrahepatic cholangiocarcinoma. The patient underwent hepatic lateral sectionectomy and lymphadenectomy. Pathological examination revealed that the hepatic mass was coagulative necrosis of the CD20-positive B-cell lymphocytes. These histological findings were similar to those of rapid necrotic lymphoma. MTX–LPD is known to spontaneously regress after withdrawing MTX, and the patient was diagnosed with hepatic MTX–LPD.

Conclusions
MTX–LPD can occur in the liver. Clinician should suspect hepatic MTX–LPD when a liver mass is detected in patient who had been treating with MTX for RA.
Quantification of breast biopsy clip marker artifact on routine breast MRI sequences: a phantom study
Authors: Kremser Christian , Gruber Leonhard , Dietzel Matthias , Amort Birgit , Santner Wolfram , Daniaux Martin 
Published: November 2024

Abstract
Background
To investigate the artifact sizes of four common breast clip-markers on a standard breast magnetic resonance imaging (MRI) protocol in an in vitro phantom model.

Methods
Using 1.5-T and 3-T whole-body scanners with an 18-channel breast coil, artifact dimensions of four breast biopsy markers in an agarose-gel phantom were measured by two readers on images obtained with the following sequences: T2-weighted fast spin-echo short inversion time fat-suppressed inversion-recovery with magnitude reconstruction (T2-TIRM); T1-weighted spoiled gradient-echo with fat suppression (T1_FL3D), routinely used for dynamic contrast-enhanced imaging; diffusion-weighted imaging (DWI), including a readout segmented echo-planar imaging (RESOLVE-DWI) and echo-planar imaging sequence (EPI-DWI). After outlining the artifacts by freehand regions of interest, sagittal and lateral diameters in axial images were measured.

Results
Interreader agreement for artifact size quantification was high, depending on the sequence (80.4–94.8%). Overall, the size, shape, and appearance of artifacts depended on clip type and MRI sequence. The artifact size ranged from 5.7 × 8.5 mm2 to 13.4 × 17.7 mm2 at 1.5 T and from 6.6 × 8.2 mm2 to 17.7 × 20.7 mm2 at 3 T. Clip artifacts were largest on EPI-DWI and RESOLVE-DWI (p ≤ 0.016). In three out of four clips, T2-TIRM showed the smallest artifact (p ≤ 0.002), while in one clip the artifact was smallest on T1_FL3D (p = 0.026). With the exception of one clip in the RESOLVE sequence, all clips showed a decrease in the artifact area from DWI to ADC images (p ≤ 0.037).

Conclusion
Breast clip-marker MRI artifact appearances depend on clip type, field strength, and sequence and may reach a significant size, potentially obscuring smaller lesions and hindering accurate assessment of breast tumors.

Relevance statement
Considerable variations in artifact size and characteristics across different breast clips, MRI sequences, and field strengths exist. Awareness of these artifacts and their characteristics is essential to ensure accurate interpretation of scans and appropriate treatment planning.

Key Points

Awareness of breast clip artifacts is essential for accurate interpretation of MRI.

The appearance of artifacts depends on breast clip type, field strength, and sequence.

Clip-related artifacts might hinder the visibility of small lesions.

Graphical Abstract
Relationships between cognition, functioning, and quality of life of euthymic patients with bipolar disorder: Structural equation modeling with the FACE-BD cohort
Authors: Roux P , Frileux S , Vidal N , Aubin V , Belzeaux R , Courtet P , Dubertret C , Etain B , Haffen E , Leboyer M , Lefrere A , Llorca PM , M’Bailara K , Marlinge E , Olié E , Polosan M , Schwan R ,   The FACE-BD (FondaMental Academic Centers of Expertise for Bipolar Disorders) group, Brunet-Gouet E , Passerieux C 
Published: November 2024

Abstract

Background
Quality of life is decreased in bipolar disorders (BD) and contributes to poor prognosis. However, little is known about the causal pathways that may affect it. This study aimed to explore health-related QoL (HRQoL) in BD and investigate its relationship with cognition and psychosocial functioning.

Methods
This multicenter cross-sectional study used a neuropsychological battery to assess five cognition domains. Functioning was evaluated using global and domain-based tools, and health-related HRQoL was assessed using the EQ-5D-3L. Structural equation modeling was used to test whether the association between cognition and HRQoL would be mediated by functioning in BD while controlling for covariates such as residual depression, anxiety, antipsychotic medication, and psychotic features.

Results
We included 1 190 adults with euthymic BD. The model provided a good fit for the data. In this model, the direct effect of cognition on HRQoL was not significant (β = − 0.03, z = −0.78, p = 0.433). The total effect of cognition on HRQoL was weak, albeit significant (β = 0.05, z = 3.6, p < 0.001), thus suggesting that cognition affected HRQoL only indirectly through functioning. Anxiety was associated with decreased functioning (β = −0.27, z = −7.4, p < 0.001) and QoL (β = −0.39, z = −11.8, p < 0.001).

Conclusions
These findings suggest that improving cognition may not directly lead to a higher HRQoL. Cognitive remediation is expected to improve HRQoL only through functioning enhancement. They also reveal the potential importance of functional remediation and reduction of comorbid anxiety symptoms in improving HRQoL in BD.
Does Experience of Vaccination Improve Vaccine Confidence and Trust? Policy Feedback Effects of Mass COVID-19 Vaccination in the United States
Authors: Choi Yongjin , Fox Ashley 
Published: November 2024

Abstract
Context: Policy feedback research has demonstrated that a highly tangible policy that shapes public attitudes through direct and day-to-day experiences often reshapes public opinion, with the effect of generating supportive or skeptical constituencies that determine the sustainability of future programs. This paper examines the impact of mass vaccination efforts on attitudes towards vaccines in a context of high vaccine hesitancy in the U.S.
Methods: We analyzed 73,092 survey responses from 9,229 participants in the longitudinal data from the Understanding America Study project, covering December 2020 to July 2023. Using two-way fixed-effects ordinary least squares regression and ordinal logistic regression, we estimated the changes in attitudes towards vaccines, including trust in vaccine manufacturing and approval processes, following COVID-19 vaccinations.
Findings: COVID-19 vaccination was associated with improved perceptions of vaccine effectiveness and social benefits and reduced mistrust in vaccine-related processes. However, it did not significantly alleviate concerns regarding vaccine side effects and illness. The strongest effects were observed among respondents initially hesitant but who eventually vaccinated.
Conclusions: The experience of COVID-19 vaccination generally improved attitudes and confidence in COVID-19 vaccines among the U.S. public, particularly among vaccine-hesitant people. These effects could have positive impacts on future immunization programs by mitigating vaccine hesitancy.
VALUE ORIENTATIONS OF TEENAGERS WITH DIFFERENT LEVELS OF EMOTIONAL INTELLIGENCE
Authors: Anisimova Yulia N. , Khusnutdinova Rezida R. , Kameneva Galina N. 
Published: November 2024

Background. The formation of values in adolescence is one of the priority tasks of school education and upbringing, since it is values that determine a person's relationship with society. In the course of communication and interaction with the surrounding world, adolescents form personal characteristics, including emotional intelligence, adolescents' perception of values and meanings.
Purpose. To study the features of value orientations in adolescents with different levels of emotional intelligence.
Materials and methods. In the course of studying the value orientations of adolescents with different levels of emotional intelligence, an empirical study was conducted with the participation of 50 adolescents aged 14-15 years of Naberezhnye Chelny city schools.
Diagnostic tools of the study included the use of the following methods: ‘Diagnostics of value orientations of adolescents’ (V.F. Sopov L.V. Karpushina), “Value orientations” (M. Rokich), questionnaire “Emotional intelligence” (D.V. Lyusin). Mathematical processing of the results was carried out by Student's t-criterion method.
Results. Adolescent children learn to understand their own emotions, but still have difficulty recognising the emotional states of other people, more often by external expression, while showing little sensitivity to the mental state of others. They have poor control over their emotions and are unable to manage the emotions of their peers and are unable to manage external expressive manifestations of their emotions and restrain facial expressions and gestures. Teenagers display the values ‘cognition’, ‘I-value’, ‘other-value’, socially useful activity is not so significant for teenagers, and they do not consider responsibility a valuable quality.
In the ‘terminal values’ category, it is more important for adolescents to have loyal friends, health, freedom and material well-being. Adolescents are least interested in happiness in family life, happiness of others and social recognition. In the ‘instrumental values’ category, it is more important for adolescents to enjoy life, to be useful in a common cause, and to be well-mannered and educated. Adolescents pay the least attention to responsibility, high demands, and broad-mindedness.
FROM CRITICAL THINKING TO CRITICAL TRUST: THE CASE FOR ONLINE LEARNING
Authors: Golubinskaya Anastasiia V. , Viakhireva Valeriia V. 
Published: November 2024

Background. On one hand, education is a specific form of social interaction where participants’ trust is a prerequisite rather than an outcome. On the other hand, the contemporary informational environment, in which new educational practices emerge, is replete with unverified and false information that complicates trust. This creates a paradox: online education simultaneously demands both swift trust and epistemic vigilance. Swift trust and epistemic vigilance are understood as two regimes of critical thinking, and the factors influencing the switch between these regimes are the subject of this study.
Purpose. To identify the factors that trigger different regimes of critical thinking in online learning.
Materials and methods. The theoretical part of the study is based on the synthesis of epistemic trust concept, theory of swift trust and social-epistemological approach to critical thinking. Based on this, the article presents a pilot survey of students of massive open online courses of Lobachevsky University online learning platform (N=83).
Results. The main finding is that the key factor in switching between swift trust and epistemic vigilance is the previous negative online learning experiences. having such experience is consolidated and sharply reduces expectations from any subsequent online learning. The absence of negative experiences, on the contrary, increases swift trust and reduces epistemic vigilance. The main mechanisms of switching between regimes of critical thinking in online education are presented as epistemic delegation, appeal to statistics, logical-grammatical representation, and visual representation.
Sponsorship information. The work was funded with a grant from the Russian Science Foundation (project № 24-28-00809 “Critical thinking studies: fundamental research on critical thinking as an interdisciplinary problem”)
Advanced therapeutic strategies in patellar ligament tendinopathy: from etiology to clinical practice. A literature review.
Authors: Stachowiak Julia , Sosin Julia , Pilarz Anna , Zwierzchowska Maria , Sojka Aleksandra , Salamon Dariusz , Domagała Wojciech 
Published: November 2024

Introduction:
Tendinopathy of the patellar ligament, often referred to as “jumper's knee,” is a significant health problem for athletes subjected to repeated knee strains. The condition, resulting from microtrauma and degeneration of collagen fibers, leads to chronic pain and impaired function of the patellar ligament.

Aim of the study:
The aim of this study was to present current knowledge about new techniques to prevent and treat patellar tendinopathy. Analyzing current informations, understanding risk factors, and recognizing pathophysiology of patellar tendinopathy can prevent many sportsmen to withdraw from competitions.

Materials and methods:
A systematic review of scientific and medical literature from the PubMed and Google Scholar databases was conducted.
Results:
A review of the literature has identified key risk factors for patellar ligament tendinopathy. An analysis of available studies indicates that various therapeutic interventions, including shockwave therapy, eccentric exercise programs can provide pain relief and improve patellar ligament function. However, the effectiveness of these methods may vary depending on individual patient characteristics.

Conclusions:
Recognition of risk factors, early education and prevention, and prompt introduction of treatment at an early enough stage of the disease allow for the best treatment results and rapid return of patients to sports activities before the onset of pain, and significantly improve patients' quality of life. These findings underscore the comprehensive nature of the management of patellar ligament tendinopathy, including both therapeutic and preventive interventions.

Keywords: patellar tendinopathy, orthopeadics, sport medicine, knee
Research on Teaching Idea Innovation of University Physical Education Teachers
Authors: Huang Jin , Wang Shaojie , Zhu Jun , Cai Jiao , Jiang Bo 
Published: November 2024

This study takes the teaching reform concept of university PE teachers in the new era as the research object, and uses the literature method, logical analysis method and inductive deduction method to deeply analyze the development opportunity and realistic bottleneck of the teaching innovation concept of university PE teachers. The research shows that the teaching innovation concept of university PE teachers faces new challenges, and the generalization of education consciousness and the motivation of concept innovation of university PE teachers are insufficient. "Cultivating morality and cultivating people" as the basic task of education, the author puts forward the concept of "moral education first" in the innovation of teaching concept of physical education teachers in universities. Construct the evaluation system of "cultivating virtue" and "cultivating body"; To construct a new model of post-service education for physical education teachers in universities, and establish a new standard for the evaluation of professional titles of physical education teachers in universities; The new model of cultivating multi-disciplinary high quality sports talents and the concept of PE teachers' education should be integrated with aesthetic education. The aim is to provide theoretical reference for the timely updating of the teaching concept of university PE teachers under the background of the new era and optimizing the classified development of university PE teachers.
Multimodal Discourse Interpretation of the Paris Olympics Mascot
Authors: Xu Longhua , Wang Lei , Luo Yanni 
Published: November 2024

Background: This study aims to analyze the design intention of the mascot of the Paris Olympics from a multimodal perspective. As the host of the 2024 Olympic Games, Paris' mascot carries rich meanings. With globalization and technological advancement, information spreads multimodally, and multimodal discourse analysis is important to understand how the mascot interacts with different audiences, conveys meanings as well as bridges cultural differences, and spreads the Olympic spirit and cultural heritage of the host country.
Methods: Taking Kress and Van Leeuwen's visual grammar as the theoretical basis, based on multimodal discourse analysis under the perspective of social semiotics, and combining with the analysis indexes in the multimodal image analysis software, the mascots of the Paris Olympic Games are visualized and interpreted to explore their image characteristics, design concepts and social significance.
Results: the mascot is analyzed from three aspects: reproduction meaning, composition meaning and interaction meaning. In terms of reproduction, the mascot is related to the Olympic Games and French culture; in terms of composition, the emblem and other elements are the core information carrier, and the color and other elements play an important role; in terms of interaction, the mascot has a variety of interactions with the viewers and so on.
Conclusion: Olympic mascots are important components of Olympic culture. The multimodal discourse analysis comprehensively and accurately interpreted the connotation of the mascot of the Paris Olympic Games, reflecting the information of the organizing country and integrating it with the Olympic movement, reflecting the spirit of sports. The study has deficiencies, and the mascots can be further explored in the real context.