bluuebunny/crossref_metadata_2025_split huggingface dataset:
GluR2 endocytosis-dependent protein degradation in the amygdala mediates memory updating
Authors: Ferrara Nicole C. , Jarome Timothy J. , Cullen Patrick K. , Orsi Sabrina A. , Kwapis Janine L. , Trask Sydney , Pullins Shane E. , Helmstetter Fred J. 
Published: March 2019

AbstractAssociations learned during Pavlovian fear conditioning are rapidly acquired and long lasting, providing an ideal model for studying long-term memory formation, storage, and retrieval. During retrieval, these memories can “destabilize” and become labile, allowing a transient “reconsolidation” window during which the memory can be updated, suggesting that reconsolidation could be an attractive target for the modification of memories related to past traumatic experiences. This memory destabilization process is regulated by protein degradation and GluR2-endocytosis in the amygdala. However, it is currently unknown if retrieval-dependent GluR2-endocytosis in the amygdala is critical for incorporation of new information into the memory trace. We examined whether the addition of new information during memory retrieval required GluR2-endocytosis to modify the original memory. The presentation of two foot shocks of weaker intensity during retrieval resulted in GluR2 endocytosis-dependent increase in fear responding on a later test, suggesting modification of the original memory. This increase in fear expression was associated with increased protein degradation and zif268 expression in the same population of cells in the amygdala, indicating increased destabilization processes and cellular activity, and both were lost following blockade of GluR2-endocytosis. These data suggest that the endocytosis of GluR2-containing AMPA receptors in the amygdala regulates retrieval-induced strengthening of memories for traumatic events by modulating cellular destabilization and activity.
NeuroPIpred: a tool to predict, design and scan insect neuropeptides
Authors: Agrawal Piyush , Kumar Sumit , Singh Archana , Raghava Gajendra P. S. , Singh Indrakant K. 
Published: March 2019

AbstractInsect neuropeptides and their associated receptors have been one of the potential targets for the pest control. The present study describes in silico models developed using natural and modified insect neuropeptides for predicting and designing new neuropeptides. Amino acid composition analysis revealed the preference of residues C, D, E, F, G, N, S, and Y in insect neuropeptides The positional residue preference analysis show that in natural neuropeptides residues like A, N, F, D, P, S, and I are preferred at N terminus and residues like L, R, P, F, N, and G are preferred at C terminus. Prediction models were developed using input features like amino acid and dipeptide composition, binary profiles and implementing different machine learning techniques. Dipeptide composition based SVM model performed best among all the models. In case of NeuroPIpred_DS1, model achieved an accuracy of 86.50% accuracy and 0.73 MCC on training dataset and 83.71% accuracy and 0.67 MCC on validation dataset whereas in case of NeuroPIpred_DS2, model achieved 97.47% accuracy and 0.95 MCC on training dataset and 97.93% accuracy and 0.96 MCC on validation dataset. In order to assist researchers, we created standalone and user friendly web server NeuroPIpred, available at (https://webs.iiitd.edu.in/raghava/neuropipred.)
Possibility of a new order parameter driven by multipolar moment and Fermi surface evolution in CeGe
Authors: Singh Karan , Mukherjee K. 
Published: March 2019

AbstractPolycrystalline CeGe is investigated by means of DC and AC susceptibility, non-linear DC susceptibility, electrical transport and heat capacity measurements in the low temperature regime. This compound shows two peaks at low magnetic field around TI ~ 10.7 and TII ~ 7.3 K due to antiferromagnetic ordering and subsequent spin rearrangement respectively. Investigation of non-linear DC susceptibility reveals a presence of higher order magnetization which results in the development of a new order parameter around TI. This leads to a lowering of symmetry of the magnetic state. The order parameter increases with decreasing temperature and stabilizes around TII. Consequently, the symmetry of the magnetic state is preserved below this transition. Heat capacity and resistivity results indicate the presence of a gap opening around TI on portion of Fermi surface, due to evolution of the Fermi surface. Magnetoresistance behavior and violation of Kohler’s rule suggest that the evolution of Fermi surface changes the symmetry of magnetic state. The observation of new order parameter (which is of second order) is also confirmed from the Landau free energy theory.
A murine model to study vasoreactivity and intravascular flow in lung isograft microvessels
Authors: Regelin Nora , Heyder Susanne , Laschke Matthias W. , Hadizamani Yalda , Borgmann Michèle , Moehrlen Ueli , Schramm René , Bals Robert , Menger Michael D. , Hamacher Jürg 
Published: March 2019

AbstractIntravital microscopy of orthotopic lung tissue is technically demanding, especially for repeated investigations. Therefore, we have established a novel approach, which allows non-invasive repetitive in vivo microscopy of ectopic lung tissue in dorsal skinfold chambers. Syngeneic subpleural peripheral lung tissue and autologous endometrium (control) were transplanted onto the striated muscle within dorsal skinfold chambers of C57BL/6 mice. Grafts were analysed by intravital fluorescence microscopy over 14 days. Angiogenesis occurred in the grafts on day 3, as indicated by sinusoidal microvessels on the grafts’ edges with very slow blood flow, perifocal oedema, and haemorrhage. By day 10, lung transplants were completely revascularized, exhibited a dense network of microvessels with irregular diameters, chaotic angioarchitecture, and high blood flow. Compared to lung tissue, endometrial grafts contained a structured, glomerulus-like vessel architecture with lower blood flow. Despite missing ventilation, hypoxic vasoconstriction of the lung tissue arterioles occurred. In contrast, endometrium tissue arterioles dilated during hypoxia and constricted in hyperoxia. This demonstrates that ectopic lung grafts keep their ability for organ-specific hypoxic vasoconstriction. These findings indicate that our approach is suitable for repetitive in vivo pulmonary microcirculation analyses. The high blood flow and hypoxia-induced vasoconstriction in lung grafts suggest a physiological intrinsic vasoregulation independent of the recipient tissue.
Exponential consensus ranking improves the outcome in docking and receptor ensemble docking
Authors: Palacio-Rodríguez Karen , Lans Isaias , Cavasotto Claudio N. , Cossio Pilar 
Published: March 2019

AbstractConsensus-scoring methods are commonly used with molecular docking in virtual screening campaigns to filter potential ligands for a protein target. Traditional consensus methods combine results from different docking programs by averaging the score or rank of each molecule obtained from individual programs. Unfortunately, these methods fail if one of the docking programs has poor performance, which is likely to occur due to training-set dependencies and scoring-function parameterization. In this work, we introduce a novel consensus method that overcomes these limitations. We combine the results from individual docking programs using a sum of exponential distributions as a function of the molecule rank for each program. We test the method over several benchmark systems using individual and ensembles of target structures from diverse protein families with challenging decoy/ligand datasets. The results demonstrate that the novel method outperforms the best traditional consensus strategies over a wide range of systems. Moreover, because the novel method is based on the rank rather than the score, it is independent of the score units, scales and offsets, which can hinder the combination of results from different structures or programs. Our method is simple and robust, providing a theoretical basis not only for molecular docking but also for any consensus strategy in general.
Characteristics and homogeneity of N6-methylation in human genomes
Authors: Pacini Clare E. , Bradshaw Charles R. , Garrett Nigel J. , Koziol Magdalena J. 
Published: March 2019

AbstractA novel DNA modification, N-6 methylated deoxyadenosine (m6dA), has recently been discovered in eukaryotic genomes. Despite its low abundance in eukaryotes, m6dA is implicated in human diseases such as cancer. It is therefore important to precisely identify and characterize m6dA in the human genome. Here, we identify m6dA sites at nucleotide level, in different human cells, genome wide. We compare m6dA features between distinct human cells and identify m6dA characteristics in human genomes. Our data demonstrates for the first time that despite low m6dA abundance, the m6dA mark does often occur consistently at the same genomic location within a given human cell type, demonstrating m6dA homogeneity. We further show, for the first time, higher levels of m6dA homogeneity within one chromosome. Most m6dA are found on a single chromosome from a diploid sample, suggesting inheritance. Our transcriptome analysis not only indicates that human genes with m6dA are associated with higher RNA transcript levels but identifies allele-specific gene transcripts showing haplotype-specific m6dA methylation, which are implicated in different biological functions. Our analyses demonstrate the precision and consistency by which the m6dA mark occurs within the human genome, suggesting that m6dA marks are precisely inherited in humans.
Pancreatic stone protein/regenerating protein is a potential biomarker for endoplasmic reticulum stress in beta cells
Authors: Stone Stephen , Abreu Damien , Mahadevan Jana , Asada Rie , Kries Kelly , Graf Rolf , Marshall Bess A. , Hershey Tamara , Urano Fumihiko 
Published: March 2019

AbstractEndoplasmic reticulum (ER) stress in beta cells is an important pathogenic component of both type 1 and type 2 diabetes mellitus, as well as genetic forms of diabetes, especially Wolfram syndrome. However, there are currently no convenient ways to assess ER stress in beta cells, raising the need for circulating ER stress markers indicative of beta cell health. Here we show that pancreatic stone protein/regenerating protein (PSP/reg) is a potential biomarker for ER stressed beta cells. PSP/reg levels are elevated in cell culture and mouse models of Wolfram syndrome, a prototype of ER stress-induced diabetes. Moreover, PSP/reg expression is induced by the canonical chemical inducers of ER stress, tunicamycin and thapsigargin. Circulating PSP/reg levels are also increased in some patients with Wolfram syndrome. Our results therefore reveal PSP/reg as a potential biomarker for beta cells under chronic ER stress, as is the case in Wolfram syndrome.
Plasmonic enhanced Cu2O-Au-BFO photocathodes for solar hydrogen production
Authors: Cheng Xiaorong , Gu Shoulin , Centeno Anthony , Dawson Graham 
Published: March 2019

AbstractA novel Cu2O-Au-BFO heterostructure photocathode was constructed which significantly improved the efficiency of photo-generated carrier transfer for solar hydrogen production. A BiFeO3 (BFO) ferroelectric film was synthesized on top of a Cu2O layer by a sputtering process. The BFO layer acted to protect the Cu2O layer from photochemical corrosion, increasing photoelectrochemical (PEC) stability. The p–n heterojunction between Cu2O and BFO layers enhanced the PEC properties by suppressing charge recombination and improved interfacial charge transfer efficiency. When Cu2O and BFO are interfaced by Au Nanoparticles (NPs) the PEC performance was further enhanced, due to hot-electron transfer at the plasmonic resonance. After positive poling, the depolarization field across the whole volume of BFO film drove electrons into the electrolyte solution, inducing a significant anodic shift, Vop of 1.01 V vs. RHE, together with a significantly enhanced photocurrent density of −91 μA/cm2 at 0 V vs. RHE under 100 mW/cm2 illumination. The mechanism was investigated through experimental and theoretivcal calculations.
Reconstruction of Dynamic and Reversible Color Change using Reflectin Protein
Authors: Cai Tiantian , Han Kui , Yang Peilin , Zhu Zhou , Jiang Mengcheng , Huang Yanyi , Xie Can 
Published: March 2019

AbstractCephalopods have remarkable ability to change their body color across a wide range of wavelengths, yet the structural basis remains largely unknown. Reflectin, a protein family assumed to be responsible for structural color in cephalopods, has unique features of higher-order assembly that are tightly regulated by aromatic molecules. Here, we reconstructed the dynamic and reversible color change using purified reflectin protein and demonstrated how the conformational change and the status of assembly led to the change in optical properties. In addition, optical spectral and structural analyses indicated that the “cephalopod-blue” primarily resulted from wavelength-dependent light scattering rather than reflection. Our results suggest a possible role of reflectin in color dynamics. The in vitro reconstruction system we present here may serve as an initial step for designing bio-inspired optical materials based on reflectin protein.
Macro- and Microscopic Analyses of Anatomical Structures of Chinese Gallnuts and Their Functional Adaptation
Authors: Lu Qin , Chen Hang , Wang Chao , Yang Zi-xiang , Lü Pin , Chen Ming-shun , Chen Xiao-ming 
Published: March 2019

AbstractThe galls induced by Schlechtendaia chinensis, Schlechtendaia peitan and Nurudea shiraii on Rhus chinensis and gall induced by Kaburagia rhusicola rhusicola on Rhus potaninii Maxim. are the largest plant galls and have great economic and medical values. We examined the structures of galls and their functional adaptation using various macro- and microscopic techniques. The highly adapted structures include a stalk at the base that is specialized for mechanical support and transport of nutrients for aphids, and a network of vascular bundles which accompanying schizogenous ducts arranged in a way to best support aphid feeding and population growth. There are many circular and semicircular xylems traces in an ensiform gall in cross sectional views, which would provide more nutrition and occupy less space. We infer the evolution trail was flower-like gall, horned gall, circular gall and ensiform gall. And the possible evolutionary trend of the gall was bigger chamber, more stable mechanical supporting, easier for exchanging substance and transporting nutrients.
Shade and nutrient-mediated phenotypic plasticity in the miracle plant Synsepalum dulcificum (Schumach. & Thonn.) Daniell
Authors: Tchokponhoué Dèdéou A. , N’Danikou Sognigbé , Houéto Jacob S. , Achigan-Dako Enoch G. 
Published: March 2019

AbstractPhenotypic plasticity as a change of genotype expression in response to environmental heterogeneity varies in magnitude among crop species and can induce a shift in a plant’s phenology. In Synsepalum dulcificum, a West African orphan fruit tree, such phenological plasticity is not well understood. Here, we hypothesize that light stimulation and changes in organic nutrient availability would induce an accelerated transition in S. dulcificum from its juvenile to its reproductive phase. We grew 14-month-old seedlings of S. dulcificum under a range of nutrient regimes, both in shade and in full sunlight, and measured their survival, vegetative growth, biomass allocation, and transition to reproductive maturity. The results reveal that S. dulcificum responds favourably to both shading and nutrient application, with the shading exhibiting a stronger influence on the measured variables. The species’ morphological plasticity, particularly in terms of plant height and stem diameter, was found to exceed both its fitness and allocational plasticities. Under the conditions examined, we observed an accelerated transition to fruiting, at an age of only 24 months. The observed plasticity suggests S. dulcificum to be an intermediate shade-tolerant species. This finding expands our knowledge on the appropriate environmental conditions for the breeding and cultivation of this species.
An integrated whole genome analysis of Mycobacterium tuberculosis reveals insights into relationship between its genome, transcriptome and methylome
Authors: Gomez-Gonzalez Paula J. , Andreu Nuria , Phelan Jody E. , de Sessions Paola Florez , Glynn Judith R. , Crampin Amelia C. , Campino Susana , Butcher Philip D. , Hibberd Martin L. , Clark Taane G. 
Published: March 2019

AbstractHuman tuberculosis disease (TB), caused by Mycobacterium tuberculosis (Mtb), is a complex disease, with a spectrum of outcomes. Genomic, transcriptomic and methylation studies have revealed differences between Mtb lineages, likely to impact on transmission, virulence and drug resistance. However, so far no studies have integrated sequence-based genomic, transcriptomic and methylation characterisation across a common set of samples, which is critical to understand how DNA sequence and methylation affect RNA expression and, ultimately, Mtb pathogenesis. Here we perform such an integrated analysis across 22 M. tuberculosis clinical isolates, representing ancient (lineage 1) and modern (lineages 2 and 4) strains. The results confirm the presence of lineage-specific differential gene expression, linked to specific SNP-based expression quantitative trait loci: with 10 eQTLs involving SNPs in promoter regions or transcriptional start sites; and 12 involving potential functional impairment of transcriptional regulators. Methylation status was also found to have a role in transcription, with evidence of differential expression in 50 genes across lineage 4 samples. Lack of methylation was associated with three novel variants in mamA, likely to cause loss of function of this enzyme. Overall, our work shows the relationship of DNA sequence and methylation to RNA expression, and differences between ancient and modern lineages. Further studies are needed to verify the functional consequences of the identified mechanisms of gene expression regulation.
Equivalent circuit method for circulating current loss of transposed strands in multi‐turn coil of a canned induction motor
Authors: Wang Dongmei , Liang Yanping , Bian Xu , Gao Lianlian , Wang Chenguang , Qiao Xue 
Published: March 2019

For the particularity of the transposition method in large induction motor, the equivalent circuit method for the circulating current losses of the transposed strands in stator winding with multi‐turn coil is put forward. According to the unified theory of the electromagnetic field‐circuit and combining with the distribution of various leakage magnetic fields of stator winding, the general circuit network model of the stator winding is proposed, which can be applicable to different transposition angles with the consideration of the accurate distribution of leakage magnetic fields. With the basic idea of discrete points and the linearisation, the method for the leakage reactance of the transposed strands is given with an application in a 5.5 MW nuclear power pump canned induction motor, which uses the strand slot leakage reactance and the end leakage reactance to simulate the slot leakage magnetic field and the end leakage magnetic field. Through experiment, the equivalent circuit method for the circulating current losses of the transposed strands in multi‐turn coil is verified, which will provide a reference for the winding design and the winding loss calculation of the large induction motor.
Balintgruppenleitung – Altes und Neues
Authors: Neumeier Rainald 
Published: March 2019

ZusammenfassungDer Teilnehmerkreis von Balint-Gruppen und die Rahmenbedingungen ärztlicher Arbeit zeigen teilweise erhebliche Veränderungen. Das macht neben Vertrautem auch neue Perspektiven zur Fallarbeit und neue Umgangsweisen mit dem Widerstand in Gruppen erforderlich. Dies beinhaltet auch die Beachtung institutioneller Einflüsse. Die vorliegende Arbeit setzt sich mit verschiedenen Aspekten der Gruppenleitung in modernen Balint-Gruppen sowie den daraus folgenden Implikationen für die Ausbildung von Balint-Gruppenleitern auseinander.
Schöne neue Pflegewelt
Authors: Regitz Christine , Krupka Daniel 
Published: March 2019

ZusammenfassungEine digitalisierte Pflege kann die Arbeitsbedingungen optimieren und den Beruf
auch für neue Zielgruppen öffnen. Dafür braucht es eine durchdachte
Weiterbildung, die Führungskräften und Pflegenden einschlägiges IT-Know-how
vermittelt.
Im Werben um Pflegekräfte
Authors: Steinbrück Jana 
Published: March 2019

ZusammenfassungAlgorithmen, die Kandidaten selektieren? Chat-Bots, die die
Bewerber-Kommunikation übernehmen? Recruiting 4.0 – dies ist für Krankenhäuser
eine weit entfernte Welt. Trotzdem müssen sich die handelnden Akteure mit der
Digitalisierung, veränderten Bewerbererwartungen, E-Recruiting und
online-Jobbörsen auseinandersetzen.
Preoperative MRI Evaluation of Relationship between Trigeminal Nerve and Superior Petrosal Vein: Its Role in Treating Trigeminal Neuralgia
Authors: Xiong Nan-Xiang , Zhou Xiao , Yang Bo , Wang Lei , Fu Peng , Yu Hua , Wang Qiangping , Abdelmaksoud Ahmed , Yuan Ye , Liu Weichao , Huang Yizhi , Budrytė Kamilė , Huang Tao , Zheng Xiaoliang 
Published: March 2019

Objective To assess the value of high-resolution magnetic resonance imaging (MRI) and three-dimensional (3D) reconstruction of the trigeminal nerve and the superior petrosal vein (SPV) in visualizing their anatomical relationship in patients with trigeminal neuralgia (TN).
Patients and Methods This study included 97 patients with primary TN who underwent preoperative 3D constructive interference in steady state (CISS) MRI. Image analysis was performed by an independent observer blinded to the operative findings and then compared with surgical data. The 3D reconstruction was assessed dynamically using MIMICS software (Materialise Inc., Leuven, Belgium).
Results The 3D relationship between visible structures seen on MRI was consistent with the intraoperative findings in all patients. All cases were divided into three groups by the degree of trigeminal nerve encroachment by SPV. Statistical analysis revealed that the distance from the SPV to the trigeminal nerve was significantly different among the three groups. The diameter of the SPV also differed among the three groups.
Conclusion Preoperative 3D imaging provides reliable and detailed information about the intraoperative anatomical relationship between the trigeminal nerve and the SPV. This evaluation is useful for preoperative planning.