bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibody–drug conjugates in patients with advanced/metastatic HER2-low-expressing breast cancer: a systematic review and meta-analysis
Authors: Michelon Isabella , Dacoregio Maria Inez , Vilbert Maysa , Priantti Jonathan , do Rego Castro Caio Ernesto , Vian Lucas , Tarantino Paolo , Azambuja Evandro de , Cavalcante Ludimila 
Published: November 2024

Background: Until recently, targeted therapies have failed to benefit patients with human epidermal growth factor receptor 2 (HER2)-low-expressing breast cancer (BC). Nevertheless, antibody–drug conjugates (ADCs) have reshaped their prognosis. Objectives: We performed a systematic review and meta-analysis to assess the effectiveness of ADCs in patients with HER2-low advanced/metastatic (a/m) BC. Design: This study is a systematic review and meta-analysis. Data sources: We searched PubMed, Embase, and Cochrane databases as well as the American Society of Clinical Oncology, European Society for Medical Oncology, and San Antonio Breast Cancer Symposium conference proceedings. Methods: Studies evaluating ADCs (trastuzumab deruxtecan (T-DXd), sacituzumab govitecan (SG), MRG002, and RC48-ADC) in patients with HER2-low a/mBC were included. We used R software (v.4.2.2) and random effects models for all analyses. Heterogeneity was assessed using the I2 test. Results: Overall, 14 studies were included (five real-world studies and nine clinical trials (CTs)), with 2883 HER2-low a/mBC patients: 808 received treatment of physician’s choice (TPC), and 2075 ADCs. Most were treated with T-DXd ( n = 1691), followed by SG ( n = 310), MRG002 ( n = 56), and RC48-ADC ( n = 18). Patients treated with T-DXd achieved a significantly higher objective response rate (ORR), disease control rate (DCR), and clinical benefit rate (CBR) than those receiving other ADCs. In the pooled analysis of four randomized CTs, ADCs statistically prolonged progression-free survival ( n = 1828, hazard ratio (HR) 0.50, 95% confidence interval (CI) 0.36–0.68, I2 = 82%, p < 0.001) and overall survival ( n = 1546, HR 0.70, 95% CI 0.57–0.86, I2 = 43%, p < 0.001) compared with TPC. Patients on ADCs also achieved a greater antitumor response than TPC, including better ORR (odds ratio (OR), 3.7, 95% CI 2.5–5.6, I2 = 59%, p < 0.001), DCR (OR, 2.7, 95% CI 2.1–3.5, I2 = 0%, p < 0.001), and CBR (OR, 3.6, 95% CI 2.6–5.2, I2 = 56%, p < 0.01). Conclusion: Our systematic review and meta-analysis confirms the efficacy of ADCs in HER2-low a/m BC patients over TPC. Future studies should focus on bringing ADCs into earlier lines of therapy in this population. Trial registration: This study was registered in PROSPERO (CRD42024452962).
Cross-Cultural Adaptation and Validation of Foot and Ankle Ability Measure (FAAM) for Arabic-Speaking Patients
Authors: Almousa Sulaiman A. 
Published: November 2024

Background The original English Foot and Ankle Ability Measure (FAAM) is widely adopted to track changes in foot and ankle function. The aim of this study is to translate it to an Arabic version of FAAM (FAAM-A), and then to assess the validity and reliability of the FAAM-A version. Methodology One hundred five consecutive Arabic-speaking participants with different foot and ankle pathologies completed the FAAM-A. Fifty-six males and 49 females with a mean age of 39.8 years; 78.1% respondents have completed high school or higher. Convergent and divergent validities were assessed using correlation coefficients between the FAAM-A subscales and the short form-36 (SF-36), physical function (PF), physical component summary (PCS), mental health (MH), and mental component summary (MCS). Cronbach’s alpha was calculated to assess internal consistency. A subgroup of 20 participants filled the FAAM-A twice to test for test-retest reliability, using intraclass correlation coefficients (ICCs) and minimal detectable changes (MDCs). Results The average FAAM-A was 55.32 and 43.23 for the activity of daily life (ADL) and Sports subscales, respectively. FAAM-A ADL subscale had a moderate correlation with the PF, 0.565; PCS, 0.546; and MCS, 0.447; and a low correlation with MH, 0.34. The sports subscale had a moderate correlation with the PF (0.529), PCS (0.513), and a low correlation with MCS (0. 395) and MH (0. 297). Cronbach’s alpha was 0.97 and 0.95 for ADL and sports subscales, respectively. Intraclass correlation coefficient was 0.99 for both subscales. Minimal detectable change at a 95% confidence level was 6.81 and 8.19 for the ADL and sports subscales, respectively. Conclusion The FAAM-A is proven to be valid and reliable instrument for assessing foot and ankle disability. It is an effective tool for clinical assessment and research within Arabic-speaking populations. Level of Evidence: Level II, Prospective cohort study
The impact of granulocyte colony-stimulating factor and decitabine-containing conditioning in myelodysplastic syndrome patients with iron overload undergoing allogeneic hematopoietic stem cell transplantation: a retrospective study
Authors: Zhao Wenshu , Zeng Xiangzong , Pan Danqi , Xuan Li , Fan Zhiping , Huang Fen , Xu Na , Sun Jing , Liu Qifa , Dai Min 
Published: November 2024

Background: Iron overload is considered an unfavorable prognosis in myelodysplastic syndrome (MDS) even in those undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). Although iron chelation therapy has improved the prognosis of these patients to some extent, the effect has not yet been satisfactory. Objectives: This study aimed to investigate the impact of granulocyte colony-stimulating factor and decitabine (G-DAC)-containing conditioning in iron-overloaded MDS patients undergoing allo-HSCT. Design: This was a retrospective study. Methods: One hundred and ninety-seven patients were enrolled in this retrospective study. Based on the level of serum ferritin (SF) and conditioning regimen, all patients enrolled were divided into four groups: SF < 1000 µg/L with G-DAC conditioning (cohort 1), SF < 1000 µg/L with non-G-DAC conditioning (cohort 2), SF ⩾ 1000 µg/L with G-DAC conditioning (cohort 3), and SF ⩾ 1000 µg/L with non-G-DAC conditioning (cohort 4). The clinical features and prognosis of the four groups were analyzed. Results: Significant differences in the 2-year overall survival (OS), disease-free survival (DFS), and the cumulative incidence of non-relapse mortality (NRM) were observed between the four groups. Multivariate analysis revealed that SF ⩾ 1000 µg/L was a risk factor for OS, DFS, and NRM while G-DAC-containing conditioning was a protective factor. Intriguingly, when cohort 1 to cohort 4 were included in the multivariate analysis, only cohort 4 was a risk factor for OS, DFS, and NRM, cohort 3 had no difference in prognosis compared with patients with SF < 1000 µg/L. Conclusion: The poor prognosis of patients with iron overload may be overcome by G-DAC-containing conditioning partly.
Synchronized application of closed-loop NMES and precision tACS in post-stroke hand rehabilitation: a protocol of neurorehabilitation trial
Authors: Tashiro Syoichi , Takemi Mitsuaki , Yamada Shin , Tsuji Tetsuya 
Published: November 2024

Background: Severe upper extremity paresis due to stroke is a significant clinical sequela. Neuromuscular electrical stimulation (NMES)-based rehabilitation has demonstrated promising results along with cortical plasticity. Transcranial alternating current stimulation (tACS) has gained attention due to its unique ability to entrain endogenous oscillatory brain rhythms with injected AC frequency, offering the potential for modifying brain conditions to enhance rehabilitative interventions. Because repetitive motor execution in rehabilitation training requires a smooth transition of the brain state despite often being impaired secondary to stroke, combining NMES and tACS may offer better treatment efficacy. Aim: This study proposes a phase I/II trial of an outpatient comprehensive rehabilitative treatment combining the integrated volitional-control electrical stimulation (IVES), a closed-loop NMES, and the timing-specified focal tACS in individualized beta frequency (dynamic-precision tACS) targeting severe hand paresis in patients with chronic stroke, aiming to demonstrate the feasibility of combination treatment. Design: Double-blind randomized cross-over trial. Methods: The repetitive facilitative finger extension training utilizing closed-loop NMES is combined with dynamic-precision tACS on the primary motor cortex to assist post-movement beta-rebound. Together with regular occupational therapy, we propose a comprehensive outpatient neurorehabilitative regimen. Here, a total of 10 sessions will be conducted using a cross-over design using real and sham tACS. Analysis: The perception and fatigue from stimulation will be investigated as the primary outcomes. The efficacy of improving sensorimotor function and their background physiological mechanisms will be evaluated as the secondary outcomes. Discussion: This phase I/II trial will be the first to combine tACS and neurorehabilitation using functional electrical stimulation. A weekly outpatient protocol with cheap devices may offer a new treatment paradigm toward functional recovery for chronic stroke patients with severe upper extremity paresis. Ethics and trial registration: This study was approved by the Ethics Committee of Kyorin University Faculty of Medicine (814-01). The trial was registered in a public database: UMIN000048274.
Social disparities on PrEP use and awareness among sexual and gender minorities using smartphones in India
Authors: Agarwal Harsh , Yeatts Karin , Chung Stephanie R. , Harrison-Quintana Jack , Torres Thiago S. 
Published: November 2024

Background: Pre-exposure prophylaxis (PrEP) is a safe and effective HIV prevention strategy. However, in countries such as India where PrEP is driven by the private healthcare system and there is no centralized reporting, it is unknown which populations benefit from PrEP and which populations are being left behind. Objectives: We examined and characterized PrEP use and awareness among the sexual and gender minorities using smartphones in India and found measures of association of PrEP use. Design: This is a cross-sectional study design. Methods: We used Grindr—a widely used geosocial mobile application—to conduct a national cross-sectional survey in India, including respondents who were 18 years or older and reported sex with men (those who identified as cis-gender females were excluded). We examined overall PrEP awareness and PrEP use, then calculated adjusted prevalence odds ratio and 95% confidence intervals to understand PrEP use correlation with socio-behavioral factors. Results: Out of the total of 3116 eligible participants, 30.3% ( N = 947) were aware of PrEP and 3.1% ( N = 97) reported current PrEP use. Our multivariate regression model found that there was a statistically significant association of PrEP use with higher income, being employed, preferred language as English for survey, relationship status as single, and use of party drugs. At the same time, there was a statistically significant association of PrEP awareness with age group, having higher education as a graduate or above, higher income, use of party drugs, and multiple sexual partners. Conclusion: We found overall low awareness and low PrEP use in our cross-sectional sample. PrEP use and awareness were higher among those who belonged to higher-income groups. Including PrEP in existing programmatic interventions by government and NGOs may contribute to PrEP scale-up, which is urgent to stop the HIV epidemic in India.
Upper limb disabilities and associated factors among breast cancer survivors: A quantitative cross-sectional study
Authors: John Davidson Okwudili , Amaeze Augustine , Ransome Onyinyechi Peace , John Jeneviv Nene , Okezue Obinna Chinedu , Iyare Oluwatoyin , Ugwu Ngozi Immaculata 
Published: November 2024

Objectives: Complications following breast cancer treatment result in chronic upper limb disabilities. To plan an informed and effective rehabilitation for timely intervention to prevent, mitigate, or manage the functional impairments for breast cancer survivors, especially in settings with limited resources, the burden of upper limb disabilities needs to be ascertained. This study examined upper limb disabilities and associated factors among breast cancer survivors. Methods: This cross-sectional quantitative study recruited 60 breast cancer survivors using purposive sampling method. Text messages and face-to-face interactions were used to inform participants about the study. Disability of the Arm, Shoulder and Hand questionnaire was used to ascertain the upper limb disabilities. To determine lymphedema, tape measurements of upper limb circumference were translated into a limb volume with the geometric formula for a truncated cone. Visual analogue scale, hand dynamometer, and goniometer were used to measure pain, grip strength, and shoulder range of movement, respectively. Descriptive statistics were used to summarize data. Linear regression was used to determine the correlation between upper limb disabilities and selected variable. Alpha level was set at p < 0.05. Result: The prevalence of upper limb disabilities was 73%. Pain, lymphedema, shoulder range of movement, and grip strength showed strong correlation with upper limb disabilities. Pain and lymphedema increased by around 0.095 and 0.061 units, respectively, for every unit increase in disability. Conversely, there was a decrease of 1.394, 0.770, 0.285, and 0.045 in shoulder flexion, shoulder abduction, grip strength, and body mass index, respectively, for every unit increase in disability. Conclusion: Upper limb disabilities had high prevalence and significantly correlated with every variable that was examined. The high prevalence of upper limb disabilities and their interaction with related variables calls to action for routine screening and prompt intervention to identify, prevent, or manage upper limb functional impairments in breast cancer survivors.
Renal function outcomes in living kidney donors in a transplant center in Colombia
Authors: Torres Rodolfo , Reina Maricely , Montero Camilo , Tunjano Andres , Andrade David , Mancera Valeria , Amaya Maria , Arias Lizeth , Castellanos Laura , Vanegas Valentina 
Published: November 2024

Introduction: Living kidney donation is currently low in Colombia, and this is associated with the lack of knowledge of the risks and renal function outcomes of potential donors; there are no studies that evaluate these outcomes. The objective of this study is to evaluate the outcomes of renal function, the incidence of metabolic diseases, arterial hypertension, as well as the finding of albuminuria and/or proteinuria in living kidney donors with a 2-year follow-up post donation. Methods: Observational study in living kidney donor patients, in which renal function outcomes were evaluated between the predonation period and up to 24 months postdonation. Results: Ninety-one patients were included, with a median predonation glomerular filtration rate of 98 ml/min/1.73 m2, interquartile range (90.5–109), and 24-month postdonation of 66.3 ml/min/1.73 m2 interquartile range (57.9–75). A total of 60.26% of the population was in stage 2 at the end of follow-up and no patient had a glomerular filtration rate less than 30 ml/min/1.73 m2 or required renal support therapy. Conclusion: A living donor evaluation process based on risk factor stratification and adequate assessment of renal function was found to generate safe renal function outcomes both in the perioperative period and in medium- and long-term follow-up.
Exploring ideal nasal aesthetics in Thailand: Trends and participants’ awareness of rhinoplasty
Authors: Kaewsomnuk Chunanuch , Suetrong Surapol , Reechaipichitkul Wisoot , Chainansamit Seksun , Kasemsiri Pornthep , Komany Kan , Piromchai Patorn 
Published: November 2024

Backgrounds: Beauty standards vary across cultures and regions. To date, no research has identified ideal nasal profiles for Thai individuals. This study aims to determine the preferred nasal aesthetics among the Thai population. Methods: This cross-sectional study employed an online self-assessment questionnaire to investigate the ideal nasal profiles among Thai adults aged 18–60. Data were collected from participants across all regions of Thailand and included demographic information, rhinoplasty intentions, and perceptions of ideal nasal features. Results: Of the 1452 respondents, 35.74% (519 individuals) have either undergone or plan to undergo rhinoplasty, while the remaining 64.26% (933 individuals) expressed either no interest or uncertainty about the procedure. The ideal nasolabial angle was found to be between 97 and 108 degrees for males and 88 to 98 degrees for females, respectively. Male participants in the rhinoplasty and control groups exhibited statistically significant differences in nasolabial angle ( p = 0.017), nasofrontal angle ( p < 0.001), and alar-intercanthal distance ratio ( p < 0.001). Female participants demonstrated statistically significant differences between groups in nasofacial angle ( p < 0.001) and nasolabial angle ( p < 0.001). Conclusions: Our study identified distinct ideal nasal profiles among the Thai general population and those considering rhinoplasty. Our findings differed from the neoclassical orbitonasal proportion (canon V). These findings offer insights into contemporary Thai beauty standards for the nose, which can inform clinical decision-making in nasal treatment.
“UPDATE: I’m pregnant!”: Inferring global downloads and reasons for using menstrual tracking apps
Authors: Rampazzo Francesco , Raybould Alyce , Rampazzo Pietro , Barker Ross , Leasure Douglas 
Published: November 2024

Objective The market for smartphone apps tracking menstrual cycles has expanded in recent years. These apps market themselves as empowering users to achieve their reproductive goals and maximize the chance of pregnancy. This paper presents the first open-access quantification of menstrual tracking app downloads globally, covering both the Global North and South. We also explore macro and micro-level reasons for app usage, testing national associations with downloads and analyzing user reviews. Methods We use data on app installations, reviews, and ratings from the Google Play Store and Apple App Store to estimate global app downloads using a Bayesian model. We perform regressions to test for national predictors of use, and multilingual topic models to analyze/cluster reviews left by users to understand individual reasons for use. Results We find that the majority of downloads are for three apps: Clue, Flo, and Period Tracker. Higher modern contraceptive prevalence and internet access are associated with more downloads, while low-income countries tend to have fewer. In low-income countries, a higher unmet need for family planning and total fertility rate are associated with more downloads. Individual reviews reveal the most common reasons for use are menstrual cycle tracking, achieving a pregnancy, community engagement, and avoiding pregnancy. Conclusion Existing research on menstrual tracking apps is largely confined to the Global North, but our study finds the use of these apps to be as prevalent throughout the Global South. Future research needs to urgently understand the implications these apps could have in a diversity of contexts.
Inflammatory Waldenström macroglobulinemia is associated with clonal hematopoiesis: a multicentric cohort
Authors: Debureaux Pierre-Edouard , Poulain Stéphanie , Harel Stéphanie , Passet Marie , Templé Marie , Friedrich Chloé , Forgeard Nathalie , Elessa Dikelele , Plas William , Chat Laureen , Lazarian Grégory , Willems Lise , Royer Bruno , Talbot Alexis , Vaugeois Tristan , Theves Floriane , Terré Alexandre , Brignier Anne , Malphettes Marion , Krzisch Daphné , Frenzel Laurent , Davi Frédéric , Bravetti Clotilde , Nguyen-Khac Florence , Dupuis Jehan , Cuccuini Wendy , Bouscary Didier , Hermine Olivier , Roos-Weil Damien , Kosmider Olivier , Clappier Emmanuelle , Espéli Marion , Balabanian Karl , Arnulf Bertrand 
Published: November 2024

Abstract
Inflammatory form of Waldenström macroglobulinemia (iWM) predicts outcomes after immuno-chemotherapy and Bruton tyrosine kinase inhibitors, but its origin is unknown. Here, we unravel increased clonal hematopoiesis in patients with iWM (61% vs 23% in noninflammatory WM), suggesting a contribution of environmental cells to iWM.
Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3
Authors: Chuang Hsiu-Chun , Li Ruidong , Huang Huang , Liu Szu-Wen , Wan Christine , Chaudhuri Subhra , Yue Lili , Wong Terence , Dominical Venina , Yen Randy , Ngo Olivia , Bui Nam , Stoppler Hubert , Yi Tangsheng , Suthram Silpa , Li Li , Sun Kai-Hui 
Published: November 2024

AbstractWe developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, scalability, and method reproducibility. This workflow consistently produces high-quality data with high cell capture efficiency and gene detection sensitivity. In a rigorous comparison with the 10X platform using human primary CD4 + T-cells, HT Smart-seq3 demonstrated higher cell capture efficiency, greater gene detection sensitivity, and lower dropout rates. Additionally, when sufficiently scaled, HT Smart-seq3 achieved a comparable resolution of cellular heterogeneity to 10X. Notably, through T-cell receptor (TCR) reconstruction, HT Smart-seq3 identified a greater number of productive alpha and beta chain pairs without the need for additional primer design to amplify full-length V(D)J segments, enabling more comprehensive TCR profiling across a broader range of species. Taken together, HT Smart-seq3 overcomes key technical challenges, offering distinct advantages that position it as a promising solution for the characterization of single-cell transcriptomes and immune repertoires, particularly well-suited for low-input, low-RNA content samples.
A comprehensive study of Z-DNA density and its evolutionary implications in birds
Authors: Wang Yu-Ren , Chang Shao-Ming , Lin Jinn-Jy , Chen Hsiao-Chian , Lee Lo-Tung , Tsai Dien-Yu , Lee Shih-Da , Lan Chung-Yu , Chang Chuang-Rung , Chen Chih-Feng , Ng Chen Siang 
Published: November 2024

Abstract
Background
Z-DNA, a left-handed helical form of DNA, plays a significant role in genomic stability and gene regulation. Its formation, associated with high GC content and repetitive sequences, is linked to genomic instability, potentially leading to large-scale deletions and contributing to phenotypic diversity and evolutionary adaptation.

Results
In this study, we analyzed the density of Z-DNA-prone motifs of 154 avian genomes using the non-B DNA Motif Search Tool (nBMST). Our findings indicate a higher prevalence of Z-DNA motifs in promoter regions across all avian species compared to other genomic regions. A negative correlation was observed between Z-DNA density and developmental time in birds, suggesting that species with shorter developmental periods tend to have higher Z-DNA densities. This relationship implies that Z-DNA may influence the timing and regulation of development in avian species. Furthermore, Z-DNA density showed associations with traits such as body mass, egg mass, and genome size, highlighting the complex interactions between genome architecture and phenotypic characteristics. Gene Ontology (GO) analysis revealed that Z-DNA motifs are enriched in genes involved in nucleic acid binding, kinase activity, and translation regulation, suggesting a role in fine-tuning gene expression essential for cellular functions and responses to environmental changes. Additionally, the potential of Z-DNA to drive genomic instability and facilitate adaptive evolution underscores its importance in shaping phenotypic diversity.

Conclusions
This study emphasizes the role of Z-DNA as a dynamic genomic element contributing to gene regulation, genomic stability, and phenotypic diversity in avian species. Future research should experimentally validate these associations and explore the molecular mechanisms by which Z-DNA influences avian biology.