bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trends in caries experience in the permanent dentition in Germany 1997–2014, and projection to 2030: Morbidity shifts in an aging society
Authors: Jordan Rainer A. , Krois Joachim , Schiffner Ulrich , Micheelis Wolfgang , Schwendicke Falk 
Published: April 2019

AbstractThe aims of this study were to assess the trends in dental caries experience in the permanent dentition (i.e., the number of decayed, missing, or filled teeth, DMFT) in Germany from 1997–2014 and to project caries experience to 2030. Components of caries experience (decayed teeth, DT, missing teeth, MT, filled teeth, FT) from repeated waves (1997, 2005, 2014) of the nationally representative German Oral Health Studies were analyzed in 12-, 35–44-, and 65–74-year-olds. Weighted means were interpolated cross-sectionally by fitting piecewise-cubic spline-curves and were then subjected to longitudinal regression and combined with population estimates. In 1997, children (12-year-olds) had a mean caries experience (decayed, missing, filled teeth, DMFT) of 1.7 teeth; this experience decreased to 0.5 teeth in 2014. For 2030, an experience of 0.2 teeth is projected. In adults (35–44-year-olds), a decrease was recorded (1997: 16.1 teeth; 2014: 11.2 teeth). This decrease is expected to continue until 2030 (to 7.7 teeth). Similarly, in seniors (65–74-year-olds), a decrease was recorded (1997: 23.6 teeth; 2014: 17.7 teeth); this decrease is expected to continue until 2030 (to 14.9 teeth). While the number of missing teeth has decreased consistently across age groups, the number of filled and decayed teeth has increased in seniors and is expected to continue to increase. The cumulative caries experience has decreased from 1.1 billion DMFT in 2000 to 867 million in 2015 and is expected to decrease to 740 million in 2030. Caries experience in the permanent dentition has been decreasing substantially, mainly due to a decrease in missing teeth. Younger age groups also show fewer decayed and filled teeth, while in older groups, restorative needs have not decreased, as more teeth are retained. Concepts for addressing the emanating morbidity shifts are required.
Reconfigurable and optically transparent microwave absorbers based on deep eutectic solvent-gated graphene
Authors: Grande Marco , Bianco Giuseppe Valerio , Perna Filippo Maria , Capriati Vito , Capezzuto Pio , Scalora Michael , Bruno Giovanni , D’Orazio Antonella 
Published: April 2019

AbstractElectrolytically tunable graphene “building blocks” for reconfigurable and optically transparent microwave surfaces and absorbers have been designed and fabricated by exploiting Deep Eutectic Solvents (DESs). DESs have been first explored as electrolytic and environmentally friendly media for tuning sheet resistance and Fermi level of graphene together with its microwave response (reflection, transmission and absorption). We consider the tunability of the reconfigurable surfaces in terms of transmittance, absorption and reflectance, respectively, over the X and Ku bands when the gate voltage is varied in the −1.4/+1.4 V range. The numerical simulations and experimental measurements also show the ability of the absorber, in the Salisbury screen configuration, to achieve near perfect absorption with a modulation of about 20%. These results could find applications in several technological fields, ranging from electromagnetic pollution to integrated multi-physical regulation systems, thereby helping the advance of the performance of microwave cloaking systems, stealth windows, frequency selective surfaces, modulators and polarizers.
A generalized model for combining dependent SNP-level summary statistics and its extensions to statistics of other levels
Authors: Svishcheva Gulnara R. 
Published: April 2019

AbstractHere I propose a fundamentally new flexible model to reveal the association between a trait and a set of genetic variants in a genomic region/gene. This model was developed for the situation when original individual-level phenotype and genotype data are not available, but the researcher possesses the results of statistical analyses conducted on these data (namely, SNP-level summary Z score statistics and SNP-by-SNP correlations). The new model was analytically derived from the classical multiple linear regression model applied for the region-based association analysis of individual-level phenotype and genotype data by using the linear compression of data, where the SNP-by-SNP correlations are among the explanatory variables, and the summary Z score statistics are categorized as the response variables. I analytically show that the regional association analysis methods developed within the framework of the classical multiple linear regression model with additive effects of genetic variants can be reformulated in terms of the new model without the loss of information. The results obtained from the regional association analysis utilizing the classical model and those derived using the proposed model are identical when SNP-by-SNP correlations and SNP-level statistics are estimated from the same genetic data.
Enrichment of periodontal pathogens from the biofilms of healthy adults
Authors: Naginyte Monika , Do Thuy , Meade Josephine , Devine Deirdre Ann , Marsh Philip David 
Published: April 2019

AbstractPeriodontitis is associated with shifts in the balance of the subgingival microbiome. Many species that predominate in disease have not been isolated from healthy sites, raising questions as to the origin of these putative pathogens. The study aim was to determine whether periodontal pathogens could be enriched from pooled saliva, plaque and tongue samples from dentally-healthy adult volunteers using growth media that simulate nutritional aspects of the inflamed subgingival environment. The microbiome was characterised before and after enrichment using established metagenomic approaches, and the data analysed bioinformatically to identify major functional changes. After three weeks, there was a shift from an inoculum in whichStreptococcus,Haemophilus,Neisseria,VeillonellaandPrevotellaspecies predominated to biofilms comprising an increased abundance of taxa implicated in periodontitis, includingPorphyromonas gingivalis,Fretibacterium fastidiosum,Filifactor alocis,Tannerella forsythia, and severalPeptostreptococcusandTreponemaspp., with concomitant decreases in health-associated species. Sixty-four species were present after enrichment that were undetectable in the inoculum, includingJonquetella anthropi,Desulfovibrio desulfuricansandDialister invisus. These studies support the Ecological Plaque Hypothesis, providing evidence that putative periodontopathogens are present in health at low levels, but changes to the subgingival nutritional environment increase their competitiveness and drive deleterious changes to biofilm composition.
Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer’s disease patients
Authors: Nilsson Jonas , Brinkmalm Gunnar , Ramadan Sherif , Gilborne Lisa , Noborn Fredrik , Blennow Kaj , Wallin Anders , Svensson Johan , Abo-Riya Mohamed A. , Huang Xuefei , Larson Göran 
Published: April 2019

AbstractAn early pathological hallmark of Alzheimer’s disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP). We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15–20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients. We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls. Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group. The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.
Ovarian cancer cell lines derived from non-serous carcinomas migrate and invade more aggressively than those derived from high-grade serous carcinomas
Authors: Hallas-Potts Amelia , Dawson John C. , Herrington C. Simon 
Published: April 2019

AbstractThe term ovarian cancer describes a heterogeneous group of tumours that grow in the ovary but are not necessarily of ovarian origin. Recent genomic analysis has shown that many of the most commonly used ovarian cancer cell lines have been mischaracterised, leading to erroneous conclusions and a gap in the translation of laboratory research into novel treatments for patients. Here, we use 10 epithelial ovarian cancer cell lines to investigate 2D migration, cell cycle parameters and 3D invasion behaviour into different substrates and find significant differences between the behaviours of cell lines from different origins. Cell lines derived from non-serous carcinomas migrated more quickly and were more likely to invade into Matrigel and collagen I substrates than cell lines derived from high-grade serous carcinomas. However not all cell lines derived from non-serous carcinomas exhibited similar invasive behaviour. These findings may reflect differences in the behaviour of the primary tumour types from which the cell lines were derived, given that high-grade serous carcinomas typically expand and spread over peritoneal surfaces. These results provide the basis of an in vitro model for identifying differences between ovarian cancer tumour types.
Four novel polymorphisms in long non-coding RNA HOTTIP are associated with the risk and prognosis of colorectal cancer
Authors: Lv Zhi , Xu Qian , Sun Liping , Wen Jing , Fang Xinxin , Xing Chengzhong , Yuan Yuan 
Published: April 2019

Abstract
Background: The role of long non-coding RNA (lncRNA) HOXA transcript at the distal tip (HOTTIP) as an oncogene in varieties of human cancer including colorectal cancer (CRC) has been extensively researched. The expression and function of lncRNAs could be affected by single nucleotide polymorphisms (SNPs), which are associated with cancer susceptibility and prognosis. However, no investigation has focused on the association between HOTTIP SNPs and CRC. The aim of the present study was to explore the association of polymorphisms in the lncRNA HOTTIP gene with CRC risk and prognosis. Methods: A total of 1848 subjects were enrolled in our study, including 884 CRC cases and 964 controls. Genotyping for five HOTTIP tagSNPs (rs3807598, rs17501292, rs2067087, rs17427960, and rs78248039) was performed by applying Kompetitive allele specific PCR (KASP). Results: The results showed three SNPs (rs3807598, rs2067087, and rs17427960) were associated with enhanced CRC risk both in overall and stratified analysis. One polymorphism, rs17501292, could improve the overall survival (OS) of CRC patients in the tumor of ulcerative/invasive-type subgroup. Conclusion: These findings suggest HOTTIP SNPs could potentially be predictive biomarkers for CRC risk and prognosis. The present study provides clues for further exploration of novel lncRNA-based genetic biomarkers to predict CRC susceptibility as well as clinical outcome.
Hemogram and iron indices in renal anemia and the amelioration with Carica papaya leaf extract applied on albino rat model
Authors: Gheith Ibtsam , El-Mahmoudy Abubakr 
Published: April 2019

Abstract
The present study was designed to look at the hematological disorders in gentamicin nephrotoxicity model, as kidney is considered as one of the hemopoietic organs. In a previous study, novel and classical kidney injury biomarkers were utilized to evaluate the nephroprotective potential of Carica papaya leaf extract (CPLE) in the same model in albino rats. Gentamicin (100 mg/kg, subcutaneously, for 21 consecutive days) resulted in significant decreases in red blood cell (RBC) count, hemoglobin concentration (HGB), and packed cell volume (PCV) value, with minimal alterations in erythrocytic indices. Leucogram showed leukocytosis, granulocytosis, and thrombocytopenia. Erythropoietin (EPO) levels were also drastically decreased by the end of the experimental course. Serum iron, unsaturated iron-binding capacity (UIBC), total iron binding capacity (TIBC), transferrin saturation %, and serum transferrin concentration values were significantly decreased in contrast to ferritin, which was increased. When concurrently administered with gentamicin, CPLE (150 and 300 mg/kg, orally via gastric tube, for 21 days) significantly protected against the drastic effects of the former on the blood profile with improving potentials on erythrogram, leukogram, thrombocytes, EPO, iron and its indices, in a dose-dependent manner. These data may suggest CPLE as an appreciated blood homeostatic and nephroprotective agent from a natural source that could be a good remedy in conditions associated with blood disorders.
Acetylation & Co: an expanding repertoire of histone acylations regulates chromatin and transcription
Authors: Barnes Claire E. , English David M. , Cowley Shaun M. 
Published: April 2019

Abstract
Packaging the long and fragile genomes of eukaryotic species into nucleosomes is all well and good, but how do cells gain access to the DNA again after it has been bundled away? The solution, in every species from yeast to man, is to post-translationally modify histones, altering their chemical properties to either relax the chromatin, label it for remodelling or make it more compact still. Histones are subject to a myriad of modifications: acetylation, methylation, phosphorylation, ubiquitination etc. This review focuses on histone acylations, a diverse group of modifications which occur on the ε-amino group of Lysine residues and includes the well-characterised Lysine acetylation. Over the last 50 years, histone acetylation has been extensively characterised, with the discovery of histone acetyltransferases (HATs) and histone deacetylases (HDACs), and global mapping experiments, revealing an association of hyperacetylated histones with accessible, transcriptionally active chromatin. More recently, there has been an explosion in the number of unique short chain ‘acylations’ identified by MS, including: propionylation, butyrylation, crotonylation, succinylation, malonylation and 2-hydroxyisobutyrylation. These novel modifications add a range of chemical environments to histones, and similar to acetylation, appear to accumulate at transcriptional start sites and correlate with gene activity.
Disordered domains in chromatin-binding proteins
Authors: Watson Matthew , Stott Katherine 
Published: April 2019

Abstract
Chromatin comprises proteins, DNA and RNA, and its function is to condense and package the genome in a way that allows the necessary transactions such as transcription, replication and repair to occur in a highly organised and regulated manner. The packaging of chromatin is often thought of in a hierarchical fashion starting from the most basic unit of DNA packaging, the nucleosome, to the condensation of nucleosomal ‘beads on a string’ by linker histones to form the 30-nm fibre and eventually large chromatin domains. However, a picture of a more heterogeneous, dynamic and liquid-like assembly is emerging, in which intrinsically disordered proteins (IDPs) and proteins containing intrinsically disordered regions (IDRs) play a central role. Disorder features at all levels of chromatin organisation, from the histone tails, which are sites of extensive post-translational modification (PTM) that change the fate of the underlying genomic information, right through to transcription hubs, and the recently elucidated roles of IDPs and IDRs in the condensation of large regions of the genome through liquid–liquid phase separation.
CTCF: a Swiss-army knife for genome organization and transcription regulation
Authors: Braccioli Luca , de Wit Elzo 
Published: April 2019

Abstract
Orchestrating vertebrate genomes require a complex interplay between the linear composition of the genome and its 3D organization inside the nucleus. This requires the function of specialized proteins, able to tune various aspects of genome organization and gene regulation. The CCCTC-binding factor (CTCF) is a DNA binding factor capable of regulating not only the 3D genome organization, but also key aspects of gene expression, including transcription activation and repression, RNA splicing, and enhancer/promoter insulation. A growing body of evidence proposes that CTCF, together with cohesin contributes to DNA loop formation and 3D genome organization. CTCF binding sites are mutation hotspots in cancer, while mutations in CTCF itself lead to intellectual disabilities, emphasizing its importance in disease etiology. In this review we cover various aspects of CTCF function, revealing the polyvalence of this factor as a highly diversified tool for vertebrate genome organization and transcription regulation.
Analysis of braking intention based on fNIRS in driving simulation experiments
Authors: Zhu Lei , Li Shuguang , Li Yaohua , Wang Min , Zhang Chenyang , Li Yanyu , Yao Jin , Ji Hao 
Published: April 2019

Cooperative driving refers to a notion that advanced driver assistance system (ADAS) sharing the control with human driver and completing driving task together. One of the key technologies is that the ADAS can identify the driver's driving intention in real time to implement consistent driving decisions. Based on driving simulator (DS) and functional near‐infrared spectroscopy (fNIRS) experiments, this study established a model of driver's brake intention identification with machine‐learning algorithms and analysed cerebral cortex activity mechanism of the driver's brake intention with parametric test. This study suggested that the test accuracy of the model established here was 90.91%. Moreover, the activity in the Brodmann area 7 (BA7), BA17, and BA40 in cerebral cortex was significantly different between the driver with braking intention and those with driving at constant speed (p  < 0.05). The study presented here not only identified the driver's driving intention through fNIRS for the first time, but also analysed the brain activity of the drivers when he had the braking intention. It lays a foundation for the future research on the driving cognitive model about human perception and driver's driving intention identification.
Resilient wide‐area monitoring and protection scheme with IEEE Std. C37.118.1‐2011 criteria for complex smart grid system using phase diagram
Authors: Eissa Moustafa M. 
Published: April 2019

Challenges facing power system protection in a wide‐area system are latency and full coverage of a wide‐area disturbance. The complexity of large‐scale power system configurations has led to challenges in the design of coordination and operating systems for protection relays. Local measurements used for primary and backup protection cannot consider wide system disturbances. A new wide‐area‐monitoring‐system‐based primary protection is presented for a complex power system involving double and single lines. It is based on describing the non‐linear dynamic operation of the transmission lines during a fault in the form of a set of differential equations that are solved through paths movements in a phase diagram. The fault on the lines can be precisely recognised. The speed of the traditional communication media limits wide‐area monitoring as backup protection. This study presents the primary protection scheme for double and single circuits in a wide area for the first time based on fourth‐generation technology with low latency. The justification for applying the proposed scheme as primary protection in a wide‐area‐monitoring system is discussed. The number of relays on the studied configuration is reduced from 18 local relays to only 5 phasor measurement units for protecting the lines in the area.
Development of mobile electrical vehicle for EMI applications
Authors: Leuchter Jan , Quang Huy Dong , Popela Miroslav 
Published: April 2019

This paper presents the relevancy, design, and test results of a mobile electrical vehicle for the applications of testing and providing electromagnetic interferences in Wi‐Fi signal in the band 2.4–2.5 GHz. The mobile device includes power electronics supplying a magnetron device for the interference. Propulsion of the mobile device is implemented by using a system of stepper motors. The mobile device is controlled with the help of the microcontroller ATmega 16. For the interfering part, it is necessary to design the power electronics to supply the magnetron from the battery. The power electronics interface between a battery and magnetron must be able to transfer output battery voltage 12 VDC to the variable output 4000 VAC and power around 1 kW. The magnetron is used as a source of electromagnetic interference high‐frequency acting on targets, which operates in the band of Wi‐Fi signal. In addition, the system must be able to control the direction and power of EMI interferences. The investigations of the power‐efficiency of power inverter versus power density will be discussed and optimised here.