bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soil acidification in continuously cropped tobacco alters bacterial community structure and diversity via the accumulation of phenolic acids
Authors: Bai Yuxiang , Wang Ge , Cheng Yadong , Shi Puyou , Yang Chengcui , Yang Huanwen , Xu Zhaoli 
Published: August 2019

AbstractStudying the obstacles associated with continuous cropping is necessary for sustainable agricultural production. Phenolic acids play an important role in continuous cropping systems, although their mechanism of action in these systems remains unclear. Using High-performance Liquid Chromatography, we characterized the changes in phenolic acid contents in soils that had been continuously cropped with tobacco for different time periods and evaluated the interactions between soil physicochemical properties, bacterial community structure and diversity, and phenolic acids. Prolonged continuous cropping was associated with a significant increase in the content of phenolic acids and a significant decrease in soil pH and bacterial diversity. A significant negative correlation between pH and phenolic acids content was observed, suggesting that soil acidification potentially leads to the accumulation of phenolic acids. The Mantel test indicated that phenolic acids were positively associated with relative bacterial abundance (R = 0.480, P < 0.01), signifying that the accumulation of phenolic acids is a potential factor leading to changes in bacterial community structure. Continuous cropping lowered the soil pH, which stimulated phenolic acid accumulation and consequently altered the bacterial community structure and diversity, ultimately impacting tobacco plant growth.
Effect of addition of adjuvants on physical and chemical characteristics of Bt bioinsecticide mixture
Authors: Santos Cicero Antônio Mariano dos , Santos Renata Thaysa da Silva , Della’Vechia Jaqueline Franciosi , Griesang Fabiano , Polanczyk Ricardo Antônio , Ferreira Marcelo da Costa 
Published: August 2019

AbstractBacillus thuringiensis(Bt) is the main bacterium used in the formulation of bioinsecticides because it produces toxins and spores that are toxic to several orders of insects. The efficacy of Bt bioinsecticide is influenced by the quality of its application. The association with other crop protection products, such as adjuvants, can affect the physical and chemical parameters of the mixture. This study evaluated the physical and chemical parameters, volume median diameter (VMD), uniformity coefficient of droplets (SPAN), percentage of volume in drift droplets (%V <100 µm), contact angle, surface tension, potential of hydrogen (pH) and electrical conductivity (E.C.) of Bt bioinsecticides in concentrated suspension (SC), and wettable powder (WP) formulations associated with adjuvants. The largest droplet diameter and smallest values of drift droplets were found in the WP formulation with lower drift potential. The addition of mineral oil and surfactant to the mixtures of bioinsecticide reduced contact angle values and surface tension of the droplets, resulting in greater spreading of droplets in leaves. The addition of lecithin and propionic-acid-based adjuvants lowered the pH in both formulations. The adjuvants used in this study affected the physical and chemical characteristics of the mixtures, improving or impairing the quality of Bt bioinsecticide applications.
Transcriptional profiling of corneal stromal cells derived from patients with keratoconus
Authors: Sharif Rabab , Khaled Mariam L. , McKay Tina B. , Liu Yutao , Karamichos Dimitrios 
Published: August 2019

AbstractKeratoconus (KC) is a multi-factorial corneal ectasia with unknown etiology affecting approximately 1:2000 people worldwide. Dysregulated gene expression, using RNA-Seq technology, have been reported in KC corneal tissue. However, the differential expression of genes, in KC corneal stromal cells have been widely ignored. We utilized mRNA-Seq to analyze gene expression in primary human corneal stromal cells derived from five non-Keratoconus healthy (HCF) and four Keratoconus (HKC) donors. Selected genes were further validated using real time PCR (RT-PCR). We have identified 423 differentially expressed genes with 187 down- and 236 up-regulated in KC-affected corneal stromal cells. Gene ontology analysis using WebGestalt indicates the enrichment of genes involved in cell migration, extracellular matrix, adherens junction, and MAPK signaling. Our protein-protein interaction network analysis identified several network seeds, such as EGFR, NEDD4, SNTA1, LGALS3BP, HSPB1, SDC2, MME, and HIF1A. Our work provides an otherwise unknown information on the transcriptional changes in HKCs, and reveals critical mechanisms of the cellular compartment. It also highlights the importance of human-based in vitro studies on a disease that currently lacks strong biomarkers and animal models.
Novel computational model of gastrula morphogenesis to identify spatial discriminator genes by self-organizing map (SOM) clustering
Authors: Mori Tomoya , Takaoka Haruka , Yamane Junko , Alev Cantas , Fujibuchi Wataru 
Published: August 2019

AbstractDeciphering the key mechanisms of morphogenesis during embryonic development is crucial to understanding the guiding principles of the body plan and promote applications in biomedical research fields. Although several computational tissue reconstruction methods using cellular gene expression data have been proposed, those methods are insufficient with regard to arranging cells in their correct positions in tissues or organs unless spatial information is explicitly provided. Here, we report SPRESSO, a new in silico three-dimensional (3D) tissue reconstruction method using stochastic self-organizing map (stochastic-SOM) clustering, to estimate the spatial domains of cells in tissues or organs from only their gene expression profiles. With only five gene sets defined by Gene Ontology (GO), we successfully demonstrated the reconstruction of a four-domain structure of mid-gastrula mouse embryo (E7.0) with high reproducibility (success rate = 99%). Interestingly, the five GOs contain 20 genes, most of which are related to differentiation and morphogenesis, such as activin A receptor and Wnt family member genes. Further analysis indicated that Id2 is the most influential gene contributing to the reconstruction. SPRESSO may provide novel and better insights on the mechanisms of 3D structure formation of living tissues via informative genes playing a role as spatial discriminators.
Demonstration of Container Effects on Recognition Process of Liquids Using a Ring-Resonator Measurement Method
Authors: Ozturk Turgut 
Published: August 2019

AbstractBefore separating or classifying hazardous/unsafe liquids, a system must be identified which can best measure certain liquids. The preferred measurement system can be useful, quick response, rapid measurement and so on. However, these features are not sufficient to classify the liquids in different containers. Especially at security points, containers of liquids carried by people may be different. Therefore, it has been investigated whether classification can be made by using the response of different containers to electromagnetic radiation. When the ring resonator measurement method is used in the 1–1.4 GHz frequency range, a successful separation process is carried out, even if the containers were made of different materials. Nevertheless, a well-known k-means algorithm has been used to analyze the measurement results of selected liquids.
Key evolutionary events in the emergence of a globally disseminated, carbapenem resistant clone in the Escherichia coli ST410 lineage
Authors: Feng Yu , Liu Lu , Lin Ji , Ma Ke , Long Haiyan , Wei Li , Xie Yi , McNally Alan , Zong Zhiyong 
Published: August 2019

AbstractThere is an urgent need to understand the global epidemiological landscape of carbapenem-resistant Escherichia coli (CREC). Here we provide combined genomic and phenotypic characterization of the emergence of a CREC clone from the ST410 lineage. We show that the clone expands with a single plasmid, within which there is frequent switching of the carbapenemase gene type between blaNDM and blaOXA-181 with no impact on plasmid stability or fitness. A search for clone-specific traits identified unique alleles of genes involved in adhesion and iron acquisition, which have been imported via recombination. These recombination-derived allelic switches had no impact on virulence in a simple infection model, but decreased efficiency in binding to abiotic surfaces and greatly enhanced fitness in iron limited conditions. Together our data show a footprint for evolution of a CREC clone, whereby recombination drives new alleles into the clone which provide a competitive advantage in colonizing mammalian hosts.
NMDA receptors and L-arginine/nitric oxide/cyclic guanosine monophosphate pathway contribute to the antidepressant-like effect of Yueju pill in mice
Authors: Wang Wei , Zhou Tong , Jia Rong , Zhang Hailou , Zhang Yi , Wang Chunxiu , Dong Yuwei , Wang Jianghui , Sheng Li , Wu Haoxin , Chen Gang , Xue Wenda 
Published: August 2019

Abstract
The present study aims to evaluate the involvement of N-methyl-d-aspartate receptor and nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) system in antidepressant-like effects of Yueju pill (YJ), a Chinese herbal medicine. The immobility time in tail suspension test (TST) and forced swim test (FST) was used to assess the antidepressant effects. Prior administration of L-arginine (750 mg/kg, intraperitoneal [i.p.]), a NO synthase substrate that enhances NO signaling or sildenafil (5 mg/kg, i.p.), a phosphodiesterase 5 inhibitor that enhances cGMP, blunted the antidepressant-like activity of YJ (2.7 g/kg, i.g.). Co-treatment of ineffective dose of YJ (1.35 g/kg, i.g.) with one of the reagents that suppress the NO/cGMP signaling, including methylene blue (10 mg/kg, i.p.), an inhibitor of NO synthase; 7-NI (7-nitroinidazole, 30 mg/kg, i.p.), an nNOS specific inhibitor; L-NAME (10 mg/kg, i.p.), a non-specific inhibitor of NO synthase; and MK-801 (0.05 mg/kg, i.p.), an NMDA receptor antagonist, reduced the immobility time in TST and FST, compared with those in vehicle or single drug treatment groups. Neither above drugs alone or co-administrated with YJ affected locomotor activity or anxiety behavior in open field test. Thus, our results suggest that the antidepressant-like action of YJ may depend on the inhibition of NMDA/NO/cGMP pathway.
Fossil group origins
Authors: Zarattini S. , Aguerri J. A. L. , Biviano A. , Girardi M. , Corsini E. M. , D’Onghia E. 
Published: August 2019

Aims. We aim to study how the velocity segregation and the radial profile of the velocity dispersion depend on the prominence of the brightest cluster galaxies (BCGs).
Methods. We divided a sample of 102 clusters and groups of galaxies into four bins of magnitude gap between the two brightest cluster members. We then computed the velocity segregation in bins of absolute and relative magnitude. Moreover, for each bin of magnitude gap we computed the radial profile of the velocity dispersion.
Results. When using absolute magnitudes, the segregation in velocity is limited to the two brightest bins and no significant difference is found for different magnitude gaps. However, when we use relative magnitudes, a trend appears in the brightest bin: the larger the magnitude gap, the larger the velocity segregation. We also show that this trend is mainly due to the presence, in the brightest bin, of satellite galaxies in systems with small magnitude gaps: in fact, if we study central galaxies and satellites separately, this trend is mitigated and central galaxies are more segregated than satellites for any magnitude gap. A similar result is found in the radial velocity dispersion profiles: a trend is visible in central regions (where the BCGs dominate) but, if we analyse the profile using satellites alone, the trend disappears. In the latter case, the shape of the velocity dispersion profile in the centre of the systems with different magnitude gaps shows three types of behaviour: systems with the smallest magnitude gaps have an almost flat profile from the centre to the external regions; systems with the largest magnitude gaps show a monothonical growth from the low values of the central part to the flat ones in the external regions; and finally, systems with 1.0 <  Δm12 ≤ 1.5 show a profile that peaks in the centre and then decreases towards the external regions.
Conclusions. We suggest that two mechanisms could be responsible for the observed differences in the velocity segregation of the BCGs: an earlier formation of systems with a larger magnitude gap or a more centrally concentrated halo. However, the radial profiles of the velocity dispersion confirm that central galaxies are more relaxed, but that the satellite galaxies do not seem to be affected by the magnitude gap.
Colour-dependent accurate modelling of dynamical parallaxes and masses of visual binaries
Authors: Andrade Manuel 
Published: August 2019

Context. Two fundamental characteristics of binary systems are the masses of their components and their distance to the Earth. In this way, the dynamical parallax is an accurate and very helpful tool. Nevertheless, there has been some concern with regard to the use of a unique linear mass–luminosity relation (MLR) for the entire main sequence (MS).
Aims. This article describes the accurate computation of both dynamical parallaxes and individual masses of visual binaries. The main aim is to formulate a model which would be suitable for binary systems attending to the exact locations of the components on the MS in the HR diagram.
Methods. An analytical model was developed which allows calculation of dynamical parallaxes and individual masses using a non-linear MLR valid for the entire MS. This up-to-date MLR is given by a polynomial of degree 20. In contrast to previous approaches, this model can be applied even in the case of components with unequal masses, that is, with an arbitrarily large difference of magnitudes between them. On the other hand, considering the fundamental equation of the theory that forms the basis of the model, we propose to estimate uncertainties in parallax and masses using Monte Carlo simulations.
Results. The model was validated by means of numerical tests using a synthetic sample comprising 103 systems. The results are much more accurate than those for previous models reported in the literature for deriving dynamical parallaxes and masses. Furthermore, we present dynamical parallaxes and individual masses for the 19 double-lined spectro-interferometric systems with definitive visual orbits and compare the former with the orbital parallaxes as well as with those measured by HIPPARCOS and Gaia. It is worth mentioning that the latter can only be a reliable source when the orbital motion is taken into account. Thus, at present, many Gaia DR2 parallaxes of binaries are biased.
Conclusions. Our model, composed of an exact analytical theory, along with a non-linear MLR, guarantees high accuracy even in cases where the components are of unequal mass.
Pancreatic sphincterotomy improves pain symptoms due to branch-duct intrapapillary mucinous neoplasia without worrisome features: a multicenter study
Authors: Gonzalez Jean-Michel , Lorenzo Diane , Ratone Jean-Philippe , Culetto Adrian , Maire Frédérique , Levy Philippe , Giovannini Marc , Barthet Marc 
Published: August 2019

Abstract
Introduction Branch duct intraductal papillary mucinous neoplasms (BD-IPMNs) require follow-up to detect worrisome features (WF). Data are missing about endoscopic pancreatic sphincterotomy (PS) for symptomatic IPMN.
Patients and methods This was a retrospective multicenter study in four expert centers. Patients treated with endoscopic PS for symptomatic (painful) BD-IPMN without WF were included. Age, sex, follow-up time, characteristics of IPMNs and endoscopic retrograde cholangiopancreatographies (ERCPs), and indications for surgery were recorded.
Results In total, 21 patients were included (median age 68 years, range 45 – 87 years). The median number of cysts was 2 (range 1 – 10), located in the head (59 %), body/tail (17 %), or multifocal (24 %). ERCP including PS was completed in all of the cases, with biliary sphincterotomy in 33 %. Clinical efficacy after one session was 81 % (17/21). Among the failures, one had a second successful PS and three were operated. The final efficacy was 86 % (18/21). Seven patients were operated after a mean of 19 months: four for WF, three for pain. The histopathology showed four low grade dysplasia, one high grade dysplasia, and two no dysplasia. No adenocarcinoma occurred during a follow-up of 99 months (range 14 – 276 months).
Conclusions Endoscopic PS for symptomatic IPMN without WF is effective in more than 80 % of cases, without increasing the risk for adenocarcinoma.
Bedürfnisse nach Information und Unterstützung bei
Krebspatienten: ein Kohortenvergleich von Langzeitüberlebenden
fünf und zehn Jahre nach einer Krebsdiagnose
Authors: Lehmann-Laue Antje , Ernst Jochen , Mehnert Anja , Taubenheim Sabine , Lordick Florian , Götze Heide 
Published: August 2019

Zusammenfassung
Hintergrund Die Berücksichtigung ihrer Informations- und
Unterstützungsbedürfnisse kann langzeitüberlebenden
Krebspatienten helfen, Langzeit- und/oder Spätfolgen ihrer
Erkrankung zu bewältigen. Die Studie untersucht, wie
langzeitüberlebende Krebspatienten die im Krankheitsverlauf erhaltenen
Informationen bewerten und welche Unterstützungsbedürfnisse sie
angeben.
Methode Die Daten wurden in einer querschnittlich angelegten
krebsregisterbasierten Kohortenstudie an 1002 Patienten erhoben (Teilnehmerquote
53%, 5 Jahre nach Diagnose n=660 (65,9%), 10 Jahre nach
Diagnose n=342 (34,1%), Altersdurchschnitt 66,7 Jahre,
männlich 52,9%, Prostatakrebs 25,5%). Als
Befragungsinstrumente wurden der SCNS-SF34-G, der PHQ-9, GAD-7, das
Distress-Thermometer (DT) sowie ein Fragebogen zur Erfassung von
Komorbiditäten eingesetzt. Die Auswertung erfolgte mittels deskriptiv-
und inferenzstatistischer Verfahren.
Ergebnisse Die Informationen zu Behandlungsentscheidungen wurden als am
hilfreichsten, Informationen zur Suche nach Unterstützungsangeboten als
am wenigsten hilfreich wahrgenommen. Jeder fünfte Patient
(19,6%) äußerte den Wunsch nach weiteren Informationen.
Die Mehrzahl der Patienten (81,3%) gab mindestens ein
Unterstützungsbedürfnis an, im Mittel sind es 11
(SD=7,14) unerfüllte Unterstützungsbedürfnisse.
Am häufigsten beziehen sich diese auf Ängste vor dem
Wiederauftreten/Fortschreiten der Erkrankung, auf körperliche
Probleme sowie auf den Wunsch nach einem festen Ansprechpartner für
medizinische Fragen. Eine höhere körperliche
Komorbidität und Distress geht mit einer erhöhten
Wahrscheinlichkeit für ein Unterstützungsbedürfnis
einher (p<0,001).
Schlussfolgerungen Es sind Versorgungs- und Nachsorgemodelle
nötig, die mit einem multidisziplinären Ansatz medizinische
Aspekte und psychosoziale Bedürfnisse berücksichtigen.
Validierung der deutschsprachigen Version der Multidimensional
Offline and Online Peer Victimization Scale (MOOPV) für Jugendliche an
einer Schülerstichprobe
Authors: Haid-Stecher Nina , Exenberger Silvia , Unterrainer Christine , Bliem Harald R. , Sevecke Kathrin , Juen Barbara 
Published: August 2019

ZusammenfassungPeer Viktimisierung (PV) im Jugendalter kann mit psychischen Belastungen
über die Lebensspanne hinweg einhergehen. Obwohl eine Vielzahl von
Instrumenten zur Erfassung von PV entwickelt wurden, fehlen häufig
Evidenzen für deren Einsatz. Besonders bei den Instrumenten zur
Erfassung von Cybermobbing fehlen oft psychometrische Berechnungen, wie
Faktorenanalysen zur Untersuchung der angenommenen Skalen und Angaben zu
Validität und Reliabilität. Die Multidimensional Offline and
Online Peer Victimization Scale (MOOPV) von Sumter und Kollegen stellt eine
Ausnahme dar, ist aber bisher nur auf Niederländisch verfügbar.
Dieser Fragebogen ist nicht mediumspezifisch ausgelegt, hinreichend
psychometrisch geprüft und unterschiedet zwischen verschiedenen Formen
von direkter und indirekter, online und offline PV. Ziel dieser Studie ist die
Präsentation und psychometrische Überprüfung der
deutschsprachigen Version der Multidimensional Offline and Online Peer
Victimization Scale (MOOPV). Die Faktorenstruktur, Validität und
Reliabilität wurde anhand der Daten von 777 SchülerInnen
zwischen 10 und 20 Jahren untersucht. Eine konfirmatorische Faktorenanalyse
stützte das Vier-Faktorenmodell. Die Skalen direkte offline, indirekte
offline, direkte online und indirekte online PV weisen gute interne Konsistenzen
sowie Konstruktvalidität auf. Somit ist der MOOPV für die
Erfassung von PV im Jugendalter für Forschung und Praxis geeignet.
Ist eine Hemmung der röntgenologischen Progression bei axialer Spondyloarthritis möglich?
Authors: Poddubnyy Denis , Sieper Joachim 
Published: August 2019

ZusammenfassungStrukturschäden bei der axialen Spondyloarthritis (axSpA) entstehen in der Regel zunächst in den Sakroiliakalgelenken und später in der Wirbelsäule. Während die Entwicklung von Strukturschäden an den Sakroiliakalgelenken v. a für die Diagnose und Klassifizierung relevant ist (nicht-röntgenologsiche vs. röntgenologische axSpA), haben Strukturschäden an der Wirbelsäule, die v. a auf den Prozess der Knochenneubildung (Entwicklung von Syndesmophyten mit knöcherner Ankylose) zurückzuführen sind – eine klare Relevanz für die langfristige Prognose bei der Erkrankung. Strukturschäden bei der axSpA werden in der Regel auf konventionellen Röntgenaufnahmen des Achsenskeletts beurteilt und daher häufig als röntgenologische Progression bezeichnet. In dieser Übersichtsarbeit werden die Möglichkeiten der Prävention und/oder Verzögerung der röntgenologischen Progression bei Patienten mit axSpA diskutiert.
Resolution of Brainstem Edema after Neurosurgical Occlusion of Dural Arteriovenous Fistulas of the Craniocervical Junction: Report of Three Cases and Review
Authors: Firsching Raimund , Kohl Jana , Skalej Martin , Beuing Oliver 
Published: August 2019

Abstract
Objective Dural arteriovenous fistulas of the craniocervical junction are rare but the most dangerous of spinal fistulas. The concurrent brainstem edema has been attributed to increased pressure within the venous outflow of the brainstem and upper cord, but the differential diagnosis of brainstem edema is demanding. This report presents the details of clinical findings, magnetic resonance imaging (MRI) , surgical techniques, and follow-up of these fistulas.
Methods Three cases with dural arteriovenous fistulas and edema of the brainstem and upper cord are described plus a review of the literature. Clinical presentation, radiologic findings, and a follow-up of outcomes are presented.
Results All three cases with misleading diagnoses at the time of referral experienced variable degrees of tetraparesis with brainstem edema on MRI. Congested veins on MRI raised the suspicion of a vascular origin of the edema. The diagnosis was established with highly specific angiography with microcatheters identifying a feeder from the ascending pharyngeal artery in two cases and a tentorial feeder from the carotid artery in one case. The operation included temporary clipping of the fistula and neuromonitoring. Follow-up confirmed improvement of clinical signs and resolution of the brainstem edema on MRI.
Conclusion Edema of the brainstem and cervical cord may be caused by a dural arteriovenous fistula of the craniocervical junction. Surgical occlusion may be an efficient option for the complete resolution of radiologic and clinical symptoms.