bluuebunny/crossref_metadata_2025_split huggingface dataset:
R2R3‐MYB transcription factor MYB6 promotes anthocyanin and proanthocyanidin biosynthesis but inhibits secondary cell wall formation in Populus tomentosa
Authors: Wang Lijun , Lu Wanxiang , Ran Lingyu , Dou Liwen , Yao Shu , Hu Jian , Fan Di , Li Chaofeng , Luo Keming 
Published: April 2019

SummaryThe secondary cell wall is an important carbon sink in higher plants and its biosynthesis requires coordination of metabolic fluxes in the phenylpropanoid pathway. In Arabidopsis (Arabidopsis thaliana), MYB75 and the KNOX transcription factor KNAT7 form functional complexes to regulate secondary cell wall formation in the inflorescence stem. However, the molecular mechanism by which these transcription factors control different branches of the phenylpropanoid pathway remains poorly understood in woody species. We isolated an R2R3‐MYB transcription factor MYB6 from Populus tomentosa and determined that it was expressed predominately in young leaves. Overexpression of MYB6 in transgenic poplar upregulated flavonoid biosynthetic gene expression, resulting in significantly increased accumulation of anthocyanin and proanthocyanidins. MYB6‐overexpression plants showed reduced secondary cell wall deposition, accompanied by repressed expression of secondary cell wall biosynthetic genes. We further showed that MYB6 interacted physically with KNAT7 and formed functional complexes that acted to repress secondary cell wall development in poplar and Arabidopsis. The results provide an insight into the transcriptional mechanisms involved in the regulation of the metabolic fluxes between the flavonoid and lignin biosynthetic pathways in poplar.
Effects of Multiple Sprint Test on Average Speed and Exercise Heart Rate in Amateur Soccer Players
Authors: Ece Cuma , Şen Murat , Çetin Semra , Aydoğan Alaeddin , Çetin H. Nedim 
Published: April 2019

The purpose of this study is to investigate the effects of multiple sprints on average speed and exercise heart rate of U18 female and U18 male amateur soccer players. In this study, totally 42 amateur soccer players including U18 (n= 21) and U18 (n=21) players performed 7 repetitive sprints of 34.2 meters with a 25 seconds jog of 50 meters distance. During exercise and recovery period, heart rate per minute was recorded by Polar Heart Rate Telemetry. In statistical analyzes, t-tests were used in two groups comparisons, while the effects of other variables on average speed and cardiovascular parameters were determined by two way analyzes of variance.Results of this study, U18 male players’ average speed of 5.14 m/ sec is higher than the U18 female players’ average speed of 4.62 m/sec. The physical characteristics of U18 male and U18 female amateur soccer players were different in favors of the U18 male players, and that gender was significantly effective on the average speed rather than the number of sprints. Heart rate per minute has continually increased in different characteristics at the start, finish and recovery periods of the sprints until the finish of the seventh sprint in both amateur U18 male and U18 female. It found increase in the Sprint time were as follows; U18 female 0.40 sec and U18 male 0.20 second. Decrease in the average speed was U18 female 0.28 sec., and U18 male 0.15 second.Conclusion, in multiple speed tests, maximum and average speed of the U18 amateur female and male soccer players, the decrease rate in their speed and their cardiovascular responses show differences. In U18 Amateur, males seem to be faster and have the ability of keeping speed at a high level, and the ability of intensity and recovery in maximal level in a short time than the females. In the Bangsbo test, the recovery time in men should be less than 25 seconds.
The impact of patient navigation: a scoping review protocol
Authors: Reid Amy E. , Doucet Shelley , Luke Alison , Azar Rima , Horsman Amanda Rose 
Published: April 2019

Review objective/question:
The objective of this review is to synthesize the evidence on the impact of patient navigation for all populations across all settings. The question of this review is: What is the existing evidence on the impact of patient navigation?
Reduced insulin sensitivity in young, normoglycaemic subjects alters microvascular tissue oxygenation during postocclusive reactive hyperaemia
Authors: Townsend Dana K. , Deysher Daniel M. , Wu Esther E. , Barstow Thomas J. 
Published: April 2019

New Findings
What is the central question of the study?
Are measures of reduced insulin sensitivity in young, normoglycaemic subjects correlated with near‐infrared spectroscopy‐derived microvascular responsiveness [tissue oxygen saturation reperfusion rate (STO2 upslope)] during postocclusive reactive hyperaemia?

What is the main finding and its importance?
A sevenfold range of hepatic insulin sensitivity is significantly correlated (r = 0.44, P = 0.02) with STO2 upslope after transient tissue ischaemia. Near‐infrared spectroscopy may be an important tool for determining altered microvascular function before onset of hyperglycaemia. Identification of pre‐type 2 diabetes much earlier than with the present clinical criteria is important for pre‐emptive measures against microvascular deterioration.

AbstractNear‐infrared spectroscopy (NIRS) measurement of postocclusive reactive hyperaemia (PORH) tissue oxygen saturation reperfusion rate [STO2 upslope (as a percentage per minute)] has recently been correlated with the percentage of flow‐mediated dilatation (%FMD). Cardiovascular disease is associated with impairments in %FMD. Reduced insulin sensitivity may negatively affect the vascular system for many years before prediabetes/type 2 diabetes states. The aim of this study was to determine whether static and dynamic STO2 parameters during PORH are correlated with reduced insulin sensitivity in young, normoglycaemic subjects. Glucose and insulin were measured during an oral glucose tolerance test in 18‐ to 26‐year‐old, healthy subjects (11 men and 11 women), and STO2 was measured during PORH of antebrachial muscle. Hepatic (ISIHOMA) and whole‐body (ISICOMP) insulin sensitivities were calculated. The STO2 upslope was negatively correlated with minimal STO2 (r = −0.5, P = 0.01). The change of STO2 from minimum to baseline (ΔSTO2) was significantly negatively correlated with fasting insulin (r = −0.5, P = 0.01) and a positively correlated with ISIHOMA (r = 0.65, P = 0.001). The minimum STO2 was significantly negatively correlated with ISIHOMA, and STO2 upslope was significantly positively correlated with ISIHOMA (r = 0.44, P = 0.02). The minimum STO2 (a measure of O2 extraction while the cuff was inflated), ΔSTO2 (a measure of the amount of reperfusion) and STO2 upslope (a measure of responsiveness of the microcirculation to ischaemia) were all positively correlated with ISIHOMA, one of the longest‐used measures of insulin sensitivity. The NIRS‐derived STO2 might be a useful tool for assessing how levels of reduced insulin sensitivity in young, normoglycaemic adults affect the microvasculature.
Altered retinoid metabolism gene expression in chronic Stevens-Johnson syndrome
Authors: Srividya Gurumurthy , Angayarkanni Narayanasamy , Iyer Geetha , Srinivasan Bhaskar , Agarwal Shweta 
Published: April 2019

BackgroundStevens-Johnson syndrome (SJS), a blistering disorder of the skin and mucous membrane, leads to ocular morbidity in >60% of cases. Retinoids are vital micronutrients for vision, regulating corneal and conjunctival cell proliferation, differentiation and immune function. This prospective case–control study probed for alterations in retinoid metabolism by evaluating retinoic acid receptor signalling in the conjunctival cells of patients with SJS.MethodsImprints were collected from the bulbar conjunctiva of patients with chronic SJS. The gene expression of retinoic acid receptors, namely, RXRA, RARA, RARG, RORA; the fibrosis marker TGFβ and its receptor TGFβRII; the transcription factors PPAR-γ, STRA6 and Stat3; the enzymes aldehyde dehydrogenase (ALDH1a1), alpha-1 antitrypsin (A1AT); and the Cyp genes Cyp26a1 and Cyp26b1 were assessed by quantitative PCR in patients with SJS pre-mucous (n = 34) and post-mucous membrane graft (MMG) intervention (n=19) in comparison with age-matched/sex-matched healthy controls (n=20). Western blot analysis of ALDH1a1, RARA and RARG were done in the conjunctival imprint cells.ResultsThe transcript levels of ALDH1a1, RXRA, RORA, STRA6, Cyp26a1 and Cyp26b1 were decreased around 4, 26, 17, 129, 9 and 8 folds, respectively, and RARA, RARG, PPAR-γ, TGFβ, TGFβRII were increased by 12, 15, 51, 16 and 87 folds, respectively, in SJS conjunctiva at the pre-MMG stage. The changes in RORA, Cyp26a1, Cyp26b1, RARA and Stat3 were statistically significant (p<0.05). Changes in protein expression of ALDH1a1, RARA and RARG supported the gene expression changes.ConclusionsThe study provides the first experimental insight into the role of retinoid metabolism in the ocular sequelae of chronic SJS.
Choroidal amelanotic tumours: clinical differentiation of benign from malignant lesions in 5586 cases
Authors: Welch R Joel , Newman Jennifer H , Honig Stephanie E , Mayro Eileen L , McGarrey Mark , Graf Alexander E , Selzer Evan B , Acaba-Berrocal Luis A , Considine Sean P , Malik Kunal , Shields Jerry A , Shields Carol L 
Published: April 2019

PurposeTo investigate demographics and clinical features of patients with amelanotic choroidal tumours.DesignRetrospective analysis.MethodsComparison of demographic and clinical features of various amelanotic choroidal tumours based on stratification by patient age, sex and tumour diameter. Included were all patients with amelanotic choroidal tumours evaluated on the Ocular Oncology Service, Wills Eye Hospital, Philadelphia, Pennsylvania, USA, over a 45-year time period.ResultsA total of 5586 amelanotic choroidal tumours in 4638 eyes of 4441 patients were included with a mean age at presentation of 58 years (median 60, range 0.1–100 years). Most patients were white (95%), female (56%) and with unilateral lesion (96%). By comparison, amelanotic melanoma presented at a younger mean age (57 years) compared with metastasis (60 years, p<0.001), nevus (61 years, p<0.001), lymphoma (65 years, p<0.001), sclerochoroidal calcification (70 years, p<0.001) and peripheral exudative haemorrhagic chorioretinopathy (80 years, p<0.001). Melanoma presented at an older mean age compared with osteoma (30 years, p<0.001), granuloma (42 years, p<0.001), haemangioma (49 years, p<0.001) and inflammatory choroidal lesions (49 years, p<0.001). Differences in race and sex were also seen between the various amelanotic choroidal lesions. With few exceptions, amelanotic melanoma had significantly larger basal diameter, greater thickness, more frequent association with subretinal fluid and more often ultrasonographically hollow, compared with other amelanotic choroidal lesions.ConclusionUnderstanding the demographic and clinical features of amelanotic choroidal melanoma and other amelanotic lesions could lead to an earlier and more accurate diagnosis.
Association between focal lamina cribrosa defects and optic disc haemorrhage in glaucoma
Authors: Mistry Vijay , An Dong , Barry Christopher J , House Philip H , Morgan William H 
Published: April 2019

Background/aimsTo explore the relationship between focal lamina defect (LD) size and optic disc haemorrhages (DH) in glaucomatous eyes.MethodsRadial B-scan images at 15° intervals obtained using enhanced depth imaging (EDI) spectral-domain optical coherence tomography (OCT) were performed on a group of subjects previously assessed for DH every 3 months over a period of 5 years. EDI-OCT scans were assessed for the presence of focal lamina cribrosa defects by a single observer.Results119 eyes from 62 subjects (44 females, 18 males) were analysed. 44 eyes (37%) were noted to have at least 1 LD, and of those, eight eyes had more than one defect. 68 eyes (57%) were observed to have at least one DH occur over the course of monitoring. 48 eyes (40%) had recurrent DH, with a mean of 5.17 haemorrhages over the 5-year period. Type 1 focal LD (p=0.0000, OR 7.17), glaucoma progression (p=0.0024, OR 0.32) and ArtDiff (p=0.0466, OR 1.04) were significantly associated as predictors of DH. No correlation between the size of the LD and DH occurrence (p=0.6449, Spearman rank correlation) was found.ConclusionFocal lamina cribrosa hole-type defects were significantly associated with an increase in DH occurrence over the preceding 5 years. The lack of association between defect size and DH suggests that DH and lamina defects may have separate links to the glaucomatous process.
Identifying the lifetime cognitive and socioeconomic antecedents of cognitive state: seven decades of follow-up in a British birth cohort study
Authors: Richards M , James Sarah-Naomi , Sizer Alison , Sharma Nikhil , Rawle Mark , Davis Daniel H J , Kuh Diana 
Published: April 2019

ObjectivesThe life course determinants of midlife and later life cognitive function have been studied using longitudinal population-based cohort data, but far less is known about whether the pattern of these pathways is similar or distinct for clinically relevant cognitive state. We investigated this for Addenbrooke’s Cognitive Examination third edition (ACE-III), used in clinical settings to screen for cognitive impairment and dementia.DesignLongitudinal birth cohort study.SettingResidential addresses in England, Wales and Scotland.Participants1762 community-dwelling men and women of European heritage, enrolled since birth in the Medical Research Council (MRC) National Survey of Health and Development (the British 1946 birth cohort).Primary outcomeACE-III.ResultsPath modelling estimated direct and indirect associations between apolipoprotein E (APOE) status, father’s social class, childhood cognition, education, midlife occupational complexity, midlife verbal ability (National Adult Reading Test; NART), and the total ACE-III score. Controlling for sex, there was a direct negative association betweenAPOEε4 and the ACE-III score (β=−0.04 [–0.08 to –0.002], p=0.04), but not betweenAPOEε4 and childhood cognition (β=0.03 [–0.006 to 0.069], p=0.10) or the NART (β=0.0005 [–0.03 to 0.03], p=0.97). The strongest influences on the ACE-III were from childhood cognition (β=0.20 [0.14 to 0.26], p<0.001) and the NART (β=0.35 [0.29 to 0.41], p<0.001); educational attainment and occupational complexity were modestly and independently associated with the ACE-III (β=0.08 [0.03 to 0.14], p=0.002 and β=0.05 [0.01 to 0.10], p=0.02, respectively).ConclusionsThe ACE-III in the general population shows a pattern of life course antecedents that is similar to neuropsychological measures of cognitive function, and may be used to represent normal cognitive ageing as well as a screen for cognitive impairment and dementia.
Health-related quality of life and prevalence of six chronic diseases in homeless and housed people: a cross-sectional study in London and Birmingham, England
Authors: Lewer Dan , Aldridge Robert W , Menezes Dee , Sawyer Clare , Zaninotto Paola , Dedicoat Martin , Ahmed Imtiaz , Luchenski Serena , Hayward Andrew , Story Alistair 
Published: April 2019

ObjectivesTo compare health-related quality of life and prevalence of chronic diseases in housed and homeless populations.DesignCross-sectional survey with an age-matched and sex-matched housed comparison group.SettingHostels, day centres and soup runs in London and Birmingham, England.ParticipantsHomeless participants were either sleeping rough or living in hostels and had a history of sleeping rough. The comparison group was drawn from the Health Survey for England. The study included 1336 homeless and 13 360 housed participants.Outcome measuresChronic diseases were self-reported asthma, chronic obstructive pulmonary disease (COPD), epilepsy, heart problems, stroke and diabetes. Health-related quality of life was measured using EQ-5D-3L.ResultsHoused participants in more deprived neighbourhoods were more likely to report disease. Homeless participants were substantially more likely than housed participants in the most deprived quintile to report all diseases except diabetes (which had similar prevalence in homeless participants and the most deprived housed group). For example, the prevalence of chronic obstructive pulmonary disease was 1.1% (95% CI 0.7% to 1.6%) in the least deprived housed quintile; 2.0% (95% CI 1.5% to 2.6%) in the most deprived housed quintile; and 14.0% (95% CI 12.2% to 16.0%) in the homeless group. Social gradients were also seen for problems in each EQ-5D-3L domain in the housed population, but homeless participants had similar likelihood of reporting problems as the most deprived housed group. The exception was problems related to anxiety, which were substantially more common in homeless people than any of the housed groups.ConclusionsWhile differences in health between housed socioeconomic groups can be described as a ‘slope’, differences in health between housed and homeless people are better understood as a ‘cliff’.
Parent and family perspectives on home-based newborn care practices in lower-income countries: a systematic review of qualitative studies
Authors: Bazzano Alessandra N , Felker-Kantor Erica , Eragoda Shalini , Kaji Aiko , Horlick Raquel 
Published: April 2019

ObjectivesTo understand family and parent perspectives on newborn care provided at home to infants in the first 28 days of life, in order to inform behavioural interventions for improving care in low-income countries, where the majority of newborn deaths occur.DesignA comprehensive, qualitative systematic review was conducted. MEDLINE/PubMed, Embase and Cumulative Index of Nursing and Allied Health databases were systematically searched for studies examining the views of parents and family members on newborn care at home. The search period included all studies published from 2006 to 2017. Studies using qualitative approaches or mixed-methods studies with substantial use of qualitative techniques in both the methods and analysis sections were included. Studies meeting the inclusion criteria were extracted and evaluated using Critical Appraisal Skills Programme guidelines. Following the initial selection and appraisal, barriers and facilitators to recommended care practices across several domains were synthesised.ResultsOf 411 results retrieved, 37 met both inclusion and quality appraisal criteria for methodology and reporting. Geographical representation largely reflected that of newborn health outcomes globally, with the majority of studies conducted in the region of Sub-Saharan Africa and South Asia. Specific barriers and facilitators were identified among a range of domains including: cord care, drying and wrapping, thermal control, skin to skin contact, hygiene, breast feeding, care-seeking for illness, and low birthweight recognition. Cross cutting facilitators, common to all domains were evident and includeddelivery at a health facility, inclusion of female relatives in care counselling, lower healthcare costs, and exposure to newborn care behaviour change messaging in the community.ConclusionsWhen designing behavioural interventions to address newborn mortality at scale, policy-makers and practitioners must include barriers and facilitators important to families in low-income settings.PROSPERO registration numberCRD42016035674.
A double blind, randomised, placebo-controlled trial to evaluate the efficacy of metformin to treat preterm pre-eclampsia (PI2 Trial): study protocol
Authors: Cluver Catherine , Walker Susan P , Mol Ben W , Hall David , Hiscock Richard , Brownfoot Fiona C , Kaitu’u-Lino Tu’uhevaha J , Tong Stephen 
Published: April 2019

IntroductionPre-eclampsia is a major complication of pregnancy, globally responsible for 60 000 maternal deaths per year, and far more fetal losses. There is no definitive treatment other than delivery. A therapeutic that could quench the disease process would be useful to treat preterm pre-eclampsia, as it could allow these pregnancies to safely continue to a gestation where fetal outcomes are significantly improved. We have published preclinical data to show that metformin, a drug known to be safe in pregnancy and commonly used to treat gestational diabetes, has potent biological effects making it another promising candidate to treat pre-eclampsia. Here, we describe a phase II clinical trial to examine whether administering extended-release metformin may be effective in treating women with preterm pre-eclampsia (PI2 Trial).MethodsThe PI2 Trial is a phase II, double blind, randomised controlled trial that aims to recruit 150 women with preterm pre-eclampsia (gestational age 26+0 to 31+6 weeks) who are being managed expectantly. Participants will be randomised to receive either 3 g of metformin or placebo daily. The primary outcome is time from randomisation until delivery. A delay in delivery of 5 days is assumed to be clinically relevant. The secondary outcomes will be a maternal composite and neonatal composite outcome. All other outcomes will be exploratory. We will record adverse events.Ethics and disseminationThis study has ethical approval (Protocol number M16/09/037 Federal Wide Assurance Number 00001372, Institutional Review Board Number IRB0005239), is registered with the Pan African Clinical Trial Registry (PACTR201608001752102) and the South African Medicine Control Council (20170322). Data will be presented at international conferences and published in peer-reviewed journals.Trial registration numberPACTR201608001752102; Pre-results.
Measuring diabetes guideline adherence with claims data: systematic construction of indicators and related challenges
Authors: Ulyte Agne , Bähler Caroline , Schwenkglenks Matthias , von Wyl Viktor , Gruebner Oliver , Wei Wenjia , Blozik Eva , Brüngger Beat , Dressel Holger 
Published: April 2019

ObjectivesIndicators of guideline adherence are frequently used to examine the appropriateness of healthcare services. Only some potential indicators are actually usable for research with routine administrative claims data, potentially leading to a biased selection of research questions. This study aimed at developing a systematic approach to extract potential indicators from clinical practice guidelines (CPG), evaluate their feasibility for research with claims data and assess how the extracted set reflected different types of healthcare services. Diabetes mellitus (DM), Swiss national guidelines and health insurance claims data were analysed as a model case.MethodsCPG for diabetes patients were retrieved from the Swiss Endocrinology and Diabetes Society website. Recommendation statements involving a specific healthcare intervention for a defined patient population were translated into indicators of guideline adherence. Indicators were classified according to disease stage and healthcare service type. We assessed for all indicators whether they could be analysed with Swiss mandatory health insurance administrative claims data.ResultsA total of 93 indicators were derived from 15 CPG, representing all sectors of diabetes care. For 63 indicators, the target population could not be identified using claims data only. For 67 indicators, the intervention could not be identified. Nine (10%) of all indicators were feasible for research with claims data (three addressed gestational diabetes and screening, five screening for complications and one glucose measurement). Some types of healthcare services, eg, management of risk factors, treatment of the disease and secondary prevention, lacked corresponding indicators feasible for research.ConclusionsOur systematic approach could identify a number of indicators of healthcare service utilisation, feasible for DM research with Swiss claims data. Some areas of healthcare were covered less well. The approach could be applied to other diseases and countries, helping to identify the potential bias in the selection of indicators and optimise research.