bluuebunny/crossref_metadata_2025_split huggingface dataset:
PO-098 Effect of HIIT on mitochondrial telomerase of skeletal muscle in aged rats
Authors: Liu Yiyi , Su Hao , Jiang Zhongye , Wen Tianhao , Shao Jia 
Published: October 2018

Objective The HIIT and moderate-intensity exercise are two different exercise models among the public fitness. In recent years, HIIT become more and more popular, unfortunately, there is a tremendous lack of research being done effects of mitochondrial reverse transcriptase (TERT) on age-related degeneration of skeletal muscle by HIIT. The purpose of this study was to compare the HIIT group and moderate-intensity group, and research difference of telomerase expression and cardiopulmonary endurance between the exercise group and the quiet control group was discussed.
Methods fifty-nine male Wistar rats were divided into three groups at random: control group (Q=19), moderate-intensity intervention group (M=20), and HIIT intervention group (H=20). The rats in Q group did not any exercise, and the rats in M group developed the exercise with 60% VO2max intensity for 8 weeks. H group did a training program for an 8-week exercise with alternating 40%, 60%, and 80% VO2max intensities. The rats in the experimental group were exercised for 50 minutes every day and trained for 5 days per week. After the baseline value group was sampled, each group of rats was selected after the training reached the specified number of weeks (4 and 8 weeks), and the maximum oxygen uptake test was performed before the material was taken. Single factor analysis of variance were used to assess differences in VO2max, and expression of protein between conditions.
Results It was found that H group VO2max was significantly higher than M group and Q group (P<0.05). At same time, the mTERT expression of the M group at the 4th week was significantly higher than that of the Q group (P<0.05). The mTERT expression in group H was significantly higher than that in group Q at week 8 (P<0.05).There was no significant difference between the H group and the Q group at 8th week (P<0.05).
Conclusions 1. HIIT exercise lasting for 8 weeks can effectively inhibit the decrease of maximal oxygen uptake in aging rats compared with moderate exercise. 2. HIIT training for 8 weeks promotes the expression of mTERT; 3. The maintenance of VO2max in aging rats may be related to the enhancement of mitochondrial antioxidant function by HIIT-promoted TERT to mitochondrial translocation.
PO-018 A Follow-up Study of 8-10 age pupil's physical activity status and related factor
Authors: Sun Qianli , Ren Hong 
Published: October 2018

Objective Regular physical activity in children has many benefits，including reducing the occurrence of obesity and lower levels of cardiovascular disease risk factors, promote bone mass deposition, improve health. Our research in order to using accelerometer survey children daily PA status in our country, and analyzes its characteristic.
Methods Our research assessed the PA of the same group of children in Beijing yuxin Primary school for three consecutive years using ActiGraph accelerometers. The total available valid data are 183 children (male 94, female 89).
Results (1)The average total PA is 627.2±134.5 counts/min（boy accrued 665.6 ± 139.1 counts/min and girl 579.8 ± 112.2 counts/min ）, among them boy accrued 59.0min/d and girl 48.1min/d of MVPA. 35% of participants could meet the WHO PA guideline (boys =49%, girls = 18%).
(2) MVPA increased significantly from 8 to 9 years of age（MVPA of 8year-old children:53.2min/d,9year-old children:56.1min/d）and MVPA decline slightly from 9 to 10 (10 year-old children:55.8min/d).And both boys and girls meet this regular.
(3) Both in weekdays and weekend, the MVPA time of boys (60.2min/d,57.8min/d) are outstandingly higher than girls ( 49.7min/d ,46.5min/d).
(4) TWO-way (gender *BMI category) ANOVA revealed that girls were less active than boys.
(5) The moderate and vigorous physical activity time of obese children in weekends are 42.3 min/d while those of normal children are 49.7 min/d, (p<0.05).
(6) The total PA level, VPA, MVPA are negatively correlated with body fat percentage while LPA is positively correlated with BMI.
Conclusions Objective data indicated that Chinese youth are generally not physically active enough, and lower levels of PA were observed in girls, and during weekend days. The findings of this study will better inform the development of PA-related policies in schools, the design of PA intervention programs.
PO-024 Associations between the Sedentary Behavior and Cardiovascular Risk Factors in Adults of 40-49year
Authors: Lin Jiashi , Yang Xiaoyue , Yan Yi 
Published: October 2018

Objective Large amounts of pieces of evidence suggest sedentary behavior(SB) might have a negative impact on all-cause mortality, cardiovascular disease, and metabolic system. Our aim is to explore the associations between sedentary behavior and cardiovascular risk factors in adults of 40-49year.
Methods Participants(N=372) were adults 40-49 years old who had a survey by using adult sit-Q-7d. The indexes of body mass index(BMI) and Waist circumference(WC) were measured as per standard protocols and blood pressure measured using an automated sphygmomanometer (Carescape V100; GE Healthcare, UK) following five minutes seated rest. HDL cholesterol (HDL-C), LDL cholesterol (LDL-C), triglycerides(TG) and fasting plasma glucose(FPG) were assessed for blood sampling of venous blood following an overnight fast (Cholestech LDX; Alere Inc, USA). We analyzed data in 2 h/day and 1 h/day as segmentation criteria of sitting time were classified as 8 groups(0<2, 2-4, 4-6, 6-8, 8-10, >10 h/day) and 10 groups(<1, 1-2, 2-3, 3-4, 4-5, 5-6, 6-7, 7-8, 8-9，>9 h/day). Statistical analyses were performed using STATA software 15.0(STATA Corp, College Station, TX). We performed multiple linear regression models adjusting for health-related confounders to assess the associations between SB of difference range and risk factors of cardiovascular disease.
Results (1)When the sitting time as category criteria in 2 h/day, the relationship of LDL-C(β=0.651，95%CI=0.069 to 1.232, P=0.028) and HDL-C(β=0.129，95%CI=0.013 to 0.245, P=0.028) to sitting time in segment of 6-8h/day were statistically significant after adjustment for relative factor of risk; and the same in TC(β=0.932，95%CI=0.244 to 1.620, P=0.008) to sitting time in segment of 8-10h/day; (2) When the sitting time as category criteria in 1 h/day, in 3-4h/day segment, the index of BMI(β=1.550，95%CI=0.025 to 3.074, P=0.046) had a significant rise; in 7-8h/day segment, the index of LDL-C(β=0.919，95%CI=0.155 to 1.683, P=0.019) also had a significant rise; as well as, in 8-9h/day segment, the index of TC(β=1.531，95%CI=0.497 to 2.565, P=0.004) had a highly significant. (3) As has mentioned above, the threshold for each segment of sitting time for the index of LDL-C in 7-8h/day segment and the index of TC in 8-9h/day.
Conclusions This study suggests that (1) the different category criteria of sitting time, for categories criteria in 1h/d or 2h/d, will help reduce the risk of Cardiovascular risk in some degree and determining to their dose-response relationships. (2) For the 40-49year adult of health, the sitting time threshold is less than 7 h/d, in order to decrease the detrimental effects on metabolic markers associated with cardiovascular disease and metabolic system. (3) As well as the sitting time threshold is less than 3 h/d for adding to the negative aging effect on risk of cardiovascular markers.
PO-034 Effect of Voluntary Exercise on Cartilage Morphology of Knee Osteoarthritis in Obese Mice Induced by High-fat Diet
Authors: Zong Wenhao , Liu Yang , Dai Lulu , Dong Guijun 
Published: October 2018

Objective To examine the effect of voluntary wheel-running exercise on cartilage morphology of knee osteoarthritis(KOA) in obese mice induced by high-fat diet,and explore the protective role of 4 weeks voluntary wheel-running exercise on KOA,finally providing effective experimental evidence for clinical treatment of knee osteoarthritis.
Methods C57BL/6J mice were randomly assigned to the C-Sed group,C-Ex group,HF-Sed group and HF-Ex group.The control groups were fed a control diet(13.5% kcal from fat),and the high-fat groups were fed a high-fat diet(60% kcal from fat).After feeding 8 weeks different diets,the exercise groups were starting running.In order to examine the effect of voluntary wheel-running exercise on cartilage morphology of KOA,the joint of knee were harvested to be fixed,decalcified and embedded in paraffin,and the four-micrometer-thick sections were stained with both HE and toluidine blue .
Results After feeding twelve weeks different diets,the body mass of the high-fat diet group mice has a significant increase,which demonstrates that high-fat diet could successfully induce the mice obese.From the results of HE and toluidine blue,in comparison to the C-Sed group,the surface of the knee articular cartilage in the HF-Sed group was not intact and smooth,and the thickness of articular cartilage has a significant decrease（p<0.001）;contrary to the HF-Sed group,the surface of the knee articular cartilage in HF-Ex group was slightly smooth,and there was significant increase in cartilage thickness.
Conclusions Four weeks voluntary wheel-running exercise can increase cartilage thickness ,decrease the Mankin’s score and delay the degeneration of knee cartilage in obese mice.To conclude,the short-term wheel-running exercise protects against obesity-induced KOA.
PO-121 Influence of HDAC1 inhibitor on the E3-ligases expression in rat soleus during hindlimb unloading
Authors: Belova Svetlana , Mochalova Ekaterina , Shenkman Boris , Nemirovskaya Tatiana 
Published: October 2018

Objective Muscle unloading leads to its atrophy development. The MuRF-1 and MAFbx E3-ligases expression is increasing under this condition. FOXO3 was considered to be the only transcription factor that triggers E3-ligases expression. Beharry A.W. et al pinpoints HDAC1 as a primary regulator of FoxO in skeletal muscle that is both sufficient and required for skeletal muscle atrophy. We aimed to determine the role of histone deacetylase 1 (HDAC1) proteins in activation of MuRF-1 and MAFbx E3-ligases expression at the early stage of muscle unloading.
Methods We investigate it by CI-994 (inhibitor of HDAC1) administration in male Wistar rats (180-200 g) upon 3-day hindlimb suspension. The method of hindlimb suspension was described in Morey-Holton E & Globus R (2002). 24 animals were divided into 3 groups (n=8 in each): C-control, CI - hindlimb suspension with CI-994 (i.p. 1 mg/kg/day), or placebo (HS group) administration. The animals were anaesthetized with an i.p. injection of tribromoethanol (240 mg/kg), soleus muscles were surgically excised, frozen in liquid nitrogen. The Western blot and RT-PCR analyzes were done. The statistical analysis was performed using REST 2009 v.2.0.12 and Bio-Rad CFX Manager programs at the significance level set at 0.05. The significant differences between groups were statistically analyzed using Mann-Whitney test.
Results The evaluation of the levels of mRNA expression of MuRF-1 and MAFbx showed that CI-994 treatment inhibited unloading induced up-regulation of MAFbx in CI group but had no effect on mRNA expression of MuRF-1. After unloading, mRNA expression of MAFbx increased 2.12-fold (p < 0.05) in HS group. There were statistically significant differences in MAFbx mRNA expression between HS and CI groups. When compared with the control, unloading increased MuRF-1 mRNA expression 1.67- and 1.56-fold in HS and CI groups, respectively. CI-994 treatment also inhibited unloading-induced upregulation of mRNA expression of ubiquitin. The levels of ubiquitin mRNA expression when compared with the control were 4.21- and 2.32-fold in HS and CI groups, respectively. We did not find any differences in the content of phosphorylated anabolic signaling system components (Akt/mTOR/S6k) between both suspended groups (CI and HS).
Conclusions Therefore, HDAC1 inhibiting prevented hindlimb suspension-induced up-regulation of MAFbx and ubiquitin, but did not any effect MuRF-1expression.
This work was supported by Russian Science Foundation (grant № 18-15-00062).
PO-127 Research progresses of exercise intervention methods in non- alcoholic fatty liver disease
Authors: Luo Chao , Li Xiangping , Liu Xinhua , Song Lihui , Wang Mei , Ge Jiaojiao 
Published: October 2018

Objective This review summarizes the effects of aerobic exercise (AE), resistance exercise (RE), and high-intensity interval training (HIIT) on nonalcoholic fatty liver disease (NAFLD).Discuss The main differences of intervention methods, intervention time, and intervention effects among the NAFLD people, To explore the targeting and dose-response relationship of different exercise models intervention in NAFLD.
Methods This paper uses the keywords such as Non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, NAFLD, NASH, NAFLD and aerobic training, aerobic exercise, resistance training, resistance exercise, high-intensity interval training, high-intensity interval exercise, high-intensity aerobic exercise, HIAT, HIIT, HIT, etc to search in the web of science、Pubmed database, the deadline is March 1, 2018. Inclusion criteria: (1) study design: randomized controlled trials; (2) study questions: effects of exercise on hepatic steatosis in patients with NAFLD; (3) type of exercise: AE, RE, and HIIT; (4) subjects: biochemical examination liver biopsy or ultrasound examination includes a combination of abdominal imaging and magnetic resonance imaging (MRI) imaging of the NAFLD population. Exclusion criteria: (1) non-original studies (2) meeting abstracts (3) did not provide sufficient experimental data (4) animal experiments (5) non-English literature. The search results showed that a total of 16 studies reported the effects of exercise on NAFLD populations.
Results (1) AE, RE and HIIT can reduce hepatic steatosis and improve liver histology in NAFLD people, but their intervention effects are different. AE stands out in reduce body weight,decreases insulin resistance (IR); RE stands out in reduce hepatic fat and increases muscle strength; HIIT has a significant effect in reducing hepatic fat and enhancing cardiovascular fitness. (2) the frequency, duration, and intervention period of AE and RE are similar; achieve the same or better intervention effect, HIIT only requires the 1/3 exercise time of the previous two.
Conclusions RE may be more effective than AE in patients with poor cardiovascular fitness, sarcopenia, and NAFLD who are unable to tolerate or participate in AE; HIIT has certain advantages in the time-effect and dose-effect due to less exercise time and smaller amount of exercise, This is easy for the NAFLD people to accept, and it will facilitate long-term adherence in the future.
PO-137 Epigenetic regulation of exercise-improved LTCC and BKCa channels function in hypertension mesenteric arteries
Authors: Zhang Yanyan , Shi Lijun 
Published: October 2018

Objective To investigate the epigenetic mechanism of the voltage-gated L-type Ca2+ channel (LTCC) and the large-conductance Ca2+-activated K+ channel (BKCa) function in mesenteric arterial myocytes improved by regular aerobic exercise in hypertension.
Methods 12-week-old male SHR and WKY rats were randomly assigned to sedentary and exercise training groups, respectively. Exercise groups were performed a moderate-intensity treadmill running. After 8 weeks, patch clamp study, Ca2+ image, Western blot, qPCR, bisulfite sequencing PCR were used to detect the LTCC and BKCa channel currents, BKCa single channel gating properties, Ca2+ spark, mRNA and protein expression of LTCC α1c together with BKCa α and β1 subunits, DNA methylation level of α1c and β1 gene promoter region, miR-328 expression. In vitro experiment，miR-328 mimic and miR-328 inhibitor were transfected into cultured arterial myocytes to make miR-328 overexpressing or silencing, the mRNA and protein expression of α1c subunits were determined after 48 h transfection.
Results 1) After 8 weeks of exercise, SBP in both exercise groups of WKY and SHR were significantly lower than that of their sedentary counterparts. 2) Exercise normalized the increased LTCC and BKCa current density of mesenteric arterial myocytes in SHR. 3) Exercise attenuated the increased single BKCa channel open Probability (Po) and the amplitude of Ca2+ spark in hypertension. 4) Exercise inhibited the upregulated mRNA and protein expression of BKCa β1 subunit in mesenteric arteries from SHR; β1 gene promoter was demethylation in hypertension, exercise increased the methylation level at β1 gene promoter of SHR. 5) The protein expression of LTCC α1c subunit was significantly increased in SHR, while decreased by exercise; the expression of miR-328 in mesenteric arteries was highly negative correlation with α1c subunit. 6) The miR-328 overexpression by transfecting miR-328 mimic decreased α1c subunit protein level significantly, while miR-328 inhibitor made α1c subunit a slight increase.
Conclusions Regular aerobic exercise efficiently reduces blood pressure of SHR, enhances β1 gene promoter methylation, mediates miR-328 inhibiting the α1c expression at post-transcriptional level, which might be the epigenetic mechanism underlying exercise-improved LTCC and BKCa channels function in mesenteric arteries of hypertension.
PO-144 Intermittent Exercise Activates NRG1-SERCA2a Pathway to Improve Cardiac Function in Myocardial Infarction Rats
Authors: Bo Wenyan , Li Dagang , Tian Zhenjun 
Published: October 2018

Objective Intermittent exercise can improve cardiac function in rats with myocardial infarction. The Neuregulin-1(NRG1)/SERCA2a palys a critical role in maintain cardiac function. We want to investigate the effect of Neuregulin-1 (NRG1) on NRG1-SERCA2a signaling pathway activated by intermittent exercise and on improves cardiac function in rats with MI.
Methods 32 male sprague-dawley rats were randomly divided into four groups (n=8): Sham-operated group (S), sedentary MI group (MI), MI with interval training group (ME), ME with inhibitor AG1478 group (MA). ME and MA model after the MI model was established by ligation of the left anterior descending coronary artery, and began training 1 week after MI surgery. The S model only by threading without ligation. Rats in ME and MA model taken one week adaptive training, then began 8-week interval training. MA model were injected with inhibitor AG1478, once every two days. The 24h after training, rats were anesthetized, the LVSP, LVEDP, ±dp/dt max were tested by carotid artery intubation which in order to evaluate cardiac function. The protein expression of NRG1, PI3K, Akt, eNOS, PKG, PLN, SERCA2a in myocardium were measured by Westernblotting, themRNA expression of serca2a were tested by RT-qPCR.
Results Compared with S, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a decreased, serca2a mRNA expression decreased, LVSP and ±dp/dt max significantly decreased, LVEDP significantly increased; Compared with MI, the protein expression of NRG1, PKG, peNOS, pAkt, pPLN, pPI3K and SERCA2a increased, serca2a mRNA expression increased, LVSP and ±dp/dt max significantly increased, LVEDP decreased, and the effect of exercise were weaken by inhibitor AG1478. Correlation analysis showed that the myocardial pPLN and SERCA2a protein expression both were positively correlated with LVSP, ±dp/dtmax, and negatively correlated with LVEDP.
Conclusions Intermittent exercise can increased myocardial NRG1 protein expression and activates NRG1-SERCA2a signaling pathway, improve myocardial infarction cardiac function.
PO-190 Exercise Training Protects Against Cardiac Pathological Remodeling in Myocardial Infarction rats via Improving Mitochondrial Biogenesis
Authors: Jia Dandan , Tian Zhenjun 
Published: October 2018

Objective Growing evidence suggests that exercise training reverses cardiac pathological remodeling and cardiac dysfunction during myocardial infarction (MI), but the underlying mechanisms have not been fully understood. In this study, we investigated the impact of exercise training on cardiac function, myocardial fibrosis, apoptosis, oxidative stress and mitochondrial biogenesis.
Methods Sprague Dawley rats were subjected to MI by permanent ligation of the left anterior descending (LAD) coronary artery or Sham operation. Rats with MI were randomly assigned to sedentary MI group (MI) and MI with exercise training group (MI+EX), and compared to sham-operated group (Sham). Haemodynamics and Masson staining were conducted to evaluate the effect of exercise training on cardiac function and myocardial fibrosis. Myocardial apoptosis, oxidative stress, mitochondrial biogenesis and molecular signaling mechanism were analyzed.
Results Exercise training significantly improves cardiac function and mitigates the MI-induced cardiac pathological remodeling. Meanwhile, Exercise training significantly attenuates MI-induced apoptosis, oxidative stress and mitochondrial biogenesis. In addition, activation of PI3K pathway following MI is further induced by exercise training.
Conclusions Exercise training protects against MI-induced cardiac dysfunction and pathological remodeling through preventing myocardial apoptosis and oxidative stress, and enhancing mitochondrial biogenesis.
KONTRIBUSI PERADILAN ADAT DAN KEADILAN RESTORATIF DALAM PEMBARUAN HUKUM PIDANA DI INDONESIA
Authors: Rochaeti Nur , Sutanti Rahmi Dwi 
Published: October 2018

Keadilan menurut masyarakat adat dimaknai sebagai suatu konsep yang bersifat kompleks, karena tidak hanya dapat diberikan oleh pengadilan formal, namun juga dapat diberikan oleh forum lain seperti peradilan adat. Penelitian ini bertujuan untuk menganalisa kontribusi peradilan adat dan keadilan restoratif dalam pembaruan hukum pidana di Indonesia. Pendekatan yang digunakan dalam penelitian ini adalah kajian sosio-legal (socio-legal study).Hasil penelitian menunjukkan peradilan adat dan keadilan restoratif di Indonesia telah dilakukan melalui partisipasi anggota masyarakat dengan karakteristik tradisional hukum, pluralisme budaya, nilai-nilai moral, dan agama dalam rangka memecahkan masalah melalui konsultasi untuk mencapai kesepakatan. Peradilan adat dan keadilan restoratif di Indonesia dilakukan dengan mediasi untuk kesepakatan untuk mencapai kesepakatan demi kepentingan terbaik bagi korban, pelaku, keluarga, dan pihak-pihak lainnya yang berkaitan dengan karakteristik tradisional hukum, pluralisme budaya, nilai-nilai moral, dan agama.
DISPARITAS DALAM PENJATUHAN PIDANA
Authors: Gulo Nimerodi 
Published: October 2018

Rumusan norma yang berkaitan dengan ancaman pidana pada dasarnya bersifat maksimum. Hal tersebut menimbulkan ruang disparitas putusan hakim. Disapritas tersebut dapat menimbulkan rasa ketidakadilan (keadilan substantif) bagi terpidana. Rumusan masalahnya adalah, apa yang menjadi faktor penyebab terjadinya disparitas pidana dalam penjatuhan pidana yang dilakukan oleh hakim dan apakah yang menjadi dasar pertimbangan hakim dalam memutus perakara pidana didalam persidangan?. Metode penelitian yang digunakan adalah metode normatif dan empiris dalam kaitannya dengan keadilan substantif. Hasil penelitian menunjukan dalam memutuskan perkara hakim tunduk pada Pasal 197 KUHAP, yaitu hakim harus memiliki pertimbangannya sendiri didalam menentukan berat atau ringannya hukuman yang akan dijatuhkan kepada terdakwa, melalui pembuktian materil dipersidangan untuk mendukung kesimpulan dalam pertimbangan hakim. Saat ini peradilan di Indonesia masih menggunakan metode penjatuhan hukuman berdasarkan pemeriksaan persidangan saja. Hal ini menyebabkan putusan pengadilan yang dikeluarkan oleh hakim terdapat perbedaan antara satu putusan dengan putusan yang lainnya yang disebut dengan disparitas pidana.