bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of neutrophils in the pathogenesis of Post Kala-azar Dermal Leishmaniasis (PKDL)
Authors: Roy Madhurima , Sengupta Ritika , Chakraborty Bidhan Chandra , Chatterjee Uttara , Stebut Esther von , Kaye Paul M. , Chatterjee Mitali 
Published: November 2024

Background
Post Kala-azar Dermal Leishmaniasis (PKDL) is a dermal sequel of visceral leishmaniasis (VL), poses a significant threat to the success of ongoing kala-azar elimination program, due to its potential role in sustaining transmission cycles and complicating disease management strategies. In VL, neutrophils have been identified as the ‘first line of defence’, having multiple roles in disease pathogenesis, but their role in PKDL, if any, still remains elusive; presenting a critical gap in knowledge, and was the aim of this study.

Methodology/Principal findings
In a cohort of PKDL patients, CD66b+ neutrophils were quantified in skin biopsies, followed by immunostaining of FFPE sections to identify activated neutrophils (CD66b+/CD64+) and degranulated (CD66b+/MPO+), along with expression of neutrophil elastase (NE), matrix metalloprotease 9 (MMP9) and collagen I. Plasma levels of neutrophil chemo-attractants CXCL8/1/2/5, CCL2 and 20 and cytokines, (IL-6, IFN-γ, IL-4, IL-10, TNF-α, IL-17 and IL-22, 23) were evaluated by a multiplex assay, while lesional expression of IL-8, IL-10 and IL-17 was evaluated by immunohistochemistry. As compared to healthy individuals (control skin samples), PKDL cases at the lesional sites had an increased number of activated CD66b+ neutrophils (positive for CD64+, MPO+ and NE+). The plasma levels of neutrophil chemo-attractants, pro-inflammatory and regulatory cytokines were raised as was circulating and lesional IL-8, along with an enhanced lesional expression of IL-10 and IL-17A. An increase in circulatory and lesional MMP9 was accompanied by decreased collagen I, suggesting disintegration of matrix integrity.

Conclusions/Significance
Taken together, in PKDL, activated neutrophils possibly contribute towards modulating the lesional landscape. Understanding this involvement of neutrophils in patients with PKDL, particularly in the absence of an animal model, could offer better understanding of the disease pathogenesis and provide insights into novel therapeutic strategies for the ongoing elimination program.
Effect of community health education on mothers’ knowledge of obstetric danger signs and birth preparedness and complication readiness practices in southern Ethiopia: A cluster randomized controlled trial
Authors: Yoseph Amanuel , Teklesilasie Wondwosen , Guillen-Grima Francisco , Astatkie Ayalew 
Published: November 2024

Introduction
Increasing knowledge of obstetric danger signs (ODS) and encouraging birth preparedness and complication readiness (BPCR) practices are strategies to increase skilled maternal health service utilization in low-income countries. One of the methods to increase mothers’ knowledge about ODS and promote BPCR practice is through health education intervention (HEI). However, the effect of context-specific community-based health education led by women’s groups on these outcomes has yet to be comprehensively studied, and the existing evidence is inconclusive. Thus, we aimed to evaluate the effect of a context-specific community-based HEI led by women’s groups on mothers’ knowledge regarding ODS and BPCR practices in southern Ethiopia.

Methods
An open-label, two-arm parallel group cluster-randomized controlled trial was conducted from January to August 2023 on pregnant women from 24 clusters (kebeles) (12 interventions and 12 controls) in the northern zone of the Sidama region. The Open Data Kit smartphone application was utilized to collect data. The intention-to-treat analysis was used to compare outcomes between groups. We fitted multilevel mixed-effects modified Poisson regression with robust standard error to account for between and within cluster effects.

Results
One thousand and seventy pregnant women (540 in the intervention and 530 in the control clusters) responded to this study, making the overall response rate 95.02%. Excessive vaginal bleeding (94.3% in the interventional group vs. 88.7% in the control group) was the commonest ODS mentioned during childbirth. Overall, 68.7% of women in the intervention group and 36.2% of mothers in the control group had good knowledge of ODS (P-value < 0.001). Saving money and materials (97.1% in the interventional group vs. 92.7% in the control group) was the most frequently practiced BPCR plan. Overall, 64.3% of women in the intervention group and 38.9% of mothers in the control group practiced BPCR (P-value < 0.001). HEI significantly increased overall knowledge of ODS (adjusted risk ratio [ARR]: 1.71; 99% CI: 1.14–2.57) and improved overall BPCR practice (ARR: 1.55; 99% CI: 1.02–2.39).

Conclusions
A community-based HEI led by women’s groups improved mothers’ knowledge regarding ODS and BPCR practices in a rural setting in southern Ethiopia. Interventions designed to increase women’s knowledge of ODS and improve BPCR practice must implement context-specific, community-based HEI that aligns with World Health Organization recommendations.

Trial registration
NCT05865873.
Deciphering the impact of senescence in kidney transplant rejection: An integrative machine learning and multi-omics analysis via bulk and single-cell RNA sequencing
Authors: Shen Xihao , Wu Jiyue , Zhang Feilong , Bi Qing , Sun Zejia , Wang Wei 
Published: November 2024

Background
The demographic shift towards an older population presents significant challenges for kidney transplantation (KTx), particularly due to the vulnerability of aged donor kidneys to ischemic damage, delayed graft function, and reduced graft survival. KTx rejection poses a significant threat to allograft function and longevity of the kidney graft. The relationship between senescence and rejection remains elusive and controversial.

Methods
Gene Expression Omnibus (GEO) provided microarray and single-cell RNA sequencing datasets. After integrating Senescence-Related Genes (SRGs) from multiple established databases, differential expression analysis, weighted gene co-expression network analysis (WGCNA), and machine learning algorithms were applied to identify predictive SRGs (pSRGs). A cluster analysis of rejection samples was conducted using the consensus clustering algorithm. Subsequently, we utilized multiple machine learning methods (RF, SVM, XGB, GLM and LASSO) based on pSRGs to develop the optimal Acute Rejection (AR) diagnostic model and long-term graft survival predictive signatures. Finally, we validated the role of pSRGs and senescence in kidney rejection through the single-cell landscape.

Results
Thirteen pSRGs were identified, correlating with rejection. Two rejection clusters were divided (Cluster C1 and C2). GSVA analysis of two clusters underscored a positive correlation between senescence, KTx rejection occurrence and worse graft survival. A non-invasive diagnostic model (AUC = 0.975) and a prognostic model (1- Year AUC = 0.881; 2- Year AUC = 0.880; 3- Year AUC = 0.883) for graft survival were developed, demonstrating significant predictive capabilities to early detect acute rejection and long-term graft outcomes. Single-cell sequencing analysis provided a detailed cellular-level landscape of rejection, supporting the conclusions drawn from above.

Conclusion
Our comprehensive analysis underscores the pivotal role of senescence in KTx rejection, highlighting the potential of SRGs as biomarkers for diagnosing rejection and predicting graft survival, which may enhance personalized treatment strategies and improve transplant outcomes.
Australian Communities That Care (CTC) intervention: Benefit-cost analysis of a community-based youth alcohol prevention initiative
Authors: Abimanyi-Ochom Julie , Wanni Arachchige Dona Sithara , Bohingamu Mudiyanselage Shalika , Mehta Kanika , Kuklinski Margaret , Rowland Bosco , Toumbourou John W. , Carter Rob 
Published: November 2024

Background
Internationally, adolescent alcohol consumption has been a major community concern for decades. Globally, there is a growing array of interventions aimed at preventing youth alcohol-related problems. Notably, the Communities that Care (CTC) process in the USA has proven to be a cost-effective intervention, leading to a reduction in adolescent alcohol-related problems. In Australia, the CTC trial has shown positive outcomes such as reduced adolescent alcohol and drug use, antisocial behaviors, and the availability of alcohol in the community. To encourage wider adoption, it is essential to ascertain the cost-effectiveness of the Australian CTC trial and its potential impact on addressing youth alcohol problems.

Method
We conducted a limited societal perspective benefit-cost analysis focused on reducing adolescent (aged 10–14) alcohol use in the initial four CTC communities in Australia, spanning 2001 to 2015. To ensure accuracy, estimated benefits were adjusted for joint effects to prevent the double counting of the benefits over time.

Results
The Australian CTC trial, as an adolescent alcohol prevention strategy, demonstrates robust economic credentials, delivering a return of AUD 2.6 for each dollar invested. The cost savings resulting from the reduction in alcohol consumption were estimated at AUD 123 per youth per 15 years and AUD 8 per person per year. The average cost of the CTC trial amounted to AUD 48 per youth over 15 years and AUD 3 per youth per year. The largest contribution to primary benefits were reductions in crime and violence with 93% of the total benefits.

Conclusions
This study makes a valuable contribution to the international economic evidence related to youth alcohol prevention initiatives. The results affirm the strong cost-effectiveness potential of the initial CTC trial implementation in Australia. Moreover, conducting further cost-benefit modelling for additional outcomes and beyond the intervention’s timeframe is likely to enhance the economic viability of the CTC trial.
Sex Differences in Patient-Reported Outcomes and Perception of Ascites Burden Among Outpatients With Decompensated Cirrhosis and Ascites
Authors: Wong Florence , Reddy K. Rajender , Tandon Puneeta , Lai Jennifer C. , Garcia-Tsao Guadalupe , O'Leary Jacqueline G. , Biggins Scott W. , Vargas Hugo E. , Thacker Leroy , Kamath Patrick S. , Bajaj Jasmohan S. ,   The NACSELD III Study Group
Published: November 2024

INTRODUCTION:
Perception of the ascites burden and its effects on quality of life may be different between sexes. This study assessed sex differences in perception of ascites burden and its impact on health-related quality of life (HRQoL) in patients with recurrent or refractory ascites.

METHODS:
The North American Consortium for the Study of End-stage Liver Disease prospectively enrolled outpatients with cirrhosis and large ascites requiring repeat large volume paracenteses. Demographics, laboratory results, comorbidities, medications, frailty measurements, and self-reported questionnaires related to functional status, physical activities, and HRQoL (generic = Short Form 36 and ascites specific = Ascites Questionnaire) were compared between sexes.

RESULTS:
In total, 392 men (59.6 ± 10.7 years) and 184 women (59.5 ± 11.1 years) with predominantly alcohol-related liver disease (51% and 43%, respectively) and median Model for End-Stage Liver Disease-Na: 13 were enrolled. Both groups had similar comorbidities and cirrhosis complications, ascites duration and severity, and frailty scores (P = 0.94). Women had more symptoms related to their ascites (Ascites Questionnaire score = 66 ± 21 vs 60 ± 21 in men, P = 0.001) (higher value = feeling worse). 35% of women felt depressed vs 22% of men (P = 0.0009), with lower mental but not physical functioning components of Short Form 36 (P = 0.019). Women continued to conduct their daily activities as adequately as men as indicated by Duke Status Activity Index (P = 0.27) and Godin Leisure Activity Index (P = 0.47).

DISCUSSION:
Women with cirrhosis and ascites experienced worse emotional HRQoL than men without difference in daily function. Our analyses underscore the differences in the lived experience of women vs men with cirrhosis and highlight the need for patient-reported metrics to provide patient-centered care.
Technodiscursive Analysis of Twitterbot Poetry
Authors: Waliya Yohanna Joseph , Tijani Mufutau A. 
Published: November 2024

Abstract
Literature in the Twitterverse emerges through two cyber-species: the tweeter and the Twitterbot. Both are fighting for relevance in digital creative writing to win the reader’s attention. They tweet, read, and write to each other remotely, and they correct each other as an algorithmic sharpening symbiosis. This essay will analyse the symmetrical linguistics of Twitterbot poetry that incorporates critical code studies of its source code as a subset of technodiscursive analysis to decipher the meanings of the tweet-poems produced by Leonardo Flores’ @Protestitas. This text contextualises itself in a genre of generative electronic literature with multiple interdisciplinary approaches. Our study focuses on this Twitterbot poetry and its relation to socio-technological communication. We also seek to capture the intention of the Twitterary robopoet @Protestitas through a technodiscursive analysis that consists of what we call “Four-Dimensional Analysis (henceforth 4DAs)” of the Twitterbot poetry. How does Twitterbot produce its output as executed codes? How do we read the language of the tweet-poem? Our study seeks to demystify these phenomena in this article. The analysis deciphers Twitterbot poetry in the manner of the rebus, deconstructing the four semantic elements of the ten excerpts of @Protestitas with its source code. We have compared the source codes with the output on the screen. Therein, we discovered that both of them project the same literary spark.
Study on the morphological characteristics of thermally modified bamboo milling dust
Authors: Cui Yunqi , Qiu Hongru , Ding Tao , Gong Meng , Zhu Nanfeng 
Published: November 2024

Abstract
The hazards of dust are receiving increasing attention with the application of bamboo industrialization. This study focuses on the morphological characteristics and formation mechanisms of milling dust from raw bamboo, dried bamboo, and thermally modified bamboo treated at varying temperatures. The particle size distribution, area-equivalent diameter, minimum Feret diameter, aspect ratio, roundness, and convexity were investigated. A new method combining sieving and image scanning analysis was applied to identify the size and morphology of the dust. The study has found that thermal modification significantly affects particle size and distribution, impacting dust convexity and surface characteristics. Particle size has a greater impact on dust morphology compared to heat treatment temperature. Thermal treatment is shown to degrade hemicellulose, reducing bamboo’s transverse mechanical properties and thereby altering the generated dust. The three-step cutting process is established, including bamboo milling deformation and dust formation by finite element simulation. This study offers a reference for optimizing dust removal ports and enabling real-time adjustments to dust removal system power based on dust morphology.
Persistent hypoglycemia in congenital syphilis: hyperinsulinemic hypoglycemia with a focal pancreatic lesion
Authors: Akyar Mehmet , Guney Varal İpek , Tunç Gaffari , Ören Ayşe , Denkboy Öngen Yasemin , Çağan Eren 
Published: November 2024

Abstract

Objectives
Congenital syphilis can cause severe morbidities such as hydrops fetalis, preterm birth, low birth weight, hepatosplenomegaly, pneumonia, hypoglycemia, etc., and mortality. Despite hypoglycemia being reported in congenital syphilis and hyperinsulinism, only one case was described. In this article, we aimed to present a newborn with congenital syphilis born to a syphilitic mother who was diagnosed with persistent hyperinsulinemic hypoglycemia with a focal lesion during follow-up.

Case presentation
A female patient was born with a weight of 2,450 g in the 32+3rd week of pregnancy from a 30-year-old syphilitic mother who had pleural effusion and ascites, cholestasis, thrombocytopenia, and anemia at birth. Hypoglycemia was detected on the 10th day of the patient’s follow-up (47 mg/dL). The patient was unresponsive to diazoxide, and octreotide, nifedipine, and glucagon treatments were started gradually. No variants were detected in the HH gene panel, but a focal pancreatic head lesion was detected in the pancreatic head in fluorine-18 L-3,4 dihydroxyphenylalanine positron emission tomography-computed tomography. Focal lesionectomy was recommended for the patient.

Conclusions
In this article, we present a neonate with severe early congenital syphilis and focal pancreatic lesions with persistent hyperinsulinemic hypoglycemia unresponsive to diazoxide. Although hypoglycemia can be seen in congenital syphilis and has been reported before, hypoglycemia associated with hyperinsulinism is very rare; only a few patients have been presented, and a focal pancreatic lesion has not been previously reported. The pathology is unknown, but pancreatitis may cause this entity.
That one course: two surveys on multicultural counseling curriculum
Authors: Redmond-Matz Trina , Jocoy Kathleen , Bernhardt Paul C. 
Published: November 2024

Abstract
We surveyed multicultural counseling training practices in graduate level counseling programs in the United States in 2009 and 2020. The initial 2009 investigation focused on masters’ level programs and the characteristics of the respondents’ institutions, counseling programs, and multicultural counseling class (es). Another slightly shorter survey was fielded in 2020 and solicited participation from both masters and doctoral level instructors. Both surveys included questions about the nature of the multicultural counseling curriculum and the instructors’ experiences related to multicultural counseling preparation and teaching. We found while few instructors indicated the presence of more than one multicultural counseling course, broad categories of multicultural issues have been covered in these courses. Skills training, when present, in the form of counseling role-play, appears to be rare and short for most instructors. European American males were in the statistical minority of multicultural counseling instructors, despite representing a large percentage of the counseling population.
La marque comme service ayant une vision propre : une approche sémiotique des architectures de marques
Authors: Perusset Alain 
Published: November 2024

Résumé
Depuis le milieu des années 1990, les spécialistes en brand management recourent à une variété de stratégies de marques fortement institutionnalisées et peu contestées. Or, un examen sémiotique de ces stratégies peut amener à questionner la façon dont ces architectures sont construites. L’objectif de cet article est de mettre en lumière les contradictions sémiotiques d’une partie de ces stratégies pour, d’une part, proposer un modèle d’architecture de marques sémiotiquement fondé, et, d’autre part, offrir une définition renouvelée du concept de marque, comme «service ayant une vision propre». En outre, cet article invite à toujours considérer deux types de services lorsqu’on s’attelle à une réflexion stratégique sur les marques : les « services souverains » et les « services adjoints ». Cette « relation d’appartenance » entre services permet de saisir toutes les relations de marque possibles entre entreprises, filiales, produits, lignes, gammes, offres, etc. Plus généralement, cet article entend valoriser le recours à la sémiotique (post)structurale greimassienne pour enrichir la réflexion et améliorer les pratiques dans le domaine du brand management.
Long-term occupation of bofedales ecosystems by high-Andean hunter-gatherers in the Dry Puna, northern Chile
Authors: Sitzia Luca , Osorio Daniela , García B. Magdalena , Otto Jan-Christoph , Power Ximena , Gayo Eugenia M. , Borie César , Gallardo Matias , Uribe Carlos 
Published: November 2024

Abstract
This project employs a geoarchaeological approach to explore human occupation of the highland wetlands (bofedales) and salt flats of the Dry Puna of northern Chile (>2500m above sea level) during the Holocene. Differences in the archaeological record of each ecosystem are tentatively suggested to relate to settlement patterns and the history of the landscape.
Simulação clínica como ferramenta para o ensino de graduandos de enfermagem: uma revisão integrativa / Clinical simulation as a tool for teaching nursing graduates: an integrative review
Authors: Silva Allana Matos , Silva Carolaine dos Santos , Santos Thalita Silva , Góes Roberta Pereira 
Published: November 2024

Objetivo: identificar na literatura publicações relacionadas à experiência da simulação clínica como ferramenta de ensino para graduandos em Enfermagem. Método: revisão integrativa da literatura, realizada no Portal da Biblioteca Virtual de Saúde através dos Descritores: “Enfermagem”, “Treinamento por simulação” e “Ensino”, com recorte temporal de artigos publicados entre os anos de 2015 e 2020. Resultados: obteve-se 424 artigos, que foram selecionados de forma independente e cega por três pesquisadoras. Após seleção conforme critérios de inclusão e exclusão, obteve-se como amostra final 13 artigos, destes, foi analisado o conteúdo discursivo e, assim, foram levantadas duas categorias temáticas: Elementos essenciais para um bom desenvolvimento da simulação e; Evolução teórico-prática dos estudantes após realização da simulação. Conclusão: as publicações evidenciaram que vivenciar uma experiência que simula um contexto real possibilita transformar os estudantes no centro do processo de ensino, além de auxiliar na aproximação destes ao ambiente profissional. Devido a migração para o sistema OJS3, a íntegra desse artigo pode ser acessada pelo seguinte endereço eletrônico: Simulação clínica como ferramenta para o ensino de graduandos de enfermagem: uma revisão integrativa | Journal of Nursing and Health (ufpel.edu.br)
Fé e espiritualidade no enfrentamento do adoecimento de pacientes clínicos internados em um hospital universitário / Faith and spirituality in coping with illness of patient clinicians admitted to a university hospital
Authors: Silveira de Lima Carolina , Armua Natália da Cunha , De Oliveira Marlon Pereira , Arrieira Isabel Cristina de Oliveira 
Published: November 2024

Objetivo: compreender a relação da fé e/ou espiritualidade no enfrentamento do adoecimento. Método: estudo qualitativo, descritivo e exploratório, realizado com 18 pacientes internados na clínica médica de um hospital universitário no Sul do Brasil. As informações foram coletadas de setembro a outubro de 2019, através de instrumento semiestruturado e categorizadas por meio de análise temática. Resultados: a fé e/ou espiritualidade são recursos importantes evidenciada entre pacientes durante o processo de adoecimento e se manifestam de forma individual podendo variar de acordo com cada cultura, crenças e vivências. Conclusões: a fé e/ou espiritualidade está presente em todos os entrevistados e se fortalece quando alguma doença ameaça modificar a vida. Logo, cabe aos profissionais da saúde reconhecerem esse potencial e incluí-la no plano de cuidado, tornando-o mais humanizado. Devido a migração para o sistema OJS3, a íntegra desse artigo pode ser acessada pelo seguinte endereço eletrônico: Fé e espiritualidade no enfrentamento do adoecimento de pacientes clínicos internados em um hospital universitário | Journal of Nursing and Health (ufpel.edu.br)