bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD147 promotes progression of head and neck squamous cell carcinoma via NF‐kappa B signaling
Authors: Yu Binbin , Zhang Yan , Wu Kailiu , Wang Lizhen , Jiang Yingying , Chen Wantao , Yan Ming 
Published: November 2018

AbstractCD147/basigin (BSG) is highly upregulated in many types of cancer, our previous study has found that CD147/BSG is highly expressed in head and neck squamous cell carcinoma (HNSCC) stem cells, but its role in HNSCC and the underlying mechanism is still unknown. In this study, we investigated the role of CD147 in the progression of HNSCC. Real‐time PCR, western blot and immunohistochemistry were used to detect the expression of CD147 in total 189 HNSCC tissues in compared with normal tissues. In addition, we used proliferation, colony formation, cell cycle and apoptosis, migration and invasion as well as wound‐healing assay to determine the biological roles of CD147 in HNSCC. Then, a xenograft model was performed to evaluate tumor‐promoting and metastasis‐promoting role of CD147 in HNSCC. The results showed that upregulated CD147 expression was associated with aggressive clinicopathologic features in HNSCC. In addition, CD147 promoted proliferation, migration and reduced the apoptosis phenotype of HNSCC cells in vitro as well as tumor initiation and progression in vivo. Furthermore, we demonstrated that CD147 promoted HNSCC progression through nuclear factor kappa B signaling. Therefore, we concluded that CD147 promoted tumor progression in HNSCC and might be a potential prognostic and treatment biomarker for HNSCC.
Epidermolysis bullosa‐specific module of the Infants and Toddlers Dermatology Quality of Life (InToDermQoL) questionnaire
Authors: Chernyshov P.V. , Suru A. , Gedeon I. , Derevyanko L.A. , Tiplica G.S. , Salavastru C.M. 
Published: November 2018

AbstractBackgroundEpidermolysis bullosa (EB) may have severe impact on different aspects of patients’ life. Until now there was no EB‐specific quality of life (QoL) instrument for young children.ObjectiveTo create EB‐specific proxy module of the Infants and Toddlers Dermatology Quality of Life (InToDermQoL) questionnaire.MethodsFocus groups with parents of children with EB were organized. Parents of EB children were interviewed by the project staff with regard to their perception of QoL issues of the skin disease of their children.ResultsFocus groups with parents of EB children in Ukraine and Romania were organized. Parents represented eight boys and 12 girls from 3 months to 4 years old with different EB types and disease severity. Based on the analysis of focus groups’ results, two EB specific items that were not mentioned by parents of children with other skin diseases and therefore were not included to the dermatology‐specific InToDermQoL questionnaire were developed: ‘problems with defecation’ and ‘problems with shoes’. These problems were mentioned by 55% of all parents and 11.76% of parents that represented EB children older than 1 year, respectively.ConclusionWe want to invite other centres and EB related organizations to join our project starting from the pilot test. There are many different reasons why QoL measurement is important in dermatology clinical practice and our goal is practical use of the instrument in children with EB.
HSPB8 over‐expression prevents disruption of blood–brain barrier by promoting autophagic flux after cerebral ischemia/reperfusion injury
Authors: Li Fazhao , Yang Binbin , Li Ting , Gong Xiyu , Zhou Fangfang , Hu Zhiping 
Published: November 2018

AbstractHeat‐shock protein B8 (HSPB8) has been recently reported to confer neuroprotection against ischemia/reperfusion (I/R)‐induced cerebral injury in vivo and in vitro. However, the molecular mechanism is still elusive. This study focused on the effect of intracerebroventricular (i.c.v) delivery of lenti‐HSPB8 virus against neurological injury in a rat model of cerebral I/R and explored the underlying mechanism. We found that lentivirus i.c.v injection‐induced HSPB8 over‐expression strongly alleviated infarct volume, improved neurobehavioral outcomes, and reduced brain edema in rat middle cerebral artery occlusion/reperfusion (MCAO/R) model. Concomitantly, HSPB8 over‐expression noticeably prevented blood–brain barrier (BBB) disruption after cerebral I/R injury as indicated by the reduction in Evans blue leakage and IgG detection in the ipsilateral hemisphere compared with the vehicle group. Moreover, immunoblotting and immunofluorescence staining of tight junction proteins claudin‐5 and occludin showed that HSPB8 over‐expression prevented the degradation of these proteins induced by MCAO/R, which indicated the protective effect of HSPB8 on BBB. Western blotting and immunostaining techniques were also utilized to analyze the expression of the markers of autophagy. We found that HSPB8 over‐expression promoted autophagic flux, evidenced by increased ratio of LC3 I/II, accumulation of Beclin‐1 expression and enhanced p62 degradation. i.c.v injection of 15 μg autophagy inhibitor 3‐methyladenine (3‐MA) was applied at the onset of reperfusion. The results showed that 3‐MA elicited a significant loss of the protective effect of HSPB8 against MCAO/R‐induced neurological defect, Evans blue extravasation, and the loss tight junction proteins, suggesting that the BBB protective role of HSPB8 was, at least in part, mediated through autophagy. Collectively, HSPB8 may represent a potential therapeutic agent for preserving BBB integrity following cerebral I/R injury.Open science badgesThis article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at https://cos.io/our-services/open-science-badges/.
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Cover Image for this issue: doi: 10.1111/jnc.14488.
Impact of local endometrial injury on in vitro fertilization/intracytoplasmic sperm injection outcomes: A systematic review and meta‐analysis
Authors: Gui Juan , Xu Wangming , Yang Jing , Feng Ling , Jia Jing 
Published: November 2018

AbstractAimRecent studies have revealed positive effects of endometrial injury on clinical pregnancy rates, but with inconsistent results. The aim of this meta‐analysis was to assess the efficacy of endometrial injury (biopsy and/or hysteroscopy) as a potential treatment measure for implantation failure in the in vitro fertilization population.MethodsWe searched in PubMed for studies comparing the efficacy of endometrial injury versus no intervention in women with at least one implantation failure. A random‐effects model was used to evaluate the clinical pregnancy rate (CPR) and the live birth rate (LBR).ResultsSeventeen studies (11 randomized and 6 non‐randomized studies) were included in this meta‐analysis, including 1864 women in the intervention group and 2193 women in the control group. Overall, the CPR and LBR were significantly higher in the intervention group than in the control group (for CPR, n = 3997, 16 studies, P < 0.00001, risk ratio (RR) = 1.47, 95% confidence interval [CI] [1.24, 1.74]; for LBR, n = 2361, 11 studies, P = 0.003, RR = 1.41, 95% CI [1.12, 1.78]). However, after excluding the non‐randomized studies, there was no significant difference in CPR (P = 0.29 for endometrial biopsy, P = 0.05 for hysteroscopy) and LBR (P = 0.23 for endometrial biopsy, P = 0.39 for hysteroscopy) between the intervention group and the control group.ConclusionThere is still insufficient evidence to support the use of endometrial injury in women with implantation failure. Robust randomized controlled studies should be designed and performed before clinical implementation of endometrial injury.
Use of Parental Benefits by Family Income in Canada: Two Policy Changes
Authors: Margolis Rachel , Hou Feng , Haan Michael , Holm Anders 
Published: November 2018

Objective: This article examines how two recent policy extensions affected the use and sharing of parental benefits in Canada and how this differed by family income.Background: Paid parental benefits positively affect economic and health outcomes. However, not all policy changes increase leave‐taking, especially among low‐income families.Method: Drawing on administrative data from 1998 to 2012, we estimate linear probability models to examine the likelihood of either parent using parental benefits and multinomial logit models to examine patterns in sharing benefits. We stratify models by household income to examine how the two policy changes affected families differently across the income spectrum.Results: Both policies increased use more among low‐income families than those with higher incomes, which is likely due to widening eligibility criteria that affected low‐income families disproportionately. Second, policy design induced different patterns of sharing benefits in response to the two policy changes. In contrast to the 2001 policy that only moderately increased sharing of parental benefits, Quebec's 2006 program explicitly promoted gender equality and increased sharing of benefits across all income groups, but three times as much for middle‐ and high‐income families than low‐income families.Conclusion: We conclude that policy design shapes socioeconomic inequality in newborns' early life parental context.
Bifactor model of effortful control and impulsivity and their prospective prediction of ego resiliency
Authors: Wang Frances L. , Eisenberg Nancy , Spinrad Tracy L. 
Published: November 2018

AbstractObjectiveChildren’s effortful control and impulsivity are important predictors of the personality trait, ego resiliency (i.e., resiliency). Most researchers have not considered the fact that effortful control and impulsivity share substantial conceptual and empirical overlap, yet they also have been shown to be distinct. We tested a bifactor model of effortful control and impulsivity to characterize their shared and unique variance, the prospective prediction of resiliency by the factors of the bifactor model, and moderation by sex and age.MethodIn a longitudinal study of children (N = 214; 76.5% non‐Hispanic Caucasian, 12.2% Hispanic, 11.3% other race/ethnicity), parent‐ and teacher‐reported effortful control and impulsivity, as well as behavioral measures of effortful control, were assessed on two occasions (T1: 4.5–8 years; T2: 6–10 years). Parent‐reported resiliency was used as a covariate (T1) and the outcome (T3: 8–12 years).ResultsThe bifactor model yielded a common effortful inhibitory control factor, pure attentional control factor, and pure impulsivity factor. Pure impulsivity and pure attentional control positively predicted resiliency, but only for girls. Effortful inhibitory control did not uniquely predict resiliency.ConclusionDisentangling the shared and unique aspects of effortful control and impulsivity could clarify the roles they play in important outcomes, such as resiliency.
Fine‐scale spatial genetic structure in the Minami‐daito Island population of the Ryukyu scops owl Otus elegans
Authors: Sawada A. , Iwasaki T. , Takagi M. 
Published: November 2018

AbstractMultiple factors can give rise to population genetic structure. Therefore, the generating process of genetic structure should be examined from multiple aspects simultaneously. We describe the fine‐scale genetic structure of the Ryukyu scops owl Otus elegans interpositus population on Minami‐daito Island and test isolation‐by‐distance, isolation‐by‐adaptation and assortative mating as candidates for its underlying mechanisms. Spatial principal component analysis detected a spatial autocorrelation structure for males, but not for females. Discriminant analysis of principal components and STRUCTURE analysis allowed us to classify the owl population into six genetically distinct groups. These clustering results were associated with relatedness. In addition, to these descriptive analyses, analyses of mechanisms preferred isolation‐by‐distance to others. Therefore, we inferred that isolation by distance and kinship structure are the most important underlying processes leading to their genetic structure.
Molecular distribution, 13C‐isotope, and enantiomeric compositions of carbonaceous chondrite monocarboxylic acids
Authors: Aponte José C. , Woodward Hannah K. , Abreu Neyda M. , Elsila Jamie E. , Dworkin Jason P. 
Published: November 2018

AbstractThe water‐soluble organic compounds in carbonaceous chondrite meteorites constitute a record of the synthetic reactions occurring at the birth of the solar system and those taking place during parent body alteration and may have been important for the later origins and development of life on Earth. In this present work, we have developed a novel methodology for the simultaneous analysis of the molecular distribution, compound‐specific δ13C, and enantiomeric compositions of aliphatic monocarboxylic acids (MCA) extracted from the hot‐water extracts of 16 carbonaceous chondrites from CM, CR, CO, CV, and CK groups. We observed high concentrations of meteoritic MCAs, with total carbon weight percentages which in some cases approached those of carbonates and insoluble organic matter. Moreover, we found that the concentration of MCAs in CR chondrites is higher than in the other meteorite groups, with acetic acid exhibiting the highest concentration in all samples. The abundance of MCAs decreased with increasing molecular weight and with increasing aqueous and/or thermal alteration experienced by the meteorite sample. The δ13C isotopic values of MCAs ranged from −52 to +27‰, and aside from an inverse relationship between δ13C value and carbon straight‐chain length for C3–C6 MCAs in Murchison, the 13C‐isotopic values did not correlate with the number of carbon atoms per molecule. We also observed racemic compositions of 2‐methylbutanoic acid in CM and CR chondrites. We used this novel analytical protocol and collective data to shed new light on the prebiotic origins of chondritic MCAs.
Impaired microvascular reactivity in gestational diabetes is associated with altered glycemic parameters
Authors: Rahman Aisyah , Munisamy Seetha , Ghaffar Nor Aliza , Mahmood Nik Zaki , Rasool Aida H. G. 
Published: November 2018

AbstractObjectivesTo assess microvascular reactivity and glycemic parameters in GDM compared to age and GA matched controls.MethodsThis study involved 21 GDM patients and 31 controls. Microvascular reactivity was assessed using LDF and PORH. Microvascular parameters; PORHmax, PORHpeak, and time to peak perfusion (Tp) were recorded after the release of 3 minutes’ upper arm occlusion. HOMA‐IR was performed to evaluate insulin resistance.ResultsAverage age and GA for subjects were 32.9 years and 29.2 weeks. Mean FBG and a 2‐hour postprandial for GDM and controls were 4.87 ± 0.71 vs 3.99 ± 0.59 mmol/L; P < 0.001 and 9.50 ± 1.8 vs 5.67 ± 1.0 mmol/L; P < 0.001. Fasting insulin (13.88 ± 18.9 vs 8.37 ± 11.0 μLU/mL; P = 0.031) and HOMA‐IR (3.14 ± 4.6 vs 1.52 ± 2.2; P = 0.004) were higher in GDM. Tp was prolonged in GDM (16.27 ± 4.3 vs 13.86 ± 2.1 seconds; P = 0.011). Positive correlations were seen between Tp and FBG and 2‐hour postprandial levels.ConclusionTp was prolonged in GDM compared to age‐matched controls, indicating impaired microvascular reactivity.
Demography and genetics suggest reversal of dolphin source‐sink dynamics, with implications for conservation
Authors: Manlik Oliver , Chabanne Delphine , Daniel Claire , Bejder Lars , Allen Simon J. , Sherwin William B. 
Published: November 2018

AbstractThe forecast for the viability of populations depends upon metapopulation dynamics: the combination of reproduction and mortality within populations, as well as dispersal between populations. This study focuses on an Indo‐Pacific bottlenose dolphin (Tursiops aduncus) population in coastal waters near Bunbury, Western Australia. Demographic modeling of this population suggested that recent reproductive output was not sufficient to offset mortality. Migrants from adjacent populations might make up this deficit, so that Bunbury would act as a “sink,” or net recipient population. We investigated historical dispersal in and out of Bunbury, using microsatellites and mitochondrial DNA of 193 dolphins across five study locations along the southwestern Australian coastline. Our results indicated limited gene flow between Bunbury and adjacent populations. The data also revealed a net‐dispersal from Bunbury to neighboring populations, with microsatellites showing that more than twice as many individuals per generation dispersed out of Bunbury than into Bunbury. Therefore, in historic times, Bunbury appears to have acted as a source population, supporting nearby populations. In combination with the prior finding that Bunbury is currently not producing surplus offspring to support adjacent populations, this potential reversal of source‐sink dynamics may have serious conservation implications for Bunbury and other populations nearby.
Two venom allergen‐like proteins, HaVAP1 and HaVAP2, are involved in the parasitism of Heterodera avenae
Authors: Luo Shujie , Liu Shiming , Kong Lingan , Peng Huan , Huang Wenkun , Jian Heng , Peng Deliang 
Published: November 2018

SummaryDespite the fact that venom allergen‐like proteins (VAPs) have been identified in many animal‐ and plant‐parasitic nematodes, studies on VAPs in Heterodera avenae, which is an important phytonematode, are still in their infancy. Here, we isolated, cloned and characterized two VAPs, named HaVAP1 and HaVAP2, from H. avenae. The two encoded proteins, HaVAP1 and HaVAP2, harbour an SCP‐like domain each, but share only 38% identity with each other. HaVAP1 and HaVAP2 are expressed in subventral and dorsal oesophageal glands, respectively. HaVAP1 is expressed mainly at the early stages, whereas HaVAP2 accumulates principally at the late stages. Both HaVAP1 and HaVAP2 are secreted when expressed in Nicotiana benthamiana leaves, but HaVAP1 is delivered into chloroplasts, whereas HaVAP2 is translocated to the nucleus without signal peptides. Knocking down HaVAP1 increased the virulence of H. avenae. In contrast, silencing of HaVAP2 hampered the parasitism of H. avenae. Both HaVAP1 and HaVAP2 suppressed the cell death induced by BAX in N. benthamiana leaves. Moreover, HaVAP2 physically interacted with a CYPRO4‐like protein (HvCLP) of Hordeum vulgare in the nucleus of the plant. It is reasonable to speculate that the changes in the transcript of HvCLP are associated with HaVAP2 during the parasitism of H. avenae. All results obtained in this study show that both HaVAP1 and HaVAP2 are involved in the parasitism of H. avenae, but they possess different functions, broadening our understanding of the parasitic mechanism of H. avenae.
The First ‘European’ Writing: Redefining the Archanes Script
Authors: Decorte Roeland P.‐J. E. 
Published: November 2018

SummaryThis paper investigates a series of glyptic inscriptions attested on Crete at the end of the third and beginning of the second millennium BC, collectively referred to as the ‘Archanes Script’. These minute engravings are considered to represent the earliest appearance of writing west of Egypt, and the first ‘true’ writing in the Aegean. Though mentioned in passing in almost every study of Bronze Age Aegean writing, few scholars have ever offered a definition of what exactly they consider the ‘Archanes Script’ to be. No work or scholarly consensus exists delineating which signs constitute its signary, or even which documents comprise its corpus. Study of the seals as objects in their own right, examining script signs alongside associated iconography, material qualities and form, has been rare. This paper offers the first complete overview and redefinition of the Archanes Script since its discovery in the 1960s and initial definition by Paul Yule in 1980.
Glucosylation of (Z)‐3‐hexenol informs intraspecies interactions in plants: A case study in Camellia sinensis
Authors: Jing Tingting , Zhang Na , Gao Ting , Zhao Mingyue , Jin Jieyang , Chen Yongxian , Xu Miaojing , Wan Xiaochun , Schwab Wilfried , Song Chuankui 
Published: November 2018

AbstractPlants emit a variety of volatiles in response to herbivore attack, and (Z)‐3‐hexenol and its glycosides have been shown to function as defence compounds. Although the ability to incorporate and convert (Z)‐3‐hexenol to glycosides is widely conserved in plants, the enzymes responsible for the glycosylation of (Z)‐3‐hexenol remained unknown until today. In this study, uridine‐diphosphate‐dependent glycosyltransferase (UGT) candidate genes were selected by correlation analysis and their response to airborne (Z)‐3‐hexenol, which has been shown to be taken up by the tea plant. The allelic proteins UGT85A53‐1 and UGT85A53‐2 showed the highest activity towards (Z)‐3‐hexenol and are distinct from UGT85A53‐3, which displayed a similar catalytic efficiency for (Z)‐3‐hexenol and nerol. A single amino acid exchange E59D enhanced the activity towards (Z)‐3‐hexenol, whereas a L445M mutation reduced the catalytic activity towards all substrates tested. Transient overexpression of CsUGT85A53‐1 in tobacco significantly increased the level of (Z)‐3‐hexenyl glucoside. The functional characterization of CsUGT85A53 as a (Z)‐3‐hexenol UGT not only provides the foundation for the biotechnological production of (Z)‐3‐hexenyl glucoside but also delivers insights for the development of novel insect pest control strategies in tea plant and might be generally applicable to other plants.
Haplotypic differentiation between seasonal populations of Sargassum horneri (Fucales, Phaeophyceae) in Japan
Authors: Watanabe Kohei , Homma Yuri , Karakisawa Hideyuki , Ishikawa Ryoko , Uwai Shinya 
Published: November 2018

SUMMARYConspecific populations with different life‐cycle seasonality, particularly of their reproductive season, have been reported for many seaweed species. However, the number of genetic analyses of such seasonal populations is limited. Herein, based on the mitochondrial cytochrome oxidase subunit 3 gene (cox3) haplotype, we evaluated the genetic diversity of winter (January–March) and spring (April–June) fertile populations of the brown alga Sargassum horneri in Sado Island, Japan. Obviously, dominant cox3 haplotypes were different between seasons (even at a single site), and the N
ST values indicated the presence of a strong genetic differentiation between the two seasonal populations. AMOVA analyses confirmed that most of the genetic diversity was between seasons rather than between regions. Herein, the possibility of allochronic isolation is discussed. Considering the phylogeny of the mitochondrial cox3 DNA sequence, autumn or winter seasonal populations such as those in this investigation have evolved independently at least twice in the species. The present study shows that a shift in breeding season could be expected to conserve the genetic diversity of S. horneri in a certain region.
Blood pressure target for the dialysis patient
Authors: McCallum Wendy , Sarnak Mark J. 
Published: November 2018

AbstractThe appropriate blood pressure (BP) target for dialysis patients remains controversial. Although there have been remarkable advances in this area in the general population, extrapolation of these data to dialysis patients is not possible. Observational studies in dialysis patients suggest that low BP is associated with worse outcomes. However, this is likely a result of confounding, considering that among dialysis patients with fewer cardiovascular comorbidities and longer survival, a more linear relationship exists between BP and mortality. Use of home BP measurements and ambulatory blood pressure monitoring (ABPM) measurements are more useful from a prognostic standpoint than in‐center predialysis BP measurements. Large clinical trial data are, however, lacking and firm recommendations on BP targets for dialysis patients are not possible.