bluuebunny/crossref_metadata_2025_split huggingface dataset:
What Police Say about Crime Prevention Through Environmental Design Training in Two Jurisdictions (England/Wales and New South Wales, Australia)
Authors: Monchuk Leanne , Clancey Garner 
Published: March 2019

Abstract
Crime Prevention Through Environmental Design (CPTED) aims to reduce crime and disorder through the design and manipulation of the built environment. Various professions and agencies practice CPTED. The police typically play an important role in the delivery of CPTED by assessing planning applications, identifying criminogenic design features, and offering remedial advice. As with other areas of policing, the training and professional development opportunities have received limited academic attention. This article reports on data collected from studies conducted in England and Wales and in New South Wales (Australia). A stratified sample of 30 Designing out Crime Officers from England and Wales and a convenience sample of 36 Crime Prevention Officers from New South Wales Police Force were interviewed and their views on CPTED training and development sought. It will be seen that this comparative analysis highlights similar concerns amongst those responsible for delivering CPTED, regardless of jurisdiction and professional background. Concerns related to the limited training and continued professional development opportunities that assist officers in undertaking the role and the level of confidence with which they feel able to advocate CPTED recommendations. This article briefly questions the utility of operational policing to do this role, but if they do, they need to be adequately resourced, which includes the provision of a dedicated training and continued professional development programme.
OCCUPATIONAL RADIATION DOSE TO NUCLEAR MEDICINE STAFF DUE TO TC99M, F18-FDG PET AND THERAPEUTIC I-131 BASED EXAMINATIONS
Authors: Fathy Mohamed , Khalil Magdy M , Elshemey Wael M , Mohamed Haitham S 
Published: March 2019

Abstract
The aim was to track the exposure to radiation workers in six nuclear medicine examinations. A number of 180 patients were recruited and external exposure was measured. Patients had undergone cardiac stress and rest, bone scan, I-131 therapy, Gallium-67 and FDG PET/CT imaging. The average dose received due to cardiac stress and rest were 20.4 ± 5.0 and 16.0 ± 3.8 μSv per patient, respectively, whereas for bone scan, Ga-67, FDG and I-131 therapy, the average dose was 6.1 ± 2.5, 6.0 ± 1.4, 11.1 ± 2.2 and 4.1 ± 2.6 μSv per patient. The patient-to-staff dose coefficients were on average 0.051 ± 0.009, 0.042 ± 0.010, 0.034 ± 0.016, 0.039 ± 0.021, 0.052 ± 0.012, 0.094 ± 0.021 μSv m2/MBq h for stress, rest, bone, I-131, Ga-67 and FDG reported post-administration, respectively. Patient injection and setup for imaging represent a high percentage of the total dose received by staff. The information revealed is able to revise local measures, safety standards, and could help further in dose optimization and minimal exposure to occupationally exposed worker in nuclear medicine laboratories.
From social brains to social robots: applying neurocognitive insights to human–robot interaction
Authors: Cross Emily S. , Hortensius Ruud , Wykowska Agnieszka 
Published: March 2019

Amidst the fourth industrial revolution, social robots are resolutely moving from fiction to reality. With sophisticated artificial agents becoming ever more ubiquitous in daily life, researchers across different fields are grappling with the questions concerning how humans perceive and interact with these agents and the extent to which the human brain incorporates intelligent machines into our social milieu. This theme issue surveys and discusses the latest findings, current challenges and future directions in neuroscience- and psychology-inspired human–robot interaction (HRI). Critical questions are explored from a transdisciplinary perspective centred around four core topics in HRI: technical solutions for HRI, development and learning for HRI, robots as a tool to study social cognition, and moral and ethical implications of HRI. Integrating findings from diverse but complementary research fields, including social and cognitive neurosciences, psychology, artificial intelligence and robotics, the contributions showcase ways in which research from disciplines spanning biological sciences, social sciences and technology deepen our understanding of the potential and limits of robotic agents in human social life.
This article is part of the theme issue ‘From social brains to social robots: applying neurocognitive insights to human–robot interaction’.
Would a robot trust you? Developmental robotics model of trust and theory of mind
Authors: Vinanzi Samuele , Patacchiola Massimiliano , Chella Antonio , Cangelosi Angelo 
Published: March 2019

Trust is a critical issue in human–robot interactions: as robotic systems gain complexity, it becomes crucial for them to be able to blend into our society by maximizing their acceptability and reliability. Various studies have examined how trust is attributed by people to robots, but fewer have investigated the opposite scenario, where a robot is the trustor and a human is the trustee. The ability for an agent to evaluate the trustworthiness of its sources of information is particularly useful in joint task situations where people and robots must collaborate to reach shared goals. We propose an artificial cognitive architecture based on the developmental robotics paradigm that can estimate the trustworthiness of its human interactors for the purpose of decision making. This is accomplished using Theory of Mind (ToM), the psychological ability to assign to others beliefs and intentions that can differ from one’s owns. Our work is focused on a humanoid robot cognitive architecture that integrates a probabilistic ToM and trust model supported by an episodic memory system. We tested our architecture on an established developmental psychological experiment, achieving the same results obtained by children, thus demonstrating a new method to enhance the quality of human and robot collaborations.This article is part of the theme issue ‘From social brains to social robots: applying neurocognitive insights to human–robot interaction’.
Constrained Transcriptional Polarity in the Organization of MammalianHoxGene Clusters
Authors: Darbellay Fabrice , Bochaton Célia , Lopez-Delisle Lucille , Mascrez Bénédicte , Tschopp Patrick , Delpretti Saskia , Zakany Jozsef , Duboule Denis 
Published: March 2019

ABSTRACTIn many animal species with a bilateral symmetry,Hoxgenes are clustered either at one or at several genomic loci. This organization has a functional relevance, as the transcriptional control applied to each gene depends upon its relative position within the gene cluster. It was previously noted that vertebrateHoxclusters display a much higher level of genomic organization than their invertebrate counterparts. The former are always more compact than the latter, they are generally devoid of repeats and of interspersed genes, and all genes are transcribed by the same DNA strand, suggesting that particular factors constrained these clusters towards a tighter structure during the evolution of the vertebrate lineage. Here we investigate the importance of uniform transcriptional orientation by engineering several alleles within theHoxDcluster such as to invert one or several transcription unit(s), with or without a neighboring CTCF site. We observe that the association between the tight structure of mammalianHoxclusters and their regulation makes inversions likely detrimental to the proper implementation of this complex genetic system. We propose that the consolidation ofHoxclusters in vertebrates, including transcriptional polarity, evolved in conjunction with the emergence of global gene regulationviathe flanking regulatory landscapes, to optimize a coordinated response of selected subsets of target genes incis.
Numerical simulations of different models describing cerebrospinal fluid dynamics
Authors: Romagnoli Licia 
Published: March 2019

AbstractThe aim of this paper is to present an extensive overview of numerical simulations aimed at confirming and completing the theoretical results obtained in the analysis of some cerebrospinal fluid dynamics models which are treated from a purely mathematical view point. The present study is designed to support the first attempts in the approach to these physiological models from a more theoretical standpoint since their investigation in literature only concerns the modelization and the clinical feedback.
Phased genome sequence of an interspecific hybrid flowering cherry, Somei-Yoshino (Cerasus × yedoensis)
Authors: Shirasawa Kenta , Esumi Tomoya , Hirakawa Hideki , Tanaka Hideyuki , Itai Akihiro , Ghelfi Andrea , Nagasaki Hideki , Isobe Sachiko 
Published: March 2019

AbstractWe report the phased genome sequence of an interspecific hybrid, the flowering cherry Somei-Yoshino (Cerasus × yedoensis). The sequence was determined by single-molecule real-time sequencing technology and assembled using a trio-binning strategy in which allelic variation was resolved to obtain phased sequences. The resultant assembly consisting of two haplotype genomes spanned 690.1 Mb with 4,552 contigs and an N50 length of 1.0 Mb. We predicted 95,076 high-confidence genes, including 94.9% of the core eukaryotic genes. Based on a high-density genetic map, we established a pair of eight pseudomolecule sequences, with highly conserved structures between two genome sequences with 2.4 million sequence variants. A whole genome resequencing analysis of flowering cherry varieties suggested that Somei-Yoshino is derived from a cross between C. spachiana and either C. speciose or its derivative. Transcriptome data for flowering date revealed comprehensive changes in gene expression in floral bud development toward flowering. These genome and transcriptome data are expected to provide insights into the evolution and cultivation of flowering cherry and the molecular mechanism underlying flowering.
PRDM16 establishes lineage-specific transcriptional program to promote temporal progression of neural progenitors in the mouse neocortex
Authors: He Li , Jones Jennifer , He Weiguo , Bjork Bryan , Wen Jiayu , Dai Qi 
Published: March 2019

AbstractRadial glia (RG) in the neocortex sequentially generate distinct subtypes of projection neurons, accounting for the diversity and complex assembly of cortical neural circuits. Mechanisms that drive the rapid and precise temporal progression of RG are beginning to be elucidated. Here we reveal that the RG-specific transcriptional regulator PRDM16 promotes the transition of early to late phases of neurogenesis in the mouse neocortex.Prdm16mutant RG delays the timely progression of RG, leading to defective cortical laminar organization. We show that PRDM16 regulates expression of neuronal specification genes and a subset of genes that are dynamically expressed between mid-and late-neurogenesis. Our genomic analysis suggests that PRDM16 suppresses target gene expression through maintaining chromatin accessibility of permissive enhancers. Altogether, our results demonstrate a critical role of PRDM16 in establishing stage-specific gene expression program of RG during cortical neurogenesis. These findings also support a model where progenitor cells are primed with daughter cell gene expression program for rapid cell differentiation.
Gut microbiome structure and adrenocortical activity in dogs with aggressive and phobic behavioral disorders
Authors: Mondo E , Barone M , Soverini M , D’Amico F , Cocchi M , Petrulli C , Mattioli M , Marliani G , Candela M , Accorsi PE 
Published: March 2019

AbstractAccompanying human beings since the Paleolithic period, dogs has been recently regarded as a reliable model for the study of the gut microbiome connections with health and disease. In order to provide some glimpses on the connections between the gut microbiome layout and host behavior, we profiled the phylogenetic composition and structure of the canine gut microbiome of dogs with aggressive (n = 17), phobic (n = 15) and normal behavior (n = 17). According to our findings, aggressive behavioral disorder was found to be characterized by a peculiar gut microbiome structure, with high biodiversity and enrichment in generally subdominant bacterial genera. On the other hand, phobic dogs were enriched inLactobacillus, a bacterial genus with known probiotic and psychobiotic properties. Although further studies are needed to validate our findings, our work supports the intriguing opportunity that different behavioral phenotypes in dogs may be associated with peculiar gut microbiome layouts, suggesting possible connections between the gut microbiome and the central nervous system and indicating the possible adoption of probiotic interventions aimed at restoring a balanced host-symbiont interplay for mitigating behavioral disorders.
Recombination-independent recognition of DNA homology for meiotic silencing in Neurospora crassa
Authors: Rhoades Nicholas , Nguyen Tinh-Suong , Witz Guillaume , Cecere Germano , Hammond Thomas , Mazur Alexey K. , Gladyshev Eugene 
Published: March 2019

ABSTRACTPairing of homologous chromosomes represents a critical step of meiosis in nearly all sexually reproducing species. While in some organisms meiotic pairing requires programmed DNA breakage and recombination, in many others it engages homologous chromosomes that remain apparently intact. The mechanistic nature of such recombination-independent pairing represents a fundamental question in molecular genetics. Using ‘meiotic silencing by unpaired DNA’ (MSUD) in Neurospora crassa as a model process, we demonstrate the existence of a cardinally different approach to DNA homology recognition in meiosis. The main advantage of MSUD over other experimental systems lies in its ability to identify any relatively short DNA fragment lacking a homologous allelic partner. Here we show that MSUD does not rely on the canonical mechanism of meiotic recombination, yet it is promoted by REC8, a conserved component of the meiotic cohesin complex. We also show that certain patterns of interspersed homology are recognized as pairable during MSUD. Such patterns need to be co-linear and must contain short tracts of sequence identity spaced apart with a periodicity of 21 or 22 base-pairs. By using these values as a guiding parameter in all-atom molecular modeling, we discover that homologous double-stranded DNA molecules can associate by forming quadruplex-based contacts with an interval of 2.5 helical turns, which requires right-handed plectonemic coiling and additional conformational changes in the intervening double-helical segments. These results (i) reconcile genetic and biophysical lines of evidence for the existence of direct homologous dsDNA-dsDNA pairing, (ii) identify a role for this process in initiating post-transcriptional silencing, and (iii) suggest that chromosomes are cross-matched in meiosis by a precise mechanism that operates on intact double-stranded DNA molecules.
Effects of a demand optimization intervention on laboratory test utilization in primary care
Authors: Bucholc Magda , O’Kane Maurice , O’Hare Brendan , Mullan Ciaran , Cavanagh Paul , Ashe Siobhan 
Published: March 2019

AbstractThere is evidence of increasing use of laboratory tests with substantial variation between clinical teams which is difficult to justify on clinical grounds. The aim of this project was to assess the effect of a demand optimisation intervention project on laboratory test requesting by general practitioners (GPs) in an area of Northern Ireland supported by the Clinical Chemistry Laboratory service of Western Health and Social Care Trust (WHSCT). The intervention package was developed in conjunction with the Western Local Commissioning Group and consisted of educational initiatives, feedback to 55 individual practices on test request rates with ranking relative to other practices, and a small financial incentive for practices to reflect on their test requesting activity. Overall test utilization rates of profile tests, HbA1c, and PSA one year before, during, and one year after the intervention were measured using laboratory databases of the Altnagelvin Area Hospital, Tyrone County Hospital, and the Erne (South West Acute Hospital. The intervention was associated with mixed effects. First, we observed a reduction of 5.1% in the median profile test request rates and a decrease in their between practice variability. The overall downward trend in variability of profile test request rates was found statistically significant (p= 0.03). Second, we found a significant increase in both the volume (p< 0.0001) and between practice variability (p= 0.0001) of HbA1crequests per patient with diabetes. The increase in HbA1crequests may reflect a more appropriate rate of diabetes monitoring and also the adoption of HbA1cas a diagnostic test. Yet, the subsequent 600% increase in between practice variability of HbA1cordering rates may imply an inconsistent implementation of recommended guidelines by GPs. Finally, there was a 29.3% increase in the median and 35% increase in between practice variability of request rates for PSA, the reasons for which are unclear.
The Use of iPSC-Derived Cardiomyocytes and Optical Mapping for Erythromycin Arrhythmogenicity Testing
Authors: Podgurskaya A.D. , Tsvelaya V.A. , Slotvitsky M.M. , Dementyeva E.V. , Valetdinova K.R. , Agladze K.I. 
Published: March 2019

AbstractErythromycin is an antibiotic that prolongs the QT-interval and causes Torsade de Pointes (TdP) by blocking the rapid delayed rectifying potassium current (IKr) without affecting either the slow delayed rectifying potassium current (IKs) or inward rectifying potassium current (IK1). Erythromycin exerts this effect in the range of 1.5 μM–100 μM. However, the mechanism of action underlying its cardiotoxic effect and its role in the induction of arrhythmias, especially in multicellular cardiac experimental models, remain unclear. In this study the re-entry formation, conduction velocity, and maximum capture rate were investigated in a monolayer of human induced pluripotent stem cell (iPSC)-derived cardiomyocytes from a healthy donor and in a neonatal rat ventricular myocyte (NRVM) monolayer using the optical mapping method under erythromycin concentrations of 15, 30, and 45 μM. In the monolayer of human iPSC-derived cardiomyocytes, the conduction velocity (CV) varied up to 12±9% at concentrations of 15–45 μM as compared with that of the control, whereas the maximum capture rate (MCR) declined substantially up to 28±12% (p < 0.05). In contrast, the tests on the NRVM monolayer showed no significant effect on the MCR. The results of the arrhythmogenicity test provided evidence for a “window” of concentrations of the drug (15 to 30 μM) at which the probability of re-entry increased.
De-novovariants inXIRP1associated with polydactyly and polysyndactyly in Holstein cattle
Authors: Braun Marina , Hellige Maren , Gerhauser Ingo , Ciurkiewicz Malgorzata , Lehmbecker Annika , Beineke Andreas , Baumgärtner Wolfgang , Metzger Julia , Distl Ottmar 
Published: March 2019

ABSTRACTCongenital polydactylous cattle are sporadically observed. Impairment of the limb patterning process due to altered control of the zone of polarizing activity (ZPA) was associated in several species with preaxial polydactyly and syndactyly. In cattle, the role of ZPA and other genes involved in limb patterning for polydactyly was not yet elucidated. Herein, we report on a preaxial type II polydactyly and a praeaxial type II+V polysyndactyly in two Holstein calves and screen whole genome sequencing data for associated variants. Using whole genome sequencing data of both affected calves did not show mutations in the candidate regions of ZRS and pZRS or in candidate genes associated with polydactyly, syndactyly and polysyndactyly in other species. Two indels, which are located inXIRP1within a common haplotype, were highly associated with the two phenotypes. Bioinformatic analyses retrieved an interaction betweenXIRP1andFGFR1, CTNNB1andCTNND1supporting a link between theXIRP1variants and embryonic limb patterning. The heterozygous haplotype was highly associated with the present polydactylous phenotypes due to dominant mode of inheritance with an incomplete penetrance in Holstein cattle.
conLSH: Context based Locality Sensitive Hashing for Mapping of noisy SMRT Reads
Authors: Chakraborty Angana , Bandyopadhyay Sanghamitra 
Published: March 2019

ABSTRACTSingle Molecule Real-Time (SMRT) sequencing is a recent advancement of Next Gen technology developed by Pacific Bio (PacBio). It comes with an explosion of long and noisy reads demanding cutting edge research to get most out of it. To deal with the high error probability of SMRT data, a novel contextual Locality Sensitive Hashing (conLSH) based algorithm is proposed in this article, which can effectively align the noisy SMRT reads to the reference genome. Here, sequences are hashed together based not only on their closeness, but also on similarity of context. The algorithm has space requirement, where n is the number of sequences in the corpus and ρ is a constant. The indexing time and querying time are bounded by and respectively, where P2 > 0, is a probability value. This algorithm is particularly useful for retrieving similar sequences, a widely used task in biology. The proposed conLSH based aligner is compared with rHAT, popularly used for aligning SMRT reads, and is found to comprehensively beat it in speed as well as in memory requirements. In particular, it takes approximately 24.2% less processing time, while saving about 70.3% in peak memory requirement for H.sapiens PacBio dataset.
Bcl-xL dynamics and cancer-associated mutations under the lens of protein structure network and biomolecular simulations
Authors: Sora Valentina , Papaleo Elena 
Published: March 2019

AbstractUnderstanding the finely orchestrated interactions leading to or preventing programmed cell death (apoptosis) is of utmost importance in cancer research since the failure of these systems could eventually lead to the onset of the disease. In this regard, the maintenance of a delicate balance between promoters and inhibitors of mitochondrial apoptosis is crucial, as demonstrated by the interplay among the Bcl-2 family members. Particularly, Bcl-xL is a target of interest due to its forefront role of its dysfunctions in cancer development. Bcl-xL prevents apoptosis by binding both the pro-apoptotic BH3-only proteins, as PUMA, and noncanonical partners such as p53 at different sites. An allosteric communication between the BH3-only proteins binding pocket and the p53 binding site has been postulated and supported by NMR and other biophysical data, mediating the release of p53 from Bcl-xL upon PUMA binding. The molecular details, especially at the residue level, of this mechanism remain unclear. In this work, we investigated the distal communication between these two sites in both Bcl-xL in its free state and bound to PUMA, and we evaluated how missense mutations of Bcl-xL found in cancer samples might impair the communication and thus the allosteric mechanism. We employed all-atom explicit solvent microsecond molecular dynamics simulations analyzed through a Protein Structure Network approach and integrated with calculations of changes in free energies upon cancer-related mutations identified by genomics studies. We found a subset of candidate residues responsible for both maintaining protein stability and for conveying structural information between the two binding sites and hypothesized possible communication routes between specific residues at both sites.