bluuebunny/crossref_metadata_2025_split huggingface dataset:
Educators in Search of an Anthem: Standardizing “The Star-Spangled Banner” During the First World War
Authors: WARFIELD PATRICK 
Published: July 2018

AbstractIn the spring of 1917 several of the most prominent musicians in the United States, including the bandleader John Philip Sousa, the orchestral conductor Walter Damrosch, and the scholar Oscar Sonneck, joined together in a fruitless effort to establish a standardized version of “The Star-Spangled Banner.” Most histories of the song note this effort but fail to recognize that the impetus for it came from the music division of the National Education Association and reflected a Progressive Era faith in the efficiencies of business, which could be manifest through the mass singing of schoolchildren. This paper examines the standardization of “The Star-Spangled Banner” in light of first world war political concerns, as well as broader cultural trends. It also explains the reasons for the effort's failure and shows how the period around the first world war set up many of the struggles that inform the national anthem even today.
Simulations of Vertical Water Vapor Transport for TC Ingrid (2013)
Authors: Allison Thomas , Fuelberg Henry , Heath Nicholas 
Published: July 2018

AbstractVertical water vapor transport is examined for tropical cyclone (TC) Ingrid (2013), a Category 1 storm in the Gulf of Mexico that contained deep convection, including numerous overshooting tops. The study employs high‐resolution numerical simulations using the Weather Research and Forecasting model with a smallest grid spacing of 1.33 km and a model top of 10 hPa (~31 km) that extends into the lower stratosphere. Area‐averaged values of vertical motion, water vapor content, its temporal change, and vertical water vapor transport are presented for Ingrid as a whole and for individual regions of the storm. Results show that area‐averaged hydration in the layer ~1–14.5 km between the start and end of the simulation (4.75 days) is due to upward convective transport. Observed overshooting convection is simulated, revealing intense vertical motions that transport large quantities of vapor and ice to the lower stratosphere. Greatest dehydration occurs in the upper troposphere and lower stratosphere between 14.5 and 17.5 km. This layer is found to be supersaturated with respect to ice. This apparently caused water vapor to deposit on the convectively lofted ice, allowing it to then grow and sediment out of the layer, thus producing dehydration. Conversely, hydration occurred between 17.5 and 21 km due in part to that layer being subsaturated with respect to ice. Overall, the study highlights the important role TCs and their embedded overshooting convection play in transporting water vapor to the upper troposphere and lower stratosphere.
Convergent evolution of unusual complex I homologs with increased proton pumping capacity: energetic and ecological implications
Authors: Chadwick Grayson L , Hemp James , Fischer Woodward W , Orphan Victoria J 
Published: July 2018

Abstract
Respiratory complex I is part of a large family of homologous enzymes that carry out the transfer of electrons between soluble cytoplasmic electron carriers and membrane-bound electron carriers. These complexes are vital bioenergetic enzymes that serve as the entry points into electron transport chains for a wide variety of microbial metabolisms, and electron transfer is coupled to proton translocation. The core complex of this enzyme is made up of 11 protein subunits, with three major proton pumping subunits. Here, we document a large number of modified complex I gene cassettes found in genome sequences from diverse cultured bacteria, shotgun metagenomics, and environmentally derived archaeal fosmids all of which encode a fourth proton pumping subunit. The incorporation of this extra subunit into a functional protein complex is supported by large amino acid insertions in the amphipathic helix that runs the length of the protein complex. Phylogenetic analyses reveal that these modified complexes appear to have arisen independently multiple times in a remarkable case of convergent molecular evolution. From an energetic perspective, we hypothesize that this modification on the canonical complex I architecture allows for the translocation of a fifth proton per reaction cycle—the physiological utility of this modified complex is discussed.
Diagnosis of major depressive disorder based on changes in multiple plasma neurotransmitters: a targeted metabolomics study
Authors: Pan Jun-Xi , Xia Jin-Jun , Deng Feng-Li , Liang Wei-Wei , Wu Jing , Yin Bang-Min , Dong Mei-Xue , Chen Jian-Jun , Ye Fei , Wang Hai-Yang , Zheng Peng , Xie Peng 
Published: July 2018

AbstractMajor depressive disorder (MDD) is a debilitating psychiatric illness. However, there is currently no objective laboratory-based diagnostic tests for this disorder. Although, perturbations in multiple neurotransmitter systems have been implicated in MDD, the biochemical changes underlying the disorder remain unclear, and a comprehensive global evaluation of neurotransmitters in MDD has not yet been performed. Here, using a GC-MS coupled with LC-MS/MS-based targeted metabolomics approach, we simultaneously quantified the levels of 19 plasma metabolites involved in GABAergic, catecholaminergic, and serotonergic neurotransmitter systems in 50 first-episode, antidepressant drug-naïve MDD subjects and 50 healthy controls to identify potential metabolite biomarkers for MDD (training set). Moreover, an independent sample cohort comprising 49 MDD patients, 30 bipolar disorder (BD) patients and 40 healthy controls (testing set) was further used to validate diagnostic generalizability and specificity of these candidate biomarkers. Among the 19 plasma neurotransmitter metabolites examined, nine were significantly changed in MDD subjects. These metabolites were mainly involved in GABAergic, catecholaminergic and serotonergic systems. The GABAergic and catecholaminergic had better diagnostic value than serotonergic pathway. A panel of four candidate plasma metabolite biomarkers (GABA, dopamine, tyramine, kynurenine) could distinguish MDD subjects from health controls with an AUC of 0.968 and 0.953 in the training and testing set, respectively. Furthermore, this panel distinguished MDD subjects from BD subjects with high accuracy. This study is the first to globally evaluate multiple neurotransmitters in MDD plasma. The altered plasma neurotransmitter metabolite profile has potential differential diagnostic value for MDD.
The anti-asthmatic drug, montelukast, modifies the neurogenic potential in the young healthy and irradiated brain
Authors: Eriksson Yohanna , Boström Martina , Sandelius Åsa , Blennow Kaj , Zetterberg Henrik , Kuhn Georg , Kalm Marie 
Published: July 2018

AbstractBrain tumors are the most common form of solid tumors in children. Due to the increasing number of survivors, it is of importance to prevent long-term treatment-induced side effects. Montelukast, a leukotriene receptor antagonist, may have the desired neuroprotective properties. The aim of the study was to determine whether montelukast could reduce adverse effects of cranial irradiation (CIR) to the young brain. Daily injections of montelukast or vehicle was given to young mice for 4 or 14 days in combination with CIR or under normal conditions. Montelukast treatment for 4 days protected against cell death with 90% more cell death in the vehicle group compared to the montelukast group 24 h after CIR. It also resulted in less microglia activation 6 h after CIR, where montelukast lowered the levels of CD68 compared to the vehicle groups. Interestingly, the animals that received montelukast for 14 days had 50% less proliferating cells in the hippocampus irrespective of receiving CIR or not. Further, the total number of neurons in the granule cell layer was altered during the sub-acute phase. The number of neurons was decreased by montelukast treatment in control animals (15%), but the opposite was seen after CIR, where montelukast treatment increased the number of neurons (15%). The results show beneficial effects by montelukast treatment after CIR in some investigated parameters during both the acute phase and with longer drug treatment. However, it also resulted in lower proliferation in the hippocampus under normal conditions, indicating that the effects of montelukast can be either beneficial or unfavorable, depending on the circumstances.
Oncogenic addiction to high 26S proteasome level
Authors: Tsvetkov Peter , Adler Julia , Myers Nadav , Biran Assaf , Reuven Nina , Shaul Yosef 
Published: July 2018

AbstractProteasomes are large intracellular complexes responsible for the degradation of cellular proteins. The altered protein homeostasis of cancer cells results in increased dependency on proteasome function. The cellular proteasome composition comprises the 20S catalytic complex that is frequently capped with the 19S regulatory particle in forming the 26S proteasome. Proteasome inhibitors target the catalytic barrel (20S) and thus this inhibition does not allow the deconvolution of the distinct roles of 20S versus 26S proteasomes in cancer progression. We examined the degree of dependency of cancer cells specifically to the level of the 26S proteasome complex. Oncogenic transformation of human and mouse immortalized cells with mutant Ras induced a strong posttranscriptional increase of the 26S proteasome subunits, giving rise to high 26S complex levels. Depletion of a single subunit of the 19S RP was sufficient to reduce the 26S proteasome level and lower the cellular 26S/20S ratio. Under this condition the viability of the Ras-transformed MCF10A cells was severely compromised. This observation led us to hypothesize that cancer cell survival is dependent on maximal utilization of its 26S proteasomes. We validated this possibility in a large number of cancer cell lines and found that partial reduction of the 26S proteasome level impairs viability in all cancer cells examined and was not correlated with cell doubling time or reduction efficiency. Interstingly, normal human fibroblasts are refractory to the same type of 26S proteasome reduction. The suppression of 26S proteasomes in cancer cells activated the UPR and caspase-3 and cells stained positive with Annexin V. In addition, suppression of the 26S proteasome resulted in cellular proteasome redistribution, cytoplasm shrinkage, and nuclear deformation, the hallmarks of apoptosis. The observed tumor cell-specific addiction to the 26S proteasome levels sets the stage for future strategies in exploiting this dependency in cancer therapy.
TMEM106B drives lung cancer metastasis by inducing TFEB-dependent lysosome synthesis and secretion of cathepsins
Authors: Kundu Samrat T. , Grzeskowiak Caitlin L. , Fradette Jared J. , Gibson Laura A. , Rodriguez Leticia B. , Creighton Chad J. , Scott Kenneth L. , Gibbons Don L. 
Published: July 2018

AbstractMetastatic lung cancer is the leading cause of cancer-associated mortality worldwide, therefore necessitating novel approaches to identify specific genetic drivers for lung cancer progression and metastasis. We recently performed an in vivo gain-of-function genetic screen to identify driver genes of lung cancer metastasis. In the study reported here, we identify TMEM106B as a primary robust driver of lung cancer metastasis. Ectopic expression of TMEM106B could significantly promote the synthesis of enlarged vesicular lysosomes that are laden with elevated levels of active cathepsins. In a TFEB-dependent manner, TMEM106B could modulate the expression of lysosomal genes of the coordinated lysosomal expression and regulation (CLEAR) pathway in lung cancer cells and patient samples. We also demonstrate that TMEM106B-induced lysosomes undergo calcium-dependent exocytosis, thereby releasing active lysosomal cathepsins necessary for TMEM106B-mediated cancer cell invasion and metastasis in vivo, which could be therapeutically prevented by pharmacological inhibition of cathepsins. Further, in TCGA LUAD data sets, 19% of patients show elevated expression of TMEM106B, which predicts for poor disease-free and overall-survival.
Microscale ecology regulates particulate organic matter turnover in model marine microbial communities
Authors: Enke Tim N. , Leventhal Gabriel E. , Metzger Matthew , Saavedra José T. , Cordero Otto X. 
Published: July 2018

AbstractThe degradation of particulate organic matter in the ocean is a central process in the global carbon cycle, the mode and tempo of which is determined by the bacterial communities that assemble on particle surfaces. Here, we find that the capacity of communities to degrade particles is highly dependent on community composition using a collection of marine bacteria cultured from different stages of succession on chitin microparticles. Different particle degrading taxa display characteristic particle half-lives that differ by ~170 h, comparable to the residence time of particles in the ocean’s mixed layer. Particle half-lives are in general longer in multispecies communities, where the growth of obligate cross-feeders hinders the ability of degraders to colonize and consume particles in a dose dependent manner. Our results suggest that the microscale community ecology of bacteria on particle surfaces can impact the rates of carbon turnover in the ocean.
Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b
Authors: Cho Sunglim , Lee Hyang-Mi , Yu I-Shing , Choi Youn Soo , Huang Hsi-Yuan , Hashemifar Somaye Sadat , Lin Ling-Li , Chen Mei-Chi , Afanasiev Nikita D. , Khan Aly Azeem , Lin Shu-Wha , Rudensky Alexander Y. , Crotty Shane , Lu Li-Fan 
Published: July 2018

AbstractReciprocal interactions between B and follicular T helper (Tfh) cells orchestrate the germinal center (GC) reaction, a hallmark of humoral immunity. Abnormal GC responses could lead to the production of pathogenic autoantibodies and the development of autoimmunity. Here we show that miR-146a controls GC responses by targeting multiple CD40 signaling pathway components in B cells; by contrast, loss of miR-146a in T cells does not alter humoral responses. However, specific deletion of both miR-146a and its paralog, miR-146b, in T cells increases Tfh cell numbers and enhanced GC reactions. Thus, our data reveal differential cell-intrinsic regulations of GC B and Tfh cells by miR-146a and miR-146b. Together, members of the miR-146 family serve as crucial molecular brakes to coordinately control GC reactions to generate protective humoral responses without eliciting unwanted autoimmunity.
Unique subgingival microbiota associated with periodontitis in cirrhosis patients
Authors: Jensen Anders , Ladegaard Grønkjær Lea , Holmstrup Palle , Vilstrup Hendrik , Kilian Mogens 
Published: July 2018

AbstractLiver cirrhosis is a severe disease with major impact on the overall health of the patient including poor oral health. Lately, there has been increasing focus on oral diseases as cirrhosis-related complications due to the potential impact on systemic health and ultimately mortality. Periodontitis is one of the most common oral diseases in cirrhosis patients. However, no studies have investigated the composition of the subgingival microbiome in patients suffering from periodontitis and liver cirrhosis. We analysed the subgingival microbiome in 21 patients with periodontitis and cirrhosis using long-reads Illumina sequencing. The subgingival microbiota was dominated by bacteria belonging to the Firmicutes phylum and to a lesser extend the Actinobacteria and Bacteroidetes phyla. Bacteria usually considered periodontal pathogens, like Porhyromonas ginigivalis, Tannerella forsythia, Treponema denticola, generally showed low abundancy. Comparing the microbiota in our patients with that of periodontitis patients and healthy controls of three other studies revealed that the periodontitis-associated subgingival microbiota in cirrhosis patients is composed of a unique microbiota of bacteria not normally associated with periodontitis. We hypothesise that periodontitis in cirrhosis patients is a consequence of dysbiosis due to a compromised immune system that renders commensal bacteria pathogenic.
Identification and expression characterization of the Phloem Protein 2 (PP2) genes in ramie (Boehmeria nivea L. Gaudich)
Authors: Guo Pingan , Zheng Yancheng , Peng Dingxiang , Liu Lijun , Dai Lunjin , Chen Cong , Wang Bo 
Published: July 2018

AbstractPhloem protein 2 (PP2) is one of the most abundant and enigmatic proteins in sieve elements and companion cells, which play important roles in the maintenance of morphology, photoassimilate transportation and wound protection in higher plants, but to date, no PP2 (BnPP2) genes had been identified in ramie. Here, a total of 15 full-length BnPP2 genes were identified. These BnPP2 genes exhibited different responses to abiotic stresses. Interestingly, the BnPP2 genes are more sensitive to insect pests than to other stresses. A study of the BnPP2-15 promoter revealed that pBnPP2-15 could drive specific GUS expression in the petiole, root and stamen and could also be induced by mechanical wounding and aphid infection in transgenic Arabidopsis lines. The subcellular localization of six BnPP2 proteins showed that GFP-BnPP2-1, GFP-BnPP2-6, GFP-BnPP2-7, GFP-BnPP2-9, GFP-BnPP2-11 and GFP-BnPP2-12 were predominantly located in the cytoplasm. These results provide useful information elucidating the functions of BnPP2 genes in ramie.
Low total and free triiodothyronine levels are associated with insulin resistance in non-diabetic individuals
Authors: Wang Chih-Yuan , Yu Tse-Ya , Shih Shyang-Rong , Huang Kuo-Chin , Chang Tien-Chun 
Published: July 2018

AbstractThis study examined associations of thyroid hormone levels and insulin resistance (IR) in non-diabetic individuals. Using a cross-sectional design, 2007–2008 data from the National Health and Nutrition Examination Survey (NHANES) were analyzed. NHANES participants ≥20 years of age with complete data of interest were included. The homeostatic model assessment (HOMA) was used to quantify IR, and treated as a continuous variable. Self-reported diabetes or a fasting glucose ≥7 mmol/L were used as criteria to exclude diabetic subjects. Race, liver function, obesity, hypertension, dyslipidemia, smoking, physical activity, vigorous recreational activity, 2-hour glucose, hemoglobin A1C (HbA1C), high-density lipoprotein, triglyceride, vitamin D and C-reactive protein were covariates significantly associated with HOMA-IR. A total of 1,560 non-diabetic subjects were included in the analysis. When adjusted for all factors significant in the univariate analysis (race, liver function, obesity, hypertension, dyslipidemia, smoking, physical activity, vigorous recreational activity, 2-hour glucose, HbA1C, high-density lipoprotein, triglyceride, vitamin D, and CRP) low total triiodothyronine (TT3) and low free T3 (FT3) were significantly associated with decreased HOMA-IR (adjusted coefficient = −0.486, 95% confidence interval [CI]: −0.936, −0.036; adjusted coefficient = −1.151, 95% CI: −1.952, −0.350, respectively). Insulin resistance is associated with low thyroid hormone levels in non-diabetic individuals.
Predicting El Niño in 2014 and 2015
Authors: Ineson Sarah , Balmaseda Magdalena A. , Davey Michael K. , Decremer Damien , Dunstone Nick J. , Gordon Margaret , Ren Hong-Li , Scaife Adam A. , Weisheimer Antje 
Published: July 2018

AbstractEarly in 2014 several forecast systems were suggesting a strong 1997/98-like El Niño event for the following northern hemisphere winter 2014/15. However the eventual outcome was a modest warming. In contrast, winter 2015/16 saw one of the strongest El Niño events on record. Here we assess the ability of two operational seasonal prediction systems to forecast these events, using the forecast ensembles to try to understand the reasons underlying the very different development and outcomes for these two years. We test three hypotheses. First we find that the continuation of neutral ENSO conditions in 2014 is associated with the maintenance of the observed cold southeast Pacific sea surface temperature anomaly; secondly that, in our forecasts at least, warm west equatorial Pacific sea surface temperature anomalies do not appear to hinder El Niño development; and finally that stronger westerly wind burst activity in 2015 compared to 2014 is a key difference between the two years. Interestingly, in these years at least, this interannual variability in wind burst activity is predictable. ECMWF System 4 tends to produce more westerly wind bursts than Met Office GloSea5 and this likely contributes to the larger SST anomalies predicted in this model in both years.
The Chemical and Products Database, a resource for exposure-relevant data on chemicals in consumer products
Authors: Dionisio Kathie L. , Phillips Katherine , Price Paul S. , Grulke Christopher M. , Williams Antony , Biryol Derya , Hong Tao , Isaacs Kristin K. 
Published: July 2018

AbstractQuantitative data on product chemical composition is a necessary parameter for characterizing near-field exposure. This data set comprises reported and predicted information on more than 75,000 chemicals and more than 15,000 consumer products. The data’s primary intended use is for exposure, risk, and safety assessments. The data set includes specific products with quantitative or qualitative ingredient information, which has been publicly disclosed through material safety data sheets (MSDS) and ingredient lists. A single product category from a refined and harmonized set of categories has been assigned to each product. The data set also contains information on the functional role of chemicals in products, which can inform predictions of the concentrations in which they occur. These data will be useful to exposure and risk assessors evaluating chemical and product safety.
A cluster of Zika virus infection in a Chinese tour group returning from Fiji and Samoa
Authors: Sun Jimin , Fu Tao , Mao Haiyan , Wang Zhen , Pan Junhang , Rutherford Shannon , Ren Jiangping , Dong Xuanjun , Chen Yin , Zhu Zhihong , Qi Xiaohua , Gong Zhenyu , Liu Qiyong , Yu Hongjie , Zhu Liebo , Chen Wenxian , Chen Zhiping , Zhang Yanjun , Chen Enfu 
Published: July 2018

AbstractZika virus is currently causing extensive outbreaks in a number of countries in South and Central America and the Caribbean and has been associated with foetal abnormalities. We report an outbreak of Zika virus infection in a Chinese tour-group returning from a nine day holiday in Fiji and Samoa. The index case was a 38-year old male who developed symptoms while travelling back from Fiji to Hong Kong on the 14th February, 2016. A field investigation was initiated to define the epidemiological, clinical and virological characteristics of Zika virus infection in this tour group and revealed two further symptomatic infections and one asymptomatic infection among the 33 travellers; an overall infection attack rate of 12% in these travellers. Active surveillance led to detection of Zika virus RNA in the serum of one case four days prior to onset of symptoms and detection of Zika virus in saliva from one asymptomatic infection.