bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occurrence and characterization of carbapenem‐resistant Gram‐negative bacilli: A collaborative study of antibiotic‐resistant bacteria between Indonesia and Japan
Authors: Kuntaman Kuntaman , Shigemura Katsumi , Osawa Kayo , Kitagawa Koichi , Sato Koharu , Yamada Naoki , Nishimoto Kento , Yamamichi Fukashi , Rahardjo Dadik , Hadi Usman , Mertaniasih Ni Made , Kinoshita Shohiro , Fujisawa Masato , Shirakawa Toshiro 
Published: September 2018

ObjectivesTo explore the occurrence and characterization of carbapenemase‐producing pathogens among carbapenem‐resistant Gram‐negative bacilli isolated from hospitalized patients with urinary tract infection in Indonesia.MethodsThis was a study promoted by the Japanese–Indonesian collaborative research program in the Japan Initiative for Global Research Network on Infectious Diseases. Bacterial pathogens were prospectively isolated from urine specimens of hospitalized urinary tract infection patients at Dr. Soetomo Hospital (Surabaya, Indonesia). All Gram‐negative bacteria resistant to third‐generation cephalosporin or carbapenem were included in this study. Carbapenemase genes were investigated for phenotype and genotype.ResultsIn total, 1082 Gram‐negative bacilli were isolated, of which 116 strains were resistant to imipenem or meropenem (carbapenem‐resistant Gram‐negative bacilli), and 22 strains were carbapenemase‐producing Gram‐negative bacilli. Carbapenemase‐producing Gram‐negative bacilli consisted of Acinetobacter baumannii (n = 4), Pseudomonas aeruginosa (n = 4), Klebsiella pneumoniae (n = 5), Providencia rettgeri (n = 4) and five others. The carbapenemase‐producing Gram‐negative bacilli included NDM‐1 (n = 18, 81.8%, in Enterobacteriaceae and Acinetobacter spp.) and IMP‐7 (n = 4, 18.2%, all in P. aeruginosa). Among carbapenem‐resistant Gram‐negative bacilli, all four P. aeruginosa were sensitive to colistin, and all six Acinetobacter spp. were sensitive to minocycline, colistin and tigecycline. Of those patients harboring carbapenemase‐producing Gram‐negative bacilli, 12 (54.5%) were seriously ill at the time of admission, with longer hospital stays and three deaths (13.6% mortality rate).ConclusionsUrinary tract infection‐causing carbapenem‐resistant Gram‐negative bacilli are widely disseminated in Indonesia. The NDM‐1 phenotype seems to be dominant, and it can be treated with colistin and tigecycline in most cases. Most patients harboring carbapenemase‐producing Gram‐negative bacilli are seriously ill, have a bad prognosis, with a longer hospital stay and a significant mortality rate.
Acute kidney injury and intermediate‐term renal function after clampless partial nephrectomy
Authors: Kawamura Naoko , Yokoyama Minato , Tanaka Hajime , Nakayama Takayuki , Yasuda Yosuke , Kijima Toshiki , Yoshida Soichiro , Ishioka Junichiro , Matsuoka Yoh , Saito Kazutaka , Kihara Kazunori , Fujii Yasuhisa 
Published: September 2018

ObjectivesTo evaluate the incidence and predictors of acute kidney injury after clampless partial nephrectomy, and its impact on intermediate‐term renal function.MethodsThe incidence and severity of acute kidney injury were assessed for 262 patients undergoing clampless partial nephrectomy between 2010 and 2015. The association between perioperative covariates and acute kidney injury was evaluated using multivariate logistic regression analysis. An annual change in estimated glomerular filtration rate from 1 year after surgery was calculated according to the presence or absence of acute kidney injury. An impact of acute kidney injury on postoperative renal impairment, defined as a ≥25% estimated glomerular filtration rate decrease, was evaluated.ResultsOverall, 21 (8.0%) patients experienced grade 1 acute kidney injury after clampless partial nephrectomy, and grade ≥2 acute kidney injury was not observed. High tumor complexity was the only independent predictor of acute kidney injury. Estimated glomerular filtration rate in patients with acute kidney injury improved within 1 year, and annual estimated glomerular filtration rate changes were similar among patients with or without acute kidney injury. Ultimately, 13 (5.0%) patients showed postoperative renal impairment during the median follow‐up period of 37 months. Advanced age and diabetes mellitus were independent risk factors for renal impairment, but acute kidney injury was not.ConclusionsThe incidence and severity of acute kidney injury after clampless partial nephrectomy are low. Low‐grade acute kidney injury after clampless partial nephrectomy does not seem to affect intermediate‐term renal function.
Interactions between rheumatoid arthritis synovial fibroblast migration and endothelial cells
Authors: Zimmermann‐Geller Birgit , Köppert Sina , Kesel Nina , Hasseli Rebecca , Ullrich Sebastian , Lefèvre Stephanie , Frommer Klaus , Gehrke Thorsten , Schönburg Markus , Rehart Stephan , Schumacher Udo , Müller‐Ladner Ulf , Neumann Elena 
Published: September 2018

AbstractLeukocytes travel within the circulation and enter connective tissues by interactions with endothelium of postcapillary venules mediated by cell adhesion molecules, summarized as the leukocyte adhesion cascade. In the severe combined immunodeficient (SCID) mouse model, rheumatoid arthritis (RA) synovial fibroblasts (SF) migrated to distant cartilage through the vasculature. Therefore, RASF adhesion toward endothelial cells (EC) and E‐ and P‐selectins were analyzed. Cell‐to‐cell binding assays between SF and EC were performed. Interactions of SF with tumor necrosis factor α (TNFα)‐activated EC or selectins were analyzed in flow adhesion assays. Immunohistochemistry for E‐selectin ligand CD15s was performed. CD15s induction in RASF by human serum or media was evaluated. Wild‐type and E‐/‐/P‐/‐Selectin‐SCID mice were used for inverse‐wrap surgery. After laser‐mediated microdissection, real‐time PCR for E‐/P‐selectin/vascular cell adhesion molecule 1 was performed. Adhesion between SF/EC under static conditions was highest in Roswell Park Memorial Institute‐cultured RASF to TNFαα‐activated human umbilical vein endothelial cells (2.25‐fold) and RASF adhesion was higher toward venous than arterial EC (Dulbecco's modified eagle medium P = 0.0419, RPMI P = 0.0119). In flow chamber assays, RASF adhesion to E‐selectin was higher than to P‐selectin (e.g. 0.9 dyn cm−2 P = 0.0001). Osteoarthritis synovial fibroblasts showed lower rolling/adhesion properties (e.g. 0.5 dyn cm−2, P = 0.0010). RASF adhesion to TNFαα‐activated EC was increased (e.g. 0.9 dyn cm−2, P = 0.0061). CD15s induction in RASF was strongest in RA serum. Vimentin/CD15s double‐positive cells were detectable. In E‐/P‐selectin‐deficient mice, contralateral invasion was reduced (P = 0.023). E‐ and P‐selectin, and vascular cell adhesion molecule 1 expression in EC of implants was confirmed. Our data indicate that the milieu within vessels induces CD15s which enables RASF to interact with E‐selectin/EC under flow. Therefore, RASF may migrate to distant sites and leave the vasculature similarly to leukocytes.
Hydrochlorothiazide vs chlorthalidone, indapamide, and potassium‐sparing/hydrochlorothiazide diuretics for reducing left ventricular hypertrophy: A systematic review and meta‐analysis
Authors: Roush George C. , Abdelfattah Ramy , Song Steven , Ernst Michael E. , Sica Domenic A. , Kostis John B. 
Published: September 2018

Left ventricular hypertrophy develops in 36%‐41% of hypertensive patients and independently predicts cardiovascular events and total mortality. Moreover, drug‐induced reduction in left ventricular mass (LVM) correlates with improved prognosis. The optimal thiazide‐type diuretic for reducing LVM is unknown. Evidence regarding potency, cardiovascular events, sodium, and potassium suggested the hypothesis that “CHIP” diuretics (CHlorthalidone, Indapamide, and Potassium‐sparing diuretic/hydrochlorothiazide [PSD/HCTZ]) would reduce LVM more than HCTZ. Systematic searches of five databases were conducted. Among the 38 randomized trials, a 1% reduction in systolic blood pressure (SBP) predicted a 1% reduction in LVM, P = 0.00001. CHIP‐HCTZ differences in reducing LVM differed across trials (ie, heterogeneity), making interpretation uncertain. However, among the 28 double‐blind trials, heterogeneity was undetectable, and HCTZ reduced LVM (percent reduction [95% CI]) by −7.3 (−10.4, −4.2), P < 0.0001. CHIP diuretics surpassed HCTZ in reducing LVM: chlorthalidone −8.2 (−14.7, −1.6), P = 0.015; indapamide −7.5 (−12.7, −2.3), P = 0.005; and all CHIP diuretics combined −7.7 (−12.2, −3.1), P < 0.001. The comparison of PSD/HCTZ with HCTZ had low statistical power but favored PSD/HCTZ: −6.0 (−14.1, +2.1), P = 0.149. Thus, compared to HCTZ, CHIP diuretics had twice the effect on LVM. CHIP diuretics did not surpass HCTZ in reducing systolic or diastolic blood pressure: −0.3 (−5.0, +4.3) and −1.6 (−5.6, +2.4), respectively. The strength of evidence that CHIP diuretics surpass HCTZ for reducing LVM was high (GRADE criteria). In conclusion, these novel results have demonstrated that CHIP diuretics reduce LVM 2‐fold more than HCTZ among hypertensive patients. Although generally related to LVM, blood pressure fails to explain the superiority of CHIP diuretics for reducing LVM.
12(S)‐hydroxyeicosatetraenoic acid impairs vascular endothelial permeability by altering adherens junction phosphorylation levels and affecting the binding and dissociation of its components in high glucose‐induced vascular injury
Authors: Wang Xiaofang , Gao Lu , Xiao Lili , Yang Lulu , Li Wenshu , Liu Gangqiong , Chen Linlin , Zhang Jinying 
Published: September 2018

AbstractAims/IntroductionDiabetes is an important risk factor for atherosclerotic disease. The initiating factor of atherosclerosis is local endothelial cell injury. The arachidonic acid metabolite, 12(S)‐hydroxyeicosatetraenoic acid (12[S]‐HETE), might be involved in this process. In recent years, some studies have discussed the effect of 12(S)‐HETE on vascular endothelial cell function. In the present study, we investigated the effect of 12(S)‐HETE on vascular endothelial cell function in high‐glucose conditions and the mechanisms involved.Materials and MethodsHuman umbilical vein endothelial cells were cultured in conventional M199 medium and high‐glucose M199 medium. Human umbilical vein endothelial cells were stimulated with 12(S)‐HETE and cinnamyl‐3,4‐dihydroxy‐α‐cyanocinnamate (a 12/15‐lipoxygenases inhibitor). A type 1 diabetes mellitus model was established in C57BL/6 or 12/15‐lipoxygenases knockout mice with streptozotocin. Aortic tissue was harvested for subsequent testing. The transmembrane transport of dextran and human acute monocytic leukaemia cell line (THP‐1) cells was measured. The adherens junction protein, IkBα, nuclear factor kappa Bp65 (P65), intercellular adhesion molecule 1 and vascular cell adhesion protein 1 expression and phosphorylation, and the binding/dissociation of endothelial cell components were observed.ResultsTransendothelial migration of dextran and THP‐1 cells was significantly increased by stimulation of human umbilical vein endothelial cell monolayers with high glucose and 12(S)‐HETE (P < 0.05). High glucose and 12(S)‐HETE altered the vascular endothelial cadherin and β‐catenin phosphorylation level, and promoted the dissociation of β‐catenin and vascular endothelial cadherin. Expression levels of P‐Ikbα, P‐P65, intercellular adhesion molecule 1 and vascular cell adhesion protein 1 were elevated in high glucose and 12(S)‐HETE treated cells and diabetic mice compared with controls (P < 0.05).ConclusionsThe lipoxygenases metabolite, 12(S)‐HETE, can impair vascular endothelial permeability by altering adherens junction phosphorylation levels, and affecting the binding and dissociation of its components in high‐glucose conditions.
DNABarcoding as useful tool to identify crop pest flea beetles of Turkey
Authors: Coral Şahin Didem , Magoga Giulia , Özdikmen Hüseyin , Montagna Matteo 
Published: September 2018

AbstractFlea beetles (Chrysomelidae: Galerucinae: Alticini), with ~8,000 species worldwide, include pest species causing substantial economic damage to crops. The generaPhyllotretaandChaetocnemainclude both pest and non‐pest species. An accurate and fast taxonomic identification approach is required for discriminating among taxa for non‐expert taxonomists; moreover, the utility of this approach spans from biodiversity conservation to the monitoring of pest species.DNAbarcoding represents a reliable and easy identification tool based on the use of shortDNAsequences. In this study, 45 newCOIsequences of 13Phyllotretaand fiveChaetocnemaspecies, representing ~30% and ~20% of the Turkish species belonging to these genera, were provided. These sequences increased by ~18% and ~25% the number of species of these genera whose sequences are available inBOLD. In order to testDNAbarcoding efficiency inPhyllotretaandChaetocnemaspecies identification, we created a data set consisting of sequences belonging to species present in the Middle East and available inBOLDplus the sequences developed in this study (36 species). The efficiency of species identification, estimated using best close match analysis (with the ad hoc calculated optimal distance threshold of 1.5%), was 99%. The overall intraspecific and interspecific mean nucleotide divergences were 1.4% and 20%, respectively. Interestingly,COIsequences ofPhyllotreta nigripesclustered into two well‐separated groups with a high value of the between‐group nucleotide distance (11.4%), which suggests the presence of cryptic species. In addition, information was provided on the crops exploited by the collected organisms and the observed damage.
The potential of novel African isolates of Phthorimaea operculella granulovirus for the control of Tuta absoluta
Authors: Ben Tiba Saoussen , Larem Andreas , Laarif Asma , Fritsch Eva , Undorf‐Spahn Karin , Abuelgasim Mohamed Samira , Ekesi Sunday , Wennmann Jörg T. , Kroschel Jürgen , Fattouch Sami , Jehle Johannes A. 
Published: September 2018

AbstractPhthorimaea operculella granulovirus (PhopGV) is infectious for larvae of different Gelechiidae insect species, including Tuta absoluta and Phthorimaea operculella. As these are major economic pests in North and sub‐Saharan Africa as well as in the Mediterranean area, the development of locally suitable biocontrol agents is essential. We have studied five isolates of PhopGV from Tunisia (Tns16, Tu1.11 and Tu2.11), Kenya (Ken13) and Yemen (Ym14) for their biological activity and the sequence polymorphism of their granulin and ecdysteroid UDP‐glucosyltransferase (egt) genes and allocated the isolates to two different egt types. Infection experiments with neonate larvae of T. absoluta and P. operculella demonstrated their pathogenicity to both host species. The isolate PhopGV Tu1.11 was the most virulent one for T. absoluta but had a relatively low infectivity to two P. operculella populations originating from Italy and Tunisia.
Long‐term risk assessment on noneffective and effective toxic doses of imidacloprid to honeybee workers
Authors: Zhu Yu Cheng , Yao Jianxiu , Adamczyk John 
Published: September 2018

AbstractImidacloprid is the most widely used insecticide in agriculture. Its impact on honeybees has received worldwide attention. Foliar sprays are commonly and frequently used for piercing insect control, particularly on cotton in southern USA. To simulate field exposures of formulated imidacloprid (Advise® 2Fl), we used a modified spray tower to treat honeybee workers and monitored five enzyme activities and survival for up to 52 days. Results indicated that spray treatments twice a week for 52 days with 0.001 and 1 mg/L and once a week for three weeks with 4.3 mg/L Advise showed no adverse effect on bee survival, where imidacloprid‐treated bees could live as long as untreated bees. Concentration ≥80 mg/L significantly reduced bee survival, and substantial number of bees continued to die after 48‐hr of post‐treatment period which was commonly used for measuring insecticide toxicity. The body weight of imidacloprid‐treated bees (at LC20 and LC50) was also significantly reduced. Enzymatic data showed that activities of detoxification enzymes esterase and glutathione S‐transferase (GST), insecticide‐target enzyme acetylcholinesterase (AChE) and honey enzyme invertase in imidacloprid‐treated survivors were mostly similar to those found in untreated bees. The immunity‐related phenoloxidase (PO) activity in imidacloprid‐treated survivors was also mostly similar to that of untreated control, but higher PO activity was detected in bees treated with higher concentrations for 3 weeks. By using both bioassays and enzymatic assays, this study revealed long‐term noneffective and effective concentrations of imidacloprid that may be useful for accurate assessment of toxicity risk of neonicotinoids to bees.
Zirconia dental implants: A clinical and radiographic evaluation
Authors: Rodriguez Angel E. , Monzavi Mona , Yokoyama Chester L. , Nowzari Hessam 
Published: September 2018

AbstractObjectiveLimited clinical evidence has been reported in dental literature regarding zirconia dental implants. The aim of the present retrospective consecutive case series was to evaluate the clinical outcome of zirconia dental implants with 1 to 5 years of follow‐up.Clinical considerationsThe clinical outcome of 24 implants in 12 consecutive patients (male 5, female 7) with a mean age of 55 years (range 27‐86) is reported. Radiographic crestal bone level was assessed by the use of “ImageJ” software program. Gingival and plaque indices were recorded at baseline and latest follow‐up.ConclusionThe overall success rate of zirconia implants was 92%. Within the limitations of the present clinical evaluation, zirconia implants provided excellent clinical results and esthetic outcomes. A mean periimplant bone loss of 0.3 mm was measured in 33.3% of the implants and 66.7% were not affected by radiographic detectable periimplant bone loss. Two implants in two patients failed. Low gingival and plaque indices were predominant values for both interim and final restorations. The apparent less affinity to plaque accumulation may favor soft tissue health around zirconia dental implants and decrease the risk of inflammation or infection. Zirconia dental implant merits further investigation.Clinical significanceZirconia dental implants are emerging as an option in clinical practice of implant dentistry providing stable clinical results and esthetic outcomes.
Association of Hospitalization, Critical Illness, and Infection with Brain Structure in Older Adults
Authors: Walker Keenan A. , Gottesman Rebecca F. , Wu Aozhou , Knopman David S. , Mosley Thomas H. , Alonso Alvaro , Kucharska‐Newton Anna , Brown Charles H. 
Published: September 2018

ObjectivesTo examine the association between hospitalization, critical illness, and infection occurring during middle‐ and late‐life and structural brain abnormalities in older adults.DesignProspective cohort study.SettingAtherosclerosis Risk in Communities (ARIC) Study.ParticipantsA community sample of adults who were 44 to 66 years of age at study baseline.MeasurementsActive surveillance of local hospitals and annual participant contact were used to gather hospitalization information (including International Classification of Diseases, Ninth Revision, codes) on all participants over a 24‐year surveillance period. Subsequently, a subset of participants underwent 3‐Tesla brain magnetic resonance imaging (MRI) to quantify total and regional brain volumes, white matter hyperintensity (WMH) volume, and white matter microstructural integrity (fractional anisotropy (FA) and mean diffusivity (MD) as measured using diffusion tensor imaging (DTI)).ResultsOf the 1,689 participants included (mean age at MRI 76±5), 72% were hospitalized, 14% had a major infection, and 4% had a critical illness during the surveillance period. Using covariate‐adjusted regression, hospitalization was associated with 0.12–standard deviation (SD) greater WMH volume (95% confidence interval (CI)=0.00–0.24) and poorer white matter microstructural integrity (0.17‐SD lower FA, 95% CI=–0.27 to –0.06; 0.16‐SD greater MD, 95% CI=0.07–0.25) than no hospitalization. There was a dose‐dependent relationship between number of hospitalizations, smaller brain volumes, and lower white matter integrity (p‐trends ≤.048). In hospitalized participants, critical illness was associated with smaller Alzheimer's disease (AD) signature region (–1.64 cm3, 95% CI=–3.16 to –0.12); major infection was associated with smaller AD signature region (–1.28 cm3, 95% CI=–2.21 to –0.35) and larger ventricular volume (3.79 cm3, 95% CI= 0.81–6.77).ConclusionsWhereas all‐cause hospitalization was primarily associated with lower white matter integrity, critical illness and major infection were associated with smaller brain volume, particularly within regions implicated in AD.
Genomic divergence in cotton germplasm related to maturity and heterosis
Authors: He Shoupu , Sun Gaofei , Huang Longyu , Yang Daigang , Dai Panhong , Zhou Dayun , Wu Yuzhen , Ma Xiongfeng , Du Xiongming , Wei Shoujun , Peng Jun , Kuang Meng 
Published: September 2018

AbstractCommercial varieties of upland cotton (Gossypium hirsutum) have undergone extensive breeding for agronomic traits, such as fiber quality, disease resistance, and yield. Cotton breeding programs have widely used Chinese upland cotton source germplasm (CUCSG) with excellent agronomic traits. A better understanding of the genetic diversity and genomic characteristics of these accessions could accelerate the identification of desirable alleles. Here, we analyzed 10,522 high‐quality single‐nucleotide polymorphisms (SNP) with the CottonSNP63K microarray in 137 cotton accessions (including 12 hybrids of upland cotton). These data were used to investigate the genetic diversity, population structure, and genomic characteristics of each population and the contribution of these loci to heterosis. Three subgroups were identified, in agreement with their known pedigrees, geographical distributions, and times since introduction. For each group, we identified lineage‐specific genomic divergence regions, which potentially harbor key alleles that determine the characteristics of each group, such as early maturity‐related loci. Investigation of the distribution of heterozygous loci, among 12 commercial cotton hybrids, revealed a potential role for these regions in heterosis. Our study provides insight into the population structure of upland cotton germplasm. Furthermore, the overlap between lineage‐specific regions and heterozygous loci, in the high‐yield hybrids, suggests a role for these regions in cotton heterosis.