bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mycoplasma genitalium Infections With Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama
Authors: Xiao Li , Waites Ken B. , Van Der Pol Barbara , Aaron Kristal J. , Hook Edward W. , Geisler William M. 
Published: July 2018

Background

Mycoplasma genitalium (MG) is a sexually transmitted pathogen associated with inflammatory syndromes in men and women. Macrolides and fluoroquinolones are recommended MG treatments. The frequency of MG strains with macrolide resistance-associated mutations (MRMs) and quinolone resistance-associated mutations (qRMs) is increasing worldwide, however these data are sparse in populations in the United States.

Methods
We investigated the prevalence of MG infections with MRMs and qRMs and MG infection concordance within African American couples in Birmingham, AL. We used a real-time polymerase chain reaction to detect MG and identify MRMs. quinolone resistance-associated mutations were detected using traditional polymerase chain reactions amplifying regions in gyrA, gyrB, parC, and parE. The MG concordance in couples was evaluated by MG positivity and MG genotypes.

Results
Oral, anal, urine, and/or vaginal specimens were tested from 116 couples. Twenty-eight (12.1%) participants comprising 22 couples tested MG-positive (11.2% in men and 12.9% in women). Macrolide resistance-associated mutations were detected in 17 (60.7%) MG-positive participants, with gender-specific resistance rates of 69.2% for men and 53.3% for women. quinolone resistance-associated mutations were detected in 3 (11.1%) MG-positive participants, all of whom also had MRMs. By MG positivity status, 27.3% of couples were concordant. If MG strain genotypes are also considered, then concordance was 20.0%.

Conclusions
Among heterosexual African Americans with MG infection, about 60% had strains with MRMs and 11% had strains with both MRMs and qRMs, highlighting the potential for MG treatment failure to not only macrolides, but also quinolones. These findings may help to guide clinicians in MG testing and treatment decisions in the United States.
Accuracy of Three Software Applications for Breast Volume Calculations from Three-Dimensional Surface Images
Authors: Wesselius Tycho S. , Verhulst Arico C. , Vreeken Rinaldo D. , Xi Tong , Maal Thomas J. J. , Ulrich Dietmar J. O. 
Published: July 2018

Background:
Knowing breast volumes before certain surgeries helps the surgeon to obtain breast symmetry. Calculating breast volumes from three-dimensional surface images is possible with specialized software applications. However, limited data exist concerning the accuracy of such volume calculations. The purpose of this study was to investigate the accuracy of breast volume calculations performed with the 3D BreAST, 3dMD Vultus, and VECTRA software applications.

Methods:
Twenty-six subjects who underwent 44 mastectomies were enrolled. Preoperative three-dimensional surface images were acquired with a VECTRA-XT stereophotogrammetry device. Breast volumes were calculated from these images with the three software applications. The mastectomy specimens were weighed to derive their actual volume and compared with the software calculations.

Results:
For all three methods, a positive correlation between the breast volume and absolute calculation error was found (p < 0.001), but not for the errors as a percentage of the breast volume (p = 0.17, p = 0.80, and p = 0.42). The 3D BreAST, 3dMD Vultus, and VECTRA applications provided mean volume calculation errors of 21, 186, and −32 ml (p = 0.27, p < 0.001, and p = 0.14) or 2 ± 25, 48 ± 26, and −6 ± 27 percent of the breast volume (p = 0.67, p < 0.001, and p = 0.16), respectively.

Conclusions:
Despite that two applications calculated accurate mean breast volumes, all applications showed a high standard deviation in terms of the percentage of the breast volume. Therefore, the usefulness of absolute breast volume calculations from three-dimensional surface images seems limited.

CLINICAL QUESTION/LEVEL OF EVIDENCE:
Diagnostic, II.
The Orbital Oval Balance Principle: A Morphometric Clinical Analysis
Authors: Gülbitti Haydar A. , Bouman Theo K. , Marten Timothy J. , van der Lei Berend 
Published: July 2018

Background:
The aim of this study was to test the validity of the “orbital oval balance principle,” a system of analysis and guideline that is used among aestheticians, artists, and makeup artists to create and design aesthetically pleasing eyebrows for optimal upper facial appearance. According to this principle, a face is optimally attractive when the eye is centered in an “oval” defined by the lid-cheek junction and the eyebrow.

Methods:
One hundred participants were asked to rank digital morphed images of four female models with four different periorbital proportions: higher or lower lid-cheek junction versus higher or lower eyebrow position. In addition, the participants were asked to quantify seven emotions on these morphed images.

Results:
A higher lid-cheek junction was rated as significantly more attractive with a lower eyebrow position, and a lower lid-cheek junction was regarded far more attractive in combination with a higher eyebrow position. Moreover, a higher lid-cheek junction was rated as more attractive than a lower lid-cheek junction, and elevation of the lid-cheek junction improved the perception of emotions such as tiredness and sadness.

Conclusions:
This study lends support to the orbital oval balance principle that can provide important insight into facial attractiveness to surgeons undertaking procedures intended to improve and rejuvenate facial appearance. Moreover, this study has also shown not only that a high(er) lid-cheek junction is regarded as more youthful and attractive, but that it also may reduce the appearance of tiredness and sadness.
Multimonth Prescription of Antiretroviral Therapy Among Children and Adolescents: Experiences From the Baylor International Pediatric AIDS Initiative in 6 African Countries
Authors: Kim Maria H. , Wanless Richard S. , Caviness Alison Chantal , Golin Rachel , Amzel Anouk , Ahmed Saeed , Mhango Joseph , Damba David , Kayabu Angelina , Chodota Moses , Dlamini Sandile , Chidah Nodumo , Mokhali Mokhitli , Calles Nancy R. , Abrams Elaine J. 
Published: July 2018

Background:
To reach 90-90-90 targets, differentiated approaches to care are necessary. We describe the experience of delivering multimonth prescription (MMP) schedules of antiretroviral therapy (ART) to youth at centers of excellence in 6 African countries.

Methods:
We analyzed data from electronic medical records of patients aged 0–19 years started on ART. Patients were eligible to transition from monthly prescribing to MMP when clinically stable [improving CD4+, viral load (VL) suppression, or minimal HIV-associated morbidity] and ART adherent (pill count 95%–105%). Patients were classified as transitioned to MMP after 3 consecutive visits at intervals of >56 days. We used survival analysis to describe death and lost to follow-up. We described adherence and acceptable immunologic response by CD4+ using 6-month and VL suppression (<400 copies per milliliter) using 12-month intervals.

Results:
Twenty-two thousand six hundred fifty-eight patients aged 0–19 years received ART and 14,932 (66%) transitioned to MMP between 2003 and 2015. Of these 2.6% were lost to follow-up and 2.0% died. Median duration of MMP was 3.9 (interquartile range: 2.2–5.9) years. There were significant differences in survival (P < 0.0001) between age groups, worst among those younger than 1 year and 15–19 years. The frequency of favorable clinical endpoints was high throughout the first 5 years of MMP, by year ranging from 87% to 94% acceptable immunologic response, 75% to 80% adherent, and 79% to 85% VL suppression.

Conclusions:
These analyses from 6 African countries demonstrate that youth on ART who transitioned to MMP overall maintained favorable outcomes in terms of death, retention, adherence, immunosuppression, and viral suppression. These results reassure that children and adolescents, who are clinically stable and ART adherent, can do well with reduced visit frequencies and extended ART refills.
Community-Based Interventions to Reach 95-95-95 for Children and Adolescents: An Exploratory Programmatic Review From Lesotho
Authors: Amzel Anouk , Srivastava Meena , Isavwa Anthony , Sanders Jill , Tumbare Esther , Membe Ian , Mirembe Justine , Ntjabane Seema , Raliile Peter , Mohoanyane Matsitso , Ryan Victoria 
Published: July 2018

Background:
Viral suppression is the desired outcome for children and adolescents with HIV. In this article, data from districts supporting community interventions (implementation districts) were reviewed and compared with data from districts without community interventions (nonimplementation districts) to explore a potential correlation between community interventions and clinical outcomes.

Setting:
The study was based on data collected from facilities in 6 districts in Lesotho.

Methods:
Twelve-month retention, viral load coverage, and viral suppression data from patients with ART between ages 5 and 24 from facilities in both district types were collected retrospectively.

Results:
Implementation districts showed retention rates of 75%, with 5365 patients (47% of all patients on ART) having documented viral load results and 4641 (87%) being virally suppressed. Retention comparison demonstrated significantly higher rates in implementation districts (73%) as compared to (63%) in nonimplementation districts (P = 0.023). Viral load coverage and suppression comparison found that implementation district hospitals reported 632 (37% of total on ART) patients with a documented viral load, with 539 (85%) virally suppressed, whereas nonimplementation district hospitals reported 220 (31%) patients with viral load results, of whom 181 (82%) were suppressed.

Conclusions:
Overall, retention rates in the implementation districts were reasonable and were significantly better than the rates in the nonimplementation districts.
Learning Curve Under Proctorship of Pure Laparoscopic Living Donor Left Lateral Sectionectomy for Pediatric Transplantation
Authors: Broering Dieter C. , Berardi Giammauro , El Sheikh Yasser , Spagnoli Alessandra , Troisi Roberto I. 
Published: July 2018

Objective:
To evaluate the learning curve of an expert liver transplantation surgeon approaching fully laparoscopic living donor left lateral sectionectomy (L-LLS) under proctorship.

Background:
Laparoscopic liver resections necessitate a long learning curve trough a stepwise fulfillment of difficulties. L-LLS requires expertise in both living donor liver transplantation and advanced laparoscopic liver surgery. There is currently no data about the learning curve of L-LLS.

Methods:
A total of 72 pure L-LLS were included in this study. A Broken line model was used to identify the periods of the learning curve. A CUSUM analysis of the operative time was performed to evaluate improvements of outcomes with time. To evaluate the relationship between operative time and progressive number of procedures, a linear regression model was applied. A receiver operating characteristic (ROC) curve was carried out to identify the cutoff for completion of the learning curve.

Results:
Operative time decreased with the progressive increase of procedures. Two cutoffs and 3 different periods were identified: cases 1 to 22, cases 23 to 55, and cases 56 to 72. A significant decrease in blood loss and operative time was noted. The CUSUM analysis showed an increase in operative time in the first period, a stable duration in the second period, and a decrease in the last. Blood loss was significantly associated with an increase in operative time (P = 0.003). According to the ROC curve, the learning curve was completed after 25 procedures.

Conclusions:
L-LLS is a safe procedure that can be standardized and successfully taught to surgeons with large experience in donor hepatectomy through a proctored learning curve.
Illuminating gillnets to save seabirds and the potential for multi-taxa bycatch mitigation
Authors: Mangel Jeffrey C. , Wang John , Alfaro-Shigueto Joanna , Pingo Sergio , Jimenez Astrid , Carvalho Felipe , Swimmer Yonat , Godley Brendan J.  
Published: July 2018

Bycatch in net fisheries is recognized as a major source of mortality for many marine species, including seabirds. Few mitigation solutions, however, have been identified. We assessed the effectiveness of illuminating fishing nets with green light emitting diodes (LEDs) to reduce the incidental capture of seabirds. Experiments were conducted in the demersal, set gillnet fishery of Constante, Peru and compared 114 pairs of control and illuminated nets. We observed captures of a total of 45 guanay cormorants (
Phalacrocorax bougainvillii
), with 39 caught in control nets and six caught in illuminated nets. Seabird bycatch in terms of catch-per-unit-effort was significantly (
p
< 0.05) higher in control nets than in illuminated nets, representing an 85.1% decline in the cormorant bycatch rate. This study, showing that net illumination reduces seabird bycatch and previous studies showing reductions in sea turtle bycatch without reducing target catch, indicates that net illumination can be an effective multi-taxa bycatch mitigation technique. This finding has broad implications for bycatch mitigation in net fisheries given LED technology's relatively low cost, the global ubiquity of net fisheries and the current paucity of bycatch mitigation solutions.
The complex genetic architecture of shoot growth natural variation in Arabidopsis thaliana
Authors: Marchadier  , Hanemian  , Tisné  , Bach  , Bazakos  , Gilbault  , Haddadi  , Virlouvet  , Loudet  
Published: July 2018

AbstractOne of the main outcome of quantitative genetics approaches to natural variation is to reveal the genetic architecture underlying the phenotypic space. Complex genetic architectures are described as including numerous loci (or alleles) with small-effect and/or low-frequency in the populations, interactions with the genetic background, environment or age… Linkage or association mapping strategies will be more or less sensitive to this complexity, so that we still have an unclear picture of its extent. By combining high-throughput phenotyping under two environmental conditions with classical QTL mapping approaches in multiple Arabidopsis thaliana segregating populations as well as advanced near isogenic lines construction and survey, we have attempted to push back the limits of our understanding of quantitative phenotypic variation. Integrative traits such as those related to vegetative growth used in this work (highlighting either cumulative growth, growth rate or morphology) all showed complex and dynamic genetic architecture with respect to the segregating population and condition. The more resolutive our mapping approach, the more complexity we uncover, with several instances of QTLs visible in near isogenic lines but not detected with the initial QTL mapping, indicating that our phenotyping resolution was less limiting than the mapping resolution with respect to the underlying genetic architecture. In an ultimate approach to resolve this complexity, we intensified our phenotyping effort to target specifically a 3Mb-region known to segregate for a major quantitative trait gene, using a series of selected lines recombined every 100kb. We discovered that at least 3 other independent QTLs had remained hidden in this region, some with trait- or condition-specific effects, or opposite allelic effects. If we were to extrapolate the figures obtained on this specific region in this particular cross to the genome- and species-scale, we would predict hundreds of causative loci of detectable phenotypic effect controlling these growth-related phenotypes.
Changes in excitability and ion channel expression in neurons of the major pelvic ganglion in female type II diabetic mice
Authors: Gray Michael , Lett Kawasi M. , Garcia Virginia B. , Kyi Cindy W. , Pennington Kathleen A. , Schulz Laura C. , Schulz David J. 
Published: July 2018

ABSTRACTBladder cystopathy is a common urological complication of diabetes, and has been associated with changes in parasympathetic ganglionic transmission and some measures of neuronal excitability in male mice. To determine whether type II diabetes also impacts excitability of parasympathetic ganglionic neurons in females, we investigated neuronal excitability and firing properties, as well as underlying ion channel expression, in major pelvic ganglion (MPG) neurons in control, 10-week, and 21-week db/db mice. Type II diabetes in Leprdb/db animals caused a non-linear change in excitability and firing properties of MPG neurons. At 10 weeks, cells exhibited increased excitability as demonstrated by an increased likelihood of firing multiple spikes upon depolarization, decreased rebound spike latency, and overall narrower action potential half-widths as a result of increased depolarization and repolarization slopes. Conversely, at 21 weeks MPG neurons of db/db mice reversed these changes, with spiking patterns and action-potential properties largely returning to control levels. These changes are associated with numerous time-specific changes in calcium, sodium, and potassium channel subunit mRNA levels. However, Principal Components Analysis of channel expression patterns revealed that the rectification of excitability is not simply a return to control levels, but rather a distinct ion channel expression profile in 21-week db/db neurons. These data indicate that type II diabetes can impact the excitability of post-ganglionic, parasympathetic bladder-innervating neurons of female mice, and suggest that the non-linear progression of these properties with diabetes may be the result of compensatory changes in channel expression that act to rectify disrupted firing patterns of db/db MPG neurons.
Molecular basis for the acid initiated uncoating of human enterovirus D68
Authors: Liu Yue , Sheng Ju , Rossmann Michael G. 
Published: July 2018

AbstractEnterovirus D68 (EV-D68) belongs to a group of enteroviruses that contain a single positive-sense RNA genome surrounded by an icosahedral capsid. Like common cold viruses, EV-D68 mainly causes respiratory infections and is acid labile. The molecular mechanism by which the acid sensitive EV-D68 virions uncoat and deliver their genome into a host cell is unknown. Using cryo-electron microscopy (cryo-EM), we have determined the structures of the full native virion and an uncoating intermediate (the A(altered)-particle) of EV-D68 at 2.2 Å and 2.7 Å resolution. These structures showed that acid treatment of EV-D68 leads to particle expansion, externalization of the viral protein VP1 N-termini from the capsid interior, and formation of pores around the icosahedral two-fold axes through which the viral RNA can exit. Moreover, because of the low stability of EV-D68 at neutral pH, cryo-EM analyses of a mixed population of particles demonstrated the involvement of multiple structural intermediates during virus uncoating. Among these, a previously undescribed state, the expanded (“E1”) particle, shows a majority of internal regions (e.g, the VP1 N-termini) to be ordered as in the full native virion. Thus, the E1 particle acts as an intermediate in the transition from full native virions to A-particles. Molecular determinants, including a histidine-histidine pair near the two-fold axes, were identified that facilitate this transition under acidic conditions. Thus, the present work delineates the pathway of EV-D68 uncoating and provides the molecular basis for the acid lability of EV-D68 and of the related common cold viruses.Significance StatementEnterovirus D68 (EV-D68) is an emerging pathogen that primarily causes childhood respiratory infections and is linked to neurological diseases. It was unclear how the virus uncoats and delivers its genome into a host cell to establish viral replication. Using high resolution cryo-electron microscopy, we showed that acid induces structural rearrangements of EV-D68 to initiate genome release from the virus. Structural analyses delineated a viral uncoating pathway that involves multiple distinct conformational states. Particularly, the structure of a previously unknown uncoating intermediate enabled the identification of molecular determinants that facilitate EV-D68 uncoating in an acidic environment. These results advance the knowledge of cell entry of EV-D68 and open up possibilities for developing antiviral therapeutics that impede structural rearrangements of the virus.
The unexpected provenance of components in eukaryotic nucleotide-excision-repair and kinetoplast DNA-dynamics from bacterial mobile elements
Authors: Krishnan Arunkumar , Burroughs A. Maxwell , Iyer Lakshminarayan M. , Aravind L 
Published: July 2018

BackgroundProtein ‘weaponry’ deployed in biological conflicts between selfish elements and their hosts are increasingly recognized as being re-purposed for diverse molecular adaptations in the evolution of several uniquely eukaryotic systems. The anti-restriction protein ArdC, transmitted along with the DNA during invasion, is one such factor deployed by plasmids and conjugative transposons against their bacterial hosts.ResultsUsing sensitive computational methods we unify the N-terminal single-stranded DNA-binding domain of ArdC (ArdC-N) with the DNA-binding domains of the nucleotide excision repair (NER) XPC/Rad4 protein andTrypanosomaTc-38 (p38) protein implicated in kinetoplast(k) DNA replication and dynamics. We show that the ArdC-N domain was independently acquired twice by eukaryotes from bacterial mobile elements. One gave rise to the ‘beta-hairpin domains’ of XPC/Rad4 and the other to the Tc-38-like proteins in the stem kinetoplastid. Eukaryotic ArdC-N domains underwent tandem duplications to form an extensive DNA-binding interface. In XPC/Rad4, the ArdC-N domain combined with the inactive transglutaminase domain of a peptide-N-glycanase originally derived from an active archaeal version, often incorporated in systems countering invasive DNA. We also show that parallel acquisitions from conjugative elements and bacteriophages gave rise to the Topoisomerase IA, DNA polymerases IB-Ds, and DNA ligases involved in kDNA dynamics.ConclusionsWe resolve two outstanding questions in eukaryote-biology: 1) origin of the unique DNA lesion-recognition component of NER; 2) origin of the unusual, plasmid-like features of kDNA. These represent a more general trend in the origin of distinctive components of systems involved in DNA dynamics and their links to the ubiquitin system.