bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo evaluation of anticancer efficacy of drug loaded cockle shell‐derived aragonite nanoparticles
Authors: Wenliang Fu , Rameli Mohd Adha bin P. , Ibrahim Tengku Azmi Tengku , Noor Mohd Hezmee Mohd , Yusof Loqman Mohamad , Zakaria Md Zuki Abu Bakar 
Published: December 2018

AbstractDoxorubicin (DOX) is an effective and commonly used anthracycline anticancer drug for the treatment of osteosarcoma (OS). However, its antitumor effect is hampered by the nonspecific distribution and significant adverse effects. Nanoparticles based drug delivery systems are promising approaches to maximize the anticancer efficacy while decrease the side effects. In this study, biogenic aragonite nanoparticles (ANPs) were developed from cockle shells and loaded with DOX. An orthotopic rat OS model was induced by UMR‐106 cells tibia cavity injection. The anticancer efficacy study included five groups: normal control group, OS model group, free DOX group (2 mg/kg), DOX‐ANPs 1 group (2 mg of equivalent DOX/kg) and DOX‐ANPs 2 group (1.5 mg of equivalent DOX/kg). This study demonstrates that the DOX‐ANPs treatment groups can significantly reduce the tumor volume and increase the surviving ratio as compared to the OS model group. In addition, these two DOX‐ANPs groups showed less toxicity to the normal organs compared to the free DOX group. Furthermore, DOX‐ANPs 2 group showed the similar anticancer efficacy as DOX‐ANPs 1 group, which suggested that DOX loaded onto the ANPs may allow the reduction of chemotherapy doses. These results highlight the promising application of ANPs derived from cockle shells as an effective drug delivery system for a successful chemotherapy against OS. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1898–1907, 2019.
Butylene fipronil induces apoptosis in PC12 murine nervous cells via activation of p16‐CDK4/6‐cyclin D1 and mitochondrial apoptotic pathway
Authors: Zhong Shi , Zhu Jianxun , Li Yougui , Wang Xinquan , Yu Jiaqi , Ji Dongfeng , Wu Chongming 
Published: December 2018

AbstractButylene fipronil (BFPN) is a phenylpyrazole insecticide, acting at the γ‐aminobutyric acid (GABA) receptor. Here, we show that BFPN inducedcytotoxicity in PC12 murinenervous cells, which lacks GABA receptor. Treatment with BFPN for 48 hours significantly enhanced G0/G1 arrest and induced apoptosis. BFPN decreased the expression of cyclin‐dependent kinase (CDK4 and CDK6) and increased P16 and cyclin D1. Simultaneously, Bcl‐2 protein was declined while Bax and cytochrome c were significantly enhanced in BFPN‐treated groups. The apoptotic enzymes caspase‐8, ‐9, and ‐3 were also activated by BFPN. Furthermore, treatment with BFPN significantly stimulated reactive oxygen species (ROS) generation, and pretreatment with antioxidant diphenyleneiodonium, substantially reduced cell death. Overall, these results suggest that BFPN is effective to induce G0/G1‐phase arrest and apoptosis in PC12 murine nervous cell. Stimulating ROS generation and activation of P16‐CDK4/6‐cyclin D1 and mitochondrial apoptotic pathway may participate in the cytotoxicity of BFPN.
High doses of sodium tungstate can promote mitochondrial dysfunction and oxidative stress in isolated mitochondria
Authors: Cheraghi Ghazale , Hajiabedi Elnaz , Niaghi Behnaz , Nazari Firouzeh , Naserzadeh Parvaneh , Hosseini Mir‐Jamal 
Published: December 2018

AbstractTungstate (W) is recognized as an agent of environmental pollution and a substitute to depleted uranium. According to some preliminary studies, tungstate toxicity is related to the formation of reactive oxygen species (ROS) under abnormal pathological conditions. The kidneys and liver are the main tungstate accumulation sites and important targets of tungstate toxicity. Since the mitochondrion is the main ROS production site, we evaluated the mechanistic toxicity of tungstate in isolated mitochondria for the first time, following a two‐step ultracentrifugation method. Our findings demonstrated that tungstate‐induced mitochondrial dysfunction is related to the increased formation of ROS, lipid peroxidation, and potential membrane collapse, correlated with the amelioration of adenosine triphosphate and glutathione contents. The present study indicated that mitochondrial dysfunction was associated with disruptive effects on the mitochondrial respiratory chain and opening of mitochondrial permeability transition (MPT) pores, which is correlated with cytochrome c release. Our findings suggest that high concentrations of tungstate (2 mM)‐favored MPT pore opening in the inner membranes of liver and kidney mitochondria of rats. Besides, the results indicated higher tungstate susceptibility in the kidneys, compared with the liver.
A systematic review of the psychosocial impact of emotional numbing in US combat veterans
Authors: Schuman Donna L. , Bricout John , Peterson H. Leona , Barnhart Sheila 
Published: December 2018

AbstractContextPrevious studies have linked posttraumatic emotional numbing symptoms in US combat veterans with an adverse impact in multiple important life domains.ObjectivesWe updated and evaluated the evidence examining the psychosocial impact of combat‐related emotional numbing, including ethnoracial and gender differences.MethodWe reviewed 1,209 articles published betwen January 2012 and 2018 and selected 24 studies for inclusion. We assessed the overall study quality as fair using a national quality assessment tool.ResultsStudies found emotional numbing to wield adverse effects in the areas of symptom nonimprovement, mental health difficulties, increased service utilization, poor relationship functioning, reduced quality of life, substance use disorders, suicidality, and aggression/violence. We also found evidence of ethnoracial and gender differences in veterans’ posttraumatic stress disorder‐related emotional numbing symptoms.ConclusionClinicians should incorporate findings on emotional numbing into assessment, treatment planning, and monitoring, to improve treatment retention and psychosocial outcomes. Implications for ethnoracial and gender differences require further exploration.
The role of therapist and patient in‐session behavior for treatment outcome in exposure‐based cognitive behavioral therapy for panic disorder with agoraphobia
Authors: Maiwald Lisa M. , Junga Yvonne M. , Lang Thomas , Montini Romina , Witthöft Michael , Heider Jens , Schröder Annette , Weck Florian 
Published: December 2018

AbstractObjectiveThere is a very limited amount of research on the relationship between therapist and patient in‐session behavior and treatment outcome in cognitive behavioral therapy (CBT) for panic disorder with agoraphobia (PD/AG). Additionally, the findings tend to be inconclusive. This study investigates the association between therapist competence, adherence, patient interpersonal behavior, and therapeutic alliance and outcome in a low‐control CBT setting by using comprehensive measures.MethodsTwenty‐six patients with PD/AG received 12 sessions of exposure‐based CBT. With regard to the outcome, treatments were classified either as problematic or nonproblematic by means of distinct criteria. Two raters evaluated the in‐session behavior.ResultsPatient interpersonal behavior was significantly associated with outcome at follow‐up (r = 0.49). At posttreatment, the correlation did not reach significance (
r = 0.34). Competence, adherence, and alliance were not outcome associated.ConclusionThe findings emphasize the need for therapists to pay particular attention to patients’ interpersonal behavior during treatment.
Pretreatment counseling experiences, stressors, and support differences between transgender and cisgender university students seeking mental healthcare
Authors: Bartholomew Theodore T. , Gundel Brittany E. , Sullivan John W. , Pérez‐Rojas Andrés E. , Lockard Allison J. 
Published: December 2018

AbstractObjectivesOur aim was to explore rates of prior counseling experiences as well as pretreatment stressors and supports among transgender university students seeking psychotherapy services in university counseling centers.MethodsWe used regression models to explore relationships between gender identity and prior mental health experiences, risk‐related experiences, traumatic experiences, and support among clients (cisgender: n = 162,305; transgender: n = 545) seeking treatment at 136 university counseling centers.ResultsResults demonstrate more previous mental health service utilization and greater frequency of some prior stressors transgender clients. Findings indicate the odds of transgender clients reporting drug and alcohol concerns are lower than the reference group. Additionally, transgender clients report less familial support but more social support than cisgender clients.ConclusionWe discuss the importance of these findings through a more holistic lens that recognizes both stressors as well as positive factors that may more accurately characterize the experiences of transgender clients.
Engagement in linkage to mental health care program in the Rockaways after Hurricane Sandy
Authors: Bevilacqua Kristin , Schneider Samantha , Rasul Rehana , Taioli Emanuela , Schwartz Rebecca M. 
Published: December 2018

AbstractThe Rockaways area of New York City was especially devastated by Hurricane Sandy. This study examined participant characteristics associated with acceptance of a linkage to and attendance at appointments for mental health difficulties (MHD). Participants (N = 1,011) completed questionnaires to assess mental health symptoms. Participants who met screening criteria (n = 442) were offered linkage to care. Individuals who had a higher mental health symptom burden (MHSB; i.e., those who screened in on more than one criteria vs. only one criterion) had 2.68 greater odds of accepting services (95% confidence interval [1.68, 4.26]). MHSB was not associated with attending a first appointment (p = 0.80). Female gender and Hispanic ethnicity were also associated with acceptance of linkage to care, though not attendance. Reducing stigma around MHD associated with natural disasters and increasing knowledge about the mental health care system could promote help‐seeking behavior among survivors.
Antimicrobial activity of intracanal medications against both Enterococcus faecalis and Candida albicans biofilm
Authors: Zancan Rafaela Fernandes , Calefi Pedro Henrique Souza , Borges Mariana Maciel Batista , Lopes Marcelo Ribeiro Milanda , de Andrade Flaviana Bombarda , Vivan Rodrigo Ricci , Duarte Marco Antonio Hungaro 
Published: December 2018

AbstractEnterococcus faecalis and Candida albicans have been associated with cases of secondary and persistent root canal infections, been resistant to calcium hydroxide. So, the evaluation of the susceptibility of these microorganisms biofilms to new drugs is an important practice for establishing the best drug and consequently success of treatment. For this, in vitro biofilm formation of E. faecalis and C. albicans was induced separately on blocks obtained from bovine teeth. After the period of specimen incubation for biofilm maturation, the samples were immersed in the pastes: 1 – calcium hydroxide (CH), 2 – chlorhexidine (C), 3 – ciprofloxacin (CP), 4 – metronidazole (MT), 5 – ketoconazole (KE), 6 – double antibiotic (DB), 7 – triple antibiotic (TA), 8 – ciprofloxacin + ketoconazole (CPKE); 9 – ciprofloxacin + metronidazole + ketoconazole (CPMTKE), 10 – metronidazole + ketoconazole (MTKE), and 11 – control (CO) for 7 days. Next, the specimens were live/dead stained for analysis by confocal microscopy. By means of the Bioimage program, the biovolume and percentage of live cells were measured. The data were statistically compared (p = .05). For the C. albicans biofilm, the best antimicrobial action was found for MTKE, CPKE, and MT groups. Whereas for E. faecalis biofilm, the lowest percentage of live bacteria was found in TA, DB, and CP groups; however, KE, CPKE, CPMTKE, and MTKE groups shown to be effective. The authors concluded calcium hydroxide paste and chlorhexidine was not effective for both biofilms. The MTKE and CPKE pastes presented effectiveness for both biofilms. TA and DB pastes were effective just in the E. faecalis biofilms.
Patient goals, motivations, and attitudes in a patient‐driven variant reclassification study
Authors: Tsai Ginger J. , Garrett Lauren Thomas , Makhnoon Sukh , Bowen Deborah J. , Burke Wylie , Shirts Brian H. 
Published: December 2018

AbstractFamily studies to reclassify clinically ascertained variants of uncertain significance (VUS) can impact risk assessment, medical management, and psychological outcomes for patients and their families. There are limited avenues for patients and their families to actively participate in VUS reclassification, and access to family studies at most commercial laboratories is restricted by multiple factors. To explore patient attitudes about participation in family studies for VUS reclassification, we conducted semistructured pre‐ and post‐participation telephone interviews with 38 participants in a family‐based VUS reclassification study that utilized a patient‐driven approach for family ascertainment and recruitment. Participants had VUS from multigene panel testing performed at multiple clinical laboratories for cancer or other disease risk. Inductive thematic analysis of transcribed interviews highlighted four major themes: (a) Participants’ study goals were driven by the desire to resolve uncertainty related to the VUS, (b) Participants had mixed reactions to the VUS reclassification outcomes of the study, (c) Personal, public, and familial knowledge increased through study participation and (d) Participants used study participation to actively cope with the uncertainty of a VUS. As personalized genomic medicine becomes more prevalent, clinicians, clinical laboratories, and researchers could consider creating more opportunities for active partnership with patients and families, who are motivated to contribute data to familial VUS studies.
Single MRI‐Based Volumetric Assessment in Clinical Practice Is Associated With MS‐Related Disability
Authors: D'hooghe Marie B. , Gielen Jeroen , Van Remoortel Ann , D'haeseleer Miguel , Peeters Erika , Cambron Melissa , De Keyser Jacques , Nagels Guy 
Published: December 2018

BackgroundThe added value of brain volume measurements in the clinical practice of multiple sclerosis (MS) has been questioned.PurposeTo investigate the contribution of volume measures obtained with magnetic resonance scans performed as part of regular care to predict measures of cognitive and physical MS disability in a real‐world setting.Study TypeRetrospective.SubjectsIn all, 470 adults with diagnosed MS.Field Strength/Sequence3D fluid attenuation inversion recovery (FLAIR) and 3D T1‐weighted MR images at 3.0T MR.AssessmentLesion and brain volume were measured by an automated method, MSmetrix, developed by icometrix.Statistical TestsWe used stepwise linear regression models to assess the added value of a single volumetric assessment in predicting Expanded Disability Status Scale (EDSS) and Symbol Digit Modalities Test (SDMT). Brain volumes categorized into quartiles were used as predictive variables in a time‐to‐event analysis and Cox proportional hazard regression with time to worsening from baseline as outcome measures.ResultsBrain and lesion volume in relapsing onset MS strongly contributed to the best models, with a substantial role for age in the EDSS model and a modest role for education in the SDMT model. Adding MR volumetric information increased the explained variance from 17% to 28% in the best model for EDSS and from 9% to 25% in the best model for SDMT. A significantly reduced hazard (P < 0.05) of SDMT worsening was found in the highest normalized brain volume quartiles (1375–1608 ml), compared with the lowest quartile (1201–1374 ml) in the total study population.Data ConclusionOur findings indicate that a single brain volumetric assessment contributes to the prediction of MS‐related disability, with distinct patterns for EDSS as a measure of physical disability, and SDMT as a measure of cognitive disability. A threshold effect for the lowest brain volumes with regard to SDMT worsening over time was found.Level of Evidence: 3Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;49:1312–1321.
Challenges in the identification process of phenidate analogues in LC‐ESI‐MS/MS analysis: Information enhancement by derivatization with isobutyl chloroformate
Authors: Shamai Yamin Tamar , Prihed Hagit , Weissberg Avi 
Published: December 2018

AbstractA new analytical technique for the structural elucidation of four representative phenidate analogues possessing a secondary amine residue, which leads to a major/single amine‐representative fragment/product ion at m/z 84 both in their GC‐EI‐MS and LC‐ESI‐MS/MS spectra, making their identification ambiguous, was developed. The method is based on “in vial” chemical derivatization with isobutyl chloroformate in both aqueous and organic solutions, followed by liquid chromatography‐electrospray ionization mass spectrometry (LC‐ESI‐MS/MS). The resulting carbamate derivatives promote rich fragmentation patterns with full coverage of all substructures of the molecule, enabling detailed structural elucidation and unambiguous identification of the original compounds at low ng/mL levels.
Tra2β silencing suppresses cell proliferation in laryngeal squamous cell carcinoma via inhibiting PI3K/AKT signaling
Authors: Ni Hao‐Sheng , Hu Song‐Qun , Chen Xi , Liu Yi‐Fei , Ni Ting‐Ting , Cheng Lei 
Published: December 2018

Objectives/HypothesisTransformer‐2 protein homolog beta (Tra2β) generally plays an important role in various human cancers, but its role and the underlying mechanisms in laryngeal squamous cell carcinoma (LSCC) remained unknown. So this study aimed to assess the clinical significance and regulatory mechanisms of Tra2β in LSCC.Study DesignLaboratory analysis.MethodsExpression of Tra2β was compared in human LSCC tissue samples and paired adjacent normal tissue samples. The in vitro effects of Tra2β expression in Hep‐2 cells on their proliferation, invasion, and migration were assessed by CCK‐8 assays, Matrigel invasion, and transwell migration assays. In addition, the effects of downregulation of Tra2β on the activation of PI3K/AKT signaling pathway were measured using Western blot analysis. The effect of Tra2β on the growth of tumors was detected in the Hep‐2–injected xenograft models in vivo.ResultsReverse‐transcription quantitative polymerase chain reaction analysis and immunochemistry analysis indicated that the increased expression of Tra2β in LSCC was significantly associated with poor differentiation, lymph node metastasis, and advanced clinical stage. In vitro knockdown of Tra2β caused a significant decrease in the proliferation, invasion, and migration of Hep‐2 cells. Tra2β silencing decreased the expression of Bcl‐2 but increased Bax and Caspase‐3 both in mRNA and protein levels. Furthermore, knockdown of Tra2β eliminated the suppressive effects of activation of PI3K/AKT signaling. In vivo knockdown of Tra2β significantly inhibited the tumor growth of Hep‐2–injected xenograft mice.ConclusionsThe results of the present study demonstrated that knockdown of Tra2β inhibits the proliferation and invasion of LSCC cells, at least partly via inhibiting PI3K/AKT signaling.Level of EvidenceNALaryngoscope, 129:E318–E328, 2019
In‐band phase deviation minimization method for wideband tunable phase shifter
Authors: Chaudhary Girdhari , An Boram , Jeong Yongchae 
Published: December 2018

AbstractThis article provides a novel approach to analyze and design wideband tunable reflection‐type phase shifter (RTPS) with low in‐band phase deviation (IBPD) error. The proposed RTPS consists of coupled lines where through and coupled ports are terminated with varactor diodes. The coupled lines can minimize the parasitic elements of varactor diode to achieve high phase shift range (PSR). In addition, the measured frequency‐dependent capacitance of varactor diode was used in the analytical analysis to obtain minimum IBPD error and high PSR. For experimental demonstration, phase shifter was fabricated at the center frequency of 2.5 GHz. The measured results show that the fabricated circuit achieved 126.85° PSR with ±6.48° IBPD error for 500 MHz bandwidth (BW). In addition, the measured maximum insertion loss variation and input/output return losses are within ±0.34 dB and higher than 16.85 dB within 500 MHz BW, respectively.
Co‐polarized monostatic antenna with high Tx–Rx isolation for 2.4 GHz single channel full duplex applications
Authors: Nawaz Haq , Gürbüz Özgür , Tekin Ibrahim 
Published: December 2018

AbstractThis article presents a linear co‐polarized (same polarization for transmit and receive modes), proximity coupled monostatic patch antenna with high port to port RF isolation for 2.4 GHz single channel full duplex (SCFD) wireless applications. The presented proximity coupled monostatic patch antenna deploys differential feeding for receive (Rx) mode to significantly suppress RF leakage from transmit (Tx) port and external single‐tap self‐interference cancellation (SIC) circuit provides additional interport isolation. The measurements for implemented prototype of antenna in lab environment provide around 50 dB Tx–Rx isolation for 50 MHz bandwidth in addition to better than 75 dB peak isolation. Moreover, the deployed single‐tap RF SIC circuit can be used to suppress SI resulted from environmental reflections and 50 MHz SIC bandwidth with 50 dB isolation can be tuned within antenna's 10 dB return loss impedance bandwidth of 150 MHz.
The relationship between teachers’ emotional intelligence and classroom discipline management
Authors: Valente Sabina , Monteiro Ana Paula , Lourenço Abílio Afonso 
Published: December 2018

AbstractOne of the fundamental problems of educational systems in many countries is related to classroom discipline. This reflects one of the worst problems faced by teachers. Classroom discipline management strategies play an effective role in creating positive teacher‐student relationships. One of the factors that influence behavior management in a classroom is emotional intelligence. Therefore, this study analyzing how teachers emotional intelligence influences the management of discipline in a classroom and the relationship between gender, academic formation, and service time of teachers with their emotional intelligence. Its sample comprises 559 basic and secondary school teachers. An Emotional Competence Questionnaire, a Scale of Teacher Efficacy in Classroom Management, and a personal and professional data inquiry have been used as instruments. Results show that teachers who have more capacity to deal with emotion demonstrate a greater management of discipline in the classroom. Most of the relationships in the model are statistically significant.
Parent question use during shared reading time: How does training affect question type and frequency?
Authors: Zibulsky Jamie , Casbar Christine , Blanchard Tamar , Morgan Chelsea 
Published: December 2018

AbstractThis study was designed to examine whether participation in a shared reading workshop alters the frequency with which parents ask their children questions during book reading sessions, particularly questions designed to strengthen component reading skills that they may not have known about before training. Participants in the reading workshop series (N = 57) were taught strategies for asking questions about story content and word structure to build children's language and literacy skills. Findings suggest that parents may be somewhat familiar with traditional dialogic reading strategies focused on story content and utilize them without instruction, whereas parents may be less knowledgeable about sound or print‐focused skills and do not employ strategies focused on word structure until instructed to do so. It is also notable that parents do not use all story content prompts equally. This information can be used by school psychologists to refine the messages educators share with parents about how to best support their children's reading development.