bluuebunny/crossref_metadata_2025_split huggingface dataset:
Subcellular distributions of iron oxide nanoparticles in rat brains affected by different surface modifications
Authors: Wang Sheng , Zhang Baolin , Su Lichao , Nie Wan , Han Dong , Han Guihua , Zhang Hao , Chong Chuangang , Tan Jie 
Published: May 2019

AbstractThe impact of the surface modification on the subcellular distribution of nanoparticles in the brain remains elusive. The nanoparticles prepared by conjugating polyethylene glycol and maleic anhydride‐coated superparamagnetic iron oxide nanoparticles (Mal‐SPIONs) with bovine serum albumin (BSA/Mal‐SPIONs) and with Arg–Gly–Asp peptide (RGD/Mal‐SPIONs) were injected into the rat substantia nigra. Observation of transmission electron microscopy (TEM) samples obtained 24 h after perfusion showed that abundant RGD/Mal‐SPIONs accumulated in the myelin sheath, dendrites, axon terminals and mitochondria, and on cell membranes in the brain tissue near the injection site. For rats injected with BSA/Mal‐SPIONs, a few nanoparticles accumulated in the myelin sheath, axon terminals, endoplasmic reticulum, mitochondria, Golgi, and lysosomes of neurons and glial cells while least SPIONs in rats injected with Mal‐SPIONs were found. TEM pictures showed some Mal‐SPIONs were expelled out of the brain. RGD/Mal‐SPIONs diffused extensively to the thalamus, frontal cortex, temporal lobe, olfactory bulb, and brain stem after injection. Only a few BSA/Mal‐SPIONs diffused to the afore‐mentioned brain areas. This work reveals different surface modifications on the iron oxide nanoparticles play crucial roles in their distribution and diffusion in the rat brains.
3‐(3‐Hydroxyphenyl)‐Propionic Acid (PPA) Suppresses Osteoblastic Cell Senescence to Promote Bone Accretion in Mice
Authors: Chen Jin‐Ran , Wankhade Umesh D , Alund Alexander W , Blackburn Michael L , Shankar Kartik , Lazarenko Oxana P 
Published: May 2019

ABSTRACTPhenolic acids (PAs) are metabolites derived from polyphenolic compounds found in fruits and vegetables resulting from the actions of gut bacteria. Previously, we reported that the levels of seven individual PAs were found to be at least 10 times higher in the serum of rats fed a blueberry (BB)‐containing diet compared to those fed a control diet. We have characterized the effects of one such BB‐associated serum PA, 3‐(3‐hydroxyphenyl)‐propionic acid (PPA), on senescence signaling and promotion of mesenchymal stem cell differentiation toward osteoblasts, while suppressing adipogenesis in the stem cells. To better understand the mechanistic actions of PPA on bone formation in vivo, we administered four doses of PPA (0.1, 0.5, 1, and 5 mg/kg/day; daily i.p.) to 1‐month‐old female C57BL6/J mice for 30 days. We did not observe significant effects of PPA on cortical bone; however, there were significantly higher bone volume and trabecular thickness and increased osteoblastic cell number, but decreased osteoclastic cell number in PPA‐treated groups compared to controls. These morphological and cellular outcomes of bone were reflected in changes of bone formation markers in serum and bone marrow plasma. PPA treatment reduced senescence signaling as evaluated by senescence‐associated β‐galactosidase activity, PPARγ, p53, and p21 expression in bone. In conclusion, PPA is capable of altering the mesenchymal stem cell differentiation program and bone cell senescence. This raises the possibility that BB‐rich diets promote bone growth through increasing systemic PAs, a question that merits additional investigation. © 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.
Inhibition of myostatin improves muscle atrophy in oculopharyngeal muscular dystrophy (OPMD)
Authors: Harish Pradeep , Malerba Alberto , Lu‐Nguyen Ngoc , Forrest Leysa , Cappellari Ornella , Roth Fanny , Trollet Capucine , Popplewell Linda , Dickson George 
Published: May 2019

AbstractBackgroundOculopharyngeal muscular dystrophy (OPMD) is a late‐onset muscle disease affecting one per 80 000 of the general population characterized by profound dysphagia and ptosis, and limb weakness at later stages. Affected muscles are characterized by increased fibrosis and atrophy. Myostatin is a negative regulator of muscle mass, and inhibition of myostatin has been demonstrated to ameliorate symptoms in dystrophic muscles.MethodsIn this study, we performed a systemic delivery of a monoclonal antibody to immunologically block myostatin in the A17 mouse model of OPMD. The mice were administered a weekly dose of 10 mg/kg RK35 intraperitonially for 10 weeks, following which histological analyses were performed on the samples.ResultsThis treatment significantly (P< 0.01) improved body mass (11%) and muscle mass (for the tibialis anterior and extensor digitorum longus by 19% and 41%) in the A17 mice treated with RK35 when compared to saline controls. Similarly, a significantly (P< 0.01) increased muscle strength (18% increase in maximal tetanic force) and myofibre diameter (17% and 44% for the tibialis anterior and extensor digitorum longus), and reduced expression of markers of muscle fibrosis (40% reduction in area of expression), was also observed. No change in the density of intranuclear inclusions (a hallmark of disease progression of OPMD) was however observed.ConclusionsOur study supports the clinical translation of such antibody‐mediated inhibition of myostatin as a treatment of OPMD. This strategy has implications to be used as adjuvant therapies with gene therapy based approaches, or to stabilize the muscle prior to myoblast transplantation.
Implementing Quality Assessment Is Fundamental to Guarantee Optimal Nutrition Therapy
Authors: Gomes Mel Mariá Assunção , da Silva Jessica Moreira , Silva Ferreira Álida Rosária , de Vasconcelos Generoso Simone , Correia Maria Isabel Toulson Davisson 
Published: May 2019

AbstractIntroductionThe implementation of quality assessment in nutrition therapy (NT) is an important strategy, with the potential to contribute to the reduction of malnutrition and improve the cost‐effectiveness of NT. Studies on this subject are scarce in the world. Our objective was to evaluate the practice of NT in hospitals located in the third‐most‐developed state of Brazil.MethodsA cross‐sectional study, using data collected through interviews with members of the clinical staff/interdisciplinary NT team (NTT) and audits of clinical protocols and medical records, was carried out. Both non–probability and probability sampling techniques were used to select the hospitals.ResultsThirty‐three hospitals were included (10 public, 10 philanthropic, 9 private, and 4 university). Twenty‐six institutions reported to have an NTT. There was no statistically significant difference in the existence and performance of the teams or in the presence of protocols and quality indicators between the types of hospitals. However, the inspection of 172 medical records showed that essential items for the nutrition care of the patient were not fully in agreement with the previous provided information, and this varied according to the type of hospital.ConclusionThe majority of hospitals had an NTT as well as protocols and quality indicators. However, patient records were not in accordance with the essential items to guarantee NT quality and safety, indicating a gap between theory and practice.
Patient‐reported auditory handicap measures following mild traumatic brain injury
Authors: Knoll Renata M. , Herman Seth D. , Lubner Rory J. , Babu Ashwin N. , Wong Kevin , Sethi Rosh K. V. , Chen Jenny X. , Rauch Steven D. , Remenschneider Aaron K. , Jung David H. , Kozin Elliott D. 
Published: May 2019

Objectives/HypothesisFew studies have specifically addressed auditory complaints in patients with nonblast mild traumatic brain injury (mTBI). Herein, we aimed to investigate auditory symptoms in patients following mTBI using patient‐reported outcome measures.Study DesignRetrospective analysis of prospectively collected data in a tertiary‐care hospital.MethodsThe patients included those with mTBI (cases) and those without mTBI (controls). Individuals (≥18 years old) with and without mTBI were screened. Exclusion criteria included history of otologic disorders, blast injury, or occupational noise exposure. Primary outcomes included the Hearing Handicap Inventory for Adults (HHIA), Tinnitus Handicap Inventory (THI), and Hyperacusis Questionnaire (HQ). Secondary outcomes included subjective auditory complaints.ResultsFrom September 2017 to September 2018, 52 patients with mTBI and 55 controls met inclusion and exclusion criteria. The mean time between mTBI and survey intake was 70.6 months. The mean age and gender were 51.5 years old and 73% female in the mTBI group, and 46.1 years old and 56.3% female in the control group (P = .112 and P = .105, respectively). Patients with mTBI reported hyperacusis (67.3% of all mTBI patients), hearing loss (61.5%), and tinnitus (61.5%), compared to 8.3%, 12.7%, and 16.4%, respectively, for control subjects (P < .0001). The mean HHIA score in the mTBI group was 38.3 versus 8.5 in controls (P = .002). The mean THI score was 27.4 in the mTBI group and 3.1 in controls (P < .0001). The mean HQ score was 26.5 in mTBI group and 7.3 in controls (P = .001).ConclusionsAuditory symptoms and associated handicap were common in patients with nonblast mTBI compared to age‐matched controls. Findings have implications for the pathophysiology and management of symptoms in this patient population.Level of Evidence3Laryngoscope, 130:761–767, 2020
A New Strategy to Synthesize α,ω‐Dihydroxy Multiblock Copolymers via [CpRu(CH3CN)3]PF6/Quinaldic Acid Catalyst
Authors: Zhu Xiuzhong , Wang Zichao , Liu Jie , Min Xin , Wang Tong , Fan Xiaodong 
Published: May 2019

AbstractIn this study, a new strategy to synthesize random and alternating multiblock copolymers (MBCs) by the polycondensation of macromonomers’ terminal hydroxyl groups with [CpRu(CH3CN)3]PF6/quinaldic acid as the catalyst is reported, which is often used for the preparation of a variety of biological small molecules via the reaction of allyl ethers. The degrees of hydroxyl functionality (Fn) of the MBCs are assessed by titration, and the presence of hydroxyl on both the ends of MBCs is also confirmed by a chain‐extension experiment of the ring‐opening polymerization of D,L‐lactide.
Maternal High‐Sucrose Diet Accelerates Vascular Stiffness in Aged Offspring via Suppressing Cav1.2 and Contractile Phenotype of Vascular Smooth Muscle Cells
Authors: Feng Xueqin , Li Xiang , Yang Chunli , Ren Qinggui , Zhang Wenna , Li Na , Zhang Meng , Zhang Bo , Zhang Lubo , Zhou Xiuwen , Xu Zhice 
Published: May 2019

ScopeThe fetal programming in response to over‐nutrition during pregnancy is involved in pathogenesis of cardiovascular diseases later in life. The authors’ previous work reported that prenatal high‐sucrose (HS) diet impaired functions of large‐conductance Ca2+‐activated K+ channels (BK) in mesenteric arteries in the adolescent offspring rats. This study determines whether prenatal HS has a long‐term impact on resistance vasculature in the aged offspring rats.Methods and resultsPregnant rats are fed with a high‐sucrose diet until delivery. Aged offspring from prenatal HS exhibit elevated fasting insulin level, insulin resistance index, and diastolic pressure. Both pressure‐induced myogenic responses and phenylephrine‐stimulated contraction of mesenteric arteries in HS are weakened. Electrophysiological tests and western blot indicate that BK and L‐type calcium channels (Cav1.2) are impaired in HS group. On the other hand, expression of matrix metalloproteinase 2 of mesenteric arteries is reduced in HS group while expression of tissue inhibitors of metalloproteinase is increased, indicating that extra cellular matrix (ECM) is remodeled. Furthermore, expression of α‐smooth muscle actin is decreased, and insulin/insulin receptor/phosphoinositide3‐kinase (PI3K) signaling pathway is downregulated.ConclusionThe results suggest that prenatal HS induced stiffness of mesenteric arteries in aged offspring by inhibiting Cav1.2 function and PI3K‐associated contractile phenotype of VSMCs.
Metamaterial‐inspired low‐profile high‐gain slot antenna
Authors: Pandit Soumen , Mohan Akhilesh , Ray Priyadip 
Published: May 2019

AbstractThis article presents a novel polarization‐independent metamaterial (MTM) which is used for the design of a low‐profile high‐gain magnetic dipole antenna operating at 9.8 GHz. The MTM is alike star‐shape and shows low impedance property around the frequency 9.8 GHz. The reference antenna, which is the SIW‐based slot antenna acts as a magnetic dipole antenna and it has peak gain of 3 dBi. The metamaterial‐inspired slot antenna has low‐profile, that is, 0.133 λ0 at the frequency 9.8 GHz, and peak gain of 8.24 dBi. At the resonance frequency, more than 5 dB gain improvement is achieved in the proposed design. These results are verified with the fabrication of a prototype antenna and measurements. The cross‐polarized radiation is negligibly altered by this gain enhancement method. The proposed antenna can be used in wireless power transfer and LoS communications.
Characterizing Angler Preferences for Largemouth Bass, Bluegill, and Walleye Fisheries in Wisconsin
Authors: Tingley Ralph W. , Hansen Jonathan F. , Isermann Daniel A. , Fulton David C. , Musch Andrea , Paukert Craig P. 
Published: May 2019

Abstract
Managing recreational fisheries in lake-rich landscapes with diverse fish communities and anglers alike presents a social and biological challenge for managers. Understanding angler preferences is central to navigating these challenges and can aid in predicting shifts in angler behavior in response to management actions or changing fish populations. Species-specific angler surveys do not incorporate tradeoffs inherent in multispecies fisheries, thus limiting their application to real-world management issues. To better understand angler preferences in relation to realistic tradeoffs among different fishing opportunities, we conducted a survey of Wisconsin anglers in 2013–2014 that included questions requiring anglers to choose between fisheries providing different outcomes. We used stated-preference modeling to quantify angler preferences associated with Bluegill Lepomis macrochirus, Largemouth Bass Micropterus salmoides, and Walleye Sander vitreus fisheries. Next, we conducted a latent class analysis using survey responses to classify anglers into subgroups. Finally, we assessed the sensitivity of angler choice to changes in fishing opportunities. Results of the stated-preference models indicated that both residents and nonresidents largely prefer “quality” (i.e., moderate catch rate and size structure) over “action” or “trophy” Bluegill fisheries and that characteristics of Largemouth Bass fisheries have more value to nonresidents than to residents. Aspects of the Bluegill fishery were most important in choosing among hypothetical lakes for resident respondents. Five angler subgroups were identified that show the importance of Bluegill and Walleye but shed additional light on anglers’ commitment level and nonresidents’ preference for Largemouth Bass. In addition, simulations of changes in Walleye, Largemouth Bass, and Bluegill fisheries indicated that maintenance of quality Bluegill fisheries is important to ensuring continued angler participation, while the retention of high-yield Walleye fisheries is likely paramount to a subgroup of anglers. Our results offer insight into angler preferences across the lake-rich landscape of Wisconsin and demonstrate how angler behavior may shift in response to transitions in fishing opportunities.
Sleep Duration and Adiposity in Children and Adults: Observational and Mendelian Randomization Studies
Authors: Wang Jiao , Li Albert M. , Lam Hugh S. Hung San , Leung Gabriel M. , Schooling C. Mary 
Published: May 2019

ObjectiveThis study used two complementary designs, an observational and a Mendelian randomization (MR) study, to assess whether sleep duration causes adiposity in children and adults.MethodsIn Hong Kong's “Children of 1997” birth cohort, the adjusted cross‐sectional associations of sleep duration with BMI z score and obesity and overweight were assessed at ~11 years of age. Generalized estimating equations were also used to examine longitudinal associations of sleep duration at ~11 years with annual BMI z score and obesity and overweight at about 11 to 16 years of age. Using MR, this study assessed the association of genetically predicted sleep duration, based on 54 single‐nucleotide polymorphisms, applied to genetic studies of adiposity in children (n = 35,668), men (n = 152,893), and women (n = 171,977).ResultsLonger sleep was cross‐sectionally associated with lower BMI z score at ~11 years of age (−0.13 per category, 95% CI: −0.22 to −0.04) and at about 11 to 16 years of age longitudinally in girls (−0.39, 95% CI: −0.66 to −0.13). Using MR, sleep duration was inversely associated with BMI in children (−0.29 SD per hour, 95% CI: −0.54 to −0.04), but was not clearly associated with BMI in adults, particularly for women.ConclusionsA small beneficial effect of sleep on BMI in children cannot be ruled out.
Identification of critical success factors for public–private partnership projects
Authors: Sehgal Reetika , Dubey Ashish Mohan 
Published: May 2019

Public–private partnership (PPP) is one of the popular mechanisms for the development of infrastructure worldwide; governments across the globe are engaged in discovering a range of PPP policies and strategies for growth of infrastructure.The growth of infrastructure is imperative of the sustainable development of an economy. Therefore, it is pertinent to identify key success factors for the development of infrastructure through PPP model. The current paper aims to explore significant factors for successful execution and completion of PPP projects in infrastructure. A total of 517 employees who had hand‐on experience on PPP projects located in India were interviewed through a structured questionnaire. Exploratory factor analysis yielded 14 components as a significant factor for the success of PPP in infrastructure. This study highlights that the development of infrastructure would be rapid through PPP if government considers these factors in the implementation phase.
AYA testis cancer: The unmet challenge
Authors: Stoneham Sara , Murray Matthew , Thomas Benjamin , Williamson Max , Sweeney Christopher , Frazier Lindsay 
Published: May 2019

AbstractTestis cancer is considered a rare‐incidence cancer but comprises the third most common cancer diagnosed within the adolescent and young adult (AYA) years (15–39 years). Most testis cancer patients can anticipate a survival outcome in excess of 95%. However, there are subgroups of AYA patients where outcomes are considerably worse, including younger adolescents, patients with certain histological subtypes, or from certain ethnic backgrounds. For those cured with chemotherapy, the toxicity of treatment and burden of late effects is significant. Newer germ cell tumor–specific biomarkers may identify patients who do not require further treatment interventions or may detect early recurrence, potentially reducing the burden of treatment required for cure. An international collaboration for this rare tumor is creating the forum for trial design, where these biomarker research questions are embedded. Going forward, AYA testis cancer patients could benefit from having a more personalized treatment plan, tailored to risk, that minimizes the overall burden of late effects.
Interference of bone marrow CD56+ mesenchymal stromal cells in minimal residual disease investigation of neuroblastoma and other CD45−/CD56+ pediatric malignancies using flow cytometry
Authors: Theodorakos Ioannis , Paterakis Georgios , Papadakis Vassilios , Vicha Ales , Topakas Georgios , Jencova Pavla , Karchilaki Eirini , Taparkou Anna , Tsagarakis Nikolaos J. , Polychronopoulou Sophia 
Published: May 2019

AbstractBackgroundBone marrow (BM) samples obtained from minimal residual disease (MRD)‐negative children with B‐cell acute lymphoblastic leukemia (B‐ALL) were used in our laboratory as negative biological controls for the development of a neuroblastoma (NBL) flow‐cytometric (FC) protocol. The accidental, but systematic, identification of rare cell populations (RCP) mimicking NBL cells (CD45−/CD56+) in these samples indicated the need for their thorough immunophenotypic identification, in order to elucidate their possible interference in NBL‐MRD assessment.ProcedureRCP observed in BM samples from 14 children recovering from BM aplasia due to intensive chemotherapy for B‐ALL were investigated with the following markers: CD81, CD200, CD24, GD2, CD73, CD13, CD90, CD146, CD9, CD117, CD10, CD99, and NG2. BM samples from six newly diagnosed patients with NBL and an NBL cell line were simultaneously investigated as positive controls.ResultsThe frequency of RCP in B‐ALL BM samples was < 1/1 × 104 cells (bulky lysis), and their immunophenotypic profile was indicative of CD56+ mesenchymal stromal cells (MSCs) (CD45−, CD90+, CD146+, CD73+). Also, RCP expressed CD81 and CD200, simulating NBL cells. The most useful discriminative markers for CD56+ MSCs were CD13 and CD73. An appropriate protocol consisting of two tubes with seven color combinations was further proposed: SYTO‐16, GD2 (first tube) or CD73 (second tube)‐PE, CD24‐ECD, CD13‐PC5.5, CD45‐PC7, CD81‐APC, and CD56‐APC700.ConclusionsRCP that were immunophenotypically similar to NBL were identified as CD56+ MSCs. As these cells might pose an obstacle to accurate NBL disease assessment by FC, especially MRD, an enhanced NBL‐FC protocol is proposed for prospective evaluation.
Peer attachment and grit in adolescence and emerging adulthood
Authors: Lan Xiaoyu 
Published: May 2019

AbstractThis study examined the moderating role of developmental stage (adolescence vs. emerging adulthood) in the association between peer attachment and two facets of grit in a sample of 964 participants. The results revealed that peer attachment contributes more to consistency of interests in adolescence and to perseverance of effort in emerging adulthood.