bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interleukin-13 receptor α2 is a novel marker and potential therapeutic target for human melanoma
Authors: Okamoto Hayato , Yoshimatsu Yasuhiro , Tomizawa Taishi , Kunita Akiko , Takayama Rina , Morikawa Teppei , Komura Daisuke , Takahashi Kazuki , Oshima Tsukasa , Sato Moegi , Komai Mao , Podyma-Inoue Katarzyna A. , Uchida Hiroaki , Hamada Hirofumi , Fujiu Katsuhito , Ishikawa Shumpei , Fukayama Masashi , Fukuhara Takeshi , Watabe Tetsuro 
Published: February 2019

AbstractMalignant melanoma is one of the untreatable cancers in which conventional therapeutic strategies, including chemotherapy, are hardly effective. Therefore, identification of novel therapeutic targets involved in melanoma progression is urgently needed for developing effective therapeutic methods. Overexpression of interleukin-13 receptor α2 (IL13Rα2) is observed in several cancer types including glioma and pancreatic cancer. Although IL13Rα2 is implicated in the progression of various types of cancer, its expression and roles in the malignant melanoma have not yet been elucidated. In the present study, we showed that IL13Rα2 was expressed in approximately 7.5% melanoma patients. While IL13Rα2 expression in human melanoma cells decreased their proliferation in vitro, it promoted in vivo tumour growth and angiogenesis in melanoma xenograft mouse model. We also found that the expression of amphiregulin, a member of the epidermal growth factor (EGF) family, was correlated with IL13Rα2 expression in cultured melanoma cells, xenograft tumour tissues and melanoma clinical samples. Furthermore, expression of amphiregulin promoted tumour growth, implicating causal relationship between the expression of IL13Rα2 and amphiregulin. These results suggest that IL13Rα2 enhances tumorigenicity by inducing angiogenesis in malignant melanoma, and serves as a potential therapeutic target of malignant melanoma.
N-formylation of amines using methanol as a potential formyl carrier by a reusable chromium catalyst
Authors: Yu Han , Wu Zhikang , Wei Zheyu , Zhai Yongyan , Ru Shi , Zhao Qixin , Wang Jingjing , Han Sheng , Wei Yongge 
Published: February 2019

AbstractFormamides represent an abundant class of compounds in organic synthesis. They can be made efficiently by the direct catalytic coupling of methanol with amines in the presence of metal-based catalysts. However, these catalysts require complicated organic ligands, susceptible to oxidative self-degradation, restricting their practical applications. Here, we describe an inorganic ligand-supported chromium (III) catalyst, (NH4)3[CrMo6O18(OH)6], which consists of a central chromium (III) single-atomic core supported by a cycle-shaped inorganic ligand consisting of six MoVIO6octahedra, shows excellent activity and selectivity. Various primary amines and secondary amines are successfully transformed into the corresponding formamides under mild conditions, and the formylation of primary diamines is also achieved. The chromium catalyst can be reused several times with little loss of the activity. Mechanistic insight is provided based on the observation of an intermediate and control experiments.
Functional analysis of the biochemical activity of mammalian phosphatidylinositol 5 phosphate 4-kinase enzymes
Authors: Mathre Swarna , Reddy K. Balasankara , Ramya Visvanathan , Krishnan Harini , Ghosh Avishek , Raghu Padinjat 
Published: February 2019

Abstract
Phosphatidylinositol 5 phosphate 4-kinase (PIP4K) are enzymes that catalyse the phosphorylation of phosphatidylinositol 5-phosphate (PI5P) to generate PI(4,5)P2. Mammalian genomes contain three genes, PIP4K2Α, 2B and 2C and murine knockouts for these suggested important physiological roles in vivo. The proteins encoded by PIP4K2A, 2B and 2C show widely varying specific activities in vitro; PIP4K2A is highly active and PIP4K2C 2000-times less active, and the relationship between this biochemical activity and in vivo function is unknown. By contrast, the Drosophila genome encodes a single PIP4K (dPIP4K) that shows high specific activity in vitro and loss of this enzyme results in reduced salivary gland cell size in vivo. We find that the kinase activity of dPIP4K is essential for normal salivary gland cell size in vivo. Despite their highly divergent specific activity, we find that all three mammalian PIP4K isoforms are able to enhance salivary gland cell size in the Drosophila PIP4K null mutant implying a lack of correlation between in vitro activity measurements and in vivo function. Further, the kinase activity of PIP4K2C, reported to be almost inactive in vitro, is required for in vivo function. Our findings suggest the existence of unidentified factors that regulate PIP4K enzyme activity in vivo.
Service and Learning at a Residential Parenting Program for Incarcerated Mothers: Speech-Language Pathology Student Outcomes and Maternal Perspectives
Authors: Pace Amy E. , Krings Kate , Dunlap Julie , Nehilla Lauren 
Published: February 2019

PurposeThis article reports preliminary outcomes from a service-learning (SL) experience for graduate students in the Speech-Language Pathology program with incarcerated mothers who reside with their infants at a residential parenting program. We present an ecological model to serve as a framework for interpreting the impact of the experience on student learning and maternal perceptions.MethodGraduate speech-language pathology students (totaln= 30) participated in an SL project, called thePartnership for Healthy Parenting. With faculty guidance, students implemented parent education and child development workshops designed to foster secure attachment relationships, enhance the quality of mother–child interactions, and promote communication and language development. Students completed self-report scales measuring civic attitudes and self-efficacy before participating; they also provided written reflections about their experience that were analyzed qualitatively. Mothers completed voluntary feedback surveys after each workshop.ResultsStudent reflections revealed a variety of perceived positive learning outcomes related to personal attitudes and beliefs about their role as a clinician, family-centered practices in early intervention, and knowledge about the population of interest. Feedback surveys administered to mothers who attended the workshops indicated satisfaction relating to the value of the services provided.ConclusionsSL programs may benefit students, faculty, communities, higher education institutions, and the relationships among all these stakeholders. Results, limitations, and implications for strengthening university–community collaborations in the field of communication disorders are discussed.
A new mandibular anchorage solution inspired from alpinism
Authors: Bernard-Granger C. , Cresseaux P. , Filippi R. 
Published: February 2019

In this article, we are presenting a new mandibular anchorage solution through three clinical cases.
This method is based on a mountain climbing technique. Thanks to a simple fixation wire insert in the jaw bone, it is now possible to obtain a backward movement of the mandibular arch and a significant uprighting of the incisors.
An original approach of maxillary transversal deficiency with lingual orthodontics
Authors: Bernard-Granger C. , Filippi R. , Cresseaux P 
Published: February 2019

The difficulty of management of a transverse maxillary hypoplasia is to choose the right treatment and the appropriate tools.
When transverse maxillary insufficient is associated to sagittal and/or vertical discrepancies in adults, the gold standard treatment is a surgical procedure combined with orthodontic treatment. The surgical procedure can be done in 1 or 2 stages.
If the patient chooses a lingual orthodontic technique, the tools for expansion and the stabilization of expansion are not simple to use.
The aim of this article is to report the case of a 25-year-old male patient, referred to our cabinet for skeletal Class-III malocclusion associated with laterognathism and transverse maxillary deficiency. The patient underwent one-stage surgery. He choose to be treated by a lingual orthodontic technique, we used the FKS® disjunction device.
Upper Back Pain among Physical Therapy Students and Its Association with Gender, Body Mass Index, Study Hours and Use of cell Phones
Authors: Fatima Sana , Arsh Aatik , Daud Muhammad , Gohar Mahreen , Ahmad Aftab , Shah Ali Maan 
Published: February 2019

Abstract
Introduction Musculoskeletal pain is common among Physical therapy students due to prolonged study hours and clinical practice. The objective of current study was to determine the prevalence of upper back pain among physical therapy students and its association with demographic information, length of study hours and use of cell Phones.
Methods This cross sectional survey was conducted in Peshawar, Pakistan from November 2017 to March 2018. 232 students from different physical therapy institutes were included in the study through consecutive sampling. Self-administered questionnaire was used as the study conducting tool having questions regarding demographic information, Body mass index (BMI) study hours, use of cell phone and upper back pain.
Results The Prevalence of upper back pain among DPT students was 44.8% (n=104) in which 33(31.7%) male students and 71(68.3%) female students had upper back pain. There was significant association between female gender and upper back pain (p-value<0.001). Nine (8.7%) underweight, 75 (72.1%) students with normal BMI, 18 (17.3%) overweight, and 2 (1.9%) obese students had upper back pain. There was no significant association between BMI and upper back pain (P=0.459). Majority of students (57.7%, n=60) who were involved in university activities like sitting looking straight during lectures on multimedia or white board for 3–5 h reported upper back pain. On the other hand majority of students (49%, n=51) whose study activities involve looking down during book reading etc for 1–2 h reported upper back pain. However, there was no significant association between activities like sitting looking straight (P=0.691) and sitting looking down (P=0.880) most of the time and upper back pain. Majority of students (43.3%, n=45) who used cell phone for more than 5 h had upper back pain and there was marginally significant association (P=0.091) between use of cell phones for long duration and upper back pain.
Conclusion The frequency of upper back pain was high among physical therapy students. Female gender was highly associated with upper back pain. There was no significant association of upper back pain with BMI, study hours and use of cell phones.
Neue Formen der Zusammenarbeit im ambulanten und stationären
Sektor: ein innovatives Förderkonzept
Authors: Schang Laura , Sundmacher Leonie , Grill Eva 
Published: February 2019

Zusammenfassung
Ziel der Studie Die Kontinuität der Versorgungs- und
Informationskette an der Schnittstelle zwischen stationärem und
ambulantem Sektor ist nicht immer gewährleistet. Um
Übergänge zwischen den Sektoren wie Aufnahme und Entlassung
aus der stationären Einrichtung zu verbessern und dem
Koordinationsbedarf von Patienten mit komplexen Erkrankungen oder multiplen
Risikofaktoren gerecht zu werden, wurden Eckpunkte für ein
Förderkonzept entwickelt.
Methodik Im Rahmen der Bayerischen Landesarbeitsgemeinschaft
Gesundheitsversorgungsforschung (LAGeV) wurden konzeptionelle Grundlagen
für die Förderung von sektorenübergreifenden
Kooperationen entwickelt.
Ergebnisse Das Konzept für neue Formen der Zusammenarbeit im
ambulanten und stationären Sektor umfasst die Förderung von
Strukturen intersektoraler Versorgungsnetzwerke und den modularen Ausbau
durch innovative Konzepte, die der patientenzentrierten Verbesserung der
Kommunikation und Koordination der Versorgung dienen. Dazu zählt
auch der Aufbau eines Case Managements zur Unterstützung der
Koordination an sektoralen Schnittstellen. Folgende Module werden vertiefend
erläutert: sektorenübergreifende, integrierte
Behandlungspfade; Entlassmanagement; intersektorale Qualitätszirkel;
strukturierte Pharmakotherapie; Einbindung von Akteuren aus der Pflege und
der Rehabilitation; und Gesundheitsförderung und Prävention.
Abschließend wird die Bedeutung einer systematischen und
vergleichenden Evaluation der Netzwerke erörtert.
Schlussfolgerung Damit wirksame Impulse für die Verbesserung
der sektorübergreifenden Kooperation gesetzt werden, sollten
zielgerichtete Module zur Verbesserung der patientenzentrierten Versorgung
an zentralen Schnittstelle zwischen stationärem und ambulantem
Sektor gefördert werden. Die verpflichtende Evaluation sollte
netzwerkübergreifend anhand validierter Qualitätsindikatoren
zur Prozess- und Ergebnisqualität als auch entlang
netzwerkspezifischen Zielen erfolgen.
Validität und Intratester-Reliabilität der Fotometrie zur Beurteilung der Position des Schultergürtels gesunder Probanden
Authors: Kapitza Camilla , Hoch Anke , Schöttker-Königer Thomas , Schäfer Axel 
Published: February 2019

Zusammenfassung
Hintergrund Da die Körperhaltung möglicherweise ein relevanter, beitragender als auch prognostischer Faktor bei muskuloskeletalen Problemen ist, stellt deren Beurteilung einen wichtigen Bestandteil der physiotherapeutischen Untersuchung dar. Das klinimetrische Verfahren der digitalen Fotometrie kann Parameter der Haltung objektiv erheben.
Ziel Die Studie untersuchte die Validität und die Intratester-Reliabilität eines Fotometrie-Programms zur Beurteilung der Güte seiner Anwendung in der Praxis und Forschung.
Methode In der Messgruppe wurde die Haltung des Schultergürtels von 48 Probanden im Alter von 19 bis 46 Jahren zu 2 Messzeitpunkten in 4 Ebenen gemessen, um folgende Variablen zu bestimmen: Skapula-Rotation, Pro- und Retraktion, Skapula-Kippung, kraniovertebraler Winkel, thorakale Kyphose und Schulterschiefstand. Die Intratester-Reliabilität wurde in einem Abstand von 3 Wochen untersucht. Die Bestimmung der Kriteriumsvalidität erfolgte über die Korrelation der Messwerte der Fotometrie mit denen eines digitalen Inklinometers, eines CROM und eines Zentimetermaßes.
Ergebnisse Validität: Die Korrelationskoeffizienten der gemessenen Winkel lagen zwischen r = 0,63, 95 %-Konfidenzintervall (KI) und r = 0,95, 95 %KI (p > 0,001), die Zentimetermessungen zwischen r = 0,57 und r = 0,96, 95 %KI (p < 0,0001). Intratester-Reliabilität: Die Intraklassenkorrelationskoeffizienten (ICC) der Winkelmessungen erreichten zwischen 0,77 und 0,96 CI und die ICC der Zentimetermessung zwischen 0,62 und 0,93 KI. Der Standardfehler der Messungen (SEM) betrug je nach Winkel zwischen 0,64° und 3,46° bzw. Abstand zwischen 0,35 cm und 0,98 cm.
Schlussfolgerung Die Ergebnisse lassen darauf schließen, dass die Fotometrie für die Winkel Skapula-Rotation, Skapula-Kippung und Akromioklavikulargelenk sowie für die Abstände zwischen Trigonum scapulae zur Wirbelsäule und Angulus inferior zur Wirbelsäule als valides und reliables Messinstrument zur Beurteilung des Schultergürtels bei gesunden Probanden verwendet werden kann. Insbesondere die Winkelmessungen zeigten gute Resultate.
Association between col1a2 Polymorphism and the Occurrence of Pelvic Organ Prolapse in Brazilian Women
Authors: Rosa Josyandra Paula de Freitas , Haddad Raphael Federicci , Maeda Fabiana Garcia Reis , Souto Ricardo Peres , Fernandes Cesar Eduardo , Oliveira Emerson de 
Published: February 2019

Objective To evaluate the rs42524 polymorphism of the procollagen type I alpha (α) 2 (COL1A2) gene as a factor related to the development of pelvic organ prolapse (POP) in Brazilian women.
Methods The present study involved 112 women with POP stages III and IV (case group) and 180 women with POP stages zero and I (control group). Other clinical data were obtained by interviewing the patients about their medical history, and blood was also collected from the volunteers for the extraction of genomic DNA. The promoter region of the COL1A2 gene containing the rs42524 polymorphism was amplified, and the discrimination between the G and C variants was performed by digestion of the polymerase chain reaction (PCR) products with the MspA1I enzyme followed by agarose gel electrophoresis analysis.
Results A total of 292 women were analyzed. In the case group, 71 had the G/G genotype, 33 had the G/C genotype, and 7 had the C/C genotype. In turn, the ratio in the control group was 117 G/G, 51 G/C, and 11 C/C. There were no significant differences between the groups.
Conclusion Our data did not show an association between the COL1A2 polymorphism and the occurrence of POP.
Challenges of Hearing Rehabilitation after Radiation and Chemotherapy
Authors: Nader Marc-Elie , Gidley Paul 
Published: February 2019

AbstractRadiation and chemotherapy are frequently used in the treatment of head and neck malignancies. Ototoxicity is a common adverse effect of these treatment modalities. This article discusses the patterns of hearing loss following chemotherapy and radiation therapy. Specific issues related to hearing rehabilitation in oncological patients will also be covered, such as controversies regarding treatment of radiation-induced serous otitis media, risks of otologic surgery, and the use of osseointegrated hearing aids and cochlear implants.
The constructionalization and constructional change of noncanonical V-NP expressions in Mandarin Chinese
Authors: Li Yanzhi , Wu Yicheng 
Published: February 2019

AbstractThis paper investigates the process of constructionalization and constructional change of apparently noncanonical yet highly frequent V-NP expressions in Mandarin Chinese, which exist in other languages as well (e.g., Southeast Asian languages and African languages). A corpus-based survey of 20 frequently used monosyllabic verbs shows that both the token and the type frequency of the noncanonical V-NP expressions have on the whole been increasing throughout the history of the Chinese language. From a constructional perspective, it is proposed that the noncanonical V-NP structure has gone through a process of grammatical constructionalization and constructional change (Traugott & Trousdale 2013), which involves diachronic changes in productivity, schematicity and compositionality and which is manifested in the increasing number of the semantic types of postverbal NPs.
The typological change of motion expressions in Chinese revisited
Authors: Shi Wenlei , Yang Wanglong , Su Henghua 
Published: February 2019

AbstractThis paper reports on a corpus-based study aimed at reexamining the typological status and diachronic change of motion expressions in Chinese, drawing on parallel texts consisting of autonomous motion expressions in Old Chinese (OC) and its Modern Chinese (MoC) translation. The results show that MoC significantly differs from OC both in the preference of lexicalization patterns (Talmyan typology) and semantic components distributed in discourse (Slobinian typology) when narrating similar motion scenes. However, these results fail to support the viewpoint that Chinese has undergone a change from a verb- to a satellite-frame (Li 1993;Talmy 2000;Peyraube 2006;Shi & Wu 2014). It is argued that (i) the Talmyan typology and the Slobinian typology should be treated separately. In Talmyan typology, the diachrony of Chinese demonstrates the change of a V- to a parallel-frame, in that satellite- and verb-framed constructions in MoC have equal frequency and show no bias for the encoding of subtypes of autonomous motion. In Slobinian typology, MoC remains as a Path-salient language, as it gives considerable weight to the expression of Path; (ii) the dominant lexicalization pattern in a language varies from one sub-domain of motion to another (see alsoLamarre 2003), and thus the typology of motion expressions is sub-domain-specific; and (iii) motivating forces and blocking forces, furthermore, co-exist diachronically for the typological evolution of motion encoding due to the idiosyncrasy of the morphosyntactic system.
Construction of a Pseudozyma antarctica strain without foreign DNA sequences (self-cloning strain) for high yield production of a biodegradable plastic-degrading enzyme
Authors: Sameshima-Yamashita Yuka , Watanabe Takashi , Tanaka Takumi , Tsuboi Shun , Yarimizu Tohru , Morita Tomotake , Koike Hideaki , Suzuki Ken , Kitamoto Hiroko 
Published: February 2019

ABSTRACT
The basidiomycetous yeast Pseudozyma antarctica GB-4(0) esterase (PaE) is a promising candidate for accelerating degradation of used biodegradable plastics (BPs). To increase safety and reduce costs associated with the use of PaE, we constructed a self-cloning strain with high-PaE productivity. A Lys12 gene (PaLYS12)-deleted lysine auxotroph strain GB4-(0)-L1 was obtained from GB-4(0) by ultraviolet mutagenesis and nystatin enrichment. Subsequently, the PaE gene (PaCLE1) expression cassette consisting of GB-4(0)-derived PaCLE1, under the control of a xylose-inducible xylanase promoter with PaLYS12, was randomly introduced into the GB4-(0)-L1 genome. A PaE high-producing strain, PGB474, was selected from among the transformants by high throughput double-screening based on its ability to degrade emulsified polybutylene succinate-co-adipate. Quantitative PCR revealed that four copies of the PaE gene expression cassette were introduced into the PGB474 genome. PGB474 produced 2.0 g/L of PaE by xylose-fed-batch cultivation using a 3-L jar fermentor for 72 h.
Ethyl Glucuronide as a Long-term Alcohol Biomarker in Fingernail and Hair. Matrix Comparison and Evaluation of Gender Bias
Authors: Paul R , Tsanaclis L , Murray C , Boroujerdi R , Facer L , Corbin A 
Published: February 2019

Abstract

Aims
This work aimed to assess the performance of hair and fingernail ethyl glucuronide (EtG) measurement for use as a biomarker of alcohol consumption in persons with known drinking history across a range of drinking behaviours.

Methods
EtG concentrations were assessed from the hair and fingernails of 50 study participants. Alcohol consumption of the previous 90 days was assessed by participant interview using the alcohol timeline follow-back method. EtG concentration was determined using LC–MS-MS using a method which was validated and accredited to ISO/IEC 17025 standards.

Results
There was significant correlation between alcohol consumption and EtG concentrations found in hair and fingernail samples across the study group (n = 50). From participants testing positive for EtG (male n = 14, female n = 13) no significant difference was found between male and female EtG levels in either hair or fingernails. Across all participants there was no significant difference in hair or fingernail EtG concentration between male (n = 23) and females (n = 27).

Conclusions
Our results support the use of EtG to indicate alcohol consumption over the previous 90 days, or ~3 months as is the normal practice in hair analysis. The results confirm that fingernails can be a useful alternative matrix where hair samples are not available.