bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the functional state of peach varieties (Prunus persica Mill.) when exposed hydrothermal stress to plants
Authors: Abilfazova Juliya , Belous Oksana 
Published: December 2018

The article presents data on the change in the ratio of pigments and fluorescence of chlorophyll in peach leaves in the optimal period of vegetation and under hydrothermal stress. When determining the resistance of a peach to unfavorable environmental factors, methods based on laboratory, fixed changes in the physiological and biochemical processes occurring in plants are used. In the period of inadequate water availability, the water deficit in peach leaves increased to an average of 15%, while less stable - an increase in the parameter to 18% was observed. It is shown that the xeromorphic character of the leaf apparatus is associated with a slight change in the anatomical characteristics of the leaf (the thickness of the leaf blade before and after the drought), which determines the permanence of the turgor. In this case, in the leaves of resistant varieties, the loss of turgor is insignificant (LSD (p ≤0.05) = 7.24); the thickness of the leaf fluctuates within 0.20 mm. On the background of stress impact, a clear varietal difference was shown, which allowed us to develop a scale for a comparative assessment of the resistance of peach varieties and clones. During the active growth of the assimilation surface, an increase in the amount of green pigments in the leaves of experimental plants was noted. Perspective varieties of peach contain significantly higher amounts of chlorophylls compared to other varieties (LSD (p ≤0.05) = 0.30). Under unfavorable conditions, in these varieties the ratio of the sum of chlorophylls to carotenoids is higher, which is confirmed by their more developed adaptive potential. Reorganization of the pigmentary apparatus during the period of hydrothermal stress is accompanied by an increase in the coefficient of photosynthetic activity (Kf_n) and a decrease in the fluorescence level (F_T) of chlorophyll. Thus, the water deficit, pigment composition and fluorescence of chlorophyll make it possible to identify the resistance of peach varieties and clones to the action of hydrothermal stressors. Based on the results of the studies, the most resistant varieties and clones of peaches have been identified for the humid subtropics of Russia (Larisa, Early bloy, Medin red, Slavutich, Donetskij zheltij, Vanity and Form 1).
Dose prescription with spatial uncertainty for peripheral lung SBRT
Authors: Bedford James L. , Blasiak‐Wal Irena , Hansen Vibeke N. 
Published: December 2018

AbstractCurrent clinical practice is to prescribe to 95% of the planning target volume (PTV) in 4D stereotactic body radiotherapy (SBRT) for lung. Frequently the PTV margin has a very low physical density so that the internal target volume (ITV) receives an unnecessarily high dose. This study investigates the alternative of prescribing to the ITV while including the effects of positional uncertainties. Five patients were retrospectively studied with volumetric modulated arc therapy treatment plans. Five plans were produced for each patient: a static plan prescribed to PTV D95%, three probabilistic plans prescribed to ITV D95% and a static plan re‐prescribed to ITV D95% after inverse planning. For the three probabilistic plans, the scatter kernel in the dose calculation was convolved with a spatial uncertainty distribution consisting of either a uniform distribution extending ±5 mm in the three orthogonal directions, a distribution consisting of delta functions at ±5 mm, or a Gaussian distribution with standard deviation 5 mm. Median ITV D50% is 23% higher than the prescribed dose for static planning and only 10% higher than the prescribed dose for prescription to the ITV. The choice of uncertainty distribution has less than 2% effect on the median ITV dose. Re‐prescribing a static plan and evaluating with a probabilistic dose calculation results in a median ITV D95% which is 1.5% higher than when planning probabilistically. This study shows that a robust probabilistic approach to planning SBRT lung treatments results in the ITV receiving a dose closer to the intended prescription. The exact form of the uncertainty distribution is not found to be critical.
Aerobic α‐Oxidation of N‐Substituted Tetrahydroisoquinolines to Dihydroisoquinolones via Organo‐photocatalysis
Authors: Aganda Kim Christopher C. , Hong Boseok , Lee Anna 
Published: December 2018

AbstractAn efficient visible‐light‐induced α‐oxidation of N‐substituted tetrahydroisoquinolines to dihydroisoquinolones has been developed using eosin Y as an organo‐photocatalyst and oxygen as a green oxidant. The reactions were carried out under mild reaction conditions; the desired dihydroisoquinolones were obtained in up to 96% yield at room temperature under oxygen atmosphere. This transformation provides a convenient route to dihydroisoquinolones with a wide range of substrates.magnified image
PiezoGRIN: A High‐Pressure Chamber Incorporating GRIN Lenses for High‐Resolution 3D‐Microscopy of living Cells and Tissues
Authors: Schneidereit Dominik , Schürmann Sebastian , Friedrich Oliver 
Published: December 2018

AbstractA high‐pressure optical chamber, PiezoGRIN, that facilitates label‐free 3D high‐resolution live‐cell multiphoton microscopy in thick tissue samples is presented. A set of two Gradient Index (GRIN) rod lenses is integrated into the chamber as an optical guide and allows for the adjustment of the focal plane through the sample providing a field of view volume of 450 × 450 × 500 µm (x, y, z). An optical lateral resolution of 0.8 µm is achieved by using two‐photon excitation with 150 fs pulses of a 810 nm titanium–sapphire laser at hydrostatic pressures up to 200 MPa. With the PiezoGRIN setup, it is possible to follow pressure‐induced changes in subcellular structure of unstained vital mouse skeletal muscle tissue up to 200 µm below the tissue surface.
Potential‐Induced Fine‐Tuning of the Enantioaffinity of Chiral Metal Phases
Authors: Assavapanumat Sunpet , Yutthalekha Thittaya , Garrigue Patrick , Goudeau Bertrand , Lapeyre Véronique , Perro Adeline , Sojic Neso , Wattanakit Chularat , Kuhn Alexander 
Published: December 2018

AbstractConcepts leading to single enantiomers of chiral molecules are of crucial importance for many applications, including pharmacology and biotechnology. Recently, mesoporous metal phases encoded with chiral information have been developed. Fine‐tuning of the enantioaffinity of such structures by imposing an electric potential is proposed, which can influence the electrostatic interactions between the chiral metal and the target enantiomer. This allows the binding affinity between the chiral metal and the target enantiomer to be increased, and thus, the discrimination between two enantiomers to be improved. The concept is illustrated by generating chiral encoded metals in a microfluidic channel by reduction of a platinum salt in the presence of a liquid crystal and l‐tryptophan as a chiral model template. After removal of the template molecules, the modified microchannel retains a pronounced chiral character. The chiral recognition efficiency of the microchannel can be fine‐tuned by applying a suitable potential to the metal phase. This enables the separation of both components of a racemate flowing through the channel. The approach constitutes a promising and complementary strategy in the frame of chiral discrimination technologies.
Impact of cell culture media additives on IgG glycosylation produced in Chinese hamster ovary cells
Authors: Ehret Janike , Zimmermann Martina , Eichhorn Thomas , Zimmer Aline 
Published: December 2018

AbstractGlycosylation is a key critical quality attribute for monoclonal antibodies and other recombinant proteins because of its impact on effector mechanisms and half‐life. In this study, a variety of compounds were evaluated for their ability to modulate glycosylation profiles of recombinant monoclonal antibodies produced in Chinese hamster ovary cells. Compounds were supplemented into the cell culture feed of fed‐batch experiments performed with a CHO K1 and a CHO DG44 cell line expressing a recombinant immunoglobulin G1 (IgG1). Experiments were performed in spin tubes or the ambr®15 controlled bioreactor system, and the impact of the compounds at various concentrations was determined by monitoring the glycosylation profile of the IgG and cell culture parameters, such as viable cell density, viability, and titer. Results indicate that the highest impact on mannosylation was achieved through 15 µM kifunensine supplementation leading to an 85.8% increase in high‐mannose containing species. Fucosylation was reduced by 76.1% through addition of 800 µM 2‐F‐peracetyl fucose. An increase of 40.9% in galactosylated species was achieved through the addition of 120 mM galactose in combination with 48 µM manganese and 24 µM uridine. Furthermore, 6.9% increased sialylation was detected through the addition of 30 µM dexamethasone in combination with the same manganese, uridine, and galactose mixture used to increase total galactosylation. Further compounds or combinations of additives were also efficient at achieving a smaller overall glycosylation modulation, required, for instance, during the development of biosimilars. To the best of our knowledge, no evaluation of the efficacy of such a variety of compounds in the same cell culture system has been described. The studied cell culture media additives are efficient modulators of glycosylation and are thus a valuable tool to produce recombinant glycoproteins.
DEPArray™ system: An automatic image‐based sorter for isolation of pure circulating tumor cells
Authors: Di Trapani Mariano , Manaresi Nicolò , Medoro Gianni 
Published: December 2018

AbstractCirculating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single‐cell level can inform on the genomic landscape of a tumor. All CTC enrichment methods bear a burden of contaminating normal cells, which mandate a further step of purification to enable reliable downstream genetic analysis. Here, we describe the DEPArray™ technology, a microchip‐based digital sorter, which combines precise microfluidic and microelectronic enabling precise, image‐based isolation of single CTCs, which can then be analyzed by Next Generation Sequencing (NGS) methods. © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
The effects of food level and social density on reproduction in the Least Killifish, Heterandria formosa
Authors: Leatherbury Kathryn N. , Travis Joseph 
Published: December 2018

AbstractThe feedbacks from population density to demographic parameters, which drive population regulation, are the accumulated results of several ecological processes. The compensatory feedback from increased population density to fertility includes at least two distinct factors, the effects of decreases in per capita food level and increases in the social density (the number of interacting individuals). Because these effects have been studied separately, their relative importance is unknown. It is also unclear whether food limitation and social density combine additively to influence fertility. We investigated these questions with two factorial experiments on reproduction in the Least Killifish, Heterandria formosa. In one experiment, we crossed two levels of density with two levels of a total food ration that was distributed to all individuals. In the other experiment, we crossed two levels of density with two levels of per capita food. Whereas the first experiment suggested that the effects of variation in food level and density were synergistic, the second experiment indicated that they were not. The apparent synergism—the statistical interaction of food and density levels—was the result of confounding per capita food with social density in that design. In the second experiment, the effects of social density on reproductive rate were stronger than the effects of food level, whereas the effects of food level were stronger on offspring size at parturition than those of social density. The results suggest that the social stresses that emerge at higher densities play an important role in the compensatory response of fertility to density, a role, that is, at least as important as that of decreased per capita food levels.
Responses of macroalgae to CO2 enrichment cannot be inferred solely from their inorganic carbon uptake strategy
Authors: van der Loos Luna M. , Schmid Matthias , Leal Pablo P. , McGraw Christina M. , Britton Damon , Revill Andrew T. , Virtue Patti , Nichols Peter D. , Hurd Catriona L. 
Published: December 2018

AbstractIncreased plant biomass is observed in terrestrial systems due to rising levels of atmospheric CO2, but responses of marine macroalgae to CO2 enrichment are unclear. The 200% increase in CO2 by 2100 is predicted to enhance the productivity of fleshy macroalgae that acquire inorganic carbon solely as CO2 (non‐carbon dioxide‐concentrating mechanism [CCM] species—i.e., species without a carbon dioxide‐concentrating mechanism), whereas those that additionally uptake bicarbonate (CCM species) are predicted to respond neutrally or positively depending on their affinity for bicarbonate. Previous studies, however, show that fleshy macroalgae exhibit a broad variety of responses to CO2 enrichment and the underlying mechanisms are largely unknown. This physiological study compared the responses of a CCM species (Lomentaria australis) with a non‐CCM species (Craspedocarpus ramentaceus) to CO2 enrichment with regards to growth, net photosynthesis, and biochemistry. Contrary to expectations, there was no enrichment effect for the non‐CCM species, whereas the CCM species had a twofold greater growth rate, likely driven by a downregulation of the energetically costly CCM(s). This saved energy was invested into new growth rather than storage lipids and fatty acids. In addition, we conducted a comprehensive literature synthesis to examine the extent to which the growth and photosynthetic responses of fleshy macroalgae to elevated CO2 are related to their carbon acquisition strategies. Findings highlight that the responses of macroalgae to CO2 enrichment cannot be inferred solely from their carbon uptake strategy, and targeted physiological experiments on a wider range of species are needed to better predict responses of macroalgae to future oceanic change.
Regio‐ and stereoselective addition of trans‐Pt(TeAr)(SiMe3)(PEt3)2 to terminal alkynes leading to cis‐[(Z)‐β‐trimethylsilylalkenyl]platinum(II) complexes
Authors: Han Li‐Biao , Mirzaei Farzad , Shimada Shigeru 
Published: December 2018

AbstractSelective insertion of terminal alkynes RC≡CH into the Si—Pt bond of trans‐Pt(TeAr)(SiMe3)(PEt3)2 took place readily to produce the corresponding novel alkenyl complexes cis‐Pt(TeAr)[(Z)‐CR=CHSiMe3](PEt3)2. The structure of the complex was characterized by an X‐ray analysis.
Smoking and papillomavirus DNA in patients with p16‐positive N3 oropharyngeal squamous cell carcinoma
Authors: Gorphe Philippe , Chekkoury Idrissi Younes , Tao Yungan , Moya‐Plana Antoine , Casiraghi Odile , Janot François , Blanchard Pierre , Mirghani Haïtham , Temam Stéphane 
Published: December 2018

AbstractBackgroundWe investigated the survival of patients with a p16‐positive N3 oropharyngeal squamous cell carcinoma (OPSCC) and the prognostic significance of patient, tumor, and treatment characteristics.MethodsWe retrospectively reviewed the data of patients treated at our Cancer Center for a p16‐positive N3 OPSCC between 2003 and 2016. End points were overall survival (OS) and progression‐free survival (PFS).ResultsA total of 29 patients were included. The 5‐year OS and PFS were 67.5% and 59.1%, respectively. Smoking history above 10 pack‐years and the absence of human papillomavirus DNA were associated with worse OS (P = .02 and P = .03, respectively) and PFS (P = .02 and P = .02, respectively). Induction chemotherapy or radical neck dissection were not associated with different treatment outcomes.ConclusionPatients with an N3 p16‐positive oropharyngeal cancer in our series had a 5‐year OS rate of 67.5%. Smoking history and viral DNA were prognostic factors associated with survival.
Ulcerative interstitial cystitis in an adolescent successfully treated with complete transurethral ulcer resection: A case report
Authors: Yuki Eri , Obara Kenji , Kuroki Hiroo , Yamazaki Hiroyuki , Yamana Kazutoshi , Tadokoro Akira , Hiruma Kaede , Tomita Yoshihiko 
Published: December 2018

IntroductionInterstitial cystitis is difficult to treat and may affect adolescents.Case presentationA 15‐year‐old girl presented with severe pain upon terminal micturition that persisted for approximately 2 hours. The pain had been present for more than 1 month. Cystoscopy revealed severe erosion throughout the trigone. Transurethral fulguration did not improve her symptoms. However, complete electric resection of the ulcer markedly reduced the symptom. After complete resection, pain on urination disappeared and she has had no pain without medication for 15 months.ConclusionComplete resection not fulguration of the ulcer is effective for interstitial cystitis in adolescent female patients.
Effects of laser shock peening on the corrosion behavior and biocompatibility of a nickel–titanium alloy
Authors: Zhang Ruixia , Mankoci Steven , Walters Nicholas , Gao Hongyu , Zhang Hao , Hou Xiaoning , Qin Haifeng , Ren Zhencheng , Zhou Xianfeng , Doll Gary L. , Martini Ashlie , Sahai Nita , Dong Yalin , Ye Chang 
Published: December 2018

AbstractNickel–titanium (NiTi) alloy is an attractive material for biomedical implant applications. In this study, the effects of laser shock peening (LSP) on the biocompatibility, corrosion resistance, ion release rate and hardness of NiTi were characterized. The cell culture study indicated that the LSP‐treated NiTi samples had lower cytotoxicity and higher cell survival rate than the untreated samples. Specifically, the cell survival rate increased from 88 ± 1.3% to 93 ± 1.1% due to LSP treatment. LSP treatment was shown to significantly decrease the initial Ni ion release rate compared with that of the untreated samples. Electrochemical tests indicated that LSP improved the corrosion resistance of the NiTi alloy in simulated body fluid, with a decrease in the corrosion current density from 1.41 ± 0.20 μA/cm2 to 0.67 ± 0.24 μA/cm2. Immersion tests showed that calcium deposition was significantly enhanced by LSP. In addition, the hardness of NiTi alloy increased from 226 ± 3 HV before LSP to 261 ± 3 HV after LSP. These results demonstrated that LSP is a promising surface modification method that can be used to improve the mechanical properties, corrosion resistance and biocompatibility of NiTi alloy for biomedical applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1854–1863, 2019.
Notoginsenoside R1 protects oxygen and glucose deprivation‐induced injury by upregulation of miR‐21 in cardiomyocytes
Authors: Liu Zengjia , Wang Haiyang , Hou Guoliang , Cao Honglei , Zhao Yan , Yang Baofa 
Published: December 2018

AbstractNotoginsenoside R1 (NG‐R1) is a major component of Panax notoginseng, which has been used clinically for the treatment of diabetic nephropathy for centuries in China. This study aimed to reveal the functional impacts and the underlying mechanisms of NG‐R1 on oxygen‐glucose deprivation (OGD)‐injured cardiomyocytes. Rat cardiomyocyte line H9c2 and primary cardiomyocytes were subjected to OGD with or without NG‐R1 treatment. The expression levels of miR‐21 and phosphatase and tensin homolog (PTEN) in the cell were altered by microRNA, vector or short‐hairpin RNA transfections. Thereafter, changes in cell viability, apoptosis, and PI3K/AKT signaling were monitored. NG‐R1 with low concentrations had no impact on H9c2 cells viability, but 80 μM of NG‐R1 significantly reduced cell viability. NG‐R1 (20 μM) protected H9c2 cells and primary cardiomyocytes against OGD‐induced cell damage, as cell viability was increased, apoptotic cell rate was reduced, and Bax, cleaved caspase‐3 and ‐9 were downregulated by addition of NG‐R1. MiR‐21 was low expressed in response to OGD exposure, while was highly expressed by NG‐R1 treatment. PTEN was a direct target of miR‐21. More interestingly, OGD‐induced cell damage could be recovered by miR‐21 overexpression or PTEN silence. Furthermore, PTEN silence recovered OGD‐blocked PI3K/AKT signaling pathway. To conclude, this study demonstrated that NG‐R1 exerted remarkable benefits in reduction of OGD‐induced cardiomyocyte loss. The cardioprotective actions of NG‐R1 possibly via upregulation of miR‐21, repressing the expression of miR‐21's target PTEN and thereby preventing the blockage of PI3K/AKT signaling pathway.
Physician referral to a psychologist: Testing alternative behavioral healthcare seeking models
Authors: Hammer Joseph H. , Spiker Douglas A. , Perrin Paul B. 
Published: December 2018

AbstractObjectivePrimary care physicians (PCPs) often refer patients to psychological services, but help‐seeking factors in the context of behavioral healthcare referral are understudied. This study examined perceptions of seeking psychological help for depression by comparing alternative structural equation models derived from the Theory of Reasoned Action (TRA).MethodInternet survey participants (N = 685 US adults, 77% female,
M
age = 45) imagined themselves in a vignette scenario in which they are experiencing depression symptoms and encouraged by a PCP to see a psychologist.ResultsResults supported the indirect model, in which the links between distal help‐seeking factors (i.e., self‐stigma, symptom recognition, perceived effectiveness of treatment) and intention to follow through on the referral to the psychologist were fully mediated by the more proximal TRA factors (i.e., attitudes, subjective norms, etc).ConclusionsOur findings supported the use of TRA in understanding peoples’ intention to seek psychological help for depression when referred by their PCP.