bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nonstationary environmental and community relationships in the North Pacific Ocean
Authors: Litzow Michael A. , Ciannelli Lorenzo , Puerta Patricia , Wettstein Justin J. , Rykaczewski Ryan R. , Opiekun Michael 
Published: May 2019

AbstractCommon approaches for summarizing multivariate environmental or community data assume that relationships among variables are stationary over time, and this assumption is often not tested. Here we test the hypothesis that relationships among environmental and community time series are nonstationary in the Gulf of Alaska ecosystem (North Pacific Ocean) over multidecadal time scales. Dynamic factor analysis (DFA) is applied to environmental and community data from before and after 1988/1989, corresponding to the timing of an abrupt decline in temporal variance of the Aleutian Low atmospheric pattern, a leading driver of Gulf of Alaska climate. Results show that covariance among local atmosphere and ocean environmental variables weakened simultaneous to the decline in Aleutian Low variance. At the same time, community‐wide responses of 14 fish and crustacean populations to physical forcing weakened, as indicated by nonstationary environment–biology regression coefficients. In line with theoretical predictions, this loss of a shared response to environmental variability was accompanied by weakening community covariance. Individual populations also showed nonstationary relationships with shared trends of community variability. We conclude that assumptions of fixed environmental and community relationships are likely to produce mistaken inference in this ecosystem. Similar concerns may apply in other ecosystems subject to changing climate patterns.
Acute heart failure congestion and perfusion status – impact of the clinical classification on in‐hospital and long‐term outcomes; insights from the ESC‐EORP‐HFA Heart Failure Long‐Term Registry
Authors: Chioncel Ovidiu , Mebazaa Alexandre , Maggioni Aldo P. , Harjola Veli‐Pekka , Rosano Giuseppe , Laroche Cecile , Piepoli Massimo F. , Crespo‐Leiro Maria G. , Lainscak Mitja , Ponikowski Piotr , Filippatos Gerasimos , Ruschitzka Frank , Seferovic Petar , Coats Andrew J.S. , Lund Lars H. ,   ESC‐EORP‐HFA Heart Failure Long‐Term Registry Investigators
Published: May 2019

AimsClassification of acute heart failure (AHF) patients into four clinical profiles defined by evidence of congestion and perfusion is advocated by the 2016 European Society of Cardiology (ESC)guidelines. Based on the ESC‐EORP‐HFA Heart Failure Long‐Term Registry, we compared differences in baseline characteristics, in‐hospital management and outcomes among congestion/perfusion profiles using this classification.Methods and resultsWe included 7865 AHF patients classified at admission as: ‘dry‐warm’ (9.9%), ‘wet‐warm’ (69.9%), ‘wet‐cold’ (19.8%) and ‘dry‐cold’ (0.4%). These groups differed significantly in terms of baseline characteristics, in‐hospital management and outcomes. In‐hospital mortality was 2.0% in ‘dry‐warm’, 3.8% in ‘wet‐warm’, 9.1% in ‘dry‐cold’ and 12.1% in ‘wet‐cold’ patients. Based on clinical classification at admission, the adjusted hazard ratios (95% confidence interval) for 1‐year mortality were: ‘wet‐warm’ vs. ‘dry‐warm’ 1.78 (1.43–2.21) and ‘wet‐cold’ vs. ‘wet‐warm’ 1.33 (1.19–1.48). For profiles resulting from discharge classification, the adjusted hazard ratios (95% confidence interval) for 1‐year mortality were: ‘wet‐warm’ vs. ‘dry‐warm’ 1.46 (1.31–1.63) and ‘wet‐cold’ vs. ‘wet‐warm’ 2.20 (1.89–2.56). Among patients discharged alive, 30.9% had residual congestion, and these patients had higher 1‐year mortality compared to patients discharged without congestion (28.0 vs. 18.5%). Tricuspid regurgitation, diabetes, anaemia and high New York Heart Association class were independently associated with higher risk of congestion at discharge, while beta‐blockers at admission, de novo heart failure, or any cardiovascular procedure during hospitalization were associated with lower risk of residual congestion.ConclusionClassification based on congestion/perfusion status provides clinically relevant information at hospital admission and discharge. A better understanding of the clinical course of the two entities could play an important role towards the implementation of targeted strategies that may improve outcomes.
Low complexity SVM technique of control implementation by microcontroller for three phase solar inverter
Authors: Tayebi Azzedinne , Brahami Mostefa , Yaichi Mohammed , Boutadara Abdelkader 
Published: May 2019

AbstractThis article presents a method for simplifying the programming and implementation of the space vector modulation (SVM) algorithm, applied to the prototype of the three‐phase inverter for stand‐alone photovoltaic pumping system using induction motor. In this system, the control is usually based on V/f constant approach. It is known that the SVM approach is fully with mathematical formulas which complicate its implantation. The purpose of this work is to reduce both the SVM algorithm complexity and program size, which becomes about 2.55Ko, and this achievement done based on two procedures: the first, must fixes the location of angular set points on the trajectory of the reference voltage vector. The second is precalculation of the switching times and that is make it feasible and simple due to the technique V/f constant ratio. The proposed method makes the SVM algorithm implementation in the microcontroller PIC18F4550 more easier and tested in the real time platform to control the three legs inverter feeding an induction motor as load. The simulation and experimental results of the proposed method are presented and compared with the theoretical results of conventional SVM. The obtained results are very satisfactory for this kind of application.
Determining consumer's carbon emission obligation through virtual emission tracing in power systems
Authors: Rouholamini Mahdi , Miller Carol J. , Wang Caisheng 
Published: May 2019

AbstractAlthough a recent report by the U.S. Energy Information Administration indicates that CO2 emissions from power generation have now fallen below emissions from the transportation sector, electricity use remains a significant part of human's environmental footprint since fossil fuels are the major energy resource for electricity generation worldwide. This explains the continual effort by policy makers to develop a remedy to mitigate carbon emissions. It is then critically important to properly account for the emissions associated with electricity generation, delivery, and consumption. In particular, it is critical to be able to allocate emissions to the relevant electricity consumers creating the demand that results in a polluting emission. Therefore, in this paper a virtual carbon emission tracing method in power systems is thoroughly presented to facilitate allotting carbon obligation. The method is developed based on the proportional sharing principle that has been used in power system operation. The proposed methodology is implemented on the IEEE 5‐bus and 9‐bus systems, two widely used and recognized power grid benchmarks.
Spatiotemporal variations of dissolved inorganic carbon and controlling factors in a small karstic catchment, Southwestern China
Authors: Qin Caiqing , Li Si‐Liang , Yue Fu‐Jun , Xu Sheng , Ding Hu 
Published: May 2019

AbstractDissolved inorganic carbon (DIC) is the most important carbon component in karst aquatic system where fluid is highly transmissive, but has rarely been examined in the subtropical karst critical zone (K‐CZ). In this study, concentrations of dissolved solutes and isotopic compositions of DIC (δ13CDIC) at 11 sites of a 73.4 km2karstic catchment in Southwestern China were analysed monthly in order to uncover the spatiotemporal variations of both DIC and its dominant sources, and to identify relevant controlling factors. Both DIC concentrations and δ13CDICwere highly variable, ranging from 2.52 to 5.85 mmol l−1and from −15.7 to −4.5‰, respectively. DIC in underground water (UGW) was higher in concentration and more depleted in13C compared to surface water (SFS). DIC concentrations showed an inconsistent seasonal trend with other solutes, with higher values in the wet season at some sites. δ13CDICvalues were lower in the wet season than in the dry season. The results of mixing model IsoSource revealed spatiotemporal patterns of DIC sources. During the dry season, carbonate weathering was the primary contributor to DIC in UGW (excluding in the middle reaches). However, during the wet season, soil CO2was the dominant source of DIC in both UGW and SFS, and it was higher than in the dry season. Overall, there are significant spatiotemporal disparities and highly transmissive characteristics of both DIC and its sources in the K‐CZ, which are controlled by multiple factors. This study also highlights that rainfall may play a crucial role in accelerating carbon dynamics in the K‐CZ. High‐frequency sampling campaigns in high‐flow periods and deep analyses are needed in future work to elucidate the related processes and mechanisms. © 2019 John Wiley & Sons, Ltd.
Fabrication of Composite Hydrogels Based on Soy Protein Isolate and their Controlled Globular Protein Delivery
Authors: He Naipu , Chen Xiunan , Wang Li , Wen Jing , Li Yuhong , Cao Qi , Liu Zaiman , Li Baiyu 
Published: May 2019

AbstractSoy protein isolate (SPI) protein/polymer composite hydrogels (PPCGs) are fabricated in a urea solution of SPI using acrylic acid as monomer, ammonium persulphate (APS) as initiator, and N,N‐methylenebisacrylamide (BIS) and glutaraldehyde (GA) as cross‐linking agents. The scanning electron microscope (SEM) results show that SPI/polyacrylic (PAA) composite hydrogels formed network structure. In particular, in the absence of cross‐linking agent (GA), the network structure of composite hydrogels is also formed by BIS cross‐linking chains of PAA and the hydrophobic interactions between peptides from SPI and chain of PAA. In addition, composite hydrogels have good water absorption and present excellent pH sensitivity. Composite hydrogels adsorb bovine serum albumin (BSA) with higher adsorption capacity. BSA is the control released in pH 7.4 buffers and the accumulative release ratio achieved is 90%. It will be expected that these protein/polymer composite hydrogels could be applied for drug sustained release materials.
Exploring women's preferences for HPV‐based cervical cancer screening in South Africa
Authors: Oberlin Austin M. , Pasipamire Tafadzwa , Chibwesha Carla J. 
Published: May 2019

AbstractObjectiveTo determine preferences for HPV‐based cervical cancer screening among South African women.MethodsA discrete choice experiment survey was conducted among 298 women who attended two public‐sector clinics in South Africa from February 1 to May 31, 2018. Participants chose between hypothetical screening scenarios: method of swab collection (self or provider); timing of treatment (same day or return visit); type of clinic (static or mobile); cost of services (US $0 or US $4); and time spent at the clinic (30, 60, or 120 minutes). A logistic regression model was generated to evaluate the importance of each attribute. A market simulation analysis was performed to determine potential uptake of the various screening strategies.ResultsThe participants expressed strong preferences for free services (β=0.50; P<0.001) and same‐day HPV testing and treatment (β=0.40; P<0.001). The market simulation indicated that 83.8% of women would be willing to undergo screening if services were free; the swab was collected by the provider; and treatment was offered at a return visit. Including same‐day testing and treatment in the model increased uptake to 96.4%.ConclusionOffering same‐day HPV testing and treatment could substantially improve uptake of cervical cancer screening in the South African public healthcare sector.
Wip1 cooperates with KPNA2 to modulate the cell proliferation and migration of colorectal cancer via a p53‐dependent manner
Authors: Wang Peng , Zhao Yahui , Liu Kuijie , Liu Xianghe , Liang Jianwei , Zhou Haitao , Wang Zheng , Zhou Zhixiang , Xu Ningzhi 
Published: May 2019

AbstractDue to the increasing incidence and mortality, the early diagnosis, specific targeted therapies, and prognosis for colorectal cancer (CRC) attract more and more attention. Wild‐type p53‐induced phosphatase 1 (Wip1) and karyopherin α2 (KPNA2) have been regarded as oncogenes in many cancers, including CRC. Wip1 dephosphorylates p53 to inactivate it. TP53 activator and Wip1 inhibitor downregulate KPNA2 expression. Therefore, we speculate that Wip1 may co‐operate with KPNA2 to modulate CRC progression in a p53‐dependent manner. Here, Wip1 and KPNA2 messenger RNA expression and protein levels are significantly increased in CRC tissues and cell lines and are positively correlated with each other. Wip1 silence increases p53 phosphorylation while decreases KPNA2 protein. Wip1 knockdown remarkably suppresses CRC cell proliferation and migration while KPNA2 overexpression exerts an opposing effect. KPNA2 overexpression could partially rescue Wip1 silence‐inhibited CRC cell proliferation and migration. Finally, Wip1 interacts with KPNA2 to modulate the activation of AKT/GSK‐3β signaling and metastasis‐related factors. In summary, Wip1 could co‐operate with KPNA2 to modulate CRC cell proliferation and migration, possibly via a p53‐dependent manner, through downstream AKT/GSK‐3β pathway. We provided a novel mechanism of Wip1 interacting with KPNA2, therefore modulating CRC cell proliferation and migration.
Role of heterogeneous nuclear ribonucleoprotein K in tumor development
Authors: Chen Yuting , Zeng Ying , Xiao Zheng , Chen Shi , Li Yukun , Zou Juan , Zeng Xi 
Published: May 2019

AbstractHeterogeneous nuclear ribonucleoprotein K (hnRNP K) is an RNA/DNA special binding protein that participates in regulating the expression of related genes, transcription, RNA alternative splicing, translation, posttranslational modification, cell signal transduction, cell movement, interacts with ncRNAs, and induces angiogenesis. Moreover, several cellular functions forcefully indicated that hnRNP K participates in tumorigenesis. Numerous studies indicated hnRNP K is aberrantly elevated in multiple tumors. In addition, hnRNP K abnormal accumulation in cytoplasmic is also associated with poor prognosis. This suggests that hnRNP K may play a role in the development and progression of tumors. However, related studies demonstrated that hnRNP K acts as a tumor suppressor to suppress tumor formation. Therefore, this paper aims to explore the role of hnRNPK in tumors.
The miRNA hsa‐miR‐6515‐3p potentially contributes to lncRNA H19‐mediated‐lung cancer metastasis
Authors: Xu YouZu , Lin Jian , Jin YingYing , Chen Meifang , Zheng HaiHong , Feng JiaXi 
Published: May 2019

AbstractAberrant expression of long noncoding RNAs (lncRNAs) contributes to all phenotypes of cancer including metastasis, which is a major cause of death in many advanced malignancies. One particular lncRNA, H19, is found to be a crucial player in cancer progression by modulating multiple microRNAs (miRNAs). In this study, we screened miRNAs possibly associated with H19 using lung carcinoma cell lines and patient with lung cancer tissues, and selected one possible hit, hsa‐miR‐6515‐3p, to perform in vitro functional assays. Its inhibition leads to decreased proliferation and migration of SPC‐A1 lung cancer cells and is in good correlation with H19‐knockdown groups. These results indicate that H19 may be an epigenetic regulator of miR‐6515‐3p, and its dysregulation may contribute to lung cancer progression and metastasis.
Comprehensive analysis of differentially expressed profiles of lncRNAs, mRNAs, and miRNAs in laryngeal squamous cell carcinoma in order to construct a ceRNA network and identify potential biomarkers
Authors: Kong Xinru , Qi Jixia , Yan Yao , Chen Liwei , Zhao Yali , Fang Zhongju , Fan Junda , Liu Mingbo , Liu Yehai 
Published: May 2019

AbstractObjectiveThis study aimed to uncover a regulatory network comprised of long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) in laryngeal squamous cell carcinoma (LSCC), to explore its underlying mechanisms and development, and to identify key genetic biomarkers for the prognosis of LSCC.MethodsHere, we compared mRNA, lncRNA, and miRNA expression profiles between 111 LSCC and 12 adjacent normal tissues using RNA sequencing (RNA‐Seq) data from the Cancer Genome Atlas (TCGA) database. Based on the interaction information obtained from miRcode, TargetScan, miRTarBase, and miRDB, a lncRNA–miRNA–mRNA competing endogenous RNA (ceRNA) network was constructed using differentially expressed lncRNAs (DElncRNA), miRNAs (DEmiRNA), and mRNAs (DEmRNA). By assessing the functional enrichment of DEmRNAs in this network, the potential underlying mechanisms were explored. In addition, Kaplan–Meier survival analysis was used to assess genetic biomarkers related to the prognosis of LSCC patients.ResultsUpon comparing LSCC and control tissues, 1640 DElncRNAs, 75 DEmiRNAs, and 3217 DEmRNAs were identified. Based on the prediction between lncRNA–miRNA and miRNA–mRNA relationships, we constructed a ceRNA network comprised of 93 lncRNAs, nine miRNAs, and nine mRNAs. This network predicted that two lncRNAs (AC016773.1 and C00299), two mRNAs (DIO1 and STC2), and two miRNAs (hsa‐mir‐137 and hsa‐mir‐210) were significant biomarkers of LSCC prognosis according to thorough topological and survival analyses (P < .05).ConclusionThrough a ceRNA network analysis, our study identifies new lncRNAs, miRNAs, and mRNAs, which can be used as potential biomarkers of LSCC and as therapeutic targets for treating LSCC, thus laying a foundation for future clinical studies.
CircRNA expression profile of bovine placentas in late gestation with aberrant SCNT fetus
Authors: Su Xiaohu , Gao Guangqi , Wang Shenyuan , Su Guanghua , Zheng Zhong , Zhang Jiaqi , Han Lidong , Ling Yu , Wang Xiuying , Li Guangpeng , Zhang Li 
Published: May 2019

AbstractBackgroundsOne of the limitations of somatic cell nuclear transfer (SCNT) strategy to generate genetically modified offspring is the low birth rate. Placental dysfunction is one of the causes of abortion. Circular RNA (circRNA) is noncoding RNA which functions as microRNA (miRNA) sponges in biological processes.MethodsTwo aberrant pregnant placenta (aberrant group, AG) and three normal pregnant placenta (normal group, NG) during late gestation (180‐210 days) with bovine SCNT fetus were collected for high‐throughput sequencing and analyzed. The host genes of differentially expressed (DE) circRNAs were predicted. And the microRNAs (miRNAs) which could interact with DE circRNAs were analyzed. Then, the expressional level of partial DE circRNAs and corresponding host genes was verified through qRT‐PCR. At last, the function of host genes was analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).ResultsAltogether 123 differentially expressed circRNAs between two groups were identified, which were found related to 60 host genes and 32 miRNAs. The top 10 upregulated circRNAs were bta_circ_0012985, bta_circ_0013071, bta_circ_0013074, bta_circ_0016024, bta_circ_0013068, bta_circ_0008816, bta_circ_0012982, bta_circ_0013072, bta_circ_0019285, and bta_circ_0013067. The top 10 downregulated circRNAs were bta_circ_0024234, bta_circ_0017528, bta_circ_0008077, bta_circ_0003222, bta_circ_0007500, bta_circ_0020328, bta_circ_0011001, bta_circ_0016364, bta_circ_0008839, and bta_circ_0016049. The qRT‐PCR results showed consistent trend with sequencing analysis result, while host genes had no statistic difference. The GO and KEGG analyses of the host genes suggested that abnormal circRNA expression may play multiple roles in placental structure and dysfunction.ConclusionThe abnormal circRNA expression may be one of reasons of placental dysfunction, leads to abortion of bovine SCNT fetus.
CRNDE enhances neuropathic pain via modulating miR‐136/IL6R axis in CCI rat models
Authors: Zhang Dawei , Mou Jun‐Ying , Wang Fang , Liu Juan , Hu Xue 
Published: May 2019

AbstractNeuropathic pain has been reported as a type of chronic pain due to the primary dysfunction of the somatosensory nervous system. It is the most serious types of chronic pain, which can lead to a significant public health burden. But, the understanding of the cellular and molecular pathogenesis of neuropathic pain is barely complete. Long noncoding RNAs (lncRNAs) have recently been regarded as modulators of neuronal functions. Growing studies have indicated lncRNAs can exert crucial roles in the development of neuropathic pain. Therefore, our present study focused on the potential role of the lncRNA Colorectal Neoplasia Differentially Expressed (CRNDE) in neuropathic pain progression. Firstly, a chronic constrictive injury (CCI) rat model was built. CRNDE was obviously increased in CCI rats. Interestingly, overexpression of CRNDE enhanced neuropathic pain behaviors. Neuroinflammation was induced by CRNDE and as demonstrated, interleukin‐10 (IL‐10), IL‐1, IL‐6, and tumor necrosis factor‐α (TNF‐α) protein levels in CCI rats were activated by LV‐CRNDE. For another, miR‐136 was obviously reduced in CCI rats. Previously, it is indicated that miR‐136 participates in the spinal cord injury via an inflammation in a rat model. Here, firstly, we verified miR‐136 could serve as CRNDE target. Loss of miR‐136 triggered neuropathic pain remarkably via the neuroinflammation activation. Additionally, IL6R was indicated as a target of miR‐136 and miR‐136 regulated its expression. Subsequently, we confirmed that CRNDE could induce interleukin 6 receptor (IL6R) expression positively. Overall, it was implied that CRNDE promoted neuropathic pain progression via modulating miR‐136/IL6R axis in CCI rat models.
Modulation of macrophage subtypes by IRF5 determines osteoclastogenic potential
Authors: Yang Jihyun , Park Ok‐Jin , Kim Jiseon , Kwon Yeongkag , Yun Cheol‐Heui , Han Seung Hyun 
Published: May 2019

AbstractBone‐resorbing osteoclasts are differentiated from macrophages (MΦ) by M‐CSF and RANKL. MΦ can be mainly classified into M1 and M2 MΦ, which are proinflammatory and anti‐inflammatory, respectively, but little is known about their osteoclastogenic potential. Here, we investigated the osteoclastogenic potential of MΦ subtypes. When the two MΦ subtypes were differentiated into osteoclasts using M‐CSF and RANKL, M2 MΦ more potently differentiated into osteoclasts than M1 MΦ. M2 MΦ generated with IL‐4 or IL‐10 also showed enhanced osteoclast differentiation compared with M1 MΦ induced by IFN‐γ and lipopolysaccharide. In addition, robust bone‐resorptive capacity and giant actin rings, which are features of mature osteoclasts, were observed in M2, but not M1 MΦ, under the osteoclast differentiation condition. Osteoclast differentiation was significantly increased in CD206+ M2 MΦ but not in CD86+ M1 MΦ. Compared with M1 MΦ, c‐Fms and RANK were highly expressed in M2 MΦ. Enhanced osteoclastogenesis of M2 MΦ was mediated through sustained ERK activation, followed by efficient c‐Fos and NFATc1 induction. Notably, the osteoclastogenic potential of M1 MΦ converted into M2 MΦ by exposure to M‐CSF was higher than that of M2 MΦ converted into M1 MΦ by exposure to GM‐CSF. Silencing IRF5, which is responsible for M1 MΦ polarization, increased osteoclast differentiation by enhancing c‐Fms expression and activation of ERK, c‐Fos, CREB, and NFATc1, which was inhibited by overexpression of IRF5. Collectively, M2 MΦ are suggested to be more efficient osteoclast precursors than M1 MΦ because of the attenuated expression of IRF5.