bluuebunny/crossref_metadata_2025_split huggingface dataset:
Down-regulated miR-145 rescues insulin resistance in endothelial cells
Authors: Wang Jing , Dong Zhichun , Lou Liying 
Published: September 2019

Abstract
Background: MiR-145 is involved in insulin resistance (IR) in liver cells, but its effects in human umbilical vein endothelial cells (HUVECs) induced by IR remains unclear. This study took this as the starting point, aiming to find a potential target for the treatment of related disease.
Methods: HUVECs were respectively treated with glucose of 15, 30, 45 mmol/L, or insulin of 1, 2, 3, 4, 5 μmol/L on the basis of high-glucose (33.3 mmol/L). MiR-145 mimics and miR-145 inhibitor were severally transfected into HUVECs with or without IR (4 μmol/L insulin + high-glucose). Quantitative real-time polymerase chain reaction (qRT-PCR) assay determined the miR-145 expression in HUVECs after treatment and transfection. The glucose consumption and glycogen contents of cells were appraised by glucose oxidase-peroxidase and anthranone-sulfuric acid methods, respectively. The apoptotic rates were ascertained using the flow cytometry. The expressions of apoptosis-related indicators Bcl-2 and Bax were analyzed by western blot (WB) and qRT-PCR assays.
Results: The expression of miR-145 was increased in IR models and incremental glucose concentrations. The glucose consumption and glycogen content were down-regulated in IR-induced HUVECs, which were enhanced by over-expressed miR-145 but reversed by down-regulation. Moreover, over-expression of miR-145 aggravated the apoptosis of IR-induced HUVECs, while the inhibition of miR-145 had a completely opposite effect. Accordingly, up-regulated miR-145 obviously reduced Bcl-2 level and enhanced Bax expression in IR models, which was contrary to the down-regulated miR-145.
Conclusion: Down-regulated miR-145 rescued IR in endothelial cells, which might be a conceivable treatment for IR of endothelial cells.
Serum platelet-derived growth factor is a novel biomarker for the prediction of vulnerable plaque in patients with NSTE-ACS
Authors: Zhang Hong , Zhang Ying , Liu Yujie , Ma Kejing , Zhou Jia , Guan Jingjing 
Published: September 2019

Abstract
Objective: To investigate the relationship between serum platelet-derived growth factor (PDGF) and vulnerable plaque in patients with non-ST-elevation acute coronary syndrome (NSTE-ACS).Methods: A total of 65 patients with NSTE-ACS were divided into vulnerable plaque group (n=46) and vulnerable plaque and stable plaque group (n=19) according to intravascular ultrasound (IVUS) examinations. Total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and serum PDGF were measured. Plaque characteristics and components were analyzed using gray-scale and iMap-IVUS. Correlation was performed between plaque characteristics and ACS markers. Logistic regression analysis was applied to determine risk factors. Receiver operating characteristic (ROC) curve was used to evaluate the predictive value.Results: Patients in vulnerable plaque group had visible higher levels of TG, LDL-C and PDGF (P < 0.05). There were significant differences in minimal lumen area (MLA), plaque area, plaque burden, fibrotic (FI), clipidic (LI) and necrotic core (NC) between the two groups (P < 0.05). PDGF was weakly correlated with plaque burden (R=0.428, P < 0.05), as well as moderately correlated with NC (R=0.669, P < 0.05). Multivariate analysis showed that serum PDGF (OR 4.751, [95% CI 1.534-29.543], P=0.05) was an independent risk factor of vulnerable plaque. The area under the curve (AUC) was 0.876 (95% CI 0.804-0.948, P=0.001).Conclusion: Serum PDGF could be used as a novel biomarker for the prediction of vulnerable plaque in patients with NSTE-ACS.
BRD9 controls Oxytocin signal pathway in gastric cancer via CACNA2D4 and CALML6
Authors: Wang Yuan , Jiang Xue-Yan , Zheng Guo-Dong , Xu Chuan-Shan , Liang Lu , Lin Zhong-Xiao , Yu Xi-Yong 
Published: September 2019

Abstract
Background: First-line chemotherapeutic agents have great activation treatment in cancers, but the side effect of these kind drugs also hurt the healthy cells, in some cases, cancer cells are induced drug resistance to chemotherapeutic agents. The molecular mechanisms underlying such side effect have been studied in a range of cancer types, yet little is known in depressing the adverse effect of chemotherapeutic drugs by targeting BRD9 in gastric cancers. Methods: We used two gastric cancer cell lines (MGC-803 and AGS) for comparison. We applied molecular and cellular techniques to measure cell survival, mRNA expression, investigated clinical data in the consensus of The Cancer Genome Atlas, and combined with the High-Throughput Sequencing approach in MGC-803 cells and AGS cells, for global gene expression analysis in inhibiting BRD9 conditions. Results: Our studies have shown that cancer cells with BRD9 over-expression, MGC-803 cells, are more sensitive to BRD9 inhibitors, BI9564 or BI7273, than AGS cells. Its mechanism is related to the regulation of CACNA2D4 and CALML6 oncogenes in the Oxytocin signaling pathway. And BRD9 inhibitors can enhance the sensitivity of gastric cancer MGC-803 cells to adriamycin and cisplatin, so we may reduce the dosage of chemotherapeutic agents in curing gastric cancers with BRD9 over-expression by combining BI9564 or BI7273 with adriamycin or cisplatin. Conclusions: Our study elucidates the feasibility and effectiveness of inhibiting BRD9 to reduce the adverse effect of first-line chemotherapeutic agents in treating over-expressed BRD9 gastric cancer, providing a scientific theoretical basis on chemotherapy regimen in over-expressed BRD9 gastric cancer.
A systematic review of parental vaccine hesitancy and refusal in childhood vaccination
Authors: Dyda Amalie , King Catherine , Dey Aditi , Leask Julie , Dunn Adam 
Published: September 2019

Abstract
Background Acceptance of vaccines is an important predictor of vaccine uptake. This has public health implications as those who are not vaccinated are at a higher risk of infection from vaccine preventable diseases. We aimed to systematically review studies of parental attitudes and beliefs in childhood vaccination, with a focus on the methods used to measure hesitancy and refusal.Methods We identified and reviewed primary research studies using quantitative methods and investigating vaccine attitudes and beliefs published between January 2012 and May 2018. Studies were included if they involved a quantitative survey of the attitudes and beliefs of parents about vaccinations recommended for children. We undertook a narrative synthesis of the results with a focus on evaluating variation in the use of behavioural theories, validated survey instruments, and localisation and adaptation of questions to suit local context.Results A total of 116 studies met the inclusion criteria; 99 used a cross sectional study design, 5 used a case control study design, 4 used a pre-post study design and 8 used mixed methods study designs. Sample sizes of included studies ranged from 49 to 12,259. Thirty-six countries were represented in the included studies. The most commonly used tool was the Parent Attitudes about Childhood Vaccines (PACV) Survey. Questions eliciting vaccination attitudes and beliefs varied widely.Conclusions There was heterogeneity in the types of measures used in studies investigating attitudes and beliefs about vaccination in parents. Broader and more consistent use of validated survey instruments for measuring parental attitudes and beliefs about childhood vaccination would help to better understand localised differences in the reasons for vaccine hesitancy and refusal.
The Sydney Triage to Admission Risk Tool (START) to improve patient flow in an Emergency Department: A model of care implementation pilot study
Authors: Ebker-White Anja , Bein Kendall , Russell Saartje Berendsen , Dinh Michael 
Published: September 2019

Abstract
Background The Sydney Triage to Admission Risk Tool (START) is a validated clinical analytics tool designed to estimate the probability of in-patient admission based on Emergency Department triage characteristics.Methods This was a single centre pilot implementation study using a matched case control sample of patients assessed at ED triage. Patients in the intervention group were identified at triage by the START tool as likely requiring in-patient admission and briefly assessed by an ED Consultant. Bed management were notified of these patients and their likely admitting team based on senior early assessment. Matched controls were identified on the same day of presentation if they were admitted to the same in-patient teams as patients in the intervention group and same START score category. Outcomes were ED length of stay and proportion of patients correctly classified as an in-patient admission by the START tool.Results One hundred and thirteen patients were assessed using the START-based model of care. When compared with matched control patients, this intervention model of care was associated with a significant reduction in ED length of stay [301 minutes (IQR 225-397) versus 423 minutes (IQR 297-587) p<0.001] and proportion of patients meeting four hour length of stay thresholds increased from 24% to 45% (p<0.001).Conclusion In this small pilot implementation study, the START tool, when used in conjunction with senior early assessment was associated with a reduction in ED length of stay. Further controlled studies are now underway to further examine its utility across other ED settings.
MiPepid: MicroPeptide identification tool using machine learning
Authors: Zhu Mengmeng , Gribskov Michael 
Published: September 2019

Abstract
Background

Micropeptides are small proteins with a length of <= 100 amino acids. Short open reading frames that could produces micropeptides were traditionally ignored due to technical difficulties, as few small peptides had been experimentally demonstrated. In the past decade, a growing number of micropeptides have been shown to play significant roles in vital biological activities. Despite the increased amount of data, we still lack bioinformatics tools for specifically identifying micropeptides from DNA sequences. Indeed, most existing tools for classifying coding and noncoding ORFs were built on datasets in which “normal-sized” proteins were considered to be positives and short ORFs were generally considered to be noncoding. Since the functional and biophysical constraints on small peptides are likely to be different from those on “normal” proteins, methods for predicting short translated ORFs must be trained independently from those for longer proteins.Results

In this study, we have developed MiPepid, a machine-learning tool specifically for the identification of micropeptides. We trained MiPepid using carefully cleaned data from existing databases and used logistic regression with 4-mer features. With only the sequence information of an ORF, MiPepid is able to predict whether it encodes a micropeptide with 96% accuracy on a blind dataset of high-confidence micropeptides, and to correctly classify newly discovered micropeptides not included in either the training or the blind test data. Compared with state-of-the-art coding potential prediction methods, MiPepid performs exceptionally well, as other methods incorrectly classify most bona fide micropeptides as noncoding. MiPepid is alignment-free and runs sufficiently fast for genome-scale analyses. It is easy to use and is available at https://github.com/MindAI/MiPepid.Conclusions

MiPepid was developed to specifically predict micropeptides, a category of proteins with increasing significance, from DNA sequences. It shows evident advantages over existing coding potential prediction methods on micropeptide identification. It is ready to use and runs fast.
keywords: micropeptide, small ORF, sORF, smORF, coding, noncoding, lncRNA, machine learning
Randomizowane badania kliniczne w protonoterapii– jedyna słuszna droga?
Authors: Konkol Marek 
Published: September 2019

Protonoterapia jest szybko rozwijającą się nową technologią radioterapii onkologicznej. W aktualnej debacie podkreśla się brak wystarczającej ilości randomizowanych badań klinicznych (RCTs, randomized controlled trials) do udowodnienia jej klinicznej wyższości. Ta praca podsumowuje bieżący stan wiedzy oraz polemizuje z koniecznością używania RCTs do oceny protonoterapii.
Nowotworowe komórki krążące: biologia i znaczenie kliniczne
Authors: Suchorska Wiktoria 
Published: September 2019

W ostatnich latach co raz większe zainteresowanie wzbudza tzw. płynna biopsja (ang. liquid biopsy) polegająca na analizie tzw. krążących komórek nowotworu (ang. circulating tumor cells, CTC) i /lub fragmentów DNA (ang. circilating tumor DNA, ctDNA) pochodzących z komórek nowotworowych w krwi obwodowej. CTC to komórki, które przeniknęły z guza pierwotnego do światła naczyń krwionośnych i są przenoszone w organizmie w krążeniu krwi. Uważa się, że komórki te stanowią źródło rozwoju choroby przerzutowej, a ich wykrywanie i analiza może mieć znaczenie w prognozowaniu przebiegu rozwoju nowotworu. CTC zostały po raz pierwszy opisane już w 1869 roku przez Thomasa Ashwortha, który postulował, że „komórki te obecne w krwi obwodowej, identyczne z komórkami guza, mogą tłumaczyć obecność wielu guzów tego samego typu u jednego chorego”. Ostatnie badania potwierdzają przydatność oznaczania liczby komórek krążących nowotworu jako narzędzia prognostycznego i markera postępu choroby.
Yeni Bir Sosyal Bilim Paradigmasına Doğru: Mirasın Reddi ve Bütünsel Bilim Arayışları
Authors: Özkurt Cem 
Published: September 2019

Bir
bilgi inşa etme tarzı olarak sosyal bilimin kimliği, 19. yüzyılda doğa
bilimleri/ kültür ve beşeri bilimler ayrımının epistemolojik / metodolojik
saflaşmasının ürünü olarak ortaya çıktı ve bu saflaşmada iyiyi vaaz etme yetkesini
sosyal bilimler üstlenirken, doğruyu
vaaz etme yetkesi ise doğa bilimlerine bırakıldı. İlk kertede, sosyal bilimler
ve doğa bilimlerinin doğru ile iyi arasındaki bu bilimsel taksimatı kabul edilebilir
gözüktü. Gerçekte ise, iyinin ve doğrunun birbirlerinden kopuk ve izole edilmiş olarak
çıktıkları tarihsel yolculukta, hem sosyal bilimler hem de doğa bilimleri,
iktidar ve güç yapılarıyla aralarındaki
ahlaki, vicdani ve bilimsel mesafeyi korumakta başarısız oldular ve araçsal aklın egemenliğinde hem doğrunun
hem de iyinin saflığı yapı bozumuna uğradı. En genel çerçevede
değerlendirildiğinde, bilimler, özgürlük idealini betimleyebilecekleri bir konum almaktan çok uzaklaştılar.
Bu makale, sosyal bilimler ve doğa
bilimlerinin, doğru ile iyi arasında inşa
ettikleri dikatominin biçimlendirdiği iki kültürlü mirasın krizlerine ve
epistemolojik bunalımlarına özellikle
vurgu yapmaktadır. Çıkış yolları çerçevesinde ise, bilimlerde parçalılığın
reddi ve bütünsel bilim arayışlarını çağdaş yaklaşımlar ve güncel tartışmalar
bağlamında betimlemeyi amaçlamaktadır.
The Effect of Metformin on Thyroid-Associated Serum Hormone Levels and Physiological Indexes: A Meta-Analysis
Authors: Wang Junjie , Gao Jinghan , Fan Qin , Li Hongzhuo , Di Yunhua 
Published: September 2019

Background:
Many diseases can be treated with metformin. People with serum thyrotropin (TSH)
levels higher than 10 mIU/L are at a risk of cardiovascular events. Some studies have suggested that metformin
can lower serum TSH levels to a subnormal level in patients with hyperthyrotropinaemia or hypothyroidism.

Objective:
The objective of this analysis is to evaluate the effect of metformin treatment on serum TSH, free
triiodothyronine (FT3), and free thyroxine (FT4) levels and other associated physiological indices.

Methods:
A comprehensive search using the PubMed, EMBASE, Web of Science and Cochrane Central databases
was undertaken for controlled trials on the effect of metformin on serum TSH, FT3, and FT4 levels and
associated physiological indices. The primary outcome measures were serum TSH, FT3 and FT4 levels, thyroid
size, thyroid nodule size, blood pressure, heart rate, body weight, and body mass index (BMI). The final search
was conducted in April 2019.

Results:
Six RCTs were included. A total of 494 patients met the inclusion criteria. Metformin treatment did not
significantly lower the serum TSH levels at 3 or 6 months but did at 12 months. Moreover, forest plots also suggested
that metformin can significantly lower the serum TSH levels in patients with normal thyroid function but
cannot statistically change the serum TSH levels in patients with abnormal thyroid function. In addition, metformin
treatment clearly lowered the serum FT3 levels and had no significant effect on serum FT4 levels. Lastly,
metformin cannot significantly change the systolic blood pressure (SBP) or BMI but can clearly increase the
diastolic blood pressure (DBP).

Conclusion:
Metformin treatment can significantly lower the serum TSH levels, and this effect was much clearer
after a 12-month treatment duration and in people with normal thyroid function. However, metformin cannot
significantly change the serum FT4 levels or lower serum FT3 levels in people with non-thyroid cancer diseases.
In addition, metformin can significantly increase DBP, but it has no clear effect on SBP or BMI.
Synergism Effects of Ursolic Acid Supplementation on the Levels of Irisin, C-reactive Protein, IL-6, and TNF-α During High-intensity Resistance Training in Low Activity Men
Authors: Asghari Ehsan , Rashidlamir Amir , Hosseini Seyyed R.A. , Moazzami Mahtab , Samarghandian Saeed , Farkhondeh Tahereh 
Published: September 2019

Background::
Ursolic Acid (UA) is a pentacyclic triterpenoid carboxylic acid which is
extracted from plants. UA may enhance the effect of Resistance Training (RT) in human.

Objective:
Current research was designed to show the effect of High-Intensity Resistance Training
(HIRT) in the presence or absence of UA on the serum levels of irisin, CRP, IL-6 and TNF-α in the
low activity men.

Method::
The study included twenty-two healthy male HIRT with placebo, supplementation, and
HIRT in the presence of UA supplementation. The two groups received eight-week intervention
including 2 sets of 8 exercises, with 8~10 repetitions at 70~75% of 1 repetition maximum and a 2
min rest interval between sets, performed 3 times/week. Placebo or UA orally was evaluated as 1
capsule 3 times/day during 8 weeks. The subsequent factors were measured post- and preintervention:
C-Reactive Protein (CRP), Irisin, Tumor Necrotic Factor (TNF-α) and Interleukin-6 (IL-6).

Results::
UA supplementation significantly increased the plasma levels of irisin in the HIRT+UA
group versus the HIRT+P group (p<0.05). UA treatment also dramatically decreased the plasma
levels of CRP, IL-6, and TNF-α in the HIRT+UA group versus the HIRT+P group (p<0.05).

Conclusion::
The current data showed that UA-induced an increase in serum irisin and reduction of
CRP, IL-6, and TNF-α may have beneficial effects as a chemical for increasing of the effects of
HIRT in low activity men.
The Simplified of Suspended Sediment Measurement Method in Natural River (Case study of Kuning River in Yogyakarta, Indonesia)
Authors: Yulistiyanto Bambang , Kironoto Bambang , Giarto Bangun , Kiptiah Mariatul , Tantowi Muhammad Lutfi 
Published: September 2019

The accumulation of suspended sediment reduces the capacity in the river and deteriorates the water quality. Kuning River in Yogyakarta is one of the main rivers in Yogyakarta, Indonesia, which is currently facing the issue of suspended sediments. To reduce the effect of suspended sediment and determine a preventive measure, hence, it is necessary to study the characteristics of the suspended sediment flow. Therefore, this study aims to investigate the suspended sediment flow characteristics, i.e. the velocity, and the concentration profiles at specific points in the transverse direction of the channel as well as the correlation of the suspended sediment discharge. Thirty (30) profiles of velocity and suspended sediment concentration were measured at six different points along the Kuning River. Opcon probe was used to measure suspended sediment concentration, while the propeller current meter was used to measure mean point-velocity profiles. Results of this study show the suspended sediment discharge ratio, defined as are higher in the middle part of the channel than the one near the edge of the channel. The position of z/B where the values of 1 occurs at z/B = 0,19 and z/B = 0,75, which depend on the irregularity of the channel cross-sections. For practical purposes, the depth-averaged velocity and suspended sediment concentration can be determined from 1, 2 and/or 3 points measurement at y = 0,2D, 0,4D and 0,8D.
O COMBATE DA CASTIDADE: AUTONOMIA E EXERCÍCIO DA SEXUALIDADE ENTRE HOMENS EVANGÉLICOS COM PRÁTICAS HOMOSSEXUAIS
Authors: Natividade Marcelo 
Published: September 2019

Este artigo problematiza a elaboração de identidades pessoais em face
das complexas constelações culturais contemporâneas. Considerando que existem
múltiplos discursos relativos à homossexualidade, reflete-se sobre a produção
de identidades sexuais de sujeitos com pertença religiosa. Parte-se do suposto
de que processos de construção de si estão imbricados a questões de gênero,
inserção social e clivagens como religião e orientação sexual. Primeiramente,
reflete-se sobre as possíveis correlações entre percursos afetivo-sexuais e trajetórias
religiosas. A partir disso, são analisados discursos sobre a experiência de buscar
“cura” na esfera da sexualidade, focalizando narrativas sobre a participação em
rituais de libertação e aconselhamentos pastorais. O material etnográfico consiste
em entrevistas realizadas entre os anos de 2001 e 2005, entre sujeitos de distintas
denominações pentecostais no universo evangélico, residentes em regiões da
Baixada Fluminense e Zona Norte do Rio de Janeiro.
ENSINO RELIGIOSO EM ESCOLAS PÚBLICAS NO BRASIL: NOTAS DE PESQUISA
Authors: Giumbelli Emerson 
Published: September 2019

O texto procura discutir algumas dimensões da definição e implementação da
disciplina de ensino religioso em escolas públicas no Brasil. Inicia com reflexões mais gerais
que levam em consideração a noção de laicidade e os marcos nacionais para o ensino religioso.
Em seguida, propõe um cotejo entre as situações dos estados de São Paulo e Rio de Janeiro,
abordando contrastes e pontos convergentes. Por fim, apresenta os primeiros resultados de uma
pesquisa que cobre 12 estados brasileiros, destacando três dimensões: os aparatos estatais, os
conselhos inter-religiosos e a formação de professores.
EDUCAÇÃO E RELIGIÃO: O NOVO MODELO DE ENSINO RELIGIOSO NAS ESCOLAS PÚBLICAS DO ESTADO DO RIO GRANDE DO SUL
Authors: Júnior Cesar A. Ranquetat 
Published: September 2019

O presente texto procura examinar, o processo de implementação do novo modelo de
ensino religioso nas escolas públicas do estado do Rio Grande do Sul, em obediência à Lei
Federal 9.475/97. Demonstra o predomínio de igrejas cristãs, principalmente a Igreja Católica
na organização e administração do ensino religioso e a persistência, no Rio Grande do Sul, de
práticas confessionais e proselitistas contrastantes com a proposta legal de uma disciplina de
ensino religioso não-confessional e pluralista.
Algumas palavras sobre a Filosofia na Educação Básica
Authors: Godoy Evandro Carlos 
Published: September 2019

A Filosofia, a partir de 2012, determina a Resolução nº 1/2009 da Câmara de Educação Básica do Conselho Nacional de Educação, deve estar incluída no currículo de todas as séries do Ensino Médio em todas as escolas do país. Na perspectiva de que este retorno não seja apenas pela obrigação legal, mas pela efetiva necessidade e valor dos conhecimentos filosóficos, apresentamos neste texto argumentos em favor do retorno da Filosofia ao currículo escolar. Pretendemos aqui, sobretudo, divulgar e compartilhar algumas reflexões iniciais sobre o ensino de Filosofia na Educação Básica. Reflexões que passam pelas pretensões que a Filosofia pode ter na escola, oferecendo justificativas e evidenciando o valor do ensino de Lógica e da História da Filosofia.