bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical performance of the HPV DNA Array genotyping assay in detection of CIN2+ lesions with BS GP5+/6+ MPG Luminex tested cervical samples
Authors: Pesic Aleksandra , Krings Amrei , Hempel Matthias , Preyer Rosemarie , Kaufmann Andreas M. 
Published: August 2019

AbstractHuman papillomavirus (HPV) detection is used for screening of cervical cancer and genotype‐specific persistence has shown to be mandatory for dysplasia development. Aim of this study was to evaluate the clinical performance of HPV DNA Array for cervical intraepithelial neoplasia 2+ (CIN2+)  lesion detection. HPV DNA Array is a polymerase chain reaction‐based assay that targets E1 sequences of 29 HPV types (6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 44, 45, 51, 52, 53, 54, 56, 58, 59, 66, 67, 68, 69, 70, 73, 82, 85, and 97). The clinical evaluation was performed against the reference assay, BS‐GP5+/6+ multiplex genotyping (MPG)‐Luminex, with 600 cervical smear samples of a referral population. HPV DNA Array detected CIN2+  lesions with a sensitivity of 90.2%, identical to that of MPG‐Luminex. Detection of CIN3+  lesions was with a sensitivity of 90.3%, as compared with 88.7% of MPG‐Luminex. It demonstrated very good agreement for HPV detection, irrespective of type, of 91.5% (κ  =  0.832). HPV DNA Array is a simple and robust assay, with a short protocol of 4 hours hands‐on time and automated readout by ELISpot AiDot software. It permits testing of up to 96 samples in one run and may be considered for use in organized screening programs and low resource settings.
High glucose disrupts autophagy lysosomal pathway in gingival epithelial cells via ATP6V0C
Authors: Huang Xin , Kuang Shuhong , Shen Zongshan , Liang Min , Lin Zhengmei 
Published: August 2019

AbstractBackgroundHyperglycemic micro‐environment induced by diabetes could regulate the response of periodontal tissues to pathogenic microorganisms in which disruption of autophagy lysosomal pathway (ALP) may participate. This study aimed to explore the mechanisms underlying how high glucose (HG) regulates ALP in gingival epithelial cells (GECs).MethodsHuman gingival tissues in healthy group (C), periodontitis group (P), diabetes group (DM), and diabetes + periodontitis group (DP) were collected and were applied to observe the fusion of autophagy and lysosome. Diabetic mouse model with periodontitis was established and RNA‐seq was applied to investigate the expression of ALP‐associated genes in gingival epithelium. To explore the key role of ATPase transmembrane v0 domain, subunit c (ATP6V0C) in the disruption of ALP by HG, human gingival epithelial cells (HGECs) were cultured in 5.5 mM/25 mM glucose medium for 48 hours and followed by Porphyromonas gingivalis stimulation for 0, 6, and 12 hours. HBLV‐h‐ATP6V0C was transfected in HGECs that were stimulated by 25 mM HG condition.ResultsImmunofluorescence double staining exhibited the disruption of ALP in human gingival epithelium in diabetes groups and HGECs under 25 mM glucose condition, accompanied with significantly downregulated lysosomal acidity. RNA‐seq of mouse gingival epithelium screened out Atp6v0c. Compared with HGECs in normal culture medium, ATP6V0C expression and LC3‐II/I expression ratio were significantly downregulated, with an upregulated expression of P62, IL‐1β in HGECs under HG condition. Over‐expression of ATP6V0C rescued HG‐induced disruption of ALP in HBLV‐h‐ATP6V0C transfected HGECs, with significantly upregulation of LC3‐II/I and downregulation of P62, IL‐1β.ConclusionATP6V0C mediates HG‐induced ALP disruption in HGECs, eventually exacerbates periodontal inflammation.
The use of nanotechnology to increase quality and yield of fruit crops
Authors: Zahedi Seyed Morteza , Karimi Mahdieh , Teixeira da Silva Jaime A 
Published: August 2019

AbstractNanotechnology is currently being widely employed in agriculture and horticulture. The most widely investigated and developed factor related to fruit trees is nanofertilizers (NFs), which play very important roles in increasing vegetative growth, improving reproductive growth and flowering, thereby increasing productivity, product quality and ultimately increasing shelf‐life and decreasing fruit waste. These nanomaterials, which are generally sprayed at low concentrations on trees at different time intervals and in frequent sessions, are also considered as growth stimulants. Macro‐ and micro‐scale NFs such as zinc, boron, chitosan, and fertilizer nanocomposites such as ZnFeMnB (zinc, iron, manganese, boron), NPKMg (nitrogen, phosphorus, potassium, magnesium), and calcite have been shown to significantly improve the vegetative and reproductive traits of fruit trees such as pomegranate, strawberry, mango, date, coffee and grape. Knowledge on the effects of NFs on fruit trees and biological reasons for their effects on different traits is incomplete and there is an urgent need for extensive research on these topics. © 2019 Society of Chemical Industry
Hydroxycinnamic acids and human health: recent advances
Authors: Coman Vasile , Vodnar Dan C 
Published: August 2019

AbstractThere is an urgent need to improve human diet globally. Compelling evidence gathered over the past several decades suggests that a suboptimal diet is associated with many chronic diseases and may be responsible for more deaths than any other risks worldwide. The main components in our diet that need higher intake are whole grains, fruit and vegetables, and nuts and seeds; all of these are important sources of dietary fiber and polyphenols. The health benefits of dietary fiber and polyphenols are also supported by several decades of valuable research. However, the conclusions drawn from interventional human trials are not straightforward and the action mechanisms in improving human health are not fully understood. Moreover, there is a great inter‐individual variation caused by different individual capabilities of processing, absorbing and using these compounds effectively. Data on the bioavailability and bioefficacy of hydroxycinnamic acids (HCAs) are limited when compared to other classes of polyphenols (e.g. anthocyanins). This review aims to summarize the latest research advances related to HCA bioavailability and their biological effects revealed by epidemiological data, pre‐clinical and clinical studies. Moreover, we aim to review the effects of HCAs on gut microbiota diversity and function and its respective influence on host health. © 2019 Society of Chemical Industry
Evaluation of the feasibility of short‐term electrodialysis for separating naturally occurring fluoride from instant brick tea infusion
Authors: Peng Chuan‐yi , Liu Hai‐feng , Qiao Huan‐huan , Luo Jing , Liu Xi‐min , Hou Ru‐yan , Wan Xiao‐chun , Cai Hui‐mei 
Published: August 2019

AbstractBACKGROUNDRemoving excessive naturally occurring fluoride from tea and/or infusions is difficult because the process has low efficiency and causes secondary pollution. In this study, a novel electrodialysis (ED) technology was developed. We examined the effect of crucial parameters (electrolyte concentration, operation voltage, ED duration and initial concentration of the tea infusion) on defluoridation performance using a highly efficient ion‐exchange membrane with five‐compartment cells.RESULTSThe most effective ED system results were obtained at an electrolyte concentration of 10 g kg−1 and operating voltage of 20 V. Moreover, the fluoride removal capacity (10.70–66.93%) was highly dependent on the ED duration (1–15 min) and initial concentration of the tea infusion (0.5–10 g kg−1). The longer the ED duration and the lower the initial concentration, the higher was the defluoridation performance. During ED, limited loss of the main inclusions (total polyphenols, catechins, caffeine and selected ions) was observed. Furthermore, the D201 anion resin‐filled ED stack (0.5–5 g) and improvement of concentrate compartment electrolyte (≥5 times the dilute compartment electrolyte) in the ED system enhanced the defluoridation rate significantly.CONCLUSIONED is a potentially effective method that can be used for defluoridation in the deep processing of tea products. © 2019 Society of Chemical Industry
Quality characteristics of bean paste as a confectionery ingredient and recent breeding efforts of common beans in Japan
Authors: Sadohara Rie 
Published: August 2019

AbstractBean paste is a confectionery ingredient originating in Asia made from cooked beans and sugar. In Japan, bean paste‐containing products play an important role in the traditional confectionery industry. Common beans (Phaseolus vulgaris L.) are used for making white bean paste, and the tebou market class is dedicated to white paste production. Bean paste qualities include paste yield, color, stickiness, smoothness, aroma and flavor. High paste yield, whiteness and smoothness are preferred. The ideal stickiness depends on the final product to be made using bean paste. In terms of aroma and flavor, high sweetness and low beaniness are generally desired. Most of the paste qualities can only be measured by preparing bean paste, which is labor intensive and low throughput. Yuki and Kinu tebou bean varieties were developed in this manner because the highest end‐use quality is indispensable to domestic varieties. Tebou bean breeding in Japan is at the stage where more research is necessary to develop faster screening methods to predict important paste quality attributes. This review summarizes the literature on research on white bean paste quality and common bean breeding efforts conducted so far written either in English or Japanese, covering: (1) bean paste production and ingredient sources, (2) the selection criteria and methods used by Japanese breeders and (3) the resulting varieties developed for bean paste. © 2019 Society of Chemical Industry
Cooperative effects of slight acidic electrolyzed water combined with calcium sources on tissue calcium content, quality attributes, and bioactive compounds of 'Jiancui' jujube
Authors: Li Xiaojuan , Zhi Huanhuan , Li Meng , Liu Qiqi , Xu Juan , Dong Yu 
Published: August 2019

AbstractBACKGROUNDSlight acidic electrolyzed water (SAEW) has been widely used in cleaning systems of fruit and vegetables. It strongly reduces microbial contamination. However, no information is available on whether SAEW offers the potential for fresh jujube cleaning. The purposes of this study were, first, to compare the effectiveness of SAEW with commercial sanitizers (i.e. sodium hypochlorite (NaClO) or calcium hypochlorite (Ca(ClO)2)) on 'Jiancui' jujube; second, to determine the response of fruit decay, tissue calcium (Ca) content, and quality attributes to dip application of calcium nitrate (Ca(NO3)2) by concentration; and third, to investigate the effects of SAEW combined with Ca(NO3)2 or calcium acetate (Ca(OAc)2)) on fruit Ca uptake, quality attributes, and bioactive compounds.RESULTSFruits washed with NaClO, Ca(ClO)2, or an SAEW solution showed no difference in reduction of decay incidence. In contrast to NaClO treatment, SAEW or Ca(ClO)2 significantly retarded losses in fruit firmness (FF), green color (hue angle), and skin lightness (L*), and maintained intact pericarp tissue structure during storage at 1 °C. Application of Ca(NO3)2 at 5–10 g L−1 effectively promoted Ca2+ uptake and minimized declines in FF and L* value but had no effect on decay development. Adding 10 g L−1 Ca(NO3)2 or Ca(OAc)2 to SAEW provided an additional benefit in increasing decay resistance, increasing Ca2+ into fruit and increasing levels of bioactive compounds in jujube fruit.CONCLUSIONSAEW in combination with Ca(NO3)2 or Ca(OAc)2 has commercial potential for fresh jujube disinfection and improving storage quality as a result of the cleaning processes. © 2019 Society of Chemical Industry
Polytetrafluoroethylene granuloma‐associated facial palsy following microvascular decompression
Authors: Derakhshan Adeeb , Greene Jacqueline J. , Gadkaree Shekhar K. , Chen Jenny X. , Jowett Nate , Hadlock Tessa A. 
Published: August 2019

ObjectiveMicrovascular decompression (MVD) may be employed in the management of hemifacial spasm (HFS), wherein a pledget of polytetrafluoroethylene (i.e., Teflon, Chemours, Wilmington DE) is sometimes introduced to separate an offending vessel from the cisternal segment of facial nerve. Rarely, Teflon may cause a granulomatous reaction resulting in nerve palsy. We here present the first case series of facial palsy thought to be secondary to Teflon granuloma following MVD for HFS.MethodsA data repository of 1,312 patients with facial palsy was reviewed to identify individuals who had previously undergone MVD for HFS. Data collected include age at time of MVD, age at onset of facial weakness and at presentation, House‐Brackmann scores, clinician‐graded facial function using the Electronic Facial Paralysis Assessment scale, imaging findings, and therapeutic interventions and outcomes.ResultsSix patients meeting criteria were identified. Average time between MVD with Teflon placement and onset of facial weakness was 16.1 (±4.9) years (range 9.3–23.3 years). Initial House‐Brackmann scores were as follows: four patients with V/VI and one each with III/VI and IV/VI. Interventions included eyelid weight placement (n = 3), chemodenervation (n = 2), static suspension with tensor fascia latae (n = 2), dynamic reanimation with cranial nerves V to VII transfer (n = 1), and temporalis muscle transfer (n = 1).ConclusionTeflon granuloma should be considered in the differential diagnosis for patients presenting with new onset facial weakness with a previous history of MVD for HFS. It remains unknown whether early granuloma extirpation is effective. Prompt diagnosis allows consideration of time‐sensitive nerve transfer procedures to reanimate facial function.Level of Evidence4 Laryngoscope, 130:1422–1427, 2020
Seedling and ramet recruitment at the community level on recent landslide scars on the hilly and gullied Loess Plateau, China
Authors: Hu Shu , Jiao Juying , Li Yujin , Deng Na , Wu Duoyang , Zhang Yifeng 
Published: August 2019

AbstractIn this study, we aimed to provide a comprehensive insight into seedling and ramet recruitment at the community level on recent landslide scars (i.e., the sliding surfaces regarded as the most difficult zones of landslides to restore), which may cast new light on landslide ecology and enhance theoretical support for interventions on landslide restoration. Through seedling and ramet monitoring and adult surveying on 12 three‐year‐old landslide scars on the hilly and gullied Loess Plateau, China, we characterized seedling and ramet recruitment in terms of their trade‐offs, processes and amounts, and we also assessed compositional correspondence between the adult and young (all seedlings and ramets combined) layers. We found that when seedling recruitment was employed in tandem with ramet recruitment, which might maximize colonists' fitness. Moreover, seedlings and ramets emerged constantly and were followed by low mortality rates. However, there were problems of emergence limitation and constant death, and, as a result, seedlings and ramets remained at low densities. Therefore, there appeared to be a slow and limited recruitment process of plant colonists on the scars. In addition, there was a high degree of compositional uncoupling between the adult and young layers, implying future potential changes of plant communities driven by Poa sphondylodes Trin., Stipa bungeana Trin., Ixeridium sonchifolium (Maxim.) Shih, Astragalus discolor Bunge ex Maxim. and Artemisia scoparia Waldst. et Kit. on shady scars and I. sonchifolium (Maxim.) Shih on sunny scars. We also proposed that the first three species can be used to restore landslide scars in the study area.
Vegetation type and plant diversity affected soil carbon accumulation in a postmining area in Shanxi Province, China
Authors: Yan Meifang , Cui Feifei , Liu Yang , Zhang Zeyu , Zhang Jianbiao , Ren Hongrui , Li Zhiping 
Published: August 2019

AbstractCoal mining causes severe land degradation, which poses a great damage to carbon (C) sequestration function of the original ecosystem. Vegetation restoration in postmining areas plays an important role in rebuilding soil C pool and improving soil quality. In this study, soil C sequestration and soil properties of different vegetation types were investigated along restoration chronosequences in a postmining area in the eastern Loess Plateau. The results showed that soil properties improved substantially through artificial restoration by plantingRhus typhina(exotic species) andPlatycladus orientalis(indigenous species) and through natural restoration. Soil C stock (0–20 cm depth) increased significantly with restoration duration in all of the vegetation types and was positively correlated to plant diversity except inR. typhina. After 12–20 years from restoration, accumulation rates of soil C varied significantly with vegetation type, with the maximum inP. orientalis(0.60 Mg ha−1yr−1) and the minimum in naturally restored grassland (0.21 Mg ha−1year−1). The optimum performance ofP. orientalisplantation could be attributed mainly to high plant diversity and associated high fine root biomass (1.72 Mg ha−1). A sharp decrease of plant diversity (39.0%) inR. typhina12 years after restoration implied that soil C accumulation might not be sustainable in the long term. Our results suggest thatP. orientalisas an indigenous species adaptable to harsh environment may facilitate the succession of a diverse ecosystem and promote soil C accumulation in degraded lands of postmining areas.
Hepcidin and its therapeutic potential in neurodegenerative disorders
Authors: Qian Zhong‐Ming , Ke Ya 
Published: August 2019

AbstractAbnormally high brain iron, resulting from the disrupted expression or function of proteins involved in iron metabolism in the brain, is an initial cause of neuronal death in neuroferritinopathy and aceruloplasminemia, and also plays a causative role in at least some of the other neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, Huntington's disease, and Friedreich's ataxia. As such, iron is believed to be a novel target for pharmacological intervention in these disorders. Reducing iron toward normal levels or hampering the increases in iron associated with age in the brain is a promising therapeutic strategy for all iron‐related neurodegenerative disorders. Hepcidin is a crucial regulator of iron homeostasis in the brain. Recent studies have suggested that upregulating brain hepcidin levels can significantly reduce brain iron content through the regulation of iron transport protein expression in the blood‐brain barrier and in neurons and astrocytes. In this review, we focus on the discussion of the therapeutic potential of hepcidin in iron‐associated neurodegenerative diseases and also provide a systematic overview of recent research progress on how misregulated brain iron metabolism is involved in the development of multiple neurodegenerative disorders.
A fast and novel method for determining working volume in the reverberation chamber: Position of Tx antenna affect
Authors: Urul Bülent , Tütüncü Bilal , Helhel Selcuk 
Published: August 2019

AbstractReverberation chambers (RCs) are cheaper alternatives to anechoic chambers (ACs), and they are mainly made up of metal (ideally perfect and smooth) covered rooms. Their designs are not sophisticated as in AC, and EMC tests at precompliance level can be applied within a certain interval of error. For applying EMC tests to any equipment under test (EUT), the electric field uniformity in the room must be satisfied within a certain error. The volume having a uniform electric field is called as working volume (WV) where EUT for being subjected to EMC tests will be placed in. Positions and dimensions of scatterer such as Tx antenna that must be in the room determine the dimension of WV. Classically, position of transmitting antenna is changed continuously and randomly till obtaining WV and this process ends at any time within 3 days. Industry is always hurried and wants to know when to start testing. In this study, a fast and novel method with data mining has been proposed for overcoming this problem, and it achieves a success rate of 96% in 4‐5 hours. The model was tested for 10 different angles of rotation of the stirrer and 97% accuracy was achieved.
Multiple‐input‐multiple‐output/diversity antenna with dual band‐notched characteristics for ultra‐wideband applications
Authors: Kumar Sachin , Lee Gwan H. , Kim Dong H. , Mohyuddin Wahab , Choi Hyun C. , Kim Kang W. 
Published: August 2019

AbstractDesign and implementation of a four‐port ultra‐wideband (UWB) multiple‐input‐multiple‐output (MIMO)/diversity antenna with dual band‐notched characteristics are presented. The proposed diversity antenna is composed of four identical rhombic‐shaped monopole radiators arranged orthogonally to achieve better interelement isolation and polarization diversity. At each port, the MIMO antenna shows an impedance bandwidth (S11 ≤ –10 dB) of 10.5 GHz (2.8‐13.3 GHz) and an interelement isolation larger than 18 dB. Furthermore, in order to eliminate 3.5 GHz (Wi‐MAX) and 5.5 GHz (WLAN) bands from the UWB range, the antenna radiators are loaded with elliptical complementary split ring resonator structures. The MIMO performance metrics such as isolation, envelope correlation coefficient, and apparent diversity gain are calculated and presented. The MIMO/diversity antenna prototype is fabricated, and experimental results are verified with simulated results.
Sunflower shaped fractal filtenna for WLAN and ARN application
Authors: Hosain Md. Maqubool , Kumari Sumana , Tiwary Anjini Kumar 
Published: August 2019

AbstractThis manuscript proposes a triple band filtering antenna operating in the frequency range of 2.4 to 2.484 GHz, 4.2 to 4.5 GHz, and 5.15 to 5.825 GHz for possible application in WLAN and aeronautical radio navigation. Initially, a conventional square shaped monopole antenna is designed at 4 GHz, in which staircases are introduced at the edges to enhance bandwidth. Later, a triangle shape is carved out of square patch and cascaded at the junction to generate the proposed sunflower shaped fractal filtenna. Also, the ground plane is modified to further enlarge the bandwidth. By inserting C shaped complimentary split ring resonator slot on the feed line, a stop band is created at 3.5 GHz. Within the proposed frequency band of operation, the filtenna depicts omni‐directional radiation pattern, good selectivity, and low insertion loss. Also, the VSWR observed is less than 2 and peak antenna gain is 3.5 dBi. A good uniformity is achieved between the simulation and the experimental.