bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unconventional association between risk factors and knowledge of stroke among cerebrovascular disease survivors in China: A cross-sectional community-based study (FAST-RIGHT)
Authors: Li Shengde , Cui Li-Ying , Anderson Craig , Gao Chunpeng , Yu Chengdong , Shan Guangliang , Wang Longde , Peng Bin 
Published: June 2019

Abstract
Background: Cerebrovascular disease (CVD) survivors are at a risk of recurrent strokes, and early correct response to stroke is crucial to promote access to effective reperfusion therapy. This study aimed to investigate whether there is an association between increased risk factors and the intent to call emergency medical services (EMS) among CVD survivors.
Methods: A cross-sectional community-based study was conducted from January 2017 to May 2017, including 187 723 adults (age ≥ 40 years) across 69 administrative areas in China. A CVD survivor population of 6290 was analyzed. Multivariable logistic regression models were used to identify the association between the number of modifiable risk factors and stroke recognition and EMS calling, respectively.
Results: The estimated stroke recognition rate in CVD survivors with 0, 1-2, and 3-7 modifiable risk factors was 84.7% (321/379), 84.4% (2346/2780), and 85.6% (2673/3123), respectively. The rate of calling EMS in the 0, 1-2, and 3-7 modifiable risk factor groups was 66.0% (250/379), 62.7% (1744/2780), and 67.8% (2117/3123), respectively. The prevalence of cardiovascular diseases was higher among CVD survivors than the non-CVD population. The CVD survivors’ knowledge of recognizing stroke and intent to call EMS did not improve with an increasing number of modifiable risk factors, even after adjustment for multiple sociodemographic factors.
Conclusions: Despite being at a higher risk of recurrent stroke, Chinese CVD survivors showed poor knowledge of stroke, and their intent to call EMS did not improve with stroke risk. Special, targeted, and enhanced secondary stroke prevention education is needed for CVD survivors.
Barriers and facilitators of Pre-exposure Prophylaxis (PrEP) intention among Chinese Men who have sex with men
Authors: Su Xiaoyou , Si Mingyu , Yan Li , Jiang Yu , Liu Yuanli , Wei Chongyi , Yan Hongjing 
Published: June 2019

Abstract
Background

Despite strengthened efforts on HIV prevention and control, new HIV infections continue to increase among men who have sex with men (MSM) in China. Pre-exposure prophylaxis (PrEP), a highly effective HIV prevention tool, has recently been included in the China’s Action Plan of HIV Prevention and Control. To inform future PrEP implementation, this study is to identify barriers and facilitators of PrEP intention among MSM in China.

Methods

A cross-sectional survey was conducted among 300 MSM in Nanjing, Jiangsu Province in 2018. Demographic information, a 7-item high-risk behavior index, PrEP use intention, PrEP-related awareness and accessibility, and a 7-item public HIV stigma scale were included in the questionnaire. Bivariate and multivariate logistic regression analyses were conducted to identify factors associated with PrEP intention.

Results

Overall, just 55.7% of participants had ever heard of PrEP, while 75.3% expressed willingness to use PrEP if efficacy was assured. Beliefs that ‘PrEP can prevent HIV if taken as prescribed (aOR=4.84, p<0.001), and ‘PrEP can be scaled up in the community’(aOR=3.24, p<0.001), were positively associated with PrEP intention. Concerns of side effects were negatively associated with PrEP intention (aOR=0.32, p =0.006).

Conclusions

Given the relatively low awareness of PrEP-related information in China, interventions on awareness and knowledge targeting high risk individuals and community are needed to scale up of oral PrEP implementation once approved by China FDA.
Keywords MSM; HIV; PrEP; Intention
Effectiveness of Yi-Zhi-An-Shen Granules on cognition and sleep quality in older adults with amnestic mild cognitive impairment: protocol for a randomized, double-blind, placebo-controlled trial
Authors: Yue Shengnan , He Ting , Li Baiyang , Qu Yanqin , Peng Hongmei , Chen Jinxin , Lei Ming , Chen Chongli , Wu Wenbin 
Published: June 2019

Abstract
Background

Amnestic mild cognitive impairment (aMCI) is a syndrome characterized by significant forgetfulness that does not meet the criteria of dementia. Individuals with aMCI are at a bigger risk of progressing to dementia. Current studies suggest good sleep quality is linked with preserved cognition in the elderly, and sleep complaints are common among the elderly with amnesia. Therefore, improving their sleep may be helpful for maintaining and improving their cognitive capacity. According to the theory of traditional Chinese medicine, Yi-Zhi-An-Shen is an herbal compound which may ameliorate forgetfulness and sleep disorders. As growing evidence indicates that gut microbiome is associated with major mental symptoms, a hypothesis was proposed that Yi-Zhi-An-Shen Granules (YZASG) might work by alternating microbial abundance and diversity. In this study, the investigators intend to assess the efficacy of YZASG on global cognition in the elderly suffering from aMCI, and evaluate its safety as well as its potential mechanisms via sleep quality, fecal microbial 16SrDNA and metagenomics analyses, and serum markers.

Methods/design

This is a randomized, double-blind, placebo-controlled clinical trial. A total of 80 patients (aged 60~85 years) will be recruited and allocated randomly to a treatment group and a placebo group in a 1:1 ratio, then will be administered YZASG or isodose placebo three times a day. The intervention course is 16 weeks, with an 18-month follow-up. The primary outcome is Alzheimer Disease Assessment Scale-Cognitive subscale (ADAS-cog11). Secondary outcome measures are mini-mental state examination (MMSE), Montreal cognitive assessment (MoCA), Pittsburgh Sleep Quality Index (PSQI), serum concentrations of immunological factors and inflammatory cytokines, fecal microbiota. Fecal microbiota will only be collected at the baseline and endpoint of the intervention.

Discussion

The results of this trial will be conducive to assessing the safety and effectiveness on cognition of YZASG in intervening aMCI among the elderly and determining if it takes effect via the improvement of sleep quality, regulation of gut microbita, concentration of certain serum markers.
The efficacy and safety of Shenzhu Guanxin Recipe Granules for treatment of patients with coronary artery disease: protocol for a double-blind, randomized controlled trial
Authors: jin xiao , Wu Huanlin , Wu Bingxin , Li Yukai , Wang Xia , Liu Guoqing , Dang Xiaojing , Xu Danping 
Published: June 2019

Abstract
Background

Coronary artery disease (CAD) is one of the most common types of the cardiovascular diseases, previous pilot trials suggested that Traditional Chinese Medicine brought clinical benefits for patients with CAD. We will conduct this trial to determine the efficacy and safety of Shenzhu Guanxin Recipe Granules for treatment of patients with coronary artery disease.

Patients and Methods

This randomized controlled trial recruited 194 patients who were diagnosed CAD by clinical manifestations and examinations, in which coronary computed tomography angiography (CCTA) showed 50-70% of stenosis, with soft or mixed plaque. The included participants were randomly assigned to the case group and control group with a 1:1 allocation ratio; patients in the case group received SGR and usual care, and those in the control group received placebo (6 g/day for 6 months) and usual care. The endpoint of the study included calcium coverage score (CCS), C-Reactive protein (CRP), and the levels of blood lipids, tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-6 (IL-6), and ATP-binding membrane cassette transporter A1 (ABCA1) were calculated before recruiting at6th month. The indicators were Seattle Angina Questionnaire (SAQ) and TCM syndrome questionnaire scores at 0th, 3rd, and 6th months.

Discussion

This clinical trial may provide a reliable evidence regarding the clinical effectiveness and safety of SGR therapy for patients with CAD by clinical manifestations and examinations, in which coronary computed tomography angiography (CCTA) showed 50-70% of stenosis, with soft or mixed plaque.
Macular hole surgery recovery with and without face-down posturing: a meta-analysis of randomized controlled trials
Authors: Ye Ting , Yu Ji-guo , Liao Lin , Liu Lan , Xia Ting , Yang Lei-lei 
Published: June 2019

Abstract
Background: After pars plana vitrectomy with internal limiting membrane (ILM) peeling and gas tamponade, patients are often required to remain in a face-down position (FDP) to allow the gas bubble to push against the macular hole (MH) to promote hole closure. However, this position may be uncomfortable and inconvenient for the elderly and those with medical comorbidities; it may also lead to certain postoperative complications. Hence, this study aimed to evaluate and compare the effect of postoperative FDP and non-face-down position (nFDP) on the closure rate of MHs following MH surgery.
Methods: Randomized controlled trials (RCTs) were selected through an electronic search of the Cochrane Library, Pubmed, and Embase databases. Trial eligibility and risk of bias were assessed according to Cochrane review methods. The primary measures included overall MH closure rate and subgroup analysis based on MH size. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were estimated. Statistical analysis was performed using RevMan 5.0 software and Stata software 15.0.
Results: Five RCTs composed of a total of 183 eyes in the FDP group and 175 eyes in the nFDP group were included in this meta-analysis. Statistical meta-analysis revealed that the overall MH closure rate in the FDP group was significantly higher than that in the nFDP group (OR = 2.27, 95% CI: 1.02 to 5.05, P = 0.04). For MH sizes smaller than 400 µm, the subgroup meta-analysis indicated that the closure rate of the FDP group was not significantly higher than that of the nFDP group (OR = 1.32, 95% CI: 0.39 to 4.49, P = 0.66). However, when MH size was larger than 400 µm, there was a significantly higher closure rate in the FDP group (OR = 2.95, 95% CI: 1.10 to 7.94, P = 0.03).
Conclusions: Our results provide evidence that a face-down postoperative position seems to be unnecessary when MHs are smaller than 400 µm but may be highly recommended for MHs larger than 400 µm. Further RCTs with large sample sizes are warranted to validate these findings in future.
Phylogeny, Sequence-typing and virulence profile of uropathogenic E. coli (UPEC) strains from Pakistan
Authors: Ali Ihsan , Rafaque Zara , Ahmed Ibrar , Tariq Faiza , Graham Sarah E. , Salzman Elizabeth , Foxman Betsy , Dasti Javid Iqbal 
Published: June 2019

Abstract
Background Escherichia coli lineage ST131 predominates across various spectra of extra-intestinal infections, including urinary tract infection (UTI). The distinctive resistance profile, diverse armamentarium of virulence factors and rapid global dissemination of ST131 E. coli makes it an intriguing pathogen. However, not much is known about the prevalence and genetic attributes of ST131 lineage in Pakistan. Methods We estimated the prevalence and genetic attributes of E. coli ST131 isolates causing UTI among 155 randomly selected samples. Samples were analyzed by phylogenetic grouping, O-typing, fumC/fimH typing. Isolates were further tested for ESBL and virulence factors using PCR. Results Overall, 59% of the UPEC isolates belonged to the phylogenetic group B2, followed by D=28%, B1= 8% and A= 5%. Among 18 different Sequence-types, ST131 was the dominant lineage (n=71; 46%) out of which 72% of the isolates were assigned to phylogenetic group B2 while 61% adhered to serogroup O25b. FumC/fimH typing confirmed 49% of the ST131 as H30 sub-types. In this study, significant numbers of the identified ST131 isolates were MDR and 42% showed ESBL phenotypes, out of which 57% carried blaCTX-M-15. Moreover, different virulence factors were detected in following percentages: fimH,155 (100%), iutA 86 (55%), feoB 76 (49%), papC 75 (48%), papGII 70 (45%), kpsMTII 40 (26%), papEF 37 (24%), fyuA 37 (24%), usp 22 (14%), papA 20 (13%), sfa/foc 20 (13%), hlyA 18 (12%), afa 15 (10%), cdtB 11 (7%), papGI 6 (4%), papGIII 6 (4%), kpsMTIII 4 (3%) and bmaE2 (1%). Conclusion Conclusively, this study provides important insights into the genetic and virulence attributes of pandemic MDR ST131 strains involved in UTIs. It also highlights high prevalence of ST131-O25b-H30 UPEC isolates in local population, which was previously unreported from this part of globe.
Transcriptome changes reveal the genetic mechanisms of the reproductive plasticity of workers in lower termites
Authors: Ye Chenxu , Rasheed Humaira , Ran Yuehua , Yang Xiaojuan , Xing Lianxi , Su Xiaohong 
Published: June 2019

Abstract
Background: The reproductive plasticity of termite workers provides colonies with tremendous flexibility to respond to environmental changes, which is the basis for evolutionary and ecological success. Although it is known that all colony members share the same genetic background and that differences in castes are caused by differences in gene expression, the pattern of the specific expression of genes involved in the differentiation of workers into reproductives remains unclear. In this study, the transition of the female workers into neotenic reproductives (NRs) was induced by a groups of isolated workers (IWs) of Reticulitermes labralis, and then comparative transcriptomes were used for the first time to reveal the molecular mechanisms underlying the reproductive plasticity of workers.
Results: We identified 38,070 differentially expressed genes and found profile 5 to be the pattern of gene expression involved in the differentiation of the workers into reproductives. 12,543 genes were specifically upregulated in the IWs. Twenty-five signal transduction pathways classified into environmental information processing were related to the differentiation of workers into NRs. Ras functions as a signalling switch regulated the reproductive plasticity of workers.The catalase gene which is related to longevity was up-regulated in NRs.
Conclusion: We demonstrate that workers leaving the natal colony can induce the expression of stage-specific genes in the workers, which leads to the differentiation of workers into queens and suggests that the signal transduction along the Ras-MAPK pathway crucially controls the reproductive plasticity of the workers. This study also provides an important model for revealing the molecular mechanism of longevity changes.
Sistema diacrítico e transcendência em Merleau-Ponty
Authors: Camargo Jeovane 
Published: June 2019

A investigação do sentido diacrítico da linguagem leva a seentender a estrutura perceptiva como um arranjo opositivo não maisdependente exclusivamente das possibilidades do corpo. Esse ajuste
teórico da segunda fase do pensamento de Merleau-Ponty visa a su-perar os problemas da primeira fase. O percebido e a fala significam
agora por meio do arranjo de seus termos, não remetendo seu poderexpressivo a algum tipo de positividade ou “interioridade”.
Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells
Authors: Rubio Vicente , Iragavarapu Vijaya , Stawikowski Maciej J. 
Published: June 2019

Background:
Herein we report the multigram-scale synthesis, characterization and
application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in
biological applications. This fluorophore is devoid of rhodamine spirolactone formation and
furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and
quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA
towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of
αvβ3 integrins for in vitro assays.

Objective:
The main objective of this study is to efficiently prepare rhodamine B derivative,
devoid of spirolactone formation that would be suitable for bioconjugation and subsequent
bioimaging.

Methods:
Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using
N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine
dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding
sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was
used to image integrins on cancer cells.

Results:
The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total
yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent
spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized
by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of
87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and
straightforward. Imaging using standard filters on multimode plate reader and confocal microscope
was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF-
7 (breast cancer) cells were successfully imaged.

Conclusion:
We successfully derivatized rhodamine B to create an inexpensive, stable and
convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties
and it is suitable for bioconjugation and many imaging applications.
Significance of Asymptomatic Persistent Epstein-Barr Viral Load in
Pediatric Renal Transplant Recipients: North American Pediatric Renal
Trials and Collaborative Studies Report
Authors: Moudgil Asha , Martz Karen , Moore Therese , Harmon William E. , Dharnidharka Vikas R. 
Published: June 2019

Background:
Many pediatric transplant (TX) centers routinely monitor Epstein-Barr (EB) viral load (VL) by
real time quantitative PCR and intervene to prevent post-transplant lymphoproliferative disorder (PTLD). Some children
develop asymptomatic persistent VL (PVL). Outcome of different interventions in preventing PTLD and other undesired
effects on acute rejection (AR), graft failure (GF) and function amongst children with asymptomatic PVL is not known.

Methods:
NAPRTCS centers invited to enter data on children with asymptomatic PVL (≥ 6 months) into the EB VL
registry. Comparison group included children into the NAPRTCS TX arm during the same period without PVL or VL
monitoring. EB VL were arbitrarily divided into low (1-10), medium (>10-100) and high (>100times detection limit for
the center) ratio.

Results:
Of 645 children (18 centers), 85 (13.2%) developed onset of PVL at a mean of 6.4 ± 6.3 months post-TX. PVL
children were more likely to be younger (< 5 years) at TX and less likely to be African-American and majority (75.3%)
was mismatched for EBV (donor EBV IgG positive and recipient negative). Thymoglobulin induction was used in 29.4%
children with PVL versus 37% in controls (p=ns). PTLD developed in 7/85 (8.2%) children with PVL versus 5/560
(0.9%) controls (p < 0.0001). EB VL ratios were not different in those with and without PTLD. EB PVL as time varying
covariate did not affect patient survival, GF and AR (HR, 0.85, 0.53 and 0.99). The change in GFR overtime in children
with PVL was comparable to controls.

Conclusion:
Children with PVL (actual load not predictive) are at increased risk for PTLD, but not for AR, death, GF or
loss of graft function.
Efficacy of Extended Valganciclovir Prophylaxis in Preventing Cytomegalovirus Infection in Pediatric Kidney Transplantation
Authors: Faris Hashim , Jon A. Gregg , Vikas R. Dharnidharka 
Published: June 2019

Cytomegalovirus (CMV) is one of the most frequent opportunistic infection in renal transplant (RTx)
recipients. Valganciclovir (VGC) has been showed to be safe and highly effective in prophylaxis of CMV infection in
RTx recipients. Recently, an increase in delayed onset CMV disease has been noted with some arguing that longer
prophylaxis may decrease the late-onset disease.
We retrospectively tested the hypothesis that extended term prophylaxis (ETP) of VGC for 12 months is more effective
than short term prophylaxis (STP) of 6 months in preventing CMV infection and disease in pediatric RTx performed at the
University of Florida from July 2003 to December 2010. In this period, all recipients underwent prospective CMV PCR
(Polymerase Chain Reaction) monitoring and were maintained on similar immunosuppression.
Eighty six patients received RTx during that period. All eligible subjects had to have at least 12 months of graft survival and
18 months of follow up, leaving 73 eligible subjects in final study group. CMV infection or disease occurred in 6/29 (20%) in
the STP group vs 6/44 (14%) in the ETP group with no statistical significant difference (P= 0.42). Donor positive/recipients
negative CMV serology status (D+/R-) were associated with a higher risk of CMV infection in both univariate and
multivariate analysis (P=0.01). Anemia and Leucopenia directly associated with VGC were similar in both groups (P=0.58
and P=0.2 respectively). Biopsy-proven acute rejection was also non-significant in both groups (P=0.39).
Although ETP for CMV from 6 months to 12 months is safe and has minimal adverse effect, it did not reduce CMV
infection or disease. Further controlled studies in pediatrics age group are considered to compare longer versus shorter
periods of prophylaxis and their impact on prevention of CMV infection, resistance, cost, and toxicity.
Penangkal Sugesti Pickpocket di Kalangan Masyarakat Medan Deli
Authors: Sakdiah Halimahtun , Khuzaimah Khuzaimah , Suryadi Erwin , Santoso Kiki , Syavira Riri , Julani Rita 
Published: June 2019

Medan Deli adalah salah satu dari 21 Kecamatan di Kota Medan, Sumatera Utara, Indonesia. Kawasan Medan Deli terletak sekitar 100 PT lebih Kawan Industri seperti, Rumah Potong Hewan, Pajak Titipan, PT Swallow, Pengerajin Tas, Pengerajin Sofa, KIM, Wijaya Karya PT, Putri Hijau PT dan Industri Perabot Rumah Tangga. Pickpocket merupakan bentuk kejahatan mencopet dengan tipu daya sugesti dikenal dengan istilah bahasa Inggris yang dalam artian bahasa Indonesia adalah tukang copet atau pencopet. jenis pickpocket yaitu Medical, Forensic, Forensic. Pelatihan cara penangkal Sugesti Pickpocket akan dilaksanakan di Aula kantor Camat Medan Deli. Program penangkal Sugesti Pickpocket adalah program yang dirancang untuk Tenaga Kesejahteraan Sosial Kecamatan Medan Deli dan masyarakat sebagai wadah pelatihan mengantisipasi terjadinya kejahatan pencopetan dengan tipudaya sugesti dikalangan masyarakat. Kegiatan berlangsung selama4 bulan, meliputi sosialisasi pada bulan pertama, pelatihan kader pada bulan kedua, melakukan pengontrolan atau pengamatan kembali pada tempat sasaran pada bulan ketiga dan keempat. pelatihan berlangsung dimulai dari free test dan post test dapat mengetahui tingkat pengenalan masyarakat dan masyarakat yang pernah mengalami dari berbagai tipu daya sugesti. Beberapa masyarakat yang telah mengalami sugesti dimulai melalui secara langsung di dalam angkot dan melalui handphone mereka sangat mengalami trowma dengan adanya tindakan kejahatan yang mereka alami, sehingga tim pengabdian membuat pelatihan hynoterapi bagi masyarakat yang telah mengalami sugesti.