bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dosage and composition of bioactive glasses differentially regulate angiogenic and osteogenic response of human MSCs
Authors: Qazi Taimoor H. , Berkmann Julia C. , Schoon Janosch , Geißler Sven , Duda Georg N. , Boccaccini Aldo R. , Lippens Evi 
Published: October 2018

AbstractVascularization of the fracture site and cell‐mediated deposition of the mineralized matrix are crucial determinants for successful bone regeneration after injury. Ceramic biomaterials such as bioactive glasses (BAGs) that release bioactive ions have shown promising results in bone defect regeneration. However, it remains unclear how the dosage and composition of bioactive ions influence the angiogenic and osteogenic behavior of primary human mesenchymal stromal cells (MSCs). Here, we show that exposure to ionic dissolution products from 1393 and 45S5 BAGs can evoke distinct angiogenic and osteogenic responses from primary MSCs in a dose‐ and composition‐dependent manner. Significantly higher concentrations of the pro‐angiogenic factors VEGF, HGF, PIGF, angiopoietin, and angiogenin were detected in conditioned media (CM) from MSCs exposed to 45S5, but not 1393, BAGs. Application of this CM to human umbilical vein endothelial cells (HUVECs) resulted in robust 2D tube formation in vitro. Osteogenic differentiation of MSCs was assessed by gene expression analysis and mineralization assays. Low concentrations (0.1% w/v) of 1393 BAGs significantly enhanced the gene expression of RUNX2 and ALP and induced an earlier onset of matrix mineralization compared to all other groups. We further tested whether simultaneous exposure to both BAGs would improve both angiogenic secretion and osteogenic differentiation of MSCs, and did not find evidence to support this hypothesis. Our results provide evidence of BAG composition‐dependent enhancement of primary human MSCs' regenerative function, besides also underlining the importance of an in vitro evaluation of the dose–response relationship to translate BAG based approaches into safe and effective clinical therapies. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2827–2837, 2018., 2018
Preparation of photothermal palmitic acid/cholesterol liposomes
Authors: Linsley Chase S. , Zhu Max , Quach Viola Y. , Wu Benjamin M. 
Published: October 2018

AbstractIndocyanine green (ICG) is the only FDA‐approved near‐infrared dye and it is currently used clinically for diagnostic applications. However, there is significant interest in using ICG for triggered drug delivery applications and heat ablation therapy. Unfortunately, free ICG has a short half‐life in vivo and is rapidly cleared from circulation. Liposomes have been frequently used to improve ICG's stability and overall time of effectiveness in vivo, but they have limited stability due to the susceptibility of phospholipids to hydrolysis and oxidation. In this study, nonphospholipid liposomes were used to encapsulate ICG, and the resulting liposomes were characterized for size, encapsulation efficiency, stability, and photothermal response. Using the thin‐film hydration method, an ICG encapsulation efficiency of 54% was achieved, and the liposomes were stable for up to 12 weeks, with detectable levels of encapsulated ICG up to week 4. Additionally, ICG‐loaded liposomes were capable of rapidly producing a significant photothermal response upon exposure to near‐infrared light, and this photothermal response was able to induce changes in the mechanical properties of thermally responsive hydrogels. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1384–1392, 2019.
A novel patient‐specific three‐dimensional drug delivery construct for regenerative endodontics
Authors: Bottino Marco C. , Albuquerque Maria T. P. , Azabi Asma , Münchow Eliseu A. , Spolnik Kenneth J. , Nör Jacques E. , Edwards Paul C. 
Published: October 2018

AbstractEvoked bleeding (EB) clinical procedure, comprising a disinfection step followed by periapical tissue laceration to induce the ingrowth of undifferentiated stem cells from the periodontal ligament and alveolar bone, is currently the only regenerative‐based therapeutic approach to treating pulp tissue necrosis in undeveloped (immature) permanent teeth approved in the United States. Yet, the disinfection step using antibiotic‐based pastes leads to cytotoxic, warranting a biocompatible strategy to promote root canal disinfection with no or minimal side‐effects to maximize the regenerative outcomes. The purpose of this investigation was to develop a tubular three‐dimensional (3D) triple antibiotic‐eluting construct for intracanal drug delivery. Morphological (scanning electron microscopy), chemical (Fourier transform infrared spectroscopy), and mechanical (tensile testing) characteristics of the polydioxanone‐based triple antibiotic‐eluting fibers were assessed. The antimicrobial properties of the tubular 3D constructs were determined in vitro and in vivo using an infected (Actinomyces naeslundii) dentin tooth slice model and a canine method of periapical disease, respectively. The in vitro data indicated significant antimicrobial activity and the ability to eliminate bacterial biofilm inside dentinal tubules. In vivo histological findings demonstrated that, using the EB procedure, the tubular 3D triple antibiotic‐eluting construct allowed the formation of an appropriate environment that led to apex closure and the ingrowth of a thin layer of osteodentin‐like tissue into the root canal. Taken together, these findings indicate that our novel drug delivery construct is a promising biocompatible disinfection strategy for immature permanent teeth with necrotic pulps. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1576–1586, 2019.
Construction and comprehensive analysis of dysregulated long non‐coding RNA‐associated competing endogenous RNA network in clear cell renal cell carcinoma
Authors: Wang Jiawu , Zhang Chengyao , He Weiyang , Gou Xin 
Published: October 2018

AbstractObjectiveThis study aimed to assess the long noncoding RNA (lncRNA)‐microRNA (miRNA)‐messenger RNA (mRNA) regulatory network in clear cell renal cell carcinoma (ccRCC) by gene expression analyses.Materials and MethodsLncRNA, miRNA, and mRNA expression profiles in ccRCC were obtained from The Cancer Genome Atlas. Differentially expressed lncRNAs, mRNAs (cut‐off: |log 2 [fold change, FC])| > 2.0 and adjusted P < 0.01) and miRNAs (|log 2FC| > 1.5 and adjusted P < 0.01) were unveiled using R. Cox regression analysis was performed to identify prognostic factors of ccRCC related to overall survival (OS). A protein‐protein interaction (PPI) network was constructed for differentially expressed mRNAs (DEmRNAs) by Search Tool for the Retrieval of Interacting Genes (STRING). Key hub genes were screened from top 300 DEmRNAs. LncRNA‐miRNA and miRNA‐mRNA regulatory network were constructed and combined into the competing endogenous RNA regulatory network. Gene ontology biological terms were screened by STRING; Kyoto Encyclopedia of Genes and Genomes pathways were identified using the “clusterProfiler” package in R.ResultsA total of 2331, 1517, and 83 DEmRNAs, lncRNAs, and miRNAs were identified, respectively. Eleven lncRNAs (AC016773.1, HOTTIP, LINC00460, NALCN‐AS1, PVT1, TRIM36‐IT1, WT1‐AS, COL18A1‐AS1, LINC00443, LINC00472, and TCL6), three miRNAs (hsa‐mir‐21, hsa‐mir‐144, and hsa‐mir‐155), and three mRNAs (COL4A4, NOD2, and GOLGA8B) were associated with OS. Specifically, four lncRNAs (PVT1, LINC00472, TCL6, and WT1‐AS1) and one mRNA (Collagen Type IV Alpha 4 Chain) were verified as independent prognostic factors by Gene Expression Profiling Interactive Analysis. Eleven key hub genes were obtained by PPI analysis. “Cell adhesion molecules (CAMs),” “chemical carcinogenesis,” and “cytokine‐cytokine receptor interaction” were significantly enriched in the network.ConclusionThe findings clarify the pathogenesis of ccRCC and might provide potential therapeutic targets.
Sentinel Lymph Node Biopsy Versus Elective Neck Dissection for Stage I to II Oral Cavity Cancer
Authors: Cramer John D. , Sridharan Shaum , Ferris Robert L. , Duvvuri Umamaheswar , Samant Sandeep 
Published: October 2018

ObjectivesSentinel lymph node biopsy (SLNB) has been shown to be an accurate technique for staging the neck in early‐stage oral cavity squamous cell carcinoma (OCSCC) and has been incorporated in treatment guidelines as an option instead of elective neck dissection (END). However, utilization of SLNB in the United States remains unclear, and existing prospective studies did not directly compare survival between SLNB and END.MethodsWe conducted a retrospective cohort study of patients with stage I to II OCSCC (cT1‐2cN0cM0) who underwent staging of the neck in the National Cancer Data Base from 2012 to 2015. We compared the practice patterns and outcomes of patients who underwent SLNB versus END.ResultsWe identified 8,328 eligible patients with a median follow‐up of 35.4 months. SLNB was used for 240 patients, or 2.9% of stage I to II OCSCC. Completion neck dissection was avoided in 63.8% of patients undergoing SLNB. SLNB was associated with reduced perioperative morbidity, with median length of hospital stay of 1.0 days versus 3.0 days after END (P < 0.001). Perioperative 30‐day mortality was 0% after SLNB versus 0.7% after END (P = 0.42). Overall 3‐year survival was 82.0% after SLNB and 77.5% after END (P = 0.40). After adjustment, overall survival was equivalent between patients who underwent SLNB versus END (adjusted hazard ratio 1.03, confidence interval 0.67–1.59).ConclusionsSLNB for stage I to II OCSCC is associated with reduced length of hospital stay and equivalent overall survival compared with END. Despite these attributes, SLNB remains rarely used in the United States.Level of EvidenceNA Laryngoscope, 129:162–169, 2019
Tinnitus and taste disturbances reported after myringoplasty: Data from a national quality registry
Authors: Berglund Malin , Suneson Petter , Florentzson Rut , Fransson Mattias , Hultcrantz Malou , Westman Eva , Eriksson Per Olof 
Published: October 2018

Objectives/HypothesisPostoperative tinnitus and taste disturbances after myringoplasty are more common than previously reported.Study DesignThis study was a retrospective analysis of prospectively collected data from the Swedish National Quality Registry for Myringoplasty.MethodsThe analysis was performed on extracted data from all counties in Sweden collected from database A from 2002 to 2012 and database B from 2013 to 2016. Tinnitus and taste disturbance complications 1 year after myringoplasty were analyzed in relation to gender, age, procedure, and success rate. In database A, physicians reported tinnitus and taste disturbances. In database B, patients reported the complications.ResultsA major difference was found when the complications were reported by physicians compared to when the complications were reported by patients. In database A, tinnitus was reported in 1.2% of the patients and taste disturbances in 0.5%. In database B, the frequencies were 12.3% and 11.2%, respectively. Tinnitus and taste disturbances were more frequent after conventional myringoplasty compared to those after fat grafting and were more frequent after primary compared to those after revision surgery when reported by physicians. Patients, however, reported the same frequency of tinnitus after fat graft myringoplasty compared to that after conventional myringoplasty (12.0% vs. 12.6%) and fewer taste disturbances after revision surgery. In follow‐up assessments, complications persisted after surgery over a long time period.ConclusionTinnitus and taste disturbances are more common after myringoplasty when patients report their symptoms than when physicians report the symptoms.Level of Evidence2b Laryngoscope, 129:209–215, 2019
Percutaneous versus surgical tracheostomy: timing, outcomes, and charges
Authors: Yang Anthony , Gray Mingyang L. , McKee Sean , Kidwai Sarah M. , Doucette John , Sobotka Stanislaw , Yao Mike , Iloreta Alfred 
Published: October 2018

Objectives/HypothesisThe purpose of this study was to compare timing of procedure, patient characteristics, outcomes, and charges for patients who underwent percutaneous versus surgical tracheostomy.Study DesignRetrospective cohort study.MethodsA retrospective analysis was performed for all patients who underwent tracheostomy in 2015 to 2016 in New York State. Patients were identified using International Classification of Diseases, 10th Revision, Clinical Modification codes and stratified to the type of tracheostomy performed. The primary outcome of interest was mortality at index stay. Secondary outcomes of interest included length of stay and total hospitalization charges.ResultsOf the 8,682 patients, 2,488 (28.7%) underwent percutaneous and 6,194 (71.3%) underwent surgical tracheostomy. At hospitals where both procedures were performed, percutaneous tracheostomy patients were older, had more comorbidities, and had lower income (P < .05). Timing of the tracheostomy relative to admission did not affect the type of tracheostomy performed. While controlling for patient characteristics and complications during the visit, percutaneous tracheostomy was associated with increased mortality (odds ratio [OR]: 1.17, 95% confidence interval [CI]: 1.03‐1.33, P = .0153) and increased hospital charges (OR: + 7.76%, 95% CI: 5.4‐10.11, P < .0001). Length of stay was not affected by procedure type.ConclusionsSurgical tracheostomies are more commonly performed than percutaneous tracheostomies across New York State. Older, lower‐income, and sicker patients have a higher chance of receiving percutaneous tracheostomies. Percutaneous approaches were associated with statistically significant increased mortality and higher charges despite no difference in length of stay. Further studies are needed to determine if these differences in outcomes are clinically significant.Level of EvidenceNA Laryngoscope, 128:2844–2851, 2018
Risk factors for thyroid surgery–related unilateral vocal fold paralysis
Authors: Chen Hung‐Chun , Pei Yu‐Cheng , Fang Tuan‐Jen 
Published: October 2018

Objectives/HypothesisWe aimed to identify the risk factors for iatrogenic unilateral vocal fold paralysis (UVFP) caused by thyroid surgery, to allow the identification of patients requiring nerve‐protection procedures and monitoring technologies.Study DesignRetrospective case study in a medical center.MethodsPatients who underwent thyroid surgery from April 2011 to February 2016 and who were diagnosed with UVFP by laryngoscopy and laryngeal electromyography were included. Patient demographics, types of surgery, and characteristics of the thyroid lesions were analyzed.ResultsSixty (2.1%) of 2,815 patients who received thyroid surgery developed UVFP. The risk of UVFP was higher in patients over 60 years old (odds ratio, 1.89; 95% confidence interval, 1.01‐3.26; P = .01). Involvement of the external branch of superior laryngeal nerve (EBSLN) occurred in 19 (31.7%) of the 60 UVFP patients, and was more likely to occurr in patients with diabetes mellitus (odds ratio, 14.19; 95% confidence interval, 3.80‐52.94; P < .001). The incidence of UVFP and involvement of the EBSLN differed among surgery types, and was the highest among patients undergoing total thyroidectomy with neck dissection (TTND) (10/158, 6.3% and 5/158, 3.2%, respectively).ConclusionsThe risk of thyroid surgery–related UVFP is higher in older patients. EBSLN involvement is more likely in patients with diabetes mellitus. TTND is associated with higher risks of UVFP and EBSLN injury than other types of surgery, implying the need of intraoperative nerve monitoring in these high‐risk characteristics.Level of Evidence4 Laryngoscope, 129:275–283, 2019
Increasing wind erosion resistance of aeolian sandy soil by microbially induced calcium carbonate precipitation
Authors: Tian Kanliang , Wu Yuyao , Zhang Huili , Li Duo , Nie Kangyi , Zhang Shican 
Published: October 2018

AbstractWind erosion of aeolian sandy soil can cause serious land degradation and other environmental problems, and thus its prevention and control is very important. However, there has not been a very effective way to prevent this severe wind erosion until now, and traditional measures such as mechanical methods, chemical sand‐fixing methods, and agronomic methods also have many disadvantages. The main objective of this study is to evaluate feasibility of microbially induced calcite precipitation (MICP) as a novel soil‐strengthening technique, to cement aeolian sandy soil, and reduce wind erosion risk. For this purpose, aeolian sandy soil was cemented withSporosarcina pasteuriithrough MICP technique, and the physical and mechanical properties and wind erosion resistance of cemented aeolian sandy soil were tested. The results show that wind erosion resistance of aeolian sandy soil can be effectively improved by sprayingS. pasteuriisolution and cementing solution (equal concentration of urea and calcium chloride mixture) into it from the surface, and the cemented aeolian sandy soil had good wind erosion resistance. Finally, morphology of precipitated CaCO3crystals was studied using scanning electron microscope, optical microscope, and X‐ray diffraction. The calcium carbonate crystals produced in aeolian sandy soil were calcite, and the calcium carbonate crystals had polyhedral, spherical, or flower clusters crystal morphology. The results of this study demonstrate an effective way to use MICP technology to cement aeolian sandy soil and prevent wind erosion, and provide a new way for wind erosion prevention.
Difference in wind erosion characteristics between loamy and sandy farmlands and the implications for soil dust emission potential
Authors: Wang Rende , Zhou Na , Li Qing , Chang Chunping , Guo Zhongling , Li Jifeng 
Published: October 2018

AbstractDust emission depends directly and indirectly on soil particle distribution, but little information is available that examines dust emission potential and its relationship with wind erosion for different soils. This study aimed to elucidate the difference in wind erosion characteristics between loamy farmland (LF) and sandy farmland (SF) and discuss the influence of this difference on dust emission potential. Field observations were conducted during 2012–2016 in Bashang district, North China. With increasing friction wind speed, the horizontal mass flux increased exponentially in LF but obeyed the power function in SF. The horizontal mass flux was higher in SF than in LF, but the gap narrowed from 766 times at friction wind speed of 0.3 m s−1 to 50 times at 0.6 m s−1. The dry aggregate size distribution of sediment in LF was finer than in SF. Fine particle content (diameter < 0.05 mm) in windblown sediment was much higher in LF (31.39%) than in SF (5.92%), which was influenced by parent soils. With height increasing, the mass percentage of sediment decreased more rapidly in SF than in LF, with 86% and 66% of sediment clustering at height 0–20 cm, respectively. Because the horizontal mass flux and dust content in sediment both increased more quickly in LF than in SF with increasing friction wind speed, the dust emission potential was larger in LF than in SF under strong winds. The adverse effect of dust emission in loamy soils on atmospheric pollution should receive more attention in strong wind erosion events.
Endoscopic training—is the future three‐dimensional?
Authors: Nassimizadeh Abdul , Zaidi S.M. , Nassimizadeh Mohammad , Kholief Amir , Ahmed Shahzada K. 
Published: October 2018

Background/ObjectivesEndoscopic surgery has a distinct disadvantage compared to direct vision: loss of binocular vision. Three‐dimensional endoscopy has been welcomed due to the promise of improving stereopsis.MethodsProspective randomized study of junior doctors with minimal endoscopic experience, using both two‐dimensional and three‐dimensional, zero‐degree, 4‐mm Storz endoscopes. Data was collected using validated, standardized training models, both objectively and subjectively. Paired comparisons between variables relating to the endoscopes were performed using Wilcoxon's tests. Operators were then split into groups based on their endoscope preference, with comparisons made using Mann‐Whitney tests for Likert scale responses, Kendall's tau for ordinal variables, and Fisher's exact tests for nominal variables.ResultsReduction of field of vision of three‐dimensional endoscopy by 2%. Significant findings included decreased past‐pointing, improved depth and perception and image quality.ConclusionThe use of an endoscopic endonasal approach with three‐dimensional technology has measurable advantages for novice users, and highlights potential tailoring of future surgical trainingLevel of Evidence1b
Integration of aerial infrared thermography and in situ radon‐222 to investigate submarine groundwater discharge to Pearl Harbor, Hawaii, USA
Authors: Kelly Jacque L. , Dulai Henrietta , Glenn Craig R. , Lucey Paul G. 
Published: October 2018

AbstractThe largest freshwater spring complex in the Hawaiian Islands discharges groundwater into Pearl Harbor, Oahu, which is the largest estuary in the islands. This discharge occurs as subaerial and submarine springs, as non‐point‐source (diffuse) seepage, and as baseflow to streams. We conducted airborne thermal infrared remote sensing surveys of sea surface temperatures and ocean‐based 222Rn surveys of surface and deep waters to evaluate the efficacy of combining these methods to locate and quantify these differing groundwater discharges to the harbor. High spatial‐resolution (≤ 3.2 m) sea surface temperature data were advantageous for identifying and differentiating point‐source and diffuse groundwater discharge. Surveys of surface waters for 222Rn confirmed point‐source and diffuse seepage zones identified from the sea surface temperature maps. The 222Rn mid‐ and deep‐water surveys were crucial for locating benthic seepage not detectable by the remote sensing method. Groundwater discharge estimates from radon time‐series data agreed with radon surface water survey estimates. Fresh groundwater discharge estimates were also of the same order of magnitude as previous hydrologic estimates of spring discharge. We produced linearly and highly correlated relationships between plume areas and groundwater discharge by combining the thermal infrared and radon techniques. Such relationships may provide a way to up‐scale local groundwater discharge assessments to a regional area.
Phosphazene Bases as Organocatalysts for Ring‐Opening Polymerization of Cyclic Esters
Authors: Liu Shaofeng , Ren Chuanli , Zhao Na , Shen Yong , Li Zhibo 
Published: October 2018

AbstractOver the past several years, organocatalyzed polymerization reactions have attracted considerable attention, and these efforts have led to major advances. A large number of organic compounds have been proven active for the polymerization of a large variety of monomers. In particular, phosphazene bases (PBs) are a family of extremely strong, non‐nucleophilic, and uncharged auxiliary bases, and have shown their remarkable potential as organocatalysts for the ring‐opening polymerization (ROP) of cyclic monomers. By deprotonation of weak acids or in combination with lithium cation, PBs significantly enhance the nucleophilicity of the initiator/chain‐end, thus allowing fast and usually controlled anionic polymerization. In this feature article, the recent advances in phosphazene‐catalyzed ROP of cyclic esters are summarized. This review is divided into three sections, including general features, design and synthesis, and catalytic applications. It aims to provide a critical analysis of PB‐mediated ROP systems and a useful guide for the further design of organocatalysts applied to polymer synthesis. An outlook is given at the end.