bluuebunny/crossref_metadata_2025_split huggingface dataset:
Case report: Pediatric intraventricular Rosai-Dorfman disease: clinical insights and surgical strategies in a decade-long observational study and literature review
Authors: Liu Dayuan , Li Ning , Zhu Yubo , Zhong Yunxiang , Deng Guolong , Wang Mingfa , Zhang Caicai , Feng Jigao 
Published: November 2024

BackgroundRosai-Dorfman disease (RDD), or sinus histiocytosis with massive lymphadenopathy (SHML), is a rare benign disorder characterized by the proliferation of histiocytes of uncertain origin. Central nervous system (CNS) involvement, particularly intraventricular, is exceptionally rare and poses significant diagnostic challenges due to its non-specific clinical and radiographic presentation. This study aims to present a case of intraventricular RDD and review existing literature on its clinical features, treatment strategies, and prognosis.MethodsWe report the case of a five-year-old male with recurrent headaches and epilepsy caused by an intraventricular mass. The mass was surgically resected and histopathological examination was performed to confirm the diagnosis. A comprehensive literature review was conducted to identify similar cases of intraventricular RDD, focusing on clinical features, diagnostic methods, treatment strategies, and outcomes.ResultsHistopathological examination of the resected tumor revealed typical features of RDD, including large histiocytes, lymphocyte infiltration, and immunohistochemical positivity for CD68, S-100, and Vimentin. The patient remained asymptomatic ten years post-surgery with no recurrence of epilepsy or tumor. The literature review identified six similar cases, all of which showed favorable outcomes post-surgery, highlighting the self-limiting nature and favorable prognosis of intraventricular RDD following surgical resection.ConclusionIntraventricular RDD, though rare, should be considered in the differential diagnosis of intraventricular masses in pediatric patients. Surgical resection remains the primary treatment modality, and histopathological confirmation is essential for accurate diagnosis. The prognosis is generally favorable with appropriate surgical intervention, although recurrence can occur, necessitating long-term follow-up. Further research is required to refine diagnostic criteria and explore adjuvant therapies for improved management of this rare CNS disorder.
The role of ATP6V0D2 in breast cancer: associations with prognosis, immune characteristics, and TNBC progression
Authors: Zhang Jingyu , Yang Lixian , Xu Bin , Ji Haibo , Liu Shuo , Wang Xiaohan , Li Xiaolong , Wang Quanle , Song Zhenchuan 
Published: November 2024

ObjectiveResearches have identified ATPase H+ transporting V0 subunit d2 (ATP6V0D2) as a significant factor in various cancers. However, its prognostic value in breast cancer (BRCA) and its biological role in BRCA cells remain unclear.MethodsIn this research, we examined the varying expression levels of ATP6V0D2 in both BRCA and normal breast tissue by utilizing information derived from databases including the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO), along with clinical samples. Survival studies were carried out to investigate the link between ATP6V0D2 levels and prognosis in BRCA patients. A series of enrichment analyses identified possible pathways associated with the differentially expressed genes in BRCA. The relationships among ATP6V0D2 expression, immune characteristics, and gene mutation were evaluated using Spearman’s test. Finally, the expression of ATP6V0D2 was identified by quantitative real-time polymerase chain reaction (RT-qPCR) alongside western blot analysis. Additionally, Cell Counting kit-8 (CCK-8), Colony formation, Transwell, Scratch healing, and Mouse xenograft tumor assays were conducted to assessed the impact of ATP6V0D2 knockdown on the biological functions in TNBC.ResultsATP6V0D2 exhibited high expression in a range of cancers and correlated with unfavorable prognosis in BRCA. Functional enrichment analyses revealed enrichment of extracellular matrix-receptor interaction, focal adhesion, and the signaling pathway of tumor growth factor-beta in the high ATP6V0D2 expression group. Additionally, ATP6V0D2 was closely associated with immune checkpoints. Its expression positively associated with the infiltration levels of macrophage and neutrophil, but inversely with CD8+ T and plasmacytoid dendritic cells. Mutation analysis revealed that PIK3CA, linked to decreased OS, exhibited a higher mutation rate in the ATP6V0D2 high expression group. Furthermore, ATP6V0D2 knockdown inhibited TNBC cells invasion, migration, and proliferation abilities.ConclusionATP6V0D2 acts as a promising indicator for both diagnosis and prediction of outcomes in breast cancer and could potentially be a novel therapeutic target for BRCA.
Chen’s peiyuan tang and premature ovarian failure: unveiling the mechanisms through network pharmacology
Authors: Yang Xiao , Mao Yi-Ming , Yao Chong , Song Ding-ming , He Yi-bo , Shen Wei 
Published: November 2024

BackgroundChen’s Peiyuan Tang (CSPYT) is a compound herbal formula that has shown the potential to enhance ovarian function and reduce autophagy in ovarian granulosa cells, which plays a crucial role in follicular development and maturation. The application of Chinese herbal medicine offers a promising alternative to traditional hormone replacement therapy (HRT).MethodsThis study explores CSPYT’s therapeutic mechanisms in treating POF, focusing on its modulation of autophagy through network pharmacology and transcriptomics-based analysis, predicting potential interactions and pathways. KGN cell line and rat ovarian granulosa cells were used for in vitro experiment. 4-Hydroperoxy cyclophosphamide(4-HC) stimulation was carried out for establishing the POF cell model. Q-PCR, Western Blot, Transmission electron microscopy to detect the results.ResultsAccording to the drug and disease database, the common targets of Chen’s Peiyuan Tang and premature ovarian failure were screened, combined with autophagy gene targets and transcriptome analysis, and finally 8 intersection targets were obtained, namely CDKN1B, MAPK3, PRKCD, CDKN1A, MAPK1, RAF1, BIRC5, CTSB. Enrichment analysis of 8 genes found that they were closely related to the animal autophagy pathway. Construct PPI network diagram. CytoScape 3.9.1 builds CSPYT Drug Target-POF Disease Target-Autophagy Gene Network Diagram. Based on the PPI network diagram and CytoScape 3.9.1 analysis results, it is estimated that MAPK1 and MAPK3 are the key targets of CSPYT in the treatment of POF. The eight final intersection targets were docked with the corresponding active pharmaceutical ingredients. The one that docked most closely with the MAPK family was naringenin. In cell experiment verification, it was confirmed that Chen’s Peiyuan Tang can inhibit the MAPK signaling pathway, significantly reduce the number of autophagosomes, and reduce autophagy damage in ovarian granulosa cells.DiscussionCSPYT can inhibit the MAPK signaling pathway, prevent autophagy overexpression and restore ovarian granulosa cell function, effectively alleviating the disease pressure of POF.
Overexpression of endothelial S1pr2 promotes blood–brain barrier disruption via JNK/c-Jun/MMP-9 pathway after traumatic brain injury in both in vivo and in vitro models
Authors: Cheng Hongbo , Men Yijiao , An Yaqing , Yu Jiegang , Zhang Gengshen , Li Jiaming , Wang Xiaoliang , Sun Guozhu , Wu Yang 
Published: November 2024

ObjectivesThe disruption of blood-brain barrier (BBB) is associated with poor outcomes of TBI patients. Sphingosine-1-phosphate receptor 2 (S1pr2), a member of the G protein-coupled receptor family, is involved in endothelial activation and the regulation of vascular integrity. We hypothesized that the inhibition of S1pr2 may alleviate BBB disruption and explored potential underlying molecular mechanisms.MethodsLesion volumes were assessed utilizing Nissl staining; neurological outcomes were evaluated through a battery of neurobehavioral assessments; phenotype-associated proteins were scrutinized via Western blot analysis; levels of reactive oxygen species (ROS), neuronal apoptosis, and S1pr2 expression were determined using immunofluorescence staining. The impact of S1pr2 inhibition after TBI and its underlying mechanism were elucidated using the selective S1pr2 inhibitor JTE-013, the JNK phosphorylation inhibitor SP600125, and cellular models. Chip-qPCR was employed to further elucidate the binding sites of the transcription factor c-Jun.ResultsThe expression of S1pr2 significantly increased following TBI in mice. Pharmacological inhibition of S1pr2 alleviated secondary injury with reduced lesion volume, ROS generation, cerebral oedema, neurological deficits, and neuronal apoptosis; BBB disruption was also mitigated, accompanied by reduced degradation of tight junction proteins and decreased induction of matrix metalloproteinases-9 (MMP-9) post-TBI. Mechanistically, TBI induces an increase in S1pr2 specifically in endothelial cells, leading to the promotion of MMP-9 transactivation by enhancing JNK/c-Jun signaling. This results in the degradation of tight junction proteins and increased BBB permeability. Through in vitro and in vivo Chip-qPCR experiments, we verified that AP-1a and AP-1b of MMP-9 promoter function as binding sites for phosphorylated c-Jun.ConclusionOur findings identify a previously undisclosed role of S1pr2 in the pathophysiology of TBI. The S1pr2 inhibition presents a novel approach to alleviate BBB disruption after TBI through regulating the JNK/c-Jun/MMP-9 pathway.
O304 alleviates abdominal aortic aneurysm formation via AMPK/mTOR/MMP pathway activation
Authors: Sun Daohan , Du Yaming 
Published: November 2024

BackgroundAbdominal aortic aneurysm (AAA) rupture is a significant cause of mortality in the elderly population. Despite experimental models identifying promising pharmacological therapies, there is still a lack of pharmacological interventions for AAA prior to surgery. This study aims to evaluate the regulatory role of the novel adenosine monophosphate-activated protein kinase (AMPK) agonist O304 in AAA formation and explore its underlying molecular mechanisms.MethodsWe evaluated the expression of AMPK signaling pathway components and contractile vascular smooth muscle cell (VSMC)-related genes in AAA samples from mice using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We evaluate the TGF-β expression by western blotting and RT-qPCR and TGF-β concentration in blood by ELISA. We developed an in vitro model of transforming growth factor-β (TGF-β)-induced VSMC phenotypic switching. After treatment with O304, we analyzed the expression of contractile genes and proteins in VSMCs by immunofluorescence and Western blotting. We also evaluated the expression of AMPK signaling pathway components and matrix metalloproteinases by western blotting and immunofluorescence analysis. We established a mouse model of AAA to evaluate the impact of O304 on aneurysm diameter and blood pressure, analyzed VSMC phenotypic switching through immunofluorescence analysis, and assessed the regulatory effects of O304 on AMPK signaling in the mouse model of AAA by Western blotting.ResultsAMPK signaling pathway components and contractile genes in VSMCs were downregulated in mouse AAA samples, underscoring the crucial role of AMPK signaling in VSMC phenotypic switching. In the TGF-β-induced model of VSMC phenotypic switching, O304 activated AMPK signaling and prevented VSMC phenotypic switching from the contractile to the synthetic phenotype. Moreover, O304 significantly activated AMPK signaling, increased the proportion of contractile VSMCs, and reduced AAA formation and blood pressure in the mouse model of AAA.ConclusionDuring AAA development, VSMCs transitioned from the contractile to the proliferative phenotype, a process that has previously been associated with AMPK pathway inhibition. O304, an AMPK agonist, activated the AMPK pathway, preventing VSMC phenotypic switching and inhibiting AAA formation. These findings highlight the therapeutic potential of targeting the AMPK pathway in AAA.
Did social isolation affect anxiety and sleep quality of elite soccer players during the COVID-19 lockdown? Comparisons to training before distancing in the pandemic and outlook for mental health
Authors: da Cruz Whyllerton Mayron , Coimbra Danilo Reis , Vilarino Guilherme Torres , dos Santos Amândio Manuel Cupido , da Silva Vernon Furtado , Mancone Stefania , Falese Lavinia , Diotaiuti Pierluigi , Andrade Alexandro 
Published: November 2024

ObjectiveTo analyze the anxiety levels and sleep quality of elite soccer athletes in training pre-pandemic and during the lockdown caused by COVID-19.MethodThis is an exploratory study with a longitudinal design carried out with elite soccer athletes from two Brazilian soccer clubs. Data collection took place in person pre-pandemic (training) and online (during lockdown) between February and May 2020. The instruments used to assess sleep, daytime sleepiness, and anxiety were the Pittsburgh Sleep Quality Index (PSQI), Epworth Daytime Sleepiness Scale-(ESS-BR), and Competitive Anxiety Scale (SCAT). For data analysis, descriptive statistics (frequencies, percentages, maximum and minimum) and non-parametric inferential statistics were used, establishing a significance of p &lt; 0.05.ResultsIn total, 76 male soccer athletes participated in the study. A significant increase was observed in anxiety levels in confinement compared to pre-pandemic training (p = 0.017; g = 0.83), and sleepiness significantly reduced in training compared to baseline levels (p = 0.007; g = 0.48). The athletes demonstrated good sleep quality and the pandemic did not significantly alter daytime sleepiness compared to training and baseline.ConclusionFrom the results it can be concluded that elite soccer athletes presented alterations in anxiety levels compared to training during confinement, however, no effects of confinement were observed on sleep quality and sleepiness. New studies are needed to analyze the long-term consequences of the pandemic and the relationships between anxiety and sleep in training and competition in athletes.
Barriers impeding research data sharing on chronic disease prevention among the older adults in low-and middle-income countries: a systematic review
Authors: Mosha Neema Florence Vincent , Ngulube Patrick 
Published: November 2024

IntroductionChronic diseases, including cardiovascular disease, diabetes, cancer, and chronic respiratory diseases, are a growing public health concern in low-and middle-income countries (LMICs) among the older population. The current review aimed to identify the main barriers that impede researchers from sharing research data on the prevention of chronic diseases in older adults living in LMICs). The review included both older women and men from these countries.MethodsStudies were selected from 11 databases, including Web of Science, Scopus, PubMed, Taylor and Francis, Biomedical Central, BioOne, CINAHL, EBSCOHost, ScienceDirect, Wiley Online, and Google Scholar, were then transferred to CADIMA, an online tool for screening purposes, and a total of 1,305,316 studies were identified through a robust search strategy. CADIMA also ensured the quality of all studies in this review. The sampling techniques were performed by selecting and screening studies per this review’s eligibility criteria. Ultimately, 13 studies were found to meet these criteria. A PRISMA flow chart was used to map out the number of studies that were identified, included, and excluded.ResultsFive main barriers were consistently highlighted, including a lack of necessary resources (9, 69%), dealing with complex and sensitive research data (2,15%), lack of policies, procedures, guidelines (5,38%), medical big data processing and integration (2,15%), and inadequate ethical considerations, legal compliance, and privacy protection (6,46%). Discussion: By shedding light on these obstacles, researchers can develop strategies to overcome the identified barriers and address areas requiring further investigation. The registration details of this review can be found under PROSPERO 2023 CRD42023437385, underscoring the importance of this review in advancing our collective understanding of chronic disease prevention among older adults worldwide.Systematic review registrationPROSPERO, identifier CRD42023437385, available at: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023437385.
Human papillomavirus self-sampling versus provider-sampling in low- and middle-income countries: a scoping review of accuracy, acceptability, cost, uptake, and equity
Authors: Otieno Jenifer Akoth , Were Lisa , Nyanchoka Moriasi , Olwanda Easter , Mulaku Mercy , Sem Xiaohui , Kohli Mikashmi , Markby Jessica , Muriuki Angela , Ochodo Eleanor 
Published: November 2024

IntroductionHPV self-sampling is a relatively new, cost-effective and widely accepted method, however, uptake in LMICs remains limited. We aimed to map out the evidence and identify gaps in accuracy, acceptability, cost, equity and uptake of self-sampling vs. provider-sampling in LMICs.MethodsWe searched: MEDLINE, EMBASE, CINAHL, SCOPUS, Web of Science, and Global Index Medicus, from 1946 to July 2023. Inclusion criteria entailed studies focusing on self-sampling alone or compared to provider-sampling for HPV testing and reporting on at least one outcome of interest (accuracy, acceptability, cost, equity, or uptake). Two authors independently screened titles, abstracts, and full texts, resolving disagreements through discussion. Data was extracted by one reviewer independently, with quality checks by senior authors, and results were synthesised narratively.ResultsOur search yielded 3,739 records, with 124 studies conducted on 164,165 women aged 15–88 years between 2000 and 2023 included. Most studies were from the African region (n = 61, 49.2%). Designs included cross-sectional (n = 90, 81.1%), randomised (n = 5, 4.5%), modelling (n = 4, 3.6%), micro-costing (n = 2, 1.8%), and non-randomised crossover (n = 1, 0.9%) studies. Outcomes included; acceptability (n = 79, 63.7%), accuracy (n = 51, 41.1%), cost (n = 7, 5.6%), and uptake (n = 7, 5.6%). Most studies reported that participants preferred self-sampling, with only a few studies (n = 7, 8.9%) studies favouring provider-sampling. The sensitivity and specificity of self-sampling ranged from 37.5–96.8% and 41.6–100.0%, respectively. One study directly compared the sensitivity and specificity of dry self-collected vs. wet provider-collected sample transportation. Laboratory costs were similar, but overall costs were lower for self-sampling. Uptake was higher for self-sampling in five of the seven studies. Most studies (n = 106) mentioned equity factors like age (n = 69, 65.1%), education (n = 68, 64.2%) and place of residence (n = 59, 55.6%) but no analysis of their impact was provided.ConclusionHPV self-sampling is acceptable and cost-effective but, evidence of its accuracy shows varying sensitivity and specificity. Evidence on the accuracy of dry self-collected vs. wet provider-collected sample transportation is limited. Research evaluating HPV self-sampling’s accuracy, including comparisons of transportation modes, uptake, the impact of equity factors in LMICs and comparisons with high-income countries is essential to inform cervical cancer screening uptake.Systematic review registrationhttps://doi.org/10.17605/OSF.IO/34TUY.
Rural–urban differences in the association between home-based community care services satisfaction and mental health status among older adults in Zhejiang Province, China: a cross-sectional study
Authors: Ying Yuchen , Dong Lifang , Zhang Li , Kong Fanqian , Yang Jiani , Huang Xiaoling 
Published: November 2024

ObjectiveThis study aims to examine the association between home-based community care services (HBCCS) and mental health in older adults and specifically analyzes rural–urban differences in the association.MethodsThis cross-sectional study enrolled 852 older adults from Zhejiang Province, China. The Patient Health Questionnaire (PHQ-9), 7-item Generalized Anxiety Disorder (GAD-7), University of California Los Angeles 3-item Loneliness Scale (UCLA-3), and the Mental Health (MH) component score of the 36-item Short Form (SF-36) were used to measure self-reported mental health status. Four categories of community care services were examined: daily, medical, social and recreational, and spiritual comfort. Satisfaction with community care services was assessed using self-reported measures. We used a multiple linear regression model.ResultsSatisfaction with daily care services, social and recreational services, and spiritual comfort services in rural older adults was significantly higher than in urban older adults (p = 0.016, p &lt; 0.001, p &lt; 0.001, respectively). Rural older adults reported lower scores on the PHQ-9, GAD-7, and UCLA-3 than urban older adults (p &lt; 0.001, p = 0.003, p = 0.001, respectively) and had significantly higher scores on the SF-36 MH than urban older adults (p &lt; 0.001). Among urban older adults, medical care services satisfaction was negatively related to the PHQ-9 and UCLA-3 scores (β = −0.296, p = 0.004; β = −0.447, p = 0.009, respectively). A lower UCLA-3 score was associated with higher levels of satisfaction with social and recreational services and with spiritual comfort services (β = −0.426, p = 0.010; β = −0.523, p = 0.002, respectively). A higher level of spiritual comfort services satisfaction was associated with a lower SF-36 MH score (β = 0.646, p &lt; 0.001). Among rural older adults, medical care services satisfaction was negatively related to the GAD-7 score (β = −0.327, p = 0.028).ConclusionHome-based community care services satisfaction was positively associated with older adults’ mental health status in Zhejiang Province. More attention should be paid to maintaining relevant satisfaction with HBCCS to ensure positive mental health among rural and urban older adults.
Development and validation of a risk prediction model for dyslipidemia in community-dwelling middle-aged and older adults in China: a nationwide survey
Authors: Jiang Hailong , Geng Xiaoting , Shi Jie , Zhang Chi , Li Chang , Gai Ying , Mei Jia , Li Shuying 
Published: November 2024

BackgroundThe incidence of dyslipidemia as a risk factor for many serious diseases is increasing year by year. This study aimed to construct and visualize a risk prediction model for dyslipidemia in middle-aged and older adults.MethodThe subjects of our study are derived from CHARLS. Participants were allocated to training and validation groups in a 7:3 ratio at random. To identify potential predictors of dyslipidemia, we employed univariate analysis, lasso regression, and multivariate binary logistic regression analyses. A nomogram was constructed based on logistic regression results, and a ROC curve was used to evaluate its predictive performance. The accuracy and discriminatory capability were assessed using calibration curve analysis, while the net clinical benefit rate was evaluated through decision curve analysis (DCA).ResultsOur study included a total of 12,589 participants, of which 1,514 were detected with dyslipidemia syndrome. Model construction: Based on the results of the logistic regression analysis of the training set, six variables were selected to construct the model, which were ranked in order of importance as comorbid hypertension, comorbid diabetes, waistline, comorbid digestive disease, place of abode, and comorbid liver disease. The ROC curve results indicated that the prediction model exhibited moderate discriminatory ability (AUC &gt; 0.7). Additionally, the calibration curve confirmed the model's strong predictive accuracy. The decision curve analysis (DCA) illustrated a positive net benefit associated with the prediction model.ConclusionsThe prediction model of dyslipidemia risk in middle-aged and older adults constructed in this study has good efficacy and helps to screen high-risk groups.