bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative analysis of secreted protein disulfide isomerases from the tropical co-endemic parasites Schistosoma mansoni and Leishmania major
Authors: Miele Adriana E. , Badaoui Sofiane , Maugliani Lorenzo , Salza Romain , Boumis Giovanna , Chichiarelli Silvia , Duclos Bertrand , Ricard-Blum Sylvie 
Published: July 2019

AbstractThe human parasitesSchistosoma mansoniandLeishmania majorare co-endemic and a major threat to human health. Though displaying different tissue tropisms, they excrete/secrete similar subsets of intracellular proteins that, interacting with the host extracellular matrix (ECM), help the parasites invading the host. We selected one of the most abundant proteins found in the secretomes of both parasites, protein disulfide isomerase (PDI), and performed a comparative screening with surface plasmon resonance imaging (SPRi), looking for ECM binding partners. Both PDIs bind heparan sulfate; none of them binds collagens; each of them binds further ECM components, possibly linked to the different tropisms. We investigated by small-angle X-ray scattering both PDIs structures and those of a few complexes with host partners, in order to better understand the differences within this conserved family fold. Furthermore, we highlighted a previously undisclosed moonlighting behaviour of both PDIs, namely a concentration-dependent switch of function from thiol-oxidoreductase to holdase. Finally, we have tried to exploit the differences to look for possible compounds able to interfere with the redox activity of both PDI.
A novel group of avian astroviruses from Neotropical passerine birds broaden the diversity and host range of Astroviridae
Authors: Fernández-Correa Izaskun , Truchado Daniel A. , Gomez-Lucia Esperanza , Doménech Ana , Pérez-Tris Javier , Schmidt-Chanasit Jonas , Cadar Daniel , Benítez Laura 
Published: July 2019

AbstractMetagenomics is helping to expand the known diversity of viruses, especially of those with poorly studied hosts in remote areas. The Neotropical region harbors a considerable diversity of avian species that may play a role as both host and short-distance vectors of unknown viruses. Viral metagenomics of cloacal swabs from 50 Neotropical birds collected in French Guiana revealed the presence of four complete astrovirus genomes. They constitute an early diverging novel monophyletic clade within the Avastrovirus phylogeny, representing a putative new astrovirus species (provisionally designated as Avastrovirus 5) according to the International Committee on Taxonomy of Viruses (ICTV) classification criteria. Their genomic organization shares some characteristics with Avastrovirus but also with Mamastrovirus. The pan-astrovirus RT-PCR analysis of the cloacal samples of 406 wild Neotropical birds showed a community-level prevalence of 4.9% (5.1% in passerines, the highest described so far in this order of birds). By screening birds of a remote region, we expanded the known host range of astroviruses to the avian families Cardinalidae, Conopophagidae, Furnariidae, Thamnophilidae, Turdidae and Tyrannidae. Our results provide important first insights into the unexplored viral communities, the ecology, epidemiology and features of host-pathogen interactions that shape the evolution of avastroviruses in a remote Neotropical rainforest.
Characterisation of the Selective Reduced Uteroplacental Perfusion (sRUPP) Model of Preeclampsia
Authors: Morton J. S. , Levasseur J. , Ganguly E. , Quon A. , Kirschenman R. , Dyck J. R. B. , Fraser G. M. , Davidge S. T. 
Published: July 2019

AbstractPreeclampsia is a complication of pregnancy characterised by gestational hypertension, proteinuria and/or end organ disease. The reduced uteroplacental perfusion (RUPP) model, via partial occlusion of the lower abdominal aorta, mimics insufficient placental perfusion as a primary causal characteristic of preeclampsia. However, a major limitation of the RUPP model is that perfusion is reduced to the entire hindquarters of the rat resulting in hindlimb ischemia. We hypothesised that clipping the uterine and ovarian arteries in the selective (s)RUPP model would provoke signs of preeclampsia while avoiding systemic ischemia. Sham, RUPP or sRUPP procedures were performed in pregnant Sprague Dawley rats on gestational day (GD)14. On GD21 uterine blood flow was significantly reduced in both the RUPP and sRUPP models while aortic flow was reduced only in RUPP. Both models resulted in increased MAP, increased vascular oxidative stress (superoxide generation), increased pro-inflammatory (RANTES) and reduced pro-angiogenic (endoglin) mediators. Vascular compliance and constriction were unaltered in either RUPP or sRUPP groups. In summary, refinements to the RUPP model simultaneously maintain the characteristic phenotype of preeclampsia and avoid peripheral ischemia; providing a useful tool which may be used to increase our knowledge and bring us closer to a solution for women affected by preeclampsia.
New Ca. Liberibacter psyllaurous haplotype resurrected from a 49-year-old specimen of Solanum umbelliferum: a native host of the psyllid vector
Authors: Mauck Kerry Elizabeth , Sun Penglin , Meduri Venkata RamaSravani , Hansen Allison K. 
Published: July 2019

AbstractOver the last century, repeated emergence events within the Candidatus Liberibacter taxon have produced pathogens with devastating effects. Presently, our knowledge of Ca. Liberibacter diversity, host associations, and interactions with vectors is limited due to a focus on studying this taxon within crops. But to understand traits associated with pathogen emergence it is essential to study pathogen diversity in wild vegetation as well. Here, we explore historical native host plant associations and diversity of the cosmopolitan species, Ca. L. psyllaurous, also known as Ca. L. solanacearum, which is associated with psyllid yellows disease and zebra chip disease, especially in potato. We screened tissue from herbarium samples of three native solanaceous plants collected near potato-growing regions throughout Southern California over the last century. This screening revealed a new haplotype of Ca. L. psyllaurous (G), which, based on our sampling, has been present in the U.S. since at least 1970. Phylogenetic analysis of this new haplotype suggests that it may be closely related to a newly emerged North American haplotype (F) associated with zebra chip disease in potatoes. Our results demonstrate the value of herbarium sampling for discovering novel Ca. Liberibacter haplotypes not previously associated with disease in crops.
Temporal Stability of the Ruminal Bacterial Communities in Beef Steers
Authors: Clemmons Brooke A. , Martino Cameron , Schneider Liesel G. , Lefler Josh , Embree Mallory M. , Myer Phillip R. 
Published: July 2019

AbstractNutritional studies involving ruminants have traditionally relied on relatively short transition or wash-out periods between dietary treatments, typically two to four weeks. However, little is known about adequate adaptation periods required to reach stabilization of the rumen microbiome that could provide more accurate results from nutritional studies in ruminants. This study determined the rumen bacterial communities and rumen environment parameters over ten weeks following transition from a forage-based to concentrate-based diet. Several α-diversity metrics, including observed OTUs and Simpson’s Evenness fluctuated throughout the trial, but were typically either greatest (observed OTUs) or lowest (Simpson’s) at week 5 of the trial contrasted from weeks 1 and 10 (P < 0.05). At week 4, several orders associated with the shift to the final bacterial community composition, including Pasteurellales, Aeromonadales, and Bacteroidales. At week 5, rumen pH was correlated with α-diversity (P = 0.005) and predictive of the rumen microbiome signature at week 10 (R2 = 0.48; P = 0.04). Rumen microbiome stability did not occur until approximately 9 weeks following adaptation to the diet and was associated with changes in specific bacterial populations and rumen environment. The results of this study suggest that adaptation and wash-out periods must be re-evaluated in order to accommodate necessary rumen microbiome acclimation.
Epidemiological, Clinical and Genetic Study of Hypophosphatasia in A Spanish Population: Identification of Two Novel Mutations in The Alpl Gene
Authors: García-Fontana Cristina , Villa-Suárez Juan M. , Andújar-Vera Francisco , González-Salvatierra Sheila , Martínez-Navajas Gonzalo , Real Pedro J. , Gómez Vida José M. , de Haro Tomás , García-Fontana Beatriz , Muñoz-Torres Manuel 
Published: July 2019

AbstractHypophosphatasia (HPP) is a genetic disease caused by one or several mutations in ALPL gene encoding the tissue-nonspecific alkaline phosphatase affecting the mineralization process. Due to its low prevalence and lack of recognition, this metabolic disorder is generally confused with other more frequent bone disorders. An assessment of serum total alkaline phosphatase (ALP) levels was performed in 78,590 subjects. Pyridoxal-5′-phosphate (PLP) concentrations were determined and ALPL gene was sequenced in patients potentially affected by HPP. Functional validation of the novel mutations found was performed using a cell-based assay. Our results showed persistently low serum ALP levels in 0.12% of subjects. Among the studied subjects, 40% presented with HPP-related symptoms. Nine of them (~28%) had a history of fractures, 5 (~16%) subjects showed chondrocalcinosis and 4 (~13%) subjects presented with dental abnormalities. Eleven subjects showed increased PLP concentrations. Seven of them showed ALPL gene mutations (2 of the mutations corresponded to novel genetic variants). In summary, we identified two novel ALPL gene mutations associated with adult HPP. Using this protocol, almost half of the studied patients were diagnosed with HPP. Based on these results, the estimated prevalence of mild HPP in Spain could be up to double than previously reported.
Incidence and risk factors for Preeclampsia in a cohort of healthy nulliparous pregnant women: a nested case-control study
Authors: Mayrink Jussara , Souza Renato T. , Feitosa Francisco E. , Rocha Filho Edilberto A. , Leite Débora F. , Vettorazzi Janete , Calderon Iracema M. , Sousa Maria H. , Costa Maria L. , Baker Philip N. , Cecatti Jose G. ,   Preterm SAMBA study group, Parpinelli Mary A. , Fernandes Karayna G. , Guida José P. , Santana Danielly S. , Barbosa Ricardo M. , Galvao Rafael B. F. , Cassettari Bianca F. , Pfitscher Lucia , de Feitosa Daisy Lucena , Ferreira Júnior Elias Melo , Anacleto Danilo , Zotareli Vilma , Silva Marcia Alice 
Published: July 2019

AbstractThe objective of this study is to determine the incidence, socio-demographic and clinical risk factors for preeclampsia and associated maternal and perinatal adverse outcomes. This is a nested case-control derived from the multicentre cohort study Preterm SAMBA, in five different centres in Brazil, with nulliparous healthy pregnant women. Clinical data were prospectively collected, and risk factors were assessed comparatively between PE cases and controls using risk ratio (RR) (95% CI) plus multivariate analysis. Complete data were available for 1,165 participants. The incidence of preeclampsia was 7.5%. Body mass index determined at the first medical visit and diastolic blood pressure over 75 mmHg at 20 weeks of gestation were independently associated with the occurrence of preeclampsia. Women with preeclampsia sustained a higher incidence of adverse maternal outcomes, including C-section (3.5 fold), preterm birth below 34 weeks of gestation (3.9 fold) and hospital stay longer than 5 days (5.8 fold) than controls. They also had worse perinatal outcomes, including lower birthweight (a mean 379 g lower), small for gestational age babies (RR 2.45 [1.52–3.95]), 5-minute Apgar score less than 7 (RR 2.11 [1.03–4.29]), NICU admission (RR 3.34 [1.61–6.9]) and Neonatal Near Miss (3.65 [1.78–7.49]). Weight gain rate per week, obesity and diastolic blood pressure equal to or higher than 75 mmHg at 20 weeks of gestation were shown to be associated with preeclampsia. Preeclampsia also led to a higher number of C-sections and prolonged hospital admission, in addition to worse neonatal outcomes.
Factors Affecting Cell Biomass and Flavonoid Production of Ficus deltoidea var. kunstleri in Cell Suspension Culture System
Authors: Haida Zainol , Syahida Ahmad , Ariff Syed Mohd , Maziah Mahmood , Hakiman Mansor 
Published: July 2019

AbstractA study was conducted to establish in vitro culture conditions for maximum production of biomass and flavonoid content for Ficus deltoidea var. kunstleri, locally named as Mas Cotek, known to have a wide variety of potential beneficial attributes for human health. Size of initial inoculum, cell aggregate and initial pH value have been suggested to influent content of biomass and flavonoid for cell suspension culture in several plant species. In the present study, leaf explants were cultured by cell suspension culture procedures in MSB5 basal medium supplemented with predetermined supplements of 30 g/L sucrose, 2.75 g/L gelrite, 2 mg/L picloram and 1 mg/L kinetin with continuous agitation of 120 rpm in a standard laboratory environment. Establishment of cell suspension culture was accomplished by culturing resulting callus in different initial fresh weight of cells (0.10, 0.25, 0.50, 1.0, and 2.0 g/25 mL of media) using similar basal medium. The results showed that the highest production of biomass (0.65 g/25 mL of media) was recorded from an initial inoculum size of 2.0 g/25 mL media, whereas the highest flavonoid (3.3 mg RE/g DW) was found in 0.5 g/25 mL of media. Cell suspension fractions classified according to their sizes (500–750 µm, 250–500 µm, and <250 µm). Large cell aggregate size (500–750 µm) cultured at pH 5.75 produced the highest cell biomass (0.28 g/25 mL media) and flavonoid content (3.3 mg RE/g DW). The study had established the optimum conditions for the production of total antioxidant and flavonoid content using DPPH and FRAP assays in cell suspension culture of F. deltoidea var. kunstleri.
Tailored Medical Management of the Complex Postoperative Crohn's Disease Patient
Authors: Achkar Jean-Paul 
Published: July 2019

AbstractDespite advancements in medical therapy, many patients with Crohn's disease continue to require surgery for intestinal resection and/or management of perianal disease at some point in their disease course. Unfortunately, in this complex group of patients, postoperative disease recurrence rates are high. Medical prophylaxis can be used to prevent Crohn's disease recurrence or manage residual disease after surgery, but the ideal timing to start medications after surgery varies based on patient risk factors and patient preference for medication use. Currently, the largest medical treatment effects are seen with thiopurines and antitumor necrosis factor antibodies, but there are continually expanding options as new medical therapies are developed. A proposed algorithm stratified based on patient risk factors is provided.
Role of Fecal Diversion in Complex Crohn's Disease
Authors: Burke John P. 
Published: July 2019

AbstractCrohn's disease (CD) is a chronic inflammatory condition of the gastrointestinal tract resulting in progressive tissue damage, which can result in strictures, fistulae, and abscesses formation. The triggering mechanism is thought to be in the fecal stream, and diversion of this fecal stream is sometimes required to control disease when all other avenues of medical and surgical management have been exhausted. Fecal diversion can be temporary or permanent with the indications being defunctioning a high-risk anastomosis, as a result of a surgical complication, for disease control, or due to severe colonic, rectal, or perianal disease. The incidence of ostomy formation in CD has increased epidemiologically over time. The primary indication for ostomy formation is severe perianal fistulizing disease. However, while 64% of patients have an early clinical response after diversion for refractory perianal CD, restoration of bowel continuity is attempted in only 35% of patients, and is successful in only 17%. The current review discusses the indications for ostomy creation in complex CD, strategies for procedure selection, and patient outcomes.
Crohn's Disease and the Risk of Cancer
Authors: Carchman Evie 
Published: July 2019

AbstractCrohn's disease is associated with various intestinal and extraintestinal malignancies. This article reviews the current literature regarding Crohn's disease and subsequent risk of cancer formation. Recognition of risk factors (both modifiable and unmodifiable) is essential for prevention and appropriate screening. Future investigations into the molecular mechanisms associated with Crohn-related malignancy will provide additional insight into carcinogenesis, potential for early intervention, and identification of at-risk patients.
ERNICA Consensus Conference on the Management of Patients with Esophageal Atresia and Tracheoesophageal Fistula: Diagnostics, Preoperative, Operative, and Postoperative Management
Authors: Dingemann Carmen , Eaton Simon , Aksnes Gunnar , Bagolan Pietro , Cross Kate M. , De Coppi Paolo , Fruithof JoAnne , Gamba Piergiorgio , Husby Steffen , Koivusalo Antti , Rasmussen Lars , Sfeir Rony , Slater Graham , Svensson Jan F. , Van der Zee David C. , Wessel Lucas M. , Widenmann-Grolig Anke , Wijnen Rene , Ure Benno M. 
Published: July 2019

Introduction Many aspects of the management of esophageal atresia (EA) and tracheoesophageal fistula (TEF) are controversial and the evidence for decision making is limited. Members of the European Reference Network for Rare Inherited Congenital Anomalies (ERNICA) conducted a consensus conference on the surgical management of EA/TEF based on expert opinions referring to the latest literature.
Materials and Methods Nineteen ERNICA representatives from nine European countries participated in the conference. The conference was prepared by item generation, item prioritization by online survey, formulation of a final list containing the domains diagnostics, preoperative, operative, and postoperative management, and literature review. The 2-day conference was held in Berlin in October 2018. Anonymous voting was conducted via an internet-based system. Consensus was defined when 75% of the votes scored 6 to 9.
Results Fifty-two items were generated with 116 relevant articles of which five studies (4.3%) were assigned as level-1evidence. Complete consensus (100%) was achieved on 20 items (38%), such as TEF closure by transfixing suture, esophageal anastomosis by interrupted sutures, and initiation of feeding 24 hours postoperatively. Consensus ≥75% was achieved on 37 items (71%), such as routine insertion of transanastomotic tube or maximum duration of thoracoscopy of 3 hours. Thirteen items (25%) were controversial (range of scores, 1–9). Eight of these (62%) did not reach consensus.
Conclusion Participants of the conference reached significant consensus on the management of patients with EA/TEF. The consensus may facilitate standardization and development of generally accepted guidelines. The conference methodology may serve as a blueprint for further conferences on the management of congenital malformations in pediatric surgery.
Generational styles in oral storytelling
Authors: Gerstenberg Annette 
Published: July 2019

AbstractWhen it comes to autobiographical narratives, the most spontaneous and natural manner is preferable. But neither individually told narratives nor those grounded in the communicative repertoire of a social group are easily comparable. A clearly identifiabletertium comparationisis mandatory. We present the results of an experimental ‘Narrative Priming’ setting with French students. A potentially underlying model of narrating from personal experience was activated via a narrative prime, and in a second step, the participants were asked to tell a narrative of their own. The analysis focuses on similarities and differences between the primes and the students’ narratives. The results give evidence for the possibility to elicit a set of comparable narratives via a prime, and to activate an underlying narrative template. Meaningful differences are discussed as generational and age related styles. The transcriptions from the participants that authorized the publication are available online.
Biochemical characteristics of maltose phosphorylase MalE from Bacillus sp. AHU2001 and chemoenzymatic synthesis of oligosaccharides by the enzyme
Authors: Gao Yu , Saburi Wataru , Taguchi Yodai , Mori Haruhide 
Published: July 2019

ABSTRACT
Maltose phosphorylase (MP), a glycoside hydrolase family 65 enzyme, reversibly phosphorolyzes maltose. In this study, we characterized Bacillus sp. AHU2001 MP (MalE) that was produced in Escherichia coli. The enzyme exhibited phosphorolytic activity to maltose, but not to other α-linked glucobioses and maltotriose. The optimum pH and temperature of MalE for maltose-phosphorolysis were 8.1 and 45°C, respectively. MalE was stable at a pH range of 4.5–10.4 and at ≤40°C. The phosphorolysis of maltose by MalE obeyed the sequential Bi–Bi mechanism. In reverse phosphorolysis, MalE utilized d-glucose, 1,5-anhydro-d-glucitol, methyl α-d-glucoside, 2-deoxy-d-glucose, d-mannose, d-glucosamine, N-acetyl-d-glucosamine, kojibiose, 3-deoxy-d-glucose, d-allose, 6-deoxy-d-glucose, d-xylose, d-lyxose, l-fucose, and l-sorbose as acceptors. The kcat(app)/Km(app) value for d-glucosamine and 6-deoxy-d-glucose was comparable to that for d-glucose, and that for other acceptors was 0.23–12% of that for d-glucose. MalE synthesized α-(1→3)-glucosides through reverse phosphorolysis with 2-deoxy-d-glucose and l-sorbose, and synthesized α-(1→4)-glucosides in the reaction with other tested acceptors.
Optimal Techno-Economic Design of Standalone Hybrid Renewable Energy System Using Genetic Algorithm
Authors: Izdin.Hlal Mohamad , Ramachandaramurthy Vigna K , Hafiz Nagi Farrukh , bin Tuan Abdullah Tuan Ab Rashid 
Published: July 2019

Abstract
This paper presents a methodology to size Standalone Hybrid Renewable Energy System (SHRES) which combines solar PV, wind turbine (WT) and battery energy storage (BES) for application in rural areas. These sources are integrated via an AC bus to support the load demand. SHRES is simulated under varying load demand, solar radiation, temperature and wind speed obtained from the Malaysian Meteorological Department. A Multi-objective Optimization using Non-dominate Sorting Genetic Algorithm (NSGA-II) was utilized to determine the best sizing of PV / wind turbine / battery, and minimize Cost of Energy (COE) and Loss of Power Supply Probability (LPSP). The results show that the NSGAII optimization of the model is able to determine the best techno-economic sizing for the suggested location. For the case study, the optimum COE was 0.1099 (USD/kWh) and LPSP was 0.0865. The proposed tool can be used to size the SHRES for rural electrification and enhance energy access within remote locations.
Experimental Combined Grouping Analysis Approach for Robust Battery pack design for Electric Vehicles with Higher Performance
Authors: Yun Liu , Shui Li , Gao Liang , Fan Zhun , Ruhatiya C. , Wang Chin-Tsan , Garg Akhil 
Published: July 2019

Abstract
Battery consistency is an important factor for battery pack performance. Excellent battery consistency can make battery packs more energy efficient and electric vehicles can have longer mileage and higher safety. Thus, in this study a comprehensive intelligent clustering methodology for the design of Li-ion battery pack on the basis of uniformity and equalization criteria of the cell was proposed. Firstly, multiple parameters (capacity, voltage, temperature and resistance) test of single cell performance was performed. Secondly, a clustering method combine with self-organizing map neural network (SOM) was proposed. Furthermore, a validation experiment (pack level) was carried out to verify the accuracy of proposed clustering algorithm. It can be concluded that the battery pack formed from SOM sorting results perform better than the battery pack having random cells combination as well as the pack originally purchased from the manufacturer.