bluuebunny/crossref_metadata_2025_split huggingface dataset:
A metabolic perspective on CSF-mediated neurodegeneration in multiple sclerosis
Authors: Wentling Maureen , Lopez-Gomez Carlos , Park Hye-Jin , Amatruda Mario , Ntranos Achilles , Aramini James , Petracca Maria , Rusielewicz Tom , Chen Emily , Tolstikov Vladimir , Kiebish Michael , Fossati Valentina , Inglese Matilde , Quinzii Catarina M. , Katz Sand Ilana , Casaccia Patrizia 
Published: June 2019

AbstractMultiple sclerosis is an autoimmune demyelinating disorder of the CNS, characterized by inflammatory lesions and an underlying neurodegenerative process, which is more prominent in patients with progressive disease course. It has been proposed that mitochondrial dysfunction underlies neuronal damage, the precise mechanism by which this occurs remains uncertain. To investigate potential mechanisms of neurodegeneration, we conducted a functional screening of mitochondria in neurons exposed to the CSF of multiple sclerosis patients with a relapsing remitting (n = 15) or a progressive (secondary, n = 15 or primary, n = 14) disease course. Live-imaging of CSF-treated neurons, using a fluorescent mitochondrial tracer, identified mitochondrial elongation as a unique effect induced by the CSF from progressive patients. These morphological changes were associated with decreased activity of mitochondrial complexes I, III and IV and correlated with axonal damage. The effect of CSF treatment on the morphology of mitochondria was characterized by phosphorylation of serine 637 on the dynamin-related protein DRP1, a post-translational modification responsible for unopposed mitochondrial fusion in response to low glucose conditions. The effect of neuronal treatment with CSF from progressive patients was heat stable, thereby prompting us to conduct an unbiased exploratory lipidomic study that identified specific ceramide species as differentially abundant in the CSF of progressive patients compared to relapsing remitting multiple sclerosis. Treatment of neurons with medium supplemented with ceramides, induced a time-dependent increase of the transcripts levels of specific glucose and lactate transporters, which functionally resulted in progressively increased glucose uptake from the medium. Thus ceramide levels in the CSF of patients with progressive multiple sclerosis not only impaired mitochondrial respiration but also decreased the bioavailability of glucose by increasing its uptake. Importantly the neurotoxic effect of CSF treatment could be rescued by exogenous supplementation with glucose or lactate, presumably to compensate the inefficient fuel utilization. Together these data suggest a condition of ‘virtual hypoglycosis’ induced by the CSF of progressive patients in cultured neurons and suggest a critical temporal window of intervention for the rescue of the metabolic impairment of neuronal bioenergetics underlying neurodegeneration in multiple sclerosis patients.
Organoid-based ex vivo reconstitution of Kras-driven pancreatic ductal carcinogenesis
Authors: Matsuura Tetsuya , Maru Yoshiaki , Izumiya Masashi , Hoshi Daisuke , Kato Shingo , Ochiai Masako , Hori Mika , Yamamoto Shogo , Tatsuno Kenji , Imai Toshio , Aburatani Hiroyuki , Nakajima Atsushi , Hippo Yoshitaka 
Published: June 2019

AbstractThe organoid culture technique has been recently applied to modeling carcinogenesis in several organs. To further explore its potential and gain novel insights into tumorigenesis, we here investigated whether pancreatic ductal adenocarcinoma (PDA) could be generated as subcutaneous tumors in immunocompromised nude mice, by genetic engineering of normal organoids. As expected, acute induction of KrasG12Din vitro occasionally led to development of tiny nodules compatible with early lesions known as pancreatic intraepithelial neoplasia (PanIN). KrasG12D-expressing cells were enriched after inoculation in the subcutis, yet proved rather declined during culture, suggesting that its advantage might depend on surrounding environments. Depletion of growth factors or concurrent Trp53 deletion resulted in its robust enrichment, invariably leading to development of PanIN or large high-grade adenocarcinoma, respectively, consistent with in vivo mouse studies for the same genotype. Progression from PanIN was also recapitulated by subsequent knockdown of common tumor suppressors, whereas the impact of Tgfbr2 deletion was only partially recapitulated, illustrating genotype-dependent requirement of the pancreatic niche for tumorigenesis. Intriguingly, analysis of tumor-derived organoids revealed that KrasG12D-expressing cells with spontaneous deletion of wild-type Kras were positively selected and exhibited an aging-related mutation signature in nude mice, mirroring the pathogenesis of human PDA, and that the sphere-forming potential and orthotopic tumorigenicity in syngenic mice were significantly augmented. These observations highlighted the relevance of the subcutis of nude mice in promoting PDA development despite its ectopic nature. Taken together, pancreatic carcinogenesis could be considerably recapitulated with organoids, which would probably serve as a novel disease model.
A Novel, High-sensitivity, Bacteriophage-based Assay Identifies Low-level Mycobacterium tuberculosis Bacteremia in Immunocompetent Patients With Active and Incipient Tuberculosis
Authors: Verma Raman , Swift Benjamin M C , Handley-Hartill Wade , Lee Joanne K , Woltmann Gerrit , Rees Catherine E D , Haldar Pranabashis 
Published: June 2019

Abstract
The haematogenous dissemination of Mycobacterium tuberculosis (Mtb) is critical to the pathogenesis of progressive tuberculous infections in animal models. Using a novel, phage-based blood assay, we report the first concordant evidence in well-characterized, immunocompetent human cohorts, demonstrating associations of Mtb bacteremia with progressive phenotypes of latent infection and active pulmonary tuberculosis.
Species Richness and Community Composition of Ants and Beetles in Bt and non-Bt Maize Fields
Authors: Fernandes Marcos Gino , Costa Eduardo Neves , Dutra Carla Cristina , Raizer Josué 
Published: June 2019

AbstractInsect-resistant genetically modified (GM) plants have been cultivated in several countries on a large scale. These plants express the Cry toxins from the bacterium Bacillus thuringiensis Berliner (Bacillales: Bacillaceae) (Bt), which confers target-pest resistance to plants. Studies on the effects of GM plants on nontarget organisms are important to assess the technology’s impact on biodiversity. The objective of this study was to determine whether there are differences in the species richness and composition of ants and ground beetles in Bt maize (four different toxins: Cry1Ab, Cry1F, and Cry1A.105/Cry2Ab2) and conventional isoline (non-Bt) fields, in the first (summer) and second (winter) cropping seasons in 13 localities of Mato Grosso do Sul State, Brazil, over a 2-yr period (2009–2011). The assessment of Coleoptera and Formicidae species was performed using pitfall traps placed in fields throughout the period of vegetative growth and maturation of plants. Data were analyzed using faunistic indices of species richness, ordination by nonmetric multidimensional scaling, and multivariate analysis of variance. No statistically significant difference was found in the species richness of ant and ground beetle communities when comparing sites of Bt with those of non-Bt maize. Overall, Bt technology did not affect the composition of ant and ground beetles; however, municipality and cropping season exerted influence on the beetle composition. Some species were only observed in the first crop, whereas others were only observed in the second crop. This research suggests that Bt maize does not affect ant and ground beetle populations differently from its isoline.
Origins and Mechanisms of Social Influences in Couples: The Case of Retirement Decisions
Authors: Eismann Maria , Henkens Kène , Kalmijn Matthijs 
Published: June 2019

AbstractThe interdependence between partners raises considerable interest in the sociology of life course, work, and families. Partner influences play a particularly important role in the work domain, because each partner’s work decisions have profound effects on the couple as a whole. In contrast to previous research, this article pays detailed attention to the role a partner plays in workers’ labour market decisions by analysing the case of early retirement decisions. We hypothesized that partners’ preferences for older workers’ retirement originate from altruism and self-interest. Moreover, we expected that partners influence older workers’ early retirement behaviour via persuasion and pressure. To adequately estimate partners’ and workers’ preferences for the worker’s retirement, we used an instrumental variable approach. This was possible because we collected multi-actor longitudinal data from a large representative sample of older workers and their partners in the Netherlands. The results support that spousal preferences originate in altruism and self-interest and that partners influence workers through persuasion and pressure. Gender differences in origins and mechanisms of partner influence are also discussed.
Demographic differences in Canadian medical students’ motivation and confidence to promote physical activity
Authors: McFadden Taylor , Fortier Michelle , McGinn Ryan , Levac Brendan M , Sweet Shane N , Tomasone Jennifer R 
Published: June 2019

AbstractBackgroundPhysical activity (PA) remains under prescribed by physicians. Motivation and confidence are clear drivers of frequency of promoting PA. Research shows demographic differences in physicians’ preventive practices, yet none have included medical students who form habits during training.ObjectivesStudy objectives were to (i) examine how Canadian medical students’ motivation to recommend PA to future patients differs according to six demographic variables (i.e. gender, ethnicity, year of study, university, proposed specialty and academic background) and (ii) examine how Canadian medical students’ confidence to recommend PA to future patients differs according to these same demographic variables.MethodsA cross-sectional design was used. First to fourth year medical students from three medical schools responded to an online survey (N = 221).ResultsFemale participants were more motivated to counsel patients on PA and refer to an exercise specialist compared to males (P < 0.01). Second year students were more motivated to assess a patients’ level of PA compared to third and fourth year students (P < 0.01). Students pursuing family medicine were more confident to assess and counsel compared to students pursuing paediatrics (P < 0.01).ConclusionGiven that motivation and confidence have a positive influence on frequency-promoting PA, these results suggest where future efforts should focus, to improve PA promotion in medical practice. Physical inactivity continues to be a major issue worldwide, and medical students as future physicians have a unique opportunity to enhance PA amongst the population.
Occurrence and ecological determinants of the contamination of floodplain wetlands with Klebsiella pneumoniae and pathogenic or antibiotic-resistant Escherichia coli.
Authors: Henriot Charles P , Martak Daniel , Cuenot Quentin , Loup Christophe , Masclaux Hélène , Gillet François , Bertrand Xavier , Hocquet Didier , Bornette Gudrun 
Published: June 2019

ABSTRACT
The survival and multiplication of human pathogenic and antibiotic-resistant bacteria in ecosystems is of increasing concern but has been little explored. Wetlands can be contaminated by water fluxes from rivers and may present environmental conditions leading to bacterial survival and multiplication. To test this hypothesis, we sampled 16 wetlands located along three rivers of the Jura Massif, France. The bacterial contamination of the wetland and river waters was measured monthly over a one-year cycle together with the water physico-chemical characteristics. We assessed the abundance of three pathogenic species: Escherichia coli,Klebsiella pneumoniaeand Pseudomonas aeruginosa. The concentrations of E. coli producing extended-spectrum β-lactamase (ESBL E. coli) or belonging to the phylogenetic group B2 (E. coli B2–more pathogenic) were also measured. We found that rivers carried total E. coli, ESBL E. coli, and K. pneumoniae to wetlands. ESBL E. coli poorly survived in wetlands, whereas total E. coli and K. pneumoniae possibly met favourable physico-chemical conditions for survival and multiplication in these habitats. K. pneumoniae peaked in summer in warm and shallow wetlands. Total E. coli and E. coli B2 potentially reached wetlands through sources other than rivers (hillslope groundwater or leaching from contaminated fields).
Associations of perinatal characteristics with endometriosis: a nationwide birth cohort study
Authors: Gao Menghan , Scott Kirk , Koupil Ilona 
Published: June 2019

Abstract

Background
Perinatal characteristics are associated with subsequent risk of several chronic diseases. Previous studies regarding endometriosis were based on small samples and retrospective data and were limited by unmeasured confounding bias, leading to conflicting and inconclusive findings. We investigated the associations of maternal and birth characteristics with risk of endometriosis among Swedish women of reproductive age.

Methods
This total-population register-based cohort study consisted of 628 312 singleton women born in Sweden between 1973 and 1987, who were followed for diagnosed endometriosis from age 15 years until the end of 2012. Multivariable Cox regression was applied to examine associations with perinatal characteristics. Residual unmeasured confounding was assessed through within-family and E-value analyses.

Results
During follow-up, 8262 women received an endometriosis diagnosis. There were clear dose−response/linear associations of endometriosis with lower maternal education, endometriosis in the mother [adjusted hazard ratio (aHR): 2.24, 95% confidence interval (CI): 2.04–2.46], maternal smoking during pregnancy (aHR: 1.18, 95% CI: 1.04–1.33 for moderate smoker and aHR: 1.36, 95% CI: 1.18–1.57 for heavy smoker vs non-smoker), lower birthweight, and lower birthweight-for-gestational age (aHR: 0.93 per standard deviation increase, 95% CI: 0.91–0.95). Within-family and E-value analyses suggested that these perinatal characteristics are robust predictors of the incidence of endometriosis. We also found that an estimated 26% of the association between maternal smoking and early-onset endometriosis could be explained by birthweight-for-gestational age.

Conclusion
This study finds support for fetal origins of endometriosis, in that exposure to adverse environment or restricted development during the perinatal period may increase the risk. Further research is needed to provide an understanding of the underlying mechanisms.
Digging deeper in Shanghai: towards a ‘mechanism-rich’ epidemiology
Authors: Li Jie , Manning Nick , Mechelli Andrea 
Published: June 2019

Abstract

Background
There are very few close-up sociological or anthropological data informing epidemiological and psychiatric research design and/or contributing to our understanding of the relationship between mental health and specific forms of urban life. Furthermore, research on the relationships between urbanicity and mental disorder has paid little attention to the global diversity of urban experience, such as in cities in China, India and Brazil.

Methods
Two innovative methods can be employed to unveil the diversified urban experience of migrants in China, i.e. an ethnography-informed sociological deep surveying instrument and an ecological momentary assessment with a smartphone app. This article introduces the design and pilot survey of these new instruments towards a ‘mechanism-rich’ epidemiology.

Results
The ethnography-informed survey instrument enabled us to include some of the issues from the ethnography and successfully ‘dig deeper’ into respondents’ social experience. The pilot of the smartphone app serves as ‘proof of principle’ that we can recruit respondents in Shanghai, and that we can receive and use the data.

Conclusions
Both of these pilots have demonstrated good feasibility for studying mobility, urban life and mental health. Our next steps will be to extend the Shanghai sample, to use the app in Sao Paulo and Toronto and then hopefully in India and Africa.
Impact of TeamSTEPPS on patient safety culture in a Swiss maternity ward
Authors: Staines Anthony , Lécureux Estelle , Rubin Pascal , Baralon Christian , Farin Alexandre 
Published: June 2019

Abstract

Objective
To assess the impact of implementation of the TeamSTEPPS teamwork improvement concept on patient safety culture.

Design
Pre-post culture assessment using the Hospital Survey on Patient Safety Culture, at baseline and one year after implementation of TeamSTEPPS.

Setting
Two maternity wards within the same 480-bed multisite teaching hospital.

Intervention
Implementation of the TeamSTEPPS teamwork improvement concept.

Main Outcome Measures
Analysis of variation of the percentage of positive responses (score) in both wards (intervention and control) was conducted.

Results
There was a significant increase in scores in three dimensions of patient safety culture in the intervention ward: Supervisor/Manager Expectations and Actions Promoting Safety increased from 48.7% in 2015 to 70.8% in 2016 (P &lt; 0.005); Teamwork Within Units increased from 35.5% in 2015 to 54.5% in 2016 (P &lt; 0.005); Nonpunitive Response to Errors increased from 16.7% in 2015 to 32.3% in 2016 (P &lt; 0.005). Other dimensions showed no significant changes. In the control ward, there was a significant decrease in scores in one dimension. A secondary analysis of differences in differences still shows significant improvement in one dimension (Supervisor/Manager Expectations and Actions Promoting Safety P &lt; 0.005).

Conclusion
After implementing the TeamSTEPPS teamwork concept, patient safety culture significantly improved for three of twelve dimensions in the intervention group. When controlling for differences in baseline scores between implementation and control wards, a significant improvement remains in one dimension. This suggests that TeamSTEPPS could be considered when seeking to enhance patient safety culture, especially in high-risk environments such as maternity wards.
Comparison of antimicrobial resistance patterns in Streptococcus pneumoniae from respiratory and blood cultures in Canadian hospitals from 2007–16
Authors: Golden Alyssa R , Baxter Melanie R , Davidson Ross J , Martin Irene , Demczuk Walter , Mulvey Michael R , Karlowsky James A , Hoban Daryl J , Zhanel George G , Adam Heather J ,   Canadian Antimicrobial Resistance Alliance (CARA) and CANWARD, Zhanel George G , Hoban Daryl J , Adam Heather J , Baxter Melanie R , Nichol Kimberly A , Lagacé-Wiens Philippe R S , Walkty Andrew , Karlowsky James A , Blondeau J , Slinger R , Davidson R , Zhanel G , Hoban D , Delport J , Ellis C , Laverdière M , Loo V , Poutanen S , Fuller J , Roscoe D , Desjardins M , Matukas L , Goyette M , Lee C , Carignan A , Bergevin M , Pelletier R 
Published: June 2019

AbstractObjectivesTo compare the epidemiology and antimicrobial susceptibility patterns of Streptococcus pneumoniae collected from respiratory and blood culture samples in Canada between 2007 and 2016.MethodsS. pneumoniae strains were obtained from Canadian hospitals as part of the ongoing national surveillance study, CANWARD. Isolates were serotyped using the Quellung method. Antimicrobial susceptibility testing was performed using the CLSI broth microdilution method. MDR and XDR were defined as resistance to three or more and five or more classes of antimicrobials, respectively.ResultsOf the 2581 S. pneumoniae isolates collected, 1685 (65.3%) and 896 (34.7%) were obtained from respiratory and blood samples, respectively. Respiratory isolates demonstrated lower rates of antimicrobial susceptibility than blood isolates to penicillin, ceftriaxone, clarithromycin, clindamycin, doxycycline and trimethoprim/sulfamethoxazole (P ≤ 0.03). From 2007 to 2016, invasive isolates demonstrated trends towards increasing penicillin susceptibility and decreasing clarithromycin susceptibility. MDR was significantly higher in respiratory S. pneumoniae compared with blood (9.1% versus 4.5%, P < 0.0001). Serotypes 11A, 16F, 19F, 23A/B/F, 34, 35B and non-typeable strains were more commonly isolated from respiratory specimens, while 4, 5, 7F, 8, 12F, 14 and 19A were more commonly invasive serotypes. Numerous serotypes, including 3 and 22F, were isolated frequently from both specimen sources.ConclusionsS. pneumoniae from respiratory samples demonstrated lower antimicrobial susceptibilities and higher MDR in a greater diversity of serotypes than isolates obtained from blood. Many serotypes were associated with one specific specimen source, while others were associated with both; genetic characterization is necessary to elucidate the specific factors influencing the ability of these serotypes to commonly cause both invasive and non-invasive disease.
Satellite images and machine learning can identify remote communities to facilitate access to health services
Authors: Bruzelius Emilie , Le Matthew , Kenny Avi , Downey Jordan , Danieletto Matteo , Baum Aaron , Doupe Patrick , Silva Bruno , Landrigan Philip J , Singh Prabhjot 
Published: June 2019

Abstract

Objective
Community health systems operating in remote areas require accurate information about where people live to efficiently provide services across large regions. We sought to determine whether a machine learning analyses of satellite imagery can be used to map remote communities to facilitate service delivery and planning.

Materials and Methods
We developed a method for mapping communities using a deep learning approach that excels at detecting objects within images. We trained an algorithm to detect individual buildings, then examined building clusters to identify groupings suggestive of communities. The approach was validated in southeastern Liberia, by comparing algorithmically generated results with community location data collected manually by enumerators and community health workers.

Results
The deep learning approach achieved 86.47% positive predictive value and 79.49% sensitivity with respect to individual building detection. The approach identified 75.67% (n = 451) of communities registered through the community enumeration process, and identified an additional 167 potential communities not previously registered. Several instances of false positives and false negatives were identified.

Discussion
Analysis of satellite images is a promising solution for mapping remote communities rapidly, and with relatively low costs. Further research is needed to determine whether the communities identified algorithmically, but not registered in the manual enumeration process, are currently inhabited.

Conclusions
To our knowledge, this study represents the first effort to apply image recognition algorithms to rural healthcare delivery. Results suggest that these methods have the potential to enhance community health worker scale-up efforts in underserved remote communities.