bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heavy metal contamination and potential health risks in upland rice-producing soils of rotational shifting cultivation in northern Thailand
Authors: Arunrat Noppol , Kongsurakan Praeploy , Sereenonchai Sukanya 
Published: November 2024

AbstractRotational shifting cultivation (RSC) is commonly practiced in northern Thailand for upland rice cultivation, primarily for household consumption. However, the potential health risks from heavy metal contamination in these soils have not been thoroughly explored. This study aimed to evaluate the contamination of six heavy metals (Arsenic (As), Mercury (Hg), Cadmium (Cd), Lead (Pb), Copper (Cu), and Chromium (Cr)) in upland rice across RSC fields with varying fallow periods and assess the associated health risks from rice consumption. Four RSC fields with 5, 6, 10, and 12-year fallow periods were examined. The RSC-6Y and RSC-12Y fields were used for upland rice cultivation in 2022, while the RSC-5Y and RSC-10Y fields were cultivated in 2023. The geo-accumulation index (Igeo) was calculated, and translocation factors (TF) were assessed for the transfer of heavy metals from soil to straw (TFStraw/Soil), straw to grain (TFGrain/Straw), and soil to grain (TFGrain/Soil). The results indicated that after burning vegetation in the RSC fields, the highest concentrations of Pb, Cr, and Hg were found in the ash. In RSC soils, Cu, Cr, As, Pb, and Hg levels were below reference standards, with Cd undetected. In rice grains, the order of concentration was Pb > Cu > Cr > As, with Hg and Cd undetected. Pb levels in rice grains exceeded the safety threshold. Igeo values indicated no contamination to moderate contamination across sites, with negative Igeo values for Cr and Cu, and zero values for Cd. The TF results showed limited transfer of As, Hg, and Cd from soil to rice plants (TFStraw/Soil < 0.1), but notable transfer for Pb, Cr, and Cu. Pb was readily transferred from soil to grain (TFGrain/Soil), posing a potential health risk. The study highlights potential non-carcinogenic and carcinogenic risks from heavy metal exposure, particularly Pb, and underscores the need for further research to determine contamination sources and mitigation strategies.
Urinary sediment mRNA as a potent biomarker of IgA nephropathy
Authors: Kim Jin Sug , Kim Geon Woo , Hwang Hyeon Seok , Kim Yang Gyun , Moon Ju-Young , Lee Sang Ho , Seok Junhee , Tae Donghyun , Jeong Kyung Hwan 
Published: November 2024

Abstract
Background
The quantification of mRNA expression in urinary sediments is a reliable biomarker for various diseases. However, few studies have investigated the clinical relevance of urinary mRNA levels in IgA nephropathy (IgAN). Thus, we investigated the expression of urinary mRNAs and their clinical significance in IgAN.

Methods
Overall, 200 patients with biopsy-proven IgAN, 48 disease controls, and 76 healthy controls were enrolled. We identified the differential expression of mRNAs in renal tissue between patients with IgAN and normal subjects using the Gene Expression Omnibus dataset and selected candidate mRNAs. mRNA expression in the urinary sediment was measured using quantitative real-time polymerase chain reaction. Associations between urinary mRNA levels and clinicopathological parameters were analyzed and the predictive value of mRNAs for disease progression was evaluated.

Results
The urinary expression of CCL2, CD14, DNMT1, FKBP5, Nephrin, and IL-6 was significantly upregulated in patients with IgAN compared with healthy controls. C3, FLOT1, and Podocin levels were significantly correlated with renal function, where C3, FLOT1, and TfR levels were significantly correlated with urinary protein excretion. During follow-up, 26 (13.0%) patients with IgAN experienced disease progression, defined as a greater than 50% reduction in the estimated glomerular filtration rate or progression to end-stage renal disease. Urinary mRNA levels of FLOT1 (HR 3.706, 95% CI 1.373–10.005, P = 0.010) were independently associated with an increased risk of disease progression.

Conclusions
Our results suggest that urinary sediment mRNAs are a useful biomarker in IgAN patients. Further studies with larger sample sizes and longer follow-up durations are required.
NSDHL contributes to breast cancer stem-like cell maintenance and tumor-initiating capacity through TGF-β/Smad signaling pathway in MCF-7 tumor spheroid
Authors: Yoon So-Hyun , Lee Sangeun , Kim Hoe Suk , Song Junhyuk , Baek Moonjou , Ryu Seungyeon , Lee Han-Byoel , Moon Hyeong-Gon , Noh Dong-Young , Jon Sangyong , Han Wonshik 
Published: November 2024

Abstract
Background
NAD(P)-dependent steroid dehydrogenase-like protein (NSDHL), which is involved in breast tumor growth and metastasis, has been implicated in the maintenance of cancer stem cells. However, its role in regulating breast cancer stem-like cells (BCSCs) remains unclear. We have previously reported the clinical significance of NSDHL in patients with estrogen receptor-positive (ER +) breast cancer. This study aimed to elucidate the molecular mechanisms by which NSDHL regulates the capacity of BCSCs in the ER + human breast cancer cell line, MCF-7.

Methods
NSDHL knockdown suppressed tumor spheroid formation in MCF-7 human breast cancer cells grown on ultralow-attachment plates. RNA sequencing revealed that NSDHL knockdown induced widespread transcriptional changes in the MCF-7 spheroids. TGF-β signaling pathway was the most significantly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway (fold change ≥ 2, P ≤ 0.05) identified in NSDHL-knockdown MCF-7 spheroids compared with the control. In orthotopic tumor models injected with NSDHL-knockdown MCF-7 spheroids, tumor initiation and growth were strongly suppressed compared with those in the control.

Results
BCSC populations with CD44+/CD24- and CD49f+/EpCAM + phenotypes and high ALDH activity were decreased in NSDHL-knockdown MCF-7 spheroids and xenograft tumors relative to controls, along with decreased secretion of TGF-β1 and 3, phosphorylation of Smad2/3, and expression of SOX2. In RNA-sequencing data from The (TCGA) database, a positive correlation between the expression of NSDHL and SOX2 was found in luminal-type breast cancer specimens (n = 998). Our findings revealed that NSDHL plays an important role in maintaining the BCSC population and tumor-initiating capacity of ER-positive MCF-7 spheroids, suggesting that NSDHL is an attractive therapeutic target for eliminating BCSCs, thus preventing breast cancer initiation and progression.

Conclusions
Our findings suggest that NSDHL regulates the BCSC/tumor-initiating cell population in MCF-7 spheroids and xenograft tumors.
How to combat stigma surrounding mental health disorders: a scoping review of the experiences of different stakeholders
Authors: Hajizadeh Alireza , Amini Homayoun , Heydari Mahdiyeh , Rajabi Fatemeh 
Published: November 2024

Abstract
Background
The stigma associated with mental health disorders (MHDs) results in delayed help-seeking, limited access to health services, suboptimal treatment, poor treatment outcomes, and an increased risk of human rights violations. This scoping review summarizes qualitative research on the lived experiences of different stakeholders regarding strategies and interventions to combat stigma for people with MHDs.

Methods
This study was a six-step scoping review using the Arksey and O’Malley framework. A comprehensive search of the following electronic databases was conducted to identify relevant records: PubMed, Scopus, Web of Science (WoS) and Google Scholar, as well as a manual search of the reference lists. All steps, including screening of eligible studies, data extraction, and analysis, were performed independently by multiple reviewers, with disagreements resolved by discussion. The data were synthesized based on the for-content synthesis guidelines.

Results
A total of 25 studies were included in this review of the 32,976 initial identified citations. The included studies were from all countries (low, middle, and high income), stigmatized disorders (e.g., schizophrenia, bipolar disorder, etc.) and target populations (e.g., people with MHDs and their families, health care providers, the general community, and students and school members). The thematic synthesis revealed six types of interventions and strategies and 17 themes related to reducing stigma in patients on MHDs. Strategies and interventions were classified by patient (self-stigma), family (family stigma), healthcare professionals’ stigma, workplace stigma, public/societal stigma, and structural type of stigma (institutional stigma).

Conclusions
This review contributes new evidence that should be considered in future interventions and policies to reduce stigma against MHDs. Multilevel and multistakeholder strategies and interventions are needed to reduce the stigmatization of MHDs.
Health promoting resources and lifestyle factors among higher education students in healthcare and social work programmes: a survey with a longitudinal multicentre design
Authors: Ekman Aimée , Pennbrant Sandra , Sterner Anders , Forsberg Elenita , Hedén Lena , Nunstedt Håkan , Sundler Annelie J. , Larsson Margaretha , Larsson Ingrid , Ahlstrand Inger , Andersson Hammar Isabelle , Lood Qarin , Hallgren Jenny 
Published: November 2024

Abstract
Background
It has been suggested that the university environment, to improve students’ health status and educational outcomes, should be based on a health promoting approach. More knowledge is needed about health promoting resources and lifestyle factors that may be of value for students in higher education and their future work-life balance. The aim of this study was to explore health-promoting resources, general health and wellbeing, and health promoting lifestyle factors among fourth and final semester students in higher education in healthcare and social work.

Methods
This longitudinal study is based on self-reported data collected through a web-based questionnaire that included questions about general health, wellbeing, and healthy lifestyle factors and made use of instruments: the Sense of Coherence (SOC) scale, the Salutogenic Health Indicator Scale (SHIS), and five questions from the General Nordic Questionnaire (QPS Nordic). The questionnaire was distributed among students enrolled in seven different healthcare and social work programmes at six universities in Sweden. Data was collected when students were in their fourth (2019/2020) and final (2020/2021) semesters analysed with multiple linear and logistic regressions.

Results
The survey included responses from students during the fourth (n = 498) and the final (n = 343) semester of higher education programmes in health and social work. Total SOC scores decreased between the fourth semester and the final semester. The prevalence of the health promoting lifestyle factor of physical exercise decreased between the fourth and final semesters. Students in their final semester reported valuing group work more highly than did students in their fourth semester. Despite this, students in both the fourth and the final semester reported high SOC, low levels of good general health and perceived wellbeing, and sleeping problems.

Conclusions
Students’ report of good general health were associated with wellbeing, high-intensity physical training, and no sleeping problems A high SOC level was associated with good general health, perceived wellbeing, and no sleeping problems. A higher SHIS level was also associated with wellbeing and no sleeping problems. Therefore, we suggest further research focusing on how to prepare students in healthcare and social work during higher education for a future work-life in balance targeting effects on sleep quality.
Study design of herbal medicine clinical trials: a descriptive analysis of published studies investigating the effects of herbal medicinal products on human participants
Authors: Koonrungsesomboon Nut , Sakuludomkan Chotiwit , Na Takuathung Mingkwan , Klinjan Preeyaporn , Sawong Suphunwadee , Perera Pathirage Kamal 
Published: November 2024

Abstract
Background
Increasing global interest in natural therapies has led to a rise in the use of herbal medicines for managing various ailments. However, concerns about scientific evaluation have prompted a study aiming to assess the study design of herbal medicine clinical trials. This study aimed to provide a descriptive overview of the study design, characteristics, and methodologies of contemporary herbal medicine clinical trials.

Materials and methods
The study reviewed herbal medicine clinical trials published between 2019 and 2022 in five electronic databases: PubMed, Embase, Web of Sciences, Scopus, and the Cochrane Library. Data extraction included study characteristics, intervention details, study design, outcome measures, trial phases, blinding, and other relevant information, with descriptive analyses presented. The term ‘herbal medicines’ in this study refers to herbs, herbal materials, preparations, and finished products containing active ingredients from plant parts or their combinations.

Results
Out of the initially identified 5,918 records, 1,517 articles were eligible for inclusion in the study. The majority of herbal medicine clinical trials were conducted in Asian countries, covering a range of diseases. A randomized, double-blind, parallel design with a 1:1 allocation ratio was frequently employed, along with the common use of placebos across all trial phases. Capsules were the most common dosage form. The median number of human participants varied across trial phases, ranging from 50 in Phase 1 to 240 in Phase 4.

Conclusions
The analysis observed that herbal medicine clinical trials employed randomized, double-blind, parallel designs, and the widespread use of placebo. Our observations provided valuable insights into the evolving landscape of herbal medicine clinical trials.
What is known about persons with co-occurring problems’ experiences with supported housing, recovery, and health promotion? A scoping review
Authors: Hammervold Unn Elisabeth , Gytri Silje , Storm Marianne , Lid Torgeir Gilje , Sagvaag Hildegunn 
Published: November 2024

Abstract
Background
Having a home is the foundation of most people’s lives. People with co-occurring substance use and mental health problems may experience challenges in acquiring and keeping housing. Many also have major health challenges. Supported housing is the subject of increasing interest, but there seems to be a lack of studies exploring supported housing’s potential for facilitating recovery and health promotion. Therefore, a scoping review was performed to answer our review question: What is known in the literature about the experiences of persons with co-occurring substance use and mental health problems with supported housing, including experiences of recovery and health promotion?

Methods
Systematic searches were conducted in the Ovid MEDLINE, Embase, PsycInfo, CINAHL, Social Services Abstracts, Web of Science, Scopus, and Oria, and Idunn.no databases. The search terms were derived from the population, concepts, and context. The search for grey literature was conducted in various Norwegian sources.

Results
Forty studies were included: 7 with quantitative design, 28 with qualitative design and 5 with mixed methods design. The studies were from Canada, Ireland, Norway, Scotland, and the USA.
The review identified four themes related to tenants’ experiences with supported housing:
1)The importance of a permanent and safe home; 2) Housing’s importance for physical health; 3) A shoulder to lean on – the importance of relationships and support; 4) the value of choice and independence.
Factors that may influence physical health were poorly represented.

Conclusions
Long-term housing and safety are prerequisites for recovery for people with co-occurring problems. Programmes such as Housing First and Assertive Community Teams, especially, were experienced to support recovery. Autonomy was valued, including access to individual and respectful support from service providers when needed.
Supported housing may be a health-promoting arena, especially in relation to mental health. More attention should be given to how service providers can support tenants to protect their physical health, especially related to nutrition, meals and communal cooking. Further research is needed to tailor optimal services and support for people with co-occurring problems, including balancing support and autonomy with the aim of promoting health and recovery.
Peer specialists’ contributions to supported housing are scarce and need further development.
The journey to diagnosis of wild-type transthyretin-mediated (ATTRwt) amyloidosis: a path with multisystem involvement
Authors: Karam Chafic , Moffit Colleen , Summers Catherine , Merkel Madeline P. , Kochman Fran M. , Weijers Laure , Puls Mathilde , Schurer Marieke , Jones Emily , Mason Nicola , Finkel Muriel , Schmitt Paula , Hanna Mazen 
Published: November 2024

Abstract
Background
Wild-type and hereditary transthyretin-mediated amyloidosis (ATTRwt and ATTRv amyloidosis, respectively) are progressive, fatal diseases with a broad range of clinical presentations and multisystem effects. Despite having a higher prevalence, ATTRwt amyloidosis is less well characterized due to its non-hereditary nature, and its relatively poorer disease awareness delays diagnosis. Understanding of its natural history has evolved in recent years, but this is largely based on physician-collected data rather than patients’ reports of their own experiences. A mixed methods approach was used to evaluate how the healthcare journeys of patients with ATTRv and ATTRwt amyloidosis compare.

Methods
A quantitative survey was administered to US-patients diagnosed with both ATTRwt amyloidosis and ATTRv amyloidosis identified through a patient support group. Subsequent in-depth interviews with participants with ATTRwt amyloidosis were conducted. Quantitative data with related qualitative quotes from patients were produced to characterize their paths to diagnosis and the disease burden experienced.

Results
A total of 47 respondents completed the survey (ATTRv, n = 20 and ATTRwt, n = 27) and a total of 14 survey respondents with ATTRwt amyloidosis were interviewed. Survey results reported a high disease burden for patients with both conditions, with patients with ATTRwt amyloidosis reporting more diagnoses and procedures prior to their final diagnosis. Interviews with participants with ATTRwt amyloidosis revealed that patients face a high symptomatic burden of disease. Diagnosis was often delayed due to three key factors: (1) early signs of ATTRwt amyloidosis were often assumed to be related to old age; (2) many medical specialists working in silos were involved in participants’ diagnostic; and (3) there was a general lack of disease awareness. Early indicators such as carpal tunnel syndrome were often overlooked. Participants were typically diagnosed after the disease had progressed to include severe cardiac symptoms such as atrial fibrillation and severe shortness of breath. Sleep apnoea was also reported by a number of participants, with a considerable impact on quality of life.

Conclusions
Our study provides insight into the overall impact of the patient journey on their quality of life and demonstrates how increased awareness of ATTRwt amyloidosis and more coordinated engagement with physicians could reduce the time to diagnosis.
Variability of Loa loa microfilarial counts in successive blood smears and its potential implication in drug-related serious adverse events
Authors: Lepage Tristan M. , Campillo Jérémy T. , Louya Frédéric , Bikita Paul , Missamou François , Hemilembolo Marlhand C. , Pion Sébastien D. S. , Boussinesq Michel , Chesnais Cédric B. 
Published: November 2024

Abstract
Background
The standard method to diagnose Loa loa infection and quantify microfilarial density (MFD) is the microscopic examination of calibrated thick blood smears (TBSs). In 1950, it was noticed that successive L. loa MFD samples from a single capillary puncture could exhibit up to 20% variation. Although loiasis treatment allocation is based on MFD to prevent serious adverse events (SAEs), data on this variability are scarce. There are also no guidelines supporting the collection and analysis of one or two TBSs.

Methods
We assessed the variability of two successive L. loa MFD samples (MFD1 and MFD2), collected from 255 patients. We analyzed the influence of sex, age, weight, heart rate, arterial pressure, body temperature, and sampling time on MFD variability, as well the impact of MFD variability on MFD thresholds relevant to loiasis treatment protocols.

Results
The MFD2 was found to have increased in 63% (1145/1826) of TBS pairs and to have decreased in 37% (681/1826) of TBS pairs. The MFD2 were on average 28% higher than the MFD1. These variations drove a total of 333 (17.4%) changes in MFD classes according to loiasis treatment protocol, including 210 (11.3%) class increases. TBSs generated from blood samples from subjects with lower MFD (1–1000 mf/ml) or lower mean arterial pressure (MAP; 55–80 mmHg), or from blood samples collected at an earlier hour time-point (10:00–10:59 a.m.) were more subject to MFD2 variability in a multivariate analysis. The MFD relative change was not constant over time for a given person.

Conclusions
We observed a trend towards an increase in MFD2 with an important variability between samples that may impact loiasis treatment allocation. We suggest that systematically sampling at least two successive TBSs might allow better MFD assessments to prevent post-treatment SAEs. Further studies are needed to verify this variability in larger samples as well as confirm the potential explanatory variables identified.

Graphical Abstract
Disseminated histoplasmosis from western Mexico—rethinking our geographic distribution of endemic fungal species: a case report and review of literature
Authors: Sleightholm Richard , Hodson Daniel Z. , So Isabella , Avula Harshika , Batra Jagmohan 
Published: November 2024

Abstract
Background
Histoplasma is a fungal pathogen found in many parts of the world. In North America, its distribution is traditionally thought to be endemic to the Ohio and Mississippi River valleys. Development of histoplasmosis after Histoplasma exposure is related to degree of inoculum exposure and susceptibility, for example, immunocompromised status. Most exposed, healthy individuals are asymptomatic and few develop pulmonary symptoms. A limited number of infectious etiologies (that is, Histoplasma, Coccidioides, and Mycobacterium tuberculosis) can cause miliary pattern on chest imaging, and thus, histoplasmosis should be considered whenever a patient presents with pulmonary symptoms and these unique radiographic findings.

Case presentation
A previously healthy 13-year-old Hispanic male presented as a transfer from an outside hospital with fever and hypoxia in the setting of a progressive, subacute gastrointestinal illness. Given hypoxia, the concern for sepsis, and unclear etiology of his illness, broad-spectrum antimicrobial therapy and noninvasive ventilation were started. Initial evaluation demonstrated miliary pulmonary infiltrates, and travel history raised suspicion for coccidioidomycosis or tuberculosis. After a complete evaluation, lab studies confirmed a diagnosis of histoplasmosis, and the patient made a full recovery after the initiation and completion of antifungal therapy.

Conclusion
Herein, we present a patient who acquired histoplasmosis from an area of Mexico not currently acknowledged as endemic and review recently published data emphasizing new areas of Histoplasma endemicity in North America, particularly the southwest USA and most states of Mexico. Though limited surveillance data exist, mounting case reports/series and local epidemiologic studies illustrate the expanding worldwide endemicity of Histoplasma and underscore histoplasmosis as a growing global health concern.