bluuebunny/crossref_metadata_2025_split huggingface dataset:
APRI Profile of Cirrhotic Patients with Positive HBsAg
Authors: Husni Nadya , Anniwati Leonita , Lukitasari Lina 
Published: September 2019

Introduction: Hepatitis B is a viral infection that has the potency to become chronic infection and cause serious complications such as liver cirrhosis and hepatocellular carcinoma. One of the tools in diagnosing hepatitis B or cirrhosis and predicting the prognosis is aspartate aminotransferase to platelet ratio index (APRI). The purpose of this study is to evaluate the profile of APRI among cirrhotic patients with positive hepatitis B surface antigen (HBsAg).Method: This research was a descriptive observational study. The number of samples was 35 cirrhotic patients with positive HBsAg in RSUD Dr. Soetomo Surabaya from January-December 2017.Results: The majority of cirrhotic patients had >1,5 APRI score (48,57%). The most prevalent APRI score in Child A patients for first classification was 0,5 – 1,5 (5,71%) while for second classification was 0,7 – 1,5 (5,71%). The most prevalent APRI score in Child B patients for first classification was 0,5 – 1,5 (17,14%) while for second classification was 0,7 – 1,5 (11,43%). Most of Child C patients had >1,5 APRI score (22,86%). The majority of malignant degeneration patients also had >1,5 APRI score (14,29%).Conclusion: The majority of cirrhotic patients had >1,5 APRI score. In cirrhotic patients with Child A or B classification, the increase of APRI score was not as much as those with Child C or malignant degeneration classification.Keywords: Hepatitis B, cirrhosis, liver, hepatocellular carcinoma, APRI
The Relationship between Early Initiation of Breastfeeding and Exclusive Breastfeeding in the Work Area of Wiyung Public Health Center, Surabaya
Authors: Nisrina Vira Dwi , Wiyasihati Sundari Indah , Fatmaningrum Widati , Sulistiawati Sulistiawati 
Published: September 2019

Introduction: In Indonesia, there has been a 10% increase in the span of five years for exclusive breastfeeding, however this is still far from the national target. One of the factors that can cause low rates of national exclusive breastfeeding is that not all babies have received an early initiation of breastfeeding. The purpose of this study was to determine the relationship between early initiation of breastfeeding and exclusive breastfeeding for babies aged 7-12 months in the work area of Wiyung Public Health Center, Surabaya. Methods: This study was a quantitative research with a cross-sectional approach. The study used a questionnaire as the research instrument given to 30 respondents, namely mothers who had 7-12 month old babies in the work area of Wiyung Public Health Center, Surabaya. Results: The percentage of early initiation of breastfeeding in babies aged 7-12 months was 53.33% meanwhile the percentage of exclusive was 73.33%. Using Chi Square Fisher Exact Test, it was found that there is no relationship between early initiation of breastfeeding and exclusive breastfeeding for babies aged 7-12 months in the working area of the Wiyung Public Health Center in Surabaya. (The value of p = 0.574 with a value of α = 0.05, p>α). Conclusion: The early initiation of breastfeeding is not the only factor influencing exclusive breastfeeding for babies aged 7-12 months in the work area of the Wiyung Public Health Center in Surabaya. Further research is needed on other factors that can relate to and influence exclusive breastfeeding.
Correlation between Patient’s Age and Advanced Response Time with Outcome of Cardio Pulmonary Resuscitation in Cardiac Arrest Patient
Authors: Setiarini Debie , Kriswidyatomo Prihatma , Sari Gadis Meinar 
Published: September 2019

Introduction: This study is conducted to determine the correlation between patient’s age and advanced response time with the outcome of Cardio Pulmonary Resuscitation in cardiac arrest patients. Methods: This was an analytic observational cross-sectional study, evaluating correlation between patient’s age and advanced response time with the outcome of Cardio Pulmonary Resuscitation in cardiac arrest patients. This study used the primary data from Cardio Pulmonary Resuscitation sheets from February to August 2018 at Dr. Soetomo General Hospital, Surabaya. The data were analysed with Chi Square and Contingency Coefficient using SPSS version 22.0. Results: The results showed that 84.6% cardiac arrest patients who experience ROSC are over 40 years old, and 92.3% cardiac arrest patients who experience ROSC have advanced response time ≤ 5 minutes. After being tested using Chi Square, patient’s age was not statistically influencing the outcome in cardiac arrest patients (p = 0.507), but advanced response time was statistically influencing the outcome in cardiac arrest patients (p = 0.001). Conclusion: There was no statistically influence of patient’s age towards the outcome in cardiac arrest patients, but there was a significant influence of advance response time towards the outcome in cardiac arrest patients.
Correlation between ESBL-Producing Bacteria Infection with Sepsis Severity of Patient in Medical Ward of Internal Medicine Department Dr. Soetomo General Hospital in 2016
Authors: Pratiwi Asri Dhea , Rusli Musofa , Utomo Budi 
Published: September 2019

Introduction: Sepsis is life-threatening condition that begins with infections that trigger pro-inflammation overresponse. Delaying antibiotic treatment in sepsis can cause serious condition. In ESBL-producing bacteria, the antibiotic resistance is common and it might cause harm to sepsis patient. This research aims to analyze the correlation between infections of ESBL-producing bacteria with sepsis severity. Methods: This study was observational analytic on sepsis patient in Internal Medicine Ward of Dr. Soetomo General Hospital. The data were taken from medical records of the patient. The severity of sepsis was based on Surviving Sepsis Campaign 2012. Results: A total 72 sepsis patients were included in this study, which consist of 40 patients with non-producing ESBL GNB and 32 patients with ESBL-producing bacteria. The most common bacteria in ESBL infection is Escherichia coli ESBL+ (75%) and non ESBL infection is Acinetobacter baumanii (27.5%). Septic shock is more common in patient with ESBL-producing bacteria (53.1% vs 22.5%). The correlation between ESBL infection and sepsis severity is significant and the power of correlation is low (p = 0.048; r = 0.234). Conclusion: There is a correlation between ESBL-producing bacteria infection with sepsis severity and the power of correlation is low (p = 0.048, r = 0.234).
BIODIESEL SYNTHESIS OF COTTON SEEDS OIL (CEIBAPENTANDRA) BY CHEMICALLY TRANSESTERIFICATION PROCESS AND ION FRAGMENTATION OF METHYL ESTER
Authors: Gunawan Erin Ryantin , Wulandari Siska Ayu , Yuanita Emmy , Suhendra Dedy 
Published: September 2019

<p>Fossil fuel is commercial energy sources in many communities where their abundance progressively reduced and the resulting environmental pollution. Biodiesel is one alternative fuel made from vegetable which oils are suitable to replace the function of fossil fuels and environmentally friendly. Synthesis of biodiesel from cotton seeds oil was carried out by chemically ransesterification process. Composition of methyl esters in biodiesel thatwas analyzed by GC-MS are methyl palmitate (16.71%), methyl 8,11-octadecadienoic (46.45 %) and methyl linoleate (4.21 %).The highest amount of biodiesel conversion isat ratio of oil and methanol (1:1) with the catalyst 1% (weight of oil). The produced biodiesel was 93.7 %. Chemical properties of the produced biodiesel meets the standard SNI 04-7182-2006 i.e. saponification value of 120.167 mg KOH / g, acid value of 0.28 mg KOH / g and iod value 55.84g iod/100g of sample.</p>
Determinants of First Line Antiretroviral Treatment Failure among Patients on Highly Active Antiretroviral Therapy in selected Public Hospitals Jimma, Southwest Ethiopia a Case Control Study
Authors: Asfaw Amrachu Bekele , Tekelehaimanot Aderajew Nigusie , Shewanew Tamirat , Gudina Esayas Kebede , Getnet Masrie , Amdisa Demuma , Lemma Tefera Belachew , Dadi Lelisa Sena 
Published: September 2019

Abstract
Background: Highly Active Antiretroviral Therapy (HAART) has substantially declined morbidity and mortality related to Human immunodeficiency virus/Acquired immunodeficiency syndrome (HIV/AIDS). Despite this fact, first-line ART failure has emerged as a growing concern. However, factors associated with first-line ART failure are not well empathized and studied. Hence, we aimed to identify the determinants of first-line ART failure among patients attending ART in Public Hospitals Jimma, Southwest Ethiopia.
Methods: A case-control study was conducted in March 2018 on a sample of 384(288 controls and 96 cases) adult people living with HIV/AIDS (PLWHA). Cases were HIV patients aged 15 years or older who were on first-line ART regimens with documented therapeutic failure. Controls were HIV patients aged 15 years or older who were on first-line ART regimen but without evidence of therapeutic failure. Data were extracted from electronic databases and supplemented by data collected through interviewer-administered questionnaires. Bivariate and multivariate logistic regression analyses were used. Adjusted odds ratios and 95% confidence intervals were used to report independently associated factors at P-value<0.05.
Results: In this study, higher odds of first-line ART failure was experinced among urban residents (AOR:2.2;95%CI: 1.1, 3.6), smokers (AOR:5.9; 95%CI:3.2, 10.8), Khat users (AOR:2.2; 95%CI:1.3,3.7), poor treatment adherents (AOR:2.2; 95%CI: 1.1,4.5), tuberculosis coinfection (AOR:3.9; 95%CI:2.2, 6.8), prior exposure to ART (AOR:3.8; 95%CI:1.7, 8.1), zidovudine based regimen (AOR:4.8; 95%CI: 2.5,9.0) and longer duration on ART more than 73 months (AOR:1.9; 95%CI:1.2, 3.3).
Conclusions: This study evidenced that being an urban resident, TB co-infection, poor treatment adherence, and zidovudine-based regiment were positively and independently associated with first-line ART failure. Thus, enhanced adherence counseling should be provided for those urban residents as well as substance users to improve adherence to ART; early screening and management of tuberculosis is highly recommended. Moreover, close follow up of adverse effects of ARVs must be strengthened.
Keywords : determinants, first-line ART failure, HIV/AIDS, Jimma Zone, Public hospitals
WITHDRAWN: De novo assembly and comparative transcriptome analysis reveal genes potentially involved in changes of terpenoids in wheat germplasm resource, that defend against the wheat aphid Sitobion avenae (Fabricius)
Authors: Zhao Lei , Liu Zhen , Zhan Yidi , Liu Yong 
Published: September 2019

Abstract
Background Terpenoid volatiles play an important role in direct and indirect plant defense responses against herbivores, including in gramineous crops such as transgenic rice and corn. The conventional varieties of wheat, an important gramineous cereal crop, lack aphid-resistant genes. It is therefore, necessary to seek aphid-resistant genes by screening for potential terpenoid synthase genes in wheat germplasm resources. Results The result showed that aphid-damaged Octoploid Tirtitrigia emitted a higher amount of S-linalool, ent-kaurene, (+)-delta-cadinene, (3S,6E)- nerolidol compared to intact plant. In addition, (E)-β-caryophyllene, β‐Myrcene, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) were new volatile terpenoids emitted by the damaged plant. Further olfactory responses tests showed that S-linalool significantly repelled Sitobion avenae (Fabricius). Using the Illumina sequencing platform, approximately 203.09 million high quality paired-end reads were obtained. After de novo assembly and quantitative assessment, a total of 182,348(74.8%) unigenes were annotated by alignment with public protein databases. Of these unigenes, 2,389 differentially expressed genes were identified between intact and damaged ears of Octoploid Trititrigia. The expression profile of 10 randomly selected TPSs was confirmed with RT-qPCR. Candidate genes involved in terpenes biosynthesis were identified showing significant transcript changes between intact and damaged plant ears of Octoploid Trititrigia. Also transcript abundances of terpenes biosynthetic pathway-related genes were positively correlated with the production of volatile terpenoids in ears. The unigenes of S-linalool synthase gene was mapped to the cloned cDNA WT008_M07 (AK333728) and WT013_P07 (AK335856) of the Chinese spring wheat cultivar. The predicted protein complete ORF sequence (TaLIS1/2) when compared with the S-linalool synthase gene of other species, contained an aspartate-rich region DDxxD motif. Its function was characterized as coordinating the divalent metal ions involved in substrate binding. Furthermore, phylogenetic tree results showed that the TaLIS1 and TuNES1 are highly homologous. Conclusions This assembled transcriptome of S. avenae-damaged Octoploid Trititrigia and intact ears can provide more molecular resources for future functional characterization analysis of genomics in volatile terpenoids involved in direct or indirect defenses. Our study describes the metabolic regulation mechanism of volatile terpenoids in gramineous crops, which provides support for both breeding and genetic modification of wheat varieties resistant to wheat aphids.
Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars
Authors: Ding Haisheng , Liu Min , Zhou Changfan , You Xiangbin , Su Tao , Yang Youbing , Xu Dequan 
Published: September 2019

Abstract
Background: MicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis. Results: In this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270 days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-423-5p, ssc-mir-34c, ssc-mir-107, ssc-165 mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 ( PLCβ1) gene was verified to be a target of ssc-mir-423-5p . Conclusions: This study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars.
Integrated Analysis of lncRNA, miRNA and mRNA Reveals Novel Insights into the Fertility Regulation of Large White Sows
Authors: Hu Huiyan , Jia Qing , Xi Jianzhong , Zhou Bo , Li Zhiqiang 
Published: September 2019

Abstract
Background: Improving sow fertility is extremely important as it can lead to increased reproductive efficiency and thus profitability for swine producers. There are considerable differences in fertility rates among individual animals, but the underlying molecular mechanisms remain unclear. In this study, by using different types of RNA libraries, we investigated the complete transcriptome of ovarian tissue during the luteal (L) and follicular phases (F) of the estrous cycle in Large White pigs with high (H) and low fecundity (L), and performed a comprehensive analysis of long noncoding RNAs (lncRNAs), mRNAs and micro RNAs (miRNAs) from 16 samples by combining RNA sequencing (RNA-seq) with bioinformatics. Results: In total, 24,447 lncRNAs, 27,370 mRNAs, and 216 known miRNAs were identified in ovarian tissues. The genomic features of lncRNAs, such as length distribution and number of exons, were further analyzed. We selected a threshold of P < 0.05 and |log2 (fold change)| ≥ 1to obtain the differentially expressed lncRNAs, miRNAs and mRNAs by pairwise comparison (LH vs. LL, FH vs. FL). Bioinformatics analysis of these differentially expressed RNAs revealed multiple significantly enriched pathways (P < 0.05) that were closely involved in the reproductive process, such as ovarian steroidogenesis, lysosome, steroid biosynthesis, and the estrogen and GnRH signaling pathways. Moreover, bioinformatics screening of differentially expressed miRNAs that share common miRNA response elements (MREs) with lncRNAs and their downstream mRNA targets were performed. Finally, we constructed lncRNA–miRNA–mRNA regulation networks. The key genes in these networks were verified by Reverse Transcription Real-time Quantitative PCR (RT-qRCR), which were consistent with the results from RNA-Seq data.Conclusions: These results provide further insights into the fertility of pigs and can contribute to further experimental investigation of the functions of these genes.
“Little things do not get to me anymore”: a qualitative study of older adults’ perspectives on initiating exercise and mindfulness practice
Authors: Parra Diana C. , Wetherell Julie Loebach , Zandt Alexandria Van , Brownson Ross , Abhishek Janardan , Lenze Eric 
Published: September 2019

Abstract
Background: Mindfulness practice and exercise are ways by which older adults can improve and maintain their physical, emotional and cognitive health. Methods: This single-site qualitative study gathered insights of older adults’ perceptions about initiating and maintaining mindfulness and exercise practices. We carried out focus groups with 41 adults aged 65-85 who had recently initiated Mindfulness Based Stress Reduction (MBSR), structured exercise, or their combination as part of participation in a clinical trial. We used a semi-structured interview to ask them open-ended questions regarding the benefits, barriers and facilitators of participating in mindfulness and/or exercise interventions. The interview also included questions regarding translation of these practices into community settings as well as the long-term maintenance potential of these practices. Results : Older adults indicated that the mindfulness training increased their awareness and self-reflection and fostered a more self-accepting attitude. Furthermore, they improved their self-care habits and reported having better familial and social relationships. The main barrier for both the exercise and mindfulness group was time management. The social benefits and sense of community were some of the primary motivators for older adults in the exercise and/or MBSR interventions. However, the research on how to motivate older adults to initiate healthy behavioral changes also needs to be answered. The benefits of exercise and MBSR are a motivation in and of themselves, as indicated by some of the participants. Conclusions: This study indicates that mindfulness training and exercise can serve as tools to cultivate important health lifestyle qualities among older adults, who are in the midst of mental, social, emotional and physical change. If it were not for the purpose of the research or the incentives provided by the research team, these older adults may have never started the healthy behavioral changes. From the responses, this may indicate that older adults may need more incentives to begin and maintain behavioral changes other than for their own health benefit.
The DNA hypermethylation phenotype of colorectal cancer liver metastases resembles that of the primary colorectal cancers.
Authors: Orjuela Stephany , Menigatti Mirco , Schraml Peter , Kambakamba Patryk , Robinson Mark D. , Marra Giancarlo 
Published: September 2019

Abstract
Identifying molecular differences between primary and metastatic colorectal cancers—now possible with the aid of omics technologies—can improve our understanding of the biological mechanisms of cancer progression and facilitate the discovery of novel treatments for late-stage cancer. We compared the DNA methylomes of primary colorectal cancers (CRCs) and CRC metastases to the liver. Laser microdissection was used to obtain epithelial tissue (10 to 25 x 10 6 µm 2 ) from sections of fresh-frozen samples of primary CRCs (n=6), CRC liver metastases (n=12), and normal colon mucosa (n=3). DNA extracted from tissues was enriched for methylated sequences with a methylCpG binding domain (MBD) polypeptide-based protocol and subjected to deep sequencing. The performance of this protocol was compared with that of targeted enrichment for bisulfite sequencing used in a previous study of ours. MBD enrichment captured a total of 322,551 genomic regions (249.5 Mb of the genome) that included over seven million CpG sites. A few of these regions were differentially methylated at an expected false discovery rate (FDR) of 5% in neoplastic tissues (primaries: 0.67%, i.e., 2155 regions containing 279,441 CpG sites; liver metastases: 1%, i.e., 3223 regions containing 312,723 CpG sites) as compared with normal mucosa samples. Most of the differentially methylated regions (DMRs; 94% in primaries; 70% in metastases) were hyper methylated, and almost 80% of these (1882 of 2396) were present in both lesion types. At 5% FDR, no DMRs were detected in liver metastases vs. primary CRC. However, short regions of low-magnitude hypo methylation were frequent in metastases but rare in primaries. Hypermethylated DMRs were far more abundant in sequences classified as intragenic, gene-regulatory, or CpG shelves-shores-island segments, whereas hypomethylated DMRs were equally represented in extragenic (mainly, open-sea) and intragenic (mainly, gene bodies) sequences of the genome. Compared with targeted enrichment, MBD capture provided a better picture of the extension of CRC-associated DNA hypermethylation but was less powerful for identifying hypomethylation. Our findings demonstrate that the hypermethylation phenotype in CRC liver metastases remains similar to that of the primary tumor, whereas CRC-associated DNA hypomethylation probably undergoes further progression after the cancer cells have migrated to the liver.
The effect of periodontal bacteria infection on incidence and prognosis of cancer: a systematic review and meta-analysis
Authors: Xiao Li , Zhang Qianyu , Peng Yanshuang , Wang Daqing , Liu Ying 
Published: September 2019

Abstract
Background Periodontal bacteria is the major pathogens in the oral cavity and the main cause of adult chronic periodontitis, but their association with incidence and prognosis in cancer is controversial. The aim of this study was to evaluate the effect of periodontal bacteria infection on incidence and prognosis of cancer.Methods A systematic literature search of PubMed, Embase, Web of Science and Cochrane Library databases was performed to obtain 39 studies comprising 7184 participants. The incidence of cancer was evaluated as odd ratios (OR) with a 95% confidence interval (95% CI) using Review Manager 5.2 software. Overall survival (OS), cancer-specific survival (CSS) and disease-free survival (DFS), which were measured as hazard ratios (HR) with a 95% confidence interval (95% CI) using Review Manager 5.2 software.Results Our results indicated that periodontal bacteria infection increased the incidence of cancer (OR=1.25; 95%CI: 1.03–1.52) and was associated with poor OS (HR = 1.75; 95% CI: 1.40–2.20), DFS (HR=2.18; 95%CI: 1.24–3.84) and CSS (HR=1.85, 95%CI: 1.44–2.39). Subgroup analysis indicted that the risk of cancer was associated with Porphyromonas gingivalis (Pg) infection (OR=2.16; 95%CI: 1.34–3.47) and Prevotella intermedia (Pi) infection (OR=1.28; 95%CI: 1.01–1.63) but not Tannerella forsythia (Tf) (OR=1.06; 95%CI: 0.8–1.41), Treponema denticola (Td) (OR=1.30; 95%CI: 0.99–1.72), Aggregatibacter actinomycetemcomitans (Aa) (OR=1.00; 95%CI: 0.48–2.08) and Fusobacterium nucleatum (Fn) (OR=0.61; 95%CI: 0.32–1.16).Conclusion This meta-analysis revealed periodontal bacteria infection increased the incidence of cancer and predicted poor prognosis of cancer.