bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of a Vivid Homolog in Botrytis cinerea
Authors: Foley Brandon J. , Stutts Haley , Schmitt Sydney L. , Lokhandwala Jameela , Nagar Aditi , Zoltowski Brian D. 
Published: April 2018

AbstractBlue light‐signaling pathways regulated by members of the light–oxygen–voltage (LOV) domain family integrate stress responses, circadian rhythms and pathogenesis in fungi. The canonical signaling mechanism involves two LOV‐containing proteins that maintain homology to Neurospora crassa Vivid (NcVVD) and White Collar 1 (NcWC1). These proteins engage in homo‐ and heterodimerization events that modulate gene transcription in response to light. Here, we clone and characterize the VVD homolog in Botrytis cinerea (BcVVD). BcVVD retains divergent photocycle kinetics and is incapable of LOV mediated homodimerization, indicating modification of the classical hetero/homodimerization mechanism of photoadaptation in fungi.
Relationship between the flavonoid composition and flower colour variation in Victoria
Authors: Wu Q. , Li P.‐C. , Zhang H.‐J. , Feng C.‐Y. , Li S.‐S. , Yin D.‐D. , Tian J. , Xu W.‐Z. , Wang L.‐S. 
Published: April 2018

Abstract

Victoria (Nymphaeaceae), an annual or perennial aquatic plant genus, contains only two species: V. amazonica (Poepp.) J. C. Sowerby and V. cruziana A. D. Orb. Both species have large floating leaves and variable flower colour. Both Victoria species are night bloomers, which have white petals on the first blooming night that then turn pink or ruby red on the second blooming day. The mechanism of the colour change of Victoria petals during anthesis is still unclear.

In this study, flavonoids in Victoria petals of both species were evaluated and quantified by high‐performance liquid chromatography with photodiode array detection (HPLC‐DAD) and by ultra‐performance liquid chromatography coupled with tandem mass spectrometry (UPLC‐MS/MS) for the first time.

In total, 14 flavonoids were detected in Victoria petals, including 4 anthocyanins and 10 flavonols. The flavonoid compositions differed across the two species, resulting in different colours between the inner and outer petals. With increased anthocyanin content across blooming days, the colour of Victoria flowers changed over time.

The results of this study will improve understanding of the chemical mechanism of colour formation and lay the foundation for selective colour breeding in Victoria.
Outcomes of cryptococcosis in renal transplant recipients in a less‐resourced health care system
Authors: Ponzio Vinicius , Camargo Luis Fernando , Medina‐Pestana José , Perfect John Robert , Colombo Arnaldo Lopes 
Published: April 2018

AbstractBackgroundCryptococcosis is the second most common cause of invasive fungal infections in renal transplant recipients in many countries, and data on graft outcome after treatment for this infection is lacking in less‐resourced health care settings.MethodsData from 47 renal transplant recipients were retrospectively collected at a single institution during a period of 13 years. Graft dysfunction, graft loss, and mortality rates were evaluated. Predictors of mortality and graft loss were estimated.ResultsA total of 38 (97.4%) patients treated with amphotericin B deoxycholate (AMBd) showed graft dysfunction after antifungal initiation and 8 (18.2%) had kidney graft loss. Graft loss within 30 days after cryptococcosis onset was significantly associated with disseminated infection, greater baseline creatinine levels, and graft dysfunction concomitant to AMBd therapy and an additional nephrotoxic condition. The 30‐day mortality rate was 19.2% and it was significantly associated with disseminated and pulmonary infections, somnolence at admission, high CSF opening pressure, positive CSF India ink, creatinine levels greater than 2.0 mg/dL at admission, graft dysfunction in patients treated with AMBd and an additional nephrotoxic condition and graft loss within 30 days.ConclusionGraft dysfunction was common in renal transplant recipients with cryptococcosis treated with AMBd. The rate of graft loss rate was high, most frequently in patients with concomitant nephrotoxic conditions. Therefore, the clinical focus should be on the use of less nephrotoxic lipid formulations of amphotericin B in this specific population requiring a polyene induction regimen for treatment of severe cryptococcosis in all health care systems caring for transplantation recipients.
Improving inpatient warfarin therapy safety using a pharmacist-managed protocol
Authors: Daniels Paul R , Manning Dennis M , Moriarty James P , Bingener-Casey Juliane , Ou Narith N , O’Meara John G , Roellinger Daniel L , Naessens James M 
Published: April 2018

IntroductionSafe management of warfarin in the inpatient setting can be challenging. At the Mayo Clinic hospitals in Rochester, Minnesota, we set out to improve the safety of warfarin management among surgical and non-surgical inpatients.MethodsA multidisciplinary team designed a pharmacist-managed warfarin protocol (PMWP) which designated warfarin dosing to inpatient pharmacists with guidance from computerised dosing algorithms. Ordering this protocol was ultimately designed as an ‘opt out’ practice. The primary improvement measure was frequency of international normalised ratio (INR) greater than 5; secondary measures included adoption rate of the protocol, a counterbalance INR metric (INR <1.7 three days after first inpatient warfarin dose), and complication rates, including bleeding and thrombosis events. An interrupted time series analysis was conducted to compare outcomes.ResultsAmong over 50 000 inpatient warfarin recipients, the PMWP was adopted for the majority of both surgical and non-surgical inpatients during the study period (1 January 2005 to 31 December 2011). The primary improvement measure decreased from 5.6% to 3.4% for medical patients and from 5.2% to 2.4% for surgical patients during the preimplementation and postimplementation periods, respectively. The INR counterbalance measure did not change. Postoperative bleeding decreased from 13.5% to 11.1% among surgical patients, but bleeding was unchanged among medical patients.ConclusionOur PMWP led to achievement of improved INR control for inpatient warfarin recipients and to less near-term bleeding among higher risk, surgical patients.
O realismo maravilhoso em o Sumiço da santa: uma história de feitiçaria, de Jorge Amado
Authors: Melo Valdirene Rosa da Silva , Brandão Saulo Cunha da Serpa 
Published: April 2018

O presente artigo tem como objetivo tecer conjecturas sobre o realismo maravilhoso presente na obra O Sumiço da santa: uma história de feitiçaria, do escritor baiano Jorge Amado. Considerando-se que na citada obra é possível perceber a recorrência de elementos sobrenaturalizados povoando o ambiente narrativo de maneira natural e amalgamados com a realidade será proposta para esta análise uma leitura crítico-interpretativa do Realismo Maravilhoso como um elemento de transgressão que ganha espaço dentro da narrativa.Tal norteamento permitirá dar à obra um olhar literário contestador e inovador, capaz de contrapor um modelo cultural moldado em padrões europeus à simplicidade da cultura local repleta de misticismo e crendices populares.
Burlesco, satírico e duplo: as faces de “O Duque de L’Omelette”
Authors: Gabriel Maria Alice Ribeiro , Santos Luciane Alves 
Published: April 2018

“O Duque de L’Omelette” é um conto do escritor Edgar Allan Poe publicado em 1832 pelo periódico Philadelphia Saturday Courier. Nesta obra satírica, Poe elege o motivo gótico do pacto com o diabo, mas seu sarcasmo tem alusões contextuais específicas e, portanto, a crítica considerou o relato menos importante em si mesmo. O objetivo deste estudo é definir o duplo como um tema do gênero Fantástico que possivelmente segue presente nesta narrativa burlesca de Poe.
Initial Right Ventricular Dysfunction Severity Identifies Severe Peripartum Cardiomyopathy Phenotype With Worse Early and Overall Outcomes: A 24‐Year Cohort Study
Authors: Peters Andrew , Caroline Mara , Zhao Huaqing , Baldwin Matthew R. , Forfia Paul R. , Tsai Emily J. 
Published: April 2018

Background

Outcomes in peripartum cardiomyopathy (
PPCM
) vary. We sought to determine whether severity of left or right ventricular dysfunction (RVD) at
PPCM
diagnosis differentially associates with adverse outcomes.

Methods and Results

We conducted a single‐center retrospective cohort study of 53 patients with PPCM. The primary outcome was a composite of left ventricular assist device implantation, cardiac transplantation, or death. We used Kaplan‐Meier curves to examine event‐free survival and Cox proportional hazards models to examine associations of left ventricular (LV) ejection fraction <30%, LV end‐diastolic diameter ≥60 mm, and moderate‐to‐severe
RVD
at
PPCM
diagnosis with the primary outcome. Median (
interquartile range
) follow‐up time was 3.6 (1.4–7.3) years. Seventeen patients (32%) experienced the primary outcome, of whom 11 had moderate‐to‐severe
RVD
at time of
PPCM
diagnosis. Overall event‐free survival differed by initial
RVD
severity and
LV ejection fraction
<30%, but not by LV
end‐diastolic diameter
≥60 mm. In univariable analyses, LV ejection fraction <30% and moderate‐to‐severe
RVD
were associated with the outcome (hazard ratios [95%
confidence intervals]
of 4.85 [1.11–21.3] and 4.26 [1.47–11.6], respectively). In a multivariable model with
LV ejection fraction
<30%,
LV end‐diastolic diameter
≥60 mm, and moderate‐to‐severe
RVD
, only moderate‐to‐severe
RVD
was independently associated with the outcome (hazard ratio [95% confidence interval
]
, 3.21 [1.13–9.10]). Although most outcomes occurred within the first year, nearly a third occurred years after
PPCM
diagnosis.

Conclusions

Initial moderate‐to‐severe
RVD
is associated with a more advanced cardiomyopathy phenotype and increased risk of adverse outcomes in
PPCM
, within and beyond the first year of diagnosis. By identifying a worse
PPCM
phenotype, initial moderate‐to‐severe
RVD
may prompt earlier consideration of advanced heart replacement therapies.
Beyond Abaclat: Mass Claims in Investment Treaty Arbitration and Regulatory Governance for Sovereign Debt Restructuring
Authors: Nakajima Kei 
Published: April 2018

Abstract
Whereas investment treaties and arbitration rules do not usually provide any explicit provision for mass claims in investment treaty arbitration, the Tribunal in Abaclat v Argentina established a landmark jurisprudence that allowed a massive 60,000 investors to bundle and bring their claims before a single arbitral tribunal. However, its reasoning has been severely criticised for its conclusion, which apparently favours bondholder protection at the expense of financial policy leeway of defaulted sovereigns: investment arbitration may adversely affect the orderly implementation of sovereign debt restructuring. This article attempts to take a more balanced approach towards this issue, by focusing on regulatory aspects of arbitral proceedings. A ‘regulatory’ investment treaty arbitration will not only provide creditor protection by opening the door for mass claims, but will also show a deference to an orderly restructuring by closing the door if circumstances so require.
A new species of the genus Bryodemella (s. str.) Yin, 1982 from China (Orthoptera: Acridoidea: Oedipodidae)
Authors: ZHANG YU-LONG , ZHI YONG-CHAO , ZHANG DAO-CHUAN 
Published: April 2018

A new species (i.e. Bryodemella (s. str.) rufifemura sp. nov. from China is described in this paper. It is similar to Bryodemella (s. str.) diamesum (Bei-Bienko, 1930), but differs from the latter by red inner side of hind femur, median keel of pronotum indistinct in metazoan, and vertical diameter of eye shorter than subocular groove in female. The type specimens are deposited in the Museum of Hebei University (MHU), China.
The Impact of Recent Forcing and Ocean Heat Uptake Data on Estimates of Climate Sensitivity
Authors: Lewis Nicholas , Curry Judith 
Published: April 2018

AbstractEnergy budget estimates of equilibrium climate sensitivity (ECS) and transient climate response (TCR) are derived based on the best estimates and uncertainty ranges for forcing provided in the IPCC Fifth Assessment Report (AR5). Recent revisions to greenhouse gas forcing and post-1990 ozone and aerosol forcing estimates are incorporated and the forcing data extended from 2011 to 2016. Reflecting recent evidence against strong aerosol forcing, its AR5 uncertainty lower bound is increased slightly. Using an 1869–82 base period and a 2007–16 final period, which are well matched for volcanic activity and influence from internal variability, medians are derived for ECS of 1.50 K (5%–95% range: 1.05–2.45 K) and for TCR of 1.20 K (5%–95% range: 0.9–1.7 K). These estimates both have much lower upper bounds than those from a predecessor study using AR5 data ending in 2011. Using infilled, globally complete temperature data give slightly higher estimates: a median of 1.66 K for ECS (5%–95% range: 1.15–2.7 K) and 1.33 K for TCR (5%–95% range: 1.0–1.9 K). These ECS estimates reflect climate feedbacks over the historical period, assumed to be time invariant. Allowing for possible time-varying climate feedbacks increases the median ECS estimate to 1.76 K (5%–95% range: 1.2–3.1 K), using infilled temperature data. Possible biases from non–unit forcing efficacy, temperature estimation issues, and variability in sea surface temperature change patterns are examined and found to be minor when using globally complete temperature data. These results imply that high ECS and TCR values derived from a majority of CMIP5 climate models are inconsistent with observed warming during the historical period.
Evaluation of the Prognostic Accuracy of Astler-coller's and Jass’ Classifications of Colorectal Cancer
Authors: Li Destri Giovanni , Rinzivillo Calogero , Vasquez Enrico , Di Cataldo Antonio , Puleo Stefano , Licata Antonio 
Published: April 2018

Aim The study aim was to determine whether the Jass classification is superior to that of Astler-Coller (modification of Dukes’ stage) in determining prognosis of patients treated for colorectal carcinoma. Study Design The authors used Jass’ classification to restage 263 patients who had undergone radical colorectal surgery and classified according to Astler-Coller. Results The results revealed that: 1) Astler-Coller's classification enables more accurate selection of patient groups where life expectancy can be predicted; 2) Jass’ classification enables statistically significant (P <0.05) improved prognostic discrimination of Astler-Coller's B2 patients, for which the probability of nonrecurrence of the disease is around 60%. Conclusions Although Astler-Coller's classification is still valid, Jass’ classification is useful for the prognostic discrimination of Astler-Coller's B2 patients.
Cadherins and Integrins in Renal Cell Carcinoma an Immunohistochemical Study
Authors: Markovic-Lipkovski Jasmina , Brasanac Dimitrije , Müller Gerhard A , Müller Claudia A 
Published: April 2018

Aims and Background The aim of this study was to determine the expression of cadherins and integrins in renal cell carcinoma (RCC) and their relationship with tumor morphology and TNM status. Methods Cadherin and integrin expression was investigated using an indirect immunoperoxidase technique, applying antibodies to E-, N-, P- and VE-cadherin and to α1, α2, α3, α4, α5, α6 and αv integrin subunits. Correlation of semiquantitatively scored adhesion molecule levels with histopathological parameters (cytology, growth pattern, nuclear grade) and TNM status was performed for 24 RCCs (17 clear cell, 3 granular, 3 spindle cell and 1 chromophobe cell type according to the WHO classification). Results E-cadherin and N-cadherin were present in most cases (88% and 67%, respectively) and were usually coex-pressed. T3 RCCs displayed higher E-cadherin and N-cadherin levels than T1/T2 tumors regardless of tumor grade, suggesting that impairment of their function might exist without actual loss from tumor cells. P-cadherin was found focally in two RCCs only, while VE-cadherin was present on stromal vessel endothelium in five tumors, showing no differences with regard to cell type, growth pattern, tumor grade or TNM status. All integrins were present in the studied RCCs (ranging from 12% for α5 to 79% for α3), including those that are normally absent from adult kidney tissue (α4 and α5). Tumors of higher grade showed increased αv and decreased α6 levels, while RCCs with metastases less often showed diffuse α3 presence and never expressed α5 integrin. Conclusions Our results suggest that the level of expression of N-cadherin and some integrins (most notably α3, α6, and α5) is associated with the capacity of RCC for local and distant spread, regardless of tumor grade.
Deep Venous Thrombosis of the Extremity Diagnosed by Color Doppler Ultrasonography after Isolated Limb Perfusion
Authors: Eroğrlu Aydan , Özcan Hasan , Eryavuz Yavuz , Kocaoğlu Hilmi , Demirci Salim , Aytac Suat K 
Published: April 2018

Aim Isolated limb perfusion (ILP) is used to treat locally advanced sarcoma and melanoma of the extremities. ILP is associated with a 1.7% to 10% incidence of deep venous thrombosis (DVT). The aim of the study was to examine the effect of ILP on the peripheral venous system and to evaluate the diagnostic accuracy of color Doppler ultrasonography (US). Methods A total of 26 patients with locally advanced sarcoma or melanoma of the extremities received a total of 38 ILP. The patients were evaluated preoperatively and postoperatively by color Doppler US as a noninvasive venous measurement. We used a color Doppler US system (SSA-270A, Toshiba) with a 7.5-MHz linear transducer for gray-scale imaging and a 5-MHz vascular transducer for color Doppler imaging. Results Seventeen patients underwent single ILP while the others were treated with multiple ILPs. Color Doppler US showed a reflux flow in three (7.9%) patients and DVT occurred subsequently in these patients. Conclusions Color Doppler US is a noninvasive and clinically useful diagnostic technique in the diagnosis of extremity DVT. We recommend anticoagulant therapy in patients with reflux on ultrasonographic examination.
High-grade Cervical Squamous Intraepithelial Lesion during Pregnancy
Authors: Candido Murta Eddie Fernando , De Souza Flávio Henrique Caetano , De Souza Maria Azniv Hazarabedian , Adad Sheila Jorge 
Published: April 2018

Aims and Background An increasing incidence of high-grade squamous intraepithelial lesion (HSIL) has been observed among young women. Consequently, an increased number of cases are being discovered during pregnancy. We analyzed the clinical and therapeutic management of HSIL during pregnancy. Methods A retrospective study was conducted from 1979 to 1998, and 58 registries of women with a cytological or histological diagnosis of HSIL during the pregnant-puerperal period were reviewed. Information obtained from medical records included age, gestational age at diagnosis, parity, age of first intercourse, number of sexual partners before pregnancy, tobacco use, cytologic and colposcopic findings, route of delivery, postpartum follow-up, and treatment. This information was compared with a non-pregnant control group with HSIL. Results The average age of pregnant women with HSIL was 27.9 ± 5.2 years. The cytologic or histologic diagnosis of HSIL was made in the first trimester in 12 (20.7%) women and in the second trimester in 30 (51.7%) women. Average parity was 2.8 ± 2 deliveries. Age of first intercourse ranged from 13 to 29 years (16.1 ± 3.3). Thirty-two women (55.1%) had more than one sexual partner before pregnancy. Thirty-seven (63.8%) refereed tobacco use. According to the aforementioned aspects, no statistical difference was found in relation to control, except to cervical ectopia, which was more frequent in pregnant women (56.9% versus 42.6%). From the total of 58 pregnant women with cytologic or biopsy HSIL diagnosis, 53 had HSIL diagnosis made on cervical biopsy directed by colposcopy performed during the pregnancy; 44 (83%) of them were submitted to conservative management. HSIL was diagnosed by cervical biopsy in postpartum evaluation in 76% pregnant women with vaginal delivery and 78.6% women who underwent cesarean section. Conclusions A conservative management of HSIL in pregnancy is proposed, with colposcopic evaluation during gestation and postpartum, regardless of route of delivery.
Reactivation of Pulmonary Tuberculosis in Malignancy
Authors: Karnak Demet , Kayacan Oya , Beder Sumru 
Published: April 2018

Aims and Background Deterioration of immunity due to local or systemic effects of the tumor itself and/or administered chemotherapeutics or radiotherapy may play roles in the reactivation of tuberculosis, increasing the mortality in patients with various malignancies. In a country with a high prevalence of tuberculosis such as Turkey, most people have inactive tuberculous lesions and tuberculin test positivity. Therefore, a prospective study was carried out to investigate the frequency of tuberculosis reactivation in patients with a malignancy. Methods Seventy-three patients with a malignancy and undergoing diagnostic fiberoptic bronchoscopy were enrolled in the study during a 2-year period (1993-1995). Bronchoscopic biopsies and cytologic materials were obtained. Bronchoalveolar lavage fluids, bronchial washings, and pre- and post-bronchoscopic sputum specimens were also evaluated for acid-fast bacilli. A diagnosis of tuberculosis was based on smear and/or culture positivity for acid-fast bacilli. Results The mean age of the patients was 56.2 ± 13.6 years, with a male/female ratio of 69/4. The biopsy proven malignancies were as follows: primary lung carcinoma (n = 66, 90.4%), lymphoma (n = 5, 6.8%), metastatic breast adenocarcinoma (n = 1, 1.4%), and acute myelocytic leukemia (n = 1, 1.4%). Thirty-one of all patients had findings compatible with tuberculosis on radiology. The sputa and bronchial washing specimens were smear negative in all patients. Acid-fast bacilli were grown on culture in 6 patients (8%) (primary lung cancer, n = 5; non-Hodgkin lymphoma, n = 1). Four of these 6 patients had positive radiology for tuberculosis. These subjects were treated with a three- or four-drug anti-tuberculosis regimen. Two months later, smears remained acid-fast bacilli negative, or no bacilli were grown on culture. Conclusions The possibility of coexisting tuberculosis should be kept in mind in patients with a malignancy, especially those with lung carcinoma in countries with a high prevalence of tuberculosis. Pulmonary infections encountered in such patients should raise the suspicion of tuberculosis reactivation, and in addition to direct microscopic evaluation, sputum specimens and materials obtained by fiberoptic bronchoscopy should be cultivated for tuberculosis. Three-four-drug anti-tuberculosis regimens should be given, especially in countries with high drug-resistance rates for eradicating tuberculosis.