bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of bacteria colonizing the mucosal layer of the gastrointestinal tract of Atlantic salmon farmed in a warm water region
Authors: Hall Kate , Reid Chantelle E , Hamlett Josephine , Kaur Damanpreet , Tan Qi Zhi , Taylor Richard S , Bissett Andrew , Nowak Barbara F , Bowman John P 
Published: November 2024

AbstractAtlantic salmon (Salmo salar) farmed in seawater in Tasmania (lutruwita) can experience temperatures close to their thermotolerance limit during summer. Gut microbiome data from eight successive annual surveys and a specific survey of GI tract mucosa and digesta bacterial cross-sectional distributions indicated members of the genusAliivibrio,Vibrio, and an unclassified Mycoplasmoidaceae are the main colonizers of the gut mucosal layer in Tasmanian farmed salmon. Peak abundance levels are reached after 7-8 months, corresponding to the late summer, following the transfer of hatchery smolt to seawater farms. SalmonAliivibrioisolates comprise three novel non-bioluminescent species distinct from species found in the northern hemisphere growing regions. Along with otherAliivibriospecies these species share many genes required for host colonization and biofilm formation and also include species and strain-level dependent features. Two of the novelAliivibriospecies surprisingly possessed genes for cytolethal distending toxin while the more predominant species lacked any known virulence genes. The overall observations suggest a small number of species actively colonize the mucosal layer of Atlantic salmon farmed in Tasmania and that this process is strongly influenced by the environmental temperature.
Assessing RNA velocity stability across synthetic replicates using count splitting
Authors: Li Yuhong , Wei Zeyu Jerry , Chen Yen-Chi , Lin Kevin Z. 
Published: November 2024

AbstractRNA velocity is a computational framework that enables predicting future cell states based on single-cell RNA sequencing data and offers valuable insights into dynamic biological processes. However, there is a lack of general methods to quantify the uncertainty and stability of these predictions from various RNA velocity methods. In this paper, we present a novel framework for evaluating the stability of RNA velocity estimates by generating two independent splits of the original data using negative binomial count splitting. We apply our workflow to five RNA velocity methods and two well-characterized datasets about mouse erythroid development and pancreatic endocrinogenesis. Our results highlight key differences between methods, identify inconsistencies such as reversed velocity flow between different RNA velocity methods, and demonstrate robustness when certain intermediary cell states are missing. Our framework is broadly applicable across RNA velocity methods and provides a robust way to assess and compare method performance. This generalizability makes our approach an important tool for selecting reliable RNA velocity methods in diverse biological contexts.
Fisetin extends lifespan in a murine model of recessive dystrophic epidermolysis bullosa
Authors: Miller William C. , Strege Chloe L. , Popp Courtney M. , Tito Simone M. Intriago , Eide Cindy , Ebens Christine L. , Riddle Megan , Lees Chris , Seelig Davis , Bui Kacey , Yousefzadeh Matthew J , McGrath John , Tolar Jakub 
Published: November 2024

AbstractRecessive Dystrophic Epidermolysis Bullosa (RDEB) is a rare genodermatosis characterized clinically by extensive inflammation, cutaneous destruction, and fibrosis that demonstrates properties similar to rapid skin aging. As tissue ages, it accumulates cellular damage and exhaustion leading to a state of senescence. Cellular senescence is an aging or disease-related phenomenon of stable exit from the cell cycle that leads to an increased inflammatory phenotype. RDEB and other EB subsets of patients need an adjunct to or alternate therapy that addresses the issues of inflammation, pain, and pruritus. Fisetin is a safe, naturally occurring compound proven to be effective at sensitizing senescent cells to cell death and ameliorating senescence-associated inflammation. In this paper, we demonstrate fisetin’s ability to increase survival and reduce senescent cell burden in a hypomorphic mouse model of RDEB.
The conserved ComP structure with two distinct DNA binding modes - systematic modeling with AlphaFold3, RoseTTAFold2NA, Chai-1 and re-docking in HADDOCK
Authors: Helsem Stian Aleksander , Alfsnes Kristian , Frye Stephan A. , Hesselberg Løvestad Alexander , Ambur Ole Herman 
Published: November 2024

AbstractThe competence protein ComP is the extracellular DNA binding protein involved in the uptake of DNA inNeisseria gonorrhoeaeand related Neisserieaceae bacteria. Details of the DNA binding mode of ComP remains enigmatic. Here we identify the ComP orthologs in a set of Neisseriaceae family members, model their common structural domains and their interaction with different preferred binding motifs, DNA Uptake Sequences (DUS). Through systematic in-silico modeling and comparisons using AlphaFold 3, RoseTTAFold2NA, and Chai-1, we bring a new understanding of the interactions between DNA and ComP, and particularly between DUS and ComP. We report six distinct binding modes of which two, here named Epsilon and Gamma, were repeatedly top-scoring models across platforms and different ComPs. The characteristics and robustness of the predicted models from each tool are assessed and discussed. This work may further expand the knowledge on the ComP-DUS interaction and guide further systematic and experimental characterization of these complexes which form extracellularly.
Horizontal transfer of a LINE-RTE retrotransposon among parasite, host, prey and environment
Authors: Brann T. , de Oliveira F. S. , Protasio A. V. 
Published: November 2024

AbstractBackgroundHorizontal transfer of transposable elements is both impactful, owing to the subsequent transposition burst, and insightful, providing information on organisms’ evolutionary history. In eukaryotes, horizontal gene transfer (HGT) often involves transposable elements (TEs), host-parasite relationships, aquatic environments or any of them combined. The flatwormSchistosoma mansoniis a human parasite with two free-living aquatic stages (intercalated between a definitive human host and intermediate snail host) and has a sizable TE content. We aimed to identify and characterise potential instances of HGT leveraging new genomic resources available.ResultsUsing the latest chromosome-scale genome assembly and available TE sequences we identify two putatively horizontally transferred elements, named Perere-3 and Sr3, in theS. mansonigenome. We demonstrate the presence of these TEs in the genomes ofSchistosomaspp. intermediate hosts, most likely explained by HGT. Perere-3 / Sr3 were also found across a wide range of additional organisms not susceptible to schistosome infection, including turtles, fish and other molluscs.ConclusionsWe propose that the patchy distribution of Perere-3/Sr3 across the phylogenetic tree is best explained by HGT. This phenomenon is likely linked to the parasitic nature of schistosomes, as several snail species sharing the elements are susceptible to infection. However, presence of Perere-3/Sr3 in species beyond this relationship may suggest wider ancestralSchistosomatidaehost ranges and/or undescribed schistosomes.
Flexible Learning and Re-ordering of Context-dependent Object Sequences in Nonhuman Primates
Authors: Wen Xuan , Neumann Adam , Dhungana Seema , Womelsdorf Thilo 
Published: November 2024

AbstractIntelligent behavior involves mentally arranging learned information in novel ways and is particularly well developed in humans. While nonhuman primates (NHP) will learn to arrange new items in complex serial order and re-arrange neighboring items within that order, it has remained contentious whether they are capable to re-assign items more flexibly to non-adjacent positions. Such mental re-indexing is facilitated by inferring the latent temporal structure of experiences as opposed to learning serial chains of item-item associations. Here, we tested the ability for flexible mental re-indexing in rhesus macaques. Subjects learned to serially order five objects. A change of the background context indicated when the object order changed, probing the subjects to mentally re-arrange objects to non-adjacent positions of the learned serial structure. Subjects successfully used the context cue to pro-actively re-index items to new, non-adjacent positions. Mental re-indexing was more likely when the initial order had been learned at a higher level, improved with more experience of the re-indexing rule and correlated with working memory performance in a delayed match-to-sample task. These findings suggest that NHPs inferred the latent serial structure of experiences beyond a chaining of item-item associations and mentally re-arrange items within that structure. The pattern of results indicates that NHPs form non-spatial cognitive maps of their experiences, which is a hallmark for flexible mental operations in many serially ordered behaviors including communication, counting or foraging.
Antibiotic treatment and microbiome depletion slow cnidarian regeneration
Authors: Da-Anoy Jeric , Toyama Kyle , Jasnos Oliwia , Wong Audrey , Gilmore Thomas D. , Davies Sarah W. 
Published: November 2024

AbstractCnidarian microbiomes play an essential role in physiology and development, but how these communities influence tissue regeneration is poorly understood. Here, we examined the effects of antibiotic exposure on regeneration and microbial communities in two cnidarian models, the sea anemonesNematostella vectensis(non-symbiotic, hereafter,Nematostella) andExaiptasia pallida(symbiotic, hereafter,Aiptasia). Bisected animals were incubated in either sterile or antibiotic-containing artificial seawater for seven days and regeneration was monitored daily. After seven days, tentacle number and length were measured, and microbial communities were profiled using metabarcoding of the V4 region of the 16S rRNA. Microbiome disruption was observed under antibiotic treatment in both species, resulting in decreased microbial load and shifts in relative abundances of some microbial taxa. However,Nematostellaexhibited a greater reduction in microbial diversity and community shifts under antibiotic exposure, whereasAiptasiashowed only moderate changes in diversity. In both species, microbiome disruption was associated with slower regeneration rates and reduced tentacle number and length, suggesting a functional role for the microbiome in cnidarian regeneration. Our findings suggest that host-microbiome interactions in both symbiotic and aposymbiotic anemones are important for the maintenance of regenerative processes. These findings provide insight into how cnidarians and their microbiomes respond to environmental stressors, with implications for predicting cnidarian resilience in the context of emerging threats to the marine environment.
Controlling Reciprocity in Binary and Weighted Networks: A Novel Density-Conserving Approach
Authors: Hadaeghi Fatemeh , Fakhar Kayson , Hilgetag Claus C. 
Published: November 2024

AbstractWe introduce efficient Network Reciprocity Control (NRC) algorithms for steering the degree of asymmetry and reciprocity in binary and weighted networks while preserving fundamental network properties. Our methods maintain edge density in binary networks and cumulative edge weight in weighted graphs. We test these algorithms on synthetic benchmark networks—including random, small-world, and modular structures— as well as brain connectivity maps (connectomes) from various species. We demonstrate how adjusting the asymmetry-reciprocity balance under edge density and total weight constraints influences key network features, including spectral properties, degree distributions, community structure, clustering, and path lengths. Additionally, we present a case study on the computational implications of graded reciprocity by solving a memory task within the reservoir computing framework. Furthermore, we establish the scalability of the NRC algorithms by applying them to networks of increasing size. These approaches enable systematic investigation of the relationship between directional asymmetry and network topology, with potential applications in computational and network sciences, social network analysis, and other fields studying complex network systems where the directionality of connections is essential.
Magnetization-current-induced
E1
transition in the
d+t→α+n+γ
reaction
Authors: Timofeyuk N. K. 
Published: November 2024

The d−t collisions have a minor reaction branch, of the order of 10−5, involving γ emission from the incident state with the spin of 3/2+ to the final 3/2− and 1/2− states of the α−n system. Standard E1 treatment of such a transition results in zero cross sections because of the spin conservation rule. For this reason, the first theoretical work on d+t→α+n+γ [], described the E1 transition as coming from the high-energy d-wave spin-1/2 state of the α−n partition of He5, coupled to the d−t component by tensor interaction. However, the exact E1 operator contains a contribution from the usually neglected magnetization current, represented by the nucleon spin operators, which can allow the E1 d+t→α+n+γ transition to occur without passing via the d-wave spin-1/2 α−n channel. The present paper studies the magnetization-current-induced E1 transition using several potential models of the d−t interaction. It was found that the strength of this transition depends strongly on the choice of the d−t interaction model. With the optical folding potential, correctly capturing the deuteron extended structure, this contribution is suppressed by three orders of magnitude, thus validating its neglect in the previous work.

Published by the American Physical Society
2024
How coaching project manager influence construction project team members’ job burnout: a conservation of resources perspective
Authors: Lu Yongqiang , Ma Li 
Published: November 2024

PurposeConstruction project team members’ job burnout damages the physical health of members and also have a negative effect on project performance. This study primarily aims to empirically examine the relationship between coaching project managers (CPMs) and team members’ job burnout. Moreover, this research examines the cross-level mediating effect of team caring ethical climate and team members’ team-based self-esteem (TBSE) on the relationship between the two aspects.Design/methodology/approachThis study uses conservation of resources theory as basis to construct a cross-level research model of the effect of CPMs on team members’ job burnout. Thereafter, regression analysis was performed on a sample of 431 team members from 83 teams.FindingsAccording to the empirical results, the authors found that, in construction project teams, first, CPMs were negatively correlated with team members’ job burnout and positively correlated with caring ethical climate and team members’ TBSE. Second, caring ethical climate and team members’ TBSE played a cross-level mediating role between CPMs and team members’ job burnout. In addition, caring ethical climate played a cross-level moderating role in the negative relationship between team members’ TBSE and job burnout.Originality/valueThis study introduces coaching leadership, an important leadership type, into the research background of construction project teams, thereby theoretically enriching the research on construction project team leadership. Moreover, by further expanding the research on the consequences of coaching leadership to the field of job burnout, this study also enriches the theoretical results of the research on the consequences of coaching leadership.
All is not well: value co­destruction and consumer ill­being in service systems
Authors: Gummerus Johanna Katariina , O’Loughlin Deirdre Mary , Kelleher Carol , von Koskull Catharina 
Published: November 2024

Purpose
Following an interpretivist approach, the authors draw on semi-structured interviews with parents of children with autism spectrum disorder (ASD).

Design/methodology/approach
Responding to limited academic advancement, particularly in the context of consumers experiencing vulnerability, the aim is to deepen marketing scholars’ understanding of value co-destruction (VCD) and its under-explored relationship with consumer ill-being.

Findings
Three forms of systemic VCD mechanisms emerged: obscuring, gaslighting and siloing. Ill-being comprised material, physical, psychological and social harms, which consumers experienced individually, relationally and collectively due to VCD. Family members’ experiences of ill-being and vulnerability were deepened by service systems’ inability to recognise the individuality of their needs and provide appropriate support.

Research limitations/implications
In line with the interpretivist paradigm, the focus on families of children with ASD, while illuminating, delimits the generalisability of the findings. The authors call for further research on consumer ill-being, VCD and vulnerability in other service and marketing contexts.

Practical implications
The findings highlight the need for service system adaptability to recognise and address unstandardised needs.

Social implications
Several systemic failures of (public) service systems which manifested as VCD mechanisms are identified.

Originality/value
The overall contribution is the development of a contextually driven characterisation of both VCD and ill-being and a deeper understanding of how these are interrelated. First, VCD revealed itself as a systemic failure to access, provide or integrate resources to meet actors’ needs as manifested by the three mechanisms. Second, the authors characterise ill-being as comprising material, physical, psychological and social harms due to VCD, which are experienced individually, relationally and collectively. Finally, the authors illuminate the nature of vulnerability and delineate the entanglements between vulnerability and ill-being in a collective (e.g. family) context.
Journey into meta-commerce: unveiling the driving forces of consumer adoption
Authors: Balhareth Hamad , Al-Adwan Ahmad Samed , Alsoud Anas Ratib , Abd-Aljaber Omar , Al-Rahmi Waleed Mugahed , Urbaníková Marta 
Published: November 2024

Purpose
This study aims to investigate the determinants of meta-commerce (metaverse commerce) adoption intention from the consumer perspective.

Design/methodology/approach
This research conducted a quantitative cross-sectional field survey to gather primary data from consumers in Jordan. The study aimed to analyze the adoption of meta-commerce from the consumer perspective. The survey collected responses from 574 participants, and the obtained data set was subjected to rigorous analysis using SmartPLS4 to test the proposed hypotheses.

Findings
While perceived herd behavior, consumer innovativeness, perceived ease of use, hedonic motivation and perceived usefulness positively influence consumers’ meta-commerce adoption intention, perceived cyber risks negatively influence it. Furthermore, perceived herd behavior and consumer innovativeness positively affect ease of use and usefulness perceptions.

Originality/value
This study presents a significant theoretical contribution by examining meta-commerce adoption from the consumer’s perspective, addressing a gap in the literature. To the best of the authors’ knowledge, this study is one of the first empirical investigations to explore meta-commerce adoption intentions from the consumer standpoint. Furthermore, it provides a novel extension of the “Technology Acceptance Model” for understanding meta-commerce adoption intention that captures the interplay between enablers (perceived ease of use and perceived usefulness), barriers (perceived cyber risks), intrinsic motivation (hedonic motivation), social factors (perceived herd behavior) and personal characteristics (consumer innovativeness), thereby extending previous research on the effects of these factors on the adoption of meta-commerce.
Financial anxiety of university students in Poland and Czechia: fsQCA analysis
Authors: Ahamed A.F.M. Jalal , Jakubowska Dominika , Sadílek Tomáš 
Published: November 2024

PurposeThis study aims to formulate propositions based on combinations of causal conditions that lead to high or low financial anxiety among European students, particularly in Poland and Czechia.Design/methodology/approachThe data for this study were collected in September 2023 from 265 undergraduate and graduate students with their income at one university in Poland and three in Czechia. Students’ views on financial anxiety were explored using a seven-item Likert scale. This study uses fuzzy set qualitative comparative analysis (fsQCA), an emerging marketing research technique.FindingsThere are specific factors that may cause increased financial anxiety among young adults in Poland and Czechia, leading to the following key findings: (1) A mix of factors such as perceived lack of financial knowledge, being female, living with parents, having a low monthly income, single status and working a few hours or not at all is linked to higher financial anxiety. (2) Experiencing financial anxiety is also likely when there is low financial knowledge, female gender, living away from parents, single status and a high number of work hours. (3) The combination of low financial literacy, female gender, living with parents, being single and working more hours can elevate financial anxiety.Originality/valueThis study expands the scope of personal financial research by examining how cultural, socioeconomic and psychological factors affect students’ financial anxiety in two European countries that were infrequently studied in this context. It contributes to identifying the drivers of increased and diminished financial anxiety among young adults in Poland and Czechia.
Surgical robots: history, applications and future prospects
Authors: Bogue Rob 
Published: November 2024

Purpose
The purpose of this study is to provide details of the development and applications of surgical robots.

Design/methodology/approach
Following a short introduction, this first provides a historical background to surgical robot developments. The following sections discuss applications, specialised systems and their uses, remote telesurgery and a short consideration of future prospects. Finally, brief conclusions are drawn.

Findings
Catalysed by the needs of NASA and the US Department of Defence, robotic surgery was first conducted in the 1980s with modified industrial robots and the first dedicated surgical robots emerged commercially in the 1990s. These allow surgeons to conduct minimally invasive procedures which lead to significant patient benefits. Advances in communications have enabled long-range telesurgery based on commercial networks. In 2024, a telesurgical technique was conducted on simulated tissue on the International Space Station. Advances in artificial intelligence (AI), augmented reality and virtual reality will enhance the capabilities of future systems as well as contributing to improved surgeon training. In the longer term, AI-assisted surgery may be conducted with limited human intervention and full automation of certain procedures may ultimately arise.

Originality/value
This provides a detailed account of surgical robot developments and applications from their inception in the 1980s to the present day.