bluuebunny/crossref_metadata_2025_split huggingface dataset:
Individual tree traits shape insect and disease damage on oak in a climate‐matching tree diversity experiment
Authors: Field Elsa , Schönrogge Karsten , Barsoum Nadia , Hector Andrew , Gibbs Melanie 
Published: July 2019

AbstractDiversifying planted forests by increasing genetic and species diversity is often promoted as a method to improve forest resilience to climate change and reduce pest and pathogen damage. In this study, we used a young tree diversity experiment replicated at two sites in the UK to study the impacts of tree diversity and tree provenance (geographic origin) on the oak (Quercus robur) insect herbivore community and a specialist biotrophic pathogen, oak powdery mildew. Local UK, French, and Italian provenances were planted in monocultures, provenance mixtures, and species mixes, allowing us to test whether: (a) local and nonlocal provenances differ in their insect herbivore and pathogen communities, and (b) admixing trees leads to associational effects on insect herbivore and pathogen damage. Tree diversity had variable impacts on foliar organisms across sites and years, suggesting that diversity effects can be highly dependent on environmental context. Provenance identity impacted upon both herbivores and powdery mildew, but we did not find consistent support for the local adaptation hypothesis for any group of organisms studied. Independent of provenance, we found tree vigor traits (shoot length, tree height) and tree apparency (the height of focal trees relative to their surroundings) were consistent positive predictors of powdery mildew and insect herbivory. Synthesis. Our results have implications for understanding the complex interplay between tree identity and diversity in determining pest damage, and show that tree traits, partially influenced by tree genotype, can be important drivers of tree pest and pathogen loads.
Dynamic prediction of left ventricular assist device pump thrombosis based on lactate dehydrogenase trends
Authors: Hurst Thomas E. , Xanthopoulos Andrew , Ehrlinger John , Rajeswaran Jeevanantham , Pande Amol , Thuita Lucy , Smedira Nicholas G. , Moazami Nader , Blackstone Eugene H. , Starling Randall C. 
Published: July 2019

AbstractAimsThe risk of HeartMate II (HMII) left ventricular assist device (LVAD) thrombosis has been reported, and serum lactate dehydrogenase (LDH), a biomarker of haemolysis, increases secondary to LVAD thrombosis. This study evaluated longitudinal measurements of LDH post‐LVAD implantation, hypothesizing that LDH trends could timely predict future LVAD thrombosis.Methods and resultsFrom October 2004 to October 2014, 350 HMIIs were implanted in 323 patients at Cleveland Clinic. Of these, patients on 339 HMIIs had at least one post‐implant LDH value (7996 total measurements). A two‐step joint model combining longitudinal biomarker data and pump thrombosis events was generated to assess the effect of changing LDH on thrombosis risk. Device‐specific LDH trends were first smoothed using multivariate boosted trees, and then used as a time‐varying covariate function in a multiphase hazard model to analyse time to thrombosis. Pre‐implant variables associated with time‐varying LDH values post‐implant using boostmtree were also investigated. Standardized variable importance for each variable was estimated as the difference between model‐based prediction error of LDH when the variable was randomly permuted and prediction error without permuting the values. The larger this difference, the more important a variable is for predicting the trajectory of post‐implant LDH. Thirty‐five HMIIs (10%) had either confirmed (18) or suspected (17) thrombosis, with 15 (43%) occurring within 3 months of implant. LDH was associated with thrombosis occurring both early and late after implant (P < 0.0001 for both hazard phases). The model demonstrated increased probability of HMII thrombosis as LDH trended upward, with steep changes in LDH trajectory paralleling trajectories in probability of pump thrombosis. The most important baseline variables predictive of the longitudinal pattern of LDH were higher bilirubin, higher pre‐implant LDH, and older age. The effect of some pre‐implant variables such as sodium on the post‐implant LDH longitudinal pattern differed across time.ConclusionsLongitudinal trends in surveillance LDH for patients on HMII support are useful for dynamic prediction of pump thrombosis, both early after implant and late. Incorporating upward and downward trends in LDH that dynamically update a model of LVAD thrombosis risk provides a useful tool for clinical management and decisions.
Electrochemical Signal‐triggered Release of Biomolecules Functionalized with His‐tag Units
Authors: Bellare Madhura , Kadambar Vasantha Krishna , Bollella Paolo , Gamella Maria , Katz Evgeny , Melman Artem 
Published: July 2019

AbstractHis‐tagged molecular species, a ferrocene derivative and Protein A, were immobilized on electrode surfaces (Au and graphite) through formation of a chelated complex in the presence of Cu2+ cations used as bridging units. The complex was cleaved and the attached molecules were released from the electrode surface by applying reductive potential to the electrodes resulting in Cu2+ reduction, thus decomposing the chelate complex. The molecule release process was followed by cyclic voltammetry in case of the ferrocene derivative. His‐tagged Protein A was additionally labeled with a fluorescent tag and its release was followed by fluorescence measurements in the solution and by impedance spectroscopy at the electrode. The studied release of the His‐tagged redox species and biomolecules was considered as a new generic approach to the signal‐controlled molecule release applicable in various biotechnological and biomedical applications.
Migration characteristics and deep profile control mechanism of polymer microspheres in porous media
Authors: Li Jianbing , Niu Liwei , Lu Xiangguo 
Published: July 2019

AbstractThe performance and migration characteristics of polymer microspheres in porous media and the mechanism of deep profile control for oil displacement were studied by theoretical analysis, instrument detection, and physical simulation to further improve oil recovery. Polymer microspheres were expansive in water and nonexpansive in oil. The polymer microspheres with short expansion times possessed excellent mechanical shear resistance. According to the matching factor (Ra), resistance factor, and residual resistance factor, the migration of polymer microspheres in porous media was divided into four periods: transportation (Ra > 2.41), bridging plugging (1 < Ra < 2.41), elastic plugging (Ra < 1), and fatigue (microsphere breakage). The resistance factor and residual resistance factor of the polymer microspheres during the bridging and elastic plugging periods were larger than those during other periods. The deep oil displacement profile can be controlled by adjusting the occurrence positions (distance from the injection end) of these two periods. Increasing the injection rate during the transportation and bridging plugging periods and decreasing the injection rate during the elastic plugging period made elastic plugging occur farther from the injection end while maintaining the resistance factor and residual resistance factor at high levels. Aimed at the remaining oil accumulation area, the optimal profile control depth of oil displacement (distance from the injection end) of polymer microspheres in porous media can be regulated by changing the injection rate to enhance oil recovery. The simultaneous migration and expansion of polymer microspheres is the main mechanism of microsphere fluid diversion in porous media.
Predictive ability and economic gains from volatility forecast combinations
Authors: Fameliti Stavroula P. , Skintzi Vasiliki D. 
Published: July 2019

AbstractThe availability of numerous modeling approaches for volatility forecasting leads to model uncertainty for both researchers and practitioners. A large number of studies provide evidence in favor of combination methods for forecasting a variety of financial variables, but most of them are implemented on returns forecasting and evaluate their performance based solely on statistical evaluation criteria. In this paper, we combine various volatility forecasts based on different combination schemes and evaluate their performance in forecasting the volatility of the S&P 500 index. We use an exhaustive variety of combination methods to forecast volatility, ranging from simple techniques to time‐varying techniques based on the past performance of the single models and regression techniques. We then evaluate the forecasting performance of single and combination volatility forecasts based on both statistical and economic loss functions. The empirical analysis in this paper yields an important conclusion. Although combination forecasts based on more complex methods perform better than the simple combinations and single models, there is no dominant combination technique that outperforms the rest in both statistical and economic terms.
Enriched bovine IgG fraction prevents infections with Enterohaemorrhagic Escherichia coli O157:H7, Salmonella enterica serovar Enteritidis, and Mycobacterium avium
Authors: Funatogawa Keiji , Tada Tatsuya , Kuwahara‐Arai Kyoko , Kirikae Teruo , Takahashi Masao 
Published: July 2019

AbstractA bovine IgG‐enriched whey fraction contains antibodies against various bacterial antigens. We investigated the protective effects of a bovine whey fraction preparation against infections with Enterohaemorrhagic Escherichia coli O157:H7, Salmonella enterica serovar Enteritidis, and Mycobacterium avium in mouse models. After infection with these pathogens, the IgG‐enriched fraction or skim milk was given ad libitum at a 5% solution instead of water. The mice given the IgG‐enriched fraction were significantly resistant to orally challenged EHEC O157:H7 (LD50: 4.0 × 105 CFU/mouse) infections compared with the mice given skim milk (LD50: <1.5 × 102 CFU/mouse). The mice given the IgG‐enriched fraction were also significantly resistant to orally challenged S. Enteritidis (LD50: 5.0 × 106 CFU/mouse) infections compared with the mice given skim milk (LD50: <2.5 × 101 CFU/mouse). When the mice were nasally infected with M. avium, the numbers of the bacteria in lungs of mice given the IgG‐enriched fraction were significantly lower than those given skim milk 2 and 3 weeks after infection. These results strongly indicate that oral administration of the bovine IgG‐enriched whey fraction protects mice against food‐borne infection and also that it partially protects mice against respiratory tract infection.
Medical diagnoses among infants at entry in out‐of‐home care: A Swedish population‐register study
Authors: Högberg Ulf , Sennerstam Roland , Wester Knut , Högberg Göran , Andersson Jacob , Thiblin Ingemar 
Published: July 2019

AbstractBackground and aimsIdentification of child abuse involves a medical investigation and assessment of problems related to social environment and upbringing and might necessitate out‐of‐home care. The objective of this study was to analyse infants placed in out‐of‐home care in Sweden by incidence, medical diagnoses, and perinatal factors.MethodsThis was a population‐based register study of infants born in Sweden 1997 to 2014. Data were retrieved from registers at the Swedish National Board of Health and Welfare and Statistics Sweden. Outcome measures were out‐of‐home care categories: (a) “Problems Related to Social Environment/Upbringing”, (b) “Abuse diagnoses without SDH (subdural haemorrhage), RH (retinal haemorrhage), rib fracture, or long bone fracture”, and (c) “SDH, RH, rib fracture, or long bone fracture.” As a reference population, we randomly selected infants without medical diagnoses born the same year.ResultsOverall incidence of out‐of‐home care was 402 per 100 000. For subcategories (a), (b), and (c), the incidences were 14.8 (n = 273), 3.77 (n = 70), and 9.83 (n = 182) per 100 000, respectively. During the study period, the first remained unchanged; the latter two have been increasing. Compared with other reasons for out‐of‐home care, children in category (c), “SDH, RH, rib fracture, or long bone fracture”, had increased odds of being boys (adjusted odds ratio [aOR] 1.60; 95% confidence interval [CI], 1.08‐2.38) and decreased odds of having a mother being single (aOR 0.49; 95% CI, 0.32‐0.75) and a smoker (aOR 0.60; 95% CI, 0.37‐0.96). Compared with the reference population, children in this category were more often twin born (7.7% versus 2.8%), preterm (18.5% versus 5.5%), and small‐for‐gestational age (5.2% versus 2.1%).ConclusionSDH, RH, rib fracture, or long bone fracture constitute a minor part of medical diagnoses for infants entered in out‐of‐home care, but have been increasing, both in numbers and proportion. Overdiagnosis of abuse might be a possible reason but cannot be ascertained by this study design.
Oleanolic acid exerts inhibitory effects on the late stage of osteoclastogenesis and prevents bone loss in osteoprotegerin knockout mice
Authors: Zhao Dongfeng , Shu Bing , Wang Chenglong , Zhao Yongjian , Cheng Weidong , Sha Nannan , Li Chenguang , Wang Qiang , Lu Sheng , Wang Yongjun 
Published: July 2019

AbstractPostmenopausal women undergo rapid bone loss, which caused by the accelerated osteoclastic bone resorption. Receptor activator of nuclear factor kappa‐B ligand (RANKL) plays critical and essential roles on varied stages of osteoclastogenesis. Oleanolic acid (OA), a naturally derived small compound, has been found suppress osteoclastogenesis in early stage of bone marrow macrophages (BMMs). However, whether OA also regulates the late stage of osteoclastogenesis remains unclear. Here, the regulatory effect of OA on the late stage of osteoclastogenesis was investigated in vitro using RANKL‐pretreated BMMs and in vivo using osteoprotegerin (OPG) knockout mice. Our in vitro studies demonstrate that OA inhibits the late stage of osteoclastogenesis from RANKL‐pretreated BMMs. For in vivo animal investigation, OA attenuates the bone loss phenotypes in OPG‐knockout mice by decreasing the densities of osteoclast, which are in consistent with the finding with in vitro osteoclastogenesis. Mechanistic investigations found that OA largely inhibit the activity of c‐Fos and Nuclear factor of activated T‐cells c1 (NFATc1) with RANKL‐pretreated BMMs and OPG‐knockout mice. Furthermore, OA suppresses the activities of osteoclast genes, such as Tartrate resistant acid phosphatase (TRAP), CathepsinK (Ctsk), and Matrix metalloproteinase 9 (MMP9). Taken together these findings, they have not only defined an inhibitory effect of OA in the late stage of osteoclastogenesis but have also gained new molecular mechanisms underlying the process of osteoclast formation.
Declination of long noncoding RNA paternally expressed gene 10 inhibits A375 cells proliferation, migration, and invasion via mediating microRNA‐33a
Authors: Fu Yan , Bi Yiming , Wang Fang , Chen Xingxiu , Liu Huiling 
Published: July 2019

AbstractThe importance of long noncoding RNAs (lncRNAs) has been certified in malignant melanoma. Nonetheless, the functions of lncRNA paternally expressed gene 10 (PEG10) in malignant melanoma remain uninvestigated. This research discloses the influence of PEG10 in the biological actions of malignant melanoma cells. The sh‐PEG10 plasmid was transfected into A375 cells; meanwhile, the effects of declined PEG10 on cell viability, apoptosis, migration, invasion, and the correlative protein levels were probed. The miR‐33a expression in sh‐PEG10‐transfected cells was examined, and the above biological processes were studied again in miR‐33a inhibitor‐transfected A375 cells. Phosphoinositide 3‐kinase/protein kinase B (PI3K/AKT) and mechanistic target of rapamycin (mTOR) pathways were delved via Western blot. We found that the enhancement of PEG10 was discovered in melanoma tissues compared to related nonmelanoma tissues. Declination of PEG10 frustrated cell viability, repressed cyclinD1 and CDK4 expression, and triggered apoptosis, as well as suppressed migration and invasion in A375 cells. A negative correction between PEG10 and miR‐33a was confirmed, and repressed miR‐33a inverted the functions of PEG10 repression in A375 cells. In addition, PEG10 repression discouraged the activation of PI3K/AKT and mTOR pathways via elevation of miR‐33a. These results indicated that declination of PEG10 restrained A375 cell growth, migration, and invasion via adjusting miR‐33a and PI3K/AKT and mTOR pathways.
Mechanism of synthesis of anatase TiO2 pigment from low concentration of titanyl sulfuric–chloric acid solution under hydrothermal hydrolysis
Authors: Tian Ming , Liu Yahui , Wang Weijing , Zhao Wei , Chen Desheng , Wang Lina , Zhao Hongxin , Meng Fancheng , Zhen Yulan , Hu Zongyuan , Qi Tao 
Published: July 2019

AbstractIn order to investigate the influence of Cl−/SO42− molar ratios and hydrolysis temperature on the hydrolysis process and TiO2 pigment, H2TiO3 was prepared with a low concentration of titanyl sulfuric–chloric acid solution by hydrothermal hydrolysis. Under the optimal hydrolysis conditions, 1.5–2.2 μm of H2TiO3 samples were achieved. After doping and calcination, anatase TiO2 pigments demonstrated excellent performance: the achromic ability of tinctorial strength (TCS) and blue phase index (SCX) were 1,429 and 4.07, respectively. As hydrolysis was a significant step in the process, the structure was simplified to a periodic structure of Ti[OH](H2O)3Cl(SO4) to simulate the cluster structures. Based on experimental results and density functional theory (DFT) calculation, the hydrolysis mechanism was presumed to be a process of anionic (OH−, Cl− and SO42−) competition reaction to explain the formation of anatase‐type H2TiO3, and the crystal growth direction of H2TiO3 was also confirmed to be a (OA) and b (OB).
Mutation of CarO participates in drug resistance in imipenem‐resistant Acinetobacter baumannii
Authors: Zhu Li‐Jing , Chen Xiao‐Ying , Hou Pan‐Fei 
Published: July 2019

AbstractObjectiveAcinetobacter baumannii has become an important problem because of the high drug resistance rate. The aim of this study was to assess the antimicrobial resistance profile and explore the role of membrane porin in imipenem resistance of A baumannii.MethodsA total of 63 isolates of imipenem‐resistant A baumannii (IRAB) and 21 of imipenem‐sensitive A baumannii (ISAB) were collected. Susceptibility testing to 16 kinds of antimicrobial agents was conducted by K‐B method. PCR technique was used to detect carO and oprD genes, and sequencing was performed to compare the sequence between IRAB and ISAB. Three‐dimensional structure model of CarO protein was established.ResultsWhile ISAB isolates presented sensitive to most classes of antibiotics, isolates of IRAB displayed much higher resistance rate except tigecycline (3.2%), cefoperazone/sulbactam (28.6%), and minocycline (30.2%). All 84 isolates were observed carrying both carO and oprD genes. Further sequencing revealed important mutations of carO gene existed in IRAB in comparison with ISAB. Meanwhile, significant differences in three‐dimensional structure of carO protein molecule were also found between IRAB and ISAB.ConclusionsThe drug resistance profile of IRAB is increasingly severe in clinical settings. Mutation of CarO was identified as one of the molecular mechanisms involved in imipenem resistance in A baumannii.
Cav‐1 participates in the development of diabetic neuropathy pain through the TLR4 signaling pathway
Authors: Jia Gai‐Li , Huang Qi , Cao Yan‐Nan , Xie Ci‐Shan , Shen Yu‐Jing , Chen Jia‐Li , Lu Jia‐Hui , Zhang Mao‐Biao , Li Jun , Tao Yuan‐Xiang , Cao Hong 
Published: July 2019

AbstractThis study aims to determine whether caveolin‐1 (Cav‐1) participates in the process of diabetic neuropathic pain by directly regulating the expression of toll‐like receptor 4 (TLR4) and the subsequent phosphorylation of N‐methyl‐D‐aspartate receptor 2B subunit (NR2B) in the spinal cord. Male Sprague‐Dawley rats (120–150 g) were continuously fed with high‐fat and high‐sugar diet for 8 weeks, and received a single low‐dose of intraperitoneal streptozocin injection in preparation for the type‐II diabetes model. Then, these rats were divided into five groups according to the level of blood glucose, and the mechanical withdrawal threshold and thermal withdrawal latency values. The pain thresholds were measured at 3, 7, and 14 days after animal grouping. Then, eight rats were randomly chosen from each group and killed. Lumbar segments 4–6 of the spinal cord were removed for western blot analysis and immunofluorescence assay. Cav‐1 was persistently upregulated in the spinal cord after diabetic neuropathic pain in rats. The downregulation of Cav‐1 through the subcutaneous injection of Cav‐1 inhibitor daidzein ameliorated the pain hypersensitivity and TLR4 expression in the spinal cord in diabetic neuropathic pain (DNP) rats. Furthermore, it was found that Cav‐1 directly bound with TLR4, and the subsequent phosphorylation of NR2B in the spinal cord contributed to the modulation of DNP. These findings suggest that Cav‐1 plays a vital role in DNP processing at least in part by directly regulating the expression of TLR4, and through the subsequent phosphorylation of NR2B in the spinal cord.
Population Pharmacokinetics of Milrinone in Infants, Children, and Adolescents
Authors: Hornik Christoph P. , Yogev Ram , Mourani Peter M. , Watt Kevin M. , Sullivan Janice E. , Atz Andrew M. , Speicher David , Al‐Uzri Amira , Adu‐Darko Michelle , Payne Elizabeth H. , Gelber Casey E. , Lin Susan , Harper Barrie , Melloni Chiara , Cohen‐Wolkowiez Michael , Gonzalez Daniel ,   Best Pharmaceuticals for Children Act–Pediatric Trials Network Steering Committee
Published: July 2019

AbstractMilrinone is a type 3 phosphodiesterase inhibitor used to improve cardiac output in critically ill infants and children. Milrinone is primarily excreted unchanged in the urine, raising concerns for toxic accumulation in the setting of renal dysfunction of critical illness. We developed a population pharmacokinetic model of milrinone using nonlinear mixed‐effects modeling in NONMEM to perform dose‐exposure simulations in children with variable renal function. We included children aged <21 years who received intravenous milrinone per clinical care. Plasma milrinone concentrations were measured using a validated liquid chromatography–tandem mass spectrometry assay (range 1‐5000 ng/mL). We performed dose‐exposure simulations targeting steady‐state therapeutic concentrations of 100‐300 ng/mL previously established in adults and children with cardiac dysfunction. We simulated concentrations over 48 hours in typical subjects with decreasing creatinine clearance (CrCl), estimated using the updated bedside Schwartz equation. Seventy‐four patients contributed 111 plasma samples (concentration range, 4‐634 ng/mL). The median (range) postmenstrual age (PMA) was 3.7 years (0‐18), and median weight (WT) was 13.1 kg (2.6‐157.7). The median serum creatinine and CrCl were 0.5 mg/dL (0.1‐3.1) and 117.2 mL/min/1.73 m2 (13.1‐261.3), respectively. A 1‐compartment model characterized the pharmacokinetic data well. The final model parameterization was: Clearance (L/h) = 15.9*(WT [kg] / 70)0.75* (PMA1.12 / (67.71.12+PMA1.12)*(CrCl / 117)0.522; and Volume of Distribution (L) = 32.2*(WT [kg] / 70). A loading dose of 50 µg/kg followed by a continuous infusion of 0.5 µg/kg/min resulted in therapeutic concentrations, except when CrCl was severely impaired at ≤30 mL/min/1.73 m2. In this setting, a 25 µg/kg loading dose and 0.25 µg/kg/min continuous infusion resulted in therapeutic exposures.