bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deregulated lncRNA expression profile in the mouse lung adenocarcinomas with KRAS‐G12D mutation and P53 knockout
Authors: Zhang Meiqin , Jiang Nan , Cui Renjie , Du Sichen , Ou Huayuan , Chen Tinglan , Ge Runsheng , Ma Duan , Zhang Jin 
Published: August 2019

AbstractRecent studies have demonstrated that aberrant long non‐coding RNAs (lncRNAs) expression are suggested to be closely associated with multiple human diseases, lung cancer included. However, the roles of lncRNAs in lung cancer are not well understood. In this study, we used microarrays to investigate the aberrantly expressed lncRNAs in the mouse lung adenocarcinoma with P53 knockout and the KrasG12D mutation. Results revealed that 6424 lncRNAs were differentially expressed (≥ 2‐fold change, P < .05). Two hundred and ten lncRNAs showed more than 8‐fold change and conserved across human and were further analysed in the primary mouse lung adenocarcinoma KP cells, which were isolated from the p53 knockout and the KrasG12D mutation mice. Among all the 210 lncRNAs, 11 lncRNAs' expression was regulated by P53, 33 lncRNAs by KRAS and 13 lncRNAs by hypoxia in the primary KP cells, respectively. NONMMUT015812, which was remarkably up‐regulated in the mouse lung adenocarcinoma and negatively regulated by the P53 re‐expression, was detected to analyse its cellular function. Results showed that knockdown of NONMMUT015812 by shRNAs decreased proliferation and migration abilities of KP cells. Among those aberrantly expressed lncRNAs in the mouse lung adenocarcinoma, NONMMUT015812 was a potential oncogene.
Distribution patterns of saprolegniosis cutaneous lesions in wild and farmed brown trout (Salmo trutta L.) obtained using a geographic information system (GIS)
Authors: Aller‐Gancedo José‐Miguel , Fregeneda‐Grandes Juan‐Miguel 
Published: August 2019

AbstractA retrospective study was conducted using 250 clinical records of brown trout (Salmo trutta L.) with saprolegniosis by Saprolegnia parasitica, which had been collected from 8 rivers and 1 fish farm in the province of León (Spain). A geographic information system (GIS) was used to obtain skin lesion distribution patterns in males and females. Lesions in wild brown trout affected 15.31 ± 13.33% of the body surface, with a mean of 12.76 ± 6.56 lesions per fish. In addition, 51.23% of wild trout presented lesions with necrosis of the skin or fins. The pattern obtained when not distinguishing between sexes indicated that saprolegniosis lesions are mainly located above the lateral line and most frequently affect the dorsal cephalic region, the adipose fin, the peduncle and the caudal fin. However, differences were observed between males and females. Farmed trout presented a lower percentage of affected body surface (2.06 ± 4.36) and a lower number of lesions with and without necrosis because they received preventive treatment for saprolegniosis.
Ultrasound assisted hydration improves the quality of the malt barley
Authors: Borsato Viviani M. , Jorge Luiz M. M. , Mathias Alvaro L. , Jorge Regina M. M. 
Published: August 2019

AbstractThe purpose of this work was to describe the effect of thermal hydration sonication, and non‐sonication, of ANAG 01 barley grains at 35 and 40°C, to evaluate the malt quality parameters and the water incorporation capacity. The sonication caused an increase in the size of starch granules over time and improved the water incorporation rate. No fragmentation was identified by the application or absence of the ultrasound. The malt quality parameters were compared, and the best characteristics were obtained by the thermal treatments, sonication and non‐sonication, at 35°C, producing malt with most of the quality specifications required by the industry and suitable to be used in the formulation of malting blends. The process was accelerated with the increase in temperature and the application of ultrasound, comparing the moisture gain in 480 and 720 min of hydration. The Peleg model was suitable to describe the hydration kinetics.Practical ApplicationsUsing temperatures of 35 and 40°C higher than tipical values in the industrial hydration operation requires less time. The association with the ultrasound is even faster, but can influence the quality of the malt. Therefore, sonication at 35°C temperature was a good alternative to produce malt from barley (ANAG 01).
Life cycle assessment of 3D printing geo‐polymer concrete: An ex‐ante study
Authors: Yao Yue , Hu Mingming , Di Maio Francesco , Cucurachi Stefano 
Published: August 2019

AbstractThree‐dimensional (3D) printing and geo‐polymers are two environmentally oriented innovations in concrete manufacturing. The 3D printing of concrete components aims to reduce raw material consumption and waste generation. Geo‐polymer is being developed to replace ordinary Portland cement and reduce the carbon footprint of the binder in the concrete. The environmental performance of the combined use of the two innovations is evaluated through an ex‐ante life cycle assessment (LCA). First, an attributional LCA was implemented, using data collected from the manufacturer to identify the hotspots for environmental improvements. Then, scaled‐up scenarios were built in collaboration with the company stakeholder. These scenarios were compared with the existing production system to understand the potential advantages/disadvantages of the innovative system and to identify the potential directions for improvement.The results indicate that 3D printing can potentially lead to waste reduction. However, depending on its recipe, geo‐polymer likely has higher environmental impacts than ordinary concrete. The ex‐ante LCA suggests that after step‐by‐step improvements in the production and transportation of raw materials, 3D printing geo‐polymer concrete is able to reduce the carbon footprint of concrete components, while it does still perform worse on impact categories, such as depletion of abiotic resources and stratospheric ozone depletion. We found that the most effective way to lower the environmental impacts of 3D concrete is to reduce silicate in the recipe of the geo‐polymer. This approach is, however, challenging to realize by the company due to the locked‐in effect of the previous innovation investment. The case study shows that to support technological innovation ex‐ante LCA has to be implemented as early as possible in innovation to allow for maintaining technical flexibility and improving on the identified hotspots.
Regional material flow accounts for China: Examining China's natural resource use at the provincial and national level
Authors: Wang Heming , Schandl Heinz , Wang Guoqiang , Ma Lin , Wang Yao 
Published: August 2019

AbstractOver the last three decades, China has experienced the most dynamic economic development lifting living standards and resulting in fast‐growing use of natural resources. In the past, the focus has been on national MFA accounts which do not do justice to the second largest economy, home to 19% of the world population and having 30% of global material use. In this research, we calculate material extraction for China at the regional level during 1995–2015 using the most recent available statistical data and applying the most up‐to‐date international calculation methods. In particular, we combine a bottom‐up and top‐down approach for constructing the dataset of China's economically used Domestic Extraction (DEU) in an integrated way. This approach also improves the Chinese national material flow accounts and allows us to present a reliable database of DE of materials for China to date. Our new dataset provides the basis for calculating material footprints and environmental impacts of China's regions. The dataset enables us to evaluate regional resource efficiency trends in China. We find that during the past two decades, China's material use has grown strongly from 11.7 billion tonnes in 1995 to 35.4 billion tonnes in 2015. Material use has accelerated between 2000 and 2010 but slowed down between 2010 and 2015 reflecting the economic contraction caused by the Global Financial Crisis which reduced the global demand for China's manufacturing and a reorientation of China's economic policy settings toward quality of growth. Unsurprisingly, different regions play different roles in the supply chain of materials, achieving different economic performances resulting in very diverse material efficiency outcomes. This information is important to allow for a targeted policy approach to increase resource efficiency, reduce environmental impacts of resource use, and grow wellbeing in China with large positive implications for global sustainability. This study provides the basis for the development of relevant resource management policies for different regions in the future.
Evidence of an Association Between Vitamin D Deficiency and Preterm Birth and Preeclampsia: A Critical Review
Authors: Woo Jennifer , Giurgescu Carmen , Wagner Carol L. 
Published: August 2019

AbstractVitamin D deficiency has been associated with adverse pregnancy and birth outcomes such as increased risk for preterm birth and preeclampsia. This state of the science review analyzed recently published meta‐analyses and relevant studies that have evaluated the association between vitamin D deficiency and preeclampsia or preterm birth. The results suggest that a positive association between vitamin D deficiency and preterm birth exists. However, the findings of the relationship between vitamin D deficiency and preeclampsia were inconclusive, possibly because of the need for supplementation to occur prior to placentation. This may be because of a lack of studies with ethnic minority populations, who are more likely to experience vitamin D deficiency, and inadequate supplementation doses used for treatment of vitamin D deficiency. Health care providers should screen pregnant women at risk for vitamin D deficiency and supplement women accordingly based on their vitamin D status. Lastly, well‐designed and standardized clinical trials need to include large cohorts of minority pregnant women to establish the impact of vitamin D supplementation on improving preterm birth and preeclampsia risk in pregnancy.
Clinical and neurophysiological characteristics of patients with Guillain‐Barré syndrome at Hospital Universitario San Ignacio, Bogotá, Colombia between 2009 and 2017
Authors: Millán Sonia , Díaz Cindy , David Pardo David G. , Castro Guzmán Gabriel 
Published: August 2019

AbstractGuillain‐Barré syndrome (GBS) is the most common acute peripheral polyneuropathy in the world. The estimated incidence in Colombia is 1.2‐1.7 cases per 100 000 inhabitants, although during 2016 an increase in the incidence of the disease was documented, apparently associated with an epidemiological peak of the Zika virus. We conducted to describe the clinical and neurophysiological characteristics of adult patients with GBS treated at Hospital Universitario San Ignacio, Bogota, Colombia, between 2009 and 2017. An observational, descriptive, cross‐sectional study was designed.
Psychopathology among individuals seeking minimally invasive cosmetic procedures
Authors: Özkur Ezgi , Kıvanç Altunay İlknur , Aydın Çiğdem 
Published: August 2019

AbstractObjectivesIn recent years, so many people want to alter their physical appearance with the purpose of raising their social and psychological well‐being and the demand for minimally invasive cosmetic procedures (MICPs) has continued to rise. Our study aims to investigate the psychological profile of people seeking cosmetic procedures.Study DesignThe present observational cross‐sectional study was conducted with a sample of 54 participants seeking cosmetic procedures (botulinum toxin injections, soft tissue filler injection, mesotherapy, platelet‐rich plasma, and dermaroller treatments). Those patients were compared to the control group, who did not have any kind of cosmetic procedure (including cosmetic surgery) before and who did not want to have any of these procedures.MethodsThe research volunteers were invited to complete the demographic questionnaire (e.g., age, gender and history of procedures) as well as psychological scales. Psychological scales includes the validated and reliable The Brief Symptom Inventory (BSI), Automatic Thoughts Scale (ATS), and Social Adaptation Self‐Evaluation Scale (SASS).ResultsUsers of MICP were mostly female (n = 46, 85%) and had some high school education or higher and showed higher scores on General Severity Index (P = .013), anxiety (P = .018), depression (P = .004), interpersonal sensitivity (P = .008) of BSI and also higher on ATS (P = .022) and lower on SASS (P = .001) scores that mean less social adaptation. There was a statistically positive correlation between age and GSI, anxiety, depression, interpersonal sensitivity, somatization scores of BSI, and negative correlations between SASS scores and age and number of past procedures.ConclusionOur study findings highlight the importance of understanding individuals’ psychological symptoms who are seeking cosmetic procedures.
From organisational justice to turnover intention among community nurses: A mediating model
Authors: Huang Xiuxiu , Li Zhaoyang , Wan Qiaoqin 
Published: August 2019

AbstractAims and objectivesTo identify the situation of organisational justice, organisational support, work engagement and turnover intention among community nurses and clarify the relationship among them.BackgroundNurse shortage has become a worldwide issue, and the shortage of community nurses is more serious. Nurse turnover is one important factor for this situation. While previous studies about turnover intention mostly focused on hospital nurses, few involved community nurses.DesignA descriptive, cross‐sectional design was adopted.MethodsQuestionnaire investigation was implemented among 410 community nurses to collect data about organisational justice, organisational support, work engagement and turnover intention. Structural equation modelling was applied to test the hypothesised model. The STROBE statement for observational studies was followed.ResultsResults showed the average score of turnover intention was 2.50 ± 0.75. The final model indicated: (a) work engagement had a direct negative effect on turnover intention; (b) organisational support had both direct and indirect effects on turnover intention, and the indirect effect was mediated by work engagement; and (c) organisational justice had an indirect effect on turnover intention mediated by organisational support.ConclusionTurnover intention could be reduced directly or indirectly by improving work engagement, organisational support and organisational justice.Relevance to clinical practiceThis study provides evidence for community health centre managers and policymakers to improve the level of nurses' engagement and to retain more nurses in primary healthcare service.
Expression and clinical value of gastrin‐releasing peptide precursor in nephropathy and chronic kidney disease
Authors: Dai Zhang , Zhu Jianhui , Huang Huibin , Fang Lili , Lin Yongzhi , Huang Songjie , Xie Fang , Sheng Nan , Liang Xianming 
Published: August 2019

ABSTRACTAimTo explore whether serum pro‐gastric releasing peptide (proGRP) is elevated in nephropathy patients and evaluate the diagnostic value of proGRP in chronic kidney disease (CKD) patients.MethodsA total of 498 nephropathy patients and 170 healthy were selected in Zhongshan Hospital, Medical College of Xiamen University, from February 2016 to September 2017. The clinical data of the different groups including serum proGRP, CKD grading, and other serum and urine renal function biomarkers were analyzed by group comparison, correlation analysis and receiver operating characteristic curve.ResultsSerum proGRP levels were significantly higher in the acute kidney injury and CKD groups compared with the other groups of kidney disease patients (P < 0.01), and increased with CKD grading (P < 0.01). Serum proGRP was substantially correlated with serum creatinine (r = 0.637, P < 0.01) and cystain C (0.837, P < 0.01). Serum proGRP had moderate correlations with urine β2‐macroglobulin (β2‐m; r = 0.587, P < 0.01) and α1‐macroglobulin (α1‐m; r = 0.557, P < 0.01). There were fair associations of serum proGRP with albumin (r = 0.10, P = 0.067), 24 h proteinuria (24 h‐TPU; r = 0.092, P = 0.099), urinary albumin/urocreatinine (uAlb/Cr; r = 0.29, P < 0.01) and urinary N‐acetyl‐β‐D‐glucosidase (r = −0.142, P < 0.01). The sensitivity of proGRP was superior to that of simplified modification of diet in renal disease (MDRD) formula in diagnosing CKD I + II (81.25% vs 66.67%), CKD III (86.42% vs 74.36%) and CKD IV (71.19% vs 69.64%), while its specificity was inferior to that of simplified MDRD formula in diagnosing CKD I + II (37.65% vs 66.97%), CKD III (56.25% vs 86.67%) and CKD IV (75.31% vs 88.46%).ConclusionSerum proGRP is elevated in acute renal injury and CKD patients and increases with CKD grading. Serum proGRP is mainly affected by glomerular filtration rate and could be used for CKD staging, although the overall diagnostic sufficiency is inferior to simplified MDRD formula.
Thermal imaging as a noninvasive technique for analyzing circadian rhythms in plants
Authors: Dakhiya Yuri , Green Rachel M. 
Published: August 2019

Summary

Endogenous (˜24 circadian) rhythms control an enormously diverse range of processes in plants and are, increasingly, the target of studies aimed at understanding plant performance. Although in the previous few decades most plant circadian research has focused on Arabidopsis, there is a pressing need for low‐cost, high‐throughput tools for analyzing rhythms in a wider variety of species. The present contribution investigates using circadian temperature oscillations as a novel marker for assaying plant circadian rhythms.

A thermal imaging platform was set up to measure diel and circadian rhythms in different plant species, in wild‐type and circadian mutant plants, and in leaves and flowers. Results from the thermal imaging technique were compared with those from other established circadian assay techniques.

All of the dicot and monocot species examined showed robust circadian rhythms of leaf surface temperature; the effects of circadian mutations on thermocycles were similar to those reported using other techniques. In Petunia × atkinsiana plants circadian oscillations were observed in both leaves and flowers.

Thermal imaging is an extremely useful technique for analyzing circadian rhythms in plants. It is predicted that the ability to make very high temporal resolution measurements may facilitate the discovery of novel aspects of circadian control.
A decade of Tropical Legumes projects: Development and adoption of improved varieties, creation of market‐demand to benefit smallholder farmers and empowerment of national programmes in sub‐Saharan Africa and South Asia
Authors: Varshney Rajeev K. , Ojiewo Chris , Monyo Emmanuel 
Published: August 2019

AbstractThis article highlights 12 years (2007–2019) of research, achievements, lessons learned, challenges and gaps in discovery‐to‐delivery research in legumes emanating from three projects, collectively called Tropical Legumes (TL) with a total investment of about US$ 67 million funded by the Bill & Melinda Gates Foundation. These projects were implemented by three CGIAR centres (ICRISAT, CIAT and IITA) together with 15 national agricultural research system partners in sub‐Saharan Africa and South Asia. The TL projects together with some of their precursors and complementary projects from other agencies, facilitated the development of 266 improved legume varieties and the production of about 497,901 tons of certified seeds of the target legume crops in the focus countries. The certified seeds have been planted on about 5.0 million ha by more than 25 million smallholder farmers in the 15 countries and beyond, producing about 6.1 million tons of grain worth US$ 3.2 billion. Furthermore, the projects also trained 52 next generation scientists that included 10 women, by supporting 34 Masters degrees and 18 PhD degrees.
Fighting on two fronts: Elevated insect resistance in flooded maize
Authors: Block Anna K. , Hunter Charles T. , Sattler Scott E. , Rering Caitlin , McDonald Samantha , Basset Gilles J. , Christensen Shawn A. 
Published: August 2019

AbstractTo grow and thrive plants must be able to adapt to both adverse environmental conditions and attack by a variety of pests. Elucidating the sophisticated mechanisms plants have developed to achieve this has been the focus of many studies. What is less well understood is how plants respond when faced with multiple stressors simultaneously. In this study, we assess the response of Zea mays (maize) to the combinatorial stress of flooding and infestation with the insect pest Spodoptera frugiperda (fall armyworm). This combined stress leads to elevated production of the defence hormone salicylic acid, which does not occur in the individual stresses, and the resultant salicylic acid‐dependent increase in S. frugiperda resistance. Remodelling of phenylpropanoid pathways also occurs in response to this combinatorial stress leading to increased production of the anti‐insect C‐glycosyl flavones (maysins) and the herbivore‐induced volatile phenolics, benzyl acetate, and phenethyl acetate. Furthermore, changes in cellular redox status also occur, as indicated by reductions in peroxidase and polyphenol oxidase activity. These data suggest that metabolite changes important for flooding tolerance and anti‐insect defence may act both additively and synergistically to provide extra protection to the plant.
Non‐invasive discrimination of acute focal bacterial nephritis with pyelonephritis
Authors: Oka Hideharu , Nagamori Tsunehisa , Yamamoto Shiho , Manabe Hiromi , Taketazu Genya , Mukai Tokuo , Sakata Hiroshi , Oki Junichi 
Published: August 2019

AbstractBackgroundThe appropriate antimicrobial treatment period for febrile urinary tract infection (UTI) can be changed, depending on whether the patient has acute focal bacterial nephritis (AFBN). The aim of this study was to clarify the characteristics of AFBN compared with those of acute pyelonephritis (APN) and establish a strategy to detect AFBN.MethodsA total of 77 patients diagnosed with febrile UTI were enrolled. They were divided into APN (n = 64) and AFBN groups (n = 13). The clinical data and other laboratory biomarkers were retrospectively analyzed.ResultsThe time required for fever resolution after antimicrobial treatment was significantly longer in the AFBN group than in the APN group (2.77 days vs 1.11 days, respectively, P < 0.001). Also, the time to disappearance of pyuria after antimicrobial treatment was longer in the AFBN group than in the APN group (6.22 days vs 2.32 days, respectively, P = 0.001). Fever lasting >1.75 days after antimicrobial treatment had a sensitivity of 92% and specificity of 79% for the detection of AFBN, while pyuria disappearance after 4 days had a sensitivity of 88% and specificity of 85%. When patients fulfilled both cut‐offs, the sensitivity and specificity were 89% and 97%.ConclusionAcute focal bacterial nephritis was associated with fever of significantly longer duration after antimicrobial treatment, and it took a longer time for pyuria to disappear. Children with febrile UTI should be evaluated for AFBN if the fever persists ≥48 h after the initiation of antimicrobial treatment and if pyuria lasts for 4 days.