bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pathogenicity of Pratylenchus penetrans to dwarfing apple rootstocks
Authors: Bélair Guy , Dauphinais Nathalie , Fournier Yvon 
Published: June 2019

The objective of this research was to measure the effect of P. penetrans densities on the vegetative growth of three apple dwarfing rootstocks Bud 9, M.9 and M.26 under field microplot conditions. Under 500 nematodes kg-1 soil density, the shoot dry weights of Bud 9, M.9 and M.26 rootstocks were significantly reduced by 30%, 23% and 14% respectively. Under the 1 500 nematodes kg-1 soil density, the dry shoot weight of Bud 9 and M.9 were not significantly different from the 500 nematodes kg-1 soil density except M.26 which exhibited a 29% decrease. The root dry weights of rootstocks were not significantly reduced when exposed to these P. penetrans densities except M.26 which was reduced 26% relative to non-inoculated trees under the 1 500 nematodes kg-1 soil density. These results confirm the pathogenic effect of P. penetrans on dwarfing apple rootstocks. Although preliminary, these results suggest some tolerance of M.26 to P. penetrans under lower density when compared to M.9 and Bud 9. Under higher nematode densities, both Bud 9 and M.9 exhibited a tolerance of their root system in comparison to M.26. Our results also emphasize the need to further assess the susceptibility/tolerance of dwarfing apple rootstocks to various densities of P. penetrans.
La répartition du conopholis d’Amérique (Conopholis americana [L.] Wallroth) au Québec
Authors: Deshaye Jean , Landry Tommy , Goudreau Réal , Dubois Maïtée , Grimard Marie-Josée 
Published: June 2019

Au Québec, le conopholis d’Amérique (Conopholis americana [L.] Wallr.) est une plante parasite des racines du chêne rouge (Quercus rubra L.). Désignée espèce vulnérable au Québec, cette plante est beaucoup plus rare que son hôte et se restreint à quelques versants de collines exposés au sud. À la suite de la découverte d’une population totalisant près de 2 000 tiges dans la région de Rawdon, 2 hypothèses ont été explorées pour tenter d’expliquer la répartition de l’espèce, soit : 1) des inventaires insuffisants, 2) la qualité de l’habitat. Selon les résultats obtenus, aucune de ces hypothèses n’apparaît satisfaisante. Des facteurs aléatoires, comme la dispersion par l’ours noir ou le cerf de Virginie, pourraient être en cause.
M
ethyloparacoccus
Authors: Vekeman Bram 
Published: June 2019

Abstract

Me.thy.lo.pa.ra.coc'cus. N.L. neut. n.
methylum
methyl; N.L. pref.
methylo
‐ pertaining to the methyl radical; Gr. prep.
para
, beside, alongside of, near, like; N.L. masc. n.
coccus
(from Gr. n.
kokkos
), a grain or berry; N.L. masc. n.
Methyloparacoccus
referring to a methyl‐using organism resembling but clearly different from other methyl‐using cocci.

Proteobacteria / Gammaproteobacteria / Methylococcales / Methylococcaceae / Methyloparacoccus

Cells are aerobic, Gram‐negative, nonmotile coccoids, 0.8–1.5 µm in diameter. Cells occur singly with a diplococcoid tendency. Reproduce by normal cell division. Resting stages are not observed. Obligate utilizers of methane and methanol as sole carbon and energy sources. Methane is oxidized by a particulate methane monooxygenase (pMMO); the soluble methane monooxygenase (sMMO) and the alternative particulate monooxygenase (pXMO) are absent. Cells possess the typical intracytoplasmic membrane system for gammaproteobacterial methanotrophs forming bundles of membrane vesicles. No growth occurs on compounds containing carbon–carbon bonds. Atmospheric nitrogen is not fixed. Cells are neutrophilic, growing between pH 5.8 and 9, with an optimal pH of 6.3–6.8 and mesophilic, nonthermotolerant, growing between 20 and 37°C, optimal growth temperature 25–33°C. The dominant cellular fatty acids are

C
16:1

ω7
c
(52–54%) and

C
16:0

(24–25%). So far only isolated from pond water in Africa and Japan. Belongs to the
Gammaproteobacteria
as part of the order
Methylococcales
, family
Methylococcaceae
.

DNA G + C content (mol%)
: 65.6 (
T
m
).

Type species
:
Methyloparacoccus murrellii
Hoefman, van der Ha, Iguchi, Yurimoto, Sakai, Boon, Vandamme, Heylen and De Vos, 2014b, 2105
VP
.
L
ysinibacillus
Authors: Failor Kevin C. , Tian Long , Monteil Caroline L. , Vinatzer Boris A. 
Published: June 2019

Abstract

Ly.si.ni.ba.cil'lus. N.L. neut. n.
lysinum
lysine; L. masc. n.
bacillus
a small staff or rod; N.L. masc. n.
Lysinibacillus
lysine bacillus, referring to the presence of the Lys–Asp type of peptidoglycan in the cell wall.

Firmicutes / Bacilli / Bacillales / Bacillaceae / Lysinibacillus

The genus
Lysinibacillus
was proposed in 2007 to describe a phylogenetic and physiological distinct group of
Bacillaceae
that exhibit an
l
‐Lys‐
d
‐Asp (A4α) cell wall composition. Twenty‐one
Lysinibacillus
species were validly published between 2007 and 2018, and five former
Bacillus
species were moved to
Lysinibacillus
.
Lysinibacillus boronitolerans
serves as the type species.
Lysinibacillus
strains have been isolated from an impressive diversity of environments ranging from pristine and contaminated soils to plants, aquatic environments, indoor air, food, insects, animal waste, a cellular phone screen, and even an organic solvent. The genus is primarily Gram‐positive and aerobic with cells consisting in motile rods capable of forming terminally located bulging endospores. All species are catalase‐positive and typically have iso‐C
15:0
as the primary fatty acid and MK‐7 as the primary menaquinone system. Diphosphatidylglycerol and phosphatidylglycerol are the major polar lipids. The members of the genus
Lysinibacillus
cannot reduce nitrate, are unable to produce indole, and are incapable of utilizing 2‐ketogluconate, capric acid,
d
‐arabinose, gentiobiose, indole,
myo
‐inositol, stachyose, starch, β‐gentiobiose, and β‐methyl‐xyloside. While the
Lysinibacillus
genus is best known for a clonal lineage of mosquitocidal isolates because of their widespread use in mosquito control,
Lysinibacillus
strains also have potential in biotechnological applications and bioremediation because of their enzymatic capabilities and tolerance to a variety of pollutants.

DNA G + C content (mol%)
: 35–43.3.

Type species
:
Lysinibacillus boronitolerans
Ahmed, Yokota, Yamazoe and Fujiwara 2007, 1121
VP
.
Prognostic significance of baseline FLT3‐ITD mutant allele level in acute myeloid leukemia treated with intensive chemotherapy with/without sorafenib
Authors: Yalniz Fevzi , Abou Dalle Iman , Kantarjian Hagop , Borthakur Gautam , Kadia Tapan , Patel Keyur , Loghavi Sanam , Garcia‐Manero Guillermo , Sasaki Koji , Daver Naval , DiNardo Courtney , Pemmaraju Naveen , Short Nicholas J. , Yilmaz Musa , Bose Prithviraj , Naqvi Kiran , Pierce Sherry , Nogueras González Graciela M. , Konopleva Marina , Andreeff Michael , Cortes Jorge , Ravandi Farhad 
Published: June 2019

AbstractInternal tandem duplication (ITD) of the fms‐related tyrosine kinase‐3 gene (FLT3) confer a poor prognosis in adult AML. Studies have reported that a higher mutant allelic burden is associated with a worse prognosis. Adult patients with FLT3‐ITD mutated AML treated at our institution were identified. Patients were assigned into 2 groups; patients who received idarubicin and cytarabine (IA, group one) containing induction, and who received sorafenib in addition to IA containing regimens at induction (group two). The optimal FLT3‐ITD mutant allele cut‐off was defined as the cut‐off to divide the whole cohort with the highest statistical significance. A total of 183 patients including 104 (57%) in group one and 79 (43%) in group two were identified. The complete remission (CR)/CR with incomplete hematologic recovery (CRi) for group one and group two were 85% and 99%, respectively (P = .004). The median relapse free survival (RFS) for group one and two were 12 and 45 months, respectively (P = .02). The median overall survival (mOS) was 17 months in group one, and has not been reached in group two (P = .008). The optimal FLT3‐ITD mutant allele cut‐off for OS was 6.9% in group one, there was no optimal cut‐off in group two. On multivariate analysis, poor performance status (PS) (P = .003), sorafenib (P = .01), and presenting white blood cells (WBC) (P < .001) were independent predictors of OS. Higher FLT3‐ITD allele burden is associated with a worse outcome in patients treated with IA‐based chemotherapy. Addition of sorafenib to chemotherapy not only nullifies the negative prognostic impact of higher allele burden, but also improves outcome of FLT3‐ITD mutated AML patients regardless of the allele burden.
Network regulation and properties optimization of glycidyl azide polymer‐based materials as a candidate of solid propellant binder via alternating the functionality of propargyl‐terminated polyether
Authors: Qi Chun , Tang Gen , Guo Xiang , Liu Changhua , Pang Ai‐min , Gan Lin , Huang Jin 
Published: June 2019

ABSTRACTA kind of glycidyl azide polymer (GAP)‐based composite has been fabricated using propargyl‐terminated ethylene oxide‐tetrahydrofuran copolymer (PPET) with two (p‐) and three (t‐) alkyne functionalities via Huisgen reaction. Independent upon the PPET functionality, both crosslink densities and mechanical properties for two GAP/PPET systems showed a positive‐interrelation changes of initial increase and subsequent decrease with an increase of azide/alkyne molar ratios. At equivalent of azide/alkyne molar ratios, the composites containingt‐PPET with higher alkyne functionality exhibited better mechanical properties, while those with two alkyne functionality presented lower glass transition. Under the regulation of alkyne functionality as 3 and azide/alkyne molar ratio as 3:1, the tensile strength, Young's modulus and breaking elongation could simultaneously reach the maximum values of 1.38 MPa, 4.07 MPa, and 122.5%, which was ascribed to optimal participation of azide/alkyne reaction into network construction. Overall, this study provides an additional optimization route for network‐structured binders in solid propellant system. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci.2019,136, 48016.
When do peers influence adolescent males' risk taking? Examining decision making under conditions of risk and ambiguity
Authors: Lloyd Alex , Döring Anna K. 
Published: June 2019

AbstractRisk taking is highly prevalent among adolescent males, and a range of studies have shown that decisions become riskier if a peer is present. However, previous studies have typically provided participants with explicit probabilities of risk in each situation. This does not accurately reflect adolescents' real‐world risk taking, where decisions are made in ambiguous situations alongside their peers. Aiming for a more ecologically valid design, the present experiment manipulated situational ambiguity and examined its interplay with group decision making and developmental factors. Adolescent males (N = 202) aged 12–15 completed a “Wheel of Fortune” task and then self‐reported their score, presenting an opportunity to cheat as a measure of antisocial risk taking. As predicted, adolescents were more likely to take risks when probabilities were ambiguous rather than explicit. Further, higher levels of gambling choices were made by groups in ambiguous, but not risk situations. Age significantly predicted gambling in ambiguous conditions, whereas developmental dispositions (risk perception, reward sensitivity, and inhibitory control) did not play a role. Findings provide an insight into the social and situational conditions under which adolescent males engage in reckless behavior.
Lectin bio‐layer interferometry for assessing product quality of Fc‐ glycosylated immunoglobulin G
Authors: Wallner Jakob , Sissolak Bernhard , Sommeregger Wolfgang , Lingg Nico , Striedner Gerald , Vorauer‐Uhl Karola 
Published: June 2019

AbstractGlycosylation, as the most prominent posttranslational modification, is recognized as an important quality attribute of monoclonal antibodies affected by various bioprocess parameters and cellular physiology. A method of lectin‐based bio‐layer interferometry (LBLI) to relatively rank galactosylation and fucosylation levels was developed. For this purpose, Fc‐glycosylated immunoglobulin G (IgG) was recombinantly produced with varying bioprocess conditions in 15 L bioreactor and accumulated IgG was harvested. The reliability, the robustness and the applicability of LBLI to different samples has been proven. Data obtained from LC–MS analysis served as reference and were compared to the LBLI results. The introduced method is based on non‐fluidic bio‐layer interferometry (BLI), which becomes recently a standard tool for determining biomolecular interactions in a label‐free, real‐time and high‐throughput manner. For the intended purpose, biotinylated lectins were immobilized on disposable optical fiber streptavidin (SA) biosensor tips. Aleuria aurantia lectin (AAL) was used to detect the core fucose and Ricinus communis agglutinin 120 (RCA120) to determine galactosylation levels. In our case study it could be shown that fucosylation was not affected by variations in glucose feed concentration and cultivation temperature. However, the galactosylation could be correlated with the ratio of mean specific productivity (qP) and ammonium (qNH4+) but was unrelated to the ratio of mean qP and the specific glucose consumption (qgluc). This presented method strengthens the applicability of the BLI platform, which already enables measurement of several product related characteristics, such as product quantity as well as kinetic rates (kd,kon) and affinity constants (kD) analysis.
ATLANTIC MAMMALS: a data set of assemblages of medium‐ and large‐sized mammals of the Atlantic Forest of South America
Authors: Souza Yuri , Gonçalves Fernando , Lautenschlager Laís , Akkawi Paula , Mendes Calebe , Carvalho Mariana M. , Bovendorp Ricardo S. , Fernandes‐Ferreira Hugo , Rosa Clarissa , Graipel Maurício Eduardo , Peroni Nivaldo , Cherem Jorge José , Bogoni Juliano André , Brocardo Carlos Rodrigo , Miranda João , Zago da Silva Luciana , Melo Geruza , Cáceres Nilton , Sponchiado Jonas , Ribeiro Milton Cezar , Galetti Mauro 
Published: June 2019

AbstractBiodiversity inventories contain important information about species richness, community structure, and composition, and are the first step in developing any conservation and mitigation strategies. The Atlantic Forest of South America is home to around 334 species of small‐, medium‐, and large‐sized mammals, and is currently restricted to less than 12% of its original cover. Here, we present the ATLANTIC MAMMALS, an open data set on information on medium‐ and large‐sized mammal assemblages in the Atlantic Forest of Brazil, Paraguay, and Argentina. A total of 129 studies were compiled, including published and in press peer‐reviewed papers, book chapters, theses and unpublished data. We mapped 244 assemblages, eight orders, 63 genera, and 94 species (24 of which are classified as threatened by the IUCN Red List) distributed in 128 protected and 116 unprotected areas. Species richness of the mammalian assemblages varied from 1 to 39 species (mean 15). The most recorded species in the entire biome was Dasypus novemcinctus, followed by Cerdocyon thous and Procyon cancrivorous. These data can be useful in support of macroecological studies and conservation planning strategies. Please cite this data paper when the data are used in publications. We also request that researchers and teachers inform us of how they are using the data.
Hitting Reset on Sediment Toxicity: Sediment Homogenization Alters the Toxicity of Metal-Amended Sediments
Authors: Costello David M. , Harrison Anna M. , Hammerschmidt Chad R. , Mendonca Raissa M. , Burton G. Allen 
Published: June 2019

Abstract
Laboratory testing of sediments frequently involves manipulation by amendment with contaminants and homogenization, which changes the physicochemical structure of sediments. These changes can influence the bioavailability of divalent metals, and field and mesocosm experiments have shown that laboratory-derived thresholds are often overly conservative. We assessed the mechanisms that lead to divergence between laboratory- and field-derived thresholds; specifically, we assessed the importance of slow equilibration to solid-phase ligands and vertical stratification. To mimic natural physicochemical conditions, we uniquely aged sediment with a flow-through exposure system. These sediments were then homogenized and compared, toxicologically, with freshly metal-amended sediments in a 28-d chronic toxicity bioassay with the amphipod Hyalella azteca. We assessed concentration–response relationships for 3 metals (copper, nickel, and zinc) and 5 geochemically distinct sediments. We observed minimal differences in growth and survival of H. azteca between aged and freshly spiked sediments across all sediments and metals. These trends suggest that a loss of toxicity observed during long-term sediment aging is reversed after sediment homogenization. By comparison with mesocosm experiments, we demonstrate that homogenizing sediment immediately before toxicity assays may produce artificially high toxicity thresholds. We suggest that toxicity assays with sediments that maintain vertical redox gradients are needed to generate field-relevant sediment metal toxicity thresholds. Environ Toxicol Chem 2019;38:1995–2007. © 2019 SETAC.
Abstract
Aging of sediment alone does not alter metal toxicity to amphipods, but it is the combination of aging and preserving natural redox gradients that can lower toxicity.
Two risk metrics for CO2storage reservoirs with varying domain size, heterogeneity, and injection rate
Authors: Singh Harpreet , Bromhal Grant S. 
Published: June 2019

AbstractTwo parameters that play the most important role in the appraisal of environmental risk performance at carbon dioxide (CO2) storage sites are the prospective impact of the pore pressure increase and CO2saturation. In this context, this study investigates the spatiotemporal evolution of pressure buildup and CO2saturation as a function of flow region's size, average porosity and permeability, and heterogeneity, as well as the injection rate and total volume of injected fluid. The practical importance of this study is to investigate the factors that affect the extent of pressure buildup and areal extent of CO2plume both during and after injection, which will impact risk assessment as well as influence effective monitoring operations. This study pursues the above objective using two risk metrics that are based on numerical simulations and illustrated using representative models of three realistic storage sites with varying volumetric storage potential and geological settings, all with open geologic systems. The two metrics (the spatiotemporal extent of pressure buildup and CO2saturation plume, respectively) used in this study are able to capture the geologic (structural and petrophysical) and operational complexities that cannot be incorporated into analytical or semianalytical solutions. The results of this study suggest that in addition to the average permeability, the areal extent of the pressure buildup during the injection period is strongly related to the injection rate, whereas the postinjection period may be more strongly influenced by the reservoir heterogeneity. The areal extent of the saturation plume during active injection is highly correlated to the mass of injected fluid, and the postinjection behavior is impacted by the shape of the reservoir–seal interface. These findings are consistent with other recent studies by the National Risk Assessment Partnership (NRAP) on characteristic reservoir behavior. The results have been used to generate pressure and saturation plume profiles (over time) that can be used to support risk‐based decision making. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd.
Insight into the biochemical characterization of phytocystatin from Glycine max and its interaction with Cd+2 and Ni+2
Authors: Siddiqui Sharmin , Siddiqui Mohd. Faizan , Khan Shumaila , Bano Bilqees 
Published: June 2019

AbstractPhytocystatins are cysteine proteinase inhibitors ubiquitously present in plants and animals. They are known to carry out various significant physiological functions and also maintain the balance of protease‐antiprotease activity. In the present disquisition, a phytocystatin after preliminary treatment has been isolated and purified to homogeneity from soybean (Glycine max) by a simple two‐step stratagem using ammonium sulfate fractionation and gel filtration chromatography performed on Sephacryl S‐100‐HR. Soybean phytocystatin (SBPC) was purified with a fold purification of 635 and percent yield of 77.6%. A single band was observed on native gel electrophoresis confirming the homogeneity of the purified SBPC. The molecular weight of SBPC was found to be 19.05 kDa as determined by SDS‐PAGE. The SBPC was found to be devoid of carbohydrate moieties and sulfhydryl group content. The binding stoichiometry of SBPC‐papain interaction was determined by isothermal calorimetry suggesting 1:1 complex, and the value of binding constant (K) was found to be 2.78 × 105 M−1 The affinity of binding (Kd) value obtained through ITC was 3.59 × 10−6 M. The purified SBPC was found to be stable in the pH range of 3 to 7 and is thermostable up to 50°C. The UV‐visible and fluorescence studies showed significant changes in the conformation upon the formation of the SBPC‐papain complex. Furthermore, fluorescence spectroscopy, ANS binding, and caseinolytic activity assay were conducted out to explore the effect of metal ions on SBPC which showed that there was a loss in the inhibitory activity along with conformational changes of SBPC upon complex formation with Cd+2 and Ni+2.
Risk Factors of Ambulatory Central Line–Associated Bloodstream Infection in Pediatric Short Bowel Syndrome
Authors: Seddik Talal B. , Tian Lu , Nespor Colleen , Kerner John , Maldonado Yvonne , Gans Hayley 
Published: June 2019

AbstractBackgroundChildren with short bowel syndrome (SBS) receiving home parenteral nutrition (HPN) are predisposed to ambulatory central line–associated bloodstream infection (A‐CLABSI). Data describing risk factors of this infection in children are limited.MethodsRetrospective cohort, single‐center, case‐crossover study of children ≤18 years old with SBS receiving HPN from January 2012 to December 2016. Univariate and multivariate mixed effect Poisson regression identified the relative risk (RR) of A‐CLABSI with proposed risk factors.ResultsThirty‐five children were identified; median follow‐up was 30 months. A‐CLABSI rate was 4.2 per 1000 central line (CL) days. Univariate analysis identified younger age (RR: 0.92 per 12‐month increase [95% confidence interval {CI}: 0.85‐0.99; P = 0.036]), shorter small intestine length (RR: 0.96 per 10‐cm increase [95% CI: 0.92‐0.99; P = 0.008]), lower citrulline level (RR: 0.86 per 5‐nmol/mL increase [95% CI: 0.75‐0.99; P = 0.036]), and recent CL break (RR: 1.55 [95% CI: 1.06‐2.28; P = 0.024]) as risk factors for A‐CLABSI. Multivariate analysis showed increased A‐CLABSI with clinical diagnosis of small intestine bacterial overgrowth (SIBO) (RR: 1.87 [95% CI: 1.1‐3.17; P = 0.021]) and CL breaks (RR: 1.49 [95% CI: 1‐2.22; P = 0.024]).ConclusionsFactors influencing gut integrity increase A‐CLABSI rate, supporting translocation as an important mechanism and target for prevention. Clinical diagnosis of SIBO increases A‐CLABSI rate, but whether dysbiosis or diarrhea is responsible is an area for future research. CL maintenance is crucial, and prevention of breaks would likely decrease A‐CLABSI rate.