bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between patient attachment to a regular doctor and self‐perceived unmet health care needs in Canada: A population‐based analysis of the 2013 to 2014 Canadian community health surveys
Authors: Awe Oluwakemi A. , Okpalauwaekwe Udoka , Lawal Adegboyega K. , Ilesanmi Marcus M. , Feng Cindy , Farag Marwa 
Published: August 2018

SummaryBackgroundAlthough Canada operates a universal health care insurance system, equitable access to required health care services when needed still poses a challenge for some. The aim of this study was to examine the relationship between patient attachment to a family physician and self‐perceived unmet health care needs (UHN) in Canada, after adjusting for predisposing, enabling, and need factors of the behavioral model of health services use.MethodsThis cross‐sectional study used data from the Canadian Community Health Surveys, cycle 2013 to 2014. A sample of 58 462 individuals aged 12 years and over was analyzed. Logistic regression models were used to examine the relationship between patient attachment and self‐perceived UHN.ResultsAn estimated 10.41% of the Canadian population 12 years and older reported having UHN in the previous year. Among people with self‐perceived UHN, there was significantly greater likelihood of unattachment to a family physician—no regular doctor or having a regular site of care, being younger, being female, being divorced, separated or widowed, having higher education, having lower income, having poorer perceived physical or mental health, having a weak sense of community belonging, having at least one chronic condition, and having greater activity limitations.ConclusionOngoing public discourses on improving primary health care performance and reducing the burden of UHN in Canada should prioritize efforts that promote and facilitate the use of a regular family physician.
Correlation between HDAC4 enhancer DNA methylation and mRNA expression during palatal fusion induced by all‐trans retinoic acid
Authors: Shu Xuan , Cheng Hongqiu , Shu Shenyou , Tang Shijie , Li Ke , Dong Zejun 
Published: August 2018

AbstractEpithelial‐mesenchymal transformation of the medial edge epithelium is the most crucial process in embryonic palatal fusion. This study aimed to explore the relationship and potential mechanism between enhancer DNA methylation and mRNA expression of histone deacetylase 4 (HDAC4) during palatal fusion induced by maternal exposure to all‐trans retinoic acid (ATRA). Pregnant mice were administered ATRA (70 mg/kg) by gavage at embryonic gestation day 10.5 (E10.5) to establish a cleft palate (CP) model in C57BL/6J mice. Control groups were given an equivalent volume of corn oil. Pregnant mice were dissected at E14.5 (n = 6) to obtain embryonic palates. HDAC4 enhancer DNA methylation data were obtained from a previous MethylRAD‐seq. Methylation‐specific polymerase chain reaction (MSP) and real‐time quantitative PCR were used to quantify enhancer methylation and the mRNA expression level of HDAC4. Enhancer DNA methylation at a non‐CpG site within the HDAC4 gene was hyper‐methylated at E14.5 (P: 0.011, log2FC:1.67). The MSP results indicated a similar trend, in agreement with the MethylRAD‐seq results. The change in the HDAC4 expression level was negatively correlated with its enhancer DNA methylation level, at the non‐CpG site, during palatal fusion induced by ATRA. Enhancer DNA methylation of HDAC4 might play an important regulatory role during palatogenesis, especially in embryonic palatal fusion at E 14.5, and may facilitate the development of novel epigenetic biomarkers in the treatment of CP.
Structural basis for drug resistance mechanisms against anaplastic lymphoma kinase
Authors: Goyal Sukriti , Jamal Salma , Shanker Asheesh , Grover Abhinav 
Published: August 2018

AbstractDrug resistance to anaplastic lymphoma kinase (ALK) inhibitors (crizotinib and ceritinib) is caused by mutation in the region encoding kinase domain of ALK. Compounds with potential ability to inhibit all strains of ALK are a solution to tackle the problem of drug resistance. In this study, we delineated positions of residues possessing the ability to make ALK drug resistant upon mutation by assessing them using five parameters (conservation index, binding‐site root‐mean‐square deviation, protein structure stability, change in ATP, and drug‐binding affinity). Four residual positions (Leu 1122, Thr 1151, Phe 1245, and Gly 1269) were ascertained. This study will be beneficial for designing drugs with better proficiency against ALK and the issues of drug resistance. This study can be taken as a pipeline for investigating drug‐resistant mutations in other diseases as well.
Ferulic acid inhibits interleukin 17‐dependent expression of nodal pathogenic mediators in fibroblast‐like synoviocytes of rheumatoid arthritis
Authors: Ganesan Ramamoorthi , Rasool Mahaboobkhan 
Published: August 2018

AbstractInterleukin 17 (IL‐17), a proinflammatory cytokine produced by T helper (Th) 17 cells, potentially controls fibroblast‐like synoviocytes (FLS)‐mediated disease activity of rheumatoid arthritis (RA) via IL‐17/ IL‐17 receptor type A (IL‐17RA)/signal transducer and activator of transcription 3 (STAT‐3) signaling cascade. This has suggested that targeting IL‐17 signaling could serve as an important strategy to treat FLS‐mediated RA progression. Ferulic acid (FA), a key polyphenol, attenuates the development of gouty arthritis and cancer through its anti‐inflammatory effects, but its therapeutic efficiency on IL‐17 signaling in FLS‐mediated RA pathogenesis remains unknown. In the current study, FA markedly inhibited the IL‐17‐mediated expression of its specific transmembrane receptor IL‐17RA in FLS isolated from adjuvant‐induced arthritis (AA) rats. Importantly, FA dramatically suppressed the IL‐17‐mediated expression of toll‐like receptor 3 (TLR‐3), cysteine‐rich angiogenic inducer 61 (Cyr61), IL‐23, granulocyte‐macrophage colony stimulating factor (GM‐CSF) in AA‐FLS via the inhibition of IL‐17/IL‐17RA/STAT‐3 signaling cascade. In addition, FA significantly decreased the formation of osteoclast cells and bone resorption potential in a coculture system consisting of IL‐17 treated AA‐FLS and rat bone marrow derived monocytes/macrophages. Furthermore, FA remarkably inhibited the IL‐17‐mediated expression of receptor activator of nuclear factor κ‐Β ligand (RANKL) and increased the expression of osteoprotegerin (OPG) in AA‐FLS via the regulation of IL‐17/IL‐17RA/STAT‐3 signaling cascade. The therapeutic efficiency of FA on IL‐17 signaling was further confirmed by knockdown of IL‐17RA using small interfering RNA or blocking of STAT‐3 activation with S3I‐201. The molecular docking analysis revealed that FA manifests significant ligand efficiency toward IL‐17RA, STAT‐3, IL‐23, and RANKL proteins. This study provides new evidence that FA can be used as a potential therapeutic agent for inhibiting IL‐17‐mediated disease severity and bone erosion in RA.
The role of community psychology in Christian community development
Authors: Eccleston Sara M. Perisho , Perkins Douglas D. 
Published: August 2018

AbstractThis project examines the connections between community psychology and faith‐based community development. We investigate whether and how 4 major principles of community psychology—neighboring, sense of community, empowerment, and citizen participation—are found in the theory and philosophy of practice of the Christian Community Development Association (CCDA), a national faith‐based community development network. We employ content analysis of four official CCDA publications to identify whether and how these four principles are embedded in the organization's espoused principles of practice. Our findings are as follows: (a) All four principles are found within CCDA theory and philosophy of practice, with the greatest emphasis on neighboring and sense of community and a less robust application of empowerment and citizen participation; (b) CCDA primarily focuses on the individual‐level impact of these principles; and (c) CCDA Bases their application of these principles in Christian scripture and tradition. Our results indicate that the field could be strengthened by examining religious approaches to these principles and considering how organizations engage these concepts in both the theory and the practice. Additionally, faith‐based organizations may foster a more effective application of these concepts in their social change efforts by partnering with community researchers and practitioners.
Transposition of the great arteries: When echocardiography does not match the clinical picture
Authors: Dhillon Gurpreet S. , Fernandes Caraciolo J. , Khan Asra , Heinle Jeffrey S. , Lopez Keila N. 
Published: August 2018

AbstractAortopulmonary window (APW) is a rare form of congenital heart disease seen in isolation or with complex cardiac lesions. APW has been associated with other cardiac defects such as interrupted aortic arch and Tetralogy of Fallot, but few cases have been reported of APW associated with transposition of the great arteries (TGA). In a newborn with TGA and intact ventricular septum, diagnosis of APW requires a high index of suspicion. This article reviews the literature on TGA with APW and illustrates the importance of additional evaluation in neonates with TGA when oxygen saturation and PaO2 do not match predicted clinical values.
Note on list star edge‐coloring of subcubic graphs
Authors: Lužar Borut , Mockovčiaková Martina , Soták Roman 
Published: August 2018

AbstractA star edge‐coloring of a graph is a proper edge‐coloring without bichromatic paths and cycles of length four. In this paper, we consider the list version of this coloring and prove that the list star chromatic index of every subcubic graph is at most 7, answering the question of Dvořák et al (J Graph Theory, 72 (2013), 313‐326).
Defining parameters of specificity for bioluminescent optogenetic activation of neurons using in vitro multi electrode arrays (MEA)
Authors: Prakash Mansi , Medendorp William E. , Hochgeschwender Ute 
Published: August 2018

AbstractIn Bioluminescent Optogenetics (BL‐OG) a biological, rather than a physical, light source is used to activate light‐sensing opsins, such as channelrhodopsins or pumps. This is commonly achieved by utilizing a luminopsin (LMO), a fusion protein of a light‐emitting luciferase tethered to a light‐sensing opsin. Light of the wavelength matching the activation peak of the opsin is emitted by the luciferase upon application of its small molecule luciferin, resulting in activation of the fused opsin and subsequent effects on membrane potential. Using optimized protocols for culturing, transforming, and testing primary neurons in multi electrode arrays, we systematically defined parameters under which changes in neuronal activity are specific to bioluminescent activation of opsins, rather than due to off‐target effects of either the luciferin or its solvent on neurons directly, or on opsins directly. We further tested if there is a direct effect of bioluminescence on neurons. Critical for assuring specific BL‐OG effects are testing the concentration and formulation of the luciferin against proper controls, including testing effects of vehicle on LMO expressing and of luciferin on nonLMO expressing targets.
Variability and trends in record air temperature events of Turkey and their associations with atmospheric oscillations and anomalous circulation patterns
Authors: Türkeş Murat , Erlat Ecmel 
Published: August 2018

The study investigated temporal variations of record and absolute maximum and minimum air temperatures (Tmax and Tmin, respectively), and surface and upper atmospheric conditions in years, in which the highest number of events were observed at 90 stations of Turkey during period of 1950–2015. The results have shown that annual frequency of record Tmin events revealed an evident decreasing since mid‐1980s while frequency of Tmax events increased particularly starting from the year of 2000. Half of record Tmax events were recorded during 2000–2015 period. On the other hand, daily absolute Tmax occurred mostly in 2000, whereas daily absolute Tmin events were mostly recorded in 1953.With respect to linkages to atmospheric teleconnection patterns, statistically significant negative relationships were detected between numbers of Turkey's record Tmax events and the North Atlantic Oscillation Index (NAOI) in July, December, and annually, and between the Arctic Oscillation Index (AOI) and the record Tmax events in winter months. The North Sea–Caspian Pattern Index (NCPI) had a significantly strong negative relationship with record‐breaking Tmax events in February, March, August, October, and annually. However, statistically significant relationships found between the NCPI and record Tmin events in November, January, and annually are different from other atmospheric oscillations.Well‐defined synoptic‐scale surface and upper atmospheric circulation anomalies closely controlled occurrence and nature/magnitude of highest number of record‐breaking Tmax events in Turkey by carrying frequently the tropical origin weather systems and continental tropical warm/hot air masses towards Turkey and her surroundings. On the other hands, record‐breaking Tmin events detected were related closely with the patterns of surface and upper atmospheric circulation anomalies carrying frequently polar and subpolar or mid‐latitudinal weather systems with the continental polar air masses mostly cold and stable to Turkey and her surrounding regions.
Near‐field enhancements and surface plasmon polaritons with multifunctional oxide thin films
Authors: Dab Chahinez , Thomas Reji , Hajlaoui Thameur , Ruediger Andreas 
Published: August 2018

AbstractThe thickness of multifunctional oxide films directly affects the localized enhancement of the electromagnetic field and hence is a key factor for realizing novel photonic or optoelectronic devices. To this end, we theoretically exploit the principle of tip‐enhanced Raman spectroscopy on the surface of multifunctional oxide films (bismuth ferrite and barium titanate) on a gold substrate. An electromagnetic field enhancement of up to 104 in close proximity of the tip apex was obtained. The good agreement between simulation and experiment of spatial resolution of the tip supports the validity of our model. By investigation of surface plasmon polaritons that propagates at the interface between the multifunctional film and the gold substrate, we demonstrated a thickness dependent propagation length and amplitude. This study indicates a critical thickness of the films above which neither a considerable near‐field nor a tip‐mediated excitation are observed. This confirms the validity, promise, and power of tip‐enhanced Raman spectroscopy technique for the analysis of various electro‐optic materials.
Recent applications of on‐line supercritical fluid extraction coupled to advanced analytical techniques for compounds extraction and identification
Authors: Sánchez‐Camargo Andrea del Pilar , Parada‐Alonso Fabián , Ibáñez Elena , Cifuentes Alejandro 
Published: August 2018

AbstractIn a previous review (Sánchez‐Camargo et al., J. Sep. Sci. 40 (2017) 213–227), we discussed the application of on‐line supercritical fluid extraction coupled to chromatographic techniques. This review includes an update of the most recent publications (from January 2016 till June 2018) on this topic, which employs advanced analytical techniques for extracting and identifying valuable analytes. Supercritical fluid extraction has been widely recognized as a green sample preparation technique, because it is efficient, environmentally friendly, powerful, and faster, offering the possibility of direct coupling to analytical instrumental techniques. Among those techniques, supercritical fluid chromatography has experienced an innovative progression in the last 10 years, and the most recent applications of supercritical fluid extraction are coupled to this advanced analytical tool. The general principles, both methodological and instrumental of on‐line supercritical fluid extraction coupled to supercritical fluid chromatography are described here. Besides, applications of the mentioned coupling for analysing biological fluids, food, soil, and botanical samples are also presented and discussed. Finally, a brief description about the very recent on‐line coupling of supercritical fluid extraction to ion mobility spectrometry is presented, as well as concluding remarks about the importance of using these coupled techniques in the near future.
Collagen/Heparin Bi‐Affinity Multilayer Modified Collagen Scaffolds for Controlled bFGF Release to Improve Angiogenesis In Vivo
Authors: Hao Wangping , Han Jie , Chu Yun , Huang Lei , Zhuang Yan , Sun Jie , Li Xiaoran , Zhao Yannan , Chen Yanyan , Dai Jianwu 
Published: August 2018

AbstractBasic fibroblast growth factor (bFGF) is an important protein for wound healing and angiogenesis in tissue engineering, but the lack of a viable delivery system hampers its clinical application. This study aims to maintain the long‐term controlled release of bFGF by utilizing a collagen/heparin bi‐affinity multilayer delivery system (CHBMDS), which is fabricated by the alternate deposition of negatively charged heparin, positively charged collagen, and CBD‐bFGF (a collagen‐binding domain [CBD] was fused into the native bFGF) via specific or electrostatic interaction. The results show that CHBMDS not only support localized and prolonged release of CBD‐bFGF(over 35 days) but also lead to enhanced angiogenesis (higher density and larger diameter (≈70 µm) of newly formed blood vessels in subcutaneous tissue of SD rat after 5 weeks). This system could act as a versatile approach for bFGF delivery and further improve therapeutic efficacy for injured tissues.
Restricted thresholding: recovering smoothness and preserving edges
Authors: Vera Daniel 
Published: August 2018

AbstractRestricted non linear approximation is a generalization of the N‐term approximation in which a measure on the index set of the approximants controls the type, instead of the number, of elements in the approximation. Thresholding is a well‐known type of non linear approximation. We relate a generalized upper and lower Temlyakov property with the decreasing rate of the thresholding approximation. This relation is in the form of a characterization through some general discrete Lorentz spaces. Thus, not only we recover some results in the literature but find new ones. As an application of these results, we compress and reduce noise of some images with wavelets and shearlets and show, at least empirically, that the L2‐norm is not necessarily the best norm to measure the approximation error.
Genome editing in induced pluripotent stem cells rescues TAF1 levels in X‐linked dystonia‐parkinsonism
Authors: Rakovic Aleksandar , Domingo Aloysius , Grütz Karen , Kulikovskaja Leonora , Capetian Philipp , Cowley Sally A. , Lenz Insa , Brüggemann Norbert , Rosales Raymond , Jamora Dominic , Rolfs Arndt , Seibler Philip , Westenberger Ana , König Inke , Klein Christine 
Published: August 2018

AbstractBackground: The most likely genetic cause of X‐linked dystonia‐parkinsonism, a neurodegenerative movement disorder endemic to the Philippines, is a 2672‐bp‐long retrotransposon insertion in intron 32 of the TAF1 gene. The objectives of this study were to investigate whether (1) TAF1 expression is altered in induced pluripotent stem cells and differentiated neuronal models and (2) excision of the retrotransposon insertion restores normal TAF1 expression.Methods: Expression of TAF1 and its neuronal isoform were determined in induced pluripotent stem cells and in induced pluripotent stem cell‐derived cortical neurons and spiny projection neurons using quantitative PCR. Genome editing‐based excision of the retrotransposon insertion was performed on induced pluripotent stem cells from 3 X‐linked dystonia‐parkinsonism patients. Edited and unedited induced pluripotent stem cells from X‐linked dystonia‐parkinsonism patients and controls were differentiated into cortical neurons and spiny projection neurons, and TAF1 expression was compared across groups.Results: TAF1 was reduced in patient‐derived induced pluripotent stem cells (P < 0.05) and spiny projection neurons (P < 0.01). After genome editing, we observed higher TAF1 expression in edited compared with unedited induced pluripotent stem cells (P < 0.0001). In edited spiny projection neurons, TAF1 expression was also increased, but did not reach statistical significance. No expression differences were observed in cortical neurons.Conclusions: (1) TAF1 reduction in X‐linked dystonia‐parkinsonism is likely due to the retrotransposon insertion and is recapitulated in induced pluripotent stem cells and differentiated spiny projection neurons. (2) TAF1 reduction is a tractable molecular phenotype of X‐linked dystonia‐parkinsonism that can be driven by excision of the retrotransposon insertion. (3) Successful rescue of the molecular phenotype in an endogenous, genome‐edited model serves as a proof of principle that may successfully be transferred to other inherited neurodegenerative diseases. © 2018 International Parkinson and Movement Disorder Society
Gamma‐aminobutyric acid edited echo‐planar spectroscopic imaging (EPSI) with MEGA‐sLASER at 7T
Authors: Magnusson Peter O. , Boer Vincent O. , Marsman Anouk , Paulson Olaf B. , Hanson Lars G. , Petersen Esben T. 
Published: August 2018

PurposeFor rapid spatial mapping of gamma‐aminobutyric acid (GABA) at the increased sensitivity and spectral separation for ultra‐high magnetic field strength (7 tesla [T]), an accelerated edited magnetic resonance spectroscopic imaging technique was developed and optimized for the human brain at 7 T.MethodsA MEGA‐sLASER sequence was used for GABA editing and volume selection to maximize editing efficiency and minimize chemical shift displacement errors. To accommodate the high bandwidth requirements at 7 T, a single‐shot echo planar readout was used for rapid simultaneous encoding of the temporal dimension and 1 spatial. B0 and B1 field aspects specific for 7 T were studied together with correction procedures, and feasibility of the EPSI MEGA‐sLASER technique was tested in vivo in 5 healthy subjects.ResultsLocalized edited spectra could be measured in all subjects giving spatial GABA signal distributions over a central brain region, having 45‐ to 50‐Hz spatial intervoxel B0 field variations and up to 30% B1 field deviations. MEGA editing was found unaffected by the B0 inhomogeneities for the optimized sequence. The correction procedures reduced effects of intervoxel B0 inhomogeneities, corrected for spatial editing efficiency variations, and compensated for GABA resonance phase and frequency shifts from subtle motion and acquisition instabilities. The optimized oscillating echo‐planar gradient scheme permitted full spectral acquisition at 7 T and exhibited minimal spectral‐spatial ghosting effects for the selected brain region.ConclusionThe EPSI MEGA‐sLASER technique was shown to provide time‐efficient mapping of regional variations in cerebral GABA in a central volume of interest with spatial B1 and B0 field variations typical for 7 T.