bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antithrombin- und Antiplasminwirkung von 4-Amidinophenylbrenztraubensäure (APPA) in vivo
Authors: Markwardt F , Klöcking H.-P , Nowak G 
Published: June 2018

ZusammenfassungEs wurde die Antithrombin- und Antiplasmin Wirkung der 4-Amidinophenylbrenztraubensäure (APPA) an Kaninchen untersucht. Dabei hat sich gezeigt, daß die in biochemischen Untersuchungen als kompetitiver Hemmstoff der Gerinnungsfermente wirkende Verbindung auch in vivo antikoagulierende und antifibrinolytische Wirkungen entfaltet, die sich sowohl nach intravenöser als auch nach oraler Gabe des Inhibitors messend verfolgen lassen.Die Wirkung von Thrombininfusionen oder Streptokinaseinfusionen wurde bei Gegenwart des Inhibitors im Blut unterbunden.Die Resorptionsquote der APPA nach oraler Applikation beträgt 45% und die Halbwertzeit 100 min. Ein Teil der applizierten Inhibitormenge wird in unveränderter Form mit dem Urin ausgeschieden.APPA stellt ein pharmakologisches Modell für eine neue Klasse von Stoffen zur medikamentösen Beeinflussung der Blutgerinnungs- und Fibrinolyse Vorgänge dar.
Human Prothrombin
Authors: Derleth Th. R , Penner J. A 
Published: June 2018

SummaryA method is presented for the chromatographic separation of human prothrombin from variable volumes of plasma. The procedure is simple, consisting of chromatography on ECTEOLA which provides a potent product containing factor IX and X activity sufficient for therapeutic use. Additional chromatography on DEAE or Sephadex G200 will produce a prothrombin of high specific activity which appears homogeneous by ultracentrifugation and electrophoresis on cellulose acetate, although multiple protein fractions can be identified on starch gel electrophoresis.Human prothrombin prepared by this method migrates as an alpha 2-globulin and does not appear to be altered electrophoretically or antigenically from its original state in the plasma. The characteristics of human prothrombin obtained from patients with deficiencies of factor VII or IX do not differ from normal.
Immunological Identification of Three Urokinases
Authors: Day Eugene D. , Ball Anne P. 
Published: June 2018

SummaryA third urokinase has been detected by immunochemical tests in two clinical preparations of urokinase, one from Abbott and one from Sterling-Winthrop. It is component V in a series of components found in Sterling-Winthrop clinical urokinase. Components I and II are confined to Sterling-Winthrop urokinase whereas components III, IV, and V are present also in Abbott urokinase. Evidence suggests but does not prove that V is the SI component of Abbott; that IV is the S2 component of Abbott as well as the crystalline component of Sterling -Winthrop ; and that III is a higher molecular weight species of urokinase, unrelated to either IV or V, which may correspond to the 104,000 MW uroprotein of Doleschel and Auerswald. It is proposed that III be referred to as S3.
A Simple Test for Quantitation of Platelet Adhesion to Glass
Authors: Mason R. G , Gilkey J. M 
Published: June 2018

SummaryA simple method for quantitating pletelet adhesiveness to glass is described. The use of minimally manipulated native whole blood and direct counting of adherent platelets are principal features of the method. Normal values for platelet adhesiveness to glass both in the absence and presence of added ADP are given. Without ADP, abnormally low platelet adhesiveness values were observed in afibrinogenemia, thrombasthenia, uremia, homocystinuria and in some patients with von Willebrand’s disease or Hageman trait. Under the same test condition, abnormally elevated values were found in some patients with von Willebrand’s disease or diabetes mellitus. With added ADP, which induced aggregation of some platelets in the blood, abnormally low platelet adhesiveness values were observed in thrombasthenia and one diabetic patient, while abnormally high values were found in von Willebrand’s disease, and in one patient with homocystinuria. The test for platelet adhesiveness performed with added ADP may have diagnostic value for patients with von Willebrand’s disease.
Untersuchungen über die durch Kollagen ausgelöste 5-HT-Freisetzung aus Blutplättchen
Authors: Barthel W , Markwardt F 
Published: June 2018

ZusammenfassungBei Einwirkung von Sehnenextrakt auf Kaninchenblutplättchen in Tyrodelösung kommt es zu einer Freisetzung des gespeicherten 5-HT. Die 5-HT-Freisetzung ist abhängig von der zugesetzten Sehnenextraktmenge und läuft mit großer Geschwindigkeit ab. Glukose- und Kalziumentzug sowie die Vorbehandlung des Sehnenextrakte mit Kollagenase hemmen die Freisetzungsreaktion. Papaverin, NEM und das Isochinolinderivat B30 hemmen die durch Sehnenextrakt ausgelöste 5-HT-Freisetzung in dosisabhängiger Weise. Adenosin und Azetylsalizylsäure hatten unter den verwendeten Versuchsbedingungen keinen Einfluß auf die 5-HT-Freisetzung.
Morphologische, biochemische und eiweißanalytische Untersuchungen bei einem Fall von Riesenplättchen-Thrombozytopathie mit Bence-Jones-Proteinurie
Authors: Schneider W , Hübner G , Scheurlen P. G , Joist J , Kübler W , Lechler E , Hellriegel K. P , Gross R 
Published: June 2018

ZusammenfassungDie Ergebnisse feinstruktureller, biochemischer, immunologischer und chromosomenanalytischer Untersuchungen bei einem Patienten mit Riesenplättchenthrombozytopathie werden berichtet und Übereinstimmungen und Unterschiede zu den Befunden an anderen genetisch bedingten, thrombozytopenischen Thrombozytopathien aufgezeigt.
Über den Reaktionsmechanismus der Veränderungen von Kaninchenfibrinogen durch Kobalt(II)-Ionen
Authors: Taube Chr , Fiedler H 
Published: June 2018

ZusammenfassungBei einigen Tieren und beim Menschen führen Kobalt(II)-Ionen durch Änderung der Konformation des Fibrinogenmoleküls zu einer Gerinnungsstörung. Die Artspezifität des Fibrinogenmoleküls spielt dabei keine Rolle. Der Effekt ist von der Anwesenheit eines hochmolekularen Plasmafaktors abhängig, der bei einigen Tierarten nicht oder in ungenügender Konzentration vorhanden ist. Kobalt-58 wird an Fibrinogen angelagert, ohne daß es dadurch in jedem Fall zu einer Gerinnungsstörung kommt. Die Inkubation eines Gemisches aus Fibrinogen und defibriniertem, mit Kobalt(II) vorinkubiertem Plasma führt selbst dann zu einer Störung der Gerinnungs-fähigkeit des Fibrinogens, wenn die freien Kobalt(II)-Ionen nach Zugabe des Plasmas als Chelatkomplex gebunden werden.
Activation of the Fibrinolytic Enzyme System in Laboratory Animals and in Man
Authors: Baillie A. J , Sim A. K 
Published: June 2018

SummaryThe activation characteristics of the fibrinolytic system of several species have been evaluated in vitro and compared with the human enzyme system. The cat, dog and baboon would appear to be the most suitable species for further study of fibrinolytic activation. The present findings have been found to parallel the species differences in platelet behaviour which have been reported separately from this laboratory.
Platelet Sensitivity to Aggregation in Normal and Diseased Groups
Authors: Sano T , Boxer M. G. J , Boxer L. A , Yokoyama M 
Published: June 2018

SummaryA method to estimate the sensitivity of platelets to aggregation by ADP has been described. Platelet-rich plasma was obtained from 8% trisodium citrate anticoagulated blood. ADP in trishydroxymethylaminomethane buffered saline was prepared by serial two-fold dilutions in concentrations of 2−1 through 2−18 mg/ml. Fifty μl each of platelet-rich plasma and ADP solution were mixed in a plastic test tube and agitated for 1 min. Aggregation was observed utilizing a light microscope. All procedures were performed at room temperature. The coefficient of variation in one sample was 1.4% or less and the daily variation was slight in healthy male volunteers. An advantage of this method is that it requires only a small amount of blood for the performance of many tests.In 105 healthy Japanese males, the sensitivity of platelets to ADP-induced aggregation was positively correlated with age with a correlation coefficient of 0.824.In the acute stage of thromboembolism of myocardial infarction or stroke, value of platelet sensitivity to ADP-induced aggregation was significantly higher at the 0.05 to 0.01 confidence limits than in age-, and sex-matched controls.
Aggregation of Fibrinogen and Its Degradation Products by Basic Proteins
Authors: Stewart G. J , Niewiarowski S 
Published: June 2018

SummaryFibrinogen and early fibrinogen degradation products (FDP) of plasmin digestion containing Fragment XfP gave highly ordered polymers of limited branching with protamines and synthetic polylysine (molecular weight 175,000). Histones, polylysine of molecular weight 2,500, granulocyte cationic protein and platelet factor 4 formed amorphous precipitates with fibrinogen and early FDP. Fibrin degradation products (fdp) containing complexes of Fragment X° formed highly ordered polymers of extensive branching with these basic proteins.The splitting off of fibrinopeptides from fibrinogen and Fragment XfP was not essential for the formation of highly ordered polymers. However, in the presence of fibrinopeptides, high specificity of polymerizing agents such as basic protein is required. In the absence of fibrinopeptides, fibrin monomer or Fragment X° could polymerize after dissociation from complexes with other degradation products by any of the basic proteins.It has been suggested that the polymerization of fibrinogen and its derivatives by basic proteins may be an important factor contributing to the formation and deposition of fibrin-like material in clinical conditions connected with the destruction of tissues (release of histones), damage of granulocytes (release of lysosomal cationic protein) and intravascular formation of platelet aggregates (release of platelet factor 4).
The Papaveraceae: Benevolence—Opium, Chelidonium and Sanguinaria
Authors: Leupen Alex 
Published: June 2018

AbstractAlex Leupen has worked in the Homeopathic Doctors Centre in Utrecht, the Netherlands, since 1990. Eight colleagues work in this centre, including Jan Scholten. Alex is an experienced teacher (the Netherlands, Belgium, France and Germany) on the underlying systems in the plant families, animal kingdom, sarcodes, nosodes, fungi and the periodic table of the elements pertaining to homeopathic practice. In this column, he shares clinical tips.
Surgical Management of Pulmonary Mucormycosis in Third-Trimester Pregnancy
Authors: Ramirez Joel , Urisman Anatoly , Kukreja Jasleen , Kratz Johannes 
Published: June 2018

Background Pulmonary mucormycosis is a rare fungal infection that carries a high mortality. Given the rarity of this disease, its management has not been well established.
Case Description We report a 36-year-old female presenting with right middle and lower lobe pulmonary mucormycosis during the third trimester of pregnancy. Diagnosis was established using chest computed tomography followed by bronchoalveolar lavage and lung biopsy. Prompt initiation of amphotericin B and right middle and lower lobe lobectomy resulted in maternal survival and fetal viability.
Conclusion This favorable outcome is attributed to extensive communication between treatment teams in addition to comprehensive surgical planning.
Primary Cardiac B-Non-Hodgkin Lymphoma Disguised as a Pacemaker Endocarditis
Authors: Fleißner Felix , Molitoris Ulrich , Rösler Wiebke , Kühn Christian 
Published: June 2018

Background Pacemaker infections rates are high compared with the incidence of primary malignant cardiac tumors. However, they can look alike in diagnostics and patient presentation.
Case Description We hereby report a rare case of a suspected pacemaker endocarditis which in fact turned out to be a primary cardiac B cell lymphoma. The lymphoma was removed surgically.
Conclusion Sometimes we encounter the unexpected. Suboptimal preoperative diagnostics certainly lead to the faulty conclusion of an endocarditis. Nonetheless, even such an enhanced primary cardiac tumor can be resected with good clinical outcome and long-term survival.
Platelet-Rich Plasma for the Treatment of Androgenic Alopecia: A Systematic Review
Authors: Chen Jenny , Justicz Natalie , Lee Linda 
Published: June 2018

AbstractThe use of platelet-rich plasma (PRP) has become increasingly commonplace in facial plastic surgery for the treatment of androgenic alopecia (AGA). However, this treatment remains novel with a range of application techniques and outcomes described in the literature. Herein, the authors systematically review the existing literature on the use and efficacy of PRP for AGA. Systematic review of PubMed, Embase, and Cochrane databases was performed. Case reports were excluded. Twenty-four papers met inclusion criteria for this study: 8 randomized control trials and 16 prospective cohort studies. Twenty-one studies used clinical criteria to diagnose AGA, while three used confirmatory biopsies. PRP was injected with or without the use of a numbing agent, and most studies performed multiple injections (three or more separated by several weeks). Twenty-one studies reported positive outcomes by objective criteria (88%), while three suggested that there was no clinical improvement, although in two of these studies patients still reported increased satisfaction. There were no complications reported other than transient edema/erythema and pain/headache associated with the procedure. The existing literature suggests that PRP is a low-risk intervention to treat AGA associated with good patient satisfaction and objective improvements in outcomes. Further research is needed to optimize preparation and delivery methods as well as standardize measurements of clinical outcomes.
Distally Based Peroneus Brevis Muscle Flap for Large Leg, Ankle, and Foot Defects: Anatomical Finding and Clinical Application
Authors: Abd-Al-Moktader Magdy 
Published: June 2018

Background Peroneus brevis muscle flap is a distinguished, distally based safe flap that can be manipulated to cover small defects in the leg and ankle. For large-sized defects, a more distal, larger flap is required either locally or distantly.
Methods Forty-two distally based peroneus brevis muscle flaps were elevated in 42 patients (30 males and 12 females) with major lower leg, ankle, and proximal foot defects of 6 to 15 cm in length and 6 to 12 cm in width. Anatomical findings were recorded as number, size, and sources of blood supply, entry sites, the lowermost two arterial supplies, internal distribution of blood supply to the muscle, the relationship between external and internal distribution of the blood vessels, the length of the muscle, the entry site of the main artery, and the splitting of the proximal portion of the peroneus brevis muscle to increase its width to sufficiently cover large defects.
Results The anatomical findings suggested that the muscle can be safely extended to cover a large defect in the leg, ankle, or proximal foot. In addition, the longitudinal splitting of the muscle increases its width by up to three times, making it an excellent long-surviving flap to cover a large defect.
Conclusion A distally based peroneus brevis muscle flap has a rich blood supply and safely reaches the proximal foot, with a secure splitting to cover large defects in the leg, ankle, and proximal foot.
Parallel Stenting for Treatment of an In-stent Chronic Total Occlusion after Successful Dissection Reentry Technique
Authors: Koutouzis Michael , Kintis Konstantinos , Kontopodis Eleftherios , Nikitas Georgios , Lazaris Efstathios 
Published: June 2018

AbstractPercutaneous coronary interventions (PCI) for chronic total occlusions (CTO) are the most challenging type of procedure in interventional cardiology and are traditionally associated with increased complexity and reduced procedural success rates. New techniques, such as retrograde approach and dissection reentry technique, offer alternatives in case of traditional antegrade wiring failure. In this paper, we present a successful implantation of a stent parallel to other existing stent in an in-stent CTO (IS-CTO) using dissection reentry technique. The technical details involved and the clues to successful outcome in an individual with in-stent CTO are discussed.
Toxins Are an Excellent Source of Therapeutic Agents against Cardiovascular Diseases
Authors: Koh Cho Yeow , Modahl Cassandra , Kulkarni Namrata , Kini R. Manjunatha 
Published: June 2018

AbstractVenomous and hematophagous animals use their venom or saliva for survival, to obtain food, and for self-defense. Venom and saliva from these animals are cocktails of bioactive molecules primarily composed of proteins and peptides. These molecules are called toxins because they cause unwanted consequences on prey. They exhibit unique, diverse, and specific biological activities that perturb normal physiological processes of their prey and host. However, the potential of toxins as inspirations for the development of therapeutic agents or pharmacological tools has also long been recognized. In addition to their small size, the exquisite selectivity and structural stability of toxins make them attractive as starting molecule in the development of therapeutic and diagnostic agents. Drug discovery and development from venomous and hematophagous animals against cardiovascular diseases have been particularly successful. Some of the notable success include antihypertensive (captopril and enalapril) and antiplatelet agents (tirofiban and eptifibatide), as well as anticoagulants (lepirudin and bivalirudin). Highlighted in this review are many venom or saliva-derived cardiovascular-active proteins and peptides of therapeutic interest, including those that are currently in preclinical stages and those that have been approved by FDA and currently in the market. The authors attempt to summarize their structure, function, mechanism of action, and development with respect to cardiovascular diseases.
Surgery for Triple Pathology of Giant Vestibular Schwannoma Associated with Carotid Artery Stenosis and Intracranial Aneurysm: Case Report
Authors: Sirko Andriy , Dzyak Lyudmila , Romanukha Dmytro , Pilipenko Grigoriy , Cherednychenko Yuri 
Published: June 2018

AbstractHere we report the case of a 56-year-old woman who presented with a highly unusual combination of three pathologies, namely a giant left-sided vestibular schwannoma, severe stenosis of the left internal carotid artery, and a right anterior cerebral artery aneurysm. The chosen approach comprised three consecutive surgical procedures, namely (1) aneurysm embolization, (2) carotid stenosis stenting, and (3) vestibular schwannoma excision. This approach avoided complications and achieved a satisfactory functional outcome. To the best of our knowledge, this combination of three conditions has not been previously reported in the English-language literature.
Economic Analysis of Using Free Fat Graft or Acellular Dermis to Prevent Post-parotidectomy Frey Syndrome
Authors: Svider Peter , Raza Syed , Zuliani Giancarlo , Carron Michael , Shkoukani Mahdi , Hojjat Houmehr 
Published: June 2018

AbstractA decision tree was constructed to determine the incremental cost-effectiveness ratio (ICER) of grafting techniques used to prevent Frey Syndrome. The authors performed a sensitivity analysis to calculate what the probability of preventing Frey Syndrome would have to be and maximum costs associated with using grafting techniques to warrant their use as more “cost-effective” choice than using neither. Decision pathways utilized were uses of (1) free fat graft (FFG), (2) acellular dermis, and (3) no grafting. The probability of developing Frey syndrome and costs were extracted from previous studies to construct the decision tree. The primary effectiveness was the ICER of FFG or acellular dermis to prevent Frey syndrome. The initial outcomes included preventing Frey syndrome (effectiveness = 1) or developing Frey syndrome (effectiveness = 0). Compared with not using a graft, the ICER of using FFG and acellular dermis were $10,628 and $50,813, respectively. Frey syndrome was found in 2.6% of patients postoperatively in FFG group, 9.8% of patients in acellular dermis group, and 30.7% of patients who did not have a graft. The ICER shows absolute dominance of FFG with lower cost and high effectiveness over acellular dermis. This economic evaluation strongly supports the use of FFG over acellular dermis as cost-effective approach for prevention of postparotidectomy Frey syndrome.
Endovascular Stent Graft Exclusion of an Iatrogenic Tracheoinnominate Fistula after Unsuccessful Surgical Repair
Authors: Woerner Andrew , Koo Kevin , Monroe Eric 
Published: June 2018

AbstractA 10-year-old girl with metachromatic leukodystrophy developed a tracheoinnominate artery fistula and massive hemoptysis following tracheostomy, initially requiring open surgical repair. Massive hemoptysis recurred at 1 month due to suspected pericardial patch dehiscence. The authors herein describe the subsequent emergent treatment by stent graft exclusion and review the existing literature surrounding this rare and potentially fatal condition.
Evaluation einer neuropsychologischen Testbatterie an psychiatrischen und psychosomatischen Patienten
Authors: Bartl Helga , Buschert Verena , Zwanzger Peter , Diemer Julia , Brunnauer Alexander 
Published: June 2018

ZusammenfassungNeuropsychologische Untersuchungen sollten ein integraler Bestandteil der klinisch-psychiatrischen Diagnostik sein. Die gängigen Testverfahren wurden bisher jedoch nicht ausreichend an dieser Klientel untersucht. In der vorliegenden Untersuchung wurde eine im klinischen Alltag bewährte neuropsychologische Testbatterie evaluiert. Hierzu wurden Daten von 226 psychisch kranken Patienten anhand von Faktoren- und Regressionsanalysen ausgewertet. Es erwies sich als sinnvoll, drei Faktoren zu extrahieren (Speed, Gedächtnis, Exekutivfunktionen), zu denen sich die Einzeltests plausibel zuordnen ließen. Anhand regressionsanalytischer Berechnungen konnte zudem ein zeitökonomisches Basisassessment aus drei Einzeltests (TAP Alertness, VLMT und Matrizentest) ermittelt werden. Zusammenfassend kann vor dem Hintergrund der Akzeptanz, Ökonomie und faktoriellen Struktur die evaluierte Testbatterie für den Einsatz bei psychiatrischen und psychosomatischen Patienten empfohlen werden.