bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pulsatile hyperglycemia increases insulin secretion but not pancreatic β-cell mass in intrauterine growth-restricted fetal sheep
Authors: Boehmer B. H. , Brown L. D. , Wesolowski S. R. , Hay W. W. , Rozance P. J. 
Published: July 2018

AbstractImpaired β-cell development and insulin secretion are characteristic of intrauterine growth-restricted (IUGR) fetuses. In normally grown late gestation fetal sheep pancreatic β-cell numbers and insulin secretion are increased by 7–10 days of pulsatile hyperglycemia (PHG). Our objective was to determine if IUGR fetal sheep β-cell numbers and insulin secretion could also be increased by PHG or if IUGR fetal β-cells do not have the capacity to respond to PHG. Following chronic placental insufficiency producing IUGR in twin gestation pregnancies (n=7), fetuses were administered a PHG infusion, consisting of 60 min, high rate, pulsed infusions of dextrose three times a day with an additional continuous, low-rate infusion of dextrose to prevent a decrease in glucose concentrations between the pulses or a control saline infusion. PHG fetuses were compared with their twin IUGR fetus, which received a saline infusion for 7 days. The pulsed glucose infusion increased fetal arterial glucose concentrations an average of 83% during the infusion. Following the 7-day infusion, a square-wave fetal hyperglycemic clamp was performed in both groups to measure insulin secretion. The rate of increase in fetal insulin concentrations during the first 20 min of a square-wave hyperglycemic clamp was 44% faster in the PHG fetuses compared with saline fetuses (P<0.05). There were no differences in islet size, the insulin+ area of the pancreas and of the islets, and β-cell mass between groups (P>0.23). Chronic PHG increases early phase insulin secretion in response to acute hyperglycemia, indicating that IUGR fetal β-cells are functionally responsive to chronic PHG.
Diverse Continental Subduction Scenarios Along the Arabia‐Eurasia Collision Zone
Authors: Kaban Mikhail K. , Petrunin Alexey G. , El Khrepy Sami , Al‐Arifi Nassir 
Published: July 2018

AbstractIt is generally accepted that convergence of the Arabian and African plates is a subduction‐dominated process. However, the subduction scenarios are still debatable. Here we present a 3‐D model of the mantle to a depth of 700 km, based on a joint interpretation of the seismic tomography, residual topography, residual mantle gravity field, and seismicity. At the northwestern edge of the collision zone, we only observe partial underthrust of the Eurasian plate under the West Greater Caucasus. We suggest that this is the initial stage of subduction polarity reversal after the break off of the plate formerly subducted northward. To the southeast, counteracting subduction zones are found beneath northwest Zagros in the south and beneath the East Greater Caucasus and Alborz in the north. This scenario is likely the result of highly buoyant and weak blocks of the Lesser Caucasus, Alborz, and northwest Zagros, which are underlain in the south by the Arabian plate and the Scythian plate and South Caspian from the opposite side. Further to the southeast, a delaminated lithospheric slab is observed under southeast Zagros, while the Arabian and Eurasian plates only partially underthrust East Zagros and Kopet Dagh, respectively. In the southern part of the collision zone under Makran, only remnants of the formerly subducted slabs are found below 200 km.
Patterns of Evaporation and Precipitation Drive Global Isotopic Changes in Atmospheric Moisture
Authors: Bailey Adriana , Posmentier Eric , Feng Xiahong 
Published: July 2018

AbstractBecause water isotope ratios respond to phase changes during evaporation (E) and precipitation (P), they are candidate fingerprints of changing atmospheric hydrology. Moreover, through preservation in ice cores and other paleoproxies, they provide important insight into the past. Still, there is disagreement over what specific attributes of hydroclimate variability isotopes reveal. Here we argue that variations in zonal mean isotope ratios of water vapor and precipitation are largely a response to geographically shifting patterns of E and P. Differences in the relative importance of local versus remote changes in these moisture variables explain the apparent distinct isotopic sensitivities to temperature and precipitation amount in high and low latitudes, respectively. Not only does our work provide a unified framework for interpreting water isotopic measurements globally, but it also presents a novel approach for diagnosing water cycle changes in a warmer world.
An Intercomparison of Skill and Overconfidence/Underconfidence of the Wintertime North Atlantic Oscillation in Multimodel Seasonal Forecasts
Authors: Baker L. H. , Shaffrey L. C. , Sutton R. T. , Weisheimer A. , Scaife A. A. 
Published: July 2018

AbstractRecent studies of individual seasonal forecast systems have shown that the wintertime North Atlantic Oscillation (NAO) can be skillfully forecast. However, it has also been suggested that these skillful forecasts tend to be underconfident, meaning that there is too high a proportion of unpredictable noise in the forecasts. We assess the skill and overconfidence/underconfidence of the seasonal forecast systems contributing to the EUROpean Seasonal to Interannual Prediction (EUROSIP) multimodel ensemble system. Five of the seven systems studied have significant skill for forecasting the wintertime NAO at 2‐ to 4‐month lead times. Four of these skillful systems are underconfident for forecasting the NAO. A multimodel ensemble (ensemble size 126 members) is both skillful and clearly underconfident. Underconfidence becomes more pronounced as the ensemble size increases. Certain years in the hindcast period are well forecast by all or most models. This implies that common teleconnections and drivers of the NAO are being captured by the EUROSIP seasonal forecasts.
Enhanced bacterial mutualism through an evolved biofilm phenotype
Authors: Røder Henriette L , Herschend Jakob , Russel Jakob , Andersen Michala F , Madsen Jonas S , Sørensen Søren J , Burmølle Mette 
Published: July 2018

Abstract
Microbial communities primarily consist of multiple species that affect one another’s fitness both directly and indirectly. This study showed that the cocultivation of Paenibacillus amylolyticus and Xanthomonas retroflexus exhibited facultative mutualistic interactions in a static environment, during the course of which a new adapted phenotypic variant of X. retroflexus appeared. Although the emergence of this variant was not directly linked to the presence of P. amylolyticus, its establishment in the coculture enhanced the productivity of both species due to mutations that stimulated biofilm formation. The mutations were detected in genes encoding a diguanylate cyclase predicted to synthesise cyclic-di-GMP. Examinations of the biofilm formed in cocultures of P. amylolyticus and the new variant of X. retroflexus revealed a distinct spatial organisation: P. amylolyticus only resided in biofilms in association with X. retroflexus and occupied the outer layers. The X. retroflexus variant therefore facilitated increased P. amylolyticus growth as it produced more biofilm biomass. The increase in X. retroflexus biomass was thus not at the expense of P. amylolyticus, demonstrating that interspecies interactions can shape diversification in a mutualistic coculture and reinforce these interactions, ultimately resulting in enhanced communal performance.
Microorganisms facilitate uptake of dissolved organic nitrogen by seagrass leaves
Authors: Tarquinio Flavia , Bourgoure Jeremy , Koenders Annette , Laverock Bonnie , Säwström Christin , Hyndes Glenn A 
Published: July 2018

Abstract
Microorganisms play a critical role in nitrogen cycling by mineralising dissolved organic nitrogen (DON) compounds into bioavailable inorganic forms (DIN). Although DIN is crucial for seagrass growth, the hypothesis that seagrass leaf associated-microorganisms could convert DON to forms available for plant uptake has never been tested. We conducted a laboratory-based experiment in which seagrass (Posidonia sinuosa) leaves were incubated with 15N-amino acids (aa), with and without associated microorganisms. Samples were collected after 0.5, 2, 6 and 12 h. Both bulk stable isotope and nanoscale secondary ion mass spectrometry (NanoSIMS) analysis showed high accumulation of 15N within seagrass leaf tissues with an associated microbiota, but not in plants devoid of microorganisms. These results significantly change our understanding of the mechanisms of seagrass nitrogen use and provide evidence that seagrass microbiota increase nitrogen availability for uptake by seagrass leaves by mineralising aa, thus enhancing growth and productivity of these important coastal ecosystems.
Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs by orders of magnitude
Authors: Brenner Jacob S. , Pan Daniel C. , Myerson Jacob W. , Marcos-Contreras Oscar A. , Villa Carlos H. , Patel Priyal , Hekierski Hugh , Chatterjee Shampa , Tao Jian-Qin , Parhiz Hamideh , Bhamidipati Kartik , Uhler Thomas G. , Hood Elizabeth D. , Kiseleva Raisa Yu. , Shuvaev Vladimir S. , Shuvaeva Tea , Khoshnejad Makan , Johnston Ian , Gregory Jason V. , Lahann Joerg , Wang Tao , Cantu Edward , Armstead William M. , Mitragotri Samir , Muzykantov Vladimir 
Published: July 2018

AbstractDrug delivery by nanocarriers (NCs) has long been stymied by dominant liver uptake and limited target organ deposition, even when NCs are targeted using affinity moieties. Here we report a universal solution: red blood cell (RBC)-hitchhiking (RH), in which NCs adsorbed onto the RBCs transfer from RBCs to the first organ downstream of the intravascular injection. RH improves delivery for a wide range of NCs and even viral vectors. For example, RH injected intravenously increases liposome uptake in the first downstream organ, lungs, by ~40-fold compared with free NCs. Intra-carotid artery injection of RH NCs delivers >10% of the injected NC dose to the brain, ~10× higher than that achieved with affinity moieties. Further, RH works in mice, pigs, and ex vivo human lungs without causing RBC or end-organ toxicities. Thus, RH is a clinically translatable platform technology poised to augment drug delivery in acute lung disease, stroke, and several other diseases.
Graphene’s nonlinear-optical physics revealed through exponentially growing self-phase modulation
Authors: Vermeulen Nathalie , Castelló-Lurbe David , Khoder Mulham , Pasternak Iwona , Krajewska Aleksandra , Ciuk Tymoteusz , Strupinski Wlodek , Cheng JinLuo , Thienpont Hugo , Van Erps Jürgen 
Published: July 2018

AbstractGraphene is considered a record-performance nonlinear-optical material on the basis of numerous experiments. The observed strong nonlinear response ascribed to the refractive part of graphene’s electronic third-order susceptibility χ(3) cannot, however, be explained using the relatively modest χ(3) value theoretically predicted for the 2D material. Here we solve this long-standing paradox and demonstrate that, rather than χ(3)-based refraction, a complex phenomenon which we call saturable photoexcited-carrier refraction is at the heart of nonlinear-optical interactions in graphene such as self-phase modulation. Saturable photoexcited-carrier refraction is found to enable self-phase modulation of picosecond optical pulses with exponential-like bandwidth growth along graphene-covered waveguides. Our theory allows explanation of these extraordinary experimental results both qualitatively and quantitatively. It also supports the graphene nonlinearities measured in previous self-phase modulation and self-(de)focusing (Z-scan) experiments. This work signifies a paradigm shift in the understanding of 2D-material nonlinearities and finally enables their full exploitation in next-generation nonlinear-optical devices.
Colletotrichum Species Causing Anthracnose of Rubber Trees in China
Authors: Liu Xianbao , Li Boxun , Cai Jimiao , Zheng Xiaolan , Feng Yanli , Huang Guixiu 
Published: July 2018

AbstractAnthracnose caused by Colletotrichum is one of the most severe diseases of Hevea brasiliensis. However, research on the diversity and geographical distribution of Colletotrichum remains limited in China. In this study, we investigated the phylogenetic diversity of Colletotrichum isolates associated with symptomatic tissues of H.brasiliensis from four provinces of China (Hainan, Guangdong, Guangxi, and Yunnan). Based on multi-locus phylogenetic analyses and phenotypic characteristics, five species were distinguished, including two known species (C. fructicola, C. siamense), one novel species of C. gloeosporioides species complex (C. ledongense), and two novel species of C. acutatum species complex (C. bannanense and C. australisinense). Of these, C. siamense and C. australisinense have been recognized as major causative agents of anthracnose of H. brasiliensis.
Preparation from a revisited wet chemical route of phase-pure, monocrystalline and SHG-efficient BiFeO3 nanoparticles for harmonic bio-imaging
Authors: Clarke Gareth , Rogov Andrii , McCarthy Sarah , Bonacina Luigi , Gun’ko Yurii , Galez Christine , Le Dantec Ronan , Volkov Yuri , Mugnier Yannick , Prina-Mello Adriele 
Published: July 2018

AbstractWe present two new synthetic routes for bismuth ferrite harmonic nanoparticles (BiFeO3 HNPs). Both phase-pure and mixed phase BiFeO3 materials were produced after improvement of the solvent evaporation and sol-gel combustion routes. Metal nitrates with a series of dicarboxylic acids (tartronic, tartaric and mucic) were used to promote crystallization. We found that the longer the carbon backbone with a hydroxyl group attached to each carbon, the lower the annealing temperature. We also demonstrate that nanocrystals more readily formed at a given temperature by adding glycerol but to the detriment of phase purity, whereas addition of NaCl in excess with mucic acid promotes the formation of phase-pure, monocrystalline nanoparticles. This effect was possibly associated with a better dispersion of the primary amorphous precursors and formation of intermediate complexes. The nanoparticles have been characterized by XRD, TEM, ζ-potential, photon correlation spectroscopy, two-photon microscopy and Hyper-Rayleigh Scattering measurements. The improved crystallization leads to BiFeO3 HNPs without defect-induced luminescence and with a very high averaged second harmonic efficiency (220 pm/V), almost triple the efficiency previously reported. This development of simple, scalable synthesis routes which yield phase-pure and, crucially, monocrystalline BiFeO3 HNPs demonstrates a significant advance in engineering the properties of nanocrystals for bio-imaging and diagnostics applications.
The influenza virus hemagglutinin head evolves faster than the stalk domain
Authors: Kirkpatrick Ericka , Qiu Xueting , Wilson Patrick C. , Bahl Justin , Krammer Florian 
Published: July 2018

AbstractThe limited ability of current influenza virus vaccines to protect from antigenically drifted or shifted viruses creates a public health problem that has led to the need to develop effective, broadly protective vaccines. While current influenza virus vaccines mostly induce an immune response against the immunodominant and variable head domain of the hemagglutinin, the major surface glycoprotein of the virus, the hemagglutinin stalk domain has been identified to harbor neutralizing B-cell epitopes that are conserved among and even between influenza A virus subtypes. A complete understanding of the differences in evolution between the main target of current vaccines and this more conserved stalk region are missing. Here, we performed an evolutionary analysis of the stalk domains of the hemagglutinin of pre-pandemic seasonal H1N1, pandemic H1N1, seasonal H3N2, and influenza B viruses and show quantitatively for the first time that the stalk domain is evolving at a rate that is significantly slower than that of the head domain. Additionally, we found that the cross-reactive epitopes in the stalk domain targeted by broadly neutralizing monoclonal antibodies are evolving at an even slower rate compared to the full head and stalk regions of the protein. Finally, a fixed-effects likelihood selection analysis was performed for these virus groups in both the head and stalk domains. While several positive selection sites were found in the head domain, only a single site in the stalk domain of pre-pandemic seasonal H1 hemagglutinin was identified at amino acid position 468 (H1 numbering from methionine). This site is not located in or close to the epitopes of cross-reactive anti-stalk monoclonal antibodies. Furthermore, we found that changes in this site do not significantly impact virus binding or neutralization by human anti-stalk antibodies, suggesting that some positive selection in the stalk domain is independent of immune pressures. We conclude that, while the stalk domain does evolve over time, this evolution is slow and, historically, is not directed to aid in evading neutralizing antibody responses.
Engineering biofunctional in vitro vessel models using a multilayer bioprinting technique
Authors: Schöneberg Jan , De Lorenzi Federica , Theek Benjamin , Blaeser Andreas , Rommel Dirk , Kuehne Alexander J. C. , Kießling Fabian , Fischer Horst 
Published: July 2018

AbstractRecent advances in the field of bioprinting have led to the development of perfusable complex structures. However, most of the existing printed vascular channels lack the composition or key structural and physiological features of natural blood vessels or they make use of more easily printable but less biocompatible hydrogels. Here, we use a drop-on-demand bioprinting technique to generate in vitro blood vessel models, consisting of a continuous endothelium imitating the tunica intima, an elastic smooth muscle cell layer mimicking the tunica media, and a surrounding fibrous and collagenous matrix of fibroblasts mimicking the tunica adventitia. These vessel models with a wall thickness of up to 425 µm and a diameter of about 1 mm were dynamically cultivated in fluidic bioreactors for up to three weeks under physiological flow conditions. High cell viability (>83%) after printing and the expression of VE-Cadherin, smooth muscle actin, and collagen IV were observed throughout the cultivation period. It can be concluded that the proposed novel technique is suitable to achieve perfusable vessel models with a biofunctional multilayer wall composition. Such structures hold potential for the creation of more physiologically relevant in vitro disease models suitable especially as platforms for the pre-screening of drugs.
Repeated social defeat stress impairs attentional set shifting irrespective of social avoidance and increases female preference associated with heightened anxiety
Authors: Higashida Shu , Nagai Hirotaka , Nakayama Kazuki , Shinohara Ryota , Taniguchi Masayuki , Nagai Midori , Hikida Takatoshi , Yawata Satoshi , Ago Yukio , Kitaoka Shiho , Narumiya Shuh , Furuyashiki Tomoyuki 
Published: July 2018

AbstractRepeated social defeat stress (R-SDS) induces multiple behavioral changes in mice. However, the relationships between these behavioral changes were not fully understood. In the first experiment, to examine how the social avoidance is related to R-SDS-impaired behavioral flexibility, 10-week-old male C57BL/6N mice received R-SDS followed by the social interaction test and the attentional set shifting task. R-SDS impaired attentional set shifting irrespective of the development of social avoidance. In the second experiment, to examine whether R-SDS affects sexual preference and how this behavioral change is related to the social avoidance and R-SDS-heightened anxiety, another group of 10-week-old male C57BL/6N mice were subjected to R-SDS followed by the social interaction test, the female encounter test and the elevated plus maze test. The anxiety was heightened in the defeated mice without social avoidance, but not in those which showed social avoidance. Furthermore, female preference was increased specifically in the defeated mice which showed heightened anxiety, but was not related to the level of social avoidance. Together, these results showed that attentional set shifting is more sensitive to R-SDS than social interaction, and that female preference is affected by R-SDS in association with heightened anxiety rather than the social avoidance.
Case study: patient-derived clear cell adenocarcinoma xenograft model longitudinally predicts treatment response
Authors: Vargas Roberto , Gopal Priyanka , Kuzmishin Gwendolyn B. , DeBernardo Robert , Koyfman Shlomo A. , Jha Babal K. , Mian Omar Y. , Scott Jacob , Adams Drew J. , Peacock Craig D. , Abazeed Mohamed E. 
Published: July 2018

AbstractThere has been little progress in the use of patient-derived xenografts (PDX) to guide individual therapeutic strategies. In part, this can be attributed to the operational challenges of effecting successful engraftment and testing multiple candidate drugs in a clinically workable timeframe. It also remains unclear whether the ancestral tumor will evolve along similar evolutionary trajectories in its human and rodent hosts in response to similar selective pressures (i.e., drugs). Herein, we combine a metastatic clear cell adenocarcinoma PDX with a timely 3 mouse x 1 drug experimental design, followed by a co-clinical trial to longitudinally guide a patient’s care. Using this approach, we accurately predict response to first- and second-line therapies in so far as tumor response in mice correlated with the patient’s clinical response to first-line therapy (gemcitabine/nivolumab), development of resistance and response to second-line therapy (paclitaxel/neratinib) before these events were observed in the patient. Treatment resistance to first-line therapy in the PDX is coincident with biologically relevant changes in gene and gene set expression, including upregulation of phase I/II drug metabolism (CYP2C18, UGT2A, and ATP2A1) and DNA interstrand cross-link repair (i.e., XPA, FANCE, FANCG, and FANCL) genes. A total of 5.3% of our engrafted PDX collection is established within 2 weeks of implantation, suggesting our experimental designs can be broadened to other cancers. These findings could have significant implications for PDX-based avatars of aggressive human cancers.
Definition, Classification and Diagnosis of Diabetes Mellitus
Authors: Petersmann Astrid , Nauck Matthias , Müller-Wieland Dirk , Kerner Wolfgang , Müller Ulrich , Landgraf Rüdiger , Freckmann Guido , Heinemann Lutz 
Published: July 2018

AbstractAim of recommendations like this one issued by the German Diabetes Association is to provide the GP and diabetologist and his team with information he needs for his daily practice. These recommendations are updated annually. They are written by a group of experts, but they are not evidence based guidelines. This specific recommendation for diabetes diagnosis briefly describes the diabetes types and the different options for diagnosis. Also the caveats and the practical procedure are presented.