bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased Levels of Lipid Oxidation Products in Rheumatically Destructed Bones o f Patients Suffering from Rheumatoid Arthritis
Authors: Jira Wolfgang , Spiteller Gerhard , Richter Andreas 
Published: August 2018

Abstract
The new indicator for lipid peroxidation (LPO) processes -9-hydroxy-10,12-octadecadie-noic acid (9-HODE) -was used to investigate, whether LPO processes are increased in destructed bone material of patients suffering from rheum atoid arthritis (RA) in comparison to surrounded non destructed bone material. The HODE content in destructed bones ex­ceeded that of non destructed ones of the same patient for a factor of about 3. In addition similar increases in leukotoxines and epoxy oleic acid in the destructed bone material were observed, indicating an increase of LPO processes in affected bone parts of pa­tients.
When Do Paederus riparius Rove Beetles (Coleoptera : Staphylinidae) Biosynthesize Their Unique Hemolymph Toxin Pederin?
Authors: Kellner Rupert L. L. 
Published: August 2018

Abstract
Pederin analyses of single Paederus riparius specimens reared from the egg and kept for prolonged periods of time show that only the females are able to biosynthesize the substance. Preimaginal stages efficiently store pederin transferred by the females into their eggs and the males’ pederin content decreases slowly over time. Only males with access to eggs con­ taining the substance moderately increase their pederin load. The females begin to accumu­ late the toxin a few weeks after imaginal eclosion and build up reserves for the egg laying period within 60 days. This is discussed with regard to the life cycle of P. riparius. During reproduction, when the females loose pederin due to maternal transfer, they must replenish their pederin reserves. This biosynthesis is not reflected in the amount found per individual.
Effect of Aging on the Activities of Acetylcholinesterase, Na+, K+-ATPase and Mg2+-ATPase in Rat Pituitary and Hypothalamus
Authors: Tsakiris Stylianos , Angelogianni Panagoula , Stavridis John C. 
Published: August 2018

Abstract
Acetylcholinesterase (AChE), Na+, K+-ATPase and Mg2+-ATPase activities were esti­mated in homogenised rat pituitary and hypothalamus of 4-and 22-month-old rats. AChE activity was not altered in the pituitary of aged com pared to adult rats, while it was found decreased by about 40% in the hypothalamus. Na+,K+-ATPase activity remained stable in the hypothalamus, while it was decreased by about 38% in the pituitary. Mg2+-ATPase activ­ity remained unchanged in the hypothalamus, but was increased by about 83% in the pitu­itary. This pituitary Na+, K+-ATPase inactivation may result in pathological mood and de­creased neural excitability and metabolic energy production in aged animals.The age-related alterations of AChE , Na+, K+-ATPase and Mg2+-ATPase activities may reflect changes in secretion and responses of some hormones of pituitary and hypothalamus.
Interaction of the Anticancer Drug Tamoxifen with the Human Erythrocyte Membrane and Molecular Models
Authors: Suwalsky M. , Hernández P. , Villena F. , Aguilar F. , Sotomayor C. P. 
Published: August 2018

Abstract
Tamoxifen, Anticancer Drug, Erythrocyte Membrane, Phospholipid Bilayer Tamoxifen is a non steroidal antiestrogen drug extensively used in the prevention and treatment of hormone-dependent breast cancer. To evaluate its perturbing effect upon cell membranes it was made to interact with human erythrocytes and molecular models. These consisted of bilayers of dimyristoylphosphatidylcholine (DMPC) and of dimyristoylphospha-tidylethanolamine (DMPE), representative of phospholipids classes located in the outer and inner leaflets of the erythrocyte membrane, respectively. Experiments by fluorescence spectroscopy showed that tamoxifen interacted with DMPC vesicles fluidizing both its polar head and acyl chain regions. These results were confirmed by X-ray diffraction which indi­ cated that tamoxifen perturbed the same regions of the lipid. However, it did not cause any significant structural perturbation to DMPE bilayers. The examination by electron micro­ scopy of human erythrocytes incubated with tamoxifen revealed that they changed their normal discoid shape to stomatocytes. According to the bilayer couple hypothesis, this result means that the drug is inserted in the inner leaflet of the erythrocyte membrane. Given the fact that tamoxifen did not interact with DMPE, it is concluded that it interacted with a protein located in the cytoplasmic moiety of the erythrocyte membrane.
Analysis of the Seed Lipids of Aleurites montana
Authors: Radunz A. , Heb P. , Schmid G. H. 
Published: August 2018

Abstract
The seed lipids of the Chinese tung-oil tree Aleurites montana are composed of 97.2% triglycerides, 2% phospholipids and of 0.8% glycolipids. In the triglycerides 67% of all fatty acids are α-eleostearic acid (configuration C18:3, ∆9 cis, ∆11 trans, ∆13 trans). This acid is bound in the triglycerides in the 1,3 position. Via mercury Il-acetate adducts and subsequent chromatography on silica gel a separation of this fatty acid from the seed fatty acids is possible. By this procedure an a-eleostearic acid-free tung-oil mixture is obtained, which consists by one half of C18:2, 25% of C18:1 and by 25% of the saturated fatty acids C16:0, C18:0 and C14:0.
Visual Recognition Based on Temporal Cortex Cells: Viewer-Centred Processing of Pattern Configuration
Authors: Perrett David I. , Oram Mike W. 
Published: August 2018

Abstract
A model of recognition is described based on cell properties in the ventral cortical stream of visual processing in the primate brain. At a critical intermediate stage in this system, ‘Elaborate’ feature sensitive cells respond selectively to visual features in a way that depends on size (± 1 octave), orientation (± 4 5 °) but does not depend on position within central vision (± 5 °). These features are simple conjunctions of 2-D elements (e.g. a horizontal dark area above a dark smoothly convex area). They can arise either as elements of an object’s surface pattern or as a 3-D component bounded by an object’s external contour. By requiring a combination of several such features without regard to their position within the central region of the visual image, ‘Pattern’ sensitive cells at higher levels can exhibit selectivity for complex configurations that typify objects seen under particular viewing conditions. Given that input features to such Pattern sensitive cells are specified in approximate size and orientation, initial cellular ‘representations’ of the visual appearance of object type (or object example) are also selective for orientation and size. At this level, sensitivity to object view (± 6 0 °) arises because visual features disappear as objects are rotated in perspective. Processing is thus viewer-centred and the neurones only respond to objects seen from particular viewing conditions or ‘object instances’. Combined sensitivity to multiple features (conjunctions of elements) independent of their position, establishes selectivity for the configurations of ob­ject parts (from one view) because rearranged configurations of the same parts yield images lacking some of the 2-D visual features present in the normal configuration.
Different neural populations appear to be selectively tuned to particular components of the same biological object (e.g. face, eyes, hands, legs), perhaps because the independent articulation of these components gives rise to correlated activity in different sets of input visual features. Generalisation over viewing conditions for a given object can be established by hierarchically pooling outputs of view-condition specific cells with pooling operations dependent on the continuity in experience across viewing conditions. Different object parts are seen together and different views are seen in succession when the observer walks around the object. The view specific coding that characterises the selectivity of cells in the temporal lobe can be seen as a natural consequence of selective experience of objects from particular vantage points. View specific coding for the face and body also has great utility in understand­ing complex social signals, a property that may not be feasible with object-centred processing.
Isolation, Characterization and Comparison of Antipeptide and Antiprotein Rabbit Antibodies to the π-Isoform of Glutathione S-Transferase
Authors: Modugno Francesca Di , Rosano Laura , Castelli Mauro , Chersi Alberto 
Published: August 2018

The main linear epitopes of π-glutathione transferase (π-GST, EC 2.5.1.18), an enzyme related to cancer progression in a restricted number of tumours, were identified by testing in ELISA the reactivities of polyclonal anti-π-GST rabbit sera against a panel of 51 overlapping decapeptides, covering the whole 216-residue sequence of the protein. Several major reactivity peaks were detected, each covering two or three adjacent peptides. The most active fragments were then reconstructed by conventional solid-phase synthesis, linked to Sepharose, and used as affinity ligands for isolating specific anti-π-GST antibody subsets.
A second group of antisera was then prepared in rabbits by using as immunogens some of the above described synthetic fragments, linked to a carrier protein, and antipeptide antibodies purified by affinity chromatography. An ELISA test was then performed, using as antigens a panel of peptides and different isoforms of GST, in order to establish whether antibodies isolated from total anti-π-GST sera would display higher reactivity and specificity, as compared to traditional antipeptide antibodies. Binding data clearly confirm that the formers might be indeed better reagents for the detection and possibly quantitation of π-GST.
Synthesis and Anti-Virus Activity of Some Nucleosides Analogues
Authors: Stankova Ivanka G. , Simeonov Mario F. , Maximova Vera , Galabov Angel S. , Golovinsky Evgeny V. 
Published: August 2018

New 3’-, 5’-, 5-bromo-2’-deoxyuridine (3 a-g) and 3’-, 5’-thymidine (4 a-i) analogues with amino acid and peptide residues were synthesized and evaluated for antiviral activity. The influence of long peptide chains, essential amino acids and the effect of this structural modification on the antiviral activity has been also reported.
Three 5-bromo-2’-deoxyuridine derivatives containing glycyl-, glycyl-glycyl-and glycyl-gly-cyl-glycyl-residues (3a, 3b, 3c) showed a strong activity against the herpes virus PsRV and a moderate one vs. HSV-1.
The corresponding thymidine analogues were considerably less effective, and only compounds 4d and 4h showed a borderline effect against PsRV
Acute Effects of Interset Rest Duration on Physiological and Perceptual Responses to Resistance Exercise in Hypoxia
Authors: Lockhart Catriona , Scott Brendan R. , Thoseby Bradley , Dascombe Ben J. 
Published: August 2018

Abstract
Lockhart, C, Scott, BR, Thoseby, B, and Dascombe, BJ. Acute effects of interset rest duration on physiological and perceptual responses to resistance exercise in hypoxia. J Strength Cond Res 34(8): 2241–2249, 2020—This study aimed to determine whether manipulating interset rest periods during resistance training in hypoxia impacts on physiological and perceptual responses to exercise. Twelve healthy males completed 1 repetition maximum (1RM) testing for the bilateral leg extension, before completing 4 separate randomized trials comprising 5 × 10 repetitions of leg extensions at 70% 1RM. Experimental trials were completed in both moderate hypoxia (FIO2 = 15%) and normoxia (FIO2 = 21%), using interset rest periods of both 60 and 180 seconds for each environmental condition. Near-infrared spectroscopy was used to quantify muscle oxygenation of vastus lateralis , and surface electromyography assessed the activation of vastus lateralis and medialis. Blood lactate concentration ([BLa−]) and midthigh circumference were assessed before and immediately after each trial. Heart rate (HR) responses, blood oxygen saturation, and rating of perceived exertion (RPE) were also assessed after each set and the whole session RPE (sRPE). Perceived quadriceps soreness was reported before, immediately after, and at 24 and 48 hours after each trial. Muscle activation (sets 4–5), RPE (sets 3–5), and sRPE were significantly (p < 0.05) higher in the 60-second trials of the resistance exercise protocol. Significant increases (p < 0.01) were observed for [BLa−] and midthigh circumference across sets within each condition. No significant main effect was observed for interset rest duration or environmental condition for muscle oxygenation, HR, or perceived quadriceps soreness. These findings indicate that performing resistance exercise in hypoxia or normoxia with shortened interset rest periods increases muscle activation and perceived exertion, without exacerbating muscle soreness.
Vila União dos Operários, Canoas, RS: luta por moradia e habitabilidade (1980-2014)
Authors: Graebin Cleusa Maria Gomes , Fiorotti Ivo , Graeff Lucas 
Published: August 2018

O estudo tem como objeto as lutas por moradia e melhores condições de habitabilidade ocorridas em Canoas, RS, entre 1980 e 2014. Centra a atenção no processo histórico da ocupação de terreno pertencente a um Jóquei Clube, localizado no Bairro Mathias Velho, dando origem à Vila União dos Operários. Foram 34 anos de luta, por parte dos moradores, que passaram de ocupantes a proprietários de seus lotes. Para dar conta da pesquisa, foram utilizadas fontes escritas e orais, estas produzidas a partir de grupos focais. Teoricamente, o trabalho fundamenta-se em pressupostos da História Social e História Urbana e na Sociologia e Antropologia urbanas, dialogando por meio do conceito de memória social. Constatou-se, a formação de um movimento social de luta que culminou com a posse legal de um território urbano, a partilha dos lotes e a presença de sociabilidade na construção de equipamentos urbanos e de moradias.
Radiation exposure of adrenal vein sampling: a German Multicenter Study
Authors: Fuss C T , Treitl M , Rayes N , Podrabsky P , Fenske W K , Heinrich D A , Reincke M , Petersen T-O , Fassnacht M , Quinkler M , Kickuth R , Hahner S 
Published: August 2018

Objective
Adrenal vein sampling (AVS) represents the current diagnostic standard for subtype differentiation in primary aldosteronism (PA). However, AVS has its drawbacks. It is invasive, expensive, requires an experienced interventional radiologist and comes with radiation exposure. However, exact radiation exposure of patients undergoing AVS has never been examined.

Design and methods
We retrospectively analyzed radiation exposure of 656 AVS performed between 1999 and 2017 at four university hospitals. The primary outcomes were dose area product (DAP) and fluoroscopy time (FT). Consecutively the effective dose (ED) was approximately calculated.

Results
Median DAP was found to be 32.5 Gy*cm2 (0.3–3181) and FT 18 min (0.3–184). The calculated ED was 6.4 mSv (0.1–636). Remarkably, values between participating centers highly varied: Median DAP ranged from 16 to 147 Gy*cm2, FT from 16 to 27 min, and ED from 3.2 to 29 mSv. As main reason for this variation, differences regarding AVS protocols between centers could be identified, such as number of sampling locations, frames per second and the use of digital subtraction angiographies.

Conclusion
This first systematic assessment of radiation exposure in AVS not only shows fairly high values for patients, but also states notable differences among the centers. Thus, we not only recommend taking into account the risk of radiation exposure, when referring patients to undergo AVS, but also to establish improved standard operating procedures to prevent unnecessary radiation exposure.
Extraordinary Genome Instability and Widespread Chromosome Rearrangements During Vegetative Growth
Authors: Möller Mareike , Habig Michael , Freitag Michael , Stukenbrock Eva H 
Published: August 2018

AbstractThe ability to rapidly adapt to changing environments is crucial for the success of pathogens infecting plants and animals. In some eukaryotic pathogens, rapid evolution can be mediated by genome rearrangements, which...The haploid genome of the pathogenic fungus Zymoseptoria tritici is contained on “core” and “accessory” chromosomes. While 13 core chromosomes are found in all strains, as many as eight accessory chromosomes show presence/absence variation and rearrangements among field isolates. The factors influencing these presence/absence polymorphisms are so far unknown. We investigated chromosome stability using experimental evolution, karyotyping, and genome sequencing. We report extremely high and variable rates of accessory chromosome loss during mitotic propagation in vitro and in planta. Spontaneous chromosome loss was observed in 2 to &gt;50% of cells during 4 weeks of incubation. Similar rates of chromosome loss in the closely related Zymoseptoria ardabiliae suggest that this extreme chromosome dynamic is a conserved phenomenon in the genus. Elevating the incubation temperature greatly increases instability of accessory and even core chromosomes, causing severe rearrangements involving telomere fusion and chromosome breakage. Chromosome losses do not affect the fitness of Zymoseptoria tritici in vitro, but some lead to increased virulence, suggesting an adaptive role of this extraordinary chromosome instability.
EGF Receptor–Dependent YAP Activation Is Important for Renal Recovery from AKI
Authors: Chen Jianchun , You Huaizhou , Li Yan , Xu You , He Qian , Harris Raymond C. 
Published: August 2018

Background
Increasing evidence indicates that renal recovery from AKI stems from dedifferentiation and proliferation of surviving tubule epithelial cells. Both EGF receptor (EGFR) and the Hippo signaling pathway are implicated in cell proliferation and differentiation, and previous studies showed that activation of EGFR in renal proximal tubule epithelial cells (RPTCs) plays a critical role in recovery from ischemia-reperfusion injury (IRI). In this study, we explored RPTC activation of Yes-associated protein (YAP) and transcriptional coactivator with PDZ binding motif (TAZ), two key downstream effectors of the Hippo pathway, and their potential involvement in recovery from AKI.

Methods
We used immunofluorescence to examine YAP expression in kidney biopsy samples from patients with clinical AKI and controls (patients with minimal change disease). Studies of RPTC activation of YAP and TAZ used cultured human RPTCs that were exposed to hypoxia-reoxygenation as well as knockout mice (with inducible deletions of Yap, Taz, or both occurring specifically in RPTCs) that were subjected to bilateral IRI.

Results
YAP was activated in RPTCs in kidneys from post-AKI patients and post-IRI mouse kidneys. Inhibition of the interaction of YAP and the TEA domain (TEAD) transcription factor complex by verteporfin or conditional deletion of YAP in RPTCs delayed renal functional and structural recovery from IRI, whereas TAZ deletion had no effect. Activation of the EGFR-PI3K-Akt pathway in response to IRI signaled YAP activation, which promoted cell cycle progression.

Conclusions
This study shows that EGFR-PI3K-Akt–dependent YAP activation plays an essential role in mediating epithelial cell regeneration during kidney recovery from AKI.
Development of a Device for Continuously Monitoring the Parameters of the Winding Structure of Textile Bobbins
Authors: Nuriyev Mahammadali , Ali Veliyev Fazil , İnsaf Hamidov Həmid , Aqagul Sailov Rahib , Mahamad Seydaliyev Ilham , Zargar Jabbarova Ganira 
Published: August 2018

Introduction: The research “Improving the control methods for winding structure at friction drive machines” was conducted at Azerbaijan State Economic University in 2017. Methods: To assess the efficiency of the proposed technique for processing signals, a model of a WA device, which allowsthe unwinding of the package, was designed and manufactured. Results: The authors developed a methodology and a corresponding device to quantitatively assess the parameters of the winding structure by the light-section method. Conclusions: The dimensions adopted while designing the device make it possible to exclude the influence of the non-roundness of the bobbin on the measurement results. Originality: The equipment advancement and quality control improvement are the most urgent issues in textile industry. The quality requirements for package is constantly growing, while this research contribute to maintain it at a corresponding level, which ensures further development of textile industry as a whole. Limitations: The research results can be practically implemented in new winding motion design for spinning frames with cross-winding bobbin.
Características epidemiológicas e clínicas da sepse em um hospital público do Paraná
Authors: Nascimento Santos Zonta Franciele , Gurgel Amaral Velasquez Patricia , Garcia Velasquez Leonardo , Savi Demetrio Luciana , Miranda Danieli , Bortolamedi Diamenes Silva Mirian Carla 
Published: August 2018

Justificativa e Objetivo: A sepse é um problema de saúde pública que gera impacto clínico e econômico. O estudo objetiva descrever as características epidemiológicas e clínicas da sepse em um hospital público do Paraná. Métodos: Trata-se de uma pesquisa de campo exploratória, documental, retrospectiva, com abordagem quantitativa. Os dados foram coletados de prontuários de pacientes admitidos na Unidade de Terapia Intensiva adulto de janeiro de 2012 a janeiro de 2017, para isso utilizou-se um checklist com variáveis clínicas e demográficas. Foram inclusos todos os prontuários de pacientes internados no setor que apresentaram sepse no momento da
internação ou desenvolvida após a admissão. Foi realizada análise de frequência descritiva, distribuição das variáveis independentes e significância pelo teste de Qui-quadrado. Resultados: De um total de 1.557 prontuários, foram incluídos 1112 (71,4%), os quais foram classificados com síndrome da resposta inflamatória sistêmica (13,9%), sepse (39,1 %) ou choque séptico (47,0 %). Houve predominância de pacientes do sexo masculino (55,8%), faixa etária maior de 70 anos (36,3%) e cor branca (92,6%). O tempo de internação foi de até uma semana (50,4%). Prevaleceram pacientes clínicos (42,4%), com infecção nosocomial encontrada em 50,2% dos casos. As principais fontes de infecção foram pulmão (32,9%) e ferida cirúrgica (23,5%). Entre as causas de admissão, complicações respiratórias (19,5%) e politrauma (9,3%) foram as mais
frequentes. As culturas foram positivas em 29,7% dos casos, em sua maioria bacilos gram negativos (12,1%). A mortalidade na SIRS, na sepse e no choque séptico foi de 0,3%, 30,1%, 33,0%, respectivamente. Conclusão: O estudo evidenciou que a sepse acometeu em sua maioria idosos do sexo masculino, e que o foco infecioso foi de origem pulmonar em âmbito nosocomial. Observou-se ainda elevadas taxas de mortalidade, principalmente em casos de choque séptico.