bluuebunny/crossref_metadata_2025_split huggingface dataset:
A quasi-reagentless point-of-care test for nitrite and unaffected by oxygen and cyanide
Authors: Monteiro Tiago , Gomes Sara , Jubete Elena , Añorga Larraitz , Silveira Célia M. , Almeida Maria Gabriela 
Published: February 2019

AbstractThe ubiquitous nitrite is a major analyte in the management of human health and environmental risks. The current analytical methods are complex techniques that do not fulfil the need for simple, robust and low-cost tools for on-site monitoring. Electrochemical reductase-based biosensors are presented as a powerful alternative, due to their good analytical performance and miniaturization potential. However, their real-world application is limited by the need of anoxic working conditions, and the standard oxygen removal strategies are incompatible with point-of-care measurements. Instead, a bienzymatic oxygen scavenger system comprising glucose oxidase and catalase can be used to promote anoxic conditions in aired environments. Herein, carbon screen-printed electrodes were modified with cytochrome c nitrite reductase together with glucose oxidase and catalase, so that nitrite cathodic detection could be performed by cyclic voltammetry under ambient air. The resulting biosensor displayed good linear response to the analyte (2–200 µM, sensitivity of 326 ± 5 mA M−1 cm−2 at −0.8 V; 0.8–150 µM, sensitivity of 511 ± 11 mA M−1 cm−2 at −0.5 V), while being free from oxygen interference and stable up to 1 month. Furthermore, the biosensor’s catalytic response was unaffected by the presence of cyanide, a well-known inhibitor of heme-enzymes.
Different sensitivities of two optical magnetometers realized in the same experimental arrangement
Authors: Put Piotr , Popiołek Kacper , Pustelny Szymon 
Published: February 2019

AbstractIn this article, operation of optical magnetometers detecting static (DC) and oscillating (AC) magnetic fields is studied and comparison of the devices is performed. To facilitate the comparison, the analysis is carried out in the same experimental setup, exploiting nonlinear magneto-optical rotation. In such a system, a control over static-field magnitude or oscillating-field frequency provides detection of strength of the DC or AC fields. Polarization rotation is investigated for various light intensities and AC-field amplitudes, which allows to determine optimum sensitivity to both fields. With the results, we demonstrate that under optimal conditions the AC magnetometer is about ten times more sensitive than its DC counterpart, which originates from different response of the atoms to the fields. Bandwidth of the magnetometers is also analyzed, revealing its different dependence on the light power. Particularly, we demonstrate that bandwidth of the AC magnetometer can be significantly increased without strong deterioration of the magnetometer sensitivity. This behavior, combined with the ability to tune the resonance frequency of the AC magnetometer, provide means for ultra-sensitive measurements of the AC field in a broad but spectrally-limited range, where detrimental role of static-field instability is significantly reduced.
Combination of KIR2DS4 and FcγRIIa polymorphisms predicts the response to cetuximab in KRAS mutant metastatic colorectal cancer
Authors: Borrero-Palacios A. , Cebrián A. , Gómez del Pulgar M. T. , García-Carbonero R. , Garcia-Alfonso P. , Aranda E. , Elez E. , López-López R. , Cervantes A. , Valladares M. , Nadal C. , Viéitez J. M. , Guillén-Ponce C. , Rodríguez J. , Hernández I. , García J. L. , Vega-Bravo R. , Puime-Otin A. , Martínez-Useros J. , Del Puerto-Nevado L. , Rincón R. , Rodríguez-Remírez M. , Rojo F. , García-Foncillas J. 
Published: February 2019

AbstractCetuximab is a standard-of-care treatment forRASwild-type metastatic colorectal cancer (mCRC) but not for those harbor aKRASmutation since MAPK pathway is constitutively activated. Nevertheless, cetuximab also exerts its effect by its immunomodulatory activity despite the presence of RAS mutation. The aim of this study was to determine the impact of polymorphismFcγRIIIaV158F and killer immunoglobulin-like receptor (KIR) genes on the outcome of mCRC patients withKRASmutations treated with cetuximab. This multicenter Phase II clinical trial included 70 mCRC patients withKRASmutated. We foundKIR2DS4gene was significantly associated with OS (HR 2.27; 95% CI, 1.08–4.77; P = 0.03). In non-functional receptor homozygotes the median OS was 2.6 months longer than in carriers of one copy of full receptor. Multivariate analysis confirmedKIR2DS4as a favorable prognostic marker for OS (HR 6.71) in mCRC patients withKRASmutation treated with cetuximab. These data support the potential therapeutic of cetuximab inKRASmutated mCRC carrying non-functional receptorKIR2DS4since these patients significantly prolong their OS even after heavily treatment.KIR2DS4typing could be used as predictive marker for identifying RAS mutated patients that could benefit from combination approaches of anti-EGFR monoclonal antibodies and other immunotherapies to overcome the resistance mediated by mutation in RAS.
Activity-Regulated Cytoskeleton-Associated Protein (Arc/Arg3.1) is Transiently Expressed after Heat Shock Stress and Suppresses Heat Shock Factor 1
Authors: Park A Young , Park Yeon Seung , So Dami , Song In-Kang , Choi Jung-Eun , Kim Hee-Jung , Lee Kong-Joo 
Published: February 2019

AbstractHeat shock proteins are induced by activation of heat shock factor 1 (HSF1) in response to heat shock and protect against heat stress. However, the molecular mechanisms underlying the downstream signal of heat shock have not been fully elucidated. We found that similarly to canonical Hsps, Arc/Arg3.1 is also markedly induced by heat shock and by other cellular stress inducers, including diamide, sodium arsenite and H2O2in various cells. We noted that heat stress–induced Arc/Arg3.1 protein is short lived, with a half-life of <30 min, and is readily degraded by the ubiquitin–proteasome system. Arc/Arg3.1 overexpression inhibited the up-regulation of heat shock–induced Hsp70 and Hsp27, suggesting that Arc/Arg3.1 is a negative regulator of heat shock response (HSR). Studying the effect of Arc/Arg3.1 on HSF1, a major transcription factor in HSR, we found that Arc/Arg3.1 binds to HSF1 and inhibits its binding to the heat shock element in gene promoters, resulting in reduced induction of Hsp27 and Hsp70 mRNAs, without affecting HSF1′s phosphorylation-dependent activation, or nuclear localization. Arc/Arg3.1 overexpression decreased cell survival in response to heat shock. We conclude that Arc/Arg3.1 is transiently expressed after heat shock and negatively regulates HSF1 in the feedback loop of HSR.
Predicting intradialytic hypotension using heart rate variability
Authors: Park Samel , Kim Wook-Joon , Cho Nam-Jun , Choi Chi-Young , Heo Nam Hun , Gil Hyo-Wook , Lee Eun Young 
Published: February 2019

AbstractThis study aimed to identify whether a new method using heart rate variability (HRV) could predict intradialytic hypotension (IDH) for one month in advance for patients undergoing prevalent hemodialysis. A total 71 patients were enrolled, and baseline clinical characteristics and laboratory results were collected when HRV was measured, then, the frequency of IDH was collected during the observation period. HRV parameters included heart rate, R-R interval, the standard deviation of N-N interval, the square root of the mean squared differences of successive NN intervals, very low frequency, low frequency, high frequency, total power, and low frequency/high frequency ratio. During the one-month observation period, 28 patients experienced 85 cases of IDH (10.0% of a total 852 dialysis sessions). Among the clinical and laboratory parameters, ultrafiltration rate, prior history of diabetes, coronary artery disease, or congestive heart failure, age, intact parathyroid hormone level, and history of antihypertensive drug use were integrated into the multivariate model, referred to as a basic model, which showed significant ability to predict IDH (the area-under-curve [AUC], 0.726; p = 0.002). In HRV parameters, changes between the early and middle phases of hemodialysis (referred to Δ) were identified as significant independent variables. New models were built from the combination of Δ values with the basic model. Among them, a model with the highest AUC value (AUC, 804; p < 0.001) was compared to the basic model and demonstrated improved performance when HRV parameters were used (p = 0.049). Based on our results, it is possible that future IDH might be predicted more accurately using HRV.
Assumptions about the positioning of virtual stimuli affect gaze direction estimates during Augmented Reality based interactions
Authors: Binetti Nicola , Cheng Tianchang , Mareschal Isabelle , Brumby Duncan , Julier Simon , Bianchi-Berthouze Nadia 
Published: February 2019

AbstractWe investigated gaze direction determination in dyadic interactions mediated by an Augmented Reality (AR) head-mounted-display. With AR, virtual content is overlaid on top of the real-world scene, offering unique data visualization and interaction opportunities. A drawback of AR however is related to uncertainty regarding the AR user’s focus of attention in social-collaborative settings: an AR user looking in our direction might either be paying attention to us or to augmentations positioned somewhere in between. In two psychophysical experiments, we assessed what impact assumptions concerning the positioning of virtual content attended by an AR user have on other people’s sensitivity to their gaze direction. In the first experiment we found that gaze discrimination was better when the participant was aware that the AR user was focusing on stimuli positioned on their depth plane as opposed to being positioned halfway between the AR user and the participant. In the second experiment, we found that this modulatory effect was explained by participants’ assumptions concerning which plane the AR user was focusing on, irrespective of these being correct. We discuss the significance of AR reduced gaze determination in social-collaborative settings as well as theoretical implications regarding the impact of this technology on social behaviour.
Reduced hepatocellular lipid accumulation and energy metabolism in patients with long standing type 1 diabetes mellitus
Authors: Wolf Peter , Fellinger Paul , Pfleger Lorenz , Smajis Sabina , Beiglböck Hannes , Gajdošík Martin , Anderwald Christian-Heinz , Trattnig Siegfried , Luger Anton , Winhofer Yvonne , Krššák Martin , Krebs Michael 
Published: February 2019

AbstractThe prevalence of obesity and metabolic syndrome increases in patients with type 1 diabetes mellitus (T1DM). In the general population this is linked with ectopic lipid accumulation in liver (HCL) and skeletal muscle (IMCL), representing hallmarks in the development of insulin resistance. Moreover, hepatic mitochondrial activity is lower in newly diagnosed patients with T1DM. If this precedes later development of diabetes related fatty liver disease is currently not known. This study aims to investigate energy metabolism in liver (kATP) and skeletal muscle (kCK) and its impact on HCL, IMCL, cardiac fat depots and heart function in 10 patients with long standing T1DM compared to 11 well-matched controls by 31P/1H magnetic resonance spectroscopy. HCL was almost 70% lower in T1DM compared to controls (6.9 ± 5% vs 2.1 ± 1.3%; p = 0.030). Also kATP was significantly reduced (0.33 ± 0.1 s−1vs 0.17 ± 0.1 s−1; p = 0.018). In T1DM, dose of basal insulin strongly correlated with BMI (r = 0.676, p = 0.032) and HCL (r = 0.643, p = 0.045), but not with kATP. In the whole cohort, HCL was significantly associated with BMI (r = 0.615, p = 0.005). In skeletal muscle kCK was lower in patients with T1DM (0.25 ± 0.05 s−1vs 0.31 ± 0–04 s−1; p = 0.039). No significant differences were found in IMCL. Cardiac fat depots as well as heart function were not different. Our results in patients with long standing T1DM show that HCL is lower compared to matched controls, despite reduced energy metabolism in liver and skeletal muscle.
Remarkable N2O emissions by draining fallow paddy soil and close link to the ammonium-oxidizing archaea communities
Authors: Wang Ling , Li Kun , Sheng Rong , Li Zhaohua , Wei Wenxue 
Published: February 2019

AbstractFallow paddies experience natural flooding and draining water status due to rainfall and evaporation, which could induce considerable nitrous oxide (N2O) emissions and need to be studied specially. In this study, intact soil columns were collected from a fallow paddy field and the flooding-draining process was simulated in a microcosm experiment. The results showed that both N2O concentrations in the soil and N2O emission rates were negligible during flooding period, which were greatly elevated by draining the fallow paddy soil. The remarkable N2O concentrations in the soil and N2O emission/h during draining both had significant relationships with the Arch-amoA gene (P < 0.01) but not the Bac-amoA, narG, nirK, nirS, and nosZ genes, indicating that the ammonium-oxidizing archaea (AOA) might be the important players in soil N2O net production and emissions after draining. Moreover, we observed that N2O concentrations in the upper soil layers (0–10 cm) were not significantly different from that in the 10–20 cm layer under draining condition (P > 0.05). However, the number of AOA and the nitrification substrate (NH4+-N) in the 0–10 cm layer were significantly higher than in the 10–20 cm layer (P < 0.01), indicating N2O production in the 0–10 cm layer might be higher than the measured concentration and would contribute considerably to N2O emissions as shorter distance of gas diffusion to the soil surface.
The role of nuclear factor erythroid-2-related factor 2 expression in radiocontrast-induced nephropathy
Authors: Kim Ji Eun , Bae So Yeon , Ahn Shin Young , Kwon Young Joo , Ko Gang Jee 
Published: February 2019

AbstractRadiocontrast-induced nephropathy (CIN) is the third most common cause of acute renal failure. The pathophysiology of CIN is related to tubular injury caused by oxidative stress, and nuclear factor erythroid-2-related factor 2 (Nrf2) is critical in coordinating intracellular antioxidative processes. We thus investigated the role of Nrf2 in CIN. CIN was established in mice and in NRK-52E cells via iohexol administration according to the protocols of previous studies. To determine the role of Nrf2 in CIN, Nrf2 expression was reduced in vivo using Nrf2 knockout (KO) mice (B6.129 × 1-Nfe2 l2tm1Ywk/J) and in vitro with siRNA treatment targeting Nrf2. Increased Nrf2 expression was observed after iohexol treatment both in vivo and in vitro. Serum creatinine at 24 h after iohexol injection was significantly higher in KO mice than in wild-type (WT) mice. Histologic examination showed that iohexol-induced tubular vacuolization and structural disruption were aggravated in Nrf2 KO mice. Significant increases in apoptosis and F4/80(+) inflammatory cell infiltration were demonstrated in KO mice compared to WT mice. In addition, the increase in reactive oxygen species after iohexol treatment was augmented by Nrf2 inhibition both in vivo and in vitro. Nrf2 may be implicated in the pathogenesis of CIN via the modulation of antioxidant, anti-apoptotic, and anti-inflammatory processes.
Coupling of a viral K+-channel with a glutamate-binding-domain highlights the modular design of ionotropic glutamate-receptors
Authors: Schönrock Michael , Thiel Gerhard , Laube Bodo 
Published: February 2019

AbstractIonotropic glutamate receptors (iGluRs) mediate excitatory neuronal signaling in the mammalian CNS. These receptors are critically involved in diverse physiological processes; including learning and memory formation, as well as neuronal damage associated with neurological diseases. Based on partial sequence and structural similarities, these complex cation-permeable iGluRs are thought to descend from simple bacterial proteins emerging from a fusion of a substrate binding protein (SBP) and an inverted potassium (K+)-channel. Here, we fuse the pore module of the viral K+-channel KcvATCV-1 to the isolated glutamate-binding domain of the mammalian iGluR subunit GluA1 which is structural homolog to SBPs. The resulting chimera (GluATCV*) is functional and displays the ligand recognition characteristics of GluA1 and the K+-selectivity of KcvATCV-1. These results are consistent with a conserved activation mechanism between a glutamate-binding domain and the pore-module of a K+-channel and support the expected phylogenetic link between the two protein families.
Maternal, placental and cordonal metallomic profiles in gestational diabetes mellitus
Authors: Roverso Marco , Di Marco Valerio , Badocco Denis , Pastore Paolo , Calanducci Marilia , Cosmi Erich , Visentin Silvia 
Published: February 2019

Abstract
76 pregnant women, among them 38 affected by gestational diabetes mellitus (GDM) and 38 control subjects, were recruited at the University Hospital of Padua (Italy). Placenta samples, maternal whole blood and umbilical cord whole blood were collected after delivery and analysed via ICP-MS to determine the metallome, i.e. the whole elemental content. Results were statistically evaluated to evidence the correlation between the elemental concentrations in all samples and the presence of the disease. The results obtained in whole cord blood showed that many elements were correlated with GDM: Ca, Cu, Na, and Zn were present in higher concentration in GDM cord blood than in control samples, whereas Fe, K, Mn, P, Rb, S and Si showed an opposite trend. It was also highlighted that the cord blood from GDM patients exhibited an elemental composition more similar to that of the mother blood compared with the cord blood from control subjects. These results, in part interpreted in the light of the literature, open the possibility to use cord blood as a GDM marker, thus helping to delineate more accurate nutritional guidelines for pregnant women and to explain the biochemical processes occurring in the fetus and placenta during GDM.
The Recline and Head Lift Exercises: A Randomized Clinical Trial Comparing Biomechanical Swallowing Outcomes and Perceived Effort in Healthy Older Adults
Authors: Fujiki Robert Brinton , Oliver Abby J. , Malandraki Jaime Bauer , Wetzel Dawn , Craig Bruce A. , Malandraki Georgia A. 
Published: February 2019

Purpose
The aim of this study was to compare biomechanical swallowing outcomes and perceived effort as well as detraining effects of the established Head Lift Exercise (HLE) and the novel Recline Exercise (RE) in healthy older adults.

Method
Twenty-two healthy older adults were randomized to perform either the RE or the HLE for a period of 6 weeks. Subjects underwent videofluoroscopic swallowing studies at 3 time points (baseline, postexercise, and following a 6-week detraining period). Primary outcome measures included biomechanical measures of superior and anterior hyoid excursion and upper esophageal sphincter opening, obtained using kinematic analyses on the recorded swallows. Perceived exertion ratings during exercise, as measured by the Borg scale, were included as a secondary outcome measure. Linear mixed-effects models were utilized to compare exercise groups and evaluation time points.

Results

The 2 exercise groups did not differ significantly in age, body mass index, or body fat percentage at baseline. Significant postexercise increases were seen in superior hyoid excursion,
F
(2, 36.7) = 24.01,
p
≤ .0001, and anterior hyoid excursion,
F
(2, 36.7) = 5.40,
p
= .0088, for both exercise groups. Upper esophageal sphincter opening did not increase significantly following the exercise regimens,
F
(2, 36.5) = 2.14,
p
= .1322. Both groups displayed a significant decrease in perceived exertion levels over the course of the exercises,
F
(5, 98) = 23.73,
p
≤ .0001. On average, Borg ratings were 20% lower for the RE group than the HLE group at all time points,
F
(5, 20) = 7.94,
p
= .0106, indicating that this exercise was perceived as easier to perform. Eighteen participants were followed after detraining, and no differences in detraining effects were seen between groups. In general, gains in biomechanical measures were better maintained on larger bolus types.

Conclusions
In healthy older adults, the HLE and the RE produced similar gains and detraining effects in biomechanical swallow outcomes. The RE exercise, however, required significantly less effort. These findings suggest that the RE is easier to perform for healthy older adults and thus may be a valuable treatment option for individuals who have difficulty performing the HLE. Further investigation in patients with dysphagia is warranted.

Supplemental Material

https://doi.org/10.23641/asha.7742897
Measure and analysis of 4H-SiC Schottky barrier height with Mo contacts
Authors: Zhang Teng , Raynaud Christophe , Planson Dominique 
Published: February 2019

Current–voltage (I–V) and capacitance–voltage (C–V) characteristics of Schottky Mo/4H-SiC diodes have been measured and analyzed as a function of temperature between 80 and 400 K. The I–V characteristics significantly deviate from ideal characteristics predicted by the thermionic emission model because of the inhomogeneity of Schottky contact. After a brief review of the different existing models, the main parameters (ideality factor, barrier height, and effective Richardson constant) of both diodes have been extracted in the frame of a Gaussian barrier height distribution model, whose mean and standard deviation are linearly dependent on voltage and temperature, as well as in the context of the potential fluctuation model. The results are compared with the values extracted by C–V and the values in the literature. A link is established between these two models. Diodes of different I–V characteristics, either identified as single barrier or double barrier, have been analyzed by Deep Level Transient Spectroscopy (DLTS) to investigate the deep level defects present. No noticeable difference has been found.
Management of mTOR inhibitors oral mucositis: current state of knowledge
Authors: Calvo Anne Sophie , Rochefort Juliette , Javelot Marie José , Descroix Vianney , Lescaille Géraldine 
Published: February 2019

Introduction:Mucositis is a well-known side effect of classic anticancer treatments (chemotherapy and radiotherapy). Thanks to the major developments in personalizing treatments through the development of targeted treatment, various specific intraoral lesions have been described. Purpose:mTOR inhibitors are targeted anticancer treatments that are used to treat various cancer types. They can cause intraoral ulcerations that can be serious, and that can lead to a dose reduction or the anticancer treatment being stopped altogether. The management of these disabling and painful lesions is a major part of ensuring the efficiency of the cancer treatments. The objective of this article is to evaluate the current knowledge about the different treatments used nowadays, especially the preventive treatments. Conclusion:An efficient management of the lesions is a major part of the management of patients treated with mTOR inhibitors and should be carried out by the oral cavity specialists.