bluuebunny/crossref_metadata_2025_split huggingface dataset:
Common variants of ARID1A and KAT2B are associated with obesity in Indian adolescents
Authors: Giri Anil K. , Parekatt Vaisak , Dwivedi Om Prakash , Banerjee Priyanka , Bandesh Khushdeep , Prasad Gauri , Tandon Nikhil , Bharadwaj Dwaipayan 
Published: February 2018

AbstractObesity involves alterations in transcriptional programs that can change in response to genetic and environmental signals through chromatin modifications. Since chromatin modifications involve different biochemical, neurological and molecular signaling pathways related to energy homeostasis, we hypothesize that genetic variations in chromatin modifier genes can predispose to obesity. Here, we assessed the associations between 179 variants in 35 chromatin modifier genes and overweight/obesity in 1283 adolescents (830 normal weight and 453 overweight/obese). This was followed up by the replication analysis of associated signals (18 variants in 8 genes) in 2247 adolescents (1709 normal weight and 538 overweight/obese). Our study revealed significant associations of two variants rs6598860 (OR = 1.27, P = 1.58 × 10–4) and rs4589135 (OR = 1.22, P = 3.72 × 10–4) in ARID1A with overweight/obesity. We also identified association of rs3804562 (β = 0.11, P = 1.35 × 10–4) in KAT2B gene with BMI. In conclusion, our study suggests a potential role of ARID1A and KAT2B genes in the development of obesity in adolescents and provides leads for further investigations.
miR-27b regulates myogenic proliferation and differentiation by targeting Pax3 in goat
Authors: Ling Ying-Hui , Sui Meng-Hua , Zheng Qi , Wang Kang-Yan , Wu Hao , Li Wen-Yong , Liu Yong , Chu Ming-Xing , Fang Fu-Gui , Xu Li-Na 
Published: February 2018

AbstractThis study found that miR-27 is expressed in muscle and regulates muscle proliferation and differentiation. We explored the function and regulatory mechanism of miR-27b in goat muscle proliferation and differentiation. Compared with the Boer goat, higher expression of miR-27b was observed in all of the collected muscle tissues of Anhuai goat, excluding the kidney, whereas the opposite expression pattern was observed for Pax3, which showed lower expression in Anhuai goat. Expression of miR-27b decreased gradually during the proliferation of skeletal muscle satellite cells in Anhuai goat and increased during differentiation; however, the expression pattern of Pax3 was opposite. The regulatory activity of miR-27b demonstrated that miR-27b inhibited the proliferation of skeletal muscle satellite cells, but promoted their differentiation. Moreover, function research demonstrated that Pax3 negatively regulated myogenic differentiation of goat skeletal muscle satellite cells, but accelerated their proliferation. The results of a dual-luciferase reporter analysis showed that miR-27b directly targeted the 3’-untranslated regions of Pax3 mRNA, and western blot and immunofluorescence staining analyses showed that miR-27b inhibited expression of the Pax3 protein. In goats, miR-27b can regulate myogenic proliferation and differentiation by targeting Pax3.
Graphene Electrode Enabling Electrochromic Approaches for Daylight-Dimming Applications
Authors: Kim Joo Yeon , Cho Nam Sung , Cho Seungmin , Kim Kisoo , Cheon Sanghoon , Kim Kyuwon , Kang Seung-Youl , Cho Seong M. , Lee Jeong-Ik , Oh Ji-Young , Kim Yong-Hae , Ryu Hojun , Hwang Chi-Sun , Kim Sujung , Ah Chil Seong , Kim Tae-Youb 
Published: February 2018

AbstractFor environmental reason, buildings increasingly install smart windows, which can dim incoming daylight based on active electrochromic devices (ECDs). In this work, multi-layered graphene (MLG) was investigated as an ECD window electrode, to minimize carbon dioxide (CO2) emissions by decreasing the electricity consumption for building space cooling and heating and as an alternative to the transparent conductor tin-doped indium oxide (ITO) in order to decrease dependence on it. Various MLG electrodes with different numbers of graphene layers were prepared with environmentally friendly poly(3,4-ethylenedioxythiophene):poly(styrene-sulfonate) (PEDOT:PSS) to produce ECD cells. Tests demonstrated the reproducibility and uniformity in optical performance, as well as the flexibility of the ECD fabrication. With the optimized MLG electrode, the ECD cells exhibited a very fast switching response for optical changes from transparent to dark states of a few hundred msec.
Sophora flavescens protects against mycobacterial Trehalose Dimycolate-induced lung granuloma by inhibiting inflammation and infiltration of macrophages
Authors: Liu Dehua , Chan Ben Chung-Lap , Cheng Ling , Tsang Miranda Sin-Man , Zhu Jing , Wong Chun-Wai , Jiao Delong , Chan Helen Yau-Tsz , Leung Ping Chung , Lam Christopher Wai-Kei , Wong Chun Kwok 
Published: February 2018

AbstractThe immune system responds to Mycobacterium tuberculosis (MTB) infection by forming granulomas to quarantine the bacteria from spreading. Granuloma-mediated inflammation is a cause of lung destruction and disease transmission. Sophora flavescens (SF) has been demonstrated to exhibit bactericidal activities against MTB. However, its immune modulatory activities on MTB-mediated granulomatous inflammation have not been reported. In the present study, we found that flavonoids from Sophora flavescens (FSF) significantly suppressed the pro-inflammatory mediators released from mouse lung alveolar macrophages (MH-S) upon stimulation by trehalose dimycolate (TDM), the most abundant lipoglycan on MTB surface. Moreover, FSF reduced adhesion molecule (LFA-1) expression on MH-S cells after TDM stimulation. Furthermore, FSF treatment on TDM-activated lung epithelial (MLE-12) cells significantly downregulated macrophage chemoattractant protein (MCP-1/CCL2) expression, which in turn reduced the in vitro migration of MH-S to MLE-12 cells. In addition, FSF increased the clearance of mycobacterium bacteria (Mycobacterium aurum) in macrophages. FSF mainly affected the Mincle-Syk-Erk signaling pathway in TDM-activated MH-S cells. In TDM-induced mouse granulomas model, oral administration with FSF significantly suppressed lung granulomas formation and inflammation. These findings collectively implicated an anti-inflammatory role of FSF on MTB-mediated granulomatous inflammation, thereby providing evidence of FSF as an efficacious adjunct treatment during mycobacterial infection.
Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer
Authors: Mansour W. Y. , Tennstedt P. , Volquardsen J. , Oing C. , Kluth M. , Hube-Magg C. , Borgmann K. , Simon R. , Petersen C. , Dikomey E. , Rothkamm K. 
Published: February 2018

AbstractHere we report that PTEN contributes to DNA double-strand break (DSB) repair via homologous recombination (HR), as evidenced by (i) inhibition of HR in a reporter plasmid assay, (ii) enhanced sensitivity to mitomycin-C or olaparib and (iii) reduced RAD51 loading at IR-induced DSBs upon PTEN knockdown. No association was observed between PTEN-status and RAD51 expression either in-vitro or in-vivo in a tissue microarray of 1500 PTEN-deficient prostate cancer (PC) samples. PTEN depletion and sustained activation of AKT sequestered CHK1 in the cytoplasm, thus impairing the G2/M-checkpoint after irradiation. Consistently, AKT inhibition recovered the G2/M-checkpoint and restored HR efficiency in PTEN-depleted cells. We show that, although PTEN loss correlates with a worse prognosis, it may predict for improved response of PC patients to radiotherapy. Further, we provide evidence for the use of PTEN as a biomarker for predicting the response to PARP inhibitors as radiosensitizing agents in prostate cancer. Collectively, these data implicate PTEN in maintaining genomic stability by delaying G2/M-phase progression of damaged cells, thus allowing time for DSB repair by HR. Furthermore, we identify PTEN-status in PC as a putative predictor of (i) radiotherapy response and (ii) response to treatment with PARP inhibitor alone or combined with radiotherapy.
Identification of genomic differences among peripheral arterial beds in atherosclerotic and healthy arteries
Authors: Steenman Marja , Espitia Olivier , Maurel Blandine , Guyomarch Beatrice , Heymann Marie-Françoise , Pistorius Marc-Antoine , Ory Benjamin , Heymann Dominique , Houlgatte Rémi , Gouëffic Yann , Quillard Thibaut 
Published: February 2018

AbstractCalcification is independently associated with cardiovascular events and morbidity. The calcification burden in atherosclerotic lesions quantitatively and qualitatively differs between arterial beds. Cardiovascular risk factors (CVRF) differentially affect plaque development between arterial beds. The aim of this study was to evaluate the impact of CVRF on atherosclerotic plaque calcification and to further study the molecular arterial heterogeneity that could account for these differences. Histological analysis was performed on atherosclerotic plaques from 153 carotid, 97 femoral and 28 infrapopliteal arteries. CVRF showed minor associations with plaque calcification: age and hypertension affected only the overall presence of calcification but not the type of the calcification, which significantly differed between arterial beds. Transcriptome analysis revealed distinct gene expression profiles associated with each territory in atherosclerotic and healthy arteries. Canonical pathway analysis showed the preferential involvement of immune system-related processes in both atherosclerotic and healthy carotid arteries. Bone development-related genes were among those mostly enriched in atherosclerotic and healthy femoral arteries, which are more prone to developing endochondral calcification. This study highlights the heterogeneous nature of arteries from different peripheral vascular beds and contributes to a better understanding of atherosclerosis formation and evolution.
Multi-modal survey of Adélie penguin mega-colonies reveals the Danger Islands as a seabird hotspot
Authors: Borowicz Alex , McDowall Philip , Youngflesh Casey , Sayre-McCord Thomas , Clucas Gemma , Herman Rachael , Forrest Steven , Rider Melissa , Schwaller Mathew , Hart Tom , Jenouvrier Stéphanie , Polito Michael J. , Singh Hanumant , Lynch Heather J. 
Published: February 2018

AbstractDespite concerted international effort to track and interpret shifts in the abundance and distribution of Adélie penguins, large populations continue to be identified. Here we report on a major hotspot of Adélie penguin abundance identified in the Danger Islands off the northern tip of the Antarctic Peninsula (AP). We present the first complete census of Pygoscelis spp. penguins in the Danger Islands, estimated from a multi-modal survey consisting of direct ground counts and computer-automated counts of unmanned aerial vehicle (UAV) imagery. Our survey reveals that the Danger Islands host 751,527 pairs of Adélie penguins, more than the rest of AP region combined, and include the third and fourth largest Adélie penguin colonies in the world. Our results validate the use of Landsat medium-resolution satellite imagery for the detection of new or unknown penguin colonies and highlight the utility of combining satellite imagery with ground and UAV surveys. The Danger Islands appear to have avoided recent declines documented on the Western AP and, because they are large and likely to remain an important hotspot for avian abundance under projected climate change, deserve special consideration in the negotiation and design of Marine Protected Areas in the region.
Metabolic Reprogramming in Amyotrophic Lateral Sclerosis
Authors: Szelechowski M. , Amoedo N. , Obre E. , Léger C. , Allard L. , Bonneu M. , Claverol S. , Lacombe D. , Oliet S. , Chevallier S. , Le Masson G. , Rossignol R. 
Published: February 2018

AbstractMitochondrial dysfunction in the spinal cord is a hallmark of amyotrophic lateral sclerosis (ALS), but the neurometabolic alterations during early stages of the disease remain unknown. Here, we investigated the bioenergetic and proteomic changes in ALS mouse motor neurons and patients’ skin fibroblasts. We first observed that SODG93A mice presymptomatic motor neurons display alterations in the coupling efficiency of oxidative phosphorylation, along with fragmentation of the mitochondrial network. The proteome of presymptomatic ALS mice motor neurons also revealed a peculiar metabolic signature with upregulation of most energy-transducing enzymes, including the fatty acid oxidation (FAO) and the ketogenic components HADHA and ACAT2, respectively. Accordingly, FAO inhibition altered cell viability specifically in ALS mice motor neurons, while uncoupling protein 2 (UCP2) inhibition recovered cellular ATP levels and mitochondrial network morphology. These findings suggest a novel hypothesis of ALS bioenergetics linking FAO and UCP2. Lastly, we provide a unique set of data comparing the molecular alterations found in human ALS patients’ skin fibroblasts and SODG93A mouse motor neurons, revealing conserved changes in protein translation, folding and assembly, tRNA aminoacylation and cell adhesion processes.
Increased Spatial Variability and Intensification of Extreme Monsoon Rainfall due to Urbanization
Authors: Paul Supantha , Ghosh Subimal , Mathew Micky , Devanand Anjana , Karmakar Subhankar , Niyogi Dev 
Published: February 2018

AbstractWhile satellite data provides a strong robust signature of urban feedback on extreme precipitation; urbanization signal is often not so prominent with station level data. To investigate this, we select the case study of Mumbai, India and perform a high resolution (1 km) numerical study with Weather Research and Forecasting (WRF) model for eight extreme rainfall days during 2014–2015. The WRF model is coupled with two different urban schemes, the Single Layer Urban Canopy Model (WRF-SUCM), Multi-Layer Urban Canopy Model (WRF-MUCM). The differences between the WRF-MUCM and WRF-SUCM indicate the importance of the structure and characteristics of urban canopy on modifications in precipitation. The WRF-MUCM simulations resemble the observed distributed rainfall. WRF-MUCM also produces intensified rainfall as compared to the WRF-SUCM and WRF-NoUCM (without UCM). The intensification in rainfall is however prominent at few pockets of urban regions, that is seen in increased spatial variability. We find that the correlation of precipitation across stations within the city falls below statistical significance at a distance greater than 10 km. Urban signature on extreme precipitation will be reflected on station rainfall only when the stations are located inside the urban pockets having intensified precipitation, which needs to be considered in future analysis.
Global phenotypic characterisation of human platelet lysate expanded MSCs by high-throughput flow cytometry
Authors: Reis Monica , McDonald David , Nicholson Lindsay , Godthardt Kathrin , Knobel Sebastian , Dickinson Anne M. , Filby Andrew , Wang Xiao-nong 
Published: February 2018

AbstractMesenchymal stromal cells (MSCs) are a promising cell source to develop cell therapy for many diseases. Human platelet lysate (PLT) is increasingly used as an alternative to foetal calf serum (FCS) for clinical-scale MSC production. To date, the global surface protein expression of PLT-expended MSCs (MSC-PLT) is not known. To investigate this, paired MSC-PLT and MSC-FCS were analysed in parallel using high-throughput flow cytometry for the expression of 356 cell surface proteins. MSC-PLT showed differential surface protein expression compared to their MSC-FCS counterpart. Higher percentage of positive cells was observed in MSC-PLT for 48 surface proteins, of which 13 were significantly enriched on MSC-PLT. This finding was validated using multiparameter flow cytometry and further confirmed by quantitative staining intensity analysis. The enriched surface proteins are relevant to increased proliferation and migration capacity, as well as enhanced chondrogenic and osteogenic differentiation properties. In silico network analysis revealed that these enriched surface proteins are involved in three distinct networks that are associated with inflammatory responses, carbohydrate metabolism and cellular motility. This is the first study reporting differential cell surface protein expression between MSC-PLT and MSC-FSC. Further studies are required to uncover the impact of those enriched proteins on biological functions of MSC-PLT.
Comparison of the diagnostic accuracy of cone beam computed tomography and radiography for scaphoid fractures
Authors: Neubauer Jakob , Benndorf Matthias , Ehritt-Braun Claudia , Reising Kilian , Yilmaz Tayfun , Klein Christopher , Zajonc Horst , Kotter Elmar , Langer Mathias , Goerke Sebastian Moritz 
Published: February 2018

AbstractThe aim of this study was to evaluate and compare the diagnostic accuracy, the inter-rater agreement and raters’ certainty of cone beam computed tomography (CBCT) and radiography for the detection of scaphoid fractures. Our hypothesis is that the CBCT has a higher diagnostic accuracy for scaphoid fractures than radiography. We retrospectively analysed patients who underwent both radiography and CBCT examinations within 4 days to rule out a scaphoid fracture over a 2-year period in our institution. 4 blinded radiologists and orthopaedic surgeons independently rated the images regarding the presence of a scaphoid fracture. The reference standard was evaluated by two radiologists in a consensus reading. Inter-rater correlation was evaluated, pooled sensitivity, specificity, positive and negative predictive values were calculated and compared. 102 patients met the inclusion criteria. 52% of them had a scaphoid fracture. The inter-rater correlation was higher in the CBCT compared to radiography (P < 0.001). Sensitivity, specificity, positive and negative predictive values were higher for CBCT than for radiography (P < 0.019). Observers’ fracture classifications showed a higher correlation with the reference standard in the CBCT. Observers’ certainty for fracture detection and classification were higher in the CBCT. CBCT shows a higher diagnostic accuracy for scaphoid fractures than radiography.
Retinal microvascular parameters are not associated with reduced renal function in a study of individuals with type 2 diabetes
Authors: McKay Gareth J. , Paterson Euan N. , Maxwell Alexander P. , Cardwell Christopher C. , Wang Ruixuan , Hogg Stephen , MacGillivray Thomas J. , Trucco Emanuele , Doney Alexander S. 
Published: February 2018

AbstractThe eye provides an opportunistic “window” to view the microcirculation. There is published evidence of an association between retinal microvascular calibre and renal function measured by estimated glomerular filtration rate (eGFR) in individuals with diabetes mellitus. Beyond vascular calibre, few studies have considered other microvascular geometrical features. Here we report novel null findings for measures of vascular spread (vessel fractal dimension), tortuosity, and branching patterns and their relationship with renal function in type 2 diabetes over a mean of 3 years. We performed a nested case-control comparison of multiple retinal vascular parameters between individuals with type 2 diabetes and stable (non-progressors) versus declining (progressors) eGFR across two time points within a subset of 1072 participants from the GoDARTS study cohort. Retinal microvascular were measured using VAMPIRE 3.1 software. In unadjusted analyses and following adjustment for age, gender, systolic blood pressure, HbA1C, and diabetic retinopathy, no associations between baseline retinal vascular parameters and risk of eGFR progression were observed. Cross-sectional analysis of follow-up data showed a significant association between retinal arteriolar diameter and eGFR, but this was not maintained following adjustment. These findings are consistent with a lack of predictive capacity for progressive loss of renal function in type 2 diabetes.
Single Ag nanoparticle collisions within a dual-electrode micro-gap cell
Authors: McKelvey Kim , Robinson Donald A. , Vitti Nicholas J. , Edwards Martin A. , White Henry S. 
Published: February 2018

An adjustable width (between 600 nm and 20 μm) gap between two Au microelectrodes is used to probe the electrodissolution dynamics of single Ag nanoparticles.
Predictive Factors for Vestibular Loss in Children With Hearing Loss
Authors: Janky Kristen L. , Thomas Megan L. A. , High Robin R. , Schmid Kendra K. , Ogun Oluwaseye Ayoola 
Published: February 2018

Purpose
The aim of this study was to determine if there are factors that can predict whether a child with hearing loss will also have vestibular loss.

Method
A retrospective chart review was completed on 186 children with hearing loss seen at Boys Town National Research Hospital for vestibular testing from 1999 to 2015 through neurosensory genetics clinic or cochlear implant candidacy. Each child's medical chart was reviewed to obtain the following data: vestibular loss severity (classified as normal, bilateral, or mild to moderate), degree of hearing loss (bilateral pure-tone average [PTA]), imaging abnormalities (classified as “normal” or “abnormal”), parental concerns for gross motor delay (classified as “yes, there is concern” or “no, there is not a concern”), parent report of age when their child sat (months) and walked independently (months), comorbidities (classified as “yes” if there were 1 or more comorbidities or “no” if there were no comorbidities), and score on the Developmental Profile-3.

Results
Children were grouped according to vestibular loss severity; 115 children had normal vestibular function, 31 had bilateral vestibular loss, and 40 had mild-to-moderate vestibular loss. As severity of vestibular loss increased, children (a) sat and walked later, (b) scored more poorly on the Developmental Profile-3 physical subscale, (c) had more severe hearing loss, (d) had parents who more frequently reported concern for gross motor delay, and (e) were more likely to have other comorbidities. Of these factors, age-to-sit, age-to-walk, PTA, and parental concerns for gross motor developmental delay had the greatest ability to differentiate children with vestibular loss from children with normal vestibular function.
For age-to-sit, using a cutoff value of 7.25 months yielded a sensitivity of 62% and a specificity of 81%. For age-to-walk, a cutoff value of 14.5 months yielded a sensitivity of 78% and a specificity of 77%. For PTA for the neurosensory genetics group, a cutoff value of 40 dB yielded a sensitivity of 80% and a specificity of 55%; however, a cutoff value of 66 dB yielded a sensitivity of 33% and an improved specificity of 91%.

Conclusions
A referral for vestibular evaluation should be considered for children whose hearing loss is greater than 66 dB and particularly those who sit later than 7.25 months or walk later than 14.5 months or whose parents report concerns for gross motor development. Collectively, these factors appear to be more sensitive for identifying children with bilateral vestibular loss compared with children with mild-to-moderate vestibular loss. Because of the benefit of physical therapy, children identified with vestibular loss should then be referred to physical therapy for further evaluation and treatment.