bluuebunny/crossref_metadata_2025_split huggingface dataset:
Constipation-Associated Stercoral Colitis
Authors: Proulx Erik , Glass Casey 
Published: August 2018

Abstract
A 9-year-old white girl with a history of constipation presented to the emergency department with a few weeks of intermittent stool incontinence without any reported changes in diet or difficulty tolerating food or fluids by mouth. On the day of presentation, she developed nausea and nonbloody, nonbilious emesis, as well as multiple, loose, nonbloody stools, after becoming acutely ill during lunch at school. There was no reported fever or suspicious food intake. On examination, she was noted to be afebrile, hypotensive, and tachycardic with abdominal tenderness. Her blood work was notable for a marked leukocytosis of 66,000 and a subsequent computed tomography scan of her abdomen was performed. The imaging test identified a large fecaloma with surrounding colonic inflammation concerning for stercoral colitis. The child underwent urgent manual disimpaction and was hospitalized for supportive care with subsequent recovery and return to her normal state of health. Follow-up testing during her hospital stay did not reveal any other infectious or physiologic cause for her constipation and colitis.
Ganglion Cyst of the Temporomandibular Joint Mimicking Auriculotemporal Neuralgia
Authors: Aguilo-Seara Gabriela , Runyan Christopher Michael 
Published: August 2018

Abstract
Ganglion cysts within the temporomandibular joint (TMJ), although uncommon, typically present with swelling, pain, trismus, and difficulty with mastication. The authors report an unusual case of a ganglion cyst in the TMJ of a 52-year-old man who presented with chief complaints of severe headaches and dizziness that had not subsided following treatment with medication, trigger point injections, or sphenopalatine ganglion blocks. The cyst appeared as a nonenhancing, T2 hyperdensity adjacent to the left TMJ condyle on magnetic resonance imaging, supported by the presence of chronic erosion and remodeling of the anterior aspect of the left condylar head on computed tomography. The cyst was surgically removed, and the patient reported that the migraines and accompanying dizziness had ceased 6 months postoperatively. The patient's presentation and improvement following surgery suggest that the location of the cyst in the TMJ and its proximity to the course of the auriculotemporal nerve may have caused auriculotemporal neuralgia, mimicking the symptoms of migraine.
Toward a Consensus on Centralization in Surgery
Authors: Vonlanthen René , Lodge Peter , Barkun Jeffrey S. , Farges Olivier , Rogiers Xavier , Soreide Kjetil , Kehlet Henrik , Reynolds John V. , Käser Samuel A. , Naredi Peter , Borel-Rinkes Inne , Biondo Sebastiano , Pinto-Marques Hugo , Gnant Michael , Nafteux Philippe , Ryska Miroslav , Bechstein Wolf O. , Martel Guillaume , Dimick Justin B. , Krawczyk Marek , Oláh Attila , Pinna Antonio D. , Popescu Irinel , Puolakkainen Pauli A. , Sotiropoulos Georgius C. , Tukiainen Erkki J. , Petrowsky Henrik , Clavien Pierre-Alain 
Published: August 2018

Objectives:
To critically assess centralization policies for highly specialized surgeries in Europe and North America and propose recommendations.

Background/Methods:
Most countries are increasingly forced to maintain quality medicine at a reasonable cost. An all-inclusive perspective, including health care providers, payers, society as a whole and patients, has ubiquitously failed, arguably for different reasons in environments. This special article follows 3 aims: first, analyze health care policies for centralization in different countries, second, analyze how centralization strategies affect patient outcome and other aspects such as medical education and cost, and third, propose recommendations for centralization, which could apply across continents.

Results:
Conflicting interests have led many countries to compromise for a health care system based on factors beyond best patient-oriented care. Centralization has been a common strategy, but modalities vary greatly among countries with no consensus on the minimal requirement for the number of procedures per center or per surgeon. Most national policies are either partially or not implemented. Data overwhelmingly indicate that concentration of complex care or procedures in specialized centers have positive impacts on quality of care and cost. Countries requiring lower threshold numbers for centralization, however, may cause inappropriate expansion of indications, as hospitals struggle to fulfill the criteria. Centralization requires adjustments in training and credentialing of general and specialized surgeons, and patient education.

Conclusion/Recommendations:
There is an obvious need in most areas for effective centralization. Unrestrained, purely “market driven” approaches are deleterious to patients and society. Centralization should not be based solely on minimal number of procedures, but rather on the multidisciplinary treatment of complex diseases including well-trained specialists available around the clock. Audited prospective database with monitoring of quality of care and cost are mandatory.
Total lung capacity without plethysmography
Authors: Adam Ori , Cohen Inon , Yip Wai-Ki , Shiner Robert J. , Calverley Peter , Peles Zachi , LaPrad Adam , Dagan Yoni , Brown Robert , Solway Julian , Fredberg Jeffrey J. 
Published: August 2018

ABSTRACTBackgroundAmong the most basic measures of respiratory function is the total lung capacity (TLC). TLC is the pulmonary gas volume at maximal lung inflation, which is the sum of the volume of gas that can be exhaled –the vital capacity (VC)– and the volume of gas that cannot –the residual volume (RV). Determination of VC requires only spirometry whereas determination of RV or TLC requires body plethysmography, gas dilution or washout, or thoracic imaging, each of which is more complex than spirometry, and none of which is suited to routine office practice, population screening, or community medicine. To fill this gap, we describe here a new approach to determine TLC without plethysmography.MethodsIn a heterogeneous population of 434 volunteers (265 male, 169 female; 201 healthy, 170 with airflow obstruction, and 63 with ventilatory restriction), we determined TLC in the standard fashion using conventional body plethysmography (TLCpleth). In the same individuals, we also determined TLC in a novel fashion using the MiniBox ™ (TLCMB). To obtain TLCMB, population-based data from traditional spirometry together with flow-interruption transients were subjected to data mining and machine-learning to create for each individual subject an unbiased statistical determination of TLC.ResultsFor the combined heterogeneous population, we found TLCpleth= 1.02TLCMB−0.091 L, adjustedr2=0.824. For the heterogeneous population as a whole, and for each subpopulation, TLCMBclosely tracked TLCpleth. For 26 healthy subjects measured on different days, the coefficient of variation for repeated measurements in was 3.3% for TLCplethversus 1.6% for TLCMB.ConclusionsThese results establish the validity and potential utility of a new method for rapid, accurate, and repeatable determination of TLC in a heterogeneous patient population, but without the need of a plethysmograph.
Molecular evolution of non-fertilizing sperm in Lepidoptera suggests minimal direct involvement in sperm competition
Authors: Mongue Andrew J. , Hansen Megan E. , Gu Liuqi , Sorenson Clyde E. , Walters James R. 
Published: August 2018

AbstractSperm are among the most variable cells in nature. Some of this variation results from non-adaptive errors in spermatogenesis, but many species consistently produce multiple sperm morphs, the adaptive significance of which remains unknown. Here, we investigate the evolution of dimorphic sperm in Lepidoptera, the butterflies and moths. Males of this order produce both fertilizing sperm and a secondary, non-fertilizing type that lacks DNA. Previous organismal studies suggested a role for non-fertilizing sperm in sperm competition, but this hypothesis has never been evaluated from a molecular framework. We combined published datasets with new sequencing in two species, the monandrous Carolina sphinx moth and the highly polyandrous monarch butterfly. Based on population genetic analyses, we see evidence for increased adaptive evolution in fertilizing sperm, but only in the polyandrous species. This signal comes primarily from a decrease in non-synonymous polymorphism in sperm proteins compared to the rest of the genome, suggesting stronger purifying selection, consistent with selection via sperm competition. Non-fertilizing sperm proteins, in contrast, do not show an effect of mating system and do not appear to evolve differently from the background genome in either species, arguing against the involvement of non-fertilizing sperm in direct sperm competition. Based on our results and previous work, we suggest that non-fertilizing sperm may be used to delay female remating in these insects and decrease the risk of sperm competition rather than directly affect its outcome.
Biotransformation of 2,4-dinitrotoluene in a phototrophic co-culture of engineeredSynechococcus elongatusandPseudomonas putida
Authors: Fedeson Derek T. , Saake Pia , Calero Patricia , Nikel Pablo Iván , Ducat Daniel C. 
Published: August 2018

AbstractIn contrast to the current paradigm of using microbial monocultures in most biotechnological applications, increasing efforts are being directed towards engineering mixed-species consortia to perform functions that are difficult to program into individual strains. Additionally, the division of labor between specialist species found in natural consortia can lead to increased catalytic efficiency and stability relative to a monoculture or a community composed of generalists. In this work, we have designed a synthetic co-culture for phototrophic degradation of xenobiotics, composed of a cyanobacterium, (Synechococcus elongatusPCC 7942) and a heterotrophic bacterium (Pseudomonas putidaEM173). Cyanobacteria fix CO2through photosynthetic metabolism and secrete sufficient carbohydrates to support the growth and active metabolism ofP. putida, which has been engineered to consume sucrose as the only carbon source and to degrade the environmental pollutant 2,4-dinitrotoluene (2,4-DNT). The synthetic consortium is able to degrade 2,4-DNT with only light and CO2as inputs for the system, and it was stable over time through repeated backdilutions. Furthermore, cycling this consortium through low nitrogen medium promoted the accumulation of polyhydroxyalkanoate (PHA)–an added-value biopolymer–inP. putida, thus highlighting the versatility of this production platform. Altogether, the synthetic consortium allows for simultaneous bioproduction of PHA and remediation of the industrial pollutant 2,4-DNT, using light and CO2as inputs.ImportanceIn this study, we have created an artificial consortium composed of two bacterial species that enables the degradation of the industrially-produced environmental pollutant 2,4-DNT while simultaneously producing PHA bioplastic. In these co-cultures, the photosynthetic cyanobacteria fuel an engineeredP. putidastrain programmed both to use sucrose as a carbon source and to perform the biotransformation of 2,4-DNT. The division of labor in this synthetic co-culture is reminiscent of that commonly observed in microbial communities and represents a proof-of-principle example of how artificial consortia can be employed for bioremediation purposes. Furthermore, this co-culture system enabled the utilization of freshwater sources that could not be utilized in classical agriculture settings, reducing the potential competition of this alternative method of bioproduction with current agricultural practices, as well as remediation of contaminated water streams.
Molecular Characterisation of the Synovial Fluid Microbiome in Rheumatoid Arthritis Patients and Healthy Control Subjects
Authors: Hammad Dargham Bayan Mohsen , Liyanapathirana Veranja , Tonge Daniel Paul 
Published: August 2018

AbstractThe colonisation of specific body sites in contact with the external environment by microorganisms is both well-described and universally accepted, whereas, the existence of microbial evidence in other “classically sterile” locations including the blood, synovial space, and lungs, is a relatively new concept. Increasingly, a role for the microbiome in disease is being considered, and it is therefore necessary to increase our understanding of these. To date, little data support the existence of a “synovial fluid microbiome”.MethodsThe presence and identity of bacterial and fungal DNA in the synovial fluid of rheumatoid arthritis (RA) patients and healthy control subjects was investigated through amplification and sequencing of the bacterial 16S rRNA gene and fungal internal transcribed spacer region 2 respectively. Synovial fluid concentrations of the cytokines IL-6, IL-17A, IL22 and IL-23 were determined by ELISA.ResultsBacterial 16S rRNA genes were detected in 87.5% RA patients, and all healthy control subjects. At the phylum level, the microbiome was predominated byProteobacteria(Control = 83.5%, RA = 79.3%) andFirmicutes(Control = 16.1%, RA = 20.3%), and to a much lesser extent,Actinobacteria(Control = 0.2%, RA = 0.3%) andBacteroidetes(Control = 0.1%, RA = 0.1%). Fungal DNA was identified in 75% RA samples, and 88.8% healthy controls. At the phylum level, synovial fluid was predominated by members of the Basidiomycota (Control = 53.9%, RA = 46.9%) and Ascomycota (Control = 35.1%, RA = 50.8%) phyla. Statistical analysis revealed key taxa that were differentially present or abundant dependent on disease status.ConclusionsThis study reports the presence of a synovial fluid microbiome, and determines that this is modulated by disease status (RA) as are other classical microbiome niches.
Tauopathy in theAPPswe/PS1ΔE9mouse model of familial Alzheimer’s disease
Authors: Metaxas Athanasios , Thygesen Camilla , Kempf Stefan J. , Anzalone Marco , Vaitheeswaran Ramanan , Petersen Sussanne , Landau Anne M. , Audrain Hélène , Teeling Jessica L. , Darvesh Sultan , Brooks David J. , Larsen Martin R. , Finsen Bente 
Published: August 2018

AbstractDespite compelling evidence that the accumulation of amyloid-beta (Aβ) promotes cortical MAPT (tau) aggregation in familial and idiopathic Alzheimer’s disease (AD), murine models of cerebral amyloidosis are not considered to develop tau-associated pathology. The absence of neurofibrillary lesions in amyloidosis mice remains a challenge for the amyloidocentric paradigm of AD pathogenesis. It has resulted in the generation of transgenic mice harboring mutations in theirtaugene, which may be inappropriate for studying a disease with no knownTAUmutations, such as AD. Here, we have usedAPPswe/PS1ΔE9mice to show that tau pathology can develop spontaneously in murine models of familial AD. Tauopathy was abundant around Aβ deposits, with Gallyas- and thioflavin-S-positive perinuclear inclusions accumulating in theAPPswe/PS1ΔE9cortex by 18 months of age. Age-dependent increases in Gallyas signal correlated positively with binding levels of the paired helical filament (PHF) ligand [18F]Flortaucipir, in all brain areas examined. Sarkosyl-insoluble PHFs were visualized by electron microscopy. Tandem mass tag proteomics identified sequences of hyperphosphorylated tau in transgenic mice, along with signs of RNA missplicing, ribosomal dysregulation and disturbed energy metabolism. Human frontal gyrus tissue was used to validate these findings, revealing primarily quantitative differences between the tauopathy observed in AD patient vs. transgenic mouse tissue. Levels oftaumRNA were not different betweenAPPswe/PS1ΔE9and littermate control animals. As physiological levels of endogenous, ‘wild-type’ tau aggregate secondarily to Aβ in transgenic mice, this study demonstrates that amyloidosis is both necessary and sufficient to drive tauopathy in experimental models of familial AD.
Anodal Transcranial Direct Current Stimulation (tDCS) of hMT+ Does Not Affect Motion Perception Learning
Authors: Larcombe Stephanie J. , Kennard Christopher , O’Shea Jacinta , Bridge Holly 
Published: August 2018

AbstractBackgroundHuman visual cortical area hMT+, like its homologue MT in the macaque monkey, has been shown to be particularly selective to visual motion. After damage to the primary visual cortex (V1), patients often exhibit preserved ability to detect moving stimuli, which is associated with neural activity in area hMT+. As an anatomical substrate underlying residual function in the absence of V1, promoting functional plasticity in hMT+ could potentially boost visual performance despite cortical damage.ObjectiveTo establish in healthy participants whether it is possible to use transcranial direct current stimulation (tDCS) over hMT+ to potentiate learning of visual motion direction discrimination.MethodsParticipants were trained daily for five days on a visual motion direction discrimination task. Task difficulty was increased as performance improved, by decreasing the proportion of coherently moving dots, such that participants were always performing at psychophysical threshold. tDCS, either anodal or sham, was applied daily during the 20-minute training session. Task performance was assessed at baseline and at the end of the training period.ResultsAll participants showed improved task performance both during and after training. Contrary to our hypothesis, anodal tDCS did not further improve performance compared to sham stimulation. Bayesian statistics indicated significant evidence in favour of the null hypothesis.ConclusionAnodal tDCS to hMT+ does not enhance visual motion direction discrimination learning in the young healthy visual system.
Identification of subject-specific immunoglobulin alleles from expressed repertoire sequencing data
Authors: Gadala-Maria Daniel , Gidoni Moriah , Marquez Susanna , Heiden Jason A. Vander , Kos Justin T. , Watson Corey T. , O’Connor Kevin C. , Yaari Gur , Kleinstein Steven H. 
Published: August 2018

AbstractThe adaptive immune receptor repertoire (AIRR) contains information on an individuals’ immune past, present and potential in the form of the evolving sequences that encode the B cell receptor (BCR) repertoire. AIRR sequencing (AIRR-seq) studies rely on databases of known BCR germline variable (V), diversity (D) and joining (J) genes to detect somatic mutations in AIRR-seq data via comparison to the best-aligning database alleles. However, it has been shown that these databases are far from complete, leading to systematic misidentification of mutated positions in subsets of sample sequences. We previously presented TIgGER, a computational method to identify subject-specific V gene genotypes, including the presence of novel V gene alleles, directly from AIRR-seq data. However, the original algorithm was unable to detect alleles that differed by more than 5 single nucleotide polymorphisms (SNPs) from a database allele. Here we present and apply an improved version of the TIgGER algorithm which can detect alleles that differ by any number of SNPs from the nearest database allele, and can construct subject-specific genotypes with minimal prior information. TIgGER predictions are validated both computationally (using a leave-one-out strategy) and experimentally (using genomic sequencing), resulting in the addition of three new immunoglobulin heavy chain V (IGHV) gene alleles to the IMGT repertoire. Finally, we develop a Bayesian strategy to provide a confidence estimate associated with genotype calls. All together, these methods allow for much higher accuracy in germline allele assignment, an essential step in AIRR-seq studies.
A deep learning framework for imputing missing values in genomic data
Authors: Qiu Yeping Lina , Zheng Hong , Gevaert Olivier 
Published: August 2018

AbstractMotivationThe presence of missing values is a frequent problem encountered in genomic data analysis. Lost data can be an obstacle to downstream analyses that require complete data matrices. State-of-the-art imputation techniques including Singular Value Decomposition (SVD) and K-Nearest Neighbors (KNN) based methods usually achieve good performances, but are computationally expensive especially for large datasets such as those involved in pan-cancer analysis.ResultsThis study describes a new method: a denoising autoencoder with partial loss (DAPL) as a deep learning based alternative for data imputation. Results on pan-cancer gene expression data and DNA methylation data from over 11,000 samples demonstrate significant improvement over standard denoising autoencoder for both data missing-at-random cases with a range of missing percentages, and missing-not-at-random cases based on expression level and GC-content. We discuss the advantages of DAPL over traditional imputation methods and show that it achieves comparable or better performance with less computational burden.Availabilityhttps://github.com/gevaertlab/DAPLContactogevaert@stanford.edu
Exploration and Exploitation in Organizational Learning: A Critical Application of the 4I Model
Authors: Nielsen Jeppe Agger , Mathiassen Lars , Hansen Anne Mette 
Published: August 2018

AbstractWhile it is broadly agreed that managers face tensions between exploration of new possibilities and exploitation of existing certainties, there are open questions related to how they can recognize these tensions and distribute resources between the two forms of learning across time and space. The heavily cited 4I Model made significant progress towards addressing these questions, based on the idea that exploration and exploitation unfold differently across organizational levels, with exploration as feed‐forward from the individual towards the organizational level, and exploitation as feedback from the organizational towards the individual level. The authors’ critical application of the model questions this idea, suggesting that exploration and exploitation unfold through similar patterns from the individual level towards the organizational level with iterative feedback loops. This repositioning of the 4I Model affords a detailed understanding of both exploration and exploitation processes as well as the temporal and spatial interactions between them. To demonstrate the utility of this theorizing and to add new insights to the process literature on organizational learning, the authors rely on a longitudinal case study of how a management team engaged in technology‐enabled strategic renewal in a Danish local government organization.
An Incomplete Victory: The Implications of QT v Director of Immigration for the Protection of Gay Rights in Hong Kong
Authors: Wong Kai Yeung 
Published: August 2018

AbstractQT v Director of Immigration is the most important decision on gay rights in Hong Kong since the unequal ages of consent between heterosexuals and homosexuals were held to be unconstitutional 10 years ago. The Court of Appeal of Hong Kong affirmed the right of same‐sex couples married or in a civil partnership overseas to be treated on an equal basis with married heterosexual couples. This note considers the strengths and shortcomings of the Court of Appeal's reasoning, in terms of its potential significance both to the rights of sexual minorities and to the wider protection of human rights by means of the common law.