bluuebunny/crossref_metadata_2025_split huggingface dataset:
An intrinsic association between olfactory identification and spatial memory in humans
Authors: Dahmani Louisa , Patel Raihaan M. , Yang Yiling , Chakravarty M. Mallar , Fellows Lesley K. , Bohbot Véronique D. 
Published: October 2018

AbstractIt was recently proposed that olfaction evolved to aid navigation. Consistent with this hypothesis, olfactory identification and spatial memory are linked to overlapping brain areas which include the orbitofrontal cortex and hippocampus. However, the relationship between these two processes has never been specifically investigated. Here, we show that olfactory identification covaries with spatial memory in humans. We also found that the cortical thickness of the left medial orbitofrontal cortex, and the volume of the right hippocampus, predict both olfactory identification and spatial memory. Finally, we demonstrate deficits in both olfactory identification and spatial memory in patients with lesions of the medial orbitofrontal cortex. Our findings reveal an intrinsic relationship between olfaction and spatial memory that is supported by a shared reliance on the hippocampus and medial orbitofrontal cortex. This relationship may find its roots in the parallel evolution of the olfactory and hippocampal systems.
Interface-mediated hygroelectric generator with an output voltage approaching 1.5 volts
Authors: Huang Yaxin , Cheng Huhu , Yang Ce , Zhang Panpan , Liao Qihua , Yao Houze , Shi Gaoquan , Qu Liangti 
Published: October 2018

AbstractHygroelectricity is proposed as a means to produce electric power from air by absorbing gaseous or vaporous water molecules, which are ubiquitous in the atmosphere. Here, using a synergy between a hygroscopic bulk graphene oxide with a heterogeneous structure and interface mediation between electrodes/materials with Schottky junctions, we develop a high-performance hygroelectric generator unit with an output voltage approaching 1.5 V. High voltage (e.g., 18 V with 15 units) can be easily reached by simply scaling up the number of hygroelectric generator units in series, enough to drive commercial electronic devices. This work provides insight for the design and development of hygroelectric generators that may promote the efficient conversion of potential energy in the environmental atmosphere to electricity for practical applications.
Genetic signature to provide robust risk assessment of psoriatic arthritis development in psoriasis patients
Authors: Patrick Matthew T. , Stuart Philip E. , Raja Kalpana , Gudjonsson Johann E. , Tejasvi Trilokraj , Yang Jingjing , Chandran Vinod , Das Sayantan , Callis-Duffin Kristina , Ellinghaus Eva , Enerbäck Charlotta , Esko Tõnu , Franke Andre , Kang Hyun M. , Krueger Gerald G. , Lim Henry W. , Rahman Proton , Rosen Cheryl F. , Weidinger Stephan , Weichenthal Michael , Wen Xiaoquan , Voorhees John J. , Abecasis Gonçalo R. , Gladman Dafna D. , Nair Rajan P. , Elder James T. , Tsoi Lam C. 
Published: October 2018

AbstractPsoriatic arthritis (PsA) is a complex chronic musculoskeletal condition that occurs in ~30% of psoriasis patients. Currently, no systematic strategy is available that utilizes the differences in genetic architecture between PsA and cutaneous-only psoriasis (PsC) to assess PsA risk before symptoms appear. Here, we introduce a computational pipeline for predicting PsA among psoriasis patients using data from six cohorts with >7000 genotyped PsA and PsC patients. We identify 9 new loci for psoriasis or its subtypes and achieve 0.82 area under the receiver operator curve in distinguishing PsA vs. PsC when using 200 genetic markers. Among the top 5% of our PsA prediction we achieve >90% precision with 100% specificity and 16% recall for predicting PsA among psoriatic patients, using conditional inference forest or shrinkage discriminant analysis. Combining statistical and machine-learning techniques, we show that the underlying genetic differences between psoriasis subtypes can be used for individualized subtype risk assessment.
Physical descriptor for the Gibbs energy of inorganic crystalline solids and temperature-dependent materials chemistry
Authors: Bartel Christopher J. , Millican Samantha L. , Deml Ann M. , Rumptz John R. , Tumas William , Weimer Alan W. , Lany Stephan , Stevanović Vladan , Musgrave Charles B. , Holder Aaron M. 
Published: October 2018

AbstractThe Gibbs energy, G, determines the equilibrium conditions of chemical reactions and materials stability. Despite this fundamental and ubiquitous role, G has been tabulated for only a small fraction of known inorganic compounds, impeding a comprehensive perspective on the effects of temperature and composition on materials stability and synthesizability. Here, we use the SISSO (sure independence screening and sparsifying operator) approach to identify a simple and accurate descriptor to predict G for stoichiometric inorganic compounds with ~50 meV atom−1 (~1 kcal mol−1) resolution, and with minimal computational cost, for temperatures ranging from 300–1800 K. We then apply this descriptor to ~30,000 known materials curated from the Inorganic Crystal Structure Database (ICSD). Using the resulting predicted thermochemical data, we generate thousands of temperature-dependent phase diagrams to provide insights into the effects of temperature and composition on materials synthesizability and stability and to establish the temperature-dependent scale of metastability for inorganic compounds.
A mechanoelectrical mechanism for detection of sound envelopes in the hearing organ
Authors: Nuttall Alfred L. , Ricci Anthony J. , Burwood George , Harte James M. , Stenfelt Stefan , Cayé-Thomasen Per , Ren Tianying , Ramamoorthy Sripriya , Zhang Yuan , Wilson Teresa , Lunner Thomas , Moore Brian C. J. , Fridberger Anders 
Published: October 2018

AbstractTo understand speech, the slowly varying outline, or envelope, of the acoustic stimulus is used to distinguish words. A small amount of information about the envelope is sufficient for speech recognition, but the mechanism used by the auditory system to extract the envelope is not known. Several different theories have been proposed, including envelope detection by auditory nerve dendrites as well as various mechanisms involving the sensory hair cells. We used recordings from human and animal inner ears to show that the dominant mechanism for envelope detection is distortion introduced by mechanoelectrical transduction channels. This electrical distortion, which is not apparent in the sound-evoked vibrations of the basilar membrane, tracks the envelope, excites the auditory nerve, and transmits information about the shape of the envelope to the brain.
Unravelling the structure of glycosyl cations via cold-ion infrared spectroscopy
Authors: Mucha Eike , Marianski Mateusz , Xu Fei-Fei , Thomas Daniel A. , Meijer Gerard , von Helden Gert , Seeberger Peter H. , Pagel Kevin 
Published: October 2018

AbstractGlycosyl cations are the key intermediates during the glycosylation reaction that covalently links building blocks during the synthetic assembly of carbohydrates. The exact structure of these ions remained elusive due to their transient and short-lived nature. Structural insights into the intermediate would improve our understanding of the reaction mechanism of glycosidic bond formation. Here, we report an in-depth structural analysis of glycosyl cations using a combination of cold-ion infrared spectroscopy and first-principles theory. Participating C2 protective groups form indeed a covalent bond with the anomeric carbon that leads to C1-bridged acetoxonium-type structures. The resulting bicyclic structure strongly distorts the ring, which leads to a unique conformation for each individual monosaccharide. This gain in mechanistic understanding fundamentally impacts glycosynthesis and will allow to tailor building blocks and reaction conditions in the future.
Optimizing the utilization of aluminum adjuvants in vaccines: you might just get what you want
Authors: HogenEsch Harm , O’Hagan Derek T. , Fox Christopher B. 
Published: October 2018

AbstractAluminum-containing adjuvants have been used for over 90 years to enhance the immune response to vaccines. Recent work has significantly advanced our understanding of the physical, chemical, and biological properties of these adjuvants, offering key insights on underlying mechanisms. Given the long-term success of aluminum adjuvants, we believe that they should continue to represent the “gold standard” against which all new adjuvants should be compared. New vaccine candidates that require adjuvants to induce a protective immune responses should first be evaluated with aluminum adjuvants before other more experimental approaches are considered, since use of established adjuvants would facilitate both clinical development and the regulatory pathway. However, the continued use of aluminum adjuvants requires an appreciation of their complexities, in combination with access to the necessary expertise to optimize vaccine formulations. In this article, we will review the properties of aluminum adjuvants and highlight those elements that are critical to optimize vaccine performance. We will discuss how other components (excipients, TLR ligands, etc.) can affect the interaction between adjuvants and antigens, and impact the potency of vaccines. This review provides a resource and guide, which will ultimately contribute to the successful development of newer, more effective and safer vaccines.
Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells
Authors: Baaske Julia , Gonschorek Patrick , Engesser Raphael , Dominguez-Monedero Alazne , Raute Katrin , Fischbach Patrick , Müller Konrad , Cachat Elise , Schamel Wolfgang W. A. , Minguet Susana , Davies Jamie A. , Timmer Jens , Weber Wilfried , Zurbriggen Matias D. 
Published: October 2018

AbstractOptogenetic switches are emerging molecular tools for studying cellular processes as they offer higher spatiotemporal and quantitative precision than classical, chemical-based switches. Light-controllable gene expression systems designed to upregulate protein expression levels meanwhile show performances superior to their chemical-based counterparts. However, systems to reduce protein levels with similar efficiency are lagging behind. Here, we present a novel two-component, blue light-responsive optogenetic OFF switch (‘Blue-OFF’), which enables a rapid and quantitative down-regulation of a protein upon illumination. Blue-OFF combines the first light responsive repressor KRAB-EL222 with the protein degradation module B-LID (blue light-inducible degradation domain) to simultaneously control gene expression and protein stability with a single wavelength. Blue-OFF thus outperforms current optogenetic systems for controlling protein levels. The system is described by a mathematical model which aids in the choice of experimental conditions such as light intensity and illumination regime to obtain the desired outcome. This approach represents an advancement of dual-controlled optogenetic systems in which multiple photosensory modules operate synergistically. As exemplified here for the control of apoptosis in mammalian cell culture, the approach opens up novel perspectives in fundamental research and applications such as tissue engineering.
Temperature-mediated invocation of the vacuum state for switchable ultrawide-angle and broadband deflection
Authors: Serebryannikov Andriy E. , Lakhtakia Akhlesh , Aalizadeh Majid , Ozbay Ekmel , Vandenbosch Guy A. E. 
Published: October 2018

AbstractTemperature-mediated appearance and disappearance of a deflection grating in a diffracting structure is possible by employing InSb as the grating material. InSb transits from the dielectric state to the plasmonic state in the terahertz regime as the temperature increases, this transition being reversible. An intermediate state is the vacuum state in which the real part of the relative permittivity of InSb equals unity while the imaginary part is much smaller. Then the grating virtually disappears, deflection being impossible as only specular reflection can occur. This ON/OFF switching of deflection and relevant angular filtering are realizable over wide ranges of frequency and incidence angle by a temperature change of as low as 20 K. The vacuum state of InSb invoked for ON/OFF switching of deflection and relevant angular filtering can also be obtained for thermally tunable materials other than InSb as well as by using non-thermal mechanisms.
Porcine Reproductive and Respiratory Syndrome Virus strains with Higher Virulence Cause Marked Protein Profile Changes in MARC-145 Cells
Authors: Chen Zhi , Liu Shaoning , Zhang Shujin , Zhang Yuyu , Yu Jiang , Sun Wenbo , Chen Lei , Du Yijun , Wang Jinbao , Li Yubao , Wu Jiaqiang 
Published: October 2018

AbstractPorcine reproductive and respiratory syndrome is an infectious disease that causes serious economic losses to the swine industry worldwide. To better understand the pathogenesis of the porcine reproductive and respiratory syndrome virus (PRRSV), three PRRSV strains with different molecular markers and virulence were used to infect MARC-145 cells. A total of 1804 proteins were identified, and 233 altered proteins and 72 signaling pathways involved in the proteomic profiling of virus-infected MARC-145 cells increased with the virulence of the PRRSV strain. The three types of viral strains shared a common pathway—the electron transport reaction in mitochondria—in the infected-MARC-145 cells. Moreover, the antisense pathway was the most variable of all significant signaling pathways for the highly virulent SX-1 strain, indicating that this unique pathway may be connected to the high virulence of the SX-1 strain. Our study is the first attempt to provide a proteome profile of MARC-145 cells infected with PRRSV strains with different virulence, and these findings will facilitate a deep understanding of the interactions between this virus and its host.
Circadian monitoring as an aging predictor
Authors: Martinez-Nicolas A. , Madrid J. A. , García F. J. , Campos M. , Moreno-Casbas M. T. , Almaida-Pagán P. F. , Lucas-Sánchez A. , Rol M. A. 
Published: October 2018

AbstractThe ageing process is associated with sleep and circadian rhythm (SCR) frailty, as well as greater sensitivity to chronodisruption. This is essentially due to reduced day/night contrast, decreased sensitivity to light, napping and a more sedentary lifestyle. Thus, the aim of this study is to develop an algorithm to identify a SCR phenotype as belonging to young or aged subjects. To do this, 44 young and 44 aged subjects were recruited, and their distal skin temperature (DST), activity, body position, light, environmental temperature and the integrated variable TAP rhythms were recorded under free-living conditions for five consecutive workdays. Each variable yielded an individual decision tree to differentiate between young and elderly subjects (DST, activity, position, light, environmental temperature and TAP), with agreement rates of between 76.1% (light) and 92% (TAP). These decision trees were combined into a unique decision tree that reached an agreement rate of 95.3% (4 errors out of 88, all of them around the cut-off point). Age-related SCR changes were very significant, thus allowing to discriminate accurately between young and aged people when implemented in decision trees. This is useful to identify chronodisrupted populations that could benefit from chronoenhancement strategies.
Naturally Occurring Variations Modulate the Activity of the HPV33 Early Promoter and its Affinity for the E2 Transcription Factor
Authors: Alvarez Jennifer , Gagnon David , Coutlée François , Archambault Jacques 
Published: October 2018

AbstractThe human papillomavirus (HPV) Long Control Region (LCR) encompasses the early promoter (EP) that drives transcription of the E6 and E7 oncogenes in keratinocytes and HPV-associated cancers. In this study, the transcriptional activities of the HPV33 EP from the prototype LCR and from eight variants representative of the worldwide diversity of the virus were examined in primary human keratinocytes (PHK) and in the HeLa cervical carcinoma cell line by luciferase reporter-gene assays. Remarkably, the two variations with the greatest effect on the EP in PHK were C7732G and a 79-bp deletion that were associated with high-grade cervical lesions and persistent infections, respectively, in epidemiological studies. In contrast, the three variations most active in HeLa cells were C7537A, A7874C and A7879G. A7874C, which lies within an E2-binding sequence, is also shown to increase the activity and binding of E2 at this site. Collectively, these results indicate that naturally-occurring variations affect the HPV33 EP differentially in PHK than in cancer cells and, furthermore, that they can also alter its regulation by E2. These findings provide a molecular basis for rationalizing the results of previous epidemiological studies and for understanding the contribution of LCR polymorphisms to the oncogenicity and persistence of HPV33 infections.
AIMP3 Deletion Induces Acute Radiation Syndrome-like Phenotype in Mice
Authors: Kim Doyeun , Kim Sunmi , Oh Youngsun , Park Songhwa , Jeon Yoon , Kim Hongtae , Lee Ho , Kim Sunghoon 
Published: October 2018

AbstractGenomes are mostly protected from constant DNA-damaging threats, either internal or external, which ultimately sustain the organism. Herein, we report that AIMP3, a previously demonstrated tumour suppressor, plays an essential role in maintaining genome integrity in adult mice. Upon induction of the temporal systemic deletion of AIMP3 by tamoxifen in adult mice, the animals developed an acute radiation syndrome-like phenotype, typified by scleroderma, hypotrophy of haematopoietic cells and organs, and intestinal failure. Induction of γH2AX, an early marker of DNA double-strand breaks, was observed in the spleen, intestine, and the highly replicating embryonic cortex. In addition, sub-lethal irradiation of AIMP3 mKO mice dramatically affected organ damage and survival. Using isolated MEFs from conditional KO mice or AIMP3 knockdown cells, we confirmed the presence of spontaneously occurring DNA double-strand breaks by COMET assay and γH2AX induction. Furthermore, γH2AX removal was delayed, and homologous DNA repair activity was significantly reduced. Reduction of RPA foci formation and subsequent Rad51 foci formation probably underlie the significant reduction in homologous recombination activity in the absence of AIMP3. Together, our data demonstrate that AIMP3 plays a role in genome stability through the DNA repair process.
Using long term mortality to determine which perioperative risk factors of mortality following hip and knee replacement may be causal
Authors: Hunt Linda P. , Whitehouse Michael R. , Howard Peter W. , Ben-Shlomo Yoav , Blom Ashley W. 
Published: October 2018

AbstractObservational studies have identified surgical factors that are associated with a reduced risk of mortality after joint replacement. It is not clear whether these are causal or reflect patient selection. Data on the first primary hip (n = 424,156) and knee replacements (n = 469,989) performed for osteoarthritis in the National Joint Registry were analysed. Flexible parametric survival modelling was used to determine if risk factors for mortality in the perioperative period persisted. To explore selection bias, standardised mortality ratios were calculated for all-cause, respiratory and smoking related cancer mortality using population rates. Selection was apparent for hip resurfacing, combined spinal and general anaesthetic and unicondylar knee implants; reduced mortality was observed for many years for both all and other causes of mortality with a waning effect. Mechanical thromboprophylaxis was also suggestive of selection although patients receiving aspirin had sustained reduced mortality, possibly due to to a cardioprotective effect. Posterior approach for hips was ambiguous with a possible causal component. Spinal anaesthesia was suggestive of a causal effect. We are reliant on observational data when it is not feasible to undertake randomised trials. Our approach of looking at long term mortality risks for perioperative interventions provides further insights to differentiate causal interventions from selection. We recommend the use of aspirin chemothromboprophylaxis, the posterior approach and spinal anaesthetic in total hip replacement due to the apparent causal effect on reduced mortality.
Peptide-oligonucleotide conjugates exhibiting pyrimidine-X cleavage specificity efficiently silence miRNA target acting synergistically with RNase H
Authors: Patutina O. A. , Bazhenov M. A. , Miroshnichenko S. K. , Mironova N. L. , Pyshnyi D. V. , Vlassov V. V. , Zenkova M. A. 
Published: October 2018

AbstractTaking into account the important role of miRNA in carcinogenesis, oncogenic miRNAs are attractive molecules for gene-targeted therapy. Here, we developed a novel series of peptide-oligonucleotide conjugates exhibiting ribonuclease activity targeted to highly oncogenic miRNAs miR-21 and miR-17. When designing the conjugates, we enhanced both nuclease resistance of the targeted oligodeoxyribonucleotide by introducing at its 3′-end mini-hairpin structure displaying high thermostability and robustness against nuclease digestion and the efficiency of its functioning by attachment of the catalytic construction (amide)NH2-Gly(ArgLeu)4-TCAA displaying ribonuclease activity to its 5′-end. Designed miRNases efficiently cleaved miRNA targets, exhibiting Pyr-X specificity, and cleavage specificity had strong dependence on the miRNA sequence in the site of peptide location. In vitro, designed miRNases do not prevent cleavage of miRNA bound with the conjugate by RNase H, and more than an 11-fold enhancement of miRNA cleavage by the conjugate is observed in the presence of RNase H. In murine melanoma cells, miRNase silences mmu-miR-17 with very high efficiency as a result of miR-17 cleavage by miRNase and by recruited RNase H. Thus, miRNases provide a system of double attack of the miRNA molecules, significantly increasing the efficiency of miRNA downregulation in the cells in comparison with antisense oligonucleotide.
Febrile Temperature Elevates the Expression of Phosphatidylserine on Plasmodium falciparum (FCR3CSA) Infected Red Blood Cell Surface Leading to Increased Cytoadhesion
Authors: Zhang Rou , Chandramohanadas Rajesh , Lim Chwee Teck , Dao Ming 
Published: October 2018

AbstractDuring the asexual intra-erythrocytic cycle, Plasmodium (P.) falciparum exports parasitic proteins to the surface of infected red blood cells (iRBCs) facilitating its cytoadhesion to various endothelial host receptors. This adhesive behavior is a critical contributor towards disease manifestation. However, little is known about the influence of recurring elevated temperature – a common symptom of the malaria infection – on the adhesive properties of iRBCs to endothelial receptors. To address this, we performed dual-micropipette step-pressure technique between P. falciparum (strain FCR3CSA) iRBCs and Chinese Hamster Ovary cells expressing Chondroitin sulfate A (CHO-CSA) after transient iRBCs incubation at febrile temperatures which revealed increase in adhesion parameters. Furthermore, flow cytometry analysis revealed an increase in phosphatidylserine (PS) expression on the iRBC surface following exposure to febrile temperature. The adhesion between iRBCs and CHO-CSA cells was remarkably reduced in presence of soluble Annexin V, indicating the mediation of PS on the adhesion events. Our results suggest that elevated PS recruitment on iRBC under thermally stressed conditions contributes to the increased adhesive behavior of iRBCs CSA-binding phenotype to CHO-CSA.