bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical value of MLH1-negative circulating tumor cells in lung cancer patients
Authors: Liang Jin-Yan , Yang Qi-Fan , Zeng Yu-Lan , Liu Yang-Yang , Liu Yu-Ting , Gu Fei-Fei , Hu Yue , Zhang Kai , Zhong Hao , Liu Li 
Published: June 2019

Abstract
Circulating tumor cells (CTCs) serve as valuable biomarkers. However, MutL homolog 1 (MLH1)-negative CTCs and their clinical significance in lung cancer are nearly unknown.
Here, bioinformatic analysis of MLH1 expression and its clinical significance was conducted using the Oncomine, Ualcan, and Kaplan–Meier plotter websites. Size-based isolation and RNA in situ hybridization assays were used to identify CTCs and evaluate MLH1 and mesenchymal marker expression in CTCs. MLH1 was downregulated in lung cancer patients. Patients with lower MLH1 expression levels had worse prognoses. In a cohort of 32 randomly selected patients with lung cancer, the patients with poorer treatment responses had more MLH1-negative CTCs. The total CTCs, MLH1-negative CTCs and mesenchymal markers-expressing CTCs levels were negatively correlated with prognosis in the lung cancer patients.
Our data showed the clinical significance of MLH1 expression in lung cancer tissues. The characterization and numeration of CTCs based on the expression of MLH1 and mesenchymal markers may be a convenient approach for predicting treatment response and prognosis in lung cancer.
Communicating health risk in Southeast Nigeria
Authors: Wogu Joseph Oluchukwu , Chukwu Christiana Ogeri , Orekyeh Emeka Samuel Shawn , Anorue Luke Ifeanyi , Nwokedi Ozioma , Chukwu Loveth Chinyere 
Published: June 2019

Abstract
The purpose of this study is to investigate the media coverage of viral hepatitis in Southeast Nigeria and the implication it has on health communication. This study was conducted with the aim of determining the level of awareness of viral hepatitis disease in Southeast Nigeria; and the extent of media coverage of hepatitis disease.
Cross-sectional survey study was carried out in the 5 states of Southeast Nigeria while structured questionnaire was used to generate data. A representative sample of 500 respondents was selected from the capital cities of the 5 states in the Southeast zone of Nigeria. The data generated were analyzed using mean, percentage (%), and analysis of variance tests of between-subjects effects in SPSS, version 20. Results were considered significant at P ≤ .05.
Even though majority of the people are consumers of media products, only a few of the respondents were aware of viral hepatitis, its symptoms, mode of transmission, and curative measures. Inappropriate timing and scanty or limited scheduling of media health programs were significant hindrances to media campaign against viral hepatitis in Southeast Nigeria.
Regular media campaign programs against the disease are required to build more awareness as well as appropriate timing of media programs such as Sunday evenings and news hours, and media interactive forum with phone-in programs. Further research on the nature and impact of funding, support, and policy on media campaigns on this health risk are also recommended.
Meta-analysis on allopurinol preventive intervention on contrast-induced acute kidney injury with random controlled trials
Authors: Ma Guang , Wang Guoliang , Xiao Dongbin , Teng Wei , Hui Xuezhi , Ma Guang 
Published: June 2019

Abstract

Objectives:
The objective of this meta-analysis on randomized controlled trials is to evaluate whether the administration of allopurinol with or without hydration will reduce contrast-induced acute kidney injury (CI-AKI) in patients undergoing contrast exposure.

Background:
The efficacy of allopurinol in the prevention of CI-AKI after cardiac catheterization and percutaneous coronary intervention (PCI) is significantly related to the heterogeneous results.

Methods:
Two investigators independently searched MEDLINE, EMBASE, the Cochrane Controlled Trials Registry, the China Wanfang Data, the China Biological Medicine Database and the China National Knowledge Infrastructure (CNKI) databases for randomized controlled trials (RCTs) comparing allopurinol with placebo or no allopurinol for the prevention of CI-AKI in patients from their inception to July 31, 2018. The primary outcome was the incidence of CI-AKI, and the secondary outcomes were the differences of serum creatinine (Scr), blood urea nitrogen (BUN), uric acid (UA), and estimated glomerular filtration rate (eGFR) levels between groups after contrast media exposure. We used fixed-effects or random-effects models according to I2 statistics. The meta-analytic procedures were completed by Review Manager, version 5.3.

Achievements:
Eight random controlled trials with 1141 patients were included for this analysis. Compared with the control, allopurinol was associated with a reduced risk of CI-AKI (Relatives Risk (RR) 0.39, 95% confidence interval [CI] 0.20,0.74, P = .004) and only a intend for decrease a post-procedure uric acid levels compared with the controlled ones at 48 hours (standardized mean difference (SMD) −0.72, 95% CI −1.44, 0.01, P = .05). But the difference of post-procedure uric acid levels was not statistically significant in allopurinol groups compared with controlled groups. There were lower post-procedure Scr and BUN levels in allopurinol groups than those in controlled groups (SMD −0.50, 95% CI −0.79,−0.21, P = .0009; SMD −0.40, 95% CI −0.60,−0.20, P < .0001;respectively). There were higher post-procedure eGFR levels in allopurinol groups than those in controlled groups (SMD 0.65, 95% CI 0.48, 0.83, P < .0001).

Conclusion:
The main findings of this meta-analysis are focus on allopurinol may cause reduces in the incidence of CI-AKI in patients undergoing interventional coronary procedures. Further researches are still required for confirmation.
Combination of three-dimensional arterial spin labeling and stretched-exponential model in grading of gliomas
Authors: Qu Yuan , Zhou Lisui , Jiang Jie , Quan Guangnan , Wei Xiaocheng 
Published: June 2019

AbstractTo evaluate the diagnostic value of combining 3D arterial spin labeling (ASL) and stretched-exponential diffusion model in grading of gliomas.A total of 72 patients with histo-pathology proved gliomas (34 low-grade, 38 high-grade) were included in this study. 3D ASL and multi-bdiffusion weighted imaging (DWI) images were retrospectively analyzed. The ASL and DWI parameters—tumor blood flow (TBF), distributed diffusion coefficient (DDC), and diffusion heterogeneityαwere compared between high-grade and low-grade groups andP < .05 was regarded as statistically significant. TBF was also normalized to the corresponding values in contralateral mirror regions of interest (ROI) (M-TBF), normal grey matter (G-TBF), and white matter (W-TBF) and were compared between high and low-grade tumors.TBF values were significantly higher in high-grade gliomas (P < .001). In stretched-exponential model, theαvalue of low-grade gliomas showed significant higher than high-grade gliomas group (P < .001), but there was no difference of DDC (P > .05). When TBF values were normalized to contralateral mirror ROI, normal grey matter and white matter, G-TBF showed the highest sensitivity and specificity for differentiation high-grade and low-grade gliomas. The area under area under curve (AUC) of G-TBF andαfor glioma grading were 0.926 and 0.892, respectively. The area under AUC of the G-TBF combination withαwas 0.960 and corresponding sensitivity and specificity were 94.1% and 98.7%.The combination of 3D ASL and stretched-exponential model parameters can be used to differentiate high-grade and low-grade gliomas. Combination G-TBF andαvalue can obtain best diagnostic performance.
Exercise vs metformin for gestational diabetes mellitus
Authors: Pascual-Morena Carlos , Martínez-Vizcaíno Vicente , Álvarez-Bueno Celia , Pozuelo-Carrascosa Diana P. , Notario-Pacheco Blanca , Saz-Lara Alicia , Fernández-Rodriguez Rubén , Cavero-Redondo Iván 
Published: June 2019

Abstract

Introduction:
The purpose of this protocol is to provide a network meta-analysis methodology that compares the effects of metformin and physical exercise in the prevention and treatment of gestational diabetes mellitus (GDM) and its associated fetal and maternal morbidity.

Methods and analysis:
This protocol conforms to the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) and the recommendations of the Cochrane Collaboration Handbook. An electronic search will be conducted in MEDLINE, EMBASE, Web of Science and the Cochrane Library, from the inception until July 2019. There will be no language restrictions. The Cochrane Collaboration tool for assessing risk of bias (RoB2) and the quality assessment tool for quantitative studies will be used. The Grading of Recommendations, Assessment, Development and Evaluation scale will be used to evaluate the strength of the evidence. A Bayesian network meta-analysis will be carried out, which allows direct and indirect comparison of the interventions, for the risk of GDM, prematurity, caesarean section, macrosomia, hypertensive disorders, insulin requirement, and differences in basal glucose, maternal weight, and weight of the newborn.

Discussion:
With this protocol, a methodology is established that resolves the limitations of previous meta-analysis. It will be possible to determine the difference of effect between physical exercise and metformin in the main outcomes of the GDM, as well as the type and intensity of the exercise, and the dose of metformin, more effective.

Ethics and dissemination:
The data included in the network meta-analysis will be obtained from trials that meet accepted ethical standards and the Declaration of Helsinki. The results will be published in a peer-reviewed journal. The evidence obtained could be included in the guidelines of clinical practice in pregnancy.

Strengths and limitations:
A comprehensive methodology is established for the analysis, through network meta-analysis, of the comparative efficacy of metformin and physical exercise in gestational diabetes mellitus. The results obtained could help medical professionals by establishing the best evidence-based interventions which may be recommended for these population groups.

Registration number:
PROSPERO CRD42019121715
Therapies of varicose veins
Authors: Ding Jie , Mu XiaoFei , Yuan Yuan , Tang LiYao , Wei KongXi , Zhao XiYun , Qing LiNa , Liu Cai 
Published: June 2019

Abstract

Background:
Many pairwise meta-analyses (MAs) related to therapies of varicose veins have been published, but their reporting and methodological quality remain unclear. The present study was designed to assess the overall quality of pairwise MAs related to therapies of varicose veins.

Methods:
We will systematically search 4 electronic databases, including PubMed, EMBASE, Cochrane Library, Chinese Biomedical Database, to identify pairwise MAs related to therapies of varicose veins. The search time-span was set from inception to March 2019. The pairwise MAs related to therapies of varicose veins will be included in our overview. The reporting and methodological quality of included MAs will be assessed using preferred reporting items for systematic review and meta-analysis and a measurement tool to assess systematic reviews 2, respectively. Meanwhile, we will extract some general characteristics of included MAs, including first author; published year, journal, sample size, number of studies, number of randomized controlled trials and intervention details, and so on. All literatures screening, quality assessment, and data extraction will be independently completed by 2 of all reviewers, and any disagreement will be resolved by discussion. Besides, an increasingly popular method – evidence mapping, will be used to present the whole evidence landscape related to therapies of varicose veins. The assessment results will be presented as percentage and event/total. The Excel 2016 will be used to manage and analyze data.

Results:
The results of the overview will be submitted to a peer-reviewed journal for publication.

Conclusion:
This overview will summarize the overall reporting and methodological quality related to therapies of varicose veins.

PROSPERO registration number:
CRD42019126722.
Nutritional supplementation of vitamin A and health-related outcomes in patients with multiple sclerosis
Authors: Nunes Ana Clara de França , Piuvezam Grasiela 
Published: June 2019

Abstract

Background:
Multiple sclerosis (MS) is a chronic immune mediated disease which affects the central nervous system (CNS), having a substantial financial, functional, and quality of life (QOL) impact on these people. The vitamin A supplementation has been studied as a therapeutic possibility for in MS. Therefore, the objective of this protocol is to build an outline for a future systematic review, which will provide up-to-date available evidence about the clinical impact of nutritional supplementation of vitamin A in the outcomes related to the symptoms in patients with this pathology.

Methods:
The search will be performed in the following databases: PubMed, Embase, Scopus, cinahl, Scielo, Web of Science, the Cochrane Library and Science Direct, randomized clinical trials published until May 2019 that evaluate the relationship of the supplementation of vitamin A and health-related outcomes in patients with MS will be included. Preferred Reporting Items for Systematic review and Meta-Analysis Protocols (PRISMA-P) will be used to outline the protocol, and PRISMA to the systematic review. Undergraduate handbook of quality of evidence and strength of recommendations for decision making in health (GRADE) will be used to assess the quality of evidence and the strength of the recommendation, and the JADAD scale to assess the internal validity of selected studies. For the extraction of all the data found a database in Microsoft Excel will be created. For the summary of the findings the Cochrane Collaboration Handbook recommendations will be used, and for the meta-analysis standard statistical techniques the RevMan software will be used.

Results:
In this study, we hope to find a considerable number of articles presenting evidence about the effectiveness of vitamin A supplementation in patients with MS.

Conclusion:
Currently, many lines of evidence have been produced when it comes to the use of food supplements. This systematic review proposal might provide recent, important, and trusted information for better treatment of patients.

Record of systematic review:
This review was recorded in the International Register of Prospective Systematic Reviews (PROSPERO) on the January 30, 2019 (registration: CRD42019121757). Available at: http://www.crd.york.ac.uk/PROSPERO/display_record.php?ID=CRD42019121757.
Application of a Tabu search-based Bayesian network in identifying factors related to hypertension
Authors: Pan Jinhua , Rao Huaxiang , Zhang Xuelei , Li Wenhan , Wei Zhen , Zhang Zhuang , Ren Hao , Song Weimei , Hou Yuying , Qiu Lixia 
Published: June 2019

Abstract
The study aimed to study the related factors of hypertension using multivariate logistic regression analysis and tabu search-based Bayesian Networks (BNs). A cluster random sampling method was adopted to obtain samples of the general population aged 15 years or above. Multivariate logistic regression analysis indicated that gender, age, cultural level, body mass index (BMI), central obesity, drinking, diabetes mellitus, Myocardial infarction, Coronary heart disease, Stroke are associated with hypertension. While BNs found connections between those related factors and hypertension were established by complex network structure, age, smoking, occupation, cultural level, BMI, central obesity, drinking, diabetes mellitus, myocardial infarction, coronary heart disease, nephropathy, stroke were direct connection with hypertension, gender was indirectly linked to hypertension through drinking. The results showed that BNs can not only find out the correlative factors of hypertension but also analyze how these factors affect hypertension and their interrelationships, which is consistent with practical theory better than logistic regression and has a better application prospects.
Sorafenib with concurrent multiple-line therapies improves overall survival in advanced stage hepatocellular carcinoma
Authors: Hsiao Pojen , Hsieh Kun-Chou , Chen Yaw-Sen , Hsu Chia-Chang , Lo Gin-Ho , Li Yu-Chan , Hsieh Pei-Min , Lin Hung-Yu , Wu Tsung-Chin , Yeh Jen-Hao , Lin Chih-Wen 
Published: June 2019

Abstract
The efficacy of sorafenib in combination with transarterial chemoembolization (TACE) or multiple-line therapies in patients with advanced hepatocellular carcinoma (HCC) remains unclear. This study aimed to investigate the overall survival (OS) of patients with advanced HCC in response to different combination therapies.
We analyzed the treatment and OS of 401 patients with Barcelona clinic liver cancer stage C HCC between 2012 and 2017. Mortality was analyzed using multivariate Cox regression, and OS was analyzed by the Kaplan–Meier method.
The mean age was 59 years and males were predominant. During a median follow-up time of 8.6 months (range, 1–80 months), 346 (86.2%) patients died. In the multivariate Cox regression analysis, primary tumor size ≥5 cm, serum alpha-fetoprotein ≥200, and serum albumin ≥3.5 were significantly associated with mortality. In addition, compared with sorafenib alone, multiple-line treatments with sorafenib and multiple-line treatments without sorafenib yielded significantly decreased mortality. In the Kaplan–Meier analysis, sorafenib with TACE, multiple-line treatments with sorafenib, third-line treatments with sorafenib, and multiple-line treatments without sorafenib yielded a significantly better median OS than sorafenib alone.
Sorafenib with concurrent multiple-line therapies significantly improved OS. These combination therapies will provide important information for immunotherapy combination with locoregional therapies in advanced HCC.
Effects of comfort care on symptoms, gastric motility, and mental state of patients with functional dyspepsia
Authors: Xiong Yu , Xing Hui , Hu Lingzhi , Xie Jinzhi , Liu Yuan , Hu Deying 
Published: June 2019

Abstract
Functional dyspepsia (FD) is a common upper gastrointestinal disorder globally, but the current treatments for FD are still unsatisfactory. This study was aimed at investigating the effects of comfort care on symptoms, gastric motility, and mental state of patients with FD.
One hundred consecutive patients with FD treated at the Wuhan Union Hospital (Wuhan, China) between 03/2016 and 02/2018 were randomized to routine nursing and comfort care on the basis of routine nursing (50 patients/group), all for 8 weeks. The primary endpoint was FD symptom score. The secondary endpoint included gastric emptying rate, gastric motility parameters, and depression and anxiety scores. The endpoints were assessed at baseline and after 8 weeks.
There were no significant differences between the 2 groups for gender, age, body mass index, types of FD, and course of disease (P > .05). The symptom score in the comfort care group at week 8 was lower than in the routine nursing group (8.3 ± 2.4 vs 10.2 ± 2.4, P < .001). The gastric emptying rate in the comfort care group at week 8 was higher than in the routine nursing group (28.6 ± 5.1 vs 24.3 ± 5.5, P < .001). The fasting and postprandial dominant frequencies of electrogastrogram in the comfort care group at week 8 were higher than in the routine nursing group (1.9 ± 0.9 vs 1.8 ± 1.0, P = .004; 3.1 ± 1.0 vs 2.1 ± 0.9, P < .001). The postprandial dominant power of electrogastrogram in the comfort care group at week 8 was higher than in the routine nursing group (55.7 ± 11.5 vs 42.3 ± 12.5, P < .001). The cases of abnormal electrogastrogram rhythm in the comfort care group at week 8 were significantly less than in the routine nursing group (P = .003). The self-rating depression scale and self-rating anxiety scale in the comfort care group at week 8 were significantly lower than in the routine nursing group (42.5 ± 6.9 vs 47.3 ± 6.4, P = .001; 41.1 ± 7.2 vs 46.3 ± 6.9, P < .001).
Comfort care reduces the symptoms of patients with FD, increases gastric emptying rate, improves gastric motility, relieves patient's depression and anxiety, and promotes the rehabilitation of the disease.
Proteomics analysis identified serum biomarkers for occupational benzene exposure and chronic benzene poisoning
Authors: Li Peimao , Wu Yuanru , Zhang Zhimin , Lin Dafeng , Wang Dianpeng , Huang Xianqing , Zhang Yanfang 
Published: June 2019

Abstract
The study aimed to find novel effect biomarkers for occupational benzene exposure and chronic benzene poisoning (CBP), which might also provide clues to the mechanism of benzene toxicity.
We performed a comparative serological proteome analysis between healthy control workers with no benzene exposure, workers with short-term benzene exposure, workers with long-term benzene exposure, and CBP patients using 2D-DIGE and MALDI-TOF-MS. Two of the differentially expressed proteins were then selected to be validated by immune turbidimetric analysis.
A total of 10 proteins were found to be significantly altered between different groups. The identified deferentially expressed proteins were classified according to their molecular functions, biological processes, and protein classes. The alteration of 2 important serum proteins among them, apolipoprotein A-I and transthyretin, were further confirmed.
Our findings suggest that the identified differential proteins could be used as biomarkers for occupational benzene exposure and CBP, and they may also help elucidate the mechanisms of benzene toxicity.
Efficacy and safety of endoscopic submucosal dissection for papillary adenocarcinoma-type early gastric cancer
Authors: Park Jae Ho , Kim Ju Seok , Kang Sun Hyung , Moon Hee Seok , Sung Jae Kyu , Jeong Hyun Yong 
Published: June 2019

Abstract
Endoscopic submucosal dissection (ESD) has increasingly been used to treat early gastric cancer (EGC); however, its efficacy in treating papillary adenocarcinoma-type EGC remains unknown.
We sought to identify risk factors for lymph node (LN) metastasis in papillary adenocarcinoma-type EGC and evaluate the clinical outcome after ESD.
This study retrospectively reviewed the medical records of patients who were diagnosed with EGC in our hospital from January 2009 to December 2016. In total, 85 patients had papillary adenocarcinoma-type EGC, of whom 52 and 33 underwent surgical treatment and ESD, respectively. This study analyzed the LN metastasis risk factors and clinical outcomes between these 2 groups and with those of an existing ESD indication group.
LN metastasis occurred in 13 (25.0%) of 52 patients who underwent surgery. Multivariate analysis indicated that lymphovascular invasion was an independent risk factor (odds ratio: 20.624; 95% confidence interval: 19.628–21.497; P = .001). Of 33 patients who underwent ESD, 21 (63.6%) had an absolute indication and 12 (36.4%) had an expanded indication. All 3 (9.1%) patients with non-curative resection underwent additional surgery. The clinical outcomes were compared to those of 926 patients who underwent ESD of non-papillary adenocarcinoma-type EGC. There were no significant differences in curative resection rate (P = .327), procedure-related complication (P = .853), local recurrence (P = 1.000), or overall survival (P = 1.000).
ESD of papillary adenocarcinoma-type EGC showed an acceptable outcome in comparison to an existing ESD indication group. However, these patients exhibit a relatively higher risk of LN metastasis.