bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systemic Inducing Resistance on Cocoa Seedling Against Vascular Streak Dieback By Boron and Silikon Application
Authors: Yuliasmara Fitria , Sukamto Sri , Prawoto A. Adi 
Published: September 2018

Black pod rot disease (BPRD) which is caused by Phytophthora palmivora is one of the main diseases of cocoa cultivations particularly in plantations with wet climate. Black pod rot can develop rapidly under high humidity environments, particularly during rainy seasons. This disease can cause loss of harvest of up to 46.63% in East Java. The various control efforts attempted so far have not resulted in significant improvements. Urea, in addition to functioning as fertilizer, can also produce the ammonia gas which is believed to be able to suppress black pod rot. This research aims to determine the effectiveness of black pod rot control using the combination of lime and urea. This research was conducted from June to September 2013. The materials used in test included sterile soil, black pod rot infected cocoa, urea, and agricultural lime. Observation results showed that ammonia could form from urea. Lime can increase the speed of the formation. The ammonia gas forming from 0.06% urea and 0.3% lime can control the P. palmivora fungus inside the soil. Key words: Pod rot, P. palmivora, urea, lime, ammonia
Growth Inhibition of Colletotrichum gloeosporioides by Trichoderma harzianum, Trichoderma koningii, Bacillus subtilis and Pseudomonas fluorescens
Authors: Nur ‘Aini Febrilia , Sukamto Sri , Wahyuni Dwi , Galuh Suhesti Risma , Ayunin Qurrotun 
Published: September 2018

Colletotrichum gloeosporioides is a disease which can cause significant yield loss of cocoa. The objective of this research is to investigate the abilityof antagonist microbes, Trichoderma harzianum, Trichoderma koningii, Bacillus subtilis and Pseudomonas fluorescens in controlling gloeosporioides biologically in laboratorium condition. The experiment was carried out in Crop Protection Laboratory, Indonesian Coffee and Cocoa Research Institute. Results of this research showed that antagonist fungi, T. harzianum, T. koningii, had a stronger ability in inhibiting growth of C. gloeosporioides about 83% compared to the ability of antagonist bacteria, B. subtilis and P. fluorescens, only about 49%. Key words: Growth inhibition, Colletotrichum gloeosporioides, Trichoderma harzianum, Trichoderma koningii, Bacillus subtilis, Pseudomonas fluorescens.
Pattern of Flushing, Cherelle Wilt, and Accuracy of Yield Forecasting of Some Cocoa Clones
Authors: Prawoto Adi 
Published: September 2018

Monthly observation of cocoa flushing, number of cherelle wilt (CW), number of small, medium and large pods of 6 clones was conducted for two years to study its dynamics for one year. A study was conducted in Kaliwining Experimental Station, 45 m asl. and D rainfall type (according to Schmidt & Ferguson), using ICS 13, ICS 60, TSH 858, Sulawesi 1, Sulawesi 2 and KW 165 clones of 8 years old. Each clone was planted intermittently in separate rows, replicated 6 rows. Correlation and regression analysis were done between variables and with rainfall data. The parallel research was conducted in the similar station to assess the accuracy of production estimation method by identify percentage of small pods (length 1—10 cm), medium (11—15 cm) and large pods (>15 cm) to grow until harvested. The study used 15th years old trees of Sulawesi 1, Sulawesi 2, KW 165, KKM 22, ICS 13 and DR 2 clones. Each clones was replicated 5 times. The result showed that intensive flushing (>50%) occured during January, March, September and November meanwhile no flushing during December and February. Correlation between rainfall and flushing was positive (r=0.27). Effect of clones on flushing frequency was similar but for flushing intensity was significant. KW 165 tended to be the lowest but TSH 858 tend to be the highest. CW occured for a year-round but the height level during May and June. Effect of clones was significant, KW 165 showed highest followed by Sulawesi 2. CW level showed positive correlation with number of medium (r=0.71) and big pods (r=0.55), except showed negative correlation with flushing intensity (r=-0.37) and rainfall (r=-0.51). High pod setting happened during May to November and low pod setting during December to March. In this aspect effect of clones were significant, the productive clones were Sulawesi 1, Sulawesi 2 and KW 165, but ICS 60 was the less. CW level during 1st semester was lower than at 2nd semester and clone effect was significant. The opportunity of small, medium and big pods to be harvested was similar among both semester. The opportunity of small, medium and big pods to be harvested were 8—56%; 57—83% and 77—96% respectively depend on the clones. In average, those opportunities were 27%, 72%, and 87% for small, medium and big pods respectively.Key words: flushing, cherelle wilt, pod setting, clones, yield prediction
Study of Seed Germination by Soaking Methode of Cacao (Theobroma cacao L.)
Authors: Pancaningtyas Sulistyani , Santoso Teguh Iman , . Sudarsianto 
Published: September 2018

Study of germination methods conduct to get information about seed viability based on germination rate, percentage of germination and vigority. Germination methods was studied to get the efficiency and effectivity of germination, easy to handle, low costs with high vigority. Sand and gunny sack methods for germination, need extensive place and 3-4 days germination period after planting. This research will study the alternative of germination method with soaking. This method can be accelerating germination rate and effectively place usage without decreasing the quality of cacao seedling.The research was done at Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institue. This research consist of two experiment was arranged based on factorial completely random design. First experiment will observed to compared germination rate and the second experiment will observed seedling quality between soaking and wet gunny sack germination method.The results showed that length of radicel on soaking method longer than wet gunny sack method. Growth of radicel started from 2 hours after soaking, moreover length of radicel at 4 hours after soaking have significant different value with gunny sack method. On 24 hours after soaking have 3,69 mm and 0,681 mm on wet gunny sack treatment. Except lengt of hipocotyl, there is not different condition between seedling that out came from soaking and wet gunny sack method. Length of hipocotyl on 36 hours after soaking have 9,15 cm and significant different between wet gunny sack germination method that have 5,40 cm. Keywords : seed germination, soaking method, Theobroma cacao L., cocoa seedlings
Ammonia Volatilization in Cocoa Farm as Affected by Sugarcane Filter Cake Supply at Different Urea and Soil Moisture Levels
Authors: Prastowo Erwin , Hikmah Nadya Faiqotul , Baon John Bako 
Published: September 2018

Ammonia (NH3) volatilization is a crucial mechanism in soil nitrogen (N) cycle. It accounts for main loss of N in cocoa farms when environment is suitable to promote the emission. A research to locate a link of several factors, namely, sugarcane filter cake (SFC), urea and soil moisture content to NH 3 volatilization, and to reveal cocoa N uptake and urea fertilizer efficiency was taken place both in a laboratory with the incubation technique, and in a greenhouse as a pot trial. The design was completely randomized design in factorial. The soil was Inceptisol from Kaliwining Experimental Station, Jember, East Java, collected from top soil of a productive cocoa farm. A closed trap system was designed to measurevolatilized NH 3 from soil and SFC mixture incubated for 14 days. For the pot experiment, cocoa clone of Scavina 6 seedlings were grown for six months withtreatments involving series level of SFC and series level of urea. Soil moisture content influenced volatilized NH3 by reducing 50% emission in where it was more obvious in higher level of urea applied. It was more than 90% in average for the increase in volatilized NH 3roduced by increasing in level of urea from 0 to 0.4 g kg -1 in overall level of combinations. An increase in urea level had resulted in increase in soil pH, and Parson correlation suggested a direct link with volatilized NH 3 We found a relationship between SFC and volatilized NH 3 and their combinations with soil moisture and urea content were able to inhibit the emission. Emission decrease up to 28% was observed from 0 to 100 g SFC kg-1. This inhibition may have contributed to the increase in N cocoa plant uptake by more than 70% compared to control and subsequent increase in N fertilizer supply efficiency up to 86% in the combination with 0.2 g urea kg-1. The absence of SFC had made a decline in urea efficiency about 20 times.Keywords: ammonia volatilization, sugarcane filter cake, urea, soil moisture, cocoa
Physiological Character of Cocoa Clones that Cultivated under Three Species of Shade Trees
Authors: Regazzoni Oscar , Sugito Yogi , Suryanto Agus , Prawoto Adi 
Published: September 2018

This experiment aims to know the solar energy efficiency of four clones of cocoa that cultivated under three different shading plants. This experiment has been done from September until December 2013 located at Kaliwining Experiment Farm with characteristic 45 m above sea level, soil type is low humic gley, soil texture is silty clay loam, and climate classification type D based on Scmidht and Fergusson classification. This experiment used nested design as experimental design with species of shading plant as main plot which are teak (Tectona grandis L.), krete (Cassia surattensis (Burm.) F.), lamtoro (Leucaena leucocephala L.) and cocoa clones as sub plot which are Sulawesi 1, Sulawesi 2, KKM 22, and KW 165. The observation of physiological character consists of the level of leaf chlorophyll, the level of leaf nitrogen, spesific leaf area, leaf area index, the amount of stomata, and stomatal density. The experimental result showed that there is differences (heterogenity) between shading location based on homogenity test by Bartlett method. The interaction effect between plant spesies and cocoa clones are affect the leaf area index and stomatal density, whereas the level of leaf chlorophyll, the level of leaf nitrogen, and spesific leaf area was affected by the kind of plant spesies. Keywords : Physiological character, cocoa Ccones, shading trees, nested design, Bartlett method
Application of lime and urea and its effect on development of Phythophthora palmivora.
Authors: Pratama Sakti Widyanta 
Published: September 2018

Black pod rot disease (BPRD) which is caused by Phytophthora palmivora is one of the main diseases of cocoa cultivations particularly in plantations with wet climate. Black pod rot can develop rapidly under high humidity environments, particularly during rainy seasons. This disease can cause loss of harvest of up to 46.63% in East Java. The various control efforts attempted so far have not resulted in significant improvements. Urea, in addition to functioning as fertilizer, can also produce the ammonia gas which is believed to be able to suppress black pod rot. This research aims to determine the effectiveness of black pod rot control using the combination of lime and urea. This research was conducted from June to September 2013. The materials used in test included sterile soil, black pod rot infected cocoa, urea, and agricultural lime. Observation results showed that ammonia could form from urea. Lime can increase the speed of the formation. The ammonia gas forming from 0.06% urea and 0.3% lime can control the P. palmivora fungus inside the soil. Key words: Pod rot, P. palmivora, urea, lime, ammonia
Population of Pratylenchus coffeae (Z.) and growth of Arabica coffee seedling inoculated by Pseudomonas diminuta L. and Bacillus subtilis (C.).
Authors: Fauzi Irfan , Wiryadiputra Soekadar 
Published: September 2018

AbstractPratylenchus coffeae is a parasitic nematoda that infected the roots of some plants, one of them is coffee. The Infection of Pratylenchus coffeae cause root tissue damage that led to root lession and make root become rotten, it will interfere the ability of roots to absorb water and nutrients in the soil which resulted in the growth of plants. At the moment, control of Pratylenchus coffeae are following integrated pests management (IPM) program, which integrated between the use of coffee resistant clone and application of biological agents. Research on biological control was conducted more intensive, at the moment; due to it is friendlier save against environment and cheaper then using chemical nematicides. The research was conducted to know the effects of Micorrhiza Helper Bacteria (MHB),Pseudomonas diminuta and Bacillus subtilis in suppressing the population of P. coffeaeas well as their effect on growth of coffee seedling. Coffee arabica (Coffea arabica L.) seedling one moth old were used in the experiment. The experiment prepared with eight treatments and five replications, as follows: A (Pseudomonas diminuta with density of 108 cfu / ml), B (Pseudomonas diminuta with density of 2x108 cfu / ml), C (Bacillus subtilis with density of 108 cfu / ml ), D (Bacillus subtilis with density 2x108 cfu / ml), E (Carbofuran nematicide 5 g formulation / pot), F (Pseudomonas diminuta and Bacillus subtilis with each density of 108 cfu / ml), K- (Nematoda inoculation but without bacteria and nematicide), K+ (coffee seedling without any additional treatment). The experiment was conducted for sixteen weeks or about four months. The results of the experiment showed that application of MHB could suppress population of P. coffeae and increase coffee seedling growth significantly. Inoculation of B. subtilis at 108 cfu per seedling suppressed significantly nematoda population of 71.3% compared with untreated seedling but inoculated with nematoda. It was not significant different with carbofuran treatment which could suppress nematoda population by 89.7%. The same result also occur on the treatment of P. diminuta at density level 2.108 cfu/seedling, which could suppress nematoda population by 64.2%. Seedling growth were treated with MHB also significantly increase compared with seedling without treatment and inoculation of nematodas, especially on the treatment of B.subtilis at density level 108 cfu and P.diminuta at density level of 108 cfu, with increasing level of 35.4% and 34.2 %, respectively.Keywords: MHB (Mycorrhiza Helper Bacteria), Arabica coffee (Coffea arabica L.), Pratylenchus coffeae, Bacillus subtilis, Pseudomonas diminuta.
Performance of Cooled Cone Grinding Machine in Cocoa Cake Processing
Authors: Firmanto Hendy , Suharyanto Edy , Kuswanto . 
Published: September 2018

The process of cocoa paste pressing has a function to separate the fatty component of cocoa from its cake. Cocoa paste is further processed into cocoa powder using grinding machine for cocoa cake. The cooled cone type of cocoa grinding machine is used to solve the problem of plug in the maschine caused by melting of fat in cocoa cake due to hot effect as a result of friction in the grinding machine. Grinding machine of cocoa has conical form of cylinder for grinding and stator wall wrapped by source of cold and closed with jacket wool. Research was conducted at Kaliwining Experimental Garden of Indonesian Coffee and Cocoa Research Institute (ICCRI) using cocoa cake containing 26.75% originated from Forastero type of cocoa seed. The capacity and recovery of the machine was influenced by space between rotor cylinder and stator wall. Grinding machine operated at cooling temperature of 25.5oC and space between rotor – stator 0.9 cm and the capacity of 187.5 kg/hour with recovery of 200 mesh cocoa powder as much as 24%. The maximum power of machine required was 2.5 kW with efficiency of energy transfer of 97%. Results of proximate analysis showed that there was no change of protein content, but protein and carbohydrate content increased after processing, i.e. from 5.70% and 59.82% into 5.80% and 61.89% respectively.Key words : cocoa cake, cooling, grinding, cocoa powder
Relationship between Physiological Characteristic and Bean Quality on Some Cocoa Clones (Theobroma cacao L.)
Authors: Sari Indah Anita , Zakariyya Fakhrusy , Susilo Agung Wahyu 
Published: September 2018

Photosynthesis is one of the physiological process that influence the bean weight and this process related with the efectiveness of the stomata character and chlorophyll content in the leaves. The research was conducted at Kaliwining Research Station, Indonesian Coffee and Cocoa Research Institute. Design of experiment was randomized complete block design (RCBD) consisted of six clones as treatment were Sulawesi 1, Sulawesi 2, Sca 6, ICS 60, TSH 858, ICCRI 03, PA 300. Each treatment was replicated three times. Stomata resistance diffusion, tranpiration,the content of chlorophyll a, chlorophyll b, chlorophyll total (a+b), bean number and bean weight were observed. The resuts of experiment showed that difference in chlorophyll a, chlorophyll b, chlorophyll total (a+b), stomata resistance diffusion, bean number and bean weight existed within six clones tested. Transpiration rate did not show the significantly different between six clones tested. Sulawesi 1 showed the highest content of chlorophyll a and ICS 60 and ICCRI 03 showed higher content of chlorophyll b than the other clones. Chlorophyll a, b and total (a+b) showed positively influence on bean number and bean weight. Transpiration rate had negatively influence to bean number per pod, on the otherhand it showed positively influence to bean weight. Chlorophyll total (a+b) showed high genetic variance (σg2), high phenotypic variance (σf2) and high estimated value of heritability (H). The chlorophyll a,b had moderate genetic variance, moderate phenotypic variance and high of estimated value of heritability. Chlorophyll total (a+b) could be used a selection criteria based on the value of correlation, genetic variance, phenotypic variance and estimated value of heritability would give high opportunity in selection process.
Apuntando deseos, pasiones y poderes: registrar vínculos para “levantar villa”. Los escribanos de San Felipe de Aconcagua, Chile, 1740-1837
Authors: Albornoz María Eugenia , Argouse Aude 
Published: September 2018

La escritura pública construye vínculos sociales, como ocurre en San Felipe de Aconcagua, entre su fundación (1740) y el orden de los archivos que realiza un escribano en 1836. Más allá de registrar eventos importantes para la ciudad, la actividad de los escribanos, o de quienes aseguran sus registros, muestra que en este periodo la cultura y la práctica jurídicas se transmiten cotidianamente e importan a todos. Igualmente, que los autores y custodios de las escrituras y de los archivos notariales intervienen en el ámbito judicial, político y cultural de la jurisdicción, desde sus trayectorias, afectos y experiencias. Esta humanización de la figura del escribano diluye los estereotipos asociados a su rol y enriquece la comprensión del proceso de “levantar villa”.
Email versus In-Person Audit Inquiry: Recent Research and Additional Survey Data
Authors: Saiewitz Aaron 
Published: September 2018

SUMMARY
Recent research suggests that an auditor's choice to conduct audit inquiry via email versus in person can adversely impact both auditor and client behavior. In this study, I review recent research on this important topic, and I present additional survey-based evidence that describes how clients perceive auditor email versus in-person inquiries. I find that clients generally view auditors' in-person requests as more important and urgent than email requests, and clients feel they can more carefully craft their responses to support their company's preferred reporting position via email compared to in person. Further, clients expect that auditors will question an aggressive response more quickly in person than over email. Although audit partners have expressed concerns that email communication could impede auditor-client relationship building, I find no differences in how much clients like the auditor or in clients' willingness to work again with the auditor after receiving an email or in-person request. However, recent research suggests that email requests can lead to more negative feelings toward the auditor, which could affect the auditor-client relationship over time. I discuss implications for audit firms and provide suggestions for future research.
Effector memory CD4+ T-cells and dendritic cells are noninvasive biomarkers of late cellular rejection after kidney transplantation
Authors: Nosik A. V. , Korotkov S. V. , Smol'nikova V. V. , Grinevich V. Yu. , Efimov D. Yu. , Dmitrieva M. V. , Syantovich A. A. , Dolgolikova A. A. , Kalachik O. V. , Krivenko S. I. , Pikirenya I. I. , Dzyadz'ko A. M. , Scherba A. E. , Rummo O. O. 
Published: September 2018

Introduction. Diagnosis of the kidney transplant cellular rejection in the long-term after transplantation remains a challenge. Usual surrogate markers are not enough sensitive and specific. Rejection is an immune reaction to donor alloantigens. The kidney transplant biopsy to diagnose a dysfunction is an invasive procedure with the incidence of complications about 12.6% and can lead to transplant loss. In this regard, the search of immunological biomarkers for early noninvasive and accurate diagnosis of kidney transplant rejection is an actual task.Material and methods. This is a report of the observational retrospective single-center, comparative case-control study in two groups involving 44 patients who underwent kidney transplantation. The first group (REJ) included the patients with the chronic graft dysfunction caused by a biopsy-confirmed late cellular rejection (22 patients). The second group (STA) included the recipients who had no dysfunction in the posttransplant period (22 patients). Flow cytometry of peripheral blood cells was performed to identify immunophenotyping markers of late cellular rejection after kidney transplantation (we determined subpopulations of T, B lymphocytes, and dendritic cells).Results. As a result of our work, we found significant differences in the absolute count of effector memory T cells making 0.147 (0.115–0.260) × 109 cell/L in REJ group, and 0.106 (0.067–0.136) × 109 cell/L in STA group (р = 0.0167). Relative and absolute counts of myeloid dendritic cells were also different between the groups: 0.65 (0.36–0.73) vs. 1.05 (0.67–1.4) % and 0.039 (0.028–0.056) vs. 0.063 (0.049–0.076) × 109 cell/L, respectively (р = 0.0009, р = 0.003). The numbers of plasmacytoid dendritic cells were also different between the study groups: 0.0038 (0.0021–0.0054) vs. 0.005 (0.0035–0.007) × 109 cell/L for an absolute count (р = 0.0414), and 0.055 (0.04–0.085) vs. 0.09 (0.05–0.12) % for a relative count (р = 0.0197).Conclusion. The obtained data showed that the blood level of dendritic cells, which are the main “professional” initiators of immune reaction, and the level of effector helper T memory cells, which constitute the main lymphocyte subpopulation posing a destructive impact on the kidney transplant, can be considered as diagnostic markers of kidney transplant cellular rejection in the long-term after surgery.