bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating China's Water Security for Food Production: The Role of Rainfall and Irrigation
Authors: Zhu Wenbin , Jia Shaofeng , Devineni Naresh , Lv Aifeng , Lall Upmanu 
Published: September 2019

AbstractChina's water stress and its pressure on food security is a widely recognized crisis. A framework is proposed and applied at the provincial level to evaluate China's water security for food production. It is based on a water stress index that accounts for the deficit between water supply and aggregate demand of 23 major field crops over the study period (1961–2015). The water demand is estimated based on method recommended by the Food and Agriculture Organization of the United Nations. The water supply is composed of rainfall and irrigation. Consequently, the water security of both rainfed and irrigated agriculture is evaluated in our research, and the respective contribution of rainfall and irrigation is distinguished. Suggestions regarding adjustment in irrigation investment and planting area are proposed for practical application. The framework also holds great potential to investigate the impact of climate change and the virtual water flow of crops.
Plant roots increase both decomposition and stable organic matter formation in boreal forest soil
Authors: Adamczyk Bartosz , Sietiö Outi-Maaria , Straková Petra , Prommer Judith , Wild Birgit , Hagner Marleena , Pihlatie Mari , Fritze Hannu , Richter Andreas , Heinonsalo Jussi 
Published: September 2019

AbstractBoreal forests are ecosystems with low nitrogen (N) availability that store globally significant amounts of carbon (C), mainly in plant biomass and soil organic matter (SOM). Although crucial for future climate change predictions, the mechanisms controlling boreal C and N pools are not well understood. Here, using a three-year field experiment, we compare SOM decomposition and stabilization in the presence of roots, with exclusion of roots but presence of fungal hyphae and with exclusion of both roots and fungal hyphae. Roots accelerate SOM decomposition compared to the root exclusion treatments, but also promote a different soil N economy with higher concentrations of organic soil N compared to inorganic soil N accompanied with the build-up of stable SOM-N. In contrast, root exclusion leads to an inorganic soil N economy (i.e., high level of inorganic N) with reduced stable SOM-N build-up. Based on our findings, we provide a framework on how plant roots affect SOM decomposition and stabilization.
Evolution, dynamic expression changes and regulatory characteristics of gene families involved in the glycerophosphate pathway of triglyceride synthesis in chicken (Gallus gallus)
Authors: Yang Liyu , Liu Ziming , Ou Kepeng , Wang Taian , Li Zhuanjian , Tian Yadong , Wang Yanbin , Kang Xiangtao , Li Hong , Liu Xiaojun 
Published: September 2019

AbstractIt is well documented that four gene families, including the glycerophosphate acyltransferases (GPATs), acylglycerophosphate acyltransferases (AGPATs), lipid phosphate phosphohydrolases (LPINs) and diacylglycerol acyltransferases (DGATs), are involved in the glycerophosphate pathway ofde novotriglyceride (TG) biosynthesis in mammals. However, no systematic analysis has been conducted to characterize the gene families in poultry. In this study, the sequences of gene family members in the glycerophosphate pathway were obtained by screening the public databases. The phylogenetic tree, gene structures and conserved motifs of the corresponding proteins were evaluated. Dynamic expression changes of the genes at different developmental stages were analyzed by qRT-PCR. The regulatory characteristics of the genes were analyzed byin vivoexperiments. The results showed that theGPAT,AGPATandLPINgene families have 2, 7 and 2 members, respectively, and they were classified into 2, 4 and 2 cluster respectively based on phylogenetic analysis. All of the genes exceptAGPAT1were extensively expressed in various tissues. Estrogen induction upregulated the expression ofGPAMandAGPAT2, downregulated the expression ofAGPAT3,AGPAT9,LPIN1andLPIN2, and had no effect on the expression of the other genes. These findings provide a valuable resource for further investigation of lipid metabolism in liver of chicken.
Neurobiological and behavioural responses of cleaning mutualisms to ocean warming and acidification
Authors: Paula José Ricardo , Repolho Tiago , Pegado Maria Rita , Thörnqvist Per-Ove , Bispo Regina , Winberg Svante , Munday Philip L. , Rosa Rui 
Published: September 2019

AbstractCleaning interactions are textbook examples of mutualisms. On coral reefs, most fishes engage in cooperative interactions with cleaners fishes, where they benefit from ectoparasite reduction and ultimately stress relief. Furthermore, such interactions elicit beneficial effects on clients’ ecophysiology. However, the potential effects of future ocean warming (OW) and acidification (OA) on these charismatic associations are unknown. Here we show that a 45-day acclimation period to OW (+3 °C) and OA (980 μatm pCO2) decreased interactions between cleaner wrasses (Labroides dimidiatus) and clients (Naso elegans). Cleaners also invested more in the interactions by providing tactile stimulation under OA. Although this form of investment is typically used by cleaners to prolong interactions and reconcile after cheating, interaction time and client jolt rate (a correlate of dishonesty) were not affected by any stressor. In both partners, the dopaminergic (in all brain regions) and serotoninergic (forebrain) systems were significantly altered by these stressors. On the other hand, in cleaners, the interaction with warming ameliorated dopaminergic and serotonergic responses to OA. Dopamine and serotonin correlated positively with motivation to interact and cleaners interaction investment (tactile stimulation). We advocate that such neurobiological changes associated with cleaning behaviour may affect the maintenance of community structures on coral reefs.
Combination treatment with n-3 polyunsaturated fatty acids and ursodeoxycholic acid dissolves cholesterol gallstones in mice
Authors: Jang Sung Ill , Fang Sungsoon , Kim Kwang Pyo , Ko Younhee , Kim Hyoseon , Oh Jieun , Hong Ga Young , Lee Su Yeon , Kim Joon Mee , Noh Ilkoo , Lee Dong Ki 
Published: September 2019

AbstractThe increasing prevalence of cholesterol gallstone disease places an economic burden on the healthcare system. To identify novel therapeutics, we assessed the effects of n-3 polyunsaturated fatty acids (PUFA) in combination with UDCA in a mouse model of cholesterol gallstones. Gallstone dissolution, gallbladder wall thickness, mucin gene expression in the gallbladder, and levels of phospholipids, cholesterol, and bile acids in bile and serum were analysed. RNA was extracted from the liver for mRNA sequencing and gene expression profiling. Combination treatment resulted in greater gallstone dissolution compared with the control group, and PUFA and combination treatments reduced the thickness of the gallbladder wall. Expression levels of mucin genes were significantly lower in the UDCA, PUFA, and combination groups. Transcriptome analyses revealed that combination treatment modulated hepatic lipid metabolism. The PUFA and combination groups showed elevated bile phospholipid and bile acid levels and a lower cholesterol saturation index. Combination treatment with PUFA and UDCA dissolves cholesterol gallstones in mice by decreasing mucin production, increasing levels of phospholipids and bile acids in bile, and decreasing cholesterol saturation. Further studies of the therapeutic effects of combination PUFA and UDCA treatment in patients with cholesterol gallstones are warranted.
Determination and analysis of harmful components in synthetic running tracks from Chinese primary and middle schools
Authors: Wu Xiaoxiao , Li Ning , Ji Hanxu , Zhang Haifeng , Bu Jiangtao , Zhang Xiaoming , Qian Shasha , Yang Yang , Han Bing , Wang Haojie , Ye Ping , Zhou Jungui , Zhang Chi 
Published: September 2019

AbstractIn China, incidences involving pupils suffering health problems caused by synthetic running tracks have attracted the public’s attention. However, the existence of known and unknown harmful chemicals in the tracks have not yet been explored. Here, the levels of 16 known harmful ingredients were firstly analyzed in 167 school running tracks. In all samples, the recognized toxic solvents and additives, such as the benzene series, soluble mercury, 3,3′-dichloro-4,4′-diaminodiphenylmethane (MOCA) and toluene diisocyanate monomer (TDI) were under the limits of detection. In contrast, polycyclic aromatic hydrocarbons (PAHs), phthalates, Short chain chlorinated paraffins (SCCPs) soluble lead, cadmium and chromium were found in 86%, 88%, 46%, 81%, 43% and 83% of the specimens, respectively. The levels, toxicology and distribution of these known chemicals were evaluated. Then, a static-headspace gas chromatography-mass spectrometer (GC-MS) method in full scan mode was employed to screen for unknown volatile chemicals. Three groups of chemicals reflecting different kinds of pollution sources were discovered: new solvents, such as N, N-Dimethylformamide, new additives, such as 2-ethylhexanoic acid, and by-products, such as carbon disulfide. In summary, the existence of potential risk factors in school plastic tracks was revealed through exhaustive testing. Moreover, most of the hazardous components detected have been recently included in a new national standard to improve the safety performance of synthetic running tracks.
Numerical simulations of tunable ultrashort power splitters based on slotted multimode interference couplers
Authors: Huang Chia-Chien , Sun Te-Chia 
Published: September 2019

AbstractThis paper presents an ultracompact tunable device for power splitting and switching by tuning the Fermi energy level of monolayer patternless graphene underneath a slotted multimode interference (MMI) coupler operating in the mid-infrared, λ = 9–11 μm. By introducing a high-index silicon slot in the central region of the MMI structure, which can significantly shorten the beat length, the proposed device has an approximately 4.5-fold reduction in device length and a two-fold improvement in power transmission compared with conventional MMI couplers without slotting. The device has a footprint of only 0.30 × 0.65 μm2 (<λ/10), making it the smallest power splitter and switcher. Over the bandwidth of 2 μm, the power transmission of the proposed device is nearly uniform. Extending the operating bandwidth is limited only by the practically achievable Fermi energy of graphene. For the fabrication tolerance, the numerical results show that the relative power variations are lower than 5%, even though the dimension variations are greater than 15%. With its advantages of tunability, compact footprint, and broadband operation, the proposed device is suitable for highly dense photonic integrated circuits.
The expression of PD-L1 in salivary gland carcinomas
Authors: Vital Domenic , Ikenberg Kristian , Moch Holger , Rössle Matthias , Huber Gerhard F. 
Published: September 2019

AbstractObjective was to analyze the role of PD-L1 and its relation to demographic, patho-clinical and outcome parameters in salivary gland carcinoma (SGC) patients. Patients treated for salivary gland carcinomas between 1994 and 2010 were included. A retrospective chart review for baseline characteristics, pathohistological, clinical and outcome data was performed. Immunohistochemistry for PD-L1 was performed using tissue microarrays. PD-L1 expression was assessed in tumor cells and tumor-infiltrating immune cells (TIIC) and statistical analysis with regard to baseline and outcome data was performed. Expression of PD-L1 (by means ≥1% of the cells with PD-L1 positivity) was present in the salivary gland carcinoma cells of 17%, in the TIIC of 20% and in both tumor cells and TIIC of 10% the patients. PD-L1 expression in tumor cells and both tumor cells and TIIC was related to tumor grading (p = 0.035 and p = 0.031, respectively). A trend towards higher grading was also seen for PD-L1 expression in TIICs (p = 0.058). Patients with salivary duct carcinomas and PD-L1 expressing TIICs showed a significantly worse DFS and OS (p = 0.022 and p = 0.003, respectively), those with both tumor cells and TIIC expressing PD-L1 a significantly worse DFS (p = 0.030). PD-L1 expression is present in 17% and 20% of salivary gland carcinoma cells and TIIC. Ten percent of the patient showed a PD-L1 positivity in both tumor cells and TIIC. This is related to high tumor grading and therefore might be a negative prognostic factor.
REVEL and BayesDel outperform other in silico meta-predictors for clinical variant classification
Authors: Tian Yuan , Pesaran Tina , Chamberlin Adam , Fenwick R. Bryn , Li Shuwei , Gau Chia-Ling , Chao Elizabeth C. , Lu Hsiao-Mei , Black Mary Helen , Qian Dajun 
Published: September 2019

AbstractMany in silico predictors of genetic variant pathogenicity have been previously developed, but there is currently no standard application of these algorithms for variant assessment. Using 4,094 ClinVar-curated missense variants in clinically actionable genes, we evaluated the accuracy and yield of benign and deleterious evidence in 5 in silico meta-predictors, as well as agreement of SIFT and PolyPhen2, and report the derived thresholds for the best performing predictor(s). REVEL and BayesDel outperformed all other meta-predictors (CADD, MetaSVM, Eigen), with higher positive predictive value, comparable negative predictive value, higher yield, and greater overall prediction performance. Agreement of SIFT and PolyPhen2 resulted in slightly higher yield but lower overall prediction performance than REVEL or BayesDel. Our results support the use of gene-level rather than generalized thresholds, when gene-level thresholds can be estimated. Our results also support the use of 2-sided thresholds, which allow for uncertainty, rather than a single, binary cut-point for assigning benign and deleterious evidence. The gene-level 2-sided thresholds we derived for REVEL or BayesDel can be used to assess in silico evidence for missense variants in accordance with current classification guidelines.
Centrifugal Force-Driven Modular Micronozzle System: Generation of Engineered Alginate Microspheres
Authors: Kang Sung-Min , Lee Go-Woon , Huh Yun Suk 
Published: September 2019

AbstractIn this study, we developed a modular micronozzle system that can control the flow of fluid based on centrifugal force and synthesize functional alginate microspheres with various structures and sizes. Our method is to fabricate a programmable microreactor that can be easily manufactured without the conventional soft-lithography process using various sequences of the micronozzles with various inner diameters. To overcome the obstacles of pump-based microfluidic devices that need to be precisely controlled, we designed the programmable microreactor to be driven under centrifugal force with a combination of micronozzles, thus enabling the mass production of various functional alginate microspheres within a few minutes. The programmable microreactor designed through the arrangement of the modular micronozzles enables the formation of various types of alginate microspheres such as core-shell, Janus, and particle mixture. These materials are controlled to a size from 400 µm to 900 µm. In addition, our platform is used to generate pH-responsive smart materials, and to easily control various sizes, shapes, and compositions simultaneously. By evaluating the release process of model drugs according to the pH change, the possibility of drug delivery application is confirmed. We believe that our method can contribute to development of biomaterials engineering that has been limited by the requirement of sophisticated devices, and special skills and/or labor.
Optical and magnetic properties of the ground state of Cr3+ doping ions in REM3(BO3)4 single crystals
Authors: Prokhorov A. A. , Chernush L. F. , Melnik T. N. , Minikayev R. , Mazur A. , Babin V. , Nikl M. , Lančok J. , Prokhorov A. D. 
Published: September 2019

AbstractNew data about the state of Cr3+ doping ions in a single crystal of YGa3(BO3)4 have been obtained by studying different methods. Using electron paramagnetic resonance, it was found that the Cr3+ ions substitute the trivalent gallium ions. The obtained spin Hamiltonian parameters of the Cr3+ ions in the YGa3(BO3)4 single crystal (g = 1.9743 ± 0.0004; D = −0.465 ± 0.001 cm−1; E = −0.013 ± 0.001 cm−1) were analyzed and compared with those in TmAl3(BO3)4, EuAl3(BO3)4, and YAl3(BO3)4. The deviation of the Z-axis in the spectrum from the crystallographic axis С3 is 1,7 degrees in YGa3(BO3)4. In situ X-ray diffraction was used to study the structural and elastic properties of huntite-type borates in the temperature range of RT-1073 K. In the radioluminescence (RL) spectra, the Cr3+-related emission bands were observed in the red wavelength range, and the presence of other defect-related bands was also registered in some cases. Thermally stimulated luminescence (TSL) glow curves were acquired over a wide temperature range, and the trap depths of the most prominent bands were calculated. The 11B NMR spectra show that two nonequivalent spectral components exist for BO3 structural elements.
Non-ideal behavior of a treadmill depends on gait phase, speed, and weight
Authors: Tielke Austin , Ahn Jooeun , Lee Hyunglae 
Published: September 2019

AbstractNoticeable differences exist between treadmill and overground walking; kinematics, kinetics, and muscle activation patterns differ between the two. Many previous studies have attributed the differences to changes in visual information, air resistance, and psychological effects such as fear. In this study, we demonstrate that no treadmill serves as an inertial frame of reference. Considering the linear momentum principle, the finite sampling rate of the controller, and the limited power of the treadmill motor, we predict that 1) the error of the treadmill speed periodically varies depending on the locomotion phase and 2) this non-ideal behavior becomes more evident as the locomotion speed or the weight of the walker increases. Experimental observation confirmed our predictions by quantifying the variation of the actual treadmill belt speed and the ground reaction force in the anterior–posterior direction for different locomotion speeds and subject weights. These results emphasize a need for design criteria like the minimum sampling rate and the minimum motor power that treadmill locomotion studies should consider.
Effect of exogenous spermidine on floral induction, endogenous polyamine and hormone production, and expression of related genes in ‘Fuji’ apple (Malus domestica Borkh.)
Authors: Qin Ling , Zhang Xin , Yan Jie , Fan Lu , Rong Chunxiao , Mo Chuanyuan , Zhang Manrang 
Published: September 2019

AbstractFlower bud formation in ‘Fuji’ apple (Malus domestica Borkh.) is difficult, which severely constrains commercial production. Spermidine (Spd) plays an important role in floral induction, but the mechanism of its action is incompletely understood. To investigate the effect of Spd on flowering, 6-year-old ‘Fuji’ apple trees were treated with 1 × 10−5 mol L−1 Spd to study the responses of polyamines [putrescine (Put), Spd and spermine (Spm)], hormones [gibberellins (GA3) and abscisic acid (ABA)], and polyamine-, hormone- and flowering-related genes. Spd application promoted flowering during floral induction by increasing MdGA2ox2 (gibberellin 2-oxidase) through GA3 reduction and increasing MdNCED1 and MdNCED3 (9-cis-epoxycarotenoid dioxygenase) through ABA enrichment during 60 to 80 days after full bloom. The flowering rate as well as the expressions of flower-related genes, except for MdLEY (LEAFY), also increased, thereby promoting flowering. In addition, spraying with Spd significantly increased the contents of endogenous polyamines except for Spm in terminal buds by increasing the expressions of polyamine-associated genes. We hypothesize that the contribution of Spd to flowering is related to crosstalk among polyamines, hormone signals, and related gene expressions, which suggests that Spd participates in the apple floral induction process.
Urinary estrogens as a non-invasive biomarker of viable pregnancy in the giant panda (Ailuropoda melanoleuca)
Authors: Wilson Kirsten S. , Wauters Jella , Valentine Iain , McNeilly Alan , Girling Simon , Li Rengui , Li Desheng , Zhang Hemin , Rae Mick T. , Howie Forbes , Andrew Ruth , Duncan William Colin 
Published: September 2019

AbstractFemale giant pandas show complex reproductive traits, being seasonally monoestrus, displaying a variable length embryonic diapause and exhibiting pseudopregnancy. Currently, there is no confirmatory non-invasive biomarker of blastocyst implantation or pregnancy. This study aimed to monitor urinary estrogens across gestation in pregnancy (n = 4), pseudopregnancy (n = 4) and non-birth cycles (n = 5) in the giant panda. A pregnancy-specific profile of estrogens corrected for urinary specific gravity was identified during the gestation period. Pregnant females showed increasing concentrations of estrogens for 29 days until birth, no increase was observed during pseudopregnancy and the two profiles were distinguishable from each other for the final 2 weeks of the cycle suggesting the estrogens are of placental origin. This allowed a nomogram, starting at a known fixed point during the cycle, to be created and tested with cycles of known outcome, and cycles which were inseminated but did not result in a birth. Non-birth profiles showed deviations from that of pregnancy. We believe these deviations indicate the point of failure of the placenta to support a developing cub. Non-invasive longitudinal monitoring of estrogen concentrations therefore has the potential to be developed as a panda pregnancy test to predict viable cub development.
Inter-arm Blood Pressure Difference is Associated with Recurrent Stroke in Non-cardioembolic Stroke Patients
Authors: Chang Yoonkyung , Kim Jinkwon , Kim Yong-Jae , Song Tae-Jin 
Published: September 2019

AbstractRecurrent stroke increases mortality and aggravates the disability of stroke patients. We hypothesized that increased inter-arm systolic blood pressure difference and inter-arm diastolic blood pressure difference would be related to recurrent stroke in non-cardioembolic stroke patients. A total of 1226 consecutive non-cardioembolic first-ever ischemic stroke patients, in whom bilateral brachial blood pressures were measured by an automated ankle-brachial index measuring device, were included in our study. Recurrent stroke was defined as newly developed neurologic symptoms with relevant lesions on brain CT and/or MRI after 7 days or hospital discharge. Inter-arm systolic and diastolic blood pressure differences ≥10 mmHg were noted in 9.7% (120/1226) and 5.0% (62/1226) of patients, respectively. During a median 24 months of follow-up, 105 (8.5%) patients experienced recurrent stroke. Patients who had inter-arm systolic blood pressure difference ≥10 mmHg showed increased risk of recurrent stroke (hazard ratio:1.77, 95% confidence interval: 1.04–3.00, p = 0.033). Moreover, inter-arm diastolic blood pressure difference ≥10 mmHg was also independently associated with increased risk of recurrent stroke (hazard ratio:2.92, 95% confidence interval: 1.59–5.34, p = 0.001). In conclusion, inter-arm blood pressure difference ≥10 mmHg may be associated with increased risk recurrent stroke in non-cardioembolic stroke patients.