bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accurate Pan-Cancer Molecular Diagnosis of Microsatellite Instability by Single-Molecule Molecular Inversion Probe Capture and High-Throughput Sequencing
Authors: Waalkes Adam , Smith Nahum , Penewit Kelsi , Hempelmann Jennifer , Konnick Eric Q , Hause Ronald J , Pritchard Colin C , Salipante Stephen J 
Published: April 2018

AbstractBACKGROUNDMicrosatellite instability (MSI) is an emerging actionable phenotype in oncology that informs tumor response to immune checkpoint pathway immunotherapy. However, there remains a need for MSI diagnostics that are low cost, highly accurate, and generalizable across cancer types. We developed a method for targeted high-throughput sequencing of numerous microsatellite loci with pan-cancer informativity for MSI using single-molecule molecular inversion probes (smMIPs).METHODSWe designed a smMIP panel targeting 111 loci highly informative for MSI across cancers. We developed an analytical framework taking advantage of smMIP-mediated error correction to specifically and sensitively detect instability events without the need for typing matched normal material.RESULTSUsing synthetic DNA mixtures, smMIPs were sensitive to at least 1% MSI-positive cells and were highly consistent across replicates. The fraction of identified unstable microsatellites discriminated tumors exhibiting MSI from those lacking MSI with high accuracy across colorectal (100% diagnostic sensitivity and specificity), prostate (100% diagnostic sensitivity and specificity), and endometrial cancers (95.8% diagnostic sensitivity and 100% specificity). MSI-PCR, the current standard-of-care molecular diagnostic for MSI, proved equally robust for colorectal tumors but evidenced multiple false-negative results in prostate (81.8% diagnostic sensitivity and 100% specificity) and endometrial (75.0% diagnostic sensitivity and 100% specificity) tumors.CONCLUSIONSsmMIP capture provides an accurate, diagnostically sensitive, and economical means to diagnose MSI across cancer types without reliance on patient-matched normal material. The assay is readily scalable to large numbers of clinical samples, enables automated and quantitative analysis of microsatellite instability, and is readily standardized across clinical laboratories.
MERAWAT KALI-MERANCANG ASA KOTA KONTESTASI DAN PARTISIPASI KOMUNITAS ATAS DINAMIKA SUNGAI DI KOTA SURAKARTA
Authors: Zunariyah Siti , Ramdhon Akhmad 
Published: April 2018

These cities are change by the development scheme compelled by various economic, politic and cultural interests in central and local relations. In the last decades, the dominant condition of physical aspect-oriented development does not change as well. In some aspects, the scale is getting larger and more massive with decentralization interest. The delegated authority in policy framework gives the local government the foundation of accelerating the change through some autonomous scheme, in which the most dominant orientation among all of those attempts is the city transforming with economic-political reasoning. On the other hand, decentralization also gives the citizens the opportunity of being the part of transformation scheme designed by government. Participation Space allows for the involvement in various policies that will be undertaken in collective contestation in public domain. This article narrates how the urban development scale changes the urban spaces and impacts on the urban ecology, namely river. Narration of river transformation in the last decade is that the river transforms due to many factors and it can be seen from both physical and non-physical transformation. The existing development orientation marginalizes the river and at the same time river assumes the development burden. The crucial point of participation idea is inseparable as well from the relationship between city, river, and citizens staying living in urban kampong daily. The growing response of community is interesting to be studied as the part of understanding the urban physical transformation on the one hand and citizen’s social dynamics on the other hand. Decribing Surakarta and Cities provides the construction of community interest relation complexity and the reinterpretation of river as the part of city. The dynamics of urban kampong in river area can represent the contestation of values, interests and social relations in urban context. Keyword: City, Kampong, River, Community, Participation
CONSUMO DE MEDICAMENTOS POR AGENTES COMUNITÁRIOS DE SAÚDE
Authors: Santos Dominick Danielle Mendonça , Barbosa Thamara Corrêa , Rocha Ricardo Lopes , Bodevan Emerson Cotta , Pimenta Pinheiro Marcos Luciano 
Published: April 2018

Esse trabalho investigou o consumo de medicamentos pelos agentes comunitários de saúde (ACS), e realizou uma capacitação abordando a temática. Participaram do estudo 44 ACS. Os medicamentos mais utilizados foram os analgésicos, antitérmicos e anti-inflamatórios (70,8%). Os ACS relataram a seguinte frequência de uso de medicamentos no último mês: 12 usaram uma vez, seis usaram duas vezes, 17 usaram três vezes ou mais e 18 não usaram medicamentos. Quanto à forma de consumo, 18 realizam a automedicação. Dos entrevistados, 35 não tiveram dificuldade em adquirir medicamentos sob prescrição sem a mesma, seis fazem uso de medicamento sob controle especial e 51 entrevistados utilizam água para tomar o medicamento. Os entrevistados afirmaram não consumirem medicamentos pela influência da propaganda comercial. Diante dos dados obtidos, conclui-se que ocorre a prática da automedicação e falhas no uso racional de medicamentos entre os agentes comunitários de saúde.
ESTUDOS DE EQUIVALÊNCIA FARMACÊUTICA E PERFIL DE DISSOLUÇÃO COMPARATIVO DE MEDICAMENTOS CONTENDO HIDROCLOROTIAZIDA
Authors: Ribeiro Cássio Carvalho , Andrade Giovana Maria Marques , Couto Renê Oliveira 
Published: April 2018

A hidroclorotiazida (HCTZ) é um fármaco da classe dos diuréticos tiazídicos, amplamente utilizado no tratamento de pacientes com edema e hipertensão arterial sistêmica leve a moderada, sendo considerado altamente efetivo pelos profissionais da saúde. O objetivo deste trabalho foi avaliar a equivalência farmacêutica e realizar estudos comparativos de perfil de dissolução entre medicamento Referência (R) e três medicamentos Genéricos (G1, G2 e G3) na forma de comprimidos de liberação imediata de HCTZ (25 mg), de acordo com a monografia apresentada na Farmacopeia Brasileira 5ª Ed. (2010) e a legislação nacional vigente. Nos testes físicos e físico-químicos todos os medicamentos G foram aprovados, no entanto, em alguns casos apresentaram diferenças significativas quando comparados ao medicamento R. Em relação ao perfil de dissolução, o qual visa avaliar o fator de semelhança F2, apenas o medicamento G3 não apresentou um perfil semelhante quando comparado ao medicamento R. A intercambialidade entre G3 e R pode estar comprometida, pois há possibilidade de não atingir níveis plasmáticos da HCTZ na velocidade e extensão desejadas. Os resultados deste trabalho podem refletir a qualidade e confiabilidade dos medicamentos genéricos amplamente utilizados pela população brasileira.
Postharvest control of anthracnose lesions and its causative agent, Colletotrichum musae by some oils
Authors: Rizwana Humaira 
Published: April 2018

Anthracnose of banana is incited by Colletotrichum musae. It is recognized as one the most destructive diseases of mature and immature banana fruits, resulting in huge economic losses all over the world. Present research deals with screening some oils both in vitro and in vivo for their antifungal activity against C.musae. Clove oil (0.1µl/ml) completely arrested the conidial germination and mycelial growth of C. musae. Fenugreek and almond oil exhibited significant inhibition of mycelial growth, 61% and 57% at a concentration of 2µl/ml. However, olive oil was least inhibitory on the test fungi. Clove oil also a showed marked reduction in anthracnose lesions on banana fruits, thereby suggesting disease control. Scanning electron microscopy revealed severely damaged mycelium and conidia. FTIR studies show the presence of important bands representing phenols, terpenes, aldehydes, and ketones. Based on our findings; clove, fenugreek and almond oil demonstrated fungicidal and fungistatic activities against anthracnose pathogen. Hence, these oils can be considered as potential alternatives to chemical treatments.
Evidence Integration in Natural Acoustic Textures during Active and Passive Listening
Authors: Górska Urszula , Rupp Andre , Boubenec Yves , Celikel Tansu , Englitz Bernhard 
Published: April 2018

AbstractMany natural sounds can be well described on a statistical level, for example, wind, rain, or applause. Even though the spectro-temporal profile of these acoustic textures is highly dynamic, changes in their statistics are indicative of relevant changes in the environment. Here, we investigated the neural representation of change detection in natural textures in humans, and specifically addressed whether active task engagement is required for the neural representation of this change in statistics. Subjects listened to natural textures whose spectro-temporal statistics were modified at variable times by a variable amount. Subjects were instructed to either report the detection of changes (active) or to passively listen to the stimuli. A subset of passive subjects had performed the active task before (passive-aware vs passive-naive). Psychophysically, longer exposure to pre-change statistics was correlated with faster reaction times and better discrimination performance. EEG recordings revealed that the build-up rate and size of parieto-occipital (PO) potentials reflected change size and change time. Reduced effects were observed in the passive conditions. While P2 responses were comparable across conditions, slope and height of PO potentials scaled with task involvement. Neural source localization identified a parietal source as the main contributor of change-specific potentials, in addition to more limited contributions from auditory and frontal sources. In summary, the detection of statistical changes in natural acoustic textures is predominantly reflected in parietal locations both on the skull and source level. The scaling in magnitude across different levels of task involvement suggests a context-dependent degree of evidence integration.
Sex Differences in Recombination in Sticklebacks
Authors: Sardell Jason M , Cheng Changde , Dagilis Andrius J , Ishikawa Asano , Kitano Jun , Peichel Catherine L , Kirkpatrick Mark 
Published: April 2018

AbstractRecombination often differs markedly between males and females. Here we present the first analysis of sex-specific recombination in Gasterosteus sticklebacks. Using whole-genome sequencing of 15 crosses between G. aculeatus and G. nipponicus, we localized 698 crossovers with a median resolution of 2.3 kb. We also used a bioinformatic approach to infer historical sex-averaged recombination patterns for both species. Recombination is greater in females than males on all chromosomes, and overall map length is 1.64 times longer in females. The locations of crossovers differ strikingly between sexes. Crossovers cluster toward chromosome ends in males, but are distributed more evenly across chromosomes in females. Suppression of recombination near the centromeres in males causes crossovers to cluster at the ends of long arms in acrocentric chromosomes, and greatly reduces crossing over on short arms. The effect of centromeres on recombination is much weaker in females. Genomic differentiation between G. aculeatus and G. nipponicus is strongly correlated with recombination rate, and patterns of differentiation along chromosomes are strongly influenced by male-specific telomere and centromere effects. We found no evidence for fine-scale correlations between recombination and local gene content in either sex. We discuss hypotheses for the origin of sexual dimorphism in recombination and its consequences for sexually antagonistic selection and sex chromosome evolution.
A adequabilidade do controle interno no Comando da Aeronáutica: uma percepção endógena
Authors: Menezes Montenegro Tânia , Alves Morais de Oliveira Lídia Cristina , De Souza Lopes Marcello 
Published: April 2018

As nações desenvolvidas têm promovido o avanço de metodologias de gestão que otimizem o desempenho da Administração Pública, cujos alicerces são a direção, o controle e a accountability das organizações. Neste contexto, e tendo por base a concepção de instituições internacionais das áreas da Administração, Contabilidade e Auditoria, o objetivo deste artigo é analisar a adequabilidade dos Sistemas de Controle Interno Organizacional (SCIO) em funcionamento nas Unidades Gestoras (UG) do Comando da Aeronáutica (COMAER) do Brasil, valendo-se das percepções dos seus agentes responsáveis. Constatou-se que OS SCIO do COMAER revelam uma aderência moderada às boas práticas internacionais de controle interno, embora se conclua que, nalgumas áreas, essa aderência permaneça ainda num estágio inicial.
Seguridad del paciente en las prácticas formativas de un programa de pregrado en Terapia Ocupacional
Authors: Peñas Felizzola Olga Luz , Parra-Esquivel Eliana I. , Gómez-Galindo Ana M. 
Published: April 2018

Objetivo Identificar condiciones y experiencias sobre la seguridad del paciente en prácticas académicas de terapia ocupacional de la Universidad Nacional de Colombia.Métodos Estudio descriptivo transversal, con una muestra integrada por 29 estudiantes de práctica académica del programa Terapia Ocupacional. Diseño y aplicación de una encuesta sobre atención segura de usuarios (versión electrónica), sometida a revisión de expertos para validar su contenido. Previamente, cada estudiante dio su consentimiento para la realización de la encuesta.Resultados Las caídas de los pacientes son las que se escapan más frecuentemente del reporte o registro en el sitio de práctica (47,1 % de los estudiantes nunca las reporta). A pesar de esto, es el evento que mayor registro presenta con respecto a otros reportes (41,1 %). En menor proporción los estudiantes siempre dejan reporte de los incidentes relacionados con documentación y registro, y con equipos y dispositivos de atención (21,5 % y 28,6 %, respectivamente). En estas dos últimas categorías son más numerosos los estudiantes que sólo algunas veces reportan lo ocurrido (46,4 % en ambos casos).Discusión Es necesario avanzar en la indagación sobre las causas y consecuencias de los eventos que afectan la seguridad del paciente en las prácticas de los estudiantes, y en la disposición de medidas de mejora que sean eficientes junto con el impacto que tienen al implementarse. Se requieren esfuerzos para transformar la posible percepción punitiva que pueda estar recayendo sobre el seguimiento de las medidas para la seguridad del paciente.Todo lo anterior podría estar explicando parte del considerable subregistro de incidentes que documenta la literatura y que coincide con los hallazgos de este estudio.
The effect of ethanol extract of myrmecodia pendens to tgf-β1 expression and osteoblast cells after tooth extraction (the experimental research on cavia cobaya)
Authors: Ismardianita Efa , Nasrul Ellyza , Yanwirasti Yanwirasti , Hemiawati Mieke 
Published: April 2018

Objective: Myrmecodia pendens is often used as a traditional medicine to treat various disease. A previous study, ethanol extract of sarang semut plant (Hypnophytum furmicarum jack) 4.65 mg was effective in fastening establishment of socket granulation tissue after tooth extraction, the Myrmecodia pendens 3% too fasten healing soft tissue of wound after tooth extraction. The aim of this study to determine the effect of ethanol extract of Myrmecodia pendens to the expression of TGF-β1, and the number of osteoblasts after tooth extraction.Material and Methods: this study using 24 male marmoots were divided into 2 groups. The entire groups had extract done 1st left incisor mandibula, into the socket control group applied 0,1 ml of 0.5% CMC and treatment group was given 0,1 ml of ethanol extract Myrmecodia pendens 10% dissolved with CMC 0.5%. On day 3,7,14 and 21, marmoots were decapitated and histopathologic preparations then observed the expression of TGF-β1, and the number of osteoblast cell. Data analyzed by Kruskall-wallis test with probability p<0.05 and Mann-Whitney to determine differences in effectiveness between variables.Results: Based on the Kruskal-wallis test, there is a significant difference the TGF-β1 expression and the number of osteoblast cell which had giving ethanol extract of Myrmecodia pendens between of control group and group treatment (p<0.05), further test with Mann-Whitney for TGF-β1 with days of observation shown a significant difference meanwhile osteoblast with days of observation showed no significant difference.Conclusion: the ethanol extract Myrmecodia pendans can assist the wound healing process after tooth extraction by increasing the expression of TGF-β1, and osteoblast cells number
The effect of submersion denture base acrylic resin in a betel leaf ekstract solution against growth candida albicans
Authors: Pawinru Ardiansyah S. , Izham Andi 
Published: April 2018

Objective: Microorganisms are often found in the oral cavity is candida albicans approximately 40 % in the oral cavity. Candida albicans can penetrate the acrylic resin that can infect the soft tissue and is the cause of denture stomatitis therefore the disinfection pf denture base is an important factor that must be done. The purpose of the research is to determine how the effect of submersion denture base acrylic resin in a betel leaf exstract solution against growth candida albicans.Material and Methods: This research is an experimental laboratory with a longitudinal design (follow-up) study. The Sampling method used is Total Sampling. This study used a sample of 12 pieces of denture base material acrylic resin to be suspended candida albicans fungus for 1x24 hours at 37oC.Results: The number of candida albicans colonies n denture base acrylic resin which soaked betel leaf extract solution that the dilution 10-1with consentration 2.5% total colony count is 2 and the results 2.0 x 101 CFU/ml , on a control solution that the dilution 10-2 total colony 355 and the result 3.55x104 CFU/ml , that the dilution 10-3 total colony 62 and the result 6.2x104 CFU/ml.Conclusion: Betel leaf extract (piper linn) can inhibit the growth of candida albicans on denture base of acrylic resin, betel leaf extract (piper linn) 5% is a minimal anti-fungal power that can be used as a disinfectant solution in acrylic resin denture.
The use of bioabsorbable poly-L-lactide acid miniplate and screw (FIXSORB®-MX ) for bone fixation in mandibular fracture: cases reports
Authors: Tsuji Tsuakasa , Kitada Hideaki , Abe Sinnosuke 
Published: April 2018

Objective: This report aims to elucidate the degree of degradation of bioabsorbable poly-L-lactide acid (PLLA) devices and the influence of these materials on the surrounding tissues in the human body.Methods: Two patients with fractured mandibles underwent treatment using a biodegradable fixation system. In the first case, the plates and screws were removed along with extraction of an impacted wisdom tooth at 6 months after surgery, and in the second case, the same procedure was performed 12 months after surgery. Changes in the physical properties of the materials and histologic alterations in the periosteum and bone associated with the bioabsorbable plates were assessed.Results: The materials demonstrated adequate degradation in the human body, and skeletal stability proved sufficient for the duration of mandibular bone healing without complications after surgery.Conclusion: On the basis these findings, it can be concluded that PLLA implants are useful for the fixation of human mandibular fractures.
Parosteal osteosarcoma: a diagnostic dilemma on non-healing following tooth extractions: a case report
Authors: Damanhuri Rahma Y. , Prihartiningsih Prihartiningsih , Rahajoe Poerwati S. 
Published: April 2018

Objective: This paper reports a case of mandibular ramus parosteal osteosarcoma, discussing diagnostic problems and reviewing treatment variations. Methods: A 37 year old male patient was referred by a private hospital with a history of abscesses treatment and removal of his right lower molar 1.5 years ago. The wound in his tooth socket was not healed. Swelling with abscesses recurred and was diagnosed as osteomyelitis. The Orthopantomogram (OPG) result showed a description of osteomyelitis, and the Multi Slice Computed Tomography (MSCT) 3D confirmed the suspected osteosarcoma. Histopathologic results indicated parosteal osteosarcoma. Result: Hemimandibulectomy and chemotherapy were performed with good results.Conclusion: This parosteal osteosarcoma was difficult to diagnosis quickly because the initial image resembled osteomyelitis but it was low grade and rare; therefore, meticulous and accurate diagnosis was deemed necessary. Chemotherapy-combined surgery was thereby performed for treating this parosteal osteosarcoma.
THE DIFFERENCE BETWEEN DEVELOPING SINGLE PAGE APPLICATION AND TRADITIONAL WEB APPLICATION BASED ON MECHATRONICS ROBOT LABORATORY ONAFT APPLICATION
Authors: Solovei V. , Olshevska O. , Bortsova Y. 
Published: April 2018

Today most of desktop and mobile applications have analogues in the form of web-based applications. With evolution of development technologies and web technologies web application increased in functionality to desktop applications. The Web application consists of two parts of the client part and the server part. The client part is responsible for providing the user with visual information through the browser. The server part is responsible for processing and storing data.MPA appeared simultaneously with the Internet. Multiple-page applications work in a "traditional" way. Every change eg. display the data or submit data back to the server. With the advent of AJAX, MPA learned to load not the whole page, but only a part of it, which eventually led to the appearance of the SPA. SPA is the principle of development when only one page is transferred to the client part, and the content is downloaded only to a certain part of the page, without rebooting it, which allows to speed up the application and simplify the user experience of using the application to the level of desktop applications.Based on the SPA, the Mechatronics Robot Laboratory ONAFT application was designed to automate the management process. The application implements the client-server architecture. The server part consists of a RESTful API, which allows you to get unified access to the application functionality, and a database for storing information. Since the client part is a spa, this allows you to reduce the load on the connection to the server and improve the user experience