bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism insights and experimental feasibility of using boron nitride nanocones for rapid adsorption and degradation of SF6 decomposition compounds
Authors: Hadizadeh Mohammad Hassan , Hu Yongxia , Xu Fei , Wang Wenxing 
Published: November 2024

AbstractGas-insulated switchgear (GIS) employs sulfur hexafluoride (SF6) as an insulating medium to shield electrical gadget. However, SF6 can decompose under sure situations, generating dangerous sulfur-based totally compounds which include SO2, SOF2, and SO2F2. These byproducts pose enormous dangers to both protection and environmental integrity. Efficiently adsorbing and disposing of those compounds is critical for ensuring operational reliability and reducing environmental dangers. This study investigates the adsorption and degradation mechanisms of SF₆ decomposition compounds (SO₂, SOF₂, and SO₂F₂) on boron nitride nanocones (BNNCs) using density functional theory (DFT) and ab initio molecular dynamics (AIMD) simulations. Our comprehensive analysis covers five distinct systems, exploring individual and combined adsorption scenarios. The findings reveal that the apex of BNNCs plays a crucial role in the adsorption process, showing high efficiency in adsorbing SO₂ (adsorption energy − 1.22 eV) and facilitating the catalytic breakdown of SOF₂ (adsorption energy − 1.57 eV). The positively charged potential at the nanocone’s apex significantly influences the dissociation and subsequent adsorption of fluorine atoms, with an energy barrier for F dissociation at the apex (1.8 kcal/mol) much lower than at the sidewall (5.3 kcal/mol). In gas mixtures, SO₂ preferentially binds to the apex region of BNNCs, with a bond length of approximately 1.38 Å. BNNCs demonstrate superior adsorption capabilities for SO₂ and SOF₂ compared to other boron nitride nanostructures, with adsorption energies up to 89% higher. The electron transfer analysis reveals that BNNC complexes act as potent electron donors, particularly in the case of BNNC@3SO₂F₂. Additionally, BNNCs show significant potential as sensors for detecting SO₂F₂, with a rapid recovery time of 4.67 ps and a notable decrease in the Fermi level energy to -4.97 eV upon adsorption. The study also provides insights into the angular distribution and charge density difference profiles, offering a detailed understanding of the adsorption mechanisms. These findings have important implications for improving the safety and efficiency of gas-insulated switchgear (GIS) and contribute to the development of more effective environmental protection solutions in electrical power systems.
A survey of beliefs, attitudes, knowledge, and behaviors about evidence-based practice in physical therapists of Latin America: a cross-sectional study
Authors: Morales-Osorio Marco Antonio , Ordoñez-Mora Leidy T. , Gutiérrez-Espinoza Héctor , Araya-Quintanilla Felipe , Bays-Moneo Ana , Ramírez-Vélez Robinson 
Published: November 2024

AbstractEvidence-based practice (EBP) refers to the integration of scientific evidence into the clinical setting. This study aimed to determine whether Latin American physiotherapists use EBP, analyze the associated factors, barriers, and facilitators, and examine the relationships between these elements in clinical practice. To conduct this cross-sectional study, the web-based questionnaire survey method was chosen. Survey was self-administered questionnaires to assess understanding of various EBP-related terms and determine their knowledge and ability to apply these concepts in clinical practice. The association between attitudes, awareness, and knowledge scores and the demographic data such as age group, years of experience, professional membership registry, and self-reported education was assessed. The questionnaire was distributed to a total of 5000 physical therapists, of which 4099 (82%) responded from seven Latin American countries (Argentina, Bolivia, Chile, Colombia, México, Perú, and Venezuela), between June 1, 2020, and May 31, 2024. Overall, the physical therapists had positive attitudes, beliefs, and interests in EBP. Their educational background, knowledge, and skills related to assessing and interpreting information were associated to the use of EBP. In addition, 53% of respondents indicated that lack of time was the main obstacle to the use of EBP. The top 3 barriers included insufficient time (53%), lack of information resources (20.8%), and lack of research tools (13.6%). Among Latin American physical therapists, individual characteristics workplace, clinical experience, educational background, and demographic characteristics, may behave as facilitators or barriers when performing an EBP. Thus, identifying methods and strategies to support physical therapists in adopting EBP in the Latin American is necessary. The academization of physiotherapy training might change this in the future.
Protein phosphatase-1 regulates the binding of filamin C to FILIP1 in cultured skeletal muscle cells under mechanical stress
Authors: Kokot Thomas , Zimmermann Johannes P. , Schwäble Anja N. , Reimann Lena , Herr Anna L. , Höfflin Nico , Köhn Maja , Warscheid Bettina 
Published: November 2024

AbstractThe actin-binding protein filamin c (FLNc) is a key mediator in the response of skeletal muscle cells to mechanical stress. In addition to its function as a structural scaffold, FLNc acts as a signaling adaptor which is phosphorylated at S2234 in its mechanosensitive domain 20 (d20) through AKT. Here, we discovered a strong dephosphorylation of FLNc-pS2234 in cultured skeletal myotubes under acute mechanical stress, despite high AKT activity. We found that all three protein phosphatase 1 (PP1) isoforms are part of the FLNc d18-21 interactome. Enzymatic assays demonstrate that PP1 efficiently dephosphorylates FLNc-pS2234 and in vitro and in cells upon PP1 activation using specific modulators. FLNc-pS2234 dephosphorylation promotes the interaction with FILIP1, a mediator for filamin degradation. Altogether, we present a model in which dephosphorylation of FLNc d20 by the dominant action of PP1c prevails over AKT activity to promote the binding of the filamin degradation-inducing factor FILIP1 during acute mechanical stress.
AEBP1 is a negative regulator of skeletal muscle cell differentiation in oral squamous cell carcinoma
Authors: Okazaki Fumika , Yorozu Akira , Sekiguchi Shohei , Niinuma Takeshi , Maruyama Reo , Kitajima Hiroshi , Yamamoto Eiichiro , Ishiguro Kazuya , Toyota Mutsumi , Hatanaka Yui , Nishiyama Koyo , Ogi Kazuhiro , Kai Masahiro , Takano Kenichi , Ichimiya Shingo , Miyazaki Akihiro , Suzuki Hiromu 
Published: November 2024

AbstractThe tumor microenvironment plays a pivotal role in cancer development. We recently reported that in oral squamous cell carcinoma (OSCC), adipocyte enhancer-binding protein 1 (AEBP1) is abundantly expressed in cancer-associated fibroblasts (CAFs), leading to CAF activation and inhibition of CD8 + T cell infiltration. In the present study, we investigated whether AEBP1 contributes to the destruction and atrophy of muscle tissues in OSCC. By analyzing human skeletal muscle myoblasts (HSMMs), we found that AEBP1 is downregulated during muscle cell differentiation. Transcriptome analysis revealed that AEBP1 knockdown significantly upregulates myogenesis-related genes in HSMMs, and qRT-PCR and western blot analyses confirmed the induction of muscle-related genes, including MYOG, in HSMMs after AEBP1 knockdown. Conversely, ectopic expression of AEBP1 strongly suppressed myogenesis-related genes in HSMMs. Notably, indirect co-culture of HSMMs with OSCC cells led to AEBP1 upregulation and robust suppression of muscle-related genes in HSMMs. Treatment with TGF-β1 also upregulated AEBP1 and suppressed expression of muscle-related genes in HSMMs. Our findings suggest that AEBP1 is a negative regulator of skeletal muscle cell differentiation and that OSCC cells inhibit muscle cell differentiation, at least in part, by inducing AEBP1.
Exploring the ceRNA network involving AGAP2-AS1 as a novel biomarker for preeclampsia
Authors: Lu Fan , Zeng Ni , Xiao Xiang , Wang Xingxing , Gong Han , Lei Houkang 
Published: November 2024

AbstractPreeclampsia (PE) is an important research subject in obstetrics. Nevertheless, the underlying mechanisms of PE remain elusive. PE-related expression datasets (GSE96983, GSE96984 and GSE24129) were downloaded from the Gene Expression Omnibus (GEO) database. Firstly, the differentially expressed messenger RNAs (DE-mRNAs), DE-microRNA (DE-miRNAs) and DE-long non-coding RNA (DE-lncRNAs) between PE and control cohorts were identified, and the ceRNA network was constructed. Then candidate hub genes were obtained through five algorithms by the protein-protein intersection (PPI) network of the mRNAs. Further, five hub genes were identified by receiver operating characteristic (ROC) curve and gene expression profiles: DAXX, EFNB1, NCOR2, RBBP4 and SOCS1. The function of 5 hub genes was analyzed and the interaction between drugs and hub genes was predicted. A total of 5 small molecule drugs were predicted, namely benzbromarone, 9,10-phenanthrenequinone, chembl312032, insulin and aldesleukin. AGAP2-AS1 was mainly located in exosome and cytoplasm. Agap2-as1-related regulatory subnetworks were extracted from ceRNA networks which included 41 mRNAs, 2 miRNAs and 1 lncRNA, including the regulated relationship pairs AGAP2-AS1-hsa-miR-497-5p-SRPRB, and AGAP2-AS1-hsa-miR-195-5p-RPL36. In summary, we constructed a competitive endogenous RNA (ceRNA) network to identify five potential biomarkers (DAXX, EFNB1, NCOR2, SOCS1 and RBBP4) of PE. The in-depth analysis of the AGAP2-AS1 regulatory network will help to uncover more important molecules closely related to PE and provide a scientific Reference.
Artificial intelligence assisted operative anatomy recognition in endoscopic pituitary surgery
Authors: Khan Danyal Z. , Valetopoulou Alexandra , Das Adrito , Hanrahan John G. , Williams Simon C. , Bano Sophia , Borg Anouk , Dorward Neil L. , Barbarisi Santiago , Culshaw Lucy , Kerr Karen , Luengo Imanol , Stoyanov Danail , Marcus Hani J. 
Published: November 2024

AbstractPituitary tumours are surrounded by critical neurovascular structures and identification of these intra-operatively can be challenging. We have previously developed an AI model capable of sellar anatomy segmentation. This study aims to apply this model, and explore the impact of AI-assistance on clinician anatomy recognition. Participants were tasked with labelling the sella on six images, initially without assistance, then augmented by AI. Mean DICE scores and the proportion of annotations encompassing the centroid of the sella were calculated. Six medical students, six junior trainees, six intermediate trainees and six experts were recruited. There was an overall improvement in sella recognition from a DICE of score 70.7% without AI assistance to 77.5% with AI assistance (+6.7; p < 0.001). Medical students used and benefitted from AI assistance the most, improving from a DICE score of 66.2% to 78.9% (+12.8; p = 0.02). This technology has the potential to augment surgical education and eventually be used as an intra-operative decision support tool.
Mevalonate pathway inhibition reduces bladder cancer metastasis by modulating RhoB protein stability and integrin β1 localization
Authors: Wang Gang , Peng Tianchen , Chen Liang , Xiong Kangping , Ju Lingao , Qian Kaiyu , Zhang Yi , Xiao Yu , Wang Xinghuan 
Published: November 2024

AbstractThe progression and outcome of bladder cancer (BLCA) are critically affected by the propensity of tumor metastasis. Our previous study revealed that activation of the mevalonate (MVA) pathway promoted migration of BLCA cells; however, the exact mechanism is unclear. Here we show that elevated expression of MVA pathway enzymes in BLCA cells, correlating with poorer patient prognosis by analyzing single-cell and bulk-transcriptomic datasets. Inhibition of the MVA pathway, either through knockdown of farnesyl diphosphate synthase (FDPS) or using inhibitors such as zoledronic acid or simvastatin, led to a marked reduction in BLCA cell migration. Notably, this effect was reversed by administering geranylgeranyl pyrophosphate (GGPP), not farnesyl pyrophosphate (FPP) or cholesterol, indicating the specificity of geranylgeranylation for cell motility. Moreover, we found that RhoB, a Rho GTPase family member, was identified as a key effector of the impact of the MVA pathway on BLCA metastasis. The post-translational modification of RhoB by GGPP-mediated geranylgeranylation influenced its protein stability through the ubiquitin-proteasome pathway. Additionally, overexpression of RhoB was found to block the membrane translocation of integrin β1 in BLCA cells. In summary, our findings underscore the role of the MVA pathway in BLCA metastasis, providing insights into potential therapeutic targets of this malignancy.
Greening of India and revival of the South Asian summer monsoon in a warmer world
Authors: Clément Charlotte , Martinez Philippe , Yin Qiuzhen , Clemens Steven , Thirumalai Kaustubh , Prasad Srinivasan , Anupama Krishnamurthy , Su Qianqian , Lyu Anqi , Grémare Antoine , Desprat Stéphanie 
Published: November 2024

AbstractWhile it is accepted that the tropical hydrological cycle has intensified during past interglacial periods due to changes in insolation, greenhouse gases and ice volume, their respective influences are uncertain. Here we present a pollen record from Bengal Bay to reconstruct vegetation changes in India’s core monsoon zone during two warm periods, the current and last interglacial, comparing the data with numerical model simulations to assess the influence of different forcing mechanisms. Results show tropical forest expansion between 11.7-5 ka and 127-120 ka, defining two Indian humid periods, with the last interglacial showing the strongest monsoon activity, consistent with salinity reconstructions. Model-data comparison highlights boreal summer insolation as the primary driver of vegetation dynamics and monsoon intensity during interglacial periods, with CO2 and ice-sheets having a limited effect. Vegetation remains unaffected by pre-industrial CO2 variations above 250 ppmv, a threshold value that characterizes most interglacials of the last million years.
JAK-STAT1 as therapeutic target for EGFR deficiency-associated inflammation and scarring alopecia
Authors: Strobl Karoline , Klufa Jörg , Jin Regina , Artner-Gent Lena , Krauß Dana , Novoszel Philipp , Strobl Johanna , Stary Georg , Vujic Igor , Griss Johannes , Holcmann Martin , Farlik Matthias , Homey Bernhard , Sibilia Maria , Bauer Thomas 
Published: November 2024

AbstractThe hair follicle stem cell niche is an immune-privileged microenvironment, characterized by reduced antigen presentation, thus shielding against permanent immune-mediated tissue damage. In this study, we demonstrated the protective role of hair follicle-specific epidermal growth factor receptor (EGFR) against scarring hair follicle destruction. Mechanistically, disruption of EGFR signaling generated a cell-intrinsic hypersensitivity within the JAK-STAT1 pathway, which, synergistically with interferon gamma expressing CD8 T-cell and NK-cell-mediated inflammation, compromised the stem cell niche. Hair follicle-specific genetic depletion of either JAK1/2 or STAT1 or therapeutic inhibition of JAK1/2 ameliorated the inflammation, restored skin barrier function and activated the residual stem cells to resume hair growth in mouse models of epidermal and hair follicle-specific EGFR deletion. Skin biopsies from EGFR inhibitor-treated and cicatricial alopecia patients revealed an active JAK-STAT1 signaling signature along with upregulation of antigen presentation and downregulation of key components of the EGFR pathway. Our findings offer molecular insights and highlight a mechanism-based therapeutic strategy for addressing chronic folliculitis associated with EGFR-inhibitor anti-cancer therapy and cicatricial alopecia.
Exhaled Breath Volatile Organic Compounds in Pregnancy: A Pilot Study
Authors: Farghaly Mohsen A.A. , Abuelazm Somaya , Farrell Ruth M. , Elgendy Marwa M. , Grove David , Abu-shaweesh Jalal M. , Dweik Raed A. , Aly Hany 
Published: November 2024

Objective This study aimed to assess the volatile organic compounds (VOC)s in breath samples collected noninvasively from pregnant women during pregnancy and postpartum and compare it with nonpregnant controls.
Study Design This pilot study included 50 subjects: 10 pregnant patients in their first trimester, 10 in second trimester, 10 in third trimester, 10 in the first postpartum week, and 10 nonpregnant subjects as a control. We collected exhaled breath from subjects who reported to be healthy and free of any respiratory symptoms. Clinical and demographic data were collected. The samples were analyzed for VOCs using a selected-ion flow-tube mass spectrometer.
Results The VOCs monitored were 22 compounds selected for their common presence in exhaled breath. During pregnancy and postpartum period, there were differences in five compounds mainly: 2-propanol, acrylonitrile, 1-nonene, 2-nonene, and hydrogen sulfide. Significant differences in VOCs were identified during each trimester compared with controls.
Conclusion VOCs could be measured safely and noninvasively in pregnant women. VOCs differed significantly among nonpregnant, pregnant women, and postpartum period. The utilization of this novel assay to identify fetal conditions or identifying women at risk of premature delivery, which should be further investigated in future studies.
Key Points
The PPP - Perineal Body Preserving PSARP (Posterior Sagittal Anorectoplasty) for Anorectal Malformation with Rectovestibular Fistula in Females—Report of Early Outcomes
Authors: Xu Thomas O. , Ryan Julia Ann , Feng Christina , Badillo Andrea , Sandler Anthony , Levitt Marc A. 
Published: November 2024

Abstract
Introduction The perineal body preserving posterior sagittal anorectoplasty (PSARP) (PPP) is a novel modification of the original PSARP for female patients with rectovestibular fistulas designed to eliminate the risk of perineal body dehiscence. This study aims to examine the outcomes following PPP.
Methods A retrospective, single-institution study was performed examining female patients with rectovestibular fistula who underwent PPP between January /2020 and December 2023. Exposure was done through the intended anoplasty only. Perineal body or posterior sagittal incision was not utilized. No routine postoperative dilations were performed. The postoperative outcomes, day of discharge, time to first feeding, and early stooling patterns were assessed.
Results A total of 15 patients underwent a PPP at a median age of 6 months (range 2 days to 19 months) with median follow-up of 11 months (range 1–36). Three (20%) patients underwent repair within the first 3 months of life. Five (33%) had diverting ostomies prior to referral to our team. There was no incidence of dehiscence or rectal prolapse. Two (13%) patients developed an anal stricture which required revision. Fourteen (93%) patients resumed normal feeds on postoperative day 1. Eleven (73%) were discharged on postoperative day 1. All patients were stooling spontaneously at their most recent clinical encounter with 11 (73%) utilizing laxatives.
Conclusions PPP eliminates the risk of perineal body dehiscence and has a quick return to regular diet and home. There is a 13% stricture rate which could relate to a difference in the distal rectal mobilization compared with the traditional PSARP.
Facile Synthesis of 2- and 3-Substituted Indoles Using a Catalytic Amount of Pd/C and 1-Octene System
Authors: Hayashi Masahiko , Dong Xu , Matsubara Ryosuke 
Published: November 2024

AbstractA novel and efficient synthesis of 3- and 2-substituted indoles starting from 2-(2-nitro-1-phenylethyl or 2-nitro-1-alkyl)cyclohexanones and 1,4-diketones, respectively, using a catalytic amount of Pd/C and 1-octene system is reported.
Neoteric Solvents for Metal-Catalyzed Coupling Reactions
Authors: Langellotti Vincenzo , Melchiorre Massimo , Ruffo Francesco , Cucciolito Maria Elena , Esposito Roberto 
Published: November 2024

AbstractCatalysis is a green methodology aimed at optimizing synthetic procedures by simplifying the design of target molecules and reducing energy and material consumption. However, catalytic reactions often rely on polar aprotic solvents, such as dimethylformamide (DMF) or acetonitrile, which present environmental and health issues. In response, manufacturers and researchers are exploring greener alternatives derived from residual biomass, which reduce the negative environmental impact of traditional solvents. These new classes of solvents are termed ‘neoteric’ in order to distinguish them from traditional solvents with well-established applications. This graphical review highlights key findings on the use of these new solvents in metal-catalyzed coupling reactions.
Resveratrol-Loaded Dissolving Microneedles: Fabrication and in vitro Evaluation
Authors: Xing Banghuai , Luo Qian , Gan Tiantian , Jin Yi , Feng Jianfang , Cheng Meng , Tu Liangxing 
Published: November 2024

AbstractResveratrol (Res), an active ingredient derived from a multitude of plants, exhibits multiple pharmacological activities. However, its poor water solubility and low bioavailability present significant challenges to its clinical application. Our study aimed to improve the transdermal absorption of Res using dissolving microneedle (MN) technology, which could effectively overcome the stratum corneum barrier. Res-loaded dissolving microneedles (Res-MNs) were fabricated using polyvinyl pyrrolidone K90 (PVP K90) as the matrix material, and a two-step casting procedure was employed. The process was optimized using the Box–Behnken experimental design approach. The characteristics of Res-MNs in vitro, including morphology, solubility, safety evaluation, and skin permeation, were studied. The results showed that the optimum preparation conditions of Res-MNs were a centrifugation time of 10 minutes, a solvent concentration of 25%, and a prescription ratio (Res: matrix) of 0.375. The skin permeability of the Res-MNs was enhanced compared with Res suspension and Res gel. The cumulative release of Res-MNs in vitro was 75%, which was approximately 5 and 3 times that of the Res suspension group and Res gel group. These results suggest that dissolving MNs may represent a potential approach for enhancing the transdermal delivery of poorly absorbed drugs such as Res.
Arthroscopic Management of Metacarpophalangeal Joint Synovial Chondromatosis
Authors: Mekki Yosra Magdi , Sandon Alexander , Hagert Elisabet 
Published: November 2024

AbstractSynovial chondromatosis is a rare condition where cartilaginous nodules form within the synovium, which can detach and cause loose bodies within the joint. While it typically affects larger joints, involvement of the metacarpophalangeal (MCP) joints is rarely described. We report the case of a 42-year-old female patient who had a slow-growing mass in her right third MCP (MCP III) joint, associated with limited finger motion, swelling, and pain. The diagnosis was confirmed using imaging studies. The patient underwent arthroscopic excision of multiple tumors and synovectomy via dorsal radial and ulnar MCP III portals. Postoperatively, there was rapid improvement in hand function, including normal and pain-free range of motion. This case highlights the benefits of arthroscopic management for synovial chondromatosis in the MCP joint, as it offers advantages like minimal invasiveness, reduced tissue damage, faster recovery times, and lower infection risk compared with traditional open methods.