bluuebunny/crossref_metadata_2025_split huggingface dataset:
The N-fixing legume Periandra mediterranea constrains the invasion of an exotic grass (Melinis minutiflora P. Beauv) by altering soil N cycling
Authors: Nogueira Carina B. , Menéndez Esther , Ramírez-Bahena Martha Helena , Velázquez Encarna , Peix Álvaro , Mateos Pedro F. , Scotti Maria Rita 
Published: July 2019

AbstractMelinis minutiflora is an invasive species that threatens the biodiversity of the endemic vegetation of the campo rupestre biome in Brazil, displacing the native vegetation and favouring fire spread. As M. minutiflora invasion has been associated with a high nitrogen (N) demand, we assessed changes in N cycle under four treatments: two treatments with contrasting invasion levels (above and below 50%) and two un-invaded control treatments with native vegetation, in the presence or absence of the leguminous species Periandra mediterranea. This latter species was considered to be the main N source in this site due to its ability to fix N2 in association with Bradyrhizobia species. Soil proteolytic activity was high in treatments with P. mediterranea and in those severely invaded, but not in the first steps of invasion. While ammonium was the N-chemical species dominant in plots with native species, including P.mediterranea, soil nitrate prevailed only in fully invaded plots due to the stimulation of the nitrifying bacterial (AOB) and archaeal (AOA) populations carrying the amoA gene. However, in the presence of P. mediterranea, either in the beginning of the invasion or in uninvaded plots, we observed an inhibition of the nitrifying microbial populations and nitrate formation, suggesting that this is a biotic resistance strategy elicited by P. mediterranea to compete with M. minutiflora. Therefore, the inhibition of proteolytic activity and the nitrification process were the strategies elicited by P.mediterranea to constrain M.munitiflora invasion.
Inverse Design Tool for Ion Optical Devices using the Adjoint Variable Method
Authors: Neustock Lars Thorben , Hansen Paul C. , Russell Zachary E. , Hesselink Lambertus 
Published: July 2019

AbstractWe present a computer-aided design tool for ion optical devices using the adjoint variable method. Numerical methods have been essential for the development of ion optical devices such as electron microscopes and mass spectrometers. Yet, the detailed computational analysis and optimization of ion optical devices is still onerous, since the governing equations of charged particle optics cannot be solved in closed form. Here, we show how to employ the adjoint variable method on the finite-element method and Störmer-Verlet method for electrostatic charged particle devices. This method allows for a full sensitivity analysis of ion optical devices, providing a quantitative measure of the effects of design parameters to device performance, at near constant computational cost with respect to the number of parameters. To demonstrate this, we perform such a sensitivity analysis for different freeform N-element Einzel lens systems including designs with over 13,000 parameters. We further show the optimization of the spot size of such lenses using a gradient-based method in combination with the adjoint variable method. The computational efficiency of the method facilitates the optimization of shapes and applied voltages of all surfaces of the device.
Amino acids as nutritional factors and (p)ppGpp as an alarmone of the stringent response regulate natural transformation in Micrococcus luteus
Authors: Lichev Antoni , Angelov Angel , Cucurull Inigo , Liebl Wolfgang 
Published: July 2019

AbstractNatural competence for genetic transformation refers to the natural ability of various bacteria to take up exogenous DNA from their surroundings and to incorporate internalized genetic information into their genomes. By promoting bacterial diversification and adaptability, this process represents a major driving force in bacterial evolution. Micrococcus luteus was one of the first organisms used to study natural transformation in bacteria. Since then, however, only very little information about this phenomenon has been reported in M. luteus or in any member of the Actinobacteria phylum (low-GC Gram-positive bacteria). Previous work in our group indicated major differences between the transformation apparatus of M. luteus and the transformation machinery described for various Gram-negative and Gram-positive model bacteria belonging to the phyla Proteobacteria and Firmicutes (high-GC Gram-positive bacteria). This prompted us to initiate a study concerning the regulation mechanism of competence development in M. luteus. In this report, we identify amino acids as a nutritional factor that influences competence in a concentration-dependent manner. By using a transcriptional reporter strain for one of the late competence genes, we demonstrate how increasing concentrations of both amino acids mixtures and single amino acids supplemented to the growth medium affect transformability on transcriptional and post-transcriptional level. Furthermore, we revisit previously generated auxotrophic mutants to show that the transformation machinery is turned down during a state of extreme hunger for amino acids presumably as a part of a general response to auxotrophy. Finally, by generating and analysing knockout mutants for two predicted stringent response enzymes, we provide evidence for the involvement of the alarmone (p)ppGpp as a putative mediator of the effects on transformation development caused by amino acids. As a member of the Actinobacteria phylum, M. luteus could serve as a model for other representatives of the phylum, including a number of important human pathogens.
An Exact Approach to Elimination of Leakage in a Qubit Embedded in a Three-level System
Authors: Sun Yifan , Zhang Jun-Yi , Wu Lian-Ao 
Published: July 2019

AbstractLeakage errors damage a qubit by coupling it to other levels. Over the years, several theoretical approaches to dealing with such errors have been developed based on perturbation arguments. Here we propose a different strategy: we use a sequence of finite rotation gates to exactly eliminate leakage errors. The strategy is illustrated by the recently proposed charge quadrupole qubit in a triple quantum dot, where there are two logical states to encode the qubit and one leakage state. We found an su(2) subalgebra in the three-level system, and by using the subalgebra we show that ideal Pauli x and z rotations, which are universal for single-qubit gates, can be generated by two or three propagators. In addition, the magnitude of detuning fluctuation can be estimated based on the exact solution.
Nano-hydroxyapatite in oral care cosmetics: characterization and cytotoxicity assessment
Authors: Coelho Catarina C. , Grenho Liliana , Gomes Pedro S. , Quadros Paulo A. , Fernandes Maria H. 
Published: July 2019

AbstractNano-hydroxyapatite has been used as an oral care ingredient, being incorporated in several products for the treatment of dental hypersensitivity and enamel remineralisation. Despite its promising results, regulatory and safety concerns have been discussed and questioned by the European Scientific Committee on Consumer Safety (SCCS) regarding the usage of hydroxyapatite nanoparticles in oral care products. In this work, a commercially available nano-hydroxyapatite was characterized and its cytocompatibility towards human gingival fibroblasts was evaluated, as well as its irritation potential using the in vitro HET-CAM assay. All the conditions chosen in this study tried to simulate the tooth brushing procedure and the hydroxyapatite nanoparticles levels normally incorporated in oral care products. The commercial hydroxyapatite nanoparticles used in this study exhibited a rod-like morphology and the expected chemical and phase composition. The set of in vitro cytotoxicity parameters accessed showed that these nanoparticles are highly cytocompatible towards human gingival fibroblasts. Additionally, these nanoparticles did not possess any irritation potential on HET-CAM assay. This study clarifies the issues raised by SCCS and it concludes that this specific nano-hydroxyapatite is cytocompatible, as these nanoparticles did not alter the normal behaviour of the cells. Therefore, they are safe to be used in oral care products.
Artificial Light at Night Promotes Activity Throughout the Night in Nesting Common Swifts (Apus apus)
Authors: Amichai Eran , Kronfeld-Schor Noga 
Published: July 2019

AbstractThe use of artificial light at night (ALAN) is a rapidly expanding anthropogenic effect that transforms nightscapes throughout the world, causing light pollution that affects ecosystems in a myriad of ways. One of these is changing or shifting activity rhythms, largely synchronized by light cues. We used acoustic loggers to record and quantify activity patterns during the night of a diurnal bird – the common swift – in a nesting colony exposed to extremely intensive artificial illumination throughout the night at Jerusalem’s Western Wall. We compared that to activity patterns at three other colonies exposed to none, medium, or medium-high ALAN. We found that in the lower-intensity ALAN colonies swifts ceased activity around sunset, later the more intense the lighting. At the Western Wall, however, swifts remained active throughout the night. This may have important implications for the birds’ physiology, breeding cycle, and fitness, and may have cascading effects on their ecosystems.
Carbon nanotubes-based PdM bimetallic catalysts through N4-system for efficient ethanol oxidation and hydrogen evolution reaction
Authors: Begum Halima , Ahmed Mohammad Shamsuddin , Lee Dong-Weon , Kim Young-Bae 
Published: July 2019

AbstractTransitional metal-nitrogen-carbon system is a promising candidate to replace the Pt-based electrocatalyst due to its superior activity, durability and cost effectiveness. In this study, we have designed a simple strategy to fabricate carbon nanotubes-supported binary-nitrogen-carbon catalyst via wet-chemical method. Palladium and transitional metals (M, i.e. manganese cobalt and copper) nanoparticles are anchored through four-nitrogen system onto carbon nanotubes (denoted as PdM-N4/CNTs). This material has been used as bifunctional electrocatalyst for electrochemical ethanol oxidation reaction and hydrogen evolution reaction for the first time. The N4-linked nanoparticles onto carbon nanotubes plays a crucial role in intrinsic catalytic activity for both reactions in 1 M KOH electrolyte. Among three PdM-N4/CNTs catalysts, the PdMn-N4/CNTs catalyst exhibits higher catalytic activity in terms of current density, mass activity and stability compared to the benchmark Pt/C. The robust electrocatalysis are inherited from the better attachment of PdMn through N4-system onto carbon nanotubes, comparatively smaller particles formation with better dispersion and higher electrical conductivity.
Neural effects of hand-grip-activity induced fatigue sensation on appetite: a magnetoencephalography study
Authors: Matsuo Takashi , Ishii Akira , Nakamura Chika , Ishida Rika , Yamaguchi Takahiro , Takada Katsuko , Uji Masato , Yoshikawa Takahiro 
Published: July 2019

AbstractIt has been reported that physical activity not only increases energy expenditure, but also affects appetite. However, little remains known about the effects of physical activity-induced fatigue sensation on appetite. In the present study, classical conditioning related to fatigue sensation was used to dissociate fatigue sensation from physical activity. The participants were 20 healthy male volunteers. After overnight fasting, on day 1, the participants performed hand-grip task trials for 10 min with listening to a sound. The next day, they viewed food images with (target task) and without (control task) listening to the sound identical to that used on day 1, and their neural activity during the tasks were recorded using magnetoencephalography. The subjective levels of appetite and fatigue sensation were assessed using a visual analog scale. The subjective level of fatigue increased and that of appetite for fatty foods showed a tendency toward increase in the target task while the subjective level of fatigue and that of appetite for fatty foods were not altered in the control task. In the target task, the decrease of theta (4–8 Hz) band power in the supplementary motor area (SMA), which was observed in the control task, was suppressed, and the suppression was positively correlated with appetite for fatty foods, suggesting hand grip activity-induced fatigue sensation may increase the appetite for fatty food; this increase could be related to neural activity in the SMA. These findings are expected to contribute to the understanding of the neural mechanisms of appetite in relation to fatigue.
Interpreting and integrating big data in non-coding RNA research
Authors: Cantarella Simona , Di Nisio Elena , Carnevali Davide , Dieci Giorgio , Montanini Barbara 
Published: July 2019

Abstract
In the last two decades, we have witnessed an impressive crescendo of non-coding RNA studies, due to both the development of high-throughput RNA-sequencing strategies and an ever-increasing awareness of the involvement of newly discovered ncRNA classes in complex regulatory networks. Together with excitement for the possibility to explore previously unknown layers of gene regulation, these advancements led to the realization of the need for shared criteria of data collection and analysis and for novel integrative perspectives and tools aimed at making biological sense of very large bodies of molecular information. In the last few years, efforts to respond to this need have been devoted mainly to the regulatory interactions involving ncRNAs as direct or indirect regulators of protein-coding mRNAs. Such efforts resulted in the development of new computational tools, allowing the exploitation of the information spread in numerous different ncRNA data sets to interpret transcriptome changes under physiological and pathological cell responses. While experimental validation remains essential to identify key RNA regulatory interactions, the integration of ncRNA big data, in combination with systematic literature mining, is proving to be invaluable in identifying potential new players, biomarkers and therapeutic targets in cancer and other diseases.
Machine learning in APOGEE
Authors: Garcia-Dias Rafael , Allende Prieto Carlos , Sánchez Almeida Jorge , Alonso Palicio Pedro 
Published: July 2019

Context. The vast volume of data generated by modern astronomical surveys offers test beds for the application of machine-learning. In these exploratory applications, it is important to evaluate potential existing tools and determine those that are optimal for extracting scientific knowledge from the available observations.
Aims. We explore the possibility of using unsupervised clustering algorithms to separate stellar populations with distinct chemical patterns.
Methods. Star clusters are likely the most chemically homogeneous populations in the Galaxy, and therefore any practical approach to identifying distinct stellar populations should at least be able to separate clusters from each other. We have applied eight clustering algorithms combined with four dimensionality reduction strategies to automatically distinguish stellar clusters using chemical abundances of 13 elements. Our test-bed sample includes 18 stellar clusters with a total of 453 stars.
Results. We have applied statistical tests showing that some pairs of clusters (e.g., NGC 2458–NGC 2420) are indistinguishable from each other when chemical abundances from the Apache Point Galactic Evolution Experiment (APOGEE) are used. However, for most clusters we are able to automatically assign membership with metric scores similar to previous works. The confusion level of the automatically selected clusters is consistent with statistical tests that demonstrate the impossibility of perfectly distinguishing all the clusters from each other. These statistical tests and confusion levels establish a limit for the prospect of blindly identifying stars born in the same cluster based solely on chemical abundances.
Conclusion. We find that some of the algorithms we explored are capable of blindly identify stellar populations with similar ages and chemical distributions in the APOGEE data. Even though we are not able to fully separate the clusters from each other, the main confusion arises from clusters with similar ages. Because some stellar clusters are chemically indistinguishable, our study supports the notion of extending weak chemical tagging that involves families of clusters instead of individual clusters.
Aus der Spur
Authors: Blumenrode Stefan 
Published: July 2019

ZusammenfassungEin Delir kann vielfältige Ursachen und Symptome haben. Wird es zu spät
diagnostiziert, können die Folgen für die Betroffenen lebensbedrohlich sein. Wie
Sie ein Delir erkennen und welche Behandlungsansätze es gibt, erläutert unser
Autor.
Beziehungsarbeit in der Psychiatrie
Authors: Bauer Rüdiger 
Published: July 2019

ZusammenfassungDer Begriff der Beziehungsarbeit wird in der psychiatrischen Pflege oft bemüht,
dabei aber häufig der Beliebigkeit überlassen: Jeder versteht darunter etwas
anderes. Unser Autor beschreibt – basierend auf einer
anthropologisch/evolutionären, naturwissenschaftlich/neurowissenschaftlichen und
humanistisch/zuwendenden Sichtweise – einen neuen theoretischen Rahmen für eine
professionelle pflegerische Beziehungsarbeit in der Psychiatrie. Außerdem
skizziert er Instrumente, die in der Praxis bereits vielfach angewendet
werden.
Association among Clopidogrel Cessation, Platelet Function, and Bleeding in Coronary Bypass Surgery: An Observational Trial
Authors: Petricevic Mate , Knezevic Jadranka , Biocina Bojan , Mikus Mislav , Konosic Lucija , Rasic Mario , Milosevic Milan , Rotim Cecilija , Madzar Tomislav , Rotim Ante , Gasparovic Hrvoje , Goerlinger Klaus 
Published: July 2019

Objectives This study sought to determine (1) the association between the length of preoperative clopidogrel discontinuation, blood loss, and transfusion requirements and (2) whether preoperative platelet function testing predicts excessive postoperative bleeding in patients undergoing coronary artery bypass grafting (CABG) surgery.
Methods In this retrospective analysis, patients undergoing CABG were divided into three groups with regard to the period between preoperative clopidogrel cessation and surgery: group 1 (n = 94, ≤3 days), group 2 (n = 100, 4–5 days), and group 3 (n = 83, 6–7 days), respectively. Impedance aggregometry (Multiplate) with arachidonic acid (ASPI) test assay (used for platelets stimulation) and adenosine diphosphate (ADP) test (used for platelets stimulation) was performed before the surgery. Primary outcome was 24 hours chest tube output (CTO) and transfusion requirements (red blood cell concentrate [RBCC], platelet concentrate [PC], fibrinogen concentrate [FC], and fresh-frozen plasma [FFP]) were considered as secondary outcomes.
Results CTO during 24 hours was significantly higher in group 1 as compared with groups 2 and 3, respectively (p = 0.003). Considering secondary outcomes, RBCC (p = 0.043), PC (p = 0.001), FC (p = 0.003), and FFP (p = 0.010) were more frequently transfused in group 1 as compared with groups 2 and 3, respectively. Multiple electrode aggregometry ASPI and ADP tests were significantly correlated with the 24-hour CTO (ASPI test—rho = −0.258, p < 0.001; ADP test—rho = −0.164, p = 0.007). A significant correlation was observed between clopidogrel-free interval and 24-hour CTO (rho = −0.200, p < 0.001). Receiver-operating characteristics (ROC) curve analysis revealed cutoff values to delineate bleeding tendency (ASPI test ≤ 25 area under the aggregation curve [AUC], ADP test ≤63 AUC, and clopidogrel-free interval ≤3 days).
Conclusion Excessive postoperative bleeding occurred less frequently if the period between clopidogrel discontinuation and surgery was longer than 3 days, as compared with shorter waiting time. Inadequate recovery of the platelets function following clopidogrel cessation in preoperative period was associated with increased bleeding risk. Platelet function testing was found to be a useful tool for postoperative bleeding management in our hands.
Unilateral Pedicled Pectoralis Major Harvested by Endoscopic-Assisted Method Achieves Adequate Management of Sternal Infection and Mediastinitis
Authors: Lin Chih-Hung , Lin Cheng-Hung , Tsai Feng-Chun , Lin Pyng-Jing 
Published: July 2019

Background Bilateral PM muscles or combination with rectus abdominis or omentum are commonly used for upper and lower sternal wound infections. Unilateral PM harvesting using endoscopic-assisted method may have a simple, safe, and reliable entire muscle harvesting with comparable result of less donor-site violation.
Methods A retrospective review was performed from 2003 till 2015 on 38 patients referred to a single plastic surgeon for treatment of sternal wound infection following median sternotomy for cardiovascular surgery. After the humerus insertion of PM was cut with the assistance of endoscope visualization, all the other PM insertions on the sternum, rib, and clavicle were divided, the unilateral pedicled PM can be advanced approximately 10 cm to cover the cephalad and caudal sternum, and fill the retrosternal mediastinum.
Results Four re-explorations in three patients for postoperative hematoma occurred. No early recurrent infection for wound dehiscence experienced. Three patients died of multiple organs failures as 30-day mortality. Two patients underwent late recurrent infections; one patient had twice wire infection removals at 4 and 6 months after transfer, and the other had another PM for rib osteomyelitis in 3 years.
Conclusion Unilateral PM transfer is justified to provide a simple, reliable, straightforward procedure for sternal infection management and mediastinal obliteration without violation of second flap in compromised patients.
Subtle differentiation of countries’ responsibilities under the Paris Agreement
Authors: Pauw Pieter , Mbeva Kennedy , van Asselt Harro 
Published: July 2019

AbstractTo be effective and secure participation, a global climate change agreement needs to be perceived as fair by the countries involved in it. The Paris Agreement approached differentiation of countries’ responsibilities to address climate change by departing from the rigid distinction between industrialised and developing countries through the inclusion of ‘subtle differentiation’ of specific subsets of countries (e.g., Least Developed Countries) for certain substantive issues (e.g., climate finance) and/or for specific procedures (e.g., timelines and reporting). In this article, we analyse whether the self-differentiation countries followed when formulating their own climate plans, or nationally determined contributions (NDCs), is consistent with the Paris Agreement’s subtle differentiation. We find that there is consistency for mitigation and adaptation, but not for support (climate finance, technology transfer and capacity building). As NDCs are the main instrument for achieving the Paris Agreement’s long-term goals, this inconsistency needs to be addressed to allow subsequent rounds of NDCs to be more ambitious.
Infants expect leaders to right wrongs
Authors: Stavans Maayan , Baillargeon Renée 
Published: July 2019

Significance
Social scientists have proposed that expectations about leadership-based power asymmetries have gradually evolved over the course of human history. Some of these expectations concern leaders’ responsibilities to their followers. In particular, leaders are expected to intervene in within-group transgressions and confront the wrongdoers. In violation-of-expectation experiments, we examined whether 17-mo-old infants would share this expectation about leaders. When a fairness transgression occurred among a group of bear puppets, infants expected a leader to intervene and rectify this transgression, but they held no particular expectation for intervention from a nonleader. Thus, consistent with claims that abstract expectations about leaders’ responsibilities are part of the human endowment, our findings indicate that such expectations are already present by the second year of life.