bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of American Joint Committee on Cancer Eighth Edition clinical stage and smoking history on oncologic outcomes in human papillomavirus‐associated oropharyngeal squamous cell carcinoma
Authors: Hawkins Peter G. , Mierzwa Michelle L. , Bellile Emily , Jackson William C. , Malloy Kelly M. , Chinn Steven B. , Spector Matthew E. , Shuman Andrew G. , Stucken Chaz L. , McLean Scott A. , Bradford Carol R. , Prince Mark E. , Carey Thomas E. , Worden Francis P. , Swiecicki Paul L. , Taylor Jeremy M. G. , Wolf Gregory T. , Eisbruch Avraham , Casper Keith A. 
Published: February 2019

AbstractBackgroundThe purpose of this study was to evaluate the AJCC eighth edition clinical staging system for human papillomavirus (HPV)‐associated oropharyngeal squamous cell carcinoma and to further understand how clinical stage and smoking history affect oncologic outcomes. The purpose of this study was to present the understanding of how clinical stage and smoking history affect oncologic outcomes in human papillomavirus (HPV)‐associated oropharyngeal squamous cell carcinoma (SCC) is critical for selecting patients for treatment deintensification.MethodsKaplan‐Meier and Cox regression were used to evaluate overall survival (OS), locoregional recurrence‐free survival (LRFS), and distant recurrence‐free survival (DRFS). Concordance statistics (C‐indices) were used to compare discriminating ability.ResultsThe OS and DRFS but not LRFS were significantly distributed using the American Joint Committee on Cancer (AJCC) seventh and eighth editions criteria. The C‐indices for OS, LRFS, and DRFS were 0.57, 0.54, and 0.60, respectively, using the AJCC seventh edition, and 0.63, 0.53, and 0.65, respectively, using the AJCC eighth edition. On multivariate analysis, 1 + pack‐year smoking history correlated with OS (hazard ratio [HR] 1.96; 95% confidence interval [CI] 1.2‐3.1; P < .01) but not LRFS or DRFS.ConclusionThese results support implementation of the AJCC eighth edition for HPV‐associated oropharyngeal SCC. Clinical stage may be more important than smoking history in selection for deintensification.
Transoral supraglottic laryngectomy using a next‐generation single‐port robotic surgical system
Authors: Orosco Ryan K. , Tam Kenric , Nakayama Meijin , Holsinger F. Christopher , Spriano Giuseppe 
Published: February 2019

AbstractBackgroundTo investigate the feasibility of transoral robotic surgery (TORS) supraglottic laryngectomy (SGL) using a next‐generation flexible surgical robot.MethodsPreclinical human cadaver anatomic study of TORS SGL via en bloc resection.ResultsA single‐port robotic surgical system (da Vinci Sp, Intuitive Surgical, Inc., Sunnyvale, California) provided sufficient access, reach, and visualization to perform TORS SGL. Access and exposure were achieved with a standard laryngo‐pharyngoscope retractor. The remote center of the robotic system was located 10 cm from the maxillary alveolus. Three surgical instruments and one flexible camera could be deployed with minimal collision or restriction of arm movement.ConclusionsRoutine resection supraglottic cancers through TORS have been hindered by challenging exposure and visualization and limited instrument maneuverability deep within the laryngopharyngeal complex. This preclinical feasibility study demonstrates the technical feasibility for TORS SGL using a next‐generation flexible surgical robot.
Predictors of in‐hospital mortality after ischemic stroke: A prospective, single‐center study
Authors: Kortazar‐Zubizarreta Izaro , Pinedo‐Brochado Ana , Azkune‐Calle Itxaso , Aguirre‐Larracoechea Urko , Gomez‐Beldarrain Marian , Garcia‐Monco Juan Carlos 
Published: February 2019

AbstractBackground and AimsStroke is the second leading cause of death around the globe. Studies examining the predictors of in‐hospital mortality and the impact of complications on early outcome of acute ischemic stroke are scant. The aim of this study was to identify predictors of in‐hospital mortality in patients with acute ischemic stroke.MethodsThis was a prospective, single‐center study of patients with acute ischemic stroke consecutively admitted to the Neurology Department of a general hospital during a 2‐year period (from January 1, 2010 to December 31, 2011). Prospective data from this single‐center study included variables related to sociodemographics, comorbidities, and medical complications, together with in‐hospital mortality. Since stroke mortality may impact differently by sex and is also influenced by hospital length of stay, we proceeded to stratify by these variables.ResultsSix‐hundred and seventy‐three patients were included. Overall, in‐hospital mortality rate was 7.13%. Stratifying by length of stay in‐hospital (< 7 days and ≥ 7 days), we observed that within the first week, overall mortality was related to a history of previous stroke, higher stroke severity, and to cardiovascular and respiratory complications. After 7 days, the main factor independently associated with overall in‐hospital mortality was stroke severity (National Institutes of Health Stroke Scale (NIHSS) ≥ 14, odds ratio (OR): 17.15; 95% CI, 3.06‐96.07).Stratifying by sex, we observed that females had a worse outcome if there was a history of prior stroke (OR: 3.29; 95% CI, 1.19‐9.10), higher stroke severity (NIHSS ≥ 14, OR: 16.63; 95% CI, 4.66‐59.31), and cardiovascular complications (OR: 29.70; 95% CI, 5.70‐154.8). Among men, stroke severity (NIHSS ≥ 14, OR: 23.19; 95% CI, 5.69‐94.56), respiratory infections (OR: 3.84; 95% CI, 1.32‐11.20), and older age had significant negative impact.ConclusionsStroke severity and potentially modifiable complications (respiratory infections and cardiovascular complications) confer an increased risk of in‐hospital death in both women and men, particularly during the first week of admission.
Evaluation of basin storage–discharge sensitivity in Taiwan using low‐flow recession analysis
Authors: Yeh Hsin‐Fu , Huang Chia‐Chi 
Published: February 2019

AbstractSensitivity analysis of the hydrological behaviour of basins has mainly focused on the correlation between streamflow and climate, ignoring the uncertainty of future climate and not utilizing complex hydrological models. However, groundwater storage is affected by climatic change and human activities. The streamflow of many basins is primarily sourced from the natural discharge of aquifers in upstream regions. The correlation between streamflow and groundwater storage has not been thoroughly discussed. In this study, the storage–discharge sensitivity of 22 basins in Taiwan was investigated by means of daily streamflow and rainfall data obtained over more than 30 years. The relationship between storage and discharge variance was evaluated using low‐flow recession analysis and a water balance equation that ignores the influence of rainfall and evapotranspiration. Based on the obtained storage–discharge sensitivity, this study explored whether the water storage and discharge behaviour of the studied basins is susceptible to climate change or human activities and discusses the regional differences in storage–discharge sensitivity. The results showed that the average storage–discharge sensitivities were 0.056 and 0.162 mm−1 in the northern and southern regions of Taiwan, respectively. In the central and eastern regions, the values were both 0.020 mm−1. The storage–discharge sensitivity was very high in the southern region. The regional differences in storage–discharge sensitivity with similar climate conditions are primarily due to differences in aquifer properties. Based on the recession curve, other factors responsible for these differences include land utilization, land coverage, and rainfall patterns during dry and wet seasons. These factors lead to differences in groundwater recharge and thus to regional differences in storage–discharge sensitivity.
Impact of intertwin interval on short‐term neonatal outcomes of the second twin in dichorionic pregnancies with vaginal delivery
Authors: Benito Marta , De Bonrostro Carlos , Agustín Andrea , Roca Montserrat , Campillos Jose M. , Castán Sergio 
Published: February 2019

AbstractObjectiveTo examine the effect of intertwin interval on umbilical cord pH and Apgar scores of the second twin after vaginal delivery.MethodsA retrospective study of twin deliveries at a university hospital in Spain between August 2012 and September 2017. Inclusion criteria were vaginal delivery of both twins at 32 gestational weeks or more. Exclusion criteria were monochorionic pregnancies and indication for cesarean delivery. The sample was dichotomized by intertwin interval (<10 and ≥10 minutes). Neonatal outcomes including Apgar scores and umbilical cord pH were evaluated.ResultsOverall, 323 twin deliveries were included. Intertwin interval was less than 10 minutes in 277 (85.6%) cases, and 10 minutes or longer in 46 (14.2%). There were no differences in maternal or obstetric characteristics between the groups. Incidence of instrumental delivery (P<0.001) and internal podalic version (P<0.001) for the second twin was higher in the longer interval group. A longer interval was associated with higher frequencies of 1‐minute Apgar score below 4 (P=0.009), 5‐minute Apgar score below 7 (P<0.001), and umbilical cord pH below 7.15 (P<0.001).ConclusionSecond twins with an intertwin interval of 10 minutes or longer are more likely to have poorer Apgar scores and arterial blood pH below 7.15.
Use of affinity allows anti‐inflammatory and anti‐microbial dual release that matches suture wound resolution
Authors: Haley Rebecca M. , Qian Victoria R. , Learn Greg D. , von Recum Horst A. 
Published: February 2019

AbstractSurgical sutures are vulnerable to bacterial infections and biofilm formation. At the suture site, pain and undesirable, excess inflammation are additionally detrimental to wound healing. The development of a polymerized cyclodextrin (pCD) coated surgical suture introduces the capability to locally deliver both anti‐inflammatory and anti‐microbial drugs throughout the phases of acute and chronic healing. Local delivery allows for the improvement of wound healing while reducing related systemic side effects and drug resistance. Through testing, it has been shown that the fabrication of our pCD coating minimally affects the suture's mechanical properties. In vitro studies show measurable and consistent drug delivery for nearly 5 weeks. The therapeutic level of this delivery is sufficient to show inhibition of bacterial growth for 4 weeks, and free‐radical scavenging (an in vitro anti‐inflammatory activity approximation) for 2 weeks. With this pCD coating technique, we maintain clinical performance standards while also introducing a long‐term dual delivery system relevant to the wound healing timeframe. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.
Biomechanical characterization of a novel collagen‐hyaluronan infused 3D‐printed polymeric device for partial meniscus replacement
Authors: Ghodbane Salim A. , Patel Jay M. , Brzezinski Andrzej , Lu Tyler M. , Gatt Charles J. , Dunn Michael G. 
Published: February 2019

AbstractThe menisci transmit load by increasing the contact area and decreasing peak contact stresses on the articular surfaces. Meniscal lesions are among the most common orthopedic injuries, and resulting meniscectomies are associated with adverse polycaprolactone contact mechanics changes and, ultimately, an increased likelihood of osteoarthritis. Meniscus scaffolds were fabricated by 3D‐printing a network of circumferential and radial filaments of resorbable polymer (poly(desaminotyrosyl‐tyrosine dodecyl ester dodecanoate)) and infused with collagen‐hyaluronan. The scaffold demonstrated an instantaneous compressive modulus (1.66 ± 0.44 MPa) comparable to native meniscus (1.52 ± 0.59 MPa). The scaffold aggregate modulus (1.33 ± 0.51 MPa) was within 2% of the native value (1.31 ± 0.36 MPa). In tension, the scaffold displayed a comparable stiffness to native tissue (127.6–97.1 N/mm) and an ultimate load of 33% of the native value. Suture pull‐out load of scaffolds (83.1 ± 10.0 N) was within 10% of native values (91.5 ± 15.4 N). Contact stress analysis demonstrated the scaffold reduced peak contact stress by 60–67% and increased contact area by 38%, relative to partial meniscectomy. This is the first meniscal scaffold to match both the axial compressive properties and the circumferential tensile stiffness of the native meniscus. The improvement of joint contact mechanics, relative to partial meniscectomy alone, motivates further investigation using a large animal model. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2457–2465, 2019.
A proposal for sectorial organizing and quality standards in therapeutic apheresis: The therapeutic apheresis unit (TAU) standards
Authors: Pierelli Luca , Iaconianni Vincenzo , Vacca Michele 
Published: February 2019

AbstractTherapeutic apheresis (TA) includes a wide range of therapeutic procedures based on the separation of blood components and the collection of cells with therapeutic activity or the removal of unwanted plasma or cellular components involved in the etiology of various hematologic, renal, neurological, and medical diseases. The complexity of these interventions requires an organizing model to assure a proper clinical environment, technology, quality requirements, and personnel as well as an active system for outcome monitoring for safety and efficacy. Finally, a structured organizing model may favor the efficiency of the TA unit and economic affordability. Here, we describe the more relevant characteristics of a model of TA standards, named TA unit (TAU) standards, that may help to establish a quality program in units working in the field of TA (shown as supplementary material and available at http://www.ifeit.org/pdf/TAU_Standards_3.0.pdf.
Knockdown of Sal‐like 4 expression by siRNA induces apoptosis in colorectal cancer
Authors: Hesari AmirReza , Rajab Shadi , Rezaei Marzieh , Basam Maryam , Golmohamadi Sara , Ghasemi Faezeh 
Published: February 2019

AbstractColorectal cancer (CRC) is known as the third most common malignancies among men and women and is also the second leading cause of cancer‐related deaths worldwide. It has been indicated that a variety of risk factors are involved in the pathogenesis of CRC. Spalt‐like transcription factor 4 (SALL4) is known as a transcription factor that plays an important role in the proliferation of cancerous cells. In this study, using a specific sequence of small interfering RNA (siRNA) against the sequence of SALL4, its activity is investigated in the CRC cell line (sw742). The CRC cells (sw742) were cultured and then, using a specific anti‐SALL4 siRNA, their toxic doses were determined. Then, the gene is transfected into the cell. Proliferation and expression of the SALL4 and Bcl‐2 gene were measured using the real‐time polymerase chain reaction method. Cell death was evaluated by propidium iodide staining and fluorescence‐activated cell sorting analysis. Our results indicated that the specific concentration of siRNA of the SALL4 gene was 62.5 nmole. Gene expression of SALL4 and Bcl‐2 results showed that expression of Bcl‐2 gene in the siRNA group was significantly reduced. In conclusion, our finding indicated that it could be used as a therapeutic and diagnostic biomarker in the treatment of patients with CRC.
Effects of SRSF1 on subnuclear localization of topoisomerase I
Authors: Girstun Agnieszka , Ishikawa Takao , Staron Krzysztof 
Published: February 2019

AbstractSubnuclear localization of topoisomerase I (top I) is determined by its DNA relaxation activity and a net of its interactions with in majority unidentified nucleolar and nucleoplasmic elements. Here, we recognized SR protein SRSF1 (Serine/arginine‐rich splicing factor 1, previously known as SF2/ASF) as a new element of the net. In HeLa cells, overexpression of SRSF1 recruited top I to the nucleoplasm whereas its silencing concentrated it in the nucleolus. Effect of SRSF1 was independent of top I relaxation activity and was the best pronounced for the mutant inactive in relaxation reaction. In HCT116 cells where top I was not released from the nucleolus upon halting relaxation activity, it was also not relocated by elevated level of SRSF1. Out of remaining SR proteins, SRSF5, SRSF7, and SRSF9 did not influence the localization of top I in HeLa cells whereas overexpression of SRSF2, SRSF3, SRSF6, and partly SRSF4 concentrated top I in the nucleolus, most possibly due to the reduction of the SRSF1 accessibility. Specific effect of SRSF1 was exerted because of its distinct RS domain. Silencing of SRSF1 compensated the deletion of the top I N‐terminal region, individually responsible for nucleoplasmic localization of the mutant, and restored the wild‐type phenotype of deletion mutant localization. SRSF1 was essential for the camptothecin‐induced clearance from the nucleolus. These results suggest a possible role of SRSF1 in establishing partition of top I between the nucleolus and the nucleoplasm in some cell types with distinct combinations of SR proteins levels.
Natural compounds with DPP‐4 inhibitory effects: Implications for the treatment of diabetes
Authors: Yaribeygi Habib , Atkin Stephen L. , Sahebkar Amirhossein 
Published: February 2019

AbstractDipeptidyl peptidase‐4 (DPP‐4) inhibitors are antidiabetes agents that decrease blood glucose by preventing the degradation of endogenous glucagon‐like peptide‐1. The first DPP‐4 was sitagliptin followed by several other agents in the class introduced to manage diabetes. Recent studies have suggested that naturally occurring compounds can exert an antidiabetes effect through DPP‐4 inhibition. Such compounds may have a place in the treatment of diabetes within the diet; however, while DPP‐4 inhibition alone is not associated with hypoglycemia, in combinations with other medication hypoglycemia can result, therefore, it is critical to know what herbal or food‐based compounds may have these activities in the management of diabetes patients. In this review, we have outlined the compounds that have DPP‐4 inhibition that may have utility in the treatment of diabetes.
Occult hepatitis C virus infection in patients with beta‐thalassemia major: Is it a neglected and unexplained phenomenon?
Authors: Ayadi Ahmad , Nafari Amir Hossein , Irani Shiva , Mohebbi Elham , Mohebbi Fahimeh , Sakhaee Fatemeh , Vaziri Farzam , Siadat Seyed Davar , Fateh Abolfazl 
Published: February 2019

AbstractOccult hepatitis C virus (HCV) infection (OCI) is described as the presence of viral genome in both hepatocytes and peripheral blood mononuclear cells (PBMCs) despite constant negative results on serum HCV RNA tests. Beta‐thalassemia major (BTM) describes a group of inherited blood diseases. Patients with BTM require repeated blood transfusions, increasing the risk of exposure to infectious agents. We aimed to assess the prevalence of OCI in Iranian BTM patients and to identify the role of host factors in OCI positivity. A total of 181 BTM patients with HCV negative markers were selected. HCV RNA was tested in PBMCs using nested polymerase chain reaction assay. The positive samples were then genotyped via restriction fragment‐length polymorphism (RFLP) and 5′‐untranslated region sequencing. Six (3.3%) out of 181 BTM patients had viral HCV genomes in PBMC samples. Three (50.0%), two (33.3%), and one (16.7%) out of these six patients were infected with HCV‐1b, HCV‐1a, and HCV‐3a, respectively. OCI positivity was significantly associated with the serum level of uric acid (P = 0.045) and ABO blood group (P = 0.032). Also, OCI patients had unfavorable IFNL3 rs12979860 TT, IFNL3 rs8099917 GG, IFNL3 rs12980275 GG, and IFNL4 ss469415590 ∆G/∆G genotypes. In conclusion, we indicated the low frequency of OCI in BTM patients. Nevertheless, more attention is warranted considering the importance of this infection. Also, further studies are necessary to determine the actual prevalence of OCI among BTM patients in Iran.
Psychometric properties of the self‐sufficiency matrix among homeless and vulnerably housed individuals and families
Authors: Cummings Camilla , Brown Molly 
Published: February 2019

AbstractHomeless service systems have been implementing assessment tools to inform service provision for individuals and families. Although a variety of assessments are available, their psychometric evidence base is limited. The self‐sufficiency matrix (SSM) is one assessment that demonstrates promising reliability and validity. However, the SSM's factor structure has been inconsistent across studies. This study explored the factor structure of the SSM, using exploratory factor analysis and confirmatory factor analysis in a sample of individuals (N = 427) and families (
N = 428) at risk of or experiencing homelessness. Data were derived from a midwestern city's Homeless Management Information System and included all participants from the Homelessness Prevention and Rapid Re‐Housing Program. Results suggest the SSM is multidimensional and the factor structure differs across individuals and families. Additionally, the SSM demonstrates measurement invariance across racial and gender groups. Further development and testing of the SSM is necessary to better serve individuals experiencing homelessness.
Retracted: Tetramethylpyrazine partially relieves hypoxia‐caused damage of cardiomyocytes H9c2 by downregulation of miR‐449a
Authors: Zhang Xuesong , Dong Huawei , Liu Yong , Han Junxia , Tang Shouyi , Si Jingna 
Published: February 2019

AbstractInadequate oxygen supply is probably one of the most important pathophysiological mechanisms of cardiomyocyte damage in ischemic heart disease. Tetramethylpyrazine (TMP, also known as ligustrazine) is the main active ingredient isolated from the rhizome of Ligusticum chuanxiong Hort. A previous study reported that the TMP could exert cardioprotective activity. This study aimed to explore the molecular mechanism of the protective effects of TMP on cardiomyocyte damage caused by hypoxia. The viability and apoptosis of cardiomyocytes H9c2 were detected using cell counting kit‐8 assay and annexin V‐FITC/PI staining, respectively. Quantitative reverse transcription polymerase chain reaction was conducted to measure the expression level of microRNA‐449a (miR‐449a). Cell transfection was performed to upregulate the expression level of miR‐449a or downregulate the expression level of sirtuin 1 (Sirt1). The protein expression levels of Sirt1 and key factors involved in cell apoptosis and phosphatidylinositol 3‐kinase/protein kinase 3 (PI3K/AKT) pathway were evaluated using western blot analysis. We found that the hypoxia incubation inhibited H9c2 viability, induced cell apoptosis, and inactivated the PI3K/AKT pathway. TMP treatment partially relieved the hypoxia‐caused H9c2 cell viability loss and apoptosis, as well as reversed the hypoxia‐caused inactivation of the PI3K/AKT pathway. Moreover, TMP partially alleviated the upregulation of miR‐449a in H9c2 cells caused by hypoxia. Overexpression of miR‐449a weakened the effects of TMP on hypoxia‐treated H9c2 cells. Furthermore, Sirt1 was a target gene of miR‐449a. Knockdown of Sirt1 also weakened the effects of TMP on hypoxia‐treated H9c2 cells. In conclusion, TMP partially relieved hypoxia‐caused cardiomyocytes H9c2 viability loss and apoptosis at least through downregulating miR‐499a, upregulating Sirt1, and then activating the PI3K/AKT pathway.