bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knocking down of LINC01220 inhibits proliferation and induces apoptosis of endometrial carcinoma through silencing MAPK11
Authors: Li Yong , Kong Chengcai , Wu Chaoying , Wang Yingqiao , Xu Boqun , Liang Shenglian , Ying Xiaoyan 
Published: May 2019

Abstract
Background: Endometrial carcinoma (EC) still threatens the health of women. Thus, to explore how long intergenic non-protein coding RNA 01220 regulates the development of EC.
Methods: Whole genome expression profile data of EC and paracancerous tissues in TCGA database were downloaded. LINC01220 expression in EC and paracancerous tissues of patients in our hospital were detected by qRT-PCR. Furthermore, the relationship between LINC01220 expression and clinicopathological features of EC patients was analyzed. After transfection with sh-LINC01220 and pcDNA-MAPK11 (mitogen-activated protein kinase) in EC cells, proliferative, colony formation abilities and apoptosis were determined by cell counting kit-8 (CCK-8), colony formation assay and flow cytometry, respectively. Western blot was conducted to determine the regulatory role of LINC01220 on MAPK11.
Results: TCGA data showed that LINC01220 expression is markedly higher in EC tissues than that of paracancerous tissues, which was consistent without detection in EC patients of our hospital. LINC01220 expression was positively correlated to pathological grade and International Federation of Gynecology and Obstetrics (FIGO) stage of EC patients. After knockdown of LINC01220 in EC cells, proliferative and colony formation abilities decreased, whereas apoptotic rate increased. Cor function analysis revealed the positive correlation between LINC01220 and MAPK11 in EC. MAPK11 expression was regulated by LINC01220 in EC cells. Overexpression of MAPK11 can reverse the tumor suppressing effect of LINC01220 on EC.
Conclusions: LINC01220 promotes EC development by stimulating proliferation and inhibiting apoptosis of EC cells through up-regulating MAPK11.
Knockdown of hsa_circ_0023028 inhibits cell proliferation, migration, and invasion in laryngeal cancer by sponging miR-194-5p
Authors: Chen Xiaofeng , Su Xiaoqing , Zhu Chuansai , Zhou Jing 
Published: May 2019

Abstract
Emerging evidences have proposed that circular RNAs (circRNAs) play a major role in carcinogenesis. Hsa_circ_0023028 has been reported to be aberrantly expressed in laryngeal cancer (LCa). However, the role and the mechanism of hsa_circ_0023028 in LCa have not been adequately studied. In the present study, we demonstrated that hsa_circ_0023028 expression was up-regulated in LCa tissues and cell lines. miR-194-5p was down-regulated in LCa cells. Functionally, knockdown of hsa_circ_0023028 inhibited the proliferation, migration, and invasion of LCa cells, as evidenced by the reduced number of 5-Ethynyl-2′-deoxyuridine (EdU)-positive cells and decreased number of migrated and invaded cells. Additionally, hsa_circ_0023028 was identified as an miR-194-5p sink. A negative correlation between miR-194-5p and hsa_circ_0023028 expression was observed in LCa tissues. Besides, down-regulation of miR-194-5p attenuated the inhibitory effects of hsa_circ_0023028 silencing on LCa cell proliferation, migration, and invasion. In summary, hsa_circ_0023028 functions as an miR-194-5p sponge to promote the proliferation, migration, and invasion of LCa cells.
The treatment role of Cyperus rotundus L. to triple-negative breast cancer cells
Authors: Wang Fukai , Song Xiang , Ma Shuangshuang , Liu Chenyu , SUN Xiaohui , Wang Xinzhao , Liu Zhaoyun , Liang Dong , Yu Zhiyong 
Published: May 2019

Abstract
Cyperus rotundus L. is widely used in Traditional Chinese Medicine and studies have reported its anticancer effect, but its chemical composition and therapy mechanism remains unknown. This research aims to analyze the chemical components of the ethanol extract of Cyperus rotundus L. (EECR), detect its treatment effects on human Triple-negative breast cancer (TNBC) cells, and elucidate possible therapy mechanisms. The chemical components of EECR were detected by the Waters UPLC combined with Bruker Q-TOF mass spectrometer (UPLC-Q-TOF-MS). The phytochemical compounds were identified by comparing the mass fragmentations of each metabolite with databases such as METLIN, HMDB, and NCBI. A total of 21 compounds were identified in EECR. MDA-MB-231 and MDA-MB-468 cells were treated with various concentrations of EECR. Cell proliferation was examined using Cell Counting Kit-8 (CCK-8) and colony formation assays. Cell apoptosis and cell cycle were detected by flow cytometry. Apoptosis- and autophagy-related protein expression was detected by Western blot. EECR inhibits the proliferation of TNBC cells (MDA-MB-231 and MDA-MB-468) in a dose-dependent manner, which may be related to the arrest of cell cycle in G0/G1 phase. It induces apoptosis by promoting the expression of BAX and inhibiting the expression of BCL-2. In addition, autophagy inhibitor 3-Methyladenine (3-MA) inhibited TNBC cells pro-survival autophagy and increased the sensitivity of EECR. The present results demonstrated that EECR has potential effects on inhibits the proliferation and induction apoptosis in TNBC.
Analysis of partial discharge sources in stator insulation system using variable excitation frequency
Authors: Nair Ramesh P. , Vishwanath Sumangala B. 
Published: May 2019

Identification of partial discharge (PD) sources in stator insulation is an important and challenging task. Distinct phase‐resolved PD (PRPD) pattern of different PD sources are well established at power frequency (50/60 Hz) and can help to identify each source. In this study, different sources of PD commonly found in stator insulation were created on a 6.6 kV epoxy‐mica stator coil. Each PD source was experimentally investigated at a variable excitation frequency in the range of 50–0.1 Hz. PRPD pattern and PD parameters such as integrated charge per cycle and number of discharge pulses per cycle of each discharge source were compared at different frequencies. The comparison suggests that the parameter of each PD source was dependent on excitation frequency. Some discharge sources have shown a distinct variation in their characteristics with excitation frequency. These distinct characteristics are additional information to the existing ones, which will help to correlate PD characteristics with the source of PD in stator insulation.
Dust of comet 67P/Churyumov-Gerasimenko collected by Rosetta/MIDAS: classification and extension to the nanometer scale
Authors: Mannel T. , Bentley M. S. , Boakes P. D. , Jeszenszky H. , Ehrenfreund P. , Engrand C. , Koeberl C. , Levasseur-Regourd A. C. , Romstedt J. , Schmied R. , Torkar K. , Weber I. 
Published: May 2019

Context. The properties of the smallest subunits of cometary dust contain information on their origin and clues to the formation of planetesimals and planets. Compared to interplanetary dust particles or particles collected during the Stardust mission, dust collected in the coma of comet 67P/Churyumov-Gerasimenko (67P) during the Rosetta mission provides a resource of minimally altered material with known origin whose structural properties can be used to further the investigation of the early solar system.
Aims. The cometary dust particle morphologies found at comet 67P on the micrometer scale are classified, and their structural analysis is extended to the nanometer scale.
Methods. We present a novel method for achieving the highest spatial resolution of imaging obtained with the MIDAS Atomic Force Microscope on board Rosetta. 3D topographic images with resolutions down to 8 nm were analyzed to determine the subunit sizes of particles on the nanometer scale.
Results. Three morphological classes can be determined: (i) fragile agglomerate particles of sizes larger than about 10 μm comprised of micrometer-sized subunits that may themselves be aggregates and show a moderate packing density on the surface of the particles. (ii) A fragile agglomerate with a size of about a few tens of micrometers comprised of micrometer-sized subunits that are suggested to be aggregates themselves and are arranged in a structure with a fractal dimension lower than two. (iii) Small micrometer-sized particles comprised of subunits in the size range of hundreds of nanometers that show surface features that are again suggested to represent subunits. Their differential size distributions follow a log-normal distribution with means of about 100 nm and standard deviations between 20 and 35 nm.
Conclusions. The properties of the dust particles found by MIDAS represent an extension of the dust results of Rosetta to the micro- and nanometer scale. All micrometer-sized particles are hierarchical dust agglomerates of smaller subunits. The arrangement, appearance, and size distribution of the smallest determined surface features are reminiscent of those found in chondritic porous interplanetary dust particles. They represent the smallest directly detected subunits of comet 67P.
SHALOS: StatisticalHerschel-ATLAS lensed objects selection
Authors: González-Nuevo J. , Suárez Gómez S. L. , Bonavera L. , Sánchez-Lasheras F. , Argüeso F. , Toffolatti L. , Herranz D. , González-Gutiérrez C. , García Riesgo F. , de Cos Juez F. J. 
Published: May 2019

Context. The statistical analysis of large sample of strong lensing events can be a powerful tool to extract astrophysical or cosmological valuable information. Their selection using submillimetre galaxies has been demonstrated to be very effective with more than ∼200 proposed candidates in the case ofHerschel-ATLAS data and several tens in the case of the South Pole Telescope. However, the number of confirmed events is still relatively low, i.e. a few tens, mostly because of the lengthy observational validation process on individual events.Aims. In this work we propose a new methodology with a statistical selection approach to increase by a factor of ∼5 the number of such events within theHerschel-ATLAS data set. Although the methodology can be applied to address several selection problems, it has particular benefits in the case of the identification of strongly lensed galaxies: objectivity, minimal initial constrains in the main parameter space, and preservation of statistical properties.Methods. The proposed methodology is based on the Bhattacharyya distance as a measure of the similarity between probability distributions of properties of two different cross-matched galaxies. The particular implementation for the aim of this work is called SHALOS and it combines the information of four different properties of the pair of galaxies: angular separation, luminosity percentile, redshift, and the ratio of the optical to the submillimetre flux densities.Results. The SHALOS method provides a ranked list of strongly lensed galaxies. The number of candidates within ∼340 deg2of theHerschel-ATLAS surveyed area for the final associated probability,Ptot >  0.7, is 447 and they have an estimated mean amplification factor of 3.12 for a halo with a typical cluster mass. Additional statistical properties of the SHALOS candidates, as the correlation function or the source number counts, are in agreement with previous results indicating the statistical lensing nature of the selected sample.
Predictors of a Successful Bipolar Radiofrequency Endometrial Ablation
Authors: Eisele Lilly , Köchli Lea , Städele Patricia , Welter JoEllen , Fehr-Kuhn Maja , Fehr Mathias 
Published: May 2019

Abstract
Introduction The studyʼs objectives were to determine the success rate following radiofrequency endometrial ablation to treat abnormal menstrual bleeding and to assess risk factors for failure of the method.
Materials and Methods 195 women who were treated with bipolar radiofrequency endometrial ablation between 01/2009 and 06/2016 were included in this prospective cohort study. Postoperative data from 187 women were collected at a median of 17.5 months (IQR 4.5–34.9; 1–82). Multivariate analyses of risk factors were performed. Success was defined as amenorrhoea or spotting.
Results Patient characteristics were as follows: mean age 44 years (SD ± 5), median parity 2 (IQR 2–3), median hysterometer 8.7 cm (SD ± 1.1), and median BMI 23.5 kg/m2 (IQR 21–27). 30 patients (19.5%) had intramural masses that could be measured with ultrasound. Postoperative success rate was 86.1%. 10 patients (5%) had a hysterectomy postoperatively – 6 for heavy bleeding, 3 due to prolapse, and 1 due to dysmenorrhoea. Multivariate analyses showed the presence of intramural masses in women < 45 years was a significant risk factor for therapeutic failure (p = 0.033; 95% CI 1.08–12.57), with an increased risk of hysterectomy (OR 7.9, 95% CI 1.2–52.7, p = 0.033).
Conclusion Bipolar radio frequency endometrial ablation was highly successful in the absence of an intramural mass (88%). Even smaller intramural fibroids (DD: adenomyomas of a median of 15 mm) reduce the success rate (76%), which is why preoperative ultrasound is recommended. In the presence of intramural masses, the risk of a hysterectomy for women < 45 years increases eightfold.
Female Adnexal Tumour of Probable Wolffian Origin (FATWO): Review of the Literature
Authors: Hübner Theresa , Schwab Michael , Hartmann Elena , Mayr Doris , Thurner Annette , Bley Thorsten , Wöckel Achim , Herr Daniel 
Published: May 2019

AbstractFATWO (female adnexal tumour of probable Wolffian origin) denotes a rare tumour of the female adnexa which has been described in nearly 80 cases worldwide and which presumably originates in the remains of the Wolffian ducts. In 10 to 20 percent of patients, a metastasis or recurrence was seen subsequently and for this reason, a malignant potential is attributed to the FATWO. Because of the small number of cases, there is no clear therapeutic recommendation. The method of choice currently is surgical treatment with hysterectomy and adnexectomy. The benefit of radio- and chemotherapies is not clear and a small number of treatment attempts with imatinib have been made in cases of CD117 positivity or treatment attempts on an endocrine basis. This work provides an overview of the literature on epidemiology, imaging and histopathological diagnostic features as well as therapeutic options of this same tumour form.
Postpartum Depressive Disorder – Psychosomatic Aspects
Authors: Schipper-Kochems Stephanie , Fehm Tanja , Bizjak Gabriele , Fleitmann Ann , Balan Percy , Hagenbeck Carsten , Schäfer Ralf , Franz Matthias 
Published: May 2019

AbstractPostpartum depression (PPD) is the most common mental illness in mothers following the birth of a child. Since the symptoms of PPD are similar to the normal stress of healthy women following childbirth, it is often difficult for the attending gynaecologist or midwife to diagnose this illness in a timely manner and thus initiate adequate treatment and comprehensive support for the patient. Even if there are options for a screening using evaluated questionnaires and subsequent psychotherapy and/or drug therapy in the treatment of PPD which has proven effective, it is seen that, in most treatment approaches, little consideration is given to the affect-controlled interaction and the bonding behaviour between mother and child. This article presents diagnostic measures and current therapeutic approaches as well as their integration in practice in order to achieve awareness of this topic in everyday clinical practice and show the pathways of appropriate treatment. Specific multiprofessional treatment approaches which centre on the mother-child relationship demonstrate successes with regard to depression in the mothers and also on the development of a secure mother-child bond and are thus a protective factor in the development of the affected children. The now well-known effects of PPD on the fathers as well as the negative impacts of paternal depression on child development make it clear that the treatment should not focus solely on maternal depression, but also always on the family bond between the mother, child and father in the treatment.
Unterschiedliche Häufigkeiten pneumologischer Krankheitsbilder in der ambulanten und stationären Medizin
Authors: Herkenrath S. , Mülleneisen N. , Treml M. , Kietzmann I. , Hagmeyer L. , Randerath W. 
Published: May 2019

Zusammenfassung
Hintergrund Die Evaluation von Behandlungsschwerpunkten in der stationären und ambulanten pneumologischen Patientenversorgung ist von besonderem gesundheitspolitischem Interesse, da sie Auswirkungen auf Arzt, Patient und die Forschung hat. Diese Arbeit beschreibt Unterschiede und Gemeinsamkeiten der Behandlungsschwerpunkte heutiger ambulanter und stationärer pneumologischer Patientenversorgung und diskutiert deren Auswirkungen auf Patient, Arzt und Forschung.
Methodik Der Vergleich zwischen ambulantem und stationärem Sektor erfolgt in der hier vorliegenden Studie auf Basis der Häufigkeiten verschlüsselter ICD-Codes einer pneumologischen Fachklinik (5211 Fälle des Jahres 2016) sowie der seitens der kassenärztliche Vereinigung Nordrhein veröffentlichten häufigsten ICD-10-Codes pneumologischer Arztpraxen im dritten Quartal 2016 (142431 Fälle).
Ergebnisse Viele pneumologische Krankheitsbilder werden in relativ ähnlichen Anteilen sowohl in der Praxis als auch im Krankenhaus behandelt. Bei anderen Krankheitsbildern zeigen sich erhebliche Unterschiede in der relativen Häufigkeit in Praxis und Klinik. So werden allergische Erkrankungen wie die allergische Rhinopathie und das Asthma bronchiale v. a. ambulant behandelt, während die respiratorische Insuffizienz und das Lungenkarzinom vorwiegend eine Domäne der stationären pneumologischen Medizin sind.
Schlussfolgerung Die Behandlungsschwerpunkte in der ambulanten und stationären pneumologischen Versorgung unterscheiden sich ungeachtet vieler Gemeinsamkeiten dennoch zum Teil erheblich. Dies hat Auswirkungen auf die ärztliche Weiterbildung, die Durchführung klinischer Studien, aber auch und v. a. auf die Patientenversorgung. Zur Erhaltung eines hohen medizinischen Versorgungsniveaus in allen Bereichen der Pneumologie scheint daher ein enger inhaltlicher Austausch zwischen ambulantem und stationärem Sektor unverzichtbar. Von einer sektorenübergreifenden Verfügbarkeit medizinischer Expertise profitieren am Ende alle: Ärzte, Patienten und Wissenschaft.
Surgical and Non-surgical Treatment of Vertebral Fractures in Elderly
Authors: Chmielnicki Marc , Prokop Axel , Kandziora Frank , Pingel Andreas 
Published: May 2019

AbstractDemographic development in Germany has led to an aging of the population. Particularly for these patients, osteoporosis-induced vertebral fractures represent a significant decrease in quality of life and level of activity. According to current guidelines, the initial treatment of stable osteoporotic vertebral fractures is conservative management with analgesic, anti-osteoporotic, physical therapy, and orthotic measures as first line options. Personal experience, however, suggests that patients benefit from timely surgical treatment through rapid improvement of pain symptoms and thus, more rapid mobilization. The poor bone quality of elderly patients presents the treating spine surgeon a challenge in achieving stable spinal fusion with or without support, for example, through augmentation. Minimally invasive procedures have increasingly established themselves for such purposes in recent years. With over 1000 fracture treatments in the last 3.5 years, we have developed a differentiated treatment concept depending on patient age and fracture morphology, which we would like to introduce. Unstable fractures with posterior edge involvement are stabilized from posterior with a percutaneous fixator. Patients over 60 years were treated percutaneously with a polyaxial screw system. Increased stability was achieved by PMMA cement augmentation of the fenestrated screws. In elderly patients with Magerl A3 fractures without neurologic deficit, the index vertebra is supplementally treated with kyphoplasty (hybrid treatment). In acute, stable osteoporotic vertebral fractures with severe pain despite analgesics, we perform kyphoplasty, which is possible even in high thoracic fractures to T3 with smaller balloons and thinner trocars. Vertebroplasty is another option in the lumbar and lower thoracic spine. Because of invasiveness, extended posterior–anterior correction procedures are generally avoided in this population, which has frequent multiple comorbidities.
Serum Vitamin D Levels in Fertile and Infertile Women with Polycystic Ovary Syndrome
Authors: Kokanalı Demet , Karaca Mujdegul , Ozakşit Gulnur , Elmas Burak , Engin Üstün Yaprak 
Published: May 2019

Abstract
Introduction In polycystic ovary syndrome, serum vitamin D levels are known to correlate with metabolic conditions such as diabetes mellitus, metabolic syndrome and cardiovascular disease. However, there are not enough studies showing such a relationship with female fertility. We aimed to compare serum vitamin D levels in fertile and infertile women with polycystic ovary syndrome to evaluate whether vitamin D may play a role in the pathogenesis of fertility problems in women with polycystic ovary syndrome.
Materials and Methods 274 infertile and 111 fertile women with polycystic ovary syndrome were included in this retrospective study. Infertile and fertile groups were matched by age, body mass index and homeostasis model assessment of insulin resistance. Anthropometric, clinical and laboratory characteristics of the women were recorded. Serum 25(OH)D3 levels were used to assess serum vitamin D levels.
Results No significant differences were detected between groups in terms of anthropometric, clinical and laboratory features except for serum 25(OH)D3 levels and the incidence of vitamin D deficiency. Vitamin D levels were significantly lower and vitamin D deficiency was more common in the infertile group compared to the fertile group. When the groups were stratified into obese/non-obese or insulin resistance positive/negative, infertile obese and infertile insulin resistance-positive women had the lowest serum 25(OH)D3 levels.
Conclusion Serum vitamin D levels are lower in infertile women with polycystic ovary syndrome compared to fertile women. When insulin resistance or obesity was present, vitamin D levels were reduced further. Thus, in polycystic ovary syndrome, lower vitamin D levels may play a role in the pathogenesis of fertility problems.