bluuebunny/crossref_metadata_2025_split huggingface dataset:
Placental and breastmilk transfer of voriconazole to offspring from pregnant and lactating bottlenose dolphins (Tursiops truncatus)
Authors: Ohno Yoshito , Kobayashi Marisa , Akune Yuichiro , Inoshima Yasuo 
Published: July 2019

AbstractFungal pneumonia is a common disease in bottlenose dolphins (Tursiops truncatus), including pregnant and lactating ones. Voriconazole (VRCZ) is commonly used to treat respiratory fungal infections in this species; however, it is unknown whether VRCZ is transferred via the placenta and breastmilk and whether its usage is safe in pregnant and lactating dolphins. We measured VRCZ concentrations in breastmilk and dams’, umbilical cord, and calves’ plasma samples from four dam-calf dolphin pairs in the Port of Nagoya Public Aquarium, Japan, treated with or without VRCZ. Three pregnant and/or lactating dams were administered VRCZ (loading dose 1.5–2.3 mg/kg, for 3 days; maintenance dose 1.5–3.1 mg/kg, every 5–18 days), twice daily, orally, without side effects in dams or calves. VRCZ was detected in two dams’ umbilical cord plasma (0.14 and 2.35 μg/ml) and in one calf's plasma (0.18 μg/ml), collected immediately after birth. Further, VRCZ was detected in breastmilk samples (maximum 13.45 μg/ml) from three VRCZ-administered dams and in plasma from three calves (maximum 7.54 μg/ml) given or nursed from VRCZ-administered dams’ breastmilk. The calves’ plasma VRCZ concentrations varied, depending on the amount of breastmilk and food consumed. VRCZ concentrations were higher in breastmilk samples than in dams’ plasma. To our knowledge, this is the first report on placental and breastmilk VRCZ transfer to offspring in bottlenose dolphins. During VRCZ medication in pregnant and lactating bottlenose dolphins, it is crucial to monitor plasma VRCZ concentrations and any side effects in dams as well as in their calves.
Is the dark-matter halo spin a predictor of galaxy spin and size?
Authors: Jiang Fangzhou , Dekel Avishai , Kneller Omer , Lapiner Sharon , Ceverino Daniel , Primack Joel R , Faber Sandra M , Macciò Andrea V , Dutton Aaron A , Genel Shy , Somerville Rachel S 
Published: July 2019

ABSTRACT
The similarity between the distributions of spins for galaxies (λgal) and for dark-matter haloes (λhalo), indicated both by simulations and observations, is naively interpreted as a one-to-one correlation between the spins of a galaxy and its host halo. This is used to predict galaxy sizes in semi-analytic models via Re ≃ fjλhaloRvir, where Re is the half-mass radius of the galaxy, fj is the angular momentum retention factor, and Rvir is the halo radius. Using two suites of zoom-in cosmological simulations, we find that λgal and the λhalo of its host halo are in fact barely correlated, especially at z ≥ 1, in line with previous indications. Since the spins of baryons and dark matter are correlated at accretion into Rvir, the null correlation in the end reflects an anticorrelation between fj and λhalo, which can arise from mergers and a ‘wet compaction’ phase that many high-redshift galaxies undergo. It may also reflect that unrepresentative small fractions of baryons are tapped to the galaxies. The galaxy spin is better correlated with the spin of the inner halo, but this largely reflects the effect of the baryons on the halo. While λhalo is not a useful predictor for Re, our simulations reproduce a general relation of the form of Re = ARvir, in agreement with observational estimates. We find that the relation becomes tighter with A = 0.02(c/10)−0.7, where c is the halo concentration, which in turn introduces a dependence on mass and redshift.
Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis
Authors: Taochy Christelle , Yu Agnès , Bouché Nicolas , Bouteiller Nathalie , Elmayan Taline , Dressel Uwe , Carroll Bernard J , Vaucheret Hervé 
Published: July 2019

Abstract
Spontaneous post-transcriptional silencing of sense transgenes (S-PTGS) is established in each generation and is accompanied by DNA methylation, but the pathway of PTGS-dependent DNA methylation is unknown and so is its role. Here we show that CHH and CHG methylation coincides spatially and temporally with RDR6-dependent products derived from the central and 3′ regions of the coding sequence, and requires the components of the RNA-directed DNA methylation (RdDM) pathway NRPE1, DRD1 and DRM2, but not CLSY1, NRPD1, RDR2 or DCL3, suggesting that RDR6-dependent products, namely long dsRNAs and/or siRNAs, trigger PTGS-dependent DNA methylation. Nevertheless, none of these RdDM components are required to establish S-PTGS or produce a systemic silencing signal. Moreover, preventing de novo DNA methylation in non-silenced transgenic tissues grafted onto homologous silenced tissues does not inhibit the triggering of PTGS. Overall, these data indicate that gene body DNA methylation is a consequence, not a cause, of PTGS, and rule out the hypothesis that a PTGS-associated DNA methylation signal is transmitted independent of a PTGS signal.
EVALUATION OF RADIATION DOSES AND HAZARD INDICES IN CRUSHED ROCKS FROM SOME QUARRIES IN IBADAN SOUTHWESTERN NIGERIA
Authors: Alausa S K , Eluyera F O , Coker J O 
Published: July 2019

Abstract
The radiological assessment of rock samples from four quarry sites in Ibadan have been carried out. The mean activity concentrations of 40K, 226Ra and 232Th were 377.0 ± 37.9, 17.7 ± 3.6 and 4.98 ± 2.1 Bq kg−1, respectively, for Ratcon Quarry (RQ); 408.0 ± 28.7, 16.6 ± 1.6 and 10.6 ± 2.3 Bq kg−1, respectively, for Dekit Quarry; 388.0 ± 26.6, 15.7 ± 1.9 and 9.85 ± 3.9 Bq kg−1, respectively, for Hajaig Quarry; and 388.084 ± 55.9, 16.2 ± 3.9 and 12.1 ± 3.8 Bq kg−1, respectively, for NSCE Quarry (NQ). The highest mean outdoor effective dose of 0.038 ± 0.005 mSv y−1 and the minimum of 0.033 ± 0.004 mSv y−1 were obtained at NQ and RQ, respectively. The radiological hazard indices recorded in each site were &lt;1.0. The results show lower radioactivity levels than the world average values; therefore, no serious radiological health effect is expected when the crushed rocks from the quarries are used for buildings construction.
“A Friendly Place to Grow as an Educator”: A Qualitative Study of Community and Relationships Among Medical Student Coaches
Authors: Sheu Leslie , Hauer Karen E. , Schreiner Katherine , van Schaik Sandrijn M. , Chang Anna , O’Brien Bridget C. 
Published: July 2019

Purpose
The rise of coaching programs in medical education sparks questions about ways to support physician coaches in learning new educational practices specific to coaching. How coaches learn from one another is of particular interest considering the potential value of social learning. Using communities of practice as a conceptual framework, the authors examine the sense of community and relationships among coaches in a new medical student coaching program, the value of this community, and the facilitators and barriers influencing community development.

Method
In this qualitative study, investigators conducted 34 interviews with physician coaches at 1 institution over 2 years (2017–2018) and observed 36 coach meetings. Investigators analyzed interview transcripts using thematic analysis and used observation field notes for context and refinement of themes.

Results
Coaches described a sense of community based on regular interactions; shared commitment to medical education; and new roles with similar experiences, joys, and challenges. They valued the sense of camaraderie and support, learning from one another, and opportunities for professional growth that strengthened their identities as educators and enhanced job satisfaction. Facilitators of community included regular meetings, leadership and administrative support, and informal opportunities to interact outside of meetings. Barriers included time constraints and geographic challenges for coaches at off-site locations.

Conclusions
The sense of community among coaches was a valued and beneficial part of their coaching experience. Coaches’ interactions and relationships promoted skill acquisition, knowledge transfer, professional development, and career satisfaction. Thus, incorporating support for social learning in coaching programs promotes coach faculty development.
Uncertainty in Decision Making in Medicine: A Scoping Review and Thematic Analysis of Conceptual Models
Authors: Helou Marieka A. , DiazGranados Deborah , Ryan Michael S. , Cyrus John W. 
Published: July 2019

Purpose
The practice of medicine is rarely straightforward. Data used to facilitate medical decision making may be conflicting, ambiguous, or scarce, and providing optimal care requires balancing clinicians’ expertise and available evidence with patients’ preferences. To explore uncertainty in decision making across disciplines, the authors performed a scoping review and thematic analysis of the literature to formulate a model describing the decision-making process in medicine under uncertain conditions.

Method
In 2016, the authors performed a comprehensive search of key databases using a combination of keywords and controlled vocabulary. They identified and reviewed 3,398 records. After applying their inclusion and exclusion criteria to the titles and abstracts and then full texts, 19 articles were selected. The authors applied a qualitative thematic analysis to these articles, using codes to extract themes related to uncertainty in decision making.

Results
The 19 articles spanned 6 fields of study and 5 disciplines within the health sciences. The thematic analysis revealed 6 main themes: recognition of uncertainty, classification of uncertainty, stakeholder perspectives, knowledge acquisition, decision-making approach, and evaluation of the decision-making process.

Conclusions
Based on the themes that emerged from their thematic analysis of the literature characterizing the effects of uncertainty and ambiguity on the decision-making process, the authors developed a framework depicting the interplay between these themes with a visual representation of the decision-making process under uncertain conditions. Future research includes further development and validation of this framework to inform medical school curricula.
Type 2 diabetes and depression in the African American population
Authors: Ajuwon Abidemi Mary , Love Rene 
Published: July 2019

ABSTRACT

Background and purpose:
Type 2 diabetes mellitus (T2DM) affects 30.3 million people (9.4%) in the United States. African Americans are twice as likely to be diagnosed with diabetes and have two to four times the rates of T2DM-associated complications. Depression has long been associated with poor outcomes of diabetic self-management and glycemic control. Comorbidity of T2DM and depression worsen effective self-management of these conditions in the African American population. The purpose of the study was to synthesize the literature with practice recommendations of care related to T2DM and depression in the African American population.

Methods:
A literature search was conducted using PRISMA in June 2018 with PubMed, Google Scholar, Cochrane, Scopus, Embase, and PsycINFO databases using the years from 2008 through 2018. The following terms and combination of terms were used to identify articles for the review: (a) diabetes mellitus, type 2, (b) diabetes type 2 and depression, and (c) diabetes mellitus type 2, depression, African America.

Conclusions:
The overall prevalence of depression with T2DM in African Americans is about 25%. The quality of care received by African Americans is lower when compared with non-Hispanic Whites, resulting in more emergency department visits and fewer physician visits per year. African Americans require supportive and trusting collaboration with providers for the ongoing optimal management of these complex conditions.

Implication for practice:
Culturally relevant education on lifestyle modification may help mitigate barriers to management of T2DM and depression in the African American population. Using the diabetes self-management education/support to help empower African Americans may be essential for effective self-management strategies of T2DM and depression.
Partial Epithelial–Mesenchymal Transition Was Observed Under p63 Expression in Acquired Middle Ear Cholesteatoma and Congenital Cholesteatoma
Authors: Takahashi Masahiro , Yamamoto-Fukuda Tomomi , Akiyama Naotaro , Motegi Masaomi , Yamamoto Kazuhisa , Tanaka Yasuhiro , Yamamoto Yutaka , Kojima Hiromi 
Published: July 2019

Introduction:Partial epithelial–mesenchymal transition (p-EMT) is a process by which epithelial cells partially lose their intercellular adhesion and change to obtain migration ability. The transcription factor p63 regulates the expression of cadherin family and induces epithelial cell proliferation. In this study, we hypothesized that p-EMT under p63 expression may be a key factor in epithelial cell growth in middle ear cholesteatoma.Methods:Specimens were surgically excised from patients with congenital cholesteatoma (CC) (n = 48), acquired middle ear cholesteatoma (AC) (n = 120), and normal skin tissue (n = 34). We analyzed immunohistochemically for the EMT marker (N-cadherin), adherence junction marker (E-cadherin), and tight junction marker (claudin-1, claudin-4, occludin). We also examined the labeling index (LI) of p63 and Proliferating cell nuclear antigen (PCNA) (late S phase marker), and Snail expression as a mobility marker.Results:The expression of p63 (CC 51.0 ± 7.4%, AC 50.0 ± 5.9%) was significantly higher in the thickened epithelium of CC and AC compared with normal skin tissue (p < 0.0001). The loss of E-cadherin was observed (CC 50.0%, AC 55.8%) but the expression patterns in the tight junction were almost normal. N-cadherin was partially detected in the basal and upper layer of epithelium in CC and AC. In contrast to that of normal skin tissue, the LI of PCNA was significantly higher in AC (p < 0.0001). The positive rate of Snail was significantly higher in CC (p < 0.0001).Conclusion:This study indicates that p-EMT via the p63 signaling pathway might playsan essential role in epithelial growth in AC and CC formation, although tight junction formation and terminal differentiation were not affected in those processes.
Robotic cholecystectomy for acute cholecystitis
Authors: Milone M. , Vertaldi S. , Bracale U. , D’Ambra M. , Cassese G. , Manigrasso M. , De Palma G.D. 
Published: July 2019

Abstract

Introduction:
Nowadays laparoscopic cholecystectomy is considered as criterion standard for surgical treatment of acute calculous cholecystitis. During the last few years, there has been growing interest about the robotic approach. Several authors have reported the superiority of robotic cholecystectomy, associated with a lower percentage of conversion especially in patients with intraoperative diagnosis of acute or gangrenous cholecystitis. We report 3 case reports of moderate acute cholecystitis successfully treated by robotic cholecystectomy.

Patient Concerns:
Three patients presented moderate acute calculous cholecystitis with leukocytosis, fever, nausea, vomiting, and pain.

Diagnosis:
Three patients of our study population had clinical and laboratory suspicion of moderate acute calculous cholecystitis verified by abdominal ultrasound examination, which found out cholelitiasis in all 3 cases. Final diagnosis was confirmed by intraoperative findings and histopathological examination, with two empyematous cholecystitis and one perforated cholecystitis.

Interventions:
All patients underwent robotic cholecystectomy with the da Vinci Robotic Surgical System. The entire procedure required a mean operation time of 128 minutes and the average blood loss was 60 mL, without any intraoperative complications.

Outcomes:
In all 3 cases postoperative period was uneventfull. All the patients were discharged within 24 hours and no readmissions were reported during a 30 days’ follow-up.

Conclusions:
Robotic cholecystectomy for ACC is feasible and safe. Several studies have demonstrated that robotic approach reduces the risk of conversion to open surgery in case of acute or gangrenous cholecystitis. Our results are in line with current literature. In fact, we have successfully treated 2 patients with empyematous acute cholecystitis and 1 with gangrenous cholecystitis with a totally robotic approach, without any complications or need of conversion to open surgery. In conclusion, our results confirm that it is the time to include robotic surgery in the emergency setting.
Can the prognosis of mantle cell lymphoma be predicted by simple CBC counts?
Authors: Haydaroglu Sahin Handan 
Published: July 2019

Abstract
Mantle cell lymphoma (MCL) exhibits a heterogenous clinical course. The MCL International Prognostic Index (MIPI) is the most commonly used risk classification system in MCL. However, it does not contain a parameter associated with the tumor microenvironment. The aim of this study was to develop a more powerful prognostic index by evaluating the absolute monocyte count (AMC), neutrophil/lymphocyte ratio (NLR), and platelet/lymphocyte ratio (PLR) at diagnosis in conjunction with the clinical and laboratory parameters.
The data of 96 MCL patients with newly diagnosed from January 2014 to December 2018 were retrospectively evaluated in this study. The AMC, NLR, and PLR cut-off values were determined using the receiver operating characteristic (ROC) analysis.
The clinical behavior and results of the disease exhibited significant variation in high and low value groups at the time of diagnosis. In univariate analysis, the AMC ≥ 580, NLR ≥ 2.43, and PLR ≥ 120.85 were determined as negative prognostic factors for 5-year progression free survival (PFS) (AMC: PFS, P < .001; NLR: PFS, P < .001; PLR: PFS, P < .001) and for 5-year overall survival (OS) (P < .001, P < .001, P < .001, respectively). Beta-2 microglobulin (B2-MG), and MIPI for PFS, and for OS were found to be independent risk factors in the multivariate analysis (for PFS: P = .006, P = .002, respectively; and for OS: P = .007, P = .001, respectively). The 5-year OS was 20% in the group with B2-MG ≥ 3.5. The patients in high-risk MIPI group had poorer 5-year OS (median OS: 40 months, P < .001).
The results stated that the use of B2-MG in conjunction with MIPI was a more sensitive method in determining the prognosis in MCL (median OS: 12 months in high-risk MIPI group with a B2-MG ≥3.5, P < .001). Additionally, it was found that parameters reflecting the tumor microenvironment such as AMC, NLR, and PLR increased the risk of progression in MCL. In view of these findings, in addition B2-MG to the MIPI to create a more sensitive prognostic scoring system may provide an insight into personalization of treatment with early recognition of patients with poor prognosis.
Impact of anesthesia management during cytoreductive surgery plus hyperthermic intraperitoneal chemotherapy for the treatment of colorectal peritoneal carcinomatosis on intra- and postoperative outcomes
Authors: Idrissi Moulay , Espitalier Fabien , Coveney Richard , Bélanger Marie-Eve , Gobert Quentin , Sidéris Lucas , Dubé Pierre , Verdonck Olivier , Fortier Louis-Philippe , Richebé Philippe 
Published: July 2019

Abstract

Background:
The impact of the anesthesia management during cytoreductive surgery (CRS) plus hyperthermic intraperitoneal chemotherapy (HIPEC) for the treatment of colorectal peritoneal carcinomatosis (CRPC) on postoperative outcomes might be of major importance in the process of postoperative recovery. It might have a significant impact on intra- and postoperative outcomes, but the evaluation of this impact seems to be under-reported. To investigate the question whether the anesthesia management was reported in previous studies done in this population and if it had any impact on postoperative outcomes, we propose to conduct a systematic review of the published literature.

Methods:
For this review, we will follow the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols (PRISMA-P). Medline/PubMed, Embase, EBM Reviews and the Cochrane Database of Systematic Reviews (CDSR) will be systematically consulted for eligible studies without age, gender, ethnic, and language restriction. The goal of this review will be to assess whether anesthesia monitoring, dosing, and analgesia protocols were reported in this literature on this specific procedure and whether the impact of the anesthesia management on intraoperative safety and postoperative recovery was evaluated.

Results:
The results of this systematic review will allow to answer the initial question: has the impact of anesthesia management on intraoperative safety and patients’ postoperative recovery already been studied and reported in the past for this type of major surgery? And does anesthesia have any impact on postoperative outcomes?

Discussion:
In the hypothesis that the impact of anesthesia management on patients’ postoperative recovery has never been studied, or very little reported in previous studies in this type of major surgery, it would be justified to conduct a randomized controlled trial on this specific objective.

Registration:
This systematic review protocol was registered in PROSPERO, under the registration number CRD42019124162.
Efficacy of laser acupuncture for carpal tunnel syndrome
Authors: Chen Chuan-Chih , Wu Yung-Tsan , Su Yu-Chi , Shen Yu-Ping , Chen Fang-Pey 
Published: July 2019

Abstract

Introduction:
Carpal tunnel syndrome (CTS) is the most common entrapment neuropathy that causes hand discomfort and work disability. Since no satisfactory conventional treatments for mild to moderate CTS exist, we apply complementary alternative medicine (CAM) to this problem. Laser acupuncture (LA), a new, non-invasive therapy which uses low-level-laser therapy (LLLT) in acupuncture could help to manage CTS. However, only one small randomized, double-blind and crossover trial had been conducted, which is not enough to provide an evidence-based assessment of the effects of LA on CTS.

Objectives:
The aim of this study protocol is to investigate the efficacy of LA therapy on patients with mild to moderate CTS through sonography of the median nerve and offer clear parameters of LLLT.

Methods:
This study protocol is a prospective double-blind randomized controlled trial. Forty subjects aged 20 to 80 years old and diagnosed as having mild to moderate CTS will be randomly assigned to the intervention group (real LA, 3-sessions a week for 2 weeks) and control group (sham LA, 3-sessions a week for 2 weeks). All subjects will be asked to wear night splints as the fundamental management approach. The laser parameters will include a wavelength of 808 nm, power output of 300 mW and power density of 300 mW/mm2, with ten seconds of treatment for each acupuncture point (PC4, PC6, PC7, PC8, LI4, LI10, LI11, HT3, HT7, and LU10). Sham LA treatment will be applied without any laser power output. The primary outcome will be based the Boston Carpal Tunnel Syndrome Questionnaire and secondary outcomes included a visual analog scale, cross sectional area of median nerve by sonography and electrophysiological test before interventions and after 2, 4, 8, 12 weeks postintervention.

Trial registration:
ClinicalTrials.gov (Identifier: NCT03580265).