bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nutritional Contribution of Biofloc within the Diet of Growout and Broodstock of Litopenaeus vannamei, Determined by Stable Isotopes and Fatty Acids
Authors: Magaña‐Gallegos Edén , González‐Zúñiga Rodrigo , Cuzon Gerard , Arevalo Miguel , Pacheco Eduardo , Valenzuela Manuel A. J. , Gaxiola Gabriela , Chan‐Vivas Elisa , López‐Aguiar Korinthia , Noreña‐Barroso Elsa 
Published: February 2018

AbstractThe relative contributions of feed sources were determined through the isotopic signal (δ13C and δ15N) and fatty acid profile of feed items, shrimp muscle, and eggs of Litopenaeus vannamei reared in a biofloc system. In the growout phase, the isotope analysis showed the biofloc particle size class ≥250 μm contributed 55–100%; size ≥50 < 250 μm contributed 0–22%; and artificial feed contributed 0–45%. Principal component analysis applied to fatty acid profiles showed that biofloc ≥250 μm and artificial feed were the most important items in shrimp growout. For the egg production, isotope analysis suggested that the most important feed sources according to their relative contributions were polychaetes (0–100%), followed by artemia biomass (0–86%) and semi‐moist feed (0–66%), with lower contributions from squid, mussel, and the muscle of L. vannamei broodstock that had been cultured in biofloc. In terms of fatty acids, the most important items were artemia, polychaetes, and semi‐moist feeds. This work clarified the importance of feed sources for shrimp during culture in biofloc systems and during reproduction. Analysis of stable isotopes and fatty acids can be successfully used to trace the assimilation of nutrients during the nutrition of shrimp.
The miR‐139‐5p regulates proliferation of supratentorial paediatric low‐grade gliomas by targeting the PI3K/AKT/mTORC1 signalling
Authors: Catanzaro G. , Besharat Z. M. , Miele E. , Chiacchiarini M. , Po A. , Carai A. , Marras C. E. , Antonelli M. , Badiali M. , Raso A. , Mascelli S. , Schrimpf D. , Stichel D. , Tartaglia M. , Capper D. , von Deimling A. , Giangaspero F. , Mastronuzzi A. , Locatelli F. , Ferretti E. 
Published: February 2018

AimsPaediatric low‐grade gliomas (pLGGs) are a heterogeneous group of brain tumours associated with a high overall survival: however, they are prone to recur and supratentorial lesions are difficult to resect, being associated with high percentage of disease recurrence. Our aim was to shed light on the biology of pLGGs.MethodsWe performed microRNA profiling on 45 fresh‐frozen grade I tumour samples of various histological classes, resected from patients aged ≤16 years. We identified 93 microRNAs specifically dysregulated in tumours as compared to non‐neoplastic brain tissue. Pathway analysis of the microRNAs signature revealed PI3K/AKT signalling as one of the centrally enriched oncogenic signalling. To date, activation of the PI3K/AKT pathway in pLGGs has been reported, although activation mechanisms have not been fully investigated yet.ResultsOne of the most markedly down‐regulated microRNAs in our supratentorial pLGGs cohort was miR‐139‐5p, whose targets include the gene encoding the PI3K's (phosphatidylinositol 3‐kinase) catalytic unit, PIK3CA. We investigated the role of miR‐139‐5p in regulating PI3K/AKT signalling by the use of human cell cultures derived from supratentorial pLGGs. MiR‐139‐5p overexpression inhibited pLGG cell proliferation and decreased the phosphorylation of PI3K target AKT and phosphorylated‐p70 S6 kinase (p‐p70 S6K), a hallmark of PI3K/AKT/mTORC1 signalling activation. The effect of miR‐139‐5p was mediated by PI3K inhibition, as suggested by the decrease in proliferation and phosphorylation of AKT and p70 S6K after treatment with the direct PI3K inhibitor LY294002.ConclusionsThese findings provide the first evidence that down‐regulation of miR‐139‐5p in supratentorial pLGG drives cell proliferation by derepressing PI3K/AKT signalling.
Relationship tensions and mood: Adult children's daily experience of aging parents' stubbornness
Authors: HEID ALLISON R. , KIM KYUNGMIN , ZARIT STEVEN H. , BIRDITT KIRA S. , FINGERMAN KAREN L. 
Published: February 2018

AbstractThis study examined middle‐aged individuals' reports of parents' behaviors commonly attributed to stubbornness. Middle‐aged adults (N = 192) completed a 7‐day diary reporting their mood and how often they felt their parents (N = 254) engaged in behaviors often described as “stubbornness” (insistent or risky). Thirty‐one percent of middle‐aged children reported insistent behaviors, and 17% reported risky behaviors by their parent(s). Daily reports of parent behaviors attributed to stubbornness were positively associated with parent–child relationship quality, parent functional limitations, and child neuroticism. Reports of perceived parent insistent behaviors were also associated with greater daily negative mood among adult children. Findings highlight the impact of adult children's daily perceptions of parent behaviors commonly attributed to stubbornness on the individual and relationship.
Parasitized Natural Killer cells do not facilitate the spread of Toxoplasma gondii to the brain
Authors: Petit‐Jentreau L. , Glover C. , Coombes J. L. 
Published: February 2018

SummaryToxoplasma gondii is a protozoan parasite capable of invading immune cells and co‐opting their migratory pathways to disseminate through the host. Natural Killer (NK) cells can be directly invaded by the parasite and this invasion alters NK cell migration, producing a hypermotile phenotype. However, the consequences of this hypermotile phenotype for the dissemination of T. gondii to the brain remain unknown. To address this, C57BL6/J mice were infected with freshly egressed tachyzoites (type IIPrugniaud strain) or with parasitized NK cells. Under both conditions, parasite loads in the brain were comparable, indicating that parasitized NK cells were not able to facilitate spread of T. gondii to the brain. Consistent with this, we found no evidence for the recruitment of endogenous NK cells to the brain at early time points post‐infection, nor any changes in the expression of α4β1 integrin, involved in recruitment of NK cells to the brain. We therefore found no evidence for a role for hypermotile NK cells in delivery of parasites to the brain during acute infection with T. gondii.
Vertical ridge augmentation in the esthetic zone
Authors: Rocchietta Isabella , Ferrantino Luca , Simion Massimo 
Published: February 2018

AbstractThe reconstruction of deficient alveolar ridges using vertical and/or horizontal guided bone regeneration techniques allows for ideal implant placement, which is crucial for function and also for esthetically successful outcomes. Unlike in the past, when meeting a patient's functional demands was sufficient, many patients now have greater expectations from their implant restoration. Hence, it is no longer enough simply to restore the edentulous space with a functioning tooth or teeth. It has been suggested that patients now measure their final restoration using the contralateral natural tooth as the gold standard. Both subjective and objective levels of patient information on dental implants have increased significantly in the last decade. As a result of this demand, implant literature has inherited and developed specific esthetic parameters and patient‐centered outcomes from studies in the restorative field. Unfortunately, studies reporting on guided bone regeneration in the esthetic zone entirely lack such parameters and outcomes. Currently, there is a strong need for a consensus on objective and well‐defined parameters to assess the esthetics in bone regeneration and subsequently on implant dentistry.
Horizontal bone‐augmentation procedures in implant dentistry: prosthetically guided regeneration
Authors: Chiapasco Matteo , Casentini Paolo 
Published: February 2018

AbstractThe rehabilitation of partially or totally edentulous patients with implant‐supported prostheses has become routine, with excellent long‐term outcome. A proper implant position is mandatory to achieve good functional and esthetic outcome and may require an adequate amount of alveolar bone and surrounding soft tissue. When this is lacking because of atrophy, sequelae of periodontal disease, traumas or congenital malformations, increased bone volume and/or keratinized mucosa can be obtained by guided bone regeneration, bone‐grafting techniques and alveolar bone expansion. This article presents an evidence‐based, prosthetically driven approach for the treatment of edentulous ridges with horizontal defects. The classification of bony defects, the main augmentation techniques, the selection criteria among different surgical procedures for different types of bony defects, and the advantages, disadvantages and limitations of each technique, are described in detail.
Reviewer Acknowledgements
Authors: Willianms Ellery 
Published: February 2018

Business and Management Studies (BMS) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether BMS publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue.Reviewers for Volume 4, Number 1 Abdul-Kahar Adam, University of Education, Winneba, GhanaAndrzej Niemiec, Poznań University of Economics and Business, PolandAsad Ghalib, The University of Manchester, UKAshford Chea, Benedict College, USAComite Ubaldo, University of Calabria, ItalyDaiane Miranda Freitas, FACISA/Univicosa, BrazilDalia Susniene, Kaunas University of Technology, LithuaniaFlorin Peci, University of Peja, KosovoGabriela O. Chiciudean, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, RomaniaJulia Stefanova, Bulgarian Academy of Sciences, BulgariaKonstantinos N. Malagas, University of the Aegean, GreeceLucie Andreisová, University of Economics in Prague, CzechMike Rayner, University of Portsmouth, UKMythili Kolluru, College of Banking and Financial Studies, OmanOleksandr Mosin, National Mining University, UkraineOlha Komelina, Yuri Kondratyuk University, UkraineRashedul Hasan, International Islamic University Malaysia, MalaysiaRegina Lenart-Gansiniec, Jagiellonian University, PolandRocsana Tonis, Spiru Haret University, RomaniaSammy Kimunguyi, Office of The Auditor-General, KenyaTetiana Paientko, Kyiv National Economic Univercity, UkraineUmair Akram, Beijing Univ Posts & Telecommun, PAKISTANWaeibrorheem Waemustafa, Universiti Utara Malaysia, MalaysiaYanzhe Zhang, University of Canberra, AustraliaZeki Atıl Bulut, Dokuz Eylul University, Turkey Ellery WillianmsEditorial AssistantOn behalf of,The Editorial Board of Business and Management StudiesRedfame Publishing9450 SW Gemini Dr. #99416Beaverton, OR 97008, USAURL: http://bms.redfame.com
Stretch‐induced Ca2+ independent ATP release in hippocampal astrocytes
Authors: Xiong Yingfei , Teng Sasa , Zheng Lianghong , Sun Suhua , Li Jie , Guo Ning , Li Mingli , Wang Li , Zhu Feipeng , Wang Changhe , Rao Zhiren , Zhou Zhuan 
Published: February 2018

Key points
Similar to neurons, astrocytes actively participate in synaptic transmission via releasing gliotransmitters.
The Ca2+‐dependent release of gliotransmitters includes glutamate and ATP.
Following an ‘on‐cell‐like’ mechanical stimulus to a single astrocyte, Ca2+ independent single, large, non‐quantal, ATP release occurs.
Astrocytic ATP release is inhibited by either selective antagonist treatment or genetic knockdown of P2X7 receptor channels.
Our work suggests that ATP can be released from astrocytes via two independent pathways in hippocampal astrocytes; in addition to the known Ca2+‐dependent vesicular release, larger non‐quantal ATP release depends on P2X7 channels following mechanical stretch.
AbstractAstrocytic ATP release is essential for brain functions such as synaptic long‐term potentiation for learning and memory. However, whether and how ATP is released via exocytosis remains hotly debated. All previous studies of non‐vesicular ATP release have used indirect assays. By contrast, two recent studies report vesicular ATP release using more direct assays. In the present study, using patch clamped ‘ATP‐sniffer cells’, we re‐investigated astrocytic ATP release at single‐vesicle resolution in hippocampal astrocytes. Following an ‘on‐cell‐like’ mechanical stimulus of a single astrocyte, a Ca2+ independent single large non‐quantal ATP release occurred, in contrast to the Ca2+‐dependent multiple small quantal ATP release in a chromaffin cell. The mechanical stimulation‐induced ATP release from an astrocyte was inhibited by either exposure to a selective antagonist or genetic knockdown of P2X7 receptor channels. Functional P2X7 channels were expressed in astrocytes in hippocampal brain slices. Thus, in addition to small quantal ATP release, larger non‐quantal ATP release depends on P2X7 channels in astrocytes.
Dynamic Dispersal of Surface Layer Biofilm Induced by Nanosized TiO
2
Based on Surface Plasmon Resonance and Waveguide
Authors: Zhang Peng , Guo Jin-Song , Yan Peng , Chen You-Peng , Wang Wei , Dai You-Zhi , Fang Fang , Wang Gui-Xue , Shen Yu 
Published: February 2018

ABSTRACT

Pollutant degradation is present mainly in the surface layer of biofilms, and the surface layer is the most vulnerable to impairment by toxic pollutants. In this work, the effects of nanosized TiO
2
(n-TiO
2
) on the average thicknesses of
Bacillus subtilis
biofilm and on bacterial attachment on different surfaces were investigated. The binding mechanism of n-TiO
2
to the cell surface was also probed. The results revealed that n-TiO
2
caused biofilm dispersal and the thicknesses decreased by 2.0 to 2.6 μm after several hours of exposure. The attachment abilities of bacteria with extracellular polymeric substances (EPS) on hydrophilic surfaces were significantly reduced by 31% and 81% under 10 and 100 mg/liter of n-TiO
2
, respectively, whereas those of bacteria without EPS were significantly reduced by 43% and 87%, respectively. The attachment abilities of bacteria with and without EPS on hydrophobic surfaces were significantly reduced by 50% and 56%, respectively, under 100 mg/liter of n-TiO
2
. The results demonstrated that biofilm dispersal can be attributed to the changes in the cell surface structure and the reduction of microbial attachment ability.

IMPORTANCE
Nanoparticles can penetrate into the outer layer of biofilm in a relatively short period and can bind onto EPS and bacterial surfaces. The current work probed the effects of nanosized TiO
2
(n-TiO
2
) on biofilm thickness, bacterial migration, and surface properties of the cell in the early stage using the surface plasmon resonance waveguide mode. The results demonstrated that n-TiO
2
decreased the adhesive ability of both cell and EPS and induced bacterial migration and biofilm detachment in several hours. The decreased adhesive ability of microbes and EPS worked against microbial aggregation, reducing the effluent quality in the biological wastewater treatment process.
Complete Genome Sequence of
Streptomyces lavendulae
subsp.
lavendulae
CCM 3239 (Formerly “
Streptomyces aureofaciens
CCM 3239”), a Producer of the Angucycline-Type Antibiotic Auricin
Authors: Busche Tobias , Novakova Renata , Al'Dilaimi Arwa , Homerova Dagmar , Feckova Lubomira , Rezuchova Bronislava , Mingyar Erik , Csolleiova Dominika , Bekeova Carmen , Winkler Anika , Sevcikova Beatrica , Kalinowski Jörn , Kormanec Jan , Rückert Christian 
Published: February 2018

ABSTRACT

Streptomyces lavendulae
subsp.
lavendulae
CCM 3239 produces the angucycline antibiotic auricin and was thought to be the type strain of
Streptomyces aureofaciens
. We report the complete genome sequence of this strain, which consists of a linear chromosome and the linear plasmid pSA3239, and demonstrate it to be
S. lavendulae
subsp.
lavendulae
.
Do female sex workers have lower uptake of HIV treatment services than non-sex workers? A cross-sectional study from east Zimbabwe
Authors: Rhead Rebecca , Elmes Jocelyn , Otobo Eloghene , Nhongo Kundai , Takaruza Albert , White Peter J , Nyamukapa Constance Anesu , Gregson Simon 
Published: February 2018

ObjectiveGlobally, HIV disproportionately affects female sex workers (FSWs) yet HIV treatment coverage is suboptimal. To improve uptake of HIV services by FSWs, it is important to identify potential inequalities in access and use of care and their determinants. Our aim is to investigate HIV treatment cascades for FSWs and non-sex workers (NSWs) in Manicaland province, Zimbabwe, and to examine the socio-demographic characteristics and intermediate determinants that might explain differences in service uptake.MethodsData from a household survey conducted in 2009–2011 and a parallel snowball sample survey of FSWs were matched using probability methods to reduce under-reporting of FSWs. HIV treatment cascades were constructed and compared for FSWs (n=174) and NSWs (n=2555). Determinants of service uptake were identified a priori in a theoretical framework and tested using logistic regression.ResultsHIV prevalence was higher in FSWs than in NSWs (52.6% vs 19.8%; age-adjusted OR (AOR) 4.0; 95% CI 2.9 to 5.5). In HIV-positive women, FSWs were more likely to have been diagnosed (58.2% vs 42.6%; AOR 1.62; 1.02–2.59) and HIV-diagnosed FSWs were more likely to initiate ART (84.9% vs 64.0%; AOR 2.33; 1.03–5.28). No difference was found for antiretroviral treatment (ART) adherence (91.1% vs 90.5%; P=0.9). FSWs’ greater uptake of HIV treatment services became non-significant after adjusting for intermediate factors including HIV knowledge and risk perception, travel time to services, physical and mental health, and recent pregnancy.ConclusionFSWs are more likely to take up testing and treatment services and were closer to achieving optimal outcomes along the cascade compared with NSWs. However, ART coverage was low in all women at the time of the survey. FSWs’ need for, knowledge of and proximity to HIV testing and treatment facilities appear to increase uptake.
I did NOT feel like this at all before the accident: do men and women report different health and life consequences of a road traffic injury?
Authors: Hasselberg Marie , Kirsebom Marie , Bäckström Josefin , Berg Hans-Yngve , Rissanen Ritva 
Published: February 2018

BackgroundWorldwide, injuries represent one of the leading causes of mortality, and nearly one-quarter of all injuries are road traffic related. In many high-income countries, the burden of road traffic injuries (RTIs) has shifted from premature death to injury and disability with long-term consequences; therefore, it is important to assess the full burden of an RTI on individual lives.ObjectiveTo describe how men and women with minor and moderate injuries reported the consequences of an RTI on their health and lives.MethodsThe study was designed as an explorative qualitative study, in which the answers to an open-ended question concerning the life and health consequences following injury were analysed using systematic text condensation.ParticipantsA total of 692 respondents with a minor or a moderate injury were included.ResultsThe respondents reported the consequences of the crash on their health and lives according to four categories: physical consequences, psychological consequences, everyday life consequences and financial consequences. The results show that medically classified minor and moderate injuries have detrimental long-term health and life consequences. Although men and women report some similar consequences, there are substantial differences in their reported psychological and everyday life consequences following an injury. Women report travel anxiety and PTSD-like symptoms, being life altering for them compared with men, for whom these types of reports were missing.ConclusionThese differences emphasise the importance of considering gender-specific physical and psychological consequences following an RTI.
Effect of ageing on the calibration of ballistic gelatin
Authors: Guey Jason , Rodrigues S , Pullen A , Shaw B , Kieser D C 
Published: February 2018

PurposeBallistic gelatin is commonly used as a validated surrogate for soft tissue during terminal ballistic testing. However, the effect of a delay between production and testing of a gelatin mould remains unknown. The aim of this study was to determine any potential effects of ageing on ballistic gelatin.MethodsDepth of penetration (DoP) of 4.5 mm spherical fragment simulating projectiles was ascertained using mixtures of 10%, 11.25% and 20% Type A 250 Bloom ballistic gelatin. Testing was performed daily for 5 days using velocities between 75 and 210 m/s. DoP at day 5 was statistically compared with day 1, and net mass change was recorded daily.ResultsNo significant difference was found for DoP observed with time in any of the samples (P>0.05). Spearman correlation was excellent in all moulds. The moulds with known standard calibrations remained in calibration throughout the study period. Mass loss of less than 1% was noted in all samples.ConclusionMass loss was the only quantifiable measure of changes in the blocks with time, but did not correlate with any changes in DoP. This may provide reassurance when undertaking such testing that an inadvertent delay will not significantly alter the penetration properties of the mould. Future research is recommended to determine any potential effect on the mechanical properties of gelatin at higher velocity impacts and whether the calibration corresponds to an adequate simulation under such conditions.
Treatment of blood blister aneurysms of the internal carotid artery with flow diversion
Authors: Mokin Maxim , Chinea Angel , Primiani Christopher T , Ren Zeguang , Kan Peter , Srinivasan Visish M , Hanel Ricardo , Aguilar-Salinas Pedro , Turk Aquilla S , Turner Raymond D , Chaudry M Imran , Ringer Andrew J , Welch Babu G , Mendes Pereira Vitor , Renieri Leonardo , Piano Mariangela , Elijovich Lucas , Arthur Adam S , Cheema Ahmed , Lopes Demetrius Klee , Saied Ahmed , Baxter Blaise W , Hawk Harris , Puri Ajit S , Wakhloo Ajay K , Shallwani Hussain , Levy Elad I , Siddiqui Adnan H , Dabus Guilherme , Linfante Italo 
Published: February 2018

BackgroundBlood blister aneurysms (BBA) are a rare subset of intracranial aneurysms that represent a therapeutic challenge from both a surgical and endovascular perspective.ObjectiveTo report multicenter experience with flow diversion exclusively for BBA, located at non-branching segments along the anteromedial wall of the supraclinoidal internal carotid artery (ICA).MethodsConsecutive cases of BBA located at non-branching segments along the anteromedial wall of the supraclinoidal ICA treated with flow diversion were included in the final analysis.Results49 patients with 51 BBA of the ICA treated with devices to achieve the flow diversion effect were identified. 43 patients with 45 BBA of the ICA were treated with the pipeline embolization device and were included in the final analysis. Angiographic follow-up data were available for 30 patients (32 aneurysms in total); 87.5% of aneurysms (28/32) showed complete obliteration, 9.4% (3/32) showed reduced filling, and 3.1% (1/32) persistent filling. There was no difference between the size of aneurysm (≤2 mm vs >2 mm) or the use of adjunct coiling and complete occlusion of the aneurysm on follow-up (P=0.354 and P=0.865, respectively). Clinical follow-up data were available for 38 of 43 patients. 68% of patients (26/38) had a good clinical outcome (modified Rankin scale score of 0–2) at 3 months. There were 7 (16%) immediate procedural and 2 (5%) delayed complications, with 1 case of fatal delayed re-rupture after the initial treatment.ConclusionsOur data support the use of a flow diversion technique as a safe and effective therapeutic modality for BBA of the supraclinoid ICA.