bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sound Frequency Representation in the Auditory Cortex of the Common Marmoset Visualized Using Optical Intrinsic Signal Imaging
Authors: Tani Toshiki , Abe Hiroshi , Hayami Taku , Banno Taku , Miyakawa Naohisa , Kitamura Naohito , Mashiko Hiromi , Ichinohe Noritaka , Suzuki Wataru 
Published: April 2018

AbstractNatural sound is composed of various frequencies. Although the core region of the primate auditory cortex has functionally defined sound frequency preference maps, how the map is organized in the auditory areas of the belt and parabelt regions is not well known. In this study, we investigated the functional organizations of the core, belt, and parabelt regions encompassed by the lateral sulcus and the superior temporal sulcus in the common marmoset (Callithrix jacchus). Using optical intrinsic signal imaging, we obtained evoked responses to band-pass noise stimuli in a range of sound frequencies (0.5–16 kHz) in anesthetized adult animals and visualized the preferred sound frequency map on the cortical surface. We characterized the functionally defined organization using histologically defined brain areas in the same animals. We found tonotopic representation of a set of sound frequencies (low to high) within the primary (A1), rostral (R), and rostrotemporal (RT) areas of the core region. In the belt region, the tonotopic representation existed only in the mediolateral (ML) area. This representation was symmetric with that found in A1 along the border between areas A1 and ML. The functional structure was not very clear in the anterolateral (AL) area. Low frequencies were mainly preferred in the rostrotemplatal (RTL) area, while high frequencies were preferred in the caudolateral (CL) area. There was a portion of the parabelt region that strongly responded to higher sound frequencies (>5.8 kHz) along the border between the rostral parabelt (RPB) and caudal parabelt (CPB) regions.
Dynamic Encoding of Incentive Salience in the Ventral Pallidum: Dependence on the Form of the Reward Cue
Authors: Ahrens Allison M. , Ferguson Lindsay M. , Robinson Terry E. , Aldridge J. Wayne 
Published: April 2018

AbstractSome rats are especially prone to attribute incentive salience to a cue (conditioned stimulus, CS) paired with food reward (sign-trackers, STs), but the extent they do so varies as a function of the form of the CS. Other rats respond primarily to the predictive value of a cue (goal-trackers, GTs), regardless of its form. Sign-tracking is associated with greater cue-induced activation of mesolimbic structures than goal-tracking; however, it is unclear how the form of the CS itself influences activity in neural systems involved in incentive salience attribution. Thus, our goal was to determine how different cue modalities affect neural activity in the ventral pallidum (VP), which is known to encode incentive salience attribution, as rats performed a two-CS Pavlovian conditioned approach task in which both a lever-CS and a tone-CS predicted identical food reward. The lever-CS elicited sign-tracking in some rats (STs) and goal-tracking in others (GTs), whereas the tone-CS elicited only goal-tracking in all rats. The lever-CS elicited robust changes in neural activity (sustained tonic increases or decreases in firing) throughout the VP in STs, relative to GTs. These changes were not seen when STs were exposed to the tone-CS, and in GTs there were no differences in firing between the lever-CS and tone-CS. We conclude that neural activity throughout the VP encodes incentive signals and is especially responsive when a cue is of a form that promotes the attribution of incentive salience to it, especially in predisposed individuals.
Mortality After Pediatric Arterial Ischemic Stroke
Authors: Beslow Lauren A. , Dowling Michael M. , Hassanein Sahar M.A. , Lynch John K. , Zafeiriou Dimitrios , Sun Lisa R. , Kopyta Ilona , Titomanlio Luigi , Kolk Anneli , Chan Anthony , Biller Jose , Grabowski Eric F. , Abdalla Abdalla A. , Mackay Mark T. , deVeber Gabrielle ,   on behalf of the International Pediatric Stroke Study Investigators
Published: April 2018

OBJECTIVES:
Cerebrovascular disease is among the top 10 causes of death in US children, but risk factors for mortality are poorly understood. Within an international registry, we identify predictors of in-hospital mortality after pediatric arterial ischemic stroke (AIS).

METHODS:
Neonates (0–28 days) and children (29 days–&lt;19 years) with AIS were enrolled from January 2003 to July 2014 in a multinational stroke registry. Death during hospitalization and cause of death were ascertained from medical records. Logistic regression was used to analyze associations between risk factors and in-hospital mortality.

RESULTS:
Fourteen of 915 neonates (1.5%) and 70 of 2273 children (3.1%) died during hospitalization. Of 48 cases with reported causes of death, 31 (64.6%) were stroke-related, with remaining deaths attributed to medical disease. In multivariable analysis, congenital heart disease (odds ratio [OR]: 3.88; 95% confidence interval [CI]: 1.23–12.29; P = .021), posterior plus anterior circulation stroke (OR: 5.36; 95% CI: 1.70–16.85; P = .004), and stroke presentation without seizures (OR: 3.95; 95% CI: 1.26–12.37; P = .019) were associated with in-hospital mortality for neonates. Hispanic ethnicity (OR: 3.12; 95% CI: 1.56–6.24; P = .001), congenital heart disease (OR: 3.14; 95% CI: 1.75–5.61; P &lt; .001), and posterior plus anterior circulation stroke (OR: 2.71; 95% CI: 1.40–5.25; P = .003) were associated with in-hospital mortality for children.

CONCLUSIONS:
In-hospital mortality occurred in 2.6% of pediatric AIS cases. Most deaths were attributable to stroke. Risk factors for in-hospital mortality included congenital heart disease and posterior plus anterior circulation stroke. Presentation without seizures and Hispanic ethnicity were also associated with mortality for neonates and children, respectively. Awareness and study of risk factors for mortality represent opportunities to increase survival.
Three-dimensional structure of the complex between calmodulin mutant lacking the c-terminal five residues and the calmodulin-binding peptide derived from skeletal muscle myosin light-chain kinase
Authors: Dung Vu Van , Umetsu Yoshitaka , Ohki Shinya 
Published: April 2018

In our previous study, functional ability and conformational stability had been examined for C-terminal deletion mutants of a 148-residue Ca2+-binding protein, chicken calmodulin (CaM). In that study, we had reported that a mutant named CCMΔ5, missing five residues at the C-terminus, activates CaM-target as much as full-length CaM does. This finding is intriguing because CCMΔ5 lacks the key residues, Met144 and Met145, for the target activation. To uncover why CCMΔ5 displays proper function, here we report the three-dimensional structure of CCMΔ5 bound to the peptide derived from skeletal muscle myosin light-chain kinase (skMLCK). The structure determination was achieved using multidimensional nuclear magnetic resonance (NMR) spectroscopy. The complex structure of CCM∆5-skMLCK was compared to that of wild CaM-skMLCK. The results showed that the orientation of helix-1 and helix-5 in CCM∆5 differs from those in wild CaM. Moreover, distinctive hydrophobic interaction manner was found in the binding between CCM∆5 and peptide; Phe141, Ala128, Met109, Leu105 and Phe92 of CCM∆5 contribute to the interaction with Trp4 of the skMLCK peptide. Abbreviations: CaM, calmodulin; CCMΔX, a deletion mutant of CaM that lacks X C-terminal residues; NMR, Nuclear magnetic resonance; PDB, Protein date bank; skMLCK, skeletal muscle myosin light-chain kinase; TOF-MS, Time-of-flight mass spectrometry; RMSD, root mean square deviation; SDS-PAGE, Sodium dodecyl sulfate polyacrylamide gel electrophoresis Citation: Vu Van Dung, Umetsu Y., Ohki S., 2017. Three-dimensional structure of the complex between calmodulin mutant lacking the c-terminal five residues and the calmodulin-binding peptide derived from skeletal muscle myosin light-chain kinase. Tap chi Sinh hoc, 39(3): 309-319. DOI: 10.15625/0866-7160/v39n3.10111. *Corresponding author: vandungbio46@yahoo.com Received 19 June 2017, accepted 20 August 2017
Study on biocharacteristics of Symbiodinium sp. isolated from soft coral in coastal water of Quang Nam, south central Vietnam
Authors: Hong Dang Diem , Nhat Pham Van , Huong Quynh Hoang Thi , Tam Luu Thi , Hoai Thu Ngo Thi , Ha Nguyen Cam , Lan Anh Hoang Thi , Nam Nguyen Hoai , Minh Hang Nguyen Thi , Minh Chau Van 
Published: April 2018

Phototrophic dinoflagellates of the genus Symbiodinium (= zooxanthellae) are well known as symbionts of corals. They play an important role in maintaining the healthy as well as establishment of new reefs. In addition, products such as toxins or pigments from these symbiotic microalgae are potentially important for applications in the biomedical sciences, especially in new drug development. In this study, we isolated successfully a symbiotic microalga from soft coral Sinularia sp. which was collected in Cu Lao Cham coastal waters of Quang Nam province. Based on morphological characteristics and 18S rRNA gene sequences, isolated SHM8 strain was identified as Symbiodinium goreaui. This symbiont was cultured in different media such as ASP-A8, F2, ES, IMK, K, and L1. L1 culture medium supplemented with taurine concentration of 4.88 mM led to the cell density of SHM8 strain increased in 50% compared to control on day 2 in cultivation; amino acid mixture enhanced cell density by 31.7% on day 5 in cultivation. Supplementation of carbon source (bicarbonate salt of 16.8 g/L) and nitrogen source (nitrate salt of 0.055 g/L) enhanced the cell density by 40% and 27%, respectively on day 6 in culture. Obtained results in this study proved that this microalga can live independently without host. Successful isolation and cultivation of Symbiodinium microalga are one of the necessary conditions for promising researches on the natural bioactive substances from this microalgal biomass in the future in Vietnam. Citation: Dang Diem Hong, Pham Van Nhat, Hoang Thi Huong Quynh, Luu Thi Tam, Ngo Thi Hoai Thu, Nguyen Cam Ha, Hoang Thi Lan Anh, Nguyen Hoai Nam, Nguyen Thi Minh Hang, Chau Van Minh, 2017. Study on biocharacteristics of Symbiodinium sp. isolated from soft coral in coastal water of Quang Nam, south central Vietnam. Tap chi Sinh hoc, 39(3): 367-375. DOI: 10.15625/0866-7160/v39n3.10112. *Corresponding author: ddhong60vn@yahoo.com Received 19 June 2017, accepted 20 August 2017
Establishment of a procedure for producing the anti-b monoclonal antibody from B4C10D9 hybridoma cell line
Authors: Trung Nguyen Thi , Hai Truong Nam 
Published: April 2018

There are two the most important blood group system in blood transfusion, that is the ABO system and the Rh system. The anti-A or anti-B antibodies in the blood of recipient cause strongly agglutination the red blood cells of donor bearing A or B antigens on their surface respectively. So, all donated blood and received blood must be typed group for the ABO system to be safe in blood transfusion. To blood grouping, used of know antisera reacts with the whole blood samples to determine antigen on the surface of red blood cell or use of know red blood cells reacts with the individual's serum to determine antibody. The serology method is widely used, antisera are usually monoclonal antibodies secreted by hybridoma cells. Our previous studies showed that the B4C10D9 hybridoma cell line generated anti-B monoclonal antibody was produced by hybridoma technology. This antibody causes specificity agglutination the red blood cells caring B antigen on their surface. It is classed as IgM antibody with it heavy chains are µ and κ light chains. The aim of the study was to produce the anti-B monoclonal antibody form B4C10D9 hybridoma cells line serve as a part of ABO blood grouping reagent. Based on results of experiments, procedure for producing the anti-B monoclonal antibody from B4C10D9 hybridoma cell line has also been established with following steps. At first, refresh the frozen B4C10D9 hybridoma cells and the density of B4C10D9 hybridoma cells is 4.0 x 104 cells/ml at 24 hours post-inoculation. Then the B4C10D9 hybridoma cells are splitted 1:5 every 48 hours for 2 times to obtain the cell biomass. In the next step, the hybridoma cells are splitted into 125 cm2 flasks with the initial cell density of 105 cells/ml. The B4C10D9 hybridoma cells density reach 9.9 x 106 cells/ml at 48 hours post-inoculation. After 144 hours of post-inoculation, collect culture medium and concentrate the anti-B monoclonal antibody by precipitation with saturated ammonium sulfate at a final concentration of 50%. If resuspending volume equals initial volume, then the anti-B monoclonal antibody titer reaches 1/256 and the antibody reaction intensity will be 3+. And if the obtained antibody solution volume equals one fifth of the original volume, then the anti-B monoclonal antibody titer reaches 1/1024, and the antibody reaction intensity will be 4+. Citation: Establishment of a procedure for producing the anti-b monoclonal antibody from B4C10D9 hybridoma cell line. Tap chi Sinh hoc, 39(3): 342-348. DOI: 10.15625/0866-7160/v39n3.10765. *Corresponding author: tnhai@ibt.ac.vn Received 20 August 2017, accepted 20 September 2017
Life history of Chrysozephyrus alienus (Koiwaya & Monastyrskii, 2010) (Lepidoptera) in central Vietnam
Authors: Hasegawa Takashi , Saito Kotaro , Lien Vu Van , Miyagawa Takashi , Kubota Akiko , Yokochi Takashi 
Published: April 2018

We have successfully reared the larvae of Chrysozephyrus alienus (Koiwaya & Monastyrskii, 2010), and presented the life history of this species. The larval foodplant was found to be Lyonia ovalifolia var. pubescens. The tactile setae of the 1st instar larvae are absent at dorsal anterior area at the 1st abdominal segment and dorsal area at the 7th abdominal segment. The pattern is rather similar to that of Chrysozephyrus scintillans. Citation: Hasegawa T., Saito K., Vu Van Lien, Miyagawa T., Kubota A., Yokochi T., 2017. Life history of Chrysozephyrus alienus (Koiwaya & Monastyrskii, 2010) (Lepidoptera) in central Vietnam. Tap chi Sinh hoc, 39(3): 291-295. DOI: 10.15625/0866-7160/v39n3.9348. *Corresponding author: airsjapan64@gmail.com Received 20 March 2017, accepted 20 August 2017
Biodegradation of aniline by freely suspended and immobilized Pseudomonas moraviensis AN-5
Authors: Duc Ha Danh , Triet Bui Minh 
Published: April 2018

Pseudomonas moraviensis AN-5 isolated from contaminated sludge could utilize aniline as a sole carbon and nitrogen source. The aniline degradation by cells immobilized in alginate, polyvinyl alcohol (PVA) and polyurethane foam (PUF) was significantly better than freely suspended cells. The immobilized AN-5 degraded aniline nearly completely at the initial concentration of 22 mM by 48 hr of incubation. Compared to other binding materials, PUF-immobilized cells were more stable in aniline biodegradation in reused cycles. Moreover, immobilized cells could be stored for three months at 4oC with little reduction of the aniline degradation capacity. These results revealed that PUF-immobilized P. moraviensis AN-5cells has enhanced aniline biodegradation activity. Citation: Ha Danh Duc, Bui Minh Triet, 2017. Biodegradation of aniline by freely suspended and immobilized Pseudomonas moraviensis AN-5. Tap chi Sinh hoc, 39(3): 303-308. DOI: 10.15625/0866-7160/v39n3.9630. *Corresponding author: hadanhduc@gmail.com Received 11 April 2017, accepted 20 August 2017
The status of invasive plants and animals in Cu Lao Cham biosphere reserve, Quang Nam province, Vietnam
Authors: Tai Vu Anh , Khanh Uong Dinh , Anh Luu The , Thu Hien Le Thi 
Published: April 2018

The biodiversity of Cu Lao Cham Biosphere Reserve (Hoi An City, Quang Nam Province) has been faced with some passive impacts, one of which is invasion/expansion of alien species. In 2017, according to the data of GISD, CABI and the Inter-ministerial Circular No.27/2013/TTLT-BTNMT-BNNPTNT, based on filed survey conducted in May, 19 alien plant and 3 alien animal species were recorded in the biosphere reserve. Among them, 13 plant species were identified as invaders, of which details were assessed in this study; among those invader plants, 3 species were ranked at medium risk and the 10 others were ranked at low risk. All of the medium risk-invasive plant species have been appeared on the islands but one of them - siam weed (Chromolaena odorata) were not identified as impacting to the mainland of the biosphere reserve. Likewise, all of the alien animal species have been not recognized as the invasive species. In general, the impact of alien species found in the Cu Lao Cham was assessed as “Low Risk”. The impact status of invasive species in the Hoi An mainland part is more serious than the situation in the islands. Base on the results, we suggest that, five species, beggar-ticks (Bidens pilosa), coast morning glory (Ipomoea cairica) Bay Biscayne creeping-oxeye (Sphagneticola trilobata), Blue porterweed (Stachytarpheta jamaicensis) and billygoat-weed (Ageratum conyzoides) should be added in the invasive appendix of the national invasive species list while three other species as vilfa stellata (Cynodon dactylon), guava (Psidium guava) and rose myrtle (Rhodomyrtus tomentosa) should be listed in the potential appendix of that list. It is necessary to conduct some survey to obtain solution to control invasive species as soon as possible to protect the biodiversity of this study area. Citation: Vu Anh Tai, Uong Dinh Khanh, Luu The Anh, Le Thi Thu Hien, 2017. The status of invasive plants and animals in Cu Lao Cham biosphere reserve, Quang Nam province, Vietnam. Tap chi Sinh hoc, 39(4): 434-450. DOI: 10.15625/0866-7160/v39n4.10082.*Corresponding author: tai.botany@gmail.comReceived 15 June 2017, accepted 12 December 2017
Degradation of leaves and cellulose by Bacillus subtilis CL3 and Pseudomonas aeruginosa CL5 isolated from left waste dumps in Vietnam
Authors: Duc Ha Danh 
Published: April 2018

The biodegradation capacity of two bacterial strains, Bacillus subtilis CL3 and Pseudomonas aeruginosa CL5, isolated from left waste dumps on thee different plant leaves were investigated. The leaves were derived from perennial plants, Malabar almond tree (Terminalia catappa), eucalyptus (Eucalyptus terticornis) and African mahogany (Khaya senegalensis). Among those 3 plants, Eucalyptus terticornis is known to be resistant for biodegradation in nature. In our study, B. subtilis CL3 could degrade a half of Eucalyptus terticornis leaves within 10 days. On the other hand, P. aeruginosa CL5 showed relatively poor degradation rate of the leaves of Eucalyptus terticornis, but could effectively degrade Khaya senegalensis and Terminalia catappa leaves than B. subtilis CL3. When these two bacteria strains were mixed, the degradation rates of all these types of leaves were enhanced. While B. subtilis CL3 could utilize 1,8-cineole, a component extracted from Eucalyptus terticornis, as a sole carbon source, this component inhibited the growth of P. aeruginosa CL5. The multiple and efficient left degradation capacities make these isolates good candidates for bioremediation of leaf waste and cellulose. Citation: Ha Danh Duc, 2017. Degradation of leaves and cellulose by Bacillus subtilis CL3 and Pseudomonas aeruginosa CL5 isolated from left waste dumps in Vietnam. Tap chi Sinh hoc, 39(4): 483-488. DOI: 10.15625/0866-7160/v39n4.10744.*Corresponding author: hadanhduc@gmail.comReceived 5 December 2016, accepted 12 December 2017
Egg-lying behavior of Anisopteromalus calandrae (Howard), an Ectoparasitoid of Lasioderma serricorne (Fabricius)
Authors: Oanh Nguyen Thi , Lan Tran Ngoc , Lam Truong Xuan 
Published: April 2018

This paper describes the egg-laying behavior and reproduction capacity of Anisopteromalus calandrae in laboratory conditions. Anisopteromalus calandrae lay eggs only on the Lasioderma serricorne larvae hiden inside of grains or nests. The maximum lifespan of A. calandrae females was 32 days when they were kept with of aqua feed flour plus honey (30%) and infested with larvae of Lasioderma serricorne. One female laid 71.13 ± 4.24 eggs. The eggs were laid mostly (81.95%) during the first half of the lifespan, with the peak of 7.40 eggs/day on the 9th day. The females usually lay one egg/host larva, but sometimes they lay 2-5 eggs/host larva. They prefer to lay eggs on the 4th larval instar. Citation: Nguyen Thi Oanh, Tran Ngoc Lan, Truong Xuan Lam, 2017. Egg-lying behavior of Anisopteromalus calandrae (Howard), an ectoparasitoid of Lasioderma serricorne (Fabricius). Tap chi Sinh hoc, 39(4): 416-420. DOI: 10.15625/0866-7160/v39n4.10935.* Corresponding author: ntoanh@dthu.edu.vn.Received 17 August 2017, accepted 20 November 2017
Farklı Protein Kaynaklarının Çuka Mersini, Acipenser ruthenus Linnaeus 1758, Juvenillerinde Proteaz Aktivitesi Üzerine Etkileri
Authors: KURT Recep , GÖKÇEK Cemil Kaya 
Published: April 2018

Bu çalışmada, çuka balığı, Acipenser
ruthenus, larvaları yumurtadan çıkış
anından itibaren 29 gün boyunca standart besleme protokolü ile beslenmiştir.
Karma yeme geçiş yapılan 29. gündeki proteolitik aktivite ve farklı protein
kaynaklarının proteaz aktivite üzerine inhibisyon etkileri ölçülmüştür. Çalışmadan
elde edilen bilgiler ışığında, çuka balığı juvenillerinin karma yemlerinde
protein kaynağı olarak soya protein konsantresi (%38,57±10,44) ve mısır gluten (%43,07±1,72) kullanmanın, diğer yem hammaddeleri ile
karşılaştırıldığında, proteolitik enzim aktivitesini istatistiki açıdan daha
olumlu etki yaptığı görülmüştür (P&lt;0,05). Yem hammaddelerinin ikili
kombinasyonları incelendiğinde ise, en düşük inhibisyon oranı balık unu: soya
protein konsantresinin 1:1 oranda kullanılması (%47,93±1,72) ile elde edilmiş
ve bu değerin soya protein konsantresi ve mısır gluteninin tek başlarına
kullanılması ile elde edilen etki ile aynı seviyede olduğu tespit edilmiştir
(P&gt;0,05). En yüksek inhibe edici etki ise, buğday ile pirinç kepeğinin 3:1
oranda kullanılması ile oluşmuştur (%82,39±7,23).
Antrenman Periodizasyonu: Tarihsel Sürecin Bilim Boyutu
Authors: AÇIKADA Caner 
Published: April 2018

The purpose of this brief review is to provide some understanding of developmental processes related with “periodization” and to provide a summary point of view to important contributions in these processes. For this purpose some understanding of the historical events and issues surrounding periodization were provided. Contrary to “Matveyev’s periodization” or “classic periodization” instead of long preparation short competition/match period, which is still valid for some sports, team and acyclic sports and many sports are enjoying more diverse and longer competition and much shorter preparation period. Verkhoshansky accuses Matveyev for accepting “pedagogical adaptation” instead of “biological adaptation” and accepts the “block training” as different training process and tries to compare Matveyev’s “parallel training load” with “block periodization”. Verkhoshansky tries to differentiate between the two training instead of accepting the two supplementing each other. Due to increasing number of competition and longer competition period, the athlete/coach had to strategically choose between the competitions both in individual and team sports. Borg’s 20 scale rating was modified to 10 scale rating trying to create some solutions to some problems encountered in periodization.