bluuebunny/crossref_metadata_2025_split huggingface dataset:
El programa Leader ante la necesidad de impulsar una estrategia de desarrollo más compartida y participativa. Conclusiones derivadas del ejemplo vasco
Authors: Alberdi Collantes Juan Cruz 
Published: December 2024

En líneas generales, los investigadores respaldan el progreso de la iniciativa Leader, incluso aquellos más críticos, reconociendo que pocos programas públicos han logrado resultados tan positivos para el desarrollo rural. Sin embargo, en el caso del País Vasco, que se caracteriza por tener un único grupo que abarca todo su territorio, se observa un programa desprovisto de una estrategia de desarrollo rural clara y supervisado por entidades públicas. A través de conversaciones con diversos actores, se constata que la estrategia promovida está fuertemente limitada por la intervención de la administración, no aborda los desafíos que enfrenta cada territorio ni garantiza la participación de la población local en su diseño e implementación. En contraste, los grupos de acción local en el resto de España se presentan como más receptivos a la participación local y disfrutan de autonomía para definir las líneas de acción. La investigación sugiere elegir un ámbito territorial más coherente y propone el nivel provincial como el más adecuado, abogando por la creación de tres grupos de acción local en el País Vasco. Esto podría fomentar un consenso más sólido en torno a la estrategia a implementar, impulsar la colaboración entre diversos grupos que operan en cada territorio y facilitar la elaboración de una Estrategia de Desarrollo Local más participativa y accesible para la población local.
Effect of Tillage and Weed Management Practices on Growth, Yield Attributes, and Economics of Wheat
Authors: Kumari Shipra , Kumar Birendra , Choudhary S. K. , Singh M.K. , Kumar Sunil , Kumari Kajal , Anand Anurag 
Published: December 2024

A field experiment was conducted during Rabi2023-24 at the research farm, Bihar Agricultural University, Sabour, Bhagalpur, Bihar. The experiment consisted of three tillage operations in main plots and six weed management practices in sub plots laid out in split plot design with three replications. Results revealed that zero tillage with paddy residue led to higher plant height (102.97 cm), leaf area index (2.56), biomass accumulation (570.90 g m-2), no. of spikes m-2(256.45), no. of grains spike-1(49.08), grainyield (3640.86 kg ha-l), net returns (65638 ₹ ha-1) and benefit cost ratio (1.69) which was statistically at par with zero tillage without residue among weed management practices, hand weeding treatment was found significantly superior in all crop traits but it was not economical for farmers due to higher cost of cultivation. However different herbicidal treatments, sulfosulfuron+ metsulfuron methyl (30+2) g a.i. ha-1 were found significantly superior, higher plant height (102.62 cm), leaf area index (2.42), biomass accumulation (580.48 g m-2),no. of spikes m-2(270.36), no. of grains spike-1(49.59), grainyield (3895.68 kg ha-l), net returns(70756 ₹ha-1) and B:C ratio (1.81) which was statistically at par with mesosulfuron methyl + iodosulfuron methyl sodium (12+2.4) g a.i. ha-1.
Near‐Infrared‐Emitting Five‐Monolayer Thick Copper‐Doped CdSe Nanoplatelets
Authors: Sharma Ashma , Sharma Manoj , Gungor Kivanc , Olutas Murat , Dede Didem , Demir Hilmi Volkan 
Published: August 2019

AbstractDoped nanocrystals are instrumental to the high‐performance luminescent solar concentrators (LSCs) and the color conversion devices. Recently, copper (Cu)‐doped three and four monolayer (ML) thick CdSe nanoplatelets (NPLs) have been shown superior to the existing Cu‐doped quantum dots (QDs) for their use in LSCs. However, additional improvement in the LSC performance can be achieved by further redshifting the emission into the near‐infrared (NIR) region of electromagnetic spectrum and increasing the absorbed portion of the solar irradiation. Cu‐doping into higher thicknesses of these atomically flat NPLs (e.g., ≥5 ML) can achieve these overarching goals. However, addition of the dopant ions during the nucleation stage disturbs this high‐temperature growth process and leads to multiple populations of NPLs and QDs. Here, by carefully controlling the precursor chemistry the successful doping of Cu in five ML thick NPLs by high‐temperature nucleation doping method is demonstrated. The optimized synthesis method shows nearly pure population of doped five ML thick NPLs, which possess ≈150 nm Stokes‐shifted NIR emission with high quantum yield of 65 ± 2%. Structural, elemental, and optical studies are conducted to confirm the successful doping and understand the detailed photophysics. Finally, these materials are tested experimentally and theoretically for their performance as promising LSC materials.
One‐Pot Synthesis of Spiropyrans
Authors: Zhang Lei , Deng Yawen , Tang Zhenyu , Zheng Ning , Zhang Chenghao , Xie Congxia , Wu Zhongtao 
Published: August 2019

AbstractA highly efficient one‐pot synthetic strategy for the preparation of spiropyrans was developed. The method is free of special equipment and operations, and meanwhile, it is energy‐ and time‐saving compared to the reported three‐step procedures. Tolerated with various solvents, bases, aldehydes, and alkyl bromides, the commonly used “T‐type” spiropyrans (SP1 s) were synthesized in one‐pot with highly improved yields. To avoid the hydrolysis of the spiropyran moiety during the synthesis of the linear type spiropyrans (SP2 s), a strategy of the combination of pre‐alkylation of phenolic hydroxyl group of 5‐hydroxy‐indole and a one‐pot synthesis of the spiropyran moiety was also designed. With a short reaction time, simplified workups, and reduced chemical waste, this one‐pot synthetic strategy for the synthesis of spiropyran compounds would facilitate related research in the fields of biology and materials science, and would be attractive to the industrial application.
Effect of the month of birth on the height of young adult males
Authors: Douros Konstantinos , Fytanidis Grigorios , Papadimitriou Anastasios 
Published: August 2019

AbstractObjectiveTo explore a possible association between the month of birth and the final adult height in men and see if this could be described in terms of periodicity.Materials and MethodsWe used anthropometric data of 15,109 young male conscripts of the Greek Army. The data were collected from May 2006 until May 2010 and included men who had been born over a period of 12 years (1980–1991). The data were grouped in 12 monthly periods and analyzed with the use of a sinusoidal model.ResultsThere was a statistically significant month‐of‐birth effect on height variation, which was described by a sinusoidal model with period T = 12 months, amplitude 0.223 cm and, two extremes which corresponded to the end of April/beginning of May (peak) and to the end of October/beginning of November (nadir).DiscussionOur results corroborate previous findings suggesting a seasonality in human height without, however, being able to provide a definitive explanation for this phenomenon.
Development of Zinc‐Specific iCEST MRI as an Imaging Biomarker for Prostate Cancer
Authors: Yuan Yue , Wei Zhiliang , Chu Chengyan , Zhang Jia , Song Xiaolei , Walczak Piotr , Bulte Jeff W. M. 
Published: August 2019

AbstractThe healthy prostate contains the highest concentration of mobile zinc in the body. As this level decreases dramatically during the initial development of prostate cancer, in vivo detection of prostate zinc content may be applied for diagnosis of prostate cancer. Using 19F ion chemical exchange saturation transfer magnetic resonance imaging (iCEST MRI) and TF‐BAPTA as a fluorinated Zn‐binding probe with micromolar sensitivity, we show that iCEST MRI is able to differentiate between normal and malignant prostate cells with a 10‐fold difference in contrast following glucose‐stimulated zinc secretion in vitro. The iCEST signal decreased in normal prostate cells upon downregulation of the ZIP1 zinc transporter. In vivo, using an orthotopic prostate cancer mouse model and a transgenic adenocarcinoma of the mouse prostate (TRAMP) model, a gradual decrease of >300 % in iCEST contrast following the transition of normal prostate epithelial cells to cancer cells was detected.
Adaptive soft sensor method based on online selective ensemble of partial least squares for quality prediction of chemical process
Authors: Cang Wentao , Yang Huizhong 
Published: August 2019

AbstractFor accurate state prediction of chemical process characterized by connatural features of non‐linearity and time variation, a novel online selective ensemble partial least squares learning paradigm, referred to as OSE‐PLS, is proposed in this paper. First, the process states are differentiated to diverse local regions by just‐in‐time learning‐based localization method to handle process non‐linearity. Then, support vector domain description algorithm is applied to identify and remove redundant ones. Consequently, a variety of local PLS models are established for representing diverse process states. Inspired by the local outlier probability algorithm in outlier detection field, the combination weights of each PLS model are adaptively distinguished by definitely quantifying the probability that query data belong to an outlier in each local region. Finally, the prediction for query data is achieved through selective ensemble learning strategy. To effectively address the inherent time‐varying characteristics of process, the OSE‐PLS technique is equipped with adaptation mechanism of local model updating and online local model extraction, which enables OSE‐PLS to acquire the capability of addressing both gradual and abrupt changes in process simultaneously. An industrial bisphenol‐A distillation process is employed to demonstrate the superiorities of novel online selective ensemble partial least squares (OSE‐PLS) approach.
Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass
Authors: Čalija Bojan , Milić Jela , Milašinović Nikola , Daković Aleksandra , Trifković Kata , Stojanović Jovica , Krajišnik Danina 
Published: August 2019

ABSTRACTThis study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48406.
Population‐Specific Patterns of Epigenetic Defects in the B Cell Lineage in Patients With Systemic Lupus Erythematosus
Authors: Breitbach Megan E. , Ramaker Ryne C. , Roberts Kevin , Kimberly Robert P. , Absher Devin 
Published: August 2019

ObjectiveTo determine the stage of B cell development at which a systemic lupus erythematosus (SLE)–associated DNA methylation signature originates in African American (AA) and European American (EA) subjects, and to assess whether epigenetic defects in B cell development patterns could be predictive of SLE status in individual and mixed immune cell populations.MethodsB cells from AA patients (n = 31) and EA patients (n = 49) with or without SLE were sorted using fluorescence‐activated cell sorting into 5 B cell subsets. DNA methylation, measured at ~460,000 CpG sites, was interrogated in each subset. Enrichment analysis of transcription factor interaction at SLE‐associated methylation sites was performed. A random forests algorithm was used to identify an epigenetic signature of SLE in the B cell subsets, which was then validated in an independent cohort of AA and EA patients and healthy controls.ResultsRegression analysis across all B cell stages resulted in identification of 60 CpGs that reached genome‐wide significance for SLE‐associated methylation differences (P ≤ 1.07 × 10−7). Interrogation of ethnicity‐specific CpGs associated with SLE revealed a hypomethylated pattern that was enriched for interferon (IFN)–regulated genes and binding of EBF1 in AA patients (each P < 0.001). AA patients with SLE could be distinguished from healthy controls when the predictive model developed with the transitional B cell subset was applied to other B cell subsets (mean receiver operating characteristic [ROC] area under the curve [AUC] 0.98), and when applied to CD19+ pan–B cells (mean ROC AUC 0.95) and CD4+ pan–T cells (mean ROC AUC 0.97) from the independent validation cohort.ConclusionThese results indicate that SLE‐specific methylation patterns are ethnicity dependent. A pattern of epigenetic changes near IFN‐regulated genes early in B cell development is a hallmark of SLE in AA female subjects. EBF1 binding sites are highly enriched for significant methylation changes, implying that this may be a potential regulator of SLE‐associated epigenetic changes.
Using Transitional Changes on High‐Resolution Computed Tomography to Monitor the Impact of Cyclophosphamide or Mycophenolate Mofetil on Systemic Sclerosis–Related Interstitial Lung Disease
Authors: Kim Grace Hyun J. , Tashkin Donald P. , Lo Pechin , Brown Matthew S. , Volkmann Elizabeth R. , Gjertson David W. , Khanna Dinesh , Elashoff Robert M. , Tseng Chi‐Hong , Roth Michael D. , Goldin Jonathan G. 
Published: August 2019

ObjectiveTo examine changes in the extent of specific patterns of interstitial lung disease (ILD) as they transition from one pattern to another in response to immunosuppressive therapy in systemic sclerosis–related ILD (SSc‐ILD).MethodsWe evaluated changes in the quantitative extent of specific lung patterns of ILD using volumetric high‐resolution computed tomography (HRCT) scans obtained at baseline and after 2 years of therapy in patients treated with either cyclophosphamide (CYC) for 1 year or mycophenolate mofetil (MMF) for 2 years in Scleroderma Lung Study II. ILD patterns included lung fibrosis, ground glass, honeycombing, and normal lung. Net change was calculated as the difference in the probability of change from one ILD pattern to another. Wilcoxon's signed rank test was used to compare the changes.ResultsForty‐seven and 50 patients had baseline and follow‐up scans in the CYC and MMF groups, respectively. Mean net improvements reflecting favorable changes from one ILD pattern to another in the whole lung in the CYC and MMF groups, respectively, were as follows: from lung fibrosis to a normal lung pattern, 21% and 19%; from a ground‐glass pattern to a normal lung pattern, 30% and 28%; and from lung fibrosis to a ground‐glass pattern, 5% and 0.5%. The mean overall improvement in transitioning from a ground‐glass pattern or lung fibrosis to a normal lung pattern was significant for both treatments (all P < 0.001).ConclusionSignificantly favorable transitions from both ground‐glass and lung fibrosis ILD patterns to a normal lung pattern were observed in patients undergoing immunosuppressive treatment for SSc‐ILD, suggesting the usefulness of examining these transitions for insights into the underlying pathobiology of treatment response.
A Highly Sensitive and Recyclable Ln‐MOF Luminescent Sensor for the Efficient Detection of Fe3+ and CrVI Anions
Authors: Li Xiao , Tang Jixin , Liu Han , Gao Kuan , Meng Xiangru , Wu Jie , Hou Hongwei 
Published: August 2019

AbstractTwo new three‐dimensional (3D) LnIII metal‐organic frameworks (MOFs) were designed and successfully obtained via a solvothermal reaction between lanthanide(III) nitrates and a semi‐flexible carbazole tetracarboxylate acid linker as a high‐performance chromophore. 1 and 2 possess porous 3D networks with channels along the a axis, and more importantly, they show a highly sensitive and selective fluorescence quenching response to Fe3+ and CrVI anions. The sensing mechanism investigation revealed that the weak interactions of Fe3+ with nitrogen atoms of carbazole and deprotonated carboxylic acids protruding into the pores of MOFs quenched the luminescence of 1 and 2 effectively. In addition, the competition absorption also played an important role in the luminescence quenching detection of Fe3+ based on 1, and CrVI anions based on 1 and 2. Therefore, 1 and 2 represent an alternative example of regenerable luminescence based sensors for the quantitative detection of Fe3+ and CrVI anions.
The XylR variant (R121C and P363S) releases arabinose‐induced catabolite repression on xylose fermentation and enhances coutilization of lignocellulosic sugar mixtures
Authors: Martinez Rodrigo , Flores Andrew D. , Dufault Matthew E. , Wang Xuan 
Published: August 2019

AbstractMicrobial production of fuels and chemicals from lignocellulosic biomass provides a promising alternative to conventional petroleum‐derived routes. However, the heterogeneous sugar composition of lignocellulose prevents efficient microbial sugar co‐fermentation due to carbon catabolite repression, which negatively affects production metrics. We previously discovered that a mutant copy of the transcriptional regulator XylR (P363S and R121C; denoted as XylR*) in Escherichia coli has a higher DNA‐binding affinity than wild‐type XylR, leading to a stronger activation of the d‐xylose catabolic genes and a release from glucose‐induced repression on xylose fermentation. Here, we showed that XylR* also releases l‐arabinose‐induced repression on xylose fermentation through altered transcriptional control, enhancing co‐fermentation of arabinose–xylose sugar mixtures in wild‐type E. coli. Integrating xylR* into an ethanologenic E. coli resulted in the coutilization of 96% of the provided glucose–xylose–arabinose mixtures (120 g/L total sugars supplied) with an ethanol yield higher than 90% of the theoretical maximum by simple batch fermentations.
PD‐L1 expression, CD8+ and CD4+ lymphocyte rate are predictive of pathological complete response after neoadjuvant chemoradiotherapy for squamous cell cancer of the thoracic esophagus
Authors: Fassan Matteo , Cavallin Francesco , Guzzardo Vincenza , Kotsafti Andromachi , Scarpa Melania , Cagol Matteo , Chiarion‐Sileni Vanna , Maria Saadeh Luca , Alfieri Rita , Castagliuolo Ignazio , Rugge Massimo , Castoro Carlo , Scarpa Marco 
Published: August 2019

AbstractBackgroundNeoadjuvant chemoradiotherapy (CTRT) can effectively downstage esophageal squamous cell carcinoma (SCC) in patients with locally advanced disease and prolonged survival have been observed in patients with a pathological complete response (ypCR).Aims and methodsThis exploratory study aimed to identify immunological predictors of pCR after neoadjuvant CTRT within SCC microenvironment. The tumor regression after neoadjuvant therapy was measured according to the Mandard score system. Eighty‐eight consecutive patients with SCC of the thoracic esophagus who received neoadjuvant CTRT were included in this retrospective study. Inclusion criteria were neoadjuvant CTRT and the availability of representative histological samples taken at diagnosis. We investigated immunohistochemical expression of CD4, Tbet, FoxP3, CD8, CD80, PD‐L1, and PD‐1, in the pretreatment biopsies and correlated the immunohistochemical profiles to patients’ outcomes.ResultsAfter neoadjuvant CTRT, 23 patients had pCR, while 65 ones had partial response, stable disease or progression. PD‐L1 expression and CD8+ and CD4+ lymphocyte rate were significantly higher in patients who had ypCR compared to those who had not (10 (0‐55) vs 0 (0‐0), P = 0.004, 73 (36‐147) vs 21 (7‐47), P = 0.0006 and 39 (23‐74) vs 5 (0‐13), P < 0.0001 respectively). The accuracy of expression of PD‐L1+, CD8+, and CD4+ lymphocyte rate in identifying responders was AUC = 0.76 (P = 0.001), AUC = 0.81 (P = 0.0001) and AUC = 0.75 (P = 0.0001), respectively. Within the ypCR group, all patients with high infiltration of CD4+ T cell recurred/relapsed while only the 38.9% of those with low CD4+ T cell infiltration did the same (P = 0.058).ConclusionsPD‐L1 expression and CD8+ and CD4+ lymphocyte rate were predictive of ypCR after neoadjuvant CTRT for SCC of the thoracic esophagus with adequate accuracy. Furthermore, recurrence/relapse was associated with high level of CD4+ T cell infiltration. However, the small sample size prevented to draw definitive conclusions; further studies are necessary to evaluate the prognostic role of these markers.
Sodium nitroprusside induces H‐Ras depalmitoylation and alters the cellular response to hypoxia in differentiated and undifferentiated PC12 cells
Authors: Goloshvili Galina , Barbakadze Tamar , Mikeladze David 
Published: August 2019

Ras‐GTPases regulate many central signalling pathways in the cell. Hypoxia induces nitrosative/oxidative stress and dysregulates Ras‐dependent downstream processes. H‐Ras possesses two cysteine residues (C181 and C184) in the C‐termini, which are palmitoylated once or twice. Palmitoylation is sufficient for promoting stable plasma membrane localization. We hypothesized that high concentrations of hypoxia‐formed nitric oxide could induce terminal cysteine S‐nitrosylation, followed by depalmitoylation and H‐Ras mislocalization. We investigated the action of a 100‐μM nitric oxide‐donor (sodium nitroprusside [SNP]) and a 100‐μM palmitoylation inhibitor (2‐bromopalmitate) on the distribution of membrane‐bound S‐nitrosylated and palmitoylated H‐Ras under hypoxic/normoxic conditions in undifferentiated/differentiated pheochromocytoma (PC12) cells. We found that under normoxic conditions, SNP increases membrane‐bound H‐Ras nitrosylation only in differentiated cells, whereas under hypoxic conditions, SNP stimulates H‐Ras nitrosylation in both differentiated and undifferentiated cells. SNP greatly decreases the palmitoylation of H‐Ras under hypoxic conditions in both undifferentiated and differentiated cells, while under normoxic conditions, the effect of SNP is more negligible. Furthermore, Western blot analyses have shown that SNP decreases ERK phosphorylation under hypoxic conditions, in parallel with an elevation in hypoxia‐induced factor activity and intracellular succinate concentration. We propose that high concentrations of hypoxia‐formed nitric oxide can nitrosylate H‐Ras terminal cysteines, which induce H‐Ras activity dysregulation and alter the cellular response to hypoxia.Significance of the studyTo our knowledge, these observations may be important for cancer prevention and therapy because cancer is one of the most prevalent disorders caused by the misregulation of Ras activity by a redox agent. Oncogenic activation of the H‐Ras gene has been found in a wide variety of neoplastic transformations, and thus, investigation of the redox regulation of H‐Ras activity is significant for cancer research as well.