bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thin Current Sheets of Sub‐ion Scales observed by MAVEN in the Martian Magnetotail
Authors: Grigorenko E. E. , Zelenyi L. M. , DiBraccio G. , Ermakov V. N. , Shuvalov S. D. , Malova H. V. , Popov V. Y. , Halekas J. S. , Mitchell D. L. , Dubinin E. 
Published: June 2019

AbstractCurrent sheets (CSs) play a crucial role in the storage and conversion of magnetic energy in planetary magnetotails. Using high‐resolution magnetic field data from MAVEN spacecraft, we report the existence of super thin current sheets (STCSs) in the Martian magnetotail. The typical half‐thickness of the STCSs is ~5 km, and it is much less than the gyroradius of thermal protons (ρp). The STCSs are embedded into a thicker sheet with L ≥ ρp forming a multiscale current configuration. The formation of STCS does not depend on ion composition, but it is controlled by the small value of the normal component of the magnetic field at the neutral plane (BN). A number of the observed multiscale CSs are located in the parametric map close to the tearing‐unstable domain, and thus, the inner STCS can provide an additional free energy to excite ion tearing mode in the Martian magnetotail.
Larger Increases in More Extreme Local Precipitation Events as Climate Warms
Authors: Li Chao , Zwiers Francis , Zhang Xuebin , Chen Gang , Lu Jian , Li Guilong , Norris Jesse , Tan Yaheng , Sun Ying , Liu Min 
Published: June 2019

AbstractClimate models project that extreme precipitation events will intensify in proportion to their intensity during the 21st century at large spatial scales. The identification of the causes of this phenomenon nevertheless remains tenuous. Using a large ensemble of North American regional climate simulations, we show that the more rapid intensification of more extreme events also appears as a robust feature at finer regional scales. The larger increases in more extreme events than in less extreme events are found to be primarily due to atmospheric circulation changes. Thermodynamically induced changes have relatively uniform effects across extreme events and regions. In contrast, circulation changes weaken moderate events over western interior regions of North America and enhance them elsewhere. The weakening effect decreases and even reverses for more extreme events, whereas there is further intensification over other parts of North America, creating an “intense gets intenser” pattern over most of the continent.
Peatland Volume Mapping Over Resistive Substrates With Airborne Electromagnetic Technology
Authors: Silvestri Sonia , Christensen Craig W. , Lysdahl Asgeir O. K. , Anschütz Helgard , Pfaffhuber Andreas A. , Viezzoli Andrea 
Published: June 2019

AbstractDespite the importance of peatlands as carbon reservoirs, a reliable methodology for the detection of peat volumes at regional scale is still missing. In this study we explore for the first time the use of airborne electromagnetic (AEM) to detect and quantify peat thickness and extension of two bogs located in Norway, where peat lays over resistive bedrock. Our results show that when calibrated using a small amount of field measurements, AEM can successfully detect peat volume even in less ideal conditions, that is, relatively resistive peat over resistive substrata. We expect the performance of AEM to increase significantly in presence of a conductive substratum without need of calibration with field data. The organic carbon content retrieved from field surveys and laboratory analyses combined with the 3‐D model of the peat extracted from AEM allowed us to quantify the total organic carbon of the selected bogs, hence assessing the carbon pool.
OSIRIS‐REx Visible and Near‐Infrared Observations of the Moon
Authors: Simon A. A. , Donaldson Hanna K. L. , Drouet d'Aubigny C. Y. , Poggiali G. , Emery J. P. , Brucato J. , Cosentino R. G. , Reuter D. C. , Golish D. R. , DellaGiustina D. N. , Lunsford A. , Gorius N. , Smith P. H. , Lauretta D. S. 
Published: June 2019

AbstractThe Origins, Spectral Interpretation, Resource Identification, and Security‐Regolith Explorer (OSIRIS‐REx) mission observed the Moon during the spacecraft's Earth gravity assist in 2017. From the spacecraft view, the lunar phase was 42°, and the in‐view hemisphere was dominated by anorthositic highlands terrain. Lunar spectra obtained by the OSIRIS‐REx Visible and InfraRed Spectrometer show evidence of several candidate absorption features. We observe the 2.8‐μm hydration band, confirming the spectral results from other missions, but detected in full‐disk spectra. We also tentatively identify weak spectral features near 0.9 and 1.3 μm, consistent with lunar regolith containing a mixture of plagioclase and orthopyroxene minerals, as expected for highlands terrain.
Isolation and characterization of a thermophilic sulfur- and iron-reducing thaumarchaeote from a terrestrial acidic hot spring
Authors: Kato Shingo , Itoh Takashi , Yuki Masahiro , Nagamori Mai , Ohnishi Masafumi , Uematsu Katsuyuki , Suzuki Katsuhiko , Takashina Tomonori , Ohkuma Moriya 
Published: June 2019

Abstract
A deep-branching clade of Thaumarchaeota, conventionally called Terrestrial hot spring creanarchaeotic group (THSCG), is a missing link between thaumarchaeotic ammonia oxidizers and the deeper-branching non-ammonia oxidizers, such as Crenarchaeota and Candidatus Korarchaeota. Here, we report isolation of the first cultivated representative from the THSCG, named as NAS-02. Physiological characterization demonstrated that the isolate was a thermoacidophilic, sulfur- and iron-reducing organoheterotroph, which was supported by gene contents encoded in its complete genome. There was no evidence for ammonia oxidation by the isolate. Members in THSCG are likely thermophiles, and may play roles in degrading cell debris as a scavenger and in biogeochemical cycling of sulfur and iron in the hot environments, as suggested by the physiological characteristics of the isolate and the geographical distribution of the 16S rRNA gene sequences of THSCG in terrestrial hot springs and marine hydrothermal fields. Phylogenetic analysis suggests that the THSCG lineage represented by NAS-02 has gained the ability of sulfur reduction via horizontal gene transfer. Based on the phylogeny and physiology, we propose the name Conexivisphaera calidus gen. nov., sp. nov. to accommodate the isolate.
Anaerobic hydrocarbon degradation in candidate phylum ‘Atribacteria’ (JS1) inferred from genomics
Authors: Liu Yi-Fan , Qi Zhen-Zhen , Shou Li-Bin , Liu Jin-Feng , Yang Shi-Zhong , Gu Ji-Dong , Mu Bo-Zhong 
Published: June 2019

Abstract
The hydrocarbon-enriched environments, such as oil reservoirs and oil sands tailings ponds, contain a broad diversity of uncultured microorganisms. Despite being one of the few prokaryotic lineages that is consistently detected in both production water from oil reservoirs and stable hydrocarbon-degrading enrichment cultures originated from oil reservoirs, the physiological and ecological roles of candidate phylum “Atribacteria” (OP9/JS1) are not known in deep subsurface environments. Here, we report the expanded metabolic capabilities of Atribacteria as inferred from genomic reconstructions. Seventeen newly assembled medium-to-high-quality metagenomic assembly genomes (MAGs) were obtained either from co-assembly of two metagenomes from an Alaska North Slope oil reservoir or from previous studies of metagenomes coming from different environments. These MAGs comprise three currently known genus-level lineages and four novel genus-level groups of OP9 and JS1, which expands the genomic coverage of the major lineages within the candidate phylum Atribacteria. Genes involved in anaerobic hydrocarbon degradation were found in seven MAGs associated with hydrocarbon-enriched environments, and suggest that some Atribacteria could ferment short-chain n-alkanes into fatty acid while conserving energy. This study expands predicted metabolic capabilities of Atribacteria (JS1) and suggests that they are mediating a key role in subsurface carbon cycling.
Aβ oligomers promote oligodendrocyte differentiation and maturation via integrin β1 and Fyn kinase signaling
Authors: Quintela-López Tania , Ortiz-Sanz Carolina , Serrano-Regal Mari Paz , Gaminde-Blasco Adhara , Valero Jorge , Baleriola Jimena , Sánchez-Gómez Maria Victoria , Matute Carlos , Alberdi Elena 
Published: June 2019

AbstractAlzheimer´s disease (AD) is characterized by a progressive cognitive decline that correlates with the levels of amyloid β-peptide (Aβ) oligomers. Strong evidences connect changes of oligodendrocyte function with the onset of neurodegeneration in AD. However, the mechanisms controlling oligodendrocyte responses to Aβ are still elusive. Here, we tested the role of Aβ in oligodendrocyte differentiation, maturation, and survival in isolated oligodendrocytes and in organotypic cerebellar slices. We found that Aβ peptides specifically induced local translation of 18.5-kDa myelin basic protein (MBP) isoform in distal cell processes concomitant with an increase of process complexity of MBP-expressing oligodendrocytes. Aβ oligomers required integrin β1 receptor, Src-family kinase Fyn and Ca2+/CaMKII as effectors to modulate MBP protein expression. The pharmacological inhibition of Fyn kinase also attenuated oligodendrocyte differentiation and survival induced by Aβ oligomers. Similarly, using ex vivo organotypic cerebellar slices Aβ promoted MBP upregulation through Fyn kinase, and modulated oligodendrocyte population dynamics by inducing cell proliferation and differentiation. Importantly, application of Aβ to cerebellar organotypic slices enhanced remyelination and oligodendrocyte lineage recovery in lysolecithin (LPC)-induced demyelination. These data reveal an important role of Aβ in oligodendrocyte lineage function and maturation, which may be relevant to AD pathogenesis.
Aberrant NFATc1 signaling counteracts TGFβ-mediated growth arrest and apoptosis induction in pancreatic cancer progression
Authors: Hasselluhn Marie C. , Schmidt Geske E. , Ellenrieder Volker , Johnsen Steven A. , Hessmann Elisabeth 
Published: June 2019

AbstractGiven its aggressive tumor biology and its exceptional therapy resistance, pancreatic ductal adenocarcinoma (PDAC) remains a major challenge in cancer medicine and is characterized by a 5-year survival rate of <8%. At the cellular level, PDAC is largely driven by the activation of signaling pathways that eventually converge in altered, tumor-promoting transcription programs. In this study, we sought to determine the interplay between transforming growth factor β (TGFβ) signaling and activation of the inflammatory transcription factor nuclear factor of activated T cells (NFATc1) in the regulation of transcriptional programs throughout PDAC progression. Genome-wide transcriptome analysis and functional studies performed in primary PDAC cells and transgenic mice linked nuclear NFATc1 expression with pro-proliferative and anti-apoptotic gene signatures. Consistently, NFATc1 depletion resulted in downregulation of target genes associated with poor PDAC outcome and delayed pancreatic carcinogenesis in vivo. In contrast to previous reports and consistent with a concept of retained tumor suppressive TGFβ activity, even in established PDAC, TGFβ treatment reduced PDAC cell proliferation and promoted apoptosis even in the presence of oncogenic NFATc1. However, combined TGFβ treatment and NFATc1 depletion resulted in a tremendous abrogation of tumor-promoting gene signatures and functions. Chromatin studies implied that TGFβ-dependent regulators compete with NFATc1 for the transcriptional control of jointly regulated target genes associated with an unfavorable PDAC prognosis. Together, our findings suggest opposing consequences of TGFβ and NFATc1 activity in the regulation of pro-tumorigenic transcription programs in PDAC and emphasize the strong context-dependency of key transcription programs in the progression of this devastating disease.
The alteration of RhoA geranylgeranylation and Ras farnesylation breaks the integrity of the blood–testis barrier and results in hypospermatogenesis
Authors: Zhu Ruilou , Wang Jiangnan , Feng Tianxiang , Hu Xuechun , Jiang Chen , Wang Xiuxing , Li Kang , Sang Yongjuan , Hua Yue , Sun Haixiang , Yao Bing , Li Chaojun 
Published: June 2019

AbstractNon-obstructive azoospermia (NOA) severely affects male infertility, however, the deep mechanisms of this disease are rarely interpreted. In this study, we find that undifferentiated spermatogonial stem cells (SSCs) still exist in the basal compartment of the seminiferous tubules and the blood–testis barrier (BTB) formed by the interaction of neighbor Sertoli cells (SCs) is incomplete in NOA patients with spermatogenic maturation arrest. The adhesions between SCs and germ cells (GCs) are also broken in NOA patients. Meanwhile, the expression level of geranylgeranyl diphosphate synthase (Ggpps), a key enzyme in mevalonate metabolic pathway, is lower in NOA patients than that in obstructive azoospermia (OA) patients. After Ggpps deletion specifically in SCs, the mice are infertile and the phenotype of the SC-Ggpps−/− mice is similar to the NOA patients, where the BTB and the SC–GC adhesions are severely destroyed. Although SSCs are still found in the basal compartment of the seminiferous tubules, fewer mature spermatocyte and spermatid are found in SC-Ggpps−/− mice. Further examination suggests that the defect is mediated by the aberrant protein isoprenylation of RhoA and Ras family after Ggpps deletion. The exciting finding is that when the knockout mice are injected with berberine, the abnormal cell adhesions are ameliorated and spermatogenesis is partially restored. Our data suggest that the reconstruction of disrupted BTB is an effective treatment strategy for NOA patients with spermatogenic maturation arrest and hypospermatogenesis.
Emerging photoluminescence from the dark-exciton phonon replica in monolayer WSe2
Authors: Li Zhipeng , Wang Tianmeng , Jin Chenhao , Lu Zhengguang , Lian Zhen , Meng Yuze , Blei Mark , Gao Shiyuan , Taniguchi Takashi , Watanabe Kenji , Ren Tianhui , Tongay Sefaattin , Yang Li , Smirnov Dmitry , Cao Ting , Shi Su-Fei 
Published: June 2019

AbstractTungsten-based monolayer transition metal dichalcogenides host a long-lived “dark” exciton, an electron-hole pair in a spin-triplet configuration. The long lifetime and unique spin properties of the dark exciton provide exciting opportunities to explore light-matter interactions beyond electric dipole transitions. Here we demonstrate that the coupling of the dark exciton and an optically silent chiral phonon enables the intrinsic photoluminescence of the dark-exciton replica in monolayer WSe2. Gate and magnetic-field dependent PL measurements unveil a circularly-polarized replica peak located below the dark exciton by 21.6 meV, equal to E″ phonon energy from Se vibrations. First-principles calculations show that the exciton-phonon interaction selectively couples the spin-forbidden dark exciton to the intravalley spin-allowed bright exciton, permitting the simultaneous emission of a chiral phonon and a circularly-polarized photon. Our discovery and understanding of the phonon replica reveals a chirality dictated emission channel of the phonons and photons, unveiling a new route of manipulating valley-spin.
The observation of vibrating pear-shapes in radon nuclei
Authors: Butler P. A. , Gaffney L. P. , Spagnoletti P. , Konki J. , Scheck M. , Smith J. F. , Abrahams K. , Bowry M. , Cederkäll J. , Chupp T. , de Angelis G. , De Witte H. , Garrett P. E. , Goldkuhle A. , Henrich C. , Illana A. , Johnston K. , Joss D. T. , Keatings J. M. , Kelly N. A. , Komorowska M. , Kröll T. , Lozano M. , Nara Singh B. S. , O’Donnell D. , Ojala J. , Page R. D. , Pedersen L. G. , Raison C. , Reiter P. , Rodriguez J. A. , Rosiak D. , Rothe S. , Shneidman T. M. , Siebeck B. , Seidlitz M. , Sinclair J. , Stryjczyk M. , Van Duppen P. , Vinals S. , Virtanen V. , Warr N. , Wrzosek-Lipska K. , Zielinska M. 
Published: June 2019

AbstractThere is a large body of evidence that atomic nuclei can undergo octupole distortion and assume the shape of a pear. This phenomenon is important for measurements of electric-dipole moments of atoms, which would indicate CP violation and hence probe physics beyond the Standard Model of particle physics. Isotopes of both radon and radium have been identified as candidates for such measurements. Here, we observed the low-lying quantum states in 224Rn and 226Rn by accelerating beams of these radioactive nuclei. We show that radon isotopes undergo octupole vibrations but do not possess static pear-shapes in their ground states. We conclude that radon atoms provide less favourable conditions for the enhancement of a measurable atomic electric-dipole moment.
Publisher Correction: RdDM-independent de novo and heterochromatin DNA methylation by plant CMT and DNMT3 orthologs
Authors: Yaari Rafael , Katz Aviva , Domb Katherine , Harris Keith D. , Zemach Assaf , Ohad Nir 
Published: June 2019

The original version of this Article contained an error in Fig. 5, in which the evolutionary origin of DRM2 was incorrectly placed prior to the divergence between gymnosperms and angiosperms . The correct evolutionary origin of DRM2 should be in angiosperms. In addition, in the “Percent methylation change” section of the Methods, Equation 1 was incorrect. This has been corrected in both the PDF and HTML versions of the Article.An amendment to this paper has been published and can be accessed via a link at the top of the paper
Glutamate induces synthesis of thrombogenic peptides and extracellular vesicle release from human platelets
Authors: Gautam Deepa , Tiwari Arundhati , Nath Chaurasia Rameshwar , Dash Debabrata 
Published: June 2019

AbstractPlatelets are highly sensitive blood cells, which play central role in hemostasis and thrombosis. Platelet dense granules carry considerable amount of neurotransmitter glutamate that is exocytosed upon cell activation. As platelets also express glutamate receptors on their surface, it is pertinent to ask whether exposure to glutamate would affect their signalling within a growing thrombus. In this study we demonstrate that, glutamate per se induced synthesis of thrombogenic peptides, plasminogen activator inhibitor-1 and hypoxia-inducible factor-2α, from pre-existing mRNAs in enucleate platelets, stimulated cytosolic calcium entry, upregulated RhoA-ROCK-myosin light chain/myosin light chain phosphatase axis, and elicited extensive shedding of extracellular vesicles from platelets. Glutamate, too, incited platelet spreading and adhesion on to immobilized matrix under arterial shear, raised mitochondrial transmembrane potential associated with generation of reactive oxygen species and induced activation of AMP-activated protein kinase in platelets. Taken together, glutamate switches human platelets to pro-activation phenotype mediated mostly through AMPA receptors and thus targeting glutamate receptors may be a promising anti-platelet strategy.
LncRNA PTCSC3 inhibits cell proliferation in laryngeal squamous cell carcinoma by down-regulating lncRNA HOTAIR
Authors: Xiao Dong , Cui Xiangyan , Wang Xin 
Published: June 2019

Abstract
It is known that lncRNA PTCSC3 inhibits thyroid cancer and glioma and STAT3 promotes cancer development. We, in the present study, investigated the potential involvement of PTCSC3 in laryngeal squamous cell carcinoma (LSCC) and explored its interactions with STAT3. In the present study, we showed that plasma PTCSC3 was down-regulated in early stage LSCC patients, and the down-regulation of PTCSC3 separated in early stage LSCC patients from control group. LncRNA HOTAIR was up-regulated in early stage LSCC patients and was significantly and inversely correlated with PTCSC3 in LSCC patients. PTCSC3 overexpression led to the inhibition of HOTAIR, while PTCSC3 expression was not significantly affected by HOTAIR overexpression. PTCSC3 overexpression mediated the inhibited, while HOTAIR overexpression mediated the promoted proliferation of LSCC cells. However, cell invasion and migration were not significantly affected by PTCSC3 overexpression. In addition, HOTAIR overexpression reduced the inhibitory effects of PTCSC3 overexpression on cancer cell proliferation. Moreover, PTCSC3 overexpression mediated the down-regulation of STAT3 and STAT3 overexpression mediated the up-regulation of HOTAIR. Therefore, PTCSC3 may negatively interact with HOTAIR through STAT3 to inhibit LSCC cell proliferation.
MiR-876-5p regulates gastric cancer cell proliferation, apoptosis and migration through targeting WNT5A and MITF
Authors: Xu Zhenglei , Yu Zhichao , Tan Qinghong , Wei Cheng , Tang Qi , Wang Lisheng , Hong Yingcai 
Published: June 2019

Abstract
MicroRNAs (miRNAs) are reported to play critical roles in various cancers. Recently, mounting miRNAs are found to exert oncogenic or tumor inhibitory role in gastric cancer (GC), however, their potential molecular mechanism in GC remains ill-defined. Currently, we aimed to elucidate the functional and mechanistic impacts of a novel miRNA on GC cellular process. The significant down-regulation of miR-876-5p in GC cells attracted our attention. In function, we performed gain-of-function assays and found that miR-876-5p overexpression repressed proliferative, anti-apoptotic and migratory abilities and epithelial–mesenchymal transition (EMT) of GC cells. By applying bioinformatics prediction and mechanism experiments, we verified that miR-876-5p could double-bind to the 3′ untranslated regions (3′UTRs) of Wnt family member 5A (WNT5A) and melanogenesis associated transcription factor (MITF), thus regulating their mRNA and protein levels. Both WNT5A and MITF were highly expressed in GC cells. Additionally, we conducted loss-of-function assays and confirmed the oncogenic roles of WNT5A and MITF in GC. Finally, rescue assay uncovered a fact that miR-876-5p suppressed GC cell viability and migration, but induced cell apoptosis via targeting WNT5A and MITF. Taken together, we might offer a valuable evidence for miR-876-5p role in GC development.
Association of functional variant in GDF1 promoter with risk of congenital heart disease and its regulation by Nkx2.5
Authors: Gao Xiaobo , Zheng Panpan , Yang Liping , Luo Haiyan , Zhang Chen , Qiu Yongqiang , Huang Guoying , Sheng Wei , Ma Xu , Lu Cailing 
Published: June 2019

Abstract
GDF1 plays an important role in left–right patterning and genetic mutations in the coding region of GDF1 are associated with congenital heart disease (CHD). However, the genetic variation in the promoter of GDF1 with sporadic CHD and its expression regulation is little known. The association of the genetic variation in GDF1 promoter with CHD was examined in two case–control studies, including 1084 cases and 1198 controls in the first study and 582 cases and 615 controls in the second study. We identified one single nucleotide polymorphism (SNP) rs181317402 and two novel genetic mutations located in the promoter region of GDF1. Analysis of combined samples revealed a significant association in genotype and allele frequencies of rs181317402 T/G polymorphism between CHD cases in overall or ventricular septal defects or Tetralogy of Fallot and the control group. rs181317402 allele G polymorphism was significantly associated with a decreased risk of CHD. Furthermore, luciferase assay, chromatin immunoprecipitation and DNA pulldown assay indicated that Nkx2.5 transactivated the expression of GDF1 by binding to the promoter of GDF1. Luciferase activity assay showed that rs181317402 allele G significantly increased the basal and Nkx2.5-mediated activity of GDF1 promoter, while the two genetic mutations had the opposite effect. rs181317402 TG genotype was associated with significantly increased mRNA level of GDF1 compared with TT genotype in 18 CHD individuals. Our results demonstrate for the first time that Nkx2.5 acts upstream of GDF1 and the genetic variants in GDF1 promoter may confer genetic susceptibility to sporadic CHD potentially by altering its expression.