bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-occurring alterations in the RAS-MAPK pathway limit response to MET inhibitor treatment inMETexon 14 skipping mutation positive lung cancer
Authors: Rotow Julia K. , Gui Philippe , Wu Wei , Raymond Victoria M. , Lanman Richard B. , Kaye Frederic J. , Peled Nir , de la Cruz Ferran Fece , Nadres Brandon , Corcoran Ryan B. , Yeh Iwei , Bastian Boris C. , Starostik Petr , Newsom Kimberly , Olivas Victor R , Wolff Alexander M. , Fraser James S. , Collisson Eric A. , McCoach Caroline E. , Camidge D. Ross , Pacheco Jose , Bazhenova Lyudmila , Li Tianhong , Bivona Trever G. , Blakely Collin M. 
Published: July 2018

AbstractPURPOSEWhile patients with advanced-stage non-small cell lung cancers (NSCLCs) harboringMETexon 14 skipping mutations (METex14) often benefit from MET tyrosine kinase inhibitor (TKI) treatment, clinical benefit is limited by primary and acquired drug resistance. The molecular basis for this resistance remains incompletely understood.METHODSTargeted sequencing analysis was performed on cell-free circulating tumor DNA obtained from 289 patients with advanced-stageMETex14-mutated NSCLC.RESULTSProminent co-occurring RAS-MAPK pathway gene alterations (e.g. inKRAS, NF1) were detected in NSCLCs withMETex14 skipping alterations as compared toEGFR-mutated NSCLCs. There was an association between decreased MET TKI treatment response and RAS-MAPK pathway co-occurring alterations. In a preclinical model expressing a canonicalMETex14 mutation, KRAS overexpression or NF1 downregulation hyperactivated MAPK signaling to promote MET TKI resistance. This resistance was overcome by co-treatment with crizotinib and the MEK inhibitor trametinib.CONCLUSIONOur study provides a genomic landscape of co-occurring alterations in advanced-stageMETex14-mutated NSCLC and suggests a potential combination therapy strategy targeting MAPK pathway signaling to enhance clinical outcomes.
Assessment of Auditable Pharmaceutical Transactions at Public Hospitals in Gamo Gofa, Southern Ethiopia, a Comparative cross-sectional study, July 2017
Authors: Mensa Mende , Ayele Akililu , Wogayehu Biruk 
Published: July 2018

AbstractBackgroundAvailability of essential medicines is necessary to maintain health of the community. In Ethiopia, availability of medicines was low (65%), with high expiry rate (8.24%), low patient knowledge on correct dosage (50.5%) and satisfaction on pharmacy services (74.5%). To avert these problems, the government had endorsed legislation on a system called “Auditable Pharmaceutical Transactions and Services (APTS)”. However, the outcomes and challenges in implementation of this system were not assessed.ObjectiveTo assess the implementation status of APTS and its challenges at public hospitals in Gamo Gofa Zone Southern Ethiopia, April 2017.MethodsFacility based Cross sectional study was conducted in two APTS implementing hospitals in Gamo Gofa zone. Semi structured Self-administered questionnaire was distributed to all pharmacy staffs in selected hospitals. APTS reports of 12 months (with different characteristics) were reviewed. Four hundred patients were interviewed by data collectors about patient knowledge and satisfaction using WHO questionnaire. The data were entered and analyzed using Statistical package for social science students/SPSS version 20. T-test and linear regression was used to evaluate significant differences between two hospitals with level of significance pre-set at p-value ≤0.05.ResultsAll dispensing units in primary hospital had six (75%) out of the eight essential equipment for dispensing practice. it was found that respondents in general hospital stated higher scores in general setting of outpatient pharmacy (4.58 Versus 4.25; P <0.001), but lower scores for availability and cost of medicines (4.24 vs 4.43; P<0.05) when compared with those in primary level hospital. There was no significant difference in instruction of medicine provided by dispenser (2.58 vs 2.59; P>0.05), dispenser client interaction (3.09 vs 4.08; P>0.05) and total satisfaction score (2.09 vs 2.02; P>0.05).Conclusion and recommendationsIn our study Quality of Auditable Pharmaceutical Transactions and Service was low, especially regarding patient knowledge about medicines, unaffordability of medicines, less availability of prescribed drugs, poor transparency of pharmaceutical transactions, insufficient counseling practice and limited facilities for dispensing such as, key medicines, formularies and standard guidelines. We therefore recommend the following measures responsible bodies to improve these gaps y taking administrative actions and providing continued education and training for dispensers.
Microbiota composition and evenness predict survival rate of oysters confronted to Pacific Oyster Mortality Syndrome
Authors: Clerissi Camille , de Lorgeril Julien , Petton Bruno , Lucasson Aude , Escoubas Jean-Michel , Gueguen Yannick , Dégremont Lionel , Mitta Guillaume , Toulza Eve 
Published: July 2018

AbstractPacific Oyster Mortality Syndrome (POMS) affects Crassostrea gigas oysters worldwide and caused important economic losses. Disease dynamics was recently deciphered and revealed a multiple and progressive infection caused by the Ostreid herpesvirus OsHV-1 µVar, triggering an immunosuppression followed by microbiota destabilization and bacteraemia by opportunistic bacterial pathogens. However, it remains unknown if microbiota might participate to oyster protection to POMS, and if microbiota characteristics might be predictive of oyster mortalities. To tackle this issue, we transferred full-sib progenies of resistant and susceptible oyster families from hatchery to the field during a period in favour of POMS. After five days of transplantation, oysters from each family were either sampled for individual microbiota analyses using 16S rRNA gene-metabarcoding or transferred into facilities to record their survival using controlled condition. As expected, all oysters from susceptible families died, and all oysters from the resistant family survived. Quantification of OsHV-1 and bacteria showed that five days of transplantation was long enough to contaminate oysters by POMS, but not for entering the pathogenesis process. Thus, it was possible to compare microbiota characteristics between resistant and susceptible oyster families at the early steps of infection. Strikingly, we found that microbiota evenness and abundances of Cyanobacteria (Subsection III, family I), Mycoplasmataceae, Rhodobacteraceae, and Rhodospirillaceae were significantly different between resistant and susceptible oyster families. We concluded that these microbiota characteristics might predict oyster mortalities.
Sex differences in hippocampal cytokine networks after systemic immune challenge
Authors: Finnell Julie E. , Speirs Ian C. , Tronson Natalie C. 
Published: July 2018

ABSTRACTIncreased production of cytokines in the in the brain during illness or injury modulates physiological processes, behavior, and cognitive function. It is likely that the pattern of cytokines, rather than the activation of any individual cytokine, determines the functional outcome of neuroimmune signaling. Cytokine networks may thus be particularly useful for understanding sex differences in immune and neuroimmune activation and outcomes. In this project, we aimed to determine the activation and resolution of hippocampal cytokine networks in both male and female mice. We measured 32 cytokines in the hippocampus and periphery of male and female mice at rest, 2, 6, 24, 48, and 168 hours after an acute systemic injection of lipopolysaccharide (LPS; 250μg/kg). We hypothesized that males and females would exhibit both differences in individual cytokine levels and differences in network dynamics of hippocampal cytokines. Cytokines with sex-specific activation by LPS included male-specific elevations of IFNɣ, CSF1, CSF2, and IL-10; and female-specific activation of the IL-2 family and IL-4. We also observed differences in time course, where females showed more rapid elevations, and faster resolution of cytokine activity compared with males. Network analysis using ARACNE and Cytoscape demonstrated markedly different hippocampal cytokine networks across sex even at baseline, and sex differences in cytokine network activation states in response to LPS. Analysis of global shifts in cytokine concentrations further identified a period of cytokine and chemokine downregulation at 48 hours that was more pronounced in females compared with males. Together, these findings demonstrate that sex differences in neuroimmune responses include both differences in intensity of the cytokine response, and importantly differences in cytokine networks activated. Such sex differences in cytokine networks in the brain are likely critical for short and long-term functional outcomes associated with neuroimmune activation.
CRISPR-induced deletion with SaCas9 restores dystrophin expression in dystrophic models in vitro and in vivo
Authors: Duchêne Benjamin L. , Cherif Khadija , Iyombe-Engembe Jean-Paul , Guyon Antoine , Rousseau Joel , Ouellet Dominique L. , Barbeau Xavier , Lague Patrick , Tremblay Jacques P. 
Published: July 2018

AbstractDuchenne Muscular Dystrophy (DMD), a severe hereditary disease, affecting 1 boy out of 3500, mainly results from the deletion of one or more exons leading to a reading frame shift of the DMD gene that abrogates dystrophin protein synthesis. We used the Cas9 of Staphylococcus aureus (SaCas9) to edit the human DMD gene. Pairs of sgRNAs were meticulously chosen to induce a genomic deletion to not only restore the reading frame but also produced a dystrophin protein with normally phased spectrin-like repeats. The formation of a dystrophin protein with spectrin-like repeats normally phased is not usually obtained by skipping or by deletion of complete exons. This can however be obtained in rare instances where the exon/intron borders of the beginning and the end of the complete deletion (patient deletion plus CRISPR-induced deletion are at similar positions in the spectrin-like repeat. We used pairs of sgRNAs, targeting exons 47 and 58 and a normal reading frame was restored in 67 to 86% of the resulting hybrid exons in myoblasts derived from muscle biopsies of 4 DMD patients with different exon deletions. The restoration of the DMD reading frame and restoration of the dystrophin expression was also obtained in vivo in the heart of the del52hDMD/mđx. Our results provide a proof-of-principle that SaCas9 could be used to edit the human DMD gene and could be considered for the further development of a therapy for DMD.
The content of selected nutrients and minerals in some cultivars of Cucurbita maxima
Authors: Czech Anna , Stępniowska Anna , Wiącek Dariusz , Sujak Agnieszka , Grela Eugeniusz Ryszard 
Published: July 2018

Purpose
The purpose of this paper is to compare the content of nutrients and minerals in various cultivars of Cucurbita maxima.

Design/methodology/approach
This is preliminary study, so eight samples from each cultivar of pumpkin used in this study were obtained from local farms from Poland. The following pumpkin cultivars were used: Australian butter, Flat white boer, Garbo, Golden delicious, Golden nugget, Hokkaido, Solor and Zapallito de tronco. In pumpkins elements, dry matter, ash and crude protein were determined. All elements were determined using Inductively Coupled Plasma Optical Emission Spectrometer. Content of dry matter, ash and crude protein according to AOAC methods.

Findings
The fruit of Cucurbita maxima, irrespective of cultivar, is a good means of supplementing the diet with highly beneficial fibre, and minerals such as potassium, calcium, phosphorus, magnesium, sulphur, silicon, iron and zinc. The pumpkins richest in minerals are those of the Australian butter cultivar (calcium, manganese, iron), Flat white boer (sodium, silicon, chromium, nickel), Hokkaido (potassium, phosphorus, zinc, chromium) and Solor (magnesium, sulphur, copper), while the Garbo, Golden Delicious and Zapallito de tronco cultivars had the lowest concentrations of minerals.

Originality/value
The results obtained indicate that depending on the consumer’s expectations, pumpkins of various cultivars can be a dietary component that corrects deficiencies in the diet. Pumpkins grown in Poland have been characterised in terms of the content of mineral elements. These vegetables have become an important component of local food in Poland.
Factors associated with the consumption of traditional foods in central Mexico
Authors: Serrano-Cruz Marlem R. , Espinoza-Ortega Angélica , Sepúlveda Wilmer S. , Vizcarra-Bordi Ivonne , Thomé-Ortiz Humberto 
Published: July 2018

Purpose
The purpose of this paper is to identify the factors associated with the consumption of traditional foods (TFs) in central Mexico.

Design/methodology/approach
A total of 512 surveys were applied to consumers in central Mexico. The survey included a free word association with TF and an example, the food choice questionnaire, and the sociodemographic characteristics of respondents. A binary logistic regression was carried out comparing regular TF consumers with non-regular TF consumers.

Findings
Men tend to eat TFs in more regular way than women. People with heavier consumption habits associate TF with “authenticity/way of doing”, “origin” and “habit”, which are reinforced both by TF examples and the FCQ variables. Foods considered traditional were classified as cooked (elaborated under specific recipes of gastronomic heritage) and uncooked. Both groups comprise central foods, basic in the Mexican diet, in which maize, chili and beans are outstanding.

Research limitations/implications
The study did not comprehend the whole country and not consider specific food categories.

Practical implications
Provides important information on the factors that drive consumption of TFs in Mexico, information that may be useful in valorisation processes.

Social implications
Results may support the commercialisation of these foods, and lead to an improvement in the livelihoods of producers of TFs.

Originality/value
In Mexico, the study of consumers has not been addressed. Therefore, this is a pioneer study that documents the factors associated with the consumption of TFs.
Determinants of pesticide application: an empirical analysis with theory of planned behaviour
Authors: Wang Jianhua , Chu May , Deng Yuan yuan , Lam Honming , Tang Jianjun 
Published: July 2018

Purpose
The purpose of this paper is to investigate farmers’ intentions to comply with pesticide application standards based on an extended theory of planned behaviour (TPB).

Design/methodology/approach
Built on a TPB framework, it was examined how perceived behavioural control (PBC), behavioural goal (BG), behavioural attitude (BA) and subjective norm (SN) influenced farmers’ intention to comply with pesticide application standards. Data of 986 farmers from five major agricultural provinces in China were collected following a stratified random sampling method. Structural equation modelling was employed for hypothesis testing and analysis.

Findings
The results showed that PBC, BG, behavioural attitude and SN had positive impacts on farmers’ intention in abiding by the standards. Among them in determining farmers’ intention towards compliance with pesticide application standards, farmers’ PBC was found to be the most influential factor, while SN was the least influential factor.

Originality/value
The results indicated that the traditional TPB constructs had significant correlations with farmers’ intention to comply with pesticide standards, demonstrating the applicability of the TPB in the understanding of farmers’ decision-making in a developing country context. It is suggested that psychological factors should be taken into consideration in studying farmers’ decision-making.
Changes in returns to education for off-farm wage employment: evidence from rural China
Authors: Wang Weidong , Dong Yongqing , Luo Renfu , Bai Yunli , Zhang Linxiu 
Published: July 2018

PurposeThe purpose of this paper is to examine the role of education in the labor market and to understand how returns to education change over time in rural China.Design/methodology/approachUsing nationally representative survey data from 2004 to 2015, this study provides insights on wage determination in the labor market and examines how the returns to education in rural China differ with time and educational endowment. This study applies ordinary least squares estimation and the Heckman selection model to estimate the returns to education.FindingsThe returns to education decreased during the observed years from more than 6 percent in 2004 to only about 3 percent in 2011, rising to nearly 4 percent in 2015. The overall trend is robust and observed within groups defined by education. Additionally, the returns to education vary greatly with educational endowment. Tertiary education has always maintained a high rate of returns at nearly 10 percent, while returns to senior high school education and below have gradually diminished.Originality/valueThe authors believe that the results will not only enrich studies on the returns to education in rural China, but also provide a basis for diagnosing the changes of rural labor market in the early twenty-first century.
Relationship between perceived justice and identification
Authors: Bergami Massimo , Morandin Gabriele 
Published: July 2018

PurposeThe purpose of this paper is to contribute to our understanding of the antecedents of organizational identification. Specifically, this paper aims to integrate two perspectives developed within the social identity domain, labeled “cognitive” and “relational,” by comparing and reconciling their relationship organizational identification.Design/methodology/approachThis study uses a survey method and a structured questionnaire to collect data from people working in a call center. The hypotheses were tested on a sample of 743 employees by using structural equation models and Hayes’ (2017) bootstrapping procedure.FindingsThe results provide evidence for a mediational model in which the attractiveness of organizational images (cognitive representations) mediates the relationship between perceived justice (relational judgments) and organizational identification.Research limitations/implicationsThe data were obtained from a single source in a cross-sectional design, which may inflate common method variance. To address threats to validity, the authors employed several procedures, the results of which revealed that no parameters corresponding to the hypotheses changed in sign or significance, thus suggesting that the presence of method bias, if any, was nonconsequential.Practical implicationsNot only does perceived justice relate to the sense of belonging to an organization, but it also contributes to shaping the long-term cognitive representations of the company. In particular, both HR and line managers should be aware that in this respect, the interactional dimension of justice shows the strongest effect.Originality/valueBuilding on and enlarging the scope of the extant literature, the findings contribute to our knowledge of how relational judgments shape cognitive images about the company, influencing, in turn, the individual–organization relationship.
Tribological behavior of AA7075-TiC composites by powder metallurgy
Authors: C. Saravanan , K. Subramanian , V. Anandakrishnan , S. Sathish 
Published: July 2018

Purpose
Aluminium is the most preferred material in engineering structural components because of its excellent properties. Furthermore, the properties of aluminium may be enhanced through metal matrix composites and an in-depth investigation on the evolved properties is needed in view of metallurgical, mechanical and tribological aspects. The purpose of this study is to explore the effect of TiC addition on the tribological behavior of aluminium composites.

Design/methodology/approach
Aluminium metal matrix composites at different weight percentage of titanium carbide were produced through powder metallurgy. Produced composites were subjected to sliding wear test under dry condition through Taguchi’s L9 orthogonal design.

Findings
Optimal process condition to achieve the minimum wear rate was identified though the main effect plot. Sliding velocity was identified as the most dominating factor in the wear resistance.

Practical implications
The production of components with improved properties is promoted efficiently and economically by synthesizing the composite via powder metallurgy.

Originality/value
Though the investigations on the wear behavior of aluminium composites are analyzed, reinforcement types and the mode of fabrication have their significance in the metallurgical and mechanical properties. Thus, the produced component needs an in-detail study on the property evolution.
Place attachment and challenges of historic cities
Authors: Garcia Gabriela , Vandesande Aziliz , van Balen Koenraad 
Published: July 2018

Purpose
The effect of heritage on place attachment is assessed by understanding how people “value” their heritage environment. In addition, the purpose of this paper is to assess the effects of place attachment on residential and investment trends according to two different social groups: local inhabitants and foreign immigrants.

Design/methodology/approach
A single case study design for Santa Ana de los Ríos de Cuenca including an open-ended survey, combined with Likert-scale analysis. The survey protocol and questions for the respondent groups were structured according to place identity, place dependency and sense of place.

Findings
The analysis bridges the gap between theory and practice and demonstrates interesting similarities as well as differences between both foreign and local respondent groups in terms of the attributes they consider as cultural heritage and their preferences in terms of residential or investment location choice. The research demonstrates that an increased understanding of place attachment as the link between place dependency and sense of place in a specific location can assist in defining effective built heritage policies in favour of local sustainability.

Originality/value
The analysis of place attachment in relation to the origin of respondents revealed different perceptions on the role that a heritage environment might play on attracting or influencing displacement of local inhabitants and foreign immigrants. Based on original empirical data collection in Cuenca, Ecuador, this study identified research lines and actions which should be prioritised for improving management of this World Heritage Site.
An analysis of auction strategies in apartment sales
Authors: Hungria-Gunnelin Rosane 
Published: July 2018

PurposeThis paper aims to empirically test the effect of list price and bidding strategies in ascending auctions of residential real estate.Design/methodology/approachThree regression models are estimated, using a unique data set from 629 condominium apartments in the inner-city of Stockholm, Sweden, sold between January 2010 and December 2011.FindingsThe results show that jump bidding has the predicted effect of reducing competition by scaring off bidders. However, a higher average bid increment leads to a higher selling price. Furthermore, results show that a fast auction in terms of average time between bids acts to increase the probability of so-called auction fever as both the number of bidders and the selling price are positively correlated with the speed of the auction. While the average behavior of all auction participants, in terms of jump bidding and time between bids, significantly affects auction outcomes, differences in strategies applied by winners and losers show mixed results. The results of this study with respect to sellers’ list price strategy show that underpricing is an ineffective strategy in terms of enticing more bidders to participate in the auction. Furthermore, underpricing is not sufficient to have a positive effect on the selling price.Originality/valueThis paper is one of the first papers to empirically analyze how different bidding strategies affect the outcome of residential real estate auctions in terms of competition and the final selling price.