bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Recurrent Activating Missense Mutation in Waldenström Macroglobulinemia Affects the DNA Binding of the ETS Transcription Factor SPI1 and Enhances Proliferation
Authors: Roos-Weil Damien , Decaudin Camille , Armand Marine , Della-Valle Véronique , Diop M'boyba K. , Ghamlouch Hussein , Ropars Virginie , Hérate Cécile , Lara Diane , Durot Eric , Haddad Rima , Mylonas Elena , Damm Frederik , Pflumio Francoise , Stoilova Bilyana , Metzner Marlen , Elemento Olivier , Dessen Philippe , Camara-Clayette Valérie , Cosset François-Loïc , Verhoeyen Els , Leblond Véronique , Ribrag Vincent , Cornillet-Lefebvre Pascale , Rameau Philippe , Azar Nabih , Charlotte Frédéric , Morel Pierre , Charbonnier Jean-Baptiste , Vyas Paresh , Mercher Thomas , Aoufouchi Said , Droin Nathalie , Guillouf Christel , Nguyen-Khac Florence , Bernard Olivier A. 
Published: April 2019

Abstract

The ETS-domain transcription factors divide into subfamilies based on protein similarities, DNA-binding sequences, and interaction with cofactors. They are regulated by extracellular clues and contribute to cellular processes, including proliferation and transformation. ETS genes are targeted through genomic rearrangements in oncogenesis. The PU.1/SPI1 gene is inactivated by point mutations in human myeloid malignancies. We identified a recurrent somatic mutation (Q226E) in PU.1/SPI1 in Waldenström macroglobulinemia, a B-cell lymphoproliferative disorder. It affects the DNA-binding affinity of the protein and allows the mutant protein to more frequently bind and activate promoter regions with respect to wild-type protein. Mutant SPI1 binding at promoters activates gene sets typically promoted by other ETS factors, resulting in enhanced proliferation and decreased terminal B-cell differentiation in model cell lines and primary samples. In summary, we describe oncogenic subversion of transcription factor function through subtle alteration of DNA binding leading to cellular proliferation and differentiation arrest.

Significance:
The demonstration that a somatic point mutation tips the balance of genome-binding pattern provides a mechanistic paradigm for how missense mutations in transcription factor genes may be oncogenic in human tumors.
This article is highlighted in the In This Issue feature, p. 681
Levels of Geometrical Thinking of Students Receiving Blended Learning in Jordan
Authors: Altakhyneh Bahjat Hamid 
Published: April 2019

The study aimed at investigating the levels of geometrical thinking among students receiving blended learning in Jordan. The study sample consisted of (104) students / teachers of open education systems in Jordan for the2015 -2016 academic year. In order to answer the questions of study, the researcher developed a scale of geometric thinking, it is validity and reliability has been verified. The results of the study showed a low level of geometrical thinking among students receiving blended learning. The percentage of students in the first level (visual) (51%), the percentage of students in the second level (descriptive) (15%), and the percentage of students in the third level (logical) (3%). Also it showed differences in the levels of geometric thinking between males and females in favor of males, as well as differences in the level of geometrical thinking between students from the scientific stream and students from the literary stream in favor of scientific stream. In the light of the results of the study, the researcher recommends that pre-service teachers should be trained on programs contains geometrical thinking at open learning universities.
Needs analysis on Indonesian language learning materials about agriculture for foreign speakers
Authors: Usmansyah Defina , Rasyid Yumna , Ridwan Sakura 
Published: April 2019

The aim of this research was to analyze the needs on integrative content-based Indonesian language learning materials about agriculture for foreign speakers A2 level. Research and development design was used employing the Dick and Carey’s model combined with Borg and Gall’s model, as well as the model of learning materials development proposed by Tomlinson, Brown, and Jolly and Bolitho. This research was conducted at Indonesian for foreign speakers, Bogor Agricultural University program (BIPA IPB) on academic year 2015-2017. The procedure comprised: pre-study, students’ and teachers’ needs analysis on teaching materials at BIPA IPB. The findings indicated that the teaching materials about agriculture are not available yet at IPB. Besides, the needs on integrative content-based Indonesian language learning materials about agriculture for students at A2 level and the teachers were indicated high.
Non isolated coupled converter tied voltage source inverter drive
Authors: Kumar A. Suresh , Pongiannan R. K. , Bharatiraja C , Yusuff Adedayo , Yadaiah N 
Published: April 2019

<span>The voltage source inverters (VSI) are ever required section in the AC motor drive and power system interface. The electrical drive segment, the VSI based drives are unavoidable and they are closely operated with induction motor, permanent magnate synchronous motor and BLDC motor. These drives are normally needed high torque-power characters. Hence, the input DC-link side voltage is increased with help of increasing input AC in the rectifier input. However, this causes the power quality disturbance in the AC main and DC-link. In order to go for a increasing the AC voltage, the rectifier out is connected with DC to DC boost converter and they are increasing the DC voltage to meet out the drive DC-link voltage demand. With this aim, the paper proposes the idea to connect high step non-isolated high gain coupled DC to DC converter with three phase VSI for drives applications. The proposed converter has an ability to increase the voltage five times and the counter winding arrangement ratio of the converter is help for the further increase of gain. Inn this interface the front end DC to DC converters inductors are charged by making the short circuit with inverter switching. The converter voltage gain is controlled by shoot through of the VSI switch (converter gain directly proportional to inverter shoot through). The proposed converter has a higher degree of freedom in their values of winding and output voltage. Hence, the DC-link voltage of the inverter can be extended in any level. The operation principle and modes of the proposed DC to DC Source tied VSI is analyzed and simulated using MATLAB-Simulink software simulation. The laboratory based small scale power circuit is developed with help of control algorithm. The entire implementation is done through PIC microcontroller platform. The deign Investigation, system simulation and experimentation confirming the proposed DC to DC converter tied VSI drive system.</span>
Renewable power interface based rural telecom
Authors: Amutha W. Margaret , Andrew H. Caleb , Shajie A. Debie , Paulraj J. Praveen Immanuel 
Published: April 2019

<span>To power remote area telecom where grid supplied electricity is unreliable, an alternative exists in the form of renewable energy sources. These sources mainly rely on the weather condition of a particular area for their source of power. To overcome such problem, hybridization of energy sources are prefered. Hybridization of solar-wind systems use a separate converter for each source thus leading to a complex, bulky and less efficient system. This paper presents a single renewable power interface which allows solar and wind sources to supply the load separately or simultaneously depending on the availability of the energy sources. It uses cuk and sepic converter topologies to form cuk-seic interfece. These systems also use battery backup to store excess energy and to make best use of their operating characteristics. Performance comparison between a cuk-sepic renewable power interface with and without MPPT using MATLAB/SIMULINK is presented. A detailed componentwise analysis is also done to calculate best efficient interface. It is found that the cuk-sepic interface with MPPT is efficient. A lab level model for cuk-sepic interface is developed and tested. The experimental results proved the effective operation of a single renewable power cuk-sepic interface with MPPT. </span>
Flash Drought as Captured by Reanalysis Data: Disentangling the Contributions of Precipitation Deficit and Excess Evapotranspiration
Authors: Koster R. D. , Schubert S. D. , Wang H. , Mahanama S. P. , DeAngelis Anthony M. 
Published: April 2019

Abstract
Flash droughts—uncharacteristically rapid dryings of the land system—are naturally associated with extreme precipitation deficits. Such precipitation deficits, however, do not tell the whole story, for land surface drying can be exacerbated by anomalously high evapotranspiration (ET) rates driven by anomalously high temperatures (e.g., during heat waves), anomalously high incoming radiation (e.g., from reduced cloudiness), and other meteorological anomalies. In this study, the relative contributions of precipitation and ET anomalies to flash drought generation in the Northern Hemisphere are quantified through the analysis of diagnostic fields contained within the MERRA-2 reanalysis product. Unique to the approach is the explicit treatment of soil moisture impacts on ET through relationships diagnosed from the reanalysis data; under this treatment, an ET anomaly that is negative relative to the local long-term climatological mean is still considered positive in terms of its contribution to a flash drought if it is high for the concurrent value of soil moisture. Maps produced in the analysis show the fraction of flash drought production stemming specifically from ET anomalies and illustrate how ET anomalies for some droughts are related to temperature and radiation anomalies. While ET is found to have an important impact on flash drought production in the central United States and in parts of Russia known from past studies to be prone to heat wave–related drought, and while this impact does appear stronger during the onset (first several days) of flash droughts, overall the contribution of ET to these droughts is small relative to the contribution of precipitation deficit.
Acenocoumarol and Pentoxifylline in Intermittent Claudication. A Controlled Clinical Study
Authors: Dettori Anton G. , Pini Mario , Moratti Alfio , Paolicelli Mauro , Basevi Paolo , Quintavalla Roberto , Manotti Cesare , Di Lecce Carmelo 
Published: April 2019

The efficacy and safety of pentoxifylline (400 mg tid orally) and aceno coumarol, administered singly or in combination, in the treatment of intermit tent claudication associated with chronic occlusive arterial disease were evaluated in a multi-center, randomized, factorial, blind clinical trial involving 146 patients. The response to treatment was assessed by measuring pain-free walking time on the treadmill and by Doppler ankle/arm systolic pressure ratio at rest and after treadmill. Both pentoxifylline and acenocoumarol were significantly more effective than placebo in increasing the proportion of patients who improved their per formance on the treadmill after one year of treatment. Benefit from active treatment was also apparent from the results of Doppler examinations per formed after physical exercise. No significant differences were observed in comparing the effect of one ac tive drug versus the other or versus the combined treatment. Five major hemorrhagic complications were registered in anticoagulated pa tients, two fatal cerebral hemorrhages and one gastrointestinal bleeding occur ring in the group treated with both active drugs. The investigators conclude that (1) pentoxifylline is effective and safe in the treatment of patients with intermittent claudication (2) the benefits of oral anti coagulant therapy are outweighed by the risk of serious bleeding, and (3) the risk of bleeding is probably increased by the combined treatment with pentox ifylline.
Heparin and Prostacyclin
Authors: Camici M. , Evangelisti L. 
Published: April 2019

Hemodialysis performed with prostacyclin (5 ng/kg/min) as a sub stitute for heparin was studied in 10 patients. The subjects were studied during heparin perfusion alone and during heparin perfusion together with prostacyclin. The authors inves tigated the effect of two heparin regi mens (regimen I: 2,000 U/hr and reg imen II: 500 U/hr) upon plasma anti thrombin level (IU/mL) and activated thromboplastin time (sec). Our findings show: (1) prostacy clin can substitute for heparin anti coagulaton in hemodialysis; (2) the concomitant administration of pro stacyclin enhances the anticoagulant effect of heparin, based on the mea surement of the activated partial thromboplastin time; (3) the anti thrombin activity is increased by both treatments but more so with prostacyclin; and (4) platelet activa tion plays a role in limiting heparin anticoagulation, a conclusion partly supported by the finding that acti vated partial thromboplastin time is somewhat more prolonged by hepa rin when measured in platelet-poor rather than in platelet-rich plasma in the presence of prostacyclin. Physio pathologic implications of these pre liminary findings are discussed.
Study of the Antiischemic Action of EGb 761 in the Treatment of Peripheral Arterial Occlusive Disease by TcPo2 Determination
Authors: Mouren X. , Caillard Ph. , Schwartz F. 
Published: April 2019

In a randomized, placebo-controlled, double-blind, parallel study of 20 patients, the anti ischemic effect of EGb 761 (Ginkgo biloba Extract) was studied by measuring the tran scutaneous partial pressure of oxygen (TcPo2) during exercise. Transcutaneous oximetry during exercise provides a good, noninvasive estimation of local arterial perfusion and constitutes a real index of local and regional capillary perfusion. Twenty patients between the ages of forty-four and seventy-three years suffering from claudicating atherosclerotic arterial occlusive disease in stage II according to the Leriche and Fontaine classification, diagnosed for more than a year and stable for three months, were included. The eligible patients received placebo for fifteen days under single-blind conditions. At the end of this preinclusion period, the eligibility criteria were checked and the patients were randomized to two treatment groups. The first group received 320 mg per day of EGb 761 for four weeks and the second group received placebo. The treadmill walking test was performed under standardized conditions at the same time of day and by the same investigator. In a comparison of the differences before and after treatment, the areas of ischemia decreased by 38% in the EGb 761 group but remained essentially stable (+5%) in the placebo group. This difference between groups is significant (F [1.18] =4.91; P=0.04) and the 95% confidence interval for the difference ranges from 0.89 to 3.87. This study confirmed significantly the rapid antiischemic action of EGb 761 and its value in the management of peripheral arterial occlusive disease at the stage of inter mittent claudication.
The pH Value as a Factor Modifying Bacterial Colonization of Sinonasal Mucosa in Healthy Persons
Authors: Morawska-Kochman Monika , Jermakow Katarzyna , Nelke Kamil , Zub Krzysztof , Pawlak Wojciech , Dudek Krzysztof , Bochnia Marek 
Published: April 2019

Objective: The aim of the present study is to determine and compare the range of pH value in nasal and sinus cavities in vivo regarding the presence of bacteria colonizing sinonasal mucosa among healthy subjects. Methods: The nasal pH value measurement using a portable pH meter (Dx-pH System, Restech) and the microbiological culture swab were taken from beneath the middle turbinate and in the sinus cavity in 39 healthy subjects during maxillary bone corrective osteotomy with the Le Fort I technique. Results: The mean pH value (independently of sex, P = .441) in the healthy sinus cavity was statistically higher than in the nasal middle meatus: 7.96 (SD ± 0.29) versus 7.83 (SD ± 0.30) ( P = .032). Forty-eight strains of bacteria were cultured from sinus maxillaries cavities—aerobic 36.8%, aerobic and anaerobic 52.6%, anaerobic only 10.5%—and 23 strains from the nasal meatus—aerobic 25%, aerobic and anaerobic 75%. A statistically significant correlation was found between the type and location of 8 microorganisms, especially Propionibacterium acnes, identified only in the sinus cavities. Conclusions: Differences in the pH value between the middle nasal meatus and the maxillary sinus are characteristic of healthy subjects and could be associated with the diverse bacterial flora. The role of bacteria Propionibacterium acnes seems to be crucial for the pH range and sinus flora in healthy subjects.
Clinical Presentation and Outcome in Autoimmune Encephalitis Associated With N-Type Voltage-Gated Calcium Channels in Children
Authors: Kornitzer Jeffrey , Taha Firas , Segal Eric 
Published: April 2019

Objective: We present the diagnostic and clinical course of the first multicenter case series of pediatric patients with autoimmune encephalitis associated with N-type voltage-gated calcium channel antibodies. Methods: Data from 2 university hospitals were retrospectively reviewed and records of 3 patients with autoimmune encephalitis associated with N-type voltage-gated calcium channel antibodies were evaluated. Results: The 3 pediatric patients (all female) had symptoms that spanned the clinical spectrum. All 3, however, had regression of expressive language and agitation. Neuroimaging in all 3 patients was normal; electroencephalographic (EEG) findings varied among the 3 patients. Positive titers against the N-type voltage-gated calcium channel antibody were found in their cerebrospinal fluid. Following administration of intravenous immunoglobulin, all 3 had improvement in their core presenting symptoms. Conclusion: Autoimmune encephalitis associated with N-type voltage-gated calcium channel antibodies in the pediatric population presents with a wide clinical spectrum, although expressive language delay and agitation seem to be common symptoms. Treatment with intravenous immunoglobulin improves core symptoms.
Everyday ethical challenges of nurse-physician collaboration
Authors: Sabone Motshedisi , Mazonde Pelonomi , Cainelli Francesca , Maitshoko Maseba , Joseph Renatha , Shayo Judith , Morris Baraka , Muecke Marjorie , Wall Barbra Mann , Hoke Linda , Peng Lilian , Mooney-Doyle Kim , Ulrich Connie M 
Published: April 2019

Background: Collaboration between physicians and nurses is key to improving patient care. We know very little about collaboration and interdisciplinary practice in African healthcare settings. Research question/aim: The purpose of this study was to explore the ethical challenges of interdisciplinary collaboration in clinical practice and education in Botswana Participants and research context: This qualitative descriptive study was conducted with 39 participants (20 physicians and 19 nurses) who participated in semi-structured interviews at public hospitals purposely selected to represent the three levels of hospitals in Botswana (referral, district, and primary). Ethical considerations: Following Institutional Review Board Approval at the University of Pennsylvania and the Ministry of Health in Botswana, participants’ written informed consent was obtained. Findings: Respondents’ ages ranged from 23 to 60 years, and their duration of work experience ranged from 0.5 to 32 years. Major qualitative themes that emerged from the data centered on the nature of the work environment, values regarding nurse–doctor collaboration, the nature of such collaboration, resources available for supporting collaboration and the smooth flow of work, and participants’ views about how their work experiences could be improved. Discussion: Participants expressed concerns that their work environment compromised their ability to provide high-quality and safe care to their patients. The physician staffing structure was described as consisting of a few specialists at the top, a vacuum in the middle that should be occupied by senior doctors, and junior doctors at the bottom—and not a sufficient number of nursing staff. Conclusion: Collaboration between physicians and nurses is critical to optimizing patients’ health outcomes. This is true not only in the United States but also in developing countries, such as Botswana, where health care professionals reported that their ethical challenges arose from resource shortages, differing professional attitudes, and a stressful work environment.
A Simple Method of Measuring the Distance From the Schöttle Point to the Medial Distal Femoral Physis With MRI
Authors: Bishop Meghan E. , Black Sheena R. , Nguyen Joseph , Mintz Douglas , Stein Beth Shubin 
Published: April 2019

Background: Medial patellofemoral ligament (MPFL) reconstruction is the treatment of choice for recurrent patellar instability in the skeletally immature patient. Avoiding the open physes during anatomic MPFL reconstruction is a challenge in this population. Purpose: To describe a novel method using magnetic resonance imaging (MRI) to determine the distance from the Schöttle point to the medial distal femoral physis among skeletally immature individuals with patellar instability. Study Design: Descriptive laboratory study. Methods: Preoperative MRI scans were analyzed from 34 patients with open distal femoral physes and lateral patellar instability. With the multiplanar reconstruction mode on a picture archiving and communication system (PACS), the location of the Schöttle point was determined according to previously reported distances from the posterior femoral cortical line and the posterior origin of the medial femoral condyle. This location was then extrapolated to the most medial sagittal slice on MRI showing the medial distal femoral physis. The distance was measured from this point to the most distal aspect of the physis. Results: The mean age of the study cohort was 13.6 years (range, 10.6-15.7 years); there were 13 males and 21 females. The mean distance from the medial distal femoral physis to the Schöttle point was 7.27 ± 1.78 mm. The Schöttle point was distal to the medial distal femoral physis in all cases. There was no significant correlation between age and mean distance in either the overall study population ( r = 0.046, P = .798) or when stratified by sex (females, P = .629; males, P = .089). The distance between the Schöttle point and the medial distal femoral physis was shorter for females than for males (6.51 vs 7.71 mm, P = .043). After adjustment for age, females on average were 1.31 mm closer to the Schöttle point than were males ( B = –1.31, P = .041). Conclusion: This technique can be used to determine the distance between the medial distal femoral physis and the Schöttle point. The Schöttle point was distal to the physis in all patients, and it was closer to the physis in skeletally immature females compared with age-matched males. Clinical Relevance: The long-term repercussions of improperly placed MPFL reconstruction include recurrent patellar instability, increased patellofemoral contact pressures and overtensioning of the ligament, and possibly patellofemoral arthritis. The current technique can be used preoperatively to determine the appropriate safe distance for drilling a socket distal to the physis.