bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deletion of Tet proteins results in quantitative disparities during ESC differentiation partially attributable to differences in gene expression
Authors: Reimer Michael J , Pulakanti Kirthi , Shi Linzheng , Abel Alex , Liang Mingyu , Malarkannan Subramaniam , Rao Sridhar 
Published: April 2019

Abstract
Background: The Tet protein family (Tet1, Tet2, and Tet3) regulate DNA methylation through conversion of 5-methylcytosine to 5-hydroxymethylcytosine which can ultimately result in DNA demethylation and play a critical role during early mammalian development and pluripotency¬. While multiple groups have generated knockouts combining loss of different Tet proteins in murine embryonic stem cells (ESCs), differences in genetic background and approaches has made it difficult to directly compare results and discern the direct mechanism by which Tet proteins regulate the transcriptome. To address this concern, we utilized genomic editing in an isogenic pluripotent background which permitted a quantitative, flow-cytometry based measurement of pluripotency in combination with genome-wide assessment of gene expression and DNA methylation changes. Our ultimate goal was to generate a resource of large-scale datasets to permit hypothesis-generating experiments.
Results: We demonstrate a quantitative disparity in the differentiation ability among Tet protein deletions, with Tet2 single knockout exhibiting the most severe defect, while loss of Tet1 ¬alone or combinations of Tet genes showed a quantitatively intermediate phenotype. Using a combination of transcriptomic and epigenomic approaches we demonstrate an increase in DNA hypermethylation and a divergence of transcriptional profiles in pluripotency among Tet deletions, with loss of Tet2 having the most profound effect in undifferentiated ESCs.
Conclusions: We conclude that loss of Tet2 has the most dramatic effect both on the phenotype of ESCs and the transcriptome compared to other genotypes. While loss of Tet proteins increased DNA hypermethylation, especially in gene promoters, these changes in DNA methylation did not correlate with gene expression changes. Thus, while loss of different Tet proteins alters DNA methylation, this change does not appear to be directly responsible for transcriptome changes. Thus, loss of Tet proteins likely regulates the transcriptome epigenetically both through altering 5mC but also through additional mechanisms. Nonetheless, the transcriptome changes in pluripotent Tet2-/- ESCs compared to wild-type implies that the disparities in differentiation can be partially attributed to baseline alterations in gene expression.
Resistant Pepper Carrying N, Me1, and Me3 have Different Effects on Penetration and Reproduction of Four Major Meloidogyne species
Authors: Hajihassani Abolfazl , Rutter William B. , Luo Xuelin 
Published: April 2019

Abstract
Root-knot nematode (Meloidogyne spp.) exhibits a substantial problem in pepper production, causing reduction in yield throughout the world. Continued assessment for root-knot resistance is important for developing new resistance cultivars. In this study, the effect of Me and N genes on the penetration and reproduction of M. incognita race 3, M. arenaria race 1, M. javanica, and M. haplanaria was examined under controlled greenhouse conditions using susceptible and resistant pepper lines/cultivars (Mellow Star, Yolo Wonder B, Charleston Belle, HDA-149, HDA-330, PM-217, and PM-687) differing in the presence or absence of resistant genes. The penetration and resistance responses of these pepper lines differed depending on the nematode species. More second-stage juveniles penetrated roots of susceptible control cultivar Mellow Star than roots of resistant cultivars/lines. Although, there was no significant difference in the nematode penetration among resistant lines 1 and 3 days after inoculation (DAI), variability in the penetration of M. incognita, M. javanica, and M. haplanaria was observed 5 DAI. This demonstrates the variability among different nematode resistance genes to invasion by Meloidogyne spp. Based on nematode gall index (GI) and reproduction factor (RF), Charleston Belle, HDA-149, PM-217 and PM-687 showed very high resistance (GI < 1 and RF < 0.1) to M. incognita, M. arenaria, and M. javanica. Although, all the Meloidogyne-resistant pepper lines evaluated were resistant to M. javanica and M. haplanaria, the susceptible cultivar Mellow Star was a good host for all nematode species having an RF ranging from 8.1 to 34.7. The N, Me1, and Me3 genes controlled resistance to reproduction of all species of Meloidogyne examined.
The quench control of water estimates in convergent margin magmas
Authors: Gavrilenko Maxim , Krawczynski Michael , Ruprecht Philipp , Li Wenlu , Catalano Jeffrey G. 
Published: April 2019

AbstractHere we present a study on the quenchability of hydrous mafic melts. We show via hydrothermal experiments that the ability to quench a mafic hydrous melt to a homogeneous glass at cooling rates relevant to natural samples has a limit of no more than 9 ± 1 wt% of dissolved H2O in the melt. We performed supra-liquidus experiments on a mafic starting composition at 1–1.5 GPa spanning H2O-undersaturated to H2O-saturated conditions (from ~1 to ~21 wt%). After dissolving H2O and equilibrating, the hydrous mafic melt experiments were quenched. Quenching rates of 20 to 90 K/s at the glass transition temperature were achieved, and some experiments were allowed to decompress from thermal contraction while others were held at an isobaric condition during quench. We found that quenching of a hydrous melt to a homogeneous glass at quench rates comparable to natural conditions is possible at water contents up to 6 wt%. Melts containing 6–9 wt% of H2O are partially quenched to a glass, and always contain significant fractions of quench crystals and glass alteration/devitrification products. Experiments with water contents greater than 9 wt% have no optically clear glass after quench and result in fine-grained mixtures of alteration/devitrification products (minerals and amorphous materials). Our limit of 9 ± 1 wt% agrees well with the maximum of dissolved H2O contents found in natural glassy melt inclusions (8.5 wt% H2O). Other techniques for estimating pre-eruptive dissolved H2O content using petrologic and geochemical modeling have been used to argue that some arc magmas are as hydrous as 16 wt% H2O. Thus, our results raise the question of whether the observed record of glassy melt inclusions has an upper limit that is partially controlled by the quenching process. This potentially leads to underestimating the maximum amount of H2O recycled at arcs when results from glassy melt inclusions are predominantly used to estimate water fluxes from the mantle.
Effect of bubble characteristics and nozzle size on the membrane distillation enhanced by gas–liquid two-phase flow
Authors: Zhang Dashuai , Zhang Xiaopeng , Chen Li , Lili Wang , Di Wu , Xiong Yuqin , Lin Qiang , Shi Zaifeng 
Published: April 2019

Abstract
This study investigates the membrane performance and fouling control in bubble-assisted sweeping gas membrane distillation with high concentration saline (333 K saturated solution) as feed. The results show that a longer bubbling interval (3 min) at a fixed bubbling duration of 30 s can most efficiently increase the flux enhancement ratio up to 1.518. Next, the flux increases with the gas flow rate under a relatively lower level, but tends to plateau after the threshold level (1.2 L·min−1). Compared to the non-bubbling case, the permeate flux reaches up to 1.623-fold at a higher bubble relative humidity of 80%. It was also found that greater flux enhancement can be achieved and, meanwhile, dramatic flux decline can be delayed for an intermittent bubbling system with a smaller nozzle size. These results accord well with the observations of fouling deposition in situ on the membrane surface with scanning electron microscope (SEM).
Analysis of Spatial Distribution Pattern of Reservoir Petrophysical Properties for Horizontal Well Performance Evaluation-A Case Study of Reservoir X
Authors: Abraham Aidoo Borsah , Evans Annan Boah , Thompson Brantson Eric 
Published: April 2019

Introduction:Building a large number of static models to analyze reservoir performance is vital in reservoir development planning. For the purpose of maximizing oil recovery, reservoir behavior must be modelled properly to predict its performance. This requires the study of the variation of the reservoir petrophysical properties as a function of spatial location.Methods:In recent times, the method used to analyze reservoir behavior is the use of reservoir simulation. Hence, this study seeks to analyze the spatial distribution pattern of reservoir petrophysical properties such as porosity, permeability, thickness, saturation and ascertain its effect on cumulative oil production. Geostatistical techniques were used to distribute the petrophysical properties in building a 2D static model of the reservoir and construction of dynamic model to analyze reservoir performance. Vertical to horizontal permeability anisotropy ratio affects horizontal wells drilled in the 2D static reservoir. The performance of the horizontal wells appeared to be increasing steadily as kv/kh increases. At kv/kh value of 0.55, a higher cumulative oil production was observed compared to a kv/kh ratio of 0.4, 0.2, and 0.1. In addition, horizontal well length significantly affects cumulative oil production of the petroleum reservoir studied.Results:At kv/kh of 0.55, the results of the analysis showed a rapid decrement in cumulative oil production as the horizontal well length decreases. Considering horizontal well length of 3000 ft, 2000 ft, and 1500 ft, a minimum cumulative oil production was obtained from a horizontal well length of 1500 ft.Conclusion:The geostatistical and reservoir simulation methods employed in this study will serve as an insight in analyzing horizontal well performance.
Pelaksanaan Fungsi Hubungan Masyarakat dan Publisitas pada Organisasi Non-Profit
Authors: Wisataone Voettie 
Published: April 2019

Dewasa ini, tidak hanya organisasi profit yang menjalankan berbagai macam strategi pemasaran untuk menjaring konsumen. Organisasi non-profit pun juga melakukan berbagai strategi pemasaran agar tetap eksis, sehingga dapat mendatangkan sejumlah donatur. Salah satu strategi pemasaran yang digunakan adalah dengan menjalankan fungsi hubungan masyarakat (humas) dan publisitas. Pos Keadilan Peduli Umat (PKPU) menjalankan fungsi humas dan publisitas, meskipun secara struktur tidak memiliki devisi humas. Peran fungsi humas dan publisitas dijalankan oleh bidang kemitraan. Hasil wawancara dan observasi menunjukkan bahwa fungsi humas dan publisitas yang dijalankan oleh PKPU Yogyakarta, antara lain; persconference, publikasi melalui media sosial, dan bekerjasama dengan media radio. Sasaran dari program tersebut adalah para donatur dan masyarakat secara luas. Tujuannya adalah agar dapat membangun citra yang baik, sehingga donatur dan masyarakat dapat berdonasi melalui PKPU Yogyakarta. Kata kunci : Fungsi Humas dan Publisitas, PKPU Yogyakarta, Organisasi Non-Profit Abstract: Implementation of Public Relations and Publicity Functions in Non-Profit Organizations. Today, not only profit organizations run various kinds of marketing strategies to attract consumers. Non-profit organizations also carry out various marketing strategies in order to continue to exist, so that they can bring in a number of donors. One of the marketing strategies used is to carry out public relations and publicity functions. The Community Care Justice Post (PKPU) carries out public relations and publicity functions, even though structurally there is no public relations division. The role of public relations and publicity functions is carried out by the partnership sector. The results of interviews and observations indicate that the functions of public relations and publicity run by PKPU Yogyakarta, among others; persconference, publication through social media, and collaborating with radio media. The objectives of the program are donors and the community at large. The aim is to build a good image, so donors and the community can donate through PKPU Yogyakarta. Keywords: Function of Public Relations and Publicity, PKPU Yogyakarta, Non-Profit Organizations
Peran E-Servqual Dalam Customer Engagement
Authors: Wijayanti Nadia Sasmita 
Published: April 2019

Sejumlah brand sukses menempatkan diri di hati konsumen, namun banyak pula yang gagal. Keberhasilan brand memikat hati customer berarti sukses menjalin hubungan dengan customer. Perlu cara dan startegi yang tepat agar brand mampu memikat kosumen dan keluar sebagai market leader. Dalam menjalankan bisnis dan memberikan pelayanan konsumen perlu memperhatikan keinginan konsumen. Di era serba modern dan informasi tanpa batas, bermunculan marketplace dan perusahaan eksponensial, menyebabkan keinginan konsumen untuk dilayani serba cepat dan ringkas. Kualitas jasa layanan online yang maksimal akan menciptakan customer loyalty yang berujung pada customer engagement. Kata kunci: E-servqual, Kualitas layanan jasa online, customer loyalty, customer engagement. Abstract: The Role of E-Servqual in Customer Engagement. A number of brands successfully place themselves in the hearts of consumers, but many also fail. The success of the brand captivates the customer means successful relationship with the customer. Need the right way and strategy so that the brand can attract consumers and come out as the market leader. In running a business and providing customer service, it is necessary to pay attention to consumer desires. In the modern era and unlimited information, emerging marketplaces and exponential companies, cause consumers to be served quickly and concisely. The maximum quality of online services will create customer loyalty which leads to customer engagement. Kata kunci: E-servqual, Online Service Quality, customer loyalty, customer engagement.
Peran Teknologi untuk Pengembangan Karir Sekretaris
Authors: Rosidah Rosidah , Arantika Tina 
Published: April 2019

Pekerjaan Sekretaris dalam kantor membutuhkan media teknologi informasi. Dengan menggunakan bantuan teknologi tersebut pekerjaan kantor dapat efisien dan efektif. Hal tersebut menjadikan kerja sekretaris lebih produktif. Karir sekretaris dapat dibangun melalui peningkatakn ketrampilan teknologi informasi maupun pengembangan soft skill. Perpaduan tersebut akan lebih mengeksiskan peran sekretaris dalam memberikan kontribusinya pada kemajuan pimpinan maupun perusahaan. Dalam tulisan ini akan dikupas hal-hal penting kemampuan teknologi informasi bagi sekretaris serta kiat mengembangkan karir sekretaris Kata kunci: Teknologi Informasi, Sekretaris, Karir Sekretaris Abstract: Role of Technology for Secretary Career Development. Secretary's work in the office requires information technology media. By using technology assistance, office work can be efficient and effective. This makes the work of the secretary more productive. Secretarial careers can be built through enhancing information technology skills and developing soft skills. This combination will further exert the role of secretary in contributing to the progress of the leadership and the company. In this paper, important information technology capabilities for secretaries and tips on developing a career for the secretary will be discussed. Keyword: Information Technology, Secretary, Career Secretary
Integrasi bahasa Inggris dalam Proses Pembelajaran
Authors: Kusuma Chusnu Syarifa Diah 
Published: April 2019

Salah satu bahasa yang paling luas dan penting di dunia adalah bahasa Inggris. Mulai dari konferensi akademik internasional hingga laporan berita bahkan lirik musik popular menggunakan bahasa Inggris. Tidak hanya itu bahasa Inggris digunakan untuk menyampaikan informasi dan berita serta menjadi bahasa bisnis dan pemerintahan. Bahasa Inggris diperlukan dalam diplomasi dan berperan dalam profesi. Bahasa Inggris adalah bahasa internasional. Program pendidikan berkualitas tinggipun membutuhkan kefasihan bahasa Inggris. Belajar bahasa Inggris adalah penting. Oleh karena itu pendidik harus siap mengembangkan kompetensi bahasa Inggris sebagai bahasa internasional kepada siswa melalui kegiatan proses pembelajaran. Kata kunci : Bahasa Inggris, pembelajaran Abstract : Integration of English in the Learning Process. One of the most extensive and important languages in the world is English. Starting from international academic conferences to news reports and even popular music lyrics using English. Not only that English is used to convey information and news and to become the language of business and government. English is needed in diplomacy and plays a role in the profession. English is the international language. High quality education programs also require English fluency. Learning English is important. Therefore educators must be prepared to develop English language competence as an international language to students through the learning process activities Keywords: English, learning
Perkembangan Surat Niaga di Era Distrupsi (Studi Kasus Di Perusahaan Jasa PT JNE)
Authors: Kuswantoro Agung , Aini Kuatina Nur 
Published: April 2019

Tujuan penelitian ini adalah untuk mengetahui surat-surat niaga yang masih digunakan di era disrupsi, dalam berkomunikasi dengan supplier dan konsumen. Metode penelitiannya adalah kualitatif deskriptif. Setting penelitian dalam penelitian ini yaitu perusahaan jasa Agen JNE Menoreh, Semarang. Subjek penelitian ini adalah pegawai JNE. Teknik pengumpulan data dilakukan dengan wawancara dan observasi. Hasil penelitiannya adalah surat-surat niaga yang masih digunakan di era disrupsi pada JNE dari ke-13 surat niaga, hanya ada 2 jenis surat yang digunakan secara manual yaitu surat tagihan dan surat klaim. Ada 11 surat yang tidak dipakai di JNE yaitu surat perkenalan, surat pesanan, surat balasan klaim, surat permintaan penawaran, surat perjanjian kerjasama, surat pengiriman barang, surat balasan pesanan, surat penawaran, surat adjustment, surat konfirmasi pesanan, dan surat penangguhan pembayaran. Surat-surat niaga yang sudah tidak digunakan lagi dalam JNE digantikan menggunakan bukti-bukti trasaksi dari aplikasi media elektronik dan aplikasi. Saran dalam penelitian ini, bahwa di era disrupsi diharapkan dalam menggunakan fasilitas teknologi komunikasi dalam korespondensi, karena melalui teknologi komunikasi dapat mempermudah dalam pekerjaannya. Kata kunci: Surat Niaga, Era Disrupsi, JNE Abstract: Development of Commerce in the Distorted Era (Case Study in PT JNE Service Company). This study aims at knowing business letters which are still used and applicable at the disruption era to communiate with suppliers and customers. The design of study was qualitative descriptive. Further, the study was conducted in JNE agent, Menoreh Semarang with its staff as the subject. To collect the data, interview and observation were employed. Then, the finding show that there only 2 (two) of 13 business letters which are still applicable and written manually nowdays. Those two letters are bills and claim letter. The JNE agent has dropped 11 business letters; they are sales letter inquiry letter, order letter, reply to inquiry, reply to order, dispatch letter, complaint letter, adjusment letter, MoU, letter for confirming the payment, letter of payment deferral. JNE has implemented online system for replacing most of manual business letters. The evidences from the online transaction are used instead of the manual ones. Finally, in this disruption era, up to date technology and information such as online application and system should be implemented by businessmen as they can be easier and efficier in doing the business Keyword: Business Letters, Disruption Era, JNE
Evaluasi Program Pelatihan Keterampilan Dasar Komunikasi Bagi Siswa di SMKN 1 Godean Sleman
Authors: Aw Suranto , Widiarti Pratiwi Wahyu , Hastasari Chatia 
Published: April 2019

. Studi evaluasi ini bertujuan untuk mengungkap keefektifan program pelatihan keterampilan dasar komunikasi bagi siswa Kelas XI Kompetensi Keahlian Administrasi Perkantoran SMKN 1 Godean Sleman. Penelitian ini adalah penelitian evaluasi dengan menggunakan model evaluasi program pelatihan dari Kirkpatrick yang mencakup empat tahap: reaction, learning, behavior, result. Pendekatan yang digunakan adalah kualitatif. Subjek penelitian adalah peserta pelatihan dan pendampingan keterampilan dasar komunikasi. Pengumpulan data menggunakan teknik wawancara dan pengamatan. Analisis data menggunakan teknik interaktif dari Miles dan Huberman. Hasil penelitian mengungkap: (1) Reaction : tanggapan peserta terhadap pelatihan sangat positif, karena mendukung kompetensi siswa; (2) Learning: menunjukkan peserta memperoleh sumber belajar dan pengalaman dari pelatihan; (3) Behavior: adanya perubahan keterampilan dalam berkomunikasi; (4) Result: meningkatnya keterampilan berkomunikasi dalam kehidupan sehari-hari. Kata kunci: Evaluasi, Pelatihan, Keterampilan Dasar Komunikasi, Siswa Abstract: AN EVALUATION OF COMMUNICATION BASIC SKILLS TRAINING PROGRAM AT SMK N 1 GODEAN SLEMAN. This evaluation research aims to investigate the effectiveness of the basic communication skills training program for students of grade XI of Office Administration Skills Competency at SMK 1 Godean Sleman. This research is evaluation research employing Kirkpatrick's training program evaluation model which includes four stages, namely: reaction, learning, behavior, and results. This research utilizes a qualitative approach. The research subjects include participants of training and mentoring of basic communication skills. Data collection uses interview and observation techniques. Data were analyzed using an interactive technique of Miles and Huberman. The results of the research are: (1) Reaction: participants' responses to training were very positive because the training could enhance their competence; (2) Learning: participants get learning resources and experience from training; (3) Behavior: a change regarding the participants communication skills occur; (4) Results: students communication skills in everyday life increases. Keyword: evaluation, training, basic communication skills, students.