bluuebunny/crossref_metadata_2025_split huggingface dataset:
Formulation, characterization and evaluation of mRNA-loaded dissolvable polymeric microneedles (RNApatch)
Authors: Koh Kai Jun , Liu Yi , Lim Seng Han , Loh Xian Jun , Kang Lifeng , Lim Chee Yen , Phua Kyle K. L. 
Published: August 2018

AbstractIn this paper, we report a proof of concept study on the fabrication, characterization and therapeutic evaluation of in vitro transcribed messenger RNA (mRNA) loaded in a dissolving microneedle patch (RNApatch). We show that low molecular weight polyvinylpyrrolidone (PVP) can directly be used without further purification for RNApatch fabrication with no detectable mRNA degradation. Physical and functional integrity of mRNA stored within the RNApatch are completely preserved for at least 2 weeks under ambient conditions. While the loading of mRNA into RNApatch is limited by the solubility of mRNA in concentrated PVP solution, mechanical strength of RNApatch is not compromised by the presence of mRNA. RNApatch can mediate in vivo transgene expression of mRNA encoding luciferase for up to 72 hours and transfection efficiency and kinetics mediated by RNApatch compares favorably to subcutaneous injection. Interestingly, mRNA transfection efficiency does not correlate with contact surface area but instead increases with deeper delivery depths. In an E.G7-OVA immunotherapy model, RNApatch induces slightly higher cellular and humoral immune responses compared to subcutaneous injection. In conclusion, RNApatch is a viable delivery platform for mRNA and represents an attractive option with significant translation potential for the delivery of mRNA therapeutics.
Instability-Induced Pattern Transformation in Soft Metamaterial with Hexagonal Networks for Tunable Wave Propagation
Authors: Gao Chao , Slesarenko Viacheslav , Boyce Mary C. , Rudykh Stephan , Li Yaning 
Published: August 2018

AbstractInstability-induced pattern transformations of the architectured multi-phase soft metamaterial under bi-axial compression were explored. The soft metamaterial is composed of two phases: a soft matrix and a reinforcing hexagonal network embedded in the matrix. Equi-biaxial loading is found to induce both micro- and macro- instabilities in the networked architecture. Two types of instability patterns were observed, dependent upon the architecture geometry and the material combination. The critical strain for triggering instability and the two resulting types of patterns was derived, and a theoretical criterion for the transition between the two patterns was determined. Type I patterns retain the original periodicity of the architecture but wrinkles the network walls whereas Type II patterns transform the overall periodicity of the architecture while bending the network walls. Elastic wave propagation analysis was performed for the two distinct patterns under both stressed and stress-free conditions: a change in band gaps is found for both instability-induced pattern transformations, but differs for each type due to their dramatic difference in structure transformation (i.e. Type I wall wrinkling vs. Type II periodicity switching). The distinguished mechanical behavior and the rich properties of this category of multi-phase soft metamaterial can be used to design new smart materials with switchable functionalities controllable by deformation.
The role of lipids in the inception, maintenance and complications of dengue virus infection
Authors: Melo Carlos Fernando Odir Rodrigues , Delafiori Jeany , Dabaja Mohamad Ziad , de Oliveira Diogo Noin , Guerreiro Tatiane Melina , Colombo Tatiana Elias , Nogueira Maurício Lacerda , Proenca-Modena Jose Luiz , Catharino Rodrigo Ramos 
Published: August 2018

AbstractDengue fever is a viral condition that has become a recurrent issue for public health in tropical countries, common endemic areas. Although viral structure and composition have been widely studied, the infection phenotype in terms of small molecules remains poorly established. This contribution providing a comprehensive overview of the metabolic implications of the virus-host interaction using a lipidomic-based approach through direct-infusion high-resolution mass spectrometry. Our results provide further evidence that lipids are part of both the immune response upon Dengue virus infection and viral infection maintenance mechanism in the organism. Furthermore, the species described herein provide evidence that such lipids may be part of the mechanism that leads to blood-related complications such as hemorrhagic fever, the severe form of the disease.
A universal bioluminescence resonance energy transfer sensor design enables high-sensitivity screening of GPCR activation dynamics
Authors: Schihada Hannes , Vandenabeele Sylvie , Zabel Ulrike , Frank Monika , Lohse Martin J. , Maiellaro Isabella 
Published: August 2018

AbstractG-protein-coupled receptors (GPCRs) represent one of the most important classes of drug targets. The discovery of new GCPR therapeutics would greatly benefit from the development of a generalizable high-throughput assay to directly monitor their activation or de-activation. Here we screened a variety of labels inserted into the third intracellular loop and the C-terminus of the α2A-adrenergic receptor and used fluorescence (FRET) and bioluminescence resonance energy transfer (BRET) to monitor ligand-binding and activation dynamics. We then developed a universal intramolecular BRET receptor sensor design to quantify efficacy and potency of GPCR ligands in intact cells and real time. We demonstrate the transferability of the sensor design by cloning β2-adrenergic and PTH1-receptor BRET sensors and monitored their efficacy and potency. For all biosensors, the Z factors were well above 0.5 showing the suitability of such design for microtiter plate assays. This technology will aid the identification of novel types of GPCR ligands.
Effects of Specific Language Impairment on a Contrastive Dialect Structure: The Case of Infinitival TO Across Various Nonmainstream Dialects of English
Authors: Rivière Andrew M. , Oetting Janna B. , Roy Joseph 
Published: August 2018

PurposeUsing data from children who spoke various nonmainstream dialects of English and who were classified as either children with specific language impairment (SLI) or typically developing (TD) children, we examined children's marking of infinitival TO by their dialect and clinical status.MethodThe data came from 180 kindergartners (91 speakers of African American English, 60 speakers of Southern White English, 29 speakers of +Cajun); 53 were children with SLI, and 127 were TD children. Data included 4,537 infinitival TO contexts extracted from language samples; each was coded as zero or overtly marked and by preceding verb context (i.e., verbs of motion vs. other).ResultsAcross dialects, overall rates of zero marking differed by the children's clinical status (SLI > TD), and other verb contexts accounted for this result. Across the TD and SLI groups, dialect variation was evident for verbs of motion contexts, and the effect was stronger for the TD than for the SLI groups, particularly if the TD children's dialects were classified as +Cajun.ConclusionChildren's marking of infinitival TO can be affected by both their dialect and clinical status. Results support language assessments that include context-specific rate-based measures of infinitival TO and other contrastive structures when they prove useful for understanding the linguistic profile of SLI within a dialect.
Executive Function in Deaf Children: Auditory Access and Language Access
Authors: Hall Matthew L. , Eigsti Inge-Marie , Bortfeld Heather , Lillo-Martin Diane 
Published: August 2018

Purpose
Deaf children are frequently reported to be at risk for difficulties in executive function (EF); however, the literature is divided over whether these difficulties are the result of deafness itself or of delays/deficits in language that often co-occur with deafness. The purpose of this study is to discriminate these hypotheses by assessing EF in populations where the 2 accounts make contrasting predictions.

Method

We use a between-groups design involving 116 children, ages 5–12 years, across 3 groups: (a) participants with normal hearing (
n
= 45), (b) deaf native signers who had access to American Sign Language from birth (
n
= 45), and (c) oral cochlear implant users who did not have full access to language prior to cochlear implantation (
n
= 26). Measures include both parent report and performance-based assessments of EF.

Results
Parent report results suggest that early access to language has a stronger impact on EF than early access to sound. Performance-based results trended in a similar direction, but no between-group differences were significant.

Conclusions
These results indicate that healthy EF skills do not require audition and therefore that difficulties in this domain do not result primarily from a lack of auditory experience. Instead, results are consistent with the hypothesis that language proficiency, whether in sign or speech, is crucial for the development of healthy EF. Further research is needed to test whether sign language proficiency also confers benefits to deaf children from hearing families.
Room temperature continuous wave operation of InAs/AlSb‐based quantum cascade laser at λ ∼11 µm
Authors: Loghmari Z. , Bahriz M. , Thomas D. Díaz , Meguekam A. , Van H. Nguyen , Teissier R. , Baranov A.N. 
Published: August 2018

The InAs/AlSb quantum cascade lasers (QCLs) operating ∼11 µm in the continuous wave (cw) regime at room temperature are reported. The lasers with cleaved Fabry–Perot resonators demonstrated pulsed threshold current densities below 1 kA/cm2 at 300 K and operated in a cw up to 320 K that is the maximum temperature of such regime of operation for InAs‐based QCLs. Distributed feedback lasers fabricated from the same wafer exhibited single frequency emission near 11 µm and operated in the cw regime up to 295 K.
The impact crater at the origin of the Julia family detected with VLT/SPHERE?
Authors: Vernazza P. , Brož M. , Drouard A. , Hanuš J. , Viikinkoski M. , Marsset M. , Jorda L. , Fetick R. , Carry B. , Marchis F. , Birlan M. , Fusco T. , Santana-Ros T. , Podlewska-Gaca E. , Jehin E. , Ferrais M. , Bartczak P. , Dudziński G. , Berthier J. , Castillo-Rogez J. , Cipriani F. , Colas F. , Dumas C. , Ďurech J. , Kaasalainen M. , Kryszczynska A. , Lamy P. , Le Coroller H. , Marciniak A. , Michalowski T. , Michel P. , Pajuelo M. , Tanga P. , Vachier F. , Vigan A. , Warner B. , Witasse O. , Yang B. , Asphaug E. , Richardson D. C. , Ševeček P. , Gillon M. , Benkhaldoun Z. 
Published: August 2018

Context.The vast majority of the geophysical and geological constraints (e.g., internal structure, cratering history) for main-belt asteroids have so far been obtained via dedicated interplanetary missions (e.g., ESA Rosetta, NASA Dawn). The high angular resolution of SPHERE/ZIMPOL, the new-generation visible adaptive-optics camera at ESO VLT, implies that these science objectives can now be investigated from the ground for a large fraction ofD≥ 100 km main-belt asteroids. The sharp images acquired by this instrument can be used to accurately constrain the shape and thus volume of these bodies (hence density when combined with mass estimates) and to characterize the distribution and topography ofD≥ 30 km craters across their surfaces.Aims.Here, via several complementary approaches, we evaluated the recently proposed hypothesis that the S-type asteroid (89) Julia is the parent body of a small compact asteroid family that formed via a cratering collisional event.Methods.We observed (89) Julia with VLT/SPHERE/ZIMPOL throughout its rotation, derived its 3D shape, and performed a reconnaissance and characterization of the largest craters. We also performed numerical simulations to first confirm the existence of the Julia family and to determine its age and the size of the impact crater at its origin. Finally, we utilized the images/3D shape in an attempt to identify the origin location of the small collisional family.Results.On the one hand, our VLT/SPHERE observations reveal the presence of a large crater (D~ 75 km) in Julia’s southern hemisphere. On the other hand, our numerical simulations suggest that (89) Julia was impacted 30–120 Myrs ago by aD~ 8 km asteroid, thereby creating aD≥ 60 km impact crater at the surface of Julia. Given the small size of the impactor, the obliquity of Julia and the particular orientation of the family in the (a,i) space, the imaged impact crater is likely to be the origin of the family.Conclusions.New doors into ground-based asteroid exploration, namely, geophysics and geology, are being opened thanks to the unique capabilities of VLT/SPHERE. Also, the present work may represent the beginning of a new era of asteroid-family studies. In the fields of geophysics, geology, and asteroid family studies, the future will only get brighter with the forthcoming arrival of 30–40 m class telescopes like ELT, TMT, and GMT.
Fermi et l’Univers en rayons gamma
Authors: Ballet Jean , Grenier Isabelle 
Published: August 2018

En orbite autour de la Terre depuis juin 2008, l’observatoire gamma spatial Fermi balaye le ciel pour capter les rayons γ émis par les plus formidables accélérateurs de particules que la nature ait conçus, qu’il s’agisse d’étoiles à neutrons, de trous noirs, ou d’explosions stellaires.
Il nous révèle aussi le destin de ces particules de haute énergie qui sillonnent les galaxies et influencent leur évolution. Les observations de Fermi offrent de nouvelles perspectives sur ces phénomènes extrêmes qui défient souvent notre compréhension.
Pièges à fourmis
Authors: Crassous Jérôme , Humeau Antoine , Boury Samuel , Casas Jérôme 
Published: August 2018

Les animaux fabriquant des pièges pour capturer des proies sont relativement rares. L’exemple le plus connu est l’araignée qui utilise l’adhésion de sa toile pour fixer la proie. La larve de fourmilion fabrique dans du sable un piège qui tire parti des lois de la friction pour faire glisser la proie (généralement une fourmi) vers le prédateur.
C’est en observant certaines caractéristiques du piège de cet insecte que nous avons été amenés à étudier, puis à mettre en évidence, les particularités de la friction sur un matériau granulaire.
Le concours « Challenge Physique Expérimentale en Afrique »
Authors: Fokapu Odette , Hennequin Daniel , Lollman Dave , Piuzzi François , Suzor-Weiner Annick , Woafo Paul 
Published: August 2018

« Challenge Physique Expérimentale en Afrique » est un concours soutenu (entre autres) par la Société Française de Physique, et qui a pour objet le développement de dispositifs innovants à bas coût, réalisables localement autour du thème enseignement et recherche en physique expérimentale.
La première édition du concours s’est déroulée en 2017. Dix candidats sélectionnés ont reçu une formation expérimentale et ont présenté un projet comportant un module microcontrôleur. L’annonce du palmarès et la remise des prix ont eu lieu le 8 décembre 2017 au Cameroun, à Yaoundé.
Impact of Moderate Exercise on Post-concussive Symptoms and Cognitive Function after Concussion in Children and Adolescents Compared to Healthy Controls
Authors: Anderson Vicki , Manikas Vicky , Babl Franz , Hearps Stephen , Dooley Julian 
Published: August 2018

AbstractResolution of post-concussive symptoms (PCS) is a marker of recovery. However, it is unclear whether subtle impairments re-emerge in the context of increased demands. This study examined the effect of strenuous exercise on clinical symptoms and cognitive efficiency in children and adolescents after PCS resolution following concussion. We recruited concussed children and adolescents (n=30) on presentation to an Emergency Department (ED), and compared them to community-sourced non-injured controls (n=30), matched for age, and cognitive function. At Day 2 post symptom resolution, concussed participants (and controls) completed an exercise protocol, and pre- and post exercise assessments of PCS and computer-based cognitive efficiency. Findings demonstrated that, compared to controls, participants with recent concussion showed an overall reduction in PCS post-exercise (p=0.048), less impact of exercise on PCS than controls (p=0.021), and faster reaction times on computer-based cognitive tasks, even where cognitive demands were high (ps<0.05). These results provide preliminary support for the use of moderate exercise for the reduction of concussion post-symptom, as suggested in recent clinical guidelines.
Preoperative CT-Based Three-Dimensional Templating in Robot-Assisted Total Knee Arthroplasty More Accurately Predicts Implant Sizes than Two-Dimensional Templating
Authors: Pietrzak J. R. T. , Rowan F. E. , Kayani B. , Donaldson M. J. , Huq S. S. , Haddad F. S. 
Published: August 2018

AbstractPatient dissatisfaction after total knee arthroplasty (TKA) is a concern. Surgical error is a common, avoidable cause of failed TKA. Correct femoral and tibial component sizing improves implant longevity, clinical outcomes, knee balance, and pain scores. We hypothesized that preoperative three-dimensional (3D) templating for robot-assisted TKA (RA-TKA) is more accurate than two-dimensional (2D) digital templating. Prospectively collected data from 31 RA-TKAs were assessed to determine accuracy pertaining to implant sizing and positioning. All cases undergoing RA-TKA undergo preoperative CT-scans as per protocol. Three blinded observers retrospectively templated these knees for TKA using standard radiographs. We compared whether 2D templating was as accurate as CT-guided templating. Postoperative radiographs were then evaluated for sizing and positioning. Intraclass correlation coefficients (ICCs) and the effect of learning curve were assessed. Preoperative femoral component 3D templating and retrospective blinded 2D templating accuracies were 96.6% and 52.9%, respectively (χ
2: 17.965; odds ratio [OR]: 24.957, 3.250–191.661; p < 0.001). Tibial component 3D and 2D templating accuracies were 93.1% and 28.7%, respectively (χ
2: 36.436; OR: 33.480, 7.400–151.481; p < 0.001). ICC for the three radiograph observers was 0.920 (95% confidence interval [CI]: 0.652–0.890; p < 0.001) for the femur and 0.833 (0.717–0.911; p < 0.001) for the tibia, showing excellent agreement. We conclude that preoperative CT-based templating for RA-TKA more accurately predicts the size of implants compared with traditional 2D digital templating. This may improve operating room efficiency and cost containment.
Design and Implementation of a Pediatric ICU Acuity Scoring Tool as Clinical Decision Support
Authors: Shelov Eric , Muthu Naveen , Wolfe Heather , Traynor Danielle , Craig Nancy , Bonafide Christopher , Nadkarni Vinay , Davis Daniela , Dewan Maya 
Published: August 2018

Background and Objective Pediatric in-hospital cardiac arrest most commonly occurs in the pediatric intensive care unit (PICU) and is frequently preceded by early warning signs of clinical deterioration. In this study, we describe the implementation and evaluation of criteria to identify high-risk patients from a paper-based checklist into a clinical decision support (CDS) tool in the electronic health record (EHR).
Materials and Methods The validated paper-based tool was first adapted by PICU clinicians and clinical informaticians and then integrated into clinical workflow following best practices for CDS design. A vendor-based rule engine was utilized. Littenberg's assessment framework helped guide the overall evaluation. Preliminary testing took place in EHR development environments with more rigorous evaluation, testing, and feedback completed in the live production environment. To verify data quality of the CDS rule engine, a retrospective Structured Query Language (SQL) data query was also created. As a process metric, preparedness was measured in pre- and postimplementation surveys.
Results The system was deployed, evaluating approximately 340 unique patients monthly across 4 clinical teams. The verification against retrospective SQL of 15-minute intervals over a 30-day period revealed no missing triggered intervals and demonstrated 99.3% concordance of positive triggers. Preparedness showed improvements across multiple domains to our a priori goal of 90%.
Conclusion We describe the successful adaptation and implementation of a real-time CDS tool to identify PICU patients at risk of deterioration. Prospective multicenter evaluation of the tool's effectiveness on clinical outcomes is necessary before broader implementation can be recommended.
A framework to diagnose factors influencing proenvironmental behaviors in water-sensitive urban design
Authors: Schirmer Jacki , Dyer Fiona 
Published: August 2018

Significance
Urban stormwater runoff contributes significantly to water-quality problems in freshwater systems worldwide. Water-sensitive urban design (WSUD) typically addresses this through construction of ecologically sensitive infrastructure but rarely considers how encouraging change in human behaviors could contribute to improving water quality. The effectiveness of WSUD can be increased by encouraging adoption of proenvironmental behaviors that prevent water-quality deterioration at the source and improve the quality of water leaving stormwater systems, in turn reducing the investment needed in water-related infrastructure. The VAIL (values, awareness, identity, lifestyle) framework presented in this paper enables context-specific identification of factors that influence adoption of proenvironmental behaviors and can inform design of actions that seek to increase adoption of these behaviors.
Experimental measurement of the diamond nucleation landscape reveals classical and nonclassical features
Authors: Gebbie Matthew A. , Ishiwata Hitoshi , McQuade Patrick J. , Petrak Vaclav , Taylor Andrew , Freiwald Christopher , Dahl Jeremy E. , Carlson Robert M. K. , Fokin Andrey A. , Schreiner Peter R. , Shen Zhi-Xun , Nesladek Milos , Melosh Nicholas A. 
Published: August 2018

Significance
Nucleation is the limiting step for thermodynamic phase transitions. While classical models predict that nucleation should be extremely rare, nucleation is surprisingly rapid in the gas-phase synthesis of diamond, silicon, and other industrial materials. We developed an approach for measuring nucleation landscapes using atomically defined precursors and find that diamond critical nuclei contain no bulk atoms, which leads to a nucleation barrier that is four orders of magnitude lower than prior bulk estimations. Our findings suggest that metastable molecular precursors play a key role in lowering nucleation barriers during materials synthesis and provide quantitative support for recent theoretical proposals of multistep nucleation pathways with much lower barriers than the predictions of classical nucleation theory.
Invariances in the architecture of pride across small-scale societies
Authors: Sznycer Daniel , Xygalatas Dimitris , Alami Sarah , An Xiao-Fen , Ananyeva Kristina I. , Fukushima Shintaro , Hitokoto Hidefumi , Kharitonov Alexander N. , Koster Jeremy M. , Onyishi Charity N. , Onyishi Ike E. , Romero Pedro P. , Takemura Kosuke , Zhuang Jin-Ying , Cosmides Leda , Tooby John 
Published: August 2018

Significance
It has been proposed that one key function of pride is to guide behavior in ways that would increase others’ valuation of the individual. To incline choice, the pride system must compute for a potential action an anticipated pride intensity that tracks the magnitude of the approval or deference that the action would generate among local audiences. Data from industrial mass societies support this expectation. However, it is presently not known whether those data reflect cultural evolutionary processes or a panhuman adaptation. Experiments conducted in 10 traditional small-scale societies with widely varying cultures and subsistence modes replicate the pattern observed in mass societies. This suggests that pride is a universal system that is part of our species’ cooperative biology.