bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of high sensitivity c- reactive protein, Lipoprotein A and Troponin I levels in Predicting Cardiovascular Disease Severity on Coronary Angiogram- A Prospective Study
Authors: Khobragade Abhishek , Porwal Sanjay , Patted Suresh , Ambar Sameer , Prasad  , Metgudmath Vijayanand , Hesarur Vishwanath 
Published: November 2024

Abstract

Background:
Cardiovascular diseases (CVDs) continue to be the primary cause of death globally, significantly contributing to health deterioration and increased healthcare expenses. Annually, CVDs are responsible for more deaths than any other disease, causing approximately 17.9 million deaths, which equates to around 31% of global mortality. This study investigated the efficacy of high sensitivity c- reactive protein (hs-CRP), Lipoprotein A levels (Lipo A), and Troponin I (Trop I) levels in predicting CVD severity on coronary angiogram
Materials and Methods:
This prospective observational study included 211 patients aged 18 years and above, with a diagnosis of acute coronary syndrome (ACS), visiting the Department of Cardiology. Detailed history was recorded, and laboratory investigations included hs-CRP, Lipo A, Trop I, and Lipid Profile, and all the patients were subjected to coronary angiography. Coronary angiogram was studied and severity of CVD was noted, based on SYNTAX I and II.
Results:
The mean age of study patients was 62.82±10.17 years, with age ranging from 34 to 89 years, and majority being male (70.1%). 78.7% of patients were hypertensive and 71.6% were diabetic. Most common signs and symptom were chest pain (96.2%) and dyspnoea (87.2%). The higher levels of mean hs-CRP, Lipo A, Trop I, and LDL were significantly associated with severity of CVD on coronary angiogram (p<0.001).
Conclusion:
Higher levels of hs- CRP, Lipo A and Trop I can be a reliable predictor of severity of CVD on coronary angiograma.
An Outbreak Of Ralstonia Insidiosa In Hospitalized Children
Authors: Copana-Olmos Raul , Calderon-Lopez Maria , Ramos-Cardenas Roxana , Ledezma-Hurtado Wara , Calle-Cruz Hirma , Cano-Guzman Fatima 
Published: November 2024

Abstract

Introduction: Healthcare-associated infections are a public health problem, resulting in increased morbidity, mortality and costs to patients. The present study aims to analyze an in-hospital outbreak of Ralstonia insidiosa in children admitted to a pediatric referral hospital, with a focus on the control and prevention measures implemented. Methodology: An observational prospective study was conducted from the beginning of an outbreak of Ralstonia insidiosa to its end, with emphasis on the control measures implemented by a pediatric referral hospital. Results: We found 19 cases in 19 epidemiological weeks, with an attack rate of 1.76%, mainly affecting children hospitalized for more than 14 days, with invasive devices, admitted to pediatric intensive care (PICU) and with underlying disease. The clinical presentation was characterized by vomiting, fever and cough. The control of the outbreak was archived based on the installation of epidemiological and antimicrobial surveillance approach. Conclusions: We conclude that Ralstonia insidiosa is an emerging nosocomial, opportunistic and antibiotic-resistant pathogen affecting children with risk factors (invasive devices, immunocompromised and admitted to PICU), in whom it causes morbidity and mortality; furthermore, decision making based on a combined approach of epidemiological and antimicrobial surveillance with available resources can serve to control the outbreak.
The Effect of Using Topical Insulin on the Healing of Pressure Ulcers among Intensive Care Unit Patients
Authors: Qaysi Wafaa , Ghoul Ibrahim , Alshawish Eman 
Published: November 2024

Abstract

Background
Pressure ulcers pose significant challenges to healthcare systems, imposing a substantial burden on both patients and providers. Treatment strategies are diverse, but this study aimed to evaluate the effectiveness of topical insulin therapy in enhancing pressure ulcer healing among intensive care unit patients in Northern West Bank, Palestine.
Methods
An observational study in a Palestinian intensive care unit involved 68 patients split into two groups: one received topical insulin dressing, the other standard care. Patients were aged 18–70 with stage 2 or 3 pressure ulcers smaller than 10 cm². Exclusions included grade 1, 4, un-stage-able ulcers, insulin allergy, certain vascular diseases, pregnancy, immunodeficiency, and other conditions. The Braden Scale assessed ulcer risk on days 1, 3, 5, and 7, and the PUSH tool measured healing before and after treatment.
Results
The Insulin group had significantly better pressure ulcer healing than the Non-insulin group (p < 0.05). No significant differences were found in PUSH scores between diabetic and non-diabetic patients, gender, or blood sugar levels. Photographs confirmed these results.
Conclusion
Topical insulin is a safe, effective treatment for stage 2 and 3 pressure ulcers. Early skin assessment is recommended upon hospital admission, and further research is needed in larger populations.
Heterogeneity of IKZF1 genomic alterations and risk of relapse in childhood B-cell precursor acute lymphoblastic leukemia
Authors: Wangondu Ruth , Ashcraft Emily , Chang Ti-Cheng , Roberts Kathryn , Brady Samuel , Fan Yiping , Evans William , Relling Mary , Crews Kristine , Yang Jun , Yang Wenjian , Pounds Stanley , Wu Gang , Devidas Meenakshi , Maloney Kelly , Mattano Leonard , Schore Reuven , Angiolillo Anne , Larsen Eric , Salzer Wanda , Burke Michael , Loh Mignon , Jeha Sima , Pui Ching-Hon , Inaba Hiroto , Cheng Cheng , Mullighan Charles 
Published: November 2024

Abstract

Genomic alterations of IKZF1 are common and associated with adverse clinical features in B-ALL. The relationship between the type of IKZF1 alteration, disease subtype and outcome are incompletely understood. Leukemia subtype and genomic alterations were determined using transcriptome and genomic sequencing and SNP microarray in 688 pediatric patients with B-ALL in St. Jude Total Therapy 15 and 16 studies. IKZF1 alterations were identified in 115 (16.7%) patients, most commonly in BCR::ABL1 (78%) and CRLF2-rearranged, BCR::ABL1-like B-ALL (70%). These alterations were associated with 5-year cumulative incidence of relapse (CIR) of 14.8 ± 3.3% compared to 5.0 ± 0.9% for patients without any IKZF1 alteration (P < 0.0001). IKZF1 deletions of exon 4-7 (P = 0.0002), genomic IKZF1plus with any IKZF1 deletion (P = 0.006) or with focal IKZF1 deletion (P = 0.0007), and unfavorable genomic subtypes (P < 0.005) were independently adversely prognostic factors. Associations of genomic IKZF1plus and exon 4-7 deletions with adverse outcomes were confirmed in an independent cohort. Genomic IKZF1plus with any IKZF1 deletion, IKZF1 deletion of exon 4-7, and unfavorable subtype confer increased risk of relapse. The type of IKZF1 alteration, together with the subtype, are informative for risk stratification and predict response in patients with B-ALL.
Association between Echocardiographic Parameters of Cardiac Structure and Function and Mild Cognitive Impairment
Authors: Zhang Kai , Liu Xiaoxia , Huang Siyu , Liu Xinrui , Zhao Meiqi , Xue Chao , Xia Shijun , Dong Jianzeng , Kong Yu , Ma Changsheng 
Published: November 2024

Abstract

Background: Cardiovascular diseases (CVDs) marked with cardiac morphological or hemodynamical abnormalities are associated with mild cognitive impairment (MCI). The links between cardiac structure and function and MCI are not well understood. We aimed to explore the association between echocardiographic parameters of cardiac structure and function and MCI in CVD patients.
Methods: We conducted an age-, gender-, and education level-matched case-control study in general CVD participants with a 1:3 ratio of MCI (Montreal Cognitive Assessment [MoCA] score <26 and Mini-Mental State Examination [MMSE] score ≥24) and cognitively normal participants at a tertiary hospital in Beijing, China. The echocardiographic cardiac parameters and cognitive status were retrieved through the clinical electronic database from May 2021 to August 2023. Principal component analysis (PCA), negative binomial, and conditional multivariate regression were performed.
Results: A total of 1136 CVD participants (mean age, 61.1 ± 8.3 years) were included in the study, comprising 289 (25.3%) MCI and 847 cognitively normal participants. Compared to cognitively normal participants, MCI participants had a higher prevalence of left ventricular (LV) diastolic dysfunction (54.0% vs. 40.3%; P<0.001) and greater interventricular septal thickness (IVST) (1.04 ± 0.17 cm vs. 1.00 ± 0.20 cm; P=0.002). LV diastolic dysfunction (Beta [SE], 0.234 [0.045]; P<0.001) and IVST (Beta [SE], 0.034 [0.016]; P=0.036) were negatively correlated with the MoCA score of global cognitive function. LV diastolic dysfunction (OR, 2.03; 95% CI, 1.48-2.79; P<0.001) and IVST (OR, 1.14; 95% CI, 1.03-1.27; P=0.014) were positively associated with MCI, independent of diagnosed CVDs and the conventional MCI risk factors.
Conclusions: General CVD patients with abnormal echocardiographic LV diastolic dysfunction and IVST were associated with cognitive decline, suggesting further cognitive assessment for MCI.
Trial registration: Retrospectively registered.
A Tale of Two Mice: genetics of model mouse strains suggest a transcriptional basis for risk and resistance in idiopathic pulmonary fibrosis
Authors: Fennell Thea , Beržanskytė Ieva , Gam Rihab , Zhu Wencan , Sung Minkyung , Tandon Himani , Murray Lynne A. , Gough Julian 
Published: November 2024

Abstract

Background
Idiopathic pulmonary fibrosis (IPF) is a terminal inflammatory lung disease that causes permanent scarring (fibrogenesis). Bleomycin (BLM) is a drug used to induce fibrosis in mouse models, typically C57BL/6. However, meta-analyses show inter-strain heterogeneity in response, e.g. resistance in BALB/c. This study extends transcriptomic analysis of IPF to a resistant strain, qualifying inferences from the standard model and suggesting genetic risk factors to inform clinical research.
Methods
Transcriptomic datasets were generated from C57BL/6 and BALB/c mice. Test mice were administered BLM – with tissue samples sequenced from control, test, and contralateral lungs at the fibrogenesis stage of the BLM model (7–14 days after injection). Differentially expressed genes (DEGs) were calculated between treatments and strains, followed by gene network and transcription factor (TF) target enrichment analysis of DEGs. Additionally, strain-specific genetic variants were identified in fibrosis-related genes, complemented by analysing human genome-wide association (GWAS) datasets. An in vitro model of TGF𝛽-stimulated stress fibre deposition was used in parallel to confirm transcriptomic findings.
Results
DEGs calculated between treatment groups were enriched for general fibrosis-related processes across strains. Some fibrogenic processes and functional modules, however, were specifically enriched in C57BL/6, which was orthogonally validated by in vitro TGFβ assays. Conversely, the anti-fibrotic DEG Ctsk was upregulated under fibrosis in BALB/c specifically. TF target enrichment analysis of cross-strain and cross-treatment DEGs, using perturbation data, further identified them as significantly overrepresentative of FOSL1-sensitive genes. Subsequent genetic analysis revealed a non-conservative variant (P170L) located in BALB/c FOSL1. Furthermore, analysis of data from the 100,000 Genomes Project associated human FOSL1 variants with IPF.
Conclusions
Transcriptional differences in IPF have been characterised for C57BL/6 and BALB/c strains, supporting the consensus on IPF resistance in BALB/c. Analysis of gene set expression within and between strains principally implicates genes sensitive to the TF FOSL1. The significance of this novel finding is amplified by the discovery of a highly non-conservative P170L mutation in the bZIP domain of BALB/c FOSL1. Mechanistic investigation of FOSL1 activity – and potentially other regulators, e.g. Nos2, Il6 – is thus recommended as preclinical IPF research.
Characteristics, outcomes, and prognostic factors in patients with penetrating and blunt traumatic diaphragmatic injury: a nationwide retrospective cohort study in Japan
Authors: Shinjo Takafumi , Izawa Yoshimitsu , Yonekawa Chikara , Matsumura Tomohiro , Mato Takashi 
Published: November 2024

Abstract

Background
Traumatic diaphragmatic injury (TDI) is well-known worldwide as rare and life-threatening. However, because no nationwide cohort study of penetrating and blunt TDI has been conducted in Japan and other countries where penetrating trauma is relatively uncommon, the clinical characteristics of all TDI are unknown. Our purposes were to describe the characteristics of TDI patients, to compare penetrating TDI with blunt TDI, and to identify risk factors for mortality in Japan.
Methods
We retrospectively identified TDI patients between 2004 and 2019 using data from the Japan Trauma Data Bank. We extracted data on patient demographics, type of trauma, cause of trauma, physiological parameters, region of concomitant injury, associated injury, and management. We compared penetrating TDI and blunt TDI for each variable. The primary outcome was mortality. Multivariable logistic regression analysis was performed to identify risk factors for mortality.
Results
A total of 1147 patients (0.3%) had TDI, of which 771 were eligible for analysis (excluding 308 in cardiac arrest on arrival). Blunt TDI represented 70% and penetrating TDI 30%. In penetrating TDI, the most common cause was self-inflicted (49%), and compared to blunt TDI, males were 69% of patients (vs. 66%; P = 0.50) and the mortality rate was 8.3% (vs. 26%; P < 0.001). Multivariable analysis found that age (odds ratio [OR] 1.03, 95% confidence interval [CI] 1.01–1.04), Injury Severity Score (OR 1.03, 95%CI 1.006–1.06), Revised Trauma Score (OR 0.55, 95%CI 0.45–0.67), severe concomitant abdominal injury (OR 2.45, 95%CI 1.32–4.56), severe concomitant upper extremity injury (OR 3.38, 95%CI 1.24–9.17), computed tomography (CT) (OR 0.32, 95%CI 0.15–0.69), and diaphragm repair (OR 0.44, 95%CI 0.25–0.78) were independent predictors of mortality.
Conclusions
In Japan, we found that penetrating TDI was mainly caused by self-injury and the male-female ratio was the same as for blunt TDI, although blunt TDI was much more frequent. TDI was considered highly lethal, with over 25% of patients in cardiac arrest on arrival. Our unique independent predictors were CT, severe concomitant abdominal injury, and severe concomitant upper extremity injury. These findings may help in the management of TDI in countries with less common penetrating trauma.
Efficacy and Prognostic Factors of Surgical Resection for Pulmonary Metastases from Ovarian Cancer
Authors: Tsukamoto Yo , Ohtsuka Takashi , Yamauchi Yoshikane , Mun Mingyon , Matsuguma Haruhisa , Hashimoto Hiroshi , Fukuda Hiroki , Yosihno Ichiro , Shintani Yasushi , Kawamura Masafumi 
Published: November 2024

Abstract

Objective:
Pulmonary metastases from ovarian cancer are rare, and the efficacy of surgical intervention is unclear. This study aimed to validate the efficacy of surgical intervention for pulmonary metastases from ovarian cancer.
Methods:
Cases were taken from the database of the Metastatic Lung Tumor Study Group of Japan from 1996 to 2021, which prospectively registers surgical cases of pulmonary metastases at participating centers. Only patients who underwent radical surgery for pulmonary metastases from ovarian cancer were included. Factors associated with overall survival (OS) were analyzed.
Results:
The analysis included 48 patients with a mean age of 53.2 years old. The 5-year overall survival rate was 69.9% (95% confidence interval [CI] 51.9–82.2%), with a median survival period of 121 months (95% CI 64–134 months). Predictors of poorer OS included preoperative extrapulmonary metastasis (Hazard ratio [HR] 5.354, 95% CI 1.248–22.97; p = 0.024), elevated preoperative tumor marker levels (HR 2.999, 95% CI 1.028–8.705; p = 0.044), and a disease-free interval of less than 24 months (HR 4.355, 95% CI 1.004–18.89; p = 0.049). On multivariable analysis, preoperative extrapulmonary metastasis remained an independent prognostic factor (HR 6.229, 95% CI 1.216–31.92; p = 0.028).
Conclusions:
This study represents the largest number of patients for assessing the resection of pulmonary metastases from ovarian cancer. Preoperative extrapulmonary metastasis serves as an adverse prognostic factor, emphasizing the need for careful consideration of surgical indications.
The impact of a low-calorie, reduced-fat diet on liver attenuation imaging: A randomized clinical trial
Authors: Li Renjie , Li Jie , He Danni , Sui Yajuan , Liu Wenfen , Li Wentao , Meng Wenyi , Peng Jiahui , Xu Zuofeng 
Published: November 2024

Abstract

Purpose: To investigate whether a low-calorie, reduced-fat diet affects liver Attenuation Imaging (ATI) measurements.
Methods: A total of 320 patients were enrolled in this prospective study. They were randomly assigned to four groups: a fasting group; a postprandial 0.5-hour examination group; a postprandial 2-hour examination group; and a postprandial 4-hour examination group. All participants first underwent liver ATI examination in a fasting state. Those in the postprandial groups then consumed a low-calorie, reduced-fat diet before undergoing a second ATI examination at 0.5h, 2h, or 4h after the meal, respectively. The ATI values were compared among the groups. The differences between postprandial and fasting ATI values were also analyzed for the postprandial groups. Additionally, the consistency of the grading diagnosis of hepatic steatosis between the postprandial and fasting states was evaluated in the postprandial groups.
Results: The ATI values for the postprandial 0.5h group, postprandial 2h group, and postprandial 4h group were not significantly different from the fasting group's ATI value (P = 0.576, 0.471, and 0.992, respectively). No significant differences were noted in the ATI values that were recorded during the postprandial and fasting states within each of the postprandial groups (P= 0.573, 0.076, and 0.805, respectively). The consistency of the grading diagnosis of hepatic steatosis between the postprandial and fasting states across the three postprandial groups was high according to three different diagnostic criteria.
Conclusion: Consuming a low-calorie, reduced-fat diet has no significant effects on liver ATI measurements and the grading diagnosis of hepatic steatosis.
Clinical Trial Number: (ChiCTR2200062314,August 2022)
A methodology for building a medical ontology a with a limited domain experts’ involvement
Authors: Azzi Sabrina 
Published: November 2024

Abstract

Ontology development is a multidisciplinary work involving domain experts and knowledge engineers. Bringing together such a team to develop an ontology of quality is not easy. Therefore, ontologies are often created with limited expertise either in the medical domain or in ontology engineering. Unfortunately, the existing methodologies do not provide much guidance on how the different steps of ontology development should be performed, particularly in the case of reduced involvement of domain experts. This challenge is getting more difficult when there is a multitude of medical knowledge sources and ontologies covering parts of the domain, and often each having a different representation of the same concept. This research presents a methodology for creating a medical ontology of quality with limited involvement of the domain experts. The latter are only consulted in the domain definition and evaluation phases. We combine building an ontology from codified knowledge and ontology reuse to enhance reusability and interoperability. The methodology is inspired by METHONTOLOGY for which we make several improvements, especially in the ontology reuse phase. We provide a proof of concept of the proposed methodology with a case study involving the development of the pneumonia diagnosis ontology (PNADO).
Endoscopic Sleeve Gastroplasty as a therapeutic option for class III obesity
Authors: Bove Vincenzo , Pontecorvi Valerio , Gualtieri Loredana , Matteo Maria Valeria , De Siena Martina , Carlino Giorgio , Giannetti Giulia , Iaconelli Amerigo , Ficuccilli Ludovica , Ciasca Gabriele , Barbaro Federico , Spada Cristiano , Boskoski Ivo 
Published: November 2024

Abstract

Introduction
The spread of Endoscopic Sleeve Gastroplasty (ESG) as an effective bariatric endoscopic technique has been notable, particularly for treating patients with class I and II obesity with related comorbidities. Our study aimed to assess the ESG efficacy in patients with class III obesity.
Methods
This retrospective analysis on a prospective dataset focused on individuals with baseline class III obesity who underwent ESG from January 2018 to November 2023. Weight loss outcomes and the ESG impact on related comorbidities were assessed at 1, 3, 6, 12, and 24 months.
Results
A total of 129 patients [74 female, mean age: 47 ± 12.7, mean Body Mass Index (BMI): 44.4 ± 4.1 kg/m²], who either declined surgery or were considered ineligible for surgical intervention, were included. At the 24-months follow-up we observed a mean BMI reduction of 7.1 ± 4.8 and a mean Total Body Weight Loss of 15.4%. At the 12-months follow-up, our data showed improvement or complete remission of Type 2 Diabetes, Hyperinsulinemia, Arterial Hypertension, Obstructive Sleep Apnea Syndrome in 50%, 42.4%, 41.6% and 26% of patients with comorbidities, respectively. No serious adverse events were documented.
Conclusion
While ESG demonstrated effectiveness in inducing weight loss and ameliorating comorbidities, it did not fully resolve obesity, potentially due to high baseline BMI. Consequently, surgical intervention should continue to be the primary treatment for class III obesity. However, when surgery is contraindicated or refused by the patient, ESG represents a less invasive option and an effective first-stage or part of a multi-step treatment.
Hsp70 incompletely disaggregates the misfolding K488X-menin, promoting the tumorigenesis in a multiple endocrine neoplasia type 1 family
Authors: Zeng Zhen , Zhang Qianqian , Liang Tingting , Xiong Yu , Liu Zhi , Zhang Jing , Yang Pingping , Yang Jingye , Lu Qingxiang , Shen Di , Fang Wen , Zhang Miao , Liu Qi , Gao Bo , Wei Yonghui , Zhou Ding’an 
Published: November 2024

Abstract

Previous standpoints involved into the nonsense mutations and missense variants which caused truncated inactive menin protein of Multiple Endocrine Neoplasia Type 1(MEN1) gene, including loss of heterozygosity(LOH) and menin mutants degradation, cannot wholly interpret MEN1 pathogenesis. A c.1462A > T (p.K488X) mutation in exon10 of MEN1 was identified as the potential pathogenic mutation in an extended Chinese MEN1 family in this study. Ubiquitination modification degradation of K488X-menin result from the combined actions of carboxy-terminus of Hsc70-interacting protein (CHIP) and Hsp70 in vitro. K488X‒menin is a misfolding truncated protein that results in amyloid aggregation state in live cells or the affected tissues, the aggregation of which is promoted by Hsp70 and CHIP. Although Hsp70 disaggregate the aggregated of K488X-menin in vitro, the Hsp70 which is not upregulated in the MEN1 patients’ affected tissue can not completely disaggregate the aggregated K488X, which becomes a toxic intermediate to trigger development process of early tumorigenesis verified in the mutant zebrafish model and the affected tissues to cause the tumorigenic phenotypes in this MEN1 case. Our findings provide a complete novel interpretation to the mechanism of MEN1 tumorigenesis.