bluuebunny/crossref_metadata_2025_split huggingface dataset:
Natural products for denture base disinfection: A scoping review
Authors: de Lima Bianca Lopes de Andrade Martins , Santiago Jeferson Batista , Avelino Maria Eduarda Lemos , Vila‐Nova Taciana Emília Leite , Costa Rayanna Thayse Florêncio , Moraes Sandra Lúcia Dantas 
Published: November 2024

ObjectiveThis study aims to map the existing literature on natural products used as disinfection substances for conventional polymethyl methacrylate (PMMA) and computer‐aided design and manufacturing (CAD‐CAM) dentures.BackgroundDenture wearers are at high risk for denture stomatitis. Natural products have attracted the interest of the scientific community as an alternative to synthetic ones.Materials and methodsThe guiding question “Which natural products have been applied to disinfect complete dentures in conventional PMMA or CAD‐CAM PMMA resin?” Searches were conducted in the PubMed/MEDLINE, Embase, Web of Science, and Cochrane databases, and manual searches were performed in reference journals in the area with studies published until December 2023, without language or date restrictions.ResultsA total of 4272 articles were identified, and 46 studies were included after applying the eligibility criteria. Thirty‐nine natural products were evaluated. Of the included studies, 43 presented positive findings and three obtained negative findings on the effectiveness of natural products in inhibiting or killing Candida albicans.ConclusionNatural products, predominantly sourced from the Plantae kingdom, have demonstrated efficacy in reducing Candida albicans on the surface of conventional PMMA. However, the evidence primarily stems from in vitro studies, underscoring the necessity for additional clinical research to validate their applicability under real microbiological conditions in prosthesis users.
Dentists' perceptions of providing care to community‐dwelling older people
Authors: Friedlander Lara T. , Hamadani Payman , Chandler Nicholas P. , Daniel Ben K. 
Published: November 2024

ObjectivesThis study explored the attitudes of New Zealand (NZ) general dental practitioners (GDPs) towards older adults living within the community and the barriers and difficulties caring for their oral health needs.BackgroundAdults are living longer and retaining teeth. Their dentitions are complex and alongside their dental needs, older adults often experience age‐related systemic disease or decline. Ageism and stereotypical views of older adults impedes access to dental care. While there is some understanding of older adults and ageism from the perspective of dental students, there is a paucity of knowledge about dentists' management of this patient demographic.Materials and methodsA mixed‐methods survey questionnaire collected quantitative and qualitative data. Quantitative data were analysed descriptively followed by bivariate analysis. Qualitative data responses to open‐ended questions were analysed thematically.ResultsA total of 382 GDPs participated (response rate of 24%) and demonstrated positive attitudes and low levels of ageism towards older adults who they enjoyed treating. Confidence was positively related to clinical experience, and most dentists had engaged in gerodontology updates. Barriers to care for older adults were perceived to be beyond their control and mostly related to cost, chronic disease or age‐related change. Consent processes could create difficulty and so collaboration with family or caregivers was important.ConclusionThe GDPs displayed positive attitudes and confidence towards older adults who are a rewarding patient cohort. Gerodontology education during training and following graduation is critical to enhance oral health outcomes for older patients.
Effects of multiple‐dose administration of zavegepant nasal spray on the single‐dose pharmacokinetics of ethinyl estradiol‐levonorgestrel
Authors: Bhardwaj Rajinder , Collins Julie , Madonia Jennifer , Matschke Kyle , Bertz Richard , Liu Jing 
Published: November 2024

AbstractObjectiveThe potential for drug–drug interaction of multiple‐dose intranasal zavegepant on the single‐dose oral contraceptive ethinyl estradiol and levonorgestrel (EE‐LNG) was evaluated.BackgroundZavegepant (as a nasal spray) is a calcitonin gene‐related peptide receptor antagonist approved in the United States for treatment of acute migraine in adults.MethodsThis single‐center, Phase 1, open‐label, fixed‐sequence study included healthy, nonsmoking females (18–45 years old). In treatment Period 1, a single oral dose of EE‐LNG 0.02–0.10 mg was administered on Day 1. In treatment Period 2, intranasal zavegepant (20 mg daily; 10 mg per nostril separated by 1 h) was administered on Days 1–5; 1 oral dose of EE‐LNG 0.02–0.10 mg was administered immediately after first 10 mg intranasal zavegepant dose on Day 2. Blood samples for EE‐LNG concentrations were collected on Day 1, treatment Period 1, and Day 2, treatment Period 2, and zavegepant concentrations on Day 2, treatment Period 2. Noncompartmental pharmacokinetic parameters included maximum observed concentration (Cmax), area under the concentration‐time curve (AUC) from Time 0 to last non‐zero concentration (AUC0‐t), and AUC from Time 0 to infinity (AUC0‐inf). The safety and pharmacokinetic sample sizes were 26 and 23, respectively.ResultsStatistical comparisons of pharmacokinetic exposure parameters after co‐administration of zavegepant and EE‐LNG versus EE‐LNG alone showed small, but statistically insignificant, changes in either EE or LNG exposure. EE comparison ratios (90% confidence intervals [CIs]) were 109.9% (105.3%, 114.8%) for AUC0‐inf and 110.2% (104.6%, 116.1%) for Cmax. LNG comparison ratios (90% CIs) were 107.0% (100.2%, 114.3%) for AUC0‐inf and 108.8% (99.9%, 118.4%) for Cmax. Frequently reported treatment‐emergent adverse events included dysgeusia (n = 25, 96%), throat irritation (n = 11, 42%), headache (n = 10, 39%), nasal discomfort (n = 7, 27%), pharyngeal paresthesia (n = 5, 19%), and nausea (n = 4, 15%).ConclusionCo‐administration of zavegepant nasal spray with a single dose of an oral contraceptive resulted in no clinically meaningful changes (<12% increase) in EE‐LNG exposure.
Diagnostic Implications of CD63 and CD64 Expression Levels and FcγRIIIA 158 V/F Gene Polymorphism in Primary Immune Thrombocytopenia Adult Patients
Authors: Elsaid Dina Samir , Elbedewy Tamer Abd Elhamid , Soliman Nema Ali , Shalaby Kamal Ali , Haroun Riham Abdel‐Hamid 
Published: November 2024

ABSTRACTObjectiveImmune thrombocytopenic purpura (ITP) is an acquired autoimmune disease characterized by reduced platelet counts due to immune system dysregulation caused by many factors, including genetics, autoimmune diseases, infections, and inflammations. Therefore, the current study aimed to evaluate immunological markers such as the expression level of lysosomal associated membrane protein 3 (LAMP‐3), also known as CD63, and the expression level of Fc‐gamma receptor I (FcγRI), also known as CD64 and also investigate the association of Fc‐gamma receptor IIIA (FcγRIIIA) 158 V/F polymorphism to the risk of ITP.MethodsA total of 180 subjects; 60 ITP patients, 60 patients with thrombocytopenia of other causes and 60 controls were enrolled into our study. The expression level of CD63 was done using reverse transcription quantitative PCR (RTqPCR), while CD64 expression level was done by flow cytometry. The polymorphism of FcγRIIIA 158 V/F gene was analyzed by polymerase chain reaction followed by restriction fragment length polymorphism (PCR‐RFLP) analysis. Finally, CD63 and CD64 protein–protein interactions were done by using the STRING online database.ResultsThe expression of CD63 was significantly elevated in ITP patients than thrombocytopenia patients and healthy control. Also there was high expression level of CD64 on granulocytes and monocytes from ITP patients than other groups. Receiver operating characteristic curve (ROC curve) analysis of CD63 showed an area under the curve (AUC) revealed of 1.00, sensitivity of 100% and specificity of 100%; while for CD64 on granulocytes, AUC of 0.998 as well as a sensitivity of 96.66% and specificity of 93.33%. Regarding FcγRIIIa 158 V/F polymorphism, all patients and healthy volunteers included in this study showed the wild FF genotype.ConclusionsThe expression of both CD63 and CD64 were significantly increased in ITP patients and could be good biomarkers to diagnose ITP. Additionally, there is no association between FcγRIIIa 158 V/F polymorphism and the risk of ITP disease.
Reliability in design science research
Authors: Storey Veda C. , Baskerville Richard L. , Kaul Mala 
Published: November 2024

AbstractReliability, which is concerned with whether something performs as it should, is well‐accepted as an important part of any scientific research, with recognised methods for assessing it in the natural sciences. Reliability is a prerequisite for validity, which assesses knowledge production. Design science research is unique in that it produces an artefact and makes contributions to a design knowledge base and is tasked with the duality of design and science. Therefore, reliability should be reconsidered to address the assumptions underlying this type of information systems research.This paper proposes a Design Science Reliability Framework. The framework identifies the part of design science research that should be evaluated (artefact, methods, measures and design knowledge). It also considers when reliability should be assessed as a project progresses by differentiating synchronic versus diachronic evaluation. The framework is used to derive a set of techniques for establishing reliability that can be applied during the evaluation process to both a project's knowledge production and its artefacts. Application of the framework should assist design science researchers when considering how to assess their results.
Normalcy Among Individuals Living With Long‐Term Mechanical Circulatory Support: A Reflexive Thematic Analysis
Authors: Dzou Tiffany , Pieters Huibrie C. 
Published: November 2024

ABSTRACTAimTo explore descriptions of normalcy among individuals who have lived with mechanical circulatory support for a long time.DesignReflexive thematic analysis was used for this qualitative research.MethodsA parent study, utilising constructivist grounded theory, was conducted to explore the experiences of advance care planning among mechanical circulatory support individuals. Participants spontaneously shared their experiences of normalcy, which was outside the scope of the primary study. Thus, a secondary analysis using reflexive thematic analysis was performed to explore experiences of normalcy among individuals living with mechanical circulatory support for long‐term use.ResultsTwelve transcripts were purposively sampled and analysed. Three major themes were derived from the data: acquiescence, adapting to the device and restructuring family roles.ConclusionNormalcy continued to evolve years after device implantation because individuals were not prepared to face ongoing psychosocial challenges. Clinicians and researchers must address the complex emotional and social needs related to changes in goal therapy and unanticipated transplant delays. This includes the development of support groups that are aligned with the various stages of the MCS trajectory.Implications for the Profession and/or Patient CareClinicians need to engage patients in conversations about disruptions to their perceived ‘normal’ lifestyle and how they plan to adapt to complex changes. Future support groups can be organised according to individuals' duration of implantation and goal therapy to reduce social withdrawal. Additionally, clinicians should assess bridge to transplant individuals' attitudes before connecting them with transplanted volunteers. Finally, clinicians can support resilience by recognising and discussing the ongoing work required to adapt to the complex changes throughout the mechanical circulatory support trajectory.ImpactPerceptions of normalcy among mechanical circulatory support individuals are subject to ongoing change. Findings will inform clinicians of the social, emotional and familial challenges that require ongoing support and resources for long‐term mechanical circulatory support individuals.Reporting MethodReporting adhered to the COREQ checklist.Patient ContributionParticipants contributed to this study by sharing their experiences of normalcy after living with mechanical circulatory support for 2 years or more.
‘Why Is the Chubby Guy Running?’: Trans Pregnancy, Fatness, and Cultural Intelligibility
Authors: White Francis Ray , Pearce Ruth , Riggs Damien W. , Pfeffer Carla A. , Hines Sally 
Published: November 2024

ABSTRACTSince the late 2000s trans pregnancy has received increasing public and academic attention, and stories of the ‘pregnant man’ have become a media staple. Existing research has critiqued such spectacularization and the supposed tension between maleness, masculinity, and pregnancy that underpins it. Extending that work, this article draws on interview data from an international study of trans reproductive practices and analyzes participants' experiences of being, and expecting themselves to be, perceived in public space not as spectacularly ‘pregnant men’, but as fat men. As a starting point we take the experience of one participant whose heavily pregnant participation in a five‐kilometer race prompted the question: ‘Why is the chubby guy running?’ Using Judith Butler's concept of the cultural intelligibility of gender, we ask why the question asked was not: ‘Why is the pregnant guy running?’ We further consider the degree to which pregnant trans people manage their unintelligibility within the matrix of pregnancy, fatness, and trans/gender and how this reveals the limits of gender intelligibility itself.
Zinc finger transcription factors BnaSTOP2s regulate sulfur metabolism and confer Sclerotinia sclerotiorum resistance in Brassica napus
Authors: Dai Lihong , Xie Zhaoqi , Ai Tianxu , Jiao Yushun , Lian Xiaoyi , Long Angchen , Zhang Jinyun , Yang Guangsheng , Hong Dengfeng 
Published: November 2024

ABSTRACTRapeseed (Brassica napus L.) exhibits high‐sulfur requirements to achieve optimal growth, development, and pathogen resistance. Despite the importance of sulfur, the mechanisms regulating its metabolism and disease resistance are not fully understood. In this study, we found that the zinc finger transcription factors BnaSTOP2s play a pivotal role in sulfur metabolism and Sclerotinia sclerotiorum resistance. Our findings indicate that BnaSTOP2s are involved in sulfur metabolism, as evidenced by extensive protein interaction screening. BnaSTOP2s knockout reduced the content of essential sulfur‐containing metabolites, including glucosinolate and glutathione, which is consistent with the significantly lowered transcriptional levels of BnaMYB28s and BnaGTR2s, key factors involved in glucosinolate synthesis and transportation, respectively. Comprehensive RNA‐seq analysis revealed the substantial effect of BnaSTOP2s on sulfur metabolism from roots to siliques, which serve as pivotal sources and sinks for sulfur metabolism, respectively. Furthermore, we found that leaf lesion size significantly decreased and increased in the BnaSTOP2‐OE and Bnastop2 mutants, respectively, compared with the wild‐type during S. sclerotiorum infection, suggesting a vital role of BnaSTOP2s in plant defense response. In conclusion, BnaSTOP2s act as global regulators of sulfur metabolism and confer resistance to S. sclerotiorum infection in B. napus. Thus, they have potential implications for improving crop resilience.
Cascading effects driven by population recovery of sika deer on habitat use of sympatric mammals under heavy snow conditions
Authors: Yamashita J. , Akamatsu M. , Enari H. S. , Sembongi Y. , Enari H. 
Published: November 2024

AbstractUnlike the top‐down cascading effects driven by apex predators, the bottom‐up effects on biological interactions originating from herbivory by large mammals, especially within mammalian assemblages, have received less attention. This study aimed to identify the multifaceted impacts driven by sika deer (Cervus nippon) during midwinters, when their herbivory impacts were expected to be strong due to deficient dietary resources. We focused on interference and exploitative competition caused by deer on the habitat use of four sympatric mammal species, namely two herbivores (Japanese serow, Capricornis crispus, and Japanese hare, Lepus brachyurus angustidens) and two mesocarnivores (red fox, Vulpes vulpes, and Japanese marten, Martes melampus melampus). To estimate the wintering behaviors of these mammals and deer herbivory pressure, we performed snow tracking and counted the fresh feeding marks of deer during midwinter in 2022 and 2023. We conducted surveys on 201‐km transects with different stages of deer population recovery (i.e., different deer population densities) in heavy snowfall regions of Japan. We then systematically quantified the deer‐driven effects on sympatric mammals using piecewise structural equation modeling (SEM). We recorded 1700 tracks of target mammals and 1327 trees with deer feeding marks. Based on these records, we constructed an SEM with acceptable performance (Fisher's C = 27.1, P = 0.30). The SEM suggested some possibilities that exploitative competition mediated by deer herbivory constrained the habitat use of mammals with dietary habits similar to that of deer, i.e., serow (effect size, −0.13) and hares (−0.14). In addition, through constricting the habitat use of hares, the deer‐driven cascading effects reduced the occurrence of their key predators, i.e., foxes (−0.12) and martens (−0.10). Thus, our observations provide novel evidence that food webs are regulated by herbivore‐driven bottom‐up cascading effects in cases where plant primary productivity is limited by heavy snowfall.
Longer‐term effects of intermittent fasting on body composition and cardiometabolic health in adults with overweight and obesity: A systematic review and meta‐analysis
Authors: Khalafi Mousa , Maleki Aref Habibi , Ehsanifar Mahsa , Symonds Michael E. , Rosenkranz Sara K. 
Published: November 2024

SummaryThe aim of the present study was to investigate the effects of long‐term intermittent fasting (IF) on body composition and cardiometabolic health in adults with overweight and obesity. PubMed, Web of Science, and Scopus were searched from inception to March 2024 to identify original randomized trials that investigated the effects of IF versus either a control diet (CON) and/or continuous caloric restriction (CR). Participants were adults with overweight and obesity and intervention durations were ≥ 6 months. Overall, a total of 24 studies involving 2032 participants were included in the meta‐analysis. Compared with CON, IF significantly reduced body weight [WMD: −2.84 kg], BMI [WMD: −1.41 kg.m2], fat mass [WMD: −3.06 kg], fat‐free mass [WMD: −0.81 kg], waist circumference [WMD: −3.85 cm], visceral fat [SMD: −0.37], fasting glucose [WMD: −0.14 mmol/l], triglycerides [WMD: −0.12 mmol/l], and diastolic blood pressure [WMD: −2.24 mmHg]. Conversely, IF significantly increased high‐density lipoproteins [WMD: 0.04 mmol/l] when compared with CON, but had no effects on insulin, hemoglobin A1c%, total cholesterol, low‐density lipoprotein, or systolic blood pressure. Compared with CR, IF significantly reduced fat mass [WMD: −0.70 kg], body fat percentage [WMD: −0.59%], and DBP [WMD: −0.91 mmHg], and increased HDL [WMD: 0.03 mmol/l], with no other significant effects. Subgroup analyses showed that the mode of IF and intervention duration were the primary moderators of IF effects on the markers. In adults with overweight or obesity, IF and CR are comparably effective for reducing body weight and adiposity, as well as for improving cardiometabolic health markers.
Histone Lysine Lactylation (Kla)‐induced BCAM Promotes OSCC Progression and Cis‐Platinum Resistance
Authors: Huang Guangzhao , Chen Su , He Jialu , Li Honglin , Ma Zhongkai , Lubamba Grace Paka , Wang Lei , Guo Zhiyong , Li Chunjie 
Published: November 2024

ABSTRACTObjectiveHistone lysine lactylation (Kla) plays a vital role in cancer progression. However, the prognostic value of histone Kla in oral squamous cell carcinoma (OSCC) remains unclear.Materials and MethodsOSCC RNA expression data were obtained from the TCGA and GEO databases. We explored the prognostic value of histone Kla in OSCC via constructing a Cox model and determined the role of Kla in OSCC drug susceptibility and tumor microenvironment (TME). In addition, the biological roles of candidate biomarkers were identified.ResultsA total of 1223 Kla‐specific genes were obtained, with 228 DEKlaGs in OSCC. GO and KEGG enrichment analyses suggested that DEKlaGs contributed to inflammatory and cancer progression. A Cox model in accordance with BCAM, CGNL1, DGKG, and OLR1 could predict OSCC patient prognosis accurately. Subsequently, Kla‐induced prognostic genes were identified to play a crucial role in OSCC drug therapy and TME. Moreover, BCAM was identified as a biomarker that promoted OSCC invasion, angiogenesis, and chemotherapy resistance.ConclusionKla was identified to be associated with OSCC prognosis, drug therapy, and TME. Histone Kla‐induced BCAM was identified to play a crucial role during OSCC progression, suggesting that Kla is promising to be a novel therapeutic target for OSCC.
Hormone Replacement Therapy Relieves Periodontitis by Inhibiting Alveolar Bone Loss and Inflammation
Authors: Man Ying , Qin Jianyong , Qiu Hongliang , Wang Yiying , Zhang Xinyue , Yan Shengjie 
Published: November 2024

ABSTRACTObjectiveHormone replacement therapy (HRT) is a commonly used strategy for treating menopausal symptoms, while its relation with periodontitis remains unclear. This study aimed to explore the potential effects of HRT on periodontitis, mainly in aspects of bone loss and inflammation.MethodsThe alveolar bone height (ABH), alveolar bone thickness (ABT), and bone mineral density (BMD) were measured in menopausal women with periodontitis who had received HRT or had not received HRT by cone beam computed tomography. Based on a rat model of periodontitis, the alveolar bone loss was evaluated by micro‐computed tomography and bone‐related biochemical markers. The expression/levels of inflammatory markers were measured to reflect periodontal inflammation.ResultsAlthough the differences were not all significant in each premolars/molars, the mesial/distal ABH and buccal/lingual ABT were lower, and the mesial/distal BMD was higher in patients in the HRT group than those in the control group. In a rat model of periodontitis, the alveolar bone loss was relieved by HRT. Additionally, HRT significantly weakened the elevation of inflammatory markers, including TNF‐α, IL‐1β, and IL‐6 in periodontitis rats.ConclusionsHRT contributes to the remission of periodontitis by inhibiting alveolar bone loss and inflammation.