A novel green approach for treatment of immature Schistosomiasis Mansoni infection in mice; Arabic gum (Acacia Senegal) antischistosomal properties
Authors: Selem Rabab , Rashed Samia , Younis Mohammad , Hussien Boshra , Mohamed Fatma , Edrees Awatif , EL-kholy Asmaa , Rashed Gehan , Kishik Shereen , Moharm Ahlam , Nageeb Marwa , Kardoush Manal 
Published: June 2018

AbstractSchistosomiasis is one of the most socioeconomically exhausting parasitic infection in tropical and subtropical areas. Praziquantel (PZQ), the only common schistosocidal drug in use, is not efficient enough for treatment of immature infection. Arabic gum (AG) is a complex polysaccharide acts as anti-oxidant which modulates the inflammatory and/or immunological processes. This study explores for the first time, the antischistosomal properties of AG in mice infected with the immature stage ofSchistosoma mansoni. Mice were divided into four groups: control group (infected non-treated), AG treated group, PZQ treated group, and AG+PZQ treated group. Oral administration of AG in a dose of 1gm/kg body weight, daily for 3 consecutive weeks post-infection (p.i.) resulted in a statistically significant lower worm burden in both AG group and AG+PZQ group compared to PZQ and control groups. AG+PZQ group always showed the best performance when compared with other groups regarding tissue egg load and oogram pattern. AG, both alone and in combination with PZQ, decreased the number, diameter; increased the cellularity and the number of degenerated Schistosoma eggs inside granulomas. Results obtained by this work elucidated a promising AG bioactivity againstS. mansoniimmature stages and provided a platform for subsequent experimental studies to illuminate the academia more about this novel and “green” antischistosomal agent.Author summarySchistosomiasis is a major public health threat in many parts of the world, it affects more than 240 million people in more than 70 countries and almost 800 million people are at risk of acquiring this disease. Serious consequences and disabilities might result from untreated schistosomiasis such as hepatosplenic fibrosis with portal hypertension, gastrointestinal hemorrhage and death.Schistosomiasis control is focused on periodic treatment with praziquantel (PZQ). However, PZQ has only moderate action against young developing stages of schistosomula. Recently, resistance has emerged to PZQ. Therefore, chemotherapy alone is unlikely to reduce infection levels of schistosomiasis. Several practical approaches have been suggested to augment treatment programs. Of course, the development of a complementary treatment would contribute enormously to the reduction of schistosomiasis. Recently, natural products have been popular and attracted most of the attention as it could offer new effective therapy against schistosomiasis. Arabic gum (AG) is an edible, dried sticky exudate fromAcacia Senegal, which is used in this study to assess the AG antischistosomal properties. Our study revealed that AG has an excellent statistically significant effect against immature murine schistosomiasis, both alone and in combination with PZQ. This approach may point to novel targets for treatment of schistosomiasis.