bluuebunny/crossref_metadata_2025_split huggingface dataset:
Control of capillary behavior through target-responsive hydrogel permeability alteration for sensitive visual quantitative detection
Authors: Li Yansheng , Ma Yanli , Jiao Xiangyu , Li Tingyu , Lv Zhehao , Yang Chaoyong James , Zhang Xueji , Wen Yongqiang 
Published: March 2019

AbstractDNA hydrogels have received considerable attention in analytical science, however, some limitations still exist in the applications of intelligent hydrogels. In this paper, we describe a way to prepare gel film in a capillary tube based on the thermal reversible principle of DNA hydrogel and the principle of capillary action. Because of the slight change in the internal structure of gel, its permeability can be increased by the addition of some specific targets. The capillary behavior is thus changed due to the different permeability of the hydrogel film. The duration time of the target solution flowing through the capillary tube with a specified length is used to quantify this change. With this proposed method, ultra-trace DNA hydrogel (0.01 μL) is sufficient to realize the sensitive detection of cocaine without the aid of other instruments, which has a low detection limit (1.17 nM) and good selectivity.
Apoptotic tumor cell-derived microRNA-375 uses CD36 to alter the tumor-associated macrophage phenotype
Authors: Frank Ann-Christin , Ebersberger Stefanie , Fink Annika F. , Lampe Sebastian , Weigert Andreas , Schmid Tobias , Ebersberger Ingo , Syed Shahzad Nawaz , Brüne Bernhard 
Published: March 2019

AbstractTumor-immune cell interactions shape the immune cell phenotype, with microRNAs (miRs) being crucial components of this crosstalk. How they are transferred and how they affect their target landscape, especially in tumor-associated macrophages (TAMs), is largely unknown. Here we report that breast cancer cells have a high constitutive expression of miR-375, which is released as a non-exosome entity during apoptosis. Deep sequencing of the miRome pointed to enhanced accumulation of miR-375 in TAMs, facilitated by the uptake of tumor-derived miR-375 via CD36. In macrophages, miR-375 directly targets TNS3 and PXN to enhance macrophage migration and infiltration into tumor spheroids and in tumors of a xenograft mouse model. In tumor cells, miR-375 regulates CCL2 expression to increase recruitment of macrophages. Our study provides evidence for miR transfer from tumor cells to TAMs and identifies miR-375 as a crucial regulator of phagocyte infiltration and the subsequent development of a tumor-promoting microenvironment.
Inter-annual variation in seasonal dengue epidemics driven by multiple interacting factors in Guangzhou, China
Authors: Oidtman Rachel J. , Lai Shengjie , Huang Zhoujie , Yang Juan , Siraj Amir S. , Reiner Robert C. , Tatem Andrew J. , Perkins T. Alex , Yu Hongjie 
Published: March 2019

AbstractVector-borne diseases display wide inter-annual variation in seasonal epidemic size due to their complex dependence on temporally variable environmental conditions and other factors. In 2014, Guangzhou, China experienced its worst dengue epidemic on record, with incidence exceeding the historical average by two orders of magnitude. To disentangle contributions from multiple factors to inter-annual variation in epidemic size, we fitted a semi-mechanistic model to time series data from 2005–2015 and performed a series of factorial simulation experiments in which seasonal epidemics were simulated under all combinations of year-specific patterns of four time-varying factors: imported cases, mosquito density, temperature, and residual variation in local conditions not explicitly represented in the model. Our results indicate that while epidemics in most years were limited by unfavorable conditions with respect to one or more factors, the epidemic in 2014 was made possible by the combination of favorable conditions for all factors considered in our analysis.
LMO7 deficiency reveals the significance of the cuticular plate for hearing function
Authors: Du Ting-Ting , Dewey James B. , Wagner Elizabeth L. , Cui Runjia , Heo Jinho , Park Jeong-Jin , Francis Shimon P. , Perez-Reyes Edward , Guillot Stacey J. , Sherman Nicholas E. , Xu Wenhao , Oghalai John S , Kachar Bechara , Shin Jung-Bum 
Published: March 2019

AbstractSensory hair cells, the mechanoreceptors of the auditory and vestibular systems, harbor two specialized elaborations of the apical surface, the hair bundle and the cuticular plate. In contrast to the extensively studied mechanosensory hair bundle, the cuticular plate is not as well understood. It is believed to provide a rigid foundation for stereocilia motion, but specifics about its function, especially the significance of its integrity for long-term maintenance of hair cell mechanotransduction, are not known. We discovered that a hair cell protein called LIM only protein 7 (LMO7) is specifically localized in the cuticular plate and the cell junction. Lmo7 KO mice suffer multiple cuticular plate deficiencies, including reduced filamentous actin density and abnormal stereociliar rootlets. In addition to the cuticular plate defects, older Lmo7 KO mice develop abnormalities in inner hair cell stereocilia. Together, these defects affect cochlear tuning and sensitivity and give rise to late-onset progressive hearing loss.
Effects of chloride ions in acid-catalyzed biomass dehydration reactions in polar aprotic solvents
Authors: Mellmer Max A. , Sanpitakseree Chotitath , Demir Benginur , Ma Kaiwen , Elliott William A. , Bai Peng , Johnson Robert L. , Walker Theodore W. , Shanks Brent H. , Rioux Robert M. , Neurock Matthew , Dumesic James A. 
Published: March 2019

AbstractThe use of polar aprotic solvents in acid-catalyzed biomass conversion reactions can lead to improved reaction rates and selectivities. We show that further increases in catalyst performance in polar aprotic solvents can be achieved through the addition of inorganic salts, specifically chlorides. Reaction kinetics studies of the Brønsted acid-catalyzed dehydration of fructose to hydroxymethylfurfural (HMF) show that the use of catalytic concentrations of chloride salts leads to a 10-fold increase in reactivity. Furthermore, increased HMF yields can be achieved using polar aprotic solvents mixed with chlorides. Ab initio molecular dynamics simulations (AIMD) show that highly localized negative charge on Cl− allows the chloride anion to more readily approach and stabilize the oxocarbenium ion that forms and the deprotonation transition state. High concentrations of polar aprotic solvents form local hydrophilic environments near the reactive hydroxyl group which stabilize both the proton and chloride anions and promote the dehydration of fructose.
Cryo-EM structure of the human ferritin–transferrin receptor 1 complex
Authors: Montemiglio Linda Celeste , Testi Claudia , Ceci Pierpaolo , Falvo Elisabetta , Pitea Martina , Savino Carmelinda , Arcovito Alessandro , Peruzzi Giovanna , Baiocco Paola , Mancia Filippo , Boffi Alberto , des Georges Amédée , Vallone Beatrice 
Published: March 2019

AbstractHuman transferrin receptor 1 (CD71) guarantees iron supply by endocytosis upon binding of iron-loaded transferrin and ferritin. Arenaviruses and the malaria parasite exploit CD71 for cell invasion and epitopes on CD71 for interaction with transferrin and pathogenic hosts were identified. Here, we provide the molecular basis of the CD71 ectodomain-human ferritin interaction by determining the 3.9 Å resolution single-particle cryo-electron microscopy structure of their complex and by validating our structural findings in a cellular context. The contact surfaces between the heavy-chain ferritin and CD71 largely overlap with arenaviruses and Plasmodium vivax binding regions in the apical part of the receptor ectodomain. Our data account for transferrin-independent binding of ferritin to CD71 and suggest that select pathogens may have adapted to enter cells by mimicking the ferritin access gate.
CC chemokines are differentially expressed in Breast Cancer and are associated with disparity in overall survival
Authors: Thomas Jeronay K. , Mir Hina , Kapur Neeraj , Bae Sejong , Singh Shailesh 
Published: March 2019

AbstractDespite recent advances, breast cancer (BrCa) still affects many women and the impact is disproportional in African Americans (AA) compared to European Americans (EA). Addressing socioeconomic and behavioral status has not been enough to reduce disparity, suggesting contribution of biological differences in BrCa disparity. Our laboratory was first to show involvement of CC chemokines in BrCa. In this study, using ONCOMINE, TCGA, bc-GenExMiner and KMplotter, we examined the association of CC chemokines in BrCa outcomes and disparity. We show over-expression of CCL5, -7, -11, -17, -20, -22 and -25 in BrCa tissues. High mRNA levels of CCL7, -8, -17, -20 and -25 predicted a decrease in overall survival (OS). CCL7 and CCL8 were associated with decreased relapse-free survival. Expression of CCL17 and CCL25 was associated with decreased OS in AA. In EA, CCL8 was associated with decreased OS. Expression of CCL5, -7, -8, -17, -20 and -25 was highest in TNBC. Expression of CCL11 and CCL22 was associated with HER2. CCL7, -8, -17, -20 and -25 were elevated in AAs. In conclusion, our analysis suggests significant association of CC-chemokines in BrCa progression, OS and disparate disease outcome in AA compared to EA patients.
Cumulative and different genetic effects contributed to yield heterosis using maternal and paternal backcross populations in Upland cotton
Authors: Ma Lingling , Wang Yumei , Ijaz Babar , Hua Jinping 
Published: March 2019

AbstractHeterosis has been utilized in commercial production, but the heterosis mechanism has remained vague. Hybrid cotton is suitable to dissect the heterosis mechanism. In order to explore the genetic basis of heterosis in Upland cotton, we generated paternal and maternal backcross (BC/P and BC/M) populations. Data for yield and yield-component traits were collected over 2 years in three replicated BC/P field trials and four replicated BC/M field trials. At single-locus level, 26 and 27 QTLs were identified in BC/P and BC/M populations, respectively. Six QTLs shared in both BC populations. A total of 27 heterotic loci were detected. Partial dominant and over-dominant QTLs mainly determined yield heterosis in the BC/P and BC/M populations. QTLs for different traits displayed varied genetic effects in two BC populations. Eleven heterotic loci overlapped with QTLs but no common heterotic locus was detected in both BC populations. We resolved the 333 kb (48 genes) and 516 kb (25 genes) physical intervals based on 16 QTL clusters and 35 common QTLs, respectively, in more than one environment or population. We also identified 189 epistatic QTLs and a number of QTL × environment interactions in two BC populations and the corresponding MPH datasets. The results indicated that cumulative effects contributed to yield heterosis in Upland cotton, including epistasis, QTL × environment interaction, additive, partial dominance and over-dominance.
Analgesia after major laparoscopic surgery in patients with chronic kidney disease: A retrospective cohort study
Authors: Choi Hey-ran , Oh Tak Kyu , Kim Jinhee , Jeon Young-Tae 
Published: March 2019

AbstractThe amount of reduction in opioid doses and its effect on postoperative pain outcomes in chronic kidney disease (CKD) patients in the perioperative setting remains unclear. This study aimed to investigate differences in postoperative pain outcomes after major laparoscopic surgery between patients with CKD and those with normal preoperative kidney function. Medical records of patients who underwent laparoscopic major abdominal surgery from January 2010 to December 2016 were retrospectively reviewed, and 6,612 patients were finally included. During postoperative day (POD) 0–3, patients with an estimated glomerular filtration rate (eGFR) < 30 mL min−1 1.73 m−2 had 3.5% lower morphine equivalent consumption than those with an eGFR ≥ 90 mL min−1 1.73 m−2 (P = 0.023), whereas patients with preoperative eGFR between 60–90 mL min−1 1.73 m−2 and 30–60 mL min−1 1.73 m−2 showed no significant differences in morphine equivalent consumption. Additionally, pain scores at rest during POD 0–3 were not significantly associated with preoperative kidney function. In conclusion, our results suggest that patients with mild to moderate CKD (stage 2–3) did not require reduction of opioid analgesics during POD 0–3, compared to patients with normal preoperative kidney function. Only patients with severe CKD (stage ≥ 4) might require a slight reduction of opioid analgesics.
Development of an efficient cytosolic isobutanol production pathway in Saccharomyces cerevisiae by optimizing copy numbers and expression of the pathway genes based on the toxic effect of α-acetolactate
Authors: Park Seong-Hee , Hahn Ji-Sook 
Published: March 2019

AbstractIsobutanol production in Saccharomyces cerevisiae is limited by subcellular compartmentalization of the pathway enzymes. In this study, we improved isobutanol production in S. cerevisiae by constructing an artificial cytosolic isobutanol biosynthetic pathway consisting of AlsS, α-acetolactate synthase from Bacillus subtilis, and two endogenous mitochondrial enzymes, ketol-acid reductoisomerase (Ilv5) and dihydroxy-acid dehydratase (Ilv3), targeted to the cytosol. B. subtilis AlsS was more active than Ilv2ΔN54, an endogenous α-acetolactate synthase targeted to the cytosol. However, overexpression of alsS led to a growth inhibition, which was alleviated by overexpressing ILV5ΔN48 and ILV3ΔN19, encoding the downstream enzymes targeted to the cytosol. Therefore, accumulation of the intermediate α-acetolactate might be toxic to the cells. Based on these findings, we improved isobutanol production by expressing alsS under the control of a copper-inducible CUP1 promoter, and by increasing translational efficiency of the ILV5ΔN48 and ILV3ΔN19 genes by adding Kozak sequence. Furthermore, strains with multi-copy integration of alsS into the delta-sequences were screened based on growth inhibition upon copper-dependent induction of alsS. Next, the ILV5ΔN48 and ILV3ΔN19 genes were integrated into the rDNA sites of the alsS-integrated strain, and the strains with multi-copy integration were screened based on the growth recovery. After optimizing the induction conditions of alsS, the final engineered strain JHY43D24 produced 263.2 mg/L isobutanol, exhibiting about 3.3-fold increase in production compared to a control strain constitutively expressing ILV2ΔN54, ILV5ΔN48, and ILV3ΔN19 on plasmids.
Development of an SNP-based high-density linkage map and QTL analysis for bruchid (Callosobruchus maculatus F.) resistance in black gram (Vigna mungo (L.) Hepper)
Authors: Somta Prakit , Chen Jingbin , Yundaeng Chutintorn , Yuan Xingxing , Yimram Tarika , Tomooka Norihiko , Chen Xin 
Published: March 2019

AbstractBlack gram (Vigna mungo var. mungo) is an important pulse crop in Asia. The cowpea weevil (Callosobruchus maculatus) is a stored-seed insect pest (seed weevil/bruchid) that causes serious postharvest losses in pulse crops, including black gram. In this study, we constructed a high-density linkage map for black gram and identified quantitative trait loci (QTLs) for C. maculatus resistance. A recombinant inbred line (RIL) population of 150 lines from a cross between BC48 [cultivated black gram (var. mungo); bruchid-susceptible] and TC2210 [wild black gram (var. silvestris); bruchid-resistant] were used to construct a linkage map of 3,675 SNP markers from specific-locus amplified fragment sequencing. The map comprised 11 linkage groups spanning 1,588.7 cM with an average distance between adjacent markers of 0.57 cM. Seeds of the RIL population grown in 2016 and 2017 were evaluated for C. maculatus resistance through two traits; the percentage of damaged seeds (PDS) and infestation severity progress (AUDPS). Inclusive composite interval mapping identified three QTLs each for PDS and AUDPS. Two QTLs, qVmunBr6.1 and qVmunBr6.2, mapped about 10 cM apart on linkage group 6 were common between PDS and AUDPS. Comparative genome analysis revealed that qVmunBr6.1 and qVmunBr6.2 are new loci for C. maculatus resistance in Vigna species and that genes encoding a lectin receptor kinase and chitinase are candidates for qVmunBr6.2. The high-density linkage map constructed and QTLs for bruchid resistance identified in this study will be useful for molecular breeding of black gram.
Charge Storage by Electrochemical Reaction of Water Bilayers Absorbed on MoS2 Monolayers
Authors: Zhou Ruihua , Wei Sufeng , Liu Yan , Gao Nan , Wang Guoyong , Lian Jianshe , Jiang Qing 
Published: March 2019

AbstractIt is well-known that in neutral and acidic aqueous electrolytes, MoS2 monolayers can store charges by adsorption of cations on to the electrode-electrolyte interface as its analog of graphene. Restricted by its low conductivity and the charge storage mechanism, the electrochemical performance of MoS2 monolayer supercapacitor electrode is not satisfactory. It is reported here that water bilayers absorbed on MoS2 monolayers can be involved in charge storage. One proton of each absorbed water molecule can intercalate/de-intercalate the water bilayers during charging/discharging in the alkaline aqueous electrolyte. For two water molecules are present for every Mo atom, the water bilayers can endow MoS2 monolayers an ultrahigh specific capacitance. In this paper, 1T phase MoS2 nanosheets with three monolayers were synthesized by hydrothermal reaction. It presents a specific capacitance of 1120 F g−1 at a current density of 0.5 A g−1 in KOH. As it is assembled with active carbon into a hybrid supercapacitor, the device has an energy density of 31.64 Wh kg−1 at a power density of 425 W kg−1, and gets a specific capacitance retention of 95.4% after 10,000 cycles at 2 A g−1.
Reconstruction of noisy images via stochastic resonance in nematic liquid crystals
Authors: Feng Xingpan , Liu Hongjun , Huang Nan , Wang Zhaolu , Zhang Yongbin 
Published: March 2019

AbstractWe employ nematic liquid crystals as the nonlinear medium to recover noisy images via stochastic resonance, in which nonlinear coupling allows signals to grow at the expense of noise. The process is theoretically analyzed and the cross-correlation is numerically calculated. It is found that the quality of output images is affected by the input noise intensity, the applied voltage and the correlation length of noise light. Noise-hidden images can be effectively recovered by optimizing these parameters. The results suggest that nematic liquid crystals can be used for reconstruction of noisy images via stochastic resonance based on modulation instability with molecule reorientation nonlinearity.
Large intravalley scattering due to pseudo-magnetic fields in crumpled graphene
Authors: Kun Péter , Kukucska Gergő , Dobrik Gergely , Koltai János , Kürti Jenő , Biró László Péter , Tapasztó Levente , Nemes-Incze Péter 
Published: March 2019

AbstractThe pseudo-magnetic field generated by mechanical strain in graphene can have dramatic consequences on the behavior of electrons and holes. Here we show that pseudo-magnetic field fluctuations present in crumpled graphene can induce significant intravalley scattering of charge carriers. We detect this by measuring the confocal Raman spectra of crumpled areas, where we observe an increase of the D′/D peak intensity ratio by up to a factor of 300. We reproduce our observations by numerical calculation of the double resonant Raman spectra and interpret the results as experimental evidence of the phase shift suffered by Dirac charge carriers in the presence of a pseudo-magnetic field. This lifts the restriction on complete intravalley backscattering of Dirac fermions.
Organotin compound DBDCT induces CYP3A suppression through NF-κB-mediated repression of PXR activity
Authors: Li Yunlan , Lin Niu , Ji Xiaoqing , Mai Jiaqi , Li Qingshan 
Published: March 2019

Abstract
Organotin anticancer agent di-n-butyl-di-(4-chlorobenzohydroxamato)tin(iv) (DBDCT) exerted an inhibitory effect on its major metabolic enzyme cytochrome CYP3A. But whether hepatic drug-metabolizing enzymes and their regulatory nuclear receptors including pregnane PXR and constitutive androstane CAR binding with retinoid receptor RXR as a heterodimer are involved in the DBDCT-mediated regulation of CYP3A remains unclear. This study was undertaken to determine the mechanisms responsible for the effects of DBDCT on CYP3A suppression, focusing on the PXR-mediated and NF-κB pathways. The results indicated DBDCT suppressed CYP3A expression by inhibiting CAR expression. But what's interesting is, both protein and mRNA of PXR increased with increasing DBDCT. A further exploration, dual luciferase reporter gene analysis, clarified that DBDCT induced CYP3A expression elevation via the PXR-mediated pathway and this induction was countered by activation of NF-κB, which played a pivotal role in suppression of CYP3A through disrupting the association of the PXR–RXRα complex with DNA sequences by EMSA. PXR-mediated CYP3A expression was similarly demonstrated by RNAi. As expected, expression of CYP3A and its mRNA levels were reduced by DBDCT only in NF-κB(+/+) but not in NF-κB(−/−) cells. The inductive effect of DBDCT on CYP3A4 mRNA was enhanced in PXR shRNA-transfected cells but weakened in the ip65 group, which showed both PXR up-regulated CYP3A expression and NF-κB p65 activation directly contributed to CYP3A inhibition. In conclusion, activated NF-κB by DBDCT interacts directly with the DNA-binding domain of PXR, and disrupts the binding between the PXR–RXR dimer, thereby affecting the regulatory process for CYP3A transcription and, therefore, leading to a decrease of the expression of the PXR-regulated CYP3A.
Biological relevance of cell-in-cell in cancers
Authors: Mackay Hannah L. , Muller Patricia A.J. 
Published: March 2019

Abstract
Cell-in-cell (CIC) is a term used to describe the presence of one, usually living, cell inside another cell that is typically considered non-phagocytic. Examples of this include tumour cells inside tumour cells (homotypic), mesenchymal stem cells inside tumour cells (heterotypic) or immune cells inside tumour cells (heterotypic). CIC formation can occur in cell lines and in tissues and it has been most frequently observed during inflammation and in cancers. Over the past 10 years, many researchers have studied CIC structures and a few different models have been proposed through which they can be formed, including entosis, cannibalism and emperipolesis among others. Recently, our laboratory discovered a role for mutant p53 in facilitating the formation of CIC and promoting genomic instability. These data and research by many others have uncovered a variety of molecules involved in CIC formation and have started to give us an idea of why they are formed and how they could contribute to oncogenic processes. In this perspective, we summarise current literature and speculate on the role of CIC in cancer biology.