bluuebunny/crossref_metadata_2025_split huggingface dataset:
The development and co-design of a digitally supported intervention for selecting implementation tools and actions for standards (SITAS)
Authors: Kelly Yvonne , O'Rourke Niamh , Hegarty Josephine , Gannon Judy , Flynn Rachel , Keyes Laura M. 
Published: November 2024

Abstract

Background
Health and social care standards are multi-faceted interventions that promote quality and safe care in health systems. There is a need to apply evidence-based, rigorous and transparent methods when selecting strategies to support the implementation of standards. Researchers have consistently highlighted a research-to-practice gap in this area. We aimed to develop an evidence- and theory-informed intervention to guide researchers in identifying appropriate implementation strategies and then selecting tailored support tools and actions for use when implementing a set of standards.
Methods
Our intervention is a digital tool entitled ‘Selecting Implementation Tools and Actions for Standards (SITAS).’ We used multiple methods to develop SITAS, guided by programme theory. We identified enablers and barriers to implementing standards from the literature. We used these enablers and barriers alongside implementation science theories, tools and methodologies to construct SITAS. We refined SITAS using co-design workshops.
Results
Twenty-three enablers and barriers were identified. The Consolidated Framework for Implementation Research (CFIR) was identified as the best fit framework using the Theory Comparison and Selection Tool. The CFIR-ERIC (Expert Recommendations for Implementing Change) matching tool was used to identify matching implementation strategies. Enablers and barriers were mapped to the Behaviour Change Wheel (BCW). The enablers and barriers, outputs from the CFIR-ERIC matching tool and the BCW were used to develop a prototype of the content and interactive logic within a digital tool. The prototype was refined following feedback from intended users (n=7) at three workshops.
Conclusion
We developed a practical intervention enhanced by user involvement. SITAS can facilitate bridging the research-to-practice gap. It guides researchers through the process of selecting and tailoring implementation strategies to specific contexts, using core concepts of implementation science. Applying the CFIR-ERIC matching tool and BCW provided an integrated approach that linked the implementation strategies selected, their function and desired outcome. SITAS will need to undergo formal piloting before spread and scale-up.
What water-use traits contribute most to the maintenance of plant and grain production of African × Asian hybrid NERICA upland rice in arid soils?
Authors: Nguyen Hien Thi Thanh , Saitoh Kuniyuki , Kobata Tohru , Yamanaka Hiroto , Hirai Yoshihiko 
Published: November 2024

Abstract

New Rice for Africa (NERICA) upland rice is expected to have high production potential in sub-Saharan rainfed water-limited regions of Africa. Our objectives were to determine which of water use efficiency coefficient (k) and transpiration per vapor water deficit (T/VWD) strongly contribute to the maintenance of biomass production (BP) and grain (GP) production in desiccated soils in NERICA upland rice. Five NERICA upland cultivars and three Oryza sativa cultivars were compared under soil-moisture-deficit conditions after flag-leaf emergence. When the plants experienced different field capacities for 14 days in the 4-L pots, BP was expressed as the linear function of T/VWD with intercepts; hence, k as the slope was approximately constant across the different soil-moistures. In12-L pots with irrigation stopped for 10 days, T/T0 (T0 represents irrigated soils) for all the cultivars similarly decreased with a decrease in the fraction of transpirable soil water (FTSW). Two NERICA upland and one drought-sensitive Oryza sativa cultivars grown in 100 cm depth pots were irrigated from tubes set at four different depths. Under deep irrigation conditions resulting in dry surface and wet deep soils, the FTSW weighted by the root distribution (FTSWA) in the NERICA cultivars was greater than that in the sensitive Oryza sativa cultivar, where a greater FTSWA maintained BP, GP and the harvest index (HI). It was concluded that the most dominant trait for maintaining BP and GP under desiccated soils in NERICA upland rice was to ensure of T/VWD and HI by maintaining relatively high FTSWA through deep roots.
The potential mechanism of pachymic acid in triple-negative breast cancer: a network pharmacology and vitro experiments
Authors: Liu Ming , Zheng Li , Zhang Yang , Tian Jinhui 
Published: November 2024

Abstract

Background
Triple-negative breast cancer (TNBC) is characterized by the absence of estrogen and progesterone receptors, and lack of human epidermal growth factor receptor 2 (HER2) expression. Traditional Chinese medicine (TCM) has demonstrated promising efficacy in treating TNBC.
Objective
To explore the mechanisms of pachymic acid (PA) on TNBC.
Methods
We gained Microarray data of TNBC from the Gene Expression Omnibus (GEO). The related targets of PA were predicted and screened using the following six databases: Swiss Target, HERB, ETCM, BATMAN, HIT, and PharmMapper. Verification of Effective Targets by TCGA. The STRING interaction network analysis tool was used to create Protein-Protein Interaction (PPI) networks. Enrichment analysis included Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). We also conducted a pan-cancer analysis, tumor immune microenvironment analysis, and molecular docking. Finally, we performed cell experimental, included cytotoxicity assay, apoptosis analysis, proliferation assay, and migration and invasion assays.
Results
we found that PA has potential for treating TNBC, with TOP2A likely being its target, and platinum drug resistance possibly serving as the KEGG pathway through which PA exerts its therapeutic effects. By targeting TOP2A, PA is involved in processes such as nuclear division, chromosome segregation, mitotic nuclear division, condensed chromosome formation, and protein C-terminus binding. We found that PA may exert its therapeutic effects through the tumor immune microenvironment, involving elements such as Dendritic cells activated, Eosinophils, Macrophages M0, Macrophages M1, and T cells CD4 memory activated. The therapeutic effects of PA may vary across different subtypes of TNBC. We found that three subtypes, TNBC-BL1, TNBC-Metaplastic, and TNBC-BL2, are likely to respond more favorably.
Conclusion
Our study provides compelling evidence that PA holds significant promise as a therapeutic agent for TNBC, primarily through its action on TOP2A and its influence on the TNBC.
The underlying mechanism of cardiac injury in exertional heat stroke rats based on the scRNA-seq analysis
Authors: Luo Zhenghan , Li Zhi , Tang Chengliang , Zhang Jinhai , Chen Leru , Fu Qianyun , Zhang Qi , Yan Han , Yang Zhan , Wang Chunhui , Zheng Feng 
Published: November 2024

Abstract

Background
Heat dissipation relies on an intact cardiovascular system to dilate cutaneous blood vessels and increase cardiac output. However, the heart becomes a vulnerable organ and is susceptible to cardiac arrhythmias, functional failure, and focal myocardial necrosis in a hyperthermic heat-damaged state. In particular, people with cardiovascular dysfunction are at a much higher risk of exertional heat stroke (EHS). This study aimed to investigate and validate the cell signaling pathways and key genes associated with EHS by analyzing single-cell RNA sequencing (scRNA-seq) data from cardiac apical tissue of EHS rats. The findings are intended to elucidate the mechanisms underlying cardiac injury and to provide a theoretical basis for the early identification of biomarkers for cardiac injury in EHS.
Results
After exertional heat radiation, the heart's functionality was compromised. Annotation analysis revealed that the cell type and quantity did not differ between the EHS and control (CTL) groups. Cellchat analysis showed that the signal of EHS cardiac apex cells was enhanced in chemokine signaling pathway. The cardiac apical cells of the EHS group had the highest number of enriched genes in the oxidative stress pathway, according to GO/KEGG analysis of endothelial cells with the biggest proportion of cells. A total of 310 genes with changes in expression between the two groups were evaluated based on the Seurat-FindAllMarkers tools for all cell types. Of these, 18 genes with substantial variability were chosen for further verification. By using RT-qPCR verification, the expression differences of 12 genes were confirmed to be consistent with the above bioinformation analysis. Finally, Additional immunohistochemistry tests verified that Hspa8 and Hspe1 were up-regulated once more, while Id1, Ndufa4, and Cd36 were down-regulated.
Conclusions
The gene expression levels of Id1, Ndufa4, Cd36 were significantly reduced, and Hspa8, Hspe1 were significantly increased. These screened hypervariable genes play different roles in heat stress-induced mitochondrial and myocardial mechanical damage, protein misfolding, and they may become potential biomarkers in the mechanism of cardiac injury or keep an important link in the functional pathway of action described above.
Association between Gamma-glutamyl transpeptidase-to-lymphocyte ratio with hepatic steatosis and liver fibrosis: a nationally representative cross-sectional study from NHANES 2017 to 2020
Authors: Xiao Junyang , Jiang Yi , Liu Jianyong , Shen Jiajia , Cai Qiucheng 
Published: November 2024

Abstract

Background: The prediction and tracking of hepatic steatosis progression is of critical importance, yet there is a notable absence of reliable biomarkers. This study was conducted to identify a novel biomarker to enable accurate forecasting of hepatic steatosis and liver fibrosis.
Method: The data for this study were derived from the 2017-2020 National Health and Nutrition Examination Survey (NHANES). The multivariate linear regression models were used to investigate the relationship between the Gamma-glutamyl transpeptidase -to-lymphocyte ratio (GLR), controlled attenuation parameters (CAP), and liver stiffness measurements (LSM). The fitted smooth curve and threshold effect analyses were used to address non-linearity. Subgroup analyses were performed based on gender, age, diabetes, hypertension, and smoking.
Result: In total, 6481 patients were enrolled in the analysis. In the multivariate linear regression analysis, GLR is positively correlated with hepatic steatosis [CAP, β=0.29, 95% confidence interval (CI) (0.13, 0.44), P=0.0003]. This positive association is stable among all subgroups. An inverse L-shaped relationship between GLR and CAP was observed, with a stronger correlation when GLR<8.276. The correlation between GLR and liver fibrosis remained significantly linear in the multivariate regression analysis. [LSM β=0.03, 95% CI (0.02, 0.05), P<0.0001]. This association was more potent among participants over 50 years old (P for interaction < 0.05).
Conclusion: Our investigation revealed an association between GLR, CAP, and LSM. This association suggests that GLR holds predictive potential for assessing hepatic steatosis and liver fibrosis.
Are flexible ureteroscopes (FURS) equivalent in deflection when an anatomical deformation is applied? A comparative in vitro study of 10 FURS
Authors: Zahr Rawad Abou , Buhas Bogdan , Beauval Jean Baptiste , Lesourd Marine , Almeras Christophe 
Published: November 2024

Abstract

Purpose
Flexible ureteroscopy (FURS) has become indispensable in endoscopic urological practice. Multiple ureteroscopes with different characteristics were developed, the objective of the study was to determine if FURS are equivalent when put in anatomical conditions.
Methods
A comparative in vitro study of 10 different FURS was performed. Each ureteroscope deflection was measured before and after the introduction of a laser fiber of 272 µm by Rocamed© and a 1.5 Fr Nitinol stone basket Stonewrapper© in both the manufacturer’s conditions and in reproduced anatomic constraints. The tests were performed on a resin platform to apply necessary constraints using a 12-14Fr Coloplast© access sheath. The different angles of deflection were measured using AutoCad 2022©. Multiple other characteristics were measured for comparison and were: handle weight, mobile length, circular diameter, height at 180° of deflection, tip-shaft distance in full deflection and maximum reach from deflection point.
Results
The FURS tested were not equivalent in terms of loss in deflection when tested with reproduced anatomical constraints. A significant difference was found between the FURS tested with a range of + 11°/-75°. Our limitations are that the reproduced anatomical constraints do not represent the heterogeneity and complexity of human anatomy. Our study is limited to ten FURS and results might not be generalizable to all FURS in the market.
Conclusion
There is a loss of deflection capacity when anatomical deformation is applied to FURS. This can hinder treatment and thus must be acknowledged and known to urologists before selecting the corresponding ureteroscope.
Coastal zone classification and shoreline property identification based on random forests with dual-band LiDAR data
Authors: Jiang Huaigang , Zhang Zhenchao , Yu Ying , Dai Chenguang , Yi Ning , Hou Li , Xing Kan 
Published: November 2024

Abstract

Effective utilization of the coastal zone cannot be achieved without the support of strong mapping technology. With the growing technological advancements, LiDAR technology has found widespread applications in coastal zone mapping. Dual-band LiDAR integrates green and NIR wavelength lasers, offering the capability of acquiring both coastal terrain geometry and dual-band intensity simultaneously, providing complementary data that are temporally and spatially consistent.This study presents a framework for fusing dual-band echo intensity features together with geometric features for coastal zone classification and property identification of the extracted shoreline key points. It can directly extract the shoreline key points with property labels, which is a prerequisite for generating shorelines. Thus, the method provides an effective practical reference for more accurate measurement of the shoreline. Specifically, 26-dimensional geometric features are extracted from the coastal zone point cloud; the point cloud is then classified into five categories using the Random Forests; afterwards, the shoreline key point are extracted based on multiple coarse-to-fine grid constraints; finally, the shoreline key point are classified into three types. Experiments on the dual-band LiDAR data verifies that additional usage of dual-band echo intensity features improves the accuracy of coastal point cloud classification and shoreline property identification.
Astrocytes regulate brain fatty acid uptake and food intake via cluster of differentiation (CD)36 in diet-induced obesity
Authors: Timper Katharina , Coban Mustafa , Odermatt Timothy , Schmid Jochen , Abiven Pierre , Gnann Alexandra , Pedersen Kent , Stage Christina , Othman Alaa , Zamboni Nicola , Drogen Audrey van , Goetze Sandra , Karrer-Cardel Bettina , Handschin Christoph , Matter Matthias , Febbraio Maria , Wüest Stephan , Konrad Daniel , Clausen Trine , Jørgensen Sebastian 
Published: November 2024

Abstract

Upon high-fat diet (HFD) feeding, excessive amounts of saturated long-chain fatty acids (LCFAs) flood the brain via unknown mechanisms and induce a state of “lipotoxicity”, promoting overfeeding and obesity.
Here we demonstrate that astrocytes actively control brain LCFA shuttling via cluster of differentiation (CD)36. We found that HFD feeding induces CD36 expression in glial fibrillary acidic protein (GFAP)-expressing hypothalamic astrocytes, which allows for increased brain LCFA uptake. Brain bioluminescence-based analysis of brain FA uptake in genetically modified mouse models and metabolomics revealed that postnatal brain-wide astrocytic CD36 depletion partially protects from increased central FA uptake in diet-induced obese mice. On a cellular level, CD36 depletion in cultured primary hypothalamic mouse astrocytes impaired mitochondrial respiration due to a reduction in endogenous FA oxidation capacity. This resulted in a metabolic switch towards increased glycolysis and induced the integrated stress response marker growth differentiation factor 15 (GDF15) in vitro and in vivo. Astrocyte-specific CD36 depletion reduced body weight gain upon HFD feeding via decreased food intake and changed food preference towards low-fat chow diet, all mediated via enhanced central GDF15 activity.
Collectively, our results unravel a novel role of astrocytes as gatekeepers of brain LCFA uptake and that targeting astrocyte CD36 attenuates diet-induced obesity via increased hindbrain GDF15 signaling.
Peculiarities in thermal transport of nanostructured silicon arrays with different morphology
Authors: Chepela Lesia , Lishchuk Pavlo , Nkenfack Isibert , Mandrolko Viktor , Chaynes Hadrien , Kuzmich Andrey , Liu Poting , Borovyi Mykola , Lacroix David , Sivakov Vladimir , Isaiev Mykola 
Published: November 2024

Abstract

This study explores the thermal conductivity of silicon nanowires arrays produced by metal-assisted chemical etching of silicon wafers with different dopants, doping levels and crystallography. The wide range of morphological structures observed in silicon nanowires strongly depends on the initial wafer characteristics, a factor that cannot be neglected. While previous studies have demonstrated the qualitative capabilities of photoacoustic and Raman spectroscopy in characterising nanostructured silicon, our work highlights the quantitative discrepancies that can arise when combining these techniques to investigate thermal properties. The differences in the results obtained using these methods can be attributed to the distinct nature of the information they provide: photoacoustic spectroscopy probes the effective thermal conductivity over larger areas, whereas Raman spectroscopy offers localized measurements. Furthermore, our Monte Carlo simulations provide insights into the morphological features of porous silicon that influence the interpretation of experimental data. This study underscores the importance of a comprehensive approach, combining both experimental and theoretical methods, to assess the thermal transport properties of nanostructured materials accurately.
Sampled Surface and Shape DataBase (SSSDb): A database of deterministically sampled surface and a spectrum of shape representations of biomolecular structures
Authors: Karampudi Naga Bhushana Rao 
Published: November 2024

Abstract

Computational tools and techniques have come a long way in analyzing biomolecular structures. However, there are still limitations in how far we can exhaustively utilize the structural information of molecules in applications like whole database docking studies, achieving microsecond simulations , performing pairwise comparisions of entire molecular repositories. Coarse Grained models biomolecular structures have potential applications in many such methodologies as they help reduce the computational loads. SSSDb is a resource of such reduced representations of biomole-cular structures and provides surface and a spectrum of deterministically sampled shapes at varied fineness. Phase I of SSSDb provides 9,88,124 (≈ 1 million) coarse grained structures of 31,885 CATH protein domains. To cater the different possible needs of users the database is also provisioned to be accessible through four different options: (1) CATH ID based queires, (2) precompiled data clusters, (3) entire database download as single zip file and (4) customizable and automated downloads using programming script. In near future, SSSDb will be expanded to include all the biomolecular structures of Protein, DNA, RNA and their complexes. The entire database is available freely and openly for both academic and non-academic purposes. The database can be accessed at: https:// www.sssdb.nbrkarampudi.org/
Validation of the Spanish-language version of The Postpartum Specific Anxiety Scale Research Short Form: PSAS-ES-RSF
Authors: Costas-Ramón Natalia , Vinagre-González Agustina María , Silveiro Sergio A. , Fallon Victoria , García Marta Evelia Aparicio 
Published: November 2024

Abstract

Background:
Postpartum anxiety is a prevalent emotional disorder affecting approximately 20% of women, often more common than postpartum depression. Despite its high prevalence, the Spanish healthcare system lacks screening programs specifically targeting this condition.
Methods
This study aimed to develop and validate a short version of the Postpartum Specific Anxiety Scale (PSAS-ES-RSF) to address time constraints in clinical practice and improve its usability as a screening tool. Using confirmatory factor analysis (CFA) on two independent Spanish samples (Sample 1: N = 699; Sample 2: N = 293), a 16-item version was extracted from the original 51-item PSAS-ES.
Results
The four-factor structure of the PSAS-ES-RSF—addressing maternal competence, infant safety, practical infant care, and psychosocial adjustment—demonstrated strong psychometric properties (CFI > .95, RMSEA < .08). Cultural context and risk factors, such as maternal self-efficacy and the return to work, influenced the selection of items, showing some variation from the English-language short form.
Conclusions
The PSAS-ES-RSF offers a reliable and efficient tool for exploring postpartum anxiety in Spanish-speaking populations. Further research is needed to explore its applicability in diverse cultural contexts and to continue refining postpartum anxiety screening strategies.
Application of directional customized corneal relaxing incision in EVO ICL implantation: high myopia with low astigmatism
Authors: Cheng Mingrui , Liu Qingyang , Li Mingwei , Tang Shumin , Li Boliang , Jiang Yinjie , Lei Yadi , Lin I-chun , Xu Guanghan , Mao Zhiwei , Chen Xun , Wang Xiaoying 
Published: November 2024

Abstract

Purpose
To evaluate the efficacy of EVO-ICL implantation combined with a corneal relaxing incision for the correction of moderate to high myopia with low astigmatism.
Design:
prospective non-randomized clinical trial
Methods
This study included 35 eyes of 35 patients with astigmatism corrected by corneal relaxing incision (CRI) on the steep meridian and 30 eyes of 30 patients who underwent conventional temporal incision as controls. Visual acuity, refractive parameters, keratometry, corneal astigmatism, and corneal aberration were measured preoperatively and 1 and 3 months postoperatively.
Results
The refractive astigmatism of the CRI group decreased significantly from 0.89 ± 0.22 preoperatively to 0.39 ± 0.25 D at 1 month and 0.35 ± 0.30 D at 3 months postoperatively, and from 0.46 ± 0.32 D to 0.45 ± 0.34 D and 0.44 ± 0.30 D in the control group, respectively (P < 0.001). The preoperative anterior corneal astigmatism of the CRI group was significantly higher than the control group (P < 0.001) and decreased significantly from 1.03 ± 0.32 D preoperatively to 0.74 ± 0.36 D at 1 month and 0.69 ± 0.39 D at 3 months postoperatively (P < 0.001). The preoperative posterior corneal astigmatism of the CRI group decreased significantly from 0.32 ± 0.11 D preoperatively to 0.28 ± 0.12 D at 1 month and 0.25 ± 0.10 D at 3 months postoperatively (P < 0.001). There were significant differences in the postoperative RMS of trefoil aberration and total HOAs of the whole cornea, anterior cornea, and posterior cornea between the two groups (P < 0.05).
Conclusion
EVO-ICL implantation combined with a corneal relaxing incision to correct moderate-to-high myopia with low astigmatism is safe and effective with good short-term stability.