bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resilience and Well-Being Over the Course of an National Collegiate Athletic Association Division I Gymnastics Season
Authors: Williams Sarah-Margaret , Baez Shelby E. , Derrico Demitria , Robinson Hunter , Coyne Killian , Settle Hailee , Jochimsen Kate N. 
Published: December 2024

Context: Awareness regarding the importance of mental health in athletes has been increasing in recent years. Mental health has been investigated in relation to injury recovery and performance; however, less evidence has examined changes in mental health throughout a competitive season. Objectives: This study aimed to: (1) evaluate the psychological factors of resilience and well-being over a National Collegiate Athletic Association Division I gymnastics season and examine changes over 4 time points: preseason, start of the competitive season, midseason, and postseason and (2) examine the relationships between these psychological measures at these 4 time points. We hypothesized that resilience and well-being would remain stable, and higher resilience would be related to a greater sense of well-being at all time points. Design: This study utilized a retrospective chart review. Setting: This study is conducted in a college setting. Patients or Other Participants: Study participants were 17 Division I female gymnasts. Methods: A retrospective chart review was conducted to evaluate the Brief Resilience Scale and 5-item World Health Organization Well-Being Index, which gymnasts had completed biweekly from preseason to postseason. Descriptive statistics were reported, a 1-way repeated measures analysis of variance was used to examine changes across 4 time points, and Pearson product–moment correlations were used to examine the relationships between Brief Resilience Scale and 5-item World Health Organization Well-Being Index scores. Results: Brief Resilience Scale and 5-item World Health Organization Well-Being Index scores did not change throughout the season (P = .62, .52). No relationship between the variables was identified at the start of preseason or midseason (P > .17). However, at the start of the competitive season and postseason, resilience was positively correlated with well-being (r = .65, P = .006; r = .82, P = .006). Conclusions: Higher resilience and well-being are related in Division I female gymnasts but do not fluctuate throughout the season. Sports medicine staff and coaches should be aware that there is room to improve resilience and well-being, making additional supports warranted, including coping skills and resilience training interventions.
Effects of Neuromuscular Electrical Stimulation Waveforms and Occlusion Pressures on Elicited Force and Microvascular Oxygenation
Authors: Cayot Trent E. , Bellew James W. , Zapata-Rodriguez Estefania , Rutherford Justin , Simpson Sofia , Somesan Sam , Edgerton Trevor , Labaw Dawson , Northam Joe , Bowling Caleb 
Published: December 2024

Context: Interest in the effects of concurrently using neuromuscular electrical stimulation (NMES) and blood flow restriction (BFR) to improve muscle strength has risen, but limited studies and inconsistent findings have led to more questions. The 2 current projects aimed to systematically investigate how NMES waveform shape and BFR occlusion pressure acutely influence electrically elicited force (EEF) and tissue oxygen saturation (StO2) of the knee extensors. Design: A single-session repeated-measures design was followed. Methods: EEF and StO2 were measured in 2 different groups of 15 participants during 3 sets of NMES contractions. Ten NMES contractions per set were performed with 5 minutes of passive interset recovery. In the first project, different NMES waveforms (RUS, Russian burst-modulated alternating current; VMS, biphasic pulsed current; and VMS-Burst, burst-modulated biphasic pulsed current) were administered for each set, while BFR was applied at 60% limb occlusion pressure (LOP). During the second projet, VMS was administered, while a different BFR occlusion pressure (0% LOP, 40% LOP, and 80% LOP) was used during each set. Two-way repeated-measures analysis of variance examined if repetition and/or NMES waveform (first project) or BFR occlusion pressure (second project) significantly affected (P < .05) EEF or StO2. Results: VMS (12% [7%] MVIF) and VMS-Burst (13% [10%] MVIF) led to higher EFF compared with RUS (6% [5%] MVIF) with 60% LOP; 80% LOP (20% [14%] MVIF) led to lower EEF compared with 0% LOP (29% [17%] MVIF) with VMS. No significant differences in StO2 were observed between NMES waveforms or BFR occlusion pressures. Conclusions: If a clinician wanted to concurrently use NMES and BFR, the acute findings of the current projects would suggest the use of VMS or VMS-Burst with lower BFR occlusion pressure (40% LOP). However, further investigation into how these parameters would influence muscle strength subsequent to a training/rehabilitation intervention should be performed.
It’s a MuSt Win: The Effects of Self-Talk to Enhance Passing Performance Under Pressure in Elite Football Players
Authors: Viscosi Jonathan , Robazza Claudio , Jansson Billy , Davis Paul , Ruiz Montse C. 
Published: December 2024

Strategic self-talk is a powerful mental skill that athletes can use to self-regulate the effects of pressure and enhance task performance. Multistates (MuSt) theory considers the combination of emotion- and action-centered self-regulation to be the most effective strategy to optimize athletes’ performance and subjective experience. The aim of this study was to test the tenets of MuSt theory on a pressurized performance task, involving male junior football players (N = 20, Mage = 18.6) competing at the elite level in Finland. The task tested the players’ passing abilities over six trials while they applied different self-talk strategies in attempts to improve passing performance. The findings demonstrate the effectiveness of strategic self-talk as a form of self-regulation. Specifically, the combination of emotion- and action-centered cue words proved to be the optimal strategy for coping with the demands of the task. The study findings have relevant practical implications for players, coaches, and practitioners.
Complete genome sequences of
Pantoea vagans
strains GM1 and GM2, isolated from the leaves of garlic mustard
Authors: Shin Gi Yoon , Kvitko Brian H. 
Published: December 2024

ABSTRACT

We report the complete genomes of two
Pantoea vagans
strains, GM1 and GM2, isolated from garlic mustard plants.
P. vagans
strain GM1 was found to carry a HiVir pantaphos biosynthetic gene cluster, which causes onion necrosis.
Complete genome sequence of multidrug-resistant and NDM-producing
Klebsiella variicola
clinical isolates
Authors: Alvarado-Delgado Alejandro , Nava-Dominguez Neli , Rodriguez-Medina Nadia , Sánchez-Pérez Alejandro , Ortiz-Gutierrez Diana , Morfin-Otero Rayo , Rodriguez-Noriega Eduardo , Zumaya-Estrada Federico Alonso , Garza-Ramos Ulises 
Published: December 2024

ABSTRACT

Recently, global dissemination of NDM-producing
Klebsiella variicola
in hospital settings and natural environments has been described. This study described the whole-genome sequencing of multidrug-resistant phenotype and NDM-producing
Klebsiella variicola
clinical isolates.
Draft genome sequence of a halophilic, putative hydrocarbon-degrading
Arhodomonas
sp. strain KWT2
Authors: Ajagbe Damilare , Marsh William , Murdoch Robert , Fathepure Babu 
Published: December 2024

ABSTRACT

We report the draft genome sequence of a halophilic, putative hydrocarbon-degrading
Arhodomonas
sp. strain KWT2. The bacterium was isolated from a crude oil-contaminated saline pond in Kuwait. The genome is 3,923,868 bp in size and contains 3,534 predicted protein-coding genes, including genes for salt-tolerance and degradation of petroleum hydrocarbons.
Modelling the epidemiological and economic impact of digital adherence technologies with differentiated care for tuberculosis treatment in Ethiopia
Authors: Goscé Lara , Tadesse Amare Worku , Foster Nicola , van Kalmthout Kristian , Rest Job van , van der Wal Jense , Harker Martin J , Madden Norma , Abdurhman Tofik , Gadissa Demekech , Bedru Ahmed , Dube Tanyaradzwa N , Alacapa Jason , Mganga Andrew , Deyanova Natasha , Charalambous Salome , Letta Taye , Jerene Degu , White Richard , Fielding Katherine L , Houben Rein MGJ , McQuaid Christopher Finn 
Published: December 2024

BackgroundDigital adherence technologies (DATs) with associated differentiated care are potential tools to improve tuberculosis (TB) treatment outcomes and reduce associated costs for both patients and healthcare providers. However, the balance between epidemiological and economic benefits remains unclear. Here, we used data from the ASCENT trial to estimate the potential long-term epidemiological and economic impact of DAT interventions in Ethiopia.MethodsWe developed a compartmental transmission model for TB, calibrated to Ethiopia and parameterised with patient and provider costs. We compared the epidemiological and economic impact of two DAT interventions, a digital pillbox and medication labels, to the current standard of care, assuming each was introduced at scale in 2023. We projected long-term TB incidence, mortality and costs to 2035 and conducted a threshold analysis to identify the maximum possible epidemiological impact of a DAT intervention by assuming 100% treatment completion for patients on DAT.FindingsWe estimated small and uncertain epidemiological benefits of the pillbox intervention compared with the standard of care in Ethiopia, with a difference of −0.4% (95% uncertainty interval (UI) −1.1%; +2.0%) incident TB episodes and −0.7% (95% UI −2.2%; +3.6%) TB deaths. However, our analysis also found large total provider and patient cost savings (US$163 (95% UI US$118; US$211) and US$3 (95%UI: US$1; US$5), respectively, over 2023–2035), translating to a 50.2% (95% UI 35.9%; 65.2%) reduction in total cost of treatment. Results were similar for the medication label intervention. The maximum possible epidemiological impact a theoretical DAT intervention could achieve over the same timescale would be a 3% (95% UI 1.4%; 5.5%) reduction in incident TB and an 8.2% (95% UI 4.4%; 12.8%) reduction in TB deaths.InterpretationDAT interventions, while showing limited epidemiological impact, could substantially reduce TB treatment costs for both patients and the healthcare provider.
Second phase Chiba study of mother and child health (C-MACH): Japanese birth cohort study with multiomics analyses
Authors: Koshizaka Masaya , Eguchi Akifumi , Takaguchi Kohki , Yamamoto Midori , Takatani Rieko , Hisada Aya , Kawanami Akiko , Konno Yuki , Watanabe Masahiro , Tsumura Kayo , Shimatani Keiichi , Suzuki Norimichi , Mori Chisato , Sakurai Kenichi 
Published: December 2024

PurposeEpidemiological studies have reported that environmental factors from fetal period to early childhood can influence the risk of non-communicable diseases in adulthood. This concept has been termed the developmental origins of health and disease (DOHaD). The Chiba study of Mother and Child Health (C-MACH) is a DOHaD concept-based birth cohort study which started in 2014. This study aims to investigate the effects of genetic and environmental factors, particularly fetal and postnatal living environment, on children’s health. We also aim to identify candidate biomarkers for their health status. Moreover, the second phase study of C-MACH which was initiated in 2021 aimed at expanding the sample size, especially for gut microbiota and epigenomic analysis; it also aimed at clarifying the impact of the coronavirus disease 2019 (COVID-19) pandemic on children’s health.ParticipantsThis study consists of four hospital-based cohorts. Women who were <13 weeks pregnant and their partners were enrolled in the study. All data and biological samples will be stored in the Chiba University Centre for Preventive Medical Sciences.Findings to dateA total of 561 women and their partners provided their consent to participate in this study. Of these women, 505 completed the questionnaire during the early gestational period. The mean age of the 505 women at enrolment was 33.0 (SD, 4.5) years. The mean prepregnancy body mass index (BMI) was 21.7 (SD, 3.6) kg/m2, with 74.5% of the women having a BMI of 18.5–24.9 kg/m2. About 5.2% of the women smoked cigarettes during the early stages of pregnancy.Future plansThe primary study outcomes are allergies, obesity, endocrine and metabolic disorders and developmental difficulties in children. Variables related to genome, metabolome, epigenome, gut microbiota and exposome will be evaluated as health-related factors. The relationships between these outcomes and the health-related factors will be analysed.
Development of a parent decision support tool for surgical necrotising enterocolitis: a study protocol
Authors: Verhoeven Rosa , Kooi Elisabeth M W , Obermann-Borst Sylvia A , Geurtzen Rosa , Labrie Nanon H M , Verhagen A A Eduard , Hulscher Jan B F 
Published: December 2024

IntroductionNecrotising enterocolitis is a devastating gastrointestinal disease predominantly affecting preterm infants. In 40% of cases, its rapid progression renders conservative treatment insufficient, necessitating laparotomy as the sole viable option for survival. However, high perioperative and postoperative mortality rates, along with severe future potential disabilities and suffering, can complicate the decision of whether surgery is still in the infant’s best interest. In such cases, palliative care, aimed to minimise suffering, may be considered as an alternative to laparotomy, especially when the infant’s expected quality of life and overall prognosis are concerning. Depending on the sociocultural context, parents are increasingly involved in this decision. However, weighing the risks, benefits and uncertainties can be challenging for them. Therefore, we aim to develop a decision support tool using a novel combination of the Delphi technique and Q-methodology. Ultimately, we anticipate that this approach will contribute to improved family-centred care and optimised outcomes.Methods and analysisThe first phase of the study aims to identify key factors guiding Dutch parents’ decisions between laparotomy and palliative care (decision factors). Using a Delphi process, parents with varying perspectives and experiences will evaluate decision factors found in the literature and those self-suggested. The pertinent set of decision factors is defined during a consensus meeting.During the second phase, parents are asked to compare statements about these decision factors using Q-methodology. A by-person factor analysis of these comparisons will identify different parental decision-making profiles, which allows for formulating advice tailored to those profiles.Ultimately, we will build an online decision support tool which facilitates the classification of parent perspectives. The tool will then provide the parents with the relevant advice. In the last phase of the study, the tool’s effectiveness will be evaluated through an online questionnaire, asking parents to imagine using the tool in a real-world scenario.Ethics and disseminationEthical approval has been obtained from Central Ethics Review Committee of The University Medical Center Groningen (METc 2023/577, CTc UMCG 153660). Participants will be asked to provide their informed consent for the parts of the study that involve non-anonymous data gathering. Findings will be disseminated through academic journals and conferences. Options for long-term data preservation are under consideration.
Advancing a virtual home hospital: a blueprint for development and expansion
Authors: Mathura Pamela , Pascheto Isabella , Dytoc-Fong Haley , Hrynchyshyn Greg , McMurtry Natalie , Kassam Narmin 
Published: December 2024

BackgroundThe rising demand for hospitals has spurred increased interest in adopting virtual home hospital (VHH) care models. Development in this area often uses rigid research methods. This study describes a dynamic approach to constructing a VHH and outlines the progress over 5 years.MethodsIn 2018, a multicentre VHH was developed in Edmonton, Alberta, Canada, using an innovation lab approach, fostering collaboration among healthcare stakeholders for design, prototyping and testing. Over a 5-year period (2018–2022), the VHH underwent trial and adaptation using the Model for Improvement and the Dynamic Sustainability Framework, refining integrated care for a broader patient population. Within the VHH, patients received acute, hospital-level care at home, using technology, existing services and hospital and community personnel. Outcome measures included number of patient cohorts, staffing numbers, patients served, capacity and hospitals/health centres supported.ResultsOver 5 years, the VHH expanded from 2 to 15 staff members, from 14 to 25 physicians, from 45 to 870 total patients served, from 10- to 75-patient capacity and from serving 1 hospital to 6 hospitals and 1 health centre. The VHH advanced by transitioning from telehealth to digital remote patient monitoring, involving additional community partners, extending operating hours, diversifying admission and referral pathways and improving patient monitoring.ConclusionA VHH has the potential to bridge the gap between hospital and community care and to become a permanent healthcare delivery model that supports continuity of patient care.
Integration of lipidomics with targeted, single cell, and spatial transcriptomics defines an unresolved pro-inflammatory state in colon cancer
Authors: Soundararajan Ramani , Maurin Michelle M , Rodriguez-Silva Jetsen , Upadhyay Gunjan , Alden Ashley J , Gowda Siddabasave Gowda B , Schell Michael J , Yang Mingli , Levine Noah Jhad , Gowda Divyavani , Sundaraswamy Punith M , Hui Shu-Ping , Pflieger Lance , Wang Heiman , Marcet Jorge , Martinez Carolina , Bennett Robert David , Chudzinski Allen , Karachristos Andreas , Nywening Timothy M , Cavallaro Paul M , Anderson Matthew Linley , Coffey Robert J , Nebozhyn Michael V , Loboda Andrey , Coppola Domenico , Pledger Warren Jackson , Halade Ganesh , Yeatman Timothy J 
Published: December 2024

BackgroundOver a century ago, Virchow proposed that cancer represents a chronically inflamed, poorly healing wound. Normal wound healing is represented by a transitory phase of inflammation, followed by a pro-resolution phase, with prostaglandin (PGE2/PGD2)-induced ‘lipid class switching’ producing inflammation-quenching lipoxins (LXA4, LXB4).ObjectiveWe explored if lipid dysregulation in colorectal cancers (CRCs) is driven by a failure to resolve inflammation.DesignWe performed liquid chromatography and tandem mass spectrometry (LC–MS/MS) untargeted analysis of 40 human CRC and normal paired samples and targeted, quantitative analysis of 81 human CRC and normal paired samples. We integrated analysis of lipidomics, quantitative reverse transcription-PCR, large scale gene expression, and spatial transcriptomics with public scRNASEQ data to characterize pattern, expression and cellular localisation of genes that produce and modify lipid mediators.ResultsTargeted, quantitative LC–MS/MS demonstrated a marked imbalance of pro-inflammatory mediators, with a dearth of resolving lipid mediators. In tumours, we observed prominent over-expression of arachidonic acid derivatives, the genes encoding their synthetic enzymes and receptors, but poor expression of genes producing pro-resolving synthetic enzymes and resultant lipoxins (LXA4, LXB4) and associated receptors. These results indicate that CRC is the product of defective lipid class switching likely related to inadequate or ineffective levels of PGE2/PGD2.ConclusionWe show that the lipidomic profile of CRC tumours exhibits a distinct pro-inflammatory bias with a deficiency of endogenous resolving mediators secondary to defective lipid class switching. These observations pave the way for ‘resolution medicine’, a novel therapeutic approach for inducing or providing resolvins to mitigate the chronic inflammation driving cancer growth and progression.
DNA barcoded peptide-MHC multimers to measure and monitor minor histocompatibility antigen-specific T cells after allogeneic stem cell transplantation
Authors: Fuchs Kyra J , Göransson Marcus , Kester Michel G D , Ettienne Natasja W , van de Meent Marian , de Jong Rob C M , Koster Eva A S , Halkes Constantijn J M , Scheeren Ferenc , Heemskerk Mirjam H M , van Balen Peter , Falkenburg J H Frederik , Hadrup Sine R , Griffioen Marieke 
Published: December 2024

Allogeneic stem cell transplantation (alloSCT) provides a curative treatment option for hematological malignancies. After HLA-matched alloSCT, donor-derived T cells recognize minor histocompatibility antigens (MiHAs), which are polymorphic peptides presented by HLA on patient cells. MiHAs are absent on donor cells due to genetic differences between patient and donor. T cells targeting broadly expressed MiHAs induce graft-versus-leukemia (GvL) reactivity as well as graft-versus-host disease (GvHD), while T cells for MiHAs with restricted or preferential expression on hematopoietic or non-hematopoietic cells may skew responses toward GvL or GvHD, respectively. Besides tissue expression, overall strength of GvL and GvHD is also determined by T-cell frequencies against MiHAs.Here, we explored the use of DNA barcode-labeled peptide-MHC multimers to detect and monitor antigen-specific T cells for the recently expanded repertoire of HLA-I-restricted MiHAs. In 16 patients who experienced an immune response after donor lymphocyte infusion, variable T-cell frequencies up to 30.5% of CD8+T cells were measured for 49 MiHAs. High T-cell frequencies above 1% were measured in 12 patients for 19 MiHAs, with the majority directed against mismatched MiHAs, typically 6–8 weeks after donor lymphocyte infusion and at the onset of GvHD. The 12 patients included 9 of 10 patients with severe GvHD, 2 of 3 patients with limited GvHD and 1 of 3 patients without GvHD.In conclusion, we demonstrated that barcoded peptide-MHC multimers reliably detect and allow monitoring for MiHA-specific T cells during treatment to investigate the kinetics of immune responses and their impact on development of GvL and GvHD after HLA-matched alloSCT.