bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wildlife warning reflectors and white canvas reduce deer–vehicle collisions and risky road‐crossing behavior
Authors: Riginos Corinna , Graham Morgan W. , Davis Melanie J. , Johnson Andrew B. , May Alexander B. , Ryer Kevin W. , Hall L. Embere 
Published: March 2018

ABSTRACTCollisions between wildlife and vehicles are detrimental to both wildlife and human safety. A variety of mitigation methods have been deployed with the intent of increasing ungulate awareness of approaching vehicles. Wildlife warning reflectors are one such method. Roadside reflectors are designed to reflect headlights into the right‐of‐way and alert ungulates to approaching vehicles. Studies of the effectiveness of these reflectors have yielded mixed results. We conducted a robust test of reflectors in central Wyoming, USA, during 2013 and 2014, and unexpectedly discovered a potentially more effective method than the reflectors for reducing collisions between vehicles and mule deer (Odocoileus hemionus). In our initial experiment, we manipulated 10 1.6‐km segments of highway by leaving their reflectors exposed or covering them with a white canvas bag with the intention of neutralizing the reflector. The treatment configuration was swapped monthly. We counted deer carcasses under each treatment for 1 year and observed deer road‐crossing behavior using thermal video cameras. Carcass rates were 33% less in the white canvas treatment relative to uncovered reflectors. Deer in the white canvas treatment also stopped before entering the road 20% more often, ran into the road from the right‐of‐way 11% less often, and fled from the road 12% more often than when reflectors were uncovered. In a follow‐up experiment, we found that deer carcass rates were 32% less when reflectors were exposed versus covered with black canvas. We further found that deer road‐crossing behavior was least risky in a white canvas treatment, intermediate in a reflectors treatment, and most risky when reflectors were removed from posts. Taken together, these results indicate that, although reflectors were moderately effective, white canvas was substantially more effective in reducing deer–vehicle collisions. This unexpected finding suggests that new vigilance‐enhancing mitigation methods should be explored as a way to reduce wildlife–vehicle collisions. © 2018 The Wildlife Society.
An integrated workflow for phenazine-modifying enzyme characterization
Authors: Coates R Cameron , Bowen Benjamin P , Oberortner Ernst , Thomashow Linda , Hadjithomas Michalis , Zhao Zhiying , Ke Jing , Silva Leslie , Louie Katherine , Wang Gaoyan , Robinson David , Tarver Angela , Hamilton Matthew , Lubbe Andrea , Feltcher Meghan , Dangl Jeffery L , Pati Amrita , Weller David , Northen Trent R , Cheng Jan-Fang , Mouncey Nigel J , Deutsch Samuel , Yoshikuni Yasuo 
Published: March 2018

Abstract
Increasing availability of new genomes and putative biosynthetic gene clusters (BGCs) has extended the opportunity to access novel chemical diversity for agriculture, medicine, environmental and industrial purposes. However, functional characterization of BGCs through heterologous expression is limited because expression may require complex regulatory mechanisms, specific folding or activation. We developed an integrated workflow for BGC characterization that integrates pathway identification, modular design, DNA synthesis, assembly and characterization. This workflow was applied to characterize multiple phenazine-modifying enzymes. Phenazine pathways are useful for this workflow because all phenazines are derived from a core scaffold for modification by diverse modifying enzymes (PhzM, PhzS, PhzH, and PhzO) that produce characterized compounds. We expressed refactored synthetic modules of previously uncharacterized phenazine BGCs heterologously in Escherichia coli and were able to identify metabolic intermediates they produced, including a previously unidentified metabolite. These results demonstrate how this approach can accelerate functional characterization of BGCs.
Use of Tunneled-Cuffed Central Catheters in Patients with Cancer: A Single-Center Experience
Authors: Madabhavi Irappa , Patel Apurva , Anand Asha , Kataria Pritam , Kadakol Nagaveni , Sarkar Malay 
Published: March 2018

Abstract
Background: Effective and reliable venous access is among the cornerstones of modern medical therapy in oncology.
Materials and Methods: This was a prospective observational study of collected data of patients with a diagnosis of any cancer, at a tertiary care oncology hospital in Ahmadabad, Gujarat, India, during a 2-year period.
Results: A Hickman catheter was inserted in 200 patients and most commonly used in solid malignancies (n = 103; 51.5%), followed by hematologic conditions (n = 93; 48.5%). Among solid malignancies, hepatoblastoma (n = 21; 10.5%) was the most common indication, whereas in hematologic malignancies acute lymphoblastic leukemia was the most common indication (n = 56; 28%) for Hickman catheter insertion. Hickman catheters were inserted most commonly in the right side (n = 170; 85%) of the venous system. The various complications in the Hickman study group in descending order were 28 patients (14%) developed arrhythmias, 15 patients (7.5%) developed infection, 12 patients (6%) developed bleeding, 8 patients (4%) developed pneumothorax, 7 patients (3.5%) developed catheter blockage, and 6 patients (3%) required premature catheter removal. The median time of Hickman catheter in situ was 207 days.
Conclusions: The most disturbing aspect of treatment of patients with cancer is multiple painful venipunctures made for administration of cytotoxic agents, antibiotics, blood products, and nutritional supplements. The focus of this prospective observational research was to study the various indications for Hickman catheter in different solid and hematologic malignancies as well as the various complications and outcomes in pediatric and adult cancer patients.
Lean Six Sigma for Intravenous Therapy Optimization: A Hospital Use of Lean Thinking to Improve Occlusion Management
Authors: Steere Lee , Rousseau Marc , Durland Lisa 
Published: March 2018

Abstract
Background: Continual improvement is a necessary part of hospital culture. This occurs by identifying opportunities for improvement that influence efficiency while saving money.
Methodology: An investigation of intravenous device-related practices was performed by the nurses of the intravenous access team, pharmacy, and hospital operations at Hartford Hospital using Lean Six Sigma methodology. Central venous access device occlusion and tissue plasminogen activator variability was identified. Using observation, measurement of performance, and root cause analysis, the hospital's practices, policies, and equipment were evaluated for the process of occlusion management. The team utilized a Six Sigma strategy employing the elements define, measure, analyze, improve, and control, which is a disciplined, data-driven methodology that focuses on eliminating defects (waste). Interventions initiated based on the assessment performed by the team using the define, measure, analyze, improve, and control approach included replacement of negative displacement needleless connectors with antireflux needleless connectors and specialty team assessment before tissue plasminogen activator use.
Results: Over the course of the 26-month study, Hartford Hospital experienced a 69% total reduction in tissue plasminogen activator use representing a total 26-month savings of $107,315. Other cost savings were reflected in areas of flushing, flushing disposables, and in a decrease in needleless connector consumption. Central line-associated bloodstream rates fell 36% following the intervention as an unexpected secondary gain, resulting in further savings related to treating this nonreimbursable hospital-acquired condition.
Conclusions: This study examined the influence of using Lean Thinking and Six Sigma methodology as a tool in saving hospital money, resulting in better patient outcomes.
Alcohol use and personality trait change: pooled analysis of six cohort studies
Authors: Hakulinen Christian , Jokela Markus 
Published: March 2018

AbstractBackgroundPersonality has been associated with alcohol use, but less is known about how alcohol use may influence long-term personality trait change.MethodsThe present study examines associations between alcohol use and change in the five major personality traits across two measurement occasions (mean follow-up of 5.6 years). A total of 39 722 participants (54% women) were pooled from six cohort studies for an individual-participant meta-analysis. Alcohol use was measured as (1) average alcohol consumption, (2) frequency of binge drinking, (3) symptoms of alcohol use disorder, and (4) a global indicator of risky alcohol use. Changes in the five major personality traits (extraversion, emotional stability, agreeableness, conscientiousness, and openness to experience) were used as outcomes.ResultsRisky alcohol use was associated with increasing extraversion [0.25 T-scores over the mean follow-up of 5.6 years; 95% confidence interval (CI) 0.07–0.44] and decreasing emotional stability (−0.28; 95% CI −0.48 to −0.08), agreeableness (−0.67; 95% CI −0.87 to −0.36), and conscientiousness (−0.58; 95% CI −0.79 to −0.38). Except the association between alcohol use and extraversion, these associations were consistent across cohort studies and across different measures of alcohol use.ConclusionsThese findings suggest that alcohol use is associated with personality trait changes in adulthood.
Habitat selection by the black-tufted marmosetCallithrix penicillatain human-disturbed landscapes
Authors: Secco Helio , Grilo Clara , Bager Alex 
Published: March 2018

Abstract:There are several primate species with high risk of extinction in small forest fragments disturbed by human activities. However, some species exhibit high ecological plasticity, which allows them to persist in human-modified landscapes. The main goal of this study is to examine the relative roles of vegetation (mean distance among trees and mean canopy cover), human disturbance (distance to the road, distance to the fragment edge and edge type), and habitat spatial configuration (spatial autocorrelation index, category of quadrat – position within the fragment/fragment type) on the habitat selection of the black-tufted marmosetCallithrix penicillatain forest patches. We selected forest patches near and away from roads/urban areas, in southern Minas Gerais state, south-eastern Brazil. We used generalized linear mixed models to explain the presence of black-tufted marmoset in those patches. Our results show that black-tufted marmoset tend to occupy forest fragments closer to roads/urban areas, and consequently are under the influence of anthropic disturbance. In addition to the area delimited by these fragments, there is a preference for edge environments, where disturbances are exacerbated and the ecological conditions are suitable for exploitation by the black-tufted marmoset (supply of gum trees and reduced risk of competition). We suggest that a cross-habitat spillover by marmoset occur from forests to small habitat patches close to human-modified areas, such as those in proximity to roads and urban zones.
Acquisition of locative utterances in Norwegian: structure-building via lexical learning
Authors: MITROFANOVA Natalia , WESTERGAARD Marit 
Published: March 2018

AbstractThis paper focuses on the acquisition of locative prepositional phrases in L1 Norwegian. We report on two production experiments with children acquiring Norwegian as their first language and compare the results to similar experiments conducted with Russian children. The results of the experiments show that Norwegian children at age 2 regularly produce locative utterances lacking overt prepositions, with the rate of preposition omission decreasing significantly by age 3. Furthermore, our results suggest that phonologically strong and semantically unambiguous locative items appear earlier in Norwegian children's utterances than their phonologically weak and semantically ambiguous counterparts. This conclusion is confirmed by a corpus study. We argue that our results are best captured by the Underspecified P Hypothesis (UPH; Mitrofanova, 2017), which assumes that, at early stages of grammatical development, the underlying structure of locative utterances is underspecified, with more complex functional representations emerging gradually based on the input. This approach predicts that the rate of acquisition in the domain of locative PPs should be influenced by the lexical properties of individual language-specific grammatical elements (such as frequency, morphological complexity, phonological salience, or semantic ambiguity). Our data from child Norwegian show that this prediction is borne out. Specifically, the results of our study suggest that phonologically more salient and semantically unambiguous items are mastered earlier than their ambiguous and phonologically less salient counterparts, despite the higher frequency of the latter in the input (Clahsen et al., 1996).
Cooperation, competition and antibiotic resistance in bacterial colonies
Authors: Frost Isabel , Smith William P J , Mitri Sara , Millan Alvaro San , Davit Yohan , Osborne James M , Pitt-Francis Joe M , MacLean R Craig , Foster Kevin R 
Published: March 2018

Abstract
Bacteria commonly live in dense and genetically diverse communities associated with surfaces. In these communities, competition for resources and space is intense, and yet we understand little of how this affects the spread of antibiotic-resistant strains. Here, we study interactions between antibiotic-resistant and susceptible strains using in vitro competition experiments in the opportunistic pathogen Pseudomonas aeruginosa and in silico simulations. Selection for intracellular resistance to streptomycin is very strong in colonies, such that resistance is favoured at very low antibiotic doses. In contrast, selection for extracellular resistance to carbenicillin is weak in colonies, and high doses of antibiotic are required to select for resistance. Manipulating the density and spatial structure of colonies reveals that this difference is partly explained by the fact that the local degradation of carbenicillin by β-lactamase-secreting cells protects neighbouring sensitive cells from carbenicillin. In addition, we discover a second unexpected effect: the inducible elongation of cells in response to carbenicillin allows sensitive cells to better compete for the rapidly growing colony edge. These combined effects mean that antibiotic treatment can select against antibiotic-resistant strains, raising the possibility of treatment regimes that suppress sensitive strains while limiting the rise of antibiotic resistance. We argue that the detailed study of bacterial interactions will be fundamental to understanding and overcoming antibiotic resistance.
Orchestrated control of filaggrin–actin scaffolds underpins cornification
Authors: Gutowska-Owsiak Danuta , de La Serna Jorge Bernardino , Fritzsche Marco , Naeem Aishath , Podobas Ewa I. , Leeming Michael , Colin-York Huw , O’Shaughnessy Ryan , Eggeling Christian , Ogg Graham S. 
Published: March 2018

AbstractEpidermal stratification critically depends on keratinocyte differentiation and programmed death by cornification, leading to formation of a protective skin barrier. Cornification is dynamically controlled by the protein filaggrin, rapidly released from keratohyalin granules (KHGs). However, the mechanisms of cornification largely remain elusive, partly due to limitations of the observation techniques employed to study filaggrin organization in keratinocytes. Moreover, while the abundance of keratins within KHGs has been well described, it is not clear whether actin also contributes to their formation or fate. We employed advanced (super-resolution) microscopy to examine filaggrin organization and dynamics in skin and human keratinocytes during differentiation. We found that filaggrin organization depends on the cytoplasmic actin cytoskeleton, including the role for α- and β-actin scaffolds. Filaggrin-containing KHGs displayed high mobility and migrated toward the nucleus during differentiation. Pharmacological disruption targeting actin networks resulted in granule disintegration and accelerated cornification. We identified the role of AKT serine/threonine kinase 1 (AKT1), which controls binding preference and function of heat shock protein B1 (HspB1), facilitating the switch from actin stabilization to filaggrin processing. Our results suggest an extended model of cornification in which filaggrin utilizes actins to effectively control keratinocyte differentiation and death, promoting epidermal stratification and formation of a fully functional skin barrier.
JMJD5 is a human arginyl C-3 hydroxylase
Authors: Wilkins Sarah E. , Islam Md. Saiful , Gannon Joan M. , Markolovic Suzana , Hopkinson Richard J. , Ge Wei , Schofield Christopher J. , Chowdhury Rasheduzzaman 
Published: March 2018

AbstractOxygenase-catalysed post-translational modifications of basic protein residues, including lysyl hydroxylations and Nε-methyl lysyl demethylations, have important cellular roles. Jumonji-C (JmjC) domain-containing protein 5 (JMJD5), which genetic studies reveal is essential in animal development, is reported as a histone Nε-methyl lysine demethylase (KDM). Here we report how extensive screening with peptides based on JMJD5 interacting proteins led to the finding that JMJD5 catalyses stereoselective C-3 hydroxylation of arginine residues in sequences from human regulator of chromosome condensation domain-containing protein 1 (RCCD1) and ribosomal protein S6 (RPS6). High-resolution crystallographic analyses reveal overall fold, active site and substrate binding/product release features supporting the assignment of JMJD5 as an arginine hydroxylase rather than a KDM. The results will be useful in the development of selective oxygenase inhibitors for the treatment of cancer and genetic diseases.
A spliced latency-associated VZV transcript maps antisense to the viral transactivator gene 61
Authors: Depledge Daniel P. , Ouwendijk Werner J. D. , Sadaoka Tomohiko , Braspenning Shirley E. , Mori Yasuko , Cohrs Randall J. , Verjans Georges M. G. M. , Breuer Judith 
Published: March 2018

AbstractVaricella-zoster virus (VZV), an alphaherpesvirus, establishes lifelong latent infection in the neurons of >90% humans worldwide, reactivating in one-third to cause shingles, debilitating pain and stroke. How VZV maintains latency remains unclear. Here, using ultra-deep virus-enriched RNA sequencing of latently infected human trigeminal ganglia (TG), we demonstrate the consistent expression of a spliced VZV mRNA, antisense to VZV open reading frame 61 (ORF61). The spliced VZV latency-associated transcript (VLT) is expressed in human TG neurons and encodes a protein with late kinetics in productively infected cells in vitro and in shingles skin lesions. Whereas multiple alternatively spliced VLT isoforms (VLTly) are expressed during lytic infection, a single unique VLT isoform, which specifically suppresses ORF61 gene expression in co-transfected cells, predominates in latently VZV-infected human TG. The discovery of VLT links VZV with the other better characterized human and animal neurotropic alphaherpesviruses and provides insights into VZV latency.
Dietary butyrate glycerides modulate intestinal microbiota composition and serum metabolites in broilers
Authors: Yang Xiaojian , Yin Fugui , Yang Yuhui , Lepp Dion , Yu Hai , Ruan Zheng , Yang Chengbo , Yin Yulong , Hou Yongqing , Leeson Steve , Gong Joshua 
Published: March 2018

AbstractButyrate can modulate the immune response and energy expenditure of animals and enhance intestinal health. The present study investigated changes in the intestinal microbiota composition and serum metabolites of young broilers in response to 3,000 ppm butyrate in the form of butyrate glycerides (BG) via pyrosequencing of bacterial 16S rRNA genes and nuclear magnetic resonance (NMR). The dietary treatment did not affect the alpha diversity of intestinal microbiota, but altered its composition. Thirty-nine key operational taxonomic units (OTUs) in differentiating cecal microbiota community structures between BG treated and untreated chickens were also identified. Bifidobacterium was, in particular, affected by the dietary treatment significantly, showing an increase in not only the abundance (approximately 3 fold, P ≤ 0.05) but also the species diversity. The (NMR)-based analysis revealed an increase in serum concentrations of alanine, low-density and very low-density lipoproteins, and lipids (P ≤ 0.05) by BG. More interestingly, the dietary treatment also boosted (P ≤ 0.05) serum concentrations of bacterial metabolites, including choline, glycerophosphorylcholine, dimethylamine, trimethylamine, trimethylamine-N-oxide, lactate, and succinate. In conclusion, the data suggest the modulation of intestinal microbiota and serum metabolites by BG dietary treatment and potential contribution of intestinal bacteria to lipid metabolism/energy homeostasis in broilers.
Profiling walking dysfunction in multiple sclerosis: characterisation, classification and progression over time
Authors: Filli Linard , Sutter Tabea , Easthope Christopher S. , Killeen Tim , Meyer Christian , Reuter Katja , Lörincz Lilla , Bolliger Marc , Weller Michael , Curt Armin , Straumann Dominik , Linnebank Michael , Zörner Björn 
Published: March 2018

AbstractGait dysfunction is a common and relevant symptom in multiple sclerosis (MS). This study aimed to profile gait pathology in gait-impaired patients with MS using comprehensive 3D gait analysis and clinical walking tests. Thirty-seven patients with MS walked on the treadmill at their individual, sustainable speed while 20 healthy control subjects walked at all the different patient’s paces, allowing for comparisons independent of walking velocity. Kinematic analysis revealed pronounced restrictions in knee and ankle joint excursion, increased gait variability and asymmetry along with impaired dynamic stability in patients. The most discriminative single gait parameter, differentiating patients from controls with an accuracy of 83.3% (χ2 test; p = 0.0001), was reduced knee range of motion. Based on hierarchical cluster and principal component analysis, three principal pathological gait patterns were identified: a spastic-paretic, an ataxia-like, and an unstable gait. Follow-up assessments after 1 year indicated deterioration of walking function, particularly in patients with spastic-paretic gait patterns. Our findings suggest that impaired knee/ankle control is common in patients with MS. Personalised gait profiles and clustering algorithms may be promising tools for stratifying patients and to inform patient-tailored exercise programs. Responsive, objective outcome measures are important for monitoring disease progression and treatment effects in MS trials.