bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic Profiling and Quantitative Comparison of Chemical Components in Fagopyri Dibotryis Rhizoma From Different Habitats and Growing Periods Based on Ultra‐High‐Performance Liquid Chromatography With Mass Spectrometry
Authors: Wu Didi , Qu Cheng , Yu Daixin , Yang Fan , Wu Qinan 
Published: December 2024

ABSTRACTFagopyri Dibotryis Rhizoma, a traditional Chinese medicine, is widely used to treat various ailments such as pulmonary abscesses, measles pneumonia, and swelling. Its notable therapeutic effects are closely related to the chemical constituents, making it crucial to conduct in‐depth research on the chemical components. In this study, a total of 209 compounds were preliminarily identified using ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry in Fagopyri Dibotryis Rhizoma. Subsequently, a method was established using ultra‐high‐performance liquid chromatography‐triple quadrupole/linear ion trap tandem mass spectrometry for the simultaneous determination of 24 main ingredients (flavonoids and phenolic acids) in Fagopyri Dibotryis Rhizoma from different habitats and growing periods. Quantitative results showed that different compounds displayed varying contents across different habitats, with catechin, epicatechin, procyanidin B2, and procyanidin C1 being relatively abundant. Principal component analysis and partial least squares discriminant analysis showed there were significant differences among Fagopyri Dibotryis Rhizoma from different habitats, and the quality from Yunnan was superior. Entropy weight TOPSIS analysis showed that Fagopyri Dibotryis Rhizoma grown until late October has better comprehensive quality, which is basically consistent with the traditional harvesting time. In summary, this study can provide reference methods for the comprehensive evaluation of the quality of Fagopyri Dibotryis Rhizoma.
Development and Validation of a Sensitive LC–MS/MS Method for Determination of Lenvatinib and Its Major Metabolites in Human Plasma and Its Application in Hepatocellular Carcinoma Patients
Authors: Guan Shaoxing , Yuan Guosheng , Bi Guofang , Yu Qingqing , Fang Jian‐Hong , Chen Jinzhang , Bi Huichang 
Published: December 2024

ABSTRACTLenvatinib has been demonstrated effective in advanced hepatocellular carcinoma (HCC), but the pharmacokinetic–pharmacodynamics behavior of lenvatinib and its metabolites remains unclear. To investigate the pharmacokinetic–pharmacodynamics behavior of lenvatinib and its active metabolites in advanced HCC patients, it is important to develop a simple and rapid method to analyze the exposures of lenvatinib and its metabolites in human samples. Here, we established and validated a simple and rapid method for determining lenvatinib and its three major metabolites, descyclopropyl lenvatinib (M1), O‐demethyl lenvatinib hydrochloride (M2), and lenvatinib N‐Oxide (M3) by liquid chromatography‐tandem mass spectrometry method. Lenvatinib and its main metabolites were separated on an X‐Terra RP18 column (50 × 2.1 mm, 3.5 µm) at 35°C within 3 min, and the analytes were isocratically eluted with the mobile phase of methanol–water (10:90, v/v) containing 0.1% of formic acid at a flow rate of 0.15 mL/min. The calibration range was 1–1000 ng/mL for lenvatinib, while 0.1–100 ng/mL for M1–M3 under positive electrospray ionization mode. The inter‐ and intra‐batch precisions and accuracy were acceptable for lenvatinib and its metabolites. This method was successfully applied to measure lenvatinib and its metabolites in plasma samples from HCC patients, which provides a robust tool for pharmacokinetic–pharmacodynamics studies of lenvatinib.
Differential impacts of pH on growth, physiology, and elemental stoichiometry across three coccolithophore species
Authors: Chauhan Nishant , Rickaby Rosalind E. M. 
Published: December 2024

AbstractCoccolithophores are pivotal players in ocean biogeochemistry, yet the impact of changing pH on the physiology of different species remains unclear as there has been a dominant focus on Gephyrocapsa huxleyi. Meta‐analyses of existing experimental data are challenging due to the differences in multidimensional culture conditions. This study investigated the response of three species—Gephyrocapsa huxleyi, Coccolithus braarudii, and Chrysotila carterae—under varying CO2 conditions (via pH). The sensitivity to pH differed between species, but all species showed reduced growth rates under the highest CO2 (lowest pH) treatment possibly due to high [H+]‐related inhibition. Low pH impacted cellular physiology and elemental stoichiometry, while the impact of high pH was less adverse. The changes in elemental production induced by low pH could exert a negative influence on the contribution of coccolithophores to nutrient and carbon export, especially for biogeochemically relevant open‐ocean species. pH also affected coccolith formation, especially in C. braarudii, through CO2 limitation at high pH and low calcite saturation state at low pH. Contrasting species‐specific pH sensitivities highlighted the potential for species like G. huxleyi to further outperform others like C. braarudii in an acidic ocean. Literature synthesis showed that coccolithophores show a broad CO2 optimum, although growth rates and particulate inorganic carbon to particulate organic carbon ratios consistently declined with increasing CO2. Strain‐specific CO2 optima partly contributed to the variability within responses of individual species, giving the misleading perception of a broad species‐level CO2 optimum. Strain‐specific optima exist possibly due to their adaptation to carbonate chemistry conditions at the place of origin.
Patterns of siderophore production and utilization at Station ALOHA from the surface to mesopelagic waters
Authors: Bundy Randelle M. , Manck Lauren E. , Repeta Daniel J. , Church Matthew J. , Hawco Nicholas J. , Boiteau Rene M. , Park Jiwoon , DeLong Edward F. , Saito Mak A. 
Published: December 2024

AbstractThe North Pacific subtropical gyre is a globally important contributor to carbon uptake despite being a persistently oligotrophic ecosystem. Supply of the micronutrient iron to the upper ocean varies seasonally to episodically, and when coupled with rapid biological consumption, results in low iron concentrations. In this study, we examined changes in iron uptake rates, along with siderophore concentrations and biosynthesis potential at Station ALOHA across time (2013–2016) and depth (surface to 500 m) to observe changes in iron acquisition and internal cycling by the microbial community. The genetic potential for siderophore biosynthesis was widespread throughout the upper water column, and biosynthetic gene clusters peaked in spring and summer along with siderophore concentrations, suggesting changes in nutrient delivery, primary production, and carbon export seasonally impact iron acquisition. Dissolved iron turnover times, calculated from iron‐amended experiments in surface (15 m) and mesopelagic (300 m) waters, ranged from 9 to 252 d. The shortest average turnover times at both depths were associated with inorganic iron additions (14  9 d) and the longest with iron bound to strong siderophores (148  225 d). Uptake rates of siderophore‐bound iron were faster in mesopelagic waters than in the surface, leading to high Fe : C uptake ratios of heterotrophic bacteria in the upper mesopelagic. The rapid cycling and high demand for iron at 300 m suggest differences in microbial metabolism and iron acquisition in the mesopelagic compared to surface waters. Together, changes in siderophore production and consumption over the seasonal cycle suggest organic carbon availability impacts iron cycling at Station ALOHA.
Photoperiods and light differentially influence growth and potential niches of phycocyanin‐ and phycoerythrin‐rich picocyanobacteria
Authors: Śliwińska‐Wilczewska Sylwia , Konik Marta , Savoie Mireille , Aguilera Anabella , Omar Naaman M. , Campbell Douglas A. 
Published: December 2024

AbstractStrains from the picocyanobacteria genus Synechococcus are currently found across a wide range of photoperiods and photosynthetically active radiation. Future scenarios now forecast range expansions of marine Synechococcus into new photic regimes. We found that strains of temperate, coastal phycocyanin‐rich and phycoerythrin‐rich Synechococcus grew fastest under moderate photosynthetically active radiation, and a 24‐h photoperiod, despite a cumulative diel photon dose equivalent to conditions where growth was slower, under higher light and shorter photoperiods. Under optimal conditions, a phycoerythrin‐rich Synechococcus strain achieved a highest recorded cyanobacterial chlorophyll‐specific exponential growth rate (μ) of 4.5 d−1. Two phycoerythrin‐rich strains demonstrated wider ability to modulate light capture capacity, whereas two phycocyanin‐rich strains showed less change in light capture across increasing cumulative diel photon dose. All four coastal strains showed a decrease of effective absorption cross‐section for photosystem II photochemistry, vs. increasing cumulative diel photosynthetically active radiation doses. Within each strain, μ showed consistent, saturating responses to increasing cumulative diel photosystem II electron flux, with more variations in responses of μ to cumulative photosynthetically usable radiation. As photoperiod opportunists, coastal picocyanobacteria show potential to expand into longer photic regimes as higher latitudes warm.
Shifting phenology as a key driver of shelf zooplankton population variability
Authors: Honda Isabel A. , Ji Rubao , Britten Gregory L. , Thompson Cameron , Solow Andrew R. , Zang Zhengchen , Runge Jeffrey A. 
Published: December 2024

AbstractThe timing of biological events, known as phenology, plays a key role in shaping ecosystem dynamics, and climate change can significantly alter these timings. The Gulf of Maine on the Northeast U.S. Shelf is vulnerable to warming temperatures and other climate impacts, which could affect the distribution and production of plankton species sensitive to phenological shifts. In this study, we apply a novel data‐driven modeling approach to long‐term datasets to understand the population variability of Calanus finmarchicus, a lipid‐rich copepod that is fundamental to the Gulf of Maine food web. Our results reveal how phenology impacts the complex intermingling of top‐down and bottom‐up controls. We find that early initiation of the annual phytoplankton bloom prompts an early start to the reproductive season for populations of C. finmarchicus in the inner Gulf of Maine, resulting in high spring abundance. This spring condition appears to be conducive to enhanced predation pressure later in the season, consequently resulting in overall low C. finmarchicus abundance in the fall. These biologically controlled dynamics are less pronounced in the outer Gulf of Maine, where water exchanges near the boundary have a greater influence. Our analysis augments existing hypotheses in fisheries oceanography and classical ecological theory by considering unique plankton life‐history characteristics and shelf sea dynamics, offering new insights into the biological factors driving C. finmarchicus variability.
Improving Perception Threshold Tracking for Rapid Evaluation of Diabetic Peripheral Neuropathy
Authors: Klit Frederik Østergaard , Bollerslev Vitus Milver , Borbjerg Mette Krabsmark , Røikjer Johan , Ejskjær Niels , Mørch Carsten Dahl 
Published: December 2024

ABSTRACTIntroduction/AimsAccurate assessment of diabetic peripheral neuropathy (DPN) is essential to prevent further complications, yet current methods have limitations. Perception threshold tracking (PTT) offers promise as a novel approach for rapid evaluation of both large and small fiber dysfunction. To enhance efficiency, this study explores the Psi method compared to the Method of Limits (MoL) in estimating perception thresholds. The aim is to assess agreement, uncertainty, and the number of stimuli required for adequate estimation by each method.MethodsForty‐three participants with diabetes underwent an estimated sural nerve conduction study, quantitative sensory testing, vibration perception threshold testing, and PTT. PTT utilized both patch and pin electrode configurations to activate large and small fibers, respectively. The uncertainty of perception thresholds was estimated as the inverse slope of the psychometric functions.ResultsPerception thresholds were significantly higher for the patch electrode (2.5 [2.1–3.0] mA) compared to the pin electrode (0.71 [0.56–0.91] mA; rmANOVA, p < 0.001). Bland–Altman analysis revealed a non‐significant 3.3% bias between the methods, but wide limits of agreement (−42%–84%). Uncertainty was lower for the Psi method (0.80 [0.58;1.11] mA) compared to MoL (2.0 [1.2;2.9] mA; rmANOVA, p < 0.005). The Psi method achieved acceptable perception threshold estimation with only 30 stimuli.DiscussionAt the group level, the MoL and Psi methods produced similar perception thresholds. However, the Psi method required fewer stimuli and yielded less uncertainty in perception threshold estimation compared to MoL. Future studies should prioritize the Psi method for its efficiency and reliability.
Recent Advances in Multifunctional Naturally Derived Bioadhesives for Tissue Engineering and Wound Management
Authors: Jafari Aliakbar , Al‐Ostaz Ahmed , Nouranian Sasan 
Published: December 2024

ABSTRACTRecent advancements in naturally derived bioadhesives have transformed their application across diverse medical fields, including tissue engineering, wound management, and surgery. This review focuses on the innovative development and multifunctional nature of these bioadhesives, particularly emphasizing their role in enhancing adhesion performance in wet environments and optimizing mechanical properties for use in dynamic tissues. Key areas covered include the chemical and physical mechanisms of adhesion, the incorporation of multi‐adhesion strategies that combine covalent and non‐covalent bonding, and bioinspired designs mimicking natural adhesives such as those of barnacles and mussels. Additionally, the review discusses emerging applications of bioadhesives in the regeneration of musculoskeletal, cardiac, neural, and ocular tissues, highlighting the potential for bioadhesive‐based therapies in complex biological settings. Despite substantial progress, challenges such as scaling lab‐based innovations for clinical use and overcoming environmental and mechanical constraints remain critical. Ongoing research in bioadhesive technologies aims to bridge these gaps, promising significant improvements in medical adhesives tailored for diverse therapeutic needs.
Prenatal‐Postnatal Outcomes and Prognostic Risk Factors of Fetal Volvulus: Analysis of 26 Cases
Authors: Taşdemir Ümit , Eyisoy Ömer Gökhan , Gezer Murad , Celayir Ayşenur , Eriç Özdemir Mucize , Demirci Oya 
Published: December 2024

ABSTRACTObjectiveThe aim of the current study was to reveal ultrasonographic and clinical features, prenatal–postnatal outcomes and prognostic risk factors of fetal volvulus.MethodThis retrospective study evaluated all cases of fetal volvulus diagnosed between 2018 and 2024 at the Perinatology center of Zeynep Kamil Women and Children Diseases Training and Research Hospital. In the vast majority of cases, the pediatric surgery team confirmed the conclusive diagnosis of volvulus during the postnatal period. The cohort was divided into two groups, the “survivor” and “deceased”, in order to compare the outcome and to evaluate factors determining the outcome.ResultA total of 26 cases of fetal volvulus were followed up in our perinatology center which were confirmed by postnatal pediatric surgery or autopsy. Termination of pregnancy in two cases and intrauterine fetal death in one case were observed. Twenty‐three cases reached live birth. Preterm labor, fetal growth restriction, ascites and decreased intestinal peristalsis were significantly more common in the deceased group. Neonatal death in five cases (19.2%), infant death in four cases (15.3%) and short gut syndrome in three cases (11.5%) were long term outcomes.ConclusionOverall mortality rate may increase when fetal growth restriction, ascites, and decreased intestinal peristalsis are present with volvulus.
Socio‐Economic Inequalities in Beliefs About Cancer and its Causes: Evidence From two Population Surveys
Authors: Galicia Pacheco Sergio Iván , Catena Andrés , Sánchez María José , Rueda Maria del Mar , Aljarilla Sánchez Lucas , Costas Laura , Garrido Dunia , Garcia‐Retamero Rocio , Espina Carolina , Rodríguez‐Barranco Miguel , Petrova Dafina 
Published: December 2024

ABSTRACTObjectivePeople's beliefs about cancer can affect the actions they take to prevent and detect the disease. We investigated socio‐economic inequalities in beliefs about cancer and its causes in the general population.MethodsWe analyzed data from the representative probabilistic Spanish Oncobarometer survey (N = 4769, 2020) and the non‐probabilistic weight‐corrected Spanish Cancer Awareness Survey (N = 1029, 2022). Beliefs about cancer, recognition of cancer symptoms, and recognition of risk factors were measured with the Awareness and Beliefs about Cancer questionnaire. Endorsement of mythical causes was measured with the CAM‐Mythical Causes questionnaire. The effects of socio‐economic status (SES) were investigated in multiple regression analyses adjusted for age, sex, and personal and family cancer history.ResultsIndividuals with lower SES were more likely to endorse pessimistic beliefs (e.g., “cancer is a death sentence”), and less likely to endorse optimistic beliefs about cancer (e.g., “people with cancer continue with normal activities”). Individuals with lower SES also recognized fewer cancer symptoms and risk factors and endorsed more mythical causes of cancer. The gap in knowledge regarding cancer causes was wider among people with low SES, who were more likely to endorse several mythical causes than some established risk factors included in cancer prevention recommendations.ConclusionsSocio‐economic inequalities in beliefs about cancer are robust and multidimensional and indicate worse preparedness to act against the disease among lower socio‐economic groups. Differences in beliefs about disease outcomes and causes are likely one of the multiple contributors to cancer disparities and should be targeted and monitored in prevention efforts.
Comparison of the Incidence and Magnitude of Hyponatremia Among Patients With Poststroke Depression Receiving Either Escitalopram or Sertraline
Authors: Naseralallah Lina , Noureddine Zahra , Koryash Somaya 
Published: December 2024

ABSTRACTDepression is the most frequent psychiatric condition experienced in stroke survivors. Selective serotonin reuptake inhibitors (SSRIs) are frequently used as first‐line antidepressants; however, they have been strongly associated with hyponatremia which, in poststroke patients, can worsen outcomes. This study aims to determine and compare the incidence and magnitude of hyponatremia and potential risk factors in patients receiving either escitalopram or sertraline for the management of poststroke depression (PSD). A retrospective observational study involving all hospitalized patients who received either escitalopram or sertraline for the treatment of PSD. Electronic medical records were reviewed over a 5‐year period with data collected on various demographic, laboratory, comorbidity, and medication‐related variables. Data were analyzed using multivariate logistic regression. A total of 401 patients met the inclusion criteria. Overall, 36.7% of patients experienced hyponatremia, with 67 (38.3%) cases in patients receiving escitalopram and 76 (33.6%) in sertraline group. The median drop in sodium level from baseline was 5 mmol/L in both groups; with the majority of cases being of mild nature (73.1% and 69.7% for escitalopram and sertraline, respectively). Findings from the multivariate logistic regression did not yield a model with significant association (p = 0.353). Escitalopram and sertraline were both associated with an increased risk of hyponatremia in poststroke patients, with most cases being mild. There was no significant difference between treatment arms regarding the incidence or magnitude of hyponatremia. Caution should be exercised when prescribing escitalopram or sertraline.
Symbiotic bacteria play crucial roles in a herbivorous mite host suitability
Authors: Hu Yue , Wang Mei‐Ling , Yang Ruo‐Lan , Shao Zi‐Kai , Du Yun‐Hao , Kang Yi , Zhu Yu‐Xi , Xue Xiao‐Feng 
Published: December 2024

AbstractBACKGROUNDThe tomato russet mite (TRM), Aculops lycopersici, is a strictly herbivorous and economically significant pest that infests Solanaceae plants, but its host suitability varies, showing high performance on tomatoes. Although symbiotic bacteria have been suggested to play crucial roles in the host adaptation of herbivores, their effects on TRM remain unclear.RESULTSIn this study, using next generation high‐throughput sequencing of the bacterial 16S rRNA data, we identified the bacterial diversity and community composition of TRM feeding on tomato, eggplant, and chili. Our results show no significant difference in the bacterial community composition of TRM across three host plants. However, the relative density of Escherichia coli (TRM_Escherichia) showed 9.36‐fold higher on tomato than on eggplant and chili. These results align with the observed TRM performance among three host plants. When TRM_Escherichia was reduced using antibiotics, the treated TRM decreased the population density on tomato. However, when we transferred TRM from eggplant to tomato, the population density of TRM increased, coinciding with an increase of the TRM_Escherichia density. These results indicate that TRM_Escherichia may affect the host suitability of TRM. Our fluorescence in situ hybridization (FISH) results further showed that TRM_Escherichia is primarily distributed in the salivary glands. Metagenomic data results suggest that TRM_Escherichia functions in food digestion and energy metabolism.CONCLUSIONWe provided the first comprehensive analysis of TRM bacterial communities. Our findings demonstrate that the symbiotic bacterium TRM_Escherichia may play crucial roles in the suitability of TRM feeding on different Solanaceae hosts. © 2024 Society of Chemical Industry.
Study of fog dissipation in an internal boundary layer on Sable Island
Authors: Bardoel Stef L. , Hoch Sebastian , Ruiz‐Plancarte Jesus , Lenain Luc , Gultepe Ismail , Grachev Andrey A. , Gaberšek Saša , Wang Qing , Fernando Harindra J. S. 
Published: December 2024

AbstractAn interesting fog dissipation event was observed during the Fog and Turbulence Interactions in the Marine Atmosphere (FATIMA) Grand Banks field campaign, where a fog‐free region appeared immediately downstream of Sable Island as fog advected past it. This fog‐free region was predicted a priori by a high‐resolution numerical model that guided intensive operational periods of the FATIMA campaign, and its presence was adumbrated by GOES satellite observations. A comprehensive set of field observations shows that this fog‐free layer was due to the development of a (daytime) thermal internal boundary layer (IBL) that grew with distance from the leading shore line. The net incoming radiation following sunrise led to an increased air temperature and decreased relative humidity close to the ground, thus dissipating fog over the island. The height of the thermal IBL, as identified by the thickness of the superadiabatic layer, was found to be consistent with several available theoretical IBL formulae.