bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengembangan Bahan Ajar Fisika Dengan Model Pembelajaran Inkuiri Terbimbimbing Untuk Melatihkan Keterampilan Proses Sains
Authors: Fadhila Firdha Nur , Arifuddin Muhammad , Miriam Sarah 
Published: August 2019

Bahan ajar fisika fluida dinamis melalui model pembelajaran inkuiri terbimbing belum sesuai dengan yang diharapkan. Hal ini berdampak pada rendahnya Keterampilan Proses Sains dan hasil belajar siswa. Penelitian ini bertujuan menghasilkan dan mendeskripsikan kelayakan bahan ajar menggunakan model inkuiri terbimbing untuk melatihkan keterampilan proses sains. Tujuan khusus penelitian mendeskripsikan: (1) validitas bahan ajar dikembangkan berdasarkan uji validasi (2) kepraktisan bahan ajar yang dikembangkan berdasarkan keterlaksanaan rencana pelaksanaan pembelajaran, (3) efektivitas bahan ajar yang dikembangkan berdasarkan hasil belajar kognitif siswa dan (4) ketercapaian KPS dikembangkan berdasarkan lembar pengamatan KPS. Jenis penelitian ini, yaitu penelitian dan pengembangan yang mengacu pada model pengembangan Analysis, Design, Development, Implementation, Evaluation. Subjek uji coba penelitian ini adalah 20 siswa kelas XI IPA 2 SMA KORPRI Banjarmasin. Data diperoleh melalui lembar validasi bahan ajar, lembar pengamatan keterlaksanaan Rencana Pelaksanaan Pembelajaran, lembar tes hasil belajar dan lembar pengamatan KPS. Teknik analisis data meliputi, validitas, reliabilitas dan uji N-gain. Hasil penelitian menunjukkan bahwa (1) validitas bahan ajar yang dikembangkan adalah valid, (2) kepraktisan bahan ajar adalah terlaksana sangat baik, (3) efektivitas bahan ajar adalah sedang dan (4) ketercapaian KPS adalah sangat baik. Diperoleh simpulan bahwa bahan ajar menggunakan model inkuiri terbimbing pada pokok bahasan fluida dinamis layak untuk digunakan.
Meningkatkan Keterampilan Prosedural dan Hasil Belajar Dengan Metode Pemecahan Masalah Melalui Pengajaran Langsung
Authors: Hanisa Nur Maya , Arifuddin Muhammad , Miriam Sarah 
Published: August 2019

Keterampilan prosedural dan hasil belajar yang belum sesuai harapan.Penelitian bertujuan untuk mendeskripsikan peningkatan keterampilan prosedural dan hasil belajar siswamelalui pengajaran langsung dengan menggunakan metode pemecahan masalah. Tujuan khusus penelitian ini, yaitu mendeskripsikan keterlaksanaan RPP, keterampilan prosedural, dan hasil belajar siswa. Jenis penelitian yang digunakan yaitu penelitian tindakan kelas menggunkan model Kemmis dan Mc. Taggart. Subjek penelitian ini yaitu siswa kelas X MIPA 4 yang berjumlah 30 orang. Data diperoleh dari tes hasil belajar dan observasi. Hasil penelitian ini menunjukkan bahwa 1) keterlaksanaan RPP pada siklus I dan II berkategori sangat baik, 2) keterampilan prosedural pada siklus I berkategori cukup terampil dan pada siklus II berkategori terampil, dan 3) hasil belajar juga meningkat dengan persentase ketuntasan secara klasikal pada siklus I sebesar 46,67% dan pada siklus II sebesar 80%. Disimpulkan bahwa dengan menggunakan metode pemecahan masalah melalui pengajaran langsung keterampilan prosedural dan hasil belajar dapat meningkat.
Meningkatkan Minat dan Hasil Belajar Peserta Didik Menggunakan Model Pembelajaran Inkuiri Terbimbing
Authors: Karlina Karlina , Susilowati Eko , Miriam Sarah 
Published: August 2019

Minat dan hasil belajar peserta didik kelas XI MIPA 3 SMA Negeri 6 Banjarmasin masih rendah. Oleh karena itu, dilakukan upaya untuk meningkatkan minat dan hasil belajar didik. Penelitian ini bertujuan untuk mendeskripsikan keterlaksanaan RPP, minat , dan hasil belajar. Subjek penelitian ini adalah peserta didik kelas XI MIPA 3 SMA Negeri 6 Banjarmasin tahun pelajaran 2018/2019 yang berjumlah 30 peserta didik. Jenis penelitian ini merupakan penelitian tindakan kelas model Kemmis dan Mc Taggart terdiri dari 2 siklus setiap siklus meliputi perencanaan, tindakan dan observasi, serta refleksi. Teknik pengumpulan data berupa observasi, tes, dan angket. Data dianalisis secara deskriptif kualitatif dan kuantitatif. Hasil penelitian siklus I dan siklus II yaitu: 1) Keterlaksanaan RPP secara keseluruhan memperoleh kriteria sangat baik, 2) Minat peserta didik dengan menggunakan model pembelajaran inkuiri terbimbing meningkat dari 74 dengan kriteria baik menjadi 82 dengan kriteria sangat baik, dan 3) Hasil belajar peserta didik meningkat dengan ketuntasan klasikal sebesar 23,3% menjadi sebesar 80%. Diperoleh simpulan bahwa model pembelajaran inkuiri terbimbing dapat meningkatkan minat dan hasil belajar peserta didik kelas XI MIPA 3 SMA Negeri 6 Banjarmasin.
ESTÁGIO SUPERVISIONADO EAD: mesmas diretrizes, novas conexões
Authors: Zacariotti Marluce , Pinho Maria José de 
Published: August 2019

Este artigo descreve a implantação do estágio curricular supervisionado do Curso de Licenciatura em Biologia na modalidade a distância da Universidade Federal do Tocantins (UFT). Discute teorias de origem positivista do ensino tradicional, contrapondo-as a propostas emergentes do ensino a distância (EaD), e reflete sobre a interação entre (e com) os alunos estagiários da UFT na plataforma Moodle. Ressaltam-se as percepções sobre essas interações, na perspectiva do conhecimento compartilhado e de processos reflexivos mediados pelas tecnologias. Partindo do princípio de que as diretrizes, tanto no estágio curricular presencial quanto no a distância, são baseadas no mesmo decreto 87.497, busca-se levantar questões que demarquem diferenças ou aproximações entre ambas as propostas.

PALAVRAS-CHAVE: plataforma moodle; estágio supervisionado; educação a distância.

ABSTRACT
This article describes the implantation of the supervised training in undergraduate course of biology in the distance modality of the Federal University of Tocantins (UFT). It discusses positivistic line theories of traditional education, counterposing them with emerging proposals of e-learning, and reflects on the interaction between (and with) students trainees of UFT in the Moodle platform. The perceptions of these interactions are emphasized, In the perspective of shared knowledge and reflective processes mediated by technologies. Assuming that the guidelines, both online and on-site supervised academic training programs, are based on the same decree 87,497, it seeks to raise issues that demark differences or approximations between both proposals.

KEYWORDS: moodle platform; supervised training; distance e-learning.

RESUMEN
En este artículo se describe la implementación de los estrenamientos curriculares supervisionados en el curso de licenciatura en Biología a distancia de la Universidad Federal de Tocantins (UFT). Discute las teorías de orientación positivista de la enseñanza tradicional, comparándolos con las propuestas que surgen de la enseñanza a distancia y reflexiona sobre la interacción entre (y con) los estudiantes de la UFT en la plataforma Moodle. Se resaltam las percepciones acerca de estas interacciones, desde la perspectiva de compartir conocimientos y procesos reflexivos mediados por la tecnología. Partiendo del principio de que las directrices curriculares, tanto en entrenamiento curricular presencial quanto a distancia, se basan en el mismo decreto 87,497, tratamos de plantear cuestiones que señalen las diferencias o aproximaciones entre ambas propuestas.

PALABRAS CLAVE: Plataforma moodle; entrenamiento supervisionado; educación a distancia.
DESAFIOS PARA IMPLEMENTAÇÃO DAS TECNOLOGIAS DIGITAIS NO AMBIENTE ACADÊMICO
Authors: Martins José Lauro , Jesus Layane Mota de Souza de , Chaves Arlane Silva Carvalho , Takahagassi Patricia Delmiro de Sousa , Sousa Marcia Pessoa de 
Published: August 2019

As Tecnologias Digitais (TD), são recursos importantes para o processo de ensino aprendizagem e favorecem o trabalho docente, porém estes ainda enfrentam alguns desafios quanto a sua utilização. Objetivou-se, realizar uma revisão bibliográfica dos principais desafios enfrentados pelos docentes para implementação das TD no ambiente acadêmico. Trata-se de uma pesquisa bibliográfica do tipo narrativa, onde foram selecionados 14 artigos, 02 dissertações, 01 monografias e 02 livros, publicados em português na base de dados da CAPES, no período de 2012 a 2017. Os principais desafios apontados são: resistência docente à mudança, ausência de autonomia docente frente ao uso das TD, ausência de capacitação para uso, bem como de políticas que favoreçam a apropriação crítica dos docentes quanto as tecnologias.

PALAVRAS-CHAVE: Tecnologias Digitais; Desafios; Docentes.

ABSTRACT
Digital Technologies (TD), are important resources for the process of teaching learning and favor the teaching work, but these still face some challenges as to their use. The objective was to carry out a bibliographic review of the main challenges faced by teachers for the implementation of TD in the academic environment. It is a bibliographical research of the narrative type, 14 articles, 02 dissertation, 01 monograph and 02 book were published, published in Portuguese in the CAPES database, from the period of 2012 to 2017. The main challenges are: teacher resistance to change, absence of teacher autonomy from the use of TD, lack of training for use, as well as policies that favor the critical appropriation of teachers regarding technologies.

KEYWORDS: Digital Technologies; Challenges; Docentes.

RESUMEN
Las Tecnologías Digitales (TD), son recursos importantes para el proceso de enseñanza aprendizaje y favorecen el trabajo docente, pero éstos todavía enfrentan algunos desafíos en cuanto a su utilización. El objetivo es realizar una revisión bibliográfica de los principales desafíos enfrentados por los docentes para la implementación de las TD en el ambiente académico. Se trata de una investigación bibliográfica del tipo narrativa, donde fueron seleccionados 14 artículos, 2 disertaciones, 1 monografía y 2 libros publicados en portugués en la base de datos de la CAPES, en el período de 2012 a 2017. Los principales desafíos señalados son: resistencia docente al cambio, ausencia de autonomía docente frente al uso de las TD, ausencia de capacitación para el uso de las TD, así como de políticas que favorezcan el pensamiento crítico de los docentes en cuanto a las tecnologías.

PALABRAS CLAVE: Tecnologías Digitales; Desafíos; Docentes.
Novel mutations of the SRF gene in Chinese sporadic conotruncal heart defect patients
Authors: Mengmeng Xu , Yuejuan Xu , Sun Chen , Yanan Lu , Fen Li , Kun Sun 
Published: August 2019

Abstract
Background Conotruncal heart defects (CTDs) are a group of congenital heart malformations that cause anomalies of cardiac outflow tracts. In the past few decades, many genes related to CTDs have been reported. Serum response factor (SRF) is a ubiquitous nuclear protein that acts as transcription factor, and SRF was found to be a critical factor in heart development and to be strongly expressed in the myocardium of the developing mouse and chicken hearts. The targeted inactivation of SRF during heart development leads to embryonic lethality and myocardial defects in mice.Results To illustrate the relationship between SRF and human heart defects, we screened SRF mutations in 527 CTD patients. Two novel mutations (p.G274D and p.G294C) of SRF were identified. Western blotting and real-time PCR showed that there were no obvious differences between the protein expression and mRNA transcription of mutants and wild-type SRF. A dual luciferase reporter assay showed that both SRF mutants (G274D and G294C) impaired SRF transcriptional activity at the SRF promoter and atrial natriuretic factor (ANF) promoter (p<0.05), additionally, the mutants displayed reduced synergism with GATA4.Conclusion These results suggest that SRF-p.G274D and SRF-p.G294C may have potential pathogenic effects.
Identification and Characterization of SNPs in released, landrace and wild accessions of Mungbean (Vigna radiata (L.) Wilczek) using whole genome re-sequencing
Authors: Bangar Pooja , Tyagi Neetu , Tiwari Bhavana , Kumar Sanjay , Barman Paramananda , Kumari Ratna , Gaikwad Ambika , Bhat KV , Chaudhury Ashok 
Published: August 2019

Abstract
Mungbean [Vigna radiata (L.) R. Wilczek var. radiata] is vital grain legume having nutritional and socio-economic importance, especially in the developing countries. We performed whole genome re-sequencing of three accessions representing the wild progenitor species, released and landrace of mungbean to identify SNPs with relevance to genetic relationships analyses. Approximately 9.3 million raw reads were obtained by using Ion Torrent PGM™ platform and more than 92% of the reads were mapped to the reference mungbean genome. We identified a total of 233,799 single nucleotide polymorphisms in relation to the reference genome (SNPs: 103,341 in wild, 93,078 in released and 37,380 in landrace accessions) and 9,544 insertions and deletions (InDels: 4,742 in wild, 3,608 in released and 1,194 in landrace accessions) in the coding and non-coding regions. In all accessions, genomic variants were unevenly distributed within and across the mungbean chromosomes. Among these 5,339; 4,739 and 1,795 SNPs were non-synonymous in 815, 790 and 317 genes of wild, released and landrace accessions, respectively. These polymorphisms might contribute to the variation in important pathways of genes for abiotic and biotic stress tolerance and important agronomic traits such as seed dormancy, flowering time and seed size in mungbean. Among the randomly selected SNPs, a selected subset was validated using Sanger sequencing technique. The genomic variations among mungbean wild, released and landrace accessions constitute a powerful tool to support genetic research and molecular breeding of mungbean.
Integrated analysis of miRNA-seq and mRNA-seq reveals immune related miRNA-mRNA regulation network in the DHAV-3-infected duckling liver
Authors: Wu Fengyao , Lu Fengying , Fan Xin , Chao Jin , Liu Chuanmin , Pan Qunxing , Sun Huawei , Zhang Xiaofei 
Published: August 2019

Abstract
Background: Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings is remain poorly understood. To elucidate the genetic regulatory network for miRNA-mRNA and the signaling pathways involved in DHAV-3 infection in ducklings, we conducted global miRNA and mRNA expression profiling of duckling liver tissues infected with lethal DHAV-3 using high-throughput sequencing.
Results: We found 156 differentially expressed miRNAs (DEMs) and 7717 differentially expressed miRNAs (DEGs) between mock-infected and DHAV-3-infected duckling livers. A total of 19,606 miRNA-mRNA pairs with negatively correlated expression patterns were identified in miRNA-mRNA networks constructed on the basis of these DEMs and DEGs. Moreover, immune-related pathways including the cytokine-cytokine receptor interaction, apoptosis, Toll-like receptor, Jak-STAT, and RIG-I-like receptor signaling pathway were significantly enriched through analyzing functions of mRNAs in the network in response to DHAV-3 infection. Besides, apl-miR-32-5p, apl-miR-125-5p, apl-miR-128-3p, apl-miR-460-5p, and novel-m0012-3p were identified as potential regulators in the immune-related signaling pathways during the DHAV-3 infection.
Conclusions: To our knowledge, this is the first report on integrated analysis of miRNA-seq and mRNA-seq in DHAV-3-infected ducklings. The results indicated the important roles of miRNAs in regulating immune response genes and revealed the immune related miRNA-mRNA regulation network in the DHAV-3-infected duckling liver. Our findings may provide valuable information to further investigate the roles of miRNAs and their target genes in DHAV-3 replication and pathogenesis; additionally, they may offer clues for further understanding host-virus interactions.
Explicating Practice Norms and Tensions Between Values in Resident Training in Family Medicine
Authors: Achkar Morhaf Al 
Published: August 2019

Abstract
Purpose Residency programs have the intricate and complex role of training health care providers. But little is known about what residents and attendings consider norms of practice or the tensions among different values residents are expected to uphold. Thus, dialogical and reflective frameworks are being explored for resident learning. Methods This study examined the use of facilitated conversations with groups of residents and attending physicians while reviewing video-recorded resident–patient interactions. The conversations were recorded, transcribed, and qualitatively analyzed.Results A total of 24 residents and 10 attendings participated in conversations while separately and in parallel groups reviewing 15 resident sessions. Residents explicated the norms of practice and evaluated their performance, which often agreed with those of attending physicians in calling out important learning opportunities. When disagreement occurred, residents’ explications of their reasoning were often relevant and, via reflection and dialogue, helped clarify intentions that were not apparent in the videos. Residents and attendings both often judged actions on more than one domain of value. For instance, if a resident addressed problems, built relationships in a timely manner, and acted autonomously without jeopardizing the quality of care, she satisfactorily performed her duty. Conclusions Practice norms and value struggles were addressed by participants during reviews, which provided a promising framework for learning and assessment. Also, the non-hierarchical structure opened space to acknowledge a diversity of positions and for tensions among values to be explicated.
Does Video Laryngoscopy or Direct Laryngoscopy affect first pass success rates for intubation among Attending and Non-Attending Emergency Physician in the Emergency Department?
Authors: Wan Paul Weng , Diana Zakaria Nur , Ching Seow Gek , Evelyn Wong 
Published: August 2019

Abstract
Background: To our knowledge, there has been no study comparing intubation characteristics between attending and non-attending Emergency Physicians in South-East Asia. We aim to identify whether the use of Direct Laryngoscopy (DL) compared to Video Laryngoscopy (VL) affects first pass success rates between Attending Emergency Physicians (AEP) and Non-Attending Emergency Physicians (NAEP). Materials and Methods: Retrospective analysis of data from 2009 to 2016 in an existing airway registry managed by and academic Emergency Department in Singapore. Primary outcome is first pass success intubation rate. The secondary outcome was first pass success rate for difficult intubations. Difficult intubations were defined as LEMON score of more than 1 or more than 1 attempt at intubation. Results: There were 2909 intubation carried out by emergency physicians in the Emergency Department from 2009 to 2016. AEP conducted 1748 intubations while NAEP conducted 1161 intubations. The first pass success rates for AEP was 84.2% while that for NAEP was 67.4%. 86.2% of intubations by AEP were done with a direct laryngoscope. 89.0% of the intubations by NAEP were done with a direct laryngoscope. 18.9% of intubations by the AEP were difficult compared to 35.2% by the NAEP (p<0.01 95% C.I 13.0%-19.6%). First pass success rate with VL was lower than DL for all intubations (OR 0.66, 95% C.I 0.51-0.84). In the subgroup of difficult intubations, VL did not improve first pass success rate among AEP (OR 0.77, 95% C.I 0.38-1.58) but it did for NAEP (OR 2.46, 95% C.I 0.94-6.45). Conclusion: Our study showed that VL has a poorer first pass success rate for all intubations in general. However, specifically for difficult intubations, VL is associated with improved first pass success rates among NAEP.
Transcriptomic dynamics changes in development of carmine radish (Raphanus sativus L.) fleshy roots using RNA-seq method
Authors: Gao Jian , Luo Mao , Liu Yi , Chen Fabo , Peng Hua , Li Wenbo 
Published: August 2019

Abstract
Radish ( Raphanus sativus L.), belonging to biennial root vegetable crop of Brassicaceae family, is an economically important vegetable crop with an edible taproot. Recently, most of differential expressed genes associating with anthocyanin biosynthesis have been identified in most of important fruit crops. However, transcriptome analysis of anthocyanin biosynthesis and expression of anthocyanin biosynthesis related genes in ‘Hongxin’ radish have not been fully investigated. Here, based on results from HPLC analysis, young fleshy roots obtained from the dynamics development stage of fleshy roots in carmine radish ‘Hongxin 1’ was used for RNA-Seq, including fleshy roots from seedling stage (SS), initial expansion (IE), full-expansion (FE), bolting stage (BS), initial flowering stage (IFS); full-bloom stage (FBS) and podding stage (PS). Subsequently, the putative candidate genes involved in the dynamics development stage of fleshy roots in carmine radish were identified. After that, DGE (differential gene expression) profile analysis was used to identify the pupative transcripts, compared with fleshy roots from seedling stage (SS). In addition, co-modulated DEGs (Common DEGs in the dynamic growing stages of fleshyroot in carmine radish) were also identified, from which most DGEs were more likely to participate in anthocyanin biosynthesis, including two transcription factors RsMYB and Rs RZFP . In addition, some related proteins e.g. RsCHS , RsDFR , RsANS , RsF’3H , RsF3GGT1 , Rs3AT1 , glutathione S-transferase F12, RsUFGT78D2-like and RsUDGT-75C1-like were significantly contributed to the regulatory mechanism during anthocyanin synthesis in the development stage of fleshy roots. Furthermore, GO terms comprised of “anthocyanin-containing compound biosynthetic process” and “anthocyanin-containing compound metabolic process” were commonly overrepresented in the other dynamics growing stages of fleshy roots after initial expansion of fleshy roots. Moreover, these results indicated that five significantly enrichment pathways of DEG were identified for the dynamics growing stages of fleshy roots in carmine radish, including Flavonoid biosynthesis, Flavone and flavonol biosynthesis, Diterpenoid biosynthesis, Anthocyanin biosynthesis, as well as Benzoxazinoid biosynthesis. These results will expand our understanding of complex molecular mechanism of the putative candidate genes involved in the dynamics development stage of fleshyroot in carmine radish.