bluuebunny/crossref_metadata_2025_split huggingface dataset:
L'invitation au voyage
Authors: Peyvel Emmanuelle 
Published: February 2018

Comment devenons-nous touriste ? Peut-on repenser la mondialisation du tourisme au regard des arts de faire de celles et ceux qui l’inventent aujourd’hui ? Cet ouvrage se propose de répondre à partir du Việt Nam, pays encore pauvre qui enregistre une croissance significative du tourisme domestique depuis plus de vingt ans.
Les observations in situ et les matériaux biographiques construits depuis 2005 à travers le pays permettent de comprendre comment les Vietnamiens apprennent aujourd’hui à voyager dans leur pays, où les richesses comme les inégalités s’accroissent depuis le Đổi Mới. Une analyse critique de la gestion politique et normative du tourisme domestique, d’une part par l’État vietnamien et d’autre part par les institutions mondiales du tourisme, complète ce travail de terrain, afin de traduire les rapports complexes que ces touristes entretiennent avec les systèmes de contraintes au sein desquels ils se meuvent. La colonisation comme le socialisme sont ici compris non seulement comme des périodes historiques, mais aussi comme un ensemble d’organisations, de valeurs, d’acteurs et de pratiques qui sont aujourd’hui mobilisés comme ressources afin de construire des mobilités touristiques. L’étude géographique qui en est faite révèle l’inventivité de leurs apparitions, réappropriations et multiplications, selon des hybridations et des circulations, parfois conflictuelles, de pratiques et d’imaginaires.
Ce faisant, c’est une vision décentrée de la mondialisation touristique que propose cet ouvrage, permettant de mieux comprendre toute la complexité avec laquelle se reconfigurent les rapports de pouvoir au sein des sociétés postcoloniales.
Rechtsgeldigheid van het relocatiebesluit en de betekenis van het solidariteitsbeginsel in het EU-asielbeleid
Authors: Brouwer E.R. 
Published: February 2018

Rechtsgeldigheid van het relocatiebesluit en de betekenis van het solidariteitsbeginsel in het EU-asielbeleid
Op 6 september 2017 verwierp het Hof van Justitie het beroep van Hongarije en Slowakije tot nietigverklaring van het Raadsbesluit van 22 september 2015. In dit besluit werd een door de Europese Commissie voorgesteld schema vastgesteld voor de relocatie of herplaatsing van asielzoekers vanuit Griekenland en Italië naar andere lidstaten van de Unie. Het Hof van Justitie verwerpt alle argumenten van de twee lidstaten waarmee de rechtsgeldigheid van het besluit werd betwist. In de uitspraak bevestigt het Hof van Justitie niet alleen de geldigheid van het relocatiebesluit met een beroep op het solidariteitsbeginsel, maar onderstreept ook het belang van een effectief rechtsmiddel tegen een herplaatsingsbesluit en de naleving van het non-discriminatiebeginsel.HvJ 6 september 2017, gevoegde zaken C-643/15 en C-647/15, Slowaakse Republiek en Hongarije/Raad van de Europese Unie, ECLI:EU:C:2017:631
Palladium‐Catalyzed Triarylation of sp3 C−H Bonds in Heteroarylmethanes: Synthesis of Triaryl(heteroaryl)methanes
Authors: Zhang Shuguang , Hu Bowen , Zheng Zhipeng , Walsh Patrick J. 
Published: February 2018

AbstractA straightforward method for the palladium‐catalyzed triarylation of heteroarylmethanes at the methyl group has been developed. The reaction works with a variety of aryl halides, enabling the rapid synthesis of triaryl(heteroaryl)methanes in moderate to excellent yields.magnified image
Flai (fludarabine, cytarabine, idarubicin) plus low‐dose Gemtuzumab Ozogamicin as induction therapy in CD33‐positive AML: Final results and long term outcome of a phase II multicenter clinical trial
Authors: Candoni Anna , Papayannidis Cristina , Martinelli Giovanni , Simeone Erica , Gottardi Michele , Iacobucci Ilaria , Gherlinzoni Filippo , Visani Giuseppe , Baccarani Michele , Fanin Renato 
Published: February 2018

AbstractThe aim of this prospective clinical trial was to evaluate the efficacy and safety of a combination of Gemtuzumab‐Ozogamicin (GO) and FLAI scheme (fludarabine, cytarabine, idarubicin) as a first‐line therapy in CD33 positive AML. We treated 130 patients, aged <65, with a median age of 52 years. FLAI‐GO induction regimen included fludarabine (30 mg/sqm) and cytarabine (2 g/sqm) on days 1–5; idarubicin (10 mg/sqm) on days 1, 3, and 5; and GO (3 mg/sqm) on day 6. SCT was planned for all high‐risk AML patients, after consolidation with intermediate doses of cytarabine and idarubicin and a high dose of cytarabine. CD33 expression exceeded 20% in all cases. Primary endpoints of the study included feasibility, overall response rate (ORR) and toxicity. Secondary endpoints included the evaluation of MRD by WT1 expression, feasibility and outcome of consolidation with SCT, overall survival (OS) and disease‐free survival (DFS).After induction with FLAI‐GO, complete remission (CR) rate was 82%. Four patients achieved partial remission (PR) and 12% were resistant (ORR 85%); death during induction (DDI) was 3%. The hematological and extra hematological toxicity of FLAI‐GO was manageable; 45% of patients experienced transient and reversible GO infusion related adverse events. In the setting of patients who achieved a cytological CR after FLAI‐GO, the mean of WT1 copies dropped from 8337±9936 copies/104ABL (diagnosis) to 182 ± 436 copies after induction therapy (p = 0.0001) showing a very good disease debulking. After a median follow‐up of 54 months, 67/130 (52%) patients were alive. The probability of 1, 2, and 5‐year OS was 80%, 63%, and 52%, respectively. The probability of 1, 2, and 5‐year DFS was 77%, 58%, and 52%, respectively. Allogeneic and autologous SCT was performed in 60 (46%) and 23 (18%) patients, respectively.In summary, the final results of this trial confirm that FLAI‐GO is an active and safe treatment strategy for CD33‐positive AML patients aged ≤ 65 years, allowing a high ORR, a good disease debulking, favorable safety profile, low DDI, and subsequent high SCT rate. The encouraging results of this trial, consolidated by a long follow‐up, support the reintroduction of GO in clinical practice.
Multi‐gene panel testing improves diagnosis and management of patients with hereditary anemias
Authors: Russo Roberta , Andolfo Immacolata , Manna Francesco , Gambale Antonella , Marra Roberta , Rosato Barbara Eleni , Caforio Paola , Pinto Valeria , Pignataro Piero , Radhakrishnan Kottayam , Unal Sule , Tomaiuolo Giovanna , Forni Gian Luca , Iolascon Achille 
Published: February 2018

AbstractMutations in more than 70 genes cause hereditary anemias (HA), a highly heterogeneous group of rare/low frequency disorders in which we included: hyporegenerative anemias, as congenital dyserythropoietic anemia (CDA) and Diamond‐Blackfan anemia; hemolytic anemias due to erythrocyte membrane defects, as hereditary spherocytosis and stomatocytosis; hemolytic anemias due to enzymatic defects. The study describes the diagnostic workflow for HA, based on the development of two consecutive versions of a targeted‐NGS panel, including 34 and 71 genes, respectively. Seventy‐four probands from 62 unrelated families were investigated. Our study includes the most comprehensive gene set for these anemias and the largest cohort of patients described so far. We obtained an overall diagnostic yield of 64.9%. Despite 54.2% of cases showed conclusive diagnosis fitting well to the clinical suspicion, the multi‐gene analysis modified the original clinical diagnosis in 45.8% of patients (nonmatched phenotype‐genotype). Of note, 81.8% of nonmatched patients were clinically suspected to suffer from CDA. Particularly, 45.5% of the probands originally classified as CDA exhibited a conclusive diagnosis of chronic anemia due to enzymatic defects, mainly due to mutations in PKLR gene. Interestingly, we also identified a syndromic CDA patient with mild anemia and epilepsy, showing a homozygous mutation in CAD gene, recently associated to early infantile epileptic encephalopathy‐50 and CDA‐like anemia. Finally, we described a patient showing marked iron overload due to the coinheritance of PIEZO1 and SEC23B mutations, demonstrating that the multi‐gene approach is valuable not only for achieving a correct and definitive diagnosis, but also for guiding treatment.
Direct and Regioselective Amination of β‐Unsubstituted 5,15‐Diazaporphyrins with Amines: A Convenient Route to Near‐Infrared‐Responsive Diazaporphyrin Sensitizers
Authors: Omomo Satoshi , Sugai Takuma , Minoura Mao , Nakano Haruyuki , Matano Yoshihiro 
Published: February 2018

AbstractWe have established a convenient method for the base‐promoted direct amination of β‐unsubstituted 5,15‐diazaporphyrins (DAPs) with secondary and primary amines to produce 3,7,13,17‐tetraamino‐ and 3‐amino‐DAPs, respectively, regioselectively. The amino groups attached at the periphery cause significant red shifts of the absorption bands as a result of their perturbation of the HOMO and/or LUMO in the DAP π‐system. The palladium complex of a 3,7,13,17‐tetrakis(diphenylamino)‐DAP generated singlet oxygen in high yield under irradiation with near‐infrared light.
Practical tool for sampling and fast analysis of large cocaine seizures
Authors: Eliaerts J. , Meert N. , Van Durme F. , Samyn N. , De Wael K. , Dardenne P. 
Published: February 2018

AbstractLarge quantities of illicit drugs are frequently seized by law enforcement. In such cases, a representative number of samples needs to be quickly examined prior to destruction. No procedure has yet been set up which rapidly provides information regarding the homogeneity of the samples, the presence of controlled substances, and the degree of purity. This study establishes a protocol for fast analysis of cocaine and its most common cutting agent, levamisole, in large seizures. The protocol is based on a hypergeometric sampling approach combined with Fourier‐transform infrared (FTIR) spectrometry and support vector machines (SVM) algorithms as analysis methods. To demonstrate the practical use of this approach, 5 large cocaine seizures (consisting between 45 and 85 units) were analysed simultaneously with gas chromatography–mass spectrometry (GC–MS), gas chromatography–flame ionisation detector (GC–FID), and a portable FTIR spectrometer using attenuated total reflectance (ATR) sampling combined with SVM models. According to the hypergeometric sampling plan of the guidelines of the Drugs Working Group (DWG) of the European Network of Forensic Science Institutes (ENFSI), the required number of subsamples ranged between 19 and 23. Considering the identification analyses, the SVM models detected cocaine and levamisole in all subsamples of Cases 1 to 5 (100% correct classification), which was confirmed by GC–MS analysis. Considering the quantification analyses, the SVM models were able to estimate the cocaine and levamisole content in each subsample, compared to GC–FID data. The developed strategy is easy, cost effective, and provides immediate information about both the presence and concentration of cocaine and levamisole. By using this new strategy, the number of confirmation analyses with laborious and expensive chromatographic techniques could be significantly reduced.
One‐Pot Catalytic Copolymerization of Unsaturated Plant Oils or Fatty Acid Methyl Esters with Ethylene
Authors: Fleckhaus André , Fokou Patrice A. , Klaassen Gerd , Rüsch gen. Klaas Mark 
Published: February 2018

Until now natural fatty acid esters with their internal CC‐bonds cannot be used as substrates for catalytic polymerization reactions. It is always necessary to convert them to terminal unsaturated fatty acid esters first. Therefore, we now developed a two‐catalyst system consisting of an isomerization catalyst and a Brookhart polymerization catalyst. With this system of isomerizing copolymerization (IsoCoP) it is possible to copolymerize high oleic plant oils or fatty acid methyl esters with ethylene in a one‐pot reaction. The resulting polymers have molecular weights above 30 kDa and can contain more than 20 weight percent plant oils. The copolymers of fatty acid methyl esters are soluble in organic solvents. The predominant part of the copolymers from plant oils is usually soluble. The insoluble fraction is soluble after hydrolysis indicating a rare cross‐linking of polyolefin chains by triglycerol units. The catalytic system is effective yielding polymers with a productivity of up to 100 kg polymer per mole catalyst and pretty robust. Plants oils need to be purified only by a simple filtration over alumina before copolymerization.Practical Application: The possibility to copolymerize plant oils with ethylene leads to a new class of polymers and may enhance the usage of plant oils for plastics greatly. The new polymers might be used, e.g., for tackifiers or primers.High oleic plant oils as well as unsaturated fatty acid methyl esters can be directly copolymerized with ethylene by a combination of an isomerization catalyst with a Brookhart polymerization catalyst.
Quantifying Energy Systems Resilience—A Simulation Approach to Assess Recovery
Authors: Kyriakidis Miltos , Lustenberger Peter , Burgherr Peter , Dang Vinh N. , Hirschberg Stefan 
Published: February 2018

AbstractResilience often addresses preparedness of systems and social units to internal or/and external hazards, and the subsequent recovery. Preparedness is primarily associated with the designed, as‐built, pre‐disaster phase of a system, whereas recovery deals with the post‐disruption response. As the damage of a system and the service disruption are associated with inherent properties of its components, in addition to the magnitude of the hazard, recovery is additionally influenced by external factors, such as the state of the access infrastructure. This study demonstrates a simulation approach to quantify a metric of resilience, in this case the integral loss of function or service. A case study on the impact of floods on a natural gas network is presented, considering the geospatial location of the network, the areas with and without service, the required times for travel to the damage locations, and inspection/repair. The results underscore how the priorities selected for recovery can lead to strikingly different outcomes in terms of the resilience measure.
Surface sulfonamide modification of poly(N‐isopropylacrylamide)‐based block copolymer micelles to alter pH and temperature responsive properties for controlled intracellular uptake
Authors: Cyphert Erika L. , von Recum Horst A. , Yamato Masayuki , Nakayama Masamichi 
Published: February 2018

AbstractTwo different surface sulfonamide‐functionalized poly(N‐isopropylacrylamide)‐based polymeric micelles were designed as pH‐/temperature‐responsive vehicles. Both sulfadimethoxine‐ and sulfamethazine‐surface functionalized micelles were characterized to determine physicochemical properties, hydrodynamic diameters, zeta potentials, temperature‐dependent size changes, and lower critical solution temperatures (LCST) in both pH 7.4 and 6.8 solutions (simulating both physiological and mild low pH conditions), and tested in the incorporation of a proof‐of‐concept hydrophobic antiproliferative drug, paclitaxel. Cellular uptake studies were conducted using bovine carotid endothelial cells and fluorescently labeled micelles to evaluate if there was enhanced cellular uptake of the micelles in a low pH environment. Both variations of micelles showed enhanced intracellular uptake under mildly acidic (pH 6.8) conditions at temperatures slightly above their LCST and minimal uptake at physiological (pH 7.4) conditions. Due to the less negative zeta potential of the sulfamethazine‐surface micelles compared to sulfadimethoxine‐surface micelles, and the proximity of their LCST to physiological temperature (37°C), the sulfamethazine variation was deemed more amenable for clinically relevant temperature and pH‐stimulated applications. Nevertheless, we believe both polymeric micelle variations have the capacity to be implemented as an intracellular drug or gene delivery system in response to mildly acidic conditions. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1552–1560, 2018.
Interference of in vitro hemolysis complete blood count
Authors: de Jonge Gabriela , dos Santos Talita L. , Cruz Bruno R. , Simionatto Mackelly , Bittencourt Jeanine I. M. , Krum Everson A. , Moss Mariane F. , Borato Danielle Cristyane K. 
Published: February 2018

BackgroundHemolysis may occur in vivo, under pathological conditions, or in vitro, related to pre‐analytical errors. Hemolyzed samples may produce unreliable results, leading to errors in diagnostic and monitoring evaluations. This study aims to evaluate the interference of in vitro hemolysis on the interpretation of the parameters of the blood cell‐counting performed by the impedance method.MethodsPeripheral blood samples were collected in anticoagulant K2‐EDTA and subsequently divided into three 1.0 mL aliquots. The first aliquot was not subjected to any intervention, and the second and third aliquots were passed 5 and 10 times through a small‐gauge needle to produce scalar amounts of hemolysis. Hematological tests were performed by Hemacounter 60‐RT 7600®.ResultsComparison of the samples with different degrees of hemolysis showed a decrease in red blood cells count and hematocrit counts and increase in mean corpuscular hemoglobin concentration and platelet count in samples with a high degree of hemolysis. According to the accepted clinical point of view, the samples with a high degree of hemolysis exceeded the desirable bias, presenting decrease in red blood cells count, hematocrit and mean corpuscular volume, and increase in red cell distribution width, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelet counts. However, samples with a mild degree of hemolysis showed only a slight increase in mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelet count.ConclusionThis study demonstrated that in vitro hemolysis can decrease the clinical and analytical reliability of the assessment of the blood count.
Simultaneous determination and qualitative analysis of six types of components in Naoxintong capsule by miniaturized matrix solid‐phase dispersion extraction coupled with ultra high‐performance liquid chromatography with photodiode array detection and quadrupole time‐of‐flight mass spectrometry
Authors: Wang Huilin , Jiang Yan , Ding Mingya , Li Jin , Hao Jia , He Jun , Wang Hui , Gao Xiu‐Mei , Chang Yan‐Xu 
Published: February 2018

AbstractA simple and effective sample preparation process based on miniaturized matrix solid‐phase dispersion was developed for simultaneous determination of phenolic acids (gallic acid, chlorogenic acid, ferulic acid, 3,5‐dicaffeoylqunic acid, 1,5‐dicaffeoylqunic acid, rosmarinic acid, lithospermic acid, and salvianolic acid B), flavonoids (kaempferol‐3‐O‐rutinoside, calycosin, and formononetin), lactones (ligustilide and butyllidephthalide), monoterpenoids (paeoniflorin), phenanthraquinones (cryptotanshinone), and furans (5‐hydroxymethylfurfural) in Naoxintong capsule by ultra high‐performance liquid chromatography. The optimized condition was that 25 mg Naoxintong powder was blended homogeneously with 100 mg Florisil PR for 4 min. One milliliter of methanol/water (75:25, v/v) acidified by 0.05% formic acid was selected to elute all components. It was found that the recoveries of the six types of components ranged from 61.36 to 96.94%. The proposed miniaturized matrix solid‐phase dispersion coupled with ultra high‐performance liquid chromatography was successfully applied to simultaneous determination of the six types of components in Naoxintong capsules. The results demonstrated that the proposed miniaturized matrix solid‐phase dispersion coupled with ultra high‐performance liquid chromatography could be used as an environmentally friendly tool for the extraction and determination of multiple bioactive components in natural products.
Mice Fed a High‐Cholesterol Diet Supplemented with Quercetin‐3‐Glucoside Show Attenuated Hyperlipidemia and Hyperinsulinemia Associated with Differential Regulation of PCSK9 and LDLR in their Liver and Pancreas
Authors: Mbikay Majambu , Mayne Janice , Sirois Francine , Fedoryak Olesya , Raymond Angela , Noad Jennifer , Chrétien Michel 
Published: February 2018

ScopeHepatic LDL receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9) regulate the clearance of plasma LDL‐cholesterol (LDL‐C): LDLR promotes it, and PCSK9 opposes it. These proteins also express in pancreatic β cells. Using cultured hepatocytes, we previously showed that the plant flavonoid quercetin‐3‐glucoside (Q3G) inhibits PCSK9 secretion, stimulated LDLR expression, and enhanced LDL‐C uptake. Here, we examine whether Q3G supplementation could reverse the hyperlipidemia and hyperinsulinemia of mice fed a high‐cholesterol diet, and how it affects hepatic and pancreatic LDLR and PCSK9 expression.Methods and resultsFor 12 weeks, mice are fed a low‐ (0%) or high‐ (1%) cholesterol diet (LCD or HCD), supplemented or not with Q3G at 0.05 or 0.1% (w/w). Tissue LDLR and PCSK9 is analyzed by immunoblotting, plasma PCSK9 and insulin by ELISA, and plasma cholesterol and glucose by colorimetry. In LCD‐fed mice, Q3G has no effect. In HCD‐fed mice, it attenuates the increase in plasma cholesterol and insulin, accentuates the decrease in plasma PCSK9, and increases hepatic and pancreatic LDLR and PCSK9. In cultured pancreatic β cells, however, it stimulates PCSK9 secretion.ConclusionIn mice, dietary Q3G could counter HCD‐induced hyperlipidemia and hyperinsulinemia, in part by oppositely modulating hepatic and pancreatic PCSK9 secretion.
Use of Lactobacillus for Improved Growth and Enhanced Biochemical, Hematological, and Digestive Enzyme Activity in Common Carp at Mazandaran, Iran
Authors: Valiallahi Jalal , Pourabasali Mohsen , Janalizadeh Elham , Bucio Adolfo 
Published: February 2018

Abstract
The aim of this study was to investigate the effects of Lactobacillus plantarum strain 44A doses on growth performance, feed utilization, blood serum parameters, and digestive enzyme activities in Common Carp Cyprinus carpio fingerlings. This bacterium was isolated from healthy Striped Mullet Mugil cephalus. Lactobacillus plantarum was added to the diet in three levels (1.5 × 106, 3 × 106, and 4.5 × 106 colony-forming units [CFU]/mg of feed). A control diet was used as a reference. The results indicated that feed conversion ratio (FCR), specific growth rate, daily growth coefficient, protein efficiency ratio, condition factor, thermal-unit growth coefficient, and survival rate of Common Carp fingerlings were best for the treatment group fed with L. plantarum at the 4.5 × 106 CFU/mg level. In general, all L. plantarum treatments had better hematological parameters relative to the control, including red blood cell count, total leucocytes, hematocrit, hemoglobin, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration. A significant increase in the total protein and globulin concentration in blood serum was found in all probiotic-treated fishes compared with the control group, whereas no significant difference was observed in albumin levels. Other observed changes included lower cortisol and glucose contents in serum blood from probiotic-treated fingerlings than in the control group. Serum lysozyme activity was also considerably greater in fish fed a higher level of L. plantarum compared with the control fish. Mean protease and amylase activities of all probiotic treatment groups were significantly higher than those in the control fish. There was no significant difference in lipase level. In conclusion, the improved enzyme activities obtained with the L. plantarum diets suggest that the addition of probiotics improved diet digestibility, including protein, starch, and fattiness, and resulted in better growth performance and feed efficiency (FCR) in Common Carp.