bluuebunny/crossref_metadata_2025_split huggingface dataset:
CBP and P300 regulate distinct gene networks required for human primary myoblast differentiation and muscle integrity
Authors: Fauquier Lucas , Azzag Karim , Parra Marco Antonio Mendoza , Quillien Aurélie , Boulet Manon , Diouf Sarah , Carnac Gilles , Waltzer Lucas , Gronemeyer Hinrich , Vandel Laurence 
Published: August 2018

AbstractThe acetyltransferases CBP and P300 have been implicated in myogenesis in mouse immortalized cell lines but these studies focused only on the expression of a handful of myogenic factors. Hence, the respective role of these two related cofactors and their impact at global scale on gene expression rewiring during primary myoblast differentiation remain unknown. Here, we characterised the gene networks regulated by these two epigenetic enzymes during human primary myoblast differentiation (HPM). We found that CBP and p300 play a critical role in the activation of the myogenic program and mostly regulate distinct gene sets to control several aspects of HPM biology, even though they also exhibit some degree of redundancy. Moreover, CBP or P300 knockdown strongly impaired muscle cell adhesion and resulted in the activation of inflammation markers, two hallmarks of dystrophic disease. This was further validated in zebrafish where inhibition of CBP and P300 enzymatic activities led to cell adhesion defects and muscle fiber detachment. Our data highlight an unforeseen link between CBP/P300 activity and the emergence of dystrophic phenotypes. They thereby identify CBP and P300 as mediators of adult muscle integrity and suggest a new lead for intervention in muscular dystrophy.
Alterations of 63 hub genes during lingual carcinogenesis in C57BL/6J mice
Authors: Liu Hua , Li Jianjiao , Yang Ying , Liu Liu , Yu Lifu , Tu Minsong , Yuan Ruihong , Yue Wanyuan , Luo Qi , Ruan Yonghua , Dai Xiaoming 
Published: August 2018

AbstractTo identify potential biomarkers of lingual cancer, 75 female C57BL/6J mice were subjected to 16-week oral delivery of 4-nitroquinoline-1-oxide (4NQO; 50 mg/L), with 10 mice used as controls. Lingual mucosa samples representative of normal tissue (week 0) and early (week 12) and advanced (week 28) tumorigenesis were harvested for microarray and methylated DNA immunoprecipitation sequencing (MeDIP-Seq). Combined analysis with Short Time-series Expression Miner (STEM), the Cytoscape plugin cytoHubba, and screening of differentially expressed genes enabled identification of 63 hub genes predominantly altered in the early stage rather than the advanced stage. Validation of microarray results was carried out using qRT-PCR. Of 63 human orthologous genes, 35 correlated with human oral squamous cell carcinoma. KEGG analysis showed “pathways in cancer”, involving 13 hub genes, as the leading KEGG term. Significant alterations in promoter methylation were confirmed at Tbp, Smad1, Smad4, Pdpk1, Camk2, Atxn3, and Cdh2. HDAC2, TBP, and EP300 scored ≥10 on Maximal Clique Centrality (MCC) in STEM profile 11 and were overexpressed in human tongue cancer samples. However, expression did not correlate with smoking status, tumor differentiation, or overall survival. These results highlight potentially useful candidate biomarkers for lingual cancer prevention, diagnosis, and treatment.
Inhibition of NOS- like activity in maize alters the expression of genes involved in H2O2 scavenging and glycine betaine biosynthesis
Authors: Phillips Kyle , Majola Anelisa , Gokul Arun , Keyster Marshall , Ludidi Ndiko , Egbichi Ifeanyi 
Published: August 2018

AbstractNitric oxide synthase-like activity contributes to the production of nitric oxide in plants, which controls plant responses to stress. This study investigates if changes in ascorbate peroxidase enzymatic activity and glycine betaine content in response to inhibition of nitric oxide synthase-like activity are associated with transcriptional regulation by analyzing transcript levels of genes (betaine aldehyde dehydrogenase) involved in glycine betaine biosynthesis and those encoding antioxidant enzymes (ascorbate peroxidase and catalase) in leaves of maize seedlings treated with an inhibitor of nitric oxide synthase-like activity. In seedlings treated with a nitric oxide synthase inhibitor, transcript levels of betaine aldehyde dehydrogenase were decreased. In plants treated with the nitric oxide synthase inhibitor, the transcript levels of ascorbate peroxidase-encoding genes were down-regulated. We thus conclude that inhibition of nitric oxide synthase-like activity suppresses the expression of ascorbate peroxidase and betaine aldehyde dehydrogenase genes in maize leaves. Furthermore, catalase activity was suppressed in leaves of plants treated with nitric oxide synthase inhibitor; and this corresponded with the suppression of the expression of catalase genes. We further conclude that inhibition of nitric oxide synthase-like activity, which suppresses ascorbate peroxidase and catalase enzymatic activities, results in increased H2O2 content.
0.45‐V 5.4‐nW switched‐capacitor bandgap reference with intermittent operation and improved supply immunity
Authors: Luo Ziyang , Lu Yan , Martins Rui P. 
Published: August 2018

Switched‐capacitor networks have been used as bandgap references (BGRs) to replace the area‐consuming resistors in ultra‐low power applications, incorporated with voltage doubler (2X) charge pumps to operate at low supply voltages. However, the open‐loop charge pumps are sensitive to supply and load variations. Therefore, in this work, constant bias currents are added to the cross‐coupled 2X charge pumps to reduce the supply sensitivity, and also to reduce the output ripples without using large capacitors. To further reduce the power consumption, a low‐leakage sample and hold (S&H) circuit is used for an intermittent operation, the active/idle duty ratio of the BGR core is 1/15. Designed in standard 65 nm CMOS, the proposed BGR reaches a temperature coefficient of 64 ppm/°C consuming only 5.4 nW with a minimum supply of 0.45 V (simulated).
Low RMS phase error 28 GHz 5‐bit switch type phase shifter for 5G applications
Authors: Tsai J.‐H. , Lin F.‐M. , Xiao H. 
Published: August 2018

A 28 GHz 5‐bit switch‐type phase shifter (STPS) using 0.18 μm CMOS technology is presented for 5G mobile communication applications. Utilising a process variation insensitive transmission line‐based bridged T‐type topology for 11.25°, 22.5°, 45°, 90° 1‐bit phase shifters and high‐pass/low‐pass network switched‐path‐type topology for 180° 1‐bit phase shifter, the 5‐bit STPS achieves ultra‐low root mean square (RMS) phase error of 1.7° and low RMS amplitude error of 0.59 dB at 28 GHz with intrinsic digital phase‐control mechanism. The measured insertion loss of all 32 states is 15 ± 1.5 dB at 28 GHz with zero dc consumption.
Mercurius solubilis Hahnemanni bei Kälberdurchfall
Authors: Hentschel Brigitte , Hornig Bernhard 
Published: August 2018

ZusammenfassungDurchfallerkrankungen sind nach wie vor die häufigste Todesursache neugeborener Kälber. Trotz Optimierung der äußeren Umstände, kommt es unter Praxisbedingungen noch immer zu massiven Ausbrüchen mit hohen Tierverlusten. Hier stößt die Schulmedizin nur allzu oft an ihre Grenzen. Obwohl sehr viele homöopathische Arzneien bei Kälberdurchfall infrage kommen, sticht Mercurius solubilis Hahnemanni aus der Masse hervor. Durch die Darstellung offensichtlicher Parallelen zwischen dem Arzneimittelbild von Mercurius und der Pathophysiologie von Kälberdurchfall, wird sein Nutzen bei dieser Krankheit deutlich gemacht und mittels zwei anschaulichen Fallbeispielen aus der Praxis belegt.
„Da kann man sich ja totklingeln, geht ja keiner ran“ – Schnittstellenprobleme zwischen stationärer, hausärztlicher und ambulant-fachspezialisierter Patientenversorgung aus Sicht Dresdner Hausärzte
Authors: Lang Caroline , Gottschall Mandy , Sauer Maik , Köberlein-Neu Juliane , Bergmann Antje , Voigt Karen 
Published: August 2018

Zusammenfassung
Ziel der Studie Ziel der Studie war es, Schnittstellenprobleme zwischen stationärer, hausärztlicher und ambulant-fachspezialisierter Patientenversorgung bei älteren multimorbiden Patienten aus hausärztlicher Sicht aufzudecken.
Methodik Die Datenerhebung erfolgte im Rahmen der Pilotstudie „Multimedikation und ihre Folgen für die hausärztliche Patientenversorgung in Sachsen“ und umfasste leitfadengestützte Interviews mit 7 Hausärzten. Die Interviews wurden vollständig transkribiert und inhaltlich in Anlehnung an die induktive Kategorienentwicklung nach Mayring analysiert.
Ergebnisse An stationärer zu ambulant-hausärztlichen Schnittstelle zeigten sich v. a. Probleme bei der intersektoralen Kommunikation und Kooperation, dem Entlassmanagement sowie beim Medikationsmanagement. An der Schnittstelle vom Hausarzt zu ambulant-fachspezialisierten Kollegen zeigten sich Probleme hinsichtlich der freien Arztwahl, des Terminmanagements, des ärztlichen Entscheidungsfindungsprozesses, des Medikationsmanagements sowie bei der Nutzung der elektronischen Gesundheitskarte. Schnittstellenunabhängig wurden die mangelnde Kommunikation und Kooperation als zentrale Herausforderungen herausgearbeitet.
Schlussfolgerung Aus hausärztlicher Sicht gibt es eine Vielzahl von Problemen zu allen angrenzenden Schnittstellen. Auffällig hierbei ist die mangelnde Kommunikation und Kooperation zwischen den verschiedenen Beteiligten. Als mögliche Ursachen zeigen sich die häufig sehr unterschiedlichen Arbeitsweisen und Rahmenbedingungen, personellen und materiellen Ressourcen sowie Therapieintentionen und -ansätze zwischen den settingspezifischen Schnittstellen. Ein möglicher Ansatz zur Verbesserung der Situation kann das seit 2016 bestehende Gesetz für sichere digitale Kommunikation und Anwendungen im Gesundheitswesen sein. Es verspricht u. a. einen sicheren und zügigen inter- und intrasektoralen Informationsaustausch von Patientendaten. Übergeordnetes Ziel der Gesundheitsversorgung in Deutschland sollte ein optimiertes und prozessorientiertes Schnittstellenmanagement sein, um Patienten einen sicheren und nahtlosen sektoralen Übergang zu gewährleisten.
Synthesis of Functionalized 5-Amino-3(2H)-furanones via Base-Catalyzed Ring-Cleavage/Recyclization of 4-Cyano-3(2H)-furanones in the Presence of Water
Authors: Shemyakina Olesya , Volostnykh Olga , Stepanov Anton , Ushakov Igor , Borodina Tatyana 
Published: August 2018

5-Alkyl/aryl/hetaryl-4-cyano-3(2H)-furanones undergo ring-cleavage/recyclization in the presence of water under mild conditions [MOH (M = Na, K), aqueous ethanol, 20–25 °C] to afford 4-acyl/aroyl/hetaroyl-5-amino-3(2Н)-furanones in 75–99% yields.
Interrupted Ugi and Passerini Reactions: An Underexplored Treasure Island
Authors: Giustiniano Mariateresa , Moni Lisa , Tron Gian , Sangaletti Luca , Pelliccia Sveva , Basso Andrea , Novellino Ettore 
Published: August 2018

The formation of the key short-lived intermediate nitrilium ion in the Passerini and Ugi reactions was recognized early in the discovery of these two-multicomponent transformations. Surprisingly, the idea of intramolecularly intercepting it, eluding the attack by the carboxylate, and thus interrupting the normal course of the reaction, was missed by chemists for several decades. In this review we describe, in an exhaustive manner, the reported synthetic approaches, which can be categorized as interrupted Passerini/Ugi reactions. Besides, the clear goal of this review is to show the potential of these transformations, whilst highlighting the underexplored combinations of reagents toward the identification of novel interrupted Passerini/Ugi reactions.1	Introduction2	The Interrupted Ugi Reaction2.1	The Interrupting Functional Group is Present on the Amine Reactant2.2	The Interrupting Functional Group is Present on the Isocyanide	Reactant2.3	The Interrupting Functional Group is Present on the Carbonyl	Reactant3	The Interrupted Passerini Reaction3.1	The Interrupting Functional Group is Present on the Carbonyl	Reactant3.2	The Interrupting Functional Group is Present on the Isocyanide	Reactant4	Conclusions and Future Outlook
Recent Advances in the Synthesis of Hetero- and Carbocyclic Compounds­ and Complexes Based on Acenaphthylene-1,2-dione
Authors: Yavari Issa , Khajeh-Khezri Aliyeh 
Published: August 2018

Acenaphthylene-1,2-dione has been utilized in a wide range of reactions as a starting material for the synthesis of hetero- and carbocyclic compounds and complexes. This review provides a short summary of the recent advances in the application of acenaphthylene-1,2-dione in the synthesis of hetero- and carbocyclic systems and bioactive compounds. In addition, the applications of acenaphthylene-1,2-dione in the synthesis of spiro compounds, propellanes, and ligands in catalyst reactions, from 2002 to early 2018, are included.1	Introduction2	Synthesis of Spiro Compounds Employing Acenaphthylene-1,2-dione2.1	Methods for the Construction of Spiro Compounds2.1.1	By 1,3-Dipolar Cycloaddition of Acenaphthylene-1,2-dione via	Azomethine Ylides2.1.2	By Multicomponent Reactions of Acenaphthylene-1,2-dione with	C–H Acidic Compounds2.1.3	By Reaction of Acenaphthylene-1,2-dione with Zwitterionic	Intermediates2.1.4	By Substitution and Multicomponent Reactions of Acenaphth-	ylene-1,2-dione with Different Nucleophiles3	Synthesis of Propellanes by Employing Acenaphthylene-1,2-dione3.1	Methods for the Construction of Propellanes Based on Acenaph-	thylene-1,2-dione3.1.1	By Reaction of Acenaphthylene-1,2-dione with Nucleophiles3.1.2	By Reaction of Acenaphthylene-1,2-dione with Binucleophiles4	Synthesis of Ligands Employing Acenaphthylene-1,2-dione for	Catalyst Reactions5	Synthesis of Novel Hetero- and Carbocyclic Compounds Employing	Acenaphthylene-1,2-dione5.1	By Reaction of Acenaphthylene-1,2-dione with Nucleophiles5.2	By Reaction of Acenaphthylene-1,2-dione with Zwitterionic	Intermediates5.3	By Ring Opening and Ring Enlargement6	Conclusion
HTRA2 Defect: A Recognizable Inborn Error of Metabolism with 3-Methylglutaconic Aciduria as Discriminating Feature Characterized by Neonatal Movement Disorder and Epilepsy—Report of 11 Patients
Authors: Morin Gilles , Nouri Maria , Brandau Oliver , Saadi Nebal , Nouri Mohammed , van den Broek Florence , Prokisch Holger , Mayr Johannes , Wortmann Saskia , Kovacs-Nagy Reka 
Published: August 2018

AbstractNeonatal-onset movement disorders, especially in combination with seizures, are rare and often related to mitochondrial disorders. 3-methylglutaconic aciduria (3-MGA-uria) is a marker for mitochondrial dysfunction. In particular, consistently elevated urinary excretion of 3-methylglutaconic acid is the hallmark of a small but growing group of inborn errors of metabolism (IEM) due to defective phospholipid remodeling or mitochondrial membrane-associated disorders (mutations in TAZ, SERAC1, OPA3, CLPB, DNAJC19, TMEM70, TIMM50). Exome/genome sequencing is a powerful tool for the diagnosis of the clinically and genetically heterogeneous mitochondrial disorders. Here, we report 11 individuals, of whom 2 are previously unpublished, with biallelic variants in high temperature requirement protein A2 (HTRA2) encoding a mitochondria-localized serine protease. All individuals presented a recognizable phenotype with neonatal- or infantile-onset neurodegeneration and death within the first month of life. Hallmark features were central hypopnea/apnea leading to respiratory insufficiency, seizures, neutropenia, 3-MGA-uria, tonus dysregulation, and dysphagia. Tremor, jitteriness, dystonia, and/or clonus were also common. HTRA2 defect should be grouped under the IEM with 3-MGA-uria as discriminating feature. Clinical characteristics overlap with other disorders of this group suggesting a common underlying pathomechanism. Urinary organic acid analysis is a noninvasive and inexpensive test that can guide further genetic testing in children with suggestive clinical findings.
Chronic Mesenteric Ischemia: Clinical Diagnosis, Imaging Characteristics, and Endovascular Management
Authors: Dalag Leonard , Lorenz Jonathan , Tullius Thomas 
Published: August 2018

AbstractChronic mesenteric ischemia (CMI) is a complex disorder, which typically affects elderly patients who classically present with longstanding abdominal pain and nonspecific clinical symptoms, such as food aversion and weight loss. The disease progression is often gradual due to a rich collateral network, and symptoms are generally not clinically apparent until two or more primary visceral arteries are affected. Symptomatic stenosis or occlusion of the mesenteric arteries can be the result of multiple etiologies, although atherosclerosis is the most common. Given its low incidence, a high clinical suspicion is necessary for diagnosis and to prevent a delay in treatment. Multiple imaging modalities are utilized to confirm the presence of mesenteric vessel involvement and to guide treatment. While open surgical revascularization was once the definitive treatment, endovascular management has become the first-line treatment for atherosclerotic CMI. The endovascular approach with stent placement has shown excellent primary success and encouraging long-term outcomes with reduction in morbidity and mortality compared with open surgical revascularization. Although controversial, median arcuate ligament syndrome (MALS) is a less common cause of CMI, which can affect a more varied patient population. Clinical approach, imaging characteristics, and treatment for this syndrome will also be discussed.
A Systematic Review of the Technology Acceptance Model in Health Informatics
Authors: Rahimi Bahlol , Nadri Hamed , Lotfnezhad Afshar Hadi , Timpka Toomas 
Published: August 2018

Background One common model utilized to understand clinical staff and patients' technology adoption is the technology acceptance model (TAM).
Objective This article reviews published research on TAM use in health information systems development and implementation with regard to application areas and model extensions after its initial introduction.
Method An electronic literature search supplemented by citation searching was conducted on February 2017 of the Web of Science, PubMed, and Scopus databases, yielding a total of 492 references. Upon eliminating duplicates and applying inclusion and exclusion criteria, 134 articles were retained. These articles were appraised and divided into three categories according to research topic: studies using the original TAM, studies using an extended TAM, and acceptance model comparisons including the TAM.
Results The review identified three main information and communication technology (ICT) application areas for the TAM in health services: telemedicine, electronic health records, and mobile applications. The original TAM was found to have been extended to fit dynamic health service environments by integration of components from theoretical frameworks such as the theory of planned behavior and unified theory of acceptance and use of technology, as well as by adding variables in specific contextual settings. These variables frequently reflected the concepts subjective norm and self-efficacy, but also compatibility, experience, training, anxiety, habit, and facilitators were considered.
Conclusion Telemedicine applications were between 1999 and 2017, the ICT application area most frequently studied using the TAM, implying that acceptance of this technology was a major challenge when exploiting ICT to develop health service organizations during this period. A majority of the reviewed articles reported extensions of the original TAM, suggesting that no optimal TAM version for use in health services has been established. Although the review results indicate a continuous progress, there are still areas that can be expanded and improved to increase the predictive performance of the TAM.
Does the Robotic Arm and Preoperative CT Planning Help with 3D Intraoperative Total Knee Arthroplasty Planning?
Authors: Marchand Robert C. , Sodhi Nipun , Bhowmik-Stoker Manoshi , Scholl Laura , Condrey Caitlin , Khlopas Anton , Sultan Assem A. , Newman Jared M. , Mont Michael A. 
Published: August 2018

Although several studies highlight the advantages of robotic arm-assisted total knee arthroplasty (RA-TKA), few investigate its intraoperative outcome. Therefore, the purpose of this study was to analyze the RA-TKA's ability to assist with intraoperative correction of: (1) flexion and (2) extension gaps, as well as its ability to (3) accurately predict implant sizes. Additionally, in this RA-TKA cohort, length of stay, complications, and readmissions were assessed. A total of 335 patients who underwent RA-TKA were included. The robotic software virtually measured the intraoperative prebone cut extension and flexion gaps. Differences in medial versus lateral prebone cut extension and flexion gaps were calculated. A total of 155 patients (46%) had an extension gap difference of between –2 and 2 mm (mean, –0.3 mm), while 119 patients (36%) had a flexion gap difference of between –2 and 2 mm (mean, –0.6 mm). Postbone cut differences in medial versus lateral flexion and extension gaps were measured. Balanced knees were considered to have a medial and lateral flexion gap difference within 2 mm. The robot-predicted implant size was also compared with the final implant size. Additionally, lengths of stay, complications, and readmissions were assessed. All patients achieved a postbone cut extension gap difference between –1 and 1 mm (mean, –0.1 mm). A total of 332 patients (99%) achieved a postbone cut flexion gap difference of between –2 and 2 mm (mean, 0 mm). For 98% of prostheses, the robotic software predicted within 1 implant size the actual tibial or femoral implant size used.The mean length of stay was found to be 2 days. No patients suffered from superficial skin infection, pin site infections or fractures, soft tissue damage, and no robotic cases were converted to manual TKA due to intraoperative complications. A total of 8 patients (2.2%) were readmitted; however, none were directly related to robotic use. The robotic software and use of a preoperative computed tomography (CT) substantially helped with intraoperative planning and accurate prediction of implant sizes. Therefore, based on the results of this study, the RA-TKA device does, in fact, provide considerable intraoperative assistance.
Anticoagulant Effects of Dabigatran in Paediatric Patients Compared with Adults: Combined Data from Three Paediatric Clinical Trials
Authors: Maas Hugo , Gropper Savion , Huang Fenglei , Stangier Joachim , Tartakovsky Igor , Brueckmann Martina , Halton Jacqueline , Mitchell Lesley 
Published: August 2018

Background Physiological age-related changes in the haemostatic and coagulation systems result in differing anticoagulant assay responses to standard anticoagulants. Therefore, we investigated the response of anticoagulant assays to dabigatran etexilate (DE) in children compared with adults.
Objective This article assesses the relationship between plasma dabigatran concentration and coagulation assay results across age groups in children and adults.
Patients and Methods Data from three clinical trials in which children received DE following standard of care for venous thromboembolism were compared with data from adult clinical trials. The effects of dabigatran concentration on diluted thrombin time (dTT), ecarin clotting time (ECT) and activated partial thromboplastin time (aPTT) were analysed graphically and with modelling.
Results The concentration–dTT relationships were consistent in children across all ages and adults in the graphical analysis. For ECT and aPTT, relationships based on ratios over baseline were similar across all ages; absolute clotting times showed that the same exposure resulted in longer clotting times in some of the children aged < 1 year versus adults. Modelling showed concentration–clotting time relationships for all three assays were largely comparable between adults and children, except in those aged < 2 months, in whom there was a slight upward shift in ECT and aPTT relative to adults.
Conclusion Results suggest that developmental haemostatic changes will have little impact on response to DE. However, further paediatric clinical trials assessing the relationship between coagulation assay responses and clinical outcomes will be needed to confirm this finding.