bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aluminum adsorption on graphene: Theoretical study of dispersion effects
Authors: Rossi Fernández Ana C. , Domancich Nicolás F. , Ferullo Ricardo M. , Castellani Norberto J. 
Published: May 2019

The interaction between a single atom and graphene is an example in which the density functional theory (DFT) presents serious difficulties in giving an appropriate description of the adsorbate–substrate interaction, giving also different predictions according to the chosen approximation. The present calculations sustain that the inclusion of dispersion interactions in the framework of DFT for the Al/graphene system lead to potential energy curves of different nature according to the theoretical approach employed. The adsorption of an Al atom on the graphene surface was studied using both cluster and slab models. Cluster DFT–PBE calculations show the presence of a minimum at hollow site at an Al–graphene distance of about 2.1–2.3 Å corresponding to an exothermic state. Conversely, under B3LYP the same adsorption mode is endothermic. In comparison, our MP2 reference calculations predict the formation of two minima, both of exothermic nature, separated by an important energy barrier (about 0.2–0.4[Formula: see text]eV). The incorporation of empirical van der Walls (vdW) corrections to B3LYP changes the original behavior, giving an exothermic adsorption; furthermore, it produces a second, more external minimum. Slab calculations with PBE, and specially using the vdW-DF2 functional, predict also the formation of a minimum of very low depth at about 3.1 Å. The analysis of results obtained with cluster and slab models sustains that the bonding of the inner minima is of ionic character while that of the external ones is of dispersion character.
LncRNA SNHG10 Facilitates Hepatocarcinogenesis and Metastasis by Modulating Its Homolog SCARNA13 via a Positive Feedback Loop
Authors: Lan Tian , Yuan Kefei , Yan Xiaokai , Xu Lin , Liao Haotian , Hao Xiangyong , Wang Jinju , Liu Hong , Chen Xiangzheng , Xie Kunlin , Li Jiaxin , Liao Mingheng , Huang Jiwei , Zeng Yong , Wu Hong 
Published: May 2019

AbstractUnderstanding the roles of noncoding RNAs (ncRNA) in tumorigenesis and metastasis would establish novel avenues to identify diagnostic and therapeutic targets. Here, we aimed to identify hepatocellular carcinoma (HCC)–specific ncRNA and to investigate their roles in hepatocarcinogenesis and metastasis. RNA-seq of xenografts generated by lung metastasis identified long noncoding RNA small nucleolar RNA host gene 10 (SNHG10) and its homolog SCARNA13 as novel drivers for the development and metastasis of HCC. SNHG10 expression positively correlated with SCARNA13 expression in 64 HCC cases, and high expression of SNHG10 or SCARNA13 was associated with poor overall survival. As SCARNA13 showed significant rise and decline after overexpression and knockdown of SNHG10, respectively, we hypothesized that SNHG10 might act as an upstream regulator of SCARNA13. SNHG10 and SCARNA13 coordinately contributed to the malignant phenotype of HCC cells, where SNHG10 served as a sponge for miR-150-5p and interacted with RPL4 mRNA to increase the expression and activity of c-Myb. Reciprocally, upregulated and hyperactivated c-Myb enhanced SNHG10 and SCARNA13 expression by regulating SNHG10 promoter activity, forming a positive feedback loop and continuously stimulating SCARNA13 expression. SCARNA13 mediated SNHG10-driven HCC cell proliferation, invasion, and migration and facilitated the cell cycle and epithelial–mesenchymal transition of HCC cells by regulating SOX9. Overall, we identified a complex circuitry underlying the concomitant upregulation of SNHG10 and its homolog SCARNA13 in HCC in the process of hepatocarcinogenesis and metastasis.Significance:These findings unveil the role of a noncoding RNA in carcinogenesis and metastasis of hepatocellular carcinoma.
miR-450a Acts as a Tumor Suppressor in Ovarian Cancer by Regulating Energy Metabolism
Authors: Muys Bruna Rodrigues , Sousa Josane F. , Plaça Jessica Rodrigues , de Araújo Luíza Ferreira , Sarshad Aishe A. , Anastasakis Dimitrios G. , Wang Xiantao , Li Xiao Ling , de Molfetta Greice Andreotti , Ramão Anelisa , Lal Ashish , Vidal Daniel Onofre , Hafner Markus , Silva Wilson A. 
Published: May 2019

AbstractDysregulation of miRNA expression is associated with multiple diseases, including cancers, in which small RNAs can have either oncogenic or tumor suppressive functions. Here we investigated the potential tumor suppressive function of miR-450a, one of the most significantly downregulated miRNAs in ovarian cancer. RNA-seq analysis of the ovarian cancer cell line A2780 revealed that overexpression of miR-450a suppressed multiple genes involved in the epithelial-to-mesenchymal transition (EMT). Overexpression of miR-450a reduced tumor migration and invasion and increased anoikis in A2780 and SKOV-3 cell lines and reduced tumor growth in an ovarian tumor xenographic model. Combined AGO-PAR-CLIP and RNA-seq analysis identified a panel of potential miR-450a targets, of which many, including TIMMDC1, MT-ND2, ACO2, and ATP5B, regulate energetic metabolism. Following glutamine withdrawal, miR-450a overexpression decreased mitochondrial membrane potential but increased glucose uptake and viability, characteristics of less invasive ovarian cancer cell lines. In summary, we propose that miR-450a acts as a tumor suppressor in ovarian cancer cells by modulating targets associated with glutaminolysis, which leads to decreased production of lipids, amino acids, and nucleic acids, as well as inhibition of signaling pathways associated with EMT.Significance:miR-450a limits the metastatic potential of ovarian cancer cells by targeting a set of mitochondrial mRNAs to reduce glycolysis and glutaminolysis.
Changes in DNA Level of Oncogenic Human Papillomaviruses Other Than Types 16 and 18 in Relation to Risk of Cervical Intraepithelial Neoplasia Grades 2 and 3
Authors: Xi Long Fu , Schiffman Mark , Hughes James P. , Galloway Denise A. , Koutsky Laura A. , Kiviat Nancy B. 
Published: May 2019

Abstract

Background:
Epidemiologic data addressing clinical relevance of viral load fluctuation of oncogenic types other than human papillomavirus (HPV) types 16 and 18 are limited.

Methods:
A type-stratified set of infections by non-HPV16/18 oncogenic types that were detected at ≥2 visits was randomly selected from women who were enrolled in a clinical trial and followed every 6 months for 2 years for detection of HPV and cervical intraepithelial neoplasia grades 2 and 3 (CIN2/3). Type-specific viral load was measured on both first and last HPV-positive cervical swab samples.

Results:
CIN2/3 was initially confirmed at the last HPV-positive visit for 67 of 439 infections. The increase in risk of CIN2/3 was associated with high, relative to low, viral load at both first and last positive visits [ORadjusted = 3.67; 95% confidence interval (CI), 1.19–11.32] and marginally associated with a change of viral load from low to high levels (ORadjusted = 3.15; 95% CI, 0.96–10.35) for infection by species group alpha-9 non-HPV16 oncogenic types but not species group alpha-5-7 non-HPV18 oncogenic types. Among women with an initial diagnosis of CIN2/3 at the first positive visit, CIN2/3 was more frequently redetected at the last positive visit for infections with, compared with without, high DNA load of species group alpha-9 non-HPV16 oncogenic types at both visits (Pexact = 0.04).

Conclusions:
In agreement with data on baseline viral load, the viral load change–associated risk of CIN2/3 differs by HPV species groups.

Impact:
These findings underscore the importance of distinguishing species groups in future studies of clinical relevance of HPV DNA load.
Rationale and Design of the VITALITY-HFpEF Trial
Authors: Butler Javed , Lam Carolyn S.P. , Anstrom Kevin J. , Ezekowitz Justin , Hernandez Adrian F. , O’Connor Christopher M. , Pieske Burkert , Ponikowski Piotr , Shah Sanjiv J. , Solomon Scott D. , Voors Adriaan A. , Wu Yi , Carvalho Francine , Bamber Luke , Blaustein Robert O. , Roessig Lothar , Armstrong Paul W. 
Published: May 2019

Background
The VITALITY-HFpEF trial (Evaluate the Efficacy and Safety of the Oral sGC Stimulator Vericiguat to Improve Physical Functioning in Daily Living Activities of Patients With Heart Failure and Preserved Ejection Fraction) is designed to determine the efficacy and safety of a novel oral soluble guanylate cyclase stimulator, vericiguat, on quality of life and exercise tolerance in heart failure patients with preserved ejection fraction (HFpEF). Impaired physical functioning reduces the quality of life in patients with HFpEF. The primary goal of HF treatment along with improving survival is to improve function, reduce symptoms, and maximize quality of life. Abnormal cyclic guanosine monophosphate signaling may contribute to physical limitations in patients with HFpEF via central and peripheral mechanisms. Exploratory post hoc analyses from a prior trial showed that vericiguat can improve patient-relevant domains of the Kansas City Cardiomyopathy Questionnaire, especially the physical limitation score.

Methods and Results
VITALITY-HFpEF is a placebo-controlled, double-blind, multi-center, phase IIb trial of ≈735 patients, ≥45 years with HFpEF and ejection fraction ≥45% who will be randomized 1:1:1 to placebo, 10 mg, or 15 mg vericiguat. The primary end point is change in Kansas City Cardiomyopathy Questionnaire physical limitation score from baseline to week 24 and change in 6-minute walk test from baseline to week 24 is the secondary end point.

Conclusions
VITALITY-HFpEF is the first trial designed to assess the efficacy of vericiguat in patients with HFpEF using the Kansas City Cardiomyopathy Questionnaire physical limitation score as a novel primary end point. This study will also extend the prior dosing experience with vericiguat in HF by studying the safety and efficacy of a 15 mg dose.

Clinical Trial Registration

URL:
https://www.clinicaltrials.gov
. Unique identifier: NCT03547583.
Governing the Central Mediterranean through Indirect Rule: Tracing the Effects of the Recognition of Joint Rescue Coordination Centre Tripoli
Authors: Santer Kiri 
Published: May 2019

Abstract
The gradual empowerment of the Libyan Coast Guard through EU training and funding has introduced them as a new actor in the Central Mediterranean amongst other civil and military actors intervening to prevent loss of life at sea. This article examines the contested recognition of the authority of the Joint Rescue Coordination Centre Tripoli over the newly formalized Libyan Search and Rescue Region. It argues that the recognition of the Libyan coordination authority made by the International Maritime Organisation, has changed the way the international waters separating Libya and Europe are governed. Through the close analysis of three ethnographic vignettes depicting instances of rescue of migrants by an NGO vessel, this article illustrates how the Italian authorities are able to exercise control over this vast area indirectly via the formalization of the Libyan authority and concomitantly imped the operations of civil rescue NGO boats in the zone. This formalization enables Italian authorities (and their EU counterparts) to establish a form of indirect governance in this liminal border zone that clashes with other preceding legal orders which regulate distress cases at sea, i.e. international maritime law.
Systématique et Répartition de Vipera Ursinii (Bonaparte, 1835) (Reptilia, Viperidae), en Roumanie
Authors: Vancea St. , Saint Girons H. , Fuhn I. E. , Stugren B. 
Published: May 2019

L’etude de la répartition et de la morphologie comparée de Vipera ursinii en Roumanie apporte les résultats suivants:
1. Les populations de Fînatele Clujului, maintenant disparues, appartenaient à la sous-espèce V. ursinii rakosiensis.
2. Les populations de Moldavie, qui présentent un mélange de caractères de V. ursinii rakosiensis et de V. ursinii renardi, correspondent à une zone d’intergradation secondaire.
3. Les populations du delta du Danube, également plus ou moins intermédiaires entre ces deux sous-especès, présentent en outre quelques caractères particuliers. En attendant de nouvelles recherches, il paraît cependant plus sage de leur conserver leur statut actuel de V. u. renardi.
Reduction in Northern Midlatitude 2-m Temperature Variability due to Arctic Sea Ice Loss
Authors: Collow Thomas W. , Wang Wanqiu , Kumar Arun 
Published: May 2019

Abstract
In this study, we investigate links between Arctic sea ice loss and the variability of 2-m temperatures over a 6-month period (November–April) over two domains centered over northern Eurasia and northern North America. Based on data from the Climate Forecast System Reanalysis (CFSR), there has been an increase (a decrease) in recent seasonal temperature variability over Eurasia (North America), which can be attributed to cooling (warming) during the winter months. Decreases in the intraseasonal variability of temperature anomalies, however, are noted in both regions for the November–April period. This study investigates the role of different forcings on the changes seen in the reanalysis product using Atmospheric Model Intercomparison Project simulations forced with repeating sea surface temperature, sea ice, and carbon dioxide concentration relative to climatologies from two different base periods, 1981–90 and 2005–14. The seasonal temperature and intraseasonal anomaly variabilities are examined, and we find that only the simulations with reduction in sea ice (2005–14 base-period sea ice concentration) produce significant decreases in intraseasonal temperature anomaly variability over these regions, agreeing with the CFSR analysis. Runs that reduce sea ice also result in a significant decrease in the frequency and magnitude of extreme warm and cold temperature anomalies. It is proposed that the weakened latitudinal temperature gradient, resulting from decreased sea ice, leads to reduced meridional temperature advection variability, which in turn contributes to the reduction in the variability of temperature anomalies.
Lagrangian Description of Air Masses Associated with Latent Heat Release in Tropical Storm Karl (2016) during Extratropical Transition
Authors: Euler Christian , Riemer Michael , Kremer Tobias , Schömer Elmar 
Published: May 2019

Abstract
Extratropical transition (ET) of tropical cyclones involves distinct changes of the cyclone’s structure that are not yet well understood. This study presents for the first time a comprehensive Lagrangian description of structure change near the inner core. A large sample of trajectories is computed from a convection-permitting numerical simulation of the ET of Tropical Storm Karl (2016). Three main airstreams are considered: those associated with the inner-core convection, inner-core descent, and the developing warm conveyor belt. Analysis of these airstreams is performed both in thermodynamic and physical space. Prior to ET, Karl is embedded in weak vertical wind shear and its intensity is impeded by excessive detrainment from the inner-core convection. At the start of ET, vertical shear increases and Karl intensifies, which is attributable to reduced detrainment and thus to the formation of a well-defined outflow layer. During ET, the thermodynamic changes of the environment impact Karl’s inner-core convection predominantly by a decrease of θe values in the inflow layer. Notably, notwithstanding Karl’s weak intensity, its inner core acts as a “containment vessel” that transports high-θe air into the increasingly hostile environment. Inner-core descent has two origins: (i) mostly from upshear-left above 4-km height in the environment and (ii) boundary layer air that ascends in the inner core first and then descends, performing rollercoaster-like trajectories. At the end of the tropical phase of ET, the developing warm conveyor belt comprises air masses from several different source regions, and only partly from the cyclone’s developing warm sector, as expected for extratropical cyclones.
Atmospheric Composite Sounding Analysis as a Forecasting Tool for Ozone Accumulation in a Semiarid Metropolitan Area during the North America Monsoon: Phoenix, Arizona
Authors: Malloy Jonny William , Cerveny Randall S. 
Published: May 2019

Abstract
Phoenix, Arizona, observes a high summertime frequency for daily maximum 8-h ozone averages (DMO8) exceeding 70 parts per billion, resulting in nonattainment status by the U.S. Environmental Protection Agency. This study discusses the use of composite atmospheric sounding analysis (constructing average sounding conditions for specific recurring events) to forecast different air quality index daily ozone classifications, including “good,” “moderate,” and collectively categories exceeding the 2015 ozone standard. Composite sounding analysis, using the Phoenix 1200 UTC (0500 LST) rawinsonde data (2006–17), identifies “pollutant dispersion windows” for ozone accumulation or dispersal for Phoenix during the North American monsoon (July and August period). A favorable ozone exceedance atmosphere is associated with a “Four Corners high” synoptic pattern bringing relatively light winds at and below 700 hPa (≤4.5 ms−1) and higher easterly winds above (≤12.3 ms−1). Healthy ozone days (good category) are common when Pacific low pressure troughing over the western United States creates deep and faster westerly flow above the surface reaching speeds of 6.7 (19.5) m s−1 by 700 (200) hPa. Surprisingly, large standard deviations over 10°C for dewpoint temperature at midlevels (500–400 hPa) were determined for all three daily ozone classification ranges used in this study. Additionally, modest temperature deviations and mean differences are noted at significant pressure levels. Consequently, wind speed and direction are better indicators when forecasting ozone accumulation potential. These results are pertinent to air quality meteorologists responsible for disseminating ozone forecasts for heavily urbanized areas of the U.S. Southwest.
The prognostic values of matrix metalloproteinases in ovarian cancer
Authors: Zeng Linchai , Qian Jie , Zhu Fangfang , Wu Fang , Zhao Hongqin , Zhu Haiyan 
Published: May 2019

Objective To investigate the prognostic significance of 23 matrix metalloproteinase ( MMP) genes in patients diagnosed with ovarian carcinoma. Methods The prognostic significance of 23 MMP genes in patients diagnosed with ovarian carcinoma was investigated using the Kaplan–Meier plotter (KM plotter), which uses the gene expression data and overall survival information of patients with ovarian cancer that were downloaded from the Gene Expression Omnibus, Cancer Biomedical Informatics Grid and The Cancer Genome Atlas cancer datasets. The correlation between mRNA levels of individual MMPs (MMP2, MMP9, MMP10, MMP12, MMP13 and MMP25) and clinicopathological features (histological subtype, pathological grade and clinical stage) were investigated. The MMP protein level profiles in normal ovarian tissues and ovarian cancer tissues were examined using the Human Protein Atlas database. Results The results showed that high mRNA levels of MMP2 and MMP13 were associated with a worse overall survival in patients with ovarian cancer, whereas high mRNA levels of MMP9, MMP10, MMP12 and MMP25 were associated with a better overall survival. The protein levels of MMP2, MMP9, MMP10 and MMP25 in ovarian cancer tissues were elevated compared with normal ovarian tissues. Conclusions This study demonstrated that MMPs can be a reliable prognostic biomarker for ovarian cancer.
Osteochondral Allograft Transplantation of the Femoral Condyle Utilizing a Thin Plug Graft Technique
Authors: Tírico Luís E.P. , McCauley Julie C. , Pulido Pamela A. , Bugbee William D. 
Published: May 2019

Background:Previous studies showed clinical benefit and durable results of osteochondral allograft (OCA) transplantation for the treatment of femoral condyle lesions. However, the majority of these studies are difficult to interpret owing to the mixed results of different techniques and anatomic locations.Purpose:To evaluate the outcome of OCA transplantation with thin plug grafts for treatment of isolated femoral condyle osteochondral lesions.Study Design:Case series; Level of evidence, 4.Methods:This study included 187 patients (200 knees) who underwent OCA transplantation for isolated osteochondral lesions on the femoral condyle between 1999 and 2014. For all cases, a thin plug technique was used with commercially available surgical instruments and the minimum amount of bone necessary for fixation. Evaluation included International Knee Documentation Committee score, Knee injury and Osteoarthritis Outcome Score, and patient satisfaction. Frequency and type of further surgery were assessed. Failure of the allograft was defined as further surgery involving removal of the allograft.Results:Mean follow-up was 6.7 years (range, 1.9-16.5 years). The mean age of patients at the time of surgery was 31 years, and 63% were male. The medial femoral condyle was affected in 69% of knees. A single thin plug graft was used in 145 knees (72.5%), and 2 grafts were used in 55 knees (27.5%). Mean allograft area was 6.3 cm2, and graft thickness was 6.5 mm (cartilage and bone combined). Further surgery was required for 52 knees (26%), of which 16 (8% of entire cohort) were defined as allograft failures (4 OCA revisions, 1 arthrosurface, 6 unicompartmental knee arthroplasties, and 5 total knee arthroplasties). Median time to failure was 4.9 years. Survivorship of the allograft was 95.6% at 5 years and 91.2% at 10 years. Among patients with grafts remaining in situ at latest follow-up, clinically meaningful improvement in pain, function, and quality of life was reported. Satisfaction was reported by 89% of patients.Conclusion:OCA transplantation with a thin plug graft technique is a valuable procedure for the treatment of femoral condyle osteochondral lesions, resulting in significant improvement in clinical scores, high patient satisfaction, and low reoperation and clinical failure rates.