bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complications after pelvic floor repair surgery (with and without mesh): short‐term incidence after 1873 inclusions in the French VIGI‐MESH registry
Authors: Fritel X , Campagne‐Loiseau S , Cosson M , Ferry P , Saussine C , Lucot J‐P , Salet‐Lizee D , Barussaud M‐L , Boisramé T , Carlier‐Guérin C , Charles T , Debodinance P , Deffieux X , Pizzoferrato A‐C , Curinier S , Ragot S , Ringa V , de Tayrac R , Fauconnier A 
Published: September 2019

ObjectiveTo assess the short‐term incidence of serious complications of surgery for urinary incontinence or pelvic organ prolapse.DesignProspective longitudinal cohort study using a surgical registry.SettingThirteen public hospitals in France.PopulationA cohort of 1873 women undergoing surgery between February 2017 and August 2018.MethodsPreliminary analysis of serious complications after a mean follow‐up of 7 months (0–18 months), according to type of surgery. Surgeons reported procedures and complications, which were verified by the hospitals’ information systems.Main outcome measuresSerious complication requiring discontinuation of the procedure or subsequent surgical intervention, life‐threatening complication requiring resuscitation, or death.ResultsFifty‐two women (2.8%, 95% CI 2.1–3.6%) experienced a serious complication either during surgery, requiring the discontinuation of the procedure, or during the first months of follow‐up, necessitating a subsequent reoperation. One woman also required resuscitation; no women died. Of 811 midurethral slings (MUSs), 11 were removed in part or totally (1.4%, 0.7–2.3%), as were two of 391 transvaginal meshes (0.5%, 0.1–1.6%), and four of 611 laparoscopically placed mesh implants (0.7%, 0.2–1.5%). The incidence of serious complications 6 months after the surgical procedure was estimated to be around 3.5% (2.0–5.0%) after MUS alone, 7.0% (2.8–11.3%) after MUS with prolapse surgery, 1.7% (0.0–3.8%) after vaginal native tissue repair, 2.8% (0.9–4.6%) after transvaginal mesh, and 1.0% (0.1–1.9%) after laparoscopy with mesh.ConclusionsEarly serious complications are relatively rare. Monitoring must be continued and expanded to assess the long‐term risk associated with mesh use and to identify its risk factors.Tweetable abstractShort‐term serious complications are rare after surgery for urinary incontinence or pelvic organ prolapse, even with mesh.
Peanut Oil Body Composition and Stability
Authors: Zhou Long‐zheng , Chen Fu‐sheng , Hao Li‐hua , Du Yan , Liu Chen 
Published: September 2019

AbstractThis study was aimed to assess the effect of membrane structure on the stability of peanut oil bodies extracted by enzyme‐assisted extraction. The influence of pH, NaCl concentration, and temperature on the physicochemical properties of peanut oil bodies was characterized using ζ‐potential and particle size. The results indicated that the peanut oil bodies had strong stability (ζ‐potential, >20 mV) at pH values away from the isoelectric point (pH 4.8), at a low salt concentration (NaCl concentration, <10 mM), and in a certain temperature range (35 to 55 °C). The stable structure of the oil body was closely related to its structure. Phospholipids, along with membrane proteins, were major components of the oil body membrane. Therefore, the phospholipid composition and content were measured and the types of membrane proteins of the oil bodies were identified. The results showed that phosphatidylcholine and phosphatidylserine were major components of the oil body phospholipids. Two‐dimensional electrophoresis showed that the oil bodies contained both intrinsic proteins and extrinsic proteins, which might play an important role in the stability of oil bodies during enzyme‐assisted extraction processing.
Identification and Antibiotic Resistance Assessment of Ensifer adhaerens YX1, a Vitamin B12‐Producing Strain Used as a Food and Feed Additive
Authors: Zhao Ting , Cheng Kun , Cao Yan‐hua , Ouwehand Arthur C. , Jiao Cui‐feng , Yao Su 
Published: September 2019

AbstractThis study provides phenotypic and molecular analyses of the antibiotic resistance of Ensifer adhaerens strain YX1 (CICC 11008s), a strain that was identified using a polyphasic taxonomy approach. The antibiotic resistance profile of E. adhaerens YX1 was assessed using the Clinical & Laboratory Standards Inst. (CLSI) method. The strain was susceptible to ciprofloxacin, levofloxacin, norfloxacin, ofloxacin, gentamicin, tobramycin, chloramphenicol, tetracycline, imipenem, and ceftazidime, and resistant to kanamycin, streptomycin, fosfomycin, and nitrofurantoin. The antibiotic resistance genes nsfA, nsfB, fosA, aph, and aadA1 were not detected in E. adhaerens YX1 via PCR using gene‐specific primers. Subsequently, the genome sequence of E. adhaerens was screened for antibiotic genes. Although no antibiotic resistance genes were identified using the ResFinder database, five genes copies of one resistance gene, adeF, were detected using the Comprehensive Antibiotic Resistance Database (CARD). The results of this study will be useful for understanding the phenotypic and genotypic aspects of E. adhaerens antibiotic resistance. No safety issues were identified for E. adhaerens YX1 in terms of antibiotic resistance. Performing similar studies will be conducive to the safety assessment and control of the use of E. adhaerens in the food and feed industry.Practical ApplicationFew relevant reports are currently available regarding antibiotic resistance assessments or other safety evaluations for Ensifer adhaerens. Because of a lack of relevant information on the safety of this bacterium, including the genetic basis of antibiotic resistance in the production strain, it has not been recommended for use in the “qualified presumption of safety” (QPS) list and subsequent updated lists. The current study shows no safety issue of E. adhaerens YX1 in terms of its antibiotic resistance. These results are important as they provide an initial basis for an understanding of the antibiotic resistance/susceptibility of E. adhaerens YX1 (CICC 11008s), which produces vitamin B12 and is widely used in the food and feed industry.
Effects of Visual Cues on Consumer Expectation, Emotion and Wellness Responses, and Purchase Intent of Red Chili Powders
Authors: Ngoenchai Phurit , Alonso Jose Ramon , Suwonsichon Thongchai , Suwonsichon Suntaree , Prinyawiwatkul Witoon 
Published: September 2019

AbstractThe effects of visual cues on familiarity, expected heat intensity, liking of appearance, emotional and wellness responses, and purchase intent (PI) before and after disclosing information associated with red chili powders were determined using a 3‐point scale, a 15‐cm line scale, a 9‐point hedonic scale, a 15‐cm line scale, and a binomial scale, respectively. In this study, consumers only visually evaluated red chili powder samples without sniffing nor tasting. Eight chili powders were prepared according to a 23 factorial design: roasted (Ro) compared with unroasted (Un); whole pod with seeds (Wh) compared with seedless (Sl); coarsely (Cr) compared with finely ground (Gr). Thai consumers (N = 230) were generally more familiar with samples having coarse particles and more reddish color (lower hue angle and higher a* values) than samples having finely ground particles and less reddish/more yellowish color (higher hue angle and lower a* values). The expected heat intensity and liking scores for appearance were lower for samples with higher hue values, particularly RoWhGr and RoSIGr samples. All scores for emotion/wellness terms, except curious, were generally higher for samples with lower hue angle and higher a* values (redness). The consumer familiarity to the appearance of the samples influenced expected heat intensity, liking of appearance, and emotion/wellness responses. PI increased by >10% after presenting “organic,” “aflatoxin free,” and “organic and aflatoxin free” product statements to consumers. Results showed that familiarity, overall liking of appearance, color liking, fine particles liking, and healthy as well as wild terms were significant predictors for PI (odds ratio = 1.282, 1.519, 1.314, 1.158, 1.056, and 0.939, respectively) of red chili powders.Practical ApplicationThis study showed that consumer familiarity to the appearance (visual cues) of red chili powder affected expected heat intensity, liking of appearance, and emotion/wellness responses, which, in turn, affected purchase intent (PI). Samples with more reddish color and coarse particles were perceived to be more familiar than those with more yellowish color and finer particles. Familiarity, overall liking of appearance, color liking, fine particles liking, and healthy and wild terms were significant predictors for PI of chili power. The results demonstrated the importance of visual cues on the consumers’ expectation and PI of red chili powder, thus offering valuable information for manufacturers.
Characteristics of retinal layer thickness in acute anterior uveitis: an optical coherence tomography study
Authors: Lee Min‐Woo , Lee Taek‐Hoon , Won Yeo‐Kyoung , Shin Yong‐Il , Kim Jung‐Yeul 
Published: September 2019

AbstractPurposeTo determine the changes in peripapillary retinal nerve fiber layer (pRNFL), macular and ganglion cell‐inner plexiform layer (GC‐IPL) thicknesses in patients with acute anterior uveitis (AAU).MethodsPatients diagnosed with unilateral non‐infectious AAU and normal control were enrolled retrospectively. Optical coherence tomography scans were performed during the initial active phase and inactive phase of AAU. Patients were followed for at least 3 months after resolution of inflammatory activity.ResultsThirty‐seven AAU patients and 40 controls were included. The average RNFL and central macular thickness (CMT) showed significant differences between patients and control groups during active phase (p < 0.001 and p = 0.022, respectively). The average pRNFL thicknesses of affected eyes and fellow eyes during the active phase were 109.4 ± 12.5 μm and 96.5 ± 8.0 μm, respectively (p < 0.001). During the inactive phase, the average thicknesses were 99.3 ± 8.9 μm and 97.0 ± 7.5, respectively; they were not significantly different (p = 0.236). The CMTs of affected and fellow eyes during the active phase were 261.6 ± 24.7 μm and 251.5 ± 17.3 μm, respectively; the difference was significant (p = 0.047). The average GC‐IPL thicknesses of affected and fellow eyes were not significantly different (p = 0.061).ConclusionsThe pRNFL and central macula thickened during the active phase of AAU and decreased to a similar degree with fellow eyes during the inactive phase. Additionally, pRNFL thickness showed a sensitive response to the degree of inflammation in AAU. This suggests that pRNFL thickness may be useful in assessing disease activity.
Retinal vascular fractal dimension and cerebral blood flow, a pilot study
Authors: Nadal Jeremy , Deverdun Jeremy , de Champfleur Nicolas Menjot , Carriere Isabelle , Creuzot‐Garcher Catherine , Delcourt Cécile , Chiquet Christophe , Kawasaki Ryo , Villain Max , Ritchie Karen , Le Bars Emmanuelle , Daien Vincent 
Published: September 2019

AbstractPurposeOcular and brain microcirculation share embryological and histological similarities. The retinal vascular fractal dimension (FD) is a marker of retinal vascular complexity of the vascular tree. The purpose of this study was to explore the relationship between cerebral blood flow (CBF), retinal vascular FD and other retinal vascular markers.MethodsCross‐sectional analysis comprising 26 individuals ≥65 years old from the Cognitive REServe and Clinical ENDOphenotype (CRESCENDO) cohort of relative healthy older adults. Retinal vascular FD was measured from fundus photographs by using the semi‐automated Singapore Eye Vessel Assessment (SIVA) software. CBF was estimated using a 2D pulsed ASL MRI sequence. Associations between blood flow and retinal parameters were analysed using linear regression models adjusted for age and sex.ResultsCerebral blood flow was positively associated with venular FD (R2 = 0.32, p = 0.03). This association was stronger in the anterior versus posterior brain territories (R2 = 0.35 [p = 0.001] versus R2 = 0.16 [p = 0.07], respectively). Global CBF was correlated with arteriolar branching angle (R2 = 0.23, p = 0.01) and tortuosity (R2 = 0.20, p = 0.02). Global CBF was not correlated with other SIVA parameters.ConclusionsRetinal venular complexity summarized by the FD was associated with cerebral blood flow as well as retinal arteriolar tortuosity and branching angle. Larger prospective clinical studies are needed to confirm these results.
ESSENCE‐Q obtained in routine Japanese public child health check‐ups may be a valuable tool in neurodevelopmental screening
Authors: Hatakenaka Yuhei , Maeda Masato , Ninomiya Hitoshi , Hachiya Koutarou , Fernell Elisabeth , Gillberg Christopher 
Published: September 2019

AbstractAimOur aim was to extend the validity of a questionnaire developed for screening and identifying early symptomatic syndromes eliciting neurodevelopmental clinical examinations‐questionnaire (ESSENCE‐Q) in young children.MethodsEarly symptomatic syndromes eliciting neurodevelopmental clinical examinations‐questionnaire data for 207 children, living in Aki City, Japan, in 2014‐2015, were obtained from mothers, public health nurses and psychologists at 20‐ and 40‐month routine check‐ups at child healthcare centres. These were checked against subsequent ESSENCE diagnoses made by physicians. Receiver operating characteristic curves were constructed, and the area under the curves was compared. Sensitivity, specificity, positive predictive values (PPVs) and negative predictive values were calculated at optimal cut‐off values. The clinical utility index was also calculated.ResultsWhen the ESSENCE‐Q was used by public health nurses, it demonstrated good validity, in terms of high sensitivity and high NPVs, at the 20‐month check‐up, but not at 40 months. Psychologists demonstrated good validity at both ages, but mothers did not. Good negative utility indexes, indicating screening accuracy, were obtained from the psychologists at both check‐ups and from nurses at 20 months.ConclusionThe ESSENCE‐Q results used by nurses and psychologists showed good validity. Future studies should confirm the effectiveness of this tool to identify children in need of clinical detailed neurodevelopmental assessment.
The recovery rates of secondary savannas in abandoned pastures are poorly explained by environmental and landscape factors
Authors: Cava Mário G.B. , Pilon Natashi A.L. , Priante Camila F. , Ribeiro Milton Cezar , Durigan Giselda 
Published: September 2019

AbstractQuestionAssessing the natural regeneration potential of degraded savannas is a crucial step in restoration planning, since that assessment will define the need for and costs of active intervention. Predicting natural regeneration, however, depends on the mechanistic understanding of ecosystem resilience. Here, we searched for the factors modulating plant communities spontaneously regenerating in abandoned pastures.LocationTropical savanna (cerrado), Brazil.MethodsOver two years, we quantified changes in the structure, richness and species composition of plant communities in 29 secondary savannas resulting from pasture abandonment. We then investigated the influence of soil and landscape attributes, exotic grass cover and time since pasture abandonment on the recovery rate and species composition of these communities.ResultsThe wide variation among sites was not explained by time since abandonment or distance to the nearest remnant native vegetation. Soil attributes, exotic grasses and native vegetation cover around a pasture explained a small fraction of the variation in the recovery rate. We did not find an isolated factor or a robust set of factors explaining the variation in the recovery rate for all vegetation attributes. Species composition was slightly influenced by exotic grasses, soil penetration resistance, proportion of fine soil particles and time since abandonment. Colonization and resprouting by savanna specialists over the two‐year period were hindered by exotic grasses.ConclusionThe use of predictive models based on soil properties, exotic grasses, landscape attributes or time since abandonment is unfeasible for inferring the recovery rate of savanna structure and richness after pasture abandonment. Case‐by‐case monitoring is required to support decisions on restoration intervention. Other factors, which are almost impossible to obtain, such as land‐use practices and history and the taxonomic or functional composition of pre‐existing vegetation, can potentially influence the recovery rate. Exotic grasses must be controlled to favour colonization and resprouting by savanna specialists.
Analysis of secondary phenotypes in multigroup association studies
Authors: Zhou Fan , Zhou Haibo , Li Tengfei , Zhu Hongtu 
Published: September 2019

AbstractAlthough case‐control association studies have been widely used, they are insufficient for many complex diseases, such as Alzheimer's disease and breast cancer, since these diseases may have multiple subtypes with distinct morphologies and clinical implications. Many multigroup studies, such as the Alzheimer's Disease Neuroimaging Initiative (ADNI), have been undertaken by recruiting subjects based on their multiclass primary disease status, while extensive secondary outcomes have been collected. The aim of this paper is to develop a general regression framework for the analysis of secondary phenotypes collected in multigroup association studies. Our regression framework is built on a conditional model for the secondary outcome given the multigroup status and covariates and its relationship with the population regression of interest of the secondary outcome given the covariates. Then, we develop generalized estimation equations to estimate the parameters of interest. We use both simulations and a large‐scale imaging genetic data analysis from the ADNI to evaluate the effect of the multigroup sampling scheme on standard genome‐wide association analyses based on linear regression methods, while comparing it with our statistical methods that appropriately adjust for the multigroup sampling scheme. Data used in preparation of this article were obtained from the ADNI database.
Laryngeal dyskinesia in a horse
Authors: Kühnle C. , Kümmerle J. M. , Parente E. J. , Weishaupt M. A. 
Published: September 2019

SummaryA 3‐year‐old Warmblood gelding was evaluated for intermittent respiratory distress and an inspiratory noise. These signs were mainly observed at exercise but sometimes occurred at rest as well. At the age of 2 months, a severe episode of respiratory distress had necessitated a temporary tracheotomy, which had then healed uneventfully. Examination of the patient and resting endoscopy revealed no structural laryngeal alterations. Exercise endoscopy revealed episodes of active bilateral laryngeal adduction during inspiration. Spirometrically, a corresponding impairment of inspiratory airflow was identified. These abnormalities were most pronounced at the trot but they occurred at magnitudes of inspiratory airflow lower than those inducing dynamic arytenoid collapse in horses with recurrent laryngeal neuropathy. Therefore, the diagnosis of a laryngeal dyskinesia characterised by an active bilateral arytenoid adduction during inspiration was made. No specific treatment was implemented. Three years later, the owner reported that the condition was markedly improved and the horse was used for pleasure riding.
The modular structure of α/β‐hydrolases
Authors: Bauer Tabea L. , Buchholz Patrick C. F. , Pleiss Jürgen 
Published: September 2019

The α/β‐hydrolase fold family is highly diverse in sequence, structure and biochemical function. To investigate the sequence–structure–function relationships, the Lipase Engineering Database (https://led.biocatnet.de) was updated. Overall, 280 638 protein sequences and 1557 protein structures were analysed. All α/β‐hydrolases consist of the catalytically active core domain, but they might also contain additional structural modules, resulting in 12 different architectures: core domain only, additional lids at three different positions, three different caps, additional N‐ or C‐terminal domains and combinations of N‐ and C‐terminal domains with caps and lids respectively. In addition, the α/β‐hydrolases were distinguished by their oxyanion hole signature (GX‐, GGGX‐ and Y‐types). The N‐terminal domains show two different folds, the Rossmann fold or the β‐propeller fold. The C‐terminal domains show a β‐sandwich fold. The N‐terminal β‐propeller domain and the C‐terminal β‐sandwich domain are structurally similar to carbohydrate‐binding proteins such as lectins. The classification was applied to the newly discovered polyethylene terephthalate (PET)‐degrading PETases and MHETases, which are core domain α/β‐hydrolases of the GX‐ and the GGGX‐type respectively. To investigate evolutionary relationships, sequence networks were analysed. The degree distribution followed a power law with a scaling exponent γ = 1.4, indicating a highly inhomogeneous network which consists of a few hubs and a large number of less connected sequences. The hub sequences have many functional neighbours and therefore are expected to be robust toward possible deleterious effects of mutations. The cluster size distribution followed a power law with an extrapolated scaling exponent τ = 2.6, which strongly supports the connectedness of the sequence space of α/β‐hydrolases.
Database
Supporting data about domains from other proteins with structural similarity to the N‐ or C‐terminal domains of α/β‐hydrolases are available in Data Repository of the University of Stuttgart (DaRUS) under doi: https://doi.org/10.18419/darus-458.