bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endothelial Cell–Specific Inactivation of TSPAN12 (Tetraspanin 12) Reveals Pathological Consequences of Barrier Defects in an Otherwise Intact Vasculature
Authors: Zhang Chi , Lai Maria B. , Pedler Michelle G. , Johnson Verity , Adams Ralf H. , Petrash J. Mark , Chen Zhe , Junge Harald J. 
Published: October 2018

Objective—
Blood-CNS (central nervous system) barrier defects are implicated in retinopathies, neurodegenerative diseases, stroke, and epilepsy, yet, the pathological mechanisms downstream of barrier defects remain incompletely understood. Blood-retina barrier (BRB) formation and retinal angiogenesis require β-catenin signaling induced by the ligand norrin (NDP [Norrie disease protein]), the receptor FZD4 (frizzled 4), coreceptor LRP5 (low-density lipoprotein receptor-like protein 5), and the tetraspanin TSPAN12 (tetraspanin 12). Impaired NDP/FZD4 signaling causes familial exudative vitreoretinopathy, which may lead to blindness. This study seeked to define cell type-specific functions of TSPAN12 in the retina.

Approach and Results—

A loxP-flanked
Tspan12
allele was generated and recombined in endothelial cells using a tamoxifen-inducible Cdh5-CreERT2 driver. Resulting phenotypes were documented using confocal microscopy. RNA-Seq, histopathologic analysis, and electroretinogram were performed on retinas of aged mice. We show that TSPAN12 functions in endothelial cells to promote vascular morphogenesis and BRB formation in developing mice and BRB maintenance in adult mice. Early loss of TSPAN12 in endothelial cells causes lack of intraretinal capillaries and increased VE-cadherin (CDH5 [cadherin5 aka VE-cadherin]) expression, consistent with premature vascular quiescence. Late loss of TSPAN12 strongly impairs BRB maintenance without affecting vascular morphogenesis, pericyte coverage, or perfusion. Long-term BRB defects are associated with immunoglobulin extravasation, complement deposition, cystoid edema, and impaired b-wave in electroretinograms. RNA-sequencing reveals transcriptional responses to the perturbation of the BRB, including genes involved in vascular basement membrane alterations in diabetic retinopathy.

Conclusions—

This study establishes mice with late endothelial cell–specific loss of
Tspan12
as a model to study pathological consequences of BRB impairment in an otherwise intact vasculature.
Impaired HDL (High-Density Lipoprotein)-Mediated Macrophage Cholesterol Efflux in Patients With Abdominal Aortic Aneurysm—Brief Report
Authors: Martínez-López Diego , Cedó Lídia , Metso Jari , Burillo Elena , García-León Annabel , Canyelles Marina , Lindholt Jes S. , Torres-Fonseca Monica , Blanco-Colio Luis Miguel , Vázquez Jesús , Blanco-Vaca Francisco , Jauhiainen Matti , Martín-Ventura Jose Luis , Escolà-Gil Joan Carles 
Published: October 2018

Objective—
The ability of HDL (high-density lipoprotein) to promote macrophage cholesterol efflux is considered the main HDL cardioprotective function. Abdominal aortic aneurysm (AAA) is usually characterized by cholesterol accumulation and macrophage infiltration in the aortic wall. Here, we aim to evaluate the composition of circulating HDL particles and their potential for promoting macrophage cholesterol efflux in AAA subjects.

Approach and Results—
First, we randomly selected AAA and control subjects from Spain. The AAA patients in the Spanish cohort showed lower plasma apoA-I levels concomitantly associated with low levels of plasma HDL cholesterol and the amount of preβ-HDL particles. We determined macrophage cholesterol efflux to apoB-depleted plasma, which contains mature HDL, preβ-HDL particles and HDL regulatory proteins. ApoB-depleted plasma from AAA patients displayed an impaired ability to promote macrophage cholesterol efflux. Next, we replicated the experiments with AAA and control subjects derived from Danish cohort. Danish AAA patients also showed lower apoA-I levels and a defective HDL-mediated macrophage cholesterol efflux.

Conclusions—
AAA patients show impaired HDL-facilitated cholesterol removal from macrophages, which could be mechanistically linked to AAA.
Association Between Incomplete Circle of Willis and Carotid Vulnerable Atherosclerotic Plaques
Authors: Zhou Changwu , Yuan Chun , Li Rui , Wang Wei , Li Cheng , Zhao Xihai ,   on behalf of the CARE-II Study Collaborators
Published: October 2018

Objective—
Carotid high-risk plaque, characterized by intraplaque hemorrhage, fibrous cap rupture, and large lipid-rich necrotic core, is associated with cerebrovascular events. This study sought to investigate the relationship between high-risk carotid plaque and an incomplete circle of Willis (COW).

Approach and Results—

Patients were recruited from a multicenter study, Chinese Atherosclerosis Risk Evaluation (CARE-II) and underwent 3-dimensional time-of-flight magnetic resonance angiography for intracranial arteries and 2-dimensional multicontrast magnetic resonance vessel wall imaging for carotid arteries on a 3.0T magnetic resonance scanner. The integrity of the COW in anterior and posterior portions was evaluated. Characteristics of carotid plaques were assessed. Correlation between incomplete COW and carotid plaque features was determined. Of 482 eligible patients, patients with carotid intraplaque hemorrhage showed significantly higher prevalence of an incomplete anterior COW (52.7% versus 38.5%;
P
=0.022) compared with those without. An incomplete anterior COW was associated with intraplaque hemorrhage before (odds ratio, 1.781; 95% CI, 1.083–2.931;
P
=0.023) and after adjusted for clinical risk factors (odds ratio, 1.945; 95% CI, 1.139–3.321;
P
=0.015). The unilateral carotid artery stenosis showed no correlation with incomplete anterior COW and posterior COW (all
P
>0.025). No significant associations were found between other plaque features and any type of incomplete COW (all
P
>0.025).

Conclusions—
An incomplete COW is independently associated with intraplaque hemorrhage of carotid atherosclerotic plaques.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT02017756.
Development of an Instrument to Assess Work Productivity in Individuals With Voice Disorders: The Work Hoarse
Authors: Zhu Qiubei , Hu Amanda , Giliberto John Paul , Carlson Samantha , Jensen Samantha , Tiu Ryan , Meyer Tanya K. 
Published: October 2018

Objectives: Vocal function is critical to employability in the modern era. Although research clearly demonstrates that a disordered voice affects quality of life, few studies have attempted to quantitate the effects of a disordered voice on work productivity. The Voice-Related Statements battery, which originally had 20 items, was previously developed to qualitatively describe how an individual’s dysphonia affects his or her job performance. The aim of this study was to refine and reduce the item number and provide preliminary validation of this shortened instrument. Methods: The Voice-Related Statements instrument was administered to employed patients with dysphonia in conjunction with 2 additional instruments: the Voice Handicap Index (VHI) and the Work Productivity and Activity Impairment questionnaire (WPAI). Response distributions and intercorrelations were examined for item reduction. Exploratory factor analysis was conducted on retained items. Correlations were performed with other voice-related instruments. Reliability was tested using a coefficient of stability. Results: One hundred seventy-four employed patients with dysphonia were enrolled in this study. Six items were removed because of redundancy, factor analysis, and cognitive interview results. Exploratory factor analysis revealed a 1-factor structure explaining 66.28% of the total variance. The final 8-item instrument demonstrated good internal consistency (Cronbach α = 0.93), with a moderately robust correlation with the VHI ( r = 0.68, P < .001) and WPAI work impairment measures ( r = 0.63, P < .001). The VHI showed a much weaker correlation with WPAI work impairment ( r = 0.48, P < .001). Test-retest reliability was good (0.83, P < .01). Conclusions: An 8-item instrument to qualitatively measure the impact of a disordered voice at work has been developed and is called the Work Hoarse. This instrument is a better measure of voice-related work productivity impairment than the VHI and will augment quantitative work productivity instruments that are currently available.
Copeptin and insulin-like growth factor binding protein-1 during follow-up after an acute myocardial infarction in patients with type 2 diabetes: A report from the Diabetes Mellitus Insulin-Glucose Infusion in Acute Myocardial Infarction 2 cohort
Authors: Smáradóttir Maria Isabel , Catrina Sergiu-Bogdan , Brismar Kerstin , Norhammar Anna , Gyberg Viveca , Mellbin Linda G 
Published: October 2018

Purpose: Copeptin and insulin-like growth factor binding protein-1 analysed at admission for a myocardial infarction in patients with type 2 diabetes mellitus predicts cardiovascular events. The present aim was to study the association between copeptin and insulin-like growth factor binding protein-1, the development of the levels over time, and if the predictive value remained when measured at hospital discharge and 3 months thereafter. Methods: Copeptin and insulin-like growth factor binding protein-1 were analysed in patients (median age = 70, male = 68%) with type 2 diabetes mellitus + myocardial infarction at admission (n = 393), discharge (n = 309) and 3 months later (n = 288). The primary endpoint was cardiovascular event (cardiovascular death/non-fatal myocardial infarction/stroke) with the three time points as separate baselines. Results: The median copeptin levels were 21.8 pmol/L at admission, 8.5 pmol/L at discharge and 8.4 pmol/L after 3 months, while insulin-like growth factor binding protein-1 levels continued to increase. There were significant correlations between the biomarkers at all occasions. During an average follow-up of 2.5 years, copeptin, but not insulin-like growth factor binding protein-1, predicted cardiovascular event at all occasions in unadjusted analyses. Copeptin remained as a predictor at discharge and after 3 months in the final multiple model (including: heart failure/age/creatinine clearance). Conclusion: The relationship between copeptin and insulin-like growth factor binding protein-1 during the initial phase of a myocardial infarction persisted in a less-stressful situation, and copeptin remained as a prognostic indicator at discharge and 3 months later.
Updated Canadian Expert Consensus on Assessing Risk of Disease Progression and Pharmacological Management of Autosomal Dominant Polycystic Kidney Disease
Authors: Soroka Steven , Alam Ahsan , Bevilacqua Micheli , Girard Louis-Philippe , Komenda Paul , Loertscher Rolf , McFarlane Philip , Pandeya Sanjaya , Tam Paul , Bichet Daniel G. 
Published: October 2018

Purpose: The purpose of this article is to update the previously published consensus recommendations from March 2017 discussing the optimal management of adult patients with autosomal dominant polycystic kidney disease (ADPKD). This document focuses on recent developments in genetic testing, renal imaging, assessment of risk regarding disease progression, and pharmacological treatment options for ADPKD. Sources of information: Published literature was searched in PubMed, the Cochrane Library, and Google Scholar to identify the latest evidence related to the treatment and management of ADPKD. Methods: All pertinent articles were reviewed by the authors to determine if a new recommendation was required, or if the previous recommendation needed updating. The consensus recommendations were developed by the authors based on discussion and review of the evidence. Key findings: The genetics of ADPKD are becoming more complex with the identification of new and rarer genetic variants such as GANAB. Magnetic resonance imaging (MRI) and computed tomography (CT) continue to be the main imaging modalities used to evaluate ADPKD. Total kidney volume (TKV) continues to be the most validated and most used measure to assess disease progression. Since the publication of the previous consensus recommendations, the use of the Mayo Clinic Classification for prognostication purposes has been validated in patients with class 1 ADPKD. Recent evidence supports the benefits of a low-osmolar diet and dietary sodium restriction in patients with ADPKD. Evidence from the Replicating Evidence of Preserved Renal Function: an Investigation of Tolvaptan Safety and Efficacy in ADPKD (REPRISE) trial supports the use of ADH (antidiuretic hormone) receptor antagonism in patients with ADPKD 18 to 55 years of age with eGFR (estimated glomerular filtration rate) of 25 to 65 mL/min/1.73 m2 or 56 to 65 years of age with eGFR of 25 to 44 mL/min/1.73 m2 with historical evidence of a decline in eGFR >2.0 mL/min/1.73 m2/year. Limitations: Available literature was limited to English language publications and to publications indexed in PubMed, the Cochrane Library, and Google Scholar. Implications: Advances in the assessment of the risk of disease progression include the validation of the Mayo Clinic Classification for patients with class 1 ADPKD. Advances in the pharmacological management of ADPKD include the expansion of the use of ADH receptor antagonism in patients 18 to 55 years of age with eGFR of 25 to 65 mL/min/1.73 m2 or 56 to 65 years of age with eGFR of 25 to 44 mL/min/1.73 m2 with historical evidence of a decline in eGFR >2.0 mL/min/1.73 m2/year, as per the results of the REPRISE study.
A Community-Based Clinical Breast Examination Program: Is it Feasible?
Authors: Singh Ranjit K. Pritam , Abdullah Zainab , Saleh Salimah 
Published: October 2018

Purpose Breast cancer presents in advanced stages in low- and middle-income countries where screening mammography is not available. In Malaysia, almost 60% of women, particularly in those the low socioeconomic group, present with stage III and IV breast cancer. We evaluated the feasibility of a community-based early detection program using clinical breast examination (CBE) carried out by the Breast Cancer Welfare Association, a nongovernmental organization. Methods Using a mobile clinic that was equipped to carry out CBE, a team of six health professionals trained in CBE techniques reached out to urban poor communities on the outskirts of the city and the rural population at the invitation of community leaders. At these events, breast self-awareness was taught to the community, with particular regard to the signs and symptoms of breast cancer and to breast self-examination. CBE was carried out in those women who agreed to be examined. Women who were found to have breast lumps were referred to the nearest public hospital and were observed for compliance. Women with anxiety were counseled. Results In 2016 to 2017, CBE was performed for 7,503 women, and 295 (3.9%) were found to have a palpable breast lump. These women were referred to the nearest hospital for additional assessment. Of these women, 156 (52.9%) consulted the doctor and sought additional examination. A total of 153 patients had no malignant findings, whereas three were found to have breast cancer. The mean age for women found to have a palpable breast lump was younger than 40 years—35 years in 2016 and 33 years in 2017—whereas the median age among the 295 women was 33 years in 2016 and 31 years in 2017 (range, 17 to 67 years). Conclusion A community outreach program using CBE as a method of early detection is feasible in Malaysia. Additional study is required to determine why 47% of those who were found to have a breast lump did not seek additional assessment. AUTHORS' DISCLOSURES OF POTENTIAL CONFLICTS OF INTEREST The following represents disclosure information provided by authors of this manuscript. All relationships are considered compensated. Relationships are self-held unless noted. I = Immediate Family Member, Inst = My Institution. Relationships may not relate to the subject matter of this manuscript. For more information about ASCO's conflict of interest policy, please refer to www.asco.org/rwc or ascopubs.org/jco/site/ifc . No COIs from the authors.
Maternal High Triglyceride Levels During Early Pregnancy and Risk of Preterm Delivery: A Retrospective Cohort Study
Authors: Lin Xian-hua , Wu Dan-dan , Li Cheng , Xu Yi-jing , Gao Ling , Lass Geffen , Zhang Jian , Tian Shen , Ivanova Deyana , Tang Lin , Chen Lei , Ding Rong , Liu Xi-mei , Han Mi , Fan Jian-xia , Li Xiao-feng , Sheng Jian-zhong , O’Byrne Kevin T , Huang He-feng 
Published: October 2018

Abstract

Context
Maternal obesity increases the risk of preterm delivery. Obesity is known to be associated with altered lipid metabolism.

Objective
To investigate the associations between high maternal triglyceride (mTG) levels during early pregnancy and risks of preterm delivery stratified by early pregnancy body mass index (BMI).

Design
Retrospective cohort study.

Setting
University-based maternity center.

Patients
49,612 women with singleton pregnancy who underwent fasting serum lipid screening during early pregnancy.

Main Outcome Measures
Risk of preterm delivery (total, &lt;37 weeks; early, 28 to 33 weeks; and late, 34 to 36 weeks).

Results
Among women enrolled, 2494 had a preterm delivery, including 438 early preterm and 2056 late preterm delivery. High mTG (&gt;90th percentile, 2.04 mM) was associated with shortened gestation. Risks of total, early, and late preterm deliveries increased with mTG levels, and the high mTG–related risk was highest for early preterm delivery [adjusted odds ratio (AOR) 1.72; 95% CI, 1.30 to 2.29]. After stratification by BMI, high mTG was associated with risk of preterm delivery in both overweight or obese (OWO) women (AOR 1.32; 95% CI, 1.02 to 1.70) and women with normal BMI (AOR 1.36; 95% CI, 1.16 to 1.59). In additional sensitivity analyses, we found that high mTG was related to higher risks of preterm delivery among OWO women and women with normal BMI (AOR, 1.54; 95% CI, 1.07 to 2.22 and 1.62, 1.34 to 1.96, respectively), especially early preterm delivery (AOR 2.47; 95% CI, 1.19 to 5.10, and AOR 2.50; 95% CI, 1.65 to 3.78, respectively).

Conclusions
High mTG level during early pregnancy increased the risks of preterm delivery not only in OWO women but also in women with normal BMI.
Physicians in Print: Letters to the Editor and the Medicaid Expansion
Authors: Singer Phillip M. 
Published: October 2018

Abstract
The June 2012 Supreme Court decision on Medicaid expansion provides insight into how physicians and the general public engaged with the political and policy-making process. This study assesses the making of public statements on Medicaid policy, through analyzing letters to the editor published in one hundred newspapers across the United States. A total of 2,792 individual letters to the editor were coded for their stance, use of evidence, use of personal experience, and framing of the Medicaid expansion. Both the general public and physicians expressed support for expansion in their letters, but physicians were more likely to emphasize the role of Medicaid expansion at improving the quality of care and saving lives. Additionally, physicians were more likely to cite evidence and personal experiences while framing their position on Medicaid expansion. There is no evidence of a shift in stance on Medicaid expansion after the election of Donald Trump, and Medicaid policy changes made by the new administration highlight the importance of the public engaging in the political and policy-making process of Medicaid.
Ophthalmic signs in Ugandan adults with HIV-associated cryptococcal meningitis: A nested analysis of the ASTRO-CM cohort
Authors: Atherton Rachel R , Ellis Jayne , Cresswell Fiona V , Rhein Joshua , Boulware David R 
Published: October 2018

Cryptococcal meningitis is a leading cause of morbidity and mortality among HIV-infected persons, accounting for 15% of AIDS-related deaths. Visual disturbance is commonly reported, and a wide range of ophthalmic signs may be present on examination. There is limited published literature to date describing the range and incidence of ophthalmic signs in HIV-associated cryptococcal meningitis. Nested within the Adjunctive Sertraline for the Treatment of HIV-Associated Cryptococcal Meningitis (ASTRO-CM) trial (ClinicalTrials.gov number: NCT01802385), we conducted an observational study of 696 Ugandan adults with HIV-associated cryptococcal meningitis. Patients were screened for visual disturbance and external ophthalmic signs at initial presentation and at follow-up appointments over 18 weeks. Assessment comprised simple clinical history and basic examination and required no specialist equipment. More than a quarter of our cohort demonstrated ocular signs or symptoms, which were observed throughout the study period. A broad range of ocular signs were demonstrated: these included neurological signs (10.9%), localized ocular pathology (4.5%), and evidence of concurrent systemic disease (12.9%). The range of signs observed demonstrates the complexities of case management in patients with advanced HIV and cryptococcosis and also the importance of basic ocular examination in low resource settings. There remains an urgent need for studies conducting comprehensive ocular examination in patients with HIV-associated cryptococcal meningitis; these studies should include formal assessment of visual acuity, slit lamp examination and dilated indirect ophthalmoscopy. Prospective studies should investigate whether there is a correlation between reported visual disturbance and objective signs, in order to further clarify the underlying mechanisms and to guide effective diagnosis, follow-up and management.
Evolution versus entrenchment: Debating the impact of digitization, democratization and diffusion in the global fashion industry
Authors: Brydges Taylor , Hracs Brian J. , Lavanga Mariangela 
Published: October 2018

Abstract
In the report The State of Fashion 2017, written by Business of Fashion and the McKinsey Institute (2016), industry executives used three words to describe the current state of the fashion industry: uncertain, changing and challenging. Indeed, the fashion industry is undergoing dramatic transformations, from digitization and the rise of ‘see now, buy now’ fashions, to brands redefining the function and timing of fashion weeks, and increasing levels of global integration and competition (Crewe 2017). As such, the fashion industry has been recognized as a valuable lens through which to explore significant and ongoing changes to the production, curation and consumption of goods, services, and experiences (Brydges et al. 2014; Brydges 2017; D’Ovidio 2015; Hracs et al. 2013; Lavanga 2018; McRobbie 2016; Pratt et al. 2012).
Drawing inspiration from this stream of scholarship, we organized four sessions titled Trending Now: The Changing Geographies of Fashion in the Digital Age at the Royal Geographical Society and Institute of British Geographers (RGS-IBG) conference in London, 30 August – 1 September 2017. In these sessions, researchers and practitioners from a wide range of locations and disciplines – including fashion studies, media studies, cultural economics, business and geography – came together to share research related to the structures, labour dynamics, spaces, value propositions and practices of the contemporary fashion industry.
While a range of issues were discussed, the sessions were connected by an overarching theme. Namely, the extent to which power in the fashion industry is expanding or consolidating. While there is a prominent discourse that states that structures, systems and spaces within the global fashion industry have been (and will continue to be) disrupted by new actors, technologies, practices and cities, we collectively questioned whether the fashion industry has really entered an era of democratization, or if established power structures remain entrenched. Through empirical case studies from a variety of geographic contexts – from India to Italy – about different actors and activities within the industry, each presentation contributed new evidence and perspectives to this debate. The discussion below distils some of the key themes that emerged.