bluuebunny/crossref_metadata_2025_split huggingface dataset:
Water‐soluble vitamin content of sun‐dried Corinthian raisins (Vitis vinifera L., var. Apyrena)
Authors: Panagopoulou Eirini A , Chiou Antonia , Karathanos Vaios T 
Published: May 2019

AbstractBACKGROUNDCorinthian raisins are dried vine products, representing approximately 3% of the world dried vine fruit production. The majority of Corinthian raisin production is of Greek origin. Studies on the B‐group vitamin content of Corinthian raisins produced in Greece as well as on the effect of region and cultivation altitude on the B‐group vitamin content are absent in the literature.RESULTSCorinthian raisin vitamin content was evaluated by reversed‐phase high‐performance liquid chromatography after acid and enzymatic hydrolysis in terms of raisin subcategory, i.e. regions of cultivation, crop‐to‐crop variations, and cultivation altitude. Eight vitamers from five different vitamins were identified and quantified in Corinthian raisins. Vitamin B3 (0.77–2.82 g × 10−2 × kg−1) was found to predominate, followed by B6 (0.27–0.37 g × 10−2 × kg−1), B1 (0.19–0.22 g × 10−2 × kg−1), and B2 (0.10–0.15 g × 10−2 × kg−1). B9 content was up to 7.1 g × 10−5 × kg−1. Minor differences were observed among regions of cultivation, cultivation altitude and crop‐to‐crop variations.CONCLUSIONThis study revealed the presence of several water‐soluble vitamins in Corinthian raisins that, together with other health‐promoting micronutrients present in the product, further reinforce its place as part of a healthy diet. © 2019 Society of Chemical Industry
Preoperative neutrophil‐to‐lymphocyte ratio predicts malignancy and recurrence‐free survival of solid pseudopapillary tumor of the pancreas
Authors: Yang Feng , Bao Yun , Zhou Zhongwen , Jin Chen , Fu Deliang 
Published: May 2019

AbstractBackgroundSystemic inflammatory markers such as neutrophil‐to‐lymphocyte ratio (NLR) and platelet‐to‐lymphocyte ratio (PLR) have been shown to be prognostic for many types of pancreatic malignancy. The aim of this study was to evaluate the prognostic role of these markers in patients with solid pseudopapillary tumor of the pancreas (SPTP).MethodsPatients who underwent surgical resection for histologically confirmed SPTP were retrospectively reviewed in our institution. Preoperative NLR and PLR were calculated. Clinicopathologic data were correlated with the presence of malignant potential and recurrence‐free survival (RFS).ResultsA total of 113 patients with SPTP were included in this study. Of them, 23 were men and 90 were women, with a median age of 35 years (interquartile range, 25‐44). The optimal cut‐off values for malignant SPTP were 3.22 for NLR, and 75.5 for PLR, respectively. Univariate analysis showed that high NLR (>3.22) and white blood cell count more than 9.96 × 109/L were predictive of a malignant SPTP. Meanwhile, high NLR (P = 0.001) and age more than 35 years (P = 0.026) were associated with worse RFS. On multivariable analyses, high NLR was the only independent predictor of malignant SPTP (odd ratio 6.871; 95% confidence interval [CI], 1.482‐31.864; P = 0.014) and RFS (hazard ratio 12.633; 95% CI, 1.758‐90.790; P = 0.012).ConclusionsThis study highlights the supportive role of preoperative NLR in predicting malignancy and RFS of SPTP patients. Further studies including a larger cohort of patients are needed to corroborate our findings.
Changing odds of survival over time among patients undergoing surgical resection of extremity soft tissue sarcoma
Authors: Kim Yongsung , Kim Han‐Soo , Cho Hwan Seong , Han Ilkyu 
Published: May 2019

AbstractBackgroundWhile survival after surgical treatment of extremity soft tissue sarcoma (STS) is traditionally reported as actuarial survival, conditional survival (CS) may be more clinically relevant as it accounts for time already survived. We compared actuarial survival and CS of STS patients.Materials and MethodsWe analyzed 567 patients who underwent surgery for localized extremity STS. Actuarial survival was estimated using the Kaplan‐Meier method. Cox proportional hazards model was used to evaluate factors associated with disease‐specific survival. Five‐year CS (CS5) estimates at “χ” year(s) after surgery were calculated as CS5 = S(χ + 5)/S(χ).ResultsWhereas actuarial survival decreased over time, CS5 increased. The postsurgical 1‐, 3‐, and 5‐year CS5 values were 84.5%, 90.0%, and 93.8%, respectively, whereas the 6‐, 8‐, and 10‐year actuarial survival rates were 82.0%, 79.4%, and 78.5%, respectively. The calculated CS5 exceeded actuarial survival especially in patients with risk factors such as large tumor size and Federation Nationale des Centers de Lutte Contre le Cancer (FNCLCC) grades 2 and 3 tumors. Patients with tumor size ≥5 cm had an actuarial survival of 73.9% at 10 years compared to a CS5 of 95.4% in patients alive at 5 years. Likewise, patients with FNCLCC grade 3 tumors had an actuarial survival of 71.1% at 10 years compared to a CS5 of 96.0% in patients alive at 5 years.ConclusionsSurvival estimation by determination of CS can be dynamic and accurate especially in high‐risk patients. CS can be useful for survival prediction and clinical decision making in extremity STS patients.
Long‐term results of the Global Modular Replacement System tumor prosthesis for reconstruction after limb‐sparing bone resections in orthopedic oncologic conditions: Results from a national cohort
Authors: Yilmaz Müjgan , Sørensen Michala S. , Sæbye Casper , Baad‐Hansen Thomas , Petersen Michael M. 
Published: May 2019

AbstractBackground and ObjectivesWe investigated implant revision, implant failure, and amputation risk after limb‐sparing bone tumor surgery using the Global Modular Replacement System (GMRS) tumor prosthesis in patients suffering from bone sarcomas (BS), giant cell tumors (GCT), or metastatic bone disease (MBD).Material and MethodsA retrospective study of a nationwide consecutive cohort (n = 119, 47 [12‐81] years, M/F = 65/54) having limb‐sparing surgery and reconstruction using the GMRS tumor prosthesis due to bone tumors (BS/GCT/MBD = 70/8/41) from 2005 to 2013. Anatomical locations were as followed: distal femur (n = 49), proximal femur (n = 41), proximal tibia (n = 26), or total femur (n = 3). Kaplan‐Meier survival analysis and competing risk analysis with death as a competing risk were used for statistical analysis.ResultsFor BS and GCT patients, 5‐year patient survival was 72% (95% confidence interval [CI]: 59‐85%) and for MBD 33% (95% CI: 19‐48%). Thirty‐two patients underwent revision surgery (5‐year revision incidence 14%; 95% CI: 8‐21%). Twelve patients had revision of bone‐anchored parts (implant failure) with a 5‐year revision incidence 6% (95% CI: 2‐10%). Ten amputations were performed due to local relapse (n = 9) or recurrent infections (n = 1) with a 5‐year incidence of amputation: 8% (95% CI: 3‐13%).ConclusionsWe identified a low risk of revision and amputation when using the GMRS tumor prosthesis for limb‐sparing bone tumor.
Nine components pharmacokinetic study of rat plasma after oral administration raw and prepared Semen Cassiae in normal and acute liver injury rats
Authors: Yang Bing , Xie Li , Peng Siying , Sun Kanping , Jin Junjie , Zhen Yanping , Qin Kunming , Cai Baochang 
Published: May 2019

AbstractIn China, Semen Cassiae has long been used to protect liver, brighten eyes, and relieve constipation. Prepared Semen Cassiae is produced from raw Semen Cassiae by processing, the two forms of Semen Cassiae have different clinical applications. Pathological state is an important factor affecting the efficacy of drugs, the pharmacokinetic behavior of drugs could be significantly changed when people or animal were under different pathological state. To clarify the effect of processing mechanism and pathological state for pharmacokinetic behavior, the pharmacokinetics of nine components of raw and prepared Semen Cassiae under normal and acute liver injury rats were examined. The results showed that the bimodal phenomenon appeared on the plasma concentration‐time profiles of obtusin, emodin, chrysophanol, aloe emodin and rhein. The Tmax of aurantio‐obtusin, obtusin, chrysoobtusin, emodin, chrysophanol, aloe emodin, physcion in normal groups administrated prepared Semen Cassiae were shorter than those administrated raw Semen Cassiae. For the AUC0–t, aurantio‐obtusin, obtusin, chrysoobtusin, chrysophanol, aloe emodin and physcione in model groups administrated prepared Semen Cassiae were significantly higher than other groups, unlike above components, rhein had poor absorption in model groups. The study would be useful for further studies on pharmacokinetics and clinical application of raw and prepared Semen Cassiae.
Study of the peak broadening due to detection in the electrophoretic separation of DNA by CE and microchip CE and the application of image sensor for ultra‐small detection cell length
Authors: Ni Yi , Zhao Yubin , Chen Qinmiao , Yamaguchi Yoshinori , Dou Xiaoming 
Published: May 2019

AbstractNarrow peaks are important to high‐resolution and high‐speed separation of DNA fragments by capillary electrophoresis and microchip capillary electrophoresis. Detection cell length is one of the broadening factors, which is often ignored in experiments. However, is it always safe to neglect detection cell length under any condition? To answer this question, we investigated the influence of detection cell length by simulation and experiments. A parameter named as detection cell length ratio was proposed to directly compare the detection cell length and the spatial length of sample band. Electrophoretic peaks generated by various detection cell length ratios were analyzed. A simple rule to evaluate the peak broadening due to detection cell length was obtained. The current states of the detection cell length of detection system and their reliabilities in capillary electrophoresis and microchip capillary electrophoresis were analyzed. Microchip capillary electrophoresis detection with an ultra‐small detection cell length of 0.36 μm was easily achieved by using an image sensor.
Determination of major components from Radix Achyranthes bidentate using ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry and an evaluation of their anti‐osteoporosis effect in vitro
Authors: Tao Yi , Huang Surun , Yan Jizhong , Cai Baochang 
Published: May 2019

AbstractEcdysterone and saponins are the most characteristic components of Radix Achyranthes bidentate, which acts on the human body to promote collagen synthesis and stimulates cell growth. However, the relationship between these components and the differentiation of MC3T3‐E1 osteoblastic cells is unknown. We developed a rapid ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry method for direct determination of one ecdysterone and four saponins in crude and salt‐processed Radix Achyranthes bidentate. The method was interrogated in terms of linearity, intra‐ and inter‐day precision, repeatability, stability and recovery. The method was linear within the concentration ranges of 0.003–336 μg/mL for β‐ecdysterone, 0.0035–130 μg/mL for 25S‐inokosterone, 0.004–423 μg/mL for ginsenoside Ro, 0.0036–66 μg/mL for chikusetsusaponin IV and 0.0044–111 μg/mL for chikusetsusaponin IVa. The intra‐ and inter‐day precisions were all within 2.7%. The standard addition method determined recovery rates for each component (98.7–102.5%). The method was successfully applied to simultaneously quantify five components in ten batches of crude and salt‐processed Radix Achyranthes bidentate. Subsequently, the examination of these extracts on the differentiation of MC3T3‐E1 osteoblastic cells were carried out. Finally, the relationships between the contents of five components and their anti‐osteoporosis effect were investigated by using canonical correlation analysis.
Preparation of aptamer‐bound polyamine affinity monolithic column via a facile triazine‐bridged strategy and application to on‐column specific discrimination of ochratoxin A
Authors: Yu Xia , Lai Shuoke , Wang Li , Chen Yiqiong , Lin Xucong , Xie Zenghong 
Published: May 2019

AbstractDeveloping a high‐performance modification protocol is critical for efficiently fabricating affinity monolith. Herein, by using 2,4,6‐trichloro‐1,3,5‐triazine as the linker, a simple triazine‐bridged approach was proposed for efficiently fabricating aptamer‐grafted polyhedral oligomeric silsesquioxane‐polyethyleneimine affinity monolith with high specificity toward ochratoxin A. Experimental parameters, column characteristics and specificity performances of the resultant affinity monolith were investigated in detail. Under the optimal conditions, 2,4,6‐trichloro‐1,3,5‐triazine was rapidly grafted on the polyamine matrix, and effectively applied to the subsequent bridge linkage of aptamers. It was simple and effective, which resulted in a significant decrease of modification time, excellent properties including the high coverage density of aptamer up to 1799 pmol/μL and sensitive detection of ochratoxin A as low as 10 pg/mL in beer samples. This protocol provides a facile approach for fabricating aptamer‐grafted polyamine affinity monoliths with highly selective discrimination performance.
A model study on the mechanism and kinetics for the dissociation of water anion
Authors: Huyen Trinh Le , Duong Long Van , Nguyen Minh Tho , Lin Ming Chang 
Published: May 2019

AbstractQuantum chemical computations using both density functional theory (B3LYP functional) and wavefunction (MP2 and CCSD(T)) methods, with the 6‐311++G(3df,2p) and aug‐cc‐pVnZ (n = D,T,Q) basis sets, in conjunction with a polarizable continuum model (PCM) method for treating structures in solution, were carried out to look again at a series of small negatively charged water species [(H2O)n]•–. For each size n of [(H2O)n]•– in aqueous solution with n = 2, 3, and 4, two distinct structural motifs can be identified: a classical water radical anion formed by hydrogen bonds and a molecular pincer in which the excess electron is directly interacting with H atoms. In aqueous solution, both motifs have comparable energy content and likely coexist and compete for the ground state. Some water anion isomers can dissociate when interaction with a water molecule, [(H2O)n]•– + H2O → H•(H2O)m + OH–(H2O)n–m, through successive hydrogen transfers with moderate energy barriers. This reaction can also be regarded as a water‐splitting process in which the H transfers involved take place mainly within a water trimer, whereas other water molecules tend to stabilize transition structures through microsolvation rather than direct participation. Calculated absolute rate constants for the reversed reaction H•(H2O)2 + OH–(H2O)2 → [(H2O)4]•־ + H2O with both H and D isotopes agree well with the experimentally evaluated counterpart and lend a kinetic support for the involvement of a tetramer unit.
Impact of AASM 2012 Recommended Hypopnea Criteria on Surgical Outcomes for Obstructive Sleep Apnea
Authors: Jung Su Young , Rhee Eui Hyeok , Al‐Dilaijan Khalid Fawzi , Kim Sung Wan , Min Jin‐Young 
Published: May 2019

ObjectivesTo assess the impact of the American Academy of Sleep Medicine (AASM)
2012Rec criteria on the surgical success rate and polysomnography (PSG) parameters of patients with obstructive sleep apnea (OSA).Study DesignA prospective clinical trial.MethodsA total of 60 patients who performed surgery for treatment of OSA at the sleep breathing disorder center of a tertiary referral university hospital from October 1, 2015, to September 30, 2016, were enrolled. Preoperative and postoperative PSG were performed and scored using AASM
2007Rec and AASM
2012Rec. Surgical success was determined by criteria 1 (postoperative Apnea‐Hypopnea Index [AHI] <20% and 50% reduction) and criteria 2 (postoperative AHI <10), and surgical success rates according to AASM
2007Rec and AASM
2012Rec were compared.ResultsPSG parameters including AHI, hypopnea index, respiratory effort‐related arousals, respiratory disturbance index, and supine AHI scored significantly higher with AASM
2012Rec compared to AASM
2007Rec criteria, both preoperatively and postoperatively (all P < 0.05). Furthermore, when compared to the AASM
2007Rec criteria, AASM
2012Rec resulted in increased proportion of patients with severe OSA (70.0% vs. 95.0%; P < 0.001). For the surgical outcomes, AASM
2012Rec showed significantly lower surgical success rate both in criteria 1 (51.7% vs. 38.3%) and criteria 2 (35.0% vs. 18.3%) compared to the AASM
2007Rec, respectively (P < 0 .05).ConclusionsThis study demonstrated that AASM
2012Rec tends to decrease the success rate of surgical outcome for OSA patients, suggesting that defining success of surgical treatment for OSA could be changed dramatically according to the hypopnea scoring criteria.Level of Evidence4Laryngoscope, 130:825–831, 2020
Drug‐Induced Sleep Endoscopy Upper Airway Collapse Patterns and Maxillomandibular Advancement
Authors: Kastoer Chloé , Op de Beeck Sara , Dom Marc , Neirinckx Thérèse , Verbraecken Johan , Braem Marc J. , Van de Heyning Paul H. , Nadjmi Nasser , Vanderveken Olivier M. 
Published: May 2019

Objectives/HypothesisTo evaluate upper airway (UA) collapse patterns during drug‐induced sleep endoscopy (DISE) and maxillomandibular advancement (MMA) surgery outcome, paying particular attention to the DISE phenotype complete concentric collapse at the level of the palate (CCCp).Study DesignProspective case series.MethodsNineteen patients diagnosed with obstructive sleep apnea (OSA) prospectively underwent mandibular advancement device (MAD) treatment followed by MMA surgery. UA collapse patterns were evaluated before and after MMA surgery. Baseline apnea‐hypopnea index (AHI) was compared with AHI during MAD treatment and after MMA surgery. UA collapse patterns and the presence of baseline CCCp were assessed. AHI differences between patients with and without CCCp after MMA surgery were compared.ResultsIn 14 patients, a full dataset was obtained: 8/6 (male/female), mean age = 51 ± 7 years, body mass index (BMI) = 25.6 ± 3.7 kg/m2, and baseline AHI = 40.2 ± 25.6 per hour of sleep. MMA surgery reduced AHI to 9.9 ± 7.2 per hour of sleep (P = .0001). Eight patients benefited from additional AHI reduction compared to MAD treatment (P = .0113). Six out of 14 (43%) patients showed baseline CCCp. Baseline patient characteristics (age, BMI, and AHI) did not significantly differ between patients with and without CCCp. AHI was equally reduced after MMA, whether (P = .0145) or not (P = .0075) CCCp was present at baseline. All patients showed resolution of CCCp (P = .0159) during postoperative DISE.ConclusionsCCCp seems not to be a negative predictor for MMA surgery outcome measured by AHI reduction. Furthermore, MMA is likely to eliminate CCCp. Therefore, MMA might be a solution for OSA patients showing CCCp and broadens the perspective for personalized medicine and combination therapy. However, a large sample is required for definitive results.Level of Evidence4 Laryngoscope, 130:E268–E274, 2020
Infectivity of murine papillomavirus in the surgical byproducts of treated tail warts
Authors: Best Simon R. , Esquivel Daniel , Mellinger‐Pilgrim Rebecca , Roden Richard B. S. , Pitman Michael J. 
Published: May 2019

Objectives/HypothesisHuman papillomavirus (HPV) is a highly stable DNA virus that causes disease in human organ systems, including the larynx and oropharynx. The treatment of HPV‐associated diseases with scalpels, lasers, and other surgical instruments has the potential to release infectious particles, placing healthcare workers at risk. The objectives of this study were to create a reproducible in vivo animal model of papillomavirus infectivity and to compare the infectivity of byproducts of surgically treated mouse papillomavirus (MmuPV1) warts.Study DesignAnimal study.MethodsNude laboratory mice (Mus musculus) with established MmuPV1 tail warts were treated with scalpel excision, potassium titanyl phosphate (KTP) laser ablation, and coblator treatment. Uninfected nude mice were challenged with surgical byproducts, including ablated and heated tissue, and surgical smoke products. The incidence and time course of the appearance of warts was recorded.ResultsThere was rapid transmission of virus in mice challenged with scalpel‐treated warts, with 50% penetrance of infection at day 13 and 100% at day 32. For KTP‐treated warts, there was the slower development of infection (50% by day 35) but 100% penetrance by day 52. Coblator‐treated tissue reached 50% penetrance at day 59 and a maximum of 73% penetrance. Smoke plume captured during treatment with the KTP laser and coblator was highly infectious, as was the material captured in a laser filter.ConclusionsMmuPV1 remains infectious in all modes of surgically treated tissue, and the smoke plume is capable of transmitting infection. Healthcare workers should use appropriate precautions to lower their risk of infection when treating papillomavirus‐associated diseases.Level of EvidenceNALaryngoscope, 130:712–717, 2020
Preventing Restenosis of Marsupialized Rathke Cleft Cysts Using a Nasoseptal Flap Lining
Authors: Kuan Edward C. , Trent Monica S. , Luu Neil N. , Kohanski Michael A. , Tong Charles C. , O'Malley Bert W. , Adappa Nithin D. , Palmer James N. 
Published: May 2019

ObjectivesRathke cleft cysts (RCCs) are rare, benign lesions formed from remnants of Rathke pouch during embryologic development. However, following marsupialization, maintaining tract patency remains a challenge. Several techniques for decreasing stenosis have been described, including free mucosal grafting, silastic spacing, and using steroid‐eluting stents. Nasoseptal flaps (NSFs) have a reliable vascular supply and are widely utilized in skull base reconstruction. We present a novel technique to maintain patency of the marsupialized RCC cavity by lining it with an NSF to promote long‐term drainage and re‐epithelialization of the RCC cavity.MethodsRetrospective chart review of all patients who underwent surgery for symptomatic RCCs. These patients underwent endoscopic endonasal surgery between February 3, 2016, and June 12, 2018, with marsupialization followed by circumferential NSF lining. Primary outcomes include symptomatic control, surgical complications, and RCC cavity patency.ResultsSeven patients underwent RCC marsupialization with no intraoperative cerebrospinal fluid leak, followed by circumferential lining of the marsupialized cavity with an NSF. In each case, no cyst re‐accumulation or recurrent symptoms were noted, and the NSF lining provided long‐term patency of the tract in all cases to a mean of 6.7 ± 10.1 months of follow‐up, including one patient with sustained patency at 29 months follow‐up.ConclusionUtilization of the NSF to “stent open” the RCC cavity following marsupialization is a safe and effective means to prevent restenosis. Advantages include sustained patency of RCC cavity for complete drainage and a dependable vascular supply.Level of Evidence4Laryngoscope, 129:2258–2261, 2019