bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of the microbial communities and treatment units in a waste stabilization pond system treating wastewater from an industrial complex located in Northeastern Brazil
Authors: Júnior Luiz Pereira Silva , Silva Bruna Kelly de Oliveira , Santos Nathália Bandeira Carvalho dos , de Araújo Natercia Correa , Amaral Fernanda Magalhães , Júnior Bartholomeu Siqueira , de Oliveira Fábio Henrique Portella Corrêa , Bortoleti Kyria Cilene de Andrade , Motteran Fabrício , Fernandes Bruna Soares , Vidal Ana Christina Brasileiro , Júnior Marcos Antônio de Morais , Gavazza Savia 
Published: November 2024

Abstract

The Multifactory Effluent Treatment Plant (ETE-MF), located within an industrial complex in Recife, Brazil, receives effluents from 15 industries from different sectors, as well as domestic wastewater. This plant is recognized as efficient by the state sanitation company. This raises the question of what are the causes of this scenario and whether this process could be replicated. In this sense, this research aimed to reveal the physicochemical aspects of the MF-WWTP and the microbial community that has established itself there during decades of operation. Overall, the results showed COD (Chemical Oxygen Demand) removal of 84.0% and COD/BOD (BOD - Biological Oxygen Demand) ratios of 1.78 and 5.79 for raw and filtered untreated effluents, respectively. The microbial community analysis showed that bacteria belonging to the Fervidobacterium genus was the most abundant in all sampled points in all ponds, with an average relative abundance of 64%. This organism belongs to the Thermotogaceae family, widely distributed in nature and frequently found in salt flats, oil and petroleum wells, refineries and biodigesters and seems to be crucial for the performance of the treatment plant. This polishing process was completed by the microalgae community found mainly in pond 3, where the community was mainly composed of the genera Cyclotella and Planktothrix. The results provided scientific evidence for the technical analysis that the MF-WWTP has bioremediation potential and indicates that this efficiency is due to the microbial composition of the system.
Comprehensive Prognostic and Immunoinfiltration Analysis in Esophageal Squamous Cell Carcinoma Patients Using a Combined EMT and Ferroptosis-Related Biomarker Signature
Authors: Wang Zhidong , Gong Cheng , Chao Ce , Zhang Youpu , Qian Yiongxiang , Wang Bin , Wang Min , Liu Yang 
Published: November 2024

Abstract

Background
Limited research has been conducted on the interaction between ferroptosis and epithelial-mesenchymal transition (EMT) and their combined effect on esophageal squamous cell carcinoma (ESCC) patient prognosis. The present study aimed to develop a prognostic model based on the impact of ferroptosis and EMT on ESCC prognosis for clinical application.
Methods
Gene expression levels and clinical data of ESCC patients were obtained from the GSE53625 dataset in the Gene Expression Omnibus (GEO) database, and the data from the Cancer Genome Atlas (TCGA) were obtained as a validation set. By combining the results of Cox regression analysis and least absolute shrinkage and selection operator regression (LASSO) analysis, we selected nine genes associated with prognosis, which were then used to construct a prognostic model. Immune cell infiltration was evaluated using CIBERSORT and single-sample Gene Set Enrichment Analysis methods. Finally, in vitro experiments were conducted to assess the oncogenic effects of ACSL3 and VIM.
Results
Nine key genes were screened to construct ferroptosis and EMT integrated score (FEIS). Compared to the low-FEIS group, the high-FEIS group demonstrated shorter overall survival period. The immune infiltration analysis showed an increase in immune cell infiltration and elevated expression levels of immune checkpoint molecules in the high-FEIS group. A nomogram was constructed to accurately predict patient prognosis. Additionally, our in vitro experiments confirmed the oncogenic effects of ACSL3 and VIM.
Conclusion
Our study introduced a novel prognostic tool that integrates ferroptosis -and EMT-related biomarker, and offered valuable insights for developing personalized treatment strategies for ESCC patients.
Genomics on FHIR – A Feasibility Study of an interoperable genomics dataset to support the German National Strategy for Genomic Medicine
Authors: Haffer Nina , Stellmach Caroline , Sass Julian , Muzoora Michael R , Graefe Adam SL , Thun Sylvia , Vorisek Carina N 
Published: November 2024

Abstract

The German National Strategy for Genomic Medicine (genomDE) project aims to establish a nation-wide platform for medical genome sequencing to integrate genome sequencing into standard health care. Connecting information of genomics data generated by research and healthcare remains challenging due to the complexity of data and lack of standardization. Fast Healthcare Interoperability Resources® (FHIR)® is a standard for exchanging healthcare information electronically that has gained worldwide adoption within healthcare systems. This study analyzed how a dataset defined by stakeholders in genomDE could be mapped established international standards: the Genomics Reporting FHIR implementation guide (IG) version 2.0.0 that was created by Health Level Seven (HL7)’s Clinical Genomics working group, the Global Alliance for Genomics and Health (GA4GH)’s Phenopacket Schema, as well as the German Medical Informatics Initiative’s (MII) molecular genomics report IG. In addition, sample FHIR bundles and necessary search queries were created and validated. We concluded that most dataset items defined by the genomDE projects could be represented in FHIR, using preexisting FHIR profiles. For dataset elements for which no prior FHIR mapping existed, we could show that it was possible to extend the existing specifications through profiling of additional FHIR resources and the use of extensions. This study highlights that the identification and use of preexisting FHIR specifications increases interoperability and builds efficiencies in connecting genomics data models on an international level while considering national requirements.
Osteosynthesis with allograft screws in pediatric orthopedics: preliminary results
Authors: Gutierrez-Pereira Javier , Santos Flavio Paulos dos , Gutierrez-Carbonell Pedro , Garcia-Lopez Antonio 
Published: November 2024

Abstract

Background
A significant percentage of patients develop complications related to the use of implants made of stainless steel or titanium. The purpose of our study is to retrospectively analyze the clinical and radiological outcomes of allograft screws implanted in the Pediatric Orthopedic unit of our center.
Materials and Methods
A retrospective analysis was conducted, including 30 patients (16 females, 14 males) with lower limb pathologies who received 50 human cortical bone allograft screws. The study period covered 24 months, from January 1, 2020, to January 1, 2023. The average age was 12 years 7 months (range 3, 15 years). The mean follow-up was 14 months (range 8–24).
Results
After an average follow-up of 14 months, no patients developed minor or major complications, delayed union, or infections. Three patients reported discomfort due to implant prominence, and two of them (7%) required reoperation for screw head remodeling. The average resorption time was 9 months (range 5, 15 months). The postoperative AOFAS score increased by 38.28 points (statistical study) compared to the preoperative score.
Conclusion
Cortical bone allograft screws are an effective and safe osteosynthesis method for lower limb pathologies in pediatric orthopedics. It is important to avoid implant prominences that may cause discomfort to the patient and potentially require reoperation.
Bronchoalveolar Lavage Proteomics in Acute Exacerbation of Bronchiectasis
Authors: Lee Ju Yeon , Yang Jiyoul , Kim Jin Young , Do Yeji , Kim Min-Sik , Kye Dong Eun , Min Geonhui , Jeon In-Sook , Kim Eung-Gook , Choi Joong Kook , Lee Hyun , Yang Bumhee 
Published: November 2024

Abstract

Background: The molecular pathophysiology underlying the development of bronchiectasis with acute exacerbation at the proteomic level has not been clarified using bronchoalveolar lavage fluid samples. This study aimed to evaluate the bronchoalveolar lavage fluid inflammatory profiles associated with acute exacerbation of bronchiectasis.
Methods: We analyzed the bronchoalveolar lavage fluid specimens from 4 patients in the acute exacerbation status and 4 patients in a stable status using liquid chromatography-tandem mass spectrometry.
Results: A total of 1,577 proteins were identified using proteomic analysis, with 127 differentially expressed proteins. Of 127 differentially expressed proteins, 23 proteins showed more than 2-fold differences between the acute exacerbation and stable status groups. The acute exacerbation status was associated with 18 upregulated proteins (TPI1, CRP, BPI, ORM1, PTPRE, S100A9, BPY2, TPM4, ERVFC1-1, CYS1, CLEC3B, S100A8, PSAT1, NDUFA10, MDGA1, SPRR3, ALDOA, and PSMB2)and five downregulated proteins (MUC5B, HSPE1, KLK13, IGHA1, and MUC5AC). Pathway analysis revealed that the neutrophil degranulation pathway (R-HSA-6798695) was the most enriched pathway in these proteins, followed by the C-type lectin receptor pathway (R-HSA-5621481).
Conclusion: The bronchoalveolar lavage fluid protein expression in patients in the acute exacerbation status of bronchiectasis was significantly different from that in patients in the stable status, indicating that neutrophil degranulation and C-type lectin receptor pathways are the most enriched pathways during acute exacerbation.
Evaluation of Kosmic and Expectation-Maximization algorithms and different data pre-processing approaches to determine Upper Reference Limits for Insulin in adolescents
Authors: Freire Monica Di Calafiori , Dias Paulo Roberto Telles Pires , Arcuri Leonardo Javier , Souza Thiago Silva Pereira , Hirose Caio Kenji , Araujo Paula Bruna Mattos Coelho , Neves Mario Fritsch Toros 
Published: November 2024

Abstract

Background
The International Federation of Clinical Chemistry considers the a priori direct method the gold standard to laboratory reference interval (RI) determinations. However, this can be significantly challenging in the real world. Indirect methods may be more realistic tools to determine RI specific to local populations. More recently developed Kolmogorov-Smirnov distance and Expectation-Maximization mixture model separation methods are free from the implicit subjectivity of the earliest graphic indirect methods available. Objective: To evaluate the performance to establish upper reference limit (URL) for insulin in Brazilian adolescents, from 12 to 17 years old, by 2 indirect algorithms and 3 data pre-processing approaches, compared to a directly obtained URL.
Methods: This study was approved by the Ethics Committee of the Pedro Ernesto University Hospital, under the document number: 2.970.023. Data set 1 (DS1), used to obtain URL through direct method, consisted of individuals from ERICA Study, after exclusion of “not healthy” individuals according to available data. Data set 2 (DS2) also consisted of individuals from the Erica Study without application of any exclusion criteria, was submitted to Kolmogorov-Smirnov (Kosmic) and Expectation-Maximization (EM) algorithms, in 3 pre-processing settings. URL were compared through bias ratio (BR).
Results: DS1 and DS2 consisted of 14835 and 37892 individuals, 57.7% (n = 8559) and 60% (n = 22729) were female, respectively. Distribution was not normal in both data sets. URL by Kosmic and EM without data pre-processing were lower than the direct URL for all partitions. On log-transformed DB2 indirect calculations, Kosmic method URL was lower than the DB1 direct ones, except in one partition. EM and direct URLs were similar. When DB2 was submitted to Cox-Cox transformation, indirect URL were lower than the direct ones. EM BR results fitted below 0.375 in all partitions when data were log-transformed. Kosmic BR results were not consistently below the BR threshold.
Conclusion: EM algorithm determined FBI URL after log-transformation outperformed the Kosmic method and other pre-processing approaches, when compared to gold-standard URL. These findings can be explained by the right skewness of the FBI distribution.
In utero impacts of airway exposure to silver nanoparticles on male reproductive function in two generations of mice
Authors: Yokota Satoshi , Kawakami Tsuyoshi , Yoshioka Hiroki , Moriya Nozomu , Sekine Nao , Miura Nobuhiko , Kitajima Satoshi , Ichinose Takamichi , Oshio Shigeru 
Published: November 2024

Abstract

Considering the transgenerational inheritance of epigenetic information and the negative effect of nanoparticle exposure on male fertility, this study investigated the in utero effects of exposure of airway to silver nanoparticles (AgNPs) on male reproductive function. Exposure of pregnant mice to AgNPs (low dose: 5 µg/injection; high dose: 50 µg/injection) at embryonic day 7.5 and 14.5, by intratracheal administration, resulted in dose-dependent exacerbation of sperm parameters in both F1 and F2 males. Microarray analysis revealed significant upregulation of four microRNAs (miRNAs) and downregulation of seven miRNAs in the testes of F1 males in the high-dose group compared to those in the control group, and these results were further verified using quantitative real-time PCR. Interestingly, in the mature sperm as well, the high-dose group displayed significant upregulation of one and downregulation of three of the miRNAs dysregulated in the testes. Notably, in silico analyses predicted that the genes targeted by the differentially expressed miRNAs in the sperm were significantly associated with neuronal development. Neuronal migration in the cerebral cortex was dysregulated in the F2 males and was accompanied by depression-like behavior. This is the first report of prenatal exposure to AgNPs inducing germ cell development dysfunction in F1 males, which is involved in developmental toxicity in the F2 males born to these F1 males.
Depression as a Risk Factor for Gynecological Cancers: Evidence from a National Study
Authors: Wang Chaoyi , Xu Jin , Li Xiaodan , Jiang Lijuan 
Published: November 2024

Abstract

Background: Depression is one of the leading sources of disease burden globally and plays a significant role in the occurrence and development of many cancers, representing an important health risk. However, the relationship between depression and the risk of gynecologic cancers has not been fully assessed.
Objective: This study aims to explore the association between depression and the risk of gynecologic cancers.
Methods: The study selected 11,574 participants from the NHANES 2009-2018 cycles, among which 274 had gynecologic cancer (GC), 137 had cervical cancer (CC), 48 had ovarian cancer (OC), and 89 had endometrial cancer (EC). Box plots were used to assess the differences in PHQ-9 depression scores between cancer and non-cancer groups. Logistic regression models and restricted cubic spline (RCS) models were employed to evaluate the relationship between PHQ-9 scores and gynecologic cancers. Subgroup analyses and interaction tests examined the consistency of the association across different characteristics.
Results: There was a significant difference in PHQ-9 scores between the cancer group and the non-cancer group. In the multivariable logistic regression analysis, PHQ-9 scores were positively correlated with GC, OC, and EC, while no significant association was found with CC risk. The RCS model also indicated no nonlinear association. Additionally, subgroup analyses suggested that the relationship between PHQ-9 scores and CC and OC was consistent across groups, whereas the association between PHQ-9 scores and GC and EC showed heterogeneity in relation to race and marital status.
Conclusion: Depression is positively correlated with gynecologic cancers. Specifically, higher levels of depression are associated with an increased risk of OC and EC, while no significant association was found with CC risk. Future attention should be given to the impact of depression on the incidence of gynecologic cancers, particularly OC and EC.
Impact of ACE on Emotional Regulation and Future Time Perspectives in Borderline Personality Disorder: Mediating and Moderating Roles of Parental Involvement
Authors: Nayar Sonakshi , George Angeline Miriam , K Suvarna Jyothi 
Published: November 2024

Abstract

Background: Adverse Childhood Experiences (ACEs) are known to have significant psychological and physiological impacts on various aspects of adult life. This study aimed to examine the influence of ACEs on Emotional Regulation (ER) and Future Time Perspective (FTP) in young adults diagnosed with Borderline Personality Disorder (BPD) within an Indian context.
Method: The study explored the mediating and moderating roles of Parental Involvement in these relationships. A sample of 40 young adults, both male and female, from the Inpatient and Outpatient Departments of Psychiatry and Clinical Psychology from SRMC hospital, diagnosed with BPD, were assessed.
Result: The findings challenge established theoretical frameworks by showing that whereas ACEs strongly predict PI, neither ACEs nor PI were significant predictors of ER or FTP in this group. These results imply that the cultural setting, particularly the collectivist character of Indian society, may be a significant moderator of the psychological effects of early adversity in borderline personality disorder.
Conclusion: The paper also identifies other constraints that might have affected the results, such as a small sample size, gender imbalance, and cultural heterogeneity. Given the diversity in BPD symptomatology and the impact of culture, the study emphasizes the need for more research and interventions that are specifically suited to cultural differences.
MRI texture analysis of the lateral pterygoid muscle in patients with unilateral anterior disc displacement of the temporomandibular joint
Authors: Huang Tao , Zhao Shu-Fan , Song Zhi-Qiang , Gong Zhong-Cheng 
Published: November 2024

Abstract

Background
This study aimed to investigate the texture changes in the lateral pterygoid muscle (LPM) in patients with unilateral anterior disc displacement (uADD) of the temporomandibular joint with magnetic resonance imaging (MRI).
Methods
This retrospective study aimed to comparatively analyze the texture of the LPM in 232 patients with uADD from January 2022 to December 2023. Four groups were included in this study: the healthy joints of patients with uADD (H-TMJ), joints with anterior disc displacement (ADD) with reduction (ADDwR), joints with ADD without reduction (ADDwoR), and the joints of normal volunteers as the healthy group (HG). Five texture parameters were used for analysis: the angular second moment (ASM), contrast, inverse difference moment (IDM) and entropy.
Results
The average differences in the ASM, contrast, correlation, IDM, and entropy of the LPM between the joints of healthy individuals and those of individuals with uADD were − 1.16×10− 4, 7.65, 2.66×10− 5, 1.06×10− 2, and 9.21×10− 2, respectively, with P values less than 0.05, indicating statistical significance. The entropy values of the ADDwoR and ADDwR groups was 6.38×10− 2, with a statistically significant difference (P < 0.05). In addition, the H values for ASM, contrast, correlation, IDM, and entropy among the different degrees of disc displacement were 6.52, 15.45, 12.96, 7.72, and 11.66, respectively, with P < 0.05, indicating statistical significance. Through correlation analysis, the correlation coefficient between the disc displacement and the ASM was 0.082 (P > 0.05), with no statistical significance. The corresponding correlation coefficients for contrast, correlation, IDM, and entropy values were − 0.146, 0.102, 0.098, and − 0.092, respectively, with P < 0.05.
Conclusions
Patients with ADD experience changes in the texture parameters of the LPM; among these, the entropy value of the LPM has high value in distinguishing ADDwoR from ADDwR, which can be used as an indicator to evaluate diseases that cause changes in the lateral pterygoid muscles. In addition, the degree of disc displacement is correlated to different degrees to the texture parameters. However, more research is needed to confirm the correlation between each numerical texture change and the corresponding pathological changes in the LMP.
Myocardial flow reserve in chronic kidney disease and renal transplantation: A cross-sectional study of ammonia PET
Authors: NAKAO RISAKO , Nagao Michinobu , Nakao Masashi , Yamamoto Atsushi , Ando Kiyoe , Sakai Akiko , Yamaguchi Junichi 
Published: November 2024

Abstract

Objective Renal transplantation (RT)’s effects on coronary artery and microvascular diseases is not yet elucidated. We aimed to quantify myocardial blood flow (MBF) using 13N-ammonia positron emission tomography (NH3-PET) and compare the results between RT recipients and patients with chronic kidney disease (CKD).Methods Adenosine-stress NH3-PET measurements of 29 RT recipients and 564 patients with CKD were assessed. Stress and rest MBF were calculated from the dynamic data of the initial 2 min after the ammonia injection. Myocardial flow reserve (MFR) was defined as stress MBF divided by rest MBF; MFR < 2.0 or stress MBF < 2.0 ml/g/min was considered a significant decrease.Results Stress MBF for RT recipients and patients with end-stage and mild-stage CKD was 2.03 ± 0.60, 1.72 ± 0.62, and 2.07 ± 0.61 ml/g/min, respectively; their MFRs were 2.00 ± 0.60, 1.68 ± 0.51, and 2.26 ± 0.69, respectively. Stress MBF and MFR were significantly greater in RT recipients than in patients with end-stage CKD (P < 0.01). The MFR was significantly greater in patients with mild-stage CKD than in RT recipients (p < 0.05); however, there was no significant difference in stress MBF. The proportion of patients with MFR ≥ 2.0 or stress MBF ≥ 2.0 ml/g/min was significantly higher in RT recipients than in patients with end-stage CKD (51.7% vs. 26.3%, and 58.6% vs. 26.3%, p < 0.01).Conclusion RT may improve MFR in patients with end-stage CKD by acting on adenosine vasodilation.
Development of New Affinity Material for Urease Purification: Synthesis and Characterization
Authors: Uygun Hilmiye Deniz Ertuğrul , Coşkun Elif Esen , Öztürk Simge , Demir Münire Nalan 
Published: November 2024

Abstract

Urease (urea amidohydrolase, E.C.3.5.1.5) is an enzyme that catalyzes the hydrolyzation of urea to form ammonium and carbon dioxide. This is an essential reaction in industrial and medical applications. Urease has a vital role in accelerating the hydrolysis of the urea in the agricultural gut, calculating the amount of urea in biological fluids, urine removal from artificial kidneys, and urea removal in wastewater and fruit juices. Purifying urease is also crucial for treating some diseases, such as gastrointestinal infection and hypertension. This research aimed at developing an environmentally friendly green biocomposite with high mechanical stability by suspension polymerization of chitosan (CTS), 2-hydroxyethyl methacrylate (HEMA), and N-methacryloyl- (L) -glutamic acid (MAGA) and to determine its optimum conditions for urease purification. The synthesized p (HEMA-MAGA) -CTS sorbent was investigated by FTIR, XPS, XRD, TGA, SEM, and particle size analysis. After the characterization, the parameters affecting the adsorption of urease, such as pH, sorbent amount, contact time, initial urease concentration, heat, and ionic strength, were optimized. In this study, equilibrium adsorption isotherms of urease on the synthesized affinity sorbent were examined and The Freundlich isotherm was found to provide a much better fit than the Langmuir and Dubinin-Radushkevich isotherms. The urease adsorbed on the p(HEMA-MAGA)-CTS affinity sorbent was desorbed in over 75% yield with 1.0 M NaSCN (pH 8.0) in 2 hours. The maximum adsorption capacity was found to be 9.47 mg mg-1 at pH 4.0 and 25°C. p(HEMA-MAGA)-CTS for urease purification was successfully developed.