bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between slow wave sleep and blood pressure in insomnia
Authors: Ren Rong , Zhang Ye , Feng Xujun , Shi Yuan , Nie Yuru , Wang Yongming , Somers Virend K , Covassin Naima , Tang Xiangdong 
Published: November 2024

Abstract

Study Objectives
The majority of patients with insomnia exhibit abnormal sleep in objective testing (e.g. decreased sleep duration, decreased slow wave sleep [SWS]). Previous studies have suggested that some of these objective measures of poor sleep, such as decreased sleep duration, are associated with a higher risk of hypertension in insomnia. We examined the relationship between SWS and morning and evening blood pressure (BP) levels in patients with clinically diagnosed insomnia.

Methods
A total of 229 normal sleepers and 1378 insomnia patients were included in this study. Insomnia was defined based on standard diagnostic criteria with symptoms lasting ≥6 months. All participants underwent in-laboratory polysomnography. Patients were classified into quartiles of percent SWS. Evening and morning hypertension were defined using BP measurements taken in the evening before and in the morning after polysomnography, respectively. Multivariable logistic regression models were used to assess the relationship between insomnia, SWS, and hypertension.

Results
Insomniacs with &lt;3.5% SWS (OR 3.27, 95% confidence intervals [CI]: 1.31 to 7.66) and those with 3.5%–10.2% SWS (OR 2.38, 95% CI: 1.28 to 5.91) had significantly greater odds of morning hypertension compared to normal sleepers. No associations were seen in insomnia with 10.2%–15.8% SWS and with &gt;15.8% SWS. Significant effect modifications by sex (p = .043) were found, as decreased SWS was associated with morning hypertension only in men. Odds of evening hypertension were not significantly associated with SWS.

Conclusions
Decreased SWS is associated with morning hypertension in a dose-dependent manner in insomnia, especially in men.
Enhanced delta-gamma phase-amplitude coupling during phasic rapid eye movement sleep in isolated rapid eye movement sleep behavior disorder
Authors: Noh Tae-Gon , Choi Kang-Min , Jun Jin-Sun , Shin Jung-Won , Byun Jeong-Ick , Sunwoo Jun-Sang , Jung Ki-Young 
Published: November 2024

Abstract

Study Objectives
This study aims to analyze phase-amplitude coupling (PAC) patterns during rapid eye movement (REM) sleep in patients with isolated REM sleep behavior disorder (iRBD), compared with demography-matched healthy control (HC) participants.

Methods
At baseline, electroencephalogram data from 13 iRBD patients and 10 HCs during REM sleep were analyzed. During follow-up, four patients (converters) later converted to alpha-synucleinopathies. Phasic and tonic REM states were determined by eye movement in 3 s epochs. PAC was compared between the groups, and correlations with clinical indicators were investigated. Additionally, the contribution of each electrode to PAC components was assessed.

Results
Patients with iRBD exhibited increased delta (1–3 Hz)-gamma (30–50 Hz) PAC only during the phasic REM state, but not during the tonic state, compared to the HCs (p &lt; .05). Elevated PAC in patients negatively correlated with the REM atonia index (p = 0.011) and olfactory function (p = 0.038). Increase PACs were predominent in the fronto-temporo-occipital regions (corrected p &lt; .05). Furthermore, patients showed reduced gamma-amplitude contributions of the parietal region (corrected p &lt; .05). This reduction exhibited a progressively decreasing trend from HC to nonconverters, and further to converters (p for trend = 0.044).

Conclusions
Our findings suggest PAC patterns during REM sleep could provide pathophysiological insights for iRBD. The widespread increase of PAC and reduced gamma-amplitude contribution in the parietal region suggest PAC during phasic REM sleep as potential biomarkers for disease progression in iRBD.
A review of the safety and efficacy of inflatable penile prosthesis ectopic reservoir placement
Authors: Atwater Britney , Krug Aaron , Gross Martin S , Marty-Roix Robyn , Chapin Laura , Morey Allen F 
Published: November 2024

Abstract

Introduction
Patient medical and surgical history factors, such as prior prostatectomy, may lead surgeons to opt for ectopic reservoir placement rather than the standard reservoir location in the retropubic space (RPS) during inflatable penile prosthesis (IPP) placement.

Objective
To examine the safety and effectiveness of ectopic reservoir placement used with three-piece IPPs in relation to reservoir placement in the RPS.

Methods
A systematic review of MEDLINE/Pubmed and Embase databases was performed for literature between 1970 and 2022. Clinical studies and case reports describing three-piece IPP reservoir placement and clinical outcomes on AMS 700, similar products (such as Coloplast Titan), and three-piece IPPs where the manufacturer is not specified were included.

Results
Seventy articles were identified that reported clinical outcomes on three-piece IPP reservoir placement, which included data on 9565 patients. Of these, 67% of the reservoirs (n = 6413) were placed in ectopic locations. These locations were defined as submuscular (n = 5207), retroperitoneal (n = 405), sub-external oblique (n = 50), peritoneal (n = 42), subcutaneous (n = 10), and did not specify the ectopic location (n = 694). A total of 670 patients had ectopic placement of the AMS 700 reservoirs specifically. Overall, there were no elevated rates in safety outcomes between RPS and ectopic placement. Fourteen studies directly compared safety and/or efficacy outcomes between RPS and ectopic placement and did not report any significant differences between patient groups.

Conclusions
Ectopic reservoir placement of three-piece IPPs, including AMS 700, is comparable in terms of safety, efficacy, and patient satisfaction to RPS reservoir placement. Ectopic reservoir placement of the AMS 700 device is also similarly comparable to ectopically placed reservoirs of other IPPs as reported in the literature. Surgeons should consider ectopic implantation for patients at higher risk of complications associated with reservoir placement into the RPS.
A systematic review on the prevalence of a silent female disorder in Arabic-speaking countries and the Middle East: sexual dysfunctions and the role of culture
Authors: Zakhour Stephanie , Gonçalves Walter , Sardinha Aline , Levitan Michelle , Nardi Antonio Egidio 
Published: November 2024

Abstract

Introduction
Female sexual dysfunction (FSD) remains a silent problem, especially in Arabic-speaking countries and the Middle East and most of the data are from Western countries. Sociocultural determinants haven’t been getting the same attention as biological and psychological factors when studying sexual health.

Objectives
To assess the prevalence of FSD in the Arab and Middle Eastern world and understand the sociocultural determinants related.

Methods
On January 13, 2024, we searched electronic databases including Pubmed, Scielo, PsycArticles, Scopus, Cochrane Library and Google Scholar, Middle East Current Psychiatry, and Journal of Middle East Women’s Studies, following PRISMA guidelines. Female participants, aged at least 16 years old from Middle East or Arabic-speaking countries, including North Africa, and cross-sectional studies in Arabic, English, and/or French were included. After careful screening of titles, abstracts, and full texts, ineligible articles were excluded.

Results
A total of 19 papers were included in the present review. The most common FSD in each country were as follows: in Egypt, Turkey, Morocco, and Jordan, desire problems. In Iran, orgasm problems, and pain. In Palestine, pain. In Saudi Arabia, arousal. Sociocultural determinants such as language, poor sexual education, poor sexual self-awareness—and of partners—, lack of training, and help-seeking were related to FSD.

Conclusions
FSD is prevalent in Arabic-speaking countries and the Middle East, yet little to no attention is given to this matter. Causes for FSD are mainly cultural, and Arab and Middle Eastern healthcare professionals lack training. Culturally sensitive, evidence-based studies regarding sexual health need to be conducted.
Administration of sodium hyaluronate to adult horses prior to and immediately after exercise does not alter the range of motion in either the tarsus or metacarpophalangeal joints
Authors: Riley Julia W , Chance Lara M , Barshick Madison R , Johnson Sally E 
Published: November 2024

Abstract
Hyaluronic acid (HA), a glycosaminoglycan found in joint synovial fluid, is administered to horses as an anti-inflammatory with lubrication properties. This experiment examined the effects of HA administered before and shortly after an exercise test on metacarpophalangeal (MCP; fetlock) and tibiotarsal (hock) joint range of motion (ROM). Horses were injected intravenously (IV) with placebo (4 mL, saline) or HA (4 mL, 40 mg) 24 h before performing a standardized exercise test (SET) on a high-speed treadmill and again at 6-h post-SET. Passive fetlock flexion was measured at 4 and 24 h post-SET. Hock flexion and extension were measured at 24 h post-SET by videography and kinematic evaluation at the trot. Parameters of the SET were sufficient to cause peak lactate values of 6.6 ± 0.15 mM and a maximum heart rate of 203.6 ± 4.8 bpm. A minor gain (P = 0.08) in fetlock flexion prior to SET was observed in HA horses that were not retained at either 4 or 24 h post-SET. Hock flexion in both limbs was greater (P &lt; 0.05) at 24 h post-SET, independent of treatment. Horses receiving HA exhibited reduced (P = 0.04) right hock extension. No differences in either right or left hock ROM were observed between control and HA-treated horses. From these results, it is concluded that IV HA injections surrounding an exercise stressor offer no substantive gains in either fetlock or hock ROM.
Unraveling the role of PBK in glioblastoma: from molecular mechanisms to therapeutic targets
Authors: Zhang Yizheng , Luan Mingyuan 
Published: November 2024

Background:
This study investigates the gene expression characteristics of glioma-initiating cells (GIC), an important subgroup of glioblastoma (GBM), after knockdown of PBK (PDZ-binding kinase). Differentially expressed genes (DEGs) between PBK knockdown GIC and control groups were screened through bioinformatics methods. The authors analyzed the mechanisms and roles of these DEGs in GBM tumorigenesis and patient prognosis.

Methods:
Microarray data (GSE53800) were obtained from the Gene Expression Omnibus (GEO) database, selecting 18 GIC cell line samples with or without PBK knockdown. Each control and knockdown group contained three samples. DEGs were screened using R software. GO enrichment analysis, KEGG pathway analysis, PPI network analysis, and hub gene identification were conducted to explore DEG mechanisms. Western blot analysis was also performed to detect EIF4E protein expression, one of the key hub genes, after PBK knockdown in the HS683 glioma cell line.

Results:
A total of 175 upregulated and 145 downregulated genes were identified. GO analysis showed that DEGs were mainly enriched in the positive regulation of cell proliferation, cell adhesion, and angiogenesis. KEGG pathway analysis revealed that DEGs were mainly involved in neuroactive ligand-receptor interactions, calcium signaling, and HIF-1 signaling pathways. Western blot results indicated that EIF4E was downregulated after PBK knockdown.

Conclusion:
A group of genes, such as EIF4E, were closely associated with PBK expression and functions. These findings may provide insight into the molecular mechanism of PBK in GBM.
Cohort profile: The Cancer Risk Associated with the Body Art of Tattooing (CRABAT) study
Authors: Hosseini Bayan , McCarty Rachel , Zins Marie , Goldberg Marcel , Ribet Céline , Schreiver Ines , Ezzedine Khaled , Schüz Joachim , Foerster Milena 
Published: November 2024

AbstractTattooing, involving the injection of pigments into the skin, has become increasingly popular, with up to 40% of individuals under 40 years old tattooed in high-income countries. Despite regulatory measures, tattoo inks may contain hazardous substances such as polycyclic aromatic hydrocarbons, primary aromatic amines, and metallic impurities, many of which are classified as carcinogens. Research on systemic health risks, particularly cancer, associated with intradermal ink exposure remains limited. Complications like contact allergies and inflammatory skin reactions are more frequently reported among tattooed individuals. However, cancer risks from tattooing, especially for internal cancers like lymphoma, are challenging to assess. Existing case-control studies indicate mixed findings regarding hematologic cancers, with one large study reporting a 20% increased lymphoma risk among tattooed individuals in the first two years post-tattooing.Here, we present the Cancer Risk Associated with the Body Art of Tattooing (CRABAT) study, that is, to our knowledge, the first larger study that prospectively investigates tattoo-related cancer risks. CRABAT follows up over 110,000 participants for long-term health effects within the French Constances cohort with objective cancer data linkage. Of ∼13,000 participants that were tattooed in 2020, detailed tattoo exposure assessment via a validated tattoo exposure questionnaire was conducted in 2023 (response rate >60%). With its robust dataset through linkage to the Constances variable pool, CRABAT enables the analysis of exposure-response relationships, and addresses potential confounders such as sociodemographic and lifestyle factors, and alternative pathways such as tattoo-related infections. Body word count: 2498 words
Detection of Epileptic Spasms Using Foundational AI and Smartphone Videos: A Novel Diagnostic Approach for a Rare Neurological Disorder
Authors: Miron Gadi , Halimeh Mustafa , Tietze Simon , Holtkamp Martin , Meisel Christian 
Published: November 2024

AbstractInfantile epileptic spasm syndrome (IESS) is a severe neurological disorder characterized by epileptic spasms (ES). Timely diagnosis and treatment are crucial but often delayed due to symptom misidentification. Smartphone videos can aid in diagnosis, but availability of specialist review is limited. We fine-tuned a foundational video model for ES detection using social media videos, thus addressing this clinical need and the challenge of data scarcity in rare disorders. Our model, trained on 141 children with 991 seizures and 127 children without seizures, achieved high performance (area under the receiver-operating-curve (AUC) 0.96, 83% sensitivity, 95% specificity) including validation on external datasets from smartphone videos (93 children, 70 seizures, AUC 0.98, false alarm rate (FAR) 0.75%) and gold-standard video-EEG (22 children, 45 seizures, AUC 0.98, FAR 3.4%). This study demonstrates the potential of smartphone videos for AI-powered analysis as the basis for accelerated IESS diagnosis and novel strategy for diagnosis of rare disorders.
In vivo genome editing of human hematopoietic stem cells for treatment of blood disorders by mRNA delivery
Authors: Xu Saijuan , Liang Dan , Wang Qiudao , Cheng Yan , Xie Da , Gui Yang , Zhang Haokun , Feng Changrui , Zhao Feiyan , Ren Wendan , Sun Gongrui , Yang Yang , Li Lin , Lai Yongrong , Fu Bin , Lu Yuming , Wang Zi Jun , Wu Yuxuan 
Published: November 2024

SUMMARYEx vivoautologous hematopoietic stem cells (HSCs) gene therapy provides promising new treatments for hematological disorders. However, current methods involve complex processes and chemotherapeutic conditioning, leading to limited accessibility for treatment and significant side effects. Here, we developed an antibody-free targeted lipid nanoparticles (LNPs) for mRNA delivery to HSCsin vivo, enabling efficient base editing of the HBG target in human HSCs to reactivate fetal hemoglobin in derived erythroid cells. Delivery of ABE8e/sgRNA mRNA with optimized structure LNPs achieves efficientin vivobase editing of HBG in transfusion-dependent β-thalassemia (TDT) patients derived HSCs engrafted in immunodeficientNCG-Xmice, showing restored globin chain balance in erythroid cells. Our research indicated that utilizing LNPs for delivery of genome editor achieves efficient editing of endogenous genes of human HSCs. Notably, this non-viral delivery system eliminates the need for harvesting or mobilizing HSCs, providing a potent and one-time treatment potential for blood disorders like sickle cell disease (SCD) and TDT.HighlightsAntibody-free targeted LNPs system achieves efficient mRNA delivery and base editing in human bone marrow cells including HSCs via one-time intravenous injection.Delivery of ABE8e/sgRNA mRNA with optimized LNPs achieves efficientin vivobase editing of HBG in TDT patients derived HSCs engrafted in immunodeficientNCG-Xmice, showing restored globin chain balance in erythroid cells.LNPs efficiently deliver gene editing system to the BM forin vivoediting of human HSCs, providing preclinical evidence for the next generation non-viral gene therapy for blood disorders.
Absorbing molecules make both abdomen and back transparent in live mice
Authors: Tie Xin , Sun Ting , Xiao Guixiu , Zhao Yanjie , Su Jing , Xie Xiaoqi , Yin Wanhong 
Published: November 2024

AbstractThe field of tissue clearing has experienced rapid advancements in the past decades. Tissue clearing techniques primarily rely on physical or chemical approaches to match the refractive indices of cells and their surrounding medium, thereby reducing light scattering and rendering the tissue optically transparent. By doing so, it becomes possible to utilize fluorescence imaging and 3D reconstruction techniques to observe and analyze the contents and structures of tissues at a cellular level. However, effective research on in vivo tissue clearing remains limited due to the involvement of toxic substances and the removal of water or lipids. A recent article inSciencereports a technique that achieves tissue transparency in live animals with absorbing molecules, marking a significant advancement in the field of in vivo tissue clearing. Although this work has great potential for imaging and light-based therapeutics, some labs found it difficult to reproduce the reported results. In this study, we report the successful reproduction of the findings, provide experimental details that we identified as key to reproducibility, present a new transparency effect observed in the back of live mice, which was not shown in the original paper, and offer insights into the future development and applications of in vivo clearing reagents.
TILLCANN: A TILLING platform inCannabis sativafor mutation discovery and crop improvement
Authors: Duarte-Delgado Diana , Alexiou Konstantinos G. , Pujol Marta , Uauy Cristobal , Adamski Nikolai M. , Vidal Victoria , Torres Anthony , Zalewski Christopher , Gaudino Reginald , Monfort Amparo , Argyris Jason 
Published: November 2024

AbstractCultivation ofCannabis sativais increasing because of its therapeutic value and recognition as a multi-purpose and sustainable crop. Targeting Induced Local Lesions in Genomes (TILLING) is a versatile reverse genetics approach that unlocks induced variation through mutagenesis to accelerate the development of new cultivars and contribute to the functional validation of genes. Increasingly efficient next-generation sequencing technologies and genomic resources have combined to make TILLING by sequencing (TbyS) an attractive technique that can be applied in cannabis. Here we describe the development of a mutagenesis protocol forC. sativaand the development of the TILLCANN platform composed of 1,633 M2 families. As a demonstration of the functionality of the platform, we used TbyS to perform a high throughput screening of novel mutations in amplicons for genes associated with important agronomic and biochemical traits in a set of 512 M2 families. We confirmed 14 of the identified mutations and calculated an average mutation frequency range of 1/263 to 1/320 kb. We found that heterozygous mutants in the cannabis homologue of Class IITEOSINTE BRANCHED 1/CYCLOIDEA/PCF(TCP4) are linked to alterations in leaf number and morphology. We expect that the novel genetic variability unlocked in the TILLCANN platform for performing forward or reverse genetic screens can significantly boost breeding programs geared toward both medicinal cannabis and industrial hemp.Gene & accession numbersRaw sequencing data generated in this study was deposited at the European Nucleotide Archive with accession numbers xxx and xxx. Genes and accession numbers discussed within this manuscript correspond to theCannabis sativacs10 genome assembly deposited in National Center for Biotechnology Information with accession number GCA_900626175.2.
A PI3Kδ-Foxo1-FasL signaling amplification loop rewires CD4+T helper cell signaling, differentiation and epigenetic remodeling
Authors: Golec Dominic P , Gazzinelli-Guimaraes Pedro , Chauss Daniel , Nagashima Hiroyuki , Yu Kang , Hill Tom , Nivelo Luis , Cannons Jennifer L , Perry Jillian , Joshi Ilin , Pereira Nicolas , Oliveira Fabrício Marcus Silva , Cruz Anthony C , Druey Kirk M , Lack Justin B , Nutman Thomas B , Villarino Alejandro V , O’Shea John J , Afzali Behdad , Schwartzberg Pamela L 
Published: November 2024

AbstractWhile inputs regulating CD4+T helper cell (Th) differentiation are well-defined, the integration of downstream signaling with transcriptional and epigenetic programs that define Th-lineage identity remain unresolved. PI3K signaling is a critical regulator of T cell function; activating mutations affecting PI3Kδ result in an immunodeficiency with multiple T cell defects. Using mice expressing activated-PI3Kδ, we found aberrant expression of proinflammatory Th1-signature genes under Th2-inducing conditions, bothin vivoandin vitro. This dysregulation was driven by a robust PI3Kδ-IL-2-Foxo1 signaling loop, fueling Foxo1-inactivation, loss of Th2-lineage restriction, altered chromatin accessibility and global impairment of CTCF-DNA interactions. Surprisingly, ablation ofFasl, a Foxo1-repressed gene, restored normal Th2 differentiation, TCR signaling and CTCF expression. BioID revealed Fas interactions with TCR- signaling components, which were supported by Fas-mediated potentiation of TCR signaling. Our results highlight Fas-FasL signaling as a critical intermediate in phenotypes driven by activated-PI3Kδ, thereby linking two key pathways of immune dysregulation.
SenSeqNet: A Deep Learning Framework for Cellular Senescence Detection from Protein Sequences
Authors: Jiang Hanli , Lin Li , Deng Dongliang , Ren Jianyu , Yang Xin , Liu Siyi , Liu Lubin 
Published: November 2024

AbstractCellular senescence, characterized by the irreversible cessation of division in normally proliferating cells due to various stressors, presents a significant challenge in the treatment of age-related diseases. Understanding and accurately detecting cellular senescence is crucial for identifying potential therapeutic targets. However, traditional wet lab assays for detecting cellular senescence are time-consuming and labor-intensive, limiting research and drug development efficiency. There is an urgent need for computational tools allowing swift and accurate detection of cellular senescence from protein sequences. We propose SenSeqNet, a novel deep learning framework for detecting cellular senescence directly from protein sequences. The framework begins with feature extraction using the Evolutionarily Scaled Model (ESM-2), a state-of-the-art protein language model that captures evolutionary information and complex sequence patterns. The extracted embeddings are then passed through a hybrid architecture consisting of long short-term memory (LSTM) networks and convolutional neural networks (CNNs) to further refine and learn from the embedded information. SenSeqNet achieved a final accuracy of 83.55% on independent testing, surpassing various machine learning and deep learning architectures. This performance underscoring the robustness and effectiveness of SenSeqNet for detecting cellular senescence from protein sequences. These results provide a solid foundation for future research on aging and age-related therapeutics.