bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemoresistance of PRAME-expressing melanoma cell can be resolved with help of bortezomib
Authors: Misyurin V. A. , Kalenichenko D. V. , Rudakova A. A. , Finashutina Yu. P. , Lyzhko N. A. , Tikhonova V. V. , Kesaeva L. A. , Solopova O. N. , Misyurina A. Е. , Velikanov A. N. , Baryshnikovа M. А. , Misyurin A. V. 
Published: January 2019

Background.PRAME gene spontaneous expression is frequently observed in a cancer cell. The protein encoded by this gene increases the viability of tumour cell. NF-κB signalling pathway takes part in PRAME upregulation. It proposes, that stress conditions may increase the expression level of PRAME in the tumour cell and increase cell’s viability after it. We hypothesized that this phenomenon determines chemoresistance of PRAME-expressing cell, which can be overcome by NF-κB inhibitors, such as bortezomib.Materials and methods.We incubated A875 melanoma cells with cisplatin, bortezomib and dexamethasone, as well as with a mixture of cisplatin with bortezomib and cisplatin with dexamethasone within 24 hours. To assess the cytotoxicity of these combinations MTT-test was used. For evaluation of PRAME expression level, real-time polymerase chain reaction was used. All data were analyzed with Wilcoxon test for coupled samples.Results.It was found that cisplatin and dexamethasone increased an expression level of PRAME compared to control (p <0.03). The addition of dexamethasone to cisplatin reduced cytotoxic effect of cisplatin. Bortezomib has a cytotoxic effect, but it did not increase the activity of PRAME gene (p = 0.12). PRAME gene activity in cells incubated with a mixture of cisplatin and bortezomib was observed at a lower level in comparison with cells incubated with cisplatin (p = 0.0277).Conclusion.The results of experiments show that an increase of PRAME expression level reduces the sensitivity of melanoma cells to the cytotoxic effect of cisplatin. PRAME activity increases under stress conditions. Using of bortezomib can inhibit the growth of PRAME expression and makes the tumour cell more vulnerable to cytotoxic agents. On the other hand, dexamethasone may increase a resistance of PRAME-expressing cell to cytotoxic effects.
Role of TRIM16 in cancers development
Authors: Spirina L. V. , Kondakova I. V. , Tarasenko N. V. 
Published: January 2019

Тhe protein TRIM16 is involved in key intracellular processes, such as proliferation, cell differentiation and programmed death, including intrinsic and extrinsic apoptosis, autophagy-dependent cell death and immunogenic cell death. The TRIM16 protein acts the proteins TPD43, Gli-1, RARβ, Snail components and MAPK signaling pathway, cadherins, caspases and is also associated with the regulation of the immune system via direct and indirect mechanisms. The influence of TRIM16 protein on the pathogenesis of hormone-dependent tumors is well-known. Further study of the TRIM16 role in the development and progression of malignant neoplasms will form the basis for the development of new methods for predicting the course of the malignant process.
Noticias relativas a la música y los músicos, procedentes de las Actas Capitulares de la iglesia de Santa María del Pilar de Zaragoza, 1600-1656
Authors: Ezquerro Esteban Antonio 
Published: January 2019

Las noticias musicales de la primera mitad del siglo XVII procedentes de las actas capitulares de la hoy catedral basílica de Nuestra Señora del Pilar de Zaragoza, eran inéditas hasta ahora. La aparición, el 20.05.2004 del volumen de actas correspondiente a los años 1614-1656, no disponible para la investigación hasta dicha fecha, ha motivado el interés musicológico por recuperar noticia de las actividades musicales y de los músicos que protagonizaron aquel período del Siglo de Oro de las artes y las letras hispano, en el que la corte habsbúrgica visitara la capital aragonesa en diversas ocasiones, contribuyendo, de ese modo, a un incremento popular de la devoción hacia dicho templo y su significación. Un ascenso de la devoción popular que, a partir de la tradición legendaria de la aparición de María al apóstol Santiago (en el año 40 de nuestra Era, encaramada a un pilar a orillas del río Ebro), había convertido al templo mariano zaragozano en un notable centro de peregrinación, a lo que se uniría el denominado “milagro de Calanda”, la declaración de Santa María del Pilar como patrona primero de la ciudad de Zaragoza y enseguida del Reino de Aragón, y que iba a desembocar, ya el 11.02.1676 en la bula del papa Clemente X <em>In apostolicae dignitatis.</em> En virtud de esta última, los cabildos zaragozanos de la iglesia metropolitana del Salvador (vulgo “La Seo”) y el de Santa María de El Pilar, se unían definitivamente bajo la autoridad de un único arzobispo de Zaragoza, alcanzando, de ese modo, el templo de Santa María el rango de catedral. En consecuencia, en esta documentación aparecen mencionados nombres de maestros de capilla, organistas y otros músicos, a la que se añaden ahora también los correspondientes a los años 1600-1613, estableciendo así, documentalmente, la nómina de protagonistas músicos de la iglesia y su fijación cronológica.
Morphofunctional Characteristics of the Stomach Exposed to Sodium Tetraborate
Authors: Berdalinova A. K. , Umbetov T. Zh. , Shevlyuk N. N. , Zhurabekova G. A. 
Published: January 2019

The aim is to study the morphological and functional characteristics of the gastric mucosa of rats exposed to sodium tetraborate.Material and methods. The object of the study were the stomachs of 50 white male mongrel rats. The animals were divided into 2 groups. Animals of the 1st group once intragastrically through the probe entered the borax in the dose of LD50– 5000 mg/kg. Animals of the second group intragastrically through a tube daily introduced sodium tetraborate at a dose of 1/10 LD50– 500 mg/kg. the Animals of the 1stgroup were withdrawn from the experiment in a day after single administration of sodium tetraborate, animals of the 2ndgroup – 7, 14, 21 and 30 days after the start of the experiment. The obtained material was treated using histological, histochemical and immunocytochemical methods.Results. With a single and long-term exposure to sodium tetraborate in the gastric mucosa against the background of edema, stagnation of blood elements in the blood vessels of the microcirculatory bed, moderate leukocyte infiltration, there was focal desquamation of the epithelium, numerous destructive changes in the cover and glandular cells of the gastric mucosa, a significant death of epithelial cells, accompanied by a decrease in the secretory function of mucous cells. Destructive changes are more pronounced at a higher dose of sodium tetraborate. Damage to epithelial cells lead to an increase in the zone of proliferative activity of the epithelium of the mucous membrane (mitoses are noted not only in the neck of the gland, but also in the body of the gland and in the epithelium of gastric pits). In the own plate of the mucous membrane there is an increase in the activity of fibroblasts.Conclusion. The data obtained indicate a negative impact of the used doses of sodium tetraborate. The expansion of the zone of proliferative activity of the epithelium of the mucous membrane of the stomach, aimed at the restoration of structural homeostasis, is not fully offset with a significant destruction of epithelial and glandular cells. The increase in the activity of fibroblastic differon cells indicates their greater resistance to the negative effects of sodium tetraborate in comparison with epithelial cells.
Structure Features and Sensitivity of Hippocampal Cells to the Endothelial Vascular Growth Factor Against the Background of Intranasal Administration of Titanium Dioxide Nanoparticles
Authors: Sharafutdinova L. A. , Valiullin V. V. 
Published: January 2019

The aim:to study the structural characteristics and sensitivity of rat hippocampal cells to the endothelial growth factor of vessels against intranasal administration of nanoparticles (NP) TiO2(rutile form, 40–60 nm, daily, 10 mg/kg animal body weight, 30 days).Material and methods.When studying the effect of the studied NP on the morphological characteristics of the hippocampus, animals of the experimental group were intranasally injected daily with a suspension of NP TiO2(10 mg/kg body weight, 30 days), and the rats of the control group received distilled water in the same volume. Animals were removed from the experiment after 30 days of overdose of diethyl ether and the brain was removed for histological and electron microscopic examination. We used classical morphological approaches, electron microscopy and immunohistochemical staining with antibodies to the cell sensitivity marker, the receptor for endothelial vascular growth factor VEGFR-1 / Flt-1.The classical morphological approaches, the method of electron microscopy and immunohistochemical staining with antibodies to the marker of sensitivity of the receptor cells to the endothelial vascular growth factor VEGFR-1/Flt-1 were used.Results. It has been established that against the background of intranasal administration of NP TiO2in the pyramidal layer of CA1and CA3hippocampal fields, the density of neuronal packing decreases, the area of the nucleus and the pericarion of the cells decrease in comparison with the control. Ultrastructural changes were detected, indicating a damage to the structures of the energy and protein synthesis apparatus of neurons. Immunohistochemical study revealed an increase in the sensitivity of vascular endothelial cells and the hippocampal macroglia of the experimental group of animals to the endothelial factor of vascular growth.Conclusions. Intranasal management of NP TiO2leads to negative changes in the structural characteristics of the CA1, CA3hippocampal zones, ultramicroscopic features, and also to an increase in the sensitivity of a number of cells to VEGF, which indicates their possible neurotoxic effect.
MENYOAL PROFESIONALISME GURU PROFESIONAL: SEBUAH TELAAH KRITIS
Authors: Almu'tasim Amru 
Published: January 2019

<p><em>Teacher is a designation for the position, position and profession for someone who devoted himself in the field of education through educational interaction patterned, formal, and systematic (Surya, 1998). Historically professorship connote uplifted services (noblest vocation). They named the child paedagogos or waiters, waitresses humanizing honorable man, or man of human beings (gogos humanities). Guru is one of the human component in the teaching-learning process, which had a role in the formation of human resources business potential in development. Therefore, teachers must be someone who can 'digugu' and 'imitated'. Teachers must participate actively and putting his position as professionals, in accordance with the demands of the growing community.</em></p><p><strong><em> </em></strong></p><p><strong><em>Keywords: </em></strong><em>professionalism, professional teacher</em><strong><em></em></strong></p>
KAJIAN TEMATIS Al-QUR’AN DAN HADITS TENTANG KEPEMIMPINAN
Authors: Pramitha Devi 
Published: January 2019

<p><em>Leaders and leadership are two interrelated problems of daily life in society, organization, nation and state. The development level of society, organizations and nations and countries influenced by the leaders and leadership. Islam itself as a religion rahmatan lil 'Alamin also put the issue of leaders and leadership as one of the major issues in his teaching. In the Qur'an and Hadith leadership gets a share of the discussion is not small, many verses of the Koran and the hadiths of the Prophet who Membincang about leadership. In the Islamic concept itself, leadership can be defined as a concept of interaction, relationships, processes of authority, influence activities, directs and coordinates both horizontally and vertically.</em></p><p><strong><em> </em></strong></p><p><strong><em>Keywords: </em></strong><em>leadership, al-Qur’an, hadith<strong></strong></em></p>
PENINGKATAN MUTU SKL SISWA SMK MELALUI MODEL YANG BERORIENTASI PADA KEMAMPUAN PERANCANGAN DAN MELAKUKAN EKSPERIMEN UNTUK MELATIH KETRAMPILAN BERPIKIR KRITIS
Authors: Maimunah Siti Annijat 
Published: January 2019

<p><em>The legislation System of national education No. 20 Year 2003 about education standards consist of standard content, processes, the competence of graduates, educational personnel, infrastructure, management, financing and assessment of education.</em></p><p><em>Standards of competence and standard of graduates, Graduates in Vocational secondary schools are expected to have the ability to build Standard and competence of graduates of the creative, and innovatively in decision making. The reality of the situation of vocational high school graduates do not meet the standard of competence of Graduates, because the existence of limited practical tools so that the experiment also rarely do. </em></p><p><em>The purpose of the study is to describe the practicality, and the effectiveness of the implementation of the model of learning ability-oriented design and conduct experiments to practice critical thinking skills of students of SMK.</em></p><p><em>As for the approach of this study is a descriptive qualitative. Based on the results of the implementation of the developed learning the real device, it can be concluded that the ability of the design-oriented learning and experimentation to train creative and critical thinking skills a valid, practical, effective, and can enhance critical thinking skills and creative as well as achieving perfection results of student learning. Positive student response after follow learning by applying the learning ability-oriented device design and perform experiments to practice critical thinking skills and creative students.</em></p><p><em> </em></p><p><strong><em>Key words:</em></strong><em> quality improvement of the standard of competence of graduates of vocational high school Students, the ability of Designing and performing experiments, Thinking Skills</em></p>
Effects of liquid ionic silver concentration on caspase-3 and p53mt expressions in the oral mucosal epithelium of Wistar rats
Authors: Muharram R. Aries , Istiati I. , Soesilawati Pratiwi 
Published: January 2019

Background: Silver, especially oxidized silver, has been used as a medicine considered to have bactericidal properties. In the present day, ionic silver (Ag+) is also used in the manufacture of cosmetics, socks, food containers, detergents, sprays and other products to prevent the spread of germs. Unfortunately, ionic silver is assumed to be toxic not only to bacteria, but also to humans and the environment. Therefore, it is essential to identify the optimum dosage of ionic silver considered safe by investigating the effects of ionic silver concentration on cell death through activation of mutant p-53 expression by caspase 3 in the oral epithelium. Purpose: This research aimed to analyze the effects of concentrated liquid ionic silver (Ag+) on caspase-3 and mutant p53 expression in the oral mucosal epithelium. Methods: This research constituted a laboratory-based experimental study with posttest-only design. The research subjects consisted of 28 Wistar rats, divided into four treatment groups, namely; KK (with Aquadest), KP 1 (with 5% liquid ionic silver), KP 2 (with 10% liquid ionic silver) and KP 3 (with 15% liquid ionic silver). Each rat was then treated orally with 0.5ml of liquid ionic silver at fixed concentrations twice a day for seven days. The Wistar rats were then terminated and their tissue samples processed by means of histopathological and immunohistochemical staining examination. The monoclonal Caspase-3 and mutant p53 expressions in each group were evaluated with the data being tabulated and analyzed statistically. Results: Mutant p53 expression was also found in the control group. Moreover, the higher the concentration of liquid ionic silver, the greater the elevated Caspase-3 and mutant p53 expressions. Conclusion: The concentration of liquid ionic silver plays an important role in elevating Caspase-3 and mutant p53 expressions.
Herbal-induced Stevens-Johnson syndrome with oral involvement and management in an HIV patient
Authors: Suniti S. , Sufiawati Irna 
Published: January 2019

Background: Stevens-Johnson syndrome (SJS) is an immune complex-mediated hypersensitivity reaction affecting the skin and mucous membranes. Patients infected with human immunodeficiency virus (HIV) are at increased risk of developing SJS which is predominantly caused by an adverse reaction to medications, including herbal varieties. In recent years, the consumption of herbal medicines has increased, while their safety remains a matter for investigation. Purpose: The purpose of this case report is to explain the occurrence of SJS caused by herbal medicine. Case: A 43-year-old male patient with body-wide skin erosion was referred to the Department of Oral Medicine and subsequently diagnosed with Stevens-Johnson syndrome due to his consumption of a herbal medicine containing zingiber rhizoma, coboti rhizoma, asari herbal and epimedi. The patient’s chief complaints included difficulty when opening the mouth, dysphagia and excessive production of saliva continuously contaminated with blood and sputum. Extraoral examination showed a sanguinolenta crust on the lips. Intra oral examination of oral mucous showed erosive lesions with bleeding and pain. A HIV test performed at a Clinical Pathology Laboratory was positive for antibodies against HIV with a CD4 cell count of 11 cells/ml. Case management: Treatment consisted of the administering of NaCl 0.9 %, hydrocortisone 0.1% and Chlorhexidine digluconate 0.12% for 12 days. Conclusion: SJS can be caused by herbal medicine and it is essential to be aware of the latter’s potential adverse effects, especially in immunocompromised patients. Symptomatic management of oral lesions should be planned as an early intervention in order to decrease morbidity and mortality in SJS patients.
Description of Longidorus azarbaijanensis n. sp. (Dorylaimida: Longidoridae) from Iran
Authors: Gharibzadeh Farshad , Pourjam Ebrahim , Pedram Majid 
Published: January 2019

Abstract

Longidorus azarbaijanensis n. sp. is described and illustrated using morphological and molecular data. It was recovered in West Azarbaijan province, northwestern Iran, from the rhizospheric soil of foxtail weed. The new species is characterized by having 5.4 to 6.8 mm long females, offset, anteriorly flat lip region and separated from the rest of the body by a shallow constriction, funnel-shaped amphidial pouches, the guiding ring at 23 to 27 µm from the anterior end, 73 to 81 and 44 to 50 µm long odontostyle and odontophore, respectively, 95.0 to 113.5 µm long pharyngeal bulb, didelphic-amphidelphic reproductive system with long tubular uteri lacking sperm cells, vulva located at 52.0% to 56.4%, conical tail dorsally convex, ventrally flat, with bluntly rounded wide tip, for juvenile developmental stages and absence of males. The general body shape of the new species is similar to that of five known species of the genus namely L. euonymus, L. perangustus, L. persicus, L. protae and L. sturhani. The morphological differences of the new species with the aforementioned species are discussed. For all the aforementioned species (except L. protae, currently lacking molecular data) the differences of the new species was also confirmed with differences in molecular sequences of D2-D3 expansion domains of 28S rDNA and the corresponding phylogenetic analyses. The partial sequence of the internal transcribed spacer 1 (ITS1) of the new species was also used in phylogenetic analyses. In partial 28S tree, the clade including the new species and six other species (L. attenuatus, Longidorus sp. and four above-mentioned species having molecular data for this fragment) was well supported in Bayesian inference. In the ITS1 tree, the new species formed a clade with L. euonymus, L. perangustus and L. persicus, as in 28S tree. This is one of the cases from which the morphologically similar species are separated using molecular sequences.
Morphological and Molecular Characterization of Paralongidorus sali Siddiqi, Hooper, and Khan, 1963 with a Description of the First-Stage Juvenile and Male of Longidorus jonesi Siddiqi, 1962 from China
Authors: Cai Ruihang , Maria Munawar , Qu Nan , Castillo Pablo , Zheng Jingwu 
Published: January 2019

Abstract
Longidorids are economically important plant-parasitic nematodes because several species are virus vectors. Populations of Paralongidorus sali and Longidorus jonesi, isolated from woody perennials of Hangzhou, Zhejiang, China, were characterized molecularly and morphologically. The morphometric data of the Chinese populations of both species were compared with other populations. The present study provided a first record of the occurrence of Paralongidorus in China coupled with description of the first-stage Juvenile and male of L. jonesi. Phylogenetic analysis based on 18S and D2–D3 expansion segments of 28S gene indicated that L. jonesi clustered with L. jonesi reported from Japan and P. sali grouped with P. bikanerensis from Iran. Considering the pathological and economic importance of this group of nematodes, the study emphasized the need of updated descriptions from accurately identified specimens, isolation of sufficient material for examination, and molecular and phylogenetic analysis for a better understanding and diagnostics of Longidorid nematodes.
Nematicidal Weeds, Solanum nigrum and Datura stramonium
Authors: Oplos Chrisostomos , Eloh Kodjo , Spiroudi Urania-Menkissoglu , Pierluigi Caboni , Ntalli Nikoletta 
Published: January 2019

Abstract
We investigated Solanum nigrum (seeds) and Datura stramonium (shoots) against root-knot nematodes in terms of J2 paralysis and egg hatch inhibition (methanol extract), as well as inhibition of nematode development in host roots (soil amending with either S. nigrum seeds’ or D. stramonium shoots’ meal). Datura stramonium was found equally effective at inhibiting motility of Meloidogyne incognita and Meloidogyne javanica (both EC50 = 427 μg mL−1 at 3 day), but inhibition occurred more quickly for M. incognita (1 day). Solanum nigrum was faster and more effective at inhibiting motility of M. incognita than M. javanica (EC50 = 481 and 954 μg mL−1 at 3 day, respectively). Datura stramonium was slower, but eventually more potent in decreasing egg hatch and cell division in M. incognita eggs, than S. nigrum. Specifically, D. stramonium significantly inhibited cell division in eggs immersed in at least 100 and 1 μg mL−1 at Day 6 and 10, respectively. Solanum nigrum impeded cell division in un-differentiated eggs immersed in not less than 10 and 100 μg mL−1 after days 2 and 6, respectively. Both extracts were similar in suppressing J2 exclosure but D. stramonium was effective in smaller test concentrations. Specifically, D. stramonium suppressed J2 emerging from eggs immersed in 10 μg mL−1 at day2, and in at least 1 μg mL−1 at day 6. Solanum nigrum significantly reduced J2 hatch from eggs immersed in a minimum of 100 μg mL−1 at day 2 and not less than 1,000 μg mL−1 at day 6. In pots, powdered S. nigrum seeds meal was more active than D. stramonium and the respective EC50 females/g values for M. incognita were 1.13 and 11.4 mg g−1 of soil, respectively. The chemical composition of active extracts was determined after derivatization by GC–MS. Chemical analysis of active extracts showed the presence of fatty acids with known nematicidal activity.