bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visible‐Light‐Driven Hydrogen Production and Polymerization using Triarylboron‐Functionalized Iridium(III) Complexes
Authors: Yang Ling‐Xia , Yang Wan‐Fa , Yuan Yong‐Jun , Su Yi‐Bing , Zhou Miao‐Miao , Liu Xiao‐Le , Chen Guang‐Hui , Chen Xin , Yu Zhen‐Tao , Zou Zhi‐Gang 
Published: May 2018

AbstractThe development of novel iridium(III) complexes has continued as an important area of research owing to their highly tunable photophysical properties and versatile applications. In this report, three heteroleptic dimesitylboron‐containing iridium(III) complexes, [Ir(p‐B‐ppy)2(N^N)]+ {p‐B‐ppy=2‐(4‐dimesitylborylphenyl)pyridine; N^N=dipyrido[3,2‐a:2′,3′‐c]phenazine (dppz) (1), dipyrido[3,2‐d:2′,3′‐f]quinoxaline (dpq) (2), and 1,10‐phenanthroline (phen) (3)}, were prepared and fully characterized electrochemically, photophysically, and computationally. Altering the conjugated length of the N^N ligands allowed us to tailor the photophysical properties of these complexes, especially their luminescence wavelength, which could be adjusted from λ=583 to 631 nm in CH2Cl2. All three complexes were evaluated as visible‐light‐absorbing sensitizers for the photogeneration of hydrogen from water and as photocatalysts for the photopolymerization of methyl methacrylate. The results showed that all of them were active in both photochemical reactions. High activity for the photosensitizer (over 1158 turnover numbers with 1) was observed, and the system generated hydrogen even after 20 h. Additionally, poly(methyl methacrylate) with a relatively narrow molecular‐weight distribution was obtained if an initiator (i.e., ethyl α‐bromophenylacetate) was used. The living character of the photoinduced polymerization was confirmed on the basis of successful chain‐extension experiments.
Palladium‐Catalyzed ortho C−H Arylation of Benzaldehydes Using ortho‐Sulfinyl Aniline as Transient Auxiliary
Authors: Mu Delong , He Gang , Chen Gong 
Published: May 2018

AbstractA PdII‐catalyzed ortho‐(Csp2)–H arylation reaction of benzaldehydes using a catalytic amount of 2‐(methylsulfinyl)aniline as transient auxiliary was developed. This reaction is compatible with a broad range of benzaldehyde and aryl iodide substrates. Compared with other related reaction systems, an excellent regioselectivity for ortho‐C(sp2)−H bonds over benzylic C(sp3)−H bonds was obtained for ortho‐alkyl‐benzaldehyde substrates.
A reference genome of the Chinese hamster based on a hybrid assembly strategy
Authors: Rupp Oliver , MacDonald Madolyn L. , Li Shangzhong , Dhiman Heena , Polson Shawn , Griep Sven , Heffner Kelley , Hernandez Inmaculada , Brinkrolf Karina , Jadhav Vaibhav , Samoudi Mojtaba , Hao Haiping , Kingham Brewster , Goesmann Alexander , Betenbaugh Michael J. , Lewis Nathan E. , Borth Nicole , Lee Kelvin H. 
Published: April 2018

AbstractAccurate and complete genome sequences are essential in biotechnology to facilitate genome‐based cell engineering efforts. The current genome assemblies for Cricetulus griseus, the Chinese hamster, are fragmented and replete with gap sequences and misassemblies, consistent with most short‐read‐based assemblies. Here, we completely resequenced C. griseus using single molecule real time sequencing and merged this with Illumina‐based assemblies. This generated a more contiguous and complete genome assembly than either technology alone, reducing the number of scaffolds by >28‐fold, with 90% of the sequence in the 122 longest scaffolds. Most genes are now found in single scaffolds, including up‐ and downstream regulatory elements, enabling improved study of noncoding regions. With >95% of the gap sequence filled, important Chinese hamster ovary cell mutations have been detected in draft assembly gaps. This new assembly will be an invaluable resource for continued basic and pharmaceutical research.
Non‐Newtonian rheology in suspension cell cultures significantly impacts bioreactor shear stress quantification
Authors: Wyma Alex , Martin‐Alarcon Leonardo , Walsh Tylor , Schmidt Tannin A. , Gates Ian D. , Kallos Michael S. 
Published: April 2018

AbstractThe fields of regenerative medicine and tissue engineering require large‐scale manufacturing of stem cells for both therapy and recombinant protein production, which is often achieved by culturing cells in stirred suspension bioreactors. The rheology of cell suspensions cultured in stirred suspension bioreactors is critical to cell growth and protein production, as elevated exposure to shear stress has been linked to changes in growth kinetics and genetic expression for many common cell types. Currently, little is understood on the rheology of cell suspensions cultured in stirred suspension bioreactors. In this study, we present the impact of three common cell culture parameters, serum content, cell presence, and culture age, on the rheology of a model cell line cultured in stirred suspension bioreactors. The results reveal that cultures containing cells, serum, or combinations thereof are highly shear thinning, whereas conditioned and unconditioned culture medium without serum are both Newtonian. Non‐Newtonian viscosity was modeled using a Sisko model, which provided insight on structural mechanisms driving the rheological behavior of these cell suspensions. A comparison of shear stress estimated by using Newtonian and Sisko relationships demonstrated that assuming Newtonian viscosity underpredicts both mean and maximum shear stress in stirred suspension bioreactors. Non‐Newtonian viscosity models reported maximum shear stresses exceeding those required to induce changes in genetic expression in common cell types, whereas Newtonian models did not. These findings indicate that traditional shear stress quantification of cell or serum suspensions is inadequate and that shear stress quantification methods based on non‐Newtonian viscosity must be developed to accurately quantify shear stress.
A novel plasma circular RNA circFARSA is a potential biomarker for non‐small cell lung cancer
Authors: Hang Dong , Zhou Jing , Qin Na , Zhou Wen , Ma Hongxia , Jin Guangfu , Hu Zhibin , Dai Juncheng , Shen Hongbing 
Published: May 2018

AbstractEmerging evidence indicates that circular RNAs (circRNAs) are implicated in cancer development. This study aimed to evaluate whether circulating circRNAs may serve as novel biomarkers for non‐small cell lung cancer (NSCLC). We used RNA sequencing (RNA‐seq) and quantitative real‐time PCR to explore cancer‐related circRNAs. Bioinformatics and functional analyses were performed to reveal biological effects of circRNAs on lung cancer cells. A total of 5471 distinct circRNAs were identified by total RNA‐seq, in which 185 were differentially expressed between cancerous and adjacent normal tissues. A circRNA derived from exon 5–7 of the FARSA gene, termed circFARSA, was observed to increase in cancerous tissues (P = 0.016), and was more abundant in patients’ plasma than controls (P < 0.001). Overexpression of circFARSA in A549 cell line significantly promoted cell migration and invasion. In silico analysis suggested that circFARSA might sponge miR‐330‐5p and miR‐326, thereby relieving their inhibitory effects on oncogene fatty acid synthase. Summarily, this study revealed circRNA profile of NSCLC for the first time and provided the evidence of plasma circFARSA as a potential noninvasive biomarker for this malignancy.
The Alkylquinolone Repertoire of Pseudomonas aeruginosa is Linked to Structural Flexibility of the FabH‐like 2‐Heptyl‐3‐hydroxy‐4(1H)‐quinolone (PQS) Biosynthesis Enzyme PqsBC
Authors: Witzgall Florian , Depke Tobias , Hoffmann Michael , Empting Martin , Brönstrup Mark , Müller Rolf , Blankenfeldt Wulf 
Published: May 2018

AbstractPseudomonas aeruginosa is a bacterial pathogen that causes life‐threatening infections in immunocompromised patients. It produces a large armory of saturated and mono‐unsaturated 2‐alkyl‐4(1H)‐quinolones (AQs) and AQ N‐oxides (AQNOs) that serve as signaling molecules to control the production of virulence factors and that are involved in membrane vesicle formation and iron chelation; furthermore, they also have, for example, antibiotic properties. It has been shown that the β‐ketoacyl‐acyl‐carrier protein synthase III (FabH)‐like heterodimeric enzyme PqsBC catalyzes the last step in the biosynthesis of the most abundant AQ congener, 2‐heptyl‐4(1H)‐quinolone (HHQ), by condensing octanoyl‐coenzyme A (CoA) with 2‐aminobenzoylacetate (2‐ABA), but the basis for the large number of other AQs/AQNOs produced by P. aeruginosa is not known. Here, we demonstrate that PqsBC uses different medium‐chain acyl‐CoAs to produce various saturated AQs/AQNOs and that it also biosynthesizes mono‐unsaturated congeners. Further, we determined the structures of PqsBC in four different crystal forms at 1.5 to 2.7 Å resolution. Together with a previous report, the data reveal that PqsBC adopts open, intermediate, and closed conformations that alter the shape of the acyl‐binding cavity and explain the promiscuity of PqsBC. The different conformations also allow us to propose a model for structural transitions that accompany the catalytic cycle of PqsBC that might have broader implications for other FabH‐enzymes, for which such structural transitions have been postulated but have never been observed.
Aptamer‐Functionalized Magnetic Conjugated Organic Framework for Selective Extraction of Traces of Hydroxylated Polychlorinated Biphenyls in Human Serum
Authors: Jiang Dandan , Hu Tingting , Zheng Haijiao , Xu Guoxing , Jia Qiong 
Published: May 2018

AbstractA solid‐phase extraction adsorbent based on an aptamer‐functionalized magnetic conjugated organic framework (COF) was developed for selective extraction of traces of hydroxylated polychlorinated biphenyls. This material has advantages such as superparamagnetism of the magnetic core, high surface area and porous structure of the COF, and high specific affinity of the aptamer. In combination with HPLC‐MS, the aptamer‐functionalized magnetic COF was used for the capture of hydroxy‐2′,3′,4′,5,5′‐pentachlorobiphenyl in human serum. The method provided a linear range of 0.01–40 ng mL−1 with a good correlation coefficient (R2=0.9973). The limit of detection was as low as 2.1 pg mL−1. Furthermore, the material showed good reusability and could be applied in at least ten extraction cycles with recoveries greater than 90 %.
Selective On/Off‐Nitroxides as Radical Probes to Investigate Non‐radical Enzymatic Activity by Electron Paramagnetic Resonance
Authors: Duttagupta Indranil , Jugniot Natacha , Audran Gérard , Franconi Jean‐Michel , Marque Sylvain R. A. , Massot Philippe , Mellet Philippe , Parzy Elodie , Thiaudière Eric , Vanthuyne Nicolas 
Published: May 2018

AbstractA nitroxide carrying a peptide specific to the binding pocket of the serine proteases chymotrypsin and cathepsin G is prepared. This peptide is attached as an enol ester to the nitroxide. Upon enzymatic hydrolysis of the peptide, the enol ester moiety is transformed into a ketone moiety. This transformation affords a difference of 5 G in phosphorus hyperfine coupling constant between the electronic paramagnetic resonance (EPR) signals of each nitroxide. This property is used to monitor the enzymatic activity of chymotrypsin and cathepsin G by EPR. Michaelis constants were determined and match those reported for conventional optical probes.
Functionalization of Bambusurils by a Thiol–Ene Click Reaction and a Facile Method for the Preparation of Anion‐Free Bambus[6]urils
Authors: Azazna Djamille , Lafosse Marine , Rivollier Julie , Wang Jialan , Cheikh Imen Ben , Meyer Michel , Thuéry Pierre , Dognon Jean‐Pierre , Huber Gaspard , Heck Marie‐Pierre 
Published: May 2018

AbstractSulfide‐functionalized bambus[4]urils ((RS)8BU[4]) and bambus[6]urils ((RS)12BU[6]) were synthesized through thiol–ene click coupling reactions (TEC) of allylbambus[n]urils. Thiosugars were grafted to BU[4] and BU[6]. Synthesis of BU[6] derivatives always requires the use of a template anion (iodide, chloride, or bromide), which is enclosed in the cavity of BU[6]. We show that this anion influences the reactivity of bambus[6]urils. An encapsulated iodide makes allyl functions of allyl12BU[6] less reactive towards TEC and hydrogenation reactions in comparison to the corresponding chloride or bromide inclusion complexes. This is critical for the chemical reactivity of BU[6] and even more to determine their anion‐binding properties. We report a new, facile and fast method using AgSbF6 to prepare anion‐free BU[6]. NMR spectroscopic methods were used to estimate association constants of these new empty BU[6] with different anions. Quantum chemical calculations were employed to rationalize the observed results. These new functionalized bambusuril scaffolds in alternate conformations could find applications as multivalent binders.
Risk factors for locoregional disease recurrence after breast‐conserving therapy in patients with breast cancer treated with neoadjuvant chemotherapy: An international collaboration and individual patient meta‐analysis
Authors: Valachis Antonios , Mamounas Eleftherios P. , Mittendorf Elizabeth A. , Hayashi Naoki , Ishitobi Makoto , Natoli Clara , Fitzal Florian , Rubio Isabel T. , Tiezzi Daniel G. , Shin Hee‐Chul , Anderson Stewart J. , Hunt Kelly K. , Matsuda Naoko , Ohsumi Shozo , Totomi Athina , Nilsson Cecilia 
Published: May 2018

BACKGROUNDSeveral studies have reported a high risk of local disease recurrence (LR) and locoregional disease recurrence (LRR) in patients with breast cancer after neoadjuvant chemotherapy (NCT) and breast‐conserving therapy (BCT). The objective of the current study was to identify potential risk factors for LR and LRR after NCT and BCT.METHODSIndividual patient data sets from 9 studies were pooled. The outcomes of interest were the occurrence of LR and/or LRR. A 1‐stage meta‐analytic approach was used. Cox proportional hazards regression models were applied to identify factors that were predictive of LR and LRR, respectively.RESULTSA total of 9 studies (4125 patients) provided their data sets. The 10‐year LR rate was 6.5%, whereas the 10‐year LRR rate was 10.3%. Four factors were found to be associated with a higher risk of LR: 1) estrogen receptor‐negative disease; 2) cN + disease; 3) a lack of pathologic complete response in axilla (pN0); and 4) pN2 to pN3 disease. The predictive score for LR determined 3 risk groups: a low‐risk, intermediate‐risk, and high‐risk group with 10‐year LR rates of 4.0%, 7.9%, and 20.4%, respectively. Two additional factors were found to be associated with an increased risk of LRR: cT3 to cT4 disease and a lack of pathologic complete response in the breast. The predictive score for LRR determined 3 risk groups; a low‐risk, intermediate‐risk, and high‐risk group with 10‐year LRR rates of 3.2%, 10.1%, and 24.1%, respectively.CONCLUSIONSBCT after NCT appears to be an oncologically safe procedure for a large percentage of patients with breast cancer. Two easy‐to‐use clinical scores were developed that can help clinicians to identify patients at higher risk of LR and LRR after NCT and BCT and individualize the postoperative treatment plan and follow‐up. Cancer 2018;124:2923‐30. © 2018 American Cancer Society.
Transformation of Indium and Gallium Metal into Mixed Group 11/13 Ternary Sulfide Nanoparticles by Using a Dithioic Acid
Authors: Senevirathna Dimuthu C. , Spiccia Leone , Werrett Melissa V. , Andrews Philip C. 
Published: April 2018

AbstractHeterobimetallic Group 11/13 sulfide nanoparticles (AgInS2, CuInS2, Ag9GaS6, and CuGaS2) are formed by treatment of [M(S2CAr)3] (M=Ga or In) with either AgNO3 or CuCl under mild conditions. The intermediary gallium or indium tris(aryldithioate) complexes are easily prepared by stirring the appropriate metal and aryldithioc acid at room temperature. Overall, this two‐step process is a simple solution‐based method for transforming Ga and In metal into valuable ternary metallosulfide nanoparticles at relatively low temperatures.
A therapist version of the Alliance Negotiation Scale
Authors: Doran Jennifer M. , Gómez‐Penedo Juan Martín , Safran Jeremy D. , Roussos Andrés 
Published: May 2018

AbstractThe aim of the current study was to design and evaluate a therapist version of the Alliance Negotiation Scale (ANS). The ANS was created in order to operationalize the construct of dyadic negotiation in psychotherapy and to augment existing conceptualizations of the working alliance. The ANS has existed only as a client self‐report form since its inception and has demonstrated promise as a psychotherapy process measure. This research intended to develop a complementary therapist self‐report version of the measure. The scale creation process is discussed in detail, and the results of a preliminary psychometric investigation are reported. The ANS‐Therapist version (ANS‐T) was developed using a sample of therapists (n = 114) through a principal components analysis procedure. The ANS‐T contains 9 unidimensional items and was moderately correlated with therapist‐reported working alliance (r = .468). The results of the study support the composition of the ANS‐T and provide initial support for the reliability and validity of the measure.
Mode selection schemes for unicasting device‐to‐device communications supported by network coding
Authors: Fodor Gabor 
Published: May 2018

SummaryDevice‐to‐device (D2D) communication in a cellular spectrum increases the spectral and energy efficiency of local communication sessions, while also taking advantage of accessing licensed spectrum and higher transmit power levels than when using unlicensed bands. To realize the potential benefits of D2D communications, appropriate mode selection algorithms that select between the cellular and D2D communication modes must be designed. On the other hand, physical‐layer network coding (NWC) at a cellular base station—which can be used without D2D capability—can also improve the spectral efficiency of a cellular network that carries local traffic. In this paper, we ask whether cellular networks should support D2D communications, physical‐layer NWC, or both. To this end, we study the performance of mode selection algorithms that can be used in cellular networks that use physical‐layer NWC and support D2D communications. We find that the joint application of D2D communication and NWC scheme yields additional gains compared with a network that implements only one of these schemes, provided that the network implements proper mode selection and resource allocation algorithms. We propose 2 mode selection schemes that aim to achieve high signal‐to‐interference‐plus‐noise ratio and spectral efficiency, respectively, and take into account the NWC and D2D capabilities of the network.
Lack of transformation zone in cervical Pap tests, should it be a concern? A quality assurance initiative
Authors: Polanco Jacome Evelyn Carolina , Maerki Jennifer , Chau Karen , Akerman Meredith , Sajjan Sujata , Klein Melissa , Gimenez Cecilia , Laser Alice , Das Kasturi 
Published: May 2018

BackgroundBethesda guidelines do not require presence of transformation zone (TZ) for a cervical Pap test to be deemed adequate. However, clinicians are concerned with specimens that are reported to lack TZ.MethodsWe analyzed 566 ThinPrep cases reported as negative for intraepithelial lesion or malignancy (NILM) with no cervical abnormality detected in previous 4 years (2007–2011). These cases were divided into two cohorts; those with TZ (ETZ) and those without TZ (NTZ). Patients' age, HPV status, time of sample collection (>14 days after last menstrual period), subsequent management, interval of subsequent Pap test (<1, 1–3, and >3 years), and result of subsequent examination were compared over a 5‐year period.ResultsThe rate of abnormal Pap test on 5 year follow‐up was not statistically significant (P < .9520) between cohorts. Our data demonstrates lack of statistical significance between the variables studied. Five year follow‐up of all abnormal Pap smears were analyzed (93% ETZ and 7% NTZ). Of the ETZ group, 25% ASCUS remained as ASCUS and 75% were reported as NILM in subsequent Pap smears. Additionally, 75% of the LSIL were subsequently reported as NILM and 25% reported as ASCUS. One patient reported as HSIL underwent hysterectomy. Two Pap smears performed two years after surgery were negative. Within the NTZ group, one case of ASCUS was NILM upon follow‐up.ConclusionPap smears with NTZ were not at a higher risk for subsequent detection of cervical abnormalities, making earlier repeat testing unnecessary. Rescreening cases without TZ is neither cost effective nor necessary.
The acceptability, feasibility, and possible benefits of a neurobiologically‐informed 5‐day multifamily treatment for adults with anorexia nervosa
Authors: Wierenga Christina E. , Hill Laura , Knatz Peck Stephanie , McCray Jason , Greathouse Laura , Peterson Danika , Scott Amber , Eisler Ivan , Kaye Walter H. 
Published: May 2018

AbstractObjectiveNovel treatments for adults with anorexia nervosa (AN) are lacking. Recent scientific advances have identified neurobiologically‐driven temperament contributors to AN symptoms that may guide development of more effective treatments. This preliminary study evaluates the acceptability, feasibility and possible benefits of a multicenter open trial of an intensive 5‐day neurobiologically‐informed multifamily treatment for adults with AN and their supports (SU). The temperament‐focused treatment combines psychoeducation of AN neurobiology and SU involvement to develop skills to manage traits contributing to disease chronicity.MethodFifty‐four adults with AN and at least one SU (n = 73) received the 5‐day treatment. Acceptability, feasibility, and attrition were measured post‐treatment. Clinical outcome (BMI, eating disorder psychopathology, family function) was assessed post‐treatment and at >3‐month follow‐up.ResultsThe treatment had low attrition, with only one drop‐out. Patients and SU rated the intervention as highly acceptable, and clinicians reported good feasibility. At post‐treatment, patients demonstrated significantly increased BMI, reduced eating disorder psychopathology, and improved family function. Benefits were maintained in the 39 patients who completed follow‐up assessment, with 62% reporting full or partial remission.DiscussionPreliminary results are promising and suggest this novel treatment is feasible and acceptable. To establish treatment efficacy, fully‐powered randomized controlled trials are necessary.
Quantifying natural seasonal variation in mutation parameters with mutation accumulation lines
Authors: Rutter Matthew T. , Roles Angela J. , Fenster Charles B. 
Published: May 2018

AbstractMutations create novel genetic variants, but their contribution to variation in fitness and other phenotypes may depend on environmental conditions. Furthermore, natural environments may be highly heterogeneous. We assessed phenotypes associated with survival and reproductive success in over 30,000 plants representing 100 mutation accumulation lines of Arabidopsis thaliana across four temporal environments at a single field site. In each of the four assays, environmental variance was substantially larger than mutational variance. For some traits, whether mutational variance was significantly varied between seasons. The founder genotype had mean trait values near the mean of the distribution of the mutation accumulation lines in all field experiments. New mutations also contributed more phenotypic variation than would be predicted, given phenotypic and sequence‐level divergence among natural populations of A. thaliana. The combination of large environmental variance with a mean effect of mutation near zero suggests that mutations could contribute substantially to standing genetic variation.