bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chondrogenic differentiation potential of adult and fetal equine cell types
Authors: Adam Emma N. , Janes Jennifer , Lowney Rachael , Lambert Joshua , Thampi Parvathy , Stromberg Arnold , MacLeod James N. 
Published: February 2019

AbstractObjectiveTo determine the chondrogenic potential of cells derived from interzone tissue, the normal progenitor of articular cartilage during fetal development, compared to that of adult bone marrow‐derived and adipose‐derived mesenchymal cell isolates.The objective of this study was to compare the chondrogenic potential of fetal musculoskeletal progenitor cells to adult cell types, which are currently used therapeutically to facilitate joint cartilage repair in equine clinical practice. The hypothesis tested was that cells derived from interzone tissue have a chondrogenic potential that exceeds that of adult bone marrow‐derived and adipose‐derived mesenchymal cell isolates.Study designIn vitro study.AnimalsSix young adult horses (15–17 months of age) and 6 equine fetuses aged 45–46 days of gestation.MethodsThree‐dimensional pellet cultures were established under chondrogenic conditions with fresh, primary cells isolated from adult (articular cartilage, bone marrow, adipose, dermis) and fetal (interzone, skeletal anlagen cartilage, dermis) tissues. Cellular morphology, pellet architecture, and proteoglycan synthesis were assessed in the pellet cultures. Steady state levels of ACAN (aggrecan core protein), COL2A1 (collagen type II), and COL1A1 (collagen type I) messenger RNA (mRNA) were compared among these cell types as pellet cultures and monolayer cultures.ResultsAdult articular chondrocytes, fetal interzone cells, and fetal anlage cells generated the largest pellets under these chondrogenic culture conditions. Pellets derived from adult articular chondrocytes and fetal anlage cells had the highest scores on a neocartilage grading scale. Fetal anlage and adult articular chondrocyte pellets had low steady‐state levels of COL1A mRNA but high COL2A1 expression. Anlage chondrocyte pellets also had the highest expression of ACAN.ConclusionAdult articular chondrocytes, fetal interzone cells, and fetal anlage chondrocytes exhibited the highest chondrogenic potential. In this study, adult adipose‐derived cells exhibited very limited chondrogenesis, and bone marrow‐derived cells had limited and variable chondrogenic potential.Clinical significanceAdditional investigation of the high chondrogenic potential of fetal interzone cells and anlage chondrocytes to advance cell‐based therapies in diarthrodial joints is warranted.
Evaluation of chilling injury and internal browning condition on quality attributes, phenolic content, and antioxidant capacity during sub-optimal cold storage of malaysian cultivar pineapples
Authors: Dolhaji Noer Hartini , Muhamad Ida Idayu , Ya’akub Harisun , Abd Aziz Azila 
Published: February 2019

Pineapple cold storage at sub-optimal temperature (4 ± 2 °C) and duration of storage (0, 7, 14, 21, and 28 days) was periodically tracked to investigate its effect on physical injuries known as chilling injury (CI) and internal browning (IB), which contribute to degradation of pineapple’s quality attributes (i.e., pH, total soluble solids (TSS) concentration, ascorbic acid (AA) content, ion leakage (EL), antioxidant capacity, and total phenolic content (TPC). In this study, three main Malaysian pineapple cultivars were evaluated based on the hypothesis that pineapple’s physical injuries (CI and IB) affect the quality attributes, phenolic content, and antioxidant capacity. Towards day 28 of sub-optimal cold storage treatment, cv. Morris (Queen) showed higher CI and IB incidences. However, the incidences were not statistically significant at a significance level of 0.05 in two other pineapple varieties cv. Josephine (hybrid, between ‘Johor’ Spanish and Smooth Cayenne) and cv. MD-2 or gold (the hybrid of Smooth Cayenne)), which exhibited resistance to CI and IB. Meanwhile, it was found that sub-optimal cold storage up to 28 days affects CI and IB of three main Malaysian pineapple cultivars regardless of their genetic capability towards these injuries. Further, the study revealed that CI and IB symptoms were correlated with a decrease in TSS and pH, and an increase in the percentage of EL, TPC, antioxidant capacity, and antioxidant activities measured by FRAP and DPPH, respectively. Meanwhile, in terms of overall quality attributes result with focus interaction of TPC and antioxidant capacity, cv. MD-2 was observed to be impacted the most during sub-optimal cold storage. The study concluded that CI and IB conditions were positively correlated with the degradation of quality attributes as reflected in TSS content, pH, EL, TPC content, and total antioxidant capacity.
NOX5: Molecular biology and pathophysiology
Authors: Touyz Rhian M. , Anagnostopoulou Aikaterini , Rios Francisco , Montezano Augusto C. , Camargo Livia L. 
Published: February 2019

New Findings
What is the topic of this review?
This review provides a comprehensive overview of Nox5 from basic biology to human disease and highlights unique features of this Nox isoform

What advances does it highlight?
Major advances in Nox5 biology relate to crystallization of the molecule and new insights into the pathophysiological role of Nox5. Recent discoveries have unravelled the crystal structure of Nox5, the first Nox isoform to be crystalized. This provides new opportunities to develop drugs or small molecules targeted to Nox5 in an isoform‐specific manner, possibly for therapeutic use. Moreover genome wide association studies (GWAS) identified Nox5 as a new blood pressure‐associated gene and studies in mice expressing human Nox5 in a cell‐specific manner have provided new information about the (patho) physiological role of Nox5 in the cardiovascular system and kidneys. Nox5 seems to be important in the regulation of vascular contraction and kidney function. In cardiovascular disease and diabetic nephropathy, Nox5 activity is increased and this is associated with increased production of reactive oxygen species and oxidative stress implicated in tissue damage.

AbstractNicotinamide adenine dinucleotide phosphate (NADPH) oxidases (Nox), comprise seven family members (Nox1–Nox5 and dual oxidase 1 and 2) and are major producers of reactive oxygen species in mammalian cells. Reactive oxygen species are crucially involved in cell signalling and function. All Noxs share structural homology comprising six transmembrane domains with two haem‐binding regions and an NADPH‐binding region on the intracellular C‐terminus, whereas their regulatory systems, mechanisms of activation and tissue distribution differ. This explains the diverse function of Noxs. Of the Noxs, NOX5 is unique in that rodents lack the gene, it is regulated by Ca2+, it does not require NADPH oxidase subunits for its activation, and it is not glycosylated. NOX5 localizes in the perinuclear and endoplasmic reticulum regions of cells and traffics to the cell membrane upon activation. It is tightly regulated through numerous post‐translational modifications and is activated by vasoactive agents, growth factors and pro‐inflammatory cytokines. The exact pathophysiological significance of NOX5 remains unclear, but it seems to be important in the physiological regulation of sperm motility, vascular contraction and lymphocyte differentiation, and NOX5 hyperactivation has been implicated in cardiovascular disease, kidney injury and cancer. The field of NOX5 biology is still in its infancy, but with new insights into its biochemistry and cellular regulation, discovery of the NOX5 crystal structure and genome‐wide association studies implicating NOX5 in disease, the time is now ripe to advance NOX5 research. This review provides a comprehensive overview of our current understanding of NOX5, from basic biology to human disease, and highlights the unique characteristics of this enigmatic Nox isoform.
Musculoskeletal ultrasound versus MRI of the hands in healthy subjects – a pilot study
Authors: Micu Mihaela , Bolboaca Sorana D. , Rusu Georgeta M. , Crivii Carmen B. , Solomon Carolina M. 
Published: February 2019

Aim: High resolution imaging methods detect a spectrum of inflammatory-like and structural modifications at joint and tendon level in healthy subjects. The knowledge of their extent and degree is important when subclinical disease activity (implying therapy reassessment) must be differentiated from normality. Musculoskeletal ultrasound (MSUS) evaluation may be challenging even for experts when borderline or low grade lesions are present. Our objective was to analyse the frequency of inflammatory-like lesions in hand joint and tendons in healthy young subjects and to evaluate the concordance between MSUSand magnetic resonance imaging (MRI) findings.Material and methods: Ten healthy young women (age range 24-32 years) clinically asymptomatic (joints and tendons) were selected to have bilateral hand MSUS and MRI evaluation. Based on current definitions, synovitis/tenosynovitis-like lesions, erosions, osteophytes and bone edema were quantified and concordance between the two imaging methods was calculated.Results: Overall, both imaging evaluation methods showed a low frequency of inflammatory-like and structural lesions. No joint presented power Doppler signal or erosions. No abnormalities suggestive for inflammatory or structural pathology were detected at the tendon compartments level. No erosions and no signs of osteitis were detected. The concordance between MSUS and MRI findings was high except for the wrist area.Conclusion: MSUS was demonstrated to be a very accurate imaging method, mostly for hand tendon evaluation. This would allow a better discrimination between normality and pathologic findings, adding supplementary information.
Vitamin D supplementation: are multivitamins sufficient?
Authors: Moon Rebecca J , Curtis Elizabeth M , Cooper Cyrus , Davies Justin H , Harvey Nicholas C 
Published: February 2019

BackgroundPublic Health England advises 400 IU/day vitamin D supplementation for children over 1 year. Commercially available children’s multivitamin and vitamin D supplements were surveyed to determine the vitamin D content.MethodsMultivitamins and vitamin D supplements marketed at children <12 years and sold by nine UK supermarkets and health supplement retailers were surveyed. Vitamin D content was determined from manufacturer’s websites and product packaging.Results67 multivitamins were surveyed, containing 0–800 IU/day vitamin D. Only 25%–36%, depending on the child’s age, provided ≥400 IU/day vitamin D. Supplements containing only vitamin D or labelled as for ‘healthy bones’ typically had higher vitamin D content (57%–67% contained ≥400 IU/day).ConclusionsFew multivitamin products supply the recommended 400 IU/day vitamin D. Clinicians need to be aware of this when recommending vitamin D supplementation and advise parents/carers to choose a product that contains ≥400 IU/day vitamin D.
Including the Nordic hamstring exercise in injury prevention programmes halves the rate of hamstring injuries: a systematic review and meta-analysis of 8459 athletes
Authors: van Dyk Nicol , Behan Fearghal P , Whiteley Rod 
Published: February 2019

Research questionDoes the Nordic hamstring exercise (NHE) prevent hamstring injuries when included as part of an injury prevention intervention?DesignSystematic review and meta-analysis.Eligibility criteria for selecting studiesWe considered the population to be any athletes participating in any sporting activity, the intervention to be the NHE, the comparison to be usual training or other prevention programmes, which did not include the NHE, and the outcome to be the incidence or rate of hamstring injuries.AnalysisThe effect of including the NHE in injury prevention programmes compared with controls on hamstring injuries was assessed in 15 studies that reported the incidence across different sports and age groups in both women and men.Data sourcesMEDLINE via PubMed, CINAHL via Ebsco, and OpenGrey.ResultsThere is a reduction in the overall injury risk ratio of 0.49 (95% CI 0.32 to 0.74, p=0.0008) in favour of programmes including the NHE. Secondary analyses when pooling the eight randomised control studies demonstrated a small increase in the overall injury risk ratio 0.52 (95% CI 0.32 to 0.85, p=0.0008), still in favour of the NHE. Additionally, when studies with a high risk of bias were removed (n=8), there is an increase of 0.06 in the risk ratio to 0.55 (95% CI 0.34 to 0.89, p=0.006).ConclusionsProgrammes that include the NHE reduce hamstring injuries by up to 51%. The NHE essentially halves the rate of hamstring injuries across multiple sports in different athletes.Trial registration numberPROSPERO CRD42018106150.
Combined education and patient-led goal setting intervention reduced chronic low back pain disability and intensity at 12 months: a randomised controlled trial
Authors: Gardner Tania , Refshauge Kathryn , McAuley James , Hübscher Markus , Goodall Stephen , Smith Lorraine 
Published: February 2019

BackgroundOne model of care that has not been tested for chronic low back pain (LBP) is patient-led goal setting. We aimed to compare the clinical effectiveness and healthcare use of a patient-led goal setting approach (intervention) with simple advice to exercise (control) over 12 months.MethodsAn assessor-blinded randomised controlled trial. Intervention was education combined with patient-led goal setting compared with a control group receiving a standardised exercise programme. The primary outcomes were back pain disability and pain intensity. Secondary outcomes were quality of life, kinesiophobia, self-efficacy, depression, anxiety and stress. Outcomes and healthcare use were assessed immediately post-treatment (2 months) and after 4 and 12 months. Analysis was by intention to treat.ResultsSeventy-five patients were randomly assigned to either the intervention (n=37) or the control (n=38) group. Using linear mixed model analyses, adjusted mean changes in primary outcomes of disability and pain intensity were greater in the intervention group than in the control group (disability post-treatment: p<0.05). These differences were clinically meaningful. Mean differences in all secondary measures were greater in the intervention group than in the control group (p<0.05). There was no difference in healthcare use between groups over 12 months.ConclusionA patient-led goal setting intervention was significantly more effective than advice to exercise for improving outcomes in disability, pain intensity, quality of life, self-efficacy and kinesiophobia in chronic LBP. These improvements were maintained at 12 months. Smaller effects were seen in measures of depression, anxiety and stress.Trial registration numberACTRN12614000830695.
Resilience in inpatient palliative care nursing: a qualitative systematic review
Authors: Powell Martin J , Froggatt Katherine , Giga Sabir 
Published: February 2019

BackgroundNurses in inpatient palliative care are frequently exposed to death and dying in addition to common stressors found in other nursing practice. Resilience may mitigate against stress but remains ill-defined and under-researched in the specialist palliative care setting.ObjectiveThe aim of this systematic review was to understand resilience from the perspectives of inpatient palliative care nurses.DesignA thematic synthesis of qualitative studies was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.Data sourcesAcademic Search Ultimate, Cumulative Index to Nursing and Allied Health Literature, Medline Complete, PsycINFO and Scopus.Review methodsThe review stages were searching for relevant literature, selecting relevant papers, data extraction, critical appraisal and thematic synthesis.ResultsEight studies revealed 10 subthemes, 3 descriptive themes and 1 analytical theme: resilience occurs when nurses incorporate stressful aspects of their personal or professional lives into a coherent narrative that enhances their ability to cope with the demands of their role.ConclusionPalliative care nursing is more stressful if patients or situations remind nurses of personal experiences. Nurses cope better with adequate support; however, coping does not necessarily imply increased resilience. Resilience occurs when nurses cognitively process their experiences, articulate their thoughts and feelings into a coherent narrative, and construct a sense of meaning or purpose. Future research could explore how nurses understand resilience and how it could be enhanced in the palliative care inpatient setting. With resilience, nurses may remain in the profession longer and improve the quality of care when they do.
Long work hours, weekend working and depressive symptoms in men and women: findings from a UK population-based study
Authors: Weston Gillian , Zilanawala Afshin , Webb Elizabeth , Carvalho Livia A , McMunn Anne 
Published: February 2019

BackgroundGlobalised and 24/7 business operations have fuelled demands for people to work long hours and weekends. Research on the mental health effects of these intensive temporal work patterns is sparse, contradictory or has not considered gender differences. Our objective was to examine the relationship between these work patterns and depressive symptoms in a large nationally representative sample of working men and women in the UK.MethodThe current study analysed data from Understanding Society, the UK Household Longitudinal Study, of 11 215 men and 12 188 women in employment or self-employment at the time of the study. Ordinary least squares regression models, adjusted for potential confounders and psychosocial work factors, were used to estimate depressive symptoms across categories of work hours and weekend work patterns.ResultsRelative to a standard 35–40 hours/week, working 55 hours/week or more related to more depressive symptoms among women (ß=0.75, 95% CI 0.12 to 1.39), but not for men (ß=0.24, 95% CI −0.10 to 0.58). Compared with not working weekends, working most or all weekends related to more depressive symptoms for both men (ß=0.34, 95% CI 0.08 to 0.61) and women (ß=0.50, 95% CI 0.20 to 0.79); however, working some weekends only related to more depressive symptoms for men (ß=0.33, 95% CI 0.11 to 0.55), not women (ß=0.17, 95% CI −0.09 to 0.42).ConclusionIncreased depressive symptoms were independently linked to working extra-long hours for women, whereas increased depressive symptoms were associated with working weekends for both genders, suggesting these work patterns may contribute to worse mental health.
Diversity, structure and natural history of amphibians in the upper Claro River basin, a buffer zone of the National Natural Park Los Nevados, Central Cordillera of Colombia
Authors: Rojas-Morales Julián Andrés , Marín-Martínez Mateo 
Published: February 2019

We present an assessment on composition, diversity and structure of amphibians in three zones along an elevation gradient (2,400–3,000 m) in the Central Cordillera of Colombia. For this purpose, we carried out two field trips in November 2014 and February 2015, covering rainy and dry seasons, respectively. Diurnal (08:00–12:00 h) and nocturnal (18:00–22:00 h) visual encounter surveys were made without spatial restrictions. The diversity for each zone (alpha) and for the entire landscape (gamma) was evaluated by the effective number of species, and the structure of the communities was analyzed by range-abundance curves. The inequality factor for each of the sampling zones was also calculated. A total of 15 species belonging to seven genera and three families were recorded, all of the order Anura. Craugastoridae with 11 species (73.3% of richness) and Pristimantis (eight species) were the most diverse family and genus, respectively. The average alpha diversity per zone was 6.6 effective species, with zone A being the most diverse with eight species. In terms of beta diversity we found 2.5 effective communities at the landscape level, and differences between zones are given by the rare species, while the most abundant ones (e.g., Pristimantis uranobates) are shared between them. It is presumed that the greater diversity of zone A is due to the lower elevation and better state of conservation if compared to the other two zones. Of the total species recorded, three are threatened with extinction: Endangered (Hypodactylus latens, Osornophryne percrassa), and Critically Endangered (Niceforonia adenobrachia). The finding of three yet undescribed species is highlighted.
Rediscovery of the ‘extinct’ bee Hesperocolletes douglasi Michener, 1965 (Colletidae: Colletinae: Paracolletini) in Western Australia and first description of the female
Authors: Arnold Juliana Pille , Murphy Mark V. , Didham Raphael K. , Houston Terry F. 
Published: February 2019

The second known specimen of the bee Hesperocolletes douglasi Michener, 1965 is here reported as a serendipitous find among a collection of insect pollinators from an isolated woodland remnant in the Southwest Floristic Region of Western Australia. The unique male holotype of this monotypic genus of bees was collected 80 years ago and officially gazetted as presumed extinct in 1994. With our collection of a female specimen in 2015, however, it now appears that H. douglasi may persist as an extant localised population. Follow-up efforts to find more specimens at the collection locality so far proved unsuccessful, indicating that the species is likely either very rare or inhabits an ecological niche that is yet to be discovered. Analysis of the pollen load carried by the female indicates that the species may be polylectic. We discuss the context of the rediscovery of the bee, provide a detailed description and illustrations of the female, and make observations about the unusual morphological characteristics of the species. The rediscovery of H. douglasi emphasizes the importance of conservation efforts for remnant woodlands in the region, both as potential habitat for the bee and as remaining habitat essential for other rare and threatened species in this global biodiversity hotspot.
DASH Dietary Pattern, Mediation by Mineral Intakes, and the Risk of Coronary Artery Disease and Stroke Mortality
Authors: Talaei Mohammad , Koh Woon‐Puay , Yuan Jian‐Min , van Dam Rob M. 
Published: February 2019

Background

The association of the Dietary Approaches to Stop Hypertension (
DASH
) dietary pattern with stroke and coronary artery disease (
CAD
) mortality has not been evaluated in Asian populations, and the role of mineral intakes as potential mediators is not clear.

Methods and Results

We used data from 57 078 participants of the Singapore Chinese Health Study aged 45 to 74 years at baseline (1993–1998). Information on usual diet was collected by a validated 165‐item food frequency questionnaire at recruitment, and mortality information was obtained via registry linkage up to December 31, 2014. We constructed
DASH
scores based on quintiles of intake of 7 predefined food items and sodium. Cox proportional hazard models were used to calculate hazard ratios and corresponding 95% CIs. Greater adherence to the
DASH
dietary pattern was significantly associated with a lower risk of
CAD
(hazard ratio between extreme quintiles, 0.76; 95%
CI
, 0.65–0.90;
P
trend<0.001) and stroke (hazard ratio, 0.62; 95%
CI
, 0.50–0.78;
P
trend<0.001) mortality. We found an inverse association between potassium intake and
CAD
mortality and a direct association between sodium intake and stroke mortality. No other significant associations were observed for potassium, sodium, magnesium, and calcium intakes in relation to
CAD
or stroke mortality. Adjustment for mineral intakes did not materially change the association of the
DASH
score with
CAD
or stroke mortality.

Conclusions

Adherence to the
DASH
dietary pattern was associated with substantially lower risk of
CAD
and stroke mortality in an Asian population, and this inverse association did not appear to be substantially mediated by intakes of sodium, potassium, magnesium, and calcium.