bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Macaca radiata as a non-human primate model of Dengue virus infection
Authors: Kato Fumihiro , Ishida Yuki , Kawakami Akihiko , Takasaki Tomohiko , Saijo Masayuki , Miura Tomoyuki , Hishiki Takayuki 
Published: February 2018

AbstractDengue virus (DENV) causes a wide range of illnesses in humans, including dengue fever and dengue haemorrhagic fever. Current animal models of DENV infection are limited for understanding infectious diseases in humans. Bonnet monkeys (Macaca radiata), a type of Old World monkey, have been used to study experimental and natural infections by flaviviruses, but Old World monkeys have not yet been used as DENV infection models. In this study, the replication levels of several DENV strains were evaluated using peripheral blood mononuclear cells. Our findings indicated that DENV-4 09-48 strain, isolated from a traveller returning from India in 2009, was a highly replicative virus. Three bonnet monkeys were infected with 09-48 strain and antibody responses were assessed. DENV nonstructural protein 1 antigen was detected and high viraemia was observed. These results indicated that bonnet monkeys and 09-48 strain could be used as a reliable primate model for the study of DENV.
Early Detection of Preeclampsia Using Circulating Small non-coding RNA
Authors: Yoffe Liron , Gilam Avital , Yaron Orly , Polsky Avital , Farberov Luba , Syngelaki Argyro , Nicolaides Kypros , Hod Moshe , Shomron Noam 
Published: February 2018

AbstractPreeclampsia is one of the most dangerous pregnancy complications, and the leading cause of maternal and perinatal mortality and morbidity. Although the clinical symptoms appear late, its origin is early, and hence detection is feasible already at the first trimester. In the current study, we investigated the abundance of circulating small non-coding RNAs in the plasma of pregnant women in their first trimester, seeking transcripts that best separate the preeclampsia samples from those of healthy pregnant women. To this end, we performed small non-coding RNAs sequencing of 75 preeclampsia and control samples, and identified 25 transcripts that were differentially expressed between preeclampsia and the control groups. Furthermore, we utilized those transcripts and created a pipeline for a supervised classification of preeclampsia. Our pipeline generates a logistic regression model using a 5-fold cross validation on numerous random partitions into training and blind test sets. Using this classification procedure, we achieved an average AUC value of 0.86. These findings suggest the predictive value of circulating small non-coding RNA in the first trimester, warranting further examination, and lay the foundation for producing a novel early non-invasive diagnostic tool for preeclampsia, which could reduce the life-threatening risk for both the mother and fetus.
Fabrication of transparent hemispherical 3D nanofibrous scaffolds with radially aligned patterns via a novel electrospinning method
Authors: Kim Jeong In , Kim Ju Yeon , Park Chan Hee 
Published: February 2018

AbstractTissue engineering has significantly contributed to the development of optimal treatments for individual injury sites based on their unique functional and histologic properties. Human organs and tissue have three-dimensional (3D) morphologies; for example, the morphology of the eye is a spherical shape. However, most conventional electrospinning equipment is only capable of fabricating a two-dimensional (2D) structured fibrous scaffold and no report is available on a 3D electrospinning method to fabricate a hemispherical scaffold to mimic the native properties of the cornea, including microscopic to macroscopic morphology and transparency. We proposed a novel electrospinning method using a single nonconductive hemispherical device and a metal pin. A designed peg-top shaped collector, a hemispherical nonconductive device with a metal pin in the center and copper wire forming a circle around at the edge was attached to a conventional conductive collector. A 3D hemispherical transparent scaffold with radially aligned nanofibers was successfully fabricated with the designed peg-top collector. In summary, our fabricated 3D electrospun scaffold is expected to be suitable for the treatment of injuries of ocular tissues owing to the hemispherical shape and radially aligned nanofibers which can guide the direction of the main collagen and cellular actin filament in the extracellular matrix.
Spin canting across core/shell Fe3O4/MnxFe3−xO4 nanoparticles
Authors: Oberdick Samuel D. , Abdelgawad Ahmed , Moya Carlos , Mesbahi-Vasey Samaneh , Kepaptsoglou Demie , Lazarov Vlado K. , Evans Richard F. L. , Meilak Daniel , Skoropata Elizabeth , van Lierop Johan , Hunt-Isaak Ian , Pan Hillary , Ijiri Yumi , Krycka Kathryn L. , Borchers Julie A. , Majetich Sara A. 
Published: February 2018

AbstractMagnetic nanoparticles (MNPs) have become increasingly important in biomedical applications like magnetic imaging and hyperthermia based cancer treatment. Understanding their magnetic spin configurations is important for optimizing these applications. The measured magnetization of MNPs can be significantly lower than bulk counterparts, often due to canted spins. This has previously been presumed to be a surface effect, where reduced exchange allows spins closest to the nanoparticle surface to deviate locally from collinear structures. We demonstrate that intraparticle effects can induce spin canting throughout a MNP via the Dzyaloshinskii-Moriya interaction (DMI). We study ~7.4 nm diameter, core/shell Fe3O4/MnxFe3−xO4 MNPs with a 0.5 nm Mn-ferrite shell. Mössbauer spectroscopy, x-ray absorption spectroscopy and x-ray magnetic circular dichroism are used to determine chemical structure of core and shell. Polarized small angle neutron scattering shows parallel and perpendicular magnetic correlations, suggesting multiparticle coherent spin canting in an applied field. Atomistic simulations reveal the underlying mechanism of the observed spin canting. These show that strong DMI can lead to magnetic frustration within the shell and cause canting of the net particle moment. These results illuminate how core/shell nanoparticle systems can be engineered for spin canting across the whole of the particle, rather than solely at the surface.
Representations of naturalistic stimulus complexity in early and associative visual and auditory cortices
Authors: Güçlütürk Yağmur , Güçlü Umut , van Gerven Marcel , van Lier Rob 
Published: February 2018

AbstractThe complexity of sensory stimuli has an important role in perception and cognition. However, its neural representation is not well understood. Here, we characterize the representations of naturalistic visual and auditory stimulus complexity in early and associative visual and auditory cortices. This is realized by means of encoding and decoding analyses of two fMRI datasets in the visual and auditory modalities. Our results implicate most early and some associative sensory areas in representing the complexity of naturalistic sensory stimuli. For example, parahippocampal place area, which was previously shown to represent scene features, is shown to also represent scene complexity. Similarly, posterior regions of superior temporal gyrus and superior temporal sulcus, which were previously shown to represent syntactic (language) complexity, are shown to also represent music (auditory) complexity. Furthermore, our results suggest the existence of gradients in sensitivity to naturalistic sensory stimulus complexity in these areas.
Tubulation repair mitigates misdirection of regenerating motor axons across a sciatic nerve gap in rats
Authors: Liu Dan , Mi Daguo , Zhang Tuanjie , Zhang Yanping , Yan Junying , Wang Yaxian , Tan Xuefeng , Yuan Ying , Yang Yumin , Gu Xiaosong , Hu Wen 
Published: February 2018

AbstractThe repair of peripheral nerve laceration injury to obtain optimal function recovery remains a big challenge in the clinic. Misdirection of regenerating axons to inappropriate target, as a result of forced mismatch of endoneurial sheaths in the case of end-to-end nerve anastomosis or nerve autografting, represents one major drawback that limits nerve function recovery. Here we tested whether tubulation repair of a nerve defect could be beneficial in terms of nerve regeneration accuracy and nerve function. We employed sequential retrograde neuronal tracing to assess the accuracy of motor axon regeneration into the tibial nerve after sciatic nerve laceration and entubulation in adult Sprague-Dawley rats. In a separate cohort of rats with the same sciatic nerve injury/repair protocols, we evaluated nerve function recovery behaviorally and electrophysiologically. The results showed that tubulation repair of the lacerated sciatic nerve using a 3-6-mm-long bioabsorbable guidance conduit significantly reduced the misdirection of motor axons into the tibial nerve as compared to nerve autografting. In addition, tubulation repair ameliorated chronic flexion contracture. This study suggests that tubulation repair of a nerve laceration injury by utilizing a bioresorbable nerve guidance conduit represents a potential substitute for end-to-end epineurial suturing and nerve autografting.
Synthetic dimensions in ultracold polar molecules
Authors: Sundar Bhuvanesh , Gadway Bryce , Hazzard Kaden R. A. 
Published: February 2018

AbstractSynthetic dimensions alter one of the most fundamental properties in nature, the dimension of space. They allow, for example, a real three-dimensional system to act as effectively four-dimensional. Driven by such possibilities, synthetic dimensions have been engineered in ongoing experiments with ultracold matter. We show that rotational states of ultracold molecules can be used as synthetic dimensions extending to many – potentially hundreds of – synthetic lattice sites. Microwaves coupling rotational states drive fully controllable synthetic inter-site tunnelings, enabling, for example, topological band structures. Interactions leads to even richer behavior: when molecules are frozen in a real space lattice with uniform synthetic tunnelings, dipole interactions cause the molecules to aggregate to a narrow strip in the synthetic direction beyond a critical interaction strength, resulting in a quantum string or a membrane, with an emergent condensate that lives on this string or membrane. All these phases can be detected using local measurements of rotational state populations.
Development of a model predicting non-satisfaction 1 year after primary total knee replacement in the UK and transportation to Switzerland
Authors: Garriga Cesar , Sanchez-Santos Maria T. , Judge Andrew , Perneger Thomas , Hannouche Didier , Lübbeke Anne , Arden Nigel K. 
Published: February 2018

AbstractWe aimed to develop a predictive model for non-satisfaction following primary total knee replacement (TKR) and to assess its transportability to another health care system. Data for model development were obtained from two UK tertiary hospitals. Model transportation data were collected from Geneva University Hospitals in Switzerland. Participants were individuals undergoing primary TKR with non-satisfaction with surgery after one year the outcome of interest. Multiple imputation and logistic regression modelling with bootstrap backward selection were used to identify predictors of outcome. Model performance was assessed by discrimination and calibration. 64 (14.2%) patients in the UK and 157 (19.9%) in Geneva were non-satisfied with their TKR. Predictors in the UK cohort were worse pre-operative pain and function, current smoking, treatment for anxiety and not having been treated with injected corticosteroids (corrected AUC = 0.65). Transportation to the Geneva cohort showed an AUC of 0.55. Importantly, two UK predictors (treated for anxiety, injected corticosteroids) were not predictive in Geneva. A better model fit was obtained when coefficients were re-estimated in the Geneva sample (AUC = 0.64). The model did not perform well when transported to a different country, but improved when it was re-estimated. This emphasises the need to re-validate the model for each setting/country.
Development and validation of a clinical prediction model for patient-reported pain and function after primary total knee replacement surgery
Authors: Sanchez-Santos M. T. , Garriga C. , Judge A. , Batra R. N. , Price A. J. , Liddle A. D. , Javaid M. K. , Cooper C. , Murray D. W. , Arden N. K. 
Published: February 2018

AbstractTo develop and validate a clinical prediction model of patient-reported pain and function after undergoing total knee replacement (TKR). We used data of 1,649 patients from the Knee Arthroplasty Trial who received primary TKR across 34 centres in the UK. The external validation included 595 patients from Southampton University Hospital, and Nuffield Orthopaedic Centre (Oxford). The outcome was the Oxford Knee Score (OKS) 12-month after TKR. Pre-operative predictors including patient characteristics and clinical factors were considered. Bootstrap backward linear regression analysis was used. Low pre-operative OKS, living in poor areas, high body mass index, and patient-reported anxiety or depression were associated with worse outcome. The clinical factors associated with worse outcome were worse pre-operative physical status, presence of other conditions affecting mobility and previous knee arthroscopy. Presence of fixed flexion deformity and an absent or damaged pre-operative anterior cruciate ligament (compared with intact) were associated with better outcome. Discrimination and calibration statistics were satisfactory. External validation predicted 21.1% of the variance of outcome. This is the first clinical prediction model for predicting self-reported pain and function 12 months after TKR to be externally validated. It will help to inform to patients regarding expectations of the outcome after knee replacement surgery.
Neuronal avalanche dynamics indicates different universality classes in neuronal cultures
Authors: Yaghoubi Mohammad , de Graaf Ty , Orlandi Javier G. , Girotto Fernando , Colicos Michael A. , Davidsen Jörn 
Published: February 2018

AbstractNeuronal avalanches have become an ubiquitous tool to describe the activity of large neuronal assemblies. The emergence of scale-free statistics with well-defined exponents has led to the belief that the brain might operate near a critical point. Yet not much is known in terms of how the different exponents arise or how robust they are. Using calcium imaging recordings of dissociated neuronal cultures we show that the exponents are not universal, and that significantly different exponents arise with different culture preparations, leading to the existence of different universality classes. Naturally developing cultures show avalanche statistics consistent with those of a mean-field branching process, however, cultures grown in the presence of folic acid metabolites appear to be in a distinct universality class with significantly different critical exponents. Given the increased synaptic density and number of feedback loops in folate reared cultures, our results suggest that network topology plays a leading role in shaping the avalanche dynamics. We also show that for both types of cultures pronounced correlations exist in the sizes of neuronal avalanches indicating size clustering, being much stronger in folate reared cultures.
A Simple 3-Parameter Model for Cancer Incidences
Authors: Zhang Xiaoxiao , Fröhlich Holger , Grigoriev Dima , Vakulenko Sergey , Zimmermann Jörg , Weber Andreas Günter 
Published: February 2018

AbstractWe propose a simple 3-parameter model that provides very good fits for incidence curves of 18 common solid cancers even when variations due to different locations, races, or periods are taken into account. From a data perspective, we use model selection (Akaike information criterion) to show that this model, which is based on the Weibull distribution, outperforms other simple models like the Gamma distribution. From a modeling perspective, the Weibull distribution can be justified as modeling the accumulation of driver events, which establishes a link to stem cell division based cancer development models and a connection to a recursion formula for intrinsic cancer risk published by Wu et al. For the recursion formula a closed form solution is given, which will help to simplify future analyses. Additionally, we perform a sensitivity analysis for the parameters, showing that two of the three parameters can vary over several orders of magnitude. However, the shape parameter of the Weibull distribution, which corresponds to the number of driver mutations required for cancer onset, can be robustly estimated from epidemiological data.
Changes in functional brain network topology after successful and unsuccessful corpus callosotomy for Lennox-Gastaut Syndrome
Authors: Liang Jun-Ge , Kim Nam-Young , Ko Ara , Kim Heung Dong , Lee Dongpyo 
Published: February 2018

AbstractCorpus callosotomy (CC) is an effective palliative surgical treatment for patients with Lennox-Gastaut Syndrome (LGS). However, research on the long-term functional effects of CC is sparse. We aimed to investigate these effects and their associated clinical conditions over the two years after CC. Long-term clinical EEG recordings of 30 patients with LGS who had good and bad seizure outcome after CC were collected and retrospectively studied. It was found that CC caused brain network ‘hubs’ to shift from paramedian to lateral regions in the good-recovery group, which reorganized the brain network into a more homogeneous state. We also found increased local clustering coefficients in patients with bad outcomes and decreases, implying enhanced network integration, in patients with good outcomes. The small worldness of brain networks in patients with good outcomes increased in the two years after CC, whereas it decreased in patients with bad outcomes. The covariation of small-worldness with the rate of reduction in seizure frequency suggests that this can be used as an indicator of CC outcome. Local and global network changes during the long-term state might be associated with the postoperative recovery process and could serve as indicators for CC outcome and long-term LGS recovery.
Optimized Bone Regeneration in Calvarial Bone Defect Based on Biodegradation-Tailoring Dual-shell Biphasic Bioactive Ceramic Microspheres
Authors: Xu Antian , Zhuang Chen , Xu Shuxin , He Fuming , Xie Lijun , Yang Xianyan , Gou Zhongru 
Published: February 2018

AbstractBioceramic particulates capable of filling bone defects have gained considerable interest over the last decade. Herein, dual-shell bioceramic microspheres (CaP@CaSi@CaP, CaSi@CaP@CaSi) with adjustable beta-tricalcium phosphate (CaP) and beta-calcium silicate (CaSi) distribution were fabricated using a co-concentric capillary system enabling bone repair via a tailorable biodegradation process. The in vitro results showed the optimal concentration (1/16 of 200 mg/ml) of extracts of dual-shell microspheres could promote bone marrow mesenchymal cell (BMSC) proliferation and enhance the level of ALP activity and Alizarin Red staining. The in vivo bone repair and microsphere biodegradation in calvarial bone defects were compared using micro-computed tomography and histological evaluations. The results indicated the pure CaP microspheres were minimally resorbed at 18 weeks post-operatively and new bone tissue was limited; however, the dual-shell microspheres were appreciably biodegraded with time in accordance with the priority from CaSi to CaP in specific layers. The CaSi@CaP@CaSi group showed a significantly higher ability to promote bone regeneration than the CaP@CaSi@CaP group. This study indicates that the biphasic microspheres with adjustable composition distribution are promising for tailoring material degradation and bone regeneration rate, and such versatile design strategy is thought to fabricate various advanced biomaterials with tailorable biological performances for bone reconstruction.
Visualisation of DCP, a nerve agent mimic, in Catfish brain by a simple chemosensor
Authors: Sarkar Himadri Sekhar , Ghosh Ayndrila , Das Sujoy , Maiti Pulak Kumar , Maitra Sudipta , Mandal Sukhendu , Sahoo Prithidipa 
Published: February 2018

AbstractA chemosensor, 3-aminophenol-based rhodamine conjugate (ARC) has been developed for visualisation of diethylchlorophosphate (DCP), mimic of a chemical warfare agent, in Catfish brain. The simple detection of DCP by “turn-on” fluorescence property of the chemosensor makes it unique for easy and rapid in vivo and in vitro detection of DCP with the detection limit of 5.6 nM.
Trends in seasonal warm anomalies across the contiguous United States: Contributions from natural climate variability
Authors: Yu Lejiang , Zhong Shiyuan , Heilman Warren E. , Bian Xindi 
Published: February 2018

AbstractMany studies have shown the importance of anthropogenic greenhouse gas emissions in contributing to observed upward trends in the occurrences of temperature extremes over the U.S. However, few studies have investigated the contributions of internal variability in the climate system to these observed trends. Here we use daily maximum temperature time series from the North American Land Data Assimilation System Phase 2 (NLDAS-2) dataset to identify trends in seasonal warm anomalies over the contiguous U.S. in the three most recent decades and explore their relationships to low-frequency modes of internal climate variability. The results reveal substantial upward trends in the frequency of warm anomalies in all seasons and in all regions of the U.S., except for portions of the Intermountain West in winter where significant downward trends occur. The strengths and regional coverage of the trends, however, differ considerably by season. These trends can be explained, in part, by the large-scale anomalous atmospheric circulations associated with low-frequency sea-surface temperature oscillations characterized by the Pacific Decadal Oscillation (PDO) and the Atlantic Multidecadal Oscillation (AMO). The association between the upward trends in the seasonal warm anomalies and PDO and AMO is further confirmed by the century-long (1871–2012) Twentieth Century Reanalysis dataset.