bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treatment with tofacitinib attenuates muscle loss through myogenin activation in the collagen-induced arthritis
Authors: da Rosa Thales Hein , Bartikoski Bárbara Jonson , do Espírito Santo Rafaela Cavalheiro , Farinon Mirian , de Souza Silva Jordana Miranda , Pedó Renata Ternus , Gasparini Maria Luísa , Karnopp Thais , dos Santos Leonardo Peterson , Chapacais Gustavo , di Domenico Andressa , Loch Sofia , Xavier Ricardo Machado 
Published: November 2024

Abstract
Background
Sarcopenia is a muscle disease characterized by reduction of muscle strength and muscle mass. In RA, 25.9 to 43.3% of the patients present sarcopenia. The loss of muscle mass observed in RA patients occurs either by activation of catabolic pathways or by inhibition of anabolic pathways. Despite having a list of drugs capable of treating RA inflammation, their effect on muscle is unclear. Our objective was to evaluate the tofacitinib effect on the muscle mass of collagen-induced arthritis (CIA) mice.

Methods
CIA was induced in male DBA/1J mice by subcutaneous injection of Type 2 Collagen plus Freund Adjuvant. Animals were randomized into 3 groups: CIA + tofacitinib; CIA + vehicle; and healthy controls. Treatment was administered twice a day, between days 18 and 45 after induction. Clinical score, edema, and body weight were evaluated during the experimental period. After euthanasia, tibiotarsal joints were collected for assessment of disease histopathological score, and tibialis anterior (TA) and gastrocnemius (GA) muscles were weighed to assess muscle mass. Muscle atrophy was evaluated by measurement of TA myofiber cross-sectional area (CSA). Protein expression was evaluated by western blot using GA homogenates. Serum inflammatory markers were evaluated by ELISA. Statistical analysis included ANOVA followed by Tukey’s or with Kruskal-Wallis. The statistical difference was assumed for p < 0.05.

Results
Tofacitinib treatment decreased arthritis severity by reducing clinical score, and hind paw edema in comparison with the vehicle group. Tofacitinib showed weight gain, higher TA and GA weights, and increased CSA compared to the vehicle group. On day 45, Tofacitinib presented increased muscle strength compared to the vehicle group, however, no difference was found in muscle fatigue. Pax7 expression was unchanged, while MyoD expression showed an increasing trend, and myogenin expression was significantly increased in Tofacitinib compared to vehicle and control groups. The treatment didn’t modify Murf-1 expression. Tofacitinib mice showed decreased serum levels of TNF and increased IL-6 serum levels.

Conclusion
Tofacitinib attenuated muscle loss in arthritic mice, increased muscle weight and muscle CSA. Activation of satellite cell regeneration, based on the increased expression of myogenin, is a potential mechanism involved in tofacitinib action against muscle loss.
QTc interval prolongation impact on in-hospital mortality in acute coronary syndromes patients using artificial intelligence and machine learning
Authors: El Amrawy Ahmed Mahmoud , Abd El Salam Samar Fakhr El Deen , Ayad Sherif Wagdy , Sobhy Mohamed Ahmed , Awad Aya Mohamed 
Published: November 2024

Abstract
Background
Prediction of mortality in hospitalized patients is a crucial and important problem. Several severity scoring systems over the past few decades and machine learning models for mortality prediction have been developed to predict in-hospital mortality. Our aim in this study was to apply machine learning (ML) algorithms using QTc interval to predict in-hospital mortality in ACS patients and compare them to the validated conventional risk scores.

Results
This study was retrospective, using supervised learning, and data mining. Out of a cohort of 500 patients admitted to a tertiary care hospital from September 2018 to August 2020, who presented with ACS. Prediction models for in-hospital mortality in ACS patients were developed using 3 ML algorithms. We employed the ensemble learning random forest (RF) model, the Naive Bayes (NB) model and the rule-based projective adaptive resonance theory (PART) model. These models were compared to one another and to two conventional validated risk scores; the Global Registry of Acute Coronary Events (GRACE) risk score and Thrombolysis in Myocardial Infarction (TIMI) risk score. Out of the 500 patients included in our study, 164 (32.8%) patients presented with unstable angina, 148 (29.6%) patients with non-ST-elevation myocardial infarction (NSTEMI) and 188 (37.6%) patients were having ST-elevation myocardial infarction (STEMI). 64 (12.8%) patients died in-hospital and the rest survived. Performance of prediction models was measured in an area under the receiver operating characteristic curve (AUC) ranged from 0.83 to 0.93 using all available variables compared to the GRACE score (0.9 SD 0.05) and the TIMI score (0.75 SD 0.02). Using QTc as a stand-alone variable yielded (0.67 SD 0.02) with a cutoff value 450 using Bazett’s formula, whereas using QTc in addition to other variables of personal and clinical data and other ECG variables, the result was 0.8 SD 0.04. Results of RF and NB models were almost the same, but PART model yielded the least results. There was no significant difference of AUC values after replacing the missing values and applying class balancer.

Conclusions
The proposed method can effectively predict patients at high risk of in-hospital mortality early in the setting of ACS using only clinical and ECG data. Prolonged QTc interval can be used as a risk predictor of in-hospital mortality in ACS patients.
Performance of complementor’s complementary goods on platform ecosystems: the perspective of individual complementors' complementary goods development behavior
Authors: Zhou Xiaoxiao , Inoue Yuki 
Published: November 2024

AbstractThe purpose of our study was to examine how individual complementors' development behaviors (specifically, diversity- and distinctiveness-oriented development behaviors) impact the performance of complementary goods on the platform. To achieve this, we employed fixed-effects regression analysis and utilized panel data obtained from the free music platform “DOVA.” This study examines two aspects of the performance of complementor’s complementary goods: the highest performance related to the success of the platform ecosystem and the lowest performance related to platform ecosystem sustainment. The findings indicate that a high focus on diversity-oriented development behavior is positively correlated with the complementor's highest performance and negatively correlated with complementor's lowest performance. Additionally, distinctiveness-oriented development behavior also positively correlated with complementor's highest performance. However, low distinctiveness-oriented development behavior is negatively correlated with complementor's lowest performance. We reveal desirable development behaviors for complementary goods related to both content innovation and platform ecosystem sustainability. Based on these understandings, platform owners can target platform governance.
Physiotherapists’ knowledge, attitude and practice of clinical prediction rules in low-back pain
Authors: Mbada Chidozie Emmanuel , Afolabi Victor Adeboye , Oghumu Saturday N. , Gebrye Tadesse , Ademoyegun Adekola , Adje Mishael , Karstens Sven , Fatoye Francis 
Published: November 2024

Abstract
Background and aim
Clinical prediction rules (CPRs) are mathematical tools that are intended to guide clinicians in clinical decision making or predict a future outcome, but they seem rather unknown, under-utilized, or avoided by clinicians. This study aimed to assess knowledge, attitude, and practice of CPRs in low-back pain (LBP) among physiotherapists.

Methods
A cross-sectional study involving 45 consenting specialist musculoskeletal physiotherapists from three public-funded teaching hospitals in Nigeria was carried out. An adapted validated questionnaire on facilitators and barriers to CPRs utilization, and a socio-demographic proforma were used to collect data. Descriptive and inferential statistics were employed to analyze data. Alpha level was set at p < 0.05.

Results
Respondents were mostly males (71.1%), married (64.4%) and first-degree holders (55.6%). Twenty-eight (62.2%) of the respondents had above-average knowledge of CPRs in LBP. Rates for positive attitude towards, and utilization of CPRs were 37.8% and 15.6%. Knowledge and attitude about CPRs in LBP were not significantly influenced by socio-demographic factors (p > 0.05). However, there was a significant association between the utilization of CPRs and years of experience (χ2 = 10.339 p = 0.016).

Conclusion
Most Nigerian physiotherapists had above-average knowledge, but a negative attitude and low utilization of CPRs in LBP. Clinicians’ years of clinical experience influence the usage of CPR. There is a need to incorporate training in CPRs into undergraduate and continuous professional development programmes.
XAF1 antagonizes TRIM28 activity through the assembly of a ZNF313-mediated destruction complex to suppress tumor malignancy
Authors: Jang Seung-Hun , Choi Hwi-Wan , Ahn Jieun , Jang Sungchan , Yoon Ji-Hye , Lee Min-Goo , Chi Sung-Gil 
Published: November 2024

AbstractX-linked inhibitor of apoptosis-associated factor 1 (XAF1) is a stress-inducible pro-apoptotic protein that is commonly inactivated in multiple human cancers. Nevertheless, the molecular basis for its tumor suppression function remains largely uncharacterized. Here we report that XAF1 antagonizes the oncogenic activity of tripartite motif containing 28 (TRIM28) ubiquitin E3 ligase through zinc finger protein 313 (ZNF313)-induced ubiquitination and proteasomal degradation. XAF1 exerts apoptosis-promoting effect more strongly in TRIM28+/+ versus XAF1−/− tumor cells and suppresses tumor cell growth, migration, invasion, and epithelial-to-mesenchymal transition and xenograft tumor growth in a highly TRIM28-dependent fashion. Mechanistically, XAF1 interacts directly with the RING domains of TRIM28 and ZNF313 through the ZF6 and ZF7 domain, respectively, thereby facilitating ZNF313 interaction with and ubiquitination of TRIM28. A mutant XAF1 lacking either ZF6 or ZF7 domain exhibits no activity to promote TRIM28 ubiquitination. By destabilizing TRIM28, XAF1 blocks TRIM28-driven ubiquitination of p53 and RLIM, p53-HDAC1 interaction, and TWIST1 stabilization. Intriguingly, TRIM28 destabilizes XAF1 through K48-linked polyubiquitination and proteasomal degradation to protect tumor cells from apoptotic stress, indicating its role as an intrinsic antagonist against XAF1 and the antagonistic interplay of XAF1 and TRIM28. XAF1 expression is inversely correlated with TRIM28 expression in cancer cell lines and tumor tissues and more tightly associated with the survival of TRIM28-high versus TRIM28-low patients. Together, this study uncovers a novel mechanism by which XAF1 suppresses tumor malignancy and an important role for XAF1-TRIM28 interplay in governing stress response, illuminating the mechanistic consequence of its alteration during tumorigenic process.
Trunk climbing among ground-dwelling European amphibians – first observation from Poland
Authors: Kaczmarski Mikołaj , Kubicka Anna Maria 
Published: November 2024

Most European amphibian species are considered land-dwelling, associated with moving along the ground and as poor climbers. However, more recent research indicates that non-arboreal amphibians use nesting boxes and tree hollows in Central and Western Europe. This study describes the first observation of tree climbing by the common toad Bufo bufo (Linnaeus, 1758) and common frog Rana temporaria Linnaeus, 1758 in Poland. While conducting a night survey of newt mortality in the ‘Traszki Ratajskie’ protected area in an urban park in the city of Poznań, Poland, we detected the unexpected use of trees by two species of amphibians. In June 2020, we observed cases of Bufo bufo and Rana temporaria foraging on and using arboreal ambush sites. The toads were found at an average height of 124 cm above the ground, and the frogs at 35 cm, on the tree trunks and branches of two willows (Salix cfr. alba L.) and a dead silver birch specimen (Betula pendula L.). The willows had an extensive multi-stem structure, and exhibited signs of maturity and ageing (hollow-bearing trees with open cavities and crevices). This is the first observation of arboreal behaviour in B. bufo and R. temporaria in Poland. The climbing behaviour and use of tree sites were likely linked to the search for a humid shelter provided by the interior of the ageing trees, as well as to the foraging behaviour that we observed. Trees exhibiting signs of maturity usually have complex structures, and seem particularly important for amphibians during periods of drought, as both a suitable shelter and an area from which to ambush prey. We recommend focusing greater attention on the protection of mature, ageing trees, especially Salix spp. within a terrestrial buffer zone of at least 250 metres from ponds, as these are poorly researched components within amphibian habitats. This type of resource may be essential for long-term amphibian conservation due to site fidelity and the limited number of shelters in human-altered landscapes. The recently adopted EU Nature Restoration Law presents an opportunity to develop good practices in this area, particularly urban tree planting and habitat management.
Notes on the phally polymorphism in Vertigo lilljeborgi (Westerlund, 1871), V. geyeri Lindholm, 1925, V. genesii (Gredler, 1856) and V. ronnebyensis (Westerlund, 1871) in Sweden
Authors: von Proschwitz Ted , Książkiewicz Zofia 
Published: November 2024

In this paper we present some new information about the phally polymorphism of four Vertigo species: Vertigo lilljeborgi, V. geyeri, V. genesii and V. ronnebyensis. Material for examinations was collected from 7 sites in Sweden in 2021 and 2023. Despite a small number of individuals examined, all three sexual morphs (aphally, euphally, and hemiphally) were found in V. geyeri and V. genesii. Hemiphallics were recorded in these two species for the first time. In V. lilljeborgi we only found aphallics and euphallics, while in V. ronnebyensis only aphallics were found.
La geografía y la guerra, los geógrafos y la paz: ampliando las agendas políticas y de investigación
Authors: Mamadouh Virginie 
Published: November 2024

La relación estrecha entre la disciplina de la geografía y la violencia militar en proyectos tanto nacionalistas como imperialistas está bien establecida. Sin embargo, hasta hace poco, la guerra rara vez ha sido un tema prominente en las investigaciones geográficas. Aún menos se ha tratado la paz como objeto de estudio, a pesar de que siempre ha habido geógrafos que han intentado usar la geografía para promover la paz o deslegitimar la guerra y la violencia. Este trabajo ofrece una visión de las numerosas agendas políticas y de investigación que se han desarrollado recientemente en la geografía en relación con la guerra y la paz
Navigating the digital frontier: transforming endodontic diagnosis through digitization. part 1
Authors: D'Souza Janina Loren , Mala Kundabala , Grover Srishti , Singh Ankita 
Published: November 2024

The current advances and innovations in material technology and armamentaria help to negate the difficulties faced by clinicians in diagnosing and treating patients. Oral healthcare contributes much to the wellbeing of a person. However, the oral cavity is a small and difficult area in which to work since it is filled with vascularized mobile tissues and various shaped teeth, and connected to sinuses, nerve canals, lungs and the gastrointestinal tract via the trachea and pharynx. Moreover, each tooth has a complex root canal system that must be explored if it is infected or inflamed. Root canal treatment can be compared to entering a blind tunnel and treating something unseen. Endodontics is a field in dentistry where a clinician must use their tactile sense and correlate it with clinical knowledge, as well as skill, to treat dental infections. Digital evolution has simplified diagnosis, treatment planning, documentation and communication with patients, rendering more predictable outcomes and providing evidence for the healing or non-healing of disease. The evolution of digital radiography has offered us the best technology available. The present review discusses various helpful digital tools that are currently available, and future technologies that may guide clinicians in better diagnosis. CPD/Clinical Relevance: Advances in diagnostic tools have improved accuracy in identifying and treating dental pathologies, thereby achieving more predictable outcomes and enhancing patient care.
Brachioradialis tendon transfer and palmaris longus tendon graft for thumb avulsion: A case report and review of literature
Authors: Curings Pierre , Ramos-Pascual Sonia , Michalewska Kinga , Gibert Nicolas , Erhard Lionel , Saffarini Mo , Nogier Alexis 
Published: November 2024

BACKGROUND
Thumb replantation following complete traumatic avulsion requires complex techniques to restore function, especially in cases of avulsion at the level of the metacarpophalangeal joint (MCP I) and avulsion of the flexor pollicis longus (FPL) at the musculotendinous junction. Possible treatments include direct tendon suture or tendon transfer, most commonly from the ring finger. To optimize function and avoid donor finger complications, we performed thumb replantation with flexion restoration using brachioradialis (BR) tendon transfer with palmaris longus (PL) tendon graft.
CASE SUMMARY
A 20-year-old left-handed male was admitted for a complete traumatic left thumb amputation following an accident while sliding from the top of a handrail. The patient presented with skin and bone avulsion at the MCP I, avulsion of the FPL tendon at the musculotendinous junction (zone 5), avulsion of the extensor pollicis longus tendon (zone T3), and avulsion of the thumb’s collateral arteries and nerves. The patient was treated with two stage thumb repair. The first intervention consisted of thumb replantation with MCP I arthrodesis, resection of avulsed FPL tendon and implantation of a silicone tendon prosthesis. The second intervention consisted of PL tendon graft and BR tendon transfer. Follow-up at 10 months showed good outcomes with active interphalangeal flexion of 70°, grip strength of 45 kg, key pinch strength of 15 kg and two-point discrimination threshold of 4 mm.
CONCLUSION
Flexion restoration after complete thumb amputation with FPL avulsion at the musculotendinous junction can be achieved using BR tendon transfer with PL tendon graft.
Bullous pemphigoid associated with acquired hemophilia A: A case report
Authors: Hu Su-Ye , Li Meng-Can , Hao Zi-Jia , Chai Xu-Ya , Li Pei-Sai , Liu Yang , Liu Li-Xia , Xu Ying , Yang Pan-Pan , Li Ling-E 
Published: November 2024

BACKGROUND
Acquired hemophilia A (AHA) is a rare and potentially severe bleeding disorder caused by circulating autoantibodies against factor VIII (FVIII). In approximately 50% of the patients, the condition is associated with autoimmune diseases, cancers, medication use, pregnancy, and the post-partum period. Bullous pemphigoid (BP) is a chronic autoimmune subepidermal blistering disease associated with tissue-bound and circulating autoantibodies against BP antigens 180 (BP180) and 230 (BP230). AHA-associated BP has a high mortality rate; hence, the understanding of this disease must improve.
CASE SUMMARY
A 69-year-old man presented with erythema, blisters, blood blisters, and crusts accompanied by severe pruritus for more than 20 days, and ecchymosis and swelling on his left upper arm for 3 days. Pathological examination revealed a subepidermal blister that contained eosinophils. Laboratory tests showed that the BP180 autoantibody levels had increased, isolated activated partial thromboplastin time was notably prolonged (115.6 s), and coagulation FVIII activity was extremely low (< 1.0%). Furthermore, the FVIII inhibitor titer had greatly increased (59.2 Bethesda units). Therefore, the patient was diagnosed as having BP associated with AHA, prescribed 0.05% topical halometasone cream, and transferred to a higher-level hospital for effective treatment; however, he died after 2 days.
CONCLUSION
AHA associated BP is rare, dangerous, and has a high mortality rate. Therefore, its timely diagnosis and effective treatment are necessary.
Analysis of Earthquake Vulnerability of The Demak Coastal Area based on the HVSR (Horizontal To Vertical Spectral Ratio) Method
Authors: Nurwidyanto Muhammad Irham , Harmoko Udi , Gernowo Rahmat , Fernando Gregrius Alvin , Koesuma Sorja 
Published: November 2024

<p>Most of the Demak Coastal area, especially on the north coast, has a subsurface structure which is quite thick sediment. The impact of an earthquake will cause damage to subsurface structures and buildings above it, so a method is needed that can determine earthquake vulnerability in an area to take mitigation steps. This research aims to analyze vulnerability to earthquakes based on natural frequency (fo), amplification (Ao) and seismic vulnerability index (SVI). The research was carried out by measuring the microseismic signal response at 89 locations using a 3-component seismograph and data logger. The research results showed that the dominant frequency varied from 0.26 – 5.26 Hz, the amplification factor varied from 0.51 – 3.56 and the seismic susceptibility index varied from 0.14-14.77 micro cm<sup>2</sup>/s. The study area was classified into low SVI (SVI &lt; 5), medium SVI (5 &lt; SVI &lt; 10), and high SVI (SVI &gt; 10). The potential earthquake hazard described by SVI with a value range of 10 &lt; SVI (high classification) is found at observation station 60 (Bedono Hamlet) and observation station 70 (Karangwaru Hamlet Cemetery)</p>
Association between coordinated counseling from both ASHA and Anganwadi Workers and maternal health outcomes: A cross-sectional study from Madhya Pradesh and Bihar, India
Authors: Gopalakrishnan Lakshmi , Patil Sumeet , Fernald Lia , Walker Dilys , Diamond-Smith Nadia 
Published: November 2024

Community Health Workers (CHWs) play crucial roles in health promotion and services in rural India. Previous research investigating the effectiveness of coordinated health promotion by different community health workers (CHWs) cadres on health practices is scarce. This study examines the effectiveness of coordinated health promotion by different CHW cadres, specifically Accredited Social Health Activists (ASHA) and Anganwadi Workers (AWW), on maternal health outcomes. Using endline data from a 2019 impact evaluation of 6635 mothers with children <12 months in Madhya Pradesh and Bihar, we compared the association between standalone and coordinated counseling by ASHA and AWW on various maternal health practices. Outcomes included four or more antenatal care visits, birth preparedness, institutional delivery, postnatal care visits, and contraceptive uptake. Fixed effects logistic regression with robust standard errors, corrected for multiple hypothesis tests, was used for analysis. Results showed that 39.6% of women received four or more ANC visits, 31.2% adopted birth preparedness practices, 79.6% had institutional deliveries, 23.3% received postnatal care, and 19.5% adopted a postpartum contraceptive method. Coordinated counseling from both ASHA and AWW was associated with a greater prevalence of four outcomes (birth preparedness, institutional delivery, PNC visit, and contraception) compared to standalone counseling from either ASHA or AWWs. These findings suggest that health promotion by AWW complements that of ASHA, collectively associated with improved health outcomes. This study underscores the effectiveness of coordinated health promotion and highlights the need for multisectoral and coordinated efforts among different CHW cadres at the community level. The results emphasize the importance of integrating various CHW roles to enhance maternal health practices and outcomes in rural India.
Trial registration number: https://doi.org/10.1186/ISRCTN83902145.
Date of registration: 08/12/2016.