bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential use of leukocytosis and anion gap elevation in differentiating psychogenic nonepileptic seizures from epileptic seizures
Authors: Li Yi , Matzka Liesl , Flahive Julie , Weber Daniel 
Published: January 2019

SummaryEpileptic seizures (ES) and psychogenic nonepileptic seizures (PNES) can be difficult to differentiate from each other in the emergency department (ED) setting. We have previously shown that the anion gap (AG) can help differentiate between ES and PNES in the ED. In this study, we explored whether additionally considering leukocytosis can help better differentiate between ES and PNES. We screened a total of 1354 subjects seen in the ED of a tertiary care medical center; 27 PNES and 27 ES patients were identified based on clinical description and subsequent electroencephalography (EEG). Multivariable logistic regression analysis was used to model the association between ES, leukocytosis, and AG. Our results indicated that within 9 hours after the index event, serum AG (adjusted odds ratio [aOR] 2.07) and white blood cell (WBC) count (aOR 1.61) were both independently associated with ES. We derived an equation to help differentiate between ES and PNES: 1.5*AG+WBC. A score >24.8 indicated a >90% likelihood of ES. A score <15.5 indicated a <10% likelihood of ES (ie, the alternate diagnosis of PNES should be considered). This study for the first time provides evidence to help differentiate PNES and ES utilizing acidosis and leukocytosis.
Efficacy of psychotherapy for bulimia nervosa and binge‐eating disorder on self‐esteem improvement: Meta‐analysis
Authors: Linardon Jake , Kothe Emily J. , Fuller‐Tyszkiewicz Matthew 
Published: January 2019

AbstractObjectivesThis meta‐analysis examined the effects of psychotherapy for bulimia nervosa (BN) and binge‐eating disorder (BED) on self‐esteem improvement.MethodRandomized controlled trials (RCTs) of psychological treatments that assessed self‐esteem change in eating disorders were included. Thirty‐four RCTs were included; most sampled BED and then BN. Hedge's g effects were entered into random effects models.ResultsPsychotherapy for BN led to significantly greater post‐treatment improvements in self‐esteem than control conditions (g = 0.45; 95% CI [0.17, 0.73]). This effect was smaller when only analysing low risk of bias trials (g = 0.28; 95% CI [0.05, 0.51]). Psychotherapy for BED also led to significantly greater post‐treatment improvements in self‐esteem than controls (g = 0.20; 95% CI [0.05, 0.35]), with some evidence that guided self‐help was associated with the largest effects. This effect, however, was overestimated after adjustment for publication bias (g = 0.10; 95% CI [−0.05, 0.26]). There was no evidence that cognitive‐behavioural therapy was superior to non‐cognitive‐behavioural therapy interventions in improving self‐esteem. There was no relationship between symptom improvement and self‐esteem improvement in a meta‐regression.ConclusionsPsychotherapy may lead to small improvements in self‐esteem in BN and BED. Additional RCTs with follow‐up assessments are required to make more definitive conclusions about the effects of psychotherapy for eating disorders on self‐esteem in the long‐term.
Netzwerk des Überlebens
Authors: Rössler Patrick , Droste Magdalena , Blümm Anke 
Published: January 2019

AbstractVor 100 Jahren gegründet, gilt das Bauhaus als der erfolgreichste deutsche Kulturexport des 20. Jahrhunderts. Eine Studie verdeutlicht, wie der kommunikative Austausch zwischen den Bauhaus‐Angehörigen die Institution auch nach ihrer Schließung 1933 als eine frühe Form „virtueller Gemeinschaft” fortleben ließ. Ein neuer Blick jenseits üblicher, von stilistischen Überlegungen geprägter Betrachtungen.
Flexible model for patient engagement: Achieving quality outcomes and building a research agenda for head and neck cancer
Authors: Iwata Ayaka J. , Olden Heather A. , Kippen Karen E. , Swegal Warren C. , Johnson Christine C. , Chang Steven S. 
Published: January 2019

AbstractBackgroundThis article describes the benefits of patient‐driven research in the field of head and neck oncology, reviews lessons learned from establishing partnerships with patients and caregivers, and serves as a model for further patient‐driven research endeavors.MethodsHead and neck cancer survivors underwent training including that of effective communication and the basics of research methodology. They then drove the agendas for monthly meetings that included a multidisciplinary team of providers, facilitated by a physician champion (S.S.C.).ResultsThe advisors reported concrete areas for improvement of the clinical flow, including the formation of a dental oncology clinic and a post‐treatment survivorship clinic. They also refined research topics of interest, such as treatment regret. The advisors have also driven efforts to increase public awareness and have participated in cancer symposiums and local presentations.ConclusionPatient‐driven research improves the relevance and implementation of head and neck oncology research and clinical processes.
Trade‐off Analytics: Creating and Evaluating the Tradespace
Authors: Parnell Gregory S. 
Published: January 2019

ABSTRACTThe INCOSE Corporate Advisory Board documented the need for a guidebook on best practices for trade studies. They wanted a book that covered the system lifecycle and aligned with INCOSE process guidance (ISO/IEC 15288, Systems Engineering Handbook, and the “Systems Engineering Body of Knowledge”). The INCOSE Decision Analysis Working Group supported the book project. Members of the Decision Analysis Working Group began by writing the decision management sections of the Systems Engineering Handbook and the “Systems Engineering Body of Knowledge” (SEBoK). We decided to develop a textbook in the Wiley Systems Engineering and Management Series to meet this need (note: the authors provide all proceeds from sale of the textbook directly to the INCOSE Foundation). Our goal was to describe and illustrate sound trade‐off analysis techniques to identify value and risk to help decision makers make the necessary trade‐offs to provide affordable systems that meet performance and schedule objectives. We recruited sixteen leading systems engineering experts with significant academic and industry experience. The textbook audience is undergraduate students (systems engineering, industrial, engineering management, other engineering); graduate students (systems, industrial, engineering management, other engineering); and professional systems engineers, project managers, and engineering managers. The textbook can also be used as a professional reference. Wiley offers a 25% discount to INCOSE Corporate Advisory Board members.
Wildfire Detection and Communication–Aerospace Applications–Trade Study
Authors: Crawford Setrige W. , Shahroudi Kamran Eftekhari 
Published: January 2019

ABSTRACTWildfires have increased in frequency, duration, and intensity worldwide. Climate change, drought, and other factors have not only increased susceptibility to wildfires, but have also increased the duration of the season. There are a number of factors affecting wildfires: detection, speed of communication/response time, resources/politics/climate change/infrastructure to fight fires, and prevention education. A wildfire doubles in size and intensity every 3 to 5 minutes and response times tend to be 10 to 15 minutes at best. The goal of the trade analysis is to arrive at a cost‐effective and robust performance system which can be operated at the county level with minimal infrastructure to mitigate the menacing problem of forest wildfires. The approach will be a disciplined systems engineering approach to objectively arrive at the best solution for detection and communication of wildfires, having analyzed the measures of effectiveness (MOEs) for all critical requirements for a technologically diverse set of solutions. Though the analysis is still at a very early stage and the outcome could change as additional details are developed, due diligence was exercised in the evaluation of parameters such as detection time, total operations cost, and operational flexibility, to name a few. Early trade‐off results indicate that the lead concept is a rotor‐wing unmanned aerial vehicle (UAV) concept, utilizing a rotorcraft configuration which could be outfitted with a remote‐sensing payload compliment based on light detection and ranging (LiDAR) technology with associated functional equipment such as global positioning systems (GPS) and inertial measurement units (IMU). The UAV would be semiautonomous, launched from and remotely controlled by an operator in the general area of interest. Upon arriving at this area of interest, the UAV would then fly a flight plan autonomously to collect and communicate data to a base station to be used to direct wildfire mitigation services in the event of a positive detection.
Zinc oxide nanoparticles induced gene mutation at the HGPRT locus and cell cycle arrest associated with apoptosis in V‐79 cells
Authors: Jain Abhishek Kumar , Singh Divya , Dubey Kavita , Maurya Renuka , Pandey Alok Kumar 
Published: January 2019

AbstractIn recent years, the large‐scale production of ZnO nanoparticles (NPs) for various applications is increasing exponentially and may pose serious health issues when inhaled either during occupational exposure or in consumer settings. The mechanisms underlying the toxicity of NPs have recently been studied intensively. Despite the existing studies, the mutagenicity of ZnO NPs in the eukaryotic system is still unclear. Therefore, the aim of the present study was to investigate the mutagenic potential of ZnO NPs using Chinese hamster lung fibroblast cells (V‐79) as an in‐vitro model. The study has demonstrated a significant uptake of ZnO NPs by flow cytometry with the confirmation of transmission electron microscopy. A reduction in cell viability was observed with a concomitant increase in reactive oxygen species (**P < 0.01, ***P < 0.001) after ZnO NP (1‐20 μg/mL) exposure. Excessive reactive oxygen species can induce oxidative stress, which leads to genotoxic insult, and further gene mutation. Apart from measuring the genotoxicity by Comet assay, a change of 2.84‐fold in the HGPRT gene mutant frequency was observed by the mammalian gene forward mutation assay. All the genotoxicity endpoints such as chromosomal break, DNA damage and mutagenicity were observed at 6 hours of ZnO NP exposure. Our results also showed that ZnO NPs manifested the cell cycle arrest, ultrastructural modifications and further cell death. A significant (**P < 0.01, ***P < 0.001) increase in the apoptotic cells was detected using annexin V‐fluorescein isothiocyanate/propidium iodide double staining by flow cytometry. Our findings presented here clearly stimulate the need for careful regulations of ZnO NPs.
The Novel p38 Inhibitor, Pamapimod, Inhibits Osteoclastogenesis and Counteracts Estrogen-Dependent Bone Loss in Mice
Authors: Zhao Xiangde , Ning Lei , Xie Ziang , Jie Zhiwei , Li Xiang , Wan Xinyu , Sun Xuewu , Huang Bao , Tang Pan , Shen Shuying , Qin An , Ma Yan , Song Lu , Fan Shunwu , Wan Shuanglin 
Published: January 2019

ABSTRACT

Pamapimod (PAM) is a novel selective p38 mitogen-activated protein (MAP) kinase inhibitor proved to be effective in rheumatoid arthritis in phase 2 clinical trial. However, its effect on osteoclast-associated osteoporosis and the underlying mechanisms remain unclear. In this study, we showed that PAM suppressed receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation via inhibition of p38 phosphorylation and subsequent c-Fos and nuclear factor of activated T cells c1 (NFATc1) expression. In addition, the downregulated NFATc1 leads to reduced expression of its targeting gene disintegrin and metalloproteinase domain-containing protein 12 (ADAM12), which was further proven to be critical for osteoclastic bone resorption. Therefore, we treated ovariectomized (OVX) mice with PAM and revealed a protective effect of PAM on osteoporosis in vivo. In conclusion, our results demonstrated PAM can prevent OVX-induced bone loss through suppression of p38/NFATc1-induced osteoclast formation and NFATc1/ADAM12-associated bone resorption. © 2018 American Society for Bone and Mineral Research.
Body mass index and diabetes are important prognostic signatures for bilateral breast cancer prognosis
Authors: Zhang Min , Zhang Xiaobei , Liu Jingjing , Su Wei , Li Jiao , Zhang Sheng , Zhang Jin 
Published: January 2019

AbstractObjectiveSeveral research have suggested that high body mass index (BMI) and diabetes were related to poor breast cancer prognosis. The purpose of this study was to investigate the relationships between BMI/diabetes and the prognosis of bilateral breast cancer (BBC).MethodsA total of 26 992 patients with primary breast cancer underwent operations at the Tianjin Medical University Cancer Institute and Hospital between January 1971 and November 2011. Among them, 512 consecutive patients with BBC and 1024 patients with unilateral breast cancer (UBC) were retrospectively reviewed. Overall survival (OS) and progression‐free survival (PFS) rates after tumor removal were estimated with Kaplan‐Meier method followed by log‐rank test for evaluating their differences that stratified by BMI (<25, 25~30, ≥30) or diabetes (suffer/not suffer). Multivariate Cox proportional hazards regression model was used to uncover the relationship between common clinical pathological features and prognosis of breast cancers.ResultsCompared with UBC, patients with BBC tended to be with higher BMI (P = 0.000) and larger percentage of diabetes (
P = 0.035). The Kaplan‐Meier survival analysis indicated that higher BMI complicated by diabetes and negative estrogen receptor significantly decreased the survival rate of BBC patient (
P < 0.05). In addition, diabetes was closely associated with OS or PFS in metachronous BBC (
P = 0.006). The result of multivariate Cox proportional hazards regression model showed that BMI and diabetes were two independent prognostic factors for OS.ConclusionObesity and diabetes have a significant impact on the survival of the patients with BBC.
Altered frequency of CD8+CD11c+ T cells and expression of immunosuppressive molecules in lymphoid organs of mouse model of colorectal cancer
Authors: Rostamzadeh Davood , Haghshenas Mohammad Reza , Daryanoosh Farhad , Samadi Mahdi , Hosseini Ahmad , Ghaderi Abbas , Mojtahedi Zahra , Babaloo Zohreh 
Published: January 2019

AbstractCD11c is a member of the β2‐integrin family typically used to define myeloid dendritic cells (DCs). Recent reports identify CD11c‐expressing CD8+ T cells as a new subset of CD8+ regulatory T cells (Treg). Evidence exists that CD11c+CD8+ T cells may exert their effector or regulatory functions under different conditions. To date, no studies have addressed the frequency of CD11c+ T cells in cancer. Limited evidence exists in terms of expression of immune‐checkpoint receptors, programmed cell death protein 1 (PD‐1) and T‐lymphocyte‐associated antigen 4 (CTLA‐4), as well as forkhead box P3 (Foxp3) in mouse lymphoid organs. Here, we have assessed CD11c+CD8+ and CD11c+CD4+ T cells, Foxp3, PD‐1, and CTLA‐4 expressing CD4+ T cells and CD8+ T cells in different tissues from three groups of male BALB/c mice—young, mature, and those with colorectal cancer (CRC). Analysis of CD3+CD11c+ T cells in the bone marrow (BM), spleen, and lymph nodes (LN) in each group showed a higher percentage of CD3+CD11c+ T cells in the BM from all groups and in the lymphoid organs of the cancer group compared with the young and mature groups. CD4low and CD4high cell fractions in mice BM have different expression patterns for Foxp3 and CTLA‐4. We have observed a higher frequency of CD8+PD‐1+ T cells in the BM, spleen, and LN of CRC mice compared with normal mice. T‐cell exhaustion is associated with inhibitory receptor PD‐1. According to the regulatory roles of CD11c expression in CD8+ T cells, we have proposed that the elevated percentage of CD11c, Foxp3, CTLA‐4, and PD‐1 expressing T cells were associated with immune response dysregulation in CRC.
Role of SNHG7‐miR‐653‐5p‐STAT2 feedback loop in regulating neuroblastoma progression
Authors: Chi Renjie , Chen Xin , Liu Ming , Zhang Huanyu , Li Fujiang , Fan Xu , Wang Weiwei , Lu Hongting 
Published: January 2019

AbstractLong noncoding RNAs (lncRNAs) are reported to be involved in the pathology of numerous cancers, including neuroblastoma (NB). lncRNA SNHG7 has been recognized as a carcinogen in several cancers, but its role in NB progression remains unknown. Our study revealed that SNHG7 expression was markedly higher in NB tissues than that in nontumor tissues. Besides, upregulated SNHG7 was greatly correlated with poor overall survival of NB patients. Functionally, the loss‐of‐function assays demonstrated that knockdown of SNHG7 inhibited cell proliferation, migration, invasion, and epithelial–mesenchymal transition in NB cells. Mechanically, the bioinformatics analysis predicted that miR‐653‐5p was the shared partner of SNHG7 and signal transducer and activator of transcription 2 (STAT2). Unsurprisingly, we further confirmed that SNHG7 could interact with miR‐653‐5p and therefore functioned as the ceRNA of STAT2 so as to regulate STAT2 expression in NB cells. Moreover, STAT2 expression was in inverse proportion to miR‐653‐5p level but in positive proportion to SNHG7 level in NB tissues. Importantly, the repressed NB progression induced by silenced SNHG7 was reversed by STAT2 overexpression or miR‐653‐5p inhibitors. Jointly, our findings elucidated SNHG7 facilitated NB progression through the miR‐653‐5p/STAT2 pathway, providing a novel therapeutic target and prognostic biomarker for this disease.
Pooling analysis reveals that galectin‐1 is a reliable prognostic biomarker in various cancers
Authors: Huang Ming‐Yu , He Jia‐Peng , Zhang Wen‐Qian , Liu Ji‐Long 
Published: January 2019

AbstractGalectin‐1 is reported to be upregulated in various human cancers. However, the relationship between galectin‐1 expression and cancer prognosis has not been systematically assessed. In this study, we searched PubMed, Web of Science, and Embase to collect all relevant studies and a meta‐analysis was performed. We found that increased galectin‐1 expression was associated with tumor size (odds ratio [OR] = 1.75; 95% confidence interval [CI]: 1.06–2.89; p = 0.029), clinical stage (OR = 3.89; 95% CI: 2.40–6.31; p < 0.001), and poorer differentiation (OR = 1.39; 95% CI: 1.14–1.69; p = 0.001), but not with age (OR = 1.07; 95% CI: 0.82–1.39; p = 0.597), sex (OR = 0.89; 95% CI: 0.74–1.07; p = 0.202), or lymph node metastasis (OR = 2.57; 95% CI: 0.98–6.78; p = 0.056). In addition, we found that high galectin‐1 expression levels were associated with poor overall survival (HR = 2.12; 95% CI: 1.71–2.64; p < 0.001). The results were further validated using The Cancer Genome Atlas data set. Moreover, high galectin‐1 expression was significantly associated with disease‐free survival (hazard ratio [HR] = 1.60; 95% CI: 1.17–2.19; p = 0.003), progression‐free survival (HR = 1.93; 95% CI: 1.65–2.25; p < 0.001), and cancer‐specific survival (HR = 1.82; 95% CI: 1.30–2.55; p < 0.001). Our meta‐analysis demonstrated that galectin‐1 might be a useful common biomarker for predicting prognosis in patients with cancer.
Long noncoding RNA MNX1‐AS1 contributes to lung cancer progression through the miR‐527/BRF2 pathway
Authors: Liu Haibo , Han Leng , Liu Zhengjia , Gao Nan 
Published: January 2019

AbstractLung cancer belongs to a leading popular and malignant cancer around the world. However, the root mechanism underlying lung cancer progression remains unclear. Recently, long noncoding RNA (lncRNA) has been identified as important for tumorigenesis. LncRNA MNX1‐AS1 is proven to regulate colon adenocarcinoma, cervical cancer, glioblastoma, and ovarian cancer. Whether MNX1‐AS1 participates in lung cancer needs investigation. In our research, we found that MNX1‐AS1 was dramatically upregulated in lung cancer. MNX1‐AS1 upregulation indicated poor prognosis in lung cancer patients. Functionally, MNX1‐AS1 promoted lung cancer progression through regulating proliferation, migration, and invasion. Mechanistically, MNX1‐AS1 was found to locate in the cytoplasm and interact with miR‐527. Through inhibiting miR‐527 availability, MNX1‐AS1 facilitated BRF2 expression. Restoration of BRF2 rescued defects of proliferation, migration, and invasion caused by MNX1‐AS1 knockdown. Taken together, our study found a novel signaling pathway, namely MNX1‐AS1/miR‐527/BRF2 axis, involved in lung cancer progression.
Receptor activity‐modifying protein 1 regulates the phenotypic expression of BMSCs via the Hippo/Yap pathway
Authors: Zhang Qin , Guo Yanjun , Yu Hui , Tang Yufei , Yuan Ying , Jiang Yixuan , Chen Huilu , Gong Ping , Xiang Lin 
Published: January 2019

AbstractReceptor activity‐modifying protein 1 (RAMP1) might be a critical regulator during bone wound healing. However, the roles and mechanisms of RAMP1 in osteogenesis remain unclear. Here, we aimed to elucidate the role of RAMP1 and explore the effects of Yes‐associated protein 1 (Yap1), an effector of the Hippo/Yap pathway, in this process. We used a RAMP1 overexpression lentiviral system in bone marrow mesenchymal stem cells (BMSCs), which enhanced RAMP1 expression in an effective, appropriate, and sustained manner. Alkaline phosphatase (ALP) activity assays and alizarin red staining showed that RAMP1 promoted osteogenic differentiation of BMSCs after calcitonin gene‐related peptide (CGRP) treatment (10
−8 mol/L). Moreover, real‐time polymerase chain reaction and Western blot analysis indicated that RAMP1 upregulated the expression of osteogenic phenotypic markers (ALP, runt‐related transcription factor 2, osteopontin;
p < 0.05). To further uncover the mechanism of RAMP1 in osteogenic differentiation, we used verteporfin (10
−7 mol/L) to block Yap1. Notably, verteporfin impaired RAMP1‐induced osteogenesis. Taken together, our findings confirmed that RAMP1 is a key mediator of bone regeneration and indicate that RAMP1 promotes CGRP‐induced osteogenic differentiation of BMSCs via regulation of the Hippo/Yap pathway.