bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cerebrospinal Fluid Concentrations of Nimodipine Correlate With Long-term Outcome in Aneurysmal Subarachnoid Hemorrhage: Pilot Study
Authors: Riva Roberto , Pegoli Marianna , Contin Manuela , Perrone Alessandro , Mohamed Susan , Zanello Marco 
Published: July 2019

Objectives
The aim was to evaluate plasma and cerebrospinal fluid (CSF) nimodipine concentrations in patients with aneurysmal subarachnoid hemorrhage and their correlation with clinical outcome.

Methods
Nimodipine infusion was started at 1 mg/h and increased up to 2 mg/h and continued up to 21 days in surviving patients. Arterial and CSF samples were collected at least after 24 hours of stable nimodipine dosing. Delayed cerebral ischemia and vasospasm were documented by new neurological deficits and neuroimaging. The clinical outcome was assessed at 9 months by the modified Rankin scale.

Results
Twenty-three patients were enrolled. Nimodipine dose was 13 to 38 μg/kg per hour. Nimodipine arterial and CSF concentrations were 24.9 to 71.8 ng/mL and 37 to 530 pg/mL, respectively. Dose did not correlate with arterial or CSF concentrations. Arterial concentrations did not correlate with corresponding CSF concentrations. Doses and arterial concentrations did not correlate with the clinical outcome and were not associated with the occurrence of delayed cerebral ischemia. However, patients with no significant disability after 9 months of hemorrhage showed significantly higher CSF nimodipine concentrations (P = 0.015) and CSF-to-plasma ratios (P = 0.011) compared with patients who showed some degree of disability or who died.

Conclusions
Cerebrospinal fluid nimodipine concentrations measured during hospital drug infusion showed a correlation with long-term clinical outcome in patients with aneurysmal subarachnoid hemorrhage. These very preliminary data suggest that CSF concentrations monitoring may have some value in managing these patients.
Kinship ties across the lifespan in human communities
Authors: Koster Jeremy , Lukas Dieter , Nolin David , Power Eleanor , Alvergne Alexandra , Mace Ruth , Ross Cody T. , Kramer Karen , Greaves Russell , Caudell Mark , MacFarlan Shane , Schniter Eric , Quinlan Robert , Mattison Siobhan , Reynolds Adam , Yi-Sum Chun , Massengill Eric 
Published: July 2019

A hypothesis for the evolution of long post-reproductive lifespans in the human lineage involves asymmetries in relatedness between young immigrant females and the older females in their new groups. In these circumstances, inter-generational reproductive conflicts between younger and older females are predicted to resolve in favour of the younger females, who realize fewer inclusive fitness benefits from ceding reproduction to others. This conceptual model anticipates that immigrants to a community initially have few kin ties to others in the group, gradually showing greater relatedness to group members as they have descendants who remain with them in the group. We examine this prediction in a cross-cultural sample of communities, which vary in their sex-biased dispersal patterns and other aspects of social organization. Drawing on genealogical and demographic data, the analysis provides general but not comprehensive support for the prediction that average relatedness of immigrants to other group members increases as they age. In rare cases, natal members of the community also exhibit age-related increases in relatedness. We also find large variation in the proportion of female group members who are immigrants, beyond simple traditional considerations of patrilocality or matrilocality, which raises questions about the circumstances under which this hypothesis of female competition are met. We consider possible explanations for these heterogenous results, and we address methodological considerations that merit increased attention for research on kinship and reproductive conflict in human societies.This article is part of the theme issue ‘The evolution of female-biased kinship in humans and other mammals’.
One piece of the matrilineal puzzle: the socioecology of maternal uncle investment
Authors: Starkweather Kathrine , Keith Monica 
Published: July 2019

Maternal uncle relationships in which men invest resources (usually in the form of inheritance of material wealth) into their sisters' children are characteristic of matrilineal systems and hypothesized to arise under certain socioecological circumstances, but little research has systematically investigated conditions that are associated with this type of investment. We quantify relationships between household-level socioeconomic variables and different types of maternal uncle investment (direct care and indirect resource investment) within a bilateral, semi-nomadic population. Shodagor people of Bangladesh allow us to consider matrilineal behaviours in an evolutionary framework owing to their flexible social structure in which 39% of families receive some investment from a maternal uncle. Variables associated with direct maternal uncle care reflect the significance of maintaining consistent residence throughout the year and an increased need for childcare in families residing on boats versus those living on the land. Informative predictors of indirect investment indicate that a mother's birth history corresponds with more tangible contributions such as food and clothing. These results identify household-level variables specific to direct versus indirect maternal uncle investment, whereas having more older brothers or being firstborn increased the odds of a mother receiving any investment from brothers at all. Exploring these social and ecological associations in a bilateral, relatively flexible population unveils household circumstances that may lead to the development of female-biased kinship.
This article is part of the theme issue ‘The evolution of female-biased kinship in humans and other mammals'.
How can non-human primates inform evolutionary perspectives on female-biased kinship in humans?
Authors: Emery Thompson Melissa 
Published: July 2019

The rarity of female-biased kinship organization in human societies raises questions about ancestral hominin family structures. Such questions require grounding in the form and function of kin relationships in our close phylogenetic relatives, the non-human primates. Common features of primate societies, such as low paternity certainty and lack of material wealth, are consistent with features that promote matriliny in humans. In this review, I examine the role of kinship in three primate study systems (socially monogamous species, female-bonded cercopithecines and great apes) that, each for different reasons, offer insights into the evolutionary roots of matriliny. Using these and other examples, I address potential analogues to features of female-biased kinship organization, including residence, descent and inheritance. Social relationships are biased towards matrilineal kin across primates, even where female dispersal limits access to them. In contrast to the strongly intergenerational nature of human kinship, most primate kin relationships function laterally as the basis for cooperative networks and require active reinforcement. There is little evidence that matrilineal kin relationships in primates are functionally equivalent to descent or true inheritance, but further research is needed to understand whether human cultural constructs of kinship produce fundamentally different biological outcomes from their antecedents in primates.
This article is part of the theme issue ‘The evolution of female-biased kinship in humans and other mammals'.
When does matriliny fail? The frequencies and causes of transitions to and from matriliny estimated from a de novo coding of a cross-cultural sample
Authors: Shenk Mary K. , Begley Ryan O. , Nolin David A. , Swiatek Andrew 
Published: July 2019

The question of when and why societies have transitioned away from matriliny to other types of kinship systems—and when and why they transition towards matriliny—has a long history in anthropology, one that is heavily engaged with both evolutionary theory and cross-cultural research methods. This article presents tabulations from a new coding of ethnographic documents from the Standard Cross-Cultural Sample (SCCS), tallying claims of transitions in kinship systems both away from and to matriliny using various levels of stringency. We then use our counts as the outcome variables in a set of Bayesian analyses that simultaneously estimate the probability of a transition occurring given societal covariates alongside the conditional probability of detecting a transition given the volume of ethnographic data available to code. Our goal is to estimate the cross-cultural and comparative frequency of transitions away from and to matriliny, as well as to explore potential causes underlying these patterns. We find that transitions away from matriliny have been significantly more common than ‘reverse transitions' to matriliny. Our evidence suggests that both rates may be, in part, an artefact of the colonial and globalizing period during which the data comprising much of the current ethnographic record were recorded. Analyses of the correlates of transitions away from matriliny are consistent with several of the key causal arguments made by anthropologists over the past century, especially with respect to subsistence transition (to pastoralism, intensive agriculture and market economies), social complexity and colonialism, highlighting the importance of ecological factors in such transitions.This article is part of the theme issue ‘The evolution of female-biased kinship in humans and other mammals’.
Confounding adjustment performance of ordinal analysis methods in stroke studies
Authors: Zonneveld T.P. , Aigner A. , Groenwold R.H.H. , Algra A. , Nederkoorn P.J. , Grittner U. , Kruyt N.D. , Siegerink B. 
Published: July 2019

AbstractBackgroundIn acute stroke studies, ordinal logistic regression (OLR) is often used to analyze outcome on the modified Rankin Scale (mRS), whereas the non-parametric Mann-Whitney measure of superiority (MWS) has also been suggested. It is unclear how these perform comparatively when confounding adjustment is warranted. Our aim is to quantify the performance of OLR and MWS in different confounding variable settings.MethodsWe set up a simulation study with three different scenarios; (1) dichotomous confounding variables, (2) continuous confounding variables, and (3) confounding variable settings mimicking a study on functional outcome after stroke. We compared adjusted ordinal logistic regression (aOLR) and stratified Mann-Whitney measure of superiority (sMWS), and also used propensity scores to stratify the MWS (psMWS). For comparability, OLR estimates were transformed to a MWS. We report bias, the percentage of runs that produced a point estimate deviating by more than 0.05 points (point estimate variation), and the coverage probability.ResultsIn scenario 1, there was no bias in both sMWS and aOLR, with similar point estimate variation and coverage probabilities. In scenario 2, sMWS resulted in more bias (0.04 versus 0.00), and higher point estimate variation (41.6% versus 3.3%), whereas coverage probabilities were similar. In scenario 3, there was no bias in both methods, point estimate variation was higher in the sMWS (6.7%) versus aOLR (1.1%), and coverage probabilities were 0.98 (sMWS) versus 0.95 (aOLR). With psMWS, bias remained 0.00, with less point estimate variation (1.5%) and a coverage probability of 0.95.ConclusionsThe bias of both adjustment methods was similar in our stroke simulation scenario, and the higher point estimate variation in the MWS improved with propensity score based stratification. The stratified MWS is a valid alternative for adjusted OLR only when the ratio of number of strata versus number of observations is relatively low, but propensity score based stratification extends the application range of the MWS.
Educational differentials in domain specific physical activity by ethnicity, age, and gender: findings from over 44,000 participants in The UK Household Longitudinal Study (2013-2015)
Authors: Fluharty Meg E , Pinto Perira Snehal M , Benzeval Michaela , Hamer Mark , Jefferis Barbara , Griffiths Lucy , Cooper Rachel , Bann David 
Published: July 2019

AbstractBackgroundThe prevalence of overall physical inactivity remains high, particularly amongst socioeconomically disadvantaged groups. It is unclear however if such inequalities vary systematically by age, sex, or ethnicity, and if there are differing effects across physical activity (PA) domains.MethodsWe used data from a nationally representative survey of the UK, Understanding Society, with information on educational attainment (our indicator of socioeconomic position), PA and demographics collected in 2013-2015 (N= 44,903). Logistic regression analyses were conducted to test associations of education with three different PA domains (active travel, occupational and leisure time). To examine modification of the associations between education and physical activity in each domain by sex, age and ethnicity, we tested two-way interaction terms (education x ethnicity; education x sex; education x age).ResultsLower educational attainment was associated with higher active transportation and occupational physical activity, but lower weekly leisure-time activity. These associations were modified by sex, ethnicity, and age. For example, education-related differences in active travel were larger for females (difference in predicted probability of activity between highest and lowest educational groups: −10% in females, (95% CI: −11.9, 7.9) −3% in males (−4.8, −0.4). The education-related differences in occupational activity were larger among males −35% (−36.9, −32.4) than females −17% (−19.4, −15.0). Finally, education related differences in moderate to vigorous leisure time activity varied most substantially by ethnicity; for example, differences were 17% (16.2, 18.7) for White individuals compared with 6% (0.6, 11.6) for Black individuals.ConclusionsEducational differences in PA vary by domain, and are modified by age, sex, and ethnicity. A better understanding of physically inactive sub-groups may aid development of tailored interventions to increase activity levels and reduce health inequalities.
Reliability and validity of the UK Biobank cognitive tests
Authors: Fawns-Ritchie Chloe , Deary Ian J 
Published: July 2019

AbstractUK Biobank is a health resource with data from over 500,000 adults. The participants have been assessed on cognitive function since baseline. The cognitive tests in UK Biobank are brief and bespoke, and are administered without supervision on a touchscreen computer. Psychometric information on the tests is limited. The present study examined their concurrent validity and short-term test-retest reliability. A sample of 160 participants (mean age=62.59, SD=10.24) completed the UK Biobank cognitive assessment and a range of well-validated cognitive tests (‘reference tests’). Fifty-two participants returned 4 weeks later to repeat the UK Biobank tests. Correlations were calculated between UK Biobank tests and the reference tests. Four-week test-retest correlations were calculated for UK Biobank tests. UK Biobank cognitive tests showed a range of correlations with their respective reference tests, i.e. those tests that are thought to assess the same underlying cognitive ability (mean Pearson r=0.53, range=0.22 to 0.83, p≤.005). Four-week test-retest reliability of the UK Biobank tests were moderate-to-high (mean Pearson r=0.55, range=0.40 to 0.89, p≤.003). Despite the brief, non-standard nature of the UK Biobank cognitive tests, some showed substantial concurrent validity and test-retest reliability. These psychometric results provide currently-lacking information on the validity of the UK Biobank cognitive tests.
An Assessment of Transparency and Reproducibility-related Research Practices in Otolaryngology
Authors: Johnson Austin L. , Torgerson Trevor , Skinner Mason , Hamilton Tom , Tritz Daniel , Vassar Matt 
Published: July 2019

ABSTRACTIntroductionClinical research serves as the foundation for evidence-based patient care, and reproducibility of results is consequently critical. We sought to assess the transparency and reproducibility of research studies in otolaryngology by evaluating a random sample of publications in otolaryngology journals between 2014 and 2018.MethodsWe used the National Library of Medicine catalog to identify otolaryngology journals that met the inclusion criteria (available in the English language and indexed in MEDLINE). From these journals, we extracted a random sample of 300 publications using a PubMed search for records published between January 1, 2014, and December 31, 2018. Specific indicators of reproducible and transparent research practices were evaluated in a blinded, independent, and duplicate manner using a pilot-tested Google form.ResultsOur initial search returned 26,498 records, from which 300 were randomly selected for analysis. Of these 300 records, 286 met inclusion criteria and 14 did not. Among the empirical studies, 2% (95% CI, 0.4%-3.5%) of publications indicated that raw data were available, 0.6% (95% CI, 0.3%-1.6%) reported an analysis script, 5.3% (95% CI, 2.7%-7.8%) were linked to an accessible research protocol, and 3.9% (95% CI, 1.7%-6.1%) were preregistered. None of the publications had a clear statement claiming to replicate, or to be a replication of, another study.ConclusionsInadequate reproducibility practices exist in otolaryngology. Nearly all studies in our analysis lacked a data or material availability statement, did not link to an accessible protocol, and were not preregistered. Most studies were not available as open access. Taking steps to improve reproducibility would likely also improve patient care.
Collaborative Cross Mouse Populations as a Resource for the Study of Epilepsy
Authors: Gu Bin , Shorter John R. , Williams Lucy H. , Bell Timothy A. , Hock Pablo , Dalton Katherine A. , Pan Yiyun , Miller Darla R. , Shaw Ginger D. , Cooley Brian C. , Philpot Benjamin D. , de Villena Fernando Pardo-Manuel 
Published: July 2019

ABSTRACTEpilepsy is a neurological disorder with complex etiologies and genetic architecture. Animal models have a critical role in understanding the pathophysiology of epilepsy. Here we studied epilepsy utilizing a genetic reference population of Collaborative Cross (CC) mice with publicly available whole genome sequences. We measured multiple epilepsy traits in 35 CC strains, and we identified novel animal models that exhibit extreme outcomes in seizure susceptibility, seizure propagation, epileptogenesis, and sudden unexpected death in epilepsy. We performed QTL mapping in an F2 population and identified seven novel and one previously identified loci associated with seizure sensitivity. We combined whole genome sequence and hippocampal gene expression to pinpoint biologically plausible candidate genes and candidate variants associated with seizure sensitivity. These resources provide a powerful toolbox for studying complex features of seizures and for identifying genes associated with particular seizure outcomes, and hence will facilitate the development of new therapeutic targets for epilepsy.
CD4+Thimmunogenicity of theAscaris spp. secreted products
Authors: Ebner Friederike , Morrison Eliot , Bertazzon Miriam , Midha Ankur , Hartmann Susanne , Freund Christian , Álvaro-Benito Miguel 
Published: July 2019

SummaryAscaris spp. is a major health problem of humans and animals alike, and understanding the immunogenicity of its antigens is required for developing urgently needed vaccines. The parasite-secreted products represent the most relevant, yet highly complex (>250 proteins) antigens ofAscaris spp.as defining the pathogen-host interplay. We applied anin vitroantigen processing system coupled to quantitative proteomics to identify potential CD4+Thcell epitopes inAscaris suum-secreted products. This approach restricts the theoretical list of epitopes, based on affinity prediction, by a factor of ∼1200. More importantly, selection of 2 candidate peptides based on experimental evidence demonstrated the presence of epitope-reactive T cells inAscaris-specific T cell lines generated from healthy human individuals. Thus, this stringent work pipeline identifies a human haplotype-specific T cell epitope of a major human pathogen. The methodology described represents an easily adaptable platform for characterization of highly complex pathogenic antigens and their MHCII-restriction.
Discovering venom-derived drug candidates using differential gene expression
Authors: Romano Joseph D. , Li Hai , Napolitano Tanya , Realubit Ronald , Karan Charles , Holford Mandë , Tatonetti Nicholas P. 
Published: July 2019

AbstractVenoms are a diverse and complex group of natural toxins that have been adapted to treat many types of human disease, but rigorous computational approaches for discovering new therapeutic activities are scarce. We have designed and validated a new platform—named VenomSeq—to systematically identify putative associations between venoms and drugs/diseases via high-throughput transcriptomics and perturbational differential gene expression analysis. In this study, we describe the architecture of VenomSeq and its evaluation using the crude venoms from 25 diverse animal species and 9 purified teretoxin peptides. By integrating comparisons to public repositories of differential expression, associations between regulatory networks and disease, and existing knowledge of venom activity, we provide a number of new therapeutic hypotheses linking venoms to human diseases supported by multiple layers of preliminary evidence.
Processing of a single ribonucleotide embedded into DNA by human nucleotide excision repair and DNA polymerase η
Authors: Sassa Akira , Tada Haruto , Takeishi Ayuna , Harada Kaho , Suzuki Megumi , Tsuda Masataka , Sasanuma Hiroyuki , Takeda Shunichi , Sugasawa Kaoru , Yasui Manabu , Honma Masamitsu , Ura Kiyoe 
Published: July 2019

ABSTRACTDNA polymerases often incorporate non-canonical nucleotide, i.e., ribonucleoside triphosphates into the genomic DNA. Aberrant accumulation of ribonucleotides in the genome causes various cellular abnormalities. Here, we show the possible role of human nucleotide excision repair (NER) and DNA polymerase η (Pol η) in processing of a single ribonucleotide embedded into DNA. We found that the reconstituted NER system can excise the oxidized ribonucleotide on the plasmid DNA. Taken together with the evidence that Pol η accurately bypasses a ribonucleotide, i.e., riboguanosine (rG) or its oxidized derivative (8-oxo-rG) in vitro, we further assessed the mutagenic potential of the embedded ribonucleotide in human cells lacking NER or Pol η. A single rG on the supF reporter gene predominantly induced large deletion mutations. An embedded 8-oxo-rG caused base substitution mutations at the 3’-neighboring base rather than large deletions in wild-type cells. The disruption of XPA, an essential factor for NER, or Pol η leads to the increased mutant frequency of 8-oxo-rG. Furthermore, the frequency of 8-oxo-rG-mediated large deletions was increased by the loss of Pol η, but not XPA. Collectively, our results suggest that base oxidation of the embedded ribonucleotide enables processing of the ribonucleotide via alternative DNA repair and damage tolerance pathways.