bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulated transcription in chromosomal domains of midbrain dopamine neurons linked to schizophrenia.
Authors: Girdhar Kiran , Singh Swadha , Iskhakova Marina , Lambert Tova , Valada Aditi , Shokrian Neda , Evans Viviana , Bendl Jaroslav , Auluck Pavan , Marenco Stefano , Hoffman Gabriel , Roussos Panos , Akbarian Schahram 
Published: November 2024

Abstract

Ventral midbrain dopaminergic neurons are a key cell type for schizophrenia pathophysiology but information about cell type-specific genomic dysregulation in diseased brains is missing. We generated a unique midbrain functional genomics resource with 111 RNA-seq and 34 Hi-C chromosomal contact libraries for Nurr1+/NeuN + dopaminergic and their surrounding Nurr1-/NeuN- nuclei, collected from donors diagnosed with schizophrenia (SCZ) compared to neurotypical controls and to bipolar disorder (BD). Among the N = 340 dopamine neuron genes specifically dysregulated in SCZ were N = 104 downregulated, with selective enrichment for risk-associated synaptic plasticity and neuronal connectivity pathways and embedded within dopamine neuron-specific topologically associated chromosomal domains (TAD). Transcript-resolved analysis revealed 1,832 transcripts with altered expression in SCZ dopamine neurons, affecting key susceptibility genes such as the CNTNAP2, NRNX1, and SHANK2 neural cell adhesion molecules. This disease–associated transcriptomic remodeling in SCZ dopaminergic neurons was inversely correlated with corresponding transcriptome shifts in SCZ prefrontal cortex and striatum. Therefore, genomic dysregulation in the ventral midbrain of subjects diagnosed with SCZ selectively affects dopaminergic neurons, and includes a unilateral association of genetic risk with down-, but not upregulated transcription at the sites of highly organized chromosomal domains harboring neuron-specific genes with complex transcriptional architectures.
Multicomponent physical exercise programme among community-dwelling older adults who are users of home care services: A quasi-experimental pilot study
Authors: Ruiz-Fernández Aida , Irazusta Jon , Rezola-Pardo Chloe , Erezuma Unai Latorre , Goirigolzarri-Garaizar Andere , Ugalde-Aranzabal Nora , Espin Ander , Rodriguez-Larrad Ana 
Published: November 2024

Abstract

Objective
This quasi-experimental parallel pilot study aimed to analyse the feasibility and effectiveness of a multicomponent physical exercise programme (MPEP) among community-dwelling older adults who were users of home care services (HCS).
Methods
Forty-four participants were allocated to a MPEP group or a control (CON) group based on choice. Participants in the MPEP group performed individualised and progressive strength, balance and flexibility exercises three times per week for 16 weeks. One session was a group face-to-face session guided by a physical therapist, and the other two were individual home-based sessions following the Vivifrail programme. Feasibility was assessed via recruitment, adherence, safety and acceptability. Physical performance; nutritional, psycho-affective and cognitive status; and frailty were measured at baseline and after the intervention.
Results
Among the 604 eligible participants, 44 consented to participate. Completion rate was 7%. A total of 83% of participants attended face-to-face sessions with a compliance rate of 73%. Reported compliance with at-home sessions was 71%. No dangerous adverse events were reported. The participants reported an average satisfaction of 9 out of 10. Group-by-time interactions in favour of the MPEP group were found for the Short Physical Performance Battery (p=0.024) and Fried Frailty Scale (p=0.019). The MPEP group showed a decrease in the number of frail participants according to the Fried Frailty Scale (p=0.020) and the Short Physical Performance Battery (p=0.050). There were no other group-by-time interactions.
Conclusions
The MPEP is feasible and effective at improving physical performance and frailty status among community-dwelling older adults who are users of HCS (NCT05726214 in the ClinicalTrials.gov registry).
Revisiting Franco Moretti’s Concept and Practice of Literary Geography
Authors: Chen Shuping 
Published: November 2024

The research observed Franco Moretti, an influential Italian literary critic who had made significant contributions to the field of literary geography, a cross-disciplinary theory that utilized geographical methods to analyze and understand literary and cultural materials. Moretti’s approach to literary geography diverged from traditional and orthodox methods, offering a fresh perspective. The research applied a qualitative method with a closereading approach to evaluate and explore Moretti’s conceptualization and implementation of literary geography through a systematic three-step research process. The first step involved identifying the intellectual and theoretical sources of inspiration that have influenced Moretti’s work. The second step focused on illuminating Moretti’s innovative understanding and interpretation of literary geography as well as his integration of literary maps into the analysis of literature. Lastly, the research investigated two case studies to further exemplify Moretti’s practices in the field of literary geography. The first case study regarded Jane Austen’s fiction, where Moretti’s approach unveils Austen’s geographical imagination of Britain in her novels. The second one centers on Mary Mitford’s ‘Our Village’, where Moretti discovered the circular narrative pattern. By undertaking these three comprehensive steps, the research offers valuable insights into literary geography’s conceptual and methodological dimensions. Additionally, it endeavors to cultivate a deeper appreciation for the interdisciplinary nature of literary geography, thereby inspiring scholars and researchers to delve further into the intersections between geography, literature, and culture.
Analytical Evaluation of the WebSocket and WebTransport Protocols for the Class of SCADA Systems Operating Within the Intranet
Authors: Zvonareva G. A. , Smirnov A. S. 
Published: November 2024

The purpose of research. In recent years, web technologies have been actively used in many process control systems, including SCADA. Such systems allow continuous monitoring of the test object in real time. In this regard, there is a need for frequent data transmission for their further display. To solve this problem, network protocols are used, which continue to develop rapidly to this day.Materials and methods. SCADA systems are used in many industries: aviation, ground transportation, rocket and space technology, systems for receiving and processing telemetry information. Among them, a class of SCADA systems operating within the intranet stands out. This can be an internal network of the enterprise, a hangar or a small test stand for tracking the indexes of measuring equipment. Therefore, today, more than ever, open and publicly available materials are important for understanding modern network solutions that reduce the time spent on data transmission and their further visualization. Such information will be of particular value to students studying network and web technologies.Results. As a result of the research, taking into account the limitations of the SCADA system and network being developed, the time characteristics for the WebSocket and WebTransport protocols have been determined, and the most optimal solution for a typical load is proposed. In the presented paper, the issues of data transfer between the client and server parts for a class of SCADA systems operating within an intranet are considered. In this regard, it is proposed to consider the two most suitable solutions for this task. These are application layer network protocols of the OSI model: WebSocket and WebTransport. In addition, transport layer protocols (TCP and QUIC) are taken into account, since they can also affect time characteristics. Data transmission issues are considered in the context of the SCADA data display and monitoring system. The server part is developed using C#, and the client is developed using JavaScript. A number of experiments are carried out for boundary cases and typical loads, on the basis of which the dependence of data transmission time on their volume is determined.Conclusion. The approach given in the paper on collecting network traffic and analyzing time characteristics will also be useful as part of the educational process for teaching students in the courses “Computer Networks” and “Internet Technologies”.
Computational Screening of Novel Nitroimidazole Candidates: Targeting Key Enzymes of Oral Anaerobes for Anti-Parasitic Potential
Authors: Lanez Touhami , Lanez Maroua , Lanez Riad , Lanez Elhafnaoui , Talbi-Lanez Badia 
Published: November 2024

Background:
The study focuses on evaluating the parasitic potential of novel metronidazole
analogs using computational methods. Specifically, it aims to target key enzymes of oral anaerobes,
including UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA) of Fusobacterium nucleatum
and DNA topoisomerase (Topo) of Prevotella intermedia.

Objective:
The objective is to assess the pharmacokinetic and toxicity properties of 368 novel nitroimidazole
candidates through virtual screening. Additionally, the study aims to determine the binding
affinity of the most promising candidates with the target proteins through molecular docking
analyses.

Methods:
A combinatorial library of nitroimidazole candidates was constructed, and virtual screening
was performed. Molecular docking analyses were conducted to evaluate the binding affinity of selected
compounds with MurA and Topo. Further investigation involved molecular dynamic simulation
to assess the stability of the compounds within the active sites of MurA and Topo.

Results:
All selected compounds exhibited activity against both MurA and Topo. Among them,
Mnz11, Mnz12, and Mnz15 demonstrated the lowest binding free energies and IC50 values. Molecular
dynamic simulation indicated that these three compounds remained stable within the active sites
of MurA and Topo, with RMSD values consistently below 2Å. Additionally, the antibacterial potential
of the most potent compound, Mnz15, was evaluated against a series of oral microbes.

Conclusion:
The study concludes that the newly identified nitroimidazole candidates show promise
as anti-parasitic agents, based on their activity against key enzymes of oral anaerobes and their pharmacokinetic
properties evaluated through computational methods.
Decoding Glycobiomarkers in Non-Alcoholic Steatohepatitis (NASH) and Related Hepatocellular Carcinoma (HCC)
Authors: Bansal Savita , Burman Archana , Sen Aparajita , Vachher Meenakshi 
Published: November 2024

Abstract:
The incidence of Hepatocellular carcinoma (HCC) is rising at an alarming rate. It is
now the third leading cause of cancer deaths worldwide. Non-alcoholic fatty liver disease
(NAFLD) and its more aggressive form of non-alcoholic steatohepatitis (NASH) are emerging as
significant risk factors for liver cirrhosis and HCC. Post-translational modifications in proteins especially
glycosylation leading to the synthesis of glycoproteins have been implicated in carcinogenesis.
Dysregulated glycoproteins and aberrant glycosylation patterns might contribute to the
establishment of a protumorogenic environment in NAFLD/NASH patients leading to the establishment
of hepatocarcinogenesis. Understanding the molecular mechanisms underlying the
changes in glycosylation patterns of certain proteins would help in deciphering the role of glycoproteins
in liver cancer and develop novel prognostic and diagnostic markers and therapeutic
strategies for the successful treatment of HCC. Herein we discuss some important glycoproteins
and altered glycosylation patterns that can be employed as biomarkers for the early detection of
HCC in NASH and NAFLD patients.
Efficacy and Safety of a Unani Pharmacopeial Powder Formulation and Sharbat Banafsha in Women with Uncomplicated Urinary Tract Infection:
A Pilot Self-Control Clinical Trial
Authors: Sultana Arshiya , Rahman Khaleequr , Khaleeq khadija 
Published: November 2024

Background:
Antimicrobial resistance is a growing global concern, highlighting the
urgent need for effective non-antibiotic alternatives to treat urinary tract infections. Effortlessly
blending traditional Unani knowledge with modern scientific evaluation, this study highlights
the potential of Unani formulations in managing uncomplicated UTIs. This study's findings underscore
the importance of exploring and bridging the gap between Unani medicine and evidence-
based practices. This study investigates the efficacy and safety of an Unani Pharmacopeial
powder formulation and Sharbat Banafsha in treating women with uncomplicated urinary
tract infections (UTI).

Materials and Methods:
The pre and post-test pilot self-control clinical study involved 13 participants
with uUTI who received 3.5 g powder Unani formulation comprising Lagenaria
siceraria (Molina) Standl, Malva sylvestris L., Cucumis sativus L. and Cucumis melo L., Portulaca
oleracea L., and Adiantum capillus L. with Sharbat Banafsha (syrup of Viola odorata L.)
25 ml twice daily for 8 days. The primary outcomes were assessed using the urinary tract infection
symptoms assessment (UTISA) questionnaire, while secondary outcomes included urine
culture and sensitivity, as well as health-related quality of life (HRQoL) assessment using the
SF-12 health survey. The data was analyzed, and p<0.05 was considered significant.

Results:
The UTISA total score from 15.69±2.18 was reduced to 4.00±5.07 on day 9 with a significance
difference(p<0.001). On day 14, 84.61% of the population had negative cultures. The
total SF-12, PCS, and MCS scores showed significant differences from Day 0 to 60 (p>0.05).
No adverse effects were reported in both groups. HPLC analysis confirmed the presence of the
plant metabolite luteolin in both the powder and syrup.

Conclusion:
The data explores the effectiveness of Unani formulations for bacterial urinary
tract infections (UTI), showing no adverse effects while also improving the HRQoL of women.
These herbal products contain luteolin, a bioactive molecule with antimicrobial properties.

Clinical Trial Registration Number:
CTRI/2020/02/023404 dated 17/02/2020.
Network Pharmacology Analysis to Explore the Pharmacological Mechanism of Cang Er Zi Powder Against Allergic Rhinitis
Authors: Cui Qisheng , Liu Ping , He Huifang , Chen Cuiyin , Hou Xuemin , Xie Tao , Chen Weijie 
Published: November 2024

Background:
Allergic rhinitis (AR) is a leading public health problem with high prevalence,
but the therapies remain limited. Cang Er Zi Powder (CEZP), a Traditional Chinese
Medicine formula, has been used for the clinical treatment of chronic rhinitis and allergic rhinitis
in China for decades. However, the underlying mechanism is unclear.

Objective:
In this study, we aimed to clarify the pharmacological mechanism of CEZP on allergic
rhinitis

Methods:
The active ingredients of CEZP were screened in the TCMSP
(http://tcmspw.com/tcmsp.php) database. The targets related to “allergic rhinitis” were retrieved
from MALACARDS, TTD, and DisGeNET disease target databases. The active ingredients and
the candidate targets for AR were constructed and visualized using Cytoscape 3.7.2 software. The
underlying mechanism involved in the treatment of CP against AR was analyzed using the WEB-
based GEne SeT AnaLysis Toolkit. The effects of CEZP on levels of β-hexosaminidase, histamine,
interleukin (IL)-4, and tumor necrosis factor (TNF)-α on DNP-IgE/HSA-stimulated rat basophilic
leukemia cells were determined by enzyme-linked immunosorbent assay (ELISA) kits.

Results:
A total of 78 active ingredients in 9 Chinese herbs of CEZP and 90 target overlap targets
from CEZP and AR were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and
Genomes (KEGG) enrichment analysis suggested that the inflammation response and NF-κB signaling
pathway were responsible for the therapeutic targets of CEZP on AR, and CEZP could suppress
mast cell degranulation via Toll-like receptor (TLR) and NF-κb signaling pathway.

Conclusion:
Network pharmacology analysis and in vitro assays suggested that CEZP may exert
therapeutic effects on AR by inhibiting the NF-κB signaling pathways.
Discussion of “Thermal conductivity of porous building materials: An exploration of new challenges in fractal modelling solutions”
Authors: Janssen Hans 
Published: November 2024

In November 2023 this journal published “Thermal conductivity of porous building ma­terials: An exploration of new challenges in fractal modelling solutions”. That paper gauges four fractal building materials’ thermal conductivity models, concluding that fractal-geometry-based approa­ches appear “a promising method” as they “demonstra­te high reliability in repro­ducing experimental data”. This discussion of the pa­per aims to shine a different light on the potential of fractal thermal conductivity models. It shows that good agreement with experimental data usually ori­ginates from calibration of various “physical” factors comprised in these models, with the fitted numbers commonly deviating from physical rea­lity. Moreover, exemplary instances reveal that good agreement with experimental data is obtained des­pite misinterpretation of measured outcomes or critical defects in model development, or, exceptio­nally, due to fabrication of validation information. This discussion does hence not share the pa­per’s positive opinion on the prospects of fractal-geometry-ba­sed ther­­mal conductivity models, and advises caution instead.
Examining Whether Patient Portal and Video Visit Use Differs by Race and Ethnicity Among Older Adults in a US Integrated Health Care Delivery System: Cross-Sectional Electronic Health Record and Survey-Based Study
Authors: Gordon Nancy P , Yin Chelsea , Lo Joan C 
Published: November 2024

Background
Health care systems are increasingly encouraging patients to use patient portals and participate in video visits. However, there is limited information about how portal use differs among older adults.

Objective
This study aimed to understand how patient portal and video visit use differed by age, race, and ethnicity among older adult patients with access to the same digital health resources.

Methods
This cross-sectional study used electronic health record and survey data for adults aged 65 to 85 years who were members of a large Northern California health care delivery system throughout 2019 and 2020. The electronic health record cohort (N=471,152) included 320,686 White, 35,892 Black, 44,922 Latino, 20,786 Chinese, 28,732 Filipino, 8473 South Asian, 6716 Japanese, 2930 Vietnamese, and 2015 Korean adults. Racial and ethnic group and age group (65 to 75 years vs 76 to 85 years) differences in having a patient portal account by December 2020, the performance of 2 portal activities (sending ≥1 message to a clinician in 2019 or 2020 and viewing ≥1 laboratory test result in 2020), and having ≥1 video visit during 2020 were examined. Modified log-Poisson regression was used to examine prevalence ratios for portal and video visit use, comparing racial and ethnic groups to White adults and Asian ethnic groups to Chinese adults after adjusting for sex and age. Data from a 2020 member survey were used to compare internet use factors among 2867 White, 306 Black, 343 Latino, 225 Chinese, and 242 Filipino adults.

Results
Black, Latino, and Filipino adults were less likely to have a patient portal account than White adults, and Filipino adults were less likely to have a patient portal account than Chinese adults. Black, Latino, Filipino, Korean, Vietnamese, and South Asian adults were less likely to have sent messages and viewed test results than White adults, while Chinese and Japanese adults’ use of these features was similar to that of White adults. Filipino, Vietnamese, and Korean adults were less likely to have performed the aforementioned activities than Chinese adults. Video visit use was lower among Black and Latino adults and higher among Chinese and South Asian adults compared with White adults (aged 76 to 85 years) and lower among Filipino, Korean, and Vietnamese adults compared to Chinese adults. Survey data suggested that underlying differences in internet use may partially explain the lower use of messaging by Black, Latino, and Filipino adults compared with White and Chinese adults.

Conclusions
Patient portal and video visit use differed by race, ethnicity, and age group among older adult patients with access to the same patient portal. Internet use factors may contribute to these differences. Differences in patient portal and video visit use across Asian subgroups underscore the importance of disaggregating use data by Asian ethnicity.
Transformation of State Civil Apparatus Learning Task Administration Services through the J-SiLAKON Application
Authors: Fu’adah Washiqotul , Hidayat Rofiq 
Published: November 2024

The emergence of the Covid-19 pandemic and the progress of industry 4.0. The developments encourage the government to digitize public administration services. The Jember Regency Personnel and Human Resources Development Agency (BKSDM) therefore launched a web-based application named J-SiLAKON (Jember Online Personnel Services System) for study assignments to increase effectiveness and efficiency in providing services, and to encourage good governance. This research examines the effectiveness of the transformation of ASN learning task administration services through J-SiLAKON at the Jember Regency Education Office. By Using a qualitative case study approach, data were collected through semi-structured interviews, passive observation, and primary documentation involving J-SiLAKON operators and staff from the Teachers and Education Personnel Division. The analysis of data involved condensation, presentation, and conclusion drawing, with triangulation to ensure data validity. Results showed that J-SiLAKON is easy to learn, control, understand, and use, thus improving users' proficiency in performing their duties. It provided significant benefits, including faster work, improved employee performance, increased productivity, and more effective administrative services. Overall, the J-SiLAKON application improves the efficiency, productivity and transparency of ASN study assignment administration services at the Education Office, meeting user expectations and improving the performance of the Education office.