bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐Cell Detection and Photostimulation on a Microfluidic Chip Aided with Gold Nanorods
Authors: Zhu Yujie , Xu Hui , Wei Xunbin , He Hao 
Published: July 2019

AbstractGold nanorods (GNRs) can be easily designed and synthesized to respond to photons in the near infrared (NIR) band. The photostimulation by laser irradiation can be mediated and enhanced by GNRs to introduce localized damage to cells for photodynamic/photothermal therapy (PDT or PTT). In this study, we show that cells stained with GNRs can be detected and stimulated simultaneously by short flashes of femtosecond‐laser irradiation on a microfluidic system effectively. In the relatively high‐throughput cell flow, the two‐photon luminescence from GNRs can be excited and detected. The GNRs also mediate and enhance the transient photostimulation of the cells. After photostimulation, cells can remain alive, go to apoptosis, or necrosis, respectively. The stimulation effect is strongly dependent on the photon density and stimulation duration. We found the cells remain alive, go to apoptosis or necrosis, dependent on the GNR staining, the laser illumination pattern and duration. Hence, our system provides a simple and effective method for high‐throughput cell stimulation and analysis on chip. © 2019 International Society for Advancement of Cytometry
Mucilage‐bound sand reduces seed predation by ants but not by reducing apparency: a field test of 53 plant species
Authors: LoPresti E. F. , Pan V. , Goidell J. , Weber M. G. , Karban R. 
Published: July 2019

AbstractSeed mucilage, a coating on seeds or fruit that becomes slimy and sticky when wet, has evolved convergently many times across plants. One common consequence of having seed mucilage is that sand and dirt particles stick to wet seeds and remain tightly bound to the seed surface after the mucilage dries. Here, we test the hypothesis that a mucilage‐bound sand coating protects the seed from seed predators; either as a physical barrier or by reducing apparency of the seed (i.e., camouflage). We experimentally manipulated the sand coating on seeds of 53 plant species of 13 families and assayed the defensive benefit of the sand coating in feeding “depots” near harvester ant nests in California's Central Valley. Consistent with a defensive function, sand coating reduced ant predation on seeds in 48 of the 53 species examined. To test whether this striking benefit was due to reduced apparency, we conducted an addition experiment using flax seeds in which we factorially manipulated the color of both the background substrate and the sand coating, creating visually apparent and unapparent seeds. Our results did not support the reduced apparency hypothesis; seeds coated in background‐matched sand were removed at the same rate as seeds coated in unmatched sand. The defensive benefit of a sand coating was not well‐predicted by seed mass, entrapped sand mass, or sand mass scaled by seed mass. Together, our results demonstrate that seed mucilage is a phylogenetically widespread and effective seed defensive trait and point to the physical barrier, not reduced apparency, as a mechanism.
The Changes of the Volatile Compounds Derived from Lipid Oxidation of Boneless Dry‐Cured Hams During Processing
Authors: Zhang Jian , Pan Daodong , Zhou Guanghong , Wang Ying , Dang Yali , He Jun , Li Gang , Cao Jinxuan 
Published: July 2019

The generation of aroma during processing of boneless dry‐cured hams is investigated by evaluating neutral and acid lipases, phospholipase, lipoxygenase (LOX), thiobarbituric acid reactive substances (TBARS) values, fatty acid composition, and volatile substances derived from lipid oxidation (VCDFLO). It is found that moisture content, acid lipase, and phospholipase decreases in the whole processing, but sodium chloride content and TBARS increase. Neutral lipase, LOX, and total fatty acids increase from raw ham to the end of resting and decrease subsequently. 15 VCDFLO containing six ketones, three aldehydes, three hydrocarbons, one alcohol, one carboxylic acid, and one furan are identified. The total peak area of VCDFLO increases during the entire processing and indicates 39.76–40.75% of total volatile compounds. Hexanal, 1‐octen‐3‐ol, octanal, and nonanal are the major characteristic volatiles of boneless dry‐cured hams. Therefore, VCDFLO formation is associated with lipid‐related enzymes and helpful for the aroma.Practical Applications: The pork that has high intramuscular fat content is usually selected to produce boneless dry‐cured hams, because intramuscular phospholipids, and triglycerides are important precursors to produce aroma compounds. Exploring the formation mechanism of flavor associated with lipid oxidation will be beneficial for improving the quality of hams. Therefore, this study demonstrates the relationship between lipolysis‐oxidation and the generation of flavor compounds during the processing of boneless dry‐cured hams.This study demonstrates that thiobarbituric acid reactive substances (TBARS) values can reveal the changed trend of volatile compounds derived from lipid oxidation (VCDFLO) very well, and that lipid‐related enzymes plays an important role in the development of VCDFLO.
Pomegranate peel extract alters the microbiome in mice and dysbiosis caused by Citrobacter rodentium infection
Authors: George Nadja S. , Cheung Lumei , Luthria Devanand L. , Santin Monica , Dawson Harry D. , Bhagwat Arvind A. , Smith Allen D. 
Published: July 2019

AbstractTreatment of mice with a pomegranate peel extract (PPX) decreased the pathogenicity of Citrobacter rodentium (Cr) infections. Here, we investigate the effects of PPX on the microbiome of uninfected or Cr‐infected C3H/HeNCr mice by 16S rRNA gene sequencing. Mice were treated with water or PPX for 14 days, feces were collected, and then, the mice were infected with Cr and feces collected again at day 6 postinfection. DNA was isolated from the fecal samples and subjected to 16S rRNA gene sequencing to determine the microbial composition. Differences in the composition of the microbiome were observed for untreated and PPX‐treated mice with PPX mice having decreased diversity. PPX treatment decreased the Firmicutes/Bacteroidetes ratio by increasing Bacteroidetes and decreasing Firmicutes levels. The decrease in Firmicutes was driven by a large reduction in Lactobacillus. PPX treatment increased the abundance of Proteobacteria and Verrucomicrobiae and decreased Actinobacteria. The relative abundance of Cr reached 22% in water‐treated but only 5% in PPX‐treated infected mice. These results suggest that consumption of pomegranate polyphenols altered the microbiome, making it more resistant to displacement by infection with Cr, indicating that pomegranate polyphenols may mitigate the pathogenic effects of food‐borne bacterial pathogens.
Experimental Analysis for Thermal Performance of Heat Distribution System in hot BoP of 2 kW‐class SOFC
Authors: Kim Y. B. , Kim E. , Yoon J. , Song H. W. 
Published: July 2019

AbstractThis study designed and tested the components of hot balance of plant (BoP) included in a 2 kW‐class solid oxide fuel cell (SOFC) system and evaluated their thermal performance. A Pd/Pt catalytic combustor was fabricated as a component of hot BoP, and integrated hot BoP with an efficient structure was fabricated by calculating the heat transfer rates of the air preheater and steam generator through a theoretical design method. It was found that placement of the air preheater in front of the steam generator exhibited the most excellent heat transfer rate. Moreover, the heat transfer rates of the designed air preheater and steam generator exceeded the upper limit of the maximum possible heat transfer of the preheated air and the water. In experimental results, the thermal efficiency of the integrated system was 60% at the reference heat input and 70% at low heat input. The oxidizing flame of the catalyst was visualized, and the oxidation characteristics of the catalytic combustor were analyzed by measuring the concentration of the final discharged gas, that is, carbon monoxide (CO). This study helps to fabricate the integrated hot BoP system, as it provides design techniques and efficient system configuration.
Homologs of aquifex aeolicus protein‐only RNase P are not the major RNase P activities in the archaea haloferax volcanii and methanosarcina mazei
Authors: Schwarz Thandi S. , Wäber Nadine B. , Feyh Rebecca , Weidenbach Katrin , Schmitz Ruth A. , Marchfelder Anita , Hartmann Roland K. 
Published: July 2019

AbstractThe mature 5′‐ends of tRNAs are generated by RNase P in all domains of life. The ancient form of the enzyme is a ribonucleoprotein consisting of a catalytic RNA and one or more protein subunits. However, in the hyperthermophilic bacterium Aquifex aeolicus and close relatives, RNase P is a protein‐only enzyme consisting of a single type of polypeptide (Aq_880, ~23 kDa). In many archaea, homologs of Aq_880 were identified (termed HARPs for Homologs of Aquifex RNase P) in addition to the RNA‐based RNase P, raising the question about the functions of HARP and the classical RNase P in these archaea. Here we investigated HARPs from two euryarchaeotes, Haloferax volcanii and Methanosarcina mazei. Archaeal strains with HARP gene knockouts showed no growth phenotypes under standard conditions, temperature and salt stress (H. volcanii) or nitrogen deficiency (M. mazei). Recombinant H. volcanii and M. mazei HARPs were basically able to catalyse specific tRNA 5′‐end maturation in vitro. Furthermore, M. mazei HARP was able to rescue growth of an Escherichia coli RNase P depletion strain with comparable efficiency as Aq_880, while H. volcanii HARP was unable to do so. In conclusion, both archaeal HARPs showed the capacity (in at least one functional assay) to act as RNases P. However, the ease to obtain knockouts of the singular HARP genes and the lack of growth phenotypes upon HARP gene deletion contrasts with the findings that the canonical RNase P RNA gene cannot be deleted in H. volcanii, and a knockdown of RNase P RNA in H. volcanii results in severe tRNA processing defects. We conclude that archaeal HARPs do not make a major contribution to global tRNA 5′‐end maturation in archaea, but may well exert a specialised, yet unknown function in (t)RNA metabolism. © 2019 IUBMB Life, 2019 © 2019 IUBMB Life, 71(8):1109–1116, 2019
Measuring life cycles using binomial option pricing: The pharmaceutical industry
Authors: Beneda Nancy 
Published: July 2019

AbstractThis study examines the N computed using an American Binomial Option Pricing Model to address issues in evaluating firms which may be difficult to value such as new pharmaceutical firms. New pharmaceutical products can take up to 12 years to bring a new product to market. This study uses a sample of 15 pharmaceutical initial public offerings (IPOs) brought to market in 2,000. The current study uses a firm's excess market value as a measure of valuable growth opportunities. The study attempts to estimate how much remaining investment in research and development (R&D) is required for potential market success. The study solves for N in an American Binomial Option Pricing Model. The resulting N represents a number which when multiplied by R&D, results in an implied estimated amount of remaining investment in R&D, which the stock market has priced into the value of growth opportunities. The option valuation model uses the firm's excess market value as a call option on the firm's market value, and R&D times N, as the strike price. N is computed for each firm and year observation. The results of the current study suggest that the N, computed in the option‐pricing model, is negatively and highly associated with a firm's future performance and future productivity of investment in R&D.
Identification of flavonoids and chlorogenic acids in elm fruits from the genus Ulmus and their antioxidant activity
Authors: Gu Zhao‐Yu , Feng Cheng‐Yong , Li Shan‐Shan , Yin Dan‐Dan , Wu Qian , Zhang Ling , Wang Liang‐Sheng 
Published: July 2019

AbstractElm fruits were once an important food source in the years of famine. Research on the functional compounds in elm fruits was almost unavailable. In this study, we established an efficient high‐performance liquid chromatography method for the simultaneous separation of eight chlorogenic acids and 28 flavonoids in elm fruits for the first time. Total flavonoid contents ranged from 286 mg/100 g (Ulmus laciniata) to 1228 mg/100 g (U. pumila). High concentrations of rutin, quercetin 3‐O‐glucoside, and kaempferol derivatives were present in U. laevis, U. castaneifolia, and U. pumila, respectively. Furthermore, the fruit extracts of U. americana, U. castaneifolia, U. davidiana, and U. pumila showed higher antioxidant activity. These results suggest that fruits of these species can be used as bioresources for the extraction of the corresponding functional compounds. This work provides informative data and can be an important reference for future research on elm fruits as a renewed food resource.
Ultrasound Measurements of Frontal Horns and the Cavum Septi Pellucidi in Healthy Fetuses in the Second and Third Trimesters of Pregnancy
Authors: Ho Yoona , Herrero Tiffany , Aguinaldo Jennifer , Hahn Michael E. , Klisser Kristin , Ghassemi Neda , Alagh Amy , Hamlin Alyssa , Pretorius Miles , Horton Katelyn , Hull Andrew , Pretorius Dolores H. 
Published: July 2019

ObjectivesTo assess the visualization rate and size of the frontal horns (FHs) and cavum septi pellucidi (CSP) in healthy fetuses throughout pregnancy.MethodsAfter Institutional Review Board approval, 522 consecutive uncomplicated singleton pregnancies between 15 and 39 gestational weeks were enrolled in the study. Ultrasound measurements of the anterior horn width (AHW), center from the horn distance (CFHD), distance from the FHs to the CSP, and CSP width were retrospectively performed using axial transventricular or transcerebellar planes. Available maternal body mass indices were recorded.ResultsAt least 1 FH was seen in 78% of the cases. The mean AHW decreased over the second trimester and plateaued in the third trimester. The CFHD plateaued in the second trimester and increased in the third trimester. Downside FHs were generally larger than upside FHs. More FHs were measured in transventricular (69%) than transcerebellar (31%) planes. Frontal horns were seen with high, low, and no confidence in 57%, 21%, and 22% of cases, respectively. No‐confidence rates were 17% in the second trimester and 42% in the third trimester. The CSP was not visualized in 4% of cases; 15 of 19 cases of a nonvisualized CSP were scanned between 18 and 37 weeks. Mean body mass indices ± SDs were 27.6 ± 6.7 kg/m2 for the patients in cases of a visualized CSP and 32.4 ± 9.1 kg/m2 for the patients in cases of a nonvisualized CSP.ConclusionsNormative data for the fetal FH and CSP width were established. Frontal horns are more frequently seen on transventricular views and are difficult to confidently assess in the late third trimester. This study challenges previously reported data that the CSP is seen in 100% of cases from 18 to 37 weeks.
Predictive Value of Midtrimester Universal Cervical Length Screening Based on Parity
Authors: Rosenbloom Joshua I. , Raghuraman Nandini , Temming Lorene A. , Stout Molly J. , Tuuli Methodius G. , Dicke Jeffery M. , Macones George A. , Cahill Alison G. 
Published: July 2019

ObjectivesTo evaluate the effect of parity on performance characteristics of midtrimester cervical length (CL) in predicting spontaneous preterm birth (sPTB) before 37 weeks.MethodsThis was a retrospective cohort study of 13,508 women with no history of sPTB undergoing universal transvaginal CL screening at 17 to 23 weeks’ gestation from 2011 to 2016. Patients who declined screening or with unknown delivery outcomes were excluded. Areas under the receiver operator characteristic curves were used to assess and compare the predictive ability of CL screening for sPTB. The sensitivity, specificity, and positive and negative predictive values were estimated for specific CL cutoffs for prediction of sPTB.ResultsThere were 20,100 patients, of whom 2087 (10%) declined screening and 4505 (22%) did not meet inclusion criteria. Of the remaining 13,508 patients, 43% were nulliparous. The incidence rates of sPTB were 6.5% in nulliparas and 4.9% in multiparas (P < .001). The mean CLs were 39.9 mm in nulliparas and 41.8 mm in multiparas (P < .001), and those of the first percentiles were 19.0 mm in nulliparas and 24.0 mm in multiparas. Cervical length was significantly more predictive of sPTB in nulliparas (area under the curve, 0.67; 95% confidence interval, 0.63–0.70; versus 0.61, 95% confidence interval, 0.57–0.63; P = .008). At CL cutoffs of 10, 15, 20, and 25 mm or less, the sensitivity was lower in multiparas, and the specificity was comparable between the groups.ConclusionsMidtrimester CL is less predictive of sPTB in multiparas compared to nulliparas. The poor predictive ability, especially in multiparas, calls into question the value of universal CL screening in this population.
Nonlinear Optical Signatures of the Transition from Semiconductor to Semimetal in PtSe2
Authors: Wang Lei , Zhang Saifeng , McEvoy Niall , Sun Yi‐yang , Huang Jiawei , Xie Yafeng , Dong Ningning , Zhang Xiaoyan , Kislyakov Ivan M. , Nunzi Jean‐Michel , Zhang Long , Wang Jun 
Published: July 2019

AbstractThe demand for an ultrabroad optical material with a bandgap tunable from zero to at least 1–2 eV has been one of the driving forces for exploring new 2D materials since the emergence of graphene, transition metal dichalcogenides, and black phosphorus. As an ultra‐broadband 2D material with energy bandgap ranging from 0 to 1.2 eV, PtSe2 shows much better air stability than its analogue, black phosphorous. In this work, the superior nonlinear optical performance and ultrafast dynamics of layered PtSe2, and signatures of the transition from semiconductor to semimetal are systematically studied. Combining with rate equations, first‐principles calculation, and electrical measurements, a comprehensive understanding about the evolution of nonlinear absorption and carrier dynamics with increasing layer thickness is provided, indicating its promising potential in nanophotonic devices such as infrared detectors, optical switches, and saturable absorbers.
Copper‐catalyzed C‐H Bis‐nitration of 1‐Naphthylamide Derivatives with tert‐Butyl Nitrite as Nitro Source
Authors: Li Jizhen , Qin Zengxin , Zhang Changjing , Zhang Yingchao , Wen Chunxia 
Published: July 2019

AbstractA simple and atom‐economical protocol for bis‐nitration of 1‐naphthylamides was firstly discovered with tert‐butyl nitrite (TBN) as nitro source and catalyzed by Cu(OAc)2. Assisted by picolinamide direction, the C2,4‐H bis‐nitration could be achieved with excess amount of TBN at 50°C in HOAc. A radical pathway and single electron transfer process might be involved in the bis‐nitration process.
Shaeer’s Anti-Scarring Technique: A Preventive Measure Against Corporal Fibrosis Upon Explantation of Infected Penile Implants
Authors: Shaeer Osama , Abdel Rahman Islam Fathy Soliman , Shaeer Kamal 
Published: July 2019

Abstract

Introduction
In cases of explantation and delayed reimplantation of an infected penile prosthesis, the scarring that afflicts the corporal bodies renders reimplantation difficult and risky, with potential loss in penile size.

Aim
Mitomycin C is an antitumor, antibiotic agent with a potent antifibrotic action that can be used to limit corporal scarring following explantation with the aim of achieving easy and safe subsequent reimplantation, in addition to preserving penile size.

Methods
This was a prospective study involving 5 patients with infected penile prostheses who were referred to our tertiary implantation center. The infected prostheses were explanted, followed by corporal washout with antiseptics and antibiotics. Patients were rescrubbed and redraped. Mitomycin C, 10 mg in 250 cc saline, was instilled into the corpora cavernosa (125 cc each), avoiding extracavernous spilling and contact with corporotomy and skin edges. Corporotomy and skin edges were freshened and closed. Reimplantation was performed 10 to 12 weeks later.

Main Outcome Measure
We evaluated the ease of blunt dilatation upon reimplantation and success in implanting cylinders the same size as the ones explanted.

Results
We were able to dilate the corporal bodies with ease in all cases using blunt Hegar dilators. All cases received the same size implant as the one explanted, in terms of length and girth, with the exception of a case where the length was only 1 cm shorter.

Conclusions
Irrigation of the cavernous spaces with mitomycin C upon explantation of an infected penile prosthesis appears to ameliorate corporal scarring and keep the cavernous spaces open. On a larger scale, this approach could render the most feared complication of penile prosthesis implantation surgery much more manageable.
Quaking in their boots? Inaccurate perceptions of seismic hazard and public policy inaction
Authors: MOTTA MATTHEW , ROHRMAN ANDREW 
Published: July 2019

AbstractFew Americans demand that their local policy-makers take action to address the effects of large earthquakes, even in ‘high-risk’ areas. This poses an important political problem. If policy-makers do not perceive a mandate to prepare for catastrophe, certain areas of the country may be vulnerable to loss of life and economic productivity. Why do Americans not demand more from their policy-makers? We propose a simple answer – many Americans do not accurately appraise the likelihood that they will experience a major earthquake. In a unique survey of West Coast adults, we compared respondents’ perceived likelihood of experiencing a major earthquake to their actual geocoded hazard. We uncover a wide disconnect between actual and perceived earthquake hazard, even in areas where earthquakes are comparatively more common. Critically, and in contrast to previous public policy research, we show that threats in the physical environmentcanshape policy opinion, but only under certain circumstances. We show that accurate appraisals of hazard significantly increase the likelihood that respondents will support preventative local policy measures. Our results shed new light on the opinion dynamics of public attitudes toward natural disasters and ameliorative policy efforts and highlight the policy importance of communicating earthquake hazard to at-risk constituencies.
Improving Latino/a American students’ attitudes toward persons with disabilities and use of live theater
Authors: Diallo Abdoulaye , Braitewaite Jean , Mamboleo George , Tiwari Ashwini , Sharma Manisha 
Published: July 2019

AbstractThis study investigated the efficacy of a live theater performance intervention for enhancing the attitudes of pre-service rehabilitation students toward people with disabilities. A convenience sample of 54 undergraduate students of Latino/a descent (females = 33 and males = 21), age range 18 - 24 completed a pre-post design attitude change intervention. Data were collected on the students’ attitudes toward persons with disabilities, using the scale of attitudes towards disabled persons, and gladness, using a liker scale. Multivariate analysis of covariance was used to analyze the data. Results indicated the live theater performance intervention was more effective in providing positive emotion of gladness and in improving the attitudes of the students towards PWDs compared to simple print media.