bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA-203 Inhibits Long Noncoding RNA HOTAIR and Regulates Tumorigenesis through Epithelial-to-mesenchymal Transition Pathway in Renal Cell Carcinoma
Authors: Dasgupta Pritha , Kulkarni Priyanka , Majid Shahana , Shahryari Varahram , Hashimoto Yutaka , Bhat Nadeem S. , Shiina Marisa , Deng Guoren , Saini Sharanjot , Tabatabai Z. Laura , Yamamura Soichiro , Tanaka Yuichiro , Dahiya Rajvir 
Published: February 2018

Abstract
This study aims to investigate the role of miR-203–HOTAIR interaction in the suppression of renal cell carcinoma (RCC). We employed series of in vitro assays such as proliferation, invasion, migration, and colony formation along with in vivo tumor xenograft model. Profiling of miR-203 and HOTAIR expression revealed that miR-203 was significantly underexpressed, whereas HOTAIR was overexpressed in RCC cell lines and clinical specimens compared with normal cell line and tissue. Both miR-203 and HOTAIR expression significantly distinguished malignant from normal tissues and significantly correlated with clinicopathologic characteristics of patients. Overexpression of miR-203 significantly inhibited proliferation, migration, and invasion with an induction of apoptosis and cell-cycle arrest. However, HOTAIR suppression resulted in the similar functional effects in the same RCC cell lines. In silico, RNA-22 algorithm showed a binding site for miR-203 in HOTAIR. We observed a direct interaction between miR-203 and HOTAIR by RNA-immunoprecipitation (RIP) and luciferase reporter assays. We show that miR-203–HOTAIR interaction resulted in the inhibition of epithelial-to-mesenchymal transition (EMT) and metastatic genes as indicated by induction of key metastasis-suppressing proteins E-cadherin, claudin (epithelial markers), and PTEN along with induction of tumor suppressor genes p21 and p27. A significant decrease in vimentin (mesenchymal marker), KLF4, and Nanog (stemness markers) was also observed. This is the first report demonstrating miR-203–mediated regulation of HOTAIR induces tumor suppressor effects in RCC by regulating EMT and metastatic pathway genes. Thus, the study suggests that therapeutic regulation of HOTAIR by miR-203 overexpression may provide an opportunity to regulate RCC growth and metastasis. Mol Cancer Ther; 17(5); 1061–9. ©2018 AACR.
PORTRAIT (Patient-Centered Outcomes Related to Treatment Practices in Peripheral Arterial Disease: Investigating Trajectories)
Authors: Smolderen Kim G. , Gosch Kensey , Patel Manesh , Jones W. Schuyler , Hirsch Alan T. , Beltrame John , Fitridge Rob , Shishehbor Mehdi H. , Denollet Johan , Vriens Patrick , Heyligers Jan , Stone, MEd Nancy , Aronow Herbert , Abbott J. Dawn , Labrosciano Clementine , Tutein-Nolthenius Rudolf , A. Spertus John 
Published: February 2018

Background:
Health status outcomes, including symptoms, functional status, and quality of life, are critically important outcomes from patients’ perspectives. The PORTRAIT study (Patient-Centered Outcomes Related to Treatment Practices in Peripheral Arterial Disease: Investigating Trajectories) was designed to prospectively define health status outcomes and examine associations between patients’ characteristics and care to these outcomes among those presenting with new-onset or worsened claudication.

Methods and Results:
PORTRAIT screened 3637 patients with an abnormal ankle-brachial index and new, or worsened, claudication symptoms from 16 peripheral arterial disease (PAD) specialty clinics in the United States, the Netherlands, and Australia between June 2, 2011, and December 3, 2015. Of the 1608 eligible patients, 1275 (79%) were enrolled. Before treatment, patients were interviewed to obtain their demographics, PAD symptoms and health status, psychosocial characteristics, preferences for shared decision-making, socioeconomic, and cardiovascular risk factors. Patients’ medical history, comorbidities, and PAD diagnostic information were abstracted from patients’ medical records. Serial information about patients’ health status, psychosocial, and lifestyle factors was collected at 3, 6, and 12 months by a core laboratory. Follow-up rates ranged from 84.2% to 91%. Clinical follow-up for PAD-related hospitalizations and major cardiovascular events is ongoing.

Conclusions:
PORTRAIT systematically collected serial PAD-specific health status data as a foundation for risk stratification, comparative effectiveness studies, and clinicians’ adherence to quality-based performance measures.

Clinical Trial Registration:

URL:
https://www.clinicaltrials.gov
. Unique identifier: NCT01419080.
Female control of reproductive behaviour in the platypus (Ornithorhynchus anatinus), with notes on female competition for mating
Authors: Thomas Jessica L. , Parrott Marissa L. , Handasyde Kathrine A. , Temple-Smith Peter 
Published: February 2018

AbstractOpportunities for studying platypus courtship and mating behaviours in the wild are limited due to the nocturnal and cryptic nature of this species. We report on platypus courtship and mating behaviour from a successful breeding program at Healesville Sanctuary, Victoria, in which platypuses were held as either breeding pairs or trios over seven years. Behaviour was recorded daily on infrared cameras resulting in over 80,000 h of footage that was analysed for activity periods, and courtship and mating behaviours including non-contact and contact courtship, mating and avoidance. Our aims were to describe and quantify courtship and mating interactions between males and females, and to determine if either sex controlled the initiation and continuation of the behaviours. From our observations, we describe a new courtship behaviour, non-contact courtship, which constituted the majority of all mating season interactions between males and females. The time between first and last appearance of a courtship and mating behaviour was 41.0 ± 6.6 days, with the females showing behavioural receptivity for 29.6 ± 5.1 days. Female platypuses used three evasive strategies in relation to approaches by males: avoidance, flight and resistance. Females controlled the duration of 79% of encounters using resistance. For the first time, two females were seen competing with each other over access to the male platypus in their enclosure and for nesting material. Time investment in courtship and mating behaviours was a poor indicator of receptivity and breeding success, and we suggest that breeding failure is more likely to be associated with failure of fertilisation, nest building, embryonic development or incubation. We describe how female platypuses demonstrate evasiveness and control of courtship and mating behaviours, and the importance of providing these opportunities in captivity to promote successful breeding.
A new species of the hermit crab genus Diogenes (Crustacea: Decapoda: Diogenidae) from the coast of KwaZulu-Natal, South Africa
Authors: LANDSCHOFF JANNES , RAHAYU DWI LISTYO 
Published: February 2018

A new diogenid hermit crab with bright-white chelae, Diogenes albimanus n. sp., is described and illustrated, based on a single ovigerous female collected from KwaZulu-Natal, South Africa. The new species is compared to morphologically similar species, in particular to D. holthuisi and D. dorotheae, from which it is distinguishable by the shape and armature of the telson; and also to the superficially-similar South African D. extricatus, from which it differs by having the unarmed dorsal margins of the propodi and meri of the pereopods, as opposed to being armed with rows of strong spines. The unusually white chelae are similar in colour to those of D. takedai, but the coloration of all other parts is different in these two species. High quality macro photographs, a high-resolution microCT (μCT) scan of the whole animal, and the reference to the repository of the molecular barcode of the new species are provided.
Thunderstorm Characteristics during the Ontario Winter Lake-Effect Systems Project
Authors: Steiger Scott M. , Kranz Tyler , Letcher Theodore W. 
Published: February 2018

AbstractThe Ontario Winter Lake-Effect Systems (OWLeS) field campaign during the winter season of 2013/14 provided unprecedented data with regard to the structure and behavior of long-lake-axis-parallel (LLAP) lake-effect storms. One of the interesting characteristics of LLAP storm bands is their ability to initiate lightning. The OWLeS datasets provide an opportunity to examine more thoroughly the kinematics and microphysics of lake-effect thunder-snowstorms than ever before. The OWLeS facilities and field personnel observed six lake-effect thunderstorms during December–January 2013/14. Most of them produced very little lightning (fewer than six cloud-to-ground strokes or intracloud pulses recorded by the National Lightning Detection Network). The 7 January 2014 storm had over 50 strokes and pulses, however, which resulted in 20 flashes over a 6-h period (0630–1230 UTC), making it the most electrically active storm during the field campaign. Relative to the 18 December 2013 storm, which only had three flashes, the 7 January 2014 case had a deeper boundary layer and greater instability. Also, 45% of the lightning during the 7 January storm was likely due to flashes initiated by wind turbines or other man-made antennas, along with all of the lightning observed during 18 December. No lightning was documented over Lake Ontario, the primary source of instability for these storms.
Dynamics of a Quasi-Stationary Jet along the Subarctic Front in the North Pacific Ocean (the Western Isoguchi Jet): An Ideal Two-Layer Model
Authors: Miyama Toru , Mitsudera Humio , Nishigaki Hajime , Furue Ryo 
Published: February 2018

ABSTRACTThe dynamics of a quasi-stationary jet along the Subarctic Front in the North Pacific Ocean (the Western Isoguchi Jet) were investigated using an idealized two-layer model. The experiments suggested that a seafloor topography, which is 500 m high, produces a jet along its eastern flank. The formation mechanism of the jet can be explained via baroclinic Rossby wave characteristics. Baroclinic Rossby waves propagate along characteristic curves, which are significantly distorted by anticyclonic barotropic flow on the seafloor topography. A baroclinic surface jet is formed where a characteristic curve originating in the subtropical gyre and one originating in the subpolar gyre meet because the pycnocline depth varies discontinuously at this location. The barotropic flow on the seafloor topography is induced by eddies.
Implications of Regulatory Drought for farmer Use of Climate Information in the Klamath Basin
Authors: VanderMolen Kristin , Horangic Alexandra 
Published: February 2018

Abstract
Despite the risk of climate variability to agriculture, farmer use of climate information in agricultural decision-making generally remains low. Research has suggested that where farmers already have robust “repertoires” of decision-making resources adapted to some degree of climate variability, such new information may simply factor less saliently. This study asks whether farmer use of climate information increases under the occurrence of more extreme climatic events for which those repertoires lack referent—in this case, severe hydrological and related regulatory drought in the Klamath basin. Semistructured interviews with key informants of Klamath basin agriculture indicate a marked increase in farmer use of climate and climate-related information since the onset of drought in 2001. What information farmers utilize, however, depends on whether it retains its predictive and explanatory value under both types of drought. Findings highlight the need for consideration of coproduction approaches to the development of climate information if it is to serve farmers where the extremity of climate events produces changes not only in availability of but also in access to key agricultural resources.
Caveat emptor: Folate in unipolar depressive illness, a systematic review and meta-analysis
Authors: Roberts Emmert , Carter Ben , Young Allan H 
Published: February 2018

Background: International guideline recommendations for the use of folate and its derivatives in the treatment of unipolar depressive disorders are confused and contradictory, perhaps reflecting wide variations in the underpinning evidence base. Introduction: We discuss differing methods of evidence synthesis in the formulation of international guideline recommendations. As an example we evaluated the efficacy of folate and its derivatives in unipolar depression via systematic review and meta-analysis. Methods: We searched Medline, EMBASE, PsychInfo and CENTRAL from database inception until 1 May 2017 for randomised controlled trials. We included trials that evaluated folate or its derivatives as monotherapy or to augment antidepressant therapy compared with placebo in patients with unipolar depressive illness. Standardised mean differences were used and studies were introduced as subgroups to explain the heterogeneity. Quality was assessed using the Grading of Recommendations Assessment, Development and Evaluation. Results: No trials report on folate or methylfolate versus placebo as a monotherapeutic option. Only when the evidence was restricted to folate at a dose of <5 mg/day or methylfolate at a dose of 15 mg once daily as an adjunct to selective serotonin reuptake inhibitor therapy was there a significant benefit compared with placebo. All evidence was graded as low or very low quality for each outcome. Discussion: Whilst previous guidelines on the treatment of unipolar depression have either avoided this topic entirely, or made recommendations on the basis of cherry-picked evidence, this review is the first to attempt to provide clinically useful recommendations based on comprehensive, current randomised placebo-controlled data. We invite discussion of the review and its recommendations, which are based on the limited evidence regarding folate formulation delivered and appropriate dosage.
Arthroscopic Surgery or Physical Therapy for Patients With Femoroacetabular Impingement Syndrome: A Randomized Controlled Trial With 2-Year Follow-up
Authors: Mansell Nancy S. , Rhon Daniel I. , Meyer John , Slevin John M. , Marchant Bryant G. 
Published: February 2018

Background: Arthroscopic hip surgery has risen 18-fold in the past decade; however, there is a dearth of clinical trials comparing surgery with nonoperative management. Purpose: To determine the comparative effectiveness of surgery and physical therapy for femoroacetabular impingement syndrome. Study Design: Randomized controlled trial; Level of evidence, 1. Methods: Patients were recruited from a large military hospital after referral to the orthopaedic surgery clinic and were eligible for surgery. Of 104 eligible patients, 80 elected to participate, and the majority were active-duty service members (91.3%). No patients withdrew because of adverse events. The authors randomly selected patients to undergo either arthroscopic hip surgery (surgery group) or physical therapy (rehabilitation group). Patients in the rehabilitation group began a 12-session supervised clinic program within 3 weeks, and patients in the surgery group were scheduled for the next available surgery at a mean of 4 months after enrollment. Patient-reported outcomes of pain, disability, and perception of improvement over a 2-year period were collected. The primary outcome was the Hip Outcome Score (HOS; range, 0-100 [lower scores indicating greater disability]; 2 subscales: activities of daily living and sport). Secondary measures included the International Hip Outcome Tool (iHOT-33), Global Rating of Change (GRC), and return to work at 2 years. The primary analysis was on patients within their original randomization group. Results: Statistically significant improvements were seen in both groups on the HOS and iHOT-33, but the mean difference was not significant between the groups at 2 years (HOS activities of daily living, 3.8 [95% CI, –6.0 to 13.6]; HOS sport, 1.8 [95% CI, –11.2 to 14.7]; iHOT-33, 6.3 [95% CI, –6.1 to 18.7]). The median GRC across all patients was that they “felt about the same” (GRC = 0). Two patients assigned to the surgery group did not undergo surgery, and 28 patients in the rehabilitation group ended up undergoing surgery. A sensitivity analysis of “actual surgery” to “no surgery” did not change the outcome. Twenty (33.3%) patients who underwent surgery and 4 (33.3%) who did not undergo surgery were medically separated from military service at 2 years. Conclusion: There was no significant difference between the groups at 2 years. Most patients perceived little to no change in status at 2 years, and one-third of military patients were not medically fit for duty at 2 years. Limitations include a single hospital, a single surgeon, and a high rate of crossover. Registration: NCT01993615 ( ClinicalTrials.gov identifier)
Biomechanical Evaluation of a Single- Versus Double-Tunnel Coracoclavicular Ligament Reconstruction With Acromioclavicular Stabilization for Acromioclavicular Joint Injuries
Authors: Banffy Michael B. , Uquillas Carlos , Neumann Julie A. , ElAttrache Neal S. 
Published: February 2018

Background: An anatomic double-tunnel (DT) reconstruction technique has been widely adopted to reconstruct the ruptured coracoclavicular (CC) ligaments seen with high-grade acromioclavicular (AC) joint injuries. However, the anatomic DT reconstruction has been associated with the risk of clavicle fractures, which may be problematic, particularly for contact athletes. Purpose/Hypothesis: The purpose was to compare a single-tunnel (ST) CC ligament reconstruction for AC joint injuries with the native state as well as with the more established anatomic DT CC ligament reconstruction. The hypothesis was that ST CC ligament reconstruction would demonstrate biomechanical properties similar to those of the native state and the DT CC ligament reconstruction. Study Design: Controlled laboratory study. Methods: Eighteen fresh-frozen human cadaveric shoulders (9 matched pairs) with mean ± SD age of 55.5 ± 8.5 years underwent biomechanical testing. One specimen of each matched pair underwent a ST CC ligament reconstruction and the second, a DT CC ligament reconstruction. The ST and DT CC ligament reconstruction techniques involved a 5-mm distal clavicle excision, avoided coracoid drilling, and utilized a 3.0-mm suture anchor to fix the excess lateral limb to reconstruct the superior AC joint capsule. The ST CC ligament reconstruction technique additionally included a 1.3-mm suture tape to help avoid a sawing effect, as well as a dog-bone button over the clavicular tunnel to increase stability of the construct. All specimens were tested to 70 N in 3 directions (superior, anterior, and posterior) in the intact and reconstructed states. The ultimate load, yield load, stiffness, and mode of failure of the reconstructed specimens were tested. Results: There were no significant differences in translation at 70 N in the superior ( P = .31), anterior ( P = .56), and posterior ( P = .35) directions between the ST CC ligament reconstruction and the intact state. The ultimate load to failure, yield load, and stiffness in the ST and DT groups were also not significantly different. There were no distal clavicle fractures in load-to-failure testing in the ST or DT group. Conclusion: In this biomechanical study, ST CC ligament reconstruction demonstrates biomechanical properties comparable to the intact state. Additionally, use of the ST CC ligament reconstruction shows biomechanical properties similar to the DT CC ligament reconstruction technique while theoretically posing less risk of clavicle fracture. Clinical Relevance: This study suggests that the ST CC ligament reconstruction has biomechanical properties equivalent to the DT CC ligament reconstruction with less theoretical risk of clavicle fracture.
A Randomized Clinical Trial to Evaluate Attached Hamstring Anterior Cruciate Ligament Graft Maturity With Magnetic Resonance Imaging
Authors: Liu Shaohua , Li Hong , Tao Hongyue , Sun Yaying , Chen Shiyi , Chen Jiwu 
Published: February 2018

Background: The hamstring tendons are commonly harvested for anterior cruciate ligament (ACL) reconstruction with detachment of the tibial insertion. Retaining the insertion may help to preserve vascularity and viability of the graft and bypass the stages of avascular necrosis and revascularization, which might be beneficial to graft maturity. Purpose: To investigate and compare graft maturity by magnetic resonance imaging (MRI) after ACL reconstruction with preservation or detachment of hamstring tendon tibial insertion at up to 2 years. Study Design: Randomized controlled trial; Level of evidence, 2. Methods: Forty-five patients (age range, 18-45 years) undergoing isolated ACL reconstruction with hamstring tendon were enrolled and randomized to 2 groups. The tibial insertion of the hamstring tendon was preserved in the study group (n = 21) and detached in the control group (n = 24). Patients had follow-up at 3, 6, 12, and 24 months, which consisted of the following: (1) clinical examination and (2) MRI evaluation of graft signal intensity based on signal/noise quotient (SNQ) values. Finally, 18 patients in the study group and 19 in the control group received full follow-up evaluation (ie, at all 4 time points). Results: All knees acquired full range of motion at 24 months without significant laxity. At each time point, the KT-1000 arthrometer revealed no significant difference between groups; the clinical scores significantly improved in both groups, although the difference between groups was not significant. In the control group, the SNQ value increased from 3 months, peaked at 6 months, and then decreased (3 months, 21.4 ± 12.7; 6 months, 25.6 ± 12; 12 months, 18.3 ± 7.7; 24 months, 15.3 ± 6.3). However, the insertion-preserved graft in the study group maintained relatively lower and unchanged signal intensity throughout all time points (3 months, 15.0 ± 11.2; 6 months, 14.9 ± 6.3; 12 months, 12.6 ± 7.0; 24 months, 14.6 ± 7.0). Between groups, there was no significant difference at 3 or 24 months ( P = .11 and .75, respectively), while the SNQ values were significantly lower in the study group versus the control group at 6 and 12 months ( P = .002 and .02, respectively). Conclusion: The insertion-detached hamstring tendon grafts underwent a significantly increasing change in signal intensity during the first 2 years after ACL reconstruction, while the insertion-preserved grafts kept a relatively lower and unchanged signal intensity. The difference was most significant at 6 and 12 months postoperatively.