bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic analysis of admixture and hybrid patterns of Populus hopeiensis and P. tomentosa
Authors: Wang Dongsheng , Wang Zhaoshan , Kang Xiangyang , Zhang Jianguo 
Published: March 2019

AbstractHybridization and introgression have resulted in reticulate evolution within the genusPopulus. Consequently, the origin and evolutionary history of some hybrids has become blurred.P.hopeiensisandP.tomentosaare endemic to China, and there is still controversy about their origin. We employ phylogeny, Bayesian estimation of admixture, and approximate Bayesian computation to investigate their origin with 10 nuclear DNA and 6 cpDNA regions. The combined evidences firmly support the hypothesis that they are hybrids and dominated by F1s.P.hopeiensiswas generated via hybridization between the paternal speciesP.albaand maternal speciesP.davidiana. Surprisingly,P.tomentosawas divided into two genetic types with different maternal parents.P.adenopodahybridized withP.albadirectly to generate the first genetic type (mb1) and hybridized withP.davidianafollowed byP.albato generate the second (mb2). In both genetic types,P.albaacted as the male parent. The maternal parent wasP.adenopodaandP.davidianafor mb1 and mb2, respectively. Hybridization not only generated these hybrids but also resulted in a unidirectional gene flow fromP.davidianatoP.adenopoda. ThePopulusspecies have maintained a delicate balance between their genetic integrity and gene exchange.
The Familial dementia gene ITM2b/BRI2 facilitates glutamate transmission via both presynaptic and postsynaptic mechanisms
Authors: Yao Wen , Yin Tao , Tambini Marc D. , D’Adamio Luciano 
Published: March 2019

AbstractMutations in the Integral membrane protein 2B (ITM2b/BRI2) gene, which codes for a protein called BRI2, cause familial British and Danish dementia (FBD and FDD). Loss of BRI2 function and/or accumulation of amyloidogenic mutant BRI2-derived peptides have been proposed to mediate FDD and FBD pathogenesis by impairing synaptic Long-term potentiation (LTP). However, the precise site and nature of the synaptic dysfunction remain unknown. Here we use a genetic approach to inactivate Itm2b in either presynaptic (CA3), postsynaptic (CA1) or both (CA3 + CA1) neurons of the hippocampal Schaeffer-collateral pathway in both female and male mice. We show that after CA3 + CA1 Itm2b inactivation, spontaneous glutamate release and AMPAR-mediated responses are decreased, while short-term synaptic facilitation is increased. Moreover, AMPAR-mediated responses are decreased after postsynaptic but not presynaptic deletion of Itm2b. In contrast, the probability of spontaneous glutamate release is decreased, while short-term synaptic facilitation is increased, primarily after presynaptic deletion of Itm2b. Collectively, these results indicate a dual physiological role of Itm2b in the regulation of excitatory synaptic transmission at both presynaptic termini and postsynaptic termini and suggest that presynaptic and postsynaptic dysfunctions may be a pathogenic event leading to dementia and neurodegeneration in FDD and FBD.
Tracing recombinant bovine somatotropin ab(use) through transcriptomics: the potential of bovine somatic cells in a multi-dose longitudinal study
Authors: Lamas Alexandre , Regal Patricia , Vázquez Beatriz , Miranda José Manuel , Cepeda Alberto , Franco Carlos Manuel 
Published: March 2019

AbstractIn the European Union, the use of recombinant bovine somatotropin (rbST) in dairy cattle is forbidden. Monitoring rbST (ab)use by its direct detection in animal matrices still remains a challenging task. New monitoring methods based on indirect detection of the substance are necessary. A new transcriptomic system based on the use of high-throughput real-time PCR in combination with somatic cells was developed to control rbST administration in dairy animals. A total of nine cows, separated into control and rbST-treated groups, were included in the study. A subcutaneous injection containing 500 mg of rbST was administered to the treated group every 14 days, up to a total of 12 doses. Milk somatic cells (MSCs) were sampled from each animal at different time points throughout 8 months of study. It was possible to obtain the transcriptomic profile of 18 genes in MSCs of rbST-treated and control groups, and using univariate and multivariate statistical analysis control and treated animals were discriminated. The transcription of CCND1, IGF-1R, TNF and IL-1β genes resulted strongly influenced by rbST treatment. The combination of MSCs, transcriptomic tools and statistical analysis has allowed the selection of four genes as potential biomarkers that could be used in a transcriptomic panel for monitoring rbST administration in cows.
Prospectively reinstated memory drives conscious access of matching visual input
Authors: Gayet Surya , Moorselaar Dirk van , Olivers Christian N. L. , Paffen Chris L. E. , Stigchel Stefan Van der 
Published: March 2019

AbstractMaintaining information in visual working memory (VWM) biases attentional selection of concurrent visual input, by favoring VWM-matching over VWM-mismatching visual input. Recently, it was shown that this bias disappears when the same item is memorized on consecutive occasions (as memoranda presumably transit from VWM to long-term memory), but reemerges when observers anticipate to memorize a novel item on a subsequent trial. Here, we aimed to conceptually replicate and extend this intriguing finding, by investigating whether prospectively reinstated memory drives conscious access of memory-matching visual input. We measured the time it took for participants to detect interocularly suppressed target stimuli, which were either from the same color category as a concurrently memorized color or not. Our results showed that the advantage of memory-matching targets in overcoming suppression progresses non-monotonically across consecutive memorizations of the same color (‘repetitions’): the advantage for memory-matching visual input initially declined to asymptote, before being fully revived on the last repetition. This revival was not observed in a control experiment in which targets were not interocularly suppressed. The results suggest that, as observers anticipate to memorize a novel item imminently, VWM usage is prospectively reinstated, causing memory-matching visual input to gain accelerated access to consciousness again.
New Therapeutic Approach for Targeting Hippo Signalling Pathway
Authors: Dominguez-Berrocal Leticia , Cirri Erica , Zhang Xiguang , Andrini Laura , Marin Gustavo H. , Lebel-Binay Sophie , Rebollo Angelita 
Published: March 2019

AbstractNuclear localization signals are short amino acid sequences that target proteins for nuclear import. In this manuscript, we have generated a chimeric tri-functional peptide composed of a cell penetrating peptide (CPP), a nuclear localization sequence and an interfering peptide blocking the interaction between TEAD and YAP, two transcription factors involved in the Hippo signalling pathway, whose deregulation is related to several types of cancer. We have validated the cell penetration and nuclear localization by flow cytometry and fluorescence microscopy and shown that the new generated peptide displays an apoptotic effect in tumor cell lines thanks to the specific nuclear delivery of the cargo, which targets a protein/protein interaction in the nucleus. In addition, the peptide has an anti-tumoral effect in vivo in xenograft models of breast cancer. The chimeric peptide designed in the current study shows encouraging prospects for developing nuclear anti- neoplastic drugs.
Integrated transcriptome and in vitro analysis revealed anti-proliferative effect of citral in human stomach cancer through apoptosis
Authors: Balusamy Sri Renukadevi , Ramani Sivasubramanian , Natarajan Sathishkumar , Kim Yeon Ju , Perumalsamy Haribalan 
Published: March 2019

AbstractCancer is the second leading cause of death globally, particularly stomach cancer is third most common causes of cancer death worldwide. Citral possesses anti-tumor activity in various cancer cell lines, However its effect toward stomach cancer and its mechanism of action is have yet to be elucidated. The goal of the present study is to elucidate the role of citral in stomach cancer using transcriptome and in vitro approaches. We performed transcriptome analysis using RNA-seq and explored its capability to persuade apoptosis in AGS human stomach cancer cell lines in vitro. Furthermore, the enrichment and KEGG pathway results suggested that there are several genes involved to induce apoptosis pathway. Furthermore, our study also demonstrated that citral arrested colony formation and migration of cancer cells significantly than that of untreated cells. RNA-seq revealed a total of 125 million trimmed reads obtained from both control and citral treated groups respectively. A total number of 612 differentially expressed genes (DEGs) were identified which includes 216 genes up-regulated and 396 genes down-regulated genes after treatment. The enrichment analysis identified DEGs genes from transcriptome libraries including cell death, cell cycle, apoptosis and cell growth. The present study showed the significant inhibition effect upon citral by regulating various genes involved in signaling pathways, inhibits metastasis, colony formation and induced apoptosis both in silico and in vitro.
Elucidation of in vitro cellular steps induced by antitumor treatment with plasma-activated medium
Authors: Chauvin Julie , Gibot Laure , Griseti Elena , Golzio Muriel , Rols Marie-Pierre , Merbahi Nofel , Vicendo Patricia 
Published: March 2019

AbstractNumerous studies have reported cold atmospheric plasma cytotoxic activities in various cancer cell lines, either by direct exposure to non-thermal plasma or indirectly by activating a medium (plasma-activated medium, PAM) prior to cell treatment. We suggested the use of in vitro 3D tumor model spheroids to determine the potential of PAM for cancer therapy at the tissue scale, especially in human tumor tissue. This work aimed to better understand the effect of PAM on human colorectal tumor spheroids by describing the in vitro-induced-cell death kinetics and associated mechanisms to further improve its therapeutic potential. Tumor spheroid growth was delayed depending on contact time with PAM. Medium osmolarity was increased by activation with low temperature Helium plasma jet but it did not fully explain the observed growth delay. PAM impaired tumor cell viability through intracellular ATP depletion, leading within hours to both cell apoptosis and necrosis as well as mitochondrial oxidative stress. When successive treatments were spaced over time, cumulative effects on the growth delay of spheroids were observed. Taken together, these results demonstrated that plasma-activated liquids may represent a novel and efficient therapeutic method for the treatment of tumors, especially when successive treatments are applied.
Photoacoustic F-Mode imaging for scale specific contrast in biological systems
Authors: Moore Michael J. , Hysi Eno , Fadhel Muhannad N. , El-Rass Suzan , Xiao Yongliang , Wen Xiao-Yan , Kolios Michael C. 
Published: March 2019

AbstractIn photoacoustic (PA) imaging, time domain reconstruction techniques are the current gold standard for image formation. While these techniques provide high-resolution spatial maps of optical absorption, they neglect the structural information encoded in the frequency domain of the broadband PA signals. In this work, we introduce a frequency domain technique for PA image formation, termed F-Mode. By leveraging information contained in the frequency content of PA signals, F-Mode can be used to generate images with scale-specific contrast. To demonstrate the robustness of our technique, we apply F-Mode to datasets acquired using both PA tomography and PA microscopy systems, utilizing linear array and single-element transducers with central frequencies ranging from 40–400 MHz. Here we show that the technique can be used to: differentiate between vessels and microspheres of different size in phantoms, enhance visualization of organelles in cultured cells, and selectively display single blood vessels in vivo in zebrafish larvae.
Association between interleukin-10 gene rs1800896 polymorphism and diabetic retinopathy in a Chinese Han population
Authors: Liu Lun , Zheng Jie , Xu Yajing , Gao Jian , Fan Lingling , Xu Dong 
Published: March 2019

Abstract
The levels of cytokines, inflammatory cells, and angiogenic factors increase following diabetic retinopathy (DR), and the association between interleukin-10 (IL-10) gene rs1800896 polymorphism (IL-10 -1082G/A polymorphism) and DR in different populations has been extensively studied. However, the findings are conflicting, and there is no relevant research in Chinese subjects. Therefore, this case–control study involving 327 cases (type 2 diabetes patients with proliferative DR (PDR)) and 461 controls (type 2 diabetes patients without DR) was conducted to address the relationship between IL-10 gene rs1800896 polymorphism and risk of PDR in Chinese population. Genotyping was performed using PCR-based restriction fragment length polymorphism (PCR-RFLP) assay. It was found that GG genotype or G allele of the IL-10 gene rs1800896 polymorphism was associated with decreased risk for PDR. In conclusion, IL-10 gene rs1800896 polymorphism decreases the risk of PDR.
Editorial
Authors: Jung Britta 
Published: March 2019

Sehr geehrte Kolleginnen und Kollegen!Eine gesunde Ernährung, Bewegung und Sport spielen eine entscheidende Rolle, wenn es um Fitness, Reduktion des Kariesrisikos und damit insgesamt um die Gesundheit unserer Kinder geht. Doch aktuelle Daten zeigen, dass 15,4% der Kinder in Deutschland zwischen 3 und 17 Jahren übergewichtig sind und weitere 5,9% der Kinder an Adipositas leiden. Die Ursachen liegen vielfach in dem übermäßigen Zuckerkonsum bzw. übermäßigem Verzehr von Kohlenhydraten und damit an der nicht ausgewogenen Ernährungsweise.
Schlafmedizin in Deutschland
Authors: Young Peter , Penzel Thomas 
Published: March 2019

ZusammenfassungDie Schlafmedizin ist ein Querschnittsfach. Die schlafmedizinischen Erkrankungen werden als Schlafstörungen bezeichnet und sind durch die International Classification of Sleep Disorders 3rd Edition (ICDS-3) definiert. Das obstruktive Schlafapnoe Syndrom (OSAS) und die Ein- und Durchschlafstörungen (Insomnien) stellen die Gruppen der häufigsten Schlafstörungen dar. Zu den Schlafstörungen im engeren neurologischen Sinn zählen die Gruppe der Hypersomnolenz zentralen Ursprungs, die motorischen Störungen und die Parasomnien. Schlafmedizinische Diagnostik schließt immer eine schlafbezogene Anamnese, eine körperliche Untersuchung und häufig auch technische Untersuchungsmethoden wie die Aktimetrie, die Polygrafie und/oder die Polysomnografie mit ein. Die Polysomnografie ist die im Schlaflabor stattfindende Untersuchung.Durch die zunehmende Ökonomisierung der Medizin steht die Schlafmedizin vor dem Problem, dass die gemäß Akkreditierungsrichtlinien der Deutschen Gesellschaft für Schlafforschung und Schlafmedizin (DGSM) personalintensive Diagnostik im DRG-System unterfinanziert ist. Es findet deswegen eine zunehmende Verlagerung von stationärer schlafmedizinsicher Versorgung in Richtung ambulanter Versorgungsstrukturen statt. Besonders die neurologischen Schlafstörungen aus der Gruppe der Hypersomnolenz zentralen Ursprungs (z. B. die Narkolepsie) sind in der ambulanten Versorgung nicht ausreichend diagnostizierbar und behandelbar. Vor dem Hintergrund könnte in Zukunft ein abgestuftes System der Versorgung je nach Schwere und Art der Schlafstörung sinnvoll sein.
Vogt-Koyanagi-Harada Disease and Birdshot Retinochoroidopathy, Similarities and Differences: A Glimpse into the Clinicopathology of Stromal Choroiditis, a Perspective and a Review
Authors: Elahi Sina , Herbort Carl 
Published: March 2019

AbstractThe purpose of this work was to give a comprehensive and updated review on two primary stromal choroiditis entities, Vogt-Koyanagi-Harada disease (VKH) and birdshot retinochoroiditis (BRC). Their appraisal has become much more precise thanks to new investigational methods, such as indocyanine green angiography (ICGA) and enhanced depth imaging optical coherence tomography (EDI-OCT), which give substantially improved imaging access to the choroid. In this review, we focus on the crucial changes brought by this progress in the understanding, diagnosis, and management of these disorders. Application of these methods makes it possible to reach an early diagnosis, therefore allowing early treatment, which has led to a profound improvement in outcomes when compared to previous management.
Fernbehandlung aus psychotherapeutischer Sicht
Authors: Munz Dietrich , Jansen Alessa , Böhmig Carolin 
Published: March 2019

ZusammenfassungEs steht mittlerweile eine Vielzahl von digitalen Anwendungen zur Verfügung, die die psychotherapeutische Behandlung ergänzen und die Versorgung psychisch kranker Menschen verbessern können, sie können sie jedoch nicht ersetzen. Dazu gehören beispielsweise digitale Anwendungen zur Videokommunikation, die Psychotherapeuten und Patienten die Möglichkeit bieten, auch über Entfernung miteinander zu kommunizieren. Zudem stehen internetbasierte Interventionen wie Internet(behandlungs-)programme und Gesundheits-Apps für die Prävention und Behandlung psychischer Erkrankungen zur Verfügung. Aufgezeigt wird im Folgenden, welche Chancen und Risiken mit dem Einsatz digitaler Anwendungen bei psychischen Erkrankungen verbunden sein können. Damit digitale Anwendungen zu einer Verbesserung der psychotherapeutischen Versorgung beitragen können, müssen sie genauso sorgfältig durchgeführt werden wie eine Behandlung im unmittelbaren Kontakt. Dazu gehört, dass Diagnose, Indikationsstellung und Aufklärung grundsätzlich in unmittelbarem Kontakt zwischen Psychotherapeut und Patient erfolgen müssen. Dargestellt ist außerdem, welche gesetzlichen Rahmenbedingungen aktuell den Einsatz digitaler Anwendungen in der psychotherapeutischen Versorgung regeln.
Potenziale und Herausforderungen von E-Mental-Health-Interventionen in der Versorgung psychischer Störungen
Authors: Köhnen Moritz , Dirmaier Jörg , Härter Martin 
Published: March 2019

ZusammenfassungPsychische Störungen sind weit verbreitet und führen zu bedeutenden Beeinträchtigungen auf individueller und gesellschaftlicher Ebene. Trotz der starken Krankheitslast und des vergleichsweisen gut ausgebauten Versorgungssystems in Deutschland werden psychische Störungen nur unzureichend versorgt. Auch tragen Zugangsbarrieren wie etwa lange Wartezeiten auf ambulante Behandlungsplätze dazu bei, dass Gesundheitsdienstleistungen nur verzögert oder gar nicht durch Betroffene in Anspruch genommen werden. E-Mental-Health-Interventionen gelten als vielversprechende Optionen, um Zugangsbarrieren abzubauen und Versorgungslücken, z. B. durch eine Unterversorgung im ländlichen Raum, zu schließen. Sie können zeit- und ortsunabhängig angeboten werden und auch Menschen in Regionen mit geringer Versorgungsdichte oder Menschen mit körperlichen Beeinträchtigungen, die in ihrer Mobilität eingeschränkt sind, erreichen. Trotz des großen Potenzials von E-Mental-Health-Interventionen bestehen zahlreiche Herausforderungen, z. B. in Bezug auf den Datenschutz und die Präzisierung hinsichtlich begrifflicher, konzeptioneller und evaluativer Aspekte des sehr breiten E-(Mental)-Health-Sektors. Diese können zum einen hinderlich für die Implementierung in der Routineversorgung sein und zum anderen die Akzeptanz der Interventionen bei Behandlern und Nutzern beeinflussen. Der Überblicksbeitrag beschreibt und diskutiert Potenziale, Herausforderungen und den Präzisierungsbedarf von E-Mental-Health-Interventionen im Kontext der Versorgung psychischer Störungen.
A Case of Massive Hepatic Infarction in a Patient with HELLP Syndrome
Authors: Morgan Jessica , Della Torre Micaela , Whelan Anna , Rodriguez Sophia , DiGiovanni Laura 
Published: March 2019

Background Hepatic infarction is an exceedingly rare complication of hemolysis, elevated liver enzymes, and low platelets syndrome. Few cases have been described in the medical literature and the true incidence remains unknown. It can lead to fulminant liver failure, liver transplant, or death if not promptly addressed.
Case Report A 22-year-old primigravida presented with right upper quadrant and epigastric pain at 28 weeks' gestation. She had severely elevated blood pressures requiring intravenous antihypertensives as well as proteinuria, thrombocytopenia, and mild transaminitis. Within 6 hours of admission, her rapidly rising liver function tests (LFTs) necessitated urgent delivery by primary cesarean section. Her liver enzymes continued to rapidly worsen postoperatively and immediate postpartum computed tomography of the abdomen and pelvis revealed massive hepatic infarction, 11 × 10 × 15 cm, of the right lobe of the liver. Her transaminases peaked at alanine transferase of 2,863 IU/L and aspartate transferase of 2,732 IU/L. She received supportive multidisciplinary intensive care, and LFTs returned to normal by postoperative day 20.
Conclusion Hepatic infarction is an extraordinarily rare complication of pre-eclampsia. Early recognition and prompt multidisciplinary management are vital to prevent catastrophic bleeding, hepatic failure, and death.
Management of Helicobacter pylori in Patients with Immune Thrombocytopenia
Authors: Vanegas Yenny Alejandra Moreno , Vishnu Prakash 
Published: March 2019

AbstractThere is an association between Helicobacter pylori infection and immune thrombocytopenia (ITP), and few studies have suggested that eradicative treatment of H. pylori infection may improve platelet counts in patients with ITP. Conventional treatments for ITP include immunosuppressive agents, and more recently thrombopoietic agents. However, based on clinical reports of association between H. pylori and ITP, several medical societies increasingly suggest detection and eradication of H. pylori as a treatment for ITP. In this article, we reappraise recent medical literature to determine the effectiveness of platelet response after treatment of H. pylori infection in patients with ITP. We searched two online databases (MEDLINE and Google Scholar) for full articles published between January 2008 and May 2018, and found a total of 11 studies that presented data and outcomes of treatment of H. pylori infection in ITP patients. All the studies administered triple therapy (amoxicillin 500 mg, clarithromycin 250 mg and a proton-pump inhibitor each given twice daily for either 7- or 14-day course) for eradication of H. pylori. Median overall platelet response ranged from 27 to 69.2% with a complete response rate ranging from 0 to 65.4% and a partial response rate ranging from 0 to 29.4%. Although there is variability in the effectiveness between different populations, it appears to be of benefit to ITP patients with concomitant H. pylori infection when treated with triple therapy. However, further studies to understand the pathogenesis of H. pylori-associated ITP is necessary for the development of new therapeutic approaches for ITP.
The effect of size on structure and stress in grained films
Authors: Chocyk Dariusz , Zientarski Tomasz 
Published: March 2019

Molecular dynamics simulations are carried out to study the evolution of stress during deposition of atoms on the (001) plane of an FCC crystal, using rectangular simulation box with x– y periodic boundary conditions. The system consists of grains and boundaries. In the current study, we consider different values of the grain-to-grain distances and different relative sizes of deposited atoms. The structure was determined by the kinematical scattering theory. The main factors influencing the stress in thin film systems are creating of dislocation and changing the crystalline structure in grains. The deposited atoms can significantly contribute to the total stress. Depending on their size, atoms contribute compressive or tensile stresses to the total stress. This paper is part of a Thematic Issue on The Crystallographic Aspects of Metallic Alloys.