bluuebunny/crossref_metadata_2025_split huggingface dataset:
PB2037 CHANGES IN ERYTHROPOIESIS IN MALARIA‐INDUCED ANEMIA
Authors: Pêgo A.C. , Lima I. , Martins G. , Sá Pereira I. , Martins A.C. , Kapitão A. , Nai A. , Camaschella C. , Silvestri L. , Gozzelino R. 
Published: June 2019

Background:Severe malarial anemia (SMA) is a complication developed in the course of Plasmodium infection, which is often associated with increased rates of malaria‐related morbidity and mortality. Possibly linked to a dysregulated immune response and unbalanced production of inflammatory mediators, one of the main features of SMA involves an impaired and/or ineffective erythropoiesis, which seems to critically contribute to the high number of deaths due to malaria infection. Erythropoiesis is regulated by the hormone erythropoietin, which upon binding to its receptor, activates a signaling cascade that leads to an increased consumption of iron used for the production of red blood cells.Aims:To gain new insights into the pathogenesis of malaria infection, our study aims to assess how erythropoiesis changes in mice exposed to different strains of Plasmodium parasites, known to cause severe forms of malaria, and/or in response to multiple re‐infections.Methods:Flow cytometry analyses were used to show the complementarity between the bone marrow and spleen in regulating the erythropoietic response in infected‐mice and its correlation with parasite load.Results:The interplay between this phenomenon and the expression of genes regulating iron metabolism was also analyzed, showing a strict association with the severity the disease. The extent of immune response activation upon exposure to Plasmodium infection was also evaluated and linked to the degree of induced SMA.Summary/Conclusion:The results obtained demonstrated that malaria infection significantly alters the erythropoietic process. In particular, we observed an early response of the spleen, as to compensate the loss of an appropriate bone marrow erythropoiesis during the infection.
PB2038 EVOLUATION OF CLINICAL MANIFESTATION IN FEBRILE NEUTROPENIC PATIENTS
Authors: Bekoz H.S. , Kaya Z. , Mert A. 
Published: June 2019

Background:Neutropenia is one of the most life‐threatening and significant complication of intensive chemotherapy treatments. Febrile neutropenia is the major cause of mortality and morbidity in these patients (1).Aims:The aim of this study, was to evaluate the epidemiology and the surveillance factors in febrile neutropenic patients retrospectively.Methods:This study was undertaken in the our university from December 2014 to December 2017. Blood culture was studied by Bact‐Alert automated system.Antibiotic susceptibility tests were Kirby Baver Discus Fusion Method.Results:A total of 195 patients, 400 febrile neutropenic attacks were assessed.During FEN episodes, 178 of the patients (45%) had piperacillin‐tazobactam, 145(45%) had meropenem, 195(49%) had teicoplanin and 17(4%) had cefepime.In our study, microbiologically documented infection was 31%, clinically documanted infection was 33%, and responding to empirical antibiotic infection was 43%.imageIn our study, 83 blood culture positivity were studied for resistance, 33 (40%) of them had ESBL and 19 (23%) had carbapenemase‐producing microorganisms.imageSummary/Conclusion:For the management and determining empirical antibiotic policies, it is important to identify their pathogenic microorganisms, to determine their antimicrobial susceptibility for improving survival rates in FEN process. In our study, infections foci, culture positivity rates, isolated microorganisms consistent with the rates reported in the literature.
PB2043 UPPER AND LOWER GASTROINTESTINAL ENDOSCOPY FINDINGS IN PATIENTS WITH ISOLATED FERRITIN DEFICIENCY
Authors: Bahadir A. , Sağ E. , Çakır M. 
Published: June 2019

Background:Iron deficiency without anemia is the most common nutritional disorder in children. Iron balancing is provided by intestinal absorption and iron transfer mechanisms. The most common causes of anemia are chronic blood loss from the gastrointestinal tract (peptic ulcer, hemorrhoids, parasites), inadequate dietary intake, gynecological problems (menorrhagia), decreased gastrointestinal mucosal absorption (celiac disease), Helicobacter pylori (Hp) infection and autoimmune gastritis. In the literature, there are not many studies about iron deficiency without decreasing hemoglobin (Hg) levels in the early period of iron deficiency.Aims:In our study, we aimed to evaluate the lower and upper endoscopic findings of patients with normal Hg levels and low ferritin levels.Methods:A total of 2391 patients aged between 0‐18 years and had normal Hg levels were included into the study performing upper and lower gastrointestinal system (GIS) endoscopy by the pediatric gastroenterology‐hepatology department between 2009 and 2018. The patients were divided into two groups as Hb level was normal, ferritin level below 15 mg / dl (group 1; n = 549) and Hb level was normal, ferritin level was above 15 mg / dl (group 2; n = 1143). In group 1, upper GIS endoscopy was performed to 520 patients, lower GIS endoscopy to 56 patients. In Group 2, upper GI endoscopy was performed to 1095 patients and lower GI endoscopy to 120 patients. All endoscopy results of the patients were evaluated and compared.Results:The most common complaint was abdominal pain in both groups (63%, 67.4%, respectively). And the most common endoscopic findings in both groups were reflux esophagitis in the esophagus (9.6%, 9.1%, respectively, p>0.05), gastritis in the stomach (32.1%, 37.9%, respectively, p > 0.05). Hp gastritis was the second most common endoscopic finding in group 1. When the both groups were compared, there was a significant difference between group for Hp gastritis (24%, 27.6%, respectively, p = 0.002). In duodenum findings, celiac disease was higher in group 1 and there was a significant statistically between group for celiac disease (6%, 2.1%, respectively, p = 0.000). In colonoscopic examination, lymphoid nodular hyperplasia and polyp were higher in group 1 and there were statistically significant between groups (14.3%, 5%, and 10.7%, 3.3% respectively, p = 0.03, 0.04).Summary/Conclusion:In the literature, generally the studies are about anemia and upper GIS findings. In our study, the patients with decreased ferritin and normal Hg levels and undergoing upper and lower GIS endoscopy were investigated. The risk of Hp gastritis and celiac disease was found to be increased in patients with ferritin deficiency without anemia (group 1). In addition, lymphoid noduler hyperplasia and polyps were also caused ferritin deficiency.
PB2056 ORBITAL LYMPHOMA: CLINICAL AND EPIDEMIOLOGICAL PATTERNS WITHIN THE UNITED STATES
Authors: khalafAllah M. , gouda M. 
Published: June 2019

Background:Lymphomas are relatively common tumors that may affect the orbit through tissue infiltration. However, data available about tumors possibly originating in orbit (also called orbital lymphoma) are scarce.Aims:In this study, we aimed to spotlight on this type of malignancy using the using data from American National Cancer Institute, Surveillance, Epidemiology, and End Results (SEER) Program.MethodsWe used SEER∗Stat version 8.3.5, Microsoft Excel, as well as SPSS IBM SPSS Statistics for Windows, Version 25.0 for data analysis. In SEER∗Stat, we used the (Incidence ‐ SEER 18 Regs Research Data + Hurricane Katrina Impacted Louisiana Cases, Nov 2017 Sub (1973‐2015 varying)) database to obtain data. We included cases diagnosed with lymphoma between 2006 and 2015 at site related to orbital cavity C69.0‐C69.9.Results:A total of 2340 cases were identified with a median age of 65.2. Females and patients of white race represented the majority of the analyzed cohort (n = 1249, 53.4%; n = 1846, 78.9%, respectively). Age‐adjusted Incidence rate was 3 per 10,000. 5‐years observed survival was 82% (95% CI: 79.8% ‐ 84%) while the 5‐years relative survival was 94.3% (95% CI: 91.3% ‐ 96.2%). The most common pathology of studied cases was extranodal marginal zone lymphoma of mucosal‐associated lymphoid tissue (MALT) (n = 1310, 56%) [Table 1]. Median survival was not reached at the study cutoff (Figure 1).Summary/Conclusion:Orbital lymphoma has an incidence rate of 3 per 10,000. These tumors are more likely to occur in old age, females, and white race. Survival data are relatively good with the 5‐years relative survival being 94.3%.image
PB2063 HAIRY CELL VARIANT–LABORATORY FINDINGS OF A CASE SERIES
Authors: Chereghini A. , Gonclaves M. , Daniel D. , Silva R. , Maekawa Y. , Muramoto F. , Tamashiro N. , Takao T. , Sandes A. , Silva M. 
Published: June 2019

Background:Hairy cell leukemias are rare mature B‐cell neoplasms that morphologically present cytoplasmatic projections. According to the most recent World Health Organization Classification, they can be Classic (HCL) or Variant (HCLv). These two diseases present important clinical and laboratory differences. HCLv is the rarest of them with an incidence of less than 1 case:1.000.000 inhabitants/year. Unlike HCL, HCLv generally presents with elevated blood counts, there is no monocytopenia and the abnormal cells present an identifiable nucleolus. HCLv cells do not express CD25 or present BRAF mutation mutations, findings that are almost universally present in HCL.Aims:The aim of this study was to describe the laboratory findings of all HCLv cases diagnosed at Grupo Fleury from 2015 to 2018.MethodsA retrospective descriptive study of the laboratory findings of all HCLv cases diagnosed at Grupo Fleury from 2015 to 2018. Complete blood counts were obtained with a Sysmex XE 5000 counter and submitted to morphology review. Blood or bone marrow immunophenotypic prophiles were obtained using an 8‐color FACS Canto II cytometer for acquisition and Inifinicyt for analysis. Antibodies against CD3, CD4, CD5, CD8, CD10, CD11c, CD19, CD20, CD22, CD23, CD25, CD38, CD45, CD56, CD79b, CD103, CD123, CD200, KAPPA, LAMBDA, FMC7, IgM, IgD, IgG and IgA were used.Results:7 cases were diagnosed during this period (0.04% of all of suspected oncological cases submitted immunophenotyping). Median age was 86 years old (range: 58‐89), and 3 were males. Median CBC values were [median (range)]: hemoglobin = 10.1 g/dL (8.8‐12.7); lymphocytes = 2.4 × 109/L (1.4‐16.5); monocytes = 0.53 × 109/L (0.2‐3.1); platelets = 91 × 109/L (76‐97, and an outlier of 1.106 × 109/L). No cases presented monocytopenia Flow cytometry (5 of peripheral blood and 2 of bone marrow aspirate), demonstrated universal positivity for CD19, CD20, FMC‐7, CD103, and CD11c (partial expression in one case). No case demonstrated CD5, CD10, CD25, CD38 or CD123. Of note, CD200 expression was negative or mild in all cases. Five cases were IgM+/IgD+, one IgM+, and the other was IgG+. Two demonstrated kappa expression and 5 were lambda light chain restricted. Karyotype was normal in all of the 3 cases analyzed.Summary/Conclusion:Flow cytometry, along with morphology, was the main sources of diagnosis, as no specific genetic alteration is known. The presence of CD103 and CD11c in the absence of CD123 and CD25 suggest HCLv, excluding classical HCL. CD200, classically strongly expressed in classical HCL was negative or only weakly expressed in HCLv and may help on diagnosis.
PB2064 USE OF RNASCOPE TECHNOLOGY TO DETERMINE STAT‐3 EXPRESSION IN HUMAN DIFFUSE LARGE B‐CELL LYMPHOMA
Authors: Tamma R. , Gaudio F. , Ingravallo G. , Albano F. , Perrone T. , Annese T. , Ruggieri S. , Maiorano E. , Specchia G. , Ribatti D. 
Published: June 2019

Background:Diffuse large B‐cell lymphoma (DLBCL) is the most common and one of the most heterogeneous lymphomas. Therefore, it is critical to further stratify cases of DLBCL into biologically similar and clinically meaningful subgroups, which will not only guide prognostic assessment and facilitate therapeutic decisions, but also stimulate further research to understand the pathogenesis and develop potential novel treatments.Signal transducer and activator of transcription 3 (STAT3) is a transcription factor that exerts important biological functions related to cell proliferation, differentiation, survival, angiogenesis and immune response.Aims:In this study, we used RNAscope technology in order to evaluate STAT3 RNA expression in human DLBCL in selected groups of activated B‐cell–like DLBCL (ABC‐DLBCL) patients and germinal center B‐cell–like DLBCL (GBC‐DLBCL) patients.MethodsThis retrospective study reviewed data from patients diagnosed with DLBCL collected from the archive of the Section of Pathology of the University of Bari, Hospital Policlinico, Bari, Italy, between 2009 and 2013. All patients had pathologically confirmed DLBCL. Tumors were divided into two histological subgroups: one that includes ABC patients and another that includes GCB patients.Results:The results have shown that ABC DLBCL tissue samples contained more STAT3‐positive cells as compared with GCB tissue samples. Moreover, the immunofluorescence analysis showed that tumor vessels in ABC samples appeared lined by endothelial cells expressing both FVIII and STAT3 signals, while in GCB samples, only few vessels expressed both FVIII and STAT3. These data confirm other reports showing that STAT3 is highly expressed and activated in ABC‐DLBCL samples and the tumor vessels appeared lined by endothelial cells expressing both FVIII and STAT3.Summary/Conclusion:An improved understanding of tumor biology and the role of the tumor microenvironment has led to advances in the diagnosis, classification, prognostics, as well as novel treatments of patients with hematologic malignancies. Ongoing dynamic and correlation studies of tumor biology and the contribution of the tumor microenvironment should be promoted in the context of novel drug development in order to identify optimal therapies for various lymphomas, and improve the curability of these diseases.
PB2066 DAMAGE OF THE TP53 GENE POLYADENYLATION SIGNAL IN DLBCL
Authors: Voropaeva E. , Pospelova T. , Voevoda M. , Maximov V. , Kolesnikova M. 
Published: June 2019

Background:Single nucleotide replacement rs78378222 lead to change of the normal TP53 gene polyadenylation signal and cause disorders of processing of 3′‐end of mRNA. The experimental data have suggested that rare allele C of the rs78378222 leads to much lower level of TP53 expression and decrease the apoptosis induction level in cells under the influence of genotoxic agents in comparison with allele A. Thus, rs78378222 is thought to be the unique allele variant of TP53 gene with the reduced p53 function.Aims:The purpose of the present study was to reveal the frequency of the rs78378222 of TP53 in tumor tissue of the patients with diffuse large B‐cell lymphoma (DLBCL).MethodsGenomic DNA was isolated from formalin‐embedded paraffin blocks of lymph nodes and extranodal tumor lesions biopsies of 92 patients with DLBCL by phenol‐chloroform extraction method using guanidine. The tissue sections containing at least 70‐80% of the tumor cells were taken. Genotyping of the rs78378222 was performed by the PCR‐RFLP method. To confirm the presence of rare allele C of the rs78378222 Sanger's direct sequencing was performed.Results:The frequency of the rs78378222 in DLBCL tumor tissue was 9/92 (9.8%). It was noted that 5 of 9 patients had minor allele C in the homozygous state. Since the detection of cases of the rare homozygous genotype C/C of the rs78378222 in normal tissue has not described previously, the received results indicated a loss of heterozygosity in the TP53 gene in DLBCL tumor tissue of rs78378222 carriers. Only two of these cases were combined with mutations in the gene. In three cases ‐ loss of heterozygosity was the only aberration in TP53.Summary/Conclusion:Aberrations in 3′‐UTR of TP53 gene may be a universal carcinogenesis mechanism, playing role in pathogenesis of DLBCL as well. The loss of normal allele A rs78378222 stimulates the significant growth of cells malignant potential.
PB2076 MOLECULAR CYTOGENETIC ANALYSIS OF CHROMOSOME 7 ABERRATIONS IN MYELOID DISEASES
Authors: Ransdorfova S. , Onderkova M. , Brezinova J. , Sarova I. , Valerianova M. , Svobodova K. , Lhotska H. , Pavlistova L. , Lizcova L. , Michalova K. , Zemanova Z. , Jonasova A. , Stopka T. , Cermak J. 
Published: June 2019

Background:Complete or partial loss of chromosome 7 is a recurrent chromosomal aberration frequently found in myeloid disorders such as myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). This finding is an important prognostic indicator associated with poor response to the treatment and faster disease progression.Aims:(1) to determine the breakpoints on the long and short arm of chromosome 7 using FISH with locus‐specific probes; (2) to specify the frequency of these rearrangement; and (3) to evaluate overall survival [OS] of patients with chromosome 7 alteration.Methods:Diagnostic bone‐marrow samples of 46 adults with AML/MDS and with chromosome 7 aberration were examined. Karyotypes were analyzed using conventional and molecular cytogenetic techniques: FISH (Abbott, Kreatech), mFISH/mBAND (MetaSystems) and array CGH/SNP (Illumina, Agilent).Results:Monosomy 7 as a sole chromosomal abnormality was found in seven patients. Isolated deletion 7q was proved in one case only. In 38 cases, chromosome 7 aberrations were detected in combination with other changes (n = 16) or as a part of complex karyotype (n = 22). The most frequently altered regions on the long arm were identified in bands 7q22, 7q31 and 7q33‐7q36. In 17 patients (37%), deletion of the EZH2 tumor suppressor gene located in band 7q36.1 was found. The most frequently altered regions on the short arm were identified in bands 7p11‐p12. Rearrangements of 7p were always associated with other chromosomal alterations, the isolated 7p aberration was not proved. In 14 patients (30%), structural aberrations of both short and long arm 7p/7q were detected. In two cases, acquisition of additional clonal aberrations was observed during the disease. OS of the whole group was 6.45 months.Summary/Conclusion:In our cohort, the rearrangement of 7p as a sole aberration was not observed. A large heterogeneity of breakpoints was confirmed on the long arm 7q. High instability of chromosome 7 together with other chromosomal changes represent a marker of poor prognosis and is associated with rapid disease progression. In patients with myeloid malignancies, early detection of 7p/7q aberrations is important to precise prognostication and treatment decision.Supported by MH CZ‐DRO (IHBT, 00023736), RVO‐VFN64165, GACR P302/12/G157, Progres Q28/LF1.
PB2079 INCIDENCE OF MBL AT MDS DIAGNOSIS AND CLINICAL IMPLICATIONS
Authors: Pardalis V. , Dimou M. , Iliakis T. , Bitsani A. , Papaioannou P. , Koudouna A. , Kalyva S. , Markopoulos A. , Grafakos I. , Kyrtsonis M.‐C. , Panagiotidis P. 
Published: June 2019

Background:Monoclonal B lymphocytosis (MBL) which is characterized as asymptomatic monoclonal expansion of< 5 × 109/L circulating B cells has been reported in 5.5% of adults > 65 years of age in the general population (Ghia P et al. Blood 2004). MBL can have the immunophenotype of CLL (CD5+, CD23 + )‐CLL like, however less than 20% have non‐CLL phenotype (CD5‐, CD23‐). They rarely progress to CLL or lymphoma and do not require treatment (Xocheli A et al. Blood 2014). Coexistence of MBL and myelodysplastic syndromes (MDS) is a known phenomenon (Cauwalier et al. Leuk Lymphoma 2002); however the true incidence and the clinical impact of this phenomenon is poorly studied.Aims:We analysed the incidence of concurrent diagnosis of MBL and MDS in the whole cohort of newly diagnosed MDS patients (pts), who referred at our centre between 1/2014 and 1/2019.MethodsWe retrospectively collected data from all the newly diagnosed MDS pts in our centre between 1/1/2014 and 2/1/2019. In particular, we focused on patients’ demographics (gender, age), MDS risk category, incidence of random detection of an MBL population either with immunophenotype or immunohistochemistry in bone marrow and/or peripheral blood and treatment data.Results:Eighty one pts were referred and diagnosed with MDS in our centre during the aforementioned period: 23 female and 52 male with median age of 76 years (range 44‐92), 23 (28.4%) low, 33 (48,1%) Intermediate‐1, 17 (21%) Intermediate‐2 and 2 (2.5%) high IPSS risk score. In 7 (8.6%) pts concurrent MDS and MBL was found. In 4 (4.9%) pts an MBL CLL‐like population and in 3 (3.7%) a non‐CLL‐like population was detected. Six of these 7 pts were treated for MDS: 4 with azacytidine, one with lenalidomide (del 5q MDS) and 1 with danazol. One of those pts was diagnosed with Diffuse Large B cell Lymphoma of peritoneal cavity 15 months after the initiation of azacytidine for MDS and he died after 2 cycles of R‐CHOP regimen. MDS status at the time of death was partial response. No evolution of MBL was presented in the other 6 patients.Summary/Conclusion:Coincidence of MBL in MDS patients in our centre was 8.6%, which is higher than the reported rate for the general population of the same age (5.5%). Despite the fact that in only one patient with MDS, MBL transformed to DLBCL, question between the relationship of MDS and MBL still remains. Issues like impact of MDS therapy to the possibility of transformation of MBL to aggressive B cell lymphomas and relationship between age and coexistence of MBL in MDS patients have to be further elucidated.
PB2082 PHENOTYPIC ALTERATIONS IN CHRONIC MYELOMONOCYTIC LEUKEMIA: SIMILARITIES AND DIFFERENCES MYELODYSPLASTIC SYNDROMES
Authors: Marques J. R. , Pereira‐Cunha F. G. , Duarte G. B. O. , Lorand‐Metze I. 
Published: June 2019

Background:Recently, 3 types of monocytes have been characterized by immunophenotyping in chronic myelomonocytic leukemia (CMML). Increase in monocytes in bone marrow (BM) may also be found in myelodysplastic syndromes (MDS). In a previous study using multiparameter flow cytometry to study BM myeloid precursors in MDS we found monocyte increase in 21% of the patients, with similar frequencies in RAEB and low risk MDS, but this was associated with a worse overall survival.Aims:to study the phenotypic characteristics of BM myelomonocytic precursors and CD34+ progenitors in CMML and compare them with findings observed in MDS.Methodswe studied cases of CMML and low risk MDS entering our Laboratory in 2018. Bone marrow (BM) was collected at the diagnostic work‐up. Diagnosis and classification of the cases was made by WHO 2016 criteria based on peripheral blood counts, BM morphology and cytogenetics. Cases of transitory peripheral cytopenias presenting normal results were used as controls. Immunophenotyping of BM cells was made with and 8‐color platform according to Euroflow. A minimum 300000 cells were acquired.Results:We examined 24 cases of MDS (age 27‐88 years), and 19 CMMLs (age 55‐90 years) which were compared with 12 controls (age 25‐79 years). Concerning immunophenotypic features, SSC of granulocytic precursors/SSC of lymphocytes <6 was seen in 10 MDS and in 9 LMMC cases. Abnormal antigen expression in granulocytic precursors was observed in 11/23 MDS (0 alterations = 12, 1 = 5 and 2 = 6) and 11/17 CMML (0 alterations = 7, 1 = 7 and 2 = 5). Total monocytes were increased in all CMMLs but also in 15 cases of MDS, with similar proportions of classical monocytes (median 94.7% and 93.1% respectively), but increase in monocytes in CMML was mostly dependent on increase in classical monocytes, while in MDS both classical and non‐classical forms increased. Myeloid CD34+ progenitors were >2% in 4 cases of CMML but in none of the MDS cases. Total lymphocytes increased in MDS, but decreased in CMML. In these later, the decrease was more prominent in mature B‐cells, but occurred also in T CD4+ cells.Summary/Conclusion:Immunophenotyping of BM in CMML discloses several abnormalities of monocytic but also of granulocytic precursors, similar to MDS. Besides, decrease in lymphoid cells is similar to what has been described in juvenile myelomonocytic leukemia but different from those seen in low risk MDS.