bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protests, Rallies, Marches, and Social Movements as Organizational Change Agents
Authors: Germain Marie-Line , Robertson Phyllis , Minnis Sarah 
Published: February 2019

The Problem Social movements can impact organizations and employees through changes in policies and procedures in the workplace. Measuring the degree to which collective protests, rallies, and marches have influenced organizational actions can be complex due to the variance in activist practices and the resistance or amiableness of organizations to change. The Solution Protests, rallies, and marches can create a disruptive stage for challenging and confronting organizational policies and practices that maintain power structures. Through the examination of the literature and the review of recent worldwide events, this article illustrates how social movements have affected organizations and led to organizational changes, illuminating change processes in established fields such as business. Specifically, we aim to answer how protests, rallies, and marches influence organizational policies and practices attributed to human resource development (HRD). We conclude by discussing how a recent social movement is applicable to the field of HRD and suggest areas for future research. The Stakeholders This article may be of interest to employees at all levels of the organization, researchers, and practitioners in the field of HRD.
Ligature-induced periodontitis induces systemic inflammation but does not alter acute outcome after stroke in mice
Authors: O'Boyle Conor , Haley Michael J , Lemarchand Eloise , Smith Craig J , Allan Stuart M , Konkel Joanne E , Lawrence Catherine B 
Published: February 2019

Background Stroke is a major cause of disability and mortality. Poorer outcome after stroke is associated with concomitant inflammatory and infectious disease. Periodontitis is a chronic inflammatory disease of the dental supporting structures and is a prominent risk factor for many systemic disorders, including cardiovascular disease and stroke. While epidemiological studies suggest that periodontitis increases the likelihood of stroke, its impact on stroke severity is poorly understood. Here, we sought to determine the contribution of periodontitis to acute stroke pathology. Methods We characterized a murine ligature model of periodontitis for inflammatory responses that could potentially impact stroke outcome. We applied this model and then subjected mice to either transient or permanent middle cerebral artery occlusion. We also enhanced the periodontitis model with repeated intravenous administration of a periodontal-specific lipopolysaccharide to better mimic the clinical condition. Results Ligature-induced periodontitis caused bone loss, bacterial growth, and increased local inflammatory cell trafficking. Systemically, periodontitis increased circulating levels of pro-inflammatory cytokines, and primed bone marrow monocytes to produce elevated tumour necrosis factor-alpha (TNFα). Despite these changes, periodontitis alone or in tandem with repeated lipopolysaccharide challenge did not alter infarct volume, blood–brain barrier breakdown, or systemic inflammation after experimental stroke. Conclusions Our data show that despite elevated systemic inflammation in periodontitis, oral inflammatory disease does not impact acute stroke pathology in terms of severity, determined primarily by infarct volume. This indicates that, at least in this experimental paradigm, periodontitis alone does not alter acute outcome after cerebral ischemia.
Metastatic renal cell carcinoma to the eyelid: an unusual presentation
Authors: Ramez Ahmed M , Hamed Manar , Saad Eman A 
Published: February 2019

Renal cell carcinoma has an unpredictable course with a propensity for metastatic spread. Here we present the case of a female patient with an unusual presentation of metastatic renal cell carcinoma to the eyelid which is extremely rare. Treatment of metastatic renal cell carcinoma is still controversial; however, several authors consider metastasectomy to be a valid option. Unfortunately, little information is available on the utility of systemic treatment after the complete resection of metastatic lesions. Level of evidence: Not applicable for this multicentre audit.
No Benefits of Combining Proximal Row Carpectomy With PIN Neurectomy for Wrist Disorders—A Comparative Study With Systematic Review of the Literature
Authors: Tahta Mesut , Aydin Yener , Erpala Firat , Yildiz Mehmet , Gunal Izge , Sener Muhittin 
Published: February 2019

Background: Posterior interosseous nerve (PIN) resection in combination with proximal row carpectomy (PRC), is a preferred method in order to obtain rapid recovery. However, the contribution of such combination to results isn’t known well. Objectives: We performed a comparative study to evaluate the effects of PIN neurectomy for PRC and a systematic review of the literature was performed to identify whether such combination has an advantage. Methods: Patients with wrist diseases who underwent PRC were evaluated retrospectively. Patients without PIN neurectomy (group 1, n = 7) and with PIN neurectomy (group 2, n = 8) were compared in respect of mean age, follow-up, gender, Q-DASH, VAS, MAYO wrist scores, flexion–extension/radial–ulnar deviation range of motion at final follow-up. The MEDLINE database was searched for studies published between 2005 and 2015, as the second part of the study. The following keywords were used: “proximal,” “row,” “carpectomy.” Studies, which met the inclusion criteria, were evaluated in terms of such combination. Results: There were no significant difference between the groups in regard with age ( P = .463), follow-up period ( P = .728), the ranges of flexion–extension ( P = .431) and radio–ulnar deviation ( P = .689), Q-DASH ( P = .452), and MAYO scores ( P = .728). In the second part of the study, 12 studies met the inclusion criteria and none of them was specifically evaluating such combination. Only one study had specific comments on PRC with PIN neurectomy. Conclusion: According to our study (which, to our knowledge, was the first comparative study in the literature), we advocate not to combine PRC with PIN neurectomy for such an approach has no advantage.
Assessment of Clinical Outcomes and Patient Response to Gingival Depigmentation by Scalpel Surgical Stripping and Diode Laser: A Randomized Split-Mouth Study
Authors: Chhina Shivjot , Gakhar Abhishek , Gupta Stuti , ss Shradha , Sharma Ejya , Arora Sachit Anand 
Published: February 2019

Oral melanin pigmentation is a ubiquitous presentation in the oral cavity. It could be a cause of psychological distress to many subjects due to aesthetic reasons. Aims and Objectives: This study attempts to compare the laser and scalpel depigmentation techniques. It also includes a comparison related to the Visual Analog Scale, patient comfort, healing response and time taken for the procedure and recurrence of pigmentation. Materials and Methods: A total of 10 patients were included in the study. A split-mouth approach comparing the scalpel technique with that of the diode laser technique was planned. Results and Conclusion: The results of the present study revealed that both laser and scalpel techniques are equally effective for depigmentation, yielding aesthetic results.
Epigenetic control of early dendritic cell lineage specification by the transcription factor IRF8 in mice
Authors: Kurotaki Daisuke , Kawase Wataru , Sasaki Haruka , Nakabayashi Jun , Nishiyama Akira , Morse Herbert C. , Ozato Keiko , Suzuki Yutaka , Tamura Tomohiko 
Published: February 2019

Abstract
Dendritic cells (DCs), which are vital for immune responses, are derived from bone marrow hematopoietic stem cells via common DC progenitors (CDPs). DC lineage fate decisions occurring at stages much earlier than CDPs have recently been recognized, yet the mechanism remains elusive. By single-cell RNA-sequencing, in vivo cell transfer experiments, and an assay for transposase-accessible chromatin sequencing using wild-type, IRF8-GFP chimera knock-in or IRF8-knockout mice, we demonstrate that IRF8 regulates chromatin at the lymphoid-primed multipotent progenitor (LMPP) stage to induce early commitment toward DCs. A low but significant expression of IRF8, a transcription factor essential for DC and monocyte development, was initiated in a subpopulation within LMPPs. These IRF8+ LMPPs were derived from IRF8– LMPPs and predominantly produced DCs, especially classical DC1s, potentially via known progenitors, such as monocyte-DC progenitors, CDPs, and preclassical DCs. IRF8+ LMPPs did not generate significant numbers of monocytes, neutrophils, or lymphocytes. Although IRF8– and IRF8+ LMPPs displayed very similar global gene expression patterns, the chromatin of enhancers near DC lineage genes was more accessible in IRF8+ LMPPs than in IRF8– LMPPs, an epigenetic change dependent on IRF8. The majority of the genes epigenetically primed by IRF8 were still transcriptionally inactive at the LMPP stage, but were highly expressed in the downstream DC lineage populations such as CDPs. Therefore, early expression of the key transcription factor IRF8 changes chromatin states in otherwise multipotent progenitors, biasing their fate decision toward DCs.
Characteristics of patients with myeloproliferative neoplasms with lymphoma, with or without JAK inhibitor therapy
Authors: Pemmaraju Naveen , Kantarjian Hagop , Nastoupil Loretta , Dupuis Megan , Zhou Lisa , Pierce Sherry , Patel Keyur P. , Masarova Lucia , Cortes Jorge , Verstovsek Srdan 
Published: February 2019

Abstract
There is a Blood Commentary on this article in this issue.
How mRNA therapeutics are entering the monoclonal antibody field
Authors: Van Hoecke Lien , Roose Kenny 
Published: February 2019

AbstractIn 1975, Milstein and Köhler revolutionized the medical world with the development of the hybridoma technique to produce monoclonal antibodies. Since then, monoclonal antibodies have entered almost every branch of biomedical research. Antibodies are now used as frontline therapeutics in highly divergent indications, ranging from autoimmune disease over allergic asthma to cancer. Wider accessibility and implementation of antibody-based therapeutics is however hindered by manufacturing challenges and high development costs inherent to protein-based drugs. For these reasons, alternative ways are being pursued to produce and deliver antibodies more cost-effectively without hampering safety. Over the past decade, messenger RNA (mRNA) based drugs have emerged as a highly appealing new class of biologics that can be used to encode any protein of interest directly in vivo. Whereas current clinical efforts to use mRNA as a drug are mainly situated at the level of prophylactic and therapeutic vaccination, three recent preclinical studies have addressed the feasibility of using mRNA to encode therapeutic antibodies directly in vivo. Here, we highlight the potential of mRNA-based approaches to solve several of the issues associated with antibodies produced and delivered in protein format. Nonetheless, we also identify key hurdles that mRNA-based approaches still need to take to fulfill this potential and ultimately replace the current protein antibody format.
An approach for accelerated isolation of genetically manipulated cell clones with reduced clonal variability
Authors: Casden Natania , Behar Oded 
Published: February 2019

ABSTRACT
Genomic editing methods, such as the CRISPR/Cas9 system, are routinely used to study gene function in somatic cells. Owing to the heterogeneity of mutations, it is necessary to purify cell clones grown from high dilution to the point of colony formation, which can be a time-consuming process. Here, we tested a modified approach in which we seeded cells at high dilution, together with non-edited carrier cells. As a comparison, cells were also grown at high dilution with conditioned medium from a high-density culture. When using carrier cells or conditioned medium, the formation of cell colonies is accelerated. Additionally, clones grown with carrier cells are more similar to the parental lines in terms of their tumorigenic properties. Surprisingly, key signaling cascades are highly divergent between clones isolated from low-density cultures, even with conditioned medium, in contrast to clones isolated with carrier cells. Thus, our study uncovers a significant limitation using the common approach of isolating cell clones following genetic modifications and suggests an alternative method that mitigates the problem of heterogeneity of gene expression between clones.
This article has an associated First Person interview with the first author of the paper.
PML is recruited to heterochromatin during S phase and represses DAXX-mediated histone H3.3 chromatin assembly
Authors: Shastrula Prashanth Krishna , Sierra Isabel , Deng Zhong , Keeney Frederick , Hayden James E. , Lieberman Paul M. , Janicki Susan M. 
Published: February 2019

ABSTRACT
The incorporation of the histone H3 variant, H3.3, into chromatin by the H3.3-specific chaperone DAXX and the ATP-dependent chromatin remodeling factor ATRX is a critical mechanism for silencing repetitive DNA. DAXX and ATRX are also components of promyelocytic nuclear bodies (PML-NBs), which have been identified as sites of H3.3 chromatin assembly. Here, we use a transgene array that can be visualized in single living cells to investigate the mechanisms that recruit PML-NB proteins (i.e. PML, DAXX, ATRX, and SUMO-1, SUMO-2 and SUMO-3) to heterochromatin and their functions in H3.3 chromatin assembly. We show that DAXX and PML are recruited to the array through distinct SUMOylation-dependent mechanisms. Additionally, PML is recruited during S phase and its depletion increases H3.3 deposition. Since this effect is abrogated when PML and DAXX are co-depleted, it is likely that PML represses DAXX-mediated H3.3 chromatin assembly. Taken together, these results suggest that, at heterochromatin, PML-NBs coordinate H3.3 chromatin assembly with DNA replication, which has important implications for understanding how transcriptional silencing is established and maintained.
Measurability and reliability of assessments of root length and marginal bone level in cone beam CT and intraoral radiography: a study of adolescents
Authors: Al-Okshi Ayman , Paulsson Liselotte , Rohlin Madeleine , Ebrahim Eman , Lindh Christina 
Published: February 2019

Objectives: To evaluate measurability and reliability of measurements of root length and marginal bone level in CBCT, periapical (PA) and bitewing (BW) radiographs. Methods: CBCT of both jaws, PA of maxillary incisors and posterior BW radiographs of 10 adolescents (mean age 13.4) were selected. The radiographs comprised part of the baseline examinations of a trial of orthodontic treatment. Six raters assessed measurability and measured root length and marginal bone level. Three raters repeated their assessments. Measurability was expressed as frequency of interpretable sites and reliability as intraclass correlation coefficient (ICC). Results: Measurability was 100 % in CBCT and 95 % in PA of maxillary incisors for root length measurements. For marginal bone level, measurability was 100 % in CBCT, 76 % in PA and 86 % in posterior BW. Mean ICC for interrater reliability for root length measurements in CBCT was 0.88 (range 0.27–0.96 among different teeth) and 0.69 in PA of maxillary incisors. For marginal bone level measurements, mean ICC was 0.4 in CBCT, 0.38 in PA of maxillary incisors and 0.4 in posterior BW. Intrarater reliability varied among methods, root length or marginal bone level and among raters, except for root length measurements in CBCT, which presented high reliability (above 0.8) for all raters. Conclusions: As measurability and reliability were high for root length measurements in CBCT, this may be the method of choice for scientific analyses in orthodontics. For clinical praxis, we recommend PA following the “as low as diagnostically acceptable” principle, as clinical decisions seem to be influenced only when severe root resorption occurs.
Pemanfaatan E-Journal oleh Mahasiswa pada Perpustakaan Perguruan Tinggi di Jakarta
Authors: Wicaksono Hendra 
Published: February 2019

Penelitian ini menganalisis pemanfaatan ejournal oleh mahasiswa pada perguruan tinggi di Jakarta. Penelitian ini menggunakan pendekatan kuantitatif dengan menggunakan metode diskriptif. Pengumpulan data dilakukan dengan penyebaran kuesioner, observasi, dan wawancara. Penelitian dilakukan dengan cara penyebaran kuesioner pada 122 responden berisi 19 butir pertanyaan pada mahasiswa dan pustakawan disertai dengan wawancara. Proses terakhir peneliti melakukan analisis data yang sudah di olah menggunakan software Microsoft Excel 2013. Data yang telah diolah, dianalisis menggunakan skala likert berdasarkan teori SWOT strength (Kekuatan), Weakness (Kelemahan), Oppurtinities (Peluang), dan Threats (ancaman) . Hasil penelitian menunjukkan bahwa mayoritas responden menjawab positif tentang fasilitas E-Journal yang dilanggan namun sebagian kecil responden menjawab sering kesulitan dalam memanfaatkan E-Journal . Kesulitan ini terjadi karena tidak mengetahui bahwa E-Journal yang disediakan oleh perpustakaan dapat diakses dari rumah. Maka dapat dilihat bahwa mahasiswa sangat antusias terhadap fasilitas E-Journal yang dilanggan. Kemudian dari pertanyaan tentang layanan ejournal di perpustakaan, dapat dilihat bahwa mahasiswa menilai layanan E-Journal yang dilanggan cukup baik.
Kolaborasi dan Produktivitas Penulis pada Prosiding Lokakarya Nasional Dokumentasi dan Informasi PDII LIPI Periode 2013-2017
Authors: Rahayu Rochani Nani , Tupan Tupan 
Published: February 2019

Prosiding Lokakarya Nasional Dokumentasi dan Informasi periode 2013 – 2017 dikaji dengan metode bibliometrik untuk mengetahui ; 1) Jumlah artikel; 2) Pola kepengarangan; 3) Derajat kolaborasi; 4) Produktivitas penulis. Hasil penelitian menunjukkan bahwa : 1) jumlah artikel sebanyak 66 judul dengan jumlah penulis 101 orang; 2) Pola kepengarangan yang diteliti menunjukkan bahwa artikel terbanyak ditulis oleh penulis tunggal yaitu sebanyak 42 artikel (63,64%), disusul oleh dua penulis sebanyak 14 artikel (21,21%), tiga penulis sebanyak 6 artikel (9,09%), empat penulis 3 artikel (4,55% ), tidak dijumpai penulis yang berkolaborasi secara berlima, dan 6 penulis 1 artikel (1,51%); 3) Nilai derajat kolaborasi dijumpai sebesar 0,39 dan 4) Dijumpai 10 orang penulis paling produktif menulis 2 judul artikel dengan jumlah yaitu Ambar Yoganingrum, Dwi Ridho Aulia, Heri Abi Burachman Hakim, Himawanto, Madiareni Sulaiman, Putu Laxman Pendit, Rulina Rachmawati, Sadjuga, Tupan dan Wahid Nashihuddin, dan rata – rata penulis/ artkel adalah 1,53. Kesimpulan penelitian adalah bahwa kolaborasi penulis dalam Prosiding Lokakarya Dokumentasi dan Informasi periode 2013 – 2017 masih rendah, karena artikel masih didominasi oleh penulis tunggal. Hal tersebut diperkuat dengan nilai derajat kolaborasi yang rendah, dan produktivitas penulis juga rendah karena tidak ada penulis yang menulis lebih dari 2 artikel.
Coronin 1A deficiency identified by newborn screening for severe combined immunodeficiency
Authors: Dinur Schejter Yael , Mandola Amarilla , Merico Daniele , Reid Brenda 
Published: February 2019

Introduction: Coronin 1A belongs to a large family of actin regulatory proteins with a role in T cell homeostasis. A role for coronin 1A was also observed in macrophages, NK, and neuronal cells. To date, coronin 1A deficiency has been described in relatively few patients with combined immunodeficiency. Aim: We studied here the molecular and genetic basis of immunodeficiency detected by newborn screening for severe combined immunodeficiency. Methods: Patient data was collected in accordance with REB approved protocols. Immune work up, including T and B cell proliferative responses and serum concentrations of immunoglobulins, was performed. Next generation sequencing techniques and cellular analyses were also utilized. Results: The patient presented with T cell lymphopenia, reduction in CD4+CD45Ra+ cells and hypogammaglobulinemia. Uniquely, she also had persistent severe neutropenia. Whole exome sequencing and Sanger confirmation revealed a novel homozygous mutation in coronin 1A. Conclusion: Coronin 1A deficiency can be detected after birth by T cell receptor excision circle-based newborn screening. Statement of novelty: We report here a patient with a novel mutation in coronin 1A, identified during newborn screening with low T cell receptor excision circle levels and neutropenia, which is a unique finding in this condition.
Cross-industry growth differences with asymmetric industries and endogenous market structure
Authors: Huang Chien-Yu , Ji Lei 
Published: February 2019

Abstract
We develop a two-industry R&D growth model with a free-entry endogenous market structure to evaluate the impact of industrial fundamentals on cross-industry differences of TFP growth and R&D intensity. Endogenous market structure in our model allows the firm’s market size to respond to the firm’s entry and exit which complements the models with an exogenous market structure in the previous literature. We find that surprisingly, an industry with a relatively high R&D productivity or appropriability exhibits “relatively” low in-house innovation growth and R&D intensity during transition. Moreoever, we examine the effects of R&D subsidies and patent breadth policies on industry differences by implementing both asymmetric and symmetric policy rules. We find that only asymmetric R&D subsidies have impacts on TFP growth and R&D intensity differences.
A global random walk on spheres algorithm for transient heat equation and some extensions
Authors: Sabelfeld Karl K. 
Published: February 2019

Abstract
We suggest in this paper a global Random Walk on Spheres (gRWS) method for solving transient boundary value problems, which, in contrast to the classical RWS method, calculates the solution in any desired family of m prescribed points.
The method uses only N trajectories in contrast to mN trajectories in the conventional RWS algorithm.
The idea is based on the symmetry property of the Green function and a double randomization approach.
We present the gRWS method for the heat equation with arbitrary initial and boundary conditions, and the Laplace equation.
Detailed description is given for 3D problems; the 2D problems can be treated analogously.
Further extensions to advection-diffusion-reaction equations will be presented in a forthcoming paper.
The persistence of expression: Clusivity, partial co-reference, and socioeconomic differentiation of first person plural subject pronoun expression in Spanish
Authors: LaCasse Dora 
Published: February 2019

AbstractThis study revisits variable subject pronoun expression in Spanish, bringing to bear insights from cross-linguistic patterns of person-number systems. Based on 2259 tokens from two corpora of Mexican Spanish representing distinct social classes, the study focuses solely on first person plural (1pl) subject pronouns, revealing unique aspects of variablenosotrosexpression. Evidence is offered in favor of a more nuanced measure of switch reference for non-singular grammatical persons through an analysis of the local effects of partial co-referentiality. This measure reconciles the large body of work on switch reference with Cameron’s (1995) measures of reference chains. Additionally, topic persistence — heretofore neglected in prior studies — conditions the variation, with subsequent mentions in the thematic paragraph favoring expressed pronouns. An investigation of clusivity demonstrates that Spanish 1pl subject pronoun expression is sensitive to a distinction grammaticalized in other languages, though subject pronoun rates across clusivities differ from previous results from Peninsular Spanish (Posio 2012). Finally, while subject pronoun expression is generally not sensitive to social factors, distributions of 1pl subjects according to clusivity differ between corpora. Results reveal the style and topic-conditioned differences in contextual distributions that underlie apparent social class differences in subject expression constraints.