bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of HPLC Analytical Method for Chlorantraniliprole in Bulk and in the Formulation
Authors: Kapupara Pankaj P. , Sorathiya Vandana , Patel Ravi P. , Shah Ketan V. 
Published: April 2018

Abstract
Background: Chlorantraniliprole is widely used as a pesticide. It is only soluble in dimethyl formamide. However, most of the reported methods used acetonitrile and other solvents.
Aim: To develop rapid, accurate, precise, and sensitive HPLC method for chlorantraniliprole.
Materials and methods: 10 μG/mL of chlorantraniliprole containing solution was injected into the HPLC system and run in different solvent systems. 10 μG/mL solutions of chlorantraniprole was injected in a column with 20 μL microsyringe. The chromatogram was run for appropriate minutes with mobile phase water. The flow rate was set to 1 mL/min and detection was carried out at wavelength 270 nm. The method is validated by measuring the limit of quantitation, limit of detection, repeatability, intraday precision, inter-day precision, and accuracy. Analysis of marketed formulation, bringle, and tomato for chlorantraniprole content was also made for the developed analytical method.
Results: There was linearity of chlorantraniliprole for calibration curve in between 1 and 5 μG/mL concentration. The perfect sharp peak observed in water at a retention time of 6.28 min. The limit of detection and the limit of quantitation were 0.0050 μG/mL and 0.0152 μG/mL, respectively. The study reported 99.55% repeatability, 99.49% intraday precision, 99.65% interday precision, and 99.27% accuracy.
Conclusion: The rapid, accurate, precise, and sensitive HPLC method for the detection of chlorantraniliprole using dimethyl formamide was developed.
Antidepressant Effect and Recognition Memory Improvement of Two Novel Plant Extract Combinations - Antistress I and Anti-stress II on Rats Subjected to a Model of Mild Chronic Stress
Authors: Kandilarov Ilin K. , Zlatanova Hristina I. , Georgieva-Kotetarova Maria T. , Kostadinova Ivanka I. , Katsarova Mariana N. , Dimitrova Stela Z. , Lukanov Ludmil K. , Sadakov Ferit 
Published: April 2018

Abstract
Background: Chronic stress is one of the main factors which lead to depression – a psychiatric disorder affecting millions of people and predicted to be the second ranked cause of premature death in 2020. Depression is often associated with cognitive disturbances and memory deficit. Plant based therapy could be effective in the treatment of mild to moderate depression due to its low level of adverse reaction, its good tolerability and compliance.
Materials and methods: 72 male Wistar rats, divided in 9 groups were given orally for 8 weeks two combinations of dry plant extracts – Antistress I and Antistress II and five individual dry extracts obtained from Serratula coronata, Hypericum perforatum, Valeriana officinalis, Crataegus monogyna and Melissa officinalis. The animals were exposed to a chronic unpredictable mild stress for 8 weeks. The depression-like symptoms were evaluated with Forced swim test while the assessment of the memory deficit was performed with Novel object recognition test.
Results: Antistress II demonstrates antidepressant effect while Antistress I doesn’t improve the depressive-like symptoms. The individual extracts of Hypericum perforatum and Valeriana officinalis also possess antidepressant properties. Antistress II improves the cognition as well as the individual extracts of Hypericum perforatum, Valeriana officinalis and especially Serratula coronata. Dry extract from Serratula tend to have the best effect regarding the recognition memory. The effect of Antistress I on memory deficit is negligible.
Conclusions: Antistress II possesses antidepressant effect and improves the recognition memory while Antistress I doesn’t demonstrate any of the above-described effects.
Mobilization and Stimulation of Neuromuscular Tissue (MASONT) for Stroke Survivors
Authors: Athanasiadis Dimitrios , Dionyssiotis Yannis , Papathanasiou Jannis , Stefas Eleftherios 
Published: April 2018

Abstract
Mobilization and Stimulation of Neuromuscular Tissue (MASONT) is a newly invented somatosensory intervention used for the recovery of function in stroke patients. This paper aims to offer a more spherical view on the new technique. To that end, a basic manual of MASONT’s application is provided, along with its rational of use. Moreover, clinical observations on the application of the technique and its effects on neurological patients are presented, as well as, a report on its safety.
Oblong Sesamoid Bone Appearance into the Nuchal Ligament. A Case Report
Authors: Tzatzairis Themistoklis , Papathanasiou Jannis V. , Ververidis Athanasios , Kazakos Ioannis , Drosos Georgios , Tilkeridis Konstantinos 
Published: April 2018

Abstract
Radio-opaque formations behind the spinous processes of the cervical spine are really rare. They were described in 1929 for the first time. Sesamoid ossicles are found close to a bone or a joint and are usually small. Some sesamoids form part of normal human anatomy, while others are normal anatomical variants. We report a case of a 73-year-old Caucasian male who visited the outpatient clinic complaining about chronic cervical pain. An oblong sesamoid accidentally discovered during routine anteroposterior (AP) and lateral x-ray, within the nuchal ligament (NL) at the C3-C6 spinous processes level. A cervical MRI scan was performed and the result showed ‘fabella nuchae’. This type of sesamoids are rarely symptomatic, but it should be differentially diagnosed anyway. We review its morphology, the mechanical factors of the region acting throughout life and last, but not least, the differential diagnosis.
Lignin-based coatings for controlled P-release fertilizer consisting of granulated simple superphosphate
Authors: Rotondo Felipe , Coniglio Rodrigo , Cantera Leandro , Di Pascua Ignacio , Clavijo Leonardo , Dieste Andrés 
Published: April 2018

AbstractA novel lignin-based slow release fertilizer with low environmental impact has been developed. More precisely, a granulated simple superphosphate fertilizer, consisting of calcium phosphate monobasic [Ca(H2PO4)2·H2O] and gypsum (CaSO4·2H2O) was coated with modified kraft lignins and the diffusion of phosphorus was observed as a function of time. The lignin was hydroxymethylated with formaldehyde and subsequently cross-linked with phenol-formaldehyde resin resulting in HML-PF as coating. Moreover, coating films were prepared from a mixture of acetylated lignin (Lac) and acetylated cellulose (Cellac). Both coatings show similar permeability to calcium phosphate and controlled effectively the P-release, particularly at the initial stages of the experiment. The P-release was linear in the decay phase but there is no lag time in the process. A significant P amount was not released from the particles coated with HML-PF, i.e. 80–50% remained irreversible bound, depending on the coating formulation. The maximum fractional P-release varied among the different coatings tested. Phosphorus is partly retained inside the slightly soluble calcium sulfate matrix.
The Impact of European Economic Integration on Migration in the European Union
Authors: Simionescu Mihaela 
Published: April 2018

AbstractThe recent enlargement of the EU (since 2004) and the United Kingdom's decision to leave the European Union have prompted a growing research interest in the political and academic environment because of the causes and consequences of migration between the CEE countries and those in the Western Europe. In this study, the effects of European economic integration on the number of EU-15 immigrants from the newly integrated EU countries were assessed by econometric techniques. According to panel data models, in the period 2000-2015, the number of migrants from the new member states of the EU has increased, in average, with more than 2200 people only due to their EU membership. This result reflects the positive impact of European economic integration on the number of emigrants from the CEE countries that chose the EU-15 states as destination countries. Moreover, according to some ridge Bayesian regressions, during the period 2004-2015, the EU-15 immigrants coming from the EU-13 states did not negatively affect the economic growth of the EU-15 countries.
Principal Management Concepts in Greek Public Sector: Part I – The Common Assessment Framework
Authors: Papalazarou Ioannis , Tsoulfas Giannis T. 
Published: April 2018

AbstractIn the modern era of internationalization, interconnection and rapid technological changes the obligation of the public sector to provide the best possible services to citizens have become challenging tasks. The incorporation of Total Quality Management principles and tools in public services can prove to be decisive given that they contribute to the improvement of the services provided, offer better customer service, help in understanding how the agency is organised and operates, as well as contribute in changing the attitude of employees towards the adoption of a quality culture. Since the mid-00’s, several programmes have been implemented in Greece in order to improve the quality of public services which was, up to that point, dictated only by the need to consolidate relative EU financial resources. This paper is the first part of a study about the application of principal management concepts in Greek public sector since year 2004 when Law 3230/2004 was introduced. In particular it tries to record and assess the experience of the implementation of the “Common Assessment Framework” in Greek public services. Towards this end, the views and opinions of experts from the public sector are analysed with the use of interviews and questionnaires.
Distance Education in Romanian Higher Education
Authors: Ene Andreea Bianca 
Published: April 2018

Abstract
The global diffusion of Internet involves economic, political and demographic factors that can predict in real time. In this article, we demonstrate that according to data provided by EUROSTAT, the number of people looking for a job in Romania it is correlated with specific query terms using Google Trends. Search engine data is used to “predict the present” values of different economic indicators. The obtained results are compared with the classical method of developing the economic indicators, with official EUROSTAT employment data. In this paper, we demonstrate that the new methods to extract the economic indicators from web technologies are accurate.
Tax Policy: What Does SMEs Say?
Authors: Nuswantara Dian Anita , Prastiwi Dewi , Aisyaturahmi  
Published: April 2018

Abstract
Tax is one of the main sources of government revenue in Indonesia. Unfortunately the enormous population has not been able to make tax revenues so great. The cost to issue a new policy package should be accompanied by increased taxpayer compliance, as indicated by tax revenue. But until now the increase in tax revenue is due to the higher the value of money. Based on the above, it needs to be studied and analysed on how the critical analysis of income tax policy for individual taxpayers of small and medium enterprises (SMEs).
A qualitative approach using phenomenological studies is designed to gain an explanation of the above phenomena in terms of the taxpayer's perspective. Survey technique is the main data collection technique used in this study. The final process of the research is expected to find the right formulation of the current policy and find the policy formulation that can be used as input for policy makers in the field of taxation.
The Phenomenon of Climate Change in Organization and HR- Related Literature: A Conceptual Brief Analysis
Authors: Mousa Mohamed 
Published: April 2018

Abstract
Climate change has become one of the main challenges facing humanity. Over the past decade, this phenomenon, which may have been caused by natural variability and/or human activity, has attracted many scholars from different scientific disciplines to warn of its potential consequences. The author of this paper has decided to address the existence of this important phenomenon in organizational literature. However, upon exploring different academic databases, the rarity of research focusing on climate change and its relationship and/or effect on HR or organizational aspects became obvious. Accordingly, the author recommends other HR and organizational scholars devote considerable space to this phenomenon in their field.
Radix Glehniae extract inhibits migration and invasion of lung cancer cells
Authors: Zhen-fei Wang , Li Liu , Lin Liang , Qin Hao 
Published: April 2018

Abstract

Objective
The aim of this study was to investigate the effect of Radix Glehniae on the migration and invasion abilities of lung cancer cells.

Methods
Normal bronchial cell line 16HBE and lung cancer cell line SK-MES-1 were treated with Radix Glehniae extract. Proliferation, migration, and invasion abilities were determined by Cell Counting Kit (CCK)-8, Transwell, and Matrigel assays, respectively. The expression and secretion levels of tissue inhibitor of metalloproteinases 2 were detected by quantitative PCR and enzyme-linked immunosorbent assay, respectively.

Results
Radix Glehniae extract inhibited the migration and invasion abilities of SK-MES-1 cells and enhanced TIMP2 expression and secretion by SK-MES-1 cells, without causing toxicity to 16HBE cells.

Conclusion
Radix Glehniae is useful in lung cancer treatment.
Abnormal platelet count correlates with poor survival in hepatocellular carcinoma
Authors: Gu Lei , Wen Wen , Wu ZhiXian , Bai Kai , Liu Wei , Lai GuoXiang , Li DongLiang 
Published: April 2018

AbstractBackgroundNormal platelet (PLT) plays a vital role in thrombosis, the inflammatory response, and liver regeneration. The effect of abnormal PLT counts has been seldom explored in hepatocellular carcinoma (HCC); hence, this investigation was conducted to evaluate the prognostic importance of preoperative abnormal PLT count in HCC patients after liver resection retrospectively.MethodologyThe PLT counts were determined using Sysmex XT-1800i automated hematology analyzer and its matching reagents. Patients were divided into two groups: a normal PLT group and an abnormal PLT group. Chi-square test, Kaplan–Meier method, and Cox univariable and multivariable regressions were utilized to analyze the data.ResultsA total of 391 HCC patients who underwent liver resection were included in this study. The overall survival (OS) rates were 59% and 31%, and the median survival time was 69 months and 31 months in the normal and abnormal PLT groups, respectively. The PLT level was associated with OS in univariate and multivariate analyses (hazard ratio [HR], 1.991 [95% confidence interval {CI}, 1.412–2.808] and HR, 2.217 [95% CI, 1.556–3.159], respectively).ConclusionsPatients with normal PLT had a better outcome in terms of OS. The results suggested that abnormal PLT count is an independent prognostic factor for HCC patients after liver resection.
Hematotoxicity of intratracheally instilled arsenic trioxide in rats
Authors: Su Mingxing , Sun Congsong , Wang Haiying , Yuan Chunyu , Guo Ruixia , Liang Yajie , Liu Chao , Wang Qiang 
Published: April 2018

AbstractThe study aimed to investigate the correlation between concentration of inhaled arsenic trioxide and dynamic changes in hematotoxicity in rats. Wistar rats were randomly divided into four study groups that were treated with saline (control) or arsenic trioxide at a low (0.1 mg/mL), medium (1 mg/mL), or high (10 mg/mL) dose by intratracheal instillation. Blood samples were collected for analysis at 6, 12, 24, 48, and 72 h after exposure. Compared with the control group, intratracheal instillation of arsenic trioxide affected hematopoietic differentiation in rats, leading to blood cell changes that were related to observation time and concentration.
Similarity of regulatory network between leukemia stem cells and normal hemopoietic stem cells
Authors: Li Zhi-Jian , Sui Xing-Ling , Yang Xue-Bo , Sun Wen 
Published: April 2018

AbstractTo reveal the biology of AML, we compared gene-expression profiles between normal hematopoietic cells from 38 healthy donors and leukemic blasts (LBs) from 26 AML patients. We defined the comparison of LB and unselected BM as experiment 1, LB and CD34+ isolated from BM as experiment 2, LB and unselected PB as experiment 3, and LB and CD34+ isolated from PB as experiment 4. Then, protein–protein interaction network of DEGs was constructed to identify critical genes. Regulatory impact factors were used to identify critical transcription factors from the differential co-expression network constructed via reanalyzing the microarray profile from the perspective of differential co-expression. Gene ontology enrichment was performed to extract biological meaning. The comparison among the number of DEGs obtained in four experiments showed that cells did not tend to differentiation and CD34+ was more similar to cancer stem cells. Based on the results of protein–protein interaction network,CREBBP,F2RL1,MCM2, andTP53were respectively the key genes in experiments 1, 2, 3, and 4. From gene ontology analysis, we found that immune response was the most common one in four stages. Our results might provide a platform for determining the pathology and therapy of AML.
Attention Selectively Gates Afferent Signal Transmission to Area V4
Authors: Grothe Iris , Rotermund David , Neitzel Simon David , Mandon Sunita , Ernst Udo Alexander , Kreiter Andreas K. , Pawelzik Klaus Richard 
Published: April 2018

Selective attention allows focusing on only part of the incoming sensory information. Neurons in the extrastriate visual cortex reflect such selective processing when different stimuli are simultaneously present in their large receptive fields. Their spiking response then resembles the response to the attended stimulus when presented in isolation. Unclear is where in the neuronal pathway attention intervenes to achieve such selective signal routing and processing. To investigate this question, we tagged two equivalent visual stimuli by independent broadband luminance noise and used the spectral coherence of these behaviorally irrelevant signals with the field potential of a local neuronal population in male macaque monkeys' area V4 as a measure for their respective causal influences. This new experimental paradigm revealed that signal transmission was considerably weaker for the not-attended stimulus. Furthermore, our results show that attention does not need to modulate responses in the input populations sending signals to V4 to selectively represent a stimulus, nor do they suggest a change of the V4 neurons' output gain depending on their feature similarity with the stimuli. Our results rather imply that selective attention uses a gating mechanism comprising the synaptic “inputs” that transmit signals from upstream areas into the V4 neurons. A minimal model implementing attention-dependent routing by gamma-band synchrony replicated the attentional gating effect and the signals' spectral transfer characteristics. It supports the proposal that selective interareal gamma-band synchrony subserves signal routing and explains our experimental finding that attention selectively gates signals already at the level of afferent synaptic input.SIGNIFICANCE STATEMENTDepending on the behavioral context, the brain needs to channel the flow of information through its networks of massively interconnected neurons. We designed an experiment that allows to causally assess routing of information originating from an attended object. We found that attention “gates” signals at the interplay between afferent fibers and the local neurons. A minimal model demonstrated that coherent gamma-rhythmic activity (∼60 Hz) between local neurons and their afferent-providing input neurons can realize the gating. Importantly, the attended signals did not need to be amplified already in an earlier processing stage, nor did they get amplified by a simple output response modulation. The method provides a useful tool to study mechanisms of dynamic network configuration underlying cognitive processes.
The Role of the Microglial Cx3cr1 Pathway in the Postnatal Maturation of Retinal Photoreceptors
Authors: Jobling Andrew I. , Waugh Michelle , Vessey Kirstan A. , Phipps Joanna A. , Trogrlic Lidia , Greferath Una , Mills Samuel A. , Tan Zhi L. , Ward Michelle M. , Fletcher Erica L. 
Published: April 2018

Microglia are the resident immune cells of the CNS, and their response to infection, injury and disease is well documented. More recently, microglia have been shown to play a role in normal CNS development, with the fractalkine-Cx3cr1 signaling pathway of particular importance. This work describes the interaction between the light-sensitive photoreceptors and microglia during eye opening, a time of postnatal photoreceptor maturation. Genetic removal of Cx3cr1 (Cx3cr1GFP/GFP) led to an early retinal dysfunction soon after eye opening [postnatal day 17 (P17)] and cone photoreceptor loss (P30 onward) in mice of either sex. This dysfunction occurred at a time when fractalkine expression was predominantly outer retinal, when there was an increased microglial presence near the photoreceptor layer and increased microglial–cone photoreceptor contacts. Photoreceptor maturation and outer segment elongation was coincident with increased opsin photopigment expression in wild-type retina, while this was aberrant in theCx3cr1GFP/GFPretina and outer segment length was reduced. A beadchip array highlighted Cx3cr1 regulation of genes involved in the photoreceptor cilium, a key structure that is important for outer segment elongation. This was confirmed with quantitative PCR with specific cilium-related genes,RpgrandRpgrip1, downregulated at eye opening (P14). While the overall cilium structure was unaffected, expression of Rpgr, Rpgrip1, and centrin were restricted to more proximal regions of the transitional zone. This study highlighted a novel role for microglia in postnatal neuronal development within the retina, with loss of fractalkine–Cx3cr1 signaling leading to an altered distribution of cilium proteins, failure of outer segment elongation and ultimately cone photoreceptor loss.SIGNIFICANCE STATEMENTMicroglia are involved in CNS development and disease. This work highlights the role of microglia in postnatal development of the light-detecting photoreceptor neurons within the mouse retina. Loss of the microglial Cx3cr1 signaling pathway resulted in specific alterations in the cilium, a key structure in photoreceptor outer segment elongation. The distribution of key components of the cilium transitional zone, Rpgr, Rpgrip1, and centrin, were altered in retinae lacking Cx3cr1 with reduced outer segment length and cone photoreceptor death observed at later postnatal ages. This work identifies a novel role for microglia in the postnatal maturation of retinal photoreceptors.