bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of Three‐Dimensional Magnetic Resonance Elastography in the Diagnosis of Nonalcoholic Steatohepatitis in Obese Patients Undergoing Bariatric Surgery
Authors: Allen Alina M. , Shah Vijay H. , Therneau Terry M. , Venkatesh Sudhakar K. , Mounajjed Taofic , Larson Joseph J. , Mara Kristin C. , Schulte Phillip J. , Kellogg Todd A. , Kendrick Michael L. , McKenzie Travis J. , Greiner Suzanne M. , Li Jiahui , Glaser Kevin J. , Wells Michael L. , Chen Jun , Ehman Richard L. , Yin Meng 
Published: December 2018

The lack of reliable, noninvasive methods to diagnose early nonalcoholic steatohepatitis (NASH) is a major unmet need. We aimed to determine the diagnostic accuracy of three‐dimensional magnetic resonance elastography (3D‐MRE), with shear stiffness measured at 60 Hz, damping ratio at 40 Hz, and magnetic resonance imaging proton density fat fraction (MRI‐PDFF) in the detection of NASH in individuals undergoing bariatric surgery. Obese adults at risk for NASH were enrolled between 2015 and 2017 (prospective cohort, n = 88) and 2010 and 2013 (retrospective cohort, n = 87). The imaging protocol consisted of multifrequency 3D‐MRE (mf3D‐MRE) with shear waves delivered at different frequencies to explore parameters that best correlated with histologic NASH, and MRI‐PDFF to estimate steatosis. The prospective cohort was used to establish the optimal mf3D‐MRE technical parameters for NASH detection. The two cohorts were then combined to derive predictive models of NASH and disease activity by nonalcoholic fatty liver disease activity score (NAS) using the three imaging parameters that correlated with NASH. A total of 175 patients (median age 45, 81% women, and 81 [46%] with histologic NASH) were used for model derivation. From the complex shear modulus output generated by mf3D‐MRE, the damping ratio at 40 Hz and shear stiffness at 60 Hz best correlated with NASH. The fat fraction obtained from MRI‐PDFF correlated with steatosis (P < 0.05 for all). These three parameters were fit into a logistic regression model that predicted NASH with cross‐validated area under the receiver operating characteristic curve (AUROC) = 0.73, sensitivity = 0.67, specificity = 0.80, positive predictive value = 0.73 and negative predictive value = 0.74, and disease activity by NAS with cross‐validated AUROC = 0.82. Conclusion: The mf3D‐MRE allows identification of imaging parameters that predict early NASH and disease activity. This imaging biomarker represents a promising alternative to liver biopsy for NASH diagnosis and monitoring. The results provide motivation for further studies in nonbariatric cohorts.
Introducing a new collaborative prenatal clinic model
Authors: Rule Travis , Beckmann Michael 
Published: December 2018

AbstractObjectiveTo measure quality of care‐planning for women in a new collaborative prenatal model of care.MethodsA collaborative patient‐centered (CPC) model was introduced into a tertiary prenatal clinic, replacing the traditional model. A retrospective analysis was conducted of women with a first documented hospital doctor contact between February 1, 2015, and October 1, 2016. Quality of care‐planning before and after introduction of the model was compared by measuring the number and size of documented care plans and amount of doctor contact. Categorical data were compared using χ2 or Fisher exact tests; continuous data were analyzed using the student t test.ResultsQuality of care was measured for 3606 women before, and 3511 after, introduction of the CPC model. Under the model, more women had clinical risks identified and care plans documented (2224 [63.3%] vs 1883 [52.2%]; P<0.001), and more clinical risks were identified (1.07 vs 0.74; P<0.001). Doctors documented more in each care plan (P<0.001) and had more subsequent clinical contact with women (0.69 ± 1.23 vs 0.88 ± 1.34; P<0.001) in the model.ConclusionIntroduction of a collaborative model in a tertiary prenatal clinic improved identification of clinical risks and documentation of care plans, and increased doctor contacts.
Using misoprostol to treat postpartum hemorrhage in home deliveries attended by traditional birth attendants
Authors: Abbas Dina F. , Jehan Nusrat , Diop Ayisha , Durocher Jill , Byrne Meagan E. , Zuberi Nadeem , Ahmed Zafar , Walraven Gijs , Winikoff Beverly 
Published: December 2018

AbstractObjectiveTo explore the clinical and programmatic feasibility of using 800 μg of sublingual misoprostol to prevent and treat postpartum hemorrhage (PPH) during home delivery.MethodsThe present double‐blind randomized controlled trial included women who underwent home deliveries in Chitral district, Khyber Pakhtunkhwa province, Pakistan, after presenting at healthcare facilities during the third trimester of pregnancy between May 28, 2012, and November 27, 2014. Participants were randomized in a 1:1 ratio to receive either 800 μg of misoprostol or placebo sublingually if PPH was diagnosed, having previously received a prophylactic oral dose of 600 μg misoprostol. The primary outcome, hemoglobin decrease of 20 g/L or greater from pre‐ to post‐delivery assessment, was compared on a modified intention‐to‐treat basis.ResultsThere were 49 patients allocated to receive misoprostol and 38 allocated to receive placebo; the incidence of a 20 g/L decrease in hemoglobin was similar between the groups (20/43 [47%] vs 19/33 [58%], respectively; P=0.335).ConclusionThere was no significant difference in clinical outcomes between the two trial arms.ClinicalTrials.gov:NCT01485562.
Timing of staples and dressing removal after cesarean delivery (the SCARR study)
Authors: Heinemann Netanella , Solnica Amy , Abdelkader Rana , Gutman Jakov , Nalbandian Noemzar , Raizman Ela , Hochner‐Celnikier Drorith 
Published: December 2018

AbstractObjectiveTo investigate optimal timing of dressing and staples removal after cesarean delivery (CD).MethodsThis prospective clustered clinical trial enrolled women undergoing CD between January 1, 2013, and October 31, 2014, at Hadassah–Hebrew University Hospital, Jerusalem. Women were assigned to one of five clusters differing in timing of dressing and staples removal. We assessed scar healing at 6 weeks.Results920 women completed telephone questionnaires. Wound healing did not differ significantly among the clusters: the healing complication rate was 21% in the control group (n=46) and ranged from 18% to 26% (n=27–50) in clusters two to five (P=0.49). More healing complications were observed in women with a body mass index (BMI) of more than 35 kg/m2 versus 35 kg/m2 or less (P=0.016), urgent versus elective CD (P=0.013), preterm premature rupture of the membranes (PPROM) versus intact membranes (P=0.016), and chorioamnionitis at delivery versus no chorioamnionitis (P=0.001). 586 (64%) women underwent physician assessment at staples removal and at 6 weeks post CD.ConclusionsTiming of dressing and staples removal has no effect on CD scar healing in low‐ and high‐risk parturients. A BMI of more than 35 kg/m2, urgent CD, PPROM, and chorioamnionitis were associated with mal‐healing, regardless of cluster.Clinicaltrials.gov: NCT01724255.
High orchiectomy and ipsilateral testicular sperm extraction in a man with a testicular tumor and Klinefelter syndrome: A case report
Authors: Takeshima Teppei , Yamamoto Mizuki , Takamoto Daiji , Mochizuki Taku , Kuroda Shinnosuke , Kawahara Takashi , Izumi Koji , Teranishi Jun‐ichi , Miyoshi Yasuhide , Otani Masako , Uemura Hiroji , Yumura Yasushi 
Published: December 2018

IntroductionTesticular epidermal cysts in Klinefelter syndrome are very rare. We report a case of Klinefelter syndrome associated with a testicular epidermal cyst. To our knowledge, this is the first report showing successful spermatozoa retrieval from the affected testis.Case presentationA 25‐year‐old married man was referred to our hospital with right scrotal induration, which was in lower pole of the right testis. Testicular cancer tumor markers were normal; endocrinological findings indicated hypergonadotropic hypogonadism. Semen analyses revealed azoospermia. Preoperative chromosome test result: 47, XXY karyotype; ultrasonography report: 1.9‐cm internal heterogeneous echoic mass in the right testis (malignancy not discarded). Because the patient hoped for children, he underwent high orchiectomy with ipsilateral testicular sperm extraction (200 spermatozoa from normal testicular tissue) for future fertilization procedures. Tumor pathology was an epidermal cyst.ConclusionWhile performing orchiectomy for testicular tumors, sperm retrieval should be attempted from normal tissues in patients planning for children.
A new method for excavating feature lncRNA in lung adenocarcinoma based on pathway crosstalk analysis
Authors: Qi Guoqiang , Kong Wei , Mou Xiaoyang , Wang Shuaiqun 
Published: December 2018

AbstractRecent theoretical and experimental studies indicate that long‐chain noncoding RNAs (lncRNAs) are essential for the growth and differentiation of cells and the occurrence and development of tumors in epigenetics, but the regulation of lncRNA on gene expression, transcriptional activation, and transcriptional interference in diseases is still unclear. There is an urgent need for effective methods to discover significant lncRNAs with their functions on gene regulatory mechanisms. For this purpose, a new method of extracting significant lncRNA based on pathway crosstalk and dysfunction caused by the differentially expressed genes in lung adenocarcinoma (LUAD) was proposed. The pathway analysis method based on global influence (PAGI) was first applied to find the feature genes that play an important role in the crosstalks of disease‐related pathways. Then to explore the hub lncRNAs, the weighted gene coexpression network analysis (WGCNA) was used to construct coexpression models of the feature genes and lncRNAs. The experiment results showed that 64 out of the 322 hub lncRNAs were closely related to the clinical features of patients with LUAD. Among them, nine lncRNAs (UCA1, LINC00857, PVT1, PCAT6, LINC00460, LINC00319, AP000553.1, AP000439.2, and AP005233.2) were identified to be tightly correlated with non‐small–cell lung cancer (NSCLC) pathways. In summary, we offer an effective way to extract significant lncRNA by dysfunctional pathway crosstalk in LUAD which allows the selected lncRNAs with more biologically interpreted and reproducible results. This method can be applied to other diseases and provide useful information for understanding the pathogenesis of human cancer.
Glutaminyl cyclase inhibitor contributes to the regulation of HSP70, HSP90, actin, and ribosome on gene and protein levels in vitro
Authors: Yu Xi , Li Yue , Zou Yongdong , Zheng Yizhi , He Zhendan , Liu Zhigang , Xie Wenlin , Wu Haiqiang 
Published: December 2018

AbstractBecause of the crucial roles of upregulated glutaminyl cyclase (QC) in the initiation and development of Alzheimer's disease (AD), QC inhibitors are supposed as disease‐modifying agents for the treatment of AD. And reported compounds encourage this hypothesis greatly based on the remarkable anti‐AD effects in vivo. To illustrate the mechanism in detail, the actions of a selected QC inhibitor (23) were assessed firstly in a cell system here. It was demonstrated that QC activities and the generation of pyroglutamate‐modified β‐amyloids in PC12 cells were both inhibited obviously after the treatment of 23. A total of 13 and 15 genes were up‐ and downregulated significantly in treated cells by RNA‐sequencing analysis. Quantitative real‐time polymerase chain reaction, enzyme‐linked immunosorbent assay, WB, and immunoﬂuorescence analysis supported the effects of 23 on the transcriptome of PC12 cells consequently. The expressions of chaperones, heat shock proteins (HSP) 70, and 90, were upreglutated, while gene expression of actin and the level of encoded protein were reduced significantly in PC12 cells with the treatment. Furthermore, the regulations of ribosome were observed after the treatment. These results indicate the potency of 23 to improve the translation, expression and folding regulation of proteins and affect the multivalent cross‐linking of cytoskeletal protein and other proteins subsequently in the cell system and might contribute to the understanding of the mechanism of QC inhibitor as potential anti‐AD agents.
CXCL12/CXCR4 promotes proliferation, migration, and invasion of adamantinomatous craniopharyngiomas via PI3K/AKT signal pathway
Authors: Yin Xiaohong , Liu Zhiyong , Zhu Pan , Wang Yuelong , Ren Qingqing , Chen Hongxu , Xu Jianguo 
Published: December 2018

AbstractObjectivesAdamantinomatous craniopharyngiomas (adaCP) accounts for 5.6% to 15% of intracranial tumors. High expression of chemokine (C‐X‐C motif) ligand 12 (CXCL12, also known as stromal cell‐derived factor 1 [SDF1]) and its receptor CXC receptor type 4 (CXCR4) are widespread in various malignancy via multiple signal transduction pathways. This study aims to investigate the mechanism of CXCL12/CXCR4 promoting proliferation, migration, and invasion of adaCP.MethodsQuantitative real‐time polymerase chain reaction, Western blot analysis, and immunohistochemistry were used to evaluate the expression of CXCL12/CXCR4 mRNA and protein in 10 human adaCP tissues. Three successfully primary cell lines were obtained from native mainly solid tumor specimens, and confirmed by the means of inverted contrast microscope directly and following hematoxylin and eosin staining. Immunofluorescence was used to detect protein expression in vivo for the verification of primary cell line. Proliferation, migration, and invasion assays were performed to assess the biological functional role of CXCL12/CXCR4 in adaCP. The signal pathways involved in the action of CXCL12/CXCR4 in adaCP were also evaluated.ResultsCXCL12 and CXCR4 were highly expressed in human adaCP samples. Primary adaCP cells were isolated and detected by the means of immunofluorescence for the detection of pan cytokeratin (pan‐CK) and vimentin (VIM). Overexpression of CXCL12/CXCR4 significantly promoted the proliferation, migration, and invasion of primary adaCP cells. Moreover, cancer‐promoting activity of CXCL12/CXCR4 is partially through its facilitation of PI3K/AKT signal pathway.ConclusionsOur data showed that CXCL12/CXCR4 promotes adaCP proliferation, migration, and invasion through PI3K/AKT signal pathway. These findings suggested that therapeutic strategies regulating CXCL12/CXCR4 expression may provide an effective treatment of adaCP.
MicroRNA‐329‐mediated PTTG1 downregulation inactivates the MAPK signaling pathway to suppress cell proliferation and tumor growth in cholangiocarcinoma
Authors: Hu Zhi‐Gao , Zheng Chao‐Wen , Su Hui‐Zhao , Zeng Yong‐Lian , Lin Cheng‐Jie , Guo Zhen‐Ya , Zhong Fu‐Di , Yuan Guan‐Dou , He Song‐Qing 
Published: December 2018

AbstractCholangiocarcinoma (CCA) is a severe malignancy usually producing a poor prognosis and high mortality rate. MicroRNAs (miRNAs) have been reported in association with CCA; however, the role miR‐329 plays in the CCA condition still remains unclear. Therefore, this study was conducted to explore the underlying mechanism of which miR‐329 is influencing the progression of CCA. This work studied the differential analysis of the expression chips of CCA obtained from the Gene Expression Omnibus database. Next, to determine both the expression and role of pituitary tumor transforming gene‐1 (PTTG1) in CCA, the miRNAs regulating PTTG1 were predicted. In the CCA cells that had been intervened with miR‐329 upregulation or inhibition, along with PTTG1 silencing, expression of miR‐329, PTTG1, p‐p38/p38, p‐ERK5/ERK5, proliferating cell nuclear antigen (PCNA), Cyclin D1, Bcl‐2‐associated X protein (Bax), B‐cell CLL/lymphoma 2 (Bcl‐2), and caspase‐3 were determined. The effects of both miR‐329 and PTTG1 on cell proliferation, cell‐cycle distribution, and apoptosis were also assayed. The miR‐329 was likely to affect the CCA development through regulation of the PTTG1‐mediated mitogen‐activated protein kinase (MAPK) signaling pathway. The miR‐329 targeted PTTG1, leading to inactivation of the MAPK signaling pathway. Upregulation of miR‐329 and silencing of PTTG1 inhibited the CCA cell proliferation, induced cell‐cycle arrest, and subsequently promoted apoptosis with elevations in Bax, cleaved caspase‐3, and total caspase‐3, but showed declines in PCNA, Cyclin D1, and Bcl‐2. Moreover, miR‐329 was also found to suppress the tumor growth by downregulation of PTTG1. To summarize, miR‐329 inhibited the expression of PTTG1 to inactivate the MAPK signaling pathway, thus suppressing the CCA progression, thereby providing a therapeutic basis for the CCA treatment.
DDN‐AS1‐miR‐15a/16‐TCF3 feedback loop regulates tumor progression in cervical cancer
Authors: Liu Zhihui , Wu Meiqin , Shi Huifeng , Huang Chong , Luo Sukun , Song Xiaojie 
Published: December 2018

AbstractCervical cancer (CC) is known as one of the most common gynecological tumors. Long noncoding RNAs (lncRNAs) are a group of regulators that have been widely reported in human malignant tumors including CC. On the basis of the data of The Cancer Genome Atlas, lncRNA DDN and PRKAG1 antisense RNA 1 (
DDN‐AS1) that is overexpressed in CC tissues predicted poor prognosis for patients with CC. Moreover, quantitative reverse transcription PCR analysis further identified the upregulation of DDN‐AS1 in CC tissues and cell lines. Loss‐of‐function assays revealed that knockdown of DDN‐AS1 suppressed CC progression by efficiently inhibiting cell proliferation, migration, and invasion. Mechanism investigations revealed that DDN‐AS1 was upregulated by its upstream transcription activator transcription factor 3 (
TCF3). Moreover, DDN‐AS1 increased the expression of
TCF3 by competitively binding miR‐15a and miR‐16. In conclusion, DDN‐AS1‐miR‐15a/16‐TCF3 feedback loop contributes to cell proliferation, migration, and invasion in CC.
Chlorogenic acid inhibits osteosarcoma carcinogenesis via suppressing the STAT3/Snail pathway
Authors: Zhang Fang , Yin Guodong , Han Xiufu , Jiang Xinqiang , Bao Zhongsheng 
Published: December 2018

AbstractChlorogenic acid (CA) is a polyphenol compound that possesses anticancer effects on several types of tumors. However, there are few previous studies concerning the protective effects of CA on osteosarcoma. The current study aimed to examine the toxicity of CA to osteosarcoma cells and to explore the potential mechanisms. Cell growth was evaluated using cell counting kit‐8 assay and Western blot analysis of proliferating cell nuclear antigen (PCNA). Apoptosis was assessed by flow cytometry analysis using flow cytometry and caspase‐3/7 activity assay. The expression changes of the signal transducer and activator of transcription 3 (STAT3)/Snail pathway were detected by Western blot analysis. We found that CA dose‐dependently inhibited cell viability and PCNA expression in osteosarcoma cells. Meanwhile, CA treatment increased the apoptotic rate and caspase‐3/7 activity in osteosarcoma cells in a concentration‐dependent manner. We found that CA concentration‐dependently inhibited the activation of the STAT3/Snail pathway in osteosarcoma cells. Inhibition of the STAT3/Snail pathway by si‐STAT3 retarded the growth and induced apoptosis of osteosarcoma cells. Mechanistically, activation of the STAT3/Snail pathway by pcDNA‐STAT3 reversed the effects of CA on osteosarcoma cell growth and apoptosis. In conclusion, CA inhibited osteosarcoma carcinogenesis by suppressing osteosarcoma cell growth and inducing apoptosis, which was involved in inactivation of the STAT3/Snail pathway. Therefore, our study suggested that CA might have good therapy prospects in osteosarcoma therapy.
The effects of human menstrual blood stem cells‐derived granulosa cells on ovarian follicle formation in a rat model of premature ovarian failure
Authors: Manshadi Marjan D. , Navid Shadan , Hoshino Yumi , Daneshi Erfan , Noory Parastoo , Abbasi Mehdi 
Published: December 2018

AbstractMany studies have reported that human endometrial mesenchymal stem cells (HuMenSCs) are capable of repairing damaged tissues. The aim of the present study was to investigate the effects of HuMenSCs transplantation as a treatment modality in premature ovarian failure (POF) associated with chemotherapy‐induced ovarian damage. HuMenSCs were isolated from menstrual blood samples of five women. After the in vitro culture of HuMenSCs, purity of the cells was assessed by cytometry using CD44, CD90, CD34, and CD45 FITC conjugate antibody. Twenty‐four female Wistar rats were randomly divided into four groups: negative control, positive control, sham, and treatment groups. The rat models of POF used in our study were established by injecting busulfan intraperitoneally into the rats during the first estrus cycle. HuMenSCs were transplanted by injection via the tail vein into the POF‐induced rats. Four weeks after POF induction, ovaries were collected and the levels of Amh, Fst, and Fshr expression in the granulosa cell (GC) layer, as well as plasma estradiol (E2) and progesterone (P4) levels were evaluated. Moreover, migration and localization of DiI‐labeled HuMenSCs were detected, and the labeled cells were found to be localized in GCs layer of immature follicles. In addition to DiI‐labelled HuMenSCs tracking, increased levels of expression of Amh and Fshr and Fst, and the high plasma levels of E2 and P4 confirmed that HuMenSC transplantation had a significant effect on follicle formation and ovulation in the treatment group compared with the negative control (POF) group.
Effect of EDTA, sodium, and calcium hypochlorite on the inorganic component of root canal dentin: A SEM analysis
Authors: Cardoso Luana Roleto , Baldasso Flávia Emi Razera , Delai Débora , Montagner Francisco , Kopper Patrícia Maria Poli 
Published: December 2018

AbstractThis study evaluated the influence of canal irrigation protocols with 5.25% calcium hypochlorite [Ca(OCl)2], 5.25% sodium hypochlorite (NaOCl) and 17% EDTA solution on the inorganic component of root canal dentin. Sixty roots were randomly divided into six groups (n = 10): Saline solution (SS) (control); Saline solution (SSE) +17% EDTA; CH − 5.25% Ca(OCl)2; CHE − 5.25% Ca(OCl)2 + 17% EDTA; SH − 5.25% NaOCl; SHE − 5.25% NaOCl +17% EDTA. After canal irrigation, the specimens were longitudinally split and analyzed by scanning electron microscopy (SEM). Dentinal tubules were observed in transverse (middle and apical thirds) direction. The images were classified in scores, according to smear layer removal and peritubular dentin alteration, and data were analyzed by Kruskal–Wallis's test, followed by Dunn's test (α = 0.05). In the middle third, all groups with EDTA presented better removal of the smear layer with higher peritubular dentin alteration (p < .05). In the apical third, this happened only in SHE (p < .05). Ca(OCl)2 and NaOCl exhibit similar performance to remove smear layer and alteration of the inorganic component in the middle third. In the apical third, NaOCl associated with EDTA showed better performance in smear layer removal.