bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular vesicle RNAs reflect placenta dysfunction and are a biomarker source for preterm labour
Authors: Fallen Shannon , Baxter David , Wu Xiaogang , Kim Taek‐Kyun , Shynlova Oksana , Lee Min Young , Scherler Kelsey , Lye Stephen , Hood Leroy , Wang Kai 
Published: March 2018

AbstractPreterm birth (PTB) can lead to lifelong complications and challenges. Identifying and monitoring molecular signals in easily accessible biological samples that can diagnose or predict the risk of preterm labour (PTL) in pregnant women will reduce or prevent PTBs. A number of studies identified putative biomarkers for PTL including protein, miRNA and hormones from various body fluids. However, biomarkers identified from these studies usually lack consistency and reproducibility. Extracellular vesicles (EVs) in circulation have gained significant interest in recent years as these vesicles may be involved in cell‐cell communication. We have used an improved small RNA library construction protocol and a newly developed size exclusion chromatography (SEC)‐based EV purification method to gain a comprehensive view of circulating RNA in plasma and its distribution by analysing RNAs in whole plasma and EV‐associated and EV‐depleted plasma. We identified a number of miRNAs in EVs that can be used as biomarkers for PTL, and these miRNAs may reflect the pathological changes of the placenta during the development of PTL. To our knowledge, this is the first study to report a comprehensive picture of circulating RNA, including RNA in whole plasma, EV and EV‐depleted plasma, in PTL and reveal the usefulness of EV‐associated RNAs in disease diagnosis.
Cell‐free lncRNA expression signatures in urine serve as novel non‐invasive biomarkers for diagnosis and recurrence prediction of bladder cancer
Authors: Du Lutao , Duan Weili , Jiang Xiumei , Zhao Li , Li Juan , Wang Rui , Yan Suzhen , Xie Yujiao , Yan Keqiang , Wang Qingliang , Wang Lili , Yang Yongmei , Wang Chuanxin 
Published: March 2018

AbstractCell‐free long non‐coding RNAs (lncRNAs) are stably present in urine and can serve as non‐invasive biomarkers for cancer. We aimed to identify signatures of lncRNAs in urine for diagnosis and prognosis of bladder cancer (BC). Screening of lncRNAs by microarray analysis was performed using urine samples of 10 BC patients and 10 controls. Expressions of candidate lncRNAs were evaluated in the training and validation set including 230 BC patients and 230 controls by quantitative reverse transcription polymerase chain reaction (qRT‐PCR). A two‐lncRNA panel (uc004cox.4 and GAS5) was constructed and provided high diagnostic accuracy of BC with an area under the curve (AUC) of 0.885 (95% CI, 0.836‐0.924). The AUCs of the lncRNA panel for Ta, T1 and T2‐T4 were 0.843, 0.867 and 0.923, respectively, significantly higher than those of urine cytology (all P < .05). Kaplan‐Meier analysis revealed that higher level of uc004cox.4 was associated with poor recurrence‐free survival (RFS) of non‐muscle invasive BC (NMIBC) (P = .008). Additionally, Cox regression analysis indicated that uc004cox.4 was an independent prognostic factor for RFS of NMIBC (P = .018). Taken together, our findings indicated that urinary lncRNA signatures possessed potential clinical value for BC diagnosis. Moreover, uc004cox.4 could provide prognostic information for NMIBC.
Jenynsia lineata species complex, revision and new species description (Cyprinodontiformes: Anablepidae)
Authors: F. Amorim P. 
Published: March 2018

The Jenynsia lineata species complex comprises J. lineata from Montevideo, Uruguay and Jenynsia multidentata, from coastal basins of Brazil, Uruguay and Argentina up to 1200 m a.s.l. Taxonomic divisions within this group were tested using three different species delimitation methods, which found the two existing names to be synonyms and revealed a new cryptic species. Jenynsia darwini sp. nov. is distinguished from all congeners by having a unique combination of character states, including the shape of the dorsal postcleithrum (three times higher than wide v. less than two times higher than wide) and female colour pattern in the half of the caudal peduncle with rows of chromatophores segmented in unaligned spots (v. aligned spots forming lines). The new species also differs from J. lineata by having 26 nucleotide substitutions in the mitochondrial gene cytochrome c oxidase I (coI). Phylogenetic analysis of the genus based on morphological characters proposed by previous studies corroborates monophyly of the subgenera Plesiojenynsia and Jenynsia, with the new species being allocated to the subgenus Jenynsia as the sister group of J. lineata.
Characterization of large basking shark Cetorhinus maximus aggregations in the western North Atlantic Ocean
Authors: Crowe L. M. , O'Brien O. , Curtis T. H. , Leiter S. M. , Kenney R. D. , Duley P. , Kraus S. D. 
Published: March 2018

Cetorhinus maximus aggregations recorded during extensive aerial survey efforts off the north‐eastern United States between 1980 and 2013 included aggregations centring on sightings with group sizes of at least 30 individuals. These aggregations occurred in summer and autumn months and included aggregation sizes of up to 1398 individuals, the largest aggregation ever reported for this species. The aggregations were associated with sea surface temperatures of 13–24° C and chlorophyll‐a concentrations of 0·4–2·6 mg m−3 and during one aggregation, a high abundance of zooplankton prey was present. Photogrammetric tools allowed for the estimation of total body lengths ranging between 4 and 8 m. Characterization of these events provides new insight into the potential biological function of large aggregations in this species.
Issuing and Responding to Unusual Questions: A Conversation Analytic Account of Tom Andersen's Therapeutic Practice
Authors: Smoliak Olga , Le Couteur Amanda , Quinn‐Nilas Christopher 
Published: March 2018

Tom Andersen is considered one of the key contributors to the development of postmodern practice. Little is known, however, about the ways in which his ideas and practices are routinely carried out in situ. We used Conversation Analysis (CA) to investigate a session of couple therapy facilitated by Andersen. We show how Andersen and client participants oriented to and addressed problems of understanding that occurred between them. The source of this trouble was Andersen's use of unusual question formulations. We offer preliminary evidence that such unusual formulations served particular interactional and institutional (i.e., therapeutic) functions in their local contexts of use. We conclude by considering some implications of this analysis—and of conversation analytic inquiry more generally—for the practice of family therapy.
A National Survey Examining Manuscript Dissertation Formats Among Nursing PhD Programs in the United States
Authors: Graves Janessa M. , Postma Julie , Katz Janet R. , Kehoe Leanne , Swalling Eileen , Barbosa‐Leiker Celestina 
Published: March 2018

AbstractPurposeAmong research‐focused nursing doctoral (PhD) programs in the United States, the traditional dissertation format has recently given way to a series of publication‐ready manuscripts, often bookended by introduction and conclusion chapters. To help programs make decisions about the use of these formats, this study undertook a national survey of programs offering PhDs in nursing. The purpose of this study was to explore the advantages and disadvantages of the traditional format versus manuscript option for dissertations among nursing PhD programs in the United States.DesignCross‐sectional census survey of U.S. nursing PhD programs.MethodsA web‐based survey was administered to all U.S. nursing PhD programs. Respondents indicated formats offered, factors contributing to decisions of which formats to offer, and lessons learned. Descriptive statistics and inductive content analyses were used for analysis.FindingsOf 121 eligible institutions, 79 provided eligible responses (66.7%). The majority (59%) offered both formats; 11% offered the manuscript option only, and 24% offered the traditional format only. Faculty support (or lack thereof) contributed to adoption (or not) of the manuscript option. Respondents' approaches to the manuscript option (e.g., number of papers) and advice are summarized.ConclusionsManuscript option dissertations are commonly offered and provide benefits to students and faculty; however, thoughtful implementation is critical. Programs need to agree upon clear expectations and have graduate school support (e.g., formatting). Faculty need mentorship in advising manuscript option students who choose to use this format, and the time and support. Finally, students need additional writing skills that could be provided through coursework or via individual work with mentors.Clinical RelevanceAs nursing education continues to expand further into doctoral research, programs must examine dissertation formats in order to both prepare future nurse scholars and disseminate nursing research that is critical to improving nursing education, patient care, and clinical practice.
Opinions and attitudes on the relationship between spirituality, religiosity and health: A comparison between nursing students from Brazil and Portugal
Authors: Cordero Rocío de Diego , Romero Bárbara Badanta , de Matos Filomena Adelaide , Costa Emília , Espinha Daniele Corcioli Mendes , Tomasso Claudia de Souza , Lucchetti Alessandra Lamas Granero , Lucchetti Giancarlo 
Published: March 2018

Aims and objectivesTo compare the opinions and attitudes of Portuguese‐speaking nursing students from Brazil and Portugal on the relationship between religiosity/spirituality and the ability to approach these issues with patients, in their undergraduate training and practice.BackgroundAlthough there are studies investigating nursing students' opinions concerning religiosity and spirituality in clinical practice, few have investigated if there are cross‐cultural differences between countries.DesignObservational, cross‐sectional and multicenter study carried out in 2010 and 2011 in Brazil and in 2016 in Portugal.MethodsA total of 260 third and fourth year nursing students (139 from Portugal and 121 from Brazil) from four nursing schools were included. Religious beliefs (Duke Religion Index), attitudes and opinions about spirituality and health (Curlin's questionnaire) were assessed. A comparison between students from both countries was carried out.Results/FindingsSignificant differences were found between nursing students from Brazil and Portugal, which are countries with the same language, but with different nursing training programs and population characteristics. Brazilian students were more religious and have stronger opinions on the influence and appropriateness of spirituality in clinical practice than Portuguese students. However, both groups of students indicated they should be prepared to address religiosity and spirituality with patients, that these subjects should be included in the curriculum and that they were not properly prepared to address spiritual issues.ConclusionAlthough different opinions and attitudes were found between Brazilian and Portuguese nursing students, more training in these issues should be implemented in the undergraduate education. Cross‐cultural studies could help fostering a broad discussion in the field.Relevance to clinical practiceThese findings could contribute to raise awareness on the importance of improving the training of relational competencies that prepare students to address the dimension of spirituality and religiosity with their patients.
Stroke survivors’ and carers’ experiences of a systematic voiding programme to treat urinary incontinence after stroke
Authors: Gibson Josephine ME , Thomas Lois H , Harrison Joanna J , Watkins Caroline L ,   the ICONS Project Team and the ICONS Patient, Public and Carer Involvement Groups
Published: March 2018

Aims and objectivesTo explore the views and experiences of stroke survivors and carers about a systematic voiding programme for poststroke incontinence.BackgroundUrinary incontinence after stroke is common and associated with poorer functional outcome. Structured assessment and management are potentially effective interventions, but it is important that there is a good understanding of stroke survivors’ and carers’ views of their acceptability and implementation.DesignA qualitative study within a feasibility trial.MethodsSixteen participants (12 stroke survivors and four carers) were interviewed using a structured schedule shortly before discharge from one of six inpatient stroke units across six hospitals. Interviews were audio‐recorded and transcribed verbatim, and thematic analysis was conducted.FindingsParticipants included seven male and five female stroke survivors (mean age 76 years) and four female carers (two wives and two daughters). Themes relating to the preliminary (assessment) phase of the programme were as follows: physical impact, psychological impact, beliefs about incontinence, and the assessment process itself. Main themes relating to the implementation of the programme included the following: timed voiding decisions, adapting the programme or the timed voiding schedule, and urinary incontinence management techniques. Participants felt that the programme helped them to re‐establish a regular pattern of micturition and to regain autonomy.ConclusionsThe effectiveness of a systematic voiding programme may partly lie in its educational component, challenging patients’ and carers’ assumptions that poststroke incontinence is inevitable. Individual adaptation of the programme and the ability to incorporate it alongside other aspects of care are likely to be key factors influencing implementation.Relevance to clinical practiceUrinary incontinence is common after stroke. To maximise benefits from a systematic voiding programme, nurses should support stroke survivors to overturn erroneous beliefs, to participate in tailoring of the programme, and in self‐management where appropriate.
Reference values for circulating pregnancy‐associated microRNAs in maternal plasma and their clinical usefulness in uncomplicated pregnancy and hypertensive disorder of pregnancy
Authors: Murakami Yuko , Miura Kiyonori , Sato Shuntaro , Higashijima Ai , Hasegawa Yuri , Miura Shoko , Yoshiura Koh‐ichiro , Masuzaki Hideaki 
Published: March 2018

AbstractAimThe aim of this study was to establish the reference values for circulating pregnancy‐associated placental microRNAs in maternal plasma and clarify their clinical significance in patients with hypertensive disorder of pregnancy (HDP).MethodsBlood samples were collected from 145 women with uncomplicated pregnancies (24, 26, 31 and 32 women at 12, 23, 30 and 36 weeks of gestation, respectively, and 32 women 1 day after delivery). Plasma concentrations of pregnancy‐associated placental microRNAs (miR‐515‐3p, miR‐517a, miR‐517c and miR‐518b) were measured by quantitative real‐time reverse‐transcription polymerase chain reaction. Reference values for each microRNA were determined by the line of best fit and 95% prediction interval and are expressed as logarithmic transformation. To clarify the clinical significance of these reference values, we measured the plasma concentrations of pregnancy‐associated microRNAs in a different population comprising 33 pregnant women with HDP and 44 women with uncomplicated pregnancies.ResultsReference values for circulating pregnancy‐associated placental microRNAs on chromosome 19 miRNA clusters showed an increasing tendency as pregnancy progressed and decreased significantly 1 day after delivery (P < 0.05). The sensitivity and specificity of each reference value were 57.6% and 93.2% for miR‐515‐3p, 63.6% and 75.0% for miR‐517a, 75.8% and 79.5% for miR‐517c and 63.6% and 75.0% for miR‐518b, respectively. The positive and negative predictive values of each reference value were 86.4% and 74.5% for miR‐515‐3p, 65.6% and 73.3% for miR‐517a, 73.5% and 81.4% for miR‐517c and 65.6% and 73.3% for miR‐518b, respectively.ConclusionEstablishing the reference values for circulating pregnancy‐associated placental microRNAs in maternal plasma could be useful for the evaluation of HDP.
Mosaic male fetus of Turner syndrome with partial chromosome Y: A case report
Authors: Xue Dan , Cao Dong‐Hua , Mu Kai , Lv Yuan , Yang Jun 
Published: March 2018

AbstractTurner syndrome, characterized by the presence of a monosomy X cell line, is a common chromosomal disorder. Patients with Turner syndrome are usually phenotypically female, and male cases are rarely reported. Here, we report a fetus with a mosaic karyotype: mos 45,X/46,X,del(Y)(q11.21). The fetus was initially misdiagnosed as female with Turner syndrome by both noninvasive prenatal testing and cytogenetic analysis of amniotic fluid and was subsequently found to have male anatomy by antenatal ultrasonography at 24 weeks gestational age. Through single nucleotide polymorphism‐array and fluorescence in situ hybridization testing, we found that there was a truncated Y chromosome with sex‐determining region Y (SRY) present in some cells of the fetus, which caused the male features in the fetus.
Clinical application of serum anti‐Müllerian hormone as an ovarian reserve marker: A review of recent studies
Authors: Iwase Akira , Osuka Satoko , Goto Maki , Murase Tomohiko , Nakamura Tomoko , Takikawa Sachiko , Kikkawa Fumitaka 
Published: March 2018

AbstractIt has been more than 15 years since the measurement of serum anti‐Müllerian hormone (AMH) first allowed the quantitative assessment of ovarian reserve. Meanwhile, the clinical implication of serum AMH has been expanding. The measurement of serum AMH has been applied in various clinical fields, including assisted reproduction, menopause, reproductive disorders and assessment of ovarian damage/toxicity. Well‐known findings about the usefulness of serum AMH revealed by numerous studies executed in the early era include decline with aging, a good correlation with oocyte yield in assisted reproduction, upregulation in polycystic ovarian syndrome and a decrease on ovarian surgery and toxic treatment. More intensive research, including a meta‐analysis, cutting‐edge clinical trial and advances in AMH assays, has yielded newer findings and firmer clinical interpretations in serum AMH in the past few years. Variations in the AMH decline trajectory in the general population do not support the accurate prediction of menopause. The ability to predict pregnancy in infertility treatment and natural conception is poor, while a nomogram integrating serum AMH as a stimulation protocol is useful for avoiding poor and/or hyper‐responses. On the other hand, improvements in measuring very low concentrations of serum AMH may be capable of distinguishing women with poor ovarian function. Age‐independent standardization of AMH values may be helpful for comparing ovarian reserves among women at different ages.
Retracted: Variation of Phomopsis viticola isolates in the interaction with grapevine germplasm
Authors: Sabetta Wilma , Montemurro Cinzia , Miazzi Monica Marilena 
Published: March 2018

Wilma Sabetta, Cinzia Montemurro and Monica Marilena Miazzi, (2019). Variation of Phomopsis viticola isolates in the interaction with grapevine germplasm. Journal of Phytopathology, 167, 312. https://doi.org/10.1111/jph.12699The above article from Journal of Phytophathology, published as an Early View article online on 08 March 2018 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, Wilma Sabetta, Cinzia Montemurro and Monica Marilena Miazzi, the journal Editor in Chief, Andreas von Tiedemann, and John Wiley and Sons Ltd. The retraction has been made as a consequence of a disagreement between the authors as to the conclusions of the study.
Nitidine chloride inhibits proliferation and induces apoptosis in ovarian cancer cells by activating the Fas signalling pathway
Authors: Chen Shipeng , Yang Luo , Feng Jie 
Published: March 2018

Abstract

Objectives
To explore the apoptotic effects and underlying mechanisms of nitidine chloride (NC) in epithelial ovarian cancer.

Methods
The MTT cell proliferation assay was used to detect the inhibitory effects of different concentrations of NC (0, 0.3125, 0.625, 1.25, 2.5, 5 and 10 μg/ml) in SKOV3 ovarian carcinoma cells. The number of apoptotic cells was observed by Hoechst staining and measured by flow cytometry. Quantitative PCR was used to measure the expression of Fas, Fas-associated death domain-containing protein (FADD), caspase-8 and caspase-3. RNA interference (RNAi) was used to determine whether caspase-8 played an important role in NC-induced apoptosis.

Key findings
Nitidine chloride inhibited the proliferation of SKOV3 cells (IC50 = 2.317 ± 0.155 μg/ml) after 24 h of treatment and induced apoptosis (15.9–64.3%). Compared with the control group, a significant increase in Fas, FADD, caspase-8 and caspase-3 gene expression was observed in the NC-treated groups (P &lt; 0.05). After silencing caspase-8 by RNAi, the antiproliferative activity and pro-apoptotic activity of NC in SKOV3 cells decreased (P &lt; 0.05).

Conclusions
Our study showed that NC induced apoptosis in SKOV3 cells by activating the Fas signalling pathway, and caspase-8 played an important role in this process.
25‐Hydroxyvitamin D3‐enhanced PTPN2 positively regulates periodontal inflammation through the JAK/STAT pathway in human oral keratinocytes and a mouse model of type 2 diabetes mellitus
Authors: Zhang P. , Zhang W. , Zhang D. , Wang M. , Aprecio R. , Ji N. , Mohamed O. , Li Y. , Ding Y. , Wang Q. 
Published: March 2018

Background and ObjectivePeriodontitis is an increasingly prevalent complication of diabetes mellitus (known as diabetes mellitus‐associated periodontitis), and 25‐hydroxyvitamin D3 (25VD3) was recently found to be a critical regulator of innate immunity in this disease, but the underlying mechanisms remain poorly understood. T‐cell protein tyrosine phosphatase non‐receptor type 2 (PTPN2) is a potential downstream protein of the 25VD3/vitamin D receptor pathway. The aim of this study was to investigate the regulation of PTPN2 in periodontal inflammation in diabetes mellitus‐associated periodontitis.Material and MethodsPorphyromonas gingivalis‐infected db/db mice were treated with 25VD3. Their fasting blood glucose and body weight were monitored every other week, and the levels of alveolar bone loss and serum inflammatory cytokines (tumor necrosis factor‐α, interferon‐γ and interleukin‐6) were determined at the time of killing. The effect of PTPN2 on human OKF6‐TERT2 oral keratinocytes was examined through the knockout of PTPN2 using the CRISPR/Cas9 knockout plasmid. The expression levels of the PTPN2, vitamin D receptor and JAK1/STAT3 signaling proteins in the gingival epithelium and OKF6‐TERT2 cells were determined through western blot and immunohistochemical analyses.ResultsAfter 25VD3 treatment, db/db mice exhibited alleviated serum inflammatory cytokines and alveolar bone loss, and 25VD3‐enhanced PTPN2 expression decreased the expression of the JAK1/STAT3 signaling proteins in the gingival epithelium. Analyses of human oral keratinocytes showed that 25VD3 increased the expression of PTPN2, which dephosphorylates protein substrates in the JAK1/STAT3 signaling pathway.ConclusionPTPN2 contributed to a decrease in periodontal inflammation in type 2 diabetes mellitus via dephosphorylate protein substrates in the JAK1/STAT3 signaling pathway after 25VD3 treatment in human oral keratinocytes and a mouse model of type 2 diabetes mellitus. A thorough understanding of PTPN2 and its involvement in inhibiting inflammation might provide alternative therapeutic approaches for the pathogenesis and treatment of diabetes mellitus‐associated periodontitis.