bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimisation of the Microbial Cell Concentration Procedure in Plague Vaccine Production
Authors: Sharov D. A. , Leshchenko A. A. , Bagin S. V. , Mokhov D. A. , Logvinov S. V. , Krupin V. V. , Ezhov A. V. , Lazykin A. G. , Birjukov V. V. 
Published: March 2019

To date, the technology of live plague vaccine production uses a combined method of concentrating microbial cells which consists of three operations with a total duration of 18 hours. The procedure of obtaining concentrate, which is used in the current vaccine production technology, has a number of disadvantages, namely: multiple operations, high energy consumption, long duration, and, as a consequence, low yield of concentrated suspension (0.04 l from 1 l of native culture). The aim of the study was to optimise the procedure of Yersinia pestis ЕV microbial cell concentration using the system for tangential flow microfiltration with the ASF-020 filter support unit. Materials and methods: the vaccine strain used in the study was Yersinia pestis ЕV derived from NIIEG cell line (the strain of the Research Institute of Epidemiology and Hygiene). Submerged cultivation of the native culture was performed using the BIOR-0.25 reactor. The content of live microbial cells was determined by cytorefractometry. Oxidative metabolism was assessed using the chronoamperometric method. Physico-chemical and immunobiological properties of the dry live plague vaccine were assessed according to the monograph FS.3.3.1.0022.15 of the State Pharmacopoeia of the Russian Federation, 14 edition. Results: the equipment’s design features made it possible to carry out membrane filtration of the microbial suspension using the BIOR-0.25 reactor as an intermediate storage unit, thereby excluding three technological stages. The total concentration of microbes in the suspension obtained by the routine and the optimised methods was not less than 120 billion microbial cells/ml. A comparative study of the effect of various hydrodynamic regimes in the working cavities of ASF-009 and ASF020 filter units did not significantly affect the morphometric and physiological properties of microbial cultures. Experimental data helped to determine the process mass balance of membrane filtration. The optimised technology gave 0.17 l yield of the concentrate from 1 l of native culture, and the process duration was reduced to 4 hours. Conclusions: the process of concentrating Y. pestis EV microbial cells during production of plague vaccines was optimised. A comparative study of morphometric and physiological properties of plague microbe cultures that was carried out during their concentration using the optimised technology did not reveal any significant differences as compared to the routine one.
Impact of Texture on Sesquioxide Distribution in Southeastern Nigerian Soils
Authors: Uzoho Bethel , Okoli Nnaemeka , Ekwugha Ugochi 
Published: March 2019

Sesquioxides which play significant roles in soil classification and dominant soil properties vary in soils with particle size fractions. Using randomized complete block design, this study was conducted in the month of June 2018 to evaluate the impact of texture on sesquioxide distribution in humid rainforest soils of Southeastern Nigeria. Four mini-pedons were dug at four landscape positions (upslope, midslope, downslope and flat surface) and triplicate soil samples taken from 0-20, 20-40 and 40-60 cm depths, summing to total of 36 samples. Crystalline and amorphous Al, Fe, Mn and Si oxides were then determined at a depth of 0-20 cm using Dithionate Citrate Bicarbonate (DCB) and ammonium oxalate reagents. Dithionate and oxalate fractions in bulk soil ranged between 9300-11,400 (Fed), 390-1580 (Feox), 4600-6700 (Ald), 660-890 (Alox), 3300-5600 (Mnd), 350-580 (Mnox) and 9600-13,500 (Sid) and 1690-1790 mg kg-1 (Siox), with dithionate superior indicating high crystallinity and low mobility of the sesquioxides. Ratios of bulk soil oxalate/dithionate fractions were low and ranged between 0.04-0.17 (Feox/Fed), 0.11-0.15 (Alox/Ald), 0.10-0.16(Mnox/Mnd) and 0.13-0.18 (Siox/Sid) and confirming their crystallinity. Low Feox/Fed ratios signified that soils were well drained (< 0.35), old (< 0.65) and highly weathered (< 0.50) with the order being a decreasing sequence of upslope > flat surface > down slope > midslope. Sesquioxide contents and reactivity in soil particle size fractions (sand, silt and clay) varied with landscapes. Regression models indicated that particle size fractions accounted for 50% of 2/3rd of the bulk soil sesquioxide concentrations and that averaged over landscapes, sand fraction was more enriched with various sesquioxides than the other soil particle size fractions. Correlations between most bulk soil sesquioxide fractions and sesquioxide fractions with selected soil properties (sand, silt, clay, moisture content, total porosity, organic matter, pH, Ca and ECEC) were significant (P < 0.05).
Role of Cisternal Drainage in Patients with Traumatic Brain Injury Undergoing Decompressive Craniectomy
Authors: Thapa Amit , Adhikari Rupendra Bahadur , KC Bidur , Shakya Bikram 
Published: March 2019

The effect of decompressive craniectomy (DC) on survival and functional outcome in traumatic brain injuries (TBI) is far from satisfactory. Additional modalities including cisternal drainage (CD) that provides good control of refractory intracranial pressure (ICP) intraoperatively need careful scrutiny.
Two centre retrospective superiority study with one centre offering only standard decompressive craniectomy (DC) i.e. Group 1 and the other centre supplementing cisternal drainage (CD) to standard DC i.e. Group 2 was conducted. Consecutive patients with traumatic brain injury with signs of brain herniation or CT scan showing mass lesion or diffuse brain edema or midline shift or with GCS less than 9 or rapid fall in GCS over 2 points with persistently raised ICP of 25 mmHg over 15 minutes between August 2012 and July 2017 were included. The primary outcome was rating on Glasgow Outcome Scale (GOS) at 6 months post operatively, with GOS (1-3) categorized as ‘Unfavorable’ and GOS (4,5) as ‘Favorable’.
Patients either received DC alone (Group 1=73 patients, 48.7%) or DC with CD (Group 2=77 patients, 51.3%). 107 (71.3%) severe, 36 (24%) moderate, and 7 (4.7%) mild head injuries cases received 72 unilateral and 78 bilateral DC. GOS 1 was observed in 32 DC only group (43.8%) and 22 DC plus CD group (28.6%) (p=0.052), an absolute risk reduction of 15.2% was found. Outcome (favorable sun favorable) against all strata of head injury severity, predominant radiological feature, laterality of surgery, and patient characteristics across the two groups were statistically not significant, however the groups were statistically significantly different on age and GCS at presentation (p=0.016 & 0.034 consecutively).
Distinct survival benefit in patients with traumatic brain injury receiving cisternal drainage during decompressive craniectomy did not translate to better functional outcome.
Analysis of risk factors predicting recurrence of chronic subdural hematoma
Authors: Yogi Nikunja , Nepal Pankaj Raj , Gongal Dinesh Nath , Devkota Upendra Prasad 
Published: March 2019

Recurrences after evacuation of chronic subdural hematoma are seen in 2-33% of cases with various surgical approaches. Various demographical, clinical, radiological, surgical and postoperative management strategies have been explored as the possible predictors of recurrence.
We performed a retrospective analysis in 160 patients with chronic subdural hematoma with an aim to analyze the post-operative recurrences and factors associated with it. Various socio demographic and clinico-radiological parameters were studied against the rate of recurrence after surgery using Chi square/Fischer Exact Test. Factors showing significant association on univarate analysis were then analysed using binary logistic regression.
The rate of recurrence of CSDH in our study was 5% and the mean duration of recurrence was 33 days. Chronic alcohol use (p=0.007) and intraoperative brain expansion ((p=0.001) were the two factors associated with recurrence of CSDH. On binary logistic regression chronic alcohol use (wald-6.467, p=0.011) and intraoperative brain expansion (wald=6.674, p=0.010) were both associated significantly with recurrence of CSDGH with an odds of 7.804 and 0.058 respectively.
Study of the Outcome Differences among the Ischemic Stroke Subtypes basedon TOAST Classificationat Tribhuvan University Teaching Hospital, Nepal
Authors: Karn Ragesh , Subedi Kamal 
Published: March 2019

The objective of this study was to explore the outcome differences among is chemic stroke sub types based on “Trial of ORG 10172 in Acute Stroke Treatment (TOAST)” system in a tertiary referral hospital.
A hospital based prospectiv elongitudinal study was conducted involving 182 patients. Modified Rank in Scale (MRS) was used to assess functional out come, Kaplan-Meierproduct- limit method and Coxproportional hazards regression analys is were used to evaluate rates and identify predictors of survival and recurrent stroke.
MRS were statistically different across stroke subtypesat 30 days and 6 months (p<0.0001 and (p<0.0001) respectively). Lacunar stroke was associated with milder deficits. Estimated rates of recurrent stroke were significantly different at 30 days (p<0.0001) and at 6 months (p=0.009). Before adjusting forage, sex, stroke severity, and diabetes mellitus, infarct subtype was not an independent determinant of recurrent stroke within 30 days (p=0.057);but was at 6 months (p=0.024), how ever after same adjustment it was independent determinant both at 30 days (p=0.030) and at 6 months (p=0.025). Estimated death rates were significantly different both a t 3 0 days (p=0.026) and at 6 months (p=0.009). Ischemic stroke subtype was an independent determinant of 6-month survival both before (p=0.016) and after (p=0.027) adjustment for different parameters; however, was not of 30-day survival both before (p=0.054) and after (p=0.993) same adjustment.
Early recurrencerates were highest among large artery atherosclerotic stroke. Lacunar stroke has better post stroke functional status. Survival is poorest among cardioembolic stroke.
Demographic characteristics of stroke in a tertiary care hospital in Nepal
Authors: Dhungana Krishna 
Published: March 2019

Our aim is to find out the demographics of the patients with stroke who undergo in-patient rehabilitation in a tertiary care hospital setting. We also aim to find the frequency of different types of stroke and the frequency of stroke in different vascular territories.
Using data collected prospectively over a period of 1 year, we studied 180 individuals admitted with stroke. The demographic features of patients with stroke were collected and analyzed. Stroke was broadly stratified into hemorrhagic and ischemic stroke. Furthermore, ischemic stroke was subdivided according to the vascular territories involved: anterior cerebral artery, middle cerebral artery (MCA), posterior cerebral artery, brain-stem, cerebellar, smallvessel strokes and stroke occurring in more than one vascular territory. The most common risk factor was hypertension which was present in 50% of patients.
The most common ischemic stroke groups were MCA stroke (39.4%) and small vessel stroke (17.2%). The most common type of hemorrhage was basal ganglia hemorrhage which was present in 15% of patients.
In regards with Western literature, patients with stroke in our setting also had similar risk factors.
The proportion of hemorrhagic stroke in our country was higher than in the western world. A large proportion of patients were in the age group less than 65. However, the frequency of distribution of different types of stroke and frequency of stroke in different territories were similar.
Tribological performance and corrosion behavior of aluminum alloy protected by Cr-doped diamond-like carbon thin film
Authors: Yang Xiaoyu , Xu Hong , Mao Hongkui , Wang Gang , Liu Wei , Gao Wei , Zhu Lideng 
Published: March 2019

AbstractA gradient layer of Al/Cr diamond-like carbon film has been prepared by magnetron sputtering on 2024 Al-alloy. Emphasis has been placed on the anti-corrosion and tribological properties of the Cr-doped diamond-like film. Experimental research: scanning electron microscopy, X-ray photoelectron spectroscopy, Raman spectrometry, nano-indentation testing, potentiodynamic polarization, electrochemical impedance spectroscopy and tribological tests were undertaken to investigate the surface morphology, surface structures, chemical composition, anti-corrosion and mechanical properties of the as-prepared gradient diamond-like carbon film. The results indicated that the Cr-doped diamond-like carbon film with intermediate transition chromium layer showed improvements with regards to hardness and corrosion prevention due to the dense surface structure and decreased electrical conductivity. Cr-doped diamond-like carbon film also displayed excellent tribological properties such as a low friction coefficient and wear rate during tribological testing.
The Elements of Conceptual Model Goods and Services Tax Registered Persons in Malaysia
Authors: Liang Lee Heng , Alifiah Mohd Norfian , Chen Loo Ern 
Published: March 2019

Goods and Services Tax (GST) system was implemented on the 1st April 2015 in Malaysia. Like many others tax administration, tax compliance has been a major concern to the Royal Malaysia Customs Department (RMCD) (Zainol et al., 2015). The issue of tax non- compliance such as over claiming of input tax, not issuing tax invoices, and under declaring output tax by the registered persons induced this study. Thus, this study seeks to establish the determinant factors that may influence the tax compliance behaviour of GST registered person in Malaysia. This study also attempts to propose a conceptual framework GST tax compliance behaviour by integrating economic factors such as tax structure system of GST tax rate, audit, penalty/fine, and psychological and sociological factors like attitude towards GST and GST tax knowledge.
The Effects of Job Performance, Work Life Balance and Organizational Justice on Career Satisfaction
Authors: Saraih Ummi Naiemah , Mohd Karim Khofizhoah , Sakdan Mohd  Fo'ad 
Published: March 2019

This research is devoted to identify the effects of several factors on career satisfaction in the context of manufacturing sector. The purpose of this research is to reveal the effects of job performance, work life balance and organizational justice on employees' career satisfaction. To analyse this effects, participants from manufacturing company in Penang have been taken as the sample of this research survey. Quantitative method is adopted, and this research received 62% responses out of 240 sample size. Findings of this research presented that there were significant effects of job performance, work life balance and organizational justice on career satisfaction among employees in this company. Organizations always have quest of finding the unique ways to enhance career satisfaction of the employees. Thus, this research would help to suggest on how an adequate job performance, work life balance and organizational justice would help to increase the level of employees' career satisfaction.
O Controle de Qualidade em Mamografia e o INCA: Aspectos Históricos e Resultados
Authors: De Araújo Anna Maria Campos , Peixoto João Emílio , Da Silva Sonia Maria , Travassos Leonardo Vieira , De Souza Ricardo José , Marin Alfredo Viamonte , Canella Ellyete de Oliveira 
Published: March 2019

Introdução: Programas de rastreamento mamográfico exigem o controle da qualidade dos exames e uso seguro da radiação. No Brasil, o Instituto Nacional de Câncer José Alencar Gomes da Silva (INCA) acompanhou esse processo. Objetivo: Descrever o contexto histórico do controle das doses e da qualidade da mamografia no Brasil sob a perspectivado INCA e os resultados obtidos por dois programas de qualidade de abrangência nacional. Método: Pesquisa descritiva, de abordagem mista, utilizando documentos e publicações relacionadas ao controle de qualidade em mamografia e resultados dos programas do INCA e do Colégio Brasileiro de Radiologia e Diagnóstico por Imagem (CBR) entre 2009 e 2016. Resultados: A pesquisa documental descreve as ações de controle de qualidade da mamografia desde a década de 1970 até o ano de 2012. Entre 2009 e 2016, foram realizadas 1.156 medidas de dose em 738 serviços e 2.633 avaliações da qualidade dos exames em 390 serviços. O valor médio da dose glandular média foi de 1,81 mGy por incidência, com 22,7% das avaliações acima dos valores de referência. Em relação à qualidade dos exames, 14,0% não estavam conformes quanto aos critérios clínicos de qualidade da imagem, 5,8% quanto aos critérios físicos e 16,7% quanto à classificação BI-RADS®. Conclusão: A análise documental revela marcos importantes da qualidade da mamografia nas últimas décadas. Os resultados dos programas do INCA e do CBR fornecem informações relevantes para o desenvolvimento de ações dirigidas ao controle da dose e da qualidade da imagem e dos laudos em mamografia.
PENGARUH STIMULUS KUTANEUS SLOW-STROKE BACK MASSAGE TERHADAP INTENSITAS NYERI PADA PENDERITA LOW BACK PAIN (LBP) DI WILAYAH KERJA PUSKESMAS KURAI TAJI KECAMATAN PARIAMAN SELATAN
Authors: Mkes Syahrul Skm , Mkes Naimah Hayati 
Published: March 2019

<p><strong>ABSTRAK</strong><strong>:</strong> Angka kejadian LBP hampir sama pada semua populasi masyarakat di seluruh dunia, baik di negara maju maupun di negara berkembang. Diperkirakan setidaknya 70% manusia menderita sakit punggung, baik kronis maupun sporadis. Penelitian ini berjenis kuantitatif dengan menggunakan rancangan penelitian metode quasi eksperimen. Pada desain penelitian ini terdapat kelompok intervensi tanpa kelompok kontrol. Pendekatan desain penelitian dengan <em>one group pretest – posttest. </em>Penelitian bertujuan untuk mengetahui pengaruh stimulus kutaneus <em>slow-stroke back massage</em> terhadap intensitas nyeri pada penderita <em>Low Back Pain</em> (LBP) di wilayah kerja Puskesmas Kurai Taji Kecamatan Pariaman Selatan. Penelitian dilaksanakan di wilayah kerja Puskesmas Kurai Taji Kecamatan Pariaman Selatan pada tanggal 2 oktober 2017 s/d 27 Oktober 2017. Populasi dan sampel berjumlah 28 responden dengan tehnik <em>total sampling. </em>Hasil penelitian rata-rata intensitas nyeri LBP sebelum pemberian stimulus kutaneus <em>slow-stroke back massage</em> adalah 4,64 (skala nyeri sedang) dengan skala nyeri tertinggi 8 dan terendah 2 dan sesudah pemberian stimulus kutaneus <em>slow-stroke back massage</em> adalah 2,21 (skala nyeri ringan) dengan skala nyeri tertinggi 5 dan terendah 0. Terdapat pengaruh stimulus kutaneus <em>slow-stroke back massage</em> terhadap intensitas nyeri pada penderita <em>Low Back Pain</em> (LBP) di wilayah kerja Puskesmas Kurai Taji Kecamatan Pariaman Selatan dengan nilai p 0,000. Diharapkan pihak Puskesmas Kurai Taji Kecamatan Pariaman Selatan untuk dapat menyampaikan dan mensimulasikan tehnik stimulus kutaneus <em>slow-stroke back massage </em>pada penderita LBP yang berkunjung ke puskesmas sehingga intensitas nyeri penderita dapat berkurang saat melakukan tehnik tersebut di rumahnya dan diharapkan pihak Puskesmas dapat menyediakan ruangan dan mengaplikasikan pelaksanaan tehnik <em>slow-stroke back massage </em>sebagai salah satu cara untuk mengatasi keluhan LBP pada penderita saat berkunjung ke puskesmas.<strong></strong></p>
النشرة الإخبارية الألكترونية لوزارة التعليم العالي في الصحافة العراقية
Authors: ثامر علياء قاسم 
Published: March 2019

يهدف البحث إلى معرفة صدى النشرة الاخبارية الألكترونية لوزارة التعليم العالي والبحث العلمي في جرائد )التآخي، والزمان، والعدالة، والصباح، وبغداد) للمدة من2/10-1/11/2011، لإيضاح فاعليتها وإفادة الجرائد منها، وبيان الأهمية التي أولتها الجرائد محل الدراسة لموضوعات الاخبار في النشرة، فضلا عن إظهار الفروقات النسبية في مدى اهتمام الجرائد بموضوعات الاخبار المنشورة، والتوصل إلى نتائج تقودنا إلى استنتاجات سليمة لخدمة صاحب القرار وفتح آفاق جديدة أمام الباحثين للتوسع في دراسة هذا الموضوع.
وانتهجت الباحثة المنهج الوصفي في دراسة وتحليل الاخبار المنشورة كمياً وكيفياً، إذ اسفر التحليل الكمي الذي اعتمد الحصر الشامل عن وجود (208) تكرارات للاخبار، اخضعت للتحليل ليتم استخراج (18) فئة (موضوعاً) على وفق الوحدة الطبيعية للمادة الاعلامية.
وبعد تحليل البيانات احصائياً، خلص البحث إلى جملة من الاستنتاجات اهمها:

كان لاخبار النشرة صدى في الجرائد محل الدراسة، إذ أبدت الجرائد اهتماماً متفاوتاً، ما يشير الى تباين إفادتها منها، وهذا التباين يمكن ان تفرضه طبيعة الوسيلة الاعلامية، وسياستها العامة وفلسفتها ازاء شؤون التعليم العالي في العراق.
ركزت الجرائد الخمسة على فئة قرارات بشأن الطلبة، اذ فاقت على الفئات الاخرى بالاهتمام، ما يفسر معيارية الجرائد في انتقائها الاخبار الأكثر استقطابا للجمهور، الذي يتعامل مع المؤسسة التعليمية.
ضآلة اهتمام الجرائد بالاخبار التي تعنى بالموضوعات الثقافية والرياضية، ويعزى ذلك الى امكانية اعطاء الجرائد الاولوية للموضوعات الاخرى في ظل محدودية المساحة وكثرة اخبار النشرة، فضلا عن ان اخبار هذه النشاطات قد تعدها الجرائد لا تستقطب اهتمام القراء.
اظهرت الجرائد اهتماما دون المستوى المتوسط باخبار النشرة من حيث الكم، الا ان الباحثة تعد هذه نتيجة جيدة جدا، فالجريدة الأقل اهتماما تنشر بمعدل خبر واحد يومياً، ما يلفت الى فاعلية أداء المحررين في إعداد النشرة الاخبارية الألكترونية من حيث الكم والنوع، فضلا عن إدارتها، الامر الذي حقق تكاملاً نسبياً مع الجرائد في عملهما.
Bacteriological and ecfX- gene specific PCR based identification of Pseudomonas aeruginosa isolated from Chittagong industrial area and characterization of its extracellular amylase
Authors: Talukder Asma , Mobashshera Tanha Amina , Ahmed Istiaq Uddin , Islam Nazneen Naher 
Published: March 2019

Amylases are the starch degrading enzymes with applications ranging from food, fermentation, textiles to paper industries. In this study, a soil bacterium from the Chittagong industrial area was screened primarily for amylase activity using starch agar media. An attempt has been made to isolate and identify this amylolytic bacterium, optimization of its amylase production and characterization of the crude enzyme. The isolate was identified as Pseudomonas aeruginosa based on microscopic and biochemical tests. Beside this a speciesspecific gene ecfX (146 bp) for P. aeruginosa was amplified by PCR to confirm the identification of the isolate. A broad range of parameters for the optimization of enzyme production based on selection of best carbon and nitrogen sources, optimum pH, temperature, incubation period and effect of additional metal ions on culture media were investigated. Best enzyme activity (343.60 U/mL) was found in the presence of glucose as carbon source with yeast extract as nitrogen source at temperature 37°C after 24h at pH 9 in addition of Mg++ in submerged fermentation broth culture. The Mg++, SDS and Ca++ increased the amylase activity whereas EDTA and Zn++ were found as the inhibitory agents for amylase.
Jahangirnagar University J. Biol. Sci. 7(2): 99-114, 2018 (December)
Rediscovery of Geophila repens (L.) I.M. Johnst. (Rubiaceae) in Bangladesh
Authors: Khanam Robayda , Khan Saleh Ahammad , Rahim Abdur 
Published: March 2019

Abstract not available
Jahangirnagar University J. Biol. Sci. 7(2): 121-125, 2018 (December)
El patrimonio arquitectónico en la comarca de los Pedroches
Authors: Fernández Martín M.ª Mercedes 
Published: March 2019

La comarca de los Pedroches, dada su trayectoria histórica, conserva interesantes muestras de arquitectura. Entre los ejemplos más significativos está la desarrollada por los Hernán Ruiz, padre e hijo, activos en la comarca durante buena parte del siglo XVI. Asimismo, conserva una interesante arquitectura popular, marcada por la utilización del granito.