bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of MENAQUINONE‐7 and fat‐soluble vitamin production by starter cultures during fermentation in dairy products using RPLC method
Authors: Altuncu Vildan , Kaymaz Aykut , Ertekin Filiz Bilge , Çubuk Demiralay Ebru , Kök Taş Tuğba 
Published: October 2024

AbstractVitamin K2, also known as menaquinone, has become a significant research focus, particularly in fermented products. This study aims to investigate the content of menaquinone‐7 (MK‐7), an essential form of vitamin K, and other fat‐soluble vitamins (A, D, E) during the fermentation of various fermented milk products. The vitamin contents of six fermented milk products were analyzed: two yogurt samples (produced with commercial starter and probiotic starter), two kefir samples (produced with commercial starter and kefir grain), and milk fermented with Lactobacillus acidophilus or Bifidobacterium bifidum. Throughout the fermentation process, continuous pH monitoring was conducted, and fermentation was terminated based on pH levels. Fat‐soluble vitamins and vitamins K1, K2 were extracted from samples taken at specific fermentation periods and quantitatively determined using the reverse phase liquid chromatography (RPLC) method. The developed method was validated according to ICH guidelines. Simultaneously, the microbial content of the samples was analyzed. Among the fermented dairy products analyzed, the highest MK‐7 content (4.82 μg/100 g) was found in the kefir sample produced with kefir grain (KG). The diverse microorganisms in kefir grains necessitated detailed research to explain their role in this complex mechanism. In yogurt samples, the presence of L. acidophilus in the YB sample suggests that this bacterium may be responsible for the observed differences. This is supported by the LA sample, where production with L. acidophilus strain showed an increase from 0.97 to 1.70 at the 20th hour of fermentation. It was noted that the concentration of menaquinone‐7 increased throughout the fermentation period. Given the critical health effects of essential fat‐soluble vitamins and MKs, determining their content in commonly consumed fermented dairy products and understanding the influence of starter cultures, which are potential probiotics, on vitamin production underscores the importance of this research. It is important to highlight the potential of fermented products in nutritional recommendations.
The Impact of Vitamin E Supplementation on Oxidative Stress, Cognitive Functions, and Aging‐Related Gene Expression in Aged Mice
Authors: Molavi Vasei Farnoosh , Zamanian Mohammad Yasin , Golmohammadi Maryam , Mahmoodi Mehdi , Khademalhosseini Morteza , Tavakoli Tayyebeh , Esmaeili Ozra Sadat , Zarei Sadegh , Reza Mirzaei Mohammad , Hajizadeh Mohammad Reza 
Published: October 2024

ABSTRACTThis study investigated the effects of different doses of vitamin E on oxidative stress, cognitive function, and gene expression in aged mice. A total of 32 male mice, aged 12 months, were divided into a control group and three treatment groups. These groups received varying daily doses of vitamin E for a period of 28 days. The results showed significant improvements in cognitive function, specifically in working memory and spatial learning, in the groups that received vitamin E (100, 200, or 400 mg/kg) compared to the control group. The markers of oxidative stress and antioxidant enzyme activities also demonstrated improvements, with higher doses of vitamin E showing greater effects. The analysis of gene expression revealed increased expression of SIRT1, Nrf2, and Calstabin2, particularly at higher doses of vitamin E. These findings suggest that vitamin E supplementation may help counteract age‐related cellular changes. The study concludes that vitamin E supplementation can reduce oxidative stress, enhance cognitive function, and affect genetic markers of aging in mice, which may have therapeutic benefits in addressing age‐related cognitive decline and oxidative damage. Further research is necessary to investigate the clinical implications of these findings in humans.
Investigating the Skin Health Benefits of Rosa roxburghii, Punica granatum and Rose: A Randomized Single‐Blind Controlled Clinical Trial
Authors: Hao Yining , Wang Zhan , Qu Liping 
Published: October 2024

ABSTRACTRecent studies underscore the beneficial impacts of oral natural plant extracts on human skin health, though clinical evidence of their efficacy and safety is limited. This study evaluates the skin health effects of a novel oral supplement containing Rosa roxburghii, Punica granatum, and rose extracts (RPR) 0.70 healthy female participants were randomly assigned to either a control group or an RPR group, with the latter ingesting 20 mL of the RPR supplement daily on an empty stomach over 8 weeks. After 8 weeks, the RPR group exhibited significant enhancements (p < 0.001) in skin hydration, glossiness, elasticity, and skin tone, with increases of 69.02%, 30.48%, 25.97%, and 7.52%, respectively. Concurrently, decreases in skin firmness and melanin levels were observed at 21.17% (p = 0.007) and 25.06% (p < 0.001), respectively. Statistical analysis confirmed that these changes were significantly greater than those in the control group. Image analysis indicated no significant changes in mean optical density of hyperpigmented spots within the RPR group (p = 0.367), but a significant reduction in the areas of hyperpigmented spots, under‐eye fine lines, and crow's feet by 41.50%, 37.55%, and 29.36%, respectively (p < 0.001), whereas no significant changes were detected in the control group. Importantly, no adverse effects were observed. These findings suggest that the combined intake of Rosa roxburghii, Punica granatum, and rose extracts can improve skin health, offering a promising natural alternative for dermatological care.
Initial experience of 980‐nm diode laser treatment for recurrent non‐muscle‐invasive bladder cancer
Authors: Fukuhara Hideo , Hamada Yusei , Shimasaki Syo , Iga Ryohei , Atagi Kaya , Shigehisa Ryu , Ota Yuhi , Yamamoto Shinkuro , Osakabe Hiroto , Nao Tomoya , Shimamoto Tsutomu , Fukata Satosi , Ashida Shingo , Karashima Takashi , Inoue Keiji 
Published: October 2024

IntroductionLaser ablation using a 980‐nm wavelength diode laser, which is a new‐generation laser, for recurrent bladder cancer is known to have a lower incidence of complications and recurrence than conventional transurethral resection of bladder tumor surgery. This is the first study to report the use of 980‐nm diode laser ablation for recurrent non‐muscle‐invasive bladder cancer in Japan.Case presentationA 73‐year‐old man underwent transurethral laser ablation for the treatment of recurrent non‐muscle‐invasive bladder cancer. Laser irradiation was performed using LEONARDO DUAL (diode laser/980‐nm wavelength) and a 400‐μm BARE laser fiber. Pathological evaluation indicated low‐grade urothelial carcinoma, pTa. The patient was discharged on the second postoperative day with no perioperative or postoperative complications.ConclusionThis case suggests the potential of transurethral laser ablation for treating recurrent non‐muscle‐invasive bladder cancer.
Counselors’ theoretical orientations and competencies in counseling women
Authors: Han Eunae , Hoffman Lesa , Wood Susannah M. 
Published: October 2024

AbstractThe current study examined the relationship between theoretical orientations and competencies in counseling women among professional counselors. Counselors currently meeting clients were recruited from professional counseling organizations, and the responses from 265 counselors were analyzed using structural equation models. Results indicated that a stronger psychoanalytic orientation predicted lesser competencies in counseling women, while stronger humanistic, feminist, and multicultural orientations predicted higher competencies in counseling women. The study highlights the need for counselors to integrate theoretical orientations with social justice perspectives to better serve women.
Cybersecurity threats and preparedness: Implications for dental schools
Authors: Nalliah Romesh P. , Praveen Suvesha , Allareddy Trishul V. , Gajendrareddy Praveenkumar , Lee Min Kyeong , Oubaidin Maysaa , Allareddy Veerasathpurush 
Published: October 2024

AbstractBackgroundCybersecurity threats are a growing concern in healthcare, where digital systems now underpin patient care, financial management, and educational operations. A cybersecurity breach in a Dental school environment can have widespread consequences to the mission of the school—patient care, research, education and service. For dental school administrators, these risks highlight the necessity of robust cybersecurity measures. For student learners, the impact may include interruptions to their education. For patients, it could mean compromised personal data and reduced access to clinical care.Results & ConclusionWhile many sectors have responded to increasing cyber threats by enhancing their defenses, healthcare and dental schools, often lag in implementing necessary protections. This emphasizes the need for proactive measures, such as regular system audits, advanced encryption methods, and ongoing cybersecurity training for administrators and students alike, to mitigate future risks and safeguard institutional integrity.
Students’ perceptions of knowledge reinforcement on indirect prosthetic dental material choices by a translational approach
Authors: Smail Yasmine , Dursun Elisabeth , Ciers Jean‐Yves , Taleb Chakib , Nardari Catherine , Keosouvanh Nathalie , Heckly Lucie , Layan Pierre , Ceinos Romain , Jordan Laurence , Gouze Helene , Attal Jean‐Pierre , François Philippe 
Published: October 2024

AbstractPurpose/objectivesThe aim of this study is to evaluate students’ perceptions of the reinforcement of knowledge via innovative, case‐based, hands‐on learning regarding indirect prosthetic material choice.MethodsSix different clinical cases that represented common prosthetics were used in this simulation training. In each case, clinical pictures were associated with three‐dimensional (3D)‐printed replicates of final restorations and PolyJet polychromatic models with the goal of enabling students to deliberate and exchange ideas in small groups. After a debriefing session regarding the therapeutic potentialities of the first three cases alongside teachers, a lecture concerning prosthetic material choices was provided, and a zirconia crown was stained by each student to enable them to obtain a better understanding of the dental technician profession. Finally, the latter three cases were studied and analyzed in the same manner. The students’ perceived reinforcement of knowledge was recorded before and 1 month after the hands‐on simulation training experience, and their satisfaction was evaluated immediately thereafter on Likert scales. Students’ perceived reinforcement of knowledge was subjected to statistical evaluation.ResultsA high level of overall satisfaction was observed (4.60). All of the items pertaining to students’ satisfaction received scores >3. One month after this hands‐on approach, students’ confidence in their ability to choose a material on the basis of its mechanical, optical, and luting properties increased significantly (from 2.58 to 3.64; from 2.83 to 3.64; and from 2.72 to 3.58, respectively) (p < 0.05).ConclusionsThis innovative, hands‐on approach had a significant positive effect on students’ perceived reinforcement of knowledge.
Variability of three‐dimensional knee morphology cannot be effectively assessed using a coronal plane knee alignment classification in total knee arthroplasty patients
Authors: Jagota Ishaan , Twiggs Joshua , Miles Brad , Baré Jonathan V. 
Published: October 2024

AbstractPurposeOptimal reproduction of the native three‐dimensional (3D) alignment in total knee arthroplasty (TKA) influences outcomes; however, much of the modern TKA alignment research, such as the coronal plane alignment of the knee (CPAK), focuses only on coronal alignment. Tibial, femoral and tibiofemoral measurements on the axial and sagittal planes were evaluated for their relationship to the arithmetic hip‐knee‐ankle angle (aHKA) and joint‐line obliquity (JLO). These 3D anatomical measurements are also evaluated across CPAK groups.MethodsA retrospective analysis of the 360 Med Care computed tomography (CT) database was performed. Patient CT scans were segmented and landmarked. Linear regression analysis compared 12 axial and sagittal plane measurements (representing tibial, femoral and tibiofemoral rotation, tibial slope and femoral flexion) with both aHKA and JLO. Nonparametric tests assessed these anatomical measurements across the different CPAK groups, while Cohen's delta (d) determined the effect size.ResultsWith a sample size of 7450 osteoarthritic knees, significant but weak relationships (r < 0.30) were observed between all 12 anatomical measurements and both aHKA and JLO. Tibiofemoral rotations between Insall's axis and both the posterior condylar and the surgical transepicondylar axes demonstrated large effect sizes (d > 0.80). However, trivial to small effect sizes (d < 0.50) were broadly observed across the 12 axial and sagittal measurements, underscoring their limited clinical significance.ConclusionsWhile useful for describing coronal knee anatomy, CPAK phenotypes fail to differentiate tibial, femoral and tibiofemoral rotation, tibial slope or femoral flexion—crucial aspects of 3D surgical planning. Therefore, more comprehensive knee phenotyping solutions are required to guide individualised TKA alignment strategies.Level of EvidenceLevel II.
Short arm splints for wrist stabilization: A mechanical material test and cadaveric radiography study
Authors: Rasmussen Hans Christian , Bang Maya , Lilleøre Johanne Gade , Kipp Josephine Olsen , Lindgren Lars , Brüel Annemarie , Bue Mats , Mikkelsen Mads Kristian Duborg , Thomsen Jesper Skovhus , Stilling Maiken 
Published: October 2024

AbstractPurposeDocumentation of the wrist stabilizing effect and mechanical properties of common splinting materials is warranted to support evidence‐based condition‐specific recommendations for wrist immobilization. The objectives of this study were to assess the wrist stabilizing properties of volar and dorsal short‐arm splints made of four different materials and evaluate the mechanical properties of the splinting materials.MethodsDorsal and volar short arm splints made of plaster of Paris (PoP) (eight layers), Woodcast (2 mm, rigid vented), X‐lite (classic, two layers) or a 3D‐printed material (polypropylene) were sequentially mounted on 10 cadaveric arm specimens and fixed in a radiolucent fixture. This enabled the evaluation of maximum wrist flexion and extension relative under an orthogonal load of 42 N via radiographic images. In addition, a three‐point bending test was performed on ten sheet duplicates of each of the four splinting materials.ResultsWhen applied as a volar splint, PoP had the highest capability to resist wrist flexion and extension. However, when applied as a dorsal splint, Woodcast exhibited a lower wrist flexion and a similar wrist extension. The 3D‐printed splints—both volar and dorsal—showed the highest mean wrist flexion and extension. The mechanical properties of the Woodcast, X‐lite and 3D‐printed splinting materials were very similar. PoP exhibited distinct properties with a stiffness of 146 (95% confidence interval [CI]: 120–173) N/mm and a deflection at Fmax of 0.6 (95% CI: 0.5–0.7) mm compared to ≤7.7 (95% CI: 7.4–7.9) N/mm and ≥20 (95% CI: 18–22) mm for the other materials.ConclusionPoP displayed better wrist stabilizing properties and material stiffness than Woodcast, X‐lite and 3D‐printed polypropylene. When considering wrist stabilizing properties, PoP may still prove to be the preferred choice for wrist immobilization.Level of EvidenceNot applicable.
Characterization of Brain Abnormalities in Lactational Neurodevelopmental Poly I:C Rat Model of Schizophrenia and Depression Using Machine‐Learning and Quantitative MRI
Authors: Haker Rona , Helft Coral , Natali Shamir Emilya , Shahar Moni , Solomon Hadas , Omer Noam , Blumenfeld‐Katzir Tamar , Zlotzover Sharon , Piontkewitz Yael , Weiner Ina , Ben‐Eliezer Noam 
Published: October 2024

BackgroundA recent neurodevelopmental rat model, utilizing lactational exposure to polyriboinosinic‐polyribocytidilic acid (Poly I:C) leads to mimics of behavioral phenotypes resembling schizophrenia‐like symptoms in male offspring and depression‐like symptoms in female offspring.PurposeTo identify mechanisms of neuronal abnormalities in lactational Poly I:C offspring using quantitative MRI (qMRI) tools.Study TypeProspective.Animal ModelTwenty Poly I:C rats and 20 healthy control rats, age 130 postnatal day.Field Strength/Sequence7 T. Multiflip‐angle FLASH protocol for T1 mapping; multi‐echo spin‐echo T2‐mapping protocol; echo planar imaging protocol for diffusion tensor imaging.AssessmentNursing dams were injected with the viral mimic Poly I:C or saline (control group). In adulthood, quantitative maps of T1, T2, proton density, and five diffusion metrics were generated for the offsprings. Seven regions of interest (ROIs) were segmented, followed by extracting 10 quantitative features for each ROI.Statistical TestsRandom forest machine learning (ML) tool was employed to identify MRI markers of disease and classify Poly I:C rats from healthy controls based on quantitative features.ResultsPoly I:C rats were identified from controls with an accuracy of 82.5 ± 25.9% for females and 85.0 ± 24.0% for males. Poly I:C females exhibited differences mainly in diffusion‐derived parameters in the thalamus and the medial prefrontal cortex (MPFC), while males displayed changes primarily in diffusion‐derived parameters in the corpus callosum and MPFC.Data ConclusionqMRI shows potential for identifying sex‐specific brain abnormalities in the Poly I:C model of neurodevelopmental disorders.Level of EvidenceNATechnical EfficacyStage 2
Carvacrol enhances antioxidant activity and slows down cell wall metabolism by maintaining the energy level of ‘Guifei’ mango
Authors: Wang Xiaogang , Luo Donglan , Kou Xiaohong , Ye Shenjie , Li Jiangkuo , Ba Liangjie , Cao Sen 
Published: October 2024

AbstractBACKGROUNDPostharvest mango fruit are highly susceptible to rapid ripening, softening and senescence, greatly limiting their distribution. In this study, we evaluated the potential effects of carvacrol (0.06 g L−1) on mango (25 ± 1 °C) and the mechanisms by which it regulates antioxidant activity, energy and cell wall metabolism.RESULTSThe results showed that carvacrol treatment delayed the ‘Guifei’ mango color transformation (from green to yellow) and the decrease in firmness, titratable acidity, weight loss and soluble solids content, and suppressed the increase in relative conductivity, malondialdehyde content and reactive oxygen species (H2O2 and O2·–) as well as enhancing antioxidant activity. In addition, carvacrol treatment increased ascorbic acid and reduced glutathione levels, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase and dehydroascorbate reductase activities in mango. Meanwhile, energy level (adenosine triphosphate, adenosine diphosphate, adenosine monophosphate and energy charge) content and energy metabolizing enzyme activities (H+‐ATPase, Ca2+‐ATPase, succinate dehydrogenasepears and cytochrome C oxidase) were increased on carvacrol treatment, which resulted in the maintenance of higher energy levels. Finally, the application of carvacrol was effective in maintaining firmness and cell wall components by inhibiting the activities of polygalacturonase, cellulase, pectin methyl esterase and β‐galactosidase.CONCLUSIONThe current study demonstrates that carvacrol effectively delays the ripening and softening of mangoes by modulating energy metabolism and cell wall dynamics through the attenuation of oxidative stress. © 2024 Society of Chemical Industry.
High variability exists in 3D leg alignment analysis, but underlying principles that might lead to agreement on a universal framework could be identified: A systematic review
Authors: Veerman Quinten W. T. , ten Heggeler Romy M. , Tuijthof Gabriëlle J. M. , de Graaff Feike , Fluit René , Hoogeslag Roy A. G. 
Published: October 2024

AbstractPurposeTo (1) investigate the hypothesis that there is high variability in the reported methods to derive axes and joint orientations from three‐dimensional (3D) bone models to (a) perform 3D knee‐related leg alignment analysis and (b) define coordinate systems for the femur, tibia and leg and (2) identify underlying principles that might lead to agreement on a universal 3D leg alignment analysis framework.MethodsA systematic review of the literature between January 2006 and June 2024 was performed. Articles explicitly reporting methods to derive axes and joint orientations from CT‐based 3D bone models for alignment parameters and/or coordinate systems of the femur, tibia and leg were included. Study characteristics and reported methods were extracted and presented as a qualitative synthesis.ResultsA total of 93 studies were included. There was high variability in the reported methods to derive axes and joint orientations from 3D bone models. Nevertheless, the reported methods could be categorized into four groups, and several underlying principles of the four groups could be identified. Furthermore, the definitions of femoral and tibial coordinate systems were most frequently based on the mechanical axis (femoral, 13/19 [68%]; tibial, 13/26 [50%]) and a central medial‐lateral axis (femoral, 16/19 [84%]; tibial, 12/26 [46%]); no leg coordinate system was reported. Interestingly, of the included studies that reported on leg alignment parameters (76/93, 82%), only a minority reported expressing these in a complete coordinate system (25/76, 33%).ConclusionThere is high variability in 3D knee‐related leg alignment analysis. Therefore, universal 3D reference values for alignment parameters cannot yet be defined, and comparison of alignment parameter values between different studies is impossible. However, several underlying principles to the reported methods were identified, which could serve to reach more agreement on a future universal 3D framework for leg alignment analysis.Level of EvidenceLevel I (1).
Quantifying Health Utility of Age‐related Vocal Atrophy
Authors: de Leon Julio A. , Cvancara David J. , Carlson Abbey L. , Cheng Alice , Rizvi Zain H. , Giliberto J.P. , Sauder Cara , Bhatt Neel K. 
Published: October 2024

ObjectiveAge‐related vocal atrophy (ARVA) negatively impacts voice and quality of life (QOL). This study aims to determine utility‐based QOL in ARVA patients, correlate findings with traditional patient‐reported outcome measures (PROMs), and generate utility‐based inferences.MethodsForty ARVA patients were prospectively recruited from a tertiary care center. Health utility was measured using standard gamble, time trade‐off, and visual analog scale, assessing participants' current health states relative to defined comparison states (blindness/death). Traditional PROMs (Voice Handicap Index‐10 [VHI‐10] and Voice‐Related Quality of Life Scale [V‐RQOL]) were also collected. Descriptive and paired statistics were performed to determine health utility, and Pearson correlation assessed the association between PROMs and health utilities.ResultsMean health utility in ARVA was 0.84 ± 0.22, 0.88 ± 0.17, and 0.62 ± 0.25 using standard gamble, time trade‐off, and visual analog scale, respectively. There were positive correlations between V‐RQOL and time trade‐off (r = 0.66; p < 0.0001) as well as with standard gamble (r = 0.47; p = 0.002). Participants with ARVA reported no difference in health utility impact between their voice impairment and monocular blindness (Mean dif 10.9; 95% CI –1.6, 23.5; p = 0.101). Patients with ARVA were willing to part with an average 4.6 ± 6.1 years of life to restore normal voice.ConclusionsARVA patients view their voice impairment as a significant health decrement, comparable to monocular blindness. These findings underscore the substantial impact of ARVA on QOL and highlight the need for continued research and new therapies.Level of Evidence4 Laryngoscope, 135:1143–1147, 2025