bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping and Sequencing of a Significant Quantitative Trait Locus Affecting Resistance to Koi Herpesvirus in Common Carp
Authors: Palaiokostas Christos , Robledo Diego , Vesely Tomas , Prchal Martin , Pokorova Dagmar , Piackova Veronika , Pojezdal Lubomir , Kocour Martin , Houston Ross D 
Published: January 2019

AbstractCyprinids are the most highly produced group of fishes globally, with common carp being one of the most valuable species of the group. Koi herpesvirus (KHV) infections can result in high levels of mortality, causing major economic losses, and is listed as a notifiable disease by the World Organization for Animal Health. Selective breeding for host resistance has the potential to reduce morbidity and losses due to KHV. Therefore, improving knowledge about host resistance and methods of incorporating genomic data into breeding for resistance may contribute to a decrease in economic losses in carp farming. In the current study, a population of 1,425 carp juveniles, originating from a factorial cross between 40 sires and 20 dams was challenged with KHV. Mortalities and survivors were recorded and sampled for genotyping by sequencing using Restriction Site-Associated DNA sequencing (RADseq). Genome-wide association analyses were performed to investigate the genetic architecture of resistance to KHV. A genome-wide significant QTL affecting resistance to KHV was identified on linkage group 44, explaining approximately 7% of the additive genetic variance. Pooled whole genome resequencing of a subset of resistant (n = 60) and susceptible animals (n = 60) was performed to characterize QTL regions, including identification of putative candidate genes and functional annotation of associated polymorphisms. The TRIM25 gene was identified as a promising positional and functional candidate within the QTL region of LG 44, and a putative premature stop mutation in this gene was discovered.
Empirical Comparisons of Different Statistical Models To Identify and Validate Kernel Row Number-Associated Variants from Structured Multi-parent Mapping Populations of Maize
Authors: Yang Jinliang , Yeh Cheng-Ting “Eddy” , Ramamurthy Raghuprakash Kastoori , Qi Xinshuai , Fernando Rohan L , Dekkers Jack C M , Garrick Dorian J , Nettleton Dan , Schnable Patrick S 
Published: January 2019

Abstract
Advances in next generation sequencing technologies and statistical approaches enable genome-wide dissection of phenotypic traits via genome-wide association studies (GWAS). Although multiple statistical approaches for conducting GWAS are available, the power and cross-validation rates of many approaches have been mostly tested using simulated data. Empirical comparisons of single variant (SV) and multi-variant (MV) GWAS approaches have not been conducted to test if a single approach or a combination of SV and MV is effective, through identification and cross-validation of trait-associated loci. In this study, kernel row number (KRN) data were collected from a set of 6,230 entries derived from the Nested Association Mapping (NAM) population and related populations. Three different types of GWAS analyses were performed: 1) single-variant (SV), 2) stepwise regression (STR) and 3) a Bayesian-based multi-variant (BMV) model. Using SV, STR, and BMV models, 257, 300, and 442 KRN-associated variants (KAVs) were identified in the initial GWAS analyses. Of these, 231 KAVs were subjected to genetic validation using three unrelated populations that were not included in the initial GWAS. Genetic validation results suggest that the three GWAS approaches are complementary. Interestingly, KAVs in low recombination regions were more likely to exhibit associations in independent populations than KAVs in recombinationally active regions, probably as a consequence of linkage disequilibrium. The KAVs identified in this study have the potential to enhance our understanding of the genetic basis of ear development.
Geographical Features and Seroprevalence of Borrelia burgdorferi in Erzincan, Turkey
Authors: Cikman Aytekin , Aydin Merve , Gulhan Baris , Karakecili Faruk , Demirtas Levent , Arif Kesik Ozan 
Published: January 2019

Background: We aimed to determine the geographical features and seroprevalence of Borrelia burgdorferi in Erzincan, Turkey, which has a high tick population due to its geographical position and climatic conditions.
Methods: From January to December 2014, 368 people living in Erzincan, northeastern Turkey were enrolled. B. burgdorferi IgG antibodies were investigated in the collected serum samples using the ELISA method in 2015. Positive and borderline results were confirmed using the Western Blot (WB) method.
Results: Borrelia burgdorferi IgG positivity was found to be 4.1% by ELISA and 2.17% by WB. Of the seropositive people according to WB, 25% resided in areas within 2000m of rivers, 50% in areas with a slope of 0–5°, and 62.5% in areas with an altitude of lower than 1500 meters.
Conclusion: The seroprevalence of Lyme borreliosis was high in Erzincan, particularly among people engaged in animal husbandry in rural areas. In addition, the seroprevalence of Borrelia varied according to geographical features, increasing in areas with a lower slope and altitude.
Integrated Service Delivery and Health-Related Quality of Life of Individuals in Permanent Supportive Housing Who Were Formerly Chronically Homeless
Authors: Schick Vanessa , Wiginton Lindsey , Crouch Cathy , Haider Ali , Isbell Frances 
Published: January 2019

Objectives. To investigate the impact of an integrated care model on the health-related quality of life (HRQOL) of formerly chronically homeless individuals in permanent supportive housing.Methods. From 2014 to 2017, eligible individuals in Houston, Texas (n = 323), were placed in 1 of 2 permanent supportive housing service delivery models. Both models included coordinated care teams. In the intervention group, teams had a single plan of care with the partnering clinic. The 9-item Patient Health Questionnaire and 36-item Short Form Survey were administered at baseline and every 6 months for 30 months. We assessed intervention group emergency department use at 2 years. We evaluated change by using hierarchical linear growth models.Results. There was a significant and clinically meaningful increase in HRQOL in the intervention group, with the intervention group reporting improvement over the comparison group. Intervention group emergency department use decreased by 70% (no comparison group).Conclusions. Those in the intervention group with a single, coordinated plan of care reported significant and clinically meaningful increases in their HRQOL.Public Health Implications. Coordinated care models have potential to reduce societal costs and increase HRQOL, providing a financial and humanitarian justification for the continued investment in collaborative care in permanent supportive housing.
Type-3c Diabetes Mellitus, Diabetes of Exocrine Pancreas - An Update
Authors: Bhattamisra Subrat Kumar , Siang Tiew Chin , Rong Chieng Yi , Annan Naveenya Chetty , Sean Esther Ho Yung , Xi Lim Wen , Lyn Ong Siu , Shan Liew Hui , Choudhury Hira , Pandey Manisha , Gorain Bapi 
Published: January 2019

Background:The incidence of diabetes is increasing steeply; the number of diabetics has doubled over the past three decades. Surprisingly, the knowledge of type 3c diabetes mellitus (T3cDM) is still unclear to the researchers, scientist and medical practitioners, leading towards erroneous diagnosis, which is sometimes misdiagnosed as type 1 diabetes mellitus (T1DM), or more frequently type 2 diabetes mellitus (T2DM). This review is aimed to outline recent information on the etiology, pathophysiology, diagnostic procedures, and therapeutic management of T3cDM patients.Methods:The literature related to T3cDM was thoroughly searched from the public domains and reviewed extensively to construct this article. Further, existing literature related to the other forms of diabetes is reviewed for projecting the differences among the different forms of diabetes. Detailed and updated information related to epidemiological evidence, risk factors, symptoms, diagnosis, pathogenesis and management is structured in this review.Results:T3cDM is often misdiagnosed as T2DM due to the insufficient knowledge differentiating between T2DM and T3cDM. The pathogenesis of T3cDM is explained which is often linked to the history of chronic pancreatitis, pancreatic cancer. Inflammation, and fibrosis in pancreatic tissue lead to damage both endocrine and exocrine functions, thus leading to insulin/glucagon insufficiency and pancreatic enzyme deficiency.Conclusion:Future advancements should be accompanied by the establishment of a quick diagnostic tool through the understanding of potential biomarkers of the disease and newer treatments for better control of the diseased condition.
Circulating miRNA-21 and miRNA-23a Expression Signature as Potential Biomarkers for Early Detection of Non-Small-Cell Lung Cancer
Authors: Hetta Helal Fouad , Zahran Asmaa Mohammad , Shafik Engy A. , El-Mahdy Reham I. , Mohamed Nahed A. , Nabil Emad Eldin , Esmaeel Hend M. , Alkady Ola A. , Elkady Azza , Mohareb Dina A. , Hosni Amal , Mostafa Mohammed Mahmoud , Elkady Abeer 
Published: January 2019

Background and Aim:Lung Cancer (LC) is a major cancer killer worldwide, and 5-yr survival is extremely poor (≤15%), accentuating the need for more effective diagnostic and therapeutic strategies. Studies have shown cell-free microRNAs (miRNAs) circulating in the serum and plasma with specific expression in cancer, indicating the potential of using miRNAs as biomarkers for cancer diagnosis and therapy. This study aimed to identify differentially-expressed two miRNAs in the plasma of Non-Small Cell Lung Cancer (NSCLC) patients that might be a clinically useful tool for lung cancer early detection. miRNA-21 is one of the most abundant oncomirs. miRNA-23a functions as an oncogene in several human cancers, however, its clinical value has not been investigated in NSCLC.Materials and Methods:A case-control study was conducted in Assiut University Hospital, Egypt, from 2017 to 2018. Plasma samples were obtained from 45 NSCLC patients. The expression level of miR-21 and miRNA-23a was detected by qRT-PCR and compared to 40 healthy control subjects. The relation between both miRNAs and clinicopathological parameters was evaluated.Results:The expression level of miR-21 and miRNA-23a was significantly up-regulated (36.9 ± 18.7 vs. 1.12 ± 0.84 and 24.7 ± 19.09 vs. 1.16 ± 0.45) in NSCLC compared to matched controls (P<0.0001each). There was a significant difference in the level of plasma miRNA-21 and miRNA- 23a expression between the different grades of the disease (P = 0.032 and P = 0.001, respectively). The plasma miRNA-21 and miRNA-23a levels in the lung cancer patients with distant metastasis (n = 20) were significantly higher than those in the patients without metastasis (n = 25) (P<0.0001 each), the expression of miR-21 and miRNA-23a was significantly associated with tumor size (P = 0.001, P = 0.0001, respectively), but not significantly related to lymph node metastasis (P = 0.687 and 0.696, respectively). A positive correlation was observed between miRNA-21 and miRNA-23a (r = 0.784, P<0.01), There was no significant difference in the plasma miRNA-21 and miRNA-23a levels in the lung cancer patients with different histopathological types.Conclusion:miR-21 and miR-23a might play an oncogenic role in LC and is a poor prognostic factor. Switching off miRNA-21 and miRNA-23a may improve the treatment of LC. Our results must be verified by large-scale prospective studies with standardized methodology.
Recent Patents Applications in Red Biotechnology: A Mini-Review
Authors: Graciano Raíssa C.D. , Ribeiro Júlia A.T. , Macêdo Anderson K.S. , de S. Lavareda José Paulo , de Oliveira Pedro R. , Netto Jairo B. , Nogueira Laís M. , Machado Juliana M. , Camposda-Paz Mariana , Giunchetti Rodolfo C. , Galdino Alexsandro S. 
Published: January 2019

Background:
The different fields of biotechnology can be classified by colors, as a
"rainbow" methodology. In this sense, the red biotechnology, focused on the preservation of
health, has been outstanding in helping to solve this challenge through the provision of technologies,
including diagnostic kits, molecular diagnostics, vaccines, innovations in cancer research,
therapeutic antibodies and stem cells.

Objective:
The main goal of this work is to highlight the different areas within the red Biotechnology.
In this sense, we revised some patents regarding red biotechnology as examples to cover this
subject.

Methods:
A literature search of patents was performed from the followings Patents Database:
INPI, USPTO, Esp@cenet, WIPO and Google Patents.

Results:
Our analysis showed the following numbers from patents found: cancer research (8), diagnosis
kit (9), vaccines (8), stem cells (9) and therapeutic antibodies (5), where the United States
is the leader for most filled patents in Red Biotechnology.

Conclusion:
This mini-review has provided an update of some patents on Recent Patents in Red
Biotechnology. As far as we know, this is the first mini-review report on Red Biotechnology
based on patents.
Sublingual route for systemic drug delivery
Authors: Pawar Poonam P , Ghorpade Hemant S , Kokane Bhavana A. 
Published: January 2019

Drug delivery via the oral mucous membrane is considered to be a promising alternative to the oral route. Sublingual route is a rapid onset of action and better patient compliance than orally ingested tablets. Sublingual literally meaning is “under the tongue”, administrating substance via mouth in such a way that the substance is rapidly absorbed via blood vessels under tongue. The portion of drug absorbed through the sublingual blood vessels bypasses the hepatic first‐pass metabolic processes giving acceptable bioavailability. Sublingual technology is convenient for dosing in geriatric, pediatric and psychiatric patients with dysphagia. Sublingual drug delivery shows fast therapeutic action than orally ingested drugs with fewer side effects. This review highlights advantages, disadvantages, different sublingual Gland, sublingual formulation such as tablets, films drops, sprays etc, evaluation parameters.
Keywords: Sublingual delivery, dysphagia, sublingual gland, improved bioavailability, evaluations.
Formulation development and optimization of fast dissolving film containing carvedilol nanocrystals for improved bioavailability
Authors: Sharma Anju , Sriganesan P 
Published: January 2019

In this work, fast dissolving films (FDF) were prepared using nanocrystal formulations in order to optimise dissolution properties of lipophilic, poorly soluble drug Cavedilol. Drug nanocrystals are crystals with a size in the nanometer range, meaning that they are nanoparticles with a crystalline character. Carvedilol nanosuspensions were prepared using a high-pressure homogenizer, and then encapsulated in to films by solvent casting method using polymers such as maltodextrin and PVA in different concentrations. Propylene glycol used as a plasticizer. This study aimed to develop and evaluate the formulation of FDF containing Carvedilol nanocrystals for enhanced bioavailability and better compliance. The formulation of FDF was optimized by Box-Behnken Design (BBD) (design expert 11.03).In this design, 13 formulas were performed. One of the formula were suggested by design expert desirability = 1.
Keywords: Carvedilol, Nanocrystal, FDF, Box-bhenken optimization, in-vitro drug dissolution study,
UNITY AND IDENTITY: DECISIVE PRINCIPLES IN THE CATHOLIC THEOLOGY
Authors: VALENCIA PÉREZ Andrés 
Published: January 2019

This article is an analysis of the document Theology today: perspectives, principles and criteria published by the International Commission of the Roman Catholic Church. The foundations and principles of Catholic theology as unity and identity, require a permanent turn to the unique message of Christ, a message that we need to announce with courage and conviction. Therefore, it is legitimate to speak about the need for a certain unity of theology. Thus, we must bear in mind that the concept of unity needs to be carefully understood so as not to be confused with uniformity or with an individual style. The unity of theology and the whole Church, as professed in the Creed, must be closely linked to the idea of catholicity, as well as to holiness and apostolicity. Uniqueness and identity come from Christ, Savior of the world. The theology of unity and identity that wants to be Catholic must participate in the catholicity and in the unity of the Church, which ultimately is based on the trinitarian unity of God himself. Theology is catholic in the richness of the plurality of its expressions, protagonists, ideas and contexts and, therefore it is born from the attentive listening of the Word of God, it is incardinated in the communion of the Church and it is oriented to the service of the truth.
Treatment of a collapsible soil using a bentonite–cement mixture
Authors: Bellil Soumia , Abbeche Khelifa , Bahloul Ouassila 
Published: January 2019

Abstract
The study of collapsible soils that are generally encountered in arid and semi-arid regions remains a major issue for geotechnical engineers. This experimental study, carried out on soils reconstituted in the laboratory, aims firstly to present a method of reducing the collapse potential to an acceptable level by treating them with different levels of bentonite–cement mixture while maintaining the water content and degree of compactness, thus reducing eventual risks for the structures implanted on these soils. Furthermore, a microscopic study using scanning electron microscopy was carried out to explore the microstructure of the soil in order to have an idea of the phenomena before and after treatment. The results show that treatment with a bentonite–cement mixture improves the geotechnical and mechanical characteristics, modifies the chemical composition of the soil, reduces the collapse potential and the consistency limits. The microstructural study and the X-ray energy dispersive spectroscopy analysis clearly illustrate an association of elementary particles in the soil aggregates, whereby the arrangement of these aggregates leads to the formation of a dense and stable material.
Stress states caused in chamber of reinforced concrete grain silo by non-centric emptying on large eccentricities
Authors: Anna Prusiel Jolanta , Gierej Krzysztof 
Published: January 2019

Abstract
The article presents the analysis of complex stress states in the concrete structure of grain silos, caused by non-centric emptying. The authors present a combination of loads from the pressure of bulk solid on the silo chamber according to Eurocode 1, Part 4 [11], which should be taken into account when emptying on large eccentricities in action assessment class 3 (AAC3) silos. For the example of a cylindrical wheat silo with a height of 25 m and a diameter of 10 m, the researchers carried out an analysis regarding the impact of the size of the eccentric discharge outlet on the distributions of forces and bending moments in a reinforced concrete wall.
PENCARIAN LOKASI RUMAH SAKIT DI KABUPATEN PROBOLINGGO DENGAN METODA DJIKSTRA BERBASIS SPASIAL
Authors: Sarwo Setyohadi Dwi Putro , Rizaldi Taufiq 
Published: January 2019

Perkembangan ilmu dibidang teknologi komputer telah membawa manfaat yang sangat besar bagi penyebaran informasi. Selain sebagai sumber informasi, komputer juga dimanfaatkan sebagai sarana penentu letak geografis pada suatu wilayah. BanyakÂ sekaliÂ aplikasiâ€“aplikasi yangÂ dapatÂ ditanganiÂ olehÂ sistem informasi geografis, Di antaranya adalah padaÂ bidangÂ rumah sakit yang dapatÂ dilakukanÂ denganÂ bantuan SIGÂ adalah informasi letak dan jalur untuk menuju lokasi rumah sakit. Pencarian jalur sangat diperlukan bagi pengguna jalan yang tidak tahu jalan mana yang akan dilalui agar sampai ke tempat tujuannya dalam suatu kota. Penghematan waktu dan biaya menjadi faktor lain yang mengharuskan pengguna jalan mencari suatu jalur yang terpendek agar lebih cepat sampai ke tempat tujuan. Oleh karena hal itulah maka pencarian jalur terpendek menjadi suatu permasalahan yang patut untuk diselesaikan secara komputerisasi dengan kecerdasan buatan. Dari permasalahan tersebut, Dirancang suatu sistem yang dapat memetakan tempat rumah sakit di kabupaten Probolinggo. Sehingga dapat membantu masyarakat dalam mencari letak dan kecamatan terdekat menuju lokasi rumah sakit. Pemetaan dan informasi rumah sakit ini akan diangkat pada penlitian yang berjudul " PENCARIAN LOKASI RUMAH SAKIT DI KABUPATEN PROBOLINGGO DENGAN METODA DJIKSTRA BERBASIS SPASIAL".
RANCIÃˆRE AND THE RECUPERATION OF POLITICS
Authors: Olivier Bert 
Published: January 2019

Â In the work of Jacques RanciÃ¨re one encounters a welcome and uncompromising return to the question of the political, or politics proper, as opposed to politics in the ordinary sense of the word. For RanciÃ¨re, the political is something irreducible, where the fundamental equality of all human subjects manifests itself, while customary politics is the perversion of the political in as far as it covers up this equality and institutes in its place a hierarchical arrangement of the polis. Hence RanciÃ¨reâ€™s claim that customary politics is the work of what he calls the â€œpoliceâ€ (not with the usual meaning), which here represents the agency that parcels out the polis according to the interests of those who have a â€œpartâ€ in it. RanciÃ¨reâ€™s concern, however, is for the part of the de-mos, or those â€œwith no partâ€, who are at once excluded from politics and immanent to it as its constant other, or shadow. This paper explores the implications of RanciÃ¨reâ€™s radicalisation of the notion of the â€œpoliticalâ€ â€“ or â€œpoliticsâ€ in the sense of the democratic pursuit of equality â€“ for the hierarchical, consensual realm of (pseudo-) politics under the â€œpoliceâ€, and for the prospects of democracy, especially considering the role of what RanciÃ¨re calls â€œdissensusâ€.
Helical Structures of Cyclopentene-based α,α-Disubstituted α-Amino Acid Homopeptides
Authors: Tanaka Masakazu , Yakabi Haruka , Nakatani Haruki , Ueda Atsushi , Doi Mitsunobu , Oba Makoto 
Published: January 2019

The cyclopentene-based α,α-disubstituted α-amino acid Ac5c= and its homopeptides, up to nonapeptides, were synthesized. The side-chain cyclopentene was expected to become symmetric, the Cα-carbon to be puckered, and other Cβ, Cβ', Cγ, Cγ'-carbons to be coplanar. As expected, side-chain cyclopentene conformations became symmetric and Cα-carbons were puckered. Conformational studies using FT-IR absorption, 1H NMR spectra, and X-ray crystallographic analyses revealed that Ac5c= homopeptides did not form a planar conformation, but assumed a 310-helical structure, similar to cyclopentane-based α,α-disubstituted α-amino acid homopeptides.
Dynamic recrystallization of quartz in veins in sandstones of metagenesis zone (Kular district, Yakutia)
Authors: Sustavov O. A. 
Published: January 2019

Bulging recrystallization (BR) has been considered in the deformed quartz veins in the lower Triassic sandstones of the metagenesis zone in the North-Western part of the Kular district, Yakutia. BR is characterized by the formation of sutured boundaries of individuals during the crystalloplastic deformation of quarz. To these boundaries, the recrystallization grains are confined, the size of which (in this case, 0,01—0,02 mm) corresponds to the size of the teeth at the boundaries of individuals. Such fine recrystallization grains can fix with their formation the peak crustal stresses arising up during deformation of the continental crust near the brittle-plastic transition. Inside the individuals, BR is accompanied by recrystallization along the microshears and deformation bands with the leading role of progressive rotation of subgrains. BR in vein quartz occurs during the formation of the pressure solution cleavage in the host sandstones, and therefore the combination of the pressure solution cleavage in the sandstones of the metagenesis zone and BR in vein quartz can be considered as structural paragenesis.