bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of Recombinant Tissue Plasminogen Activator Complications in Treatment of patients with ischemic stroke visiting Vali-e-Asr hospital in Birjand, 2016-2017
Authors: Riasi Hamid Reza , Zarei Elham , Salehi Forod , Sayehmiri Fatemeh 
Published: August 2019

Abstract
Background: Current treatment options for the sake of treating acute ischemic stroke include recombinant tissue plasminogen activator or dual anti platelet therapies. This study aims to evaluate the complications of recombinant tissue plasminogen activator in treatment of patients with ischemic stroke who were admitted in Vali-e-Asr hospital in Birjand, 2016-2017. Method: This descriptive analytic study was performed on patients with acute ischemic stroke who were admitted in neurology ward of Vali-e-Asr hospital in Birjand from 2016 to 2017. A total of 127 patients participated in this study. The data about complications of treatment were collected by questionnaires and entered into SPSS 21. Then, data were analyzed by Chi-square or Fisher exact test at a significant level of p≤ 0.5. Results: A total of 127 subjects received treatment for ischemic stroke. Thirty-one (24.4%) patients have been treated with recombinant tissue plasminogen activator and ninety-six (75.6%) have been treated conventionally with dual antiplatelet. These two groups were matched in terms of age and sex. The history of hypertension in the recombinant tissue plasminogen activator group and the conventional treatments were 32.3% and 67.7%, respectively (p=0.03). 99% of patients in the antiplatelet treatment group (N=96) and 96.8% of patients in the recombinant tissue plasminogen activator group (N=31) have been discharged and one death was occurred in each group (p=0.4). Regarding the incidence of recombinant tissue plasminogen activator complications, IVH was reported in two patients (6.5%, p = 0.06) Conclusion: The incidence of mortality was the same in two groups. Also, complications were only reported in two patients in the recombinant tissue plasminogen activator group (both intraventricular hemorrhage) and the difference was not statistically significant. Researchers recommend that more clinical trials must be conducted. If it is approved, the findings of the current studies will be widely taken into consideration for acute stroke treatment.
Reinterpretation, reclassification, and its downstream effects: Challenges for clinical laboratory geneticists
Authors: Mecky Julia el , Johansson Lennart , Plantinga Mirjam , Fenwick Angela , Lucassen Anneke , Dijkhuizen Trijnie , Hout Annemieke van der , Lyle Kate , Langen Irene van 
Published: August 2019

Abstract
Background. In recent years, the amount of genomic data produced in clinical genetics services has increased significantly due to the advent of next-generation sequencing. This influx of genomic information leads to continuous changes in knowledge on how genetic variants relate to hereditary disease. These changes can have important consequences for patients who have had genetic testing in the past, as new information may affect their clinical management. When and how patients should be recontacted after new genetic information becomes available has been investigated extensively. However, the issue of how to handle the changing nature of genetic information is significantly underexplored in a laboratory setting, despite it being the first stage at which changes in genetic data are identified and managed. Methods. The authors organized a 7-day online focus group discussion in which all Dutch molecular genetics diagnostic laboratories were represented. Results. Laboratories in our study reinterpret genetic variants reactively, e.g. at the request of a clinician or following identification of a previously classified variant in a new patient. Participants currently deemed active, periodic reinterpretation to be unfeasible and opinions differed on whether it is desirable, particularly in regard to patient autonomy and the main responsibilities of the laboratory. The efficacy of reinterpretation was questioned in the presence of other strategies, such as reanalysis and resequencing of DNA. Despite absence of formal policy regarding when to issue a new report for clinicians due to reclassified genetic data, participants indicated similar practice across all laboratories. However, practice differed significantly between laboratory geneticists regarding the reporting of VUS reclassifications. Conclusion. Based on the results, the authors formulated five challenges needing to be addressed in future laboratory guidelines: 1. Should active reinterpretation of variants be conducted by the laboratory as a routine practice? 2. How does reinterpretation initiated by the laboratory relate to patient expectations and consent? 3. When should reinterpreted data be considered clinically significant and communicated from laboratory to clinician? 4. Should reinterpretation, reanalysis or a new test be conducted? 5. How are reclassifications perceived and how might this affect laboratory practice?
Incidence of hypoxemia in a post-anaesthesia care unit and relevant risk factors: a retrospective study of 14604 patients with general anaesthesia
Authors: Tang Shuai , Zhang Liangyan , Han Wei , Xue Yang , Tian Yi , Zhang Xiuhua , Huang Yuguang 
Published: August 2019

Abstract
Background This article is aim to investigate the incidence and risk factors for postoperative hypoxemia in a post-anaesthesia care unit (PACU). Methods The retrospective cohort assessed 14604 postoperative patients who were admitted to PACU between January 2015 and December 2015. A pulse oximeter was used to monitor and record pulse oxygen saturation (SpO2) every 5 minutes. Clinical data were collected for all these patients, and the incidence of and risk factors for postoperative hypoxemia were analysed. Results The total incidence of hypoxemia was 21.83% (SpO2 ≤ 95%) and 2.79% (SpO2 ≤ 90%). Multiple regression analysis indicated that the risk factors were age ≥50-year old, body mass index (BMI) ≥25kg/m2, American Society of Anaesthesiologists (ASA) II and III, limb surgery, and thoracic surgery. Conclusions Therefore, hypoxemia was common in postoperative patients in the PACU. Age, BMI, ASA classification, and surgical site are associated with postoperative hypoxemia. More attention should be paid to these patients to prevent hypoxemia in the PACU.
PROtective Ventilation with a low versus high Inspiratory Oxygen fraction(PROVIO) and its effects on postoperative pulmonary complications:protocol for a randomized controlled trial
Authors: Li Xue-Fei , Jiang Dan , Jiang Yu-Lian , Yu Hong , Jiang Jia-Li , He Lei-Lei , Yang Xiao-Yun , Yu Hai 
Published: August 2019

Abstract
Background: Postoperative pulmonary complications (PPCs) is the most common perioperative complication following surgical site infection (SSI), which prolongs the hospital stay and increases health care cost. Lung-protective ventilation strategy is considered better practice in abdominal surgery to prevent PPCs. However, the role of inspiratory oxygen fraction (FiO₂) in the strategy remains disputable. Previous trials have focused on reducing SSI by increasing inhaled oxygen concentration but higher FiO₂ (80%) was found to be associated with a greater incidence of atelectasis and mortality in recent researches. The trial aims at evaluating the effect of different FiO₂ added to lung-protective ventilation strategy on the incidence of PPCs during general anesthesia for abdominal surgery.
Methods: PROtective Ventilation with a low versus high Inspiratory Oxygen fraction trial（PROVIO）is a single-center, prospective, randomized, controlled trial planning to recruit 252 patients undergoing abdominal surgery lasting for at least 2 hours. The patients will be randomly assigned to (1) a low FiO₂ (30% FiO₂) group and (2) a high FiO₂ (80% FiO₂) group in lung-protective ventilation strategy. The primary outcome of the study is the occurrence of PPCs within the postoperative 7 days. Secondary outcomes include the severity grade of PPCs, the occurrence of postoperative extrapulmonary complications and all-cause mortality within the postoperative 7 and 30 days.
Discussion: PROVIO trial assesses the effect of low versus high FiO₂ added to lung-protective ventilation strategy on PPCs for abdominal surgery patients and the results will provide practical approaches to intraoperative oxygen management.
Trial registration number: Registered at www.ChiCTR.org.cn on 13 February 2018 with identifier no. ChiCTR18 00014901. Keywords: Postoperative pulmonary complications, Lung-protective ventilation, Fraction of inspired oxygen, Abdominal surgery.
The prevalence of HIV among MSM in China: a large-scale systematic analysis
Authors: Dong Mengjie , Peng Bin , Liu Zhen-Feng , Ye Qian-ni , Liu Hao , Lu Xi-Li , Zhang Bo , Chen Jia-Jia 
Published: August 2019

Abstract
Background：The prevalence of HIV among men who have sex with men (MSM) has become a significant public health challenge. The aim was to comprehensively estimate the national prevalence of HIV among MSM and its time trends through a large-scale systematic analysis.
Methods：Systematic search of Cochrane Library, PubMed, EMBASE, CNKI, VIP, and Wanfang Data databases without language restriction for studies on the prevalence of HIV among MSM published before Dec.31, 2018. Studies were eligible for inclusion if they were published in the peer-reviewed literature and used validated assessment methods to assess the prevalence of HIV among MSM. Estimates were pooled using random-effects analysis.
Results：Data were extracted from 355 cross-sectional studies (571,328 individuals) covered 59 cities from 30 provinces and municipalities of China. The overall national prevalence of HIV among MSM from 2001 to 2015 was estimated to be 5.7% (95% CI: 5.4–6.1%), with high between-study heterogeneity (I2 = 98.0%, P < 0.001). Our study showed an increased tendency in the HIV prevalence as time progressed by meta-regression analysis (I2= 95.9%, P<0.0001). HIV prevalence was the highest in those aged 50 years and older with HIV prevalence of 19.3% (95%CI: 13.1-27.4%, N=13). HIV was more prevalent in the illiterate population (16.8%), than in those who had received an education. Although the internet was a major venue for Chinese MSM seeking male sex partners (35.6%, 95%CI: 32.3%-39.9%, N=101), seeking MSM in bathhouses/saunas had the highest associated prevalence of HIV (13.4%, 95%CI: 10.3%-17.1%, N=22). The HIV prevalence among MSM varied by location: compared with other regions in China, HIV was highly prevalent among MSM in the southwest (10.7%, 95%CI: 9.3%-12.2%, N=91). Compared to participants who sometimes or always used condoms, participants who had never used a condom in the past 6 months had a higher risk of HIV infection, with odds ratios of 0.1 (95%CI: 0.08-0.14).
Conclusions：Our analysis provided reliable estimates of China’s HIV burden among MSM, which appears to present an increasing national public health challenge. Effective government responses are needed to address this challenge and include the implementation of HIV prevention.
Using a Hepatitis B surveillance system evaluation in Fujian, Hainan, and Gansu Provinces to improve data quality and assess program effectiveness, China, 2015
Authors: Zheng Hui , Millman Alexander J. , Rainey Jeanette J. , Wang Fuzhen , Zhang Rui , Chen Hong , Yin Zundong , Wang Huaqing , zhang Guomin 
Published: August 2019

Abstract
Background: Monitoring hepatitis B surveillance data is important for evaluating progress towards global hepatitis B elimination goals. Accurate classification of acute and chronic hepatitis infections is essential for assessing program effectiveness.
Methods: We evaluated hepatitis B case-reporting at six hospitals in Fujian, Hainan and Gansu provinces in 2015 to assess the accuracy of case classification. We linked National Notifiable Disease Reporting System (NNDRS) HBV case-reports with hospital information systems and extracted information on age, gender, admission ward and viral hepatitis diagnosis from medical records. To assess accuracy, we compared NNDRS reported case-classifications with the national HBV case definitions. Multivariable logistic regression was used to identify factors associated with misclassification.
Results: Of the 1,420 HBV cases reported to NNDRS, 23 (6.5%) of the 352 acute reports and 648 (60.7%) of the 1,068 chronic reports were correctly classified. Of the remaining, 318 (22.4%) were misclassified and 431 (30.4%) could not be classified due to the lack of supporting information. Based on the multivariable analysis, HBV cases reported from Hainan (aOR=1.8; 95% CI: 1.3-2.4) and Gansu (aOR=12.7; 95% CI: 7.7-20.1) along with reports from grade 2 hospitals (aOR=1.6; 95% CI:1.2-2.2) and those from non-HBV related departments (aOR=5.3; 95% CI: 4.1-7.0) were independently associated with being ‘misclassified’ in NNDRS.
Conclusions: We identified discrepancies in the accuracy of HBV case-reporting in the project hospitals. Onsite training on the use of anti-HBc IgM testing as well as on HBV case definitions and reporting procedures are needed to accurately assess program effectiveness and ensure case-patients are referred to appropriate treatment and care. Routine surveillance evaluations such as this can be useful for improving data quality and monitoring program effectiveness.
Benchmarking Variant Identification Tools for Plant Diversity Discovery
Authors: Wu Xing , Heffelfinger Christopher , Zhao Hongyu , Dellaporta Stephen L. 
Published: August 2019

Abstract
Background The ability to accurately and comprehensively identify genomic variations is critical for plant studies utilizing high-throughput sequencing. Most bioinformatics tools for processing next-generation sequencing data were originally developed and tested in human studies, raising questions as to their efficacy for plant research. A detailed evaluation of the entire variant calling pipeline, including alignment, variant calling, variant filtering, and imputation was performed on different programs using both simulated and real plant genomic datasets. Results A comparison of SOAP2, Bowtie2, and BWA-MEM found that BWA-MEM was consistently able to align the most reads with high accuracy, whereas Bowtie2 had the highest overall accuracy. Comparative results of GATK HaplotypCaller versus SAMtools mpileup indicated that the choice of variant caller affected precision and recall differentially depending on the levels of diversity, sequence coverage and genome complexity. A cross-reference experiment of S. lycopersicum and S. pennellii reference genomes revealed the inadequacy of single reference genome for variant discovery that includes distantly-related plant individuals. Machine-learning-based variant filtering strategy outperformed the traditional hard-cutoff strategy resulting in higher number of true positive variants and fewer false positive variants. A 2-step imputation method, which utilized a set of high-confidence SNPs as the reference panel, showed up to 60% higher accuracy than direct LD-based imputation. Conclusions Programs in the variant discovery pipeline have different performance on plant genomic dataset. Choice of the programs is subjected to the goal of the study and available resources. This study serves as an important guiding information for plant biologists utilizing next-generation sequencing data for diversity characterization and crop improvement.
Transcriptome changes reveal the genetic mechanisms of the reproductive plasticity of workers in lower termites
Authors: Ye Chenxu , Rasheed Humaira , Ran Yuehua , Yang Xiaojuan , Xing Lianxi , Su Xiaohong 
Published: August 2019

Abstract
Background: The reproductive plasticity of termite workers provides colonies with tremendous flexibility to respond to environmental changes, which is the basis for evolutionary and ecological success. Although it is known that all colony members share the same genetic background and that differences in castes are caused by differences in gene expression, the pattern of the specific expression of genes involved in the differentiation of workers into reproductives remains unclear. In this study, the isolated workers of Reticulitermes labralis developed into reproductives, and then comparative transcriptomes were used for the first time to reveal the molecular mechanisms underlying the reproductive plasticity of workers.
Results: We identified 38,070 differentially expressed genes and found a pattern of gene expression involved in the differentiation of the workers into reproductives. 12,543 genes were specifically upregulated in the isolated workers. Twenty-five signal transduction pathways classified into environmental information processing were related to the differentiation of workers into reproductives. Ras functions as a signalling switch regulates the reproductive plasticity of workers.The catalase gene which is related to longevity was up-regulated in reproductives.
Conclusion: We demonstrate that workers leaving the natal colony can induce the expression of stage-specific genes in the workers, which leads to the differentiation of workers into reproductives and suggests that the signal transduction along the Ras-MAPK pathway crucially controls the reproductive plasticity of the workers. This study also provides an important model for revealing the molecular mechanism of longevity changes.
Investigation of the kieserite–szomolnokite solid-solution series, (Mg,Fe)SO4·H2O, with relevance to Mars: Crystal chemistry, FTIR, and Raman spectroscopy under ambient and martian temperature conditions
Authors: Talla Dominik , Wildner Manfred 
Published: August 2019

AbstractThe investigation of hydrous sulfate deposits and sulfate-cemented soils on the surface of Mars is one of the important topics in the recent scientific endeavor to retrieve detailed knowledge about the planetary water budget and surface weathering processes on our neighbor planet. Orbital visible/near-IR spectra of the surface of Mars indicate kieserite, MgSO4·H2O, as a dominant sulfate species at lower latitudes. However, given the Fe-rich composition of the martian surface, it is very probable that its actual composition lies at an intermediate value along the solid-solution series between the kieserite and szomolnokite (FeSO4·H2O) end-members. Despite the known existence of significant lattice parameter changes and spectral band position shifts between the two pure end-members, no detailed crystal chemical and spectroscopic investigation along the entire kieserite–szomolnokite solid solution range has been done yet.The present work proves for the first time the existence of a continuous kieserite–szomolnokite solid-solution series and provides detailed insight into the changes in lattice parameters, structural details, and positions of prominent bands in FTIR (5200–400 cm–1) and Raman (4000–100 cm–1) spectra in synthetic samples as the Fe/Mg ratio progresses, at both ambient as well as Mars-relevant lower temperatures. Additionally, an UV-Vis-NIR (29 000–3500 cm–1) crystal field spectrum of szomolnokite is presented to elucidate the influence of Fe2+-related bands on the overtone- and combination mode region.The kieserite–szomolnokite solid-solution series established in this work shows Vegard-type behavior, i.e., lattice parameters as well as spectral band positions change along linear trends. The detailed knowledge of these trends enables semi-quantitative estimations of the Fe/Mg ratio that can be applied to interpret martian monohydrated sulfates in data from remote sensing missions on a global scale as well as from in situ rover measurements. Given the knowledge of the surface temperature during spectral measurements, the established temperature behavior allows quantitative conclusions concerning the Fe/Mg ratio. Our understanding of the kieserite–szomolnokite solid-solution series will be well applicable to the Mars 2020 and ExoMars 2020 rover missions that will focus on near IR (0.9 to 3.5 μm) and, for the first time on Mars, Raman spectroscopy.
Change of crackling noise in granite by thermal damage: Monitoring nuclear waste deposits
Authors: Xie Kainan , Jiang Xiang , Jiang Deyi , Xiao Yang , Chen Shiwan , Dahmen Karin A. , Vives Eduard , Planes Antoni , Salje Ekhard K.H. 
Published: August 2019

Abstract
High-sensitivity detection of acoustic emission from granite under uniaxial stress, together with advanced statistical analysis, shows changing collapse mechanisms when a sample is pre-heated. Massive microstructural changes occur at temperatures >500 °C while low-temperature (<<500 °C) treatment leads to scale invariant crackling noise with a mixed fix-point behavior. After treatment at higher temperatures, the collapse occurs via acoustic signals that show energy distributions with systematic deviations from the Gutenberg-Richter law while the Omori's and Båth's laws are not influenced by the thermal treatment. The granite samples stem from the site in the Beishan mountains where a new burial site for nuclear waste will be constructed. According to the 13th Five-Year Plan of the P.R. China, Chinese nuclear power installed capacity will reach 58 million kilowatts in 2020 and produce about 3200 tons of high-level nuclear waste every year. Monitoring the stability of the host rock at high temperatures becomes hence a key issue. Our analysis can serve as a blueprint for a protocol for continuous monitoring of the burial site.
Listeria sp., en cantinas de almacenamiento de leche cruda de vaca en Tunja – Boyacá
Authors: Urbano-Cáceres Eliana , Aguilera-Becerra Astrid , Jaimes-Bernal Claudia , Pulido-Medellín Martín 
Published: August 2019

Objetivo. Determinar la relación entre el tipo de detergente, el tiempo de uso de la cantina y el tipo de agua utilizada para lavado de las cantinas y la presencia de Listeria sp. en muestras recolectadas a partir de cantinas de almacenamiento de leche cruda de vaca, en fincas del municipio de Tunja-Boyacá-Colombia. Materiales y métodos. Se recolectaron 293 muestras mediante muestreo no probabilístico a conveniencia, en un periodo de nueve meses. El aislamiento de Listeria se realizó por métodos microbiológicos y la identificación de especies utilizando pruebas bioquímicas. Se aplicó un cuestionario para evaluar los factores asociados (tipo de detergente, tipo de agua y tiempo de uso de las cantinas). Resultados. La prevalencia de L. monocytogenes fue de 2.7% (n=8). No se encontró asociación estadísticamente significativa entre las variables relacionadas con la limpieza de las cantinas y la presencia de L. monocytogenes. Conclusiones. Se demostró la existencia de L. monocytogenes en leche cruda, siendo la prevalencia encontrada inferior a las reportadas en estudios nacionales e internacionales. Se evidencia la circulación de especies de Listeria sp en la cadena productiva láctea del departamento de Boyacá. Por otra parte, es el primer panorama de L. monocytogenes y L. ivanovii mostrando la necesidad de la implementación de medidas de control en la industria láctea.