bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pursuing the question of reflexivity in psychotherapy and qualitative methods: The contributions of David L. Rennie
Authors: Levitt Heidi M. , Lu Ethan C. , Pomerville Andrew , Surace Francisco I. 
Published: November 2018

AbstractBackgroundThis article documents the intellectual contributions of the psychologist David Rennie to the fields of psychotherapy research, qualitative methods and humanistic psychology by tracing the central position of reflexivity across these bodies of work. This work had a profound influence on his field and led to his viewing the fostering of clients’ reflexivity as the central task of psychotherapy.AimsIt follows his path as a researcher, beginning with his advancement of grounded theory methods and their application within psychotherapy research. His development of a humanistic experiential person‐centred approach to psychotherapy is described as an extension of this valuing of reflexivity and the recognition of the role of clients’ agency within sessions. In addition, the paper reviews the innovative methodological research that led him to propose a model of inferential processes that underlies qualitative research methods and that is rooted in the process of embodied reflexivity. His contextualising qualitative methods within a form of methodical hermeneutics permitted a unifying framework for understanding the logic of qualitative methods.
Long non‐coding RNA RUNX1‐IT1 plays a tumour‐suppressive role in colorectal cancer by inhibiting cell proliferation and migration
Authors: Shi Jinxin , Zhong Xi , Song Yongxi , Wu Zhonghua , Gao Peng , Zhao Junhua , Sun Jingxu , Wang Jiajun , Liu Jingjing , Wang Zhenning 
Published: November 2018

Long non‐coding RNAs (lncRNAs) have been demonstrated to be involved in the progression of various cancers. In this study, we aim to investigate the role of lncRNA RUNX1‐IT1 in the development of colorectal cancer (CRC). The expression levels of lncRNA RUNX1‐IT1 were measured using quantitative real‐time Polymerase Chain Reaction(qRT‐PCR). CCK8 proliferation assay, transwell assay, and flow cytometry were performed to evaluate the effect of lncRNA RUNX1‐IT1 on CRC cell proliferation, migration, and apoptosis. The proliferation markers (PCNA, Ki67), apoptosis markers (cleaved‐PARP, cleaved‐caspase3), and MMP9 are detected by western blotting. Significant down regulation of lncRNA RUNX1‐IT1 was measured in CRC tissues and three CRC cell lines (HCT116, HT29, and RKO) compared with paired nontumorous adjacent tissues (P < 0.01) or the normal colonic epithelial cell line FHC (P < 0.05), respectively. Moreover, the proliferative and migration potential of CRC cells were inhibited by overexpressing lncRNA RUNX1‐IT1, which could be obviously improved by knocking down lncRNA RUNX1‐IT1. The protein levels of PCNA, Ki67, and MMP9 were upregulated by overexpressing lncRNA RUNX1‐IT1 and down regulated in si‐RUNX1‐IT1 cells. Besides, lncRNA RUNX1‐IT1 could also promote the apoptosis of CRC cells. In conclusion, lncRNA RUNX1‐IT1 is downregulated in CRC and plays a tumour‐suppressive role due to the regulatory of cell proliferation, migration, and apoptosis.Significance of the studyWe demonstrated that lncRNA RUNX1‐IT1 was down regulated both in CRC tissues and cell lines. Besides, lncRNA RUNX1‐IT1 could serve as a potential diagnostic biomarker and play a tumour‐suppressive role owing to its good diagnostic efficacy and inhibition of CRC cell proliferation and migration.
Novel Dextran‐Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate–Protein Interactions of Gal‐3
Authors: Rahkila Jani , Ekholm Filip S. , Ardá Ana , Delgado Sandra , Savolainen Johannes , Jiménez‐Barbero Jesús , Leino Reko 
Published: November 2018

AbstractThe quest for novel natural‐like biomolecular probes that can be used to gain information on biological recognition events is of topical interest to several scientific areas. In particular, the recognition of carbohydrates by proteins modulates a number of important biological processes. These molecular recognition events are, however, difficult to study by the use of naturally occurring oligosaccharides and polysaccharides owing to their intrinsic structural heterogeneity and to the many technical difficulties encountered during the isolation of sufficient quantities of pure material for detailed structural and biological studies. Therefore, the construction of homogenous biomolecular probes that can mimic both the biophysical properties of polysaccharide backbones and the properties of bioactive oligosaccharide fragments are highly sought after. Herein, synthetic methodology for the construction of well‐defined bioconjugates consisting of biologically relevant disaccharide fragments grafted onto a dextran backbone is presented, and a preliminary NMR spectroscopy study of their interactions with galectin‐3 as a model lectin is conducted.
Hydrating the Bispropionate Notch in Malaria Pigment: A New Structural Motif in the Iron(III)(deuteroporphyrin) Dimer
Authors: Kuter David , Suárez Liliana , Dodd Erin L. , Noll Bruce C. , Stephens Peter W. , Bohle D. Scott 
Published: November 2018

AbstractTreating deuterohemin, chloro(deuteroporphyrinato)iron(III), with a non‐coordinating base in DMSO/methanol allows for the isolation of [(deuteroporphyrinato)iron(III)]2, deuterohematin anhydride (DHA), an analogue of malaria pigment, the natural product of heme detoxification by malaria. The structure of DHA obtained from this solvent system has been solved by X‐ray powder diffraction analysis and displays many similarities, yet important structural differences, to malaria pigment. Most notably, a water molecule of solvation occupies a notch created by the propionate side chains and stabilizes a markedly bent propionate ligand coordinated with a long Fe−O bond, and a carboxylate cluster associated with water molecules is generated. Together, these features account for its increased solubility and more open structure, with an increased porphyrin–porphyrin separation. The IR spectroscopic signature associated with this structure also accounts for the strong IR band at 1587 cm−1 seen for many amorphous preparations of synthetic malaria pigment, and it is proposed that stabilizing these structures may be a new objective for antimalarial drugs. The important role of the vinyl substituents in this biochemistry is further demonstrated by the structure of deuterohemin obtained by single‐crystal X‐ray diffraction analysis.
Expression of programmed cell death ligand 1 in non–small cell lung cancer: Comparison between cytologic smears, core biopsies, and whole sections using the SP263 assay
Authors: Munari Enrico , Zamboni Giuseppe , Sighele Giorgia , Marconi Marcella , Sommaggio Marco , Lunardi Gianluigi , Rossi Giulio , Cavazza Alberto , Moretta Francesca , Gilioli Eliana , Caliò Anna , Netto George J. , Hoque Mohammad O. , Martignoni Guido , Brunelli Matteo , Vacca Paola , Moretta Lorenzo , Bogina Giuseppe 
Published: November 2018

BackgroundEvaluation of programmed cell death ligand 1 (PD‐L1) expression can be made on both resection specimens and diagnostic biopsies; however, more than 30% of patients with advanced non–small cell lung cancer (NSCLC) do not have adequate histologic material to perform PD‐L1 assays and require additional biopsies. In addition, in our practice, more than 16% of cases have cytological smears as the only available material. Our aim was to validate the PD‐L1 immunocytochemistry assay on cytological smears and compare its accuracy with the results obtained from tissue cores and whole tumor sections using the clinically relevant cutoff of 50%.MethodWe compared the PD‐L1 staining results of cytological smears to those from tissue cores or whole sections in 50 and 53 NSCLC cases, respectively, using the SP263 assay after scanning hematoxylin and eosin slides.ResultsWe found an overall agreement of 90.6% between cytological smears and whole sections; specifically, we found absolute concordance between smears with PD‐L1 expressed in <10% and ≥50% of cells and whole sections with PD‐L1 expressed in <50% and ≥50% of cells, respectively. In addition, slightly lower diagnostic accuracy was found for the cytological smears in comparison with the tissue cores, but the difference was not statistically significant. We found excellent intraobserver and good interobserver agreement in the evaluation of PD‐L1 on smears.ConclusionImmunocytochemistry on cytological smears is a reliable method for determination of PD‐L1 at the 50% cutoff when positive cells are <10% or ≥50%; for cases showing PD‐L1 expression in 10% to 49% of cells, additional tissue sampling may be necessary.
The three‐axial gyroscope sensor detects the turning point between opening and closing phases of chewing
Authors: Minami Ichiro , Wirianski Alex , Harakawa Ryosuke , Wakabayashi Noriyuki , Murray Greg M. 
Published: November 2018

AbstractMost devices measuring the kinematics of masticatory function are cumbersome to setup and not portable. Data collection would be facilitated, particularly in the elderly, if the device used for the objective measurement of mastication was easily transportable and simple to setup. Accelerometers and gyroscope sensors are lightweight and portable and may be useful alternatives. The definition of the turning point between the opening and closing phases of chewing is important for studies of associations between muscle activity and effects of perturbations. Measures of the mediolateral angle (specifically, the mandibular tilt from the lateral view) allow the detection of the turning point between the opening and closing phases. The aim was to determine whether a three‐axial gyroscope sensor can detect the turning point between opening and closing phases of chewing. Fourteen asymptomatic participants chewed gum while the output was recorded from a three‐axial gyroscope sensor (Seiko Epson, Japan) attached to the chin and a 6 degree‐of‐freedom electromagnetic jaw‐tracking device (Pollhemus, USA). Bland–Altman plots were used to assess the matching of the recordings made by the three‐axial gyroscope sensor and the jaw‐tracking device. The turning points between the opening and closing phases of chewing matched closely when recorded by a jaw‐tracking device and when using a three‐axial gyroscope sensor. A three‐axial gyroscope sensor can validly detect the turning point between the opening and closing phases during chewing of gum.
Investigation of the Reaction Pathways of Biomass‐Derived Oxygenate Conversion into Monoalcohols in Supercritical Methanol with CuMgAl‐Mixed‐Metal Oxide
Authors: Galebach Peter H. , Thompson Sean , Wittrig Ashley M. , Buchanan J. Scott , Huber George W. 
Published: November 2018

AbstractInvited for this month's cover is the group of George Huber at the University of Wisconsin‐Madison. The cover picture shows the chemical intermediates and final products for the catalytic conversion of wood into C2–C6 alcohols over a CuMgAl oxide catalyst in methanol. The Full Paper itself is available at 10.1002/cssc.201801816.
Design and synthesis of simplified speciophylline analogues and β‐carbolines as active molecules against Plasmodium falciparum
Authors: Pierrot David , Sinou Véronique , Bun Sok‐Siya , Parzy Daniel , Taudon Nicolas , Rodriguez Jean , Ollivier Evelyne , Bonne Damien 
Published: November 2018

AbstractA structure–activity relationship study of active molecules against chloroquine‐resistant Plasmodium falciparum K1 strain is reported. Structurally simplified analogues of antiplasmodial active alkaloids presented similar levels of activity as their corresponding natural products extracted from Guiera senegalensis and Mitragyna inermis with IC50 values on chloroquine‐resistant P. falciparum K1 strain of up to 10.6 μM for spirooxindoles and 13.8 μM for β‐carbolines. The identification of such simpler and cheaper structural analogues is crucial to efficiently study these natural products' action mode as well as developing new cures against malaria.
Photochemical Synthesis of cis,trans,cis‐1,2,3,4‐Tetrakis(diphenylphosphanyl)buta‐1,3‐diene and Its Metal Coordination
Authors: Prock Johannes , Ehrmann Katharina , Viertl Wolfgang , Pehn Richard , Pann Johann , Roithmeyer Helena , Bendig Marvin , Rodríguez Villalón Alba , Kopacka Holger , Dumfort Alexander , Oberhauser Werner , Clausing Simon T. , Knör Günther , Brüggeller Peter 
Published: November 2018

AbstractInvited for the cover of this issue is the group of Peter Brüggeller from the University of Innsbruck, Austria. The cover image shows the multi‐step synthesis of dppbd.
Peer Review of Mendeleev’s 1869 Breakthrough Paper: ‘I Suggest Eliminating the Table…’
Authors: Seeman Jeffrey I. 
Published: November 2018

AbstractThis article describes a document sent in 1982 by Jack Dunitz at the Eidgenössische Technische Hochschule Zürich to Roald Hoffmann at Cornell University, reportedly a Journal of the American Chemical Society peer review of Dmitri Mendeleev’s 1869 submission on the periodic table. Several aspects of the resultant mystery are examined.
Use of a criteria‐based audit to optimize uptake of cesarean delivery in a low‐resource setting
Authors: Mgaya Andrew H. , Kidanto Hussein L. , Nyström Lennarth , Essén Birgitta 
Published: November 2018

AbstractObjectiveTo evaluate the impact of a criteria‐based audit (CBA) of obstructed labor and fetal distress on cesarean delivery and perinatal outcomes.MethodsA cross‐sectional study was performed at a tertiary referral hospital in Tanzania. Data were collected before and after CBA (January 2013–November 2013 and July 2015–June 2016). Outcomes of fetal distress (baseline CBA, n=248; re‐audit, n=251) and obstructed labor (baseline CBA, n=260; re‐audit n=250) were assessed using a checklist. Additionally, 27 960 parturients were assessed using the Robson classification.ResultsPerinatal morbidity and mortality decreased from 42 of 260 (16.2%) to 22 of 250 (8.8%) among patients with obstructed labor after CBA (P=0.012). Cesarean delivery rate decreased for referred term multiparas with induced labor or prelabor cesarean delivery (odds ratio [OR] 0.28, 95% confidence interval [CI] 0.09–0.82). Cesarean delivery rate for preterm pregnancies increased among both referred (OR 1.28, 95% CI 1.02–1.63) and non‐referred (OR 2.78, 95% CI 1.98–3.90) groups. Neonatal distress rate decreased for referred term multiparas (OR 0.72, 95% CI 0.56–0.92), referred preterm pregnancies (OR 0.32, 95% CI 0.25–0.39), and non‐referred preterm pregnancies (OR 0.26, 95% CI 0.18–0.36).ConclusionUse of CBA reduced poor perinatal outcomes of obstructed labor and increased uptake of cesarean delivery.
Interaction of lncRNA‐MALAT1 and miR‐124 regulates HBx‐induced cancer stem cell properties in HepG2 through PI3K/Akt signaling
Authors: He Bo , Peng Feng , Li Wei , Jiang Yongfang 
Published: November 2018

AbstractBackgroundHepatitis B virus X protein (HBx) plays a crucial role in initiating and promoting HBV‐induced hepatocellular carcinoma (HCC) development. Reports indicated that HBx promotes cancer stem cell (CSC) generation, which may be associated with HBV‐related HCC. Noncoding RNA miR‐124 and long noncoding RNA (lncRNA)‐metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) were considered to be involved deeply in the progress of HBx‐related HCC. Hence, the underlying mechanism of miR‐124 and lncRNA‐MALAT1 in regulating HBx‐promoted CSC needs to be studied.Materials and MethodsIn present study, HepG2‐X cell line was induced by transfect HBx into HepG2 cells. Overexpressing of miR‐124 or silencing of lncRNA‐MALAT1 was completed by transfecting miR‐124 mimic or shMALAT1 into HepG2‐X cells. HBx‐induced CSC properties and tumorigenic potential of HepG2 cells were determined by detecting CSC marker expression, colony formation assay, and xenograft tumorigenesis. The mechanism of HBx‐induced CSC properties was explored by PI3K/Akt inhibitor. Interaction of miR‐124 and lncRNA‐MALAT1 was detected by luciferase reporter assay.ResultsHBx promoted CSC properties through upregulating stemness markers and reprogramming proteins, and contributed to tumorigenicity of HepG2‐X cells both in vivo and in vitro. Inhibition of Akt activation blocked the HBx‐stimulated reprogramming proteins and stemness markers. HBx upregulated lncRNA‐MALAT1 expression while downregulating miR‐124 expression in HepG2‐X cells. miR‐124 interacts with lncRNA‐MALAT1 by direct targeting. Overexpression of miR‐124 or silencing of lncRNA‐MALAT1 both blocked HBx‐induced CSC generation, stemness‐related factor activation and tumorigenicity via PI3K/Akt signaling.ConclusionOur results demonstrated that miR‐124 interact with lncRNA‐MALAT1 and involve in regulating HBx‐induced CSC properties via PI3K/Akt signaling.
RETRACTED: miR‐625‐5p/PKM2 negatively regulates melanoma glycolysis state
Authors: Zhang Hailin , Feng Cheng , Zhang Mingzi , Zeng Ang , Si Loubin , Yu Nanze , Bai Ming 
Published: November 2018

AbstractPKM2 plays an important role in cancer glycolysis, however, the link of PKM2 and microRNAs (miRNAs) in melanoma is still unclear. The study will investigate the role of miRNAs in regulating PKM2 mediated melanoma cell glycolysis. We found that high PKM2 expression in melanoma tissues and cell lines was positively associated with glycolysis. Further study indicated that miR‐625‐5p regulated PKM2 expression on mRNA and protein levels in melanoma cells. There was a negative relationship between miR‐625‐5p and PKM2 expression in the clinical melanoma samples. These findings provide an evidence that miR‐625‐5p/PKM2 plays a role in melanoma cell glucose metabolism.
Cellular and molecular mechanisms of sarcopenia: the S100B perspective
Authors: Riuzzi Francesca , Sorci Guglielmo , Arcuri Cataldo , Giambanco Ileana , Bellezza Ilaria , Minelli Alba , Donato Rosario 
Published: November 2018

AbstractPrimary sarcopenia is a condition of reduced skeletal muscle mass and strength, reduced agility, and increased fatigability and risk of bone fractures characteristic of aged, otherwise healthy people. The pathogenesis of primary sarcopenia is not completely understood. Herein, we review the essentials of the cellular and molecular mechanisms of skeletal mass maintenance; the alterations of myofiber metabolism and deranged properties of muscle satellite cells (the adult stem cells of skeletal muscles) that underpin the pathophysiology of primary sarcopenia; the role of the Ca2+‐sensor protein, S100B, as an intracellular factor and an extracellular signal regulating cell functions; and the functional role of S100B in muscle tissue. Lastly, building on recent results pointing to S100B as to a molecular determinant of myoblast–brown adipocyte transition, we propose S100B as a transducer of the deleterious effects of accumulation of reactive oxygen species in myoblasts and, potentially, myofibers concurring to the pathophysiology of sarcopenia.
Analysis of differences in laxities and neutral positions from native after kinematically aligned TKA using cruciate retaining implants
Authors: Roth Joshua D. , Howell Stephen M. , Hull Maury L. 
Published: November 2018

ABSTRACTOne biomechanical goal of kinematically aligned total knee arthroplasty (KA TKA) is to achieve knee laxities and neutral positions that are not different from those of the native knee without soft tissue release. However, replacing the articular surfaces and menisci with implants of discrete sizes and average shapes and resecting the anterior cruciate ligament (ACL) might prevent KA TKA from achieving this goal in the tibiofemoral joint. Accordingly, the objective was to determine whether either or both surgically induced changes cause differences in laxities and/or neutral positions from native using a cruciate retaining implant. Eight laxities and four neutral positions were measured from 0° to 120° flexion in 30° increments in 13 human cadaveric knees in three knee conditions: native, ACL‐deficient, and KA TKA. After KA TKA, 5 of the 40 laxity measures (8 laxities × 5 flexion angles) and 6 of the 20 neutral position measures (4 neutral positions × 5 flexion angles) were statistically different from those of the native knee. The greatest differences in laxities from native after KA TKA occurred at 30° flexion in anterior translation (1.6 ± 2.1 mm increase, p < 0.0001); this difference was 1.7 ± 2.1 mm less than that in the ACL‐d knee (p < 0.0001). The greatest difference in neutral positions from native after KA TKA occurred in anterior–posterior translation at 0° flexion (3.8 ± 1.9 mm anterior, p < 0.0001); this difference was 2.6 ± 1.9 mm greater than that in the ACL‐d knee (p = 0.0002). Clinical Significance: These results indicate that the biomechanical goal of KA TKA is largely realized despite the two surgically induced changes. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:358–369, 2019.
Membrane proteome characterization of periodontal ligament cell sets from deciduous and permanent teeth
Authors: Giovani Priscila A. , Salmon Cristiane R. , Martins Luciane , Leme Adriana F. P. , Puppin‐Rontani Regina M. , Mofatto Luciana S. , Nociti Francisco H. , Kantovitz Kamila R. 
Published: November 2018

AbstractBackgroundPhysiological roles for the periodontal ligament (PDL) include tooth eruption and anchorage, force absorption, and provision of proprioceptive information. Despite the advances in understanding the biology of PDL cells, there is a lack of information regarding the molecular signature of deciduous (DecPDL) and permanent (PermPDL) PDL tissues. Thus, the present study was designed to characterize the membrane proteome of DecPDL and PermPDL cells.MethodsPrimary PDL cells were obtained (n = 6) and a label‐free quantitative proteome of cell membrane‐enriched components was performed. Proteome findings were validated by quantitative polymerase chain reaction and Western blot assays in fresh human tissues (n = 8) and primary cell cultures (n = 6). In addition, confocal microscopy was used to verify the expression of target factors in the PDL cell cultures.ResultsComparative gene ontology enrichment analysis evidenced that most stickling differences involved “endomembrane system” (PICALM, STX4, and LRP10), “hydrolase activity” (NCSTN and XRCC6), “protein binding” (PICALM, STX4, GPNMB, VASP, extended‐synaptotagmin 2 [ESYT2], and leucine‐rich repeat containing 15 [LRRC15]), and “isomerase activity” (FKBP8). Data are available via ProteomeXchange with identifier PXD010226. At the transcript level, high PICALM in DecPDL and ESYT2 and LRRC15 in PermPDL were confirmed in fresh PDL tissues. Furthermore, Western blot analysis confirmed increased levels of PICALM, LRRC15, and ESYT2 in cells and/or fresh tissues, and confocal microscopy confirmed the trends for PICALM and LRRC15 expression in PDL cells.ConclusionWe report the first comprehensive characterization of the membrane protein machinery of DecPDL and PermPDL cells, and together, we identified a distinct molecular signature for these cell populations, including unique proteins for DecPDL and PermPDL.