bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of UpholdTM Vaginal Mesh Procedure with Hysterectomy or Uterine Preservation for the Treatment of Pelvic Organ Prolapse
Authors: Ker Chin-Ru , Lin Kun-Ling , Loo Zi-Xi , Juan Yung-Shun , Long Cheng-Yu 
Published: June 2018

AbstractHysterectomy is the empirical treatment for female pelvic organ prolapse (POP) without robust evidence to prove its efficacy. Uphold transvaginal mesh (TVM) system is an innovated device with smaller area, superior anterior/apical support with single incision to treat POP. The prospective cohort study aims to evaluate Uphold TVM’s performance with or without concomitant hysterectomy. Inclusion criteria specify patients suffering from stage 2 or more anterior/apical prolapse without previous hysterectomy. Exclusion criteria specify those with contraindications to uterine preservation, such as leiomyomas, adenomyosis, endometrial hyperplasia, abnormal uterine bleeding, postmenopausal bleeding, cervical dysplasia, receiving tamoxifen treatment, family history of gynecology cancer, or colon cancer and incapability to be routinely followed. Thirty patients are recruited in the hysterectomy group and 66 patients in the hysteropexy group. The results demonstrate that patients with Uphold mesh only experience longer vaginal length, shorter operation duration, less blood loss and less post-operation pain. The performance in anatomical correction, lower urinary tract symptoms improvement, de novo dyspareunia, urodynamic study parameters and mesh extrusion rates are comparable with or without uterus preservation. The information is useful in pre-operation counseling, when the patient can make an educated choice whether or not to receive concomitant hysterectomy.
Characterization of a community-acquired-MRSA USA300 isolate from a river sample in Austria and whole genome sequence based comparison to a diverse collection of USA300 isolates
Authors: Lepuschitz Sarah , Huhulescu Steliana , Hyden Patrick , Springer Burkhard , Rattei Thomas , Allerberger Franz , Mach Robert L. , Ruppitsch Werner 
Published: June 2018

AbstractThe increasing emergence of multi-resistant bacteria in healthcare settings, in the community and in the environment represents a major health threat worldwide. In 2016, we started a pilot project to investigate antimicrobial resistance in surface water. Bacteria were enriched, cultivated on selective chromogenic media and species identification was carried out by MALDI-TOF analysis. From a river in southern Austria a methicillin resistant Staphylococcus aureus (MRSA) was isolated. Whole genome sequence analysis identified the isolate as ST8, spa type t008, SCCmecIV, PVL and ACME positive, which are main features of CA-MRSA USA300. Whole genome based cgMLST of the water isolate and comparison to 18 clinical MRSA USA300 isolates from the Austrian national reference laboratory for coagulase positive staphylococci originating from 2004, 2005 and 2016 and sequences of 146 USA300 isolates arbitrarily retrieved from the Sequence Read Archive revealed a close relatedness to a clinical isolate from Austria. The presence of a CA-MRSA USA300 isolate in an aquatic environment might pose a public health risk by serving as a potential source of infection or a source for emergence of new pathogenic MRSA clones.
Dispersal-competition tradeoff in microbiomes in the quest for land colonization
Authors: Dini-Andreote Francisco , van Elsas Jan Dirk , Olff Han , Salles Joana Falcão 
Published: June 2018

AbstractAncestor microbes started colonizing inland habitats approximately 2.7 to 3.5 billion years ago. With some exceptions, the key physiological adaptations of microbiomes associated with marine-to-land transitions have remained elusive. This is essentially caused by the lack of suitable systems that depict changes in microbiomes across sufficiently large time scales. Here, we investigate the adaptive routes taken by microbiomes along a contemporary gradient of land formation. Using functional trait-based metagenomics, we show that a switch from a microbial ‘dispersal’ to a ‘competition’ responsemodusbest characterizes the microbial trait changes during this eco-evolutionary trajectory. The ‘dispersal’modusprevails in microbiomes at the boundary sites between land and sea. It encompasses traits conferring cell chemosensory and motile behaviors, thus allowing the local microbes to exploit short-lived nutritional patches in high-diffusion microhabitats. A systematic transition towards the ‘competition’modusoccurs progressively as the soil matures, which is likely due to forces of viscosity or strain that favor traits for competition and chemical defense. Concomitantly, progressive increases in the abundances of genes encoding antibiotic resistance and complex organic substrate degradation were found. Our findings constitute a novel perspective on the ecology and evolution of microbiome traits, tracking back one of the most seminal transitions in the evolutionary history of life.
Rare structural variants in the DOCK8 gene identified in a cohort of 439 patients with neurodevelopmental disorders
Authors: Krgovic Danijela , Kokalj Vokac Nadja , Zagorac Andreja , Gregoric Kumperscak Hojka 
Published: June 2018

AbstractDetection of copy number variations (CNVs) is a first-tier clinical diagnostic test for children with neurodevelopmental disorders (NDD), which reveals the genetic cause of the disorder in more than 20%. These are mostly known microdeletion/microduplication syndromes, but variants of unknown clinical significance (VOUS) and ambiguous CNVs can also be detected. An example of the last two are abnormalities in the DOCK8 gene. Conflicting interpretations of CNVs affecting DOCK8 can be found in the literature. Deletions were predicted to have a impact in carriers with variable clinical manifestations, where duplications have been proposed as benign variants. In our study, CNV screening was performed in a cohort involving 439 probands with suspected NDD. We identified known microdeletion/microduplication syndromes in 19% and VOUS CNVs in 8% of patients. Among these, three patients had a CNV encompassing the DOCK8 gene. Although diverse clinical presentations are noted in our three patients, comparison of their phenotypes revealed that abnormalities in cognition and communication, aggressive behaviour and mood swings are common to all of them. Therefore, a clinical relevance, in terms of influencing the psychiatric clinical picture of patients, is proposed for the CNVs disrupting the DOCK8 gene, regardless of whether it is a deletion or duplication.
Suppressed topological phase transitions due to nonsymmorphism in SnTe stacking
Authors: Araújo Augusto L. , Ferreira Gerson J. , Schmidt Tome M. 
Published: June 2018

AbstractWe combine first principles calculations with a group theory analysis to investigate topological phase transitions in the stacking of SnTe monolayers. We show that distinct finite stacking yields different symmetry-imposed degeneracy, which dictates the hybridization properties of opposite surface states. For SnTe aligned along the [001] direction, an (even) odd number of monolayers yields a (non)symmorphic space group. For the symmorphic case, the hybridization of surface states lead to band inversions and topological phase transitions as the sample height is reduced. In contrast, for a nonsymmorphic stacking, an extra degeneracy is guaranteed by symmetry, thus avoiding the hybridization and topological phase transitions, even in the limit of a few monolayers. Our group theory analysis provide a clear picture for this phenomenology and matches well the first principles calculations.
Genomic analysis of a Raoultella ornithinolytica strain causing prosthetic joint infection in an immunocompetent patient
Authors: Beye Mamadou , Hasni Issam , Seng Piseth , Michelle Caroline , La Scola Bernard , Raoult Didier , Fournier Pierre-Edouard 
Published: June 2018

AbstractWe sequenced the genome of Raoultella ornithinolytica strain Marseille-P1025 that caused a rare case of prosthetic joint infection in a 67-year-old immunocompetent male. The 6.7-Mb genome exhibited a genomic island (RoGI) that was unique among R. ornithinolytica strains. RoGI was likely acquired by lateral gene transfer from a member of the Pectobacterium genus and coded for a type IVa secretion system found in other pathogenic bacteria and that may have conferred strain Marseille-P1025 an increased virulence. Strain Marseille-P1025 was also able to infect, multiply within, and kill Acanthamoaeba castellanii amoebae.
A widely-applicable high-throughput cellular thermal shift assay (CETSA) using split Nano Luciferase
Authors: Martinez Natalia J. , Asawa Rosita R. , Cyr Matthew G. , Zakharov Alexey , Urban Daniel J. , Roth Jacob S. , Wallgren Eric , Klumpp-Thomas Carleen , Coussens Nathan P. , Rai Ganesha , Yang Shyh-Ming , Hall Matthew D. , Marugan Juan J. , Simeonov Anton , Henderson Mark J. 
Published: June 2018

AbstractAssessment of the interactions between a drug and its protein target in a physiologically relevant cellular environment constitutes a major challenge in the pre-clinical drug discovery space. The Cellular Thermal Shift Assay (CETSA) enables such an assessment by quantifying the changes in the thermal stability of proteins upon ligand binding in intact cells. Here, we present the development and validation of a homogeneous, standardized, target-independent, and high-throughput (384- and 1536-well formats) CETSA platform that uses a split Nano Luciferase approach (SplitLuc CETSA). The broad applicability of the assay was demonstrated for diverse targets, and its performance was compared with independent biochemical and cell-based readouts using a set of well-characterized inhibitors. Moreover, we investigated the utility of the platform as a primary assay for high-throughput screening. The SplitLuc CETSA presented here enables target engagement studies for medium and high-throughput applications. Additionally, it provides a rapid assay development and screening platform for targets where phenotypic or other cell-based assays are not readily available.
Aqueous supercapacitors based on carbonized silk electrodes
Authors: Zhang Limei , Meng Zhaohui , Qi Qiaoyun , Yan Wen , Lin Naibo , Liu Xiang Yang 
Published: June 2018

An aqueous symmetrical supercapacitor was achieved by assembling SC-850 electrodes, which possess a specific energy of 14.33 W h kg−1 at a power density of 251 W kg−1 operated over the wide voltage range of 2.0 V in aqueous neutral Na2SO4 electrolyte.
Timing offset calibration of CZTI instrument aboard ASTROSAT
Authors: Basu A. , Joshi B. C. , Bhattacharya D. , Rao A. R. , Naidu A. , Krishnakumar M. A. , Arumugsamy P. , Vadawale S. , Manoharan P. K. , Dewangan G. C. , Vibhute A. , Mithun N. P. S. , Sharma V. 
Published: June 2018

Aim: Both the radio and the high-energy emission mechanism in pulsars is not yet properly understood. A multiwavelength study is likely to help better understand of such processes. ASTROSAT, the first Indian space-based observatory, has five instruments aboard that cover the electromagnetic spectrum from infra-red (1300 Å) to hard X-ray (380 keV). The instrument relevant to our study is the Cadmium Zinc Telluride Imager (CZTI). CZTI is a hard X-ray telescope functional over an energy range of 20–380 keV. We aim to estimate the timing offset introduced in the data acquisition pipeline of the instrument, which will help in time alignment of high energy time-series with those from two other ground based observatories, viz. the Giant Meterwave Radio Telescope (GMRT) and the Ooty Radio Telescope (ORT).
Method: PSR B0531+21 is a well studied bright pulsar with closely aligned radio and hard X-ray pulse profiles. We used simultaneous observations of this pulsar with the ASTROSAT, the ORT, and the GMRT. As the pulsar resides in a very turbulent environment and shows significant timing noise, it was specially observed using the ORT with almost daily cadence to obtain good timing solutions. We also supplemented the ORT data with archival Fermi data for estimation of timing noise. We obtained the phase connected timing solution of the pulsar by estimating its dispersion measure variations and the timing noise. The timing offset of ASTROSAT instruments was estimated from fits to pulse arrival time data at the ASTROSAT and the radio observatories.
Results: We estimate the relative offset of ASTROSAT-CZTI with respect to GMRT to be −4716 ± 50 μs. The corresponding offset with the ORT was −29639 ± 50 μs and Fermi-LAT was −5368 ± 56 μs respectively.
Review: Endoplasmic Reticulum-Associated Degradation (ERAD)-Dependent Control of (Tri)terpenoid Metabolism in Plants
Authors: Erffelinck Marie-Laure , Goossens Alain 
Published: June 2018

AbstractPlants are sessile organisms. Therefore, they developed the capacity to quickly respond to biotic and abiotic environmental stresses, for instance by producing a broad spectrum of bioactive specialized metabolites. In this defense response, the jasmonate phytohormones can instigate a signaling cascade that leads to the specific elicitation and reprograming of numerous metabolic pathways. Recent research progress has provided several insights into the regulatory networks of many specialized metabolic pathways, mainly at the transcriptional level. Nonetheless, our view on the regulation of defense metabolism remains far from comprehensive. Here, we describe the recent advances obtained with regard to one aspect of the regulation of plant specialized metabolism, namely the posttranslational regulation of enzyme stability. We focus on terpenoid biosynthesis and in particular on the rate-limiting and well-investigated enzyme of the terpenoid precursor pathway, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR). There are clear similarities, as well as important mechanistic differences, among the components involved in the posttranslational regulation of terpenoid biosynthesis via HMGR in plants, yeasts, and mammals. Furthermore, in plants, several of these components evolved to respond to specific signaling cues. Indeed, the elements of the plant endoplasmic reticulum-associated degradation (ERAD) and ER stress-associated processes can be induced upon environmental stresses and during specific developmental processes, thereby allowing a unique posttranslational regulation of terpenoid biosynthesis pathways.
Benefits from, Satisfaction with, and Self-Efficacy for Advanced Digital Hearing Aids in Users with Mild Sensorineural Hearing Loss
Authors: Jilla Anna , Danhauer Jeffrey , Sullivan J. , Sanchez Kristin , Johnson Carole 
Published: June 2018

AbstractLittle evidence is available regarding outcomes of advanced digital technology (ADT) hearing aid wearers with mild sensorineural hearing loss (MSNHL). The purpose of this article is to report the characteristics of and outcomes for this population. A cross-sectional research design was employed with 56 participants from a private practice setting. The International Outcomes Inventory for Hearing Aids (IOI-HA), Satisfaction with Amplification in Daily Life (SADL), and the Measure of Audiologic Rehabilitation Self-Efficacy for Hearing Aids (MARS-HA) were completed, scored, and compared with normative data. Results revealed that ADT aids were worn 10.5 hours/day, were mostly advanced to premium (55%), had an average cost per aid of $2,138 (SD = $840), and provided significant benefit (IOI-HA overall score: mean = 4.1; SD = 0.6) and satisfaction (SADL global score: mean = 5.4; SD = 0.8) to users who had good overall self-efficacy (MARS-HA composite score: mean = 81.7; SD = 12.8). Patients were most dissatisfied with and had the least self-efficacy for managing background noise and advanced handling of their devices. ADT hearing aid users with MSNHL achieved excellent outcomes, but ongoing follow-up and counseling from hearing health care providers may be important for successful management of background noise and mastery of advanced handling skills.
Effects of Including Information about Hidden Hearing Loss in an Adopt-A-Band Program on College Band Members' Attitudes toward Healthy Hearing Behaviors
Authors: Johnson Carole , Baldwin Jonathan , Danhauer Jeffrey , Wolfe Brian , Jeannont Stevana , Seever Katie 
Published: June 2018

AbstractYoung musicians may be at risk for developing cochlear synaptopathy (CS), or hidden hearing loss (HHL), that could lead to permanent music-induced hearing loss (MIHL). Patients with CS often complain of tinnitus and/or difficulty understanding speech in noisy situations, even though traditional audiometric testing indicates normal hearing. The aim of this article was to determine the effects of including information about HHL on an Adopt-A-Band program involving college band members' concern about and self-efficacy toward the prevention of MIHL. We conducted a single-blinded, randomized clinical trial. Forty-eight band members participated in this study. Band members were randomly assigned to two Adopt-A-Band presentations, one with and one without information on HHL. Including information about HHL had no effect on these band members' concerns about and self-efficacy toward the prevention of MIHL. However, the Adopt-A-Band program resulted in significantly increased concern for MIHL by 39.5% (p < 0.0001, 95% confidence interval [CI]: 25–54.2), self-efficacy in its prevention by 79.1% (p < 0.0001, 95% CI: 66.9–91.2), and plans to use musicians' earplugs while playing by 67.4% (p < 0.0001, 95% CI: 53.4–81.45). Although inclusion of information about HHL did not have a significant effect, the Adopt-A-Band program, in general, significantly increased the immediate intent of these students to practice healthy hearing behaviors. Future research is needed to determine the long-term effects of using the Adopt-A-Band program with university marching bands' use of healthy hearing behaviors.
Gaps in health information exchange between hospitals that treat many shared patients
Authors: Everson Jordan , Adler-Milstein Julia 
Published: June 2018

Abstract

Objective
Hospitals that routinely share patients are those that most critically need to engage in electronic health information exchange (HIE) with each other to ensure clinical information is available to inform treatment decisions. We surveyed pairs of hospitals in a nationwide sample to describe whether and how hospitals within each hospital referral region (HRR) that have the highest shared patient (HSP) volume engaged in HIE with each other.

Methods
We used Medicare’s Physician Shared Patient Patterns data to identify hospital pairs with the highest shared patient volume in each hospital referral region. We surveyed a purposeful sample of pairs and then calculated descriptive statistics to compare: (1) HIE with the HSP hospital vs HIE with other hospitals, and (2) HIE with the HSP hospital versus federal measures of HIE engagement that are not partner-specific.

Results
We received responses from 25.5% of contacted hospitals and 33.5% of contacted pairs, allowing us to examine information sharing among 68 hospitals in 63 pairs. 23% of respondents reported worse information sharing with their HSP hospital than with other hospitals while 17% indicated better sharing with their HSP hospital and 48% indicated no difference. Our HSP-specific measures of HIE differed from federal measures of HIE engagement: while 97% of respondents are classified as routinely sending information electronically in federal measures, in our data only 63% did so with their HSP hospital.

Conclusions
Despite increased HIE engagement, our descriptive results indicate that HIE is not developing in a way that facilitates information exchange where it might benefit the most patients. New policy efforts, particularly those emerging from the 21st Century Cures Act, need to explicitly pursue strategies that ensure that HSP providers engage in exchange with each other.
Oxford Handbook of Oral and Maxillofacial Surgery
Authors: Cascarini Luke , Schilling Clare , Gurney Ben , Brennan Peter 
Published: June 2018

Abstract
This new edition of Oxford Handbook of Oral and Maxillofacial Surgery has been fully updated to cover the current guidelines and research in the field of OMFS. Splitting vital knowledge into sections based on clinical areas, this handbook uses bulleted lists and summary boxes to make the information easily searchable. Chapters on ‘in the clinic’, ‘in the theatre’, and ‘on the ward’ cover all common complaints and presentations that the reader can expect to encounter in their daily activities, and a dedicated section to emergencies provides clear advice. Common drugs and dental materials are covered as a quick reference guide. With OMFS now part of the Core Training programme for surgical trainees, the handbook ensures a solid grasp of the basics and fundamentals to help support decision making for junior doctors, dental foundation trainees, specialist nurses, and medical and dental students.
Incidence and Outcomes of Positive Donor Rim Cultures and Infections in the Cornea Preservation Time Study
Authors: Mian Shahzad I. , Aldave Anthony J. , Tu Elmer Y. , Ayres Brandon D. , Jeng Bennie H. , Macsai Marian S. , Nordlund Michael L. , Penta Jeffrey G. , Pramanik Sudeep , Szczotka-Flynn Loretta B. , Ayala Allison R. , Liang Wendi , Maguire Maureen G. , Lass Jonathan H. ,   on behalf of the Cornea Preservation Time Study Group
Published: June 2018

Purpose:
To assess donor rim culture results and outcomes of ocular infections in the Cornea Preservation Time Study (CPTS).

Methods:
Donor corneal rim cultures were optional. Donor characteristics were assessed for association with positive cultures using the Fisher exact test and Poisson regression analyses. Incidence rates of ocular infections were estimated, and 95% confidence intervals were calculated.

Results:
Cultures were performed in 784 (58.9%) of the 1330 cases. For the 0 to 7-day versus 8 to 14-day preservation time groups, respectively, positive fungal growth occurred in 10 of 397 (2.5%) versus 5 of 387 (1.3%) corneas (P = 0.30), whereas positive bacterial cultures occurred in 6 of 397 (1.5%) versus 4 of 387 (1.0%) corneas (P = 0.75). Surgeon-prepared tissue remained a significant risk for positive fungal cultures [relative risk (RR) of surgeon- versus eye-bank-prepared, 2.85; 95% CI (1.02–7.98)], whereas younger donors [RR per year of age, 0.96; 95% CI (0.93–1.00)] and accidental death donors [RR of accident versus disease, 3.71; 95% CI (1.36–10.13)] were at a greater risk for positive bacterial cultures. Fungal infection (Candida glabrata) developed in 1 (6.7%) of 15 recipients with a positive fungal culture, and no recipient infections occurred with positive bacterial culture. With one additional fungal keratitis (Candida albicans) and one bacterial endophthalmitis (E. coli) with no rim culture performed, a total of 2 of 1330 eyes (0.15%) developed fungal and 1/1330 eyes (0.08%) developed bacterial postkeratoplasty infections.

Conclusions:
A longer preservation time was not associated with a higher rate of positive donor rim cultures. The overall rate of infection across the entire cohort was low.