bluuebunny/crossref_metadata_2025_split huggingface dataset:
Graft failure of IgA nephropathy in renal allografts following living transplantation: predictive factor analysis of 102 biopsies
Authors: Zhang Jin , Chen Guo-dong , Qiu Jiang , Liu Guo-chang , Chen Li-zhong , Fu Kai , Wu Zi-xuan 
Published: August 2019

Abstract
Background

To investigate predictive factors related to graft failure of IgA nephropathy(IgAN) in renal allografts following living transplantation.

Methods

We identified a series of 102 biopsies diagnosed as IgAN in renal allografts following living transplantation from July 2004 to January 2017 at our center, and assess the predict value of the Lee's classification and the 2009 Oxford classification in IgAN in renal allografts, clinical, ultrasonic and pathological characteristics at biopsy and the outcomes were retrospectively analyzed.

Results

The 5-year graft cumulative survival rate after transplantation was 91.4%. The 4-year graft cumulative survival rate after biopsy diagnosis of IgAN in renal allografts was 59.6%. The mean time ± SD to disease was 4.7 ± 3.5 years. The color doppler ultrasound and blood flow imagine showed the echo enhancement, the reduced blood flow distribution, the reduced peak systolic velocity of main renal artery, and the increased resistance index of arcuate renal artery were valuable in evaluating the graft dysfunction. The Cox multivariate analysis revealed that the 24-h urinary protein level (HR 1.6 for 1-g increase, 95%CI 1.2-2.0), estimated glomerular filtration rate (eGFR) (HR 1.0 for 1-mL/min/1.73m^2 decline, 95%CI 1.0-1.1), and mesangial C1q deposition (HR 3.0, 95%CI 1.2-7.4) at biopsy were independent predictive factors of graft failure of IgAN in renal allografts.

Conclusions

IgAN in renal allografts occurred frequently within 5 years after transplantation. The risk of graft failure should be taken seriously in patients who exhibit heavy proteinuria and/or a declined eGFR as the initial symptoms; a high lesion grade (grade IV-V of Lee’s classification) and/or mesangial C1q deposition may also indicated a poor outcome.
Perceived Barriers to Early Diagnosis of Breast Cancer in South and South Western Ethiopia: a qualitative study
Authors: Getachew Sefonias , Tesfaw Aragaw , Kaba Mirgissa , Wienke Andreas , Taylor Lesley , Kantelhardt Eva J. , Addissie Adamu 
Published: August 2019

Abstract
Background Early diagnosis is a key determinant of breast cancer prognosis and survival. More than half of breast cancer cases are diagnosed at an advanced stage in Ethiopia, and the barriers to early diagnosis in this country are not well understood. We aimed to identify the perceived barriers to early diagnosis of breast cancer from the perspective of patients and health care providers in south and southwestern Ethiopia. Methods A qualitative study was conducted from March to April 2018 using in-depth interviews of breast cancer patients and breast cancer health care providers from six public hospitals located in urban and rural areas of south and southwestern Ethiopia. All participants proved verbal consent before participating. A thematic analysis was performed using Open Code 4.02. Results Twelve breast cancer patients and thirteen health care providers were included in the study. Patient and health-system related barriers to early diagnosis of breast cancer were identified. Patient-related barriers were lack of knowledge and awareness of breast cancer, belief in traditional medicine and religious practices for treatment, and lack of social and financial support to seek care at a medical facility. Health-system related barriers were misdiagnosis of breast cancer, long distance to referral facilities, high cost of diagnostic services, long waiting time for diagnostic tests, and lack of screening and diagnostic tests in local facilities. Conclusions Early diagnosis of breast cancer is affected by multiple barriers in south and southwestern Ethiopia. Awareness campaigns and education about the disease, prevention, and early detection are needed to increase early diagnosis of breast cancer. Opportunities exist to improve early diagnosis and timely treatment in rural areas.
Patient-individual cancer cell lines and tissue analysis delivers no evidence for a role of DNA virus infection on colorectal cancer oncogenesis
Authors: Gock Michael , Kordt Marcel , Matschos Stephanie , Mullins Christina S. , Linnebacher Michael 
Published: August 2019

Abstract
Background

Several DNA viruses are highly suspicious to have oncogenic effects in humans. This study investigates the presence of potentially oncogenic viruses such as SV40, JCV, BKV and EBV in patient-derived colorectal carcinoma (CRC) cells typifying all molecular subtypes of CRC.

Methods

Sample material (gDNA and cDNA) of a total of 49 patient-individual CRC cell lines and corresponding primary material from 11 patients, including normal, tumor-derived and metastasis-derived tissue were analyzed for sequences of SV40, JVC, BKV and EBV using endpoint PCR. In addition, the susceptibility of CRC cells to JCV and BKV was examined using a long-term cultivation approach of patient-individual cells in the presence of viruses.

Results

No virus-specific sequences could be detected in all specimens. Likewise, no morphological changes were observed and no evidence for viral infection or integration could be provided after long term CRC cell cultivation in presence of viral particles.

Conclusions

In summary, the presented data suggest that there is no direct correlation between tumorigenesis and viral load and consequently no evidence for a functional role of the DNA viruses included into this analysis in CRC development.
Impact of Early Chimerism Status on Clinical Outcome in Children with Acute Lymphoblastic Leukaemia after Haematopoietic Stem Cell Transplantation
Authors: Lejman Monika , Zaucha-Prażmo Agnieszka , Zawitkowska Joanna , Mroczkowska Aleksandra , Grabowski Dominik , Kowalczyk Jerzy Roman , Drabko Katarzyna 
Published: August 2019

Abstract
In this retrospective study we evaluated the clinical impact of very early chimerism after allogeneic haematopoietic stem cell transplantation (allo-HSCT) in children with acute lymphoblastic leukaemia (ALL). The study group included 38 boys and 18 girls. Very early chimerism was evaluated on days +7, +14, +21 and +28 after the transplant. Quantitative fluorescence polymerase chain reaction was used to evaluate chimerism. Overall survival (OS) and event-free survival (EFS) were 84% and 80% respectively. OS in group of 24 patients with complete donor chimerism on day +14 was 83% and it did not differ statistically compared to 32 patients with mixed chimerism on day +14 (OS was 84%). The donor type (matched unrelated) and sex (male; p = 0.02 on day +14, p = 0.01 on day +21, p = 0.02 on day 28), number of transplanted cell (above 4,47 x 106 kg; p = 0.036 on day +14) and serotherapy (without ATG; p = 0.05 on day +7, p = 0.016 on day +21, p = 0.023 on day 28) were statistically related to a high level of donor chimerism. Grades II-IV acute graft versus host disease were diagnosed in 23 patients, who presented level donor chimerism above 60% on day 7. The data presented in this study provides valuable insight to the analysis of very early chimerism in children with ALL treated with HSCT.
Icotinib is as efficacious as gefitinib for brain metastasis of EGFR mutated non-small-cell lung cancer
Authors: Liu Kejun , Jiang Guanming , Zhang Ailing , Li Zhuanghua , Jia Jun 
Published: August 2019

Abstract
Background: The prognosis of non-small-cell lung cancer (NSCLC) with brain metastases is very poor. Currently, therapeutic methods for this patient population include whole-brain radiation therapy (WBRT), surgery, radiosurgery and systemic treatment. Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) could be effective on cerebral metastases of mutated NSCLC. However, which EGFR-TKIs is more appropriate is still unknown.
Methods: A total of 43 patients were recruit in this study. Patients received either icotinib (125 mg, thrice a day) or geﬁtinib (250 mg, once a day) until disease progression or unacceptable toxicity. The primary end point of this study was PFS. The relationships between therapeutic arms and patients characteristics were performed using Pearson’s chi-square test or Fisher’s exact test. The differences in PFS among the two arms were analyzed using Kaplan-Meier curves and log rank tests.
Results: There was no significant difference of intracranial ORR (66.6% versus 59.1%, P =0.62) and DCR (85.7% versus 81.8%, P =0.73) between the two arms. The median intracranial PFS (iPFS) for icotinib and gefitinib arms were 8.4 months (95% CI, 5.4 to 11.3 months) and 10.6 months (95% CI, 6.3 to 14.8 months), respectively (P =0.17). Adverse events of the two study arms were generally mild. None of the patients experienced dose reduction of EGFR-TKIs.
Conclusions: Our study showed that icotinib and geﬁtinib had similar efficacy for brain metastasis of EGFR mutated NSCLC. Large randomized studies are suggested to further illuminate the effect of these two EGFR-TKIs on cerebral lesions of NSCLC.
Evaluation of the efficacy of casein phosphopeptide-amorphous calcium phosphate on remineralization of white spot lesions in vivo and in vitro: A systematic review and meta-analysis
Authors: Lin Xuandong , Ma Xueling , Zhong Tengfei , Xie Fangfang 
Published: August 2019

Abstract
Abstract
Background: This systematic review with meta-analyses sought to answer whether casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) provided a remineralizing benefit superior to that of nonintervention or placebo.
Methods: The Cochrane databases, PubMed, EmBase, and Ovid up to May, 2019, were scanned, with no restrictions. Study information extraction and methodological quality assessments were accomplished independently by two reviewers. The “Criteria for judging risk of bias in the ‘Risk of bias’ assessment tool” was used for methodological quality assessment. The continuous data was analyzed by mean difference (MD) or standardized mean difference (SMD) with a 95% confidence interval (CI). Review Manager 5.3 was used for statistical analysis. Outcome variables include quantitative light-induced fluorescence in vivo, average surface roughness and surface microhardness in vitro.
Results: There were significant differences in the quantitative light-induced fluorescence (SMD = −0.43, 95% CI: [−0.79, −0.07], P = 0.02), average surface roughness (SMD = −8.21, 95% CI: [−10.37, −6.04], P < 0.01), Vickers microhardness (SMD = 1.19, 95% CI: [0.72, 1.66], P < 0.01), and Knoop microhardness (SMD = 3.52, 95% CI: [2.68, 4.36], P < 0.01) between the CPP-ACP and control groups or baseline.
Conclusion: Within the limitations of this meta-analysis, CPP-ACP exhibited excellent remineralization effects evaluated in vivo and in vitro, indicating outstanding restoration of form, aesthetics, and function in treating white spot lesions.
Does Er,Cr:YSGG reduce the microleakage of restorations when used for cavity preparation: a systematic review and meta-analysis
Authors: Zhang Yali , Chen Wenfei , Zhang Jinrui , Li Yanhui 
Published: August 2019

Abstract
Background: As the member of erbium laser family, Erbium, Chromium: Yttrium Scandium Gallium Garnet (Er,Cr:YSGG) have obtained the approval for caries removal and cavity preparation by Food and Drug Administration (FDA). However, there are still disputes about the beneficial efficacy of Er,Cr:YSGG prepartions on microleakage. This is the first systematic review and meta-analysis to compare the microleakage of cavities prepared by Er,Cr:YSGG lasers with that by traditional burs. We also assess the effect of acid etching on the adhesive potential of self-etch and etch-and-rinse adhesives after laser preparation. Methods: An electronic search was performed in Pubmed, EBSCO, Embase, and the Cochrane Controlled Register of Trials (CENTRAL). Results: 357 articles were identified. Finally, 12 met the inclusion criteria, among which 11 were selected for meta-analysis. All included studies presented a moderate risk of bias. No significant difference was observed between the Er,Cr:YSGG and traditional bur groups in terms of the incident rate of microleakage. Self-etch adhesives in combination with prior acid etching shown less microleakage than those without acid etching in laser-prepared cavities. Conclusions: The current studies did not support the beneficial effects of Er,Cr:YSGG preparations on microleakage. And the additional acid etching is recommended to accompany with self-etching adhesives after Er,Cr:YSGG preparations. More high-quality researches are needed to draw a convincing conclusion in the future.
Container-aided Integrative QTL and RNA-seq Analysis of Collaborative Cross Mice Supports Distinct Sex-Oriented Molecular Modes of Response in Obesity
Authors: Binenbaum Ilona , Atamni Hanifa Abu-Toamih , Fotakis Georgios , Kontogianni Georgia , Koutsandreas Theodoros , Pilalis Eleftherios , Mott Richard , Himmelbauer Heinz , Iraqi Fuad A. , Chatziioannou Aristotelis 
Published: August 2019

Abstract
Background: The CC mouse population is a valuable resource to study the genetic basis of complex traits, such as obesity. Although the development of obesity is influenced by environmental factors, the underlying genetic mechanisms play a crucial role in the response to these factors. The interplay between the genetic background and the gene expression pattern can provide further insight into this response, but we lack robust and easily reproducible workflows to integrate genomic and transcriptomic information in the CC mouse population.
Results: We established an automated and reproducible integrative workﬂow to analyse complex traits in the CC mouse genetic reference panel at the genomic and transcriptomic levels. We implemented the analytical workﬂow to assess the underlying genetic mechanisms of host susceptibility to diet induced obesity and integrate these results with diet induced changes in the hepatic gene expression of susceptible and resistant mice. Hepatic gene expression diﬀers signiﬁcantly between obese and non-obese mice, with a signiﬁcant sex eﬀect, where male and female mice exhibit diﬀerent responses and coping mechanisms.
Conclusion: Integration of the data showed that diﬀerent genes but similar pathways are involved in the genetic susceptibility and disturbed in diet induced obesity. Genetic mechanisms underlying susceptibility to high-fat diet induced obesity diﬀer in female and male mice. The clear distinction we observe in the systemic response to the high-fat diet challenge and to obesity between male and female mice points to the need for further research into distinct sex-related mechanisms in metabolic disease.
The evolutionary analysis of PEBP Gene Family among Rosaceae tree species
Authors: Zhang Man , Li Ping , Yan Xiaolan , Wang Jia , Cheng Tangren , Zhang Qixiang 
Published: August 2019

Abstract
Phosphatidylethanolamine-binding proteins (PEBPs) are a common gene family found among animals, plants and microbes. Plant PEBP proteins play an important role in regulating flowering time, as well as seed and bud dormancy. PEBP proteins can be divided into three major clades: FLOWERING LOCUS T-like (FT-like), TERMINAL FLOWER1-like (TFL1-like), and MOTHER OF FT AND TFL1-like (MFT-like). Though PEBP family genes have been well studied in Arabidopsis and other model species, their functional role in perennial trees is not fully understood. To characterize the evolution of PEBP genes and their role in flowering control among Rosaceae species, we identified a total of 46 PEBP members in seven Rosaceae species. Sequence and gene structure analysis revealed highly conserved intron/exon distributions and featured motifs among Rosaceae PEBP proteins. Analysis of synonymous/nonsysnonymous substitution rates showed purifying selection constraining divergence within most lineages, while positive selection appears to have driven divergence of FT-like and TFL-like genes from the MFT clade. The expression of PEBP genes varied among different tissues indicating their functional divergence during gene family evolution. Furthermore, by employing a weighted gene co-expression network approach, we inferred a putative FT regulatory module essential for dormancy release and floral induction in P. mume. Our study sheds new light on the evolution of PEBP genes and their functional roles in controlling flowering time among Rosaceae tree species.
Size of pelvic bone metastasis as a significant prognostic factor for metastatic prostate cancer patients
Authors: Hayakawa Toyokazu , Tabata Ken-ichi , Tsumura Hideyasu , Kawakami Shogo , Katakura Takeo , Hashimoto Masatoshi , Watanabe Yusuke , Iwamura Masatsugu , Hasegawa Tomoyuki , Ishiyama Hiromichi 
Published: August 2019

Abstract
Background

To investigate the potential prognostic value of image analysis of pelvic bone metastasis in newly diagnosed prostate cancer patients.Methods

Data from 69 patients with both bone scintigraphy and pelvic CT images were selected for this analysis. Open source software (3D Slicer version 4.8.1.) was used for image analysis. Metastatic pelvic bone lesions were manually contoured, and radiomic features were extracted. As risk factors for overall survival (OS) and cause-specific survival (CSS), 105 radiomic features and clinical risk factors including age, initial prostate-specific antigen, Gleason score, TNM stage, lactate dehydrogenase (LDH), hemoglobin (Hb), alkaline phosphatase, extent of disease, visceral metastases, castration-resistant prostate cancer (CRPC), and number of systemic agents including abiraterone, enzalutamide, docetaxel and cabazitaxel which CRPC patients received were assessed by uni- and multivariate analyses.Results

Median follow-up was 41 months (range, 0-157 months). Three- and 5-year overall survival rates were 66.6% and 37.9%, respectively. Three- and 5-year cause-specific survival rates were 69.4% and 43.5%, respectively. After multivariate analysis, LDH, Hb, and “maximum 2D diameter” defined as maximum tumor size in the axial plane were detected as risk factors for OS. Gleason sum, LDH, and maximum 2D diameter were detected as risk factors for CSS.Conclusion

Maximum 2D diameter of pelvic bone metastasis was detected as a significant prognostic factor for metastatic prostate cancer patients.
The diagnostic and prognostic value of heme oxygenase 1 in sepsis-induced acute kidney injury: a cross-sectional observational study
Authors: Xia Shilin , Zhang Meishuai , Liu Han , Wu Nannan , Chen Huiqing , Li Nan 
Published: August 2019

Abstract
Background
Sepsis patients suffer from severe inflammatory and poor prognosis. Local inflammation resulted from sepsis is found to trigger organ injury, such as acute kidney injury (AKI). We conducted a cross-sectional observational study to examine the diagnostic and prognostic role of serum heme oxygenase 1 (HO-1) on sepsis-induced AKI.

Methods
The 83 enrolled patients were initially divided into two groups with no AKI (NAKI) and sepsis-induced AKI group (SAKI) based on whether had AKI. Then the patients were concretely divided into four groups: septic shock and AKI group (SS+AKI group), sepsis-induced AKI group (S+AKI group), septic shock group (SS group), and sepsis group (S group. The venous blood was sampled within 24 hours after diagnosis of sepsis. We detected the serum HO-1 and laboratory indicators by enzyme-linked immunosorbent assay. The 28-day survival status was observed between the HO-1 high- and low-level patients grouped by the median of HO-1 concentration 41.33U/L.

Results
We found that there were statistically significant results of SOFA score and admission time between SAKI and NAKI group (p<0.05). The level of HO-1 in SAKI group was obviously elevated as compared with NAKI group (p<0.05). It was remarkable to show that HO-1 level was remarkably higher in SS+AKI group than that in other three groups (p<0.05). In All groups, HO-1 positively correlates with SOFA score, Scr, Hb, APTT, Urea, and TnI (p<0.05). In SS+AKI group, HO-1 was positively associated with SOFA score, Scr, AKI stage, γ-GT, and FDP (p<0.05). In S+AKI, HO-1 level was positively related to the level of Scr, AKI stage, and ALP (p<0.05). In SS group, SOFA score was in negative correlation to HO-1 (p<0.05). The AUC of HO-1 and serum creatinine was 0.824 (95% CI: 0.703-0.944) and 0.778 (95% CI: 0.658-0.919) separately. The AUC of combined with HO-1 and serum creatinine was 0.864 (95% CI: 0.761-0.968). The survival analysis showed that sepsis patients with high HO-1 level had a higher mortality rate compared with patients with low HO-1 expression.

Conclusions
The findings from this study make contributions to clinical value of HO-1, suggesting that HO-1 plays a diagnostic and prognostic role in sepsis-induced AKI.
Salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry
Authors: Ganesan Saibaba , Durairaj Rajesh , Subramanian Muthukumar , Ganesan Sathiyanarayanan , Parasuraman Padmanabhan , Balazs Gulyas , Abdulkader Akbarsha Mohammad , Govindaraju Archunan 
Published: August 2019

Abstract
Background Human saliva contains several biomolecules, especially proteins, some of which have been found to serve as biomarkers of different physiological statuses and/or pathological conditions. Saliva is a much superior biological material for investigation over the other body fluids. Several salivary proteins are present in minute quantities whereupon there is possibility to express the hormone receptors, stress proteins or antimicrobial peptides at subtle to major variations which can reveal the physiological and/or pathological statuses. Ovulation is such a critical physiological process that its non-invasive prediction has several advantages. It has been postulated that saliva, with one or more of biomolecules in it such as proteins, amino acids, antioxidants, antimicrobial peptides, and such others would effectively serve as non-invasive predictor(s)/detector(s) of ovulation.Methods Samples were collected from women volunteers and the procedure adopted was approved by the Institutional Ethical Committee (DM/2014/101/38), Bharathidasan University. The saliva samples were collected between 8.00 to 9.00 AM from 30 healthy female volunteers (age, mean = 24, range = 19 - 30), with prior written consent. The protein expression pattern during different phases of menstrual cycle was analyzed using HR-LC-MS/MS and MALD TOF/TOF.Results The functional annotation of salivary proteins identified therein revealed that the proteins get assigned to the class of “extracellular proteins” which are concerned with regulatory functions. As many as 530 proteins showed up in the saliva during ovulation phase whereas there were only 251 proteins during post-ovulation phase. The protein expression was also analyzed using 2D gel electrophoresis and it was found that the salivary proteins focused at pH range 4-7 and molecular weight around 97-14 kDa. The unique/differentially expressed protein spots appeared during ovulation phase was identified by MALDI-TOF analysis.Conclusions According to spot analysis, Cystatin-S, Prolactin-inducible protein, Cystatin-A, Cystatin-SN, BPI fold-containing family A member 2, Alpha-tubulin N-acetyltransferase 1, and Carbonic anhydrase 6 were highly expressed during ovulation phase. These proteins could serve as an indicator(s) for ovulation after extensive validation studies.