bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating enzyme evolution and high-throughput screening for efficient biosynthesis of l-DOPA
Authors: Zeng Weizhu , Xu Bingbing , Du Guocheng , Chen Jian , Zhou Jingwen 
Published: September 2019

Abstract
l-DOPA is a key pharmaceutical agent for treating Parkinson’s, and market demand has exploded due to the aging population. There are several challenges associated with the chemical synthesis of l-DOPA, including complicated operation, harsh conditions, and serious pollution. A biocatalysis route for l-DOPA production is promising, especially via a route catalyzed by tyrosine phenol lyase (TPL). In this study, using TPL derived from Erwinia herbicola (Eh-TPL), a mutant Eh-TPL was obtained by integrating enzyme evolution and high-throughput screening methods. l-DOPA production using recombinant Escherichia coli BL21 (DE3) cells harbouring mutant Eh-TPL was enhanced by 36.5% in shake flasks, and the temperature range and alkali resistance of the Eh-TPL mutant were promoted. Sequence analysis revealed two mutated amino acids in the mutant (S20C and N161S), which reduced the length of a hydrogen bond and generated new hydrogen bonds. Using a fed-batch mode for whole-cell catalysis in a 5 L bioreactor, the titre of l-DOPA reached 69.1 g L−1 with high productivity of 11.52 g L−1 h−1, demonstrating the great potential of Eh-TPL variants for industrial production of l-DOPA.
Longitudinal change in physical functioning and dropout due to death among the oldest old: a comparison of three methods of analysis
Authors: Raitanen Jani , Stenholm Sari , Tiainen Kristina , Jylhä Marja , Nevalainen Jaakko 
Published: September 2019

Abstract
Longitudinal studies examining changes in physical functioning with advancing age among very old people are plagued by high death rates, which can lead to biased estimates. This study was conducted to analyse changes in physical functioning among the oldest old with three distinct methods which differ in how they handle dropout due to death. The sample consisted of 3992 persons aged 90 or over in the Vitality 90+ Study who were followed up on average for 2.5 years (range 0–13 years). A generalized estimating equation (GEE) with independent ‘working’ correlation, a linear mixed-effects (LME) model and a joint model consisting of longitudinal and survival submodels were used to estimate the effect of age on physical functioning over 13 years of follow-up. We observed significant age-related decline in physical functioning, which furthermore accelerated significantly with age. The average rate of decline differed markedly between the models: the GEE-based estimate for linear decline among survivors was about one-third of the average individual decline in the joint model and half the decline indicated by the LME model. In conclusion, the three methods yield substantially different views on decline in physical functioning: the GEE model may be useful for considering the effect of intervention measures on the outcome among living people, whereas the LME model is biased regarding studying outcomes associated with death. The joint model may be valuable for predicting the future characteristics of the oldest old and planning elderly care as life expectancy continues gradually to rise.
Media studies in the UK
Authors: Golding P. 
Published: September 2019

Abstract
This article outlines the growth and character of media and communication studies in the UK. It sets out the history and development of the field, and explains its twin origins in both humanities and social sciences contexts. The article also presents some descriptive data about the scale and nature of teaching and research in the field in UK higher education, and explains the evolution of relevant subject associations. The public, political and professional reception of and responsse to the field are described, and the continuing debates about its value and salience examined.
Psychological and Sexual Consequences of Psoriasis Vulgaris on Patients and Their Partners
Authors: Alariny Ali Fouad , Farid Carmen Ibrahim , Elweshahi Heba Mahmoud , Abbood Sahar Salim 
Published: September 2019

Abstract

Introduction
Psoriasis is a chronic, multisystem, inflammatory, disfiguring disease with a high negative impact on quality of life due to comorbid psychological, organic, and sexual consequences.

Aim
To evaluate the psychopathological and sexual aspects of psoriasis vulgaris in patients and their partners compared to healthy controls.

Methods
In the current comparative cross-sectional study, the sample included 220 psoriasis vulgaris patients (110 males and 110 females), their consenting partners, and 220 age- and sex-matched healthy controls. All participants were evaluated for depression and anxiety using the Hospital Anxiety and Depression Scale and for low self-esteem using the Rosenberg's Self-Esteem Questionnaire. Sexual function was assessed in females using the Female Sexual Function Index questionnaire and in males using the International Index of Erectile Function and its abbreviated 5-item version. For male patients suffering from erectile dysfunction, hormonal assessment and penile duplex ultrasound were added.

Main Outcome Measure
The main outcome measures were frequency of depression, anxiety, low self-esteem, and sexual dysfunction in psoriasis vulgaris patients, partners, and controls; the domains of sexual function affected in the studied groups; and the etiology of erectile dysfunction in affected psoriatic males.

Results
Frequency of depression, anxiety, and low self-esteem were significantly higher in psoriasis patients of both sexes compared with controls. Sexual dysfunction followed the same trend of being significantly higher in cases when compared to controls. Females had a significantly higher frequency of sexual dysfunction than males. Sexual dysfunction in both sexes involved multiple domains of the sexual response cycle, with depression and genital affection by psoriasis being risk factors in both sexes and disease severity being an additional risk factor in females. Erectile dysfunction was mainly psychogenic in origin in male patients.

Clinical Implications
Assessment of psychopathological and sexual comorbidities in psoriasis patients and partners should be an integral part of the management plan and should be added to the guidelines of the disease.

Strength & Limitations
This study is of sufficient power to draw solid conclusions. Assessment of the partners and determination of the sexual domains affected by the disease and nature of erectile dysfunction in males are points of novelty. The cross-sectional design is a limitation of the study.

Conclusion
Psoriatic patients of both sexes and their partners suffer from a high burden of psychopathological and sexual consequences that are related to disease distribution and severity.
Dyspareunia Related to GSM: Association of Total Vaginal Thickness via Transabdominal Ultrasound
Authors: Balica Adrian C. , Cooper Alexandria M. , McKevitt Mariah K. , Schertz Katherine , Wald-Spielman Daniella , Egan Susan , Bachmann Gloria A. 
Published: September 2019

Abstract

Introduction
It has previously been suggested in the literature that ultrasound measurement of total vaginal wall thickness (TVT) differs significantly between pre- and postmenopausal women, indicating that it may be a useful and noninvasive objective assessment to correlate the degree of vaginal atrophy to patient-reported symptoms.

Aim
The purpose of this cross-sectional pilot study was to determine whether TVT in postmenopausal women, as measured with transabdominal ultrasound, is associated with patient-reported dyspareunia and symptoms related to genitourinary symptomatology.

Methods
Postmenopausal women presenting for pelvic ultrasound had TVT and total mucosal thickness (TMT) measured via transabdominal ultrasound. A questionnaire also was administered assessing menopausal status, relevant medical history, and self-report of dyspareunia and other symptoms related to the genitourinary syndrome of menopause (GSM). This questionnaire was derived from the Vulvovaginal Symptom Questionnaire, which has been validated in the literature.

Main Outcome Measure
The main outcome measures included the average TVT and TMT for postmenopausal women reporting any symptom of GSM and average TVT and TMT of women reporting no symptoms of GSM.

Results
Data from 44 postmenopausal women showed no significant association between transabdominal ultrasound-measured TVT or TMT and patient report of dyspareunia or other genitourinary symptoms. Data were stratified by individual GSM symptoms, sexual symptoms as an aggregate, and individual sexual symptoms. Neither of these subgroups showed a statistically significant difference in TVT or TMT between symptomatic and asymptomatic women.

Clinical Implications
Although no statistically significant data were derived from this study, we propose that future studies investigating the longitudinal relationship between TVT and GSM symptomatology may show an association between total vaginal thickness measurement change over time as determined by ultrasound with the presence of patient-reported dyspareunia and other GSM symptoms.

Strengths & Limitations
This study is limited by its small sample size as well as the patient population, which was restricted to postmenopausal women with a clinical indication for ultrasound. A major strength of this investigation is that it is the first study to look at the relationship between sexual pain and other GSM symptoms and TVT using transabdominal ultrasound, which is a readily available, non-invasive tool in most clinical settings.

Conclusion
Based on the results of this small pilot study, transabdominal pelvic ultrasound cannot be used at this time to objectively quantify the presence of sexual pain or other GSM symptoms; however, future studies should continue to investigate the longitudinal relationship between these 2 variables.
Beyond the Post-Sovereign State?: The Past, Present, and Future of Constitutional Pluralism
Authors: WILKINSON Michael 
Published: September 2019

AbstractConstitutional pluralism is a theory for the post-sovereign European state. This only makes sense historically, emerging out of postwar European reconstruction through the repression of popular sovereignty and restraining of democracy, including through the project of European integration. It became unsettled at Maastricht and evolved from a series of irritants into a full-blown crisis in the recent decade, with sovereignty claims returning both from the bottom-up and the top-down, to the extent that we can legitimately ask whether we are now moving ‘beyond the post-sovereign state’? Constitutional pluralist literature fails to capture this in that evades material issues of democracy and political economy.
Sheared free-surface flow over three-dimensional obstructions of finite amplitude
Authors: Akselsen Andreas H. , Ellingsen Simen Å. 
Published: September 2019

When shallow water flows over uneven bathymetry, the water surface is modulated. This type of problem has been revisited numerous times since it was first studied by Lord Kelvin in 1886. Our study analytically examines currents whose unperturbed velocity profile$U(z)$follows a power law $z^{q}$, flowing over a three-dimensional uneven bed. This particular form of $U$, which can model a miscellany of realistic flows, allows explicit analytical solutions. Arbitrary bed shapes can readily be imposed via Fourier’s theorem provided their steepness is moderate. Three-dimensional vorticity–bathymetry interaction effects are evident when the flow makes an oblique angle with a sinusoidally corrugated bed. Streamlines are found to twist and the fluid particle drift is redirected away from the direction of the unperturbed current. Furthermore, a perturbation technique is developed which satisfies the bottom boundary condition to arbitrary order also for large-amplitude obstructions which penetrate well into the current profile. This introduces higher-order harmonics of the bathymetry amplitude. States of resonance for first- and higher-order harmonics are readily calculated. Although the method is theoretically restricted to bathymetries of moderate inclination, a wide variety of steeper obstructions are satisfactorily represented by the method, even provoking occurrences of recirculation. All expressions are analytically explicit and sequential fast Fourier transformations ensure quick and easy computation for arbitrary three-dimensional bathymetries. A method for separating near and far fields ensures computational convergence under the appropriate radiation condition.
Cécile Morette and the Les Houches summer school for theoretical physics; or, how Girl Scouts, the 1944 Caen bombing and a marriage proposal helped rebuild French physics (1951–1972)
Authors: VERSCHUEREN PIERRE 
Published: September 2019

AbstractThe aftermath of the Second World War represented a major turning point in the history of French and European physical sciences. The physicist's profession was profoundly restructured, and in this transition the role of internationalism changed tremendously. Transnational circulation became a major part of research training. This article examines the conditions of possibility for this transformation, by focusing on the case of the summer school for theoretical physics created in 1951 by the young Cécile Morette (1922–2017), just in front of Mont Blanc, at Les Houches. First I show that ultimately it was only thanks to extremely specific and intertwined social positions and dispositions, in terms of class and gender (derived from her socialization as an expecteddame de la bourgeoisie), and through the interactions between such social attributes and a dramatic life event, that Morette managed to gather a network diverse, powerful and transnational enough to create this institution. Then, following the first years of this school, I show how it became an international model, paving the way to new articulations between the local, the national and the global scales, even beyond the Cold War oppositions.
Physical activity and Baltic Sea diet are interactively related to higher life satisfaction in community-living older Finnish women: OSTPRE-FPS study
Authors: Isanejad Masoud , Koivumaa-Honkanen Heli , Ruiz de Santiago Diana , Honkanen Risto , Rikkonen Toni , Sirola Joonas , Kröger Heikki , Erkkilä Arja T. 
Published: September 2019

AbstractA healthful diet and sufficient physical activity (PA) are related to several health outcomes. However, there is a paucity of data on the association of PA and dietary pattern with life satisfaction (LS) in the older adults aged ≥65. The present study investigated the independent and combined association of PA and Baltic Sea diet (BSD) score with LS in older Finnish women. Subjects were 554 women aged 65–72 years from the Osteoporosis Risk Factor and Prevention – Fracture Prevention Study. Women reported the hours and type of PA and lifestyle factors via questionnaires and dietary intake using the 3-d food record. Adequate PA was considered according to WHO recommendation: PA = 0, 0 < PA < 2·5 and ≥2·5 h/week. BSD score was categorised as <13 or ≥13 based on the median score. LS was self-reported using LS scale with four items on current ‘interest’, ‘happiness in life’, ‘ease of living’ and ‘feelings of loneliness’ (range: 4–20, lower score representing higher satisfaction). After adjusting for the confounders, PA was statistically significantly associated with lower LS score (β coefficient = −0·207, P = 0·001), where women with PA ≥ 2·5 h/week had the lowest LS score followed by women with 0 < PA < 2·5 and PA = 0 (Pfor trend = 0·020). Association between BSD and LS was NS. Only among women with BSD score ≥ 13, but not BSD < 13, PA ≥ 2·5 h/week was statistically significantly associated with lower LS score (mean = 9·3), followed by 0 < PA < 2·5 (mean = 9·9) and PA = 0 groups (mean = 11·8) (Pfor trend = 0·033). In conclusion, adequate PA according to WHO recommendation independently and in combination with higher BSD score may be associated with higher LS in older women.
A HISTORICAL EXAMINATION OF VICARIOUS LIABILITY: A “VERITABLE UPAS TREE”?
Authors: Swain Warren 
Published: September 2019

AbstractVicarious liability was, and it remains, curiously unsatisfactory. After a period of stability from the Middle Ages into the early modern period in the late seventeenth into the early eighteenth century, the existing law of vicarious liability began to be challenged. The mid-nineteenth century saw another reappraisal coinciding with the rise of notions of fault. The period that follows, from the late nineteenth century until after the Second World War period has not attracted much comment. One key debate in this period and earlier which provides a useful lens to examine the doctrine was whether vicarious liability should be properly characterised as a master's or servant's tort theory. The history of the doctrine during this period goes some way to explaining why the modern law remains incoherent.
Autistic spectrum disorder symptoms in children and adolescents with attention-deficit/hyperactivity disorder: a meta-analytical review
Authors: Hollingdale Jack , Woodhouse Emma , Young Susan , Fridman Adie , Mandy Will 
Published: September 2019

AbstractBackgroundResearch identifies highly variable prevalence estimates for autism spectrum disorder (ASD) in children and adolescents with attention deficit hyperactivity disorder (ADHD), particularly between community and clinical samples, warranting quantitative meta-analyses to investigate the true prevalence of ASD in children and adolescents with ADHD.MethodsStudies were identified through a systematic literature search of PsycINFO, MEDLINE and Web of Science through January 2018. Twenty-two publications met inclusion criteria (totalN= 61 985). Two random effects meta-analyses were conducted: (1) to identify the proportion of children and adolescents with ADHD that met criteria for ASD; and (2) to compare the severity of dimensionally-measured ASD symptomology in children and adolescents with and without ADHD.ResultsThe overall pooled effect for children and adolescents with ADHD who met threshold for ASD was 21%. There was no significant difference between community samples (19%) and clinical samples (24%) or between US studiesv.those from other countries. Children and adolescents with ADHD had substantially more dimensionally-measured ASD traits compared with those who did not have ADHD (d= 1.23).ConclusionThe findings provide further evidence that ADHD and ASD are associated in nature. Clinical and research implications are discussed.
The association of striatal volume and positive schizotypy in healthy subjects: intelligence as a moderating factor
Authors: Meller Tina , Ettinger Ulrich , Grant Phillip , Nenadić Igor 
Published: September 2019

AbstractBackgroundSchizotypy, a putative schizophrenia endophenotype, has been associated with brain-structural variations partly overlapping with those in psychotic disorders. Variations in precuneus structure have been repeatedly reported, whereas the involvement of fronto-striatal networks – as in schizophrenia – is less clear. While shared genetic architecture is thought to increase vulnerability to environmental insults, beneficial factors like general intelligence might buffer their effect.MethodsTo further investigate the role of fronto-striatal networks in schizotypy, we examined the relationship of voxel- and surface-based brain morphometry and a measure of schizotypal traits (Schizotypal Personality Questionnaire, with subscores Cognitive-Perceptual, Interpersonal, Disorganised) in 115 healthy participants [54 female, mean age (s.d.) = 27.57(8.02)]. We tested intelligence (MWT-B) as a potential moderator.ResultsWe found a positive association of SPQ Cognitive-Perceptual with putamen volume (p = 0.040, FWE peak level-corrected), moderated by intelligence: with increasing IQ, the correlation of SPQ Cognitive-Perceptual and striatal volume decreased (p = 0.022). SPQ Disorganised was positively correlated with precentral volume (p = 0.013, FWE peak level-corrected). In an exploratory analysis (p < 0.001, uncorrected), SPQ total score was positively associated with gyrification in the precuneus and postcentral gyrus, and SPQ Disorganised was negatively associated with gyrification in the inferior frontal gyrus.ConclusionsOur findings support the role of fronto-striatal networks for schizotypal features in healthy individuals, and suggest that these are influenced by buffering factors like intelligence. We conclude that protective factors, like general cognitive capacity, might attenuate the psychosis risk associated with schizotypy. These results endorse the idea of a continuous nature of schizotypy, mirroring similar findings in schizophrenia.
Satisfaction and restoration of the world
Authors: PAWL FAITH GLAVEY 
Published: September 2019

AbstractEleonore Stump's Atonement locates the problem to be solved by Christ's passion and death in human proneness to sin, and in human guilt and shame. In addition to offering a way to fix what is broken in a person's psyche, Stump's understanding of satisfaction allows her to show how Christ's passion and death restore relationship between humans and God and between humans and what they have wrecked by sin. I argue that her story should be extended to show how the Atonement can restore the damaged relationships humans have with the rest of creation – not just with fellow humans.
What Is a Theory of Normative Concepts For?
Authors: Chrisman Matthew 
Published: September 2019

AbstractThis paper compares and contrasts two recent approaches to the theory of normative concepts with each other and with more traditional theories in metaethics, in order to highlight several different projects one could be engaged in when developing a theory of normative concepts. The two accounts derive from Millgram, The Great Endarkenment (Oxford University Press, 2015) and Chrisman The Meaning of ‘Ought’ (Oxford University Press, 2016). These accounts share in rejecting traditional attempts to explain what ‘ought’ is about or expresses. Instead these accounts treat ‘ought’ as a quantificational word. However, the nature and range of the quantification are importantly different in the two accounts, which impacts on the ways in which the accounts integrate with the various projects one could be engaged in when developing a theory of normative concepts.
Expressivism and Crossed Disagreements
Authors: Osorio Javier , Villanueva Neftali 
Published: September 2019

AbstractThe purpose of this paper is to explore the connection between expressivism and disagreement. More in particular, the aim is to defend that one of the desiderata that can be derived from the study of disagreement, the explanation of ‘crossed disagreements’, can only be accommodated within a semantic theory that respects, at the meta-semantic level, certain expressivistic restrictions. We will compare contemporary dynamic expressivism with three different varieties of contextualist strategies to accommodate the specificities of evaluative language –indexical contextualism – truth-conditional pragmatics –, pragmatic strategies using implicatures, and presuppositional accounts. Our conclusion will be that certain assumptions of expressivism are necessary in order to provide a semantic account of evaluative uses of language that can allow us to detect and prevent crossed disagreements.