bluuebunny/crossref_metadata_2025_split huggingface dataset:
Herbal formula xuling-jiangu improves bone metabolic balance in rats with ovariectomy-induced osteoporosis via the gut-bone axis
Authors: Chen Juan , Ng Szetuen , Xu Pengchao , Chen Sainan , Li Shengqiang , Chen Xuan , Xie Lihua , Ge Jirong 
Published: November 2024

IntroductionThe XuLing JianGu recipe (XLJGR) is an empirical traditional Chinese medicine formula used for the treatment of osteoporosis. This study aims to explore the effects of XLJGR on the intestinal microbiota composition and endogenous metabolites in ovariectomized (OVX) rats.MethodsAn OVX rat model was established to evaluate the intervention effects of XLJGR. The measured indicators included bone density, serum bone metabolism markers, and an analysis of the types and abundances of intestinal microbiota, along with changes in endogenous metabolites. Additionally, MC3T3-E1 cells were used to validate the differential metabolites.ResultsXLJGR significantly reduced the abundance of Bacteroides, Butyricicoccus, and other bacterial strains in the gut. KEGG metabolic pathway enrichment analysis showed that XLJGR intervention led to notable changes in pathways such as peptidoglycan biosynthesis, carbapenem biosynthesis, and vancomycin resistance. Moreover, XLJGR significantly upregulated key intestinal microbiota metabolites, including gabapentin(GAB), camphoric acid(CAA), and nonanedioic acid(AZA), thereby promoting the proliferation and osteogenic differentiation of MC3T3-E1 cells.DiscussionThis study highlights the potential biomedical applications of XLJGR in promoting bone health by positively affecting intestinal microbiota and metabolic characteristics. These findings suggest that XLJGR may serve as a viable alternative in the treatment of osteoporosis, warranting further exploration of its therapeutic mechanisms and clinical applications.
A sex-dependent role of Kv1.3 channels from macrophages in metabolic syndrome
Authors: Peraza Diego A. , Benito-Salamanca Lucía , Moreno-Estar Sara , Alonso Esperanza , López-López José R. , Pérez-Garcia M. Teresa , Cidad Pilar 
Published: November 2024

IntroductionCoronary artery disease (CAD) is the foremost single cause of mortality and disability globally. Patients with type 2 diabetes (T2DM) have a higher incidence of CAD, and poorer prognosis. The low-grade inflammation associated to T2DM contributes to increased morbidity and worst outcomes after revascularization. Inflammatory signaling in the vasculature supports endothelial dysfunction, leukocyte infiltration, and macrophage activation to a metabolic disease (MMe) specific phenotype, which could contribute to the metabolic disorders and ascular damage in T2DM. We have previously found that Kv1.3 blockers inhibit the development of intimal hyperplasia, thereby preventing restenosis. This inhibition was enhanced in a mouse model of T2DM, where systemic Kv1.3 blockers administration also improve metabolic dysfunction by acting on unidentified cellular targets other than vascular smooth muscle. Here we characterize the MMe phenotype in our T2DM model with a focus on macrophage Kv1.3 channels, to explore their contribution to vascular disease and their potential role as targets to ameliorate T2DM vascular risk.Methods and ResultsMale and female BPH mice fed on high-fat diet (HFD) develop metabolic syndrome (MetS) and T2DM. mRNA levels of several K+ channels (KV1.3, KCa3.1, Kir2.1) and macrophage markers (TNFα, NOS2, CD36) were analyzed. The MMe phenotype associated with increased CD36 expression. Channel-specific fingerprinting highlights a gender-specific increase of KV1.3 mRNA fold change in LPS stimulated macrophages from HFD compared to standard diet (SD). KV1.3 functional expression was also significantly increased after LPS stimulation in female HFD macrophages compared to SD. Functional studies showed that macrophage's KV1.3 channels of BPH female mice did not contribute to phagocytosis or metabolic profile but were relevant in cell migration rate.ConclusionAltogether, our data suggest that by inhibiting macrophage infiltration, Kv1.3 blockers could contribute to disrupt the vicious cycle of inflammation and insulin resistance, offering a novel approach to prevent MetS, T2DM and its associated cardiovascular complications in females.
Coming out of isolation: impacts of COVID-19 on physical activity, diet, mental well-being, and sleep over time
Authors: Billings Joel , Kwesell Allison , Cosby Stephanie , Lin Shuyang 
Published: November 2024

IntroductionThe COVID-19 pandemic lockdown significantly disrupted daily routines and impacted physical activity, diet, mental well-being, and sleep. This mixed-methods study investigates these changes over three periods—pre-pandemic, pandemic onset, and one-year post-onset—to understand their causes and inform public health policy for improved resilience during future crises.MethodsA mixed-methods study was conducted with 34 US participants who completed open-ended qualitative questions and quantitative assessments in May 2020 and May 2021. Participants were recruited via social media from 10 states with high COVID-19 cases. Quantitative data categorized health changes (positive, negative, or no change), while qualitative data were analyzed thematically to explore reasons for these changes and uncover interrelationships among health behaviors.ResultsQuantitative results showed that, during the pandemic onset, most participants experienced negative changes in diet, mental well-being, and sleep, while physical activity varied. By one-year post-onset, improvements were noted across all health pillars, with mental well-being and sleep showing the most significant positive changes. Thematic analysis of qualitative data revealed stress, anxiety, and personal motivations as key drivers of health behaviors. Participants’ narratives provided a deeper understanding of these shifts that a Likert-style survey alone could not capture, highlighting the interconnectedness of health pillars.DiscussionThe findings demonstrate the importance of addressing mental well-being to improve overall health resilience. Public health interventions should prioritize mental health and consider the interrelated nature of health behaviors. The mixed-methods approach combined quantitative trends with qualitative insights, offering a comprehensive understanding of why health changes occurred, providing actionable guidance for future public health policy.
Gender differences in the effects of urban environment on nighttime exercise behaviours: a qualitative study
Authors: Su Yuqin , Pan Xiaoli , Li Yike , Li Guanchong , Zhang Guodong 
Published: November 2024

ObjectiveWith the acceleration of urbanization, nighttime exercise behaviours have rapidly emerged. Existing research indicates a correlation between urban environments and physical activity; however, studies focusing specifically on nighttime are still insufficient, particularly regarding gender differences. This study aims to identify the key factors in urban environments that influence residents’ nighttime exercise behaviours and to explore the gender differences within these influences.MethodsPurposeful sampling was employed to conduct semi-structured interviews with 30 residents who regularly engage in nighttime exercise. All transcribed interviews were analyzed using Colaizzi’s phenomenological data analysis method.ResultsThe findings revealed that physical environment and environmental perception are the two primary factors influencing nighttime exercise behaviour. These factors are further divided into 10 specific sub-themes: lighting, green spaces, site facilities and layout, traffic coherence, entertainment facilities, smart sports equipment, sense of safety, convenience, pleasure, and sense of belonging.ConclusionFemales demonstrate a heightened sensitivity to the perception of the physical environment, placing greater emphasis on the feelings and experiences it provides. Males, on the other hand, focus more on the direct impact of the physical environment, such as its specific effects on exercise performance. Future urban planning and public policy should give greater consideration to gender differences in the use of urban exercise facilities, ensuring that nighttime exercise environments meet the needs of residents of different genders. This approach will contribute to enhancing overall community vitality and improving residents’ health.
Chasing among older-aged gamblers: the role of mentalizing and psychological distress
Authors: Ciccarelli Maria , Pizzini Barbara , Cosenza Marina , D’Olimpio Francesca , Griffiths Mark D. , Nigro Giovanna 
Published: November 2024

BackgroundDespite the increasing proportion of older-aged individuals suffering from problematic gambling, research on gambling among this specific age cohort is still in its infancy. Chasing is a pathognomonic feature of disordered gambling and is considered one of the key risk factors in the transition from recreational to disordered gambling. Despite the increased research on chasing over the past decade, no previous study has ever examined the psychological determinants of chasing behavior among old-aged gamblers. Given the importance of chasing in facilitating and maintaining problem gambling, and the paucity of research examining gambling among older individuals, the present study is the first to empirically investigate the joint role of chasing behavior, negative affectivity, and mentalizing among older-aged gamblers.MethodsThe sample comprised 116 older-aged gamblers who were administered the South Oaks Gambling Screen (SOGS), the Depression Anxiety Stress Scale (DASS-21) and the Reflective Functioning Questionnaire (RFQ-8) to assess gambling severity, psychological distress, and mentalizing, respectively. Participants also performed the ChasIT, a computerized task that assesses chasing behavior, in which participants were randomly assigned to three different experimental conditions: loss, control, and win.ResultsNo effect of the experimental conditions of ChasIT on chasing behavior was observed. Regression analyses indicated that heightened levels of gambling severity and lower levels of certainty about mental states (i.e., hypermentalizing) predicted both the decision to chase and chasing frequency. Along with problem gambling and hypermentalizing, chasing frequency was also predicted by high levels of depression.ConclusionsThe present study demonstrated the association between disordered gambling, depression, and hypermentalizing in chasing behavior among older-aged gamblers. The findings make an important contribution to providing insight regarding variables that are associated with chasing among older-aged gamblers, one of the least represented populations of gamblers in the literature. The results suggest that specific training on mentalizing abilities could help gamblers to reflect on their own behaviors in terms of mental states, rather than following the impulse to gamble in order to ameliorate poor mood.
The influence of policy advocacy and education on medical staff’s adaptation to diagnosis related groups payment reform in China: an analysis of the mediating effect of policy cognition
Authors: Ni Zhi-Ying , Zhang Bo-Kai , Song Lei , Zang Zhao-Yan , Yu Hong 
Published: November 2024

IntroductionIn recent years, China has been carrying out the Diagnosis Related Groups (DRGs) payment reform, which has an impact not only on payment methods and medical expenses, but also on the behaviors of medical staff. Some of these behaviors are unexpected by policymakers, such as turning away critically ill patients, disaggregating hospitalization costs, setting up disease groups with higher points, and so on. This phenomenon attracted the attention of some scholars, who put forward a few positive intervention measures, mainly including policy advocacy and system improvement. The scholars believed that the former was more feasible. However, there is a lack of research on the effects and influencing processes of these interventions. Therefore, this study aims to explore the influence of policy advocacy and education on medical staff’s adaptation to DRGs payment reform in China and the role of policy cognition in this process, in order to provide experiences for the smooth implementation and sustainable development of DRGs payment system.MethodsA questionnaire survey was conducted among 650 medical staff in five large general hospitals in Zhejiang Province, China, to understand their participation and feedback on policy advocacy and education, their adaptation to the current DRGs payment reform, and their cognition of relevant policies. After descriptive statistical analysis, partial correlation analysis, multiple linear regression models and bias correction Bootstrap sampling method were used to analyze the mediating effect of policy cognition factors.ResultsAll respondents had participated in organized collective policy advocacy and education activities in the past year, but the degree of satisfaction and recognition was not very high. 59.5 percent said their adaptation to the DRGs payment reform was average. Nearly half did not regularly pay attention to and participate in the management of the medical costs of patients with DRGs through compliance. And they had a low understanding of the specific rules of DRGs payment and did not form a high policy identity. The mediating effect values of policy cognition were 0.148, 0.152, 0.108, and 0.057, respectively, when the frequency and quality of policy advocacy and education influenced medical staff’s adaptive perception and adaptive behaviors.DiscussionThe organized collective policy advocacy and education can promote medical staff’s adaptation to DRGs payment reform by improving their policy cognition, and the action paths are diverse. Policymakers and hospital managers need pay attention to this phenomenon, and formulate demand-centered, value-oriented whole-process advocacy and education strategies while constantly improving the DRGs payment system. All of these provided a basis for further research and practice of positive intervention in DRGs payment reform.
Comparing the economic burden of disease by physical activity levels among persons with and without disabilities in the Republic of Korea
Authors: Lee SeungCheor , Oh Chang-Mo , Jung Young-il , Park So-Youn , Oh In-Hwan 
Published: November 2024

IntroductionThe World Health Organization recommends at least 150 min of physical activity per week for persons with and without disabilities. This study compared the differences in the economic burden of diseases between persons with and without disabilities based on their physical activity. What is known in the field is that physical activity is beneficial to health, but there is a disparity between persons without disabilities and persons with disabilities, and our research aims to increase physical activity among persons with disabilities.MethodsEconomic burden of disease includes service costs such as outpatient, hospitalization, and pharmaceutical expenses for disease treatment, and persons with disabilities are those who have received a disability classification legally registered by the Korean Ministry of Health and Welfare, and there are 15 types of disabilities. For exercise records, we calculated the cost in 2020 for people with exercise records in 2018 so that they can have at least 1 year of exercise records, as health checkups are available from January to December of the year. Physical activity attainment is defined as 150 min or more of physical activity per week based on World Health Organization guidelines.ResultsCalculating the economic burden of disease by physical activity for persons with and without disabilities, both experienced a difference in hospitalization costs owing to fewer hospital days with physical activity achievement, with the difference being greater for persons with disabilities. Regarding disability type, achievers showed a 41.1% difference in total costs for mental class disorders, 16.7% for external bodily function disorders, and 11.1% for internal organ disorders.DiscussionPhysical inactivity has a significant impact on the economic burden of persons with disabilities compared to persons without disabilities, with 11.8% fewer persons without disabilities who were physically active for 150 min or more per week compared to 21.4% fewer persons with disabilities. In terms of healthcare spending, exercise can help keep persons with disabilities healthy. Therefore, there is a need for exercise programs tailored to persons with disabilities to increase physical activity in line with World Health Organization recommendations to reduce health inequalities between persons with and without disabilities.
Exposure to ambient air pollutions and its association with adverse birth outcomes: a systematic review and meta-analysis of epidemiological studies
Authors: Desye Belay , Berihun Gete , Geto Abebe Kassa , Berhanu Leykun , Daba Chala 
Published: November 2024

IntroductionAir pollution is a significant global public health concern. However, there is a lack of updated and comprehensive evidence regarding the association between exposure to ambient air pollution and adverse birth outcomes (preterm birth, low birth weight, and stillbirth). Furthermore, the existing evidence is highly inconsistent. Therefore, this study aims to estimate the overall association between ambient air pollution and adverse birth outcomes.MethodsIn this study, initially a total of 79,356 articles were identified. Finally, a total of 49 articles were included. We conducted compressive literature searches using various databases, including PubMed, Scientific Direct, HINARI, and Google Scholar. Data extraction was performed using Microsoft Excel, and the data were exported to STATA 17 software for analysis. We used the Joanna Briggs Institute’s quality appraisal tool to ensure the quality of the included studies. A random effects model was employed to estimate the pooled prevalence. Publication bias was assessed using funnel plots and Egger’s regression test.ResultsIn this study, the pooled prevalence of at least one adverse birth outcome was 7.69% (95% CI: 6.70–8.69), with high heterogeneity (I2 = 100%, p-value &lt; 0.001). In this meta-analysis, high pooled prevalence was found in preterm birth (6.36%), followed by low birth weights (5.07%) and stillbirth (0.61%). Exposure to PM2.5 (≤10 μg/m3) throughout the entire pregnancy, PM2.5 (≤10 μg/m3) in the first trimester, PM10 (&gt;10 μg/m3) during the entire pregnancy, and O3 (≤10 μg/m3) during the entire pregnancy increased the risk of preterm birth by 4% (OR = 1.04, 95% CI: 1.03–1.05), 5% (OR = 1.05, 95% CI: 1.01–1.09), 49% (OR = 1.49, 95% CI: 1.41–1.56), and 5% (OR = 1.05, 95% CI: 1.04–1.07), respectively. For low birth weight, exposure to PM2.5 (≤10 μg/m3) and PM2.5 (&gt;10 μg/m3) throughout the entire pregnancy was associated with an increased risk of 13% (OR = 1.13, 95% CI: 1.05–1.21) and 28% (OR = 1.28, 95% CI: 1.23–1.33), respectively.ConclusionThis study highlighted a significant association between ambient air pollution and adverse birth outcomes. Therefore, it is crucial to implement a compressive public health intervention.Systematic review registrationThe review protocol was registered with the record ID of CRD42024578630.
Analyzing the adoption of nutrition-sensitive carp-SIS polyculture technology: evidence from a case study in Bangladesh
Authors: Hossain Nusrat Zaitun , Grote Ulrike , Dubey Sourabh Kumar 
Published: November 2024

IntroductionSmall indigenous species (SIS) of fish are rich in micronutrients that are essential to combat the existing malnutrition in Bangladesh. However, their availability is constantly decreasing due to gradual environmental degradation making their availability irregular and hence expensive in the market. Integrating SIS with carps in homestead ponds is being promoted as a form of nutrition-sensitive aquaculture to enhance both production and consumption of these nutrient-rich species. Various improved pond management techniques (IPMTs) are suggested to boost the nutrition-sensitive carp-SIS polyculture.ObjectivesThis study examines the trends and factors influencing the adoption of IPMTs using the sustainable livelihood framework (SLF) approach. We address the following three key questions: (i) What are the trends in production and consumption of fish, in particular SIS from homestead ponds? (ii) Have farm households adopted or disadopted IPMTs over time? (iii) What are the determinants of adoption and disadoption of IPMTs?MethodsBased on primary data of 234 households from Barishal district from 2014 and 2022, we perform significance tests to compare project and non-project households in 2014 versus 2022 and apply fixed effects Poisson regression and fixed effects negative binomial regression models to identify household decisions to adopt the IPMTs.Results and conclusionQuantitative survey results indicate a significant increase in the production and consumption of SIS over time. In 2014, the project households exhibited adoption rates of 60% or more for various IPMTs such as stocking of fast-growing species, pre-stocking liming, pond dike construction, fertilizer application, stocking of high quality fish seeds, aquatic weed control, and turbidity management. However, by 2022, some of these IPMTs were disadopted by project households and only a few techniques, including providing sunlight exposure to ponds, pre-stocking liming, and supplementary feeding, were more widely used. Additionally, many non-project households also increased their adoption rates of IPMTs significantly. The fixed effects regression model shows that adoption is positively influenced by the number of household members participating in aquaculture, size of the pond, sole ownership of the pond and the number of years household has been involved in aquaculture. Follow-up activities such as monitoring and training of the project beneficiaries and government support are recommended to support long-term adoption of the IPMTs.
Genetic and biological insights into Hydatigera taeniaeformis in invasive black rats from southern Chile
Authors: Alvarez Rojas Cristian A. , Bonacic Cristian , Salgado Rodrigo , Peters Lucia , Fredes Diego , Rubio André V. , Muñoz-Leal Sebastián , Oyarzún-Ruiz Pablo , Aguirre A. Alonso 
Published: November 2024

IntroductionThis study investigates the genetic variability of Hydatigera taeniaeformis in black rats (Rattus rattus), a common tapeworm that infects cats and rodents worldwide. Despite its widespread presence and zoonotic potential, little is known about the genetic diversity of this parasite in the Americas.MethodsWe conducted DNA barcoding analysis using mitochondrial cox1 gene sequences using samples collected from 171 invasive wild black rats, captured in the temperate rainforest of Southern Chile. We also included two adult parasites isolated from road killed Kodkods (Leopardus guigna), a small felid species native to the Americas.ResultsOur findings revealed only two haplotypes, suggesting low genetic variability in a parasite that arrived in the Americas with the Spanish colonization.DiscussionThese haplotypes are more closely related to parasite populations from Peru, Africa, Australia, and Europe, suggesting an origin linked to the Spanish colonization, possibly from North Africa via the Canary Islands. The study also analyzed infection rates, parasite size, and their correlation with host body size, age, and weight, revealing significant patterns. These results provide new insights into the biogeography and genetic diversity of H. taeniaeformis in a new geographical area, enhancing our understanding of its evolutionary history.