bluuebunny/crossref_metadata_2025_split huggingface dataset:
AI on animals: AI-assisted animal-borne logger never misses the moments that biologists want
Authors: Korpela Joseph M. , Suzuki Hirokazu , Matsumoto Sakiko , Mizutani Yuichi , Samejima Masaki , Maekawa Takuya , Nakai Junichi , Yoda Ken 
Published: May 2019

ABSTRACTAnimal-borne data loggers, i.e., biologgers, allow researchers to record a variety of sensor data from animals in their natural environments (Hussey et al. 2015; Kays et al. 2015). This data allows biologists to observe many aspects of the animals’ lives, including their behavior, physiology, social interactions, and external environment. However, the need to limit the size of these devices to a small fraction of the animal’s size imposes strict limits on the devices’ hardware and battery capacities (Kays et al. 2015). Here we show how AI can be leveraged on board these devices to intelligently control their activation of costly sensors, e.g., video cameras, allowing them to make the most of their limited resources during long deployment periods. Our method goes beyond previous works that have proposed controlling such costly sensors using simple threshold-based triggers, e.g., depth-based (Watanuki et al. 2007; Volpov et al. 2015) and acceleration-based (Nishiumi et al. 2018; Brown et al. 2012) triggers. Using AI-assisted biologgers, biologists can focus their data collection on specific complex target behaviors such as foraging activities, allowing them to automatically record video that captures only the moments they want to see. By doing so, the biologger can reserve its battery power for recording only those target activities. We anticipate our work will provide motivation for more widespread adoption of AI techniques on biologgers, both for intelligent sensor control and intelligent onboard data processing. Such techniques can not only be used to control what is collected by such devices, but also what is transmitted off the devices, such as is done by satellite relay tags (Cox et al. 2018).
A genetically validated approach to detect inorganic polyphosphates in plants
Authors: Zhu Jinsheng , Loubéry Sylvain , Broger Larissa , Lorenzo-Orts Laura , Utz-Pugin Anne , Young-Tae Chang , Hothorn Michael 
Published: May 2019

AbstractInorganic polyphosphates (polyPs) are linear polymers of orthophosphate units linked by phosphoanhydride bonds. PolyPs represent important stores of phosphate and energy, and are abundantly found in many pro- and eukaryotic organisms. In plants, the existence of polyPs has been established using microscopy and biochemical extraction methods that are now known to produce artifacts. Here we use a polyP-specific dye and a polyP binding domain to detect polyPs in plant and algal cells. To develop the staining protocol, we induced polyP granules inNicotiana benthamianaand Arabiopsis cells by heterologous expression ofE. colipolyphosphate kinase 1 (PPK1). Over-expression of PPK1 but not of a catalytically impaired version of the enzyme lead to severe growth phenotypes, suggesting that ATP-dependent synthesis and accumulation of polyPs in the plant cytosol is toxic. We next crossed stable PPK1 expressing Arabidopsis lines with plants expressing the polyP-binding domain ofE. coliexopolyphosphatase (PPX1c), which co-localized with PPK1-generated polyP granules. These granules were stained by the polyP-specific dye JC-D7 and appeared as electron dense structures in transmission electron microscopy (TEM) sections. Using the polyP staining protocol derived from these experiments, we screened for polyP stores in different organs and tissues of both mono- and dicotyledonous plants. While we could not detect polyP granules in higher plants, we could visualize the polyP-rich acidocalicisomes in the green algaeChlamydomonas reinhardtii.Together, our experiments suggest that higher plants may not contain large polyPs stores.Significance StatementA chemical dye and an inorganic polyphosphate binding domain are shown to specifically label inorganic polyphosphate granules in transgenic Arabidopsis lines and Chlamydomonas acidocalcisomes. Using these tools, we show that in contrast to many prokaryotic and eukaryotic organisms, higher plants do not seem to contain large inorganic polyphosphate stores.
Amplification of genomic regions harbouring genes with dose-limiting effects provides selection pressure for acquiring aberrant epigenetic silencing in ovarian cancer
Authors: Bonito Nair , Eringyte Ieva , Masrour Nahal , Wilhelm-Benartzi Charlotte , Brown Robert , Curry Edward 
Published: May 2019

ABSTRACTHigh-grade serous ovarian cancer (HGSOC) feature widespread genomic rearrangements that alter the copy number of genes. Genes for which elevated expression is detrimental to growth may be passengers in rearrangements that increase the copy number of strongly selective driver genes. There would then be selection pressure for compensatory epigenetic silencing of such passenger genes, such as through promoter DNA methylation.We have used two independent cohorts of primary HGSOC tumour samples to provide evidence of consistent dosage-compensating promoter methylation (DCPM) genome-wide. Mapping CpG methylation to genomic copy number, we show that bias due to variable tumour cellularity of tissue samples results in false positive associations between methylation and copy number. Adjusting for this bias, we found that approximately 5-10% of all measured promoter CpGs with copy number gain showed a statistically significant increase in methylation. DCPM nullifies the association between increased copy number and increased gene expression. We confirmed a functional basis for selective pressure against over-expressing two candidate passenger genes in the 3q12 locus, as overexpressing either gene reduced spheroid formation efficiency in two HGSOC cell lines. DCPM represents a novel class of specific functional interactions between genetic and epigenetic landscapes of cancers.
Genetic variation regulates the activation and specificity of Restriction-Modification systems in Neisseria gonorrhoeae
Authors: Sánchez-Busó Leonor , Golparian Daniel , Parkhill Julian , Unemo Magnus , Harris Simon R. 
Published: May 2019

ABSTRACTRestriction-Modification systems (RMS) are one of the main mechanisms of defence against foreign DNA invasion and can have an important role in the regulation of gene expression. The obligate human pathogen Neisseria gonorrhoeae carries one of the highest loads of RMS in its genome; between 13 to 15 of the three main types. Previous work has described their organization in the reference genome FA1090 and has experimentally inferred the associated methylated motifs. Here, we studied the structure of RMS and target methylated motifs in 25 gonococcal strains sequenced with Single Molecule Real-Time (SMRT) technology, which provides data on DNA modification. The results showed a variable picture of active RMS in different strains, with phase variation switching the activity of Type III RMS, and both the activity and specificity of a Type I RMS. Interestingly, the Dam methylase was found in place of the NgoAXI endonuclease in two of the strains, despite being previously thought to be absent in the gonococcus. We also identified the real methylation target of NgoAX as 5’-GCAGA-3’, different from that previously described. Results from this work give further insights into the diversity and dynamics of RMS and methylation patterns in N. gonorrhoeae.
Transactional sex among men who have sex with men participating in the CohMSM prospective cohort study in West Africa
Authors: Kounta Cheick Haïballa , Sagaon-Teyssier Luis , Coulaud Pierre-Julien , Mora Marion , Maradan Gwenaelle , Bourrelly Michel , keita Abdoul Aziz , Yoro Stéphane-Alain Babo , Anoma Camille , Coulibaly Christian , Tiero Dah Elias Ter , Agbomadji Selom , Mensah Ephrem , Bernier Adeline , Couderc Clotilde , Keita Bintou Dembélé , Laurent Christian , Spire Bruno ,   the CohMSM Study Group
Published: May 2019

AbstractMen who have sex with men (MSM) are at much greater risk of HIV infection in Africa. Little is known about their involvement in transactional sex (TS). We aimed to characterize MSM reporting TS (MSM-TS) and to identify factors associated with their sexual practices using data from the prospective cohort study CohMSM conducted in Burkina Faso, Côte d’Ivoire, Mali and Togo. Our study focused on HIV-negative MSM, recruited between 06/2015 and 01/2018 by a team of trained peer educators. Scheduled study visits at 6, 12 and 18 months included medical examinations, HIV screening, risk-reduction counselling and face-to-face interviews to collect information on their sociodemographic characteristics, sexual behaviours, and HIV risk-reduction strategies. Three stigmatization sub-scores were constructed. The generalized estimating equation method was used for data analysis. Of the 630 HIV-negative participants recruited at baseline, 463, 410 and 244 had a follow-up visit at 6- and 12- and 18-months, respectively. Over a total of 1747 visits, 478 TS encounters were reported by 289 MSM-TS (45.9%). Of the latter, 91 participants reported systematic TS (31.5%), 55 (19.0 %) stopped reporting TS after baseline, and 53 (18.3%) reported TS after baseline. Ninety participants (31.1 %) reported occasional TS. After adjusting for country of study and follow-up visits, the following factors, reported for the previous 6 months, were associated with a greater likelihood of TS: younger age, an educational level <high-school diploma, satisfaction with current sex life, group sex with men, multiple male sexual partners, condomless anal sex, receptive or versatile anal sex with male sexual partners, giving benefits in exchange for sex with a man, alcohol consumption and drug use during sex, and experiencing stigmatization. The majority of MSM in this study who received benefits in exchange for sex had high-risk HIV infection exposure practices and were characterized by socioeconomic difficulties.
Network governance and the contemporary opium problem in northern Thailand
Authors: Jongruck Patamawadee 
Published: May 2019

PurposeThe purpose of this paper is to explore the current approach that the Thai Government has employed to manage “wicked problems,” using the case of opium in northern Thailand, and analyzing how the network governance approach can contribute to sustainable upland community development.Design/methodology/approachA case study was employed for this research. The data collection was based upon a qualitative research approach, namely in-depth interviews, participatory observation and document research, were all conducted.FindingsThe study found that the strategy of the Thai Government to manage the opium problem in the upland periphery has changed over time. The recent approach could be illustrated as a form of network governance albeit solely within the governmental realm, which was found to be different from the network governance stratagem currently defined in the dominant literature. This paper suggests that in order to achieve a sustainable solution for upland community development, the mode of network governance should shift toward self-governing networks. In other words, non-state stakeholders should be actively engaged in the network and empowered to manage their problems for sustainable upland community development.Originality/valueThis paper contributes to the current corpus of network governance literature by introducing an empirical case study from Thailand. In terms of policy implication, this paper provides policy suggestions for governments, especially in Asia, who are actively seeking to resolve “wicked problems” and achieving sustainable community development.
The effect of social and economic transitions on the meaning of work
Authors: Sharabi Moshe , Polin Brian , Yanay-Ventura Galit 
Published: May 2019

PurposeThe purpose of this paper is to examine the impact of social and economic changes, particularly the transition from a collectivistic to an individualistic society, on the meaning of work (MOW) in Israel.Design/methodology/approachA questionnaire probing the MOW domains (economic orientation, intrinsic orientation, interpersonal relations, entitlement and obligation norms and job satisfaction) was conducted on a representative sample of the Israeli labor force in 1981 (n=973) and 2006 (n=898).FindingsA comparison between the results of the 1981 and 2006 surveys shows a significant increase in economic and intrinsic orientation, and a significant decrease in interpersonal relations orientation, job satisfaction and obligation and entitlement norms. Work centrality remained stable. The demographic factors, especially education, have some impact on the MOW domains.Practical implicationsThe transformation of work values reflects the changes in employees’ expectations and needs. Therefore, organizations are compelled to find new material and non-material reward systems and methods to maintain employee commitment, motivation and satisfaction. Various implications for organizations and management are discussed.Originality/valueThis cross-sectional study explores how the combination of economic and social changes is reflected in employees’ job satisfaction and other work values. Furthermore, the study examines the impact of the main demographic factors on the MOW and offers suggestions to gain employees loyalty and commitment based on all the findings.
Adolescents’ learning and experiences of solving the need for dietary supplementation through socioscientific issue (SSI) method
Authors: Kärkkäinen Sirpa , Hartikainen-Ahia Anu , Elorinne Anna-Liisa , Hokkanen Johanna , Hämeen-Anttila Katri 
Published: May 2019

PurposeThe purpose of this paper is to assess the socioscientific issue (SSI) method as a way of improving seventh graders’ nutrition know-how on dietary supplements (DS), and to describe how students experience learning nutritional issues through the SSI method.Design/methodology/approachParticipants were seventh graders from three different classes (n=43). Health education intervention lasted 7 h and it embodied the three-staged model: the scenario, the inquiry and the decision-making stages. The study was qualitative in nature. The data were collected through a questionnaire before and after the intervention. The data were analyzed using summative content analysis.FindingsIntervention tended to deepen students’ know-how on vitamins and rational use of DS. Students’ experiences of learning nutritional issues through the SSI method were generally positive. The scenario stage was considered current and authentic. The inquiry stage and decision-making stage helped in understanding the societal connections between the study task and the rational use of DS.Practical implicationsSSI method enhances students’ knowledge about the rational use of DS. E-learning environment gave students possibilities to work collaboratively. Especially, possibilities to discuss with peers and share own experiences supported learning.Originality/valueTo solve the need of dietary supplements (DS) is a complex nutritional issue for society and for individuals. Since consumption of special diets has increased among adolescents, the rational consumption of DS is relevant and interesting learning topic at secondary school.
A survey on VV&A of large-scale simulations
Authors: Wang Yanan , Li Jianqiang , Hongbo Sun , Li Yuan , Akhtar Faheem , Imran Azhar 
Published: May 2019

PurposeSimulation is a well-known technique for using computers to imitate or simulate the operations of various kinds of real-world facilities or processes. The facility or process of interest is usually called a system, and to study it scientifically, we often have to make a set of assumptions about how it works. These assumptions, which usually take the form of mathematical or logical relationships, constitute a model that is used to gain some understanding of how the corresponding system behaves, and the quality of these understandings essentially depends on the credibility of given assumptions or models, known as VV&A (verification, validation and accreditation). The main purpose of this paper is to present an in-depth theoretical review and analysis for the application of VV&A in large-scale simulations.Design/methodology/approachAfter summarizing the VV&A of related research studies, the standards, frameworks, techniques, methods and tools have been discussed according to the characteristics of large-scale simulations (such as crowd network simulations).FindingsThe contributions of this paper will be useful for both academics and practitioners for formulating VV&A in large-scale simulations (such as crowd network simulations).Originality/valueThis paper will help researchers to provide support of a recommendation for formulating VV&A in large-scale simulations (such as crowd network simulations).
Hybrid impedance control of a knee joint orthosis
Authors: Alouane Mohamed Amine , Rifai Hala , Kim Kwangtaek , Amirat Yacine , Mohammed Samer 
Published: May 2019

Purpose
This paper aims to deal with the design of new hybrid approach for the assistance of the flexion extension movement of the knee joint.

Design/methodology/approach
The control approach combines the use of a knee joint orthosis along with functional electrical stimulation (FES) within an assist-as-needed paradigm. An active impedance controller is used to assist the generation of muscular stimulation patterns during the extension sub-phase of the knee joint movement. The generated FES patterns are appropriately tailored to achieve flexion/extension movement of the knee joint, which allows providing the required assistance by the subject through muscular stimulation. The generated torque through stimulation is tracked by a non-linear disturbance observer and fed to the impedance controller to generate the desired trajectory that will be tracked using a standard proportional derivative controller.

Findings
The approach was tested in experiments with two healthy subjects. Results show satisfactory performances in terms of estimating the knee joint torque, as well as in terms of cooperation between the FES and the orthosis actuator during the execution of the knee joint flexion/extension movements.

Originality/value
The authors designed a new hybrid approach for the assistance of the flexion extension movement of the knee joint, which has not been studied yet. The control approach combines the use of a knee joint orthosis along with FES within an assist-as-needed paradigm.
Displacing Informality: Rights and Legitimacy in Belo Horizonte, Brazil
Authors: Nogueira Mara 
Published: May 2019

AbstractThis article compares two cases of displacement suffered by informal workers and informal residents in the Brazilian city of Belo Horizonte, both connected to the hosting of the 2014 FIFA World Cup. It asks the following question: considering that the right to work and the right to housing are both enshrined in the Brazilian Constitution, why do claims upon space based on those constitutional rights have different degrees of legitimacy? Two cases are analysed in detail. The first one concerns a group of informal workers displaced from their workspace for the modernization of the local stadium. The second one tells the story of an informal settlement where 90 families were displaced due to the construction of a flyover designed to improve access to the football stadium. This article engages with current postcolonial debates around urban informality, tackling two points that have been absent from these discussions. First, it compares two ways of informally occupying urban space—for work and for housing—revealing the distinct degrees of legitimacy embedded in such practices due to pre‐existing institutional arrangements. Second, it emphasizes the connection between work and home through the life strategies and place‐making practices of the urban poor.
Geochemical and Geological Characterization of Marine‐Continental Transitional Shale: A Case Study in the Ordos Basin, NW China
Authors: WEI Zhifu , WANG Gen , WANG Yongli , MA Xueyun , ZHANG Ting , HE Wei , YU Xiaoli 
Published: May 2019

AbstractThe organic‐rich shale of the Shanxi and Taiyuan Formation of the Lower Permian deposited in a marine‐continental transitional environment are well developed in the Ordos Basin, NW China, which is considered to contain a large amount of shale hydrocarbon resources. This study takes the Lower Permian Shanxi and Taiyuan shale collected from well SL# in the Ordos Basin, NW China as an example to characterize the transitional shale reservoir. Based on organic geochemistry data, X‐ray diffraction (XRD) analysis, field‐emission scanning electron microscopy (FE‐SEM) observations, the desorbed gas contents of this transitional shale were systematically studied and the shale gas potential was investigated. The results indicate that the Lower Permian Shanxi and Taiyuan shale has a relatively high total organic carbon (TOC) (average TOC of 4.9%) and contains type III kerogen with a high mature to over mature status. XRD analyses show that an important characteristic of the shale is that clay and brittle minerals of detrital origin comprise the major mineral composition of the marine‐continental transitional shale samples, while the percentages of carbonate minerals, pyrite and siderite are relatively small. FE‐SEM observations reveal that the mineral matrix pores are the most abundant in the Lower Permian shale samples, while organic matter (OM) pores are rarely developed. Experimental analysis suggests that the mineral compositions mainly govern the macropore development in the marine‐continental transitional shale, and mineral matrix pores and microfractures are considered to provide space for gas storage and migration. In addition, the desorption experiments demonstrated that the marine‐continental transitional shale in the Ordos Basin has a significantly potential for shale gas exploration, ranging from 0.53 to 2.86 m3/t with an average value of 1.25m3/t, which is in close proximity to those of terrestrial shale (1.29 m3/t) and marine shale (1.28 m3/t). In summary, these results demonstrated that the Lower Permian marine‐continental transitional shale in the Ordos Basin has a significantly potential for shale gas exploration.
Does Fair Value Accounting Contribute to Systemic Risk in the Banking Industry?
Authors: Khan Urooj 
Published: May 2019

ABSTRACTI investigate whether fair value accounting can contribute to the banking industry's systemic risk. I focus on the adoption of Statement of Financial Accounting Standard No. 115 (SFAS No. 115), which required available‐for‐sale (AFS) securities to be recognized at fair value with unrealized gains and losses included in equity through accumulated other comprehensive income. SFAS No. 115 increased banks' regulatory risk because, at the time, calculation of regulatory capital closely conformed with GAAP equity. I find that systemic risk increased following the adoption of SFAS No. 115. Furthermore, following a subsequent regulatory amendment—which excluded unrealized gains and losses on AFS securities from regulatory capital but did not change their GAAP treatment—systemic risk decreased. Taken together, the evidence suggests that fair value accounting has the potential to increase systemic risk through the explicit inclusion of volatile fair value estimates in regulatory bank capital adequacy assessments. I do not, however, find evidence of fair value accounting impacting systemic risk in its information role; that is, by providing information to a bank's external stakeholders about its financial position and performance. I also show that higher fair value volatility of investment securities, lower bank capital, and larger AFS security holdings increase banks' marginal contribution to systemic risk. My findings should interest regulators and policymakers, as recent regulatory changes in light of Basel III recommendations require unrealized gains and losses on AFS securities to be included in regulatory capital for advanced approaches banks.