bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimentally Induced Biliary Atresia by Means of Rotavirus‐Infection Is Directly Linked to Severe Damage of the Microvasculature in the Extrahepatic Bile Duct
Authors: Oetzmann von Sochaczewski Christina , Pintelon Isabel , Brouns Inge , Thys Sofie , Deigendesch Nikolaus , Kübler Joachim F. , Timmermans Jean‐Pierre , Petersen Claus 
Published: October 2018

ABSTRACTVascular damage has been reported to contribute to atresia formation in several diseases including biliary atresia. This study focused on the extrahepatic biliary plexus in experimental biliary atresia. Newborn BALB/cAnNCrl‐pups were infected with rhesus rotavirus within 24 hr after birth to induce experimental biliary atresia. The extrahepatic biliary plexus was examined by confocal microscopy on whole‐mount preparations, scored by three independent researchers, and further evaluated at the subcellular level with transmission electron microscopy. Imaging results revealed a progressive destruction of the extrahepatic biliary vascular plexus in the course of experimental biliary atresia induced by rotavirus infection. Endothelial cell damage was already visible as cell swelling and necrosis in the first days after infection and a damaged microcirculation that rapidly deteriorated with progression of obliterative cholangiopathy, was observed in the infected mice as early as 72 hr after birth. In experimental biliary atresia, the destruction of the extrahepatic biliary vascular plexus starts already in the first days postinfection and clearly precedes the morphological symptoms of atresia. The deterioration of the vascular bed architecture continues with disease progression. Therefore, we conclude that the (ultra)structural changes in the extrahepatic biliary microvasculature occurring before the visible onset of atresia has a predictive diagnostic value and this impairment in blood supply to the extrahepatic bile duct may be an important contributing factor to the pathogenesis of acquired biliary atresia. Anat Rec, 2018. © 2018 Wiley Periodicals, Inc. Anat Rec, 302:818–824, 2019. © 2018 Wiley Periodicals, Inc.
Oxidation of Guanosine Monophosphate with O2 via a Ru‐peroxo Complex in Water
Authors: Takenaka Makoto , Kikkawa Mitsuhiro , Matsumoto Takahiro , Yatabe Takeshi , Ando Tatsuya , Yoon Ki‐Seok , Ogo Seiji 
Published: October 2018

AbstractOxidative damage of DNA by reactive oxygen species (ROS) is responsible for aging and cancer. Although many studies of DNA damage by ROS have been conducted, there have been no reports of the oxidation of RNA components, such as guanosine monophosphate, by metal‐based species in water. Here, we report the first case of oxidation of guanosine monophosphate to 8‐oxoguanosine monophosphate by a metal‐based oxygen bound species, derived from O2 and in water.
Portable fluorescence‐based microRNA detection system based on isothermal signal amplification technology
Authors: Zhang Zhanying , Wang Yanfei , Li Yanlei , Yu Dongsheng , Chen Haiyan , Cai Yi , Fang Weikai , Yang Zhe , Ji Yufeng , Guan Yifu , Chu Yannan , Xu Chidong 
Published: October 2018

AbstractMicroRNAs (miRNAs) diagnostics can be useful for diagnosing or confirming miRNA abundance and are used in screening tests and to assess changes in miRNAs in vivo. At present, the use of traditional nucleic acid amplification assays to detect miRNAs has been limited in laboratory environment because of the time, equipment, and technical expertise required to perform these assays. A specialized, rapid affordable miRNA detection system is necessary when there are limited resources or point‐of‐care testing needs. We designed a portable and affordable fluorescence‐based miRNA detection system based on isothermal signal amplification technology, using SYBR Green II as a fluorescent dye. To reduce costs, we chose LED as a light source and designed the corresponding optical path for LED. The portable detection system shows results consistent with those by real‐time PCR, and can be used to detect miR‐183 with a limit of detection of approximately 2 fmol. We used the system to detect miR‐183 in tissues and blood from patients with hepatocellular carcinoma (HCC). The results from the portable detection device were compared with those from clinical trials and indicated that the miR‐183 fluorescence signal could successfully identify HCC and provide information related to cancer progression.
Minderung von Erschütterungs‐ und Körperschalleinträgen von Seilbahnen in ein Gebäude
Authors: Gömmel Andreas 
Published: October 2018

AbstractFür die Schwingungs‐ und Körperschallentkopplung von insgesamt zwei Stützen, zwei Umlenktürmen und einer Antriebsstation einer ringförmigen Seilbahn auf einem neu errichteten Gebäude in Asien wurde ein Konzept zur elastischen Lagerung mittels bewehrter Gummielemente entwickelt. Ziel war es, sowohl die Schwingungsimmissionen als auch den abgestrahlten Luftschall aus Körperschallübertragung zu begrenzen. Die Auslegung erfolgte mithilfe dreidimensionaler Finite‐Elemente‐Modelle. Die Schwierigkeit bestand vor allem darin, die Anregungskräfte wirklichkeitsnah zu erfassen sowie die Eingangsimpedanz des Gebäudes realistisch abzuschätzen. In einer aufwändigen Abnahmemessung vor Ort konnten während der Inbetriebnahme der Seilbahn die Wirksamkeit der Konstruktionen und die Einhaltung der vertraglich vereinbarten Werte nachgewiesen werden.
Economic comparative advantage of willow biomass in the Northeast USA
Authors: Langholtz Matthew , Eaton Laurence , Davis Maggie , Shedden Magen , Brandt Craig , Volk Tim , Richard Tom 
Published: October 2018

AbstractWe evaluate the potential economic availability of willow (Salix spp.) short‐rotation woody crops in the Northeast USA. Based on results from a 20‐year agronomic simulation, the Northeast USA could potentially provide between 2.1 and 9.7 million dry Mg year−1, depending on farmgate price and competition with other energy crops. In a diversified biomass energy‐crop scenario, willow outperforms other lignocellulosic energy crops in planted area and production at 91% of supply and 83% of planted area in a low‐price scenario in the Northeast USA. In a high‐price scenario, willow also outperforms competing energy crops at 47% of production and 51% of planted area. In contrast with the rest of the USA, willow comprises the greatest portion of energy crop potential in the Northeast. These results suggest that willow has an agronomic comparative advantage in this region as compared to herbaceous energy crops, with great potential to increase production given adequate market prices. © 2018 The Authors. Biofuels, Bioproducts, and Biorefining published by Society of Chemical Industry and John Wiley & Sons, Ltd.
The effects of fluorescent labels on Aβ42 aggregation detected by fluorescence correlation spectroscopy
Authors: Zheng Yanpeng , Xu Lingwan , Yang Jingfa , Peng Xianglei , Wang He , Yu Na , Hua Ying , Zhao Jiang , He Jinsheng , Hong Tao 
Published: October 2018

AbstractFluorescence‐based methods are promising for measuring amyloid beta (Aβ) oligomers, given their capacity to analyse a sample at the single‐molecule level. As the attachment of fluorescent labels may influence the biochemical properties of the Aβ oligomers, the effects of fluorescent labels on Aβ oligomers must be evaluated. In this paper, we compared the impacts of five different fluorescent dyes on the aggregation of Aβ42 oligomers using fluorescence correlation spectroscopy (FCS). We found that fluorescent labels of BODIPY® FL‐C5 (BP), N‐hydroxysuccinimide rhodamine B ester (RB) and rhodamine B isothiocyanate (RITC) increased the propensity of labelled Aβ42 oligomers to aggregate, whereas 6‐(fluorescein‐5‐carboxamido) hexanoic acid succinimidyl ester (5‐SFX) and fluorescein 5(6)‐isothiocyanate (5(6)‐FITC) decreased the propensity of labelled Aβ42 oligomers to aggregate. This difference originated from the different electric charges and hydrophobicity of the fluorescent dyes. These results provide valuable information for establishing different aggregation models for Aβ42 oligomers in vitro using FCS.
Meta-analysis of sentinel lymph node biopsy after neoadjuvant chemotherapy in patients with initial biopsy-proven node-positive breast cancer
Authors: Tee S R , Devane L A , Evoy D , Rothwell J , Geraghty J , Prichard R S , McDermott E W 
Published: October 2018

Abstract

Background
Neoadjuvant chemotherapy for breast cancer has the potential to achieve a pathological complete response in up to 40 per cent of patients, converting disease that was initially node-positive to node-negative. This has raised the question of whether sentinel lymph node biopsy could be an alternative to axillary lymph node dissection in these patients. The aim was to undertake a systematic review and meta-analysis of the accuracy and reliability of sentinel lymph node biopsy after neoadjuvant chemotherapy in patients with initial biopsy-proven node-positive breast cancer.

Methods
A literature search was conducted using PubMed, Ovid MEDLINE, Embase and Web of Science databases up to 30 April 2017. Inclusion criteria for studies were pathological confirmation of initial node-positive disease, and sentinel lymph node biopsy performed after neoadjuvant chemotherapy followed by axillary lymph node dissection.

Results
A total of 13 studies met the inclusion criteria and were included in the analysis (1921 patients in total). The pooled estimate of identification rate was 90 (95 per cent c.i. 87 to 93) per cent and the false-negative rate was 14 (11 to 17) per cent. In subgroup analysis, the false-negative rate with use of dual mapping was 11 (6 to 15) per cent, compared with 19 (11 to 27) per cent with single mapping. The false-negative rate was 20 (13 to 27) per cent when one node was removed, 12 (5 to 19) per cent with two nodes removed and 4 (0 to 9) per cent with removal of three or more nodes.

Conclusion
Sentinel lymph node biopsy after neoadjuvant chemotherapy in patients with biopsy-proven node-positive breast cancer is accurate and reliable, but requires careful patient selection and optimal surgical techniques.
Systematic review of treatment intensification using novel agents for chemoradiotherapy in rectal cancer
Authors: Clifford R , Govindarajah N , Parsons J L , Gollins S , West N P , Vimalachandran D 
Published: October 2018

Abstract

Background
With the well established shift to neoadjuvant treatment for locally advanced rectal cancer, there is increasing focus on the use of radiosensitizers to improve the efficacy and tolerability of radiotherapy. There currently exist few randomized data exploring novel radiosensitizers to improve response and it is unclear what the clinical endpoints of such trials should be.

Methods
A qualitative systematic review was performed according to the PRISMA guidelines using preset search criteria across the PubMed, Cochrane and Scopus databases from 1990 to 2017. Additional results were generated from the reference lists of included papers.

Results
A total of 123 papers were identified, of which 37 were included; a further 60 articles were obtained from additional referencing to give a total of 97 articles. Neoadjuvant radiosensitization for locally advanced rectal cancer using fluoropyrimidine-based chemotherapy remains the standard of treatment. The oral derivative capecitabine has practical advantages over 5-fluorouracil, with equal efficacy, but the addition of a second chemotherapeutic agent has yet to show a consistent significant efficacy benefit in randomized clinical assessment. Preclinical and early-phase trials are progressing with promising novel agents, such as small molecular inhibitors and nanoparticles.

Conclusion
Despite extensive research and promising preclinical studies, a definite further agent in addition to fluoropyrimidines that consistently improves response rate has yet to be found.
Epidemiology and secular trends of malignant lymphoma in Japan: Analysis of 9426 cases according to the World Health Organization classification
Authors: Muto Reiji , Miyoshi Hiroaki , Sato Kensaku , Furuta Takuya , Muta Hiroko , Kawamoto Keisuke , Yanagida Eriko , Yamada Kyohei , Ohshima Koichi 
Published: October 2018

AbstractThis study provides an overview of the epidemiology and secular trends of malignant lymphoma in Japan. Using data from clinics and hospitals throughout Japan, we analyzed 9426 cases of malignant lymphoma diagnosed in 2007‐2014. We show that the proportion of follicular lymphoma and methotrexate‐associated lymphoproliferative disorder increased during this time, as did the onset age for follicular lymphoma and diffuse large B‐cell lymphoma. Significant increases in onset age for follicular lymphoma and diffuse large B‐cell lymphoma were observed in both men and women (all P values <0.0001 except for P = 0.0448 for the latter disease in women). Further studies are required to determine the reasons for the higher proportion of and onset age for these lymphomas. Additionally, we believe that continued observation of these trends is necessary.
Safety and efficacy of self‐apposing Stentys drug‐eluting stent in left main coronary artery PCI: Multicentre LM‐STENTYS registry
Authors: Wańha Wojciech , Mielczarek Maksymilian , Smolka Grzegorz , Roleder Tomasz , Jaguszewski Miłosz , Ciećwierz Dariusz , Tomasiewicz Brunon , Kubler Piotr , Gorol Jarosław , Chmielecki Michał , Bartuś Stanisław , Navarese Eliano Pio , Kasprzak Michał , Sukiennik Adam , Kubica Jacek , Lekston Andrzej , Hawranek Michał , Reczuch Krzysztof , Gruchała Marcin , Ochała Andrzej , Wojakowski Wojciech 
Published: October 2018

AbstractBackgroundThere is a paucity of data on left main (LM) percutaneous coronary intervention (PCI) therapy with dedicated DES platforms. The LM‐STENTYS is a multicenter registry aimed at evaluating clinical outcome after PCI of LM performed with a self‐apposing Stentys DES implantation.MethodsThe registry consists of 175 consecutive patients treated with Stentys DES implanted to LM. The primary endpoint was the composite of major adverse cardiac and cerebral events (MACCE) defined as cardiac death, myocardial infarction (MI), target lesion revascularization (TLR), and stroke assessed after 1 year. The secondary endpoint was stent thrombosis (ST) at 1 year.ResultsThe median age was 69 years (IQR, 62–78 years). Acute coronary syndrome (ACS) was the presenting diagnosis in 117 (66.9%) patients [74 (63.2%) unstable angina, 31 (26.5%) NSTEMI, 12 (10.3%) STEMI] and stable angina (SA) was present in 58 (33.1%) patients. The median SYNTAX score was 23.0 (IQR, 18.7–32.2) in the SA group and 25.0 (IQR, 20.0–30.7) in the ACS group. During 1‐year follow‐up in the SA group two (3.4%) MACCE occurred, both of them were cardiac deaths. In ACS patients there were 19 (16.2%) MACCE [9 (7.7%) cardiac deaths, 11 (9.4%) MIs, 11(9.4%) TLR, 1(0.9%) stroke]. Altogether, three (1.7%) cases of acute ST were noted, all of them in ACS subset.ConclusionLM PCI using self‐apposing Stentys DES showed favorable clinical outcomes at 1‐year in patients with SA. Events of ST in the ACS group warrant further research.
Impact of residual coronary atherosclerosis on transfemoral transcatheter aortic valve replacement
Authors: Li Jun , Patel Sandeep M. , Nadeem Fahd , Thakker Prashanth , Al‐Kindi Sadeer , Thomas Rahul , Makani Amber , Hornick John M. , Patel Toral , Lipinski Jerry , Ichibori Yasuhiro , Davis Angela , Markowitz Alan H. , Bezerra Hiram G. , Simon Daniel I. , Costa Marco A. , Kalra Ankur , Attizzani Guilherme F. 
Published: October 2018

AbstractObjectivesThis study reports on the clinical effects of complete vs incompletely revascularized coronary artery disease on transcatheter aortic valve replacement (TAVR).BackgroundThere is a high prevalence of active coronary artery disease (CAD) in patients undergoing TAVR but preemptive revascularization remains controversial.MethodsPatients were categorized into three cohorts: complete revascularization (CR), incomplete revascularization of a major epicardial artery (IR Major), and incomplete revascularization of a minor epicardial artery only (IR Minor). When feasible, SYNTAX scoring was performed for exploratory analysis. Analyses were performed using Cox proportional hazard models and Kaplan–Meier method.ResultsA total of 323 patients with active CAD were included. Adjusted outcomes showed that patients with IR Major had increased incidence of acute myocardial infarction (AMI) or revascularization compared with those in the CR cohort (HR 3.72, P = 0.048). No difference was noted in all‐cause mortality or all‐cause readmission rates. Exploratory secondary analysis with residual SYNTAX scores showed a significant interaction between disease burden and AMI/revascularization, as well as all‐cause readmission. All‐cause mortality remained unaffected based on residual SYNTAX scores.ConclusionsThis is a retrospective single‐center study reporting on pre‐TAVR revascularization outcomes in patients with active CAD. In this analysis, we found that patients undergoing TAVR benefited from achieving complete revascularization to abate future incidence of AMI/revascularization. Despite this finding, all‐cause mortality remained unaffected. Future efforts should focus on the role of functional assessment of the coronaries, as well as the long‐term effects of complete revascularization in a larger patient cohort.
Hemodynamic durability of transcatheter aortic valves using the updated Valve Academic Research Consortium‐2 criteria
Authors: Kumar Arnav , Sato Kimi , Banerjee Kinjal , Narayanswami Jyoti , Betancor Jorge , Menon Vivek , Mohananey Divyanshu , Anumandla Anil Kumar , Sawant Abhishek C. , Krishnaswamy Amar , Tuzcu E. Murat , Jaber Wael , Mick Stephanie , Svensson Lars G. , Popović Zoran B. , Blackstone Eugene H , Kapadia Samir R. 
Published: October 2018

AbstractObjectivesWe investigated the hemodynamic durability of the transcatheter aortic valves (TAVs) using the updated Valve Academic Research Consortium‐2 (VARC‐2) criteria.BackgroundThe updated VARC‐2 consensus criteria combine flow‐dependent and flow‐independent echocardiographic parameters for hemodynamic assessment of TAVR. Data on the hemodynamic durability of TAV and clinical risk factors associated with valve hemodynamic deterioration (VHD) are lacking.MethodsAll patients (n = 276) who received TAV between 2006 and 2012 and had ≥2 follow‐up echocardiograms were studied.ResultsDuring a median follow up period of 3.3 (1.8‐4.4) years, 8 patients (3%) developed moderate to severe valve stenosis per the VARC‐2 criteria, while 20 had mild stenosis. In a Cox proportional hazards model analysis, moderate to severe stenosis by VARC‐2 criteria was associated with younger age (P = 0.03, HR 0.94), absence of dual antiplatelet therapy (DAPT) (P = 0.026, HR 0.18), and lower baseline left ventricular ejection fraction (LVEF) (P = 0.006, HR 0.94). Longitudinal analysis using a mixed effect model showed that presence of stenosis by VARC‐2 criteria was associated with an increase in aortic valve mean gradient (P < 0.001, +2.34 mmHg per year). In a subset of 93 patients with analyzable fluoroscopic images, deeper valve implantation was associated with increase in mean gradient (P = 0.004, +0.2 mmHg per year per 1 mm increase in implantation depth).ConclusionDespite good hemodynamic durability of TAV, patients with younger age, lower LVEF and those not on DAPT after undergoing a TAV replacement, are at a higher risk for development of VHD.
Lipase and Laccase Encapsulated on Zeolite Imidazolate Framework: Enzyme Activity and Stability from Voltammetric Measurements
Authors: Naseri Maryam , Pitzalis Federica , Carucci Cristina , Medda Luca , Fotouhi Lida , Magner Edmond , Salis Andrea 
Published: October 2018

AbstractAbstract: Lipase (Pseudomonas fluorescens) and laccase (Trametates versicolor) were encapsulated on two zeolite imidazolate framework, ZIF‐8 and ZIF‐zni, materials using a one‐pot synthesis‐immobilization method in aqueous solution at room temperature. The synthesized immobilized biocatalysts (Lip@ZIF‐8, Lip@ZIF‐zni, Lac@ZIF‐8, and Lac@ZIF‐zni) were characterized by X‐ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The enzymatic activities of the four immobilized biocatalysts were characterized via the electrochemical detection of the substrates, p‐nitrophenyl butyrate and 2,2‐azinobis‐3‐ethylbenzthiazoline‐6‐sulfonic acid. For Lip@ZIF‐8 the specific activity was 91.9 U mg−1 and 123.1 U mg−1 for Lip@ZIF‐zni, while for Lac@ZIF‐8 and Lac@ZIF‐zni, the activity was 51 U mg−1 and 163 U mg−1, respectively, confirming that laccase retains a higher level of activity when immobilized onto ZIF‐zni than on ZIF‐8. Lac@ZIF‐8 was the most stable system on storage (15 days at 5 °C), retaining 94 % of initial activity, while Lip@ZIF‐zni biocatalyst had the optimal level of reusability, retaining 40 % of initial activity after five reaction cycles.
Dual Gold‐Catalyzed Formal Tetradehydro‐Diels–Alder Reactions for the Synthesis of Carbazoles and Indolines
Authors: Wang Hong‐Fa , Wang Shi‐Yue , Qin Tian‐Zhu , Zi Weiwei 
Published: October 2018

AbstractThis work reports a dual gold‐catalyzed tetradehydro‐Diels–Alder reaction for the synthesis of nitrogen‐containing aromatic heterocycles. Under the catalytic system (IPrAuNTf2/DIPEA), indolines and carbazoles as well as other N‐containing aromatic heterocycles were prepared in high yields with good functional group tolerance. Unlike the traditional thermal tetradehydro‐Diels–Alder reactions, diluted reaction concentration and radical prohibitors are not required for this protocol. Experimental data support a mechanism involving gold vinylidene species, which undergoes a 6 π electrocyclization, followed with 1,2‐hydrogen shift.
Activation of Molecular Hydrogen by Arylcarbenes
Authors: Mendez‐Vega Enrique , Maehara Mika , Raut Akshay Hemant , Mieres‐Perez Joel , Tsuge Masashi , Lee Yuan‐Pern , Sander Wolfram 
Published: October 2018

AbstractThe hydrogenation reactions of diphenylcarbene1, fluorenylidene2, and dibenzocycloheptadienylidene3were investigated in solid H2and D2matrices and in H2‐ and D2‐doped argon matrices at cryogenic temperatures. The reactivity of the carbenes towards H2increases in the order1<3<2. Whereas1is stable in solid H2,2and3react fast under the same conditions via quantum chemical tunneling. In D2both1and3are stable, whereas2slowly reacts. The different reactivity of the three carbenes is rationalized in terms of differing carbene stabilization energies.
Silyl Group‐Directed 6‐exo‐dig Iodocyclization of Homopropargylic Carbamates and Amides
Authors: Okitsu Takashi , Nakahigashi Hikaru , Sugihara Ryosuke , Fukuda Ikki , Tsuji Saki , In Yasuko , Wada Akimori 
Published: October 2018

AbstractIodocyclization of silyl group‐substituted homopropargylic carbamates and amides proceeded via 6‐exo‐dig mode to afford 6‐vinylene‐4,5‐dihydro‐1,3‐oxazines in moderate to quantitative yields. This is the first report for silyl group‐solely directed iodocyclization of alkynes utilizing the β‐silyl effect. Under these mild reaction conditions, various functionalities such as secondary alcohol, acetal, urea, and sulfide were tolerated.
Reversible Self‐Assembling of Boryl Radical Anions to Their Diradicals with Tunable Singlet Ground States
Authors: Feng Rui , Zhang Li , Chen Chao , Fang Yong , Zhao Yue , Tan Gengwen , Wang Xinping 
Published: October 2018

AbstractTwo novel boron‐centered diradicals based on dimesitylpyridine borane (1) were synthesized by the self‐assembling of the corresponding radical sodium and potassium salts, respectively. The sodium diradical was obtained by re‐dissolving the crystals of the radical salt 1Na in toluene, while the potassium diradical was directly obtained by the reduction of 1 with potassium in THF. The diradicals could be converted back to their radical anions in THF solution, forming a reversible process. EPR spectroscopy and SQUID measurements, together with theoretical calculations, show that the diradicals have singlet ground states with excited triplet states. Their singlet–triplet energy gaps are tunable with metals.