bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnetized hybrid stars: effects of slow and rapid phase transitions at the quark–hadron interface
Authors: Mariani Mauro , Orsaria Milva G , Ranea-Sandoval Ignacio F , Lugones Germán 
Published: August 2019

ABSTRACT
We study the influence of strong magnetic fields in hybrid stars, composed by hadrons and a pure quark matter core, and analyse their structure and stability as well as some possible evolution channels due to the magnetic field decay. Using an ad hoc parametrization of the magnetic field strength and taking into account Landau-quantization effects in matter, we calculate hybrid magnetized equations of state and some associated quantities, such as particle abundances and matter magnetization, for different sets of parameters and different magnetic field strengths. Moreover, we compute the magnetized stable stellar configurations, the mass versus radius and the gravitational mass versus central energy density relationships, the gravitational mass versus baryon mass diagram, and the tidal deformability. Our results are in agreement with both, the $\sim 2\, \mathrm{M}_\odot$ pulsars and the data obtained from GW170817. In addition, we study the stability of stellar configurations assuming that slow and rapid phase transitions occur at the sharp hadron–quark interface. We find that, unlike in the rapid transition scenario, where ∂M/∂ϵc < 0 is a sufficient condition for instability, in the slow transition scenario there exists a connected extended stable branch beyond the maximum mass star, for which ∂M/∂ϵc < 0. Finally, analysing the gravitational mass versus baryon mass relationship, we have calculated the energy released in transitions between stable stellar configurations. We find that the inclusion of the magnetic field and the existence of new stable branches allows the possibility of new channels of transitions that fulfil the energy requirements to explain gamma-ray bursts.
A magnetic white dwarf with five H α components
Authors: Kilic Mukremin , Rolland B , Bergeron P , Vanderbosch Z , Benni P , Garlitz J 
Published: August 2019

ABSTRACT
G183−35 is an unusual white dwarf that shows an H α line split into five components, instead of the usual three components seen in strongly magnetic white dwarfs. Potential explanations for the unusual set of lines include a double degenerate system containing two magnetic white dwarfs and/or rotational modulation of a complex magnetic field structure. Here, we present time-resolved spectroscopy of G183−35 obtained at the Gemini Observatory. These data reveal two sets of absorption lines that appear and disappear over a period of about 4 h. We also detect low-level (0.2 per cent) variability in optical photometry at the same period. We demonstrate that the spectroscopic and photometric variability can be explained by the presence of spots on the surface of the white dwarf and a change in the average field strength from about 4.6 to 6.2 MG. The observed variability is clearly due to G183−35’s relatively short spin period. However, rotational modulation of a complex magnetic field by itself cannot explain the changes seen in the central H α component. An additional source of variability in the line profiles, most likely due to a chemically inhomogeneous surface composition, is also needed. We propose further observations of similar objects to test this scenario.
Disappearance of Icelandic Walruses Coincided with Norse Settlement
Authors: Keighley Xénia , Pálsson Snæbjörn , Einarsson Bjarni F , Petersen Aevar , Fernández-Coll Meritxell , Jordan Peter , Olsen Morten Tange , Malmquist Hilmar J 
Published: August 2019

AbstractThere is a growing body of evidence demonstrating the impacts of human arrival in new “pristine” environments, including terrestrial habitat alterations and species extinctions. However, the effects of marine resource utilization prior to industrialized whaling, sealing, and fishing have largely remained understudied. The expansion of the Norse across the North Atlantic offers a rare opportunity to study the effects of human arrival and early exploitation of marine resources. Today, there is no local population of walruses on Iceland, however, skeletal remains, place names, and written sources suggest that walruses existed, and were hunted by the Norse during the Settlement and Commonwealth periods (870–1262 AD). This study investigates the timing, geographic distribution, and genetic identity of walruses in Iceland by combining historical information, place names, radiocarbon dating, and genomic analyses. The results support a genetically distinct, local population of walruses that went extinct shortly after Norse settlement. The high value of walrus products such as ivory on international markets likely led to intense hunting pressure, which—potentially exacerbated by a warming climate and volcanism—resulted in the extinction of walrus on Iceland. We show that commercial hunting, economic incentives, and trade networks as early as the Viking Age were of sufficient scale and intensity to result in significant, irreversible ecological impacts on the marine environment. This is to one of the earliest examples of local extinction of a marine species following human arrival, during the very beginning of commercial marine exploitation.
Ancient Mitochondrial Gene Transfer between Fungi and the Orchids
Authors: Sinn Brandon T , Barrett Craig F 
Published: August 2019

AbstractThe mitochondrial genomes (mitogenomes) of plants are known to incorporate and accumulate DNA from intra- and extracellular donors. Despite the intimate relationships formed between flowing plants (angiosperms) and fungi, lengthy fungal-like sequence has not been identified in angiosperm mitogenomes to date. Here, we present multiple lines of evidence documenting horizontal gene transfer (HGT) between the mitogenomes of fungi and the ancestors of the orchids, plants that are obligate parasites of fungi during their early development. We show that the ancestor of the orchids acquired an ∼270-bp fungal mitogenomic region containing three transfer RNA genes. We propose that the short HGT was later replaced by a second HGT event transferring &gt;8 kb and 14 genes from a fungal mitogenome to that of the ancestor of the largest orchid subfamily, Epidendroideae. Our results represent the first evidence of genomic-scale HGT between fungal and angiosperm mitogenomes and demonstrate that the length intergenic spacer regions of angiosperm mitogenomes can effectively fossilize the genomic remains of ancient, nonplant organisms.
Exploration of intramolecular split G-quadruplex and its analytical applications
Authors: Lv Mengmeng , Guo Yuchun , Ren Jiangtao , Wang Erkang 
Published: August 2019

Abstract
Distinct from intermolecular split G-quadruplex (Inter-SG), intramolecular split G-quadruplex (Intra-SG) which could be generated in a DNA spacer-inserted G-quadruplex strand has not been systematically explored. Not only is it essential for the purpose of simplicity of DNA-based bioanalytical applications, but also it will give us hints how to design split G-quadruplex-based system. Herein, comprehensive information is provided about influences of spacer length and split mode on the formation of Intra-SG, how to adjust its thermodynamic stability, and selection of optimal Intra-SG for bioanalysis. For instances, non-classical Intra-SG (e.g. 2:10, 4:8 and 5:7) displays lower stability than classical split strands (3:9, 6:6 and 9:3), which is closely related to integrity of consecutive guanine tract; as compared to regular Intra-SG structures, single-thymine capped ones have reduced melting temperature, providing an effective approach to adjustment of stability. It is believed that the disclosed rules in this study will contribute to the effective application of split G-quadruplex in the field of DNA technology in the future.
Kinetic and subcellular analysis of PS-ASO/protein interactions with P54nrb and RNase H1
Authors: Vickers Timothy A , Rahdar Meghdad , Prakash Thazha P , Crooke Stanley T 
Published: August 2019

Abstract
The rapid RNase H1-dependent mislocalization of heterodimer proteins P54nrb and PSF to nucleoli is an early event in the pathway that explains the effects of most toxic phosphorothioate ASOs (PS-ASOs). Using a recently developed NanoLuciferace (NLuc)-based structural complementation reporter system which allows us to observe ASO/protein interactions in real time in live cells, we have determined that safe and toxic PS-ASOs associate with these proteins with kinetics and impact on subcellular localization that differ. Toxic PS-ASOs interact in a complex that includes RNase H1, P54nrb and PSF; but RNase H1/P54nrb complexes were observed in only the cells treated with toxic, but not safe PS-ASOs. In addition, experiments performed in vitro suggest that RNA is also a required component of the complex. The protein–protein interaction between P54nrb and RNase H1 requires the spacer region of RNAse H1, while the P54nrb core domains are required for association with RNase H1. In addition, we have determined that PS-ASOs bind P54nrb via RRM1 and RRM2, while they bind RNase H1 primarily via the hybrid binding domain, however catalytic domain interactions also contribute to overall affinity. These ASO–protein interactions are highly influenced by the chemistry of the PS-ASO binding environment, however little correlation between affinity for specific proteins and PS-ASO toxicity was observed.
Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice
Authors: Li Jin-Jing , Lin Xiang , Tang Cheng , Lu Ying-Qian , Hu Xinde , Zuo Erwei , Li He , Ying Wenqin , Sun Yidi , Lai Lu-Lu , Chen Hai-Zhu , Guo Xin-Xin , Zhang Qi-Jie , Wu Shuang , Zhou Changyang , Shen Xiaowen , Wang Qifang , Lin Min-Ting , Ma Li-Xiang , Wang Ning , Krainer Adrian R , Shi Linyu , Yang Hui , Chen Wan-Jin 
Published: August 2019

Abstract
We here report a genome-editing strategy to correct spinal muscular atrophy (SMA). Rather than directly targeting the pathogenic exonic mutations, our strategy employed Cas9 and guide-sgRNA for the targeted disruption of intronic splicing-regulatory elements. We disrupted intronic splicing silencers (ISSs, including ISS-N1 and ISS + 100) of survival motor neuron (SMN) 2, a key modifier gene of SMA, to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs. Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration. Furthermore, co-injection of Cas9 mRNA from Streptococcus pyogenes (SpCas9) or Cas9 from Staphylococcus aureus (SaCas9) alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56% and 100% of severe SMA transgenic mice (Smn−/−, SMN2tg/−). The median survival of the resulting mice was extended to &gt;400 days. Collectively, our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases.
Interest in Illicit Purchase of Cigarettes Under a Very Low Nicotine Content Product Standard
Authors: Hall Marissa G , Byron M Justin , Brewer Noel T , Noar Seth M , Ribisl Kurt M 
Published: August 2019

Abstract

Significance
The US Food and Drug Administration (FDA) is considering a very low nicotine content (VLNC) product standard to substantially reduce nicotine in cigarettes. We examined whether learning about a potential VLNC standard increased smokers’ interest in illicit purchases of cigarettes with regular nicotine content if such a standard were adopted.

Methods
Participants were a national convenience sample of 1712 US adult smokers. In an online experiment, we randomly assigned smokers to view information about a new VLNC standard (experimental condition) or no information (control condition). The experimental condition explained that a VLNC standard would remove 95% of the nicotine in cigarettes and would require stores to only sell VLNC cigarettes. Then, the survey assessed smokers’ interest in purchasing regular cigarettes from three illicit sources.

Results
Smokers who learned about the VLNC standard were more likely to be very or extremely interested in purchasing regular cigarettes illicitly from a Web site compared to smokers in the control group (24% vs. 16%, p &lt; .001). They were also more interested in illicitly buying cigarettes from a street vendor (19% vs. 13%, p &lt; .001) and a store on an Indian reservation (28% vs. 22%, p &lt; .05), compared to the control. The impact of learning about the VLNC standard on interest in illicit purchases did not differ by smoking frequency or current e-cigarette use.

Conclusions
A VLNC standard could increase smokers’ interest in illicit purchases of regular nicotine cigarettes. To prevent VLNC-induced illicit trade from undermining public health, FDA should consider proven measures such as track and trace for these products.

Implications
Little is known about how a VLNC cigarette standard would affect consumer interest in regular content cigarettes purchased from illicit sources (eg, the Internet). We found that smokers informed about a potential VLNC product standard had greater interest in illicit cigarette purchases, compared to controls. This suggests the importance of proactive measures accompanying a VLNC standard, such as track-and-trace cigarette packaging regulations and communication campaigns, in order to maximize the standard’s public health impact.
Nicotine Reduction in Cigarettes: Literature Review and Gap Analysis
Authors: Berman Micah L , Glasser Allison M 
Published: August 2019

Abstract

Background
The US Food and Drug Administration (FDA) is considering reducing nicotine levels in cigarettes to “minimally or non-addictive levels.” However, important research gaps remain, and the FDA must determine when the available research is sufficient to support moving forward.

Methods
The authors conducted a systematic review of research articles in PubMed relating to nicotine reduction. Building on a review of risk assessment best practices, the authors also developed a risk assessment framework for tobacco regulation and used it to guide a gap analysis of nicotine reduction research.

Results
The final sample consisted of 78 articles. The majority examined either nicotine dependence on very low nicotine cigarettes (VLNCs) or markers of potential health effects of using VLNCs. One-third of the identified articles reported results from four large randomized controlled trials (RCTs). While these studies report promising results and suggest that a nicotine reduction rule would be a powerful tool to reduce cigarette smoking, our gap analysis suggests that there is a need for studies that better reflect the use and availability of a wide range of tobacco/nicotine products and the potential for dual- or multi-product use.

Conclusion
The current body of research on nicotine reduction is weighted towards RCTs, which is appropriate for a policy that has not yet been implemented anywhere in the world. The FDA must consider a wide range of factors that may impact a product standard’s public health impact, including those difficult to assess in RCTs, such as a nicotine reduction rule’s impact on smoking initiation and relapse.

Implications
This systematic review presents a gap analysis based on a risk assessment framework to help identify remaining research priorities to inform FDA’s potential product standard to reduce nicotine levels in cigarettes. Quickly addressing those gaps would support the FDA’s effort to develop a nicotine reduction product standard that will be effective and withstand legal challenges.
Identification of Policy Priorities to Address the Burden of Smokeless Tobacco in Pakistan: A Multimethod Analysis
Authors: Siddiqi Kamran , Islam Ziauddin , Khan Zohaib , Siddiqui Faraz , Mishu Masuma , Dogar Omara , Shah Vandana , Khan Javaid , Pokhrel Subhash , Iqbal Romaina , Bauld Linda , Sheikh Aziz , Grugel Jean ,   ASTRA
Published: August 2019

Abstract

Introduction
We assessed the magnitude of smokeless tobacco (ST) use in Pakistan and identified policy gaps to help ascertain short-, medium-, and long-term priorities. We then elicited stakeholders’ views as to which of these identified priorities are most important.

Methods
In a multimethod study, we: analyzed Global Tobacco Surveillance System data sets to estimate ST consumption and disease burden; conducted a documentary review to identify gaps in policies to control ST in comparison with smoking; elicited stakeholders’ views in an interactive workshop to identify a set of policy options available to address ST burden in Pakistan; and ranked policy priorities using a postevent survey.

Results
Among all tobacco users in Pakistan (n = 24 million), one-third of men and two-thirds of women consume ST. In 2017, its use led to an estimated 18 711 deaths due to cancer and ischemic heart disease. Compared to smoking, policies to control ST lag behind significantly. Priority areas for ST policies included: banning ST sale to and by minors, advocacy campaigns, introduction of licensing, levying taxes on ST, and standardizing ST packaging. A clear commitment to close cooperation between state actors and stakeholder groups is needed to create a climate of support and information for effective policy making.

Conclusions
Smokeless tobacco control in Pakistan should focus on four key policy instruments: legislation, education, fiscal policies, and quit support. More research into the effectiveness of such policies is also needed.

Implications
A number of opportunities to improve ST regulation in Pakistan were identified. Among these, immediate priorities include banning ST sale to and by minors, mobilizing advocacy campaign, introduction of licensing through the 1958 Tobacco Vendors Act, levying taxes on ST, and standardizing ST packaging.
Smoking Cessation, Weight Gain, and the Trajectory of Estimated Risk of Coronary Heart Disease: 8-Year Follow-up From a Prospective Cohort Study
Authors: Chen Sanmei , Kawasaki Yohei , Hu Huanhuan , Kuwahara Keisuke , Yamamoto Makoto , Uehara Akihiko , Honda Toru , Yamamoto Shuichiro , Nakagawa Tohru , Miyamoto Toshiaki , Okazaki Hiroko , Hori Ai , Shimizu Makiko , Murakami Taizo , Kochi Takeshi , Eguchi Masafumi , Imai Teppei , Nishihara Akiko , Tomita Kentaro , Akter Shamima , Kabe Isamu , Mizoue Tetsuya , Dohi Seitaro 
Published: August 2019

Abstract

Introduction
The effect of weight gain following smoking cessation on cardiovascular risks is unclear. We aimed to prospectively investigate the association of weight gain following smoking cessation with the trajectory of estimated risks of coronary heart disease (CHD).

Methods
In a cohort of 18 562 Japanese male employees aged 30–64 years and initially free of cardiovascular diseases, participants were exclusively grouped into sustained smokers, quitters with weight gain (body weight increase ≥5%), quitters without weight gain (body weight increase &lt;5% or weight loss), and never smokers. Global 10-year CHD risk was annually estimated by using a well-validated prediction model for the Japanese population. Linear mixed models and piecewise linear mixed models were used to compare changes in the estimated 10-year CHD risk by smoking status and weight change following smoking cessation.

Results
During a maximum of 8-year follow-up, both quitters with and without weight gain had a substantially decreased level of estimated 10-year CHD risk after quitting smoking, compared with sustained smokers (all ps for mean differences &lt; .001). The estimated 10-year CHD risk within the first year after cessation decreased more rapidly in quitters without weight gain than in quitters with weight gain (change rate [95% confidence interval, CI] −0.90 [−1.04 to −0.75] vs. −0.40 [−0.60 to −0.19] % per year, p &lt; .0001). Thereafter, the estimated 10-year CHD risk in both groups increased at similar rates (change rate [95% CI] −0.07 [−0.21 to 0.07] vs. 0.11 [−0.09 to 0.30] % per year, p = .16, from year 1 to year 2; and 0.10 [0.05 to 0.15] vs. 0.11 [0.04 to 0.18] % per year, p = .80, from year 2 to year 8).

Conclusions
In this population of middle-aged, Japanese male workers, smoking cessation greatly reduces the estimated 10-year risk of CHD. However, weight gain weakens the beneficial effect of quitting smoking in a temporary and limited fashion.

Implications
To the best of our knowledge, this study is the first to examine the effect of weight gain following smoking cessation on the trajectory of the absolute risk of CHD. Our data imply that the benefits of cessation for reducing the absolute risk of CHD outweigh the potential risk increase due to weight gain, and suggest that in order to maximize the beneficial effects of quitting smoking, interventions to control post-cessation weight gain might be warranted.
Mondor’s disease after extensive training with Nordic walking
Authors: Hemsing Anette Lodvir , Reikvam Håkon 
Published: August 2019

Abstract
We here present a case of a 59-year-old man with Mondor’s disease, thrombophlebitis of the superficial veins of the anterior chest wall. This occurred after the patient had initiated extensive training with walking poles, Nordic walking, probably predisposing to the thrombosis. Underlying disease was ruled out, and the treatment was symptomatic. Physicians should be aware of this condition in patients performing extensive upper body workout.
Prognostic value of comorbidity indices and lung diseases in early rheumatoid arthritis: a UK population-based study
Authors: Nikiphorou Elena , de Lusignan Simon , Mallen Christian , Roberts Jacqueline , Khavandi Kaivan , Bedarida Gabriella , Buckley Christopher D , Galloway James , Raza Karim 
Published: August 2019

Abstract

Objectives
We assessed comorbidity burden in people with RA at diagnosis and early disease (3 years) and its association with early mortality and joint destruction. The association between lung disease and mortality in RA is not well studied; we also explored this relationship.

Methods
From a contemporary UK-based population (n = 1, 475 762) we identified a cohort with incident RA (n = 6591). The prevalence of comorbidities at diagnosis of RA and at 3 years was compared with age- and gender-matched controls (n = 6591). In individuals with RA we assessed the prognostic value of the Charlson Comorbidity Index and Rheumatic Disease Comorbidity Index calculated at diagnosis for all-cause mortality and joint destruction (with joint surgery as a surrogate marker). We separately evaluated the association between individual lung diseases [chronic obstructive pulmonary disease (COPD), asthma and interstitial lung disease] and mortality.

Results
Respiratory disease, cardiovascular disease, stroke, diabetes, previous fracture and depression were more common (P &lt; 0.05) in patients with RA at diagnosis than controls. Comorbidity (assessed using RDCI) was associated with all-cause mortality in RA [adjusted hazard ratio (HR) 1.26, 95% CI 1.00–1.60]. There was no association with joint destruction. COPD, but not asthma, was associated with mortality (COPD HR 2.84, 95% CI 1.13–7.12).

Conclusion
There is an excess burden of comorbidity at diagnosis of RA including COPD, asthma and interstitial lung disease. COPD is a major predictor of early mortality in early RA. Early assessment of comorbidity including lung disease should form part of the routine management of RA patients.