bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endothelin receptor Aa regulates proliferation and differentiation of Erb-dependant pigment progenitors in zebrafish
Authors: Camargo-Sosa Karen , Colanesi Sarah , Müller Jeanette , Schulte-Merker Stefan , Stemple Derek , Elizabeth Patton E. , Kelsh Robert N. 
Published: April 2018

AbstractSkin pigment patterns are important, being under strong selection for multiple roles including camouflage and UV protection. Pigment cells underlying these patterns form from adult pigment stem cells (APSCs). In zebrafish, APSCs derive from embryonic neural crest cells, but sit dormant until activated to produce pigment cells during metamorphosis. The APSCs are set-aside in an ErbB signaling dependent manner, but the mechanism maintaining quiescence until metamorphosis remains unknown. Mutants for a pigment pattern gene,parade, exhibit ectopic pigment cells localised to the ventral trunk. We show thatparadeencodes Endothelin receptor Aa, expressed in the blood vessels. Using chemical genetics, coupled with analysis of cell fate studies, we show that the ectopic pigment cells derive from APSCs. We propose that a novel population of APSCs exists in association with medial blood vessels, and that their quiescence is dependent upon Endothelin-dependent factors expressed by the blood vessels.Lay AbstractPigment patterns are crucial for the many aspects of animal biology, for example, providing camouflage, enabling mate selection and protecting against UV irradiation. These patterns are generated by one or more pigment cell-types, localised in the skin, but derived from specialised stem cells (adult pigment stem cells, APSCs). In mammals, such as humans, but also in birds and fish, these APSCs derive from a transient population of multipotent progenitor cells, the neural crest. Formation of the adult pigment pattern is perhaps best studied in the zebrafish, where the adult pigment pattern is formed during a metamorphosis beginning around 21 days of development. The APSCs are set-aside in the embryo around 1 day of development, but then remain inactive until that metamorphosis, when they become activated to produce the adult pigment cells. We know something of how the cells are set-aside, but what signals maintain them in an inactive state is a mystery. Here we study a zebrafish mutant, calledparade, which shows ectopic pigment cells in the embryo. We clone theparadegene, identifying it asednraaencoding a component of a cell-cell communication process, which is expressed in blood vessels. By characterising the changes in the neural crest and in the pigment cells formed, and by combining this with an innovative assay identifying drugs that prevent the ectopic cells from forming, we deduce that the ectopic cells in the larva derive from precocious activation of APSCs to form pigment cells. We propose that a novel population of APSCs are associated with the blood vessels, that these are held in a quiescent state by signals coming from these vessels, and that these signals depend uponednraa. Together this opens up an exciting opportunity to identify the signals maintaining APSC quiescence in zebrafish.
Imaging beyond the super-resolution limits using ultrastructure expansion microscopy (UltraExM)
Authors: Gambarotto D. , Zwettler F. U. , Cernohorska M. , Fortun D. , Borgers S. , Heine J. , Schloetel J. G. , Reuss M. , Unser M. , Boyden E. S. , Sauer M. , Hamel V. , Guichard P. 
Published: April 2018

ABSTRACTFor decades, electron microscopy (EM) was the only method able to reveal the ultrastructure of cellular organelles and molecular complexes because of the diffraction limit of optical microscopy. In recent past, the emergence of superresolution fluorescence microscopy enabled the visualization of cellular structures with so far unmatched spatial resolution approaching virtually molecular dimensions. Despite these technological advances, currently super-resolution microscopy does not permit the same resolution level as provided by electron microscopy, impeding the attribution of a protein to an ultrastructural element. Here, we report a novel method of near-native expansion microscopy (UltraExM), enabling the visualization of preserved ultrastructures of macromolecular assemblies with subdiffraction-resolution by standard optical microscopy. UltraExM revealed for the first time the ultrastructural localization of tubulin glutamylation in centrioles. Combined with super-resolution microscopy, UltraExM unveiled the centriolar chirality, an ultrastructural signature, which was only visualizable by electron microscopy.
Cell-cell contact dictates life or death decisions following CD95 activation in cancer
Authors: Gülcüler Balta Gülce S. , Monzel Cornelia , Kleber Susanne , Beaudouin Joel , Kaindl Thomas , Thiemann Meinolf , Wirtz Christian R. , Tanaka Motomu , Martin-Villalba Ana 
Published: April 2018

AbstractCancer cells react to CD95 activation with either apoptotic or tumorigenic responses. Yet, the determinants of these two antithetic reactions are fundamentally not understood. Here, we show that pre-confined CD95L molecules activate apoptosis of cancer cells in-vitro. For particular CD95L pre-confinement, apoptosis activation is most efficient. Surprisingly, in tumor models, the same pre-confinement yields enhanced proliferation of cancer cells. This shift is rooted in cell-cell interactions, as proliferation was also observed in tumorspheres in-vitro. Indeed, proliferation required death-domain tyrosine phosphorylation of CD95 that was facilitated by cell-cell contacts, whereas decreasing the levels of global tyrosine kinase activity favored apoptosis. Altogether, the response to CD95 activation is cell context-dependent and tunable by CD95L pre-confinement, thereby opening therapeutic opportunities in cancer.One Sentence SummaryCell-cell contact tunes tyrosine-kinase activity thereby dictating life or death upon CD95 activation by pre-confined CD95L.
Quantifying multi-layered expression regulation in response to stress of the endoplasmic reticulum
Authors: Rendleman Justin , Cheng Zhe , Maity Shuvadeep , Kastelic Nicolai , Munschauer Mathias , Allgoewer Kristina , Teo Guoshou , Zhang Yun Bin , Lei Amy , Parker Brian , Landthaler Markus , Freeberg Lindsay , Kuersten Scott , Choi Hyungwon , Vogel Christine 
Published: April 2018

AbstractThe mammalian response to endoplasmic reticulum (ER) stress dynamically affects all layers of gene expression regulation. We quantified transcript and protein abundance along with footprints of ribosomes and non-ribosomal proteins for thousands of genes in cervical cancer cells responding to treatment with tunicamycin or hydrogen peroxide over an eight hour time course. We identify shared and stress-specific significant regulatory events at the transcriptional and post-transcriptional level and at different phases of the experiment. ER stress regulators increase transcription and translation at different times supporting an adaptive response. ER stress also induces translation of genes from serine biosynthesis and one-carbon metabolism indicating a shift in energy production. Discordant regulation of DNA repair genes suggests transcriptional priming in which delayed translation fine-tunes the early change in the transcriptome. Finally, case studies on stress-dependent alternative splicing and protein-mRNA binding demonstrate the ability of this resource to generate hypotheses for new regulatory mechanisms.
B7-H1(PD-L1) confers chemoresistance through ERK and p38 MAPK pathway in tumor cells
Authors: Wu Xiaosheng , Li Yanli , Liu Xin , Cao Siyu , Harrington Susan M. , Chen Chunhua , Mansfiled Aaron S. , Dronca Roxana S. , Park Sean S. , Yan Yiyi , Kwon Eugene D. , Wang Liewei , Ling Kun , Dong Haidong 
Published: April 2018

ABSTRACTDevelopment of resistance to chemotherapy and immunotherapy is a major obstacle in extending the survival of patients with cancer. Although several molecular mechanisms have been identified that can contribute to chemoresistance, the role of immune checkpoint molecules in tumor chemoresistance remains underestimated. It has been recently observed that overexpression of B7-H1(PD-L1) confers chemoresistance in human cancers, however the underlying mechanisms are unclear. Here we show that the development of chemoresistance depends on the increased activation of ERK pathway in tumor cells overexpressing B7-H1. Conversely, B7-H1 deficiency renders tumor cells susceptible to chemotherapy in a cell-context dependent manner through activation of the p38 MAPK pathway. B7-H1 in tumor cells associates with the catalytic subunit of a DNA-dependent serine / threonine protein kinase (DNA-PKcs). DNA-PKcs is required for the activation of ERK or p38 MAPK in tumors expressing B7-H1, but not in B7-H1 negative or B7-H1 deficient tumors. Ligation of B7-H1 by anti-B7-H1 monoclonal antibody (H1A) increased the sensitivity of human triple negative breast tumor cells to cisplatin therapy in vivo. Our results suggest that B7-H1(PD-L1) expression in cancer cells modifies their chemosensitivity towards certain drugs and targeting B7-H1 intracellular signaling pathway is a new way to overcome cancer chemoresistance.
Sucrose or sucrose and caffeine differentially impact memory and anxiety-like behaviours, and alter hippocampal parvalbumin and doublecortin
Authors: Xu Tanya J. , Reichelt Amy C. 
Published: April 2018

AbstractCaffeinated sugar-sweetened “energy” drinks are a subset of soft drinks that are popular among young people worldwide. High sucrose diets impair cognition and alter aspects of emotional behaviour in rats, however, little is known about sucrose combined with caffeine. Rats were allocated to 2h/day 10% sucrose (Suc), 10% sucrose plus 0.04% caffeine (CafSuc) or control (water) conditions. The addition of caffeine to sucrose appeared to increase the rewarding aspect of sucrose, as the CafSuc group consumed more solution than the Suc group. After 14 days of intermittent Suc or CafSuc access, anxiety was assessed in the elevated plus maze (EPM) prior to their daily solution access, whereby CafSuc and Suc rats spent more time in the closed arms, indicative of increased anxiety. Following daily solution access, CafSuc, but not Suc, rats showed reduced anxiety-like behaviour in the open-field. Control and CafSuc rats displayed intact place and long-term object memory, while Suc showed impaired memory performance. Sucrose reduced parvalbumin immunoreactivity in the hippocampus, but no differences were observed between Control and CafSuc conditions. Parvalbumin reactivity in the basolateral amygdala did not differ between conditions. Reduced doublecortin immunoreactivity in the dentate gyrus relative to controls was seen in the CafSuc, but not Suc, treatment condition. These findings indicate that the addition of caffeine to sucrose attenuates cognitive deficits. However, the addition of caffeine to sucrose evokes anxiety-like responses under certain testing conditions, suggesting that frequent consumption of caffeinated energy drinks may promote emotional alterations and brain changes compared to standard soft drinks.
Advertising strategy and its effectiveness on consumer online search in a defaming product-harm crisis
Authors: Jang Sungha , Kim Jinsoo , Song Reo , Kim Ho 
Published: April 2018

PurposeActual product-harm crises pose significant challenges to firms, but so can defaming product-harm crises, which are defined as crises caused by false or malicious rumors made by consumers or competing firms. Unlike typical product-harm crises, in defaming product-harm crises, the truth often emerges only after substantial damage has been done to the victim firm. Thus, crisis management strategies in these two cases may be different. The paper aims to discuss these issues.Design/methodology/approachUsing a defaming product-harm crisis that involved two competing firms, this paper examines how the firms changed their advertising strategies and how the changes affected consumers’ online search behavior regarding the two firms.FindingsThe analyses show that after the crisis, the offending firm sensitively reacted to its own and the victim firm’s advertising levels, but the victim firm did not react to the offending firm’s advertising as it had previously. The effectiveness of advertising on consumers’ online search weakened for both firms after the crisis.Originality/valueThe paper provides a new insight about marketing strategies and their effectiveness in the product-harm crisis literature.
Synthesizing moderate upscale restaurant patrons’ dining experiences
Authors: Clemes Michael Daniel , Mohi Zurinawati , Li Xuedong , Hu Baiding 
Published: April 2018

PurposeThe purpose of this paper is to develop and test a comprehensive hierarchical model of the interrelationships among five higher order marketing constructs (service quality, customer satisfaction, perceived value, restaurant image and behavioural intentions) for moderate upscale restaurants in Malaysia. A third order conceptualisation of service quality is also included in the empirical analysis.Design/methodology/approachThe data were analysed using exploratory factor analysis, confirmatory factor analysis and structural equations.FindingsService quality, customer satisfaction and restaurant image all have a strong, positive impact on the behavioural intentions of moderate upscale restaurant patrons. Service quality, perceived value and restaurant image are also important determinants of customer satisfaction. Behavioural intentions to re-patronise a restaurant and recommend it to friends are influenced more strongly by restaurant image than by customer satisfaction. Three primary dimensions and ten pertaining sub-dimensions are modelled in the third order conceptualisation of service quality. The primary and sub-dimensions also vary in importance amongst the customers in the sampling frame.Originality/valueThis is the first empirical research that develops and tests a comprehensive hierarchical model for moderate upscale restaurants to provide a complete and integrative analysis of a service setting. The interrelationships among service quality, customer satisfaction, perceived value restaurant image and behavioural intentions are assessed. A third order conceptualisations of service quality is also included in the modelling framework.
Research output on terrorism by India and Pakistan: a bibliometric study
Authors: Loan Fayaz Ahmad , Shah Refhat-un-nisa 
Published: April 2018

PurposeThis study aims to explore the scholarly literature published on “Terrorism” by India and Pakistan and has an in-depth bibliometric analysis of its various facets.Design/methodology/approachData for the study were harvested from the “Web of Science” one of the widely used and trusted indexing and abstracting source used by research scholars all over the world. The search was confined to the literature published on “Terrorism” by India and Pakistan. A total of 125 results were retrieved and out of them 105 were found relevant. The data were later analyzed on different parameters.FindingsIndia and Pakistan have started to conduct and publish research on “Terrorism” since 1990. However, only five articles were published till 2000. Presently, out of total 105 publications, India contributed 57 (54 per cent) and Pakistan contributed 48 (46 per cent) articles on Terrorism. Majority of the articles (93 per cent) were contributed by the individual researchers without any financial support from the funding agencies. The authorship pattern of the articles revealed that 40.95 per cent (43) articles were contributed by single author, whereas 59.05 per cent (62) were authored in collaboration. In terms of research collaboration, Pakistani authors collaborated with authors of six nations of the world, whereas Indian authors collaborated only with researchers of three countries. Most of the research articles were published from the authors affiliated with universities and other higher educational institutions (64.76 per cent), followed by colleges (12.38 per cent) and other government departments. Most of the articles were published during last five years from 2011-2015 (52.38 per cent), followed by 2006-2010 (28.57 per cent). These articles were published in more than 80 journals and the majority were published from England (36, 34.29 per cent) followed by United States of America (26, 24.76 per cent) and Netherlands (13, 12.38 per cent) simultaneously.Research limitations/implicationsThe findings of the study need to be cautioned as the data were harvested from “Web of Science” only.Practical implicationsThe paper is useful for politicians, defense ministries, academicians, researchers and many other interested agencies such as human rights organizations or activists.Originality/valueThis is the first paper providing information on research output of India and Pakistan on Terrorism and will serve as bed rock for future research in the field.
Social media as a vehicle for user engagement with local history
Authors: Hood Caroline , Reid Peter 
Published: April 2018

Purpose
The purpose of this paper is to examine issues associated with user engagement on social media with local history in the North East of Scotland and to focus on a case study of the Buckie and District Fishing Heritage Society, a small but very successful and professionally-run community-based local heritage organisation.

Design/methodology/approach
A qualitative approach using photo elicitation on social media was deployed in conjunction with analysis of the user interactions and the reach insights provided by Facebook to the page manager. Additionally, a focus group was used.

Findings
The research, although focussed on an individual case study, offers significant lessons which are more widely applicable in the local history and cultural heritage social media domain. Key aspects include user engagement and how digital storytelling can assist in the documentation of local communities ultimately contributing to local history research and the broader cultural memory. The significance of the image and the photo elicitation methodology is also explored.

Social implications
The research demonstrates new opportunities for engaging users and displaying historical content that can be successfully exploited by community heritage organisations. These are themes which will be developed within the paper. The research also demonstrates the value of photo elicitation in both historical and wider information science fields as a means of obtaining in-depth quality engagement and interaction with users and communities.

Originality/value
The research explored the underutilised method of photo elicitation in a local history context with a community possessed of a strong sense of local identity. In addition to exploring the benefits of this method, it presents transferable lessons for how small, community-based history and heritage organisation can engage effectively with their audience.
Diffusion of open source integrated library systems in academic libraries in Africa
Authors: Ponelis Shana Rachel , Adoma Philliam 
Published: April 2018

Purpose
Libraries globally automate their operations and services using integrated library systems (ILS) to increase operational efficiency and meet the ever-evolving demands of their users. Open source ILS (OS ILS) has become more popular globally. The purpose of this paper is to better understand the diffusion of OS ILS in academic libraries in Uganda.

Design/methodology/approach
Rogers’ diffusion of innovation (DoI) supplemented by the fit-viability theory was used as a theoretical framework. A questionnaire was developed based on extant literature and distributed electronically to representative members of a Ugandan university library association.

Findings
The diffusion of OS ILS in Ugandan academic libraries approximates the S-curve expected based on DoI. Ugandan academic libraries are adopting OS ILS for more flexibility to meet changing needs at what is perceived as an affordable cost but not all are fully satisfied. Koha is the most adopted OS ILS and is also being considered by all libraries without any ILS or a proprietary ILS. The information and communication technology (ICT) infrastructure, organizational procurement policies and national procurement legislation, human resource capacity and limited finances are barriers to diffusion. The total cost of ownership and technical skills required are of particular concern for OS ILS.

Research limitations/implications
The research was limited to a single African country and the recommendations may not be transferable to other African countries. Future research can expand the survey, the countries studied and/or address the methodological limitations of this study.

Practical implications
When embarking upon library automation using OS ILS or migrating to an OS ILS solution libraries should consider their ICT infrastructure, local support community, available training and be realistic about the costs. Local library associations should provide guidance on OS ILS selection, ongoing training, and opportunities for knowledge sharing. LIS schools should consider expanding their curriculum to include library automation and, in Uganda incorporate training on Koha as OS ILS. Libraries and library associations should advocate to reduce restrictive organizational procurement policies and national procurement legislation.

Originality/value
Studies on country-level diffusion of ILS are comparatively few, particularly in African countries. This is the first country-level study of OS ILS diffusion in Uganda. This study can positively impact future patterns of diffusion for optimal deployment of OS ILS software by informing academic libraries, university management, library association, LIS schools, and policymakers in Uganda and across the African continent, and encouraging academics and researchers to teach and study library automation using OS ILS.
Nutritive value andtransfatty acid content of fast foods in Qena city, Egypt
Authors: Sadek Mustafa Abdel-samie , Daoud Jehan Ragab , Ahmed Hussein Youssef , Mosaad Gamal Mohamed 
Published: April 2018

PurposeThis study aims to investigate the nutrient composition and cholesterol content of most popular fast foods sold in Qena city, Egypt with reference to theirtransfatty acids contents.Design/methodology/approachTotal 80 samples of fast foods were collected from various fast food restaurants in Qena city, Egypt. The samples were investigated for their nutritive quality by measuring moisture, protein, fat, ash, carbohydrate and energy content as described by the association of analytical chemists. The cholesterol content was determined using a spectrophotometer, whiletransfatty acids were analyzed by gas chromatography using standard methods.FindingsThe study revealed a wide variation in the composition and nutritive value. On a fresh weight basis, moisture, protein, fat, carbohydrate and ash ranged from 45.9-55.0 (P< 0.001), 15.0-22.3 (P< 0.001), 13.1-15.7 (P= 0.034), 6.0-16.2 (P< 0.001) and 2.2-3.5 (P< 0.001), %w/w, respectively. The fast foods were very energy dense with calorie content varying from 236.5-281.1 (P< 0.001), Kcal/100 g. The mean values of cholesterol were between 15-17 mg/100 g (P= 0.398). The findings showed that fast foods had high totaltransfatty acids content exceeding the Danish legal limit of 2 g/100 g of fat being in the range of 3.5 to 11.3 g per 100 g fat (P= 0.379). Elaidic acid is the predominant trans-isomer in industrially producedtransfatty acids. The concentrations of elaidic acid were high in all samples examined (P= 0.942).Originality/valueThis study has established the fact that fast foods are associated with high total energy intake, high intake of fat, trans fat and a higher proportion of calories being derived from total fat and trans-fat. Therefore, it is recommended to limit the intake of fast food.