bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunotherapeutic modification of Escherichia coli peritonitis and bacteremia by Iris kashmiriana baker
Authors: Wagay Javeed Iqbal , Jain Kirti 
Published: January 2019

A larger number of medicinal plants and their purified constituents have been shown beneficial therapeutic potentials. We present here the protective effects of an Indian medicinal plant Iris Kashmiriana as compared to Ofloxacin in E. coli induced peritonitis. Iris Kashmiriana is one of an important member of family Iridaceae, locally known as Mazarmund in Kashmir. The plant has been widely used in traditional medicine and modern clinical preparations to treat cold, flu, malaria, toothache, cancer, bacterial, viral infections and bruise. Rats were pre-treated with 200 mg/kg and 400 mg/kg/bwt dose for 3 days and fourth day with E. coli (1×108 CFU/ml) strain and consecutively 3 days treatment. Mortality was monitored for 14 days. After the death of rats or completion of the experiment rats were sacrifice and kidney were used for our protocol. Colonies were count and statically analysis was done. Results showed dose dependent anti-microbial activity. Pretreatment of mice with Iris Kashmiriana improved bacterial clearance as well as improved phagocytic and intracellular bactericidal capacities of neutrophils. In the Ofloxacin treated mice although bacterial clearance was rapid, polymorph phagocytosis was depressed. Thus the results, obtained justify the traditional use of Iris Kashmiriana.
Keywords: Iris kashmiriana, E. coli induced peritonitis, Neutrophils, Ofloxacin.
Extraction, Qualitative and Quantitative Determination of Secondary Metabolites of Rumex Nepalensis Roots
Authors: Khan Azher Nawaz , Bhat Irm 
Published: January 2019

In the Indian ayurvedic system of medicine, Rumex nepalensis Spreng. (Polygonaceae) commonly known as Nepal Dock has wide-spectrum therapeutic potencies and is extensively used for centuries in traditional medicine systems. They act as a possible food supplement and are largely used in pharmaceutical industry. Extracts and metabolites from this plant exhibits pharmacological activities including anti-inflammatory, antioxidant, antibacterial, antifungal, antiviral, insecticidal, purgative, analesic, antipyretic, anti-algal, central nervous system depressant, genotoxic, wound healing and skeletal muscle relaxant activity. Due to its remarkable biological activities, it has the potential to act as a rich source of drug against life threatening diseases. The aim of the present study is to examine Rumex nepalensis roots for phytochemical profile. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of roots ethanolic extract was (1.658 mg/100mg), followed by flavonoids (1.048mg/100mg). The present study concluded that the crude extract of Rumex nepalensis is a potential source of various activates and this justifies its use in folkloric medicine.
Keywords: Rumex nepalensis, Qualitative analysis, Quantitative analysis, TPC, TFC, Folins Ciocalteau
In Vitro Clot Dissolving Activity of Carbomer Based Gel Containing Ethanolic Extract of Calotropis Gigantea Leaves
Authors: Alawa Prerna , Tenguria Mayank , Nilofey Vijayshree 
Published: January 2019

Blood clotting is an important and vital process that takes places in humans, animals and birds. At the same time blood clots sometimes considered an issue when developed in the circulatory system due to failure of hemostasis causes vascular blockage and leads to serious consequences in thrombolytic diseases such as acute myocardial or cerebral infarction which may cause death. Certain substances like Alteplase, anistreplase, streptokinase, urokinase, tissue plasminogen activator, Heparin and Aspirin are used as clot dissolving agents. Plants have also been proved to perform such activity. Calotropis gigantea L. is a traditional medicinal plant with several pharmacological properties belongs to the family Apocyanaceae of Asclepiadaceous was investigated for its efficacy for blood clot dissolving activity under in vitro conditions when incorporated into carbomer based gels. The ethanolic extract of the leaves of C. gigantea was prepare by soxhlation which upon analysis reported to contain alkaloids, glycosides, flavonoids, tannins, terpenoids and saponins as usual components as were reported in earlier investigations. There were 3 gels with ethanolic leaf extract C. gigantea with 1 mg, 0.5 mg and 0.25 mg per gram of 1% Carbopol-940 gel containing methyl paraben, glycerol and water. The two gels containing sterile distilled water and 0.1 mg per gram gel streptokinase enzyme served as –ve and positive controls respectively. The in vitro thrombolytic of clot dissolving activity war performed on goat blood collected from slaughter houses. From the results the percentage clot dissolving activity of gels containing 1 mg, 0.5 mg and 0.25 mg C. gigantea extract per gram were reported as 34%, 27% and 14% respectively considered significant when compared to the activity of standard streptokinase in the gel. The activity of these experimental gels describes the possibility of development of new safe, and non reactive topical anti-clot gel products using herbal or plant components like C. gigantea extracts.
Keywords: Calotropis gigantea L, Streptokinase, Thrombolytic activity, Carbopol gel, Biopharmaceutical.
Formulation and in-vitro evaluation of Glipizide (Anti diabetic drug) Liposphere
Authors: Saini Sarika , Mittal Aman 
Published: January 2019

Objective- The aim of the present study was to formulate and in- vitro study of glipizide liposphere by using melt dispersion technique.
Methods- Glipizide Liposphere system composed of paraffin wax, Stearic acid as lipid phase and sodium lauryl sulphate as surfactant. Glipizide lipospheres were prepared by using melt dispersion technique. Formulation of Glipizide was evaluated such as organoleptic properties, particle size, drug content, entrapment efficiency in-vitro study and stability of the lipospheres.
Result- The formation of glipizide lipospheres by using melt dispersion technique was done successfully. All the formulations have off- white in colour, characteristic odour and spherical shape. The formulation A4 has particle size 19.65 μm, drug content 84.93 %, entrapment efficiency 80.75 % and the percentage drug release was carried out by using USP type 2 dissolution apparatus in 6.8 pH phosphate buffer solution and drug release of glipizide lipospheres within 12 hrs was found to be 74.06 %.stability study of glipizide lipospheres revealed that the formulation was stable at 5oC ± 3oC.
Keywords- Lipospheres, Glipizide, Paraffin wax, Melt dispersion method, Dissolution Apparatus, Stability study
Wound healing prospective of pongamia glabra, piper nigrum and momordica charantia on albino rats using anemic burn wound model
Authors: Shukla Rashmi , Kashaw Varsha 
Published: January 2019

Objective: The objective of present work was to evaluate wound healing potential of pongamia glabra, piper nigrum and momordica charantia using as herbal ointment formulation on albino rats using burn wound model. All the plant has the rich source of alkaloid, glycoside, tannins, carbohydrates, saponins, flavonoids, proteins and amino acids content and were used as anti-diabetic, anti-inflammatory, antitumor, anti-malarial and having wound healing potential. Materials and Methods: Extract of all three plants has been separated by the Soxhlet extraction. Herbal ointment formulation has been prepared by mixing the extract of momordica charantia, pongamia glabra and piper nigrum with the wool fat and paraffin. Burn wound model has been utilized for the evaluation of wound healing potential. Histopathological evaluation has been also carried-out for the physical verification wound healing potential. Results and Conclusion: The studies on burn wound healing model reveals that all twelve groups showed decreased wound area on the time and there was no mortality observed in the course of study. Discussion: These studies have indicated that herbal ointment formulation of pongamia glabra, piper nigrum and momordica charantia has been utilized for wound healing and it is safer for topical application. No toxicity and mortality have been observed during the experimental tenure.
Keywords: Wound healing, momordica charantia, pongamia glabra, piper nigrum, burn wound model
Molecular Modeling of Cathepsin B protein in different Leishmania strains
Authors: Mahato Jayprakash 
Published: January 2019

Cathepsin B like cysteine proteases representing a major component of the lysosomal proteolytic repertoire plays an important role in intracellular protein degradation. Comparative models of cathepsin B (CatB) protein of six different Leishmania strains were developed using MODELLER. The modeled three-dimensional (3-D) structure has the correct stereochemistry as gauged from the Ramachandran plot and good 3-D structure compatibility as assessed by PROCHECK and the DOPE score (DS2.1, Accelrys). The modeled proteins were energy minimized and validated using standard dynamic cascade protocol (DS 2.1). Seven different disulfide bonding sites are predicted in CatB protein of Leishmania. Two domains were identified and different motifs are present in catB protein of Leishmania like aspargine glycosylation site, protein kinase phosphorylation site, Protein kinase C activation site, N-myristoylation site. Considering that cathepsin B is essential for survival of Leishmania, including for virulence to the mammalian host, it may be viewed as an attractive drug target.
Keyword: Molecular Modelling, Leishmania, Discover Studio, Protein Binding.
Formulation and characterization of choline fenofibrate sustained release capsule
Authors: Jain Nidhi , Jain Rohit , Dangi Surendra 
Published: January 2019

The drug ABT-335 (Fenofibricacid ) choline fenofibrate found to be useful in the treatment of dyslipidemia as compare with other fibrate. So the present investigation to developed mini-tablets to overcome the dose dumping problem by regular shape, uniformity, smooth surface excellent size and encapsulated in capsule to improve the efficacy and bioavailability of the drug. Different formulations were developed by utilizingcontrolling of the release rate and gel forming polymers like microcrystalline cellulose, hypromellose by dry granulation technique. Among all formulation SRM-3 having the drug release for longer period of time as compare to other formulation. SRM-3 was found to be stable during stability study for one month.
Keywords: ABT-335 (Fenofibric acid) choline fenofibrate, like microcrystalline cellulose, hypromellose, sustained release matrix tablet.
Microparticulate hot melt pallets technology: a review
Authors: Kokate Shekhar , Rachh Punit R 
Published: January 2019

Recent advances in novel drug delivery (NDDS) aims to enhance safety and efficacy of drug molecule by formulating a convenient dosage form for ease of administration and to achieve better patient compliance. Depending upon functionality, it is possible to design different dosages forms which fulfill the therapeutic needs of the patient with improved bioavailability of poorly water soluble drugs, taste masking and preparation of oral dispersible tablets, MUPS and multiple unit formulations. The hot-melt technology is one of the most commonly used method, is devoid of solvent use, solvent disposal, solvent evaporation and solvent treatment is not required.
Keywords: Multiple Unit Pallet Systems, Lipid, Solubility, Hot-Melt Technology, Bioavailability, Absorption of Drug.
Comparative analysis of different identification techniques for detection of mycobacterial species in clinical samples
Authors: Umrao Jyoti , Sivaperumal R 
Published: January 2019

Out of 906 cultures isolates, 263 (29.0%) were confirmed as NTM and 724 (71.0%) were identified as Mycobacterium tuberculosis complex. The diversity of NTM species was high and predominated by Mycobacterium abscessus (31.3%) followed by M. fortuitum (22%), M. intracellulare (13.6%), M. chelonae (9.1%), however, M. abscessus and M. fortuitum were the predominant species in both types of clinical isolates. Men (60.4%) and older patients aged greater than 55 years were the predominated risk group for NTM infection. Additionally, three HIV/AIDS cases (1.1%), as well as thirty five cases were found to be type two diabetes mellitus infected with non-tuberculous bacteria.
Keywords: NTM, Technique, BacT/Alert, Biochemical Test, Tuberculosis.
Formulation development and evaluation of medroxyprogesterone acetate injectable suspension
Authors: Sunitha Reddy M. , Samatha B. 
Published: January 2019

The aim of the present work is to develop sustained release parenteral drug delivery system of contraceptive drug i., e medroxyprogesterone acetate. The formulation was prepared by sterile combining of API and excipient powders by rapid stirring method. Different excipients used in the formulation are PEG 3350, Poloxamer -188, Polysorbate-80, Benzyl alcohol and Sodium chloride. The prepared parenteral suspension was evaluated for all official parameters like sedimentation volume, measurement of zeta potential, pH, viscosity, osmolality, dissolution, assay, particle size determination, and stability studies. The dissolution profile was found to be more when compared with the innovator formulation. Stability studies were also conducted at 25ºC / 60% RH and 40ºC / 75% RH conditions up to 3M.All the parameters evaluated are within the specified limits.
Keywords: Medroxy progesterone acetate, PEG 3350, Poloxamer-188, injectable suspension.
Expository aspect for action of Ksharaplota
Authors: Shekokar Anantkumar V. , Ukhalkar Vijay P. 
Published: January 2019

Kshara Karma or chemical cauterization described in Ayurveda as one of the Anushastras (Minimum Assess Surgery). It is considered superior in all types of Anushstra due to its efficacy in conditions where all other measures failed to cure diseases or surgical interventions. Healing of wounds and cuts is a very difficult if wound or cuts becomes worse and turned to Dushta Varna due to negligence and the inappropriate approaches of treatment. However wound healing is a natural defense mechanism of body but in complicated and severe cases there are need of therapeutic practices toboost healing mechanism of body. Father of Indian surgery Sushruta mentioned use of dressing materials such as; Pichu, Plota, Kawalika and Vikeshika for the management of Dushta Vrana, Plota used to clean & covers the Vrana very deeply and helps to removes debris of wounds effectively. Considering these all facts present article emphasized mode of action of Ksharaplota (Alkaline Medicated Gauze) for exploring its surgical significance with logical interventions.
Keywords: VranaShodhana, VranaRopana, DushtaVrana, Action of Ksharaplota, Alkaline Medicated Gauze.
Formulation and evaluation of prazosin hydrochloride loaded solid lipid nanoparticles
Authors: Bandgar Sandip Akaram , Dhavale Pranali , Patil Pravin , Shelake Sardar , Patil Shitalkumar 
Published: January 2019

Solid Lipid Nanoparticles (SLN) are rapidly developing field of nanotechnology with several potential application in drug delivery and research. Drugs having low aqueous solubility not only give low oral bioavailability but provide high inter-and intra subject variability. The purpose of the present study was to investigate the bioavailability enhancement of Prazosin Hydrochloride drug by formulating solid lipid nanoparticle. Prazosin Hydrochloride Drug is an antihypertensive drug with limited bioavailability so that solid lipid nanoparticle (SLN) is one of the approaches to improve bioavailability. SLN were prepared using glyceryl monostearate by hot homogenization followed by Solvent emulsification-ultrasonication. Prazosin Hydrochloride loaded SLN were characterized and optimized by parameters like particle size, zeta potential, XRD, DSC. Proposing Hydrochloride loaded SLN having the particle size 263.8±1.88 and entrapment efficiency 89.29±0.65% shows better bioavailability and optimum stability in studies. The SLN studies prepared using glyceryl mono stearate as a lipid and Polaxamer 407 as a polymer leads to improve bioavailability of the drug.
Keywords: Prazosin Hydrochloride, Solid Lipid Nanoparticles, Entrapment efficiency, DSC
Phytochemical screening of Lycopersicum esculentum Mill. treated with seaweed liquid fertilizer
Authors: Niranjanadevi M. , Sivakumar K. 
Published: January 2019

Premise of the study: The objective of the work is to manitor the phytochemicals present in methanolic extract of Lycopersicum esculentum after treatment with different concentrations of seaweed liquid fertilizer (SLF). Methods: In this study SLF treated L.esculentum were characterized through gas chromatography and mass spectroscopy to identify the phytochemicals constituents and functional groups of the compounds. Results: Preliminary phytochemical analysis revealed the presence of alkaloids, phenols, carbohydrates, saponins, glycosides, flavonoids, terpinoids. Among the thirty nine phytochemical constituents octa decanoic acid, stigmasterol, hexa decanoic acid and Vitamin E are relatively present in higher yield. The active phytochemical compounds and their constituents were identified with gas chromatography and mass spectrometry. The retention time, percentage of area, molecular weight and chemical formula of phytochemical compounds were determined with help of NIST08 and WILEY8 libraries. The functional groups identified from the spectrum techniques are alcohol, aldehyde, iso cyanides, alkyl compound and chloro compounds. Accordingly hexadecanic acid, beta.-amyrin, gamma sisterol, octodecanic acid, phytol, stigmasterol, vitamin E, lupeol were derived from SLF treated i.e. compared to control plants. Conclusion: The results of this study offer a platform of using SLF treated L.esculentum has an alternative source for various biological studies and it can be used as functional and pharmaceutical purposes.
Keywords: SLF, Lycopersicum esculentum, Phytochemical, GC-MS and FTIR
Biomedical Nanomaterials: A Short Insight of Applications
Authors: Singh Gurpreet , Bedi Preet Mohinder Singh , Faruk Abdul 
Published: January 2019

Nanotechnology is rapidly expanding area in the field of science that helps us to manipulate matter at the atomic and molecular level thus having varied and new properties with huge potential. It is the study of extremely small particles ranging from 1-1000 nm. This small size imparts the nanoscale devices their applications in medicine and physiology while permitting them to interact with cells and tissues at subcellular level, with bioreceptors on both the surface and inside of cell membrane thus providing a high degree of functional specificity to the systems. Nanodevices in medicine involves applications of nanoparticles, polymeric micelles, dendrimers, nanotubes, inorganic nanoparticles, nanocrystals and quantam dots which are currently under development as well as constitute the major part of longer range research. Improvement in drug solubility, high drug loading capacity, ease of surface modification, increase in blood circulation time, controlled release and highly site-specific targeted delivery are the advantages responsible for such wide applications of these nanomaterials. The applications include drug and gene delivery to specific site, delivery and detection of specific proteins, biological imaging and biosensors, DNA probing, tissue engineering, pathogens and tumor detection. This article has made an attempt to cover the few insights of nanotechnology as an interesting approach and illustrates various nanosystems along with their advantages, applications and well approved commercially available products.
Keywords: Polymeric micelles, Dendrimers, Nanotubes, Inorganic nanoparticles, Nanocrystals and Quantam dots
A General View of the Role of Specific nutrients in the Prevention of Cardiovascular Diseases
Authors: Maralla Swaroopa. , Bharathi D 
Published: January 2019

Nearly, 15.3 million people are estimated to die from cardiovascular diseases every year; and this represents one-third of all global deaths from all causes. Lifestyle factors, including nutrition, play an important role in the etiology of Cardiovascular Disease (CVD). A high intake of dietary fats strongly influences the risk of developing cardiovascular disease (CVD). Diet offers incredible opportunities for prevention of cardiovascular disease. Dietary patterns are useful for reducing CVD risk factors, and some have also shown a favourable effect on plaque regression and CVD mortality. Thus, every patient need to adopt a dietary approach that conforms to his or her personal preferences; however, implementing relevant and successful dietary changes is the greatest challenge for preventive cardiovascular medicine.
Keywords: Dietary Patterns, Cardiovascular Disease, Risk Factors, Mortality and Preventive Cardiovascular Medicine.
Oral dissolving film: present and future aspects
Authors: Kaur Prabhjot , Garg Rajeev 
Published: January 2019

Over the past few decades, tendency toward innovative drug delivery systems has almightily increased attempts to ensure efficacy, safety and patient acceptability. As discovery and development of new chemical agents is a complex, expensive and time consuming process, so recent trends are shifting toward designing and developing innovative drug delivery systems for existing drugs. Orally fast dissolving film is the kind of drug delivery system which when placed in the oral cavity, disintegrate or dissolve within a few seconds without the intake of water. Oral fast dissolving film is relatively new dosage form in which thin film is prepared using hydrophilic polymers, which rapidly disintegrate or dissolves on tongue or in the buccal cavity It is an alternative platform for molecules that undergoes high first pass metabolism. The method of preparation for oral dissolving film. Solvent casting, Semisolid casting, Hot melt extrusion, Solid dispersion extrusion, Rolling The current evaluation gives an account of different formulations methods of preparation and quality control of the fast dissolving oral thin film.
Keyword: First pass metabolism, Tensile strength, Fast Dissolving Oral Film etc.