bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calcium-mediated amyloid co-aggregation of S100A1 and S100A8 proteins
Authors: Karalkevičiūtė Viktorija , Baronaitė Ieva , Peštenytė Aistė , Veiveris Dominykas , Usevičius Gediminas , Šimėnas Mantas , Žiaunys Mantas , Smirnovas Vytautas , Šulskis Darius 
Published: November 2024

AbstractThe S100 family consists of calcium binding proteins that are largely known for their contribution to the neuroinflammatory processes. They are associated with various cardiac and neurological functions as well as related diseases. A few S100 proteins can form unspecific or amyloid aggregates in neuropathologies and thus play a part in dementia pathogenesis. Among all S100 proteins, S100B and S100A9 aggregation properties are the most investigated, however, there is a lack of studies regarding other S100 members. In particular, S100A1 and S100A8 are also associated with neuropathies, but their interactions or aggregation are poorly understood. Therefore, in this study, we explored whether S100A1 and S100A8 proteins can form heterodimers, interact or co-aggregate.Our results revealed that S100A1 and S100A8 interactions and amyloid aggregation are driven by calcium ions. We observed that while S100A1 remains mostly stable, S100A8 forms various types of spherical or unspecific aggregates. While they do not form stable heterodimers like calprotectin, their transient interactions facilitate the formation of worm-like amyloid fibrils and the process is regulated by different calcium ion concentrations. At calcium ions saturation, both proteins are stabilized leading to inhibition of aggregation. Overall, by employing a diverse range of techniques from amyloid and protein-specific fluorescence detection to electron-electron double resonance spectroscopy, we elucidated interactions between S100 proteins that might otherwise be overlooked, enhancing our understanding of their aggregation behaviour.
Uncovering the electrical synapse proteome in retinal neurons viain vivoproximity labeling
Authors: Tetenborg Stephan , Shihabeddin Eyad , Kumar Elizebeth Olive Akansha Manoj , Sigulinsky Crystal L. , Dedek Karin , Lin Ya-Ping , Echeverry Fabio A. , Hoff Hannah , Pereda Alberto E. , Jones Bryan W. , Ribelayga Christophe P. , Ebnet Klaus , Matsuura Ken , O’Brien John 
Published: November 2024

AbstractElectrical synapses containing Connexin 36 (Cx36) represent the main means for communication in the mammalian nervous system. However, little is known about the protein complexes that constitute these synapses. In the present study, we applied different BioID strategies to screen the interactomes of Connexin 36 the major neuronal connexin and its zebrafish orthologue Cx35b in retinal neurons. Forin vivoproximity labeling in mice, we took advantage of the Cx36-EGFP strain and expressed a GFP-nanobody-TurboID fusion construct selectively in AII amacrine cells. Forin vivoBioID in zebrafish, we generated a transgenic line expressing a Cx35b-TurboID fusion under control of the Cx35b promoter. Both strategies allowed us to capture a plethora of molecules that were associated with electrical synapses and showed a high degree of evolutionary conservation in the proteomes of both species. Besides known interactors of Cx36 such as ZO-1 and ZO-2 we have identified more than 50 new proteins, such as scaffold proteins, adhesion molecules and regulators of the cytoskeleton. Moreover, we determined the subcellular localization of these proteins in AII amacrine and tested potential binding interactions with Cx36. Amongst these new interactors, we identified signal induced proliferation associated 1 like 3 (SIPA1L3), a protein that has been implicated in cell junction formation and cell polarity as a new scaffold of electrical synapses. Interestingly, SIPA1L3 was able to interact with ZO-1, ZO-2 and Cx36, suggesting a pivotal role in electrical synapse function. In summary, our study provides the first detailed view of the electrical synapse proteome in retinal neurons, which is likely to apply to electrical synapses elsewhere.
Quantitative mapping of pseudouridines in bacteria RNA
Authors: Sharma Shikha , Woodworth Brendan , Yang Bin , Duan Ning , Pheko Mannuku , Moutsopoulos Niki , Emiola Akintunde 
Published: November 2024

AbstractRNA pseudouridylation is one of the most prevalent post-transcriptional modifications, occurring universally across all organisms. Although pseudouridines have been extensively studied in bacterial tRNAs and rRNAs, their presence and role in bacterial mRNA remain poorly characterized. Here, we used a bisulfite-based sequencing approach to provide a comprehensive and quantitative measurement of bacteria pseudouridines. As a proof of concept inE.coli, we identified 1,954 high-confidence sites in 1,331 transcripts, covering almost 30% of the transcriptome. Furthermore, pseudouridine mapping enabled the detection of differentially expressed genes associated with stress response that were unidentified using conventional RNA-seq approach. We also demonstrate that in addition to pseudouridine profiling, our approach can facilitate the discovery of previously unidentified transcripts. As an example, we identified a small RNA transcribed from the antisense strand of tRNA-Tyr which represses expression of distal genes. Finally, we mapped pseudouridines in oral microbiome samples of human subjects, demonstrating the broad applicability of our approach in complex microbiomes. Altogether, our work highlights the advantages of mapping bacterial pseudouridines and provides a tool to study posttranscription regulation in microbial communities.
Coping with a protracted crisis: a case study of IT-Integrator in Ukraine
Authors: Kukuruza Oksana , Golovkina Nataliya , Omelchenko Nadiia 
Published: November 2024

Learning outcomes
This case study offers the following learning opportunities for students: to identify and assess how a management team can establish effective working relationships to build a cohesive team during times of severe crisis and to prepare business for the restoration period; and to find alternative ways aimed at restoring the company’s operations and suggest ways of adapting to the new, normal situation.

Case overview/synopsis
This case study examines the strategic decisions of IT-Integrator, a Ukrainian IT company, during the Russian invasion of Ukraine in 2022. It highlights the leadership of vice president Nadiia Omelchenko in navigating the crisis, focusing on initial chaos, the development of a business continuity plan and efforts to restore operations and ensure employee safety. Despite warnings, the outbreak of war on February 24, 2022, was unexpected, with no established emergency protocols. Companies independently decided on measures for safety and business continuity, especially those critical to infrastructure and banking. In 2021, IT-Integrator faced reluctance within its executive team regarding resource allocation for wartime scenarios. Omelchenko’s push for a comprehensive business continuity plan proved crucial. Despite the plan’s effectiveness, unpreparedness for the crisis’s scale hindered recovery efforts. During the early days of the invasion, Omelchenko managed the dual challenge of safeguarding the business and its employees amid uncertainty and workforce reduction. Each decision had significant implications, requiring a balance between immediate survival and future stability. The case of IT-Integrator underscores the importance of proactive crisis management, strategic planning and resilient leadership. Omelchenko’s experience offers valuable lessons for businesses facing similar crises, emphasizing preparedness, adaptability and a focus on both immediate and long-term recovery.

Complexity academic level
This case study is suitable for MBA and executive development programs.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 6: human resource management.
Regulatory fragmentation and management earnings forecasts
Authors: Xu Hongkang 
Published: November 2024

PurposeThis study examines the impact of regulatory fragmentation on the issuance and width of management earnings forecasts (MEFs) in US firms, exploring how the presence of multiple regulatory bodies influences corporate disclosure and transparency.Design/methodology/approachUsing data from the Federal Register from 2004 to 2019, the study employs a measure of regulatory fragmentation to analyze its effect on the likelihood and width of MEFs.FindingsThe research finds that heightened regulatory fragmentation is associated with an increased likelihood of firms issuing MEFs and a wider range of forecasted earnings, indicating a strategic use of MEFs to manage regulatory complexity.Originality/valueThe study offers a unique perspective by contrasting with literature that predominantly views regulatory fragmentation negatively, providing detailed insights into its complex effects on corporate communication. It suggests the strategic use of MEFs amid regulatory challenges, enhancing our understanding of how regulatory environments shape corporate communication strategies.
Ethical reasoning in technology: using computational approaches to integrate ethics into AI systems
Authors: Sholla Sahil , Reshi Iraq Ahmad 
Published: November 2024

Purpose
This paper does not concern with the “why” of ethics. Such questions are typically of interest to philosophers and are outside the scope of this work. In the next section, the authors offer a look into “what” of ethics, i.e. various types and subtypes of ethics. Subsequently, the authors explore “how” of ethics, by summarising various computational approaches to ethical reasoning offered by researchers in the field.

Design/methodology/approach
The approaches are classified based on the application domain, ethical theory, agent type and design paradigm adopted. Moreover, promising research directions towards ethical reasoning are also presented.

Findings
Since the field is essentially interdisciplinary in nature, collaborative research from such areas as neuroscience, psychology, artificial intelligence, law and social sciences is necessary. It is hoped that this paper offers much needed insight into computational approaches for ethical reasoning paving way for researchers to further engage with the question.

Originality/value
In this paper, the authors discussed vaious computational approaches proposed by researchers to implement ethics. Although none of the approaches adequately answer the question, it is necessary to engage with the research effort to make a substantial contribution to the emerging research area. Though some effort has been made in the design of logic-based systems, they are largely in stages of infancy and merit considerable research.
Impact of market classifications on the innovative use of financing and dividend techniques: a global review of survey papers
Authors: Addico Nene Lartey , Amewu Godfred , Owusu-Ansah Anthony , Daniels Edward 
Published: November 2024

Purpose
This study aims to investigate the belief that the innovative/skilled use of financing and dividend policy decision techniques depends on the firm’s host market classifications (frontier, emerging and developed markets).

Design/methodology/approach
Using survey studies with similar questions, the authors reviewed, tallied and compared firm characteristic subgroup mean values of 2.4 and above (≥ 60% usage rate) per technique per market classification for the financing decisions analysis. In the dividend policy analysis, the authors tabulated existing rank results per market classification.

Findings
Managers in Malaysia significantly issue stock based on whether the firm's recent profits have been sufficient to fund their activities; this technique is of low value to US and Ghanaian managers. Managers in Ghana significantly limit their debt so their customers/suppliers are not worried about the firm going out of business; this technique has low value for Malaysian and US managers. Managers in Malaysia significantly issue debt when it gives investors a better impression of their firm prospects than issuing stock; this technique is not valuable to Ghanaian and US managers. On dividends, Ghanaian and sanctioned Iranian managers significantly consider cash availability before a dividend decision; this has low value to emerging and developed market managers.

Practical implications
These findings suggest that managers must customise their sets of valuable financing and dividend techniques to reflect the business risks and hurdles per their firm’s host market classification – as markets may determine a technique’s usefulness. Also, the innovative/skilful use of financing and dividend techniques decreases as managers move from developed to frontier markets, possibly due to degrading market conditions.

Originality/value
A global comparative study of survey literature covering frontier, emerging and developed markets is rare in the literature.
Production system state analysis and improvement using VSM, simulation, and fuzzy logic
Authors: Noueihed Karim , Hamzeh Farook 
Published: November 2024

PurposeThe paper aims to provide a door-to-door holistic perspective on the state of a production system based on several metrics and their relationships, to utilize a combination of tools and techniques and to develop a unique model for production system assessment. The study addresses the limitations of narrow approaches by proposing a new metric as a single indicator of the production system’s overall state.Design/methodology/approachThe research methodology adopted is design science research. It proposes a framework that combines value stream mapping, simulation and fuzzy logic to study the impact of different lean interventions on the overall state. An offsite construction facility was used as a case study. Data were gathered using methods such as ethnography, interviews, time and motion studies, shopfloor observations, video surveillance and database exploration.FindingsThe paper highlights how some interventions can have local improvements but lead to negative impacts on the overall system state. It emphasizes the importance of having a holistic approach to analyze and improve the true state of a production system.Research limitations/implicationsThe study excludes impacts of the supply chain and assumes the system to be confined within the shopfloor. Researchers are encouraged to include those variables in future studies.Practical implicationsThe study presents a practical framework and tool that can be tailored to any production system and be used to improve its performance.Originality/valueThis paper proposes a unique framework and a new metric for system state assessment and improvement.
At the crossroads of inflation: analyzing Central Bank responses in noneconomic crises
Authors: Bajra Ujkan Q. , Gjeçi Ardit , Cadez Simon 
Published: November 2024

Purpose
This study aims to thoroughly investigate the complexity of inflation dynamics in the context of two significant global crises, the COVID-19 pandemic and the ongoing geopolitical tensions in Ukraine. The primary goal is to examine the effects of several factors, such as interest rates, currency indices and unemployment, on inflation during extended periods of noneconomic crises.

Design/methodology/approach
This research uses a comprehensive data set spanning 75 months across the USA and the Euro Area, using the Generalized Method of Moments two-step regression methodology for analysis. The study examines the relationships between interest rates, currency indices, unemployment and inflation during extended noneconomic (financial) crises. This rigorous approach offers a nuanced understanding of how these factors interact and influence inflation.

Findings
The study highlights the crucial role of interest rates in controlling inflation during crises. Specifically, an interest rate increase of over 1.75% negatively impacts inflation, with more substantial rate hikes having a faster effect. The analysis reveals a minimal correlation between currency devaluation and inflation, emphasizing the predominant influence of interest rates. In addition, a notable negative correlation between unemployment and inflation is observed, indicating that higher unemployment rates tend to coincide with lower inflation levels.

Practical implications
The study’s findings offer valuable insights for central banks and policymakers tasked with managing inflation in times of crisis. By underscoring the effectiveness of interest rate adjustments and the limited influence of currency depreciation, this research provides critical guidance for formulating effective monetary policy during economic challenges.

Originality/value
This study contributes to existing literature by providing a comprehensive analysis of inflation dynamics during noneconomic crises, using a robust database and using advanced econometric techniques. The findings provide new insights into the role of interest rates, currency indices and unemployment in shaping inflation dynamics in times of noneconomic crises. These findings enhance the understanding of monetary policy strategies in challenging economic environments.
Integration and implementation of LSS 4.0 practices: a perspective from manufacturing and service sectors
Authors: Citybabu G. , Yamini S. 
Published: November 2024

PurposeLean Six Sigma (LSS) is often perceived as a foundation for implementing Industry 4.0 (I4.0) within an organization, with the two strengthening each other in different ways. The purpose of this article is to examine the evolution, trends and motivation for merging LSS and I4.0 and to gain insights into LSS 4.0 in both manufacturing and service sectors. This article also explores the effective methods for integrating and implementing LSS 4.0 in both manufacturing and service sectors, along with conducting a Strengths, Weaknesses, Opportunities and Threats (SWOT) analysis of LSS 4.0.Design/methodology/approachBy conducting a systematic literature review in compliance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, relevant literature from 2010 to 2023 was reviewed using appropriate keywords and research criteria. The objective was to identify, select and evaluate research articles related to LSS 4.0. Consequently, 159 relevant papers were located in databases, including Elsevier, Taylor and Francis Group, IEEE, Springer, Emerald Insights, Scopus and non-Scopus databases. Additionally, this review aimed to understand the progress of LSS 4.0 from the manufacturing and service sector context and to perform a SWOT analysis of LSS 4.0.FindingsThis review reveals a significant surge in the literature on LSS and I4.0 over the past three years. The research articles were categorized based on various themes, including the publisher, journals, types of articles and geographical factors such as country and continent. Additionally, the review examined the progress of LSS 4.0 in both manufacturing and service sectors. A SWOT analysis was also performed to identify the SWOT of LSS 4.0.Originality/valueThis is the first exclusive research work to conduct a SWOT analysis of LSS 4.0. This research article is highly valuable for academicians, researchers, policymakers and practitioners as it helps to identify the SWOT of LSS 4.0. Furthermore, it provides an overview of the progress of LSS 4.0 from both manufacturing and service sector perspectives, and significant case studies are discussed.
Lacticaseibacillus rhamnosus LRa05 alleviates cyclophosphamide‐induced immunosuppression and intestinal microbiota disorder in mice
Authors: Zhu Mingming , Yang Lvzhu , Kong Sufen , Bai Yuyuan , Zhao Bin 
Published: November 2024

AbstractProbiotics play a crucial role in regulating the gut microbiota and enhancing immune response. Oral administration of probiotics modulates intestinal microbiota composition and immune homeostasis. In this study, we investigated the immunoregulatory effect of Lacticaseibacillus rhamnosus LRa05 on cyclophosphamide (CTX)‐induced immunosuppressive mice. The results showed that oral administration of LRa05 reduced weight loss, restored immune organ indices, and maintained the structural integrity of the intestinal tissue in CTX‐treated mice. Moreover, oral administration of LRa05 exhibited immune‐modulating properties by promoting the secretion of cytokines (tumor necrosis factor‐α, interleukin‐1β, interleukin‐10, and secretory immunoglobulin A) in serum. Moreover, the analysis of 16S rRNA amplicon sequencing revealed that LRa05 increased gut microbiota diversity and regulated its composition. In detail, LRa05 intervention restored the Firmicutes/Bacteroidota ratio and significantly increased the relative abundance of Lachnospiraceae_NK4A136_group, Oscillibacter, Alloprevotella, Parasutterella, and Roseburia in immunocompromised mice. Conversely, the abundances of Helicobacter, Bacteroides, and unclassified_Desulfovibrionaceae were significantly decreased after administration of LRa05. Based on these findings, orally administered LRa05 could effectively maintain intestinal microbiota homeostasis and regulate immunity, suggesting the potential of L. rhamnosus LRa05 as a candidate probiotic strain in the application of dietary supplement.Practical ApplicationSupplement with L. rhamnosus LRa05 can improve immunity, regulate gut microbiota and promote body health.
Visual perceptual artefacts in cardiothoracic imaging: Implications for diagnostic accuracy and strategies for mitigation
Authors: Stirrat Thomas P , Chang Michael , Desai Anushka , Thiru Shankar , Chen Daniel T , Waller Joseph , Kamona Aws , Umair Muhammad 
Published: November 2024

SummaryVisual perceptual artefacts are distortions or illusions in medical image interpretation arising from the human visual system rather than hardware or imaging acquisition processes. These artefacts, emerging at various visual processing stages, such as the retina, visual pathways, visual cortex, and cognitive interpretation stages, impact the interpretation of cardiothoracic images. This review discusses artefacts including Mach bands, Dark Rim, Background Effects, Ambiguous Figures, Subjective Contours, and the Parallax Effect. A thorough literature search was conducted using PubMed and Google Scholar. Search terms included ‘visual perceptual artifacts’, ‘cardiothoracic imaging’, ‘Mach bands’, ‘dark rim artifact’, ‘stress cardiac MRI’, and ‘radiology visual illusions’. Inclusion criteria encompassed studies focusing on visual perceptual artefacts in cardiothoracic imaging published in peer‐reviewed journals. Studies not addressing visual perceptual artefacts or those focusing on imaging technology, hardware, or software‐related artefacts were excluded. Duplicate studies were removed, and a final selection of 32 studies was analysed. Several key visual perceptual artefacts relevant to cardiothoracic imaging were identified. Mach bands, caused by lateral inhibition in the retina, create false contrasts at object boundaries, potentially mimicking pathologies such as fractures or pneumothoraces. The dark rim artefact observed on stress cardiac MRI, resulting from Gibbs ringing or truncation artefacts, can simulate perfusion defects and complicate diagnosis. Lateral inhibition can possibly accentuate the appearance of the dark rim artefact. Artefacts also fall under illusions of sensation, perception, and image formation. These illusions present both risks and benefits to diagnostic radiology. Recognizing visual perceptual artefacts is crucial for improving diagnostic accuracy and developing strategies to mitigate their impact. A comprehensive understanding and training on these artefacts are essential for radiologists to enhance interpretive skills, reduce diagnostic errors, and ultimately improve patient care in radiology.