bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bergamottin exerts anticancer effects on human colon cancer cells via induction of apoptosis, G2/M cell cycle arrest and deactivation of the Ras/Raf/ERK signalling pathway
Authors: Zhang Hao , Yang ShuTao , Lin Tao 
Published: August 2019

IntroductionAccumulating evidence has shown the potential of bergamottin as an anticancer agent. The present study was undertaken to evaluate the anticancer affects of bergamottin (µM) against colon cancer cells.Material and methodsAntiproliferative effects were evaluated by WST-1 cell viability assay. Apoptotic effects were studied by DAPI and Annexin V/PI staining. Cell cycle analysis was carried out by flow cytometry. Transwell assay was used to study the effects on cell invasion. Protein expression was estimated by the western blot method.ResultsThe results showed that bergamottin suppresses the proliferation of all the human colon cancer cell lines. Nonetheless, the growth inhibitory effects of bergamottin on the HT-29 and RKO cells were more significant (IC50, 12.5 µM). The anticancer effects of bergamottin on the HT-29 and RKO cells were mainly due to apoptosis. Bergamottin could considerably increase the expression of Bax and reduce the expression Bcl-2. The cleavage of caspase-3, 8 and 9 was also enhanced upon bergamottin treatment of the colon cancer cells. Flow cytometric analysis showed that bergamottin also induced G2/M cell cycle arrest of the HT-29 and RKO cells. Additionally, bergamottin could also suppress the invasion of HT-29 and RKO cells. The Raf/MEK/ERK pathway is regarded as one of the essential pathways involved in the development and progression of cancers. Herein, it was observed that bergamottin could concentration dependently block this pathway in colon cancer cells. In vivo study revealed that bergamottin could also suppress the growth of tumours in xenografted mice models.ConclusionsTaken together, bergamottin suppresses the proliferation of colon cancer cells and may be utilised in the development of chemotherapy for colon cancer.
The effect of the two-stage laparoscopic Fowler-Stevens operation on testicular growth and risk of atrophy in boys with intra-abdominal testes
Authors: Niedzielski Jerzy , Balinska Kinga , Wilk Damian , Slowikowska-Hilczer Jolanta 
Published: August 2019

IntroductionThe aim of our study was to evaluate testicular growth and risk of atrophy at different lengths of time from the 2SLF-SO in boys with intra-abdominal cryptorchidism.Material and methodsTwenty-seven boys aged one to 24 months were treated for 35 non-palpable testes. Twenty-one boys with 29 IAT underwent a two-stage laparoscopic Fowler-Stephens operation. The patients underwent ultrasound examination of the TV at six time points: before treatment (TP1), 3 months (TP2) and 9-12 months after laparoscopic F-S operation (TP3), 3 months after inguinal orchidopexy (TP4), 3–6 years of age (TP5) and 7–9 years of age (TP6). The testicular atrophy index (TAI) of the affected testicle was calculated.ResultsThe overall success rate of treatment was 93.1% (27/29). The median volume of the affected testicles before treatment was less than the median TV of the healthy gonad, but increased systematically at the successive time points, showing significant growth between the second and sixth time points (p < 0.02, R = 2.75). The median TAI value decreased at the subsequent time points from the level of 26% at TP1 to 5.4% at TP6, with no significant difference.ConclusionsTwo-stage laparoscopic Fowler-Stevens operation proved to be an effective procedure for the treatment of intra-abdominal testicles. The undescended testis had a chance to grow and to equalize with the healthy testis after this procedure. The incidence of testicular atrophy was low.
Knockdown of microRNA-130b improves doxorubicin sensitivity in bladder urothelial carcinoma by negatively regulating cylindromatosis expression
Authors: Li Bo , Zhang Hui 
Published: August 2019

IntroductionChemotherapeutic resistance reduces the sensitivity of bladder urothelial carcinoma (BUC) to chemotherapeutic drugs and contributes a barrier leading to treatment failure. The purpose of this research project is to investigate the regulatory effects of miR-130b on chemotherapeutic drug resistance of BUC and its mechanism.Material and methodsThe relative expression of miRNA-130b and cylindromatosis (CYLD) was examined using real-time quantitative PCR. The cell proliferation and doxorubicin sensitivity were detected with the enhanced CCK-8 assay. The specific combination of miR-130b and CYLD was verified with the luciferase reporter gene assay. Protein expression was detected by Western blot.ResultsOur study found that miR-130b was up-regulated in doxorubicin-insensitive BUC tissues and cell lines, and its high expression was negatively related to doxorubicin sensitivity in BUC. The miR-130b knockdown reduced the IC50 of doxorubicin and improved doxorubicin sensitivity of J82/Dox and T24/Dox cells. For the regulation mechanism analysis of miR-130b, bioinformatics analysis software was used to predict the potential targets of miR-130b, including the CYLD gene. The following luciferase activities assay, quantitative real time-PCR and western blot identified the CYLD gene as a target of miR-130b. Knockdown of CYLD reversed miR-130b’s regulatory roles in doxorubicin sensitivity in J82/Dox and T24/Dox cells.ConclusionsHigh expression of miR-130b is negatively related to doxorubicin sensitivity in BUC, and knockdown of miR-130b improves doxorubicin sensitivity in BUC by negatively regulating CYLD expression. Our findings will provide guidance for the clinical chemotherapy of BUC.
Induction chemotherapy plus nimotuzumab followed
by concurrent chemoradiotherapy for advanced
nasopharyngeal carcinoma
Authors: Song Xinmao , Wang Shengzi , Li Ji , Yan Li , Chen Fu , Wang Jie 
Published: August 2019

IntroductionThis study investigated the best mode for the application of nimotuzumab (Nimo) in combination with chemoradiotherapy to treat nasopharyngeal carcinoma (NPC).Material and methodsData were prospectively collected from 168 patients with NPC from September 2009 to February 2014. One hundred twelve patients received 2–3 cycles of induction chemotherapy (IC) followed by concurrent chemoradiotherapy (CCRT), and 56 patients with well-matched propensity scores received IC + CCRT + Nimo. Patients were divided into 3 subgroups according to the application schedule of Nimo: group A, IC + CCRT; group B: IC (combined with Nimo) + CCRT; and group C: IC + CCRT (combined with Nimo). The 5-year overall survival (OS) and progression-free survival (PFS) and adverse events were investigated.ResultsWith a median follow-up of 61.4 months (range: 1.7–96.5 months), the 5-year OS and PFS for group A vs. groups B + C were 74.8 ±4.1% versus 87.0 ±4.6% (p = 0.043) and 72.7 ±4.3% vs. 83.1 ± 5.1% (p = 0.243), respectively. The 5-year OS of group B was significantly improved over that of group A (93.0 ±4.8% vs. 74.8 ±4.1%, p = 0.038); however, there was no benefit to the 5-year PFS (89.3 ±5.9% vs. 72.7 ±4.3%, p = 0.144). The 5-year OS and PFS for group C were 80.4 ±7.9% and 76.4 ±8.5%, respectively, and there was no statistically significant difference from group A (p = 0.257 and p = 0.611, respectively). No significant increase in toxicities was observed with the addition of Nimo.ConclusionsNimo administered with chemoradiotherapy is effective for NPC. Nimo concurrent with IC followed by CCRT could be the optimal mode of sequential treatment.
Serum microRNA profiles in patients with autosomal dominant polycystic kidney disease show systematic dysregulation partially reversible by hemodialysis
Authors: Małachowska Beata , Tkaczyk Marcin , Chrul Sławomir , Zwiech Rafał , Młynarski Wojciech , Fendler Wojciech 
Published: August 2019

IntroductionThe impact of autosomal dominant polycystic kidney disease (ADPKD) on serum microRNAs (miRNA) is unknown.Material and methodsFor profiling experiment we recruited 30 patients from three equinumerous groups: controls, ADPKD and ADPKD on hemodialysis. From the last group extra samples were collected for in pre /post dialysis analysis. Additionally, 23 healthy volunteers were used for selected biomarker verification. Real-time PCR arrays were used for quantification of 752 miRNAs. Validation of selected miRNAs was performed in total RNA extracted from the serum and the exosomal fraction in pre /post dialysis samples.ResultsIn total, 37 significant circulating miRNAs were found to differ between ADPKD patients and controls. In validation, 3 miRNAs with the highest fold change in comparison of dialyzed vs non-dialyzed patients (miR-532-3p, miR-320b, miR-144-5p) were not significantly altered by hemodialysis and from the top down-regulated ones, miR-27a-3p was significantly lower after dialysis in both total and exosomal fractions, miR-20a-5p was down-regulated in the exosomal fraction and miR-16-5p was unaltered by hemodialysis. MiR-16-5p was selected as the best circulating biomarker of ADPKD. Circulating representatives of the miR-17 family sharing the same seed region (miR-20a-5p, miR-93-5p and miR-106a-5p) showed significantly lower expression among dialyzed vs. non-dialyzed patients and their exosomal fraction dropped after hemodialysis.ConclusionsThe serum miRNAs among ADPKD patients differ substantially depending on the stage of CKD. The exosomal fraction of miRNA was more affected by dialysis than the total one. There was a common pattern of down-regulation for circulating miR-17 family members sharing the same seed region.
Long non-coding RNA LINC01296 promotes progression of oral squamous cell carcinoma through activating the MAPK/ERK signaling pathway via the miR-485-5p/PAK4 axis
Authors: Zhang Shuangyue , Wang Xiaowei , Wang Dazhao 
Published: August 2019

IntroductionLong intergenic non-protein-coding RNA 1296 (LINC01296), a newly identified lncRNA, can function as an oncogenic driver to promote the development of multiple carcinomas. However, the effect of LINC01296 on oral squamous cell carcinoma (OSCC) is still unclear.Material and methodsWe determined the expression and role of LINC01296 in OSCC tissues and cell lines. The cell viability, migration and invasion were determined by MTT, wound healing assay and transwell assay, respectively. Flow cytometry was used for detecting cell cycle and apoptosis. The interaction and association between LINC01296, microRNA-485-5p (miR-485-5p) and p21 (RAC1) activated kinase 4 (PAK4) were analyzed by RNA immunoprecipitation (RIP) and luciferase reporter assays. The xenograft mouse model was established to detect the effect of LINC01296 on OSCC tumor growth.ResultsOur study showed that LINC01296 was over-expressed in OSCC tissues and cell lines. The level of LINC01296 was positively correlated with the patient’s tumor node metastasis (TNM) stage and nodal invasion. Knockdown of LINC01296 effectively inhibits cell viability, migration and invasion but promotes cell apoptosis in vitro. The in vivo experiment showed that LINC01296 knockdown inhibited OSCC tumor growth. The following analysis indicated that LINC01296 acted as a ceRNA for miR-485-5p, and PAK4 was identified as a direct target of miR-485-5p. Furthermore, we found that the effects of LINC01296 on OSCC progression were through regulating the expression of PAK4/p-MEK/p-ERK via sponging miR-485-5p.ConclusionsLINC01296 promote the cell cycle, proliferation, migration and invasion, and inhibit apoptosis of OSCC cells through activating the MAPK/ERK signaling pathway via sponging miR-485-5p to regulate PAK4 expression. These results suggested that the LINC01296/miR-485-5p/PAK4 axis was closely associated with OSCC progression. Our study provides a new insight into the molecular pathogenesis of OSCC, and may supply novel biomarkers for diagnosis and therapy of OSCC.
Hsa_circ_0017728 as an oncogene in gastric cancer by sponging miR-149 and modulating the IL-6/STAT3 pathway
Authors: Yang Chun , Deng Shaoping 
Published: August 2019

IntroductionCircular RNAs (circRNAs) have been identified as competing endogenous RNAs (ceRNAs) to mediate gene expression participating in the progression of multiple cancers, including gastric carcinoma (GC). However, the underlying molecular mechanisms by which circRNAs-modulated cell proliferation and apoptosis in GC had not been completely clarified. In our study, hsa_circ_0017728 as a potential oncogene competing endogenous RNA (ceRNA) was investigated in the progression and development of gastric carcinogenesis.Material and methodsHigh-throughput sequencing was used to determine differentially expressed circRNAs in GC tissues and corresponding non-cancerous tissues. The CCK-8 assay and Annexin V-fluorescein isothiocyanate/polyimide (Annexin V-FITC/PI) staining were performed to detect the cell viability and apoptosis in GC cells. In addition, gene expression and protein levels in GC tissues and cell lines were measured using RT-qPCR and western blotting, respectively.ResultsOur results demonstrated that the hsa_circ_0017728 expression level was up-regulated in GC tissues and cell lines and closely associated with poor overall survival and pathological differentiation, higher TNM stage and lymph node metastasis. Knockdown of hsa_circ_0017728 had the ability to cause inhibition of cell proliferation and migration and elevate the cell apoptosis rate in GC cells. We also discovered that hsa_circ_0017728 might serve as a ceRNA to sponge miR-149 and indirectly regulated the IL-6/STAT3 signaling pathway in GC cell proliferation and apoptosis.ConclusionsThe regulatory network of hsa_circ_0017728/miR-149/IL-6/STAT3 cascade signaling might provide a better understanding of gastric carcinogenesis and progression.
Motivation of cancer patients to help others and the relation between posttraumatic growth and helping
Authors: Trzmielewska Weronika , Zięba Mariusz , Boczkowska Marta , Rak Tomasz , Wrześniowski Szymon 
Published: August 2019

BackgroundPosttraumatic growth (PTG) is related to positive psychological changes following people’s struggle with trauma. The PTG model suggests that one of its possible consequences may be higher motivation towards helping behaviours. The aim of the research was to explore the influence of cancer illness on the motivation to help others, as perceived by participants, and the mechanisms behind such behaviour.Participants and procedureQuantitative and qualitative methods were used to explore the main motives to help others among cancer patients. The study also investigated the relation of perceived growth (PTG) and the frequency of providing help. Female cancer patients (n = 100) com-pleted a Polish version of the Posttraumatic Growth Inventory and a questionnaire measuring frequency of providing help to-wards ill and healthy individuals. Some of the subjects (n = 29) were also interviewed to assess the main perceived motives to help others.ResultsPositive rho Spearman correlations between PTG and the frequency of help given towards ill individuals and healthy ones were found. Based on thematic analysis four motives of helping were identified: empathy, self-enhancement, internalized standards of behaviour, searching for the sense of life.ConclusionsThis findings highlight the positive association between cancer patients’ involvement in helping other cancer patients and posi-tive life changes after experience of cancer.
Footedness in fitness and technical skills in young soccer players
Authors: Lipecki Krzysztof 
Published: August 2019

PurposeThe aim of the study was to identify differences in fitness abilities and technical skills between young right-footed
and left-footed soccer players.MethodsThe measurements included data collected from 111 male footballers aged 12–17 years (divided into 3 age
categories: U13, U15, U17). Fitness abilities (5-m, 10-m, 30-m sprint, envelope run, standing long jump) and technical skill
(dribbling, juggling, short passes, long passes) were measured.ResultsThe results failed to show significant differences (<i>p</i> > 0.05) in speed, agility, or explosive strength of lower limbs
between right- and left-footed soccer players in the U13, U15, and U17 categories. No significant differences between soccer
players (right-footed, left-footed) were found in the dominant leg performance (<i>p</i> > 0.005), whereas for the non-dominant leg,
left-footed U15 players had higher performance in juggling (<i>p</i> < 0.05) and long passes (<i>p</i> < 0.01). Regardless of age category,
left-footed players were characterized by a lower range of asymmetry in all technical skills. Significant differences were
observed only for dribbling skills in U13 (<i>p</i> < 0.05).ConclusionsThe type of lateral dominance (right-footedness, left-footedness) does not differentiate between young soccer
players in terms of fitness abilities. Left-footed footballers are more symmetrical in technical preparation compared with
the right-footed ones. With age and experience in training, a reduction in functional asymmetry in technical skills is
observed between lower limbs in both right- and left-footed soccer players. Regardless of the type of lateral dominance,
young players have their reserves in terms of symmetrical technical preparation.
Are there any differences in the agility performance tests among goalkeepers depending on the type of surface? A crossover study
Authors: Oliveira Leonardo Dos Santos , Holanda Vinícius Serrano Braz de , Brito-Gomes Jorge Luiz de , Pontes Nayara Elis Cabral , Souza Geovani Garcia de , Aniceto Rodrigo Ramalho , Pérez-Gómez Jorge 
Published: August 2019

PurposeThe aims of the study were: (a) to compare agility in the T-test (TT) and square test (ST) on different surfaces (natural grass vs. wood); (b) to compare agility performance in soccer and futsal young male goalkeepers.MethodsIn a crossover study, 8 soccer (age: 16 ± 2 years, body mass: 68.5 ± 10.5 kg, height: 1.69 ± 0.4 m, relative body fat mass: 22.4 ± 6.5%) and 8 futsal (age: 16 ± 1 years, body mass: 67.7 ± 8.4 kg, height: 1.70 ± 0.4 m, relative body fat mass: 21.0 ± 6.1%) goalkeepers randomly performed TT and ST on both surfaces. All comparisons were adjusted for body mass and relative body fat mass.ResultsSurface had a slight effect (0.066 d 0.163) on agility in both tests irrespective of the sport (TT<sub>grass</sub>: 10.90 ± 1.04 s, TT<sub>wood</sub>: 10.80 ± 0.96 s, ST<sub>grass</sub>: 5.82 ± 0.32 s, ST<sub>wood</sub>: 5.87 ± 0.36 s, p > 0.05). No differences were found in agility performance between soccer (TT<sub>grass</sub>: 11.10 ± 0.23 s, TT<sub>wood</sub>: 11.10 ± 0.28 s, ST<sub>grass</sub>: 5.75 ± 0.08 s, ST<sub>wood</sub>: 5.85 ± 0.14 s) and futsal (TT<sub>grass</sub>: 10.60 ± 1.07 s, TT<sub>wood</sub>: 10.50 ± 0.90 s, ST<sub>grass</sub>: 5.81 ± 0.31 s, ST<sub>wood</sub>: 5.84 ± 0.31 s) goalkeepers, regardless of the test or surface (<i>p</i> > 0.05). There were strong relationships among the conditions (0.69 ≤ <i>r</i> ≤ 0.96). Moreover, the tests applied on different surfaces systematically produced similar results, and interchange of test surfaces was supported by parallel reliability.ConclusionsSurface effect for both tests does not seem enough to induce statistical differences in the agility of soccer and futsal young male goalkeepers. Goalkeepers’ agility performances were similar in both sports.
The Impact of Using the First and Third Parts of the Cort Program on the Development of Critical Thinking for Talented Students in Tafila Governorate
Authors: Rabbo Dr. Jehad .Abed 
Published: August 2019

This study aims to examine the impact of using some of the skills of the (CoRT) program on the development of critical thinking skills among a sample of talented students in King Abdullah II School of Excellence in Tafila Governorate. The sample consisted of 60 male and female students randomly selected. They were divided into two groups: an experimental group that consisted of 30 students exposed to the acquisition of the skills of the CoRT program and the control group that consisted of 30 students who did not study the program. The Watson Glaser critical thinking test was also applied. The results shows a statistically significant effect, at (α≤ 0.05), in improving the critical thinking skills of the students of the experimental group and shows a statistically significant effect on the test of critical thinking skills due to the variable of educational level and for the favor of the ninth grade. The study also reveals that there were no statistically significant differences in the test of critical thinking skills in relation to the the gender variable. The researcher recommends developing the teacher competencies to teach the skills of thinking in general and critical thinking skills, in particular, through the conducting of courses, seminars, and workshops in the CoRT program.
Floor diaphragms and a truss method for their analysis
Authors: Scarry J.M. 
Published: August 2019

Floor diaphragms form a critical component of seismic resistant buildings, but unfortunately, in the main their analysis and design in New Zealand leaves much to be desired. No worse example exists than the CTV Building in Christchurch. Despite the critical importance of diaphragms, there is a paucity of code provisions and design guidance relating to them.
Using generic examples, the author describes a number of common diaphragm design deficiencies. These include diaphragms where valid load paths do not exist; diaphragms where the floors are not properly connected to the lateral load resisting elements, diaphragms that lack adequate flexural capacity and where re-entrant corners are not properly accounted for, and transfer diaphragms into which the reactions from the walls above cannot be properly introduced or transmitted.
Three main types of seismic diaphragm action are discussed – ‘inertial,’ ‘transfer’ and ‘compatibility.’ These are, respectively, the direct inertial load on a floor that must be carried back to the lateral load resisting elements, the transfer forces that occur when major changes in floor area and lateral load resisting structure occur between storeys, and the compatibility forces that must exist to force compatible displacements between incompatible elements, such as shear walls or braced frames and moment frames, or as a result of redistribution.
The author presents a simple Truss Method that allows complex diaphragms to be analysed for multiple load cases, providing accurate force distributions without the multiple models that conventional strut and tie methods would require. Being a type of strut and tie method, the Truss Method is compliant with requirements in NZS3101:2006 [1] to use strut and tie models for the analysis and design of certain aspects of diaphragm behaviour.
Adsorção de óleos lubrificantes em casca de arroz
Authors: Hoss Louise , Loebens Larissa , Neto Luiz Alfredo Bohlke , Quadro Maurizio Silveira , Andreazza Robson , Gadotti Gizele Ingrid 
Published: August 2019

O uso de óleos lubrificantes, apesar de ter o benefício de aumentar a eficiência de equipamentos e sua vida útil, causa prejuízos ambientais pela geração de resíduos contaminados e poluição de corpos hídricos. O tratamento destes resíduos é fundamental para garantir a sanidade do meio ambiente. Em vista disso, uma solução de baixo custo é o aproveitamento de resíduos, como a casca de arroz, para utilização como meio adsorvente. A casca de arroz foi utilizada sem pré-tratamento em tempos de reação de cinco, quinze e trinta minutos, uma, duas e quatro horas. Os experimentos foram feitos em bateladas, com relações de adsorbato/adsorvente de 5,0 mL.g-1 e de 2,0 mL.g-1. Com base nos resultados foram estabelecidas as curvas de adsorção do sistema. Foi observado que a adsorção do óleo na casca de arroz ocorre de forma rápida, sendo adsorvidos entre 65 e 70% da capacidade total do adsorvente nos primeiros cinco minutos de contato. O equilíbrio dos processos de adsorção/dessorção mostrou que a capacidade máxima de retenção de óleo na casca de arroz foi de 1,91 g.g-1 (2,20 mL.g-1). A remoção de óleo na proporção de 2,0 mL.g-1 foi de até 94%, reduzindo a concentração de óleo no efluente de 86,7 g.L-1 para 5,4 g.L-1.