bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic analysis of the effects of cell culture density on the metastasis of breast cancer cells
Authors: Guo Man‐Lan , Sun Mi‐Xin , Lan Jin‐Zhi , Yan Li‐Sha , Zhang Jing‐Juan , Hu Xiao‐Xia , Xu Shu , Mao Da‐Hua , Yang Hai‐Song , Liu Ya‐Wei , Chen Teng‐Xiang 
Published: February 2019

Cancer cell progression and proliferation increase cell density, resulting in changes to the tumour site, including the microenvironment. What is not known is if increased cell density influences the aggressiveness of cancer cells, especially their proliferation, migration, and invasion capabilities. In this study, we found that dense cell culture enhances the aggressiveness of the metastatic cancer cell lines, 4T1 and ZR‐75‐30, by increasing their proliferation, migration, and invasion capabilities. However, a less metastatic cell line, MCF‐7, did not show an increase in aggressiveness, following dense cell culture conditions. We conducted a differential proteomic analysis on 4T1 cells cultured under dense or sparse conditions and identified an increase in expression for proteins involved in migration, including focal adhesion, cytoskeletal reorganization, and transendothelial migration. In contrast, 4T1 cells grown under sparse conditions had higher expression levels for proteins involved in metabolism, including lipid and phospholipid binding, lipid and cholesterol transporter activity, and protein binding. These results suggest that the high‐density tumour microenvironment can cause a change in cellular behaviour, leading towards more aggressive cancers.Significance of the studyMetastasis of cancer cells is an obstacle to the clinical treatment of cancer. We found that dense cultures made metastatic cancer cells more potent in terms of proliferation, migration, and invasion. The proteomic and bioinformatic analyses provided some valuable clues for further intensive studies about the effects of cell density on cancer cell aggressiveness, which were associated with events such as pre‐mRNA splicing and RNA transport, focal adhesion and cytoskeleton reorganization, ribosome biogenesis, and transendothelial migration, or associated with proteins, such as JAM‐1 and S100A11. This investigation gives us new perspectives to investigate the metastasis mechanisms related to the microenvironment of tumour sites.
Long‐term follow‐up of covered stent implantation for various coronary artery diseases
Authors: Hachinohe Daisuke , Latib Azeem , Laricchia Alessandra , Iannopollo Gianmarco , Demir Ozan M. , Ancona Marco B. , Mangieri Antonio , Regazzoli Damiano , Giannini Francesco , Azzalini Lorenzo , Mitomo Satoru , Chieffo Alaide , Montorfano Matteo , Carlino Mauro , Colombo Antonio 
Published: February 2019

AbstractObjectivesThe aims of this study were to evaluate the long‐term clinical outcomes after coronary covered stent (CS) implantation and to compare the results according to the indications.BackgroundTo date, data on the long‐term follow‐up of coronary CS are limited, and no studies have been conducted to compare outcomes on the basis of specific lesions treated.MethodsA total of 190 consecutive patients (212 lesions) implanted with CS and surviving until discharge between May 1997 and February 2017 were enrolled in this retrospective study. Clinical outcomes were evaluated and compared using the Gehan–Breslow–Wilcoxon test.ResultsCS was mainly used for the treatment of a saphenous vein graft (SVG) (51.4%), followed by coronary artery perforation (CAP) (25.0%) and coronary artery aneurysm (CAA) (11.8%). The median follow‐up duration was 6.0 (interquartile range: 1.6–13.5) years. Target vessel myocardial infarction (MI), target vessel occlusion, target lesion revascularization, and stent thrombosis, frequently occurred in the early follow‐up period (7.3%, 16.6%, 21.5%, and 8.9% at 1 year, respectively) and continued to increase throughout the long‐term follow‐up (14.8%, 38.6%, 38.7%, and 17.8% at 10 years, respectively). Target vessel occlusion, target vessel MI, and ST were not identified in CAA with superiority to CAP (Gehan–Breslow–Wilcoxon p‐value = 0.010, 0.047, and 0.046, respectively). SVG had a higher rate of target vessel occlusion than CAA (p = 0.004).ConclusionsClinical events after CS implantation frequently occurred in the early period and steadily increased in the long‐term period. CS implantation in CAA was associated with fewer adverse cardiac events and may lead to safe and acceptable outcomes. However, CS implanted in CAP and SVG are associated with a high risk of long‐term cardiac events.
Sirolimus‐eluting BiOSS LIM dedicated bifurcation stent in the treatment of unprotected distal left main stenosis
Authors: Briguori Carlo , Visconti Gabriella , Golino Marco , Focaccio Amelia , Signoriello Giuseppe 
Published: February 2019

AbstractBackgroundProximal optimization technique (POT) has been proposed to adapt the conventional drug‐eluting stent (DES) with the fractal anatomy of the bifurcation. However, only few DES are labeled for post‐expansion beyond 5.0 mm. Furthermore, recrossing in the side branch (SB) through the main vessel (MV) stent cells may be challenging.ObjectivesTo compare the sirolimus‐eluting, balloon‐expandable dedicated bifurcation stent BiOSS LIM DES versus the second generation DES in the treatment of distal unprotected left main coronary arteries (ULMCAs) lesions.MethodsForty‐two consecutive patients with distal ULMCA lesions were treated with the BiOSS LIM (BiOSS LIM group) in our center. A matched‐group of patients treated with second‐generation DES was selected from our database (Control group). The primary endpoint was the procedural complication rate, including (a) SB occlusion, defined as intraprocedural TIMI flow grade <3 immediately after MV stenting; and/or (b) trouble in SB access, defined as the need of ≥2 guidewires or a failure to recross in the SB trough the MV stent cells. The need of POT in the two groups was also analyzed.ResultsThe primary endpoint occurred in four (9.5%) patients in the BiOSS LIM group and in 13 (31%) in the Control group (p = 0.028; OR = 4.25; 95% confidence interval: 1.25–14.43). POT was performed more often in the Control group (71% vs. 35%; p = 0.004).ConclusionsCompared to conventional DES, the BiOSS LIM stent (1) facilitates SB recrossing and (2) fits well with the fractal anatomy of the left main bifurcation.
The predictors of complications in balloon pulmonary angioplasty for chronic thromboembolic pulmonary hypertension
Authors: Ikeda Nobutaka , Kubota Shuji , Okazaki Toru , Iijima Raisuke , Hara Hidehiko , Hiroi Yukio , Nakamura Masato 
Published: February 2019

AbstractObjectivesThe aims of this study were to (1) evaluate risk factors of complications of balloon pulmonary angioplasty (BPA) and (2) assess the mechanism of the complications.BackgroundBPA represents a promising treatment option in patients with inoperable chronic thromboembolic pulmonary hypertension (CTEPH). However, the complication ratio differs across reports, and the causes remain controversial.MethodsAll complications found by angiography and non‐contrasted computerized tomography (CT) immediately after BPA were recorded. New emergences of a ground‐glass pattern or consolidation in CT after BPA that were not recognized on CT images before BPA were counted as pulmonary bleeding. Lesion morphologies were classified into four types (web, ring, abrupt narrowing and occlusive lesions) according to selective pulmonary angiography.ResultsThirty patients consented to analysis of the BPA‐related images, and 879 lesions (112 sessions) were evaluated. One hundred and twenty‐two (99.2%) of 123 complications were confirmed to be associated with BPA procedures at the local area. In the multivariate analysis, occlusive lesions were the sole independent predictor of procedure‐related complications (adjusted odds ratio 5.83, 95%CI [1.94–17.47], p = 0.002). Hemodynamic parameters were not predictors of complications. CT images after BPA presented the predictive value for the occurrence of hemoptysis.ConclusionsAlmost all complications were attributed to operators' procedures. Lesion morphology was the sole predictor of BPA‐related complications, while hemodynamic parameters were not associated with the frequency of complications. CT scan images after BPA were useful to identify bleeding complications and to predict hemoptysis.
Case report: Atrioventricular block after transcatheter atrial septal closure using the Figulla® Flex II ASD occluder
Authors: Sato Jun , Kato Atsuko , Takeda Sho , Nishikawa Hiroshi 
Published: February 2019

AbstractWe report a 7‐year‐old male patient who developed severe atrioventricular block after transcatheter closure of the atrial septal defect with an Occlutech Figulla® Flex II ASD occluder (FSO). He had a small aortic rim and the defect measuring 22.3 mm by balloon sizing. When a 24 mm FSO was deployed, he developed Wenckebach second‐degree heart block; however, it recovered to sinus rhythm. Hence, the device was implanted. The rhythm deteriorated to a fixed 2:1 heart block within 7 hr. He underwent surgical retrieval of the device and closure of the defect. Intraoperative findings demonstrated the right atrium disk compressing the triangle of Koch, resulting in a small hematoma. The rhythm recovered completely by 7 days after the surgery. Care must be taken when a relatively large device is deployed in a patient with small rims as even “soft and flexible” device like the FSO can injure the endocardium.
Lumacaftor/ivacaftor initiation in two liver transplantation patients under tacrolimus and antifungal azoles
Authors: Chouchane Ikrame , Stremler‐Lebel Nathalie , Reix Philippe 
Published: February 2019

Key Clinical MessageWe report the initiation of CFTR modulator lumacaftor/ivacaftor combination (LUM/IVA) in two adolescents with cystic fibrosis who were treated with antifungal azoles (AZO) and tacrolimus (TCS) for liver transplantation. Despite multiple drug‐drug interactions, maintaining therapeutic TCS levels was achievable. During the following year, LUM/IVA was well tolerated, providing clinical benefits.
ReGeneraTing Agents (RGTA®): A new option for healing and improving treatment outcomes for traumatic and burn injuries of the hand
Authors: Roohi Sharifah Ahmad , Barritault Denis 
Published: February 2019

Key Clinical MessageCACIPLIQ20® significantly improved the outcomes of severe burn injuries of the hand. Healing was accelerated, with little or no scarring, allowing for greater mobility over the joints and maintained suppleness. Functional recovery was achieved in all cases.
Facile Preparation of 1T/2H‐Mo(S1‐xSex)2 Nanoparticles for Boosting Hydrogen Evolution Reaction
Authors: Lin Zhiping , Lin Bo , Wang Zongpeng , Chen Shougang , Wang Chengwei , Dong Mengyao , Gao Qiang , Shao Qian , Ding Tao , Liu Hu , Wu Shide , Guo Zhanhu 
Published: February 2019

Abstract2D transition metal dichalcogenides with a formula MoX2 (X=S or Se) have been explored as promising earth‐abundant catalysts for hydrogen evolution reaction (HER), especially those with 1T phases. Theoretical calculations and experiments have indicated that optimal catalytic performance can be achieved by regulating the atomic ratio of S and Se in Mo(S1‐xSex)2. However, the preparation of 1T phase Mo(S1‐xSex)2 is challenging because of the differences of S and Se elements. Here, we propose a facile and effective hydrothermal method for the production of 1T/2H Mo(S1‐xSex)2, with which products with various x values are prepared. The as‐prepared Mo(S1‐xSex)2 samples give a low Tafel slope of 42.8 mV dec−1 (x=0.25), and an overpotential of 161 mV (x=0.5), which are state‐of‐the‐art among the tremendous MoX2 works. The excellent electrocatalytic performance is derived from the coexistence of S and Se atoms which reduces the reaction potential energy, and the presence of the 1T phase which improves the conductivity. Additionally, the ratio of 1T phase to 2H phase of Mo(S1‐xSex)2 is found controllable over reaction temperature.
Catalytic Wacker‐type Oxidations Using Visible Light Photoredox Catalysis
Authors: Ho Yee Ann , Paffenholz Eva , Kim Hyun Jin , Orgis Benjamin , Rueping Magnus , Fabry David C. 
Published: February 2019

AbstractA combined palladium/photoredox catalytic system for the efficient oxidation of terminal olefins to the corresponding methyl ketones is presented. The interplay of air, water, and light leads to a protocol in which the stoichiometric oxidants required for oxidative palladium catalysis are substituted with catalytic, single‐electron transfer processes. Detailed mechanistic investigations revealed the role of the key components, in situ generated species, and catalysts. A broad range of substrates was examined in homogeneous as well as heterogeneous photoredox protocols, delivering the desired products in good yields.
Cell type‐specific distribution of GABAA receptor subtypes in the mouse dorsal striatum
Authors: Boccalaro Ida Luisa , Cristiá‐Lara Leonardo , Schwerdel Cornelia , Fritschy Jean‐Marc , Rubi Lena 
Published: February 2019

AbstractThe striatum is the main input nucleus of the basal ganglia, mediating motor and cognitive functions. Striatal projection neurons are GABAergic medium spiny neurons (MSN), expressing either the dopamine receptor type 1 (D1‐R MSN) and forming the direct, movement‐promoting pathway, or dopamine receptor type 2 (D2‐R MSN), forming the indirect movement‐suppressing pathway. Locally, activity and synchronization of MSN are modulated by several subtypes of GABAergic and cholinergic interneurons. Overall, GABAergic circuits in the striatum remain poorly characterized, and little is known about the intrastriatal connectivity of interneurons and the distribution of GABAA receptor (GABAAR) subtypes, distinguished by their subunit composition, in striatal synapses. Here, by using immunofluorescence in mouse tissue, we investigated the distribution of GABAARs containing the α1, α2, or α3 subunit in perisomatic synapses of striatal MSN and interneurons, as well as the innervation pattern of D1R‐ and D2R‐MSN soma and axonal initial segment (AIS) by GABAergic and cholinergic interneurons. Our results show that perisomatic GABAergic synapses of D1R‐ and D2R‐MSN contain the GABAAR α1 and/or α2 subunits, but not the α3 subunit; D2R‐MSN have significantly more α1‐GABAARs on their soma than D1R‐MSN. Further, interneurons have few perisomatic synapses containing α2‐GABAARs, whereas α3‐GABAARs (along with the α1‐GABAARs) are abundant in perisomatic synapses of CCK+, NPY+/SOM+, and vAChT+ interneurons. Each MSN and interneuron population analyzed received a distinct pattern of GABAergic and cholinergic innervation, complementing this postsynaptic heterogeneity. In conclusion, intra‐striatal GABAergic circuits are distinguished by cell‐type specific innervation patterns, differential expression and postsynaptic targeting of GABAAR subtypes.
Versatile Molten Salt Synthesis of Manganite Perovskite Oxide Nanocrystals and Their Magnetic Properties
Authors: Gonell Francisco , Alem Naoual , Dunne Peter , Crochet Guillaume , Beaunier Patricia , Méthivier Christophe , Montero David , Laberty‐Robert Christel , Doudin Bernard , Portehault David 
Published: February 2019

AbstractManganite perovskite oxides exhibit a wide range of properties relevant for catalysis, energy conversion and spintronics. Such behaviors can be deeply modified at the nanoscale where confinement and surface effects may prevail. Interesting properties could then emerge from manganite perovskite nanocrystals, although their synthesis remains a challenge. The present study reports a versatile synthesis of manganite perovskite nanocrystals by using molten salts as high temperature liquids in the 600–750 °C range. Through X‐ray diffraction, X‐ray photoelectron spectroscopy, scanning and transmission electron microscopies, we show that many substitutions on the A perovskite site are within reach by applying the approach to La2/3Sr1/3MnO3, La2/3Ca1/3MnO3 and La1/3Pr1/3Ca1/3MnO3 cubic‐shape nanocrystals with diameter of 20, 22 and 38 nm, respectively. The magnetic properties of these nanocrystals are then investigated at 5 K, highlighting the coexistence of a ferromagnetic phase typical of the bulk, with disordered and non‐magnetic domains, which presumably arise from the nanoscale and surface effects.
Selective intracellular delivery of perfluorocarbon nanodroplets for cytotoxicity threshold reduction on ultrasound‐induced vaporization
Authors: Ishijima Ayumu , Yamaguchi Satoshi , Azuma Takashi , Kobayashi Etsuko , Shibasaki Yoshikazu , Nagamune Teruyuki , Sakuma Ichiro 
Published: February 2019

AbstractBackgroundPhase‐change nanodroplets (PCNDs), which are liquid perfluorocarbon nanoparticles, have garnered much attention as ultrasound‐responsive nanomedicines. The vaporization phenomenon has been employed to treat tumors mechanically. However, the ultrasound pressure applied to induce vaporization must be low to avoid damage to nontarget tissues.AimsHere, we report that the pressure threshold for vaporization to induce cytotoxicity can be significantly reduced by selective intracellular delivery of PCNDs into targeted tumors.Methods and resultsIn vitro experiments revealed that selective intracellular delivery of PCNDs induced PCND aggregation specifically inside the targeted cells. This close‐packed configuration decreased the pressure threshold for vaporization to induce cytotoxicity. Moreover, following ultrasound exposure, significant decrease was observed in the viability of cells that incorporated PCNDs (35%) but not in the viability of cells that did not incorporate PCNDs (88%).ConclusionsIntracellular delivery of PCNDs reduced ultrasound pressure applied for vaporization to induce cytotoxicity. Confocal laser scanning microscopy and flow cytometry revealed that prolonged PCND‐cell incubation increased PCND uptake and aggregation. This aggregation effect might have contributed to the cytotoxicity threshold reduction effect.
Anthraquinone‐Based Oligomer as a Long Cycle‐Life Organic Electrode Material for Use in Rechargeable Batteries
Authors: Yao Masaru , Sano Hikaru , Ando Hisanori , Kiyobayashi Tetsu , Takeichi Nobuhiko 
Published: February 2019

AbstractAn anthraquinone (AQ)‐based dimer and trimer linked by a triple bond (−C≡C−) were newly synthesized as active materials for the positive electrode of rechargeable lithium batteries. These synthesized oligomers exhibited an initial discharge capacity of about 200 mAh g−1 with an average voltage of 2.2–2.3 V versus Li(C.E.). These capacity values are similar to that of the AQ‐monomer, reflecting the two‐electron transfer redox per AQ unit. Regarding their cycling stability, the capacity of the monomer electrode quickly decreased; however, the electrodes of the prepared oligomers showed an improved cycling performance. In particular, the discharge capacities of the trimer remained almost constant for 100 cycles. A theoretical calculation revealed that the intermolecular binding energy can be increased to the level of a weak covalent bonding by oligomerization, which would be beneficial to suppress the dissolution of the organic active materials into the electrolyte solutions. These results show that the cycle‐life of organic active materials can be extended without lowering the discharge capacity by the oligomerization of the redox active molecule unit.
Impact of disclosure and assurance quality of corporate sustainability reports on access to finance
Authors: García‐Sánchez Isabel‐María , Hussain Nazim , Martínez‐Ferrero Jennifer , Ruiz‐Barbadillo Emiliano 
Published: February 2019

AbstractThis paper investigates the impact of corporate social responsibility (CSR) disclosure quantity, quality, and external validation concerning assurance on capital constraints. We examine if these disclosure characteristics matter to the investors in the financial market, then they should be positively evaluated by financial market participants. More specifically, we study the effects of disclosure quantity, quality, and assurance on the access to financial resources for reporting firms. Analysis of data of an international sample for the period of 2007–2016 significantly supports the value relevance idea of CSR disclosure quality. We document that availability of more information about the firm's CSR initiatives eases the financial access. Furthermore, the quality and external assurance of CSR disclosure further strengthen the relationship between disclosure and access to finance. Our paper not only provides support for buying assurance but also argue for better assurance quality.
Benchmarking vdW‐DF first‐principles predictions against Coupled Electron–Ion Monte Carlo for high‐pressure liquid hydrogen
Authors: Gorelov Vitaly , Pierleoni Carlo , Ceperley David M. 
Published: February 2019

We report first‐principles results for the nuclear structure and optical responses of high‐pressure liquid hydrogen along two isotherms in the region of molecular dissociation. We employ density functional theory with the vdW‐DF approximation (vdW) and benchmark the results against existing predictions from Coupled Electron–Ion Monte Carlo (CEIMC). At fixed density and temperature, we find that the pressure obtained from vdW is higher than that from CEIMC by about 10 GPa in the molecular insulating phase and about 20 GPa in the dissociated metallic phase. Molecules are found to be over‐stabilized using vdW, with a slightly shorter bond length and with a stronger resistance to compression. As a consequence, pressure dissociation along isotherms using vdW is more progressive than that computed with CEIMC. Below the critical point, the liquid–liquid phase transition is observed with both theories in the same density region, but the one predicted by vdW has a smaller density discontinuity, i.e. a smaller first‐order character. The optical conductivity computed using Kubo–Greenwood formulation is rather similar for the two systems and reflects the slightly more pronounced molecular character of vdW.