bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nuclear envelope impairment is facilitated by the herpes simplex virus 1 Us3 kinase
Authors: Wild Peter , Leisinger Sabine , de Oliveira Anna Paula , Doehner Jana , Schraner Elisabeth M. , Fraevel Cornel , Ackermann Mathias , Kaech Andres 
Published: February 2019

Background: Capsids of herpes simplex virus 1 (HSV-1) are assembled in the nucleus, translocated either to the perinuclear space by budding at the inner nuclear membrane acquiring tegument and envelope, or released to the cytosol in a “naked” state via impaired nuclear pores that finally results in impairment of the nuclear envelope. The Us3 gene encodes a protein acting as a kinase, which is responsible for phosphorylation of numerous viral and cellular substrates. The Us3 kinase plays a crucial role in nucleus to cytoplasm capsid translocation. We thus investigate the nuclear surface in order to evaluate the significance of Us3 in maintenance of the nuclear envelope during HSV-1 infection.Methods: To address alterations of the nuclear envelope and capsid nucleus to cytoplasm translocation related to the function of the Us3 kinase we investigated cells infected with wild type HSV-1 or the Us3 deletion mutant R7041(∆Us3) by transmission electron microscopy, focused ion-beam electron scanning microscopy, cryo-field emission scanning electron microscopy, confocal super resolution light microscopy, and polyacrylamide gel electrophoresis.Results: Confocal super resolution microscopy and cryo-field emission scanning electron microscopy revealed decrement in pore numbers in infected cells. Number and degree of pore impairment was significantly reduced after infection with R7041(∆Us3) compared to infection with wild type HSV-1. The nuclear surface was significantly enlarged in cells infected with any of the viruses. Morphometric analysis revealed that additional nuclear membranes were produced forming multiple folds and caveolae, in which virions accumulated as documented by three-dimensional reconstruction after ion-beam scanning electron microscopy. Finally, significantly more R7041(∆Us3) capsids were retained in the nucleus than wild-type capsids whereas the number of R7041(∆Us3) capsids in the cytosol was significantly lower.Conclusions: The data indicate that Us3 kinase is involved in facilitation of nuclear pore impairment and, concomitantly, in capsid release through impaired nuclear envelope.
O cotidiano das aulas de Educação Física na Educação Infantil: os brinquedos em cena.
Authors: Costa João Augusto Galvão Rosa , Terra Dinah Vasconcellos 
Published: February 2019

Este trabalho parte do cotidiano da educação infantil de uma escola do Município de Duque de Caxias, localizada no Bairro de Gramacho. Discute um cenário despertado por alunos das turmas 51 e 52 da Educação Infantil durante as aulas de educação física escolar. Elegemos a observação participante como instrumento de coleta de dados. Tem como base teórica Michel Certeau, Tizuko Morchida Khishimoto, Gilles Brougère, Walter Benjamin, Paulo de Salles Oliveira, Johan Huizinga que nos orientaram nas reflexões, discussões acerca das ações apresentadas pelos alunos que se apropriaram desse objeto nas aulas, gerando categorias como a imaginação, a imitação, representação e reinvenção do brincar, pois o processo de (re) construção materializou diversos conteúdos dialogados no cotidiano das aulas.
AUTONOMIA DISCENTE NA UNIVERSIDADE: METODOLOGIAS ATIVAS E A CIBERCULTURA
Authors: Lupion Torres Patrícia , Carneiro Virginia Bastos , Trindade Fernandes Rui 
Published: February 2019

O presente artigo trata da autonomia discente em John Dewey e sua atualidade na prática das metodologias ativas no cenário educacional contemporâneo e na participação ativista em rede. O tema “Autonomia discente na universidade: metodologias ativas e a cibercultura” teve como objetivo geral refletir a perspectiva do desenvolvimento de um aluno autônomo com o aprender. Dentre os autores pesquisados estão TORRES, HILU, SIQUEIRA (2015); TRINDADE (2012). Pôde-se inferir que nas metodologias ativas hoje aplicadas em sala de aula, encontram-se pressupostos de autonomia discente em Dewey, na medida em que a valorização do aluno como centro do aprendizado no trabalho em grupos, além do trabalho individual, é estimulada em ambiente educacional democrático.Palavras-chave: autonomia discente; ambiente educacional democrático; metodologias ativas.
LIBERALISMO, ESTADO, COLONIALISMO E EDUCAÇAO: INTERFACES COM O PASSADO E AS LIÇÕES PARA A ATUALIDADE
Authors: Alencar Colares Anselmo , Imbiriba Sousa Colares Maria Lilia , Pires Barros Cardozo Maria José 
Published: February 2019

Este artigo discorre sobre algumas questões acerca do desenvolvimento do capitalismo e suas interfaces com o Estado a partir de estudos bibliográficos das obras A riqueza das nações, de Adam Smith e Princípios de economia política e tributação, de David Ricardo. Apresenta como esses autores revelam o pensamento liberal quanto a colonização e a intervenção do Estado. Conclui-se que a obra de David Ricardo, em especial, revela que várias de suas recomendações e assertivas quanto à defesa do liberalismo estão atuais, vivas e circulando vitoriosamente, inclusive no que se convencionou chamar de neoliberalismo. Portanto, considera-se pertinente refletir sobre estas questões, buscando compreender que as interfaces do Estado com a economia, são fundamentais para que possamos melhor compreender outros temas, entre os quais destacamos a educação.
Examining civic education pedagogies from a sociocultural curricular perspective: Lessons from three Israeli classrooms
Authors: Cohen Aviv 
Published: February 2019

Abstract
Research on pedagogical aspects of civic education is a field of study that deserves further exploration and scrutiny. This study focused on the examination of pedagogical practices enacted in three Israeli civics classrooms, in which an identical curriculum was taught. Classroom observations, teacher interviews and text analysis point to how despite similarities, each case presented different teaching activities and practices, framing and influencing the citizenship conceptions promoted in each case. These findings shed light on how pedagogy interplays with content while relating to contextual factors, determining and limiting the very notions of citizenship promoted in each case.
The Intangible Heritage of Ait Atta tribes in the Southern Morocco and Zemmour tribes in the Central Plateau
Authors: Lakhal Chaymae , ElKhabous Bouchra 
Published: February 2019

Most of Moroccan tribes were characterized by traveling in search of pastures to cater for the needs of cattle pasture. So, by their repetitive movements, it was caused some conflicts among themselves. It was only the results of practising the farming under the pressures of authorities. Thus, they began to know the kind of stability in the regions that they resorted to them. Despite this shift on the level of production systems, the conflict continues to fester over the resources, which were offered by the space and mainly represented in pastures and water, and the other as in the case with the tribes of Ait Atta and Zemmour. Consequently, they were prompted to put a Charter called the Tadda or Tatta in order to avoid disputes and conflicts and peacefully take advantage from economic resources, which are provided, by the space. Besides, the Tatta played an important role in stabilizing the peace and solidarity between tribes or between a family group tribe (subfraction) with another family group tribe. And, its implications extend to this present time. Furthermore, the Al-amazigh tribes retained those values that have become to reflect its identity as well as its heritage value which have made them cohesive despite the transformations that took place since the beginning of the 20th century. Due to this important subject, we will try to treat the importance of the Tada convention of Al-amazigh tribes and its impact on agricultural activity especially the grazing, in the tribes of south-east (Ait Atta) and the tribes of the central plateau (Zemmour) so as to view more on its position as well as its various geographical spaces. In this context, some questions arise in this principal issue, which we will try to answer later:

What is meant by the Tada convention?
What are the reasons for putting this convention?
What is its impact on agricultural activity especially grazing; on the relationships as well as the social ties with the unit spatial of tribes?
Immunoglobulin M and G in Virologically Confirmed Dengue Hemorrhagic Fever
Authors: Samsi Tatang K. , Wulur H. , Sugianto D. , Bartz C. R. , Tan R. , Sie A. 
Published: February 2019

Starting from September 1987, a one year prospective study on lgM and lgG in dengue hemorrhagic fever, was carried out at the Department of Pediatrics, Sumber War as Hospital West Jakarta. This report describes the preliminary finding of the study from September 1987 through June 1988.
Virus isolation and serologic analysis (HI, lgG and lgM capture ELISA) for DHF were done by NAMRU 2 in Jakarta. The subjects were 151 virologically confirmed DHF patients consisting of 82 boys and 69 girls of 6 months - 15 years old.
Serum samples were collected: (I) on the day of admission; (II) on the day of discharge and (Ill) 2 weeks after the first samples. Serum samples I, II, and Ill were collected from respectively 151, 131 and 64 subjects on respectively 3.5 ± 1.7; 8.8 ± 2.7 and 16.8 ± 2.1 days of illness. Positive lgM titer from acute sera was observed in 20% of subjects. A positive correlation between HI -lgM and HI -lgG was also observed.
The percentage of positive lgM titers rose with the increase of HI titer, the percentage of positive lgG titer was lower than that of /gM but a sudden increase exceeding that of lgM was observed at the HI liter of 320 and more.
This study revealed that HI titer of640 and 1280 were indicators for the primary and secondary dengue infection respectively and lgM capture ELISA can be used as a reliable predictor for DHF even more in fatal cases where only single serum is available.
Aplikasi biomaterial aktif dari daging ikan gabus (Channa striata) untuk penyembuhan luka pascaoperasi pada hewan model tikus Wistar
Authors: Sunarno Sunarno , Damayanti Rahayu , Devi Alfisa , Fikri Muhammad , Pratiwi F , Ayu Linda 
Published: February 2019

One problem for postoperative patients is tissue recovery takes a long time due to the occurrence of blood clotting disorders and immune system. Snakehead fish was known to contain bioactive ingredients that can potentially speed up the process of tissue repair as a result of injuries. Snakehead fish meat contains a lot of minerals, albumin, and amino acids needed for tissue protein synthesis and increases the antioxidant glutathione. This study was conducted to test the extraction of snakehead fish flesh against skin tissue wound healing in Wistar rats. The study was conducted with a completely randomized design consisting of three treatments with 6 replication, which include (P0) a negative control (without treatment madecassol and snakehead fish meat extract), (P1) madecassol commercial drug treatment, and (P2) treatment of snakehead fish meat extract. Each material was injected intramuscularly in Wistar rats at a dose of 9 ml/kg bb for 7 days. Data analysis uses Anova followed by test Honestly Significant Difference (HSD) with a significance of 95%. Treatment of snakehead meat extract with a dose of 9 ml/kg bb a significant effect on skin tissue repair after injury, better than treatment with madecassol and control with the average value of extensive tissue remaining after recovering from the condition of the wound by 0.2 cm2; 0.33 cm2; and 0.4 cm2, respectively. Snakehead fish meat extract with a dose of 9 ml/kg bb injected intramuscularly can accelerate the recovery of tissue in the skin after the wound in Wistar rats.
Replication of R-pronouns in German dialects
Authors: Hein Johannes , Barnickel Katja 
Published: February 2019

Abstract
A considerable number of German dialects exhibit doubled R-pronouns with pronominal adverbs
(dadamit, dadafür, dadagegen). At first sight, this type of in situ
replication seems to be completely redundant since its occurrence is independent of R-pronoun extraction/movement.
The main purpose of this paper is to account for (i) the difference between dialects with regard to replication
of R-pronouns and (ii) why an (apparently redundant) process of replication occurs. Following Müller (2000a),
who considers R-pronouns to be a repair phenomenon, we present an analysis in the framework of
Optimality Theory. We argue that replication of R-pronouns is a consequence of different rankings of
universal requirements like e.g. the Inclusiveness Condition, the Lexical Integrity
Hypothesis and Antilocality and that the interaction of these constraints results
in the occurrence of replication.
Going with the Flow: The Neural Mechanisms Underlying Illusions of Complex-Flow Motion
Authors: Luo Junxiang , He Keyan , Andolina Ian Max , Li Xiaohong , Yin Jiapeng , Chen Zheyuan , Gu Yong , Wang Wei 
Published: February 2019

Studying the mismatch between perception and reality helps us better understand the constructive nature of the visual brain. The Pinna–Brelstaff motion illusion is a compelling example illustrating how a complex moving pattern can generate an illusory motion perception. When an observer moves toward (expansion) or away (contraction) from the Pinna–Brelstaff figure, the figure appears to rotate. The neural mechanisms underlying the illusory complex-flow motion of rotation, expansion, and contraction remain unknown. We studied this question at both perceptual and neuronal levels in behaving male macaques by using carefully parametrized Pinna–Brelstaff figures that induce the above motion illusions. We first demonstrate that macaques perceive illusory motion in a manner similar to that of human observers. Neurophysiological recordings were subsequently performed in the middle temporal area (MT) and the dorsal portion of the medial superior temporal area (MSTd). We find that subgroups of MSTd neurons encoding a particular global pattern of real complex-flow motion (rotation, expansion, contraction) also represent illusory motion patterns of the same class. They require an extra 15 ms to reliably discriminate the illusion. In contrast, MT neurons encode both real and illusory local motions with similar temporal delays. These findings reveal that illusory complex-flow motion is first represented in MSTd by the same neurons that normally encode real complex-flow motion. However, the extraction of global illusory motion in MSTd from other classes of real complex-flow motion requires extra processing time. Our study illustrates a cascaded integration mechanism from MT to MSTd underlying the transformation from external physical to internal nonveridical flow-motion perception.SIGNIFICANCE STATEMENTThe neural basis of the transformation from objective reality to illusory percepts of rotation, expansion, and contraction remains unknown. We demonstrate psychophysically that macaques perceive these illusory complex-flow motions in a manner similar to that of human observers. At the neural level, we show that medial superior temporal (MSTd) neurons represent illusory flow motions as if they were real by globally integrating middle temporal area (MT) local motion signals. Furthermore, while MT neurons reliably encode real and illusory local motions with similar temporal delays, MSTd neurons take a significantly longer time to process the signals associated with illusory percepts. Our work extends previous complex-flow motion studies by providing the first detailed analysis of the neuron-specific mechanisms underlying complex forms of illusory motion integration from MT to MSTd.
Review of the Housing Policies and Programmes in Nigeria
Authors: Bello Aminu 
Published: February 2019

Problem of inadequate housing faced by poor people around the world has been a matter of concern to governments in various countries especially in the developing world whereby significant portion of the population is mostly characterized by poverty. In such countries, the housing problem is not only that of quantity but also of the poor quality of available housing units. Private sector development in the Nigerian housing sector has been a standstill for more than a decade. With few exceptions, the private sector transactions that have taken place have been informal and on the fringe of legality. At the opposite of the spectrum, public sector activity is plagued with many problems. Instead of operating as a social policy, it operates more like a regressive lottery or patronage system. The results have been the simultaneous construction of some of the most luxurious subsidized housing in Africa, and general deterioration in housing conditions of most Nigerians, particularly the housing conditions of the poor. The recorded history of formal intervention into the housing sector in Nigeria dated back to the colonial administration, after the unfortunate outbreak of the bubonic plaque of 1928 in Lagos. This necessitated the establishment of the Lagos Executive Development Board (LEDB). This signifies the ushering of Nigerian public housing programmes intervention; which was during colonial era. The policies are modest with the ultimate aim of addressing the housing problem at a National scale. The policy focus then, was on the provision of expatriate quarters and some selected indigenous staff in Rail ways, Marine, Police and Armed forces. The construction of senior civil servant quarters in the capital city of Lagos and regional headquarters like Kaduna, Ibadan and Enugu are some of the practical efforts made at the same time some form of rent subsidy and housing loans.
Ağrı Kliniğine Başvuran Kronik Ağrılı Hastaların Geriye Dönük Değerlendirilmesi: İki Yıllık Deneyimlerimiz
Authors: ARICI Tülin , KILIÇ Ertuğrul 
Published: February 2019

Giriş ve Amaç:Bu çalışmadaki amacımız, hastanemizde ilk defa açılan ağrı polikliniğine ilk 2 yılda ağrı şikayeti ile başvuran hastaların ağrı tipleri, demografik özellikleri ile tedavilerini geriye dönük olarak değerlendirmektir. Gereç ve Yöntem: Çalışmamızda ocak 2016-aralık 2017 tarihleri arasındaXXXX eğitim ve Araştırma hastanesi ağrı polikliniğine kronik ağrı şikayeti ile başvuran hastaların dosyaları incelendi. Hastaların ağrı tipleri, demografik özellikleri ve uygulanan tedaviler geriye dönük olarak değerlendirildi. Bulgular: İki yıllık sürede polikliniğimize 6545 kronik ağrılıhasta başvurduğu görüldü.Hastaların 4199’u kadın (%64.15), 2346’sı erkek (%35.85) olarak tespit edildi. En sık kanser dışı ağrı nedenleri (KDA); bel ağrısı, myofasial ağrıvenöropatik ağrı idi. Kanser ağrısı (KA) nedeni ile başvuran hasta sayısı 358 (%5.46) olup en sık nedenler gastrointestinal sistem kaynaklı kanser, akciğer kanseri ve meme kanseri idi. Hastaların tamamına medikal tedavi ve 728 (%11.12) hastaya ek olarak girişimsel ağrı tedavisi uygulandığı tespit edildi.En sık yapılan girişimsel ağrı tedavi yöntemleri; tetik nokta enjeksiyonu, faset sinir radyofrekans termokoagülasyon uygulaması, epidural steroid uygulamaları ve dorsal kök ganglion radyofrekans uygulaması olarak saptandı. Sonuç: Ağrı klinikleri ve başvuran hasta sayısı devamlı olarak artmaktadır. Hastanemizde 2 yıl önce kurulan ve yüksek nüfus bölgelerine hitap eden merkezimizin bu sonuçlarının kronik ağrı çalışmaları için önemli bir veri kaynağı olacağını düşünmekteyiz.
Penelitian dan Pengembangan Material Struktur Reaktor Maju
Authors: Aziz Ferhat 
Published: February 2019

PENELITIAN DAN PENGEMBANGAN MATERIAL STRUKTUR REAKTOR MAJU. Pengoperasian suatu pembangkit listrik tenaga nuklir secara selamat dan ekonomis sangat tergantung kepada ketersediaan material struktur yang handal. Selama umur operasi reaktor yang dewasa ini mencapai 60 tahun, material struktur terpapar temperatur yang tinggi, lingkungan korosif dan medan radiasi kuat yang terjadi akibat proses fisi. Struktur penopang bahan bakar akan memiliki umur lebih singkat dalam lingkungan demikian. Sementara itu tuntutan dalam pengembangan reaktor maju masa depan adalah temperatur operasi dan burnup yang lebih tinggi untuk meningkatkan efisiensi operasi reaktor. Material yang mampu mengatasi kondisi itu harus stabil secara dimensional dalam medan radiasi, baik dengan maupun tanpa adanya stress, serta memiliki sifat yang baik di lingkungan yang korosif. Makalah ini menelaah secara singkat litbang material struktur reaktor yang potensial digunakan dalam sistem reaktor maju, seperti baja ferritic/martensitic, baja ODS, dan keramik, termasuk sintesis baja ODS yang dikembangkan di BATAN. Selain itu dikaji pula kerusakan radiasi pada material struktur serta sintesis dan simulasi kinerja perisai radiasi menggunakan paket program MCNP5. Hasil kajian menunjukkan bahwa walaupun hasil yang ada sudah cukup baik, masih diperlukan litbang lebih lanjut guna mendapatkan material yang memenuhi kriteria yang diinginkan, baik melalui eksperimen maupun secara simulasi dan pemodelan dengan komputer.
Strategi Pemeliharaan Komponen pada Sistem Pendingin RSG-GAS Berdasarkan Estimasi Interval Waktu Perawatan
Authors: Hartini Entin 
Published: February 2019

STRATEGI PEMELIHARAAN KOMPONEN PADA SISTEM PENDINGIN RSG-GAS BERDASARKAN ESTIMASI INTERVAL WAKTU PERAWATAN. Proses penuaan akan menyebabkan penurunan keandalan dan kinerja reaktor, oleh karena itu diperlukan pemeliharaan sistem/komponen reaktor yang optimal. Pemeliharaan korektif terhadap sistem/komponen berdampak pada frekuensi kerusakan dan biaya perawatan yang tinggi. Tujuan dari penelitian ini adalah melakukan proses pendekatan manajemen keandalan dengan melakukan perencanaan interval pemeliharaan. Skenario pemeliharaan dapat dilakukan berdasarkan penggantian komponen sesuai dengan mean time to failure (MTTF) dan ketika keandalan komponen memenuhi presentase yang ditargetkan. Metodologi yang digunakan adalah uji distribusi data dan estimasi parameter untuk menetukan interval waktu perawatan dan keandalan komponen. Data yang dievaluasi adalah data perawatan komponen dari sistem pendingin RSG-GAS teras 81 sampai 94 tahun 2013-2017. Hasil pengolahan data menunjukkan bahwa untuk meminimalkan jumlah downtime berdasarkan interval waktu perawatan dan nilai keandalan komponen, maka strategi perawatan yang dapat dilakukan adalah untuk komponen Pompa Primer (JE-01 (AP01-02)) interval perawatan 245,27 hari dengan peluang keandalan komponen (R(t)) = 35,2%. Untuk komponen Instrumentasi Pengukuran Aktivitas γ (PA01-02/CR001) interval perawatan 203,57 hari dengan peluang keandalan komponen (R(t)) = 51,1%. Sedangkan jika diinginkan keandalan komponen sebesar 60% maka dapat dilakukan interval waktu perawatan 144,23 hari untuk komponen JE-01 (AP01-02) dan 160,35 hari untuk komponen PA01-02/CR001.Kata kunci: keandalan, perawatan, sistem pendingin, RSG-GAS
OVERNIGHT COST ESTIMATION OFINDONESIA’S COGENERATION POWER PLANT FOR INDUSTRIAL PROCESSES
Authors: Setya Budi Rizki Firmansyah , Rijanti Arum Puni , Lumbanraja Sahala M. , Amitayani Elok S. , Birmano Moch. Djoko , Liun Edwaren 
Published: February 2019

Indonesia’s cogeneration power plant for industrial processes (PeLUIt) is needed to be implemented in Indonesia. Economic analysis is an important factor for PeLUIt implementation. One of the data that needs in the analysis is overnight cost. This research estimates HTGR’s overnight cost by using scaling law.By using the estimated value, it can be calculated the PeLUIt overnight cost. The research’s purpose is to obtain an estimation of overnight costs of PeLUIt. The estimation result shows the overnight cost of PeLUIt. PeLUIt 10 MWth has an overnight cost 166,26 million USD. PeLUIt 30 MWth has an overnight cost 233,49 million USD. PeLUIt 50 MWth has an overnight cost 281,31 million USD. PeLUIt 100 MWth has an overnight cost 371,86 million USD. PeLUIt 350 MWth has an overnight cost 657,16 million USD.Keywords: PeLUIt; Estimation; High temperature gas-cooled reactor; Overnight cost; Scaling law