bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Vitro Trypanocidal and Antibacterial Activities of Essential Oils from Four Species of the Family Annonaceae
Authors: Bay Marcia , Souza de Oliveira João Vitor , Sales Junior Policarpo Ademar , Fonseca Murta Silvane Maria , Rogério dos Santos Anderson , dos Santos Bastos Ivanildes , Puccinelli Orlandi Patrícia , Teixeira de Sousa Junior Paulo 
Published: September 2019

AbstractThe objective of this study was to evaluate the chemical composition, and the trypanocidal and antibacterial activities of the essential oils from four species of Annonaceae: Bocageopsis multiflora (Mart.) R.E.Fr., Duguetia quitarensis Benth., Fusaea longifolia (Aubl.) Saff., and Guatteria punctata (Aubl.) R.A.Howard. The chemical composition of the essential oils from the aerial parts yielded 23, 20, 21 and 23 constituents, respectively, which were identified by GC/MS. The trypanocidal activity was evaluated against the amastigote and trypomastigote forms of T. cruzi. The antibacterial activity was evaluated by the microdilution method against enterohemorrhagic Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, Streptococcus pyogenes, and methicillin‐resistant Staphylococcus aureus. The results of trypanocidal activity showed that the essential oils of the four species were active at the tested concentrations, with G. punctata essential oil being the most active, with IC50=0.029 μg/mL, and selectivity index (SI)=32, being 34 times more active than the reference drug benznidazole. All EOs showed strong antibacterial activity (minimum inhibitory concentrations of 4.68–37.5 μg/mL) against strains of S. mutans.
Thermodynamic and Structural Behavior of α‐Galactosylceramide and C6‐Functionalized α‐GalCer in 2D Layers at the Air–Liquid Interface
Authors: Brezesinski Gerald , Calow Adam D. J. , Pereira Claney L. , Seeberger Peter H. 
Published: September 2019

Abstractα‐Galactosylceramide (α‐GalCer; KRN7000) is a ligand for the glycoprotein CD1d that presents lipid antigens to natural killer T cells. Therefore, KRN7000 as well as some modified versions thereof have been widely investigated as part of novel immunotherapies. To examine the impact of structural modification, we investigated KRN7000 and C6‐modified KRN7000 at the air–liquid interface using monolayer isotherms, BAM, IRRAS, GIXD, and TRXF. The amino group has no influence on the highly ordered sub‐gel structures found at lateral pressures relevant for biological membranes. Neither lateral compression nor the protonation state of the amino group has a measurable effect on the lattice structure, which is defined by strong and rigid intermolecular hydrogen bonds. However, the first‐order phase transition found for the C6‐functionalized α‐GalCer is connected with an extraordinary surface‐inhibited nucleation. Our study demonstrates that KRN7000 can be functionalized at C6 without significantly changing the structural properties.
Sulfate Surfactant Assisted Approach to Fabricate Sulphur‐Doped Supported Nanodiamond Catalyst on Carbon Nanotube with Unprecedented Catalysis for Ethylbenzene Dehydrogenation
Authors: Zhou Qin , Zhao Zhongkui 
Published: September 2019

AbstractDirect dehydrogenation (DDH) of ethylbenzene is an efficient chemical process for styrene production. Nanocarbon materials, as clean and low‐cost catalysts, have drawn more and more attention in this field. In this work, inspired by the promoting effect of support modulation on the catalytic performance of the general supported‐type catalyst, we present a facile sulfate surfactant assisted dispersion with subsequent annealing (SADA) approach for preparing sulphur‐doped supported nanodiamond catalyst on carbon nanotube (ND/CNT‐SDS) with abundant active sites by using sodium dodecyl sulfate (SDS) as both the dispersing agent for promoting the dispersion of catalytically active ND motif and the CNT support and the sulphur source for sulphur doping, yielding an outstanding supported ND catalyst for DDH of ethylbenzene to styrene with 7.7 mmol g−1cat. h−1 of styrene rate and 98.2 % of styrene selectivity. ND/CNT‐SDS catalyst demonstrates 3.0 times higher styrene rate of the pristine ND. Moreover, ND/CNT‐SDS catalyst shows 1.5 times higher styrene rate of the previously reported defect‐enriched N,O‐codoped ND/CNT (N,O‐ND/CNT‐d) with up to 98 % of similar styrene selectivity, ascribed to its more exposed C=O active sites and higher specific surface area originating from the promoted dispersion of both ND and CNT by SDS, as well as the sulphur‐doping. Moreover, this work also presents a novel and efficient method for the designing the other supported nanocarbon catalysts from dispersion‐required catalytically active nanocarbon motif and the nanocarbon carrier.
Binary Lead Fluoride Pb3F8
Authors: Deubner H. Lars , Sachs Malte , Bandemehr Jascha , Ivlev Sergei I. , Karttunen Antti J. , Kachel Stefan R. , Klein Benedikt P. , Ruppenthal Lukas , Schöniger Maik , Krug Claudio K. , Herritsch Jan , Gottfried J. Michael , Aman Jamal N. M. , Schmedt auf der Günne Jörn , Kraus Florian 
Published: September 2019

AbstractThe binary lead fluoride Pb3F8 was synthesized by the reaction of anhydrous HF with Pb3O4 or by the reaction of BrF3 with PbF2. The compound was characterized by single‐crystal and powder X‐ray diffraction, IR, Raman, and solid‐state MAS 19F NMR spectroscopy, as well as thermogravimetric analysis, XP and near‐edge X‐ray absorption fine structure (NEXAFS) spectroscopy. Solid‐state quantum‐chemical calculations are provided for the vibrational analyses and band assignments. The electronic band structure offers an inside view of the mixed valence compound.
One‐Pot Synthesis of Xanthate‐Functionalized Cellulose for the Detection of Micromolar Copper(II) and Nickel(II) Ions
Authors: Greis Kim , Bethke Kevin , Stückrath Julius B. , Ingber Tjark T. K. , Valiyaveettil Suresh , Rademann Klaus 
Published: September 2019

AbstractA one‐pot synthesis and application of cellulose‐based sensors to efficiently detect various toxic metal ions in aqueous solutions in micromolar quantities is reported. Cellulose microfibers have been functionalized with carbon disulfide in alkaline solution to form cellulose xanthate. The material detects several toxic metal ions such as copper, nickel, or cobalt ions through color change detectable by the naked eye. The optical sensor can be used as an ideal flash test for assessing the quality of drinking water.
Health‐related quality of life in oncology drug reimbursement submissions in Canada: A review of submissions to the pan‐Canadian Oncology Drug Review
Authors: Raymakers Adam J. N. , Regier Dean A. , Peacock Stuart J. 
Published: September 2019

BackgroundIn Canada, the Canadian Agency for Drugs and Technologies in Health (CADTH) evaluates and makes recommendations for the reimbursement of cancer drugs. One component of its recommendation is based on an economic evaluation, which typically takes the form of a cost‐utility analysis. A cost‐utility analysis measures the effects of competing therapies with quality‐adjusted life‐years (QALYs). The data for this calculation typically come from generic, preference‐based measures of health‐related quality of life (HRQOL). The objective of this review is to determine the frequency at which HRQOL data are collected alongside cancer drug trials and used in the cost‐utility analysis submitted to the CADTH pan‐Canadian Oncology Drug Review (pCODR).MethodsSubmissions between 2015 and 2018 to pCODR, the group charged with evaluating cancer drug submissions at CADTH, were reviewed. All pCODR submissions, either in progress or completed, were publicly available online. The search was restricted to completed evaluations.ResultsForty‐three submissions met the inclusion criteria. The incremental gain in QALYs in most submissions from the new technology was small (median incremental gain, 0.86; interquartile range, 0.6‐1.39). More than half of the submissions (56%) did not include original data on HRQOL, with most relying on previous studies of variable relevance and quality. Re‐analyses by pCODR based on concerns over HRQOL data used in the submitted model were common (52%).ConclusionsDrug manufacturers do not consistently collect data on HRQOL alongside clinical trials and instead rely on evidence generated in previous studies to inform cost‐utility analyses. These findings should induce manufacturers to collect original HRQOL data that are simultaneously relevant to patients and decision makers.
Evaluation of CO2 attack in wellbore class G cement: influence of epoxy resins, composites and minerals as additives
Authors: Schütz Marta K. , dos Santos Leonardo M. , Coteskvisk Patrícia M. , Menezes Sonia C. , Einloft Sandra , Dalla Vecchia Felipe 
Published: September 2019

AbstractCarbon dioxide (CO2) injection into geological formations is pointed out as one of the most effective alternatives to reduce anthropogenic CO2 emissions to the atmosphere. To promote long‐term CO2 storage, wellbore integrity is a critical issue to be considered. Portland cement is commonly used for cementing wells, and considered chemically unstable in CO2‐rich media. In this context, this study investigated the CO2 chemical resistance of class G Portland cement modified with novel additives (epoxy resins, epoxy–clay composites, and clay minerals) at 1 and 2.5 wt% contents. Reaction times of 7 and 30 days of exposure to CO2 in supercritical conditions were evaluated. Samples were characterized by mechanical compression tests and phenolphthalein indicator as well as field emission scanning electron microscopy in order to determine the depth of carbonation in cement. Our results indicate that although there is slight reduction in the initial compressive strength, the addition of tested additives to cement paste offers improvements in terms of chemical resistance. The optimum content of different additives was 1 wt% in order to maintain compressive strength properties and improve chemical resistance to CO2. The best result was achieved with an epoxy resin blend as an additive, decreasing carbonation by up to 60% (7 days of exposure to CO2) and 52% (30 days of exposure to CO2). Addition of montmorillonite to the epoxy blend tends to improve chemical resistance of cement paste when compared to the neat epoxy blend. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd.
LRP6 regulates Rab7‐mediated autophagy through the Wnt/β‐catenin pathway to modulate trophoblast cell migration and invasion
Authors: Li Lei , Peng Wei , Zhou Qian , Wan Ji‐Peng , Wang Xie‐Tong , Qi Hong‐Bo 
Published: September 2019

AbstractPre‐eclampsia is a common complication during pregnancy; however, the underlying mechanisms of the crosstalk between low‐density lipoprotein receptor‐related protein 6 (LRP6) and autophagy in trophoblast cells are still not fully explored. Messenger RNA (mRNA) and protein levels of LRP6, beclin 1, Unc‐51‐like autophagy activating kinase 1 (ULK1), p62, vimentin, matrix metallopeptidase‐9 (MMP‐9), β‐catenin, c‐Myc, and Rab7, as well as the ratio of LC3‐II/LC3‐I, were analysed by quantitative real‐time polymerase chain reaction or Western blot analysis, respectively. An MTT assay was used to measure cell growth, and transwell and wound healing assays were carried out to evaluate the invasion and migration abilities of the trophoblasts used. An immunofluorescence assay was used to measure LC3. The mRFP‐GFP‐LC3 tandem fluorescence assay was applied to detect autophagic flow. LRP6 overexpression was achieved by constructing pcDNA3.1‐LRP6 vectors. LRP6 was expressed at low levels in HTR‐8/SVneo cells under hypoxia/reoxygenation (H/R) conditions. H/R inhibited the activation of autophagy. LRP6 overexpression promoted cell proliferation and activated autophagy, which led to the upregulation of beclin 1 and ULK1, as well as the ratio of LC3‐II/LC3‐I and the downregulation of p62. Furthermore, LRP6 overexpression elevated the migration and invasion abilities of the indicated cells and increased vimentin and MMP‐9 expression levels. Furthermore, LRP6 upregulated Rab7 and activated autophagy through the Wnt/β‐catenin pathway. The late autophagy inhibitor bafilomycin A1 (Baf‐A1) and the Wnt/β‐catenin pathway inhibitor PKF115‐584 reversed the effects of LRP6 on trophoblast autophagy, migration and invasion. LRP6 promotes Rab7‐mediated autophagy by activating the Wnt/β‐catenin pathway, which leads to increasing migration and invasion of trophoblast cells. Our study paves a new avenue for clinical treatment, and LRP6 may serve as an essential target in pre‐eclampsia.
Developmental transitions during adulthood and neighborliness: A multilevel cluster analysis
Authors: Aiyer Sophie M. , Zahnow Renee , Mazerolle Lorraine A. 
Published: September 2019

AbstractNeighborliness plays a critical role in promoting social integration, and is known to positively influence health and psychosocial adjustment. We examined variation in neighborliness based on developmental transitions as well as on the neighborhood context. We examined the direct and moderating role of neighborhood factors, to determine whether the neighborhood context modified the influence of developmental transitions on neighborliness. We analyzed data from a longitudinal study of Australian communities, in addition to census data. First, we analyzed developmental transition clusters. Next, we employed multilevel modeling to assess the impact of clusters and other key factors on neighborliness. We tested interactions to determine whether the influence of cluster membership on neighborliness was modified by the neighborhood. We found evidence for direct effects of cluster membership and structural factors on neighborliness. In addition, the neighborhood context modified the influence of cluster membership on neighborliness. Our findings underscore the importance of promoting neighborhood social engagement throughout the life course.
The BioLuminescent‐OptoGenetic in vivo response to coelenterazine is proportional, sensitive, and specific in neocortex
Authors: Gomez‐Ramirez Manuel , More Alexander I. , Friedman Nina G. , Hochgeschwender Ute , Moore Christopher I. 
Published: September 2019

AbstractBioLuminescent (BL) light production can modulate neural activity and behavior through co‐expressed OptoGenetic (OG) elements, an approach termed “BL‐OG.” Yet, the relationship between BL‐OG effects and bioluminescent photon emission has not been characterized in vivo. Further, the degree to which BL‐OG effects strictly depend on optogenetic mechanisms driven by bioluminescent photons is unknown. Crucial to every neuromodulation method is whether the activator shows a dynamic concentration range driving robust, selective, and nontoxic effects. We systematically tested the effects of four key components of the BL‐OG mechanism (luciferin, oxidized luciferin, luciferin vehicle, and bioluminescence), and compared these against effects induced by the Luminopsin‐3 (LMO3) BL‐OG molecule, a fusion of slow burn Gaussia luciferase (sbGLuc) and Volvox ChannelRhodopsin‐1 (VChR1). We performed combined bioluminescence imaging and electrophysiological recordings while injecting specific doses of Coelenterazine (substrate for sbGluc), Coelenteramide (CTM, the oxidized product of CTZ), or CTZ vehicle. CTZ robustly drove activity in mice expressing LMO3, with photon production proportional to firing rate. In contrast, low and moderate doses of CTZ, CTM, or vehicle did not modulate activity in mice that did not express LMO3. We also failed to find bioluminescence effects on neural activity in mice expressing an optogenetically nonsensitive LMO3 variant. We observed weak responses to the highest dose of CTZ in control mice, but these effects were significantly smaller than those observed in the LMO3 group. These results show that in neocortex in vivo, there is a large CTZ range wherein BL‐OG effects are specific to its active chemogenetic mechanism.
Characterization of chemical constituents and absorbed components, screening the active components of gelanxinning capsule and an evaluation of therapeutic effects by ultra‐high performance liquid chromatography with quadrupole time of flight mass spectrometry
Authors: Gao Xin , Hu Xiaohu , Zhang Qiong , Wang Xijing , Wen Xiuhong , Wang Yuan , Zhang Yanxia , Sun Wenjun 
Published: September 2019

AbstractWe revealed the potential biomarker and pathway of gelanxinning capsule on rat model with coronary heart disease, which aims to clarify holistic therapeutic effect and predict quality‐markers of gelanxinning capsule. Ultra‐high performance liquid chromatography coupled with mass spectrometry based on metabolomics technique was used to find the biomarkers and related metabolic pathways of coronary heart disease model, which evaluates the intervention effect of gelanxinning capsule. Using serum pharmacochemistry of traditional Chinese medicine and Pearson correlation analysis, effective ingredients in serum is analyzed to characterize the activity of gelanxinning capsule on coronary heart disease under valid state. A total of 20 biomarkers from coronary heart disease were identified and 12 of them were regulated by gelanxinning capsule treatment, which is mainly involved in sphingolipid metabolism and glycerophospholipid metabolism. With the high sensitivity liquid chromatography coupled with mass spectrometry technology, a total of 46 compounds from gelanxinning capsule were identified in vitro and 25 of them were absorbed in blood. The correlation analysis of serum biomarkers and absorbed components was used to find 11 compounds as quality‐markers to be responsible for the efficacy of gelanxinning capsule. This strategy was successfully applied to screening of potential mechanism and quality‐markers from herbal medicine.
Regio‐ and Stereospecific Cyclopolymerization of α,ω‐Diynes by Cationic Molybdenum Imido Alkylidene N‐Heterocyclic Carbene Complexes
Authors: Probst Patrick , Elser Iris , Schowner Roman , Benedikter Mathis J. , Buchmeiser Michael R. 
Published: September 2019

AbstractBoth solvent‐free and acetonitrile‐containing cationic molybdenum imido alkylidene N‐heterocyclic carbene (NHC) complexes of the general formula [Mo(NR′)(CHCMe2R)(NHC)(X)+ A−] (R′ = 2,6‐Cl2‐C6H3, tBu, 2‐CF3‐C6H4, 2‐tBu‐C6H4, 2,6‐iPr2‐C6H3, 2,6‐Me2‐C6H3; R = Me, Ph; NHC = 1,3‐dimesitylimidazol‐2‐ylidene (IMes), 1,3‐di‐iPr‐imidazol‐2‐ylidene (IPr), 1,3,5‐triphenyl‐1,3,4‐triazol‐2‐ylidene); X = CF3SO3, C6F5O, OCH(CF3)2, OC(CF3)3, pyrrolide, C6F5COO, 2,6‐(CF3)2‐C6H3COO; A− = B(ArF)4−, Al(OC(CF3)3)4−), have been investigated for their propensity to cyclopolymerize 4,4‐disubstituted 1,6‐heptadiynes. All metal complexes contain a stereogenic (chiral) metal center, which accounts for the high reactivity and high regioselectivity of insertion (>99%) that are observed for all metal complexes, leading to highly conjugated, α‐insertion‐derived polyenes that are based on a highly regular polymer backbone and that show absorption maxima close to 600 nm. With the chiral monomer 4‐(ethoxycarbonyl)‐4‐(1S,2R,5S)‐(‐)‐menthoxycarbonyl‐1,6‐heptadiyne, high syndiospecifity (>99% syndiotactic) is observed. A mechanism explaining the high regio‐ and stereoselectivity is presented. Thus, α‐addition of the monomers proceeds chain‐end‐controlled trans to the NHC and is preferred over β‐addition through intramolecular Mo‐O chelation. Insertion of the monomers entails double inversion at the stereogenic metal center in the course of one complete monomer insertion.
Heat‐induced shift in coral microbiome reveals several members of the Rhodobacteraceae family as indicator species for thermal stress in Porites lutea
Authors: Pootakham Wirulda , Mhuantong Wuttichai , Yoocha Thippawan , Putchim Lalita , Jomchai Nukoon , Sonthirod Chutima , Naktang Chaiwat , Kongkachana Wasitthee , Tangphatsornruang Sithichoke 
Published: September 2019

AbstractThe coral holobiont is a complex ecosystem consisting of coral animals and a highly diverse consortium of associated microorganisms including algae, fungi, and bacteria. Several studies have highlighted the importance of coral‐associated bacteria and their potential roles in promoting the host fitness and survival. Recently, dynamics of coral‐associated microbiomes have been demonstrated to be linked to patterns of coral heat tolerance. Here, we examined the effect of elevated seawater temperature on the structure and diversity of bacterial populations associated with Porites lutea, using full‐length 16S rRNA sequences obtained from Pacific Biosciences circular consensus sequencing. We observed a significant increase in alpha diversity indices and a distinct shift in microbiome composition during thermal stress. There was a marked decline in the apparent relative abundance of Gammaproteobacteria family Endozoicomonadaceae after P. lutea had been exposed to elevated seawater temperature. Concomitantly, the bacterial community structure shifted toward the predominance of Alphaproteobacteria family Rhodobacteraceae. Interestingly, we did not observe an increase in relative abundance of Vibrio‐related sequences in our heat‐stressed samples even though the appearance of Vibrio spp. has often been detected in parallel with the increase in the relative abundance of Rhodobacteraceae during thermal bleaching in other coral species. The ability of full‐length 16S rRNA sequences in resolving taxonomic uncertainty of associated bacteria at a species level enabled us to identify 24 robust indicator bacterial species for thermally stressed corals. It is worth noting that the majority of those indicator species were members of the family Rhodobacteraceae. The comparison of bacterial community structure and diversity between corals in ambient water temperature and thermally stressed corals may provide a better understanding on how bacteria symbionts contribute to the resilience of their coral hosts to ocean warming.