bluuebunny/crossref_metadata_2025_split huggingface dataset:
No Evidence for Milankovitch Cycle Influence on Abyssal Hills at Intermediate, Fast, and Superfast Spreading Rates
Authors: Goff John A. , Zahirovic Sabin , Müller R. Dietmar 
Published: August 2018

AbstractA recent hypothesis contends that abyssal hill topography is linked to sea level periodicities expressed by Milankovitch cycles, predicting that abyssal hill elevation is correlated to crustal age. We test this prediction by stacking (averaging) bathymetry as a function of age to enhance age‐dependent signal while suppressing random (primarily faulted) components. Stacking is applied to bathymetry data flanking intermediate, fast, and superfast spreading ridges. Revised digital crustal age models were generated in these regions using a recent compilation of reliable magnetic anomaly identifications, with inferred temporal uncertainty of ~0.01 my. We utilize statistical properties of abyssal hills to predict the variability of the age‐stack under the null hypothesis that abyssal hills are random with respect to crustal age; the age‐stacked profile is significantly different from zero only if it exceeds this expected variability by a large margin. Our results do not support the presence of Milankovitch‐driven signals in abyssal hill topography.
A Comparison of Methods for Streamflow Uncertainty Estimation
Authors: Kiang Julie E. , Gazoorian Chris , McMillan Hilary , Coxon Gemma , Le Coz Jérôme , Westerberg Ida K. , Belleville Arnaud , Sevrez Damien , Sikorska Anna E. , Petersen‐Øverleir Asgeir , Reitan Trond , Freer Jim , Renard Benjamin , Mansanarez Valentin , Mason Robert 
Published: August 2018

AbstractStreamflow time series are commonly derived from stage‐discharge rating curves, but the uncertainty of the rating curve and resulting streamflow series are poorly understood. While different methods to quantify uncertainty in the stage‐discharge relationship exist, there is limited understanding of how uncertainty estimates differ between methods due to different assumptions and methodological choices. We compared uncertainty estimates and stage‐discharge rating curves from seven methods at three river locations of varying hydraulic complexity. Comparison of the estimated uncertainties revealed a wide range of estimates, particularly for high and low flows. At the simplest site on the Isère River (France), full width 95% uncertainties for the different methods ranged from 3 to 17% for median flows. In contrast, uncertainties were much higher and ranged from 41 to 200% for high flows in an extrapolated section of the rating curve at the Mahurangi River (New Zealand) and 28 to 101% for low flows at the Taf River (United Kingdom), where the hydraulic control is unstable at low flows. Differences between methods result from differences in the sources of uncertainty considered, differences in the handling of the time‐varying nature of rating curves, differences in the extent of hydraulic knowledge assumed, and differences in assumptions when extrapolating rating curves above or below the observed gaugings. Ultimately, the selection of an uncertainty method requires a match between user requirements and the assumptions made by the uncertainty method. Given the significant differences in uncertainty estimates between methods, we suggest that a clear statement of uncertainty assumptions be presented alongside streamflow uncertainty estimates.
Tidal Response of Groundwater in a Leaky Aquifer—Application to Oklahoma
Authors: Wang Chi‐Yuen , Doan Mai‐Linh , Xue Lian , Barbour Andrew J. 
Published: August 2018

AbstractQuantitative interpretation of the tidal response of water levels measured in wells has long been made either with a model for perfectly confined aquifers or with a model for purely unconfined aquifers. However, many aquifers may be neither totally confined nor purely unconfined at the frequencies of tidal loading but behave somewhere between the two end‐members. Here we present a more general model for the tidal response of groundwater in aquifers with both horizontal flow and vertical leakage. The model has three independent parameters: the transmissivity (T) and storativity (S) of the aquifer and the specific leakage (K′/b′) of the leaking aquitard, where K′ and b′ are the hydraulic conductivity and the thickness of the aquitard, respectively. If T and S are known independently, this model may be used to estimate aquitard leakage from the phase shift and amplitude ratio of water level in wells obtained from tidal analysis. We apply the model to interpret the tidal response of water level in a US Geological Survey (USGS) deep monitoring well installed in the Arbuckle aquifer in Oklahoma, into which massive amount of wastewater coproduced from hydrocarbon exploration has been injected. The analysis shows that the Arbuckle aquifer is leaking significantly at this site. We suggest that the present method may be effective and economical for monitoring leakage in groundwater systems, which bears on the safety of water resources, the security of underground waste repositories, and the outflow of wastewater during deep injection and hydrocarbon extraction.
ΔNp63 regulates the expression of hyaluronic acid-related genes in breast cancer cells
Authors: Gatti Veronica , Fierro Claudia , Compagnone Mirco , Giangrazi Federica , Markert Elke Katrin , Bongiorno-Borbone Lucilla , Melino Gerry , Peschiaroli Angelo 
Published: August 2018

AbstractTriple negative breast cancers (TNBC) represent the most aggressive and clinically relevant breast carcinomas. On the basis of specific molecular signature, the majority of TNBC can be classified as basal-like breast carcinoma. Here, we report data showing that in basal-like breast carcinoma cells ΔNp63 is capable of sustaining the production of the hyaluronic acid (HA), one of the major component of the extracellular matrix (ECM). At molecular level, we found that ΔNp63 regulates the expression of HA-related genes, such as the HA synthase HAS3, the hyaluronidase HYAL-1 and CD44, the major HA cell membrane receptor. By controlling this pathway, ∆Np63 contributes to maintain the self-renewal of breast cancer stem cells. Importantly, high HAS3 expression is a negative prognostic factor of TNBC patients. Our data suggest that in basal-type breast carcinoma ∆Np63 might favor a HA-rich microenviroment, which can sustain tumor proliferation and stemness.
MAP1B mutations cause intellectual disability and extensive white matter deficit
Authors: Walters G. Bragi , Gustafsson Omar , Sveinbjornsson Gardar , Eiriksdottir Valgerdur K. , Agustsdottir Arna B. , Jonsdottir Gudrun A. , Steinberg Stacy , Gunnarsson Arni F. , Magnusson Magnus I. , Unnsteinsdottir Unnur , Lee Amy L. , Jonasdottir Adalbjorg , Sigurdsson Asgeir , Jonasdottir Aslaug , Skuladottir Astros , Jonsson Lina , Nawaz Muhammad S. , Sulem Patrick , Frigge Mike , Ingason Andres , Love Askell , Norddhal Gudmundur L. , Zervas Mark , Gudbjartsson Daniel F. , Ulfarsson Magnus O. , Saemundsen Evald , Stefansson Hreinn , Stefansson Kari 
Published: August 2018

AbstractDiscovery of coding variants in genes that confer risk of neurodevelopmental disorders is an important step towards understanding the pathophysiology of these disorders. Whole-genome sequencing of 31,463 Icelanders uncovers a frameshift variant (E712KfsTer10) in microtubule-associated protein 1B (MAP1B) that associates with ID/low IQ in a large pedigree (genome-wide corrected P = 0.022). Additional stop-gain variants in MAP1B (E1032Ter and R1664Ter) validate the association with ID and IQ. Carriers have 24% less white matter (WM) volume (β = −2.1SD, P = 5.1 × 10−8), 47% less corpus callosum (CC) volume (β = −2.4SD, P = 5.5 × 10−10) and lower brain-wide fractional anisotropy (P = 6.7 × 10−4). In summary, we show that loss of MAP1B function affects general cognitive ability through a profound, brain-wide WM deficit with likely disordered or compromised axons.
Black carbon radiative effects highly sensitive to emitted particle size when resolving mixing-state diversity
Authors: Matsui Hitoshi , Hamilton Douglas S. , Mahowald Natalie M. 
Published: August 2018

AbstractPost-industrial increases in atmospheric black carbon (BC) have a large but uncertain warming contribution to Earth’s climate. Particle size and mixing state determine the solar absorption efficiency of BC and also strongly influence how effectively BC is removed, but they have large uncertainties. Here we use a multiple-mixing-state global aerosol microphysics model and show that the sensitivity (range) of present-day BC direct radiative effect, due to current uncertainties in emission size distributions, is amplified 5–7 times (0.18–0.42 W m−2) when the diversity in BC mixing state is sufficiently resolved. This amplification is caused by the lifetime, core absorption, and absorption enhancement effects of BC, whose variability is underestimated by 45–70% in a single-mixing-state model representation. We demonstrate that reducing uncertainties in emission size distributions and how they change in the future, while also resolving modeled BC mixing state diversity, is now essential when evaluating BC radiative effects and the effectiveness of BC mitigation on future temperature changes.
Replication of bacterial plasmids in the nucleus of the red alga Porphyridium purpureum
Authors: Li Zhichao , Bock Ralph 
Published: August 2018

AbstractRhodophytes (red algae) are a diverse group of algae with great ecological and economic importance. However, tools for post-genomic research on red algae are still largely lacking. Here, we report the development of an efficient genetic transformation system for the model rhodophyte Porphyridium purpureum. We show that transgenes can be expressed to unprecedented levels of up to 5% of the total soluble protein. Surprisingly, the transgenic DNA is maintained episomally, as extrachromosomal high-copy number plasmid. The bacterial replication origin confers replication in the algal nucleus, thus providing an intriguing example of a prokaryotic replication origin functioning in a eukaryotic system. The extended presence of bacterial episomal elements may provide an evolutionary explanation for the frequent natural occurrence of extrachromosomal plasmids in red algae, and may also have contributed to the high rate of horizontal gene transfer from bacteria to the nuclear genome of Porphyridium purpureum and other rhodophytes.
Group I Paks are essential for epithelial- mesenchymal transition in an Apc-driven model of colorectal cancer
Authors: Chow H. Y. , Dong B. , Valencia C. A. , Zeng C. T. , Koch J. N. , Prudnikova T. Y. , Chernoff J. 
Published: August 2018

Abstractp21-activated kinases (Paks) play an important role in oncogenic signaling pathways and have been considered as potential therapeutic targets in various cancers. Most studies of Pak function employ gene knock-out or knock-down methods, but these approaches result in loss of both enzymatic and scaffolding properties of these proteins, and thus may not reflect the effects of small molecule inhibitors. Here we use a transgenic mouse model in which a specific peptide inhibitor of Group I Paks is conditionally expressed in response to Cre recombinase. Using this model, we show that inhibition of endogenous Paks impedes the transition of adenoma to carcinoma in an Apc-driven mouse model of colorectal cancer. These effects are mediated by inhibition of Wnt signaling through reduced β-catenin activity as well as suppression of an epithelial-mesenchymal transition program mediated by miR-200 and Snai1. These results highlight the potential therapeutic role of Pak1 inhibitors in colorectal cancer.
Transformation from crystalline precursor to perovskite in PbCl2-derived MAPbI3
Authors: Stone Kevin H. , Gold-Parker Aryeh , Pool Vanessa L. , Unger Eva L. , Bowring Andrea R. , McGehee Michael D. , Toney Michael F. , Tassone Christopher J. 
Published: August 2018

AbstractUnderstanding the formation chemistry of metal halide perovskites is key to optimizing processing conditions and realizing enhanced optoelectronic properties. Here, we reveal the structure of the crystalline precursor in the formation of methylammonium lead iodide (MAPbI3) from the single-step deposition of lead chloride and three equivalents of methylammonium iodide (PbCl2 + 3MAI) (MA = CH3NH3). The as-spun film consists of crystalline MA2PbI3Cl, which is composed of one-dimensional chains of lead halide octahedra, coexisting with disordered MACl. We show that the transformation of precursor into perovskite is not favored in the presence of MACl, and thus the gradual evaporation of MACl acts as a self-regulating mechanism to slow the conversion. We propose the stable precursor phase enables dense film coverage and the slow transformation may lead to improved crystal quality. This enhanced chemical understanding is paramount for the rational control of film deposition and the fabrication of superior optoelectronic devices.
Non-negligible Water-permeance through Nanoporous Ion Exchange Medium
Authors: Lee Jung A. , Lee Dokeun , Park Sungmin , Lee Hyomin , Kim Sung Jae 
Published: August 2018

AbstractWhile the water impermeable constraint has been conventionally adopted for analyzing the transport phenomena at the interface of electrolyte/nanoporous medium, non-negligible water-permeance through the medium results in significant effect on ion and particle transportation. In this work, a rigorous theoretical and experimental analysis of the water-permeance effect were conducted based on a fully-coupled analytical/numerical method and micro/nanofluidic experiments. The regime diagram with three distinctive types of concentration boundary layers (ion depletion, ion accumulation, and intermediate) near the ion exchange nanoporous medium was proposed depending on the medium’s permselectivity and the water-permeance represented by an absorbing parameter. Moreover, the critical absorbing parameters which divide the regimes were analytically obtained so that the bidirectional motion of particles were demonstrated only by altering the water-permeance without external stimuli. Conclusively, the presenting analysis of non-negligible water-permeance would be a substantial fundamental of transport phenomena at the interface of the ion exchange medium and electrolyte, especially useful for the tunable particle/ion manipulations in intermediate Peclet number environment.
Efavirenz exposure, alone and in combination with known drugs of abuse, engenders addictive-like bio-behavioural changes in rats
Authors: Möller Marisa , Fourie Jaco , Harvey Brian H. 
Published: August 2018

AbstractEfavirenz is abused in a cannabis-containing mixture known as Nyaope. The addictive-like effects of efavirenz (5, 10 and 20 mg/kg) was explored using conditioned place preference (CPP) in rats following sub-acute exposure vs. methamphetamine (MA; 1 mg/kg) and Δ9-tetrahydrocannabinol (THC; 0.75 mg/kg). The most addictive dose of efavirenz was then compared to THC alone and THC plus efavirenz following sub-chronic exposure using multiple behavioural measures, viz. CPP, sucrose preference test (SPT) and locomotor activity. Peripheral superoxide dismutase (SOD), regional brain lipid peroxidation and monoamines were also determined. Sub-acute efavirenz (5 mg/kg) had a significant rewarding effect in the CPP comparable to MA and THC. Sub-chronic efavirenz (5 mg/kg) and THC + efavirenz were equally rewarding using CPP, with increased cortico-striatal dopamine (DA), and increased lipid peroxidation and SOD. Sub-chronic THC did not produce CPP but significantly increased SOD and decreased hippocampal DA. Sub-chronic THC + efavirenz was hedonic in the SPT and superior to THC alone regarding cortico-striatal lipid peroxidation and sucrose preference. THC + efavirenz increased cortico-striatal DA and decreased serotonin (5-HT). Concluding, efavirenz has dose-dependent rewarding effects, increases oxidative stress and alters regional brain monoamines. Efavirenz is hedonic when combined with THC, highlighting its abuse potential when combined with THC.
Analysis of Bacterial Communities in Partial Nitritation and Conventional Nitrification Systems for Nitrogen Removal
Authors: Zhao Zhirui , Luo Jinxue , Jin Bo , Zhang Jiayao , Li Bin , Ma Bin , An Xiaoyu , Zhang Shujun , Shan Baoqing 
Published: August 2018

AbstractThis work studied the microbial community in partial nitritation and complete nitrification processes, which were applied to treat the low Carbon Nitrogen ratio wastewater. The phospholipid fatty acid and quantitative PCR analysis showed that the sludge circulating ratio of 75% resulted in a good microbial growth and a higher abundance of ammonia oxidizing bacteria relative to the nitrite oxidizing bacteria. The Betaproteobacteria were observed to compose the most abundant sludge bacterial groups in the two processes, based on phylogenetic analysis. The phylogenetic analysis of both 16S rRNA and amoA gene indicated that the Nitrosomonas sp. were the dominant ammonia oxidizing bacteria in the partial nitritation process. The relative abundance of nitrite oxidizing bacteria, such as Nitrobacter sp. and Nitrospira sp., were significantly lower in the partial nitritation system over the complete nitrification system. The abundance of Planctomycetes was higher in the partial nitritation process, indicating the anammox reaction occurred in the partial nitritation system. These results suggested the nitrite accumulation rate of circulating ratios 75% was the highest, with an average of 92%,and a possibility to treat the low Carbon Nitrogen ratio wastewater using the partial nitritation/anammox process.
Frequent patient retraining at home reduces the risks of peritoneal dialysis-related infections: A randomised study
Authors: Chang Jae Hyun , Oh Jieun , Park Sue K. , Lee Juyeon , Kim Sung Gyun , Kim Soo Jin , Shin Dong Ho , Hwang Young-Hwan , Chung Wookyung , Kim Hyunwook , Oh Kook-Hwan 
Published: August 2018

AbstractThe present study, entitled Trial on Education And Clinical outcomes for Home PD patients (TEACH), investigated the effect of frequent retraining at home on the outcomes of peritoneal dialysis (PD). TEACH is a multicentre, open-label, randomised, controlled trial with parallel arms. Patients starting PD were randomized into either the conventional retraining group (CG) or the frequent retraining group (FG). Patients in the FG were given more frequent home visits for retraining. The primary endpoint was exit site infection (ESI). Secondary endpoints were peritonitis, any PD-related infections, hospitalization, technique failure, and patient survival. A generalised estimating equations (GEE) approach was employed for the adjusted effect of training level on the outcomes. Cox regression was employed for peritonitis and other secondary outcomes. The subjects were randomised to either the FG (n = 51) or the CG (n = 53). Although the time of initial training did not differ between the 2 groups, the total time of training was longer and the frequency of training visits was higher in the FG. In the GEE model, the p-values for interactions between groups and time were significant for both ESI and any PD-related infections, suggesting that the event rates of the two groups significantly changed over time. The event rates for the FG decreased over time, and the event rates for the CG increased after month 12. In the older subgroup (age ≥ 60), frequent retraining had a significant effect in the risk reduction of the first episode of peritonitis (adjusted HR 0.01 [0.001–0.35], p = 0.01). Frequent retraining at home reduced the risk of PD-related infections.
Quadruple Semitendinosus Graft Construct With Double Cortical Suspensory Fixation for Anterior Cruciate Ligament Reconstruction: A Biomechanical Study
Authors: Madaíl Carla Alexandra , Vaz Maria de Fátima , Amaral Pedro Miguel , Consciência José Guimarães , Silva Alcindo Lucas 
Published: August 2018

AbstractThe purpose of this study was to evaluate the biomechanical properties of a graft construct with quadrupled Semitendinosus and two cortical buttons with adjustable loops concerning elongation, stiffness and resistance. A total of 15 fresh human cadaveric semitendinosus tendons were quadrupled over the two adjustable loops and stitched at the tibial tip with a cerclage type suture. They underwent pre-tensioning at 300 N for 2 minutes followed by cyclic loading (1000 cycles between 50–250 N) and finally a load-to-failure test. Statistical analysis was performed using SPSS Statistics software and groups were compared using a paired t-test, with a significance level set at α = 0.05. Graft construct elongation after pre-tensioning at 300 N was 12.8 mm (9.3 mm–16.5 mm) and mean cyclic elongation 0.4 mm (0.2 mm–0.9 mm), considered significant (p < 0,001). The resistance and stiffness values were respectively 849.46 N (649.30 N-1027.90 N) and 221.49 N (178,30 N – 276.10 N). Quadruple ST graft construct using two cortical buttons and adjustable loops showed a high stiffness and resistance with a very low elongation after cycling.
Optogenetic induction of the schizophrenia-related endophenotype of ventral hippocampal hyperactivity causes rodent correlates of positive and cognitive symptoms
Authors: Wolff Amy R. , Bygrave Alexei M. , Sanderson David J. , Boyden Edward S. , Bannerman David M. , Kullmann Dimitri M. , Kätzel Dennis 
Published: August 2018

AbstractPathological over-activity of the CA1 subfield of the human anterior hippocampus has been identified as a potential predictive marker for transition from a prodromal state to overt schizophrenia. Psychosis, in turn, is associated with elevated activity in the anterior subiculum, the hippocampal output stage directly activated by CA1. Over-activity in these subfields may represent a useful endophenotype to guide translationally predictive preclinical models. To recreate this endophenotype and study its causal relation to deficits in the positive and cognitive symptom domains, we optogenetically activated excitatory neurons of the ventral hippocampus (vHPC; analogous to the human anterior hippocampus), targeting the ventral subiculum. Consistent with previous studies, we found that vHPC over-activity evokes hyperlocomotion, a rodent correlate of positive symptoms. vHPC activation also impaired performance on the spatial novelty preference (SNP) test of short-term memory, regardless of whether stimulation was applied during the encoding or retrieval stage of the task. Increasing dopamine transmission with amphetamine produced hyperlocomotion, but was not associated with SNP impairments. This suggests that short-term memory impairments resulting from hippocampal over-activity likely arise independently of a hyperdopaminergic state, a finding that is consistent with the pharmaco-resistance of cognitive symptoms in patients.