bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptional landscape of soybean (Glycine max) embryonic axes during germination in the presence of paclobutrazol, a gibberellin biosynthesis inhibitor
Authors: Gazara Rajesh K. , de Oliveira Eduardo A. G. , A. Oliveira Antônia Elenir , Venancio Thiago M. 
Published: November 2018

ABSTRACTGibberellins (GA) are key positive regulators of seed germination. Although the GA effects on seed germination have been studied in a number of species, little is known about the transcriptional reprogramming modulated by GA during this phase in species other than Arabidopsis thaliana. Here we report the transcriptome analysis of soybean embryonic axes during germination in the presence of paclobutrazol (PBZ), a GA biosynthesis inhibitor. We found a number of differentially expressed cell wall metabolism genes, supporting their roles in cell expansion during germination. Several genes involved in the biosynthesis and signaling of other phytohormones were also modulated, indicating an intensive hormonal crosstalk at the embryonic axis. We have also found 26 photosynthesis genes that are up-regulated by PBZ at 24 hours of imbibition (HAI) and down-regulated at 36 HAI, which led us to suggest that this is part of a strategy to implement an autotrophic growth program in the absence of GA-driven mobilization of reserves. Finally, 30 transcription factors (mostly from the MYB, bHLH and bZIP families) that are down-regulated by PBZ and are likely downstream GA targets that will drive transcriptional changes during germination.
A macaque connectome for large-scale network simulations in TheVirtualBrain
Authors: Shen Kelly , Bezgin Gleb , Schirner Michael , Ritter Petra , Everling Stefan , McIntosh Anthony R. 
Published: November 2018

AbstractModels of large-scale brain networks that are informed by the underlying anatomical connectivity contribute to our understanding of the mapping between the structure of the brain and its dynamical function. Connectome-based modelling is a promising approach to a more comprehensive understanding of brain function across spatial and temporal scales, but it must be constrained by multi-scale empirical data from animal models. Here we describe the construction of a macaque connectome for whole-cortex simulations in TheVirtualBrain, an open-source simulation platform. We take advantage of available axonal tract-tracing datasets and enhance the existing connectome data using diffusion-based tractography in macaques. We illustrate the utility of the connectome as an extension of TheVirtualBrain by simulating resting-state BOLD-fMRI data and fitting it to empirical resting-state data.
E-ChRPs: Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning
Authors: Donovan DA , Crandall JG , Banks OGB , Jensvold ZD , McKnight LE , McKnight JN 
Published: November 2018

SummaryRegulation of chromatin structure is essential for controlling the access of DNA to factors that require association with specific DNA sequences. The ability to alter chromatin organization in a targeted manner would provide a mechanism for directly manipulating DNA-dependent processes and should provide a means to study direct consequences of chromatin structural changes. Here we describe the development and validation of engineered chromatin remodeling proteins (E-ChRPs) for inducing programmable changes in nucleosome positioning by design. We demonstrate that E-ChRPs function both in vivo and in vitro to specifically reposition target nucleosomes and entire nucleosomal arrays, and possess the ability to evict native DNA-binding proteins through their action. E-ChRPs can be designed with a range of targeting modalities, including the SpyCatcher and dCas9 moieties, resulting in high versatility and enabling diverse future applications. Thus, engineered chromatin remodeling proteins represent a simple and robust means to probe regulation of DNA-dependent processes in different chromatin contexts.
The modular mechanism of chromocenter formation in Drosophila
Authors: Jagannathan Madhav , Cummings Ryan , Yamashita Yukiko M. 
Published: November 2018

AbstractA central principle underlying the ubiquity and abundance of pericentromeric satellite DNA repeats in eukaryotes has remained poorly understood. In our previous study (Jagannathan et al., 2018), we proposed that the interchromosomal clustering of satellite DNAs into nuclear structures known as chromocenters ensures encapsulation of all chromosomes into a single nucleus. Chromocenter disruption led to micronuclei formation, resulting in cell death. Here we show that chromocenter formation is mediated by a ‘modular’ network, where interactions between two sequence-specific satellite DNA-binding proteins, D1 and Prod, bound to their cognate satellite DNAs, bring the full complement of chromosomes into the chromocenter. D1 prod double mutants die during embryogenesis, exhibiting enhanced phenotypes associated with chromocenter disruption, revealing the universal importance of satellite DNAs and chromocenters. Taken together, we propose that interactions between chromocenter modules, consisting of satellite DNA binding proteins and their cognate satellite DNA, package the Drosophila genome within a single nucleus.
Factors Associated with Risk Of HIV-Infection Among Pregnant Women in Cameroon: Evidence from the 2016 National Sentinel Surveillance Survey of HIV and syphilis
Authors: Anoubissi Jean de Dieu , Gabriel Ekali Loni , Nde Cyprien Kengne , Fokam Joseph , Tseuko Dorine Godelive , Messeh Arlette , Moussa Yasmine , Nkenfou Celine Nguefeu , Bonono Leonard , Billong Serge-Clotaire , Nfetam Jean-Bosco Elat 
Published: November 2018

AbstractBackgroundHuman Immunodeficiency Virus infection (HIV) remains a public health concern in Cameroon that requires regular surveillance for informed policy-making to guide programmatic interventions. Using data from the 2016 HIV national sentinel survey in Cameroon, we ascertained HIV prevalence and factors associated with risk of infection among pregnant womenMethodsA cross-sectional study was conducted throughout 2016 in the 10 regions of Cameroon, targeting 7000 first antenatal care (ANC-1) attendees (4000 from urban and 3000 from rural areas) in 60 sentinel health facilities. HIV serological test was performed using the national serial algorithm at the National Reference Laboratory (NRL). Prevalence was determined, and multivariate logistic regression was used to assess determinants of HIV infection, with p-value<0.05 considered statistically significant.ResultsOf the 7000 targeted participants, a total of 6859 first ANC-1 attendees were enrolled (98.0% sampling coverage). Median age was 26 [IQR: 21-30] years and 47,40% had a secondary school level of education. The national prevalence of HIV was 5.70% (95% CI: 4.93 – 6.40) and range from 9.7% in East region to 2.6% in North region. The prevalence was 5.58% (95% CI: 95%: 4.88 – 6.35) in urban and 5.87% (95% CI: 5.04 – 6.78) in rural settings. Factors that were associated with HIV infection included marital status, women who were married or living with their partner are less likely to be infected than singles women (aOR=0.60; 95% CI: 0.46 – 0.78), multiparity [aOR=1.5(95%CI:1.0-2.2)] and been living in the Centre, East, North-west and South-west regions. HIV infection was also significantly associated with age, with the risk of being infected increasing with age.ConclusionPregnant women in Cameroon are still disproportionately infected with HIV compared with the general population (prevalence 4.3%). Preventive actions to curb the epidemic amongst pregnant women should prioritize interventions targeting single pregnant women, who are older, and residing particularly in the Centre, East, North West and South West regions of the country.
A critical role of the T3SS effector EseJ in intracellular trafficking and replication ofEdwardsiella piscicidain non-phagocytic cells
Authors: Zhang Lingzhi , Jiang Jiatiao , Hu Tianjian , Zhang Jin , Liu Xiaohong , Yang Dahai , Zhang Yuanxing , Liu Qin 
Published: November 2018

AbstractEdwardsiella piscicida(E. piscicida) is an intracellular pathogen within a broad spectrum of hosts. Essential toE. piscicidavirulence is its ability to survive and replicate inside host cells, yet the underlying mechanisms and the nature of the replicative compartment remain unclear. Here, we characterized its intracellular lifestyle in non-phagocytic cells and showed that intracellular replication ofE. piscicidain non-phagocytic cells is dependent on its type III secretion system. Following internalization,E. piscicidais contained in vacuoles that transiently mature into early endosomes, but subsequently bypasses the classical endosome pathway and fusion with lysosomes which depends on its T3SS. Following a rapid escape from the degradative pathway,E. piscicidawas found to create a specialized replication-permissive niche characterized by endoplasmic reticulum (ER) markers. We also found that a T3SS effector EseJ is responsible for intracellular replication ofE. piscicidaby preventing endosome/lysosome fusion. Furthermore,in vivoexperiments confirmed that EseJ is necessary for bacterial colonization ofE. piscicidain both mice and zebrafish. Thus, this work elucidates the strategies used byE. piscicidato survive and proliferate within host non-phagocytic cells.Author summaryE. piscicidais a facultative intracellular bacterium associated with septicemia and fatal infections in many animals, including fish and humans. However, little is known about its intracellular life, which is important for successful invasion of the host. The present study is the first comprehensive characterization ofE. piscicida’s intracellular life-style in host cells. Upon internalization,E. piscicidais transiently contained in Rab5-positive vacuoles, but the pathogen prevents further endosome maturation and fusion with lysosomes by utilizing an T3SS effector EseJ. In addition, the bacterium creates an specialized replication niche for rapid growth via an interaction with the ER. Our study provides new insights into the strategies used byE. piscicidato successfully establishes an intracellular lifestyle that contributes to its survival and dissemination during infection.
Leveraging interactive social media communication for organizational success
Authors: Chen Feifei 
Published: November 2018

Purpose
The purpose of this paper is to examine how microblog communication enabled a new form of hybrid net-roots third-sector organization that rely heavily on the internet to achieve multiple organizational successes in civil society, social movement and service providing in China, where the government holds predominating power over the third sector.

Design/methodology/approach
A qualitative inductive analysis was conducted to analyze two successful organizations’ Sina tweets sent from their Weibo debuts to the dates when they achieved their first milestone successes. In the analysis, the author iteratively alternated between emic data coding and etic reference to literature on social movement rhetoric and nonprofits’ microblog communication.

Findings
This study developed an indigenous communication framework featuring three key communication strategies: changing perceptions, mobilizing action, and building and maintaining relationships, each associated with specific tactics. These strategies and tactics allowed both organizations to tap into social media’s interactive features to engage publics and construct legitimacy.

Research limitations/implications
This paper enriches social media-based communication research and classic social movement rhetoric, and further illustrates strategic communication’s active role in reacting to and reforming institutional contexts. Findings from study might be extended to address similar problems experienced by nonprofits across countries, especially within those that operate in a context where institutional separation from a predominant government is unavailable.

Originality/value
This original communication framework developed in this study crystalizes strategic microblog use by a nascent type of nonprofit when fulfilling functions reflects civil society, social movements and traditional nonprofit organizations in an understudied political and social context.
Top government hands-on megaproject management: the case of Istanbul’s grand airport
Authors: Eren Fatih 
Published: November 2018

Purpose
The purpose of this paper is to explore top government hands-on megaproject management model applied to Istanbul’s new airport megaproject. The study aims to find an answer to this research question: “How is the organizational design and management of a megaproject that has been alive in a confrontational socio-political environment and whose national strategic importance is high?”

Design/methodology/approach
Organizational theory and critical theory perspectives are used in this study. Istanbul’s grand airport is chosen as the case study. Interview, focus group and document analysis methods are used together. The research yields data from qualitative resources. Verbal and written materials are organized into three major themes and 27 key subjects for content analysis.

Findings
A top level of organizational management can be attained in the state in order for the mega projects with high national strategic importance to survive without problems and with high performance. The top government-level organizational structure implemented at Istanbul’s new airport has reduced complexity, risk and uncertainty; increased performance and product quality; and strenghtened inter-organizational compatibility and communication within the megaproject organization. However, this structuring also increased centralization, reduced transparency and direct public participation in the decision-making process.

Research limitations/implications
The megaproject is run in strict confidence and limited information is shared gradually in a controlled way with public by the megaproject organization. Therefore, a qualitative methodology is followed and the usage of quantitative data remained limited in the research.

Practical implications
The study presents an exemplary top government-level megaproject management model for countries experiencing strong socio-political conflicts and aiming to perform megaprojects with high national strategic importance.

Social implications
The organization of Istanbul’s new airport megaproject offers an exemplary, flexible and innovative organizational management model for countries that want to realize mega projects with high national strategic importance and experience strong socio-political conflicts. The questions of how to cope with challenges, how to build management capabilities and how to improve the cooperation and coordination within megaprojects have found some answers with this study. In addition, this study provides an insight into how to make more effective objections to wrong megaproject practices and the right strategies that the opposing organizations can follow.

Originality/value
This empirical research widens and deepens the theoretical foundations of top government hands-on megaproject management. The study, which includes a process analysis, allows to better understand the philosophy, nature, success, planning process, social organization and dynamics of megaprojects and to explain them through the values and strategies of organizations.
A good company gone bad
Authors: Park Young Eun , Son Hyunsang , Yang Sung-Un , Lee Jae Kook 
Published: November 2018

PurposeThe purpose of this paper is to demonstrate whether or not public relations efforts in corporate social responsibility (CSR) influence the news media in corporate crisis situations.Design/methodology/approachThe study conducted a content analysis of press releases and news media based on traditional human-coded cross-lag analyses and a machine learning technique, a novel method of big data analysis to test hypotheses.FindingsResults indicate that CSR press releases indeed influenced the news media. During the crisis point, however, agenda-building was not observed.Practical implicationsCorporations need to continue CSR activities and provide public relations materials consistently even after a crisis, as an agenda-building role could be recovered.Originality/valueThe study examines the relationship between CSR and crisis situations in an agenda-building theoretical framework. The authors introduce agenda-building in the corporate sector with machine learning techniques.
Social return on investment for community-based alcohol consumption control program during Buddhist Lent
Authors: Jirarattanasopha Varangkanar , Witvorapong Nopphol , Hanvoravongchai Piya 
Published: November 2018

Purpose
The purpose of this paper is to evaluate the cost and benefit of a community-based alcohol consumption control program during the Buddhist Lent (BL) period in terms of social return on investment (SROI).

Design/methodology/approach
The research team evaluated the program in four selected villages from four regions using standard SROI. Relevant stakeholders were involved in the evaluation design and program impact map construction. Data, including costs, were collected from literatures, official documents, stakeholder interviews and focus group discussions. Alcohol abstinence and related data during and after the 2015 BL period were gathered from a survey questionnaire. The SROI ratio presented the social benefits compared against the total social investment.

Findings
The program was effective in producing a greater social value (2.7–5.9 times) than the cost of investment in every village. Cost savings from alcohol consumption constituted a major proportion of the program’s value.

Originality/value
The community-based alcohol consumption control program during BL can provide value for investment. Information from this study can be used by policy makers in their decision to continue or scale up the program. The SROI approach mainly relies on stakeholders that may present a bias; however, further study such as social cost-benefit analysis could provide additional insights.
PLS path modeling – a confirmatory approach to study tourism technology and tourist behavior
Authors: Müller Tobias , Schuberth Florian , Henseler Jörg 
Published: November 2018

PurposeAs technology in tourism and hospitality (TTH) develops technical artifacts according to visitors’ demands, it must deal with both behavioral and design constructs in the context of structural equation modeling (SEM). While behavioral constructs are typically modeled as common factors, the study at hand introduces the composite into TTH to model artifacts. To deal with both kinds of constructs, this paper aims to exploit partial least squares path modeling (PLS-PM) as a confirmatory approach to estimate models containing common factors and composites.Design/methodology/approachThe study at hand presents PLS-PM in its current form, i.e. as a full-fledged approach for confirmatory purposes. By introducing the composite to model artifacts, TTH scholars can use PLS-PM to answer research questions of the type “Is artifact xyz useful?”, contributing to a further understanding of TTH. To demonstrate the composite model, an empirical example is used.FindingsPLS-PM is a promising approach when the model contains both common factors and composites. By applying the test for overall model fit, empirical evidence can be obtained for latent variables and artifacts. In doing so, researchers can statistically test whether a developed artifact is useful.Originality/valueTo the best of the authors’ knowledge, this is the first study to discuss the practical application of composite and common factor models in TTH research. Besides introducing the composite to model artifacts, the study at hand also guides scholars in the assessment of PLS-PM results.
Conceptualizing “project resiliency”
Authors: Holdsworth Laura M. 
Published: November 2018

Purpose
To meet the multidimensional needs of patients, health services are increasingly implementing complex programmes of care through partnerships between public, private and voluntary sector organisations. The purpose of this paper is to explore the implementation process of a complex, multi-innovative regional health and social care partnership to coordinate end-of-life care in the South East of England.

Design/methodology/approach
The study adopted a pragmatic, pluralist design using primarily qualitative methods including observations, interviews, focus group and document review. Implementation theory provided the research framework.

Findings
While progress was made towards greater collaboration in the provision of end-of-life care, regional coordination of care among the 13 partner organisations was not achieved as envisioned. Low engagement stemming from national health system changes delayed decision making and shifted partners’ priorities. Individual stakeholder interest and motivation carried the elements that were successful.

Practical implications
The external political and economic environment hindered the involvement of some of the partners and suggests that a concept of “project resiliency” is particularly important for complex, multi-organisational projects which are implemented over time and by multiple stakeholders from different sectors. Future research should look further at what contributes to project resiliency and whether it might be operationalized so that projects can develop resilient factors for success.

Originality/value
Project resiliency is a new concept that bridges a gap in understanding how time-limited multi-organisational projects function amid a changing environment.
Classifying the integrated public safety areas (IPSAs): a multi-criteria based approach
Authors: Basilio Marcio Pereira , Pereira Valdecy , Costa Helder Gomes 
Published: November 2018

Purpose
This study aims to focus on the application of a multiple criteria decision analysis (MCDA) method to compare results presented by the Integrated Goals System based on the 12th edition of the Integrated Public Safety Areas (IPSAs) Award, which achieved goals established for Strategic Crime Indicators for the state of Rio de Janeiro, Brazil. The main objective of this research was to limit the compensatory effects of classification criteria on IPSAs that have achieved goals established for crime indicators by applying the MCDA method.

Design/methodology/approach
The methodology was based on the application of MCDA. The MCDA method selected and used was ELECTRE III, being implemented by J-Electre software.

Findings
Compared to results of the current method, the results of the ELECTRE III method showed a 94.87 per cent change in ranking positions revealed via the SIM method. This finding denotes the elimination of compensatory effects of the criteria. As a consequence, it can be affirmed that the application of resources by IPSA managers to reduce the prevalence of a single strategic crime indicator is no longer a success factor for awarding in the established goal system.

Research limitations/implications
As limitations, it is possible to indicate the time cut used to carry out the research. The research may be extended to other issues of the productivity award.

Practical implications
The methodology applied with the use of ELECTRE III revealed that the government could reduce the cost with the incentive program to reduce criminal indices.

Social implications
As the MCDA method is based on a binary pairwise comparison system, the methodology imposes a change of attitude on local managers fighting crime to reduce crime indicators and to consequently build a local sense of safety in IPSAs.

Originality/value
This research fills a gap in the literature because there are few studies using the MCDA method in the field of public security. The value of the work lies in the creation of a method that gives the decision-maker, of the law enforcement agency, an alternative to improve the process of rewarding by productividad of the integrated areas of public security.