bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Question of the Examined Life
Authors: Gillespie Michael Allen 
Published: April 2018

AbstractThis essay calls into question Zuckert's claim in Postmodern Platos that Strauss provides the best contemporary defense of the superiority of the philosophic life against the claims of Nietzsche and Heidegger that it leads to nihilism and despair. For her, Strauss persuasively draws on Plato, read through Alfarabi and Maimonides, to defend this view by showing that the philosopher understands the true ends of human life as a whole which is part of the whole, and thus provides a vision of the noblest, best, and most beautiful. I argue that this claim is implausible, that Strauss's Platonic vision of the ends of human life is obscure, and that even if correct, it does not offer an answer to the question of the relative value of these heterogenous ends, and thus does not demonstrate that the philosophic life is more worth living than any other form of life.
CONSTITUTIONS, RULE FOLLOWING, AND THE CRISIS OF CONSTRAINT
Authors: Crocker Thomas P. , Hodges Michael P. 
Published: April 2018

ABSTRACTWe diagnose a paradox said to exist for liberal constitutionalism. Constitutional rules seemed to fail to constrain in times of expediency or under conditions of necessity, occasions when constraints might be most needed. From this failure, a form of rule skepticism seems to undermine liberal constitutionalism's claims to govern and constrain official behavior, especially during times of emergency. On closer inspection, we identify three different forms this purported rule skepticism might take: contestation over rule applications, skepticism about rule determinations, or rule cynicism. We resolve the apparent paradox by demonstrating how rule skepticism is either unwarranted or cynically deployed, but in neither case raises a legitimate challenge to liberal constitutionalism.
The Global Food‐Energy‐Water Nexus
Authors: D'Odorico Paolo , Davis Kyle Frankel , Rosa Lorenzo , Carr Joel A. , Chiarelli Davide , Dell'Angelo Jampel , Gephart Jessica , MacDonald Graham K. , Seekell David A. , Suweis Samir , Rulli Maria Cristina 
Published: April 2018

AbstractWater availability is a major factor constraining humanity's ability to meet the future food and energy needs of a growing and increasingly affluent human population. Water plays an important role in the production of energy, including renewable energy sources and the extraction of unconventional fossil fuels that are expected to become important players in future energy security. The emergent competition for water between the food and energy systems is increasingly recognized in the concept of the “food‐energy‐water nexus.” The nexus between food and water is made even more complex by the globalization of agriculture and rapid growth in food trade, which results in a massive virtual transfer of water among regions and plays an important role in the food and water security of some regions. This review explores multiple components of the food‐energy‐water nexus and highlights possible approaches that could be used to meet food and energy security with the limited renewable water resources of the planet. Despite clear tensions inherent in meeting the growing and changing demand for food and energy in the 21st century, the inherent linkages among food, water, and energy systems can offer an opportunity for synergistic strategies aimed at resilient food, water, and energy security, such as the circular economy.
International Collaboration Within the United Nations Committee on the Peaceful Uses of Outer Space: Framework for International Space Weather Services (2018–2030)
Authors: Mann I. R. , Di Pippo S. , Opgenoorth H. J. , Kuznetsova M. , Kendall D. J. 
Published: April 2018

AbstractSevere space weather is a global threat that requires a coordinated global response. In this Commentary, we review some previous successful actions supporting international coordination between member states in the United Nations (UN) context and make recommendations for a future approach. Member states of the UN Committee on the Peaceful Uses of Outer Space (COPUOS) recently approved new guidelines related to space weather under actions for the long‐term sustainability of outer space activities. This is to be followed by UN Conference on the Exploration and Peaceful Uses of Outer Space (UNISPACE)+50, which will take place in June 2018 on the occasion of the fiftieth anniversary of the first UNISPACE I held in Vienna in 1968. Expanded international coordination has been proposed within COPUOS under the UNISPACE+50 process, where priorities for 2018–2030 are to be defined under Thematic Priority 4: Framework for International Space Weather Services. The COPUOS expert group for space weather has proposed the creation of a new International Coordination Group for Space Weather be implemented as part of this thematic priority. This coordination group would lead international coordination between member states and across international stakeholders, monitor progress against implementation of guidelines and best practices, and promote coordinated global efforts in the space weather ecosystem spanning observations, research, modeling, and validation, with the goal of improved space weather services. We argue that such improved coordination at the international policy level is essential for increasing global resiliency against the threats arising from severe space weather.
Optically sizing single atmospheric particulates with a 10-nm resolution using a strong evanescent field
Authors: Yu Xiao-Chong , Zhi Yanyan , Tang Shui-Jing , Li Bei-Bei , Gong Qihuang , Qiu Cheng-Wei , Xiao Yun-Feng 
Published: April 2018

AbstractAlthough an accurate evaluation of the distribution of ultrafine particulate matter in air is of utmost significance to public health, the usually used PM2.5 index fails to provide size distribution information. Here we demonstrate a low-profile and cavity-free size spectrometer for probing fine and ultrafine particulate matter by using the enhanced particle-perturbed scattering in strong optical evanescent fields of a nanofiber array. The unprecedented size resolution reaches 10 nm for detecting single 100-nm-diameter nanoparticles by employing uniform nanofibers and controlling the polarizations of the probe light. This size spectrometry was tested and used to retrieve the size distribution of particulate matter in the air of Beijing, yielding mass concentrations of nanoparticles, as a secondary exercise, consistent with the officially released data. This nanofiber-array probe shows potential for the full monitoring of air pollution and for studying early-stage haze evolution and can be further extended to explore nanoparticle interactions.
Therapeutic endocannabinoid augmentation for mood and anxiety disorders: comparative profiling of FAAH, MAGL and dual inhibitors
Authors: Bedse Gaurav , Bluett Rebecca J. , Patrick Toni A. , Romness Nicole K. , Gaulden Andrew D. , Kingsley Philip J. , Plath Niels , Marnett Lawrence J. , Patel Sachin 
Published: April 2018

AbstractRecent studies have demonstrated anxiolytic potential of pharmacological endocannabinoid (eCB) augmentation approaches in a variety of preclinical models. Pharmacological inhibition of endocannabinoid-degrading enzymes, such as fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL), elicit promising anxiolytic effects in rodent models with limited adverse behavioral effects, however, the efficacy of dual FAAH/MAGL inhibition has not been investigated. In the present study, we compared the effects of FAAH (PF-3845), MAGL (JZL184) and dual FAAH/MAGL (JZL195) inhibitors on (1) anxiety-like behaviors under non-stressed and stressed conditions, (2) locomotor activity and body temperature, (3) lipid levels in the brain and (4) cognitive functions. Behavioral analysis showed that PF-3845 or JZL184, but not JZL195, was able to prevent restraint stress-induced anxiety in the light–dark box assay when administered before stress exposure. Moreover, JZL195 treatment was not able to reverse foot shock-induced anxiety-like behavior in the elevated zero maze or light–dark box. JZL195, but not PF-3845 or JZL184, decreased body temperature and increased anxiety-like behavior in the open-field test. Overall, JZL195 did not show anxiolytic efficacy and the effects of JZL184 were more robust than that of PF-3845 in the models examined. These results showed that increasing either endogenous AEA or 2-AG separately produces anti-anxiety effects under stressful conditions but the same effects are not obtained from simultaneously increasing both AEA and 2-AG.
Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model
Authors: Li Guigang , Zhang Yuan , Cai Subo , Sun Ming , Wang Juan , Li Shen , Li Xinyu , Tighe Sean , Chen Shuangling , Xie Huatao , Zhu Yingting 
Published: April 2018

AbstractIn this article, human limbal niche cells (LNC) or bone marrow derived mesenchymal stem cells (BMMSC) were used to prevent limbal stem cell deficiency (LSCD) in an alkali burn rabbit model and their results were compared. The epithelial cell defect area, corneal neovascularization, and the print cell cytometry were quantified to grade the severity of LSCD. Three months after the alkali burn, a partial LSCD was observed in the control group (no treatment) indicated by chronic corneal epithelial defects, positive corneal fluorescein staining, neovascularization and goblet cell migration. In contrast, the severity of LSCD in both the LNC and BMMSC transplantation groups was dramatically reduced as shown by smaller epithelial cell defects, decreased fluorescein sodium staining, decreased neovascularization and decreased goblet cell density. Interestingly, the LNC group was shown to more effectively prevent LSCD than the BMMSC group. Further analysis indicated subconjunctivally transplanted LNCs were more powerful than BMMSCs to prevent LSCD, at least partially, due to increased activation of SCF-c-Kit signal. We conclude that LNCs are a more powerful resource than BMMSCs to prevent LSCD in an alkali burn rabbit model, at least partially due to increased activation of SCF signaling.
Atomic optical stimulated amplifier with optical filtering of ultra-narrow bandwidth
Authors: Pan Duo , Shi Tiantian , Luo Bin , Chen Jingbiao , Guo Hong 
Published: April 2018

AbstractTaking advantages of ultra-narrow bandwidth and high noise rejection performance of the Faraday anomalous dispersion optical filter (FADOF), simultaneously with the coherent amplification of atomic stimulated emission, we propose a stimulated amplified Faraday anomalous dispersion optical filter (SAFADOF) at cesium 1470 nm. The SAFADOF is able to significantly amplify very weak laser signals and reject noise in order to obtain clean signals in strong background. We show that for a weak signal of 50 pW, the gain factor can be larger than 25000 (44 dB) within a bandwidth as narrow as 13 MHz. Having the ability to amplify weak signals with low background contribution, the SAFADOF finds outstanding potential applications in weak signal detections.
Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells
Authors: Lopata Anna , Hughes Ruth , Tiede Christian , Heissler Sarah M. , Sellers James R. , Knight Peter J. , Tomlinson Darren , Peckham Michelle 
Published: April 2018

AbstractImaging the actin cytoskeleton in cells uses a wide range of approaches. Typically, a fluorescent derivative of the small cyclic peptide phalloidin is used to image F-actin in fixed cells. Lifeact and F-tractin are popular for imaging the cytoskeleton in live cells. Here we characterised novel affinity reagents called Affimers that specifically bind to F-actin in vitro to determine if they are suitable alternatives as eGFP-fusion proteins, to label actin in live cells, or for labeling F-actin in fixed cells. In vitro experiments showed that 3 out of the 4 Affimers (Affimers 6, 14 and 24) tested bind tightly to purified F-actin, and appear to have overlapping binding sites. As eGFP-fusion proteins, the same 3 Affimers label F-actin in live cells. FRAP experiments suggest that eGFP-Affimer 6 behaves most similarly to F-tractin and Lifeact. However, it does not colocalise with mCherry-actin in dynamic ruffles, and may preferentially bind stable actin filaments. All 4 Affimers label F-actin in methanol fixed cells, while only Affimer 14 labels F-actin after paraformaldehyde fixation. eGFP-Affimer 6 has potential for use in selectively imaging the stable actin cytoskeleton in live cells, while all 4 Affimers are strong alternatives to phalloidin for labelling F-actin in fixed cells.
Optimized microwave extraction, characterization and antioxidant capacity of biological polysaccharides from Eucommia ulmoides Oliver leaf
Authors: Xu Jikun , Hou Huijie , Hu Jingping , Liu Bingchuan 
Published: April 2018

AbstractMicrowave-induced technique was combined with response surface methodology for optimizing the isolation of polysaccharides from Eucommia ulmoides Oliver leaf. The maximum polysaccharides yield of 12.31% was achieved by microwave extraction at 74 °C for 15 min with a solid to liquid ratio of 1:29 g/mL, which agreed with the predicted value and was 2.9-fold higher than that of the conventional heat-reflux extraction method. The dominant bioactive constituent in extracts was chlorogenic acid (1.3–1.9%), followed by geniposidic acid (1.0–1.7%). The polysaccharides from the optimized extraction had a high molecular weight and polydispersity (Mw 38,830 g/mol, Mw/Mn 2.19), as compared to the fraction prepared in the absence of microwave (Mw 12,055 g/mol, Mw/Mn 1.26). Glucose was the dominant sugar component (38.2–39.1%) of heterogeneous polysaccharides which belonged to a structure of β-type acidic heteropolysaccharides with a glucan group and highly branched degree. The polysaccharides showed a higher DPPH radical scavenging index (0.87–1.22) than BHT (0.41) but lower than BHA (3.56), which can act as a favorable antioxidant in functional food.
Multivalent exposure of trastuzumab on iron oxide nanoparticles improves antitumor potential and reduces resistance in HER2-positive breast cancer cells
Authors: Truffi Marta , Colombo Miriam , Sorrentino Luca , Pandolfi Laura , Mazzucchelli Serena , Pappalardo Francesco , Pacini Chiara , Allevi Raffaele , Bonizzi Arianna , Corsi Fabio , Prosperi Davide 
Published: April 2018

AbstractTargeted therapies have profoundly changed the clinical prospect in human epidermal growth factor receptor 2 (HER2)-positive breast cancer. In particular, the anti-HER2 monoclonal antibody trastuzumab represents the gold standard for the treatment of HER2+ breast cancer patients. Its contribution in dampening cancer progression is mainly attributed to the antibody-dependent cell-mediated cytotoxicity (ADCC) rather than HER2 blockade. Here, multiple half chains of trastuzumab were conjugated onto magnetic iron oxide nanoparticles (MNP-HC) to develop target-specific and biologically active nanosystems to enhance anti-HER2 therapeutic potential. HER2 targeting was assessed in different human breast cancer cell lines, where nanoparticles triggered site-specific phosphorylation in the catalytic domain of the receptor and cellular uptake by endocytosis. MNP-HC induced remarkable antiproliferative effect in HER2+ breast cancer cells, exhibiting enhanced activity compared to free drug. Accordingly, nanoparticles induced p27kip1 expression and cell cycle arrest in G1 phase, without loosing capability to prime ADCC. Finally, MNP-HC affected viability of trastuzumab-resistant cells, suggesting interference with the resistance machinery. Our findings indicate that multiple arrangement of trastuzumab half chain on the nanoparticle surface enhances anticancer efficacy in HER2+ breast cancer cells. Powerful inhibition of HER2 signaling could promote responsiveness of resistant cells, thus suggesting ways for drug sensitization.
Boosting Terahertz Photoconductive Antenna Performance with Optimised Plasmonic Nanostructures
Authors: Lepeshov Sergey , Gorodetsky Andrei , Krasnok Alexander , Toropov Nikita , Vartanyan Tigran A. , Belov Pavel , Alú Andrea , Rafailov Edik U. 
Published: April 2018

AbstractAdvanced nanophotonics penetrates into other areas of science and technology, ranging from applied physics to biology, which results in many fascinating cross-disciplinary applications. It has been recently demonstrated that suitably engineered light-matter interactions at the nanoscale can overcome the limitations of today’s terahertz (THz) photoconductive antennas, making them one step closer to many practical implications. Here, we push forward this concept by comprehensive numerical optimization and experimental investigation of a log-periodic THz photoconductive antenna coupled to a silver nanoantenna array. We shed light on the operation principles of the resulting hybrid THz antenna, providing an approach to boost its performance. By tailoring the size of silver nanoantennas and their arrangement, we obtain an enhancement of optical-to-THz conversion efficiency 2-fold larger compared with previously reported results for similar structures, and the strongest enhancement is around 1 THz, a frequency range barely achievable by other compact THz sources. We also propose a cost-effective fabrication procedure to realize such hybrid THz antennas with optimized plasmonic nanostructures via thermal dewetting process, which does not require any post processing and makes the proposed solution very attractive for applications.
Comparative analyses and structural insights of the novel cytochrome P450 fusion protein family CYP5619 in Oomycetes
Authors: Bamal Hans Denis , Chen Wanping , Mashele Samson Sitheni , Nelson David R. , Kappo Abidemi Paul , Mosa Rebamang Anthony , Yu Jae-Hyuk , Tuszynski Jack A. , Syed Khajamohiddin 
Published: April 2018

AbstractPhylogenetic and structural analysis of P450 proteins fused to peroxidase/dioxygenase has not been reported yet. We present phylogenetic and in silico structural analysis of the novel P450 fusion family CYP5619 from the deadliest fish pathogenic oomycete, Saprolegnia diclina. Data-mining and annotation of CYP5619 members revealed their unique presence in oomycetes. CYP5619 members have the highest number of conserved amino acids among eukaryotic P450s. The highest number of conserved amino acids (78%) occurred in the peroxidase/dioxygenase domain compared to the P450 domain (22%). In silico structural analysis using a high-quality CYP5619A1 model revealed that CYP5619A1 has characteristic P450 structural motifs including EXXR and CXG. However, the heme-binding domain (CXG) in CYP5619 members was found to be highly degenerated. The in silico substrate binding pattern revealed that CYP5619A1 have a high affinity to medium chain fatty acids. Interestingly, the controlling agent of S. diclina malachite green was predicted to have the highest binding affinity, along with linoleic acid. However, unlike fatty acids, none of the active site amino acids formed hydrogen bonds with malachite green. The study’s results will pave the way for assessing CYP5619A1’s role in S. diclina physiology, including the nature of malachite green binding.
Preservation of rat limbs by hyperbaric carbon monoxide and oxygen
Authors: Hatayama Naoyuki , Hirai Shuichi , Naito Munekazu , Terayama Hayato , Araki Jun , Yokota Hiroki , Matsushita Masayuki , Li Xiao-Kang , Itoh Masahiro 
Published: April 2018

AbstractCold ischemia times ranging from <6 h to as long as 24 h are generally quoted as the limits for attempting the replantation of amputated extremities. In this study, we aimed to assess the effect of hyperbaric carbon monoxide (CO) and oxygen (O2) on rat limb preservation. Donor rat limbs were preserved in a chamber filled with hyperbaric CO and O2 for 3 days (CO + O2 3 days) or 7 days (CO + O2 7 days). Positive and negative control groups were created by using non-preserved limbs (NP) and limbs wrapped in saline-moistened gauze for 3 days (SMG 3 days), respectively. The survival rate of transplanted limbs at postoperative day 90 was 88% in the NP and 86% in the CO + O2 3 days. The corresponding survival rate was 50% in the CO + O2 7 days at postoperative day 90 but was 0% in the SMG 3 days at postoperative day 3. Muscle mass decreased in the CO + O2 3 days and CO + O2 7 days compared with the NP, but sciatic–tibial nerve conduction velocities did not differ. These results indicate that amputated extremities preservation with hyperbaric CO and O2 could extend the time limits of preservation, maintaining their viability for replantation.
Action-effect related motor adaptation in interactions with everyday devices
Authors: Horváth János , Bíró Botond , Neszmélyi Bence 
Published: April 2018

AbstractHuman action planning relies on integrated representations of motor acts and the associated consequences, which implies that changing the set of effects associated to a motor act might directly influence action planning and control. The present study investigated the hypothesis that action-effect manipulations also affected the motor components of the actions even when only a single action option was available. Participants performed simple everyday actions (pinched a plastic sheet, pressed a button, tapped on a table) in two conditions. In the motor-auditory condition actions resulted in the presentation of a tone, whereas no tones were presented in the motor condition. The applied force was softer in the motor-auditory than in the motor condition for all three types of actions. The temporal characteristics of force application showed that action-effect related motor adaptation occurred during action planning, but possibly also during action execution. This demonstrates that even in simple, well-defined interactions with everyday devices we take all (even seemingly task-irrelevant) action-effects into account during action planning, which affects the motor component of the action. The results also imply that in experiments manipulating contingent action effects, one cannot rely on the assumption that the motor part of the action is invariant between conditions.