bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ca2+ signalling behaviours of intramuscular interstitial cells of Cajal in the murine colon
Authors: Drumm Bernard T. , Hwang Sung J. , Baker Salah A. , Ward Sean M. , Sanders Kenton M. 
Published: May 2019

Key points
Colonic intramuscular interstitial cells of Cajal (ICC‐IM) exhibit spontaneous Ca2+ transients manifesting as stochastic events from multiple firing sites with propagating Ca2+ waves occasionally observed.
Firing of Ca2+ transients in ICC‐IM is not coordinated with adjacent ICC‐IM in a field of view or even with events from other firing sites within a single cell.
Ca2+ transients, through activation of Ano1 channels and generation of inward current, cause net depolarization of colonic muscles.
Ca2+ transients in ICC‐IM rely on Ca2+ release from the endoplasmic reticulum via IP3 receptors, spatial amplification from RyRs and ongoing refilling of ER via the sarcoplasmic/endoplasmic‐reticulum‐Ca2+‐ATPase.
ICC‐IM are sustained by voltage‐independent Ca2+ influx via store‐operated Ca2+ entry.
Some of the properties of Ca2+ in ICC‐IM in the colon are similar to the behaviour of ICC located in the deep muscular plexus region of the small intestine, suggesting there are functional similarities between these classes of ICC.
AbstractA component of the SIP syncytium that regulates smooth muscle excitability in the colon is the intramuscular class of interstitial cells of Cajal (ICC‐IM). All classes of ICC (including ICC‐IM) express Ca2+‐activated Cl− channels, encoded by Ano1, and rely upon this conductance for physiological functions. Thus, Ca2+ handling in ICC is fundamental to colonic motility. We examined Ca2+ handling mechanisms in ICC‐IM of murine proximal colon expressing GCaMP6f in ICC. Several Ca2+ firing sites were detected in each cell. While individual sites displayed rhythmic Ca2+ events, the overall pattern of Ca2+ transients was stochastic. No correlation was found between discrete Ca2+ firing sites in the same cell or in adjacent cells. Ca2+ transients in some cells initiated Ca2+ waves that spread along the cell at ∼100 µm s−1. Ca2+ transients were caused by release from intracellular stores, but depended strongly on store‐operated Ca2+ entry mechanisms. ICC Ca2+ transient firing regulated the resting membrane potential of colonic tissues as a specific Ano1 antagonist hyperpolarized colonic muscles by ∼10 mV. Ca2+ transient firing was independent of membrane potential and not affected by blockade of L‐ or T‐type Ca2+ channels. Mechanisms regulating Ca2+ transients in the proximal colon displayed both similarities to and differences from the intramuscular type of ICC in the small intestine. Similarities and differences in Ca2+ release patterns might determine how ICC respond to neurotransmission in these two regions of the gastrointestinal tract.
Endovascular treatment of tandem occlusions in vertebrobasilar stroke: technical aspects and outcome compared with isolated basilar artery occlusion
Authors: Piechowiak Eike I , Kaesmacher Johannes , Zibold Felix , Dobrocky Tomas , Mosimann Pascal J , Jung Simon , Fischer Urs , Arnold Marcel , Bellwald Sebastian , Heldner Mirjam R , Gralla Jan , Mordasini Pasquale 
Published: May 2019

Background and purposeAcute basilar artery occlusion (BAO) is a devastating disease with a high rate of mortality and morbidity, and early recanalization is crucial to achieve a good prognosis. The best treatment strategy for BAO combined with a proximal stenosis (tandem occlusion) is not yet clear. Our study aimed to evaluate whether and how vertebrobasilar tandem occlusions can be treated effectively and safely compared with isolated BAO.Materials and methodsFifty-two patients with acute vertebrobasilar occlusive stroke treated with mechanical thrombectomy were identified in a prospective database from March 2010 to September 2016. We retrospectively analysed the clinical, technical, and functional outcomes of the patients.ResultsOf the 52 patients, 15 presented with a tandem occlusion, 14 with a single intracranial occlusion due to an underlying stenosis, and 23 with a single embolic BAO. Successful recanalization was achieved in all of the patients with tandem occlusions (modified Thrombolysis in Cranial Infarction 2b/3) and in 35 of 37 patients with a single BAO (with and without stenosis). A favourable outcome was achieved (modified Rankin Scale ≤2) in 8 of the 15 patients with a tandem occlusion compared with 4 of the 14 patients with a single BAO with underlying stenosis and 5 of the 23 patients with isolated embolic BAO (p=0.18).ConclusionsWe suggest that endovascular treatment of vertebrobasilar tandem occlusions is safe and feasible, with a high rate of good outcome.
Protective effects of maresin 1 against inflammation in experimentally induced acute pancreatitis and related lung injury
Authors: Munir Fahad , Jamshed Muhammad Babar , Shahid Numan , Muhammad Syed Aun , Bhandari Adheesh , Zhang QiYu 
Published: May 2019

Severe acute pancreatitis (SAP) is an inflammatory disorder that progresses with local and systemic difficulties accompanied by a relatively high mortality rate. In recent years, maresin 1 (MaR1) has been shown to be a macrophage mediator with effective proresolving and anti-inflammatory properties that prevents the occurrence of various inflammatory conditions. The purpose of this study was to investigate the role of MaR1 in SAP and related lung injury. Experimental SAP was induced in mice with a combination of cerulean and lipopolysaccharide. MaR1 was administered 30 min before the primary injection of cerulean. Biochemical markers and histological injury scores were used to evaluate the severity of acute pancreatitis. To determine the degree of inflammation, serum cytokines and myeloperoxidase activity in pancreas and lung tissues were measured. Western blot analysis detected the activation of NF-κB. After MaR1 pretreatment, the activities of amylase, lipase, TNF-α, IL-1β, and IL-6 were decreased in serum, and the myeloperoxidase activity both in pancreas and in lung tissues significantly decreased, whereas the activity of anti-inflammatory cytokine IL-10 in serum was increased. MaR1-pretreated mice reduced the activation of pancreatic NF-κB and decreased the severity of pancreatic and lung-related injuries. These results confirm that MaR1 alleviated inflammation of the pancreas and lung by inhibiting the activity of NF-κB in experimentally induced acute pancreatitis and exerted anti-inflammatory effects. These findings suggest that MaR1 could be a new and useful drug in the treatment of SAP. NEW & NOTEWORTHY These results provided us evidence to confirm that maresin 1 (MaR1) can alleviate inflammation of the pancreas and lung by inhibiting the activity of NF-κB in experimental induced acute pancreatitis and exerts certain anti-inflammatory effects. These findings suggest that MaR1 could be a new and useful drug in the treatment of severe acute pancreatitis.
Human recombinant relaxin-2 does not attenuate hypertension or renal injury but exacerbates vascular dysfunction in a female mouse model of SLE
Authors: Wolf Victoria L. , Phillips Taylor L. , Taylor Erin B. , Sasser Jennifer M. , Ryan Michael J. 
Published: May 2019

Systemic lupus erythematosus (SLE) is an autoimmune disease that disproportionately affects women of reproductive age and increases their risk for developing hypertension, vascular, and renal disease. Relaxin has potential beneficial therapeutic effects in cardiovascular disease through direct actions on the vasculature. The potential therapeutic benefit of relaxin on SLE-associated cardiovascular and renal risk factors like hypertension has not previously been tested. We hypothesized that relaxin would attenuate hypertension, renal injury, and vascular dysfunction in an established female mouse model of SLE (NZBWF1 mice). Serelaxin (human recombinant relaxin-2, 0.5 mg·kg−1·day−1) or vehicle was administered via osmotic mini-pump for 4 wk in female control (NZW) or SLE mice between 28 and 31 wk of age. Serelaxin treatment increased uterine weights in both groups, suggesting that the Serelaxin was bioactive. Mean arterial pressure, measured by carotid artery catheter, was significantly increased in vehicle-treated SLE mice compared with vehicle-treated controls, but was not changed by Serelaxin treatment. Albumin excretion rate, measured by ELISA, was similar between vehicle- and Serelaxin-treated SLE mice and between vehicle- and Serelaxin-treated control mice. Wire myography was performed using isolated carotid arteries to assess endothelial-independent and -dependent vasodilation, and data confirm that SLE mice have impaired endothelium-independent and -dependent relaxation compared with control mice. Serelaxin treatment did not affect endothelium-independent vasodilation, but exacerbated the endothelium-dependent dysfunction. These data suggest that, contrary to our hypothesis, Serelaxin infusion does not attenuate hypertension, renal injury, or vascular dysfunction in SLE, but worsens underlying vascular endothelial dysfunction in this experimental model of SLE. These data do not support the use of human recombinant relaxin-2 as an antihypertensive in the SLE patient population. NEW & NOTEWORTHY Relaxin is a peptide hormone commonly known for its role in pregnancy and for its use in recent clinical trials for the treatment of heart failure. Evidence suggests that relaxin has immunomodulatory effects; however, the potential therapeutic impact of relaxin in chronic immune mediated disease is unclear. This study tests whether recombinant human relaxin (Serelaxin) attenuates the progression of autoimmunity, and the associated cardiovascular consequences, in an experimental model of systemic lupus erythematosus.
Dietary Calanus oil recovers metabolic flexibility and rescues postischemic cardiac function in obese female mice
Authors: Jansen Kirsten M. , Moreno Sonia , Garcia-Roves Pablo M. , Larsen Terje S. 
Published: May 2019

The aim of this study was to find out whether dietary supplementation with Calanus oil (a novel marine oil) or infusion of exenatide (an incretin mimetic) could counteract obesity-induced alterations in myocardial metabolism and improve postischemic recovery of left ventricular (LV) function. Female C57bl/6J mice received high-fat diet (HFD, 45% energy from fat) for 12 wk followed by 8-wk feeding with nonsupplemented HFD, HFD supplemented with 2% Calanus oil, or HFD plus exenatide infusion (10 µg·kg−1·day−1). A lean control group was included, receiving normal chow throughout the whole period. Fatty acid and glucose oxidation was measured in ex vivo perfused hearts during baseline conditions, while LV function was assessed with an intraventricular fluid-filled balloon before and after 20 min of global ischemia. HFD-fed mice receiving Calanus oil or exenatide showed less intra-abdominal fat deposition than mice receiving nonsupplemented HFD. Both treatments prevented the HFD-induced decline in myocardial glucose oxidation. Somewhat surprising, recovery of LV function was apparently better in hearts from mice fed nonsupplemented HFD relative to hearts from mice fed normal chow. More importantly however, postischemic recovery of hearts from mice receiving HFD with Calanus oil was superior to that of mice receiving nonsupplemented HFD and mice receiving HFD with exenatide, as expressed by better pressure development, contractility, and relaxation properties. In summary, dietary Calanus oil and administration of exenatide counteracted obesity-induced derangements of myocardial metabolism. Calanus oil also protected the heart from ischemia, which could have implications for the prevention of obesity-related cardiac disease. NEW & NOTEWORTHY This article describes for the first time that dietary supplementation with a low amount (2%) of a novel marine oil (Calanus oil) in mice is able to prevent the overreliance of fatty acid oxidation for energy production during obesity. The same effect was observed with infusion of the incretin mimetic, exanatide. The improvement in myocardial metabolism in Calanus oil-treated mice was accompanied by a significantly better recovery of cardiac performance following ischemia-reperfusion. Listen to this article’s corresponding podcast at https://ajpheart.podbean.com/e/dietary-calanus-oil-energy-metabolism-and-cardiac-function/ .
BCMA-Targeted CAR T-cell Therapy plus Radiotherapy for the Treatment of Refractory Myeloma Reveals Potential Synergy
Authors: Smith Eric L. , Mailankody Sham , Staehr Mette , Wang Xiuyan , Senechal Brigitte , Purdon Terence J. , Daniyan Anthony F. , Geyer Mark B. , Goldberg Aaron D. , Mead Elena , Santomasso Bianca D. , Landa Jonathan , Rimner Andreas , Riviere Isabelle , Landgren Ola , Brentjens Renier J. 
Published: May 2019

Abstract
We present a case of a patient with multiply relapsed, refractory myeloma whose clinical course showed evidence of a synergistic abscopal-like response to chimeric antigen receptor (CAR) T-cell therapy and localized radiotherapy (XRT). Shortly after receiving B-cell maturation antigen (BCMA)–targeted CAR T-cell therapy, the patient required urgent high-dose steroids and XRT for spinal cord compression. Despite the steroids, the patient had a durable systemic response that could not be attributed to XRT alone. Post-XRT findings included a second wave of fever and increased CRP and IL6, beginning 21 days after CAR T cells, which is late for cytokine-release syndrome from CAR T-cell therapy alone on this trial. Given this response, which resembled cytokine-release syndrome, immediately following XRT, we investigated changes in the patient's T-cell receptor (TCR) repertoire over 10 serial time points. Comparing T-cell diversity via Morisita's overlap indices (CD), we discovered that, although the diversity was initially stable after CAR T-cell therapy compared with baseline (CD = 0.89–0.97, baseline vs. 4 time points after CAR T cells), T-cell diversity changed after the conclusion of XRT, with &gt;30% newly expanded TCRs (CD = 0.56–0.69, baseline vs. 4 time points after XRT). These findings suggest potential synergy between radiation and CAR T-cell therapies resulting in an abscopal-like response.
Two new species of Pseudapis Kirby, 1900 (Hymenoptera: Halictidae: Nomiinae) from Africa
Authors: BOSSERT SILAS , PAULY ALAIN 
Published: May 2019

Two previously unknown species of the genus Pseudapis Kirby, 1900 are described and illustrated: Pseudapis neumayeri Bossert & Pauly, sp. nov. (♂, ♀, Kenya, Tanzania), and P. crassivertex Bossert & Pauly, sp. nov. (♂, ♀, Mali, Mauritania, Niger, Senegal). We provide diagnoses for the new species and report all currently available records. Lastly, we present a revised identification key for the African species of the genus.
Communicating Hurricane Risk with Virtual Reality: A Pilot Project
Authors: Bernhardt Jase , Snellings Jackson , Smiros Alexander , Bermejo Ivan , Rienzo Angela , Swan Carys 
Published: May 2019

AbstractLandfalling hurricanes in the United States can inflict extreme damage and loss of life. The latter, particularly, can be caused by a host of socioeconomic factors, including insufficient understanding of risk by individuals expected to be impacted by the storm. Thus, we test the use of an emerging technology, virtual reality (VR), to enhance the communication of real-time risk from a hurricane forecast to make landfall. In this pilot study, individuals are presented with a hypothetical scenario where a major hurricane is forecast to impact their community within 48–72 h. The survey includes two different types of warning products related to the hypothetical hurricane: static text and maps emulating those traditionally used by media outlets and local officials to communicate risk, and a VR video simulating a hurricane landfall in a residential neighborhood. We survey two groups of equal size (each n = 62), one viewing both the VR simulation and traditional products, and the other only the latter. Each group was then asked a series of Likert-scale and open-ended questions to assess the effectiveness of both products. We determine that participants viewing both the VR and traditional products are significantly more likely to take action in preparation for the hypothetical landfall than those being exposed to just the traditional products. These results demonstrate that VR can be a useful component of hurricane warning products, and further work can be done to improve the effectiveness of such products and assess how broader segments of the population can access this information.
Research on urban anti-terrorism intelligence perception system from the perspective of Internet of things application
Authors: Xi Meng , Lingyu Nie , Jiapeng Song 
Published: May 2019

Objective/significance Based on the urgent needs of the status quo of urban anti-terrorism, combining the present situation of intelligent city construction, we carry out the research on the key problems such as the collection and analysis of counter-terrorism information for urban environment. This paper puts forward a kind of city anti-terrorism information perception system based on the Internet of things, and realizes to improve the level of surveillance and early warning of terrorist activities facing the urban environment. Method/Process The system collects and merges the heterogeneous information of terrorism, which is based on the information perception layer, the intelligence identification layer and the intelligence inference layer, which is based on the city's focal area. To filter suspicious information by constructing a semantic database of terrorist action signals Combined with complex event processing and machine learning technology, real-time monitoring and mining prediction of mass event flow, using the comparison between historical data of terrorist attacks and real-time data, increased the area of counter-terrorism intelligence. Results/Conclusions We hope to provide a better model and an exploratory study on the construction of the counter-terrorism early warning for the urban environment.
Assessing professional competence: a critical review of the Annual Review of Competence Progression
Authors: Woolf Katherine , Page Michael , Viney Rowena 
Published: May 2019

The Annual Review of Competence Progression is used to determine whether trainee doctors in the United Kingdom are safe and competent to progress to the next training stage. In this article we provide evidence to inform recommendations to enhance the validity of the summative and formative elements of the Annual Review of Competency Progression. The work was commissioned as part of a Health Education England review. We systematic searched the peer reviewed and grey literature, synthesising findings with information from national, local and specialty-specific Annual Review of Competence Progression guidance, critically evaluating the findings in the context of literature on assessing competence in medical education. National guidance lacked detail resulting in variability across locations and specialties, threatening validity and reliability. Trainees and trainers were concerned that the Annual Review of Competence Progression only reliably identifies the most poorly performing trainees. Feedback is not routinely provided, which can leave those with performance difficulties unsupported and high performers demotivated. Variability in the provision and quality of feedback can negatively affect learning. The Annual Review of Competence Progression functions as a high-stakes assessment, likely to have a significant impact on patient care. It should be subject to the same rigorous evaluation as other high-stakes assessments; there should be consistency in procedures across locations, specialties and grades; and all trainees should receive high-quality feedback.
Identification of potential long noncoding RNA associated with nasopharyngeal carcinoma using deep sequencing
Authors: Xu Yu-Zhong , Chen Fang-Fang , Zhang Yu , Liang Hui , Li Xiao-Jun , He Chen 
Published: May 2019

Objective To identify differentially expressed long noncoding RNAs (lncRNAs) in nasopharyngeal carcinoma (NPC) compared with chronic nasopharyngitis (CNP) tissues. Methods This prospective cohort study enrolled patients with NPC and CNP. The levels of lncRNAs in NPC and CNP tissues was detected by deep sequencing and quantitative polymerase chain reaction. Kyoto Encyclopedia of Genes and Genomes pathway analysis and antisense prediction were performed to reveal the function of differentially expressed lncRNAs (DELs). Receiver operating characteristic (ROC) curve and correlation analyses were used to evaluate the clinical and prognostic value of lncRNAs. Results A total of 30 NPC and 27 CNP tissues were analysed. A total of 296 DELs were identified. LncRNAs ENSG00000227084 and ENSG00000230489 might play important roles in the development of NPC by interfering with the Rap1 signalling pathway and natural killer cell-mediated cytotoxicity, respectively. Antisense prediction showed that lncRNA ENSG00000230489 was paired with VAV3 mRNA. LncRNAs ENSG00000230489 (area under the ROC curve = 0.9138) and ENSG00000227084 (area under the ROC curve = 0.8037) may be diagnostic markers for NPC. Furthermore, low levels of ENSG00000230489 was positively associated with distant metastasis. Conclusion LncRNAs ENSG00000230489 and ENSG00000227084 may be potential diagnostic markers and therapeutic targets for NPC.