bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extending the Indication for Laparoscopic Surgery in Patients With Pan-Peritonitis
Authors: Yeom Jong Hoon , Lee Jun Ho , Song Joon Sung , Lee Min Hong , Kim Min Gyu 
Published: December 2018

Background:
There have been reports of favorable results in patients with peritonitis who underwent laparoscopic surgery. The present study evaluated the outcomes of laparoscopic surgery in patients with pan-peritonitis.

Materials and Methods:
We enrolled 148 patients who underwent open and laparoscopic surgery for pan-peritonitis between January 2015 and December 2017 at Hanyang University Guri Hospital. Patients with appendicitis or cholecystitis were excluded from this study because of the simplicity of the technique and the favorable postoperative prognosis. Surgical outcomes including open conversion, morbidity, mortality, risk, and prognostic factors were evaluated.

Results:
During the study period, 125 patients (84.5%) underwent laparoscopic surgery. The conversion to open surgery occurred in 7 (5.3%) patients. In the laparoscopic surgery group, 27 patients (21.6%) experienced postoperative complications, and 6 died (4.8%). Multivariate analysis showed that age of older than 75 years was a risk factor for postoperative complications. Especially, preoperative shock (<90 mm Hg) and a longer time from symptom onset (over 2 d) to surgery were prognostic factors for postoperative mortality.

Conclusions:
On the basis of our results and experiences, we believe that laparoscopic surgery is applicable to surgery of pan-peritonitis. And, preoperative shock and longer time from onset are prognostic factors of mortality in laparoscopic group. Therefore, we are confident that careful patient selection and specialized laparoscopic team should be considered in order to have best results.
Dynamics of microbial contaminants is driven by selection during ethanol production
Authors: Queiroz Luciano Lopes , Costa Maria Silveira , de Abreu Pereira Alcilene , de Paula Avila Marcelo , Costa Patrícia Silva , Nascimento Andréa Maria Amaral , Lacorte Gustavo Augusto 
Published: December 2018

AbstractBrazil is the second largest ethanol producer in the World and largest using sugarcane feedstock. Bacteria contamination is one the most important issues faced by ethanol producers that seek to increase production efficiency. Each step of production is a selection event due to the environmental and biological changes that occur. Therefore, we evaluated the influence of the selection arising from the ethanol production process on diversity and composition of Bacteria. Our objectives were to test two hypothesis, (1) that species richness will decrease during the production process and (2) that Lactic Acid Bacteria will become dominant with the advance of ethanol production due to selection. Bacterial community assemblage was accessed using 16S rRNA gene sequencing from 19 sequential samples. Temperature is of great importance in shaping microbial communities. Species richness increased between the Decanter and Must steps of the process. Low Simpson index values were recorded at the fermentation step, indicating a high dominance ofLactobacillus. Interactions betweenLactobacillusand yeast may be impairing the efficiency of industrial ethanol production.
Novel Alzheimer risk genes determine the microglia response to amyloid-β but not to TAU pathology
Authors: Sierksma Annerieke , Lu Ashley , Salta Evgenia , Mancuso Renzo , Zoco Jesus , Blum David , Buée Prof Luc , Strooper Prof Bart De , Fiers Mark 
Published: December 2018

AbstractBackgroundThousands of SNPs associated with risk of Alzheimer’s disease (AD) in genome-wide association studies (GWAS) do not reach genome-wide significance. When combined, they contribute however to a highly predictive polygenic risk score. The relevance of these subthreshold risk genes to disease, and how their combined predictive power translates into functionally relevant disease pathways, is unknown. We investigate here at the genome-wide level and in an unbiased way to what extent AD risk genes show altered gene expression in the context of increasing Aβ or Tau pathology in mouse models of AD.MethodsWe used an existing GWAS data set to generate lists of candidate AD genes at different levels of significance. We performed transcriptomic analysis on wild-type and transgenic APP/PS1 (APPtg) and Thy-TAU22 (TAUtg) mouse models at early and late stage of disease. We used unbiased weighted gene co-expression network analysis (WGCNA) to identify clusters of co-regulated genes responsive to Aβ or TAU pathology. Gene set enrichment was used to identify clusters that were enriched for AD risk genes.FindingsConsistent and significant enrichment of AD risk genes was found in only one out of 63 coexpression modules. This module is highly responsive to Aβ but not to TAU pathology. We identify in this module 18 AD risk genes (p-value=6·5e-11) including 11 new ones, GPC2, TREML2, SYK, GRN, SLC2A5, SAMSN1, PYDC1, HEXB, RRBP1, LYN and BLNK. All are expressed in microglia, have a binding site for the transcription factor SPI1 (PU.1), and become significantly upregulated when exposed to Aβ. A subset regulates FC-gamma receptor mediated phagocytosis.InterpretationGenetic risk of AD is functionally translated into a microglia pathway responsive to Aβ pathology. This insight integrates aspects of the amyloid hypothesis with genetic risk associated to sporadic AD.
Analyzing the neuroglial brainstem circuits for respiratory chemosensitivity in freely moving mice
Authors: Bhandare Amol , van de Wiel Joseph , Roberts Reno , Braren Ingke , Huckstepp Robert , Dale Nicholas 
Published: December 2018

AbstractThe regulated excretion of CO2 during breathing is a key life-preserving homeostatic mechanism. In the rostral medulla oblongata, neurons in two nuclei -the retrotrapezoid nucleus (RTN) and the rostral medullary Raphe -have been proposed as central CO2 chemosensors that mediate adaptive changes in breathing. One such adaptive change is active expiration, thought to be controlled by the lateral parafacial region (pFL). Here we use promoter-driven expression of GCaMP6 and head-mounted mini-microscopes to image the Ca2+ activity in neurons and glia of RTN, Raphe and pFL in awake adult mice during inspiration of elevated CO2 to discriminate their roles in the adaptive response to hypercapnia. While there were multiple types of neuronal response to hypercapnia in both the RTN and the Raphe, a substantial proportion of neurons in the Raphe encoded the inspired level of CO2. By contrast, such responses were very rare in the RTN and the responses of RTN neurons suggest that this nucleus plays an integrative rather than a direct sensing role in the chemosensory reflex. pFL neurons were reliably activated by hypercapnia and their activity preceded active expiration suggesting a key causal link to this aspect of breathing. Our analysis considerably revises understanding of chemosensory control in the adult awake mouse and paves the way to understanding how breathing is coordinated with complex non-ventilatory behaviours such as swallowing and vocalisation.
Arabidopsis Myosins XI Are Involved in Exocytosis of Cellulose Synthase Complexes
Authors: Zhang Weiwei , Cai Chao , Staiger Christopher J. 
Published: December 2018

ABSTRACTIn plants, cellulose is synthesized at the cell surface by plasma membrane (PM)-localized cellulose synthase (CESA) complexes (CSCs). The molecular and cellular mechanisms that underpin delivery of CSCs to the PM, however, are poorly understood. Cortical microtubules have been shown to interact with CESA-containing compartments and mark the site for CSC delivery, but are not required for the delivery itself. Here, we demonstrate that myosin XI and the actin cytoskeleton mediate CSC delivery to the PM by coordinating the exocytosis of CESA-containing compartments. Measurement of cellulose content indicated that cellulose biosynthesis was significantly reduced in a myosin xik xi1 xi2 triple knockout (xi3KO) mutant. By combining genetic and pharmacological disruption of myosin activity with quantitative live-cell imaging, we observed decreased abundance of PM-localized CSCs and reduced delivery rate of CSCs in myosin-deficient cells. These phenotypes correlated with a significant increase in failed vesicle secretion events at the PM as well as an abnormal accumulation of CESA-containing compartments at the cell cortex. Through high spatiotemporal assays of cortical vesicle behavior, we identified defects in CSC vesicle tethering and fusion at the PM. Furthermore, disruption of myosin activity reduced the delivery of several other secretory markers to the PM and reduced constitutive and receptor-mediated endocytosis. These findings reveal a previously undescribed role for myosin in vesicle secretion and cellulose production at the cytoskeleton–PM–cell wall nexus.
A novel characteristic variational multiscale FEM for incompressible natural convection problem with variable density
Authors: Wang Weilong , Wu Jilian , Feng Xinlong 
Published: December 2018

PurposeThe purpose of this paper is to propose a new method to solve the incompressible natural convection problem with variable density. The main novel ideas of this work are to overcome the stability issue due to the nonlinear inertial term and the hyperbolic term for conventional finite element methods and to deal with high Rayleigh number for the natural convection problem.Design/methodology/approachThe paper introduces a novel characteristic variational multiscale (C-VMS) finite element method which combines advantages of both the characteristic and variational multiscale methods within a variational framework for solving the incompressible natural convection problem with variable density. The authors chose the conforming finite element pair (P2,P2,P1,P2) to approximate the density, velocity, pressure and temperature field.FindingsThe paper gives the stability analysis of the C-VMS method. Extensive two-dimensional/three-dimensional numerical tests demonstrated that the C-VMS method not only can deal with the incompressible natural convection problem with variable density but also with high Rayleigh number very well.Originality/valueExtensive 2D/3D numerical tests demonstrated that the C-VMS method not only can deal with the incompressible natural convection problem with variable density but also with high Rayleigh number very well.
Connection or competence
Authors: Moreo Andrew , Woods Robert , Sammons Gail , Bergman Christine 
Published: December 2018

Purpose
As a service segment, the food and beverage industry has great potential for intensive interface between the consumer and the service provider and between the service provider and the back of the house staff. Given the significance of the perception of the provision of service to the consumer, it is important to study every aspect of the interaction. With this in mind, the purpose of this study is to examine the relationships between emotional labor, service quality, purpose of consumption, satisfaction and customer loyalty as seen through the perceptions of the consumer.

Design/methodology/approach
Survey data were collected from 400 respondents using an online market research firm in the USA. This research used three independent variables (emotional labor, service quality and purpose of consumption), each with two levels in a between-subjects 2 × 2 × 2 factorial experimental design. Eight scenarios were used to represent the eight different combinations of variables to test their effects.

Findings
Two very interesting findings emerged from this study. The first was that consumers’ purpose of consumption, whether dining for leisure or business, had no significant impact on their satisfaction or loyalty, either independently or in conjunction with emotional labor or service quality. The second most interesting finding was that the interaction of service quality and emotional labor did have a significant impact on satisfaction but not loyalty.

Practical implications
A restaurants’ standards of service should be the same, regardless of the business or leisure demographic being served. Authenticity matters, and therefore, employers should incorporate personality evaluation into the hiring process. Perhaps, focusing more on attitude and less on skill would lead to great customer satisfaction and loyalty.

Originality/value
This article furthers the relatively young research stream examining the impacts of emotional labor on the consumer.
A scale for restaurant customers’ healthy menu choices: individual and environmental factors
Authors: Jeong EunHa , Jang SooCheong (Shawn) , Behnke Carl , Anderson James , Day Jonathon 
Published: December 2018

Purpose
The purpose of this study is to explore the dimensions of restaurant customers’ engagement or disengagement with healthy eating in terms of individual and environmental factors to develop a scale. The results identified the underlying constructs of customers’ individual motives for and perceived barriers to healthy eating, as well as environmental elements of restaurants that encourage or discourage healthy eating.

Design/methodology/approach
To develop an appropriate set of measures to assess factors influencing customers’ healthy eating behaviors at restaurants, the current study undertook the five steps of scale development suggested by Churchill (1979): specifying the domain of constructs, generating a pool of initial measurement items, assessing content adequacy, administering questionnaires (an online survey method) and purifying and finalizing the measurement (via exploratory factor analysis (EFA) using 410 samples and confirmatory factor analysis (CFA) using 423 samples).

Findings
The results revealed ten individual factors (health, body image, weight control, feeling better, unappealing food, cost perception, lack of knowledge, state of mind (stress), lack of self-control and negative influences) and five environmental factors (healthy indications, social impact, availability of healthy menu, price policy and unhealthy indications) influencing customers’ healthy eating behaviors at restaurants.

Originality/value
This study developed an appropriate set of measures to assess individual and environmental factors influencing restaurant customers’ healthy eating behaviors, along with identifying underlying sub-constructs. The reliability and validity of the scale and the factor structure are presented and potential applications and theoretical contributions of the scale are provided as well.
Ontology-based approach to enhance medical web information extraction
Authors: Otmani Nassim Abdeldjallal , Si-Mohammed Malik , Comparot Catherine , Charrel Pierre-Jean 
Published: December 2018

Purpose
The purpose of this study is to propose a framework for extracting medical information from the Web using domain ontologies. Patient–Doctor conversations have become prevalent on the Web. For instance, solutions like HealthTap or AskTheDoctors allow patients to ask doctors health-related questions. However, most online health-care consumers still struggle to express their questions efficiently due mainly to the expert/layman language and knowledge discrepancy. Extracting information from these layman descriptions, which typically lack expert terminology, is challenging. This hinders the efficiency of the underlying applications such as information retrieval. Herein, an ontology-driven approach is proposed, which aims at extracting information from such sparse descriptions using a meta-model.

Design/methodology/approach
A meta-model is designed to bridge the gap between the vocabulary of the medical experts and the consumers of the health services. The meta-model is mapped with SNOMED-CT to access the comprehensive medical vocabulary, as well as with WordNet to improve the coverage of layman terms during information extraction. To assess the potential of the approach, an information extraction prototype based on syntactical patterns is implemented.

Findings
The evaluation of the approach on the gold standard corpus defined in Task1 of ShARe CLEF 2013 showed promising results, an F-score of 0.79 for recognizing medical concepts in real-life medical documents.

Originality/value
The originality of the proposed approach lies in the way information is extracted. The context defined through a meta-model proved to be efficient for the task of information extraction, especially from layman descriptions.
Nurse AMHPs: an exploratory study of their experiences
Authors: Stone Kevin 
Published: December 2018

Purpose
Mental health and learning disability nurses have been eligible to become approved mental health professionals (AMHPs) since 2008, when the Mental Health Act 2007 was implemented. Despite this, there have been proportionally low numbers of these nurses pursuing the AMHP role. The purpose of this paper is to explore the experiences of these nurse AMHPs of training and practice.

Design/methodology/approach
Ten practicing nurse AMHPs were recruited from across four local authority sites. Using semi-structured interviews, participants were asked to discuss their experiences of being an AMHP.

Findings
The participants highlighted the need to navigate personal, cultural and structural factors relating to accessing and applying for the training, difficulties with agreeing contracts terms, gaining comparative pay and undertaking the role.

Research limitations/implications
The limitations of this study are the small number of participants and therefore the generalisability of the findings. Also, respondents were practising AMHPs rather than nurses who considered the role but then rejected it as a career option.

Practical implications
This study has led to gain a greater understanding of the experiences of nurse AMHPs.

Social implications
The results from this study will assist employing local authorities, and NHS consider the barriers to mental health and learning disability nurses becoming AMHPs.

Originality/value
The value of this study is in the insight that provides the experiences of nurse AMHP from applying to training through to being a practising AMHP.
The Effects of High Dynamic Range on Fingerprint Images Processed by Photoshop
Authors: Chuk Hungngai 
Published: December 2018

AbstractHigh dynamic range (HDR) imaging is a function that combines five images with different exposures into a single image. This technique may provide fine ridge details of fingerprint images for forensic latent fingerprint examination. Therefore, viewing fingerprints under optimal conditions is of paramount importance. This paper analyzes HDR and non‐HDR photos by using the Michelson contrast formula. The Michelson formula will provide a measurement to determine whether better contrast between the background and print can be achieved using the HDR function and if the background color affects the quality of the images. Two hypothesis were tested: (i) the HDR image provides more details of fingerprints with a better tone, greater clarity, and contrast than a normally exposed image regardless of the background color; (ii) the background color does not affect the quality of HDR fingerprint images overall, but the multi‐color background may increase the contrast of HDR fingerprint images in some cases.
Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs
Authors: Dorta Sílvia de Oliveira , Balbinotte Juliana , Monnerat Rose , Lopes João Roberto Spotti , da Cunha Tatiane , Zanardi Odimar Zanuzo , de Miranda Marcelo Pedreira , Machado Marcos Antonio , de Freitas‐Astúa Juliana 
Published: December 2018

AbstractBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap‐sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of “Candidatus Liberibacter spp.,” associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil‐drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild‐type Bt strains against D. citri third‐instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%–77% and 66%–90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil‐drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus.
Deciphering global gene expression and regulation strategy in Escherichia coli during carbon limitation
Authors: Li Zongjin , Pan Qing , Xiao Yunzhu , Fang Xingxing , Shi Ruoping , Fu Chunxiang , Danchin Antoine , You Conghui 
Published: December 2018

SummaryDespite decades of studies meant to analyse the bacterial response to carbon limitation, we still miss a high‐resolution overview of the situation. All gene expression changes observed in such conditions cannot solely be accounted for by the global regulator Crp either free or bound to its effector, cyclic AMP. Here, for the first time, we evaluated the response of both CDS (protein‐coding sequence) and ncRNA (non‐coding RNA) genes to carbon limitation, revealed cellular functions of differentially expressed genes systematically, quantified the contribution of Crp‐cAMP and other factors to regulation and deciphered regulation strategies at a genomewide scale. Approximately one‐third of the differentially expressed genes we identified responded to Crp‐cAMP via its direct or indirect control, while the remaining genes were subject to growth rate‐dependent control or were controlled by other regulators, especially RpoS. Importantly, gene regulation mechanisms can be established by expression pattern studies. Here, we propose a comprehensive picture of how cells respond to carbon scarcity. The global regulation strategies thus exposed illustrate that the response of cell to carbon scarcity is not limited to maintaining sufficient carbon metabolism via cAMP signalling while the main response is to adjust metabolism to cope with a slow growth rate.