bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome Analysis of Litsea cubeba Floral Buds Reveals the Role of Hormones and Transcription Factors in the Differentiation Process
Authors: He Wenguang , Chen Yicun , Gao Ming , Zhao Yunxiao , Xu Zilong , Cao Pei , Zhang Qiyan , Jiao Yulian , Li Hongsheng , Wu Liwen , Wang Yangdong 
Published: February 2018

Abstract

Background
Litsea cubeba (Lour.) Pers. is an important economic plant that is rich in valuable essential oil. The essential oil is often used as a raw material for perfumes, food additives, insecticides and bacteriostats. Most of the essential oil is contained in the fruit, and the quantity and quality of fruit are dependent on the flowers. To explore the molecular mechanism of floral bud differentiation, high-throughput RNA sequencing was used to detect differences in the gene expression of L. cubeba female and male floral buds at three differentiation stages.

Results
This study obtained 160.88 Gbp of clean data that were assembled into 100,072 unigenes, and a total of 38,658 unigenes were annotated. A total of 27,521 simple sequence repeats (SSRs) were identified after scanning the assembled transcriptome, and the mono-nucleotide repeats were predominant, followed by di-nucleotide and tri-nucleotide repeats. A total of 12,559 differentially expressed genes (DEGs) were detected from the female (F) and male (M) floral bud comparisons. The gene ontology (GO) databases revealed that these DEGs were primarily contained in “metabolic processes”, “cellular processes”, and “single-organism processes”. The Kyoto Encyclopedia of Genes and Genomes (KEGG) databases suggested that the DEGs belonged to “plant hormone signal transduction” and accounted for a relatively large portion in all of these comparisons. We analyzed the expression level of plant hormone-related genes and detected the contents of several relevant plant hormones in different stages. The results revealed that the dynamic changes in each hormone content were almost consistent with the expression levels of relevant genes. The transcription factors selected from the DEGs were analyzed. Most DEGs of MADS-box were upregulated and most DEGs of bZIP were downregulated. The expression trends of the DEGs were nearly identical in female and male floral buds, and qRT-PCR analysis revealed consistency with the transcriptome data.

Conclusions
We sequenced and assembled a high-quality L. cubeba floral bud transcriptome, and the data appeared to be well replicated (n = 3) over three developmental time points during flower development. Our study explored the changes in the contents of several plant hormones during floral bud differentiation using biochemical and molecular biology techniques, and the changes in expression levels of several flower development related transcription factors. These results revealed the role of these factors (i.e., hormones and transcription factors) and may advance our understanding of their functions in flower development in L. cubeba.
Genetic Interactions BetweenBOB1and Multiple 26S Proteasome Subunits Suggest a Role for Proteostasis in RegulatingArabidopsisDevelopment
Authors: Silverblatt-Buser Elan W , Frick Melissa A , Rabeler Christina , Kaplinsky Nicholas J 
Published: February 2018

AbstractProtein folding and degradation are both required for protein quality control, an essential cellular activity that underlies normal growth and development. We investigated how BOB1, an Arabidopsis thaliana small heat shock protein, maintains normal plant development. bob1 mutants exhibit organ polarity defects and have expanded domains of KNOX gene expression. Some of these phenotypes are ecotype specific suggesting that other genes function to modify them. Using a genetic approach we identified an interaction between BOB1 and FIL, a gene required for abaxial organ identity. We also performed an EMS enhancer screen using the bob1-3 allele to identify pathways that are sensitized by a loss of BOB1 function. This screen identified genetic, but not physical, interactions between BOB1 and the proteasome subunit RPT2a. Two other proteasome subunits, RPN1a and RPN8a, also interact genetically with BOB1. Both BOB1 and the BOB1-interacting proteasome subunits had previously been shown to interact genetically with the transcriptional enhancers AS1 and AS2, genes known to regulate both organ polarity and KNOX gene expression. Our results suggest a model in which BOB1 mediated protein folding and proteasome mediated protein degradation form a functional proteostasis module required for ensuring normal plant development.
Accounting for Errors in Low Coverage High-Throughput Sequencing Data When Constructing Genetic Maps Using Biparental Outcrossed Populations
Authors: Bilton Timothy P , Schofield Matthew R , Black Michael A , Chagné David , Wilcox Phillip L , Dodds Ken G 
Published: February 2018

AbstractNext generation sequencing-based genotyping platforms allow for the construction of high density genetic linkage maps. However, data generated using these platforms often contain errors resulting from miscalled bases and missing parental alleles that are due...Next-generation sequencing is an efficient method that allows for substantially more markers than previous technologies, providing opportunities for building high-density genetic linkage maps, which facilitate the development of nonmodel species’ genomic assemblies and the investigation of their genes. However, constructing genetic maps using data generated via high-throughput sequencing technology (e.g., genotyping-by-sequencing) is complicated by the presence of sequencing errors and genotyping errors resulting from missing parental alleles due to low sequencing depth. If unaccounted for, these errors lead to inflated genetic maps. In addition, map construction in many species is performed using full-sibling family populations derived from the outcrossing of two individuals, where unknown parental phase and varying segregation types further complicate construction. We present a new methodology for modeling low coverage sequencing data in the construction of genetic linkage maps using full-sibling populations of diploid species, implemented in a package called GUSMap. Our model is based on the Lander–Green hidden Markov model but extended to account for errors present in sequencing data. We were able to obtain accurate estimates of the recombination fractions and overall map distance using GUSMap, while most existing mapping packages produced inflated genetic maps in the presence of errors. Our results demonstrate the feasibility of using low coverage sequencing data to produce genetic maps without requiring extensive filtering of potentially erroneous genotypes, provided that the associated errors are correctly accounted for in the model.
Age-related physical and psychological vulnerability as pathways to problem gambling in older adults
Authors: Parke Adrian , Griffiths Mark , Pattinson Julie , Keatley David 
Published: February 2018

Background
To inform clinical treatment and preventative efforts, there is an important need to understand the pathways to late-life gambling disorder.

Aims
This study assesses the association between age-related physical health, social networks, and problem gambling in adults aged over 65 years and assesses the mediating role of affective disorders in this association.

Methods
The sample comprised 595 older adults (mean age: 74.4 years, range: 65–94 years; 77.1% female) who were interviewed using a structured questionnaire to assess physical frailty, geriatric pain, loneliness, geriatric depression, geriatric anxiety, and problem gambling.

Results
Pathway analysis demonstrated associations between these variables and gambling problems, providing a good fit for the data, but that critically these relationships were mediated by both anxiety and depression symptoms.

Conclusions
This study indicates that late-life problem gambling may develop as vulnerable individuals gamble to escape anxiety and depression consequent to deteriorating physical well-being and social support. When individuals develop late-life problem gambling, it is recommended that the treatment primarily focuses upon targeting and replacing avoidant coping approaches.
Ki67 Changes Identify Worse Outcomes in Residual Breast Cancer Tumors After Neoadjuvant Chemotherapy
Authors: Cabrera-Galeana Paula , Muñoz-Montaño Wendy , Lara-Medina Fernando , Alvarado-Miranda Alberto , Pérez-Sánchez Victor , Villarreal-Garza Cynthia , Quintero R. Marisol , Porras-Reyes Fany , Bargallo-Rocha Enrique , Del Carmen Ignacio , Mohar Alejandro , Arrieta Oscar 
Published: February 2018

Abstract

Background
Several breast cancer (BC) trials have adopted pathological complete response (pCR) as a surrogate marker of long-term treatment efficacy. In patients with luminal subtype, pCR seems less important for outcome prediction. BC is a heterogeneous disease, which is evident in residual tumors after neoadjuvant-chemotherapy (NAC). This study evaluates changes in Ki67 in relation to disease-free survival (DFS) and overall survival (OS) in patients without pCR.

Subjects, Materials, and Methods
Four hundred thirty-five patients with stage IIA–IIIC BC without pCR after standard NAC with anthracycline and paclitaxel were analyzed. We analyzed the decrease or lack of decrease in the percentage of Ki67-positive cells between core biopsy samples and surgical specimens and correlated this value with outcome.

Results
Twenty-five percent of patients presented with luminal A-like tumors, 45% had luminal B-like tumors, 14% had triple-negative BC, 5% had HER2-positive BC, and 11% had triple-positive BC. Patients were predominantly diagnosed with stage III disease (52%) and high-grade tumors (46%). Median Ki67 level was 20% before NAC, which decreased to a median of 10% after NAC. Fifty-seven percent of patients had a decrease in Ki67 percentage. Ki67 decrease significantly correlated with better DFS and OS compared with no decrease, particularly in the luminal B subgroup. Multivariate analysis showed that nonreduction of Ki67 significantly increased the hazard ratio of recurrence and death by 3.39 (95% confidence interval [CI] 1.8–6.37) and 7.03 (95% CI 2.6–18.7), respectively.

Conclusion
Patients without a decrease in Ki67 in residual tumors after NAC have poor prognosis. This warrants the introduction of new therapeutic strategies in this setting.

Implications for Practice
This study evaluates the change in Ki67 percentage before and after neoadjuvant chemotherapy (NAC) and its relationship with survival outcomes in patients with breast cancer who did not achieve complete pathological response (pCR). These patients, a heterogeneous group with diverse prognoses that cannot be treated using a single algorithm, pose a challenge to clinicians. This study identified a subgroup of these patients with a poor prognosis, those with luminal B-like tumors without a Ki67 decrease after NAC, thus justifying the introduction of new therapeutic strategies for patients who already present a favorable prognosis (luminal B-like with Ki67 decrease).
Conhecimento de parada cardiorrespiratória dos profissionais de saúde em um hospital público: estudo transversal
Authors: Gonçalves de Oliveira Sarah Fernanda , Pereira Moreira Sandra Maria Belmonte , Vieira Liana Lima , Gardenghi Giulliano 
Published: February 2018

Introdução: A Parada Cardiorrespiratória é definida como a interrupção súbita da atividade mecânica ventricular útil e suficiente, e da respiração. O sucesso na reversão desse quadro depende da qualidade do atendimento que a equipe oferece e uma resposta rápida e hábil pode fazer a diferença entre mortalidade e ausência/instalação de sequelas. Objetivo: avaliar o conhecimento teórico de profissionais médicos e enfermeiros no diagnóstico e tratamento da parada cardiorrespiratória em adultos em um hospital público de Goiânia – GO. Métodos: Trata-se de um estudo observacional e descritivo. Aplicou-se questionário próprio específico contendo Perfil do profissional e Avaliação de Conhecimento Teórico sobre Parada Cardiorrespiratória e Ressuscitação Cardiopulmonar no adulto, fundamentado nas recomendações da American Heart Association de 2015. Resultados: De 147 participantes, 89,80% foram reprovados em seus conhecimentos e apenas 10,20% da amostra foi aprovada. Quando associado o padrão de aprovação com as categorias profissionais observou-se que 4,41% dos enfermeiros e 15,19% dos médicos foram aprovados com p=0,031. Conclusão: o estudo evidencia uma deficiência no conhecimento dos profissionais sobre manejo na Parada Cardiorrespiratória. Sugere-se padronização do atendimento e otimização do treinamento em serviço para que haja maior capacitação e atitude profissional, paralelamente ao progresso científico e tecnológico.
Relapse and Mortality Risk of Stage I Testicular Cancer
Authors: Florvall Cecilia , Frederiksen Peder , Lauritsen Jakob , Bandak Mikkel , Kier M Gry G , Mortensen Mette S , Kreiberg Michael , Daugaard Gedske 
Published: February 2018

Objectives. – To assess the medical insurance risk for patients with stage I testicular cancer (TC), by calculating the overall mortality risk with and without relapse, and compare it to men from the Danish population.
Background. – Testicular cancer is the most common malignancy in young males. Outcomes of a Danish cohort of 3366 patients with stage I TC (1366 non-seminomas (NSTC) and 2000 Seminomas (STC)), were analyzed.
Method. – The data were analyzed by the “illness-death” model. For the analysis of the transitions between diagnosis, relapse and death we adopted a parametric approach, where the relationship between the intensities and the effect of covariates were specified by Poisson regression models for NSTC and STC individually.
Results. – In the NSTC group, 422 patients relapsed. Six relapses (1.4%) occurred after 5 years of follow-up. In the STC group, 389 relapsed. The relapse rate after 5 years was 4.1%. The overall mortality analyses showed that the standardized mortality ratio (SMR) for men with NSTC without relapse, was slightly lower than in the matched general population of Danish men (SMR = 0.9). In STC patients without relapse, SMR was 0.80. Relapse raised the overall mortality by a factor 2.0 for NSTC and 1.5 for STC.
Conclusions. – The fact that few relapses occur 5 years after diagnosis is an important finding for risk assessment in life insurance. It makes it possible to insure men diagnosed with stage I TC, who have not experienced relapse 5 years after diagnosis, on normal terms.
Imigração de mulheres haitianas em Belo Horizonte/Brasil: identidades femininas, relatos de si e autonomia
Authors: Salgueiro Ângela Cristina , Therrier Dina 
Published: February 2018

O trabalho busca analisar como mulheres haitianas imigrantes elaboram “relatos de si” de modo a revelar, em entrevistas semi-estruturadas, suas existências e experiências na cidade de Belo Horizonte, destacando as práticas e ações intersubjetivas que constroem para viverem e ser valorizadas no cotidiano do país e no universo do trabalho. Este trabalho procurou mostrar, através das entrevistas, como mulheres haitianas imigrantes compreendem seu cotidiano e o trabalho que realizam no Brasil e as relações sociais que condicionam esse trabalho e constrangem sua ação, auto-realização e autonomia. Ganhou destaque o esforço dessas mulheres em conferir sentido à sua trajetória migratória e diaspórica, em reorganizar a narrativa da vida e de seu cotidiano, a invenção comunicacional e relacional de novas formas de existir, questionando o preconceito, a desvalorização e a falta de reconhecimento de suas capacidades e contribuições.
Gerakan damai ala kelompok-kelompok islamis di dunia Islam
Authors: Kuswaya Adang 
Published: February 2018

Judul buku yang diberikan penerbit Umat Bergerak Mobilisasi Damai Kaum Islamis di Indonesia, Malaysia, dan Turki. Sedangkan judul buku yang diberikan penulis Peaceful Islamist Mobilization in The Muslim World. Sepintas penulis berfikir bahwa judul penulisapenerjemahan ke dalam bahasa Indonesia tersebut dilakukan supaya lebih memikat perhatiadari pembaca. Untuk itu, penulis memilih judul review untuk buku ini dengan Gerakan damai ala kelompok-kelompok islamis di dunia Islam.
POTENCIALIDADES E LIMITA��ES APONTADAS PELOS ACAD�MICOS AO AVALIAR O CURSO DE LICENCIATURA EM QU�MICA EM EAD DO IFMT
Authors: Le�o Marcelo Franco , Assis Aline Silva de , Oliveira Eniz Concei��o , Del Pino Jos� Claudio 
Published: February 2018

RESUMO
O presente estudo teve como objetivo analisar as percep��es dos acad�micos sobre o andamento do curso de Licenciatura em Qu�mica, ofertado na modalidade da Educa��o a Dist�ncia (EaD), pelo Instituto Federal de Mato Grosso Campus Cuiab�-Bela Vista. Realizado durante o primeiro semestre de 2017, esta pesquisa caracteriza-se como levantamento descritivo e explorat�rio, cuja abordagem � qualitativa. Dos 361 acad�micos matriculados no curso neste per�odo, participaram voluntariamente da pesquisa 57 deles. O instrumento utilizado para coletar dados foi um formul�rio eletr�nico, via Google Drive, contendo quatro quest�es abertas. As informa��es coletadas foram analisadas por meio da t�cnica an�lise de conte�do. As limita��es/dificuldades encontradas no decorrer do curso foram: compreender conceitos, falta de comunica��o com professores e tutores de forma online e falta de momentos presenciais. J� como potencialidades, os acad�micos citaram: a flexibilidade de hor�rio, a acessibilidade, a autonomia e o incentivo a pesquisa. A identifica��o de limita��es e potencialidades deste curso poder� contribuir para que outros cursos de licenciatura em qu�mica em EaD avaliem seus desempenhos, utilizem aspectos que deram certo e evitem os n�o favor�veis. Estes aspectos positivos e negativos relacionados a cursos em EaD precisam ser discutidos e aprofundados por gestores e professores formadores dessa modalidade de ensino.
Palavras-chave: Avalia��o; educa��o a dist�ncia; forma��o de professores.
METODOLOGIAS E RECURSOS UTILIZADOS EM AULAS PARA ENSINAR QU�MICA: ESTADO DA ARTE EM ATAS DO ENPEC (2007-2015)
Authors: Almeida Meliana Silva de , Le�o Marcelo Franco 
Published: February 2018

RESUMO
Na Contemporaneidade, saber como ensinar Qu�mica e para que serve este estudo � t�o importante quanto saber o que se ensinar, ou seja, os conceitos da �rea. O presente estudo teve como objetivo realizar um levantamento das metodologias de ensino empregadas em aulas de Qu�mica, cujas experi�ncias pedag�gicas foram publicadas nas �ltimas cinco atas do Encontro Nacional de Pesquisa em Educa��o em Ci�ncias (ENPEC). De car�ter descritivo e explorat�rio, essa pesquisa b�sica caracteriza-se como um levantamento tipo estado da arte, cuja abordagem � mista. Para coletar dados, a an�lise bibliogr�fica foi necess�ria, pois os objetos do estudo foram os trabalhos publicados nas atas desse evento. Ao todo, foram encontrados 67 trabalhos envolvendo diversas metodologias de ensino e recursos did�ticos para aulas de qu�mica, sendo as mais incidentes: as pr�ticas experimentais com materiais alternativos, o uso de softwares educacionais, a elabora��o de mapas conceituais, o uso de jogos did�ticos como os de cartas, tabuleiro, jogos midi�ticos, aulas que utilizaram tecnologias digitais da informa��o e da comunica��o, entre outras. Os resultados demonstram a import�ncia da utiliza��o de metodologias diferenciadas em sala de aula e o quanto o ensino de Qu�mica pode ser favorecido de acordo com os trabalhos analisados.
Palavras-chave: Aulas Inovadoras; Ensino de qu�mica; Materiais alternativos.
DOC�NCIA E FORMA��O: HIST�RIAS DE VIDA DE ACAD�MICOS DE HIST�RIA
Authors: Cardoso Bruna Da Silva , J�nior Dernival Ven�ncio Ramos , Nakashima Ros�ria Helena Ruiz 
Published: February 2018

RESUMO
Este trabalho expressa os resultados e as reflex�es empreendidas em uma pesquisa de inicia��o cient�fica, realizada entre 2014 e 2015, sobre hist�rias de vida de acad�micos do curso de Licenciatura em Hist�ria da Universidade Federal do Tocantins (UFT), Campus de Aragua�na. Fizemos o uso da metodologia da Hist�ria Oral e analisamos nos relatos qual a rela��o doc�ncia e forma��o para os sujeitos da pesquisa, identificando o perfil dos professores em forma��o inicial. A partir das an�lises dos relatos, conclu�mos que a escolha pela licenciatura ocorreu pelas condi��es financeiras e sociais em que se encontravam e se encontram os acad�micos. Eles estabeleceram rela��es entre doc�ncia e forma��o e reconheceram a import�ncia da profiss�o docente e do curso de Hist�ria em suas vidas.
Palavras-chave: Hist�rias de vida; Doc�ncia; Forma��o Inicial.
Concepciones de los profesores acerca de la importancia del desarrollo de la competencia lingüística de los alumnos, para enseñar
Authors: Cabrera Alexandra 
Published: February 2018

"Para saber de ello visitó tres lugares, donde fue recibido con la generosa hospitalidad de los lugareños, con los que pasó muchas horas de conversación amena, escuchando sus relatos, atentamente, porque quería aprender. Le mostraron sus fotos y lo invitaron a sus casas, y supo más de ellos. Luego, hubo de detenerse porque era tiempo de escribir su propio relato, antes de que otras rutas o las de siempre lo absorbieran y lo aprendido quedara en el recuerdo. Quiso preservar el viaje del olvido". Su nuevo relato surgió del análisis de aquellos relatos, de la mirada cuidadosa, respetuosa de sus casas, de sus clases, y también de sus fotos, de sus planes de curso. Aparecieron, entonces, conclusiones que cerraron su propio texto. Aunque no fueron clausura, porque otros viajes emergieron de éste.
Enseñanza de la geometría en la escuela primaria. Cómo entrelaza el maestro, en sus prácticas, la matemática, el contexto y sus alumnos
Authors: Fripp Ariel 
Published: February 2018

En este trabajo se presentan las líneas centrales que articularon una investigación acerca de la construcción metodológica de las prácticas de enseñanza de la Geometría en la escuela primaria. Esa investigación se sustentó en una concepción positiva del trabajo de los docentes del nivel primario, tomando como hipótesis la convicción de que ellos reflexionan sobre sus prácticas; interesó buscar datos que iluminaran las maneras singulares en las que esos docentes construyen las prácticas de aula en el momento de enseñar Geometría intentando responder si esa construcción difiere o no según el objeto de estudio y el área de conocimiento al cual pertenece. Para ello, a través de entrevistas en cuatro colegios privados de la ciudad de Montevideo se pudo llegar a categorizar las prácticas de enseñanza geométrica, como prácticas de materialización, de utilidad, de problematización, de reiteración, de sobrevuelo y de responsabilidad social.