bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides
Authors: Koch Hanna , Dürwald Alexandra , Schweder Thomas , Noriega-Ortega Beatriz , Vidal-Melgosa Silvia , Hehemann Jan-Hendrik , Dittmar Thorsten , Freese Heike M , Becher Dörte , Simon Meinhard , Wietz Matthias 
Published: August 2018

Abstract
Algal polysaccharides are an important bacterial nutrient source and central component of marine food webs. However, cellular and ecological aspects concerning the bacterial degradation of polysaccharide mixtures, as presumably abundant in natural habitats, are poorly understood. Here, we contextualize marine polysaccharide mixtures and their bacterial utilization in several ways using the model bacterium Alteromonas macleodii 83-1, which can degrade multiple algal polysaccharides and contributes to polysaccharide degradation in the oceans. Transcriptomic, proteomic and exometabolomic profiling revealed cellular adaptations of A. macleodii 83-1 when degrading a mix of laminarin, alginate and pectin. Strain 83-1 exhibited substrate prioritization driven by catabolite repression, with initial laminarin utilization followed by simultaneous alginate/pectin utilization. This biphasic phenotype coincided with pronounced shifts in gene expression, protein abundance and metabolite secretion, mainly involving CAZymes/polysaccharide utilization loci but also other functional traits. Distinct temporal changes in exometabolome composition, including the alginate/pectin-specific secretion of pyrroloquinoline quinone, suggest that substrate-dependent adaptations influence chemical interactions within the community. The ecological relevance of cellular adaptations was underlined by molecular evidence that common marine macroalgae, in particular Saccharina and Fucus, release mixtures of alginate and pectin-like rhamnogalacturonan. Moreover, CAZyme microdiversity and the genomic predisposition towards polysaccharide mixtures among Alteromonas spp. suggest polysaccharide-related traits as an ecophysiological factor, potentially relating to distinct ‘carbohydrate utilization types’ with different ecological strategies. Considering the substantial primary productivity of algae on global scales, these insights contribute to the understanding of bacteria–algae interactions and the remineralization of chemically diverse polysaccharide pools, a key step in marine carbon cycling.
Diverse diazotrophs are present on sinking particles in the North Pacific Subtropical Gyre
Authors: Farnelid Hanna , Turk-Kubo Kendra , Ploug Helle , Ossolinski Justin E , Collins James R , Van Mooy Benjamin A S , Zehr Jonathan P 
Published: August 2018

Abstract
Sinking particles transport carbon and nutrients from the surface ocean into the deep sea and are considered hot spots for bacterial diversity and activity. In the oligotrophic oceans, nitrogen (N2)-fixing organisms (diazotrophs) are an important source of new N but the extent to which these organisms are present and exported on sinking particles is not well known. Sinking particles were collected every 6 h over a 2-day period using net traps deployed at 150 m in the North Pacific Subtropical Gyre. The bacterial community and composition of diazotrophs associated with individual and bulk sinking particles was assessed using 16S rRNA and nifH gene amplicon sequencing. The bacterial community composition in bulk particles remained remarkably consistent throughout time and space while large variations of individually picked particles were observed. This difference suggests that unique biogeochemical conditions within individual particles may offer distinct ecological niches for specialized bacterial taxa. Compared to surrounding seawater, particle samples were enriched in different size classes of globally significant N2-fixing cyanobacteria including Trichodesmium, symbionts of diatoms, and the unicellular cyanobacteria Crocosphaera and UCYN-A. The particles also contained nifH gene sequences of diverse non-cyanobacterial diazotrophs suggesting that particles could be loci for N2 fixation by heterotrophic bacteria. The results demonstrate that diverse diazotrophs were present on particles and that new N may thereby be directly exported from surface waters on sinking particles.
Genetic diversity and phenotypic plasticity of AHL-mediated Quorum sensing in environmental strains of Vibrio mediterranei
Authors: Girard Léa , Lantoine François , Lami Raphaël , Vouvé Florence , Suzuki Marcelino T , Baudart Julia 
Published: August 2018

Abstract
N-Acyl homoserine lactone (AHL)-mediated Quorum sensing (QS) is one of the most studied social behavior among Proteobacteria. However, despite the current knowledge on QS-associated phenotypes such as bioluminescence, biofilm formation, or pathogenesis, the characterization of environmental factors driving QS in realistic ecological settings remains scarce. We investigated the dynamics of AHL and AHL-producing Vibrio among 840 isolates collected fortnightly from the Salses-Leucate Mediterranean lagoon in spring and summer 2015 and 2016. Vibrio isolates were characterized by gyrB gene sequencing, Enterobacterial repetitive intergenic consensus polymerase chain reaction, and genome sequencing, and AHL production was investigated by a biosensors-based UHPLC–HRMS/MS approach. Our results revealed, for the first time, a succession of V. mediterranei isolates with different AHL production phenotypes over time and this dynamics was observed in a single genotype (average genomic nucleotide identity &gt;99.9). A multivariate DistLM analysis revealed that 83.4% of the temporal variation of V. mediterranei QS phenotypes was explained by environmental variables. Overall, our results suggest that isolates of a single genotype are able to change their QS phenotypes in response to environmental conditions, highlighting the phenotypic plasticity of bacterial communication in the environment.
Effect of valproate and pregabalin on human anxiety-like behaviour in a randomised controlled trial
Authors: Bach Dominik R. , Korn Christoph W. , Vunder Johanna , Bantel Antonia 
Published: August 2018

AbstractValproate is an anticonvulsant drug with strong preclinical evidence for reducing anxiety behaviour in rodents but no clear clinical evidence. To motivate clinical trials, we here investigate the use of valproate in a translational human model of anxiety behaviour. In a double-blind, randomised, placebo-controlled trial, n = 118 healthy participants played a previously validated approach/avoidance conflict computer game to measure anxiety-like behaviour, while under 400 mg valproate, under 200 mg of the established anxiolytic/anticonvulsant pregabalin, or under placebo. Saccadic peak velocity and subjective ratings were assessed to control for drug-induced sedation. Compared to placebo, valproate and pregabaline were anxiolytic in the primary outcome, and several secondary outcomes. Bayesian model comparison decisively demonstrated no differences between the two drugs. Subjective and objective sedation was significantly more pronounced under pregabalin than valproate, but did not explain anxiolytic effects. We demonstrate acute anxiolytic properties of valproate in healthy humans. Both drugs have similar anxiolytic properties at the doses used. Valproate is less sedative than pregabalin. Our results suggest clinical trials on the use of valproate in anxiolytic treatment. More generally, we propose a strategy of screening drugs in human preclinical models that can directly be compared across species, such as the approach/avoidance conflict computer game used here. This approach could thus facilitate translational anxiety research.
Neural hypersensitivity to pleasant touch in women remitted from anorexia nervosa
Authors: Bischoff-Grethe Amanda , Wierenga Christina E. , Berner Laura A. , Simmons Alan N. , Bailer Ursula , Paulus Martin P. , Kaye Walter H. 
Published: August 2018

AbstractInteroception, or the sensing and integration of bodily state signals, has been implicated in anorexia nervosa (AN), given that the hallmark symptoms involve food restriction and body image disturbance. Here we focus on brain response to the anticipation and experience of affective interoceptive stimuli. Women remitted from AN (RAN;N = 18) and healthy comparison women (CW;N = 26) underwent a pleasant affective touch paradigm consisting of gentle strokes with a soft brush administered to the forearm or palm during functional neuroimaging. RAN had a lower brain response relative to CW during anticipation of touch, but a greater response when experiencing touch in the right ventral mid-insula. In RAN, this reduced anticipatory response was associated with higher levels of harm avoidance. Exploratory analyses in RAN also suggested that lower response during touch anticipation was associated with greater body dissatisfaction and higher perceived touch intensity ratings. This reduced responsivity to the anticipation of pleasant affective interoceptive stimuli in association with higher harm avoidance, along with an elevated response to the experience of touch, suggests an impaired ability in AN to predict and interpret incoming physiological stimuli. Impaired interoception may thus impact one’s sense of self, thereby supporting observations of disturbed body image and avoidance of affective and social stimuli. Therapeutic approaches that help AN to better anticipate and interpret salient affective stimuli or improve tolerance of interoceptive experiences may be an important addition to current interventions.
Genomic patterns of progression in smoldering multiple myeloma
Authors: Bolli Niccolò , Maura Francesco , Minvielle Stephane , Gloznik Dominik , Szalat Raphael , Fullam Anthony , Martincorena Inigo , Dawson Kevin J. , Samur Mehmet Kemal , Zamora Jorge , Tarpey Patrick , Davies Helen , Fulciniti Mariateresa , Shammas Masood A. , Tai Yu Tzu , Magrangeas Florence , Moreau Philippe , Corradini Paolo , Anderson Kenneth , Alexandrov Ludmil , Wedge David C. , Avet-Loiseau Herve , Campbell Peter , Munshi Nikhil 
Published: August 2018

AbstractWe analyzed whole genomes of unique paired samples from smoldering multiple myeloma (SMM) patients progressing to multiple myeloma (MM). We report that the genomic landscape, including mutational profile and structural rearrangements at the smoldering stage is very similar to MM. Paired sample analysis shows two different patterns of progression: a “static progression model”, where the subclonal architecture is retained as the disease progressed to MM suggesting that progression solely reflects the time needed to accumulate a sufficient disease burden; and a “spontaneous evolution model”, where a change in the subclonal composition is observed. We also observe that activation-induced cytidine deaminase plays a major role in shaping the mutational landscape of early subclinical phases, while progression is driven by APOBEC cytidine deaminases. These results provide a unique insight into myelomagenesis with potential implications for the definition of smoldering disease and timing of treatment initiation.
Nucleoside-modified mRNA immunization elicits influenza virus hemagglutinin stalk-specific antibodies
Authors: Pardi Norbert , Parkhouse Kaela , Kirkpatrick Ericka , McMahon Meagan , Zost Seth J. , Mui Barbara L. , Tam Ying K. , Karikó Katalin , Barbosa Christopher J. , Madden Thomas D. , Hope Michael J. , Krammer Florian , Hensley Scott E. , Weissman Drew 
Published: August 2018

AbstractCurrently available influenza virus vaccines have inadequate effectiveness and are reformulated annually due to viral antigenic drift. Thus, development of a vaccine that confers long-term protective immunity against antigenically distant influenza virus strains is urgently needed. The highly conserved influenza virus hemagglutinin (HA) stalk represents one of the potential targets of broadly protective/universal influenza virus vaccines. Here, we evaluate a potent broadly protective influenza virus vaccine candidate that uses nucleoside-modified and purified mRNA encoding full-length influenza virus HA formulated in lipid nanoparticles (LNPs). We demonstrate that immunization with HA mRNA-LNPs induces antibody responses against the HA stalk domain of influenza virus in mice, rabbits, and ferrets. The HA stalk-specific antibody response is associated with protection from homologous, heterologous, and heterosubtypic influenza virus infection in mice.
Hapten-mediated recruitment of polyclonal antibodies to tumors engenders antitumor immunity
Authors: Schrand Brett , Clark Emily , Levay Agata , Capote Ailem Rabasa , Martinez Olivier , Brenneman Randall , Castro Iris , Gilboa Eli 
Published: August 2018

AbstractUptake of tumor antigens by tumor-infiltrating dendritic cells is limiting step in the induction of tumor immunity, which can be mediated through Fc receptor (FcR) triggering by antibody-coated tumor cells. Here we describe an approach to potentiate tumor immunity whereby hapten-specific polyclonal antibodies are recruited to tumors by coating tumor cells with the hapten. Vaccination of mice against dinitrophenol (DNP) followed by systemic administration of DNP targeted to tumors by conjugation to a VEGF or osteopontin aptamer elicits potent FcR dependent, T cell mediated, antitumor immunity. Recruitment of αGal-specific antibodies, the most abundant naturally occurring antibodies in human serum, inhibits tumor growth in mice treated with a VEGF aptamer–αGal hapten conjugate, and recruits antibodies from human serum to human tumor biopsies of distinct origin. Thus, treatment with αGal hapten conjugated to broad-spectrum tumor targeting ligands could enhance the susceptibility of a broad range of tumors to immune elimination.
One-pot aminobenzylation of aldehydes with toluenes
Authors: Wang Zhiting , Zheng Zhipeng , Xu Xinyu , Mao Jianyou , Walsh Patrick J. 
Published: August 2018

AbstractAmines are fundamental motifs in bioactive natural products and pharmaceuticals. Using simple toluene derivatives, a one-pot aminobenzylation of aldehydes is introduced that provides rapid access to amines. Simply combining benzaldehydes, toluenes, NaN(SiMe3)2, and additive Cs(O2CCF3) (0.35 equiv.) generates a diverse array of 1,2-diarylethylamine derivatives (36 examples, 56–98% yield). Furthermore, suitably functionalized 1,2-diarylethylamines were transformed into 2-aryl-substituted indoline derivatives via Buchwald–Hartwig amination. It is proposed that the successful deprotonation of toluene by MN(SiMe3)2 is facilitated by cation–π interactions between the arene and the group(I) cation that acidify the benzylic C–Hs.
Precession-band variance missing from East Asian monsoon runoff
Authors: Clemens S. C. , Holbourn A. , Kubota Y. , Lee K. E. , Liu Z. , Chen G. , Nelson A. , Fox-Kemper B. 
Published: August 2018

AbstractSpeleothem CaCO3 δ18O is a commonly employed paleomonsoon proxy. However, inferring local rainfall amount from speleothem δ18O can be complicated due to changing source water δ18O, temperature effects, and rainout over the moisture transport path. These complications are addressed using δ18O of planktonic foraminiferal CaCO3, offshore from the Yangtze River Valley (YRV). The advantage is that the effects of global seawater δ18O and local temperature changes can be quantitatively removed, yielding a record of local seawater δ18O, a proxy that responds primarily to dilution by local precipitation and runoff. Whereas YRV speleothem δ18O is dominated by precession-band (23 ky) cyclicity, local seawater δ18O is dominated by eccentricity (100 ky) and obliquity (41 ky) cycles, with almost no precession-scale variance. These results, consistent with records outside the YRV, suggest that East Asian monsoon rainfall is more sensitive to greenhouse gas and high-latitude ice sheet forcing than to direct insolation forcing.
V2a interneuron diversity tailors spinal circuit organization to control the vigor of locomotor movements
Authors: Song Jianren , Dahlberg Elin , El Manira Abdeljabbar 
Published: August 2018

AbstractLocomotion is a complex motor task generated by spinal circuits driving motoneurons in a precise sequence to control the timing and vigor of movements, but the underlying circuit logic remains to be understood. Here we reveal, in adult zebrafish, how the diversity and selective distribution of two V2a interneuron types within the locomotor network transform commands into an appropriate, task-dependent circuit organization. Bursting-type V2a interneurons with unidirectional axons predominantly target distal dendrites of slow motoneurons to provide potent, non-linear excitation involving NMDA-dependent potentiation. A second type, non-bursting V2a interneurons with bidirectional axons, predominantly target somata of fast motoneurons, providing weaker, non-potentiating excitation. Together, this ensures the rapid, first-order recruitment of the slow circuit, while reserving the fast circuit for highly salient stimuli involving synchronous inputs. Our results thus identify how interneuron diversity is captured and transformed into a parsimonious task-specific circuit design controlling the vigor of locomotion.
Experience-dependent structural plasticity targets dynamic filopodia in regulating dendrite maturation and synaptogenesis
Authors: Sheng Chengyu , Javed Uzma , Gibbs Mary , Long Caixia , Yin Jun , Qin Bo , Yuan Quan 
Published: August 2018

AbstractHighly motile dendritic protrusions are hallmarks of developing neurons. These exploratory filopodia sample the environment and initiate contacts with potential synaptic partners. To understand the role for dynamic filopodia in dendrite morphogenesis and experience-dependent structural plasticity, we analyzed dendrite dynamics, synapse formation, and dendrite volume expansion in developing ventral lateral neurons (LNvs) of the Drosophila larval visual circuit. Our findings reveal the temporal coordination between heightened dendrite dynamics with synaptogenesis in LNvs and illustrate the strong influence imposed by sensory experience on the prevalence of dendritic filopodia, which regulate the formation of synapses and the expansion of dendritic arbors. Using genetic analyses, we further identified Amphiphysin (Amph), a BAR (Bin/Amphiphysin/Rvs) domain-containing protein as a required component for tuning the dynamic state of LNv dendrites and promoting dendrite maturation. Taken together, our study establishes dynamic filopodia as the key cellular target for experience-dependent regulation of dendrite development.
Entropy contributions to phase stability in binary random solid solutions
Authors: Manzoor Anus , Pandey Shubham , Chakraborty Debajit , Phillpot Simon R. , Aidhy Dilpuneet S. 
Published: August 2018

AbstractHigh entropy alloys contain multiple elements in large proportions that make them prone to phase separation. These alloys generally have shallow enthalpy of mixing which makes the entropy contributions of similar magnitude. As a result, the phase stability of these alloys is equally dependent on enthalpy and entropy of mixing and understanding the individual contribution of thermodynamic properties is critical. In the overall vision of designing high entropy alloys, in this work, using density functional theory calculations, we elucidate the contributions of various entropies, i.e., vibrational, electronic and configurational towards the phase stability of binary alloys. We show that the contribution of electronic entropy is very small compared to the vibrational and configurational entropies, and does not play a significant role in the phase stability of alloys. The configurational and vibrational entropies can either destabilize or can collectively contribute to stabilize the solid solutions. As a result, even those systems that have negative mixing enthalpy can show phase instability, revealed as a miscibility gap; conversely, systems with positive mixing enthalpy can be phase stable due to entropic contributions. We suggest that including entropic contributions are critical in the development of theoretical framework for the computational prediction of stable, single-phase high entropy alloys that have shallow mixing enthalpies, unlike ordered intermetallics.
1q21.1 microduplication: large verbal–nonverbal performance discrepancy and ddPCR assays of HYDIN/HYDIN2 copy number
Authors: Xavier Jean , Zhou Bo , Bilan Frédéric , Zhang Xianglong , Gilbert-Dussardier Brigitte , Viaux-Savelon Sylvie , Pattni Reenal , Ho Steve S. , Cohen David , Levinson Douglas F. , Urban Alexander E. , Laurent-Levinson Claudine 
Published: August 2018

AbstractMicroduplication of chromosome 1q21.1 is observed in ~0.03% of adults. It has a highly variable, incompletely penetrant phenotype that can include intellectual disability, global developmental delay, specific learning disabilities, autism, schizophrenia, heart anomalies and dysmorphic features. We evaluated a 10-year-old-male with a 1q21.1 duplication by CGH microarray. He presented with major attention deficits, phonological dysphasia, poor fine motor skills, dysmorphia and mild autistic features, but not the typical macrocephaly. Neuropsychiatric evaluation demonstrated a novel phenotype: an unusually large discrepancy between non-verbal capacities (borderline-impaired WISC-IV index scores of 70 for Working Memory and 68 for Processing Speed) vs. strong verbal skills – scores of 126 for Verbal Comprehension (superior) and 111 for Perceptual Reasoning (normal). HYDIN2 has been hypothesized to underlie macrocephaly and perhaps cognitive deficits in this syndrome, but assessment of HYDIN2 copy number by microarray is difficult because of extensive segmental duplications. We performed whole-genome sequencing which supported HYDIN2 duplication (chr1:146,370,001-148,590,000, 2.22 Mb, hg38). To evaluate copy number more rigorously we developed droplet digital PCR assays of HYDIN2 (targeting unique 1 kb and 6 kb insertions) and its paralog HYDIN (targeting a unique 154 bp segment outside the HYDIN2 overlap). In an independent cohort, ddPCR was concordant with previous microarray data. Duplication of HYDIN2 was confirmed in the patient by ddPCR. This case demonstrates that a large discrepancy of verbal and non-verbal abilities can occur in 1q21.1 duplication syndrome, but it remains unclear whether this has a specific genomic basis. These ddPCR assays may be useful for future research on HYDIN2 copy number.
Compliant plant wearables for localized microclimate and plant growth monitoring
Authors: Nassar Joanna M. , Khan Sherjeel M. , Villalva Diego Rosas , Nour Maha M. , Almuslem Amani S. , Hussain Muhammad M. 
Published: August 2018

AbstractThe microclimate surrounding a plant has major effect on its health and photosynthesis process, where certain plants struggle in suboptimal environmental conditions and unbalanced levels of humidity and temperature. The ability to remotely track and correlate the effect of local environmental conditions on the healthy growth of plants can have great impact for increasing survival rate of plants and augmenting agriculture output. This necessitates the widespread distribution of lightweight sensory devices on the surface of each plant. Using flexible and biocompatible materials coupled with a smart compact design for a low power and lightweight system, we develop widely deployed, autonomous, and compliant wearables for plants. The demonstrated wearables integrate temperature, humidity and strain sensors, and can be intimately deployed on the soft surface of any plant to remotely and continuously evaluate optimal growth settings. This is enabled through simultaneous detection of environmental conditions while quantitatively tracking the growth rate (viz. elongation). Finally, we establish a nature-inspired origami-assembled 3D-printed “PlantCopter”, used as a launching platform for our plant wearable to enable widespread microclimate monitoring in large fields.