bluuebunny/crossref_metadata_2025_split huggingface dataset:
A nova legislação ambiental brasileira e seus efeitos sobre a reestruturação de nascentes e remanescentes florestais
Authors: Gomes Emerson Chaves Ferreira , Jesus Edilma Nunes de , Oliveira Natália Nogueira de , Gonçalves Júnior Luiz , Cabral Felipe Gonçalves dos Santos , Resende Marisa Seoane Rio 
Published: September 2018

Foi conduzido um trabalho de diagnóstico ambiental da bacia hidrográfica do Córrego Saraiva, situado no Município de Betim, MG, pela identificação e caracterização das principais nascentes e remanescentes florestais. Além da quantificação da área total dos fragmentos florestais, foi calculada a área passível de recuperação ambiental de ambientes ciliares comparando a legislação ambiental brasileira representada pela Lei Federal nº 4.771, de 15 de setembro de 1965 com a legislação vigente, Lei Federal nº 12.651, de 25 de maio de 2012. Os fragmentos da bacia foram avaliados por métricas de paisagem em Sistema de Informações Geográficas, com base em indicadores ecológicos: índice de circularidade; índice de isolamento; total de área de borda e tamanho médio das manchas. O estado de conservação das nascentes e dos remanescentes florestais na bacia hidrográfica do Córrego Saraiva é crítico. A legislação ambiental brasileira vigente requer menor área total a ser recuperada, comprometendo as funções ecológicas desempenhadas pelos ecossistemas ribeirinhos.
Effect of NSAIDs on Bone Healing Rates: A Meta-analysis
Authors: Wheatley Benjamin M. , Nappo Kyle E. , Christensen Daniel L. , Holman Ann M. , Brooks Daniel I. , Potter Benjamin K. 
Published: September 2018

Introduction:
NSAIDs inhibit osteogenesis and may result in delayed union or nonunion. The purpose of this meta-analysis was to determine whether their use leads to delayed union or nonunion.

Methods:
We systematically reviewed the literature reporting the effect of NSAIDs on bone healing. We included studies of pediatric and adult patients NSAID exposure and healing bone. The outcomes of interest were delayed union, nonunion, or pseudarthrosis with at least six months of follow-up. A maximum likelihood random-effects model was used to conduct meta-analysis and meta-regression.

Results:
NSAID exposure increased delayed union or nonunion (odds ratio [OR], 2.07; confidence interval [CI], 1.19 to 3.61). No effect was noted in pediatrics (OR, 0.58; CI, 0.27 to 1.21) or low dose/short duration of exposure (OR, 1.68; CI, 0.63 to 4.46).

Conclusion:
Analysis of the literature indicates a negative effect of NSAIDs on bone healing. In pediatric patients, NSAIDs did not have a significant effect. The effect may be dose or time dependent because low-dose/short-duration exposure did not affect union rates.
Evaluation of the 3M™ Molecular Detection Assay (MDA) 2 – Cronobacter for the Detection of Cronobacter species in Select Foods and Environmental Surfaces: Collaborative Study, First Action 2018.01
Authors: Bird Patrick , Benzinger M Joseph , Bastin Ben , Crowley Erin , Agin James , Goins David , Monteroso Lisa ,   Collaborators:, Abramovic T , Achen M , Budge E , Clemens N , Cruz A , Cuellar J , Diaz C , Hong Y , Ishii S , Liska C , Luquin C , Ma L , Martin K , Masanz G , Matijevic M , McKelvey P , Miller J , Molina S , Mustafic A , Podoreski I , Ricki I , Schindler B , Scollon A , Somoza C , Tinajero C , Thompson L , Wheeler S , Winslow A , Xu W 
Published: September 2018

Abstract
The 3M™ Molecular Detection Assay (MDA) 2 – Cronobacter combines the use of loop-mediated isothermal amplification to rapidly amplify nucleic acid sequences while using bioluminescence to detect the amplification. Using a paired study design, the MDA 2 – Cronobacter was compared with ISO 22964:2017 for the detection of Cronobacter species in powdered infant formula containing probiotics. Technicians from 11 laboratories from the United States, Mexico, and Croatia participated. Collaborators received test portions with three levels of contamination. Statistical analysis was conducted according to the probability of detection (POD) statistical model. Results obtained for the low-inoculum level test portions produced a difference in POD values obtained from combining all valid collaborator POD data values with 95% confidence intervals of –0.01, –0.12, and 0.10, indicating that the difference between methods was not statistically significant at the 0.05 probability level.
Le point de vente physique, entre espace de showrooming et espace d’achat
Authors: Heitz-Spahn Sandrine , Belaud Lydie , Yildiz Hélène 
Published: September 2018

Que peut faire une enseigne de distribution pour retenir le consommateur qui se trouve en magasin et qui a la possibilité de le quitter pour acheter le produit ailleurs, notamment sur Internet ? Cette recherche vise à identifier les facteurs influençant les arbitrages opérés par les consommateurs en matière de canal d’achat lorsqu’ils se trouvent en magasin. La théorie des niveaux de représentation de Liberman et Trope (1998) permet de montrer que la manière de représenter la situation d’achat influence le choix du canal d’achat. Le paradigme Personne-Objet-Situation (Punj et Stewart, 1983) donne un éclairage sur les facteurs situationnels qui peuvent impacter le choix de canal d’achat. Les résultats de l’étude expérimentale et de la série d’entretiens individuels montrent que l’urgence de l’achat et l’écart de prix entre Internet et magasin viennent modérer la relation entre le niveau de représentation de la situation et l’intention de quitter ou non le magasin. Des pistes de réflexion sont proposées aux enseignes de distribution sur les leviers prix et urgence perçue de l’achat.
La livraison collaborative des consommateurs connectés, nouvel enjeu de la logistique urbaine
Authors: Moncef Btissam , Akeb Hakim , Fourot Jean-Charles , Durand Bruno 
Published: September 2018

Cet article s’intéresse à la logistique collaborative dans le cadre de la livraison de colis aux particuliers dans les zones urbaines. Il vise à étudier la crowd logistics comme solution qui permet de réceptionner et re-livrer les colis en cas d’échec de livraison à domicile. À travers une modélisation suivie d’une simulation appliquée à la ville de Paris, nos résultats posent les bases de la mise en place d’un réseau de particuliers, appelé « voisinsrelais.com », capables de réceptionner les colis moyennant une rémunération. Ce travail sensibilise les distributeurs et les prestataires de services logistiques à ce nouveau mode de livraison qui permet d’éviter d’une part l’insatisfaction des clients et, d’autre part, des coûts supplémentaires et un impact environnemental négatif associés à une relivraison.
Identification of functionally distinct fibro-inflammatory and adipogenic stromal subpopulations in visceral adipose tissue of adult mice
Authors: Hepler Chelsea , Shan Bo , Zhang Qianbin , Henry Gervaise H , Shao Mengle , Vishvanath Lavanya , Ghaben Alexandra L , Mobley Angela B , Strand Douglas , Hon Gary C , Gupta Rana K 
Published: September 2018

White adipose tissue (WAT) remodeling is dictated by coordinated interactions between adipocytes and resident stromal-vascular cells; however, the functional heterogeneity of adipose stromal cells has remained unresolved. We combined single-cell RNA-sequencing and FACS to identify and isolate functionally distinct subpopulations of PDGFRβ+ stromal cells within visceral WAT of adult mice. LY6C- CD9- PDGFRβ+ cells represent highly adipogenic visceral adipocyte precursor cells (‘APCs’), whereas LY6C+ PDGFRβ+ cells represent fibro-inflammatory progenitors (‘FIPs’). FIPs lack adipogenic capacity, display pro-fibrogenic/pro-inflammatory phenotypes, and can exert an anti-adipogenic effect on APCs. The pro-inflammatory phenotype of PDGFRβ+ cells is regulated, at least in part, by NR4A nuclear receptors. These data highlight the functional heterogeneity of visceral WAT perivascular cells, and provide insight into potential cell-cell interactions impacting adipogenesis and inflammation. These improved strategies to isolate FIPs and APCs from visceral WAT will facilitate the study of physiological WAT remodeling and mechanisms leading to metabolic dysfunction.Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (<xref ref-type="decision-letter" rid="SA1">see decision letter</xref>).
Molecular analysis of oral microflora in patients with primary Sjögren’s syndrome by using high-throughput sequencing
Authors: Zhou Zhifang , Ling Guanghui , Ding Ning , Xun Zhe , Zhu Ce , Hua Hong , Chen Xiaochi 
Published: September 2018

BackgroundThe objective of this study was to characterize the oral microflora profile of primary Sjögren’s syndrome (pSS) patients, thereby revealing the connection between oral bacterial composition and dental caries, and to identify the “core microbiome” in the oral cavities of pSS patients and systemic healthy individuals by using a high-throughput sequencing technique.MethodsTwenty-two pSS patients and 23 healthy controls were enrolled in this study. Their clinical data and oral rinse samples were collected. The V3–V4 hypervariable regions of the bacterial 16S rRNA gene of samples were amplified and analyzed by high-throughput sequencing on the Illumina Miseq PE300 platform.ResultsBoth two groups were age- and sex-matched. There were significantly higher decayed, missing and filled teeth (DMFT) and decayed, missing and filled surfaces (DMFS) in the pSS group than in the control group (p < 0.01). Alpha diversity was depleted in pSS patients, compared with healthy controls (p < 0.01), while beta diversity between the two groups was not significantly different. Seven discriminative genera (LDA > 4) were found between the two groups in LEfSe (LDA Effect Size) analysis. The relative abundance ofVeillonellain pSS patients was fourfold higher, whileActinomyces,Haemophilus,Neisseria,Rothia,PorphyromonasandPeptostreptococcuswere significantly lower in pSS patients than in healthy controls. However, the correlation betweenVeillonellaand DMFT/DMFS was not significant (p > 0.05). In Venn diagram analysis, nine genera shared by all samples of two groups, which comprised 71.88% and 67.64% in pSS patients and controls, respectively.DiscussionThese findings indicate a microbial dysbiosis in pSS patients; notably,Veillonellamight be recognized as a biomarker in pSS patients. The core microbiome in pSS patients was similar to the systemic healthy population. These provide insight regarding advanced microbial prevention and treatment of severe dental caries in pSS patients. This study also provides basic data regarding microbiology in pSS.
Closing the gap: mixed stock analysis of three foraging populations of green turtles (Chelonia mydas) on the Great Barrier Reef
Authors: Jones Karina , Jensen Michael , Burgess Graham , Leonhardt Johanna , van Herwerden Lynne , Hazel Julia , Hamann Mark , Bell Ian , Ariel Ellen 
Published: September 2018

A solid understanding of the spatial ecology of green turtles (Chelonia mydas) is fundamental to their effective conservation. Yet this species, like many marine migratory species, is challenging to monitor and manage because they utilise a variety of habitats that span wide spatio-temporal scales. To further elucidate the connectivity between green turtle rookeries and foraging populations, we sequenced the mtDNA control region of 278 turtles across three foraging sites from the northern Great Barrier Reef (GBR) spanning more than 330 km: Cockle Bay, Green Island and Low Isles. This was performed with a newly developed assay, which targets a longer fragment of mtDNA than previous studies. We used a mixed stock analysis (MSA), which utilises genetic data to estimate the relative proportion of genetically distinct breeding populations found at a given foraging ground. Haplotype and nucleotide diversity was also assessed. A total of 35 haplotypes were identified across all sites, 13 of which had not been found previously in any rookery. The MSA showed that the northern GBR (nGBR), Coral Sea (CS), southern GBR (sGBR) and New Caledonia (NC) stocks supplied the bulk of the turtles at all three sites, with small contributions from other rookeries in the region. Stock contribution shifted gradually from north to south, although sGBR/CS stock dominated at all three sites. The major change in composition occured between Cockle Bay and Low Isles. Our findings, together with other recent studies in this field, show that stock composition shifts with latitude as a natural progression along a coastal gradient. This phenomenon is likely to be the result of ocean currents influencing both post-hatchling dispersal and subsequent juvenile recruitment to diverse coastal foraging sites.
Meta-analysis of northeast Atlantic marine taxa shows contrasting phylogeographic patterns following post-LGM expansions
Authors: Jenkins Tom L. , Castilho Rita , Stevens Jamie R. 
Published: September 2018

BackgroundComparative phylogeography enables the study of historical and evolutionary processes that have contributed to shaping patterns of contemporary genetic diversity across co-distributed species. In this study, we explored genetic structure and historical demography in a range of coastal marine species across the northeast Atlantic to assess whether there are commonalities in phylogeographic patterns across taxa and to evaluate whether the timings of population expansions were linked to the Last Glacial Maximum (LGM).MethodsA literature search was conducted using Web of Science. Search terms were chosen to maximise the inclusion of articles reporting on population structure and phylogeography from the northeast Atlantic; titles and abstracts were screened to identify suitable articles within the scope of this study. Given the proven utility of mtDNA in comparative phylogeography and the availability of these data in the public domain, a meta-analysis was conducted using published mtDNA gene sequences. A standardised methodology was implemented to ensure that the genealogy and demographic history of all mtDNA datasets were reanalysed in a consistent and directly comparable manner.ResultsMitochondrial DNA datasets were built for 21 species. The meta-analysis revealed significant population differentiation in 16 species and four main types of haplotype network were found, with haplotypes in some species unique to specific geographical locations. A signal of rapid expansion was detected in 16 species, whereas five species showed evidence of a stable population size. Corrected mutation rates indicated that the majority of expansions were estimated to have occurred after the earliest estimate for the LGM (∼26.5 Kyr), while few expansions were estimated to have pre-dated the LGM.ConclusionThis study suggests that post-LGM expansion appeared to be common in a range of marine taxa, supporting the concept of rapid expansions after the LGM as the ice sheets started to retreat. However, despite the commonality of expansion patterns in many of these taxa, phylogeographic patterns appear to differ in the species included in this study. This suggests that species-specific evolutionary processes, as well as historical events, have likely influenced the distribution of genetic diversity of marine taxa in the northeast Atlantic.
Modeled larval connectivity of a multi-species reef fish and invertebrate assemblage off the coast of Moloka‘i, Hawai‘i
Authors: Conklin Emily E. , Neuheimer Anna B. , Toonen Robert J. 
Published: September 2018

We use a novel individual-based model (IBM) to simulate larval dispersal around the island of Moloka‘i in the Hawaiian Archipelago. Our model uses ocean current output from the Massachusetts Institute of Technology general circulation model (MITgcm) as well as biological data on four invertebrate and seven fish species of management relevance to produce connectivity maps among sites around the island of Moloka‘i. These 11 species span the range of life history characteristics of Hawaiian coral reef species and show different spatial and temporal patterns of connectivity as a result. As expected, the longer the pelagic larval duration (PLD), the greater the proportion of larvae that disperse longer distances, but regardless of PLD (3–270 d) most successful dispersal occurs either over short distances within an island (<30 km) or to adjacent islands (50–125 km). Again, regardless of PLD, around the island of Moloka‘i, connectivity tends to be greatest among sites along the same coastline and exchange between northward, southward, eastward and westward-facing shores is limited. Using a graph-theoretic approach to visualize the data, we highlight that the eastern side of the island tends to show the greatest out-degree and betweenness centrality, which indicate important larval sources and connectivity pathways for the rest of the island. The marine protected area surrounding Kalaupapa National Historical Park emerges as a potential source for between-island larval connections, and the west coast of the Park is one of the few regions on Moloka‘i that acts as a net larval source across all species. Using this IBM and visualization approach reveals patterns of exchange between habitat regions and highlights critical larval sources and multi-generational pathways to indicate priority areas for marine resource managers.
ЭФФЕКТИВНОСТЬ ПРИМЕНЕНИЯ НАНОЧАСТИЦ СЕРЕБРА НА МОДЕЛИ КЛИНИЧЕСКИХ ИЗОЛЯТОВ ВОЗБУДИТЕЛЯ ТУБЕРКУЛЕЗА С РАЗЛИЧНОЙ ЛЕКАРСТВЕННОЙ УСТОЙЧИВОСТЬЮ, "Вестник Центрального научно-исследовательского института туберкулеза"
Authors: Захаров Андрей Владимирович , Хохлов Александр Леонидович 
Published: September 2018

В работе приводятся результаты исследования in vitro подавляющей активности наночастиц серебра на клинические изоляты МБТ с различной лекарственной устойчивостью возбудителя. В 1 группе исследовалось 117 изолятов МБТ с устойчивостью к изониазиду и другим препаратам, но чувствительностью к рифампицину, во 2 - 108 изолятов с МЛУ-МБТ. В 1 группе наиболее часто встречались спектры HSE (17,9%), HSKE (12,8%), HS (10,3%), во 2 - HRSE (8,3%), HRSK и HRSKE (по 7,4% каждый). Наиболее часто лекарственная устойчивость изолятов 1 группы наблюдалась к стрептомицину (80,3%), этамбутолу (59,8%), канамицину/амикацину (39,3%), протионамиду (21,4%), фторхинолонам (20,5%). Среди изолятов 2 группы максимальная устойчивость наблюдалась к стрептомицину (94,4%), канамицину/амикацину (56,5%), этамбутолу (52,8%), фторхинолонам (37,0%), протионамиду (34,3%). Всего было проведено 1350 бактериологических исследований. Лекарственная устойчивость возбудителя определялась методом абсолютных концентраций на плотных средах и методом пропорций на автоматическом анализаторе «Bactec MGIT 960», а также включала ПЦР-диагностику в анализаторе «GeneXpert». Изучалась подавляющая активность наночастиц серебра в изолированном варианте в концентрациях 5, 25, 50 мкг/мл. Так же исследовалась in vitro противотуберкулезная активность нанокомпозита, состоящего из наночастиц серебра в концентрациях 5, 25, 50 мкг/мл и противотуберкулезного препарата изониазида в концентрации 1 мкг/мл. Наночастицы размером 3-60 нм получали методом электрохимического растворения металла. Исследования показали, что наночастицы в растворе сохраняли стабильность при хранении в течение 300 суток. При смешивании растворов наночастиц серебра и изониазида, как показали исследования, наночастицы серебра не оказывали влияния на химическую стабильность и концентрацию изониазида в растворе при экспозиции 24 часа. Полное и значительное подавление роста МБТ (бактерицидное действие) наночастиц на изоляты 1 группы отмечалось в 48,1 ± 0,53% случаев, при этом наибольшая подавляющая активность отмечалась при концентрации наночастиц 5 мкг/мл - 58,1 ± 0,93%. При сочетании наночастиц с изониазидом прирост бактерицидного действия составил 17,2% (р 0,05). Бактерицидное действие изолированных наночастиц на клинические изоляты 2 группы с МЛУ составило 41,7%. Сочетанное использование наночастиц с изониазидом обеспечило прирост бактерицидного действия 18,8% (р 0,05). Полученные в эксперименте in vitro результаты свидетельствуют о потенцирующем действии наночастиц серебра на изониазид.
ЦЕНТРАЛЬНЫЙ НАУЧНО-ИССЛЕДОВАТЕЛЬСКИЙ ИНСТИТУТ ТУБЕРКУЛЕЗА И МЕЖДУНАРОДНАЯ ИНТЕГРАЦИЯ БОРЬБЫ С ТУБЕРКУЛЕЗОМ, "Вестник Центрального научно-исследовательского института туберкулеза"
Authors: Эргешов Атаджан Эргешович , Демихова Ольга Владимировна 
Published: September 2018

С целью ознакомления читателей журнала с вкладом ФГБНУ «Центральный научно-исследовательский институт туберкулеза» в международную интеграцию борьбы с туберкулезом в статье приводится исторический обзор различных аспектов международного сотрудничества ФГБНУ «Центральный научно-исследовательский институт туберкулеза», вклада его директоров и ведущих сотрудников в создание и развитие международных проектов по борьбе с туберкулезом, взаимодействия с международными организациями, зарубежными научно-исследовательскими институтами и ведущими специалистами в области борьбы с туберкулезом с момента образования Института в 1921 г. и до наших дней.
НЕКОТОРЫЕ АСПЕКТЫ ЛЕКАРСТВЕННО УСТОЙЧИВОГО ТУБЕРКУЛЕЗА В РЕСПУБЛИКЕ МОЛДОВА, "Вестник Центрального научно-исследовательского института туберкулеза"
Authors: Саин Дмитрий Онуфриевич , Александру Софья Матвеевна , Доника Анна Федоровна , Хайдарлы Ион Николаевич , Кула Евгения Николаевна , Рывняк Лидия Павловна 
Published: September 2018

Феномен лекарственной устойчивости является серьезной проблемой в контроле туберкулеза. Высокие показатели частоты штаммов резистентных МБТ регистрируются как в регионах с высокой заболеваемостью, так и с более низкой. Обращается внимание на необходимость координации мероприятий, направленных на строгое обеспечение эффективного контроля инфекции, особенно прекращения распространения ТБ МЛУ/ШЛУ/ТЛУ инфекции, которая является серьезным бременем для страны. Предварительный анализ 76 больных с широкой лекарственной устойчивостью показал необходимость проведения специального научного исследования. Исследование будет способствовать познанию, анализу и оценке масштабов рассматриваемой проблемы. Будут проанализированы основные причины развития широкой лекарственной устойчивости и контингент больных с первичной и вторичной ШЛУ/ТЛУ. Исследование должно способствовать познанию, анализу и оценке масштабов рассматриваемой проблемы, включая генетические исследования микобактерии туберкулеза.