bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological properties of metal complexes of curcumin
Authors: Shakeri Abolfazl , Panahi Yunes , Johnston Thomas P. , Sahebkar Amirhossein 
Published: April 2019

AbstractCurcumin, a naturally occurring phenolic compound isolated from Curcuma longa, has different pharmacological effects, including antiinflammatory, antimicrobial, antioxidant, and anticancer properties. However, curcumin has been found to have a limited bioavailability because of its hydrophobic nature, low‐intestinal absorption, and rapid metabolism. Therefore, there is a need for enhancing the bioavailability and its solubility in water in order to increase the pharmacological effects of this bioactive compound. One strategy is curcumin complexation with transition metals to circumvent the abovementioned problems. Curcumin can undergo chelation with various metal ions to form metallo‐complexes of curcumin, which may show greater effects as compared with curcumin alone. Promising results with metal curcumin complexes have been observed with regard to antioxidant, anticancer, and antimicrobial activity, as well as in treatment of Alzheimer's disease. The present review provides a concise summary of the characterization and biological properties of curcumin‐metal complexes. © 2019 BioFactors, 45(3):304–317, 2019
Development and validation of a simple and sensitive high‐resolution LC/MS method for determination of PF‐04620110 in dog plasma: Application to a pharmacokinetic study
Authors: He Fan , Zheng Wei , Chen Yongzhuang , Mo Chengke , Chen Yilu 
Published: April 2019

AbstractIn this study, a more sensitive and reliable quantitative method based on ultra‐high performance liquid chromatography coupled with Q‐Exactive‐Orbitrap‐MS in full‐mass scan was developed and validated for the determination of PF‐04620110 in dog plasma. After protein precipitation with acetonitrile, the sample separations were carried out on an Acquity BEH C18 column with 1 mm ammonium acetate in water and acetonitrile containing 0.1% acetic acid as mobile phase, at a flow rate of 0.4 mL/min. The assay showed excellent linearity over the concentration range of 1–2000 ng/mL with correlation coefficient >0.9980 (r > 0.9980). The LLOQ was 1 ng/mL. The inter‐ and intra‐day precision (RSD, %) was within 9.69% while the accuracy (RE, %) was in the range of −8.59–11.24%. The extraction recovery was >85.37% and the assay was free of matrix effects. PF‐04620110 was demonstrated to be stable under various processing and handing conditions. The validated method was successfully applied to the pharmacokinetic study of PF‐04620110 in dogs and the results revealed that PF‐04620110 was slowly eliminated from plasma with a clearance of 60.81 ± 7.11 mL/h/kg for intravenous administration and 81.44 ± 25.79 mL/h/kg for oral administration. The oral bioavailability was determined to be 77.89% in dogs.
lncRNA FOXD3‐AS1 is associated with clinical progression and regulates cell migration and invasion in breast cancer
Authors: Guan Yaoyao , Bhandari Adheesh , Xia Erjie , Yang Fan , Xiang Jingjing , Wang Ouchen 
Published: April 2019

For women, breast cancer is the most commonly diagnosed cancer and the leading cause of women deaths due to cancer. In recent years, increasing long noncoding RNA (lncRNA) has been discovered to be related to tumorigenesis, progression, and prognosis. FOXD3‐AS1 is a lncRNA and has been identified as a cancer‐promoting gene in glioma. By analysing the FOXD3‐AS1 expression in The Cancer Genome Atlas (TCGA) database, we found that FOXD3‐AS1 has significantly high expression in breast cancer tumour comparing with the normal tissue. And patients with low FOXD3‐AS1 expression had greater survival probability, smaller tumour size, and less distant metastasis. This leads us to peep inquisitively biological function of FOXD3‐AS1 in breast cancer. Biological assays demonstrated that silenced FOXD3‐AS1 impaired cell proliferation and inhibited cell migration and invasion in breast cancer cell lines (BT549, MDA‐MB‐231). These results suggest that FOXD3‐AS1 could play a potential diagnostics or prognostic biomarker for patients with breast cancer.Significance of the studyWe demonstrated that lncRNA FOXD3‐AS1 has significantly high expression in breast cancer cell lines comparing with the normal tissue. Besides, our findings suggested that lncRNA FOXD3‐AS1 could play a potential diagnostics or prognostic biomarker for patients with breast cancer.
Automatic Full Glycan Structural Determination through Logically Derived Sequence Tandem Mass Spectrometry
Authors: Tsai Shang‐Ting , Liew Chia Yen , Hsu Chen , Huang Shih‐Pei , Weng Wei‐Chien , Kuo Yu‐Hsiang , Ni Chi‐Kung 
Published: April 2019

AbstractGlycans have diverse functions and play vital roles in many biological systems, such as influenza, vaccines, and cancer biomarkers. However, full structural identification of glycans remains challenging. The glycan structure was conventionally determined by chemical methods or NMR spectroscopy, which require a large amount of sample and are not readily applicable for glycans extracted from biological samples. Although it has high sensitivity and is widely used for structural determination of molecules, current mass spectrometry can only reveal parts of the glycan structure. Herein, the full structures of glycans, including diastereomers, the anomericity of each monosaccharide, and the linkage position of each glycosidic bond, which can be determined by using tandem mass spectrometry guided by a logically derived sequence (LODES), are shown. This new method provides de novo oligosaccharide structural identification with high sensitivity and has been applied to automatic in situ structural determination of oligosaccharides eluted by means of HPLC. It is shown that the structure of a given trisaccharide from a trisaccharide mixture and bovine milk were determined from nearly 3000 isomers by using 6–7 logically selected collision‐induced dissociation spectra. The entire procedure for mass spectrometry measurement guided by LODES can be programmed in a computer for automatic full glycan structure identification.
Oxygen‐Vacancy‐Mediated Photocatalysis over Bi2Sn2O7: Exceptional Catalytic Activity and Selectivity
Authors: Huang Shushu , Kou Xin , He Dan , Du Chunfang , Wang Xiaojing , Su Yiguo 
Published: April 2019

AbstractA series of Bi2Sn2O7 photocatalysts was developed with the aim to regulate defect chemistry as well as electronic structure by modulating the charge states of tin species for boosting photocatalytic selective oxidation of alcohols performance. Defective centers, such as oxygen vacancies (OVs), were beneficial to enhancing the adsorption and activation of molecular oxygen. Meanwhile, the valence band edge potential of Bi2Sn2O7 with higher OVs content exhibited a distinct downshift, improving the thermodynamical driving force of photooxidation. Additionally, OVs contributed to promoting the separation efficiency of photogenerated carriers, which can be confirmed by the surface photovoltage measurement. By optimizing the electronic structure as well as defect chemistry, the optimal benzyl alcohol conversion efficiency was 76.0 % with selectivity toward benzaldehyde being about 100 %. It's found that photogenerated holes and .O2− active species played critical role in photocatalytic aerobic oxidation. This work may provide a novel insight to clarify the role of defect chemistry during the selective benzyl alcohol oxidation over Bi2Sn2O7 photocatalyst.
Simulation of Mixing in Structured Fluids with Dissipative Particle Dynamics and Validation with Experimental Data
Authors: Boccardo Gianluca , Buffo Antonio , Marchisio Daniele 
Published: April 2019

AbstractStructured fluids are simulated with dissipative particle dynamics and the predictions are validated with experimental data. The structured fluid considered is a mixture of the triblock copolymer Pluronic P103 and water. This attempt follows a first investigation with the same model parameters of a mixture with another Pluronic, L64, and water. Dissipative particle dynamics simulations are applied to identify, via a clustering algorithm, the different microstructures observed at different temperatures and compositions. This algorithm is also employed to determine the cluster mass distributions and to calculate the resulting chemical potentials associated with the different microstructures. The chemical potentials are in turn used to extract the critical micellar concentration and important shape factors. Comparison of model predictions with experimental data from the literature indicates decent agreement.
Solid‐State Photochromism by Molecular Assembly of Bis‐o‐carboranyl Siloles
Authors: Cho Yang‐Jin , Kim So‐Yoen , Lee Jie‐Won , Han Won‐Sik , Kim Chul Hoon , Son Ho‐Jin , Kang Sang Ook 
Published: April 2019

AbstractA new type of solid‐state photochromism was observed in an AB2‐type molecular assembly comprising a central silole and two peripheralo‐carborane units, and in this assembly, depending on the assembling positions of those units at the adjoining benzene ring, two different regioisomers were formed:Si‐m‐CbandSi‐p‐Cb. Each isomer showed different solid‐state photochromism depending on its solid‐state molecular conformation and was either in the crystalline or amorphous state. The crystals of eachmeta‐ orpara‐isomer, CSi‐m‐Cbor CSi‐p‐Cb, showed yellow or blue emission, and mechanically grinding those crystals into amorphous powders of ASi‐m‐Cband ASi‐p‐Cb, switched their emissions to blue and yellow, respectively. Photophysical studies revealed that the electronic interaction between silole ando‐carborane units determined the emission color. The crystal and DFT‐optimized structures each account for the crystalline and amorphous structures, respectively, and are correlated well with the electronic interactions in the molecular assembly in the solid state, thus enabling the prediction of the solid‐state molecular conformational change.
Exploring Flow Characteristics in Vanadium Redox‐Flow Batteries: Optical Measurements and CFD Simulations
Authors: Prumbohm Eva , Wehinger Gregor D. 
Published: April 2019

AbstractA thorough understanding of the flow characteristics of vanadium redox‐flow batteries is essential for their improvement. With a combined approach of CFD simulations linked with optical measurements, the distribution in a carbon felt electrode of the vanadium electrolyte as well as of an alternative fluid substitute is compared and in all three dimensions analyzed leading to qualitatively consistent results. Yet, the experimentally observed partially inhomogeneous flow distribution is not predicted by the pseudo‐homogeneous porous media CFD model.
Fein und gleichmäßig
Authors: Hechtl Cornelia 
Published: April 2019

AbstractHosokawa Alpine, einer der führenden Anbieter im Bereich Verfahrenstechnik und Folienextrusion, präsentiert auf der Powtech mit der Contraplex Weitkammermühle CW II sowie den Turboplex Feinstsichter ATP und TSP Lösungen für den 3D‐Druck.
Trocknen von APIs
Authors: Specht Martin 
Published: April 2019

AbstractAVA liefert innovative Trocknungs‐Technologie für pharmazeutische Wirkstoffe aus hochwertigen Stählen bis hin zu Hastelloy. Sie erfüllt und übertrifft die internationalen Standards GMP und FDA. Die robuste, vertikale Bauweise sowie vollautomatische Containment‐Lösungen oder manuelle Handschuhkästen gewährleisten maximale Flexibilität und industrielle Sicherheit, auch für „HPAPIs” (highly potent active pharmaceutical ingredients
Integration 4.0
Authors: Kurtz Heiko 
Published: April 2019

AbstractElektrische und pneumatische Funktionen in einer Ventilinsel zu integrieren hat sich in verfahrenstechnischen Anlagen bewährt, bspw. weil sich die kompakten elektropneumatischen Automatisierungssysteme über nur eine Busleitung ansteuern und schneller programmieren lassen. Die aktuell 4. Generation der Ventilinsel Airline SP Typ 8647 von Bürkert ist jetzt nahtlos in das neue dezentrale Peripheriesystem Simatic ET 200SP HA (High Availability) von Siemens integriert, also in einem kompakten, leistungsfähigen Automatisierungssystem zusammengefasst.
Autonom in China
Authors: Rauscher Stefan 
Published: April 2019

AbstractUm die Luftqualität in den städtischen Zentren Chinas zu verbessern, werden Lagerbestände an Eisen, Stahl und andere industrielle Produkte von traditionellen Außenstandorten in Hallenanlagen verlegt. In diesem Zuge hat die Jiangsu Binxin Special Steel Material Co. Ltd. (Binxin Steel) beschlossen, ein autonomes Schüttgutlagerverwaltungssystem von Siemens einzusetzen.
Peptide‐Modified Mo Polyoxometalate Nanoparticles Suppress Zn2+‐Induced Aβ Aggregation
Authors: Liu Yanan , Sun Jing , Gong Youcong , Zhou Hui , Chen Xu , Zhu Xufeng , Zhao Yingyu , Wen Yayu , Qin Xiuying , Liu Jie 
Published: April 2019

AbstractThe abnormal metabolism of amyloid‐β (Aβ) peptide, as one of the main components of senile plaques, plays a vital role in the pathogenesis of Alzheimer's disease (AD). Our previous work showed that polyoxometalates (POMs) that inhibited the formation of Aβ aggregates were promising for AD therapy. Here, peptide‐modified Mo polyoxometalate (Mo‐POMs) nanoparticles were synthesized by the self‐assembly of Aβ target peptide and Mo‐POMs based on our previous work. Importantly, after modifying with the Aβ target peptide, the Mo polyoxometalate (Mo‐POMs) nanoparticles exhibited enhanced blood‐brain barrier (BBB) penetration and high binding affinity with Aβ species. The interactions between the Peptide@Mo‐POMs nanoparticle and Aβ in the presence of Zn2+ were comprehensively studied using physicochemical methods (spectroscopy, ThT fluorescence, turbidity tests). Peptide@Mo‐POMs could suppress Aβ aggregation, disaggregate Aβ fibrils, and suppress Zn2+‐induced Aβ aggregation. In addition, in vitro cell experiments revealed that Peptide@Mo‐POMs could target and be well absorbed by PC12 cells, leading to the inhibition of intracellular Aβ aggregation and reduced Aβ aggregates‐induced cytotoxicity. The blood‐brain barrier (BBB) permeability of Peptide@Mo‐POMs was also revealed by the Transwell experiment. This study gives a mechanistic understanding into how Peptide@Mo‐POMs can work through a synergistic interaction with metal ions and Aβ, and provides new insights into the design and synthesis of POMs as Aβ inhibitors in the treatment of AD.
Effusion cytology of epithelioid rhabdomyosarcoma
Authors: Renshaw Andrew A. , Gould Edwin W. 
Published: April 2019

AbstractWe report a case of epithelioid rhabdomyosarcoma in a pleural effusion. In contrast to most rhabdomyosarcomas in effusions, the cells presented as cohesive clusters of atypical cells with abundant eosinophilic cytoplasm which mimicked an adenocarcinoma. Immunohistochemistry was positive for epithelial membrane antigen and muscle markers and negative for keratins.
High‐throughput single‐cell sequencing of paired TCRα and TCRβ genes for the direct expression‐cloning and functional analysis of murine T‐cell receptors
Authors: Ludwig Julia , Huber Ann‐Kathrin , Bartsch Ilka , Busse Christian E. , Wardemann Hedda 
Published: April 2019

AbstractPrecise clonal and functional assessments of the T cell receptor (TCR) repertoire diversity require paired TCRα and TCRβ gene sequence information at monoclonal level. However, available single‐cell strategies are typically limited in throughput and often do not provide full‐length DNA templates for direct gene cloning. Here, we describe a high‐throughput strategy for the unbiased amplification and automated sequence analysis of paired TCRα and TCRβ genes from primary mouse T cells. The platform links cell phenotype and TCR gene sequence information at single‐cell level. Furthermore, it enables direct functional analyses through the efficient cloning of both genes and the generation of stable TCR expressing cell lines. This highly efficient workflow is a powerful tool to determine the diversity and quality of the murine T‐cell repertoire in various settings, for example in vaccine development, infectious diseases, autoimmunity, or cancer.
1H‐decoupling and Isotopic Labeling for the Measurement of the Longitudinal Relaxation Time of Hyperpolarized 13C‐Methylenes in Choline Analogs
Authors: Uppala Sivaranjan , Gamliel Ayelet , Harris Talia , Sosna Jacob , Gomori J. Moshe , Jerschow Alexej , Katz‐Brull Rachel 
Published: April 2019

AbstractThe T1 of a hyperpolarized site in solution is a key parameter that determines the time‐window in which its NMR signals are observable. For 13C sites adjacent to protons, 1H‐decoupling has been shown to increase the hyperpolarized signal resolution and SNR. Additionally, polarization transfer to protons has shown utility in increasing the sensitivity of detection. However, 1H‐decoupling could lead to a change in the decay rate of a hyperpolarized 13C site. Here we tested this possible effect in a case where the protons are directly bound to an sp3 hyperpolarized 13C site (using [1,2‐13C2]choline) and 1H‐decoupling was applied continuously throughout the hyperpolarized decay measurement. We found that 1H‐decoupling did not lead to any significant changes in the 13C polarization decay time but did result in the expected collapse of J‐coupling and produced sharper signals. This result suggested that 1H‐decoupling did not affect the decay rate of hyperpolarized sp3 13C sites. The deuterium‐substitution approach (using [1,1,2,2‐D4,1‐13C]choline) showed a dramatic prolongation of T1. Upper bounds on the T1 of all investigated sites were calculated.
Molecular interactions between p‐coumaric acid and snake venom toxins
Authors: Cesar Pedro H. S. , Cardoso Trento Marcus Vinícius , Sales Thais A. , Marques Tamara R. , Braga Mariana A. , Ramalho Teodorico C. , Marcussi Silvana 
Published: April 2019

AbstractA large number of natural compounds, such as phenolic compounds, have been scientifically evaluated in the search for enzyme inhibitors. The interactions between the phenolic compound p‐coumaric acid and the enzymes present in snake venoms (used as research tools) were evaluated in vitro and in silico. The p‐coumaric acid was able to inhibit 31% of the phospholipase activity induced by Bothrops alternatus venom, 27% of the hemolytic activity induced by B. moojeni, 62.5% of the thrombolytic activity induced by B. jararacussu, and approximately 27% of the activity thrombosis induced by Crotalus durissus terrificus. Previous incubation of p‐coumaric acid with the venoms of B. atrox and B. jararacussu increased the coagulation time by 2.18 and 2.16‐fold, respectively. The activity of serine proteases in B. atrox and B. jararacussu venoms was reduced by 60% and 66.34%, respectively. Computational chemistry analyses suggests the specific binding of p‐coumaric acid to the active site of proteases through hydrogen and hydrophobic interactions. The phenolic compound evaluated in this work has great potential in therapeutic use to both prevent and treat hemostatic alterations, because the venom proteins inhibited by the p‐coumaric acid have high homology with human proteins that have a fundamental role in several pathologies.
Eriodictyol alleviates lipopolysaccharide‐triggered oxidative stress and synaptic dysfunctions in BV‐2 microglial cells and mouse brain
Authors: He Pandi , Yan Shikai , Wen Xin , Zhang Shuhan , Liu Zhigang , Liu Xuebo , Xiao Chunxia 
Published: April 2019

AbstractOxidative stress takes part in the development of the neurodegenerative disease. Eriodictyol, a flavonoid, commonly presents in citrus fruits, which was well‐known for its various bioactivities. The purpose of this study was to investigate the neuroprotective effects of eriodictyol on lipopolysaccharide (LPS)‐induced neuroinflammation, oxidative stress, synaptic dysfunctions, and the potential mechanisms involved. We found that eriodictyol explicitly restored LPS‐triggered the decrease of cell viability and the mitochondrial potential as well as inflammation responses via mitogen‐activated protein kinases (MAPKs) and nuclear factor κB (NF‐κB) pathways regulated by reactive oxygen species (ROS). Besides, eriodictyol alleviated LPS‐induced oxidative stress via NF‐E2‐Related factor2/Kelch‐like ECH‐associated protein 1 (Nrf2/Keap1) pathway in vivo and in vitro. Furthermore, eriodictyol reduced LPS‐elicited synaptic dysfunctions via increasing the expression of silent information regulator 1 (Sirt1). Overall, eriodictyol protects LPS‐triggered oxidative stress, neuroinflammation, and synaptic dysfunctions partially through MAPKs, NF‐κB mediated by ROS, Sirt1, and Nrf2/Keap1 signal pathways, which further supports that eriodictyol is a potentially nutritional preventive strategy for oxidative stress‐related neurodegenerative diseases.
Construction of Traf3 knockout liver cancer cell line using CRISPR/Cas9 system
Authors: Hu Wenbo , Guo Guiying , Chi Yuhua , Li Fei 
Published: April 2019

AbstractPurposeThe gene editing technology in CRISPR/Cas9 system was used to construct the Traf3 knockout HepG2 cell line to explore the role of Traf3 in the development of liver cancer.MethodsFive sgRNA sites were designed for the exons of Traf3. The recombinant plasmid of Lentiviral vector2‐Traf3‐sgRNA was constructed and transformed into Stbl3 competent cells. The recombinants were screened and sequenced, and the effectiveness of the designed gRNA was verified by sequencing. The constructed vector was transfected into HepG2 cells by lentiviral, and the monoclonal antibody was selected to detect the knockout effect of Traf3 gene in HepG2 cells by Western blot. PCR amplification and gene sequencing were performed to obtain the cell line, which the Traf3 gene was knocked out. MTT and Transwell assays were used to detect the effect of Traf3‐knockout on HepG2 cell proliferation and cell invasion, respectively.ResultsThe Lentiviral vector2‐sgRNA expression vector was successfully constructed. PCR amplification electrophoresis and gene sequencing showed that the Trep3‐knockdown HepG2 cells were successfully constructed. Compared with the wild HepG2 cells group, the proliferation and invasion ability of HepG2 cells were enhanced in the Traf3 knockout group.ConclusionKnockout Traf3 gene by CRISPR/Cas9 system enhanced the proliferation, migration, and invasion of HepG2 cells, and provided an effective tool for studying the function and mechanism of Traf3.