bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient Self‐Assembled DNA Nanoparticles through Rolling Circle Amplification for siRNA Delivery in vitro
Authors: Yao Qian , Chen Yuqi , Wu Fan , Wu Fan , Liu Chaoxing , Hong Tingting , Li Wei , Chen Yi , Zhou Xiang 
Published: April 2019

Summary of main observation and conclusionEffective and low toxicity delivery of siRNA is of great importance for clinical gene therapy. Herein, self‐assembled DNA nanoparticles (NPs) based on rolling circle amplification (RCA) with a small interfering RNA (siRNA) payload were successfully developed as a facile and efficient siRNA delivery strategy. This intracellular gene silencing strategy exhibits various advantages including low toxicity, high efficiency, and good stability. The synthesized DNA NPs serve as siRNA carriers, protecting the siRNA against nuclease degradation. We demonstrate that the obtained self‐assembled siRNA/NP/PEI system can successfully deliver enhanced green fluorescent protein (EGFP)‐siRNA into HeLa cells, realizing the same EGFP knockdown efficiency with less toxicity as that of commercial Lipofectamine 2000.
The fraction of CD117/c‐KIT‐expressing erythroid precursors predicts ESA response in low‐risk myelodysplastic syndromes
Authors: Raimbault Anna , Itzykson Raphael , Willems Lise , Rousseau Alice , Chapuis Nicolas , Mathis Stéphanie , Clauser Sylvain , Radford‐Weiss Isabelle , Bouscary Didier , Fontenay Michaela , Kosmider Olivier , Bardet Valérie 
Published: April 2019

BackgroundCompelling evidence has emerged for the relevance of flow cytometry (FC) in the diagnostic work‐up of myelodysplastic syndromes (MDS) but due to technical issues, the erythroid lineage has been under investigated, specifically in the therapeutic context.MethodsUsing the “no red cell lysis” method developed to set up the RED‐score, we specifically quantified the fraction of CD117/c‐KIT‐expressing erythroid precursors in a cohort of 144 MDS patients and studied the correlation with response to erythropoiesis‐stimulating agents (ESA) in a sub cohort of 63 low‐risk MDS patients.ResultsWe confirmed the previously reported increase in CD117/c‐KIT‐expressing erythroid precursors in a subset of MDS patients and demonstrated a strong association between a cut off of CD117/c‐KIT‐expressing erythroid precursors ≥3% and ESA response (P = 0.001), independent of red blood cell requirement. From our observations, we hypothesized that a decrease in CD117/c‐KIT‐expressing erythroid precursors could be a mechanism of ESA failure. Moreover, the fraction of CD117/c‐KIT‐expressing erythroid precursors was correlated with progression‐free survival in low‐risk MDS patients (P = 0.018). In vitro, we demonstrated in an EPO dependent cell line that CD117/c‐KIT expression is necessary for cell survival under EPO stimulation.ConclusionsThe quantification of the CD117/c‐KIT‐expressing erythroid precursors could be proposed as a new theranostic and prognostic marker in MDS treated by ESA. Future studies will be required to determine whether modulating CD117/c‐KIT expression and signaling could be used to improve anemia in MDS. © 2019 International Clinical Cytometry Society
Treat‐to‐target HbA1c and lipid profile to prolong β‐cell mass/function and optimize treatment goal attainment
Authors: Gagliardino Juan J. , Elgart Jorge , Forti Luján , Guaita María Silvina , Chantelot Jean M. 
Published: April 2019

AbstractBackgroundTo evaluate the relation between different serum lipid fractions and other known barriers to attain the HbA1c ≤ 7.0% (53 mmol/mol) target.MethodsData on 2719 patients with type 2 diabetes were collected from the five waves of the International Diabetes Mellitus Practice Study implemented in Argentina (2006 to 2012) including demographic/socioeconomic profile, clinical, metabolic (HbA1c and serum lipids) data, and treatment type and also, percentage of treatment goal attainment. Descriptive statistical analyses included ANOVA, χ2 test, and Fisher exact test and univariate and multivariate logistic regression analyses, which identified predictive factors for HbA1c ≤ 7% (53 mmol/mol).ResultsThe average age was 63 years, primary/secondary education, health insurance, 10‐year type 2 diabetes duration, most associated with cardiovascular risk factors and some microvascular/macrovascular complications; 94.5% received antihyperglycaemic drugs. Percentage of people on target: HbA1c 51.2%, blood pressure 23.5%, total cholesterol 62.6%, low‐density lipoprotein (LDL) cholesterol 38.9%, and triglycerides 61.1%. HbA1c on target depended markedly on treatment type: more of those treated with lifestyle changes and significantly fewer of those receiving insulin. Only 4.1% had all parameters simultaneously on target. Multivariate logistic regression analyses showed that achieving HbA1c ≤ 7.0% (53 mmol/mol) was associated with higher educational level, shorter diabetes duration, and having reached goals for LDL cholesterol and triglycerides, whereas opposite results were obtained with insulin treatment and longer diabetes duration.ConclusionsHigh LDL cholesterol and triglyceride levels simultaneously potentiate development/progression of chronic complications, exerting this effect in the long term by decreasing β‐cell mass/function, thereby making it more difficult to reach HbA1c values able to prevent complications.
Neuronal Plasticity in the Mushroom‐Body Calyx of Bumble Bee Workers During Early Adult Development
Authors: Kraft Nadine , Spaethe Johannes , Rössler Wolfgang , Groh Claudia 
Published: April 2019

AbstractDivision of labor among workers is a key feature of social insects and frequently characterized by an age‐related transition between tasks, which is accompanied by considerable structural changes in higher brain centers. Bumble bees (Bombus terrestris), in contrast, exhibit a size‐related rather than an age‐related task allocation, and thus workers may already start foraging at two days of age. We ask how this early behavioral maturation and distinct size variation are represented at the neuronal level and focused our analysis on the mushroom bodies (MBs), brain centers associated with sensory integration, learning and memory. To test for structural neuronal changes related to age, light exposure, and body size, whole‐mount brains of age‐marked workers were dissected for synapsin immunolabeling. MB calyx volumes, densities, and absolute numbers of olfactory and visual projection neuron (PN) boutons were determined by confocal laser scanning microscopy and three‐dimensional image analyses. Dark‐reared bumble bee workers showed an early age‐related volume increase in olfactory and visual calyx subcompartments together with a decrease in PN‐bouton density during the first three days of adult life. A 12:12 h light‐dark cycle did not affect structural organization of the MB calyces compared to dark‐reared individuals. MB calyx volumes and bouton numbers positively correlated with body size, whereas bouton density was lower in larger workers. We conclude that, in comparison to the closely related honey bees, neuronal maturation in bumble bees is completed at a much earlier stage, suggesting a strong correlation between neuronal maturation time and lifestyle in both species.
Il n'y a pas d'amour heureux pour Casanova: Chemical‐ and bio‐analysis of his Memoirs
Authors: Zilberstein Gleb , Zilberstein Roman , Zilberstein Svetlana , Fau Guillaume , D'Amato Alfonsina , Righetti Pier Giorgio 
Published: April 2019

AbstractThe original manuscript of Casanova's Memoirs is stored at the Bibliothèque Nationale de France in Paris. We have gained access to it and explored the surfaces of chapters one and two (via the ethylene vinyl acetate [EVA] film technology, i.e., of diskettes of ethylene vinyl acetate with embedded strong cation and anion exchangers and C8 resins) in search of potential diseases of the author, especially of the gonorrhea bacterium, since Casanova reported that he had several bouts of this pathology along his adventurous life. Although the bacterium was not found, we have detected high levels of HgS as red spots along the lines of the manuscript, suggesting that Casanova was using this chemical as a cure for his venereal disease. Additionally, among the several bacteria identified on the surface via mass spectrometry, we could detect traces of Streptococcus uberis, a typical animal infection, found also in humans, together with a few strains of Lactobacilli, probably present in his saliva. The EVA film technology appears to open new horizons for investigating the world Cultural Heritage.
An improved parallel compact scheme for domain‐decoupled simulation of turbulence
Authors: Fang J. , Gao F. , Moulinec C. , Emerson D.R. 
Published: April 2019

SummaryAn improved domain‐decoupled compact scheme for first and second spatial derivatives is proposed for domain‐decomposition‐based parallel computational fluid dynamics. The method improves the accuracy of previously developed decoupled schemes and preserves the accuracy and bandwidth properties of fully coupled compact schemes, even for a very large degree of parallelism, and enables the Navier‐Stokes equations to be solved independently on each processor. The scheme is analysed using Fourier analysis and error analysis, and tested on one‐dimensional wave‐packet propagation, a two‐dimensional vortex convection problem, and in the direct numerical simulation of the three‐dimensional Taylor‐Green vortex problem and turbulent channel flow. Our results demonstrate the scheme's effectiveness in performing direct numerical simulation of turbulence in terms of accuracy and scalability.
Impact of infant crying on mothers with a diagnosis of borderline personality disorder: A qualitative study
Authors: Geerling Ingrid , Roberts Rachel M. , Sved Williams Anne 
Published: April 2019

ABSTRACTMothers with borderline personality disorder (BPD) and their infants often have compromised relationships. To further understand the complexities, this study explored (a) the experiences of mothers with BPD in response to infant crying and (b) how these parenting responses impact on mothers’ interpersonal functioning with significant others. Six mothers with clinical BPD were recruited from a mother–baby psychiatric unit and interviewed using an interpretative phenomenological analysis (IPA) approach. Transcripts of the semistructured interviews were coded for themes and subthemes. Dominant themes revealed that mothers with BPD entered motherhood in a psychologically fragile state, hypersensitive to experiencing intense physiological–emotional pain and cognitive chaos in response to infant crying. Automatic maladaptive flight–fright responses including suicide attempts were common. A novel theme revealed some mothers’ split identity, and only the “mother” part could attend to infant crying. Finally, the data provided an understanding of the detrimental domino effect on close family. Mothers also highlighted gaps in accessibility of current mental health services and parenting programs, and their desire to prevent the intergenerational transmission of attachment problems and BPD symptoms to offspring. Clinical implications include antenatal BPD screening, psychoeducation about infant crying, and greater access for programs supporting the whole family to improve outcomes.
Decellularized human liver scaffold‐based three‐dimensional culture system facilitate hepatitis B virus infection
Authors: Zhang ZhenZhen , Xu HongMei , Mazza Giuseppe , Zhang MingMan , Frenguelli Luca , Liu QuanBo , Al‐Akkad Walid , Ren JiHua , Zhao RuiQiu , Ren Fang , Chen Xin , Huang AiLong , Chen Juan 
Published: April 2019

AbstractHepatitis B virus (HBV) study is hampered by lacking of idea cell model which support effective HBV infection and meanwhile recapitulate hepatocyte biology function in vivo. In this study, we developed decellularized human liver scaffolds for cell culture and further applied for HBV infection. As a result, primary human hepatocytes (PHHs) engrafted into liver scaffolds and maintained differentiation with stable albumin secretion and liver‐specific gene expression. Comparing to mono‐layer cell culture, scaffold‐based three‐dimensional (3D) culture system significantly augment HBV DNA (including cccDNA), RNA level as well as HBsAg secretion. Moreover, HepG2‐NTCP cells cultured on 3D system exhibited higher infection efficiency and longer infection period in vitro. In addition, HBV DNA level was suppressed when anti‐HBV medicine Entecavir (ETV) introduced into HepG2‐NTCP 3D system. Herein, we evaluated the potential of decellularized human liver scaffold‐based in 3D cell culture and disclosed that scaffold‐based 3D culture system can facilitate HBV infection in vitro. This 3D culture system could be further applied in HBV‐related study. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1744–1753, 2019.
Inhibition of didscoidin domain receptor 1 reduces epithelial–mesenchymal transition and induce cell‐cycle arrest and apoptosis in prostate cancer cell lines
Authors: Azizi Reza , Salemi Zahra , Fallahian Faranak , Aghaei Mahmoud 
Published: April 2019

AbstractDidscoidin domain receptor 1 (DDR1) is involved in the progression of prostate cancer metastasis through stimulation of epithelial–mesenchymal transition (EMT). So DDR1 inhibition can be a helpful target for cancer metastasis prevention. So, we studied the effects of DDR1 inhibition on EMT as well as induction of cell‐cycle arrest and apoptosis in prostate cancer cell lines. DDR1 expression was evaluated using reverse‐transcription polymerase chain reaction and western blot analysis. The EMT‐associated protein expression was determined using the western blot analysis and immunocytochemistry following treatment with various concentrations of DDR1 inhibitor. The activation of DDR1 and also downstream‐signaling molecules Pyk2 and MKK7 were determined using western blot analysis. Cell survival and proliferation after DDR1 inhibition were evaluated using 3‐(4,5‐dimethylthiazole‐2‐yl)‐2,5‐diphenyltetrazolium bromide, bromodeoxyuridine, and colony formation assays. Flow cytometry analysis was used to determine the effects of DDR1 inhibition on cell‐cycle arrest and apoptosis using annexin V/propidium iodide‐based flow cytometry. Results showed that the protein expression of N‐cadherin and vimentin were decreased whereas protein expression of E‐cadherin was increased after DDR1 inhibition. Results of our western blot analysis indicated that DDR1 inhibitor effectively downregulated P‐DDR1, P‐Pyk2, and P‐MKK7 levels. This result also showed that DDR1 inhibition decreased cell survival and proliferation, induced G1 cell‐cycle arrest, induced apoptosis by an increase in the Bax/Bcl‐2 ratio and depletion of the mitochondrial membrane potential, and also by reactive oxygen species creation in prostate cancer cells. These data show that DDR1 inhibition can result in the EMT prevention via inhibition of Pyk2 and MKK7 signaling pathway and induces cell‐cycle arrest and apoptosis in prostate cancer cell lines. Thus, this study identifies DDR1 as an important target for modulating EMT and induction of apoptosis in prostate cancer cells.
FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E‐cadherin and CD44 expression
Authors: Hong Yuling , Qin Haifeng , Li Yin , Zhang Yuhai , Zhuang Xunrong , Liu Lei , Lu Kun , Li Long , Deng Xiaoling , Liu Fan , Shi Songlin , Liu Guoyan 
Published: April 2019

AbstractCircular RNAs (circRNAs) are a new class of RNAs, and many studies have identified thousands of circRNAs in tumor cells. Fibronectin type III domain‐containing protein 3B (FNDC3B) circular RNA (circFNDC3B, circBase ID: hsa_circ_0006156) circularizes with exons 5 and 6. Gibson Assembly DNA technology was used to construct a circFNDC3B expression vector without a splice site and restriction enzyme site. We showed that circFNDC3B increased migration and invasion in gastric cancer (GC). Ectopic expression of circFNDC3B reduced the level of E‐cadherin protein to promote the epithelial–mesenchymal transition in GC. RNA immunoprecipitation assays and RNA pull‐down assays confirmed that circFNC3B increased CD44 expression, which was associated with cell adhesion, via the formation of a ternary complex of circFNDC3B‐IGF2BP3‐CD44 mRNA. These results indicated that circFNDC3B was associated with the degree of malignancy to highlight the specific characteristics of cell invasion.
Competing endogenous RNA network and prognostic nomograms for hepatocellular carcinoma patients who underwent R0 resection
Authors: Li Yuntong , Ma Bingfen , Yin Zhenyu , Liu Pingguo , Liu Jianming , Li Jie , Wang Fuqiang , Chen Huimin 
Published: April 2019

AbstractThe prognosis of hepatocellular carcinoma (HCC) after R0 resection is unsatisfactory due to the high rate of recurrence. In this study, we investigated the recurrence‐related RNAs and the underlying mechanism. The long noncoding RNA (lncRNA), microRNA (miRNA), and messenger RNA (mRNA) expression data and clinical information of 247 patients who underwent R0 resection patients with HCC were obtained from The Cancer Genome Atlas. Comparing the 1‐year recurrence group (n = 56) with the nonrecurrence group (n = 60), we detected 34 differentially expressed lncRNAs (DElncRNAs), five DEmiRNAs, and 216 DEmRNAs. Of these, three DElncRNAs, hsa‐mir‐150‐5p, and 11 DEmRNAs were selected for constructing the competing endogenous RNA (ceRNA) network. Next, two nomogram models were constructed based separately on the lncRNAs and mRNAs that were further selected by Cox and least absolute shrinkage and selection operator regression analysis. The two nomogram models that showed a high prediction accuracy for disease‐free survival with the concordance indexes at 0.725 and 0.639. Further functional enrichment analysis of DEmRNAs showed that the mRNAs in the ceRNA network and nomogram models were associated with immune pathways. Hence, we constructed a hsa‐mir‐150‐5p‐centric ceRNA network and two effective nomogram prognostic models, and the related RNAs may be useful as potential biomarkers for predicting recurrence in patients with HCC.
Male parental assistance in embryo hatching of barred‐chin blenny Rhabdoblennius nitidus
Authors: Sano Kaori , Yokoyama Risa , Kitano Takako , Takegaki Takeshi , Kitazawa Nobumasa , Kaneko Toyoji , Nishino Yoshihide , Yasumasu Shigeki , Kawaguchi Mari 
Published: April 2019

AbstractMost teleostean embryos develop and hatch without parental assistance, though some receive parental care. We focused on a paternal brood‐care species, the barred‐chin blenny (Rhabdoblennius nitidus [Günther, 1861]). As hatching approached, fanning behavior by the male parent drastically increased and then embryos hatch. In the absence of the male parent, most embryos failed to hatch. However, the hatching rate was greatly assisted by introducing an artificial water current, suggesting that paternal assistance other than for aeration is required for successful embryo hatching. Next, we analyzed genes for the hatching enzyme and egg‐envelope protein, which were successfully cloned from barred‐chin blenny, and found the expression patterns differed from those of other euteleosts. Generally, high choriolytic enzyme swells the intact egg envelope, and then low choriolytic enzyme solubilizes the swollen envelope. The expression levels of both the enzymes, but especially the latter, were much lower in barred‐chin blenny that is known in most other oviparous species. In addition, the main component of the egg envelope was changed into ChgHm and choriogenin L (ChgL) in barred‐chin blenny, whereas ChgH and ChgL for other euteleosts. These in barred‐chin blenny would result in ineffective egg‐envelope digestion because the posthatching egg envelopes were observed to be swollen but not solubilized. Male parental assistance by fanning until hatching may compensate for this insufficiency. Our study illustrates an example of the evolution of parent‐embryo interaction built on a novel relationship: Degradation of the hatching enzyme/egg‐envelope digestion system, accompanied by male parental hatching assistance.
Genetic counseling, 2030: An on‐demand service tailored to the needs of a price conscious, genetically literate, and busy world
Authors: Rashkin Misha D. , Bowes Johnathan , Dunaway Keith , Dhaliwal Jasmine , Loomis Erick , Riffle Stephen , Washington Nicole L. , Ziegler Chris , Lu James , Levin Elissa 
Published: April 2019

AbstractThe practice of genetic counseling is going to be impacted by the public's expectation that goods, services, information, and experiences happen on demand, wherever and whenever people want them. Building from trends that are currently taking shape, this article looks just over a decade into the future—to 2030—to provide a description of how the field of genetics and genetic counseling will be changed, as well as advice for genetic counselors for how to prepare. We build from the prediction that a large portion of the general public will have access to their digitized whole genome sequence anytime, any place, on any device. We focus on five topics downstream of this prediction: public health, personal autonomy, polygenic scores (PGS), evolving clinical practices, and genetic privacy.
Parenteral Nutrition Drug Shortages: A Single‐Center Experience With Rapid Process Change
Authors: Nystrom Erin M. , Bergquist Whitney J. , Wieruszewski Patrick M. , McMahon M. Molly , Barreto Erin F. 
Published: April 2019

AbstractDisruptions in the medication supply chain and consequent drug product shortages, including shortages of parenteral products used for parenteral nutrition (PN) compounding, have become an increasingly common occurrence. The amino acid solution shortage that resulted from the devastating impact of Hurricanes Maria and Irma on manufacturing facilities in Puerto Rico in 2017 necessitated a rapid, coordinated shift from use of compounded PN to commercial multichamber‐bag PN (MCB‐PN) at our hospitals. We describe our experience operationalizing this intervention via a framework that may be adapted for addressing other drug product shortages to promote rapid yet safe use of therapeutic alternatives.
Optical Nanofabrication of Concave Microlens Arrays
Authors: Liu Xue‐Qing , Yu Lei , Yang Shuang‐Ning , Chen Qi‐Dai , Wang Lei , Juodkazis Saulius , Sun Hong‐Bo 
Published: April 2019

AbstractFor the simple and versatile fabrication of nanosmooth finished microlens arrays on hard materials, an approach combining femtosecond laser modification with subsequent ion beam etching is demonstrated. This method is based on the dependence of the plasma etching rate on the laser fluence used to modify the surface. The fabricated microlenses exhibit a low surface roughness of approximately 2.5 nm, due to the high precision of the plasma etching and benefit from the smooth interface between the laser‐modified and pristine subsurface regions. Microlenses with focal lengths ranging from 60 to 100 µm are realized by controlling the laser fluence, exposure dose, and etching time. Uniform square and hexagonal microlens arrays are fabricated on both hard and ultrahard materials and glasses (fused silica, GaAs, SiC, diamond) by the same process and deliver high‐quality focusing and imaging.