bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accuracy of 24-hour ambulatory blood pressure monitoring by a novel cuffless device in clinical practice
Authors: Krisai Philipp , Vischer Annina Salome , Kilian Leo , Meienberg Andrea , Mayr Michael , Burkard Thilo 
Published: September 2018

ObjectiveRecently, a cuffless blood pressure (BP) measurement device using pulse transit time (PTT) for beat-to-beat calculation of BP values has been validated over a short time period. However, it remains unknown how values obtained with this device compare with standard ambulatory measurements over a 24-hour period. We hypothesised that BP values measured by a cuffless PTT device (TestBP) are comparable with measurements by a standard upper arm cuff-based BP device (RefBP) in clinical practice over 24 hours.MethodsBetween May and December 2017, 71 individuals were prospectively included. Cuffless using the Somnotouch-NIBP (Somnomedics GmbH, Randersacker, Germany) and cuff-based standard 24-hour BP measurements were performed simultaneously on the left and right arm, respectively. The first RefBP measurement was used as calibration measurement for the TestBP.ResultsMean (±SD) age was 49.3 (15.1) years, and 51% were male. Mean 24-hour BP for TestBP and RefBP were 140.8 (20.0) versus 134.0 (17.3) mm Hg for systolic (p<0.0001) and 85.8 (14.1)versus79.3 (11.7) mm Hg for diastolic (p<0.0001) measurements, respectively. Mean absolute systolic and diastolic disagreements between TestBP and RefBP were 10.2 (7.2) and 8.2 (5.5) mm Hg, respectively. The number (percentage) of absolute differences between the mean 24-hour BP values of the TestBP and RefBP within 5, 10 and 15 mm Hg were 23 (32.4), 43 (60.6) and 54 (74.6) for systolic and 24 (33.8), 51 (71.8) and 65 (91.6) for diastolic measurements, respectively.ConclusionIn clinical practice over 24 hours, there was a significant difference between the TestBP and RefBP with higher systolic and diastolic BP measured with the cuffless PTT device. Reasons for this difference need to be investigated.Clinical trial registrationNCT03054688; Results.
Reagent-saving immunohistochemistry for HER2 using non-contact alternating current electric field mixing
Authors: Hoshino Iku , Imai Kazuhiro , Nanjo Hiroshi , Nakamura Ryuta , Saito Yoshitaro , Fujishima Satoshi , Saito Hajime , Terata Kaori , Wakita Akiyuki , Sato Yusuke , Motoyama Satoru , Akagami Yoichi , Minamiya Yoshihiro 
Published: September 2018

AimsHuman epidermal growth factor receptor 2 (HER2)-targeted agents are an effective approach to treating patients with HER2-positive breast cancer. However, the lack of survival benefit in HER2-negative patients, as well as the toxic effects and high cost of the drugs, highlight the need for accurate and prompt assessment of HER2 status. Our aim was to evaluate the clinical utility of a novel reagent-saving immunohistochemistry method (AC-IHC) that saves HER2 antibody by taking advantage of the non-contact mixing effect in microdroplets subjected to an alternating current electric field.MethodsNinety-five specimens were used from patients diagnosed with primary breast cancers identified immunohistochemically as HER2 0/1+, 2+ or 3+ using ASCO/CAP guideline-certified standard IHC. The specimens were all tested using the conventional IHC method (1:50 antibody dilution) as well as AC-IHC (1:50 dilution) and reagent-saving AC-IHC (1:100 dilution).ResultsThe reagent-saving AC-IHC produced stable results with less non-specific staining using smaller amounts of labelled antibody. Moreover, the staining and accuracy of HER2 status evaluated with the reagent-saving AC-IHC method was equal to that achieved with standard IHC.ConclusionsThese results suggest reagent-saving AC-IHC could be used as a clinical tool for accurate and stable HER2 IHC, even when reagent concentrations vary.
Structural aspects of human lactoferrin in the iron-binding process studied by molecular dynamics and small-angle neutron scattering
Authors: Anghel Lilia , Radulescu Aurel , Erhan Raul Victor 
Published: September 2018

Abstract.Lactoferrin is a non-heme protein known for its ability to bind tightly Fe(III) ions in various physiological environments. Due to this feature lactoferrin plays an important role in the processes of iron regulation at the cellular level preventing the body from damages produced by high levels of free iron ions. The X-ray crystal structure of human lactoferrin shows that the iron-binding process leads to conformational changes within the protein structure. The present study was addressed to conformation stability of human lactoferrin in solution. Using molecular dynamics simulations, it was shown that Arg121 is the key amino acid in the stabilization of the Fe(III) ion in the N-lobe of human lactoferrin. The small-angle neutron scattering method allowed us to detect the structural differences between the open and closed conformation of human lactoferrin in solution. Our results indicate that the radius of gyration of apolactoferrin appears to be smaller than that of the hololactoferrin,$R_{g}=24.16(\pm 0.707)$Rg=24.16(±0.707)Å and$R_{g}= 26.20(\pm 1.191)$Rg=26.20(±1.191)Å, respectively. The low-resolution three-dimensional models computed for both forms of human lactoferrin in solution also show visible differences, both having a more compact conformation compared to the high-resolution structure.Graphical abstract
Comprehensive Phenotypic Characterization of Human Invasive Lobular Carcinoma Cell Lines in 2D and 3D Cultures
Authors: Tasdemir Nilgun , Bossart Emily A. , Li Zheqi , Zhu Li , Sikora Matthew J. , Levine Kevin M. , Jacobsen Britta M. , Tseng George C. , Davidson Nancy E. , Oesterreich Steffi 
Published: September 2018

AbstractInvasive lobular carcinoma (ILC) is the second most common subtype of breast cancer following invasive ductal carcinoma (IDC) and characterized by the loss of E-cadherin–mediated adherens junctions. Despite displaying unique histologic and clinical features, ILC still remains a chronically understudied disease, with limited knowledge gleaned from available laboratory research models. Here we report a comprehensive 2D and 3D phenotypic characterization of four estrogen receptor–positive human ILC cell lines: MDA-MB-134, SUM44, MDA-MB-330, and BCK4. Compared with the IDC cell lines MCF7, T47D, and MDA-MB-231, ultra-low attachment culture conditions revealed remarkable anchorage independence unique to ILC cells, a feature not evident in soft-agar gels. Three-dimensional Collagen I and Matrigel culture indicated a generally loose morphology for ILC cell lines, which exhibited differing preferences for adhesion to extracellular matrix proteins in 2D. Furthermore, ILC cells were limited in their ability to migrate and invade in wound-scratch and transwell assays, with the exception of haptotaxis to Collagen I. Transcriptional comparison of these cell lines confirmed the decreased cell proliferation and E-cadherin–mediated intercellular junctions in ILC while uncovering the induction of novel pathways related to cyclic nucleotide phosphodiesterase activity, ion channels, drug metabolism, and alternative cell adhesion molecules such as N-cadherin, some of which were differentially regulated in ILC versus IDC tumors. Altogether, these studies provide an invaluable resource for the breast cancer research community and facilitate further functional discoveries toward understanding ILC, identifying novel drug targets, and ultimately improving the outcome of patients with ILC.Significance: These findings provide the breast cancer research community with a comprehensive assessment of human invasive lobular carcinoma (ILC) cell line signaling and behavior in various culture conditions, aiding future endeavors to develop therapies and to ultimately improve survival in patients with ILC. Cancer Res; 78(21); 6209–22. ©2018 AACR.
A PET Imaging Strategy for Interrogating Target Engagement and Oncogene Status in Pancreatic Cancer
Authors: Henry Kelly E. , Dacek Megan M. , Dilling Thomas R. , Caen Jonathan D. , Fox Ian L. , Evans Michael J. , Lewis Jason S. 
Published: September 2018

Abstract

Purpose:
Pancreatic ductal adenocarcinoma (PDAC) is one of the most deadly cancers, with a 5-year survival rate of less than 10%. Physicians often rely on biopsy or CT to guide treatment decisions, but these techniques fail to reliably measure the actions of therapeutic agents in PDAC. KRAS mutations are present in &gt;90% of PDAC and are connected to many signaling pathways through its oncogenic cascade, including extracellular regulated kinase (ERK) and MYC. A key downstream event of MYC is transferrin receptor (TfR), which has been identified as a biomarker for cancer therapeutics and imaging.

Experimental Design:
In this study, we aimed to test whether zirconium-89 transferrin ([89Zr]Zr-Tf) could measure changes in MYC depending on KRAS status of PDAC, and assess target engagement of anti-MYC and anti-ERK–targeted therapies.

Results:
Mice bearing iKras*p53* tumors showed significantly higher (P &lt; 0.05) uptake of [89Zr]Zr-Tf in mice withdrawn from inducible oncogenic KRAS. A therapy study with JQ1 showed a statistically significant decrease (P &lt; 0.05) of [89Zr]Zr-Tf uptake in drug versus vehicle-treated mice bearing Capan-2 and Suit-2 xenografts. IHC analysis of resected PDAC tumors reflects the data observed via PET imaging and radiotracer biodistribution.

Conclusions:
Our study demonstrates that [89Zr]Zr-Tf is a valuable tool to noninvasively assess oncogene status and target engagement of small-molecule inhibitors downstream of oncogenic KRAS, allowing a quantitative assessment of drug delivery.
Physical Activity Level Assessed by Accelerometer and PAQ-C in Tunisian Children
Authors: Ben Jemaa Houda , Mankaï Amani , Mahjoub Faten , Kortobi Belhassen , Khlifi Sarra , Draoui Jihene , Minaoui Rim , Karmous Inchirah , Ben Hmad Halima , Ben Slama Fethi , El Hamdouchi Asma , Aguenaou Hassan , El Ati Jalila , Jamoussi Henda , Aouidet Abdallah 
Published: September 2018

<b><i>Background:</i></b> Physical activity (PA) is an important agent in the prevention of chronic diseases such as obesity. <b><i>Objectives:</i></b> To provide preliminary data on PA among school children and to correlate the PA with weight status. <b><i>Material and Methods:</i></b> A total of 40 children aged between 8 and 11 years and attending Tunisian schools were recruited. The body composition was determined by using the deuterium oxide dilution technique. PA was determined by the PAQ-C (PA Questionnaire for Older Children). An objective monitoring of PA and sedentary time was achieved by using ActiGraph GT3X + accelerometers. <b><i>Results:</i></b> PAQ-C data demonstrate that 20% of the sample had light PA levels and 80% had moderate PA levels. Times spent in sedentary (62.37%) and light (30.80%) activities were higher than that spent in moderate and vigorous (6.83%) activities. Furthermore, almost half of the sample spent more than 60 min per day in moderate-to-vigorous intensity PA (MVPA). The average MVPA was significantly higher in normal weight than overweight and obese groups (67.68 ± 20.98 vs. 49.07 ± 19.09, <i>p </i>= 0.007). The proportion of overweight children who spent more than 60 min per day in MVPA was significantly higher than that of normal-weight (60.9 vs. 29.4%; <i>p</i> = 0.049). <b><i>Conclusion:</i></b> This study indicates that half of school children comply with the health-based guidelines for PA and sedentary behavior. Our findings also suggest that obesity was associated with decrease in PA in Tunisian children.
Shame as a Culture-Specific Emotion Concept
Authors: Kollareth Dolichan , Fernandez-Dols Jose-Miguel , Russell James A. 
Published: September 2018

AbstractOn the assumption that shame is a universal emotion, cross-cultural research on shame relies on translations assumed to be equivalent in meaning. Our studies here questioned that assumption. In three studies (Ns, 108, 120, 117),shamewas compared to its translations in Spanish (vergüenza) and in Malayalam (nanakedu). American English speakers usedshamefor the emotional reaction to moral failures and its use correlated positively withguilt, whereasvergüenzaandnanakeduwere used less for moral stories and their use correlated less with the guilt words. In comparison with Spanish and Malayalam speakers’ ratings of their translations, American English speakers ratedshameandguiltto be more similar to each other.
Irenaeus’s Engagement with Rhetorical Theory in his Exegesis of the Johannine Prologue in Adversus Haereses 1.8.5-1.9.3
Authors: Harris Brendan 
Published: September 2018

Abstract
While scholars have long recognised Irenaeus’s acquaintance with rhetorical theory, the role of rhetorical theory in Irenaeus’s scriptural exegesis has only recently come to light. In the last few years, Lewis Ayres (2015) and Anthony Briggman (2015, 2016) have argued for the central importance of Greco-Roman literary and rhetorical theory to Irenaeus’s approach to scripture. This paper will build on the work of Ayres and Briggman by exploring Irenaeus’s engagement with rhetorical theory in his interpretation of the Johannine prologue in AH 1.8.5-9.3. In this paper, I will argue that Irenaeus’s exegetical strategy in AH 1.8.4-9.3 is best understood in light of the question of authorial intent as formulated in rhetorical handbooks. Specifically, I will argue that Irenaeus presents his argument as a defence of the scriptum of the prologue, and so follows the conventions laid out in rhetorical handbooks for a dispute between scriptum et voluntas.
Comparison of Agricultural Stakeholder Survey Results and Drought Monitoring Datasets during the 2016 U.S. Northern Plains Flash Drought
Authors: Otkin Jason A. , Haigh Tonya , Mucia Anthony , Anderson Martha C. , Hain Christopher 
Published: September 2018

Abstract
The evolution of a flash drought event, characterized by a period of rapid drought intensification, is assessed using standard drought monitoring datasets and on-the-ground reports obtained via a written survey of agricultural stakeholders after the flash drought occurred. The flash drought impacted agricultural production across a five-state region centered on the Black Hills of South Dakota during the summer of 2016. The survey asked producers to estimate when certain drought impacts, ranging from decreased soil moisture to plant stress and diminished water resources, first occurred on their land. The geographic distribution and timing of the survey responses were compared to the U.S. Drought Monitor and to datasets depicting anomalies in evapotranspiration, precipitation, and soil moisture. Overall, the survey responses showed that this event was a multifaceted drought that caused a variety of impacts across the region. Comparisons of the survey reports to the drought monitoring datasets revealed that the topsoil moisture dataset provided the earliest warning of drought development, but at the expense of a high false alarm rate. Anomalies in evapotranspiration were closely aligned to the survey reports of plant stress and also provided a more focused depiction of where the worst drought conditions were located. This study provides evidence that qualitative reports of drought impacts obtained via written surveys provide valuable information that can be used to assess the accuracy of high-resolution drought monitoring datasets.
Immigrants’ Use of eHealth Services in the United States, National Health Interview Survey, 2011-2015
Authors: Wang Yang , Do D. Phuong , Wilson Fernando A. 
Published: September 2018

Objectives: Little is known about the use of electronic health (eHealth) services supported by information technology in the United States among immigrants, a group that faces barriers in accessing care and, consequently, disparities in health outcomes. We examined differences in the use of eHealth services in the United States by immigration status in a nationally representative sample. Methods: We used data from the 2011-2015 National Health Interview Survey to assess use of eHealth services among US natives, naturalized citizens, and noncitizens. Our outcome variable of interest was respondent-reported use of eHealth services, defined as making medical appointments online, refilling prescriptions online, or communicating with health care professionals through email, during the past 12 months. We analyzed use of eHealth services, demographic characteristics, socioeconomic status, and health status among all 3 groups. We used multivariate logistic regression models to examine the association between immigration status and the likelihood of using eHealth services, adjusting for individual demographic, socioeconomic, and health characteristics. Results: Among 126 893 US natives, 18 763 (16.1%) reported using any eHealth services in the past 12 months, compared with 1738 of 15 102 (13.0%) naturalized citizens and 1020 of 14 340 (7.8%) noncitizens. Adjusting for socioeconomic factors reduced initial gaps: naturalized citizens (adjusted odds ratio [aOR] = 0.81; 95% confidence interval [CI], 0.75-0.87) and noncitizens (aOR = 0.81; 95% CI, 0.72-0.90) had approximately 20% lower odds of using eHealth services than did US natives. However, the differences varied by type of eHealth service. Immigrants with higher English-language proficiency were more likely to use eHealth services than were immigrants with lower English-language proficiency. Conclusions: Targeted interventions that reduce socioeconomic barriers in accessing technology and promote multilingual electronic portals could help mitigate disparities in use of eHealth services.
Efficacy of Injury Prevention Training Is Greater for High-Risk vs Low-Risk Elite Female Youth Soccer Players
Authors: De Ste Croix Mark , Hughes Jonathan , Ayala Francisco , Taylor Luke , Datson Naomi 
Published: September 2018

Background: The efficacy of robustness training for high- versus low-risk individuals within high-risk groups is currently unknown. Purpose: To explore the efficacy of robustness training on injury risk factors among female youth soccer players and to examine if high-risk athletes are greater responders to such training. Study Design: Controlled laboratory study. Methods: A total of 125 elite youth female soccer players on the English FA talent pathway were randomly selected into a training group (n = 71) or a control group (n = 54). Relative leg stiffness, 2-dimensional knee valgus and knee flexion range of motion from a single-legged countermovement jump, and probability of high knee abduction moment (pKAM) risk were all determined before and after a 16-week robustness training program. For further analysis, participants in the training group were split into groups based on risk: high risk (pKAM >0.80, n = 33) and low risk (pKAM <0.55, n = 33). Magnitude-based inferences were used to explore differences between the control and intervention groups and the high- and low-risk groups. Results: Magnitude-based inferences demonstrated significant beneficial effects in the training group for knee valgus, pKAM, and leg stiffness as compared with the control group. The control group demonstrated possible worthwhile differences in knee flexion range of motion as compared with the intervention group. The high-risk group demonstrated likely/very likely worthwhile differences versus the low-risk group for all parameters. Conclusion/Clinical Relevance: Robustness training induces significant beneficial improvements in injury risk factors among female youth soccer players. The beneficial effects of this multidimensional program are greater for those classified as high risk.
What’s “Radical” about [Feminist] Radical Political Economy?
Authors: Cohen Jennifer 
Published: September 2018

This article offers an analysis of seven articles from the Review of Radical Political Economics’ series “What ‘Radical’ Means in the 21st Century.” Without reference to feminism, the authors’ definitions of “radical” hinge critically on insight from feminist radical political economy. Instead of feminist radical political economy fitting under a broader body of political economy that coheres around radicalism, it is in feminist insight that radical political economy finds roots: according to the series’ authors, it is what makes radical political economy radical. Yet although the Union for Radical Political Economics hosted the development of the building blocks of feminist theory in economics between 1968 and 1991, feminist contributions remain largely unacknowledged. I offer strategies for repositioning feminism not as a side project but as a critical source of insight for radical political economy. JEL Classification: B54, B51, B24
The mothers in me
Authors: Katila Saija 
Published: September 2018

Abstraction of the narrative The paper aims to evoke readers’ reflective and affective capacities and thereby facilitate understanding of the multisensorial, affective, and relational nature of knowing and becoming. It highlights the role of embodied knowing in becoming by following the journey of an individual faced with sudden trauma. It describes the affective energies crossing time and space in the continuously changing sociomaterial networks of relationships encountered in different organizational settings, be they in academia, health and social services, family, or otherwise. The paper is based on an auto-ethnographic narrative of becoming a mother that connects individual experiences with cultural understandings. The narrative is an outcome of a diffractive analysis of becoming; knowing emerges during the course of a writing process in which theoretical understandings, emotions, concepts, discourses, embodied experiences, and affects come together. The paper brings out the multiplicity of contradictory discourses involved in knowing and becoming. In so doing, it highlights the entangled coexistence of body and mind, reality and imagination, public and private, reason and emotion, as well as past, present, and future.
Angiotensin IV suppresses inflammation in the brains of rats with chronic cerebral hypoperfusion
Authors: Wang Qing-Guang , Xue Xiao , Yang Yang , Gong Peng-Yu , Jiang Teng , Zhang Ying-Dong 
Published: September 2018

Introduction: This study aimed to evaluate the influence of central angiotensin IV (Ang IV) infusion on chronic cerebral hypoperfusion (CCH)-related neuropathological changes including amyloid-β (Aβ), hyperphosphorylated tau (p-tau) and the inflammatory response. Materials and methods: Rats with CCH received central infusion of Ang IV, its receptor AT4R antagonist divalinal-Ang IV or artificial cerebrospinal fluid for six weeks. During this procedure, the systolic blood pressure (SBP) was monitored, and the levels of Aβ42, p-tau and pro-inflammatory cytokines in the brain were detected. Results: Rats with CCH exhibited higher levels of Aβ42, p-tau and pro-inflammatory cytokines in the brain when compared with controls. Infusion of Ang IV significantly reduced the expression of pro-inflammatory cytokines in the brains of rats with CCH. Meanwhile, the reduction of pro-inflammatory cytokines levels caused by Ang IV was reversed by divalinal-Ang IV. During the treatment, the SBP in rats was not significantly altered. Conclusion: This study demonstrates for the first time that Ang IV dose-dependently suppresses inflammation through AT4R in the brains of rats with CCH, which is independent from SBP. These findings suggest that Ang IV/AT4R may represent a potential therapeutic target for CCH-related neurological diseases.