bluuebunny/crossref_metadata_2025_split huggingface dataset:
Muscular strength, endothelial function and cognitive disorders: state of the art
Authors: Sanchez‐Martinez Yuri , Lopez‐Lopez Jose P. , Gomez‐Montoya Isabela , Hernandez‐Quiñones Daniela , Ruiz‐Uribe Gabriela , Rincón‐Rueda Zully , Garcia Ronald G. , Lopez‐Jaramillo Patricio 
Published: November 2024

AbstractIn recent years, the ageing population has increasingly grown. This process carries a range of pathophysiological changes involving alterations in the skeletal muscle, vascular endothelium and brain function, becoming an important risk factor for developing cognitive disorders and cardiovascular diseases. With ageing, there is a decrease in muscle mass and muscle strength, and a relationship between muscle strength decrease and cognitive decline has been shown. Lower handgrip strength has been linked to memory impairment, lower global cognitive function, decreased attention and reduced visuospatial abilities in the elderly, but understanding of the underlying mechanisms that explain the link between altered skeletal muscle function and structure, endothelial dysfunction, and the role of endothelial dysfunction in the onset of cognitive disorders has been scarcely explored. This review aims to detail the cellular and molecular mechanisms by which the progressive changes associated with ageing can alter healthy skeletal muscle and endothelial function, creating an environment of oxidative stress, inflammation and mitochondrial dysfunction. These changes can lead to reduced muscle strength, and the secretion of detrimental endothelial factors, resulting in endothelial dysfunction, blood–brain barrier disruption, and damage to neurons and microglia, ultimately accelerating the onset of cognitive disorders in the elderly. In addition, we aimed to describe the mechanisms that potentially explain how preserving muscular function with resistance training could prevent brain function deterioration, including the production of different factors that allow an improved endothelial function, haemodynamic parameters and brain plasticity, ultimately delaying the onset of cognitive impairment and chronic diseases.
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Shewanella youngdeokensis sp. nov. Isolated from Marine Sediment and Sea Water of East Sea in Republic of Korea
Authors: Lee Y.-S. , Jeong H.-R. , Hwang E.-J. , Patil M. P. , Choi S. S. , Seo Y. B. , Kim G.-D. 
Published: November 2024

Abstract
The strain DAU334T, which was obtained from samples of sea water and sediments collected from the East Sea in the Republic of Korea, is a rod-shaped, Gram-stain-negative bacterium. It is facultative and positive for the oxidase and catalase enzymes. Based on the analysis of 16S rRNA sequences, it was determined that strain DAU334T had the highest degree of similarity to several strains of Shewanella species, i.e., S. schlegeliana HRKA1T (99%), S. halifaxensis HAE-EB4T (99%), S. pneumatophori SCRC-2738T (99%), S. sairae SM2-1T (99%), and S. marinintestina IK-1T (1419/1440, 99%), respectively. The complete genome sequence consisted of one contig with 4 343 646 bp, and the genome G+C content was 43.12%. The average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) between strain DAU334T and its closest relatives were 77.85‒71.10, 82.14‒67.07, and 22.60‒19.70%, respectively. The major fatty acids (>10%) of strain DAU334T were iso-C13:0 and Summed feature 3 (C16:1 w7c and/or C16:1 w6c). The respiratory quinones were ubiquinone 7 (Q7), ubiquinone 8 (Q8), menaquinone 7 (MK-7), menaquinone 8 (MK-8), methylated menaquinone 7 (MMK-7), and an unidentified one. According to the results of polyphasic analyses, the strain DAU334T belongs to a novel species within the genus Shewanella, for which the name Shewanellayoungdeokensis sp. nov. (type strain DAU334T = KCTC 72428T = JCM 35745T = KCCM 43479T) is proposed.
Genome Analysis and Reconstruction of Metabolic Pathways of Amino Acid and Betaine Degradation in the Haloalkaliphilic Bacterium Anoxynatronum sibiricum
Authors: Detkova E. N. , Boltyanskaya Yu. V. , Pimenov N. V. , Mardanov A. V. , Kevbrin V. V. 
Published: November 2024

Abstract
The genome of the haloalkaliphilic anaerobe Anoxynatronum sibiricum Z-7981T, isolated previously from the soda Lake Nizhnee Beloe (Republic of Buryatia, Russia), was analyzed. The microorganism was shown to be capable of utilizing betaine as an electron acceptor in the Stickland reaction. Supplementing the medium with betaine had a stimulating effect on the growth of A. sibiricum on those amino acids that could be utilized individually. Furthermore, it enabled the strain to utilize several additional amino acids that could not support its growth in the absence of the acceptor. Based on the genomic characteristics and experimental growth data, metabolic schemes are proposed to describe amino acid degradation in the presence and absence of betaine. The schemes for threonine, glutamate, and lysine utilization together with betaine have been compiled for the first time. For all amino acids used, the qualitative and quantitative composition of metabolic products was determined and the stoichiometric substrate/product ratios were obtained. Balance equations were derived for all identified variants of the Stickland reaction, including those not previously described in the literature.
Methylomarinum roseum sp. nov., a Novel Halophilic Methanotrophic Bacterium from the Hypersaline Lake Elton
Authors: Suleimanov R. Z. , Oshkin I. Y. , Danilova O. V. , Suzina N. E. , Dedysh S. N. 
Published: November 2024

Abstract
The genus Methylomarinum accommodates aerobic motile non-pigmented methanotrophic bacteria, which were isolated from marine habitats and require NaCl for growth. Until recently, this genus contained a single species, M. vadi. Here, we describe a novel Methylomarinum representative, strain Ch1-1T, obtained from the sediments of the River Chernavka at its confluence with the hypersaline Lake Elton, Russia. Strain Ch1-1T was represented by gram-negative, pink-pigmented, motile rods or ovoids, which multiplied by binary fission and possessed the particulate methane monooxygenase (pMMO). These bacteria grew on methane within the temperature range of 5–42°C (optimum at 30–35°C) and at рH 6.3–7.5 (optimum at 6.5–6.8). Growth on methanol was inconsistent. NaCl was required for growth, which was observed in liquid media only within a NaCl concentration range of 0.5–10% (w/v). Best growth was recorded at a NaCl concentration of 3.5–4.0% (w/v). The 16S rRNA gene sequence of strain Ch1-1T displayed 97.09–97.24% similarity to the corresponding gene fragments of described representatives of M. vadi. The genome of strain Ch1-1T was 4.8 Mb in size and contained 3 rRNA operons and about 4400 protein-coding genes, including the gene cluster pmoCAB coding for pMMO, a complete set of genes for the function of the ribulose monophosphate pathway as well as genes necessary for ectoine and sucrose biosynthesis. The DNA G+C content was 50.7 mol %. The average nucleotide identity determined for genomes of strain Ch1-1T and M. vadi IT-4T was 78.8%. Based on the number of genotypic and phenotypic differences, we propose to classify this isolate as representing a novel species of the genus Methylomarinum, M. roseum sp. nov. strain Ch1-1T (=VKM B-3852T = UQM 41855T) is the type strain of the newly proposed species.
Strongly One-Sided ( b,c ) -Inverses in Rings
Authors: Wu Cang , Chen Jianlong 
Published: November 2024

We give some new characterizations of strongly one-sided [Formula: see text]-invertibility of elements in rings, and establish the relations between one-sided [Formula: see text]-inverses and strongly one-sided [Formula: see text]-inverses. When [Formula: see text], Kaplansky's theorem on the number of one-sided inverses is generalized to the case of strongly one-sided [Formula: see text]-inverses. Moreover, we give some new characterizations of Dedekind-finite rings from the viewpoint of [Formula: see text]-finiteness, and thus answer a question in [L. Wang, D. Mosić, The one-sided inverse along two elements in rings, Linear Multilinear Algebra 69 (13) (2021) 2410–2422].
Development Process of a Holistic Assessment Questionnaire to Measure and Monitor Cancer‐Related Fatigue
Authors: Wijlens Kim A. E. , Witteveen Annemieke , Beenhakker Lian , Siesling Sabine , Vollenbroek-Hutten Miriam M. R. , Bode Christina 
Published: November 2024

Purpose: To understand the consequences of diseases and treatment such as cancer and thus the needs of patients for surveillance and care and to improve quality of life, patients should be assessed using a holistic approach. However, instruments to create such a holistic view do not exist and the development presents unique challenges. Therefore, this study presents a method for the development of a holistic assessment questionnaire using cancer‐related fatigue (CRF) as a case.Method: We started with (1) the definition of our construct of interest (CRF) on the theme level followed by (2) item selection, an iterative process of searching for validated questionnaires that together cover the full holistic construct. The construct definition on theme level (1) was too broad and was, therefore, redefined on the element level (construct > theme > element) based on interviews with relevant stakeholders. Hereafter, item selection (2) was performed on the element level based on a priority list, psychometric properties (e.g., discriminative parameter value) and consultation of experts and future users. Lastly, (3) items were reformulated.Results: Initial CRF construct definition (1) resulted in 110 relevant validated questionnaires with over three thousand items, requiring a construct redefinition on element level. Seventy‐two items from 21 validated questionnaires were included (2) in the preliminary holistic assessment questionnaire. For item reformulation (3), easy language was used to better suit the target population.Conclusion: Tailoring care to the individual requires a holistic view. This article presents a novel method to develop a holistic assessment questionnaire, including an example for CRF, with several recommendations for cancer‐specific instrument development. Although the development process of a holistic assessment questionnaire is time‐consuming, more late and long‐term effects of cancer are multidimensional and could benefit from a holistic approach in their assessment to enable personalised care, thereby improving quality of life and reducing societal impact.
Enhancing Medication History Accuracy in a Tertiary Hospital: Pharmacist‐Led Counseling With Nationwide Medication History Sharing Program Versus Conventional Methods
Authors: Chae Hyunwoo , Yoon Subin , Park Soyoung , Jeong Kim A , Heo Kyu-Nam , Kim Yookyung , Seok Jeong Yeon , Kim Sung Hwan , Yun Taeyoung , Park Ji Hyeon , Cho Yoon Sook , Kim Young Tae , Lee Hyun Joo , Lee Ju-Yeun 
Published: November 2024

Aims: Medication discrepancies during transitions of care pose substantial risks, particularly in older patients receiving complex medication regimens. Obtaining the best possible medication history (BPMH) is crucial to minimize this risk. This study aimed to evaluate the effectiveness of a pharmacist‐led BPMH using various information sources including a nationwide medication history sharing program compared to conventional interviews in a tertiary hospital setting. In addition, we identified factors contributing to medication inaccuracies.Methods: A single‐center prospective study involving older patients scheduled for thoracic surgery at a tertiary hospital was conducted. Medication histories obtained through conventional interviews during the initial assessment on admission were compared with those obtained through BPMH by pharmacist‐led interviews. Logistic regression analysis was used to identify factors associated with medication inaccuracies.Results: This study included 216 patients, a significant proportion of whom were experiencing polypharmacy. The pharmacist‐led BPMH approach demonstrated superior accuracy in documenting medication histories, with conventional methods showing inaccuracies in over 70% of the cases. Factors that significantly increased the likelihood of inaccuracies included the number of medications and number of prescribers. Nonoral and as‐needed medications are particularly prone to inaccuracies.Conclusions: Pharmacist‐led BPMH significantly improved medication history accuracy compared to conventional methods, which had inaccuracies in over 70%. It is important to be careful when obtaining BPMH for older adults, particularly those with complex medication regimens, nonoral medications, or PRN medications, to enhance patient safety and reduce medication‐related risks.Trial Registration: Clinical Trial Registry identifier: KCT-006813.
Factors Associated With Nonattendance for Osteoporosis Evaluation Following Fragility Fracture
Authors: Seyok Thany , Collins Jamie E. , Hodys Cole , Erikson Samantha J. , Perez Menendez Samantha , Earp Brandon E. , Charles Julia F. 
Published: November 2024

Introduction: This study assessed patient demographic factors associated with nonattendance for osteoporosis evaluation after being referred to our Bone Health Clinic (BHC), a hospital‐based outpatient Fracture Liaison Service (FLS), for a fragility fracture.Methods: 507 patients sustaining a fragility fracture were referred to the BHC over a 39‐month period. Retrospective chart review was conducted to capture osteoporosis evaluation attendance rates and demographic factors (age, gender, race, area deprivation index, insurance type, and fracture type). A post‐fracture follow‐up visit with either the BHC or another provider in which osteoporosis was noted in the assessment was considered attendance for osteoporosis evaluation. Nonattendance was determined at a cutoff of one year after the fracture date.Results: Of the 507 patients referred to the BHC following a fragility fracture, 177 patients attended osteoporosis evaluation with either the BHC or a primary care provider. Nonattendance was associated with older age (p = 0.0075), having private health insurance (p = 0.0434), and recent hip fracture (p < 0.0001). Attendance was associated with having government health insurance (p = 0.0103).Conclusion: Inpatient evaluation and treatment for osteoporosis should be considered in patients who are older or have sustained a hip fracture as they may have more difficulty attending post‐fracture appointments.
PALS1-dependent modulations of mRNA profiles in MDCK II cells grown in non-confluent monolayers and three-dimensional cysts
Authors: Schughart Klaus , Möller-Kerutt Annika , Höffken Verena , Nedvetsky Pavel , Groh Ann-Christin , Braun Daniela Anne , Pavenstädt Hermann , Weide Thomas 
Published: November 2024

AbstractIn epithelia, apicobasal cell polarization is closely linked to cell-cell contact formation, both controlled by the conserved Crumbs (CRB) complex, which includes the transmembrane protein Crumbs (CRB3a) and adapter proteins PALS1, PATJ, and LIN7c. In MDCK II cells, a model for cell polarization, depletion of PALS1 - which binds to all CRB components - leads to defective cell polarization and improper distribution of tight junction proteins, resulting in severe epithelial barrier defects in 3D cyst models. This study investigated whether this phenotype is associated with transcriptional changes by analyzing wildtype (WT) and PALS1 knockout (KO) MDCK II cell lines grown under non-confluent conditions and in 3D cyst cultures. Our results indicate that the transition from non-confluent cells to 3D cysts involves numerous differentially expressed genes (DEGs) in both WT and KO cells. Importantly, the analyses revealed significant overlaps between WT and KO cells in their maturation processes, suggesting that most identified DEGs are linked to differentiation from non-confluent to polarized MDCK cells and likely not a result of PALS1 deficiency. Gene Ontology (GO) enrichment and over-representation analyses using REACTOME and KEGG databases confirmed these similarities. In contrast, the direct comparison of WT and KO cells at the two stages showed fewer DEGs and overlaps in associated biological processes and signaling pathways. DEGs associated with the 3D stage, in which the phenotype manifests, contain DEGs and pathways that were predominantly linked to cell cycle linked processes, centromere assembly, or DNA replication. Furthermore, the transcription of genes encoding key junction proteins, additional polarity proteins, and cell-substrate interaction proteins is less affected by the loss of PALS1, indicating that PALS1 influences the transcriptional profiles in epithelial cells as a modulating factor.
A look back at the strike by Mozambican doctors in 2013: what can we learn?
Authors: Manguele Alexandre Lourenço Jaime , Craveiro Isabel , Sidat Mohsin , Sengo Dulnério Barbosa , Cabral António Jorge Rodrigues , Ferrinho Paulo 
Published: November 2024

Abstract
Background
The occurrence of strikes in the health sector has been an increasing concern around the world, given their negative impact on the provision of services and care to patients. The Mozambican doctors' strike in 2013 2013 is considered by many to be the largest of a kind in the country's history, and marked the changes which are still a matter of debate. The aim of this study is to understand the causes, strategies and perceived impact of this strike from the perspective of the main actors involved, taking a look back at everything that happened, including the backstage and tense moments during the negotiations. These details have been little covered in similar studies and are important for a better understanding and management of this type of movement.

Methods
This is a qualitative study with a phenomelogical approach that consisted of semi-structured interviews with the main players involved in the strike movement, and analysis of documents produced around this movement. Non-probabilistic snowball sampling was used to select participants until data saturation was reached. The interviews were transcribed and imported into Nvivo version 12, and the data was analysed using content analysis to identify themes related to the research questions.

Results
The doctors were demanding better salaries, career prospects and working conditions. Failure to fulfil agreements, threats from the government and a lack of communication are believed to have precipitated the strike. Faced with staff shortages, the government restricted services, prioritised urgent cases, and patients saw services slowed down, their care delayed, a lack of medicines in health units and a loss of confidence in the healthcare system. Although the strike contributed to the approval of the Doctors' Statute, it led to the interruption of postgraduate studies, transfers and suspensions of professionals.

Conclusion
The strike was motivated by aspects associated with salaries and working conditions. Some of the approaches adopted further distanced the parties and delayed consensus. The strike had negative consequences for everyone, especially patients. This study provides important lessons for improving strike prevention and management strategies in the health sector.
Influence of trust on the acceptance of the RTS,S malaria vaccine in the Kassena-Nankana districts of Ghana
Authors: Bugase Eustace , Tindana Paulina 
Published: November 2024

Abstract
Background
Vaccines have increasingly become some of the most effective public health tools for promoting health and reducing the burden of infectious diseases. The availability of a malaria vaccine for routine use will be a major milestone, nonetheless, trust by the public for the vaccine could pose a major challenge for its acceptance. Documented evidence such as the boycott of the oral polio vaccine in northern Nigeria and the failure of the Ebola vaccine trial in Ghana among others highlight the impact of public trust on vaccine acceptance.

Methods
This is an explorative cross-sectional mixed-method study conducted in the Kassena Nankana districts. The study was conducted in the Kassena Nankana Districts between May and December 2020. A total of 390 structured questionnaires were administrated to mothers and caregivers of children under five years of age while 15 in-depth interviews were conducted with mothers and health workers. STATA software Version 16.0 was used to interpret the quantitative data, where bivariate and multivariate regression analysis was performed to determine the influence of trust on vaccine acceptance while QSR NVivo 12 software was used to code the qualitative data to aid the thematic analysis.

Results
The results revealed that the level of knowledge of the RTS,S vaccine among participants was high. About 95.4% of the mothers had good knowledge of the malaria vaccine and more than half 61.2% of them got information about the vaccine from the health facility. The level of trust for the malaria vaccine was equally high with 91.4% of the mothers reporting that the vaccine treats childhood malaria. In a bivariate analysis, educational status (P = 0.013), ethnicity (P = 0.008), marital status (P = 0.041), education on the vaccine and perceived ineffectiveness P < 0.05, and trust for the malaria vaccine (P < 0.05) were found to be statistically associated with vaccine acceptance. Compared with participants who agree that vaccines are harmless to children, those who disagree were significantly less likely to accept vaccines (OR = 0.25, 95%CI [0.08, 0.83], p = 0.017). The qualitative data correspondingly revealed that mothers trusted vaccines which thus accounted for the high uptake of the malaria vaccine in the districts.

Conclusion
The results of this study suggest that trust in the malaria vaccine is critical for its uptake. Therefore, efforts towards improving acceptance of the vaccine should be focused on building and sustaining trust for the vaccine among mothers and community members.
Analytic performance of ScreenFire HPV RS assay Zebra BioDome format and its potential for large-scale population HPV screening
Authors: Wang Jun , Imade Godwin , Akanmu Alani S. , Musa Jonah , Anorlu Rose , Zheng Yinan , Garcia-Bedoya Olga , Sanchez Gloria I. , Belinson Jerome , Kim Kyeezu , Maiga Mamoudou , Gursel Demirkan B. , Sagay Atiene S. , Ogunsola Folasade T. , Murphy Robert L. , Hou Lifang 
Published: November 2024

Abstract
Background
Easy-to-use, rapid, scalable, high-throughput, and cost-effective HPV tests are urgently needed for low-resource settings. Atila Biosystems’ high-throughput, cost-effective, and clinically validated ScreenFire HPV Risk Stratification (RS) assay identifies 13 high risk HPV (hrHPV) in 4 groups based on their oncogenic risk (i.e., HPV16, HPV18/45, HPV31/33/35/52/58, and HPV51/59/39/56/68). The current standard format is subject to laboratory contamination, which is common for any molecular PCR test. To overcome this drawback, Atila has recently upgraded it into an innovative, contamination-free Zebra BioDome format. The contamination-free feature makes this novel assay format more suitable for large-scale community- and population-based cervical screening. This study evaluated the analytical performance of the Zebra BioDome format.

Methods
We conducted a study to test the analytical performance of Zebra Biodome format in comparison to the results of using the ScreenFire HPV RS assay standard format on Biorad CFX-96 real-time PCR instrument. We used overall agreement rate and unweighted kappa value to compare the performance.

Results
The overall agreement for detection of hrHPV was 96.0% with unweighted kappa value 0.94 (95% confidence interval: 0.90–0.98). The agreement rates between hrHPV genotype 16 and risk stratification genotype group (HPV18/45, HPV31/33/35/52/58, and HPV51/59/39/56/68) were all > 97.5%.

Conclusion
The innovative ScreenFire HPV RS assay Zebra BioDome format produced highly concordant results with the standard format. The shared features by the two assay formats, such as easy-to-use, high throughput, cost-appropriate, and no requirements for DNA extraction. The unique contamination-prevention feature along with no requirement of preparation of reagents make the Zebra BioDome format more suitable for large-scale HPV screening to reduce global cervical cancer burden.