bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meteorological and Hydrological Drought on the Loess Plateau, China: Evolutionary Characteristics, Impact, and Propagation
Authors: Wu Jingwen , Miao Chiyuan , Zheng Haiyan , Duan Qingyun , Lei Xiaohui , Li Hu 
Published: October 2018

AbstractDrought is the most recurrent and destructive hazard in arid and semiarid regions and will only become more complex under climate change. It is vital to characterize the various types of drought, to investigate the potential factors affecting different types of drought, and to assess the relationship between drought types. In this study, the Standardized Precipitation Index and the Standardized Runoff Index were used to characterize meteorological and hydrological drought, respectively, to investigate drought characteristics and mechanisms in 17 catchments on the Loess Plateau from 1961 to 2013. Furthermore, the propagation time from meteorological to hydrological drought was explored and the potential factors influencing drought propagation time were investigated. The results indicate that the Loess Plateau has experienced an increased tendency toward both meteorological and hydrological droughts over the period 1961–2013, with hydrological drought more serious than meteorological drought at various drought assessment time scales. Moreover, average drought duration and severity were greater for hydrological drought than meteorological drought. Maximum 5‐day precipitation (Rx5day) was the dominant extreme climate index for explaining variance in meteorological drought at the annual time scale. Owing to the greater complexity underlying hydrological drought, Rx5day, the number of warm days (Tx90p), and the number of warm nights (Tn90p) all contribute to the variance in hydrological drought. Furthermore, the percentage of forested land had a significant positive association (p < 0.001) with propagation time, whereas the percentage of land given over to pasture had a significant negative association (p < 0.001) with propagation time.
Groundwater Controls on Postfire Permafrost Thaw: Water and Energy Balance Effects
Authors: Zipper Samuel C. , Lamontagne‐Hallé Pierrick , McKenzie Jeffrey M. , Rocha Adrian V. 
Published: October 2018

AbstractFire frequency and severity are increasing in high‐latitude regions, but the degree to which groundwater flow impacts the response of permafrost to fire remains poorly understood. Here we use the Anaktuvuk River Fire (Alaska, USA) as an example for simulating groundwater‐permafrost interactions following fire. We identify key thermal and hydrologic parameters controlling permafrost response to fire both with and without groundwater flow, and separate the relative influence of changes to the water and energy balances on active layer thickness. Our results show that mineral soil porosity, which influences the bulk subsurface thermal conductivity, is a key parameter controlling active layer response to fire in both the absence and presence of groundwater flow. However, including groundwater flow in models increases the perceived importance of subsurface hydrologic properties, such as the soil permeability, and decreases the perceived importance of subsurface thermal properties, such as the thermal conductivity of soil solids. Furthermore, we demonstrate that changes to the energy balance (increased soil temperature) drive increased active layer thickness following fire, while changes to the water balance (decreased groundwater recharge) lead to reduced landscape‐scale variability in active layer thickness and groundwater discharge to surface water features such as streams. These results indicate that explicit consideration of groundwater flow is critical to understanding how permafrost environments respond to fire.
High-throughput multiplexed fluorescence-activated droplet sorting
Authors: Caen Ouriel , Schütz Simon , Jammalamadaka M. S. Suryateja , Vrignon Jérémy , Nizard Philippe , Schneider Tobias M. , Baret Jean-Christophe , Taly Valérie 
Published: October 2018

AbstractFluorescence-activated droplet sorting (FADS) is one of the most important features provided by droplet-based microfluidics. However, to date, it does not allow to compete with the high-throughput multiplexed sorting capabilities offered by flow cytometery. Here, we demonstrate the use of a dielectrophoretic-based FADS, allowing to sort up to five different droplet populations simultaneously. Our system provides means to select droplets of different phenotypes in a single experimental run to separate initially heterogeneous populations. Our experimental results are rationalized with the help of a numerical model of the actuation of droplets in electric fields providing guidelines for the prediction of sorting designs for upscaled or downscaled microsystems.
Prefrontal expectancy and reinforcement-driven antidepressant placebo effects
Authors: Peciña M. , Heffernan J. , Wilson J. , Zubieta J. K. , Dombrovski A. Y. 
Published: October 2018

AbstractPlacebo responses in depression exemplify how expectancies and appraisals impact mood. Cognitive and neural mechanisms underlying these responses are still poorly understood, partly due to the difficulty of simulating antidepressant effects and manipulating mood experimentally. To address these challenges, we developed an acute antidepressant placebo experiment involving the intravenous administration of a “fast-acting antidepressant” and a trial-by-trial sham fMRI “neurofeedback” manipulation, purporting to reveal mood-relevant neural responses. Twenty volunteers with major depression underwent this experiment while rating their expected and actual mood improvement. Mixed-effects analyses of trial-by-trial ratings revealed that the “drug” infusion cues induced higher expectancies of mood improvement, while both the “drug” infusion cue and the sham neurofeedback induced a reported mood improvement. Neurofeedback of greater magnitude, compared to lower magnitude, recruited the lateral prefrontal cortex (lPFC). Individuals with greater lPFC responses to neurofeedback displayed: (1) greater effect of previous mood improvement on expectancy ratings and (2) greater effect of sham neurofeedback on mood improvement. Behavioral antidepressant placebo effects were additionally moderated by changes in peripheral β-endorphin plasma levels and depressive symptomatology. These data demonstrate the feasibility of trial-by-trial manipulation of antidepressant placebo-associated expectancies and their reinforcement. We provide initial insights into the role of the lPFC in the interplay between placebo-induced expectancies and mood, as well as preliminary evidence for the role of the opioid system in antidepressant placebo effects.
Positive feedback loop between mitochondrial fission and Notch signaling promotes survivin-mediated survival of TNBC cells
Authors: Chen Li , Zhang Jing , Lyu Zhuomin , Chen Yibing , Ji Xiaoying , Cao Haiyan , Jin Mingpeng , Zhu Jianjun , Yang Jin , Ling Rui , Xing Jinliang , Ren Tingting , Lyu Yonggang 
Published: October 2018

AbstractMitochondrial morphology is remodeled by continuous dynamic cycles of fission and fusion. Emerging data have shown that the disturbance of balance between mitochondrial fission and fusion is involved in the progression of several types of neoplasms. However, the status of mitochondrial dynamics and its potential biological roles in breast cancer (BC), particularly in triple negative BC (TNBC) are not fully clear. Here, we reported that the mitochondrial fission was significantly increased in BC tissues, especially in the TNBC tissues, when compared with that in the corresponding peritumor tissues. Meanwhile, our data showed that Drp1 was upregulated, while Mfn1 was downregulated in TNBC. Moreover, elevated mitochondrial fission was associated with poorer prognosis in TNBC patients. Mitochondrial fission promoted the survival of TNBC cells both in vitro and in vivo. Furthermore, we identified a positive feedback loop between mitochondrial fission and Notch signaling pathway in TNBC cells, as proved by the experimental evidence that the activation of Notch signaling enhanced Drp1-mediated mitochondrial fission and Drp1-mediated mitochondrial fission in turn promoted the activation of Notch signaling, which ultimately promoted the cell survival of TNBC via increasing survivin expression level. Inhibition of either Notch1 or Drp1 significantly impaired the activation of the other, leading to the suppression of TNBC cell survival and proliferation. Collectively, our data reveal a novel mechanism that the positive feedback loop between mitochondrial fission and Notch signaling promotes the survival, proliferation and apoptotic resistance of TNBC cells via increasing survivin expression and thus favors cancer progression.
Complex assembly from planar and twisted π-conjugated molecules towards alloy helices and core-shell structures
Authors: Lei Yilong , Sun Yanqiu , Zhang Yi , Zhang Hongyang , Zhang Haihua , Meng Zhengong , Wong Wai-Yeung , Yao Jiannian , Fu Hongbing 
Published: October 2018

AbstractIntegrating together two dissimilar π-conjugated molecules into controlled complex topological configurations remains a largely unsolved problem owing to the diversity of organic species and their respective different assembly features. Here, we find that two structurally similar organic semiconductors, 9,10-bis(phenylethynyl)anthracene (BA) and 5,12-bis(phenylethynyl)naphthacene (BN), co-assemble into two-component helices by control of the growth kinetics as well as the molar ratio of BA/BN. The helical superstructures made of planar and twisted bis(phenylethynyl) derivatives can be regarded as (BA)x(BN)1−x alloys, which are formed due to compatible structural relationship between BA and BN. Moreover, epitaxial growth of (BA)x(BN)1−x alloy layer on the surface of BA tube to form BA@(BA)x(BN)1−x core-shell structure is also achieved via a solute exchange process. The precise control over composition and morphology towards organic alloy helices and core-shell microstructures opens a door for understanding the complex co-assembly features of two or more different material partners with similar structures.
Incompatibility of the circadian protein BMAL1 and HNF4α in hepatocellular carcinoma
Authors: Fekry Baharan , Ribas-Latre Aleix , Baumgartner Corrine , Deans Jonathan R. , Kwok Christopher , Patel Pooja , Fu Loning , Berdeaux Rebecca , Sun Kai , Kolonin Mikhail G. , Wang Sidney H. , Yoo Seung-Hee , Sladek Frances M. , Eckel-Mahan Kristin 
Published: October 2018

AbstractHepatocyte nuclear factor 4 alpha (HNF4α) is a master regulator of liver-specific gene expression with potent tumor suppressor activity, yet many liver tumors express HNF4α. This study reveals that P1-HNF4α, the predominant isoform expressed in the adult liver, inhibits expression of tumor promoting genes in a circadian manner. In contrast, an additional isoform of HNF4α, driven by an alternative promoter (P2-HNF4α), is induced in HNF4α-positive human hepatocellular carcinoma (HCC). P2-HNF4α represses the circadian clock gene ARNTL (BMAL1), which is robustly expressed in healthy hepatocytes, and causes nuclear to cytoplasmic re-localization of P1-HNF4α. We reveal mechanisms underlying the incompatibility of BMAL1 and P2-HNF4α in HCC, and demonstrate that forced expression of BMAL1 in HNF4α-positive HCC prevents the growth of tumors in vivo. These data suggest that manipulation of the circadian clock in HNF4α-positive HCC could be a tractable strategy to inhibit tumor growth and progression in the liver.
Nanodiamond autophagy inhibitor allosterically improves the arsenical-based therapy of solid tumors
Authors: Cui Zhifen , Zhang Yu , Xia Kai , Yan Qinglong , Kong Huating , Zhang Jichao , Zuo Xiaolei , Shi Jiye , Wang Lihua , Zhu Ying , Fan Chunhai 
Published: October 2018

AbstractArsenic trioxide (ATO) is a successful chemotherapeutic drug for blood cancers via selective induction of apoptosis; however its efficacy in solid tumors is limited. Here we repurpose nanodiamonds (NDs) as a safe and potent autophagic inhibitor to allosterically improve the therapeutic efficacy of ATO-based treatment in solid tumors. We find that NDs and ATO are physically separate and functionally target different cellular pathways (autophagy vs. apoptosis); whereas their metabolic coupling in human liver carcinoma cells remarkably enhances programmed cell death. Combination therapy in liver tumor mice model results in ~91% carcinoma decrease as compared with ~28% without NDs. Treated mice show 100% survival rate in 150 days with greatly reduced advanced liver carcinoma-associated symptoms, and ~80% of post-therapy mice survive for over 20 weeks. Our work presents a novel strategy to harness the power of nanoparticles to broaden the scope of ATO-based therapy and more generally to fight solid tumors.
Dimethyl fumarate is an allosteric covalent inhibitor of the p90 ribosomal S6 kinases
Authors: Andersen Jacob Lauwring , Gesser Borbala , Funder Erik Daa , Nielsen Christine Juul Fælled , Gotfred-Rasmussen Helle , Rasmussen Mads Kirchheiner , Toth Rachel , Gothelf Kurt Vesterager , Arthur J. Simon C. , Iversen Lars , Nissen Poul 
Published: October 2018

AbstractDimethyl fumarate (DMF) has been applied for decades in the treatment of psoriasis and now also multiple sclerosis. However, the mechanism of action has remained obscure and involves high dose over long time of this small, reactive compound implicating many potential targets. Based on a 1.9 Å resolution crystal structure of the C-terminal kinase domain of the mouse p90 Ribosomal S6 Kinase 2 (RSK2) inhibited by DMF we describe a central binding site in RSKs and the closely related Mitogen and Stress-activated Kinases (MSKs). DMF reacts covalently as a Michael acceptor to a conserved cysteine residue in the αF-helix of RSK/MSKs. Binding of DMF prevents the activation loop of the kinase from engaging substrate, and stabilizes an auto-inhibitory αL-helix, thus pointing to an effective, allosteric mechanism of kinase inhibition. The biochemical and cell biological characteristics of DMF inhibition of RSK/MSKs are consistent with the clinical protocols of DMF treatment.
Engineering the breaking of time-reversal symmetry in gate-tunable hybrid ferromagnet/topological insulator heterostructures
Authors: Lee Joon Sue , Richardella Anthony , Fraleigh Robert D. , Liu Chao-xing , Zhao Weiwei , Samarth Nitin 
Published: October 2018

AbstractStudying the influence of broken time-reversal symmetry on topological materials is an important fundamental problem of current interest in condensed matter physics and its understanding could also provide a route toward proof-of-concept spintronic devices that exploit spin-textured topological states. Here we develop a new model quantum material for studying the effect of breaking time-reversal symmetry: a hybrid heterostructure wherein a ferromagnetic semiconductor Ga1−xMnxAs, with an out-of-plane component of magnetization, is cleanly interfaced with a topological insulator (Bi,Sb)2(Te,Se)3 by molecular beam epitaxy. Lateral electrical transport in this bilayer is dominated by conduction through (Bi,Sb)2(Te,Se)3 whose conductivity is a few orders of magnitude higher than that of highly resistive Ga1−xMnxAs. Electrical transport measurements in a top-gated heterostructure device reveal a crossover from weak antilocalization to weak localization as the temperature is lowered or as the chemical potential approaches the Dirac point. This is accompanied by a systematic emergence of an anomalous Hall effect. These results are interpreted in terms of the opening of a gap at the Dirac point due to exchange coupling between the topological insulator surface state and the ferromagnetic ordering in Ga1−xMnxAs. The experiments described here show that well-developed III–V ferromagnetic semiconductors could serve as valuable components of artificially designed quantum materials aimed at exploring the interplay between magnetism and topological phenomena.
AMPK-dependent phosphorylation of cingulin reversibly regulates its binding to actin filaments and microtubules
Authors: Yano Tomoki , Torisawa Takayuki , Oiwa Kazuhiro , Tsukita Sachiko 
Published: October 2018

AbstractCytoskeletal organization is essential for the precise morphogenesis of cells, tissues, and organs. Cytoskeletons, bound to scaffolding proteins, regulate the apical junction complex (AJC), which is composed of tight and adherens junctions, and located at the apical side of epithelial cell sheets. Cingulin is a tight junction-associated protein that binds to both actin filaments and microtubules. However, how cingulin binds to microtubules and whether cingulin can bind to actin and microtubules simultaneously are unclear. Here we examined the mechanisms behind cingulin’s cytoskeleton-binding properties. First, using total internal reflection fluorescence microscopy, we detected cingulin at microtubule cross points. We then found the interdomain interactions in cingulin molecules. Notably, we found that this interaction was regulated by AMPK-dependent phosphorylation and changed cingulin’s conformation and binding properties to actin filaments and microtubules. Finally, we found that the AMPK-regulated cingulin properties regulated the barrier functions of epithelial cell sheets. We propose that the cellular metabolic state, which involves AMPK, can contribute to the organization and maintenance of epithelial tissues through cingulin’s tight junction/cytoskeleton regulation.
Effects of circadian misalignment on sleep in mice
Authors: Hasan Sibah , Foster Russell G. , Vyazovskiy Vladyslav V. , Peirson Stuart N. 
Published: October 2018

AbstractCircadian rhythms and sleep-wake history determine sleep duration and intensity, and influence subsequent waking. Previous studies have shown that T cycles - light-dark (LD) cycles differing from 24 h - lead to acute changes in the daily amount and distribution of waking and sleep. However, little is known about the long-term effects of T cycles. Here we performed continuous 10 day recording of electroencephalography (EEG), locomotor activity and core body temperature in C57BL/6 mice under a T20 cycle, to investigate spontaneous sleep and waking at baseline compared with when the circadian clock was misaligned and then re-aligned with respect to the external LD cycle. We found that the rhythmic distribution of sleep was abolished during misalignment, while the time course of EEG slow wave activity (1–4 Hz) was inverted compared to baseline. Although the typical light-dark distribution of NREM sleep was re-instated when animals were re-aligned, slow wave activity during NREM sleep showed an atypical increase in the dark phase, suggesting a long-term effect of T cycles on sleep intensity. Our data show that circadian misalignment results in previously uncharacterised long-term effects on sleep, which may have important consequences for behaviour.
Intense spectrally broad-band twin beams from poled nonlinear crystals
Authors: Peřina Jan 
Published: October 2018

AbstractProperties of intense twin beams generated in parametric down-conversion in periodically poled LiNbO3 crystals and their chirped variants by intense pump fields are analyzed along the model of intense parametric down-conversion that allows for pump depletion and uses the dual spatio-spectral Schmidt modes. Spectral and spatial intensity auto- and cross-correlation functions are determined as they depend on the pump power. Temporal correlations in intense twin beams at the fs time scale are investigated using the Hong-Ou-Mandel interferometer and the process of sum-frequency generation.
Sexual conflict and the Trivers-Willard hypothesis: Females prefer daughters and males prefer sons
Authors: Lynch Robert , Wasielewski Helen , Cronk Lee 
Published: October 2018

AbstractBecause parental care is expected to depend on the fitness returns generated by each unit of investment, it should be sensitive to both offspring condition and parental ability to invest. The Trivers-Willard Hypothesis (TWH) predicts that parents who are in good condition will bias investment towards sons, while parents who are in poor condition will bias investment towards daughters because high-quality sons are expected to out-reproduce high quality daughters, while low-quality daughters are expected to out-reproduce low quality sons. We report results from an online experiment testing the Trivers-Willard effect by measuring implicit and explicit psychological preferences and behaviorally implied preferences for sons or daughters both as a function of their social and economic status and in the aftermath of a priming task designed to make participants feel wealthy or poor. We find only limited support for predictions derived from the TWH and instead find that women have strong preferences for girls and men have preferences for boys.
Cellulose defibrillation and functionalization by plasma in liquid treatment
Authors: Vizireanu Sorin , Panaitescu Denis Mihaela , Nicolae Cristian Andi , Frone Adriana Nicoleta , Chiulan Ioana , Ionita Maria Daniela , Satulu Veronica , Carpen Lavinia Gabriela , Petrescu Simona , Birjega Ruxandra , Dinescu Gheorghe 
Published: October 2018

AbstractSubmerged liquid plasma (SLP) is a new and promising method to modify powder materials. Up to now, this technique has been mostly applied to carbonaceous materials, however, SLP shows great potential as a low-cost and environmental-friendly method to modify cellulose. In this work we demonstrate the modification of microcrystalline cellulose (MCC) by applying the SLP combined with ultrasonication treatments. The plasma generated either in an inert (argon) or reactive (argon: oxygen or argon:nitrogen) gas was used in MCC dispersions in water or acetonitrile:water mixtures. An enhanced defibrillation of MCC has been observed following the application of SLP. Furthermore, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy have been applied to investigate the surface functionalization of MCC with oxygen or nitrogen moieties. Depending on the plasma treatment applied, poly (3-hydroxybutyrate) composites fabricated with the plasma modified cellulose fibers showed better thermal stability and mechanical properties than pristine PHB. This submerged liquid plasma processing method offers a unique approach for the activation of cellulose for defibrillation and functionalization, aiming towards an improved reinforcing ability of biopolymers.
CD32 Expression is not Associated to HIV-DNA content in CD4 cell subsets of individuals with Different Levels of HIV Control
Authors: García Marcial , Navarrete-Muñoz María Angeles , Ligos José M , Cabello Alfonso , Restrepo Clara , López-Bernaldo Juan Carlos , de la Hera Francisco Javier , Barros Carlos , Montoya María , Fernández-Guerrero Manuel , Estrada Vicente , Górgolas Miguel , Benito José M , Rallón Norma 
Published: October 2018

AbstractA recent study has pointed out to CD32a as a potential biomarker of HIV-persistent CD4 cells. We have characterized the level and phenotype of CD32+ cells contained in different subsets of CD4 T-cells and its potential correlation with level of total HIV-DNA in thirty HIV patients (10 typical progressors naïve for cART, 10 cART-suppressed patients, and 10 elite controllers). Total HIV-DNA was quantified in different subsets of CD4 T-cells: Trm and pTfh cells. Level and immunephenotype of CD32+ cells were analyzed in these same subsets by flow cytometry. CD32 expression in Trm and pTfh subsets was similar in the different groups, and there was no significant correlation between the level of total HIV-DNA and the level of CD32 expression in these subsets. However, total HIV-DNA level was correlated with expression of CD127 (rho = −0.46, p = 0.043) and of CCR6 (rho = −0.418, p = 0.027) on CD32+ cells. Our results do not support CD32 as a biomarker of total HIV-DNA content. However, analyzing the expression of certain markers by CD32+ cells could improve the utility of this marker in the clinical setting, prompting the necessity of further studies to both validate our results and to explore the potential utility of certain markers expressed by CD32+ cells.