bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical pregnancy after deceased donor uterus transplantation: Lessons learned and future perspectives
Authors: Chmel Roman , Pastor Zlatko , Novackova Marta , Matecha Jan , Cekal Milos , Fronek Jiri 
Published: May 2019

AbstractAimTo describe our first clinical pregnancy following a uterus transplant from a brain‐dead donor and to discuss current issues with deceased donor uterus transplantation as they relate to obstetrical success.MethodsIn August 2016, a 26‐year‐old woman with Mayer–Rokitansky–Küster–Hauser syndrome was the fourth person worldwide to receive a uterine transplant from a deceased donor and was the second in our trial. in vitro fertilization treatments using the long gonadotropin‐releasing hormone agonist protocol preceded the transplantation procedure. Frozen embryo transfers were performed in months 12, 13, 16, 19 and 23 after transplant.ResultsRecovery of the uterus of a 24‐year‐old brain‐dead nulliparous donor and the transplant procedure itself was uncomplicated. No abnormalities were revealed on Pap smears, which were performed every 6 months during the post‐transplant period, and cervical biopsies showed no epithelial dysplasia. The fifth frozen embryo transfer resulted in a clinical pregnancy. Three weeks after embryo transfer, an intrauterine gestational sac containing an embryo with a heartbeat was detected. One week later, signs of a missed abortion were revealed by ultrasound. Two weeks later, spontaneous bleeding occurred, and an ultrasound examination performed a week later confirmed an empty uterine cavity.ConclusionIn light of present research, both deceased donor uterine procurement and transplantation surgeries are technically feasible; however, more experience is needed to determine the pregnancy success rate associated with this method. Thus, additional trials of deceased donor uterine transplantation should be performed in the future to continue research related to this promising concept for the treatment of absolute uterine factor infertility.
At the crossroads of oral health inequities and precision public health
Authors: Gansky Stuart A. , Shafik Sarah 
Published: May 2019

AbstractObjectivesThis paper reviews the precision public health literature pertaining to oral health, identifies possible threats that could inadvertently increase health inequities, and proposes potential opportunities that precision public health could utilize to reduce oral health inequities.MethodsThe health sciences literature was reviewed and supplemented with new data to identify important issues relating to precision medicine, precision oral health, precision public health, and health equity.ResultsExamples from general health and oral health were provided to illustrate salient concepts.ConclusionsFuture precision public health should utilize multifactorial, multi‐level conceptual frameworks and conceptual causal models with upstream social determinants and downstream health effects, as well as a proportionate universalism perspective; and proper analytic methods, including sufficient sample sizes, appropriate statistical competitors, health disparity indices, causal modeling, and internal and external validation.
Dentist and hygienist smoking cessation counseling and awareness of Medicaid benefits
Authors: Brown Elizabeth M. , Hayes Kim A. , Olson Lindsay T. , Battles Haven , Ortega‐Peluso Christina 
Published: May 2019

AbstractObjectivesIntegrating smoking cessation interventions into dental care is an efficient way to intervene with smokers. This study of dentists and dental hygienists who provide dental care to Medicaid‐insured patients explores awareness of Medicaid smoking cessation benefits, awareness of Quitline resources, beliefs about perceived role in providing tobacco interventions, and behaviors around clinical intervention.MethodsIn 2015, we conducted a survey of dentists and hygienists who serve Medicaid patients in New York State.ResultsA total of 182 dentists and 92 hygienists completed the survey. Ninety percent reported that helping patients quit smoking is part of their role, while 51.0 percent reported feeling confident in their ability to counsel a patient about quitting. Most respondents (73.4 percent) asked patients about tobacco use, 83.7 percent advised smokers to quit, and 49.1 percent assisted with quit attempts. We found that 26.7 percent were aware that dentist smoking cessation counseling is covered by Medicaid, and 15.5 percent were aware that hygienist smoking cessation counseling is covered. A total of 38.9 percent were aware of any Medicaid coverage for smoking cessation. Awareness of the Medicaid smoking cessation benefit was associated with intervention behaviors of asking and assisting.ConclusionsMost dental care providers see smoking cessation as part of their role, but few are aware of the Medicaid benefits available to help patients. Expanding coverage of and promoting Medicaid benefits for smoking cessation have the potential to increase the reach and quality of smoking cessation interventions for Medicaid‐insured smokers, a population disproportionately affected by tobacco use.
Perceiving Revelation Here and Now: Insights from Walter Ong and Hans Urs von Balthasar
Authors: Arnold Alec 
Published: May 2019

AbstractThis essay argues that the research of Walter J. Ong, S.J. (d. 2003) is an important supplement for theological aesthetics, especially of the sort espoused by Hans Urs von Balthasar. Although Ong wrote relatively little theology proper, there is a surprising degree of confluence between Ong’s leading ideas in media/communication theory and Balthasar’s ideas about how our perception of beauty is of a piece with divine revelation. I relate their respective insights in three particular areas: (1) the phenomenology of spiritual perception; (2) the role of technology vis‐à‐vis revelation; and (3) the theology of the Word of God as word.
Analyzing the Muddles of Analysis: (Some of) What Analytic Theologians Can Learn from the History of Analytic Feminism
Authors: Rutledge Jonathan C. 
Published: May 2019

AbstractAnalytic theologians have ironically experienced difficulties in precisely defining the meaning of ‘analytic’ with respect to their style of theology. In this article, I turn to the history of a similar research project, analytic feminism, to see how it went about defining ‘analytic’ in relation to the typically non‐analytic subject area of feminist studies. I then consider two commonly referred to attempts to define analytic theology, one methodological and the other socio‐historical, and discuss shortcomings of each. I close with a new definition of analytic theology that aims to incorporate all the insights in the discussions which precede it.
Evidence of developmental escape from transcriptional gene silencing in MESSI retrotransposons
Authors: Sanchez Diego H. , Gaubert Hervé , Yang Weibing 
Published: May 2019

Summary

Transposable elements (TEs) are ubiquitous genomic features. ‘Copy‐and‐paste’ long‐terminal‐repeat (LTR) retrotransposons have been particularly successful during evolution of the plant kingdom, representing a substantial proportion of genomes. For survival in copious numbers, these TEs may have evolved replicative mobilization strategies that circumvented hosts’ epigenetic silencing. Stressful circumstances are known to trigger the majority of known mobilizing plant retrotransposons, leading to the idea that most are activated by environmental signals. However, previous research revealed that plant developmental programs include steps of silencing relaxation, suggesting that developmental signals may also be of importance for thriving parasitic elements.

Here, we uncover an unusual family of giant LTR retrotransposons from the Solanum clade, named MESSI, with transcriptional competence in shoot apical meristems of tomato. Despite being recognized and targeted by the host epigenetic surveillance, this family is activated in specific meristematic areas fundamental for plant shoot development, which are involved in meristem formation and maintenance.

Our work provides initial evidence that some retrotransposons may evolve developmentally associated escape strategies to overcome transcriptional gene silencing in vegetative tissues contributing to the host's next generation.

This implies that not only environmental but also developmental signals could be exploited by selfish elements for survival within the plant kingdom.
Brain death interval and relationship to outcomes of pediatric cardiac transplantation
Authors: Grosshuesch Craig , Johnson William K. , DeVogel Nicholas , Yin Ziyan , Wang Tao , Kindel Steven J. , Woods Ronald K. 
Published: May 2019

AbstractBackgroundWe sought to analyze brain death interval and outcomes of pediatric cardiac transplantation using national registry data.MethodsWe retrospectively evaluated a pediatric cohort from the UNOS registry from 2005 to 2014. We restricted the donor cohort to those with a primary central nervous system event as the cause of hospitalization. Brain death interval (BDI) was defined as the time between hospital admission and organ procurement. Primary outcomes were recipient and graft survival time. Logistical regression modeling was used for multivariable analysis.ResultsThe donor cohort included 2565 cases. Multivariable analysis demonstrated no relationship between BDI and recipient or graft survival time. For patient survival time, the lowest HR was 0.94 (0.63‐1.39), P = 0.531; for graft survival time, the lowest HR was 0.89 (0.53‐1.49), P = 0.563. We obtained similar results using a non‐restricted donor cohort.ConclusionsThere was no clear relationship between BDI and recipient or graft survival after pediatric cardiac transplantation.
A new approach to estimate aerobic fitness using the NHANES dataset
Authors: Lu Kim D. , Bar‐Yoseph Ronen , Radom‐Aizik Shlomit , Cooper Dan M. 
Published: May 2019

IntroductionPhysical activity and fitness are essential for healthy growth in children. The National Health and Nutrition Examination Survey (NHANES) evaluated fitness by estimating V̇O2max from submaximal measurements of heart rate (HR) during graded treadmill exercise. Our aims were (a) to examine how well NHANES methodology used to estimate V̇O2max correlated with actual VO2max and (b) to evaluate a novel fitness metric using actual data collected during exercise and its relationship to physical activity and sedentary time, lipid profiles, and body composition.MethodsFifty‐three adolescents completed NHANES submaximal exercise protocol and maximal graded cardiopulmonary exercise testing. We used a novel approach to quantifying fitness (Δvelocity × incline × body mass (VIM)/ΔHR slopes) and evaluated its relationship to physical activity and sedentary time using NHANES data (n = 4498). In a subset (n = 740), we compared ΔVIM/ΔHR slopes to NHANES estimated V̇O2max and examined their relationship to cardiovascular risk factors (BMI percentiles and lipid levels).ResultsMeasured V̇O2peak was moderately correlated with NHANES estimated V̇O2max (r = 0.53, P < 0.01). Significantly higher ΔVIM/ΔHR slopes were associated with increased physical activity and decreased sedentary time. ΔVIM/ΔHR slopes were negatively associated with LDL, triglycerides, and BMI percentiles (P < 0.01). In general, the two fitness models were similar; however, ΔVIM/ΔHR was more discriminating than NHANES in quantifying the relationship between fitness and LDL levels.ConclusionWe found that the NHANES estimated V̇O2max accounted for approximately 28% of the variability in the measured V̇O2peak. Our approach to estimating fitness (ΔVIM/ΔHR slopes) using actual data provided similar relationships to lipid levels. We suggest that fitness measurements based on actually measured data may produce more accurate assessments of fitness and, ultimately, better approaches linking exercise to health in children.
“Shifting the Power”: Youth Activists’ Narrative Reframing of the Immigrant Rights Movement
Authors: Cabaniss Emily R. 
Published: May 2019

This article explores collective efforts by undocumented youth activists to use storytelling to reframe the debates around immigration reform and discursively position themselves as the rightful leaders of a movement that had been dominated by adult citizen‐advocates. Drawing on 19 months of fieldwork, 37 in‐depth interviews, and hundreds of pages of movement documents, I show how youth activists in the United States worked together to develop stories that: (1) drew into question the legitimacy of adult citizen‐advocates to speak on issues of immigration and (2) cast undocumented immigrant youth as the proper authorities on these matters. I argue that through collective storytelling and character work, the activists were able to subvert adult citizen authority and construct themselves as powerful, new collective actors in the contemporary immigrant rights movement. I conclude by discussing some of the practical implications and limitations of using narrative reframing strategies to advance the social change agendas of marginalized movement factions.
The Hybrid Theory of Stories and Arguments Applied to the Simonshaven Case
Authors: Bex Floris J. 
Published: May 2019

AbstractThis paper presents the hybrid theory of stories and arguments for reasoning with evidence in legal cases and applies this theory to the Simonshaven case. In the hybrid theory, alternative hypothetical stories about “what happened” in a case are constructed and discussed in a dialectical process of argument and counterargument. After informally explaining stories, arguments, and the ways in which they interact, this paper gives a method for rational proof based on critical questions and shows how this method can be used in the Simonshaven case.
A predicted plastid rhomboid protease affects phosphatidic acid metabolism in Arabidopsis thaliana
Authors: Lavell Anastasiya , Froehlich John E. , Baylis Olivia , Rotondo Anthony D. , Benning Christoph 
Published: May 2019

SummaryThe thylakoid membranes of the chloroplast harbor the photosynthetic machinery that converts light into chemical energy. Chloroplast membranes are unique in their lipid makeup, which is dominated by the galactolipids mono‐ and digalactosyldiacylglycerol (MGDG and DGDG). The most abundant galactolipid, MGDG, is assembled through both plastid and endoplasmic reticulum (ER) pathways in Arabidopsis, resulting in distinguishable molecular lipid species. Phosphatidic acid (PA) is the first glycerolipid formed by the plastid galactolipid biosynthetic pathway. It is converted to substrate diacylglycerol (DAG) for MGDG Synthase (MGD1) which adds to it a galactose from UDP‐Gal. The enzymatic reactions yielding these galactolipids have been well established. However, auxiliary or regulatory factors are largely unknown. We identified a predicted rhomboid‐like protease 10 (RBL10), located in plastids of Arabidopsis thaliana, that affects galactolipid biosynthesis likely through intramembrane proteolysis. Plants with T‐DNA disruptions in RBL10 have greatly decreased 16:3 (acyl carbons:double bonds) and increased 18:3 acyl chain abundance in MGDG of leaves. Additionally, rbl10‐1 mutants show reduced [14C]–acetate incorporation into MGDG during pulse−chase labeling, indicating a reduced flux through the plastid galactolipid biosynthesis pathway. While plastid MGDG biosynthesis is blocked in rbl10‐1 mutants, they are capable of synthesizing PA, as well as producing normal amounts of MGDG by compensating with ER‐derived lipid precursors. These findings link this predicted protease to the utilization of PA for plastid galactolipid biosynthesis potentially revealing a regulatory mechanism in chloroplasts.
Structure of membrane tethers and their role in fusion
Authors: Ungermann Christian , Kümmel Daniel 
Published: May 2019

AbstractVesicular transport between different membrane compartments is a key process in cell biology required for the exchange of material and information. The complex machinery that executes the formation and delivery of transport vesicles has been intensively studied and yielded a comprehensive view of the molecular principles that underlie the budding and fusion process. Tethering also represents an essential step in each trafficking pathway. It is mediated by Rab GTPases in concert with so‐called tethering factors, which constitute a structurally diverse family of proteins that share a similar role in promoting vesicular transport. By simultaneously binding to proteins and/or lipids on incoming vesicles and the target compartment, tethers are thought to bridge donor and acceptor membrane. They thus provide specificity while also promoting fusion. However, how tethering works at a mechanistic level is still elusive. We here discuss the recent advances in the structural and biochemical characterization of tethering complexes that provide novel insight on how these factors might contribute the efficiency of fusion.
A case of hypercalcemia in a heifer presumably due to mammary carcinoma bovine hypercalcemia of malignancy
Authors: Varvil Mara S. , Haanen Gillian , Sola Mario F. , Lin Tsang , Kritchevsky Janice E. , Christian John A. 
Published: May 2019

AbstractMammary carcinoma is rare in cattle with only a handful of cases found in the literature, and none have reported an associated hypercalcemia. An 8‐year‐old Holstein‐Friesian heifer was presented to the Purdue University Veterinary Teaching Hospital's Large Animal Hospital with a 3‐month history of lethargy. Laboratory abnormalities included ionized hypercalcemia and hypophosphatemia (2.28 mmol/L and 1.8 mg/dL, respectively). Physical examination revealed a mammary mass that was cytologically described as a suspected mammary carcinoma, which was later confirmed by histologic evaluation. On surgical removal of the mass, calcium initially decreased rapidly, and within a few days was within the RI, further supporting a diagnosis of hypercalcemia of malignancy in a heifer. However, attempts to confirm this using hormone profiles (parathyroid hormone [PTH], parathyroid hormone‐related protein [PTHrp], and calcitriol) were inconclusive due to the lack of validated assays and RIs for cattle. Immunohistochemical staining for PTHrP showed scattered cytoplasmic staining among the neoplastic cells, suggesting PTHrP production by these cells.
Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay
Authors: Giordano Liliana , Fiori Barbara , D’Inzeo Tiziana , Parisi Gabriella , Liotti Flora Marzia , Menchinelli Giulia , De Angelis Giulia , De Maio Flavio , Luzzaro Francesco , Sanguinetti Maurizio , Posteraro Brunella , Spanu Teresa 
Published: May 2019

We directly tested 484 organisms from clinical (
n
= 310) and simulated (
n
= 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing
Enterobacterales
detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives.
Evaluation of the iPACK block injectate spread: a cadaveric study
Authors: Tran John , Giron Arango Laura , Peng Philip , Sinha Sanjay Kumar , Agur Anne , Chan Vincent 
Published: May 2019

Background and objectivesUltrasound-guided infiltration of the interspace between the popliteal artery and capsule of the knee (iPACK) block, a new regional analgesic technique, is believed to relieve posterior knee pain, after total knee arthroplasty, by targeting the articular branches innervating posterior aspect of the joint. The extent of injectate spread and the number of articular branches affected is currently unknown. This cadaveric study aimed to compare the area of dye spread and frequency of articular branches staining following a proximal versus distal injection technique.MethodsAn ultrasound-guided iPACK injection (10 mL of methylene blue dye solution) was performed in 14 lightly embalmed specimens: 7 injected using a proximal injection technique (1 fingerbreadth above base of patella) and 7 using a distal injection technique (at the superior border of the femoral condyles). Following injection, dissection, digitization, and 3D modeling were performed to map the area of dye spread and determine the frequency of nerve staining.ResultsBoth injection techniques achieved a similar mean area of injectate spread. Of the four articular branches supplying the posterior knee joint capsule, the genicular branch of posterior division of obturator nerve was stained in all specimens. The proximal injection resulted in staining of superior medial genicular nerve, due to dye spread through the adductor hiatus, whereas superior lateral genicular nerve and anterior branch of common fibular nerve were consistently stained following distal injection. Other articular branches were stained with variable frequency.ConclusionsBoth proximal and distal iPACK injection techniques provided a similar area of dye spread in the popliteal region and extensive staining of the articular branches supplying the posterior capsule. The proximal injection technique promoted greater anteromedial dye spread, while the distal injection had more anterolateral spread. Further clinical study is required to confirm our cadaveric findings.
The impact of implementation of a national smoke-free prisons policy on indoor air quality: results from the Tobacco in Prisons study
Authors: Semple Sean , Dobson Ruaraidh , Sweeting Helen , Brown Ashley , Hunt Kate 
Published: May 2019

ObjectiveTo determine secondhand smoke (SHS) concentrations in prisons during the week of implementation of a new, national prisons smoke-free policy.DesignRepeated measurement of SHS concentrations immediately before and after implementation of smoke-free policies across all 15 prisons in Scotland, and comparison with previously gathered baseline data from 2016.MethodsFine particulate matter (PM2.5) measurements at a fixed location over a continuous 6-day period were undertaken at the same site in each prison as previously carried out in 2016. Outdoor air quality data from the nearest local authority measurement station were acquired to determine the contribution of outdoor air pollution to indoor prison measurement of PM2.5.ResultsAir quality improved in all prisons comparing 2016 data with the first full working day postimplementation (overall median reduction −81%, IQR −76% to −91%). Postimplementation indoor PM2.5 concentrations were broadly comparable with outdoor concentrations suggesting minimal smoking activity during the period of measurement.ConclusionsThis is the first evaluation of changes in SHS concentrations across all prisons within a country that has introduced nationwide prohibition of smoking in prisons. All prisons demonstrated immediate substantial reductions in PM2.5 following policy implementation. A smoke-free prisons policy reduces the exposure of prison staff and prisoners to SHS.