bluuebunny/crossref_metadata_2025_split huggingface dataset:
PL - 027 Up-regulation of NRG1 improves cardiac repair in zebra fish and involved in the cardioprotective effects of exercise training in rats of myocardial infarction
Authors: Cai Mengxin , Du Shaojun , Tian Zhenjun 
Published: October 2018

Objective Myocardial infarction (MI) remains a leading cause of morbidity and mortality worldwide. Exercise training could improve cardiac function following MI. However, the mechanisms are still not well-known. Neuregulin 1 (NRG1)plays an important role in heart development and regeneration.In this study, we investigated the effect of NRG1 on cardiac regeneration in a zebrafish model, detected whether exercise could improve cardiac function through regulating NRG1 expression in infarcted heart and explore the possible role of up-regulation of NRG1 in skeletal muscle play in the cardioprotective effects in rats with MI.
Methods Transgenic zebrafish line, cmlc2:CreERandβ-act2:BSNrg1,wereusedto study the effect of NRG1 on heart growth and regeneration after injury. PCNA was detected by immunofluorescence staining andmRNAexpression of gata4, nkx2.5, tbx5, smyd1b, hsp90α and murf were tested by RT-PCR.Sprague-Dawley rats were used to establish MI model and underwent fourweeks of exercise training (ET) or pAAV-{dMCK promoter}rNRG1-eGFP intervention.AG1478 was used asan inhibitor of NRG1/ErbBs signaling pathway. Cardiac function and structure,cardiomyocyte proliferation and NRG1 expression were detected in the heart or skeletal.
Results Cardiac-specific overexpression of NRG1 induced cardiac hypertrophy and cardiomyocyte proliferation, regulated the mRNA expression of gata4, nkx2.5, tbx5, smyd1b, hsp90α andmurf in uninjuriedzebrafish, and promote cardiac repair and regeneration after injury in the zebrafish.Exercise activated NRG1/ErbBs signaling pathway, improved cardiac remodeling and heart function, enhanced cardiomyocyte proliferation, reduced cardiomyocyte apoptosis, ROS level and MuRF1 protein expression in rats with MI. BlockingErbB signaling attenuated the ET-induced cardioprotection effects in rat with MI.up-regulation of NRG1 expression in skeletal muscle could increase the protein level of NRG1 in serum and infarcted heart, improve cardiomyocyte proliferation and reduce the level of cardiac fibrosis, finally promote cardiac function.
Conclusions Up-regulation of NRG1 expression in the heart or skeletal musclemay be one of the underlying mechanisms of thebeneficial effects of exercise training following MI.
PL - 038 Habitual swimming exercise induced partial resistance to rat Alzheimer's disease
Authors: Cheng-Yi Liu Timon , Zhang Quan-Guang , Wu Chong-Yun , Yang Luo-Dan , Zhu Ling , Duan Rui 
Published: October 2018

Objective In MSSE, we have divided male 2.5-month-old Sprague-Dawley rats into the following 4 groups: control (C), habitual swimming (SW), Alzheimer’s disease (AD) induction without swimming (AD), and habitual swimming and then AD induction (SA), and found the perfect resistance of habitual swimming to AD induction by using the P value statistics of the 5 behavior parameters of rats and the 23 physiological and biochemical parameters of their hippocampus. The topological difference of four groups were further calculated in this paper by using quantitative difference (QD) and self-similar approach.
Methods 1. The logarithm to base golden section τ (lt) is called golden logarithm. It was found that σ=ltσ ≈ 0.710439287156503. 2. For a process from x1 to x2, lx(1,2)=lt(x2/x1) and its absolute vale are called the process logarithm and its QD, QDx(1,2). There are QD threshold values (αx,βx,γx) of function x which can be calculated in terms of σ. The function x is kept to be constant if QDx(1,2) < αx. A function in/far from its function-specific homeostasis is called a normal/dysfunctional function. A normal function can resist a disturbance under its threshold so that QDx(1,2) < βx. A dysfunctional function is defined as the QD is significant if βx ≦QDx(1,2) < γx and extraordinarily significant if QDx(1,2) ≧ γx. 3. Self-similarity was studied in the fractal literature: a pattern is self-similar if it does not vary with spatial or temporal scale. First-order self-similarity condition leads to the power law between two data sets A = {xi} and B = {yi}; yi = ai xi if the QDi of ai and the average of {ai} is smaller than βmin=min{βi} and the average QD of {QDi} is smaller than αmin=min{αi}. 4. The σ algorithm for integrative biology was established based on high-order self-similarity. Those parameters that contribute to the topological difference were the biomarkers.
Results The 28 dimension data set consisted of all the 28 parameters. The first-order self-similarity held true for the 28 dimension data sets between groups C and SW. The topological algorithm of other groups suggested three AD biomarkers, protein carbonyl, granules density of presynaptic synaptophysin in the hippocampal CA1 and malondialdehyde intensity. The first two biomarkers were completely reversed by exercise pretreatment, but the third biomarker was partially reversed.
Conclusions Exercise pretraining exerts partial benefits on AD that support its use as a promising new therapeutic option for prevention of neurodegeneration in the elderly and/or AD population.
OR-019 VEGF-B inhibits skeletal muscle apoptosis after exercise in Chronic heart failure rats
Authors: Li Lingjie , Zhang Jing , Chen Xuefei 
Published: October 2018

Objective To investigate the effects of vascular endothelial growth factor B in aerobic exercise mediated chronic heart failure rats cardiac function improvement and skeletal muscle remodeling.
Methods We employed transverse abdominal aortic constriction (TAC) inducing CHF in Sprague Dawley rats. Controls were sham-operated animals. At 4 weeks after surgery, rats were randomized to 4 weeks of aerobic exercise (CHF+E) or to untrained groups (CHF). After 8 weeks, all rats went echocardiography test. After which, rats were sacrificed and samples were collected. Muscular cytokine VEGFB and its receptor NRP1 expression were analyzed. Expression of apoptosis and muscle atrophy markers were assessed in cardiac muscle、gastrocnemius.
Results TAC rats developed CHF (preserved LV ejection fraction, hypertrophy of myocardial cells, decreased FS, increased LVAW d and LVID s). Exercise ameliorate CHF rat cardiac function. TAC rat skeletal muscle developed irregular muscle fiber distribution.The two atrophy-related ubiquitin ligases atrogin-1 and MuRF1, as well as genes involved in apoptosis and autophagy were upregulated in muscles in CHF rats. Exercise inhibited muscle atrophy and skeletal muscle apoptosis.VEGFB and its receptor NRPI decreased significantly in CHF muscle. Exercise promoted VEGFB and NRP1 expression in cardiac tissue, gastrocnemius.
Conclusions Exercise ameliorates CHF rat cardiac function. VEGFB inhibits cardiac muscle and gastrocnemius apoptosis in CHF rats.
OR-021 GLUT4 rs5418 genotype and performance of Cross-country skiers in China
Authors: Li Yanchun , Ren Hong , Hu Yang , Mei Tao 
Published: October 2018

Objective The GLUT4 gene is one of the genes that have a potential influence on physical performance. Studies have shown that the rs5418 genotype of GLUT4 is more prevalent in endurance athletes. Therefore, the GLUT4 rs5418 polymorphism may become a genetic marker for Cross-country skiers. The study aimed to examine the association of the GLUT4 rs5418 genotype with the performance of Cross-country skiers.
Methods The distributions of the GLUT4 rs5418 genotype and allele were examined in a general population (206) and a group of elite Cross-country skiers (163) in China by using PCR-RFLP and TOF.
Results Compared with the general population, the elite Cross-country skiers (χ2=9.267；df=2；P=0.01；P<0.05), especially the females, had a higher frequency of the AA genotype(Total: 22.09% VS 13.59%, Female:24.19% VS 13.59% ). The Cross-country skiers had a higher frequency of the A allele than the general population(45.40% VS 33.98%), and the difference was statistically significant (χ2=9.972；df=1；P=0.02；P<0.05).
Conclusions The GLUT4 rs5418 polymorphism was associated with the performance of elite Cross-country skiers in China. The SNP rs5418 could be used as a biomarker for selecting elite Cross-country skiers in China.
OR-037 The Effect of Exercise on Inflammatory Factors in Breast Cancer Patients: A Meta-analysis
Authors: Qiu Lingling , Xu Ya li , Kong Song hu , Zhao Jie xiu 
Published: October 2018

Objective Breast cancer is one of the most common malignant tumors that threaten the physical and mental health and even life-threatening of women worldwide.Chronic inflammation plays a key role in the occurrence, progression and recurrence of cancer.Several sources of evidence indicate that exercise during and after breast cancer could positively modulate the tumor microenvironment. The purpose of this meta-analysis is to determine the impact of exercise training on inflammatory factors in breast cancer patients.
Methods We systematically searched the relevant randomized controlled trials published from January 1990 to June 2017 and analyzed them by Pubmed、Embase、Web of Science and Cochrane Library.The combination of Breast Cancer and Exercise is used for retrieval.The search term used for Pub Med is:（Exercise[Me SH] OR Physical Activity[Text Word] OR Training [Text Word] OR Activity [Text Word] OR Sport[Text Word] AND Breast Cancer[Text Word] OR Breast Tumor [Text Word] OR Breast Neoplasm [Text Word] OR Mammary Cancer [Text Word] OR Malignant Neoplasm of Breast [Text Word] OR Breast Carcinoma [Text Word] AND Interleukins [Text Word] OR IL [Text Word]）.And manually check the references in the article to identify additional articles. Examination of titles and abstracts of papers based on pre-set inclusion criteria.Eleven high-quality trials were included.
Results Pooled analyses revealed compared with the control group, the exercise group significantly improved the serum concentration of IL-8(Z=0.07,SMD=-0.02, 95%CI:[-0.47,-0.44],p=0.946)and TNF-α(Z=2.10, SMD=-0.60,95%CI:[-1.16,-0.04],p=0.036). No significant differences were found in the serum concentrations of IL-2(Z=1.96,SMD=-0.69,95%CI:[-1.37,0.00],p=0.05),IL-6(Z=0.40,SMD=-0.12, 95%CI: [-0.69,0.45],p=0.686),IL-10 (Z=1.73,SMD=-0.45,95%CI:[-0.95,0.06],p=0.084)or C-reactive protein(Z=0.18, SMD=-0.03,95%CI:[-0.35,0.41],p=0.861).
Conclusions Exercise training can effectively improve some inflammatory factors in breast cancer patients and may affect tumor microenvironment. These findings provide a theoretical basis for the promotion of sports in this population.
OR-050 The Development of a Protein Powder Product with an integrated solution for Active Population
Authors: Cheng Junrui , Zhang Sophia , Zhang Sophia , Du Eric 
Published: October 2018

Objective Sports nutrition is expanding from professional athletes to a broader spectrum of consumers focused on fitness and improving overall wellness. The recommendation of combining exercise and healthy diet has been highlighted by Health China 2030 and National Nutrition Planning, leading to increased embrace of active lifestyle among Chinese people. Along with the increasing involvement in sports comes with concerns on decreasing resting metabolic rate due to weight loss, impaired joint health and post-exercise immunological response. To confront the above-mentioned challenges, we aimed to develop a product with an integrated solution to support fitness goals while diminishing exercise-related concerns.
Methods Literature review was conducted for core ingredient screen, followed by in vitro studies to substantiate and compare efficacy of ingredients from various resources. Muscle protein synthesis was measured in myotubes during the differentiation and fusion of C2C12 myocytes. Myotube fusion density was quantified by ImageJ, an image processing program, subsequent to myotube staining.
Results Muscle mass gain enhances resting metabolic rate. It has been shown that whey protein supplementation at 20 g/d significantly increased lean muscle synthesis rate in post-exercise subjects. The efficacy of whey protein powder sourced from different resources were compared by conducting in vitro studies. A large body of evidence has shown that collagen peptide supplementation can mitigate joint pain and maintain joint health in athletic population. Yeast β-glucan was spotlighted as an immunity enhancer with antimicrobial protection and benefit in minimizing post-exercise immunosuppression.
Conclusions A protein powder containing over 20 g/serving whey protein, combining with collagen peptide and yeast β-glucan may convey beneficial effects in muscle synthesis, as well as protective effects against joint pain and post-exercise immunosuppression among active population. The combination of the above-mentioned ingredients could provide a well-round solution to confront the challenges that met by most active population.
OR-052 Effects of Exercise Intervention on Cancer-Related Fatigue in Breast Cancer Survivors
Authors: Xie Zhiling , Ye Weibing 
Published: October 2018

Objective cancer-related fatigue(CRF) is the most commonly reported and most distressing symptom in cancer patient.The purpose of this study was to review the effect of exercise intervention on cancer-related fatigue in breast cancer survivors.
Methods From 1998 to 2018 in Chinese and English literature of Wanfang Database, Pubmed, SportDiscus and Springer databases, picked out the randomized controlled trials which up to standard. Keywords cancer, exercise, fatigue, etc. were used for systematic search and tracking. 12 experiments were reviewed to analyze the effect differences between exercise intervention and exercise-related fatigue.
Results Most interventions use aerobic exercise as the primary form of exercise. Generally, exercise interventions are effective for cancer-related fatigue, but some findings are not. Many studies have shown that moderate intensity aerobic exercise has a significant effect on cancer-related fatigue, with 50-70% heart rate reserve and 3-5 times of exercise per week for at least 30 minutes each time. Exercise methods mainly include walking, yoga, cycling and tai chi. Firstly, Supervised aerobic exercise was statistically more effective than conventional care in improving CRF among breast cancer survivors. It has been shown that group-based, supervised exercise produces positive psychosocial‘side-effects’ due to social interactions, improved self-efficacy, and attention from a trainer. Secondly, Cancer fatigue is divided into acute and chronic fatigue, 18 weeks of exercise intervention can reduce the short-term fatigue, at 36 weeks, baseline levels of fatigue index responses and contrast on the issue of the multivariate statistics. Thirdly, usual-care group were reported that they had been actively engaged in regular exercise before study enrollment. During the exercise intervention, most studies on the control ways are according to the daily life or to take care of, but studies have reported, before intervention, to a high level of 40% in the control group often exercise, exercise also as usual during the intervention, which causes the control to the baseline level is higher, but the intervention group and control group will be difference, no significant difference, lead to the result is invalid.
Conclusions First, the exercise intervention of cancer-related fatigue needs to be supervised; second, the exercise intervention is effective for short-term cancer-related fatigue; third, the daily exercise level of the control group will affect the intervention effect.
PO-035 Association between sedentary behavior and diabetes mellitus in the prevalence of middle-aged and elderly people
Authors: Wang Xinzheng , Luo Hongbin 
Published: October 2018

Objective This study aims to explore the relationship between sedentary behavior and the diabetes mellitus prevalence of middle-aged and elderly people.
Methods we conduct a questionnaire survey and physical examination for a total of 3,000 middle-aged and elderly people (≥45 years old) ,analysing by he software of SPSS21.0 and Stata12.0.
Results The logistic regression analysis shows that the risk of diabetes is 1.617 (95% CI, 0.762-1.789, P <0.05) at 2-4h, 4-6h, 6-8h, ≥8h, = 0.003), 1.235 (95% CI, 0.818-1.865, P = 0.034), 3.420 (95% CI, 2.241-5.218, P = 0.000), 5.014 (95% CI, 3.049-8.247, P = 0.000). With each additional one-hour sedentariness the risk of diabetes increases by 23% (OR1.23, 95% CI 1.18-1.29, p <0.0001).
Conclusions The sedentary behavior is an independent risk factor for diabetes. The prevalence of diabetes is gradually increasing with the increase of sedentary time, which indicates the longer sedentary time, the higher prevalence of diabetes.
PO-044 Effects of one-time high intensity intermittent training on the expression of UCP1 mRNA in adipose tissue of obese rats
Authors: Li Yafei , Liang Chunyu , Zhou Qishu , Yan Yi 
Published: October 2018

Objective To observe the effect of one-time high-intensity intermittent training (HIIT) on the white fat browning in obese rats.
Methods 12 obese male rats at the age of 19-week were randomly divided into control group (OC group, n=6) and HIIT group (n=6). OC group doesn’t do exercise, and HIIT group finish the once high-intensity intermittent training ( ①Warm up: 70%VO2max, 7min; ②High intensity exercise: 90%VO2max, 3min+ 50%VO2max, 3min, 6 times; ③Convalescence: 70%VO2max, 7min.). The maximal oxygen uptake (VO2max) of HIIT group was tested before training, and the exercise intensity was determined according to the average VO2max. The subcutaneous and testicle white adipose tissue (WAT) and brown adipose tissue (BAT) of the scapula were taken immediately after exercise. The expression of UCP1 mRNA in the WAT and BAT using the fluorescent quantitative PCR.
Results Compared with the OC group, the expression of BAT UCP1 mRNA , in the HIIT group was increased, but there was no significant difference (1.40±0.50 vs 1.03±0.27, P>0.05). In both OC and HIIT group, the expression of UCP1 mRNA of the subcutaneous and testicle WAT were too low to test. The results showed that one-time HIIT exercise had no effect on the expression of UCP1 mRNA in WAT.
Conclusions The effects of One-time HIIT on the expression of UCP1 mRNA in adipose tissue of obese rats are still not definite.
PO-064 A potential role of stretch-activated channels in anabolic mechanotransduction in the atrophied rat soleus muscle
Authors: Mirzoev Timur , Tyganov Sergey , Shenkman Boris 
Published: October 2018

Objective Prolonged immobilization or unloading of skeletal muscle causes muscle disuse atrophy, which is characterized by a reduction in muscle cross-sectional area and compromised contractile function. To date, the mechanisms of anabolic mechanotransduction in the atrophied mammalian skeletal muscle remain poorly understood. The aim of the present study was to assess a possible role of stretch-activated ion channels (SAC) in the propagation of a mechanical signal to anabolic signaling and protein synthesis (PS) in an isolated rat soleus muscle following mechanical unloading.
Methods The mechanical unloading was performed via hindlimb suspension (HS). Twenty-eight male Wistar rats weighing were randomly assigned to the following 4 groups (n=7/group): 1) vivarium control (C), 2) control + SAC inhibitor (gadolinium) (C+Gd3+), 3) 7-day HS (HS), 4) 7-day HS + SAC inhibitor (HS+Gd3+). Following unloading, an isolated rat soleus was placed in an organ culture medium and subjected to a bout of eccentric contractions (EC). Upon completion of the EC, muscles were collected for Western blot analyses to determine the content of the key anabolic markers. The rate of PS was measured by SUnSET technique.
Results EC-induced increase in PS was significantly less in the HS and HS+ Gd3+ groups vs. the C group. There was no statistically significant difference between the HS and HS+ Gd3+ groups in terms of EC-induced increase in muscle PS. A decrease in EC-induced phosphorylation of p70S6K, 4E-BP1, RPS6 and GSK-3beta in the 7-day unloaded soleus treated with SAC inhibitor did not differ from that of the 7-day unloaded soleus without SAC blockade. Thus, the inhibition of SAC with gadolinium did not lead to further decline in EC-induced phosphorylation of the key anabolic markers and muscle PS.
Conclusions The results of the study suggest that attenuation of mTORC1-signaling and PS in response to EC in unloaded soleus muscle may be associated with inactivation of SAC. The study was supported by the RFBR grant # 16-34-60055.
PO-069 Treadmill exercise reduces Aβ deposition in the hippocampus of APP/PS1 mice by activating AMPK
Authors: Yan Qingwei , Xu Bo 
Published: October 2018

Objective To investigate the molecular mechanism of AMPK-mediated motion reduction of Aβ deposition in the hippocampus of APP/PS1 mice.
Methods The 3 months of C57BL/6 48 APP/PS1 mice and 48 wildtype mice were randomly divided into APP/PS1 quiet control group (ADC, n=24) and exercise group (ADE, n=24), wildtype quiet control group (the WTC, n=24) and exercise group (WTE, n=24).ADE and WTE groups performed 12 weeks of treadmill exercise, while ADC and WTC groups were placed in a quiet running platform environment. The contents of ATP, AMP in hippocampus were tested by ELISA, AMPK and BACE1 mRNA in hippocampus were tested by RT-qPCR, Protein expression of AMPK, Sirt1, PPARγ, PGC1α, BACE1, Aβ in hippocampus were tested by Western Blot, the hippocampus SPs were tested by immunohistochemical experiments.
Results 12 weeks treadmill exercise can improve the AMP、ATP content, and the AMP/ATP ratio (p<0.05) in APP/PS1 mice hippocampus, up-regulation the mRNA and protein phosphorylation of AMPK(p<0.05),the protein expression of Sirt1、PPARγ and PGC1α(p<0.05)in APP/PS1 mice hippocampus, down-regulation the BACE1mRNA and protein expression levels (p<0.05), Aβ protein and SPs level (p<0.01) in APP/PS1 mice hippocampus.
Conclusions The molecular mechanism of decrement Aβ deposition in APP/PS1 mice hippocampus mediated by 12 weeks of treadmill exercise may be related to activation of AMPK and the improvement of hippocampus energy metabolism by exercise, and then regulatiing the AMPK-BACE1 pathway.