bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is low‐dose glucagon needed and effective in preventing fasted exercise‐induced hypoglycaemia in type 1 diabetes treated with the MiniMed 780G, an automated insulin delivery system?
Authors: Lundemose Sissel Banner , McCarthy Olivia M. , Christensen Merete Bechmann , Laugesen Christian , Bracken Richard M. , Holst Jens Juul , Ranjan Ajenthen Gayathri , Nørgaard Kirsten 
Published: November 2024

AbstractAimTo evaluate and compare the plasma glucose (PG) response during spontaneous fasted morning moderate‐intensity exercise with and without injection of subcutaneous glucagon in adults with type 1 diabetes (T1D) treated with an automated insulin delivery (AID) system.MethodsTen adults (four female) with T1D (age 50 [42–67] years, diabetes duration: 22 [14–44] years, HbA1c: 55 [47–69] mmol/mol) treated with the MiniMed™ 780G AID system participated in a proof‐of‐concept two‐period, crossover trial. Fasting participants undertook a 45 min bout of continuous moderate‐intensity (~60% V̇O2peak) exercise on a cycle ergometer followed by 1 h of rest. Before exercise, 150‐μg glucagon was administered subcutaneously on visit 1 (GLUC) but not on visit 2 (NO‐GLUC). Temporary target on the AID was activated 15 min before until 15 min after exercise cessation. Blood samples were taken at 5‐ and 15‐min intervals for measuring PG and biomarkers. Data were analysed using paired t tests or repeated measures ANOVA.ResultsTime in range (3.9–10.0 mmol/L) was 100% on both study visits. No hypoglycaemia (<3.9 mmol/L) occurred in either arm. The GLUC arm had significantly higher mean PG (p = 0.01), area under the PG curve (p = 0.01), coefficient of variation (p < 0.01), peak PG (p = 0.01) and PG at the end of exercise (p < 0.01). No differences in endogenous glucoregulatory hormones were observed between visits.ConclusionAdults with T1D treated with the MiniMed™ 780G can perform spontaneous fasted moderate‐intensity exercise without hypoglycaemia. Therefore, glucagon was not needed for prevention of hypoglycaemia in such situations.
The effect of altered sleep timing on glycaemic outcomes: Systematic review of human intervention studies
Authors: Slebe Romy , Splinter Jane J. , Schoonmade Linda J. , Blondin Denis P. , Campbell David J. T. , Carpentier André C. , Després Jean‐Pierre , Hoeks Joris , Kalsbeek Andries , Raina Parminder , Schrauwen Patrick , Serlie Mireille J. , Stenvers Dirk Jan , Yi Chun‐Xia , de Mutsert Renée , Beulens Joline W. J. , Rutters Femke 
Published: November 2024

AbstractAimsAlterations in sleep timing can lead to disturbances in glycaemic control, although the evidence is inconsistent. Therefore, this systematic review summarizes results from human intervention studies of altered sleep timing on glycaemic outcomes.Materials and MethodsAs part of a broader search on the effect of altering timing of sleep, physical activity and dietary intake, Medline and Embase were searched from inception to February 2023, and subsequent reference searches were done. With the help of a machine learning–aided program ‘ASReview’, we selected any type of intervention study in the general adult population, which acutely delayed sleep by ≥2 h for at least one night, while the total time in bed was the same between early and late sleep. Quality assessment was done using the quality assessment tool for quantitative studies.ResultsIn total, 14 studies (159 adults with normal or increased weight) were identified. Methodological quality was high (n = 4), moderate (n = 7) or low (n = 3). Acute delays of sleep onset showed unfavourable effects in 10 out of 27 measured glycaemic outcomes (one–six studies reported on each outcome) with outcomes mostly measured in the postprandial period, compared to (early) nighttime sleep.ConclusionsAcutely delaying sleep timing might have unfavourable effects on glycaemic outcomes, compared to (early) nighttime sleep. Future research does however need better controlled trials, also measuring and controlling sleep quantity, sleep quality, physical activity and dietary intake, with longer follow‐up periods, consistent outcomes and designs and more diverse populations to provide targeted advice regarding the optimal timing for sleep.Protocol registrationThis review is part of a larger search ‘The effect of altering timing of physical activity, sleep and energy intake on glycaemia and Type 2 Diabetes risk in humans’, of which the protocol was registered in the PROSPERO database on 27 November 2021 under number: CRD42021287828.
Expert opinions on pharmacological symptomatic treatment of behavioral symptoms in frontotemporal dementia: A survey of the Neuropsychiatric International Consortium on Frontotemporal Dementia (NIC‐FTD)
Authors: van Paassen Dirk , Hartog Luc , de Boer Sterre , Vijverberg Everard , Ducharme Simon , Pijnenburg Yolande , Santillo Alexander 
Published: November 2024

AbstractBackground and purposeBehavioral variant frontotemporal dementia (bvFTD) is essentially characterized by progressive changes in personality and cognition. Clinically, bvFTD presents with often profound behavioral symptomatology. Despite the high burden of these symptoms for both patients and caregivers, there is no general consensus on an effective pharmacological symptomatic treatment. Interestingly, for multiple similar symptoms in primary psychiatric disorders, there is consensus on an effective pharmacological treatment. The aim of this study is to explore currently preferred clinical practices in the pharmacological treatment of specific core behavioral symptoms in bvFTD by world‐leading clinical experts.MethodsA digital survey was conducted among members of the Neuropsychiatric International Consortium on Frontotemporal Dementia, comprising neurologists, psychiatrists, and neuropsychiatrists. Respondents recommended pharmacological treatments targeting symptoms including disinhibition, apathy, loss of empathy, hyperorality, perseverative/compulsive behaviors, and positive psychotic symptoms.ResultsOf 48 respondents with a median experience of 11.5 years in treating bvFTD, disinhibition was most frequently targeted (58.4%), followed by perseverative/compulsive behaviors (46.5%). Recommended drug classes included atypical antipsychotics (35.1%), selective serotonin reuptake inhibitors (31.2%), antiepileptics (10.0%), serotonin antagonist and reuptake inhibitors (8.4%), benzodiazepines (4.0%), and others (11.4%).ConclusionsOur survey revealed diverse pharmacological treatment practices for behavioral symptoms in bvFTD, reflecting the expected radical heterogeneity in pharmacological treatment strategies. Notwithstanding this, results from this explorative survey could further inform future research directions, and thus in turn potentially aid in establishing more consensus on effective pharmacological management of bvFTD, while the field awaits the development of highly anticipated disease‐modifying treatment(s).
Ablation of CCL17‐positive hippocampal neurons induces inflammation‐dependent epilepsy
Authors: Eberhard Judith , Henning Lukas , Fülle Lorenz , Knöpper Konrad , Böhringer Jana , Graelmann Frederike J. , Hänschke Lea , Kenzler Julia , Brosseron Frederic , Heneka Michael T. , Domingos Ana I. , Eyerich Stefanie , Lochner Matthias , Weighardt Heike , Bedner Peter , Steinhäuser Christian , Förster Irmgard 
Published: November 2024

AbstractObjectiveNeuronal cell death and neuroinflammation are characteristic features of epilepsy, but it remains unclear whether neuronal cell death as such is causative for the development of epileptic seizures. To test this hypothesis, we established a novel mouse line permitting inducible ablation of pyramidal neurons by inserting simian diphtheria toxin (DT) receptor (DTR) cDNA into the Ccl17 locus. The chemokine CCL17 is expressed in pyramidal CA1 neurons in adult mice controlling microglial quiescence.MethodsSeizure activity in CCL17‐DTR mice was analyzed by electroencephalographic recordings following treatment with DT for 3 consecutive days. Neuroinflammation and neuronal cell death were evaluated by (immuno)histochemistry. Pharmacological inhibition of TNFR1 signaling was achieved by treatment with XPro1595, a dominant‐negative inhibitor of soluble tumor necrosis factor.ResultsNeuronal cell death was detectable 7 days (d7) after the first DT injection in heterozygous CCL17‐DTR mice. Spontaneous epileptic seizures were observed in the vast majority of mice, often with an initial peak at d6–9, followed by a period of reduced activity and a gradual increase during the 1‐month observation period. Microglial reactivity was overt from d5 after DT administration not only in the CA1 region but also in the CA2/CA3 area, shortly followed by astrogliosis. Reactive microgliosis and astrogliosis persisted until d30 and, together with neuronal loss and stratum radiatum shrinkage, reflected important features of human hippocampal sclerosis. Granule cell dispersion was detectable only 3 months after DT treatment. Application of XPro1595 significantly reduced chronic seizure burden without affecting the development of hippocampal sclerosis.SignificanceIn conclusion, our data demonstrate that sterile pyramidal neuronal death is sufficient to cause epilepsy in the absence of other pathological processes. The CCL17‐DTR mouse line may thus be a valuable model for further mechanistic studies on epilepsy and assessment of antiseizure medication.
Socialization of adolescent emotion regulation through interparental conflict: Adolescent versus parent report
Authors: Colton Kassidy C. , Godleski Stephanie A. 
Published: November 2024

AbstractObjectiveGuided by the emotional security theory and tripartite model of children's emotion regulation (e.g., Davies & Cummings, 1994; Morris et al., 2007), this study examined the indirect influence of both adolescent and parent reported interparental conflict on the concurrent link between parent and adolescent emotion regulation.BackgroundThere is strong empirical evidence of a direct link between parent and offspring emotion regulation. However, very little is known about how interparental conflict might serve as a mechanism linking parent and adolescent emotion regulation.MethodThe sample consisted of 70 parents and their adolescent children (Mage = 14.59, SD = 1.44). Both parents and adolescents self‐reported on levels of interparental conflict and their own emotion regulation difficulties through Qualtrics software.ResultsFindings from a structural equation model indicated that parental emotion dysregulation was associated with higher levels of self‐ and adolescent‐reported interparental conflict. However, only adolescent‐reported interparental conflict was associated with adolescent emotion dysregulation, which further emerged as a significant indirect effect. Post hoc analyses indicated that conflict frequency may drive the parent–child emotion regulation link.ConclusionThese results highlight interparental conflict as a potential mechanism in the parent–child emotion regulation link and also emphasize the importance of considering children's reports of interparental conflict.ImplicationsInterparental conflict might be an important target for intervention efforts when trying to implement healthy emotion regulation development in adolescence.
Recurrent Extreme Climatic Events Are Driving Gorgonian Populations to Local Extinction: Low Adaptive Potential to Marine Heatwaves
Authors: Ramirez‐Calero Sandra , Gómez‐Gras Daniel , Barreiro Aldo , Bensoussan Nathaniel , Figuerola‐Ferrando Laura , Jou Marc , López‐Sanz Àngel , López‐Sendino Paula , Medrano Alba , Montero‐Serra Ignasi , Pagès‐Escolà Marta , Linares Cristina , Ledoux Jean‐Baptiste , Garrabou Joaquim 
Published: November 2024

ABSTRACTExtreme climatic events (ECEs), such as marine heatwaves (MHWs), are a major threat to biodiversity. Understanding the variability in ecological responses to recurrent ECEs within species and underlying drivers arise as a key issue owing to their implications for conservation and population recovery. Yet, our knowledge on such ecological responses is limited since it has been frequently gathered following “single‐event approaches” focused on one particular event. These approaches provide snapshots of ecological responses but fall short of capturing heterogeneity patterns that may occur among recurrent ECEs, questioning current predictions regarding biodiversity trends. Here, we adopt a “multiple events” perspective to characterize the effects of recurrent ECEs on the ecological responses in Paramuricea clavata, a Mediterranean temperate coral threatened by MHWs. Through a common‐garden experiment repeated three consecutive years with the same individuals from three populations, we assessed the respective roles of environmental (year effect), genetic (population effect), and phenotypic (population‐by‐environment interactions effect) components in the ecological response to recurrent heat stress. The environmental component (year) was the main driver underlying the responses of P. clavata colonies across experiments. To build on this result, we showed that: (i) the ecological responses were not related to population (genetic isolation) and individual (multilocus heterozygosity) genetic make‐up, (ii) while all the individuals were characterized by a high environmental sensitivity (genotype‐by‐environment interactions) likely driven by in situ summer thermal regime. We confront our experimental results to in situ monitoring of the same individuals conducted in 2022 following two MHWs (2018, 2022). This confirms that the targeted populations harbor limited adaptive and plastic capacities to on‐going recurrent ECEs and that P. clavata might face unavoidable population collapses in shallow Mediterranean waters. Overall, we underscore the need to consider the recurrence of ECEs to assess threats to biodiversity and to forecast its evolution.
Advances in Miscanthus × Giganteus Planting Techniques May Increase Carbon Uptake in the Establishment Year
Authors: Aslan‐Sungur (Rojda) Guler , Boersma Nic , Moore Caitlin E. , Heaton Emily , Bernacchi Carl J. , Vanloocke Andy 
Published: November 2024

ABSTRACTAgricultural lands hold significant potential for CO2 sequestration, particularly when utilizing biomass crops and agricultural residues. Among these, Miscanthus × giganteus (mxg) stands out due to its high productivity and carbon sequestration capabilities. Recognizing the importance of such biomass crops, the Intergovernmental Panel on Climate Change (IPCC) has identified Bioenergy with Carbon Capture and Storage (BECCS) as a crucial strategy for achieving net‐zero CO2 emissions by 2050. This study examines the carbon uptake potential of mxg during its establishment year at the Sustainable Advanced Bioeconomy Research (SABR) farm in Iowa, USA, where mxg was planted at a density exceeding previous studies. Using eddy covariance (EC) measurements, we quantified the net ecosystem carbon exchange (NEE), and derived gross primary productivity (GPP), and ecosystem respiration (Reco). Our findings reveal that SABR's mxg exhibited a significant carbon uptake of −621 g C m−2, a threefold increase compared to a similar EC site in the “corn‐belt” (University of Illinois Energy Research Farm; UIEF), which was established with lower planting density and pre‐commercial planting equipment. Favorable growing conditions and advanced planting technologies at SABR likely contributed to this high carbon uptake. Comparisons with other global EC studies indicated a strong correlation between higher planting densities and greater carbon uptake. These results suggest that increasing mxg planting density can enhance carbon uptake, but further studies are necessary to evaluate the impacts under varying environmental conditions and management practices. Additionally, economic analyses are essential to determine the viability of higher planting densities. Our study underscores the potential of optimized mxg management practices to contribute significantly to CO2 uptake and supports the development of BECCS as a viable climate change mitigation strategy.
Efficacy and safety of recombinant activated factor VII in Glanzmann thrombasthenia: A systematic literature review
Authors: Saultier Paul , Grino Michel , Falaise Céline , Voisin Sophie , Lavenu‐Bombled Cécile , Ibrahim‐Kosta Manal , Petit Audrey , Boutroux Hélène , Desprez Dominique , Fiore Mathieu , d'Oiron Roseline , Alessi Marie‐Christine 
Published: November 2024

AbstractBackgroundPlatelet transfusion is considered the standard treatment for preventing or controlling severe haemorrhage in Glanzmann thrombasthenia (GT). However, platelet transfusion can have detrimental effects, including the production of anti‐GPIIb/IIIa isoantibodies or anti‐HLA antibodies (Ab) and platelet transfusion refractoriness. Recombinant activated factor VII (rFVIIa) has been proposed as an alternative treatment to platelet transfusion.MethodsWe analyzed data from 77 case reports including 100 subjects to investigate the effectiveness and safety of rFVIIa in combination with platelets or antifibrinolytics for preventing or treating non‐surgical bleeds, as well as surgical and obstetrical procedures in GT.ResultsThe dosage of rFVIIa was consistent with previous recommendations (90 µg/kg per infusion). In subjects without Ab/refractoriness (n = 56), rFVIIa was effective in managing 93% of non‐surgical bleeds (n = 42), 91% of minor (n = 11) and 92% of major (n = 26) surgical procedures and 89% of obstetrical procedures (n = 9). In subjects with Ab/refractoriness (n = 44), rFVIIa was effective in managing 90% of non‐surgical bleeds (n = 39), and 75% of minor (n = 12) and 100% of major (n = 17) surgical procedures. The use of rFVIIa was safe, with 4 (2.7%) serious adverse events associated with rFVIIa.ConclusionAlthough the use of rFVIIa is currently restricted to subjects with Ab/refractoriness or when platelets are not available, our findings suggest expanding the indications for rFVIIa to encompass GT without Ab/refractoriness. Frontline use of rFVIIa may be proposed when clinically possible to mitigate the risks associated with platelet transfusion.
RNAi library screening reveals Gβ1, Casein Kinase 2 and ICAP‐1 as novel regulators of LFA‐1‐mediated T cell polarity and migration
Authors: Haap‐Hoff Antje , Freeley Michael , Dempsey Eugene , Dunican Dara , Bennett Emily , Triglia Denise , Skubis‐Zegadlo Joanna , Mitchell Davies Anthony , Kelleher Dermot , Long Aideen 
Published: November 2024

AbstractThe αLβ2 integrin LFA‐1 plays a key role in T‐cell adhesion to the endothelial vasculature and migration into both secondary lymphoid organs and peripheral tissues via interactions with its target protein ICAM‐1, but the pathways that regulate LFA‐1‐mediated T‐cell polarity and migration are not fully understood. In this study we screened two RNAi libraries targeting G protein‐coupled receptors (GPCR)/GPCR‐associated proteins and kinases in a HuT 78 T cell line model of LFA‐1‐stimulated T‐cell migration. Based on staining of the actin cytoskeleton, multiple parameters to measure cell morphology were used to assess the contribution of 1109 genes to LFA‐1‐mediated T‐cell polarity and migration. These RNAi screens identified a number of both novel and previously identified genes that either increased or decreased the polarity and migratory capacity of these cells. Following multiparametric analysis, hierarchical clustering and pathway analysis, three of these genes were characterized in further detail using primary human T cells, revealing novel roles for the heterotrimeric G protein subunit Gβ1 and Casein Kinase 2 in LFA‐1‐mediated T‐cell polarity and migration in vitro. Our studies also highlighted a new role for ICAP‐1, an adaptor protein previously described to be associated with β1 integrins, in β2 integrin LFA‐1‐directed migration in T cells. Knockdown of ICAP‐1 expression in primary T cells revealed a role in cell polarity, cell velocity and transmigration towards SDF‐1 for this adaptor protein. This study therefore uncovers new roles for GPCR/GPCR‐associated proteins and kinases in T‐cell migration and provides potential novel targets for modulation of the T‐cell immune response.
Deciphering Rice Genotypes Under Submergence Stress and Optimum Condition for Yield Stability Analysis
Authors: Pradhan Prajjwal , Das Bimal , Kumar Deepak , Phani Victor , Kundu Surajit 
Published: November 2024

ABSTRACTIn the flood‐prone area, the improved high‐yielding variety of rice declines its vegetative growth and suffers substantial yield losses due to water stagnation. To address the issue measurement, the present work implemented 45 rice genotypes in a randomized complete block design with three replicates over three consecutive years (2018–2021) across submergence (E1, E3, E5) and optimum environments (E2, E4, E6) to assess the adaptability of these genotypes and identify the most desirable type by various stability indices. A significant genotype × environment interaction (GEI) was found in the combined ANOVA of yield and its component traits. The combined analyses of yield component traits through the multi‐trait stability index (MTSI) and yield‐stability statistics (YSi) found the most promising genotypes G20 and G32, respectively. The mean grain yield advantage was found in G18 under submerged conditions and G25 across the environments. A highly significant correlation (p < 0.01) exists between the stress tolerance index and yield in both submergence (r = 0.96) and normal (r = 0.90) circumstances. Among genotypes G18, G5 and G19, G20 showed strong stability for grain yield based on univariate stability parameters (YSi, σi2, Wi2, S2d and bi). An AMMI1 biplot analysis indicated that genotypes G37, G45, G32, G31, G27, G28, G19, G17 and G7 exhibited stability for grain yields, with IPCA1 values approaching zero. The GGE biplot analysis on yield was constructed into two mega‐environments, where G18 (Narkel Chari), G31 (CR Dhan‐500), G9 (Bhasha Manik) and G40 (SS‐1) were winners of submergence stress and G25 (Narayan Kamini) was the winner of normal environments.
Particle size gradation design and performance enhancement of quartz cores for precision casting
Authors: Peng Yong‐Hui , Zhou Wen‐Tao , Chen Guang , Kou Bao‐Hong , Ouyang Jing 
Published: November 2024

AbstractThis study proposes an approach for the design of gradation plans via the establishment of mathematical models for particle gradation, enabling control over the particle gradation of different sizes of SiO2. Silica‐based ceramic cores were produced follow the above strategy for precision casting, and the impact of particle gradation and sintering regimes on the shrinkage rate and relevant mechanical properties of the silica‐based ceramic cores were investigated. The results suggest that an optimal gradation plan can effectively enhance the density of silica‐based ceramic cores, thereby influencing the shrinkage rate and mechanical properties at both room and elevated temperatures. The shrinkage rate of the silica‐based ceramic cores is influenced by the chosen gradation particle size range of the silica; the sintering regimes have notable effects on mechanical properties of the silica‐based ceramic cores. The performance data of the samples demonstrate the efficacy of applying the gradation plan to the B60 samples with an average particle size of 60 µm in the largest grade. After undergoing a final sintering temperature at 1160°C and holding for 5 h, the shrinkage ratio could be limited to 1.33%, the room temperature bending strength reached 15.0 MPa, and the high‐temperature bending strength could be enhanced to 37.6 MPa. These findings present a significant reference values for the production of single‐crystal hollow blades.