bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute Freeze-Dried Mango Consumption With a High-Fat Meal has Minimal Effects on Postprandial Metabolism, Inflammation and Antioxidant Enzymes
Authors: O’Hara Crystal , Ojo Babajide , Emerson Sam R , Simenson Ashley J , Peterson Sandra , Perkins-Veazie Penelope , Payton Mark E , Hermann Janice , Smith Brenda J , Lucas Edralin A 
Published: August 2019

Objective: Postprandial fluxes in oxidative stress, inflammation, glucose, and lipids, particularly after a high-fat meal (HFM), have been implicated in the development of cardiovascular disease (CVD). The aim of this study is to determine whether acute freeze-dried mango consumption modulates the postprandial response to an HFM. We hypothesized that the addition of mango, which is a rich source of many bioactive components, to an HFM would lower postprandial triglycerides, glucose, and inflammation, and increase antioxidant enzymes, compared to a standard HFM alone. Methods: In a randomized cross-over study, 24 healthy adult males (18-25 years old) consumed a typical American breakfast (670 kcal; 58% fat) with or without the freeze-dried mango pulp (50 g). Lipids, glucose, antioxidant enzymes, and inflammatory markers were assessed at baseline/fasting and 1, 2, and 4 hours after the HFM. Results: Addition of mango resulted in lower glucose (95.8 ± 4.4 mg/dL; P = .002) and higher high-density lipoprotein cholesterol (HDL-C; 58.4 ± 2.7 mg/dL; P = .01) 1 hour post-HFM compared to control (glucose: 104.8 ± 5.4 mg/dL; HDL-C: 55.2 ± 2.3 mg/dL), although no differences were observed in triglycerides ( P = .88 for interaction). No significant meal × time interactions were detected in markers of inflammation (C-reactive protein, P = .17; interleukin-6, P = .30) or antioxidant enzymes (superoxide dismutase, P = .77; glutathione peroxidase, P = .36; catalase, P = .32) in the postprandial period. Conclusions: When added to an HFM, acute mango consumption had modest beneficial effects on postprandial glucose and HDL-C responses, but did not alter triglyceride, inflammatory, or antioxidant enzymes.
Validation of the RUDAS in Patients With a Middle-Level Education in Lima, Peru
Authors: Custodio Nilton , Montesinos Rosa , Lira David , Herrera-Perez Eder , Chavez Kristy , Hernandez-Córdova Gustavo , Cuenca José , Gamboa Carlos , Metcalf Tatiana 
Published: August 2019

Objectives: To assess the psychometric properties of the Peruvian version of the Rowland Universal Dementia Assessment Scale (RUDAS-PE) to discriminate controls from patients with mild cognitive impairment (MCI) and early dementia (ED) in a population with a mid-level education. Methods: A total of 133 patients from a memory clinic were administered the RUDAS-PE, INECO Frontal Screening, Addenbrooke’s Cognitive Examination, and Mini-Mental State Examination. Results were compared against a neuropsychological evaluation (gold standard). Validity measures, internal consistency, and concurrent validity were calculated. Results: Cronbach’s α was 0.68; Pearson’s ratio was 0.79 ( P < .01). The area under the receiver–operating characteristics curve of the RUDAS to discriminate between ED and MCI was 89.0% (optimal cutoff at <21), whereas between MCI and controls, it was 99.0% (optimal cutoff at <24). Conclusions: The RUDAS-PE has acceptable psychometric properties performing well in its ability to discriminate controls from patients with MCI and ED.
Prognostic relevance of DNA damage and repair biomarkers in elderly patients with hormone-receptor-positive breast cancer treated with neoadjuvant hormone therapy: evidence from the real-world setting
Authors: Di Benedetto Anna , Ercolani Cristiana , Pizzuti Laura , Angelucci Domenico , Sergi Domenico , Marinelli Camilla , Iezzi Laura , Sperati Francesca , Terrenato Irene , Mazzotta Marco , Mariani Luciano , Vizza Enrico , Paoletti Giancarlo , Tomao Silverio , Maugeri-Saccà Marcello , Barba Maddalena , Tinari Nicola , Natoli Clara , Ciliberto Gennaro , Grassadonia Antonino , Vici Patrizia 
Published: August 2019

Background: The logic behind the outcome of endocrine therapy in breast cancer has long remained poorly understood. The prognostic role of DNA damage and repair biomarkers (DDR) was explored in postmenopausal, hormone-receptor-positive breast cancer patients treated with neoadjuvant hormone therapy (NAHT). Methods: Data on 55 patients were included. The phosphorylated ataxia-teleangectasia and Rad3-related protein (pATR), phosphorylated ataxia-telangiectasia mutated (ATM) kinase, and phosphorylated H2A Histone Family Member X (γ-H2AX) were evaluated by immunohistochemistry in paired tissues collected at baseline and following NAHT. Biomarkers were considered both singularly and within signatures. Ki-67 percentage change was the primary biomarker endpoint. Classical endpoints were also considered. Results: The most favorable Ki-67 outcome was associated with the γ-H2AX/pATM signature ( p = 0.011). In models of Ki-67 reduction, ‘luminal B’ subtype, higher grade of anaplasia, and the γ-H2AX/pATM signature tested as significant ( p < 0.05 for all). Results were confirmed in multivariate analysis. No association was observed with pathologic response. An increase of ∆γ-H2AX in paired breast tissues was associated with longer event-free survival ( p = 0.027) and overall survival ( p = 0.042). In Cox models, both survival outcomes were solely affected by grade of anaplasia, with less favorable prognosis in the highest grades ( p < 0.05 for both). Conclusions: We report novel evidence of the prognostic role of DDR biomarkers on important patient outcomes in postmenopausal hormone-receptor-positive breast cancer patients treated with NAHT. If confirmed in future and adequately sized trials, our results may help inform therapeutic decisions and clarify underlying biological mechanisms.
A narrative review evaluating the safety and efficacy of e-cigarettes as a newly marketed smoking cessation tool
Authors: Worku Dominic , Worku Elliott 
Published: August 2019

Introduction: E-cigarettes are an alternative to traditional tobacco-based cigarettes. While having considerable societal awareness, conflicting evidence exists to support their claims that they are an effective smoking cessation tool and are safe. Currently >7000 flavours exist with evidence that they exhibit detrimental cellular and tissue effects. A literature review was conducted utilising PubMed and Google Scholar Databases identifying papers between 2014 and 2019. The aims of this study were to accurately gauge the safety and efficacy of e-cigarettes as a smoking cessation tool. Methods: Search terms including ‘electronic cigarettes’ and ‘vaping’ were used to identify suitable references. A total of 314 articles were identified from which papers were excluded due to risk of bias, insufficient detail or were duplicate from which 58 papers were used in the final review. Results: Evidence shows that e-cigarettes can have detrimental effects on several cell lines and animal models with their flavourings and nicotine content implicated; this has, however, not translated into major health outcomes after 3.5 years follow-up but has been linked to chronic lung disease and cardiovascular disease. While advertised as an effective smoking cessation tool, no consensus can be made regarding their effectiveness although the first robust randomised controlled trial reports some success. This, however, is offset by the fact that the most common e-cigarette use is as a dual user and that there is evidence of threefold increased risk of future tobacco smoking. Conclusion: Future research is needed to evaluate the long-term health outcomes and efficacy of e-cigarettes as a smoking cessation tool with greater discussion between patients and clinicians regarding this smoking cessation tool.
Keloid research: current status and future directions
Authors: Tsai Chia-Hsuan , Ogawa Rei 
Published: August 2019

Introduction: Keloids and hypertrophic scars are fibroproliferative disorders of the skin that result from abnormal healing of injured or irritated skin. Multiple studies suggest that genetic, systemic and local factors may contribute to the development and/or growth of keloids and hypertrophic scars. A key local factor may be mechanical stimuli. Here, we provide an up-to-date review of the studies on the roles that genetic variation, epigenetic modifications and mechanotransduction play in keloidogenesis. Methods: An English literature review was performed by searching the PubMed, Embase and Web of Science databases with the following keywords: genome-wide association study; epigenetics; non-coding RNA; microRNA; long non-coding RNA (lncRNA); DNA methylation; mechanobiology; and keloid. The searches targeted the time period between the date of database inception and July 2018. Results: Genetic studies identified several single-nucleotide polymorphisms and gene linkages that may contribute to keloid pathogenesis. Epigenetic modifications caused by non-coding RNAs (e.g. microRNAs and lncRNAs) and DNA methylation may also play important roles by inducing the persistent activation of keloidal fibroblasts. Mechanical forces and the ensuing cellular mechanotransduction may also influence the degree of scar formation, scar contracture and the formation/progression of keloids and hypertrophic scars. Conclusions: Recent research indicates that the formation/growth of keloids and hypertrophic scars associate clearly with genetic, epigenetic, systemic and local risk factors, particularly skin tension around scars. Further research into scar-related genetics, epigenetics and mechanobiology may reveal molecular, cellular or tissue-level targets that could lead to the development of more effective prophylactic and therapeutic strategies for wounds/scars in the future.
RETRACTED ARTICLE: The specific MYB binding sites bound by TaMYB in the GAPCp2/3 promoters are involved in the drought stress response in wheat
Authors: Zhang Lin , Song Zhiqiang , Li Fangfang , Li Xixi , Ji Haikun , Yang Shushen 
Published: August 2019

Abstract
Background
Drought stress is one of the major abiotic stresses that affects plant growth and productivity. The GAPCp genes play important roles in drought stress tolerance in multiple species. The aim of this experiment was to identify the core cis-regulatory elements that may respond to drought stress in the GAPCp2 and GAPCp3 promoter sequences.

Results
In this study, the promoters of GAPCp2 and GAPCp3 were cloned. The promoter activities were significantly improved under abiotic stress via regulation of Rluc reporter gene expression, while promoter sequence analysis indicated that these fragments were not almost identical. In transgenic Arabidopsis with the expression of the GUS reporter gene under the control of one of these promoters, the activities of GUS were strong in almost all tissues except the seeds, and the activities were induced after abiotic stress. The yeast one-hybrid system and EMSA demonstrated that TaMYB bound TaGAPCp2P/3P. By analyzing different 5′ deletion mutants of these promoters, it was determined that TaGAPCp2P (− 1312~ − 528) and TaGAPCp3P (− 2049~ − 610), including the MYB binding site, contained enhancer elements that increased gene expression levels under drought stress. We used an effector and a reporter to co-transform tobacco and found that TaMYB interacted with the specific MYB binding sites of TaGAPCp2P (− 1197~ − 635) and TaGAPCp3P (− 1456~ − 1144 and − 718~ − 610) in plant cells. Then, the Y1H system and EMSA assay demonstrated that these MYB binding sites in TaGAPCp2P (− 1135 and − 985) and TaGAPCp3P (− 1414 and − 665) were the target cis-elements of TaMYB. The deletion of the specific MYB binding sites in the promoter fragments significantly restrained the drought response, and these results confirmed that these MYB binding sites (AACTAAA/C) play vital roles in improving the transcription levels under drought stress. The results of qRT-PCR in wheat protoplasts transiently overexpressing TaMYB indicated that the expression of TaGAPCp2/3 induced by abiotic stress was upregulated by TaMYB.

Conclusion
The MYB binding sites (AACTAAA/C) in TaGAPCp2P/3P were identified as the key cis-elements for responding to drought stress and were bound by the transcription factor TaMYB.
Commitment devices in the treatment of diabetic foot ulcers
Authors: Jarl Gustav 
Published: August 2019

AbstractBackgroundNon‐removable offloading devices are recommended for the treatment of uncomplicated plantar diabetic foot ulcers because adherence to using removable devices is low. However, patients may not always understand how crucial the non‐removability is to ulcer healing, leaving them with the impression that it is the device per se that heals the ulcer. Thus, after ulcer healing when patients return to using removable offloading devices, typically therapeutic footwear, they often return to a low level of adherence resulting in high reulceration rates. To change this pattern of behavior based on a misconception, we need to start with how we as clinicians are conceptualizing treatment with offloading devices.Non‐removable offloading devices as commitment devicesCommitment devices are voluntary restrictions people put on their future selves to resist short‐term temptations and achieve long‐term goals. In this paper, it is suggested that a change from viewing non‐removable offloading devices as means to force compliance, to viewing them as commitment devices could facilitate a change to a clinical thinking that emphasizes the importance of high adherence without compromising respect for patient autonomy.ConclusionViewing non‐removable offloading devices as commitment devices seems to be a promising approach to emphasize the importance of adherence while respecting patient autonomy. Hopefully, patients' higher appreciation of the role of adherence can lead to higher adherence to using therapeutic footwear after healing and consequently to reduced reulceration rates.
Crustal architecture of the northwestern and central Gulf of Mexico from integrated geophysical analysis
Authors: Filina Irina 
Published: August 2019

The tectonic history of the Gulf of Mexico (GOM) is a subject for ongoing debate. The nature of the crust in the northwestern and central parts of the basin remains poorly understood. Joined interpretation of two 2D seismic cross sections — GUMBO1 and GUMBO2 — with potential fields (gravity and magnetics) constrained with available well data allows testing various hypotheses about the subsurface structures and crustal architecture in the study area. In the northwestern GOM, two contradicting hypotheses about the nature of the crust were tested — exhumed mantle versus a thinned and intruded continental crust resulted from magma-rich rifting. The nature of the crust was also investigated in the central GOM, where the disagreement in the location of the ocean-continent boundary (OCB) from various published tectonic models reaches 140 km (87 mi). The results suggest that the crust in the study area is stretched continental with multiple magmatic additions represented by dense and highly magnetic bodies with fast seismic velocities, presumably introduced during the magma-assisted rifting of the GOM. The contact between oceanic and continental domains, i.e., the OCB, is interpreted to be near the Sigsbee Escarpment for both modeled lines. The analysis does not support the presence of thick presalt sediments in the study area. This result questions the currently accepted tectonic reconstructions of the GOM as thick presalt deposits are imaged confidently by various seismic surveys along the western Yucatan margin, which is believed to be a conjugate for the study area. This apparent mismatch in distribution of the presalt sediments requires further investigation.
Keratins regulate Hsp70-mediated nuclear localization of p38 mitogen-activated protein kinase
Authors: Lee So-Young , Kim Sujin , Lim Younglan , Yoon Han-Na , Ku Nam-On 
Published: August 2019

ABSTRACT
Intermediate filament protein keratin 8 (K8) binds to heat shock protein 70 (Hsp70) and p38 MAPK, and is phosphorylated at Ser74 by p38α (MAPK14, hereafter p38). However, a p38 binding site on K8 and the molecular mechanism of K8-p38 interaction related to Hsp70 are unknown. Here, we identify a p38 docking site on K8 (Arg148/149 and Leu159/161) that is highly conserved in other intermediate filaments. A docking-deficient K8 mutation caused increased p38-Hsp70 interaction and enhanced p38 nuclear localization, indicating that the p38 dissociated from mutant K8 makes a complex with Hsp70, which is known as a potential chaperone for p38 nuclear translocation. Comparison of p38 MAPK binding with keratin variants associated with liver disease showed that the K18 I150V variant dramatically reduced binding with p38, which is similar to the effect of the p38 docking-deficient mutation on K8. Because the p38 docking site on K8 (Arg148/149 and Leu159/161) and the K18 Ile150 residue are closely localized in the parallel K8/K18 heterodimer, the K18 I150V mutation might interfere with K8-p38 interaction. These findings show that keratins, functioning as cytoplasmic anchors for p38, modulate p38 nuclear localization and thereby might affect a number of p38-mediated signal transduction pathways.
Unsupervised Isometric Exercise versus Wait-and-See for Lateral Elbow Tendinopathy
Authors: VUVAN VIANA , VICENZINO BILL , MELLOR REBECCA , HEALES LUKE JAMES , COOMBES BROOKE KAYE 
Published: August 2019

ABSTRACT

Purpose
This study aimed to investigate the effect of unsupervised isometric exercise compared with a wait-and-see approach on pain, disability, global improvement, and pain-free grip strength in individuals with lateral elbow tendinopathy.

Methods
Forty participants with unilateral lateral elbow tendinopathy of at least 6 wk duration were randomized to either wait-and-see (n = 19) or a single supervised instruction session by a physiotherapist, followed by an 8-wk unsupervised daily program of progressive isometric exercise (n = 21). Primary outcomes were Patient-Rated Tennis Elbow Evaluation, global rating of change on a six-point scale (dichotomized to success and no success) and pain-free grip strength at 8 wk. Secondary outcomes were resting and worst pain on an 11-point numerical rating scale, and thermal and pressure pain thresholds as a measure of pain sensitivity.

Results
Thirty-nine (98%) participants completed 8-wk measurements. The exercise group had lower Patient-Rated Tennis Elbow Evaluation scores compared with wait-and-see at 8 wk (standardized mean difference [SMD], −0.92; 95% confidence interval [CI], −1.58 to −0.26). No group differences were found for success on global rating of change (29% exercise vs 26% wait-and-see (risk difference, 2.3%; 95% CI, −24.5 to 29.1)), or pain-free grip strength (SMD, −0.33; 95% CI, −0.97 to 0.30). No differences were observed for all secondary outcomes except for worst pain, which was moderately lower in the exercise group (SMD, −0.80; 95% CI, −1.45 to −0.14).

Conclusions
Unsupervised isometric exercise was effective in improving pain and disability, but not perceived rating of change and pain-free grip strength when compared with wait-and-see at 8 wk. With only one of the three primary outcomes being significantly different after isometric exercises, it is doubtful if this form of exercise is efficacious as a sole treatment.
Does Eccentric-only and Concentric-only Activation Increase Power Output?
Authors: KRZYSZTOFIK MICHAL , WILK MICHAL , GOLAS ARTUR , LOCKIE ROBERT GEORGE , MASZCZYK ADAM , ZAJAC ADAM 
Published: August 2019

ABSTRACT

Purpose
The aim of study was to evaluate changes in power output and bar velocity in the bench press throw (BPT) following the bench press (BP) exercise as a conditioning activity (CA) with concentric only (CONONLY) and eccentric only (ECCONLY) contractions.

Methods
Thirty-two (n = 32) healthy strength-trained men participated in this study (age, 28.4 ± 4.5 yr; height, 177 ± 7.6 cm; body mass, 93.5 ± 9.3 kg; BP one-repetition maximum (1RM), 143.6 ± 17.5 kg). The experiment was performed following a randomized crossover design, where each participant performed 2 sets of 2 repetitions using the BP exercise as the CA at 90% 1RM ECCONLY, 90% 1RM CONONLY, 110% 1RM ECCONLY, or 130% 1RM ECCONLY contraction. The BPT was performed to assess changes in peak power (PP), mean power (MP), and peak velocity (PV), mean velocity (MV) before and after CA. The differences between analyzed variables before and after the CA were verified using ANOVA with repeated measures. Statistical significance was set at P < 0.05.

Results
There were statistically significant differences between baseline and postactivation value of PP and PV in the BPT (P < 0.05) after the CA with ECCONLY contraction at 110% 1RM and at 130% 1RM as well between baseline and postactivation value of MV in the BPT (P < 0.05) after CA with contraction at 110% 1RM. There were no significant differences between baseline and postactivation values of PP, MP, PV, and MV in the BPT after the CA with CONONLY contraction at 90% 1RM and CA with ECCONLY contraction at 90% 1RM.

Conclusions
Partial movement with ECCONLY contraction is effective in short-term power output development, but only when the load used in the CA exceeds 100% 1RM.
Case Report: Spontaneous perforation of choledochal cyst in an infant: Successful management in a centre with limited means
Authors: Rahman Rafey Abdul , Gupta Umesh Kumar 
Published: August 2019

Background: Biliary peritonitis due to a ruptured choledochal cyst (CC) is a rare occurrence. The difference between bile duct perforation (BDP) and ruptured choledochal cysts continues to be a matter of debate. Simple drainage, T tube placement and cholecystostomy have been proposed as the initial treatment of choice. Definitive surgery in the form excision of the CC and hepatico-enterostomy has been described as the ideal treatment option. We report a successful management of a unique case of perforated choledochal cyst in an infant who presented with biliary peritonitis. Case report: An 8 months old female child presented with biliary peritonitis as result of spontaneous perforation of a choledochal cyst. The patient was successfully managed initially by placement of T tube in the perforated cyst followed by a T tube cholangiogram. Definitive surgery was performed 5 weeks after the initial surgery in which cyst was excised and hepatico-duodenostomy was performed. The child is currently in follow up and doing well. Conclusion: Perforated CC can present as acute abdomen sometimes having only subtle signs. In absence of any previous established diagnosis of CC and trained radiological support the condition becomes challenging to diagnose preoperatively. External T-tube drainage followed by T-tube cholangiogram can help in delineating the anatomy. Cyst excision along with hepaticoportoentersomy remains the gold standard definitive treatment.