bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spreading depolarizations trigger caveolin‐1–dependent endothelial transcytosis
Authors: Sadeghian Homa , Lacoste Baptiste , Qin Tao , Toussay Xavier , Rosa Roberto , Oka Fumiaki , Chung David Y. , Takizawa Tsubasa , Gu Chenghua , Ayata Cenk 
Published: July 2018

ObjectiveCortical spreading depolarizations (CSDs) are intense and ubiquitous depolarization waves relevant for the pathophysiology of migraine and brain injury. CSDs disrupt the blood–brain barrier (BBB), but the mechanisms are unknown.MethodsA total of six CSDs were evoked over 1 hour by topical application of 300 mM of KCl or optogenetically with 470 nm (blue) LED over the right hemisphere in anesthetized mice (C57BL/6 J wild type, Thy1‐ChR2‐YFP line 18, and cav‐1–/–). BBB disruption was assessed by Evans blue (2% EB, 3 ml/kg, intra‐arterial) or dextran (200 mg/kg, fluorescein, 70,000 MW, intra‐arterial) extravasation in parietotemporal cortex at 3 to 24 hours after CSD. Endothelial cell ultrastructure was examined using transmission electron microscopy 0 to 24 hours after the same CSD protocol in order to assess vesicular trafficking, endothelial tight junctions, and pericyte integrity. Mice were treated with vehicle, isoform nonselective rho‐associated kinase (ROCK) inhibitor fasudil (10 mg/kg, intraperitoneally 30 minutes before CSD), or ROCK‐2 selective inhibitor KD025 (200 mg/kg, per oral twice‐daily for 5 doses before CSD).ResultsWe show that CSD‐induced BBB opening to water and large molecules is mediated by increased endothelial transcytosis starting between 3 and 6 hours and lasting approximately 24 hours. Endothelial tight junctions, pericytes, and basement membrane remain preserved after CSDs. Moreover, we show that CSD‐induced BBB disruption is exclusively caveolin‐1–dependent and requires rho‐kinase 2 activity. Importantly, hyperoxia failed to prevent CSD‐induced BBB breakdown, suggesting that the latter is independent of tissue hypoxia.InterpretationOur data elucidate the mechanisms by which CSDs lead to transient BBB disruption, with diagnostic and therapeutic implications for migraine and brain injury.
Induction chemotherapy in the treatment of nasopharyngeal carcinoma: Clinical outcomes and patterns of care
Authors: Gabani Prashant , Barnes Justin , Lin Alexander J. , Rudra Soumon , Oppelt Peter , Adkins Douglas , Rich Jason T. , Zevallos Jose P. , Daly Mackenzie D. , Gay Hiram A. , Thorstad Wade L. 
Published: July 2018

AbstractThe role of induction chemotherapy in nasopharyngeal carcinoma (NPC) remains controversial. The primary aim of this study was to use the National Cancer Database to evaluate the patterns of care of induction chemotherapy in NPC and its impact on overall survival (OS). Patients with NPC from 2004 to 2014 were obtained from the NCDB. Patients were considered to have received induction chemotherapy if it was started ≥43 days before the start of RT and concurrent CRT if chemotherapy started within 21 days after the start of RT. Propensity score matching was used to control for selection bias. Cox proportional hazards model was used to determine significant predictors of OS. Logistic regression model was used to determine predictors of the use of induction chemotherapy. Significance was defined as a P value <.05. A total of 4857 patients were identified: 4041 patients (87.2%) received concurrent CRT and 816 patients (16.8%) received induction chemotherapy. The use of induction therapy remained stable between 2004 and 2014. Younger patients and those with higher T‐ and N‐stage had a higher likelihood of being treated with induction chemotherapy. The 5‐year OS in patients treated with induction chemotherapy and CRT was 66.3% vs 69.1%, respectively (P = .25). There was no difference in OS when these two groups were analyzed after propensity score matching. No differences in OS existed between these treatment groups in patients with T3‐T4N1 or TanyN2‐3 disease (P = .76). Propensity score matching also did not reveal any difference in OS in patients with T3‐T4N1 or TanyN2‐3 disease. The use of induction chemotherapy has remained stable in the last decade. In this study of patients with NPC, induction chemotherapy was not associated with improved OS compared to CRT alone.
Conservation of energy, density of states, and spin lattice relaxation
Authors: Khaneja Navin 
Published: July 2018

AbstractThe starting point of all NMR experiments is a spin polarization which develops when we place the sample in static magnetic field B0. There are excess of spins aligned along B0 (spin up with lower energy) than spins aligned opposite (spin down with higher energy) to the field B0. A natural question is what is the source of this excess spin polarization because relaxation mechanisms can flip a up spin to a down spin and vice‐versa. The answer lies in the density of states. When a molecule with spin down flips to spin up it loses energy. This energy goes into increasing the kinetic energy of the molecule in the gas/solution phase. At this increased kinetic energy, there are more rotational‐translational states accessible to the molecule than at lower energy. This increases the probability the molecule will spend in spin up state (higher kinetic energy state). This is the source of excess polarization. In this article, we use an argument based on equipartition of energy to explicitly count the excess states that become accessible to the molecule when its spin is flipped from down to up. Using this counting, we derive the familiar Boltzmann distribution of the ratio of up vs down spins. Although prima facie, there is nothing new in this article, we find the mode counting argument for excess states interesting. Furthermore, the article stresses the fact that spin polarization arises from higher density of states at increased kinetic energy of molecules.
Systemic inflammation in acute cardiorenal syndrome: an observational pilot study
Authors: Linhart Christoph , Ulrich Christof , Greinert Daniel , Dambeck Stefanie , Wienke Andreas , Girndt Matthias , Pliquett Rainer U. 
Published: July 2018

AbstractAimsAcute cardiorenal syndrome (CRS) with and without consideration of the volume state was assessed with regard to inflammatory parameters.Methods and resultsBlood samples from patients with acute CRS (Ronco type 1 or 3, Group 1, n = 15), end‐stage renal disease (Group 2, n = 12), hypertension (Group 3, n = 15), and, in a second cohort, with acute CRS and hypervolemia (Group 4, n = 9) and hypertension (Group 5, n = 10) were analysed with regard to lipopolysaccharide‐binding protein (LBP), interleukins (ILs), and monocyte function (flow cytometry) both on admission (all groups) and on discharge (Groups 1 and 4). By discharge, one Group 1 patient died. LBP (ANOVA for Groups 1–3: P = 0.001) and IL‐6 (Kruskal–Wallis for Groups 1–3: P < 0.0001) were higher in Group 1 (LBP: 11.7 ± 2.0 μg/mL; IL‐6: 15.0 ± 6.1 pg/mL) and in Group 2 (LBP: 10.4 ± 1.4 μg/mL; IL‐6: 14.6 ± 3.8 pg/mL) than in Group 3 (LBP: 5.8 ± 0.4 μg/mL; IL‐6: 1.8 ± 0.4 pg/mL). In a direct comparison, the proportion of activated monocytes (CD14 and CD16 positive) was higher in Group 1 (6.9% ± 0.7%) vs. Group 3 (5.1% ± 0.6%; P = 0.018). Group 4 patients had higher IL‐6 plasma levels (34.2 ± 10.1 pg/mL) than Group 1 patients (15.0 ± 6.1 pg/mL; P = 0.03). All other findings obtained in CRS groups (Groups 1 and 4) were comparable.ConclusionsIn acute CRS, a state of systemic inflammation was found, which is comparable with the end‐stage renal disease situation. In comparison with hypertensive controls, a monocytic activation was found in acute CRS regardless of volume state.
Natural extracts from fresh and oven‐dried winemaking by‐products as valuable source of antioxidant compounds
Authors: Marchante Lourdes , Gómez Alonso Sergio , Alañón María Elena , Pérez‐Coello María Soledad , Díaz‐Maroto María Consuelo 
Published: July 2018

AbstractWinemaking by‐products are a natural source of antioxidant components; however, due to their highly perishable and seasonal nature, they may require a prior conservation step before being processed. Natural extracts from fresh and oven‐dried red grape agro‐industrial by‐products were obtained by ultrasound assisted extraction (UAE), using a hydroalcoholic solution as extracting solvent. Extracts were analyzed by HPLC‐DAD‐ESI‐MS, to know the feasibility of winemaking by‐products as natural sources of phenolic compounds, as well as the effect of the oven‐drying treatment on the phenolic composition. Oven‐drying at 45°C caused a significant decrease on the total phenolic content, which implied a reduction of the antioxidant capacity of the extracts. Also, it produced a decrease in total and individual flavan‐3‐ols, stilbenes, and flavonols, being greater in those extracts from stems. Respect to anthocyanins, which were only identified in grape pomace extracts, oven‐drying caused an important decrease, being the peonidin‐3‐O‐glucoside the more thermosensitive compound. Natural extracts from fresh or oven‐dried winemaking by‐products could be used in other food industries as a valuable source of phenolic compounds with antioxidant properties. However, further studies on other drying methods are required for addressing the preservation of phenolic compounds from winery by‐products successfully.
Entrepreneurship Assessment in Higher Education: A Research Review for Engineering Education Researchers
Authors: Huang‐Saad Aileen Y. , Morton Christina S. , Libarkin Julie C. 
Published: July 2018

AbstractBackgroundDespite the wide adoption of entrepreneurship by United States engineering programs, there have been few advances in how to measure the influences of entrepreneurial education on engineering students. We believe the inadequate growth in engineering entrepreneurship assessment research is due to the limited use of research emerging from the broader entrepreneurship education assessment community.PurposeThis paper explores entrepreneurship education assessment by documenting the current state of the research and identifying the theories, variables, and research designs most commonly used by the broader community. We then examine if and how these theories and constructs are used in engineering entrepreneurship education.Scope/MethodTwo literature databases, Scopus® and Proquest, were searched systematically for entrepreneurship education assessment research literature. This search yielded 2,841 unique papers. Once inclusion and exclusion criteria were applied, 359 empirical research papers were coded for study design, theory, variables measured, instruments, and validity and reliability.ConclusionsWhile there has been growth in entrepreneurship education assessment research, little exchange of ideas across the disciplines of business, engineering, and education is occurring. Nonempirical descriptions of programs outweigh empirical research, and these empirical studies focus on affective, rather than cognitive or behavioral, outcomes. This pattern within the larger entrepreneurship community is mirrored in engineering where the use of theory‐based, validated entrepreneurship education assessment instruments generally focuses on the context of intent to start a new company. Given the engineering community's goals to support engineering entrepreneurship beyond business creation, the engineering education community should consider developing assessment instruments based in theory and focused on engineering‐specific entrepreneurship outcomes.
Inoculation of tomato plants with selected PGPR represents a feasible alternative to chemical fertilization under salt stress
Authors: Cordero Irene , Balaguer Luis , Rincón Ana , Pueyo José J. 
Published: July 2018

AbstractPlant growth‐promoting rhizobacteria (PGPR) are soil bacteria that colonize the rhizosphere of plants, enhance plant growth, and may alleviate environmental stress, thus constituting a powerful tool in sustainable agriculture. Here, we compared the capacity of chemical fertilization to selected PGPR strains to promote growth and alleviate salinity stress in tomato plants (Solanum lycopersicum L.). A pot experiment was designed with two main factors: fertilization (chemical fertilization, bacterial inoculation with seven PGPR, or a non‐fertilized non‐inoculated control) and salt stress (0 or 100 mM NaCl). In the absence of stress, a clear promotion of growth, a positive effect on plant physiology (elevated Fv/Fm), and enhanced N, P, and K concentrations were observed in inoculated plants compared to non‐fertilized controls. Salinity negatively affected most variables analyzed, but inoculation with certain strains reduced some of the negative effects on growth parameters and plant physiology (water loss and K+ depletion) in a moderate but significant manner. Chemical fertilization clearly exceeded the positive effects of inoculation under non‐stressed conditions, but conversely, biofertilization with some strains outperformed chemical fertilization under salt stress. The results point at inoculation with selected PGPR as a viable economical and environment‐friendly alternative to chemical fertilization in salinity‐affected soils.
Effect of activity states on habitat selection by black‐tailed deer
Authors: Bose Samhita , Forrester Tavis D. , Casady David S. , Wittmer Heiko U. 
Published: July 2018

ABSTRACTHabitat selection is a complex hierarchical process and in ungulates typically varies at broad spatial and temporal scales and among individuals. Recent advancements in the ability of global positioning system (GPS)‐collars to collect activity data provide opportunities to understand underlying mechanisms or trade‐offs responsible for fine‐scale variation in habitat selection. Based on data from 64 female black‐tailed deer (Odocoileus hemionus columbianus) fitted with GPS‐collars in northern California, USA, between 2004 and 2013, we first investigated habitat selection at the home range scale. We then used data from a subset of 27 individuals to determine if habitat selection within home ranges was influenced by activity states (active or inactive). Habitat selection by black‐tailed deer varied between summer and winter and was mostly explained by differences in elevation, terrain, and vegetation. Within their home ranges, black‐tailed deer showed fine‐scale selection for habitats that varied with activity states. In summer, selection for edge density and forest types varied across activity states highlighting important fine‐scale selection patterns. Activity state also affected our conclusions about the selection of habitats including slope, canopy cover, and forest types by black‐tailed deer in winter. During both seasons, deer selected for apparently secure habitat when inactive, likely to minimize risk of predation. These results highlight the importance of considering activity states when evaluating habitat selection from animal location data. This is particularly important in multi‐use landscapes such as national forests where habitat needs of ungulates are important considerations in management decisions, including timber harvest. © 2018 The Wildlife Society.
American woodcock singing‐ground survey sampling of forest type and age
Authors: Tavernia Brian G. , Nelson Mark D. , Rau Rebecca , Garner James D. , Perry Charles H. 
Published: July 2018

ABSTRACTThe American Woodcock Conservation Plan calls for halting and reversing declines of American woodcock (Scolopax minor) populations through creation and management of early successional forest (ESF). Counts of displaying male woodcock along routes of the American woodcock singing‐ground survey (SGS) are used to assess regional population status and trends, and there is a need to assess whether SGS routes represent the region. We assessed whether individual SGS routes (330‐m buffers) in the Boreal‐Hardwood and Prairie‐Hardwood Transitions of Minnesota, USA represented land covers within local landscapes, defined using simulated 10‐minute blocks, and whether the routes, in aggregate, represented land covers of our study region. Our land covers included non‐forest classes, age‐based ESF (≤20 years), and persisting classes for deciduous‐mixed and evergreen forests and woody wetlands. We found that the median value of mean absolute differences (MAD) between percentages for route buffer and block cover classes was 3.78 percentage points. Twenty‐two of 81 (27%) route buffers had MAD values ≥5 percentage points. Within Minnesota, more of these routes (19 of 22) occurred in the Boreal‐Hardwood Transition than in the Prairie‐Hardwood Transition. Relative to local landscapes, route buffers most frequently and strongly under‐represented open water, barren land, evergreen ESF, persisting woody wetlands, and woody wetland ESF and over‐represented developed land and grassland‐pasture. When we compared routes in aggregate to our study region, the magnitude of percentage point differences for individual covers did not exceed 5, except for open water. Given the relatively small differences we observed, we conclude that SGS routes well represent land covers within our study region. © 2018 The Wildlife Society.
Exome sequencing of 85 Williams–Beuren syndrome cases rules out coding variation as a major contributor to remaining variance in social behavior
Authors: Kopp Nathan D. , Parrish Phoebe C. R. , Lugo Michael , Dougherty Joseph D. , Kozel Beth A. 
Published: July 2018

AbstractBackgroundLarge, multigenic deletions at chromosome 7q11.23 result in a highly penetrant constellation of physical and behavioral symptoms known as Williams–Beuren syndrome (WS). Of particular interest is the unusual social‐cognitive profile evidenced by deficits in social cognition and communication reminiscent of autism spectrum disorders (ASD) that are juxtaposed with normal or even relatively enhanced social motivation. Interestingly, duplications in the same region also result in ASD‐like phenotypes as well as social phobias. Thus, the region clearly regulates human social motivation and behavior, yet the relevant gene(s) have not been definitively identified.MethodHere, we deeply phenotyped 85 individuals with WS and used exome sequencing to analyze common and rare variation for association with the remaining variance in social behavior as assessed by the Social Responsiveness Scale.ResultsWe replicated the previously reported unusual juxtaposition of behavioral symptoms in this new patient collection, but we did not find any new alleles of large effect in the targeted analysis of the remaining copy of genes in the Williams syndrome critical region. However, we report on two nominally significant SNPs in two genes that have been implicated in the cognitive and social phenotypes of Williams syndrome, BAZ1B and GTF2IRD1. Secondary discovery driven explorations focusing on known ASD genes and an exome wide scan do not highlight any variants of a large effect.ConclusionsWhole exome sequencing of 85 individuals with WS did not support the hypothesis that there are variants of large effect within the remaining Williams syndrome critical region that contribute to the social phenotype. This deeply phenotyped and genotyped patient cohort with a defined mutation provides the opportunity for similar analyses focusing on noncoding variation and/or other phenotypic domains.
Association of hemoglobin with arterial stiffness evaluated by carotid‐femoral pulse wave velocity among Chinese adults
Authors: Zhang Zhen‐Zhen , Wang Ping , Kong Xiang‐Lei , Mao Wen‐Li , Cui Mei‐Yu 
Published: July 2018

AbstractObjectiveIncreased hemoglobin (Hb) levels are known to be associated with increased cardiovascular events and mortalities. Therefore, we assumed that high Hb levels were associated with arterial stiffness. Pulse wave velocity (PWV) is a simple and noninvasive method for measuring arterial stiffness to assess cardiovascular disease in general populations. Accordingly, we conducted a cross‐sectional study to explore the association of Hb with PWV.MethodsA total of 6642 adults aged 54.5 ± 11.2 years undergoing physical examinations were enrolled, 71.7% of whom were males. Arterial stiffness was evaluated by carotid‐femoral PWV (cfPWV). Multivariable regression analyses were performed to determine the relationship between Hb and increased cfPWV.ResultsIn this study, the mean Hb (per 10 g/L increase) was 144.7 ± 13.9 g/L, and the mean cfPWV was 15.1 ± 3.1 m/s. cfPWV was significantly higher in high hemoglobin groups ≥15.4 g/L (Quartile 4) than in the lowest hemoglobin group (Quartile 1 ≤ 13.6 g/L; P < 0.001). Multiple linear regression analysis revealed that Hb positively correlated with cfPWV (β = 0.16, P < 0.01). Univariate Logistic regression analysis revealed that Hb was associated with increased cfPWV, with an odd ratio (OR) of 1.46 (95% confidence interval [CI], 1.39–1.54). After adjusting for potential confounders, Hb and the highest Hb quartile group were also independently associated with increased cfPWV, with a fully adjusted OR of 1.11 (95% CI, 1.02–1.20) and 1.45 (95% CI, 1.01–2.08), respectively.ConclusionThis study demonstrated that Hb levels significantly correlate with increased cfPWV.
Inflatable Penile Prostheses Implantation: Does Antibiotic Exposure Matter?
Authors: Chanyi Ryan M. , Alzubaidi Raidh , Leung Everett J.Y. , Wilcox Hannah B. , Brock Gerald B. , Burton Jeremy P. 
Published: July 2018

Abstract

Background
Inflatable penile prosthetic (IPP) infections are unusual but carry high patient morbidity and healthcare costs.

Aim
To increase the bactericidal effect of IPP tubing material to prevent future bacterial infections and to determine whether this effect is time-dependent.

Methods
A modified disk diffusion assay was developed to measure the zones of inhibition against Escherichia coli, Proteus mirabilis, Staphylococcus aureus, and Staphylococcus epidermidis when tubing was immersed in gentamycin, ampicillin, tetracycline, kanamycin, erythromycin, or ciprofloxacin. To further assess the efficacy of this approach, IPP tubing was exposed to ampicillin or ciprofloxacin for 30 seconds, 2 minutes, 10 minutes, or 60 minutes.

Outcomes
Bacterial zones of inhibition against IPP tubing material exposed to various treatments.

Results
IPP tubing was more effective against Gram-positive bacteria (S aureus and S epidermidis) then Gram-negative bacteria (E coli and P mirabilis). Immersing IPP tubing material in ampicillin or ciprofloxacin increased bactericidal effect of tubing material against Gram-positive and Gram-negative bacteria, respectively. The observed inhibitory effect was time dependent.

Clinical Translation
Exposing IPP to a specific antimicrobial directly before implantation increases the bactericidal properties of the material, potentially decreasing the likelihood of infection.

Strengths & Limitations
This study is limited in that it is in vitro experimentation observing the effect of a single strain of each bacterium. Although the strains used were clinically relevant, further analysis is required to determine whether these results were strain specific.

Conclusion
Immersing IPP material into an antibiotic solution, such as ampicillin or ciprofloxacin, increases the bactericidal properties and may aid in the prevention of infection.