bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can we safely predict solvation Gibbs energies of pure and mixed solutes with a cubic equation of state?
Authors: Moine Edouard , Privat Romain , Jaubert Jean-Noël , Sirjean Baptiste , Novak Nefeli , Voutsas Epaminondas , Boukouvalas Christos 
Published: February 2019

Abstract
Solvation Gibbs energies are basically defined as a chemical potential change when transferring a fixed molecule from a perfect gas to a real liquid mixture. This quantity is of special interest for many practical applications as it quantifies the degree of affinity of a solute for its solvent. Few methods are currently available in the literature for the prediction of solvation Gibbs energies. In this article, a new approach is proposed: the use of a predictive cubic equation of state (EoS). The UMR-PRU (Universal Mixing Rule Peng-Robinson UNIFAC) EoS has been selected for its known capacity to semi-predict behaviors of complex systems including polar and associating compounds (by semi-prediction, it is meant that the EoS predicts binary interaction parameters but requires pure-component properties as input parameters). UMR-PRU predictions have been compared to experimental data extracted from the extensive CompSol database (containing around 22 000 pure component data and 70 000 binary data). Accurate predictions were obtained (a mean absolute deviation of 0.36 kcal/mol was obtained for all the binary data). Finally, when using a fully-predictive approach (i.e. pure-component EoS parameters are predicted from group-contribution methods), the prediction accuracy is roughly preserved.
Meta-analytic framework for modeling genetic coexpression dynamics
Authors: Kinzy Tyler G. , Starr Timothy K. , Tseng George C. , Ho Yen-Yi 
Published: February 2019

Abstract
Methods for exploring genetic interactions have been developed in an attempt to move beyond single gene analyses. Because biological molecules frequently participate in different processes under various cellular conditions, investigating the changes in gene coexpression patterns under various biological conditions could reveal important regulatory mechanisms. One of the methods for capturing gene coexpression dynamics, named liquid association (LA), quantifies the relationship where the coexpression between two genes is modulated by a third “coordinator” gene. This LA measure offers a natural framework for studying gene coexpression changes and has been applied increasingly to study regulatory networks among genes. With a wealth of publicly available gene expression data, there is a need to develop a meta-analytic framework for LA analysis. In this paper, we incorporated mixed effects when modeling correlation to account for between-studies heterogeneity. For statistical inference about LA, we developed a Markov chain Monte Carlo (MCMC) estimation procedure through a Bayesian hierarchical framework. We evaluated the proposed methods in a set of simulations and illustrated their use in two collections of experimental data sets. The first data set combined 10 pancreatic ductal adenocarcinoma gene expression studies to determine the role of possible coordinator gene USP9X in the Hippo pathway. The second experimental data set consisted of 907 gene expression microarray Escherichia coli experiments from multiple studies publicly available through the Many Microbe Microarray Database website (http://m3d.bu.edu/) and examined genes that coexpress with serA in the presence of coordinator gene Lrp.
Über das Sichtbare hinaus. Eine Soziologie künstlerischer Praxis
Authors: Schürkmann Christiane 
Published: February 2019

ZusammenfassungMit einer ethnografischen Perspektive untersucht der Aufsatz künstlerische Praxis im Feld der bildenden Kunst. Ins Zentrum rückt die Frage, wie Kunstwerke beziehungsweise künstlerische Arbeitenin situhervorgebracht werden. Dabei folgt die Ethnografin den PraktikerInnen respektive den KünstlerInnen und ihrem situierten Tun. In Form soziologisch-ethnografischer Analysen wird gezeigt, 1) wie eine bestimmte Praxis künstlerisch qualifizierten Sehens an Kunsthochschulen geübt wird, 2) wie künstlerische Praxis als Interaktion zwischen Körper und Material hervortritt, 3) wie KünstlerInnen ihre Arbeiten durch ästhetisches Antizipieren entwickeln und beurteilen und schließlich 4) wie KünstlerInnen sich gegenüber ausgestellten Werken kritisch positionieren. Formuliert wird der Ansatz einer Soziologie künstlerischer Praxis mit dem Ziel, Kunst in ihren epistemischen und sinnlichen Potenzialen für die Kunst- und Wissenssoziologie weitergehend zugänglich zu machen.
Increased Alcohol-Drinking Induced by Manipulations of mGlu5 Phosphorylation within the Bed Nucleus of the Stria Terminalis
Authors: Campbell Rianne R. , Domingo Racquel D. , Williams Amy R. , Wroten Melissa G. , McGregor Hadley A. , Waltermire Ryan S. , Greentree Daniel I. , Goulding Scott P. , Thompson Andrew B. , Lee Kaziya M. , Quadir Sema G. , Jimenez Chavez C. Leonardo , Coelho Michal A. , Gould Adam T. , von Jonquieres Georg , Klugmann Matthias , Worley Paul F. , Kippin Tod E. , Szumlinski Karen K. 
Published: February 2019

The bed nucleus of the stria terminalis (BNST) is part of the limbic-hypothalamic system important for behavioral responses to stress, and glutamate transmission within this region has been implicated in the neurobiology of alcoholism. Herein, we used a combination of immunoblotting, neuropharmacological and transgenic procedures to investigate the role for metabotropic glutamate receptor 5 (mGlu5) signaling within the BNST in excessive drinking. We discovered that mGlu5 signaling in the BNST is linked to excessive alcohol consumption in a manner distinct from behavioral or neuropharmacological endophenotypes that have been previously implicated as triggers for heavy drinking. Our studies demonstrate that, in male mice, a history of chronic binge alcohol-drinking elevates BNST levels of the mGlu5-scaffolding protein Homer2 and activated extracellular signal-regulated kinase (ERK) in an adaptive response to limit alcohol consumption. Male and female transgenic mice expressing a point mutation of mGlu5 that cannot be phosphorylated by ERK exhibit excessive alcohol-drinking, despite greater behavioral signs of alcohol intoxication and reduced anxiety, and are insensitive to local manipulations of signaling in the BNST. These transgenic mice also show selective insensitivity to alcohol-aversion and increased novelty-seeking, which may be relevant to excessive drinking. Further, the insensitivity to alcohol-aversion exhibited by male mice can be mimicked by the local inhibition of ERK signaling within the BNST. Our findings elucidate a novel mGluR5-linked signaling state within BNST that plays a central and unanticipated role in excessive alcohol consumption.SIGNIFICANCE STATEMENTThe bed nucleus of the stria terminalis (BNST) is part of the limbic-hypothalamic system important for behavioral responses to stress and alcohol, and glutamate transmission within BNST is implicated in the neurobiology of alcoholism. The present study provides evidence that a history of excessive alcohol drinking increases signaling through the metabotropic glutamate receptor 5 (mGlu5) receptor within the BNST in an adaptive response to limit alcohol consumption. In particular, disruption of mGlu5 phosphorylation by extracellular signal-regulated kinase within this brain region induces excessive alcohol-drinking, which reflects a selective insensitivity to the aversive properties of alcohol intoxication. These data indicate that a specific signaling state of mGlu5 within BNST plays a central and unanticipated role in excessive alcohol consumption.
Heterogeneity of Activity-Induced Sodium Transients between Astrocytes of the Mouse Hippocampus and Neocortex: Mechanisms and Consequences
Authors: Ziemens Daniel , Oschmann Franziska , Gerkau Niklas J. , Rose Christine R. 
Published: February 2019

Activity-related sodium transients induced by glutamate uptake represent a special form of astrocyte excitability. Astrocytes of the neocortex, as opposed to the hippocampus proper, also express ionotropic glutamate receptors, which might provide additional sodium influx. We compared glutamate-related sodium transients in astrocytes and neurons in slices of the neocortex and hippocampus of juvenile mice of both sexes, using widefield and multiphoton imaging. Stimulation of glutamatergic afferents or glutamate application induced sodium transients that were twice as large in neocortical as in hippocampal astrocytes, despite similar neuronal responses. Astrocyte sodium transients were reduced by ∼50% upon blocking NMDA receptors in the neocortex, but not hippocampus. Neocortical, but not hippocampal, astrocytes exhibited marked sodium increases in response to NMDA. These key differences in sodium signaling were also observed in neonates and in adults. NMDA application evoked local calcium transients in processes of neocortical astrocytes, which were dampened upon blocking sodium/calcium exchange (NCX) with KB-R7943 or SEA0400. Mathematical computation based on our data predict that NMDA-induced sodium increases drive the NCX into reverse mode, resulting in calcium influx. Together, our study reveals a considerable regional heterogeneity in astrocyte sodium transients, which persists throughout postnatal development. Neocortical astrocytes respond with much larger sodium elevations to glutamatergic activity than hippocampal astrocytes. Moreover, neocortical astrocytes experience NMDA-receptor-mediated sodium influx, which hippocampal astrocytes lack, and which drives calcium import through reverse NCX. This pathway thereby links sodium to calcium signaling and represents a new mechanism for the generation of local calcium influx in neocortical astrocytes.SIGNIFICANCE STATEMENTAstrocyte calcium signals play a central role in neuron–glia interaction. Moreover, activity-related sodium transients may represent a new form of astrocyte excitability. Here we show that activation of NMDA receptors results in prominent sodium transients in neocortical, but not hippocampal, astrocytes in the mouse brain. NMDA receptor activation is accompanied by local calcium signaling in processes of neocortical astrocytes, which is augmented by sodium-driven reversal of the sodium/calcium exchanger. Our data demonstrate a significant regional heterogeneity in the magnitude and mechanisms of astrocyte sodium transients. They also suggest a close interrelation between NMDA-receptor-mediated sodium influx and calcium signaling through the reversal of sodium/calcium exchanger, thereby establishing a new pathway for the generation of local calcium signaling in astrocyte processes.
Regional Delta Waves In Human Rapid Eye Movement Sleep
Authors: Bernardi Giulio , Betta Monica , Ricciardi Emiliano , Pietrini Pietro , Tononi Giulio , Siclari Francesca 
Published: February 2019

Although the EEG slow wave of sleep is typically considered to be a hallmark of nonrapid eye movement (NREM) sleep, recent work in mice has shown that slow waves can also occur in REM sleep. Here, we investigated the presence and cortical distribution of negative delta (1–4 Hz) waves in human REM sleep by analyzing high-density EEG sleep recordings obtained in 28 healthy subjects. We identified two clusters of delta waves with distinctive properties: (1) a frontal-central cluster characterized by ∼2.5–3.0 Hz, relatively large, notched delta waves (so-called “sawtooth waves”) that tended to occur in bursts, were associated with increased gamma activity and rapid eye movements (EMs), and upon source modeling displayed an occipital-temporal and a frontal-central component and (2) a medial-occipital cluster characterized by more isolated, slower (<2 Hz), and smaller waves that were not associated with rapid EMs, displayed a negative correlation with gamma activity, and were also found in NREM sleep. Therefore, delta waves are an integral part of REM sleep in humans and the two identified subtypes (sawtooth and medial-occipital slow waves) may reflect distinct generation mechanisms and functional roles. Sawtooth waves, which are exclusive to REM sleep, share many characteristics with ponto-geniculo-occipital waves described in animals and may represent the human equivalent or a closely related event, whereas medial-occipital slow waves appear similar to NREM sleep slow waves.SIGNIFICANCE STATEMENTThe EEG slow wave is typically considered a hallmark of nonrapid eye movement (NREM) sleep, but recent work in mice has shown that it can also occur in REM sleep. By analyzing high-density EEG recordings collected in healthy adult individuals, we show that REM sleep is characterized by prominent delta waves also in humans. In particular, we identified two distinctive clusters of delta waves with different properties: a frontal-central cluster characterized by faster, activating “sawtooth waves” that share many characteristics with ponto-geniculo-occipital waves described in animals and a medial-occipital cluster containing slow waves that are more similar to NREM sleep slow waves. These findings indicate that REM sleep is a spatially and temporally heterogeneous state and may contribute to explaining its known functional and phenomenological properties.
Identification and Functional Testing of Novel Interacting Protein Partners for the Stress Sensors Wsc1p and Mid2p ofSaccharomyces cerevisiae
Authors: Santiago-Cartagena Ednalise , González-Crespo Sahily , Vélez Vladimir , Martínez Nelson , Snider Jamie , Jessulat Matthew , Aoki Hiroyuki , Minic Zoran , Akamine Pearl , Mejías Inoushka , Pérez Luis M , Rymond Brian C , Babu Mohan , Stagljar Igor , Rodríguez-Medina José R 
Published: February 2019

AbstractWsc1p and Mid2p are transmembrane signaling proteins of cell wall stress in the budding yeast Saccharomyces cerevisiae. When an environmental stress compromises cell wall integrity, they activate a cell response through the Cell Wall Integrity (CWI) pathway. Studies have shown that the cytoplasmic domain of Wsc1p initiates the CWI signaling cascade by interacting with Rom2p, a Rho1-GDP-GTP exchange factor. Binding of Rom2p to the cytoplasmic tail of Wsc1p requires dephosphorylation of specific serine residues but the mechanism by which the sensor is dephosphorylated and how it subsequently interacts with Rom2p remains unclear. We hypothesize that Wsc1p and Mid2p must be physically associated with interacting proteins other than Rom2p that facilitate its interaction and regulate the activation of CWI pathway. To address this, a cDNA plasmid library of yeast proteins was expressed in bait strains bearing membrane yeast two-hybrid (MYTH) reporter modules of Wsc1p and Mid2p, and their interacting preys were recovered and sequenced. 14 previously unreported interactors were confirmed for Wsc1p and 29 for Mid2p. The interactors’ functionality were assessed by cell growth assays and CWI pathway activation by western blot analysis of Slt2p/Mpk1p phosphorylation in null mutants of each interactor under defined stress conditions. The susceptibility of these strains to different stresses were tested against antifungal agents and chemicals. This study reports important novel protein interactions of Wsc1p and Mid2p that are associated with the cellular response to oxidative stress induced by Hydrogen Peroxide and cell wall stress induced by Caspofungin.
Ion Channel Contributions to Wing Development inDrosophila melanogaster
Authors: George Laura Faith , Pradhan Sarala Joshi , Mitchell Danae , Josey Megan , Casey Justin , Belus Matthew T , Fedder Karlie N , Dahal Giri Raj , Bates Emily Anne 
Published: February 2019

AbstractDuring morphogenesis, cells communicate with each other to shape tissues and organs. Several lines of recent evidence indicate that ion channels play a key role in cellular signaling and tissue morphogenesis. However, little is known about the scope of specific ion-channel types that impinge upon developmental pathways. The Drosophila melanogaster wing is an excellent model in which to address this problem as wing vein patterning is acutely sensitive to changes in developmental pathways. We conducted a screen of 180 ion channels expressed in the wing using loss-of-function mutant and RNAi lines. Here we identify 44 candidates that significantly impacted development of the Drosophila melanogaster wing. Calcium, sodium, potassium, chloride, and ligand-gated cation channels were all identified in our screen, suggesting that a wide variety of ion channel types are important for development. Ion channels belonging to the pickpocket family, the ionotropic receptor family, and the bestrophin family were highly represented among the candidates of our screen. Seven new ion channels with human orthologs that have been implicated in human channelopathies were also identified. Many of the human orthologs of the channels identified in our screen are targets of common general anesthetics, anti-seizure and anti-hypertension drugs, as well as alcohol and nicotine. Our results confirm the importance of ion channels in morphogenesis and identify a number of ion channels that will provide the basis for future studies to understand the role of ion channels in development.
Utilização do diodo emissor de luz (led) na cicatrização de queimaduras: revisão sistemática da literatura
Authors: Araújo Marlise de Jesus Santos , Martins Gabriela Botelho 
Published: February 2019

INTRODUÇÃO: O tratamento de vítimas de queimadura é desafiador pela gravidade da própria lesão e das complicações que podem ocorrer durante o processo de cicatrização. A fisioterapia dermatofuncional tem atuação no tratamento de cicatrizes, utilizando de terapias para acelerar o processo cicatricial e recuperação funcional adequada. O diodo emissor de luz ou LED tem sido descrita como uma possibilidade terapêutica valiosa para fisioterapia com o intuito de reduzir complicações funcionais e estéticas em processos cicatriciais de indivíduos queimados. OBJETIVOS: Revisar a literatura acerca da utilização da luz LED e seus efeitos na cicatrização tecidual na lesão por queimadura. MATERIAIS E MÉTODOS: Trata-se de uma revisão sistemática da literatura, realizada no mês de agosto de 2017, utilizando as bases de dados Bireme, PubMed e PeDro, baseado nos critérios PRISMA. Foram incluídos no estudo os artigos que abordassem a utilização do LED na cicatrização específica de queimaduras, publicados nos últimos cinco anos (2012 -2017), nos idiomas inglês e português. Os critérios de exclusão foram artigos que abordaram o uso do LED na cicatrização tecidual em geral. A seleção dos artigos foi realizada por dois autores, não havendo consenso entre ambos, um terceiro autor seria convocado, o que não foi necessário nesta busca. RESULTADOS: Foram encontrados 1.029 artigos na busca, destes foram incluídos 04 artigos, que abordaram a utilização do LED na cicatrização específica de queimaduras publicados de 2012 a 2017. Todos os estudos encontraram melhoras do processo cicatricial com uso do LED, mesmo sendo utilizados comprimentos de onda distintos nos trabalhos analisados. CONCLUSÃO: A utilização do LED no tratamento de queimaduras demonstrou favorecer o processo de cicatrização tecidual em animais e melhorar os aspectos histológicos nos tecidos tratados, porém ainda pouco utilizado na Fisioterapia.
Expression level of androgen receptors in tumor tissue and its prognostic significance in primary operable luminal breast cancer without overexpression of Her2/neu in postmenopausal women
Authors: Kit O I , Shatova Yu S , Tokmakov V V , Novikova I A , Ul’yanova E P , Zlatnik E Yu 
Published: February 2019

Aim. To evaluate the expression level and prognostic significance of androgen in primary operable luminal breast cancer without overexpression of Her2/neu in postmenopausal women.
Methods. We analyzed treatment outcomes of 60 cases of primary operable (T1-2N0M0) luminal breast cancer without overexpression of Her2/neu in postmenopausal women. The follow-up period was 5 years. All cases were divided by immunohistochemical method into luminal A (20 females) and luminal B (40 patients) subtypes. Along with the standard panel of immunohistochemical markers, expression of nuclear androgen receptors was measured in tumor tissues of all patients. Depending on the expression levels, patients with luminal A and B subtypes were divided into three groups: (1) with high, (2) moderate and (3) low or negative expression.
Results. Mean levels of androgen receptor expression in the nuclei of tumor cells in patients with luminal A subtype (57.3±5.9%) were higher than those of luminal B subtype (21.4±4.04%) by 62.7% (Mann-Whitney test, р=0.0026). In patients with luminal A subtype, the maximal accumulation of androgen receptors in the nuclei of tumor cells was 2.7 times higher (р=0.0023) than in patients with luminal B subtype. All cases diagnosed with the disease progression were characterized by low or negative level of nuclear androgen receptor expression.
Conclusion. Negative and low levels of androgen receptor expression in tumor tissues of postmenopausal patients with luminal primary operable breast cancer without overexpression of Her2/neu might be an independent factor associated with poor prognosis.
Role of genus Candida in etiology of nosocomial infections in a multidisciplinary surgical clinic
Authors: Atakishizade S A 
Published: February 2019

Aim. To establish the role of genus Candida in etiology of nosocomial infections in a multidisciplinary surgical clinic of Azerbaijan Medical University in 2014-2016.
Methods. Microbiological study was performed on the pathological material from 41 patients with nosocomial pneumonia, 40 patients with surgical site infections and 46 patients with nosocomial sepsis.
Results. Nosocomial infections developing in a multidisciplinary surgical clinic were found to depend on localization of nosocomial infections. In nosocomial pneumonia 24% of all isolated microorgamisms were fungi of genus Candida among which C. albicans was prevailing: 9 of 12 isolated strains of Candida (75%) were identified as C. albicans. In patients with surgical site infections 10.7% of all isolated microorganisms were fungi of the genus Candida among which C. albicans was also prevailing: 4 of 6 isolated strains of Candida (66.7%) were identified as C. albicans. The isolated strains of Candida were sensitive to the studied antifungal medicines (amphotericin B, fluconazole, voriconazole, ketokonazole, nystatin).
Conclusion. In nosocomial infection and surgical site infections Candida fungi constituted 10.7-24% of all microorganisms isolated from the patients; the isolated Candida fungi - causative agents of nosocomial infections - were not resistant to antifungal medicines (amphotericin B, fluconazole, voriconazole, ketokonazole, nystatin).