bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chimeric antigen receptor macrophage therapy for breast tumours mediated by targeting the tumour extracellular matrix
Authors: Zhang Wenlong , Liu Ling , Su HuiFang , Liu Qin , Shen Jie , Dai Hanren , Zheng Wei , Lu Yan , Zhang Weijie , Bei Yuncheng , Shen Pingping 
Published: October 2019

AbstractBackgroundThe extracellular matrix (ECM) is essential for malignant tumour progression, as it is a physical barrier to various kinds of anticancer therapies. Matrix metalloproteinase (MMPs) can degrade almost all ECM components, and macrophages are an important source of MMPs. Studies using macrophages to treat tumours have shown that macrophages can enter tumour tissue to play a regulatory role.MethodsWe modified macrophages with a designed chimeric antigen receptor (CAR), which could be activated after recognition of the tumour antigen HER2 to trigger the internal signalling of CD147 and increase the expression of MMPs.ResultsAlthough CAR-147 macrophage treatment did not affect tumour cell growth in vitro compared with control treatment. However, we found that the infusion of CAR-147 macrophages significantly inhibited HER2-4T1 tumour growth in BALB/c mice. Further investigation showed that CAR-147 macrophages could reduce tumour collagen deposition and promote T-cell infiltration into tumours, which were consistent with expectations. Interestingly, the levels of the inflammatory cytokines TNF-α and IL-6, which are key factors in cytokine release syndrome, were significantly decreased in the peripheral blood in CAR-147 macrophage-transfused mice.ConclusionOur data suggest that targeting the ECM by engineered macrophages would be an effective treatment strategy for solid tumours.
Highly CO2-permeable membranes derived from a midblock-sulfonated multiblock polymer after submersion in water
Authors: Dai Zhongde , Deng Jing , Aboukeila Hesham , Yan Jiaqi , Ansaloni Luca , Mineart Kenneth P. , Giacinti Baschetti Marco , Spontak Richard J. , Deng Liyuan 
Published: October 2019

AbstractTo mitigate the effect of atmospheric CO2 on global climate change, gas separation materials that simultaneously exhibit high CO2 permeability and selectivity in gas mixtures must be developed. In this study, CO2 transport through midblock-sulfonated block polymer membranes prepared from four different solvents is investigated. The results presented here establish that membrane morphology and accompanying gas transport properties are sensitive to casting solvent and relative humidity. We likewise report an intriguing observation: submersion of these thermoplastic elastomeric membranes in liquid water, followed by drying prior to analysis, promotes not only a substantial change in membrane morphology, but also a significant improvement in both CO2 permeability and CO2/N2 selectivity. Measured CO2 permeability and CO2/N2 selectivity values of 482 Barrer and 57, respectively, surpass the Robeson upper bound, indicating that these nanostructured membranes constitute promising candidates for gas separation technologies aimed at CO2 capture.
Integrated analysis of transcriptomic and proteomic data from tree peony (P. ostii) seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism
Authors: Wang Xiaojing , Liang Haiying , Guo Dalong , Guo Lili , Duan Xiangguang , Jia Qishi , Hou Xiaogai 
Published: October 2019

Abstract
Tree peony (Paeonia section Moutan DC.) seeds are an excellent source of beneficial natural compounds that promote health, and they contain high levels of alpha-linolenic acid (ALA). In recent years, tree peony has been emerging as an oil crop. Therefore, combined analysis of the transcriptome and proteome of tree peony (P. ostii) seeds at 25, 32, 39, 53, 67, 81, 88, 95, and 109 days after pollination (DAP) was conducted to better understand the transcriptional and translational regulation of seed development and oil biosynthesis. A total of 38,482 unigenes and 2841 proteins were identified. A total of 26,912 differentially expressed genes (DEGs) and 592 differentially expressed proteins (DEPs) were clustered into three groups corresponding to the rapid growth, seed inclusion enrichment and conversion, and late dehydration and mature stages of seed development. Fifteen lipid metabolism pathways were identified at both the transcriptome and proteome levels. Pathway enrichment analysis revealed that a period of rapid fatty acid biosynthesis occurred at 53–88 DAP. Furthermore, 211 genes and 35 proteins associated with the fatty acid metabolism pathway, 63 genes and 11 proteins associated with the biosynthesis of unsaturated fatty acids (UFAs), and 115 genes and 24 proteins associated with ALA metabolism were identified. Phylogenetic analysis revealed that 16 putative fatty acid desaturase (FAD)-encoding genes clustered into four FAD groups, eight of which exhibited the highest expression at 53 DAP, suggesting that they play an important role in ALA accumulation. RT-qPCR analysis indicated that the temporal expression patterns of oil biosynthesis genes were largely similar to the RNA-seq results. The expression patterns of fatty acid metabolism- and seed development-related proteins determined by MRM were also highly consistent with the results obtained in the proteomic analysis. Correlation analysis indicated significant differences in the number and abundance of DEGs and DEPs but a high level of consistency in expression patterns and metabolic pathways. The results of the present study represent the first combined transcriptomic and proteomic analysis of tree peony seeds and provide insight into tree peony seed development and oil accumulation.
Interpretable deep learning for guided microstructure-property explorations in photovoltaics
Authors: Pokuri Balaji Sesha Sarath , Ghosal Sambuddha , Kokate Apurva , Sarkar Soumik , Ganapathysubramanian Baskar 
Published: October 2019

AbstractThe microstructure determines the photovoltaic performance of a thin film organic semiconductor film. The relationship between microstructure and performance is usually highly non-linear and expensive to evaluate, thus making microstructure optimization challenging. Here, we show a data-driven approach for mapping the microstructure to photovoltaic performance using deep convolutional neural networks. We characterize this approach in terms of two critical metrics, its generalizability (has it learnt a reasonable map?), and its intepretability (can it produce meaningful microstructure characteristics that influence its prediction?). A surrogate model that exhibits these two features of generalizability and intepretability is particularly useful for subsequent design exploration. We illustrate this by using the surrogate model for both manual exploration (that verifies known domain insight) as well as automated microstructure optimization. We envision such approaches to be widely applicable to a wide variety of microstructure-sensitive design problems.
Biological and antibacterial properties of the micro-nanostructured hydroxyapatite/chitosan coating on titanium
Authors: Li Baoe , Xia Xiaomei , Guo Miaoqi , Jiang Yu , Li Yu , Zhang Zhiyuan , Liu Shimin , Li Haipeng , Liang Chunyong , Wang Hongshui 
Published: October 2019

AbstractTitanium (Ti) is the widely used implant material in clinic, however, failures still frequently occur due to its bioinertness and poor antibacterial property. To improve the biological and antibacterial properties of Ti implants, micro-nanostructured hydroxyapatite (HA) coating was prepared on Ti surface by micro-arc oxidation (MAO), and then the antibacterial agent of chitosan (CS) was loaded on the HA surface through dip-coating method. The results showed that the obtained HA/CS composite coating accelerated the formation of apatite layer in SBF solution, enhanced cell adhesion, spreading and proliferation, and it also inhibited the bacterial growth, showing improved biological and antibacterial properties. Although, with the increased CS amount, the coverage of HA coating would be enlarged, resulting in depressed biological property, however, the antibacterial property of the composite coating was enhanced, and the cytotoxicity about CS was not detected in this work. In conclusion, the HA/CS coating has promising application in orthopedics, dentistry and other biomedical devices.
Unbalanced Expression of ICOS and PD-1 in Patients with Neuromyelitis Optica Spectrum Disorder
Authors: Xue Qun , Li Xiaoping , Gu Yanzheng , Wang Xiaozhu , Wang Mingyuan , Tian Jingluan , Duan Xiaoyu , Gao Hanqing , Ji Xiaopei , Yan Xiaoming , Dong Wanli , Fang Qi , Zhang Xueguang 
Published: October 2019

AbstractNeuromyelitis optica spectrum disorder (NMOSD) likely results from humoral immune abnormalities. The role that helper T cells play in the pathogenesis of this disease is not fully understood. To ascertain the clinical significance of two important costimulatory molecules required for T-cell activation in the peripheral blood of patients with NMOSD, we examined the expression levels of a membrane- and soluble-type inducible costimulatory molecule (ICOS), its ligand (ICOSL), programmed death-1 (PD-1), and its ligand (PD-L1) in the peripheral blood of 30 patients with NMOSD and compared these levels with those in patients with longitudinally extensive transverse myelitis (LETM), those with optic neuritis (ON), and healthy controls (HCs). Our results showed that the ICOS/ICOSL and PD-1/PD-L1 pathways may play important roles in the early stages of NMOSD pathogenesis. ICOS and PD-1 are potential therapeutic targets and valuable biomarkers for the differential diagnosis of early-stage NMOSD.
Paradoxical epigenetic regulation of XAF1 mediates plasticity towards adaptive resistance evolution in MGMT-methylated glioblastoma
Authors: Wu Qiong , Berglund Anders E. , Wang Dapeng , MacAulay Robert J. , Mulé James J. , Etame Arnold B. 
Published: October 2019

AbstractEpigenetic regulation of O6-alkylguanine DNA alkyltransferase (MGMT) is surrogate of intrinsic resistance to temozolomide (TMZ). However, mechanisms associated with adaptive resistance evolution of glioblastoma (GBM) relative to MGMT methylation remain unclear. We hereby report a paradoxical yet translational epigenetic regulation of plasticity towards adaptive resistance in GBM. Based on an adaptive resistance model of GBM cells with differential MGMT methylation profiles, MGMT-hypermethylation enhanced genetic and phenotypic plasticity towards adaptive resistance to TMZ while MGMT hypomethylation limited plasticity. The resulting model-associated adaptive resistance gene signature negatively correlated with GBM patient survival. XAF1, a tumor suppressor protein, paradoxically emerged as a mediator of differential plasticities towards adaptive resistance to TMZ through epigenetic regulation. XAF1 promoted resistance both in-vitro and in-vivo. Furthermore, XAF1 expression negatively correlated with XAF1 promoter methylation status, and negatively correlate with GBM patient survival. Collectively, XAF1 appears to have a pradoxical yet translational role in GBM.
Cataract surgery reduces intraocular pressure but not posture-induced intraocular pressure changes in patients with angle-closure glaucoma
Authors: Chang Pei-Yao , Wang Jia-Kang , Weng Hsin-Yu , Chang Shu-Wen 
Published: October 2019

AbstractCataract surgery leads to a sustained decrease in sitting intraocular pressure (IOP) in patients with angle-closure glaucoma (ACG). The purpose of this study is to evaluate whether cataract surgery can also reduce postural IOP changes. We prospectively examined 106 eyes from 53 patients with narrow angles scheduled for phacoemulsification. IOP was measured in the sitting, supine, and lateral decubitus positions using an ICare rebound tonometer before and 1 week, 1 month, and 3 months postoperatively. The mean baseline IOP in the sitting and lateral decubitus positions was 17.9 ± 4.8 mmHg and 21.43 ± 6.44 mmHg, which significantly reduced to 13.52 ± 3.8 and 17.46 ± 3.62, respectively, 3 month postoperatively (p < 0.001). However, postural IOP change (lateral decubitus minus sitting) at 3 months postoperatively was not significantly different from that at the baseline (3.17 ± 2.63 vs. 3.53 ± 3.38 mmHg, p = 0.85). Postural IOP change was not associated with preoperative sitting IOP, anterior chamber depth, axial length, fixed pupil, or presence of glaucomatous optic neuropathy. Patients with higher preoperative IOP exhibited greater IOP reduction after cataract surgery in every posture (p < 0.0001). In conclusion, cataract surgery reduces IOP in all postures among patients with ACG; however, it does not reduce the magnitude of postural IOP change.
Quantitative analysis of three-dimensional morphology and membrane dynamics of red blood cells during temperature elevation
Authors: Jaferzadeh Keyvan , Sim MinWoo , Kim NamGon , Moon InKyu 
Published: October 2019

AbstractThe optimal functionality of red blood cells is closely associated with the surrounding environment. This study was undertaken to analyze the changes in membrane profile, mean corpuscular hemoglobin (MCH), and cell membrane fluctuations (CMF) of healthy red blood cells (RBC) at varying temperatures. The temperature was elevated from 17 °C to 41 °C within a duration of less than one hour, and the holograms were recorded by an off-axis configuration. After hologram reconstruction, we extracted single RBCs and evaluated their morphologically related features (projected surface area and sphericity coefficient), MCH, and CMF. We observed that elevating the temperature results in changes in the three-dimensional (3D) profile. Since CMF amplitude is highly correlated to the bending curvature of RBC membrane, temperature-induced shape changes can alter CMF’s map and amplitude; mainly larger fluctuations appear on dimple area at a higher temperature. Regardless of the shape changes, no alterations in MCH were seen with temperature variation.
Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses
Authors: Jin Xiaofang , Cao Dan , Wang Zhongjie , Ma Linlong , Tian Kunhong , Liu Yanli , Gong Ziming , Zhu Xiangxiang , Jiang Changjun , Li Yeyun 
Published: October 2019

AbstractLate embryogenesis abundant (LEA) proteins are widely known to be present in higher plants and are believed to play important functional roles in embryonic development and abiotic stress responses. However, there is a current lack of systematic analyses on the LEA protein gene family in tea plant. In this study, a total of 48 LEA genes were identified using Hidden Markov Model profiles in C. sinensis, and were classified into seven distinct groups based on their conserved domains and phylogenetic relationships. Genes in the CsLEA_2 group were found to be the most abundant. Gene expression analyses revealed that all the identified CsLEA genes were expressed in at least one tissue, and most had higher expression levels in the root or seed relative to other tested tissues. Nearly all the CsLEA genes were found to be involved in seed development, and thirty-nine might play an important role in tea seed maturation concurrent with dehydration. However, only sixteen CsLEA genes were involved in seed desiccation, and furthermore, most were suppressed. Additionally, forty-six CsLEA genes could be induced by at least one of the tested stress treatments, and they were especially sensitive to high temperature stress. Furthermore, it was found that eleven CsLEA genes were involved in tea plant in response to all tested abiotic stresses. Overall, this study provides new insights into the formation of CsLEA gene family members and improves our understanding on the potential roles of these genes in normal development processes and abiotic stress responses in tea plant, particularly during seed development and desiccation. These results are beneficial for future functional studies of CsLEA genes that will help preserve the recalcitrant tea seeds for a long time and genetically improve tea plant.
The EDGI new take on EDGE: EUS-directed transgastric intervention (EDGI), other than ERCP, for Roux-en-Y gastric bypass anatomy: a multicenter study
Authors: Krafft Matthew R. , Hsueh William , James Theodore W. , Runge Thomas M. , Baron Todd H. , Khashab Mouen A. , Irani Shayan S. , Nasr John Y. 
Published: October 2019

Abstract
Background and study aims Indications for accessing the duodenum, and/or excluded stomach in Roux-en-Y gastric bypass (RYGB) patients extend beyond diagnosis and treatment of pancreaticobiliary maladies. Given the high technical and clinical success of EUS-directed transgastric ERCP (EDGE) in RYGB anatomy, we adopted this transgastric (anterograde) approach to evaluate and treat luminal and extraluminal pathology in and around the excluded gut in RYGB patients. EUS-directed transgastric intervention (“EDGI”), other than ERCP, is the terminology we have chosen to describe this heterogenous group of transgastric diagnostic and/or interventional endoscopic procedures (transgastric interventions) performed via a lumen-apposing mental stent (LAMS) in select patients with RYGB.
Patients and methods A multicenter (n = 4), retrospective study of RYGB patients with suspected luminal or extraluminal pathology, in or around the duodenum and/or excluded stomach, underwent EDGI using LAMS between December 2015 and January 2019.
Results A total of 14 patients (78.6 % women; mean age, 55.7 + 12.4 years) underwent EDGI via LAMS. Technical and clinical success rates of EDGI were 100 %. The most common transgastric interventions were diagnostic EUS of extraluminal pathology (n = 6, 42.7 %) and endoscopic biopsy of gastroduodenal luminal abnormalities (n = 5, 35.7 %). Two moderate-severity adverse events due to LAMS maldeployment occurred during EUS-JG creation (14.3 %), and each instance was successfully rescued with a bridging stent.
Conclusions A variety of gastroduodenal luminal and extraluminal disorders in RYGB patients can be effectively diagnosed and managed using EDGI via LAMS.
Diagnostic yield of Franseen and Fork-Tip biopsy needles for endoscopic ultrasound-guided tissue acquisition: a meta-analysis
Authors: Facciorusso Antonio , Del Prete Valentina , Buccino Vincenzo Rosario , Purohit Purvi , Setia Puneet , Muscatiello Nicola 
Published: October 2019

Abstract
Background and study aims Although newer needle designs are thought to improve diagnostic outcomes of endoscopic ultrasound-guided fine-needle biopsy, there is limited evidence on their diagnostic performance. The aim of this meta-analysis was to provide a pooled estimate of the diagnostic performance and safety profile of Franseen and Fork-tip fine-needle biopsy needles.
Patients and methods Computerized bibliographic search on the main databases was performed through March 2019. The primary endpoint was sample adequacy. Secondary outcomes were diagnostic accuracy, optimal histological core procurement, mean number of needle passes, pooled specificity and sensitivity. Safety data were also analyzed.
Results Twenty-four studies with 6641 patients were included and pancreas was the prevalent location of sampled lesions. Overall sample adequacy with the two newer needles was 94.8 % (93.1 % – 96.4 %), with superiority of Franseen needle over Fork-tip (96.1 % versus 92.4 %, P < 0.001). Sample adequacy in targeting pancreatic masses was 95.6% and both needles produced results superior to fine-needle aspiration (FNA) (odds ratio 4.29, 1.49 – 12.35 and 1.79, 1.01 – 3.19 with Franseen and Fork-tip needle, respectively). The rate of histological core procurement was 92.5%, whereas diagnostic accuracy and sensitivity were 95 % and 92.8 %, again with no difference between the two needles. Number of needle passes was significantly lower in comparison to FNA (mean difference: –0.42 with Franseen and –1.60 with Fork-tip needle). No significant adverse events were registered.
Conclusion Our meta-analysis speaks in favor of use of newer biopsy needles as a safe and effective tool in endoscopic ultrasound-guided tissue acquisition.
Medical Needling – Effekt auf hypertrophe Verbrennungsnarben hinsichtlich der Hautspannung und -elastizität
Authors: Busch Kay Hendrik , Aliu Antigona , Bender Richard , Walezko Nicole , Aust Matthias C. 
Published: October 2019

Zusammenfassung
Hintergrund Verbrennungsnarben stellen ein ernstzunehmendes physisches und psychisches Problem für die betroffenen Personen dar. Klinische Studien und wissenschaftliche Forschungen haben gezeigt, dass Medical Needling die Narbenbeschaffenheit hinsichtlich der Parameter Feuchtigkeit, Elastizität, transepidermalen Wasserverlust und Erythem verbessert. Gleichzeitig werden die Patienten mit einer vergleichsweise risikoarmen Therapie ohne postinterventionelle Komplikationen konfrontiert.
Ziel Ziel dieser Studie war es, den Einfluss von Medical Needling auf die Qualität ausgereifter hypertropher Narben hinsichtlich der Parameter Spannung und Elastizität zu testen. Insgesamt wurden 20 Patienten im Durchschnittsalter von 34,6 Jahren mit Verbrennungen zweiten und dritten Grades behandelt. Narben, welche mindestens 2 Jahre alt waren, wurden in unsere Studie involviert.
Methoden Medical Needling wird mit einem Roller durchgeführt, welcher mit Nadeln von 3 mm Länge besetzt ist. Mit konstantem Druck wird dieser vertikal, diagonal und horizontal über das zu behandelnde Hautareal gerollt. Die dermale Punktion verursacht Mikroläsionen mit anschließender minimaler, intradermaler Blutung. Dieser mechanische Prozess stimuliert mittels endogener Wachstumsfaktoren eine von der Dermis ausgehende Regeneration der Haut. Patienten wurden postoperativ bis zu 12 Monate nach der Behandlung anhand objektivierbarer Messverfahren untersucht. Gleichzeitig wurde der Parameter „Patientenzufriedenheit“ als subjektives, qualitatives Maß für eine Verbesserung der Narbenqualität erhoben.
Ergebnisse Die objektiven Messdaten ergaben, dass Medical Needling sowohl eine Reduktion der Spannung von straffen hypertrophen Narben, als auch eine erhöhte Festigkeit bei überdehnten Narben bewirkt. Tiefgreifende Läsionen mit Verlust elastischer Elemente bedingen rigide Narben, welche durch eine Behandlung mit Medical Needling an vitalen Eigenschaften sowie physiologischer Elastizität gewannen.
Schlussfolgerung Medical Needling erweist sich als ein vielversprechender Therapieansatz zur Behandlung hypertropher Narben mit pathologischen Veränderungen der Hautspannung und -elastizität.
Rehabilitation der brandverletzten Hand
Authors: Ziegenthaler Hans 
Published: October 2019

ZusammenfassungVerletzungen speziell an den Händen finden sich bei thermischen Unfällen
überproportional häufig. Ziel einer ICF-orientierten Rehabilitation des
Brandverletzten ist es, dem außergewöhnlich sensorisch und funktionell
entwickelten Greiforgan Hand möglichst viel an Funktionalität zurückzugeben bzw.
alltagspraktikable Kompensationsmechanismen zu erarbeiten.Dem anfangs bunten klinischen Bild mit Ödemen, Kontrakturen, Fehlstellungen sowie
hypertropher Narbenbildung und Epitheldefekten stehen vielfach hohe Erwartungen
an alltagsrelevante Funktionalität und Ästhetik des Brandverletzten
entgegen.Nur durch multimodale Therapieoptionen und ein interdisziplinäres Denken kann das
Reha-Team dieser besonderen Aufgabe gerecht werden.