bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transient up-regulation of miR-155-3p by lipopolysaccharide in primary human monocyte-derived macrophages results in RISC incorporation but does not alter TNF expression
Authors: Simmonds Rachel E. 
Published: March 2019

Background: The innate immune response is a tightly regulated process that reacts rapidly in response to pathogen-associated molecular patterns (PAMPs) such as lipopolysaccharide (LPS). Evidence is accumulating that microRNAs contribute to this, although few studies have examined the early events that constitute the “primary” response. Methods: LPS-dependent changes to miRNA expression were studied in primary human monocyte-derived macrophages (1°MDMs). An unbiased screen by microarray was validated by qPCR and a method for the absolute quantitation of miRNAs was also developed, utilising 5’ phosphorylated RNA oligonucleotide templates. RNA immunoprecipitation was performed to explore incorporation of miRNAs into the RNA-induced silencing complex (RISC). The effect of miRNA functional inhibition on TNF expression (mRNA and secretion) was investigated. Results: Of the 197 miRNAs expressed in 1°MDMs, only five were induced >1.5-fold. The most strongly induced was miR-155-3p, the partner strand to miR-155-5p, which are both derived from the BIC gene (B cell integration cluster, MIR155HG). The abundance of miR-155-3p was induced transiently ~250-fold at 2-4hrs and then returned towards baseline, mirroring the BIC mRNA. Other PAMPs, IL-1β, and TNF caused similar responses. IL-10, NF-κB, and JNK inhibition suppressed these responses, unlike cytokine-suppressing mycolactone. Absolute quantitation showed that miRNA abundance varies widely from donor-to-donor, and showed that miR-155-3p abundance is substantially less than miR-155-5p in unstimulated cells. However, at its peak there were 446-1,113 copies/cell, and miR-155-3p was incorporated into the RISC with an efficiency similar to miR-16-5p and miR-155-5p. Inhibition of neither miRNA affected TNF expression in 1°MDMs, but technical challenges here are noted. Conclusions: Dynamic regulation of miRNAs during the primary response is rare, with the exception of miR-155-3p, which transiently achieves levels that might have a biological effect. Further work on this candidate would need to overcome the technical challenges of the broad-ranging effects of liposomes on 1°MDMs.
Art(full) gifts: Material disruptions and conceptual proddings as creative acts of mentoring for early career art teachers
Authors: Hofsess Brooke Anne , Hanawalt Christina 
Published: March 2019

Abstract
Suspicious of the categories, limits and relations of power that typically define both mentoring of early career teachers and perceptions of beginning teacher identities, we set out to experiment with practices, concepts and materials that might afford new understandings and illuminate alternative practices. We explore art(full) gifts as creative acts of mentoring that attuned us to an empathetic and responsive recognition of the more-than-human ecology of teaching in public schools. In this ecology, currents of power, cultural and social norms, spaces and materials, perceptions, affects, memories, certification and training, and relationships ebb, flow, entangle and get stuck. This article, crafted in the epistolary spirit of our arts-based correspondence with early career art teachers, generates new insights and nuances regarding the interplay between mentoring and professional identities by asking: What might become of mentoring as practice and pedagogy when explored ecologically beyond a humanistic framework?
Juxtaposing professional identities and (re)telling pedagogical relations through experimental video-based research
Authors: Empain Joanna , Rifà-Valls Montserrat 
Published: March 2019

Abstract
This article focuses on art-based methodologies (ABR), particularly on practices based on the moving image such as experimental cinema/video and on how they can contribute to the construction of professional identities in art education and art-based educational research (ABER). As feminists, we locate ourselves in postcolonial visual culture studies and critical film/pedagogical theories. We explore how experimental video-based research, and auto-ethnographic video making, leads to a reflexive space in which to connect the artist–researcher–educator with the other bodies that participate in the research – María Ruido and a group of five fine art students. We focus our attention on the processes of narrating-editing as an autoethnographic and experimental way to produce the video narrative and the text Juxtaposition (2017). Finally, we show how the processes of narrating-editing enable the relocation of the different bodies, spaces and times and the juxtaposition of professional identities.
Management of Soil Fertility Based on Improvement Methodological Approach to Evaluation of Arable Land: Case of Ukraine
Authors: ULKO Yevhenii , KUCHER Anatolii , SALKOVA Iryna , PRIAMUKHINA Nataliia 
Published: March 2019

The article deals with the issues of the management of soil fertility based on the application of two methodological approaches to arable land normative monetary evaluation (NME). These researches have been conducted on the results of economic activity of the Poltava region agrarian enterprises of Ukraine. The differences between the two methodological approaches to the calculation of the NME of arable land in the Poltava region are shown. These approaches, namely, on cereals and on five agricultural crops, presuppose the calculation of the NME of arable land on the basis of capitalized rent income. The approaches are similar to the Ricardian model of land pricing, which is being widely used with its various modifications in different countries of the world. Our own methodology for analyzing the approaches to the NME of arable land, based on the developed stochastic harmonic models (SHM), is represented in the article.
The application of the two methodological approaches to the calculation of the NME of arable land in the Poltava region of Ukraine, which presupposes its consideration on the data concerning cereals and five agricultural crops, revealed essential differences between them in the course of the conducted economic-mathematical analysis. It has been found that the methodological approach on cereals has a lower level of the explanatory attribute variation (of humus content in soils) compared to five agricultural crops. That finally leads to the essential difference in the optimum values of the arable land NME at increasing humus content in soils. Thus, the optimum value of the arable land NME within the second methodological approach is twice as much as within the first one. The measures to improve the arable land NME, based on the data of the Poltava region and can be successfully used all over Ukraine, as well as in many countries of the world. The advantages of applying the methodological approach based on five agricultural crops were studied and grounded in the current research. This approach proves the dependence of arable land NME on the humus content and enables higher objectivity for effective and rational management of soil fertility.
Improvement of Technological Modernization Using Behavioral Economics
Authors: KUSSAINOVA Ademi , RAKHIMBERDINOVA Madina , DENISSOVA Oxana , TASPENOVA Gauhar , KONYRBEKOV Medet 
Published: March 2019

The purpose of this publication is to explain the applying of behavioral economics tools in the process of the technological upgrading of the Republic of Kazakhstan to be implemented. We need to: define the concept of behavioral economics; to reveal and describe in what way tools of behavioral economics may be applied, their nature and impact in the process of technological upgrading on the types of management; to understand and explain three principles of decision-making, the algorithm to make choice by people, profitable and unprofitable choices. Two main types of a feedback are provided and explained: reinforcing and balancing feedback.
In this article, it is required to find out what solutions are necessary for a post-industrial economy, what is constituted by methodological tools, which are required to implement the accelerated technological upgrading, and the subjects of organizational support of technological change.
URGENSI PENATAAN HUKUM KERUANGANGKASAAN DALAM KERANGKA KEPENTINGAN NASIONAL NEGARA BERKEMBANG
Authors: Pramono Agus 
Published: March 2019

The presence of the space industry which sends to be dominated by private companies in developed countries has encouraged the need for developing country national legal framework thar are accomodative to regulate commercial space activities. On the other hand there are developing countries that have space activities and have national legal instruments, on the other there are developing countries that have just started space activities but do not have national legal instrument. Therefore, the arrangement of international and national legal instrument that regulate the interest of developing countries is urgent. In addition, this study show that existing legal transformation is not successful considering the transformation is not less attention to the full interest of the parties concerned.
Combined evaluation of genotype and phenotype of thiopurine S-methyltransferase (TPMT) in the clinical management of patients in chronic therapy with azathioprine
Authors: Rucci Francesco , Cigoli Maria Sole , Marini Valeria , Fucile Carmen , Mattioli Francesca , Robbiano Luigi , Cavallari Ugo , Scaglione Francesco , Perno Carlo F. , Penco Silvana , Marocchi Alessandro 
Published: March 2019

Abstract

Background
The thiopurine S-methyltransferase (TPMT)/azathioprine (AZA) gene-drug pair is one of the most well-known pharmacogenetic markers. Despite this, few studies investigated the implementation of TPMT testing and the combined evaluation of genotype and phenotype in multidisciplinary clinical settings where patients are undergoing chronic therapy with AZA.

Methods
A total of 356 AZA-treated patients for chronic autoimmune diseases were enrolled. DNA was isolated from whole blood and the samples were analyzed for the c.460G>A and c.719A>G variants by the restriction fragment length polymorphism (RFLP) technique and sequenced for the c.238G>C variant. The TPMT enzyme activity was determined in erythrocytes by a high-performance liquid chromatography (HPLC) assay.

Results
All the patients enrolled were genotyped while the TPMT enzyme activity was assessed in 41 patients. Clinical information was available on 181 patients. We found no significant difference in the odds of having adverse drug reactions (ADRs) in wild-type patients and variant allele carriers, but the latter had an extra risk of experiencing hematologically adverse events. The enzyme activity was significantly associated to genotype.

Conclusions
TPMT variant allele carriers have an extra risk of experiencing hematologically adverse events compared to wild-type patients. Interestingly, only two out of 30 (6.6%) patients had discordant results between genotype, phenotype and onset of ADRs.
The Interaction between Erosion Particle and Gas Stream in High Temperature Gas Burner Rig for Thermal Barrier Coatings
Authors: Wu H. , Tan M. , Zhu W. , Ma Z. S. 
Published: March 2019

AbstractThermal barrier coatings (TBCs) as a kind of temperature-resistance materials have been widely applied in super high temperature components in aircraft engines. However, TBCs are subjected to harsh service environment such as high temperature oxidation and erosion, which lead to the coating failure. It is important to investigate the effect of fire temperature, angle and velocity of particle on erosion to understand the failure mechanism. In this paper, the temperature and velocity distributions of erosion particles in high temperature gas burner rig are investigated by using the fluid–solid coupling method with thediscrete random walk model. The results show that a non-uniform distribution of temperature appears in different positions of the central axis, and the temperature of particle is affected obviously by the gas stream and particle size. The trajectory of particles and velocity diagrams under different particle size are determined by coupling the continuous phase with the erosion particles.
Participation and attitudes towards gambling in Ghanaian youth: an exploratory analysis of risk and protective factors
Authors: Glozah Franklin N. , Tolchard Barry , Pevalin David J. 
Published: March 2019

Abstract

Purpose
There is little information on youth gambling in Ghana even though there is an unprecedented emergence of various types of gambling and gambling venues throughout the country. The aim of this cross-sectional exploratory study was to examine the role of perceived social difficulties and perceived protective social factors in participation and attitudes of Ghanaian youth towards gambling using data from a school-based survey (n = 770).

Methods
Participants completed measures on perceived social difficulties, perceived protective social factors, attitudes towards gambling and participation in four types of gambling.

Results
Sports betting was the most common form of gambling. There were significant differences in gambling participation with males gambling more than females. Youth who reported more perceived social difficulties from family and friends had a more positive attitude and a negative attitude towards gambling, respectively. Youth who reported higher protective social factors from family and teachers were less likely to have a negative attitude towards gambling. In all situations, high frequency gambling resulted in a more positive attitude towards gambling.

Conclusion
Perceived social difficulties influences Ghanaian youths to have a positive attitude towards gambling, however, protective social factors from family and teachers may help youth to have a negative attitude towards gambling, gamble less and consequently achieve academic success.
Origin, ecology and human conflict of gray wolf (Canis lupus) in Suleman Range, South Waziristan, Pakistan
Authors: Hamid Abdul , Mahmood Tariq , Fatima Hira , Hennelly Lauren Mae , Akrim Faraz , Hussain Abid , Waseem Muhammad 
Published: March 2019

Abstract
The gray wolf (Canis lupus; Linnaeus, 1758) is one of the most widespread terrestrial species and occurs in a variety of habitats. While well studied in North America and Europe, wolf populations in Asia are among the most evolutionarily distinct, endangered and data deficient. The Indian wolf (Canis lupus pallipes) is a subspecies of gray wolf that ranges from Southwest Asia to the Indian Subcontinent. Despite being categorized as “Endangered” in Pakistan, data on the gray wolf’s status, ecology and distribution are poorly understood. The current study investigates its genetic distinctiveness, distribution, feeding ecology and wolf livestock conflict in the Suleman Range, South Waziristan, Pakistan. We confirmed that the gray wolf is present in South Waziristan and is genetically similar to the wolves of Iran and Saudi Arabia based on their mtDNA D-loop haplotypes. The gray wolf was recorded at eight different sampling sites in the study area with elevational range between 1642 m to 2688 m. We estimated a population of 15 wolves, with a density of 0.62 individuals/km2 area surveyed. An analysis on scats revealed 52% contribution from livestock (with goats and sheep being the preferred prey) and 48% from wild prey. Biomass consumption showed gray wolf relied heavily on domestic prey (88%) during the summer season, resulting in human conflict with 28 wolves killed in response to livestock depredation during 2016–2017, requiring immediate conservation measures to save its remaining population.
Synthesis of a novel brominated vinylic fatty acid with antileishmanial activity that effectively inhibits the Leishmania topoisomerase IB enzyme mediated by halogen bond formation
Authors: Carballeira Néstor M. , Alequín Denisse , Lotti Diaz Leilani M. , Matos Victorio Jauregui , Ferreira Leonardo L. G. , Andricopulo Adriano D. , Golovko Mikhail Y. , Reguera Rosa M. , Pérez-Pertejo Yolanda , Balaña-Fouce Rafael 
Published: March 2019

Abstract
Many marine derived fatty acids, mainly from sponges, possess vinylic halogenated moieties (bromine or iodine) but their assessment as antileishmanial candidates remains elusive. In this work, we undertook the first total synthesis of a novel series of 2-allyl-3-halo-2-nonadecenoic acids, which preferentially inhibit the Leishmania DNA topoisomerase IB enzyme (LTopIB) over the human topoisomerase IB enzyme (hTopIB). The synthesis of 2-allyl-3-bromo-2E-nonadecenoic acid (1a) and 2-allyl-3-chloro-2E-nonadecenoic acid (2a) was achieved through a palladium catalyzed haloallylation of 2-nonadecynoic acid (2-NDA) using either allyl bromide or allyl chloride in the presence of PdCl2(PhCN)2 in 57–83 % overall yields. Among the new halogenated synthetic compounds, 1a was the most inhibitory of LTopIB with an EC50 = 7 μM, while the shorter chain analogs 2-allyl-3-bromo-2E-dodecenoic acid (1b) and 2-allyl-3-chloro-2E-dodecenoic acid (2b), synthesized from 2-dodecynoic acid, were not inhibitory of LTopIB (EC50 > 100 μM) resulting in the overall order of inhibition 1a > 2-NDA > 2a > > 1b ≅ 2b. The acids 1a and 2a inhibit LTopIB by a Gimatecan-independent mechanism. The enhanced LTopIB inhibition of 1a was computationally rationalized in terms of a halogen bond between the bromine in 1a and a DNA phosphate (binding energy = − 4.85 kcal/mol). Acid 1a also displayed preferential cytotoxicity towards Leishmania infantum amastigotes (EC50 = 2.5 μM) over L. infantum promastigotes (EC50 > 25 μM).
Overview on the use of surfactants for the preparation of porous carbon materials by the sol-gel method: applications in energy systems
Authors: Ortiz-Martínez Víctor Manuel , Gómez-Coma Lucía , Ortiz Alfredo , Ortiz Inmaculada 
Published: March 2019

Abstract
Porous carbon materials attract great interest because of the wide range of applications in electrochemical energy systems, especially in the case of structured and porosity-tuned carbons prepared by template-assisted methods. The use of surfactant prevents the collapse of the porous structure during the air-drying stage in the sol-gel process, which is regarded as a critical stage in this method. This work offers an overview on the use of surfactants as templates for the manufacture of tunable porous carbon materials by the sol-gel method mainly using the polymerization reaction of resorcinol (R) and formaldehyde (F). The use of surfactants avoids the application of other economically disadvantaged drying techniques such as supercritical fluids and freeze-drying. The surfactant-assisted sol-gel methods reported in the literature for the fabrication of porous carbons are widely discussed, as well as the potentiality of the synthesized materials as electrodes in electrochemical systems, which greatly depends on the final porous structure. Besides, this work offers information on hybrid methods in which surfactants are used not only for the fabrication of porous carbon materials with mesoporous/microporous structure but also for the development of advanced structures and composites, including nanomaterials with enhanced properties. Finally, future prospects in the synthesis of carbon materials prepared by surfactant-assisted sol-gel method are presented.
Narrating Disability in Literature and Visual Media: Introduction
Authors: Wohlmann Anita , Rana Marion 
Published: March 2019

AbstractThis introduction to the special issue attempts to map the intersections between disability studies on the one hand and literature and cultural studies on the other hand. We discuss concepts of disability as a social construction before we turn to literary and cultural approaches to disability, which involve controversies and questions about genre, narrative frames, recurring themes, and form. The last section gives an overview of how literary representations of disability resonate with life writing and identity theories.
The Original Pre-Priestly Account of the Post-Diluvian Age
Authors: Naʼaman Nadav 
Published: March 2019

Zusammenfassung
In diesem Artikel schlage ich vor, dass im Zyklus der vorpriesterschriftlichen Erzählungen die Geschichte des Turmbaus zu Babel nach der Fluterzählung stand und dass der priesterschriftliche Bearbeiter der Urgeschichte diese an ihren jetzigen Ort platziert hat, um einen Zusammenhang zwischen dem Ende der Urgeschichte und dem Beginn der Vätererzählungen herzustellen. Später fügte ein nachpriesterschriftlicher Bearbeiter die Episode zu Noahs Trunkenheit als Beginn der Zeit nach der Sintflut hinzu, womit die Botschaft lanciert wird, dass Kanaanäer von Beginn an verwünscht sind.
The Diachronic Composition of the Shema-Reports in Nehemiah 1–6
Authors: Rice John Will 
Published: March 2019

Zusammenfassung
In Neh 1–6 werden Berichte über Feinde Nehemias, die vom Fortschritt beim Bau der Stadtmauer Jerusalems »hören«, oft als alte narrative Trennlinien innerhalb des Textes verstanden. Die Ähnlichkeiten in der Form verdeutlichen die einheitliche Absicht der Berichte, die Abweichungen jedoch scheinen die Arbeit mehrerer Hände zu verraten. Dieser Aufsatz untersucht die Komposition dieser Verse anhand form- und redaktionkritischer Methoden und schlägt eine relative Chronologie vor, wobei die Verwendung oder Auslassung von Volksbezeichnungen für »Sanballat der Horonit«, »Tobiah der Ammonit« und »Geshem der Araber« hervorgehoben werden.
The Linguistic Connection between Antiochus IV Epiphanes and the »Abomination of Desolation« in the Greek Translations of the Book of Daniel
Authors: Scales Joseph 
Published: March 2019

Zusammenfassung
Dieser Artikel stützt die These, dass auf Antiochos IV. Epiphanes sprachlich in den griechischen Übersetzungen des Buches Daniel angespielt wird, so dass er mit der »Abscheulichkeit der Verwüstung« verbunden wird. Es ist wahrscheinlich, dass der Titel des Antiochus Ἐπιφανὴς bei der Verwendung von ἀφανίζω (vernichten, verwüsten) und seiner verwandten Nominalform im Hintergrund steht, insbesondere in der Theodotion-Übersetzung mit einigen anderen möglichen Referenzen im Altgriechischen.
Gottesname und Genitivattribut?
Authors: Stein Peter 
Published: March 2019

Zusammenfassung
Die Diskussion um den Charakter von »Aschera« in den Inschriften von Kuntillet ʿAǧrūd und Ḫirbet el-Kōm kreist um das Argument, die Determination eines Eigennamens durch ein Genitivattribut sei in der semitischen Grammatik nicht üblich. Bei der Suche nach Parallelen blieb eine semitische Schriftkultur bislang völlig außer Acht: Die Inschriften aus dem antiken Südarabien halten klare Beispiele für die Näherbestimmung eines Gottesnamens durch ein weiteres Theonym, aber auch durch ein suffigiertes Personalpronomen bereit, die bei der Beurteilung des althebräischen Befundes nicht unberücksichtigt bleiben sollten.
A Reanalysis of Psalm 11
Authors: Sumner Stephen T. 
Published: March 2019

Zusammenfassung
Eine Neubewertung von Psalm 11: 1d im masoretischen Text weicht von der traditionellen Wiedergabe (»zum Berg«) der alten Übersetzungen ab. Textliche Evidenzen, vergleichende literarische Analysen und lexikalische Überlegungen schließen eine terminative Interpretation von V. 1d aus und erfordern stattdessen eine ablative Übersetzung (»vom Berg«). Diese subtile Interpretationsverschiebung zu Beginn der Psalmen beeinflusst die Interpretation der gesamten Komposition und zeigt eine didaktische Funktion, die übersehen wurde. Die literarische Episode in V. 1–3 stellt sich eine königliche Figur vor, deren Hingabe an Jahwe von einem prophetischen Rat in Frage gestellt wird, der aber Zuversicht hinsichtlich der Erlösung durch Jahwe hat. V. 4–7 macht Mut zu einer ähnlichen Hingabe an Jahwe durch die königliche Figur. Die kultische und königliche Ideologie, die den gesamten Psalm durchzieht, spiegelt die Interessen des dafür verantwortlichen Liturgiker wider.