bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hospitalization Following Outpatient Diagnosis of Respiratory Syncytial Virus in Adults
Authors: Landi Suzanne N. , Garofalo Diana C. , Reimbaeva Maya , Scott Amie M. , Jiang Lili , Cappell Katherine , Lewandowski David , Bonafede Machaon , Brzozowski Kaylen , Drebert Zuzanna , Temple Mike , Tawadrous Margaret , Pixton Glenn C. , Alami Niki , Kelly Scott P. , Aliabadi Negar , Begier Elizabeth , Swan Joshua T. 
Published: November 2024

ImportanceRespiratory syncytial virus (RSV) is a leading cause of acute respiratory tract infections among adults and is estimated to cause approximately 159 000 hospitalizations among adults aged 65 years and older in the US each year. Estimates of hospitalization among adults with outpatient medically attended RSV (MA-RSV) infections are required to design interventional studies that aim to prevent hospitalization.ObjectiveTo assess absolute risk of 28-day, all-cause hospitalization following outpatient MA-RSV infections in adults.Design, Setting, and ParticipantsIn this cohort study, data from 3 different deidentified databases containing electronic health records (EHR) linked to closed claims data (Optum’s deidentified Integrated Claims-Clinical dataset, TriNetX Linked, and Veradigm Network EHR [VNEHR] database linked with claims) were analyzed separately across 6 RSV years (October 1, 2016, to September 30, 2022) in adults with commercial or government insurance. Outpatient (eg, clinics and emergency departments) MA-RSV infections were identified based on clinical laboratory data or RSV-specific International Statistical Classification of Diseases, Tenth Revision, Clinical Modification (ICD-10-CM) diagnosis codes. Data were analyzed from March 2023 to April 2024.Main Outcomes and MeasuresThe main outcome was all-cause 28-day hospitalization following outpatient MA-RSV infections among all adults and a high-risk subgroup (defined as age ≥65 years or with asthma, chronic obstructive pulmonary disease [COPD], or congestive heart failure [CHF]).ResultsIn this cohort study of 67 239 MA-RSV infections in adults (2771 from Optum, 7442 from TriNetX, and 57 026 from VNEHR), most occurred among females (62%-67%) and comorbidity prevalences were 20.0% to 30.5% for COPD, 14.6% to 24.4% for CHF, 14.6% to 24.4% for asthma; 14.0% to 54.5% of individuals were aged 65 years or older. The proportion hospitalized was 6.2% (95% CI, 5.3%-7.1%) in Optum, 6.0% (95% CI, 5.4% to 6.5%) in TriNetX, and 4.5% (95% CI, 4.3%-4.6%) in VNEHR. Among the high-risk subgroup, the proportion hospitalized was 7.6% (95% CI, 6.5%-8.9%) in Optum, 8.5% (95% CI, 7.6%-9.4%) in TriNetX, and 6.5% (95% CI, 6.2%-6.8%) in VNEHR.Conclusions and RelevanceIn this cohort study of adults with outpatient MA-RSV infections from 3 large deidentified US databases across 6 RSV seasons, approximately 1 in 20 adults experienced all-cause hospitalization within 28 days. The results of this study highlight the public health need for RSV prevention and treatment.
Dual‐Surface Polydentate Anchoring Enabled Strain Regulation for Stable and Efficient Perovskite Solar Cells
Authors: Zeng Fancong , Xu Lin , Hu Chencheng , Xing Jiahe , Wu Yanjie , Bai Xue , Dong Biao , Song Hongwei 
Published: November 2024

AbstractContinuous breakthroughs of photoelectric conversion efficiency (PCE) in perovskite solar cells are achieved, but the inherent instability caused by residual tensile strain and interfacial defects remains a major obstacle to their application. In this study, a polydentate ligand‐regulated dual‐surface stress management strategy for perovskite (PVK) is introduced to eliminate tensile strain and interface defects via multidentate anchoring. 3‐amino‐5‐bromopicolinaldehyde (BD) is employed on the lower surface of PVK, while its −CO, −NH2, and pyridine functional groups facilitate the bridging of SnO2 with PVK, alleviating tensile stress and lowering interfacial energy barriers. For the upper surface, the bis−SO2, pyridine, and bis−CF3 functional groups of N‐(5‐Chloro‐2‐pyridyl) bis(trifluoromethanesulfonimide) (FC) are utilized to increase the ion migration energy barrier through anchoring, which effectively diminishes tensile stress and defects. Besides, −CF3 also constructs a hydrophobic barrier on the upper surface. Notably, tensile stress successfully transforms into compressive stress based on the dual‐surface stress regulation, significantly improving the framework stability of PVK. Consequently, the devices treated with BD and FC achieve an elevated open‐circuit voltage of 1.24 V and PCE of 24.70%. The modified device (unencapsulated) maintains 92% of initial PCE after 2000 h in the atmosphere and 91% after 500 h under 85% RH, showcasing enhanced stability.
Rational Design of Advanced Gene Delivery Carriers: Macrophage Phenotype Matters
Authors: Wang Yue , Yao Yining , Zhang Yue , Yu Yingjie , Luo Jiangqi , Sweet Matthew J. , Yu Chengzhong 
Published: November 2024

AbstractNucleic acid delivery in hard‐to‐transfect macrophages have attracted increasing attention in diverse applications such as defence against bacterial infection. Regulated by microenvironments in specific applications, macrophages have a heterogenous nature and exist in different phenotypes with diverse functions, e.g., pro‐inflammatory and anti‐inflammatory. However, it is not clear whether macrophage phenotype affects nucleic acid delivery, and which one is harder to transfect, and the design of nucleic acid carriers in harder‐to‐transfect macrophage phenotypes is largely unexplored. Herein, it is first revealed that nucleic acid delivery efficacy in macrophages is influenced by phenotype: IL‐4‐treated “M2‐like” macrophages with suppressed mammalian target of rapamycin complex 1 (mTORC1) levels are harder‐to‐transfect than “M1‐like” macrophages for mRNA and DNA. This knowledge is then translated to the purpose‐design of gene delivery carriers for harder‐to‐transfect M2 phenotype macrophages dominant upon bacteria immune evasion. By loading chloroquine in tetrasulfide bond‐containing organosilica nanoparticles, the resultant composite promotes macrophage M2 polarization to M1 and increases mTORC1 levels for enhanced translation. The design is demonstrated in vitro and in vivo for pathogenic Escherichia coli (E. coli) and methicillin‐resistant Staphylococcus aureus (MRSA) infections. It is expected that the findings may provide new knowledge and gene delivery solutions in other applications where the M2 phenotype macrophage is dominant.
1D, 2D, and 3D Micropatterning of ZnO Nanoparticles and Nanorods by Two‐Photon Polymerization and Surface Functionalization
Authors: Ghabri Nawres , Gokarna Anisha , Issa Ali , Rumyantseva Anna , Kostcheev Sergei , Nomenyo Komla , Akil Suzanna , Maalej Ramzi , Lerondel Gilles , Jradi Safi 
Published: November 2024

AbstractIn this paper, a novel method is proposed for selective deposition of ZnO nanoparticles (NPs) and growth of ZnO Nanorods (NRs) on multi‐dimensional patterned polymer surfaces. The method involves microfabrication of functionalized 1D, 2D, and 3D polymer structures by two‐photon polymerization (TPP) followed by selective assembly of ZnO NPs acting as seeds before growing ZnO NRs by chemical bath deposition (CBD) technique at low temperature. The immobilization of the ZnO NPs seed layer is done by electrostatic interaction between the positively charged polymer surface functionalized by amine groups and the negatively charged ZnO NPs functionalized by citrate molecules. The influence of citrate molecules on the stability of the ZnO NPs dispersions and their immobilization on the polymer surface is demonstrated. Spatially controlled and selective growth of ZnO NRs is shown at low temperatures on multi‐dimensional structures up to 9 mm2 and having different shapes, including 1D polymer lines, 2D microplates, and 3D arrays of cones. The diameter of NRs and their orientation can be controlled through the size of ZnO NPs and the shape of the polymer structure respectively.
Synthesis of Functionalized Benzo[f]chromanes and Hydroxyalkyl Naphthols: Catalytic Coupling of Naphthols and Allylic Alcohols
Authors: Vijaya Sankar Raman , Gunanathan Chidambaram 
Published: November 2024

AbstractCatalytic dearomatization of arenols is an uphill task that can serve as a powerful method to construct C−C bonds with unsaturated coupling partners. Herein, a simple and efficient strategy for coupling naphthols with allylic alcohols is reported. A single Ru(II) pincer catalyzed coupling of naphthols with primary allylic alcohols led to the formation of benzo(f)chromanes, whereas the use of secondary alcohols delivered the hydroxyalkyl naphthols. Broad substrate scope and good functional group tolerance are demonstrated. Notably, a high diastereoselectivity is attained on chromanes. Hydroxyalkyl naphthols are synthetically transformed into spiroethers, and dearomative bromination is achieved on chromanes. Mechanistic studies revealed the involvement of tandem reactions, a formal O−H bond activation of allylic alcohols by an active catalyst via amine‐amide metal‐ligand cooperation provided α‐β, unsaturated carbonyl intermediates, which further underwent 1,4 conjugate addition with dearomatized naphthols. One of the crucial intermediates, naphthyl radical, is elucidated by EPR studies and trapped using a radical scavenger. Liberated hydrogen and water molecules are the only byproducts in these transformations.
Matrix Architecture and Mechanics Regulate Myofibril Organization, Costamere Assembly, and Contractility in Engineered Myocardial Microtissues
Authors: DePalma Samuel J. , Jilberto Javiera , Stis Austin E. , Huang Darcy D. , Lo Jason , Davidson Christopher D. , Chowdhury Aamilah , Kent Robert N. , Jewett Maggie E. , Kobeissi Hiba , Chen Christopher S. , Lejeune Emma , Helms Adam S. , Nordsletten David A. , Baker Brendon M. 
Published: November 2024

AbstractThe mechanical function of the myocardium is defined by cardiomyocyte contractility and the biomechanics of the extracellular matrix (ECM). Understanding this relationship remains an important unmet challenge due to limitations in existing approaches for engineering myocardial tissue. Here, they established arrays of cardiac microtissues with tunable mechanics and architecture by integrating ECM‐mimetic synthetic, fiber matrices, and induced pluripotent stem cell‐derived cardiomyocytes (iPSC‐CMs), enabling real‐time contractility readouts, in‐depth structural assessment, and tissue‐specific computational modeling. They found that the stiffness and alignment of matrix fibers distinctly affect the structural development and contractile function of pure iPSC‐CM tissues. Further examination into the impact of fibrous matrix stiffness enabled by computational models and quantitative immunofluorescence implicates cell‐ECM interactions in myofibril assembly, myofibril maturation, and notably costamere assembly, which correlates with improved contractile function of tissues. These results highlight how iPSC‐CM tissue models with controllable architecture and mechanics can elucidate mechanisms of tissue maturation and disease.
Sustained Endocytosis Inhibition via Locally‐Injected Drug‐Eluting Hydrogel Improves ADCC‐Mediated Antibody Therapy in Colorectal Cancer
Authors: Wu Chong , Liu Xiaoting , Liu Rong , Song Shiyao , Zheng Zi‐Fan , Zeng Yilin , Mei Yong , Zhang Jing‐Yang , Duan Qijia , Lin Run , Du Jin‐Zhi , He Weiling 
Published: November 2024

AbstractThe epidermal growth factor receptor (EGFR) is a validated therapeutic target for RAS/RAF wild‐type colorectal cancer (CRC). However, monoclonal antibody‐based anti‐EGFR therapies such as cetuximab have limited effectiveness. Herein, it is identified that EGFR internalization is associated with poor treatment response and prognosis in patients with CRC, based on a retrospective analysis of patients treated with cetuximab. It is further demonstrated that the endocytosis inhibitor prochlorperazine (PCZ) can move EGFR, which is hidden inside the cell, to the cell surface to improve therapeutic antibody binding. Thus, a thermosensitive hydrogel co‐loaded with cetuximab and PCZ (Gel@Cmab/PCZ) is constructed for sustained inhibition of endocytosis and effective cetuximab delivery. Peritumoral injection of Gel@Cmab/PCZ shows strong antitumor efficacy in subcutaneous and orthotopic CRC tumor models and completely abrogated liver metastasis when combined with chemotherapy. In a humanized patient‐derived xenograft model, a single injection of Gel@Cmab/PCZ with one‐third of the conventional cetuximab dose achieved 91% tumor growth inhibition, which promoted NK cell infiltration into tumor tissues and their antibody‐dependent cell‐mediated cytotoxicity effect. This study represents a novel strategy to boost the monoclonal antibody‐mediated anti‐tumor response in CRC.
scPharm: Identifying Pharmacological Subpopulations of Single Cells for Precision Medicine in Cancers
Authors: Tian Peng , Zheng Jie , Qiao Keying , Fan Yuxiao , Xu Yue , Wu Tao , Chen Shuting , Zhang Yinuo , Zhang Bingyue , Ambrogio Chiara , Wang Haiyun 
Published: November 2024

AbstractIntratumour heterogeneity significantly hinders the efficacy of anticancer therapies. Compared with drug perturbation experiments, which yield pharmacological data at the bulk cell level, single‐cell RNA sequencing (scRNA‐seq) technology provides a means to capture molecular heterogeneity at single‐cell resolution. Here, scPharm is introduced, a computational framework that integrates pharmacological profiles with scRNA‐seq data to identify pharmacological subpopulations of cells within a tumour and prioritize tailored drugs. scPharm uses the normalized enrichment scores (NESs) determined from gene set enrichment analysis to assess the distribution of cell identity genes in drug response‐determined gene lists. Based on the strong correlation between the NES and drug response at single‐cell resolution, scPharm successfully identified the sensitive subpopulations in ER‐positive and HER2‐positive human breast cancer tissues, revealed dynamic changes in the resistant subpopulation of human PC9 cells treated with erlotinib, and expanded its ability to a mouse model. Its superior performance and computational efficiency are confirmed through comparative evaluations with other single‐cell prediction tools. Additionally, scPharm predicted combination drug strategies by gauging compensation or booster effects between drugs and evaluated drug toxicity in healthy cells in the tumour microenvironment. Overall, scPharm provides a novel approach for precision medicine in cancers by revealing therapeutic heterogeneity at single‐cell resolution.
Long‐Term Health Outcomes of Individuals With Pseudodeficiency Alleles in IDUA May Inform Newborn Screening Practices for Mucopolysaccharidosis Type I
Authors: Grady Lauren O′ , Zoltick Emilie S. , Zouk Hana , He Wei , Perez Emma , Clarke Lorne , Gold Jessica , Strong Alanna , Sahai Inderneel , Yeo Julie , Green Robert C. , Karaa Amel , Gold Nina B. 
Published: November 2024

ABSTRACTMucopolysaccharidosis type I (MPS I), a lysosomal disorder caused by variants in IDUA, was added to the Recommended Uniform Screening Panel for newborn screening in 2016. Positive screening results for MPS I are commonly due to variants known as “pseudodeficiency alleles,” which decrease in vitro alpha‐L‐iduronidase enzyme activity but are thought to provide sufficient in vivo activity. Despite the historic assumption that these variants are biologically benign, the possibility that they could give rise to complex, multigenic, or attenuated phenotypes has not been systemically evaluated in adults. We completed a retrospective matched cohort study using a hospital‐based biorepository with data from 65,309 participants, we identified 1803 individuals harboring homozygous IDUA pseudodeficiency alleles. Using electronic medical records (EMR), we compared the prevalence of features of MPS I in participants with homozygous pseudodeficiency alleles to a cohort of matched control participants. We found no clinically relevant significant differences between cases and controls nor genotype–phenotype associations across four alleles. These findings provide empiric support that adults with homozygous IDUA pseudodeficiency alleles are unlikely to develop mild symptoms of disease compared with controls. This study provides a proof‐of‐concept model for other nonclassical disease variants related to other inherited metabolic disorders, which is necessary as newborn screening expands.
Integrating Sustainable Chemistry Principles in Polydiacetylene (PDA) Synthesis
Authors: Gendron David , Baillargeon Pierre 
Published: November 2024

AbstractPolydiacetylenes (PDAs) represent one of the most intriguing classes of semiconducting polymers, captivating researchers with their unique properties and diverse applications. They possess remarkable electronic and optical characteristics, as well as their ability to undergo dramatic color changes in response to various stimuli. This perspective explores the integration of sustainable chemistry principles into the synthesis of PDAs. We highlight how several elements such as diacetylene green‐coupling synthesis, biodegradability, solid state synthesis and biobased precursors, can contribute to the advancement of more responsible and innovative PDAs materials.
Exploring the Differences in Self‐Assembly Behavior, Structure, and Properties of Two Novel Pentanuclear Zinc (II) Salamo‐Type Complexes Possessing Solvent Effect
Authors: Zheng Ying‐Ru , Tuo Na , Zhang Hai‐Wei , Wang Li , Dong Wen‐Kui 
Published: November 2024

ABSTRACTA salamo‐type biscompartmental ligand was successfully prepared. On this basis, two novel pentanuclear zinc (II) salamo‐type complexes possessing solvent effect were self‐assembled successfully with different zinc (II) salts: [Zn5(L)2(μ‐OAc)2(CH3COCH3)] (1) and [Zn5(L)2(μ‐OAc)2(CH3CN)] (2). Complex 1 contains two fully deprotonated (L)4− units, five Zn(II) atoms, two coordinating acetate anions, and one coordinating acetone molecule. The zinc (II) atoms are all in a slightly distorted triangular bipyramidal geometrical configurations. Complex 2 consists of two fully deprotonated (L)4− units, five zinc (II) atoms, two coordinating acetate anions and a coordinating acetonitrile molecule. The different structures of the self‐assembling complexes may be attributed to various solvent effects applied in the self‐assembly. As a result, the acetate anions are involved in bridging coordination, thereby expanding the distance between secondary building units (SBUs). In addition, polar nonprotonic solvents, especially acetonitrile and acetone molecules, were involved in the coordination process. Finally, the reaction sites and intermolecular interactions were further discussed by MEP, IRI, and Hirshfeld surfaces, and their fluorescence properties were explored.
Green Synthesis of New Coumarins Employing SiO2/Fe3O4@MWCNTs as a Recyclable Catalyst and Evaluation of Antioxidant and Antiproliferative Activity
Authors: Hojjati Mahsa , Shikholeslami‐Farahani Fatemeh , Shahvelayati Ashraf Sadat , Hossaini Zinatossadat 
Published: November 2024

ABSTRACTThis study successfully synthesized coumarins, which belong to a novel class of fused heterocyclic compounds, employing a multicomponent reaction with excellent efficiency. The process utilized 4‐aminocumarin or 4‐hydroxycumarin, 2‐arylidenemalononitrile, and activated acetylenic chemicals in an aqueous solution at room temperature in the presence of MWCNTs@SiO2/Fe3O4 as a recyclable catalyst. The synthesized compounds were assessed for their antiproliferative activity against MCF‐7 breast and HCT‐15 colon cancer cell lines using the sulforhodamine B (SRB) assay, as well as their antioxidant effect. Compounds 4c, 4g, and 6d exhibited superior antiproliferative activity compared with other synthesized compounds. The compounds 4c, 4g, and 6d were subjected to UV‐Vis spectroscopy to determine their binding affinity for DNA and BSA. The analysis yielded overall affinity constants K4c‐DNA = 7.9 × 103 M−1, K4g‐DNA = 1.1 × 104 M−1, K6d‐DNA = 4.2 × 104 M−1, K4c‐BSA = 4.96 × 104 M−1, K4g‐BSA = 7.54 × 104 M−1, and K6d‐BSA = 6.47 × 104 M−1. Our research results could offer fresh data demonstrating the correlation between the chemical composition and the ability of these novel coumarin analogs to inhibit cancer growth. The synthesis of fused coumarins exhibited various beneficial characteristics, such as fast reactions, high yields of the end product, and easy isolation of the catalyst and product from the reaction mixture.
Synthesis, Structural Aspects, Magnetic, and Adsorption Properties of a Dinuclear Cu (II) Complex Derived From Mixed Ligand Approach
Authors: Akhtar Muhammad Nadeem , Aiman Umme , Qasim Muhammad Naveed , Atta Sawaira , Siddique Amna , AlDamen Murad A. , Bikas Rahman , Fitta Magdalena , Kozakiewicz‐Piekarz Anna 
Published: November 2024

ABSTRACTA dinuclear copper complex, [Cu2(teaH)(pNBA)2(H2O)2]·MeOH·pNBH (1) (where teaH3 = triethanolamine, pNBH = 4‐nitro‐benzoic acid, and pNBA = 4‐nitro‐benzoate) has been prepared and structurally characterized. In 1, there is an interesting intermolecular structure that is shown as a tetramer copper connected in chain‐like motif. In the UV‐Vis spectrum, higher absorbance at 267 nm is referred to n → π* transition, coupled with a weak peak at 500 nm, indicating another transition, which is specified as metal‐to‐ligand charge transfer. The higher intensity peak in the photoluminescence spectrum is marked as the absorption of energy required for the excitation of electrons, whereas lower intensity peaks show the emission of energy, which describes d‐d transitions of Cu (II) electrons due to d9 configuration. Complex 1 was explored for the adsorption of methylene blue (MB) dye, with the maximum adsorption as 101.07 mg/g, whereas the removal efficiency was estimated to be 81.05%. In conformity with the kinetic studies, the adsorption process proceeded via a Pseudo‐first‐order kinetic model. The incorporation of mixed ligands such as teaH3 and pNBH in 1 tends to increase the dimensionality that led to increased MB adsorption. The plausible mechanism behind the adsorption was favored by hydrogen‐bonding, electrostatic, π–π, and n–π* interactions, operating between the dye and complex 1. Further, the H‐bonding and these interactions provided stability to the complex, and an improved dye adsorption was observed even during the 2nd and 3rd recyclability experiments. Additionally, complex 1 corroborated remarkable stability after dye adsorption, allowing for up to four recycling turns. The magnetic study revealed antiferromagnetic coupling between the two magnetic centers with a singlet ground state (S = 0).
Mixed Perovskite Phases of BaTiO3/BaTi5O11 for Efficient Electrochemical Reduction of CO2 to CO
Authors: Marbaniang Phiralang , Kumar Tiwari Dilip , Ingavale Sagar , Lata Singh Deep , Rao G. Ranga 
Published: November 2024

AbstractOne of the most promising approaches in solving the energy crisis and reducing atmospheric CO2 emissions is artificial photosynthetic CO2 reduction. The electrochemical method for CO2 reduction is more appealing since it can be operated under ambient conditions, and the product selectivity strongly depends on the applied potential. Perovskites with ferroelectric properties strongly adsorb linear CO2 molecules. In this study, barium titanate (BaTiO3) perovskite is used as an electrocatalyst to promote CO2 activation and conversion to CO. Perovskite catalysts were prepared by ball‐milling followed by annealing at 900 °C for 4 to 6 h in an open atmosphere. The TEM and SEM study shows that the particle size varies in the range of 80–200 nm. Mixed phases of BaTiO3 and BaTi5O11 supported on nitrogen‐doped carbon nanotubes are found to be highly active for electrocatalytic CO2 reduction to CO with maximum Faradaic efficiency of 89.4 % at −1.0 V versus Ag/AgCl in CO2 saturated 0.5 KOH solution. This study concludes that mixed phases of BaTiO3 and BaTi5O11 are more active and highly selective for CO2 conversion to CO compared to single‐phase BaTiO3.