Abstract CT098: KEYNOTE-046: Effects of ADXS-PSA with or without pembrolizumab on survival and antigen spreading in metastatic, castration-resistant prostate cancer patients
Authors: Stein Mark , Fong Lawrence , Tutrone Ronald , Mega Anthony , Lam Elaine T. , Vangala Surya , Dennie Justin , Petit Robert , Gutierrez Andres , Hayes Sandy , Haas Naomi 
Published: August 2019

Abstract
Background: ADXS-PSA, an attenuated Listeria monocytogenes-based immunotherapy targeting prostate-specific antigen (PSA), is currently being evaluated as a treatment for progressive metastatic castration-resistant prostate cancer (mCRPC) in the Phase I/II KEYNOTE-046 trial as a monotherapy (Part A; PA) and in combination with pembrolizumab (Part B; PB).
Methods: This Phase I/II trial evaluated pts with mCRPC, ≥18 yrs who received ≤2 prior chemo-/targeted-/immunotherapies or ≤1 prior chemotherapy in a metastatic setting. Part A (PA; n = 13) pts received ADXS-PSA doses 1x109; 5 x109 or 1x1010 CFU IV every 3 wks and Part B (PB; n = 37) pts received 1x109 CFU + 200 mg pembro IV every 3 wks with a 4thpembro dose 3 wks later (in 12 wks cycles), for up to 2 yrs or until progression/toxicity. Safety/tolerability, antitumor activity and effect on PSA levels have been reported for both groups (Stein M et al., Keynote-046, ASCO 2018). Updates on OS and T cell reactivity to PSA and to other prostate cancer antigens (i.e., prostatic acid phosphatase (PAP), prostate-specific membrane antigen (PSMA), prostate stem cell antigen (PSCA), and prostein) are herein presented for PA and PB pts.
Results: At entry, PA and PB pts were ~70 yrs with a Gleason score &gt;8 and the majority had received prior abiraterone and/or enzalutamide. PB pts had higher median baseline (BL) PSA (40.6 vs. 20.8 ng/mL), and more prior enzalutamide (53 vs. 26%) and chemotherapy (49 vs. 36%) use vs PA pts. Overall, 2 PA (14%) v 16 PB pts (43%) had a decreased PSA post-BL. Of these, 7 PB (19%) vs. 0 PA pts achieved a confirmed PSA reduction ≥50% from BL. The median OS (months) in PB pts was 23 (17.4 -NR) vs. 8.5 (3.8-20.10) in PA pts. Median OS in PB pts with PSA reduction ≥50% from BL has not been reached as 100% (6/6) of pts are still alive. T cell reactivity, as measured by ELISpot assays, showed that 100% of PA (9/9) pts and 100% of PB (16/16) pts exhibited increases in the frequencies of T cells reactive to at least one prostate cancer antigen other than PSA, which is indicative of antigen spreading.
Conclusions: ADXS-PSA ± pembro elicited a broad antitumor T cell response in all mCRPC pts tested but only ADXS-PSA + pembro reduced PSA ≥50% from BL and prolonged OS in these select pts. Clinical trial information: NCT02325557.
Citation Format: Mark Stein, Lawrence Fong, Ronald Tutrone, Anthony Mega, Elaine T. Lam, Surya Vangala, Justin Dennie, Robert Petit, Andres Gutierrez, Sandy Hayes, Naomi Haas. KEYNOTE-046: Effects of ADXS-PSA with or without pembrolizumab on survival and antigen spreading in metastatic, castration-resistant prostate cancer patients [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr CT098.