bluuebunny/crossref_metadata_2025_split huggingface dataset:
Binding of Infectious Hypodermal and Haematopoietic Necrosis Virus‐Like Particles to Mannose Receptor Stimulates Antimicrobial Responses in Immune‐Related Tissues of Peneaus vannamei
Authors: Weerachatyanukul Wattana , Pooljun Chettupon , Chotwiwatthanakun Charoonroj , Jariyapong Pitchanee 
Published: November 2024

ABSTRACTMannose receptor (MR) is a transmembrane protein and a type of pattern‐recognition receptor (PRR) that plays a critical role in the immunity of mammals and fish. In this study, we examined the role of MR in binding with infectious hypodermal and haematopoietic necrosis virus‐like particle (IHHN‐VLP) and the downstream immune pathway that it triggers in the shrimp Peneaus vannamei. Upon IHHN‐VLP challenge, transcripts of MR in P. vannamei (PvMR) increased significantly in all examined tissues, particularly those related to shrimp immunity, including hemocyte, hepatopancreas and gill tissues. Specifically, IHHN‐VLP bound to the 34‐kDa PvMR protein in shrimp‐tissue extracts. Immunohistochemistry results of hemocytes showed that PvMR was initially localised on the plasma membrane but later internalised and dispersed throughout the cytoplasm after IHHN‐VLP administration. Binding between IHHN‐VLP and PvMR also induced significant upregulation of genes for the antimicrobial peptides (AMPs) penaeidin 3 and crustin, presumably to protect the shrimp against the viral infection. However, knocking down PvMR resulted in down‐regulation of all immune‐related genes examined. Overall, as an immune‐related PRR, PvMR serves as a receptor for invading viruses, which then trigger the expression of AMPs. Strategic designs using PvMR could be developed to either block the interaction of native virus with the host cells or provoke its up‐regulation to enhance shrimp immunity, which could open up opportunities to fight against IHHNV infection in shrimp.
Assessment of future risk of agricultural crop production under climate and social changes scenarios: A case of the Solo River basin in Indonesia
Authors: Shrestha Badri Bhakta , Rasmy Mohamed , Ushiyama Tomoki , Acierto Ralph Allen , Kawamoto Takatoshi , Fujikane Masakazu , Shinya Takafumi , Kubota Keijiro 
Published: November 2024

AbstractUnderstanding the impacts of climate change and conversion of paddy field areas in the future on agricultural production is an essential part of flood‐risk management. However, the quantitative impact of flood on agricultural crops in the far‐future under climate change, considering prospective changes in paddy area, is still not clearly understandable. This study thus focused on quantitative analysis of flood impact on rice crops under climate change using MRI‐AGCM climate model outputs for the past (1979–2002) and far‐future (2075–2098) periods for the Solo River basin in Indonesia. We developed a quantitative damage assessment method by coupling water and energy budget‐based rainfall‐runoff‐inundation model outputs and a depth‐duration‐damage flood loss model. We also analyzed land‐use and land cover changes to project future paddy areas. The future rice production in the study basin may decrease by 21% by 2048 and by 24.6% by 2076 compared with that in 2020, due to the conversion of paddy fields to other land cover classes. The average annual flood damage value of rice crops may increase in the future period (2075–2098) by 93.7% (average damage: 666.08 billion IDR) compared with that in the past period (1979–2002) (average damage: 343.7 billion IDR), due to climate change impacts alone.
Choice on the menu: Increasing meal choice for people living in residential aged care, a pilot study
Authors: Wheeler Mikaela , Abbey Karen L. , Capra Sandra M. 
Published: November 2024

AbstractBackgroundFor residents in residential aged care (RAC), opportunities to make decisions about their meals are often limited. Increasing choice for residents requires significant changes to be made to the foodservice system to deliver nutritionally adequate, timely and enjoyable meals to residents. The pilot project aimed to understand the effect of increasing choice in meals on residents, staff and the foodservice system.MethodsA pragmatic action research approach was used to collaboratively design and evaluate an altered a foodservice system that increased choice, enabling residents to place their order at the mealtime and choose from a menu of seven hot meal options for lunch and dinner. Outcome measures were measured pre‐ and post‐implementation and included food waste, resident satisfaction and foodservice costs.ResultsResident satisfaction measured on a 5‐point Likert scale improved between pre‐ (3.60 ± 1.09) and post‐ (4.57 ± 0.49) measurements, whereas production waste increased (pre—55g, post—90 g) and foodservice costs increased (pre—$9.20–$11.14 per resident per day, post—$11.01–$12.15 per resident per day). Compared to the standard cook serve meal, consumption of protein foods increased marginally (+5 g), vegetable consumption increased (+11 g) and carbohydrate consumption decreased (−38 g) for meals consumed from the intervention menu.ConclusionsIncreasing choice can have a positive impact of resident satisfaction; however, further work is needed to investigate how production waste and costs can be addressed.
Wild‐type transthyretin cardiac amyloidosis and aortic stenosis: Can carpal tunnel syndrome help distinguish the chicken from the egg?
Authors: Delbarre Marc‐Antoine , Chadha Gagan Deep , Annabi Mohamed‐Salah , Nouri Refaat , Zaroui Amira , Blanc‐Durand Paul , Rasolonirina Diana , Kharoubi Mounira , Bejan Ancuta , Galat Arnaut , Oghina Silvia , Pibarot Philippe , Tribouilloy Christophe , Damy Thibaud 
Published: November 2024

AbstractBackgroundThe frequent association between transthyretin wild‐type (TTRwt) cardiac amyloidosis (CA) and aortic stenosis (AS) suggests a bidirectional relationship: TTRwt‐CA could induce AS and vice versa. Systemic manifestations may highlight this interaction: systemic amyloidogenesis would lead to systemic symptoms, CA, and AS, whereas the myocardial stresses induced by degenerative AS might promote local amyloidogenesis without systemic symptoms. Carpal tunnel syndrome (CTS) is the most frequently reported extracardiac symptom. Through a comparison of TTRwt‐CA patients with and without CTS, we sought to determine whether CTS serves as a reliable indicator of systemic involvement and its impact on cardiac and valvular characteristics.Methods and resultsA total of 411 consecutive patients with TTRwt‐CA were included. CTS, present in 70.3%, was associated with a younger age (80 vs. 84 years, p < 0.001), more extracardiac symptoms, and advanced CA, with greater cardiac remodeling and a higher heart‐to‐mediastinum ratio (1.63 vs. 1.54; p = 0.012) compared to patients without CTS. AS was present in 21% and 31% of patients with and without CTS, respectively (p = 0.024). Except for AS, these associations remained significant after adjusting for confounding factors. In severe AS, patients with CTS exclusively exhibited low‐flow low‐gradient (LFLG) AS and less severe class of aortic valvular calcium score (5.6% vs. 60%; p = 0.006) compared to those without CTS.ConclusionOur findings suggest that CTS may delineate two phenotypes in TTRwt‐CA: a systemic phenotype associated with advanced CA and poorly calcified LFLG AS, and a cardiac phenotype characterized by less severe CA and a mixed pattern of highly calcified AS, suggesting disparate pathophysiologies.
Impact of aging on the fracture strength of 3D‐printed fixed implant prostheses: A comparative analysis of monolithic and bi‐layer with or without fiber‐reinforced composite frameworks
Authors: Moreira Cançado Renata , Sakuno Adilson , Floriani Franciele , Zandinejad Amirali , Saraceni Cintia Helena Coury , Giovani Elcio Magdalena , Mesquita Alfredo Mikail Melo 
Published: November 2024

AbstractPurposeThis study aims to evaluate the fracture strength of three‐unit, implant‐supported fixed dental prostheses (FDPs) constructed from 3D‐printed resin. It compares structures with and without fiber‐reinforced composite (FRC) frameworks under conditions that mimic artificial aging.Materials and MethodsForty FDPs were fabricated and divided into two groups: monolithic (n = 20) and bi‐layer (n = 20). The monolithic group (MG) consisted entirely of FDPs made from 3D‐printed resin for permanent restorations, while the bi‐layer group (BG) featured FDPs with an FRC framework veneered with the same 3D‐printed resin. Each group was subdivided into two subgroups: one subjected to artificial aging (MTG, n = 10; BTG, n = 10), and the other served as the baseline control (n = 10). All subgroups underwent a mechanical bending test, applying a single load to failure using a universal testing machine.ResultsThere was a statistically significant difference in fracture strength due to the presence of the FRC framework (p < 0.01). The framework became the FDP more resistant to load. The mean load to failure and standard deviations were as follows: MG 406.59N (±33.84), MTG 286.20N (±152.15), BG 1142N (±162.88), and BTG 945N (±211.52). Although no statistical differences in strength were observed between aged and nonaged subgroups, variations in failure patterns emerged, with the BG and BTG groups showing a predominance of failures compared to the MG.ConclusionsThe inclusion of an FRC framework significantly enhanced the fracture strength of the FDPs. While the aging protocol did not alter the strength, failures predominantly occurred in the 3D‐printed resin superstructure at the connectors. The highest rate of fractures occurred in the premolar connector.
N–3 Fatty Acid Supplementation in Mothers and Infants for Childhood Psychomotor and Cognitive Development: An Updated Systematic Review and Meta‐Analysis
Authors: Liu Yingyu , Zhong Lijun , Sun Zhouyang , Feng Yuan , Ding Qianlu , Zhang Yujian 
Published: November 2024

ABSTRACTLong‐chain n–3 polyunsaturated fatty acid (PUFA) consumption in maternal and infants has been positively associated with cognitive and visual development. Tails even meta‐analysis showed mixed results. To evaluate the effects of maternal and infant n–3 PUFA supplementation on childhood psychomotor and cognitive development, PubMed, Embase, the Cochrane Library, PsycINFO and clinicaltrials.gov were searched. Randomized controlled trials were included to evaluate the effect on child cognitive and psychomotor outcomes of n–3 PUFA supplementation in mothers or infants (age ≤ 2 years). Findings were pooled with mean differences (MD) with 95% confidence intervals (95% CIs). Heterogeneity was explored using I2 and subgroup analyses, stratified for maternal (pregnancy and/or lactation) and infant (preterm infant and term infant). We identified 47 articles, with 14 trials on mothers and 33 on infants. Pooled results showed that infants' mental development index (MDI) increased with n–3 PUFA supplementation (MD = 2.91, 95% CI: 1.32–4.51, I2 = 65.1%). Subgroup analysis of MDI also demonstrated a benefit in preterm infants (MD = 4.16, 95% CI: 1.40–6.93, I2 = 49.5%) and term infants (MD = 2.28, 95% CI: 0.27–4.29, I2 = 70.1%). No significant association was found in subgroup analyses of supplementation to mothers during pregnancy or lactation period. Supplementation did not increase the psychomotor development index (PDI) in the mother or infant group. Language composite score increased for infants whose mothers accepted supplementation in pregnancy or breastfeeding (MD = 8.57, 95% CI: 5.09–12.04, I2 = 70.2%). The cognitive composite score did not improve in any subgroup. Intelligence Quotient (IQ) increased in the infants' group with n–3 PUFA supplementation (MD = 2.54, 95% CI: 0.45–4.63, I2 = 66.0%). Furthermore, IQ in term infants also improved (MD = 2.91, 95% CI: 0.24–5.57, I2 = 69.2%). The funnel plot and Egger's test confirmed no publication bias in any endpoints. Supplementation with n–3 PUFA during pregnancy or breastfeeding in mothers has increased language abilities. Furthermore, direct supplementation in term infants can improve intelligence in later childhood. However, insufficient evidence supports the claim that supplementation improves cognitive abilities.
Genomic correlates of vascular plant reproductive complexity and the uniqueness of angiosperms
Authors: Leslie Andrew B. , Mander Luke 
Published: November 2024

Summary

Whole genome duplication (WGD) likely plays an important role in plant macroevolution, and has been implicated in diversification rate shifts, structural innovations, and increased disparity. But the general effects of WGD are challenging to evaluate, in part due to the difficulty of directly comparing morphological patterns across disparate clades.

We explored relationships between WGD and the evolution of reproductive complexity across vascular plants using a metric based on the number of reproductive part types. We used multiple regression models to evaluate the relative importance of inferred WGD events, genome size, and a suite of additional variables relating to growth habit and reproductive biology in explaining part type complexity.

WGD was a consistent predictor of reproductive complexity only among angiosperms. Across vascular plants generally, reproductive biology, clade identity, and the presence of bisexual strobili (those that produce microsporangiate and megasporangiate organs) were better predictors of complexity.

Angiosperms are unique among vascular plants in combining frequent polyploidy with high‐reproductive complexity. Whether WGD is mechanistically linked to floral complexity is unclear, but we suggest widespread polyploidy and increased complexity were ultimately facilitated by the evolution of herbaceous growth habits in early angiosperms.
Xizang meadow degradation alters resource exchange ratio, network complexity, and biomass allocation tradeoff of arbuscular mycorrhizal symbiosis
Authors: Dong Qiang , Ren Shijie , Willing Claire Elizabeth , Adams Catharine Allyssa , Li Yaoming , Ji Baoming , Gao Cheng 
Published: November 2024

Summary
The response of arbuscular mycorrhizal (AM) symbiosis to environmental fluctuations involves resource exchange between host plants and fungal partners, associations between different AM fungal taxa, and biomass allocation between AM fungal spore and hyphal structures; yet a systematic understanding of these responses to meadow degradation remains relatively unknown, particularly in Xizang alpine meadow.
Here, we approached this knowledge gap by labeling dual isotopes of air 13CO2 and soil 15NH4Cl, computing ecological networks of AM fungal communities, and quantifying AM fungal biomass allocation among spores, intra‐ and extraradical hyphae.
We found that the exchange ratio of photosynthate and nitrogen between plants and AM fungi increased with the increasing severity of meadow degradation, indicating greater dependence of host plants on this symbiosis for resource acquisition. Additionally, using 18S rRNA gene metabarcoding, we found that AM fungal co‐occurrence networks were more complex in more degraded meadows, supporting the stress gradient hypothesis. Meadow degradation also increased AM fungal biomass allocation toward traits associated with intra‐ and extraradical hyphae at the expense of spores.
Our findings suggest that an integrated consideration of resource exchange, ecological networks, and biomass allocation may be important for the restoration of degraded ecosystems.
Proteolysis of host DEAD‐box RNA helicase by potyviral proteases activates plant immunity
Authors: Jia Zhaoxing , Rui Penghuan , Fang Xinxin , Han Kelei , Yu Tianqi , Lu Yuwen , Zheng Hongying , Chen Jianping , Yan Fei , Wu Guanwei 
Published: November 2024

Summary

The precise mechanisms by which plant viral proteases interact with and cleave host proteins, thereby participating in virus–host interactions, are not well understood. Potyviruses, the largest group of known plant‐infecting RNA viruses, are known to rely on the nuclear inclusion protease a (NIa‐Pro) for the processing of viral polyproteins.

Here, we demonstrate that the proteolytic activity of NIa‐Pro from potyvirus turnip mosaic virus (TuMV) is indispensable for inducing hypersensitive cell death in Nicotiana benthamiana. NIa‐Pro targets and degrades the host DEAD‐box protein 5 (DBP5) via a specific cleavage motif, which initiates host cell death. Both the silencing of DBP5 and the overexpression of NIa‐Pro lead to an increased frequency of stop codon readthrough, which could be potentially harmful to the host, as it may result in the production of aberrant proteins. Unlike the NIa‐Pro of most other potyviruses, the NIa‐Pro of tobacco etch virus can also degrade DBP5 and trigger cell death, in both pepper and N. benthamiana.

Furthermore, we discovered that the TuMV‐encoded nuclear inclusion b can counteract NIa‐Pro‐induced cell death by co‐opting DBP5.

These findings unveil hitherto uncharacterized roles for plant virus proteases in cleaving host proteins and highlight the role of host DBP5 in modulating plant immunity.
Past innovations and future possibilities in plant chromosome engineering
Authors: Liu Yang , Liu Qian , Yi Congyang , Liu Chang , Shi Qinghua , Wang Mian , Han Fangpu 
Published: November 2024

SummaryPlant chromosome engineering has emerged as a pivotal tool in modern plant breeding, facilitating the transfer of desirable traits through the incorporation of alien chromosome fragments into plants. Here, we provide a comprehensive overview of the past achievements, current methodologies and future prospects of plant chromosome engineering. We begin by examining the successful integration of specific examples such as the incorporation of rye chromosome segments (e.g. the 1BL/1RS translocation), Dasypyrum villosum segments (e.g. the 6VS segment for powdery mildew resistance), Thinopyrum intermedium segments (e.g. rust resistance genes) and Thinopyrum elongatum segments (e.g. Fusarium head blight resistance genes). In addition to trait transfer, advancements in plant centromere engineering have opened new possibilities for chromosomal manipulation. This includes the development of plant minichromosomes via centromere‐mediated techniques, the generation of haploids through CENH3 gene editing, and the induction of aneuploidy using KaryoCreate. The advent of CRISPR/Cas technology has further revolutionized chromosome engineering, enabling large‐scale chromosomal rearrangements, such as inversions and translocations, as well as enabling targeted insertion of large DNA fragments and increasing genetic recombination frequency. These advancements have significantly expanded the toolkit for genetic improvement in plants, opening new horizons for the future of plant breeding.
A Ball Python Colour Morph Implicates MC1R in Melanophore–Xanthophore Distribution and Pattern Formation
Authors: Garcia‐Elfring Alan , Roffey Heather L. , Abergas Jaren M. , Wuyts Jurgen , Hendry Andrew P. , Tzika Athanasia C. , Barrett Rowan D. H. 
Published: November 2024

ABSTRACTReptiles showcase an extensive array of skin colours and patterns, yet little is known about the genetics of reptile colouration. Here, we investigate the genetic basis of the Clown colour morph found in captive‐bred ball pythons (Python regius) to study skin pigmentation and patterning in snakes. We obtained samples by crowdsourcing shed skin from commercial breeders and hobbyists. We applied a case–control design, whole‐genome pool sequencing, variant annotation, histological analyses, and electron microscopy imaging. We identified a missense mutation in a transmembrane region of the melanocortin‐1 receptor (MC1R) associated with the Clown phenotype. In classic avian and mammalian model species, MC1R is known for controlling the type and amount of melanin produced. In contrast, our results suggest that MC1R signalling might play a key role in pattern formation in ball pythons, affecting xanthophore–melanophore distribution. This work highlights the varied functions of MC1R across different vertebrate lineages and promotes a novel model system to study reptile colouration.
Inclusion Management Practices as a Pathway to Enhance Organizational Resilience in Response to a Crisis: An Empirical Test in the Context of the COVID‐19 Pandemic
Authors: Li Yixuan , Song Yifan , Wang Mo , Wu Yanbin , Zhu X. Susan , Alonso Alexander 
Published: November 2024

ABSTRACTOrganizations inevitably face various forms of disruptive events (e.g., external crises), and sustaining long‐term prosperity requires them to stay resilient when encountering unexpected adversity. Prior crisis management research predominantly relied on qualitative case studies to examine efforts after a crisis had occurred, treating the crisis as a “given” rather than a variable. The exceptionality of crisis situations and the ad hoc nature of crisis countermeasures largely limit current knowledge about how organizations may manage employees to remain in a preparative stance for disruptive events. Integrating the inclusion literature, crisis management research, and event system theory, we propose inclusion management practices as a viable pathway for organizations to develop resilience resources and capabilities prior to a crisis, allowing them to exhibit greater robustness and agility when a crisis arises. Such robustness and agility, in turn, enhance organizational performance thereafter. We further pinpoint the strength of a crisis event as an important contingency shaping the effects of pre‐crisis inclusion management practices on organizations’ resilient responses and thereby performance. We tested our hypotheses in the context of the COVID‐19 pandemic crisis using longitudinal manager‐report survey data (N = 884 workplaces). We found that workplaces that implemented more inclusion management practices before COVID‐19 were more robust and agile in response to the pandemic crisis. Agility (but not robustness), in turn, was positively related to organizational performance. In addition, the effect of inclusion management practices on agility was stronger for workplaces with greater COVID‐19 event strength.
How will we work to conduct research and revitalization with our Indigenous language and culture?
Authors: de la Cruz Abelardo 
Published: November 2024

AbstractFrom the twentieth century to today, foreigners and mestizos have conducted research on Nahua culture and the Nahuatl language from an etic perspective. In this article, I discuss how Nahuas see the role of non‐Indigenous researchers studying our language and culture and what role we—Indigenous Mexican scholars—must undertake in field research to revitalize Nahuatl, our language. I also discuss immigration from rural places to the city, which causes linguistic displacement and identity change. Finally, I propose a new way of engaging in community research through scholarship. When Nahuas carry out a research project, we make a decision to explore and learn about our culture from an emic perspective through which we engage in the language revitalization. We emphasize linguistic documentation using our mother tongue and then disseminate the Indigenous knowledge in the academia and in our small towns.