bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes
Authors: Rebelo Ana Rita , Bortolaia Valeria , Kjeldgaard Jette S , Pedersen Susanne K , Leekitcharoenphon Pimlapas , Hansen Inge M , Guerra Beatriz , Malorny Burkhard , Borowiak Maria , Hammerl Jens Andre , Battisti Antonio , Franco Alessia , Alba Patricia , Perrin-Guyomard Agnes , Granier Sophie A , De Frutos Escobar Cristina , Malhotra-Kumar Surbhi , Villa Laura , Carattoli Alessandra , Hendriksen Rene S 
Published: February 2018

Background and aim
Plasmid-mediated colistin resistance mechanisms have been identified worldwide in the past years. A multiplex polymerase chain reaction (PCR) protocol for detection of all currently known transferable colistin resistance genes (mcr-1 to mcr-5, and variants) in Enterobacteriaceae was developed for surveillance or research purposes. Methods: We designed four new primer pairs to amplify mcr-1, mcr-2, mcr-3 and mcr-4 gene products and used the originally described primers for mcr-5 to obtain a stepwise separation of ca 200 bp between amplicons. The primer pairs and amplification conditions allow for single or multiple detection of all currently described mcr genes and their variants present in Enterobacteriaceae. The protocol was validated testing 49 European Escherichia coli and Salmonella isolates of animal origin. Results: Multiplex PCR results in bovine and porcine isolates from Spain, Germany, France and Italy showed full concordance with whole genome sequence data. The method was able to detect mcr-1, mcr-3 and mcr-4 as singletons or in different combinations as they were present in the test isolates. One new mcr-4 variant, mcr-4.6**, was also identified. Conclusions: This method allows rapid identification of mcr-positive bacteria and overcomes the challenges of phenotypic detection of colistin resistance. The multiplex PCR should be particularly interesting in settings or laboratories with limited resources for performing genetic analysis as it provides information on the mechanism of colistin resistance without requiring genome sequencing.
An Embedded Fuzzy Logic Based Application for Density Traffic Control System
Authors: Adewale Ajao Lukman , Jumoke Ajao Falilat , Adegboye Mutiu , Ismail Abideen 
Published: February 2018

The control of density traffic at cross junction road usually manned by human efforts or implementation of automatic traffic light system. This system seem and proves to be inefficient with some challenges. The major constraints of this traffic control are as a result of the inability of most traffic control systems to assign appropriate waiting time for vehicles based on the lane density. Also with little or no consideration for pedestrians, emergency and security agents priorities. In view of this, an intelligent density traffic control system using (fuzzy logic) which is capable of providing priority to the road users based on the density and emergency situations was developed and presented in this paper. This system will obtain the approximate amount of vehicle and presence of pedestrians respectfully on each lane with help of Infrared Sensors (IR) and siren detection system for emergency and security road users. The working principle of this system depending on the logic inputs rules given into the processing unit by the (sensors, S1 and S2) which helps the system to generates a timing sequence that best suit the number of vehicles and pedestrians available on the lane at point in time.
Comparison between Performance of Okra Waste and Wheat Bran for Removing some Heavy Metals from Wastewater
Authors: Esgair Karim Khalifa 
Published: February 2018

In the present work, the agricultural wastes which are wheat bran and raw okra waste used as adsorbent material for adsorption of cadmium and copper ions from wastewater .The effect of adsorption variable which include initial pH of solution , agitation speed, agitation time, initial concentration of cadmium and copper ions, and amount of adsorbent material were investigated in a batch process in order to obtain the maximum ions removal from wastewater .The results obtained from experimental investigation show that the percentage removal of metal ions increases with increasing pH and agitation speed until a maximize value after that it decreased with increasing pH and agitation speed. Also increases with increasing amount of adsorbent material and agitation time until a maximize value then reach a constant value approximately , and decreasing with increasing metal ions concentration .The maximum removal percent of cadmium and copper ions were 85.8% and 52.7 % respectively which obtained at pH equal 5.0, agitation speed 150 revolution per minute, agitation time 105 minute , metal ion concentration 40mg /L ,and adsorbent amount 1.5gm when using wheat bran as adsorbent material ,while obtained the maximum removal percent of cadmium and copper ions were 81.7% and 47.8 % which obtained at pH equal 6.0, and pH equal 5.0 respectively , agitation speed 150 revolution per minute, agitation time 90 minute ,metal ion concentration 40 mg /L ,and adsorbent amount 1.5gm when using okra waste as adsorbent material. From above result the wheat bran and okra waste was a best adsorbent material for removal cadmium and copper ions from wastewater but wheat bran slightly more effective than okra waste.
PERAN PELABUHAN PERIKANAN SAMUDERA NIZAM ZACHMAN DALAM MENDUKUNG INDUSTRI TUNA (The Role of Nizam Zachman Oceanic Fishing Port to Support Tuna Industries)
Authors: Hutapea Roma Y.F , Solihin Iin , Nurani Tri Wiji 
Published: February 2018

ABSTRACTNizam Zachman Oceanic Fishing Port (OFP) has basic facilities, supportive facilities, and functional facilities that are needed for fishing activities in the port. The role of Nizam Zachman OFP as one of the largest tuna fishing ports in Indonesia is to support the availability of facilities required by each tuna industry stakeholders. The aimed of the research were to determine the role of Nizam Zachman OFP in providing support facilities and services in the activities of tuna fishing industries; and to determine the level of interest tuna stakeholders and level performance of Nizam Zachman OFP. Data analysis were carried out using a scoring method based on Ministry Regulation of Marine Affair and Fisheries number 8/PERMEN-KP/2012 about Fisheries Port then adapted to the conditions in the sites and analyze the level of interest and performance level with IPA (Importance Performance Analysis). The results showed that the role of Nizam Zachman OFP was good in production activities, infrastructure and public services, and there were still several service atributes performance that still have to be improved by the fishing port authority.Keywords: tuna industries, IPA, Nizam Zachman, fishing port role, scoring ABSTRAKPelabuhan Perikanan Samudera Nizam Zachman memiliki fasilitas pokok, fasilitas penunjang, dan fasilitas fungsional yang sangat dibutuhkan dalam setiap aktivitas perikanan di pelabuhan. Peran PPS Nizam Zachman sebagai salah satu pelabuhan perikanan tuna terbesar di Indonesia dalam menunjang ketersediaan fasilitas diperlukan oleh setiap stakeholder industri tuna. Tujuan dari penelitian ini adalah untuk menentukan peran PPS Nizam Zachman dalam menyediakan fasilitas pendukung dan pelayanan dalam kegiatan industri perikanan tuna; dan menentukan tingkat kinerja PPS Nizam Zachman serta tingkat kepentingan stakeholder tuna. Analisis data dilakukan dengan pembobotan atau scoring berdasarkan Peraturan Menteri Kelautan dan Perikanan Nomor 8/PERMEN-KP/2012 tentang Kepelabuhanan Perikanan yang kemudian disesuaikan dengan kondisi di lokasi penelitian. Selanjutnya dilakukan analisis tingkat kepentingan dan kinerja dari PPS Nizam Zachman dengan menggunakan analisis IPA (Importance Performance Analysis). Hasil penelitian menunjukkan bahwa PPS Nizam Zachman berperan baik dalam aktivitas produksi, sarana prasarana dan pelayanan umum dan terdapat beberapa atribut pelayanan PPS Nizam Zachman yang masih harus ditingkatkan kembali kinerjanya.Kata kunci: industri tuna, IPA, Nizam Zachman, peran pelabuhan perikanan, skoring
HOW DO HIGH ACHIEVER STUDENTS APPLY LEARNING STRATEGIES ON READING?
Authors: Susanti Yunik , Mulyantama Dwiki Ade 
Published: February 2018

Learning strategies used by the learners to help them comprehend, learn, or retain new information and achieve learning goals easily. This research aims at finding how the students apply learning strategies in their reading activity. The goals are to find the kinds and the most frequent learning strategies on reading together with the reason of using those most frequent strategies. This case study was carried out on the high achievers third grade students of English Department University of Nusantara PGRI Kediri. The data are collecting using questionnaire ,documentation and interview . The data then analyzed using quantitative data analysis for questionnaire data andquanitative analysis for the rest. There are three points that can be discovered from this research. 1) There are six learning strategies are used by high achievers, 2) Compensation Strategy is the strategy which is the most frequently used by high achievers in solving the problems of reading. 3) High achievers students have different reasons in applying learning strategies when they get problems, it is because of interest and urgent time.Based on three finding above, students should be aware the benefit of having learning strategies during they are learning English, especially in reading.By knowing that Compensation Strategy is the most frequent strategy used, the lecturers or teachers can use teaching strategies that appropriate with this strategy, for example: in reading exercise, students are given two passages with different contexts, but using same words or phrases in some parts of passages. So, students are able to differenciate the meaning of word when they guess it in a passage.
Learning Strategies in Speaking Skill Applied by A Student in Second Grade of SMK Pelita Nusantara Kediri
Authors: Nurhajati Diani , Azizah Alfianti 
Published: February 2018

Most of the students get difficulties when they want to communicate to others. It is caused many factors including the limited students’ vocabularies, pronunciation, the idea that they are delivered, and knowledge of grammar. To increase the students’ speaking ability, they usually apply certain strategies in learning. This research aims to investigate what strategies and how they are applied to solve the difficulties in speaking and increasing the ability of speaking.The subject of research is a student in second grade of SMK Pelita Nusantara Kediri. She is the best student of second grade and she always gets the best ranking. The writer did some observations and interview to get the data. There are two kinds of learning strategies that applied; they are direct strategies and indirect strategies. Direct strategies are language learning strategies that directly involve the target language, and indirect strategies are language learning strategies that support and manage language learning without directly involving the target language. Direct strategies that are applied are memory strategies that reflect very simple principles such as arranging things in order, making association, and reviewing, cognitive strategies that most important strategies are practicing, and compensation strategies which intended to make up for an adequate repertoire of grammar and, especially, of vocabularies. In indirect strategies that are applied are metacognitive strategies which provide a way for learners to coordinate their own learning process, affective strategies that help to regulate emotions, motivations, and attitudes, and social strategies that can help all learners increase their ability to empathize by developing culture understanding and becoming aware of others’ thoughts and feelings.It can be found that the strategies mostly used were cognitive strategies and social strategies. Cognitive strategy used was practicing by repeating, recognizing and using formula and patterns, and practicing naturalistically. Social strategies used were asking questions and cooperating with others.
PROMOTING MIND-MAPPING TECHNIQUE TO IMPROVE NURSING STUDENTS’ WRITING SKILL
Authors: Febrijanto Yoyok 
Published: February 2018

A teacher can use many ways to make students easier to study writing. Mind-mapping is a way to solve problems in writing. This technique is chosen since it has many advantages. The main benefit is that it uses both halves of the brain which makes it easier to remember. Moreover, making mind-mapping is a creative process and a natural way to organize the thoughts and it is widely believed to ease a pathway towards successful communication. Nursing students mostly do not pay attention to their writing skill. There are some indications which show the students’ poor ability in writing, such as; the students do not organize their writing well, their ideas are not coherence, they do not use right tenses in the right context, they have lack of vocabulary and their understanding of mechanic is low. They also think that time for writing is limited. Because of these, they become unmotivated and have low interest in writing. Some students even give up and do not try to solve their problem. In this paper, the writer wants to describe the use of mind-mapping technique to build up a good writing practice as an alternative teaching learning process and to activate the students’ participation in the classroom.

Keywords: Mind-Mapping Technique, Writing Skill.
THE IMPLEMENTATION OF TEACHING ENGLISH BASED ON CURRICULUM 2013 APPLIED BY AN ENGLISH TEACHER OF JUNIOR HIGH SCHOOL
Authors: Andariyani Nur , Nurhajati Diani 
Published: February 2018

Curriculum 2013 has been implemented by many schools, including a junior high school at Kediri. English teachers, who implement it, must have sufficient knowledge on what to teach, how to teach, and how to assess students’ competence, etc. The idea of implementing this curriculum is that graduates of school levels are able to compete with the graduates from other countries. Students should have communicative competence in English which covers four language skills. Therefore, this research aims at answering the question: “How is the implementation of teaching English based on Curriculum 2013 applied by an English teacher of junior high school at Kediri?” The subject of this research is an English teacher of junior high school at Kediri. It is a qualitative research which focused on the material development, teaching steps, and classroom management. The data was found from observation during teaching and learning process, interview the teacher, and documentation. The result showed that in teaching English the teacher developed the materials by modifying and extending the tasks based on the Richard and Harmer’s theory. Then, in delivering the materials, the teacher tried to implement the scientific approach, although the application was different from the concept from the scientific approach proposed by the government. Furthermore, the teacher also managed the classroom well by applying group works, and setting the comfortable condition in the class during teaching and learning process.

Key words: teaching English, Curriculum 2013, materials development, teaching steps, classroom management.
A Restitusi dan Proses Managerial Pemeriksaan Restitusi Pajak Pertambahan Nilai di KPPN Manokwari
Authors: Usman Sarah Waode 
Published: February 2018

Pajak Pertambahan Nilai (PPN) adalah suatu jenis pajak yang berbasis konsumsi yang dikenakan atas nilai tambah dari suatu barang atau jasa yang ditransaksikan. Di Indonesia pada tahun 1983 telah menerapkan pajak pejualan (PPn) dan kemudian pada 1 April 1985 diganti dengan nama Pajak Pertambahan Nilai (PPN). apabila terdapat kelebihan pembayaan pajak pertambahan nilai sebagaimana dilaporkan dalam Surat Pemberitahun (SPT) atau apabila terdapat kekeliruan pemungutan atau pemotongan yang menyebabkan kelebihan pembayaran pajak yang disebut restitusi. Hak ini dapat ditunaikan setelah mengajukan permohonan yang ditindaklanjuti dengan pemeriksaan dan penelitian akan kebenaran pembayaran pajak.
Masalah penelitian adalah bagaimana tingkat restitusi terhadap penerimaan pajak pertambahan nilai di kantor pelayanan manokwari dan bagaimana proses pemeriksaan restitusi di kantor pelayanan manokwari. Penelitian ini menggunakan mix method, yaitu kuantitaif yang menjelaskan tentang pengaruh restitusi dan penerimaan pajak pertambahan nilai, serta kualitatif yang menjelaskan proses pemeriksaan restitusi di Kantor Pelayanan Pajak Pratama Manokwari. Desain dalam penelitian ini yaitu secara Sequential Explanatory. Data yang digunakan data restitusi, penerimaan pajak pertambahan nilai 2010 – 2015 dan Proses pemeriksaan restitusi. Alat analisis adalah regresi sederhana yang menggambarkan hubungan variabel bebas (X) adalah restitusi pajak pertambahan nilai dan variabel terikat (Y) adalah penerimaan pajak pertambahan nilai. Selanjutnya melihat proses restitusi yang dilakukan dengan verifikasi yaitu melihat prosedur tatacara pemeriksaan restitusi berdasarkan peraturan dan penyelenggaraan pemeriksaan restitusi oleh kantor pelayanan pajak manokwari.
Hasil yang diperoleh secara regresi adalah Nilai koefisien determinasi (KD) yang diperoleh adalah 23% yang dapat ditafsirkan bahwa variabel bebas yaitu restitusi berkontribusi sebesar 23% terhadap variabel Y yaitu penerimaan pajak dan 77% lainnya dipengaruhi oleh faktor-faktor lain diluar variabel restitusi pajak (X). nilai korelasi adalah 0,480, dengan significant sebesar 0,336 yang berarti model regresi linier bersifat lemah dan variabel restitusi tidak mempengaruhi variabel penerimaan pajak. Proses pemeriksaan restitusi di Kantor Pelayanan Pajak Manokwari berlangsung sesuai dengan Peraturan Menteri Keuangan RI Nomor 184/PMK.03/2015 tentang Tata Cara Pemeriksaan.
Pemanfaatan Website Sebagai Media Promosi Dan Meningkatkan Peserta Didik Pada (Ma) Madrasah Aliyah Ibrohimiyyah Demak
Authors: Hanu Febryantahanuji 
Published: February 2018

Perkembangan teknologi informasi berkembang pesat terutama untuk kepentingan bisnis dan promosi sebuah produk maupun perusahaan. Tidak ketinggalan layaknya perusahaan. Begiti pula dalam dunia pendidikan, hal ini menjadi dampak yang cukup berarti di bidang pendidikan untuk terus menunjukkan eksistensinya dalam dunia teknologi. Dalam perkembangan teknologi informasi terutama internet yang dapat menimbulkan rasa ketertarikan dan penasaran karena internet dapat membuat banyak orang tidak merasa mudah jenuh, dengan internet kita dapat dengan mudah untuk membuka jendela informasi yang cepat dan terkini. Maka tidak ada salahnya beberapa instansi mulai menggunakan website. Dengan website siapapun dapat mempromosikan sebuah instansi metode tersebut secara tidak langsung berhubungan dengan teknologi informasi, salah satu caranya adalah dengan memanfaatkan website sebagai media pemasaran dan promosi. Diharapkan MA Ibrohimiyyah dapat menggunakan website untuk meningkatkan promosi instansi dan peserta didik. Dengan adanya website promosi tidak akan terbatas oleh tempat dan waktu. Sehingga hal ini diharapkan dapat meningkatkan peserta didik sesuai dengan harapan instansi.
Kata kunci: Pemanfaatan website, Pemasaran, Promosi, Media Pemasaran
IS THERE ANY IMPACT OF SOCIAL MEDIA ON ACADEMIC PERFORMANCE? (An Empirical Study From College Student In Jakarta)
Authors: Nurcahyo Bagus 
Published: February 2018

This study aimed to examine empirically the social network activities that affect academic performance. Obtain sample data using questionnaires and obtain data from top 10 universities in Webometrics and total 75 respondents. This study examined the utilization period of social networks, social network usage motivation, academic data and activities respondents perceived influence on academic performance. Using multiple regression techniques, the study found that social network activity does not negatively impact on academic performance.

Keywords: social network, Facebook, Twitter, Academic Performance, GPA
Efektivitas Minyak Atsiri Serai Wangi (Combypogon nardus) Sebagai Insektisida Alami Untuk Kecoa Amerika (Periplaneta americana)
Authors: Retno Arimurti Anindita Riesti 
Published: February 2018

ABSTRAK Cockroaches are one of the most common types of insects around our neighborhood, and it is one of the most dangerous insects because some species of cockroaches are known to transmit disease in humans such as tuberculosis, typhoid, asthma, cholera, and hepatitis. Eradication of cockroaches can be done physically and chemically, the use of chemical-based insecticides can cause respiratory problems. In an insecticide drug containing natural ingredients contain essential oils, the content of essential oil compounds serve as an anti-insect drug on citronella. This study aimed to determine the effect of essential oils of citronella fragrance to cockroaches. This type of research was experimental. The population in this study were cockroaches (Periplaneta americana) obtained from the houses of villagers of Tanjung, Sampang, Madura. The total sample was 32 adult stage cockroaches. Data analysis was using free T test with error rate of 5% or 0,05.The results showed that cockroaches stopped moving with an average of 15.75. Based on T test results showed the significant results of 0.00 which was smaller than 0.05 then H0 was rejected Ha was accepted. It can be concluded that the essential oils of citronella fragrance have an effect on the death of cockroaches. Keywords: Cockroach, Essential Oil Of Citronella Fragrance (Cymbopogon nardus)
Efektivitas Perasan Daun Keji Beling (Sericocalyx crispus Linn) Dalam Menghambat Pertumbuhan Staphylococcus aureus
Authors: Artanti Dita 
Published: February 2018

Indonesia with the title gemah ripah loh jinawi is never separated from the wealth of its natural resources, especially on the type of flora. Indonesia is rich in the types of medicinal plants one of which is a Keji beling plant. The Keji beling plants by the community are used as anti diabetic, diuretic, antisipilis, antioxidant, antimicrobial, and laksatif. A research has been conducted to determine the effectiveness of the vermicelli leaves (Sericocalyx crispus linn) in inhibiting the growth of Staphylococcus aureus. This study was experimental laboratory consisting of 24 samples with 6 treatments with 4 replications. The concentration of the vermicelli leaves is 0%, 10%, 25%, 50%, 75%, and 100%. The method used in this research is dilution method. Data were analyzed by ANOVA analysis at 5% error rate (α = 0,05). The follow-up test uses Tukey's Honest Significant Difference (Tukey's HSD) test. The results showed that the vermicelli leaves are effective in inhibiting the growth of Staphylococcus aureus. The concentration of the best Keji beling leaves that can inhibit the growth of S. aureus is at 100% concentration because only 19 colonies grow. While the minimum inhibitory power is obtained at a concentration of 75%. Key word : Keji beling leaves, Staphylococcus aureus
ANALISA DAN PERANCANGAN APLIKASI SISTEM PENDUKUNG PENGAMBILAN KEPUTUSAN PEMINATAN JURUSAN BERBASIS ANDROID DEVELOPMENT TOOLS (ADT) DENGAN PENERAPAN METODE NAIVE BAYES DAN TEOREMA BAYES
Authors: Suherdi Devri 
Published: February 2018

Penentuan peminatan jurusan ini dilakukan dengan menggunakan metode naïve baye, Naïve Bayes merupakan metode Bayesian Learning yang paling cepat dan sederhan. Penentuan peminatan dilakukan dengan menghitungpeluang terhadap mata kuliah dasar yang wajib lulus bagi mahasiswa/I Aplikasi peminatan jurusan berbasis android ini dirancang menggunakan gabungan bahasa pemograman Java SDK dan Android SDK dengan menggunakan eclips sebagai editor program yang di bunding dalam satu aplikasi yaitu Android Development Tools ( ADT ). Aplikasi ini sudah biasa digunakan untuk penentuan peminatan jurusan. Nilai KHS sebagai input dasar penentuan peminatan. Namun aplikasi ini masih perlu pengembangan lebih lanjut agar dapat di jalankan dalam jaringan atau internet sehingga dapat di akses kapan pun dan dimana Pun yang dibutuhkan oleh pengguna