bluuebunny/crossref_metadata_2025_split huggingface dataset:
IMPACT OF CYP2D6 POLYMORPHISMS IN PREDUCTING OCCURRENCE OF ADVERSE EFFECT TO TAMOXIFEN AND DEVELOPING RECCURENCE IN ER+ BREAST CANCER PATIENETS:
Authors: Boucenna Amira , Hirech Ahmed , Larbi Rezgoune Mohamed , Satta Dalila 
Published: November 2024

Objectives: Breast cancer is a major public health in Algeria. Tamoxifen has been approved in the treatment of ER+ breast cancer. Some of negative side effects of tamoxifen are frequently a reason for discontinuation of therapy during treatment, which would otherwise be potentially lifesaving. In the current study, we assessed the association between CYP2D6 polymorphisms and tamoxifen efficacy in Algerian population receiving tamoxifen as adjuvant therapy in ER+ breast cancer.
Methods: A total of 76 Algerian hormone receptor-positive premenopausal breast cancer patients with adjuvant tamoxifen treatment were investigated (45.36±6.13). DNA genotyping was performed by TaqMan Open Array technology. Tamoxifen and its metabolites levels were measured by ultra-high-performance liquid chromatography (UHPLC) followed by electro spray tandem mass spectrometry (LC-MS/MS).
Results: A significant association between the presence of deficit copy of enzyme activity and development of adverse effect after the commencement of tamoxifen therapy. Low plasma endoxifen were observed in patients categorized as (NM/PM), (IM/ IM), (IM/PM) and (PM/PM). Patients with increased plasma endoxifen concentrations were significantly more likely than patients with reduced or null activity to not report recurrences (P<0.05). We realized that the combination genotypes NM/PM, IM/IM, IM/PM, with PM/PM were more strongly associated with disease recurrence and adverse effects than NM carries of CYP2D6*1 allele (P<0.05).

Conclusion: Our results affirm that CYP2D6 polymorphism should be considered in predicting the occurrence of adverse effect fatty liver in women treated with tamoxifen. Thus, alternative treatment can be intended and lifestyle modifications can be implemented
Influence of saponin tauroside Sx1 on lifespan and expression of vitamin D receptors in the liver tissue of mice with influenza infection
Authors: Sataieva T. P. , Maligina V. Yu. , Makalish T. P. , Kriventsov M. A. , Gurtovaya A. K. 
Published: November 2024

Introduction. Respiratory infections, including influenza, are often accompanied by hepatitis in humans which pathogenesis is not fully understood. According to the available datan D deficiency is presumably a risk factor in the occurrence of acute respiratory viral infections due to its modulation of immune response. Recent studies indicate that several plant compounds can interact with vitamin D receptors (VDRs) and modulate the activity of VDRs. The biologically active components saponins have found widespread use in clinical practice due to their wide range of biological and pharmacological effects which mechanisms are still largely unclear.Aim. To study the effect of oral administration of saponin tauroside Sx1, obtained from Crimean ivy, on life expectancy and activation of vitamin D receptors in the liver of mice during experimental viral infection of varying severity.Materials and methods. The 11 subgroups were formed from male BALB/c mice, were used in the experiment depending on the infectious dose of influenza virus, 5 LD50 virus or 10 LD50 virus, respectively, including control. Saponin tauroside Sx1 was used as a corrector. Immunohistochemical studies were carried out automatically in a BOND-MAX immunohistainer (Leica, Germany). Primary rabbit polyclonal antibodies to the vitamin D receptor were used.Results and discussion. Due to the administration of tauroside Sx1 at a dose of 200 μg/mouse/day increases the average life expectancy of animals receiving saponin by 4.6 days. Reducing the infectious dose of IV from 10 LD50 to 5 LD50 also changed the onset of death of animals by 2 days in both groups. With different infectious doses of the virus, on the 10th day of the experiment in the subgroups, the expression of vitamin D receptors changes without correction. In subgroup 2V, the number of total positive cells was lower than the control group. Moreover, in the 2Vir subgroup, VDR expression was significantly higher than the control group.Conclusion. The saponin tauroside Sx1 at a dose of 200 µg/mouse/day has a fairly pronounced antiviral effect during experimental infection of mice with the influenza A/H1N1 virus, which is manifested by an increase in the average life expectancy of animals (for 4.6 days) and a decrease in the mortality rate during severe influenza infection, compared with the control group, where 100 % mortality was observed by the 14th day of the experiment. The introduction of saponin on the 4th day of the experiment in all subgroups reduces the total number of immune cells that intensively express VDR.
A valuable or a curse resource? A systematic review on expansion, perception of local community, benefits and side effects of Prosopis juliflora
Authors: Eshetu Amogne Asfaw 
Published: November 2024

IntroductionProsopis juliflora has posed a severe threat to human and animal life in arid and semi-arid areas of the world. Thus, this review intends to shed light on the potential use of Prosopis as a helpful resource and feasible management system.MethodsThis research employs a systematic review methodology.ResultsThe review revealed that the species had been introduced to overcome deforestation and desertification. Prosopis juliflora has had different socioeconomic and environmental impacts on local communities. The most widely cited adverse impacts include out-competing native species and quickly reducing forage plants, being a harbour for predators, health problems and death of domestic animals, and being an ideal ground for mosquito breeding. On the other hand, the species provides multiple ecosystem services like the provision of construction materials, animal feed and charcoal; plays a role in soil conservation and rehabilitation of degraded and saline soils, good sources of nectar for honey production; contributes to reducing dust pollution; enhances carbon sequestration, and have medicinal value due to its antifungal and antibacterial features. The local community’s attitude regarding the species is mixed.DiscussionThe consequent negative impacts are increasing quickly, making the urgent need to develop robust and practical management strategies necessary to both mitigate the adverse effects and fully use the benefits. Commercialization (with a notion of management through utilization) is a feasible way to minimize expansion. Cross-boundary endeavours for controlling Prosopis juliflora expansion to new regions, particularly in countries predicted as edges of high potential invasion, are required to manage the species invasion efficiently.
Inter-site comparability of 4D flow cardiovascular magnetic resonance measurements in healthy traveling volunteers—a multi-site and multi-magnetic field strength study
Authors: Müller Maximilian , Daud Elias , Langer Georg , Gröschel Jan , Viezzer Darian , Hadler Thomas , Jin Ning , Giese Daniel , Schmitter Sebastian , Schulz-Menger Jeanette , Trauzeddel Ralf F. 
Published: November 2024

BackgroundTime-resolved 3D cine phase-contrast cardiovascular magnetic resonance (4D flow CMR) enables the characterization of blood flow using basic and advanced hemodynamic parameters. However, different confounders, e.g., different field strength, scanner configurations, or sequences, might impact 4D flow CMR measurements. This study aimed to analyze the inter-site reproducibility of 4D flow CMR to determine the influence of said confounders.MethodsA cohort of 19 healthy traveling volunteers underwent 4D flow CMR at four different sites (Sites I–III: 3 T scanner; Site IV: 1.5 T scanner; all Siemens Healthineers, Erlangen, Germany). Two protocols of one 4D flow CMR research sequence were performed, one acquiring velocity vector fields in the thoracic aorta only and one in the entire heart and thoracic aorta combined. Basic and advanced hemodynamic parameters, i.e., forward flow volume (FFV), peak and mean velocities (Vp and Vm), and wall shear stress (3D WSS), at nine different planes across the thoracic aorta (P1–P2 ascending aorta, P3–P5 aortic arch, P6–P9 descending aorta) were analyzed. Based on a second scan at Site I, mean values and tolerance ranges (TOL) were generated for inter-site comparison. Equivalency was assumed when confidence intervals of Sites II–IV lay within such TOL. Additionally, inter- and intra-observer analysis as well as a comparison between the two protocols was performed, using an intraclass correlation coefficient (ICC).ResultsInter-site comparability showed equivalency in P1 and P2 for FFV, Vp, and Vm at all sites. Non-equivalency was present in various planes of P3–P9 and in P2 for 3D WSS in one protocol. In total, Site IV showed the most disagreements. Protocol comparison yielded excellent (&gt;0.9) ICC in every plane for FFV, good (0.75–0.9) to excellent ICC for Vm and 3D WSS, good to excellent ICC in eight planes for Vp, and moderate (0.5–0.75) ICC in one plane for Vp. Inter- and intra-observer analysis showed excellent agreement for every parameter.ConclusionsBasic and advanced hemodynamic parameters revealed equivalency at different sites and field strength in the ascending aorta, a clinically important region of interest, under a highly controlled environment.
QTc interval prolongation and risk of atrial fibrillation recurrence: a meta-analysis and observational cohort study
Authors: Ding Qiuju , Wang Zhigang , Lu Lichong , Song Zhizhao , Ge Min , Zhou Qing 
Published: November 2024

IntroductionCorrected QT interval (QTc) is a ventricular repolarization marker on electrocardiography. Previous studies evaluated its value in predicting atrial fibrillation (AF) occurrence. However, its predictive efficacy for AF recurrence remains controversial.MethodsWe searched PubMed and Google databases for studies before January 2024 evaluating the association between QTc interval and AF incidence. A meta-analysis of the eligible datasets was conducted using Bazett's formula, with subgroup analysis to explore the heterogeneity. Additionally, thirty-eight patients with AF who underwent radiofrequency catheter ablation were enrolled and followed-up for 3–36 months. Univariate and multivariate Cox models were used to calculate the hazard ratios (HRs) and determine the relationship between clinical factors and AF recurrence. Kaplan-Meier survival analysis and ROC curve were conducted to assess the impact and predictive efficacy of individual factors.ResultsEleven datasets from nine eligible studies were enrolled and meta-analysed. We found that patients with prolonged QTc interval was associated with a significantly higher AF incidence risk, and the risk increased with every 10-ms prolongation. However, this association was not significant in the AF recurrence subgroup. In our prospective cohort, the preoperative body mass index, QTc, left atrial diameter (LAD), and uric acid levels influenced AF recurrence. Multivariate Cox regression analysis identified LAD as an independent factor affecting AF recurrence in patients with a high predictive efficiency. Kaplan-Meier survival analysis showed that increased LAD (&gt;4.5 cm) was associated with postoperative AF recurrence.DiscussionTherefore, LAD has better predictive power and can be an indicator for predicting postoperative AF recurrence.
Analysis of risk factors for autoimmune thyroid disease based on blood indicators and urinary iodine concentrations
Authors: Liu Jianning , Feng Zhuoying , Gao Ru , Liu Peng , Meng Fangang , Fan Lijun , Liu Lixiang , Du Yang 
Published: November 2024

ObjectiveThe aim of this study was to elucidate the relationships between thyroid hormones, lifestyle factors, biochemical markers, and autoimmune thyroid disease (AITD), thereby identifying the factors influencing the development of these diseases.MethodsThe study encompassed 517 patients with AITD and 549 patients with non-autoimmune thyroid disease. Demographic and clinical data were collected, and various laboratory indicators, including urinary iodine and thyroid hormones, were measured and compared between the groups. Lasso regression was employed to select the independent variables, while logistic regression analysis determined the factors associated with the development of AITD.ResultsThe prevalence of drinking alcohol history, median urinary iodine, and TSH concentrations proved significantly greater in the AITD group compared to the control group, while FT3 levels demonstrated lower values within the AITD group (p&lt;0.05). Furthermore, there was a significant difference in the distribution of iodine nutrition status between the two groups (p&lt;0.05). Both univariate and multivariate logistic regression analyses revealed significant associations among excessive iodine intake, drinking alcohol history, TSH, FT3, and the development of AITD.ConclusionsExcessive iodine intake and drinking alcohol history are implicated in an augmented risk of developing AITD. The prevention of AITD may necessitate the regular monitoring of TSH and FT3 concentrations.
The multifaceted nature of diabetic erectile dysfunction: uncovering the intricate mechanisms and treatment strategies
Authors: Ma Jianxiong , Chen Yihao , Si Yuhe , Qian Jiahua , Wang Chenxi , Jin Juan , He Qiang 
Published: November 2024

BackgroundOne of the most common complications of diabetes mellitus is diabetic erectile dysfunction (DMED), a condition that has grown more common in recent years and has a significant impact on patients’ daily lives. The complicated pathophysiological changes of DMED, involving vascular, neurological, muscular, and endocrine variables, have not been well addressed by any one treatment technique, and no widely approved treatment strategy has been developed.AimThe objective of this study was to thoroughly examine the complex nature of the pathogenic mechanism of DMED and discover new therapeutic approaches that could improve DMED symptoms.MethodsStudies and review articles from the past 10 years were considered.ResultsThe pathogenesis of DMED encompasses vascular dysfunction, endothelial cell damage, cavernous smooth muscle defects, neurological dysfunction, endocrine/metabolic factors, leukomalacia fibrosis, and psychosocial factors, elucidating complex interplay among the mechanisms underlying DMED. It underscores the need of integrating traditional herbal medicine, energy-based medicine treatments, and advanced techniques like stem cell and gene therapy to enhance therapeutic outcomes. Furthermore, it expresses optimism on the therapeutic potential of new nanobiomaterials in DMED.ConclusionThrough integrating a complete description of DMED etiology and current therapy methods, this work offers a helpful resource for researchers, doctors, and patients dealing with this difficult condition.
Awareness, use, motivations and methods of accessing genetic testing in 2022 in the United States
Authors: Makhnoon Sukh , Lee MinJae , Prasad Tanushree , Badalamenti Alexa , Gurley Tami , Waters Erika A. , Skinner Celette Sugg 
Published: November 2024

IntroductionAwareness, access, and use of clinical and direct-to-consumer (DTC) genetic tests has increased in recent years with documented disparities in these services. We provide updated data on test awareness and use, and report novel data on motivations and methods for accessing genetic tests.MethodsNationally representative data from the 2022 Health Information National Trends Survey (HINTS 6) were used to assess awareness and use of ancestry, personal trait, specific disease, and carrier testing by sociodemographic characteristics, examine reasons for undergoing tests, and methods of accessing them.ResultsOverall, 81.4% of respondents were aware and 40.0% had undergone testing. Only 10% of tests were ordered by genetic counselors, 80% of carrier and 65% of specific disease tests were ordered by other healthcare providers. Understanding family history was the most common reason for undergoing ancestry (72.2%) or personal trait tests (64.9%) whereas reasons such as doctor’s recommendation (53%–59%), learning more about disease risk (18%–50%), and carrier testing (76%) were common for undergoing disease risk tests and carrier tests. In contrast to ancestry, personal trait, and carrier testing, there were no racial, ethnic, income, or rural/urban difference in use of specific disease risk testing.DiscussionDiffusion of genetic tests into US society, although incremental, has made sizable increases in awareness, equitable use of specific disease tests but worsening socioeconomic inequality in DTC genetic test use. The study provides update on the state of genetic testing in the US and identifies groups that may need help accessing clinical genomic information and services.
Immune-based subgroups uncover diverse tumor immunogenicity and implications for prognosis and precision therapy in acute myeloid leukemia
Authors: Chen Tingting , Zhang Yue , Zhang Danyang , Zhou Hebing 
Published: November 2024

BackgroundAlthough a considerable proportion of acute myeloid leukemia (AML) patients achieve remission through chemotherapy, relapse remains a recurring and significant event leading to treatment failure. This study aims to investigate the immune landscape in AML and its potential implications for prognosis and chemo-/immune-therapy.MethodsIntegrated analyses based on multiple sequencing datasets of AML were performed. Various algorithms estimated immune infiltration in AML samples. A subgroup prediction model was developed, and comprehensive bioinformatics and machine learning algorithms were applied to compare immune-based subgroups in relation to clinical features, mutational landscapes, immune characterizations, drug sensitivities, and cellular hierarchies at the single-cell level.ResultsTwo immune-based AML subgroups, G1 and G2, were identified. G1 demonstrated higher immune infiltration, a more monocytic phenotype, increased proportions of monocytes/macrophages, and higher FLT3, DNMT3A, and NPM1 mutation frequencies. It was associated with a poorer prognosis, lower proportions of various immune cell types and a lower T cell infiltration score (TIS). AML T-cell-based immunotherapy target antigens, including CLEC12A, Folate receptor β, IL1RAP and TIM3, showed higher expression levels in G1, while CD117, CD244, CD96, WT and TERT exhibited higher expression levels in G2. G1 samples demonstrated higher sensitivity to elesclomol and panobinostat but increased resistance to venetoclax compared to G2 samples. Moreover, we observed a positive correlation between sample immune infiltration and sample resistance to elesclomol and panobinostat, whereas a negative correlation was found with venetoclax resistance.ConclusionOur study enriches the current AML risk stratification and provides guidance for precision medicine in AML.
Effects of replacing fish meal with pork meal and supplementing with crystalline amino acids and fish oil on growth performance, body composition, enzyme activity, intestinal morphology, and immune response of Penaeus monodon
Authors: Chen Qiuchi , Zhang Chunxiao , Yun Biao , Wang Sheng , Ma Jun , Qian Xueqiao 
Published: November 2024

IntroductionThe present study aimed to evaluate the effects of substituting fish meal with pork meal in feed on the growth performance, feed utilization, intestinal morphology, and immune function of Penaeus monodon.MethodsA total of 600 uniformly sized juvenile shrimp with an initial weight of 1.20 ± 0.03 g were randomly assigned to four groups, with each group consisting of six replicates of 25 shrimp each. Fish meal protein was replaced with 0%, 5%, 10%, and 15% pork meal, and crystalline amino acids and fish oil were supplemented accordingly. The groups were designated as P0, P5, P10, and P15, and the study duration was 70 days.ResultsResults indicated no significant difference in specific growth rate (SGR) between the P5 and P10 groups compared to the P0 group. However, the SGR in the P15 group was significantly lower than that in the P5 and P10 groups. Feed conversion rates (FCRs) for the P5 and P10 groups were comparable to the P0 group, whereas the P15 group showed a significantly higher FCR than the other groups. There were no significant differences in whole body composition among all the groups. Apart from alanine, the amino acid composition of muscles did not differ significantly among all the groups. Results of enzyme activity in plasma revealed that the replacement of fish meal with pork meal reduced levels of aspartate aminotransferase (AST) and superoxide dismutase (SOD). Specifically, levels of AST in the P5 and P15 groups were significantly lower compared to the P0 and P10 groups. Histological examination of midgut tissues showed that villus height initially increased and then decreased with higher replacement ratios. The P5 group had the highest villus height, while the P15 group had the lowest villus height. Analysis of gene expression related to liver immune functions indicated that genes in TLR signaling pathway were significantly elevated in the P15 group compared to the other groups.DiscussionIn conclusion, even with the supplementation of crystalline amino acids and fish oil, pork meal should not replace more than 10% of fish meal in the feed of P. monodon to maintain optimal growth and feed efficiency.
The correlation between non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (NHHR) with non-alcoholic fatty liver disease: an analysis of the population-based NHANES (2017–2018)
Authors: Yang Yuhao , Li Shengxi , An Zhenmei , Li Shuangqing 
Published: November 2024

Background/objectiveNon-alcoholic fatty liver disease (NAFLD) encompasses a spectrum of liver disorders, from benign steatosis to more severe conditions like non-alcoholic steatohepatitis, with risks of progressing to fibrosis, cirrhosis, and hepatocellular carcinoma. The non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (NHHR) indicates lipid metabolic dysregulation and is associated with increased risks of various diseases. This study examines the relationship between NHHR and NAFLD to evaluate NHHR as a potential predictive biomarker for NAFLD.MethodsData from the 2017–2018 National Health and Nutrition Examination Survey (NHANES) were used for cross-sectional analysis. After excluding individuals with incomplete data, hepatitis infections, heavy alcohol use, and those under 18, the study included 2,757 adults. The relationship between NHHR and NAFLD was analyzed using multivariable logistic regression, including subgroup analysis and interaction testing.ResultsAmong the 2,757 participants (mean age 49.91 years), 44.9% had NAFLD. NHHR showed a significant positive association with NAFLD, with an unadjusted odds ratio (OR) of 1.71 and a fully adjusted OR of 1.45. Quartile analysis revealed a 228% higher prevalence of NAFLD in the highest NHHR quartile, with an OR of 3.28. This positive association was consistent across various subgroups.ConclusionOur findings suggest that elevated NHHR is positively correlated with the prevalence of NAFLD and possesses predictive value. We recommend that future research validate the clinical utility of NHHR, particularly for early detection of high-risk individuals and guiding personalized interventions.