bluuebunny/crossref_metadata_2025_split huggingface dataset:
The transcription factor FcWRKY40 of Fortunella crassifolia functions positively in salt tolerance through modulation of ion homeostasis and proline biosynthesis by directly regulating SOS2 and P5CS1 homologs
Authors: Dai Wenshan , Wang Min , Gong Xiaoqing , Liu Ji‐Hong 
Published: May 2018

Summary

Although some WRKYs have been characterized, regulatory roles of most WRKYs remain poorly understood.

Herein, we elucidated function of FcWRKY40 from Fortunella crassifolia in salt tolerance via overexpression and virus‐induced gene silencing (VIGS) and unraveled its target genes.

Overexpression of FcWRKY40 enhanced salt tolerance in transgenic tobacco and lemon, while silencing of FcWRKY40 increased salt susceptibility. Homolog genes of Salt Overly Sensitive 2 (SOS2) and Δ‐1‐pyrroline‐5‐carboxylate synthetase 1 (P5CS1) were dramatically up‐regulated in transgenic lemon but down‐regulated in VIGS line. Consistently, transgenic lemon displayed lower Na+ and higher proline concentrations, whereas the silenced line accumulated more Na+ but less proline. Treatment of transgenic lemon with 24‐epi‐brassinolide compromised salt tolerance, while supply of exogenous proline partially restored salt tolerance of the VIGS line. FcWRKY40 specifically binds to and activates promoters of FcSOS2 and FcP5CS1. FcWRKY40 was up‐regulated by ABA and salt, and confirmed as a target of ABA‐responsive element binding factor 2 (FcABF2). Moreover, salt treatment up‐regulated FcABF2 and FcP5CS1, and elevated proline concentrations.

Taken together, our findings demonstrate that FcWRKY40 participates in the ABA signaling pathway and as a positive regulator functions in salt tolerance by regulating genes involved in ion homeostasis and proline biosynthesis.
Precision medicine for multiple sclerosis promotes preventative medicine
Authors: Hansen Madison R. , Okuda Darin T. 
Published: May 2018

AbstractMultiple sclerosis (MS) is a chronic, lifelong disease, currently without a cure that is responsible for significant neurological injury in young adults. Precision medicine for MS aims to provide a more exacting and refined approach toward management by providing recommendations based on disease subtype, clinical status, existing radiological data, para‐clinical data, and other biological markers. To achieve better outcomes, the three stages of care—diagnosis, treatment, and management—should be optimized. However, as the temporal profile of disease behavior is highly variable in MS, and unlike outcomes from other chronic conditions (i.e., hypertension, diabetes mellitus, etc.), should precision medicine for MS be one that focuses more on disease prevention and lifestyle modifications beyond recommendations for the use of disease‐modifying therapies? As scientific advancements continue within the field of neuroimmunology, and until reliable biomarkers that predict disease outcomes are available, success may be better achieved by focusing on modifiable factors to reduce future disability.
Choosing Wisely in pediatric anesthesia: An interpretation from the German Scientific Working Group of Paediatric Anaesthesia (WAKKA)
Authors: Becke Karin , Eich Christoph , Höhne Claudia , Jöhr Martin , Machotta Andreas , Schreiber Markus , Sümpelmann Robert 
Published: May 2018

SummaryInspired by the Choosing Wisely initiative, a group of pediatric anesthesiologists representing the German Working Group on Paediatric Anaesthesia (WAKKA) coined and agreed upon 10 concise positive (“dos”) or negative (“don'ts”) evidence‐based recommendations. (i) In infants and children with robust indications for surgical, interventional, or diagnostic procedures, anesthesia or sedation should not be avoided or delayed due to the potential neurotoxicity associated with the exposure to anesthetics. (ii) In children without relevant preexisting illnesses (ie, ASA status I/II) who are scheduled for elective minor or medium‐risk surgical procedures, no routine blood tests should be performed. (iii) Parental presence during the induction of anesthesia should be an option for children whenever possible. (iv) Perioperative fasting should be safe and child‐friendly with shorter real fasting times and more liberal postoperative drinking and enteral feeding. (v) Perioperative fluid therapy should be safe and effective with physiologically composed balanced electrolyte solutions to maintain a normal extracellular fluid volume; addition of 1%‐2.5% glucose to avoid lipolysis, hypoglycemia, and hyperglycemia, and colloids as needed to maintain a normal blood volume. (vi) To achieve safe and successful airway management, the locally accepted airway algorithm and continued teaching and training of basic and alternative techniques of ventilation and endotracheal intubation are required. (vii) Ultrasound and imaging systems (eg, transillumination) should be available for achieving central venous access and challenging peripheral venous and arterial access. (viii) Perioperative disturbances of the patient's homeostasis, such as hypotension, hypocapnia, hypothermia, hypoglycemia, hyponatremia, and severe anemia, should not be ignored and should be prevented or treated immediately. (ix) Pediatric patients with an elevated perioperative risk, eg, preterm and term neonates, infants, and critically ill children, should be treated at institutions where all caregivers have sufficient expertise and continuous clinical exposure to such patients. (x) A strategy for preventing postoperative vomiting, emergence delirium, and acute pain should be a part of every anesthetic procedure.
An actin‐depolymerizing factor from the halophyte smooth cordgrass, Spartina alterniflora (SaADF2), is superior to its rice homolog (OsADF2) in conferring drought and salt tolerance when constitutively overexpressed in rice
Authors: Sengupta Sonali , Mangu Venkata , Sanchez Luis , Bedre Renesh , Joshi Rohit , Rajasekaran Kanniah , Baisakh Niranjan 
Published: May 2018

SummaryActin‐depolymerizing factors (ADFs) maintain the cellular actin network dynamics by regulating severing and disassembly of actin filaments in response to environmental cues. An ADF isolated from a monocot halophyte, Spartina alterniflora (SaADF2), imparted significantly higher level of drought and salinity tolerance when expressed in rice than its rice homologue OsADF2. SaADF2 differs from OsADF2 by a few amino acid residues, including a substitution in the regulatory phosphorylation site serine‐6, which accounted for its weak interaction with OsCDPK6 (calcium‐dependent protein kinase), thus resulting in an increased efficacy of SaADF2 and enhanced cellular actin dynamics. SaADF2 overexpression preserved the actin filament organization better in rice protoplasts under desiccation stress. The predicted tertiary structure of SaADF2 showed a longer F‐loop than OsADF2 that could have contributed to higher actin‐binding affinity and rapid F‐actin depolymerization in vitro by SaADF2. Rice transgenics constitutively overexpressing SaADF2 (SaADF2‐OE) showed better growth, relative water content, and photosynthetic and agronomic yield under drought conditions than wild‐type (WT) and OsADF2 overexpressers (OsADF2‐OE). SaADF2‐OE preserved intact grana structure after prolonged drought stress, whereas WT and OsADF2‐OE presented highly damaged and disorganized grana stacking. The possible role of ADF2 in transactivation was hypothesized from the comparative transcriptome analyses, which showed significant differential expression of stress‐related genes including interacting partners of ADF2 in overexpressers. Identification of a complex, differential interactome decorating or regulating stress‐modulated cytoskeleton driven by ADF isoforms will lead us to key pathways that could be potential target for genome engineering to improve abiotic stress tolerance in agricultural crops.
Midazolam as a first‐line treatment for neonatal seizures: Retrospective study
Authors: Dao Kim , Giannoni Eric , Diezi Manuel , Roulet‐Perez Eliane , Lebon Sebastien 
Published: May 2018

AbstractMidazolam is commonly used to treat refractory seizures in newborns and as a first‐line anti‐epileptic drug in children. Its use as first‐line treatment of neonatal seizures has not been investigated so far. We retrospectively studied the tolerability of midazolam in 72 newborn infants who received i.v. or i.n. midazolam as first‐line treatment for seizures. No major side‐effect exclusively due to midazolam was reported. The i.n. route was used for 20 patients (27.8%). Effectiveness could not be formally evaluated due to the absence of systematic electroencephalogram recording while midazolam was administered. In conclusion, midazolam was well‐tolerated as a first‐line abortive emergency treatment of neonatal seizure. The i.n. route offers a useful alternative to i.v. phenobarbital or phenytoin in emergency settings.
Japanese clinical practice guidelines for allied disorders of Hirschsprung's disease, 2017
Authors: Muto Mitsuru , Matsufuji Hiroshi , Taguchi Tomoaki , Tomomasa Takeshi , Nio Masaki , Tamai Hiroshi , Tamura Masanori , Sago Haruhiko , Toki Akira , Nosaka Shunsuke , Kuroda Tatsuo , Yoshida Masahiro , Nakajima Atsushi , Kobayashi Hiroyuki , Sou Hideki , Masumoto Kouji , Watanabe Yoshio , Kanamori Yutaka , Hamada Yoshinori , Yamataka Atsuyuki , Shimojima Naoki , Kubota Akio , Ushijima Kosuke , Haruma Ken , Fukudo Shin , Araki Yuko , Kudo Takahiro , Obata Satoshi , Sumita Wataru , Watanabe Toshihiko , Fukahori Suguru , Fujii Yoshimitsu , Yamada Yoshiyuki , Jimbo Keisuke , Kawai Fujimi , Fukuoka Tomoya , Onuma Shinsuke , Morizane Toshio , Ieiri Satoshi , Esumi Genshiro , Jimbo Takahiro , Yamasaki Tomoko 
Published: May 2018

AbstractBackgroundDespite the presence of ganglion cells in the rectum, some patients have symptoms similar to those of Hirschsprung's disease. A consensus has yet to be established regarding the terminology for these diseases. We defined this group of diseases as “allied disorders of Hirschsprung's disease” and compiled these guidelines to facilitate accurate clinician diagnosis and provide appropriate treatment strategies for each disease.MethodsThese guidelines were developed using the methodologies in the Medical Information Network Distribution System (MINDS). Of seven allied disorders, isolated hypoganglionosis; megacystis‐microcolon‐intestinal hypoperistalsis syndrome; and chronic idiopathic intestinal pseudo‐obstruction were selected as targets of clinical questions (CQ). In a comprehensive search of the Japanese‐ and English‐language articles in PubMed and Ichu‐Shi Web, 836 pieces of evidence related to the CQ were extracted from 288 articles; these pieces of evidence were summarized in an evidence table.ResultsWe herein outline the newly established Japanese clinical practice guidelines for allied disorders of Hirschsprung's disease. Given that the target diseases are rare and intractable, most evidence was drawn from case reports and case series. In the CQ, the diagnosis, medication, nutritional support, surgical therapy, and prognosis for each disease are given. We emphasize the importance of full‐thickness intestinal biopsy specimens for the histopathological evaluation of enteric ganglia. Considering the practicality of the guidelines, the recommendations for each CQ were created with protracted discussions among specialists.ConclusionsClinical practice recommendations for allied disorders of Hirschprung's disease are given for each CQ, along with an assessment of the current evidence. We hope that the information will be helpful in daily practice and future studies.
Comparison of basiliximab vs antithymocyte globulin for induction in pediatric heart transplant recipients: An analysis of the International Society for Heart and Lung Transplantation database
Authors: Butts Ryan J. , Dipchand Anne I. , Sutcliffe David , Bano Maria , Dimas Vivian , Morrow Robert , Das Bibhuti , Kirk Richard 
Published: May 2018

AbstractThis study aims to compare 2 common induction strategies, basiliximab and ATG. Analysis of the ISHLT transplant registry was performed. The database was queried for pediatric heart transplants from January 1, 2000, to June 30, 2015, who had received induction with basiliximab or ATG. Primary end‐point was graft survival. Secondary end‐points included 1‐year survival and 1‐year conditional survival. There were 3158 heart transplants who received induction with basiliximab or ATG. The ATG cohort was younger, more likely to have congenital heart disease or be a retransplant, have a higher PRA, longer ischemic time, and been transplanted earlier in the study period (all P<.01). There was no difference in graft loss in the basiliximab cohort compared to the ATG cohort (HR 1.18 P=.06). On conditional 1‐year survival analysis, basiliximab induction was associated with graft loss (HR=1.35 95% CI 1.1‐1.7, P<.01), and in the propensity‐matched cohort, the basiliximab cohort was more likely to experience rejection prior to discharge (P=.04). Infection prior to discharge was more common in the antithymocyte cohort. Induction with ATG is associated with improved late graft survival compared to basiliximab.
A case for community health programs for sickle cell anemia in low‐ to middle‐income countries: An integrative review
Authors: Ibemere Stephanie 
Published: May 2018

AbstractObjectiveThe purpose of this review is to determine the factors associated with the outcomes of community health programs with particular focus on low‐ to middle‐income countries. In addition, this review aims to suggest how these factors can affect the creation of a Sickle Cell Anemia health program in sub‐Saharan Africa.Design and SampleA literature review spanning the last decade (March 2007 to February 2017) was conducted using the Whittemore and Knafl integrative review framework.ResultsCommunity ownership, networking, and resource allocation were identified as themes that affect community health program outcomes. Attempts to use a guiding principle or process to implement community health programs were also apparent in the literature.ConclusionMore rigorous scientific investigation is needed to ensure that these community health programs are effective and have measurable outcomes. Nursing is well positioned, considering the metaparadigm concepts that guide practice, to forge this research. Nursing scientists could fill the gap in knowledge related to community health program outcomes in low‐ to middle‐income countries while achieving global objectives focused on decreasing noncommunicable disease burden.
From Prikaz to Procedures: Becoming an International Organization in Post‐Soviet Kazakhstan
Authors: Woodard Lauren 
Published: May 2018

AbstractThis article explores the collision of two bureaucratic systems at a local nongovernmental organization in Kazakhstan. In response to budget cuts by Western donors, NGO employees decided to make it an “international” organization. At first glance, being international echoes neoliberal notions of the worker and workplace that clash with the current prikaz (decree) system, a Soviet legacy and Kazakhstani bureaucratic order. Through close ethnographic analysis, I ask not only what neoliberalism means in this context but also what its appeal is to local actors. Although these two systems seem at odds with each other, they are more fluid than they first appear. I argue that being international is a process of neoliberalization in which the staff draw on their experiences with, and the language of, their international donors to adapt to an unstable funding climate, while navigating the demands of Kazakhstani bureaucracy.
“Don't Get Arrested!” Trust, Miscommunication, and Repression at the 2008 Anti‐G8 Mobilization in Japan
Authors: Maeckelbergh Marianne 
Published: May 2018

AbstractTransnational coordination and communication have become increasingly important themes in scholarship on social movements. The alterglobalization movement is one of the most globally networked movements in recent history. As part of its repertoire, every year thousands of people travel from around the world to protest the G8, the gathering of the world's eight most powerful leaders. When the G8 came to Japan in 2008, local activists decided to organize a mobilization similar to those previously held in Western Europe and North America. The shift from Europe to Japan, however, proved more difficult than anticipated. I explore three factors that together hindered the mobilization: trust, miscommunication, and state repression. Through an analysis of action planning meetings, I explore how interpersonal trust combined with dynamics of individual and collective risk to shape relations of inclusion, exclusion, and hierarchy. I describe the interplay among trust, miscommunication, and repression to show how interpersonal trust undermined the movement's own goal of prefiguring more horizontal political structures and, paradoxically, expanded the impact of state repression by creating an individuation of responsibility that implicated movement actors themselves in narrowing the forms of protest available.
European Funds and the Hermeneutics of the Capitalist Crisis: Insights from within the Greek State
Authors: Gkintidis Dimitrios 
Published: May 2018

AbstractIn this article, I seek to explore the political and epistemological implications of European Union developmental funds in settings of policymaking in Greece. Building on fieldwork with specialized technocrats in Athens and with political personnel in Evros, I initially posit that the discourse on European funds has functioned as a recurrent argument for the justification of a coherent liberal idealist paradigm starting in the 1980s. Notwithstanding the ideological usage of European funds, I suggest that its increasing relevance in the practices and worldview of these social agents also pertains to a broader relocation of political and analytical focus onto the ethical conduct and qualitative features of dominated classes. Most evidently, the recent manifestation of the capitalist crisis in Greece reactualized the precept of moral and cultural introspection as well as its interrelation with the discourse of past benefaction. Ιn this sense, the recent crisis also made evident the pertinence of Michel Foucault's “hermeneutics of the self” to understand the delineation of class hegemony in capitalism. I argue that the discourse of European funds has been conducive to the consolidation of the “hermeneutics of the self” as an overarching political imperative in Greek society over the last decades.
Caradura: Migration, Informal Labor, and the Problem of Enacting Trust in a Spanish Enclave in Morocco
Authors: Campbell Brian 
Published: May 2018

AbstractSome social scientists have argued that state legal institutions are not effective at protecting immigrants. Proper evaluation of ethnographic data, they argue, shows that migrants integrate themselves by forming strong, personal relationships of trust and friendship minimally grounded in discourses of ethnicity or kinship. They also transcend legal and cultural categories and assign roles other than those of the state. This article tests this model against the experience of migrants, refugees, and transfronterizos/as (cross‐border laborers) engaged in informal ties of confianza (trust) in Ceuta, a small Spanish enclave in Morocco. Workers claim that while ties of confianza can transform into kinship, they are also ridden with suspicion and vulnerable to exploitation. Therefore, migrants claim they must show a caradura (literally, a hard face) and be ready to stand up to their employers to protect their interests. Ceutan employers, by contrast, use confianza to manage the risks of dealing with hostile foreigners, to regulate state interference, and to integrate the migrant on their terms. Building on anthropological theories of informal and domestic work, this articles suggests that Ceutan confianza is better explained as an ambiguous, ethnically informed discourse both migrants and employers use to navigate Ceuta's unregulated shadow economy.
Museum specimens provide phylogenomic data to resolve relationships of sack‐bearer moths (Lepidoptera,Mimallonoidea,Mimallonidae)
Authors: St Laurent Ryan A. , Hamilton Chris A. , Kawahara Akito Y. 
Published: May 2018

AbstractMimallonidae, the sack‐bearer moths, are a family of predominantly Neotropical moths containing nearly 300 described species. Mimallonidae feed on over 40 host plant families and are found in a variety of environments, but phylogenetic relationships of species within the family have never been investigated. We sequenced 515 loci using anchored hybrid enrichment target capture on ethanol‐preserved and dried museum specimens, with dates of collection ranging from 1985 to 2017. We sampled 47 species, representing 32 of the 36 described mimallonid genera. By incorporating 19 dry museum specimens, and recovering an average of over 400 loci for each, we illustrate the utility of natural history collections in anchored hybrid enrichment‐based phylogenomics. Maximum likelihood and multi‐species coalescent analyses provide robust support for the recognition of six higher‐level groups within Mimallonidae, which we designate as subfamilies: Zaphantinae St Laurent & Kawaharasubfam.n., Aurorianinae St Laurent & Kawaharasubfam.n., Mimalloninae Burmeister, Lacosominae Dyar, Druenticinae St Laurent & Kawaharasubfam.n.and Cicinninae Schausstat.n.Our phylogenetic results also robustly support eight new tribes: Lacosominae: Trogopterini St Laurent & Kawaharatribe n., Lacosomini Dyarstat.n., Alheitini St Laurent & Kawaharatribe n.; Druenticinae: Luramini St Laurent & Kawaharatribe n., Druenticini St Laurent & Kawaharatribe n.; Cicinninae: Bedosiini St Laurent & Kawaharatribe n., Psychocampini St Laurent & Kawaharatribe n., Cicinnini Schausstat.n.Three new genera are also described based on our phylogenetic results:HerbinallaSt Laurent & Kawahara,gen.n.,UlalumaSt Laurent & Kawahara,gen.n.,BedosialloSt Laurent & Kawahara,gen.n.NanitetaFranclemont,syn.n.is a synonym ofLacosomaGrote. Six genera are paraphyletic, and in total 19 new combinations are proposed:Macessoga laxacomb.n.,Lacosoma elassacomb.n.,Thaelia anysiacomb.n.,Thaelia subrubiginosacomb.n.,Herbinalla caudinacomb.n.,Druentica brosicacomb.n.,Ulaluma valvacomb.n.,Cicinnus eminenscomb.n.,Roelmana pluridiscatacomb.n.,Roelmana laguerreicomb.n.,Psychocampa joannacomb.n.,Psychocampa unalcacomb.n.,Psychocampahamatacomb.n.,Psychocampa maronacomb.n.,Bedosiallo eugeniacomb.n.,Bedosiallo forbesicomb.n.,Bedosiallo moenguscomb.n.,Bedosiallo styxcomb.n.andBedosiallo sylviacomb.n.This study is the first to implement the LEP1 probe set on a comprehensive taxonomic dataset that includes many museum specimens, and our results demonstrate that museum specimens can be used in anchored hybrid enrichment studies. Importantly, these data produce a robust phylogeny that will serve as a foundation for future studies on mimallonid evolution, such as host plant relationships and biogeography.This published work has been registered in ZooBank:http://zoobank.org/urn:lsid:zoobank.org:pub:60890688‐2E77‐4B98‐B247‐B7A5F7E4DFD9.