bluuebunny/crossref_metadata_2025_split huggingface dataset:
GAMBARAN KUALITAS TIDUR PADA PENDERITA HIPERTENSI QUALITY OF SLEEP AMONG HYPERTENSION PATIENTS
Authors: Sakinah Putwi Rizki , Kosasih Cecep Eli , Sari Eka Afrima 
Published: January 2019

AbstractPeople with hypertension experience symptoms of dizziness, headache, obstructive sleep apnea, shortness of breath, nocturia, and restless legs syndrome which disturb sleep and reduce its quality. This research’s objective was to describe the sleep quality overview of Puskesmas Rancaekek’s hypertension patients. Using descriptive quantitative method with purposive sampling technique, this research aimed at 79 Puskesmas Rancaekek’s patients who had been diagnosed with hypertension for at least one month long. The data were collected using the Pittsburgh Sleep Quality Index (PSQI) questionnaire and analysed using the total score resulted; ≤5 was good while >5 was poor. Results showed 94.6% of respondents had poor sleep quality. The dimensions that contribute to the poor sleep quality included >30 minutes sleep latency, <5 hours sleep duration, <65% of sleep efficiency, sleep disturbance due to waking up at midnight or early morning and waking up to toilet, and activity disturbance at daylight. The conclusion was that Puskesmas need to develop preventive and promotive efforts to minimize the complication of hypertension patients and optimize their quality of life by helping them improve their quality of sleep. Meanwhile, the self-management that should be done by the patients were avoiding the consumption of coffee and cigarettes, limiting physical activities, and doing physical exercise and relaxation therapies. Key Words: Hypertension, Sleep Quality, PSQI AbstrakPenderita hipertensi mengalami gejala pusing, nyeri kepala, obstructive sleep apnea, sesak napas, nokturia, restless legs syndrome yang mengganggu tidur dan berdampak pada kualitas tidur serta mempengaruhi kualitas hidup dan tekanan darah. Penelitian bertujuan untuk melihat gambaran kualitas tidur pada penderita hipertensi di Puskesmas Rancaekek. Metode penelitian menggunakan deskriptif kuantitatif dengan teknik purposive sampling sebanyak 79 penderita hipertensi di Puskesmas Rancaekek yang telah didiagnosis dokter minimal 1 bulan. Pengambilan data menggunakan kuesioner Pittsburgh Sleep Quality Index (PSQI) dan dianalisis menggunakan total skor, jika skor ≤ 5 baik dan skor > 5 buruk. Hasil menunjukan 94,6% responden memiliki kualitas tidur buruk. Dimensi yang berkontribusi terhadap kualitas tidur buruk yaitu latensi tidur tidak dapat tertidur dalam waktu 30 menit, durasi tidur <5 jam, efisiensi kebiasaan tidur <65%, gangguan tidur karena terbangun tengah malam atau pagi sekali dan terbangun karena ingin ke toilet, serta gangguan aktivitas pada siang hari. Puskesmas perlu mengembangkan upaya preventif dan promotif untuk meminimalisir komplikasi penderita hipertensi dan pencapaian kualitas hidup optimal salah satunya dengan membantu meningkatkan kualitas tidur. Dengan mengedukasi penderita untuk menghindari konsumsi kopi dan rokok, membatasi aktivitas fisik, melakukan latihan fisik, dan terapi relaksasi. Kata Kunci: Hipertensi, Kualitas Tidur, PSQI
HUBUNGAN DERAJAT PENYAKIT HIPERTENSI DENGAN TINGKAT KECEMASAN PADA KELOMPOK LANJUT USIA DI WILAYAH KERJA PUSKESMAS KAHAKITANG KECAMATAN TATOARENG
Authors: Lumi Femmy , Terok Maria , Budiman Freyjer 
Published: January 2019

Hypertension is a disease that is often experienced by the elderly and it becomes a global health issue that requires special attention because it can lead to major deaths in developed countries as well as developing countries. One of the risk factors of hypertension is anxiety. Anxiety is caused due to various circumstances such as worry, anxiety, fear, discomfort accompanied by various physical complaints and health problems. The purpose of this study was to analyze the relationship of degree of hypertension with anxiety level in elderly groupThe type of this research is analytical study with cross sectional, that have 95 population elderly and become 67 elder total sampel of characteristic inclusion. Data were collected using questionnaire and analyzed by using chi-square test. The Results Discussion have Degrees of hypertensive disease in elderly individuals (36%) were at mild and severe levels with moderate anxiety level in elderly (44.78%). The result of chi-square test using SPSS, P = 0,000 which is smaller than α = 0,05 meaning that there is a very significant relation on degree of hypertension with anxiety level in elderly groupBased on the results of research, the nurse will improve the elderly health by maintaining the promotion effort in the form of health counseling to the elderly group, empowering the elderly group and maintaining the psychological condition.
UJI AKTIVITAS SEDIAAN KRIM EKSTRAK ETANOL HERBA SELEDRI (Apium graveolens L.) TERHADAP LUKA SAYAT PADA KELINCI (Oryctolagus cuniculus L.)
Authors: Djajanti Agust Dwi , Asfi Dzul 
Published: January 2019

ABSTRACT Research has been carried out on the activity test of preparations for the ethanol extract of celery herbs (Apium graveolens L.) on vegetable wounds on rabbits (Oryctolagus cuniculus L). The aim of this study was to determine the activity of cream preparations for celery herb extract using concentrations of 2% b / v, 4% b / v and negative controls. The activity test was carried out on 2 rabbit test animals by carrying out the same 3 incisions on each rabbit's back with a length of 2 cm, 0.3 cm depth and 1 incision wound for negative control. The observation was carried out to see the pharmacological effects of the cream preparation, namely anti-inflammatory effect, proliferation, and its remodeling as an anti-inflammatory effect is swollen or red, while the proliferation effect is the formation of stopped granulation tissue after the entire surface is covered in the epithelium and the process of wound maturation, and while the remodeling effect is the phase that ends when the inflammation sign is gone. Keywords: Activity, Ethanol Extract, Herbs Celery (Apium graveolens L.), Cut wounds, Rabbits (Oryctolagus cuniculus L.) ABSTRAK Telah dilakukan penelitian tentang Uji Aktivitas Sediaan Krim Ekstrak Etanol Herba Seledri (Apium graveolens L.) Terhadap Luka Sayat Pada Kelinci (Oryctolagus cuniculus L). Penelitian ini bertujuan untuk mengetahui aktivitas sediaan krim ekstrak herba seledri dengan menggunakan konsentrasi 2% b/v, 4% b/v dan kontrol negatif. Uji aktivitas dilakukan pada 2 hewan uji kelinci dengan melakukan 3 luka sayat yang sama disetiap punggung kelinci dengan panjang 2 cm, kedalaman 0,3 cm dan 1 luka sayatan untuk kontrol negatif pengamatan dilakukan untuk melihat efek farmakologi dari sediaan krim tersebut yaitu efek anti inflamasi, proliferasi, dan remodelingnya yang dimaksud efek anti inflamasi adalah bengkak atau merah, sedangkan efek proliferasi adalah pembentukan jaringan granulasi berhenti setelah seluruh permukaan tertutup epitel dan proses pendewasaan luka, dan sedangkan efek remodelingnya adalah fase yang berakhir bila tanda radang sudah hilang. Kata kunci : Aktivitas, Ekstrak etanol, Herba Seledri (Apium graveolens L.), Luka sayat, Kelinci (Oryctolagus cuniculus L.)
SITUASI PNEUMONIA DI WILAYAH KERJA DINAS KESEHATAN KABUPATEN GOWA SULAWESI SELATAN TAHUN 2017
Authors: Wibowo Wibowo , Putranto Rudi Hendro , Widianto Widianto , Sahir Sahir 
Published: January 2019

Infeksi Pneumonia muncul karena adanya invasi mikroorganisme atau virus, bakteri atau jamur pada paru-paru.Umumnya pneumonia ditandai dengan batuk-batuk berdahak dengan sputum kehijauan atau kuning, demam tinggi dan menggigil serta nafas yang pendek. Tujuan penelitian ini adalah diperolehnya gambaran kasus pneumonia di wilayah kerja Dinas Kesehatan Kabupaten Gowa. Hasil penelitian ditemukan bahwa penemuan kasus pneumonia tertinggi pada balita di wilayah Kabupaten Gowa adalah sebesar 57,7% terjadi pada balita laki-laki. Sedangkan sebesar 42,3% ditemukan pada balita perempuan. Berdasarkan hasil penelitian dapat diketahui bahwa penemuan kasus pneumonia tertinggi pada balita di wilayah Kabupaten Gowa adalah sebesar 27,5% yang terdapat di Kecamatan Sombaopu. Untuk penemuan kasus terendah pada balita di wilayah Kabupaten Gowa adalah sebesar 0,1% yang terdapat di Kecamatan Bontonompo Selatan. Ditemukan bahwa penemuan kasus pneumonia tertinggi pada balita di wilayah Kabupaten Gowa adalah sebesar 28,3% yang terdapat di Puskesmas Sombaopu. Sedangkan penemuan kasus terendah pada balita di wilayah Kabupaten Gowa adalah sebesar 0,1% yang terdapat di Puskesmas Bontonompo I Kesimpulan masih ditemukan kasus pneumonia dengan jumlah kasus dibawah angka nasional yaitu 2%.
The relationship between the umbilical cord length and it's diameter with the total CD34+ and total nucleated cell (TNC) as a parameter of cord blood selection
Authors: Indriani Meiliza , Rahadiyanto Ya’kub , Effendi Yusuf , Putera Bayu Winata , Hafy Zen 
Published: January 2019

Background: The stem cell transplantation successful influenced by the quality of the umbilical cord blood which includes the number of CD34 + and Total Nucleated Cells (TNC).
Objectives: The study aims to determine the correlation between umbilical cord length and it’s diameter, with the number of CD34 + and TNC cells as indicators of the quality of cord blood storage feasibility. Several other factors as maternal age, gestation period, and infant birth weight also examined.
Methods: Thirty four of umbilical cords from the delivered woman in Dr. Mohammad Hoesin Hospital (RSMH) Palembang were collected from May to June 2018. The length and diameter of the cord were immediately measured after delivery. The evaluated cells were counted with a flow cytometer at Klaster Stem Cell and Tissue Engineering Research Centre (SCTE) IMERI Faculty of Medicine University of Indonesia (FKUI).
Results: Spearman correlation test show that there was no correlation between the length and diameter of the umbilical cord, maternal age, gestational period and infant weight, with the number of CD34 + and TNC cells in the cord (p> 0.05). However, the profile analysis indicated that the longer and larger the diameter of the umbilical cord, the higher concentration of the CD34+ and TNC cells.
Conclusion: This study suggested that the younger maternal age, older gestational age, and higher infant birth weight, also normal hemoglobin level, tend to increase the number of CD34+ and TNC cells in the cord blood.
Enhancing cognitive function of healthy Wistar rats with aqueous extract of Centella asiatica
Authors: Rosdah Ayeshah A , Lusiana Evi , Reagan Muhammad , Akib Abdurrahman , Khairunnisa Fadhila , Husna Afkara 
Published: January 2019

Background: Centella asiatica (L.) Urb is a native herb from Asian countries such as India, China, and Indonesia. This herb has been widely used as a cure for various diseases. However, studies investigating the aqueous extract of Centella asiatica as a nootropic in healthy individuals are still very limited.
Objective: This study aims to investigate the potential of aqueous extract of Centella asiatica in enhancing cognitive function of healthy male Wistar rats.
Methods: Rats were randomly allocated to four treatment groups, i.e. without treatment and aqueous Centella asiatica extract at doses of 200, 400 and 800 mg/kg. To determine enhancement of cognitive function, novel object recognition (NOR) test was conducted after the course of treatment. Acetylcholine content was assessed by enzyme-linked immunosorbent assay.
Results: There was a significantly high preference index towards the novel object in the NOR test in groups treated with 200 mg/kg and 800 mg/kg of the aqueous extract compared to control. This was further confirmed by a significant increase of brain acetylcholine content in rats treated with 200 mg/kg of the extract.
Conclusion: Therefore, this study confirms that the aqueous extract is effective in enhancing cognitive performance of healthy Wistar rats.
The effect of intermittent hypobaric hypoxia on oxidative stress status and antioxidant enzymes activity in rat brain
Authors: Dewi Syarifah , Mulyawan Wawan , Wanandi Septelia Inawati , Sadikin Mohamad 
Published: January 2019

Background: High altitude can cause hypobaric hypoxia (HH), resulted from the lower barometric pressure and hence partial pressure of oxygen. Hypoxia can lead to a lot of deleterious molecular and cellular changes, such as generation of free radicals or reactive oxygen species (ROS). Increasing of ROS can cause oxidative stress if the antioxidant enzyme does not increase simultaneously. Oxidative damage in brain has toxic effect on cognitive functions.
Objective: In this study, we investigate effect of acute intermittent HH on oxidative stress and antioxidant enzyme activity in rat brain.
Method: Wistar rats divided into 5 groups, consisting control group and four experimental groups which treated to HH. Rats were exposed to simulated HH equivalent to 35.000 feet in hypobaric chamber for 1 minute, repeated once a week.
Results: Level of malondialdehyde and carbonyl in rat brain under acute HH increased at HH exposure (group I) compare to control group. These levels decreased afterward at intermittent HH exposure (group II-IV). Specific activity of superoxide dismutase (SOD) shows increasing level at intermittent HH exposure, especially group IV was increasing of SOD level significantly. The increasing pattern of specific activity of catalase was inversely from SOD pattern, but it still has higher activity in intermittent HH compare to control group.
Conclusion: Brain tissue seems to be able to perform an adequate adaptive response to hypobaric hypoxia after the training, shown by its significantly decreased MDA and carbonyl level and also increased specific activity of SOD and catalase.
Human serum folate can be measured using folate binding protein linked to enzyme-labeled protein ligand binding assay (ELPLBA) as well as ELISA
Authors: Budiman Muhamad Arif , Sadikin Mohamad , Prijanti Ani Retno 
Published: January 2019

Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzyme-linked immunosorbent assay) method. Folate binding protein is a protein that can bind folate, therefore it considered can be used as a tool that can replace antibody dependent ELISA method.
Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk.
Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine’s milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide.
Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363.
Conclusion: ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA).
Expression of apelin is related to oxidative damage in heart tissue of rats during chronic systemic hypoxia
Authors: Helmi H R , Ferdinal Frans , Prijanti Ani Retno , Jusman Sri Widia A , Suyatna Frans D 
Published: January 2019

Background: Chronic systemic hypoxia is severe environmental stress for the heart and might lead to the development of heart failure. Apelin is an endogenous peptide that has been shown to have various beneficial effects on cardiac function. Apelin appears to have a role to play in the ventricular dysfunction and maintaining the performance of the heart.
Objectives: In the present study we want to investigate the adaptive response of heart tissue to chronic systemic hypoxia and the correlation with apelin expression and oxidative stress in rat.
Methods: An experimental study was performed using 28 Sprague-Dawley male rats, 8 weeks of age. Rats were divided into 7 groups 4 each, namely control group; normoxia (O2 atmosphere) and the treatment group of hypoxia (8% O2) for 6 hours; 1;3;5;7 and 14 days respectively. Body weight and heart weight were measured at each treatment. Ventricular thickness was measured by caliper, Apelin mRNA was measured using real-time qRT-PCR with Livak formula and malondialdehyde (MDA) level was used to assess oxidative stress due to cardiac tissue hypoxia.
Results: Macroscopic exams showed hypertrophy at day 7th. The relative expression of Apelin mRNA in hypoxic heart is decreased at the beginning and then increased, starting from day-7 to day-14. The MDA levels were significantly increased from day-7 and were strongly correlated with relative expression Apelin.
Conclusion: It is concluded that the increase of Apelin expression is related to oxidative stress in heart tissue of rats during chronic systemic hypoxia.
Upaya Meningkatkan Kebugaran Jasmani Melalui Sirkuit Training Kids pada Siswa
Authors: Julianto Idi 
Published: January 2019

Kebugaran jasmani adalah kemampuan seseorang untuk melaksanakan tugas sehari-hari tanpa mengalamani kelelahan yang berarti dan masih memiliki cadangan tenaga untuk kegiatan yang lain. Latihan yang dapat dilakukan untuk memperoleh kebugaran jasmani sangat banyak, salah satunya adalah menggunakan sirkuit training kids. Bentuk latihan sirkuit training kids adalah model modifikasi latihan daya tahan yang disesuaikan taraf perkembangan kardiovascullar, neuromuskular, psikis dan sosial siswa. Tujuan penelitian ini adalah untuk mengetahui pengaruh sirkuit training kids terhadap kebugaran jasmani pada siswa kelas VIII SMP Negeri 4 Ciawigebang. Karena dalam penelitian ini penulis ingin mengetahui pengaruh suatu perlakuan secara langsung terhadap sampel penelitian, maka metode yang digunakan dalam penelitian ini adalah metode class action research.Sampel dalam penelitian ini adalah siswa kelas VIII-A SMP Negeri 4 Ciawigebangkabupaten Kuningan sebanyak 30 siswa. Dari analisis tes kebugaran jasmani melalui latihan sirkuit training kids tingkat keberhasilan mencapai 75,33%, artinya tingkat kebugaran siswa mengalami peningkatan sebesar 20,16% dari siklus I (64,17%). Hasil evaluasi siswa pada siklus II mengalami peningkatan dibandingkan dengan hasil evaluasi pada siklus I, dan hasil evaluasi pada siklus II mengalami peningkatan dibandingkan pada siklus I bahwa dari 30 siswa yang dinyatakan tuntas mencapai 25 siswa atau 88,33% dan sisanya yaitu 5 siswa atau 5,67% dinyatakan tingkat kebugaran kurang. Pada siklus II siswa yang dinyatakan tuntas sudah melebihi target ketuntasan belajar yang ditetapkan yaitu 75%. Hasil rata-rata tes kebugaran siklus II sebesar 74,33, yang juga mengalami peningkatan skor sebesar 10,16 dari siklus I (64,17 %).
Quantitative Analysis of Gene Expression During Calcium Homeostasis in E. coli
Authors: Arif Muhammad , Tisa Louis S 
Published: January 2019

Despite a wealth of knowledge regarding calcium’s role as secondary messenger in eukaryotic cells, relatively little is known about calcium homeostasis in bacteria. Our study employing molecular genetics and transcriptome analysis of calcium homeostasis previously exhibited a calcium regulation of gene expression in elevated or depleted calcium throughout E. coli genome. Here we report the results of our subsequent investigation into quantitative analysis using quantitative reverse transcriptase PCR (qRT-PCR) of gene expression in subsets of genes from wild-type and mutant strains with addition of calcium and chelation of calcium. Data from qRT-PCR reveal very high upregulation of genes yjeE and secA indicating their possible role as overactive calcium efflux systems in E. coli. Calcium is highly likely to be involved in stress response as evidenced by upregulation of marB in transposon mutants and by 10-fold induction of rpoS in elevated calcium condition in mutants defective in calcium transport.
Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 47-54
Antibacterial Properties of Essential Oil (EO) Extracted from Nigella sativa Linn. (Black cumin) and its Application Against Vibrio cholerae in Ground Chicken Meat
Authors: Deloer Sharmina , Bari ML , Hoque Md Mahfuzul 
Published: January 2019

Chemical preservatives for food preservation at high concentration are hazardous for public health. The natural antimicrobials from plants, herbs and spices have been given priority as alternatives of the chemical preservatives. In this study, antibacterial activity of the essential oil (3%) from black cumin seeds was tested against five food borne pathogens viz Staphylococcus aureus (ATCC 25923), Shigella dysenteriae-I (MJ-84), Escherichia coli 0157:H7 (ATCC 12079), Escherichia coli (ATCC 25922) and Vibrio cholerae( ATCC 6395) using disc diffusion method. The essential oil (EO) showed antibacterial activity against both Gram positive and Gram negative bacteria with the highest inhibition against S. aureus (17.4 ± 0.2) and the lowest against Vibrio cholerae (14.5 ± 1.0). No activity was observed against E. coli and E. coli O157:H7. The effect of temperature and pH on the antibacterial activity of the EO from black cumin seeds was determined. At 100°C for 30 min treatment, no significant reduction of antibacterial activity of the EO was observed, suggesting that high temperature does not affect the activity. The optimum temperature for the activity was recorded at 50°C. The EO showed the highest activity at pH 7.0 (optimum) with an insignificant change of activity at pH 5.0, 9.0 and 10.0. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EO against S. aureus, V. cholerae and Shigella dysenterae were determined by two fold dilution method at optimum temperature of 50°C and 37oC and optimum pH at 7.0 and 5.0, respectively, which ranged from 0.125 (S. aureus) to 1.0% (Shigella dysenterae), with the MBC of 0.25 to 2.0%, respectively. Three times of MIC value of EO (1.5%) was applied in ground chicken meat seeded with V. cholerae ATCC 6395 and was kept at -20°C for 10 days. The test organism was diminished within 24 hours, suggesting that the EO from black cumin is highly active in vivo and could be used as preservative in ground chicken meat. The results of this study provide new dimension of research on EOs from spices against foodborne pathogens in vivo in controlling their growth in meat based food and food products.
Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 55-60