bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activity of two hyaluronan preparations on primary human oral fibroblasts
Authors: Asparuhova Maria B. , Kiryak Deniz , Eliezer Meizi , Mihov Deyan , Sculean Anton 
Published: September 2018

Background and ObjectiveThe potential benefit of using hyaluronan (HA) in reconstructive periodontal surgery is still a matter of debate. The aim of the present study was to evaluate the effects of two HA formulations on human oral fibroblasts involved in soft tissue wound healing/regeneration.Material and MethodsMetabolic, proliferative and migratory abilities of primary human palatal and gingival fibroblasts were examined upon HA treatment. To uncover the mechanisms whereby HA influences cellular behavior, wound healing‐related gene expression and activation of signaling kinases were analyzed by qRT‐PCR and immunoblotting, respectively.ResultsThe investigated HA formulations maintained the viability of oral fibroblasts and increased their proliferative and migratory abilities. They enhanced expression of genes encoding type III collagen and transforming growth factor‐β3, characteristic of scarless wound healing. The HAs upregulated the expression of genes encoding pro‐proliferative, pro‐migratory, and pro‐inflammatory factors, with only a moderate effect on the latter in gingival fibroblasts. In palatal but not gingival fibroblasts, an indirect effect of HA on the expression of matrix metalloproteinases 2 and 3 was detected, potentially exerted through induction of pro‐inflammatory cytokines. Finally, our data pointed on Akt, Erk1/2 and p38 as the signaling molecules whereby the HAs exert their effects on oral fibroblasts.ConclusionBoth investigated HA formulations are biocompatible and enhance the proliferative, migratory and wound healing properties of cell types involved in soft tissue wound healing following regenerative periodontal surgery. Our data further suggest that in gingival tissues, the HAs are not likely to impair the healing process by prolonging inflammation or causing excessive MMP expression at the repair site.
Two pKM101‐encoded proteins, the pilus‐tip protein TraC and Pep, assemble on the Escherichia coli cell surface as adhesins required for efficient conjugative DNA transfer
Authors: González‐Rivera Christian , Khara Pratick , Awad Dominik , Patel Roosheel , Li Yang Grace , Bogisch Maxim , Christie Peter J. 
Published: September 2018

SummaryMobile genetic elements (MGEs) encode type IV secretion systems (T4SSs) known as conjugation machines for their transmission between bacterial cells. Conjugation machines are composed of an envelope‐spanning translocation channel, and those functioning in Gram‐negative species additionally elaborate an extracellular pilus to initiate donor‐recipient cell contacts. We report that pKM101, a self‐transmissible MGE functioning in the Enterobacteriaceae, has evolved a second target cell attachment mechanism. Two pKM101‐encoded proteins, the pilus‐tip adhesin TraC and a protein termed Pep, are exported to the cell surface where they interact and also form higher order complexes appearing as distinct foci or patches around the cell envelope. Surface‐displayed TraC and Pep are required for an efficient conjugative transfer, ‘extracellular complementation’ potentially involving intercellular protein transfer, and activation of a Pseudomonas aeruginosa type VI secretion system. Both proteins are also required for bacteriophage PRD1 infection. TraC and Pep are exported across the outer membrane by a mechanism potentially involving the β‐barrel assembly machinery. The pKM101 T4SS, thus, deploys alternative routing pathways for the delivery of TraC to the pilus tip or both TraC and Pep to the cell surface. We propose that T4SS‐encoded, pilus‐independent attachment mechanisms maximize the probability of MGE propagation and might be widespread among this translocation superfamily.
Secularization of immigration policy vs. religion's influence on integration: Israel's non‐Jewish Jews' immigration in a comparative perspective
Authors: Fisher Netanel 
Published: September 2018

AbstractThis article deals with the complex relationship between religion and immigration in Western countries, with an emphasis on Israel. The main argument it presents is that the legal procedures of immigration, i.e. laws relating to the acquisition of civil status, have undergone dramatic secularization, while religion's influence is expressed in the social and cultural aspects of the integration of immigrants belonging to religious minorities. This division reinforces the classical theory of secularization, as the formal boundaries of nations are not subject to religious affiliations, but it also supports the theories of competition and complementation between religion and secularism in the social sphere. The tension in the Israeli case between the immigration, naturalization and integration of non‐Jewish Jews, who are part of the extended Jewish population that is not defined by religious parameters, confirms this thesis. The immigration of hundreds of thousands of non‐Jewish Jews' under the Law of Return based on ethno‐national‐secular parameters is an ultimate expression of the secularization of Jewish nationality. On the other hand, the state's encouragement of non‐Jewish immigrants to convert to Judaism so that they can better assimilate into Jewish society signifies the importance of religion in the social integration aspect.
Recognition Theory in Nurse/Patient Relationships: The contribution of Gillian Rose
Authors: Cummings Rachel 
Published: September 2018

AbstractRecognition theory attempts to conceptualize interpersonal relationships and their normative political implications. British social philosopher Gillian Rose developed her own version of recognition rooted in the work of Georg Hegel. This article applies Rose's theory of recognition to care, arguing that its emphasis on lack of identity, the dynamic process of recognition and the existential risks involved accurately describes the relationship between nurse and patient. Rose's version is compared to both contemporary notions of the interpersonal in healthcare literature, other forms of recognition theory and Emmanuel Levinas's vision of intersubjective relationships, a traditional philosophical basis for nursing care. In each case, I will argue that Rosean recognition is a fuller, more complex and ultimately more satisfying view of the relationships involved.
Catheter ablation for atrial fibrillation and intervention for pulmonary vein stenosis in a patient with Fontan circulation
Authors: Uhm Jae‐Sun , Choi Jae Young , Kim Young Jin , Kim Tae‐Hoon , Joung Boyoung , Pak Hui‐Nam , Lee Moon‐Hyoung 
Published: September 2018

AbstractIncidence of atrial fibrillation (AF) is high in patients with congenial heart disease. However, management of AF is challenging in these patients. Although radiofrequency catheter ablation (RFCA) is effective therapeutic option for AF, RFCA for AF is not common in patients who underwent Fontan operation. We present a 24‐year‐old woman with paroxysmal AF, who underwent lateral tunnel Fontan operation for functional single ventricle. Circumferential pulmonary vein isolation was successfully performed. However, significant pulmonary vein stenosis developed after RFCA. Pulmonary vein stenosis was successfully treated by transcatheter intervention.
Continence surgery at the time of pelvic organ prolapse repair: a review of the literature
Authors: Loganathan Jemina , Fayyad Abdalla , Abdel‐Fattah Mohamed 
Published: September 2018

Key content

Pelvic organ prolapse and incontinence are significant health problems; one in nine women are affected by prolapse and one in three by stress urinary incontinence.

Pelvic floor muscle training remains the first‐line treatment for stress urinary incontinence.

Concomitant surgical correction for coexisting symptomatic prolapse and incontinence is an option for treatment, but persistent postoperative incontinence can be challenging to manage.

Delayed or interval continence procedures may be beneficial and should be discussed with women preoperatively.

Learning objectives

To understand the health burden and prevalence of pelvic organ prolapse and incontinence in the population.

To understand the surgical treatment options available.

To learn about the advantages and disadvantages of concomitant surgery in patients with symptomatic or occult stress incontinence and in those who are continent.

Ethical issues

Should transvaginal prolapse repair and concomitant synthetic miduretheral sling be offered to women without stress urinary incontinence?

Should all women be preoperatively screened for occult stress urinary incontinence, given its prevalence and implications for postoperative outcomes?
Genetic and genomic resources of sorghum to connect genotype with phenotype in contrasting environments
Authors: Boyles Richard E. , Brenton Zachary W. , Kresovich Stephen 
Published: September 2018

SummaryWith the recent development of genomic resources and high‐throughput phenotyping platforms, the 21st century is primed for major breakthroughs in the discovery, understanding and utilization of plant genetic variation. Significant advances in agriculture remain at the forefront to increase crop production and quality to satisfy the global food demand in a changing climate all while reducing the environmental impacts of the world's food production. Sorghum, a resilient C4 grain and grass important for food and energy production, is being extensively dissected genetically and phenomically to help connect the relationship between genetic and phenotypic variation. Unlike genetically modified crops such as corn or soybean, sorghum improvement has relied heavily on public research; thus, many of the genetic resources serve a dual purpose for both academic and commercial pursuits. Genetic and genomic resources not only provide the foundation to identify and understand the genes underlying variation, but also serve as novel sources of genetic and phenotypic diversity in plant breeding programs. To better disseminate the collective information of this community, we discuss: (i) the genomic resources of sorghum that are at the disposal of the research community; (ii) the suite of sorghum traits as potential targets for increasing productivity in contrasting environments; and (iii) the prospective approaches and technologies that will help to dissect the genotype–phenotype relationship as well as those that will apply foundational knowledge for sorghum improvement.
An early increase of CD56bright natural killer subset as dominant effect and predictor of response to extracorporeal photopheresis for graft‐versus‐host disease
Authors: Iniesta Pastora , Revilla Nuria , Chen‐Liang Tzu Hua , Hurtado Ana María , Vicente Vicente , Heras Inmaculada , Jerez Andrés , Lozano María Luisa 
Published: September 2018

BACKGROUNDCD56bright natural killer (NK) regulatory cells were recently shown to display a differential impact on the risk of developing extensive chronic graft‐versus‐host disease (GVHD). To date no study has definitively established which immune populations are most responsible for the immunomodulatory effects or response to extracorporeal photopheresis (ECP) for GVHD.STUDY DESIGN AND METHODSTo test the role of CD56bright NK cells in ECP, a prospective enhanced flow cytometry follow‐up of immune subsets (CD19+, CD3+, CD3+/CD4+, CD3+/CD8+, CD3‐/CD56+, CD3‐/CD56bright, and CD3‐/CD56dim) was performed in 32 patients with GVHD who underwent 552 procedures.RESULTSAn early increase of CD56bright NK cells was found as a hallmark effect to ECP, particularly during the first 3 months of treatment. This was also supported by the ability to predict for complete responses when this increase was expressed as a higher CD56bright versus CD56dim NK cells ratio. Among the immune subsets tested, the only variable that had direct influence on response to ECP was a CD56bright/dim ratio more than 0.16 (hazard ratio [HR] 4.32, p = 0.014; HR 5.8, p = 0.007, at 2 and 3 months of ECP treatment, respectively).CONCLUSIONThese findings argue for exploring strategies for priming a CD56bright NK cell expansion during ECP and providing additional and potentially relevant data for revisiting the underpinning cellular mechanisms of ECP that could generate that expansion.
Genomics of Salmonella contaminating backyard production systems reveals persistence and transmission of genetically related Salmonella on a farm basis
Authors: Toro Magaly , Rivera Dácil , Toledo Viviana , Campos‐Vargas Reinaldo , Allard Marc W. , Hamilton‐West Christopher , Moreno‐Switt Andrea I. 
Published: September 2018

AbstractAnimals raised in backyard productive systems (BPS) have been frequently associated with Salmonella outbreaks. Several serovars have caused these events, showing that different BPSs can be contaminated by distinct Salmonella serovars. The aim of this study was to characterize the genomic diversity of Salmonella isolates obtained from BPSs in Central Chile to understand their genomic relatedness. A whole‐genome SNP‐based phylogenetic analysis of 22 Salmonella isolates from 12 locations revealed that S. Typhimurium isolates clustered based on the BPS that they were originally isolated from, and the same was established for S. Enteritidis isolates. Furthermore, our genomic analysis shows that animals from different species (i.e., a chicken, a duck and a pig) carried genetically related S. Typhimurium strains within the same BPS. Moreover, some of these genetically related isolates were obtained in different years (2013 and 2014), indicating that farm‐specific Salmonella can persist in BPSs for multiple years and that interspecies transmission is plausible in this environment. Understanding the dynamics of interspecies transmission of Salmonella serovars within a contaminated BPS is fundamental to the design of mitigation strategies to reduce outbreaks of human Salmonella associated with backyard production systems.
Reviewer Acknowledgements
Authors: Smith Robert 
Published: September 2018

Journal of Education and Training Studies (JETS) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether JETS publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue. Reviewers for Volume 6, Number 10Ahmet Hakan Hancer, TurkeyAhmet Turan Orhan, TurkeyAngelina Sánchez, Martí, Universitat Autònoma de Barcelona, SpainBerfin Serdil Örs, Adnan Menderes University, TurkeyBurcu GÜVENDİ, TurkeyCagla Atmaca, Pamukkale University, TurkeyCarmen Pérez-Sabater, Universitat Poltècnica de València, SpainEbru Çetin,Gazi University, TurkeyEbru Kilic Cakmak, TurkeyEnisa Mede, Bahcesehir University, TurkeyFroilan D. Mobo, Philippine Merchant Marine Academy, PhilippineHanifi Üzüm, Abant İzzet Baysal Universty, TurkeyHulya Yumru, Istanbul Aydın University, Turkeyİbrahim Yaşar Kazu, Firat University, Turkeyİlknur Özal Göncü, Gazi University, TurkeyIntakhab Khan, King Abdulaziz University, Saudi ArabiaJohn Bosco Azigwe, Bolgatanga Polytechnic, GhanaJohn Cowan, Edinburgh Napier University, UKJohn Jongho Park, University of Michigan, USAJon S. Turner, Missouri State University, USAJulide Inozu, Adana Cukurova University, TurkeyKadir Yıldız, Manisa Celal Bayar Universty, TurkeyKadir Yıldız, TurkeyKatya De Giovanni, University of Malta, MaltaLaura Bruno, The College of New Jersey, USALinda J. Rappel, Yorkville University/University of Calgary, CanadaMarcie Zaharee, The MITRE Corporation, USAMehmet Inan, Marmara University, TurkeyMeral Seker, Alanya Alaaddin Keykubat University, TurkeyMert Aydoğmuş, Karabuk University, TurkeyMutar Taş, TurkeyNevzat Demirci, Mersin University, TurkeyÖzkan Işık, Sakarya University, TurkeyPhil Sirinides, University of Pennsylvania, USASandro Sehic, Oneida BOCES, USAŞengül Atasoy, Recep Tayyip Erdoğan Universty, TurkeySimona Savelli, Università degli Studi Guglielmo Marconi, ItalySüleyman Gönülateş, Pamukkale University, TurkeyVeronica Rosa, University Rome, ItalyYalçın Dilekli, Aksaray University, Turkey Robert SmithEditorial AssistantOn behalf of,The Editorial Board of Journal of Education and Training StudiesRedfame Publishing9450 SW Gemini Dr. #99416Beaverton, OR 97008, USAURL: http://jets.redfame.com
Creating allegiance: leading transformational change within the NHS
Authors: Tweed Alison , Singfield Andrew , Taylor Julia R A , Gilbert Lucy , Mount Paul 
Published: September 2018

BackgroundWithin the UK National Health Service (NHS) the move to Sustainable Transformation Plans/Partnerships and Integrated Care Systems reflect the increasing need and expectation for transformational change at a system level across both health and social care boundaries. Transformational change is complex, emergent and dynamic requiring new, non-traditional forms of leadership which are highly relational and persuasive.Aim of the studyThe current study aimed to explore a small number of NHS senior leaders’ experiences of undertaking transformational change within their localities over a period of a year following participation in a national transformational change programme designed to enhance personal capabilities.MethodFour pairs of leaders working on different change programmes took part in the study and were interviewed at three time points about their approach to their change work. The data were analysed qualitatively using template analysis.ResultsA core theme ofCreating Allegiance to an Emergent Future Worldwas developed. The senior leaders created allegiance to the transformational change through a process ofConnectingon three levels:relational,with purpose and visionandthrough practice.Allegiance creation was attempted even if the transformational change work at the year-end was deemed successful or not.ConclusionsThe study highlights the types of leadership behaviours employed by the participants reflecting the complexity and social construction of their transformational work. The findings provide further evidence to the existing system leadership literature as well as emphasising the importance of creating stakeholder, multilevel buy-in to healthcare transformation.
Tendon structure quantified using ultrasound imaging differs based on location and training type
Authors: Hagan Kenton L. , Hullfish Todd , Casey Ellen , Baxter Josh R. 
Published: September 2018

Achilles tendinopathy is 10 times more common among running athletes compared with age-matched peers. Load-induced tendon remodeling and its progression in an at-risk population of developing symptomatic tendinopathy are not well understood. The purpose of this study was to prospectively characterize Achilles and patellar tendon structure in competitive collegiate distance runners over different competitive seasons using quantitative ultrasound imaging. Twenty-two collegiate cross-country runners and eleven controls were examined for this study. Ultrasound images of bilateral Achilles and patellar tendons were obtained near the start and end of the collegiate cross-country season and the conclusion outdoor track season. Collagen organization, mean echogenicity, tendon thickness, and neovascularity were determined using well-established image processing techniques. Achilles tendon collagen was less aligned in runners compared with controls (28% greater) but improved slightly (7% decrease) after the completion of the track season. Conversely, patellar tendons in runners were similar to control tendons throughout the cross-country season but underwent collagen alignment (17% decrease) and tendon hypertrophy (21% increase). Our findings indicate that Achilles tendon structure in trained runners differs structurally from control tendons but is stable throughout training while patellar tendon structure changes in response to the transition in training volume between cross-country and track seasons. These findings expand upon prior reports that some degree of tendon remodeling may act as a protective adaptation for sport specific loading. NEW & NOTEWORTHY In this study we prospectively examined the Achilles and patellar tendon structure of distance runners to determine if continued training through multiple seasons elicits tendon remodeling or pathology. We found that Achilles and patellar tendons respond uniquely to the changing loads required during each season. Achilles tendon collagen alignment is mostly stable throughout the competitive cycle, but the patellar tendon structurally remodels following the transition from cross-country to track season.
miR-122-5p Expression and Secretion in Melanoma Cells Is Amplified by the LPAR3 SH3–Binding Domain to Regulate Wnt1
Authors: Byrnes Charnel C. , Jia Wei , Alshamrani Ali A. , Kuppa Sudeepti S. , Murph Mandi M. 
Published: September 2018

AbstractThe lysophosphatidic acid receptor-3 (LPAR3) is a G protein–coupled receptor that mediates viability among malignant cells and aggressiveness among certain tumors. The study's objective was to determine the interplay between LPAR3 and miRNAs to impact key cellular signaling pathways. Using SK-Mel-2 and SK-Mel-5 melanoma cells, wild-type and mutated receptors were stably expressed to explore molecular mechanisms. LPAR3 signaling induced miR-122-5p intracellularly and subsequently its inclusion into exosomes. This amplification resulted in less abundant Wnt1, maintenance of GSK3 inactivation and to a lesser extent, partial degradation of β-catenin. The surge in miR-122-5p and reduction in Wnt1 originated from signaling at the Src homology 3 (SH3) ligand–binding motif within the third intracellular loop of LPAR3, because mutant receptors did not increase miR-122-5p and had a weakened capacity to reduce Wnt1. In addition, a key mediator of melanoma survival signaling, the peroxisome proliferator-activated receptor gamma coactivator 1-α (PPARGC1A/PGC1), was involved in miR-122-5p transcription. In conclusion, this study highlights the powerful role miRNAs have in fine-tuning specific G protein–coupled receptor-mediated signaling events by altering the transcription of signaling transduction pathway components. This study also identifies that LPAR3 increases miR-122-5p expression, which occurs mechanistically through the SH3 domain and helps explain why miR-122-5p increases are detected in cancer patient serum.Implications:LPAR3 is partially responsible for the production and secretion of miR-122-5p, found in the serum of a wide variety of patients with cancer.