bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Hypertension and Its Clinical and Psychological Factors in Type 2 Diabetes Patients in Ghana: A Secondary Analysis of a Cross‐Sectional Study
Authors: Boima V. , Yorke E. , Ganu V. , Twumasi L. , Ekem-Ferguson G. , Dey D. , Kretchy I. A. , Agyabeng K. , Mate-Kole C. C. 
Published: November 2024

Background: Hypertension (HTN) is common in patients with type 2 diabetes mellitus (T2DM). Patients with both T2DM and HTN have a higher risk of heart disease, kidney disorders, and mortality than those with either HTN or T2DM alone. Patients’ psychological well‐being plays a significant role in the optimum management of these chronic conditions. This study is aimed at determining the current prevalence of HTN and its related clinical and psychological factors in patients with T2DM.Methods: This cross‐sectional study was conducted at the Korle‐Bu Teaching Hospital with 156 patients diagnosed with T2DM. A structured questionnaire was used to obtain information on sociodemographic and clinical characteristics. In addition, the following information was obtained from the patients’ clinical files: blood pressure, height, weight, waist circumference, serum creatinine, and urine protein. Depression, resilience, and coping skills of the participants were measured using the Brief Symptom Inventory‐18, Resilience Scale for Adults, and Brief COPE Inventory, respectively. Data were analyzed using STATA version 18, with a significance level of p < 0.05.Results: The median age of respondents was 62.0 (IQR: 51.50, 67.00) years. The majority was female (76.3%). The prevalence of HTN among the patients with T2DM was 79.9% (95% CI: 72.7–85.9). The average body mass index (BMI) of the patients was 28871kg/m2 with 34.8% and 36.2% being overweight and obese, respectively. The average HBA1C level was 8.6 ± 2.1 with 71.8% of the patients having poor glycemic control. Increasing age, caregiver, and personal resilience were factors significantly associated with HTN (p value of <0.05) among patients with T2DM.Conclusion: The prevalence of HTN among T2DM patients was high; age, caregiver, and personal resilience significantly predicted HTN among T2DM patients. These findings have implications for healthcare providers in implementing strategies to reduce central obesity and incorporating resilience as an important factor in improving treatment outcomes in patients with T2DM.
Effects of a Tailored Mobile Messaging Intervention for Indoor Tanning Cessation in Young Females: A Randomized Clinical Trial
Authors: Mays Darren , Alalwan Mahmood A. , Long Lauren , Atkins Michael B. , Tercyak Kenneth P. 
Published: November 2024

Abstract

Background:
Indoor tanning increases the risk of skin cancer and can become addictive. There is little research on indoor tanning cessation interventions.

Methods:
From 2019 to 2022, we conducted a clinical trial (n = 265) testing a tailored mobile messaging cessation intervention in 18- to 30-year-old females screened for indoor tanning addiction. Participants were randomized to a control arm receiving standard risk education or a cessation intervention arm receiving tailored mobile messaging for 4 weeks. The main outcomes were indoor tanning cessation, motivation to quit, quit attempts, and indoor tanning cognitions assessed at the end of treatment and 3 months later.

Results:
At the end of treatment, intervention participants were more likely to report quitting indoor tanning [OR = 2.10, 95% confidence interval (CI) = 0.99–4.44; P &lt; 0.05], but there were no significant differences by 3 months (OR = 1.54, 95% CI = 0.82–2.87; P = 0.17). Intervention participants who did not quit reported higher motivation to quit than control participants at the end of treatment (M = 3.40, SD = 1.72, M = 2.54, SD = 1.63; P &lt; 0.01) and 3 months (M = 3.75, SD = 1.93, M = 2.85, SD = 1.85; P &lt; 0.01).

Conclusions:
Tailored mobile messaging successfully affects indoor tanning cessation behaviors and cognitions in young adult females who meet the screening criteria for tanning addiction.

Impact:
This trial provides preliminary support for the efficacy of a mobile messaging cessation intervention for young adult females who meet the screening criteria for tanning addiction. Results indicate that additional intervention features should be tested to increase the durability of effects.
Abstract A005: Microbind affinity blood filter platform technology with affinity for heparan sulfate rapidly removes circulating epithelial tumor and cancer stem cells while downregulating VEGF A in patients with advanced and refractory solid tumors
Authors: ILIC Sanja , Premuzic Vedran , Ward Robert 
Published: November 2024

Abstract
Background: Vascular endothelial-derived growth factor A(VEGFA) has been proven to be integral in the molecular pathogenesis of metastasis and tumor growth. It is known that VEGF is responsible for encoding a heparin-binding protein, which exists as a disulfide-linked homodimer which serves to promote the propagation and movement of vascular endothelial cells. This is essential for both pathological and physiological angiogenesis. CETC and cancer stem cells are potent secretors of VEGFA which is an essential cytokine that facilitates new vascular vessel formation, thus further enabling metastatic tumor growth. Methods: We developed a new extracorporeal microbind affinity blood filter platform technology with active heparane sulfate receptors (onco seraph). Heparin is cleaved into short chains which are permanently covalently bound to ultrahigh molecular weight polyethylene (UHMWPE) surfaces to create a new composition of matter. Liquid biopsy established high capacity for the rapid removal of CETC and PDAC cancer stem cells. Onco seraph was investigated in a dose escalation phase I/II clinical trial in adult patients with advanced and relapsed/refractory solid (what) and/or PDAC after standard of care and other lines of therapy failed in preventing disease progression. Results: To date 12 patients have been treated with onco seraph. No patient experienced treatment related adverse effects. Best responses include complete responses and stable disease at a low oncoseraph dose. Greatest efficacy was observed for patients whose liquid biopsy revealed significant and rapid reduction of CETC and cancer stem cells, followed by greater than 50% reduction in circulating VEGFA. Conclusions: This data supports the conclusion that the onco seraph platform technology is safe and has a high therapeutic value as an extracorporeal treatment for metastatic solid tumors refractory to standard of care.
Citation Format: Sanja ILIC, Vedran Premuzic, Robert Ward. Microbind affinity blood filter platform technology with affinity for heparan sulfate rapidly removes circulating epithelial tumor and cancer stem cells while downregulating VEGF A in patients with advanced and refractory solid tumors [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A005.
Abstract A023: Advancing RNA liquid biopsy technology via long-read sequencing
Authors: Kim Daniel H. 
Published: November 2024

Abstract
Recent studies have begun to highlight the advantages of RNA-based liquid biopsy technology for cancer early detection, and we sought to further advance the development of RNA liquid biopsies by incorporating single-molecule, long-read sequencing technology. Cell-free RNAs are dynamically secreted by the cells that comprise our tissues and organs but are thought to be largely degraded in the blood. To determine the true length of cell-free RNAs, we performed long-read nanopore sequencing on cell-free RNAs isolated from pancreatic and esophageal cancer patient plasma and found that thousands of genes are detectable as full-length cell-free RNAs in vivo. By creating a custom annotation of the transcriptome for cell-free RNA quantification, we found that cancer patients exhibit high levels of repetitive cell-free RNAs in plasma, with full-length, SINE-derived cell-free RNAs being among the most abundant repetitive RNAs. Moreover, we found that utilizing repetitive cell-free RNA features substantially improved the performance of our machine learning models for cancer classification. Notably, nanopore sequencing enabled us to discover over 250,000 novel cell-free RNAs that have not been previously annotated in the human genome. Our findings highlight the value and utility of long-read nanopore sequencing of cell-free RNAs for discovering and leveraging novel RNA biomarkers for cancer early detection via RNA liquid biopsy technology.
Citation Format: Daniel H. Kim. Advancing RNA liquid biopsy technology via long-read sequencing [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A023.
Abstract A028: Precise monitoring of osteosarcoma patients by noninvasive assessment of matrix metalloproteinases activities in plasma extracellular vesicles
Authors: Lee Junseok , Ji You-Ren , Kim Hyoyong , Chen Zhao , Zhang Ryan , Banuet Diego , Mathkour Yusef , Vo Kenny , Jonas Steven , Zhu Yazhen , Tseng Hsian-Rong 
Published: November 2024

Abstract
Osteosarcoma (OS) is the most prevalent bone cancer in children, characterized by aggressive primary tumors and high metastatic potential. Current treatment protocols involve a combination of neoadjuvant chemotherapy, surgery, and adjuvant chemotherapy, tailored based on histological grade and metastatic risk. Identifying metastatic lesions through routine imaging significantly impacts treatment outcomes, highlighting the need for innovative diagnostic tools for timely monitoring. Noninvasive in vitro diagnostics, analyzing circulating tumor components like tumor-derived extracellular vesicles (EVs), would offer promise for monitoring treatment responses in pediatric sarcomas. Matrix metalloproteinases (MMPs) play a crucial role in tumor progression by degradation of the extracellular matrix; thus, recent research has focused on understanding their functional roles by profiling their proteolytic activities. Tumor EVs are crucial in transporting MMPs to facilitate invasion and metastasis, making them suitable surrogate for noninvasive profiling of MMP activities. Herein, we introduce an innovative OS EV MMP Activity Assay to assess MMP activities in OS patients noninvasively. This assay evaluates six different combinations of surface markers on OS EVs and OS-associated MMPs, generating a unique profile for each patient. Using a logistic regression model, an OS EV MMP Activity Score is generated from the most effective combinations. This Score has shown excellent performance in distinguishing between metastatic and localized OS. In a longitudinal study of six OS patients undergoing treatment, the Score strongly correlated with treatment response as measured by routine imaging. The assay represents a significant advancement in harnessing tumor EVs for diagnostics, providing a noninvasive, quantitative, and reliable approach to assess MMP activities in solid tumors.
Citation Format: Junseok Lee, You-Ren Ji, Hyoyong Kim, Zhao Chen, Ryan Zhang, Diego Banuet, Yusef Mathkour, Kenny Vo, Steven Jonas, Yazhen Zhu, Hsian-Rong Tseng. Precise monitoring of osteosarcoma patients by noninvasive assessment of matrix metalloproteinases activities in plasma extracellular vesicles [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A028.
Abstract A054: Oncogenic functions of alternatively spliced MDM2-ALT2 isoform in retroperitoneal liposarcoma
Authors: de Faria Fernanda Costas C. , Khurshid Safiya , Sarchet Patricia , Tahara Sayumi , Casadei Lucia , Grignol Valerie , Karna Roma , Rentsch Sydney , Sp Nippin , Beane Joal D , Mazzoccoli Luciano , Montes Matias , Nigita Giovanni , Sharick Joe T , Leigth Jennifer L , Calore Federica , Chandler Dawn , Pollock Raphael E 
Published: November 2024

Abstract
Retroperitoneal liposarcoma (RPLPS) is one of the most common histologic subtypes of soft tissue sarcoma (STS). Complete surgical resection remains the mainstay treatment while the high rate of locoregional recurrence constitutes the predominant cause of mortality. Well- differentiated (WDLPS) and dedifferentiated (DDLPS) liposarcoma are the most frequent subtypes of RPLPS and present amplified MDM2 gene as a hallmark. However, there are few reports evaluating the role of alternatively spliced MDM2 transcripts in RPLPS. In this study, we assessed MDM2-ALT2 expression levels in a cohort of RPLPS patients and evaluated biological functions of MDM2-ALT2 isoform in vitro in DDLPS cell lines. Using BaseScope™ and qPCR, we demonstrated that MDM2-Full Length (MDM2-FL) and MDM2-ALT2 expression levels were upregulated in RPLPS patient-derived tissue samples compared to normal adjacent to tumor tissue (NAT). DDLPS cells overexpressing MDM2-FL or MDM2-ALT2 had higher proliferation rates and increased migration and invasion capacities, as well as increased protein levels of p- AKT, mTOR, p70S6K, MMP2 and cJun. Simultaneous overexpression of MDM2-ALT2 and AKT silencing showed that AKT inhibition impaired p-p70S6K and MMP2 protein increased levels and led to significantly decreased proliferation and migration rates compared to cells overexpressing MDM2-ALT2 only. Taken together, our data suggest that MDM2-ALT2 may promote RPLPS progression.
Citation Format: Fernanda Costas C. de Faria, Safiya Khurshid, Patricia Sarchet, Sayumi Tahara, Lucia Casadei, Valerie Grignol, Roma Karna, Sydney Rentsch, Nippin Sp, Joal D Beane, Luciano Mazzoccoli, Matias Montes, Giovanni Nigita, Joe T Sharick, Jennifer L Leigth, Federica Calore, Dawn Chandler, Raphael E Pollock. Oncogenic functions of alternatively spliced MDM2-ALT2 isoform in retroperitoneal liposarcoma [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A054.
Abstract A057: Cancer-induced epigenomic changes in circulating immune cells enable breast, colorectal and lung cancer detection
Authors: Guler Gulfem D , Mognol Giuliana P , Ning Yuhong , Fraire Carolina , Haan David , Kesling Michael , Malta Roger , Peters Melissa , Bergamaschi Anna , Phillips Tierney , Chowdhury Shimul , Volkmuth Wayne , Levy Samuel 
Published: November 2024

Abstract
Background: Epigenomic changes via the measurement of 5-hydroxymethylation cytosine (5hmC) in DNA have been shown to enable detection in a variety of cancers including pancreatic and ovarian cancer in plasma-derived cell-free DNA (cfDNA). Here, we investigated whether blood-based 5hmC profiling of genomic DNA (gDNA) derived from cells of the buffy coat (BC) possess signals that enable the detection of breast, colorectal and lung cancers. Method: Blood samples collected in Streck tubes from cancer patients or non-cancer controls were processed to obtain buffy coat and plasma samples. gDNA and cfDNA was isolated from buffy coat and plasma, respectively. gDNA or cfDNA was then subjected to 5hmC enrichment by click chemistry followed by preparation of sequencing libraries. Sequencing was performed to obtain genome-wide 5hmC profiles of buffy coat gDNA and cfDNA samples. Results: Differential hydroxymethylation analysis of BC gDNA revealed increased 5hmC over genes associated with myeloid lineage in breast, colorectal and lung cancers relative to non-cancer controls. Flow cytometry analysis confirmed increases in myeloid cells in cancer samples relative to non-cancer controls. 5hmC patterns in BC gDNA showed a larger number of cancer-associated changes compared to cfDNA, in particular this was found in early-stage disease. 5hmC features from BC gDNA or plasma-derived cfDNA were used to build prediction models that yielded cancer classification with overall sensitivity of 51.3% and 52.6% at 98% specificity, for BC or cfDNA, respectively. For early-stage cancer, the sensitivity at 98% specificity was 46.5% for the BC and 28.2% for cfDNA model. A final prediction model incorporating features from both tissue sources achieved superior classification (65.8% sensitivity) at 98% specificity, compared with using features from either tissue source alone, particularly for early-stage cancer which achieved 53.5% sensitivity of cancer detection. Conclusion: Our results demonstrate that cancer impacts the 5hmC profiles of buffy coat cells which can be leveraged for cancer detection using blood.
Citation Format: Gulfem D Guler, Giuliana P Mognol, Yuhong Ning, Carolina Fraire, David Haan, Michael Kesling, Roger Malta, Melissa Peters, Anna Bergamaschi, Tierney Phillips, Shimul Chowdhury, Wayne Volkmuth, Samuel Levy. Cancer-induced epigenomic changes in circulating immune cells enable breast, colorectal and lung cancer detection [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A057.
Abstract A067: lncTransformer: Identifying novel prognostic biomarkers for pancreatic cancer using deep learning models
Authors: Singh Shivali 
Published: November 2024

Abstract
Pancreatic cancer remains a formidable challenge due to high mortality rates and limited therapeutic options due to late detection of the cancer. Recent literature has focused on investigating the potential of long noncoding RNAs (lncRNAs) as biomarkers in several cancers. To identify significantly altered expression of lncRNA in pancreatic cancer, I collected information from the Cancer Genome Atlas (TCGA) and extracted RNA-sequencing (RNA-seq) transcriptomic profiles of pancreatic carcinomas. Out of 60,660 gene transcripts shared between 151 pancreatic cancer patients, 38 lncRNAs were identified to be significantly differentially expressed. To find the lncRNAs related to metastatic progression of pancreatic cancer, different machine learning algorithms, including logistic regression (LR), support vector machine (SVM), random forest classifier (RFC), and a custom autoencoder model were trained to identify potential prognostic biomarkers. The custom deep learning model predicted metastatic prognostic biomarkers with a 92% accuracy, with the second-best accuracies coming from the SVM and RFC models, having an accuracy of 76%. Using the deep learning model, the following novel lncRNA and gene biomarkers were identified: LINC01300, SERPINB13, AC010789.1, TMPRSS15, DUSP5-DT, AL513128.3, MIR205HG, LINC00486, RF00019, LINC01115, and AC133530.1. Further investigating these results, specifically the identified lncRNA, the LINC01300 was identified to be potentially circulating in the blood. If further analysis proves this hypothesis correct then LINC01300 along with other identified genes could serve as a biomarker panel for a liquid biopsy. The identified biomarkers would be especially helpful in rural areas with lower accessibility to healthcare as it would allow for an easier, more efficient, and cost-effective method of pancreatic cancer detection. Based on these findings, further investigations of this gene panel are being conducted, in vitro and in vivo, to create a novel liquid biopsy to assist in the prognostic diagnosis of metastatic progression of pancreatic cancer.
Citation Format: Shivali Singh. lncTransformer: Identifying novel prognostic biomarkers for pancreatic cancer using deep learning models [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A067.
Abstract B041: High purity CTC RNA sequencing identifies poor prognosis lineage states in castrate resistant prostate cancer
Authors: Sharifi Marina N , Sperger Jamie M , Taylor Amy K , Tippins Katharine E , Reese Shannon R , Carreno Viridiana , Kaufmann Katherine R , Chang Alex H , Nunamaker Luke A , Linebarger Charlotte , Helzer Kyle T , Bootsma Matthew , Blitzer Grace C , Floberg John , Kosoff David , McKay Rana R , Wei Xiao X , Zhao Shuang G , Lang Joshua M 
Published: November 2024

Abstract
This abstract is being presented as a short talk in the scientific program. A full abstract is printed in the Proffered Abstracts section (PR010) of the Conference Program/Proceedings.
Citation Format: Marina N Sharifi, Jamie M Sperger, Amy K Taylor, Katharine E Tippins, Shannon R Reese, Viridiana Carreno, Katherine R Kaufmann, Alex H Chang, Luke A Nunamaker, Charlotte Linebarger, Kyle T Helzer, Matthew Bootsma, Grace C Blitzer, John Floberg, David Kosoff, Rana R McKay, Xiao X Wei, Shuang G Zhao, Joshua M Lang. High purity CTC RNA sequencing identifies poor prognosis lineage states in castrate resistant prostate cancer [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr B041.
Abstract B070: Dissecting EV dynamics in the sequestration of doxorubicin from breast cancer cells
Authors: Halvaei Sina , Xu Jing , Chittaranjan Suganthi , Gorski Sharon , Williams Karla 
Published: November 2024

Abstract
Background: Extracellular vesicles (EVs) are one of the three main analytes sought for in liquid biopsy development. EVs are released by all cell types including cancer cells and play a crucial role in cell-cell communication. Breast cancer cells have been shown to utilize EVs as a mechanism to sequester chemotherapeutic agents, thereby conferring resistance to treatment. This study investigates the role of EVs in doxorubicin (DOX) resistance in MDA-MB-231 breast cancer cells. Method: MDA-MB-231 cells were exposed to increasing dose of anthracycline for 6 and 12 months. Resistance was confirmed by cytotoxicity assay and western blot. Resistant and parent cells were labeled for immunohistochemistry and their EVs were isolated via ultracentrifugation. EVs were analysed using nanoscale flow cytometry and sent for mass spectrophotometry. Result: Using nanoscale flow cytometry, we optimized a method to detect DOX-carrying EVs based on the intrinsic fluorescence of the drug. We found that DOX-resistant cells exhibited lower intracellular DOX accumulation and higher expression of the EV marker CD63 compared to their parental counterparts. Conclusion: These results suggest that chemoresistant breast cancer cells may alter their EV production machinery to evade the lethal effects of DOX. Understanding this mechanism could lead to novel strategies to overcome chemoresistance in breast cancer.
Citation Format: Sina Halvaei, Jing Xu, Suganthi Chittaranjan, Sharon Gorski, Karla Williams. Dissecting EV dynamics in the sequestration of doxorubicin from breast cancer cells [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr B070.