bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of Dehydrated Human Amnion/Chorion Membrane Allografts in More Than 100 Patients with Six Major Types of Refractory Nonhealing Wounds
Authors: Garoufalis Matthew , Nagesh Darshan , Sanchez Patrick J. , Lenz Robin , Park Sarah J. , Ruff Jake G. , Tien Anna , Goldsmith Justin , Seat Andrea 
Published: April 2018

Background:
Biochemical properties of the amniotic membrane help modulate inflammation and enhance soft-tissue healing. In controlled trials, the efficacy of dehydrated human amnion/chorion membrane (dHACM) allografts has been established. Our purpose is to describe our experience with using dHACM to treat nonhealing wounds of various etiologies.

Methods:
We conducted a retrospective review of deidentified data from 117 consecutive patients treated in an outpatient clinic with dHACM allografts with wounds of various etiologies over 2 years. The decision to use advanced wound-care treatments is based on rate of healing observed after initiation of standard wound care and patient risk factors. Eligibility for treatments such as amniotic membrane allografts includes wounds without 50% reduction after 4 weeks, or earlier in patients deemed to be at high risk for nonhealing or with a history of chronic wounds. In micronized or sheet formulation, dHACM is applied to the wound weekly after sharp/mechanical debridement as necessary, and wound-care practices appropriate for wound type and location are continued.

Results:
Thirty-four percent of allograft recipients had diabetic foot ulcers, 25% had venous leg ulcers, 20% had surgical wounds, 14% had pressure ulcers, 6% had ischemic wounds, and 2% had traumatic wounds. Complete healing occurred in 91.1% of treated patients, with a mean ± SD number of weekly applications per healed wound of 5.1 ± 4.2.

Conclusions:
In addition to wounds of diabetic origin, dHACM can significantly expedite healing in refractory wounds of varying etiologies.
EXPLICITAÇÃO DOS DISCURSOS DE CRIMES COM SUSPEITA DE MOTIVAÇÃO HOMOFÓBICA EM JORNAIS DO ESTADO DO AMAZONAS
Authors: Toscano Isaac Guidão , Melo Lucilene Ferreira 
Published: April 2018

<p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: justify;"><span style="font-size: 12pt; font-family: 'Times New Roman', serif;">Objetiva-se analisar os discursos sobre homicídios a pessoas não heterossexuais, noticiados nos Estado do amazonas no período de 2010 á 2012.</span><span style="font-size: 12.0pt; font-family: "> A pesquisa ancorou-se no método de análise do discurso a partir da arqueogenealogia foucaultiana em diálogo com a produção de pesquisadores brasileiros e estrangeiros filiados à Teoria<em> Queer. </em> </span><span style="font-size: 12pt; font-family: 'Times New Roman', serif;">Evidenciou-se </span><span style="font-size: 12.0pt; font-family: ">a propalação do discurso jornalístico presente nos crimes suspeitos de motivação homofóbica através dos jornais: A crítica e Diário 24 horas, em versões impressas e <em>on line</em>. Dentre os resultados destaca-se a sutil reiteração de enunciados estigmatizantes das sexualidades não heterossexuais, tais como a repatologização e a moralização da conduta sexual presentes no discurso dos jornais amazonenses. Evidenciam-se ainda, as estratégias jornalísticas de reiteração de verdades dos seres abjetos à heteronormatividade.<strong></strong></span></p>
Cotas Raciais na UFSC: Trajetórias e Projetos em um novo Campo de Possibilidades
Authors: Sagaz Gabriela Solange 
Published: April 2018

<p>As ações afirmativas enquanto Cotas Raciais foram inseridas no vestibular da Universidade Federal de Santa Catarina no ano de 2007. Em 2012, a Lei 12.711/12 aprovada pela Câmara Federal realizou modificações significativas nas Ações Afirmativas da Universidade. Este artigo objetiva, através da análise de <em>entrevistas narrativas</em>, apresentar as trajetórias escolares, <em>projetos</em> e consequências na vida dos alunos cotistas que optaram por cotas raciais no vestibular da UFSC. Sugere-se que as Cotas Raciais não apenas viabilizam a concretização de <em>projetos </em>individuais, mas ampliam o <em>campo de possibilidades</em> e os <em>projetos </em>destes jovens. </p>
A Posição da Escrita Feminina no Cânone Literário Brasileiro: analisando Uma História da Poesia Brasileira, de Alexei Bueno
Authors: Saraiva Eduardo De Souza 
Published: April 2018

<p><strong> </strong>O presente trabalho propõe discutir a posição da escrita de autoria feminina no Brasil. Para tanto, apresentaremos no primeiro momento um breve contexto da gênese da escrita feminina, logo em seguida, abordaremos algumas questões sobre o cânone e por fim analisaremos o livro <em>Uma história da poesia brasileira,</em> de Alexei Bueno (2007) no qual verificaremos o espaço dado à produção literária poética feminina no Brasil, e consequentemente, a inclusão ou não de textos produzidos por mulheres. </p>
A interface entre Gênero e Direito: entrevista com Alda Facio
Authors: Da Silva Salete Maria , Wright Sonia Jay , Silva Júnior Enézio De Deus 
Published: April 2018

<p><span>No dia 13 de setembro de 2016, em Salvador, Bahia, mais precisamente na residência da professora Sonia Jay, onde a jurista Alda Facio esteve hospedada, três integrantes do grupo de pesquisa Jusfemina realizaram a presente entrevista, cujo conteúdo versa, dentre outros assuntos, sobre a interface entre gênero e direito e a trajetória da jurista feminista Alda Facio. Ao longo do diálogo, que durou cerca de quarenta minutos, buscou-se registrar sua contribuição para o feminismo jurídico latino americano e o papel das feministas na transformação do mundo jurídico, conforme passamos a expor.</span></p>
Paleomagnetic Secular Variation Constraints on the Rapid Eruption of the Emeishan Continental Flood Basalts in Southwestern China and Northern Vietnam
Authors: Xu Yingchao , Yang Zhenyu , Tong Ya‐Bo , Jing Xianqing 
Published: April 2018

AbstractEstimating the duration of magma eruptions using isotopic dating methods is difficult because of the intrinsic errors of the technique regarding the dated materials (such as zircon). However, the long‐term variation of the geomagnetic field recorded by lava flows can be used to estimate the net duration of an eruption sequence. The Emeishan basalts at Dongchuan, with a thickness of 630 m, yielded a reliable characteristic remanent magnetization of normal polarity and which passed the fold test (Tauxe & Watson, 1994,https://doi.org/10.1016/0012-821X(94)90006-X). Stratigraphic and magnetostratigraphic correlations of the Emeishan basalts in the Dongchuan section with other sections indicate that the eruption of the Emeishan basalts at Dongchuan spans the entire normal polarity zone in the early stage of the Emeishan large igneous province. A flow‐by‐flow analysis of geomagnetic directions of the Emeishan basalts at Dongchuan indicates that four directional groups and fifteen individual lava directions were recorded, with a net duration (excluding quiescent intervals) of no more than 3100 years. The averaged site directions from the Emeishan basalts with normal polarity conforming to a geocentric axial dipole direction indicate that this interval is longer than 104–105 years. In addition, a magnetostratigraphic study indicates that the normal polarity interval recorded by the Emeishan basalts was shorter than 2–20 × 104 years. Thus, the total duration of the normal polarity stage of the Emeishan large igneous province was roughly 105 years. There is a possible relationship between the rapid eruption and the Late Capitanian mass extinction (259.8 ± 0.4 Ma, Henderson et al., 2012).
Quantifying Climate‐Related Interactions in Shallow and Deep Storage and Evapotranspiration in a Forested, Seasonally Water‐Limited Watershed in the Southeastern United States
Authors: Aulenbach Brent T. , Peters Norman E. 
Published: April 2018

AbstractThe Southeastern United States experiences recurring hydrological droughts, which can reduce water availability and result in water deficits. Long‐term monitoring data from Panola Mountain Research Watershed, a small, forested, seasonally water‐limited watershed near Atlanta, GA, was used to quantify interactions of climatic variability with shallow and deep storage and evapotranspiration. Watershed storage (WS) and actual evapotranspiration (AET) were estimated monthly from 1985 through 2015 using a relationship with baseflow and a water‐budget approach, respectively. A watershed shallow storage (SS) water budget, based on soil moisture profile data, indicated a transition from recharge to surplus as dormant season SS increased to field capacity, and a transition from utilization of stored water to water deficits as growing season SS declined toward its wilting point. Deeper storage appeared unavailable to AET during dry conditions. The majority of deeper storage recharge occurred during the dormant season and required SS to be near field capacity. WS was an effective drought indicator. Growing season droughts typically occurred when WS was below normal at the end of the dormant season and growing season precipitation (P) was below or near normal. A hydrologic persistence analysis showed that monthly month‐standardized WS was significantly correlated (p‐value < 0.05) with past monthly month‐standardized WS for the previous 19 months and with past monthly P for the previous 11 months, indicating the importance of past hydrologic conditions on WS. These findings allowed assessment of expected climatic changes on the water cycle, which included effects on future recharge, water deficits, and drought occurrence.
Retreat to refugia: Severe habitat contraction projected for endemic alpine plants of the Olympic Peninsula
Authors: Wershow Samuel T. , DeChaine Eric G. 
Published: April 2018

PREMISE OF THE STUDYThe unique geography of the Olympic Peninsula in Washington State, USA has promoted a flora with exceptional endemism that may face high rates of extinction due to climate change. The Olympic Mountains provide an unparalleled opportunity to test predicted responses in topographically complex environments and inform conservation plans for threatened alpine taxa.METHODSWe estimated the potential impact of climate change for five endemic alpine plants of the Olympic Peninsula. We used Principal Components Analysis (PCA) to visualize changes in climate niche‐space in the alpine zone and the exposure of the endemics to changing conditions. We then constructed climate and topographic‐based Species Distribution Models (SDMs) to estimate changes in the distribution of habitat for each taxon.KEY RESULTSThe Olympic alpine zone will experience novel environmental conditions in the future, with a pronounced decrease in winter snow and an increase in growing‐season moisture stress. When topography was taken into account, the losses were more dramatic than the impact of climate alone. Indeed, 85–99% of the suitable habitat will be lost for each of the five focal taxa by 2080, with thermal refugia remaining only on the highest peaks of the eastern Olympics.CONCLUSIONSThe Olympic alpine endemics are stranded on ever‐shrinking habitat islands. Within a few, isolated thermal refugia, the distribution of micro‐topography may be a critical factor in determining long‐term survival. The Olympic Mountains and their alpine taxa are a model system for endemics worldwide, illustrating the biogeographic characteristics that underpin vulnerability to climate change.
Evidence‐based dosing of maintenance subcutaneous immunotherapy: a contemporary review of state‐of‐the‐art practice
Authors: Hoover Hunter , Leatherman Bryan , Ryan Matthew , McMains Kevin , Veling Maria 
Published: April 2018

BackgroundSubcutaneous immunotherapy is an effective allergy treatment only if properly dosed. In this article we review the data on the probable effective dose range for subcutaneous immunotherapy and convert the recommended doses into a clinically relevant format.MethodsA comprehensive literature search of dose‐response subcutaneous immunotherapy studies was done of EBM databases, Medline database, PreMedline, and the National Guideline Clearinghouse for the period 1980–2016. Recommended doses were converted to the volume of allergen extract that should be added to a 5‐mL maintenance vial.ResultsA safe and effective dose for subcutaneous immunotherapy is likely 5–20 μg of major allergen per injection. A 0.5‐mL injection from a 5‐mL maintenance vial containing 0.2 mL of manufacturer's extract of each allergen should reach the lower end of the probable effective dose range for most allergens. A larger volume of extract is required to reach that range when treatment includes cat, dog, or only 1 dust mite. Increasing beyond the commonly prescribed 0.2 mL of manufacturer's extract added to a 5‐mL treatment vial is reasonable for nearly all allergens to achieve a maintenance dose higher in the probable effective dose range.ConclusionCurrent otolaryngic allergy practice usually escalates patients to 0.5‐mL injections from 5‐mL maintenance vials containing 0.2 mL of manufacturer's extract of each allergen. With the main exceptions of cat and dog, those injections administered 1 or 2 times per month likely provide an efficacious dose of allergen and are consistent with published guidelines. A larger volume of extract should be considered in certain clinical situations.
O2‐Mediated Oxidation of Aminoboranes through 1,2‐N Migration
Authors: Rauser Marian , Warzecha Daniel P. , Niggemann Meike 
Published: April 2018

AbstractIn analogy to the classical reaction of C−B bonds with peroxides, the first oxidative functionalization of aminoboranes through a 1,2‐N migration was realized. Readily available aliphatic nitro compounds are thereby transformed into N‐ and O‐functionalized hydroxylamines in a single synthetic operation. Addition of hazardous peroxides is avoided. Instead, the insertion of O2, as the terminal oxidant, into Zn−C bonds provides the necessary peroxides. The required zinc organyls, in turn, are formed through a boron‐to‐zinc exchange, from an organoboronic ester byproduct of the nitro‐to‐aminoborane transformation.
A Facile Method for Producing Selenocysteine‐Containing Proteins
Authors: Mukai Takahito , Sevostyanova Anastasia , Suzuki Tateki , Fu Xian , Söll Dieter 
Published: April 2018

AbstractSelenocysteine (Sec, U) confers new chemical properties on proteins. Improved tools are thus required that enable Sec insertion into any desired position of a protein. We report a facile method for synthesizing selenoproteins with multiple Sec residues by expanding the genetic code of Escherichia coli. We recently discovered allo‐tRNAs, tRNA species with unusual structure, that are as efficient serine acceptors as E. coli tRNASer. Ser‐allo‐tRNA was converted into Sec‐allo‐tRNA by Aeromonas salmonicida selenocysteine synthase (SelA). Sec‐allo‐tRNA variants were able to read through five UAG codons in the fdhF mRNA coding for E. coli formate dehydrogenase H, and produced active FDHH with five Sec residues in E. coli. Engineering of the E. coli selenium metabolism along with mutational changes in allo‐tRNA and SelA improved the yield and purity of recombinant human glutathione peroxidase 1 (to over 80 %). Thus, our allo‐tRNAUTu system offers a new selenoprotein engineering platform.
Searching ECOD for Homologous Domains by Sequence and Structure
Authors: Schaeffer R. Dustin , Liao Yuxing , Grishin Nick V. 
Published: April 2018

AbstractECOD is a database of evolutionary domains from structures deposited in the PDB. Domains in ECOD are classified by a mixed manual/automatic method wherein the bulk of newly deposited structures are classified automatically by protein‐protein BLAST. Those structures that cannot be classified automatically are referred to manual curators who use a combination of alignment results, functional analysis, and close reading of the literature to generate novel assignments. ECOD differs from other structural domain resources in that it is continually updated, classifying thousands of proteins per week. ECOD recognizes homology as its key organizing concept, rather than structural or sequence similarity alone. Such a classification scheme provides functional information about proteins of interest by placing them in the correct evolutionary context among all proteins of known structure. This unit demonstrates how to access ECOD via the Web and how to search the database by sequence or structure. It also details the distributable data files available for large‐scale bioinformatics users. © 2018 by John Wiley & Sons, Inc.
Live‐Cell Imaging and Analysis with Multiple Genetically Encoded Reporters
Authors: Pargett Michael , Albeck John G. 
Published: April 2018

AbstractGenetically encoded live‐cell reporters measure signaling pathway activity at the cellular level with high temporal resolution, often revealing a high degree of cell‐to‐cell heterogeneity. By using multiple spectrally distinct reporters within the same cell, signal transmission from one node to another within a signaling pathway can be analyzed to quantify factors such as signaling efficiency and delay. With other reporter configurations, correlation between different signaling pathways can be quantified. Such analyses can be used to establish the mechanisms and consequences of cell‐to‐cell heterogeneity and can inform new models of the functional properties of signaling pathways. In this unit, we describe an approach for designing and executing live‐cell multiplexed reporter experiments. We also describe approaches for analyzing the resulting time‐course data to quantify correlations and trends between the measured parameters at the single‐cell level. © 2018 by John Wiley & Sons, Inc.
Elimination of Mitochondrial DNA from Mammalian Cells
Authors: Khozhukhar Natalya , Spadafora Domenico , Rodriguez Yelitza , Alexeyev Mikhail 
Published: April 2018

AbstractTo cope with DNA damage, mitochondria developed a pathway by which severely damaged or unrepairable mitochondrial DNA (mtDNA) molecules are abandoned and degraded, and new molecules are resynthesized using intact templates, if available. In this unit, we describe a method that harnesses this pathway to completely eliminate mtDNA from mammalian cells by transiently overexpressing the Y147A mutant of human uracil‐N‐glycosylase (mUNG1). We also provide an alternate protocol for mtDNA depletion using combined treatment with ethidium bromide (EtBr) and dideoxycytidine (ddC). Support protocols detail approaches for (1) genotyping ρ° cells of human, mouse, and rat origin by PCR; (2) quantitation of mtDNA by quantitative PCR (qPCR); and (3) preparation of calibrator plasmids for mtDNA quantitation. © 2018 by John Wiley & Sons, Inc.
PCR Amplification of Base‐Modified DNA
Authors: Eremeeva Elena , Herdewijn Piet 
Published: April 2018

AbstractAn efficient PCR amplification of various templates (short 57‐mer, random 67‐ and 82‐mer, and long DNA) with base‐modified nucleoside triphosphates is presented here. Using 5‐substituted pyrimidine and 7‐substituted‐7‐deaza‐ or 8‐substituted purine nucleoside triphosphates as substrates for thermostable DNA polymerases [Taq and Vent (exo–)], successful PCR amplification of partially or entirely modified DNA libraries and long DNA constructs (up to 1.5 kb) is achieved. Visualization of double‐stranded PCR product formation is improved through the use of primers with different fluorescent labels. This allows one to monitor the efficiency of modified substrate incorporation and the enzymatic recognition of the modified template during PCR. The redesigned fully base‐modified DNA (denoted ‘DZA’) can be utilized for the straightforward production of diverse libraries for in vitro selection of aptamer and catalytic nucleic acids as well as for the synthesis of artificial genetic templates, replicons, or complex vectors. © 2018 by John Wiley & Sons, Inc.
Categorization of weathering stresses for photovoltaic modules
Authors: Koehl Michael , Heck Markus , Wiesmeier Stefan 
Published: April 2018

AbstractSolar energy conversion requires permanent outdoor operation of essential components such as photovoltaic modules, solar collectors, or reflectors. They are exposed to weathering stresses. The stress levels depend on the local climates. Monitoring of climatic properties and sample properties in different climatic zones (alpine, arid, maritime, moderate, and tropical) during several years provided the base for categorization of these climates. The local climate does not differ very much from year to year in terms of frequency distributions, but big differences are found for different regions or climatic zones. Especially, the solar irradiation is very location‐dependent. The alpine location shows the highest UV irradiation. The UV fraction of the solar irradiation is varying between 2.2% (tropics) and 4.7% (alps). The temperature histograms can be modeled by Gaussian distribution functions. The maritime histogram is very slim and high, showing the cooling by the sea and the wind. Several approaches for a categorization of these local climates can be applied: Mean temperatures, effective temperatures, or the corresponding constant testing time for fictive degradation processes with arbitrary activation energy. The categorization of the relative humidity revealed humid climates in the alpine and the tropical test site, when considering the time of wetness (rh > 80%). Obviously, the humidity stress or the corrosivity would be very different at those sites. Therefore, a more holistic approach considering more stress factors simultaneously would be more appropriate for the categorization. The interaction between the samples and the climate creates the so‐called micro‐climate which is the real stress on the samples, such as surface temperature, daily temperature cycles, surface humidity, or temperature‐enhanced photo‐degradation. The conditions for accelerated life testing (ALT) modeled on the base of monitored climatic data and sample temperatures are different for the different locations. They offer another possibility for categorization of the climatic stresses and the option for designing climate‐adapted components.