bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cultivable microbial community in 2-km-deep, 20-million-year-old subseafloor coalbeds through ~1000 days anaerobic bioreactor cultivation
Authors: Imachi Hiroyuki , Tasumi Eiji , Takaki Yoshihiro , Hoshino Tatsuhiko , Schubotz Florence , Gan Shuchai , Tu Tzu-Hsuan , Saito Yumi , Yamanaka Yuko , Ijiri Akira , Matsui Yohei , Miyazaki Masayuki , Morono Yuki , Takai Ken , Hinrichs Kai-Uwe , Inagaki Fumio 
Published: February 2019

AbstractRecent explorations of scientific ocean drilling have revealed the presence of microbial communities persisting in sediments down to ~2.5 km below the ocean floor. However, our knowledge of these microbial populations in the deep subseafloor sedimentary biosphere remains limited. Here, we present a cultivation experiment of 2-km-deep subseafloor microbial communities in 20-million-year-old lignite coalbeds using a continuous-flow bioreactor operating at 40 °C for 1029 days with lignite particles as the major energy source. Chemical monitoring of effluent samples via fluorescence emission-excitation matrices spectroscopy and stable isotope analyses traced the transformation of coalbed-derived organic matter in the dissolved phase. Hereby, the production of acetate and 13C-depleted methane together with the increase and transformation of high molecular weight humics point to an active lignite-degrading methanogenic community present within the bioreactor. Electron microscopy revealed abundant microbial cells growing on the surface of lignite particles. Small subunit rRNA gene sequence analysis revealed that diverse microorganisms grew in the bioreactor (e.g., phyla Proteobacteria, Firmicutes, Chloroflexi, Actinobacteria, Bacteroidetes, Spirochaetes, Tenericutes, Ignavibacteriae, and SBR1093). These results indicate that activation and adaptive growth of 2-km-deep microbes was successfully accomplished using a continuous-flow bioreactor, which lays the groundwork to explore networks of microbial communities of the deep biosphere and their physiologies.
Impact of gynecologic cancer on pelvic floor disorder symptoms and quality of life: an observational study
Authors: Neron Mathias , Bastide Sophie , Tayrac Renaud de , Masia Florent , Ferrer Catherine , Labaki Majd , Boileau Laurent , Letouzey Vincent , Huberlant Stephanie 
Published: February 2019

AbstractThe objective of our observational prospective study was to investigate the severity and prevalence of urinary and pelvic floor disorders in gynecologic cancer survivors. All patients surviving gynecological cancer in the region as well as women receiving invitations to attend breast-screening checkups as the control population were asked to fill-in questionnaires assessing pelvic prolapse symptoms (PFDI-20, Wexner) and associated quality of life (PFIQ-7). Eighty-nine women were included in the cancer survivor group and 1088 in the control group. Pelvic floor symptoms (PFDI-20 questionnaire) were significantly worse in cancer survivors than in control women (score: 33.3 [14.6–74.1] vs. 20 [4.2–50.0], p = 0.0003). Urge incontinence was significantly worse in cancer survivors in both univariable (ORb = 2.061 [95% CI = 1.284–3.309], p = 0.0027) and multivariable analyses (ORa = 1.672 [95% CI = 1.014–2.758], p = 0.0442), as was fecal incontinence in univariable (ORb = 3.836 [95% CI = 1.710–8.602], p = 0.0011) and in multivariable (ORa = 3.862 [95% CI = 1.657–9.001], p = 0.0018) analyses. Women with benign hysterectomies had poorer quality of life and increased pelvic floor disorders compared to women with no history of surgery. Survivors of gynecological cancer experience significantly more pelvic floor symptoms and an associated reduction in quality of life.
Transparent Display using a quasi-array of Si-SiO2 Core-Shell Nanoparticles
Authors: Dolatyari Mahboubeh , Jafari Ali , Rostami Ali , Klein Axel 
Published: February 2019

AbstractA novel type of transparent monitor with high-resolution images based on Si-SiO2 core-shell nanoparticles is presented in this contribution. In this monitor, a quasi-array of nanoparticles was used to obtain a very sharp scattering profile. For this purpose, the Si-SiO2 nanoparticles were synthesized and with controlling the size of particles, the dominant emission wavelength was controlled. For the fabrication of a blue color transparent monitor the solution processed Si-SiO2 nanoparticles were dispersed in polystyrene and then coated on a transparent glass surface. After drying the film, the typical features representing a transparent monitor were studied. A video projector was used and text and pictures were sent on the monitor. This monitor reveals very attractive features such as simplicity, wide viewing angle, scalability to larger sizes and low cost. Importantly, the texts and pictures can be well presented on both sides of the fabricated monitor. The composite thin film can be also separated from the glass and can be used as a flexible display. To shed light on the impact of the structure on the optical properties Si-SiO2 and Ag nanomaterials representing perfect arrays of nanoparticles, quasi-arrays and randomly oriented nanoparticles were calculated/simulated using the finite-difference time-domain (FDTD) method. The results were compared to the experimental data and show a high accordance.
Maximizing Network Resilience against Malicious Attacks
Authors: Li Wenguo , Li Yong , Tan Yi , Cao Yijia , Chen Chun , Cai Ye , Lee Kwang Y. , Pecht Michael 
Published: February 2019

AbstractThe threat of a malicious attack is one of the major security problems in complex networks. Resilience is the system-level self-adjusting ability of a complex network to retain its basic functionality and recover rapidly from major disruptions. Despite numerous heuristic enhancement methods, there is a research gap in maximizing network resilience: current heuristic methods are designed to immunize vital nodes or modify a network to a specific onion-like structure and cannot maximize resilience theoretically via network structure. Here we map complex networks onto a physical elastic system to introduce indices of network resilience, and propose a unified theoretical framework and general approach, which can address the optimal problem of network resilience by slightly modifying network structures (i.e., by adding a set of structural edges). We demonstrate the high efficiency of this approach on three realistic networks as well as two artificial random networks. Case studies show that the proposed approach can maximize the resilience of complex networks while maintaining their topological functionality. This approach helps to unveil hitherto hidden functions of some inconspicuous components, which in turn, can be used to guide the design of resilient systems, offer an effective and efficient approach for mitigating malicious attacks, and furnish self-healing to reconstruct failed infrastructure systems.
Codonopsis lanceolata attenuates allergic lung inflammation by inhibiting Th2 cell activation and augmenting mitochondrial ROS dismutase (SOD2) expression
Authors: Seo Yun-Soo , Kim Hyo Seon , Lee A. Yeong , Chun Jin Mi , Kim Sung Bae , Moon Byeong Cheol , Kwon Bo-In 
Published: February 2019

AbstractAllergic asthma is a chronic inflammatory disease induced by the inhalation of allergens, which trigger the activation of T helper type 2 (Th2) cells that release Th2 cytokines. Recently, herbal medicines are being considered a major source of novel agents to treat various diseases. In the present study, we evaluated the anti-asthmatic effects of aCodonopsis lanceolataextract (CLE) and the mechanisms involved in its anti-inflammatory effects. Treatment with CLE reduced infiltration of inflammatory cells, especially eosinophils, and the production of mucus in lung tissues. Levels of Th2 cytokines, such as IL-4, IL-5, and IL-13, and chemokines were also decreased following treatment with CLE. Moreover, Th2 cell proportionin vivoand differentiationin vitrowere reduced as evidenced by the decreased expression of GATA3+. Furthermore, the expression of superoxide dismutase (SOD)2, a mitochondrial ROS (mROS) scavenger, was increased, which was related to Th2 cell regulation. Interestingly, treatment with CLE increased the number of macrophages in the lungs and enhanced the immune-suppressive property of macrophages. Our findings indicate that CLE has potential as a novel therapeutic agent to inhibit Th2 cell differentiation by regulating mROS scavenging.
Myeloid-derived suppressor cells in the patients with liver resection for hepatitis B virus-related hepatocellular carcinoma
Authors: Lee Wei-Chen , Wang Yu-Chao , Cheng Chih-Hsien , Wu Tsung-Han , Lee Chen-Fang , Wu Ting-Jung , Chou Hong-Shiue , Chan Kun-Ming 
Published: February 2019

AbstractLiver resection remains the popular treatment for hepatocellular carcinoma (HCC). The aim of this study was to explore the alteration of immune cells in HCC patients with liver resections. Nineteen patients were included and their peripheral blood samples were taken before and after liver resections for immune-cell analysis. The clinical characteristics showed that the median diameter of the resected tumors was 7.5 cm with a range from 1.4 to 16.5 cm. The analysis of immune cells showed that the percentage of CD4+T-cells were not altered by liver resection, but the percentage of CD8+T-cell was decreased from 31.7 ± 12.4% to 20.2 ± 10.4% at one week after liver resection (p = 0.006). For immunosuppressor cells, regulatory T-cells were not altered, but myeloid-derived suppressor cells (MDSC) were decreased from 7.75 ± 8.16% to 1.51 ± 1.32% at one month after liver resection (p = 0.022) in 10 of 19 patients with high frequency of MDSC. Furthermore, it was also found that MDSC population was linearly correlated to tumor volume. In conclusion, CD8+ T-cellsand MDSC were altered by liver resection. The percentage of CD8+ T-cellswas decreased by surgery, but the accumulation of MDSC was abrogated.
Neuroactive compounds induce larval settlement in the scleractinian coral Leptastrea purpurea
Authors: Moeller Mareen , Nietzer Samuel , Schupp Peter J. 
Published: February 2019

AbstractSettlement of pelagic coral larvae is commonly induced by chemical cues that originate from biofilms and coralline algae. These natural settlement cues initiate signal pathways leading to attachment and metamorphosis of the coral larva. In order to investigate the settlement process and its natural inducers, it is necessary to gain a better understanding of these signal pathways. At present, the pathways and neurotransmitters involved in this signal transduction are still widely unknown. In this study, we exposed larvae of the brooding coral Leptastrea purpurea to five neuroactive compounds known to be present in cnidarians, and K+ Ions. All compounds were applied at different dilutions and settlement behavior of the larvae was documented over 48 h. Dopamine, glutamic acid and epinephrine significantly induced settlement in the coral larvae. The highest observed metamorphosis response was 54% in 10−5 M dopamine. Serotonin, L-DOPA and K+ ions did not have an influence on settlement behavior in our experiments. Exposing larvae to settlement-inducing neurotransmitters and thus bypassing the initial induction could be utilized in coral aquaculture. The active neurotransmitters should be used to further study the settlement process in L. purpurea in greater detail. Their role and relevance should also be assessed for other coral species as they may represent or reveal a universal inducer for coral settlement.
Upper mesophotic depths in the coral reefs of Eilat, Red Sea, offer suitable refuge grounds for coral settlement
Authors: Kramer Netanel , Eyal Gal , Tamir Raz , Loya Yossi 
Published: February 2019

AbstractDue to increasing frequency of disturbances to shallow reefs, it has been suggested that Mesophotic Coral Ecosystems (MCEs, 30–150 m depth) may serve as a refuge for corals and a source of larvae that can facilitate the recovery of shallow degraded reefs. As such, they have received increased attention in the past decade, yet remained understudied regarding recruitment dynamics. Here we describe coral recruitment dynamics on settlement tiles and their adjacent natural habitats (10 m vs. 50 m depths) in Eilat, over a period of 5.5 years. The tiles were deployed along three sites onto 18 racks (3 at each depth and at each site). Recruitment patterns varied both temporally and spatially, ending up to two-fold higher juvenile density and higher recruitment rates at mesophotic sites. Settlement surface preference changed with depth, favoring exposed surfaces in mesophotic waters and cryptic surfaces in shallow waters. Juvenile assemblages differed between depths and were distinct from adjacent natural habitats. Over half of the recruited genera overlapped between depths. We suggest that Eilat MCEs serve as a larval sink, providing settlement grounds for shallow-reef propagules. In view of their significance, we call for the protection of these unique and distinct deep-reef habitats.
Genome-wide miRNA profiling reinforces the importance of miR-9 in human papillomavirus associated oral and oropharyngeal head and neck cancer
Authors: Božinović Ksenija , Sabol Ivan , Dediol Emil , Milutin Gašperov Nina , Manojlović Spomenka , Vojtechova Zuzana , Tachezy Ruth , Grce Magdalena 
Published: February 2019

AbstractHead and neck cancer is the sixth most common malignancy worldwide, predominantly developing from squamous cell epithelia (HNSCC). The main HNSCC risk factors are tobacco, excessive alcohol use, and the presence of human papillomavirus (HPV). HPV positive (+) cancers are etiologically different from other HNSCC and often show better prognosis. The current knowledge regarding HNSCC miRNA profiles is still incomplete especially in the context of HPV+ cancer. Thus, we analyzed 61 freshly collected primary oral (OSCC) and oropharyngeal (OPSCC) SCC samples. HPV DNA and RNA was found in 21% cases. The Illumina whole-genome small-RNA profiling by next-generation sequencing was done on 22 samples and revealed 7 specific miRNAs to HPV+ OSCC, 77 to HPV+ OPSCC, and additional 3 shared with both; 51 miRNAs were specific to HPV− OPSCC, 62 to HPV− OSCC, and 31 shared with both. The results for 9 miRNAs (miR-9, -21, -29a, -100, -106b, -143 and -145) were assessed by reverse transcription-quantitative polymerase chain reaction on the whole study population. The data was additionally confirmed by reanalyzing publicly available miRNA sequencing Cancer Genome Atlas consortium (TCGA) HNSCC data. Cell signaling pathway analysis revealed differences between HPV+ and HPV− HNSCC. Our findings compared with literature data revealed extensive heterogeneity of miRNA deregulation with only several miRNAs consistently affected, and miR-9 being the most likely HPV related miRNA.
Interleukin 17A promotes diabetic kidney injury
Authors: Ma Jin , Li Yan J. , Chen Xiaochen , Kwan Tony , Chadban Steven J. , Wu Huiling 
Published: February 2019

AbstractThe role of the pro-inflammatory cytokine IL-17 in the pathogenesis of numerous inflammatory disorders is well-documented, but conflicting results are reported for its role in diabetic nephropathy. Here we examined the role of IL-17 signalling in a model of streptozotocin-induced diabetic nephropathy through IL-17 knockout mice, administration of neutralising monoclonal anti-IL-17 antibody and in vitro examination of gene expression of renal tubular cells and podocytes under high glucose conditions with or without recombinant IL-17. IL-17 deficient mice were protected against progression of diabetic nephropathy, exhibiting reduced albuminuria, glomerular damage, macrophage accumulation and renal fibrosis at 12 weeks and 24 weeks. Administration of anti-IL-17 monoclonal antibody to diabetic wild-type mice was similarly protective. IL-17 deficiency also attenuated up-regulation of pro-inflammatory and pro-fibrotic genes including IL-6, TNF-α, CCL2, CXCL10 and TGF-β in diabetic kidneys. In vitro co-stimulation with recombinant IL-17 and high glucose were synergistic in increasing the expression of pro-inflammatory genes in both cultured renal tubular cells and podocytes. We conclude that absence of IL-17 signalling is protective against streptozotocin-induced diabetic nephropathy, thus implying a pro-inflammatory role of IL-17 in its pathogenesis. Targeting the IL-17 axis may represent a novel therapeutic approach in the treatment of this disorder.
Sulphadoxine-pyrimethamine plus azithromycin may improve birth outcomes through impacts on inflammation and placental angiogenesis independent of malarial infection
Authors: Unger Holger W. , Hansa Annjaleen P. , Buffet Christelle , Hasang Wina , Teo Andrew , Randall Louise , Ome-Kaius Maria , Karl Stephan , Anuan Ayen A. , Beeson James G. , Mueller Ivo , Stock Sarah J. , Rogerson Stephen J. 
Published: February 2019

AbstractIntermittent preventive treatment with sulphadoxine-pyrimethamine (SP) and SP plus azithromycin (SPAZ) reduces low birthweight (<2,500 g) in women without malarial and reproductive tract infections. This study investigates the impact of SPAZ on associations between plasma biomarkers of inflammation and angiogenesis and adverse pregnancy outcomes in 2,012 Papua New Guinean women. Concentrations of C-reactive protein (CRP), α-1-acid glycoprotein (AGP), soluble endoglin (sEng), soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF) were measured at enrolment and delivery in a trial comparing SPAZ to SP plus chloroquine (SPCQ). At antenatal enrolment higher CRP (adjusted odds ratio 1.52; 95% confidence interval [CI] 1.03–2.25), sEng (4.35; 1.77, 10.7) and sFlt1 (2.21; 1.09, 4.48) were associated with preterm birth, and higher sEng with low birthweight (1.39; 1.11,3.37), in SPCQ recipients only. Increased enrolment sFlt1:PlGF ratios associated with LBW in all women (1.46; 1.11, 1.90). At delivery, higher AGP levels were strongly associated with low birthweight, preterm birth and small-for-gestational age babies in the SPCQ arm only. Restricting analyses to women without malaria infection did not materially alter these relationships. Women receiving SPAZ had lower delivery AGP and CRP levels (p < 0.001). SPAZ may protect against adverse pregnancy outcomes by reducing inflammation and preventing its deleterious consequences, including dysregulation of placental angiogenesis, in women with and without malarial infection.
Metabolomic and lipidomic plasma profile changes in human participants ascending to Everest Base Camp
Authors: O’Brien Katie A. , Atkinson R. Andrew , Richardson Larissa , Koulman Albert , Murray Andrew J. , Harridge Stephen D. R. , Martin Daniel S. , Levett Denny Z. H. , Mitchell Kay , Mythen Monty G. , Montgomery Hugh E. , Grocott Michael P. W. , Griffin Julian L. , Edwards Lindsay M. 
Published: February 2019

AbstractAt high altitude oxygen delivery to the tissues is impaired leading to oxygen insufficiency (hypoxia). Acclimatisation requires adjustment to tissue metabolism, the details of which remain incompletely understood. Here, metabolic responses to progressive environmental hypoxia were assessed through metabolomic and lipidomic profiling of human plasma taken from 198 human participants before and during an ascent to Everest Base Camp (5,300 m). Aqueous and lipid fractions of plasma were separated and analysed using proton (1H)-nuclear magnetic resonance spectroscopy and direct infusion mass spectrometry, respectively. Bayesian robust hierarchical regression revealed decreasing isoleucine with ascent alongside increasing lactate and decreasing glucose, which may point towards increased glycolytic rate. Changes in the lipid profile with ascent included a decrease in triglycerides (48–50 carbons) associated withde novolipogenesis, alongside increases in circulating levels of the most abundant free fatty acids (palmitic, linoleic and oleic acids). Together, this may be indicative of fat store mobilisation. This study provides the first broad metabolomic account of progressive exposure to environmental hypobaric hypoxia in healthy humans. Decreased isoleucine is of particular interest as a potential contributor to muscle catabolism observed with exposure to hypoxia at altitude. Substantial changes in lipid metabolism may represent important metabolic responses to sub-acute exposure to environmental hypoxia.
Air-like plasmonics with ultralow-refractive-index silica aerogels
Authors: Kim Yeonhong , Baek Seunghwa , Gupta Prince , Kim Changwook , Chang Kiseok , Ryu Sung-Pil , Kang Hansaem , Kim Wook Sung , Myoung Jaemin , Park Wounjhang , Kim Kyoungsik 
Published: February 2019

AbstractThe coupling of the surface plasmon near-field into the sensing medium is key to the sensitivity of surface plasmon-based sensing devices. A low-index dielectric is necessary for the sensing medium to support a highly-penetrating surface plasmon evanescent field that extends well into the dielectric medium. The air-like refractive index, n, of an aerogel substrate provides another dimension for ultralow-index plasmonic devices. In this paper, we experimentally observed an angular surface plasmon resonance dip at 74° with the ultralow-index aerogel substrate, as was expected from theory. We also demonstrated the comparatively high-sensitivity surface plasmon resonance wavelength, λ, while the change in Δλ/Δn with different substrates was studied in detail. A 740 nm-period metal grating was imprinted on aerogel (n = 1.08) and polydimethylsiloxane (PDMS; n = 1.4) substrates. The ultraviolet–visible–near-infrared spectra were observed in the reflection mode on the grating, resulting in sensitivities of 740.2 and 655.9 nm/RIU for the aerogel and PDMS substrates, respectively. Numerical simulations were performed to understand the near-field of the surface plasmon, which demonstrated resonances well correlated with the experimentally observed results. The near-field due to excitation of the surface plasmon polaritons is observed to be more confined and to penetrate deeper into the sensing medium when a low-index substrate is used.
Green tea catechins alleviate autoimmune symptoms and visual impairment in a murine model for human chronic intraocular inflammation by inhibiting Th17-associated pro-inflammatory gene expression
Authors: Li Jian , Yip Yolanda Wong Ying , Ren Jialin , Hui Wing Ki , He Jing Na , Yu Qiu Xiao , Chu Kai On , Ng Tsz Kin , Chan Sun On , Pang Chi Pui , Chu Wai Kit 
Published: February 2019

AbstractAutoimmune uveitis is a sight-threatening disease mainly caused by dysregulation of immunity. We investigated the therapeutic effects of green tea extract (GTE) and its major component, epigallocatechin-3-gallate (EGCG), on a murine model of experimental autoimmune uveoretinitis (EAU). Oral administration of GTE, EGCG, dexamethasone, or water, which started 5 days before the induction, was fed every two days to each group. On day 21 post induction, the eyes were examined by confocal scanning laser ophthalmoscopy, optical coherence tomography (OCT), fundus fluorescein angiography (FFA) and electroretinography (ERG) prior to sacrificing the animals for histological assessments and gene expression studies. Retinal-choroidal thicknesses (RCT) and major retinal vessel diameter were measured on OCT sections and FFA images, respectively. Comparing to water-treated EAU animals, GTE attenuated uveitis clinical manifestations, RCT increase (1.100 ± 0.013 times vs 1.005 ± 0.012 times,P < 0.001), retinal vessel dilation (308.9 ± 6.189 units vs 240.8 units,P < 0.001), ERG amplitudes attenuation, histopathological ocular damages, and splenomegaly in EAU mice. The therapeutic effects of GTE were dose dependent and were comparable to dexamethasone. EGCG, a major active constituent of GTE, partially alleviated uveitic phenotypes including recovering visual function. Th-17 associated pro-inflammatory gene [interleukin 1 beta (IL-1β),IL-6,IL-17A, and tumor necrosis factor alpha(TNF-α)] expressions were down regulated by GTE and EGCG treatments, which showed no detectable morphological defects in liver and kidney in non-induced and EAU mice. Our findings suggest that GTE consumption can serve as a potent therapeutic agent as well as a food supplement for developing alternative treatments against autoimmune uveitis.