bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oncologic outcomes of surgical treatment for T3 glottic laryngeal squamous cell carcinoma
Authors: Zhou Jian , Zhou Liang , Tao Lei , Zhang Ming , Wu Haitao , Chen Xiaoling , Li Xiaoming , Li Cai , Gong Hongli 
Published: March 2018

AbstractBackgroundThe purpose of this study was to assess the clinical results of patients with T3 glottic laryngeal carcinoma treated with total laryngectomy or partial laryngectomy.MethodsWe conducted a retrospectively review of 307 patients with T3 glottic laryngeal squamous cell carcinoma (SCC).ResultsThe 5‐year cancer‐specific survival (CSS) rate was 71.5% and the overall survival (OS) rate was 70.6%. For patients who underwent total laryngectomy, the 5‐year disease‐free survival (DFS) rate was 59.8%, and the CSS rate was 67.9%. For partial laryngectomy, the 5‐year DFS rate was 64.8%, and the CSS rate was 78.0%. High lymph node and stage status are predictors of mortality for these patients. No difference was found in DFS and CSS rates between patients with negative margins and those with positive margins after postoperative radiotherapy and chemotherapy.ConclusionPostoperative radiotherapy and chemotherapy are effective treatments for patients with T3 glottic laryngeal carcinoma, especially with a positive margin. For selected patients with T3 glottic laryngeal carcinoma, partial laryngectomy is a good choice because it can achieve satisfactory oncologic results while preserving laryngeal function.
Relocating cued goals induces population remapping in CA1 related to memory performance in a two‐platform water task in rats
Authors: Lee Justin Quinn , LeDuke Deryn O. , Chua Kate , McDonald Robert J. , Sutherland Robert J. 
Published: March 2018

AbstractThe activity of CA1 neurons in the rodent hippocampus represents multiple aspects of learning episodes, including cue and place information. Previous reports on cue and place representation in CA1 have examined activity in single neurons and population recordings during free exploration of an environment or when actions are directed to either cue or place aspects of memory tasks. To better understand cue and place memory representation in CA1, and how these interact during goal‐directed navigation, we investigated population activity in CA1 during memory encoding and retrieval in a novel water task with two visibly distinct platforms, using mRNA for immediate early genes Arc and Homer1a as markers of neural activity. After training, relocating cues to new places induces an extensive, perhaps global, remapping of the memory code that is accompanied by altered navigation and rapid learning of new cue‐place information. In addition, we have found a significant relationship between the extent of reactivation and overall cue choice accuracy. These findings demonstrate an important relationship between population remapping in CA1 and memory‐guided behavior.
Stakeholder perspectives regarding transfer of free maternity services to National Health Insurance Fund in Kenya: Implications for universal health coverage
Authors: Abuya Timothy , Obare Francis , Matanda Dennis , Dennis Mardieh L. , Bellows Ben 
Published: March 2018

SummaryBackgroundKenya is developing strategies to finance health care through prepayment to achieve universal health coverage (UHC). Plans to transfer free maternity services (FMS) from the Ministry of Health to the National Health Insurance Fund (NHIF) are a step towards UHC. We examined views of health workers and women regarding the transition of FMS to NHIF to inform the process.MethodsIn‐depth interviews among 14 facility‐level managers and providers, 11 county‐level managers, and 21 focus group discussions with women who gave birth before and after the introduction of FMS. Data were analyzed thematically.ResultsThe transfer is a mechanism of achieving UHC, eliminating dependency on free services, and encouraging people to take responsibility of their health. However, skepticism regarding the efficiency of NHIF may limit support. Diverse and robust systems were recommended for enrollment of clients while standardization of services through accreditation and quality assurance linked to performance‐based reimbursement would improve greater predictability in the payment schedule and better coverage of referrals and complications.ConclusionTransitioning FMS to NHIF provides an opportunity for the Ministry of Health to sharpen its role as policymaker and develop a comprehensive health care financing strategy for the country towards achieving UHC.
Hospitalization among migrants in Italy: Access to health care as an opportunity for integration and inclusion
Authors: Barsanti Sara 
Published: March 2018

SummaryThis paper analyzes migrant access to health care by comparing hospitalizations of native and immigrant population with respect the Tuscany Region (Italy). In the analyses, a critical gap both for legal and undocumented migrant population is highlighted. Indeed, we found some key differences between the migrant and native populations related to the use of specific hospital services in Tuscany and, indirectly, of community and primary care services. Moreover, especially for undocumented migrants, hospitals seem to be the only point of access to the health‐care system for migrant populations. The results suggest that the Italian health‐care system is unable to ensure an equitable access to health services. In this context, maternity care could be a key point of access to the welfare system that allows participation in the health system not only for mothers but also for all migrant family members.
Exploring governance for a One Health collaboration for leptospirosis prevention and control in Fiji: Stakeholder perceptions, evidence, and processes
Authors: McPherson Anna , Hill Peter S. , Kama Mike , Reid Simon 
Published: March 2018

SUMMARYFiji has a high burden of leptospirosis, with endemic infection and epidemic outbreaks with high mortality, often associated with flooding and cyclones. As a zoonosis, leptospirosis control requires interventions in sectors beyond the usual control of health—in Fiji, the dairy and sugar industries, and water and sanitation and rodent control in communities. This paper presents the findings of qualitative research to inform policy around governance for a One Health multisectoral approach to leptospirosis control.MethodsKey informants from relevant government agencies and industry organizations were interviewed in late 2014, and the interviews analyzed and triangulated with documentary analysis.FindingsThe analysis identified 5 themes: perceptions of the impact of leptospirosis, governance processes, models for collaboration, leptospirosis control, and preferred leadership for leptospirosis management. Data were limited, with poor communication between ministries, and limited awareness of leptospirosis outside outbreaks. Collaboration during outbreaks was positive but not sustained in endemic periods. Mechanism for enhanced collaboration was developed for endemic and outbreak situations.ConclusionThe findings informed a One Health governance approach to leptospirosis, framed within a National Strategic Plan, with a specific National Action Plan for Leptospirosis. The process provides a research based One Health template for application to other zoonotic diseases.
Evaluating spatial variation of suspended sediment rating curves in the middle Yellow River basin, China
Authors: Zhang Shiyan , Chen Dong , Li Fuxing , He Li , Yan Ming , Yan Yunxia 
Published: March 2018

AbstractAbstractThe suspended sediment load in the middle Yellow River basin (YRB) cannot be well predicted by capacity‐based transport formulas because a large fraction of suspended sediment load is composed of wash load. This study evaluated the spatial variations of sediment rating curves (SRCs) in the middle YRB. Both power and linear SRCs were used to fit daily flow and suspended sediment concentration (SSC) historical data at 49 gauging stations throughout the middle YRB. The spatial variation in regression coefficients was investigated, and the relationship between regression coefficients and the physical characteristics of watersheds was discussed. The results indicate that SRC regression coefficients vary with drainage area and basin slope, but their responses to these parameters are remarkably different in watersheds with different underlying surfaces, which indicates the significance of sediment availability, erodibility, and grain size distribution. For power SRCs representing sediment transport in unsaturated flows, the regression coefficients are more closely correlated with the drainage area in loess regions and with the basin slope in rock mountain regions. For linear SRCs representing sediment transport in saturated flows, saturated SSCs vary with coarse (particle size > 0.05 mm) and fine (particle size < 0.01 mm) fractions in suspended sediment. The maximum saturated SSC among the different gauging stations is associated with the optimal grain size composition of suspended sediment, which has been proposed for loess regions in previous studies. This study provides theoretical support for estimating the regression parameters for sediment transport modelling, especially in ungauged basins.
Reactive glia promote development of CD103+CD69+ CD8+ T‐cells through programmed cell death‐ligand 1 (PD‐L1)
Authors: Prasad Sujata , Hu Shuxian , Sheng Wen S. , Chauhan Priyanka , Lokensgard James R. 
Published: March 2018

AbstractIntroductionPrevious work from our laboratory has demonstrated in vivo persistence of CD103+CD69+ brain resident memory CD8+ T‐cells (bTRM) following viral infection, and that the PD‐1: PD‐L1 pathway promotes development of these TRM cells within the brain. Although glial cells express low basal levels of PD‐L1, its expression is upregulated upon IFN‐γ‐treatment, and they have been shown to modulate antiviral T‐cell effector responses through the PD‐1: PD‐L1 pathway.MethodsWe performed flow cytometric analysis of cells from co‐cultures of mixed glia and CD8+ T‐cells obtained from wild type mice to investigate the role of glial cells in the development of bTRM.ResultsIn this study, we show that interactions between reactive glia and anti‐CD3 Ab‐stimulated CD8+ T‐cells promote development of CD103+CD69+ CD8+ T‐cells through engagement of the PD‐1: PD‐L1 pathway. These studies used co‐cultures of primary murine glial cells obtained from WT animals along with CD8+ T‐cells obtained from either WT or PD‐1 KO mice. We found that αCD3 Ab‐stimulated CD8+ T‐cells from WT animals increased expression of CD103 and CD69 when co‐cultured with primary murine glial cells. In contrast, significantly reduced expression of CD103 and CD69 was observed using CD8+ T‐cells from PD‐1 KO mice. We also observed that reactive glia promoted high levels of CD127, a marker of memory precursor effector cells (MPEC), on CD69+ CD8+ T‐cells, which promotes development of TRM cells. Interestingly, results obtained using T‐cells from PD‐1 KO animals showed significantly reduced expression of CD127 on CD69+ CD8+ cells. Additionally, blocking of glial PD‐L1 resulted in decreased expression of CD103, along with reduced CD127 on CD69+ CD8+ T‐cells.ConclusionsTaken together, these results demonstrate a role for activated glia in promoting development of bTRM through the PD‐1: PD‐L1 pathway.
Exploitation of Feedback in Enzyme‐catalysed Reactions
Authors: Bánsági Támás , Taylor Annette F. 
Published: March 2018

AbstractSome cellular systems, such as yeast, bacteria and slime mould, display dynamic behavior including switches and rhythms driven by feedback in enzyme‐catalysed reactions. The mechanisms of these processes have been well investigated and recent attention has turned to generating similar responses in synthetic biocatalytic systems, with a view to creating bioinspired analogues for applications. Here we discuss how feedback arises in the reaction mechanisms of some enzyme‐catalyzed reactions in vitro, the behaviour obtained and the emerging applications. These autocatalytic reactions may provide insights into behaviour in cellular systems as well as new methods for drug delivery, sensing and repair that can be exploited in living systems.
Matrix metalloproteinase‐9 expression of GCTSC in peripheral tissue and central tissue of GCTB
Authors: Chen Liang , Zhang Ji , He Yifeng , Ding Xiao‐Yi 
Published: March 2018

AbstractGiant cell tumor stromal cell (GCTSC) is the tumor cell of giant cell tumor of bone (GCTB). The biomarkers characterization of GCTSC is critical for the selection of GCTB targeting drugs. We believe the main functions of GCTSC in different part of tumor should be different for different environment. Then the biological behavior and molecular biomarkers of GCTSC should be different as well. Based on this idea, we focused on GCTSC which located in central tissue, peripheral tissue and took MMP‐9 as the breakthrough point to carry out research. The results showed MMP‐9 staining grade of GCTSC which located in central tissue was slight, whereas multinucleated giant cell staining grade was high. The peripheral tissue was consisted by almost GCTSC with high MMP‐9 staining degree and mRNA expression. This study also provided clues and inspiration for reducing GCTB recurrence rate after intralesional curettage with MMP‐9 targeted therapy which were aimed at the residual peripheral tissue.
Retracted: Down‐regulated long non‐coding RNA ANRIL restores the learning and memory abilities and rescues hippocampal pyramidal neurons from apoptosis in streptozotocin‐induced diabetic rats via the NF‐κB signaling pathway
Authors: Wen Xin , Han Xin‐Rui , Wang Yong‐Jian , Wang Shan , Shen Min , Zhang Zi‐Feng , Fan Shao‐Hua , Shan Qun , Wang Liang , Li Meng‐Qiu , Hu Bin , Sun Chun‐Hui , Wu Dong‐Mei , Lu Jun , Zheng Yuan‐Lin 
Published: March 2018

AbstractDiabetes often causes learning and memory deficits, which leads to unfavorable behavioral performance. In this study, we investigated the effects of long non‐coding RNA (lncRNA) ANRIL on learning, memory abilities, and hippocampal neuronal apoptosis via the NF‐κB signaling pathway in streptozotocin (STZ)‐induced diabetic rats. After successful establishment of diabetic rat models, the subjects were then assigned into the DM, DM + si‐ANRIL, DM + si‐negative control (si‐NC) groups, as well as an additional normal group. Morris water maze test was employed to assess behavioral performance of rats, followed by the recording of body weight and blood glucose levels. Expressions of ANRIL, NF‐κB signaling pathway‐related, and apoptosis‐related genes were examined by qRT‐PCR and western blotting. Rat hippocampus expression levels of cleaved‐caspase‐3 were determined by immunofluorescence. Cell apoptosis was examined by TUNEL assay. Versus to the normal group, revealed there to be activation of the NF‐κB signaling pathway, decreased weight, increased blood glucose, increased escape latency, reduced residence time, memory impairment, increased cleaved‐caspase‐3 expression, and increased apoptosis were detected in the DM and DM + si‐NC groups. The DM + si‐ANRIL group exhibited inhibited NF‐κB signaling pathway, weight loss, decreased blood glucose, recovered memory, decreased cleaved‐caspase‐3 expression and reduced apoptosis compared to the DM group, with higher weight of rats, lower blood glucose levels, and stronger memory abilities in the DM + si‐ANRIL group. Taken together, these findings indicate that silencing lncRNA ANRIL promotes memory recovery and decreases hippocampal neurons apoptosis in diabetic rats through the inhibition of the NF‐κB signaling pathway.
TMEM16A inhibition impedes capacitation and acquisition of hyperactivated motility in guinea pig sperm
Authors: Cordero‐Martínez Joaquín , Reyes‐Miguel Tania , Rodríguez‐Páez Lorena , Garduño‐Siciliano Leticia , Maldonado‐García Deneb , Roa‐Espitia Ana L. , Hernández‐González Enrique O. 
Published: March 2018

AbstractCa2+‐activated Cl− channels (CaCCs) are anionic channels that regulate many important physiological functions associated with chloride and calcium flux in some somatic cells. The molecular identity of CaCCs was revealed to be TMEM16A and TMEM16B (also known as Anoctamin or ANO1 and ANO2, respectively) in all eukaryotes. A recent study suggests the presence of TMEM16A in human sperm and a relationship with the rhZP‐induced acrosome reaction. However, to the best of our knowledge, little is known about the role of TMEM16A in other spermatic processes such as capacitation or motility. In this study, we evaluated the effects of two TMEM16A antagonists on capacitation, acrosome reaction, and motility in guinea pig sperm; these antagonists were T16Ainh‐A01, belonging to a second generation of potent antagonists of TMEM16A, and niflumic acid (NFA), a well‐known antagonist of TMEM16A (CaCCs). First of all, we confirmed that the absence of Cl− in the capacitation medium changes motility parameters, capacitation, and the progesterone‐induced acrosome reaction. Using a specific antibody, TMEM16A was found as a protein band of ∼120 kDa, which localization was in the apical crest of the acrosome and the middle piece of the flagellum. Inhibition of TMEM16A by T16Ainh‐A01 affected sperm physiology by reducing capacitation, blocking the progesterone‐induced acrosome reaction under optimal capacitation conditions, inhibiting progressive motility, and the acquisition of hyperactivated motility, diminishing [Ca2+]i, and increasing [Cl−]i. These changes in sperm kinematic parameters provide new evidence of the important role played by TMEM16A in the production of sperm capable of fertilizing oocytes.
RETRACTED: Phytosomal‐curcumin antagonizes cell growth and migration, induced by thrombin through AMP‐Kinase in breast cancer
Authors: Hashemzehi Milad , Behnam‐Rassouli Reihane , Hassanian Seyed Mahdi , Moradi‐Binabaj Maryam , Moradi‐Marjaneh Reyhaneh , Rahmani Farzad , Fiuji Hamid , Jamili Mahdi , Mirahmadi Mahdi , Boromand Nadia , Piran Mehran , Jafari Mohieddin , Sahebkar Amirhossein , Avan Amir , Khazaei Majid 
Published: March 2018

AbstractHere we explored the antitumor‐activity of novel‐formulated‐form of curcumin (phytosomal‐encapsulated‐curcumin) or in combination with 5‐FU in breast cancer. The antiproliferative activity was assessed in 2D and 3‐dimensional cell‐culture‐model. The migratory‐behaviors of the cells were determined by migration assay. The expression levels of CyclinD1,GSK3a/b, P‐AMPK, MMP9, and E‐cadherin were studied by qRT‐PCR and/or Western blotting. The anti‐inflammatory of nano‐curcumin was assessed, while antioxidant activity was evaluated by malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and total thiols (T‐SH). To understand dynamic behavior of genes, we reconstructed a Boolean network, while the robustness of this model was evaluated by Hamming distance. phytosomal‐curcumin suppressed cell‐growth followed by tumor‐shrinkage in 3D model through perturbation of AMP‐activated protein kinase. Curcumin reduced the invasiveness of MCF‐7 through perturbation of E‐cadherin. Moreover, phytosomal‐curcumin inhibited the tumor growth in xerograph model. Histological staining of tumor tissues revealed vascular disruption and RBC extravasation, necrosis, tumor stroma, and inflammation. Co‐treatment of curcumin and 5‐FU reduced the lipid‐peroxidation and increased MDA/SOD level. Of note, curcumin reduced cyclinD‐expression in breast cancer cell treated with thrombin, and activates AMPK in a time‐dependent manner. Also suppression of AMPK abrogated inhibitory effect of phytosomal‐curcumin on thrombin‐induced cyclin D1 over‐expression, suggesting that AMPK is essential for anti‐proliferative effect of this agent in breast cancer. Our finding demonstrated that phytosomal‐curcumin antagonizes cell growth and migration, induced by thrombin through AMP‐Kinase in breast cancer, supporting further‐investigations on the therapeutic potential of this novel anticancer agent in treatment of breast cancer.
Genetic effects of the transcription factors‐sal‐like 1 and spalt‐like transcription factor 3 on egg production‐related traits in Chinese Dagu hens
Authors: Zhu Hongyan , Qin Ning , Tyasi Thobela Louis , Jing Yang , Liu Dehui , Yuan Shuguo , Xu Rifu 
Published: March 2018

AbstractTranscription factors (TFs) encoded by SALL1 and SALL3 genes play central roles in the regulation of ovarian development in hens. The present study aimed to examine polymorphisms of these two genes in Chinese Dagu chickens, and to identify the effects of TFs on the laying performance. Among the population, two novel single‐nucleotide polymorphisms (SNPs) were identified by single‐strand conformation polymorphism (SSCP) in the amplicons of the candidate genes. The effect of the SNP (729C > A) in exon 2 of SALL1 gene on egg production at 43, 57, and 66 weeks and EW at 30 and 43 weeks were the most significant in the 360 samples (P < 0.05). Moreover, for the SNP 1014T > A (in exon 2 of SALL3), the TT genotype was significantly correlated with higher egg production and EW (P < 0.05). Furthermore, four combined genotypes were reconstructed based on the two SNPs. The combined genotype TATT was correlated with the highest egg production at 43–66 weeks and with higher EW at 30, 43 weeks (P < 0.05). The polymorphisms of the two TFs studied are potential molecular genetic markers for chicken breeding, which might help in understanding the genetic structure of laying performance and improving these traits directly by marker‐assisted selection (MAS).
Expression of the Sox9, Foxl2, Vasa, and TRPV4 genes in the ovaries and testes of the Morelet's crocodile, Crocodylus moreletii
Authors: Martínez‐Juárez Adriana , López‐Luna Marco A. , Porras‐Gómez Tania J. , Moreno‐Mendoza Norma 
Published: March 2018

AbstractThe Sox9 gene is important for determining sex in vertebrates, as well as for maintaining testis morphology and fertility during adult life. In the same way, Vasa is an important gene for the maintenance of the germinal lineage and has been highly conserved throughout evolution, as it is expressed in germ cells of both vertebrates and invertebrates. In the particular case of crocodiles, the expression of Sox9 during gonadal morphogenesis and in the testes of 3‐month‐old Alligator mississippiensis has been studied. However, it is interesting to carry out studies on other species of crocodiles in relation to their particular mechanism for sex determination influenced by temperature. In this work, we investigated the expression of the Sox9, Vasa, Foxl2, and TRPV4 genes in the ovaries and testes of 5‐year‐old juvenile crocodiles from Crocodylus moreletii. As expected, Sox9 expression was found in males, but surprisingly, it was also found in females. For the first time, the expression of Vasa was reported in spermatogonia, oogonia, and oocytes of 5‐year‐old crocodiles. Foxl2 is important for the development and maintenance of the ovary during adult life in vertebrates; moreover, Foxl2 protein and transcripts are both highly expressed in the ovaries compared to the testes. A possible upstream regulator of the Sox9 gene in reptiles has not yet been discovered; as such, the expression of the TRPV4 ion channel was evaluated. The TRPV4 ion channel was expressed in the cytoplasm of Sertoli and follicular cells and was therefore proposed as a possible regulator of SOX9.