bluuebunny/crossref_metadata_2025_split huggingface dataset:
Celastrol Protects RPE Cells from Oxidative Stress-Induced Cell Death via Activation of Nrf2 Signaling Pathway
Authors: Zhou Yeqi , Zhou Linbin , Zhou Kewen , Zhang Jingyue , Shang Fu , Zhang Xinyu 
Published: April 2019

Purpose:
Oxidative stress to retinal pigment epithelial (RPE) cells and
inflammation are closely related to the pathogenesis of age-related macular
degeneration (AMD). Celastrol is a natural compound isolated from the root of
Tripterygium wilfordii. Celastrol has been shown to have potent anti-inflammatory and
anti-tumor effects in multiple disease models. The objective of this study was to test the
anti-oxidative effects of celastrol in RPE cells and to investigate the underlying
mechanisms.

Methods:
ARPE-19 cells were treated with hydrogen peroxide (H2O2) and menadione
alone or in combination with celastrol. Cell viability and apoptosis were examined by
CCK-8 and TUNEL assay, respectively. The expression of Nrf2 and its target genes,
such as GCLM and HO-1 was determined by Western blotting. The knockdown of Nrf2
was done by transfecting ARPE-19 cells with lentivirus encoding shRNA against Nrf2.
The knockdown efficiency was determined by real-time quantitative PCR and Western
blotting.

Results:
Treatment of ARPE-19 cells with celastrol significantly attenuated the toxic
effects of both H2O2 and menadione. Treatment with celastrol enhanced the expression
of transcription factor Nrf2 and its targets, GCLM and HO-1. Knockdown of Nrf2
expression by shRNA partially abolished the protective effects of celastrol. Chemical
inhibition of glutathione synthesis by L-buthionine-S,R-sulfoximine (BSO) completely
abolished the protective effects of celastrol against H2O2 and menadione-induced
damage. However, chemical inhibition of HO-1 activity by ZnPPIX did not reduce the
protective effects of celastrol.

Conclusion:
This study provides evidence that treatment of RPE cells with celastrol
shows potent protective effects against oxidative insults via activation of Nrf2 signaling
pathway and upregulation of GCLM expression. This finding suggests that celastrol
might be used as a potential therapeutic agent for oxidative stress-related eyes
diseases, such as AMD.
A Robust Real Time Object Detection and Recognition Algorithm for Multiple Objects
Authors: Modwel Garv , Mehra Anu , Rakesh Nitin , Mishra Krishna K. 
Published: April 2019

Background:
Object detection algorithm scans every frame in the video to detect the objects
present which is time consuming. This process becomes undesirable while dealing with real
time system, which needs to act with in a predefined time constraint. To have quick response we
need reliable detection and recognition for objects.

Methods:
To deal with the above problem a hybrid method is being implemented. This hybrid method
combines three important algorithms to reduce scanning task for every frame. Recursive Density Estimation
(RDE) algorithm decides which frame need to be scanned. You Look at Once (YOLO) algorithm
does the detection and recognition in the selected frame. Detected objects are being tracked
through Speed Up Robust Feature (SURF) algorithm to track the objects in subsequent frames.

Results:
Through the experimental study, we demonstrate that hybrid algorithm is more efficient
compared to two different algorithm of same level. The algorithm is having high accuracy and low
time latency (which is necessary for real time processing).

Conclusion:
The hybrid algorithm is able to detect with a minimum accuracy of 97 percent for all
the conducted experiments and time lag experienced is also negligible, which makes it considerably
efficient for real time application.
Evaluation of a Technology-Based Peer-Support Intervention Program for Preventing Postnatal Depression (Part 2): Qualitative Study
Authors: Shorey Shefaly , Ng Esperanza Debby 
Published: April 2019

Background
Social support is known to reduce risks of postnatal depression (PND) and improve maternal emotional well-being. However, the Asian cultural context is often neglected when appraising maternal needs and mothers’ preferences for social support. While many preventive efforts have experimented with technology, professionals, and paraprofessionals in providing social support to mothers in need, most studies determined the effectiveness of their interventions through quantitative measurements of maternal outcomes. Experiences and feedback from both participants and administrators are rarely discussed, especially in an Asian setting.

Objective
The goal of the research was to evaluate the postnatal experiences of Asian mothers at risk of PND and the perceptions of peer volunteers regarding a technology-based peer-support intervention program (PIP).

Methods
A qualitative semistructured interview was conducted with 20 Asian mothers at risk of depression (10 from the control group and 10 from the intervention group) and 19 peer volunteers from a randomized controlled trial. The PIP included weekly correspondence between peer volunteers and mothers through any telecommunication means over 4 weeks. All interviews were approximately 30 to 60 minutes long, audiotaped, transcribed verbatim, and analyzed using thematic analysis. Study findings were reported according to the Consolidated Standards of Reporting Trials checklist.

Results
Two overarching themes comprising five subthemes were generated: postnatal experience (a bouncy ride, a way forward) and evaluation of the PIP (valuable, flexible, and supportive program; building blocks of a good relationship; and lessons learned and the road ahead). Mothers from both the control and interventions groups were generally satisfied with hospital care and the support received from family. They also shared similar breastfeeding challenges and needs for more informed decisions and follow-up support from the hospital. However, mothers who received the PIP tended to have more positive outlooks of their birth experiences. Overall, peer volunteers and mothers involved in the PIP found the PIP useful and expressed satisfaction with the program’s flexibility. They also shared their personal takeaways, the qualities of their friendships, and the need for extended correspondence time and recommended outreach to non–at-risk mothers.

Conclusions
The positive endorsement of the PIP by peer volunteers and mothers suggests the success of the PIP in maintaining positive maternal emotional well-being during the postpartum period. With the help of technology, hospitals can easily provide additional peer support to at-risk mothers in addition to existing standard care offered to these mothers.

Trial Registration
ISRCTN Registry ISRCTN14864807; http://www.isrctn.com/ISRCTN14864807

International Registered Report Identifier (IRRID)
RR2-10.2196/resprot.9416
AN ANALYSIS OF TEACHERS’ PEDAGOGICAL COMPETENCE IN LESSON STUDY OF MGMP SMP MAJALENGKA
Authors: Emiliasari Raynesa Noor 
Published: April 2019

This research reports an in-depth view of teachers’ pedagogical competence as they participated in the Lesson Study activities. In order to collect the data, observations, notes, videotaping and interviews were used to get comprehensive data in the field. It was revealed that there was a difference between senior and junior teachers in their pedagogical competence. Senior teachers had a better pedagogical competence in terms of: classroom management, understanding the students’ characteristics, curriculum development, lesson plan, and teachers’ talk. Meanwhile, junior teachers were good at: the use of ICT and the development of media in teaching. It was also discovered that both senior and junior teachers were still less in mastering the theory of basic principles in teaching and developing students’ potential. There was no reflective action in teaching and teachers’ less understanding in classroom action research were other factors that affect teachers’ pedagogical competence. It is suggested that in order to improve the quality of teaching, teachers’ pedagogical competence is very important to be improved. Keywords: Teachers’ Pedagogical Competence, Lesson Study
NON-CONTACT THERMOMETER BERBASIS INFRA MERAH
Authors: Safitri Meilia , Dinata Gusti Arya 
Published: April 2019

Pengukuran terhadap suhu tubuh manusia dapat dilakukan dengan menggunakan termometer badan. Jenis termometer badan yang sudah ada saat ini beberapa diantaranya adalah termometer badan analog dan termometer badan digital. Pada umumnya alat-alat tersebut membutuhkan waktu paling cepat selama satu hingga dua menit sampai didapatkan nilai suhu tubuh manusia, sehingga dibutuhkan cara baru untuk mendapatkan nilai suhu tubuh dengan waktu lebih singkat tanpa mengorbankan keakuratan. Dalam penelitian ini telah dirancang sebuah alat yang dapat digunakan untuk mengetahui nilai suhu tubuh manusia dengan waktu yang singkat dan menghasilkan nilai pembacaan yang akurat. Menggunakan sensor infra merah dengan seri MLX90614 yang dipadukan dengan Arduino Nano serta tampilan LCD OLED 128x64 merupakan cara yang digunakan untuk membuat termometer tubuh tanpa kontak fisik. Metode penelitian yang digunakan yaitu membandingkan antara alat non-contact thermometer dengan alat pembanding. Alat pembanding ini sebagai acuan untuk mendapatkan nilai presisi dan akurasi yang tinggi. Kedua alat melakukan pengukuran suhu dalam kondisi yang sama.
SYSTEM USABILITY SCALE VS HEURISTIC EVALUATION: A REVIEW
Authors: Ependi Usman , Kurniawan Tri Basuki , Panjaitan Febriyanti 
Published: April 2019

Usability merupakan salah satu bidang ilmu untuk menganalisa atau menguji tingkat kemudahan penggunaan perangkat lunak. Usability atau yang sering dikenal dengan kebergunaan adalah teknik pengujian atau pengukuran aplikasi perangkat lunak yang dilihat dari lima aspek yaitu learnability, efficiency, memorability, errors dan satisfaction. Untuk melakukan analisa atau pengujian usability dapat dilakukan dengan pendekatan heuristic evaluation (HE) dan system usability scale (SUS). Heuristic evaluation (HE) merupakan pengujian dengan cara melibatkan ahli dalam proses pengerjaannya dan system usability scale (SUS) merupakan pengujian dengan cara melibatkan pengguna akhir (end user) dalam proses pengerjaannya. Untuk itu dalam penelitian dilakukan pengkajian antara heuristic evaluation (HE) dan system usability scale (SUS). Dari hasil kajian didapat bahwa heuristic evaluation (HE) dapat dilakukan bersamaan dengan teknik pengujian lain namun membutuhkan biaya yang besar serta proses pengujian yang lebih mudah. Sedangkan system usability scale (SUS) proses pengujian dan perhitungan lebih rumit namun dapat dilakukan dengan jumlah sampel yang sedikit.
pengaruh akupresur titik Nei Guan terhadap mual muntah akibat kemoterapi emetogenik tinggi pada penyandang kanker anak usia sekolah
Authors: Yuliar Triliana Purwadesi , Susanah Susi , Nurhidayah Ikeu 
Published: April 2019

Salah satu pengobatan penyakit kanker pada anak adalah kemoterapi yang memiliki efek samping mual muntah. Mual muntah pada penyandang kanker anak usia sekolah dapat mengakibatkan gangguan nutrisi, emosional, bermain dan fungsi sekolah. Hal ini dapat menurunkan kualitas hidup anak. Salah satu terapi adjuvan yang dapat menurunkan mual muntah setelah pemberian kemoterapi adalah akupresur yang bersifat non invasif dan tidak membahayakan. Akupresur titik Nei guan menggunakan metode penekanan pada pergelangan tangan. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian akupresur Nei Guan terhadap mual muntah kemoterapi emetogenik tinggi pada penyandang kanker anak usia sekolah. Desain penelitian adalah kuasi eksperimen melalui pendekatan pre-post test design study with control dengan single blind. Responden terbagi atas kelompok kontrol dan kelompok intervensi yang masing-masing berjumlah 15 orang. Kedua kelompok mendapatkan antiemetik yang sama. Pengukuran mual dilakukan 3 kali pada pagi dan malam hari menggunakan instrumen Pediatric Nausea Assessment Tools (PeNAT), sedangkan interval muntah didokumentasikan setiap muntah dan retching. Data dianalisis dengan uji Mann Whitney dan uji t independent. Hasil analisis pada kelompok kontrol menunjukkan rerata nilai mual cenderung mengalami kenaikan dan interval muntah yang lebih cepat dibandingkan dengan kelompok intervensi. Hasil kesimpulannya adalah secara klinis terdapat pengaruh akupresur titik Nei Guan terhadap mual muntah akibat kemoterapi emetogenik tinggi pada penyandang kanker anak usia sekolah meskipun secara statistik belum bermakna (nilai p>0,05). Perawat diharapkan dapat melakukan monitoring mual muntah berkelanjutan dan akupresur dapat dipertimbangkan sebagai terapi adjuvan selain pemberian terapi farmakologi untuk menurunkan mual muntah.
GROWTH CHARACTERISTICS OF ORNAMENTAL JUDAS TREE (Cercis siliquastrum L.) SEEDLINGS UNDER DIFFERENT CONCENTRATIONS OF LEAD AND CADMIUM IN IRRIGATION WATER
Authors: Hatamian Mansoure , Nejad Abdolhossein Rezaei , Kafi Mohsen , Souri Mohammad Kazem , Shahbazi Karim 
Published: April 2019

Landscape plantations are significantly water demanding in many parts of the world, particularly in dry regions. Adequate water supply is the main limiting factor behind landscape development, maintenance and beauty in such environments. On the other hand, monitoring the growth response of ornamental and landscape plants to irrigation water containing heavy metals can be useful in management and application of municipal wastewaters to these plantations instead of application in edible vegetable crops production, which is common in many urban areas. In the present study, one year old seedlings of Judas tree (Cercis siliquastrum) were irrigated for two years with water containing 0, 15 or 30 mg L–1 of lead (Pb) in absence or presence of cadmium (0 or 5 mg L–1) in a factorial design, and under greenhouse conditions. Heavy metal treatments had no significant effect on leaf SPAD value; however, plants treated with Pb15Cd0 combination had significantly higher SPAD value than Pb30Cd5 combination that showed the lowest leaf SPAD value. Increasing the lead and cadmium levels of irrigation water increased the number of chlorotic and necrotic leaves of plant than control, whereas it reduced the average leaf area and new shoot growth. The control plants or plants treated with lower level of heavy metals showed the highest leaf area and new shoot growth. Leaf photosynthesis rate was significantly reduced in all heavy metal treated plants than control plants, as the lowest amount was in Pb30Cd5 combination treatment. All heavy metal treatments showed higher leaf soluble carbohydrates and proline content than in control plants. Leaf soluble carbohydrates were highest in Pb30Cd0 and Pb30Cd5 treatments, and leaf proline was highest in Pb30Cd5 and Pb15Cd5 treatments. The results indicate that Judas tree is a relatively tolerant species to high concentrations of Pb and Cd in root medium added through the irrigation water, and long term dual application of these heavy metals can have additive harmful effects on plant growth.
Dynamic viscoelastic coagulometry of blood obtained from healthy chickens
Authors: Rodenbaugh Cassandra I. , Lyon Shane D. , Hanzlicek Andrew S. , Kanda Ian , Payton Mark E. , Rizzi Theresa E. , Holland LaRinda A. , Brandão João 
Published: April 2019

Abstract

OBJECTIVE
To assess feasibility of the use of a dynamic viscoelastic coagulometer on chicken blood and compare coagulation variables for fresh whole blood and sodium citrate–preserved whole blood as well as effects of 3 coagulation activators on blood from chickens.

SAMPLE
Blood samples from 30 hens.

PROCEDURES
Chickens were allowed to rest undisturbed for 1 hour. A blood sample was collected from an ulnar vein; 1.4 mL was analyzed immediately, and 1.8 mL was mixed with sodium citrate and subsequently recalcified and analyzed. A separate coagulation activator (glass beads, kaolin clay, or tissue factor) was in each of the 2 channels of the analyzer. Chickens were allowed a 1-hour rest period, and another blood sample was collected from the contralateral ulnar vein; it was processed in the same manner as for the first sample, except both channels of the analyzer contained the same coagulation activator.

RESULTS
Compared with fresh samples, citrated samples had higher values for activated clotting time and platelet function and lower clotting rates. Intra-assay coefficients of variation of coagulation profiles for citrated samples were markedly greater than the limit of 10%, whereas values for fresh samples were close to or < 10%.

CONCLUSIONS AND CLINICAL RELEVANCE
Results suggested that use of a dynamic viscoelastic coagulometer on chicken blood was feasible and that analysis of fresh whole blood from healthy chickens provided results with less variability than did analysis of citrated blood. Samples preserved with sodium citrate were associated with significant relative hypocoagulability, compared with results for fresh blood.
Biomechanical comparison of four prosthetic ligament repair techniques for tarsal medial collateral ligament injury in dogs
Authors: Martin Yoanna , Johnson Matthew D. , Travers Collin J. , Colee James , McConkey Marina J. , Banks Scott A. 
Published: April 2019

Abstract

OBJECTIVE
To compare joint stability and ultimate strength among 4 prosthetic ligament constructs for repair of tarsal medial collateral ligament (MCL) injury in dogs.

SAMPLE
13 canine cadavers (26 hind limbs).

PROCEDURES
Each limb was stripped of all soft tissues except those associated with the tarsal joint and assigned to 1 of 4 prosthetic ligament constructs. The AN construct consisted of 3 bone anchors connected with monofilament nylon suture. The AU construct consisted of low-profile suture anchors connected with multifilament ultrahigh-molecular-weight polyethylene (UHMWPE) suture. The TN and TU constructs involved the creation of 3 bone tunnels and use of nylon or UHMWPE suture, respectively. Each limb underwent biomechanical testing before and after MCL transection and before and after cyclic range-of-motion testing following completion of the assigned construct. Tarsal joint stability (extent of laxity) was assessed with the joint in each of 3 positions (75°, 135°, and 165°). After completion of biomechanical testing, each limb was tested to failure to determine the ultimate strength of the construct.

RESULTS
Relative to intact tarsal joints, joint laxity was significantly increased following completion of all 4 constructs. Construct type was not associated with the magnitude of change in joint laxity. Ultimate strength was greatest for the UHMWPE-suture constructs.

CONCLUSIONS AND CLINICAL RELEVANCE
Results indicated that all 4 constructs effectively stabilized MCL-deficient tarsal joints. Implants used for the TU, TN, and AU constructs had a lower profile than those used for the AN construct, which may be clinically advantageous. In vivo studies are warranted.
Use of three-dimensional time-of-flight magnetic resonance angiography at 1.5 Tesla to evaluate the intracranial arteries of 39 dogs with idiopathic epilepsy
Authors: Ishikawa Chieko , Ito Daisuke , Tanaka Natsumi , Kitagawa Masato 
Published: April 2019

Abstract

OBJECTIVE
To assess visualization of the intracranial arteries and internal carotid artery (ICA) on 3-D time-of-flight (TOF) magnetic resonance angiography (MRA) images obtained at 1.5 T and to investigate factors that affect the image quality of those arteries in dogs.

ANIMALS
39 dogs with idiopathic epilepsy.

PROCEDURES
Each dog underwent 3-D TOF MRA, and 5 pairs of intracranial arteries, the basilar artery, and both ICAs were evaluated. Each artery was assigned an image-quality score on a scale of 0 to 3, where 0 = poor and 3 = excellent. Multivariable regression analysis was used to assess whether age, body weight (BW), serum total cholesterol concentration, intracranial volume (ICV), and mean arterial pressure were significantly associated with the image quality of each vessel.

RESULTS
In all dogs, the image-quality score was 2 or 3 for the proximal middle cerebral arteries, basilar artery, and caudal aspect of the caudal communicating arteries. In some dogs, the rostral cerebellar arteries, rostral aspect of the caudal communicating arteries, and middle and rostral aspects of the ICA were poorly visualized. For various arteries, image quality was negatively associated with age and positively associated with BW and ICV.

CONCLUSIONS AND CLINICAL RELEVANCE
Results indicated that 3-D TOF MRA images obtained at 1.5 T did not consistently and clearly delineate the ICA and narrow or peripheral intracranial arteries of dogs; therefore, careful attention is required when such images are assessed. Patient age, BW, and ICV can also affect the image quality of some intracranial arteries on 3-D TOF MRA images. (Am J Vet Res 2019;80:480–489)
Controlling Power Abuse in Public Policy Cycle
Authors: Hai Do Phu 
Published: April 2019

Abstract: Studying the control of power abuse in the public policy cycle is newly an appropriate approach used in the policy science. It is a dynamic examine of the control of political power abuse, so this approach helps to reveal the political-social relation in the control of power abuse. The research article started with an analysis of the role of actors related in the political system working in the public policy cycle. Apparently, it clarified the mechanism of controlling power abuse over core subjects in public policy cycle such as controlling power over the Communist Party of Viet Nam with the participation of the Fatherland Front and its member organizations in public policy planning and public policy evaluation as well as the control of the state power with its key role in the formulation and implementation of public policy in Vietnam.Keywords: Political power, control of power abuse, publiy.
Keywords: Power, control of power abuse, public policy.
Formulation development and in-vitro characterisation of ethosomes for the enhanced transdermal delivery of clotrimazole
Authors: Vidya Viswanad  , Anju PG  , Gopika S Kumar  , Sivapriya G Nair  
Published: April 2019

The target of the study was the development and in vitro characterisation of an effective ethosomal formulation of clotrimazole and to the performance of the formulation was investigated under different preparation conditions. The EE and from 9 formulations, the optimisation was done on the basis of its results which considering the concentration of lecithin and ethanol as the two factors and EE and in vitro release as their responses. The optimization was done on the basis of its results which considering the concentration of lecithin and ethanol as the two factors and EE and in vitro release as their responses. F6 formulation gained a very good release of drug and highest entrapment efficiency of 93.203%. The results of permeation flux were obtained in the order of MLVs > ESUVs > SSUVs > LUVs. This study gives an idea about the vesicles structure and the size, the technique used, the drug EE and the permeation rate. They were taking the above factors into consideration results in improving clinical effectiveness in transdermal delivery of an ethosomal formulation of clotrimazole.