bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retracted: microRNA‐203 promotes proliferation, differentiation, and migration of osteoblasts by upregulation of Msh homeobox 2
Authors: Liu Haochuan , Chen Bing , Li Yi 
Published: March 2019

AbstractDespite the improvements in fracture healing, about 10% of patients undergo abnormal healing. As a tumor suppressor, upregulation of microRNA (miR)‐203 has been observed in osteogenic differentiation. Herein, we aimed to explore the functional role of miR‐203 in osteoblasts as well as the underlying mechanisms. The expression of miR‐203 in MC3T3‐E1 cells that underwent osteogenic differentiation was determined by quantitative reverse transcription PCR (qRT‐PCR). The effects of aberrantly expressed miR‐203 on cell viability, migration, and expressions of proteins associated with proliferation, migration, and osteogenic differentiation were measured by using a Cell Counting Kit‐8 assay, Transwell cell migration assay, and western blot/qRT‐PCR, respectively. The possible downstream factor of miR‐203 was subsequently studied. Finally, involvements of the mitogen‐activated protein kinase (MAPK)/activator of transcription (STAT) pathways were assessed by western blot. We found that the miR‐203 level was increased in osteogenic differentiation of MC3T3‐E1 cells with increasing duration time (28th day, p < 0.001). After cell transfection, we interestingly found that miR‐203 overexpression could increase cell viability (p < 0.05), promote proliferation, migration (p < 0.05), and osteogenic differentiation, and upregulate Msh homeobox 2 (Msx2) expression. Furthermore, Msx2 knockdown was proved to abrogate the effects of miR‐203 overexpression on MC3T3‐E1 cells. Finally, phosphorylated levels of key kinases in the MAPK/STAT pathways were increased by miR‐203 overexpression via upregulating Msx2 expression. In conclusion, miR‐203 overexpression promoted proliferation, migration, and osteogenic differentiation of MC3T3‐E1 cells through upregulating Msx2 along with activation of the MAPK/STAT pathways.
Scavenger receptor Class B type I as a potential risk stratification biomarker and therapeutic target in cardiovascular disease
Authors: Sahebi Reza , Hassanian Seyed Mahdi , Ghayour‐Mobarhan Majid , Farrokhi Effat , Rezayi Majid , Samadi Sara , Bahramian Shabbou , Ferns Gordon A. , Avan Amir 
Published: March 2019

AbstractCardiovascular disease (CVD) is the leading cause of mortality globally. There are few useful markers available for CVD risk stratification that has proven clinical utility. Scavenger receptor B type I (SR‐BI) is a cell surface protein that plays a major role in cholesterol homeostasis through its interaction with high‐density lipoprotein‐cholesterol (HDL‐C) esters (CE). HDL delivers CE to the liver through selective uptake by the SR‐BI. SR‐BI also regulates the inflammatory response. It has been shown that SR‐BI overexpression has beneficial, protective effects in atherogenesis, and there is considerable interest in developing antiatherogenic strategies that involve SR‐BI‐mediated increases in reverse cholesterol transport through HDL and/or low‐density lipoprotein. Further investigations are essential to explore the clinical utility of this approach. Moreover, there is growing evidence showing associations between genetic variants with modulation of SR‐BI function that may, thereby, increase CVD risk. The aim of the current review was to provide an overview of the possible molecular mechanisms by which SR‐BI may affect CVD risk, and the clinical implications of this, with particular emphasis on preclinical studies on genetic changes of SR‐BI and CVD risk.
A direct comparison of four high‐risk human papillomavirus tests versus the cobas test: Detecting CIN2+ in low‐resource settings
Authors: Xue Peng , Gao Li‐Li , Yin Jian , Han Li‐Li , Zhao Jing , Li Li , Seery Samuel , Han Xue‐Yan , Li Ting‐Yuan , Jiang Yu , Chen Wen , Shen Jie 
Published: March 2019

AbstractLow‐cost, accurate high‐risk human papillomavirus (HR‐HPV) tests are needed for cervical cancer screening in limited‐resource settings. More than 200 cervical cytological specimens from hospital patients were collected and analyzed for a real‐world study. We evaluated the analytical and clinical performance of four widely used HR‐HPV test (Tellgen, Hybribio, Liferiver, and Sansure) based on real‐time polymerase chain reaction technology platforms, compared with the cobas test. Cervical intraepithelial neoplasia grade 2 or worse lesions (CIN2+) were set as the disease endpoint, and all the five HPV tests were performed with equal sensitivity (McNemar's test; P = 0.971) and specificity (McNemar's test; P = 0.953). All genotyping using the INNO‐LiPA HPV test showed that HPV‐16, ‐52, and ‐54 were the most common types among CIN2+ cases. Overall, the four HR‐HPV tests analyzed appear to be as effective as the cobas HPV test in both agreement and clinical performance. Therefore, each of these low‐cost HPV test kits could be implemented in limited‐resource settings to accelerate the control of cervical cancer. However, we suggest that there is a need to further standardize and optimize testing around clinical sensitivity and specificity.
Different motivations for knowledge sharing and hiding: The role of motivating work design
Authors: Gagné Marylène , Tian Amy Wei , Soo Christine , Zhang Bo , Ho Khee Seng Benjamin , Hosszu Katrina 
Published: March 2019

SummaryLittle research to date has focused on understanding employee motivation to share and hide knowledge. Using self‐determination theory, we tested the premise that knowledge sharing and hiding might be differentially motivated and that work design characteristics might influence the motivation to share knowledge with colleagues. In a panel survey of Australian knowledge workers and in a Chinese knowledge‐intensive organization, we asked knowledge workers, using time‐lagged designs, about perceptions of work design, motivation to share knowledge, and self‐reported knowledge sharing and hiding behaviors. Results, largely replicated across both samples, indicated that cognitive job demands and job autonomy were positively related to future reports of knowledge‐sharing frequency and usefulness via autonomous motivation to share knowledge. Unexpectedly, task interdependence was positively related to the three forms of knowledge hiding (evasive and rationalized hiding, and playing dumb) via external regulation to share knowledge. Implications for the design of jobs that motivate knowledge sharing and demotivate knowledge hiding are discussed.
Molecular‐weight‐dependent redox cycling of humic substances of paddy soils over successive anoxic and oxic alternations
Authors: Tan Wenbing , Wang Guoan , Zhao Xinyu , Dang Qiuling , Li Renfei , Xi Beidou , Jiang Jie , Zhang Hui , Li Dan , Cui Dongyu , Jia Yufu 
Published: March 2019

AbstractHumic substances (HSs) are critical to regulating methane cycling in temporary anoxic systems because their redox‐active functional groups can sustainably shuttle electrons from microorganisms to oxygen (O2) over successive anoxic and oxic alternations. Whether the relative amount of these redox‐active functional groups involved in redox cycles is associated with the overall chemical structures of HS still remains poorly understood. In this study, variations in the reducing capacities (RCs) of HSs extracted from paddy soils were monitored over consecutive cycles of reduction by iron‐reducing microorganisms and oxidation by O2. Our results show that about 10–40% of the microbially reduced redox‐active moieties within HS, which were reoxidised by O2, cannot restore the same redox state as those before microbial reduction. The restoration extents of RCs of HSs after microbial reduction and O2 reoxidation were greatly negatively correlated with average molecular weight (AMW). The microbially reduced HSs having large AMWs (>1,000 Da) were more kinetically difficult to restore by O2 from a reduced state to an oxidised state than were those having small AMWs (<1,000 Da) possibly because the former, which underwent process of microbial reduction and O2 reoxidation, were more easily sheltered by other parts of HS structures than were the latter. Nevertheless, the unsheltered redox‐active groups that accounted for about 50–85% in HSs can be fully reversible and sustainable to switch between a reduced state and an oxidised state over successive redox cycles. Our results can help to understand the redox dynamics of HSs in biogeochemical cycles in environments.
Green tea–induced epigenetic reactivation of tissue inhibitor of matrix metalloproteinase‐3 suppresses prostate cancer progression through histone‐modifying enzymes
Authors: Deb Gauri , Shankar Eswar , Thakur Vijay S. , Ponsky Lee E. , Bodner Donald R. , Fu Pingfu , Gupta Sanjay 
Published: March 2019

AbstractGreen tea polyphenols (GTPs) and their major constituent, epigallocatechin‐3‐gallate (EGCG), have been reported to demonstrate many interesting biological activities, including anticancer properties. Recent studies on prostate cancer provide strong evidence that epigenetic mechanisms are major players in the regulation of matrix metalloproteinases (MMPs) and their binding partner tissue inhibitor of MMPs (TIMPs) involved in prostate cancer progression. Here we demonstrate that GTP/EGCG mediate epigenetic reactivation of TIMP‐3 that plays a key role in suppressing invasiveness and cancer progression. Treatment of human prostate cancer DUPRO and LNCaP cells with 10 µg/mL GTP and 20 µM EGCG induced TIMP‐3 mRNA and protein expression. This transcriptional activation of TIMP‐3 was associated with the decrease in the expression of both enhancers of zeste homolog 2 (EZH2) and its catalytic product trimethylation of histone H3 at lysine 27 (H3K27me3) repressive marks at the TIMP‐3 promoter with an accompanying increase in histone H3K9/18 acetylation. In addition, GTP/EGCG treatment significantly reduced class I histone deacetylase (HDAC) activity/expression and EZH2 and H3K27me3 levels in prostate cancer cells. EGCG/GTP exposure also reduced MMP‐2/MMP‐9 gelatinolytic activity and abrogated invasion and migration capabilities in these cells. Silencing of EZH2 and class I HDACs strikingly increased the expression of TIMP‐3 independent of DNA methylation. Furthermore, clinical trials performed on patients undergoing prostatectomy consuming 800 mg EGCG (Polyphenon E) up to 6 weeks and grade‐matched controls demonstrate an increase in plasma TIMP‐3 levels. A marked reduction in class I HDACs activity/expression and EZH2 and H3K27me3 levels were noted in GTP‐supplemented prostate tissue. Our findings highlight that TIMP‐3 induction, as a key epigenetic event modulated by green tea in restoring the MMP:TIMP balance suppresses prostate cancer progression.
Simple Modeling to Inform Harvest Strategy Policy for a Data-Moderate Crab Fishery
Authors: Grubert Mark A. , Walters Carl J. , Buckworth Rik C. , Penny Shane S. 
Published: March 2019

Abstract
Attempts to model the giant mud crab Scylla serrata fishery in the Northern Territory (Australia), have often been complex and the results difficult to interpret, leading to divergent estimates of fishing mortality. This has hindered the development of meaningful management policy. Additionally, analyses based on the entire Northern Territory fishery have masked the extreme variation in catches observed along the Gulf of Carpentaria coast. We applied a structurally simple model to visualize the historical patterns in stock size, recruitment, fishing mortality, and fishing mortality at maximum sustainable yield in the western Gulf of Carpentaria mud crab fishery (WGOCMCF) from 1983 to 2017. We also projected future catch and female spawning stock biomass (FSSB) under positive, neutral, and negative recruitment scenarios for three closure periods contained in the fishery harvest strategy (which start in October, if triggered) and compared these with the results of equivalent closures beginning in September. This exercise was undertaken because of known and significant changes in the proportion of fishing effort across different months as well as large variations in the proportion of females harvested each month (with both factors being particularly low in December). These differences were annualized and incorporated into the yearly time step of the model. Predicted catch and FSSB were similar for shorter closure periods (3 or 6 weeks), irrespective of the starting month. However, initiating a 3-month closure in September rather than October could lead to a 16–17% increase in FSSB under negative and average recruitment anomalies, imposing a 9–10% reduction in predicted catch. Based on our experience applying a simple model to the WGOCMCF, we also describe processes and practices that could improve the quality of assessment data for this and other data-moderate crab fisheries.
Gastrostomy tube use in children with cancer
Authors: McGrath Kathleen H. , Hardikar Winita 
Published: March 2019

AbstractChildren with cancer are at risk of malnutrition, which can impair critical childhood processes of growth and development and contribute to poor health outcomes. Enteral nutrition can effectively ameliorate malnutrition or weight loss in children with cancer; however, published nutrition support algorithms contain minimal specific information on gastrostomy tube use, and current literature is limited. Decisions about gastrostomy tube insertion in children with cancer can be challenging. Consideration of gastrostomy tube insertion is only appropriate in children with long‐term dependence on enteral nutrition, particularly when nasogastric tube insertion is predicted or proven to be problematic. Specific indications for patient selection are unclear, and referring clinicians may be unaware of important absolute and relative contraindications. Complications are predominantly minor in nature; however, reported rates are high. Morbidity must be weighed carefully against the need and anticipated duration of enteral nutrition support, and further research in this area is needed.
Thrombopoietin receptor agonist switch in children with persistent and chronic severe immune thrombocytopenia: A retrospective analysis in a large tertiary center
Authors: Suntsova Elena V. , Maschan Alexey A. , Baydildina Dina D. , Kalinina Irina I. , Petrova Uliana N. , Pshonkin Alexey V. , Novichkova Galina A. 
Published: March 2019

AbstractWe retrospectively analyzed sequential therapy with romiplostim and eltrombopag in 23 children with immune thrombocytopenia: switching from romiplostim to eltrombopag (10 patients) or vice versa (13 patients). The median age of patients at enrollment in the study was 5.6 years (2‐15 years). Switching from romiplostim to eltrombopag was effective in eight (80%) patients, whereas switching from eltrombopag to romiplostim was effective in eight (62%) patients. The response rate was similar in patients failing the first thrombopoietin receptor agonist and those who had previous response. To date, all responders continue to maintain platelets over 50 × 109/L at 13‐39 months after switching.
Examination of the prescription of antiepileptic drugs to prenatal and postpartum women in Japan from a health administrative database
Authors: Ishikawa Tomofumi , Obara Taku , Jin Kazutaka , Nishigori Hidekazu , Miyakoda Keiko , Suzuka Masato , Ikeda‐Sakai Yasuko , Akazawa Manabu , Nakasato Nobukazu , Yaegashi Nobuo , Kuriyama Shinichi , Mano Nariyasu 
Published: March 2019

AbstractPurposeTo evaluate the prevalence and patterns of prescriptions of antiepileptic drugs (AEDs) to prenatal and postpartum women in Japan using a large administrative database.MethodsThe dates of pregnancy onset and delivery were estimated using published algorithms and infant birth months. The prevalence of prescribed AEDs, the maximum dose of some AEDs, and the frequency of potential combination therapy with AEDs were evaluated for the 180 days before pregnancy onset, during pregnancy, and at 180‐day postpartum.ResultsIn total, 33 941 pregnant women were eligible for analysis. At least one AED was prescribed to 225 women (66 per 10 000 deliveries) between 180 days before pregnancy and 180‐day postpartum and for 135 women (40 per 10 000 deliveries) during pregnancy. The prevalence of AED prescription declined during the first and second trimesters and increased in the third trimester and postpartum. Valproate was the most frequently prescribed drug, followed by clonazepam, lamotrigine, and carbamazepine. Nine (18.4%) of the 49 women with at least one prescription record of valproate in the first trimester were prescribed more than 600 mg/day of valproate. Concerning potential combination therapy, 40 (12 per 10 000 deliveries) concurrently received two or more AEDs between 180 days before pregnancy and 180‐day postpartum, respectively, 31 (9 per 10 000 deliveries) women received these drugs during pregnancy.ConclusionsVarious AEDs were prescribed to pregnant Japanese women. Women of reproductive age should select the appropriate AED before becoming pregnant, depending on the risk benefit profile.
Electrically conductive and mechanically tough graphene nanocomposite hydrogels with self‐oscillating performance
Authors: Ren Jie , Zhang Aixia , Zhang Lan , Li Yan , Yang Wu 
Published: March 2019

AbstractConventional hydrogels are extremely brittle, fragile and poorly conductive, which limits their applications in a variety of aspects. In this study, we fabricated a novel kind of nanocomposite self‐oscillating hydrogel poly(AA‐co‐Fe(phen)3)/PVA/RGO with high conductivity and good mechanical strength by dispersing reduced graphene oxide (RGO). Due to the synergetic effect of RGO dispersed in the hydrogels or dry gels and Fe metal which is the reduction product of the Fe(phen)3 moiety by RGO, the hydrogels have a high conductivity of 18.2 S m−1 with 0.67 wt% RGO content. The dispersed RGO in the hydrogels combined with the network structure by means of hydrogen bonding, π–π stacking and electrostatic interaction and was demonstrated to enhance the mechanical properties of the hydrogels. The elastic modulus achieves 65.2 kPa (1020% of the tensile strength) and 236.4 kPa (with 70% compression), respectively. In addition, the prepared hydrogels exhibit a self‐oscillating behavior in a Belousov–Zhabotinsky solution free of catalyst. These results can be broadly applied in the future in the development of an autonomous on–off switching, flexible/stretchable, graphene‐based soft electronic device. © 2019 Society of Chemical Industry
Effect of elevated carbon dioxide concentration on growth, productivity and glyphosate response of parthenium weed (Parthenium hysterophorus L.)
Authors: Bajwa Ali A , Wang Haochen , Chauhan Bhagirath S , Adkins Steve W 
Published: March 2019

AbstractBACKGROUNDThe rise in atmospheric CO2 has huge impacts on the biology and management of invasive weed species such as Parthenium hysterophorus. This study evaluated the effect of ambient (400 ppm) and elevated (700 ppm) CO2 concentrations on P. hysterophorus growth, reproductive output and response to glyphosate applied at several doses including the recommended dose (800 g a.e. ha−1).RESULTSThe plants in control treatment (no herbicide) grew taller (41%), produced a larger number of leaves (13%) and flowers (39%), and higher dry biomass (34%) at elevated CO2 as compared to the ambient CO2. Glyphosate caused significant reduction in chlorophyll content of P. hysterophorus plants grown at both CO2 concentrations in a dose‐dependent manner. The percentage herbicide injury was relatively less at elevated CO2 as compared to the ambient CO2 at 7 and 14 days after glyphosate application but it was almost similar at 21 days after application. This shows that elevated CO2 might have slowed the translocation of glyphosate initially, but most plants were killed eventually close to 21 days after application. The survival rate was higher under elevated as compared to the ambient CO2 at recommended and lower doses of glyphosate. There was a negligible difference between the two CO2 concentrations for the plant dry biomass reduction over the control treatment.CONCLUSIONSP. hysterophorus growth and reproductive potential (indicated by number of flowers) improved significantly by CO2 enrichment but there was little effect on the overall efficacy of glyphosate applied to control this species. © 2019 Society of Chemical Industry
Extending the Effectiveness of Electrofishing to Estuarine Habitats: Laboratory and Field Assessments
Authors: Lieschke Jason A. , Dean Jan C. , Pickworth Andrew 
Published: March 2019

AbstractElectrofishing is a widely used and highly effective method of sampling freshwater fishes. However, power limitations currently restrict electrofishing to waterways with ambient electrical conductivities up to 5,000 μS/cm. At these higher conductivities, the electrofishing devices currently available are limited in providing the peak power required for effective electrofishing (i.e., peak power drops below the fish capture threshold). The Grassl Model EL65IIGI1 electrofisher (GEF) is a new, high‐power boat electrofishing system designed to successfully fish waters with electrical conductivities exceeding 5,000 μS/cm, but its electrical output and effective power range for fish capture remain untested. We used tank and field electrical load tests to document the output power capability of the GEF over a wide range of electrical resistances and conductivities. Maximum power output for the low voltage range was 246,500 peak watts, with a maximum current of 1,880 peak amperes. Effective fish capture conductivity for the GEF with standard electrodes was estimated to range from 30 to 35,000 μS/cm. Our results demonstrate that the GEF greatly extends electrofishing capability into estuarine and brackish‐water habitats.
How commuting affects subjective wellbeing
Authors: Clark Ben , Chatterjee Kiron , Martin Adam , Davis Adrian 
Published: March 2019

Abstract
Commuting between home and work is routinely performed by workers and any wellbeing impacts of commuting will consequently affect a large proportion of the population. This paper presents findings from analyses of the impact of commuting (time and mode) on multiple aspects of Subjective Well-Being (SWB), including: satisfaction with life overall and the SWB sub-domains of job satisfaction, satisfaction with leisure time availability and self-reported health. Measures of strain and mental health (GHQ-12) are also examined. Six waves of individual-level panel data from Understanding Society (2009/10 to 2014/15) are analysed, providing a sample of over 26,000 workers living in England. Associations between commuting and SWB are identified, paying particular attention to those arising from individual changes in commuting circumstances over the six waves. It is found that longer commute times are associated with lower job and leisure time satisfaction, increased strain and poorer mental health. The strongest association is found for leisure time satisfaction. Despite these negative associations with the SWB sub-domains, longer commute times were not associated with lower overall life satisfaction (except where individuals persisted with them over all six waves). Workers in England appear to be successful in balancing the negative aspects of commuting against the wider benefits, e.g. access to employment, earnings and housing. Differences amongst selected population sub-groups are also examined. The job satisfaction of younger adults and lower income groups are not found to be negatively associated with longer commute times; longer commute times are more strongly negatively associated with the job satisfaction of women compared to men. With respect to mode of transport, walking to work is associated with increased leisure time satisfaction and reduced strain. The absence of the commute, via working from home, is associated with increased job satisfaction and leisure time satisfaction. Overall, the study indicates that shorter commute times and walkable commutes can contribute to improved SWB—particularly through the release of leisure time. But life satisfaction overall will only be maintained if the benefits of undertaking the commute (earnings and satisfactory housing/employment) are not compromised.