bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic basis of depressive disorders
Authors: Davydova Yu. D. , Enikeeva R. F. , Kazantseva A. V. , Mustafin R. N. , Romanova A. R. , Malykh S. B. , Khusnutdinov E. K. 
Published: July 2019

Depression is a common mental disorder being one of the main causes of disability and mortality worldwide. Despite an intensive research during the past decades, the etiology of depressive disorders (DDs) remains incompletely understood; however, genetic factors are significantly involved in the liability to depression. The present review is focused on the studies based on a candidate gene approach, genome-wide association studies (GWAS) and whole exome sequencing (WES), which previously demonstrated associations between gene polymorphisms and DDs. According to the first approach, DD development is affected by serotonergic (TPH1, TPH2, HTR1A, HTR2A, and SLC6A4), dopaminergic (DRD4, SLC6A3) and noradrenergic (SLC6A2) system genes, and genes of enzymatic degradation (MAOA, COMT). In addition, there is evidence of the involvement of HPA-axis genes (OXTR, AVPR1A, and AVPR1B), sex hormone receptors genes (ESR1, ESR2, and AR), neurotrophin (BDNF) and methylenetetrahydrofolate reductase (MTHFR) genes, neuronal apoptosis (CASP3, BCL-XL, BAX, NPY, APP, and GRIN1) and inflammatory system (TNF, CRP, IL6, IL1B, PSMB4, PSMD9, and STAT3) genes in DD development. The results of the second approach (GWAS and WES) revealed that the PCLO, SIRT1, GNL3, GLT8D1, ITIH3, MTNR1A, BMP5, FHIT, KSR2, PCDH9, and AUTS2 genes predominantly responsible for neurogenesis and cell adhesion are involved in liability to depression. Therefore, the findings discussed suggest that genetic liability to DD is a complex process, which assumes simultaneous functioning of multiple genes including those reported previously, and requires future research in this field.
Evaluation of an Early Language and Literacy Program for Parents of Infants in the NICU
Authors: Shanty Lisa , Dowling Rebecca , Sonnenschein Susan , Hussey-Gardner Brenda 
Published: July 2019

PurposeTo evaluate the effect of a NICU parent education program on parents' early language and literacy practices, and on their confidence interpreting and responding to infant signals.DesignSingle group, pre- and post-test, mixed-methods evaluation design.SampleOne hundred and four parents and other caregivers completed questionnaires before and after the one-hour program. Ten parents participated in follow-up interviews.Main Outcome VariablesBefore and after sessions, participants reported on frequency of their current and intended early language and literacy practices, and their confidence interpreting and responding to infant signals. Participants also reported program satisfaction. Interview participants reported their behavior change one to two weeks later.ResultsThe program significantly increased intention to engage in more early language and literacy practices, and increased parent-reported knowledge of how and when to interact with their infants. The majority of interviewed parents reported engaging in these practices one to two weeks later.
JUAL BELI BITCOIN DI INDODAX.COM. DALAM PERSPEKTIF SYARIAH
Authors: Aufima Zidna 
Published: July 2019

AbstractThe use of bitcoin as a currency or payment instrument is prohibited by Indonesian Bank. However, bitcoin as an object of sell buy in the form of digital assets or intangible goods is traded on indodax.com. The method used is statute approach and conceptual approach. The results of this study can be known that the law of sell buy bitcoin on indodax.com in the perspective of sharia is forbidden because in fiqh, the contract of sell buy bitcoin on indodax.com. include fasid contract because bitcoin contains elements gharar, maysir, syubhat, and dharar as an object of sell buy so as to violate the provisions of sharia. Indonesian Ulema Council should issue National Sharia Board About Bitcoin. Keywords : Sell Buy, Bitcoin, Digital Assets, Indodax.com, The Perspective of Sharia AbstrakPenggunaan bitcoin sebagai mata uang atau alat pembayaran dilarang oleh Bank Indonesia. Namun, bitcoin sebagai obyek jual beli yang berbentuk aset digital atau barang tidak berwujud diperjualbelikan di indodax.com. Metode yang digunakan adalah statute approach dan conceptual approach. Hasil penelitian ini dapat diketahui bahwa hukum jual beli bitcoin di indodax.com dalam perspektif syariah adalah dilarang karena dalam fikih, akad jual beli bitcoin di indodax.com. termasuk akad yang fasid karena bitcoin mengandung unsur gharar, maysir, syubhat, dan dharar sebagai obyek jual beli sehingga melanggar ketentuan syariah. Seharusnya Majelis Ulama Indonesia (MUI) mengeluarkan Fatwa Dewan Syariah Nasional (DSN) tentang Bitcoin. Kata Kunci : Jual Beli, Bitcoin, Aset Digital, Indodax.com, Perspektif Syariah.
Analysis and Prospects of Using Recombinant Vaccinia Virus MVA Strain as a Vector in the Development of the Vaccines against Human and Simian Immunodeficiency Virus Diseases
Authors: Stovba L. F. , Krotkov V. T. , Paveli’ev D. I. , Mel’nikov S. A. , Lebedev V. N. , Borisevich S. V. 
Published: July 2019

The review presents the results of preclinical use of vector vaccines against human immunodeficiency virus (HIV) disease and simian immunodeficiency virus (SIV) disease. Application of antiretroviral therapy exclusively is insufficient for elimination of HIV from patient’s body. This dictates the need for an effective vaccine which will reduce the number of new cases of the disease and reduce the risk of virus transmission. Current practice of medicinal product development showed the effectiveness of heterologous prime-boost regimens for the induction of expressed immune response in laboratory animals. Various vector constructs were used as priming vaccines: DNA vaccines, Bacille Calmette-Guerin vaccine, chimpanzee adenovirus, vesicular stomatitis virus, alphavirus repli-clone. Booster vaccine was represented by recombinant MVA strain. In all vector vaccines, different genes of immunodominant antigens of HIV and SIV agents were inserted. On rhesus macaques, murine, rabbit models, it was demonstrated that deployed vaccination schemes were safe and induced immune response. Because membrane HIV protein is highly variable, strongly glycoziled and subjected to structural changes during receptor binding, it cannot be viewed as a target for induction of virus neutralized antibodies. Therefore, we mainly studied the cell immune response that was presented by poly-functional CD8+ T-cells. However, some recent researches are aimed at such modification of envelope HIV immunogene that would provide for virus neutralizing antibody induction. The study of protective efficiency of the induced immunity in rhesus macaques, immunized with recombinant vectors expressing SIV’ s immunodominant antigens, in case of subsequent inoculation with virulent SIV strain has revealed that all monkeys developed illness. Assuming that the constructions with SIV’ s immunodominant antigens under protective efficiency testing on rhesus macaques imitate AIDS in humans, it seems that vaccines, developed up-to-date, will not be effective for collective immunity formation against AIDS. Therefore, the search for novel combinations of expressed immunodominant antigens for the inclusion into the composition of priming and booster vaccines remains a priority area at present time.
Effect of Bacteriophages and Gentamycine on Morphology and Vesicle Formation of Bacteria Yersinia pestis EV
Authors: Dudina L. G. , Malkova M. A. , Chernyad’ev A. V. , Litvinets S. G. , Byvalov A. A. 
Published: July 2019

Objective was to assess the effect of specific bacteriophages and gentamycine on the morphological-functional properties of bacteria in the vaccine strain Yersinia pestis EV.Materials and methods. The vaccine strain Y. pestis EV, Pokrovskaya bacteriophage and the pseudotuberculous diagnostic bacteriophage were used for the study. The microbial culture was grown on solid and in liquid growth media at 27 °C for 20–24 h. The co-incubation of bacteria and bacteriophage or gentamycine was carried out at 27 °C for 20 minutes or at 37 °C for 2 hours, respectively. Culture preparations were examined by transmission electron microscopy.Results and discussion. The influence of cultivation conditions and various stress factors on the vesicle production by the vaccine strain Y. pestis EV cells was evaluated. The nature and intensity of morphological-functional changes in Y. pestis EV cells in response to the effect of bacteriophages (plague Pokrovskaya and pseudotuberculous bacteriophages) or an antibiotic (gentamycine) were determined. It was established that co-incubation of Y. pestis EV with Pokrovskaya bacteriophage or gentamycine for 20 min leads to the increase in the production of extracellular vesicles and is accompanied by the development of degenerative changes in bacterial cells.
Epidemiological Significance of Hantavirus Infection in Crimea
Authors: Zinich L. S. , Kovalenko I. S. , Pidchenko N. N. , Tikhonov S. N. 
Published: July 2019

Objective of this work was to determine epidemiological significance of Hantaviruses, circulating in Crimea, on the basis of retrospective data and taking into account the results obtained over the monitoring period of 2015–2018.Materials and methods. The study of small mammals and blood sera of donors was carried using enzyme-linked immunosorbent assay.Results and discussion. Held in 1985–1989 studies of hemorrhagic fever with renal syndrome (HFRS) suggested that Hantaviruses, circulating among the voles of the genus Microtus in the territory of Crimea, do not have etiological significance in the structure of HFRS incidence. A 2008 study found the circulation of Hantaviruses of the Tula serotype among Microtus arvalis (obscurus). Studies on the natural focality of Hantavirus infection in Crimea during 2015–2018 showed circulation of Hantaviruses among small mammals: Microtus socialis, Mus musculus, Sylvaemus witherbyi, Crocidura suaveolens, which are not the main reservoirs of pathogenic Hantaviruses. Also, seropositive to hantavirus donors were detected – 0.4 %. Local cases of HFRS infection for the entire observation period were not registered. To confirm the positive findings and determine the epidemiological significance of circulating pathogens, samples with positive for Hantaviruses findings were sent to the reference center for HFRS in 2017. The results of the investigation did not confirm the presence of antibodies to human pathogenic Hantaviruses in the blood sera of donors; in the biological material from mouse-like rodents, the antigen of pathogenic for humans serotypes of Hantavirus was not detected. Thus, in the natural foci of Crimea in 2015–2018, the circulation of Hantaviruses which do not belong to pathogenic for humans serotypes of Hantavirus Puumala, Hantaan, Dobrava was registered. Detection of seropositive donors testifies to natural immunization of the population through circulation of Hantaviruses that are non-pathogenic for humans or Hantaviruses of other serotypes.
Size and Shape of the Human Anterior Cruciate Ligament and the Impact of Sex and Skeletal Growth
Authors: Cone Stephanie G. , Howe Danielle , Fisher Matthew B. 
Published: July 2019

Background:
High rates of anterior cruciate ligament (ACL) injury and surgical reconstruction in both skeletally immature and mature populations have led to many studies investigating the size and shape of the healthy ligament. The purposes of the present study were to compile existing quantitative measurements of the geometry of the ACL, its bundles, and its insertion sites and to describe effects of common covariates such as sex and age.

Methods:
A search of the Web of Science was conducted for studies published from January 1, 1900, to April 11, 2018, describing length, cross-sectional area, volume, orientation, and insertion sites of the ACL. Two reviewers independently screened and reviewed the articles to collect quantitative data for each parameter.

Results:
Quantitative data were collected from 92 articles in this systematic review. In studies of adults, reports of average ACL length, cross-sectional area, and volume ranged from 26 to 38 mm, 30 to 53 mm2, and 854 to 1,858 mm3, respectively. Reported values were commonly found to vary according to sex and skeletal maturity as well as measurement technique.

Conclusions:
Although the geometry of the ACL has been described widely in the literature, quantitative measurements can depend on sex, age, and measurement modality, contributing to variability between studies. As such, care must be taken to account for these factors. The present study condenses measurements describing the geometry of the ACL, its individual bundles, and its insertion sites, accounting for common covariates when possible, to provide a resource to the clinical and scientific communities.

Clinical Relevance:
Quantitative measures of ACL geometry are informative for developing clinical treatments such as ACL reconstruction. Age and sex can impact these parameters.
Asymptomatic Rotator Cuff Tears
Authors: Lawrence Rebekah L. , Moutzouros Vasilios , Bey Michael J. 
Published: July 2019

» Asymptomatic rotator cuff tears (RCTs) are prevalent in the general population; they are positively associated with age and are common in the contralateral shoulder of individuals who are being treated for shoulder pain or a symptomatic RCT.» Asymptomatic RCTs are likely to become symptomatic over time, corresponding with decreased patient-reported function, strength, and range of motion.» Previous studies have largely reported inconsistent findings regarding patient-reported outcomes, strength, range of motion, and kinematics in individuals with asymptomatic RCTs.» Future research would benefit from characterizing any functional alteration that is associated with asymptomatic rotator cuff pathology, including determining whether such alterations are detrimental or compensatory and understanding the mechanism by which an asymptomatic RCT becomes symptomatic.
Carbonic Acid Resistance of Hydroxyapatite-Containing Cement
Authors: Srivastava Aman , Ahmed Ramadan , Shah Subhash 
Published: July 2019

Summary
With the current applications of carbon dioxide (CO2) in oil wells for enhanced-oil-recovery (EOR) and sequestration purposes, the dissolution of CO2 in the formation brine, and the resulting formation of carbonic acid (H2CO3), is a major cause of cement damage. This degradation can lead to noncompliance with the functions of the cement because it changes compressive strength and shear-bond strength (SBS) and the porosity and permeability of cement. It becomes imperative to understand the degradation mechanism of cement and methods to reduce the damage, such as the use of special additives to improve the resistance of cement to an acid attack. Hence, the primary objective of this study is to investigate the effects of hydroxyapatite on cement degradation.
To investigate the effects of hydroxyapatite additive on oil-well-cement performance, two Class H cement slurry formulations [the baseline Class H cement containing silica flour (HS) and the hydroxyapatite-containing cement (HHO), a Class H cement containing 5% hydroxyapatite by weight of cement (BWOC)] were compared after exposure to acidic environments. To evaluate the performance of the formulations, samples were prepared and aged in a high-pressure/high-temperature (HP/HT) autoclave containing 2% brine saturated with mixed gas containing methane (CH4) and CO2. Tests were performed at different temperatures (38 to 221°C), pressures (21 to 63 MPa), and CO2 concentrations (10 to 100%). After aging for 14 days at constant pressure and temperature, the samples were recovered and their bond strength and compressive strength and their porosity and permeability (i.e., overall apparent permeability) were measured and compared with those of unaged samples.
The results demonstrated that adding hydroxyapatite limits carbonation. Baseline samples that did not contain hydroxyapatite carbonated, and consequently their compressive strength, porosity, permeability, and SBS significantly changed after aging, whereas hydroxyapatite-containing samples displayed a limited change in their properties. However, hydroxyapatite-containing samples exhibit high permeability because of the formation of microcracks after exposure to H2CO3 at high temperature (221°C). The formation of microcracks could be attributed to thermal retrogression or other phenomena that cause the expansion of the cement.
This paper sheds light on the application of hydroxyapatite as a cement additive to improve the H2CO3 resistance of oilwell cement. It presents a hydroxyapatite-containing cement formulation that has acceptable slurry properties for field applications and better H2CO3 resistance compared with conventional cement.
The microbiome in benign renal tissue and in renal cell carcinoma
Authors: Heidler Stefan , Lusuardi Lukas , Madersbacher Stephan , Freibauer Christa 
Published: July 2019

Abstract
Background

To describe the renal microbiome and to determine differences of the renal microbiome in healthy and tumour-bearing parenchyma.

Methods

10 biopsies from patients undergoing laparoscopic nephrectomy for renal carcinoma with no history of urinary tract infections within the last 6 months were included in this study. The identification of all microorganisms was done using 16s DNA sequencing. The beta diversity analysis was performed by Bray - Curtis dissimilarity.

Results

In all kidney samples, a plethora of microorganisms was found, with significant differences between benign and malignant renal tissue (p< 0.0001).

Conclusions

There is evidence that healthy kidney tissue as well as renal cell cancer tissue have a specific microbiome, thus opening new perspectives in renal physiology and tumour pathogenesis.
Plasma HER2ECD a promising test for patient prognosis and prediction of response in HER2 positive breast cancer: Results of a randomized study - SAKK 22/99
Authors: Eppenberger-Castori Serenella , Klingbiel Dirk , Ruhstaller Thomas , Rufle Daniel Alexander , Rothgiesser Karin , Pagani Olivia , Thürlimann Beat 
Published: July 2019

Abstract
Background

The HER2 extracellular domain shed in blood (HER2ECD) is reported to rise and fall in parallel with HER2+ breast cancer behavior. In this study, we evaluated the clinical relevance of plasma HER2ECD values in patients with metastatic breast cancer treated in the SAKK22/99 trial comparing trastuzumab monotherapy followed by trastuzumab-chemotherapy combination at progression versus upfront combination therapy.

Methods

Quantitative assessment of plasma HER2ECD was performed in 133 patients at baseline; after 2-24 hours; at 3 weeks; at first response evaluation (8-9 weeks); and at tumor progression. Associations with tumor characteristics, disease course and trial treatment were evaluated.

Results

Baseline HER2ECD levels were stable within 24 hours after the first trastuzumab injection. These plasma values correlated positively with the HER2 gene ratio (rs=0.39, P<0.001) and HER2 protein expression levels (rs=0.36, P<0.001) but not with ER/PR status of the primary tumor. HER2ECD baseline levels were positively associated with the presence of visceral disease (P=0.05) and poor patients’ outcome (Cox-regression: P=0.009). Patients with high baseline levels (>35ng/ml) had the worst overall survival (P=0.03) if treated with upfront combination therapy. Conversely, patients with low HER2ECD baseline values (<15ng/ml) had longer TTP-TChemo when first treated with trastuzumab monotherapy (P=0.02). Monitoring HER2ECD levels during the course of the trial revealed significant time (P=0.001) and time-treatment arm interactions (P=0.0007). Under upfront trastuzumab alone, the HER2ECD levels remained stable until just before disease progression. In patients responding to combination treatment HER2ECD levels decreased to >20%.

Conclusions

Plasma HER2ECD levels in patients with metastatic breast cancer reflect HER2 disease status. This robust biomarker might help identifying patients without visceral disease profiting from a sequential treatment’s modality. Monitoring HER2ECD levels during trastuzumab monotherapy could help defining the optimal time to introduce chemotherapy.
Influence of pre-operative oral carbohydrate loading vs. standard fasting procedure on tumor proliferation and clinical outcome in breast cancer patients — a randomized trial
Authors: Lende Tone Hoel , Austdal Marie , Varhaugvik Anne Elin , Skaland Ivar , Gudlaugsson Einar , Kvaløy Jan Terje , Akslen Lars , Søiland Håvard , Janssen Emiel , Baak Jan PA 
Published: July 2019

Abstract
Background

The influence of carbohydrates in breast cancer is conflicting.

Objective

To determine whether preoperative per-oral carbohydrate load influences proliferation in breast tumors.

Design

Randomized controlled trial.

Setting

University hospital with primary and secondary care functions in South-West Norway.

Patients

A population-based cohort of 61 patients with operable breast cancer.

Intervention

Per-oral carbohydrate load (preOp™) 18 and 2-4 hours before surgery (n=26) or standard pre-operative fasting procedure with free consume of tap water (n=35).

Measurements

Primary outcome was post-operative tumor proliferation measured as mitotic activity index (MAI). Secondary outcomes were changes in serum insulin, insulin-c-peptide, glucose, IGF-1 and IGFBP3. Other secondary outcomes were patients´ well-being and clinical outcome (median follow-up 88, range 33-97 months).

Results

In the estrogen receptor (ER) positive subgroup (n=50), high proliferation (MAI≥ 10) occurred more often in the carbohydrate group (CH) than in the fasting group (p=0.038). Progesterone receptor (PR) was more frequently negative in the CH-group (p=0.014). CH-patients had a significant between group rise in insulin (+ 24.31 mIE/L, 95% CI, 15.34 mIE/L to 33.27 mIE/L), insulin c-peptide (+ 1.39 nM, 95% CI, 1.03 nM to 1.77 nM), but reduced IGFBP3 levels (– 0.26 nM; 95% CI, ­– 0.46 nM to – 0.051 nM). CH-Intervention ER-positive patients had poorer relapse free survival (73%) than the fasting group (100%) (p=0.012; HR= 9.3 (95%CI, 1.1 to 77.7)). In the ER-positive patients, only tumor size (p=0.021; HR=6.07, 95%CI=1.31 to 28.03) and CH-or-fasting grouping (p=0.040; HR=9.30, 95% CI=1.11 to 77.82) had independent prognostic value. The adverse clinical outcome of carbohydrate loading occurred only in T2 patients with Relapse Free Survival of 100% in the fasting group vs. 33% in the CH-group (p=0.015; HR= inf). The CH-group reported less pain on day 5 and 6 compared to the control group (p<0.001) but showed otherwise no factors related to well-being.

Limitation

Only applicable to ER-positive breast cancer patients with T2-tumors.

Conclusions

Preoperative carbohydrate load increases proliferation and PR-negativity in ER-positive patients and worsens clinical outcome in ER-positive T2-patients.

Keywords: breast cancer, carbohydrate load, proliferation, insulin, insulin c-peptide, IGF-1, IGFBP3, tumor size, relapse free survival, breast cancer specific survival