bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of E-Service Quality and E-Trust on E-Customer Loyalty in the Bank Syariah Indonesia (BSI) Mobile E-Banking Application with E-Customer Satisfaction as an Intervening Variable (Case study on BSI customers in Bandar Lampung)
Authors: Rizkya Yunita Anggraini , Putri Rosydalina 
Published: November 2024

This study aims to analyze and prove the effect of E-Service Quality and E-Trust on E-Customer Loyalty in the e-Banking application of Bank Syariah Indonesia (BSI) Mobile with E-Customer Satisfaction as an Intervening variable this study there are problems at the level of use of BSI Mobile continues to increase, fraud cases, and service quality problems electronic (e-service quality) in BSI Mobile services where there are obstacles from the bank that is less responsive and less responsive. This study uses a quantitative approach using primary data and data collection by distributing questionnaires. The sample determinant uses Lemeshow formula so that the sample required is 100 respondents and the type of nonprobability sampling. Alat dalam menganalisis data adalah Structural Equation Modeling-Partial Least Square (SEM-PLS) dengan alat bantu software SmartPLS versi 4.0.Hasil penelitian menunjukkan bahwa E-Service Quality tidak berpengaruh signifikat terhadap E- Customer Loyalty ,E-trust berpengaruh positif dan signifikat terhadap E-Customer Loyalty.E-service Quality berpengaruh positif terhadap E-Customer Satisfaction .E-trust berpengaruh positif dan signifikat terhadap E-Customer Satisfaction .E-Customer Satisfaction berpengaruh positif dan signifikat terhadap E-Custumer Loyalty. E-Customer Satisfaction mampu memediasi hubungan positif dan signifikan antara E-Service Quality terhadap E-Loyalty. E-Customer Satisfaction mampu memediasi hubungan positif dan signifikan antara E-Trust terhadap E-Loyalty
DESENVOLVIMENTO E VALIDAÇÃO DE UM APLICATIVO MÓVEL PARA O SEGUIMENTO AMBULATORIAL DE PACIENTES CORONARIANOS
Authors: DUTRA SOBRAL POLLYANNA , PINHEIRO RAMOS VÂNIA , GOMES FERREIRA DA CRUZ VIRGÍNIA , DE LIMA CORDEIRO WAGNER , INGRID DA SILVA JADIANE , BARBOSA SILVA LIRA JÉSSICA , SANTOS DE SOUZA JÚNIOR BELARMINO 
Published: November 2024

As doenças cardiovasculares configuram-se como a maior causa de morbimortalidade no Brasil e no mundo. A doença arterial coronariana é multifatorial, em relação aos fatores de risco modificáveis, a educação em saúde é apontada como uma das políticas públicas mais eficazes para redução desses fatores e no controle da doença crônica. A introdução dos aplicativos móveis destaca-se como estratégia potencialmente efetiva na transmissão de conteúdo educativo, mudança de comportamento em saúde e gerenciamento da doença coronariana. Objetivo: analisar o processo de desenvolvimento e validação de um aplicativo móvel para o seguimento ambulatorial de pacientes com doença arterial coronariana. Método: trata-se de um estudo metodológico, de produção tecnológica que utilizou o método do Design Instrucional Contextualizado. Foi realizado o desenvolvimento de um aplicativo móvel (Conect Cor) para o seguimento ambulatorial de pacientes coronarianos, contendo informações sobre alimentação saudável, mudança de estilo de vida, lembretes de consultas, medicamentos e cadastro de sintomas. O desenvolvimento do aplicativo foi realizado através de um profissional de designer por meio do programa Appgyver. O conteúdo do aplicativo foi selecionado baseado em manuais e diretrizes do Ministério da Saúde e Sociedade Brasileira de Cardiologia. Para a validação de conteúdo e aparência a amostra foi composta por 22 juízes, especialistas em cardiologia e educação em saúde. Foram utilizados dois instrumentos validados, o Instrumento de Validação de Conteúdo Educativo em Saúde (IVCES) e o Instrumento de Validação de Tecnologias Educacionais em Saúde (IVATES). Resultados: os juízes consideraram o conteúdo do aplicativo válido e relevante com o IVC global de 0,92. O domínio que apresentou maior pontuação foi o de relevância, com IVC de 0,95. Todos os itens do instrumento de validação de aparência apresentaram valores superiores a 0,78, obtendo um IVA - T de 0,98 pontos, indicando que o aplicativo tem validade de aparência. Conclusão: O aplicativo móvel desenvolvido possui validade quanto ao conteúdo e aparência para o seguimento ambulatorial de pacientes com doença arterial coronariana, tornando-se não só uma tecnologia educacional, mas também uma ferramenta de auxílio para o paciente no gerenciamento da doença, mudança de estilo de vida, adesão ao regime terapêutico, prevenção secundária e promoção à saúde.
НАУКОВО - ТЕОРЕТИЧНІ ЗАСАДИ СТАНОВЛЕННЯ ПІДПРИЄМНИЦТВА У СІЛЬСЬКОМУ ЗЕЛЕНОМУ ТУРИЗМІ
Authors: ЖУРБА Ігор , ЦВІГУН Юрій 
Published: November 2024

У статті проаналізовано тенденції розвитку сільського зеленого туризму, які формуються на засадах наукових концепцій, теорій туризму, просторової економіки та економіко - географічних основ природокористування. Встановлено, що необхідно обґрунтувати найоптимальніші підходи та організаційно-економічні інструменти стимулювання суб’єктів сільського зеленого туризму, максимально якісно, без нанесення збитків для навколишнього середовища, розвивати сільський зелений туризм на мікро- та макро рівнях. Авторами доведено, що розробка наукових теорій та концепцій становлення туризму бере початок із кінця XIX початку та середини XX ст., це було викликано різким зростанням потоків відпочиваючих. Було з’ясувано суть теорії циклів еволюції туристичного простору враховуючи сьогоднішні умови становлення підприємництва у сфері сільського зеленого туризму. У 80-х роках XX ст. Р. Батлер розробив дану теорію, яка повязана із становленням туризму не тільки в окремій місцевості, а також в межах усього регіону.У статті обґрунтовано, що частина теорій та концепцій, які визначають особливості сучасного стану сільського зеленого туризму є поліфункіональні. Доведено, що вони враховують не тільки якість надання послуг, а також місце та роль туриста у нанесенні збитків навколишньому середовищу.
Analisis Minat Generasi Milenial Di Kelurahan Padang Rambun Untuk Melakukan Aktivitas Olahraga Di Ruang Terbuka Kelurahan Padang Rambun Kabupaten Seluma
Authors: Karlina Ria , Pujianto Dian , Raibowo Septian , Prabowo Andika , Permadi Andes 
Published: November 2024

Penelitian ini bertujuan untuk mengetahui hasil analisa minat generasi milenial untuk melakukan aktivitas olahraga. Penelitian ini menggunakan jenis penelitian deskriptif menggunakan pendekatan kualitatif. Penelitian ini menggunakan desain penelitian deskriptif analitis. Subjek penelitian ini adalah generasi milenial di Kelurahan Padang Rambun, objek penelitian ini adalah minat generasi milenial untuk melakukan aktivitas olahraga di Kelurahan Padang Rambun. Instrument penelitian yang digunakan berupa observasi, wawancara, angket dan dokumentasi. Berdasarkan hasil validasi ahli materi diperoleh hasil 87.2% dan 98.4% dikategorikan sangat layak untuk dibagikan ke responden. Berdasarkan hasil perhitungan deskriptif persentase diperoleh hasil dari faktor intrinsik minat generasi milenial diperoleh hasil rata-rata 70.9% kategori tinggi. Dari faktor ekstrinsik minat generasi milenial diperoleh rata-rata 74.5% kategori tinggi. Dari aktivitas olahraga minat generasi milenial diperoleh rata-rata 75.1% kategori tinggi. Dari hasil keseluruhan keseluruhan minat generasi milenial rata-rata 72.2% kategori tinggi.
Abstract
This study aims to determine the results of analyzing the millennial generation's interest in doing sports activities. This research uses descriptive research using a qualitative approach. This study used an analytical descriptive research design. The subject of this research is the millennial generation in Padang Rambun Village, the object of this research is the interest of the millennial generation in doing sports activities in Padang Rambun Ward. The research instruments used are observation, interviews, questionnaires and documentation. Based on the results of validation expert a result of 87.2% and a result of 98.4% categorized as very feasible to be distributed to respondents. Based on the results of the descriptive percentage calculation, the results of the intrinsic factors of millennial generation interest obtained an average result of 70.9% in the high category. From extrinsic factors, the interest of the millennial generation obtained an average of 74.5% in the high category. From sports activities, the interest of the millennial generation obtained an average of 75.1% in the high category. From the overall results, the overall interest of the millennial generation averaged 72.2% in the high category. It can be concluded that the overall interest of the millennial generation is categorized as high.
Regulation of α-Ketoglutarate levels by Myc affects metabolism and demethylation in porcine early embryos
Authors: Ding Ran , Zhou Yongfeng , Zhang Qi , Kong Xiangjie , Li Qi , Zhang Sheng , Chen Yibing , An Xinglan , Li Ziyi 
Published: November 2024

The Myc family is essential for cell proliferation, differentiation, and metabolism, particularly in embryonic development and stem cell functions. However, the specific role of Myc in porcine early embryonic development is not fully understood. This study observed high Myc expression during the four-cell stage of porcine embryos. Inhibition of Myc using 10058-F4 impaired embryonic development, disrupted energy metabolism, and increased DNA methylation. Mechanistically, these effects were dependent on α-KG, a TCA cycle intermediate and cofactor for TET demethylation enzymes. Sequencing analysis of four-cell embryos post-Myc inhibition revealed downregulation of key metabolic enzymes related to α-KG, such as CS, IDH2, leading to reduced α-KG levels. Supplementation with α-Ketoglutarate (α-KG) mitigated the negative effects of Myc inhibition, including lower blastocyst rates, decreased ATP levels, and increased 5 mC levels. In conclusion, Myc regulates the expression of key metabolic enzymes during the four-cell stage, influencing early embryonic metabolism and epigenetic reprogramming.
Case report: The outcomes of neoadjuvant immunotherapy combined with chemotherapy in pulmonary sarcomatoid carcinoma: case series and literature review
Authors: Guo Xiaokang , Wang Jingjing , Li Daosheng , Wang Bin , Zhu Hui , Guo Hongbo 
Published: November 2024

BackgroundPulmonary sarcomatoid carcinoma (PSC) is a highly aggressive malignancy with a significant risk of recurrence even after surgical intervention, leading to a dismal prognosis. In recent years, perioperative immunotherapy has demonstrated promising results in resectable non-small cell lung cancer (NSCLC). However, there is a lack of studies reporting the efficacy of perioperative immunotherapy in PSC.Case presentationWe report the clinical outcomes of four patients diagnosed with locally advanced PSC who underwent neoadjuvant immunotherapy in combination with chemotherapy from 2021 to 2023 in our hospital. Prior to surgery, these patients received 2 to 4 cycles of neoadjuvant treatment. Post-treatment imaging assessments indicated a partial response (PR) in all cases, and each patient successfully achieved R0 resection. Pathological evaluations demonstrated significant pathological responses: one patient attained Pathological Complete Response (PCR), two patients exhibited Major Pathological Response (MPR), and one patient showed PR. Currently, all four patients remain alive without evidence of tumor progression. Notably, the patient who achieved PCR has maintained a disease-free survival (DFS) exceeding 32 months post-surgery, while their event-free survival (EFS) has surpassed 36 months.ConclusionsNeoadjuvant immunotherapy in combination with chemotherapy has provided new promise for the treatment of locally advanced PSC with surgical potential. But these findings still need to be verified by further prospective researches.
Effects of COVID-19 virus-like particles on the behavioral and cognitive performance of human apolipoprotein E targeted replacement mice
Authors: O’Niel Abigail , Pederson Alexandra , Saltontall Elizabeth , Nguyen Kayla , Pantoja Monzerrat , Chaudhari Mitali , Sandholm Phoebe , Yoon Eric , Harrison Henry F. , Boutros Sydney , Hirsch Alec J. , Raber Jacob 
Published: November 2024

IntroductionThe effects of viral infections might be apolipoprotein E (apoE) isoform-dependent. In humans, there are three major apoE isoforms, E2, E3, and E4. E4 is associated with the enhanced entry of several viruses into the brain and their disease progression. A concern of infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the development of post-acute COVID-19 syndrome, also known as long COVID. Genetic risk factors for developing long COVID were reported.MethodsIn this study, we used virus-like particles (VLPs) that include expression of the SARS-CoV-2 nucleocapsid (N), membrane (M), and envelope (E) structural proteins together with S. In the current study, we used human E2, E3, and E4 targeted replacement mice to assess whether these VLPs affect body weight, behavioral and cognitive performance, and circadian body temperatures. Using VLPs allow working outside an ABSL-3 facility.ResultsThe effects of VLPs on some behavioral measures were apoE isoform-dependent, with the E2 mice being more affected than E3 or E4 mice. The overall decreased activity in the open field containing objects in week 2 indicate that VLPs can also reduce activity levels in an apoE isoform-independent fashion.DiscussionThe results of the current study indicate that even in the absence of viral replication, detrimental effects of VLPs on behavioral measures and circadian body temperatures are seen.
Species identification of modern and archaeological shark and ray skeletal tissues using collagen peptide mass fingerprinting
Authors: Buckley Michael , Oldfield Ellie-May , Oliveira Cristina , Boulanger Clara , Kitchener Andrew C. , Fuller Nicole R. , Ardren Traci , Thompson Victor D. , Fitzpatrick Scott M. , LeFebvre Michelle J. 
Published: November 2024

IntroductionElasmobranchs, such as sharks and rays, are among the world’s most endangered vertebrates, with over 70% loss in abundance over the past 50 years due to human impacts. Zooarchaeological baselines of elasmobranch diversity, distribution, and exploitation hold great promise for contributing essential historical contexts in the assessment of contemporary patterns in their taxonomic diversity and vulnerability to human-caused extinction. Yet, the historical ecology of elasmobranchs receives relatively less archaeological attention compared to that of ray-finned fishes or marine mammals, largely due to issues of taxonomic resolution across zooarchaeological identifications.MethodsWe explore the use of Zooarchaeology by Mass Spectrometry (ZooMS) for species identification in this unstudied group, using an archaeological case study from the marine environments of the Florida Keys, a marine biodiversity hotspot that is home to an array of elasmobranch species and conservation efforts. By comparison with 39 modern reference species, we could distinguish 12 taxa within the zooarchaeological assemblage from the Clupper archaeological site (Upper Matecumbe Key) that included nine sharks, two rays and a sawfish.Results and discussionThe results indicate that, through additional complexity of the collagen peptide mass fingerprint, obtained due to the presence of the cartilaginous type II collagen, ZooMS collagen peptide mass fingerprinting provides exceptionally high taxonomic resolution in this group, yielding species-level identifications in all cases where sufficient reference material was used. This case study also highlights the added value of ZooMS for taxa that are more difficult to distinguish in zooarchaeological analyses, such as vertebrae of the Atlantic sharpnose shark (Rhizoprionodon terraenovae) and the hammerhead sharks (Sphyrna spp.) in the Florida Keys. Therefore, the application of collagen peptide mass fingerprinting to elasmobranchs offers great potential to improve our understanding of their archaeological past and historical ecology.
Effectiveness of sofosbuvir-based treatments for patients with hepatitis C virus genotype 6 infection: a real-world study from East China
Authors: Jiang Fan-Rong , Ye Xiao-Ting , Huang He-Qing , Hu Yu-Tao , Wang Dong-Hui , Jiang Su-Wen , Wang Jia-Lan , Hu Ai-Rong 
Published: November 2024

BackgroundOver the past decade, the proportion of hepatitis C virus (HCV) genotypes (GT) 1 and 2 has decreased in almost all regions of China, while GT 3 and 6 have emerged as new challenges. GT 6 is unique in many respects, like high genetic variability and emerging resistant variants. This study aims to assess the efficacy of sofosbuvir (SOF)-based treatments in patients with GT 6 chronic hepatitis C (CHC).MethodsA retrospective analysis was conducted on patients with GT 6 HCV infection, who were diagnosed between July 2018 and May 2023. All patients received a 12-week course of SOF-based treatments. The primary efficacy endpoint was sustained virologic response (SVR), which is defined as having undetectable HCV RNA at 12 weeks after treatment completion (SVR12). The efficacy data for SVR12 were analyzed using both the evaluated population (EP) and per-protocol population (PP). For the PP populations, efficacy data were stratified using Forrester plots.ResultsA total of 201 patients were included in the study. In PP population, the end of treatment virological response rate was 99.48% (190/191), the SVR12 rate was 99.31% (143/144), and the SVR24 rate was 100.00% (75/75). Only one patient with genotype 6a experienced a relapse 12 weeks after the completion of treatment, but her HCV RNA was undetectable both at the end of treatment and 24 weeks after the end of treatment. Additionally, the normalization rates of alanine transaminase (ALT) and aspartate aminotransferase (AST) were significantly higher at the end of treatment (EOT) compared to baseline (27.36% vs. 93.03%, 36.32% vs. 95.02%, p &lt; 0.001). Significant improvements were observed in the levels of total bilirubin, ALT, AST, albumin, globulin, albumin/globulin ratio, gamma-glutamyl transferase, alkaline phosphatase, platelet, fibrosis-4 (FIB-4), and aspartate transaminase to platelet ratio index (APRI) between baseline and EOT (p &lt; 0.05).ConclusionSOF-based treatments achieved high virological and biochemical response rates in patients with HCV GT 6 infection.
Gut microbiota profiling in injection drug users with and without HIV-1 infection in Puerto Rico
Authors: Aluthge Nirosh , Adams Seidu , Davila Carmen A. , Gocchi Carrasco Nova R. , Chiou Kathy S. , Abadie Roberto , Bennett Sydney J. , Dombrowski Kirk , Major Angel M. , Valentín-Acevedo Aníbal , West John T. , Wood Charles , Fernando Samodha C. 
Published: November 2024

IntroductionThe full extent of interactions between human immunodeficiency virus (HIV) infection, injection drug use, and the human microbiome is unclear. In this study, we examined the microbiomes of HIV-positive and HIV-negative individuals, both drug-injecting and non-injecting, to identify bacterial community changes in response to HIV and drug use. We utilized a well-established cohort of people who inject drugs in Puerto Rico, a region with historically high levels of injection drug use and an HIV incidence rate disproportionately associated with drug use.MethodsUsing amplicon-based 16S rDNA sequencing, we identified amplicon sequence variants (ASVs) that demonstrated significant variations in the composition of microbial communities based on HIV status and drug use.Results and discussionOur findings indicate that the HIV-positive group exhibited a higher abundance of ASVs belonging to the genera Prevotella, Alloprevotella, Sutterella, Megasphaera, Fusobacterium, and Mitsuokella. However, Bifidobacteria and Lactobacillus ASVs were more abundant in injectors than in non-injectors. We examined the effect of drug use on the gut microbiome in both HIV-infected and non-infected patients, and found that multiple drug use significantly affected the microbial community composition. Analysis of differential of bacterial taxa revealed an enrichment of Bifidobacterium spp., Faecalibacterium spp., and Lactobacillus spp. in the multiple drug-injecting group. However, in the non-injecting group, Parabacteroides spp., Prevotella spp., Paraprevotella spp., Sutterella spp., and Lachnoclostridium spp. The presence of multiple drug-injecting groups was observed to be more prevalent. Our findings provide detailed insight into ASV-level changes in the microbiome in response to HIV and drug use, suggesting that the effect of HIV status and drug injection may have different effects on microbiome composition and in modulating gut bacterial populations.
Comprehensive prognostic gene identification and functional characterization of GRAMD1A in Wilms tumor: development of risk prediction models and therapeutic implications
Authors: Zeng Qiang , Tao Junfeng , Qin Lilu , Zeng Yong , Liu Zhong , Xu Mingxian , Zeng Linshan 
Published: November 2024

BackgroundWilms tumor (WT) is the most common pediatric kidney cancer, with survival rates exceeding 90% in localized cases. However, advanced or recurrent WT remains difficult to treat due to poor prognosis and limited knowledge of its molecular mechanisms. Gene expression profiling has shown promise in identifying prognostic markers and therapeutic targets. This study aimed to identify key prognostic genes and pathways in WT, construct risk prediction models, and validate their role in tumor progression.MethodsRNA sequencing and clinical data from 136 WT patients were obtained from the TARGET database. Differential gene expression analysis was conducted using GEO datasets GSE11024 and GSE66405 to compare WT and normal kidney tissues. Identified differentially expressed genes (DEGs) underwent Gene Ontology (GO) and KEGG pathway enrichment analysis to explore biological functions and pathways associated with WT progression. Univariate Cox regression was used to assess the association between DEGs and overall survival (OS) and progression-free survival (PFS). LASSO regression models were developed for risk stratification, and model accuracy was evaluated using time-dependent ROC curves. External validation confirmed key hub genes, while functional assays in WT cell lines (WiT-49) assessed the role of GRAMD1A in tumor behavior.ResultsA total of 3,395 DEGs were identified, with 1,564 upregulated and 1,831 downregulated genes. Enrichment analyses revealed significant pathways involved in cell cycle regulation and metabolic reprogramming. Six key genes (GRAMD1A, PLXNA3, SPR, EBAG9, RBM47, and RIDA) were associated with both OS and PFS. LASSO models demonstrated strong predictive performance, with GRAMD1A identified as a major risk factor. External validation confirmed differential expression, and functional assays showed that GRAMD1A silencing significantly inhibited WT cell viability, proliferation, migration, and invasion.ConclusionsThis study identifies novel prognostic genes and potential therapeutic targets in WT. GRAMD1A, SPR, EBAG9, RBM47, and RIDA play critical roles in WT progression, with GRAMD1A as a key oncogenic factor, offering potential for risk stratification and future therapeutic intervention.