bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does sharp force trauma alter blow fly attraction to, colonization of, and decomposition of vertebrate remains?
Authors: Munro Holly L. , Mondor Edward B. , Lampert Evan C. 
Published: March 2019

AbstractForensic entomologists use insect evidence to estimate the minimum postmortem interval of human remains. However, how different forms of trauma influence insect attraction to, and colonization of, the body is poorly understood. Our objective was to identify the Calliphoridae community present in the Piedmont region (Georgia, USA), and determine whether postmortem sharp force trauma (none vs. slashed vs. stabbed) altered insect attraction, colonization, and the resulting decomposition rate of stillborn pigs (Suidae). We show that the limited blow fly community is dominated by Lucilia coeruleiviridis Macquart (Diptera: Calliphoridae). Third instars (L3) of L. coeruleiviridis were found on control pigs at 48 h, slashed pigs at 36 h, and stabbed pigs at 24 h. Decomposition rate, as determined by total body score, was greater in the first 12 h in stabbed pigs, compared to control and slashed pigs. Further experimentation is required to determine the effect of different forms of trauma on insect attraction, colonization, and resulting vertebrate decomposition rates.
Mycobacterium tuberculosis UvrD1 and UvrD2 helicases unwind G‐quadruplex DNA
Authors: Saha Tias , Shukla Kaustubh , Thakur Roshan Singh , Desingu Ambika , Nagaraju Ganesh 
Published: March 2019

Unresolved G‐quadruplex (G4) DNA secondary structures impede DNA replication and can lead to DNA breaks and to genome instability. Helicases are known to unwind G4 structures and thereby facilitate genome duplication. Escherichia coli UvrD is a multifunctional helicase that participates in DNA repair, recombination and replication. Previously, we had demonstrated a novel role of E. coli UvrD helicase in resolving G4 structures. Mycobacterium tuberculosis genome encodes two orthologs of E. coli UvrD helicase, UvrD1 and UvrD2. It is unclear whether UvrD1 or UvrD2 or both helicases unwind G4 DNA structures. Here, we demonstrate that M. tuberculosis UvrD1 and UvrD2 unwind G4 tetraplexes. Both helicases were proficient in resolving previously characterized tetramolecular G4 structures in an ATP hydrolysis and single‐stranded 3′‐tail‐dependent manner. Notably, M. tuberculosis UvrD1 and UvrD2 were efficient in unwinding G4 structures derived from the potential G4 forming sequences present in the M. tuberculosis genome. These data suggest an extended role for M. tuberculosis UvrD1 and UvrD2 helicases in resolving G4 DNA structures and provide insights into the maintenance of genome integrity via G4 DNA resolution.
Age‐associated alterations in murine dermis through inflammatory response with mitochondrial DNA deletions
Authors: Kawaguchi Koichiro , Kim Sang‐Eun , Sugiyama Daijiro , Sugimoto Masataka , Maruyama Mitsuo 
Published: March 2019

AimSkin aging is caused by intrinsic and extrinsic mechanisms. Because it is difficult to distinguish intrinsic mechanisms from extrinsic skin aging, the mechanisms of intrinsic skin aging remain unclear. The present study aimed to characterize age‐associated alterations in murine skin and investigate the mechanisms of intrinsic skin aging.MethodsWe measured morphological changes in dorsal skin from young (aged 2 months) and old (aged 22–24 months) mice by histological analysis. Age‐associated alteration of gene expression patterns was determined by quantitative polymerase chain reaction and immunohistochemistry. Reactive oxygen species production in mouse dorsal skin was detected by confocal laser scanning microscopy. Mitochondrial DNA deletions were detected by conventional polymerase chain reaction and quantitative polymerase chain reaction analyses.ResultsChronologically aged skin had dermal atrophy caused by increased matrix‐degrading enzymes and decreased collagen synthesis. Chronologically aged skin samples also had increased senescence‐associated secretory phenotype factors, elevated reactive oxygen species production and a higher frequency of the mitochondrial DNA common deletion.ConclusionsThese observations suggest that chronological skin aging is associated with increased frequency of the mitochondrial DNA common deletion and chronic inflammation through the reactive oxygen species–senescence‐associated secretory phenotype axis. Geriatr Gerontol Int 2019; 19: 451–457.
Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis
Authors: Bilir Şükriye , Kojidani Tomoko , Mori Chie , Osakada Hiroko , Kobayashi Shouhei , Koujin Takako , Hiraoka Yasushi , Haraguchi Tokuko 
Published: March 2019

AbstractReassembly of the nuclear pore complex (NPC) at the end of mitosis is an important event for eukaryotic nuclear function. In this study, we examined the dynamic behaviors of the endoplasmic reticulum (ER) by “Live CLEM” imaging. In metaphase, numerous fenestrations on the ER membrane were observed around chromosomes. In telophase, these fenestrations became filled at the region attached to chromosomes, whereas they remained open at the region unattached to chromosomes, suggesting that NPC assembly takes place at fenestrations on the membrane. To determine the roles of nucleoporins in postmitotic NPC formation, we used artificial beads conjugated with anti‐GFP antibody, which captures GFP‐fused proteins on the beads when incorporated into cells. Live CLEM imaging of telophase cells containing Nup133‐coated beads or Nup153‐coated beads showed that Nup133 and Nup153, as the sole effector molecules, assembled the NPC‐like structure on the membrane fenestrations. Indirect immunofluorescence staining of the Nup133‐coated beads showed that Nup133 effectively assembled Nup107 and ELYS, whereas minimal assembly of Nup98 and Nup62 was observed; the Nup153‐coated bead effectively assembled Nup98, Nup62 and Pom121, but assembled neither Nup107 nor ELYS. Our results suggest that Nup133 and Nup153 play different roles in assembling the NPC on membrane fenestrations.
Histological ageing of fractures in infants: a practical algorithm for assessing infants suspected of accidental or non‐accidental injury
Authors: Naqvi Anie , Raynor Emma , Freemont Anthony J 
Published: March 2019

AimsThis study is the first to systematically document histological features of fractures of known age in infants (≦12 months). It has been used to develop a tabulated database specifically to guide histopathologists to age fractures in children considered to have suffered accidental or non‐accidental injury (NAI). Currently in the United Kingdom there are insufficient pathologists with experience in histological ageing of fractures to meet the medicolegal need for this examination. This study provides a practical tool that will allow those skilled paediatric and forensic pathologists currently involved in assessing infants for evidence of accidental or non‐accidental injury a basis for extending their assessment into this area of unmet need.Methods and resultsOne hundred and sixty‐nine fractures of known age at death were obtained from 52 anonymised infants over a period of 32 years (1985–2016 inclusive). Sections stained using haematoxylin and eosin (H&E) and Martius scarlet blue (MSB) were used to identify specific histological features and to relate them to fracture age. In 1999 the data were entered into a tabulated database for fractures accumulated between from 1985 to 1998 inclusive. Thereafter cases were added, and at 2‐yearly intervals the accumulated data were audited against the previous database and adjustments made.ConclusionsThis paper describes the final data set from the 2017 audit. The study was terminated at the end of 2016, as there had been no material changes in the data set for three consecutive audits.
Micro‐ribonucleic acid expression signature of metastatic castration‐resistant prostate cancer: Regulation of NCAPH by antitumor miR‐199a/b‐3p
Authors: Arai Takayuki , Kojima Satoko , Yamada Yasutaka , Sugawara Sho , Kato Mayuko , Yamazaki Kazuto , Naya Yukio , Ichikawa Tomohiko , Seki Naohiko 
Published: March 2019

ObjectivesTo identify oncogenes regulated by micro‐ribonucleic acid, miR‐199a/b‐3p, in metastatic castration‐resistant prostate cancer.MethodsAdvanced ribonucleic acid sequencing technologies were applied to construct a micro‐ribonucleic acid expression signature using metastatic castration‐resistant prostate cancer autopsy specimens. Ectopic expression of mature micro‐ribonucleic acids or small‐interfering ribonucleic acids were applied to functional assays for cancer cell lines. Genome‐wide gene expression and in silico database analyses were carried out to predict micro‐ribonucleic acid targets.ResultsEctopic expression of miR‐199a/b inhibited cancer cell aggressiveness. The gene coding for non‐structural maintenance of chromosomes condensin I complex subunit H was directly regulated by miR‐199a/b‐3p. High expression of condensin I complex subunit H was significantly associated with poor disease‐free survival by The Cancer Genome Atlas database analysis (P < 0.0001). Overexpression of condensin I complex subunit H was detected in hormone‐sensitive prostate cancer and castration‐resistant prostate cancer specimens, and knockdown assays showed that its expression enhanced cancer cell migration and invasive abilities.ConclusionsSmall ribonucleic acid sequencing of metastatic castration‐resistant prostate cancer specimens showed the presence of several antitumor micro‐ribonucleic acids whose targets are involved in hormone‐sensitive prostate cancer and metastatic castration‐resistant prostate cancer pathogenesis. Condensin I complex subunit H seems to be a promising diagnostic marker and therapeutic target for this disease. Our approach, based on the roles of anti‐tumor micro‐ribonucleic acids and their targets, will contribute to an improved understanding of the molecular pathogenesis of hormone‐sensitive prostate cancer and metastatic castration‐resistant prostate cancer.
Feel good now or regret it later? The respective roles of affective attitudes and anticipated affective reactions for explaining health‐promoting and health risk behavioral intentions
Authors: Stevens Courtney J. , Gillman Arielle S. , Gardiner Casey K. , Montanaro Erika A. , Bryan Angela D. , Conner Mark 
Published: March 2019

AbstractEvidence supporting the incorporation of affective constructs, such as affective attitudes and anticipated regret, into theoretical models of health behavior has been mounting in recent years; however, the role of positive anticipated affective reactions (e.g., pride) has been largely unexplored. The purpose of the present investigation was to assess how affective attitudes and anticipated affective reactions (both pride and regret for performing a behavior or not) may provide distinct utility for understanding intentions to perform health‐promoting and health risk behaviors over and above cognitive attitudes and other established theoretical constructs from the theory of planned behavior (TPB). Participants (N = 210) were recruited via Amazon’s Mechanical Turk to complete a one‐time online battery assessing TPB and affective constructs. Self‐reported intentions served as the main outcome measure, and hierarchical linear modeling was used to examine the effects of TPB and affective constructs across behaviors. Controlling for TPB constructs, more positive affective attitudes and greater anticipated regret, but not anticipated pride, predicted intentions to engage in future health behaviors. Anticipated affective reactions contributed explanatory variance for intentions to perform health risk behaviors, but anticipated pride and regret were not associated with intentions to perform health risk behaviors. Contributions made via the inclusion of both positively and negatively valence anticipated affective reactions for both action and inaction (performing a behavior or not) across a range of health promoting and health risk behaviors are discussed, as well as implications for future intervention work.
Defining Supply Chain Management: In the Past, Present, and Future
Authors: Min Soonhong , Zacharia Zach G. , Smith Carlo D. 
Published: March 2019

The article titled “Defining Supply Chain Management” published in 2001 in the Journal of Business Logistics has been cited over 4,900 times in the last 17 years. In this paper, we first provide a historical review of how the article originated and the contributions the article made to both the theory and practice of supply chain management (SCM). Next, we highlight the key market and technological changes that have emerged in SCM followed by how the theory proposed in the 2001 article can still be relevant to support SCM research and practice going forward. We also propose ways of configuring a supply chain and partnering across companies to serve customers in an optimal way. We conclude with a call for research on developing new frameworks to better describe, explain, predict, and shed light on the evolving nature of SCM.
A functional interaction between Hippo‐YAP signalling and SREBPs mediates hepatic steatosis in diabetic mice
Authors: Shu Zhiping , Gao Yuan , Zhang Guopeng , Zhou Yu , Cao Jing , Wan Dongyi , Zhu Xiaohua , Xiong Wenqian 
Published: March 2019

AbstractThe Hippo pathway is an evolutionarily conserved regulator of organ size and tumorigenesis that negatively regulates cell growth and survival. Whether the Hippo pathway regulates cell metabolism is unknown. Here, we report that in the nucleus of hepatocytes, Yes‐associated protein(YAP)—the terminal effector of the Hippo pathway—directly interacts with sterol regulatory element binding proteins (SREBP‐1c and SREBP‐2) on the promoters of the fatty acid synthase (FAS) and 30‐hydroxylmethyl glutaryl coenzyme A reductase (HMGCR), thereby stimulating their transcription and promoting hepatocyte lipogenesis and cholesterol synthesis. In diet‐induced diabetic mice, either Lats1 overexpression or YAP knockdown protects against hepatic steatosis and hyperlipidaemia through suppression of the interaction between YAP and SREBP‐1c/SREBP‐2. These results suggest that YAP is a nuclear co‐factor of SREBPs and that the Hippo pathway negatively affects hepatocyte lipogenesis by inhibiting the function of YAP‐SREBP complexes.
Fracture analysis of monolithic CAD‐CAM crowns
Authors: Kashkari Afnan , Yilmaz Burak , Brantley William A. , Schricker Scott R. , Johnston William M. 
Published: March 2019

AbstractPurposeThe aim of this study was to compare the integrity of zirconia, lithium disilicate, and zirconia‐reinforced lithium silicate CAD‐CAM crowns after being subjected to cyclic loading and then subjected to static loading until fracture.Material and MethodsZirconia (Zirkonzahn), lithium disilicate (LDS, Ivoclar Vivadent AG), and zirconia‐reinforced lithium silicate glass ceramic (ZLS) (Vita Suprinity, Vita Zahnfabrik) monolithic crowns were milled (n = 6). The crowns were bonded using composite resin cements and subjected to cyclic loading under wet conditions. Three specimens from each group were loaded for 10 000 cycles, and the other three specimens were loaded for 50 000 cycles with 250 N. Specimens were subjected to loading until fracture. Load‐to‐fracture values were analyzed with 2‐way (ANOVA) and Tukey‐Kramer post hoc test (α = 0.05). Specimens from each group were examined using an SEM.ResultsMean load‐to‐fracture values among materials were significantly different from each other (P < 0.05). No significant effect of the number of cycles was found on the load‐to‐fracture values of crowns (P > 0.05).ConclusionLoad‐to‐fracture values of zirconia were higher than those of LDS, which were higher than those of ZLS. The number of fatigue loading cycles did not affect the load‐to‐fracture of the tested crowns for a given material.Clinical SignificanceMore research needs to be conducted before considering the routine use of ZLS for molars in patients with high risk of parafunctional habits.
Human amnion epithelial cells and their soluble factors reduce liver fibrosis in murine non‐alcoholic steatohepatitis
Authors: Kuk Nathan , Hodge Alexander , Sun Ying , Correia Jeanne , Alhomrani Majid , Samuel Chrishan , Moore Gregory , Lim Rebecca , Sievert William 
Published: March 2019

AbstractBackground and AimNon‐alcoholic steatohepatitis (NASH) can lead to cirrhosis and hepatocellular carcinoma. Currently, lifestyle modification is the only effective treatment. We have shown that human amnion epithelial cells (hAECs) reduce inflammation and fibrosis in toxin‐induced liver injury models. We examined the effect of these cells and the soluble factors released by the cells into culture medium (hAEC conditioned medium [hAEC‐CM]) in a diet‐induced murine NASH model.MethodsC57BL/6J male mice received a Western “fast food diet” for 42 weeks. Group 1 received an intraperitoneal injection of 2 × 106 hAECs at week 34, group 2 received an additional hAEC dose at week 38, and group 3 received thrice weekly hAEC‐CM injections intraperitoneal for 8 weeks from week 34. Liver fibrosis area, inflammation, and fibrosis regulators were measured by immunohistochemistry, qPCR, and gelatin zymography. Metabolic parameters were also assessed.ResultsFast food diet‐fed mice demonstrated peri‐cellular hepatic fibrosis, inflammation, and steatosis typical of NASH. Liver fibrosis area was reduced by 40% in hAEC‐treated and hAEC‐CM‐treated mice. hAEC treatment significantly reduced pSMAD 2/3 signaling and the number of activated hepatic stellate cells and liver macrophages. Matrix metalloproteinase 2 and 9 gene and protein expression were variably affected. hAEC treatment did not alter the NASH activity score or metabolic parameters such as bodyweight, total cholesterol, or glucose tolerance.ConclusionHuman amnion epithelial cell and hAEC‐CM significantly reduced hepatic inflammation and fibrosis in a diet‐induced non‐alcoholic fatty liver disease model. Although hAEC and hAEC‐CM did not affect the metabolic components of NASH, their therapeutic potential is promising and warrants further investigation.
Identification of Binge Eating Disorder Criteria: Results of a National Survey of Healthcare Providers
Authors: Chao Ariana M. , Rajagopalan Adithi V. , Tronieri Jena Shaw , Walsh Olivia , Wadden Thomas A. 
Published: March 2019

AbstractPurposeTo determine whether general healthcare providers and adult psychiatrists recognized binge eating disorder (BED) symptoms and features. The aims were to examine how they delineated the core criteria ofBED—eating a large amount of food and sense of loss of control over eating—and how their evaluations compared to ratings byBEDexperts.DesignThis is a cross‐sectional study of a nationwide U.S. sample of healthcare providers and a convenience sample ofBEDexperts.MethodsProviders were mailed surveys that asked respondents about their perceptions of a large amount of food and whether they thought case vignettes met thresholds for loss of control. Participants were also asked to selectBEDdiagnostic criteria from a symptom list. Results were analyzed using one‐way analyses of variance with post‐hoc comparisons and chi‐squared tests.FindingsThe survey was completed by 405 healthcare providers (response rate of 28.4%). Ratings of a large amount of food did not differ betweenBEDexperts and general healthcare providers (p= .10) or psychiatrists (p = .90). Provider groups did not differ significantly on whether five of the six vignettes met thresholds for loss of control (p> .05). Of the respondents, 93.0% of general healthcare providers and 88.6% of psychiatrists could not correctly identify the diagnostic criteria forBED.ConclusionsAcross provider groups, demarcation of a large amount of food and loss of control over eating were relatively consistent. However, general healthcare providers and psychiatrists were not able to correctly identifyBEDsymptoms.Clinical RelevanceTraining and education are greatly needed to improve knowledge of the diagnostic criteria forBED.
Economic Resources, Cultural Matching, and the Rural–Urban Boundary in China's Marriage Market
Authors: Zhou Yun 
Published: March 2019

Objective: This article investigates marital sorting by household registration status (hukou) and education in contemporary urban China, paying special attention to individuals who have achieved rural‐to‐urban hukou mobility before marriage.Background: Existing theoretical frameworks of assortative mating have highlighted economic resources and cultural matching as two key dimensions. These two frameworks place a different emphasis on individuals' achieved versus ascriptive characteristics and hold different implications for understanding the link between marital sorting patterns and social openness or closure. With a focus on hukou converters, this study examines the relative importance of achieved versus ascriptive traits in China's marriage market and contributes to the evaluation of the two frameworks.Method: This study adopts a mixed‐methods approach. The quantitative analysis uses the harmonic mean marriage function to analyze the nationally representative 2006 China General Social Survey. The qualitative data consist of 115 in‐depth interviews collected in two Chinese metropolitan areas between 2016 and 2017.Results: Quantitative results showed that hukou converters and urban‐born individuals had the highest propensity of marrying a spouse of the same hukou trajectory. Qualitative results further revealed the gap between converters and their urban‐born peers to be hierarchical. Although both groups emphasized the importance of shared values and habitus in evaluating prospective partners, urban‐born individuals regarded hukou converters as a culturally distinct and less desirable option.Conclusion: The findings highlight the power of cultural matching in China's urban marriage market. Hukou converters' rural origin remains visible and acts as a source of lasting symbolic distinction despite adulthood status attainment.
In and Out of Balance: Industry Relatedness, Learning Capabilities and Post‐Acquisition Innovative Performance
Authors: Cefis Elena , Marsili Orietta , Rigamonti Damiana 
Published: March 2019

AbstractThe existence of an inverted U‐shaped effect of the relatedness between acquirer and acquired firm on the innovative performance subsequent to an acquisition is normally regarded as indicative of the existence of a trade‐off between exploration and exploitation in external innovation search. We argue that acquirers endowed with heterogeneous learning capabilities can alter the shape of the trade‐off to their favour. In particular, we focus on a notion of industry relatedness that captures the coherence between the domains of operation of the acquirer and the acquired firm. Using a longitudinal dataset of 1,736 domestic acquisitions in the Netherlands, we show that the heterogeneous learning capabilities of the acquirers alter the shape of the inverted‐U relationship, according to first‐ and second‐order moderating effects. Our results confirm that learning capabilities by internal R&D and by acquisition experience both improve what acquirers can achieve in innovative performance when industry relatedness is at the point of balance between exploration and exploitation. In contrast, they have opposite implications on the potential losses in innovative performance when industry relatedness is outside the point of balance: internal R&D increases the tolerance of the trade‐off, smoothing out potential innovation losses, whereas acquisition experience reduces it.