bluuebunny/crossref_metadata_2025_split huggingface dataset:
Confounding by indication of the safety of de-escalation in community-acquired pneumonia: a simulation study embedded in a prospective cohort
Authors: van Heijl Inger , Schweitzer Valentijn A. , Boel C.H. Edwin , Oosterheert Jan Jelrik , Huijts Susanne M. , Dorigo-Zetsma Wendelien , van der Linden Paul D. , Bonten Marc J.M. , van Werkhoven Cornelis H. 
Published: May 2019

BackgroundObservational studies have demonstrated that de-escalation of antimicrobial therapy is independently associated with lower mortality. This most probably results from confounding by indication. Reaching clinical stability is associated with the decision to de-escalate and with survival. However, studies rarely adjust for this confounder. We quantified the potential confounding effect of clinical stability on the estimated impact of de-escalation on mortality in patients with community-acquired pneumonia.MethodsData were used from the Community-Acquired Pneumonia immunization Trial in Adults (CAPiTA). The primary outcome was 30-day mortality. We performed Cox proportional-hazards regression with de-escalation as time-dependent variable and adjusted for baseline characteristics using propensity scores. The potential impact of unmeasured confounding was quantified through simulating a variable representing clinical stability on day three, using data on prevalence and associations with mortality from the literature.ResultsOf 1,536 included patients, 257 (16.7%) were de-escalated, 123 (8.0%) were escalated and in 1156 (75.3%) the antibiotic spectrum remained unchanged. The adjusted hazard ratio of de-escalation for 30-day mortality (compared to patients with unchanged coverage), without adjustment for clinical stability, was 0.36 (95%CI: 0.18-0.73). If 90% to 100% of de-escalated patients were clinically stable on day three, the fully adjusted hazard ratio would be 0.53 (95%CI: 0.26-1.08) to 0.90 (95%CI: 0.42-1.91), respectively. The simulated confounder was substantially stronger than any of the baseline confounders in our dataset.ConclusionsWith plausible, literature-based assumptions, clinical stability is a very strong confounder for the effects of de-escalation. Quantification of effects of de-escalation on patient outcomes without proper adjustment for clinical stability results in strong negative bias. As a result, the safety of de-escalation remains to be determined.
The polymorphic PolyQ tail protein of the Mediator Complex, Med15, regulates variable response to stress
Authors: Gallagher Jennifer E.G. , Nassif Casey , Pupo Amaury 
Published: May 2019

AbstractThe Mediator is a multi-protein complex composed of subunits called head, body, tail, and CDK that is conserved from yeast to humans and plays a central role in transcription. However, not all the components are required for basal transcription. Components of the tail are not essential but to varying degrees are required for growth in different stresses. While some stresses are familiar such as heat, desiccation, and starvation, others are exotic, yet yeast can elicit a successful stress response. MCHM is a hydrotrope that induces growth arrest in yeast. By exploiting genetic variation, specifically in Med15, between yeast strains, we found that a naturally occurring Med15 allele with polyQ (polyglutamine) expansion conferred MCHM sensitivity. Expansion in polyQ repeat can induce protein aggregation and in humans can cause neurodegenerative diseases. In yeast, the MCHM sensitivity was not from a loss of function as the reciprocal hemizygous hybrids were all sensitive and the homozygous null mutant was less sensitive than the hemizygous hybrids. This suggests that there is an incompatibility between Mediator components from genetic divergent yeast strains. Transcriptomics from yeast expressing the incompatible Med15 (longer polyQ repeats in the strain with fewer repeats) changed gene expression in diverse pathways. Med15 protein existed in multiple isoforms, mostly from likely post-translational modifications and different alleles have different patterns of isoforms. Stability of both alleles of Med15 was dependent on Ydj1, a J-type chaperone. The protein level of the incompatible Med15 allele was lower than the compatible allele and was turned over faster. Med15 is tethered to the rest of the Mediator complex via Med2 and 3. Deletion of either Med2 or Med3 changed the Med15 isoform patterns in a similar manner. Whereas deletion of Med5, a distal component of the Mediator tail, did not change the pattern. Themed2andmed3mutants were similarly sensitive to MCHM whilemed5mutants were not. Differences in the phenotype of yeast carrying different Med15 alleles extend to other stresses. The incompatible allele of Med15 improved growth of yeast to chemicals that produce free radicals and the compatible allele of Med15 improved growth to reducing agents, caffeine, and hydroxyurea. Med15 directly interacts with Gcn4 and other TFs andin vitroform phase-separated droplets. This variation may reflect the positive and negative role that Med15 has in transcription. Genetic variation in transcriptional regulators can magnify differences in response to environmental changes, in contrast, genetic variation in a metabolic enzyme. These polymorphic control genes are master variators.
100 research rules of the game
Authors: Brennan Niamh M. 
Published: May 2019

PurposeThere are several basic, and at times minor, pedantic principles required to successfully publish in good-quality international peer-reviewed journals. These are what the author calls the “rules of the game”. Many are so basic, so taken-for-granted, tacit knowledge, that at times supervisors do not tell their students about them. The paper aims to discuss this issue.Design/methodology/approachThe author has assembled 100 research rules of the game from her work over many years with doctoral students and early career researchers. Each rule is accompanied by short advice. Additional citations are included directing readers to further resources on the 100 research rules of the game.FindingsThe paper documents 100 research rules of the game.Research limitations/implicationsThere are many other rules of the game not included in the author’s list of 100 research rules of the game.Originality/valueThis paper is a one-stop-shop brief introduction to the author’s 100 research rules of the game.
Learning @ work: informal learning strategies of German craft workers
Authors: Kortsch Timo , Schulte Eva-Maria , Kauffeld Simone 
Published: May 2019

Purpose
In competitive labor markets, promoting employees’ learning becomes a key challenge for companies. However, in small German craft companies, employee development is always connected with worries about employee turnover. This study aims to investigate the current informal learning strategies of craft workers and how they use the strategies, the effect of learning on employees’ internal and external marketability and beneficial workplace characteristics (autonomy, feedback).

Design/methodology/approach
An online prestudy (N = 131) explored current informal learning strategies. In the main study (N = 526), cluster analysis was applied to identify patterns of informal learning strategies. The relations of these patterns to workplace characteristics and marketability were investigated.

Findings
Four informal learning strategies were found (informal learning from oneself, from others, from other sources and from new media). Craft workers used combinations of the strategies (i.e. patterns): three learning patterns (balanced high, person-oriented and balanced low) differed in intensity and combination with the learning strategy use. More intense learning patterns were positively related to internal marketability but were not related to external marketability. Higher autonomy and feedback availability were related to higher learning engagement.

Research limitations/implications
Studies should have a broader view of informal learning strategies concerning different learning patterns. The use of new media is a learning strategy that might increase in the future.

Practical implications
Craft companies could promote different informal learning strategies without worries about employee turnover.

Originality/value
The study reveals how German small- and medium-sized enterprise employees use informal learning strategies in digitalized times and how human resources development can use informal learning strategies.
Board gender diversity and US corporate bonds
Authors: Oyotode-Adebile Renee M. , Raja Zubair Ali 
Published: May 2019

PurposeThe purpose of this paper is to examine the impact of board gender diversity on bond terms and bondholders’ returns.Design/methodology/approachThe authors perform pooled OLS regression, simultaneous regressions and propensity score matching to a panel data set of bond data for 319 US firms from 2007 to 2014.FindingsThe authors find that firms with gender-diverse boards have lower yields, higher ratings, larger issue size and shorter maturity. They also find that bondholders require fewer returns from firms with gender-diverse boards. However, the effect is more pronounced when women, constitutes at least 29.67 percent of the board.Originality/valueThis analysis supplements the findings that board gender diversity is essential for bondholders. It shows that bondholders should look at board gender diversity as a criterion to invest because bonds issued by firms with gender-diverse board have less risk. For practitioners, this study shows that more women participation on boards leads to a reduction in borrowing costs.
A cost minimisation model for system reliability allocation
Authors: Vaidya Omkarprasad S. , Ganapathy L. , Kumar Sushil 
Published: May 2019

Purpose
The purpose of this paper is to consider a nonlinear problem of minimizing the cost of providing reliable systems. The authors assume that the system consists of several components in series, and for each such component, the cost of the component increases exponentially with its reliability.

Design/methodology/approach
In order to solve this nonlinear optimization problem, the authors propose two approaches. The first approach is based on the concept of adjusting the reliability of a pair of components to minimize the cost of the system. The authors call this procedure as reliability adjustment routine (RAR). Proofs of optimality and convergence for the proposed model are also provided. The second approach solves the problem by using a Lagrangian multiplier. A procedure is developed to obtain the maximum step size to achieve the desired optimal solution in minimum iterations. Proposed approaches are efficient and give exact solutions.

Findings
Proposed methods enable a decision maker to allocate reliability to the components in series while minimizing the total cost of the system. The developed procedures are illustrated using a numerical example. Although an exponential relationship between the component cost and reliability is assumed, this can be extended to various other nonlinear distributions.

Originality/value
This cost optimization problem, subject to system component reliability values, assumes the near practical nonlinear pattern of cost vs reliability. Such problems are complex to solve. The authors provide a unique approach called RAR to solve such convoluted problems. The authors also provide an approach to solve such problems by using a Lagrangian multiplier method. Various proofs have been worked out to substantiate the work.
Preparedness for ASEAN Economic Community (AEC): a case study of Malaysian SME manufacturing sector
Authors: Ho Shelen W.H. 
Published: May 2019

PurposeThe ASEAN Economic Community (AEC) initiative poses both significant opportunities and difficult challenges to the Malaysian SME communities. This study aims to investigate the preparedness of the local SMEs to compete and exploit opportunities in a post-AEC era. The aim is to contribute to existing literature and knowledge base on SMEs’ practices in the ASEAN region. The manufacturing sector has been selected for study, and the research efforts focus on procurement competencies. SME procurement practices in the two largest manufacturing sub-sectors in Malaysia – resource-based (RB) and electrical and electronics (E&E) – are explored.Design/methodology/approachPreparedness for AEC is gauged from four key performance areas summarized from literature review. Six key research activities are also identified for each performance area from the findings of previous works. Data were collected using a survey instrument and face-to-face interviews. Research methodology is primarily qualitative with quantitative data for robustness check.FindingsThe findings reveal peculiar procurement practices in Malaysian SME manufacturers that have significant implications on their preparedness to compete effectively post-AEC. The findings also highlighted the key influences that have contributed to variations in Malaysian SMEs’ attitude toward trade liberalization challenges and business process improvements in comparison with practices in foreign firms and more advanced economies.Originality/valueTheoretical models have been adopted across business settings and environments by managers. This study reveals managerial practices in Malaysian SME manufacturers run counter to developed theories. The peculiarities and business attitudes of this resilient economic sector can be an important input to managerial decision-making when analyzing business activities in the region.
New product performance advantages for extending large, established fast moving consumer goods (FMCG) brands
Authors: Hoskins Jake David , Griffin Abbie 
Published: May 2019

Purpose
This paper aims to investigate how the current size and structure of a branded product portfolio impacts new product performance for fast-moving consumer goods (FMCG), testing the long-standing proposition that extending a firm’s brand and product portfolio too far is a dangerous proposition that may damage the market performance of the firm’s new product launches.

Design/methodology/approach
Aspects associated with brand size and structure that may impact new product performance are operationalized along two key dimensions: within-category (scale) and cross-category (scope). The impact of the brand’s scale and scope on the sales performance of newly commercialized products by the brand is empirically investigated in the context of FMCG. Over 2,000 new products launched in 2009 and 2010 across 31 food and non-food FMCG product categories in the USA are included in the regression-based analysis.

Findings
The authors find strong evidence that brands with broader within-category scale and cross-category scope overall are associated with more successful new product introductions, and that these influences generally are driven more by increased product trial than by repeat or persistence. The authors argue that the higher new product performance observed for more established and proliferated brands may be attributed to advantages of firm product development abilities and product acceptance by the marketplace.

Originality/value
The current results serve to temper the strong cautions set forth in much of the marketing literature about the dangers of overextending the firm’s brand and product portfolio. These results also suggest that future research should be conducted to further understand more nuanced implications of how best to grow the scale and scope of the firm’s brand and product portfolio.
Like a chameleon: the polychromatic virtue of dynamic brands
Authors: Lelis Catarina 
Published: May 2019

PurposeThe purpose of this paper is to identify the chromatic approaches in dynamic brand identities, describing and analysing new trends, patterns or shared strategies which seem to be taking place and renunciating the consistent use of corporate colours in some brands.Design/methodology/approachThe research consisted of a qualitative visual content analysis, based on the comparison and scrutiny of 50 dynamic visual identities, verifying the changes that their colours would undergo in their numerous forms of representation and the symbolic associations these would carry. This analysis was performed using three different studies.FindingsThe results show that colour in dynamic brands does not follow any consistent pattern regarding its application and none of the most common colour harmonies seem to be an obvious strategic preference.Practical implicationsThis research provides insights for brand managers to look at how this dynamic positioning can be successfully implemented without affecting recognition whilst establishing or maintaining customer loyalty, and for brand designers and marketers to clarify how brand guidelines will explain the usage of such colourful approaches.Originality/valueThis paper is a contribution to the knowledge of how a traditional visual element such as colour is being combined, deconstructed and reassembled in the context of modern visual identities. Three patterns are identified, and two of them draw attention to the apparent unnecessity of colour consistency and the way this may affect the relevance of colour in transmitting certain meanings.
Elevational cline in herbivore abundance driven by a monotonic increase in trophic‐level sensitivity to aridity
Authors: Nelson Annika S. , Symanski Cole T. , Hecking Matthew J. , Mooney Kailen A. 
Published: May 2019

Abstract

The abiotic environment drives species abundances and distributions both directly and indirectly through effects on multi‐trophic species interactions. However, few studies have documented the individual and combined consequences of these direct and indirect effects.

We studied an ant‐tended aphid along an elevational gradient, where lower elevations were more arid. Hypotheses of stronger species interactions at lower elevations and a greater sensitivity of higher trophic levels to climate led us to predict increased top‐down control of aphids by natural enemies (third trophic level) but even stronger protection from mutualist ants (fourth trophic level) with increasing aridity. As a result, we predicted that mutualism strength and aphid abundance would increase with aridity.

We documented patterns of aphid abundance and tested for both the direct and multi‐trophic indirect effects of aridity on aphid performance. To do so, we used both observational and manipulative methods across two years in replicate high‐ and low‐elevation valleys, where summer temperatures decreased by 3.7°C and precipitation increased by 27 mm/mo from low to high elevations.

Aphid colonies were 75% larger in the most (vs. least) arid sites, and this was best explained by changes in interactions with predators and ants. Aphids were unaffected by the direct effects of the abiotic environment or its indirect effects via host plant quality. In contrast, natural enemy effects increased with aridity; under ant exclusion, natural enemies had no effect on aphids in the least arid sites but depressed colony growth by 252% in the most arid sites. Ant activity also increased with aridity, with ants discovering more aphid colonies and experimental baits and allocating more foragers per aphid, although there was no effect of aridity on ant abundance or community composition. Correspondingly, the mutualist services provided by ants increased with aridity; ants provided no benefits to aphids in the least arid sites but doubled colony growth in the most arid sites.

In summary, an elevational cline in herbivore abundance was driven by a monotonic increase in trophic‐level sensitivity to aridity. These findings illustrate that predicting species responses to climate change will require a multi‐trophic perspective.
Interactive effects of grazing and global change factors on soil and ecosystem respiration in grassland ecosystems: A global synthesis
Authors: Zhou Guiyao , Luo Qin , Chen Yajie , Hu Jiaqi , He Miao , Gao Jing , Zhou Lingyan , Liu Huiying , Zhou Xuhui 
Published: May 2019

AbstractAs the key carbon (C) fluxes between biosphere and atmosphere, soil respiration (Rs) and ecosystem respiration (Re) play vital roles in regulating global C balance and climate‐biosphere feedback in the Earth system. Despite the fact that numerous manipulative studies and a few meta‐analyses have been conducted to examine the responses ofRsand its components (i.e. autotrophic [Ra] and heterotrophic respiration [Rh]) as well asReto grazing (G) or global change factors, the interactive effects between grazing and global change factors remain poorly understood.Here, we performed a comprehensive meta‐analysis of manipulative experiments with both grazing and global change factors to quantify their individual and interactive effects onRsand its components as well asRe.Our results showed that grazing and drought significantly decreasedRsby 12.35% and 20.95%, respectively, whereas warming (W), nitrogen addition (N) and increased precipitation (P) stimulated it by 2.12%, 5.49% and 13.44%, respectively. Similarly, grazing, warming, nitrogen addition and increased precipitation increasedReby 7.21%, 4.94%, 48.45% and 21.57%, respectively, while drought decreased it by 16.86%. However, the combinations of grazing with warming (GW), nitrogen addition (GN) and increased precipitation (GP) exhibited non‐significant effects onRs.More importantly, additive interactions between grazing and global change factors exhibited a substantial predominance onRs,Ra,RhandRerather than synergistic and antagonistic ones.Synthesis and applications. Our findings highlight the crucial importance of the interactive effects between grazing and global change factors on soil respiration (Rs) and ecosystem respiration (Re). Therefore, incorporating this key influence on ecosystem processes into Earth system models (ESMs) could better improve the prediction of climate‐biosphere feedbacks and develop sustainable strategies for grassland management in the Anthropocene.
Bacterial predation under changing viscosities
Authors: Sathyamoorthy Rajesh , Maoz Anat , Pasternak Zohar , Im Hansol , Huppert Amit , Kadouri Daniel , Jurkevitch Edouard 
Published: May 2019

SummaryBdellovibrio and like organisms (BALOs) are largely distributed in soils and in water bodies obligate predators of gram‐negative bacteria that can affect bacterial communities. Potential applications of BALOs include biomass reduction, their use against pathogenic bacteria in agriculture, and in medicine as an alternative against antibiotic‐resistant pathogens. Such different environments and uses mean that BALOs should be active under a range of viscosities. In this study, the predatory behaviour of two strains of the periplasmic predator B. bacteriovorus and of the epibiotic predator Micavibrio aeruginosavorus was examined in viscous polyvinylpyrrolidone (PVP) solutions at 28 and at 37°C, using fluorescent markers and plate counts to track predator growth and prey decay. We found that at high viscosities, although swimming speed was largely decreased, the three predators reduced prey to levels similar to those of non‐viscous suspensions, albeit with short delays. Prey motility and clumping did not affect the outcome. Strikingly, under low initial predator concentrations, predation dynamics were faster with increasing viscosity, an effect that dissipated with increasing predator concentrations. Changes in swimming patterns and in futile predator–predator encounters with viscosity, as revealed by path analysis under changing viscosities, along with possible PVP‐mediated crowding effects, may explain the observed phenomena.