bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancement of collagen deposition and cross-linking by coupling lysyl oxidase with bone morphogenetic protein-1 and its application in tissue engineering
Authors: Rosell-Garcia T. , Rodriguez-Pascual F. 
Published: July 2018

AbstractCultured cell-derived extracellular matrices (ECM)-based biomaterials exploit the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, standard cell culture conditions are far from ideal given the fact that the diluted microenvironment does not favor the production of ECM components, a circumstance particularly relevant for collagen. An incomplete conversion of procollagen by C-proteinase/bone morphogenetic protein 1 (BMP1) has been proposed to severely limit in vitro collagen deposition. BMP1 also catalyzes the proteolytic activation of the precursor of the collagen cross-linking enzyme, lysyl oxidase (LOX) to yield the active form, suggesting a deficit in cross-linking activity under standard conditions. We hypothesized that the implementation of fibroblast cultures with LOX and BMP1 may be an effective way to increase collagen deposition. To test it, we have generated stable cell lines overexpressing LOX and BMP1 and studied the effect of supernatants enriched in LOX and BMP1 on collagen synthesis and deposition from fibroblasts. Herein, we demonstrate that the supplementation with LOX and BMP1 strongly increased the deposition of collagen onto the insoluble matrix at the expense of the soluble fraction in the extracellular medium. Using decellularization protocols, we also show that fibroblast-derived matrices regulate adipogenic and osteogenic differentiation of human mesenchymal stem cells (MSC), and this effect was modulated by LOX/BMP1. Collectively, these data demonstrate that we have developed a convenient protocol to enhance the capacity of in vitro cell cultures to deposit collagen in the ECM, representing this approach a promising technology for application in tissue engineering.
Nitrogen-doping induces tunable magnetism in ReS2
Authors: Zhang Qin , Ren Zemian , Wu Nian , Wang Wenjie , Gao Yingjie , Zhang Qiqi , Shi Jing , Zhuang Lin , Sun Xiangnan , Fu Lei 
Published: July 2018

AbstractTransition metal dichalcogenides (TMDs) are promising for spintronic devices owing to their spin-orbit coupling and loss of inversion symmetry. However, further development was obstructed by their intrinsic nonmagnetic property. Doping TMDs with non-metal light atoms has been predicted to be a good option to induce unexpected magnetic properties which remain rarely explored. Here, we utilize nitrogen doping to introduce magnetic domains into anisotropic ReS2, giving rise to a transition from nonmagnetic to tunable magnetic ordering. Both of the experimental and computational results confirmed that the N-doping in ReS2 prefers to take place at the edge site than in-plane site. With controlled doping concentration, it exhibits a unique ferromagnetic-antiferromagnetic (FM-AFM) coupling. Assisted by theoretical calculations, we demonstrated that FM-AFM coupling presents a strong link to doping contents and doping sites. Wherein, the FM ordering mostly comes from N atoms and the AFM ordering originate from Re atoms. At the N-doping content of 4.24%, the saturated magnetization of N-doped ReS2 reached the largest value of 2.1 emu g−1 at 2 K. Further altering the content to 6.64%, the saturated magnetization of N-doped ReS2 decreases, but exhibits a distinct exchange bias (EB) phenomenon of around 200 Oe. With controlled N-doping concentrations, the intrinsic spin in ReS2 could be well altered and resulted in distinct magnetism, presenting tremendous potential for spintronic devices in information storage.
Risk factors for onset of delirium after neck of femur fracture surgery: a prospective observational study
Authors: Tahir Muaaz , Malik Shahbaz S , Ahmed Usman , Kozdryk Jakub , Naqvi Syeda Huma , Malik Atul 
Published: July 2018

Background: Delirium is a common complication after surgery in the elderly that leads to increased length of stay and other adverse outcomes. The aim of this study was to better understand the exact causes of post-operative delirium in patients undergoing surgery for neck of femur (NOF) fractures.Methods: We performed a prospective cohort study of 381 consecutive patients undergoing surgery for NOF fractures at a single institution. Baseline cognitive status and risk factors were recorded on admission. Post-operative cognitive status was assessed at regular intervals until discharge. Binary logistic regression was performed to identify independent predictors of delirium.Results: Patients who developed post-operative delirium (n = 70) were significantly older (average age 83 vs. 78,p = 0.019) and more likely to be female (79% vs. 67%,p = 0.062) than non-affected patients. The presence of delirium was associated with increased length of stay (13 vs. 10 days,p = 0.001) and 1-year mortality (25.7% vs. 15%p = 0.03). Independent predictors of delirium included age ≥65 years (Odds Ratio = 5.8), presence of anaemia (OR = 2.9), hypoxia (OR = 2.86), cardiac disease (OR = 2.8), Chronic Obstructive Pulmonary Disease (OR = 2.5), new onset electrolyte imbalance (OR = 2.2) and renal failure (OR = 1.9).Conclusion: Overall analysis demonstrated an increased incidence of delirium in older females with greater comorbid conditions. It was also found to be associated with increased morbidity and mortality. We recommend clinicians put greater effort into recognising risk factors of delirium and diagnosing it in a timely manner to mitigate its effects.
Development of a computed cleansing score to assess quality of bowel preparation in colon capsule endoscopy
Authors: Becq Aymeric , Histace Aymeric , Camus Marine , Nion-Larmurier Isabelle , Abou Ali Einas , Pietri Olivia , Romain Olivier , Chaput Ulriikka , Li Cynthia , Marteau Philippe , Florent Christian , Dray Xavier 
Published: July 2018

Abstract
Background and study aims Colon capsule endoscopy (CCE) does not possess an objective and reliable scoring system to assess the quality of visualization of the colon mucosa. The aim of this study was to establish a colonic computed assessment of cleansing (CAC) score able to discriminate “adequately cleansed” from “inadequately cleansed” CCE still frames.
Patients and methods Twelve normal and complete CCEs, using the Pillcam Colon 2 system (Medtronic, Minnesota, United States), were prospectively selected amongst a database. A CAC score, defined as the ratio of color intensities red over green (R/G ratio), and red over brown (R/(R + G) ratio) was calculated for each extracted colonic frame. After sorting and random selection, two sets of still frames representative of the range of these ratios were obtained. These images were analyzed twice in random order by two experienced CCE readers who were blinded to the CAC scores. A receiver operating characteristic (ROC) curve was forged for both types of ratios and a threshold established, yielding the highest diagnostic performance in terms of adequate cleansing assessment.
Results Four-hundred-and-eight frames were extracted. Regarding the R/G ratio, a threshold value of 1.55 was calculated, with a sensitivity of 86.5 % and a specificity of 77.7 %. Regarding the R/(R + G) ratio, a threshold value of 0.58 was calculated with a sensitivity of 95.5 % and a specificity of 62.9 %.
Conclusion The two proposed CAC scores based on the ratio of color intensities come with high sensitivities for discriminating between “adequately cleansed” and “inadequately cleansed” CCE still frames, but they lack specificity. Further refinement, with implementation of additional image parameters, is warranted.
Use of anticoagulants increases risk of bleeding after colorectal endoscopic submucosal dissection
Authors: Yamashita Ken , Oka Shiro , Tanaka Shinji , Boda Kazuki , Hirano Daiki , Sumimoto Kyoku , Mizumoto Takeshi , Ninomiya Yuki , Tamaru Yuzuru , Shigita Kenjiro , Hayashi Nana , Sanomura Yoji , Chayama Kazuaki 
Published: July 2018

Abstract
Background and study aims Japanese guidelines for gastroenterological endoscopy have recommended temporary withdrawal of anticoagulants (warfarin, direct oral anticoagulants [DOAC], or heparin) to prevent hemorrhagic complications during endoscopic submucosal dissection (ESD) for colorectal neoplasias (CRNs). However, serious thrombosis might occur during temporary withdrawal of anticoagulants. The current study aimed to evaluate outcomes with anticoagulants in patients undergoing ESD for CRNs.
Patients and methods This study was a single-institution retrospective cohort study based on clinical records. We assessed 650 consecutive patients with 698 CRNs who underwent ESD at Hiroshima University Hospital between December 2010 and June 2016. The patients were divided into three groups: the warfarin group (19 patients with 19 CRNs), DOAC group (7 patients with 9 CRNs), and no-antithrombotics group (624 patients with 670 CRNs). We replaced warfarin with heparin 3 to 5 days before endoscopy. Although DOAC was suspended on the morning of endoscopy, we did not replace heparin.
Results Bleeding after the procedure occurred in 26.3 % (5/19), 22.0 % (2/9), and 2.7 % (18/670) of patients in the warfarin, DOAC, and no-antithrombotics groups, respectively. In the warfarin group, four patients who bled after the procedure took not only warfarin but also other antiplatelets. En bloc resection rates were 94.7 % (18/19), 100 % (9/9), and 96.6 % (647/670) in the warfarin, DOAC, and no-antithrombotics groups, respectively. No patients experienced ischemic events in the perioperative period.
Conclusions Among patients undergoing ESD for CRNs, risk of bleeding was higher among patients who took anticoagulants than among those who did not. In particular, careful attention to patients who took antiplatelets in addition to warfarin before ESD for CRNs is warranted.
EUS-guided sampling with 25G biopsy needle as a rescue strategy for diagnosis of small subepithelial lesions of the upper gastrointestinal tract
Authors: Antonini Filippo , Giorgini Sara , Fuccio Lorenzo , Angelelli Lucia , Macarri Giampiero 
Published: July 2018

Abstract
Background and study aims This study was designed to evaluate the impact of additional tissue obtained with endoscopic ultrasound (EUS)-guided 25-gauge core biopsy needle (25G-PC) following an unsuccessful fine-needle biopsy (FNB) performed with larger-bore needles for the characterization of gastrointestinal subepithelial lesions (GI-SELs).
Patients and methods We prospectively collected and retrospectively analyzed information in our database from January 2013 to June 2017 for all patients with GI-SELs who received a EUS-guided FNB (EUS-FNB) with 25G-PC during the same procedure after failure of biopsy performed with larger-bore needle. Diagnostic yield, diagnostic accuracy and procedural complications were evaluated.
Results Sixteen patients were included in this study, 10 men and 6 women, median age 67.8 (range 43 to 76 years). Five patients were found to have a SEL localized in the distal duodenum, five in the gastric antrum, two in the gastric fundus and four in the gastric body. The mean size of the lesions was 20.5 mm (range 18 – 24 mm). EUS-FNB with 25G-PC enabled final diagnosis in nine patients (56.2 %). Regarding the subgroup of duodenal lesions, the procedure was successful in four of five (80 %). Final diagnoses with EUS-guided sampling were GIST (n = 6), leiomyoma (n = 2) and metastatic ovarian carcinoma (n = 1). No procedure-related complications were recorded.
Conclusion In patients with small GI-SELs, additional tissue obtained with 25G-PC could represents a “rescue” strategy after an unsuccessful procedure with larger-bore needles, especially when lesions are localized in the distal duodenum.
Endoscopic management of difficult benign biliary and pancreatic strictures using a wire-guided cystotome: experience with 25 cases
Authors: Puri Rajesh , Bhatia Sumit , Bansal Rinkesh , Sud Randhir 
Published: July 2018

Abstract
Background and study aims Endoscopic management using standard accessories is the preferred modality for treatment of benign biliary strictures. However, with difficult strictures, there is frequently failure of endoscopic therapy. We are reporting our experience regarding use of a standard diathermic dilator (cystotome) to aid in stricture dilatation and stent placement in patients with difficult strictures.
Patients and methods Data were analyzed from January 2014 to January 2017 at a single tertiary care center in North India. Total 25 patients were included.
Results The mean age was 45 years (varying from 38 – 55 years). Of the 25 patients with difficult strictures, 14 (56 %) were male and 11 (44 %) were female. Further, of these, 19 had biliary and 6 had pancreatic strictures. The average time of diathermic current application was 3.5 seconds (ranging 3 – 5 seconds). Technical and clinical success were achieved in 100 % of cases. None of the patients had any procedure-related (early or delayed) major complications.
Conclusion The cystotome is an extremely safe and useful accessory in benign biliary and pancreatic strictures, whereas conventional methods to negotiate stricture have failed.
Relationships Between Olympic Weightlifting Exercises, Peak Power of the Upper and Lower Limb, Muscle Volume and Throwing Ball Velocity in Elite Male Handball Players
Authors: Hermassi Souhail , Delank Karl Stefan , Fieseler Georg , Bartels Thomas , Chelly Mohamed Souhaiel , Khalifa Riadh , Laudner Kevin , Schulze Stephan , Schwesig René 
Published: July 2018

Abstract
Background This study aimed to investigate relationships between peak power (PP) as measured by upper limb (PPUL) and lower limb (PPLL) force velocity tests, maximal upper limb force assessed by clean and jerk (1RMCJ) and snatch (1RMSE) exercises, estimates of local muscle volume and throwing ball velocity.
Methods Thirty elite male handball players volunteered for the investigation (age: 20.3 ± 2.1 years; body mass: 82.5 ± 10.5 kg; height: 1.85 ± 0,07 %; body fat: 13.1 ± 2.1 %). Lower and upper limb force velocity tests were performed on appropriately modified forms of a Monark cycle ergometer with measurement of PPUL and PPLL, and the corresponding respective maximal forces (F0UL and F0LL) and velocities (V0UL and V0LL). Snatched (SN) and clean and jerk (CJ) exercises were performed to one repetition maximum (1RM). Handball throwing velocity was measured with jump shot (JS) without run-up (TW) and 3 steps shot (T3 step). Muscle volumes of the upper and lower limbs were estimated with a standard anthropometric kit.
Results The 1RM CJ proved to be the most important predictor for throwing velocity. All types of throwing showed a high correlation with this parameter (JS: r = 0.75; TW: r = 0.62; T3 step: r = 0.66). The highest relation was detected between jump shot and 1RM snatch technique (r = 0.82). The PPUL muscle volume correlated highly with PPUL absolute power (r = 0.70). In contrast, we did not find any comparable relations for the lower limb (muscle volume vs. PPUL absolute power: r = 0.07).
Conclusions Our results highlight the contribution of both lower and upper limbs to handball throwing velocity, suggesting the need for coaches to include upper and lower limb strength weightlifting exercises and power programs when improving the throwing velocity of handball players.
Demographic Characteristics and Functional Performance of the Kidneys and Hearts of Patients with Acute Tramadol Toxicity
Authors: Mohammadpour Abbas , Ashkezari Mahmood , Farahmand Behrokh , Shokrzadeh Mohammad 
Published: July 2018

Abstract
Background Tramadol overdose is disproportionately more common in Iran, and in recent years, it has become one of the most common causes of poisoning admissions to emergency departments in this country. Tramadol is a synthetic analogue of codeine and a weak opioid receptor (μ) agonist that can cause seizures even in commonly used doses.
Aims This study aimed to examine the relationship between seizure and plasma tramadol concentration in patients with tramadol poisoning who referred to one of the hospitals in Ghaemshahr, Iran.
Study Design This research is an analytical cross-sectional study.
Methods A total of 121 tramadol users (non-seizure group=61 and seizure group=60) were admitted to hospital. Demographic characteristics and clinical findings were collected by a questionnaire. Plasma was harvested after separation from blood cells and quantified using the HPLC method. Biochemical parameters, including urea, creatinine, troponin I, and creatine phosphokinase (CPK–MB) were determined by spectrophotometry. This study was a single blind design study.
Results The mean age of participants was 25 years. Ninety-five participants were classified as smokers. The mean serum concentrations of tramadol in subjects with seizures and those without seizures were 491.90 μg/ml and 374.42 μg/ml (p=0.211), respectively. Average concentrations of biochemical parameters in the seizure group were 53.33 (9.38) µg/ml urea, 1.71 (0.29) µg/ml Cr, 6.53 (2.89) µg/ml TPI, and 58.23 (22.20) µg/ml CPK- MB. Average concentrations of biochemical parameters were significantly higher in the seizure group than in the non-seizure group (p<0.001).
Conclusion Tramadol-induced seizures were not found to be related to age, gender, or dosage. This complication can lead to cardiac and renal complications in individuals on tramadol experiencing seizures. This result indicates that stricter restrictions should be imposed on the distribution and administration of the drug tramadol.
Fluctuations in Plasma Levels of Thrombomodulin in Patients with DIC
Authors: Amano Kagehiro , Tateyama Masaki , Inaba Hiroshi , Fukutake Katsuyuki , Fujimaki Michio 
Published: July 2018

SummaryPlasma thrombomodulin (TM) has attracted considerable attention as a marker of endothelial cell membrane injury. We examined fluctuations in plasma TM levels in patients receiving therapy for the disseminated intravascular coagulation syndrome (DIC) using an enzyme immunoassay. Sixty healthy controls and 18 patients with DIC were studied.The mean ± SD of the TM values initially measured immediately after the onset of DIC was 42.00 ± 20.85 ng/ml, which was markedly increased as compared with the control value of 15.36 ±4.85 ng/ml (p <0.001).Fluctuations in the TM levels over time were studied after dividing the patients according to the presence or absence of improvement in the underlying disease and improvement or lack thereof in the coagulation findings. Group I showed improvement in both categories, Group II showed improvement only in the latter, and Group III showed no improvement in either category.In Group I, the mean ± SD of initial measured TM levels was 37.02 ±10.12 ng/ml and the mean of final values decreased to 58.9% of the initial value. This decrease was significant by paired Student’s t-test (p <0.01). The initial value in Group II was 45.86 ± 18.86 ng/ml and the final values increased to 117.0% of the initial values, this difference was not significant. The initial value in Group III was 44.48 ±21.53 ng/ml and the final values increased to 143.4% of the former. This increase was significant by paired Student’s t-test (p <0.05). The difference in % fluctuations between Group I and Group III was significant by Wilcoxon’s test (p <0.01).These results suggest that the measurement of plasma TM can be useful in the management of DIC.
Comparison of the Inhibitory Effects of the TXA2 Receptor Antagonist, Vapiprost, and Other Antiplatelet Drugs on Arterial Thrombosis in Rats: Possible Role of TXA2
Authors: Takiguchi Yoshiharu , Wada Kouichirou , Nakashima Mitsuyoshi 
Published: July 2018

SummaryThe antithrombotic effect of the thromboxane A2 receptor antagonist, vapiprost, was compared with those of other antiplatelet drugs using an arterial thrombosis model which utilized photochemical reaction in the rat femoral artery. Vapiprost prolonged the time required to occlude the artery with thrombus and inhibited collagen-induced rat platelet aggregation in whole blood ex vivo, in a dose-dependent manner. The potency ranking of antithrombotic effect was vapiprost > ketanserin (serotonin 5-HT2 receptor antagonist) >> ticlopidine (inhibitor of ADP-induced platelet aggregation) = dipyridamole (adenosine uptake inhibitor) >aspirin (cyclooxygenase inhibitor). On the other hand, the ranking of antiplatelet effect was ticlopidine ≥vapiprost ≥aspirin. Ketanserin and dipyridamole were ineffective. Relative to their antiplatelet effect, vapiprost and ketanserin had powerful antithrombotic effects. It is possible that the potent antithrombotic effects of vapiprost and ketanserin in vivo reflect the ability of these drugs to inhibit mediator-induced vascular contractions in addition to platelet aggregation. The results of the present study also suggest that TXA2 may play an important role in thrombogenesis in rats.
A Novel Rat Model of Thrombogenicity: Its Use in Evaluation of Prothrombin Complex Concentrates and High Purity Factor IX Concentrates
Authors: McLaughlin L F , Drummond O , MacGregor I R 
Published: July 2018

SummaryA non-stasis rodent model of thrombogenicity has been used for dose-ranging studies with a conventional prothrombin complex concentrate (PCC) and to evaluate high purity factor IX concentrates from different manufacturers. Fibrin monomer (soluble fibrin) and fibrinopeptide A (FPA) were monitored before and after infusion of test solution. FPA was found to be the more sensitive and reproducible indicator of thrombogenicity and exhibited a dose-related elevation after infusion of the PCC at doses of between 100–300 IU/kg. In contrast the amounts of FPA generated after 300 IU/kg of the high purity factor IX products were similar to control infusions of albumin.
Stored Platelets Release Nucleotides as Inhibitors of Platelet Function
Authors: Fijnheer Rob , Boomgaard Martine N , van den Eertwegh Alfons J M , Homburg Christa H E , Gouwerok Casparus W N , Veldman Henk A , Roos Dirk , de Korte Dirk 
Published: July 2018

SummaryIt is well known that the function of platelets decreases progressively during storage of platelet concentrates at room temperature. To investigate this phenomenon in more detail, we have resuspended platelets that had been stored for 24 h or 72 h in fresh plasma, and we have measured the aggregation response and the ATP secretion. Conversely, the effect of plasma in which platelet concentrates (PC) had been stored for 24 h or 72 h, was tested on fresh platelets. Both the aggregation response to collagen and ADP and the collagen-induced ATP secretion of stored platelets partially recovered after incubation with fresh plasma (p <0.05). The same parameters measured with fresh platelets incubated in stored PC-plasma were found to be significantly reduced in comparison with the response of fresh platelets in fresh plasma (p <0.05). Finally, platelets were stored in a plasma-free medium, suitable for platelet storage and the supernatant was tested. This supernatant inhibited the function of fresh platelets in a storage time-dependent fashion. Boiling of these supernatants did not change the inhibiting capacities, whereas filtration over active charcoal did. Analysis of this supernatant revealed AMP and diadenosine tetraphosphate, which both inhibit platelet function.These data show that stored platelets release nucleotides that inhibit platelet function in a reversible manner. This phenomenon may contribute to the decrease of platelet function during storage and the recovery of platelet function after transfusion.