bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of a role for an E6-like 1 gene in early pollen-stigma interactions in Arabidopsis thaliana
Authors: Doucet Jennifer , Truong Christina , Frank-Webb Elizabeth , Lee Hyun Kyung , Daneva Anna , Gao Zhen , Nowack Moritz K. , Goring Daphne R. 
Published: April 2019

AbstractIn Arabidopsis, successful pollen-stigma interactions are dependent on rapid recognition of compatible pollen by the stigmatic papillae located on the surface of the pistil and the subsequent regulation of pollen hydration and germination, and followed by the growth of pollen tubes through the stigma surface. Here we have described the function of a novel gene, E6-like 1 (E6L1), that was identified through the analysis of transcriptome datasets, as one of highest-expressed genes in the stigma, and furthermore, its expression was largely restricted to the stigma and trichomes. The first E6 gene was initially identified as a highly-expressed gene during cotton fiber development, and related E6-like predicted proteins are found throughout the Angiosperms. To date, no orthologous genes have been assigned a biological function. Both the Arabidopsis E6L1 and cotton E6 proteins are predicted to be secreted, and this was confirmed using an E6L1:RFP fusion construct. To further investigate E6L1’s function, one T-DNA and two independent CRISPR-generated mutants were analyzed for compatible pollen-stigma interactions, and pollen hydration, pollen adhesion and seed set were mildly impaired for the e6l1 mutants. This work identifies E6L1 as a novel stigmatic factor that plays a role during the early post-pollination stages in Arabidopsis.Key MessageWe describe a function for a novel Arabidopsis gene, E6-like 1 (E6L1), that was identified as a highly-expressed gene in the stigma and plays a role in early post-pollination stages.
Multi-scale Computational Modeling of Tubulin-Tubulin Lateral Interaction
Authors: Hemmat M. , Castle B.T. , Sachs J.N. , Odde D.J. 
Published: April 2019

AbstractMicrotubules are multi-stranded polymers in eukaryotic cells that support key cellular functions such as chromosome segregation, motor-based cargo transport, and maintenance of cell polarity. Microtubules self-assemble via “dynamic instability,” where the dynamic plus ends switch stochastically between alternating phases of polymerization and depolymerization. A key question in the field is what are the atomistic origins of this switching, i.e. what is different between the GTP- and GDP-tubulin states that enables microtubule growth and shortening, respectively? More generally, a major challenge in biology is how to connect theoretical frameworks across length-time scales, from atoms to cellular behavior. In this study, we describe a multi-scale model by linking atomistic molecular dynamics (MD), molecular Brownian dynamics (BD), and cellular-level thermo-kinetic (TK) modeling of microtubules. Here we investigated the underlying interaction energy landscape when tubulin dimers associate laterally by performing all-atom molecular dynamics simulations. We found that the lateral free energy is not significantly different among three nucleotide states of tubulin, GTP, GDP, and GMPCPP, and is estimated to be ≅−11 kBT. Furthermore, using MD potential energy in our BD simulations of tubulin dimers in solution confirms that the lateral bond is weak on its own with a mean lifetime of ~0.1 μs, implying that the longitudinal bond is required for microtubule assembly. We conclude that nucleotide-dependent lateral bond strength is not the key mediator microtubule dynamic instability, implying that GTP acts elsewhere to exert its stabilizing influence on microtubule polymer. Furthermore the estimated bond strength is well-aligned with earlier estimates based on thermokinetic (TK) modeling and light microscopy measurements (VanBuren et al., PNAS, 2002). Thus, we have computationally connected atomistic level structural information, obtained by cryo-electron microscopy, to cellular scale microtubule assembly dynamics using a combination of MD, BD, and TK models to bridge from Ångstroms to micrometers and from femtoseconds to minutes.
Individual responses to competing accountability pressures in hybrid organisations
Authors: Gebreiter Florian , Hidayah Nunung Nurul 
Published: April 2019

Purpose
The purpose of this paper is to examine conflicting institutional demands on individual frontline employees in hybrid public sector organisations. Specifically, it examines the competing accountability pressures professional and commercial logics exerted on academics at a business school, how individual lecturers responded to such pressures, and what drove these responses.

Design/methodology/approach
The paper draws on a case study of an English business school and is informed by the literatures on institutional logics and hybrid organisations.

Findings
The paper shows that the co-existence of professional and commercial logics at the case organisation exerted competing accountability pressures on lecturers. It moreover shows that sometimes deliberately and purposefully, sometimes ad hoc or even coincidentally, lecturers drew on a wide range of responses to these conflicting pressures, including compliance, defiance, combination and compartmentalisation.

Originality/value
The paper sheds light on individual level responses to competing institutional logics and associated accountability pressures, as well as on their drivers. It also highlights the drawbacks of user, customer or citizen accountability mechanisms, showing that a strong emphasis on them in knowledge-intensive public organisations can have severe dysfunctional effects.
Long-term prediction on atmospheric corrosion data series of carbon steel in China based on NGBM(1,1) model and genetic algorithm
Authors: Zhi Yuanjie , Fu Dongmei , Yang Tao , Zhang Dawei , Li Xiaogang , Pei Zibo 
Published: April 2019

PurposeThis study aims to achieve long-term prediction on a specific monotonic data series of atmospheric corrosion rate vs time.Design/methodology/approachThis paper presents a new method, used to the collected corrosion data of carbon steel provided by the China Gateway to Corrosion and Protection, that combines non-linear gray Bernoulli model (NGBM(1,1) with genetic algorithm to attain the purpose of this study.FindingsResults of the experiments showed that the present study’s method is more accurate than other algorithms. In particular, the mean absolute percentage error (MAPE) and the root mean square error (RMSE) of the proposed method in data sets are 9.15 per cent and 1.23 µm/a, respectively. Furthermore, this study illustrates that model parameter can be used to evaluate the similarity of curve tendency between two carbon steel data sets.Originality/valueCorrosion data are part of a typical small-sample data set, and these also belong to a gray system because corrosion has a clear outcome and an uncertainly occurrence mechanism. In this work, a new gray forecast model was proposed to achieve the goal of long-term prediction of carbon steel in China.
Dyslexia and policing
Authors: Macdonald Stephen J. , Cosgrove Faye 
Published: April 2019

PurposeThe purpose of this paper is to explore the experiences of officers/civilians with dyslexia serving in the police service in England and Wales. Although there has been a growing body of research which has analysed the experiences of offenders and victims with dyslexia, there have been few studies focusing on the experiences of police officers/civilians with this condition. This study employs the social model of disability to conceptualise the experiences of these police officers/civilians from a disability rights perspective.Design/methodology/approachThis applies a quantitative methodology to analyse data on disabling environments experienced by officers/civilians serving in a police service situated in the North of England. The paper collected data from 56 police employees previously diagnosed with dyslexia.FindingsThe findings reveal that a significant number of officers were reluctant to disclose that they had dyslexia to their police service. The choice to disclose was a key concern for officers/civilians, as this was directly linked to their experiences of stigmatisation, as well as the risk of their competences being questioned at work. The analysis presents evidence that, although officers/civilians have legal protections under the Equality Act 2010 (c15) in the UK, very few had experienced any form of “reasonable adjustment” in the workplace.Originality/valueDrawing on the social model of disability, the paper concludes that the police service must improve access to reasonable adjustment, for example, through the use of assistive technologies, to create a more inclusive and supportive working environment for their employees.
Improving the soft skills of engineering undergraduates in Malaysia through problem-based approaches and e-learning applications
Authors: Deep Sadia , Salleh Berhannudin Mohd , Othman Hussain 
Published: April 2019

Purpose
Various studies around the globe indicate lack of soft skills in engineering students, graduating from universities, although the bulk of studies in various disciplines indicate an improvement in various skills through implementation of a problem-based learning (PBL) approach. The purpose of this paper is therefore to identify and determine the effects of PBL in improving and developing soft skills, conflict resolution traits and aspects of enhancing group learning in undergraduates.

Design/methodology/approach
A study with mixed methods was used, involving questionnaire, observation and document analysis. A total of 57 students from different faculties participated in the semester-long study. Pre-tests and post-tests were administered at the beginning and end of the study. The researcher attended all the classes to observe systematically the teaching-learning process and its outcomes.

Findings
The findings revealed that PBL has a significant effect on improving students’ soft skills and enhancing group learning, including overcoming communication conflicts.

Practical implications
Practical implications of the study involve the enhancement of generic skills of the engineering graduates with the student-centered and interactive methodologies like PBL and e-learning applications.

Originality/value
This research endeavor stands unique due to its usage of e-learning tools in the PBL classroom learning such as Schoology, Padlet, videos, internet surfing. Another unique feature of this research is the holistic nature of the study, i.e. the measurement of a number of soft skills, traits and group learning skills that are not focused in other studies of engineering.
Hygrothermal simulation of cathedral ceiling roofs with ventilated roofing tiles
Authors: Kölsch Philipp 
Published: April 2019

Purpose
The surface temperature of the sub-roof beneath the ventilation layer and the tiles is one of the most important factors for the hygrothermal performance of pitched roofs. The air layer between tiles and sub-roof and the air exchange with the outdoor air influence the heat transfer and therefore affect the moisture level inside the roof construction. The paper aims to discuss these issues.

Design/methodology/approach
This paper provides the results of a research project performed at Fraunhofer-Institute for Building Physics, based on field test results. The investigations analyze the thermal behavior of different vented and ventilated roof constructions.

Findings
It was found that for a detailed model with roof cladding and ventilated air layer normally too many parameters are unknown. For that reason a simplified approach was set up, especially to consider the radiation exchange between the tiles and the underlay as well as the effects of the ventilation.

Originality/value
Now, effective surface transfer parameters can substitute both cladding and air layer in the simulation, while the approach still provides a high accordance with the measured values. The paper provides characteristic values for different roofing situations to simulate ventilated roofs by means of hygrothermal simulation in a simplified way.
Co-innovation toolbox for demand-supply chain synchronisation
Authors: Salmela Erno , Huiskonen Janne 
Published: April 2019

Purpose
The purpose of this paper is to promote decision-making structures between the customer and the supplier in a highly uncertain environment. This phenomenon of demand-supply chain synchronisation includes sharing of high-quality and timely demand and supply information in order to improve the quality and speed of decision-making.

Design/methodology/approach
The study was carried out as an abductive case study, which started from empirical observations that did not match the prior theoretical framework. Through abductive reasoning and empirical experiments, the prior framework was extended to a new synchronisation model and tools that better accommodate the observed need.

Findings
A new co-innovation toolbox was developed to create common understanding of demand-supply chain synchronisation between the customer and the supplier. The toolbox includes Demand Visibility Point-Demand Penetration Point, Supply Visibility Point–Supply Penetration Point and Integrative Synchronisation tools.

Research limitations/implications
The study extends the current models and tools of demand-supply chain synchronisation. With the new toolbox, the development needs of decision-making structures can be identified more comprehensively than with the current tools.

Practical implications
The developed visual toolbox helps partners create a common understanding of problems and development possibilities in demand-supply chain synchronisation in a highly uncertain environment. Common understanding is a starting point for changing decision-making structures to improve the overall performance of a demand-supply chain.

Originality/value
The new toolbox is both more comprehensive and more detailed than the previous tools.
An evaluation of used objects in bank TV commercials in Ramadan
Authors: Aydin Oguzhan , Hasiloglu Selcuk Burak 
Published: April 2019

Purpose
The purpose of this study is to find the most effective objects used in Ramadan TV ads of banks operated in Turkey and to determine superiority of the objects among these ads.

Design/methodology/approach
This research examines used objects in bank TV advertisements for the month of Ramadan by applying content analysis and fuzzy logic method.

Findings
The most prominent of the findings, as a result of the research, are kids and the elder objects in bank TV commercials. The research findings also reveal the importance of religious objects of Ramadan ads.

Research limitations/implications
The limitation of this research is non-generalization of the results because of sample size. Future research could explore the impact of objects used in TV ads on consumers with a larger sample size.

Practical implications
The banks are eager to look for opportunities by launching campaigns during the period of Ramadan. Special occasions such as religious holidays are seen as a new market that should not to be missed by the finance sector, and therefore, the number of bank TV commercials increase dramatically in this term. However, it is important for banks to know which objects are more effective. In this study, it is revealed that objects used in TV ads are more effective.

Originality/value
This is the first study to examine bank TV commercials during the period of Ramadan. It is also important to reflect Turkish consumers’ perspective and banks’ promotion activities in a Muslim country.
Intersectional history: exploring intersectionality over time
Authors: Shaffner Ellen C. , Mills Albert J. , Helms Mills Jean 
Published: April 2019

PurposeThis paper aims to outline the possibilities of intersectional history as a novel method for management history. Intersectional history combines intersectionality and the study of the past to examine discrimination in organizations over time. This paper explores the need for intersectional work in management history, outlines the vision for intersectional history and provides a brief example analyzing the treatment of Australian Aboriginal people in a historical account of Qantas Airways.Design/methodology/approachThis paper contends that intersectionality is a discursive practice, and it adopts a relational approach to the study of the past to inform the method. This paper focuses on the social construction of identities and the enduring nature of traces of the powerful in organizations over time.FindingsThe example of Qantas Airways demonstrates that intersectional history can be used to interrogate powerful traces of the past to reveal novel insights about marginalized peoples over time.Originality/valueIntersectional history is a specific and reflexive method that allows for the surfacing of identity-based marginalization over time. The paper’s concentration on identity as socially constructed allows a particular focus on notions or representations of the marginalized in traces of the past. These traces may otherwise mask the existence and importance of marginalized groups in organizations’ dominant histories.
Evidence-based asset management applied to maintenance function control
Authors: Herrera Galán Michael 
Published: April 2019

Purpose
Every day a large volume of information is generated in the maintenance department relating to its business assets whose detailed analysis has a direct impact on the effectiveness of the company. Taking into account the aforementioned, it is necessary to develop and implement a set of indicators that allow a practical evaluation of the maintenance function in any firm. Therefore, the purpose of this paper is to evaluate the maintenance function through a thorough analysis of historical data of a biotechnology sector firm.

Design/methodology/approach
The method used to evaluate the maintenance function of a Cuban company is based on the quantification of a set of indicators, represented graphically. The data processed in this paper were obtained through an informatics application, designed to computerize the maintenance function in the firm. This application is validated and is part of the company quality management system, which is audited every two years by the national regulatory authority CECMED (Centro para el Control Estatal de Medicamentos, Equipos y Dispositivos Médicos de Cuba, by its Spanish acronym), for issuing the certificates of sanitary license and good manufacturing practices.

Findings
Evidence-based control alternatives were used to evaluate the adequacy of the maintenance function in a biotechnology company. The results demonstrate graphically the maintenance operations of the National Center for Scientific Research, production department, during a period from January 2013 to December 2017. Finally, based on the analysis, it was discovered that the performance of the maintenance department was inadequate and had poor effectiveness, and a new maintenance strategy was established to be followed for the next quinquennium.

Originality/value
The research proposal provides information on how to evaluate the proactive and reactive maintenance actions through graphical indicators. The results obtained together with traditional maintenance indicators such as availability, maintainability and reliability could be interesting to technicians or engineers who decide to evaluate directly the effectiveness of a maintenance department.
Understanding non-private label consumers’ switching intention in emerging market
Authors: Aw Eugene Cheng-Xi , Chong Han Xi 
Published: April 2019

PurposeThe purpose of this paper is to propose and empirically test a research model encompassing marketing mix activities as antecedents of non-private label consumers’ switching intention, particularly from an emerging market perspective. The study also aims to test the moderating role of general neophobia and gender.Design/methodology/approachFocusing on non-private label consumers, the study analyzed a total of 211 questionnaire responses. Partial least squares structural equation modeling was used to test the research model.FindingsThe results suggested that marketing mix activities, particularly advertising, in-store communication, and monetary promotion positively influenced private label brand attitude. Attitude positively influenced switching intention. The proposed moderating effects of general neophobia and gender in the relationship between private label brand attitude and switching intention were supported.Originality/valueThis study provides empirical evidence to the effects of marketing practices on private label brand attitude from an emerging market perspective, complementing previous research which largely focused on developed market. The findings offer managerial ideas in targeting non-private label consumers. The test of moderating variables expands the understanding on attitude-intention link.
Simulative analysis of turbulent flow through a circular conduit with uniform wall temperature and constant heat flux boundary conditions
Authors: Belhocine Ali , Wan Omar Wan Zaidi 
Published: April 2019

PurposeThis study aims to investigate numerically a two-dimensional fully developed mean turbulent fluid flow, and heat transfer in a circular duct is numerically investigated using FORTRAN 95 code that applies the finite difference method to solve the thermal problem for the two thermal boundary conditions, constant surface temperature, constant heat and steady, axisymmetric flow. Several important results have been drawn and discussed from thermal analysis. Finally, the numerical results of the model developed in the document have been validated in good accuracy by comparing them with some correlation results available in the specialized literature.Design/methodology/approachThe methodology of solving the thermal problem is based on the equation of energy for a fluid of constant properties while taking into consideration the hypothesis of the axisymmetric and fully developed pipe flow in steady state. The global equation and the initial and boundary conditions acting on the problem have been configured here in dimensionless form to predict the turbulent behavior of the fluid inside the tube. Thus, using Thomas' algorithm, a program in FORTRAN version 95 was developed to numerically solve the discretized form of the system of equations describing the problem.FindingsThe profiles of the solutions are provided from which the authors infer that the numerical and literature correlation agreed very well. Another result that they obtained from this study is the number of Nusselt in the thermal entrance region to which a parametric study based on Reynolds and Peclet numbers, and the longitudinal coordinate, was carried out and discussed well for the impact of the scientific contribution.Originality/valueThe novelty of the work is the application of the finite difference method programed on the FORTRAN code, as a sequential numerical method of an ODEs system, to determine the number of Nusselt in both uniform wall temperature and wall heat flux uniform.