bluuebunny/crossref_metadata_2025_split huggingface dataset:
Galaxy morphologies revealed with Subaru HSC and super-resolution techniques. II. Environmental dependence of galaxy mergers at z ∼ 2–5
Authors: Shibuya Takatoshi , Ito Yohito , Asai Kenta , Kirihara Takanobu , Fujimoto Seiji , Toba Yoshiki , Miura Noriaki , Umayahara Takuya , Iwadate Kenji , Ali Sadman S , Kodama Tadayuki 
Published: November 2024

Abstract
We super-resolve the seeing-limited Subaru Hyper Suprime-Cam (HSC) images for 32187 galaxies at $z\sim 2$–5 using three techniques, namely, the classical Richardson–Lucy (RL) point spread function (PSF) deconvolution, sparse modeling, and generative adversarial networks, to investigate the environmental dependence of galaxy mergers. These three techniques generate overall similar high spatial resolution images but with some slight differences in galaxy structures; for example, more residual noises are seen in the classical RL PSF deconvolution. To alleviate the disadvantages of each technique, we create combined images by averaging over the three types of super-resolution images, resulting in galaxy substructures resembling those seen in the Hubble Space Telescope images. Using the combined super-resolution images, we measure the relative galaxy major merger fraction corrected for the chance projection effect, $f_{\rm merger}^{\rm rel,col}$, for galaxies in the $\sim$300 deg$^2$ area data of the HSC Strategic Survey Program and the CFHT Large Area U-band Survey. Our $f_{\rm merger}^{\rm rel,col}$ measurements at $z\sim 3$ validate previous findings showing that $f_{\rm merger}^{\rm rel,col}$ is higher in regions with a higher galaxy overdensity $\delta$ at $z\sim 2$–3. Thanks to the large galaxy sample, we identify a nearly linear increase in $f_{\rm merger}^{\rm rel,col}$ with increasing $\delta$ at $z\sim 4$–5, providing the highest-z observational evidence that galaxy mergers are related to $\delta$. In addition to our $f_{\rm merger}^{\rm rel,col}$ measurements, we find that the galaxy merger fractions in the literature also broadly align with the linear $f_{\rm merger}^{\rm rel,col}$–$\delta$ relation across a wide redshift range of $z\sim 2$–5. This alignment suggests that the linear $f_{\rm merger}^{\rm rel,col}$–$\delta$ relation can serve as a valuable tool for quantitatively estimating the contributions of galaxy mergers to various environmental dependences. This super-resolution analysis can be readily applied to datasets from wide field-of-view space telescopes such as Euclid and Roman.
A seed-specific DNA-binding with One Finger transcription factor, RPBF, positively regulates galactinol synthase to maintain seed vigour in rice
Authors: Sonowal Kaberi , Gandass Nishu , Kamble Nitin Uttam , Mehta Kritika , Pandey Ajay Kumar , Salvi Prafull 
Published: November 2024

Abstract
Seed vigour and longevity are intricate yet indispensable physiological traits for agricultural crops, as they play a crucial role in facilitating the successful emergence of seedlings and exert a substantial influence on crop productivity. Transcriptional regulation plays an important role in seed development, maturation, and desiccation tolerance, which are important attributes for seed vigour and longevity. Here, we have investigated the regulatory role of the seed-specific DNA-binding with One Finger (DOF) transcription factor and the rice prolamin box binding factor (RPBF) in seed vigour. RPBF modulates the transcription of galactinol synthase (GolS) and improves seed vigour. The promoter region of GolS-encoding genes from different species was enriched with DOF-binding sites, and the expression levels of both RPBF; OsGolS were found to enhance during seed development. Furthermore, direct interaction of RPBF with the OsGolS promoter has been demonstrated through multiple approaches: yeast one-hybrid assays, in planta promoter–GUS assays, dual luciferase assay, and in silico molecular docking. To assess functionality, Agrobacterium-mediated genetic transformation of rice was performed to generate the RNAi lines with reduced RPBF expression. In these RNAi lines, a reduction in both galactinol and raffinose content was observed. Since galactinol and raffinose are known contributors to seed vigour, the T2-transgenic lines were assessed for vigour and viability. For this, RNAi seeds were subjected to accelerated ageing by exposing them to high relative humidity and temperature, followed by scoring the germination and viability potential. Tetrazolium and seed germination assay revealed that the RNAi seeds were more sensitive to ageing compared to their wild-type and vector control counterparts. Collectively, this is the first report demonstrating that the DOF transcription factor RPBF controls the seed vigour through transcriptional regulation of GolS.
Jasmonate induces translation of the Arabidopsis transfer RNA-binding protein YUELAO1, which activates MYC2 in jasmonate signaling
Authors: Wang Jiahui , Li Yuanyuan , Hu Yanru , Zhu Sirui 
Published: November 2024

Abstract
Jasmonate is ubiquitous in the plant kingdom and regulates multiple physiological processes. Although jasmonate signaling has been thoroughly investigated in Arabidopsis thaliana, most studies have focused on the transcriptional mechanisms underlying various jasmonate responses. It remains unclear whether (and how) translation-related pathways help improve transcription efficiency to modulate jasmonate signaling, which may enable plants to respond to stressful conditions effectively. Here, we demonstrate that jasmonate induces translation of the transfer RNA (tRNA)-binding protein YUELAO 1 (YL1) via a specific region in its 3′ untranslated region (3′ UTR). YL1 and its homolog YL2 redundantly stimulate jasmonate responses such as anthocyanin accumulation and root growth inhibition, with the YL1 3′ UTR being critical for YL1-promoted jasmonate responses. Once translated, YL1 acts as an activator of the MYC2 transcription factor through direct interaction, and disrupting YL1 3′ UTR impairs the YL1-mediated transcriptional activation of MYC2. YL1 enhances jasmonate responses mainly in a MYC2-dependent manner. Together, these findings reveal a translational mechanism involved in jasmonate signaling and advance our understanding of the transcriptional regulation of jasmonate signaling. The YL1 3′ UTR acts as a crucial signal transducer that integrates translational and transcriptional regulation, allowing plants to respond to jasmonate in a timely fashion.
The NIH HEAL pain common data elements (CDE): a great start but a long way to the finish line
Authors: Adams Meredith C B , Hassett Afton L , Clauw Daniel J , Hurley Robert W 
Published: November 2024

Abstract

Background
The National Institutes of Health (NIH) Pain Common Data Elements (CDEs) provide a standardized framework for pain research, but their implementation and interpretation present challenges.

Objectives
To review the NIH CDE Program’s selected pain domains, provide best practices for implementing required questions, and offer a checklist for appropriate CDE use in clinical trials and secondary data analysis. This work analyzed the 10 core pain research domains selected by the NIH CDE Program and discuss their limitations and considerations for use.

Results
The manuscript provides an overview of the 10 core pain research domains, including pain intensity, interference, physical function, sleep, catastrophizing, depression, anxiety, global impression of change, substance use screening, and quality of life. It offers sample scenarios for implementing required questions and presents a checklist to guide researchers in using pain CDEs effectively for clinical trials and secondary data analysis.

Conclusion
Key challenges identified include contextual variability, lack of validation across all pain conditions and populations, and potential misuse or misinterpretation of measures. This work proposes solutions such as supplementary measures, context-specific guidance, comprehensive training programs, and ongoing refinement of the CDE framework. While NIH Pain CDEs are valuable tools for standardizing pain assessment in research, addressing challenges in their implementation and interpretation is crucial for improving the consistency, validity, and interpretability of pain research data, ultimately advancing the field and enhancing patient care.
Deep learning assessment of disproportionately enlarged subarachnoid-space hydrocephalus in Hakim’s disease or idiopathic normal pressure hydrocephalus
Authors: Yamada Shigeki , Ito Hirotaka , Iseki Chifumi , Kondo Toshiyuki , Yamanaka Tomoyasu , Tanikawa Motoki , Otani Tomohiro , Ii Satoshi , Ohta Yasuyuki , Watanabe Yoshiyuki , Wada Shigeo , Oshima Marie , Mase Mitsuhito 
Published: November 2024

Abstract

Background
Disproportionately enlarged subarachnoid-space hydrocephalus (DESH) is a key feature of Hakim’s disease (synonymous with idiopathic normal pressure hydrocephalus; iNPH). However, it previously had been only subjectively evaluated.

Purpose
This study aims to evaluate the usefulness of MRI indices, derived from deep learning segmentation of cerebrospinal fluid (CSF) spaces, for DESH detection and to establish their optimal thresholds.

Materials and Methods
This study retrospectively enrolled a total of 1009 participants, including 77 patients diagnosed with Hakim’s disease, 380 healthy volunteers, 163 with mild cognitive impairment, 256 with Alzheimer’s disease, and 217 with other types of neurodegenerative diseases. DESH, ventriculomegaly, tightened sulci in the high convexities, and Sylvian fissure dilatation were evaluated on three-dimensional T1-weighted MRI by radiologists. The total ventricles, high-convexity part of the subarachnoid space, and Sylvian fissure and basal cistern were automatically segmented using the CSF Space Analysis application (FUJIFILM Corporation). Moreover, DESH, Venthi, and Sylhi indices were calculated based on these 3 regions. The area under the receiver-operating characteristic curves of these indices and region volumes (volume ratios) for DESH detection were calculated.

Results
Of the 1009 participants, 101 (10%) presented with DESH. The DESH, Venthi, and Sylhi indices performed well with 95.0%-96.0% sensitivity and 91.5%-96.8% specificity at optimal thresholds. All patients with Hakim’s disease were diagnosed with DESH, despite variations in severity. In patients with Hakim’s disease, with or without Alzheimer’s disease, the DESH index and total ventricular volume were significantly higher compared to patients with Alzheimer’s disease, although the total intracranial cerebrospinal fluid volume was significantly lower.

Conclusion
DESH, Venthi, and Sylhi indices, and the volumes and volume ratios of the ventricle and high-convexity part of the subarachnoid space computed using deep learning were useful for the DESH detection that may help to improve the diagnosis of Hakim’s disease (ie, iNPH).
Telitacicept add-on therapy in refractory idiopathic inflammatory myopathy: insights from a pilot study
Authors: Gao Huajie , Lin Jing , Yang Mengge , Gui Mengcui , Ji Suqiong , Bu Bitao , Li Yue 
Published: November 2024

Abstract

Objectives
This study aimed to evaluate the therapeutic efficacy of telitacicept based on the conventional treatment in adults with idiopathic inflammatory myopathy (IIM), focusing on its impact on clinical manifestations.

Methods
IIM patients who had been treated with telitacicept for at least 3 months based on the conventional treatment from January 2023 to January 2024 were included in this study. The clinical response to telitacicept was determined based on the ACR/EULAR criteria for minimal, moderate and major improvement in the Total Improvement Score. Disease activity was monitored using core set measures, while myositis damage was assessed with established assessment tools. The Manual Muscle Test for eight muscle groups (MMT-8) was used to assess the muscle performance.

Results
A total of 11 patients administered with telitacicept (160 mg per week) were included in this study. Post-treatment assessments revealed improvements in all patients according to ACR/EULAR criteria. Notably, there was a significant reduction in the prednisone dosage from baseline to last visit [27.05 (12.47) to 12.05 (7.32) mg; P &lt; 0.005]. An enhancement was observed in the MMT-8 score [which improved from 109.18 (14.18) to 137.64 (15.28); P &lt; 0.005], and there was a reduction in creatine kinase level [from 2670.27 (2675.00) to 561.09 (754.09) U/l; P &lt; 0.05].

Conclusion
Telitacicept demonstrated effectiveness in treating refractory inflammatory myopathy, contributing to a significant reduction in steroid dosage among the patients. These findings highlight the potential of telitacicept as a valuable therapeutic option in the management of IIM.
Impact of structural severity on outcomes in knee osteoarthritis: an analysis of data from phase 2 and phase 3 lorecivivint clinical trials
Authors: Tambiah Jeyanesh , Kennedy Sarah , Swearingen Christopher , McAlindon Timothy , Yazici Yusuf 
Published: November 2024

Abstract

Objective
Developing knee osteoarthritis (OA) treatments is challenging due to assessing pain and joint structure outcomes within a highly heterogeneous disease. Lorecivivint (LOR), an intra-articular CLK/DYRK inhibitor, modulates Wnt and inflammatory pathways. This review analysis of LOR 0.07 mg trial data aims to describe the potential impact of baseline joint structure on OA pain response.

Methods
Two Phase 2 and two Phase 3 trials enrolled knee OA patients with Kellgren-Lawrence (KL) Grades 2–3 and Pain Numeric Rating Scale [NRS (0–10)] ≥ 4 to ≤8 in their target knee. Cumulative frequency distribution plots by KL grade summarized the percentages of patients with medial joint space width (medial JSW) &lt; 3 mm. Osteoarthritis Research Society International Joint Space Narrowing grades and treatment responses in trials capturing Pain NRS were similarly summarized. Pain outcome changes were estimated using baseline adjusted ANCOVA.

Results
Compared with phase 2 trials, the phase 3 trials had an increased proportion of patients with baseline medial JSW &lt;3 mm. LOR demonstrated beneficial treatment effects vs placebo in KL 2 subgroups, which were found to have higher proportions of baseline medial JSW &gt;3 mm, apart from one Phase 3 trial with advanced structural knee OA.

Conclusion
Baseline medial JSWs were heterogeneous across trials despite KL inclusion criteria. LOR demonstrated greater symptomatic improvements in patients with less structurally advanced disease, indicative of an association between OA structural damage and pain. Early treatment interventions may improve outcomes and provide insight for future OA trial inclusion criteria development.

Trial registration
OA-02, NCT02536833; OA-04, NCT03122860; OA-10, NCT04385303; OA-11, NCT03928184.
The lasting legacy of Charles Fisher (1908–1988), pioneering sleep laboratory scientist and sleep medicine psychiatrist
Authors: Schenck Carlos H , Provini Federica , Eiser Alan S 
Published: November 2024

Abstract
Charles Fisher is a pioneering historical figure in sleep laboratory research and sleep medicine who distinguished himself in nine areas: (1) he first documented nocturnal sleep-onset rapid eye movement (REM) sleep periods in narcoleptic patients; (2) he published the first case of polysomnography (PSG) documented acute REM sleep behavior disorder (RBD) that was triggered by sudden withdrawal from a monoamine oxidase inhibitor in 1978, 8 years before the formal identification of RBD; (3) he worked with Roffwarg and Dement on the early delineation of the ontogeny of the human sleep cycle; (4) he first demonstrated that benzodiazepine (diazepam) therapy was effective in controlling night terrors together with suppression of stage 4 non-rapid eye movement (NREM) sleep, and he was also an early investigator of night terrors as phenomena emerging from stage 4 NREM sleep, without dreaming, as had been traditionally assumed; (5) he collaborated with another pioneering sleep medicine physician, William C. Dement on studies focused on REM sleep deprivation and dreaming at Fisher’s Mt. Sinai Hospital sleep laboratory in New York City; (6) he published the first PSG-documented case of sleep-related (psychogenic) dissociative disorder in 1976; (7) he first documented that typical nightmares (“anxiety dreams”) occurred during REM sleep; (8) he conducted some of the earliest research, beginning in 1965, that documented cycles of nocturnal penile tumescence emerging in conjunction with REM sleep cycles; and (9) he conducted similar early studies of female sexual arousal during sleep that occurred predominantly in REM sleep.
HSP70 promotes amino acid‐dependent mTORC1 signaling by mediating CHIP‐induced NPRL2 ubiquitination and degradation
Authors: Gao Jianfang , Lin Mingjun , Qing Jina , Li Hongxia , Zeng Xiao , Yuan Wuzhou , Li Tingting , He Shanping 
Published: November 2024

AbstractMechanistic target of rapamycin complex 1 (mTORC1) is a master regulator of cell growth and its dysregulation leads to a variety of human diseases. Although NPRL2, an essential component of the GATOR1 complex, is reported to effectively suppress amino acid‐induced mTORC1 activation, the regulation of NPRL2 protein stability is unclear. In this study, we show that chaperon‐associated ubiquitin ligase CHIP interacts with NPRL2 and promotes its polyubiquitination and proteasomal degradation. Moreover, HSP70 mediates CHIP‐induced ubiquitination and degradation of NPRL2. Consistently, overexpression of HSP70 enhances whereas HSP70 depletion inhibits amino acid‐induced mTORC1 activation. Accordingly, knockdown of HSP70 promotes basal autophagic flux, and inhibits cell growth and proliferation. Taken together, these results demonstrated that HSP70 is a novel activator of mTORC1 through mediating CHIP‐induced ubiquitination and degradation of NPRL2.
Neurovascular dysregulation in systemic sclerosis: novel insights into pathophysiology, diagnosis, and treatment utilizing invasive cardiopulmonary exercise testing
Authors: Tarras Elizabeth , Joseph Phillip 
Published: November 2024

Purpose of review
Pathologic abnormalities in skeletal muscle and the systemic vasculature are common in patients with systemic sclerosis (SSc). These abnormalities may lead to impaired systemic peripheral oxygen extraction (EO2), known as neurovascular dysregulation, which may be because of abnormal blood flow distribution in the vasculature, microvascular shunting, and/or skeletal muscle mitochondrial dysfunction. Findings from invasive cardiopulmonary exercising testing (iCPET) provide important insights and enable diagnosis and treatment of this SSc disease manifestation.

Recent findings
Recent findings from noninvasive cardiopulmonary exercise testing (niCPET) support the existence of neurovascular dysregulation in patients with SSc. Invasive cardiopulmonary exercise testing (iCPET) has pointed to reduced systemic vascular distensibility as a possible mechanism for neurovascular dysregulation in patients with connective tissue diseases, including SSc.

Summary
Neurovascular dysregulation is likely an underappreciated cause of exercise impairment and dyspnea in patients with SSc in the presence or absence of underlying cardiopulmonary disease. It is posited to be related to microcirculatory and muscle dysfunction. Further studies are needed to clarify the pathophysiology of neurovascular dysregulation in SSc and to identify novel treatment targets and additional therapies.
Evaluating N-acetylcysteine as a Protective Agent Against Chemotherapy-induced Neuropathy in Breast Cancer
Authors: Hassanzadeh Elyas , Sedighi Pashaki Abdolazim , Akbari Hamed Ehsan , Mehrpooya Maryam , Mohammadian Kamal , Bayani Reyhaneh , Sheikhi Kamran , Ranjbar Hossein , Abbasi Mohammad 
Published: November 2024

Objectives:
Chemotherapy-induced peripheral neuropathy (CIPN) is a significant clinical issue that affects patients’ quality of life and can limit the dosing of chemotherapeutic agents. N-acetylcysteine (NAC) has been proposed as a potential chemoprotective agent against CIPN due to its antioxidant properties. This study aimed to investigate the efficacy of oral NAC in preventing and controlling taxane-induced neuropathy in patients with breast cancer.

Methods:
This randomized, triple-blind, placebo-controlled trial included 80 breast cancer patients undergoing taxane-based chemotherapy. Participants were divided into 2 groups: an intervention group receiving 1200 mg of oral NAC in divided doses per day and a placebo group. Patients were evaluated for neuropathy grade and functional status at 1 and 12 weeks postintervention.

Results:
Our analysis revealed no significant difference in the incidence and severity of neuropathy between the intervention and placebo groups at 1 (P=0.328) and 12 weeks (P=0.569) postchemotherapy. Baseline characteristics such as age, number of treatment cycles, and disease stage were similar between groups, indicating a homogeneous population.

Conclusions:
Oral NAC at a dose of 1200 mg per day did not significantly reduce the incidence or severity of taxane-induced neuropathy. These findings suggest that the oral bioavailability of NAC may be insufficient to exert a protective effect and that future studies should consider alternative dosing strategies or routes of administration. The need for further research to optimize NAC’s chemoprotective role in CIPN remains evident.
Risk factors for screw loosening in patients with lumbar degenerative spondylolisthesis treated with Dynesys dynamic stabilization
Authors: Huang Yen-Chun , Lin Hsi-Hsien , Wang Shih-Tien , Chou Po-Hsin , Liu Chien-Lin , Yao Yu-Cheng 
Published: November 2024

Background:
Posterior decompression with Dynesys dynamic stabilization (DDS) is an effective treatment option for degenerative spondylolisthesis. However, postoperative pedicle screw loosening (PSL) often results in poor outcomes. Determining possible risk factors may aid in making informed decisions. This study aimed to evaluate the incidence and risk factors for PSL in patients who underwent elective DDS.

Methods:
We conducted a retrospective analysis of 94 patients with L4-L5 grade I degenerative spondylolisthesis who underwent DSS at a single academic institution between May 2008 and February 2015. Screw loosening was defined as the presence of a halo or double-halo sign on plain radiography.

Results:
The rate of screw loosening was 4.8% among screws and 11.7% among patients. Multivariable analysis revealed that age >65 years, smoking, segmental range of motion (ROM) >13°, and L1 vertebral body Hounsfield unit <110 were risk factors for PSL after DDS. Five reoperations were performed; however, no instances of screw breakage were observed.

Conclusion:
Our study identified risk factors for PSL after DDS, including older age, smoking, larger preoperative segmental ROM, and lower L1 vertebral body HU, which could assist in preoperative decision-making and surgical planning.