bluuebunny/crossref_metadata_2025_split huggingface dataset:
Textbook Outcome
Authors: van Roessel Stijn , Mackay Tara M. , van Dieren Susan , van der Schelling George P. , Nieuwenhuijs Vincent B. , Bosscha Koop , van der Harst Edwin , van Dam Ronald M. , Liem Mike S. L. , Festen Sebastiaan , Stommel Martijn W. J. , Roos Daphne , Wit Fennie , Molenaar I. Quintus , de Meijer Vincent E. , Kazemier Geert , de Hingh Ignace H. J. T. , van Santvoort Hjalmar C. , Bonsing Bert A. , Busch Olivier R. , Groot Koerkamp Bas , Besselink Marc G. 
Published: July 2019

Background:
Textbook outcome (TO) is a multidimensional measure for quality assurance, reflecting the “ideal” surgical outcome.

Methods:
Post-hoc analysis of patients who underwent pancreatoduodenectomy (PD) or distal pancreatectomy (DP) for all indications between 2014 and 2017, queried from the nationwide prospective Dutch Pancreatic Cancer Audit. An international survey was conducted among 24 experts from 10 countries to reach consensus on the requirements for TO in pancreatic surgery. Univariable and multivariable logistic regression was performed to identify TO predictors. Between-hospital variation in TO rates was compared using observed-versus-expected rates.

Results:
Based on the survey (92% response rate), TO was defined by the absence of postoperative pancreatic fistula, bile leak, postpancreatectomy hemorrhage (all ISGPS grade B/C), severe complications (Clavien–Dindo ≥III), readmission, and in-hospital mortality. Overall, 3341 patients were included (2633 (79%) PD and 708 (21%) DP) of whom 60.3% achieved TO; 58.3% for PD and 67.4% for DP. On multivariable analysis, ASA class 3 predicted a worse TO rate after PD (ASA 3 OR 0.59 [0.44–0.80]), whereas a dilated pancreatic duct (>3 mm) and pancreatic ductal adenocarcinoma (PDAC) were associated with a better TO rate (OR 2.22 [2.05–3.57] and OR 1.36 [1.14–1.63], respectively). For DP, female sex and the absence of neoadjuvant therapy predicted better TO rates (OR 1.38 [1.01–1.90] and OR 2.53 [1.20–5.31], respectively). When comparing institutions, the observed-versus-expected rate for achieving TO varied from 0.71 to 1.46 per hospital after casemix-adjustment.

Conclusions:
TO is a novel quality measure in pancreatic surgery. TO varies considerably between pancreatic centers, demonstrating the potential benefit of quality assurance programs.
HLA Alloimmunization Following Ventricular Assist Device Support Across the Age Spectrum
Authors: Halpin Anne M. , Nahirniak Susan , Campbell Patricia M. , Urschel Simon , Kim Daniel H. , West Lori J. , Pidorochynski Tara , Buchholz Holger , Conway Jennifer 
Published: July 2019

Background.
Ventricular assist device (VAD) therapy has become an important tool for end-stage heart failure. VAD therapy has increased survival but is associated with complications including the development of human leukocyte antigen (HLA) antibodies. We sought to determine the incidence of HLA antibody development post-VAD insertion, across the age spectrum, in patients receiving leukocyte-reduced blood products, with standardized HLA antibody detection methods and to investigate factors associated with antibody development.

Methods.
This was a retrospective analysis of all patients who underwent durable VAD placement between 2005 and 2014. Inclusion criteria included availability of pre- and post-VAD HLA antibody results. Associations between HLA antibody development in the first-year postimplant and patient factors were explored.

Results.
Thirty-nine adult and 25 pediatric patients made up the study cohort. Following implant, 31% and 8% of patients developed new class I and class II antibodies. The proportion of newly sensitized patients was similar in adult and pediatric patients. The class I HLA panel reactive antibody only significantly increased in adults. Pre-VAD sensitization, age, sex (pediatrics), and transfusion were not associated with the development of HLA antibodies.

Conclusions.
In a cohort of VAD patients receiving leukocyte-reduced blood products and standardized HLA antibody testing, roughly one-third developed new class I antibodies in the first-year postimplant. Adults showed significantly increased class I panel reactive antibody following VAD support. No patient-related factors were associated with HLA antibody development. Larger prospective studies are required to validate these findings and determine the clinical impact of these antibodies following VAD insertion.
Organ Procurement Organization Run Department of Motor Vehicle Registration and Drivers Licensing Offices Leads to Increased Organ Donor First Person Authorization Registrations
Authors: Dageforde Leigh Anne , Muren William , Chang Su-Hsin , Vachharajani Neeta , Brockmeier Diane , Yu Jennifer , Anderson Blaire , Shenoy Surendra , Lin Yiing , Khan Adeel , Wellen Jason , Chapman William , Doyle Majella 
Published: July 2019

Background.
More people who have personally consented to organ donation via first person authorization (FPA) registration before death become organ donors than those not personally consenting. The majority of registrations occur at state-specific department of motor vehicle (DMV) and licensing offices, where people register their vehicles and obtain driver's licenses.

Methods.
One organ procurement organization (OPO) ran 3 DMV offices and implemented an intervention: a donor-centric approach, including employee education, office decoration with donation materials, and customer experience improvements. Data about registry enrollment was collected before and during the 4-year OPO licensing office contract. A linear mixed model and interrupted time series analyses were performed to evaluate whether the intervention improved rates of registration.

Results.
Preintervention registry enrollment rates per month were 10%–50%. Having the offices run by an OPO was associated with more enrollments independent of the increasing trend of enrollment (P < 0.001). Also, the DMV office with the lowest preimplementation registration rates had an immediate increase in enrollments after the intervention leading to higher registration rates (P < 0.001).

Conclusions.
A donor-centric OPO-managed DMV experience increases FPA registration, especially at offices with low initial registration rates. However, even at the office with the highest percentage of FPA registrations, rates were only 65% at intervention conclusion. The transplant community should consider other opportunities for FPA registration.
Hierarchical Compression Reveals Sub-Second to Day-Long Structure in Larval Zebrafish Behaviour
Authors: Ghosh Marcus , Rihel Jason 
Published: July 2019

AbstractAnimal behaviour is dynamic, evolving over multiple timescales from milliseconds to days and even across a lifetime. To understand the mechanisms governing these dynamics, it is necessary to capture multi-timescale structure from behavioural data. Here, we develop computational tools and study the behaviour of hundreds of larval zebrafish tracked continuously across multiple 24-hour day/night cycles. We extracted millions of movements and pauses, termed bouts, and used unsupervised learning to reduce each larva’s behaviour to an alternating sequence of active and inactive bout types, termed modules. Through hierarchical compression, we identified recurrent behavioural patterns, termed motifs. Module and motif usage varied across the day/night cycle, revealing structure at sub-second to day-long timescales. We further demonstrate that module and motif analysis can uncover novel pharmacological and genetic mutant phenotypes. Overall, our work reveals the organisation of larval zebrafish behaviour at multiple timescales and provides tools to identify structure from large-scale behavioural datasets.
Systems-level immunomonitoring using self-sampled capillary blood
Authors: Josyula Vijay Sai , Lakshmikanth Tadepally , Mikes Jaromir , Chen Yang , Brodin Petter 
Published: July 2019

AbstractComprehensive profiling of the human immune system in patients with cancer, autoimmune disease and during infections are providing valuable information that help us understand disease states and discriminate productive from inefficient immune responses and identify possible targets for immune modulation. Recent technical advances now allow for all immune cell populations and hundreds of plasma proteins to be detected using small volume blood samples. To democratize such systems-immunological analyses, further simplified blood sampling and preservation will be important. Here we describe that blood obtained via a nearly painless self-sampling device of 100 microliter of capillary blood that is preserved and frozen, can simplify systems-level immunomonitoring studies.
Evaluation of Bilateral Asymmetry in the Humerus of Human Skeletal Specimen
Authors: Dare Samuel S. , Masilili Godfery , Mugagga Kintu , Ekanem Peter E. 
Published: July 2019

ABSTRACTSeveral studies have established a relationship between morphological and behavioural asymmetry making investigations of bilateral bone asymmetry an attractive and important research area. The purpose of this study was to investigate bilateral asymmetry patterns of skeletal specimen from five geographical locations (Rwanda, Burundi, Congo, Kenya and Uganda) at Galloway Osteological Collection, Department of Anatomy, School of Biomedical Sciences, Makerere University College of Health Sciences at Makerere University.The angle of torsion and retroversion, mid shaft circumference, length and weight of 232 pairs of humeri were determined. A Torsiometer was used to measure the angle of torsion in degrees according to Krahl and Evans 1945, a tape was used to measure the mid shaft circumference at the level of the apex of the deltoid V and the length in cm was determined. An osteomeric board was used to measure the length of the humerus in centimeters. A weighing balance was used to measure the weight of the humerus in grams.The analysis of humeral asymmetry with respect to parameters of the human skeletal specimen at the Galloway Osteological collection Mulago revealed bilateral asymmetrical status observed in the angle of torsion, length, weight and mid-shaft circumference. Our result mostly showed lateralization to the right in all the parameters investigated except the torsion angle which is to the left.Our investigation revealed that humeral torsion is inversely proportional to weight, length and mid-shaft circumference of the humerus. This study established the existence of bilateral asymmetries in the humeri of all the geographical regions investigated with more asymmetry observed in the male compared with the female.
Novel insights into the taxonomic diversity and molecular mechanisms of bacterial Mn(III) reduction
Authors: Szeinbaum Nadia , Nunn Brook L. , Cavazos Amanda R. , Crowe Sean A. , Stewart Frank J. , DiChristina Thomas J. , Reinhard Christopher T. , Glass Jennifer B. 
Published: July 2019

SummarySoluble ligand-bound Mn(III) can support anaerobic microbial respiration in diverse aquatic environments. Thus far, Mn(III) reduction has only been associated with certain Gammaproteobacteria. Here, we characterized microbial communities enriched from Mn-replete sediments of Lake Matano, Indonesia. Our results provide the first evidence for biological reduction of soluble Mn(III) outside the Gammaproteobacteria. Metagenome assembly and binning revealed a novel betaproteobacterium, which we designate “Candidatus Dechloromonas occultata.” This organism dominated the enrichment and expressed a porin-cytochrome c complex typically associated with iron-oxidizing Betaproteobacteria and a novel cytochrome c-rich protein cluster (Occ), including an undecaheme putatively involved in extracellular electron transfer. This occ gene cluster was also detected in diverse aquatic bacteria, including uncultivated Betaproteobacteria from the deep subsurface. These observations provide new insight into the taxonomic and functional diversity of microbially-driven Mn(III) reduction in natural environments.Originality-Significance StatementRecent observations suggest that Mn(III)-ligand complexes are geochemically important in diverse aquatic environments. Thus far, microbially-driven Mn(III) reduction has only been associated with Gammaproteobacteria encoding three-component outer-membrane porin-cytochrome c conduits. Here, we demonstrate that Betaproteobacteria dominate in abundance and with respect to protein expression during biologically-mediated Mn(III) reduction in an enrichment culture from an anoxic lacustrine system. Using metaproteomics, we detect for the first time that Betaproteobacteria express a two-component porin-cytochrome c conduit, and an uncharacterized extracellular undecaheme (11-heme) c-type cytochrome. Although this is the first definitive report of an undecaheme within the Betaproteobacteria, we find evidence that they are widespread in uncultivated strains. These results widen the phylogenetic diversity of Mn(III)-reducing bacteria, and provide new insights into potential molecular mechanisms for soluble Mn(III) reduction
Bacterial fucosidase enables the production of Bombay red blood cells
Authors: Li Tiansheng , Ye Juan , Wang Lei , Zou Lin , Guo Yameng , Hou Linlin , Shen Danfeng , Cai Xiaohong , Huang Haobo , Sun Guiqin , Chen Li 
Published: July 2019

AbstractWe present a method to produce H antigen-deficient red blood cells (RBCs) for transfusion to individuals with anti-H antibodies. A fucosidase from bacteria was heterologously expressed efficiently in E. coli and has been demonstrated to completely remove H antigens on the surface of human RBCs in a facile conversion process. The approach we describe here holds promise for making H-deficient RBCs available for a rare population beyond ABO types.
Ultra High-Throughput Multiparametric Imaging Flow Cytometry: Towards Diffraction-Limited Sub-Cellular Detection
Authors: Holzner Gregor , Mateescu Bogdan , van Leeuwen Daniel , Cereghetti Gea , Dechant Reinhard , deMello Andrew , Stavrakis Stavros 
Published: July 2019

ABSTRACTFlow cytometry is widely recognized as the gold-standard technique for the analysis and enumeration of heterogeneous cellular populations and has become an indispensable tool in diagnostics,1rare-cell detection2and single-cell proteomics.3Although contemporary flow cytometers are able to analyse many thousands of cells per second, with classification based on scattering or fluorescence criteria, the vast majority require unacceptably large sample volumes, and do not allow the acquisition of spatial information. Herein, we report a sheathless, microfluidic imaging flow cytometer that incorporates stroboscopic illumination for blur-free fluorescence and brightfield detection at analytical throughputs in excess of 60,000 cells/s and 400,000 cells per second respectively. Our imaging platform is capable of multi-parametric fluorescence quantification and subcellular (co-)localization analysis of cellular structures down to 500 nm with microscopy image quality. We demonstrate the efficacy of our approach by performing challenging high-throughput localization analysis of cytoplasmic RNA granules in yeast and human cells. Results suggest significant utility of the imaging flow cytometer in the screening of rare events at the subcellular level for diagnostic applications.
Neck Musculoskeletal Model Generation through Anthropometric Scaling
Authors: Roos Paulien E. , Vasavada Anita , Zheng Liying , Zhou Xianlian 
Published: July 2019

ABSTRACTA new methodology was developed to quickly generate whole body models with detailed neck musculoskeletal architecture that are properly scaled in terms of anthropometry and muscle strength. This method was implemented in an anthropometry model generation software that allows users to interactively generate any new male or female musculoskeletal models with adjustment of anthropometric parameters (such as height, weight, neck circumference, and neck length) without the need of subject-specific motion capture or medical images. 50thpercentile male and female models were developed based on the 2012 US Army Anthropometric Survey (ANSUR II) database and optimized with a novel bilevel optimization method to have strengths comparable to experimentally measured values in the literature. Other percentile models (ranging from the 1stto 99thpercentile) were generated based on anthropometric scaling of the 50thpercentile models and compared. The resultant models are reasonably accurate in terms of both musculoskeletal geometry and neck strength, demonstrating the effectiveness of the developed methodology for interactive neck model generation with anthropometric scaling.
Exploring the Dynamics of Nonlinear Biochemical Systems using Control-Based Continuation
Authors: Gomes Brandon , de Cesare Irene , Guarino Agostino , di Bernardo Mario , Renson Ludovic , Marucci Lucia 
Published: July 2019

AbstractMathematical modelling is routinely used in Systems Biology to understand the mechanisms causing nonlinear phenomena in gene expression, such as switch-like behaviours and temporal oscillations. The reliability of model predictions and bifurcation analysis depend on modelling assumptions and specific choices of model parameters; however, the identification of models is highly challenging due to the complexity of biochemical interactions and noise in experimental data.This paper numerically investigates the use of control-based continuation (CBC) for tracking dynamical features of biochemical systems and, in particular, the bistable dynamics of a gene regulating pluripotency in embryonic stem cells.CBC is a method that exploits feedback control and path following algorithms to explore the dynamic features of a nonlinear physical system directly during experimental tests. CBC applications have so far been limited to non-living (i.e. electro-mechanical) systems. Our numerical simulations show that, in principle, CBC could also be applied to biological experiments to characterise the switch-like dynamics of genes that are important for cell decision making.
Intraspecific interactions in the annual legume Medicago minima are shaped by both genetic variation for competitive ability and reduced competition among kin
Authors: Tomiolo Sara , Thomas Claire , Jespersen Michael K. , Damgaard Christian F. , Ehlers Bodil K. 
Published: July 2019

AbstractKnowing which mechanisms drive the outcome of intraspecific interactions is highly relevant for understanding diversity maintenance. Plant species that exhibit strong genetic substructure over small spatial scales may be exposed to frequent interactions with closely related individuals. Predictions of how genetic similarity may drive the outcome of intraspecific interactions are based on two contrasting theories: the resource partitioning hypothesis and kin selection theory. The first predicts that competition will be stronger among closely related conspecific (i.e. kin) because similar genotypes have similar resource requirements. The second predicts instead that competition will be reduced among kin, in order to maximize the inclusive fitness. Although efforts have been made to reconcile these two theories as non-mutually exclusive, the outcomes of intraspecific interaction studies are frequently interpreted as the results of either one or the other. We experimentally tested the hypothesis that intraspecific interactions may be driven by both genetic variation for competitive ability and reduced competition among kin. We used an annual legume, Medicago minima, to conduct two greenhouse experiments testing changes in root behaviour, above-ground growth and biomass in response to neighbour identity. We found evidence of both genetic variation for competitive ability and reduced competition among kin in some genotypes. Reduced competitive growth towards kin was found in the most competitive genotypes, suggesting that kin avoidance and competitive ability were simultaneously affecting plant behaviour and growth. With presence of both kin competition avoidance and variation for competitive ability, the outcome of intraspecific interactions will strongly depend on the local spatial genetic substructure. This is highly relevant to predict how intraspecific competition affect diversity maintenance.
Dynamic mass transfer of textiles in rotational motions
Authors: Li Wenbin , Jiang Yunjing , Jiang Peiqing , Wang Xin 
Published: July 2019

Purpose
The mass transfer of textiles during movement is complicated as the energy consumption (EC) from skin, surface temperature of fabrics together with environment will work synergistically to determine the sensation and comfort of wearer. The purpose of this paper is to reveal the mass transfer in the human-textile-environment dynamic system.

Design/methodology/approach
With a simulated hotplate mounted on a rotational testing platform, this paper focuses on the dynamic mass transfer of a fabric so as to simulate the real-time mass transfer of clothing in movements.

Findings
It has been found that the EC and surface temperature (T) change against testing time, indicating the convex and concave shapes of the EC–t and T–t curves. The initial moisture regain of the fabric, rotational speed of the platform and the fiber materials of the fabric have shown a great effect on the dynamic mass transfer process.

Practical implications
Understanding the dynamic mass transfer of textiles will benefit the design of clothing with better comfort and will contribute to the well-being of wearers.

Originality/value
This work reveals the dynamic mass transfer of textiles in rotational movements. It contributes a new approach to studying the mass transfer of clothing in real service.
Understanding Facebook to Instagram migration: a push-pull migration model perspective
Authors: Hou Avus C.Y. , Shiau Wen-Lung 
Published: July 2019

Purpose
Emerging social networking sites (SNSs) are less advantageous than leading SNSs in attracting users. They might stand a better chance if they know what users want. The purpose of this paper is to study factors that urge Facebook users to switch to Instagram to reveal how latecomers in the SNSs circuit can win the hearts of users.

Design/methodology/approach
This study proposes an SNS switching framework that is adapted from the demographic Push-Pull (PP) migration model to investigate users’ switching intention. Structural equation modeling was applied to analyze the data collected from 260 Instagrammers who all had experience using Facebook before moving on Instagram.

Findings
Results show that socializing and system quality of the SNS negatively affect users’ switching intention, while attractiveness of the alternative, peer influence and critical mass do the opposite. Surprisingly, enjoyment is not associated with switching intention.

Research limitations/implications
SNSs switching may not mean a complete abandonment of previous SNSs. In many occasions, users simply become less active in one SNS and more active in other SNSs. The PP migration model provides a useful tool to understand the patterns as well as competing forces that influence the migration of SNS users, pushing them away or pulling them to new alternative sites. Specifically, pulling demonstrates to be a stronger influence than pushing.

Practical implications
This study suggests that SNS operators should satisfy users’ needs for socializing, maintain high system quality, provide peer influence tools and create their own attractive features, in order to retain existing customers or induce new users to switch.

Originality/value
This is one of the earlier empirical studies to investigate users’ switching intention from Facebook to Instagram with a valid sample. In addition, the present study approaches pull and push effects by multiple constructs, providing a clearer picture of what constitutes the pull and push forces.