bluuebunny/crossref_metadata_2025_split huggingface dataset:
A YoeB toxin from A. tumefaciens has metal-dependent DNA cleaving activity
Authors: McGillick Julia , Ames Jessica R. , Murphy Tamiko , Reddem Eswar , Bourne Christina R. 
Published: October 2019

ABSTRACTToxin-antitoxin (TA) systems, including YoeB-YefM, are important mediators of bacterial physiological changes. Agrobacterium tumefaciens YoeB and YefM are similar to that from E. coli, and interact as a tight heterotetramer with a KD of 653 pM. We have verified that AtYoeB can perform both ribosome-dependent and –independent RNA cleavage. We have also characterized a newly described metal-dependent and pH-sensitive DNA cleaving ability. We note that this DNA cleaving ability is observed at toxin concentrations as low as 150 nM. The dose-dependence of in vitro ribosome-independent RNA and metal-dependent DNA cleavage is equivalent, and requires a ten-fold increase in toxin concentration as opposed to in the presence of the ribosome. The toxin concentration inside bacterial cells is unknown and according to current models, should increase upon activation of YoeB through degradation of the YefM antitoxin. The discovery of general nuclease activity by AtYoeB, and perhaps other YoeB toxins, offers an opportunity to explore the plasticity of this protein fold and its potential role in the evolution of nucleases.
Sensitivity to gains during risky decision-making differentiates chronic cocaine users from stimulant-naïve controls
Authors: Kluwe-Schiavon B. , Kexel A. , Manenti G. , Cole D.M. , Baumgartner M.R. , Grassi-Oliveira R. , Tobler P.N. , Quednow B.B. 
Published: October 2019

AbstractBackgroundAlthough chronic cocaine use has been frequently associated with decision-making impairments that are supposed to contribute to the development and maintenance of cocaine addiction, it has remained unclear how risk-seeking behaviours observed in chronic cocaine users (CU) come about. Here we therefore test whether risky decision-making observed in CU is driven by alterations in individual sensitivity to the available information (gain, loss, and risk).MethodA sample of 96 participants (56 CU and 40 controls) performed the no-feedback (“cold”) version of the Columbia Card Task. Structured psychiatric interviews and a comprehensive neuropsychological test battery were additionally conducted. Current and recent substance use was objectively assessed by toxicological urine and hair analysis.ResultsCompared to controls, CU showed increased risk-seeking in unfavourable decision scenarios in which the risk was high and the returns were low, and a tendency for increased risk aversion in favourable decision scenarios. These differences arose from the fact that CU were less sensitive to gain, but similarly sensitive to loss and risk information in comparison to controls. Further analysis revealed that individual differences in sensitivity to loss and risk were related to cognitive performance and impulsivity.ConclusionThe reduced sensitivity to gain information in people with CU may contribute to their propensity for making risky decisions. While these alterations in the sensitivity to gain might be directly related to cocaine use per se, the individual psychopathological profile of CU might moderate their sensitivity to risk and loss impulsivity.
The Lipid Elongation Enzyme ELOVL2 is a molecular regulator of aging in the retina
Authors: Chen Daniel , Chao Daniel L. , Rocha Lorena , Kolar Matthew , Huu Viet Anh Nguyen , Krawczyk Michal , Dasyani Manish , Wang Tina , Jafari Maryam , Jabari Mary , Ross Kevin D. , Saghatelian Alan , Hamilton Bruce , Zhang Kang , Skowronska-Krawczyk Dorota 
Published: October 2019

ABSTRACTMethylation of the regulatory region of the Elongation Of Very Long Chain Fatty Acids-Like 2 (ELOVL2) gene, an enzyme involved in elongation of long-chain polyunsaturated fatty acids, is one of the most robust biomarkers of human age, but the critical question of whether ELOVL2 plays a functional role in molecular aging has not been resolved. Here, we report that Elovl2 regulates age-associated functional and anatomical aging in vivo, focusing on mouse retina, with direct relevance to age-related eye diseases. We show that an age-related decrease in Elovl2 expression is associated with increased DNA methylation of its promoter. Reversal of Elovl2 promoter hypermethylation in vivo through intravitreal injection of 5-Aza-2’-deoxycytidine (5-aza-dc) leads to increased Elovl2 expression and rescue of age-related decline in visual function. Mice carrying a point mutation C234W that disrupts Elovl2-specific enzymatic activity show electrophysiological characteristics of premature visual decline, as well as early appearance of autofluorescent deposits, well-established markers of aging in the mouse retina. Finally, we find deposits underneath the retinal pigment epithelium in Elovl2 mutant mice, containing components of complement system and lipid metabolism. These findings indicate that ELOVL2 activity regulates aging in mouse retina, provide a molecular link between polyunsaturated fatty acids elongation and visual functions, and suggest novel therapeutic strategies for treatment of age-related eye diseases.
Dynamical Systems Modeling of Bt Resistant Cornborer Population Dynamics
Authors: Rabajante Jomar F. , Jacildo Arian J. , Alcantara Edwin P. 
Published: October 2019

SummaryMathematical models provide insights for the design and optimization of strategies to control disease epidemics and evolution of pesticide resistance in crops. Here, we present a simple mathematical model to investigate the population dynamics of non-Bt resistant and Bt resistant cornborers in a field with refuge. In the presence of refuge, it is expected that the population of Bt resistant pests will decline due to the dilution effect (non-Bt resistant cornborers mate with Bt resistant pests). We have found that increasing the refuge size can be effective in reducing Bt resistant pests as long as there exists a relatively huge population of non-Bt resistant cornborers at the start of the simulation. This implies that refuge is useless in inhibiting the evolution of cornborers if a sufficient initial population of non-Bt resistant cornborers is absent.
Human astrocytes and microglia show augmented ingestion of synapses in Alzheimer’s disease via MFG-E8
Authors: Tzioras Makis , Daniels Michael J.D. , Davies Caitlin , Baxter Paul , King Declan , McKay Sean , Varga Balazs , Popovic Karla , Hernandez Madison , Stevenson Anna J. , Barrington Jack , Drinkwater Elizabeth , Borella Julia , Holloway Rebecca K. , Tulloch Jane , Latta Clare , Kandasamy Jothy , Sokol Drahoslav , Smith Colin , Miron Veronique E. , Karadottir Ragnhildur Thora , Hardingham Giles E. , Henstridge Christopher M. , Brennan Paul M. , McColl Barry W. , Spires-Jones Tara L. 
Published: October 2019

SummarySynapse loss correlates with cognitive decline in Alzheimer’s disease (AD). Data from mouse models suggests microglia are important for synapse degeneration, but direct human evidence for any glial involvement in synapse removal in human AD remains to be established. Here we observe astrocytes and microglia from human brains contain greater amounts of synaptic protein in AD compared to non-disease controls, and that proximity to amyloid-β plaques and theAPOE4risk gene exacerbate this effect. In culture, mouse and human astrocytes and primary mouse and human microglia phagocytose AD patient-derived synapses more than synapses from controls. Inhibiting MFG-E8 function rescued the elevated engulfment of AD synapses by astrocytes and microglia without affecting control synapse uptake. Thus, AD promotes increased synapse ingestion by human glial cells via an MFG-E8 opsonophagocytic mechanism with potential for targeted therapeutic manipulation.One-Sentence SummaryGlial cells ingest synapses in Alzheimer’s disease and antibody treatment reduces this ingestion in cultured human cells.
Observer-based reaction wheel fault reconstruction for spacecraft attitude control systems
Authors: Jia Qingxian , Li Huayi , Chen Xueqin , Zhang Yingchun 
Published: October 2019

Purpose
The purpose of this paper is to achieve fault reconstruction for reaction wheels in spacecraft attitude control systems (ACSs) subject to space disturbance torques.

Design/methodology/approach
Considering the influence of rotating reaction wheels on spacecraft attitude dynamics, a novel non-linear learning observer is suggested to robustly reconstruct the loss of reaction wheel effectiveness faults, and its stability is proven using Lyapunov’s indirect method. Further, an extension of the proposed approach to bias faults reconstruction for reaction wheels in spacecraft ACSs is performed.

Findings
The numerical example and simulation demonstrate the effectiveness of the proposed fault-reconstructing methods.

Practical implications
This paper includes implications for the development of reliability and survivability of on-orbit spacecrafts.

Originality/value
This paper proposes a novel non-linear learning observer-based reaction wheels fault reconstruction for spacecraft ACSs.
Examining the impact of case-based learning on student engagement, learning motivation and learning performance among university students
Authors: Raza Syed Ali , Qazi Wasim , Umer Bushra 
Published: October 2019

Purpose
The purpose of this paper is to examine the impact of case study-based learning on student’s engagement, learning motivation and learning performance among university students of Pakistan.

Design/methodology/approach
The research model is analyzed with structural equation modeling. The analysis is done with the application of the measurement model and the structural model.

Findings
The findings revealed that case study-based learning enhances student engagement, and a significant and positive relationship between case-based learning and all four aspects of engagement, i.e. behavioral, emotional, cognitive and agentic engagement, was observed statistically. According to the findings, case-based learning leads toward an understanding of concepts learned in class and the development of skills among students and results in the enhancement of learning motivation. Furthermore, it is suggested by the results that the impact of student engagement on learning performance varies with respect to its different aspects. As among all the four aspects, only agentic engagement was found to be statistically significant in establishing the link with the learning performance of the students.

Research limitations/implications
The mixed findings pertaining to the impact of student engagement’s aspects on learning performance of the students pave the way for future research. The future researchers should incorporate individual traits and the specific context to analyze the relationships between the proposed constructs because students vary in their responses to learning activities.

Practical implications
The present study holds significance for the field of educational research and emphasizes the importance of incorporating case-based teaching in the higher education curriculum in order to ensure the implementation of effective learning strategies. Also, this research will be beneficial for future researchers in understanding the phenomenon of student learning and engagement.

Originality/value
In view of the limited academic literature in the Asian context, the present study extends the findings and examined the impact of case-based learning on student’s engagement, student’s learning motivation and the role of such engagement in enhancing learning performance of university students.
Learnings from implementing telemonitoring – the LITE study
Authors: Chow Josephine S.F. , Gonzalez-Arce Veronica Eugenia , Knight Andrew , Maurya Nutan , Kohler Friedbert 
Published: October 2019

Purpose
A recent study on the implementation of telemonitoring has provided an opportunity to explore how telemonitoring could be further developed to enhance better patient, carer and clinician experiences for patients living with chronic disease. The purpose of this paper is to identify the challenges and lessons of the telemonitoring implementation and to identify strategies to inform future implementation.

Design/methodology/approach
A qualitative study was conducted with a purposive sample using semi-structured interviews to explore their views about the issues identified in the research aims. Interview themes were informed by current literature and the findings from local studies. Interviews were recorded, transcribed verbatim and analyzed thematically.

Findings
A total of 16 participants were interviewed. Overall the recruitment of 56 percent were from identified potential participants. Five core themes were identified, linking different aspects of the telemonitoring implementation: impact of telemonitoring; implementation and management lessons; program risks; program main changes from beginning; and future strategies and recommendations.

Originality/value
This study will inform the wider roll out of telemonitoring across the region with the goal of ensuring future interventions achieve better clinical outcomes, patient, carer experience and deliver better value.
Patient and public involvement in integrated psychosocial care
Authors: Vassiliadou Io , Tolani Esther , Ip Lindsay , Smith Abigail , Papachristou Nadal Iliatha 
Published: October 2019

Purpose
Recent models of care incorporate service user involvement within the development and sustainability of a quality improvement project. The purpose of this paper is to demonstrate the significance of working with patients and members of the public for the integration of psychosocial care into long-term condition (LTC) management.

Design/methodology/approach
Research shows that mental health difficulties are more prevalent in people with LTC. The three Dimensions for Long-term Conditions (3DLC) is a patient-centred multidisciplinary service which integrates psychological and social care into the usual physical care. Thematic analysis was conducted on the discussions of the two patient and public involvement workshops that were facilitated by the service. The workshops included healthcare professionals, patients with LTC and their carers.

Findings
Several themes and subthemes emerged which highlighted the importance of discussing and treating mental health in a physical health setting, the challenges that both the patients and healthcare professionals encounter and the ways in which an integrated care service may address these barriers. The findings show that there was an emphasis on patient-centeredness, accessibility of services and the need for better communication.

Practical implications
People with LTC can be empowered to better self-manage their condition, whilst having access to all types of care, physical, social and psychological. By involving service users in the implementation process of the 3DLC service, the components of an effective integrated service are delineated.

Originality/value
The service users have identified barriers and facilitators of integrating a biopsychosocial model in care pathways. This has helped the 3DLC team to further develop the model to ensure improvements in condition-specific outcomes, quality of life and healthcare utilisation.
Integrated care: a definition from the perspective of the four quality paradigms
Authors: Van Kemenade Everard , van der Vlegel-Brouwer Wilma 
Published: October 2019

Purpose
The purpose of this paper is to support the ongoing dialogue and shed light on the different views on integrated care. An overarching definition of integrated care is proposed combining the ways of thinking of the four quality paradigms the authors identify. The idea of epistemic fluency offers a way-out of ongoing discussions about “what integration is”.

Design/methodology/approach
Four paradigms of quality are presented and applied to healthcare. Epistemic fluency is proposed as the capacity to understand, switch between and combine different kinds of knowledge. The authors compare previously developed definitions of integrated care to the various combinations of paradigms.

Findings
All four paradigms of care quality are present in healthcare and in the most used definitions of integrated care. The Reflective Paradigm and the Emergence Paradigm receive least attention. Some definitions combine more than one paradigm. An overarching definition of integrated care is proposed.

Research limitations/implications
In this paper, only the most prominent definitions of integration have been considered.

Practical implications
Integration research and practice requires a widely accepted definition of integrated care, embracing all four paradigms of care quality. Our suggestion provides a common foundation that may prevent misunderstanding.

Originality/value
The use of quality management paradigms to frame the debate on defining integrated care is new and leads to new insights for teaching, research and practice.
Linking knowledge sharing to innovative behavior: the moderating role of collectivism
Authors: Pian Qian Ya , Jin Hui , Li Hui 
Published: October 2019

PurposeThe purpose of this study is to examine the relationship between behavior-oriented knowledge sharing and innovative behavior and the moderating effects of collectivism on the aforementioned relationship. It also assesses the impact of epistemic motivation and pro-social motivation on behavior-oriented knowledge sharing.Design/methodology/approachFocusing on Chinese context, the study conducts a questionnaire survey to test the research model. Linear regression analysis is used to examine the main effects of the independent variables, and the multi-level linear regression model is used to evaluate the moderating effects of the controlled variables.FindingsThe findings reveal that epistemic motivation stimulates individual-oriented knowledge sharing and pro-social motivation stimulates organization-oriented knowledge sharing. Organization-oriented knowledge sharing impacts more than individual-oriented knowledge sharing on innovative behavior. Moreover, collectivism is shown to positively moderate the relationship between behavior-oriented knowledge sharing and innovative behavior.Practical implicationsThe study provides evidence that motivation should be significantly considered when sharing knowledge. Managers should prioritize the promotion of employees’ epistemic and pro-social motivation. The study also suggests that encouraging collectivism should be an important objective, as it moderates the relationship between knowledge sharing and innovative behavior positively.Originality/valueThe study emphasized how individual-oriented and organization-oriented knowledge sharing impacts innovative behavior differently and how collectivism moderates that relationship. It also illustrates how epistemic and pro-social motivation affects behavior-oriented knowledge sharing. The study contributes to a stream of research that links knowledge sharing and innovative behavior.
Deleuzoguattarian place marketing: becoming, between, beneath and beyond
Authors: Coffin Jack 
Published: October 2019

Purpose
The purpose of this paper is to demonstrate how the work of Deleuze and Guattari can help place marketers to think differently about places and place brands.

Design/methodology/approach
This is a conceptual paper that draws together a range of resources to develop a Deleuzoguattarian approach to place marketing.

Findings
Deleuzoguattarian thinking helps place marketers to reconceptualise places as “becomings”, which in turn encourages them to look between, beneath and beyond their usual foci. The Deleuzoguattarian spirit of critical-creativity is also noted, encouraging readers to develop the ideas presented here in new directions.

Research limitations/implications
This paper expands the epistemological imagination of place marketing scholars to consider the places between their place brands, the subconscious influences beneath the surface of salience and phenomena beyond the anthroposcale of everyday experience. This enriches existing conceptualisations and extends place marketing with several new areas of enquiry that can be empirically elaborated through future research.

Practical implications
This paper helps place marketing practitioners to consider and respond to the flows of matter–energy that influence their place brands between, beneath and beyond their intentional management practices.

Social implications
This paper develops critical schools of thought within the place marketing literature, providing some suggestions about how to develop and manage more inclusive place brands. This may also have implications for activists and others seeking societal improvements.

Originality/value
This paper develops a Deleuzoguattarian approach to place marketing, stimulating new lines of inquiry and experimental practices.
Reliving the Terror: Victims and Print Culture during the Thermidorian Reaction in France, 1794–1795
Authors: FAIRFAX‐CHOLMELEY ALEX 
Published: October 2019

AbstractThis article analyses the Thermidorian Reaction in France (1794–5), a period when the country and its inhabitants were struggling to deal with the violent legacy of the Terror of the preceding eighteen months. Beginning with the immediate aftermath of the fall of Maximilien Robespierre on 9 Thermidor Year II/27 July 1794, it offers a fresh perspective on this troubled period by focusing on victims of the Terror (both the dead and the living). A lively print culture emerged in which both individual victims and affected communities sought to reframe their past experiences to their own benefit, in particular via campaigns of rehabilitation. They also engaged in acts of denunciation which provided another link back into the Terrorist past. Collectively, these printed outputs and the methods by which they were constructed provide a useful parallel to more well‐known developments on the national stage, where politicians were also grappling with the challenges left by the Terror.
An Analysis of the Published Literature on the Effects of Edible Coatings Formed by Polysaccharides and Essential Oils on Postharvest Microbial Control and Overall Quality of Fruit
Authors: de Souza Evandro L. , Lundgren Giovanna A. , de Oliveira Kataryne Á. R. , Berger Lúcia R. R. , Magnani Marciane 
Published: October 2019

AbstractConsumers have shown increased concern about the importance of adopting regular fresh fruit consumption. Because fresh fruit are highly susceptible to postharvest decay, several studies have focused on the development of alternative technologies to extend their market period. The application of polysaccharides in combination with essential oils (EOs) to formulate edible coatings has been considered an innovative strategy to reduce postharvest losses in fruit. However, available studies have used different methodological procedures related to the production and application of these coatings on fruit, which could be potential influential factors on the achievement of the desired effects in coated fruit. This review summarized the studies focusing on the application of edible coatings formed by polysaccharides and EOs to preserve fruit, in addition to examine and discuss possible factors affecting their functionalities. The approach given in this review envisages to contribute to research in edible coatings formed by polysaccharides and EOs and help to their optimized application as a postharvest treatment of fruit. Despite of the different methods selected for use in experimental assays, data of available literature demonstrate that coatings formed by polysaccharides (that is, chitosan—the only polysaccharide used as an antimicrobial, cassava starch, flaxseed gum, gum arabic, hydroxypropylmethylcellulose, locust bean gum, mesquite gum, pectin, pullulan, and sodium alginate) and different EOs (or their individual constituents) are effective to reduce postharvest losses in fruit and generally do not adversely affected their physicochemical and sensory characteristics during storage.