bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of betaine as a novel psychotherapeutic for schizophrenia
Authors: Ohnishi Tetsuo , Balan Shabeesh , Toyoshima Manabu , Maekawa Motoko , Ohba Hisako , Watanabe Akiko , Iwayama Yoshimi , Shimamoto-Mitsuyama Chie , Nozaki Yayoi , Hisano Yasuko , Esaki Kayoko , Nagaoka Atsuko , Matsumoto Junya , Hino Mizuki , Mataga Nobuko , Hayashi-Takagi Akiko , Kunii Yasuto , Kakita Akiyoshi , Yabe Hirooki , Yoshikawa Takeo 
Published: April 2019

ABSTRACTBetaine is known to act against various biological stresses and its levels were reported to be decreased in schizophrenia patients. Using Chdh (a gene for betaine synthesis)-deficient mice and betaine-supplemented inbred mice, we assessed the role of betaine in psychiatric pathophysiology, and its potential as a novel psychotherapeutic, by leveraging metabolomics, behavioral-, transcriptomics and DNA methylation analyses. The Chdh-deficient mice revealed remnants of psychiatric behaviors along with schizophrenia-related molecular perturbations. Betaine supplementation elicited genetic background-dependent improvement in cognitive performance, and suppressed methamphetamine (MAP)-induced behavioral sensitization. Furthermore, betaine rectified the altered antioxidative and proinflammatory responses induced by MAP and in vitro phencyclidine treatments. Notably, betaine levels were decreased in the postmortem brains from schizophrenia, and a coexisting elevated carbonyl stress, a form of oxidative stress, demarcated a subset of schizophrenia with “betaine deficit-oxidative stress pathology”. We revealed the decrease of betaine levels in glyoxylase 1 (GLO1)-defícient hiPSCs, which shows elevated carbonyl stress, and the efficacy of betaine in alleviating it, thus supporting a causal link between betaine and oxidative stress conditions. Furthermore, a CHDH variant, rs35518479, was identified as a cis-expression quantitative trait locus (QTL) for CHDH expression in postmortem brains from schizophrenia, allowing genotype-based stratification of schizophrenia patients for betaine efficacy. In conclusion, the present study underscores the potential benefit of betaine in a subset of schizophrenia.
Structural characterization of the PCV2d genotype at 3.3 Å resolution reveals differences to PCV2a and PCV2b genotypes, a tetranucleotide, and an N-terminus near the icosahedral 3-fold axes
Authors: Khayat Reza , Wen Ke , Alimova Aleksandra , Gavrilov Boris , Katz Al , Galarza Jose M. , Gottlieb J. Paul 
Published: April 2019

AbstractPorcine circovirus 2 (PCV2) is a T=1 non-enveloped icosahedral virus that has a major impact on the swine industry as an agent of porcine circovirus associate disease. PCV2 capsid protein sequences have been employed by others to provide a temporal description of the emerging genotypes. PCV2a is believed to be the earliest genotype and responsible for giving rise to PCV2b, which gives rise to PCV2d. The underlying mechanism responsible for the emerging genotypes is not understood. To determine if a change in the PCV2d capsid accompanies the emergence of this genotype, we determined the cryo-electron microscopy image reconstruction of PCV2d VLP at 3.3 Å resolution and compared it to the previously reported PCV2a and PCV2b structures. Differences between the CD and GH loops identify structural changes that accompany the emergence of PCV2b from PCV2a, and PCV2d from PCV2b. We also model additional amino acids for the N-terminus near the icosahedral 3-fold axes of symmetry and a tetranucleotide between the 5- and 2-fold axes of symmetry. To interpret the sequence diversity that defines the PCV2 genotypes on a structural platform we have performed structure-based sequence comparison. Our analysis demonstrates that each genotype possesses a unique set of amino acids located on the surface of the capsid that experience a high degree of substitution. These substitutions may be a response to the PCV2 vaccination program. The structural difference between PCV2a, b and d genotypes indicate that it is important to determine the PCV2 capsid structure as the virus evolves into different genotypes.ImportancePCV2 is a significant epidemic agricultural pathogen that is the causative agent of a variety of swine illnesses. PCV2 infections have significant economic impact in the swine industry and must be controlled by vaccination. Outbreaks in farms vaccinated for PCV2 suggest that improvements to the current vaccination programs are needed. Better understanding of the assembly, structure, replication and evolution of these viruses is necessary for production of improved vaccines. The ability of PCV2 to rapidly shift genotypes suggests that expression systems capable of rapidly producing large quantities of virus-like particles should be pursued. To these ends we have established a mammalian cell-based virus-like particle expression system and performed high resolution structural studies of a new PCV2 genotype. Differences between the structure of this genotype and earlier genotypes demonstrate that it is important to study the PCV2 structure as it shifts genotypes.
Age, Sex, and Genetics Influence the Abundance of Infiltrating Immune Cells in Human Tissues
Authors: Marderstein Andrew R. , Uppal Manik , Verma Akanksha , Bhinder Bhavneet , Mezey Jason , Clark Andrew G. , Elemento Olivier 
Published: April 2019

AbstractDespite infiltrating immune cells playing an essential role in human disease and the patient response to treatment, the central mechanisms influencing variability in infiltration patterns are unclear. Using bulk RNA-seq data from 53 GTEx tissues, we applied cell-type deconvolution algorithms to evaluate the immune landscape across the healthy human body. We first performed a differential expression analysis of inflamed versus non-inflamed samples to identify essential pathways and regulators of infiltration. Next, we found 21 of 73 infiltration-related phenotypes to be associated with either age or sex (FDR< 0.1). Through our genetic analysis, we discovered 13 infiltration-related phenotypes have genome-wide significant associations (iQTLs) (P< 5.0 × 10−8), with a significant enrichment of tissue-specific expression quantitative trait loci in suggested iQTLs (P< 10−5). We highlight an association between neutrophil content in lung tissue and a variant near theCUX1transcription factor gene (P= 9.7 × 10−11), which has been previously linked to neutrophil infiltration, inflammatory mechanisms, and the regulation of several immune response genes. Together, our results identify key factors influencing inter-individual variability of specific tissue infiltration patterns, which could provide insights on therapeutic targets for shifting infiltration profiles to a more favorable one.
Engineered Retroviruses as Fluorescent Biological Reference Particles for Small Particle Flow Cytometry
Authors: Tang Vera A. , Fritzsche Anna K. , Renner Tyler M. , Burger Dylan , van der Pol Edwin , Lannigan Joanne A. , Brittain George C. , Welsh Joshua A. , Jones Jennifer C. , Langlois Marc-André 
Published: April 2019

ABSTRACTThere has been renewed interest in the use of flow cytometry for single particle phenotypic analysis of particles in the nanometer size-range such as viruses, organelles, bacteria and extracellular vesicles (EVs). However, many of these particles are smaller than 200 nm in diameter, which places them at the limit of detection for many commercial flow cytometers. The use of reference particles of diameter, fluorescence, and light-scattering properties akin to those of the small biological particles being studied is therefore imperative for accurate and reproducible data acquisition and reporting across different instruments and analytical technologies. We show here that an engineered murine leukemia virus (MLV) can act as a fluorescence reference particle for other small particles such as retroviruses and EVs. More specifically, we show that engineered MLV is a highly monodisperse enveloped particle that can act as a surrogate to demonstrate the various effects of antibody labeling on the physical properties of small biological particles in a similar diameter range.
Sukhpal Dairy Farm: considering customer engagement-based growth options
Authors: Srivastava Ritu , Mangla Akhil 
Published: April 2019

Learning outcomes
The learning outcomes are as follows: understand the challenges and opportunities of an unorganized business/informal economy; compare and contrast the applications of customer engagement frameworks between small and big businesses; outline the steps in product design in a services context; discuss the services marketing mix as a part of the marketing strategy; and understand the need of scaling up the business operations in wake of new opportunity.

Case overview/synopsis
Sukhpal Dairy Farm (SDF) is a case of unorganized milk marketing in the Indian Emerging Market. Milk was sold as a commodity with a fragmented set of suppliers to only a small population. Changes in consumer demand, technology and supply chain presented huge opportunities to the small dairy farmer. But it was also a threat to him. The large corporater, players backed by strong logistics and supply chain operations support posed a big challenge to the small farmer who lacked scale and reach. Sukhpal, owner of SDF, struggled while considering the options to grow his business. He did not want to change the success factors of his existing business model that was based on word of mouth and customer engagement.

Complexity academic level
MBA students.

Supplementary materials
Teaching Notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes.

Subject code
CSS: 8: Marketing.
Research on map matching algorithm based on priority rule for low sampling frequency vehicle navigation data
Authors: Liu Zhishuo , Dongxin Yao , Kuan Zhao , Fang Wang Chun 
Published: April 2019

Purpose
There is a certain error in the satellite positioning of the vehicle. The error will cause the drift point of the positioning point, which makes the vehicle trajectory shift to the real road. This paper aims to solve this problem.

Design/methodology/approach
The key technology to solve the problem is map matching (MM). The low sampling frequency of the vehicle is far from the distance between adjacent points, which weakens the correlation between the points, making MM more difficult. In this paper, an MM algorithm based on priority rules is designed for vehicle trajectory characteristics at low sampling frequencies.

Findings
The experimental results show that the MM based on priority rule algorithm can effectively match the trajectory data of low sampling frequency with the actual road, and the matching accuracy is better than other similar algorithms, the processing speed reaches 73 per second.

Research limitations/implications
In the algorithm verification of this paper, although the algorithm design and experimental verification are considered considering the diversity of GPS data sampling frequency, the experimental data used are still a single source.

Originality/value
Based on the GPS trajectory data of the Ministry of Transport, the experimental results show that the accuracy of the priority-based weight-based algorithm is higher. The accuracy of this algorithm is over 98.1 per cent, which is better than other similar algorithms.
Perceived fairness of revenue management practices in casual and fine-dining restaurants
Authors: Tang Jason , Repetti Toni , Raab Carola 
Published: April 2019

PurposeRestaurants typically have small profit margins and with the pressure of increasing food and labor costs, management is looking to revenue as a way to maintain and drive profits. One technique to increase revenue is through revenue management practices, but management needs to be aware of their customers’ reactions to these practices prior to implementation. The paper aims to discuss this issue.Design/methodology/approachThis study utilizes linear regression to determine the impact of select restaurant revenue management practices, customers’ familiarity with revenue management in general and in restaurants specifically, and customers’ demographics on perceived fairness of revenue management practices in casual and fine-dining restaurants.FindingsResults indicate that customers find certain restaurant revenue management practices, such as charging premium prices on certain days of the week, fair in both casual and fine-dining restaurants, while others are not in either. Non-refundable reservation fees were found to be fair for fine-dining establishments only. Increased familiarity with restaurant revenue management was associated with higher perceptions of fairness for both casual and fine dining. Age was the only demographic studied that affected perceived fairness.Originality/valueThis study is the only known study to simultaneously evaluate the impact of price and duration restaurant revenue management techniques in combination with customer demographics and revenue management familiarity on consumer perceptions of fairness.
Micro‐ and macroparasite species richness in birds: The role of host life history and ecology
Authors: Gutiérrez Jorge S. , Piersma Theunis , Thieltges David W. 
Published: April 2019

Abstract

Identifying the factors shaping variation in parasite diversity among host species is crucial to understand wildlife diseases. Although micro‐ and macroparasites may exert different selective pressures on their hosts, studies investigating the determinants of parasite species richness in animals have rarely considered this divide.

Here, we investigated the role of host life history and ecology in explaining the species richness of helminths (macroparasites) and haemosporidians (microparasites) in birds world‐wide. We collated data from multiple global datasets on diverse bird traits (longevity, body mass, coloniality, migration distance/tendency, geographic range size and dietary and habitat breadths) and the species richness of their helminth and haemosporidian parasites. We tested predictors of helminth and haemosporidian parasite richness using phylogenetic generalized linear mixed models in a Bayesian framework.

We found that, after controlling for research effort and host phylogeny, the richness of helminths, but not of haemosporidians, increased with host longevity, range size, migration distance and dietary breadth. Overall, these correlates were also important across different helminth groups (acanthocephalans, cestodes, nematodes and trematodes), and two additional ones (body mass, coloniality) emerged as important for cestodes and acanthocephalans.

We propose that long life spans may promote the diversity of helminth parasite assemblages over evolutionary time, thus resulting in richer helminth faunas. Similarly, longer‐distance migrations, larger ranges and broader dietary breadths are likely to lead to greater encounter rates and the accumulation of trophically transmitted helminths. In contrast, vector‐borne haemosporidians may be influenced more by factors related to vector ecology than by the host traits included in the analyses. The lack of strong associations between haemosporidian species richness and host characteristics emphasizes the need to find appropriate traits to model the distribution and diversity of parasites with different environmental preferences in order to anticipate disease emergence risks associated with global change.
Does topsoil removal in grassland restoration benefit both soil nematode and plant communities?
Authors: Resch Monika Carol , Schütz Martin , Graf Ulrich , Wagenaar Roel , van der Putten Wim H. , Risch Anita C. 
Published: April 2019

Abstract

Successful restoration of semi‐natural grasslands on grasslands previously subject to intensive management needs to overcome manifold barriers. These include high soil fertility, the dominance of a few fast‐growing plant species, degraded soil faunal communities and missing propagules of the targeted above‐ and below‐ground flora and fauna. A combination of removing the topsoil and introducing propagules of target plants has become one of the major tools for nature conservation agencies and practitioners to reduce soil fertility and restore former species‐rich grasslands in various European countries.

Using topsoil removal as a restoration measure has provoked an ongoing debate between supporting nature conservation and rejecting soil protection agencies. Although it favours species‐rich plant communities, it strongly disturbs soil communities and affects physical and chemical soil properties and processes. Currently, there is a lack of long‐term data to assess how restored grassland ecosystems develop and recover after topsoil removal. Here, we used two well‐established bioindicators, soil nematodes and plants, to quantify restoration success of topsoil removal in comparison with alternative restoration measures and target communities 22 years after intervention.

The nematode community composition indicated reduced nutrient availability in the restored systems, as was aimed at by topsoil removal. Nevertheless, after this 22‐year period following topsoil removal, nematode composition and structure revealed successful recovery.

Plant communities benefitted from the reduction of soil nutrients after topsoil removal as indicated by higher numbers of plant species and higher Shannon diversity. Furthermore, topsoil removal strongly promoted the re‐establishment of plant species of the target plant community.

Synthesis and applications. Overall, our study demonstrates how a massive intervention by topsoil removal proved successful in converting intensively managed into species‐rich grasslands. This contrasts with the mild intervention of repeated mowing and removing of the harvested plant material. We show that, in the long run, potential negative effects of topsoil removal on the soil fauna can be successfully overcome and plant communities can develop into targeted species‐rich grassland.
Monitoring a Norwegian freshwater crayfish tragedy: eDNA snapshots of invasion, infection and extinction
Authors: Strand David A. , Johnsen Stein Ivar , Rusch Johannes C. , Agersnap Sune , Larsen William Brenner , Knudsen Steen Wilhelm , Møller Peter Rask , Vrålstad Trude 
Published: April 2019

Abstract

The European noble crayfish Astacus astacus is threatened by crayfish plague caused by the oomycete Aphanomyces astaci, which is spread by the invasive North American crayfish (e.g. signal crayfish Pacifastacus leniusculus). Surveillance of crayfish plague status in Norway has traditionally relied on the monitoring survival of cage‐held noble crayfish, a method of ethical concern. Additionally, trapping is used in crayfish population surveillance. Here, we test whether environmental DNA (eDNA) monitoring could provide a suitable alternative to the cage method, and a supplement to trapping.

We took advantage of an emerging crayfish plague outbreak in a Norwegian watercourse following illegal introduction of disease‐carrying signal crayfish, and initiated simultaneous eDNA monitoring and cage‐based surveillance, supplemented with trapping. A total of 304 water samples were filtered from several sampling stations over a 4‐year period. eDNA data (species‐specific quantitative real‐time PCR [qPCR]) for the presence of A. astaci, noble and signal crayfish within the water samples were compared to cage mortality and trapping.

This is the first study comparing eDNA monitoring and cage surveillance during a natural crayfish plague outbreak. We show that eDNA monitoring corresponds well with the biological status measured in terms of crayfish mortality and trapping results. eDNA analysis also reveals the presence of A. astaci in the water up to 2.5 weeks in advance of the cage method. Estimates of A. astaci and noble crayfish eDNA concentrations increased markedly during mortality and vanished quickly thereafter. eDNA provides a snapshot of the presence, absence or disappearance of crayfish regardless of season, and constitutes a valuable supplement to the trapping method that relies on season and legislation.

Synthesis and applications. Simultaneous eDNA monitoring of Aphanomyces astaci (crayfish plague) and relevant native and invasive freshwater crayfish species is well‐suited for early warning of invasion or infection, risk assessments, habitat evaluation and surveillance regarding pathogen and invasive/native crayfish status. This non‐invasive, animal welfare friendly method excludes the need for cage‐held susceptible crayfish in disease monitoring. Furthermore, eDNA monitoring is less likely to spread A. astaci than traditional methods. This study resulted in the implementation of eDNA monitoring for Norwegian crayfish plague and crayfish surveillance programmes, and we believe other countries could improve management strategies for freshwater crayfish using a similar approach.
Phenological mismatch between season advancement and migration timing alters Arctic plant traits
Authors: Choi Ryan T. , Beard Karen H. , Leffler A. Joshua , Kelsey Katharine C. , Schmutz Joel A. , Welker Jeffrey M. 
Published: April 2019

Abstract

Climate change is creating phenological mismatches between herbivores and their plant resources throughout the Arctic. While advancing growing seasons and changing arrival times of migratory herbivores can have consequences for herbivores and forage quality, developing mismatches could also influence other traits of plants, such as above‐ and below‐ground biomass and the type of reproduction, that are often not investigated.

In coastal western Alaska, we conducted a 3‐year factorial experiment that simulated scenarios of phenological mismatch by manipulating the start of the growing season (3 weeks early and ambient) and grazing times (3 weeks early, typical, 3 weeks late, or no‐grazing) of Pacific black brant (Branta bernicla nigricans), to examine how the timing of these events influence a primary goose forage species, Carex subspathacea.

After 3 years, an advanced growing season compared to a typical growing season increased stem heights, standing dead biomass, and the number of inflorescences. Early season grazing compared to typical season grazing reduced above‐ and below‐ground biomass, stem height, and the number of tillers; while late season grazing increased the number of inflorescences and standing dead biomass. Therefore, an advanced growing season and late grazing had similar directional effects on most plant traits, but a 3‐week delay in grazing had an impact on traits 3–5 times greater than a similarly timed shift in the advancement of spring. In addition, changes in response to treatments for some variables, such as the number of inflorescences, were not measurable until the second year of the experiment, while other variables, such as root productivity and number of tillers, changed the direction of their responses to treatments over time.

Synthesis. Factors affecting the timing of migration have a larger influence than earlier springs on an important forage species in the breeding and rearing habitats of Pacific black brant. The phenological mismatch prediction for this site of earlier springs and later goose arrival will likely increase above‐ and below‐ground biomass and sexual reproduction of the often‐clonally reproducing C. subspathacea. Finally, the implications of mismatch may be difficult to predict because some variables required successive years of mismatch to respond.