bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compartmentalization of the proteasome-interacting proteins during sperm capacitation
Authors: Zigo Michal , Manaskova-Postlerova Pavla , Jonakova Vera , Kerns Karl , Sutovsky Peter 
Published: August 2019

AbstractUbiquitination is a stable, reversible posttranslational modification of target proteins by covalent ligation of the small chaperone protein ubiquitin. Most commonly ubiquitination targets proteins for degradation/recycling by the 26S proteasome in a well-characterized enzymatic cascade. Studies using human and non-human mammalian spermatozoa revealed the role of the ubiquitin-proteasome system (UPS) in the regulation of fertilization, including sperm-zona pellucida (ZP) interactions as well as the early events of sperm capacitation, the remodeling of the sperm plasma membrane and acrosome, and for the acquisition of sperm fertilizing ability. The present study investigated the activity of UPS during in vitro capacitation of fresh boar spermatozoa in relation to changes in sperm proteome. Parallel and sequential treatments of ejaculated and capacitated spermatozoa under proteasome permissive/inhibiting conditions were used to isolate putative sperm proteasome-associated sperm proteins in a compartment-specific manner. A differential proteomic approach employing 1D PAGE revealed differences in accumulated proteins at the molecular weights of 60, 58, 49, and 35 kDa, and MS analysis revealed the accumulation of proteins previously reported as proteasome co-purifying proteins, as well as some novel proteins. Among others, P47/lactadherin, ACRBP, ADAM5, and SPINK2 (alias SAAI) were processed by the proteasome in a capacitation dependent manner. Furthermore, the capacitation-induced reorganization of the outer acrosomal membrane was slowed down in the presence of proteasomal inhibitors. These novel results support the proposed role of UPS in sperm capacitation and open several new lines of inquiry into sperm capacitation mechanism.
Summer high temperature extremes over Northeastern China predicted by spring soil moisture
Authors: Zhang Jingyong , Yang Zhanmei , Wu Lingyun , Yang Kai 
Published: August 2019

AbstractCurrent seasonal climate predictions mainly reside in the ocean anomalies. However, the prediction skills are generally limited over many extra-tropical land areas where the oceanic effects are relatively weak. In this study, we address the potential of preceding spring soil moisture condition to predict summer hot days over Northeastern China, a typical Northern Hemisphere mid-latitude region. The results show that spring soil moisture condition over Central-Eastern China is closely related with following summer hot days over Northeastern China for the period of 1979–2017. The statistical model based on the preceding spring soil moisture condition yields temporal cross-validated correlation skill of 0.57 for summer hot days over Northeastern China. The spatial pattern correlation skills of independent hindcast experiments for 2009–2017 are also high, ranging from 0.87 to 0.94. Our results can be easily applied to practical prediction of summer hot days over Northeastern China, and help to provide better climate services and reduce the detrimental effects of extreme heat over this extra-tropical region.
Impact of Resolution of Hyponatremia on Neurocognitive and Motor Performance in Geriatric Patients
Authors: Brinkkoetter Paul Thomas , Grundmann Franziska , Ghassabeh Panteha Jazayeri , Becker Ingrid , Johnsen Marc , Suaréz Victor , Schulz Ralf-Joachim , Streichert Thomas , Burst Volker 
Published: August 2019

AbstractThis observational study investigated the impact of hyponatremia resolution on the results of a comprehensive geriatric assessment (CGA) in 150 patients with age ≥70 years and serum sodium <130 mEq/L. The test battery including Barthel index of Activities of Daily Living (ADL) and various tests of neurocognitive function, motor performance and mood stability was applied on admission and at discharge. Changes of individual test results (Δ) were analyzed and normonatremic patients matched for age, gender, and ADL served as reference group. Most CGA test results improved. The improvement was more pronounced in the hyponatremia group with respect to ADL (ΔADL: 14.3 ± 17.1 vs. 9.8 ± 14.7; p = 0.002) and MMSE (ΔMMSE: 1.8 ± 3.0 vs. 0.7 ± 1.9; p = 0.002). Effect sizes were small (i.e., >0.2) in the overall analysis for ΔADL and ΔMMSE and moderate (i.e., >0.5) for ΔMMSE in the euvolemic subgroup. Beneficial effects on ΔADL and ΔMMSE were only observed in the subgroup of patients in which [Na+] was raised by >5 mEq/L and multivariable linear regression analysis confirmed [Na+] increase to be an independent predictor of MMSE improvement. Resolution of hyponatremia has a beneficial impact on the geriatric patients’ overall functional status, in particular in euvolemic cases.
18F-FDG Positron-Emission Tomography/Computed Tomography Findings of Radiographic Lesions Suggesting Old Healed Pulmonary Tuberculosis and High-risk Signs of Predicting Recurrence: A Retrospective Study
Authors: Ji Yu , Shao Chunchun , Cui Yong , Shao Guangrui , Zheng Jingsong 
Published: August 2019

AbstractPulmonary tuberculosis (PTB) is a common worldwide infection with high mortality and morbidity, especially in developing countries. This study analyzed PET/CT findings in tumor patients with radiographic lesions suggesting old healed pulmonary tuberculosis (OHPTB) and imaging follow-up to find and verify PET/CT signs that may predict tuberculosis recurrence. A retrospective analysis of the tumor patients was carried out. These patients underwent 18F-FDG PET/CT in our center from 2010 to 2018. Confirmation of tuberculosis recurrence was obtained by follow-up of morphological changes in old lesions by PET/CT or CT. In total, 238 patients with a complete medical history were included in the final study, and 22 patients experienced OHPTB recurrence. We found that the SUVmax of tuberculosis in PET/CT was significantly increased in the recurrence group compared to the non-recurrence group [5.00 (3.40, 7.30) vs. 1.10 (0.80, 1.30), P < 0.001]. The ROC curve showed good discrimination, with an AUC of 0.980, and a cut-off SUVmax value of 2.15 was identified (the sensitivity was 90.5%, the specificity was 97.2%, the positive predictive value was 76.0%, and the negative predictive value was 99.1%). Both the tumor and the anti-tumor treatment can cause the patient to be immunocompromised and might further cause the recurrence of OHPTB. Positive imaging on 18F-FDG PET can predict the recurrence of OHPPT. Although there might be a false positive, 18F-FDG PET can greatly narrow the monitoring range. A negative result on imaging has high reliability for eliminating the possibility of tuberculosis recurrence. PET/CT has important clinical significance in tuberculosis management in patients with concurrent OHPTB.
Carcinogenic risk of human papillomavirus (HPV) genotypes and potential effects of HPV vaccines in Korea
Authors: Park Eunhyang , Kim Ji-Ye , Choi Sangjoon , Kim Dae Shick , Oh Young Lyun 
Published: August 2019

AbstractThis study investigated the distribution of HPV types in Korean women and evaluated the carcinogenic risk of individual HPV types and the potential effects of HPV vaccines. A total of 4,081 HPV-positive samples between 2014 and 2017 were included. The most prevalent genotypes were HPV 16, 58, 68, and 56. Among them, HPV 16 was significantly higher in high-grade squamous intraepithelial neoplasia or worse (HSIL+ ) group. In cytologically evaluating the risk for HSIL+ by individual HPV types, HPV 16 was associated with the highest risk of HSIL+ (OR = 10.82; 95% CI: 7.93–14.77), followed by HPV 33, 31, 52, 18, 58, 51, and 35, in descending order (OR = 3.50 [type 33] to 2.62 [type 35]). Among those types, HPV 16, 18, 31, 33, and 58 were also significantly associated with HSIL+ on histologic evaluation. The analysis of the HPV subgroups covered by the different vaccines revealed that the HPV types covered by the 9-valent vaccine had a high association with HSIL+ (OR = 4.09; 95% CI: 3.02–5.54). Our findings highlight the different carcinogenic risks posed by the high risk HPV genotypes and the positive potential effects of the 9-valent HPV vaccine in reducing HPV-associated cervical cancer in Korea.
Cross-reactivity of sIgE to mite and shrimp induced allergies in different age groups and clinical profiles of shrimp sIgE in vegetarians
Authors: Shen Cheng-Ying , Tsai Jaw-Ji , Liao En-Chih 
Published: August 2019

AbstractThe sensitization to house dust mites (HDMs) and shrimps affects the development of hypersensitivity with an increase in age. Due to the cross-reactivity between shellfish and HDMs, HDMs were considered as the primary sensitizer for shellfish allergy. Thus, vegetarians might be sensitized to shrimp through the inadvertent inhalation of HDMs. Therefore, we assessed the prevalence of shrimp or mite allergy among different age groups and vegetarians. The serum specific-IgE (sIgE) level of HDMs and shrimp in 60 children/adolescence (un-adults), 30 adults, 30 elderly, and four vegetarian adults patients were measured. The sera with sIgE levels greater than 3.5 kUA/L were cross-reactivity examined. We found that HDMs induced higher sIgE than shrimp in un-adults. In contrast, shrimp-induced sIgE was higher in the adults and elderly patients. Moreover, adults were more frequently sensitized to shrimp and mite at the same time compared with the un-adult or elderly groups. The mite-Der p 10 not only displayed high cross-reactivity to the shrimp-Pen a 1 in all age groups and vegetarians but functioned as the major allergen to sensitize un-adults. Overall, the level of mite or shrimp sIgE is influenced by alterations in age, and vegetarians are at risk of shrimp sensitization via cross-reactivity between shrimp and mite.
Thermal Perceptual Thresholds are typical in Autism Spectrum Disorder but Strongly Related to Intra-individual Response Variability
Authors: Williams Zachary J. , Failla Michelle D. , Davis Samona L. , Heflin Brynna H. , Okitondo Christian D. , Moore David J. , Cascio Carissa J. 
Published: August 2019

AbstractIndividuals with autism spectrum disorder (ASD) are often reported to exhibit an apparent indifference to pain or temperature. Leading models suggest that this behavior is the result of elevated perceptual thresholds for thermal stimuli, but data to support these assertions are inconclusive. An alternative proposal suggests that the sensory features of ASD arise from increased intra-individual perceptual variability. In this study, we measured method-of-limits warm and cool detection thresholds in 142 individuals (83 with ASD, 59 with typical development [TD], aged 7–54 years), testing relationships with diagnostic group, demographics, and clinical measures. We also investigated the relationship between detection thresholds and a novel measure of intra-individual (trial-to-trial) threshold variability, a putative index of “perceptual noise.” This investigation found no differences in thermal detection thresholds between individuals with ASD and typical controls, despite large differences between groups in sensory reactivity questionnaires and modest group differences in intra-individual variability. Lower performance IQ, male sex, and higher intra-individual variability in threshold estimates were the most significant predictors of elevated detection thresholds. Although no psychophysical measure was significantly correlated with questionnaire measures of sensory hyporeactivity, large intra-individual variability may partially explain the elevated psychophysical thresholds seen in a subset of the ASD population.
Side effects of toxic bait formulations on Diachasmimorpha longicaudata (Hymenoptera: Braconidae)
Authors: Bernardi Daniel , Nondillo Aline , Baronio Cléber Antonio , Bortoli Lígia Caroline , Junior Ruben Machota , Treptow Rute Caroline Becker , Geisler Fernanda Carla Santos , Neitzke Camila Gauger , Nava Dori Edson , Botton Marcos 
Published: August 2019

AbstractDiachasmimorpha longicaudata (Ashmead, 1905) (Hymenoptera: Braconidae) is considered one of the main biological control agents of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). However, the application of toxic baits for the management of C. capitata might exert side effects on the parasitoid. The objective of this study was to evaluate the side effects of toxic bait formulations on D. longicaudata. The food attractants Anamed, 3% Biofruit, 1.5% CeraTrap, 1.25% Flyral, 3% Isca Samaritá, 3% Isca Samaritá Tradicional, and 7% sugarcane molasses mixed with an organophosphate insecticide [malathion, 2.0 grams of active ingredient (g a.i.) L−1] and the commercial formulation Gelsura (2.0 and 4.0 g a.i. L−1 alpha-cypermethrin) showed high toxicity to D. longicaudata adults (>90% mortality) after 96 h and were thus classified as harmful (Class 4). Similarly, 3% Isca Samaritá Tradicional and 7% sugarcane molasses in formulations with the insecticides spinosad and spinetoram (0.096 g a.i. L−1 or kg) were moderately harmful (Class 3). In contrast, the food attractants Anamed, 3% Biofruit, 1.5% CeraTrap, 1.25% Flyral, and 3% Isca Samaritá Tradicional in combination with spinosad and spinetoram and the formulation Success 0.02CB (0.096 g a.i. L−1 spinosad) were classified as harmless (<10% mortality up to 96 h, Class 1). Additionally, these formulations did not reduce the parasitism and emergence rate of the F1 generation of D. longicaudata in C. capitata larvae. Formulations of toxic baits based on spinosyn are suitable for the management of C. capitata together with the parasitoid D. longicaudata.
Cold adaptation in the environmental bacterium Shewanella oneidensis is controlled by a J-domain co-chaperone protein network
Authors: Maillot Nathanael Jean , Honoré Flora Ambre , Byrne Deborah , Méjean Vincent , Genest Olivier 
Published: August 2019

AbstractDnaK (Hsp70) is a major ATP-dependent chaperone that functions with two co-chaperones, a J-domain protein (JDP) and a nucleotide exchange factor to maintain proteostasis in most organisms. Here, we show that the environmental bacterium Shewanella oneidensis possesses a previously uncharacterized short JDP, AtcJ, dedicated to cold adaptation and composed of a functional J-domain and a C-terminal extension of 21 amino acids. We showed that atcJ is the first gene of an operon encoding also AtcA, AtcB and AtcC, three proteins of unknown functions. Interestingly, we found that the absence of AtcJ, AtcB or AtcC leads to a dramatically reduced growth at low temperature. In addition, we demonstrated that AtcJ interacts via its C-terminal extension with AtcC, and that AtcC binds to AtcB. Therefore, we identified a previously uncharacterized protein network that involves the DnaK system with a dedicated JDP to allow bacteria to survive to cold environment.
Liquid–liquid phase separation morphologies in ultra-white beetle scales and a synthetic equivalent
Authors: Burg Stephanie L. , Washington Adam , Coles David M. , Bianco Antonino , McLoughlin Daragh , Mykhaylyk Oleksandr O. , Villanova Julie , Dennison Andrew J. C. , Hill Christopher J. , Vukusic Pete , Doak Scott , Martin Simon J. , Hutchings Mark , Parnell Steven R. , Vasilev Cvetelin , Clarke Nigel , Ryan Anthony J. , Furnass Will , Croucher Mike , Dalgliesh Robert M. , Prevost Sylvain , Dattani Rajeev , Parker Andrew , Jones Richard A. L. , Fairclough J. Patrick A. , Parnell Andrew J. 
Published: August 2019

AbstractCyphochilus beetle scales are amongst the brightest structural whites in nature, being highly opacifying whilst extremely thin. However, the formation mechanism for the voided intra-scale structure is unknown. Here we report 3D x-ray nanotomography data for the voided chitin networks of intact white scales of Cyphochilus and Lepidiota stigma. Chitin-filling fractions are found to be 31 ± 2% for Cyphochilus and 34 ± 1% for Lepidiota stigma, indicating previous measurements overestimated their density. Optical simulations using finite-difference time domain for the chitin morphologies and simulated Cahn-Hilliard spinodal structures show excellent agreement. Reflectance curves spanning filling fraction of 5-95% for simulated spinodal structures, pinpoint optimal whiteness for 25% chitin filling. We make a simulacrum from a polymer undergoing a strong solvent quench, resulting in highly reflective (~94%) white films. In-situ X-ray scattering confirms the nanostructure is formed through spinodal decomposition phase separation. We conclude that the ultra-white beetle scale nanostructure is made via liquid–liquid phase separation.
Speech Production From a Developmental Perspective
Authors: Redford Melissa A. 
Published: August 2019

PurposeCurrent approaches to speech production aim to explain adult behavior and so make assumptions that, when taken to their logical conclusion, fail to adequately account for development. This failure is problematic if adult behavior can be understood to emerge from the developmental process. This problem motivates the proposal of a developmentally sensitive theory of speech production. The working hypothesis, which structures the theory, is that feedforward representations and processes mature earlier than central feedback control processes in speech production.MethodTheoretical assumptions that underpin the 2 major approaches to adult speech production are reviewed. Strengths and weaknesses are evaluated with respect to developmental patterns. A developmental approach is then pursued. The strengths of existing theories are borrowed, and the ideas are resynthesized under the working hypothesis. The speech production process is then reimagined in developmental stages, with each stage building on the previous one.ConclusionThe resulting theory proposes that speech production relies on conceptually linked representations that are information-reduced holistic perceptual and motoric forms, constituting the phonological aspect of a system that is acquired with the lexicon. These forms are referred to as exemplars and schemas, respectively. When a particular exemplar and schema are activated with the selection of a particular lexical concept, their forms are used to define unique trajectories through an endogenous perceptual–motor space that guides implementation. This space is not linguistic, reflecting its origin in the prespeech period. Central feedback control over production emerges with failures in communication and the development of a self-concept.
Back From the Future: Nonlinear Anticipation in Adults' and Children's Speech
Authors: Noiray Aude , Wieling Martijn , Abakarova Dzhuma , Rubertus Elina , Tiede Mark 
Published: August 2019

PurposeThis study examines the temporal organization of vocalic anticipation in German children from 3 to 7 years of age and adults. The main objective was to test for nonlinear processes in vocalic anticipation, which may result from the interaction between lingual gestural goals for individual vowels and those for their neighbors over time.MethodThe technique of ultrasound imaging was employed to record tongue movement at 5 time points throughout short utterances of the form V1#CV2. Vocalic anticipation was examined with generalized additive modeling, an analytical approach allowing for the estimation of both linear and nonlinear influences on anticipatory processes.ResultsBoth adults and children exhibit nonlinear patterns of vocalic anticipation over time with the degree and extent of vocalic anticipation varying as a function of the individual consonants and vowels assembled. However, noticeable developmental discrepancies were found with vocalic anticipation being present earlier in children's utterances at 3–5 years of age in comparison to adults and, to some extent, 7-year-old children.ConclusionsA developmental transition towards more segmentally-specified coarticulatory organizations seems to occur from kindergarten to primary school to adulthood. In adults, nonlinear anticipatory patterns over time suggest a strong differentiation between the gestural goals for consecutive segments. In children, this differentiation is not yet mature: Vowels show greater prominence over time and seem activated more in phase with those of previous segments relative to adults.
Evolution of Speech: Anatomy and Control
Authors: de Boer Bart 
Published: August 2019

PurposeThis article critically reviews work on the evolution of speech in the context of motor control. It presents a brief introduction to the field of language evolution, of which the study of the evolution of speech is an integral component, and argues why taking the evolutionary perspective is useful. It then proceeds to review different methods of studying evolutionary questions: comparative research, experimental and observational research, and computer and mathematical modeling.ConclusionsOn the basis of comparative analysis of related species (specifically, other great apes) and on the basis of theoretical results, this article argues that adaptations for speech must have evolved gradually and that it is likely that speech motor control is one of the key aspects that has undergone observable selection related to speech, because, in this area, all the necessary precursors are present in closely related species. This implies that it must be possible to find empirical evidence for how speech evolved in the area of speech motor control. However, such research is only in its infancy at the present moment.
Assessment of Childhood Apraxia of Speech: A Review/Tutorial of Objective Measurement Techniques
Authors: Terband Hayo , Namasivayam Aravind , Maas Edwin , van Brenk Frits , Mailend Marja-Liisa , Diepeveen Sanne , van Lieshout Pascal , Maassen Ben 
Published: August 2019

BackgroundWith respect to the clinical criteria for diagnosingchildhood apraxia of speech(commonly defined as a disorder of speech motor planning and/or programming), research has made important progress in recent years. Three segmental and suprasegmental speech characteristics—error inconsistency, lengthened and disrupted coarticulation, and inappropriate prosody—have gained wide acceptance in the literature for purposes of participant selection. However, little research has sought to empirically test the diagnostic validity of these features. One major obstacle to such empirical study is the fact that none of these features is stated in operationalized terms.PurposeThis tutorial provides a structured overview of perceptual, acoustic, and articulatory measurement procedures that have been used or could be used to operationalize and assess these 3 core characteristics. Methodological details are reviewed for each procedure, along with a short overview of research results reported in the literature.ConclusionThe 3 types of measurement procedures should be seen as complementary. Some characteristics are better suited to be described at the perceptual level (especially phonemic errors and prosody), others at the acoustic level (especially phonetic distortions, coarticulation, and prosody), and still others at the kinematic level (especially coarticulation, stability, and gestural coordination). The type of data collected determines, to a large extent, the interpretation that can be given regarding the underlying deficit. Comprehensive studies are needed that include more than 1 diagnostic feature and more than 1 type of measurement procedure.