bluuebunny/crossref_metadata_2025_split huggingface dataset:
The oncolytic efficacy and safety of avian reovirus and its dynamic distribution in infected mice
Authors: Cai Ruimin , Meng Guangyuan , Li Yi , Wang Wenyang , Diao Youxiang , Zhao Shuping , Feng Qiang , Tang Yi 
Published: July 2019

Primary liver cancer is a major public health challenge that ranks as the third most common cause of cancer worldwide despite therapeutic improvement. Reovirus has been emerging as a potential anti-cancer agent and is undergoing multiple clinical trials, and it is reported that reovirus can preferentially cause the cell death of a variety of cancers in a manner of apoptosis. As few studies have reported the efficacy of oncolytic activity and safety profile of avian reovirus, in our study, LDH assay, MTT assay, DAPI staining, and flow cytometry assay were performed to demonstrate the oncolytic effects of avian reovirus against the HepG2 cells, and quantitative real-time PCR (qRT-PCR) and animal experiments were conducted to investigate the dynamic distribution of avian reovirus in infected mice and then illustrate the safety and tissue tropism of avian reovirus. LDH assay, DAPI staining, and flow cytometry assay confirmed the efficacy of the oncotherapeutic effects of avian reovirus, and MTT assay has indicated that avian reovirus suppressed the proliferation of HepG2 cells and decreased their viability significantly. qRT-PCR revealed the dynamic distribution of avian reovirus in infected mice that avian reovirus might replicate better and have more powerful oncolytic activity in liver, kidney, and spleen tissues. Furthermore, histopathological examination clearly supported that avian reovirus appeared non-pathogenic to the normal host, so our study may provide the new insights and rationale for the new strategy of removing liver cancer. Impact statement We demonstrated the efficacy of oncolytic activity of avian reovirus (ARV) by LDH assay, MTT assay, DAPI staining, and flow cytometry assay, and also investigated the dynamic distribution of ARV in infected mice and then illustrated the safety and tissue tropism of ARV by quantitative real-time PCR (qRT-PCR) and animal experiments. Collectively, our study may provide the new insights and rationale for the new strategy of removing liver cancer.
Pathogenic mutations in genes encoding nuclear envelope proteins and defective nucleocytoplasmic connections
Authors: Östlund Cecilia , Chang Wakam , Gundersen Gregg G , Worman Howard J 
Published: July 2019

Mutations in genes encoding nuclear lamins and associated nuclear envelope proteins have been linked to a broad range of inherited diseases affecting different tissues and organs. These diseases are often referred to as laminopathies. Scientists have yet to elucidate exactly how pathogenic mutations leading to alteration of a nuclear envelope protein cause disease. Our relatively recent research has shown that pathogenic mutations in genes encoding nuclear envelope proteins lead to defective nucleocytoplasmic connections that disrupt proper functioning of the linker of nucleoskeleton and cytoskeleton complex in the establishment of cell polarity. These defects may explain, at least in part, pathogenic mechanisms underlying laminopathies.Impact statementMutations in genes encoding nuclear lamins and associated nuclear envelope proteins have been linked to several diseases affecting different tissues and organs. The pathogenic mechanisms underlying these diseases, often called laminopathies, remain poorly understood. Increased knowledge of the functions of different nuclear envelope proteins and the interactions between them is crucial to elucidate these disease mechanisms. Our research has shown that pathogenic mutations in genes encoding nuclear envelope proteins lead to defective nucleocytoplasmic connections that disrupt proper functioning of the linker of nucleoskeleton and cytoskeleton (LINC) complex in the establishment of cell polarity. These defects may contribute to the pathogenesis of laminopathies and provide novel targets for therapeutics.
Drug change: ‘a hassle like no other’. An in-depth investigation using the Danish patient safety database and focus group interviews with Danish hospital personnel
Authors: Poulsen Joo Hanne , Rishøj Rikke Mie , Fischer Hanne , Kart Trine , Nørgaard Lotte Stig , Sevel Christian , Dieckmann Peter , Clemmensen Marianne Hald 
Published: July 2019

Background: Drug change (DC) is a common challenge in Danish hospitals. It affects the work of hospital personnel and has potentially serious patient safety consequences. Focus on medication safety is becoming increasingly important in the prevention of adverse events. The aim of this study is to identify and describe patient safety challenges related to DCs, and to explore potential facilitators to improve patient safety in the medication process in Danish hospital setting. Method: Two qualitative methods were combined. Data were obtained from the Danish Patient Safety Database (DPSD) containing incidents reports of adverse events related to DCs. Additionally, five semi-structured focus group interviews with hospital personnel (doctors, nurses, pharmacists and pharmacy technicians) from the five regions of Denmark were held. Results: The DPSD search identified 88 incidents related to DCs due to tender or drug shortage. The incidents were linked to prescribing errors, incorrect dose being dispensed/administered, and delayed/omitted treatment. Four themes from the interviews emerged: (1) challenges related to the drug itself; (2) situational challenges; (3) challenges related to the organization/IT systems/personnel; (4) facilitators/measures to ensure patient safety. Conclusion: DC is as a complex challenge, especially related to drug shortage. The results allow for a deeper understanding of the challenges and possible facilitators of DCs on the individual and organizational level. Pharmacy personnel were identified to play a key role in ensuring patient safety of DCs in hospitals. Indeed, this emphasizes that pharmacy personnel should be engaged in developing patient safety strategies and support hospital personnel around drug changes.
Participant experiences in a paraprofessional-led weight management program for children and families
Authors: Saxe-Custack Amy , Kerver Jean M. , Mphwanthe Getrude , Weatherspoon Lorraine 
Published: July 2019

Background: Pediatric obesity is a serious and widespread medical condition that is increasing in the United States. Unfortunately, family-based programming to address the disorder fails to successfully reach and engage many children, particularly in low-income communities. To provide more affordable, accessible, and scalable programming options, researchers partnered with pediatricians and the Cooperative Extension Service (Extension) in a Midwestern state to develop a collaborative intervention. Partnering pediatricians referred children and families to a weight management program delivered by a trained Extension paraprofessional. The current study describes family experiences with the program. Methods: Researchers conducted a focus group or family interview with 13 program completers to elicit program perceptions, experiences with paraprofessionals, and motivators to continue. The focus group and family interviews were audio recorded and transcribed verbatim for textual analysis. Using thematic analysis, researchers examined patterns across transcripts and formulated emerging themes. Results: Key themes that emerged included (1) nutrition guidance, (2) interaction, (3) child influence, and (4) family engagement. Families viewed paraprofessionals as compassionate and competent educators who were instrumental in helping families modify health-related behaviors. Conclusions: Results of the current study are important to efforts focused on addressing childhood obesity, particularly in underserved communities where access to healthcare services is limited.
Rare genetic variants in patients with cervical artery dissection
Authors: Traenka Christopher , Kloss Manja , Strom Tim , Lyrer Philippe , Brandt Tobias , Bonati Leo H , Grond-Ginsbach Caspar , Engelter Stefan 
Published: July 2019

Introduction The potential role of genetic alterations in cervical artery dissection (CeAD) pathogenesis is poorly understood. We aimed to identify pathogenic genetic variants associated with cervical artery dissection by using whole exome sequencing. Patients and methods CeAD-patients with either a family history of cervical artery dissection (f-CeAD) or recurrent cervical artery dissection (r-CeAD) from the CeAD-databases of two experienced stroke centres were analysed by whole exome sequencing. Variants with allele frequency <0.05 and classified as pathogenic by predicting algorithms (SIFT or Polyphen-2) or the ClinVar database were explored. First, we analysed a panel of 30 candidate genes associated with arterial dissection (any site) or aneurysm according to the OMIM (online Mendelian Inheritance of Men) database. Second, we performed a genome-wide search for pathogenic variants causing other vascular phenotypes possibly related to cervical artery dissection. Findings were classified as CeAD-causing (pathogenic variants in genes from the arterial dissection or aneurysm panel) or suggestive (pathogenic variants in genes associated with other vascular phenotypes and variants of unknown significance in genes from the arterial dissection or aneurysm panel). All other variants were classified as benign/uncertain. Results Among 43 CeAD-patients, 28 patients (17 pedigrees) had f-CeAD and 15 had r-CeAD. No CeAD-causing variants were identified in r-CeAD patients. Among f-CeAD-patients, 5/17 pedigrees carried CeAD-causing variants in COL3A1, COL4A1, COL4A3, COL4A4, COL5A1, COL5A2 and FBN1. Suggestive variants in ABCC6, COL3A1, COL5A2, MEF2A, and RNF213 were detected in three pedigrees with f-CeAD and six patients with r-CeAD. Discussion and conclusion: CeAD-causing variants were rare and exclusively found in f-CeAD-patients, suggesting differences between the genetic architectures of f-CeAD and r-CeAD. The identified variants indicate a high genetic heterogeneity of the study sample.
We need to talk: Provider conversations with peers and patients about a medical error
Authors: Dhawale Tejaswini , Zech Jennifer , Greene Sarah M , Roblin Douglas W , Brigham Karen Berg , Gallagher Thomas H , Mazor Kathleen M 
Published: July 2019

Purpose Although open communication with patients is the established best practice after a medical error, healthcare providers’ conversations with each other in these circumstances are less studied. We identified and compared what providers identified as the most important thing to say to their peer and to the patient after a medical error. Methods and materials: This study surveyed providers about the most important thing they would say to their peers and patient regarding a hypothetical scenario depicting a delayed diagnosis of cancer. Participants included primary care physicians, oncologists, and oncology nurses. Direct content analysis was used to identify major themes and the McNemar test was used to evaluate significant differences in the providers’ references to major themes (p > 0.05). Results A total of 303 providers produced valid responses. Four major themes emerged: (1) information sharing; (2) emotion handling; (3) preventing recurrences; and (4) responsibility. While the majority of provider responses included information sharing, fewer than one-third described the event as an error. Significantly, fewer providers addressed emotion with their peer than with the patient (10% vs. 54%, p ≤ 0.001). Providers were more likely to bring up prevention of recurrences with their peer than with the patient (43% vs. 19%, p ≤ 0.001). Approximately one-quarter of providers addressed responsibility with the peer and patient (25% vs. 26%, p = 0.707), although fewer than 10% acknowledged personal responsibility for the error in either context. Conclusion Providers approach conversations about medical errors with a peer differently than with patients and may benefit from additional communication training or support.
An exploration of providing mental health skills in a generic advanced clinical practice programme
Authors: Chadwick Angelina , Murphy Neil 
Published: July 2019

Background: advanced clinical practitioners (ACPs) are expected to be competent in their holistic assessment and management of individuals, which includes those with both physical and mental health problems. A mental health component was introduced within a generic advanced practitioner programme to support the development of mental health skills required by advanced clinical practitioners in training (ACPiTs). Aims: this research investigated the efficacy of content specific to mental health within an MSc ACP generic programme. Methods: a single case study approach was adopted, which used a purposive sample of 10 ACPiTs to explore personal beliefs and experiences using semistructured interviews. Verbatim transcription was undertaken followed by content and thematic analysis. Findings: Themes emerged included communication skills, and increased competence and self-awareness. Conclusion: insights provided by the ACPiTs showed they recognised the value of mental health teaching and exposure within their training programme in advancing their knowledge and skillset and, ultimately, increasing confidence in their clinical practice.
Menyikapi Fenomena Labelling Olok-olok Politik di Media Sosial Perspektif Hadis Nabi
Authors: Andi Azhari 
Published: July 2019

This article discusses the case of social labelling in social media in relation to recent political issues. This case is increasing among Indonesian people since 2014 general election up to date which gives bad effects to society. This article explains the case in more detail by using social sciences theories. Then it takes hadith as inspiration to solve the case by using paradigm method which is introduced by Kuntowijoyo. Users of netizen, practically, collaborates both of hadith and alquran to get comprehensive argument. In short, this article finds some ways to face the case of labelling in social media in relation to politic issues, people are communicating respectly with others; keeping good attitude in social media includes avoiding to give bad titles (labelling) to others; saying or doing nothing for unable to say or do good things; and supporting the candidates wisely to reject “fanaticsm”. AbstrakArtikel ini mendiskusikan problem sosial tentang labelling olok-olok politik di media sosial yang massif terjadi dewasa ini di tengah-tengah hiruk pikuk menyambut pesta demokrasi akbar Indonesia 2019. Gegap gempita masyarakat Indonesia dalam menjamu momentum “gadang” tersebut sangat luar biasa. Antusiasme tersebut patut diacungkan jempol. Namun di sisi lain, antusiasme berlebihan tersebut justru menimbulkan kegaduhan di media sosial yang berdampak di kehidupan nyata. Sebut saja perilaku labelling olok-olok politik antara simpatisan pendukung dari partai dan calon pemimpin. Perilaku labelling ini pada gilirannya menimbulkan gesekan dan perpecahan di masyarakat. Artikel inimeninjau fenomena tersebut dari perspektif ilmu sosial (labelling theory, sensate culture theory dan tindakan sosial). Kemudian tulisan ini menggali inspirasi-inspirasi hadis nabi untuk menyikapi fenomena ini dengan pendekatan teori Paradigma Kuntowijoyo di mana hadis dijadikan sebagai paradigma. Dalam menyikapi labelling olok-olok politik di media social artikel ini menemukanetika baik dalam berkomunikasi seperti memberikan label atau julukan yang baik, berkata positif, diam, dan mendukung (mencintai calon pemimpin) sewajaranya agar tidak jatuh ke dalam sikap fanatisme buta.
Evaluate the existing political oversight role of Turkish parliament over the public finance and in this context analyzed the new Turkish Presidential Government System
Authors: Biçer Mustafa 
Published: July 2019

Turkey changed its 1982 Constitution and adopted a presidential form of government in 2017. This constitutional amendment changed the approval of the budget radically. Along with that, debates may arise in terms of political responsibility for not approved the budget by the Parliament, how the budget will accepted when the president and majority of the Parliament are from different party, and there is not any mechanism being in classical presidential system as public account committee to enhance parliamentary supervision over the executive branch.In this study,- we deliberate the concept of political power in representative democracy in terms of public decision-making and resource allocation process and then we analyze the existing political oversight role of Turkish Parliament over the public finance on the basis of democratic legitimacy of public decision-making process and political accountability. - we focus on oversight role of Turkish Parliament over the public finance at the point of using "power of purse after the Constitutional Amendment adopted on April 16, 2017. Within the context of this analysis, the new constitutional structure of Parliament's budget approval and audit process in Turkish Parliament was evaluated.
Avaliação da atividade antifúngica do óleo essencial de Pogostemon cablin (Blanco) Benth. (Lamiaceae) contra cepas de Candida glabrata
Authors: Pimenta Eduardo , Da Cruz Renata , Diniz-Neto Hermes , Silva Daniele , De Oliveira Heloísa , Buccini Danieli , Lima Edeltrudes , De Oliveira Filho Abrahão 
Published: July 2019

A candidíase invasiva é uma ameaça crescente entre pacientes imunocomprometidos, sendo uma causa significativa de morbidade e mortalidade. Epidemiologicamente, as espécies não-albicans estão adquirindo relevância por se tornarem os agentes causadores cada vez mais frequentes de candidíase. O uso indiscriminado de medicamentos para combater as diversas variedades de candidíase tem trazido um aumento expressivo no número de leveduras resistentes aos antifúngicos tradicionais. Desta forma, produtos de origem vegetal podem ser utilizados como alternativa para os antifúngicos sintéticos. Com base nestas informações, esta pesquisa objetivou avaliar a possível atividade antifúngica do óleo essencial de Pogostemon cablin (Blanco) Benth. (Lamiaceae) contra cepas de Candida glabrata através da técnica de microdiluição para a determinação da Concentração Inibitória Mínima (CIM) e Concentração Fungicida Mínima (CFM). Após o experimento, observou-se que o produto inibiu a cepa LM-302 de C. glabrata com uma CIM de 256 μg/mL. O mesmo valor foi obtido para a CFM contra esta mesma cepa. Portanto, pode-se concluir que o óleo essencial de P. cablin testado apresenta um efeito antifúngico contra as leveduras de C. glabrata.
Avaliação qualitativa das preparações de cardápios de unidades de alimentação e nutrição hospitalares em Sergipe
Authors: Lima Talita Silva , Wartha Elma Regina Silva Silva Andrade , Souza Anaxágora Conceição , Carvalho Izabela Maria Montezano de 
Published: July 2019

O presente trabalho objetivou avaliar a qualidade nutricional dos cardápios ofertados a funcionários e acompanhantes de pacientes em Unidades de Alimentação e Nutrição Hospitalares, localizadas no estado de Sergipe. Foram analisados os cardápios de 7 Unidades de Alimentação e Nutrição Hospitalares localizadas em Sergipe, ofertados a funcionários e acompanhantes de pacientes, nos almoços do mês de novembro de 2017. Para a análise, utilizou-se o método da Avaliação Qualitativa das Preparações do Cardápio – AQPC. De acordo com o método APQC, a maior parte dos cardápios apresentaram ofertas regulares em relação à presença de fruta (42,9%), folhosos (85,7%), monotonia de cores (57,1%), alimentos ricos em enxofre (85,7%) e doces (42,9%). Já os critérios fritura, fritura associada a doces e conservas, apresentaram percentuais de ocorrência variando, predominantemente, entre bom e ótimo. Apesar dos resultados positivos, ofertas inadequadas ou próximas à inadequação foram observadas em algumas unidades, principalmente em relação à monotonia de cores, presença de alimentos ricos em enxofre, baixa oferta de frutas, ocorrência de conservas e doces, devendo estes, serem, objetos de atenção quanto ao planejamento dos cardápios, em função dos possíveis efeitos deletérios ao organismo. Assim, cada Unidade deve buscar ajustar os diferentes aspectos que compõem os cardápios, sendo indispensável a atenção a todas as questões que envolvem uma alimentação saudável.
Joint Use of Genome, Pedigree, and Their Interaction with Environment for Predicting the Performance of Wheat Lines in New Environments
Authors: Howard Réka , Gianola Daniel , Montesinos-López Osval , Juliana Philomin , Singh Ravi , Poland Jesse , Shrestha Sandesh , Pérez-Rodríguez Paulino , Crossa José , Jarquín Diego 
Published: July 2019

Abstract
Genome-enabled prediction plays an essential role in wheat breeding because it has the potential to increase the rate of genetic gain relative to traditional phenotypic and pedigree-based selection. Since the performance of wheat lines is highly influenced by environmental stimuli, it is important to accurately model the environment and its interaction with genetic factors in prediction models. Arguably, multi-environmental best linear unbiased prediction (BLUP) may deliver better prediction performance than single-environment genomic BLUP. We evaluated pedigree and genome-based prediction using 35,403 wheat lines from the Global Wheat Breeding Program of the International Maize and Wheat Improvement Center (CIMMYT). We implemented eight statistical models that included genome-wide molecular marker and pedigree information as prediction inputs in two different validation schemes. All models included main effects, but some considered interactions between the different types of pedigree and genomic covariates via Hadamard products of similarity kernels. Pedigree models always gave better prediction of new lines in observed environments than genome-based models when only main effects were fitted. However, for all traits, the highest predictive abilities were obtained when interactions between pedigree, genomes, and environments were included. When new lines were predicted in unobserved environments, in almost all trait/year combinations, the marker main-effects model was the best. These results provide strong evidence that the different sources of genetic information (molecular markers and pedigree) are not equally useful at different stages of the breeding pipelines, and can be employed differentially to improve the design and prediction of the outcome of future breeding programs.
IMPLEMENTASI GURU DALAM MENDESAIN PROSES PEMBELAJARAN PAI
Authors: Nurhayati Nuy , Fhatulloh Muhammad Ramdlan , Yusup Mahmud 
Published: July 2019

Pembelajaran yang didesain atau direncanakan haruslah efektif dan efisien sehingga tujuan pembelajaran tercapai dan diterima dengan baik oleh peserta didik sehingga tujuan nasional pendidik mampu dicapai dengan baik. Dalam pembelajaran dan pendidikan seiring dengan berkembangnya pendidikan dan sistem pendidikan di Indonesia, seluruh elemen masyarakat, utamanya yang terkait langsung dengan pendidikan dituntut untuk lebih kreatif dan profesional untuk mengembangkan pendidikan. Selain itu, para pelaku pendidikan juga diharapkan sesuai dengan prosedur yang telah ditetapkan bersama sesuai dengan kebutuhan dan tantangan pendidikan.Untuk itulah perlu adanya cara atau metode untuk menjawab tantangan-tantangan yang muncul seiring dengan berkembangnya waktu, maka muncullah cara atau metode yang disebut perencanaan dan desain pembelajaran yang diharapkan akan lebih memudahkan proses belajar mengajar, dan khususnya yang berkaitan dengan pendidikan agama Islam