bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Scenarios of the Application of Electrical Impedance Tomography in Paediatric Intensive Care
Authors: Davies Patrick , Yasin Samra , Gates Simon , Bird David , Silvestre Catarina 
Published: March 2019

AbstractEIT is a radiation-free functional modality that enables bedside imaging and monitoring of lung function and expansion. Clinical interest in this method has been driven by the need for bedside monitoring of the dynamics of the lungs and the effects of ventilatory manoeuvres, including changes in ventilator settings, suctioning, chest drains, positioning and physiotherapy. We aimed to describe the use of Electrical Impedance Tomography (EIT) as a clinical tool in a tertiary Paediatric Intensive Care unit. Children requiring intensive care with a variety of clinical conditions had an electrode belt with 16 electrodes wrapped around the chest, which sequentially applied a small alternating current from each electrode pair. The signal gives information on both real time, regional, global, and relative data. With the correct application, and understanding of the monitor, much clinical information can be gained, with potentially significant patient benefit. We present the clinical use of EIT in six conditions: Asthma, Ventilation weaning and expansion recoil, Sequential Lobar Collapse, Targeted Physiotherapy, Pleural Effusion assessment, and PEEP optimisation. Screenshots and analyses are offered displaying the pragmatic use of this technology. Electrical Impedance Tomography is a clinically useful tool on the Paediatric Intensive Care unit. It allows monitoring of a patient’s respiratory function in ways which are not possible through any other means. An understanding of respiratory physiology will allow use of this information to improve patient outcomes.
Optimal control of the transport of Bose-Einstein condensates with atom chips
Authors: Amri S. , Corgier R. , Sugny D. , Rasel E. M. , Gaaloul N. , Charron E. 
Published: March 2019

AbstractUsing Optimal Control Theory (OCT), we design fast ramps for the controlled transport of Bose-Einstein condensates with atom chips’ magnetic traps. These ramps are engineered in the context of precision atom interferometry experiments and support transport over large distances, typically of the order of 1 mm, i.e. about 1,000 times the size of the atomic clouds, yet with durations not exceeding 200 ms. We show that with such transport durations of the order of the trap period, one can recover the ground state of the final trap at the end of the transport. The performance of the OCT procedure is compared to that of a Shortcut-To-Adiabaticity (STA) protocol and the respective advantages/disadvantages of the OCT treatment over the STA one are discussed.
Source, Spatial Distribution and Pollution Assessment of Pb, Zn, Cu, and Pb, Isotopes in urban soils of Ahvaz City, a semi-arid metropolis in southwest Iran
Authors: Nazarpour Ahad , Watts Michael J. , Madhani Ayoub , Elahi Somayeh 
Published: March 2019

AbstractThis study examined the status of toxic metal contamination of the urban industrial city of Ahvaz in Iran. Two hundred and twenty-seven surface soils from a depth horizon of 0–10 cm were collected from urban areas. In addition, 15 soil samples were collected to recognise the sources of Pb in urban topsoils in Ahvaz city. Mean concentration of Pb, Zn, Cu and As were 181 ± 167, 123 ± 118, 185 ± 167 and 6.9 ± 8.9 mg.kg−1, respectively. Results of inter-element relationship among studied toxic metals revealed that Pb, Zn and Cu may have the same anthropogenic origin, whilst As originated from different sources. The results of pollution index (PI) and Nemerow pollution index (NPI) implied that Pb, Zn, and Cu had a moderate to high level of pollution. The Pb isotopic composition analysis suggested clear anthropogenic origins of Pb including industrial emission, vehicle exhaust and dust storm with the mean contributions of 47%, 15% and 7%, respectively, by a four-end member model.
Alternative sigma factor B (σB) and catalase enzyme contribute to Staphylococcus epidermidis biofilm’s tolerance against physico-chemical disinfection
Authors: Olwal Charles Ochieng’ , Ang’ienda Paul Oyieng’ , Ochiel Daniel Otieno 
Published: March 2019

AbstractStaphylococcus epidermidis is the predominant cause of recalcitrant biofilm-associated infections, which are often highly resistant to antibiotics. Thus, the use of physico-chemical agents for disinfection offers a more effective approach to the control of S. epidermidis biofilm infections. However, the underlying tolerance mechanisms employed by S. epidermidis biofilm against these physico-chemical disinfectants remain largely unknown. The expression of a σB-dependent gene, alkaline shock protein 23 (asp23) and catalase activity by S. epidermidis biofilm and planktonic cells exposed to heat (50 °C), 0.8 M sodium chloride (NaCl), 5 mM sodium hypochlorite (NaOCl) or 50 μM hydrogen peroxide (H2O2) for 60 minutes were compared. Significantly higher asp23 expression levels were observed in biofilms exposed to 50 °C, 5 mM NaOCl or 50 μM H2O2 compared to the corresponding planktonic cells (p < 0.05). Conversely, asp23 expression levels in biofilm and planktonic cells exposed to 0.8 M NaCl were not significantly different (p > 0.05). Further, biofilms exposed to 50 °C, 0.8 M NaCl, 5 mM NaOCl or 50 μM H2O2 exhibited significantly higher catalase activity than the planktonic cells (p < 0.05). These results suggest that activities of σB and catalase may be involved in the tolerance of S. epidermidis biofilm against physico-chemical disinfection.
Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications
Authors: Patel Girijesh Kumar , Khan Mohammad Aslam , Zubair Haseeb , Srivastava Sanjeev Kumar , Khushman Moh’d , Singh Seema , Singh Ajay Pratap 
Published: March 2019

AbstractExosomes have received significant attention for their role in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for exosome isolation. Here we compared the efficacy of four kits from Invitrogen, 101Bio, Wako and iZON along with conventional ultracentrifugation-based method for exosome yield, purity and quality. Cell culture supernatant was used as an abundant source of exosomes, and exosome quantity, size-distribution, zeta-potential, marker-expression and RNA/protein quality were determined. The Invitrogen kit gave the highest yield but the preparation showed broader size-distribution likely due to microvesicle co-precipitation and had the least dispersion stability. Other preparations showed <150 nm size range and good stability. Preparation from iZON column; however, had a broader size-distribution in the lower size range suggestive of some impurities of non-vesicular aggregates. RNA quality from all preparations was comparable; however, proteins from Invitrogen method-based exosomal preparation showed polyethylene glycol (PEG) contamination in mass spectrometry. Chemical impurities from the precipitant could also be the cause of toxicity of Invitrogen method-based exosomal preparation in biological growth measurement assay. Together, these findings should serve as a guide to choose and further optimize exosome isolation methods for their desired downstream applications.
Selective manipulation of the inositol metabolic pathway for induction of salt-tolerance in indica rice variety
Authors: Mukherjee Rajeswari , Mukherjee Abhishek , Bandyopadhyay Subhendu , Mukherjee Sritama , Sengupta Sonali , Ray Sudipta , Majumder Arun Lahiri 
Published: March 2019

AbstractHalophytes are rich sources of salt stress tolerance genes which have often been utilized for introduction of salt-tolerance character in salt-sensitive plants. In the present study, we overexpressed PcINO1 and PcIMT1 gene(s), earlier characterized in this laboratory from wild halophytic rice Porteresia coarctata, into IR64 indica rice either singly or in combination and assessed their role in conferring salt-tolerance. Homozygous T3/T4 transgenic plants revealed that PcINO1 transformed transgenic rice lines exhibit significantly higher tolerance upto 200 mM or higher salt concentration with negligible compromise in their growth or other physiological parameters compared to the untransformed system grown without stress. The PcIMT1-lines or the double transgenic lines (DC1) having PcINO1 and PcIMT1 introgressed together, were less efficient in such respect. Comparison of inositol and/or pinitol pool in three types of transgenic plants suggests that plants whose inositol production remains uninterrupted under stress by the functional PcINO1 protein, showed normal growth as in the wild-type plants without stress. It is conceivable that inositol itself acts as a stress-ameliorator and/or as a switch for a number of other pathways important for imparting salt-tolerance. Such selective manipulation of the inositol metabolic pathway may be one of the ways to combat salt stress in plants.
Diffraction-less propagation beyond the sub-wavelength regime: a new type of nanophotonic waveguide
Authors: Alonso-Ramos Carlos , Le Roux Xavier , Zhang Jianhao , Benedikovic Daniel , Vakarin Vladyslav , Durán-Valdeiglesias Elena , Oser Dorian , Pérez-Galacho Diego , Mazeas Florent , Labonté Laurent , Tanzilli Sébastien , Cassan Éric , Marris-Morini Delphine , Cheben Pavel , Vivien Laurent 
Published: March 2019

AbstractSub-wavelength grating (SWG) metamaterials have garnered a great interest for their singular capability to shape the propagation of light. However, practical SWG implementations are limited by fabrication constraints, such as minimum feature size. Here, we present a new nanophotonic waveguide grating concept that exploits phase-matching engineering to suppress diffraction effects for a period three times larger than those with SWG approaches. This long-period grating not only facilitates fabrication, but also enables a new diffraction-less regime with additional degrees of freedom to control light propagation. More specifically, the proposed phase-matching engineering enables selective diffraction suppression, providing new tools to shape propagation in the grating. We harness this flexible diffraction control to yield single-mode propagation in, otherwise, highly multimode waveguides, and to implement Bragg filters that combine highly-diffractive and diffraction-less regions to dramatically increase light rejection. Capitalizing on this new concept, we experimentally demonstrate a Si membrane Bragg filter with record rejection value exceeding 60 dB. These results demonstrate the potential of the proposed long-period grating for the engineering of diffraction in nanophotonic waveguides and pave the way for the development of a new generation of high-performance Si photonics devices.
Massive benthic litter funnelled to deep sea by flash-flood generated hyperpycnal flows
Authors: Pierdomenico Martina , Casalbore Daniele , Chiocci Francesco Latino 
Published: March 2019

AbstractMarine litter is an emerging environmental threat affecting all world’s oceans including the deep seafloor, where the extent of the phenomenon is still largely unknown. We report the spatial patterns of macro-litter distribution within the Messina Strait’s channels (Central Mediterranean), focusing on the transfer mechanisms responsible for its emplacement, a key information to better understand litter distribution. Litter is patchy but pervasive on all surveyed channels, reaching densities up to ~200 items/10 m, the highest reported for the deep sea until now. Litter is often arranged in large accumulations formed by hundreds of land-sourced items, mixed to vegetal and coarse-grained debris, indicating an emplacement from sedimentary gravity flows. Such impressive amount of litter can be explained by the superposition of a very efficient source-to-sink sedimentary transport and a strong urbanization of the coastal area. These findings point out that macro-benthic litter pollution is a major, often overlooked, threat for deep-sea ecosystems. Further explorations are thus required in similar marine settings to fully understand the magnitude of the problem, since they may represent the largest litter hotspots in the deep-sea.
No signal of deleterious mutation accumulation in conserved gene sequences of extant asexual hexapods
Authors: Brandt Alexander , Bast Jens , Scheu Stefan , Meusemann Karen , Donath Alexander , Schütte Kai , Machida Ryuichiro , Kraaijeveld Ken 
Published: March 2019

AbstractLoss of sex and recombination is generally assumed to impede the effectiveness of purifying selection and to result in the accumulation of slightly deleterious mutations. Empirical evidence for this has come from several studies investigating mutational load in a small number of individual genes. However, recent whole transcriptome based studies have yielded inconsistent results, hence questioning the validity of the assumption of mutational meltdown in asexual populations. Here, we study the effectiveness of purifying selection in eight asexual hexapod lineages and their sexual relatives, as present in the 1 K Insect Transcriptome Evolution (1KITE) project, covering eight hexapod groups. We analyse the accumulation of slightly deleterious nonsynonymous and synonymous point mutations in 99 single copy orthologue protein-coding loci shared among the investigated taxa. While accumulation rates of nonsynonymous mutations differed between genes and hexapod groups, we found no effect of reproductive mode on the effectiveness of purifying selection acting at nonsynonymous and synonymous sites. Although the setup of this study does not fully rule out nondetection of subtle effects, our data does not support the established consensus of asexual lineages undergoing ‘mutational meltdown’.
Analysis of α-dicarbonyl compounds and volatiles formed in Maillard reaction model systems
Authors: Cha Jiyoon , Debnath Trishna , Lee Kwang-Geun 
Published: March 2019

AbstractIn this study, production of three α-dicarbonyl compounds (α-DCs) including glyoxal (GO), methylglyoxal (MGO), and diacetyl (DA) as well as volatile flavor compounds was analyzed using Maillard reaction (MR) model systems. A total of 16 model systems were assembled using four amino acids and four reducing sugars, and reactions were performed at 160 °C and pH 9. Determination of α-DCs was conducted using a gas chromatography/nitrogen phosphorous detector (GC-NPD) after derivatization and liquid-liquid extraction. α-DC levels in MR model systems were 5.92 to 39.10 μg/mL of GO, 3.66 to 151.88 μg/ml of MGO, and 1.10 to 6.12 μg/mL of DA. The highest concentration of total α-DCs was found in the fructose-threonine model system and the lowest concentration in the lactose-cysteine model system. Volatile flavor compounds were analyzed using solid-phase micro-extraction (SPME) followed by GC-mass spectrometry (GC-MS). Different volatile flavor compound profiles were identified in the different MR model systems. Higher concentrations of α-DCs and volatile flavor compounds were observed in monosaccharide-amino acid MR model systems compared with disaccharide-amino acid model systems.
Preventing lung pathology and mortality in rabbit Staphylococcus aureus pneumonia models with cytotoxin-neutralizing monoclonal IgGs penetrating the epithelial lining fluid
Authors: Stulik Lukas , Rouha Harald , Labrousse Delphine , Visram Zehra Claire , Badarau Adriana , Maierhofer Barbara , Groß Karin , Weber Susanne , Kramarić Miroslava Dominis , Glojnarić Ines , Nagy Gábor , Croisier Delphine , Nagy Eszter 
Published: March 2019

AbstractStaphylococcus aureus pneumonia is associated with high mortality irrespective of antibiotic susceptibility. Both MRSA and MSSA strains produce powerful cytotoxins: alpha-hemolysin(Hla) and up to five leukocidins – LukSF-PV, HlgAB, HlgCB, LukED and LukGH (LukAB) – to evade host innate defense mechanisms. Neutralizing cytotoxins has been shown to provide survival benefit in rabbit S. aureus pneumonia models. We studied the mechanisms of protection of ASN100, a combination of two human monoclonal antibodies (mAbs), ASN-1 and ASN-2, that together neutralize Hla and the five leukocidins, in rabbit MRSA and MSSA pneumonia models. Upon prophylactic passive immunization, ASN100 displayed dose-dependent increase in survival and was fully protective against all S. aureus strains tested at 5 or 20 mg/kg doses. Macroscopic and microscopic lung pathology, edema rate, and bacterial burden were evaluated 12 hours post infection and reduced by ASN100. Pharmacokinetic analysis of ASN100 in bronchoalveolar-lavage fluid from uninfected animals detected efficient penetration to lung epithelial lining fluid reaching peak levels between 24 and 48 hours post dosing that were comparable to the mAb concentration measured in serum. These data confirm that the ASN100 mAbs neutralize the powerful cytotoxins of S. aureus in the lung and prevent damage to the mucosal barrier and innate immune cells.
Heterogeneous drug tissue binding in brain regions of rats, Alzheimer’s patients and controls: impact on translational drug development
Authors: Gustafsson Sofia , Sehlin Dag , Lampa Erik , Hammarlund-Udenaes Margareta , Loryan Irena 
Published: March 2019

AbstractFor preclinical and clinical assessment of therapeutically relevant unbound, free, brain concentrations, the pharmacokinetic parameter fraction of unbound drug in brain (fu,brain) is commonly used to compensate total drug concentrations for nonspecific brain tissue binding (BTB). As, homogenous BTB is assumed between species and in health and disease, rat BTB is routinely used. The impact of Alzheimer’s disease (AD) on drug BTB in brain regions of interest (ROI), i.e., fu,brain,ROI, is yet unclear. This study for the first time provides insight into regional drug BTB and the validity of employing rat fu,brain,ROI as a surrogate of human BTB, by investigating five marketed drugs in post-mortem tissue from AD patients (n = 6) and age-matched controls (n = 6). Heterogeneous drug BTB was observed in all within group comparisons independent of disease and species. The findings oppose the assumption of uniform BTB, highlighting the need of case-by-case evaluation of fu,brain,ROI in translational CNS research.
Computer aided protein engineering to enhance the thermo-stability of CXCR1- T4 lysozyme complex
Authors: Wang Yang , Park Jae-Hyun , Lupala Cecylia Severin , Yun Ji-Hye , Jin Zeyu , Huang Lanqing , Li Xuanxuan , Tang Leihan , Lee Weontae , Liu Haiguang 
Published: March 2019

AbstractCXCR1, a member in G-protein coupled receptor (GPCR) family, binds to chemokine interleukin-8 (IL-8) specifically and transduces signals to mediate immune and inflammatory responses. Despite the importance of CXCR1, high-resolution structure determination is hindered by the challenges in crystallization. It has been shown that properly designed mutants with enhanced thermostability, together with fusion partner proteins, can be useful to form crystals for GPCR proteins. In this study,in silicoprotein design was carried out by using homology modeling and molecular dynamics simulations. To validate the computational modeling results, the thermostability of several mutants and the wild type were measured experimentally. Both computational results and experimental data suggest that the mutant L126W has a significant improvement in the thermostability. This study demonstrated thatin silicodesign can guide protein engineering and potentially facilitate protein crystallography research.
Comparison of recombinant and urinary follicle-stimulating hormones over 2000 gonadotropin-releasing hormone antagonist cycles: a retrospective study
Authors: Pan Wei , Tu Haiting , Jin Lei , Hu Cheng , Xiong Jianwu , Pan Wulin , Yu Dongyang , Wang Renjie , Li Yuehan , Huang Weiming , Liao ShuJie 
Published: March 2019

AbstractThe objective of this paper was to compare the effect of recombinant follicle-stimulating hormone (rFSH) and urinary follicle-stimulating hormone (uFSH) on pregnancy rates and live birth rates with the gonadotropin-releasing hormone (GnRH) antagonist protocol in China. This retrospective study was conducted from January 2014 through August 2017. Patients treated with uFSH had significantly higher levels of luteinizing hormone (3.79 mIU/ml vs. 3.09 mIU/ml) and progesterone (0.93 ng/ml vs. 1.16 ng/ml) on the day of human chorionic gonadotropin (HCG) administration, and they also had higher pregnancy rates (24.19% vs. 22.86%). There was no significant difference in the rate of live births. In the logistic regression results of the rFSH group, the pregnancy rate was positively correlated with the level of luteinizing hormone, with an odds ratio (OR) of 1.09 (95% confidence interval [CI]: 1.00–1.18; P = 0.048). In the uFSH group, the pregnancy rate was negatively correlated with the progesterone level on the day of HCG administration, with an OR of 0.47 (95% CI: 0.27–0.77; P = 0.004). Our research concluded that uFSH performed better than rFSH in terms of pregnancy rates when it was associated with the GnRH antagonist protocol. Meanwhile, no significant differences in the rate of live births were observed between the two groups.
Streptolysin S induces mitochondrial damage and macrophage death through inhibiting degradation of glycogen synthase kinase-3β in Streptococcus pyogenes infection
Authors: Tsao Nina , Kuo Chih-Feng , Cheng Miao-Hui , Lin Wei-Chen , Lin Chiou-Feng , Lin Yee-Shin 
Published: March 2019

AbstractGroup A Streptococcus (GAS) infection is associated with a variety of human diseases. Previous studies indicate GAS infection leads to RAW264.7 cell death, but the mechanism is unclear. Here, analyzing the timing of reactive oxygen species (ROS) production and using mitochondrial ROS scavenger, we found the wild type GAS-induced RAW264.7 cell death was associated with mitochondrial ROS. The wild type GAS infection could activate glycogen synthase kinase-3β (GSK-3β). Inhibition of GSK-3β activity by lithium chloride or decreasing GSK-3β expression by lentivirus-mediated short hairpin RNA for GSK-3β could not only decrease the wild type GAS-induced mitochondrial ROS generation, mitochondria damage and cell death, but also reduced GAS intracellular replication. Streptolysin S (SLS), a GAS toxin, played the important role on GAS-induced macrophage death. Compared to the wild type GAS with its isogenic sagB mutant (SLS mutant)-infected macrophages, we found sagB mutant infection caused less mitochondrial ROS generation and cell death than those of the wild type GAS-infected ones. Furthermore, the sagB mutant, but not the wild type or the sagB-complementary mutant, could induce GSK-3β degradation via a proteasome-dependent pathway. These results suggest that a new mechanism of SLS-induced macrophage death was through inhibiting GSK-3β degradation and further enhancing mitochondrial damage.