bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low-flow CO2 removal in combination with renal replacement therapy effectively reduces ventilation requirements in hypercapnic patients: a pilot study
Authors: Nentwich Jens , Wichmann Dominic , Kluge Stefan , Lindau Simone , Mutlak Haitham , John Stefan 
Published: January 2019

Abstract
Background
Lung-protective strategies are the cornerstone of mechanical ventilation in critically ill patients with both ARDS and other disorders. Extracorporeal CO2 removal (ECCO2R) may enhance lung protection by allowing even further reductions in tidal volumes and is effective in low-flow settings commonly used for renal replacement therapy. In this study, we describe for the first time the effects of a labeled and certified system combining ECCO2R and renal replacement therapy on pulmonary stress and strain in hypercapnic patients with renal failure.

Methods
Twenty patients were treated with the combined system which incorporates a membrane lung (0.32 m2) in a conventional renal replacement circuit. After changes in blood gases under ECCO2R were recorded, baseline hypercapnia was reestablished and the impact on ventilation parameters such as tidal volume and driving pressure was recorded.

Results
The system delivered ECCO2R at rate of 43.4 ± 14.1 ml/min, PaCO2 decreased from 68.3 ± 11.8 to 61.8 ± 11.5 mmHg (p < 0.05) and pH increased from 7.18 ± 0.09 to 7.22 ± 0.08 (p < 0.05). There was a significant reduction in ventilation requirements with a decrease in tidal volume from 6.2 ± 0.9 to 5.4 ± 1.1 ml/kg PBW (p < 0.05) corresponding to a decrease in plateau pressure from 30.6 ± 4.6 to 27.7 ± 4.1 cmH2O (p < 0.05) and a decrease in driving pressure from 18.3 ± 4.3 to 15.6 ± 3.9 cmH2O (p < 0.05), indicating reduced pulmonary stress and strain. No complications related to the procedure were observed.

Conclusions
The investigated low-flow ECCO2R and renal replacement system can ameliorate respiratory acidosis and decrease ventilation requirements in hypercapnic patients with concomitant renal failure.
Trial registration NCT02590575, registered 10/23/2015.
Akt and SGK protein kinases are required for efficient feeding by macropinocytosis
Authors: Williams Thomas D. , Peak-Chew Sew-Yeu , Paschke Peggy , Kay Robert R. 
Published: January 2019

ABSTRACT
Macropinocytosis is an actin-driven process of large-scale and non-specific fluid uptake used for feeding by some cancer cells and the macropinocytosis model organism Dictyostelium discoideum. In Dictyostelium, macropinocytic cups are organized by ‘macropinocytic patches’ in the plasma membrane. These contain activated Ras, Rac and phospholipid PIP3, and direct actin polymerization to their periphery. We show that a Dictyostelium Akt (PkbA) and an SGK (PkbR1) protein kinase act downstream of PIP3 and, together, are nearly essential for fluid uptake. This pathway enables the formation of larger macropinocytic patches and macropinosomes, thereby dramatically increasing fluid uptake. Through phosphoproteomics, we identify a RhoGAP, GacG, as a PkbA and PkbR1 target, and show that it is required for efficient macropinocytosis and expansion of macropinocytic patches. The function of Akt and SGK in cell feeding through control of macropinosome size has implications for cancer cell biology.
ЭКСПЕРИМЕНТАЛЬНОЕ ЗАМЕЩЕНИЕ КОСТНЫХ ДЕФЕКТОВ МОЗГОВОГО ЧЕРЕПА С ИСПОЛЬЗОВАНИЕМ ТОНКОПРОФИЛЬНОГО НИКЕЛИДА ТИТАНА
Authors: Radkevich Andrey Anatolevich , Gyunter Viktor Eduardovich , Kasparov Eduard Vilyamovich , Pulikov Anatoly Stepanovich , Peretyatko Olga Viktorovna , Mamedov Rasim Khaligovich , Sinyuk Ilya Vasilevich , Kaunietis Sofya Maksimovna , Podgorny Vasily Yuryevich , Zhuravleva Tatyana Borisovna 
Published: January 2019

Цель. Разработка новой медицинской технологии хирургического устранения дефектов костей мозгового черепа с использованием материалов с памятью формы.Методика. Экспериментальное замещение костных структур мозгового черепа выполнено на 69 беспородных кроликах в возрасте 1–1,5 лет. Дефекты создавали искусственно путем симметричного иссечения костной ткани в теменных и височных областях размером 15,0×15,0 мм без нарушения целостности твердой мозговой оболочки. Для устранения полученных дефектов применяли тонкопрофильный сверхэластичный сетчатый никелид титана толщиной 40, 60 и 90 мкм помещенный в один, два и четыре слоя. В целях морфологического изучения материал забирали на 7, 14, 21, 28 и 35-е сут. В каждом варианте выполнено 3 серии опытов (всего 135 с учетом кратности забора материала для гистологических исследований). Контролем служили 3 аналогично образованных изъяна, оставленных без замещения.Результаты. Установлено, что оптимальная структура регенерата получена в результате применения в качестве имплантационного материала четырехслойной сетчатой структуры, изготовленной из нити толщиной 40 мкм. В толще наружных слоев получена органотипичная пластинчатая и губчатая костная ткань, в толще внутренних слоев – плотная полуоформленная соединительная ткань. Образованный в этих случаях единый с имплантационным материалом регенерат дает возможность в полной мере восстанавливать анатомические и функциональные особенности костных структур мозгового черепа, твердой мозговой оболочки и головного мозга. Изучение процессов остеогенеза в зависимости от толщины нитей, из которых был изготовлен имплантационный материал, не выявил существенных отличий. Полученные результаты позволяет рекомендовать внедрение разработанной технологии в широкую клиническую практику.
VALIDASI METODE ANALISIS POLISIKLIK AROMATIK HIDROKARBON (PAH) DALAM SEDIMEN DI SEKITAR PANTAI MAKASSAR
Authors: Syahrir Muhammad , Aprilita Nurul Hidayat , Nuryono Nuryono 
Published: January 2019

Validation of analysis method of PAH have been done in marine sediment of Macassar Coast by Gas Chromatography (GC-FID). Validation of method include: limit of detection (LoD), limit of quantification (LoQ) and linearity range. The results of validation method of 7 types PAHs (Naphtalene, Acenaphtene, Penanthrene, Fluoranthene, Pyrene, Benzo (a) Anthracene and perylene) give a linearity range between 0.5 to 20 ppm with an average linearity approaches a value of 1. LoD for Naphtalene; Acenaphthene; Penanthrene; Fluoranthene; Pyrene; Benzo (a) Anthracene; and Perylene are 3.541; 0.208; 1,736; 1.737; 1,738; 12.124; and 2,128 ppm, respectively. LoQ for Naphtalene; Acenaphthene; Penanthrene; Fluoranthene; Pyrene; Benzo (a) Anthracene; and Perylene are 11,803; 0.695; 5.786; 5.786; 5.788; 5.794; 7.0796 and 7.092 ppm, respectively. Limit of detection (LoD), limit of quantification (LoQ), linearity and linear range can be used to determine the concentration of PAH in sediment samples.Keywords: Validation Method, Concentration, PAH, Sediments
ADSORPSI ION MN(II) PADA ZEOLIT DARI ABU DASAR BATUBARA TERMODIFIKASI DITIZON
Authors: Putra Riandy , Khamidinal Khamidinal , Krisdiyanto Didik , Nugraha Irwan 
Published: January 2019

Adsorption characteristic of Mn(II) on zeolite synthesized from bottom ash with alkaline hydrothermal conditions loaded with dithizone have been examined. The parameters studied includes effect of pH, contact time, initial concentrations and temperature of Mn(II) by batch experiments. The raw and modified samples were characterized by XRF, XRD, FT-IR and SAA. The results of XRD and FTIR showed that hydrothermal product consists of a mixture of zeolite (zeolite Y, zeolite Na-P and zeolite X) and sodalite crystals. Hydrothermal product showed specific IR absorption zeolite at wavenumber 300-1250 cm-1 and zeolite modified dithizone showed absorption -NH and –SH at wavenumber 1496,76 and 2461,17 cm-1. The results SAA showed that with addition dithizone lowering the specific surface area of zeolite from 160,262 m²/g to 69,609 m²/g. Results of study show that adsorption of Mn(II) reaches its maximum values at pH 6 for zeolite and pH 8 for zeolite modified dithizone, respectively. The adsorption kinetics based on the pseudo-second-order rate equation with adsorption rate constants for zeolite synthesis -0,014 g/mg.min-1and for the zeolite modified dithizone 0,0204 g/mg.min-1. Based on the Langmuir equation, the maximum adsorption capacity (qmax) synthetic zeolite of 14,493 mg/g and zeolite modified dhitizone 15,873 mg/g. Termodynamic parameter indicated thatadsorption using two adsorbents takes place spontaneously at a lower temperature.Keywords: Bottom ash, zeolite, hydrothermal, dithizone, adsorption
PURIFIKASI PARSIAL DAN KARAKTERISASI ß-GALAKTOSIDASE Lactobacillus plantarum B123 INDIGENOS DAN HIDROLISIS LAKTOSA UNTUK PRODUKSI SUSU ULTRA HIGH TEMPERATURE RENDAH LAKTOSA
Authors: Khusniati Tatik , Mariyani Neny , Lioe Hanifah Nuryani , Faridah Didah Nur , Choliq Abdul , Sulistiani Sulistiani 
Published: January 2019

β-Galactosidase is enzyme which hidrolyze lactose to glucose and galactose. This enzyme is used in production low lactose milk for consumption human which have lactose intolerance. Partial purification of β-galactosidase is important to be conducted to increase β-galactosidase activity in order to its hydrolysis potency on UHT milk lactose increased.This research was aimed to production by partially purification and characterization indigenous β-galactosidase from Lactobacillus plantarum B123, and lactose hydrolysis for production low lactose UHT milk. Partially purification were precipitation following dialysis. Characterization included optimazion and stabilization of enzyme, while lactose hydrolisis for production low lactose UHT milk was detected by enzymatic GOD-POD kit. The results showed that production of β-galactosidase by using partial purification increased from 21.51 ± 0.23 U/mL (crude) to 106.34 ± 0.56 U/mL (dialysis). The optimum crude β-galactosidase activity was reached in precipitation by using 60 % ammonium sulphate. The purity of crude β-galactosidase increased 3.71 times after precipitation, and 14.28 times after dialysis. Characterization of β-galactosidase showed that optimum activities of crude and dialyzed β-galactosidase were at pH 6.5 and 50 oC, respectively. Stability of crude β-galactosidase incubated for 1 h were at pH: 5.0-8.5 and 25-50 °C. Specific activity of crude β-galactosidase was 15.05 U/mg protein, while that dialyzed β-galactosidase was 109.58 U/mg protein. Lactose hidrolysis to produce low lactose UHT milk showed that glucose concentration increased with the increase of hidrolysis time. Time needed to hidrolyze lactose 50 % with 4.8 U/mL β-galactosidase at 50°C was 6.08 h. In conclusion that indigenous β-galactosidase from Lactobacillus plantarum B123 purified partially can be used as lactose hidrolyzer in production of low lactose UHT milk.Key words : b-galactosidase, indigenous Lactobacillus plantarum B123, purification, lactose hidrolysis, UHT Milk
Mental health service users’ perceptions of data sharing and data protection: a short qualitative report
Authors: Satinsky Emily , Driessens Corine , Crepaz-Keay David , Kousoulis Antonis A. 
Published: January 2019

BackgroundTo improve health services, social, economic and health data should be shared and linked to create a full narrative of lived experience. Mental health data sharing is often considered a particularly sensitive area.ObjectiveTo assess mental health service users’ perceptions regarding the current practice of administrative data-driven research.MethodWe conducted a focus group using case study scenarios. Themes and subthemes were analysed using qualitative methods.ResultsParticipants were generally happy for data owners to share their health, social and economic data if the purpose was transparent and if the information would inform and improve health policy and practice. Participants were less keen on sharing data through digital applications.ConclusionThis case study informs a data linkage study protocol. Research teams and database owners should strive to educate service users on data protection and create dissent opportunities.
Regime de colaboração na política de formação continuada para professores alfabetizadores: uma tentativa da garantia do direito à educação
Authors: Pereira de Souza Elaine Constant 
Published: January 2019

O artigo analisa a implementação do Pacto Nacional pela Alfabetização na Idade Certa e o Regime de Colaboração presente nessa proposta. Os registros escritos de dois Grupos de Trabalhos (GTs) auxiliaram na compreensão das apropriações sobre o conceito por municípios. O estudo mostrou que as redes públicas do estado do Rio de Janeiro se mostravam inseguras para lidar com o tema, pois não se sentiam autorizadas para se posicionar sobre a política educacional. Mas, no decorrer do desenvolvimento do Programa, houve empoderamento das “vozes” docentes e maior articulação local, em especial, com a Universidade pública. Concluiu-se que as políticas de formação se mostram como desafios, pois tanto as redes públicas quanto para a Universidade, precisaram lidar com a cultura escolar e as implementações de políticas educacionais.
Palavras-chave: política educacional, formação de professores, Regime de Colaboração
Cell death indicators in children under exposure to excessive intake of chlorine compounds with drinking water
Authors: Dianova D. , Zaitseva N. , Dolgikh O. , Krivtsov A. 
Published: January 2019

Objective. The study of the immune status features including cell death markers in children consuming drinking water with excessive content of chlorine compounds. Materials and methods. Including the control group, 498 pre-school children were surveyed. The control group comprised 212 children living on territories where circulating groundwater is used as a source of domestic water supply. The study group consisted of 286 children living on territories with sources of domestic water supply presented by surface water of low quality due to microbiological indicators, which calls for the use of chlorine-containing agents for water disinfection. To conduct the study the chemical-analytical, immunological and statistical methods were used. Results. It was revealed that in the blood of children consuming hyperclorinated water, the concentration of chloroform in a range of 0 mg/dm3 - 0.2300 mg / dm3 has been identified, meanwhile in the biological medium of the control group children, the chloroform has not been detected. Using flow cytometry the statistically significant (р = 0.001) decrease (up to 1.7 fold) of Annexin V-FITC +PI- -cells amount (early apoptosis) and up to 2.4 fold increase (р = 0.001) of Annexin V-FITC+PI+ -cells content (late apoptosis / necrosis) was found in those children, who were exposed to the excessive content of chloroform with drinking water, relative to the values fixed in non-exposed children. Conclusions. It was demonstrated that under the increased chlorine compounds concentrations in blood the probable mechanism of apoptotic effect was related to the enzyme systems activation, associated with glutathione peroxidase, glutathione transferase, superoxide dismutase. The reference concentrations for chlorine-containing compounds in blood were proved by the criteria of IL6 and CD4+ -, CD8+ -, CD19+ -, CD25+ - lymphocytes content and their acceptable concentration range in blood (0.00001 mg / dm3 - 0.0181 mg / dm3) was defined.
Acclimation limits of Fucus evanescens along the salinity gradient of the southwestern Baltic Sea
Authors: Romoth Katharina , Nowak Petra , Kempke Daniela , Dietrich Anna , Porsche Christian , Schubert Hendrik 
Published: January 2019

Abstract
Over recent decades, the neophyte Fucus evanescens has extended eastwards along the salinity gradient within the Baltic Sea, indicating gradual adaptation to low salinity conditions. To find out whether F. evanescens can migrate further into the Baltic Sea and potentially become a competitor to the native F. vesiculosus, the acclimation potentials of different F. evanescens and F. vesiculosus populations were investigated with respect to habitat salinity. For both species, pigmentation, water content, and photosynthetic rate were measured under laboratory and field conditions. The instantaneous measurement data and incubation experiment did not show clear differences in the measured photosynthetic parameters between different salinity levels (6–20), or between species. Maximum likelihood phylogenetic analyses of the nuclear marker PDI (a putative protein disulfide isomerase) separated F. vesiculosus and F. evanescens into well-defined groups supporting the hypothesis that the two very similar species do not represent different morphotypes of the same species/gene pool. These findings indicate that – at least for the vegetative stage of F. evanescens – salinity may not be a limiting factor for a further spread into the Baltic Sea.
Mass transport and yield during spinning of lignin-cellulose carbon fiber precursors
Authors: Bengtsson Jenny , Jedvert Kerstin , Hedlund Artur , Köhnke Tobias , Theliander Hans 
Published: January 2019

Abstract
Lignin, a substance considered as a residue in biomass and ethanol production, has been identified as a renewable resource suitable for making inexpensive carbon fibers (CFs), which would widen the range of possible applications for light-weight CFs reinforced composites. Wet spinning of lignin-cellulose ionic liquid solutions is a promising method for producing lignin-based CFs precursors. However, wet-spinning solutions containing lignin pose technical challenges that have to be solved to enable industrialization. One of these issues is that a part of the lignin leaches into the coagulation liquid, which reduces yield and might complicate solvent recovery. In this work, the mass transport during coagulation is studied in depth using a model system and trends are confirmed with spinning trials. It was discovered that during coagulation, efflux of ionic liquid is not hindered by lignin concentration in solution and the formed cellulose network will enclose soluble lignin. Consequently, a high total concentration of lignin and cellulose in solution is advantageous to maximize yield. This work provides a fundamental understanding on mass transport during coagulation of lignin-cellulose solutions, crucial information when designing new solution-based fiber forming processes.
Resistance Training Performed to Failure or Not to Failure Results in Similar Total Volume, but With Different Fatigue and Discomfort Levels
Authors: Santos Wanderson Divino Nilo dos , Vieira Carlos A. , Bottaro Martim , Nunes Vitória A. , Ramirez-Campillo Rodrigo , Steele James , Fisher James P. , Gentil Paulo 
Published: January 2019

Abstract
Santos, WDNd, Vieira, CA, Bottaro, M, Nunes, VA, Ramirez-Campillo, R, Steele, J, Fisher, JP, and Gentil, P. Resistance training performed to failure or not to failure results in similar total volume, but with different fatigue and discomfort levels. J Strength Cond Res 35(5): 1372–1379, 2021—The purpose of this study was to compare the acute response to 4 sets of high velocity parallel squats performed to momentary failure (MF) or not to momentary failure (NF). Twelve women (24.93 ± 5.04 years) performed MF and NF protocols, in a randomized order with 2–3 interday rest. The protocol involved 4 sets of parallel squats executed at high velocity at 10RM load, with 2 minutes of rest interval between sets. During the NF protocol, the sets were interrupted when the subject lost more than 20% of mean propulsive velocity. The analysis involved the number of repetitions performed per set, total number of repetitions, movement velocity loss, power output loss, rating of perceived exertion (RPE), rating of perceived discomfort (RPD), and session rating of perceived exertion (sRPE). Compared with NF, MF resulted in a higher number of repetitions in the first set (11.58 ± 1.83 vs. 7.58 ± 1.72, p < 0.05), but a lower in the last set (3.58 ± 1.08 vs. 5.41 ± 1.08, p < 0.05). Total number of repetitions was similar between the protocols (MF 26.25 ± 3.47 vs. NF 24.5 ± 3.65, p > 0.05). In both protocols, there were significant decreases in maximum and mean movement velocity loss and power output loss, but higher decreases were observed in MF than NF (p < 0.05). Values for RPE, sRPE, and RPD were higher during MF than NF (p < 0.05). Controlling the movement velocity in NF protocol enabled performance of a similar total volume of repetitions with lower movement velocity and power output losses, RPE, sRPE, and RPD than during an MF protocol.
Neural Mechanisms of Emotion Regulation Moderate the Predictive Value of Affective and Value-Related Brain Responses to Persuasive Messages
Authors: Doré Bruce P. , Tompson Steven H. , O'Donnell Matthew B. , An Lawrence C. , Strecher Victor , Falk Emily B. 
Published: January 2019

Emotionally evocative messages can be an effective way to change behavior, but the neural pathways that translate messages into effects on individuals and populations are not fully understood. We used a human functional neuroimaging approach to ask how affect-, value-, and regulation-related brain systems interact to predict effects of graphic anti-smoking messages for individual smokers (both males and females) and within a population-level messaging campaign. Results indicated that increased activity in the amygdala, a region involved in affective reactivity, predicted both personal quit intentions and population-level information-seeking and this was mediated by activity in ventromedial prefrontal cortex (vmPFC), a region involved in computing an integrative value signal. Further, the predictive value of these regions was moderated by expression of a meta-analytically defined brain pattern indexing emotion regulation. That is, amygdala and vmPFC activity strongly tracked with population behavior only when participants showed low recruitment of this brain pattern, which consists of regions involved in goal-driven regulation of affective responses. Overall, these findings suggest that affective and value-related brain responses can predict the success of persuasive messages and that neural mechanisms of emotion regulation can shape these responses, moderating the extent to which they track with population-level message impact.SIGNIFICANCE STATEMENTPeople and organizations often appeal to our emotions to persuade us, but how these appeals engage the brain to drive behavior is not fully understood. We present an fMRI-based model that integrates affect-, control-, and value-related brain responses to predict the impact of graphic anti-smoking stimuli within a small group of smokers and a larger-scale public messaging campaign. This model indicated that amygdala activity predicted the impact of the anti-smoking messages, but that this relationship was mediated by ventromedial prefrontal cortex and moderated by expression of a distributed brain pattern associated with regulating emotion. These results suggest that neural mechanisms of emotion regulation can shape the extent to which affect and value-related brain responses track with population behavioral effects.
Socialinis darbas Lietuvoje: raidos, praktikos ir akademinis aspektai
Authors: Bagdonas Albinas 
Published: January 2019

[full article and abstract in Lithuanian; abstract in English]
Several years ago the reaction of an ordinary Lithuanian citizen, politician or even a professional (teacher, medical doctor, psychologist) to such word combinations a social work or social worker was not only awkward but often negative: What a strange word combination! Is it necessary to make references to work when naming a profession? Maybe such notions of recent past as party work, party worker or trade union worker accounted for the negative attitude towards the said word combinations. Almost 10 years passed, and the concepts, such as social work or social worker became common. They no longer jar on nobody’s ears. However the following questions are still topical: What is social work? What do social workers do? Is it science or art? The majority of sciences have developed from philosophy. They have scientific names, usually of Latin or Greek origin. The beginning of social work is associated with charity or philanthropic activities.