bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti–PD-L1 Treatment Results in Functional Remodeling of the Macrophage Compartment
Authors: Xiong Huizhong , Mittman Stephanie , Rodriguez Ryan , Moskalenko Marina , Pacheco-Sanchez Patricia , Yang Yagai , Nickles Dorothee , Cubas Rafael 
Published: January 2019

Abstract

Checkpoint inhibitors like anti-PD1/PD-L1 have demonstrated significant therapeutic efficacy in a subset of patients partly through reinvigoration of CD8 T cells. However, their impact on myeloid cells remains largely unknown. Here, we report that anti–PD-L1 treatment favorably impacts the phenotype and function of tumor macrophages by polarizing the macrophage compartment toward a more proinflammatory phenotype. This phenotype was characterized by a decrease in Arginase-I (ARG1) expression and an increase in iNOS, MHCII, and CD40 expression. Whole-transcriptome profiling further confirmed extensive polarization of both tumor monocytes and macrophages from a suppressive to a proinflammatory, immunostimulatory phenotype. This polarization was driven mainly through IFNγ and was associated with enhanced T-cell activity. Transfer of monocytes into anti–PD-L1–treated tumor-bearing mice led to macrophage differentiation into a more proinflammatory phenotype, with an increase in CD8 T cells expressing granzyme-B and an increase in the CD8/Treg ratio compared with control-treated mice. Although in responsive tumor models, anti–PD-L1 treatment remodeled the macrophage compartment with beneficial effects on T cells, both macrophage reprogramming and depletion were needed to maximize anti–PD-L1 responses in a tumor immune contexture with high macrophage burden. Our results demonstrate that anti–PD-L1 treatment can favorably remodel the macrophage compartment in responsive tumor models toward a more proinflammatory phenotype, mainly through increased IFNγ levels. They also suggest that directly targeting these cells with reprogramming and depleting agents may further augment the breadth and depth of response to anti–PD-L1 treatment in less responsive or more macrophage-dense tumor microenvironments.

Significance:
This work demonstrates that increased IFNγ signaling following anti–PD-L1 treatment can remodel the macrophage compartment to enhance T-cell responses.
LIMS1 Promotes Pancreatic Cancer Cell Survival under Oxygen–Glucose Deprivation Conditions by Enhancing HIF1A Protein Translation
Authors: Huang Chongbiao , Li Yang , Li Zengxun , Xu Yang , Li Na , Ge Yi , Dong Jie , Chang Antao , Zhao Tiansuo , Wang Xiuchao , Wang Hongwei , Yang Shengyu , Xie Keping , Hao Jihui , Ren He 
Published: January 2019

Abstract

Purpose:
Oxygen and glucose deprivation is a common feature of the solid tumor. Regulatory network underlying the adaptation of cancer cells to the harsh microenvironment remains unclear. We determined the mechanistic role of LIM and senescent cell antigen-like–containing domain protein 1 (LIMS1) in cancer cell survival under oxygen–glucose deprivation conditions.

Experimental Design:
The expression level of LIMS1 was determined by IHC staining and analyzing the mRNA expression profiles from The Cancer Genome Atlas of three human solid tumors. Roles of LIMS1 in cancer cell metabolism and growth were determined by molecular and cell biology methods. A jetPEI nanocarrier was used as the vehicle for anti-LIMS1 siRNAs in mouse models of cancer therapeutics.

Results:
LIMS1 expression was drastically elevated in pancreatic ductal adenocarcinoma (PDAC). High LIMS1 level was associated with advanced TNM stage and poor prognosis of patients with tumor. Increased LIMS1 expression was pivotal for tumor cells to survive in the oxygen–glucose deprivation conditions. Mechanistically, LIMS1 enhanced GLUT1 expression and membrane translocation, which facilitated tumor cell adaptation to the glucose deprivation stress. Furthermore, LIMS1 promoted HIF1A protein translation by activating AKT/mTOR signaling, while hypoxia-inducible factor 1 (HIF1) transactivated LIMS1 transcription, thus forming a positive feedback loop in PDAC cell adaptation to oxygen deprivation stress. Inhibition of LIMS1 with jetPEI nanocarrier–delivered anti-LIMS1 siRNAs significantly increased cell death and suppressed tumor growth.

Conclusions:
LIMS1 promotes pancreatic cancer cell survival under oxygen–glucose deprivation conditions by activating AKT/mTOR signaling and enhancing HIF1A protein translation. LIMS1 is crucial for tumor adaptation to oxygen–glucose deprivation conditions and is a promising therapeutic target for cancer treatment.
EFFECT OF SALINITY ON THE SURVIVAL, GROWTH AND IMMUNITY RATE OF JUVENILE SEA CUCUMBERS (Holothuria scabra)
Authors: Sembiring Sari Budi Moria , Wibawa Gigih Setia , Hutapea Jhon Harianto , Giri I Nyoman Adiasmara 
Published: January 2019

Sea cucumber (Holothuria scabra) is important species as food and medicine especially for Chinese market. It Â is a stenohaline and osmoconforming organism with a low level of tolerance to salinity change. Salinity of the medium is one of the environmental factors that affect the physiology and survival of juvenile sea cucumbers. This study was aimed at assessing the effect of different salinities on the growth, survival and immunity rate of juvenile sea cucumbers (Holothuria scabra) and also determining the suitable salinity level for the optimal growth, survival and immunity rate of the sea cucumbers. ThisÂ experiment Â used a completely randomized design with 5 salinity treatments: 24, 29, 34, 39, and 44 ppt with 3 replications using fifteen30 L-fiber glass tanks. The juvenile sea cucumbers measured 4.4 Â± 0.2 cm in total length and 5.6 Â± 0.3 g in body weight. The juveniles were raised at 15 individuals/tank, fed with cultured fresh benthos once a day in the afternoon. Coelomate was taken from the sea cucumber juveniles from each tank Â and used to determinie the immunity rate and also for the osmolality. The data were analyzed using ANOVA followed by Tukeyâ€™s test at 5% level of significance. The salinity of the medium significantly affected (P < 0.05) the growth, survival rate and immunity rate of sea cucumber juveniles. The 24 - 34 ppt salinity can support survival rate up to 100%, high growth (6.47 â€“ 7.10 g) and immunity rate (27 â€“ 76Â x 104phagocytic cell/mL), while the 44 ppt salinity has resulted in not only a low survival rate (55.6%), but also had a bad effect on osmolality (303 Â± 3.5 mOs-mol kg-1), growth (3.12 Â± 0.34 g), and immunity (209 x 104 phagocytic cell/mL).
A survey of clinical laboratory instrument verification in the UK and New Zealand
Authors: Hand Matthew , Crampton Andrea , Thomas Annette , Kilpatrick Eric S 
Published: January 2019

Background Clinical laboratory instrument verification testing is often an accreditation requirement. However, it is not known what verification procedures are in routine use or how often the process identifies problems which need addressing prior to testing clinical samples. Objective To investigate which standards are currently being used for laboratory verification in UK and New Zealand (NZ) clinical laboratories and to help establish if the activity justifies the effort required. Methods A survey of verification of clinical laboratory instrumentation was distributed to members of the Association for Clinical Biochemistry and Laboratory Medicine and New Zealand Institute of Medical Laboratory Scientists. The survey consisted of questions on the verification elements used and whether acceptance criteria were met. Results Nineteen of 72 (26%) of responders only used organization-developed protocols for verification, 20/72 (28%) solely used national/international guidelines, while 16/72 (22%) used a combination. Manufacturers’ claims were partly or entirely used as acceptance criteria for imprecision (89%), accuracy (64%) and analytical measuring range (94%), with these being met on 61%, 67% and 93% of occasions, respectively. For patient comparison and linearity, acceptance criteria were met by 71% and 91%. Only 27–36% undertook any troubleshooting before accepting a failed component of verification. Conclusions Laboratories in the UK and NZ are currently using a variety of verification standards and acceptance criteria for instrument verification. It is common for instruments to fail, especially following the assessment of imprecision and accuracy. While this suggests the process is warranted, only a minority address failed elements before accepting verification.
Time Limitations on Applications for Judicial Review
Authors: Campbell Enid , Groves Matthew 
Published: January 2019

In most Australian jurisdictions there are limitations on the time within which proceedings for judicial review of governmental acts (or for particular remedies) are to be commenced. Some such limitations are imposed by statute, some by rules of court. In some cases the relevant court of supervisory jurisdiction is authorised to extend the time. But in other cases there is no discretion to extend the time.
This article examines the justifications which have been offered for imposition by legislation of limits on the time within which applications for judicial review are to be made. It surveys Australian legislation on the subject and examines the legal effects of such legislation. The article also considers reasons why it may be thought desirable to give courts authority to extend prescribed time limits and the associated question of whether exercise of that authority should be regulated by legislative standards. The article also examines the factors which the Federal Court has considered relevant in the exercise of its discretionary power to extend time under s 11 of the Administrative Decisions (Judicial Review) Act 1977 (Cth) (‘ADJR Act‘), a section which is substantially reproduced in s 10 of the Administrative Decisions (Judicial Review) Act 1989 (ACT), s 26 of the Judicial Review Act 1991 (Qld) and s 23 of the Judicial Review Act 2000 (Tas).
The Ritual and Aesthetic in Electoral Law
Authors: Orr Graeme 
Published: January 2019

Elections are commonplace events. Both in terms of their frequency, but also in the way they are taken for granted. Especially in Australia, but more generally in western democracies, their ebb and flow forms a seasonal rhythm, against which the more substantive work of government and public affairs – policy debates, law-making, administration and the (re)construction of community and national identities – is played out, through debate in the media, opinion polling and parliamentary and executive activity.The magic long ago seeped out of our understanding and, for many, the experience of elections. They are major logistical events, certainly. But on the surface they appear to be relatively routine affairs, professionally run by full-time, non-partisan electoral commissions, who are bureaucratically guided, indeed bound, by enormously detailed legislative codes. The Commonwealth Electoral Act 1918 (Cth) (CEA), for example, currently extends to more than 350 pages. By contrast, the inaugural Commonwealth Electoral Act 1902 (Cth) and accompanying Commonwealth Franchise Act 1902 (Cth) together spanned fewer than 45 pages.
Constitutional Law and the Limits of Discretion in Family Property Law
Authors: Parkinson Patrick 
Published: January 2019

AbstractThe argument of this Article is that the width of discretion that trial judges have to alter property rights under the Family Law Act 1975 (Cth) (‘the Act’) has been overstated. The property aspects of the Act can only be valid to the extent to which the law is an appropriate application of the marriage and divorce powers in the Constitution or is within the boundaries of the States’ reference of powers about de facto relationships. These constitutional provisions place significant constraints upon judicial discretion. In relation to marriages, the need to adjust property rights must result from the circumstances of the marital relationship or be justified as a consequence of the financial impact upon a party of its breakdown. The authority of Parliament to make laws concerning the alteration of the property rights of de facto partners is limited to cases of relationship breakdown.Furthermore, the Family Court of Australia and the Federal Circuit Court of Australia are both Chapter III courts. That has implications for the kind of discretion that Parliament can lawfully confer upon the trial judge, and the limits of that discretion. Some recent dicta and decisions of the Full Court of the Court suggest a view of judicial discretion which, it is argued, is inconsistent with the nature of judicial power in a Chapter III court. The discretion of trial judges is fettered by three duties: The duty to follow the interpretation of the Act as established authoritatively by appellate decisions, taking account of guidelines in appellate judgments; the duty to give reasons that explain the outcome of the case, and in particular, to justify the alteration of legal and equitable interests in specific items of property; and the duty to avoid arbitrary and capricious decision-making.The current jurisprudence on family property law is not necessarily consistent with these constitutional limitations.
Barking and Biting: The Equal Opportunity Commission as an Enforcement Agency
Authors: Allen Dominique 
Published: January 2019

AbstractFederal anti-discrimination law centres upon the individual who has experienced unlawful discrimination. To address this discrimination, the individual is required to lodge a complaint at the Australian Human Rights Commission (‘AHRC’), which will attempt to resolve the complaint using Alternative Dispute Resolution (‘ADR’). While institutions in other areas, like competition law and occupational health and safety, have a broad range of powers to enforce compliance, successive governments have chosen not to invest the AHRC with equivalent powers. Quite a different model has operated in Britain for four decades. This article analyses the role of the AHRC by comparing it to its British equivalents and examining these institutions according to the ‘enforcement pyramid’ for regulating equal opportunity, which British academics Bob Hepple, Mary Coussey and Tufyal Choudhury have developed. According to these regulatory theorists, to tackle discrimination effectively, equality commissions need to be able to follow up their loud ‘bark’ with a punitive ‘bite’ if necessary. The article concludes by identifying what the experience in both countries reveals about the enforcement of antidiscrimination laws by statutory institutions.
History and Memory in Constitutional Adjudication
Authors: Barak-Erez Daphne 
Published: January 2019

AbstractThis article considers the different ways in which judicial decisions use and narrate history. It distinguishes between several forms of judicial recourse to history, including the difference between decisions which refer to general history and decisions that refer to the history of legal documents; and the difference between decisions on factual controversies that have historical significance and decisions that take judicial notice of history. At the same time, this article recognises that the division between these categories is not clear-cut. An analysis of constitutional case law sheds light on the ways in which courts harness historical events in order to justify their normative choices. More specifically, while some judicial decisions cite history in order to justify continuity with the past, others regard history as a cautionary tale that calls for a change of direction. In between, some decisions opt for a middle route, supporting continuity with historical decisions but offering new interpretations of their lessons. This article concludes by examining decisions that try to ‘learn’ from history, illuminating the enduring challenge in drawing different and even conflicting lessons from the very same historical event.
Statutory Misinterpretation: Rash Holding in Brash Holdings
Authors: Fishman Paulina 
Published: January 2019

AbstractThe modern approach to statutory construction guides the judiciary, the legal profession, litigants, and academics in interpreting the myriad legislative provisions in Australian law. Yet what if critical sections have been construed in ways that are irreconcilable with the basic rules of modern statutory interpretation? One of the most important commercial statutes in the country is the Corporations Act 2001 (Cth). This article exposes one instance of misinterpretation in respect of that statute, contained in a decision of a unanimous Full Court of the Supreme Court of Victoria, and makes proposals for resolving such quandaries.
Accountability in Regulatory Reform: Australia's Superannuation Industry Paradox
Authors: Taylor Sue , Asher Anthony , Tarr Julie Anne 
Published: January 2019

AbstractThe Australian Superannuation Industry is generally seen as very strong and successful by global standards. However, three decades of legislative reform in the Australian superannuation industry have created a paradox: ongoing reforms but continuing dissatisfaction with areas of governance and outcomes. These include high levels of administrative and investment fees, and systematic problems around a culture of conflicted investment advice.In seeking to further elaborate and then resolve this paradox, this article draws upon an extensive research project conducted by the authors within the Australian superannuation industry, including three voluntary and anonymous surveys of superannuation trustees/licensees. This research has revealed that fund members are vulnerable to significant and expanding private-interest rents generated by the financial services sector. This may be explained by regulatory capture mechanisms variously described as statutory, agency, corrosive and intellectual capture.The article examines ways to better achieve public interest outcomes, and at a time when public sector integrity remains an area of particular attention, how more can be done to blunt the force of private interest rent seeking.
The Perils and Pitfalls of Formal Equality in Australian Family Law Reform
Authors: Fehlberg Belinda , Sarmas Lisa , Morgan Jenny 
Published: January 2019

AbstractIn this paper, we identify the influence of formal equality—and more specifically, formal gender equality (that is, treating men and women the same)—in central areas of major Australian family law reform over the past 20 years. Given the influence of formal equality and our concerns regarding this trend, we consider whether equality-based arguments should be abandoned entirely, at least in the family law context, and explore alternative approaches that could reframe the debate.
The ‘Modern Approach’ to Statutory Interpretation and the Principle of Legality: An Issue of Coherence?
Authors: Meagher Dan 
Published: January 2019

AbstractThe ‘modern approach’ to interpretation requires that effect be given to the ordinary meaning of a statutory text in its wider context and with reference to its purpose. This article interrogates the interpretive approach used by the High Court in fundamental rights cases to assess its compatibility with this ‘modern approach’. It asks, specifically, whether the strict text-based approach used in these cases (which involves the application of the principle of legality) is sufficient to determine the meaning of ambiguous statutory texts. The argument made is that the coherent application of the ‘modern approach’ requires that this interpretive approach be justified.
Some Problems of Commonwealth Immunity and Exclusive Legislative Powers
Authors: Howard Colin 
Published: January 2019

There is a well-known obscurity in the law relating to Commonwealth immunity from State legislative power. It is that whereas the High Court has laid down in clear terms that the Commonwealth is not subject to State legislative power at all, it has at the same time said that the Commonwealth may be “affected by” State laws but has not explained what is meant by “affected by”.There is also a less well-known obscurity in the law relating to State taxation of Common\vealth public servants. The High Court has held that general State tax laws apply to Commonwealth public servants in the same way as to other subjects of the State, unless the Commonwealth enacts legislation exempting the payments which it makes to its public servants by way of salary or pension from State taxation. The obscurity is that a Commonwealth law prescribing salaries for its own public servants appears to be an exercise of exclusive legislative power; that a law exempting such salaries from State taxation appears to be incidental to that power, and therefore itself an exercise of exclusive power; but that if this is so, the State tax law also, in its application to Commonwealth salaries, appears to be an exercise of Commonwealth exclusive power and therefore invalid in the first place.
The Gove Land Rights Case: A Judicial Dispensation for the Taking of Aboriginal Lands in Australia?
Authors: Hookey John 
Published: January 2019

In 1971, Blackburn J., delivering a swingeing judgment in the Supreme Court of the Northern Territory, held that the members of three Arnhem I and clans had no legally recognizable rights in their ancestral lands, and thereby decided what was thought to be the first case brought by Australian aboriginals seeking legal recognition of their customary land rights.British settlements were first established in Australia in 1788. Ever since it is a notorious fact that aboriginals have been consistently deprived of their land by settlers, miners and governments. The surprising thing therefore, to lawyers unfamiliar with the history of Australian race relations, is that aboriginal rights in land do not appear to have been asserted in the courts by aboriginal plaintiffs before the present proceedings began. The absence of reported cases dealing specifically with the rights of Australian aboriginals in their lands is all the more exceptional in the light of the wide range of decisions on indigenous claims in other murisdictions.
The Structure and Nature of Australian Environmental Law
Authors: Whalan Douglas J. 
Published: January 2019

Constitutional authority over resources and environmental questions resides principally in the States in Australia, but there are many powers which the Federal Government can develop, either alone or together with the States, in working out national policies in these areas. Although the constitutional issues are important, resolution of land use problems is fundamentally a matter of competition for power and authority within the political system.Professor Whalan suggests four stages in the development of environmental law: (a) totally resource-oriented statutes with possible overlaps and clashes between separate uses; (b) integration of the separate codes and the introduction into those codes of environmentally oriented protective provisions; (c) the enactment of statutes directed towards a specific environmental problem or problems; and (d) the development of overview bodies with advisory and supervisory functions to which the protective responsibilities may be added.In these statutory moves towards macro-environmental law in Australia, the Courts have been given a very limited role and it is argued that much of the law is not normative in character but procedural, administrative and discretionary. In very many cases, ultimate control is political rather than judicial.