bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acupuncture induces adenosine in fibroblasts through energy metabolism and promotes proliferation by activating MAPK signaling pathway via adenosine3 receptor
Authors: Qu Fei , Cui Yanru , Zeng Jie , Zhang Mingyue , Qiu Shaying , Huang Xiaoting , Chen Aishe 
Published: September 2019

AbstractAcupuncture has many advantages in the treatment of certain diseases as opposed to drug therapy. Besides, adenosine has been revealed to affect cellular progression including proliferation. Therefore, this study aimed at exploring the mechanism involving acupuncture stress and adenosine in fibroblast proliferation. The fibroblasts from fascia tissues of the acupoint area (Zusanli) were stimulated by different levels of stress, different concentrations of adenosine, and agonist or antagonist of A3 receptor (A3R) to investigate the effect of stress stimulation, adenosine, and adenosine‐A3R inhibition on fibroblasts. Then, the fibroblasts were treated with stress stimulation of 200 kPa or/and mitogen‐activated protein kinase (MAPK) blocker. We revealed that stress stimulation and the binding of adenosine and A3R promoted fibroblast proliferation in the fascial tissue, increased the expression of immune‐related factors, adenosine and A3R, and activated the MAPK signaling pathway. MAPK signaling pathway also directly affected the expression of adenosine, A3R, and immune‐related factors. Stress stimulation and adenosine treatment upregulated A3R expression, and then activated the MAPK signaling pathway, which could in turn upregulate expression of adenosine, A3R and immune‐related factors, and promote cell proliferation. Adenosine is shown to form a positive feedback loop with the MAPK signaling pathway. Collectively, stress stimulation in vitro induces the increase of adenosine in fibroblasts through the energy metabolism and activation of the MAPK signaling pathway through A3R, ultimately promoting fibroblast proliferation.
Human leukocytes differentially express endocannabinoid-glycerol lipases and hydrolyze 2-arachidonoyl-glycerol and its metabolites from the 15-lipoxygenase and cyclooxygenase pathways
Authors: Turcotte Caroline , Dumais Élizabeth , Archambault Anne-Sophie , Martin Cyril , Blanchet Marie-Renée , Bissonnette Élyse , Boulet Louis-Philippe , Laviolette Michel , Di Marzo Vincenzo , Flamand Nicolas 
Published: September 2019

Abstract
2-Arachidonoyl-glycerol (2-AG) is an endocannabinoid with anti-inflammatory properties. Blocking 2-AG hydrolysis to enhance CB2 signaling has proven effective in mouse models of inflammation. However, the expression of 2-AG lipases has never been thoroughly investigated in human leukocytes. Herein, we investigated the expression of seven 2-AG hydrolases by human blood leukocytes and alveolar macrophages (AMs) and found the following protein expression pattern: monoacylglycerol (MAG lipase; eosinophils, AMs, monocytes), carboxylesterase (CES1; monocytes, AMs), palmitoyl-protein thioesterase (PPT1; AMs), α/β-hydrolase domain (ABHD6; mainly AMs), ABHD12 (all), ABHD16A (all), and LYPLA2 (lysophospholipase 2; monocytes, lymphocytes, AMs). We next found that all leukocytes could hydrolyze 2-AG and its metabolites derived from cyclooxygenase-2 (prostaglandin E2-glycerol [PGE2-G]) and the 15-lipoxygenase (15-hydroxy-eicosatetraenoyl-glycerol [15-HETE-G]). Neutrophils and eosinophils were consistently better at hydrolyzing 2-AG and its metabolites than monocytes and lymphocytes. Moreover, the efficacy of leukocytes to hydrolyze 2-AG and its metabolites was 2-AG ≥ 15-HETE-G &gt;&gt; PGE2-G for each leukocyte. Using the inhibitors methylarachidonoyl-fluorophosphonate (MAFP), 4-nitrophenyl-4-(dibenzo[d][1,3]dioxol-5-yl(hydroxy)methyl)piperidine-1-carboxylate (JZL184), Palmostatin B, 4′-carbamoylbiphenyl-4-yl methyl(3-(pyridin-4-yl)benzyl)carbamate, N-methyl-N-[[3-(4-pyridinyl)phenyl]methyl]-4′-(aminocarbonyl)[1,1′-biphenyl]-4-yl ester carbamic acid (WWL70), 4′-[[[methyl[[3-(4-pyridinyl)phenyl]methyl]amino]carbonyl]oxy]-[1,1′-biphenyl]-4-carboxylic acid, ethyl ester (WWL113), tetrahydrolipstatin, and ML349, we could not pinpoint a specific hydrolase responsible for the hydrolysis of 2-AG, PGE2-G, and 15-HETE-G by these leukocytes. Furthermore, JZL184, a selective MAG lipase inhibitor, blocked the hydrolysis of 2-AG, PGE2-G, and 15-HETE-G by neutrophils and the hydrolysis of PGE2-G and 15-HETE-G by lymphocytes, two cell types with limited/no MAG lipase. Using an activity-based protein profiling (ABPP) probe to label hydrolases in leukocytes, we found that they express many MAFP-sensitive hydrolases and an unknown JZL184-sensitive hydrolase of ∼52 kDa. Altogether, our results indicate that human leukocytes are experts at hydrolyzing 2-AG and its metabolites via multiple lipases and probably via a yet-to-be characterized 52 kDa hydrolase. Blocking 2-AG hydrolysis in humans will likely abrogate the ability of human leukocytes to degrade 2-AG and its metabolites and increase their anti-inflammatory effects in vivo.
Does Low Body Mass Index Predict Mortality in Asian Hospitalized Patients?
Authors: Xue Zhigang , Yu Jianchun , Higashikuchi Takashi , Compher Charlene 
Published: September 2019

AbstractBackgroundStudies on malnutrition suggest low body mass index (BMI) may be associated with poor clinical outcomes. We investigated published data to identify whether low BMI could predict short‐term and long‐term clinical outcomes in Asian hospitalized patients.MethodsA search was conducted in the PubMed database including terms “body mass index” or “BMI” or “malnutrition” or “undernutrition” AND “Asia” or “Asian” AND “outcome” or “complication” or “mortality” or “survival” or “ICU” for publications in English in the past 10 years. Papers with data and clinical outcomes from Asian hospitalized patients with BMI <18.5 kg/m2 and sample size >50 were included. Forest plots were constructed to determine the odds of hospital complications, hospital mortality, intensive care unit (ICU) mortality, and 5‐year mortality in patients with BMI <18.5 kg/m2 vs BMI ≥18.5 kg/m2.ResultsTwenty‐three studies were included, 16 of which were analyzed for the association between low BMI and clinical outcomes. The prevalence of BMI <18.5 kg/m2 varied from 3% to 48% in different disease settings and Asian areas. Patients with BMI <18.5 kg/m2 had significantly higher ICU mortality (odds ratio [OR], 1.92; 95% CI, 1.84–2.01), higher hospital mortality (OR, 2.48; 95% CI, 2.42–2.55), and higher 5‐year mortality (OR, 2.74; 95% CI, 2.08–3.63). Complications during hospital stay were significantly higher in patients with BMI <18.5 kg/m2 (OR, 1.99; 95% CI, 1.65–2.40).ConclusionsLow BMI defined as <18.5 kg/m2 may predict worse short‐term and longer‐term outcomes in Asian hospitalized patients.
CD147 self‐regulates matrix metalloproteinase‐2 release in gingival fibroblasts after coculturing with U937 monocytic cells
Authors: Lai Tat‐Ming , Kuo Po‐Jan , Lin Chi‐Yu , Chin Yu‐Tang , Lin Hsiao‐Lun , Chiu Hsien‐Chung , Fu Martin M.J. , Fu Earl 
Published: September 2019

AbstractBackgroundCluster of differentiation 147 (CD147) is a multifunctional glycoprotein that functions as an inducer of matrix metalloproteinase (MMP) expression in fibroblasts. Synergistically enhanced MMP‐2 expression was recently observed in the coculture of human gingival fibroblasts (HGFs) and U937 human monocytic cells; however, the responsible mechanisms have not yet been fully established. The aim of this study was to evaluate the release of soluble CD147 in HGFs after coculturing with U937 cells and its functional effect on the enhancement of MMP‐2 expression in HGFs.MethodsEnzyme‐linked immunosorbent assay was used to determine the amount of CD147 protein in media, whereas real‐time polymerase chain reaction was performed to evaluate the mRNA levels of CD147 and MMP‐2 in HGFs and U937 cells. The enzyme activities of MMP‐2 released from cells were examined by zymography. Transwell coculturing and conditioned media treatments were selected to rule out the effect of direct contact of HGFs and U937 cells.ResultsThe protein and mRNA expression of CD147 in HGFs were enhanced after transwell coculturing with U937 cells and exposure to U937‐conditioned medium. MMP‐2 enzyme activities in HGFs were also significantly increased by the coculturing methods. Administration of exogenous CD147 enhanced MMP‐2 expression in HGFs, whereas treatment with cyclosporine‐A, which inhibited CD147 expression, reduced U937‐enhanced MMP‐2 expression in HGFs.ConclusionsCD147 can interact with fibroblasts to stimulate the expression of MMPs associated with periodontal extracellular matrix degradation. This study has demonstrated that CD147 released from fibroblasts might play a role in monocyte‐enhanced MMP‐2 expression in HGFs.
Carbonized silk fibers for in‐tube solid‐phase microextraction to detect polycyclic aromatic hydrocarbons in water samples
Authors: Ji Xiangping , Sun Min , Li Chunying , Han Sen , Guo Wenjuan , Feng Juanjuan 
Published: September 2019

AbstractSilk fibers were carbonized to develop a biomass carbon material as an adsorbent for solid‐phase microextraction. The surface structure of the carbonized silk fibers was characterized by scanning electron microscopy, and the graphitization degree was determined by Raman spectrometry. After carbonization under high temperature, the orderliness and structural regularity of carbon atoms on silk fibers were promoted. Extraction tube packed with carbonized silk fibers was prepared for in‐tube solid‐phase microextraction. Coupled with high performance liquid chromatography, it exhibited good extraction performance for hydrophobic polycyclic aromatic hydrocarbons. Main parameters including sampling volume, sampling rate, methanol content in sample, and desorption time were systematically investigated. Under the optimum conditions, the analysis method was established and it exhibited wide linear range (0.016–20 μg/L) with good linearity (correlation coefficient ≥ 0.9947), low limits of detection (0.005–0.050 μg/L), and high enrichment factors (1189–2775). Relative standard deviations (n = 3) for intraday (≤3.3%) and interday (≤9.6%) tests indicated that the extraction material had satisfactory repeatability. Finally, the analytical method was successfully applied to detect trace polycyclic aromatic hydrocarbons in real water samples, demonstrating its satisfactory practicability.
Core‐shell structured magnetic mesoporous carbon nanospheres derived from metal‐polyphenol coordination polymer‐coated Fe3O4 and its application in the enrichment of phthalates from water samples
Authors: Xue Shan , Wang Yiyu , Wu Dan , Shen Jiwei , Wei Yinmao , Wang Chaozhan 
Published: September 2019

AbstractIn this work, core‐shell structured magnetic mesoporous carbon nanospheres were fabricated from the carbonization of metal‐polyphenol coordination polymer‐coated Fe3O4 nanoparticles. The preparation method is simple, fast, versatile, and easy to scale up. Magnetic mesoporous carbon nanospheres exhibit a high specific surface area, high superparamagnetism, and high adsorption efficiencies for phthalates. Four phthalates were extracted from aqueous solutions by using magnetic mesoporous carbon nanospheres via magnetic solid phase extraction. Subsequent analysis was performed by using high‐performance liquid chromatography with ultraviolet detection. The analytical method has good linearity in the concentration range of 1–200 ng/mL for diethyl phthalate, diisobutyl phthalate, and dicyclohexyl phthalate, and 3–200 ng/mL for dipropyl phthalate. The limits of detection were in the range of 0.10–0.62 ng/mL. Compared with previous methods, this method has a lower detection limit, wider linearity range, and faster adsorption and desorption rates. The results indicate that magnetic mesoporous carbon nanospheres are suitable for the enrichment of hydrophobic substances from aqueous solutions.
A collaborative approach to bridging the gap between wildlife managers and researchers
Authors: Merkle Jerod A. , Anderson Neil J. , Baxley Danna L. , Chopp Matthew , Gigliotti Laura C. , Gude Justin A. , Harms Tyler M. , Johnson Heather E. , Merrill Evelyn H. , Mitchell Michael S. , Mong Tony W. , Nelson Jerry , Norton Andrew S. , Sheriff Michael J. , Tomasik Eric , VanBeek Kelly R. 
Published: September 2019

ABSTRACTAlthough most wildlife professionals agree that science should inform wildlife management decisions, disconnect still exists between researchers and managers. If researchers are not striving to incorporate their findings into management decisions, support for research programs by managers can wane. If managers are not using research findings to inform management decisions, those decisions may be less effective or more vulnerable to legal challenges. Both of these situations can have negative consequences for wildlife conservation. We outline a collaborative research‐management approach to bridging the gap between wildlife managers and researchers. We describe differences in perspectives, perceptions, and priorities between managers and researchers; outline how and why the divide between researchers and managers has likely occurred and continues to grow; and present specific strategies and recommendations to foster stronger collaborations between managers and researchers. We advocate increased synergy between managers and researchers based on a shared vision of conservation and a collaborative structure that rewards researchers and managers. Most importantly, we suggest that relationships and communication between managers and researchers must be established early in research development and decision‐making processes, fostering the trust needed for collaboration. Institutions and agencies can facilitate these relationships by creating opportunities and incentives for integrating collaborative research into management decisions. We suggest this approach will strengthen ties between researchers and managers, increase relevance of research to management decisions, promote effectiveness of management decisions, reduce legal challenges, and ultimately produce positive, tangible, and lasting effects on wildlife conservation. © 2019 The Wildlife Society.
Recognizing consumerism as an “illness of an empty soul”: A catholic morality perspective
Authors: Azevedo António 
Published: September 2019

AbstractCatholics personalist authors have been always concerned about consumerism, which is considered a radical dissatisfaction that compulsively tries to fill the emptiness of the inner self. It is a form of idolatry where malls are modern cathedrals. What are the antecedents and consequences of this moral approach, inducing consumers’ engagement in anti‐consumption behaviors? This conceptual paper updates the literature review about this research topic, acknowledging the thoughts written in encyclical letters of three Popes of Catholic Church: John Paul II, Benedict XVI, and Francis. This paper also proposes a new model of ethical decision‐making model that aims to describe the implications of recognizing consumerism as a moral/spiritual issue according to the Catholic Church teaching. This paper provides useful insights into the influence of Catholic teaching on the adoption of anti‐consumption behaviors.
Anti‐TNF‐α therapy alters the gut microbiota in proteoglycan‐induced ankylosing spondylitis in mice
Authors: Liu Bin , Yang Lianjun , Cui Zhifei , Zheng Junchi , Huang Jincheng , Zhao Qinghao , Su Zhihai , Wang Min , Zhang Weicong , Liu Jinshi , Wang Tingxuan , Li Qingchu , Lu Hai 
Published: September 2019

AbstractAnkylosing spondylitis is a chronic, progressive disease, and its treatment is relevant to the gut microbiota. Anti‐tumor necrosis factor‐alpha (anti‐TNF‐α) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease. However, little is known about the effect of TNF‐α blocker treatment on the gut microbiota in ankylosing spondylitis. Herein, the effect of a TNF‐α blocker on the gut microbiota in proteoglycan‐induced arthritis was investigated. Proteoglycan‐induced mice were treated with an rhTNFR:Fc solution of etanercept (5 µg/g) for 4 weeks. rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan‐induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction. Moreover, high gut permeability occurred, and zonula occludens‐1 and occludin protein levels were reduced in proteoglycan‐induced mice. These levels were significantly restored by the administration of rhTNFR:Fc. The serum TNF‐α and IL‐17 levels were also decreased. In addition, flora analysis via 16S rDNA high‐throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice. In conclusion, anti‐TNF‐α therapy attenuated proteoglycan‐induced arthritis progression and modulated the gut microbiota and intestinal barrier function. These results provide new insights for anti‐TNF‐α therapy strategies via regulating the gut microbiota in ankylosing spondylitis.
Composite MRA: statistical approach to generate an MR angiogram from multiple contrasts
Authors: Kim Dahan , Edjlali Myriam , Turski Patrick , Johnson Kevin M. 
Published: September 2019

PurposeTo develop a method to use information from multiple MRI contrasts to produce a composite angiogram with reduced sequence‐specific artifacts and improved vessel depiction.MethodsBayesian posterior vessel probability was determined as a function of black blood (BB), contrast enhanced angiography (CE‐MRA), and phase‐contrast MRA (PC‐MRA) intensities from training subjects (N = 4). To generate composite angiogram in evaluation subjects (N = 12), the voxel‐wise vessel probabilities were weighted with a confidence measure and combined as a weighted product to yield angiogram intensity. For 23 internal carotid artery (ICA) segments (N = 23) from evaluation subjects, segmentation accuracy of composite MRA was evaluated and compared against CE‐MRA using dice similarity coefficient (DSC).ResultsThe composite MRA suppressed venous contaminations in CE‐MRA, reduced flow artifacts, and velocity aliasing seen in PC‐MRA and removed signal ambiguities in BB images. For ICA segmentations, the composite MRA improved segmentation over CE‐MRA per DSC (0.908 ± 0.037 vs. 0.765 ± 0.079). Compared with CE‐MRA, the composite MRA showed conservative changes in vessel appearance to small threshold changes. However, small vessels that are sensitive to registration errors or visible only weakly in CE‐MRA were susceptible to poor depiction in composite MRA.ConclusionBy dynamically weighting vessel information from multiple contrasts and extracting their complementary information, the composite MRA produces reduced sequence‐specific artifacts and improved vessel contrast. It is a promising technique for semi‐automatic segmentation of vessels that are hard to segment because of artifacts.
Bariatric Surgery Leads to Short‐Term Effects on Sweet Taste Sensitivity and Hedonic Evaluation of Fatty Food Stimuli
Authors: Nielsen Mette S. , Andersen Ida Ninett S.K. , Lange Belinda , Ritz Christian , le Roux Carel W. , Schmidt Julie B. , Sjödin Anders , Bredie Wender L.P. 
Published: September 2019

ObjectiveThe aim of this study was to investigate the effect of bariatric surgery on sweet taste sensitivity and the hedonic evaluation of sweet, savory, and fatty food stimuli as well as associations with weight loss and food preferences assessed at a buffet meal test.MethodsThe detection and recognition threshold for sweet taste and the hedonic rating of sweet, savory, and fatty food stimuli were assessed before and after a preoperative diet‐induced weight loss and 6 weeks, 6 months, and 18 months after Roux‐en‐Y gastric bypass (RYGB) and sleeve gastrectomy (SG) (n = 29).ResultsThe detection threshold for sweet taste decreased after the diet‐induced weight loss and 6 weeks after RYGB (both P ≤ 0.03). The hedonic rating of high‐fat food stimuli decreased 6 weeks after RYGB and SG (all P ≤ 0.02). Changes in the hedonic rating of high‐fat food stimuli were associated with increased preferences for high‐fat foods at the buffet meal (P = 0.03) and tended to be associated with weight loss (P = 0.05). No changes were detected for sweet and savory food stimuli.ConclusionsRYGB increased sweet taste sensitivity; however, this effect was already seen after the diet‐induced weight‐loss. RYGB and SG decreased the hedonic evaluation of high‐fat food stimuli, but this effect did not translate into decreased preferences for high‐fat food.
Disordered hemostasis associated with severely depressed fibrinolysis demonstrated using a simultaneous thrombin and plasmin generation assay during L‐asparaginase induction therapy in pediatric acute lymphoblastic leukemia
Authors: Ishihara Takashi , Nogami Keiji , Ochi Satoshi , Ishida Toshiaki , Kosaka Yoshiyuki , Sawada Akihisa , Inoue Masami , Osone Shinya , Imamura Toshihiko , Hosoi Hajime , Shima Midori 
Published: September 2019

AbstractBackgroundL‐asparaginase (L‐Asp)‐associated thromboembolisms are serious complications in pediatrics patients with acute lymphoblastic leukemia (ALL), especially at ≥10.0 years old, but the pathogenesis remains to be clarified.ProcedureWe conducted a multicenter, prospective study of 72 patients with ALL aged 1.0 to 15.2 years treated with either a Berlin‐Frankfurt‐Münster (BFM) 95‐ALL oriented regimen or Japan Association of Childhood Leukemia Study ALL‐02 protocol. We divided patients into each treatment protocol and investigated the dynamic changes in coagulation and fibrinolysis using simultaneous thrombin and plasmin generation assay. Patients’ plasma samples were collected at the prephase (T0), intermittent phase (T1), and postphase of L‐Asp therapy (T2), and postinduction phase (T3). Measurements of endogenous thrombin potential (T‐EP) and plasmin peak height (P‐Peak) were compared to normal plasma.ResultsNone of the cases developed thromboembolisms. Median ratios of T‐EP and P‐Peak for the controls in the JACLS group were 1.06 and 0.87 (T0), 1.04 and 0.71 (T1), 1.02 and 0.69 (T2), and 1.20 and 0.92 (T3), respectively, while those in the BFM group were 1.06 and 1.00 (T0), 1.04 and 0.64 (T1), 1.16 and 0.58 (T2), and 1.16 and 0.85 (T3), respectively. In particular, P‐Peak ratios were depressed at T1 and T2 compared to T0 in the BFM group (P < .01). Moreover, P‐Peak ratios in patients ≥10.0 years old were lower at T1 in the BFM group (P = .02).ConclusionsThe results demonstrated that hemostatic dynamics appeared to shift to a hypercoagulable state with marked hypofibrinolysis associated with L‐Asp therapy, especially in patients ≥10.0 years old following the BFM regimen.