bluuebunny/crossref_metadata_2025_split huggingface dataset:
S1PR1 suppresses lung adenocarcinoma progression through p-STAT1/miR-30c-5 p/FOXA1 pathway
Authors: Chai Yanfei , Xiang Hong , Ma Yuchao , Feng Wei , Jiang Zhibin , Zhu Qianjun , Chen Yingji , Liu Quanjun , Zhang Jing , Ouyang Jie , Gao Peng , Zhang Xiao , Chen Shuhua , Jin Longyu , Lu Hongwei 
Published: November 2024

Abstract
Background
Sphingosine-1-phosphate receptor 1 (S1PR1) is considered to be closely related to a variety of malignant tumors, but the role and mechanism of S1PR1 in lung adenocarcinoma are not fully understood. In this study, we aim to explore the role and downstream signaling pathways of S1PR1 in the malignant biological functions of lung adenocarcinoma (LUAD).

Methods
Bioinformatics analysis, RT-qPCR, western blot and immunohistochemistry (IHC) were was used to investigate the expression of S1PR1 in LUAD. The prognosis of S1PR1 was also analyzed. CCK-8 assay, colony formation assay, scratch assay, transwell migration and invasion assay, cell adhesion assay were performed to examine the effect of S1PR1 on LUAD. RNA sequencing was employed to analyze the DEGs in LUAD cells overexpressing S1PR1. Enrichment pathway analysis using KEGG, GO, and GSEA was conducted to predict potential signaling pathways and downstream targets. chromatin immunoprecipitation (ChIP) and dual luciferase reporter assay were performed to verify the direct regulation between FOXA1 and the target genes. Then FOXA1 overexpression were performed to functional rescue experiments. miRNA-30c-5p was identified as a microRNA regulating FOXA1 by dual luciferase reporter assay. The downstream signaling pathways of S1PR1 was detected to clarify the specific pathways to regulates miR-30c-5p.

Results
S1PR1 is significantly decreased in LUAD and is positively correlated with the prognosis. Overexpression of S1PR1 inhibits the proliferation, migration, invasion and adhesion function of LUAD cells by suppressing the expression of COL5A1, MMP1, and SERPINE1. FOXA1 is a key transcription factor regulating the expression of MMP1, COL5A1 and SERPINE1. S1PR1 inhibits the expression of FOXA1 through p-STAT1/miR-30c-5p, thereby suppressing the malignant function of LUAD cells.

Conclusions
The expression of S1PR1 is downregulated in LUAD, which is positively correlated with prognosis. S1PR1 regulates the malignant function of LUAD cells by inhibiting the expression of COL5A1, MMP1 and SERPINE1 through the p-STAT1/miR-30c-5p/FOXA1 signaling pathway.
Therapeutic effects of extracellular vesicles derived from mesenchymal stem cells primed with disease-conditioned-immune cells in systemic lupus erythematosus
Authors: Choi Eun Wha , Shin Il Seob , Lim I-Rang , Lee Jihye , Choi Bongkum , Kim Sungjoo 
Published: November 2024

Abstract
Background
Systemic lupus erythematosus (SLE) is an incurable chronic autoimmune disease of unknown etiology. Therefore, the development of new treatments is urgently needed. This study aimed to investigate the therapeutic effects of extracellular vesicles (EV) derived from immortalized mesenchymal stem cells (iMSCs) primed with conditioned media obtained from disease-conditioned immune cells (CM-EV) and iMSC-derived EV (ASC-EV) in a murine model of SLE.

Methods
Female NZB/W F1 mice were divided into the control (C, n = 15), ASC-EV (E, n = 15), and CM-EV (CM, n = 15) groups. Mice in the C, E, and CM groups were intravenously administered saline, ASC-EV, and CM-EV, respectively, once weekly from 6 to 42 weeks of age.

Results
Compared to the ASC-EV, the CM-EV showed a significant increase in TGF-β1 production and miR-155-5p and miR-142-3p expression. CM-EV treatment increased survival, decreased anti-dsDNA antibody levels, and ameliorated renal histopathology. Although ASC-EV treatment significantly reduced the incidence of severe proteinuria and improved renal histopathology, it did not significantly improve survival rate.
ASC-EV or CM-EV treatment significantly decreased the proportion of pro-inflammatory macrophages (CD11c + CD206-; M1) and M1:M2 ratio. Additionally, CM-EV treatment significantly increased the expression of anti-inflammatory macrophages (CD11c-CD206 + ; M2). Moreover, CM-EV treatment significantly decreased the expression of lupus-specific miRNAs (miR-182-5p and miR-183-5p) in the spleen.

Conclusions
EV derived from iMSCs primed with conditioned media obtained from disease-conditioned immune cells exert immunomodulatory effects and ameliorate SLE in a murine model.
Does targeted information impact consumers’ preferences for value-based health insurance? Evidence from a survey experiment
Authors: Bardy Tess L. C. , Boes Stefan 
Published: November 2024

Abstract
Objectives
Value-based insurance design (VBID) aims to direct consumers’ preferences by incentivizing the use of high-value care and discouraging the use of low-value care. However, consumers often have limited knowledge of health insurance and the health insurance system, possibly distorting their preferences. In this study, we aim to investigate the impact of specific information treatments on consumers’ preferences for VBID.

Methods
We implemented an information experiment as part of a representative survey on health insurance literacy and preferences for VBID within Switzerland’s choice-based health insurance system. Preferences for VBID were measured through a discrete choice experiment. Cross-sectional data on 6,033 respondents aged 26–75 were analyzed using descriptive statistics and mixed logit regressions.

Results
Respondents showed strong preferences for their current health insurance instead of VBID alternatives. A general description of current regulations on cost-sharing, drug disbursement, and monthly premiums significantly increased preferences for VBID (p < 0.01). Pointing respondents specifically to VBID further reduced the opposition against VBID plans. At the same time, there is evidence for anchoring effects in copayments after receiving the information treatments, irrespective of the value of the care.

Conclusions
The results of this study highlight that individuals are susceptible to provided information about health insurance when building their preferences for VBID. One potential explanation is limited health insurance literacy, implying that tailored communication strategies may be needed to improve insurance decision-making.

JEL Classification
I11, I13.
Experimental and numerical study on fire development process and fire risk assessment of historic timber lounge bridges
Authors: Cui Zhekui , Chun Qing 
Published: November 2024

Abstract
Historic timber lounge bridges are precious architectural heritages. But they are sensitive to fire. In order to obtain an adequate understanding of the fire vulnerability of historical timber lounge bridges for their better conservation, this paper proposes a framework based on experiment and computational fluid dynamics (CFD) analysis. Dengyun Bridge, a typical timber lounge bridge with cantilever beams, was chosen to carry out study on the fire development process and fire risk assessment. Firstly, the structural form and fire loads of the bridge were obtained by conducting on-site survey, and then a complex pyrolysis model of ancient wood was established in the Fire Dynamic Simulator (FDS) according to the pyrolysis characteristic of the ancient wood replaced from the bridge during its renovation. Secondly, the fire development process in Dengyun Bridge was simulated and four crucial fire events were defined. Finally, the comprehensive fire risk index for the Dengyun Bridge under those fire events was calculated by applying the Analytic hierarchy process (AHP) and Entropy method, and some mitigation and prevention strategies were discussed accordingly. The obtained results indicated that the flashover point was a boundary of fire development. After the flashover, the comprehensive fire risk index increased and reached its peak value rapidly. In the 1084 s (peak heat release rate), the comprehensive fire risk index was about 1.7 times of that in 847 s (flashover point). Mitigation strategies, such as the flame-retardant treatment and water-mist extinguishing system, were proved to be effective to retard the developed fire. The results of this study can provide a scientific basis for fire risk assessment and conservation of inherited timber lounge bridges.
Seeking orderness out of the orderless movements: an up-to-date review of the biomechanics in clear aligners
Authors: Li Jiawei , Si Jiarui , Xue Chaoran , Xu Hui 
Published: November 2024

Abstract
Introduction
Although with increasing popularity due to aesthetic appeal and comfort, clear aligners (CAs) are facing challenges in efficacy and predictability. Advancement in the underlying biomechanical field is crucial to addressing these challenges. This paper endeavors to provide a comprehensive framework for understanding the biomechanics of CA and enlightening biomechanics-based improvements on treatment strategies.

Methods
A thorough review of the English-language literature accessible through PubMed and Google Scholar, without any publication year restrictions, was undertaken to unravel the biomechanical aspects of CA.

Results
This review presented an up-to-date understanding of aligner biomechanics arranged by the framework of the material-dependent mechanical characteristics of CA, the geometric characteristics-dependent force transmission of the CA system, methods for studying the biomechanics of CA, and the biomechanical analyses for different types of tooth movement.

Conclusions
Biomechanics should be the fundamental concern for concepts, methods and adjuncts attempting to enhance the accuracy and predictability of tooth movement induced by CA. Improvement on material properties and alteration of geometric design of CA are two main approaches to develop biomechanically optimized force system. Exploration of real-world force sensing and monitoring system would make substantial progresses in aligner biomechanics.
Circular RNAs: novel noncoding players in male infertility
Authors: Babakhanzadeh Emad , Hoseininasab Fakhr-Alsadat , Khodadadian Ali , Nazari Majid , Hajati Reza , Ghafouri-Fard Soudeh 
Published: November 2024

AbstractInfertility is a global problem being associated with emotional and financial burden. Recent studies have shown contribution of a group of non-coding RNAs, namely circular RNAs (circRNAs) to the etiology of some infertility conditions. CircRNA are transcribed from exons and form a circular RNA molecule, being abundant in eukaryotes. Traditionally classified as non-coding RNA, these transcripts are endogenously produced through either non-canonical back-splicing or linear splicing, typically produced from precursor messenger ribonucleic acid (pre-mRNA). While during the canonical splicing process the 3’ end of the exon is joined to the 5’ end of the succeeding exon to form linear mRNA, during backsplicing, the 3’ end to the 5’ end of the same exon is joined to make a circular molecule. circRNAs are involved in the regulation of several aspects of spermatogenesis. They appear to influence how stem germ cells grow and divide during the sperm production process. Malfunctions in circRNA activity could contribute to male infertility issues stemming from abnormalities in spermatogenesis. In the current review, we highlight the exciting potential of circRNAs as key players in the male fertility.
Assessment of podocyte detachment as a pivotal step in the development of focal segmental glomerulosclerosis
Authors: Elkholy Ikbal Ahmed Abdo , Elkashef Wagdi , Mostafa Fatma El-Husseini , Hassan Amany 
Published: November 2024

Abstract
Background
Podocytopenia refers to a decrease in the number of podocytes. When podocytes are injured, they may detach leading to podocytopenia, which represents a critical step in the development of podocytopathy and subsequently deterioration of renal functions. Pathological assessment of podocytopenia plays a crucial role in diagnosing underlying kidney diseases.

Aim
To assess detached podocytes and evaluate their diagnostic role in the development of focal segmental glomerulosclerosis.

Materials and methods
This is a retrospective study, conducted on 67 archival renal biopsies with the clinical diagnosis of steroid-resistant or steroid-dependent nephrotic syndrome (SRNS) and diagnosed as focal segmental glomerulosclerosis (FSGS) and podocytopathy with detached podocytes by electron microscopy (EM). Colloidal iron stain and Desmin immunohistochemical stain were performed. Assessment of the mean percent of stained pixels in relation to the surface tuft area of the glomerulus, i.e., mean percent of stained area (PSA) was done using image analysis system (ImageJ 1.52a) software.

Results
Podocytopathy with detached podocytes was diagnosed in 35 (52.24%) cases, while FSGS was diagnosed in 32 (47.76%) cases. Regarding detached podocytes, 27 (49.3%) cases showed no detached podocytes by light microscopy (LM), while only 4 (6%) showed severe podocyte detachment. There was a statistically significant difference between control cases and both podocytopathy with detached podocytes and FSGS regarding mean PSA (p ≤ 0.001).

Conclusion
Standardized reporting of detached podocyte cells is becoming mandatory as they have a high positive predictive value for the expected EM picture.
Understanding neuropathic pain: the role of neurophysiological tests in unveiling underlying mechanisms
Authors: Leone Caterina Maria , Truini Andrea 
Published: November 2024

AbstractNeuropathic pain, arising from lesions of the somatosensory nervous system, presents with diverse symptoms including ongoing pain, paroxysmal pain, and provoked pain, usually accompanied by sensory deficits. Understanding the pathophysiological mechanisms behind these symptoms is crucial for targeted treatment strategies. Neurophysiological techniques such as nerve conduction studies, reflexes, and evoked potentials help elucidate these mechanisms by assessing large myelinated non-nociceptive fibres and small nociceptive fibres. This argumentative review highlights the importance of tailored neurophysiological assessments for improving our understanding of the pathophysiological mechanisms behind neuropathic pain symptoms.
Clinical Relevance of Intensive Laboratory Monitoring With Standard Venetoclax Ramp-Up for Chronic Lymphocytic Leukemia: A Real-World Experience
Authors: Huang Ivan J. , Baek Grace T. , Cohen Jonathan , Khajaviyan Sirin , Louie Stephanie , Samples Laura , Smith Stephen D. , Till Brian G. , Warren Edus H. , Gopal Ajay K. , Poh Christina , Lynch Ryan C. , Ujjani Chaitra S. , Shadman Mazyar 
Published: November 2024

PURPOSE
Venetoclax is the standard of care for chronic lymphocytic leukemia (CLL)/small lymphocytic lymphoma (SLL) but requires intensive monitoring for optimal safety. Clinical relevance of intensive monitoring in practice is unknown, especially for patients with low or intermediate risk for tumor lysis syndrome (TLS).

PATIENTS AND METHODS
A retrospective review was conducted to determine clinical significance of monitoring for TLS during standard ramp-up for patients with CLL/SLL. Patients receiving abbreviated ramp-up, clinical trials, or concurrent Bruton tyrosine kinase inhibitors were excluded. The primary end point was TLS incidence, with secondary end points describing associated clinical interventions.

RESULTS
Fifty-five patients met study criteria. The majority of patients received venetoclax as first-line therapy (58%), with anti-CD20 antibody therapy (82%), and were at low risk of TLS (75%). No clinical TLS events occurred, whereas laboratory TLS occurred in only 1.8% of patients. No patients required antihyperuricemic therapy, and few interventions for hyperphosphatemia or hypocalcemia (3.6% of patients) were required. Additional intravenous fluids were uncommonly required (1.8% of patients), and no unplanned hospitalizations were required.

CONCLUSION
These findings support efforts to reduce intensive monitoring requirements during venetoclax ramp-up for patients with CLL, potentially increasing accessibility of venetoclax.
Sensitivity of two-mode SRF cavity to generic electromagnetism of sub-μeV dark matter
Authors: Dai Chang-Jie , Li Tong , Zhang Rui-Jia 
Published: November 2024

Abstract
The low-mass dark matter (DM) such as axion or wave-like scalar is a plausible DM candidate. Recently, the possible non-standard couplings of low-mass DM has drawn much attention. In this work we investigate the detection of electromagnetic couplings in a few benchmark models of low-mass DM. For illustration, we consider the generic axion electrodynamics including CP-violating coupling as well as the newly proposed axion electromagnetodynamics. The superconducting radio frequency (SRF) cavity with two modes has more advantages than the traditional cavity approach with static background field. We utilize the two-mode SRF cavity to probe the generic couplings of low-mass DM with frequency lower than GHz. We show the sensitivity of the SRF cavity to the axion couplings in the above frameworks.
Using Artificial Intelligence to Identify Three Presenting Phenotypes of Chiari Type-1 Malformation and Syringomyelia
Authors: Gupta Vivek Prakash , Xu Ziqi , Greenberg Jacob K. , Strahle Jennifer Mae , Haller Gabriel , Meehan Thanda , Roberts Ashley , Limbrick David D. , Lu Chenyang 
Published: November 2024

BACKGROUND AND OBJECTIVES:
Chiari type-1 malformation (CM1) and syringomyelia (SM) are common related pediatric neurosurgical conditions with heterogeneous clinical and radiological presentations that offer challenges related to diagnosis and management. Artificial intelligence (AI) techniques have been used in other fields of medicine to identify different phenotypic clusters that guide clinical care. In this study, we use a novel, combined data-driven and clinician input feature selection process and AI clustering to differentiate presenting phenotypes of CM1 + SM.

METHODS:
A total of 1340 patients with CM1 + SM in the Park Reeves Syringomyelia Research Consortium registry were split a priori into internal and external cohorts by site of enrollment. The internal cohort was used for feature selection and clustering. Features with high Laplacian scores were identified from preselected groups of clinically relevant variables. An expert clinician survey further identified features for inclusion that were not selected by the data-driven process.

RESULTS:
The feature selection process identified 33 features (28 from the data-driven process and 5 from the clinician survey) from an initial pool of 582 variables that were incorporated into the final model. A K-modes clustering algorithm was used to identify an optimum of 3 clusters in the internal cohort. An identical process was performed independently in the external cohort with similar results. Cluster 1 was defined by older CM1 diagnosis age, small syringes, lower tonsil position, more headaches, and fewer other comorbidities. Cluster 2 was defined by younger CM1 diagnosis age, more bulbar symptoms and hydrocephalus, small syringes, more congenital medical issues, and more previous neurosurgical procedures. Cluster 3 was defined by largest syringes, highest prevalence of spine deformity, fewer headaches, less tonsillar ectopia, and more motor deficits.

CONCLUSION:
This is the first study that uses an AI clustering algorithm combining a data-driven feature selection process with clinical expertise to identify different presenting phenotypes of CM1 + SM.
T-Bar Angular Offset: A Novel Angle for Predicting Postoperative Coronal Balance Using Intraoperative Stitched Radiographs
Authors: Zhou James J. , Farber S. Harrison , Gooldy Timothy C. , Furey Charuta G. , Cho Steve S. , Ehresman Jeff , O’Neill Luke K. , Uribe Juan S. , Kakarla U. Kumar , Turner Jay D. 
Published: November 2024

BACKGROUND AND OBJECTIVES:
Accurate intraoperative assessment of coronal alignment is critical to achieving favorable clinical outcomes in adult spinal deformity surgery. However, surgical positioning creates challenges in predicting standing coronal alignment. Gravity-based plumblines require an upright posture and are not possible intraoperatively. Intraoperative stitched radiography (ISR) is increasingly being used to evaluate coronal balance as an alternative to 36-inch films, which can be cumbersome. However, ISR does not allow for the measurement of absolute distances, which limits the intraoperative assessment of the coronal plane. We propose a novel angle to assess coronal balance on ISR: the T-bar angular offset (TAO). We evaluate TAO as a measure of coronal balance and assess the degree to which TAO measured on ISR can predict postoperative coronal balance.

METHODS:
Patients undergoing spinal fusion surgery with ISR were identified retrospectively. A virtual T-bar was created by drawing a line bisecting the S1 endplate perpendicular to a line connecting the acetabula. A second line was drawn from the origin of the first to the midpoint of the superior endplate of the upper instrumented vertebra (UIV) or C7. The angles subtended by these lines were defined as the UIV TAO and C7 TAO, respectively.

RESULTS:
Sixty-eight patients were identified, with a median (range) of 8 (3-21) levels fused. Intraoperative UIV TAO correlated with both postoperative UIV TAO (R = 0.695, P < .001) and postoperative UIV central sacral vertical line (R = 0.435, P = .002). Intraoperative C7 TAO did not correlate with either postoperative C7 TAO (R = 0.213, P = .22) or C7 central sacral vertical line (R = 0.063, P = .72).

CONCLUSION:
TAO is a useful angular measure that can be used on noncalibrated ISR to predict the positioning of the UIV relative to the sacrum during spinal fusion surgery. However, it does not appear to be predictive of the coronal alignment of C7.
Loneliness or Solitude: Which Will We Experience?
Authors: Rokach Ami , Boulazreg Samir 
Published: November 2024

Loneliness, social isolation, aloneness, and solitude are frequently used interchangeably but are actually different. Loneliness is particularly salient now, due to the international restrictions on social activities imposed as a result of COVID-19, which brought loneliness into open discussion worldwide. The article highlights loneliness as a multidimensional construct and reviews its impact on cognitive, behavioral and affective functioning. In doing so, particular attention is given to loneliness as it manifests through the various life stages, as well as how personal predisposition and contextual factors may exacerbate it. in this article we also review solitude, and a clear distinction between loneliness and solitude is established. Finally, we conclude by addressing the global claims of loneliness during the pandemic and its implications. We offer a point of view which may assist in coping with it.
The Importance of Unemployment Risk for Individual Savings
Authors: Juelsrud Ragnar E. , Wold Ella Getz 
Published: November 2024

In this paper, we use a novel natural experiment and Norwegian tax data to quantify the causal impact of unemployment risk on individual savings. By comparing individuals who live in the same area but face different increases in risk, we show that a one-percentage-point increase in unemployment rates increases safe assets by 1.3%. Reassuringly, this effect is driven by low-tenured workers, who face the highest increase in risk. Savings in risky financial assets remain unaffected, implying a decrease in the overall risky share of individual portfolios. We use two independent approaches, relying either on cross-sectional variation or time series variation, to quantify the importance of job loss risk in accounting for higher savings during recessions. Our results suggest that unemployment risk can explain 60%–80% of the recession-induced increase in safe assets. This paper was accepted by Camelia Kuhnen, finance. Supplemental Material: The data files are available at https://doi.org/10.1287/mnsc.2023.00987 .