bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intermittent screening and treatment with dihydroartemisinin-piperaquine and intermittent preventive therapy with sulfadoxine-pyrimethamine have similar effects on malaria antibody in pregnant Malawian women
Authors: Teo Andrew , Randall Louise M. , Madanitsa Mwayiwawo , Mwapasa Victor , Phiri Linda Kalilani , Khairallah Carole , Buffet Christelle , Karahalios Amalia , Narum David L. , Kuile Feiko O. Ter , Rogerson Stephen J. 
Published: May 2019

AbstractIn a randomised trial comparing intermittent screening and treatment (IST) with dihydroartemisinin-piperaquine (DP) and intermittent preventive therapy against malaria in pregnancy (IPT) with sulfadoxine-pyrimethamine (SP) in Malawi, the impacts of IST-DP and IPT-SP on the development and maintenance of malaria antibody immunity were compared. Pregnant Malawian women were randomised to receive IST-DP or IPT-SP. In a nested study, paired enrolment and delivery plasma samples from 681 women were assayed for antibodies against recombinant antigens and for IgG and opsonising antibodies to antigens found on infected erythrocytes (IEs). At delivery, antibody responses did not differ between study arms. Between enrolment and delivery, antibodies to recombinant antigens decreased, whereas antibodies to IEs including opsonising antibodies remained stable. Overall, changes in antibody responses over pregnancy did not differ by treatment arm. Stratifying by gravidity, antibody to schizont extract decreased more in multigravidae receiving IST-DP than IPT-SP. There was minimal impact of treatment arm on the development and maintenance of malaria immunity. While antibodies to recombinant antigens declined between enrolment and delivery, antibodies directed against IEs tended to be more stable, suggesting longer-lasting protection.Clinical trial registration: Pa n African Clinical Trials Registry (PACTR201103000280319) 14/03/2011. URL: http://www.isrctn.com/ISRCTN69800930.
Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum
Authors: Anani Hussein , Abou Abdallah Rita , Chelkha Nisrine , Fontanini Anthony , Ricaboni Davide , Mailhe Morgane , Raoult Didier , Fournier Pierre-Edouard 
Published: May 2019

AbstractWe used phenotypic, genomic and phylogenetic information following the taxono-genomics approach to demonstrate that strain Marseille–P3254, isolated from an ileal sample of a 76-year old woman who underwent upper and lower digestive tract endoscopy for esophagitis and colonic polyp, is representative of a novel bacterial genus within the family Erysipelotrichaceae in the phylum Firmicutes. It is an anaerobic Gram-negative bacterium without catalase and oxidase activities. The genome of strain Marseille–P3254 is 2,468,496-bp long with a 40.1% G + C content. This new bacterium is most closely related to Eubacterium dolichum, with which it shares 90.7% 16S rRNA sequence similarity. In addition, genomic comparison using the digital DNA–DNA hybridization and OrthoANI analyses between the novel organism and the E. dolichum type strain revealed identities of 25.2 and 68.91%, respectively. The major fatty acids were C16: 0, C18: 1n9 and C18: 0. Based on these data, we propose the creation of the new genus Merdibacter gen. nov., with strain Marseille-P3254T (=CSUR P3254 = DSM 103534) being the type strain of the new species Merdibacter massiliensis gen. nov., sp. nov.
Gastric bypass surgery in lean adolescent mice prevents diet-induced obesity later in life
Authors: Mumphrey Michael B. , Hao Zheng , Leigh Townsend R. , Qualls-Creekmore Emily , Yu Sangho , Lutz Thomas A. , Münzberg Heike , Morrison Christopher D. , Berthoud Hans-Rudolf 
Published: May 2019

AbstractGastric bypass surgery is the most effective treatment and is often the only option for subjects with severe obesity. However, investigation of critical molecular mechanisms involved has been hindered by confounding of specific effects of surgery and side effects associated with acute surgical trauma. Here, we dissociate the two components by carrying out surgery in the lean state and testing its effectiveness to prevent diet-induced obesity later in life. Body weight and composition of female mice with RYGB performed at 6 weeks of age were not significantly different from sham-operated and age-matched non-surgical mice at the time of high-fat diet exposure 12 weeks after surgery. These female mice were completely protected from high-fat diet-induced obesity and accompanying metabolic impairments for up to 50 weeks. Similar effects were seen in male mice subjected to RYGB at 5–6 weeks, although growth was slightly inhibited and protection from diet-induced obesity was less complete. The findings confirm that RYGB does not indiscriminately lower body weight but specifically prevents excessive diet-induced obesity and ensuing metabolic impairments. This prevention of obesity model should be crucial for identifying the molecular mechanisms underlying gastric bypass surgery.
Activation of the unfolded protein response in high glucose treated endothelial cells is mediated by methylglyoxal
Authors: Irshad Zehra , Xue Mingzhan , Ashour Amal , Larkin James R. , Thornalley Paul J. , Rabbani Naila 
Published: May 2019

AbstractMetabolic dysfunction of endothelial cells in hyperglycemia contributes to the development of vascular complications of diabetes where increased reactive glycating agent, methylglyoxal (MG), is involved. We assessed if increased MG glycation induced proteotoxic stress, identifying related metabolic drivers and protein targets. Human aortal endothelial cells (HAECs) were incubated in high glucose concentration (20 mM versus 5 mM control) in vitro for 3–6 days. Flux of glucose metabolism, MG formation and glycation and changes in cytosolic protein abundances, MG modification and proteotoxic responses were assessed. Similar studies were performed with human microvascular endothelial HMEC-1 cells where similar outcomes were observed. HAECs exposed to high glucose concentration showed increased cellular concentration of MG (2.27 ± 0.21 versus 1.28 ± 0.03 pmol/106 cells, P < 0.01) and formation of MG-modified proteins (24.0 ± 3.7 versus 14.1 ± 3.2 pmol/106 cells/day; P < 0.001). In proteomics analysis, high glucose concentration increased proteins of the heat shock response – indicating activation of the unfolded protein response (UPR) with downstream inflammatory and pro-thrombotic responses. Proteins susceptible to MG modification were enriched in protein folding, protein synthesis, serine/threonine kinase signalling, glycolysis and gluconeogenesis. MG was increased in high glucose by increased flux of MG formation linked to increased glucose metabolism mediated by proteolytic stabilisation and increase of hexokinase-2 (HK-2); later potentiated by proteolytic down regulation of glyoxalase 1 (Glo1) - the major enzyme of MG metabolism. Silencing of Glo1, selectively increasing MG, activated the UPR similarly. Silencing of HK-2 prevented increased glucose metabolism and MG formation. trans-Resveratrol and hesperetin combination (tRES-HESP) corrected increased MG and glucose metabolism by increasing expression of Glo1 and decreasing expression of HK-2. Increased MG glycation activates the UPR in endothelial cells and thereby may contribute to endothelial cell dysfunction in diabetic vascular disease where tRES-HESP may provide effective therapy.
A repetitive acidic region contributes to the extremely rapid degradation of the cell-context essential protein TRIM52
Authors: Hacker Kathrin , Benke Stefan , Agerer Benedikt , Scinicariello Sara , Budroni Valentina , Versteeg Gijs A. 
Published: May 2019

AbstractTripartite motif protein 52 (TRIM52) is a non-canonical TRIM family member harbouring the largest RING domain encoded in the human genome. In humans TRIM52 is conserved and has been under positive selection pressure, yet it has been lost in many non-primates. Competitive cell fitness assays demonstrated that TRIM52 ablation reduces cellular fitness in multiple different cell types. To better understand how this cell-essential factor is controlled, we investigated how expression of this non-canonical protein is regulated. Here, we show that TRIM52 mRNA is constitutively expressed from an intergenic region preceding the TRIM52 gene. Yet, TRIM52 protein is rapidly turned-over by the proteasome with a 3.5-minute half-life, one of the shortest in the human proteome. Consistent with this extremely rapid degradation rate, all three TRIM52 domains were identified to contribute to its instability. Intriguingly, a repetitive acidic loop in the RING domain was identified as one of the main destabilizing regions, which was unexpected given the prevailing notion that these sequences are poor proteasome substrates. This work indicates that the effect of such repetitive acidic regions on proteasomal degradation depends on the protein context, and it identifies TRIM52 as an attractive model protein to study what these contextual properties are.
Estimating global ocean heat content from tidal magnetic satellite observations
Authors: Irrgang Christopher , Saynisch Jan , Thomas Maik 
Published: May 2019

AbstractOcean tides generate electromagnetic (EM) signals that are emitted into space and can be recorded with low-Earth-orbiting satellites. Observations of oceanic EM signals contain aggregated information about global transports of water, heat, and salinity. We utilize an artificial neural network (ANN) as a non-linear inversion scheme and demonstrate how to infer ocean heat content (OHC) estimates from magnetic signals of the lunar semi-diurnal (M2) tide. The ANN is trained using monthly OHC estimates based on oceanographic in-situ data from 1990–2015 and the corresponding computed tidal magnetic fields at satellite altitude. We show that the ANN can closely recover inter-annual and decadal OHC variations from simulated tidal magnetic signals. Using the trained ANN, we present the first OHC estimates from recently extracted tidal magnetic satellite observations. Such space-borne OHC estimates can complement the already existing in-situ measurements of upper ocean temperature and can also allow insights into abyssal OHC, where in-situ data are still very scarce.
Wound fluids collected postoperatively from patients with breast cancer induce epithelial to mesenchymal transition but intraoperative radiotherapy impairs this effect by activating the radiation-induced bystander effect
Authors: Kulcenty Katarzyna , Piotrowski Igor , Zaleska Karolina , Wichtowski Mateusz , Wróblewska Joanna , Murawa Dawid , Suchorska Wiktoria Maria 
Published: May 2019

AbstractWound fluids (WF) are believed to play a role in the local recurrences by inducing an inflammatory process in scar tissue area. Given that most local relapse in primary breast cancer patients occur within the scar tissue area, researchers have investigated whether localized radiotherapy, such as intraoperative radiotherapy (IORT), could be more effective than postoperative RT in inhibiting local tumor recurrence. The epithelial-mesenchymal transition (EMT) program plays a critical role in promoting metastasis in epithelium-derived carcinoma. Given this background the main aim of the present study was to determine the mechanisms by which IORT decreases the tumorigenic potential of WF. We assumed that postoperative fluids from patients would activate the radiation-induced bystander effect (RIBE) in treated cells, thus altering the tumor microenvironment. To confirm this hypothesis, WF collected from patients after breast conserving surgery (BCS) alone, after BCS followed by IORT treatment or WF from BCS patients together with RIBE medium were incubated with MCF7 and MDA-MB-468 cells. Changes in the CSC phenotype, in EMT program and potential to migrate were performed to determine the possible role of WF on the migration of breast cancer cells. Our findings show that wound fluids stimulate the CSC phenotype and EMT program in breast cancer cell lines. This effect was partially abrogated when the cells were incubated in wound fluids collected from patients after breast-conserving surgery followed by IORT. Additionally, we confirmed the role of radiation-induced bystander effect in altering the properties of the WF to induce the CSC phenotype and EMT program.
Deep Learning Convolutional Neural Networks for the Automatic Quantification of Muscle Fat Infiltration Following Whiplash Injury
Authors: Weber Kenneth A. , Smith Andrew C. , Wasielewski Marie , Eghtesad Kamran , Upadhyayula Pranav A. , Wintermark Max , Hastie Trevor J. , Parrish Todd B. , Mackey Sean , Elliott James M. 
Published: May 2019

AbstractMuscle fat infiltration (MFI) of the deep cervical spine extensors has been observed in cervical spine conditions using time-consuming and rater-dependent manual techniques. Deep learning convolutional neural network (CNN) models have demonstrated state-of-the-art performance in segmentation tasks. Here, we train and test a CNN for muscle segmentation and automatic MFI calculation using high-resolution fat-water images from 39 participants (26 female, average = 31.7 ± 9.3 years) 3 months post whiplash injury. First, we demonstrate high test reliability and accuracy of the CNN compared to manual segmentation. Then we explore the relationships between CNN muscle volume, CNN MFI, and clinical measures of pain and neck-related disability. Across all participants, we demonstrate that CNN muscle volume was negatively correlated to pain (R = −0.415, p = 0.006) and disability (R = −0.286, p = 0.045), while CNN MFI tended to be positively correlated to disability (R = 0.214, p = 0.105). Additionally, CNN MFI was higher in participants with persisting pain and disability (p = 0.049). Overall, CNN’s may improve the efficiency and objectivity of muscle measures allowing for the quantitative monitoring of muscle properties in disorders of and beyond the cervical spine.
Reduced Plasma Orexin-A Concentrations are Associated with Cognitive Deficits in Anorexia Nervosa
Authors: Steward Trevor , Mestre-Bach Gemma , Granero Roser , Sánchez Isabel , Riesco Nadine , Vintró-Alcaraz Cristina , Sauchelli Sarah , Jiménez-Murcia Susana , Agüera Zaida , Fernández-García Jose C. , Garrido-Sánchez Lourdes , Tinahones Francisco J. , Casanueva Felipe F. , Baños Rosa M. , Botella Cristina , Crujeiras Ana B. , Torre Rafael de la , Fernández-Real Jose M. , Frühbeck Gema , Ortega Francisco J. , Rodríguez Amaia , Menchón José M. , Fernández-Aranda Fernando 
Published: May 2019

AbstractOrexins/hypocretins are neuropeptides implicated in numerous processes, including food intake and cognition. The role of these peptides in the psychopathology of anorexia nervosa (AN) remains poorly understood. The aim of the current study was to evaluate the associations between plasma orexin-A (OXA) concentrations and neuropsychological functioning in adult women with AN, and a matched control group. Fasting plasma OXA concentrations were taken in 51 females with AN and in 51 matched healthy controls. Set-shifting was assessed using the Wisconsin Card Sorting Test (WCST), whereas decision making was measured using the Iowa Gambling Task (IGT). The AN group exhibited lower plasma OXA levels than the HC group. Lower mean scores were obtained on the IGT in AN patients. WCST perseverative errors were significantly higher in the AN group compared to HC. In both the AN and HC group, OXA levels were negatively correlated with WCST non-perseverative errors. Reduced plasma OXA concentrations were found to be associated with set-shifting impairments in AN. Taking into consideration the function of orexins in promoting arousal and cognitive flexibility, future studies should explore whether orexin partly underpins the cognitive impairments found in AN.
Fabrication of laser printed microfluidic paper-based analytical devices (LP-µPADs) for point-of-care applications
Authors: Ghosh Rajesh , Gopalakrishnan Saranya , Savitha Rangasamy , Renganathan Thiruvengadam , Pushpavanam Subramanium 
Published: May 2019

AbstractMicrofluidic paper-based analytical devices (µPADs) have provided a breakthrough in portable and low-cost point-of-care diagnostics. Despite their significant scope, the complexity of fabrication and reliance on expensive and sophisticated tools, have limited their outreach and possibility of commercialization. Herein, we report for the first time, a facile method to fabricate µPADs using a commonly available laser printer which drastically reduces the cost and complexity of fabrication. Toner ink is used to pattern the µPADs by printing, without modifying any factory configuration of the laser printer. Hydrophobic barriers are created by heating the patterned paper which melts the toner ink, facilitating its wicking into the cross-section of the substrate. Further, we demonstrate the utilization of the fabricated device by performing two assays. The proposed technique provides a versatile platform for rapid prototyping of µPADs with significant prospect in both developed and resource constrained region.
Prevalence of metabolic syndrome and its determinants among Iranian adults: evidence of IraPEN survey on a bi-ethnic population
Authors: Jahangiry Leila , Khosravi-far Leila , Sarbakhsh Parvin , Kousha Ahmad , EntezarMahdi Rasool , Ponnet Koen 
Published: May 2019

AbstractMetabolic syndrome (MetS) is a growing public health concern worldwide. It has been demonstrated that individuals with MetS are at an increased risk of cardiovascular events and diabetes. We aimed to investigate the prevalence of MetS and its components among Turkic and Kurds ethnic groups in a bi-ethnic (Turk and Kurd) population. This cross-sectional study is part of the national health transformation plan created in response to the emerging epidemic of non-communicable diseases (Iran’s Package of Essential Non-communicable Disease study), launched in 2014 in Naqadeh, Iran. In total, 3506 participants aged 30–70 years were randomly included in the study from urban and rural regions. Cardio-metabolic risk factors related to MetS diagnosis and other related sociodemographic factors were assessed for men and women in both the Turk and the Kurd population. Multivariate logistic regressions were applied to identify MetS -associated factors among both the Turk and the Kurd population. The mean (SD) age of the participants was 49.6 (12.3) years. Of the participants, 56.2% (n = 1969) were women, and 43.8% (n = 1537) were men. Three-fifths of the participants were Turk (60.3%, n = 1751). The overall prevalence of MetS was 37.05%, with a higher prevalence in women (49.8% versus 24.3% in men). The prevalence of MetS and its components among Turk people (41.6%) were significantly higher than that among Kurd people (33.9%) (p < 0.0001). In addition, the prevalence of MetS was higher among women, urban, and older people for both ethnicities. Strong associations were found between MetS prevalence and being older, being female, being overweight, being obese, having a higher waist-to-hip ratio, and having a history of diabetes and cardiovascular disease (CVD) in the family for both Turks and Kurds. The raised waist circumference (WC) is the most prevalent MetS component for Turk men and women. Meanwhile, the most prevalent MetS component for Kurd participants is low high-density lipoprotein for women and a raised WC for men. Significant differences were found between Kurdish men and women for all components, except for a raised WC and a raised fasting blood glucose (p < 0.05). Because the Iranian population features multiple ethnicities, the recognition of the prevalence of MetS components is a major step in establishing intervention strategies for modifying cardio-metabolic risk factors based on the population ethnicities and their socio-demographic, cultural, and lifestyle factors. We recommend future studies for planning an efficient and sustainable health education and promotion program to halt MetS prevalence.
Synthesis of corannulene-based nanographenes
Authors: Muzammil Ezzah M. , Halilovic Dzeneta , Stuparu Mihaiela C. 
Published: May 2019

AbstractCorannulene (C20H10) is a polycyclic hydrocarbon in which five six-membered rings surround a central five-membered ring to construct a bowl-like aromatic structure. Here we examine the development of synthetic strategies that allow for the growth of the peripheral aromatic rings as a means to extend the aromatic area of the central corannulene nucleus and provide access to unique nanocarbon molecules.
ExPRES: an Exoplanetary and Planetary Radio Emissions Simulator
Authors: Louis C. K. , Hess S. L. G. , Cecconi B. , Zarka P. , Lamy L. , Aicardi S. , Loh A. 
Published: May 2019

Context. Earth and outer planets are known to produce intense non-thermal radio emissions through a mechanism known as cyclotron maser instability (CMI), requiring the presence of accelerated electrons generally arising from magnetospheric current systems. In return, radio emissions are a good probe of these current systems and acceleration processes. The CMI generates highly anisotropic emissions and leads to important visibility effects, which have to be taken into account when interpreting the data. Several studies have shown that modelling the radio source anisotropic beaming pattern can reveal a wealth of physical information about the planetary or exoplanetary magnetospheres that produce these emissions.
Aims. We present a numerical tool, called ExPRES (Exoplanetary and Planetary Radio Emission Simulator), which is able to reproduce the occurrence in a time-frequency plane of R−X CMI-generated radio emissions from planetary magnetospheres, exoplanets, or star–planet interacting systems. Special attention is given to the computation of the radio emission beaming at and near its source.
Methods. We explain what physical information about the system can be drawn from such radio observations, and how it is obtained. This information may include the location and dynamics of the radio sources, the type of current system leading to electron acceleration and their energy, and, for exoplanetary systems, the orbital period of the emitting body and the strength, rotation period, tilt, and the offset of the planetary magnetic field. Most of these parameters can only be remotely measured via radio observations.
Results. The ExPRES code provides the proper framework of analysis and interpretation for past, current, and future observations of planetary radio emissions, as well as for future detection of radio emissions from exoplanetary systems (or magnetic, white dwarf–planet or white dwarf–brown dwarf systems). Our methodology can be easily adapted to simulate specific observations once effective detection is achieved.