bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ledipasvir‐Sofosbuvir for 12 Weeks in Children 3 to <6 Years Old With Chronic Hepatitis C
Authors: Schwarz Kathleen B. , Rosenthal Philip , Murray Karen F. , Honegger Jonathan R. , Hardikar Winita , Hague Rosie , Mittal Naveen , Massetto Benedetta , Brainard Diana M. , Hsueh Chia‐Hsiang , Shao Jiang , Parhy Bandita , Narkewicz Michael R. , Rao Girish S. , Whitworth Suzanne , Bansal Sanjay , Balistreri William F. 
Published: June 2019

For children under 12 years of age who have chronic hepatitis C virus (HCV) infection, there are currently no approved treatments with direct‐acting antiviral agents. We therefore evaluated the safety and efficacy of ledipasvir‐sofosbuvir in HCV‐infected children aged 3 to <6 years. In an open‐label study, patients 3 to <6 years old chronically infected with HCV genotype 1 (n = 33) or 4 (n = 1) received weight‐based doses of combined ledipasvir‐sofosbuvir as granules (33.75 mg/150 mg for weights <17 kg or 45 mg/200 mg for weights ≥17 kg) for 12 weeks. The primary endpoint was sustained virological response 12 weeks after treatment (SVR12). For the first 14 patients, intensive pharmacokinetic sampling was done on day 10 of treatment. All patients had been infected through perinatal transmission and were treatment naïve. No patients had known cirrhosis. Ten patients (29%) weighed <17 kg. SVR12 was achieved in 97% of patients (33 of 34); the patient who did not achieve SVR12 was 3 years old and discontinued treatment after 5 days because of an adverse event “abnormal drug taste.” The most common adverse events were vomiting (24% of patients), cough (21%), and pyrexia (21%). No patients experienced a serious adverse event. Intensive pharmacokinetic analysis of 13 patients for whom data were evaluable confirmed that the doses selected were appropriate. Conclusion: Ledipasvir‐sofosbuvir was well tolerated and highly effective in children 3 to <6 years old with chronic HCV infection.
Total Recoverable Aluminum: Not Totally Relevant for Water Quality Standards
Authors: Ryan Adam C , Santore Robert C , Tobiason Scott , WoldeGabriel Giday , Groffman Armand R 
Published: June 2019

Abstract
A large water quality data set, representing more than 100 surface-water locations sampled from 2007 to 2017 in the Los Alamos area of New Mexico, USA's Pajarito Plateau, was assembled to evaluate Al concentrations in unfiltered and filtered samples. Aluminum concentrations often exceeded United States Environmental Protection Agency (USEPA) and New Mexico ambient water quality criteria (AWQC), regardless of filter size and sample location. However, AWQC are based on laboratory toxicity studies using soluble Al salts and do not reflect natural conditions in Pajarito Plateau surface waters. The plateau is predominately covered by glassy and recrystallized volcanic ashes (e.g., Bandelier Tuff) containing colloidal to sand-sized aluminosilicates. Samples from natural background drainages and areas downstream of developed regions exhibited similar Al concentrations, suggesting that AWQC exceedances are caused by naturally elevated Al concentrations. Solubility calculations indicated that most samples were oversaturated with respect to amorphous Al(OH)3(s). Therefore, AWQC exceedances are likely artifacts of the “total recoverable” sample preparation, which includes acidification and partial digestion, thereby liberating nonbioavailable Al from aluminosilicates. Accordingly, Al concentrations were strongly associated with suspended sediment concentrations (SSCs), implying that aluminosilicates in suspended sediment contributed to AWQC exceedances and Al oversaturation. Solid-phase particle characterization, using X-ray diffraction (XRD) and scanning electron microscopy with electron dispersive spectroscopy (SEM/EDS) did not identify potentially bioavailable amorphous Al(OH)3(s) in any sample tested. Thus, current sample collection and analysis protocols should not be used to evaluate attainment of Al AWQC on the Pajarito Plateau or locations where aluminosilicates are substantial contributors to total recoverable Al. A sample preparation method (e.g., pH 4 extraction) capable of differentiating nonbioavailable and bioavailable forms of Al is recommended. Otherwise, current New Mexico and USEPA sample preparation approaches will continue to generate artifactual AWQC exceedances in surface waters that contain aluminosilicates. Integr Environ Assess Manag 2019;00:1–14. © 2019 SETAC
SIMULATION OF SOIL WATER CONTENT FOR IRRIGATION MANAGEMENT BASED ON ON‐FIELD AND CERES‐WHEAT SIMULATED DATA IN WINTER WHEAT IN THE NORTH CHINA PLAIN
Authors: Zhou Lili , Liao Shuhua , Wang Zhimin , Wang Pu , Yan Haijun , Zhang Yinghua , Zhang Li , Zhao Xue , Lin Shan , Zhou Shunli 
Published: June 2019

AbstractAccurate forecasting of the soil water in crop growth is crucial for developing an irrigation management strategy and saving water resources. The CERES model can be used to quantitatively predict the soil water content (SWC), but its accuracy and stability should be improved. The objectives of this research were to assess the CERES‐Wheat model for its ability to simulate the SWC in the winter wheat growth season and to study the possibility of improving simulation accuracy by building a modified model through introducing on‐field data and simulated values of CERES‐Wheat. Field experiment data of the varieties ShiJiaZhuang 8 and JiMai 22 from three to four irrigation treatments conducted over 4 years were used to compare precision of the CERES‐Wheat model and the modified model for SWC in the North China Plain. On average across all soil layers between 20 and 120 cm and two winter wheat varieties, the NRMSE decreased from 20.3% with the CERES‐Wheat model to 13.2% with the modified model. The results showed that the further improvement of SWC based on on‐field data and simulated data of CERES‐Wheat may be possible. Application prospects of dynamic water management of the modified model by introducing on‐field data during the wheat growth season were also discussed. © 2019 John Wiley & Sons, Ltd.
Gene and protein delivery using four cell penetrating peptides for HIV‐1 vaccine development
Authors: Rostami Bahareh , Irani Shiva , Bolhassani Azam , Cohan Reza Ahangari 
Published: June 2019

AbstractCell penetrating peptides (CPPs) can potently transport therapeutic molecules to target cells for treatment of a variety of diseases. Thus, their use is critical to improve therapeutic vaccines. Histidine‐rich nona‐arginine (HR9) and primary amphipathic peptide (MPG) showed the ability to transfer DNA into the cells. Moreover, the peptide derived from the C‐terminal of the tumor suppressor protein p14ARF (M918) and arginine‐rich peptide (penetratin) were utilized to deliver polypeptides and proteins into the living cells. In this study, the immunostimulatory properties of HIV‐1 Nef DNA and protein constructs were evaluated using small heat shock protein 20 (sHsp20) and Freund's emulsion as an adjuvant, and four CPPs (HR9, MPG, M918, and penetratin) as a gene or protein carrier in BALB/c mice. Our data indicated that the HR9/DNA, MPG/DNA, M918/protein, and penetratin/protein complexes formed the stable nanoparticles that were effectively delivered in HEK‐293T cell line at certain ratios. Moreover, a heterologous Hsp20‐Nef DNA + MPG prime/rHsp20‐Nef protein+M918 boost regimen significantly elicited higher levels of IgG2a, IgG2b, IFN‐gamma, and Granzyme B directed toward Th1 responses in a long period (3 months) after the last immunization compared to other groups. Furthermore, the effective role of Hsp20 was detected as a natural adjuvant in enhancing immune responses against HIV‐1 Nef antigen. These findings demonstrated that the simultaneous use of M918 and MPG CPPs as protein and gene carriers improves HIV‐1 Nef‐specific B‐ and T‐cell immune responses as a promising approach for development of HIV‐1 monovalent vaccine.
Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study
Authors: Zupin Luisa , Ottaviani Giulia , Rupel Katia , Biasotto Matteo , Zacchigna Serena , Crovella Sergio , Celsi Fulvio 
Published: June 2019

AbstractLaser therapy, also known as Photobiomodulation (PBM) is indicated to reduce pain associated with different pathologies and applied using protocols that vary in wavelength, irradiance and fluence. Its mechanisms of action are still unclear and possibly able to directly impact on pain transmission, reducing nociceptor response. In our study, we examined the effect of two specific laser wavelengths, 800 and 970 nm, extensively applied in the clinical context and known to exert important analgesic effects. Our results point to mitochondria as the primary target of laser light in isolated dorsal root ganglion (DRG) neurons, reducing adenosine triphosphate content and increasing reactive oxygen species levels. Specifically, the 800 nm laser wavelength induced mitochondrial dysregulation, that is, increased superoxide generation and mitochondrial membrane potential. When DRG neurons were firstly illuminated by the different laser protocols and then stimulated with the natural transient receptor potential cation channel subfamily V member 1 (TRPV1) ligand capsaicin, only the 970 nm wavelength reduced the calcium response, in both amplitude and frequency. Consistent results were obtained in vivo in mice, by subcutaneous injection of capsaicin. Our findings demonstrate that the effect of PBM depends on the wavelength used, with 800 nm light mainly acting on mitochondrial metabolism and 970 nm light on nociceptive signal transmission.
Urolithin B suppresses tumor growth in hepatocellular carcinoma through inducing the inactivation of Wnt/β‐catenin signaling
Authors: Lv Min‐yi , Shi Chuan‐jian , Pan Fei‐fei , Shao Jiang , Feng Lu , Chen Guoqin , Ou Caiwen , Zhang Jin‐fang , Fu Wei‐ming 
Published: June 2019

AbstractConsumption of dietary ellagitannins (ETs) has been proven to benefit multiple chronic health disorders including cancers and cardiovascular diseases. Urolithins, gut microbiota metabolites derived from ETs, are considered as the molecules responsible for these health effects. Previous studies have demonstrated that urolithins exhibit antiproliferative effects on prostate, breast, and colon cancers. However, as for hepatocellular carcinoma (HCC), it remains elusive. Herein, we aim to investigate the function of urolithin B (UB), a member of urolithins family, in HCC. The effects of UB on cell viability, cell cycle and apoptosis were evaluated in HCC cells, and we found UB could inhibit the proliferation of HCC cells, which resulted from cell cycle arrest and apoptosis. Furthermore, UB could increase phosphorylated β‐catenin expression and block its translocation from nuclear to cytoplasm, thus inducing the inactivation of Wnt/β‐catenin signaling. Using a xenograft mice model, UB was found to suppress tumor growth in vivo. In conclusion, our data demonstrated that UB could inhibit the proliferation of HCC cells in vitro and in vivo via inactivating Wnt/β‐catenin signaling, suggesting UB could be a promising candidate in the development of anticancer drugs targeting HCC.
Comparison of performance characteristics between high‐performance liquid chromatography and latex agglutination turbidimetric immunoassay for therapeutic drug monitoring of zonisamide
Authors: Eto Daiki , Tanaka Ryota , Suzuki Yosuke , Sato Yuhki , Itoh Hiroki 
Published: June 2019

AbstractBackgroundRecently, the Nanopia® TDM Zonisamide reagent using the latex particle‐enhanced turbidimetric immunoassay (LTIA) method was developed. The aim of this study was to compare the differences in serum zonisamide (ZNS) concentrations quantified by the high‐performance liquid chromatography (HPLC) method and the LTIA method using a TBA‐25FR analyzer.MethodsA total of 78 samples from 33 patients were quantified by both HPLC and LTIA methods. Deproteinization was used as pretreatment for the HPLC method. The ZNS concentrations quantified by two methods were compared.ResultsThe HPLC method had intra‐ and inter‐day precision lower than 1.86% and 9.00%, and accuracy better than 2.44% and 6.33%, respectively. The LTIA method showed intra‐ and inter‐day precision lower than 2.50% and 5.20%, and accuracy better than 15.80% and 10.60%, respectively. The lower limits of quantification for the HPLC and LTIA methods were 1.0 and 5.0 µg/mL, respectively. The ZNS concentration quantified by the HPLC method correlated strongly with that by the LTIA method (r = 0.953, P < 0.001). A Bland‐Altman plot suggested no systematic error between ZNS concentrations quantified by HPLC and LTIA methods.ConclusionThis study confirmed no differences between the concentrations quantified by the HPLC and LTIA methods at both high and low concentrations, demonstrating the confidence of measurement by the LTIA method.
A comparison study between wide‐range and high‐sensitivity C‐reactive protein assays (Roche Cobas c702) for low C‐reactive protein concentration in patients with cardiovascular risk
Authors: Li Runqing , Xue Yajun , Wang Tengjiao , Gong Lijun , Peng Peng , Xiong Pan , Dai Mingzhu , Shao Tongju , Hu Yuhan , Ye Xinchen 
Published: June 2019

AbstractBackgroundLow concentration C‐reactive protein (CRP) has favorable prognostic significance in patients with cardiovascular risks.MethodsWe compared the wr‐CRP method with the hs‐CRP method both on Roche Cobas c702 analyzer for the determination of low CRP concentration (<20 mg/L) including 200 patients treated in Cardiology Department in Beijing Tsinghua Changgung Hospital (Beijing, China) from December 2018 to March 2019.ResultsThe two methods were highly correlated (Spearman's rho = 0.995). Deming regression was used to fit the regression analysis model, giving a slope of 1.058 with an intercept of 0.008. The median method difference (wr‐CRP − hr‐CRP) was 0.120 mg/L (95% CI, 0.086‐0.200 mg/L), and the median percent differences were 7.34% (95% CI, 4.27%‐8.47%). The percent bias between both methods at the given cutoff CRP values of 1, 3, and 10 mg/L evaluated by Deming regression was 6.60%, 6.07%, and 5.88%, respectively, all of which were less than the acceptable standard (12.50%). The percentage of sample results concordant by both methods for the risk stratification was 96.0% (kappa = 0.937, P < 0.001).ConclusionsRoche wr‐CRP and hs‐CRP assays are highly concordant in determining low concentration CRP. Wr‐CRP may be used as an alternative to hs‐CRP assay on Roche Cobas c702 analyzer to assess the cardiovascular risk, considering its convenience and lower costs.
Potential prognostic factors for predicting the chemotherapeutic outcomes and prognosis of patients with metastatic colorectal cancer
Authors: Zhang Liqun , Zhang Jingdong , Wang Yuanhe , Dong Qian , Piao Haiyan , Wang Qiwei , Zhou Yang , Ding Yang 
Published: June 2019

AbstractObjectivesThe purpose of this study was to explore whether pretreatment potential prognostic factors are related to chemotherapeutic outcomes and the prognosis of inpatients with metastatic colorectal cancer (mCRC) undergoing chemotherapy.Materials and methodsData from 71 patients with mCRC were analyzed retrospectively. The relationship between the potential prognostic factors before first‐line chemotherapy and the clinicopathological characteristics and chemotherapy response of the patients was calculated using Fisher's exact test and the chi‐square test. The prognostic factors were analyzed using univariate and multivariate analyses. We analyzed the subgroups using the Mann‐Whitney U test.ResultsFour factors were eventually used as prognostic factors, namely the albumin‐to‐globulin ratio (AGR), the fibrinogen‐to‐albumin ratio (FAR), the prealbumin‐to‐globulin ratio (PGR), and the fibrinogen‐to‐prealbumin ratio (FPR); the cutoff values of the four potential prognostic factors were 1.40, 10.63, 5.44, and 18.49, respectively. The high AGR and PGR groups had a higher response rate than that of the low groups. Patients in the low FAR and FPR groups showed a higher objective response rate than the high FAR and FPR groups. Patients with low FPR were associated with a higher disease control rate than patients with high FPR. Higher progression‐free survival (PFS) was observed in the high AGR and PGR and low FAR and FPR groups. The AGR, FAR, PGR, and FPR were considered reliable prognostic factors for PFS in a univariate analysis.ConclusionsThe prechemotherapy AGR, FAR, PGR, and FPR were good prognostic factors to predict the chemotherapy response and PFS in patients with mCRC.
Chronic myeloid leukemia blast crisis presented with AML of t(9;22) and t(3;14) mimicking acute lymphocytic leukemia
Authors: Liu Keyu , Hu Jintian , Wang Xueqin , Li Li 
Published: June 2019

AbstractBackgroundClinically, 90%‐95% of cases of CML have the characteristic t(9;22) (q34.1;q11.2) translocation that leads to the Philadelphia (Ph) chromosome. Rarely, patients with CML can present directly in a blast crisis (BC). While most blast crises are of myeloid origin, myeloid BC with ALL‐like morphologic features and Ph‐positive acute myeloid leukemia (AML) is rare, especially at the time of CML diagnosis.Case presentationA 20‐year‐old man presented with Ph chromosome‐positive AML mimicking acute lymphocytic leukemia (ALL). Bone marrow (BM) aspiration revealed AML with ALL‐like morphologic features. The results of the immunophenotypic analysis suggested AML. Cytogenetic analysis of the BM cells revealed a 46,XY,t(3;14)(q21;q32),t(9;22)(q34;q11.2)[20] karyotype. Thus, we called the condition AML mimicking ALL. The patient was diagnosed with myeloid BC based on the combination of clinical, cytologic, and cytogenetic studies.ConclusionTo date, no case reports of a patient diagnosed with CML BC presented with Ph chromosome‐positive AML mimicking ALL have been reported. We present the case given its rarity, easy misdiagnosis, and poor prognosis. It is important to combine clinical, cytologic, and cytogenetic analyses in distinguishing CML BC from de novo AML with the t(9;22)，and further cases should be accumulated to explore how to improve the prognosis of the patients.
Acetaminophen adsorption using a low‐cost adsorbent prepared from modified residues of Moringa oleifera Lam. seed husks
Authors: Quesada Heloise B , Cusioli Luís Fernando , de O Bezerra Charleston , Baptista Aline TA , Nishi Leticia , Gomes Raquel G , Bergamasco Rosângela 
Published: June 2019

AbstractBackgroundThe high consumption of acetaminophen leads to high concentrations in surface water causing impacts on aquatic organisms, which is highlighting the need for investigation of alternative water and wastewater treatments. Therefore, the present study objective was to evaluate the adsorption capacity of acetaminophen onto Moringa oleifera Lam. seed husks after chemical and thermal treatment.ResultsThe characterization of the adsorbent revelaed heterogeneous morphology with the presence of micro‐ and mesopores. Adsorption studies showed that the adsorption did not altered between pH 3 and 9, and that there was no significant effect of the ionic strength, indicating that the mechanism does not involve electrostatic interactions. In fact, Fourier transform infrard spectra showed that acetaminophen was adsorbed by hydrogen bonds and π‐stacking. The kinetic data were adjusted to the pseudo‐first‐order model, indicating an equilibrium time of 18 h. The equilibrium study showed that adsorption occurred in a monolayer and was favoured by the increase in temperature. The maximum adsorption capacity was 17.48 mg g−1, at 318 K, higher than some capacities found in the literature. The thermodynamic parameters indicated that the process was spontaneous, endothermic and physical.ConclusionsThe characteristics and results found compared to the recent literature verified the possibility of applying this residue as a low‐cost adsorbent for the removal of acetaminophen. © 2019 Society of Chemical Industry
Management of the uncinate process via the artery first approach in laparoscopic pancreatoduodenectomy
Authors: Jiang Chong‐Yi , Liang Yun , Wang Hong‐Wei , Hu Peng‐Fei , Cai Zhi‐Wei , Wang Wei 
Published: June 2019

AbstractBackgroundUncinate process dissection is one of the major challenges for surgeons when performing laparoscopic pancreatoduodenectomy. This study aimed to evaluate the artery first approach for handling uncinate process dissection in laparoscopic pancreatoduodenectomy.MethodsBetween February 2015 and June 2018, a total of 91 consecutive patients without vascular encasement underwent selective laparoscopic pancreatoduodenectomy, including the first 26 consecutive cases treated with the conventional approach and the remaining 65 with the artery first approach applied for uncinate process dissection. Here, we present and analyze the surgical outcomes and the oncological results for the two groups.ResultsThere was no significant difference between the two groups in operative time, intraoperative blood loss, the rate of conversion to open pancreatoduodenectomy, postoperative complications, mortality, as well as the number of lymph nodes retrieved in the malignancies. In contrast, the artery first approach group showed a statistically significant shorter resection time and a higher R0 resection rate when compared with the conventional group.ConclusionsThe artery first approach is a safe and feasible technique that can be used for uncinate process management in laparoscopic pancreatoduodenectomy for patients without vascular encasement. It also has the advantage of increased rate of radical resection in the surgical intervention of relevant malignancies.
Magnon mode in α‐MnS Raman spectrum
Authors: Gerasimova Julia V. , Abramova Galina M. , Zhandun Vyacheslav S. , Vtyurin Aleksandr N. 
Published: June 2019

AbstractRaman spectra of manganese monosulphide (α‐MnS) with NaCl structure in the temperature range 5–300 К are presented. A new peak at 22 cm–1 has been found at low temperatures. The temperature dependence of this peak made possible to attribute it to the magnon mode at k = 0. This is in good agreement with the magnon wavenumber calculated with Munich SPRKKR‐package.
Wideband balun filtering quasi‐Yagi antenna with high selectivity
Authors: Song Lei , Wu Bian , Xu Mei , Su Tao , Lin Lei 
Published: June 2019

AbstractA compact wideband balun filtering quasi‐Yagi antenna with high selectivity is proposed. It consists of a quasi‐Yagi antenna integrated with a multilayer balun filter. The quasi‐Yagi antenna is composed of a bow‐tie driver coupled with a director on the top layer, and a ground reflector on the bottom layer. The fourth‐order balun filter has two stepped impedance resonators (SIRs) on the top layer, and two SIRs on the middle layer, which helps improve the frequency selectivity and bandwidth. Moreover, it has two differential ports to feed the quasi‐Yagi antenna. The filtering antenna centered at 2.5 GHz with a wide bandwidth of 22.8% and a pair of transmission zeros is designed and fabricated, which shows a stable radiation pattern with maximum gain of 5.5 dBi and high selectivity.
Magnetically reconfigurable pixelated antenna
Authors: Pal Jitendra , Donzelli Francesco , Piazza Daniele , Bain James A. , Piazza Gianluca 
Published: June 2019

AbstractIn this article, a total of 70 movable nickel (Ni) patches are monolithically integrated with fixed copper (Cu) elements to form a reconfigurable patch antenna. The Ni patches are magnetically actuated by means of an array of permanent magnets (PM) placed behind the ground plane of the antenna. The Ni patches establish low resistance mechanical contact between two fixed Cu patches due to the high contact force generated by the PM. Since the Ni patch is itself part of the radiating element, this approach eliminates the need to insert an external switch on the antenna plane. The PMs, which could be replaced by electronically controlled PMs, are placed behind the ground plane, therefore eliminating losses associated with bias lines generally used in pixelated antennas. The 70 movable element pixelated antenna is capable of reconfiguration in frequency, bandwidth, polarization, and beam steering. Herein, we demonstrate frequency and bandwidth reconfiguration.