bluuebunny/crossref_metadata_2025_split huggingface dataset:
β1D integrin splice variant stabilizes integrin dynamics and reduces integrin signaling by limiting paxillin recruitment
Authors: Soto-Ribeiro Martinho , Kastberger Birgit , Bachmann Michael , Azizi Latifeh , Fouad Kenza , Jacquier Marie-Claude , Boettiger David , Bouvard Daniel , Bastmeyer Martin , Hytönen Vesa P. , Wehrle-Haller Bernhard 
Published: March 2019

ABSTRACT
Heterodimeric integrin receptors control cell adhesion, migration and extracellular matrix assembly. While the α integrin subunit determines extracellular ligand specificity, the β integrin chain binds to an acidic residue of the ligand, and cytoplasmic adapter protein families such as talins, kindlins and paxillin, to form mechanosensing cell matrix adhesions. Alternative splicing of the β1 integrin cytoplasmic tail creates ubiquitously expressed β1A, and the heart and skeletal muscle-specific β1D form. To study the physiological difference between these forms, we developed fluorescent β1 integrins and analyzed their dynamics, localization, and cytoplasmic adapter recruitment and effects on cell proliferation. On fibronectin, GFP-tagged β1A integrin showed dynamic exchange in peripheral focal adhesions, and long, central fibrillar adhesions. In contrast, GFP–β1D integrins exchanged slowly, forming immobile and short central adhesions. While adhesion recruitment of GFP–β1A integrin was sensitive to C-terminal tail mutagenesis, GFP–β1D integrin was recruited independently of the distal NPXY motif. In addition, a P786A mutation in the proximal, talin-binding NPXY783 motif switched β1D to a highly dynamic integrin. In contrast, the inverse A786P mutation in β1A integrin interfered with paxillin recruitment and proliferation. Thus, differential β1 integrin splicing controls integrin-dependent adhesion signaling, to adapt to the specific physiological needs of differentiated muscle cells.
Genome-wide identification of alternative splicing events that regulate protein transport across the secretory pathway
Authors: Neumann Alexander , Schindler Magdalena , Olofsson Didrik , Wilhelmi Ilka , Schürmann Annette , Heyd Florian 
Published: March 2019

ABSTRACT
Alternative splicing (AS) strongly increases proteome diversity and functionality in eukaryotic cells. Protein secretion is a tightly controlled process, especially when it occurs in a tissue-specific and differentiation-dependent manner. While previous work has focussed on transcriptional and post-translational regulatory mechanisms, the impact of AS on the secretory pathway remains largely unexplored. Here, we integrate results from a published screen for modulators of protein transport and RNA-Seq analyses to identify over 200 AS events as secretion regulators. We confirm that splicing events along all stages of the secretory pathway regulate the efficiency of membrane trafficking using morpholino and CRISPR/Cas9 experiments. We furthermore show that these events are highly tissue-specific and mediate an adaptation of the secretory pathway during T-cell activation and adipocyte differentiation. Our data substantially advance the understanding of AS functionality, add a new regulatory layer to a fundamental cell biological process and provide a resource of alternative isoforms that control the secretory pathway.
Typing complex meningococcal vaccines to understand diversity and population structure of key vaccine antigens
Authors: Rodrigues Charlene M. C. , Chan Hannah , Vipond Caroline , Jolley Keith , Harrison Odile B. , Wheeler Jun , Whiting Gail , Feavers Ian M. , Maiden Martin C. J. 
Published: March 2019

Background:Protein-conjugate capsular polysaccharide vaccines can potentially control invasive meningococcal disease (IMD) caused by five (A, C, W, X, Y) of the six IMD-associated serogroups. Concerns raised by immunological similarity of the serogroup B capsule to human neural cell carbohydrates, meant that ‘serogroup B substitute’ vaccines target more variable subcapsular protein antigens. A successful approach using outer membrane vesicles (OMVs) as major vaccine components had limited strain coverage. In 4CMenB (Bexsero®), recombinant proteins have been added to ameliorate this problem. Methods: Scalable, portable, genomic techniques were used to investigate the Bexsero®OMV protein diversity in meningococcal populations. Shotgun proteomics identified 461 proteins in the OMV, defining a complex proteome. Amino acid sequences for the 24 proteins most likely to be involved in cross-protective immune responses were catalogued within thePubMLST.org/neisseriadatabase using a novel OMV peptide Typing (OMVT) scheme.Results:Among these proteins there was variation in the extent of diversity and association with meningococcal lineages, identified as clonal complexes (ccs), ranging from the most conserved peptides (FbpA, NEISp0578, and putative periplasmic protein, NEISp1063) to the most diverse (TbpA, NEISp1690). There were 1752 unique OMVTs identified amongst 2492/3506 isolates examined by whole-genome sequencing (WGS). These OMVTs were grouped into clusters (sharing ≥18 identical OMVT peptides), with 45.3% of isolates assigned to one of 27 OMVT clusters. OMVTs and OMVT clusters were strongly associated with cc, genogroup, and Bexsero®antigen variants, demonstrating that combinations of OMV proteins exist in discrete, non-overlapping combinations associated with genogroup and Bexsero®Antigen Sequence Type. This highly structured population of IMD-associated meningococci is consistent with strain structure models invoking host immune and/or metabolic selection.Conclusions:The OMVT scheme facilitates region-specific WGS investigation of meningococcal diversity and is an open-access, portable tool with applications for vaccine development, especially in the choice of antigen combinations, assessment and implementation.
Exploring the impact of glutathione S-transferase (GST)-based metabolic resistance to insecticide on vector competence of Anopheles funestus for Plasmodium falciparum
Authors: Ndo Cyrille , Kopya Edmond , Irving Helen , Wondji Charles 
Published: March 2019

Background:Malaria control heavily relies on insecticide-based interventions against mosquito vectors. However, the increasing spread of insecticide resistance is a major threat. The extent to which such resistance, notably metabolic resistance, interferes with the development of thePlasmodiumparasite and its impact on overall malaria transmission remains poorly characterized. Here, we investigated whether glutathione S-transferase-based resistance could influencePlasmodium falciparumdevelopment inAnopheles funestus.Methods:Anopheles funestusfemales were infected withP. falciparumgametocytes and midguts were dissected at day 7 post infection for detection/quantification of oocysts. Infection parameters were compared between individual with different L119F-GSTe2 genotypes, and the polymorphism of the GSTe2 gene was analyzed in infected and uninfected mosquito groups.Results:Overall, 403 mosquitoes were dissected and genotyped. The frequency of the L119F-GSTe2 resistance allele was significantly higher in non-infected (55.88%) compared to infected (40.99%) mosquitoes (Fisher's exact test, P<0.0001). Prevalence of infection was significantly higher in heterozygous and homozygous susceptible genotypes (P<0.001). However, homozygous resistant and heterozygous mosquitoes exhibited significantly higher infection intensity (P<0.01). No association was observed between the GSTe2 polymorphism and the infection status of mosquitoes.Conclusion:Altogether, these results suggest that GSTe2-based metabolic resistance may affect the vectorial competence of resistantAn. funestusmosquitoes toP. falciparuminfection, by increasing its permissiveness toPlasmodiuminfection.
ANALYSIS OF EFFECT OF FIRM SIZE, INSTITUTIONAL OWNERSHIP, PROFITABILITY, AND LEVERAGE ON FIRM VALUE WITH CORPORATE SOCIAL RESPONSIBILITY (CSR) DISCLOSURE AS INTERVENING VARIABLES (Study on Banking Companies Listed on BEI Period 2012-2016)
Authors: Astuti Fitria Yuni , Wahyudi Sugeng , Mawardi Wisnu 
Published: March 2019

The main objective of the company survives in the stringent competitive by maximizing the value of the company to shareholder wealth. Firm value is experiencing a downward trend is a problem that must be resolved. The banking company also undertakes a social responsibility that is perceived to have a positive impact on the company's image. This study aimed to analyze the effect of the firm size, institutional ownership, profitability, and leverage with corporate social responsibility (CSR) disclosure as a variable intervening.Populations are banking companies listed on the Indonesia Stock Exchange Period 2012-2016. Sampling technique used is purposive sampling with 29 companies selected according to predetermined criteria. This research is done by using multiple linear regression analysis methods and path analysis also Sobel test to examine the effect of intervening. Test results with CSRD as the dependent variable indicate that institutional ownership and Leverage have no effect on CSRD While firm size and profitability (ROA) have a positive significant effect on CSRD. The result of testing with Firm Value (Tobins'Q) as the dependent variable indicates that Firm Size, Institutional Ownership, and Profitability (ROA) have no effect on Firm Value (Tobins'Q). Leverage (DAR) has a negative and significant influence on Firm Value (Tobins'Q) while CSRD has a significant positive effect on Firm Value (Tobins'Q). CSRD does not mediate the effect of Institutional Ownership, Profitability (ROA) and Leverage (DAR) on Firm Value (Tobins'Q). CSRD mediates the effect of Firm Size on Firm Value (Tobins'Q).
Antihyperglycemic and antihyperlipidemic activities of Nannochloropsis oculata microalgae in Streptozotocin-induced diabetic rats
Authors: Nasirian Fariba , Sarir Hadi , Moradi-kor Nasroallah 
Published: March 2019

AbstractBackgroundIt is well documented that biologically active components of microalgae can be utilized for treatment of different diseases. This study was conducted to evaluate the antihyperglycemic and antihyperlipidemic activities and weight control ofNannochloropsis oculatamicroalgae (NOM) in Streptozotocin-induced diabetic male rats.MethodsDiabetes was induced by intraperitoneal administration of Streptozotocin (55 mg/kg). Healthy and diabetic rats were divided in to six groups. Healthy and diabetic rats orally received distilled water or NOM (10 and 20 mg/kg) for three weeks.ResultsOral administration of NOM to diabetic rats significantly reduced the serum concentrations of glucose, cholesterol, triglycerides, LDL and increased the serum concentration of insulin and HDL-C (P<0.05). Treatment with NOM had no significant effect on blood parameters in healthy rats (P>0.05). Also, NOM maintained body weight in diabetic rats (P<0.05).ConclusionIt can be concluded that NOM has antihyperglycemic and antihyperlipidemic activities in diabetic rats.
Narrowed reference intervals for complete blood count in a multiethnic population
Authors: Lee Seungok , Ong Chui Mei , Zhang Yu , Wu Alan H.B. 
Published: March 2019

Abstract

Background
Biological variation studies have shown that the complete blood count (CBC) has narrow within-individual variation and wide group variation, indicating that the use of reference intervals (RIs) is challenging. The aim of this study was to examine differences in CBC RIs according to race/ethnicity in a multiethnic population at a hospital in San Francisco in hopes of improving the medical utility of CBC testing.

Methods
Subject data were obtained by screening CBC results from the medical records of outpatients meeting certain criteria who visited Zuckerberg San Francisco General Hospital from April 2017 to January 2018. From these records, sex- and race/ethnicity-specific CBC RIs were calculated as the 2.5th to 97.5th percentiles.

Results
From a total of 552 subjects, 47.9% were male (65 White, 50 Black, 71 Hispanic and 54 Asian) and 52.1% were female (51 White, 39 Black, 122 Hispanic and 72 Asian). The RIs of neutrophil, lymphocyte and eosinophil counts; and hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) showed significant differences (p<0.05) among the four racial/ethnic groups: neutrophil, lymphocyte and eosinophil counts; and MCHC in males, and hemoglobin, MCV, MCH and MCHC in females.

Conclusions
Race/ethnicity-specific CBC RIs should be taken into consideration in a multiethnic population to better interpret patient status and make progress toward precision medicine.
Phytic Acid Extracted from Rice Bran as a Growth Promoter for Euglena gracilis
Authors: Zhu Jiangyu , Hong Dang Diem , Wakisaka Minato 
Published: March 2019

AbstractA significant promotion of growth and accumulation of metabolites of freshwater microalga Euglena gracilis was obtained by adding phytic acid to the culture. Phytic acid concentration of 0.05% (v/v) showed a maximum biomass increase of 1.6-fold compared to the control group. Synchronous variation in the content and proportion of photosynthetic pigments was also observed. The total chlorophyll content increased with phytic acid concentration, suggesting the enhanced capacity of absorbing light. Cell length, an important biomarker for E. gracilis, was prolonged to a certain extent under light condition, indicating the state of the cells was more active. Since phytic acid is derived from agro waste of rice bran, it is promising as a low-cost but high-efficient growth promoter of E. gracilis.
Factors associated with knowledge of where to access health care among youth in juvenile detention: a mixed methods study
Authors: Balogun Titilola , Troisi Catherine , Swartz Michael D. , Lloyd Linda , Beyda Rebecca 
Published: March 2019

Abstract

Background
Mental illness, substance use, and sexual health issues are major health concerns among detained youth compared with the general population. There is a dearth of studies that demonstrate what detained youth in the United States know about where to access health services in the community. Given the magnitude of these health concerns among detained youth compared with youth in the general population, knowing where to access health care in the community could lead to early intervention and better health outcomes.

Objectives
This study determines what detained youth know about where to access care for mental health symptoms, substance use, and sexually transmitted infections, and identifies the factors associated with knowledge of where to access health care.

Methods
Data were collected using mixed methods from a cross-sectional sample of 301 detained youth. Information was obtained using surveys (which included closed and open-ended questions), and data abstraction from their medical records. Logistic regression analysis, Chi-squared tests and thematic analysis were used to analyze the data. Results Having a primary care provider, perceived susceptibility, and previous experiences with health care providers all influenced what youth in our study knew about where to seek health care.

Conclusions
The use of mixed methods including open-ended questions allowed us to gain a better perspective of where detained youth would seek help for health issues. Detained youth have poorer health outcomes yet many did not know where to access health resources. Primary care providers who see youth should provide comprehensive, youth-friendly services.
The significant role of educational status in PKU patients: the beneficial effect of psychological support in depression
Authors: Iakovou Kostas , Schulpis Kleopatra 
Published: March 2019

Abstract

Introduction
Classical Phenylketonuria (PKU) is a metabolic disease characterized by high phenylalanine (phe) levels in blood and brain. PKU patients are commonly treated with low phe diet supplemented with amino acid free formula. High Phe levels minimize brain tryptophan concentration, the pressure of serotonin, which is responsible for the appearance of depression symptoms. Both amino acids share the same pathway for entering CNS via BBB. Aimed to determine the effect of psychological support on different education status depressed PKU patients via adherence to their diet.

Methods
PKU patients (n = 110) were divided into groups according to their education status: Primary school, High school, University degree. All patients were tasted with a Patient Health Questionnaire (PHQ-9) standardized for Greek population. Psychological support was performed in every depressed patient ones per two weeks for three successive months under the same conditions. Phe blood levels were measured before psychological support and every 15–20 days till the end of the study.

Results
Only 2/110 (1.8%) participance had finished Primary school and were healthy. 72/110 (65.5%) High School, out of them 29 were depressed and 36/110 (32.7%) achieved a University degree, only 6 suffered from depression. High phe blood levels were measured in the depressed patients, which dropped near to normal after the end of their psychological support. Referring to depression symptoms, all participance except one presented amelioration of their mood.

Conclusions
Psychological support is beneficial on depressed PKU patients graduated with High School or University degree via adherence to their special diet.
Database Creation and Dialect-Wise Comparative Analysis of Prosodic Features for Punjabi Language
Authors: Arora Shipra J. , Singh Rishipal 
Published: March 2019

Abstract
The paper represents a Punjabi corpus in the agriculture domain. There are various dialects in the Punjabi language and the main concentration is on major dialects, i.e. Majhi, Malwai and Doabi for the present study. A speech corpus of 125 isolated words is taken into consideration. These words are uttered by 100 speakers, i.e. 60 Malwi dialect speakers (30 male and 30 female), 20 Majhi dialect speakers (10 male and 10 female) and 20 Doabi dialect speakers (10 male and 10 female). Tonemes, adhak (geminated) and nasal words are selected from the corpus. Recordings have been processed through two mediums. The paper also elaborates some distinctive features of the corpus. This corpus is of quite significance for the speech recognition system. Prosodic characteristics such as intonation, rhythm and stress create a crucial impact on the speech recognition system. These characteristics vary from language to language as well as various dialects of a language. This paper portrays a comparative analysis of isolated words prosodic features of Malwi, Majhi and Doabi dialects of Punjabi language. Analysis is done using the PRAAT tool. Pitch, intensity, formant I and formant II values are extracted for toneme, adhak, nasal (bindi) and nasal (tippi) words. For all kinds of words, there is a significant variation in pitch (fundamental frequency), intensity, formant I and formant II values of male and female speakers of Malwi, Majhi and Doabi dialects. A detailed analysis has been discussed throughout this paper.
Managing the Learning Capacity of Organizational Culture in Relation to Organizational Commitment: Methodological and Empirical Overview
Authors: Nakov Leonid , Ivanovski Igor 
Published: March 2019

Abstract
The primary objective of the paper is to offer qualitative analysis based on the previous relevant theoretical and empirical work for the correlation between systematic and methodological implementation of organizational commitment throughout its learning capacity and organizational culture. The co-integration of the two critical issues stresses the significance and influence of contemporary and holistic models, such as, the OCTA-PACE model, and its convergence to High Commitment management Model (HCM), as highly important for organizational growth and competitiveness of business entities.
Crystal structure of (4,4′-(ethane-1,2-diylbis((nitrilo)(2-furylmethylylidene)))bis(3-methyl-1-phenyl-1H-pyrazol-5-olato-κ4
N,N′,O,O′))-nickel(II)), C32H26N6NiO4
Authors: Wei Wei , Wu Qiong , Zhang Heng-Qiang , Jin Tong-Yin , Chen Hong-Li 
Published: March 2019

Abstract
C32H26N6NiO4, monoclinic, C2/c (no. 15), a = 22.270(4) Å, b = 16.814(2) Å, c = 8.1486(9) Å, β = 111.222(3)°, V = 2835.0(9) Å3, Z = 4, R
gt(F) = 0.0481, wR
ref(F
2) = 0.1440, T = 295(2) K.
„Seltenheitswert in der Zeit“
Authors: Kirchhoff Christine 
Published: March 2019

AbstractFreuds Arbeit überVergänglichkeitwird in den Kontext der Auseinandersetzung mit Endlichkeit und Sterblichkeit gestellt, wie sie in Literatur und bildender Kunst bis in die Gegenwart hinein geführt wird. Dabei zeigt sich, dass die Spannung zwischencarpe diemundmemento moriin Freuds Text als Spannung zwischen manischer Verleugnung und depressiver Reaktion – also in der Sprache der Psychoanalyse – wieder auftaucht. Es wird gefragt, inwiefern der leicht manische Einschlag, den Freuds Verteidigung des Genusses des Schönen angesichts der Vergänglichkeit annimmt, auch dem Umstand geschuldet sein könnte, dass er sich dem Thema Verlust, insbesondere der sicher geglaubten kulturellen Errungenschaften, nicht aus einer Perspektive der Theorie der Objektbeziehungen nähert. Mit Bezug auf Roger Money-Kyrle lässt sich zeigen, dass auf diese Weise ein Weg eröffnet wird, dem Ringen um Genuss und Freude angesichts der Vergänglichkeit, die zum einen in der verstreichenden Zeit und zum anderen in der menschlichen Destruktivität begründet liegt, konzeptionell eine andere Wendung zu geben. Ferner wird die Vergänglichkeit zusammen mit dem psychoanalytischen Konzept der Nachträglichkeit diskutiert. Hier rückt die spezifische Zeitlichkeit in den Fokus und es eröffnet sich eine neue Perspektive, die für eine kurze Reflexion der Popularität von Vanitas-Topoi in der Gegenwartskunst genutzt wird.
Attitudes of Students from the High Medical College of Professional Studies and Nurses Towards People Suffering from Dementia
Authors: Stanic Gordana , Opancina Valentina , Rancic Nemanja , Jovic Jelena , Ignjatovic-Ristic Dragana 
Published: March 2019

Abstract
Dementia is characterized by a progressive decrease in cognitive functions, and the term includes different etiologies. Cognitive decline includes loss of memory and deterioration in executive functions, such as planning and organizing skills, sufficient to influence social activities. The aim of this study was to examine and compare the attitudes (knowledge, emotions and behaviour) of students at the High Medical College of Professional Studies and nurses towards people suffering from dementia. The study was designed as a qualitative study with the use of a questionnaire. The Dementia Attitudes Scale (DAS) was used in our study. A total of 283 respondents answered the survey: 56.25% were students, and 43.75% were nurses. The internal consistency of the DAS was found to be good with a Cronbach’s α of 0.792. In the overall score for attitudes, a significant difference was found between students (100.47±10.91) and nurses (95.51±16.10). The students had a better score regarding questions describing their behaviour towards these individuals (p<0.001) and emotions for these patients (p<0.001). For knowledge, there was no difference between the two groups of subjects (p=0.901). Regarding the overall score, attitudes of students and nurses towards people with dementia were positive. This research suggested that the training of senior team members who then had dementia expertise was a key component in developing attitudes and improving care practices and outcomes for these patients. Continuous education of all medical staff who have contact with people who suffer from dementia is important.