bluuebunny/crossref_metadata_2025_split huggingface dataset:
Formation of organotypic testicular organoids in microwell culture†
Authors: Sakib Sadman , Uchida Aya , Valenzuela-Leon Paula , Yu Yang , Valli-Pulaski Hanna , Orwig Kyle , Ungrin Mark , Dobrinski Ina 
Published: March 2019

AbstractThree-dimensional (3D) organoids can serve as an in vitro platform to study cell–cell interactions, tissue development, and toxicology. Development of organoids with tissue architecture similar to testis in vivo has remained a challenge. Here, we present a microwell aggregation approach to establish multicellular 3D testicular organoids from pig, mouse, macaque, and human. The organoids consist of germ cells, Sertoli cells, Leydig cells, and peritubular myoid cells forming a distinct seminiferous epithelium and interstitial compartment separated by a basement membrane. Sertoli cells in the organoids express tight junction proteins claudin 11 and occludin. Germ cells in organoids showed an attenuated response to retinoic acid compared to germ cells in 2D culture indicating that the tissue architecture of the organoid modulates response to retinoic acid similar to in vivo. Germ cells maintaining physiological cell–cell interactions in organoids also had lower levels of autophagy indicating lower levels of cellular stress. When organoids were treated with mono(2-ethylhexyl) phthalate (MEHP), levels of germ cell autophagy increased in a dose-dependent manner, indicating the utility of the organoids for toxicity screening. Ablation of primary cilia on testicular somatic cells inhibited the formation of organoids demonstrating an application to screen for factors affecting testicular morphogenesis. Organoids can be generated from cryopreserved testis cells and preserved by vitrification. Taken together, the testicular organoid system recapitulates the 3D organization of the mammalian testis and provides an in vitro platform for studying germ cell function, testicular development, and drug toxicity in a cellular context representative of the testis in vivo.
Protective Effect of Biculturalism for Health Amongst Minority Youth: The Case of Pacific Islander Migrant Youths in Hawai’i
Authors: Lee Hye-ryeon , Lee Hye Eun , Cassel Kevin , Hagiwara Megan Inada , Somera Lilnabeth P 
Published: March 2019

Abstract
This study investigates the mechanisms by which biculturalism impacts various health outcomes amongst youth migrants to Hawai’i who are from the US-Affiliated Pacific Islands jurisdictions. Using purposive sampling, 284 males and females (twelve to nineteen years old) of Pacific Islander ethnicities in Hawai’i completed a survey. Results from path analysis showed that biculturalism significantly and positively affected self-esteem that, in turn, improved eating attitude, body satisfaction and perceived well-being. Further, eating attitude increased healthy eating behaviour and body satisfaction that, in turn, positively affected general health perception and body satisfaction. Positive smoking attitudes increased smoking activities, which negatively affect general health perception. The study demonstrated that self-esteem impacted overall health through its influence on enhancing positive perceptions about the importance of healthy eating, body satisfaction and well-being. Biculturalism indirectly led to increased self-esteem, which in turn directly influenced attitudes about healthy eating, body satisfaction and perceived well-being. Our study provides strong evidence that addressing the problem of health disparities for minority populations in the USA has to start with reaffirming the value of diversity and multiculturalism and embracing an individual’s historic cultural identity. Specific implications for funding agencies and researchers of minority health programs are discussed.
Socio-Economic, Psychosocial, Lifestyle and Community Factors do not Explain Ethnic Inequalities in Obesity in Disadvantaged Israeli Mothers
Authors: Soskolne Varda , Cohen-Dar Michal , Obeid Samira , Cohen Nitsa , Rudolf Mary C J 
Published: March 2019

AbstractGuided by the psycho-social environment approach to explaining health inequalities, this cross-sectional study aimed to assess the contribution of individual and community factors to explaining ethnic inequalities in overweight and obesity between Arab and Jewish mothers of young children in Israel. Data (N = 946, 371 Jewish, 575 Arab mothers) were collected by self-report questionnaire in mid-2015 as part of a special ‘Preparation for School’ project for children aged 5–6 years from twenty Mother and Child Health clinics in towns and villages of lowest socio-economic ranking in northern Israel. Multinomial logistic regression models were conducted to assess the effect of socio-economic status (SES), psychological, lifestyle behaviour and community food-related practices on mediating the association of ethnicity with overweight and obesity. Overweight and obesity were significantly higher among Arab mothers. The strength of the association of ethnicity with overweight [(odds ratio) OR = 1.80, 95 per cent confidence interval (CI) = 1.31, 2.47] or obesity (OR = 2.14. 95 per cent CI = 1.44, 3.18) remained constant after SES, and other variables were included in two steps. The persistence of ethnic inequalities suggests that the variables included in the analysis did not explain inequalities in this disadvantaged population. Social work may contribute to understanding additional explanatory variables that have the potential to be amenable to change by multidisciplinary and social work interventions.
Arteriovenous fistula thrombosis is associated with increased all-cause and cardiovascular mortality in haemodialysis patients from the AURORA trial
Authors: Girerd Sophie , Girerd Nicolas , Frimat Luc , Holdaas Hallvard , Jardine Alan G , Schmieder Roland E , Fellström Bengt , Settembre Nicla , Malikov Sergei , Rossignol Patrick , Zannad Faiez 
Published: March 2019

Abstract

Background
The impact of arteriovenous fistula (AVF) or graft (AVG) thrombosis on mortality has been sparsely studied. This study investigated the association between AVF/AVG thrombosis and all-cause and cardiovascular mortality.

Methods
The data from 2439 patients with AVF or AVG undergoing maintenance haemodialysis (HD) included in the A Study to Evaluate the Use of Rosuvastatin in Subjects on Regular Hemodialysis: An Assessment of Survival and Cardiovascular Events trial (AURORA) were analysed using a time-dependent Cox model. The incidence of vascular access (VA) thrombosis was a pre-specified secondary outcome.

Results
During follow-up, 278 AVF and 94 AVG thromboses were documented. VA was restored at 22 ± 64 days after thrombosis (27 patients had no restoration with subsequent permanent central catheter). In multivariable survival analysis adjusted for potential confounders, the occurrence of AVF/AVG thrombosis was associated with increased early and late all-cause mortality, with a more pronounced association with early all-cause mortality {hazard ratio [HR] &lt; 90 days 2.70 [95% confidence interval (CI) 1.83–3.97], P &lt; 0.001; HR &gt; 90 days 1.47 [1.20–1.80], P &lt; 0.001}. In addition, the occurrence of AVF thrombosis was significantly associated with higher all-cause mortality, whether VA was restored within 7 days [HR 1.34 (95% CI 1.02–1.75), P = 0.036] or later than 7 days [HR 1.81 (95% CI 1.29–2.53), P = 0.001].

Conclusions
AVF/AVG thrombosis should be considered as a major clinical event since it is strongly associated with increased mortality in patients on maintenance HD, especially in the first 90 days after the event and when access restoration occurs &gt;7 days after thrombosis. Clinicians should pay particular attention to the timing of VA restoration and the management of these patients during this high-risk period. The potential benefit of targeting overall patient risk with more aggressive treatment after AVF/AVG restoration should be further explored.
Lowering Stand Density Enhances Resiliency of Ponderosa Pine Forests to Disturbances and Climate Change
Authors: Zhang Jianwei , Finley Kaelyn A , Johnson Nels G , Ritchie Martin W 
Published: March 2019

AbstractStand density affects not only structure and growth, but also the health of forests and, subsequently, the functions of forest ecosystems. Here, we integrated dendrochronology and repeated inventories for ponderosa pine research plots to determine whether long-term growth and mortality responded to climate trends and how varying stand density influenced the responses. The plots were established prior to 1975 on existing stands throughout northern California. Although annual temperature increased consistently for the last 65 years, ring-width indices produced by eliminating age and thinning effects failed to detect radial trend regardless of site quality. However, interannual variation for the indices was substantial, reflecting a strong influence of climate on tree growth. Plot-level basal area increments were significantly affected by tree mortality. Stand density index explained most variation of mortality. Lowering stand density enhanced remaining tree growth, reduced mortality, and increased stand resiliency to disturbances and climate change. Besides higher climate moisture indices or lower vapor pressure deficits, any treatments that improve tree vigor and reduce stress will have a similar effect to reducing stand density. Although neither biotic disturbances nor abiotic conditions can be controlled, forest managers can manage stand density appropriately to enhance resilience to climate change and disturbances.
Cost and Productivity of Tethered Cut-to-Length Systems in a Dry-Forest Fuel-Reduction Treatment: A Case Study
Authors: Petitmermet Joshua , Sessions John , Bailey John , Zamora-Cristales Rene 
Published: March 2019

AbstractFuel-reduction treatments on steep slopes across federal forests of the western United States have been limited by the high costs associated with cable logging on steeper slopes combined with poor market prospects for small-diameter material (Bolding 2003, Rummer 2008, Han et al. 2016). The emergence of tethered cut-to-length harvesting systems and small wood markets (e.g., biochar) could decrease costs and increase revenue generated from treatments. Over the course of 3 weeks, we observed both tethered (steeper slopes) and untethered cut-to-length fuel-reduction treatment on the Fremont-Winema National Forest in south-central Oregon and interviewed operators. We used those data to derive and contrast hourly costs and productivity for the harvester and forwarder. This was the first time a tethered harvester and forwarder were used in a fuel-reduction treatment on federal forests in this region. We developed and tested a variety of work time model forms for each machine. The mean utilization rate for the harvester was 64 percent on 17 tethered consolidated corridors but 87 percent on 28 untethered consolidated corridors. Similarly, the forwarder had a mean utilization rate of 76 percent on 30 tethered trips and 89 percent on 114 untethered trips. This reduced utilization rate could be because of the direct effects of tethering, the increased complexities of operations associated with steeper slopes, and the stand characteristics (e.g., lower stand density and tree sweep) associated with steeper slopes. Costs during tethered operations were higher than during nontethered operations, but lower than previous reports using cable logging.
High multi-azole-resistant Malassezia pachydermatis clinical isolates from canine Malassezia dermatitis
Authors: Kano Rui , Aramaki Chizu , Murayama Nobuo , Mori Yuko , Yamagishi Kentaro , Yokoi Shinichi , Kamata Hiroshi 
Published: March 2019

Abstract
Malassezia pachydermatis, a lipophilic and aerobic yeast, is a causative agent of Malassezia dermatitis, a common skin mycosis in dogs and cats. This fungus is also responsible for zoonotic fungal infections in human neonates. Ravuconazole (RVZ) is an antifungal azole compound and the active metabolite of fosravuconazole, which was approved for use in humans in Japan in 2018. In the present study, in vitro RVZ susceptibility and multi-azole resistance of 13 clinical M. pachydermatis strains was investigated using the modified Clinical and Laboratory Standards Institute M27-A3 test. The minimum inhibitory concentrations (MICs) for the 13 isolates ranged from 0.094 to >32 mg/L for itraconazole (ITZ) and from 0.5 to >32 mg/l for RVZ. Similarly, MICs for ITZ- or RVZ-resistant strains (MICs >32 mg/l) were also >32 mg/l for clotrimazole (CTZ), >32 mg/l for miconazole (MCZ), and 0.25 to >32 mg/L for voriconazole (VRZ). BLAST analysis using the NCBI database showed that ERG11 cDNA of the RVZ-resistant strain encoded Gly at codon 461 and Asp in cytochrome p450 encoded by M. pachydermatis ERG11 mRNA. This work is the first report to describe that an RVZ-resistant M. pachydermatis strain contains ERG11 mutations. The affinity of the protein encoded by ERG11 for RVZ may differ from that of ITZ. Therefore, RVZ has considerable therapeutic potential for treating ITZ-resistant canine Malassezia dermatitis. However, RVZ-resistant strains already exist in canine Malassezia dermatitis in Japan.
A stochastic propagation model to the energy dependent rapid temporal behaviour of Cygnus X-1 as observed by AstroSat in the hard state
Authors: Maqbool Bari , Mudambi Sneha Prakash , Misra R , Yadav J S , Gudennavar S B , Bubbly S G , Rao A , Jogadand S , Patil M K , Bhattacharyya S , Singh K P 
Published: March 2019

Abstract
We report the results from analysis of six observations of Cygnus X-1 by Large Area X-ray Proportional Counter (LAXPC) and Soft X-ray Telescope (SXT) onboard AstroSat, when the source was in the hard spectral state as revealed by the broad-band spectra. The spectra obtained from all the observations can be described by a single-temperature Comptonizing region with disc and reflection components. The event mode data from LAXPC provides unprecedented energy dependent fractional root mean square (rms) and time-lag at different frequencies which we fit with empirical functions. We invoke a fluctuation propagation model for a simple geometry of a truncated disc with a hot inner region. Unlike other propagation models, the hard X-ray emission (&gt;4 keV) is assumed to be from the hot inner disc by a single-temperature thermal Comptonization process. The fluctuations first cause a variation in the temperature of the truncated disc and then the temperature of the inner disc after a frequency dependent time delay. We find that the model can explain the energy dependent rms and time-lag at different frequencies.
Impact of anthocyanin-rich whole fruit consumption on exercise-induced oxidative stress and inflammation: a systematic review and meta-analysis
Authors: Bloedon Taylor K , Braithwaite Rock E , Carson Imogene A , Klimis-Zacas Dorothy , Lehnhard Robert A 
Published: March 2019

Abstract

Context
Supplementing with fruits high in anthocyanins to reduce exercise-induced oxidative stress and inflammation has produced mixed results.

Objective
This systematic review and meta-analysis aims to discuss the impact of whole fruits high in anthocyanins, including processing methods and the type and amount of fruit, on inflammation and oxidative stress.

Data Sources
PICOS reporting guidelines and a customized coding scheme were used to search 5 databases (SPORTDiscus, Science Direct, Web of Science [BIOSIS], Medline [Pubmed], and the Cochrane Collaboration) with additional cross-referencing selection.

Data Extraction
A random-effects meta-analysis was used to measure effects of the fruit supplements with 3 statistics; the QTotal value based on a χ2 distribution, τ2 value, and I2 value were used to determine homogeneity of variances on 22 studies (out of 807). Outliers were identified using a relative residual value.

Results
A small significant negative summary effect across the sum of all inflammatory marker outcomes (P < 0.001) and a moderate negative effect for the sum of all oxidative stress marker outcomes (P = 0.036) were found. Moderator analyses did not reveal significant (P > 0.05) differences between subgrouping variables.

Conclusions
Results indicate that consumption of whole fruit high in anthocyanins can be beneficial for reducing inflammation and oxidative stress.
Screening practices for paediatric asymptomatic adrenal suppression in Canada: Are we addressing this important risk?
Authors: Goldbloom Ellen B , Ahmet Alexandra 
Published: March 2019

AbstractBackgroundChildren with adrenal suppression (AS), a potential side effect of glucocorticoids (GCs) may be asymptomatic, present with nonspecific signs and symptoms or with adrenal crisis. Asymptomatic AS (AAS) can only be diagnosed through screening. Identifying and treating asymptomatic patients before symptoms develop may reduce morbidity. Screening guidelines for AS are lacking. Consequently, screening practices are highly variable.ObjectiveTo assess (1) the screening practices for and recognition of paediatric AAS among clinicians in Canada and (2) the educational impact of a 2-year surveillance program of symptomatic AS cases.MethodsBefore and after a 2-year Canadian Paediatric Surveillance Program (CPSP) study of symptomatic AS, participants were surveyed through the CPSP. The prestudy survey was sent to 2,548 participants in March 2010 and the poststudy survey was sent to 2,465 participants in April 2013.ResultsResponse rates were 32% for the prestudy survey and 21% for the poststudy survey. Between the pre- and poststudy surveys, the percentage of physicians who reported routinely screening patients on GCs for AS increased from 10% to 21% and the percentage who reported having a screening policy in their office/centre increased from 6% to 11%. There was no significant change in the percentage of physicians who had diagnosed a child/youth with AAS in the preceding year.ConclusionFrequency of screening for AAS increased following the 2-year study but remains low. Development of a clinical practice guideline should increase both awareness of asymptomatic AS among Canadian paediatricians and the identification of AAS, before symptoms develop.
Exercise-Based Rehabilitation to Improve Exercise Capacity and Quality of Life in Pulmonary Arterial Hypertension
Authors: Babu Abraham S , Holland Anne E , Morris Norman R 
Published: March 2019

Abstract
&lt;LEAP&gt; highlights the findings and application of Cochrane reviews and other evidence pertinent to the practice of physical therapy. The Cochrane Library is a respected source of reliable evidence related to health care. Cochrane systematic reviews explore the evidence for and against the effectiveness and appropriateness of interventions—medications, surgery, education, nutrition, exercise—and the evidence for and against the use of diagnostic tests for specific conditions. Cochrane reviews are designed to facilitate the decisions of clinicians, patients, and others in health care by providing a careful review and interpretation of research studies published in the scientific literature. Each article in this PTJ series summarizes a Cochrane review or other scientific evidence on a single topic and presents clinical scenarios based on real patients or programs to illustrate how the results of the review can be used to directly inform clinical decisions. This article focuses on people diagnosed with pulmonary arterial hypertension. Can exercise-based rehabilitation programs improve exercise capacity and quality of life in people with pulmonary arterial hypertension?
TDP-43 proteinopathy impairs neuronal mRNP granule mediated postsynaptic local translation and mRNA metabolism
Authors: Wong Chia-En , Tsai Kuen-Jer 
Published: March 2019

AbstractLocal protein synthesis and mRNA metabolism mediated by mRNP granules in the dendrites and the postsynaptic compartments is essential for synaptic remodelling and plasticity in the neuronal cells. Misregulation in these processes caused by TDP-43 proteinopathy lead to neurodegenerative diseases such frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). Using biochemical analysis and imaging techniques including super-resolution microscopy, we provide evidences for the first time of the postsynaptic localization of TDP-43 in the mammalian synapses; and we show TDP-43 as a component of neuronal mRNP granules. With activity stimulation and different molecular approaches, we further demonstrate activity-dependent mRNP granule dynamics involving disassembly of mRNP granules, release of mRNAs, and activation of local protein translation as long as impairments in models of TDP-43 proteinopathy. This study elucidates the interplay between TDP-43 and neuronal mRNP granules in normal physiology and TDP-43 proteinopathy in regulation of local protein translation and mRNA metabolism in the postsynaptic compartment.
CD2 expression acts as a quantitative checkpoint for immunological synapse structure and T-cell activation
Authors: Demetriou Philippos , Abu-Shah Enas , McCuaig Sarah , Mayya Viveka , Valvo Salvatore , Korobchevskaya Kseniya , Friedrich Matthias , Mann Elizabeth , Lee Lennard YW , Starkey Thomas , Kutuzov Mikhail A. , Afrose Jehan , Siokis Anastasios ,   Oxford IBD Cohort Investigators, Meyer-Hermann Michael , Depoil David , Dustin Michael L. 
Published: March 2019

AbstractThe CD2 receptor has been described as an adhesion and costimulatory receptor on T cells. Here, transcriptional profiling of colorectal cancers (CRC) revealed a negative correlation betweenCD2expression and “exhausted CD8+T-cells” gene signatures. Furthermore, we detected reduced surface CD2 levels in exhausted CD127lowPD-1hiCD3+CD8+tumour infiltrating lymphocytes (TILs) in CRC. We describe a CD2 expression-level-dependent switch in CD2-CD58 localization between central and peripheral domains in the immunological synapse (IS). A peripheral “CD2 corolla” formed when CD2 surface expression was sufficiently high and its cytoplasmic domain intact. The corolla recruited other ligated receptors like CD28, boosted recruitment of activated Src-family kinases (pSrc), LAT and PLC-γ in the IS and consequently T-cell activation in response to a tumour antigen. Corolla formation and pSrc in the IS increased linearly with CD2 expression, whereas pSrc signals were reduced by high, “exhausted-like” levels of PD-1, which invaded the corolla. These results suggest two levels of inhibition of Src-family kinases in CD3+CD8+TILs: reduced CD2 expression and high PD-1 expression.
Antagonistic coevolution between hosts and sexually transmitted infections
Authors: Ashby Ben 
Published: March 2019

AbstractSexually transmitted infections (STIs) are predicted to play an important role in the evolution of host mating strategies, and vice versa, yet our understanding of host-STI coevolution is limited. Here, I present a model of acute STI infection in populations with ephemeral mating dynamics, where hosts evolve their preference for healthy mates and STIs evolve mortality or sterility virulence. Mate choice readily evolves even though ephemeral mating and acute infections reduce the advantages of mate choice compared to previous theory based on serial monogamy and chronic infections. Selection for mate choice constrains both mortality and sterility virulence, leading to optimal strategies in each population, host polymorphism, or fluctuating selection. I show how the mode of virulence, costs associated with mate choice, recovery, and host lifespan impact on host-STI coevolution, with fluctuating selection most likely when hosts have intermediate lifespans, STIs cause sterility and longer infections, and costs of mate choice are not too high. The results reveal new insights into the evolution of mate choice and how coevolution unfolds for different host and STI life-history traits, providing increased support for parasite-mediated sexual selection as a potential driver of host mate choice, and mate choice as a constraint on STI virulence.