bluuebunny/crossref_metadata_2025_split huggingface dataset:
The resistant ability to weevils and the characteristics of VrPDF1 gene of some mungbean cultivars (Vigna radiata L.Wilczek)
Authors: Thao Hoàng Thị , Tường Hồ Mạnh , Lan Nguyễn Thị Ngọc , Thanh Nguyễn Vũ Thanh , Sơn Nguyễn Văn , Mậu Chu Hoàng 
Published: April 2018

Plant defensins play a role against the seed-feeding insects. Defensin associates with the center of α-amylase activity in the gut of weevils, thus inhibiting the digestion of starch by weevils. In this study, the resistance of eight mungbean cultivars to weevils was evaluated by the method of artificial weevils infection. The Tam TH cultivar had lowest index of susceptibility to weevils (634.63) and DX22 cultivar had highest index (1058.72), and the highest resistance to weevils was found in Tam TH and DX22 was found to have the lowest resistance. VrPDF1 genes isolated from mungbean cultivars are 356 bp in length with two exons interrupted by an intron. The coding region of the VrPDF1 gene is 228 bp in length, encoding 75 amino acids. The comparative results of the nucleotide sequence of cDNA between Tam TH and DX22 showed that there was a difference in 13 nucleotides and comparison of amino acid sequences of the deduced protein indicated that there was a difference in 9 amino acids. Within the intron region of the VrPDF1 genes there was difference in 5 nucleotides. The genetic distance based on nucleotide sequences of the coding region of VrPDF1 gene of DX22 and seven other mungbean cultivars is 6.2% and based on the amino acid sequence deduced is 7.7%. The coding region of the VrPDF1 gene of DX22 was used to create a transformation vector aimed at creating weevil-resistant transgenic mungbean.
Research on in vitro micropropagation of Lilium brownii F.E. Brown
Authors: Sâm Vũ Hoài , Quỳnh Bùi Đức , Hương Nguyễn Thị , Khiêm Nguyễn Văn 
Published: April 2018

Lilium brownii Brown belonging Lilium genus and Liliaceae family is well-known as a popular medicinal species, as well as food source and beautiful ornamental flowers. The specie has unique and ornamental floral characteristics such as light and elegant fragrance and perianth color rapidly changing from yellowish cream to white during anthesis. In traditional medicine, it is used for treatment cough, sedation diuretic, bronchitis... In nature, it can be found in subtropical climate moutainous areas in the North such as Sa Pa, Bat Xat, Mu Cang Chai; Sin Ho and Phong Tho, Quang Ba and Dong Van. In recent years, this species has been listed in the Red List for medicinal plants in Vietnam due to over-exploitation. The only effective strategy for sustaible conservation this species is in vitro micropropagation. In this study, in vitro plant regeneration and micropropagation of L. brownii was established from bubles and stem nodes. After surface sterilization with 0.1% HgCl­2 in 10 minutes, healthy young shoots were obtained from initial bubles and stem nodes on MS medium supplemented with 0.5 mg/l BAP or 0.5 mg/l NAA, respectively. Bulblets also were formed from young shoot on MS supplemented with 0.5 mg/l NAA. The highest number of 4.5 bulblets per an explant was recorded from longitude-divided bubbles on MS medium containing 0.5 mg/l NAA and 0.2 mg/l BAP after 60 days in culture. The regererated plants produced quality roots on half strength MS supplemented with the combination of 1.0 mg/ l NAA and 0.2 mg / l BAP. More than 90% of rooted plants in vitro were survival on artificial soil TN1 in the nursery.
Characteristics of defensin1 gene and designing structure to create resistant transgenic corn lines to weevils
Authors: Thủy Vì Thị Xuân , Thu Lò Thị Mai , Tường Hồ Mạnh , Sơn Lê Văn , Thanh Nguyễn Vũ Thanh , Mậu Chu Hoàng 
Published: April 2018

Plant defensins are multifunctional proteins, inhibiting the growth of fungal, anti-bacterial, altering membrane channels, inhibiting activity of trypsin and α-amylase. Plant defensin consists of 18 groups in which the group 1 includes defensins to inhibit either α-amylase enzyme or trypsin. Defensins bind to the active site of α-amylase in the weevil gut, thus inhibit starch digestion in weevils. In this report, we present the results of cloning and determining the ZmDEF1 gene sequence isolated from mRNA and DNA of Sonla province local maize and LVN99 hybrid maize cultivar. The coding region of ZmDEF1 gene isolated from some maize samples had the size of 243 nucleotides, encoding 80 amino acids. Gen ZmDEF1 isolated from DNA had the size 345bp consists of two exons and one in tron (102 bp). The nucleotide sequences of ZmDEF1 gene (DNA) of the samples have 6 positions nucleotide difference, on exon 1 has two points difference (position 43, 53), on intron has a difference (position 150), on exon 2 has 3 nucleotide site difference (203, 263 and 297 position). Deduced amino acid sequences of defensin of the Sonla local maize sample has 8 cysteines to make 4 disulfide bridges, while LVN99 hybrid maize has 7 cysteines, which can formed only 3 disulfide bridges. Transformation vector pBetaPhaso-ZmDEF1 has been designed successfully, in which ZmDEF1 is controlled by seed specific Phasoline promoter. The correct insertion and expression of ZmDEF1 was examinated in transgenic tobacco plants throught PCR and RT-PCR, respectively. These results provide an firm evident for using the designed transformation vector to produce transgenic maỉze lines with an improved resistant ability to weevils.
Optimization of β-D-galactosidase rapid enzyme assay using Escherichia coli ATCC 8739
Authors: Tu Le Thi Anh , Le Le Ba 
Published: April 2018

The bacterial enzyme, β-D-galactosidase, catalyzes the breakdown of the complex sugar lactose into its components - galactose and glucose- simple sugars. The glycolysis of lactose by β-D-galactosidase is a point of attack for studies of the biochemical problem in disaccharide utilization and the genetic basis of enzyme constitution β-D galactosidase. The aim of the present study was to focus on optimization of a rapid enumeration method based on the enzymatic hydrolysis of 4-methylumbelliferyl-β-D-galactoside (MUGal) for toxicity test using Escherichia coli (E. coli) ATCC 8739 as a model. This rapid assay is based on the assumption that β-D-galactosidase is one marker for E. coli ATCC 8739. The enzymatic activity of E. coli ATCC 8739 was measured in a 25-minute assay. The effects of pH, temperature, nutrition, and substrate concentrations on enzyme activity were investigated. The enzyme β-D-galactosidase was shown to be induced with the inducer isopropyl-beta-D-thiogalactopyranoside (IPTG). The high level of production of β-D-galactosidase was found at bacteria incubated in tryptic soy broth without dextrose with IPTG. The optimum pH and temperature for β-D-galactosidase activity of E. coli ATCC 8739 was found to be 6.8 and 44.5 oC, respectively. The enzyme activity detected increased when raising the concentrations of the substrate (MUGal). The good linear correlation between logarithms of enzyme activities and CFU showed following supported the use of this method for toxicity experiments. Enzymatic methods and reference plate counts were significantly correlated. This method is sensitive, simple to perform and can be used as an alternative for traditional methods in detecting cell viability.
Lipid biosynthesis of ten thraustochytrid strains isolated from mangrove Xuan Thuy, Nam Dinh
Authors: Đào Phạm Thị Bích , Tuấn Nguyễn Đình , Khoa Trần Đăng , Huyên Chử Thị , Thành Đỗ Hoàng , Hà Nguyễn Thị Hoài 
Published: April 2018

The features of polyunsaturated fatty acid-PUFA structures were corresponded to each separate functions as adjusting the cellular physiology and gene expression. Therefore, lack of PUFA could lead to abnormalities in skin, kidney, neural networks, immune responses and inflammation; cardiovascular, endocrine, respiratory and reproductive systems. In fish oil, PUFA content were low, thus it was difficult to produce on a large scale. Therefore, the exploration of PUFA sources particularly as arachidonic acid-AA, eicosapentaenoic acid EPA, docosapentaenoic acid-DPA/DHA attracted many researches. Heterotrophic microalgae Thraustochytrids were capable of producing high amounts of DHA and PUFA composition varied. DHA can be synthesized by the metabolism of AA, EPA and DPA. The different types of PUFA reflected relationships in classification. Ten heterotrophic microalgae thraustochytrids isolated from mangrove Xuan Thuy, Nam Dinh contain fatty acid composition varied from C12 to C28. Especially, they had two important fatty acids of PUFA as EPA and DPA. Polyunsaturated fatty acids - PUFA content of ten thraustochytrid strains were from 28.95 to 49.62% total lipid. DPA compared to other PUFA were high for all thraustochytrid strains studied, accounting 20.22 to 39.35% TFA. Ten thraustochytrid strains had the highest growth with carbon source as glucose, total lipid reached 7 to 12.35 % dry weight biomass after 72 hours. Growth rate and lipid biosynthesis in organic nitrogen source were higher than in inorganic nitrogen sources. The best source of nitrogen for growth and lipid biosynthesis of ten thraustochytrid strains is yeast extract, total lipid were 8.57 to 18.87% dry weight biomass after 72 hours.
Qualification and quantification of huperzine a from Huperia serrata in Da Lat, Lam Dong province
Authors: Ngọc Vũ Thị , Hạnh Phạm Thị , Anh Lê Thị Lan , Đạt Nguyễn Tiến , Thủy Lê Thị Bích 
Published: April 2018

Huperzine A, an alkaloid, was originally isolated from Huperzia serrata. This compound potentially enhances the memory in animal, hence, it has been approved as a drug for the clinical treatment of Alzheimer’s disease, a major disease affecting the elderly population throughout the world. Because Huperzine A is an acetylcholinesterase inhibitor, the presentation of Huperzine A in brain inhibited acetylcholinesterase activity, thus, leading to the increase in concentration of acetylcoline. In Vietnam, H. serrata distributed in Sapa (Lao Cai) and Da Lat (Lam Dong), this species provide valuable pharmaceutical materials to the treatment for Alzheimer’s diseases. In this research, we evaluated the availability of Huperzine A in Huperzia serrata, which was collected from Da Lat (Lam Dong) in two seasons: Spring and Autunm. Thin layer chromatography (TLC) method was used to preliminary qualitative analysis. High performance liquid chromatography (HPLC) method were used for determining Huperzine A content in samples. In the result, Huperzine A is almost existed in leaves of Da Lat Huperzia serrata and equivalent levels of Chinese Huperzia serrata. The Content of Huperzine A was different between two collection samples in Spring and Autumn, by analyze HPLC data, the samples was harvested in Autumn contents 92.5 µg.g-1dry sample and the spring is 75.4 µg.g-1 dry sample. Therefore, the content of Huperzine A in Huperzia serrata’s leaves sample is harvested in the fall compared with samples collected in the spring is higher 17.1 µg.g-1dry samples.
Investigation of explants sterilization process, effect of light intensity and concentration of agar for callus induction of Kappaphycus alvarezii (Doty) doty (Rhodophyta) in vitro
Authors: Mơ Vũ Thị , Reddy CRK 
Published: April 2018

Objective of this study was to ascertain the optimum condition for callus induction of K. alvarezii (Doty) in vitro such as to determine the explants sterilization process, the effect of intensity of light and the concentration of agar. Fresh thalli treated with 0.5% - 1% detergent for 5 mins followed by 0.5% - 1% betadine for 2 – 3 mins and incubated with 0.5% - 1% broad spectrum antibiotic mixture in PES medium for 1 day produced 95 – 98% bacteria free healthy explants. Two independent experiments with light intensity and agar contentration of the environment were carried out at 5 different levels of 0, 5, 25, 50, 70 µmol photon.m2.s-1 and 9 agar concentrations of 0.5%, 0.75%; 1.0%, 1.25%, 1.5%, 1.75%, 2.0%, 2.5%, 3.0%. The highest callus induction rate was (96 ± 3.5 – 98 ± 2.1%) at 5 - 25 µmol photon.m-2.s-1 and (87 ± 5.8% – 90 ± 5.0%) in 1% - 3% agar concentration after 2 weeks of explants. The highest callus living rate was 98% at the light intensity of 25 µmol photon.m-2.s-1 and (75 ± 5.7 – 84 ± 1.1%) in 0.75 – 1.5% agar concentration after 2 months of explants. The highest callus re-induction rate was 50 – 55% at the light intensity of 5 – 25 µmol photon.m-2.s-1 and 60 – 65% in 1 – 1.5% agar concentration. Callus was not observed in dark condition (0 µmol photon.m-2.s-1). These calluses, that were strong, big and had filamentous type, will be a good material for the next production stage of embryonic callus production and seedling regeneration from micropropagules.
Isolation of insect parasitic fungi Corrdyceps spp. rich in bioactive beauvericin from Pu Mat National Park, Nghe An
Authors: Lan Nguyễn Thị Thanh , Thúy Đinh Thị Ngọc , Bình Nguyễn Thị Thanh 
Published: April 2018

Insect parasitic fungi Cordyceps sp. B6 was isolated as single spores from fungi on the host insect pupae of Lepidoptera, collected at Pu Mat National Park, Nghe An, Vietnam. Cordyceps B6 strain was identified by the method of molecular biology, in which the DNA of the strain was extracted, cleaned and used as a template to amplify the gene segment using primers for ITS1 and ITS2. ITS gene segment of the strain was sequenced and compared to sequences in the GeneBank database using BLAST (NCBI). With the similarity of 99% compared to the corresponding gene fragment of Cordyceps takaomontana, Cordyceps B6 strain was identified as C. takaomontana. C. takaomontana B6 was best activated with liquid medium CT1 containing glucose 20 g/l, yeast extract 5 g / l, peptone 10 g/l, KH2PO4 1 g/l, 0.5 g MgSO4/l . In this medium, the density could reach 5 x 106 spores cells/ml after 7 days of culture. When surface fermentation performed in appropriate conditions (25 °C, pH= 7, light intensity 200 lux, humidity 80%), the fresh weight of 18.72 g/vial of spore cells and 2.09 mg of active beauvericin per gram of freeze-dry weight were obtained after 45 days of culture. With a high concentration of active beauvericin, C. takaomontana B6 could be considered as a potential source for development of functional food and raw materials for the pharmaceutical industry.
Yukarı Kızılırmak Havzası’nda ırmak sularının tarımda sulama amaçlı kullanım özelliklerinin belirlenmesi üzerine bir araştırma
Authors: Koç Hakan , Doğru Derya , Han Emre 
Published: April 2018

Bu araştırmanın amacı; Yukarı Kızılırmak Havza sınırları içerisinde, Kızılırmak’ın, tarımsal sulama amaçlı kullanım özelliklerini Wilcox ve ABD tuzluluk diyagramlarına göre belirlemek ve bu havza sınırları içerisindeki Kızılırmak’ın kimyasal özelliklerine etki eden coğrafi faktörleri tespit etmektir. Araştırma sahasından veriler birincil kaynaklardan (su numunesi, toprak numunesi ve kayaç numunesi) ve ikincil kaynaklardan (meteorolojik veriler, Devlet Su İşleri 19. Bölge Sivas Müdürlüğünden alınan su analiz raporları vb.) temin edilmiştir. Su numunelerin tarımda sulama amaçlı kullanılıp kullanılamayacağını belirlemek için Wilcox ve ABD tuzluluk diyagramlarından yararlanılmıştır. Toprak analiz sonuçları betimsel analiz yöntemine göre değerlendirilmiştir. Sivas Meteoroloji İl Müdürlüğünden alınan meteorolojik veriler, Thornthwaite iklim sınıflaması yöntemine göre değerlendirilmiş ve su bilançoları hesaplanmıştır. Araştırma sonucunda aşağıdaki bulgu ve sonuçlara ulaşılmıştır: 1. Alınan su numunelerinden Yukarı Kızılırmak Havza sınırlarındaki ırmak suyunun, Wilcox ve ABD tuzluluk diyagramlarına göre değerlendirmeleri sonucunda, Kızılırmak suları kaynağında yumuşak ve içilebilir durumda iken Zara ilçesinde başlayarak özellikle de Hafik ilçesinden sonra Kayseri il sınırına kadar sulama suyu olarak kullanılması şüpheli uygun değil veya kullanılmaması uygun olmayan sular sınıfına girmektedir. 2. Araştırma sahasından alınan kayaç numuneleri değerlendirildiğinde; Zara'dan Kayseri il sınırına kadar ise havzanın büyük bir kısmının jips serilerinden oluşması suların çok yüksek tuzlu ve acı olmasına neden olmuştur. Bu durum Kızılırmak sularının tarımda sulama amaçlı kullanımını olumsuz yönde etkilemiştir. 3. Kızılırmak ve onu besleyen kollara yağış yaz mevsimlerinde yeterince yağış girmediğinden dolayı Kızılırmak sularındaki tuzlar seyrelememekte ve bölgede yaşanan kuraklık Kızılırmak'ta ki tuz miktarını yaz aylarında artırmaktadır. Bu durum Kızılırmak suyunun yaz aylarında tarımsal sulama amaçlı yeterinde yararlanılamamasına sebep olmaktadır.
1949 Sonrası Hariçte Doğu Türkistan Davası ve Tayvan Yönetiminin Tutumu
Authors: KUL Ömer 
Published: April 2018

1949 yılında Doğu Türkistan’ın Komünist Çin işgaline
uğramasıyla vatanlarından ayrılan ve büyük çoğunluğu Türkiye’ye yerleşen Doğu
Türkistanlılar, davalarını dünya kamuoyuna duyurabilmek için büyük bir çaba ve
yoğun bir çalışma içerisine girmiştir. Doğu Türkistanlıların, Komünist Çin'e
karşı azmi, gayreti ve heyecanını kendi siyasi beklentileri doğrultusunda
kullanmak isteyen Tayvan yönetimi, aynı zamanda Doğu Türkistanlıların birlik,
beraberlik ve kararlılığını ortadan kaldırmak için sinsi bir politika uygulamıştır.
Tayvan yönetiminin sinsi siyasetine alet olan bir kısım Doğu Türkistanlılar,
davalarına zarar vermekle kalmamış, daha kötüsü birbirlerine düşmüştür.
Önceleri sözlü olarak yapılan karalama kampanyaları daha sonra yazılı basın
yoluyla iftiralara kadar varmıştır.

Bu çalışmada, Tayvan yönetiminin bir kısım Doğu
Türkistanlıları kendi politik çıkarları için nasıl suiistimal ettiği ve zaman
içerisinde bir kısım Doğu Türkistanlıların nasıl birbirlerine düştükleri ortaya
konulmaya çalışılmıştır.&nbsp;
Geleneksel Ekolojik Bilgi Bağlamında Çanakkale Halk Botaniği
Authors: YOLCU Mehmet Ali 
Published: April 2018

Doğa bilgisi
genellikle karmaşık bir ampirizmin ürünü olmakla birlikte bu deneyimden
beslenen bilgi, kültür ve doğa karşıtlığında ortaya çıkan sınırların muğlaklığı
noktasında geleneksel toplumların çevresel uyum becerilerini bize
göstermektedir. Bu bağlamda geleneksel ekolojik bilgi; kültürel aktarım
araçlarıyla kuşaktan kuşağa iletilen, ekolojinin bir parçası olan nesnelerle
ilgili insanın bilgi ve inanç birikiminin bir yansıması olarak karşımıza
çıkmaktadır. İnsan ve çevre ilişkisine dayalı ortaya çıkan geleneksel ekolojik
bilginin bir alt kolu olan halk botaniği, bitkilerin adlandırılmalarından
kullanım biçimlerine, bitkilerle ilgili inançlardan büyüsel uygulamalara kadar
bir dizi konuyu kapsamaktadır. Çanakkale yöresinde Güney Marmara ikliminin
etkisiyle yetişen çeşitli ot, ağaç ve mantar türlerinin insanların günlük
hayatında önemli işlevleri bulunmaktadır. Yörenin bitki örtüsü hem Kaz Dağları
hem de Marmara ve Ege denizi etkisi nedeniyle zengindir. Makalede geleneksel
ekolojik bilgi çerçevesinde Çanakkale yöresinde halk botaniği unsurları
incelenmiştir.
Implementação de Práticas de Produção Enxuta: Estudo de Caso de uma Fábrica de Redutor de Velocidade
Authors: Bogado Bruna Natália da Silva , Placca José Avelino 
Published: April 2018

Este trabalho tem como objetivo analisar a implementação de práticas da produção enxuta em um fábrica de redutores de velocidade. Serão analisados aspectos desde a compra da matéria prima, distribuição de peças nas máquinas e meios de processo. A mudança de paradigmas vem ocorrendo por meio de pequenas etapas uma vez que a maneira de se chegar ao objetivo começa nas pequenas ações, atitudes e cultura que os trabalhadores têm e que, muitas vezes, tem que ser mudada em vários aspectos para que o processo seja efetivo. O presente trabalho relata a busca da causa raiz de diversas dificuldades outrora enfrentadas pela empresa e a implantação das ações definidas pela aplicação dos conceitos de Produção Enxuta. O presente trabalho permeou as áreas PPCP, Engenharia Industrial, Tratamento Térmico Almoxarifado e Suprimentos da empresa. Muitas ferramentas da Produção enxuta serão apresentadas neste estudo dando-se ênfase aos resultados obtidos. O sistema Lean Production gerou muitos ganhos, tanto qualitativos como quantitativos que serão apresentados no decorrer do trabalho. Em contra-partida serão apresentadas as dificuldades e resistências que foram enfrentadas na sua implantação e principalmente na manutenção das mudanças efetuadas, sendo provavelmente este o processo mais difícil. Serão abordadas possíveis soluções todas elas alicerçadas em muita determinação, foco, atitude e disciplina. Este trabalho também nos mostrou a importância de considerarmos os aspectos humanos na implementação das diversas práticas do Lean Production. O comprometimento e trabalho em equipe mostrou-se essencial para ocorrer a efetiva mudança.
Oscar Wilde’s Aestheticism
Authors: Guan Beibei 
Published: April 2018

<p> <em>Abstract</em>-Dandyism is a very important and significant social phenomenon in 19<sup>th</sup> century Europe. This paper focuses on Oscar Wilde and Wilde’s numerous works. Aestheticism was used as a tool by the dandy in his rebellious performances in London, manifesting the contradiction between the spiritual and the material, the aristocracy and the bourgeoisie, and art and nature. The social backgrounds and life experiences of Wilde influenced his transformation into dandies during the time of the Victorian period. With his strong sense of fashion and style, and their elegant ironic use of language, the dandies focused on the importance of artificial forms - himself included — in daily life and work. The dandies’ concern was pleasure seeking through consuming the visual and actual. To this end Wilde developed unique aesthetic theories regarding evil; he looked for the beautiful in the ugly and repulsive. At the same time, Oscar Wilde criticized the hypocrisy of bourgeois morality and values, showing the genuinely ugly and evil reality beneath bourgeois industrial society, and focusing on revolt and resistance against the bourgeois world.</p><p><em>Index Terms</em>-Dandyism, Aestheticism, Revolt</p>
Gender Nonconformity and Casting around Individuality, Free Will and Survival: Sharat Chandra’s Women in His Novel Srikanta
Authors: Jahan Sultana 
Published: April 2018

<p>The present paper is a sincere effort to explore the image of Indian women in the early 19th century social context as depicted in Sharat Chandra’s novel S<em>rikanta</em>. In this novel Sharat Chandra’s portrayal of women characters- Rajlaksmii, Annada, Abhaya , and Kamal Lata assert their individuality, self-worth and deliverances boldly in the then male-controlled and traditional society. These characters are unwavering and resolute enough to cast around an emancipated futuristic outlook. They are all precursors to the later day women characters depicted by the feminist writers. Sharat chandra is not a feminist in the traditional sense nor does he take the side of forceful assertion of women rights but he shows a significant understanding of woman psyche and to a great extent, protests against social and religious double standard that ultimately results in gender nonconformity. He values humanity more than chastity and raises his voice against traditional morality and religious dogmatism in depicting illicit love relationship and in disclosing the deceptions underlying the established marriage custom. To all female characters, Rajlaksmi, Annada,Kamal Lata, and Abhay, marriage fails to provide congenial atmosphere to love and value each other; rather to them, marriage is nothing but religious and social yolk that come up with patriarchal applaud but result in self-deception. This paper is an attempt to elucidate Sharat Chandra’s unconventional idea of chastity and reversed roles of women going deep into the female characters of this novel who fearlessly look down on the patriarchal impediments.</p>