bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA Methylation of Mouse Testes, Cardiac and Lung Tissue During Long-Term Microgravity Simulation
Authors: Loktev Sergey S. , Ogneva Irina V. 
Published: May 2019

AbstractUnder microgravity, the gene expression levels vary in different types of cells; however, the reasons for this have not been sufficiently studied. The aim of this work was to evaluate the methylation of CpG islands in the promoter regions of the genes encoding some cytoskeletal proteins, the total methylation and 5 hmC levels, and the levels of enzymes that regulate these processes in the testes, heart, and lungs in mice after a 30-day microgravity modeling by antiorthostatic suspension and after a subsequent 12-hour recovery as well as in the corresponding control group and identical groups treated with essential phospholipids. The obtained results indicate that under modeling microgravity in the examined tissues a decrease of cytoskeletal gene expression (mainly in the heart and lungs tissues) correlated with an increase in the CpG islands methylation and an increase of the expression (mainly in the testes tissue) – with a decrease of the CpG-methylation, despite of the fact that in the examined tissues took place a decrease of the content methylases and demethylases. But the deacetylase HDAC1 content increased in the heart and lungs tissues and decreased in the testes, letting us suggest its participation in the regulation of the methylation level under microgravity conditions.
Alteration of erythrocyte membrane polyunsaturated fatty acids in preterm newborns with retinopathy of prematurity
Authors: Pallot Charlotte , Mazzocco Julie , Meillon Cyril , Semama Denis S. , Chantegret Corinne , Ternoy Ninon , Martin Delphine , Donier Aurélie , Gregoire Stéphane , Creuzot-Garcher Catherine P. , Bron Alain M. , Bretillon Lionel , Acar Niyazi 
Published: May 2019

AbstractExtremely preterm infants are at high risk for retinopathy of prematurity (ROP), a potentially blinding disease characterized by abnormalities in retinal vascularization. Whereas animal studies revealed that n-3 polyunsaturated fatty acids (PUFAs) may be of benefit in preventing ROP, human studies conducted on preterm infants during the 1st weeks of life showed no association between blood n-3 PUFA bioavailability and ROP incidence and/or severity, probably because of the influence of nutrition on the lipid status of infants. In the OmegaROP prospective cohort study, we characterized the erythrocyte concentrations of PUFAs in preterm infants aged less than 29 weeks gestational age (GA) without any nutritional influence. We show that GA is positively associated with the erythrocyte n-6 to n-3 PUFA ratio, and particularly with the ratio of arachidonic acid (AA) to docosahexaenoic acid (DHA), in infants with ROP. A time-dependent accumulation of AA at the expense of DHA seems to occur in utero in erythrocytes of preterm infants who will develop ROP, thus reinforcing previous data on the beneficial properties of DHA on this disease. In addition, preliminary data on maternal erythrocyte membrane lipid concentrations suggest modifications in placental transfer of fatty acids. Documenting the erythrocyte AA to DHA ratio at birth in larger cohorts might be useful to set up new prognostic factors for ROP.
Enhanced osteogenic proliferation and differentiation of human adipose-derived stem cells on a porous n-HA/PGS-M composite scaffold
Authors: Wang Yaozong , Sun Naikun , Zhang Yinlong , Zhao Bin , Zhang Zheyi , Zhou Xu , Zhou Yuanyuan , Liu Hongyi , Zhang Ying , Liu Jianguo 
Published: May 2019

AbstractThis study explored the applicability, cellular efficacy, and osteogenic activities of porous nano-hydroxyapatite/Poly (glycerol sebacate)-grafted maleic anhydride (n-HA/PGS-g-M) composite scaffolds. Nuclear magnetic resonance (NMR) analyses indicated that approximately 43% of the hydroxide radicals in PGS were displaced by maleic anhydride. Resonance bands at 1036 cm−1 occurred in scaffolds containing nHA powders, and peak areas increased when n-HA weight increased in PGS-M-n-HA-0.4, PGS-M-n-HA-0.5, and PGS-M-n-HA-0.6 scaffolds. The n-HA/PGS-g-M composite scaffolds exhibited porous microstructure with average pore size of 150–300 µm in scanning electron microscopy (SEM) analysis. Differential scanning calorimetry (DSC) identified the glass transition temperature (Tg) as −25–30 °C, indicative of quality resilience. The modulus of compressibility increased when n-HA content increased. Interestingly, viability of human adipose-derived stem cells (hADSCs) in vitro and expression of the osteogenic related genes RUNX2, OCN, and COL1A1 was enhanced in the n-HA/PGS-g-M composite scaffolds compared to those factors observed in PGS-g-M scaffolds. Finally, simulated body fluid (SBF) tests indicated more apatite deposits on the surface of n-HA/PGS-g-M scaffolds compared to PGS-g-M scaffolds. Overall, porous n-HA/PGS-g-M composite scaffolds possessed acceptable biocompatibility and mechanical properties, and they stimulated hADSC cell proliferation and differentiation. Given these qualities, the composite scaffolds have potential applications in bone tissue engineering.
Role of deleterious single nucleotide variants in the coding regions of TNFAIP3 for Japanese autoimmune hepatitis with cirrhosis
Authors: Higuchi Takashi , Oka Shomi , Furukawa Hiroshi , Nakamura Minoru , Komori Atsumasa , Abiru Seigo , Hashimoto Satoru , Shimada Masaaki , Yoshizawa Kaname , Kouno Hiroshi , Naganuma Atsushi , Ario Keisuke , Kaneyoshi Toshihiko , Yamashita Haruhiro , Takahashi Hironao , Makita Fujio , Yatsuhashi Hiroshi , Ohira Hiromasa , Migita Kiyoshi 
Published: May 2019

AbstractAutoimmune hepatitis (AIH) is an autoimmune liver disease and cirrhosis is sometimes complicated with AIH at diagnosis, influencing its prognosis.TNFAIP3gene encodes A20, an inhibitor of nuclear factor-κB pathway, and is a susceptibility gene for autoimmune diseases. We investigated deleterious variants in the coding regions ofTNFAIP3gene of Japanese AIH patients or those with cirrhosis. The deleterious variants in the coding regions ofTNFAIP3gene were analyzed by the cycle sequencing method and the frequencies of deleteriousTNFAIP3alleles of AIH or AIH with cirrhosis were compared with those of Japanese controls. The deleterious alleles inTNFAIP3were not associated with AIH. A significant association was shown for the deleterious alleles inTNFAIP3(P = 0.0180, odds ratio (OR) 4.28, 95% confidence interval (CI) 1.53–11.95) with AIH with cirrhosis at presentation. The serum IgM levels in AIH patients with deleterious alleles inTNFAIP3were tended to be lower than those without (P = 0.0152,Q = 0.1216). The frequency of deleterious alleles inTNFAIP3was higher in the AIH subset without theDRB1risk alleles than that with (P = 0.0052, OR 5.10, 95%CI 1.55–16.74). The deleterious alleles inTNFAIP3were associated with AIH with cirrhosis.
Elevated microRNA-145 inhibits the development of oral squamous cell carcinoma through inactivating ERK/MAPK signaling pathway by down-regulating HOXA1
Authors: Ding Junhai , Sun Dubin , Xie Pengfeng 
Published: May 2019

Abstract
Background: Oral cancer is one of the most frequent solid cancers worldwide, and oral squamous cell carcinoma (OSCC) constitutes approximately 90% of oral cancers. The discovery of reliable prognostic indicators would be a potential strategy for OSCC treatment. In the present study, we aim to explore the underlying mechanism by which microRNA-145 (miR-145) affected OSCC. Methods: Forty-eight patients diagnosed with OSCC were enrolled to obtain the OSCC tissues and adjacent normal tissues. The targeting relationship between miR-145 and Homeobox A1 (HOXA1) was verified. In order to assess the effects of miR-145 in OSCC and the detailed regulatory mechanism, the SCC-9 cell line was adopted, in which expression of miR-145 and HOXA1 were altered by transfection. Then, a series of in vitro and in vivo experiments were performed to evaluate the cell viability, migration, invasion, and tumor growth. Results: miR-145 was poorly expressed and HOXA1 was highly expressed in OSCC. HOXA1 was verified as a target of miR-145 to mediate the activation of the extracellular signal-regulated kinase/mitogen activated protein kinase (ERK/MAPK) signaling pathway. In the circumstance of miR-145 elevation or HOXA1 depletion, the SCC-9 cell line manifested with inhibited cell viability, invasion, and migration in vitro, coupled with reduced tumor growth in vivo, with a decreased expression of ERK/MAPK signaling pathway-related genes/proteins. Conclusion: These findings suggested that miR-145 can inhibit HOXA1 to inactivate the ERK/MAPK signaling pathway, thereby suppressing OSCC cell proliferation, migration, and invasion to further inhibit the development of OSCC, highlighting a novel therapeutic target for the OSCC treatment.
Yi-qi-yang-yin-tian-sui-fang enhances cisplatin-induced tumor eradication and inhibits interleukin-7 reduction in non-small cell lung cancer
Authors: Ke Bin , Wu Xinlin , Yang Qiong , Huang Yuanyuan , Wang Fang , Gong Yuxin , Liu Junfang , Shi Lin 
Published: May 2019

Abstract
Traditional Chinese Medicine (TCM) has been recognized to be conducive to enhancing the efficiency and reducing the side effects in the whole course of cancer treatment. The mechanisms of TCM/chemotherapy combination involved with interleukin-7 (IL-7) potentially enhance immune responses against tumor. In the present study, we emphasized on a herbal formulation Yi-qi-yang-yin-tian-sui-fang or TCM for short, and investigated its roles in chemotherapy in non-small cell lung cancer (NSCLC). The mice bared with tumor were treated with cisplatin (DDP) and simultaneously administrated with/without low, medium and high doses of TCMs (effective content: 0.5, 2.0 and 8.0 g/per mice) via oral gavage. The results indicated that combination of TCM further elevated the therapy efficiency of DDP in a dose-dependent manner. The growth of tumor cells was estimated by Ki-67 stain and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) assay. The addition of TCM to the DDP treatment could significantly decrease the expression of Ki-67 and promote the apoptosis of tumor cells. In addition, the serum IL-7 level was down-regulated by DDP but restored by the treatment of TCM. The expression of IL-7 and its receptor IL-7R in tumor tissues was also recovered by TCM. Furthermore, the side effect from bone marrow suppression (myelosuppression) induced by DDP were assessed. TCM could abrogate DDP-induced apoptosis of bone marrow and also remarkably induced the expressions of IL-7 and hematopoietic growth factors including G-CSF, GM-CSF, SCF, and SDF-1 in bone marrow. These data indicated that this TCM combined with DDP showed superior anti-tumor effects with reduced myelosuppression via up-regulating IL-7.
Malondialdehyde on postoperative day 1 predicts postoperative cognitive dysfunction in elderly patients after hip fracture surgery
Authors: Wu Chunxian , Gao Bin , Gui Yu 
Published: May 2019

Abstract
Background: Postoperative cognitive dysfunction (POCD) is a great problem for anesthetized subjects and is associated with poor short- and long-term outcomes. We explored promising predictors for POCD in elderly patients after hip fracture surgery.
Methods: Elderly subjects (aged ≥65 years) undergoing surgery for hip fracture were consecutively recruited. Neuropsychological assessments were performed 1 day preoperatively (baseline) and 7 days postoperatively, and POCD was defined using the ‘Z scores’ method. Clinical data and laboratory tests were compared between patients with and without POCD development. Binary univariate and multivariate logistic regression analyses were conducted for risk factor assessment. Receiver operating characteristic (ROC) curve analysis was performed to investigate the predictive value of malondialdehyde (MDA) on postoperative day 1 (POD1) for POCD.
Results: A total of 198 patients were finally enrolled in the analysis and 51 patients exhibited POCD within 7 postoperative days, with an incidence rate of 25.8%. MDA expression on POD1 (OR: 1.12, 95%CI: 1.03–1.23, P=0.017) was the only independent risk factor for POCD according to the final multivariate logistic regression analysis. ROC curve analysis indicated that MDA on POD1 was a predictor for POCD, with an area under the curve (AUC) of 0.683 and 95%CI of 0.590–0.775 (P&lt;0.001).
Conclusions: In conclusion, we demonstrated that MDA on POD1 was an independent risk factor for POCD in elderly subjects undergoing hip fracture surgery.
Aqueous humour concentrations of PEDF and Erythropoietin are not influenced by subthreshold micropulse laser treatment of diabetic macular edema
Authors: Midena Edoardo , Bini Silvia , Frizziero Luisa , Pilotto Elisabetta , Esposito Graziana , Micera Alessandra 
Published: May 2019

Abstract
Purpose: To determine if aqueous humour (AH) concentrations of Retinal Pigment Epithelium (RPE)’s biomarkers are modified after subthreshold micropulse laser (SMPL) treatment of diabetic macular edema (DME).
Methods: Naïve DME and healthy subjects were enrolled. All DME patients received SMPL treatments (577-nm yellow light, 5% duty cycle of 0.2 s, power 250 mW), according to study protocol. AH of DME eyes was sampled at baseline and periodically after first SMPL treatment. Control eyes were sampled before cataract surgery. Pigment Epithelium Derived Factor (PEDF) and Erythropoietin (EPO) were quantified with glass-chip protein array.
Results: Eighteen DME patients (central retinal thickness ≤ 400 μm on Spectral Domain Optical Coherence Tomography (SD-OCT)) and ten controls were enrolled. The main exclusion criteria were high refractive error, proliferative diabetic retinopathy, glaucoma and neurodegenerative disorders. PEDF concentration was decreased in DME patients at baseline versus controls (P=0.012), while EPO was increased (P=0.029). Both molecules’ concentrations remained stable during follow-up after treatments, compared with DME-baseline.
Conclusions: The AH concentrations of RPE biomarkers were significantly different in DME treatment-naïve eyes versus controls. The expression of PEDF and EPO remained unchanged after treatments with SMPL in DME eyes. These data are relevant for future research and applications of SMPL.
Catechol cross-linked antimicrobial peptide hydrogels prevent multidrug-resistant Acinetobacter baumannii infection in burn wounds
Authors: Khan Abidullah , Xu Miao , Wang Tengjiao , You Chuangang , Wang Xingang , Ren Haitao , Zhou Hongwei , Khan Amin , Han Chunmao , Li Peng 
Published: May 2019

Abstract
Hospital-acquired infections are common in burn patients and are the major contributors of morbidity and mortality. Bacterial infections such as Staphylococcus aureus (S. aureus) and Acinetobacter baumannii (A. baumannii) are difficult to treat due to their biofilm formation and rapidly acquiring resistance to antibiotics. This work presents a newly developed hydrogel that has the potential for treating bacterial wound infections. The hydrogel formulation is based on an antimicrobial peptide (AMP), epsilon-poly-l-lysine (EPL) and catechol, which was cross-linked via mussel-inspired chemistry between the amine and phenol groups. In vitro studies showed that EPL-catechol hydrogels possess impressive antimicrobial and antibiofilm properties toward multidrug-resistant A. baumannii (MRAB). In addition, cytotoxicity study with the clonal mouse myoblast cell line (C2C12) revealed the good biocompatibility of this hydrogel. Furthermore, we created a second-degree burn wound on the mice dorsal skin surface followed by contamination with MRAB. Our results showed that the hydrogel significantly reduced the bacterial burden by more than four orders of magnitude in infected burn wounds. Additionally, there was no significant histological alteration with hydrogel application on mice skin. Based on these results, we concluded that EPL-catechol hydrogel is a promising future biomaterial to fight against multidrug-resistant bacterial infections.
Wave modes excited by photosphericp-modes and mode conversion in a multi-loop system
Authors: Riedl J. M. , Van Doorsselaere T. , Santamaria I. C. 
Published: May 2019

Context. Waves are ubiquitous in the solar corona and there are indications that they are excited by photosphericp-modes. However, it is unclear howp-modes in coronal loops are converted to sausage modes and transverse (kink) modes, which are observed in the corona.Aims. We aim to investigate how those wave modes are excited in the lower corona by photospheric acoustic waves.Methods. We built 3D magnetohydrostatic loop systems with multiple inclinations spanning from the photosphere to the lower corona. We then simulated these atmospheres with the MANCHA code, in which we perturb the equilibrium with ap-mode driver at the bottom of the domain. By splitting the velocity perturbation into components longitudinal, normal, and azimuthal to the magnetic flux surfaces we can study wave behavior.Results. In vertical flux tubes, we find that deformed fast sausage surface waves and slow sausage body waves are excited. In inclined flux tubes fast kink surface waves, slow sausage body waves, and either a fast sausage surface wave or a plane wave are excited. In addition, we calculate a wave conversion factor (0 ≤ C ≤ 1) from acoustic to magnetic wave behavior by taking the ratio of the mean magnetic energy flux to the sum of the mean magnetic and acoustic energy flux and compare it to a commonly used theoretical conversion factor. We find that between magnetic field inclinations of 10° to 30° those two methods lie within 40%. For smaller inclinations the absolute deviation is smaller than 0.1.
Ambulante Versorgungsqualität für den permanenten postoperativen Hypoparathyreoidismus
Authors: Allemeyer Erik Hermann , Kossow Marielle Sophie , Riemann Burkhard , Hoffmann Matthias Wilhelm 
Published: May 2019

Zusammenfassung
Einleitung Der postoperative Hypoparathyreoidismus kann erhebliche akute Symptome, relevante Folgeerkrankungen und weitreichende Auswirkungen auf die Lebensqualität verursachen. Die sozioökonomische Bedeutung ist hoch. Eine individualisierte Nachsorge mit besonderer Berücksichtigung gefährdeter Patienten könnte die Versorgungsqualität verbessern. Am eigenen Patientengut unseres Schilddrüsenzentrums (Kompetenzzentrum DGAV) analysierten wir Versorgungsstrukturen und -qualität und zeigen Verbesserungspotenziale auf.
Material und Methoden Konsekutive Serie von 420 Patienten mit totaler Thyreoidektomie im Zeitraum 08/2012 bis 08/2014, Follow-Up 8–32 Monate. Gruppe I (Untersuchungskollektiv): 197 Patienten mit Calcium (Ca) im Serum (i. S.) am 1. postop. Tag < 2,1 mmol/l und/oder Parathormon (PTH) < 15 pg/ml. Gruppe II (Kontrollkollektiv): 223 Patienten mit Ca i. S. am 1. postop. Tag ≥ 2,1 mmol/l und PTH ≥ 15 pg/ml. Zielparameter bei Follow-Up: Ca- und PTH-Wert, Symptome, Lebensqualität und ambulante Versorgungsqualität durch Hausarzt (Fragebogen).
Ergebnisse Teilnahmequote bei der Follow-Up-Untersuchung 30,6 % (117/382). Häufigkeiten für Hypoparathyreoidismus: 47 % passager, 6 % permanent. Symptomzahl und empfundene Belastung bei Patienten mit Hypoparathyreoidismus nahezu identisch zum Vergleichskollektiv. Nachsorge erfolgte bei 96 % durch den Hausarzt. Kein Patient mit einem Serumcalcium ≤ 2,1 mmol/l in der Follow-Up-Untersuchung wurde nicht substituiert. Bei 33 % der substituierten Pat. mit permanentem Hypoparathyreoidismus war die Substitutionstherapie nicht ausreichend. 24 % aller 110 Patienten mit normwertigem PTH in der Follow-Up-Untersuchung erhielten eine Substitutionsmedikation trotz überwiegend (76 %) regelmäßiger Ca-Kontrollen beim Hausarzt.
Schlussfolgerung In der ambulanten hausärztlichen Versorgung von Patienten mit Hypoparathyreoidismus stellten wir sowohl Merkmale einer Über- als auch einer Unterversorgung fest. Hieraus lassen sich zur Verbesserung Zielparameter ableiten: 1. Engmaschige Nachsorge, 2. Ausreichende Substitutionstherapie für alle Patienten mit einem permanenten Hypoparathyreoidismus, 3. Zeitgerechte Beendigung der Substitutionstherapie für alle Patienten mit normwertigem Parathormon.
Acute Pulmonary Embolism: Imaging Techniques, Findings, Endovascular Treatment and Differential Diagnoses
Authors: Palm Viktoria , Rengier Fabian , Rajiah Prabhakar , Heussel Claus Peter , Partovi Sasan 
Published: May 2019

Background Acute thrombotic pulmonary embolism (PE) is a common and potentially fatal event with imaging playing a pivotal role in the diagnosis and management of these patients.
Method This review discusses imaging techniques, diagnostic algorithms, imaging findings and endovascular treatment of acute thrombotic PE, and illustrates important differential diagnoses relating to the spectrum of acute non-thrombotic PE and non-embolic pulmonary artery disease. The review emphasizes information relevant for everyday radiological practice and highlights recent advances that can be readily applied in the clinical routine.
Results/Conclusion Computed tomography pulmonary angiography (CTPA) is the current reference standard for the diagnosis of acute PE. Ventilation and perfusion (VQ) scanning or – in centers with adequate expertise – magnetic resonance imaging (MRI) is indicated in pregnant or young patients and patients with contraindications to iodinated contrast. Invasive angiography is reserved for patients with intended endovascular treatment. Artifacts, acute non-thrombotic PE, chronic PE and non-embolic pulmonary artery diseases should always be considered as differential diagnoses.
Key points:
Citation Format
Experimental 177Lu-PSMA-617 radioligand therapy in a patient with extended metastasized leiomyosarcoma
Authors: Jüptner Michael , Marx Marlies , Zuhayra Maaz , Lützen Ulf 
Published: May 2019

IntroductionSystemic radionuclide therapy with 177Lu-PSMA-617 is a novel treatment option in patients with metastasized and castration-resistant prostate cancer 4. The molecular target of the 177Lu-PSMA-617 radioligand therapy is the prostate-specific membrane antigen (PSMA) highly expressed on prostate cancer cells. Beyond the enhanced a﻿ccumulation of PSMA on prostate cancer cells, PSMA expression is also found on the molecular surface or in the tumor-associated neovasculature of various tumor tissues including sarcomas of the soft tissue 2. Thus, PSMA has theoretically been discussed as a possible future target for systemic radioligand therapy with 177Lu-PSMA-617 even in non-prostate malignancies 1.Here we report on a female patient with extended metastasized leiomyosarcoma experimentally treated with one application of 177Lu-PSMA-617 radioligand therapy.