bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of multiple thawing techniques on thaw time and stability of hemostatic proteins in canine plasma products
Authors: Torkildsen L. , Bishop M. A. , Barr J. W. , Pashmakova M. B. 
Published: July 2018

ObjectivesTo compare thawing times of fresh frozen canine plasma between a 37 °C warm water bath, running water bath and dry plasma thawer and compare haemostatic protein stability after thawing in a warm water bath or dry plasma thawer.Materials and MethodsTo measure thawing times, a 240‐mL bag of frozen plasma was thawed in warm water bath, running water bath or dry plasma thawer‐10 times for each method. To evaluate stability of haemostatic proteins, fresh canine donor plasma samples were split into 120‐mL bags and 3‐mL control aliquots before freezing. Bags were thawed by warm water bath or dry plasma thawer and aliquots equilibrated to room temperature. Concentrations of haemostatic proteins, albumin, D‐dimers, prothrombin time and partial thromboplastin time were obtained.ResultsThe running water bath had the shortest thaw time: median thaw time of 15 minutes versus 18 minutes for both the dry plasma thawer and warm water bath. Statistically significant differences in partial thromboplastin time, factor VII, factor X, von Willebrand factor, and von Willebrand factor collagen binding assay were detected among groups but were unlikely to be clinically relevant.Clinical SignificanceA traditional running water bath provided the fastest thawing time but the dry plasma thawer resulted in the most stable haemostatic proteins.
Macrobenthic infaunal communities associated with deep‐sea hydrocarbon seeps in the northern Gulf of Mexico
Authors: Washburn Travis W. , Demopoulos Amanda W. J. , Montagna Paul A. 
Published: July 2018

AbstractThere are thousands of seeps in the deep ocean worldwide; however, many questions remain about their contributions to global biodiversity and the surrounding deep‐sea environment. In addition to being globally distributed, seeps provide several benefits to humans such as unique habitats, organisms with novel genes, and carbon regulation. The purpose of this study is to determine whether there are unique seep macrobenthic assemblages, by comparing seep and nonseep environments, different seep habitats, and seeps at different depths and locations. Infaunal community composition, diversity, and abundance were examined between seep and nonseep background environments and among three seep habitats (i.e., microbial mats, tubeworms, and soft‐bottom seeps). Abundances were higher at seep sites compared to background areas. Abundance and diversity also differed among microbial mat, tubeworm, and soft‐bottom seep habitats. Although seeps contained different macrobenthic assemblages than nonseep areas, infaunal communities were also generally unique for each seep. Variability was 75% greater within communities near seeps compared to communities in background areas. Thus, high variability in community structure characterized seep communities rather than specific taxa. The lack of similarity among seep sites supports the idea that there are no specific infauna that can be used as indicators of seepage throughout the northern Gulf of Mexico, at least at higher taxonomic levels.
Escherichia coli and Pseudomonas aeruginosa lipopolysaccharide O‐antigen ligases share similar membrane topology and biochemical properties
Authors: Ruan Xiang , Monjarás Feria Julia , Hamad Mohamad , Valvano Miguel A. 
Published: July 2018

SummaryWaaL is an inner membrane glycosyltransferase that catalyzes the transfer of O‐antigen polysaccharide from its lipid‐linked intermediate to a terminal sugar of the lipid A‐core oligosaccharide, a conserved step in lipopolysaccharide biosynthesis. Ligation occurs at the periplasmic side of the bacterial cell membrane, suggesting the catalytic region of WaaL faces the periplasm. Establishing the membrane topology of the WaaL protein family will enable understanding its mechanism and exploit it as a potential antimicrobial target. Applying oxidative labeling of native methionine/cysteine residues, we previously validated a topological model for Escherichia coli WaaL, which differs substantially from the reported topology of the Pseudomonas aeruginosa WaaL, derived from the analysis of truncated protein reporter fusions. Here, we examined the topology of intact E. coli and P. aeruginosa WaaL proteins by labeling engineered cysteine residues with the membrane‐impermeable sulfhydryl reagent polyethylene glycol maleimide (PEG‐Mal). The accessibility of PEG‐Mal to targeted engineered cysteine residues in both E. coli and P. aeruginosa WaaL proteins demonstrates that both ligases share similar membrane topology. Further, we also demonstrate that P. aeruginosa WaaL shares similar functional properties with E. coli WaaL and that E. coli WaaL may adopt a functional dimer conformation.
The Theologian as Critic: The Contribution of Tinyiko Sam Maluleke
Authors: Ngong David Tonghou 
Published: July 2018

AbstractTinyiko Sam Maluleke is a prolific South African theologian whose work mounts a thoroughgoing critique of the status of Christianity in contemporary sub‐Saharan Africa. Situating his work within its archival context, this article argues that the work of Maluleke emphasizes the vocation of the theologian as critic. The essay looks at some key themes in his work and suggests that Maluleke's critique of African Christianity is soteriological in nature, that is, he questions the role the Christian faith may play in the salvation of (South) Africa.
Banana blossom agar (BABA), a new medium to isolate members of the Cryptococcus neoformans/Cryptococcus gattii species complex useful for resource limited countries
Authors: Khayhan Kantarawee , Juntaboon Siriprapa , Fang Wenjie , Chaowasku Tanawat , Amornthipayawong Dechphipat , Boekhout Teun 
Published: July 2018

SummaryIsolation of representatives of the Cryptococcus neoformans/Cryptococcus gattii species complex can be made using dopamine containing media, such as Niger seed agar and l‐DOPA agar. Here, we describe an alternative medium that uses banana flowers. Banana is a dopamine containing fruit and is widely available in tropical and subtropical countries that have high numbers of cryptococcosis patients. This banana blossom‐based agar is useful for the enrichment of isolates of the C. neoformans/C. gattii species complex from environmental and clinical materials. The banana blossom agar (BABA) with and without creatinine can differentiate between the melanin forming isolates of the C. neoformans/C. gattii species complex from other yeasts that do not form melanin.
A Thomistic Model of Friendship with God as Deification
Authors: Christianson Raphael Joshua 
Published: July 2018

AbstractWhile friendship with God is an important theme for Thomas Aquinas, Aquinas never explicitly delineates what such friendship looks like. In this article, I present a systematic description of friendship with God according to Thomas Aquinas. I examine three dynamics (mutuality, benevolence, and communicatio) and two effects (mutual indwelling and union) which Aquinas attributes to human friendship, and I show how they can exist analogically in friendship with God. Such a presentation reveals that friendship with God effects the deification of the human person, and such a deified condition is the intended state of the human person, in which he or she can be most fully human. This conclusion allows me to propose a way to define a human person as a relation (using the term deificatus), analogous to Aquinas's definition of person for the Trinity. In turn, I show how this reality impacts a Thomistic understanding of genuine human friendship, whereby one friend loves the other qua deificatus.
Not Crying “Peace” The Theological Politics of Herbert McCabe
Authors: Hewitt Simon 
Published: July 2018

AbstractHerbert McCabe was, by widespread acclaim, one of the greatest Catholic thinkers in the English speaking world during the final quarter of the last century. He was also deeply committed to radical left‐wing politics. What is the relationship between these two facts? I lay out what I take to be the key themes in McCabe's politics before arguing that, in contrast to significant strands in present day political theology, he had a keen sense of the respective roles of faith and reason in guiding political action. This allowed him to commit himself to a politics which was more radical than much of what has followed him, whilst having a clear sense of how Christian faith ought to be allowed to condition political engagement. The article concludes by drawing out lessons from McCabe for thinking about faith and politics. Not least of these is that we ought to avoid the lure, often articulated in terms of the ‘unifying’ role of the Church, to eschew conflict.
Can we distinguish three maintenance processes in working memory?
Authors: Morey Candice C. , Cowan Nelson 
Published: July 2018

AbstractWe describe three mechanisms—consolidation, refreshing, and removal—as processes that may serve to strengthen new memories. We detail their explicit and implied differences and similarities, and highlight points upon which theorists disagree about their supposed characteristics. We consider the challenges remaining in refining definitions of these processes and with situating them within working memory theories, and consider how these process definitions and theories should restrict each other.
Plasma membrane H+‐ATPase activity and graft success of breadfruit (Artocarpus altilis) onto interspecific rootstocks of marang (A. odoratissimus) and pedalai (A. sericicarpus)
Authors: Zhou Y. , Underhill S. J. R. 
Published: July 2018

Abstract

Breadfruit (Artocarpus altilis) is primarily grown as a staple tree crop for food security in Oceania. Significant wind damage has driven interest in developing its dwarfing rootstocks. Due to the predominantly vegetative propagation of the species, grafting onto interspecific seedlings is an approach to identifying dwarfing rootstocks. However, grafting of breadfruit onto unrelated Artocarpus species has not been investigated.

Here we first report the success of breadfruit grafting onto interspecific rootstocks, marang (A. odoratissimus) and pedalai (A. sericicarpus). To address the low graft survival, we investigated the relationship of plasma membrane (PM) H+‐ATPase activity to graft success.

We provide the first evidence for a positive correlation between PM H+‐ATPase activity and graft survival. The graft unions of successful grafts had higher PM H+‐ATPase activity compared to those of failed grafts. Rootstocks with low PM H+‐ATPase activity in leaf microsomes before grafting had lower graft survival than those with high enzyme activity, with graft success of 10% versus 60% and 0% versus 30% for marang and pedalai rootstocks, respectively. There was a positive correlation between graft success and the PM H+‐ATPase activity measured from the rootstock stem microsomes 2 months after grafting [marang, r(7) = 0.9203, P = 0.0004; pedalai (r(7) = 0. 8820, P = 0.0017]. Removal of scion's own roots decreased the leaf PM H+‐ATPase activity of grafted plants regardless of the final graft outcome. Recovery of the enzyme activity was only found in the successful grafts.

The function of PM H+‐ATPase in graft union development and graft success improvement is discussed.
Robust Sourcing from Suppliers under Ambiguously Correlated Major Disruption Risks
Authors: Zhao Ming , Freeman Nickolas K. 
Published: July 2018

As the severity and frequency of supply chain disruptions increases due to globalization and outsourcing, companies are faced with the challenge of managing correlated disruption risks. However, correctly estimating supplier correlations is difficult and relying on incorrect estimates can be costly. Motivated by these challenges, we consider models for managing disruption risks when suppliers are ambiguously correlated. Our models are based on profit‐oriented correlation structures that only require accurate estimates of the marginal disruption probabilities for available suppliers. Analysis of the models, along with extensive numerical studies, show that they offer high‐quality solutions and provide several important insights regarding supplier selection, appropriate budgets for efforts to better understand the prevailing correlation structure, and identifying subsets of the available suppliers that should be considered for strategic alliances. In addition to these benefits, we discuss a progressive, profit‐oriented approach for risk management that is based on these models and is easily implemented in situations with minimal in formation regarding supplier correlations.
Assessment of photosynthetic performance in the two life history stages of Alaria crassifolia (Laminariales, Phaeophyceae)
Authors: Borlongan Iris Ann , Nishihara Gregory N. , Shimada Satoshi , Terada Ryuta 
Published: July 2018

SUMMARYUnderstanding of the physiological responses of kelp to environmental parameters is crucial, especially in the context of environmental change that may have contributed to the decline of kelp forests all over the world. The current study presents the photosynthetic characteristics of the macroscopic sporophyte and microscopic gametophyte stages of the brown alga Alaria crassifolia from Hokkaido, Japan, as determined by examining their photosynthetic responses over a range of temperature and irradiance using dissolved oxygen and chlorophyll fluorescence measurements. Net photosynthetic rates of the sporophyte were consistently higher than those of gametophyte across temperature gradients and irradiance levels. Photosynthesis–irradiance curves at 8°C, 16°C, and 20°C revealed similar initial slopes (α = 0.4–0.9) on the two life history stages, but higher compensation (E
c = 4–7 μmol photons m−2 s−1) and saturation irradiances (E
k = 53–103 μmol photons m−2 s−1) for the sporophyte than for the gametophyte (E
c = 0–7 μmol photons m−2 s−1; E
k = 7–10 μmol photons m−2 s−1). Both stages exhibited chronic photoinhibition, as shown by the failure of recovery in their maximum quantum yields (F
v/F
m) following high irradiance stress, with greater possibility of photodamage at low temperature. Gametophytes were less sensitive to low temperatures than sporophytes, given their relatively stable F
v/F
m response. Nevertheless, temperature optima for photosynthesis of both stages coincide with each other at 20–23°C, which correspond to the growth and maturation periods of A. crassifolia in Japan. This species is also likely to suffer from thermal inhibition as both GP rates and F
v/F
m decreased above 24°C.
Taking a Closer Look at the Empowerment‐Performance Relationship: Evidence from Law Enforcement Organizations
Authors: Hassan Shahidul , Park Jongsoo , Raadschelders Jos C. N. 
Published: July 2018

AbstractThis article examines the influence of empowering leadership practices on police officers' job performance, perceptions of managerial effectiveness, and unit performance. These relationships are examined using multisource survey data collected from 100 law enforcement managers, 446 of their subordinates, and 98 of their direct supervisors. The analysis shows that empowering leadership contributes positively to subordinate officers' job performance and unit effectiveness. Empowering leadership is also positively associated with subordinate but not with supervisor ratings of managerial effectiveness. Task‐oriented leadership, however, is positively associated with both subordinate and supervisor ratings of managerial effectiveness. Implications of these results for managerial leadership in law enforcement organizations are discussed.
Cardiovascular adaptations after 10 months of intense school‐based physical training for 8‐ to 10‐year‐old children
Authors: Larsen Malte Nejst , Nielsen Claus Malta , Madsen Mads , Manniche Vibeke , Hansen Lone , Bangsbo Jens , Krustrup Peter , Hansen Peter Riis 
Published: July 2018

This study examined cardiovascular adaptations in 8‐ to 10‐year‐old schoolchildren after 10 months (a full school year) of 3 × 40 minute per week of small‐sided ball games (SSG, including football, basketball, and/or floorball) or circuit strength training (CST). The study involved 291 Danish schoolchildren, 8‐10 years old, cluster‐randomized to SSG (n = 93, 4 schools, 5 classes), CST (n = 83, 4 schools, 4 classes), or a control group (CON, n = 115, 2 schools, 5 classes). Before and after the 10‐month intervention, resting heart rate and blood pressure measurements were performed as well as comprehensive transthoracic echocardiography and peripheral arterial tonometry (PAT). Analysis of baseline‐to‐10‐months changes showed between‐group differences (P < 0.05) after both training interventions in diastolic blood pressure (delta scores: SSG −2.1 ± 6.0 mm Hg; CST −3.0 ± 7.1 mm Hg; CON 0.2 ± 5.3 mm Hg). Moreover, there were between‐group differences in delta scores (P < 0.05) in interventricular septum thickness (SSG 0.17 ± 0.87 mm; CST 0.30 ± 0.94 mm; CON −0.15 ± 0.68 mm), left‐atrial volume index (SSG 0.32 ± 5.13 mL/m2; CON 2.60 ± 5.94 mL/m2), and tricuspid annular plane systolic excursion (SSG −0.4 ± 3.3 mm; CON: 0.1 ± 3.6 mm). No significant between‐group differences were observed for the PAT‐derived reactive hyperemia index. In conclusion, 10 months of 3 × 40 minutes per week of SSG and CST in 8‐ to 10‐year‐old children decreased diastolic blood pressure and elicited discrete cardiac adaptations, suggesting that intense physical exercise in school classes can have effects on cardiovascular health in children.
Transcriptomics analyses reveal the molecular roadmap and long non‐coding RNA landscape of sperm cell lineage development
Authors: Liu Lingtong , Lu Yunlong , Wei Liqin , Yu Hua , Cao Yinghao , Li Yan , Yang Ning , Song Yunyun , Liang Chengzhi , Wang Tai 
Published: July 2018

SummarySperm cell (SC) lineage development from the haploid microspore to SCs represents a unique biological process in which the microspore generates a larger vegetative cell (VC) and a smaller generative cell (GC) enclosed in the VC, then the GC further develops to functionally specified SCs in the VC for double fertilization. Understanding the mechanisms of SC lineage development remains a critical goal in plant biology. We isolated individual cells of the three cell types, and characterized the genome‐wide atlas of long non‐coding (lnc) RNAs and mRNAs of haploid SC lineage cells. Sperm cell lineage development involves global repression of genes for pluripotency, somatic development and metabolism following asymmetric microspore division and coordinated upregulation of GC/SC preferential genes. This process is accompanied by progressive loss of the active marks H3K4me3 and H3K9ac, and accumulation of the repressive methylation mark H3K9. The SC lineage has a higher ratio of lncRNAs to mRNAs and preferentially expresses a larger percentage of lncRNAs than does the non‐SC lineage. A co‐expression network showed that the largest set of lncRNAs in these nodes, with more than 100 links, are GC‐preferential, and a small proportion of lncRNAs co‐express with their neighboring genes. Single molecular fluorescence in situ hybridization showed that several candidate genes may be markers distinguishing the three cell types of the SC lineage. Our findings reveal the molecular programming and potential roles of lncRNAs in SC lineage development.
Tense and the other: Temporality and urban multiculture in Auckland, Aotearoa New Zealand
Authors: Higgins Katie W. 
Published: July 2018

Expressions of concern about the national future, or the surfacing of history through postcolonial melancholia and nostalgia for a lost Golden Age, illustrate how temporality and tense have been absorbed into discourses, affective attachments and practices of cultural recognition and national belonging. First, this paper aims to develop the discussion of urban multiculture in human geography in an original direction through a theoretically‐driven argument for the significance of social divisions of tense. It contributes new knowledge about the availability of this discursive field when the issue of cultural recognition arises. Second, through considering the relational dynamics between settler, Indigenous and exogenous peoples together, the paper ties together debate on migration and ethnicity with indigeneity and colonialism. Third, the paper emphasises the importance of careful attention to local histories, contexts and oppressions when researching conviviality and multiculture in a settler colonial context. The analysis draws on 12 months of qualitative research with first‐generation British migrants in Auckland, Aotearoa New Zealand to examine several illustrative encounters of British migrants with Māori, the Indigenous peoples, and exogenous alterity, a term used to refer to migrants and racialised citizens deemed “foreign.”
Ventral cervical versus bilateral lateral approach for extirpation of mandibular and medial retropharyngeal lymph nodes in dogs
Authors: Wainberg S. H. , Oblak M. L. , Giuffrida M. A. 
Published: July 2018

AbstractObjectiveTo compare ventral cervical and bilateral lateral incisions for extirpation of mandibular and medial retropharyngeal lymph nodes in dogs.Study designProspective randomized, crossover controlled cadaver trial.Sample populationEight veterinarians with advanced surgical training.MethodsStudy participants were randomized to perform both techniques on paired cadavers. Time to extirpation of the first and last lymph node, length of incisions, and complications were recorded for both techniques. Participants were asked to rate satisfaction with their ability to identify local anatomy and lymph nodes as well as overall preferred technique by using a 10‐point numerical rating scale.ResultsThe total length of skin incised for the bilateral lateral approach exceeded that of the ventral cervical approach by 52.1 mm (mean, P < .001). The surgical time for removal of all 4 lymph nodes did not differ between the 2 approaches. The bilateral lateral approach was preferred by 62.5% (5/8) of participants for visualization of mandibular lymph nodes, and the ventral cervical approach was preferred by 87.5% (7/8) of participants for visualization of local anatomy. Overall, 62.5% (5/8) preferred the ventral cervical approach and 37.5% (3/8) preferred the bilateral lateral approach.ConclusionThe ventral cervical approach was preferred by participants for its perceived superior visualization of local anatomy and access to lymph nodes for removal. This approach also resulted in an overall shorter incision length.Clinical significanceA ventral cervical or bilateral lateral approach allows successful removal of the medial mandibular and retropharyngeal lymph nodes in dogs, and surgical approach may be selected according to individual preference.