bluuebunny/crossref_metadata_2025_split huggingface dataset:
Large-scale production of Mansonella perstans infective larvae from engorged Culicoides milnei
Authors: Kien Chi Anizette , Ebai Rene , Fombad Fanny Fri , Esofi Frederick , Ntuh Anna Ning , Ouam Emmanuel , Gandjui Narcisse Victor Tchamatchoua , Chunda Valerine Chawa , Ekanya Relindis , Nietcho Franck Noel , Foyet Juluis Visnel , Nchang Lucy Cho , Magha Chefor , Njouendou Abdel Jelil , Enyong Peter , Hoerauf Achim , Ritter Manuel , Wanji Samuel 
Published: December 2024

BackgroundMansonella perstans is transmitted by Culicoides species and affects hundred millions of inhabitants in about 33 countries in sub-Saharan Africa. It is known that Mansonellosis due to Mansonella perstans do not result in a clear clinical picture, but down-regulates the immunity of patients predisposing them to other diseases like tuberculosis, HIV and malaria or damping vaccine efficacy. However, research about novel drugs against this filarial nematode is missing because of the lack of parasite material. Previous studies have developed in vitro culture systems using infective stage 3 larvae (L3), but these life stages are difficult to obtain and thus the performance of in vitro cultures is restricted and does not allow large-scale testing of drugs or even infection experiments in animal models. Therefore, we aim to establish a platform for the large-scale production of M. perstans infective larvae from engorged Culicoides milnei.MethodsCulicoides species were caught in Yangom (Yabassi Health District) in the Littoral Region of Cameroon following a blood meal on six microfilariae-positive donors with different microfilaraemic loads over one year. Engorged midges were reared in the insectarium for up to 14 days and L3 were isolated from the different body parts.ResultIn summary, 13,658 engorged Culicoides were collected and reared in the laboratory. We observed an overall predicted survival of 78.5%. Out of the 8,123 survived midges, 7,086 midges belong to C. milnei, from which 2,335 were infected leading to a recovery of 6,310 L3. Moreover, we found the highest survival rates of midges during the early dry season in December with moderate temperatures (23-25°C) and low (2-4mm) or no rainfall. In addition, we observed that midges that fed on donors with high microfilarial loads showed increased mortality.ConclusionWe revealed suitable conditions for the collection and maintenance of engorged Culicoides midges allowing the large-scale production of M. perstans L3. This procedure will provide a platform to produce sufficient parasite material that will facilitate in vitro cultures and the establishment of a murine model of M. perstans, which is important for in-depth investigation of the filarial biology and screening of novel drugs that are effective against this ivermectin-resistant nematode.
HDC downregulation induced by chronic stress promotes ovarian cancer progression via the IL-6/STAT3/S100A9 pathway
Authors: Chen Zhicong , Cao Jinming , Xiao Zhijun , Yang Zhen , Cheng Yuanchi , Duan Jingjing , Zhou Ting , Xu Feng 
Published: December 2024

ObjectiveThis study aimed to investigate the underlying mechanism of chronic stress promoting ovarian cancer growth comorbid with depression and evaluate the potential role of histamine (HIS) in treating this comorbidity.MethodsChronic unpredictable mild stress (CUMS) was used to establish a comorbid mouse model of ovarian cancer and depression. The behavioral phenotypes were assessed using the sucrose preference test (SPT), tail suspension test (TST), forced swimming test (FST), and open field test (OFT). Ovarian cancer growth was monitored by tracking the tumor volume and weight. Histidine decarboxylase (HDC) expression in the tumor tissue was analyzed using Western blot and qRT-PCR techniques. The serum levels of inflammatory factors (IL-6 and IL-17A), stress hormones (norepinephrine, NE and cortisol, and COR), histamine, and 5-hydroxytryptamine (5-HT) were detected by enzyme-linked immunosorbent assay (ELISA). In vitro experiments were conducted to explore the direct impacts of stress hormones on A2780 and ES-2 ovarian cancer cell lines, as well as the modulation of these effects by histamine. HDC knockdown and overexpression approaches were used to study its regulatory role in the IL-6/STAT3/S100A9 signaling pathway.ResultsChronic stress not only induced depressive behaviors but also accelerated ovarian cancer growth in mice by downregulating HDC expression in tumors, whereas exogenous HIS treatment alleviated depressive symptoms, suppressed cancer growth, and countered the decreased levels of HIS and increased levels of IL-6, IL-17A, NE, COR, and 5-HT induced by CUMS. Furthermore, HIS positively modulated the immune response by increasing the populations of CD3+T and CD8+ T cells and reducing IL-17A secretion. In vitro experiments revealed that stress hormones downregulated HDC expression, consequently promoting cancer cell proliferation, migration, and invasion via the IL-6/STAT3/S100A9 pathway. Knockdown of HDC activated this pathway, whereas HDC overexpression inhibited its activation.ConclusionChronic stress leads to the downregulation of HDC expression, thereby facilitating the progression of ovarian cancer through the IL-6/STAT3/S100A9 pathway. HIS might serve as a potential molecule for treating the comorbidities of ovarian cancer and depression.
Craving on the move: targeting smoking memories with a novel 3MDR–smoking cessation protocol
Authors: Koomen Annel P. , Kaag Anne Marije , Jongenelen Kees A. M. , Wouters Rianne , van Marle Hein J. F. , van den Heuvel Odile A. , van der Werf Ysbrand D. , De Vries Taco J. 
Published: December 2024

IntroductionImproved effectiveness and treatment adherence is needed in smoking cessation (SC) therapies. Another important challenge is to disrupt maladaptive drug-related memories. To achieve these goals, we developed a novel treatment strategy on the basis of motion-assisted memory desensitization and reprocessing (3MDR).MethodsIn this study, the added effect of a distractor task following memory recall during a newly designed 3-day SC version of 3MDR (3MDR-SC) protocol on reducing smoking cue–elicited craving was investigated in abstinent chronic smokers. Chronic smokers were randomly allocated to an active 3MDR-SC group (receiving 3MDR-SC with a working memory distractor task) (n = 42) or a control 3MDR-SC group (receiving 3MDR-SC with a non-distracting task) (n = 39). Smoking cue–induced craving and physiological measures were assessed at baseline (T0) and 1 day after the intervention (T4), and smoking behavior was measured at T0 and 2-week (FU1) and 3-month (FU2) follow-up.ResultsSignificant decreases in cue-induced craving from T0 to T4 and daily cigarette use from T0 to FU1 and FU2 were observed but not differ between the two experimental groups. Cue-induced changes in heart-rate variability and skin conductance, which did not differ from T0 to T4, and relapse at FU2 were also not different between groups. Dropout rate during intervention was 2.5%.ConclusionsThe 3-day 3MDR-SC intervention resulted in a reduction in cue-induced craving and smoking behavior and showed very good treatment adherence. There was no added effect of the distractor task on 3MDR-SC efficacy. Further studies, including a treatment as usual control, are needed to confirm 3MDR-SC as an effective SC therapy.
Isolasi dan Identifikasi Senyawa Metabolit Sekunder Fraksi Metanol pada Umbi Belajang Keissi' (Stephania venosa (Blume) Spreng)
Authors: Bonggi Nurlayla , Salempa Pince , Sulfikar Sulfikar 
Published: December 2024

ABSTRAKPenelitian eksplorasi ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa metabolit sekunder fraksi metanol pada Umbi Belajang Keissi’ (S. venosa). Metode yang digunakan terdiri dari prepasasi sampel, ekstraksi, fraksinasi, analisis Gas Cromatography and Mass Spectroscopy (GC-MS), fraksinasi lanjutan, pemurnian dan identifikasi dengan menggunakan Spektroskopi IR. Hasil uji fitokimia ekstrak metanol umbi S. venosa (Blume) Spreng positif mengandung alkaloid dan flavonoid. Fraksi H yang dianalisis menggunakan GC-MS mengandung senyawa Diisoktil ftalat yang merupakan senyawa golongan saponin dan senyawa lain seperti Roemerolin, (+)-Mecambroline, Krebanin, Disentrin, Berbin 2,3,9,10-tetrametoksi - (+)- (Tetrahidropalmatin), Guadiscidine, 11-Hidroksi akronin merupakan senyawa golongan alkaloid. Isolat H7 yang diperoleh berbentuk serbuk berwarna kuning dengan titik leleh 211oC. Data spektrum FTIR menunjukkan adanya gugus fungsi N-H sekunder, C-H Alifatik, C=C Aromatik, C-N, -CH2 dan -CH3. Berdasarkan data spektrum FTIR dan pengujian golongan di simpulkan senyawa yang diperoleh adalah senyawa golongan alkaloid.Kata Kunci: Stephania venosa, Alkaloid, Fraksi Metanol. ABSTRACTThis exploration study aims to isolate and identify secondary metabolite compounds of methanol fraction in Umbi Belajang Keissi’ (S. venosa). The methods used consist of sample preparation, extraction, fractionation, Gas Chromatography and Mass Spectroscopy (GC-MS) analysis, further fractionation, purification and identification using IR Spectroscopy.The results of phytochemical tests of methanol extract of S. venosa (Blume) Spreng tubers positively contained alkaloids and flavonoids. Fraction H analyzed using GC-MS contained Diisoctyl phthalate compounds which are saponin compounds and other compounds such as Roemerolin, (+)-Mecambroline, Crebanine, Dicentrin, Berbin 2,3,9,10-tetramethoxy - (+)- (Tetrahydropalmatin), Guadiscidine, 11-Hydroxy acronine are alkaloid compounds. The H7 isolate obtained was in the form of a yellow powder with a melting point of 211oC. FTIR spectrum data shows the presence of secondary N-H functional groups, Aliphatic C-H, Aromatic C=C, C-N, -CH2 and -CH3. Based on FTIR spectrum data and group testing, it is concluded that the compound obtained is an alkaloid compound.Keywords: Stephania venosa, Alkaloid, Methanol Fraction.
Study the effect of Cortisol resistance in patients with hypertension
Authors: Furqan Moein Auda  , Athraa Rehman  Kazem  
Published: December 2024

Background & objective: hypertension commonly interact, creating a difficult condition with major cardiovascular effects. Patients who have hypertension are more likely to experience their risk of cardiovascular disease (CVD) , left main disease involvement, and three-vessel
Methodology: sixty Patient with hypertention and thirty healthy are participated in this study. .Their ages ranged between 45-70 years. Samples were taken in the morning when they were fasting ,and it obtained from the Open Heart Institute, Al-Sadr Teaching Hospital, Najaf, Iraq .
Results: The study showed that patients with high blood pressure suffer from a significant increase in cortisol, low-density lipoprotein, cholesterol, and triglycerides, and a significant decrease in high-density lipoprotein, these markers are considered early indicators of heart disease .
Conclusions: From this study it be can concluded that patients with hypertention may have cortisol resistance, although patients continue to take treatment, the levels of, cholesterol, and low-density lipoprotein remain high in patients. On the other hand cortisol has high sensitive to predict heart disease in patients with hypertention .
Abbreviations: HDL-high density lipoprotein, LDL-low density lipoprote, TG-triglyceryde
Perbandingan Kombinasi Putih Telur Rebus dan Ikan Gabus dengan Pemberian Ikan Gabus Terhadap Penyembuhan Luka Sectio Cesarea pada Ibu Nifas di Rumah Sakit Ridhoka Salma Cikarang Barat
Authors: Galaupa Resi 
Published: December 2024

Latar Belakang : Angka Kematian Ibu (AKI) merupakan indikator strategis pembangunan kesehatan yang mencerminkan derajat kesehatan dan kualitas penduduk. Jumlah AKI di Indonesia pada tahun 2021 yang terhimpun dalam pencatatan profil kesehatan keluarga di Kemenkes RI masih menunjukan angka yang tinggi sebanyak 7.389 kematian (Kemenkes RI, 2022). Infeksi merupakan salah satu penyebab kematian ibu, di Negara berkembang paling sedikit satu dari sepuluh kematian ibu disebabkan oleh Infeksi. Luka pasca nifas masih menjadi kasus umum penyebab infeksi 80-90%. Infeksi setelah persalinan penyebabnya adalah luka persalinan normal atau sectio cesarea, mastitis, tromboflebitis dan radang panggul. Tujuan Penelitian : Mengetahui perbandingan kombinasi putih telur rebus dan ikan gabus dengan pemberian ikan gabus terhadap penyembuhan luka sectio cesarea pada ibu nifas Metode Penelitian : Desain penelitian ini yaitu penelitian kuantitatif jenis eksperimen (quasi eksperimen) dengan desain "pretest posttest one group desain. Sampel pada penelitian ini adalah seluruh ibu nifas dengan luka sectio cesarea di Rumah Sakit Ridhoka Salma Cikarang Barat pada bulan Desember 2023 – Januari 2024 sebanyak 30 responden. Hasil Penelitian : Berdasarkan hasil penelitian terdapat perbandingan dengan hasil uji T Test dengan nilai sig 0.02 < 0.05 yang berarti Ha Diterima : Terdapat perbandingan kombinasi putih telur rebus dan ikan gabus dengan pemberian ikan gabus terhadap penyembuhan luka sectio cesarea pada ibu nifas di Rumah Sakit Ridhoka Salma Tahun 2024. Kesimpulan Dan Saran : Terdapat perbandingan kombinasi putih telur rebus dan ikan gabus dengan pemberian ikan gabus terhadap penyembuhan luka sectio cesarea pada ibu nifas di Rumah Sakit Ridhoka Salma Tahun 2024. Diharapkan dari hasil penelitian ini dapat menjadi bahan masukan dan dapat diterapkan dilapangan untuk memberikan nutrisi hewani pada ibu nifas khususnya putih telur rebus dan ikan gabus untuk penyembuhan luka sectio cesarea
Cross-species real-time detection of trends in pupil size fluctuation
Authors: Kronemer Sharif I. , Gobo Victoria E. , Walsh Catherine R. , Teves Joshua B. , Burk Diana C. , Shahsavarani Somayeh , Gonzalez-Castillo Javier , Bandettini Peter A. 
Published: December 2024

Abstract
Pupillometry is a popular method because pupil size is easily measured and sensitive to central neural activity linked to behavior, cognition, emotion, and perception. Currently, there is no method for online monitoring phases of pupil size fluctuation. We introduce rtPupilPhase—an open-source software that automatically detects trends in pupil size in real time. This tool enables novel applications of real-time pupillometry for achieving numerous research and translational goals. We validated the performance of rtPupilPhase on human, rodent, and monkey pupil data, and we propose future implementations of real-time pupillometry.
Toward an Asian-based bodily movement database for emotional communication
Authors: Cheng Miao , Tseng Chia-huei , Fujiwara Ken , Higashiyama Shoi , Weng Abby , Kitamura Yoshifumi 
Published: December 2024

Abstract
Most current databases for bodily emotion expression are created in Western countries, resulting in culturally skewed representations. To address the obvious risk this bias poses to academic comprehension, we attempted to expand the current repertoire of human bodily emotions by recruiting Asian professional performers to wear whole-body suits with 57 retroreflective markers attached to major joints and body segments, and express seven basic emotions with whole-body movements in a motion-capture lab. For each emotion, actors performed three self-created scenarios that covered a broad range of real-life events to elicit the target emotion within 2–5 seconds. Subsequently, a separate group of participants was invited to judge the perceived emotional category from the extracted biological motions (point-light displays with 18 or 57 markers). The results demonstrated that the emotion discrimination accuracy was comparable to Western databases containing standardized performance scenarios. The results provide a significant step toward establishing a database using a novel emotional induction approach based on personalized scenarios. This database will contribute to a more comprehensive understanding of emotional expression across diverse contexts.
Searching for Planets Orbiting Vega with the James Webb Space Telescope
Authors: Beichman Charles , Bryden Geoffrey , Llop-Sayson Jorge , Ygouf Marie , Greenbaum Alexandra , Leisenring Jarron , Gaspar Andras , Krist John , Rieke George , Wolff Schuyler , Su Kate , Hodapp Klaus , Meyer Michael , Kelly Doug , Boyer Martha , Johnstone Doug , Horner Scott , Rieke Marcia 
Published: December 2024

Abstract
The most prominent of the IRAS debris disk systems, α Lyrae (Vega), at a distance of 7.7 pc, has been observed by both the NIRCam and MIRI instruments on the James Webb Space Telescope. This paper describes NIRCam coronagraphic observations, which have achieved F444W contrast levels of 3 × 10−7 at 1″ (7.7 au), 1 × 10−7 at 2″ (15 au), and a few × 10−8 beyond 5″ (38 au), corresponding to masses of <3, 2, and 0.5 M
J for a system age of 700 Myr. Two F444W objects are identified in the outer MIRI debris disk, around 48 au. One of these is detected by MIRI and appears to be extended with a spectral energy distribution similar to those of distant extragalactic sources. The second one also appears extended in the NIRCam data suggestive of an extragalactic nature. The NIRCam limits within the inner disk (1″–10″) correspond to model-dependent masses of 2–3 M
J. K. Y. L. Su et al. argue that planets larger than 0.3 M
J would disrupt the smooth disk structure seen at MIRI wavelengths. Eight additional objects are found within 60″ of Vega, but none have astrometric properties or colors consistent with planet candidates. These observations reach a level consistent with expected Jeans mass limits. Deeper observations achieving contrast levels < 10−8 outside of ∼4″ and reaching masses below that of Saturn are possible, but may not reveal a large population of new objects.
Kinematic Analysis of z = 4.3 Galaxies in the SPT2349–56 Protocluster Core
Authors: Venkateshwaran Aparna , Weiss Axel , Sulzenauer Nikolaus , Menten Karl , Aravena Manuel , Chapman Scott C. , Gonzalez Anthony , Gururajan Gayathri , Hayward Christopher C. , Hill Ryley , Reuter Cassie , Spilker Justin S. , Vieira Joaquin D. 
Published: December 2024

Abstract
SPT2349−56 is a protocluster discovered in the 2500 deg2 South Pole Telescope (SPT) survey. In this paper, we study the kinematics of the galaxies found in the core of SPT2349−56 using high-resolution (1.55 kpc spatial resolution at z = 4.303) redshifted [C ii] 158 μm data. Using the publicly available code
3D

BAROLO, we analyze the seven far-infrared brightest galaxies within the protocluster core. Based on conventional definitions for the detection of rotating disks, we classify six sources to be rotating disks in an actively star-forming protocluster environment, with weighted mean V
rot/σ
disp = 4.5 ± 1.3. The weighted mean rotation velocity (V
ro
t) and velocity dispersion (σ
disp) for the sample are 357.1 ± 114.7 km s−1 and 43.5 ± 23.5 km s−1, respectively. We also assess the disk stability of the galaxies and find a mean Toomre parameter of Q
T = 0.9 ± 0.3. The galaxies show a mild positive correlation between disk stability and dynamical support. Using the position–velocity maps, we find that five sources further classify as disturbed disks, and one classifies as a strictly rotating disk. Our sample joins several observations at similar redshift with high V/σ values, with the exception that they are morphologically disturbed, kinematically rotating, and interacting galaxies in an extreme protocluster environment.
Cross-correlation of Luminous Red Galaxies with Machine Learning Selected Active Galactic Nuclei in HSC-SSP: Unobscured AGN Residing in More Massive Halos
Authors: Córdova Rosado Rodrigo , Goulding Andy D. , Greene Jenny E. , Petter Grayson C. , Hickox Ryan C. , Kokron Nickolas , Strauss Michael A. , Givans Jahmour J. , Toba Yoshiki , Henderson Cassandra Starr 
Published: December 2024

Abstract
Active galactic nuclei (AGN) are the signposts of black hole growth, and likely play an important role in galaxy evolution. An outstanding question is whether AGN of different spectral types indicate different evolutionary stages in the coevolution of black holes and galaxies. We present the angular correlation function between an AGN sample selected from Hyper Suprime-Cam Subaru Strategic Program (HSC-SSP) optical photometry and Wide-field Infrared Survey Explorer mid-IR photometry and a luminous red galaxy (LRG) sample from HSC-SSP. We investigate AGN clustering strength as a function of luminosity and spectral features across three independent HSC fields totaling ∼600 deg2, for z ∈ 0.6 −1.2 and AGN with L
6 μm > 3 × 1044 erg s−1. There are ∼28,500 AGN and ∼1.5 million LRGs in our primary analysis. We determine the average halo mass for the full AGN sample (M

h
≈ 1012.9
h
−1
M
⊙), and note that it does not evolve significantly as a function of redshift (over this narrow range) or luminosity. We find that, on average, unobscured AGN (M

h
≈ 1013.3
h
−1
M
⊙) occupy ∼4.5× more massive halos than obscured AGN (M

h
≈ 1012.6
h
−1
M
⊙), at 5σ statistical significance using 1D uncertainties, and at 3σ using the full covariance matrix, suggesting a physical difference between unobscured and obscured AGN, beyond the line-of-sight viewing angle. Furthermore, we find evidence for a halo mass dependence on reddening level within the Type I AGN population, which could support the existence of a dust-obscured phase. However, we also find that quite small systematic shifts in the redshift distributions of the AGN sample could explain current and previously observed differences in M

h
.
Supernova Shocks in Molecular Clouds: Shocks Driven into Dense Cores in IC 443 and 3C 391
Authors: Reach William T. , Tram Le Ngoc , DeWitt Curtis , Lesaffre Pierre , Godard Benjamin , Gusdorf Antoine 
Published: December 2024

Abstract
Supernova shocks into dense molecular cores in IC 443 (clumps B, C, and G) and 3C 391 were observed using the Stratospheric Observatory for Infrared Astronomy and complemented by archival data from the Herschel Space Observatory. The pure rotational transitions 0–0 S(1) and S(5) of H2, and the ground-state 110–101 transition of H2O, are all broadened, arising from molecules that survive the passage of the shock front. Theoretical models from the Paris–Durham shock code were analyzed to generate velocity profiles that approximately match the observations. The observations can be fit with two shock conditions, which approximate the range of densities in the preshock molecular cloud. The width and brightness of the S(5) lines require shocks into gas with a density of order 2000 cm−3, into which the IC 443 blast wave drives shocks with speed 60 km s−1. The brightness and narrower width of the S(1) lines requires different shocks, into gas with density of order 105 cm−3, with shock speeds of 10 km s−1. The H2O velocity distribution is also consistent with these shocks. The existence of shocks into dense gas shows that the bright shocked clumps in IC 443 were prestellar cores. It is unlikely that they will form stars soon after the passage of the shock front, given the input of kinetic and thermal energy from the shocks.