bluuebunny/crossref_metadata_2025_split huggingface dataset:
Geobacter
Strains Expressing Poorly Conductive Pili Reveal Constraints on Direct Interspecies Electron Transfer Mechanisms
Authors: Ueki Toshiyuki , Nevin Kelly P. , Rotaru Amelia-Elena , Wang Li-Ying , Ward Joy E. , Woodard Trevor L. , Lovley Derek R. 
Published: July 2018

ABSTRACT

Cytochrome-to-cytochrome electron transfer and electron transfer along conduits of multiple extracellular magnetite grains are often proposed as strategies for direct interspecies electron transfer (DIET) that do not require electrically conductive pili (e-pili). However, physical evidence for these proposed DIET mechanisms has been lacking. To investigate these possibilities further, we constructed
Geobacter metallireducens
strain Aro-5, in which the wild-type pilin gene was replaced with the
aro-5
pilin gene that was previously shown to yield poorly conductive pili in
Geobacter sulfurreducens
strain Aro-5.
G. metallireducens
strain Aro-5 did not reduce Fe(III) oxide and produced only low current densities, phenotypes consistent with expression of poorly conductive pili. Like
G. sulfurreducens
strain Aro-5,
G. metallireducens
strain Aro-5 displayed abundant outer surface cytochromes. Cocultures initiated with wild-type
G. metallireducens
as the electron-donating strain and
G. sulfurreducens
strain Aro-5 as the electron-accepting strain grew via DIET. However,
G. metallireducens
Aro-5/
G. sulfurreducens
wild-type cocultures did not. Cocultures initiated with the Aro-5 strains of both species grew only when amended with granular activated carbon (GAC), a conductive material known to be a conduit for DIET. Magnetite could not substitute for GAC. The inability of the two Aro-5 strains to adapt for DIET in the absence of GAC suggests that there are physical constraints on establishing DIET solely through cytochrome-to-cytochrome electron transfer or along chains of magnetite. The finding that DIET is possible with electron-accepting partners that lack highly conductive pili greatly expands the range of potential electron-accepting partners that might participate in DIET.

IMPORTANCE
DIET is thought to be an important mechanism for interspecies electron exchange in natural anaerobic soils and sediments in which methane is either produced or consumed, as well as in some photosynthetic mats and anaerobic digesters converting organic wastes to methane. Understanding the potential mechanisms for DIET will not only aid in modeling carbon and electron flow in these geochemically significant environments but will also be helpful for interpreting meta-omic data from as-yet-uncultured microbes in DIET-based communities and for designing strategies to promote DIET in anaerobic digesters. The results demonstrate the need to develop a better understanding of the diversity of types of e-pili in the microbial world to identify potential electron-donating partners for DIET. Novel methods for recovering as-yet-uncultivated microorganisms capable of DIET in culture will be needed to further evaluate whether DIET is possible without e-pili in the absence of conductive materials such as GAC.
Auricular Acupuncture for Treatment of Preoperative Anxiety in Patients Scheduled for Ambulatory Gynaecological Surgery: A Prospective Controlled Investigation with a Non-Randomised Arm
Authors: Wunsch Jakub K , Klausenitz Catharina , Janner Henriette , Hesse Thomas , Mustea Alexander , Hahnenkamp Klaus , Petersmann Astrid , Usichenko Taras I 
Published: July 2018

Objective Auricular acupuncture (AA) is a promising alternative treatment for situational anxiety. The aim of this pilot investigation was to test the acceptability and feasibility of AA as a treatment for preoperative anxiety (PA) in preparation for a subsequent randomised controlled trial. Methods AA was offered for treatment of PA to female patients who were scheduled for ambulatory gynaecological surgery. In patients who agreed, indwelling fixed needles were applied bilaterally at the points MA-IC1, MA-TF1, MA-SC, MA-AH7 and MA-T the day before surgery. Patients who declined AA but agreed to be examined constituted the control group (no intervention). State anxiety (primary outcome) was measured using the State-Trait-Anxiety Inventory (STAI) before AA (time I), the evening before surgery (time II) and immediately before surgery (time III). Anxiety was measured with a 100 mm visual analogue scale (VAS-100); heart rate, blood pressure and serum cortisol were also quantified. Results Data from 62 patients (32 with AA and 30 with no intervention) were analysed. Whereas preoperative anxiety was reduced after AA the evening before surgery (P<0.01), anxiety levels in the control group increased from the first to the last measurement (P<0.001). Secondary outcomes were comparable between the patients from both groups. Conclusions AA was acceptable and feasible as a treatment for preoperative anxiety. The results were used for the sample size calculation of a subsequent randomised controlled clinical trial. Trial Registration Number NCT02656966; Results.
Therapeutic Targeting of the Premetastatic Stage in Human Lung-to-Brain Metastasis
Authors: Singh Mohini , Venugopal Chitra , Tokar Tomas , McFarlane Nicole , Subapanditha Minomi K. , Qazi Maleeha , Bakhshinyan David , Vora Parvez , Murty Naresh K. , Jurisica Igor , Singh Sheila K. 
Published: July 2018

Abstract
Brain metastases (BM) result from the spread of primary tumors to the brain and are a leading cause of cancer mortality in adults. Secondary tissue colonization remains the main bottleneck in metastatic development, yet this “premetastatic” stage of the metastatic cascade, when primary tumor cells cross the blood–brain barrier and seed the brain before initiating a secondary tumor, remains poorly characterized. Current studies rely on specimens from fully developed macrometastases to identify therapeutic options in cancer treatment, overlooking the potentially more treatable “premetastatic” phase when colonizing cancer cells could be targeted before they initiate the secondary brain tumor. Here we use our established brain metastasis initiating cell (BMIC) models and gene expression analyses to characterize premetastasis in human lung-to-BM. Premetastatic BMIC engaged invasive and epithelial developmental mechanisms while simultaneously impeding proliferation and apoptosis. We identified the dopamine agonist apomorphine to be a potential premetastasis-targeting drug. In vivo treatment with apomorphine prevented BM formation, potentially by targeting premetastasis-associated genes KIF16B, SEPW1, and TESK2. Low expression of these genes was associated with poor survival of patients with lung adenocarcinoma. These results illuminate the cellular and molecular dynamics of premetastasis, which is subclinical and currently impossible to identify or interrogate in human patients with BM. These data present several novel therapeutic targets and associated pathways to prevent BM initiation.
Significance: These findings unveil molecular features of the premetastatic stage of lung-to-brain metastases and offer a potential therapeutic strategy to prevent brain metastases. Cancer Res; 78(17); 5124–34. ©2018 AACR.
Emerging Targeted Therapy for Tumors with NTRK Fusion Proteins
Authors: Kheder Ed S. , Hong David S. 
Published: July 2018

Abstract
The oncogenesis-promoting role of chromosomal rearrangements for several hematologic and solid malignancies is well recognized. However, identifying targetable, actionable, and druggable chromosomal rearrangements remains a challenge. Targeting gene fusions and chromosomal rearrangements is an effective strategy in treating gene rearrangement–driven tumors. The NTRK (Neurotrophic Tyrosine Receptor Kinase) gene family encodes three tropomyosin-related kinase (TRK) receptors that preserve central and peripheral nervous system development and function. NTRK genes, similar to other genes, are subject to alterations, including fusions. Preclinical studies have demonstrated that TRK fusion proteins promote oncogenesis by mediating constitutive cell proliferation and survival. Several clinical trials have estimated the safety and efficacy of TRK fusion kinase receptor inhibitors and have demonstrated encouraging antitumor activity in patients with NTRK-rearranged malignancies. Specifically, larotrectinib and entrectinib have emerged as potent, safe, and promising TRK inhibitors. Herein, we discuss the potential oncogenic characteristics of TRK fusion proteins in various malignancies and highlight ongoing clinical trials of kinase inhibitors targeting them.
Racial Differences in Long-Term Outcomes Among Older Survivors of In-Hospital Cardiac Arrest
Authors: Chen Lena M. , Nallamothu Brahmajee K. , Spertus John A. , Tang Yuanyuan , Chan Paul S. ,   the GWTG-R Investigators
Published: July 2018

Background:
Black patients have worse in-hospital survival than white patients after in-hospital cardiac arrest (IHCA), but less is known about long-term outcomes. We sought to assess among IHCA survivors whether there are additional racial differences in survival after hospital discharge and to explore potential reasons for differences.

Methods:
This was alongitudinal study of patients ≥65 years of age who had an IHCA and survived until hospital discharge between 2000 and 2011 from the national Get With The Guidelines–Resuscitation registry whose data could be linked to Medicare claims data. Sequential hierarchical modified Poisson regression models evaluated the proportion of racial differences explained by patient, hospital, and unmeasured factors. Our exposure was black or white race. Our outcome was survival at 1, 3, and 5 years.

Results:

Among 8764 patients who survived to discharge, 7652 (87.3%) were white and 1112 (12.7%) were black. Black patients with IHCA were younger, more frequently female, sicker with more comorbidities, less likely to have a shockable initial cardiac arrest rhythm, and less likely to be evaluated with coronary angiography after initial resuscitation. At discharge, black patients were also more likely to have at least moderate neurological disability and less likely to be discharged home. Compared with white patients and after adjustment only for hospital site, black patients had lower 1-year (43.6% versus 60.2%; relative risk [RR], 0.72), 3-year (31.6% versus 45.3%; RR, 0.71), and 5-year (23.5% versus 35.4%; RR, 0.67; all
P
<0.001) survival. Adjustment for patient factors explained 29% of racial differences in 1-year survival (RR, 0.80; 95% confidence interval, 0.75–0.86), and further adjustment for hospital treatment factors explained an additional 17% of racial differences (RR, 0.85; 95% confidence interval, 0.80–0.92). Approximately half of the racial difference in 1-year survival remained unexplained, and the degree to which patient and hospital factors explained racial differences in 3-year and 5-year survival was similar.

Conclusions:
Black survivors of IHCA have lower long-term survival compared with white patients, and about half of this difference is not explained by patient factors or treatments after IHCA. Further investigation is warranted to better understand to what degree unmeasured but modifiable factors such as postdischarge care account for unexplained disparities.
Association of Low Lysosomal Enzymes Activity With Brain Arterial Dilatation
Authors: Shah Harsh , Liong Christopher , Levy Oren A. , Waters Cheryl , Fahn Stanley , Marder Karen , Kang Un J. , Wolf Pavlina , Oliva Petra , Zhang Kate , Alcalay Roy N. , Gutierrez Jose 
Published: July 2018

Background and Purpose—
Absent or diminished α-galactosidase A (GLA) and acid α-glucosidase (GAA) enzyme activity are core features of Fabry and Pompe disease, respectively. Patients with Fabry or Pompe disease may have dilated intracranial arteries but whether lower GLA or GAA enzyme activity relates to brain arterial dilatation in other populations is unknown.

Methods—
Participants included Parkinson disease patients and nonblood-related controls, whose GLA and GAA enzymatic activities were measured in dried blood spots. Independent readers measured the axial arterial diameter of the ascending portion of the cavernous internal carotid arteries and the most proximal segment of the basilar artery in T2 black voids. Linear regression models were built to investigate the relationship between brain arterial diameters and lysosomal enzymatic activities.

Results—

The cohort included 107 participants (mean age, 66.5±10.3; 67% men). In an adjusted linear regression model, lower GLA activity was associated with larger brain arterial diameters (B=0.50±0.23,
P
=0.03). The strength of association was the greatest for the basilar artery diameter (B=0.80±0.33,
P
=0.02). Similarly, lower GAA activity was associated with an increased basilar arterial diameter (B=0.73±0.35,
P
=0.04).

Conclusions—
Lower GLA and GAA enzymatic activities were associated with larger brain arterial diameters, particularly the basilar artery diameter. Lower lysosomal enzymatic function in patients without Fabry or Pompe disease may play a role in brain arterial dilatation.
The History and Fulfilment of Western Rationality: Martin Jay’s The Eclipse of Reason
Authors: Doran Robert 
Published: July 2018

Abstract
This review-essay examines Martin Jay’s The Eclipse of Reason: On Late Critical Theory with a view toward understanding the stakes of its interpretative approach to intellectual history. I show how Jay's book aims to provide much more than a mere history of reason (although it does this admirably), for it seeks to legitimate and resuscitate an idea of reason in the face of more than one hundred years of anti-rationalist thought. I contend that Jay offers Habermas’s intersubjective rationality (“late Critical Theory”) as both an alternative to, but also in some sense a continuation of, the dominant forms of subjectivist and objectivist rationality Jay documents in his book. According to the logic of Jay’s account, then, Habermas represents in some sense the fulfillment of the history of Western rationality. My only critique of an otherwise excellent and fascinating study is that Jay’s Frankfurt School-centric narrative effectively inhibits a larger discussion of Habermas’s relevance to recent and current debates about the direction of philosophy. An elaboration specifically of the post-structuralist critique of reason would have greatly enhanced our understanding of the place of "late Critical Theory" in the intellectual landscape of our time.
On the taxonomy and natural history of the secretive Ischnocnema lactea Miranda-Ribeiro, 1923) (Anura: Brachycephaloidea: Brachycephalidae)
Authors: SILVA-SOARES THIAGO , MALAGOLI LEO RAMOS , CRUZ CARLOS ALBERTO GONÇALVES , CARAMASCHI ULISSES 
Published: July 2018

The original description of Ischnocnema lactea (Miranda-Ribeiro, 1923) was based on two specimens and an inaccurate type locality. These circumstances have obscured the identity of this name. Moreover, the I. lactea species has had a complex taxonomic history and, in general, specimens of species in this group are poorly represented in amphibian collections. The diagnosis of the series is confusing, with many non-exclusive and variable characters barely useful. An accurate diagnosis for the series requires a precise characterization of I. lactea, its type species. Recently discovered populations, museum data, and new information on the collector of the type specimen allowed the elucidation of important issues related to this species. Here we provide the redescription of Ischnocnema lactea based on the lectotype, and intraspecific variation based on newly collected specimens. Ischnocnema lactea is characterized by its large body size (adult 19.6–26.7 mm SVL; females 27.6–34.3 mm SVL); dorsum and flanks rugose, venter granular; vocal sac single and subgular, externally expanded; finger disks expanded, T-shaped, indented and truncate in fingers II–IV; toes relative length I<II<III<V<IV; black blotch on cloacal region, tarsus, and feet; moderate calcar tubercle present. Its advertisement call consists on a single short pulsed note ranging from 634 to 1060 milliseconds, with dominant frequency ranging from 2239.5 to 2756.2 Hz. Males call perched on vegetation mostly during daytime, starting early in the morning, and reproduction is explosive, associated to cold front rains. Ischnocnema lactea is a secretive and endemic inhabitant of the Serra do Mar in Southeastern Brazil. The type locality is redefined to the Municipality of Caraguatatuba.
Multilevel Tower Observations of Vertical Eddy Diffusivity and Mixing Length in the Tropical Cyclone Boundary Layer during Landfalls
Authors: Tang Jie , Zhang Jun A. , Aberson Sim D. , Marks Frank D. , Lei Xiaotu 
Published: July 2018

Abstract
This study analyzes the fast-response (20 Hz) wind data collected by a multilevel tower during the landfalls of Tropical Storm Lionrock (1006), Typhoon Fanapi (1011), and Typhoon Megi (1015) in 2010. Turbulent momentum fluxes are calculated using the standard eddy-correlation method. Vertical eddy diffusivity Km and mixing length are estimated using the directly measured momentum fluxes and mean-wind profiles. It is found that the momentum flux increases with wind speed at all four levels. The eddy diffusivity calculated using the direct-flux method is compared to that using a theoretical method in which the vertical eddy diffusivity is formulated as a linear function of the friction velocity and height. It is found that below ~60 m, Km can be approximately parameterized using this theoretical method, though this method overestimates Km for higher altitude, indicating that the surface-layer depth is close to 60 m in the tropical cyclones studied here. It is also found that Km at each level varies with wind direction during landfalls: Km estimated based on observations with landward fetch is significantly larger than that estimated using data with seaward fetch. This result suggests that different parameterizations of Km should be used in the boundary layer schemes of numerical models forecasting tropical cyclones over land versus over the ocean.
Statistically and Dynamically Downscaled, Calibrated, Probabilistic 10-m Wind Vector Forecasts Using Ensemble Model Output Statistics
Authors: Holman Bryan P. , Lazarus Steven M. , Splitt Michael E. 
Published: July 2018

Abstract
A computationally efficient method is developed that performs gridded postprocessing of ensemble 10-m wind vector forecasts. An expansive set of idealized WRF Model simulations are generated to provide physically consistent, high-resolution winds over a coastal domain characterized by an intricate land/water mask. The ensemble model output statistics (EMOS) technique is used to calibrate the ensemble wind vector forecasts at observation locations. The local EMOS predictive parameters (mean and variance) are then spread throughout the grid utilizing flow-dependent statistical relationships extracted from the downscaled WRF winds. In a yearlong study, the method is applied to 24-h wind forecasts from the Global Ensemble Forecast System (GEFS) at 28 east-central Florida stations. Compared to the raw GEFS, the approach improves both the deterministic and probabilistic forecast skill. Analysis of multivariate rank histograms indicates that the postprocessed forecasts are calibrated. A downscaling case study illustrates the method as applied to a quiescent easterly flow event. Strengths and weaknesses of the approach are presented and discussed.
Large-Eddy Simulation over Complex Terrain Using an Improved Immersed Boundary Method in the Weather Research and Forecasting Model
Authors: Bao Jingyi , Chow Fotini Katopodes , Lundquist Katherine A. 
Published: July 2018

Abstract
The Weather Research and Forecasting (WRF) Model is increasingly being used for higher-resolution atmospheric simulations over complex terrain. With increased resolution, resolved terrain slopes become steeper, and the native terrain-following coordinates used in WRF result in numerical errors and instability. The immersed boundary method (IBM) uses a nonconformal grid with the terrain surface represented through interpolated forcing terms. Lundquist et al.’s WRF-IBM implementation eliminates the limitations of WRF’s terrain-following coordinate and was previously validated with a no-slip boundary condition for urban simulations and idealized terrain. This paper describes the implementation of a log-law boundary condition into WRF-IBM to extend its applicability to general atmospheric complex terrain simulations. The implementation of the improved WRF-IBM boundary condition is validated for neutral flow over flat terrain and the complex terrain cases of Askervein Hill, Scotland, and Bolund Hill, Denmark. First, comparisons are made to similarity theory and standard WRF results for the flat terrain case. Then, simulations of flow over the moderately sloped Askervein Hill are used to demonstrate agreement between the IBM and terrain-following WRF results, as well as agreement with observations. Finally, Bolund Hill simulations show that WRF-IBM can handle steep topography (standard WRF fails) and compares well to observations. Overall, the new WRF-IBM boundary condition shows improved performance, though the leeside representation of the flow can be potentially further improved.
Do National Homicide Rates Follow Supranational Trends?
Authors: Rogers Meghan L. , Pridemore William Alex 
Published: July 2018

Objectives: We explored supranational trends in national homicide rates. We searched for a global trend, regional trends, and trends specific to other theoretically relevant groups of nations. We also tested two common metanarratives—modernization and conflict—as potential explanations for any global trend present in homicide rates. Method: We obtained annual homicide victimization rates for 94 nations between 1979 and 2013. We examined year-to-year differences, squared semipartial correlation coefficients to search for supranational trends, and pooled cross-sectional mixed models to test potential explanations of any global trend. Results: There was a very weak global homicide trend. We found strong regional trends in Eastern Europe and in Northern Europe, a weak trend for South and Central America, and no trend for Asia. Both wealthy and nonwealthy nations exhibited weak trends. Transitional nations shared a strong homicide trend. Modernization and conflict theories fared poorly as explanations for the weak global trend. Conclusions: The presence or absence of supranational homicide trends holds significant implications for theory. A weak global trend is evidence against widely held metanarratives such as the modernization, civilizing, and conflict perspectives. Strong subregional homicide trends in Eastern Europe and Northern Europe demand further exploration and should shift popular attention away from Western Europe. The lack of a homicide trend in developed or developing nations and the presence of a strong trend among transitional nations are curious features requiring further consideration.