bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering deterministic factors of predation pressures in deep time
Authors: Ishikawa Makiko , Kase Tomoki , Tsutsui Hidekazu 
Published: November 2018

AbstractPredation pressure occurs as a result of predation frequency and prey vulnerability. Although quantifying these factors individually is essential to precisely understand predation effects on evolution, they have been generally less accessible. Here, using a modified form of Poisson function, we quantified the frequencies and vulnerabilities, as well as the resulting predation pressures, concerning the shell drillers versus prey interactions from the Eocene and Miocene periods. Our analysis quantitatively revealed that low-spired shells tend to show increased vulnerability except for two planispiral species that exhibit an unexpectedly low vulnerability. We then identified septal structures within the two species that resemble those in nautiloids and ammonoids but which provided a defensive role against the predators, enhancing the mean lifetime by approximately 20%. The current approach enables us to quantitatively trace how predation frequency and prey vulnerability have interacted, been transformed spatio-temporally, and been a driving force of evolution at geological time scales.
Felis catus papillomavirus type-2 E6 binds to E6AP, promotes E6AP/p53 binding and enhances p53 proteasomal degradation
Authors: Altamura Gennaro , Power Karen , Martano Manuela , degli Uberti Barbara , Galiero Giorgio , De Luca Giovanna , Maiolino Paola , Borzacchiello Giuseppe 
Published: November 2018

AbstractE6 from high risk human papillomaviruses (HR HPVs) promotes ubiquitination and degradation of p53 tumour suppressor by mediating its binding to ubiquitin ligase E6AP in a ternary complex, contributing to cell transformation in cervical cancer. We have previously shown that Felis catus papillomavirus type −2 (FcaPV-2) E6 is expressed in feline squamous cell carcinoma (SCC) and displays the ability to bind p53 and decrease its protein levels in transfected CRFK cells. However, the mechanism of p53 downregulation has not yet been characterized. Here we show that FcaPV-2 E6 bound to E6AP, which in turn was bound by p53 exclusively in cells expressing the viral oncoprotein (CRFKE6). Furthermore, p53 was highly poly-ubiquitinated and underwent accumulation upon E6AP gene knockdown in CRFKE6. Half-life experiments and proteasome inhibition treatments indicated that down-regulation of p53 protein in CRFKE6 was due to accelerated proteasomal degradation. E6AP/p53 binding was also demonstrated in two feline SCC cell lines expressing FcaPV-2 E6, where p53 protein levels and poly-ubiquitination degree were proportional to E6 mRNA levels. The data obtained in both artificial and spontaneous in vitro models suggest that FcaPV-2 E6 degrades p53 through a molecular mechanism similar to HR HPVs, possibly contributing to the development of feline SCC.
Obesity and Type 2 Diabetes mellitus induce lipopolysaccharide tolerance in rat neutrophils
Authors: Kuwabara Wilson Mitsuo Tatagiba , Yokota Caroline Naomi Fukusawa , Curi Rui , Alba-Loureiro Tatiana Carolina 
Published: November 2018

AbstractObesity and diabetes implicate in various health complications and increased mortality caused by infection. Innate immune system is broadly affected by these diseases, leading the patients to an immunosuppressive state. A mechanism that leads innate immune cells to a less capacity of killing microorganism is the impaired TLR4 activation. TLR4 recognizes a component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS), and when activated increases the production of inflammatory substances. Neutrophils are components of the innate immune system and are the first responders to an invading agent. The correct activation of TLR4 in these cells is required for the initiation of the inflammatory process and elimination of the microorganisms. The aim of this study was to evaluate the influence of type 2 diabetes and obesity in the TLR4 pathway in rat neutrophils. Two experimental models were used: Goto-Kakizaki rats and high-fat-diet induced obese Wistar rats. To evaluate neutrophil response to LPS, intratracheal LPS instillation was used. Neutrophils from obese and diabetic animals exhibited tolerance to LPS, mainly by the impaired production of cytokines and chemokines and the low content of phospho-NFκB and phospho-IKBα. Neutrophils from both experimental models had increased cell death, impaired in vivo migration and myeloperoxidase activity.
Developmental pattern and structural factors of dendritic survival in cerebellar granule cells in vivo
Authors: Dhar Matasha , Hantman Adam W. , Nishiyama Hiroshi 
Published: November 2018

AbstractGranule cells (GCs) in the cerebellar cortex are important for sparse encoding of afferent sensorimotor information. Modeling studies show that GCs can perform their function most effectively when they have four dendrites. Indeed, mature GCs have four short dendrites on average, each terminating in a claw-like ending that receives both excitatory and inhibitory inputs. Immature GCs, however, have significantly more dendrites—all without claws. How these redundant dendrites are refined during development is largely unclear. Here, we used in vivo time-lapse imaging and immunohistochemistry to study developmental refinement of GC dendritic arbors and its relation to synapse formation. We found that while the formation of dendritic claws stabilized the dendrites, the selection of surviving dendrites was made before claw formation, and longer immature dendrites had a significantly higher chance of survival than shorter dendrites. Using immunohistochemistry, we show that glutamatergic and GABAergic synapses are transiently formed on immature GC dendrites, and the number of GABAergic, but not glutamatergic, synapses correlates with the length of immature dendrites. Together, these results suggest a potential role of transient GABAergic synapses on dendritic selection and show that preselected dendrites are stabilized by the formation of dendritic claws—the site of mature synapses.
The NnaR orphan response regulator is essential for the utilization of nitrate and nitrite as sole nitrogen sources in mycobacteria
Authors: Antczak Magdalena , Płocińska Renata , Płociński Przemysław , Rumijowska-Galewicz Anna , Żaczek Anna , Strapagiel Dominik , Dziadek Jarosław 
Published: November 2018

AbstractNitrogen is an essential component of biological molecules and an indispensable microelement required for the growth of cells. Nitrogen metabolism of Mycobacterium smegmatis is regulated by a number of transcription factors, with the glnR gene product playing a major role. Under nitrogen-depletion conditions, GlnR controls the expression of many genes involved in nitrogen assimilation, including the msmeg_0432 gene encoding NnaR, the homologue of a nitrite/nitrate transport regulator from Streptomyces coelicolor. In the present study, the role of NnaR in the nitrogen metabolism of M. smegmatis was evaluated. The ∆glnR and ∆nnaR mutant strains were generated and cultured under nitrogen-depletion conditions. Total RNA profiling was used to investigate the potential role of NnaR in the GlnR regulon under nitrogen-depletion and in nitrogen-rich media. We found that disruption of MSMEG_0432 affected the expression of genes involved in nitrite/nitrate uptake, and its removal rendered mycobacteria unable to assimilate nitrogen from those sources, leading to cell death. RNA-Seq results were validated using quantitative real-time polymerase chain reaction (qRT-PCR) and electrophoretic mobility shift assays (EMSAs). The ability of mutants to grow on various nitrogen sources was evaluated using the BIOLOG Phenotype screening platform and confirmed on minimal Sauton’s medium containing various sources of nitrogen. The ∆glnR mutant was not able to convert nitrates to nitrites. Interestingly, NnaR required active GlnR to prevent nitrogen starvation, and both proteins cooperated in the regulation of gene expression associated with nitrate/nitrite assimilation. The ∆nnaR mutant was able to convert nitrates to nitrites, but it could not assimilate the products of this conversion. Importantly, NnaR was the key regulator of the expression of the truncated haemoglobin trHbN, which is required to improve the survival of bacteria under nitrosative stress.
Midostaurin reduces Regulatory T cells markers in Acute Myeloid Leukemia
Authors: Gutierrez Lucas , Jang Miran , Zhang Tian , Akhtari Mojtaba , Alachkar Houda 
Published: November 2018

AbstractAcute myeloid leukemia (AML) is a heterogeneous hematological malignancy in which the only curative approach is allogeneic stem cell transplant (Allo-HSCT). The recognition and elimination of leukemic clones by donor T-cells contribute significantly to Allo-HSCT success. FLT3-ITD, a common mutation in AML, is associated with poor prognosis. Recently, midostaurin became the first FDA approved FLT3-inhibitor for pre-transplant patients with FLT3-ITD in combination with standard therapy. In addition to their multikinase activity which may affect T-cell signaling, FLT3-inhibitors induce apoptosis of malignant cells which may also enhance antigen presentation to activate T-cells. Considering the increased clinical use of these inhibitors in patients with AML, and the limited clinical benefit derived from their use as single agents, understanding how FLT3-inhibitors affect T cell population and function is needed to improve their clinical benefit. We examined the effect of four different FLT3 inhibitors (midostaurin, sorafenib, tandutinib, and quizartenib) on T cell populations in peripheral blood mononuclear cells (PBMC) obtained from healthy donors and from patients with AML. Midostaurin exhibited a significant decrease in CD4 + CD25 + FOXP3+ T cell population and FOXP3 mRNA expression in healthy and AML PBMCs. Similarly, samples collected from patients with AML treated with midostaurin showed a reduction in Tregs markers. Interferon-γ(IFN-γ), tumor necrosis factor-α(TNF-α), and IL-10 levels were also reduced following midostaurin treatment. Considering the FDA approval of midostaurin for use in patients with AML in the pre-transplant setting, our finding will have important clinical implication as it provides the rationale for functional investigation of the use of midostaurin in post-transplant patients.
Anomalous material-dependent transport of focused, laser-driven proton beams
Authors: Kim J. , McGuffey C. , Gautier D. C. , Link A. , Kemp G. E. , Giraldez E. M. , Wei M. S. , Stephens R. B. , Kerr S. , Poole P. L. , Madden R. , Qiao B. , Foord M. E. , Ping Y. , McLean H. S. , Fernández J. C. , Beg F. N. 
Published: November 2018

AbstractIntense lasers can accelerate protons in sufficient numbers and energy that the resulting beam can heat materials to exotic warm (10 s of eV temperature) states. Here we show with experimental data that a laser-driven proton beam focused onto a target heated it in a localized spot with size strongly dependent upon material and as small as 35 μm radius. Simulations indicate that cold stopping power values cannot model the intense proton beam transport in solid targets well enough to match the large differences observed. In the experiment a 74 J, 670 fs laser drove a focusing proton beam that transported through different thicknesses of solid Mylar, Al, Cu or Au, eventually heating a rear, thin, Au witness layer. The XUV emission seen from the rear of the Au indicated a clear dependence of proton beam transport upon atomic number, Z, of the transport layer: a larger and brighter emission spot was measured after proton transport through the lower Z foils even with equal mass density for supposed equivalent proton stopping range. Beam transport dynamics pertaining to the observed heated spot were investigated numerically with a particle-in-cell (PIC) code. In simulations protons moving through an Al transport layer result in higher Au temperature responsible for higher Au radiant emittance compared to a Cu transport case. The inferred finding that proton stopping varies with temperature in different materials, considerably changing the beam heating profile, can guide applications seeking to controllably heat targets with intense proton beams.
In-vivo probabilistic atlas of human thalamic nuclei based on diffusion- weighted magnetic resonance imaging
Authors: Najdenovska Elena , Alemán-Gómez Yasser , Battistella Giovanni , Descoteaux Maxime , Hagmann Patric , Jacquemont Sebastien , Maeder Philippe , Thiran Jean-Philippe , Fornari Eleonora , Bach Cuadra Meritxell 
Published: November 2018

AbstractThe thalamic nuclei are involved in many neurodegenerative diseases and therefore, their identification is of key importance in numerous clinical treatments. Automated segmentation of thalamic subparts is currently achieved by exploring diffusion-weighted magnetic resonance imaging (DW-MRI), but in absence of such data, atlas-based segmentation can be used as an alternative. Currently, there is a limited number of available digital atlases of the thalamus. Moreover, all atlases are created using a few subjects only, thus are prone to errors due to the inter-subject variability of the thalamic morphology. In this work, we present a probabilistic atlas of anatomical subparts of the thalamus built upon a relatively large dataset where the individual thalamic parcellation was done by employing a recently proposed automatic diffusion-based clustering method. Our analyses, comparing the segmentation performance between the atlas-based and the clustering method, demonstrate the ability of the provided atlas to substitute the automated diffusion-based subdivision in the individual space when the DW-MRI is not available.
School-Aged Children's Phonological Accuracy in Multisyllabic Words on a Whole-Word Metric
Authors: Mason Glenda K. 
Published: November 2018

Purpose
The purpose of this study is to examine differences in phonological accuracy in multisyllabic words (MSWs) on a whole-word metric, longitudinally and cross-sectionally, for elementary school–aged children with typical development (TD) and with history of protracted phonological development (PPD).

Method

Three mismatch subtotals, Lexical influence, Word Structure, and segmental Features (forming a Whole Word total), were evaluated in 3 multivariate analyses: (a) a longitudinal comparison (
n
= 22), at age 5 and 8 years; (b) a cross-sectional comparison of 8- to 10-year-olds (
n
= 12 per group) with TD and with history of PPD; and (c) a comparison of the group with history of PPD (
n
= 12) with a larger 5-year-old group (
n
= 62).

Results

Significant
effect sizes
(η

p

2
) found for mismatch totals were as follows: (a) moderate (Lexical, Structure) and large (Features) between ages 5 and 8 to 10 years, mismatch frequency decreasing developmentally, and (b) large between 8- to 10-year-olds with TD and with history of PPD (Structure, Features; minimal lexical influences), in favor of participants with TD. Mismatch frequencies were equivalent for 8- to 10-year-olds with history of PPD and 5-year-olds with TD. Classification accuracy in original subgroupings was 100% and 91% for 8- to 10-year-olds with TD and with history of PPD, respectively, and 86% for 5-year-olds with TD.

Conclusion
Phonological accuracy in MSW production was differentiated for elementary school–aged children with TD and PPD, using a whole-word metric. To assist with the identification of children with ongoing PPD, the metric has the ability to detect weaknesses and track progress in global MSW phonological production.
Symmetric dual gate insulator‐based FinFET module and design window for reliable circuits
Authors: Yadav Nandakishor , Shah Ambika Prasad , Beohar Ankur , Vishvakarma Santosh Kumar 
Published: November 2018

High‐k spacer and gate insulator materials have been exhaustively studied nowadays for the enhancement of electrostatic control and reduction of short‐channel effects in scaled devices. The work presents a high‐performance and charge trap tolerant FinFET module at 10 nm gate length. Dual layer gate insulator (inner low‐k and outer high‐k) introduces to reduce charge trapping from the channel and outside into the gate oxide. It reduces the gate leakage current by 51.6% compared to conventional FinFET. Further, they demonstrate single charge trapping (SCT) induce effects and proposed optimised high‐k spacer width of the SCT tolerant design. SCT analysis is presented in different high‐k spacer materials and back‐gate voltages. Process variation sources such as line edge roughness and line width roughness are also analysed for the circuit design.
(3200) Phaethon: Bulk density from Yarkovsky drift detection
Authors: Hanuš J. , Vokrouhlický D. , Delbo’ M. , Farnocchia D. , Polishook D. , Pravec P. , Hornoch K. , Kučáková H. , Kušnirák P. , Stephens R. , Warner B. 
Published: November 2018

Context.The recent close approach of the near-Earth asteroid (3200) Phaethon offered a rare opportunity to obtain high-quality observational data of various types.Aims.We used the newly obtained optical light curves to improve the spin and shape model of Phaethon and to determine its surface physical properties derived by thermophysical modeling. We also used the available astrometric observations of Phaethon, including those obtained by the Arecibo radar and theGaiaspacecraft, to constrain the secular drift of the orbital semimajor axis. This constraint allowed us to estimate the bulk density by assuming that the drift is dominated by the Yarkovsky effect.Methods.We used the convex inversion model to derive the spin orientation and 3D shape model of Phaethon, and a detailed numerical approach for an accurate analysis of the Yarkovsky effect.Results.We obtained a unique solution for Phaethon’s pole orientation at (318 ° , − 47 ° ) ecliptic longitude and latitude (both with an uncertainty of 5°), and confirm the previously reported thermophysical properties (D = 5.1 ± 0.2 km, Γ = 600 ± 200J m−2 s−0.5 K−1). Phaethon has a top-like shape with possible north-south asymmetry. The characteristic size of the regolith grains is 1 − 2 cm. The orbit analysis reveals a secular drift of the semimajor axis of −(6.9 ± 1.9)×10−4au Myr−1. With the derived volume-equivalent size of 5.1 km, the bulk density is 1.67 ± 0.47 g cm−3. If the size is slightly larger ∼5.7 − 5.8 km, as suggested by radar data, the bulk density would decrease to 1.48 ± 0.42 g cm−3. We further investigated the suggestion that Phaethon may be in a cluster with asteroids (155140) 2005 UD and (225416) 1999 YC that was formed by rotational fission of a critically spinning parent body.Conclusions.Phaethon’s bulk density is consistent with typical values for large (> 100 km) C-complex asteroids and supports its association with asteroid (2) Pallas, as first suggested by dynamical modeling. These findings render a cometary origin unlikely for Phaethon.
CFD modelling and validation of the rotary lobe compressed air expander
Authors: Kurkus-Gruszecka Michalina , Krawczyk Piotr , Łapka Piotr 
Published: November 2018

The article covers the CFD analysis of the compressed air rotary lobe expander. Compressed air engines are commonly used in the explosion hazard zones, where the others power sources cannot be applied. The lobe construction seems to be cheaper solution in comparison with the classic turbine expanders at the corresponding power level.
In the paper the rotary lobe compressed air expander model was developed in the ANSYS CFX software. During implementation of the proposed numerical model the mesh motion problem was solved, which was challenging due to relatively small gaps between lobes and the housing as well as due to high rotary speed of lobes. What is more, the compressible fluid model was applied. Therefore, these conditions make the undertaken problem more complex and calculation more time consuming. Finally, the model was validated applying the catalogue data of the producer.
In the paper description of the developed model was presented. Then the results of simulations with their validation were shown which proved the correctness and accuracy of the model. The developed tool might be very useful for the further analysis of the expander including its optimization in terms of improvement of its efficiency or energy gain.
Role of Cross-training in Orthopaedic Injuries and Healthcare Burden in Masters Swimmers
Authors: Baker Brett , Lapierre Stephanie , Tanaka Hirofumi 
Published: November 2018

AbstractWe determined whether the incidence of injuries would increase with advancing age and whether the participation in cross-training would be related to a decreased rate of injuries and healthcare costs in Masters swimmers. A total of 499 swimmers (55±14 years of age) belonging to US Masters Swimming completed a comprehensive questionnaire that included questions regarding their medical history as well as their training history. In average, swimmers had been training for 13±12 years, and 35, 40, and 47% participated in running, cycling, and dryland resistance training, respectively. According to the logistic regression, prevalence of injuries increased significantly but modestly with advancing age (p<0.05). Linear regression analysis showed that for every 1 month increase in the length of injury, healthcare costs increased by 7.4% (p<0.05). Linear regression and logistic regression analyses determined that overall volume of swimming training was not related to age or incidence of injuries, respectively. Multinomial logistic regression analyses using age and sex as predictors demonstrated that the odds of reporting a swim-related injury were significantly lower for individuals who participated in any number of cross-training activities compared with those who do not cross-train (p<0.05). Regular participation in any cross-training modality was inversely related to age (p<0.05). We concluded that the diversification of the overall training program by incorporating cross-training may be an important strategy to reduce sport-specific injuries among Masters athletes.