bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair
Authors: Mu Chunlan , Li Xiaoyong , Yang Jiamei , Tian Geng G. , Bai Hepeng , Lin Wenhui , Wang Linhui , Wu Ji 
Published: December 2024

AbstractOvarian dysfunction is a major factor leading to female infertility. Understanding how to improve or reshape ovarian function has become an important entry point for preventing and treating female infertility caused by ovarian dysfunction. Here, plant‐derived compounds are screened for in vitro activity upon ovarian organoids derived from feeder‐free female germline stem cells. Tetrahydroxy stilbene glucoside (TSG) is found to promote the development of ovarian organoids. Single nucleus transcriptome sequencing and spatial transcriptome sequencing are used to establish a comprehensive spatiotemporal map to elucidate the role of TSG in ovarian organoid development, encompassing cell types and subtypes, transcription factors, pseudo‐time sequence, and cell communication dynamics. This analysis indicates that TSG promotes ovarian organoid development through the vascular endothelial growth factor A‐Eph receptor B2 ligand‐receptor pair between granulosa cells and oocytes. This study has enhanced the understanding of the mechanisms of ovarian organoid development, establishes a technical platform for screening compounds for treating infertility and related diseases, and lays a foundation for clinically applying plant‐derived compounds.
Effects of Alkyl Spacer Length in Carbazole‐Based Self‐Assembled Monolayer Materials on Molecular Conformation and Organic Solar Cell Performance
Authors: Chen Qiaonan , Sun Kangbo , Franco Leandro R. , Wu Jingnan , Öhrström Lars , Liu Xianjie , Gumbo Maureen , Ozório Mailde S. , Araujo C. Moyses , Zhang Guangye , Johansson André , Moons Ellen , Fahlman Mats , Yu Donghong , Wang Yufei , Wang Ergang 
Published: December 2024

AbstractCarbazole‐based self‐assembled monolayer (SAM) materials as hole transport layers (HTL) have led organic solar cells (OSCs) to state‐of‐the‐art photovoltaic performance. Nonetheless, the impact of the alkyl spacer length of SAMs remains inadequately understood. To improve the knowledge, four dichloride‐substituted carbazole‐based SAMs (from 2Cl‐2PACz to 2Cl‐5PACz) with spacer lengths of 2–5 carbon atoms is developed. Single crystal analyses reveal that SAMs with shorter spacers exhibit stronger intermolecular interactions and denser packing. The molecular conformation of SAMs significantly impacts their molecular footprint and coverage on ITO. These factors result in the highest coverage of 2Cl‐2PACz and the lowest coverage for 2Cl‐3PACz on ITO. OSCs based on PM6:L8‐BO with 2Cl‐2PACz as HTL achieved high efficiencies of 18.95% and 18.62% with and without methanol rinsing of the ITO/SAMs anodes, corresponding to monolayer and multilayer structures, respectively. In contrast, OSCs utilizing the other SAMs showed decreased efficiencies as spacer length increased. The superior performance of 2Cl‐2PACz can be attributed to its shorter spacer, which reduces series resistance, hole tunneling distance, and barrier. This work provides valuable insights into the design of SAMs for high‐performance OSCs.
Nerve‐Inspired Optical Waveguide Stretchable Sensor Fusing Wireless Transmission and AI Enabling Smart Tele‐Healthcare
Authors: Li Tianliang , Wang Qian'ao , Cao Zichun , Zhu Jianglin , Wang Nian , Li Run , Meng Wei , Liu Quan , Yu Shifan , Liao Xinqin , Song Aiguo , Tan Yuegang , Zhou Zude 
Published: December 2024

AbstractFlexible strain monitoring of hand and joint muscle movement is recognized as an effective method for the diagnosis and rehabilitation of neurological diseases such as stroke and Parkinson's disease. However, balancing high sensitivity and large strain, improving wearing comfort, and solving the separation of diagnosis and treatment are important challenges for further building tele‐healthcare systems. Herein, a hydrogel‐based optical waveguide stretchable (HOWS) sensor is proposed in this paper. A double network structure is adopted to allow the HOWS sensor to exhibit high stretchability of the tensile strain up to 600% and sensitivity of 0.685 mV %−1. Additionally, the flexible smart bionic fabric embedding the HOWS sensor, produced through the warp and weft knitting, significantly enhances wearing comfort. A small circuit board is prepared to enable wireless signal transmission of the designed sensor, thereby improving the daily portability. A speech recognition and human‐machine interaction system, based on sensor signal acquisition, is constructed, and the convolutional neural network algorithm is integrated for disease assessment. By integrating sensing, wireless transmission, and artificial intelligence (AI), a smart tele‐healthcare system based on HOWS sensors is demonstrated to hold great potential for early warning and rehabilitation monitoring of neurological diseases.
Divergent Adsorption Regulation in Metal–Organic Frameworks for Highly Efficient CF4/C2F6 Separation
Authors: Xu Guihong , Ke Tian , Fan Rongrong , Tan Kaiyuan , Zhang Wenjun , Su Baogen , Zhang Zhiguo , Bao Zongbi , Ren Qilong , Yang Qiwei 
Published: December 2024

AbstractThe efficient removal of low‐concentration components from homologous mixtures is often hampered by the co‐directional effect of traditional thermodynamic regulation approaches, typically leading to a trade‐off between adsorption capacity and selectivity. Focusing this challenge on the critical task of purifying perfluorocarbons in electronics industry, a divergent regulation strategy is reported that significantly improves the separation efficiency of low‐concentration hexafluoroethane (C2F6) from tetrafluoromethane (CF4). This approach involves the selective shielding of open metal sites and the modulation of channel geometry within an electron‐deficient ligand‐based pore environment, thereby facilitating a C2F6 dense‐packing accommodation mode while weakening the CF4 affinity due to the reduced host‐guest interactions. Simultaneously enhanced C2F6 adsorption and reduced CF4 adsorption are achieved, resulting in record‐high low‐pressure C2F6 uptake and C2F6/CF4 selectivity. Comprehensive insights into the unique separation mechanism are illustrated through a combination of solid‐state MAS nuclear magnetic resonance (SSNMR), molecular simulations, and meticulously designed comparative experiments. As a result, benchmark C2F6/CF4 separation performance is achieved, as demonstrated by the unprecedented electronic‐grade (over 99.999%) CF4 productivity (401 L kg−1) obtained from an industrially relevant C2F6/CF4 (3:97) mixture, as well as the excellent water/air/heat stability and recyclability.
Single‐Cell Simultaneous Metabolome and Transcriptome Profiling Revealing Metabolite‐Gene Correlation Network
Authors: Mao Xiying , Xia Dandan , Xu Miao , Gao Yan , Tong Le , Lu Chen , Li Weiqi , Xie Runmin , Liu Qinghuai , Jiang Dechen , Yuan Songtao 
Published: December 2024

AbstractMetabolic studies at the single cell level can directly define the cellular phenotype closest to physiological or disease states. However, the current single cell metabolome (SCM) study using mass spectroscopy has difficulty giving a complete view of the metabolic activity in the cell, and the prediction of the metabolism‐phenotype relationship is limited by the potential inconsistency between transcriptomic and metabolic levels. Here, the single‐cell simultaneous metabolome and transcriptome profiling method (scMeT‐seq) is developed at one single cell, based on sub‐picoliter sampling from the cell for the initial metabolome profiling followed by single cell transcriptome sequencing. This design not only provides sufficient cytoplasm for SCM but also nicely keeps the cellular viability for the accurate transcriptomic analysis in the same cell. Integrative analysis of scMeT‐seq reveals both dynamical and cell state‐specific associations between metabolome and transcriptome in the macrophages with defined metabolic perturbations. Moreover, metabolite signatures are mapped to the single‐cell trajectory and gene correlation network of macrophage transition, which allows the unsupervised functional interpretation of metabolome. Thus, the established scMeT‐seq should lead to a new perspective in metabolic research by transforming metabolomics from a metabolite snapshot to a functional approach.
Injectable Hierarchical Bioactive Hydrogels with Fibroblast Growth Factor 21/Edaravone/Caffeic Acid Asynchronous Delivery for Treating Parkinson's Disease
Authors: Xu Junpeng , Dai Peng , Zhang Chen , Dong Na , Li Caiyan , Tang Chonghui , Jin Zhihao , Lin Shih‐Ho , Ye Luyang , Sun Tianmiao , Jin Yukai , Wu Fenzan , Luo Lihua , Wu Ping , Li Shengcun , Li Xiaokun , Hsu Shan‐hui , Jiang Dawei , Wang Zhouguang 
Published: December 2024

AbstractParkinson's disease (PD) is one of the most common long‐term neurodegenerative disorders, with multiple comorbid psychiatric and behavioral abnormalities. The combination of clinical drugs targeting different symptoms with smart hydrogels to achieve asynchronous releases is highly translational and challenging. Here, a hierarchical bioactive hydrogel (OACDP) is designed with asynchronous release based on PD pathology. The hydrogel with caffeic acid‐grafted polymer main chain is crosslinked using a micellar nanocrosslinker, with sufficient modulus (≈167 Pa), antioxidant activity (> 50%), injectability (30‐gauge syringe needle), and shape‐adaptability. Each of the three drugs (caffeic acid, fibroblast growth factor 21, and Edaravone) is separately engaged in different micro‐ or nanostructures of the hydrogel and released with asynchronous kinetics of first‐order release, zero‐order release, or matching Korsmeyer–Peppas model. The triple‐loaded hydrogel is injected into the brains of PD rats, showing behavioral improvement. Histological analysis revealed that the triple‐loaded OACDP hydrogels are effective in achieving immediate neuroprotection, i.e., reduction the loss of tyrosine hydroxylase in substantia nigra compacta and striatum (retained ≈10‐fold versus control), decreasing oxidative stress, reducing astrocyte and microglia activation, and stimulating the AMPK/PGC‐1α axis to regulate the mitochondrial function, providing a multi‐dimensional PD therapy. The asynchronous release of OACDP hydrogel provides a new conception for PD treatment and other neurodegenerative diseases.
Selective Plasmonic C─H Bond Editing for Low‐Temperature Light‐Driven Greenhouse Gas Upgrading
Authors: Sun Nan , Liu Xianglei , Tian Cheng , Xu Qiao , Xuan Yimin 
Published: December 2024

AbstractLight‐driven greenhouse gases upgrading (GGU) into syngas is a promising approach to reduce CO2 emissions and supply green fuels simultaneously. However, this reaction usually suffers from high operation temperature and low conversion rate due to stringent thermodynamic constraints. Herein, a selective plasmonic CH bond editing strategy is presented via incorporating ultralow amounts of Cu into Ni‐based catalysts by electrostatic adsorption. A remarkable CO2 conversion rate 2.69 times as high as the thermodynamic limit and extraordinary light‐to‐fuel efficiency of 24.95% at low temperature of 500 °C are achieved, outperforming the state‐of‐the‐art literature reports. The extremely low fraction of Cu (0.06 wt%) assists the injection of localized surface plasmon resonance induced hot electrons into the antibonding orbital of reactants, accelerating cleavage of the first CH bond of *CH4, which is usually the rate‐determining step for GGU. Simultaneously, *CH intermediates are induced to proceed along *CH+*O = *CHO rather than *CH = *C+*H, thus avoid complete cleavage of CH4 and subsequent coke deposition, leading to stable on‐stream operation over 20 h. Such a selective CH bond editing approach enables ordered conversion of CH4 and CO2 with high conversion rate and high efficiency synergistically beyond thermodynamic limits.
Selective Synthesis of Ethane from Methane by a Photocatalytic Chemical Cycle Process
Authors: Yang Jianlong , Xiong Lunqiao , Wang Chao , Luo Lei , Jing Liqiang , Martsinovich Natalia , Tang Junwang 
Published: December 2024

AbstractSynthesis of value‐added chemicals from methane remains a great challenge due to its high energy requirement, low conversion efficiency, and unavoidable over‐oxidation of desired products. Here, the integration of a photon‐driven chemical cycle process with a continuous flow reactor over the Co0.2Pd1.8‐TiO2 catalyst has led to the continuous synthesis of C2H6 from CH4 with ≈100% selectivity under ambient conditions, simultaneously avoiding mixing flammable gas methane with O2 for the chemicals production. Such high selectivity and activity are due to the active lattice oxygen of PdOL and the oxygen‐lean condition characterized in the chemical cycle, together with Co single atoms for the regeneration of the photocatalyst surface during the chemical cycle process. The consumed oxygen in PdOL can be compensated by air during the subsequent catalyst regeneration process, leading to the stable activity during a 43 cycles test. Furthermore, this work to some extent demonstrates that the chemical cycle process not only improves the technoeconomic viability but also enhances safety of the process.
Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodents
Authors: Zhang Hao , Gao Dongmei , Hu Minghu , Zhou Wanqing , Han Muxuan , Sun Ya , Zhang Yang , Wang Jieqiong , Gao Mingzhou 
Published: December 2024

AbstractBackgroundPreviously, a chronic social defeat stress (CSDS) model has been widely‐adopted for assessing depressive‐like behaviors in animals. However, there is still room for improvement in the CSDS model to safeguard study accuracy and the welfare of lab rodents. Our study team developed a novel, standardized apparatus to induce CSDS in rodents and assessed the model's practical adaptability.MethodsAn innovative CSDS cage apparatus and water bottle was designed. To evaluate the effectiveness of the newly developed tools, a variety of animal models, including the tail suspension test (TST), sucrose preference test, forced swimming test (FST), novelty‐suppressed feeding test, female urine sniffing test, and open field test (OFT), were adopted to assess depressive‐like behaviors in mice. Fluoxetine treatment was also administered to observe the reversal effect, as part of the validation.ResultsThe CSDS cage apparatus resulted in the manifestation of depressive‐like behaviors in the model mice. Significant reductions in sucrose preference and urine sniffing time were observed, while the OFT revealed decreased central zone total distance, residence time, and frequency of entry. Moreover, increased immobility was found in the FST and TST. Fluoxetine treatment was found to successfully reverse the modeling effect.ConclusionThe CSDS cage apparatus was validated for enhanced usability and addressed the previous challenges of water bottle leakage and lab rodent welfare issues. The consistent results from multiple behavioral tests also supported real‐world application of the apparatus, offering researchers a promising alternative to conventional rodent cages.
Improving dexamethasone drug loading and efficacy in treating rheumatoid arthritis via liposome: Focusing on inflammation and molecular mechanisms
Authors: Zamanian Mohammad Yasin , Zafari Hamidreza , Osminina Maria K. , Skakodub Alla A. , Al‐Aouadi Raed Fanoukh Aboqader , Golmohammadi Maryam , Nikbakht Nikta , Fatemi Iman 
Published: December 2024

AbstractRheumatoid arthritis (RA) is a chronic autoimmune disease that affects approximately 0.46% of the global population. Conventional therapeutics for RA, including disease‐modifying antirheumatic drugs (DMARDs), nonsteroidal anti‐inflammatory drugs (NSAIDs), and corticosteroids, frequently result in unintended adverse effects. Dexamethasone (DEX) is a potent glucocorticoid used to treat RA due to its anti‐inflammatory and immunosuppressive properties. Liposomal delivery of DEX, particularly when liposomes are surface‐modified with targeting ligands like peptides or sialic acid, can improve drug efficacy by enhancing its distribution to inflamed joints and minimizing toxicity. This study investigates the potential of liposomal drug delivery systems to enhance the efficacy and targeting of DEX in the treatment of RA. Results from various studies demonstrate that liposomal DEX significantly inhibits arthritis progression in animal models, reduces joint inflammation and damage, and alleviates cartilage destruction compared to free DEX. The liposomal formulation also shows better hemocompatibility, fewer adverse effects on body weight and immune organ index, and a longer circulation time with higher bioavailability. The anti‐inflammatory mechanism is associated with the downregulation of pro‐inflammatory cytokines like tumor necrosis factor‐α (TNF‐α) and B‐cell–activating factor (BAFF), which are key players in the pathogenesis of RA. Additionally, liposomal DEX can induce the expression of anti‐inflammatory cytokines like interleukin‐10 (IL‐10), which has significant anti‐inflammatory and immunoregulatory properties. The findings suggest that liposomal DEX represents a promising candidate for effective and safe RA therapy, with the potential to improve the management of this debilitating disease by providing targeted delivery and sustained release of the drug.
Strong Magneto‐Chiroptical Effects through Introducing Chiral Transition‐Metal Complex Cations to Lead Halide
Authors: Lu Haolin , Qi Fenglian , Wang Hebin , He Tengfei , Sun Bing , Gao Xiaoqing , Comstock Andrew H. , Gull Sehrish , Zhang Yunxin , Qiao Tianjiao , Shao Tianyin , Zheng You‐Xuan , Sun Dali , Chen Yongsheng , Zhang Hao‐Li , Tang Zhiyong , Long Guankui 
Published: December 2024

AbstractThe interplay between chirality with magnetism can break both the space and time inversion symmetry and have wide applications in information storage, photodetectors, multiferroics and spintronics. Herein, we report the chiral transition‐metal complex cation‐based lead halide, R‐CDPB and S‐CDPB. In contrast with the traditional chiral metal halides with organic cations, a novel strategy for chirality transfer from the transition‐metal complex cation to the lead halide framework is developed. The chiral complex cations directly participate the band structure and introduce the d‐d transitions and tunable magneto‐chiroptical effects in both the ultraviolet and full visible range into R‐CDPB and S‐CDPB. Most importantly, the coupling between magnetic moment of the complex cation and chiroptical properties is confirmed by the magneto‐chiral dichroism. For the band‐edge transition, the unprecedented modulation of +514 % for S‐CDPB and −474 % for R‐CDPB was achieved at −1.3 Tesla. Our findings demonstrate a novel strategy to combine chirality with magnetic moment, and provide a versatile material platform towards magneto‐chiroptical and chiro‐spintronic applications.
Ferrocene‐Functionalized Atomically Precise Metal Clusters Exhibit Synergistically Enhanced Performance for CO2 Electroreduction
Authors: Deng Guocheng , Yun Hyewon , Chen Yuping , Yoo Seungwoo , Lee Kangjae , Jang Junghwan , Liu Xiaolin , Lee Chan Woo , Tang Qing , Bootharaju Megalamane S. , Hwang Yun Jeong , Hyeon Taeghwan 
Published: December 2024

AbstractThe integration of organometallic compounds with metal nanoparticles can, in principle, generate hybrid nanocatalysts endowed with augmented functionality, presenting substantial promise for catalytic applications. Herein, we synthesize an atomically precise metal cluster (Ag9Cu6) catalyst integrated with alkynylferrocene molecules (Ag9Cu6‐Fc). This hybrid catalyst design facilitates a continuous electron transfer channel via an ethynyl bridge and establishes a distinctive local chemical environment, resulting in remarkably enhanced catalytic activity in CO2 electroreduction. The Ag9Cu6‐Fc catalyst achieves a record‐high product selectivity of CO Faradaic efficiency of 100 % and an industrial‐level CO partial current density of −680 mA/cm2, surpassing the performance of the Ag9Cu6 cluster (62 % and −230 mA/cm2, respectively) without ferrocene functionalization in a membrane electrode assembly cell. Operando experimental and computational findings offer valuable insights into the role of ferrocene functionalization in synergistically improving the catalytic performance of metal clusters, propelling the advancement of metallic‐organometallic hybrid nanoparticles for energy conversion technologies.
Expedited SARS‐CoV‐2 Main Protease Inhibitor Discovery through Modular ‘Direct‐to‐Biology’ Screening
Authors: Wilders Harry , Biggs George , Rowe Sam M. , Cawood Emma E. , Riziotis Ioannis G. , Rendina Alan R. , Grant Emma K. , Pettinger Jonathan , Fallon David J. , Skehel Mark , House David , Tomkinson Nicholas C. O. , Bush Jacob T. 
Published: December 2024

AbstractReactive fragment (RF) screening has emerged as an efficient method for ligand discovery across the proteome, irrespective of a target's perceived tractability. To date, however, the efficiency of subsequent optimisation campaigns has largely been low‐throughput, constrained by the need for synthesis and purification of target compounds. We report an efficient platform for ‘direct‐to‐biology’ (D2B) screening of cysteine‐targeting chloroacetamide RFs, wherein synthesis is performed in 384‐well plates allowing direct assessment in downstream biological assays without purification. Here, the developed platform was used to optimise inhibitors of SARS‐CoV‐2 main protease (MPro), an established drug target for the treatment of COVID‐19. An initial RF hit was developed into a series of potent inhibitors, and further exploration using D2B screening enabled a ‘switch’ to a reversible inhibitor series. This example of ligand discovery for MPro illustrates the acceleration that D2B chemistry can offer for optimising RFs towards covalent inhibitor candidates, as well as providing future impetus to explore the evolution of RFs into non‐covalent ligands.
Induced Charge‐Compensation Effect for Boosting Photocatalytic Water Splitting in Covalent Organic Frameworks
Authors: Zou Lei , Si Duanhui , Yang Shuailong , Chen Ziao , Huang Yuanbiao , Cao Rong 
Published: December 2024

AbstractImine‐based covalent organic frameworks (COFs) are promising for photocatalytic water splitting, but their performance is often constrained by inefficient charge separation due to the high electron localization nature of polar imine bonds. In this study, we have optimized the electron delocalization across the imine linkage within a COF by implementing a charge compensation effect. This effect is achieved when a strong electron‐donating thieno[3,2‐b]thiophene linker is directly attached to the iminic carbon of a zinc‐porphyrinic COF. This modification significantly reduces the electron binding effect within the imine bonds of the COF, facilitating both in‐plane charge separation and out‐plane charge transfer to the catalytic site. Conversely, the use of strong electron‐withdrawing pyrizine linker aggravates the electron localization at the imine linkage in the ZnP−Pz variant. Consequently, ZnP−Tt shows a substantially improved photocatalytic water‐splitting activity under visible light irradiation, with a hydrogen evolution of 44288±2280 μmol g−1 in 4 h, which exceeds the ZnP−Pz counterpart by a factor of 10. These results offer fresh perspectives for the design of imine‐based COFs to overcome their limitations in charge separation efficiency.