bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acanthocephalan in Xenochrophis piscator Snake in Sidoarjo Indonesia
Authors: Audini Inggarsetya Syah , Suwanti Lucia Tri , Koesdarto Setiawan , Poetranto Emmanuel Djoko 
Published: March 2018

Xenochrophis piscator is a snake that often found in Indonesia, particularly in Java Island. Xenochrophis piscator is a non-venomous snake and often used for food, traditional medicine and as pets in Indonesia. Snakes can be infected by different types of parasites which are zoonotic. One of the zoonotic helminth infect snakes is acanthocephalan. Acanthocephalan can be transmitted to humans by ingesting snake products. We investigate the incidence of helminthiasis in X. piscator from snakes collector in Tulangan district, Sidoarjo, East Java. Parasites were collected from X. piscator organs. Identification of parasites was in carmine stain using light microscope for examination. Sixty snakes were observed and sixteen snakes were positively infected by adult acanthocephalan (26.67%). Acanthocephalan was found in mesenterium and fascia of X. piscator. Keyword : Xenochrophis piscator, acanthocephalan, helminthiasis, zoonosis.
The Identification Blood Parasites On Pig (Susdomesticus) In Polewali Mandar District
Authors: Arfin Silvana , Muslimin Lucia , Ris Adryani 
Published: March 2018

Parasite is a detrimental living creature that using other living creature food in its life. Blood parasite is one of the quite important livestock disease which is endemic and can lead to fairly large economic losses which include weight loss, employment loss, and death of livestock. Blood parasites that usually attack livestock are Theileria sp, Babesia sp, Anaplasma and Plasmodium. The purpose of the research is to identify blood parasite of swine in Polewali Mandar district. This research was conducted on 25 August to 23 September 2016. The samples are 80 swine which obtained from all Swine People Ranch at Polewali in Polewali Mandar district, West Sulawesi. The samples made into an blood thinner then examined under a microscope. From 80 samples, the results showed that there are 4 samples which is positive infested Theileria sp. Key words : Blood Parasite, Swine, Theileria, Theileriosis.
An In Vitro Antibacterial Activity Test of Meniran Herbs’(Phyllanthus Niruri L.) Ethanol ExtractAgainst Mycoplasma gallisepticum causes Chronic Respiratory Disease (CRD) in Broiler Chickens
Authors: Sabdoningrum Emy Koestanti , Hidanah Sri , Wahjuni Retno Sri , Chusniati Sri , Arimbi Arimbi 
Published: March 2018

Chronic Respiratory Disease (CRD) is a chicken respiratory disease that attacks both broilers and layers. Chronic Respiratory Disease (CRD) has important economic significance in the intensification of chicken farms because this disease can cause huge economic losses. Meniran plant (Phyllanthus niruri Linn) is one of the plants that can be used as prevention and alternative treatment as a substitute of antibiotic caused by Mycoplasma galisepticum causes Chronic Respiratory Disease (CRD) in broiler chickens. The chemicals contained in meniran include tannins, saponins, alkaloids as antibacterials. The purpose of this study is to determine the activity of meniran herbs’ (Phyllanthus Niruri Linn) as antibacterial to eradicate Mycoplasma galisepticum. The method of this study is dilution method which included Minimum Inhibitory Concentration [MIC) and Minimum Bactericidal Concentration (MBC). Minimum Inhibitory Concentration [MIC) was taken by making the concentration of meniran extract as much 65%, 62,5%; 60%; 55%; 50%; 45%; 40%. It was then added Mycoplasma gallisepticum bacteria. The result of this study is Meniran’s activation test on Mycoplasma galisepticum obtained a dose of 62,5% could eradicate Mycoplasma galisepticum causes Chronic Respiratory Disease (CRD) in broiler chickens. Meniran herbs’ (Phyllanthus niruri linn) is effective as antibacterial at concentrations of 30% against Mycoplasma gallisepticum causes Chronic Respiratory Disease (CRD) in broiler chickens. Keywords: Meniran herbs’ (Phyllanthus Niruri Linn), Mycoplasma Galisepticum, Chronic Respiratory Disease (CRD)
Bioremediation of Mercury (II) Contaminated Seawater Using the Diatom Skeletonema costatum
Authors: Soedarti Thin , S. Tini , H. Sucipto , Kuncoro Eko P. 
Published: March 2018

The mercury contaminated seawater can pollute fish pond. Bioremediation is an effective process for the removal and recovery of mercury (II) from seawater using organism as an agent of biological degradation. The aim of this study was to know the optimum contact time and concentrations of the Skeletonema costatum cell inoculation on the bioremediation in mercury (II) contaminated seawater. This study has used the concentrations of the cell inoculation (5000; 10000; and 15000 cells/mL), the mercury (II) (0; 0.5; 1; and 2 mg/L), the contact time (24, 48, 72, 96, and 120 hours), and its replicated five times. The maximum bioremediation capacity of mercury (II) was 2 mg/L at 15 000 cells/mL and contact time 96 hours, with bioremediation efficiency 86.83%. Diatom Skeletonema costatum was efficient at removing 2 mg/L mercury (II) 79.5% for 5 000 cells/L at 72 hours, 83.3 % for 10 000 cells/L at 72 hours and 85% for 15 000 cells/L at 72 hours. The optimum contact time and concentrations of the Skeletonema costatum cell inoculation on the bioremediation in mercury (II) contaminated seawater for 2 mg/L, i.e. 5 000 cells/L for 72 hours (79.5%) Keywords: bioremediation; mercury (II); seawater; Skeletonema costatum.
BMP-2 Expression of Post Tooth Extraction that Catfish Oil Application
Authors: B. Theresia Indah , Sumaryono Bambang , Suardita Ketut , R. Amelia Putri 
Published: March 2018

Catfish (Clarias batrachus) oil contain the highest amount of omega-3-PUFA among other freshwater fish. The omega-3-PUFA in fish oil produced eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is known that EPA and DHA in essentials fatty acid (EFA) could improve BMP-2 expression. Bone morphogenetic protein-2 (BMP-2) is bone stimulator which capable of inducing differentiation of mesenchymal cells into osteoblast, stimulating bone formation in wound healing process of dental extraction. Purpose: To prove the increasing expression of BMP-2 after catfish (Clarias batrachus) oil application. Methods: We have used the post test only design in this research. There have been 21 Rattus novergicus as research samples, and those were divided into 3 groups, group KK as control, group KP1 was given catfish (Clarias batrachus) oil in 5% concentration, and group KP2 was given catfish (Clarias batrachus) oil in 10% concentration. Catfish (Clarias batrachus) oil were applied into the socket of dental extraction. Rat was decapulated 7 days after fish oil application and the jaw in the treated regions and control group were cut for immunohistochemistry examination to observe BMP-2 expression. Data was analyzed using one-way ANOVA test. Result: There is significant difference increased of BMP-2 expression between control and treatment group. In samples given with 10% concentration of catfish oil had the most significant increase of BMP-2 expression. Conclusion: Catfish (Clarias batrachus) oil in 10% concentration could increase the expression of BMP-2 post dental extraction.Key words: Catfish oil, BMP-2, dental extraction.
Inhibition of Apoptosis in Retinal of Newborn Mice Due to Congenital Toxoplasmosis
Authors: Suwanti Lucia Tri , Mufasirin Mufasirin , Plumeriastuti Hani 
Published: March 2018

Toxoplasma gondii infection in pregnant women cause defects in the newborn, such as hydrocephalus and eye damaged, even blindness. Histologically damage due to congenital infection of T. gondii need to be examined. Twenty pregnant mice were divided into two groups which are the treatment group and the control group. Each mouse in treatment group was infected with 10 takizoit by intraperitoneal. Each of the newborn were sacrified, their head were taken and their eye tissue were fixed in 10% of buffered formalin and the histological sample were made in HE and TUNEL staining. The result showed that the retina of the eye of the newborn from infected mice damage. The damages include: hemorrhage, infected retinal cells, eye growth inhibition and decreased of apoptosis index of the retina cells. Keywords: Apoptosis in retinal, Newborn, Congenital Toxoplasmosis, Ocular Toxoplasmosis
Effect of Spirulina Platensis on The Number of Spermatogenic Cells in The Seminiferous Tubules of Rat (Rattus Norvegicus) with Excessive Physical Exercise
Authors: Rosidawati Rahmah Wahyu , Rimayanti Rimayanti , Supranianondo Koesnoto 
Published: March 2018

The aim of this study was to investigate the effect of Spirulina platensis extract on the number of spermatogenic cells of rats with excessive physical exercise. Excessive physical exercise was achieved through one hour of swimming for 35 days. Twenty male rats were divided into five groups i.e (1) C-, control group, (2) C+, a group was only given swimming, (3) T1, a group was given 300 mg/kg BW of Spirulina platensis and swimming, (4) T2, a group was given 600 mg/kg BW of Spirulina platensis and swimming, and (5) T3, a group was given 1200 mg/kg BW of Spirulina platensis and swimming. Spirulina platensis extract was given orally once a day before swimming. ANOVA test followed with Duncan test showed that the number of spermatogenic cells significantly different among treatments (p<0.05). Excessive physical exercise was able to influence on the male reproduction system by declined on the number of spermatogenic cells in seminiferous tubules of male rat. The conclusion of this study was dose of 1200 mg/kg BW of Spirulina platensis extract could maintain the number of spermatogenic cells of male rat after excessive physical exercise. Keywords : Rattus norvegicus, Spirulina platensis, excesive physical exercise, spermatogenic cells
Phytochemicals, Antioxidant and Antifungal Properties of Acorus calamus, Curcuma mangga, and Allium sativum
Authors: Muchtaromah Bayyinatul , Ahmad Mujahidin , S Emy Koestanti , A Yuni Ma’rifatul , A Velayati Labone 
Published: March 2018

The purpose of this study to determine the content of phytochemicals, antioxidant and antifungal properties of the combination of Acorus calamus, Curcuma mangga, and Allium sativum. This research was descriptive qualitative, extractions were done by maceration method with ethanol with 3 different combinations (C1, C2 and C3). Phytochemical test reagent included 4 kinds of test, namely: alkaloids, flavonoids, triterpenoids, saponins and tannins. As for the antioxidant test, the method used was DPPH. The concentration used at 25, 50, 100, 200, and 400 ppm. As for the antifungal test conducted on Candida albicans with Kirby-Bauer disc methods with a concentration of 100%, followed by the MIC and MBC test with a concentration of 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78% and 0.39%. Phytochemical test results indicated the presence of the alkaloids, flavonoids and triterpenoids compounds in 3 different combinations (C1, C2 and C3). The highest antioxidant levels founded in C1 (61.75) followed by C3 (47.94) and the lowest levels founded in C2 (42.76). The antifungal test showed the inhibitory zone against C. albicans. The highest inhibitory zone was found in C1 at 5.44 ± 1.78 mm (medium category), followed by C2 at 4.08 ± 0.86 mm (medium category), and C3 at 3.05 ± 0.23 mm (medium category). As for the minimum inhibitory concentration (MIC) value got on the concentration of 0:39% and minimum fungisidal concentration (MFC) values were at a concentration of 0.78%. Key word: Acorus calamu, Allium sativum, Curcuma manga, Antioxidant, antifungal, Candida albicans
Shark Species on Export Products from East Java and Bali by Dna Barcoding Based on Internal Transcribed Spacer-2 (Its-2) Locus in Mitochondrial
Authors: Aksono Eduardus Bimo 
Published: March 2018

DNA barcoding method is the mitochondrial marker for all animal species, and it is claimed as distinguishing feature from one species to another Species identification in shark products is often difficult to perform as they have morphological similarities with many other species and it is even more difficult as they are parts separated from the body for the storage. This research is aimed to know which species of sharks identified in the export products from East Java and Bali by DNA barcoding method. The samples of sharks (meat, fins, skin and bones) used were 90 samples acquired in Surabaya from the export products of East Java and Bali from 2015 to 2017. The DNA barcoding method uses universal primer through nested PCR (Polymerase Chain Reaction) process which is able to amplify the DNA until around 1,340 bp based on Internal Transcribed Spacer-2 (ITS-2) locus of mitochondria. Based on the result of phylogenetic analysis and the classification list by IUCN, from 90 samples of sharks acquired from export products in East Java and Bali, species identified were: 1.11% Daenia sp categorized as NE (not evaluated); 4.44% Sphyrna zygaena categorized as NT (not Threatened); 3.33% Sphyrna lewini categorized as NT (not Threatened); 10% Rhizoprionodon taylori categorized as LC (least concern); 24% Charcarhinus brevipinna categorized as NT (not Threatened); 2.22% Charcarhinus obscurus categorized as NT (not Threatened); 3.33% Charcarhinus falciformis categorized as LC (least concern); 1.11% Charcarhinus plumbeus categorized as NT (not Threatened); 27.78% Charcarhinus longimanus categorized as VU (vulnerable); 1.11% Neutrygon kuhlii categorized as NE (not evaluated); 1.11% Charcarhinus Taurus categorized as VU (vulnerable); 3.33% Rhizoprionodon longurio categorized as DD (data deficient); 1.11% Rhizoprionodon porosus categorized as DD (data deficient); 1.11% Eusphyra blochii categorized as NT (not Threatened); 4.44% Chiloscyllium griseum categorized as DD (data deficient); 5.56% Rhizoprionodon oligolinx categorized as LC (least concern); 1.11% Prionace glauca categorized as NT (not Threatened); 1.11% Rhizoprionodon lalandii categorized as DD (data deficient). Generally all species found in this research were special fish from Indo-Australian archipelago and included in IUCN red list. The government policy to prohibit export of these species was the right decision to prevent the species extinction. Keywords : ITS-2; Shark; Export Products; Java and Bali
Bioactivity of human Menopausal Gonadotrophin (hMG) and Deglycosylated hMG (hMGdG) from Urine of Post-Menopausal Women On invitro Bovine Embryonic cleavage
Authors: Hermadiv Herry Agoes 
Published: March 2018

The aim of this study was to produce hMG from the uterus of post-menopausal women and to evaluate the influence of glycan removal from hMG glycoprotein molecule underwent deglycosylation (hMGdG) on the onset of in vitro bovine embryonic cleavage. The study identified hMG from the uterus of post-menopausal women by confirmation of the glycoprotein characteristic, examined the biochemical characteristics of deglycosylated hMG using N-glycanase and determined the influence of deglycosylated hMG on the onset of in vitro bovine embryonic cleavage. Urine samples were collected from 30 post-menopausal women. The results of SDS-PAGE demonstrated that the protein bands ranged between 19.4 and 107 kDa. Western blot revealed immune-reactivity of the 30 kDa band, which was a glycoprotein. The concentration of glycoprotein was 99860.00 ug/ml (PAS), the protein was 66939.29 µg/ml (Biuret) and carbohydrate 32920.71 µg/mL (PAS). The glycoprotein, protein and carbohydrate ratio within the hMG molecule was 3:2:1. Chemical characteristic of hMG following enzymatic deglycosylation using N-glycanase reduced the molecular weight to 26 kDa. The deglycocylated hMG reduced the onset of in vitro cleavage of bovine embryo from 24 to 20 hours (p < 0.01). Keywords: hMG, hMGdG, embryo cleavage, glycoprotein, deglycocylation, in vitro
The Effect of Frequency Acoustic Stimulation Sound on Intrauterine Weakening of Pregnant Sheep
Authors: Suherman Djamil , Joewono Hermanto Tri , Sardjana I Komang Wiarsa 
Published: March 2018

Research to determine changes in intrauterine sound pressure in pregnant sheep after administration acoustic stimulation outside of the abdominal wall at some frequency sounds. The study was conducted at the Animal Hospital of Veterinary Faculty of Airlangga University. Pre test experimental design with pre- and post-test one group to assess intra-uterine sound pressure changes. The study was conducted at two lambs pregnant aterm after acoustic stimulation at a distance of 10 cm from the surface of the abdominal wall to the sound pressure 80,85,90,95 and 100 decibels and sound frequency of 31.5, 63, 125, 250, 500, 1000, 2000, 4000, 6000 and 8000 hertz. The results showed that the difference between the sound pressure outside of the abdominal wall with intrauterine sound pressure on both the pregnant sheep by an average of 16.7570 ± 8.0797 decibels. This shows their weakening sound after passing through the abdominal wall and the uterine wall. By using a paired t-test, this weakening statistically significant. At frequencies from 31.5 to 1000 hertz weakening values from 5.2 to 17.1 decibels while in 2000-8000 hertz frequency weakening value of 20.2 to 30.8 decibels. The conclusion that the stimulation of noise from outside the walls of the abdomen weakening sound after penetrating the abdominal wall and the uterine wall. Weakening occur at every level of sound pressure and at every level of a given frequency. Weakening value becomes greater at frequencies above 1000 hertz. Keywords: weakening, sound pressure, sound frequency, pregnant sheep.
A Retrospective Study of Canine Pyometra in Segar Veterinary Hospital, Kuala Lumpur, Malaysia Year 2012-2016
Authors: Hui Ng Xin , Hariadi Mas’ud , Primarizky Hardany 
Published: March 2018

A retrospective study was used to analyse canine pyometra cases in Segar Veterinary Hospital, Kuala Lumpur, Malaysia from May 2012 to May 2016 and to investigate the relationship between pyometra and breed and age of dogs. The study was done through secondary collection of data from ambulatoirs of pyometra cases which were diagnosed based on anamnesis, examination of clinical signs and ultrasonography and/or radiography. The data collected includes breed categorised into small, medium, and large breeds, whereas the age are categorised into puppies, adulthood and geriatric. The data was then analysed with tree classification analysis and CATPCA (Principal Components Analysis for Categorical Data) analysis using SPSS program. A total of 80 cases of pyometra were recovered from female dog patients over the study period. Small breed dogs at 72.5% (n=58) and geriatric dogs at 62.5% (n=50) had the highest percentage of pyometra. The breeds Mongreal, German Shepard Dog, Mini Schnauzer, Silky Terrier, Toy Poodle, Beagle, Chow Chow, Golden Retriever, Rottweiler, Cocker Spaniel, White Terrier, Siberian Husky, and Pekingese aged older than 5.5 years had 100% from 37 cases of open-cervix pyometra. Geriatric and small breed dogs are inclined to have open-cervix pyometra. However adult and medium or large breed dogs have a higher possibility to have closed-cervix pyometra. These results serve to highlight the importance of public awareness regarding canine pyometra and further researches are needed to find out the effects of hormone therapy, frequency of births, and the bacteria present in uterus with pyometra. Keywords: Canine pyometra, Open-cervix Pyometra, Closed-cervix Pyometra, Age and Breed
Oocyte Quality and Subsequent In Vitro Maturation of Sheep Oocyte-Cumulus Complex from Ovary with Presence and Absence of Corpus Luteum
Authors: Widyastuti Rini , Syamsunarno Mas Rizky A.A. , Saili Takdir , Boediono Arief 
Published: March 2018

In vitro maturation is the crucial step for in vitro embryo production. It needs a large number of oocytes as source gamet cells recovered. The present study is aimed to assess the influence of corpus luteum on the average number oocytes harvested, COCs quality and subsequent maturation of immature oocytes recovered from sheep ovaries. Sheep ovaries were collected from local slaughterhouse and COCs were collected by using slicing method. Collected COCs were graded into three categories dependent upon cumulus cells surrounding them and the homogenous of cytoplasm. COCs were maturated in maturation media at 5% CO2 for 24 hours. Maturation of oocytes evaluated base on the expansion of cumulus cells and extrusion of the first polar body. There was significantly higher on average of COCs harvested from ovaries with corpus luteum compared without corpus luteum. The presence of Corpus luteum did not affect the COCs quality and ability to reach the maturation stage. However, there was a dramatic effect of cultured COCs quality on maturation rate both groups. Collectively, these results indicate that COCs quality is the main factor affecting the subsequent of oocytes matured in vitro. Keywords: Corpus luteum; cumulus oocyte complex; in vitro maturation; maturation rate; ovaries
The Effect of Insulin-Like Growth Factor-I of Pregnant Crossbred Mare Serum and Insulin-Like Growth Factor-I Recombinant Mouse on Estrous Cycles and Litter Sizes of Mice (Mus musculus)
Authors: Restiadi Tjuk Imam , Mustofa Imam , Utama Suzanita , Mulyati Sri 
Published: March 2018

The research was conducted to determine the effect of insulin-like growth factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) on estrous cycle and litter size in mice (Mus musculus). The research was arranged by Completely Randomized Design (CRD) with seven treatments and five replications. The treatments were control (CO)= physiological NaCl, treatment (T) 1 = 10 ng/ml of IGF-I pregnant mares crossbred serum (PMS), T2 = 20 ng/ml of IGF-I PMS, T3 = 40 ng/ml of IGF-I PMS, T4 = 10 ng/ml of IGF-I recombinant mouse, T5 = 20 ng/ml of IGF-I recombinant mouse, and T6 = 40 ng/ml of IGF-I recombinant mouse. Serie I the subject of this research were 35 female mice. Observed variables included estrous cycle phases that proestrus, estrus, metestrus, and diestrus phase. Serie II for the objects of this research were 35 female mice and 21 male mice in the same treatment on litter size in mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS did not significantly affect (p>0.05) on proestrus, metestrus, and diestrus phase. The addition of IGF-I PMS 40 ng/ml was extended (p<0.05) the estrus phase. It showed that there was not significantly difference (p>0.05) between the effect of IGF-I PMS and IGF-I recombinant mouse with the mice estrous cycles. The result of IGF-I derived from PMS and IGF-I recombinant mouse on litter size in mice did not significantly difference (p>0.05). Test results shows significant differences between control and 40 ng/ml of IGF-I PMS. Keywords: IGF-I, IGF-I pregnant crossbred mare serum, estrous cycle, litter sizes, Mus musculus
Adding of L-Arginin Amino Acidin Skim Milk Diluent to Maintain Quality of Buck Sperm in Cold Temperature
Authors: Suprayogy Tri Wahyu , Susilowati Suherni , Hernawati Tatik 
Published: March 2018

Nowdays, the storage of buck semen in cold temperature have not satisfied yet because in buck’s seminal plasma contains phospholipase enzyme which can coagulated egg yolk in diluents.The specific aim of this study was to investigate the benefits of L-Arginin amino acid in skim diluents to quality buck’s spermatozoa on cold temperature. This researchutilized four treatment groups, namely Controlled group (P0): skim milk diluent without L-Arginin + buck’s semen; P1: skim milk diluents + L-Arginin 0,002M/ml + buck” semen; P2: skim milk diluents + L-Arginin 0,004 M/ml + buck’s semen and P3: skim milk diluents + L-Arginin 0,006 M/ml + buck’s semen. Then the samples stored in cold temperature (5oC). The result showed that sperm motility, viability and membrane integrity were significantly different (p<0,05) among the treatments.The conclusion of this study is adding of L-Arginin Amino Acid in skim milk diluents maintain motility, viability and membrane integrity buck’s sperm. Keywords: L-Arginin, buck, cold temperature, motility, viability and membrane integrity