bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human factors awareness and recognition during multidisciplinary team meetings
Authors: Oeppen Rachel S. , Davidson Mike , Scrimgeour Duncan S. , Rahimi Siavash , Brennan Peter A. 
Published: March 2019

AbstractMultidisciplinary team (MDT) meetings are widely used throughout medicine and dentistry, bringing together expertise and different opinions across many disciplines to benefit patient care. Depending on the cancer site and specialties involved, some MDTs can last for several hours, especially if there are many complex patients to discuss. However, concentration and attention can vary and distraction is almost inevitable with separate conversations between MDT members and the ever‐increasing use of smartphones. The role of human factors (HF) in contributing to error is well known in high‐risk activities including medicine and surgery. Surprisingly, while there is increasing awareness of their importance by medical and dental professionals to enhance patient safety, to our knowledge nothing to date has been published about the possible effect and role of HF at MDTs. Here we provide a brief HF overview and focus on the factors at an MDT that could lead to distraction, providing suggestions (including some from aviation) for possible ways to enhance and improve discussion during these often‐long meetings. It is hoped that this paper will generate some thought and discussion around the current “normal” MDT practice in head and neck and other specialties and challenge colleagues to embrace HF and safety principles in a just and learning culture.
Activation of melatonin receptor 2 but not melatonin receptor 1 mediates melatonin‐conferred cardioprotection against myocardial ischemia/reperfusion injury
Authors: Han Dong , Wang Yongjun , Chen Jiangwei , Zhang Jibin , Yu Peng , Zhang Ran , Li Shuang , Tao Bo , Wang Yabin , Qiu Ya , Xu Mengqi , Gao Erhe , Cao Feng 
Published: March 2019

AbstractAccumulated pieces of evidence have proved the beneficial effects of melatonin on myocardial ischemia/reperfusion (MI/R) injury, and these effects were largely dependent on melatonin membrane receptor activation. In humans and other mammals, there are two types of melatonin receptors, including the melatonin receptor 1 (MT1, melatonin receptor 1a or MTNR1A) and melatonin receptor 1 (MT2, melatonin receptor 1b or MTNR1B) receptor subtypes. However, which receptor mediates melatonin‐conferred cardioprotection remains unclear. In this study, we employed both loss‐of‐function and gain‐of‐function approaches to reveal the answer. Mice (wild‐type; MT1 or MT2 silencing by in vivo minicircle vector; and those overexpressing MT1 or MT2 by in vivo AAV9 vector) were exposed to MI/R injury. Both MT1 and MT2 were present in wild‐type myocardium. MT2, but not MT1, was essentially upregulated after MI/R Melatonin administration significantly reduced myocardial injury and improved cardiac function after MI/R Mechanistically, melatonin treatment suppressed MI/R‐initiated myocardial oxidative stress and nitrative stress, alleviated endoplasmic reticulum stress and mitochondrial injury, and inhibited myocardial apoptosis. These beneficial actions of melatonin were absent in MT2‐silenced heart, but not the MT1 subtype. Furthermore, AAV9‐mediated cardiomyocyte‐specific overexpression of MT2, but not MT1, mitigated MI/R injury and improved cardiac dysfunction, which was accompanied by significant amelioration of oxidative stress, endoplasmic reticulum stress, and mitochondrial dysfunction. Mechanistically, MT2 protected primary cardiomyocytes against hypoxia/reoxygenation injury via MT2/Notch1/Hes1/RORα signaling. Our study presents the first direct evidence that the MT2 subtype, but not MT1, is a novel endogenous cardiac protective receptor against MI/R injury. Medications specifically targeting MT2 may hold promise in fighting ischemic heart disease.
Genetic analysis of the Linnaean Ulva lactuca (Ulvales, Chlorophyta) holotype and related type specimens reveals name misapplications, unexpected origins, and new synonymies
Authors: Hughey Jeffery R. , Maggs Christine A. , Mineur Frédéric , Jarvis Charlie , Miller Kathy Ann , Shabaka Soha Hamdy , Gabrielson Paul W. 
Published: March 2019

Current usage of the name Ulva lactuca, the generitype of Ulva, remains uncertain. Genetic analyses were performed on the U. lactuca Linnaean holotype, the U. fasciata epitype, the U. fenestrata holotype, the U. lobata lectotype, and the U. stipitata lectotype. The U. lactuca holotype is nearly identical in rbcL sequence to the epitype of U. fasciata, a warm temperate to tropical species, rather than the cold temperate species to which the name U. lactuca has generally been applied. We hypothesize that the holotype specimen of U. lactuca came from the Indo‐Pacific rather than northern Europe. Our analyses indicate that U. fasciata and U. lobata are heterotypic synonyms of U. lactuca. Ulva fenestrata is the earliest name for northern hemisphere, cold temperate Atlantic and Pacific species, with U. stipitata a junior synonym. DNA sequencing of type specimens provides an unequivocal method for applying names to Ulva species.
An outback oasis: the ecological importance of bilby burrows
Authors: Dawson S. J. , Broussard L. , Adams P. J. , Moseby K. E. , Waddington K. I. , Kobryn H. T. , Bateman P. W. , Fleming P. A. 
Published: March 2019

AbstractEcosystem engineers are species that have a role in creating and maintaining certain habitat traits that are important for other species. Burrowing species do this by creating subterranean refugia from predation and thermal extremes, but also providing foraging opportunities through soil movement and by increasing local landscape heterogeneity. In this study, we used camera traps to monitor the burrows of greater bilbies (Macrotis lagotis), a vulnerable Australian marsupial, in an area subject to frequent disturbance by fire. We tested the hypothesis that bilby burrows provide refuge for other species and therefore their presence increases biodiversity. In total, 45 taxa – 22 bird, 16 reptile and 7 mammal taxa – were recorded interacting with 127 burrows across 7 sites. Species richness was greater at burrows compared with vegetation away from burrows, while abundance was no different. There was no difference in species assemblage for bilby burrows that were actively maintained by bilbies compared with abandoned burrows, although there was more activity at bilby maintained burrows. A wildfire allowed us to test the ad hoc hypothesis that the use of bilby burrows was greater when vegetation cover was removed by fire. We recorded significant differences in species assemblage interacting with burrows after fire, although overall species richness and abundance did not change. The response of individual species was variable; for example, burrows provide a refuge for smaller species (such as mice and small reptiles), and may therefore protect them from the effects of fire. Where they persist, bilbies provide an important ecosystem engineering service, as their burrows support a broad range of species. Further reduction in the distribution of the bilby is therefore likely to have a flow‐on effect on biodiversity, impacting species that use their burrows for refuge.
Geochemistry of a confirmed Precambrian impact ejecta deposit: The Grænsesø spherule layer, South Greenland
Authors: Huber Matthew S. , Koeberl Christian , Smith Frank C. , Glass Billy P. , Mundil Roland , McDonald Iain 
Published: March 2019

AbstractSamples from a single outcrop of the Graenseso spherule layer, Midternaes, South Greenland, consist of a spherule‐bearing dolomixtite with matrix‐supported intraclasts up to 1 m in size. In addition to field observations, we performed mineralogical and whole rock geochemical analysis, including electron microprobe, neutron activation analysis, X‐ray fluorescence, and mass spectrometry of the horizon and the overlying and underlying strata. We show that the spherules are petrographically similar to those in the Zaonega spherule layer, Karelia, Russia. Our petrographic and chemical results are consistent with the previous suggestion that the Grænsesø layer correlates with the Zaonega layer, and it is possible that both layers are related to the Vredefort impact event. The samples containing spherules, as well as the overlying rocks, have elevated REEs compared to the underlying pre‐impact layer, suggestive of a new continental source of sediment that may be coincident with the impact event. Zircons separated from the lower part of the Grænsesø spherule layer display complex age patterns suggesting that they have genetically different origins based on distinctly different Th/U ratios. Crystallization ages of all groups are ≥ 2.8 Ga, with ~2.8 Ga representing a time of major crustal growth globally. Therefore, we cannot conclusively determine in this study if the zircons are locally derived or if they are transported with the ejecta. The spherule layer was deposited by a high‐energy, subaqueous debris flow, an origin that is consistent with the hypothesis that the layer was deposited by impact‐induced waves and/or currents.
Linear growth faltering and the role of weight attainment: Prospective analysis of young children recovering from severe wasting in Niger
Authors: Isanaka Sheila , Hitchings Matt D.T. , Berthé Fatou , Briend André , Grais Rebecca F. 
Published: March 2019

AbstractEfforts to reduce the impact of stunting have been largely independent of interventions to reduce the impact of wasting, despite the observation that the conditions can coexist in the same child and increase risk of death. To optimize the management of malnourished children—who can be wasted, stunted, or both—the relationship between stunting and wasting should be elaborated. We aimed to describe the relationship between concurrent weight and height gain during and after rehabilitation from severe wasting. We conducted a secondary analysis of a randomized trial for the outpatient treatment of severe wasting, including 1,542 children who recovered and were followed for 12 weeks. We described the overlap of stunting and severe wasting and the change in stunting over time. We showed the relationship between concurrent weight and height gain using adjusted generalized estimating equations and calculated the mean rate of change in weight‐for‐height z score (WHZ) and height‐for‐age z score (HAZ) during and after rehabilitation. At baseline, 79% (n = 1,223/1,542) and 49% (n = 757/1,542) of children were stunted and severely stunted, respectively. Prevalence increased over time among children <24 months. During rehabilitation when weight was not yet fully recovered, we found rapid WHZ gain but limited HAZ gain. Following successful rehabilitation, WHZ gain slowed. The rate of HAZ gain was negative after rehabilitation but increased relative to the period during treatment. The potential relationship between weight and height gain calls for increased coverage of wasting treatment to not only prevent child mortality but also reduce linear growth faltering.
Exomic and transcriptomic alterations of hereditary gingival fibromatosis
Authors: Han Seong Kyu , Kong Jungho , Kim Sanguk , Lee Jae‐Hoon , Han Dong‐Hoo 
Published: March 2019

AbstractObjectiveHereditary gingival fibromatosis (HGF) is a rare oral disease characterized by either localized or generalized gradual, benign, non‐hemorrhagic enlargement of gingivae. Although several genetic causes of HGF are known, the genetic etiology of HGF as a non‐syndromic and idiopathic entity remains uncertain.Subjects and methodsWe performed exome and RNA‐seq of idiopathic HGF patients and controls, and then devised a computational framework that specifies exomic/transcriptomic alterations interconnected by a regulatory network to unravel genetic etiology of HGF. Moreover, given the lack of animal model or large‐scale cohort data of HGF, we developed a strategy to cross‐check their clinical relevance through in silico gene–phenotype mapping with biomedical literature mining and semantic analysis of disease phenotype similarities.ResultsExomic variants and differentially expressed genes of HGF were connected by members of TGF‐β/SMAD signaling pathway and craniofacial development processes, accounting for the molecular mechanism of fibroblast overgrowth mimicking HGF. Our cross‐check supports that genes derived from the regulatory network analysis have pathogenic roles in fibromatosis‐related diseases.ConclusionsThe computational approach of connecting exomic and transcriptomic alterations through regulatory networks is applicable in the clinical interpretation of genetic variants in HGF patients.
Safety and efficacy of anti‐tachycardia pacing in patients with hypertrophic cardiomyopathy implanted with an ICD
Authors: Adduci Carmen , Semprini Lorenzo , Palano Francesca , Musumeci Maria Beatrice , Volpe Massimo , Autore Camillo , Francia Pietro 
Published: March 2019

AbstractBackgroundIn hypertrophic cardiomyopathy (HCM) patients implanted with an implantable cardioverter defibrillator (ICD), clinical outcomes of antitachycardia pacing (ATP) have been poorly explored. In a retrospective analysis of a cohort of consecutive HCM patients implanted with an ICD, we aimed to assess the efficacy, safety, and clinical value of ATP.MethodsThe cohort of HCM patients implanted with a transvenous ICD and followed in our center was assessed for device intervention from implantation to last clinical follow‐up.ResultsOverall 77 patients (45 males; mean age: 46 ± 16 years) were analyzed. After 67 ± 41 months, 24 patients had 49 ventricular tachycardia/fibrillation (VT/VF) appropriately treated (5.8% per year). Among 39 monomorphic VTs, ATP was effective in 27 (success rate: 69%). Mean time from VT onset to ATP delivery was 9.1 ± 4.9 s. The only clinical variable improving ATP success was use of beta‐blockers (81% vs 50%; P = .04). Out of 12 ineffectively treated VTs, one was immediately shocked, four self‐terminated after 18 ± 12 s, and seven (18%) were accelerated to a new VT. ATP was also delivered for 27 of 42 inappropriately detected episodes and induced two de novo VTs (7%). In the per patient analysis, 14 out 77 (18%) patients had one or more appropriate and effective ATP (3.4% per year), and only six (8%; 1.4% per year) received more than one ATP.ConclusionATP is moderately effective for the treatment of monomorphic VTs in HCM patients. However, the rate of appropriate ATP therapies is low, ATP is often prematurely delivered, and ATP‐induced arrhythmia degeneration is of concern.
Quantifying the completeness of the bat fossil record
Authors: Brown Emily E. , Cashmore Daniel D. , Simmons Nancy B. , Butler Richard J. 
Published: March 2019

AbstractBats (Chiroptera) are one of the most successful extant mammalian orders, uniquely capable of powered flight and laryngeal echolocation. The timing and evidence for evolution of their novel adaptations have been difficult to ascertain from the fossil record due to chronological gaps and the fragmentary nature of most fossil bat material. Here, we quantify the quality of the bat fossil record using skeletal and character completeness metrics, which respectively document for each taxon what proportion of a complete skeleton is preserved, and the proportion of phylogenetic characters that can be scored. Completeness scores were collected for 441 valid fossil bat species in 167 genera from the Eocene to the Pleistocene. All metrics record similar temporal patterns: peak completeness in the Lutetian stage reflects the presence of Lagerstätten, while subsequent stages have very low completeness, except an Aquitanian high and a Pleistocene peak in skeletal completeness. Bat completeness is not correlated with intensity of sampling through geological time but has a weak negative correlation with publication date. There is no correlation between taxonomic richness and completeness, as the bat record predominately consists of diagnostic but isolated teeth. Consequently, bat skeletal completeness is the lowest of any previously assessed tetrapod group, but character completeness is similar to parareptiles and birds. Bats have significantly higher character completeness in the northern hemisphere, probably due to heightened historical interest and presence of Lagerstätten. Taxa derived from caves are more complete than those from fluviolacustrine and marine deposits, but do not preserve highly complete specimens.
CRISPR‐LbCas12a‐mediated modification of citrus
Authors: Jia Hongge , Orbović Vladimir , Wang Nian 
Published: March 2019

SummaryRecently, CRISPR‐Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR‐LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully in Duncan grapefruit via Xcc‐facilitated agroinfiltration. Next, LbCas12a driven by either the 35S or Yao promoter was used to edit the PthA4 effector binding elements in the promoter (EBEPthA4‐CsLOBP) of CsLOB1. A single crRNA was selected to target a conserved region of both Type I and Type II CsLOBPs, since the protospacer adjacent motif of LbCas12a (TTTV) allows crRNA to act on the conserved region of these two types of CsLOBP. CsLOB1 is the canker susceptibility gene, and it is induced by the corresponding pathogenicity factor PthA4 in Xanthomonas citri by binding to EBEPthA4‐CsLOBP. A total of seven 35S‐LbCas12a‐transformed Duncan plants were generated, and they were designated as #D35s1 to #D35s7, and ten Yao‐LbCas12a‐transformed Duncan plants were created and designated as #Dyao1 to #Dyao10. LbCas12a‐directed EBEPthA4‐CsLOBP modifications were observed in three 35S‐LbCas12a‐transformed Duncan plants (#D35s1, #D35s4 and #D35s7). However, no LbCas12a‐mediated indels were observed in the Yao‐LbCas12a‐transformed plants. Notably, transgenic line #D35s4, which contains the highest mutation rate, alleviates XccΔpthA4:dCsLOB1.4 infection. Finally, no potential off‐targets were observed. Therefore, CRISPR‐LbCas12a can readily be used as a powerful tool for citrus genome editing.
Elevated CO2 does not affect stem CO2 efflux nor stem respiration in a dry Eucalyptus woodland, but it shifts the vertical gradient in xylem [CO2]
Authors: Salomón Roberto L. , Steppe Kathy , Crous Kristine Y. , Noh Nam Jin , Ellsworth David S. 
Published: March 2019

AbstractTo quantify stem respiration (RS) under elevated CO2 (eCO2), stem CO2 efflux (EA) and CO2 flux through the xylem (FT) should be accounted for, because part of respired CO2 is transported upwards with the sap solution. However, previous studies have used EA as a proxy of RS, which could lead to equivocal conclusions. Here, to test the effect of eCO2 on RS, both EA and FT were measured in a free‐air CO2 enrichment experiment located in a mature Eucalyptus native forest. Drought stress substantially reduced EA and RS, which were unaffected by eCO2, likely as a consequence of its neutral effect on stem growth in this phosphorus‐limited site. However, xylem CO2 concentration measured near the stem base was higher under eCO2, and decreased along the stem resulting in a negative contribution of FT to RS, whereas the contribution of FT to RS under ambient CO2 was positive. Negative FT indicates net efflux of CO2 respired below the monitored stem segment, likely coming from the roots. Our results highlight the role of nutrient availability on the dependency of RS on eCO2 and suggest stimulated root respiration under eCO2 that may shift vertical gradients in xylem [CO2] confounding the interpretation of EA measurements.
Focus stacking photogrammetry for micro‐scale roughness reconstruction: a methodological study
Authors: Olkowicz Marcin , Dabrowski Marcin , Pluymakers Anne 
Published: March 2019

AbstractIn close‐range applications, it is unclear whether optical photogrammetry is capable of accurately reconstructing submillimetre‐scale roughness. This paper presents a study of fine‐scale rock fracture roughness measurements with a careful assessment of the error. The workflow combines the techniques of structure from motion with focus stacking, using consumer‐grade equipment and free or affordable software. The approach is tested firstly with synthetic data to check the influence of the number and position of cameras, object texture and image processing using focus stacking. Secondly, the optimised workflow is used to measure a natural shale rock fracture surface. To estimate the accuracy, the results were compared with a high‐precision reference dataset provided by white‐light interferometry. The standard deviation of error in the method is 6·5 μm, and is related with morphological structures with wavelengths below 150 μm and amplitudes smaller than 10 μm.
Is there evidence for post‐epidemic attenuation in the Dutch elm disease pathogenOphiostoma novo‐ulmi?
Authors: Brasier C. M. , Webber J. F. 
Published: March 2019

The first pandemic of Dutch elm disease (DED) in Europe and North America caused by the introducedOphiostoma ulmibegan in the early 1900s but declined unexpectedly in Europe from the 1930s onwards after killing 30–40% of the elms. Later a second pandemic caused by the much more aggressiveOphiostoma novo‐ulmispread across the same areas and by 1990 most of Britain'sc. 30 million mature elms had died. During the second pandemic,O. novo‐ulmiacquired debilitating viruses and changed from being largely clonal to highly heterogeneous through horizontal transfer of novel, sometimes deleterious, genes fromO. ulmi. In the post‐epidemic period a new disease dynamic has emerged, with millions of small recruitment elms repeatedly attacked by sequential cycles of disease. This study investigated the possibility of pathogenic attenuation inO. novo‐ulmias the epidemic has progressed. Isolates collected from the three original outbreak areas in Britain in 1982–3 (early post‐epidemic period) and 2011 (advanced post‐epidemic) were compared for two fitness components: pathogenic aggressiveness andin vitrogrowth rate. Mean aggressiveness of the 2011 isolates to clonal English elm (Ulmus procera) proved not to differ significantly from that of the 1982 isolates, either overall or within each outbreak area. Similarly, the mean growth rates of the 1982 and 2011 samples showed no differences. The implications of these findings are discussed in relation to the future of the currentDEDpandemic in Europe, the decline of the first pandemic, and the difficulty of drawing parallels between different tree–pathogen systems.
Classical conditioning in oddball paradigm: A comparison between aversive and name conditioning
Authors: Pavlov Yuri G. , Kotchoubey Boris 
Published: March 2019

AbstractThe nature of cortical plasticity in learning is one of the most intriguing questions of modern cognitive neuroscience. Classical conditioning (as a typical case of associative learning) and electroencephalography together provide a good framework for expanding our knowledge about fast learning‐related cortical changes. In our experiment, we employed a novel paradigm in which classical conditioning was combined with passive oddball. Nineteen subjects participated in the first experiment (aversive conditioning with painful shock as unconditioned stimulus (US) and neutral tones as conditioned stimulus (CS)), and 22 subjects in the second experiment (with a subject’s own name as US). We used event‐related potentials (ERPs) and time‐frequency analyses to explore the CS‐US interaction. We found a learning‐induced increment of P3a in the first experiment and the late positive potential (LPP) in both experiments. These effects may be related to increased attentional and emotional significance of conditioned stimuli. We showed that the LPP and P3a effects, earlier found only in visual paradigms, generalize to the auditory sensory system. We also observed suppression of the low beta activity to CS+ in aversive conditioning over the hemisphere contralateral to expected electrical shocks, presumably indicating preparation of the somatosensory system to the expected nociceptive US.