bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multilocus phylogeny of the Crocidura poensis species complex (Mammalia, Eulipotyphla): Influences of the palaeoclimate on its diversification and evolution
Authors: Nicolas Violaine , Jacquet François , Hutterer Rainer , Konečný Adam , Kan Kouassi Stephane , Durnez Lies , Lalis Aude , Colyn Marc , Denys Christiane 
Published: March 2019

AbstractAimThis study aims to reconstruct the evolutionary history of the Crocidura poensis species complex and to identify factors driving diversification within it. We tested whether: (a) there is a pattern of allopatric differentiation coincident with the location of hypothesized Pleistocene forest refugia, (b) sister taxa are separated by broad rivers, (c) sister taxa occupy adjacent but distinct habitat.LocationSub‐Saharan African forests and adjacent savanna.TaxonShrews.MethodsAnalyses were based on 247 specimens collected from across the distribution of the species complex. We reconstructed the phylogeny (Bayesian and maximum likelihood methods) and assessed historical biogeography of this taxonomic group using a combination of mitochondrial and nuclear markers. We mapped the genetic diversity and estimated the divergence times by a relaxed clock model. Informed by multilocus species delimitation methods, we discussed possible taxonomic implications.ResultsThis complex is composed of nine major genetic lineages (proposed species). The earliest split within this complex occurred after 2.0–2.4 Ma, which corresponds to a period of increased aridity and/or extreme environmental variability. Most other divergence events occurred after the Early‐Middle Pleistocene Transition (1.2–0.8 Ma). Divergent selection across ecological gradients could explain diversification within the West African lineage. In Central Africa, the observed phylogeographic pattern fits the Pleistocene refuge hypothesis and supports the existence of multiple small rather than a few large forest refugia during glacial maxima. Large rivers, like the Congo and Sanaga Rivers, are important barriers to gene flow for several lineages but probably were not the primary cause of differentiation.Main conclusionsBoth geographic isolation in distinct forest refugia and divergent selection along ecological gradients could explain Pleistocene diversification within this complex.
Tetraspanin 1 as a mediator of fibrosis inhibits EMT process and Smad2/3 and beta‐catenin pathway in human pulmonary fibrosis
Authors: Liu Gang , Wang Yahong , Yang Lawei , Zou Baoan , Gao Shenglan , Song Zeqing , Lin Ziying 
Published: March 2019

AbstractTetraspanin 1(TSPAN1) as a clinically relevant gene target in cancer has been studied, but there is no direct in vivo or vitro evidence for pulmonary fibrosis (PF). Using reanalysing Gene Expression Omnibus data, here, we show for the first time that TSPAN1 was markedly down‐regulated in lung tissue of patient with idiopathic PF (IPF) and verified the reduced protein expression of TSPAN1 in lung tissue samples of patient with IPF and bleomycin‐induced PF mice. The expression of TSPAN1 was decreased and associated with transforming growth factor‐β1 (TGF‐β1)‐induced molecular characteristics of epithelial‐to‐mesenchymal transition (EMT) in alveolar epithelial cells (AECs). Silencing TSPAN1 promoted cell migration, and the expression of alpha‐smooth muscle actin, vimentin and E‐cadherin in AECs with TGF‐β1 treatment, while exogenous TSPAN1 has the converse effects. Moreover, silencing TSPAN1 promotes the phosphorylation of Smad2/3 and stabilizes beta‐catenin protein, however, overexpressed TSPAN1 impeded TGF‐β1‐induced activation of Smad2/3 and beta‐catenin pathway in AECs. Together, our study implicates TSPAN1 as a key regulator in the process of EMT in AECs of IPF.
Modeling Response Styles in Cross‐Country Self‐Reports: An Application of a Multilevel Multidimensional Nominal Response Model
Authors: Ju Unhee , Falk Carl F. 
Published: March 2019

AbstractWe examined the feasibility and results of a multilevel multidimensional nominal response model (ML‐MNRM) for measuring both substantive constructs and extreme response style (ERS) across countries. The ML‐MNRM considers within‐country clustering while allowing overall item slopes to vary across items and examination of whether certain items were more prone to ERS. We applied this model to survey items from TALIS 2013. Results indicated that self‐efficacy items were more likely to trigger ERS compared to need for professional development, and the between‐country relationships among constructs can change due to ERS. Simulations assessed the estimation approach and found adequate recovery of model parameters and factor scores. We stress the importance of additional validity studies to improve the cross‐cultural comparability of substantive constructs.
The suppressive role of HYAL1 and HYAL2 in the metastasis of colorectal cancer
Authors: Jin Zhiming , Zhang Guoliang , Liu Yiwen , He Yiqing , Yang Cuixia , Du Yan , Gao Feng 
Published: March 2019

AbstractBackgroundHyaluronidases (HAases), enzymes that degrade hyaluronan, have been widely investigated in cancer biology. However, whether HAases serve as tumor promoters or suppressors has been controversial in different cancers, and the exact role of HAases in colorectal cancer (CRC) has not been elucidated.MethodsThe expression levels of HYAL1, HYAL2, and HYAL3 in cancer and corresponding normal tissues from CRC patients were examined via immunohistochemistry. Then the correlation between HAases levels and pathological characteristics of CRC patients was analyzed. To verify the clinical data, HYAL1 and HYAL2 were downregulated or overexpressed in colon cancer cells LOVO and HCT116 to observe their influences on cell invasion and migration. For the mechanism study, we investigated the effects of HYAL1 and HYAL2 on the expression of matrix metalloproteases (MMPs)/tissue inhibitor of metalloproteases (TIMPs) and distribution of F‐actin.ResultsAll the three HAases were abnormally elevated in cancer tissues. Interestingly, HYAL1 and HYAL2, but not HYAL3, were negatively correlated with lymphatic metastasis and TNM stage. When HYAL1 and HYAL2 were knocked down, the invasion and migration abilities of colon cancer cells were accelerated, whereas overexpression of HYAL1 and HYAL2 had the opposite effects. In addition, colon cancer cells with HYAL1 and HYAL2 downregulation showed increased levels of MMP2 and MMP9, decreased levels of TIMP1 and TIMP2, and more intense F‐actin stress fibers.ConclusionsOur study suggests that HYAL1 and HYAL2 suppress CRC metastasis through regulating MMPs/TIMPs balance and rearranging F‐actin distribution, further inhibiting invasion and migration of cancer cells.
Paediatric asylum seekers in Western Australia: Identification of adversity and complex needs through comprehensive refugee health assessment
Authors: Hanes Gemma , Chee Jessica , Mutch Raewyn , Cherian Sarah 
Published: March 2019

AimAsylum seekers (ASs) report high rates of trauma and difficulty accessing health and educational services. This study aims to ascertain the needs of paediatric ASs managed by the tertiary Western Australian paediatric Refugee Health Service (RHS), including demographic features, the range of health and psychosocial issues and ongoing management challenges.MethodsAn audit of multidisciplinary RHS assessments, health records and hospital admissions for new AS patients (<16 years) between July 2012 and June 2016 was undertaken.ResultsRecords for 110 ASs were reviewed (mean age 6 years, standard deviation 4.72 years). Multiple issues (medical, psychological, developmental, educational) were identified after the first tertiary assessment (median 4, interquartile range (IQR) 3–6) compared to referral sources (median 1, IQR 0–2, P < 0.001). The median number of issues per child at audit completion was 6 (IQR 4–7, P < 0.001). Multiple refugee adverse childhood experiences were identified, with all experiencing >3 (median 4, IQR 4–5). Most had detention experience (107/110, 97.2%), family separation (91/108, 84%) and interrupted education (41/46, 89.2%). The median duration of detention was 7 months (IQR 3–12.5 months) at time of initial review across multiple sites (median 2, IQR 1–3 locations). High rates of hospital interaction were evident, with 45.4% requiring hospital admission and 36% presenting to the emergency department. The median number of outpatient appointments attended per child was 5 (IQR 2–8). Parental and child mental health concerns were identified in 53.6 and 46.3%, respectively.ConclusionsPaediatric ASs have complex trauma backgrounds with exposure to multiple adverse events within disrupted family units. The majority of Western Australian ASs assessed demonstrated negative health or education sequelae compounded by detention not previously identified prior to comprehensive paediatric review. Our data support the urgent removal of ASs from held detention. Ongoing holistic assessment and management engaging multidisciplinary trauma‐informed paediatric refugee services to optimise health and well‐being is recommended.
Demystifying dominant species
Authors: Avolio Meghan L. , Forrestel Elisabeth J. , Chang Cynthia C. , La Pierre Kimberly J. , Burghardt Karin T. , Smith Melinda D. 
Published: March 2019

SummaryThe pattern of a few abundant species and many rarer species is a defining characteristic of communities worldwide. These abundant species are often referred to as dominant species. Yet, despite their importance, the term dominant species is poorly defined and often used to convey different information by different authors. Based on a review of historical and contemporary definitions we develop a synthetic definition of dominant species. This definition incorporates the relative local abundance of a species, its ubiquity across the landscape, and its impact on community and ecosystem properties. A meta‐analysis of removal studies shows that the loss of species identified as dominant by authors can significantly impact ecosystem functioning and community structure. We recommend two metrics that can be used jointly to identify dominant species in a given community and provide a roadmap for future avenues of research on dominant species. In our review, we make the case that the identity and effects of dominant species on their environments are key to linking patterns of diversity to ecosystem function, including predicting impacts of species loss and other aspects of global change on ecosystems.
Malignant transformation of neurocutaneous melanosis (NCM) following immunosuppression
Authors: Schaff Lauren R. , Marghoob Ashfaq , Rosenblum Marc K. , Meyer Rina , Khakoo Yasmin 
Published: March 2019

AbstractNeurocutaneous melanosis (NCM) is the condition of abnormal melanocyte deposition in the leptomeninges and brain parenchyma. Associated with congenital melanocytic nevi, NCM can result in neurologic deficits, hydrocephalus, and rarely, malignant transformation of cells. We present the case of a 16‐year‐old boy with NCM who developed malignant leptomeningeal melanoma following immunosuppression with a TNFα inhibitor. To our knowledge, this is the first reported case of a patient with known NCM undergoing malignant transformation after anti‐TNF therapy for inflammatory bowel disease.
The past, present and future of pulmonary rehabilitation
Authors: Troosters Thierry , Blondeel Astrid , Janssens Wim , Demeyer Heleen 
Published: March 2019

ABSTRACTAbundant evidence supports the use of pulmonary rehabilitation as a treatment for stable and exacerbated chronic obstructive pulmonary disease. Several questions around the science base of rehabilitation in other patient groups as well as the role of rehabilitation as a component in other comprehensive care trajectories remain to be addressed. The impact of a rehabilitation programme could also perhaps be enhanced if clear guidance would be available on how to individualize the components of a rehabilitation programme in individual patients. The rehabilitation community, in an attempt to increase access to programmes, has developed less rigorous interventions. These may serve specific patients (e.g. less severe patients or may be used as a maintenance programme), but in order to have conceptual clarity they should not be called substitutes for rehabilitation if they do not meet the definition of rehabilitation. Reaching clarity on the best format for maintenance programmes in order to achieve long‐lasting health benefits for patients is another challenge. Furthermore, as many patients as possible should be referred to adequate rehabilitation programmes within their reach with fair reimbursement. Programmes should take into account the burden of the disease of a patient, the required components to tackle the problems, adequate assessment to document the outcome and the patient's preference. In summary, pulmonary rehabilitation is one of the most potent evidence‐based therapies for patients with respiratory diseases. Researchers should continue to fine tune the interventions, get clarity on terminology as well as the ultimate outcomes for rehabilitation to ensure sustainable health effects.
Conflict and Contraception in Colombia
Authors: Svallfors Signe , Billingsley Sunnee 
Published: March 2019

AbstractThis study explores how armed conflict relates to contraceptive use in Colombia, combining data from the Uppsala Conflict Data Program and Demographic and Health Surveys 1990–2016. Our study is the first systematic effort to investigate whether and how violent conflict influences women's contraceptive use, using nationally representative data across all stages of women's reproductive careers. With fixed effects linear probability models, we adjust for location‐specific cultural, social, and economic differences. The results show that although modern contraceptive use increased over time, it declined according to conflict intensity across location and time. We find no evidence that this relationship varied across socioeconomic groups. Increased fertility demand appears to explain a small portion of this relationship, potentially reflecting uncertainty about losing a partner, but conflict may also result in lack of access to contraceptive goods and services.
Efficacy, immunogenicity and safety of a recombinant tetravalent dengue vaccine (CYD-TDV) in children aged 2–17 years: systematic review and meta-analysis
Authors: Rosa Bruno Rodrigues , Cunha Antonio José Ledo Alves da , Medronho Roberto de Andrade 
Published: March 2019

BackgroundRandomised controlled trials have evaluated the recombinant tetravalent dengue vaccine (CYD-TDV). However, individual results may have little power to identify differences among the populations studied.ObjectiveTo evaluate efficacy, immunogenicity and safety of CYD-TDV in the prevention of dengue in children aged 2–17 years.DesignSystematic review and meta-analysis.Data sourcesMEDLINE (from 1950 to 5 December 2018), EMBASE (from 1947 to 5 December 2018) and Cochrane (from 1993 to 5 December 2018).Eligibility criteria of studiesRandomised trials comparing efficacy, immunogenicity and safety of CYD-TDV with placebo or other vaccines for preventing dengue cases in children aged 2–17 years.Outcome measuresEfficacy, immunogenicity and safety of CYD-TDV.Study appraisal and methodsCalculations were made of relative risk (RR) and mean difference (MD) for dichotomous and continuous outcomes, respectively. All estimates were calculated considering a 95% CI estimate. A p<0.05 was considered statistically significant.ResultsNine studies involving 34 248 participants were included. The overall efficacy of CYD-TDV was 60% (RR 0.40 (0.30 to 0.54)). Serotype-specific efficacy of the vaccine was 51% for dengue virus type-1 (DENV-1) (RR 0.49 (0.39 to 0.63)); 34% for DENV-2 (RR 0.66 (0.50 to 0.86)); 75% for DENV-3 (RR 0.25 (0.18 to 0.35)) and 77% for DENV-4 (RR 0.23 (0.15 to 0.34)). Overall immunogenicity (MD) of CYD-TDV was 225.13 (190.34 to 259.93). Serotype-specific immunogenicity was: DENV-1: 176.59 (123.36 to 229.83); DENV-2: 294.21 (181.98 to 406.45); DENV-3: 258.78 (146.72 to 370.84) and DENV-4: 189.35 (141.11 to 237.59). The most common adverse events were headache and pain at the injection site.LimitationsThe main limitation of this study was unclear or incomplete data.Conclusions and implications of key findingsCYD-TDV is considered safe and able to partially protect children and adolescents against four serotypes of DENV for a 1-year period. Despite this, research should prioritise improvements in vaccine efficacy, thus proving higher long-term protection against all virus serotypes.PROSPERO registration numberCRD42016043628.
Acute kidney injury and 1-year mortality after colorectal cancer surgery: a population-based cohort study
Authors: Slagelse Charlotte , Gammelager Henrik , Iversen Lene Hjerrild , Sørensen Henrik Toft , Christiansen Christian F 
Published: March 2019

ObjectivesAcute kidney injury (AKI) is a frequent postoperative complication, but the mortality impact within different postoperative time frames and severities of AKI are poorly understood. We examined the occurrence of postoperative AKI among colorectal cancer (CRC) surgery patients and the impact of AKI on mortality during 1 year after surgery.DesignObservational cohort study. We defined the exposure, AKI, as a 50% increase in plasma creatinine or initiation of renal replacement therapy within 7 days after surgery or an absolute increase in creatinine of 26 µmol/L within 48 hours.SettingPopulation-based Danish medical databases.ParticipantsA total of 6580 patients undergoing CRC surgery in Northern Denmark during 2005–2011 were included from the Danish Colorectal Cancer Group database.Outcomes measureOccurrence of AKI and 8–30, 31–90 and 91–365 days mortality in patient with or without AKI.ResultsAKI occurred in 1337 patients (20.3%) of the 6580 patients who underwent CRC surgery. Among patients with AKI, 8–30, 31–90 and 91–365 days mortality rates were 10.1% (95% CI 8.6% to 11.9%), 7.8% (95% CI 6.4% to 9.5%) and 12.0% (95% CI 10.3% to 14.2%), respectively. Compared with patients without AKI, AKI was associated with increased 8–30 days mortality (adjusted HR (aHR)=4.01,95% CI 3.11 to 5.17) and 31–90 days mortality (aHR 2.08,95% CI 1.60 to 2.69), while 91–365 days aHR was 1.12 (95% CI 0.89 to 1.41). We observed no major differences in stratified analyses.ConclusionsAKI after surgery for CRC is a frequent postoperative complication associated with a substantially increased 90-day mortality. AKI should be considered a potential target for reducing 90-day mortality.
Use of out-of-hours primary care in affluent and deprived neighbourhoods during reforms in long-term care: an observational study from 2013 to 2016
Authors: Jansen Tessa , Verheij Robert A , Schellevis Francois G , Kunst Anton E 
Published: March 2019

ObjectivesMajor long-term care (LTC) reforms in the Netherlands in 2015 may specifically have disadvantaged socioeconomically deprived groups to acquire LTC, possibly impacting the use of acute care. We aimed to demonstrate whether LTC reforms coincided with changes in the use of out-of-hours (OOH) primary care services (PCSs), and to compare changes between deprived versus affluent neighbourhoods.DesignEcological observational retrospective study using routinely recorded electronic health records data from 2013 to 2016 and population registry data.SettingData from 15 OOH PCSs participating in the Nivel Primary Care Database (covering approximately 6.5 million inhabitants) in the Netherlands. PCS utilisation data on neighbourhood level were matched with sociodemographic characteristics, including neighbourhood socioeconomic status (SES).ParticipantsElectronic health records from 6 120 384 OOH PCS contacts in 2013–2016, aggregated to neighbourhood level.Outcome measures and analysesNumber of contacts per 1000 inhabitants/year (total, high/low-urgency, night/evening-weekend-holidays, telephone consultations/consultations/home visits).Multilevel linear regression models included neighbourhood (first level), nested within PCS catchment area (second level), to account for between-PCS variation, adjusted for neighbourhood characteristics (for instance: % men/women). Difference-in-difference in time-trends according to neighbourhood SES was assessed with addition of an interaction term to the analysis (year×neighbourhood SES).ResultsBetween 2013 and 2016, overall OOH PCS use increased by 6%. Significant increases were observed for high-urgency contacts and contacts during the night. The largest change was observed for the most deprived neighbourhoods (10% compared with 4%–6% in the other neighbourhoods; difference not statistically significant). The increasing trend in OOH PCS use developed practically similar for deprived and affluent neighbourhoods. A a stable gradient reflected more OOH PCS use for each lower stratum of SES.ConclusionsLTC reforms coincided with an overall increase in OOH PCS use, with nearly similar trends for deprived and affluent neighbourhoods. The results suggest a generalised spill over to OOH PCS following LTC reforms.
Are ‘dual users’ who smoke and use e-cigarettes interested in using varenicline to stop smoking altogether, and can they benefit from it? A cohort study of UK vapers
Authors: Hajek Peter , Peerbux Sarrah , Phillips-Waller Anna , Smith Charlotte , Pittaccio Kate , Przulj Dunja 
Published: March 2019

ObjectivesSmokers who use e-cigarettes (EC) do so mostly to stop smoking, but many continue to use both products. It is not known whether these ‘dual users’ are interested in stop-smoking medications and whether they can benefit from them.Setting, participants and measuresDual users were recruited over social media and posted study questionnaire and saliva kits at baseline, 3 and 6 months. Those interested in varenicline were posted the medication and received weekly calls over the first 6 weeks, followed by three calls at fortnightly intervals.ResultsOf 204 participants, 124 (61%, CI=54% to 68%) expressed interest in receiving varenicline and 80 (39%, CI=32% to 45%) started varenicline (varenicline users, VU). VU were more dependent smokers (F=6.2, p=0.01) with higher cigarette consumption (F=8.7, p<0.01) who were using stronger nicotine e-liquids (F=13.9, p<0.001) than dual users not opting for varenicline (varenicline non-users, VN). In terms of abstinence for at least 3 months at the 6-month follow-up, VU were more likely than VN to report abstinence from smoking (17.5% vs 4.8%, p=0.006, RR=3.6, CI:1.4 to 9.0), vaping (12.5% vs 1.6%, p=0.007, RR=7.8, CI:1.7 to 34.5) and both smoking and vaping (8.8% vs 0.8%, p=0.02, RR=10.9, CI:1.4 to 86.6). The differences were significant across sensitivity analyses (RRs=4.9 to 14.0; p=0.02 to p<0.001 at 3 months; RRs=3.0 to 14.0; p=0.01 to p<0.001 at 6 months). VU reported a greater reduction in enjoyment of vaping by the end of the varenicline use period (F=4.1, p=0.04) and recorded a significantly greater reduction in nicotine intake than VN at 3 months (F=13.9, p<0.001) and 6 months (F=26.5, p<0.001).ConclusionVarenicline offered to dual users is likely to promote successful abstinence from both smoking and vaping, although a randomised trial is needed to confirm this. Among dual users who want to stop smoking, there seems to be a high level of interest in smoking-cessation treatments.