bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surfactant‐assisted‐water‐exposed versus surfactant‐aqueous‐solution‐exposed electrospinning of novel super hydrophilic Polycaprolactone‐based fibers: Cell culture studies
Authors: Zargarian Seyed Shahrooz , Haddadi‐Asl Vahid , Azarnia Mojdeh , Kafrashian Zahra , Seyedjafari Ehsan 
Published: January 2019

AbstractBlend electrospun scaffolds composed of Polycaprolactone and Pluronic are suitable for bone tissue engineering due to their excellent biocompatibility and hydrophilicity. However, exceeding from certain amounts of Pluronic, surface enrichment of this polymer leads to segregation of Pluronic chains within the fiber, endangering the integrity and mechanical properties of the scaffold. In this article, a novel method of blend electrospinning has been employed using a parallel water supply, positioning the Pluronic chains on the surface, thus enhancing the miscibility within the fibers. Water uptake test revealed the super hydrophilicity of obtained scaffolds. Atr‐FTIR and X‐ray photoelectron spectroscopy verified a higher percentage of Pluronics on the surface in comparison with conventional blend electrospinning. Tensile test demonstrated improved mechanical properties of the modified scaffolds. The results of cytocompatibility tests have also revealed enhanced viability of cells on these scaffolds confirming their great promise for clinical applications. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1204–1212, 2019.
Potential use of silkworm gut fiber braids as scaffolds for tendon and ligament tissue engineering
Authors: Pagán Ana , Aznar‐Cervantes Salvador D. , Pérez‐Rigueiro José , Meseguer‐Olmo Luis , Cenis Jose L. 
Published: January 2019

AbstractTendon and ligament tissue engineering require scaffolds for the treatment of various conditions in the medical field. These must meet requirements such as high tensile strength, biocompatibility, fast and stable repair and a rate of degradation that allows the repair of the damaged tissue. In this work, we propose the use of silkworm gut fiber braids as materials to temporarily replace and repair this type of tissues. The mechanical characterization of the braids made with different number of silk gut fibers is provided, as well as a descriptive analysis of the proliferation and adhesion of cultures of adult human mesenchymal stem cells from bone marrow and fibroblasts (L929) on the braids. As expected, the breaking force increases linearly in the scaffold with the number of fibers, thus being a parameter adaptable to the specific requirements of the tissue to repair and the animal model of study. On the other hand, in all of the cases studied, the values obtained for the elastic modulus of the hydrated fibers were in the range of the ones reported for various human tendons and ligaments. Moreover, the scaffold demonstrated excellent biocompatibility in vitro, allowing the adhesion and proliferation, in the same culture conditions, of the two cell types studied, therefore posing as an ideal candidate to be employed in future in vivo studies that allow elucidating its behavior in the articular environment or extra‐articular tendinous areas. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res B Part B: 2019. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2209–2215, 2019.
Electrodialytic processes in solid matrices. New insights into battery recycling. A review
Authors: Villen‐Guzman Maria , Arhoun Brahim , Vereda‐Alonso Carlos , Gomez‐Lahoz Cesar , Rodriguez‐Maroto Jose M , Paz‐Garcia Juan M 
Published: January 2019

AbstractElectrodialytic remediation has been widely applied to the recovery of different contaminants from numerous solid matrices, solving emerging issues of environmental concern. Results and conclusions reported in studies about real contaminated matrices are summarized in this work. The influence of pH value on the treatment effectiveness has been widely proved, highlighting the phenomenon of ‘water splitting’ in the membrane surface. This dissociation of water molecules is related to the ‘limiting current’ which it is desirable to exceed at the anion exchange membrane in order to produce the entering of protons toward the solid matrix. Other important parameters for the optimization of the technique, such as the current density and the liquid/solid ratio, are also discussed through the revision of studies using real solid matrices.This work also focuses on the pioneer proposal of electrokinetic technologies for the recycling of lithium ion batteries, considering the relevance of waste properties in the design and optimization of the technique. From a thorough literature revision, it could be concluded that further experimental results are needed to allow an optimal application of the technique to the rising problem of residues from batteries. The main aim of this work is to take the first steps in the recovery of valuable metals such as Li and Co from spent batteries, incorporating principles of green chemistry. © 2019 Society of Chemical Industry
Factors affecting breast cancer patients' need for genetic risk information: From information insufficiency to information need
Authors: Hong Soo Jung , Biesecker Barbara , Ivanovich Jennifer , Goodman Melody , Kaphingst Kimberly A. 
Published: January 2019

AbstractInformation‐seeking models typically focus on information‐seeking behaviors based on individuals' interest in information, because their current level is perceived to be insufficient. In the context of genetic risk information (GRI), however, information insufficiency is difficult to measure and thus can limit understanding of information behavior in the context of GRI. We propose that an individual's need for information might be a more direct and conceptually clearer alternative to predicting their information‐seeking behavior. To test this hypothesis, this study investigates the extent to which previously identified factors affecting interest in GRI are also predictors of need for GRI among women diagnosed with breast cancer at the age of 40 or younger (N = 1,069). As hypothesized, there was a positive association between interest in and need for GRI. Furthermore, hypothesized factors of numeracy, information orientation, and genetic knowledge were significant predictors of increased interest in and need for GRI. In contrast, hypothesized factors of genetic worry and genetic causal belief predicted increased interest in GRI only, while genetic self‐efficacy predicted increased need for GRI only. As hypothesized, BRCA status significantly moderated associations between informational norm and both interest in and need for GRI. Collectively, the findings support inclusion of need for GRI in theoretical information‐seeking models in the context of genomic risk.
Cardiac disease in mucopolysaccharidosis type III
Authors: Nijmeijer Stephanie C. M. , de Bruin‐Bon Rianne H.A.C.M. , Wijburg Frits A. , Kuipers Irene M. 
Published: January 2019

AbstractMucopolysaccharidosis type III (MPS III; Sanfilippo disease) is primarily characterized by neurocognitive decline with limited somatic disease. Only few reports addressed cardiac disease (CD) in MPS III. We investigated the prevalence of CD in a relatively large cohort of patients. In this cross‐sectional study, extensive echocardiographic studies were performed in 30 MPS III patients (16 patients <18 years), all without clinical symptoms of CD. Results were compared to data from matched controls. The mean global longitudinal strain on speckle‐tracking echocardiography (STE) was impaired in both pediatric and adult patients vs controls (resp. −18.4% vs −20.7%; mean difference 2.25, 95% CI 0.61‐3.89, P = 0.009 and −16.9% vs −19.5%; mean difference 2.64, 95% CI 0.78‐4.49, P = 0.007), indicating early systolic dysfunction. Left ventricle ejection fraction (LVEF) was normal in pediatric patients and (slightly) impaired in adult patients vs controls (48.7% vs 55.8%, P = 0.002). Tissue Doppler imaging (TDI) showed significantly slower early diastolic velocities (e′) compared to controls indicative for diastolic dysfunction. Furthermore, mitral and aortic valve abnormalities were prevalent (43% and 33% of patients, respectively). Finally, 15.6% of the patients had a first‐degree atrioventricular block on electrocardiography (ECG). The impaired STE reveals early, subclinical LV dysfunction which is supported by results of TDI. In addition, mild valvular disease and ECG abnormalities are prevalent. The lowered LVEF in adult patients suggests that the LV dysfunction is progressive, and may ultimately lead to clinical myocardial disease when patients live longer due to an effective disease‐modifying treatment of which a number of options are now in clinical trials.
The multiple faces of CD5
Authors: Burgueño-Bucio Erica , Mier-Aguilar Carlos A , Soldevila Gloria 
Published: January 2019

AbstractSince its discovery, over 30 years ago, CD5 has been used as a marker to identify T cells, B1-a cells, and B cell chronic lymphocytic leukemia cells. Throughout the years, many studies have described the functional relevance of CD5 as a modulator of T and B cell receptor signaling. However, it has not been until recent years that CD5 has emerged as a functional receptor in other areas of the immune system. Here, we review some of the most important aspects of CD5 as a modulator of TCR and BCR signaling, cell survival receptor both in T and B cells during health and disease, as well as the newly discovered roles of this receptor in thymocyte selection, T cell effector differentiation, and immune tolerance. CD5 was found to promote T cell survival by protecting autoreactive T cell from activation-induced cell death, to promote de novo induction of regulatory T cells in the periphery, to modulate Th17 and Th2 differentiation, and to modulate immune responses by modulating dendritic cell functions. CD5 is overexpressed in Tregs and Bregs, which are fundamental to maintain immune homeostasis. The newly established roles of CD5 in modulating different aspects of immune responses identify this receptor as an immune checkpoint modulator, and therefore it could be used as a target for immune intervention in different pathologies such as cancer, autoimmune diseases or infections.
Microscopical observations on the regenerating tail in the tuataraSphenodon punctatusindicate a tendency to scarring, but also influence from somatic growth
Authors: Alibardi Lorenzo , Meyer‐Rochow Victor Benno 
Published: January 2019

AbstractThe process of tail regeneration in the tuatara (Sphenodon punctatus) is not entirely known. Similarity to and differences from lizard tail regenerations are indicated in the present histological and ultrastructural study. Regeneration is influenced by the animal's age and ambient temperature, but in comparison to that of lizards it is very slow and tends to produce outgrowths that do not reach the length of the original tail. Although microscopically similar to lizard blastemas, the mesenchyme rapidly gives rise to a dense connective tissue that contains few muscle bundles, nerves, and fat cells. The unsegmented cartilaginous tube forming the axial skeleton is not calcified after 5 months of regeneration, but calcification in the inner region of the cartilage, present after 10 months, increases thereafter. Amyelinic and myelinic peripheral nerves are seen within the regenerating tails of 2–3 mm in length and the spinal cord forms an ependymal tube inside a cartilaginous casing. Tissues of the original tail, like muscles, vertebrae and the adipose mass, are largely replaced by dense connective tissue that occupies most of the volume of the new tail at 5 and 10 months of regeneration. It is unknown whether the differentiation of the dense connective tissue is caused by the relatively low temperature that this species lives under or stems from a genetic predisposition toward scarring as with most other amniotes. Increases of muscle and adipose tissues seen in older regenerated tails derive from somatic growth of the new tail in the years following tail loss and not from a rapid regeneration process like that in lizards.
Deep‐ultraviolet laser ablation electrospray ionization mass spectrometry
Authors: Lawal Remilekun O. , Donnarumma Fabrizio , Murray Kermit K. 
Published: January 2019

AbstractA 193‐nm wavelength deep ultraviolet laser was used for ambient laser ablation electrospray ionization mass spectrometry of biological samples. A pulsed ArF excimer laser was used to ablate solid samples, and the resulting plume of the desorbed material merged with charged electrospray droplets to form ions that were detected with a quadrupole time‐of‐flight mass spectrometer. Solutions containing peptide and protein standards up to 66‐kDa molecular weight were deposited on a metal target, dried, and analyzed. No fragmentation was observed from peptides and proteins as well as from the more easily fragmented vitamin B12 molecule. The mass spectra contained peaks from multiply charged ions that were identical to conventional electrospray. Deep UV laser ablation of tissue allowed detection of lipids from untreated tissue. The mechanism of ionization is postulated to involve absorption of laser energy by a fraction of the analyte molecules that act as a sacrificial matrix or by residual water in the sample.
Advancing Creativity Theory and Research: A Socio‐cultural Manifesto
Authors: Glaveanu Vlad Petre , Hanchett Hanson Michael , Baer John , Barbot Baptiste , Clapp Edward P. , Corazza Giovanni Emanuele , Hennessey Beth , Kaufman James C. , Lebuda Izabela , Lubart Todd , Montuori Alfonso , Ness Ingunn J. , Plucker Jonathan , Reiter‐Palmon Roni , Sierra Zayda , Simonton Dean Keith , Neves‐Pereira Monica Souza , Sternberg Robert J. 
Published: January 2019

AbstractThis manifesto, discussed by 20 scholars, representing diverse lines of creativity research, marks a conceptual shift within the field. Socio‐cultural approaches have made substantial contributions to the concept of creativity over recent decades and today can provide a set of propositions to guide our understanding of past research and to generate new directions of inquiry and practice. These propositions are urgently needed in response to the transition from the Information Society to the Post‐Information Society. Through the propositions outlined here, we aim to build common ground and invite the community of creativity researchers and practitioners to reflect up, study, and cultivate creativity as a socio‐cultural phenomenon.
Effects of tibiofemoral compression on ACL forces and knee kinematics under combined knee loads
Authors: Markolf Keith , Yamaguchi Kent , Matthew Justin , McAllister David 
Published: January 2019

ABSTRACTInjuries to the anterior cruciate ligament (ACL) can occur during landing from a jump or changing direction during a cutting maneuver. In these instances, the knee is subjected to combined forces and moments as it flexes under tibiofemoral compression force (TCF). We hypothesized that TCF would increase ACL forces and tibiofemoral motions under isolated and combined modes of loading relevant to knee injury. ACL force and knee kinematics were recorded in human cadaveric specimens during knee flexion from 0° to 50° under the following test conditions (alone and in combination): 2 N‐m internal tibial torque (IT), 5 N‐m valgus moment (VM), and 45N anterior tibial force (AF). Knees were tested with 25N (baseline), 250N, and 500N TCF. ACL force increased with knee flexion during all tests. As the knee was flexed, VM produced a coupled internal tibial rotation, and IT produced a coupled valgus rotation. ACL forces with IT + VM were significantly higher than with IT alone (beyond 10° flexion) or VM alone (at all flexion angles). Increasing the level of TCF above baseline did not significantly change valgus or tibial rotations for any loading condition, but did significantly increase anterior tibial translation (ATT) at all flexion angles and ACL force at flexion angles beyond 5° to 15°. Addition of AF to tests with IT + VM significantly increased ATT and ACL force without significantly altering internal and valgus rotations. The mechanism of high ACL force generation from increased TCF was related to ATT and not internal or valgus rotations of the tibia. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res
Creativity as a source of differentiation in urban tourism: The case of Torino city
Authors: Adamo Giuseppe Emanuele , Ferrari Sonia , Gilli Monica 
Published: January 2019

AbstractCities tourist competition focus on the revalorization of endowed resources, such as historical and cultural heritage, and on created resources, such as flagship projects and iconic architectural buildings. This paper analyzes how creativity (creative planning and thinking) supports both approaches by developing a conceptual model that categorizes and explores four main urban competition strategies: exploitation, rediscovery, adaptability, and development/co‐creation. Creativity drives competition and differentiation taking into account the tourist offer, the role of the private and the public players, the communication, and the consumption. Finally, it presents a case study of Torino city, identifying the creative tourism activities implemented.
Association of the TBK1 mutation p.Ile334Thr with frontotemporal dementia and literature review
Authors: Yu Huiling , Yu Wenbo , Luo Su‐shan , Yang Yu‐Jie , Liu Feng‐Tao , Zhang Yue , Chen Yan , Sun Yi‐min , Wu Jian‐jun 
Published: January 2019

AbstractBackgroundThe mutation of TANK‐binding kinase 1 (TBK1) gene has been regarded as a causative gene of frontotemporal dementia (FTD)‐amyotrophic lateral sclerosis (ALS) spectrum disease in recent years. So far, more than 70 TBK1 variants have been identified in patients with FTD‐ALS spectrum.MethodsWe reported a Chinese FTD patient carrying TBK1 p.Ile334Thr variant detected by target sequencing and Sanger sequencing. The patient's clinical materials were collected. The transcription and translation levels of TBK1 mutant were investigated in fibroblast by qPCR and western blot. The effects of TBK1 mutant in inflammation pathway and autophagy were detected by luciferase reporter assay and GST pull‐down assay.ResultsThe patient was diagnosed as behavioral variant FTD (bvFTD) and displayed progressively severe cognitive impairment especially in executive function. A pattern of frontotemporal atrophy and hypometabolism was shown through MRI and PET‐CT. In vitro functional experiments of TBK1 p.Ile334Thr variant demonstrated reduced transcription and translation levels, decreased kinase activity but maintenance of interaction with optineurin. The variant was classified as likely pathogenic according to American College of Medical Genetics and Genomics guideline.ConclusionWe proposed the TBK1 mutation p.Ile334Thr as a likely pathogenic variant in bvFTD which also expanded the clinical spectrum of this variant. It can partially abrogate TBK1 functions and be responsible for FTD‐ALS spectrum diseases through neuroinflammatory pathway.
Reconstruction of complex soft tissue defects including tendons with anterolateral thigh flap extended to fascia lata: Long term recovery and functional outcomes
Authors: di Summa Pietro G. , Sapino Gianluca , Cherubino Mario , De Santis Giorgio , Durand Sebastien , Zaugg Patrice , Bauquis Olivier , Raffoul Wassim 
Published: January 2019

BackgroundWe evaluated composite anterolateral thigh (ALT) flaps including vascularized fascia lata (FL), for stable soft tissue coverage and tendons restoration at various joint levels in a one‐stage procedure.MethodsWe performed a retrospective investigation including 21 “functional” ALT flaps between November 2006 and December 2016. In all patients included, FL was shaped to anatomical reproduce a tendon structure. Functional analysis included range of motion and force assessment. Functional scores were established according to Chen classification, DASH, and LEFS score. Defects resulted from tumor excision, trauma, burn, or infection‐debridement and were distributed in four main anatomical districts: knee (seven cases), ankle (six cases), forearm‐elbow (four cases), and hand‐wrist level (three cases).ResultsNineteen flaps were raised as free flaps, while two as distally‐based propeller flaps. Average follow‐up was 38 months. Major complication requiring the harvest of a second flap was seen in two patients, whereas three flaps presented superficial necrosis and was treated in an outpatient regimen. We observed 81% of total ROM recovery compared to contralateral sides with 89% recovered articular stability. Best articulation outcomes were present in elbow reconstruction, while ankle reconstructions showed less articularity. Hospital stay was significantly reduced in hand and wrist functional reconstruction when compared with reconstruction at the ankle level (P < 0.05).ConclusionThe ALT flap extended to vascularized FL provides a particularly effective and resistant tissue that can be folded to reconstruct and support tendinous structures. This can restore functional and structural integrity after complex defects in a single stage procedure.
Frequency‐specific functional connectivity related to the rehabilitation task of stroke patients
Authors: Lu Kuan , Xu Gongcheng , Li Wenhao , Huo Congcong , Liu Qianying , Lv Zeping , Wang Yonghui , Li Zengyong , Fan Yubo 
Published: January 2019

PurposesStroke survivors suffering from deficits in motor control typically show persistent motor symptoms and limited functional abilities, which affect their functional independence in daily life. Active rehabilitation training is commonly applied for stroke patients to recover from motor dysfunction. The global connectivity reflects the synchronization of cardiac and respiratory activities in the cerebral regions. However, the understanding of the patterns of frequency‐specific global connectivity (GC) and functional connectivity (FC) when performing active rehabilitation training is still far from comprehensive. This study was conducted to investigate the brain network patterns of stroke patients while performing a four‐limb linkage rehabilitation training using the functional near‐infrared spectroscopy (fNIRS) method.MethodsTwo groups of stroke patients (LH, left hemiplegia; RH, right hemiplegia) and one healthy group were recruited to participate in this study. The wavelet phase coherence (WPCO) method was used to calculate the frequency‐specific GC and FC of the brain network in four frequency bands: I, 0.6–2 Hz; II, 0.145–0.6 Hz; III, 0.052–0.145 Hz; and IV, 0.021–0.052 Hz.ResultsResults showed that the healthy group exhibited lower WPCO in the four frequency bands during the task state than during the resting state (P < 0.05). Interestingly, the stroke groups showed increased WPCO in the frequency band II during the task state than during the resting state (P < 0.05). Moreover, significantly lower WPCO values in the frequency bands III (P < 0.05) were found during task state in the RH and LH groups compared with the healthy group. The RH group showed increased WPCO values in the frequency band II during the task state compared with the healthy group (P < 0.05). In addition, the RH group showed increased WPCO in the frequency bands I (P < 0.05) and II (P < 0.05) than the LH group. Notably, the rehabilitation task did not induce significant changes in stroke groups in the frequency band IV, which implied the neurogenic activity.ConclusionsThe reductions in FC suggested that the brain impairments caused a disturbed neurovascular coupling regulation in stroke patients. Results in frequency band IV suggested that the limb movement rehabilitation task intrinsically may not produce remarkable effect on the neurogenic activity of stroke patients. Significant differences in WPCO between the LH and RH groups suggested that the rehabilitation task should be specifically designed for individual rehabilitation. The frequency‐specific FC methods based on WPCO would provide a potential approach to quantitatively assess the effect of rehabilitation tasks.