bluuebunny/crossref_metadata_2025_split huggingface dataset:
Population structure and linkage disequilibrium in a large collection of Fusarium oxysporum strains analysed through iPBS markers
Authors: Ates Duygu , Altinok Hacer H. , Ozkuru Esin , Ferik Filiz , Erdogmus Semih , Can Canan , Tanyolac Muhammed Bahattin 
Published: August 2019

AbstractIn the current study, 160 pathogenic strains of Fusarium oxysporum collected from tomato, eggplant and pepper were studied. Eighteen inter‐primer binding site (iPBS)‐retrotransposon primers were used, and these primers generated 205 scorable polymorphic bands. The number of polymorphic bands per primer varied between 9 and 19, with a mean of 11 bands per primer. The highest polymorphism information content (PIC) value was determined as 0.27, and the lowest was 0.05. The unweighted pair‐group method with arithmetic averages (UPGMA) dendrogram including a heat map revealed that the 160 pathogenic strains of F. oxysporum were divided into two main clusters. The first cluster mainly included F. oxysporum f. sp. capsici (FOC) and F. oxysporum f. sp. melongenae (FOMG) isolates. The second cluster mainly comprised F. oxysporum f. sp. lycopersici (FOL) and F. oxysporum f. sp. radicis lycopersici (FORL) isolates. The highest percentage of loci in significant linkage disequilibrium (LD) was detected for FOL, whereas the lowest level of LD was found for FOC, and 95.2%, 99.4%, 99.1% and 97.4% of the relative kinship estimates were less than 0.4 for FOL, FOMG, FORL and FOC, respectively. LD differences were detected among formae speciales, and LD was higher in FOL as compare to FOC species. The findings of this study confirm that iPBS‐retrotransposon markers are highly polymorphic at the intraspecific level in Fusarium spp.
Computed tomography imaging characteristics of canine nasal chondrosarcoma
Authors: Jania R. , Boudreaux B. , Langohr I. , Looper J. , Rademacher N. 
Published: August 2019

ObjectivesTo describe the CT appearance of canine nasal chondrosarcoma and compare calcification patterns with those described in humans.Materials and MethodsCT examinations of dogs with biopsy‐confirmed nasal chondrosarcoma were retrospectively reviewed and evaluated, including description of calcification patterns.ResultsMajor findings are consistent with the previously described CT appearance of canine nasal tumours. Additionally, calcification patterns described as features of human nasal chondrosarcomas were also identified.Clinical SignificanceFindings may help prioritise differential lists of destructive nasal masses seen on CT in dogs.
Usefulness of noninvasive shear wave elastography for the assessment of hepatic fibrosis in dogs with hepatic disease
Authors: Tamura Masahiro , Ohta Hiroshi , Shimbo Genya , Osuga Tatsuyuki , Sasaki Noboru , Morishita Keitaro , Kagawa Yumiko , Takiguchi Mitsuyoshi 
Published: August 2019

AbstractBackgroundTwo‐dimensional shear wave elastography (2D‐SWE) can noninvasively evaluate hepatic elastic modulus as shear wave velocity (SWV). Additionally, it may predict the presence of clinical relevant hepatic fibrosis (≥F2) in dogs with hepatic disease.ObjectivesTo investigate whether SWV measured by 2D‐SWE can differentiate between dogs with (≥F2) and without (F0‐1) clinically relevant hepatic fibrosis.AnimalsTwenty‐eight client‐owned dogs with hepatic disease and 8 normal healthy Beagle dogs were enrolled.MethodsIn this cross‐sectional prospective study, SWVs were measured using 2D‐SWE in all dogs. Hepatic fibrosis stages and necroinflammatory activity grades were histopathologically evaluated using a histological scoring scheme that was adapted from the Ishak schema used in human medicine.ResultsMedian SWVs were significantly higher in dogs with clinically relevant hepatic fibrosis (2.04 m/s; range, 1.81‐2.26 m/s) than in healthy dogs (1.51 m/s; range, 1.44‐1.66 m/s; P = .007), and dogs without clinically relevant hepatic fibrosis (1.56 m/s; range, 1.37‐1.67 m/s; P < .001). However, no significant difference was found in the SWVs between dogs without clinically relevant hepatic fibrosis and healthy dogs (P = .99). Furthermore, median SWVs were not significantly different among dogs with necroinflammatory activity, those without necroinflammatory activity, and healthy dogs (Kruskal‐Wallis test, P = .12).Conclusions and Clinical ImportanceThe 2D‐SWE may be useful for predicting the presence of hepatic fibrosis in dogs with hepatic disease.
Cognitive burden and polypharmacy in elderly Japanese patients treated with anticholinergics for an overactive bladder
Authors: Shiota Takako , Torimoto Kazumasa , Okuda Masahiro , Iwata Ryo , Kumamoto Hiromi , Miyake Makito , Hirayama Akihide , Tanaka Nobumichi , Fujimoto Kiyohide 
Published: August 2019

AbstractThis study aimed to investigate the cognitive burden and polypharmacy in elderly patients treated with anticholinergics for an overactive bladder. We conducted a retrospective study of patients with an overactive bladder receiving treatment at two hospitals in Japan (Nara Medical University Hospital and Saiseikai Nara Hospital). Prescription data were collected from the medical records of the patients registered between January 2013 and April 2014. The Anticholinergic Cognitive Burden Scale was used to estimate the severity of the anticholinergic effects on the cognition of each patient. We collected the prescription data of 584 and 246 patients from the Nara Medical University Hospital and Saiseikai Nara Hospital, respectively. The mean daily total Anticholinergic Cognitive Burden score ranged between 3 and 4 (3.59 ± 1.16 at Nara Medical University Hospital vs 3.32 ± 0.78 at Saiseikai Nara Hospital, P < 0.01). At both hospitals, the mean number of prescriptions was >5 in patients ≥75 years (5.95 ± 4.43 and 5.64 ± 3.90 at Nara Medical University Hospital and Saiseikai Nara Hospitals, respectively). Our findings suggest that 10%–20% of elderly patients (≥65 years) receiving treatment with anticholinergics for an overactive bladder are in a state of polypharmacy. The total anticholinergic cognitive burden of each patient mainly depends on the anticholinergics being used for treating the overactive bladder. Especially for elderly patients with a high risk of adverse effects, including cognitive impairment, careful attention needs to be paid during selection of drugs for treating patients with an overactive bladder.
Water potential control of turgor‐driven tracheid enlargement in Scots pine at its xeric distribution edge
Authors: Cabon Antoine , Fernández‐de‐Uña Laura , Gea‐Izquierdo Guillermo , Meinzer Frederick C. , Woodruff David R. , Martínez‐Vilalta Jordi , De Cáceres Miquel 
Published: August 2019

Summary

The extent to which water availability can be used to predict the enlargement and final dimensions of xylem conduits remains an open issue.

We reconstructed the time course of tracheid enlargement in Pinus sylvestris trees in central Spain by repeated measurements of tracheid diameter on microcores sampled weekly during a 2 yr period. We analyzed the role of water availability in these dynamics empirically through time‐series correlation analysis and mechanistically by building a model that simulates daily tracheid enlargement rate and duration based on Lockhart's equation and water potential as the sole input.

Tracheid enlargement followed a sigmoid‐like time course, which varied intra‐ and interannually. Our empirical analysis showed that final tracheid diameter was strongly related to water availability during tracheid enlargement. The mechanistic model was calibrated and successfully validated (R2 = 0.92) against the observed tracheid enlargement time course. The model was also able to reproduce the seasonal variations of tracheid enlargement rate, duration and final diameter (R2 = 0.84–0.99).

Our results support the hypothesis that tracheid enlargement and final dimensions can be modeled based on the direct effect of water potential on turgor‐driven cell expansion. We argue that such a mechanism is consistent with other reported patterns of tracheid dimension variation.
Association of hypokalemia with cortisol and ACTH levels in Cushing's disease
Authors: Fan Linling , Zhuang Yuan , Wang Yi , Liu Xinhua , Liu Dan , Xiang Boni , He Min , Zhang Zhaoyun , Li Yiming , Wang Yongfei , Zhu Xiaoming , Ye Hongying 
Published: August 2019

AbstractHypokalemia is a common feature in patients with Cushing's syndrome (CS). Whether the occurrence of hypokalemia is associated with cortisol and adrenocorticotropic hormone (ACTH) levels is still unclear. Approximately 80% of cases of endogenous CS are due to Cushing's disease (CD). The purpose of this study was to determine the association of hypokalemia with cortisol and ACTH levels in patients with CD. The retrospective study included 195 patients with CD referred to our medical center from January 2011 to December 2017. The results show that 25.64% (50/195) of the patients had hypokalemia. The 24‐h urinary free cortisol (UFC) and plasma cortisol levels were significantly higher in patients with hypokalemia than those with normokalemia (P < 0.05). Plasma ACTH levels were similar between the patients with hypokalemia and normokalemia (P > 0.05). Cortisol levels were negatively correlated with plasma potassium levels (08:00: r = −0.344 (P < 0.01), 00:00: r = −0.435 (P < 0.01); 24‐h UFC: r = −0.281 (P < 0.05)). There was no significant correlation between the plasma ACTH and potassium (08:00: r = −0.093 (P > 0.05), 00:00: r = −0.184 (P > 0.050)). Our current data suggest that cortisol level, instead of ACTH level, is correlated with plasma potassium level. A high cortisol level may be the principal cause of hypokalemia.
Risk Factors for Functional Outcomes of the Elderly with Intertrochanteric Fracture: A Retrospective Cohort Study
Authors: Ju Jia‐bao , Zhang Pei‐xun , Jiang Bao‐guo 
Published: August 2019

ObjectiveTo identify baseline factors relevant to functional outcomes and health‐related quality of life in the elderly with intertrochanteric fractures.MethodsFor the present study, 168 patients with intertrochanteric fracture who were assigned to different treatments between January 2016 and December 2017 were retrospectively selected. Hip function was assessed by Harris hip score (HHS), and health‐related quality of life was evaluated by Barthel index (BI) of activities of daily living (ADL) and EuroQol 5‐dimensions (EQ‐5D) score, respectively. Data were analyzed by t‐test, ANOVA, Pearson's correlation, χ2‐test, and multivariate linear regression.ResultsA total of 164 (97.6%) patients completed the follow‐up, with an average follow‐up time of 15.7 ± 6.9 months; 39 (23.8%) patients died during the follow‐up period and 125 (76.2%) patients were eligible for the functional analysis. HHS at final follow‐up of 125 patients was 71.8 ± 13.1, and the following were associated with hip functional recovery: age (−0.45, 95% confidence interval (CI) −0.73 to −0.18, P < 0.01), serum albumin (0.65, 95% CI 0.04 to 1.27, P < 0.05), and ADL at discharge (0.18, 95% CI 0.01 to 0.33, P < 0.05). The Barthel index at final follow up in this cohort was 80.2 ± 18.1, and multivariable linear regression analysis showed that age (−0.49, 95% CI −0.85 to −0.12; P < 0.05), ADL score at discharge (0.29, 95% CI 0.07 to 0.51; P < 0.05) and internal fixation (16.3, 95% CI 3.3 to 29.3; P < 0.05) were associated with ADL at final follow‐up. EQ‐5D at final follow‐up was 0.74 ± 0.2, with which HHS (0.012, 95% CI 0.011 to 0.013; P < 0.01) was positively associated.ConclusionWe identify several baseline factors associated with hip functional outcome, health utility, and ADL in the elderly after an intertrochanteric fracture, of which we could modify mutable factors to achieve better outcomes. These findings could help to inform treatment and functional prognosis.
Elastic Stable Titanium Flexible Intramedullary Nails Versus Plates in Treating Low Grade Comminuted Femur Shaft Fractures in Children
Authors: Luo Yi , Wang Lin , Zhao Li‐hua , Wang Yi‐cheng , Chen Meng‐jie , Wang Sun , Ma Qi‐chao 
Published: August 2019

ObjectivesTo review the experience at our center with elastic stable titanium flexible intramedullary nails (ESIN) for pediatric femoral shaft unstable fractures.MethodsFrom January 2015 to January 2017, 56 consecutive patients with femur shaft fractures were treated in our hospital. History and radiologic parameters were retrospectively reviewed. The inclusion criteria were: femur shaft fractures treated by ESIN or locking compressing plates (LCP); more than 2 years’ follow‐up care; and complete clinical information. A total of 51 patients were included according to the criteria: 16 girls and 35 boys. Ages at operation averaged 5.9 ± 2.8 years. Outcome: anteroposterior (AP) and lateral X‐ray radiographs were performed every 2 weeks before the fracture healing, every 3 months until 1 year, then once a year after the surgery. Clinical examinations were recorded for any clinical deformity, hardware prominence, pain, and infection. ESIN were applied in either a retrograde or an antegrade fashion for 29 patients. For 22 patients, LCP were implanted. For the remaining 5 cases, instruments other than ESIN and LCP were applied.ResultsThe average follow‐up time was 29 months (range, 24–37 months). The average operation time was 78.2 min (range, 25–155 mins). The average blood loss in surgery was 69.6 mL (range, 3–700 mL). The average healing of fracture time was 2.2 month (range, 1–6 months). The average removal of implants time was 7.8 months (range, 3–20 months). The average expense for index operation was CNY 31 100 (CNY 17 500–142 200).Comminution grades were strongly correlated with time to fracture union (P < 0.001, r = 0.53) and time to implant removal (P = 0.006, r = 0.38). For comminuted pattern, the operation time and blood loss during operation in LCP were significantly higher than those in ESIN (P = 0.037 and P = 0.006, respectively). Other clinical parameters were similar between the two groups. No clinically detectable LLD, rotational or angular deformity was found. All patients recovered full knee range of motion. In this series, 30 patients (59%) had complications; 26 minor complications (51%) did not require unplanned surgery, most of which were prominence of hardware; 4 patients (8%) had a major complication and underwent an unplanned surgery. Of these, 2 were refractures distal to primary fracture and 2 were intolerable prominence of ESIN.ConclusionsElastic stable intramedullary nails provide equivalent outcomes but decreased operative time and blood loss during surgery, and lower hospitalization cost in treating low‐grade comminuted femur shaft fracture compared with plating techniques. The results of this study support the use of ESIN over plates in low‐grade comminuted femur shaft fractures despite the fact that both options are indicated.
Efficacy and Safety of Tranexamic Acid in Reducing Blood Loss of Lower Extremity Osteotomy in Peri‐acetabulum and High Tibia: A Systematic Review and Meta‐analysis
Authors: Yao Ru‐zhan , Gao Wei‐qiang , Wang Bing‐wu , Wang Guang‐lin , Wu Cheng‐xi , A‐mu Yi‐da 
Published: August 2019

ObjectiveTo assess the efficacy of tranexamic acid (TXA) in reducing total blood loss and transfusion, and the risk of thromboembolic events in patients undergoing periacetabular osteotomy (PAO) and high tibial osteotomy (HTO).MethodsA systematic literature search was performed using PubMed, the Cochrane Central Register of Controlled Trials (CENTRAL), Embase (Ovid), Medline (Ovid), and Web of Science. ClinicalTrials.gov, American Academy of Orthopaedic Surgeons (AAOS), and Orthopaedic Trauma Association (OTA) conference proceedings were also searched to gain more eligible studies. The primary outcome measure was total blood loss and the blood transfusion rate of the TXA group versus control. The meta‐analysis was conducted using the RevMan 5.3 and Stata 14.0 software.ResultsA total of six studies were included involving 665 patients. Three studies were PAO, and the other three were HTO. The total blood loss in PAO (WMD, −330.49; 95% CI, −390.16 to −270.83; P < 0.001) and HTO (WMD, −252.50; 95% CI, −356.81 to −148.18; P < 0.001) and hemoglobin decline (WMD, −0.74; 95% CI, −1.09 to −0.38; P < 0.001) were significantly less in the TXA group than in the control group. TXA could reduce transfusion rates in PAO (RR, 0.26; 95% CI, 0.09 to 0.75; P = 0.01) but had no effect on HTO (RR, 0.20; 95% CI, 0.01 to 4.10; P = 0.30). The wound complications (RR, 0.62; 95% CI, 0.13 to 2.94; P = 0.54) had no significant difference between TXA and control groups.ConclusionsThis meta‐analysis demonstrated that TXA reduces total blood loss and hemoglobin decline in patients undergoing PAO and is safe, but it has little benefit in regard to reducing transfusion rates or wound complications in HTO, so TXA might be unwarranted for routine use for HTO.
Polygenic risk scores in schizophrenia with clinically significant copy number variants
Authors: Taniguchi Satoru , Ninomiya Kohei , Kushima Itaru , Saito Takeo , Shimasaki Ayu , Sakusabe Takaya , Momozawa Yukihide , Kubo Michiaki , Kamatani Yoichiro , Ozaki Norio , Ikeda Masashi , Iwata Nakao 
Published: August 2019

AimsRecent studies have revealed that the interplay between polygenic risk scores (PRS) and large copy number variants (CNV; >500kb) is essential for the etiology of schizophrenia (SCZ). To replicate previous findings, including those for smaller CNV (>10kb), the PRS between SCZ patients with and without CNV were compared.MethodsThe PRS were calculated for 724 patients with SCZ and 1178 healthy controls (HC), genotyped using array‐based comparative genomic hybridization and single nucleotide polymorphisms chips, and comparisons were made between cases and HC, or between subjects with and without ‘clinically significant’ CNV.ResultsFirst, we replicated the higher PRS in patients with SCZ compared to that in HC (without taking into account the CNV). For clinically significant CNV, as defined by the American College of Medical Genetics (‘pathogenic’ and ‘uncertain clinical significance, likely pathogenic’ CNV), 66 patients with SCZ carried clinically significant CNV, whereas 658 SCZ patients had no such CNV. In the comparison of PRS between cases with/without the CNV, despite no significant difference in PRS, significant enrichment of the well‐established risk CNV (22q11.2 deletion and 47,XXY/47,XXX) was observed in the lowest decile of PRS in SCZ patients with the CNV.ConclusionAlthough the present study failed to replicate the significant difference in PRS between SCZ patients with and without clinically significant CNV, SCZ patients with well‐established risk CNV tended to have a lower PRS. Therefore, we speculate that the CNV in SCZ patients with lower PRS may contain ‘genuine’ risk; PRS is a possible tool for prioritizing clinically significant CNV because the power of the CNV association analysis is limited due to their rarity.
Mobile phone interventions to improve adolescents' physical health: A systematic review and meta‐analysis
Authors: Shin YunHee , Kim Sun Kyung , Lee Mihyun 
Published: August 2019

AbstractBackgroundWe conducted a systematic review and meta‐analysis to evaluate the effectiveness of mobile phone intervention in promoting a healthy lifestyle among adolescents.MethodsSix databases were used for literature searches: MEDLINE, Embase, Cochrane Library, Cumulative Index to Nursing and Allied Health Literature, KoreaMed, and Research Information Sharing Service. The keywords used were “adolescents,” “mobile phone,” “smartphone,” “mHealth,” “application,” and “text message.” The search was restricted to intervention studies using randomized controlled studies and pre‐ and post‐control group studies targeting adolescents, all written in English and Korean.ResultsEleven intervention studies, including 1,472 adolescents, were included. Included studies published between 2013 and 2018 used various mobile phone features: four studies utilized phone call and text messaging, whereas seven studies used health applications. Methodological quality was assessed and the overall quality of the studies was mid to low. The pooled data favored intervention in improving physical activity and reducing sugar‐sweetened beverage intake and screen time. In a pooled data analysis, the effectiveness of mobile phone intervention on body mass index and weight loss was not statistically significant.ConclusionThis study provided evidence that mobile phone intervention improved physical health and fitness among adolescents. More beneficial effects can be guaranteed when interventions involve informative and motivational messages. Strategies to ensure the durability of such interventions are needed to promote active and continuous engagement.
Territorial Autonomy as a Tool of Minority Accommodation in the Post‐Soviet Space: The Cases of Ukraine and Moldova
Authors: Osipov Alexander 
Published: August 2019

AbstractThe article examines how territorial autonomy arrangements provide for the accommodation of ethnic diversity in the framework of unconsolidated democracies or ‘hybrid’ regimes. The paper focuses on Ukraine and Moldova whose politics and techniques of government combine Soviet legacies with knowledge and values transferred from ‘old’ democracies. The article rests on institutional analysis and involves the study of institutional settings in the domains of instrumental and symbolic policies. The examined autonomy arrangements to a large extent follow the patterns inherited from the Soviet past such as non‐articulation of the autonomies’ ethnic underpinning, ‘fuzzy legality’, the prevalence of informal institutions and their symbiosis with the formal ones, and systemic discrepancies between symbolic and instrumental policies. The author argues that such a system can be functional and compatible with democratic governance.
Ethnic Entrepreneurship and Placemaking in Toronto's Ethnic Retail Neighbourhoods
Authors: Zhuang Zhixi Cecilia 
Published: August 2019

AbstractResearch about ethnic businesses primarily focuses on the urban context; yet, contemporary immigrants in North America have increasingly been settling and establishing new businesses in suburbs. This paper explores emerging suburban ethnic retail clusters in the Greater Toronto Area by comparing them to established urban business enclaves. Drawing on extensive field research, surveys, and interviews in more than 100 suburban Chinese and South Asian retail clusters, this paper explores entrepreneurial experiences in suburban retail spaces, the role of ethnic entrepreneurs in suburban placemaking, and the opportunities and constraints affecting entrepreneurs' interaction with other key players. It demonstrates the need to build on the mixed embeddedness model when exploring ethnic entrepreneurship in a suburban context, as well as the need to consider how the institutional framework plays a role in shaping ethnic retail places and the spatial and physical outcomes of ethnic entrepreneurship.