bluuebunny/crossref_metadata_2025_split huggingface dataset:
In silico Analysis of Different Signal Peptides for Secretory Production of Arginine Deiminase in Escherichia coli
Authors: Zarei Mahboubeh , Nezafat Navid , Morowvat Mohammad Hossein , Ektefaie Mohsen , Ghasemi Younes 
Published: January 2019

Background:
Secretory production of recombinant protein in bacterial hosts fulfills
several advantages. Selecting an appropriate secretory signal peptide is a critical step in secretory
production of different protein. Several patents report the usage of signal peptides for secretory
production of recombinant proteins in E. coli. In silico identification of suitable signal peptides is
a reliable and cost-effective alternative to experimental approaches.

Objective:
This study was aimed to predict best signal peptides for the secretory production of recombinant
arginine deiminase in E. coli.

Methods:
In this study, 30 different signal peptide sequences were retrieved from database. The
signal peptide probability, location of cleavage sites, and n, h and c regions were predicted by
SignalP 4.1 and Phobius servers. After purging the 30 predicted secretory signal peptides, TorT,
bla, NrfA, TolB, PapC, PldA, Lpp were removed. Several physicochemical properties of the remaining
potential SPs were determined by ProtParam, PROSO II, and SOLpro servers for theoretically
selecting the best candidates.

Results and Conclusion:
Based on physicochemical properties, the signal peptides of OmpC,
OmpF, and DsbA were identified respectively as the promising candidates for efficient secretory
production of arginine deiminase in E. coli. Although the computational approach has established
itself as a basis of modern biotechnology, the experimental study is necessary to validate its results.
The criteria used in this study could be applied to other targets for recombination processes.
IMPLEMENTATION OF ELECTROCATALYTIC REACTOR AS OXIDATION UNIT FOR RESIDUAL REAGENT WASTEWATER OF TESTING LABORATORY
Authors: Mukimin Aris , Wicaksono Kukuh Aryo , Zen Nur , Purwanto Agus , Vistanty Hanny 
Published: January 2019

The remaining reagent from the sample analysis process become a significant source of hazardous waste of laboratory tasting activities. Methylene blue, phenol and oil are pollutants common in the remaining reagent waste. The electrocatalytic reactor is effective oxidation units for these organic pollutants. The reactor was made for a 50 L capacity with cylindrical metal oxide as the anode. The three anode which 6 cm in diameter and 50 cm in length were paired stainless cathode with the distance of 2.5 cm. The reactor was also equipped with a stirrer that is connected to the motor so that the mass transfer and oxidizing agents is more effective. The reactor application was carried out by feeding the remaining reagent waste into the electrocatalytic unit and giving DC potential 5 Volt. Each COD content for reagent waste of detergent: 2864 mg/L, phenol: 838 mg/L and oil: 708 mg/L. The reactor has reduced COD to 2157 mg/L (detergent), 399 mg/L (phenol) and 506 mg/L (oil) for 120 minutes. The high COD content in residual is caused by solvent (chloroform or hexane) that used at extraction step in determining the process of a sample. This compound is tough to oxidize into CO2 by OH radical or hypochlorite acid formed at the anode during the electrolysis process
Influence of Operational Condition on the Performance of Halotolerant Enriched - Activated Sludge System for Treating Medium Salinity Peanut Roasted Wastewater.
Authors: Yuliasni Rustiana , Setianingsih Nanik Indah , Wicaksono Kukuh Aryo , Harihastuti Nani 
Published: January 2019

This research aimed to investigate the influence of operational condition on the performance of halotolerant enriched - activated sludge system for treating high organic wastewater with medium salinity from roasted peanut industry. Roasted peanut wastewater with VLR ranged from 0.268 to 4.7 kg COD/m3.day and Chloride concentration ranged between 1582 - 4392 mg/L was treated continuously for almost 77 days. Two identical reactor with Volume 25 L, namely R1 a conventional Activated Sludge (AS) System and R2, a halotolerant enriched-AS. Both reactors were running with operational condition: HRT (9 h to 46 h) and MLSS (1000-6000 mg/L). Compared to conventional AS system, Halotolerant enriched-Activated sludge system could remove average of 86.7% COD, compared with conventional AS which was 85.7%. Average COD effluent of Halotolerant Enriched-Activated Sludge was also considerably lower, which was 127 mg/L, than conventional AS which was 150 mg/L. Halotolerant enriched-activated sludge also produced less sludge, giving a high F/M ratio (4.9) compared with conventional AS (3.5). In order to make effluent fulfilled stream standard regulation (at central java region COD was150 mg/L), the favorable operational condition for both reactors would be at VLR 0.268 to 2.03 kg COD, HRT was 25 hours HRT, with MLSS was 2584 – 3956 mg/L and maximum chloride concentration 1920 mg/L.
SIMULTANEOUS TRACE LEVEL DETERMINATION OF BENZENE AND 1, 2-DICHLOROETHANE BY GC-HS/GC-MS IN SEVERAL PHARMACEUTICAL DRUG SUBSTANCES
Authors: Sultana Sayeeda , Nagarajan Balaji 
Published: January 2019

Objective: We herein report the simultaneous trace level determination of benzene and 1,2-dichloroethane in several active pharmaceutical substances by GC-HS (gas chromatograph-head space) using a DB-624 column.Methods: This GC-HS method was developed based on an oven-programmed approach using nitrogen gas as the mobile phase. Our method is also compatible with the GC-MS (gas chromatography-mass spectrometry) technique using helium as the mobile phase instead of nitrogen. The successful separation of benzene and 1,2-dichloroethane was established by confirmation of their corresponding specific molecular masses.Results: The retention time of benzene and 1,2-dichloroethane were found to be 34.8 min and 35.6 min, respectively. The linearity was found in the range of concentration of 0.63-4.22 ppm and 1.49-9.96 ppm for benzene and 1,2-dichloroethane. The detection limit and quantification limit for benzene were 0.2 and 0.6 ppm, while those of 1,2-dichloroethane were 0.6 ppm and 1.5 ppm. These values were calculated using our developed method with respect to the test concentration of 500 mg/ml. The recovery of benzene and 1,2-dichloroethane were found to be 89–110% and 91–105%, respectively for the various pharmaceutical drug substances. The specificity of the method was studied using 20 solvents which include benzene and 1,2-dichloroethane.Conclusion: We expect that our method will be applicable for the simultaneous trace level determination of benzene and 1,2-dichloroethane during the control of manufacturing processes, and for use in rapid analysis for quality control in the pharmaceutical industry. Finally, this method was validated according to the International Conference on Harmonization (ICH) Validation Guidelines Q2 (R1).
CARACTERIZAÇÃO DO TRAUMA EM VÍTIMAS SUBMETIDAS A PROCEDIMENTOS CIRÚRGICOS EM UM HOSPITAL PÚBLICO DA BAHIA
Authors: Brandão da Silva Bruna , Moreira de Almeida Rios Felipe , Dourado Araújo Thereza Cristina , Arraes Paz Maria do Socorro , Silva Gomes Xavier Aline , Santa Bárbara da Silva Silvone 
Published: January 2019

O objetivo deste estudo é caracterizar o trauma em vítimas submetidas a procedimentos cirúrgicos em um hospital público do estado da Bahia, Brasil. Trata-se de um estudo descritivo, de corte transversal, com abordagem quantitativa, realizado com vítimas de traumas submetidas a procedimentos cirúrgicos, admitidas no período de abril a junho de 2013 em um hospital público do interior do estado da Bahia. Os dados foram coletados em prontuários, por meio de um formulário estruturado. Os resultados mostraram que 81,4% eram do sexo masculino, 35,9% eram jovens com idade entre 18 e 28 anos e 65,6% eram solteiras. Com relação ao tipo de trauma, 79% das vítimas desenvolveram um único tipo de trauma, com predominância do trauma de extremidades (47,7%) e 21% tiveram politrauma. O principal mecanismo do trauma foi o acidente motociclístico (30,8%); 92,8% do total das vítimas foram submetidas a um procedimento cirúrgico; dentre as cirurgias realizadas, 40,3% foram ortopédicas, seguida de 23,8% torácicas. Concluiu-se que o principal tipo de trauma em vítimas submetidas a procedimentos cirúrgicos em um hospital público do estado da Bahia foi o de extremidades, sendo os acidentes de trânsito e a violência interpessoal as causas mais frequentes.
Palavras-chave: Traumatismo múltiplo. Ferimentos e lesões. Cirurgia. Epidemiologia.
CONSTRUÇÃO DE PROTÓTIPO PARA SISTEMATIZAÇÃO DA ASSISTÊNCIA DE ENFERMAGEM EM UMA UNIDADE DE CUIDADOS SEMI-INTENSIVOS
Authors: Barbosa Moura Tayse , Santa Barbara da Silva Santos Leonardo , Brandão Soares Eula Priscila , Santa Bárbara da Silva Silvone 
Published: January 2019

O processo de enfermagem é o instrumento metodológico utilizado para o processo de cuidar e a Sistematização da Assistência de Enfermagem implica na organização e execução desse processo. Este artigo tem como objetivo construir um protótipo-software para implantação da Sistematização da Assistência de Enfermagem em uma unidade de cuidados semi-intensivos de um hospital público da Bahia. Trata-se de uma pesquisa de intervenção, que busca modificar a realidade estudada, explicando os problemas e propondo soluções. Os resultados apontam que a prática de enfermagem inserida no contexto das práticas em saúde, enfrenta o desafio cotidiano de imprimir uma nova lógica à organização do trabalho, configurando um agir pautado na integralidade. Concluiu-se que a utilização de um software para implantação da Sistematização da Assistência em Enfermagem em um hospital público da Bahia irá facilitar a organização do trabalho da equipe de enfermagem, melhorando a qualidade da assistência prestada ao indivíduo.
Palavras-chave: Software. Processo de enfermagem. Cuidados de enfermagem.
La calidad sanitaria del chorizo rojo tradicional que se comercializa en la ciudad de Toluca, Estado de México
Authors: Becerril Sánchez Ana Laura , Dublán García Octavio , Domínguez-López Aurelio , Arizmendi Cotero Daniel , Quintero-Salazar Baciliza 
Published: January 2019

El chorizo rojo tradicional que se comercializa en Toluca es un producto cárnico con gran fama pero, poco se sabe sobre su calidad. El objetivo de este estudio fue determinar la calidad sanitaria de este embutido en diversos puntos de venta. A partir de tres muestreos, se analizaron 75 muestras de los cuatro principales mercados de la ciudad de Toluca y 10 carnicerías especializadas. Con base en normas oficiales mexicanas se determinaron: aw, humedad, pH, acidez y contenido de nitritos. Se realizó el conteo de bacterias mesófilas aerobias (BMA), bacterias ácido lácticas (BAL), coliformes totales y coliformes fecales; así como la detección de Salmonella spp. y Escherichia coli. El chorizo rojo tradicional no presentó diferencias significativas (P>0.05) en la mayoría de las variables fisicoquímicas y microbiológicas analizadas. Mostró un aw superior a 0.95; humedad entre 40 y 50 %; pH inferior a 5 y nitritos por debajo de los límites máximos (156 mg/kg) permitidos según normas oficiales mexicanas. El recuento de BMA fue entre 7.3 a 7.8 Log10 UFC/g y 7.8 a 8.1 Log10 UFC/g para BAL; coliformes totales entre 11.1 a 48.6 número más probable, NMP/g y coliformes fecales entre 4.9 a 9.7 NMP/g. La presencia de Salmonella spp. y E.coli spp. se detectó tanto en mercados como en carnicerías especializadas, independientemente de su naturaleza; y el porcentaje de incidencia varió entre 11.1 a 60 % y 16.7 a 43.3 %, respectivamente. El chorizo rojo tradicional de Toluca presenta características distintivas, pero es imperativo implementar programas de manejo higiénico-sanitario durante su producción, almacenamiento y comercialización, que garanticen la obtención de un embutido no sólo típico de la región, sino inocuo.
Penentuan Struktur Bawah Permukaan Daerah Geotermal Menggunakan Metode Geomagnet dan Geolistrik
Authors: Ritonga Wahyu Azhar , Kadri Muhammad 
Published: January 2019

Abstrak Telah dilakukan penelitian tentang Penentuan Struktur Bawah Permukaan Daerah GeothermalMenggunakan Metode Geomagnet Dan Geolistrik Di Dusun Bahoan Kecamatan Silau Kahean Kabupaten Simalungun. Penelitian ini bertujuan untuk mengetahui kontur penyebaran fluida, penampang anomali dan model lapisan struktur bawah permukaan. Pengukuran metode geomagnet menggunakan PPM (Proton Precession Magnetometer) tipe Elsec 770, pengambilan data dilakukan secara acak dengan jumlah titik yang diperoleh 40 titik ukur, pengolahan data menggunakan surfer 10 untuk mendapatkan peta kontur dan Mag2DCfor windows untuk mendapatkan penampang anomali magnetik. Metode geolistrik menggunakan alat Ares-G4 Versi 4,7 (Automatic Resistivity System) dan GPS (Global Position System) pengukuran metode geolistrik dilakukan dengan membentangkan elektroda serta menginjeksi arus melalui dua elektroda dan beda potensial yang muncul dapat terukur sehingga didapat harga resistivitas semu yang diperoleh dari alat geolistrik. Kemudian data diolah menggunakanRes2Div untuk mendapatkan penampang kontur 2D dari nilai resistivitas lapisan batuan. Hasil penelitian menunjukkan bahwa kontur penyebaran fluida menggunakan metode geomagnet dan geolistrik tersebar secara vertikal, penampang anomali dengan metode geomagnet memiliki nilai terendah 22,75 nT dan nilai tertinggi 69,92 nT, dari penampang anomali didapat nilai suseptibilitas 0,00054, 0,0006, 0,0016 dimana model lapisan struktur bawah permukaan terdiri dari batuan pasir, lempung dan gamping. Untuk metode geolistrik memiliki nilai resistivitas (0,00-100) dan model lapisan batuan lempung. Nilai resistivitas (150-200) model lapisan batuan lanau. Nilai restivitas (350 – 500) model lapisan pasir, dan nilai restivitas >2250 model lapisan batu gamping. Kata kunci: Geomagnet, Geolistrik, GPS, Suseptabilitas, Resistivity, Geothermal
Pengujian Sifat Mekanik Komposit Polypropilena (Pp) Daur Ulang Dengan Filler Serat Tanaman Lidah Mertua (Sansevieras Trifasciata)
Authors: Gaol Lisbet Lumban , Motlan Motlan 
Published: January 2019

Telah di lakukan penelitian pengujian sifat mekanik komposit yang menggunakan matriks polypropilena dengan filler serat tanaman lidah mertua (Sansevieras Trifasciata) yang bertujuan untuk membuat komposit polipropilena daur ulang dengan menggunakan serat tanaman lidah mertua serta mengetahui sifat mekanik uji tarik dan uji lentur dari komposit tersebut. Pada penelitian sebelumnya telah dilakukan penelitian tentang “ Sifat mekanik Komposit Polipropilena Daur Ulang Dengan Filler Serat Pandan Duri” (Ramotan, 2011). Dengan memvariasikan massa serat diperoleh nilai kekuatan tarik terbesar adalah komposit pada perbandingan fraksi massa (12,5:87,5)%, dengan tegangan maksimum rata rata 9,63 MPa, komposit yang memiliki kekuatan lentur terbesar adalah papan komposit tanpa serat dengan kekuatan lentur maksimum rata-rata 0,53 MPa. Kekuatan lentur yang lebih baik adalah pada perbandingan (2.5:92,5)% dengan kekuatan lentur maksimum rata-rata (UFSmaks) sebesar 0,43 MPa, perbandingan massa (7,5: 92,5)% memiliki kuat tarik dan lentur yang rendah. Maka saya tertarik untuk melakukan penelitian yang menggunakan matriks PP dengan filler tanaman lidah mertua. Dari hasil pengujian tarik diperoleh nilai terbesar (σmaks) pada komposit polypropilena dengan serat daun lidah mertua sebesar 26,61MPa yang terdapat pada perbandingan (20:80)% sedangkan nilai terendah (σmin) sebesar 14,54Mpa yang terdapat pada perbandingan (40:60)%. Dari hasil pengujian lentur diperoleh nilai terbesar ( ) pada komposit polypropilena dengan serat daun lidah mertua sebesar MPa yaitu pada perbandingan (0:100)% sedangkan nilai terendah ( ) pada komposit polypropilena dengan serat daun lidah mertua sebesar MPa yaitu terdapat pada perbandingan (60:40)%. Pencampuran yang baik antara matriks dengan filler yang digunakan sangat mempengaruhi nilai kekuatan tarik dan nilai kekuatan lentur. Kata Kunci : Sifat Mekanik, Polypropilena (Pp), Tanaman Lidah Mertua (Sansevieras Trifasciata)
Preliminary Study on Small Long-Life PWR Reactor Design Fueled Thorium with Protactinium as Burnable Poiso
Authors: Jubaidah Jubaidah 
Published: January 2019

Thorium as fissile material has overweigh advantages compared to Uranium in order to have a long-life PWR reactor without replacement fuel. Additional of Protactinium as a burnable poison is effective to reduce excess reactivity in the beginning of the reactor operation. This study aims to analyze the neutronic, burnup fuel, thermal-hydraulic and kinetic calculations in regard to reactor safety. The calculation is performed with SRAC program using JENDL-32 as data library. The results show that the value of keff for 33 years are still in the range 1.0154 <keff<1.0171 and the burn-up level has increased, due to the additional fissile material as conversion results of Th232 and Pa231. Axial linear power distribution indicates normal distribution pattern. The coolant temperature distribution in the axial direction is quite flat. Key words: PWR, Th-232, U-233, Pa-231, neutronic, thermal-hydraulic, burn-up level, reactor kinetics
KAJIAN TEORETIK UNTUK MENENTUKAN INDEKS BIAS DARI SEMIKONDUKTOR COPPER-PHTHALOCYANINE BERDASARKAN CELAH ENERGINYA
Authors: Siregar Alkhafi Maas , S Novi Febrina 
Published: January 2019

Penelitian ini dilakukan untuk menentukan indeks bias semikonduktor Cu-Phthalocyanine berdasarkan celah energinya. Penentuan indeks bias menggunakan metode semi empiris ZINDO/I dan perhitungan menggunakan pemrograman matlab. Pemodelan struktur diperoleh dengan memberikan masukan, yaitu unsur, senyawa, dan jumlah atom. Optimasi dilakukan menggunakan energi HOMO dan LUMO untuk memperoleh celah energi. Dengan menggunakan Persamaan Moss, Ravindra et al, Herve-Vandamme, Reddy, dan Kumar- Singh, nilai celah energi dapat menentukan nilai indeks bias. Pada energi 3,854757 eV untuk HOMO dan 3,538368 eV untuk LUMO, diperoleh celah energi 0,3164eV. Untuk menentukan nilai indeks bias, celah energi digunakan pada persamaan yang sesuai dengan ketentuan. Berdasarkan ketentuan dari setiap persamaan, nilai indeks bias yang sesuai pada celah energi 0,3164 eV adalah indeks bias dari persamaan Moss, dengan nilai indeks bias yaitu 4,1627. Kata kunci : semikonduktor, phthalocyanine, celah energi, indeks bias
Preparation and Characterization of Glucosamine Nanoparticle by Ionic Gelation Method Using Chitosan and Alginate
Authors: Prasetyo Yuli Agung , Rusdiana Taofik , Abdassah Marline 
Published: January 2019

Osteoarthritis is a chronic degenerative disease of the joints that usually treated by NSAID drugs in the long term leading to cardiovascular and gastrointestinal disorders. Glucosamine is a precursor in the formation of progression of joint which have not a significantly side effect. The problem in glucosamine administration occured when it is administered through the oral route resulting in first pass metabolism, while when it is administered via intavena route resulting in insulin resistance. Those problems can be solved by developing glucosamine into nanoglucosamine in order to increase the enzymatic stability which will protect the active ingredient from diminishing by the first pass effect hence the dose can be reduced, consequenlty it will reduce the insulin resistance, and increase the permeation. In this study, the nanoparticles of glucosamine with chitosan polymer and crosslinker alginate was prepared by the ionic gelation method with the principle of continued cross forming polyelectrolyte complexes. This study started from preformulation such as solubility and identify study by FTIR, then the formulations of chitosan: glucosamine: alginate = 5:1:1 (volume ratio) with the variation of concentration in the FI (chitosan: glucosamine: alginate = 0.08 %: 0.1%: 0.08%) and FII (chitosan: glucosamine: alginate = 0.1%: 0.1%: 0.08%). Results of nanoparticle characterization by particle size analyzer in the FI showed the better formula indicating a foggy coloid, no precipitation, the pH was 2.90±0.05, and the percent transmittance was 99.35%. The distribution of particle size, polydispersity index, and zeta potential for the formula I were 76.0 ± 21.8 nm; 0.300; and -0.30 mV, respectively. It could be concluded that the nanoparticle system of glucosamine can be better prepared from the 0.08% of chitosan, 0.1% of glucosamine and 0.08% of alginate.Keywords: alginate, chitosan, ionic gelation method, glucosamine nanoparticle
Monitoring of Moth Pests in Apple Tree Orchard
Authors: Paulen Oleg , Kobolka Radoslav 
Published: January 2019

Abstract
The work suggests importance of monitoring apple tree pests from moth group in growing conditions of Nitra, Slovakia. In 2014 there was observed occurrence of moths e.g. Codling moth (Cydia pomonella L.), Appleseed moth (Grapholita lobarzewskii Now.), Hawthorn berry moth (G. janthinana Dup.), and Summer fruit tortrix moth (Adoxophyes orana Fish. v. Roesl.) in the apple tree orchard located in the Botanical Garden of SUA in Nitra with help of pheromone traps. The date of first generation occurrence of Codling moth, Appleseed moth and Summer fruit tortrix moth was recorded on April 23. All the pests showed two peaks of flight activity, but with Hawthorn berry moth three periods of higher occurrence were recorded. The course of temperatures influenced number of pests trapped in traps remarkably. The number of pest individuals was highly influenced by rainy weather and lower temperatures in months when there was expected their highest harmfulness. The recorded values might be influenced by plant species diversity of the experimental orchard as well as that of the surrounding area.
Case Presentation: Unusual Association Between Possible Bilateral Intraventricular Xanthogranulomas, Postero-inferior Cerebellar Artery Aneurysm and Thrombophilia
Authors: Andone Sebastian Razvan , Romaniuc Andreea , Bajko Zoltan , Maier Smaranda , Barcutean Laura , Bălaşa Rodica 
Published: January 2019

Abstract

Introduction: Xanthogranulomas are rare, benign, usually asymptomatic, cutaneous tumors most frequently seen in children (juvenile xanthogranulomas). Some lesions can be found accidentally at randomly performed cerebral computer tomography (CT) or magnetic resonance imaging (MRI) or even on autopsy.

Case report: We present the case of a 44 year-old woman, known with a thrombophilic disorder (PAI-1 gene mutation, MTHFR C677T and A1298C) on chronic anticoagulant treatment. The onset of symptoms was in 2010, when she presented paresthesia and lower limbs weakness. Two years later the patient presents with severe intermittent headache and left hemicrania and a cerebral angio-MRI is performed showing a left postero-inferior cerebellar artery aneurysm and two choroid plexus intraventricular masses in the lateral ventricles. The patient developed a new symptom, dysarthria in 2014 and in 2015 has multiple episodes of loss of consciousness, interpreted as epileptic seizures. Routine blood tests were within normal range, except for a high cholesterol level. The patient was tested for autoimmune, infectious, endocrine and metabolic diseases that were negative. Surgical treatment and biopsy from the lesion was proposed, however the patient refused both procedures.

Conclusions: There is an association between xanthogranulomas localization and the choroid plexus, the most frequent CNS origin being in the trigon of the lateral ventricle. Our case does not resemble with any other case published, mostly because the unusual presentation, symptomatology and the association between xanthogranulomas, thrombophilia and postero-inferior cerebellar artery aneurysm which were never reported before in other cases of xanthogranulomas from the literature.