bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimized method for fluorine-18 radiolabeling of Affibody molecules using RESCA
Authors: Lechi Francesco , Eriksson Jonas , Odell Luke R. , Wegrzyniak Olivia , Löfblom John , Frejd Fredrik Y. , Zhang Bo , Eriksson Olof 
Published: October 2024

Abstract
Background
In recent years, the interest in Al[18F]F as a labeling agent for Positron Emission Tomography (PET) radiotracers has risen, as it allows for fast and efficient fluorine-18 labeling by harnessing chelation chemistry. The introduction of Restrained Complexing Agent (RESCA) as a chelator has also shown that chelator-based radiolabeling reactions can be performed in mild conditions, making the radiolabeling process attractively more facile than most conventional radiofluorination methods. The aim of the study was to establish optimized conditions for Al[18F]F labeling of Affibody molecules using RESCA as a complexing agent, using Z09591 and Z0185, two Affibody proteins targeting PDGFRβ and TNFα, respectively, as model compounds.

Results
The Al[18F]F labeling of RESCA-conjugated Z09591 was tested at different temperatures (rt to 60 °C) and with varying reaction times (12 to 60 min), and optimal conditions were then implemented on RESCA-Z0185. The optimized synthesis method was: 1.5–2.5 GBq of cyclotron produced fluorine-18 were trapped on a QMA cartridge and eluted with saline solution to react with 12 nmol of AlCl3 and form Al[18F]F. The respective RESCA-conjugated Affibody molecule (14 nmol) in NaOAc solution was added to the Al[18F]F solution and left to react at 60 °C for 12 min. The mixture was purified on a NAP5 size exclusion column and then analyzed by HPLC. The entire process took approximately 35 min, was highly reproducible, indicating the efficiency and reliability of the method. The labeled compounds demonstrated retained biological function for their respective targets after purification.

Conclusions
We present a general and optimized method for Al[18F]F labeling of RESCA-conjugated Affibody molecules, which can be widely applied to this class of peptide-based imaging agents. Moreover, radiochemical yields were improved when the labeling was conducted at 37 °C or above. In vitro and in vivo assessment of the respective tracers was promising, showing retained binding capacity as well as moderate defluorination, which is usually regarded as a potential downside for RESCA-conjugated tracers.

Graphical abstract
In silico identification and characterisation of pathogenic genetic variants (nsSNPs) in the eukaryotic initiation factors eIF2 and eIF2B affecting miRNA binding sites
Authors: Madasu Pavan K. , Lokesh Subrahmanyam V. , Chandran Thyageshwar 
Published: October 2024

Abstract
Background
The eukaryotic translation initiation factors (eIF2 and eIF2B) are known to play a regulatory role in translation initiation. Studies have indicated that several missense mutations in both eIF2 and eIF2B subunits can lead to severe neurological diseases and cancer. In the current study, we have attempted to identify and characterise the single-nucleotide polymorphisms (SNPs) in the said subunits and their correlation with various diseases.

Results
Interestingly, we could identify SNPs only in 3′ untranslated regions (3′UTR) from EIF2 (EIF2S1 and S3) and EIF2B (EIF2B1, B2 and B5 subunits). Of which, two SNPs, one in each EIF2B1 (rs1050448) and EIF2B2 (rs4556), are observed to be affecting miRNA binding sites. The gene ontology (GO) analysis of identified miRNAs indicates their association with central nervous system development, various stress responses, growth factors, and immune system signalling pathways. Furthermore, molecular docking studies also confirm that the identified miRNAs have an excellent binding ability with corresponding wild-type/mutant dsDNA and mRNA with HADDOCK binding scores in the range of − 38.78 to − 3.99 kcal/mol and − 86.47 to − 23.78 kcal/mol, respectively.

Conclusion
We conclude that the identified miRNAs may play a regulatory role in the symptomatic progression of neurological disorders and cancer and the same is validated by existing experimental evidences. Overall, the identified miRNAs serve as potential candidates for carrying out clinical investigations.

Graphical abstract
FSHB and FSHR genes variants in combination with hormones levels predict low and high ovarian response to controlled ovarian stimulation: a logistic regressive model
Authors: Lyangasova Olga V. , Lomteva Svetlana V. , Sagamonova Karina Y. , Butenko Elena V. , Shkurat Tatiana P. 
Published: October 2024

Abstract
Background
Predicting the number of follicles obtained after controlled ovarian stimulation (COS) is challenging, especially considering individual variability. Since FSH is a fundamental hormone that controls growing follicle activity, genetic variants are predicted to affect ovarian response to stimulation.

The aim of the study
The purpose of this study was to analyze whether FSHB rs10835638 and FSHR rs6166 genetic variants in combination with clinical parameters might be considered as potential precise predictors of ovarian response during COS.

Materials and methods
The present study included 144 women from infertile couples who underwent assisted reproductive technologies. Women with reduced FSH and/or AMH serum levels were excluded from the study. Genotyping was carried out applying restriction fragment length polymorphism analysis.

Results
Genotypes frequencies for FSHB rs10835638 and FSHR rs6166 were GG (73%), GT (24%), TT (3%) and AA (42%), AG (39%), GG (19%), respectively. FSHR rs6166 GG genotype was shown as associated with higher early follicular phase serum FSH, LH and progesterone levels, compared to AA and AG genotypes. Logistic regressive models that simultaneously use the patient’s genetic and clinical characteristics to calculate the probability of low or high ovarian response have been developed.

Conclusion
The present study suggests that rs10835638 and rs6166 genetic variants affect hypothalamic-pituitary–gonadal hormones serum levels, and together may provide an improved model for predicting an ovarian response during COS.
Egyptian pediatric kidney transplantation: highlights on post-transplant follow-up and management of complications by the Egyptian Pediatric Clinical Practice Guidelines Committee (EPG) Nephrology Group
Authors: Moustafa Bahia , Soliman Neveen A. , Shaheen Ihab , Badr Ahmed , Atia Fatma M. , El Hatw Mohamed K. , Mogahed Engy Adel , EL-Ghamrawy Mona , El Khashab Khaled , Shaheen Noha , Shouman Mohamad G. , Selim Abeer , El-Sharkawy Magdy , El Azeem Tarek Abd , Esmat Mohamad , Salman Manal , Ali-El-Dein Bedeir , Rahim Mona Abdel , El-Hadedy Muhamed A. , Ghoneim Moatasem Elsayed , Korkor Mai S. , Omar Tarek E. I. , Amer Yasser , Baky Ashraf Abdel ,   On Behalf of Egyptian Pediatric Clinical Practice Guideline Committee, Zeid Mohamed Helmy Abo , Halawa Ahmed , Saeed Bassam , Chen Yaolong 
Published: October 2024

Abstract
Background
Pediatric kidney transplantation is unique when compared to adults. They need optimum post-transplant care to achieve longer graft and patient survival. Infections, rejection, recurrence, malignancy, bone problems, hypertension, and surgical complications are the most important challenges that require prevention, early detection, and management. Chronic allograft nephropathy needs different approaches based on its related causes, allograft pathology, graft function, and availability of re-transplantation.

Aim/objectives
This national adapted guideline aims to frame evidence-based post-transplant recommendations adopted or adapted from EB source guidelines to optimize post-transplant patient care.

Methods
Our guideline is evidence based adapted from four national and international source guidelines with permissions [KDIGO 2020, RA/BTS 2022–2018, EAU 2018] that were appraised with AGREE II instrument tool. We followed the “adapted ADAPTE” CPG formal adaptation methodology that consists of 3 phases and 24 steps and tools. Virtual monthly meetings all through the year 2023 were activated for reviewing and validation of final adaptation of evidence-based guideline draft, considering all comments of external reviewers. This guideline was registered in the practice guideline registration for transparency (PREPARE), number: PREPARE-2023CN364.

Results
Recommendation statements in guidelines stand for results in articles. Discussion of important recommendations and the rationale behind selection of adopted statements and tailoring of others to suit our local facilities, expertise, and renal native disease profile was presented in the text with reasons and references.

Conclusion
The provided guidelines aim to improve graft and patient outcome through prevention and early management of complications and suggest research areas lacking validated research recommendations.
Bio-inhibitive corrosion effect of carica papaya leaf extract on cold-rolled mild steel in 0.1 M HCl solution
Authors: Iheaturu Nnamdi Chibuike , Ofoegbu Stanley Udochukwu , Aharanwa Bibiana Chimezie , Akanbi Magdalene Nkeiru , Udunwa Daniel Iheanacho , Ayo Kayode Olugbenga , Eke Augusta Ndidi , Ogunsemore Blessing Opeoluwa , Ndukwu Precious Oluebube , Umeh Chikaodinaka Ngozi 
Published: October 2024

AbstractPlant extracts are prone to microbial growth that can lead to microbial-induced corrosion (MIC), a major setback for their performance and stability as corrosion inhibitors. Hence, this work aims to identify the microbes that may cause the ineffectiveness of carica papaya leaf extract (CPLE) as a bio-corrosion inhibitor of cold-rolled mild steel in 0.1 M HCl solution for 48 days at 28℃. Cold-rolled mild steel coupons were immersed in 100 ml of 0.1 M hydrochloric (HCl) acid solution containing 1 g, 2 g, 3 g, 4 g, and 5 g of carica papaya leaf extract (CPLE) for 48 days at 28℃. The gravimetric method was used to determine the coupons' corrosion rate (CR), surface coverage, and inhibition efficiency (IE%) of the CPLE. The functional groups in the CPLE were identified using Fourier Transform Infrared Spectroscopy (FTIR), and the surface appearance of the coupons after immersion was examined using an atomic force microscope (AFM). Gravimetric data showed the coupons dipped in a 0.1 M HCl solution containing 3 g/100 ml of the inhibitor exhibited the highest inhibition efficiency of 87.73%, surface coverage of 0.88, and lowest corrosion rate of 0.00255 mm/day or 0.93 mm/yr, after 48 days at 28℃. In FTIR, transmittance peaks occurred at 1558 cm−1 for amines (-NH) in alkaloid scissoring, 1452 cm−1 for aromatic -C = C in flavonoids, 2932 cm−1 for aliphatic methylene (-CH2) and aldehyde (RCOH) stretching, 1035 and 1077 cm−1 for aromatic-OR in tannin, and 3280 cm−1 for out-of-plane bending of hydroxyl (-OH) in phenolics. Vitamin C (522,940 μg/g), flavonoids (860 μg/g), saponins (637.9 μg/g), terpenoid (48.4 μg/g), phenol (120%μg/g), alkaloid (4.18 μg/g), tannin (59.84%), and glycosides (9.04%) make up the extract's average phytochemical content. AFM presents pits and ridges on the mild steel coupons immersed in 0.1 M HCl solution without the extract. Those immersed in the solutions with varying concentrations of the extract had a passive layer of the CPLE adhering to the surfaces of the coupons thereby preventing the coupon’s direct contact with the acid solution and weight loss. However, over time, microbial growth decreased the efficiency of CPLE due to metabolites. Microbial tests show creamy, irregular and raised bacterial total colony-forming units per gram (Tcfu/g) as 4.6 × 105 Tcfu/g and 1.9 × 107 Tcfu/g in 2 runs. Also, a mixture of white and fluffy appearance and spore formation of fungi population of 4.8 × 105 Tcfu/g and 3.2 × 107 Tcfu/g in 2 Runs made up of Penicillium spp, Coccidioides immitis, Aspergillus flavus, and Fusarium spp, with hyphae, were seen in the solutions containing CPLE.
Average volume-assured pressure support versus fixed pressure support in chronic hypercapnic respiratory failure: a systematic review and meta-analysis
Authors: Evans Abbie , Alam Aayan , Nugent Kenneth 
Published: October 2024

Rationale: Chronic hypercapnic respiratory failure occurs due to alveolar hypoventilation resulting in carbon dioxide retention. This is commonly managed with noninvasive ventilation (NIV) with modalities including fixed pressure support and average volume-assured pressure support (AVAPS). However, there is limited information comparing outcomes with these two modes of ventilator support in the management of chronic hypercapnic respiratory failure.
Objective: This review and meta-analysis analyze the outcomes with fixed pressure NIV versus average volume-assured pressure support NIV in managing chronic obstructive pulmonary disease (COPD) with chronic hypercapnic respiratory failure, focusing on patients’ perception of symptom burden and gas exchange based on arterial blood gases.
Search methods: PubMed, Embase, Cochrane Central Register of Controlled Trials, and Web of Science databases were searched; the latest search date was December 1, 2023. Inclusion criteria: randomized control trials and crossover studies in English in adults over the age of 19 with the diagnosis of COPD and chronic hypercapnic respiratory failure. Exclusion criteria: patients less than 19 years old, patients with acute exacerbations, and patients with central respiratory failure or neuromuscular disease. Outcomes included blood gas analysis after use of NIV measured in mmHg and patient perception of mental health, symptom burden, and comfort. Results for each outcome were analyzed in RevMan using an inverse variance statistical method with a fixed effect analysis. The final analysis included 7 studies with 252 participants.
Results: The patients were 64 ± 9 years old. Baseline pulmonary function testing showed a forced expiratory volume in the first second (FEV1) of 34.6 ± 14.2 % predicted, consistent with severe COPD per GOLD criteria, and a baseline PaCO2 of 55.2 ± 9.2 mmHg. Primary outcomes for ventilation showed no statistical difference between AVAPS and fixed pressure support groups in PaCO2 (Odds Ratio [OR] -1.51; 95% Confidence Interval [CI]: -3.18, 0.16; p=0.08). Patient perceived outcomes were evaluated using several questionnaires, including St. George Respiratory Questionnaire (SGRQ), Short Form 36 Health Survey Questionnaire (SF-36), and Visual Analogue Scale (VAS). Comparable results were not available for all studies, but no statistically significant differences were found when comparing study results.
Conclusions: There was little or no clinically significant difference between fixed pressure support and AVAPS in gas exchange. There are inadequate data to draw conclusions about the effect of fixed pressure support compared to AVAPS on patient perceived outcomes, such as comfort and symptom burden. No studies evaluated mortality benefit, cost effectiveness, or hospitalizations.

Key words: COPD, chronic hypercapnic respiratory failure, non-invasive ventilation, average volume-assured pressure support ventilation
Scalable Quantum Photonic Devices Operating in the Telecom C-Band
Authors: Semenova Elizaveta 
Published: October 2024

We present the deterministic fabrication of quantum-dot photonic devices emitting high-purity single-photons in the telecom C-band. This will open scalable integration with photonic platforms to enable novel functionalities for on-chip quantum information processing.
Full-text article not available; see video presentation
Towards Room Temperature Quantum Repeating
Authors: Namazi Mehdi , Wang Yang , Craddock Alexandder , Mendoza Jaeda , flament Mael 
Published: October 2024

Here we will discuss our experimental results from entangling telecom photons generated using a four-wave mixing entanglement source with a room-temperature quantum memory. This work paves the way towards building field-deployable quantum repeaters.
Full-text article not available; see video presentation
Protocol for a qualiquantitative study of accessibility of sexual and reproductive health services among women with motor disabilities in Morocco
Authors: Zekaoui Firdaous , Boufettal Houssine , Aboutaieb Rachid 
Published: October 2024

Introduction
Approximately 1.3 billion people worldwide live with a disability, underlining the need for equitable health care for this group is important. Inclusive health systems are consistent with global health priorities, respond to United Nations sustainable development goals and economically viable. Despite advancements in Morocco’s health system and legal framework, significant disparities remains inaccessing sexual and reproductive health (SRH) services for women with disabilities. This study explores barriers and facilitators affecting access to SRH services for these women, aiming to provide recommendations for more inclusive services and improved health outcomes.

Methods
Using a mixed approach, this study assessSRH access for women with motor disabilities in two cities in Morocco, selected for their socio-demographic, cultural and and healthcare diversity. Quantitative data will be generated through questionnaires administrered to 400 participants, while qualitative information will come from 20 to 30 interviews. Recruitment involves working with health professionals, NGOs and social media campaigns to ensure a diverse sample. Data analysis includes NVivo for qualitative data and descriptive/inferential statistics for quantitative data.

Conclusion
The study aims to explore the perspectives of women with disabilities and health professionals regarding SRH services. It will provide recommendations for making SRH services more equitable and inclusive, ultimately improving health out comes and uphold health rights for women with disabilities.
Prevalence of symptomatic dry eye and influencing factors among Chinese adolescents: A cross-sectional study
Authors: Chen Xiaojuan , Zhou Yue , Gao Xian , Zhu Yan , Cai Qi , Yin Bianyu , Sun ZhiMin , Xiong Yaojia , Wang Yong , Huang Xiaobo 
Published: October 2024

Background
Comprehensive research on the impact of various types of refractive errors (RE) and anisometropia on dry eye disease is still lacking. This study aimed to estimate the prevalence rates and potential lifestyle factors related to symptomatic dry eye (SDE) among adolescents in eastern China.

Methods
A cross-sectional study was performed in 2023, and a stratified cluster sampling technique was used among adolescents in Nantong, China. Demographic information, including sex, age and BMI, were collected. All participants underwent optometric tests, while Ocular Surface Disease Index (OSDI) and self-designed questionnaires were administered. Both univariate and multivariate logistic regression analyses were used to assess associations between SDE and related parameters, and various types of RE and anisometropia were also included in the study.

Results
A total of 1,518 participants were enrolled in the study, and the overall prevalence of SDE was 20.3% among adolescents in Nantong, China. Multiple logistic regression analyses showed that high myopia (aOR = 3.42, 95% CI = 1.60–3.36, p = 0.025), frequent use of eye drops (aOR = 2.31, 95% CI = 1.60–3.36, p<0.001), a history of allergic conjunctivitis (aOR = 1.93, 95% CI = 1.09–3.34, p = 0.025), and frequent blinking (aOR = 3.23, 95% CI = 2.31–4.53, p<0.001) were identified as risk factors for SDE. Conversely, male gender (aOR: 0.76, 95% CI: 0.58–0.99, p = 0.043), increased sleep time (6–7 h: aOR = 0.64, 95% CI = 0.46–0.89, p = 0.009; 7–8 h: aOR = 0.64, 95% CI = 0.43–0.95, p = 0.026; >8 h: aOR = 0.43, 95% CI = 0.23–0.82, p = 0.010), and timely intervention when vision decline occurred were protective factors against SDE (aOR = 0.61, 95% CI = 0.43–0.85, p = 0.004).

Conclusion
High myopia was found to be independently associated with an increased risk of SDE., while hyperopia, astigmatism, and anisometropia were not independent risk factors for SDE. The identified risk and protective factors may help provide valuable insights for future research and interventions aimed at improving ocular health in adolescents.
پەیوەندی نائومێدی بە شێوەكانی توندوتیژی لای گەنج- لەژێر ڕۆشنایی تیۆرەكانی نائومێدی "ئەلبەرت كۆهەن" و "جۆن دولارد"
Authors: Mahmood Dastan O. , Anwar Basida H. 
Published: October 2024

ئامانجی سەرەكی ئەو توێژینەوەیە، بریتییە لە دۆزینەوەی پەیوەندی نێوان نائومێدی و شێوەكانی ڕەفتاری توندووتیژی، پێوانە كردندی گۆڕاوەكانی توێژینەوەكە و دەرخستنی رۆڵی گۆراوە دیمۆگڕافییەكان لەسەر ئەنجامەكانی توێژینەوەكە. بۆ شیكردنەوەی ئامانجەكان توێژەران سوودیان لە میتۆدی وەسفی بە ڕێگەی رووپێوی كۆمەڵایەتی بەنموونە وەرگرتووە، بۆ ئەو مەبەستەش فۆڕمێكی ڕاپرسیان ئامادەكردووە، كە لە پێوەری نائومێدی كە لە (28) بڕگە و پێوەری ڕەفتاری شێوەكانی توندووتیژیش لە (30) بڕگە پێكهاتبوو، كە تەواوی تایبەتمەندییە سایكۆسۆسیۆمەترییەكان بۆ پێوەرەكان ئەنجامدارەوە. هاوكات بە شێوەی هەڕەمەكی چینایەتی ناڕێژەیی نموونەیەكی ( 360) كەسی خوێندكارانی زانكۆی ڕاپەڕینیان وەرگرتوووە ، كە بە ڕێژەی یەکسان (180) کەس لە ڕەگەزی مێ و (180) کەس ڕەگەزی نێر وەرگیراون. بۆ شیكردنەوەی داتا بە دەستهاتووەكانیش توێژەران سوودیان لە پرۆگرامی (spss) وەرگرتووە.
گرنگترین ئەو ئەنجامانەی ئەم توێژینەوەیە پێی گەیشتووە، برتییە لەوەی : بەشێوەیەكی گشتی نائومێدی و شێوەكانی ڕەفتاری توندووتیژی لای نموونەی توێژینەوەكە لە ئاستێكی زۆر نزمدایە. هەروەها دەركەوتووە جیاوازیەكی بەڵگەداری ئاماری لە نێوان ڕەگەزی نێر و مێدا سەبارەت بە نائومێدی هەیە، بەجۆرێك ڕەگەزی نێر بەبەراورد بە ڕەگەزی مێی زیاتر نائومیدی لایان بوونی هەیە. هاوكات ڕەگەزی نێر بەبەراورد بە ڕەگەزی مێی زیاتر ڕەفتاری توندووتیژی( جەستەیی و هێمای) ئەنجام دەدەن، بەڵام سەبارەت بە توندووتیژی (زارەكی) زیاتر ڕەگەزی مێی ئەنجامی دەدات بەبەراورد بە ڕەگەز نێر. هەروەها دەركەوتووە ئەو خوێندکارانەی ئاستی ئابووری خێزانیان خراپە زیاتر نائومێدیان لا دەركەوتووە. لەكۆتایشدا دەركەوتووە پەیوەندیەكی بەڵگەداری ئاماری ڕاستەوانەی مامناوەند لە نێوان نائومێدی و توندووتیژی بە گشتی و توندووتیژی (جەستەیی و زارەكی و هێمای)دا هەیە. واتە هەرچەندی ئاستی نائومێدی بەرزبێتەوە ئەوا شێوەكانی ڕەفتاری توندووتیژیش بەرزدەبێتەوە. بە پێچەوانەشەوە ڕاستە.
Context‐specific anti‐inflammatory roles of type III interferon signaling in the lung in nonviral injuries
Authors: Feng Jingjing , Kim Jooyoung , Wang Victoria D. , Chang De , Liu Hongbo , Bain William G. , Robinson Keven M. , Jie Zhijun , Kotenko Sergei V. , Dela Cruz Charles S. , Sharma Lokesh 
Published: October 2024

AbstractType III interferons (λ1, λ2, and λ3) are potent antiviral cytokines in the lung. However, their roles in nonviral lung injuries are less well understood. This study investigates the activation of type III interferon signaling in three distinct models of lung injuries caused by diverse stimuli: the bacterial pathogen Pseudomonas aeruginosa, bacterial endotoxin LPS, and the chemotherapeutic agent bleomycin. Our data show that, despite inducing a potent inflammatory response, Pseudomonas and LPS did not increase IFNλ secretion. In contrast, bleomycin instillation increased secretion of IFNλ in the airways at both early and late time points. Consistent with limited secretion, type III interferon signaling had a minimal role in the host response to both Pseudomonas and LPS, as measured by pathogen burden, inflammatory response, and lung injury. Conversely, a deficiency in type III interferon signaling led to increased inflammatory signaling and elevated acute lung injury in the bleomycin model on day 3. This elevated early injury resulted in impaired recovery in IFNLR1 knockout mice, as evidenced by their recovery from bleomycin‐induced weight loss. Taken together, these data suggest a context‐specific activation of type III interferon signaling, where it plays an anti‐inflammatory role in the lung.