bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED: Long non‐coding RNA TGLC15 advances hepatocellular carcinoma by stabilizing Sox4
Authors: Chen Yang , Huang Fei , Deng Liang , Tang Yajun , Li Dong , Wang Tielong , Fan Youwen , Tao Qiang , Tang Di 
Published: September 2019

AbstractBackgroundThe hepatocellular carcinoma (HCC) belongs to a common malignancy especially in China. Recent data have clarified important roles of long non‐coding RNAs (lncRNAs) in HCC. However, the role of a novel intergenic lncRNA termed TGLC15 is still elusive.MethodsWe screened for novel lncRNAs using lncRNA profiling. TGLC15 expression was quantified by qRT‐PCR. In vitro experiments such as migration and viability assays were performed. In vivo implantation experiments were conducted to investigate tumorigenic functions of TGLC15. Combined RNA immunoprecipitation (RIP) and mass spectrometry (MS) were utilized to uncover Sox4 as TGLC15 binding protein.ResultsTGLC15 is significantly overexpressed in tumor tissues and HCC cell lines. Higher TGLC15 levels correlated with advanced malignant characteristics such as TNM stages, tumor size, and metastasis. TGLC15 advanced HCC migration and viability. The in vivo experiments supported that xenograft tumor growth and proliferation were facilitated by TGLC15 overexpression. Mechanistic studies showed that TGLC15 interacted with Sox4 and interaction between TGLC15 and Sox4 could stabilize Sox4 via reduction in proteasome‐mediated degradation.ConclusionsCollectively, our data have identified a novel lncRNA TGLC15 during HCC development. The TGLC15‐Sox4 signaling might be a potential target for pharmaceutical intervention.
Next‐generation sequencing identifies a novel frameshift variant in FRMD7 in a Chinese family with idiopathic infantile nystagmus
Authors: Wang Fengqi , Guan Hongzai , Liu Wenmiao , Zhao Guiqiu , Liu Shiguo 
Published: September 2019

AbstractBackgroundIdiopathic infantile nystagmus (IIN) is a high genetically heterogeneous ophthalmic disease and is often associated with pathogenic mutations in FRMD7 and GPR143, respectively. Idiopathic infantile nystagmus manifests as involuntary periodic rhythmic oscillation of the eyes in the very early life, which decreases visual acuity and affects the quality of life.Objective and MethodsThe aim of our study was to reveal a possible pathogenic variant through the investigation of a Chinese Han family with IIN with an implementation of a next‐generation sequencing method. Isolated DNA analysis was followed by Sanger sequencing validation. We also performed the detailed ophthalmological examination of family members.ResultsWe identified a novel frameshift variant in FRMD7 (NM_194277.2: c.1419_1422dup, p.Tyr475fs), which leads to a frameshift mutation since tyrosine (Tyr) at 475 codon of FRMD7 protein (p.Tyr475fs) and co‐segregates with IIN phenotype in this family.ConclusionsWe found a novel frameshift FRMD7 variant in a Chinese Han family, which may be causative variant for IIN and can further enrich the mutation spectrum and uncover the etiology of IIN.
Increased effectiveness of urinalysis testing via the integration of automated instrumentation, the lean management approach, and autoverification
Authors: Wongkrajang Preechaya , Reesukumal Kanit , Pratumvinit Busadee 
Published: September 2019

AbstractBackgroundIn 2014, the Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand developed and implemented a new process that uses fully automated instrumentation, the lean management approach, and autoverification to improve the productivity and efficiency of the urinalysis workflow process. The aim of this study was to evaluate analytical turnaround time compared with our old urinalysis workflow process and our new urinalysis workflow process that was launched in 2014.MethodsThis study was performed at the Central Laboratory of our center during June 2017 using data collected from the July 2012 (old process) and July 2014 (new process) study periods. We used our laboratory information system to compute and analyze turnaround time of urinalysis tests, and those results were compared between processes.ResultsThe 90th percentile turnaround time in overall data was dramatically decreased from approximately 60 minutes in 2012 to <50 minutes in 2014. The mean during both 6:00 am to 9:00 am and 9:00 am to 12:00 pm was approximately 42 minutes in 2012; however, that duration was reduced to approximately 30 minutes for both of those time periods in 2014. Specimens within 60 minutes in both intervals increase from approximately 80% to more than 90%.ConclusionThe results of this study revealed our new urinalysis workflow process that incorporates fully automated instrumentation, the lean management approach, and autoverification to be effective for significantly increasing productivity as measured by analytical turnaround time and removing 1 staff to another section.
Both HuR and miR‐29s regulate expression of CB1 involved in infiltration of bone marrow monocyte/macrophage in chronic liver injury
Authors: Chang Na , Duan Xianghui , Zhao Zhongxin , Tian Lei , Ji Xiaofang , Yang Lin , Li Liying 
Published: September 2019

AbstractBone marrow–derived monocytes/macrophages (BMMs) play a vital role in liver inflammation and fibrogenesis. Cannabinoid receptor 1 (CB1) mediates the recruitment of BMMs into the injured liver. In this study, we revealed the molecular mechanisms under CB1‐mediated BMM infiltration. Carbon tetrachloride (CCl4) was employed to induce mouse liver injury. In vivo, human antigen R (HuR) was upregulated in macrophages of injured liver. HuR messenger RNA (mRNA) expression was positively correlated with CB1 and F4/80 mRNA expression. Furthermore, we detected the binding between HuR and CB1 mRNA in CCl4‐treated livers. In vitro, HuR modulated arachidonyl‐2′‐chloroethylamide (ACEA, CB1 agonist)‐induced BMM migration by regulating CB1 expression. HuR promoted CB1 expression via binding to CB1 mRNA. ACEA promoted the association between HuR and CB1 mRNA via inducing HuR nucleoplasmic transport. In the cytoplasm, HuR competed with the miR‐29 family to improve CB1 expression and BMM migration. In conclusion, our results prove that HuR regulates CB1 expression and influences ACEA‐induced BMM migration by competing with miR‐29 family.
HPV16+‐miRNAs in cervical cancer and the anti‐tumor role played by miR‐5701
Authors: Pulati Nuerbieke , Zhang Zegao , Gulimilamu Aireti , Qi Xiaoli , Yang Jie 
Published: September 2019

AbstractBackgroundThe early diagnosis of cervical cancer is difficult, resulting in an unsatisfactory prognosis. The present study aimed to explore the expression of HPV16‐related microRNAs (miRNAs) in the serum of Uygur cervical cancer in Sinkiang and analyze the miRNAs showing different expression.MethodsThe serum of 30 HPV16 positive (HPV16Pos) and 30 negative patients (HPV16Neg) was collected and then the differentially expressed miRNAs were screened out by function and pathway enrichment analyses. Next, the cervical cancer cells were cultured. miR‐5701‐mimic and inhibitor were transfected into cells. The level of miR‐5701 was detected by a quantitative reverse transcriptase‐polymerase chain reaction. The proliferation of cells was detected using a Cell Counting Kit‐8 assay. Western blotting was used to measure the expression of THBS4.ResultsWe identified three up‐regulated miRNAs (hsa‐miR‐1291, hsa‐miR‐144‐5p and hsa‐miR‐5701) and seven down‐regulated known‐miRNAs (hsa‐miR‐21‐5p, hsa‐miR‐101‐3p, hsa‐miR‐370‐3p, hsa‐miR‐151a‐3p, hsa‐miR‐144‐3p, hsa‐miR‐199a‐3p and hsa‐miR‐199b‐3p) relative to HPV16Neg. The mitogen‐activated protein kinase signal pathway is predicted to be a key mechanism of HPV16‐related cervical cancer. Furthermore, miR‐5701 inhibits the proliferation of cervical cancer cells and suppresses the expression of THBS4 (P < 0.05), which was confirmed as a target gene of miR‐5701.ConclusionsIn the present study, we confirm 10 differentially expressed miRNAs that could be potential markers or therapeutic targets of cervical cancer. miR‐5701 inhibits the proliferation of cervical cancer cells and the expression of its target gene THBS4.
The cascade of care for children and adolescents with HIV in the UK and Ireland, 2010 to 2016
Authors: Chappell Elizabeth , Lyall Hermione , Riordan Andrew , Thorne Claire , Foster Caroline , Butler Karina , Prime Katia , Bamford Alasdair , Peters Helen , Judd Ali , Collins Intira J ,   On behalf of the Collaborative HIV Paediatric Study Steering Committee
Published: September 2019

AbstractIntroductionThe UNAIDS 90‐90‐90 targets for the cascade of care are widely used to monitor the success of HIV care programmes but there are few studies in children. We assessed the cascade for children and adolescents living with HIV in the national Collaborative HIV Paediatric Study (CHIPS) in the UK and Ireland.MethodsUtilizing longitudinal data from CHIPS we compared the cascade of care for 2010, 2013 and 2016. Among children diagnosed with HIV and not known to be lost to follow‐up at the start of each calendar year, we summarized the proportion in active paediatric care during that year (defined as having ≥1 clinic visit, CD4 or viral load measurement, or change to antiretroviral therapy (ART) regimen), and of these, the proportion on ART at last visit in that year. Among those on ART, the proportion with viral suppression (<200 copies/mL) and good immune status (WHO immunological stage none‐/mild‐for‐age) at last visit in the year were summarized. Among those in care in 2016, outcomes were compared by current age, place of birth (born abroad vs. UK/Ireland) and sex.ResultsOf children in paediatric HIV care at the start of 2010, 2013 and 2016 (n = 1249, 1157, 905 respectively), the proportion in active care during that calendar year was high throughout at 97 to 99%. Of those in active care, the proportion on ART increased from 79% to 85% and 92% respectively (p < 0.001). Among those on ART, the proportion with viral suppression and good immune status was stable at 83% to 86% and 85% to 88%, respectively, across the years. Among children in care in 2016, those aged ≥15 years were less likely to be virally suppressed (79% vs. 91%, p < 0.001) or to have good immune status (78% vs. 94%, p < 0.001) compared to younger children; there were no differences by place of birth or sex.ConclusionsChildren and adolescents in the UK and Ireland national cohort had high retention in care. The proportion on ART increased significantly over time although there was no change in viral suppression or good immune status. Poorer outcomes among adolescents highlight the need for targeted support for this population.
Genetic and antigenic characteristics of a human influenza C virus clinical isolate
Authors: Liu Runxia , Sheng Zizhang , Lin Tao , Sreenivasan Chithra , Gao Rongruan , Thomas Milton , Druce Julian , Hause Ben M. , Kaushik Radhey S. , Li Feng , Wang Dan 
Published: September 2019

AbstractUnlike influenza A and B viruses that infect humans and cause severe diseases in seasonal epidemics, influenza C virus (ICV) is a ubiquitous childhood pathogen typically causing mild respiratory symptoms. ICV infections are rarely diagnosed and less research has been performed on it despite the virus being capable of causing severe disease in infants. Here we report on the isolation of a human ICV from a child with acute respiratory disease, provisionally designated C/Victoria/2/2012 (C/Vic). The full‐length genome sequence and phylogenetic analysis revealed that the hemagglutinin‐esterase‐fusion (HEF) gene of C/Vic was derived from C/Sao Paulo lineage, while its PB2 and P3 genes evolved separately from all characterized historical ICV isolates. Furthermore, antigenic analysis using the hemagglutination inhibition (HI) assay found that 1947 C/Taylor virus (C/Taylor lineage) was antigenically more divergent from1966 C/Johannesburg (C/Aichi lineage) than from 2012 C/Vic. Structure modeling of the HEF protein identified two mutations in the 170‐loop of the HEF protein around the receptor‐binding pocket as a possible antigenic determinant responsible for the discrepant HI results. Taken together, results of our studies reveal novel insights into the genetic and antigenic evolution of ICV and provide a framework for further investigation of its molecular determinants of antigenic property and replication.
Lack of Marseillevirus DNA in immunocompetent and immunocompromised Italian patients
Authors: Macera Lisa , Spezia Pietro Giorgio , Focosi Daniele , Mazzetti Paola , Antonelli Guido , Pistello Mauro , Maggi Fabrizio 
Published: September 2019

AbstractMarseilleviridae is a family of viruses which have only been propagated in acanthamoeba. Marseillevirus sequences have been recently detected in different human matrices by viral metagenomics. Single‐center studies worldwide have estimated a low prevalence of marseillevirus both in symptomatic patients and in healthy donors but, to date, no informations are available on the prevalence of this giant virus in Italy. By a polymerase chain reaction targeting the ORF152 viral sequence, we tested sera from 197 immunosuppressed patients and 285 healthy donors, and 63 and 30 respiratory and cerebrospinal fluid samples, respectively, of patients with various clinical conditions and referring the Virology Division for diagnostic purposes. We observed no evidence of Marseillevirus DNA in all 575 samples tested. Marseillevirus probably does not cause infection in human.
Can primitive kimberlite melts be alkali‐carbonate liquids: Composition of the melt snapshots preserved in deepest mantle xenoliths
Authors: Golovin A.V. , Sharygin I.S. , Korsakov A.V. , Kamenetsky V.S. , Abersteiner A. 
Published: September 2019

AbstractThe study of kimberlite rocks is important as they provide critical information regarding the composition and dynamics of the continental mantle and are the principal source of diamonds. Despite many decades of research, the original compositions of kimberlite melts, which are thought to be derived from depths > 150 km, remain highly debatable due to processes that can significantly modify their composition during ascent and emplacement. Snapshots of the kimberlite‐related melts were entrapped as secondary melt inclusions hosted in olivine from sheared peridotite xenoliths from the Udachnaya‐East pipe (Siberian craton). These xenoliths originated from 180‐ to 220‐km depth and are among the deepest derived samples of mantle rocks exposed at the surface. The crystallised melt inclusions contain diverse daughter mineral assemblages (>30 mineral species), which are dominated by alkali‐rich carbonates, sulfates, and chlorides. The presence of aragonite as a daughter mineral suggests a high‐pressure origin for these inclusions. Raman‐mapping studies of unexposed inclusions show that they are dominated by carbonates (>65 vol.%), whereas silicates are subordinate (<13 vol.%). This indicates that the parental melt for the inclusions was carbonatitic. The key chemical features of this melt are very high contents of alkalis, carbon dioxide, chlorine, and sulfur and extremely low silica and water. Alkali‐carbonate melts entrapped in xenolith minerals likely represent snapshots of the primitive kimberlite melt. This composition is in contrast with the generally accepted notion that kimberlites originated as ultramafic silicate water‐rich melts. Experimental studies revealed that alkali‐carbonate melts are a very suitable diamond‐forming media. Therefore, our findings support the idea that some diamonds and kimberlite magmatism may be genetically related.
Unique Solubility of Switchable Alkyl‐Amine Surfactants in Water
Authors: Cui Leyu , Lagorse Marine , Bourrel Maurice 
Published: September 2019

AbstractBecause many amine surfactants are soluble in both water and CO2 phases, they attract interest with regard to stabilizing CO2‐in‐water dispersion systems. In our recent research, we find that the solubility of alkyl‐amine surfactant in water can be significantly enhanced by salts, even though the salts are usually “salting‐out” to other surfactants. The influence of various anions (NO3−, Br−, Cl−, and SO42−) and cations (Na+, Ca2+, and Mg2+) on the alkyl‐amine surfactants is investigated. The results are contrary to Hofmeister series and show that all the anions can enhance the solubility (salting‐in) in the order of: NO3− > Br− > Cl− > SO42−, while the impact of the cations is insignificant. A physical–chemistry model based on the switchable property of the surfactant is proposed and well explains the experimental results. Therefore, the switchable alkyl‐amine surfactants have potential to be applied under high‐salinity and high‐temperature conditions, for example, in enhanced oil recovery processes for a hot and salty carbonate reservoir.
Predictors of voice outcome in pediatric non‐selective laryngeal reinnervation
Authors: Ongkasuwan Julina , Espinosa Maria Catalina L. , Hollas Sarah , Devore Danielle , Procter Teresa , Bassett Ethan , Schwabe Aloysia 
Published: September 2019

ObjectivesNon‐selective laryngeal reinnervation (NSLR) using the ansa cervicalis to the recurrent laryngeal nerve (RLN) is a promising treatment option for pediatric unilateral neuronal vocal fold movement impairment (VFMI). The aim is to describe our clinical outcomes with this technique and to identify preoperative characteristics that may predict postoperative voice outcomes.MethodsThis is a cohort study of pediatric patients with unilateral neuronal VFMI, who underwent NSLR from March 2012 to July 2018. Pre‐ and postoperative Pediatric Voice Related Quality of Life (PVRQOL) questionnaires, Consensus Auditory Perceptual Evaluation of Voice (CAPE‐V) ratings, and objective voice measures were obtained. In addition, patients underwent preoperative laryngeal electromyography (LEMG).ResultsThirty‐two patients were identified. Twenty‐one had complete data sets for analysis. The mean duration of VFMI was 9.02 years (range 1.1–26.1 years). There were significant improvements in PVRQOL (P = .0005), in all CAPE‐V subsets (P ≤ .0001 to .0195), mean and maximum intensities (P = .0342 and 0.0110, respectively), cepstral peak prominence (P = .0001), and cepstral spectral index of dysphonia (P ≤ .0001). A worse preoperative LEMG correlated with a greater change in maximum phonation time (P = .0162) and maximum intensity (P = .0346). Age at injury and duration of injury had no significant impact on voice outcomes; however, patients with concurrent posterior glottic insufficiency did have smaller changes in PVRQOL (P = .012).ConclusionNSLR is an effective treatment for pediatric unilateral neuronal VFMI even many years after initial RLN injury. LEMG may help predict voice outcomes of reinnervation in pediatric patients, but further data is still needed.Level of Evidence4 Laryngoscope, 130:1525–1531, 2020
Transformation between localized and general corrosion behavior of die‐cast AZ91D magnesium alloy in 3.5 wt% NaCl aqueous solutions
Authors: Zhang Shaohua , Hou Lifeng , Du Huayun , Wei Huan , Liu Baosheng , Wei Yinghui 
Published: September 2019

AbstractTransformation behavior between localized and general corrosion of die‐cast AZ91D magnesium alloy in anodizing processes was investigated by galvanostatic polarization. The results revealed that, at electric current density 5, 10, and 20 mA/cm2, a localized corrosion state appears on the specimen surface in the initial stage (10 min), and is changed into general corrosion with the galvanostatic measurement time. On the other hand, the increase in electric current density has caused the increase of the corroded area on the specimen surfaces at the same galvanostatic measurement time. In comparison, the appearance of localized corrosion based on general corrosion can be attributed to the localized absorption along with enhanced activity and reactivity of Cl− at the higher electric current density (10 and 20 mA/cm2). Meanwhile, the penetration of Cl− throughout the corrosion products has caused the destruction of the integrity of these corrosion products, which promotes the continuous growth of the localized corrosion pits under the galvanic effect.
Laser structured nitride and oxynitride coatings deposited by middle frequency magnetron sputtering
Authors: Bobzin K. , Brögelmann T. , Kruppe N.C. , Naderi M. 
Published: September 2019

AbstractIm Rahmen dieser Untersuchungen galt es zu bestimmen, inwiefern die Variation der Laserstrahlparameter, wie z. B. Pulsenergie und Pulsanzahl, Einfluss auf die Eigenschaften von unbeschichteten Stahlsubstraten sowie von nitridischen und oxinitridischen Beschichtungen nimmt. Drei verschiedene Sauerstoff‐Gasflüsse j(O2) = 10 sccm, j(O2) = 15 sccm und j(O2) = 20 sccm im Beschichtungsprozess und entsprechend drei verschiedene resultierende Beschichtungszusammensetzungen mit xj(O2) = 10 sccm = (39,8±4,2) Atom‐%, xj(O2) = 15 sccm = (31,6±1,4) Atom‐% und xj(O2) = 20 sccm = (58,4±6,0) Atom‐% wurden untersucht. Die Analyse der Strukturtiefe mittels konfokaler Laserscanningmikroskopie zeigte, dass mit steigender Pulsenergie und Pulsanzahl die Strukturtiefe erhöht wird. Die linienartigen Interferenzstrukturen mit konstanter Periodizität wurden mittels Rasterelektronenmikroskopie erfasst. Zusätzlich sind bei allen beschichteten Oberflächen in vertikaler Richtung laserinduzierte periodische Oberflächenstrukturen mit höherer räumlicher Frequenz und kleinerer Periodizität deutlicher zu beobachten. Bei der Beschichtung mit xj(O2) = 20 sccm = (58,4±6,0) Atom‐% entstehen nach der Laserstrukturierung an den Flanken der Interferenzstruktur Mikrorisse.
Robust numerical implementation of a 3D rate‐dependent model for reservoir geomechanical simulations
Authors: Isotton Giovanni , Teatini Pietro , Ferronato Massimiliano , Janna Carlo , Spiezia Nicolò , Mantica Stefano , Volonte Giorgio 
Published: September 2019

SummaryA 3D elasto‐plastic rate‐dependent model for rock mechanics is formulated and implemented into a Finite Element (FE) numerical code. The model is based on the approach proposed by Vermeer and Neher (A soft soil model that accounts for creep. In: Proceedings of the International Symposium “Beyond 2000 in Computational Geotechnics,” pages 249‐261, 1999). An original strain‐driven algorithm with an Inexact Newton iterative scheme is used to compute the state variables for a given strain increment.The model is validated against laboratory measurements, checked on a simplified test case, and used to simulate land subsidence due to groundwater and hydrocarbon production. The numerical results prove computationally effective and robust, thus allowing for the use of the model on real complex geological settings.
Teacher power and children's loneliness: Moderating effects of teacher‐child relationships and peer relationships
Authors: Zhang Feng , Jiang Ying , Lei Xiuya , Huang Silin 
Published: September 2019

AbstractTeacher‐child relationships and peer relationships are important predictors of children's loneliness. However, few studies have examined the potential and adverse relationship between teacher power and children's loneliness. Thus, we explicitly explored whether teacher power is related to children's loneliness and examined the potential moderating roles of interpersonal relationships (i.e., teacher‐child relationships and peer relationships) in 888 Chinese children. Moreover, this study measured teacher power with children's figure drawings. The results showed that teacher power was significantly and positively related to children's loneliness, and both teacher‐child relationships and peer relationships moderated the link between the two variables. Specifically, the adverse correlation between teacher power and children's loneliness was not significant among children with high‐quality teacher‐child relationships. Similarly, the negative effect of teacher power on children's loneliness was not found among children with a high level of peer relationships. Therefore, our results confirmed that the adverse relationship between teacher power and children's loneliness, and the protective roles of high quality of teacher‐child relationships and high levels of peer relationships in this relationship. Potential implications and future research directions are discussed.