bluuebunny/crossref_metadata_2025_split huggingface dataset:
Up‐regulation of the pentose phosphate pathway and HIF‐1α expression during neural progenitor cell induction following glutamate treatment in rat ex vivo retina
Authors: Tokuda Kazuhiro , Baron Byron , Yamashiro Chiemi , Kuramitsu Yasuhiro , Kitagawa Takao , Kobayashi Masaaki , Sonoda Koh‐Hei , Kimura Kazuhiro 
Published: August 2019

AbstractThe metabolic state influences the regulation of neural stem/progenitor cells. The pentose phosphate pathway (PPP), an alternative metabolic pathway that operates parallel to glycolysis, not only provides key intermediates for biosynthetic reactions but also controls the fate of neural stem/progenitor cells. We have previously shown that glutamate application leads to the induction of neural progenitor cells in mature ex vivo rat retina. In this study, we investigated whether regulation of the PPP might be changed following glutamate treatment of the retina. Immunoblot analysis revealed that the amount of glucose‐6‐phosphate dehydrogenase (G6PD), the rate‐limiting enzyme of the PPP as well as that of 6‐phosphogluconate dehydrogenase (6PGD), another enzyme in this pathway, increased in the glutamate‐treated retina. Consistent with the fact that both these enzymes generate reduced nicotinamide adenine dinucleotide phosphate (NADPH), the amount of NAPDH in the treated retina was significantly higher compared with that in the untreated retina. We also found that both DNA synthesis as well as the expression of fatty acid synthase (FASN) increased significantly in the glutamate‐treated retina. Furthermore, hypoxia‐inducible factor 1‐α (HIF‐1α), a positive transcriptional regulator of PPP enzymes, was up‐regulated at both messenger RNA (mRNA) and protein levels. Finally, we found the interaction of HIF‐1α with the M2 isozyme of pyruvate kinase (PKM2), with this interaction having been shown to contribute to a positive feedback loop in the control of glycolysis. Our results thus show that specific metabolic change in the PPP occurs in the process of neural progenitor cell induction in the mature rat retina.
km23‐1/DYNLRB1 regulation of MEK/ERK signaling and R‐Ras in invasive human colorectal cancer cells
Authors: Raza Asif , Pandey Madhu S. , Jin Qunyan , Mulder Kathleen M. 
Published: August 2019

AbstractWe previously found that km23‐1/DYNLRB1 is required for transforming growth factor‐β (TGFβ) production through Ras/ERK pathways in TGFβ‐sensitive epithelial cells and in human colorectal cancer (CRC) cells. Here we demonstrate that km23‐1/DYNLRB1 is required for mitogen‐activated protein kinase kinase (MEK) activation in human CRC cells, detected by km23‐1/DYNLRB1‐siRNA inhibition of phospho‐(p)‐MEK immunostaining in RKO cells. Furthermore, we show that CRISPR‐Cas9 knock‐out (KO) of km23‐1/DYNLRB1 reduced cell migration in two additional CRC models, HCT116 and DLD‐1. Of interest, in contrast to our previous work showing that dynein motor activity was required for TGFβ‐mediated nuclear translocation of Smad2, in the current report, we demonstrate for the first time that disruption of dynein motor activity did not reduce TGFβ‐mediated activation of MEK1/2 or c‐Jun N‐terminal kinase (JNK). Moreover, size exclusion chromatography of RKO cell lysates revealed that B‐Raf, extracellular signal‐regulated kinase (ERK), and p‐ERK were not present in the large molecular weight fractions containing dynein holocomplex components. Furthermore, sucrose gradient fractionation of cell lysates from both HCT116 and CBS CRC cells demonstrated that km23‐1/DYNLRB1 co‐sedimented with Ras, p‐ERK, and ERK in fractions that did not contain components of holo‐dynein. Thus, km23‐1/DYNLRB1 may be associated with activated Ras/ERK signaling complexes in cell compartments that do not contain the dynein holoprotein complex, suggesting dynein‐independent km23‐1/DYNLRB1 functions in Ras/ERK signaling. Finally, of the Ras isoforms, R‐Ras is most often associated with cell migration, adhesion, and protrusive activity. Here, we show that a significant fraction of km23‐1/DYNLRB1 and RRas wase co‐localized at the protruding edges of migrating HCT116 cells, suggesting an important role for the km23‐1/DYNLRB1‐R‐Ras complex in CRC invasion.
RNA‐binding protein SFPQ cooperates with HDAC1 to suppress CD40 transcription in pulmonary adventitial fibroblasts
Authors: Pan Yan‐Yun , Yang Jin‐Xiu , Mao Wei , Wang Xing‐Xiang 
Published: August 2019

AbstractPulmonary artery adventitial fibroblasts, the most abundant cellular constituent of adventitia, are often the first to be activated and reprogrammed to then influence the tone and structure of the vessel wall in pulmonary arterial hypertension (PAH). Our previous study found that interruption of CD40 ligand (CD40L)‐CD40 signaling improves the efficacy of transplanted endothelial progenitor cells in monocrotaline induced‐PAH. However, whether CD40L‐CD40 signaling is involved in the activation of adventitial fibroblasts in PAH and whether Drosophila behavior human splicing (DBHS) protein family members have any roles during adventitial fibroblasts activation are completely unclear. Here, we show that soluble CD40L (sCD40L) stimulation progressively increases pro‐inflammatory activity, proliferation, and migration of pulmonary adventitial fibroblasts. Besides, sCD40L stimulation decreases splicing factor proline‐ and glutamine‐rich protein (SFPQ) protein (one member of DBHS protein family) expression, while SFPQ overexpression suppresses sCD40L stimulation‐induced proliferation and migration of pulmonary adventitial fibroblasts by repressing CD40 transcription. Moreover, ChIP assays found that sCD40L stimulation promotes histone H3 tri‐methylation at lysine 4 (H3K4me3), H3K36me3, and H3K27 acetylation (H3K27ac) modifications on CD40 promoter region in pulmonary adventitial fibroblasts, while SFPQ overexpression decreases H3K36me3 modification and increases H3K36ac on CD40 promoter region by interacting with histone deacetylase‐1 (HDAC1) to inhibit CD40 transcription. This in‐depth study shows that CD40L‐CD40 signaling promotes activation of pulmonary adventitial fibroblasts by increasing proliferation, migration, and pro‐inﬂammatory activity of adventitial fibroblasts, and SFPQ could inhibit CD40 transcription though switching H3K36me3 to H3K36ac modifications on its promoter by interacting with HDAC1. This study, first, uncovers the roles of SFPQ on CD40L‐CD40 signaling‐mediated activation of pulmonary adventitial fibroblasts.
Cardamonin induces G2/M arrest and apoptosis via activation of the JNK–FOXO3a pathway in breast cancer cells
Authors: Kong Weiwei , Li Chuang , Qi Qiaofang , Shen Jiahui , Chang Kaiwen 
Published: August 2019

AbstractCardamonin (CD), a naturally occurring chalcone isolated from large black cardamom, was previously reported to suppress the proliferation of breast cancer cells. However, its precise molecular anti‐tumor mechanisms have not been well elucidated. In this study, we found that CD markedly inhibited the proliferation of MDA‐MB 231 and MCF‐7 breast cancer cells through the induction of G2/M arrest and apoptosis. Reactive oxygen species (ROS) plays a pivotal role in the inhibition of CD‐induced cell proliferation. Treatment withN‐acetyl‐cysteine (NAC), an ROS scavenger, blocked CD‐induced G2/M arrest and apoptosis in this study. Quenching of ROS by overexpression of catalase also blocked CD‐induced cell cycle arrest and apoptosis. We showed that CD enhanced the expression and nuclear translocation of Forkhead box O3 (FOXO3a) via upstream c‐Jun N‐terminal kinase, inducing the expression of FOXO3a and its target genes, including p21, p27, and Bim. This process led to the reduction of cyclin D1 and enhancement of activated caspase‐3 expression. The addition of NAC markedly reversed these effects, knockdown of FOXO3a using small interfering RNA also decreased CD‐induced G2/M arrest and apoptosis. In vivo, CD efficiently suppressed the growth of MDA‐MB 231 breast cancer xenograft tumors. Taken together, our data provide a molecular mechanistic rationale for CD‐induced cell cycle arrest and apoptosis in breast cancer cells.
Photoactive Polyoxometalate/DASA Covalent Hybrids for Photopolymerization in the Visible Range
Authors: Boulmier Amandine , Haouas Mohamed , Tomane Somia , Michely Laurent , Dolbecq Anne , Vallée Anne , Brezová Vlasta , Versace Davy‐Louis , Mialane Pierre , Oms Olivier 
Published: August 2019

AbstractThe synthesis of TBA‐DASA‐POM‐DASA, the first photoactive covalent hybrid polyoxometalate (POM) incorporating a donor–acceptor Stenhouse adduct (DASA) reverse photochrome, is presented. It has been evidenced that in solution the equilibrium between the colorless cyclopentenone and the highly colored triene conformers is strongly dependent not only on the nature of the solvent but also the countercations, allowing to tune its optical properties. This complex has been further associated to photochromic spironaphtoxazine cations, resulting in a material which can be activated by two distinct optical stimuli. Moreover, when combined with N‐methyldiethanolamine, TBA‐DASA‐POM‐DASA constitutes a performing photoinitiating system for polyethylene glycol diacrylate polymerization and under visible light irradiation, a promising result in a domain scarcely developed in POM chemistry.
A Multi‐crosslinking Nanocapsule‐Based Serial‐Stimuli‐Responsive Leakage‐Free Drug‐Delivery System In Vitro
Authors: Liu Yazhou , Shen Tianjiao , Gao Congcong , Abdulhadi El‐Ali H. A. , Gao Na , Yang Chunlei , Zhang Rubo , Jing Jing , Zhang Xiaoling 
Published: August 2019

AbstractAs some stimuli utilized in conventional drug delivery systems can also be found in normal cells, it is inevitable that encapsulated drugs escape from carriers into normal cells. Based on mutual interactions among proteins, polyphenol compounds, and metal ions, we developed a serial‐stimuli‐responsive drug delivery system. With multi‐crosslinking structure, nanocapsules can maintain the integrity of the framework, even with a certain amount of stimuli present, and eventually reach tumor cells to initiate apoptosis, and protect normal cells from being damaged. Meanwhile, the fluorescence of DOX will be quenched when encapsulated in nanocapsules. This property means that the DOX that is released from nanocapsules can be monitored in real‐time based on the recovery of fluorescence. These versatile nanocapsules exhibit great potentials to treat cancer.
Phosphole‐Fused Dehydropurpurins via Titanium‐Mediated [2+2+1] Cyclization Strategy
Authors: Higashino Tomohiro , Nishimura Issei , Imahori Hiroshi 
Published: August 2019

AbstractA [2+2+1] cyclization strategy of bis(alkynyl)porphyrin using low‐valent titanium species, generated in situ, afforded phosphole‐fused dehydropurpurins for the first time. The systematic investigations on the electronic properties of the dehydropurpurins revealed their unique features owing to the oxidation states of the phosphorus atom on the fused phosphole ring. The phosphole P=O and P=S derivatives were found to possess high electron‐accepting character derived from phosphorus(V) centers without the contribution of 24π antiaromatic character, suggesting their potential utility as electron‐accepting materials. In contrast, the phosphorus(III) derivatives revealed different optical and electrochemical properties arising from both 18π aromatic and 24π antiaromatic networks including the lone pair of the phosphorus(III) atom. Overall, the oxidation state of the phosphorus atom has a clear impact on the whole electronic properties, demonstrating the advantages of chemical modifications of the phosphorus center for creating an exotic π‐system. The application of titanium‐mediated [2+2+1] cyclization to porphyrins is highly promising for expanding a world of heterole‐fused porphyrinoids.
Pentafluoroethylation of Arenediazonium Tetrafluoroborates Using On‐Site Generated Tetrafluoroethylene
Authors: Xing Bo , Li Lingchun , Ni Chuanfa , Hu Jinbo 
Published: August 2019

Summary of main observation and conclusionCopper‐mediated pentafluoroethylation of arenediazonium tetrafluoroborates with tetrafluoroethylene (TFE) on‐site generated from TMSCF3 has been developed as a new method to prepare pentafluoroethyl arenes. The active pentafluoroethylation reagent “CuC2F5” is pre‐generated from CuSCN, TFE and CsF, and its generation and further reaction are strongly solvent‐dependent. This pentafluoroethylation reaction represents the first example of Sandmeyer‐type pentafluoroethylation, which exhibits good functional group tolerance and potential applications for the synthesis of complicated bioactive compounds.
Assessment of Sertoli Cell Proliferation by 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide and Sulforhodamine B Assays
Authors: Martins Ana D. , Oliveira Pedro F. , Alves Marco G. 
Published: August 2019

AbstractThe correct functioning of Sertoli cells (SCs) is pivotal for successful spermatogenesis. They are major targets for hormones, endocrine disruptors, and other substances that men are subjected to every day. One of the main SC functions that quickly responds to a deleterious stimulus is proliferation. This is directly related to the in vivo capacity of these cells to sustain a good number of developing germ cells. The protocols in this article can be tested on SCs of different origin. For the case of human SCs from small human testicular biopsies, a short and simple protocol to isolate and culture these cells is provided. The other protocols discussed herein represent two different procedures, somewhat complementary, to assess SC proliferation. In brief, the sulforhodamine B assay allows the investigator to dye healthy fixed SCs maintained in culture. In the MTT assay, on the other hand, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) is reduced by live SCs. These methods are mostly used to evaluate how SC proliferative activity responds to exposure to compounds such as toxicants or hormones. © 2019 by John Wiley & Sons, Inc.
A holistic approach to manage environmental quality by using the Kano model and social cognitive theory
Authors: Dace Elina , Stibe Agnis , Timma Lelde 
Published: August 2019

AbstractSince its first proposition in 1984, the Kano model has been used extensively in a variety of contexts within industries and academic research demonstrating its wide applicability. The Kano model allows for describing the relationship between an objective aspect and a subjective aspect. Yet is this relevant for environmental quality as well? In this study, we explore the cases where the Kano model is used for assessing environmental quality and its perception by consumers and identify the potential influencing factors for its application with this respect. We find that the Kano model can serve as an effective tool for converging towards environmental quality and sustainability by indicating the direction from a social and behavioral choice perspective. We propose an integration of the Kano model with Bandura's triangle (a concept of the social cognitive theory) to create an instrument for driving change of customer attitude and behavior via changing the perception of environmental quality.
Corporate financial performance due to sustainable development in Vietnam
Authors: Tien Nguyen Hoang , Anh Dinh Ba Hung , Ngoc Nguyen Minh 
Published: August 2019

AbstractSocial responsibility and environmental sustainability issues began to be paid attention in Vietnam due to the impressive economic development in recent years. However, corporate social responsibility (CSR) is still quite a new management concept, especially for small and medium enterprises. Managers are facing multiple obstacles in executing CSR programs in accordance with international standards. Regarding corporate environmental sustainability (CES), the idea of sustainable development is a rising trend in today's business reality. Essentially, CSR is regarded as a part of the overall sustainability issues. For the purpose of studying the impact of CSR and CES on corporate financial performance (CFP), quantitative method has been used to examine the relation between CSR and CFP. Qualitative method has been applied to verify the relation between CSR, CES, and CFP. On the basis of findings, several useful suggestions are made for managers to improve business performance and sustainable development in Vietnam.
RAD‐EI: A routing attack detection scheme for edge‐based Internet of Things environment
Authors: Wazid Mohammad , Reshma Dsouza Poonam , Das Ashok Kumar , Bhat K Vivekananda , Kumar Neeraj , Rodrigues Joel J. P. C. 
Published: August 2019

SummaryInternet of Things (IoT) offers various types of application services in different domains, such as “smart infrastructure, health‐care, critical infrastructure, and intelligent transportation system.” The name edge computing signifies a corner or edge in a network at which traffic enters or exits from the network. In edge computing, the data analysis task happens very close to the IoT smart sensors and devices. Edge computing can also speed up the analysis process, which allows decision makers to take action within a short duration of time. However, edge‐based IoT environment has several security and privacy issues similar to those for the cloud‐based IoT environment. Various types of attacks, such as “replay, man‐in‐the middle, impersonation, password guessing, routing attack, and other denial of service attacks” may be possible in edge‐based IoT environment. The routing attacker nodes have the capability to deviate and disrupt the normal flow of traffic. These malicious nodes do not send packets (messages) to the edge node and only send packets to its neighbor collaborator attacker nodes. Therefore, in the presence of such kind of routing attack, edge node does not get the information or sometimes it gets the partial information. This further affects the overall performance of communication of edge‐based IoT environment. In the presence of such an attack, the “throughput of the network” decreases, “end‐to‐end delay” increases, “packet delivery ratio” decreases, and other parameters also get affected. Consequently, it is important to provide solution for such kind of attack. In this paper, we design an intrusion detection scheme for the detection of routing attack in edge‐based IoT environment called as RAD‐EI. We simulate RAD‐EI using the widely used “NS2 simulator” to measure different network parameters. Furthermore, we provide the security analysis of RAD‐EI to prove its resilience against routing attacks. RAD‐EI accomplishes around 95.0% “detection rate” and 1.23% “false positive rate” that are notably better than other related existing schemes. In addition, RAD‐EI is efficient in terms of computation and communication costs. As a result, RAD‐EI is a good match for some critical and sensitive applications, such as smart security and surveillance system.
The independent and interacting effects of weight suppression and admission body mass index on treatment weight change in patients with anorexia nervosa or bulimia nervosa
Authors: Piers Amani D. , Espel‐Huynh Hallie M. , Lowe Michael R. 
Published: August 2019

AbstractObjectiveWeight suppression (WS) and body mass index (BMI) have predicted weight change in individuals with eating disorders, but the interaction between these variables is understudied. Furthermore, WS is usually measured as absolute WS—the numeric difference between current weight and highest past weight—overlooking the potentially important influence of how much a person weighed at their highest historical weight.MethodThe current study investigated the independent and interacting effects of BMI and two measures of WS at admission on residential treatment weight change. WS measures included absolute WS and the relative WS index, the percentage of total body weight lost from highest past weight. Participants were women with anorexia nervosa (n = 357) or bulimia nervosa (n = 293) who provided complete data, 87% of the eligible treatment sample.ResultsIn both diagnostic subsamples, BMI, absolute WS, and the relative WS index all significantly predicted weight change. The interaction between BMI and WS predicted weight change, but only when the relative WS index was used.DiscussionResults highlight the potential importance of considering an individual's weight and weight history when predicting their treatment weight change and support the importance of utilizing both methods of calculating WS in future research.
Self‐assembly of p‐Aminothiophenol on Gold Surface: Application for Impedimetric and Potentiometric Sensing of Cobalt (II) Ions – A Comparative Study
Authors: Kaur Sarbjeet , Kaur Inderpreet 
Published: August 2019

AbstractCobalt, despite an essential biological element, imposes threat to humans when exposed to high concentration or even to low concentration for long term which demands the development of highly sensitive and selective analytical methods for its trace analysis. In the present work, self‐assembly of p‐aminothiophenol (p‐ATP) on gold surface (Au−ATP SAM) was carried out and for the first time, applied as a platform for impedimetric and potentiometric sensing of Co2+. Au−ATP SAM was characterized using electrochemical techniques: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), in the presence of two redox probes: [Fe(CN)6]3−/4− and [Ru(NH3)6]2+/3+ to evaluate associated passivating behaviour. Au−ATP SAM completely blocked [Fe(CN)6]3−/4− as compared to [Ru(NH3)6]2+/3+ which may be attributed to inner‐sphere and outer‐sphere ET mechanisms, respectively. Au−ATP SAM was found to exhibit excellent sensitivity towards Co2+ in a wider concentration range from 1.0×10−12 M to 1.0×10−5 M (r2=0.963) at pH 5.5 with a detection limit of 6.0×10−13 M and superior selectivity. Further, carbon paste electrode (CPE) was prepared by incorporating p‐ATP bound gold nanoparticles and explored for potentiometric sensing of Co2+ which exhibited Nernstian slope of 29.2±0.2 mV/dec in linear concentration range of 1.0×10−6 M–1.0×10−1 M (r2=0.971) with a detection limit of 8.0×10−7 M. The proposed sensors were successfully applied for estimation of Co2+ content in water samples.
Experimental study on the seepage characteristics of bituminous coal under the conditions of liquid nitrogen fracturing
Authors: Chen Shuai , Zhang Lei , Zhang Cun , Huang Mengqian 
Published: August 2019

AbstractIn coalbed methane (CBM) exploitation, liquid nitrogen (LN2) fracturing has attracted much attention as a promising and effective means to enhance coal seam permeability. Considering the bituminous coal in the Xutuan coal mine of Anhui Province, China, the permeability characteristics of coal samples under different pressure and stress conditions, and a single LN2 treatment and cyclic fracturing conditions were studied. Additionally, an evaluation of the macroscopic cracking behavior of coal was carried out. LN2 treatment can be very effective in promoting the generation of macroscopic fissures in the coal, and the degree of damage increases with the number of fracturing cycles. The results of a single LN2 treatment showed that under a constant confining pressure, the permeability increased exponentially with the increase in gas pressure. The cyclic LN2 treatment showed that permeability first decreased and then increased with increasing gas pressure, which is consistent with the quadratic function distribution. The permeability of the coal sample increases exponentially with the number of cycles, but the growth rate gradually slows down. For the same absolute fracturing time, cyclic fracturing had a better fracturing effect than a single cycle, and the ratio of the permeability increment is 126.3%‐213.8%. The research results provide some guidance for the development of the LN2 freeze fracturing technology.