bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seismogeological Criteria for the Gas Potential of Aptian–Cenomanian Sediments in the North of West Siberia (by the Example of the Yubileinoe Field)
Authors: Kontorovich V.A. , Surikova E.S. , Ayunova D.V. 
Published: June 2019

Abstract
—Seismogeological criteria for the gas potential of the Aptian–Cenomanian sediments of West Siberia are substantiated. The research was carried out by the method of integrated interpretation of seismic materials, well logs, well test results, and petrophysical studies. As a reference object we used the Yubileinoe oil and gas condensate field located in the Nadym–Pur interfluve, in which a unique gas deposit is concentrated in the Cenomanian reservoir PK1 and the Albian sand bed PK18. Based on the research results, seismogeologic criteria for gas prediction have been formulated. Massive Cenomanian gas pools are displayed in wave seismic fields by: (1) the presence of reflectors formed at the gas–water contacts in time sections and a decrease in the amplitude parameters of the reflector G confined to the roof of Cenomanian sequence; (2) an increase in the time thickness (ΔT), a decrease in the interval velocities (Vint), and a decrease in the amplitude–energy parameters of the seismic record in the Aptian–Cenomanian sequence. Aptian–Albian sheet gas pools are displayed in the wave fields by a sharp increase in the amplitudes of the seismic record, forming “bright spot” anomalies in the time sections.
Kultuminskoe Gold–Copper–Iron–Skarn Deposit (Eastern Transbaikalia, Russia): Petrogeochemical Features of Magmatism and Ore-Forming Processes
Authors: Kovalev K.R. , Kalinin Yu.A. , Turkina O.M. , Gimon V.O. , Abramov B.N. 
Published: June 2019

Abstract
—The Kultuminskoe deposit is located within the Gazimur metallogenic zone in eastern Transbaikalia. Mineralization is associated with the Middle–Upper Jurassic Kultuma pluton composed of subalkaline rock series ranging from quartz monzonites and quartz syenites to granites and of monzodiorite dikes. Dikes of Late Jurassic age are composed of subalkaline gabbro. Analysis of fractionation trends of major and trace elements suggests that the monzonitoids prevailed in the Kultuma pluton and the dike complex formed through the differentiation of subalkaline basaltic melt from an enriched mantle source. The formation of the gold–copper–iron–skarn and medium-temperature veinlet-disseminated polysulfide and epithermal Ag–Te–Bi mineralization as well as iron–magnesia and silica–alkaline meta-somatites was a long multistage process during the general evolution of the ore-magmatic system.
High-Grade Contact Metamorphism in the Kochumdek River Valley (Podkamennaya Tunguska Basin, East Siberia): Evidence for Magma Flow
Authors: Sokol E.V. , Polyansky O.P. , Semenov A.N. , Reverdatto V.V. , Kokh S.N. , Devyatiyarova A.S. , Kolobov V.Yu. , Khvorov P.V. , Babichev A.V. 
Published: June 2019

Abstract
—Spurrite-merwinite marbles on the right bank of the Kochumdek River in the Podkamennaya Tunguska basin formed along the top margin of a flood basalt intrusion (Kuzmovka complex) from a marly limestone protolith of the Rhuddanian Lower Kochumdek Subformation, at a pressure of ~200 bars. The contact metamorphic aureole comprises four zones of successively decreasing temperatures marked by the respective mineral assemblages: T ≥ 900 °C (merwinite, spurrite and gehlenite (±rankinite, bredigite); T ≥ 750 °C (spurrite); T ≥ 700 °C (tilleyite, wollastonite, and melilite (Gehl&lt;50)); and ~500–550 °C (diopside, amphibole, and grossular). Very high temperatures at the contact (Tcont &gt; 2/3 Tmelt) result from magma flow along a conduit. The temperature profiles for the Kochumdek metamorphic complex show good fit between measured and geothermometer-derived values at a magma temperature of 1200 °C, an intrusion thickness of ≥ 40 m, a heating time of six months, and a magma flow lifespan within one month. Stagnant magma in a conduit of any thickness cools down and crystallizes rapidly and fails to heat up sediments to the temperatures required for spurrite–merwinite metamorphism (above 790 °C).
Effect of disintegrates and metabolites of Lactobacillus rhamnosus and Saccharomyces boulardii on biofilms of antibiotic resistant conditionally pathogenic and pathogenic bacteria
Authors: Isayenko O. Y. , Knysh O. V. , Babych Y. M. , Ryzhkova T. N. , Dyukareva G. I. 
Published: June 2019

The work presented here is the first to examine the impact of Lactobacillus rhamnosus GG ATCC 53103 and Saccharomyces boulardii metabolites obtained using the author`s method on the formation of biofilm forms of bacteria. The structural components of the probiotic microorganisms were obtained using the method of physical disintegration – low frequency ultrasound waves produced by a G3-109 generator. Metabolites were obtained by cultivating L. rhamnosus and S. boulardii in ultrasound disintegrates of lactobacteria and Saccharomycetes. The impact of biologically active substances on the formation of biofilm of Corynebacterium ulcerans tox+ 112, C. diphtheriae gravis tox+ 108, by antibiotic-resistant Pseudomonas aeruginosa PR, Klebsiella pneumoniae PR, Lelliottia amnigena (Enterobacter amnigenus) PR and P. aeruginosa AТСС 27853 reference strain was studied using the spectrophotometric method. For the first time, we proved that L. rhamnosus GG and S. boulardii metabolites and combinations of metabolites of Saccharomycetes and lactobacteria, obtained by cultivating primary producers in their disintegrates, damage preformed 24-hour biofilms of gram-positive and gram-negative bacteria. The representatives of Corynebacterium exhibited higher sensitivity to the filtrates of disintegrates and products of vital activity of lactobacteria and Saccharomycetes than gram-negative pathogens. High parameters of decrease in optical density of preformed biofilms of Corynebacterium and antibiotic-resistant gram-negative bacteria were observed under the influence of combination of L. rhamnosus GG and S. boulardii metabolites (by 1.3–2.6 times). However, the largest reduction of the optical density of the formed biofilm of all studied strains was observed under the influence of metabolites of lactobacteria (by 1.5–5.3 times). Biologically active substances of L. rhamnosus GG and S. boulardii obtained using the author’s method can be used as candidate preparations which could have a strong influence on the process of the formation of the biofilms and preformed biofilms, and also as a preparations of substitution/addition of therapeutic prescription.
Demographic and onco-epidemiological situation in radioactive contaminated territory of Zhytomyr Oblast
Authors: Herasymchuk L. O. , Martenyuk G. M. , Valerko R. A. , Kravchuk M. M. 
Published: June 2019

We performed an assessment of demographic parameters of occurrence of malignant tumours and mortality of the population which lives in the radioactive contaminated territory of Zhytomyr Oblast (Yemilchynskyi, Luhynskyi, Narodytskyi, Korostenskyi, Olevskyi, and Ovrutskyi districts) over a 32-year period (1985–2017). The source material for the study of the demographic situation and malignant tumours in the population of the radioactive contaminated administrative districts of Zhytomyr Oblast during 1985–2017 was the statistical data of the Management of Healthcare of Zhytomyr Oblast State Administration, Central Department of Statistics in Zhytomyr Oblasts, reports on occurrences of malignant tumours of the state institution Center of Medical Statistics of the Ministry of Healthcare of Ukraine, data of the Radiological Control Service in Zhytomyr Oblast. It was determined that over 1985–2017 in the radioactive contaminated territory of Zhytomyr Oblast, a natural decline of population was observed, maximum values of which occurred in 2005 (except Narodnytsky district – 2000). The highest levels of occurrence of tumours and mortality caused by them among the adult population were observed 14 years after the Chornobyl nuclear power plant disaster. The peak of the occurrence of malignant tumours among children in the radioactive contaminated territory of Zhytomyr Oblast was observed 9 years after the explosion. A relationship was determined between doses of irradiation of the population and the risk of occurrence of malignant tumours in the radioactive contaminated regions of Zhytomyr Oblast. Taking into account that the method of calculation of passport dose (average annual effective dose of irradiation) was developed 23 years ago, nowadays it can lead to errors. This is related to change in amounts of consumption of different products by the population, which needs to be considered when determining radiological risks. The obtained data indicate that analysis of demographic and onco-epidemiological consequences of the Chornobyl catastrophe remains a relevant issue nowadays and will remain so in the near future.
Use of Carex hirta in electro-biotechnological systems on green roofs
Authors: Rusyn I. B. , Hamkalo K. R. 
Published: June 2019

Production of bioelectricity from substrates with growing plants and developing microorganisms is the newest technology of alternative energetics that has great perspectives. The efforts of scientists around the world are aimed at improving biotechnology: the development of effective electrode systems for the collection of plant-microbial bioelectricity, the search for new plants, suitable for technology, testing of new substrates for the development of plants. In this paper, we presented tests of new model electro-biosystems (EBS) consisting of graphite-zinc-steelical systems of electrodes with stainless steel elements placed in plastic containers with soil substrate and planted sedges Carex hirta. The experiment was conducted during the year on the roofs of a university building in the climatic conditions of the Western Ukrainian region to assess the functioning of the electro-biosystems in outdoor conditions. We analyzed the different types of electrode placement in containers: with the horizontal alocation of the electrodes under the root system, with the vertical placement cathodes and anodes in a container and with the increased contact area of the cathodes with the substrate and reinforced connecting of cathodes with each other. During the experiment, we monitored the bioelectric potential of the samples which were in an open circle and under load of an external resistor. To analyze short-term voltage and current, polarization measurements were performed by changing the external resistance from 10 Ω to 5 kΩ, and the current strength, current density and power density were calculated. The conducted experiments showed C. hirta can be successfully cultivated on green roofs in open soil in the climatic conditions of the Western Ukrainian region. The studied electro-biosystems operate round-the-year as the plants are frost-resistant. Metereological conditions, especially the temperature and precipitation intensity, affect the electro-performance of the electro-biosystems on the roofs. The maximum average weekly current of 21.36 mA was recorded in May at optimum temperatures and a favourable humidity level, with an average temperature of 11.4 °C and rainfall of 5.39 mm/day. The electrical performance of electro-biosystems decreases during the winter and dry periods without an organized irrigation system. During the winter period, electrode systems are damaged by adverse factors. The configuration of the electrode system EBS3 is less susceptible to breakdowns due to the destructive action of water during freezing in the winter and more effective in collecting bioelectricity. The research represented in the paper is one more step towards improving bioelectricity technology on green roofs.
Analysis of the genetic structure of a population of Lebedyn cattle by microsatellite markers
Authors: Ladyka V. І. , Khmelnychyi L. M. , Lyashenko Y. V. , Kulibaba R. O. 
Published: June 2019

Microsatellites – a separate class of molecular genetic markers, are widely used for the study of genetic variability, in particular in populations of animals bred by artificial selection under the influence of microevolutionary processes. The object of study is the gene pool of a population of animals of the Lebedyn cattle breed, which is under threat of extinction. The sample comprised 30 individuals from the farm "Komyshans'ke" in Sumy region. The analysis of population genetic structure was performed using 10 microsatellite loci recommended by FAO-ISAG: ETH225, BM2113, ETH3, BM1818, BM1824, ILSTS006, INRA023, TAGLA053, TAGLA12, ETH10. Amplification products were separated in polyacrylamide gels of different concentrations (5–8%), both native and denaturing. All studied loci were polymorphic. The number of detected alleles per locus ranged from 4 to 8 (on average 5 alleles per locus), the size of which ranged from 115 bp (ETH3) to 307 bp (ILSTS006). The majority of the investigated loci (except ETH3) belonged to valuable informative markers (PIC > 0.5). The most polymorphic TGLA053 (8 alleles), BM2113 (6) and ETH3 (6) loci have been identified. In general, the minimum number of alleles (4) was fixed in 50% loci. The main population genetic parameters for the studied loci have been calculated. The highest values of heterozygosity (He), and effective number of alleles (ne) was characterized for loci BM2113, ILSTS006, TGLA053 and ETH225. With the exception of ETH3 and VM1818 loci, the experimental group of animals is in a state of genetic equilibrium. The average value of the Wright fixation index indicates a tendency to increase in the number of homozygous individuals (inbreeding). Comparative analysis of genetic structure of breeds that have a common origin (Lebedyn (PJSC "Mykhaylivka"), Ukrainian grey (DPDG "Polyvanivka"), Red steppe (DPPR "Stepne"), etc.) has been carried out. The obtained results give grounds to assert that in the experimental population of the Lebedyn breed there are processes that lead to a decrease in genetic diversity. In order to overcome the negative effects of artificial reproduction in the gene pool of small populations of cattle, which include Lebedyn cattle, it is appropriate to use microsatellite markers in the selection and breeding work.
Impact of polyvinyl chloride, polystyrene, and polyethylene on the organism of mice
Authors: Lieshchova M. A. , Brygadyrenko V. V. , Tishkina N. M. , Gavrilin P. M. , Bohomaz A. A. 
Published: June 2019

Goods of plastic, due to their durability, universality and economical properties are broadly used in all spheres of life. On the whole, polymers are inert and nontoxic, but in the process of their production, various additives are used, which on contact or introduction into an organism has a negative effect on it. In our study, we determined the impact of some types of plastic (polyvinyl chloride, polysterene and polyethylene) on the organism of laboratory animals according to changes in their body weight, indices of mass of the internal organs, and blood parameters. For the experiment, we formed four groups of white male mice at the age of 3 weeks and average body weight of 50 g. For each group, we used different litter. For group I, the litter was sawdust; and for the other groups we added plastic products in different volumes to the sawdust; for group II finely cut polyvinyl chloride, for group III cut polyethylene, and for group IV granules of polystyrene. Every 3 days, we determined the body weight of the animals, and 32 days later we determined mass of the organs, clinical and biochemical parameters of the blood. Addition of polyvinyl chloride, polyethylene, and polystyrene into the substrate for mice did not have a significant effect on tempi of growth of body weight, and also relative mass of heart and lungs. Polyvinyl chloride and polystyrene have an immune-suppressive effect, and polyvinyl chloride affects both central and peripheral organs, and polystyrene mostly harms the peripheral organs. All used types of plastic cause leukocytopenia, following which neutrophilia of band neutrophils and monocytosis takes place as a result of damage to the biological barriers. We determined the systemic toxic effect of the studied types of plastic on the internal organs, which manifested in increase in their mass (liver, kidneys), steep increase in the activity of liver enzymes (AST, ALT), simultaneous decrease in activity of alkaline phosphatase and content of cholysterol and glucose in the blood serum of the mice. Also polyvinyl chlorine, polyethylene and polystyrene cause degeneration of the epithelium of the uriniferous tubule, which is manifested in reduction of globulins and creatinine in the blood of animals from the experimental groups following increase in relative mass of the kidneys. The results of our research allow us to state that different types of plastic can cause toxic effect on animals, as well as people who are in frequent contact with them.
Differentiation of maize breeding samples by β-carotene content
Authors: Satarova T. M. , Semenova V. V. , Zhang J. , Jin H. , Dzubetskii B. V. , Cherchel V. Y. 
Published: June 2019

Plant carotenoids are important micronutrients in the diet of humans and animals, since they act as precursors for the synthesis of vitamin A in animal cells. The most effective precursor to the vitamin A biosynthesis is β-carotene. Increasing the β-carotene content in maize grain as an important feed and food crop is an urgent task for plant selection. The purpose of this work was to differentiate maize breeding samples from the Dnipro breeding program by the β-carotene content in mature grain. Maize grain of 18 inbreds harvested in 2015 and 5 inbreds harvested in 2016 was researched. Determination of β-carotene content in matured dry grain was carried out after petroleum ether extraction and ultra performance liquid chromatography (UPLC) in the mobile phase of methanol/acetonitrile. The β-carotene content in the grain of genotypes from the Dnipro breeding program was on average 1.020 mg/kg for inbreds grown in 2015, and 0.672 mg/kg for inbreds grown in 2016. These values correspond to the β-carotene content in the grain of the majority of genotypes from world breeding programs selected by methods of classical selection. The inbred DKV3262 with white grain had the smallest content of β-carotene (0.076 mg/kg), while the yellow-coloured line DKD9066 had the highest one (2.146 mg/kg). The variation in the grain β-carotene content in different years of maize cultivation was noted. Inbreds of flint and semident maize showed the general tendency to increase the β-carotene content in grain compared with dent ones. The distribution of the studied inbreds on germplasm types showed the significant variation of β-carotene content in grain and the incidence of relatively high values in all germplasms analyzed. Inbreds containing more than 1.5 mg of β-carotene per 1 kg of grain, DK239, DK206A, DK212, DKD9066 and DKE-1, are emphasized as promising for the selection to increase the content of valuable micronutrients.
Pathomorphological changes in the large intestine of rabbits parasitised by Passalurus ambiguus (Nematoda, Oxyuridae)
Authors: Mykhailiutenko S. M. , Kruchynenko O. V. , Klymenko O. S. , Serdioucov J. K. , Dmytrenko N. I. , Tkachenko V. V. 
Published: June 2019

Passaluriasis is a common infestation of lagomorphs, which clinically manifests in intense itching around the anus, various disorders of digestion and loss of weight. We performed a study of infested rabbits of the seryi veleten breed, which were kept in individual farms in Poltava Oblast (Ukraine). During the autopsy, in the cavity of the large intestine, there were found small, spindle-like helminths of white colour. Out of 10 rabbits, 846 nematodes were extracted (369 males and 477 females, identified as Passalurus ambiguus (Rudolphi, 1819) Dujardin, 1845. It was determined that the extensity of infestation in female rabbits reached 56.4%, male rabbits – 43.6%. The results of metric parameters of the nematodes are as follows: mean body length of males equaled 4.6 ± 0.4, females – 9.7 ± 1.21 mm, and maximum width – 257.4 ± 17.8 and 546.2 ± 37.1 µm respectively. A common feature of mature nematodes of this species is the presence of a rounded extension (bulbus) at the end of the esophagus. During chronic course of passaluriasis in rabbits, pathomorphological changes developed in the large intestine. The upper epithelium of the large intestine was damaged in some places, certain epithelian cells were found in the lumen of the intestine. The intestinal glands are widened, filled with slimy content of mostly dark blue-violet colour, especially in the upper sections of the glands. In the deep sections of the glands, slime was almost transparent. In deep sections of the glands the slime was almost transparent. In the epithelium cells of the glands, slimy granules were also of basophilic colour. The muscularis mucosae of the mucous membrane, especially between the intestinal glands was swollen, had practically no colour, the collagen fibers were stretched, thinned-out. Edemas were also recorded in the tela submucosa. In some preparations, the muscularis mucosae was notably infiltrated by lymphoid cells and histiocytes. On large extensions, we found some signs of hypersecretion of slime in goblet cells of the intestinal glands as accumulations of granules of slime in the cytoplasm, which had poorly developed colouration. On the side of the muscular and serous membranes, no pathological changes were found. The information presented here indicates the relevance of passaluriasis infestation as an etiological factor of occurrence of chronic catarrhal colitis with lymphohistiocytosis.
Synergistic and antagonistic effects of insecticide binary mixtures against house flies (Musca domestica)
Authors: Levchenko M. A. , Silivanova E. A. 
Published: June 2019

The house fly, Musca domestica Linnaeus, 1758 (Diptera, Muscidae), is known as a globally distributed parasite with veterinary and medical importance and the ability to develop resistance to insecticides Insecticide mixtures can contribute to enhancing the effectiveness of existing insecticides against house flies and to implementing insecticide resistance management. The present study was conducted to assess the efficacy of four insecticides with different modes of action, applied alone and in binary mixtures, against adults of the M. domestica laboratory strain by no-choice feeding bioassays. The interaction patterns of neonicotinoid acetamiprid, phenylpyrazole fipronil, avermectin ivermectin, and pyrrole chlorfenapyr in the binary mixtures were likewise analyzed by calculating the combination indices to find out combinations with the synergistic effect. The analysis of values of insecticide lethal concentrations for 50% mortality revealed that the toxicity of acetamiprid, fipronil, and ivermectin increased in the binary mixtures compared to when they applied alone, while the toxicity of chlorfenapyr depended on the second insecticide in the mixtures. The combination index values of five insecticide mixtures, fipronil/acetamiprid (1:10), fipronil/chlorfenapyr (1:4), ivermectin/acetamiprid (1:2.5), ivermectin/chlorfenapyr (1:3 and 1:10) were <1, which displays a synergism. Three insecticide mixtures, acetamiprid/chlorfenapyr (1:4), fipronil/ivermectin (1:4), fipronil/chlorfenapyr (1:40), had combination index values >1, which indicates an antagonism. The fipronil/chlorfenapyr (1:4) mixture was the more toxic to adults of M. domestica. The ivermectin/chlorfenapyr (1:10) mixture and the ivermectin/acetamiprid (1:2.5) mixture produced the highest synergistic effects. The results of the present study suggest that the interaction patterns (synergistic or antagonistic) in the insecticide mixtures can depend on both the combination of insecticides and their ratio. Further studies are required in order to evaluate the synergistic combinations against field populations of M. domestica.