bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet Aggregometry and Shape Change Measurement by Laser Light in Small Samples
Authors: Blasberg P. , Wurzinper L.J. 
Published: May 2019

A technique is presented to measure platelet aggregation (PA) and platelet shape change (PSC) with high accuracy in small samples (100 ul) using a turbidimetric method wich a He-Ne-Laser as light source. Both, transmitted and scattered light, Is measured by two independent light sensitive elements. Transmitted light is detected by a photodiode with a sensitive area of 1 mm2 covering a space angle of Ω= 0.004 sterad. Scattered light is detected by a photoelement surrounding the photodiode which covers a space angle of U-1.4 sterad. Ar, electronic set up forms a signal, which is proportional to the ratio scattered light/scattered light + transmitted light.The output voltage is linearly correlated to the turbidity of the suspension. The whole set up is employed with three different types of cuvettes.1. A cuvette where through a semipermeable hollow fiber membrane different drugs can diffuse into the platelet suspensions avoiding any stirring of the sample ()2. A flow through cuvette to measure the extent of PA in PRP samples.3. A couette type platelet aggregometer.
Aggregation and Disaggregation Examined by Computerized Platelet Aggregation Analysis
Authors: Dayhoff R.E. , Zeller J.A. , Ledley R.S. , Rotolo L.S. 
Published: May 2019

Computerized Platelet Aggregation Analysis (CPAA) quantitates free platelets and aggregates found in p.r.p. Miltiple microscopic fields of thousands of platelets are viewed and the percentage of platelets in discrete aggregate size groups is determined. Aggregation and disaggregation have been studied by CPAA (1) before, (2) during, and (3) after stimulation with ADP concentrations that produce modest, brief, and apparently totally reversible aggregation by light transmission (LT) acraregcmetry, as seen in the table.The five normal male subjects studied all showed aggregation by CPAA, but not by LT aggregemetry, before and 10 min. after ADP stimulation. CPAA indicated, in 4 of 5 subjects that disaggregation was incomplete at 10 min, (3), as compared to before ADP (1) or lu mm. without ADP (4), CPAA demonstrated aggregation prior to ADP stimulation and showed that small changes of LT (4%) are accompanied by a loss of about 36% of the free platelets Lo aggregates, seme greater than 40 platelets in size. Both aggregation and disaggregation are quantitated by CPAA which provides information not otherwise obtainable.
Prostaglandins and Platelet Function in Diabetes
Authors: García-Conde J. , Amado J.A. , Merino J. , Benet I. 
Published: May 2019

We have studied platelet aggregation induced by 0,5 mM. Araquidonic acid (AA) addition to platelet-rich-plasma (PRP) from 21 insulin treated diabetic patients and in 21 non-diabetic controls. The velocity of aggregation was significantly higher in the diabetic group. There was no differences in the velocity of aggregation in patients with or without retinopathy.The incubation of PRP of normal subjects at 37- during 5 minutes with 5,8 10-4 M. Imidazole changed the pattern of aggregation: The velocity of aggregation was slower and appeared a wave of disaggregation. Imida zole had not effect on aggregation in the diabetic group. This data add support to the findings published by COLWLLL showing that platelets from diabetics have hyperactive AA metabolism. Prostaglandin I2 (PGI2) obtained from rat aorta shows an inhibitory effect on ADP or AA induced aggregation. This effect is less marked in diabetic PRP than in PRP of normal controls. PGI2 release in platelet-poor-plasma from diabetics is normal. This can represent a resistance ot diabetic platelet to the anti aggregating effect of PGI 2. A similar finding was also appreciated with the PGE1 in three out of six patients so tar stu
Hyperviscosity and Coagulation Activation in Peripheral Vascular Disease
Authors: Stuart J. , George A.J. , Blunt R. J. , Strachan C.J.L. , Hurlow R.A. 
Published: May 2019

Forty-two patients with Raynaud’s disease (21 idiopathic, 8 scleroderma, and 13 industrial) and 18 patients with lower limb vascular disease were matched for age, sex and smoking habit with 50 healthy controls. Whole-blood viscos ity was measured at 37, 27 and 220 and at 128, 23 and 0.2 s-1. Tests of platelet activation, coagulation, and fibrinolysis were also performed. All blood was taken understandardised, fasting, out-patient conditions.Patients with extensive lower limb atherosclerosis showed an acute phase protein reaction (raised fibrinogen, factor VIII and haptoglobins), decreased fibrinolysis, and hyperviscosity. Patients with the milder forms of Raynaud’s disease showed hyperiscosity only, with normal or increased fibrinolysis, whereas patients with vibration induced disease showed more extensive changes. A minor degree of platelet activation occurred in all groups.The differences in platelet function, coagulation, fibrinolysis, and vis cosity between these vascular disorders probably reflect the extent of endothelial cell damage irrespective of its aetiology.
Platelet Release Reaction - A Predictor of Death Following Myocardial Infarction?
Authors: Heptinstall S. , Mulley G.P. , Taylor Patricia M. , Mitchell J.R.A. 
Published: May 2019

A comparison has been rrade of the extent to which ADP can induoe the release reaction in platelets from 26 patients who, within 5 days, had sustained a myocardial infarction and fran 54 age-rratched arrbulant control subjects. Platelet rich plasma was prepared from blood collected into heparin (10 u/ml), samples were incubated with 3H-serotonin., aggregation was then induced by adding ADP (10 μM, final concentration) and the extent to which ADP irrluced the release reaction was detennined by rreasuring the arrount of the accumulated 3H-serotonin that was released.The results ootained for the group of patients who had experienced a myocardial infarction were rot significantly different from those obtained for the ccntrols. However, a significant difference (P = 0.02) was observed between the results obtained for those patients who died within 12 months of the infarction (mean 29.4%± 5.6 of accumulated 3H-serotonin released by ADP) and those who survived (mean 12.0%±3.3). Of the 8 patients who died, 7 had platelets that released ≥ 20% of the 3H-serotonin that had platelets of the 18 patients who survived only 4 had platelels that released ≥ 20% 3H rotonin.
Three Sources of Purified Factor III for Monitoring Vitamin K Dependent Coagulant Factors Using a Synthetic Chromogenic Substrate
Authors: Oostryck R. 
Published: May 2019

Methods used for monitoring coumarol treatment should be sensitive to the loss of calcium binding γ-carboxyl glutamic acids in the vitamin K dependent clotting factors. A two stage method sensitive to factors II-VII-X using the chromogenic substrate H-D-Phe-Pip-Arg-pNA has been evaluated using 75μl of reconstituted factor III containing 0.012μg of apoprotein III per ml for each assay. The reconstituted factor III used as the activator was prepared according to the method of Bjorklid et al. (1973) from human brain, human placental material and bovine brain, and from a phospholipid fraction - Gierksky et al. (1976).This method was sensitive to the coumarol induced defect with all three sources of apoprotein III and correlated well with a standard saline thrombo plastin extract (r=0.85, 0.89 and 0.88 respectively) in a group of 80 dicouroarol treated patients and 20 normal subjects and appears to overcome the insensitivity usually associated with crude bovine thromboplastins. Bjorklid E, Storm E and Prydz H (1973)Biochemical and Biophysical Research Communication, 55, 969
Gierksky K E, Bjorklid E and Prydz H (1976) Scandinavian Journal of Haematology,16, 300.
Vertebrates Coagulation: the Intrinsic and Gxtrinsic Pathways; Extraphysiological Activators
Authors: Dange M. , Leroy J. , Degenne M. , Leclerc M.H , Theron J.F , Delahousse B. 
Published: May 2019

A systematic coagulation study of forty different species of vertebrates using autoreagents (i.e: prepared from their own tissues), revealed biological activity of factors thought till now to be absent so early in the phylogenetic evolution because of their unreactivity with respect to standard reagents used currently in man.Hybrid plasma fractions from man and other animal species demonstrated that the best extrinsic pathway kinetics were obtained by associating specific thromboplastins and specific factors VII-X.The comparison of extraphysiological coagulation activators (such as Russell viper venom, Echis carinatus viper venom, staphylocoagulase, thrombin coagulase, Bothrops atrox viper venom, Agkistrodon contortrix snake venom) permits a chronological approximation of the coagulation factors evolutionary variations.It should be noted that the Russell viper venom pathway is found in all vertebrates.
Effect of Age and Site on Transfusion Requirements of Adolescents With Severe Haemophilia A.
Authors: Aronstam A. , McLellan D.S. , Turk P. 
Published: May 2019

4935 bleeding episodes in 75 adolescents with severe haemophilia A and without inhibitors to Factor VIII were studied during 5 years 1973-1977 6726 Factor VIII transfusions were given. 1.22 transfusions per bleed given in 1973 rose to 1.39 transfusions per bleed in 1977. 1.20 transfusions per bleed were given to 10 year olds which rose to 1.47 at 15 years. 745 bleeding episodes needed retransfusion within 24 hours. Iliopsoas (61% of bleeds) and retroperitoneal (58% of bleeds) were the sites needing most retransfusions wichin 24 hours. Total transfusions per bleed were most frequent in retroperitoneal (3.6) and iliopsoas (2.5) bleeds. On 181 occasions bleeds occurred into more than one site simultaneously and required 221 transfusions for complete resolution. Multiple bleeds involving the thigh (1.4) and upper arm (1.4) required most transfusions per bleeding episode.Conclusions are that adolescent transfusion requirements peak at 15 years. Bleeds into the psoas and retroperitoneum may benefit from higher initial doses of Factor VIII and multiple bleeds require no more initial therapy than single bleeds.
Platelet Regeneration time in Cardiovascular Patients
Authors: Roncucci R. , Deperon R. , Destailleur J. , Doumont J. , Lambelin G. , Larson J. , Stalle Van F. , Verlegne R. 
Published: May 2019

Platelet regeneration time (PRT). based on the assessment of platelet malondialdehyde (MDA) production before and repeatly after intake of one single dose of acetylsalicylic acid (ASA). has been determined using a sensitive and semi-automated method in healthy young volunteers. old healthy subjects. patients with vascular prosthetic material and patients with occlusive vascular disease (1). The mean PRT t/2 for 9 healthy young volunteers was found to be 4.0±0.3 days. In 15 patients who had undergone either heart valve replacement surgery or aortoiliac reconstruction the mean PRT t/2 was drilmatically shorter (2.40±0.1 days; p < .001). In the group of patients with occlusive vascular disease (n = 13). the mean PRT t/2 was also shorter but the difference was not statistically significant (3.4±0.3 days; p > .05); it must however be noted that six of these patients showed a shortened PRT t/2 value comparable to that observed in patients with vascular prosthetic material. Moreover. investigations designed to compare PRT in healthy young volunteers and in old heal thy subjects suggest that age does not affect per se PRT.(1) R. Roncucci et al., Thromb .Res., accepted for publication.
Platelet Aggregation Related to age in Diabetes Mellitus
Authors: Lecrubier C. , Scarabin P.Y. , Grauso F. , Samama H. 
Published: May 2019

The relation between age and platelet sensitivity to various aggregating agents has been little explored until now. Previous studies have essentially concerned normal controls.Platelet aggregation induced by ADP and collagen was studied in 174 diabetic patients before entering a controlled clinical trial.The clinical characteristics have been precisely defined. The degree of retinal abnormality was assessed by fundus angiofluorography in every patient. Most patients had background retinopathy ; the remainder were normal. All results were computer analysed. The only significant relationship observed was between platelet sensitivity to ADP which increases in relation regularly to age in both insulin and non insulin treated diabetic patient with or without background retinopathy.Spontaneous platelet aggregation was observed in 13 subjects and was not correlated to the existence of a neuropathy or any other clinical characteristics.
Speckle-Tracking-Echokardiografie – ein neues Tool für die Intensivstation?
Authors: Reckefuß Norbert , Heuer Jan , Butz Thomas 
Published: May 2019

ZusammenfassungDie Beurteilung der kardialen Funktion kritisch kranker Patienten ist eine wichtige diagnostische Maßnahme auf Intensivstationen. Jedoch kann die meist angewandte detaillierte echokardiografische Untersuchung gerade Ungeübte rasch an ihre Grenzen bringen. Zusätzliche Informationen vermag die myokardiale Deformationsanalyse mittels Speckle-Tracking-Echokardiografie zu liefern – ein innovatives Verfahren, das in diesem Beitrag vorgestellt wird.
Antioxidant potency of various mangosteen (Garcinia mangostana,L) pericarp extract fractions on HbA1c and fasting blood glucose level in diabetic mice
Authors: Husen S A , Hayaza S , Susilo J K , Setyawan F , Zuraidah A A , Winarni D , Darmanto W 
Published: May 2019

Abstract
This study is purposed to explore the antioxidant potency of various mangosteen (Garcinia mangostana L) pericarp extract fractions on HbA1c and fasting blood glucose level in diabetic mice. Study conducted using 3-4 months old male mice as models. Animal models split into 2 main groups; non-STZ normal control group and STZ-treated diabetic mice. STZ induced via multiple low dose method of 30 mg/kg BW administered for 5 days (given daily). Diabetic mice group then divided into 3 subgroups, which were diabetic control group, Metformin-HCl diabetic group, and treatment group given with various mangosteen pericarp extract fractions. Treatment group separated into 3 groups based on various fraction administered; 18 mg/kg BW non-polar (NP) fraction, 80 mg/kg BW semi-polar (SP) fraction, and 50 mg/kg BW polar (P) fraction. All fraction dose equals to 2.5 gram/kg BW of powdered mangosteen pericarp extract dry weight. Control group and various extract fractions treated for 14 days. Body weight, HbA1c, and fasting blood glucose level (after mice fasted for 5-6 hours) measured on 15th day. Result shows significant differences on HbA1c and fasting blood glucose level of treatment group compared to control group, specifically non-polar and polar fraction treatment significantly reduce HbA1c and fasting blood glucose level of diabetic mice.
Phytoremediation of domestic wastewater (detergent) with arrowhead and burhead plants in Purwodadi Botanic Garden
Authors: Adistiara V Y , Kustiyaningsih E , Irawanto R 
Published: May 2019

Abstract
The aquatic plants is use for reduce the organic materials levels contained in the domestic wastewater. Some aquatic plants can be used as a phytoremediator in an effort to overcome water pollution, one of them is Arrowhead (Sagittaria lancifolia) and Burhead (Echinodorus radicans). The aim of this research is to know how Arrowhead and Burhead plants as phytoremediation of domestic wastewater (detergent) in Purwodadi Botanic Garden. The design of this study was Randomized Block Design (RAK) with 2 factors: crop treatment on medium and locus of domestic wastewater concentration (detergent) 0 g/L (as control); 0,05 g/L; 0,1 g/L; 0,15 g/L. The data observation are plant morphology, plant water level, plant biomass at the beginning and end of environment and environmental parameters such as temperature and humidity. The results obtained were no significant morphology of Echinodorus radicans plants observed in green and fresh colours, for water levels of 0,05 g/L, decreased by 0,5 cm, concentrations of 0,1 g/L down 0,6 cm, concentration 0,15 g/L down 1 cm. At the observation of biomass difference of 0,05 g/L concentration of 3,6 g; 0,1 g/L concentration of 5,6 g; 0,15 g/L concentration of 8,6 g. On observation the average temperature is 22-25°C and the moisture average 52-64%. The results obtained were for morphology Sagittaria lancifolia mortality rate, for water level with concentration 0,05 g/L, amount 0,4 cm; concentration 0,1 g/L or decreased 0,3 cm; concentration 0,15 g/L decreased 0,4 cm. At the observation of biomass difference of 0,05 g/L concentration of 5,5 g; 0,1 g/L concentration of 3,5 g; 0,15 g/L concentration of 39,7 g. On observation the average temperature is 22-25°C and the average moisture averages 60-64%.
Heavy metals (Cd, Pb, Cu, Zn) concentrations in edible bivalves harvested from Northern Coast of Central Java, Indonesia
Authors: Yulianto B , Oetari P S , Februhardi S , Putranto T W C , Soegianto A 
Published: May 2019

Abstract
This study measured the concentration of Cd, Pb, Cu and Zn in bivalves captured from the northern coast of Central Java, Indonesia. We also evaluated the hazard level posed by the metals in relation to the maximum acceptable limit (MAL) for human consumption and to estimate the weekly intake and compare it with the provisional tolerable weekly intake. The highest Cd levels were recorded in Amusium pleuronectes (16.32 mg Cd/kg) and the lowest levels of Cd were observed in Perna viridis (0.18 mg Cd/kg). A. granosa contained the highest level of Pb (9.050 mg Pb/kg) and P. viridis contained the lowest level of Pb (1.07mg Pb/kg). The lowest Cu level was observed in A. granosa (1.49 mg Cu/kg) and the highest Cu level was noted in C. gigas (86.21 mg Cu/kg). The levels of Zn in all species were relatively higher compared to other metals, ranged from 33.56 to 846.81 mg Zn/kg. The Cd level in bivalves was below the MAL of WHO and FAO (2 mg/kg), except in A. pleuronectes from Tegal city. The level of Pb in bivalves was exceeded the MAL value of the various existing authorities. Cu level in C. gigas (59.2 mg Cu/kg) exceeded the MAL of Indonesian authority and WHO. Zn levels in C. gigas, A. pleuronectes and A. granosa exceeded the MAL of various authorities. In order to avoid the impact of metals on people health, then safely weight of bivalves were allowed to be consumed were 0.10 kg/week for A. granosa, 0.10 kg/week for C. gigas, 0.16 kg/week for M. lyrata, 0.36 kg.week for P. viridis and 0.03 kg/week for A. pleuronectes.
Role of Dracaena marginata plants as absorbent of lead (Pb) pollutants in city park of Surabaya: a preliminary study
Authors: Hamidah  , Farli D P 
Published: May 2019

Abstract
Plants Dracaena marginata is a plant that is often used as a plant landscape in the city Park of Surabaya. Dracaena marginata plant is expected to absorb well air pollutants. Air pollutants come from motor vehicles.One of the harmful pollutants that can be absorbed by plants is heavy metal lead (Pb).The aim of this research is to know the role of Dracaena marginata which acts as a landscape plant and its role as absorbant of Pb metal pollutant. The aim of this research is to know the role of Dracaena marginata which acts as a landscape plant and its role as absorbent of Pb metal pollutant. The method used in this research is observational, that is identifying, measuring density and descriptive plant Dracaena marginata. The study sample was taken in two places, namely the city park which is near the big road (lots of pole) and in the park which is small, in the city parks in Surabaya. It also measured the content of Pb metal on leaves by Atomic Absorption Spectrophotometry (AAS) method. From the research results obtained data that found two varieties Dracaena marginata, namely, varieties of green and red. Dracaena marginata is a good plant to absorb Pb pollutants. Dracaena marginata can absorb many Pb metals, but the growth and development of the body is not disturbed (grows well). The conclusions of this study are (1) there are two kinds of Dracaena marginata green var and Dracaena marginata red var (2) Dracaena marginata is a bioindicator can absorb many metal Pb.
Minimizing failure of production quality from refill drinking water in Gubeng District, Surabaya City, using failure mode and effect analysis
Authors: Wahyuningsih I , Karnaningroem N 
Published: May 2019

Abstract
Refill water is one important element in filling the increasing drinking water demand. The price offered by refill water depot is significantly lower than packaged water, however, its quality does not meet the standards, and therefore it will potentially affect people’s health. This risk surfaces if refill water depot installation can’t treat raw water as well as the environment surrounding properly. Thus, identification to refill water production failure cause is needed to minimize the possibility of failure happening. Identification and risk analysis processes are based on risk management concept by using Failure Mode and Effect Analysis (FMEA). Failure cause identification is done using fishbone diagram to find the root of the problem causing the failure. The root of the failure will then be given score to determine the biggest risk stated in Risk Priority Number (RPN). RPN value is obtained through multiplying severity, occurrence, and detection. The biggest failure cause in refill water depot is in the disinfection technology used, which is the replacing and contact duration of regulation regarding refill water also causes failure in refill water production process.
Minimizing failure risk of refill drinking water production in Rungkut district Surabaya using Failure Mode and Effect Analysis (FMEA)
Authors: Wicaksono A , Karnaningroem N 
Published: May 2019

Abstract
Human necessity toward consumable drinking water increases along with population growth. People nowadays have a hard time obtaining clean water because of polluted river. To meet daily necessity of drinking water, a lot of industries open drinking water business, particularly refill drinking water facility (DAMIU). The utilization of refill drinking water facility has its own hazardous risk because of the doubtful quality, moreover if the customers aren’t paying attention towards the security and hygiene. This study used FMEA (Failure Mode Effect and Analysis) to determine the priority of failure incident in refill drinking water treatment process. This FMEA method was used because FMEA has advantage over other method in term of being more feasible and effective for operational improvement while being able to analyze risk in bigger and more complex scale. To determine the failure, we first did risk identification which contained failures information obtained from interview, quality test, and direct observation. Failure causes risk happened in refill drinking water facilities were analyzed using Fishbone method. The location of the study was in Rungkut District, Surabaya. The parameters used were turbidity, TDS, pH, and Total Coliform. Obtained risk should had a value to be measured and determined for the handling priority and to find improvement effort. The measurement value was called as RPN (Risk Priority Number) which was the multiply value of severity, occurrence, and detection. Based on analysis result using fishbone method there were 4 factors which caused failure for total coliform parameter. Then, it was analyzed using FMEA method, and it was found that failure happened within 3 highest priority, which was UV system contact duration, ozone system contact duration, and disinfection tool replacement and maintenance.