bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metal trabecular bone reconstruction system better improves clinical efficacy and biomechanical repair of osteonecrosis of the femoral head than free vascularized fibular graft: A case–control study
Authors: Chen Xian‐Tao , Zhu Ying‐Jie , Liu You‐Wen , Chen Ke , Xu Wen‐Wen , Zhang Lei‐Lei , Liang Da‐Wei , Li Jing , Ye Ye , Tian Ke‐Wei , Zhang Xiao‐Dong , Li Hong‐Jun , Kang Zhen 
Published: May 2019

AbstractIn this study, we aim to compare and analyze the biomechanical repair and clinical efficacy of osteonecrosis of the femoral head (ONFH) with the use of metal trabecular bone reconstruction system and free vascularized fibular graft. The study enrolled 66 adult patients from medical records of nontraumatic ARCO 2A–3B stage ONFH. A simple ONFH model without surgical treatment was established in 13 cases, 29 cases were treated with metal trabecular bone reconstruction system, and 24 cases were treated with free vascularized fibular graft. Computer‐recognized and extracted femur outlines were imported, and three‐dimensional reconstructions were performed. The stress concentration and stress peak value were analyzed, and the Harris score, visual analog scale pain score, and operation status of the above patients were compared. Finally, quality of life assessment was performed using SF‐36 scale. Metal trabecular bone reconstruction system provided less operation time, blood loss, and the total length of postoperative hospital stay than free vascularized fibular graft. Metal trabecular bone reconstruction system promoted bone reconstruction, increased bone mineral density and Harris score. The total clinical effective rate of young patients (20–40 years) was higher than that of older patients (41–60 years). Metal trabecular bone reconstruction system provided higher physical component summary, mental component summary, and role/social component summary than free vascularized fibular graft. This study demonstrates that both metal trabecular bone reconstruction system and free vascularized fibular graft can prevent or delay the progression of ONFH, while metal trabecular bone reconstruction system is a better choice because of better short‐term clinical efficacy.
Effect of nucleolin on adriamycin resistance via the regulation of B‐cell lymphoma 2 expression in Burkitt's lymphoma cells
Authors: Mei Xuqiao , Chen Yanxin , Gan Donghui , Chen Yingyu , Wang Lingyan , Cao Yanqin , Wu Zhengjun , Liu Weijuan , Zhao Chenxing , Lin Minhui , Yang Ting , Hu Jianda 
Published: May 2019

AbstractNucleolin (NCL, C23) is an important nucleocytoplasmic multifunctional protein. Due to its multifaceted profile and high expression in cancer, NCL is considered to be a marker of drug resistance associated with chemotherapy. However, the biochemical mechanisms in which NCL suppresses drug sensitivity in several cancers have yet to be fully elucidated. This study aims to explore the effect of NCL on drug sensitivity and its potential mechanism in CA46 Burkitt's lymphoma (BL) cells. CA46 BL cells were transfected with lentiviruses carrying the NCL gene (CA46‐NCL‐overexpression, CA46‐NCL‐OE), or shRNA sequences that target the endogenous NCL gene (CA46‐NCL‐knockdown, CA46‐NCL‐KD). Adriamycin (ADM) IC50 levels for CA46‐NCL‐overexpressed (OE), CA46‐NCL‐OE control (OEC), CA46‐NCL‐knockdown (KD), and CA46‐NCL‐KD control (KDC) cells were 0.68 ± 0.06 μg/ml, 0.68 ± 0.06 μg/ml, 0.68 ± 0.06 μg/ml, and 0.30 ± 0.04 μg/ml, respectively. Apoptosis rates were significantly increased following NCL KD, whereas the opposite effect was noted in OE cells. A significant reduction of B‐cell lymphoma 2 (Bcl‐2) mRNA and protein levels in KD cells was observed, while OE cells displayed the opposite effect. The stability of Bcl‐2 mRNA was influenced by NCL levels, the half‐life of which was extended after NCL‐OE, whereas it was reduced in KD cells. Finally, results of RNA‐immunoprecipitation assays indicated that NCL could bind to Bcl‐2 mRNA in CA46 cells. Taken together, these results suggested that NCL could mediate Bcl‐2 expression and stability, and thus enhance ADM resistance in CA46 BL cells.
Scutellarin inhibits hypoxia‐induced epithelial‐mesenchymal transition in bladder cancer cells
Authors: Lv Wei‐Ling , Liu Qian , An Ji‐Hong , Song Xiao‐Yong 
Published: May 2019

AbstractScutellarin, an active component of flavonoid, displays a variety of physiological actions and has been applied for the treatment of diverse diseases including hypertension and cerebral infarction as well as cerebral thrombosis. In recent time, Scutellarin has been demonstrated to possess the anticancer activity. But the biological significance of Scutellarin in bladder cancer (BC) remains to be elucidated. In the current study, we explored the specific effect of Scutellarin on BC progression. We found that Scutellarin inhibited hypoxia‐induced BC cell migration and invasion in vitro as well as suppressed hypoxia‐induced BC metastasis in vivo. Moreover, Scutellarin significantly reversed hypoxia‐promoted epithelial‐mesenchymal transition (EMT) in BC cells and the PI3K/Akt and MAPK pathways were implicated in the suppressive effect. Taken together, we suggested the potential value of Scutellarin as a novel anticancer agent for BC treatment.
Primary cardiac lymphoma in both atria: A case report
Authors: Li Yingtao , Zhou Zhenfeng , Xin Fangjie , Zhang Chi , Zhang Rong , Sun Dongmei , Liu Weigang 
Published: May 2019

AbstractPrimary cardiac lymphoma (PCL) is a very rare kind of primary heart tumor, belonging to non‐Hodgkin's lymphoma whose major pathological type is diffuse large B‐cell lymphoma. The common symptoms include heart failure, pericardial effusion, and malignant arrhythmias. Early diagnosis and treatment of PCL are of great importance due to its merely 7‐month median survival period. Here, we report a 48‐year‐old male patient in whom both atria were involved and had to undergo palliative surgery because of severe clinical symptoms.
Treating lysosomal storage disorders: What have we learnt?
Authors: Lachmann Robin H. 
Published: May 2019

AbstractThe first enzyme replacement therapy (ERT) for a lysosomal storage disorder (LSD) was approved in 1991 and we now have more than 25 years of experience of treating patients with type 1 Gaucher disease. Because of the remarkable success of this therapy, enormous effort and resource has gone into developing other ERTs, for Gaucher (where three different enzyme preparations have now been approved) and for other LSDs. We now have more than 10 years of clinical experience in using ERT to treat Gaucher, Fabry, Pompe and MPS I, II, and VI. This article aims to assess the real‐life experience of a selection of these innovative and expensive treatments to see if they have met the high expectations which were set for them when they launched.
Treasure hunt for peptides with undefined chemical modifications: Proteomics identification of differential albumin adducts of 2‐nitroimidazole‐indocyanine green in hypoxic tumor
Authors: Wang Lei , Dietz Christopher , Zhou Feifei , Erfanzadeh Mohsen , Zhu Quing , Smith Michael B. , Yao Xudong 
Published: May 2019

Abstract2‐Nitroimidazole is a well‐known chemical probe targeting hypoxic environments of solid tumors, and its derivatives are widely used as imaging agents to investigate tissue and tumor hypoxia. However, the underlying chemistry for the hypoxia‐detection capability of 2‐nitroimidazole is still unclear. In this study, we deployed a biotin conjugate of 2‐nitroimidazole‐indocyanine green (2‐nitro‐ICG) for the investigation of in vivo hypoxia‐probing mechanism of 2‐nitro‐ICG compounds. By implementing mass spectrometry‐based proteomics and exhaustive data mining, we report that 2‐nitro‐ICG and its fragments modify mouse serum albumin as the primary protein target but at two structurally distinct sites and possibly via two different mechanisms. The identification of probe‐modified peptides not only contributes to the understanding of the in vivo metabolism of 2‐nitroimidazole compounds but also demonstrates a competent analytical workflow that enables the search for peptides with undefined modifications in complex proteome digests.
Diagnostic accuracy and adherence to treatment guidelines in adult inpatients with urinary tract infections in a tertiary hospital
Authors: Chen Annie K. D. , Duffy Eamon J. , Ritchie Stephen R. , Thomas Mark G. 
Published: May 2019

AbstractBackgroundAntibiotic prescribing for patients with urinary tract infections (UTIs) is often inconsistent with published guidelines. Pharmacists have a key role in the multidisciplinary team to improve the prescribing of antibiotics.AimThis study determined the reasons for low guideline adherence and investigated the effects of a pharmacist‐led, non‐confrontational, inexpensive educational intervention on guideline adherence in adult inpatients with UTIs.MethodsThe study was performed in three general medical wards in Auckland City Hospital (ACH), New Zealand, during March–September 2015. All patients treated for UTIs were identified by ward pharmacists. A pre‐intervention observation period was followed by an intervention period in which ward pharmacists placed a simple diagnostic and treatment algorithm in the clinical records of all patients being treated for a UTI. Clinical and laboratory data available at the time initial antibiotic therapy was prescribed were used to determine a retrospective diagnosis for each patient. The initial antibiotic therapy was evaluated for adherence to the ACH antibiotic guidelines in relation to both the prescriber's and retrospective diagnosis.ResultsOverall, the prescriber's diagnosis was concordant with the retrospective diagnosis in only 48 of 141 episodes (34%). Treatment was adherent to guidelines in 27% pre‐intervention and 33% intervention episodes (p = 0.5).ConclusionInaccurate clinical diagnosis in patients with UTIs was a common reason for antibiotic treatment to be non‐adherent to guidelines. The study failed to find an association between the educational intervention and an effect on guideline adherence. Passive interventions are unlikely to be effective and further interventions should consider codesign with users.
Treatment of sleep disturbance in older adults
Authors: Reynolds Amy C. , Adams Robert J. 
Published: May 2019

AbstractSleep and circadian rhythm disturbances are prevalent in older populations. Numerous factors can affect sleep, including medications, living environment and physical, mental and psychosocial factors (e.g. loneliness and stress). Sleep architecture changes with aging, with less deep sleep (slow wave sleep) and an increased frequency of awakenings after sleep onset. Benzodiazepines, Z‐drugs and low doses of the tricyclic antidepressant doxepin have modest positive benefits for sleep complaints, but side effects are a concern in older people because they include an increased risk of falls, fractures and cognitive impairment. Somnolence can be prolonged in older adults. Melatonin has very modest effects on sleep initiation in older adults, although appears safe. Overall, the effects of pharmacological treatments are modest and there are concerns about the efficacy and safety of their ongoing use. There is strong evidence for non‐pharmacological interventions, including cognitive and behavioural strategies, to improve sleep. These have at least comparable efficacy to pharmacological interventions, fewer adverse effects and are more likely to have longer‐term, sustained benefits. Sleep complaints in older adults need to be approached on a patient‐by‐patient basis, and medication should be reviewed frequently, with preference to limit pharmacological intervention as far as practicable.
Effects of dietary isomaltooligosaccharide and Bacillus spp. supplementation during perinatal period on lactational performance, blood metabolites, and milk composition of sows
Authors: Gu Xue L , Song Ze H , Li Hao , Wu Sarah , Wu Shu S , Ding Ya N , He Xi , Yin Yu L , Fan Zhi Y 
Published: May 2019

AbstractBackgroundIn this study, we evaluated the effects of isomaltooligosaccharide (IMO) and Bacillus spp. in perinatal diets on lactational performance, blood metabolites, and milk composition of sows. Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group).ResultsThe average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups. In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05). Furthermore, the highest proportion of fat in colostrum (0.05 < P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05). The concentrations of growth hormone and immunoglobulins A and G in milk were higher in sows supplemented with IMO, IMO + S, IMO + L, or IMO + S + L (P < 0.05).ConclusionIMO and Bacillus spp. supplementation during the perinatal period improved the sows' lactational performance by affecting their metabolism and milk quality. Sows in the IMO + S and IMO + S + L treatment groups exhibited the best performance. © 2019 Society of Chemical Industry
The Israeli–Palestinian wheat landraces collection: restoration and characterization of lost genetic diversity
Authors: Frankin Sivan , Kunta Srinivas , Abbo Shahal , Sela Hanan , Goldberg Ben Z , Bonfil David J , Levy Avraham A , Avivi‐Ragolsky Naomi , Nashef Kamal , Roychowdhury Rajib , Faraj Tomer , Lifshitz Dikla , Mayzlish‐Gati Einav , Ben‐David Roi 
Published: May 2019

AbstractBACKGROUNDFor over a century, genetic diversity of wheat worldwide was eroded by continual selection for high yields and industrial demands. Wheat landraces cultivated in Israel and Palestine demonstrate high genetic diversity and a potentially wide repertoire of adaptive alleles. While most Israeli‐Palestinian wheat landraces were lost in the transition to ‘Green Revolution’ semi‐dwarf varieties, some germplasm collections made at the beginning of the 20th century survived in gene banks and private collections worldwide. However, fragmentation and poor conservation place this unique genetic resource at a high risk of genetic erosion. Herein, we describe a long‐term initiative to restore, conserve, and characterize a collection of Israeli and Palestinian wheat landraces (IPLR).RESULTSWe report on (i) the IPLR construction (n = 932), (ii) the historical and agronomic context to this collection, (iii) the characterization and assessment of the IPLR's genetic diversity, and (iv) a data comparison from two distinct subcollections within IPLR: a collection made by N. Vavilov in 1926 (IPLR‐VIR) and a later one (1979–1981) made by Y. Mattatia (IPLR‐M). Though conducted in the same eco‐geographic space, these two collections were subjected to considerably different conservation pathways. IPLR‐M, which underwent only one propagation cycle, demonstrated marked genetic and phenotypic variability (within and between accessions) in comparison with IPLR‐VIR, which had been regularly regenerated over ∼90 years.CONCLUSIONWe postulate that long‐termex situconservation involving human and genotype × environment selection may significantly reduce accession heterogeneity and allelic diversity. Results are further discussed in a broader context of pre‐breeding and conservation. © 2019 Society of Chemical Industry
Separation of five diketopiperazines from the marine fungus Alternaria alternate HK‐25 by high‐speed counter‐current chromatography
Authors: He Xiaoping , Ding Lijian , Yi Mengqi , Xu Jianzhou , Zhou Xuezhen , Zhang Weiyan , He Shan 
Published: May 2019

AbstractHigh‐speed counter‐current chromatography was applied to the separation of five diketoperazines from the marine Alternaria alternate HK‐25 for the first time using one‐step elution method with a pair of two‐phase solvent systems composed of petroleum ether/ethyl acetate/methanol/water (5.5:11:5:7, v/v). Where 151.6 mg of crude sample yielded five diketoperazines, 12,13‐dihydroxy‐fumitremorgin C (1), gliotoxin (2), demethoxyfum itremorgin C (3), bisdethiobis(methylthio)gliotoxin (4), fumitremorgin C (5), and the purities of all compounds were above 94% as determined by high‐performance liquid chromatography. The structures of these compounds were identified by 1H and 13C NMR spectroscopy. These results showed that high‐speed counter‐current chromatography can provide a feasible way for highly effective preparation of marine natural products, which ensured the supple of numerous samples for drug development.
The last garum of Pompeii: Archaeozoological analyses on fish remains from the “garum shop” and related ecological inferences
Authors: Carannante Alfredo 
Published: May 2019

AbstractThis study represents the first detailed archaeozoological analysis on garum remains from Pompeii. It aims to contribute to our knowledge of activities connected to garum in one of the most celebrated centres of production in the Mediterranean by osteoarchaeological, taphonomical, and ecological analyses. One place for the production and sale of this fish sauce was located in the urban perimeter of the town, the so‐called “garum shop”, buried by the pyroclastic deposits of the Vesuvius eruption in 79 AD. After a short summary about the nature, role and origin of garum, and its production processes, a description of the state of the art about garum production in Pompeii is summarized to assess the issue. The analyses undertaken on the remains found in an amphora from the “garum shop” show that the production of at least one kind of garum in Pompeii was obtained using individuals of picarels (Spicara smaris) as the fundamental ingredient. The state of preservation of the fish remains indicates that the picarels were not subject to any intense process of movement or compression that could have caused breakage at fragile points of the fish skeletons; it is therefore deduced that the product was still in the maceration stage awaiting the final process when the Pompeii destruction happened. The study revealed that the picarels were left to macerate whole; they were neither decapitated nor filleted. The osteoarchaeological and ecological analyses revealed the age of death, the sex, and the season when the fish was caught contributing to define the fishing techniques, the production processes, and the period of the year when the volcanic destruction happened.
Positive psychological experiences in allogeneic hematopoietic stem cell transplantation
Authors: Amonoo Hermioni L. , Brown Lydia A. , Scheu Carlyn F. , Millstein Rachel A. , Pirl William F. , Vitagliano Halyna L. , Antin Joseph H. , Huffman Jeff C. 
Published: May 2019

AbstractObjectivePositive psychological constructs (e.g., optimism and hope) have been associated with superior health outcomes in the recovery from hematopoietic stem cell transplantation (HSCT) in prior work. However, such studies have not examined a broad range of positive psychological constructs, their sources or evolution over time, or their connections with key health behaviors. We used semistructured qualitative interviews in 25 HSCT patients to explore the nature and sources of positive psychological experiences during the first 100 days after transplantation.MethodsParticipants completed interviews during their HSCT hospitalization and approximately 100 days after transplantation. Transcribed interviews were organized and analyzed using NVivo 12 software by two coders (κ = 0.92). Interviews focused on the breadth of positive psychological experiences, their sources and evolution during the 100 days, and participants' perceived impact of positive constructs on health behaviors.ResultsSeveral positive psychological constructs especially gratitude, determination, and optimism were frequently expressed. Family support and deliberate participation in pleasant and meaningful activities were the two primary sources of positive psychological experiences after HSCT. At baseline, participants consistently reported gratitude for their donors while follow‐up was consistently characterized by hope for cure and return to baseline level of functioning. Additionally, participants related bidirectional relationships between positive psychological well‐being experiences and completion of health behaviors over time.ConclusionsAs one of the first qualitative studies characterizing the breadth, sources, and evolution of positive psychological experiences in HSCT patients, these findings add to the literature aimed at understanding psychological well‐being in this complex and growing patient population.
The effects of slow loaded breathing training on exercise blood pressure in isolated systolic hypertension
Authors: Ubolsakka‐Jones Chulee , Tongdee Phailin , Jones David A. 
Published: May 2019

AbstractObjectivesSlow loaded breathing training has been shown to reduce resting blood pressure (BP) in isolated systolic hypertension (ISH), but it is not known whether this also reduces their exaggerated BP responses to exercise.MethodsThe study was a randomized controlled trial with block allocation stratified by sex. Twenty ISH patients (68 ± 5 yrs, 11 males) were randomized with one group undertaking 8‐weeks training with slow loaded breathing (SLB: 25% maximum inspiratory pressure, 6 breaths per minute, 60 breaths every day) or deep breathing control (CON), with 8 weeks follow‐up. Outcome measures were home BP and heart rate (HR) with laboratory measures of BP and HR responses to static handgrip and dynamic arm cranking exercise. Data were compared with a two‐week run‐in baseline.ResultsHome systolic BP fell by 22 mmHg (20–23; mean, 95% CI), diastolic BP by 9 mmHg (7–11), and HR by 12 bpm (9–15; all p < .001) as a result of SLB training. Systolic BP at the end of 2‐min isometric handgrip was 189 ± 10 mmHg (mean, SD) before training and 157 ± 6 mmHg following SLB training. After 4‐min arm exercise, systolic BP, measured at the ankle, was reduced from 243 ± 8 mmHg during the run‐in period to 170 ± 15 mmHg after SLB training with no change for CON. The reduction in exercise BP, in both types of exercise, was partly due to a reduction in resting BP and to a smaller increase above resting. Systolic and pulse pressures remained below run‐in values 8 weeks after the end of SLB training, and BP response to handgrip exercise remained below run‐in values at 4 weeks after SLB training.ConclusionsSLB not only reduces resting BP in ISH but also the responses to both static and dynamic exercise, potentially reducing the negative aspect of exercise for cardiovascular health.