bluuebunny/crossref_metadata_2025_split huggingface dataset:
Excessive treadmill training enhances the insulin signaling pathway and glycogen deposition in mice hearts
Authors: Oliveira Luciana da C. , de Morais Gustavo P. , da Rocha Alisson L. , Teixeira Giovana R. , Pinto Ana P. , de Vicente Larissa G. , Pauli José R. , de Moura Leandro P. , Mekary Rania A. , Ropelle Eduardo R. , Cintra Dennys E. , da Silva Adelino S. R. 
Published: October 2018

AbstractExhaustive and chronic physical exercise leads to peripheral inflammation, which is one of the molecular mechanisms responsible for the impairment of the insulin signaling pathway in the heart. Recently, 3 different running overtraining models performed downhill (OTR/down), uphill (OTR/up), and without inclination (OTR) increased the serum levels of proinflammatory cytokines. This proinflammatory status induced insulin signaling impairment in the skeletal muscle; however, the response of this signaling pathway in the cardiac muscle of overtrained mice was still unknown. Thus, we investigated the effects of OTR/down, OTR/up, and OTR protocols on the protein levels of phosphorylation of insulin receptor β (pIRβ) (Tyr), phosphorylation of protein kinase B (pAkt) (Ser473), plasma membrane glucose transporter‐1 (GLUT1) and GLUT4, phosphorylation of insulin receptor substrate‐1 (pIRS‐1) (Ser307), phosphorylation of IκB kinase α/β) (pIKKα/β (Ser180/181), phosphorylation of p38 mitogen‐activated protein kinase (p‐p38MAPK) (Thr180/Tyr182), phosphorylation of stress‐activated protein kinases‐Jun amino‐terminal kinases (pSAPK‐JNK) (Thr183/Tyr185), and glycogen content in mice hearts. The rodents were divided into naïve (N, sedentary mice), control (CT, sedentary mice submitted to performance evaluations), trained (TR, performed the training protocol), OTR/down, OTR/up, and OTR groups. After the grip force test, the cardiac muscles (ie, left ventricle) were removed and used for immunoblotting and histology. Although the OTR/up and OTR groups exhibited higher cardiac levels of pIRβ (Tyr), only the OTR group exhibited higher cardiac levels of pAkt (Ser473) and plasma membrane GLUT4. On the contrary, the OTR/down group exhibited higher cardiac levels of pIRS‐1 (Ser307). The OTR model enhanced the cardiac insulin signaling pathway. All overtraining models increased the left ventricle glycogen content, with this probably acting as a compensatory organ in response to skeletal muscle insulin signaling impairment.
DISC1 gene polymorphisms and the risk of schizophrenia in an Iranian population: A preliminary study
Authors: Shokouhifar Alireza , Askari Nasrin , Yazdani Shaghayegh , Mehrabadi Jalil Fallah 
Published: October 2018

AbstractBackground: Schizophrenia, schizoaffective disorder, and bipolar illness are common psychological disorders with high heritability and variable phenotypes. The disrupted in schizophrenia 1 (
DISC1) gene, on chromosome 1q42, has an essential role in neurite outgrowth and cell signaling. The purpose of this study was to investigate the association of three single‐nucleotide polymorphisms (SNPs; rs6675281, rs2255340, and rs2738864) with schizophrenia disorder. These three SNPs were chosen as they had been used in most of the previous studies.Methods: In a case‐control study of Iranian population for the first time 778 blood samples were collected including, 402 schizophrenic patients and 376 healthy controls. Genomic DNA was extracted from peripheral blood using DNA extraction kit (BioFlux Co). The genotypes of rs6675281, rs2255340, and rs2738864 were detected by nested allele‐specific multiplex polymersae chain reaction (PCR).Results: Our data revealed that the three SNPs are significantly associated with schizophrenia (rs2255349 C>T: confidence interval (CI), 2.115 to 3.268; P = 0.0000 OR: 2.629; rs2738864 C>T: CI, 1.538 to 2.339; P = 0.0000 OR: 1.897; rs6675281 C>T: CI, 2.788 to 4.662; P = 0.0009241 OR: 3.605). Through applying the expectation‐maximization (EM) algorithm, we calculated the haplotype frequency, and finally performed haplotype analysis with Bonferroni correction and data preprocessing methods and the results showed rs66875281 to have the highest association.Discussion: Our findings primarily showed that DISC1 gene polymorphisms contribute to schizophrenia risk and have a significant association with this disorder among Iranian population. The strategy was found to be easy, rapid, specific, and consistent for the co‐occurring detection of the DISC1 polymorphisms. We could finally confirm that the polymorphisms are related to schizophrenia studied in Iranian population.
4‐O‐carboxymethylascochlorin protected against microglial‐mediated neurotoxicity in SH‐SY5Y and BV2 cocultured cells from LPS–induced neuroinflammation and death by inhibiting MAPK, NF‐κB, and Akt pathways
Authors: Park Junyoung , Ha Sun‐Hyung , Abekura Fukushi , Lim Hakseong , Chang Young‐Chae , Lee Moon‐Jo , Lee Miri , Lee Young‐Choon , Kim Cheorl‐Ho 
Published: October 2018

AbstractIn our previous studies, structurally similar compounds of ascochlorin and ascofuranone exhibited anti‐inflammatory activity. Neural inflammation plays a significant role in the commence and advancement of neurodegenerative diseases. It is not known whether 4‐O‐carboxymethylascochlorin (AS‐6) regulates the initial stage of inflammatory responses at the cellular level in BV2 microglia cells. We here investigated the anti‐inflammatory effects of AS‐6 treatment in microglia cells with the microglial protection in neurons. We found that the lipopolysaccharide (LPS)‐stimulated production of nitric oxide, a main regulator of inflammation, is suppressed by AS‐6 in BV2 microglial cells. In addition, AS‐6 dose‐dependently suppressed the increase in COX‐2 protein and messenger RNA levels in LPS‐stimulated BV2 cells. Moreover, AS‐6 inhibited the expression and secretion of proinflammatory cytokines in BV2 microglial cells. At the intracellular level, AS‐6 inhibited LPS‐activated nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB) in BV2 microglial cells. AS‐6 negatively affected mitogen‐activated protein kinases (MAPK) and Akt phosphorylation: Phosphorylated forms of ERK, JNK, p38, and Akt decreased. To check whether AS‐6 protects against inflammatory inducer‐mediated neurotoxicity, neuronal SH‐SY5Y cells were coincubated with BV2 cells in conditioned medium. AS‐6 exerted a neuroprotective effect by suppressing microglial activation by LPS or amyloid‐β peptide. AS‐6 is a promising suppressor of inflammatory responses in LPS‐induced BV2 cells by attenuating NF‐κB and MAPKs signaling. AS‐6 protected against microglial‐mediated neurotoxicity in SH‐SY5Y and BV2 cocultured cells from LPS–induced neuroinflammation and death via inhibiting MAPK, NF‐κB, and Akt pathways.
Differential expression profiles of long noncoding RNAs in synchronous multiple and solitary primary esophageal squamous cell carcinomas: A microarray analysis
Authors: Li Qi‐Wen , Ma Li , Qiu Bo , Yang Hong , Zhu Yu‐Jia , Qiang Meng‐Yun , Liu Song‐Ran , Chen Nai‐Bin , Guo Jin‐Yu , Cai Ling‐Zhi , Wang Jun‐Ye , Zhang Xu , Liu Hui 
Published: October 2018

AbstractAs a unique subtype of esophageal cancer, synchronous multiple primary esophageal squamous cell carcinomas (ESCCs) mostly occur in Asian patients with alcohol and/or tobacco abuse, or with a family history of cancer. Multiple ESCCs are associated with poor clinical outcomes. Growing evidence has addressed that long noncoding RNAs (lncRNAs) are involved in the carcinogenesis of various malignancies. We compared the lncRNA and messenger RNA (mRNA) profiles between solitary and multiple ESCC tissues through microarray analysis, aiming at studying their different mechanisms in tumor development. As a result, in multiple ESCCs, a total of 5257 lncRNAs and 3371 mRNAs were consistently differentially expressed compared with solitary ESCC, including 2986 upregulated and 2271 downregulated lncRNAs, and 2313 upregulated, and 1058 downregulated mRNAs. We validated the results in four differentially expressed lncRNAs using quantitative real‐time polymerase chain reaction. There were 38 and 20 pathways significantly related to up‐ and downregulated transcripts. The pathways associated with mostly enriched up‐ and downregulated mRNAs were hsa01200 (carbon metabolism) and hsa05221 (acute myeloid leukemia‐
homo sapiens [human]). Gene ontology analysis suggested that upregulated and downregulated mRNAs were mainly enriched in bounding membrane of organelle involved in the cellular component and positive regulation of transport involved in the biological process. Further analysis identified 189 differentially expressed paired antisense lncRNAs and relative sense mRNA, as well as 2134 differentially expressed long intergenic noncoding RNAs and their adjacent mRNA pairs. In conclusion, the aberrantly expressed lncRNAs might play a role in the carcinogenesis of multiple ESCCs and could be candidates as diagnostic biomarkers and therapeutic targets.
Long noncoding RNA NNT‐AS1 promotes gastric cancer proliferation and invasion by regulating microRNA‐363 expression
Authors: Wang Xin , Ren Mudan , Li Yarui , Hu Junbi , Lu Guifang , Ma Wenhui , Guo Dan , Lu Xinlan , He Shuixiang 
Published: October 2018

AbstractIncreasing studies showed that long noncoding RNAs (lncRNAs) had crucial regulatory roles in various tumors, including gastric cancer (GC). Recent studies demonstrated that lncRNA nicotinamide nucleotide transhydrogenase‐antisense RNA1 (NNT‐AS1) played an important role in several tumors. However, the role and expression of NNT‐AS1 in GC progression remain unknown. In our study, we indicated that NNT‐AS1 expression was upregulated in GC samples compared with the nontumor tissues. We also showed that NNT‐AS1 expression was upregulated in the GC cell lines. Ectopic expression of NNT‐AS1 promoted GC cell line HGC‐27 cell proliferation, cell cycle progression, and invasion. In addition, we showed that NNT‐AS1 acted as a sponge competing endogenous RNA for microRNA‐363 (miR‐363), which was downregulated in the GC samples and cell lines. miR‐363 expression was negatively related with NNT‐AS1 expression in GC samples. Upregulated expression of miR‐363 suppressed GC cell growth, cycle, and invasion. Furthermore, we reported that elevated expression of NNT‐AS1 promoted GC cell proliferation, cycle, and invasion partly by suppressing miR‐363 expression. These results indicated that lncRNA NNT‐AS1 acted as an oncogene in the development of GC partly by inhibiting miR‐363 expression.
Vancomycin elution kinetics from porous tantalum metal
Authors: Sautet Pierre , Mékidèche Thibaut , Guilhaumou Romain , Abdel Matthew P. , Argenson Jean‐Noël , Parratte Sébastien , Ollivier Matthieu 
Published: October 2018

ABSTRACTRevisions TKAs are being completed with uncemented constructs more frequently. We hypothesized that tantalum cones could be an efficient carrier of antibiotics in uncemented procedures. We aimed to compare the release of vancomycin between (i) tantalum and smooth stainless cylinders; (ii) different concentrations of vancomycin; and (iii) different durations of bathing. Specifically designed tantalum cylinders were bathed in a vancomycin solution with various durations of baths. We investigated rinses between each interval as well as the dose of vancomycin. Vancomycin concentrations were determined in each group by fluorescence polarization immunoassay at different intervals (1 h, days 1, 2, 3, 5). At 1 h, the mean vancomycin concentration for the 1‐hour soaking group was 3,172 μg/ml, whereas mean concentration for the smooth stainless steel group was 39.37 μg/ml (p < 0.001). The rinsing group showed a significantly lower concentration at 1 h and 1 day (p < 0.05). The 2‐gram vancomycin group showed no difference at days 1, 2, and 3 compared to the 1‐hour group. The 5, 15, and 30‐minute bathing groups showed significantly lower vancomycin concentrations at all‐time points. All vancomycin concentrations at day 3 were superior to the minimal inhibitory concentration of Staphyloccocus aureus. The mean concentration of vancomycin depends on the material, duration of bathing, the rinsing effect, and the drug dose. Our in‐vitro study is the first to show that porous tantalum cylinders allow antibiotic carriage and progressive release. If appearing in vivo, in a similar extent, this intrinsic property might be useful to prevent and/or treat peri‐prosthetic joint infection. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:308–312, 2019.
Studies on Biosurfactants Produced using Bacillus cereus Isolated from Seawater with Biotechnological Potential for Marine Oil‐Spill Bioremediation
Authors: Durval Italo José B. , Resende Ana Helena M. , Figueiredo Mariana A. , Luna Juliana M. , Rufino Raquel D. , Sarubbo Leonie A. 
Published: October 2018

AbstractWith the aim of producing a biotensioactive material for use in the remediation of marine environments, screening for biosurfactant‐producing bacteria was conducted with strains isolated from seawater contaminated with petroleum derivatives. Gene sequencing revealed that all four promising biosurfactant‐producing isolates belonged to the same genus and species, namely Bacillus cereus. The biosurfactant‐producing bacteria were cultivated with different carbon (glucose, soybean oil, and waste frying soybean oil) and nitrogen (ammonium chloride, sodium nitrate, urea, and peptone) sources. B. cereus strain BCS0 was chosen as the best biosurfactant producer in a mineral medium with 2% frying oil and 0.12% peptone. Following the optimization of agitation and cultivation time, an agitation rate of 250 rpm and 48 h of cultivation were selected. Under these conditions, the surface tension was reduced to 27 mN m−1 and the biosurfactant concentration was 3.5 g L−1. The critical micelle concentration (CMC) of the biosurfactant was defined as 500 mg L−1. The biosurfactant remained stable within large ranges of pH (2–10), salinity (2–10%), and temperature (5–120 °C). Under these conditions, motor oil emulsification rates were greater than 90%. Moreover, the biosurfactant properties remained unaltered after heating at 90 °C for 120 min. The biosurfactant enhanced the degradation of motor oil up to 96% in 27 days and exhibited considerable motor oil displacement capacity. Thus, the biosurfactant has potential in the application of remediation processes in marine environments.
Protective effects of antioxidant egg‐chalaza hydrolysates against chronic alcohol consumption‐induced liver steatosis in mice
Authors: Yang Kuo‐Tai , Lin Yi‐Ling , Lin Yu‐Xuan , Wang Sheng‐Yao , Wu Yi‐Hsieng S , Chou Chung‐Hsi , Fu Shih‐Guei , Chen Yi‐Chen 
Published: October 2018

AbstractBACKGROUNDReactive oxygen species (ROS) overproduction is highly related to some human chronic diseases. There are approximately 400 metric tons of chalazae produced yearly after the processing of the liquid‐egg production, which are disposed of as waste. The objectives of this study were to look for the optimal production condition of antioxidant crude chalaza hydrolysates and evaluate the in vivo antioxidant capacity via a chronic alcohol consumption mouse model.RESULTSAntioxidant crude chalaza hydrolysates (CCH‐As) could be produced by protease A at 1:100 ratio (w/w) and 0.5 h hydrolytic period. After our analyses, CCH‐As were rich in leucine, arginine, phenylalanine, valine, lysine and antioxidant dipeptides (anserine and carnosine), and the major molecular masses were lower than 15 kDa. Regarding protective effects of CCH‐As against oxidative damage in alcoholic‐liquid‐diet‐fed mice, alcohol‐fed mice had lower (P < 0.05) liver antioxidant capacities, and higher (P < 0.05) liver lipid contents, serum lipid/liver damage indices and IL‐1β/IL‐6 values. CCH‐A supplementation reversed (P < 0.05) liver antioxidant capacities and reduced (P < 0.05) serum/liver lipids in alcohol‐fed mice, which may result from increased (P < 0.05) fecal lipid output, upregulated (P < 0.05) fatty acid β‐oxidation and downregulated (P < 0.05) lipogenesis in the liver.CONCLUSIONTaken together, this CCH‐A should benefit the liquid‐egg industry, while also offering consumers a choice of healthy ingredients from animal sources. © 2018 Society of Chemical Industry
Application of apigeninidin‐rich red sorghum biocolorant in a fermented food improves product quality
Authors: Akogou Folachodé UG , Canoy Tessa S , Kayodé Adéchola PP , den Besten Heidy MW , Linnemann Anita R , Fogliano Vincenzo 
Published: October 2018

AbstractBACKGROUNDThe ‘clean label’ trend is pushing the food industry to replace synthetic colorants with plant‐based colorants. However, technological efficacy and undesirable side effects restrict the use of plant‐based colorants in industrial applications. This research studied the production of fermented maize dough coloured by apigeninidin‐rich red sorghum biocolorant, as practised for centuries in West Africa, as a model to assess the impact of the biocolorant on nutritional and sensorial quality of foods.RESULTSA 3‐day fermentation of a dyed maize dough (containing 327 µg g−1dry matter of apigeninidin) byPichia kudriavzeviiandLactobacillus fermentumled to a degradation of 69% of the apigeninidin content, causing a clearly visible colour difference (ΔE*0017.4). The antioxidant activity of fermented dyed dough (DD) increased by 51% compared to fermented non‐dyed dough (NDD). However, the phytate dephosphorylation and volatile organic compound concentrations were lower in DD than in NDD. This suggests a lower mineral solubility and change in the sensory quality of fermented DD.CONCLUSIONApigeninidin extract from sorghum leaf sheaths proved to be a bioactive red biocolorant with potential in fermented foods. The formation of new antioxidant compounds needs further investigation, as does the impact on the development of volatile compounds. © 2018 The Authors.Journal of The Science of Food and Agriculturepublished by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Effects of thermal treatment on walnut detection and allergenicity
Authors: Vencia Walter , Minale Paola , Migone Laura , Lazzara Fabrizio , Vito Guendalina , Ferrari Angelo , Razzuoli Elisabetta 
Published: October 2018

AbstractBACKGROUNDPeanuts and tree nut allergies pose an increasing food safety problem. The aim of our study was to test the accuracy of different commercial enzyme‐linked immunosorbent assay (ELISA) kits in the detection of the presence of walnuts in untreated and heat exposed food samples. The effects of thermal treatment of samples were evaluated by exposing walnuts to different heat treatments. All samples were first analysed by two different commercial ELISA assays. Then, we performed a skin prick test (SPT) on nine patients with proven nut allergy using small walnut pieces from raw and treated samples.RESULTSThe presence of nuts proteins in thermally processed foods was not accurately detected by ELISA kits. All patients had a positive SPT reaction with raw walnut, while thermal treatments affected walnut allergenicity. The ELISA test gives a negative result in the case of strong thermal treatment, but at the same time allergic subjects react positively to stimulation with the same sample.CONCLUSIONThis study suggests that commercial ELISA kits may not be able to accurately determine the amount of proteins present in thermally processed foods due to changes in the solubility and immunoreactivity of the target proteins. Finally, the clinical results highlight that thermal treatment might induce a reduction in walnut allergenicity. © 2018 Society of Chemical Industry
Antibacterial and antioxidative properties of different parts of garden rhubarb, blackcurrant, chokeberry and blue honeysuckle
Authors: Raudsepp Piret , Koskar Julia , Anton Dea , Meremäe Kadrin , Kapp Karmen , Laurson Peeter , Bleive Uko , Kaldmäe Hedi , Roasto Mati , Püssa Tõnu 
Published: October 2018

AbstractBACKGROUNDIt is important to find plant materials that can inhibit the growth of Listeria monocytogenes and other food‐spoiling bacteria both in vitro and in situ. The aim of the study was to compare antibacterial and antioxidative activity of selected plant–ethanol infusions: leaves and berries of blackcurrant (Ribes nigrum L.), berries of chokeberry (Aronia melanocarpa (Michx.) Elliott) and blue honeysuckle (Lonicera caerulea L. var. edulis); petioles and dark and light roots of garden rhubarb (Rheum rhaponticum L.) for potential use in food matrices as antibacterial and antioxidative additives.RESULTSThe strongest bacterial growth inhibition was observed in 96% ethanol infusions of the dark roots of rhubarbs. In 96% ethanol, nine out of ten studied plant infusions had antibacterial effect against L. monocytogenes, but in 20% ethanol only the infusions of dark rhubarb roots had a similar effect. Chokeberry and other berries had the highest antioxidative activity, both in 20% and 96% ethanol infusions.CONCLUSIONThe combination of dark rhubarb roots or petioles and berries of black chokeberry, blackcurrant or some other anthocyanin‐rich berries would have potential as both antibacterial and antioxidative additives in food. © 2018 Society of Chemical Industry
Depth of invasion in superficial oral tongue carcinoma quantified using intraoral ultrasonography
Authors: Iida Yoshiyuki , Kamijo Tomoyuki , Kusafuka Kimihide , Omae Katsuhiro , Nishiya Yukio , Hamaguchi Noriko , Morita Kotaro , Onitsuka Tetsuro 
Published: October 2018

ObjectiveDepth of invasion (DOI) in oral carcinoma has been integrated into the primary tumor categories in the current tumor‐node‐metastasis staging (8th edition). However, there is no standard modality to determine DOI preoperatively. The aims of the present study were to evaluate the accuracy of a preoperative measurement of DOI using ultrasonography (US) for superficial oral tongue carcinomas, and to correlate the values obtained with histologically determined DOI measurements.MethodsWe retrospectively analyzed the records of 56 patients with oral tongue carcinoma who underwent intraoral US preoperatively, followed by curative surgery at the Shizuoka Cancer Center Hospital in Japan. For the measurement of DOI with US, our unique technique (water balloon method) was evaluated.ResultsThe histologically measured tumor size (maximum diameter) showed a distribution of 7.0 to 40.0 mm (mean, 18.6 mm). The correlation between the US‐obtained and histologically obtained DOIs was significant (r = 0.867; P < 0.001). In tumors characterized histologically as superficial (DOI ≤ 5 mm), a significant correlation was observed between US‐obtained and histologically obtained DOIs (r = 0.870, P  < 0.001). For the entire cohort, the sensitivity and specificity of US assessment of DOI of ≤ 5 mm or > 5 mm were 92.3% and 70.6%, respectively.ConclusionIntraoral US provides sufficient accuracy for the measurement of tumor DOI in oral tongue carcinoma and is complementary in assessing superficial lesions.Level of Evidence4. Laryngoscope, 128:2778–2782, 2018