bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of Selected Antibiotics on the Tomato Regeneration in In Vitro Cultures
Authors: GERSZBERG Aneta , GRZEGORCZYK-KAROLAK Izabela 
Published: July 2019

Generally, antimicrobial agents are frequently used in micropropagation techniques to obtain free elite clones or after genetic transformation to select putative transformants. Their successful application minimizes bacterial contamination however; they may be phytotoxic and may diversely affect the regeneration ability in plant tissue cultures. The objective of the current study was to estimate the effects of four antibiotics i.e. ampicillin, carbenicillin, cefotaxime and kanamycin on morphogenesis of three Polish tomato cultivars, cultured on MS medium with phytohormones. In this experiment the ability of tomato cotyledon explants to regenerate entire plants via indirect organogenesis was tested. Among four antibiotics tested, kanamycin was most harmful for the explants. This antibiotic, even at low doses (10-20 mg/L), inhibited tomato morphogenesis. On the other hand, the current study revealed significant influence of different concentrations of ampicillin, carbenicillin, cefotaxime on the frequency of bud formation. While the addition of cefotaxime at low concentration (100-200 mg/L) stimulated the bud formation, its increasing concentration adversely affected the organogenesis of tomato. The results clearly pointed out that carbenicillin and ampicillin at low concentrations (100-400 mg/L) were not only non-toxic, but they promoted bud regeneration. The obtained results show the crucial role not only of use of efficient antibiotics, but also of their proper doses in obtaining successful transformation and regeneration of tomato.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Biocontrol of Pear Postharvest Decay by Kombucha
Authors: TAN Junping , ZHOU Xian , CHENG Shuiyuan , CHEN Zexiong , GOU Yuanyuan , YE Jiabao , XU Feng 
Published: July 2019

Kombucha is sweetened, slightly alcoholic, and lightly effervescent tea drink. Its acidity inhibits the growth of harmful bacteria. Here, we studied effect of kombucha on post-harvest preservation of pear. The Hosui pears were soaked with the kombucha for 15 minutes, and in distilled water as the control, respectively. Superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), hydrogen peroxide (H2O2), proline content, electric conductivity, weight loss rate and good fruit rate in pear were measured during storage at room temperature. The results showed that the kombucha could effectively prolong the storage time of pear fruit. The fruit quality of the treated group was 1.5 times higher than that of the control pear fruit when stored at room temperature for 18 days. The weight loss rate, MDA content and electric conductivity of the treatment group were lower than those of the control group during the whole storage period, indicating that kombucha can inhibit the transformation of polysaccharides such as starch and pectin, and delay the degradation of nutrients in the fruit, resulting in a decrease in weight loss rate, inhibition of membrane lipid peroxidation, reducing MDA content, electric conductivity, maintaining cell membrane stability, delaying pear fruit senescence. Kombucha can inhibit the content of H2O2 and proline, increase the activity of POD and SOD increase the storage stability of fruits. The purpose of this study was to elucidate the physiological mechanism of post-harvest preservation of pear by kombucha. The use of kombucha can prolong the supply period of pear, increase economic benefits and expand the market of pear.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Optimization of the Solid-Liquid Extraction Process of Phenolic Compounds from Mulberry Fruit
Authors: KOSTIĆ Emilija , ARSIĆ Biljana , MITIĆ Milan , DIMITRIJEVIĆ Danica , PECEV MARINKOVIC Emilija 
Published: July 2019

The study was designed to examine the influence of solvent concentration (ethanol/water 20-80% by volume), extraction time (15-240 min) and extraction technique on the extraction yield of phenolic compounds, flavonoids and antioxidant activity from Morus nigra L., Morus rubra L. and Morus alba L. fruits. The optimum conditions for the extraction of total phenols, flavonoids and monomeric anthocyanins by the maceration and ultrasonic extraction processes were: 213.6 min with 80% ethanol, and 182.1 min and 71.2% ethanol, respectively (black mulberry), and 216.5 min with 78.3% ethanol and 198.7 min and 70.6% ethanol, respectively (red mulberry). In case of white mulberry for the extraction of total phenols and flavonoids optimum extraction conditions (maceration and ultrasonic) were 232.7 min and 80% ethanol, and 187.2 min and 68.7% ethanol, respectively (white mulberry). Obviously, ultrasonic extraction was less time consuming, and it requires for all performed extractions solvent with less percentage of ethanol.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Transcriptome-based Discovery of AP2/ERF Transcription Factors Related to Terpene Trilactones Synthesis in Ginkgo biloba
Authors: LI Linling , YUAN Honghui , ZHA Sanxing , YU Jie , XIAO Xian , DENG Kun , CHENG Shuiyuan , CHENG Hua 
Published: July 2019

Ginkgo biloba is a unique tree in China with medicinally and phylogenetically important characteristics. Terpene trilactones (TTL) is a key active pharmaceutical ingredient in Ginkgo, so the content of TTL in Ginkgo has become one of the important indices for evaluating quality of the medicinal materials. By transcriptome sequencing on samples treated by chlormequat, ultraviolet (UV) and drought, totally 59820 contigs and 37564 unigenes were obtained. Furthermore, 18234 unigenes were annotated through COG, KEGG and GO analysis. There were 78 AP2/ERF transcription factors, 23 factors of up-regulation and 66 factors of down-regulation that were related with synthetic pathway of TTL in Ginkgo. Phylogenetic tree clustering analysis indicated that there were 42 AP2s could be clustered into ERF, DREB and RVA subfamilies. EMSA analysis demonstrated that GbERF13, GbERF25 and GbERF27 could bind with regulatory elements, such as E-box, in the upstream of GbMECPs promoter. Expression analysis showed that the expression level of GbERF25 was the highest in root, and GbERF25 and GbERF27 were expressed in relatively high transcription levels in leaf and other tissues. The results of qRT-PCR indicated that CCC treatment could significantly improve expression levels of ERF25 and ERF27, and UV and drought could induce transcription levels of ERF13 and ERF25, respectively. The results implied that ERF25 and ERF27 might involve in the induction and regulation of CCC treatment on synthesis of bilobalide in G. biloba. ERF13 might participate in the regulation of bilobalide synthesis induced by UV, and EFR25 might involve in the regulation of the synthesis induced by drought. During annual cycle of expression, the transcription levels of ERF13, ERF25 and ERF27 had significantly positive correlation with diterpene level with correlation coefficient 0.975. It implied that these transcription factors mainly acted on the MEP pathway that regulated synthesis of bilobalide. The aim of the research was to indicate the mechanism of environment or cultivation measure regulating target gene of TTL metabolic pathway by AP2/ERF, and establish metabolic network of AP2/ERF regulating TTL synthesis.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Cytological Investigation in Two Mutants of the Hybrid Grapevine ‘Niagara’
Authors: PIEROZZI Neiva Izabel , MOURA Mara Fernandes 
Published: July 2019

‘Niagara’ is a hybrid table grape (V. vinifera × V. labrusca). Several spontaneous somatic mutants from this grapevine have already been described. However, cytological information about these mutants is scarce. Therefore, studies in the microsporogenesis have been carried out in two of these mutants, a seedless and a large berry mutant (giant mutant). A low percentage of abnormalities such as precocious chromosome segregation, bivalent not aligned, laggard and stickiness chromosomes were seen in both mutants. A merging process of microspores was observed in several tetrads in the seedless mutant: the microspore came together by large cytoplasmic bridges giving rise to a large and amorphous single-celled tetrad, which eventually collapsed. The giant mutant showed 10.29% of tetrads with microcytes. However, these tetrad abnormalities did not hinder pollen viability. Significant differences were seen concerning stomata and pollen grain sizes, stomata frequency and anther number per flower between the mutants. The chromosome count at diakinesis (n=19) and in diploid tapetal cells (2n=38) clarified the fact that the seedless somatic mutation was not associated with any ploidy event. Conversely, the giant mutant showed n=38 at diakinesis and 2n=76 in diploid tapetal cells, pointing to a case of spontaneous chromosome duplication. Since these abnormalities did not disturb the percentage of pollen viability very much, in both mutants (higher than 86%), the seedless one may be used as a male parent in crossing, regarding the desirable seedlessness character, while the mutant giant may be employed as female parent for the obtainment of seedless triploid plants.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Some Biochemical Characteristics of Fruit Samples of Sambucus nigra Genotypes from Northeastern Turkey
Authors: KAN Tuncay 
Published: July 2019

There are numerous wild edible fruit species in the world and they are important components of dietary utilization, local pharmacopoeia, cultural and religious practices of indigenous people. In this study, some important berry characteristics of seven seed propagated black elderberry (Sambucus nigra) genotypes sampled from Rize province, Turkey, were assessed. The genotypes exhibited a great diversity on most of the biochemical characteristics. Berries of genotypes showed moderate Vitamin C content ranged from 26 to 38 mg per 100 g fresh weight. Total phenolic and total anthocyanin content in fruits of the S. nigra genotypes has also high value ranged from 360 to 447 mg gallic acid equivalent (GAE) per 100 g fresh weight and 307 to 412 mg cyanidin-3-glucoside equivalent per 100 g fresh berry, respectively. Antioxidant activity was found between 5.28 and 7.11 mmol trolox equivalent per 100 g fresh weight basis. Citric acid and fructose determined as major organic acid and sugar for all genotypes. For its high vitamin C, total anthocyanin and total phenolic content and high antioxidant activity of fruits of wild grown S. nigra may have provide many health benefits and can be domesticated to establish orchards in Turkey.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Characterization and Tissue-specific Expression of bHLH Genes in Dimocarpus longan
Authors: ZHENG Wei , DONG Xueming , YU Xuefei , ZHANG Qiuying , CHEN Ning 
Published: July 2019

In plants, the basic helix-loop-helix (bHLH) transcription factors (TFs) play pivotal roles in many biological processes including growth, stress response, and secondary metabolite synthesis. To date, many bHLH genes have been identified and characterized in diverse plant species. However, little is known regarding the bHLH genes in Dimocarpus longan Lour. (D. longan). Based on RNA-seq data, we identified 42 putative bHLH genes from D. longan and determined their putative functions using the NCBI Conserved Domain Search Tool and Pfam databases. The physicochemical properties, phylogenetic relationships, conserved motifs, gene ontology (GO) annotations, protein-protein interactions, and tissue-specific expression patterns of these bHLH genes were systematically explored. In total, ten motifs were found in DlbHLH proteins using MEME, among which two were highly conserved. Phylogenetic tree analysis found that DlbHLH proteins can be divided into nine groups, with group 2 being the largest. GO annotation results showed that the DlHLH genes were involved in various molecular functions. RNA-seq and qRT-PCR results revealed important differences in the expression patterns of 17 of the DlbHLH genes. In particular, DlbHLH-9, DlbHLH-19, DlbHLH-25, DlbHLH-26, and DlbHLH-35 were found to show significantly different expression patterns in root and leaf tissues. The results of this study will further enrich our knowledge regarding bHLH transcription factor genes and lay a foundation for enhancing the production of active secondary metabolites by genetic engineering in D. longan.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Various Light Quality including QD-LED Affect Growth and Leaf Color of Red Romaine Baby Leaf Lettuce
Authors: HAN Su Jung , CHOI In-Lee , KIM Ju Young , WANG Lixia , LEE Joo H. , CHOI Ki-Young , KIM Yongduk , ISLAM Mohammad Z. , LEE Young-Tack , KANG Ho-Min 
Published: July 2019

Light quality has a substantial effect on crops in plant factories. Quantum dot (QD) refers to ultrafine semiconductor particles and is expressed in a variety of wavelength ranges and fine and precise colors and is attracting attention as a next-generation material. This study was conducted to investigate the effects of various light qualities, including a QD-LED light source on the growth and color development of red romaine lettuce in the LED chamber system for plant factories. The light source was red fluorescent (FL), blue (B), red (R), blue + red mixed light (BR), and blue + wide range red + red mixed light QD-LED. The growth index, chlorophyll content, and leaf color were examined. The results showed that the plant length was long without a significant difference in QD-LED and the red (R) wavelength and showed the fastest growth under QD-LED. The photosynthetic rate and chlorophyll content were the highest in QD-LED light with a broad spectrum of red light. The leaf colors a*, b*, the hue angle, and total anthocyanin content showed the highest in QD-LED light, while b* and the hue angle values ​​were the lowest. As the light wavelength range was changed, there was a significant difference in the growth response and leaf color, and it is considered that QD-LED light should be considered positively when applied to plant factories.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Effects of Hydro and Hormonal Seed Priming on Seed Germination of Milk Thistle under Saline Stress Condition
Authors: NASIRI Yousef , FEYZI Parisa , JAVANMARD Abdollah 
Published: July 2019

Salinity is an abiotic stress which has harmful effects on germination of many plants. Therefore, high germination rate and vigorous early growth under salty soils is preferred. Seed priming is a way to increase salt tolerance of plants. An experiment was conducted to investigate the effect of seed priming on germination of milk thistle under salinity condition. The treatments were 4 levels of seed priming (no priming, distilled water as hydro priming and 0.5 and 1.0 mM salicylic acid) and 5 levels of salinity (0, 40 and 80 mM NaCl and 40 and 80 mM CaCl2). The experiment arranged as a factorial in a completely randomized design (CRD) with three replications. Results showed that salinity decreased germination percentage and germination rate to about 16 and 32% in 80 mM CaCl2 level compared to control, respectively. The highest mean germination time (5.7 day) were belonged to 80 mM CaCl2. Radicle and plumule length significantly decreased by 80 mM NaCl and 40 and 80 mM CaCl2. The lowest seedling weight and seed stamina observed in 80 mM CaCl2. 0.5 mM salicylic acid improved all traits except mean germination time as compared to control. Salicylic acid (0.5 mM) improved radicle length under 0, 40 and 80 mM NaCl salinity levels as well as increased plumule length at the 0 and 40 mM NaCl salinity conditions.
Number of Microorganisms in the Soil Cultivated with Legume-Grass Mixtures Supplied with Phytohormones and Nitrogen
Authors: SOSNOWSKI Jacek , JANKOWSKI Kazimierz , VIDICAN Roxana , WIŚNIEWSKA-KADŻAJAN Beata , MALINOWSKA Elżbieta , KACZOREK Anna 
Published: July 2019

The study concerned the number of microorganisms colonizing the humus layer and under plough soil from the cultivation of perennial ryegrass mixtures with red clover, which were fed by biostimulant based on the phytohormones and different doses of nitrogen. Nitrogen was applied at four levels-control (no nitrogen), 50, 100 and 150 kg N∙ha-1, biostimulant in two combinations - with and without preparation. Soil material to assess the size of individual groups of microorganisms was collected from each experimental plot in autumn 2010 from the humus (0-20 cm) and under plough layers (20-40 cm). The analysis of soil samples for a total number of bacteria, actinomycetes and fungi was conducted at the Department of Agricultural Microbiology IUNG-PIB in Pulawy. Results of studies have shown that more colonies of bacteria, actinomycetes and fungi were colonized the humus horizon. Biostimulant application in this cultivation led to a decrease of the total number of colonies of the all soil microorganisms groups but improved the ratio of bacteria to fungi. Increasing doses of mineral nitrogen by the limiting of the bacteria number and intensive development of soil fungi, helped to narrow the B/F ratio.
Anti-CD45RB Antibody Therapy Attenuates Renal Ischemia-Reperfusion Injury by Inducing Regulatory B Cells
Authors: Fang Taishi , Koo Tai Yeon , Lee Jae-Ghi , Jang Joon Young , Xu Yixuan , Hwang Ju Hee , Park Sunjoo , Yan Ji-Jing , Ryu Jung-Hwa , Ryu Yeon-Mi , Kim Sang-Yeob , Suh Kyung-Suk , Yang Jaeseok 
Published: July 2019

Significance Statement
The role of B cells in renal ischemia-reperfusion injury (IRI) remains controversial, and the role of the recently discovered B cell subset, regulatory B cells, in renal IRI has not yet been studied. The authors demonstrated in mouse models that regulatory B cells attenuated renal IRI. They also found that treatment with anti-CD45RB with or without anti–Tim-1, which induces regulatory B cells and suppresses T cells, attenuated acute renal injury when given before IRI and facilitated renal recovery when given after IRI. The main mechanism underlying the anti-CD45RB–mediated reno-protective effects was induction of IL-10+ regulatory B cells. These findings provide insight into the role of regulatory B cells in renal IRI and suggest that anti-CD45RB may be a potential therapeutic strategy in renal IRI.

Background
Regulatory B cells are a newly discovered B cell subset that suppresses immune responses. Recent studies found that both anti-CD45RB and anti–Tim-1 treatments regulate immune responses by inducing regulatory B cells; however, the role of these cells in renal ischemia-reperfusion injury (IRI) is unknown.

Methods
Using mouse models, including T cell–deficient (RAG1 knockout and TCRα knockout) mice and B cell–deficient (μMT) mice, we investigated the effects of regulatory B cells and anti-CD45RB on IRI and the mechanisms underlying these effects.

Results
Adoptive transfer of regulatory B cells before or after IRI attenuated renal IRI. Anti-CD45RB treatment with or without anti–Tim-1 before IRI increased renal infiltration of CD19+Tim-1+ regulatory B and regulatory T cells. Anti-CD45RB decreased serum creatinine levels, pathologic injury score, tubular apoptosis, and proinflammatory cytokines levels, whereas IL-10 levels increased. Following IRI, anti-CD45RB with or without anti–Tim-1 also induced regulatory B cells, improving renal function and tubular regeneration. In RAG1 knockout mice with B cell transfer, TCRα knockout mice, and wild-type mice with T cell depletion, anti-CD45RB increased regulatory B cells and attenuated IRI. However, anti-CD45RB did not attenuate IRI in RAG1 knockout mice with T cell transfer or μMT mice and induced only mild improvement in wild-type mice with B cell depletion. Furthermore, B cell–deficient mice receiving B cells from IL-10 knockout mice (but not from wild-type mice) did not show renal protection against IRI when treated with anti-CD45RB.

Conclusions
Anti-CD45RB treatment attenuated acute renal injury and facilitated renal recovery after IRI through induction of IL-10+ regulatory B cells, pointing to anti-CD45RB as a potential therapeutic strategy in renal IRI.