bluuebunny/crossref_metadata_2025_split huggingface dataset:
Murine Model of Maternal Immunization Demonstrates Protective Role for Antibodies That Mediate Antibody-Dependent Cellular Cytotoxicity in Protecting Neonates From Herpes Simplex Virus Type 1 and Type 2
Authors: Kao Carol M , Goymer Jessica , Loh Lip Nam , Mahant Aakash , Aschner Clare Burn , Herold Betsy C 
Published: October 2019

AbstractBackgroundNeonatal herpes simplex virus (HSV) disease results in unacceptable morbidity and mortality. The primary humoral immune response to natural infection is neutralizing antibodies (Abs). However, Abs that activate Fc gama receptors (FcγRs) and mediate antibody-dependent cell-mediated cytotoxicity (ADCC) may play a dominant role in protection. In adult mice, a single-cycle HSV candidate vaccine deleted in glycoprotein-D (ΔgD-2) that induces ADCC provided complete protection against HSV disease and prevented the establishment of latency. Passive transfer studies showed that Abs were sufficient for protection. The current study tested the hypothesis that maternal immunization with ΔgD-2 would protect neonates.MethodsC57BL/6 female mice were vaccinated 3 weeks apart with ΔgD-2, and pups were challenged at different times postnatally with lethal doses of HSV-1 or HSV-2. Concentration and functionality of Abs and immune cells were assessed.ResultsMaternal ΔgD-2 immunization provided significant protection and reduced viral dissemination after lethal challenge with HSV-1 or HSV-2. Protection correlated with Abs acquired transplacentally or from breastmilk that mediated ADCC. Protection was reduced when pups were challenged on Day 1 of life, and this was associated with decreased ability of newborn cells to mediate Ab-dependent cell killing.ConclusionsAntibodies mediating ADCC provide significant protection against neonatal HSV.
Effects of dietary energy level on appetite and central adenosine monophosphate-activated protein kinase (AMPK) in broilers
Authors: Hu Xiyi , Wang Yufeng , Sheikhahmadi Ardashir , Li Xianlei , Buyse Johan , Lin Hai , Song Zhigang 
Published: October 2019

Abstract
Adenosine monophosphate-activated protein kinase (AMPK) acts as a sensor of cellular energy changes and is involved in the control of food intake. A total of 216 1-d-old broilers were randomly allotted into 3 treatments with 6 replicates per treatment and 12 broilers in each cage. The dietary treatments included 1) high-energy (HE) diet (3,500 kcal/kg), 2) normal-energy (NE) diet (3,200 kcal/kg), and 3) low-energy (LE) diet (2,900 kcal/kg). The present study was conducted to investigate the effects of dietary energy level on appetite and the central AMPK signal pathway. The results showed that a HE diet increased average daily gain (ADG), whereas a LE diet had the opposite effect (P < 0.05, N = 6). The average daily feed intake (ADFI) of the chickens fed the LE diet was significantly higher than that of the control (P < 0.05, N = 6). Overall, the feed conversion rate gradually decreased with increasing dietary energy level (P < 0.05, N = 6). Moreover, the chickens fed the LE and HE diets demonstrated markedly improved urea content compared with the control group (P < 0.0001, N = 8). The triglyceride (TG) content in the LE group was obviously higher than that in the HE group but showed no change compared with the control (P = 0.0678, N = 8). The abdominal fat rate gradually increased with increased dietary energy level (P = 0.0927, N = 8). The HE group showed downregulated gene expression levels of liver kinase B1 (LKB1), neuropeptide Y (NPY), cholecystokinin (CCK), and glucocorticoid receptor (GR) in the hypothalamus compared with the control group (P < 0.05, N = 8). However, LE treatment significantly increased the mRNA level of AMP-activated protein kinase α2 (AMPKα2) compared with other groups (P = 0.0110, N = 8). In conclusion, a HE diet inhibited appetite and central AMPK signaling. In contrast, a LE diet activated central AMPK and appetite. Overall, the central AMPK signal pathway and appetite were modulated in accordance with the energy level in the diet to regulate nutritional status and maintain energy homeostasis in birds.
Purification, biochemical and molecular study of lipase producing from a newly thermoalkaliphilic Aeribacillus pallidus for oily wastewater treatment
Authors: Ktata Ameni , Krayem Najeh , Aloulou Ahmed , Bezzine Sofiane , Sayari Adel , Chamkha Mohamed , Karray Aida 
Published: October 2019

AbstractTreatment of oily wastewater is constantly a challenge; biological wastewater treatment is an effective, cheap and eco-friendly technology. A newly thermostable, haloalkaline, solvent tolerant and non-induced lipase from Aeribacillus pallidus designated as GPL was purified and characterized of biochemical and molecular study for apply in wastewater treatment. The GPL showed a maximum activity at 65°C and pH 10 after 22 h of incubation, with preference to TC4 substrates. Pure enzyme was picked up after one chromatographic step. It displayed an important resistance at high temperature, pH, NaCl, at the presence of detergents and organic solvents. In fact, GPL exhibited a prominent stability in wide range of organic solvents at 50% (v/v) concentration for 2 h of incubation. The efficiency of the GPL in oil wastewater hydrolysis was established at 50°C for 1 h, the oil removal efficiency was established at 96, 11% and the oil biodegradation was confirmed through fourier transform infrared (FT-IR) spectroscopy. The gene that codes for this lipase was cloned and sequenced and its open reading frame encoded 236 amino acid residues. The deduced amino acids sequence of the GPL shows an important level of identity with Geobacillus lipases.
The Effect of Entomopathogenic Fungi on Enzymatic Activity in Chlorpyrifos-Resistant Mosquitoes, Culex quinquefasciatus (Diptera: Culicidae)
Authors: Ismail Haji Muhammad , Freed Shoaib , Naeem Afifa , Malik Shahjahan , Ali Najaf 
Published: October 2019

Abstract
Culex quinquefasciatus Say is an important pest species and a vector of multiple pathogens. Insecticide applications are necessary for the effective control of mosquitoes. In the current study, a laboratory population of Cx. quinquefasciatus was exposed to chlorpyrifos for 15 consecutive generations and then assessed for the changes in detoxification enzyme activities before and after exposure to Metarhizium anisopliae (Metschn.) Sorokin and Beauveria bassiana (Bals.) Vuill. during 14th–15th generations. Activities of acetylcholinesterase (AChE), glutathione S-transferase (GST), esterase (EST), acid phosphatases (ACP), and alkaline phosphatases (ALP) were increased in the chlorpyrifos-selected (Chlor-SEL) population in relation to an unselected (Un-SEL) population. The resistance ratio of Chlor-SEL 15th generation (G15) was increased 3,583-fold against first generation (G1) and 6,026-fold against the Un-SEL population. The results depicted maximum activities of ACP (83.48), ALP (65.54), GST (13.047), EST (10.42), and AChE (4.86) μmol/min of mg/ml protein at G15 after consecutive chlorpyrifos applications. The Chlor-SEL populations at G14–G15 were treated with different concentrations of M. anisopliae and B. bassiana for possible suppression of enzymatic activities. Activities of ACP were suppressed to 24.22 μmol/min of mg/ml protein at G15 when exposed to B. bassiana and 22.40 μmol/min of mg/ml protein at G14 after exposure to M. anisopliae. The suppression of detoxification enzymes by application of fungi in resistant population of Cx. quinquefasciatus will aid in the mosquito’s management programs.
Olfactometric Comparison of the Volatile Insecticide, Metofluthrin, Through Behavioral Responses of Aedes albopictus (Diptera: Culicidae)
Authors: Bibbs Christopher S , Kline Jedidiah , Kline Daniel L , Estaver Jim , Strohschein Rudolph , Allan Sandra A , Kaufman Phillip E , Xue Rui-De , Batich Christopher D 
Published: October 2019

Abstract
Testing behavioral response to insecticidal volatiles requires modifications to the existing designs of olfactometers. To create a testing apparatus in which there is no chemical memory to confound tests, we detail the technical aspects of a new tool with design influences from other olfactometry tools. In addition, this new tool was used to evaluate a novel formulation of metofluthrin for use as an outdoor residual treatment. After sourcing materials to prioritize glass and metal construction, a modular wind tunnel was developed that hybridizes wind tunnel and olfactometer specifications. Volatile contaminants were removed by strong ultraviolet light within the chamber before and between trials. Repellent trials were conducted with an experimental formulation of metofluthrin and a commercial formulation of esfenvalerate, prallethrin, and piperonyl butoxide (Onslaught Fast Cap) against Aedes albopictus (Skuse). Toxicant vapors were delivered with attractants from a lure with behavioral responses scored 20 min post-exposure. Upwind attraction to the attractant lure and the Onslaught Fast Cap plus lure resulted in 90 and 75% capture, respectively. In contrast, metofluthrin vapors resulted in less than 10% attraction, while also causing repellency, disorientation, knockdown, and mortality effects. Our findings demonstrated that an inert modular wind tunnel was functional for mitigating toxic secondary exposures of spatial repellents amidst complex behavioral analysis in mosquitoes. The resulting observations with formulated metofluthrin positively reinforce the merit of transitioning metofluthrin into expanded roles in mosquito management.
Rapid Peptides Generator: fast and efficient in silico protein digestion
Authors: Maillet Nicolas 
Published: October 2019

AbstractRecent developments in mass spectrometry techniques used in proteomics and proteogenomics have led to a constantly increasing interest in proteases. These proteases are used in different mass spectrometry analyses requiring protein digestions. To perform such digestions, one or multiple proteases are used. Few software exist that predict cleavage sites of proteases and simulate in silico digestions. In this work, Rapid Peptides Generator (RPG), a new software developed in order to predict proteases-induced cleavage sites on sequences, is presented. RPG offers extra features and overcomes most issues of existing software in different ways. First, for each generated peptide, RPG gives its sequence, length and estimation of mass, measurements already provided by other software, as well as the peptide’s isoelectric point. Moreover, contrary to existing software that limit the option of proteases to be used to a predefined list, users of RPG are able to easily define new proteases using a simple yet powerful grammar. This feature allows users to stay up-to-date to new or more specific proteases available on the market and optimizes time and effort before the actual mass spectrometry experiment. RPG is freely available through the well established package management system ‘pip’ and follows the standards for software development.
Robotic Surgical Assistant (ROSA™) Rehearsal: Using 3-Dimensional Printing Technology to Facilitate the Introduction of Stereotactic Robotic Neurosurgical Equipment
Authors: Bonda David J , Pruitt Rachel , Goldstein Todd , Varghese Anish , Mittler Mark , Schneider Steven , Shah Amar , Rodgers Shaun 
Published: October 2019

Abstract

BACKGROUND
The use of frameless stereotactic robotic technology has rapidly expanded since the Food and Drug Administration's approval of the Robotic Surgical Assistant (ROSA™) in 2012. Although the safety and accuracy of the ROSA platform has been well-established, the introduction of complex robotic technology into an existing surgical practice poses technical and logistical challenges particular to a given institution.

OBJECTIVES
To better facilitate the integration of new surgical equipment into the armamentarium of a thriving pediatric neurosurgery practice by describing the use of a three-dimensional (3D)-printed patient model with in situ 3D-printed tumor for presurgical positioning and trajectory optimization in the stereotactic biopsy of a pontine lesion in a pediatric patient.

METHODS
A 3D model was created with an added silicone mock tumor at the anatomical position of the lesion. In a preoperative rehearsal session, the patient model was pinned and registered using the ROSA platform, and a mock biopsy was performed targeting the in Situ silicone tumor.

RESULTS
Utilization of the 3D-printed model enabled workflow optimization and increased staff familiarity with the logistics of the robotic technology. Biopsy trajectory successfully reached intralesional tissue on the 3D-printed model. The rehearsal maneuvers decreased operative and intubation time for the patient and improved operative staff familiarity with the robotic setup.

CONCLUSION
Use of a 3D-printed patient model enhanced presurgical positioning and trajectory planning in the biopsy of a difficult to reach pontine lesion in a pediatric patient. The ROSA rehearsal decreased operative time and increased staff familiarity with a new complex surgical equipment.
Risks of suicide attempts after prescription of zolpidem in people with depression: a nationwide population study in South Korea
Authors: Kim Hyewon , Kim Yuwon , Myung Woojae , Fava Maurizio , Mischoulon David , Lee Unjoo , Lee Hyosang , Jin Na Eun , Woo Choi Kwan , Shin Myung-Hee , Jin Jeon Hong 
Published: October 2019

AbstractObjectivesTo investigate the association between zolpidem prescription and suicide attempts in people with depressionMethodsA nationwide, population-based electronic medical records database from the Health Insurance Review & Assessment Service of South was used to investigate the incidence rate ratios (IRRs) of suicide attempts and probable suicide attempts in people with depression before and after zolpidem prescription using self-controlled case series design.ResultsIn a total of 445 people who attempted suicide and 23 141 people who attempted probable suicide attempt, the IRRs of suicidal behavior during the risk periods before and after zolpidem prescription increased compared with those at the baseline. The IRRs gradually increased and peaked immediately before the prescription of zolpidem. The IRR was 70.06 (95% CI: 25.58–191.90) on day 2 before zolpidem prescription and 63.35 (95% CI: 22.99–174.59) on day 1 after zolpidem prescription in the suicide attempt group. The IRR was 24.07 (95% CI: 20.50–28.26) on the day before zolpidem prescription and 14.96 (95% CI: 12.21–18.34) on the day after zolpidem prescription in the probable suicide attempt group. The ratios declined eventually after zolpidem was prescribed.ConclusionsAlthough zolpidem prescription was associated with an increased risk of suicide attempts in people with depression, the risk increased and peaked immediately before zolpidem prescription. The risk declined gradually thereafter. This result indicates that the risk of suicide attempts increases at the time of zolpidem prescription. However, zolpidem prescription does not contribute to additional increase in the risk of suicide attempts.
Oscillating and static dietary crude protein supply: II. Energy and protein requirements of young Nellore bulls
Authors: Menezes Ana Clara B , V. Filho Sebastião C , Pucetti Pauliane , C. Pacheco Marcos V , Godoi Letícia A , Zanetti Diego , Alhadas Herlon M , Paulino Mário F , Caton Joel S 
Published: October 2019

Abstract:
The objective of this study was to evaluate whole body chemical composition and energy and protein nutrient requirements for maintenance and gain of Nellore bulls. Fifty young bulls, with an average age of 7 ± 1 mo and initial body weight (BW) of 260.0 ± 8.1 kg, were used in this experiment. Four bulls were used as baseline reference animals and were slaughtered at the beginning of the experiment. Four bulls were fed at maintenance (12 g dry matter [DM]/kg of BW), whereas 42 bulls were divided into six groups (n = 7/group) and were randomly assigned to the following dietary treatments 105 (low [LO]), 125 (medium [MD]), or 145 (high [HI]) g crude protein (CP)/kg DM, LO to HI (LH), LO to MD (LM), or MD to HI (MH) oscillating CP at a 48-h interval for 140 d. At the end of the experiment, bulls were slaughtered and samples of the whole body were collected. All samples were lyophilized, ground, and composed as percentage of component of empty body weight (EBW) from each bull. A power model was used to estimate carcass, non-carcass components, and gastrointestinal content of the shrunk body weight (SBW), and CP and water present in the empty body, whereas an exponential model was used to estimate adipose tissue and ether extract (EE) present in the EBW. Nonlinear regression equations were developed to predict heat production from metabolizable energy (ME) intake and retained energy (RE). The net energy requirements for maintenance and ME for maintenance were 77 and 122.75 kcal/EBW0.75/d, respectively. The efficiency of ME utilization for maintenance was 62.7%. The equation obtained for net energy for gain (NEg) was: NEg (Mcal/EBW0.75/d) = 0.0535 × EBW0.75 × EBG0.7131, where EBG is the empty body gain, and the efficiency was 24.25%. Net protein requirement for growth (NPg) was: NPg (g/d) = 227.372 × EBG – 19.479 × RE. There was a linear increase for carcass, CP, and water present in the EBW as the animal grew. The EE deposition exponentially increased as EBW increased.
A web-based intervention to increase weight loss treatment initiation: results of a cluster randomized feasibility and acceptability trial
Authors: McVay Megan A , Yancy William S , Bennett Gary G , Levine Erica , Jung Seung-Hye , Jung Soyeon , Anton Steve , Voils Corrine I 
Published: October 2019

Abstract
Evidence-based behavioral weight loss treatment is under-utilized. To increase initiation of treatment, we developed a single-session, online, primary care-based intervention (“mobilization tool”). We evaluated the mobilization tool's acceptability for primary care patients with obesity, trial design feasibility, and signal of an effect of the tool on treatment initiation. In this cluster randomized feasibility trial, primary care providers (PCPs) were randomized to a mobilization tool or comparator tool arm. Patients with obesity and a scheduled appointment with a randomized PCP were assigned to complete the mobilization or comparator tool prior to their appointment. The online mobilization tool asks patients to answer questions about a variety of weight-related topics and then provides automated, tailored feedback that addresses psychosocial determinants of weight loss treatment initiation. The comparator tool provided a nontailored description of treatments. All participants were offered free enrollment in behavioral weight loss treatments. Six PCPs were randomized. Sixty patients (57% female; 66% white; aged 55 ± 13 years) participated in this study of 296 contacted for eligibility evaluation (20.2%). Six-month follow-up assessments were completed by 65% (22/34) of the mobilization and 73% (19/26) of comparator tool participants. Participants completing the acceptability survey reported that the mobilization tool was usable, enjoyable, informative, and useful. Weight loss treatment was initiated by 59% (n = 19) of mobilization and 33% (n = 8) of comparator tool participants. The mobilization tool shows promise for increasing treatment initiation among primary care patients, which may increase population weight loss.
Trial Registration: Clinicaltrials.gov identifier: NCT02708121.
Postgraduate education among family and community physicians in Brazil: theTrajetórias MFCproject
Authors: Fontenelle Leonardo Ferreira , Rossi Stephani Vogt , de Oliveira Miguel Henrique Moraes , Brandão Diego José , Sarti Thiago Dias 
Published: October 2019

AbstractNeither primary health care or family and community medicine are recognized as knowledge areas in Brazil, for the purpose of postgraduate education (master’s, Ph.D.) or research. Our objective was to describe the postgraduate education trajectories of family and community physicians in Brazil. In this observational, exploratory study, we used data from SBMFC and SisCNRM to compile the list of physicians and community physicians, and then downloaded their curricula vitae from the Lattes Platform, verifying all data for consistency. A master’s degree was held by one in eight, and a Ph.D., by one in forty; most degrees were in collective health. Women (versus men) were less likely to hold master’s degrees, and even less likely to hold Ph.D. degrees. Professional (versus academic) master’s degrees and those in other areas (versus in medicine or collective health) were also associated with lower probability of obtaining a Ph.D. degree. Certified specialists (versus those with a medical residency) with a postgraduate degree were more likely to have earned it before becoming family and community physicians. We suggest that researchers in public health critically examine the relative benefits of different postgraduate trajectories for the professional performance of family and community physicians.
BAck Complaints in the Elders - Chiropractic (BACE-C): Design of a cohort study in chiropractic care
Authors: Jenks Alan D. , Hoekstra Trynke , Axén Iben , de Luca Katie , Field Jonathan , Newell Dave , Hartvigsen Jan , French Simon D. , Koes Bart , van Tulder Maurits W. , Rubinstein Sidney M. 
Published: October 2019

AbstractBackgroundLow back pain is a common condition among older adults that significantly influences physical function and participation. Compared to their younger counterparts, there is limited information available about the clinical course of low back pain in older people, in particularly those presenting for chiropractic care. Improving our understanding of this patient population and the course of their low back pain may provide input for studies researching safer and more effective care than is currently provided.ObjectivesThe primary objectives are to examine the clinical course over one year of the intensity, healthcare costs and improvement rates of low back pain in people 55 years and older who visit a chiropractor for a new episode of low back pain.MethodsAn international prospective, multi-center cohort study with one-year follow-up. Chiropractic practices are to be recruited in the Netherlands, Sweden, United Kingdom and Australia. Treatment will be left to the discretion of the chiropractor. Inclusion/Exclusion criteria: Patients 55 years and older who are accepted for care having presented to a chiropractor with a new episode of low back pain and who have not been to a chiropractor in the previous six months for an episode of low back pain are to be included, independent of whether or not they have seen another type of health care provider. Patients who are unable to complete the web-based questionnaires because of language restrictions or those with computer literacy restrictions will be excluded as well as those with cognitive disorders. In addition, those with a suspected tumor, fracture, infection or any other potential red flag or condition considered to be a contraindication for chiropractic care will be excluded. Data will be collected using online questionnaires at baseline, and at 2 and 6 weeks and at 3, 6, 9 and 12 months.Trial RegistrationNederlandse Trial Registrar NL7507