bluuebunny/crossref_metadata_2025_split huggingface dataset:
Host Plant Recognition and Performance of Klambothrips myopori (Thysanoptera: Phlaeothripidae) Across Myoporum Cultivars in Southern California
Authors: Shogren Christopher J , Paine Timothy D 
Published: March 2019

Abstract
Myoporum spp. (Lamiales: Scrophulariaceae) native to Australia and New Zealand have been introduced into North America and have been widely planted as shrubs and ground covers in the California urban landscape. The invasive thrips, Klambothrips myopori Mound and Morris, invaded California in 2005 and feed exclusively on Myoporum. However, some commercial cultivars have suffered extensive damage, whereas others have not. In field studies and laboratory feeding tests of six different potential host species or cultivars, Myoporum laetum and Myoporum ‘Pacificum’ were identified as most suitable host plants of K. myopori. In laboratory trials, K. myopori failed to complete development on M. ‘Clean n Green’ and M. ‘Putah Creek’. Of the six varieties of Myoporum tested, K. myopori damage was only observed on M. laetum and M. ‘Pacificum’. Although K. myopori can successfully colonize and reproduce on several varieties of Myoporum, they demonstrated a preference for M. laetum and M. ‘Pacificum’ in laboratory and field trials.
Clinical and Molecular Characterization of Adult Glioblastomas in Southern Brazil
Authors: Trevisan Patricia , Graziadio Carla , Rodrigues Dayane Bohn Koshiyama , Rosa Rafael Fabiano Machado , Soares Fabiano Pasqualotto , Provenzi Valentina Oliveira , de Oliveira Ceres Andréia Vieira , Paskulin Giorgio Adriano , Varella-Garcia Marileila , Zen Paulo Ricardo Gazzola 
Published: March 2019

AbstractWe investigated 113 adult Brazilian patients with glioblastoma (GBM) for comparison with patients from distinct geographical areas and evaluation of suitability for novel targeted therapies. Patients were assessed for clinical features and tumor genomic characteristics such as ROS1 and NTRK1 rearrangements, KIT, PDGFRA, and KDR amplification, and RB1 deletion using multicolor fluorescence in situ hybridization. The majority of patients were male (53%), over 40 years (94%), with tumor located in single site (64%), in the right cerebral hemisphere (60%), and underwent partial resection (71%); 14% presented complications after surgery. The main clinical sign at diagnosis was focal abnormality (57%); frontal (31%); and temporal (20%) regions were most commonly affected. Median hospitalization time was 20 days, median survival was 175 days. One tumor was positive for rearrangement in NTRK1 and another in ROS1 (0.9% each). PDGFRA was amplified in 20% of cases, often co-amplified with KDR (&gt;90%) and KIT (&gt;60%). RB1 was deleted in 16% of patients. There was no association between these molecular abnormalities and patient survival. However, older age, complications after surgery, and right-sided tumors were independent variables associated with patient survival. This study contributes information on the molecular profile of glioblastomas in Latin America possibly supporting new target therapies.
Cost-Efficiency of Breast Cancer Care in the US Military Health System: An Economic Evaluation in Direct and Purchased Care
Authors: Eaglehouse Yvonne L , Georg Matthew W , Richard Patrick , Shriver Craig D , Zhu Kangmin 
Published: March 2019

Abstract

Introduction
With the rising costs of cancer care, it is critical to evaluate the overall cost-efficiency of care in real-world settings. In the United States, breast cancer accounts for the largest portion of cancer care spending due to high incidence and prevalence. The purpose of this study is to assess the relationship between breast cancer costs in the first 6 months after diagnosis and clinical outcomes by care source (direct or purchased) in the universal-access US Military Health System (MHS).

Materials and Methods
We conducted a retrospective analysis of data from the Department of Defense Central Cancer Registry and MHS Data Repository administrative records. The institutional review boards of the Walter Reed National Military Medical Center and the Defense Health Agency reviewed and approved the data linkage. We used the linked data to identify women aged 40–64 who were diagnosed with pathologically-confirmed breast cancer between 2003 and 2007 with at least 1 year of follow-up through December 31, 2008. We identified cancer treatment from administrative data using relevant medical procedure and billing codes and extracted costs paid by the MHS for each claim. Multivariable Cox proportional hazards models estimated hazards ratios (HR) and 95% confidence intervals (CI) for recurrence or all-cause death as a function of breast cancer cost in tertiles.

Results
The median cost per patient (n = 2,490) for cancer care was $16,741 (interquartile range $9,268, $28,742) in the first 6 months after diagnosis. In direct care, women in the highest cost tertile had a lower risk for clinical outcomes compared to women in the lowest cost tertile (HR 0.58, 95% CI 0.35, 0.96). When outcomes were evaluated separately, there was a statistically significant inverse association between higher cost and risk of death (p-trend = 0.025) for women receiving direct care. These associations were not observed among women using purchased care or both care sources.

Conclusions
In the MHS, higher breast cancer costs in the first 6 months after diagnosis were associated with lower risk for clinical outcomes in direct care, but not in purchased care. Organizational, institutional, and provider-level factors may contribute to the observed differences by care source. Replication of our findings in breast and other tumor sites may have implications for informing cancer care financing and value-based reimbursement policy.
Development and evaluation of a real-time polymerase chain reaction for fast diagnosis of sporotrichosis caused by Sporothrix globosa
Authors: Zhang Mingrui , Li Fuqiu , Gong Jie , Yang Xin , Zhang Jianzhong , Zhao Fei 
Published: March 2019

Abstract
Sporothrix globosa is an important clinical pathogen in the Sporothrix complex, which is causing sporotrichosis. S. globosa is distributed worldwide, especially in Asia. The transmission medium of S. globosa is mainly contaminated soil or decaying vegetation, and the infection usually caused by transcutaneous trauma, through which the fungal conidia or yeast cells enter the host. Although the clinical manifestations of sporotrichosis caused by S. globosa is always benign, there have been several outbreaks worldwide. In this study, we established a novel real-time polymerase chain reaction (PCR) method based on the internal transcribed spacer (ITS) sequence for the identification of S. globosa. The assay was further evaluated by clinical specimens obtained from patients of sporotrichosis. The sensitivity and specificity of the real-time PCR method was both 100%. The detection limit was 10 fg. The positive detection rate for 30 clinical specimens, which were confirmed infected by S. globosa, was 100%. The real-time PCR method established in this paper is a rapid, sensitive and specific method for the identification of S. globosa. It can detect S. globosa in clinical specimen from patients with sporotrichosis, which is helpful for fast clinical diagnosis.
CHARACTERISTICS OF TYPE 3 NEOVASCULARIZATION LESIONS
Authors: Kim Jae Hui , Chang Young Suk , Kim Jong Woo , Kim Chul Gu , Lee Dong Won 
Published: March 2019

Purpose:
To evaluate the incidence of multifocal lesions and the distribution of lesion location in Type 3 neovascularization.

Methods:
This retrospective, observational study included 148 eyes of 148 patients diagnosed with Type 3 neovascularization. The number of Type 3 neovascularization lesions was counted, and the incidence of multiple lesions in an eye was estimated. In addition, the distance from the fovea to the lesion and the geographic location of the lesion were estimated. Pseudodrusen incidence was compared between eyes with and without multifocal lesions.

Results:
In total, 169 Type 3 neovascularization lesions were noted. A single lesion was noted in 130 eyes (87.8%), whereas 2 or 3 multifocal lesions were noted in the remaining 18 eyes (12.2%). The mean distance from the fovea to the lesion was 898.8 ± 324.9 µm. The distribution of lesion locations exhibited a fovea-sparing pattern. No lesions were located within 200 µm of the fovea, 20 lesions (11.8%) were located >200 and ≤500 µm away from the fovea, 89 lesions (52.7%) were located >500 and ≤1,000 µm away from the fovea, and 60 lesions (35.5%) were located >1,000 µm away from the fovea. Pseudodrusen incidence was significantly higher in eyes with multifocal lesions (P = 0.024).

Conclusion:
Two or more multifocal lesions were noted in 12.2% of eyes with Type 3 neovascularization, and pseudodrusen incidence was higher in eyes with multifocal lesions. In addition, lesion distribution exhibited a fovea-sparing pattern. These characteristics may be associated with the distinct pathophysiology of Type 3 neovascularization.
What is new in electronic-cigarettes research?
Authors: Jenssen Brian P. , Wilson Karen M. 
Published: March 2019

Purpose of review
Electronic cigarettes (e-cigarettes) and other vaping devices have seen extraordinary growth in use in the past 10 years, and companies are accelerating their development of new products and marketing efforts. In turn, researchers have increased their efforts to determine how e-cigarettes affect health, how marketing these products impacts adolescents and how the use of e-cigarettes may affect adolescents’ use of other tobacco products. Products like Juul were not on the market 2 years ago; thus, frequent updates on the topic are essential.

Recent findings
Studies have begun to demonstrate that users of the newer pod systems are exposed to high levels of nicotine, as well as other chemicals. These products are highly marketed, with a strong emphasis on how adolescents can use them surreptitiously. This is concerning to researchers, and other studies have continued to demonstrate that teens who use e-cigarettes are more likely to go on to use combusted tobacco. Further research has also failed to demonstrate that e-cigarettes are useful for people wishing to quit smoking combusted tobacco.

Summary
E-cigarettes and vaping systems are not safe products and should not be used by adolescents.
Gamma probe-guided confirmation of balloon placement in endovascular procedures
Authors: Park Yunchul , Jo Young Goun , Choi Kang-Ho , Kim Munki , Kim Jahae 
Published: March 2019

BACKGROUND
Gamma probes can provide real-time information on lesion location and allow radioguided surgery in many diseases. The purpose of this study was to determine whether a gamma probe could be used to confirm balloon location in resuscitative endovascular balloon occlusion of the aorta and to determine the ease-of-use of the gamma probe localization method using human vasculature phantom and large animal.

METHODS
This investigation comprised a proof-of-concept study using human vasculature phantom and feasibility study using large animal. An assistant and two general surgeons with no prior experience in handling a gamma probe participated in both studies. A gamma probe was used by each surgeon to explore the location of a catheter balloon filled with 8 mL of normal saline containing 37 MBq of 99mTechnetium. The differences between predicted and actual balloon locations and the times taken to perform the trials were recorded. Balloon locations were confirmed by planar gamma camera images. The results were analyzed and compared according to the operators and experiments.

RESULTS
Overall sensitivity and specificity for the localization of the catheter balloon were 100% and 91% in phantom study, respectively, and 100% and 100% in animal study, respectively. The performance of the two operators for exploring the balloon location was similar in both the phantom and the animal studies. The mean time taken to explore the balloon locations with the aid of the gamma probe was only 2.36 minutes in phantom study and 2.65 minutes in the animal study.

CONCLUSION
The gamma probe method was accurate and rapid for confirming balloon location. We suggest that the gamma probe has potential for confirming balloon placement in endovascular procedures without the need for fluoroscopy.
Red blood cell storage and adhesion to vascular endothelium under normal or stress conditions: An in vitro microfluidic study
Authors: Diebel Lawrence N. , Liberati David M. 
Published: March 2019

BACKGROUNDObservational studies have identified an association between duration of red blood cell (RBC) storage and adverse outcomes in trauma. Hemorrhagic shock (HS) leads to impaired tissue perfusion which is associated with endothelial cell glycocalyx (eGC) shedding. Adhesion of stored RBC to the vascular endothelium has been shown to lead to impaired perfusion in the microcirculation and contribute to organ failure and poor outcome. The role of either or both of the EC and RBC glycocalyx in this process is unknown and was studied in anin vitromodel.METHODSHuman umbilical vein endothelial cells were perfused in a microfluidic device with RBC solutions from fresh, less than 14-day or longer than 21-day storage. In some experiments, the HS microenvironment was simulated by hypoxia-reoxygenation (H/R) and epinephrine (Epi) in the perfusion experiments. Measurements obtained included endothelial cell (EC) and RBC glycocalyx and RBC adherence to human umbilical vein endothelial cell monolayers at variable shear rates.RESULTSEndothelial cell glycocalyx and RBC glycocalyx dimensions were reduced by H/R and Epi and storage duration respectively. Red blood cell adherence to the endothelium was increased by H/R + Epi treatment and duration of RBC storage.CONCLUSIONOur data may help explain some of the remaining discrepancies regarding the impact of RBC storage duration on outcomes in the trauma population. Consideration of the integrity of the EC and RBC glycocalyx may guide future transfusion strategies in the trauma population. The microfluidic device system platform may offer a high throughput modality to study emerging therapies to mitigate adverse consequence of RBC storage duration on the perfused endothelium in the trauma setting.
ThoraSite: A device to improve accuracy of lateral decompression needle and chest tube placement
Authors: Shah Amit Navin , Kothera Curt Steven , Dheer Sachin 
Published: March 2019

BACKGROUND
Multiple reports have detailed an unacceptably high error rate in the siting of decompression needles and tubes and describe associated iatrogenic injuries. The objective of the current study was to measure the accuracy of the novel ThoraSite template for identifying an acceptable intercostal space (ICS) for lateral needle or tube thoracostomy.

METHODS
Two trained operators used the ThoraSite to place radiopaque needles in the left and right lateral chests of 12 cadavers. An independent radiologist reviewed fluoroscopy images to determine the primary outcome: the ICS in which each needle was placed. Secondary outcomes were ICS's palpable through ThoraSite's Safe Zone; needle placement relative to the anterior axillary line (AAL) and midaxillary line (MAL); and percent correct placement (defined as the third, fourth, or fifth ICS from 1 cm anterior to the AAL to 1 cm posterior to the MAL).

RESULTS
The six female and six male cadavers spanned 4 ft and 11 inches (150 cm) to 6 ft and 7 inches (201 cm), 80 lb (36 kg) to 350 lb (159 kg), and 16 kg/m2 to 42 kg/m2 body mass index. All 24 needles were placed in either the third (4 [17%] of 24 needles), fourth (10 [42%] of 24 needles), or fifth ICS (10 [42%] of 24 needles). In 10 (42%) of 24 assessments, two ICSs were palpable in ThoraSite's Safe Zone. All palpable ICSs were either the third (8 [24%] of 34), fourth (15 [44%] of 34); or fifth ICS (11 [32%] of 34). Twenty-three (96%) of 24 needles were inserted from 1 cm anterior to the AAL to 1 cm posterior to the MAL. Twenty-three (96%) of 24 needle placements were correct.

CONCLUSION
ThoraSite use was associated with needle placement in the third, fourth, or fifth ICS in an area roughly spanning the AAL to MAL in anatomically diverse cadavers. By facilitating appropriate needle/tube placement, ThoraSite use may decrease iatrogenic injuries. Future study involving representative users may be useful to further evaluate ThoraSite accuracy.

LEVEL OF EVIDENCE
Therapeutic and care management, level IV.
Repeated Transcranial Magnetic Stimulation–Induced Motor Evoked Potentials Correlate With the Subject-Specific Serum Metabolic Profile of Creatine
Authors: Kallioniemi Elisa , Kärkkäinen Olli , Määttä Sara , Könönen Mervi , Kivimäki Petri , Kaarre Outi , Velagapudi Vidya , Kekkonen Virve , Lehto Soili M. , Laukkanen Eila , Tolmunen Tommi 
Published: March 2019

Purpose:
Transcranial magnetic stimulation–induced motor responses have been considered to mainly reflect the electrophysiological characteristics of the central motor system. However, certain motor phenomena, such as the magnitude of repetition suppression measured with motor evoked potentials (MEPs), could also in part be influenced by metabolic processes in the peripheral muscles and in both the peripheral and central nervous system. Repetition suppression is an inhibitory phenomenon in which the amplitude of MEP decreases in comparison to that of the first MEP in a train of transcranial magnetic stimulation pulses. This study aimed to identify possible metabolic processes influencing repetition suppression.

Methods:
The metabolic profiles from serum samples and repetition suppression from the right abductor pollicis brevis muscle were measured in 73 subjects (37 female subjects). Repetition suppression was measured using trains of transcranial magnetic stimulation stimuli consisting of 4 identical single pulses at 1-second intervals. The trains were repeated every 20 seconds, and 30 trains were given with a stimulation intensity of 120% of the resting motor threshold of the abductor pollicis brevis. Thus, a total of 120 stimuli were administered.

Results:
The main finding was a significant negative relationship between serum creatine levels and the magnitude of repetition suppression (standardized β coefficient (β) = −0.43; P < 0.001). In other words, higher creatine levels corresponded to a smaller decrement in the MEP amplitude in response to repetition. When MEPs were not repeated, no relationship was observed (β = 0.09; P = 0.454). Creatine is used to form phosphocreatine, which in turn is needed to resynthesize adenosine triphosphate from adenosine diphosphate in situations requiring high amounts of energy in muscles and neural cells.

Conclusions:
For the first time, this study demonstrated a connection between repeated MEPs and peripheral serum metabolites linked to muscle function. These findings could explain some of the intersubject variability commonly observed in MEPs when the pulses are repeated.
Risk of multiple primary cancer diagnosis over age in families of Li-Fraumeni syndrome: a single institution perspective
Authors: Shin Seung Jun , Dodd Elissa B. , Gao Fan , Bojadzieva Jasmina , Chen Jingxiao , Kong Xianhua , Amos Chris , Ning Jing , Strong Louise C. , Wang Wenyi 
Published: March 2019

AbstractLi-Fraumeni syndrome (LFS) is a rare autosomal dominant disorder associated with TP53 germline mutations and an increased lifetime risk of multiple primary cancers (MPC). Penetrance estimation of time to first and second primary cancer within LFS remains challenging due to limited data availability and the difficulty of accounting for the characteristic effects of a primary cancer on the penetrance of a second primary cancer. Using a recurrent events survival modeling approach, we estimated penetrance for both first and second primary cancer diagnosis from a pediatric sarcoma cohort (number families=189; Single primary cancer, or SPC=771; MPC=87). We then validated the risk prediction performance using an independent clinical cohort of TP53 tested individuals from MD Anderson (SPC=102; MPC=58). Our penetrance estimates are dependent on TP53 genotype, sex, and, importantly, age of diagnosis (AoD) for the first PC. We observed the later the AoD is, the shorter the gap time is for this person to be diagnosed with a second PC. We achieved an Area under the ROC curve (AUC) of 0.77 for predicting individual outcomes of MPC vs. SPC. In summary, we have provided the first set of penetrance estimates for SPC and MPC for TP53 mutation carriers, and demonstrated its accuracy for cancer risk assessment. Our open-source R package, LFSPRO, provides future risk estimates for SPC and MPC among TP53 germline mutation carriers.SignificanceOur tool can be used to support clinical counseling of LFS cancer survivors for better health management.
Pacbio sequencing reveals identical organelle genomes between American cranberry (Vaccinium macrocarponAit.) and a wild relative
Authors: Diaz-Garcia Luis , Rodriguez-Bonilla Lorraine , Smith Tyler , Zalapa Juan 
Published: March 2019

AbstractBreeding efforts in the American cranberry (Vaccinium macrocarpon Ait.), a North American perennial fruit crop of great importance, have been hampered by the limited genetic and phenotypic variability observed among cultivars and experimental materials. Most of the cultivars commercially used by cranberry growers today were derived from a few wild accessions bred in the 1950s. In different crops, wild germplasm has been used as an important genetic resource to incorporate novel traits and increase the phenotypic diversity of breeding materials.Vaccinium microcarpum(Turcz. ex Rupr.) Schmalh. andV. oxycoccosL., two closely related species, may be cross-compatible with the American cranberry and could be useful to improve fruit quality such as phytochemical content, and given their northern distribution, could also help develop cold hardy cultivars. Although these species have previously been analyzed in diversity studies, genomic characterization and comparative studies are still lacking. In this study, we sequenced and assembled the organelle genomes of the cultivated American cranberry and its wild relative,V. microcarpum. PacBio sequencing technology allowed us to assemble both mitochondrial and plastid genomes at very high coverage and in a single circular scaffold. A comparative analysis revealed that the mitochondrial genome sequences were identical between both species and that the plastids presented only two synonymous single nucleotide polymorphisms (SNPs). Moreover, Illumina resequencing of additional accessions ofV. microcarpumandV. oxycoccosrevealed high genetic variation in both species. Based on these results, we provided a hypothesis involving the extension and dynamics of the last glaciation period in North America, and how this could have shaped the distribution and dispersal ofV. microcarpum. Finally, we provided important data regarding the polyploid origin ofV. oxycoccos.