bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compression-induced expression of glycolysis genes in CAFs correlates with EMT and angiogenesis gene expression in breast cancer
Authors: Kim Baek Gil , Sung Jin Sol , Jang Yeonsue , Cha Yoon Jin , Kang Suki , Han Hyun Ho , Lee Joo Hyun , Cho Nam Hoon 
Published: August 2019

AbstractTumor growth increases compressive stress within a tissue, which is associated with solid tumor progression. However, very little is known about how compressive stress contributes to tumor progression. Here, we show that compressive stress induces glycolysis in human breast cancer associated fibroblast (CAF) cells and thereby contributes to the expression of epithelial to mesenchymal (EMT)- and angiogenesis-related genes in breast cancer cells. Lactate production was increased in compressed CAF cells, in a manner dependent on the expression of metabolic genes ENO2, HK2, and PFKFB3. Conditioned medium from compressed CAFs promoted the proliferation of breast cancer cells and the expression of EMT and/or angiogenesis-related genes. In patient tissues with high compressive stress, the expression of compression-induced metabolic genes was significantly and positively correlated with EMT and/or angiogenesis-related gene expression and metastasis size. These findings illustrate a mechanotransduction pathway involving stromal glycolysis that may be relevant also for other solid tumours.
Mutant L-chain ferritins that cause neuroferritinopathy alter ferritin functionality and iron permeability
Authors: McNally Justin R , Mehlenbacher Matthew R , Luscieti Sara , Smith Gideon L , Reutovich Aliaksandra A , Maura Poli , Arosio Paolo , Bou-Abdallah Fadi 
Published: August 2019

Abstract
In mammals, the iron storage and detoxification protein ferritin is composed of two functionally and genetically distinct subunit types, H (heavy) and L (light). The two subunits co-assemble in various ratios, with a tissue specific distribution, to form shell-like protein structures of 24 subunits within which a mineralized iron core is stored. The H-subunits possess ferroxidase centers that catalyze the rapid oxidation of ferrous ions, whereas the L-subunit does not have such centers and is believed to play an important role in electron transfer reactions that occur during the uptake and release of iron. Pathogenic mutations on the L-chain lead to neuroferritinopathy, a neurodegenerative disease characterized by abnormal accumulation of ferritin inclusion bodies and iron in the central nervous system. Here, we have characterized the thermal stability, iron loading capacity, iron uptake, and iron release properties of ferritin heteropolymers carrying the three pathogenic L-ferritin mutants (L154fs, L167fs, and L148fs, which for simplicity we named Ln1, Ln2 and Ln3, respectively), and a non-pathogenic variant (L135P) bearing a single substitution on the 3-fold axes of L-subunits. The UV-Vis data show a similar iron loading capacity (ranging between 1800 to 2400 Fe(iii)/shell) for all ferritin samples examined in this study, with Ln2 holding the least amount of iron (i.e. 1800 Fe(iii)/shell). The three pathogenic L-ferritin mutants revealed higher rates of iron oxidation and iron release, suggesting that a few mutated L-chains on the heteropolymer have a significant effect on iron permeability through the ferritin shell. DSC thermograms showed a strong destabilization effect, the severity of which depends on the location of the frameshift mutations (i.e. wt heteropolymer ferritin ≅ homopolymer H-chain &gt; L135P &gt; Ln2 &gt; Ln1 &gt; Ln3). Variant L135P had only minor effects on the protein functionality and stability, suggesting that local melting of the 3-fold axes in this variant may not be responsible for neuroferritinopathy-like disorders. The data support the hypothesis that hereditary neuroferritinopathies are due to alterations of ferritin functionality and lower physical stability which correlate with the frameshifts introduced at the C-terminal sequence and explain the dominant transmission of the disorder.
Extracellular regulated kinase 5 mediates osteoporosis through modulating viability and apoptosis of osteoblasts in ovariectomized rats
Authors: Guo Tuan-Mao , Xing Yan-Li , Zhu Hai-Yun , Yang Lan , Liu Guo-Xiong , Qiao Xi-Min 
Published: August 2019

Abstract
Postmenopausal osteoporosis is a common condition characterized by the increase and activation of osteoclasts. The present study aimed to investigate the effects of extracellular signal-regulated kinase (ERK) 5 (ERK-5) on postmenopausal osteoporosis by regulating the biological behaviors of osteoblasts. Sprague–Dawley (SD) rats were ovariectomized to develop an osteoporosis model. A lentivirus packaging system was employed to generate lentiviruses capable of up- or down-regulating the expression of ERK-5 in ovariectomized rats. The femoral biomechanical properties, bone mineral density (BMD), contents of calcium (Ca), phosphorus (P) and alkaline phosphatase (ALP) and bone turnover markers in rats, as well as viability, cycle and apoptosis of osteoblasts and ALP activity in osteoblasts were measured in the ovariectomized rats so as to explore the functional significance of ERK-5 in postmenopausal osteoporosis. The femoral mechanical strength of ovariectomized rats was enhanced by overexpression of ERK-5. Meanwhile femoral BMD, and bone metabolism were increased, and bone turnover normalized in the ovariectomized rats when ERK-5 was overexpressed. Lentivirus-mediated ERK-5 overexpression in osteoblasts was observed to inhibit osteoblast apoptosis, and promote viability, accompanied with increased ALP activity. Taken together, ERK-5 could decelerate osteoblast apoptosis and improve postmenopausal osteoporosis by increasing osteoblast viability. Thus, our study provides further understanding on a promising therapeutic target for postmenopausal osteoporosis.
Synovium-derived stromal cell-induced osteoclastogenesis: a potential osteoarthritis trigger
Authors: Dicarlo Manuela , Teti Gabriella , Cerqueni Giorgia , Iezzi Iolanda , Gigante Antonio , Falconi Mirella , Mattioli-Belmonte Monica 
Published: August 2019

Abstract
Purpose: To shed light on the idea that mesenchymal stem/stromal cells (MSCs) recruited in synovium (SM) (i.e. Synovium-Derived Stromal Cells, SDSCs) could be involved in Osteoarthritis (OA) pathophysiology. Attention was also paid to a further stromal cell type with a peculiar ultrastructure called telocytes (TCs), whose role is far from clarified. Methods: In the present in vitro study, we compared SDSCs isolated from healthy and OA subjects in terms of phenotype, morphology and differentiation potential as well as in their capability to activate normal Peripheral Blood Mononuclear Cells (PBMCs). Histological, immunohistochemical and ultrastructural analyses were integrated by qRT-PCR and functional resorbing assays. Results: Our data demonstrated that both SDSC populations stimulated the formation of osteoclasts from PBMCs: the osteoclast-like cells generated by healthy-SDSCs via transwell co-cultures were inactive, while OA-derived SDSCs have a much greater effectiveness. Moreover, the presence of TCs was more evident in cultures obtained from OA subjects and suggests a possible involvement of these cells in OA. Conclusions: Osteoclastogenic differentiation capability of PBMCs from OA subjects, also induced by B synoviocytes has been already documented. Here we hypothesized that SDSCs, generally considered for their regenerative potential in cartilage lesions, have also a role in the onset/maintenance of OA. Clinical relevance: Our observations may represent an interesting opportunity for the development of a holistic approach for OA treatment, that considers the multifaceted capability of MSCs in relation to the environment.
Coupled autotransformer and magnetic‐control soft‐start method for super‐large‐capacity high‐voltage motors
Authors: Yuan Jiaxin , Wang Chuansheng , Yin Shan , Wei Liangliang , Muramatsu Kazuhiro , Chen Baichao 
Published: August 2019

The large current generated by a direct start of the super‐large‐capacity high‐voltage induction motor would have a huge impact on the power grid as well as the motor itself. Traditional soft starters have the shortcomings of discontinuous adjustment, voltage sags, sudden torque mutation, secondary current impact and high cost. To resolve this issue, the authors propose a novel coupled autotransformer and magnetic‐control (CATMC) soft‐start method. The structure of the new CATMC soft starter combines the functions of the autotransformer and magnetic control reactor via an innovative electric and magnetic circuit design. In this study, the authors analyse the magnetic circuit structure and working principles of the CATMC soft starter. Then, to validate its principle and performance, the authors conduct a simulation study using ANSYS software and design and test an 18 MW/10 kV CATMC soft starter prototype. The simulation results demonstrate that the CATMC soft starter effectively avoids secondary current impact and constrains the motor starting current to <2.5 times the rated current. The public connection point bus voltage also meets the voltage sag requirement of the IEEE standard and reduces the impact on the power grid.
Distribution grid response monitor
Authors: Gao Ziran , Mao Daijiafan , Wang Jiankang 
Published: August 2019

The fast‐growing penetration of plug‐in electric vehicles (PEV) will become a primary challenge to the reliability of the power distribution grid (below 69 kV). The impulsive charging characteristics of PEV could accelerate grid assets’ depreciation and deteriorate power quality by instantaneously drawing massive power. This study introduces a software prototype, distribution grid response monitor (DGROM), which allows for assessment of PEV's dynamic impact on the distribution power system and predicts the range of the charging impact on asset depreciation. Distinct from related studies, DGROM allows for customised inputs (e.g. driving habits, charging stations, and PEV types) in PEV's impact analysis and visualises the results. Furthermore, by deploying an optimistic mitigation algorithm, DGROM forecasts the upper and lower bound of the distribution system response under PEV penetration. The authors describe the mathematical model and analysis methods of DGROM and demonstrate its validity on the distribution networks located in Oakland, Ohio.
Elastic-Plastic Stresses in Shrink Fit with A Solid Shaft
Authors: Mouâa Amal , Laghzale Nor Eddine , Bouzid Abdel-Hakim 
Published: August 2019

Shrink fit joining a solid shaft and a cylinder requires an accurate estimate of the residual contact pressure to transmit high powers, as in the case of gas turbines. Such torques require deformations of materials beyond their elastic limits.
This paper presents an analytical model that analyses the stresses in a shrink fit assembly consisting of a solid shaft and a cylindrical hub operating in the elastic-plastic range. Assuming the hub to have a nonlinear work-hardening material behavior, the distribution of stresses as functions of the interference, and the effect of geometry on contact pressure and on interference are exhibited. To validate the analytic method, Finite Element Method was used.
Study of the mechanical behavior of light mortars produced by thermal treatment, with coal waste of Jerada mine (eastern Morocco)
Authors: Addou R. , Hannawi Salmo K. , Zenasni Z. , Agbodjan W. P. , Zenasni M. 
Published: August 2019

This paper investigates the mechanical properties of light mortars containing coal waste of Jerada mine, as a volume replacement for sand, with different percentages of substitution: 10%, 20% and 50%.
The results revealed a decrease in the mechanical properties of composite mortars, including uniaxial compression and flexural strength as well as rigidity modulus. However, the heat treatment improves their ductility, and delays the propagation of cracks.
Thus, the developed material is interesting for use in construction, serving as a basis for manufacturing prefabricated blocks treated at 600°C. These elements can be used for applications with large deformations, or with mechanical or acoustic vibrations.
Spezialisierte Hochschulambulanz mit Querschnittscharakter:
Die Poliklinik für Plastische und Ästhetische Chirurgie
Authors: Dastagir Khaled , Limbourg Anne , Tecklenburg Andreas , Vogt Peter M. 
Published: August 2019

Zusammenfassung
Hintergrund Hochschulambulanzen haben eine zentrale Position in der
Behandlung, Lehre und Erforschung spezieller und komplexer Erkrankungen inne.
Der tägliche Betrieb einer universitären Poliklinik für Plastische, Ästhetische,
Hand- und Wiederherstellungschirurgie weist ein weitgefächertes Diagnosespektrum
der zugewiesenen Patienten aus. Unsere Hypothese war, dass das Diagnosespektrum
über den fachspezifischen Charakter hinaus weitreichend interdisziplinär ist und
ein hohes Maß an differenzierter und erweiterter klinischer Kompetenz
vermittelt.
Methode Die epidemiologischen Daten und Diagnosen der Patienten, die sich
zwischen 2013–2014 in unserer Ambulanz vorstellten, wurden anhand des
Arztbriefs, der medizinischen Akte und der Leistungsverschlüsselung (ICD)
katalogisiert. Eine Unterteilung der Diagnosen erfolgte nach Zugehörigkeit zu
den medizinischen Fachdisziplinen.
Ergebnisse Von 2013 und 2014 wurden 9272 Patienten mit 821 verschiedene
ICDs behandelt. Eine operative Haupt- oder Nebendiagnose bestand in 57 % der
Fälle. In 36 % der Diagnosen lagen internistische Krankheitsbilder vor, während
7 % aus dem Bereich Dermatologie, Neurologie, Psychiatrie, Zahnmedizin und
Augenheilkunde stammten. Die Alterschirurgie umfasste 22 %, postonkologische
Folgen 7 %, postoperatives Komplikationsmanagement 6 %, einer Z. n.
konservativer Behandlung 3 % und angeborene Fehlbildungen 3,0 % der Diagnosen.
Bei 29 % der vorgestellten Patienten stellten wir eine plastisch-chirurgische
Behandlungsindikation.
Schlussfolgerung Unsere Analyse zeigt, dass in einer Hochschulambulanz für
Plastische und Ästhetische Chirurgie, ein Patientenkollektiv mit komplexen
Erkrankungen und einem breiten Spektrum an Haupt- und Nebendiagnosen behandelt
wird. Dieses Spektrum bildet bei Ärzten eine breite Kompetenz aus und ist
aufgrund der Interdisziplinarität und Vermittlung von differentialdiagnostischen
Algorithmen und Untersuchungstechniken für die allgemeine Ausbildung von
Studierenden besonders geeignet.
Nutzungsakzeptanz einer Online-Intervention zur Verbesserung depressiver Symptome bei Menschen mit Adipositas
Authors: Löbner Margrit , Stein Janine , Rechenberg Theresia , Förster Franziska , Dorow Marie , Keller Jana , Weidauer Enrico , Riedel-Heller Steffi G. 
Published: August 2019

Zusammenfassung
Ziel der Studie Untersuchung der Nutzungsakzeptanz und assoziierter Faktoren in Bezug auf den Einsatz einer ungeleiteten Online-Intervention bei Menschen mit Adipositas und komorbiden depressiven Symptomen.
Methodik Quantitative längsschnittliche Pilotuntersuchung zur Nutzungsakzeptanz (Baseline vor Zugang zur Online-Intervention; Follow-up nach 3 Monaten) bei N = 46 Probanden.
Ergebnisse Mittlere bis hohe Nutzungsakzeptanz konnte gegenüber der Online-Intervention gezeigt werden. Die Nutzungsaufnahmerate lag bei 76,1 %, die Rate der vollständigen Bearbeitung bei 22,9 %. Positive Assoziationen zur Nutzungsakzeptanz konnten u. a. im Hinblick auf Persönlichkeitsmerkmale und den Bezug einer Erwerbsminderungsrente gezeigt werden.
Schlussfolgerung Online-Interventionen für Menschen mit Adipositas und komorbiden depressiven Symptomen können einen ergänzenden Behandlungsbaustein darstellen. Assoziierte Faktoren von Nutzungsakzeptanz sollten bei der Implementierung von Online-Interventionen beachtetet werden.
Arbeitstätigkeit nach einer Rückenmarksläsion
– Daten aus dem Ambulatorium einer paraplegiologischen
Rehabilitationsklinik
Authors: Hug Kerstin , Hummel Beate , Rinaldo Carmen , Saleh Christian , Lochmann Holger , Hund-Georgiadis Margret 
Published: August 2019

Zusammenfassung
Ziel Anhand von ambulanten Routinedaten und eines ICF-basierten
Fragebogens wurden Einflussfaktoren auf die Arbeitstätigkeit nach
einer Rückenmarksläsion überprüft.
Methodik Die retrospektive Auswertung basierte auf einer Stichprobe
von 290 Patienten mit Querschnittlähmung, davon 43%
arbeitstätig. In bivariaten Analysen und multiplen
Regressionsmodellen wurden 15 mögliche Einflussfaktoren
evaluiert.
Ergebnisse Negative Zusammenhänge mit der
Arbeitstätigkeit zeigten sich für die Faktoren
„Alter bei Rückenmarksläsion“ (OR: 0,96,
95%-KI: 0,93–0,99, p<0,002) und „Probleme
beim Zurücklegen von Entfernungen“ (OR: 0,44,
95%-KI: 0,25–0,79, p<0,006). Ein ähnlicher
Trend bestand für „Schwierigkeiten beim
Einkaufen“.
Schlussfolgerungen Die Resultate weisen darauf hin, dass neben dem
Alter bei Rückenmarksläsion auch Aspekte der
Mobilität für die Arbeitstätigkeit von Personen mit
Querschnittlähmungen entscheidend sind. Interventionen zur
Verbesserung der Mobilität sollten daher in der
Langzeitrehabilitation einen hohen Stellenwert einnehmen.
Immune and Hormonal Response to High-intensity Exercise During Orienteering
Authors: Araujo Nayara Costa , Neto Anibal Monteiro Magalhães , Fujimori Mahmi , Bortolini Miguel Sordi , Justino Allisson Benatti , Honorio-França Adenilda Cristina , Luzía França Eduardo 
Published: August 2019

AbstractOrienteering is an endurance sport that combines physical and cognitive activity, during which the athlete must complete a course with several points distributed over unknown terrain in the shortest possible time. A number of studies have investigated the body’s physiological adaptations to the stress caused during competition, but not the immunological changes. To that end, the present study evaluated the immunological, physiological and pathological responses in athletes performing high-intensity physical exercise during an orienteering race. The 30 athletes tested belonged to the elite orienteering category and participated in the regional championship. Cortisol levels were determined before and after the competition to assess stress response, as were the cytokines IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ and IL-17 to evaluate the immune response. Cortisol levels increased after the competition, indicating a stress condition. IFN-γ, IL-6 and IL-10 levels also rose post competition. The results indicate that orienteers are exposed to high stress levels, and that this condition affects their immune and endocrine systems, triggering a predominantly anti-inflammatory response, likely an athlete’s mechanism of adaptation to the stress imposed by high-intensity physical exercise.
Minimal-activity PET/CT for efficacy control after SIRT (MAPECSI) – clinical implementation of a resource-saving, liver-focused protocol
Authors: Gühne Falk , Drescher Robert , Seifert Philipp , Freesmeyer Martin 
Published: August 2019

Abstract
Aim SIRT is an established treatment option for liver malignancies. Metabolic information can provide additional knowledge about tumoral characteristics and treatment response. FDG-PET/CT was shown to be advantageous for pre-/post-SIRT evaluation. However, whole-body PET/CT is an elaborate procedure. The aim of the study was to optimize clinical efficacy assessment after SIRT with a low-dose, low-cost protocol for focused diagnostic work-up.
Methods An abdomen-only minimal-activity FDG-PET/CT protocol (MA-PET) was established as an alternative for clinically indicated whole-body PET/CT scans. After administering 40 MBq of F-18-FDG one bed position was scanned for 15 minutes. Scans were acquired before (initial scan), one month after (interim scan) and three months after SIRT (follow-up scan). Metabolic tumor activity was evaluated and was compared to standard CT follow-up results.
Results 50 lobar SIRT procedures in 37 patients were analysed. HCC (28), hepatic metastases (15) and CCC (7) were treated. In 18 liver lobes initial MA-PET did not show hypermetabolic lesions, 32 liver lobes underwent interim and follow-up MA-PET. All 114 MA-PET were technically feasible. Mean radiation dose was 1.9 mSv. 64 % of HCC presented low metabolism at baseline, whereas metastases and CCC were all clearly PET-positive. Majority of radiated liver tumors showed at least partial metabolic response. PET/CT results diverged from follow-up CT in 63 % of cases.
Conclusion Minimal-Activity FDG-PET/CT of the liver is a feasible tool for efficacy assessment after SIRT with low financial and radiation burden. It provides additional information to morphologic imaging modalities, which can be helpful in response appraisal and treatment planning.
Developing Infographics to Facilitate HIV-Related Patient–Provider Communication in a Limited-Resource Setting
Authors: Stonbraker Samantha , Halpern Mina , Bakken Suzanne , Schnall Rebecca 
Published: August 2019

Background Productive patient–provider communication is a recognized component of high-quality health care that leads to better health outcomes. Well-designed infographics can facilitate effective communication, especially when culture, language, or literacy differences are present.
Objectives This study aimed to rigorously develop infographics to improve human immunodeficiency virus (HIV)-related patient–provider communication in a limited-resource setting. A secondary purpose was to establish through participant feedback that infographics convey intended meaning in this clinical and cultural context.
Methods We adapted a participatory design methodology, developed in a high-resource setting, for use in the Dominican Republic. Initially, content to include was established using a data-triangulation method. Then, infographics were iteratively generated and refined during five phases of design sessions with three stakeholder groups: (1) 25 persons living with HIV, (2) 8 health care providers, and (3) 5 domain experts. Suggestions for improvement were incorporated between design sessions and questions to confirm interpretability of infographics were included at the end of each session.
Results Each participant group focused on different aspects of infographic designs. Providers drew on past experiences with patients and offered clinically and contextually relevant recommendations of symbols and images to include. Domain experts focused on technical design considerations and interpretations of infographics. While it was difficult for patient participants to provide concrete suggestions, they provided feedback on the meaning of infographics and responded clearly to direct questions regarding possible changes. Fifteen final infographics were developed and all participant groups qualitatively confirmed that they displayed the intended content in a culturally appropriate and clinically meaningful way.
Conclusion Incorporating perspectives from various stakeholders led to the evolution of designs over time and generated design recommendations that will be useful to others creating infographics for use in similar populations. Next steps are to assess the feasibility of using infographics to improve clinical communication and patient outcomes.