bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of spider toxin in entomopathogenic fungus Lecanicillium muscarium and selection of the strain showing efficient secretion of the recombinant protein
Authors: Timofeev Sergey , Mitina Galina , Rogozhin Eugene , Dolgikh Viacheslav 
Published: August 2019

ABSTRACT
Beta/delta-agatoxin-1 of spider Agelena orientalis was expressed in entomopathogenic fungus Lecanicillium muscarium. To ensure secretion of the recombinant product by the fungus, the signal secretory peptide of the Metarhizium anisopliae Mcl1 protein was inserted into the sequence. For detection of the recombinant product and selection of transformants, the toxin sequence was also fused with eGFP at the C-terminus. The gene encoding the A. orientalis toxin with the Mcl1 protein signal peptide was commercially synthesized, amplified and cloned into the vector pBARGPE1 designed for heterologous expression under the control of the PgpdA promoter and the trpC terminator of Aspergillus nidulans. A double selection on selective medium and microscopic analysis of transformants allowed obtaining a mitotically stable recombinant strain of L. muscarium. The recognition of the Mcl1 derived signal peptide in the cells of transformants and effective secretion of the hybrid product was confirmed by immunoblotting.
The Longevity-Associated SH2B3 (LNK) Genetic Variant: Selected Aging Phenotypes in 379,758 Subjects
Authors: Kuo Chia-Ling , Joaquim Micaella , Kuchel George A , Ferrucci Luigi , Harries Lorna W , Pilling Luke C , Melzer David 
Published: August 2019

Abstract
Human SH2B3 is involved in growth factor and inflammation signaling. A SH2B3 missense variant (rs3184504) is associated with cardiovascular diseases plus breast, colorectal, and lung cancers, with highly correlated variants across the ATXN2/SH2B3/BRAP locus linked to parental age at death, suggesting a geroscience common mechanism of aging and disease. To better understand the SH2B3-related aging pathway and its potential as an intervention target, we undertook a phenotype-wide association study (PheWAS) of 52 aging traits. Data were obtained from 379,758 European-descent UK Biobank participants, aged 40–70 at baseline: 27% of participants were CC homozygotes and 23% TT at rs3184504. Parental extreme longevity (mothers aged ≥98 years, fathers aged ≥96 years) was more common in CC versus TT (odds ratio [OR] = 1.18, 95% confidence interval [CI]: 1.07 to 1.29) with an additive per allele effect. The C allele associated with better cognitive function and white blood cell counts were more likely to be normal. The C allele reduced risks of coronary heart disease (OR = 0.95, 95% CI: 0.93 to 0.96) but was also associated with a modestly higher cancer rate (OR = 1.03, 95% CI: 1.02 to 1.04), suggesting a trade-off across aging outcomes and limiting its potential as an anti-aging target.
On the S-instability and degeneracy of discrete deep learning models
Authors: Kaplan Andee , Nordman Daniel J , Vardeman Stephen B 
Published: August 2019

AbstractA probability model exhibits instability if small changes in a data outcome result in large and, often unanticipated, changes in probability. This instability is a property of the probability model, given by a distributional form and a given configuration of parameters. For correlated data structures found in several application areas, there is increasing interest in identifying such sensitivity in model probability structure. We consider the problem of quantifying instability for general probability models defined on sequences of observations, where each sequence of length $N$ has a finite number of possible values that can be taken at each point. A sequence of probability models, indexed by $N$, and an associated parameter sequence result to accommodate data of expanding dimension. Model instability is formally shown to occur when a certain log probability ratio under such models grows faster than $N$. In this case, a one component change in the data sequence can shift probability by orders of magnitude. Also, as instability becomes more extreme, the resulting probability models are shown to tend to degeneracy, placing all their probability on potentially small portions of the sample space. These results on instability apply to large classes of models commonly used in random graphs, network analysis and machine learning contexts.
Legitimacy and the Cognitive Sources of International Institutional Change: The Case of Regional Parliamentarization
Authors: Lenz Tobias , Burilkov Alexandr , Viola Lora Anne 
Published: August 2019

AbstractHow and under what conditions does legitimacy affect processes of international institutional change? This article specifies and evaluates three causal mechanisms by which variation in legitimacy induces institutional change in international organizations (IOs) and argues that an important, yet hitherto neglected, source of legitimacy-based change is cognitive in nature. Using survival analysis, we evaluate these mechanisms with a novel dataset on the establishment of parliamentary institutions in thirty-six regional organizations between 1950 and 2010. We find that the empowerment of supranational secretariats, engagement with the European Union, and parliamentarization in an organization's neighborhood increase the likelihood of regional parliamentarization. This suggests that legitimacy judgments that draw on cognitive referents provide an important source of international institutional change. We illustrate the underlying cognitive emulation mechanism with a case study of parliamentarization in the Association of Southeast Asian Nations.
Directional presplit blasting in an innovative no-pillar mining approach
Authors: Hu Jinzhu , Zhang Xingyu , Gao Yubing , Ma Zimin , Xu Xinzeng , Zhang Xiuping 
Published: August 2019

Abstract
Directional presplit blasting (DPB) technology for an innovative no-pillar mining approach is introduced. First, a mechanical model is established to analyze crack initiation and coalescence using the new technology. The explosion pressure formula containing tress concentration factor explains the relationship between the quantity of explosives and the crack initiation and coalescence. Subsequently, a dynamic finite element simulation method is used to study the stress and damage evolution process of the blasthole wall in ordinary explosion and DPB. The simulation results indicate that the stress concentration factor is approximately 4c10 for the same quantity of explosives. Finally, a field test was conducted in the Fucheng coal mine of China. Based on the field test, the concept of cracking ability is proposed, the relationship between the quantity of explosives and cracking ability is determined, and the quantity of explosives is divided into four stages that are compatible with the roof rock strength. The results can provide some clinical guidance for application of DPB in other projects of the gob-side entry retaining by roof cutting.
Cementless bipolar hemiarthroplasty in a patient with PMF: a case report
Authors: Alturki Abdullah A , Alshammari Nayf A , Alsebayel Firas M , Alhandi Ali A 
Published: August 2019

AbstractMyelofibrosis is a myeloproliferative disease that falls under a group of bone marrow malignancies known as myeloproliferative neoplasms. It manifests with splenomegaly, anemia, leukocytosis and, less commonly, bone pain. Ruxolitinib, Janus kinase inhibitor, has been shown to increase survival, to improve symptoms and has the potential to decrease osteosclerotic changes. Herein, we present a case of primary myelofibrosis (PMF) in a 60-year-old female who presented with 8-month history of progressive left hip pain and later was diagnosed with pathological neck of femur fracture that was treated with cementless hemiarthroplasty. In conclusion, the use of cementless implants in hip arthroplasty in the presence of PMF has shown to be an effective and safe choice.
Properties of the post-inspiral common envelope ejecta – I. Dynamical and thermal evolution
Authors: Iaconi Roberto , Maeda Keiichi , De Marco Orsola , Nozawa Takaya , Reichardt Thomas 
Published: August 2019

ABSTRACT
We investigate the common envelope binary interaction, that leads to the formation of compact binaries, such as the progenitors of Type Ia supernovae or of mergers that emit detectable gravitational waves. In this work, we diverge from the classic numerical approach that models the dynamic inspiral. We focus instead on the asymptotic behaviour of the common envelope expansion after the dynamic inspiral terminates. We use the SPH code phantom to simulate one of the set-ups from Passy et al., with a 0.88 M⊙, 83 R⊙ RGB primary and a 0.6 M⊙ companion, then we follow the ejecta expansion for 50 yr. Additionally, we utilize a tabulated equation of state including the envelope recombination energy in the simulation (Reichardt et al.), achieving a full unbinding. We show that, as time passes, the envelope’s radial velocities dominate over the tangential ones, hence allowing us to apply an homologous expansion kinematic model to the ejecta. The external layers of the envelope become homologous as soon as they are ejected, but it takes 5000 d (14 yr) for the bulk of the unbound gas to achieve the homologously expanding regime. We observe that the complex distribution generated by the dynamic inspiral evolves into a more ordered, shell-like shaped one in the asymptotic regime. We show that the thermodynamics of the expanding envelope are in very good agreement with those expected for an adiabatically expanding sphere under the homologous condition and give a prediction for the location and temperature of the photosphere assuming dust to be the main source of opacity. This technique ploughs the way to determining the long-term light behaviour of common envelope transients.
Elemental abundances in the centre of the Galactic nuclear disc
Authors: Kovtyukh V V , Andrievsky S M , Martin R P , Korotin S A , Lepine J R D , Maciel W J , Keir L E , Panko E A 
Published: August 2019

ABSTRACT
We have made the first attempt to derive the chemical properties of the Galactic disc at its very central part using high-resolution infrared spectroscopic observations of four classical Cepheids. These stars are located at Galactocentric distances smaller than 1 kpc. All investigated stars show near-to-solar elemental abundances. By combining these new data with our previous studies, our results suggest that the radial distribution of iron abundance on a logarithmic scale gradually increases from the outskirts of the Galactic disc to Galactocentric distances of about 2–4 kpc. There, it reaches a maximal value of about +0.4 dex, and then declines sharply to about the solar value at the Galactic Centre.
Queer Music Theory
Authors: Lee Gavin 
Published: August 2019

AbstractQueer musical phenomenology refers to the practice of disorientation away from established music theories, including one’s own. In Lewin’s “Phenomenology” article, queering can be understood as his intentional, self-critical, conceptual disorientations—first departing from Schenkerian theory, and then moving toward and finally away from the perception-model. Through a close reading of Lewin in combination with Sara Ahmed’s Queer Phenomenology, which offers a theory of embodied lives marginalized by pathways of normativity, I examine the generalizable application of theories such as queer phenomenology to another domain beyond gender and sexual embodiment: music theory at large. Lewin’s practice models a form of music theory that I regard as phenomenologically queer.
Emerging Therapeutic Targets in Chordomas: A Review of the Literature in the Genomic Era
Authors: Gill Corey M , Fowkes Mary , Shrivastava Raj K 
Published: August 2019

Abstract
Chordomas are rare primary malignant tumors of the bones that occur along the skull base, spine, and sacrum. Long-term survival and neurological outcome continue to be challenging with continued low percentages of long-term survival. Recent studies have used genome, exome, transcriptome, and proteome sequencing to assess the mutational profile of chordomas. Most notably, Brachyury, or T-protein, has been shown to be an early mutational event in chordoma evolution. Clinically actionable mutations, including in the PI3K pathway, were identified. Preliminary evidence suggests that there may be mutational differences associated with primary tumor location. In this study, we review the therapeutic landscape of chordomas and discuss emerging targets in the genomic era.
Prediction of recurrent glioblastoma after laser interstitial thermal therapy: The role of diffusion imaging
Authors: Mahammedi Abdelkader , Bachir Suha , Escott Edward J , Barnett Gene H , Mohammadi Alireza M , Larvie Mykol 
Published: August 2019

Abstract

Background
Evaluate the utility of diffusion-weighted imaging (DWI) for the assessment of local recurrence of glioblastoma (GBM) on imaging performed 24 h following MRI-guided laser interstitial thermal therapy (LITT). We hypothesize that microscopic peritumoral infiltration correlates with early subtle variations on DWI images and apparent diffusion coefficient (ADC) maps.

Methods
Of 64 patients with GBM treated with LITT, 39 had MRI scans within 24 h after undergoing LITT. Patterns on DWI images and ADC maps 24 h following LITT were correlated with areas of future GBM recurrence identified through coregistration of subsequent MRI examinations. In the areas of suspected recurrence within the periphery of post-LITT lesions, signal intensity values on ADC maps were recorded and compared with the remaining peritumoral ring.

Results
Thirty-nine patients with GBM met the inclusion criteria. For predicting recurrent GBM, areas of decreased DWI signal and increased signal on ADC maps within the expected peritumoral ring of restricted diffusion identified 24 h following LITT showed 86.1% sensitivity, 75.2% specificity, and high correlation (r = 0.53) with future areas of GBM recurrence (P &lt; .01). Areas of future recurrence demonstrated a 37% increase in the ADC value (P &lt; .001), compared with findings in the surrounding treated peritumoral region. A significantly greater area under the receiver operating characteristics curve was determined for ADC values (P &lt; .01).

Conclusions
DWI obtained 24 h following LITT can help predict the location of GBM recurrence months before the development of abnormal enhancement. This may alter future treatment planning, perhaps suggesting areas that may be targeted for additional therapy.
Expansion of effector and memory T cells is associated with increased survival in recurrent glioblastomas treated with dendritic cell immunotherapy
Authors: Eoli Marica , Corbetta Cristina , Anghileri Elena , Di Ianni Natalia , Milani Micaela , Cuccarini Valeria , Musio Silvia , Paterra Rosina , Frigerio Simona , Nava Sara , Lisini Daniela , Pessina Sara , Maddaloni Luisa , Lombardi Raffaella , Tardini Maria , Ferroli Paolo , DiMeco Francesco , Bruzzone Maria Grazia , Antozzi Carlo , Pollo Bianca , Finocchiaro Gaetano , Pellegatta Serena 
Published: August 2019

Abstract

Background
The efficacy of dendritic cell (DC) immunotherapy as a single therapeutic modality for the treatment of glioblastoma (GBM) patients remains limited. In this study, we evaluated in patients with GBM recurrence the immune-mediated effects of DC loaded with autologous tumor lysate combined with temozolomide (TMZ) or tetanus toxoid (TT).

Methods
In the phase I-II clinical study DENDR2, 12 patients were treated with 5 DC vaccinations combined with dose-dense TMZ. Subsequently, in eight patients, here defined as Variant (V)-DENDR2, the vaccine site was preconditioned with TT 24 hours before DC vaccination and TMZ was avoided. As a survival endpoint for these studies, we considered overall survival 9 months (OS9) after second surgery. Patients were analyzed for the generation of effector, memory, and T helper immune response.

Results
Four of 12 DENDR2 patients reached OS9, but all failed to show an immunological response. Five of eight V-DENDR2 patients (62%) reached OS9, and one patient is still alive (OS &gt;30 months). A robust CD8+ T-cell activation and memory T-cell formation were observed in V-DENDR2 OS&gt;9. Only in these patients, the vaccine-specific CD4+ T-cell activation (CD38+/HLA-DR+) was paralleled by an increase in TT-induced CD4+/CD38low/CD127high memory T cells. Only V-DENDR2 patients showed the formation of a nodule at the DC injection site infiltrated by CCL3-expressing CD4+ T cells.

Conclusions
TT preconditioning of the vaccine site and lack of TMZ could contribute to the efficacy of DC immunotherapy by inducing an effector response, memory, and helper T-cell generation.
Controlling the Uncontrollable: Perceptions of Balance in People With Parkinson Disease
Authors: Johansson Hanna , Franzén Erika , Skavberg Roaldsen Kirsti , Hagströmer Maria , Leavy Breiffni 
Published: August 2019

Abstract

Background
Exercise improves balance in Parkinson disease (PD), yet the majority of people with the diagnosis are physically inactive. Insights gained from understanding how people with PD (PwPD) make sense of their symptoms and their ability to control them may inform the communication strategies and motivational approaches adopted by physical therapists. To our knowledge, no previous study has qualitatively explored how PwPD perceive the concept of balance and the beliefs they hold concerning their ability to affect balance.

Objective
This study aimed to explore the meaning of balance for PwPD and the beliefs they hold regarding their ability to influence their balance in everyday life.

Design
The design was a qualitative study with an inductive approach.

Methods
In-depth interviews were conducted with 18 participants with PD (age range 46–83 years, Hoehn and Yahr range 1–4), and transcripts were analyzed using qualitative content analysis.

Results
Five main themes emerged from the analysis: remaining in control over the body, adapting behavior to deal with uncertainty, directing focus to stay 1 step ahead, resilience as a defense, and exercise beliefs and reservations. Interpretation of the underlying patterns in the main themes yielded the overarching theme of focus and determination to regain control over shifting balance.

Conclusions
The concept of balance was perceived as both bodily equilibrium and mind-body interplay and was described in the context of remaining in control over one’s body and everyday life. Cognitive resources were utilized to direct focus and attention during balance-challenging situations in a process involving internal dialogue. Even participants who did not express beliefs in their ability to affect balance through exercise used psychological resilience to counter the challenges of impaired balance.
Solving the jigsaw of conflict-related environmental damage: Utilizing open-source analysis to improve research into environmental health risks
Authors: Zwijnenburg Wim , Hochhauser David , Dewachi Omar , Sullivan Richard , Nguyen Vinh-Kim 
Published: August 2019

Abstract
Investigation of the environmental impacts of armed conflict has been made easier in recent years with the development of new and improved methods for documenting and monitoring environmental damage and pollution. For decades, research into conflict-linked environmental damage and its links to human health have been overlooked and research underfunded, hindering a complete humanitarian response and effective post-conflict reconstruction. Recent developments in the field of open-source investigation have shown promising results due to the increased use of mobile phones, access to the internet and freely available methods for remote observation by satellite. Utilizing and analysing these sources of data can help us to understand how conflicts are associated with environmental damage, pollution and their negative impacts upon public health. Further research and development in this field will help to inform more effective humanitarian responses, mitigate risks to health and identify priorities for post-conflict reconstruction programs. Data-driven open-source research can also strengthen international discussions on state accountability for military activities and build a case for the responsibility of warring parties to protect the environment as well as the people who depend on it.
Treatment guidelines in psoriatic arthritis
Authors: Ogdie Alexis , Coates Laura C , Gladman Dafna D 
Published: August 2019

AbstractPsoriatic arthritis (PsA) is a complex inflammatory musculoskeletal and skin disease. The treatment of PsA has changed substantially over the past 10 years. Clinical practice guidelines are developed to help busy clinicians rapidly integrate evolving knowledge of therapeutic management into practice. In this review, we compare PsA treatment recommendations or guidelines developed by one national organization [ACR and National Psoriasis Foundation (NPF) in 2018], one regional organization (EULAR in 2015), and one international organization (the Group for Research and Assessment of Psoriasis and Psoriatic Arthritis in 2015). We examine the development of guidelines in PsA more broadly and examine similarities and differences in the three sets of recommendations.
Trends of venous thromboembolism risk before and after diagnosis of gout: a general population-based study
Authors: Li Lingyi , McCormick Natalie , Sayre Eric C , Esdaile John M , Lacaille Diane , Xie Hui , Choi Hyon K , Aviña-Zubieta J Antonio 
Published: August 2019

AbstractObjectiveTo estimate the overall risk and the temporal trend of venous thromboembolism (VTE), deep vein thrombosis (DVT), and pulmonary embolism (PE) before and after gout diagnosis in an incident gout cohort compared with the general population.MethodsWe conducted a matched cohort study using a province-wide population-based administrative health database in Canada. We calculated incidence rates (IRs) and multivariable adjusted hazard ratios (HRs) for the risk of VTE, DVT and PE before and after gout diagnosis.ResultsAmong 130 708 incident individuals with gout (64% male, mean age 59 years), 2071 developed VTE, 1377 developed DVT and 1012 developed PE. IRs per 1000 person-years for gout were 2.63, 1.74 and 1.28 compared with 2.03, 1.28 and 1.06 for non-gout, respectively. The fully adjusted HRs (95% CI) for VTE, DVT and PE were 1.22 (1.13, 1.32), 1.28 (1.17, 1.41) and 1.16 (1.05, 1.29). For the pre-gout period, the fully adjusted HRs (95% CI) were 1.51 (1.38, 1.64), 1.55 (1.40, 1.72) and 1.47 (1.31, 1.66) for VTE, DVT and PE. During the third, second and first years preceding gout, the fully adjusted HRs for VTE were 1.44, 1.56 and 1.62. During the first, second, third, fourth and fifth years after gout, the fully adjusted HRs were 1.63, 1.29, 1.33, 1.28 and 1.22. Similar trends were also seen for DVT and PE.ConclusionIncreased risks of VTE, DVT and PE were found both before and after gout diagnosis. The risk increased gradually before gout, peaking in the year prior to diagnosis, and then progressively declined. Gout-associated inflammation may contribute to venous thrombosis risk.