bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cancer-associated fibroblast subtypes modulate the tumor-immune microenvironment and are associated with skin cancer malignancy
Authors: Forsthuber Agnes , Aschenbrenner Bertram , Korosec Ana , Jacob Tina , Annusver Karl , Krajic Natalia , Kholodniuk Daria , Frech Sophie , Zhu Shaohua , Purkhauser Kim , Lipp Katharina , Werner Franziska , Nguyen Vy , Griss Johannes , Bauer Wolfgang , Soler Cardona Ana , Weber Benedikt , Weninger Wolfgang , Gesslbauer Bernhard , Staud Clement , Nedomansky Jakob , Radtke Christine , Wagner Stephan N. , Petzelbauer Peter , Kasper Maria , Lichtenberger Beate M. 
Published: November 2024

AbstractCancer-associated fibroblasts (CAFs) play a key role in cancer progression and treatment outcome. This study dissects the intra-tumoral diversity of CAFs in basal cell carcinoma, squamous cell carcinoma, and melanoma using molecular and spatial single-cell analysis. We identify three distinct CAF subtypes: myofibroblast-like RGS5+ CAFs, matrix CAFs (mCAFs), and immunomodulatory CAFs (iCAFs). Large-cohort tissue analysis reveals significant shifts in CAF subtype patterns with increasing malignancy. Two CAF subtypes exhibit immunomodulatory properties via different mechanisms. mCAFs sythesize extracellular matrix and may restrict T cell invasion in low-grade tumors via ensheathing tumor nests, while iCAFs are enriched in late-stage tumors, and express high levels of cytokines and chemokines to aid immune cell recruitment and activation. This is supported by the induction of an iCAF-like phenotype with immunomodulatory functions in primary healthy fibroblasts exposed to skin cancer cell secretomes. Thus, targeting CAF variants holds promise to enhance immunotherapy efficacy in skin cancers.
Novel tools to quantify total, phospho-Ser129 and aggregated alpha-synuclein in the mouse brain
Authors: Trist Benjamin Guy , Wright Courtney Jade , Rangel Alejandra , Cottle Louise , Prasad Asheeta , Jensen Nanna Møller , Gram Hjalte , Dzamko Nicolas , Jensen Poul Henning , Kirik Deniz 
Published: November 2024

AbstractAssays for quantifying aggregated and phosphorylated (S129) human α-synuclein protein are widely used to evaluate pathological burden in patients suffering from synucleinopathy disorders. Many of these assays, however, do not cross-react with mouse α-synuclein or exhibit poor sensitivity for this target, which is problematic considering the preponderance of mouse models at the forefront of pre-clinical α-synuclein research. In this project, we addressed this unmet need by reformulating two existing AlphaLISA® SureFire® Ultra™ total and pS129 α-synuclein assay kits to yield robust and ultrasensitive (LLoQ ≤ 0.5 pg/mL) quantification of mouse and human wild-type and pS129 α-synuclein protein. We then employed these assays, together with the BioLegend α-synuclein aggregate ELISA, to assess α-synuclein S129 phosphorylation and aggregation in different mouse brain tissue preparations. Overall, we highlight the compatibility of these new immunoassays with rodent models and demonstrate their potential to advance knowledge surrounding α-synuclein phosphorylation and aggregation in synucleinopathies.
Frequent rainfall-induced new particle formation within the canopy in the Amazon rainforest
Authors: Machado Luiz A. T. , Unfer Gabriela R. , Brill Sebastian , Hildmann Stefanie , Pöhlker Christopher , Cheng Yafang , Williams Jonathan , Hartwig Harder , Andreae Meinrat O. , Artaxo Paulo , Curtius Joachim , Franco Marco A. , Cecchini Micael A. , Edtbauer Achim , Hoffmann Thorsten , Holanda Bruna , Khadir Théodore , Krejci Radovan , Kremper Leslie A. , Liu Yunfan , Meller Bruno B. , Pöhlker Mira L. , Quesada Carlos A. , Ringsdorf Akima , Riipinen Ilona , Trumbore Susan , Wolff Stefan , Lelieveld Jos , Pöschl Ulrich 
Published: November 2024

AbstractAtmospheric aerosol particles are essential for forming clouds and precipitation, thereby influencing Earth’s energy budget, water cycle and climate on regional and global scales. However, the origin of aerosol particles over the Amazon rainforest during the wet season is poorly understood. Earlier studies showed new particle formation in the outflow of deep convective clouds and suggested a downward flux of aerosol particles during precipitation events. Here we use comprehensive aerosol, trace gas and meteorological data from the Amazon Tall Tower Observatory to show that rainfall regularly induces bursts of nanoparticles in the nucleation size range. This can be attributed to rain-related scavenging of larger particles and a corresponding reduction of the condensation sink, along with an ozone injection into the forest canopy, which could increase the oxidation of biogenic volatile organic compounds, especially terpenes, and enhance new particle formation. During and after rainfall, the nucleation particle concentrations directly above the canopy are greater than those higher up. This gradient persists throughout the wet season for the nucleation size range, indicating continuous particle formation within the canopy, a net upward flux of newly formed particles and a paradigm shift in understanding aerosol–cloud–precipitation interactions in the Amazon. Particle bursts provide a plausible explanation for the formation of cloud condensation nuclei, leading to the local formation of green-ocean clouds and precipitation. Our findings suggest that an interplay of a rain-related reduction in the condensation sink, primary emissions of gases, mainly terpenes, and particles from the forest canopy, and convective cloud processing determines the population of cloud condensation nuclei in pristine rainforest air.
Umbrella review of meta-analyses on the risk factors, protective factors, consequences and interventions of cyberbullying victimization
Authors: Kasturiratna K. T. A. Sandeeshwara , Hartanto Andree , Chen Crystal H. Y. , Tong Eddie M. W. , Majeed Nadyanna M. 
Published: November 2024

Abstract
The increasing prevalence of cyberbullying victimization has become a commonplace issue globally. Although research has explored various predictors and consequences of cyberbullying victimization, most focus on a narrow range of variables or contexts, highlighting the need to comprehensively review and synthesize the wealth of empirical findings. We conducted a systematic review of meta-analyses on cyberbullying victimization, incorporating 56 meta-analyses and 296 effect sizes (sample size range 421–1,136,080, sample size median 53,183; searched via EBSCOhost ERIC, EBSCOhost PsycInfo, PubMed, Scopus, Web of Science, 13 cyberbullying-related journals, Google Scholar and ProQuest Dissertations and Theses) to address the following critical questions: (1) What are the crucial sociodemographic and psychological profiles of cyberbullying victims? (2) What critical contextual and environmental factors are associated with cyberbullying victimization? (3) What are the key psychological and behavioural consequences of cyberbullying victimization? (4) How effective are existing interventions in mitigating impacts of cyberbullying? Included meta-analyses had to focus on cyberbullying victimization and report at least one predictor or consequence. A quality assessment was conducted using the Joanna Briggs Institute Critical Appraisal Instrument for Systematic Reviews and Research Syntheses. Findings suggest that females, school-aged populations, traditional bullying victims and frequent internet users were more likely to be cyberbullied. Unregulated school environments and unsupportive parental relationships were also associated with increased cyberbullying victimization. Cyberbullying victimization was consistently associated with negative psychological outcomes, lower school performance and maladaptive coping behaviours. More importantly, the current review found that cyberbullying intervention programmes show promising results. The current review underscores the importance of devoting adequate resources to mitigating cyberbullying victimization.
CartoMark: a benchmark dataset for map pattern recognition and map content retrieval with machine intelligence
Authors: Zhou Xiran , Wen Yi , Shao Zhenfeng , Li Wenwen , Li Kaiyuan , Li Honghao , Xie Xiao , Yan Zhigang 
Published: November 2024

AbstractMaps are fundamental medium to visualize and represent the real word in a simple and philosophical way. The emergence of the big data tide has made a proportion of maps generated from multiple sources, significantly enriching the dimensions and perspectives for understanding the characteristics of the real world. However, a majority of these map datasets remain undiscovered, unacquired and ineffectively used, which arises from the lack of numerous well-labelled benchmark datasets, which are of significance to implement the deep learning techniques into identifying complicated map content. To address this issue, we develop a large-scale benchmark dataset involving well-labelled datasets to employ the state-of-the-art machine intelligence technologies for map text annotation recognition, map scene classification, map super-resolution reconstruction, and map style transferring. Furthermore, these well-labelled datasets would facilitate map feature detection, map pattern recognition and map content retrieval. We hope our efforts would provide well-labelled data resources for advancing the ability to recognize and discover valuable map content.
Circular dichroism in multiphoton ionization of resonantly excited helium ions near channel closing
Authors: Wagner René , Ilchen Markus , Douguet Nicolas , Schmidt Philipp , Wieland Niclas , Callegari Carlo , Delk Zachary , Demidovich Alexander , De Ninno Giovanni , Di Fraia Michele , Hofbrucker Jiri , Manfredda Michele , Music Valerija , Plekan Oksana , Prince Kevin C. , Rivas Daniel E. , Zangrando Marco , Grum-Grzhimailo Alexei N. , Bartschat Klaus , Meyer Michael 
Published: November 2024

AbstractThe circular dichroism (CD) of photoelectrons generated by near-infrared (NIR) laser pulses using multiphoton ionization of excited He+ ions in the 3p(m= +1) state is investigated. The ions were prepared by circularly polarized extreme ultraviolet (XUV) pulses. For circularly polarized NIR pulses co- and counter-rotating relative to the polarization of the XUV pulse, a complex variation of the CD is observed as a result of intensity- and polarization-dependent Freeman resonances, with and without additional dichroic AC-Stark shifts. The experimental results are compared with numerical solutions of the time-dependent Schrödinger equation to identify and interpret the pronounced variation of the experimentally observed CD.
Visualizing the human olfactory projection and ancillary structures in a 3D reconstruction
Authors: Low Victoria F. , Lin Chinchien , Su Shan , Osanlouy Mahyar , Khan Mona , Safaei Soroush , Maso Talou Gonzalo , Curtis Maurice A. , Mombaerts Peter 
Published: November 2024

AbstractVisualizing in 3D the histological microanatomy of the human olfactory projection from the olfactory mucosa in the nasal cavity to the olfactory bulbs in the cranial cavity necessitates a workflow for handling a great many sections. Here, we assembled a 3D reconstruction of a 7.45 cm3en-bloc specimen extracted from an embalmed human cadaver. A series of 10 µm coronal sections was stained with quadruple fluorescence histology and scanned in four channels. A trained anatomist manually segmented six structures of interest in a subset of the sections to generate the ground truth. Six convolutional neural networks were then trained for automatic segmentation of these structures in 1234 sections. A high-performance computing solution was engineered to register the sections based on the fluorescence signal and segmented structures. The resulting 3D visualization offers several novel didactic opportunities of interactive exploration and virtual manipulation. By extrapolating manual counts of OSNs in a subset of sections to the calculated volume of the envelope of the entire olfactory epithelium, we computed a total of ~2.7 million OSNs in the specimen. Such empirically derived information helps assess the extent to which the organizational principles of the human olfactory projection may differ from those in mice.
Knowledge maps as a complementary tool to learn and teach surgical anatomy in virtual reality: A case study in dental implantology
Authors: Lúcio Inês M. , de Faria Bernardo G. , Raidou Renata G. , Proença Luís , Zagalo Carlos , Mendes José João , Rodrigues Pedro , Lopes Daniel Simões 
Published: November 2024

AbstractA thorough understanding of surgical anatomy is essential for preparing and training medical students to become competent and skilled surgeons. While Virtual Reality (VR) has shown to be a suitable interaction paradigm for surgical training, traditional anatomical VR models often rely on simple labels and arrows pointing to relevant landmarks. Yet, studies have indicated that such visual settings could benefit from knowledge maps as such representations explicitly illustrate the conceptual connections between anatomical landmarks. In this article, a VR educational tool is presented designed to explore the potential of knowledge maps as a complementary visual encoding for labeled 3D anatomy models. Focusing on surgical anatomy for implantology, it was investigated whether integrating knowledge maps within a VR environment could improve students' understanding and retention of complex anatomical relationships. The study involved 30 master's students in dentistry and 3 anatomy teachers, who used the tool and were subsequently assessed through surgical anatomy quizzes (measuring both completion times and scores) and subjective feedback (assessing user satisfaction, preferences, system usability, and task workload). The results showed that using knowledge maps in an immersive environment facilitates learning and teaching surgical anatomy applied to implantology, serving as a complementary tool to conventional VR educational methods.
Nano-Enhanced Fertilizers for Energy-Efficient Rice Farming: A Comparative Study
Authors: Kumar P.S. Satheesh , Rao Vittel , Kavitha N. , Balamurugan C. , Indira K.P. , Banga V.K. 
Published: November 2024

The objective of this study is to determine the differences in Oryza sativa (rice) growth, as cultivated on conventional pig manure relative to that on nano-enhanced poultry manure mixed with sesbania. Improving nutrient delivery and achieving better chemical reactions in the soil resulted in increased plant growth and sustainability when nanomaterials were added to poultry manure. The experimental field area covered 1000 square feet, divided into two groups: Group 1 is working with nano-enhanced poultry manure together with Sesbania, while Group 2 is using only pig dung. A total of 40 samples consisted of 20 samples from each group. Using G- power, the sample size of 80% power and 95% confidence interval was established, with statistical analysis performed in SPSS software. With 105– 150 days, the average plant height of nano-enhanced poultry manures along with Sesbania reached 87 cm, whereas the pig manure had just 70 cm, stated the results. The variation showed an p-value of 0.001 (p < 0.05), indicating it was statistically significant.
The improved performance of the manure is attributed to its greater chemical interactions and efficient nutrient absorption thanks to nanotechnology. This work provides important information on energy-efficient, high-yield farming techniques by bringing attention to the potential benefits of merging nanotechnology with organic fertilizers for sustainable rice cultivation. Results indicate that combining organic inputs with innovative technologies may greatly improve the quality of soil and nutrient supply, benefiting energy-efficient agriculture and sustainable manufacturing.
Traumatischer vollständiger Verlust des Kniestreckapparats und dessen Rekonstruktion mittels eines Fresh Frozen Allografts mit anheftender Patella- und Quadrizepssehne
Authors: Weber Johannes , Kerschbaum Maximilian , Klein Silvan , Hitzenbichler Florian , Rupp Markus , Alt Volker 
Published: November 2024

ZusammenfassungDer vorliegende Case Report handelt von einer 18-jährigen Patientin, die infolge eines Motorradunfalls mit schwerem ventralen Weichteilschaden über dem linken Knie und anschließender Infektion einen kompletten Verlust des Streckapparats (Patella, Quadrizeps- und Patellarsehne) des linken Kniegelenks erlitt. Nach Infektsanierung und erfolgreicher Einheilung eines freien Latissimus-dorsi-Lappens erfolgte die Rekonstruktion des Streckapparats mittels eines Fresh Frozen Streckapparat-Allografts mit Patella, Quadrizeps- und Patellarsehne mit integriertem knöchernem Tuberositas-tibia-Fragment. Nach einer Beobachtungszeit von 24 Monaten zeigt sich eine infektfreie Einheilung des Transplantates bei einer aktiven Beweglichkeit von Extension/Flexion von 0–0–90° und schmerzfreiem, nahezu normalem Gangbild.
Differences in 5-km running pace between female and male
triathletes
Authors: dos Anjos Vinicius Ribeiro , Vivan Lavínia , Engelke Paulo , de Lira Claudio André Barbosa , Vancini Rodrigo Luiz , Weiss Katja , Rosemann Thomas , Knechtle Beat , Andrade Marília dos Santos 
Published: November 2024

AbstractDuring an IRONMAN, the pacing strategy in the running
segment differs significantly between sexes. However, it is unknown if sex
affects the running pace in shorter triathlon events. This study compared the
pacing strategy between sexes during a 5-km running test performed following a
20-km cycle (sprint triathlon distances). Participants included 16 men (34.7±7.5
years) and 16 women (39.5±7.7 years). A cardiopulmonary exercise test to measure
maximal oxygen uptake (˙VO2max) and a functional threshold power
(FTP) were performed. Body composition was measured using a dual-emission X-ray
absorptiometry system. Participants cycled at 90% FTP for 20 km and ran as fast
as possible for 5 km. The total time spent cycling 20 km was shorter (~11%) in
male than in female athletes (p<0.001). Similarly, the time spent to run 5 km
was shorter in male (~11%) than in female athletes (p=0.006). Male athletes
performed the last 600 m of the 5km race significantly faster than the initial
meters (p<0.05). Female athletes performed the 5-km race without pace
variation (p>0.05). In conclusion, apart from the sex differences associated
with performance in short-term triathlon events, the pacing strategy was also
different.
Dural Closure Method Using Dural Suture and Polyglycolic Acid Nonwoven Fabric in the Endoscopic Endonasal Approach
Authors: Tanioka Daisuke , Natori Ikuya , Kobayashi Yusuke , Shimizu Katsuyoshi , Mizutani Tohru 
Published: November 2024

Abstract
Introduction The indications for surgery using the endoscopic endonasal approach (EEA) for tumors in the sella turcica and parasellar region are expanding. However, the risk of cerebrospinal fluid (CSF) leakage is also increasing. Therefore, to prevent postoperative CSF leakage, it is essential to use safe reconstruction methods for the sella turcica. A polyglycolic acid (PGA) nonwoven fabric is applied during dural reconstruction in neurosurgery. Nevertheless, its suitability for sella turcica reconstruction has not been validated.
Methods This study enrolled patients who underwent lesion resection using EEA, followed by sella turcica reconstruction using the dural closure method and the simple and robust suture and PGA (S-PGA) method. The outcomes of the surgery were compared with those of fat-based reconstructive procedures performed prior to the introduction of the S-PGA method.
Results In total, 325 patients underwent dural closure using the S-PGA method. The incidence rates of postoperative CSF leakage were 0.8% for the S-PGA method and 5.2% for the fat-filling method. This finding indicated significant improvement in the incidence rates of postoperative CSF leakage (P < 0.01). The S-PGA method did not affect radiogram interpretation of magnetic resonance imaging performed at 3 months after surgery due to the absence of intrasellar fat filling.
Conclusion The S-PGA method in EEA is a simple and reliable technique for decreasing the risk of postoperative CSF leakage to the greatest extent possible. Hence, it can be an excellent option for skull base reconstruction.
A Resilient and Reusable Ion-Tagged Cu(II) Catalyst for the Microwave-Assisted Synthesis of 2-(N-Arylamino)benzothiazoles
Authors: Balamurali M M , Chanda Kaushik , Ahmad Md Gulzar 
Published: November 2024

AbstractA simple, quick, and cost-effective protocol is presented herein for the sustainable synthesis of 2-(N-arylamino)benzothiazoles from 2-haloanilines and arylisothiocyanates by using an ionic liquid supported heterogeneous Cu(II) catalyst derived from N-methylimidazole. Compared to the use of homogeneous catalysts and additives, which require long reaction times and high reaction temperatures, this methodology has a broad substrate scope and proceeds in short reaction times to afford compounds in excellent yields under microwave irradiation. In addition, the catalyst can be extracted easily and is recyclable up to five times with no significant loss in catalytic activity.
Ocular Motor Mononeuropathies in Diabetes Mellitus: A Brief
Review
Authors: Pantazopoulos Dimitrios , Gouveri Evanthia , Papazoglou Dimitrios , Papanas Nikolaos 
Published: November 2024

AbstractOcular motor mononeuropathies affect cranial nerves III, IV and VI and are more
frequent in diabetes mellitus, with oculomotor nerve involvement being
predominant. This narrative brief review discusses the clinical manifestations,
diagnosis and management of ocular motor mononeuropathies in subjects with
diabetes. Clinical manifestations often include ptosis, diplopia, and
periorbital pain. Pupillary sparing is a characteristic of third nerve palsy.
Differential diagnosis may be challenging due to overlapping symptoms with nerve
palsies of other aetiologies. Treatment includes optimised glycaemic control and
management of vascular risk factors. Neuroprotective agents, mainly alpha-lipoic
acid and botulinum toxin A have been occasionally used, as well. Spontaneous
recovery is seen in many cases.