bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of a 12-gene expression signature to predict colon cancer prognosis
Authors: Sun Dalong , Chen Jing , Liu Longzi , Zhao Guangxi , Dong Pingping , Wu Bingrui , Wang Jun , Dong Ling 
Published: June 2018

A robust and accurate gene expression signature is essential to assist oncologists to determine which subset of patients at similar Tumor-Lymph Node-Metastasis (TNM) stage has high recurrence risk and could benefit from adjuvant therapies. Here we applied a two-step supervised machine-learning method and established a 12-gene expression signature to precisely predict colon adenocarcinoma (COAD) prognosis by using COAD RNA-seq transcriptome data from The Cancer Genome Atlas (TCGA). The predictive performance of the 12-gene signature was validated with two independent gene expression microarray datasets:GSE39582includes 566 COAD cases for the development of six molecular subtypes with distinct clinical, molecular and survival characteristics;GSE17538is a dataset containing 232 colon cancer patients for the generation of a metastasis gene expression profile to predict recurrence and death in COAD patients. The signature could effectively separate the poor prognosis patients from good prognosis group (disease specific survival (DSS): Kaplan Meier (KM) Log Rankp= 0.0034; overall survival (OS): KM Log Rankp= 0.0336) inGSE17538. For patients with proficient mismatch repair system (pMMR) inGSE39582, the signature could also effectively distinguish high risk group from low risk group (OS: KM Log Rankp= 0.005; Relapse free survival (RFS): KM Log Rankp= 0.022). Interestingly, advanced stage patients were significantly enriched in high 12-gene score group (Fisher’s exact testp= 0.0003). After stage stratification, the signature could still distinguish poor prognosis patients inGSE17538from good prognosis within stage II (Log Rankp = 0.01) and stage II & III (Log Rankp= 0.017) in the outcome of DFS. Within stage III or II/III pMMR patients treated with Adjuvant Chemotherapies (ACT) and patients with higher 12-gene score showed poorer prognosis (III, OS: KM Log Rankp= 0.046; III & II, OS: KM Log Rankp= 0.041). Among stage II/III pMMR patients with lower 12-gene scores inGSE39582, the subgroup receiving ACT showed significantly longer OS time compared with those who received no ACT (Log Rankp= 0.021), while there is no obvious difference between counterparts among patients with higher 12-gene scores (Log Rankp= 0.12). Besides COAD, our 12-gene signature is multifunctional in several other cancer types including kidney cancer, lung cancer, uveal and skin melanoma, brain cancer, and pancreatic cancer. Functional classification showed that seven of the twelve genes are involved in immune system function and regulation, so our 12-gene signature could potentially be used to guide decisions about adjuvant therapy for patients with stage II/III and pMMR COAD.
Retracted: IFITM3 promotes hepatocellular carcinoma invasion and metastasis by regulating MMP9 through p38/MAPK signaling
Authors: Min Jiaqi , Feng Qian , Liao Wenjun , Liang Yiming , Gong Chengwu , Li Enliang , He Wenfeng , Yuan Rongfa , Wu Linquan 
Published: June 2018

Interferon‐induced transmembrane protein 3 (IFITM3) has been shown to be overexpressed in multiple cancers. However, the role of IFITM3 in metastasis of hepatocellular carcinoma (HCC) is still poorly understood. In this study, we showed that IFITM3 was frequently overexpressed in HCC tissues compared with adjacent nontumor tissues. Overexpression of IFITM3 was significantly correlated with tumor metastasis and poor prognosis in HCC. Knockdown of IFITM3 dramatically decreased MMP9 expression and inhibited the invasion and metastasis of HCC in vitro and in vivo. Moreover, the upregulation of MMP9 rescued the decreased migration and invasion induced by the knockdown of IFITM3, whereas the knockdown of MMP9 decreased IFITM3‐enhanced HCC migration and invasion. Mechanistically, we found that IFITM3 regulates MMP9 expression through the p38/MAPK pathway. Taken together, we identified a novel IFITM3–p38/MAPK–MMP9 regulatory circuitry, the dysfunction of which drives invasive and metastatic character in HCC.
Cross Talk Between Autophagy and Apoptosis Contributes to ZnO Nanoparticle‐Induced Human Osteosarcoma Cell Death
Authors: He Guanping , Ma Yunlong , Zhu Ye , Yong Lei , Liu Xiao , Wang Peng , Liang Chen , Yang Chenlong , Zhao Zhigang , Hai Bao , Pan Xiaoyu , Liu Zhongjun , Liu Xiaoguang , Mao Chuanbin 
Published: June 2018

AbstractKilling osteosarcoma cells by zinc oxide nanoparticles (NPs) and its underlying subcellular mechanism are never studied. Here, it is found that the NPs induce cross talk between apoptosis and autophagy, which leads to osteosarcoma cell death. Specifically, the NP uptake promotes autophagy by inducing accumulation of autophagosomes along with impairment of lysosomal functions. The autophagy further causes the uptaken NPs to release zinc ions by promoting their dissolution. These intracellular zinc ions, together with those that are originally released from the extracellular NPs and flowed into the cells, collectively target and damage mitochondria to produce reactive oxygen species (ROS). Then the ROS inhibit cell proliferation by arresting S phase and trigger apoptosis by extrinsic and intrinsic pathways, ultimately leading to cell death. More importantly, suppression of the early stage autophagy restores cell viability by abolishing apoptosis whereas blockade of the late stage autophagy inversely enhances apoptosis. In contrast, inhibition of apoptosis shows a limited ability to restore cell viability but obviously enhance autophagy. Notably, cell viability is strongly ameliorated by the combination of inhibitors for both the late stage autophagy and the apoptosis. These findings provide a mechanistic understanding of the NP‐directed autophagy and apoptosis in osteosarcoma cells.
Producing Covalent Microarrays of Amine‐Conjugated DNA Probes on Various Functional Surfaces to Create Stable and Reliable Biosensors
Authors: Yousefi Hanie , Su Hsuan‐Ming , Ali Monsur , Filipe Carlos D. M. , Didar Tohid F. 
Published: June 2018

AbstractOligonucleotide‐based microarrays are ideal tools for genetic testing and diagnostics due to their stability, ease of synthesis, and high specificity for the target of interest. This study reports on the effectiveness of several coupling strategies to covalently attach single‐stranded nucleic acids to surfaces, focusing on the robustness of the attachment in various environmental conditions, such as pH and temperature. Various characteristics of DNA microarrays produced using amine‐conjugated DNA probes on five different functional surfaces, commonly used for immobilization of biomolecules, namely, epoxy, carboxyl, amine, aldehyde, and N‐hydroxysuccinimide‐coated substrates, are investigated. Immobilization efficiency, changes in surface energy, as well as the stability of the conjugated DNA upon exposure to various environmental conditions are measured. Finally, in order to study the postimmobilization viability of the developed biosensors, microarrays of synthetic RNA cleaving probes (DNAzyme) are immobilized onto these surfaces and their functionality is evaluated through their ability to detect Escherichia coli at various temperatures. Results show that epoxy‐coated plastic surfaces are most ideal for the creation of DNA‐based biosensing chips. This study provides a guideline for producing oligonucleotide‐based microarrays on glass and plastic substrates and can be used for developing microarray‐based biosensors, suitable for long‐term storage.
Fluorination‐Enhanced Ambient Stability and Electronic Tolerance of Black Phosphorus Quantum Dots
Authors: Tang Xian , Chen Hong , Ponraj Joice Sophia , Dhanabalan Sathish Chander , Xiao Quanlan , Fan Dianyuan , Zhang Han 
Published: June 2018

AbstractThe environmental instability and uneliminable electronic trap states in black phosphorus quantum dots (BPQDs) limit the optoelectronics and related applications of BPQDs. Here, fluorinated BPQDs (F‐BPQDs) are successfully synthesized by using a facile electrochemical exfoliation and synchronous fluorination method. The F‐BPQDs exhibit robust ambient stability and limited fluorination capability, showing a nonstoichiometric fluorination degree (DF) maximum of ≈0.68. Density functional theory calculations confirm that due to the edge etching effect of fluorine adatoms, the simulated F‐BPQDs become structurally unstable when DF surpasses the limit. Furthermore, the trap states of BPQDs can be effectively eliminated via fluorination to obtain a coordination number of 3 or 5 for fluorinated and unfluorinated phosphorus atoms. The results reveal that the air‐stable F‐BPQDs exhibit fluorine defect‐enhanced electronic tolerance, which is crucial for nanophotonics and nanoelectronics applications.
Recent Advances in Engineering the Stem Cell Microniche in 3D
Authors: Bao Min , Xie Jing , Huck Wilhelm T. S. 
Published: June 2018

AbstractConventional 2D cell culture techniques have provided fundamental insights into key biochemical and biophysical mechanisms responsible for various cellular behaviors, such as cell adhesion, spreading, division, proliferation, and differentiation. However, 2D culture in vitro does not fully capture the physical and chemical properties of the native microenvironment. There is a growing body of research that suggests that cells cultured on 2D substrates differ greatly from those grown in vivo. This article focuses on recent progress in using bioinspired 3D matrices that recapitulate as many aspects of the natural extracellular matrix as possible. A range of techniques for the engineering of 3D microenvironment with precisely controlled biophysical and chemical properties, and the impact of these environments on cellular behavior, is reviewed. Finally, an outlook on future challenges for engineering the 3D microenvironment and how such approaches would further our understanding of the influence of the microenvironment on cell function is provided.
SiC‐Doped Ge2Sb2Te5 Phase‐Change Material: A Candidate for High‐Density Embedded Memory Application
Authors: Guo Tianqi , Song Sannian , Song Zhitang , Ji Xinglong , Xue Yuan , Chen Liangliang , Cheng Yan , Liu Bo , Wu Liangcai , Qi Ming , Feng Songlin 
Published: June 2018

AbstractPhase‐change memory is one of the most attractive solutions for embedded applications, thanks to the low cost of integration with current manufacturing processes and the good scaling behavior. Despite intensive research efforts that are devoted to the modification of Ge2Sb2Te5 (GST) phase‐change material, the obtained improvements are still unsatisfactory for high‐performance applications. In this paper, SiC‐modification is first introduced to enhance the amorphous stability of GST without sacrificing its transition speed. Several enhancements can be demonstrated, including better thermal stability (10‐year data retention above 120 °C), lower RESET voltage (about 3.0 V at 20 ns pulse width), reduced volume change (smaller than 3.0%), and expected operation cycles (more than 1.0 × 105). According to the findings, the local bonding nature of Ge/Te atoms can be tuned by C/Si dopants through forming CGe and SiTe bonds. Apart from strengthening the atomic binding network for desirable data retention, the element interdiffusion is also effectively controlled and suppressed. Together with finer grains, smaller density change, and more uniform morphology, the modified structure transition finally contributes to the reduced switching voltage and long‐term endurance. Hence, it is envisaged that SiC‐doped GST with such advantages will give a competitive option for high‐density and high‐performance embedded memory.
Remote C−H Functionalization of 8‐Aminoquinolinamides
Authors: Khan Bhuttu , Dutta Himangsu Sekhar , Koley Dipankar 
Published: June 2018

Abstract8‐Aminoquinoline, a common structural motif present in many natural products, pharmaceuticals, agrochemicals, and important drugs has always been an attractive scaffold in synthetic organic chemistry. Recent years have witnessed the development of 8‐aminoquinoline as a ligand in transition‐metal‐catalyzed C−H functionalizations. Therefore, synthesis of functionalized 8‐aminoquinolines is of great importance and rapid generation of functionalized quinolines could be achieved by direct C−H functionalization. In this regard, since the seminal report of distal C5‐H halogenations of quinoline, diverse C−X bond formation reactions have been published. This review is focused on the C5‐H functionalization of 8‐aminoquinolinamide derivatives, including not only C−C bond formation reactions but also carbon–heteroatom bond formations such as C−X (X=F, Cl, Br, I) C−S, C−Se, C−N, C−O, and C−P. In most cases, the C5 position is activated by a single electron transfer method employing either metal catalysts, photocatalysts, or metal‐free conditions.
Shortcomings in the diagnosis of chronic rhinosinusitis: evaluating diagnosis by otolaryngologists and primary care physicians
Authors: Xiao Christopher C. , Anderson Mitchell , Harless Lucas D. , Liang Jonathan 
Published: June 2018

BackgroundChronic rhinosinusitis (CRS) is a prevalent illness in the United States that accounts for 18–22 million physician visits annually. The American Academy of Otolaryngology–Head and Neck Surgery (AAO‐HNS) has defined diagnostic criteria, but a recent study demonstrated that nearly all patients diagnosed by nonspecialists did not meet these criteria. In this study we aimed to evaluate the diagnostic rate of CRS by primary care physicians and otolaryngologists.MethodsWe retrospectively reviewed a random sample of adult patients diagnosed with CRS in 2016, based on ICD‐10 codes from primary care and otolaryngology departments. Patients with previous CRS diagnosis, previous sinus surgery, and related comorbidities were excluded.ResultsA total of 502 patients with a new CRS diagnosis were analyzed (308 from primary care, 194 from otolaryngology). The percentage of diagnoses meeting the criteria was significantly higher from otolaryngology (28.9% vs 0.97%, p < 0.0001), but was low in both cohorts. Symptom duration <12 weeks was higher in primary care (81.6% vs 53.6%, p < 0.0001), as was lack of evidence of inflammation (97.4% vs 50.0%, p < 0.0001). Having <2 of the required symptoms was significantly higher in otolaryngology (63.8% vs 50.8%, p = 0.013). The most commonly unevaluated symptom was decreased sense of smell (97.7% in primary care, 69.1% in otolaryngology encounters).ConclusionCRS diagnoses commonly do not meet the diagnostic criteria outlined by the AAO‐HNS in both primary care and otolaryngology. As a specialty, we should aim to improve our adherence to the guidelines and educate our primary care colleagues to better identify patients with CRS and initiate appropriate treatment.
Synthesis and solid‐state structures of t‐Bu3Ga–EPh3 Lewis acid–base adducts
Authors: Heckel Alla , Bendt Georg , John Lukas , Wölper Christoph , Schulz Stephan 
Published: June 2018

Three Lewis acid–base adducts t‐Bu3Ga–EPh3 (E = P 1, As 2, Sb 3) were synthesized by reactions of Ph3E and t‐Bu3Ga and characterized by heteronuclear NMR (1H, 13C (31P)) and IR spectroscopy, elemental analysis and single crystal X‐ray diffraction. Their structural parameters are discussed and compared to similar t‐Bu3Ga adducts. The strength of the donor‐acceptor interactions within 1–3 was investigated in solution by temperature‐dependent 1H NMR spectroscopy and by quantum chemical calculations.
Valence‐Tuned Lithium Titanate Nanopowder for High‐Rate Electrochemical Energy Storage
Authors: Widmaier Mathias , Pfeifer Kristina , Bommer Lars , Presser Volker 
Published: June 2018

AbstractThe front cover artwork highlights work from the Robert Bosch GmbH and the INM–Leibniz Institute for New Materials (Germany). The image illustrates the effect of hydrogen annealing on lithium titanate: creating oxygen vacancies in the crystal structure not only makes the white powder become dark blue, but also electrically much more conductive. As a result, lithium titanate allows for ultrahigh charge and discharge rates of lithium‐ion batteries. Read the full text of the article at 10.1002/batt.201700007.
A sensitive SERS quantitative analysis method for Ni2+ by the dimethylglyoxime reaction regulating a graphene oxide nanoribbon catalytic gold nanoreaction
Authors: Liang Aihui , Li Xin , Zhang Xinghui , Wen Guiqing , Jiang Zhiliang 
Published: June 2018

AbstractThe nanogold reaction between HAuCl4 and trisodium citrate (TCA) proceeded very slowly at 60°C in a water bath. The as‐prepared graphene oxide nanoribbons (GONRs) exhibited strong catalysis during the reaction to form gold nanoparticles (Au NPs) and appeared as a strong surface‐enhanced Raman scattering (SERS) peak at 1616 cm−1 in the presence of the molecular probe Victoria blue 4R (VB4r). With increase in GONR concentration, the SERS peak increased due to increased formation of Au NPs. Upon addition of dimethylglyoxime (DMG) ligand, which was adsorbed onto the GONR surface to inhibit GONR catalysis, the SERS peak decreased. When Ni2+ was added, a coordination reaction between DMG and Ni2+ took place to form stable complexes of [Ni (DMG)2]2+ and the release of free GONR catalyst that resulted in the SERS peak increasing linearly. A SERS quantitative analysis method for Ni2+ was therefore established, with a linear range of 0.07–2.8 μM, and a detection limit of 0.036 μM Ni2+.
Highly Stabilized Zinc–Air Batteries Based on Nanostructured Co3O4 Composites as an Efficient Bifunctional Electrocatalyst
Authors: Nie Qi , Cai Yixiao , Xu Nengneng , Peng Luwei , Qiao Jinli 
Published: June 2018

AbstractThe front cover artwork is provided by Prof. Jinli Qiao's research group from Donghua University (China). The image shows an as‐prepared composite material consisting of Co3O4 with two carbon materials [i.e. carbon nanotubes (CNTs) and mesoporous carbon] with excellent oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) performance. The composite shows great potential and practical implementation for rechargeable energy devices in electric vehicles are envisioned. Read the full text of the Article at 10.1002/celc.201800159.
Differential Modulation of the Aggregation of N‐Terminal Truncated Aβ using Cucurbiturils
Authors: Li Gao , Yang Wu‐Yue , Zhao Yu‐Fen , Chen Yong‐Xiang , Hong Liu , Li Yan‐Mei 
Published: June 2018

AbstractModulating the aggregation of Aβ has long been considered to be one of the potential methods to treat Alzheimer's disease (AD). It has been found that different Aβ species, including N‐terminal truncated or/and modified Aβ, co‐exist in the brain of AD patients. Yet, there is currently little detailed work about the specific modulation of these Aβ species which hinders us to understand their roles in patients’ brain. Using thioflavin T (ThT) kinetics and transmission electron microscope, here we showed that cucurbit[7]uril and cucurbit[8]uril could inhibit the aggregation of both Aβ4‐40 and Aβ1‐40 through host‐guest interactions. Chemical kinetics analysis suggested that this happened through inhibiting the elongation process by binding with fibril ends. In addition, cucurbiturils showed greater capability on the inhibition of Aβ4‐40 than Aβ1‐40, which was possibly due to the N‐terminal phenylalanine residue of Aβ4‐40. Our work provided new insights for the development of host–guest chemistry based inhibitors for the aggregation of different Aβ species.
Pd(II)‐Catalyzed Phosphorylation of Enamido C(sp2)–H Bonds: A General Route to β‐Amido‐vinylphosphonates
Authors: Qiao Baokun , Cao Hao‐Qiang , Huang Yin‐Jun , Zhang Yue , Nie Jing , Zhang Fa‐Guang , Ma Jun‐An 
Published: June 2018

AbstractOrganophosphorus compounds are essential structures in modern pharmaceutical, agrochemical, and material sciences. The development of new and efficient methods for the synthesis of C–P bonds has been an important focus of research. We herein report a Pd‐catalyzed enamido C(sp2)–H phosphorylation for direct construction of C–P bonds under simple and convenient conditions without the need for additional ligands or directing groups. The present reaction can tolerate a wide range of functional groups, and furnish a variety of phosphorylation products including tetrasubstituted‐vinyl β‐aminophosphonates that are otherwise difficult to access. This protocol was also exemplified into the late‐stage modification of bioactive natural products and was suitable for large‐scale synthesis.
OW‐SVM: Ontology and whale optimization‐based support vector machine for privacy‐preserved medical data classification in cloud
Authors: Karlekar Nandkishor P. , Gomathi N 
Published: June 2018

SummaryCloud is a multitenant architecture that allows the cloud users to share the resources via servers and is used in various applications, including data classification. Data classification is a widely used data mining technique for big data analysis. It helps the learners to discover hidden data patterns by training massive data collected from the real world. Because this trained model is the private asset of an entity, it should be protected from all other noncollaborative entities. Therefore, it is essential to take effective measures to preserve the confidential data. The objective of this paper is to preserve the privacy of the confidential data in the cloud environment by introducing the medical data classification method. In view of that, this paper presents a method for medical data classification using a novel ontology and whale optimization‐based support vector machine (OW‐SVM) approach. Initially, privacy‐preserved data are developed adopting Kronecker product bat approach, and then, ontology is built for the feature selection process. Ontology and whale optimization‐based support vector machine is then proposed by integrating ontology and whale optimization algorithm into SVM, in which ontology and whale optimization algorithm is used for the feasible selection of kernel parameters. The experiment is done using 3 heart disease datasets, such as Cleveland, Switzerland, and Hungarian. In a comparative analysis, the performance of the OW‐SVM approach is compared with that of K‐nearest neighbor, Naive Bayes, decision tree, SVM, and OW‐SVM, using accuracy, sensitivity, specificity, and fitness, as the evaluation metrics. The OW‐SVM approach could achieve maximum performance with accuracy of 83.21%, the sensitivity of 91.49%, specificity of 73%, and fitness of 81.955, outperforming existing comparative techniques.
Error performance of Uncoded Space Time Labelling Diversity in spatially correlated Nakagami‐q channels
Authors: Patel Sulaiman Saleem , Quazi Tahmid , Xu Hongjun 
Published: June 2018

SummaryGreater spectral efficiency has recently been achieved for Uncoded Space Time Labelling Diversity (USTLD) systems by increasing the number of antennas in the transmit antenna array. However, due to constrained physical space in hardware, the use of more antennas can lead to degradation in error performance due to correlation. Thus, this paper studies the effects of spatial correlation on the error performance of USTLD systems. The union bound approach, along with the Kronecker correlation model, is used to derive an analytical expression for the average bit error probability (ABEP) in the presence of Nakagami‐q fading. This expression is validated by the results of Monte Carlo simulations, which shows a tight fit in the high signal‐to‐noise ratio (SNR) region. The degradation in error performance due to transmit and receive antenna correlation is investigated independently. Results indicate that transmit antenna correlation in the USTLD systems investigated (3 × 3 8PSK, 2 × 4 16PSK, 2 × 4 16QAM, and 2 × 4 64QAM) causes a greater degradation in error performance than receive antenna correlation. It is also shown that 2 × 4 USTLD systems are more susceptible to correlation than comparable space‐time block coded systems for 8PSK, 16PSK, 16QAM, and 64QAM.