bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of TPST1 as a Promising Prognostic Biomarker in Head and Neck Squamous Cell Carcinoma
Authors: Chen Shuai , Chen Fei , Huang Shanying , Jia Wenming , Qian Ye 
Published: December 2024

Abstract

Head and neck squamous cell carcinoma (HNSC) is one of the most common cancers in the world, with poor prognosis and lack of effective biomarkers for early diagnosis. Tyrosylprotein sulfotransferase 1 (TPST1) has been implicated in a variety of biological processes and tumor progression. The differential expressions of TPST1 in human tumors and the clinical prognosis in HNSC were examined via multiple bioinformatics approaches, and verified in human HNSC tissues. In addition, a series of functional assays were performed to explore the biological role of TPST1 in HNSC.Our findings revealed that TPST1 expression in HNSC samples was considerably upregulated than that of normal tissues. High TPST1 expression was linked with clinicopathological characteristics and unfavorable prognosis of HNSC patients. Genetic mutation of TPST1 was involved in the development of HNSC. We identified TPST1 related co-expressed genes, which were mainly enriched in the Wnt signaling pathway associated with the tumorigenesis, leading to a poor prognosis of HNSC patients. we analyzed the correlation between TPST1 and pathways, and verified by experiments.The results showed that TPST1 is an oncogene and may function as a potential prognosis biomarker for HNSC patients, suggesting that TPST1 could be a promising therapy target against HNSC.
Synergistic effects of osseointegration and multifunctional properties: Mechanical and electrical performance of functionally graded ceramic composites before and after soaking in SBF for bone healing applications
Authors: Youness Rasha A. , Mostafa Essam B. , Taha Mohammed A. 
Published: December 2024

Abstract

This work presents the creation of functionally graded composite (FGC) intending to enhance bone healing, as traditional bone repair materials typically do not possess the necessary qualities for perfect healing. In this context, the FGC was fabricated by layering five layers of hydroxyapatite (HA), silicon carbide (SiC), and copper oxide (CuO) nanoparticles. Analysis was conducted using characterization techniques such as X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM). Furthermore, the osseointegration ability of all prepared FGC layers was assessed using simulated body fluid (SBF) and investigation by FESEM. The physical, mechanical, electrical, and dielectric properties were measured before and after soaking in the SBF solution. Additionally, the antibacterial effect and biocompatibility of these layers were evaluated. Sintered layers exhibit porosity values ​​ranging from 5–10%, similar to compact bone, which is essential for effective osseointegration. FESEM images showed good bioactive behavior across all FGC layers. Increasing the proportions of SiC and CuO improved the compressive strength to match cortical bone, reducing compression shielding. In addition, these additives significantly enhanced electrical conductivity and reduced dielectric properties, which are vital factors for bone regeneration. Significantly, soaking the prepared layers with SBF did not affect the mechanical properties, which indicates that there is no conflict between the biological activity of these layers and their mechanical properties. However, their electrical and dielectric properties changed slightly due to their soaking in the SBF solution. Notably, the sample with the highest SiC and CuO content exhibited a 75% reduction in weight loss. Importantly, CuO inclusion led to a considerable improvement in the antibacterial efficacy of the FGC layers without a noticeable cytotoxic effect. These findings indicate that the prepared FGC and its layers have promising multifunctional performance and are a suitable candidate for bone healing applications.
Comparison of the Efficacy of Robot-assisted Total Knee Arthroplasty in Patients with Knee Osteoarthritis with Varying Severity Deformity
Authors: Xing Pengfei , Qu Junsong , Feng Shuaijing , Guo Jiarong , Huang Tao 
Published: December 2024

Abstract

AIM: To evaluate the clinical efficacy and imaging outcomes of a domestically produced orthopedic surgical robot for total knee arthroplasty and to explore its applicability in patients with varying degrees of deformity.
Methods: This study retrospectively included 120 patients who underwent TKA at our hospital between February 2023 and June 2024. The patients were divided into a control group (conventional TKA surgery) and an observation group (robot-assisted TKA surgery), with 60 patients in each group. On the basis of different lower extremity alignment angles, each group was further subdivided into mild deformities (HKA deviation < 10°) and significant deformities (HKA deviation ≥ 10°). Preoperative and postoperative HKA angles, range of motion (ROM), visual analog scale (VAS) pain scores, and Knee Society (AKS) scores were recorded for both groups. Postoperative measurements included the posterior tibial slope angle (PSA), femoral coronal component angle (FFC), tibial coronal component angle (FTC), and femoral sagittal component angle (LFC), as well as the incidence of abnormal values for each angle, which were analyzed statistically.
Results: The operation time in the observation group was longer than that in the control group, but intraoperative blood loss was significantly lower in the observation group (P < 0.05). Postoperatively, the differences in the HKA and PSA angles and the incidence of abnormal values were significantly greater in the robot-assisted group than in the control group (P < 0.05). The other indicators did not significantly differ between the two groups. (P > 0.05). For patients with mild preoperative deformities, those in the robot-assisted group had significantly greater postoperative HKA angle deviations and rates of postoperative HKA angle outliers than did those in the conventional group. For patients with significant deformities, the robot-assisted group presented greater postoperative HKA and PSA angle deviations than did the control group; the postoperative HKA and PSA angle outlier rates were significantly lower in the robot-assisted group (P < 0.05).
Conclusion: The TINAVI robotic system demonstrated superior safety and efficacy in TKA surgery. Compared with conventional TKA, the robot-assisted system achieved significantly better outcomes in terms of prosthesis implantation accuracy and lower extremity alignment, with a particular advantage in patients with severe limb alignment deformities.
Peripheral players and gut bacteria modulate inflammatory bowel disease phenotype in patients with primary sclerosing cholangitis
Authors: Santos André , Pires David , Marques Vanda , Alesina Nicole , Herraez Elisa , Roudnický Pavel , Rodrigues Pedro , Godinho-Santos Ana , Bravo Ana , Gouveia Catarina , Saraiva Susana , Correia Luís , Crespo Ricardo , Silva João da , Cravo Marília , Potesil David , Zdráhal Zbyněk , Bañales Jesús , Marín José , Torres Joana , Rodrigues Cecília 
Published: December 2024

Abstract

Background & Aims:
Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease often associated with underlying inflammatory bowel disease (IBD). We aimed to study how the phenotype in PSC patients compares to IBD alone and its association with altered inflammatory immune responses.
Methods
A case-control study was conducted involving a cohort of 75 patients, including 16 with PSC (14 with concomitant IBD), 39 with IBD alone, and 20 control individuals. Fecal gut microbiota, colonic tissue proteomics and immune-related gene expression, serum proteomics and targeted metabolomics were analyzed.
Results
Intestinibacter taxa were increased in patients with PSC. Proinflammatory mRNA markers TWIST1, COX2, IL-8, and CCL2, and pro-oncogenic markers LGR5 and SNAIL were upregulated in colonic tissue from PSC patients. Additionally, a unique proinflammatory proteomic signature, elevated glycochenodeoxycholic acid (GCDCA), and increased miR-21 were identified in serum from PSC patients. Co-incubation of human-derived monocytes with miR-21 and GCDCA reproduced the inflammatory profile observed in PSC patients.
Conclusions
These findings suggest an interplay between gut microbiota dysbiosis and the proinflammatory peripheral immune response contributing to the unique PSC phenotype
LncRNA-mediated High Expression of TRIP13 Correlates with Poor Prognosis and Tumor Immune Infiltration of Hepatocellular Carcinoma
Authors: Cao Ya-wen , Ying Jiaxin , Ye Yong-qing 
Published: December 2024

Abstract

Background:
Hepatocellular carcinoma (HCC) accounts for approximately 90% of primary liver cancers and is charactered by poor prognosis. The identification of potential prognostic biomarkers is essential for HCC patients.
Methods: In this study, Gene Expression Profiling Interative Analysis (GEPIA) online tool was used to the different expression of TRIP13 and has-miR-29c-3p axis between tumor samples and normal samples in multiple cancers including hepatocellular carcinoma (HCC). The oncological value of TRIP13 and has-miR-29c-3p axis in cancer was revealed by Kaplan-Meier analysis. The upstream miRNAs of TRIP13 were predicted by the starBase database and Cytoscape software.
Results:
In this work, we identified that compared to normal tissues, the expression of TRIP13 was high in tumor samples by The Cancer Genome Atlas (TCGA) and Genotypic-Tissue Expression (GTEx) data. We further revealed that TRIP13 may be a potential oncogene in HCC. Subsequently, hsa-miR-29c-3p was responsible for TRIP13 overexpression, which was identified by expression analysis, correlation analysis and survival analysis of the target gene. Further, hsa-miR-29c-3p was associated with the poor prognosis of cancer patients. Moreover, the expression level of TRIP13 was positively correlated with tumor immune cell infiltration with high infiltrating level of B cells, CD4+ T cells, macrophages, exhausted CD8+ T cells and immune checkpoints including PD1, PD-L1 and CTLA-4.
Conclusions:
In general, our findings reveal that TRIP13 and hsa-miR-29c-3p promotes the malignant development of hepatocellular carcinoma, which is a potential new therapeutic target and a prognostic biomarker for HCC.
Preoperative hypoalbuminemia is associated with postoperative short- and long-term renal function deterioration in patients who underwent radical cystectomy for bladder cancer
Authors: Song Byeongdo , Lee Hakmin , Lee Sangchul , Hong Sung Kyu , Byun Seok-Soo , Oh Jong Jin 
Published: December 2024

Abstract

Radical cystectomy (RC), which is the standard of care for muscle-invasive and high-grade non-invasive bladder cancer, is accompanied with postoperative renal function detoriation. We aimed to evaluate the effect of hypoalbuminemia on the postoperative renal function decline after RC. A total of 272 patients with estimated glomerular filtration rate (eGFR) ≥ 60 mL/minute/1.73 m2 who underwent RC between October 2003 and December 2020 were included. Acute kidney injury (AKI) was defined according to Kidney Disease Improving Global Outcomes (KDIGO) criteria, while postoperative chronic kidney disease (CKD) progression was defined as eGFR < 60 mL/minute/1.73 m2 at ≥ 3 months after RC. In our cohort, 20 (7.4%) and 99 patients (36.4%) experienced AKI and CKD progression postoperatively, with a median follow-up period of 51.5 months. The median preoperative serum albumin level and eGFR were 4.1 g/dL and 82.0 mL/minute/1.73 m2, respectively. Preoperative hypoabluminemia < 4.1 g/dL was associated with postopeative AKI (odd ratio [OR] 3.37, p = 0.029) and CKD progression (OR 2.64, p < 0.001) after adjusting other factors. The patients with hypoalbuminemia < 4.1 g/dL are associated with short- and long-term renal function decline after RC, suggesting that neoadjuvant chemotherapy might be considered prior to RC for these patients.
BH3 Mimetics Augment Cytotoxic T Cell Killing of Acute Myeloid Leukemia via Mitochondrial Apoptotic Mechanism
Authors: Yee Cassian , Saxena Kapil , Ryu Esther , Hung Shao-Hsi , Singh Shailbala , Zhang Qi , Zeng Zhihong , Wang Zhe , Konopleva Marina 
Published: December 2024

Abstract

Adoptive cell therapy (ACT) can address an unmet clinical need for patients with relapsed/refractory acute myeloid leukemia (AML), but its effect is often modest in the setting of high tumor burden. In this study, we postulated that strategies to lower the AML apoptotic threshold will augment T cell killing of AML cells. BH3 mimetics, such as venetoclax, are a clinically approved class of compounds that predispose cells to intrinsic apoptosis by inhibiting anti-apoptotic mitochondrial proteins. We explored the anti-leukemic efficacy of BH3 mimetics combined with WT1-specific CD8 + T cells on AML cell lines and primary samples from patients with a diverse array of disease characteristics to evaluate if lowering the cellular apoptotic threshold via inhibition of anti-apoptotic mitochondrial proteins can increase leukemic cell sensitivity to T cell therapy. We found that the combination approach of BH3 mimetic and CD8 + T cells led to significantly increased killing of established AML lines as well as of adverse-risk primary AML leukemic blast cells. In contrast to the hypothesis that enhanced killing would be due to combined activation of the intrinsic and extrinsic apoptotic pathways, we found that CTL-mediated killing of AML cells was accomplished primarily through activation of the intrinsic/mitochondrial apoptotic pathway. This highly effective combinatorial activity due to convergence on the same apoptotic pathway was conserved across multiple AML cell lines and primary samples, suggesting that mitochondrial priming may represent a novel mechanism of optimizing adoptive cell therapy for AML patients.
Exposure to No 2 in Children’s Parks During a High Pollution Episode Based on Mesoscale Simulations
Authors: Simón-Moral Andrés , Herranz-Pascual Karmele , Padró Ales , Lertxundi Aitana , Yurrebaso Lexuri , Martilli Alberto 
Published: December 2024

Abstract

Air pollution is one of the greatest environmental risk factors for human health, especially worrying in urban areas, where children are one of the most vulnerable sectors. Continuous information of exposure to different air pollutants is therefore needed, especially in areas where children spend most of their time, as urban parks. In this study, we use 500 m horizontal resolution mesoscale simulations to assess children exposure to NO2 during a high pollution episode in five urban parks across Vitoria-Gasteiz. Children living at a maximum 5-minute walking time from each park is considered for exposure calculation. Results show that four parks exceed current hourly limit values, and all of them exceed the daily limits defined by the World Health Organization 2021 guideline. The use of hourly values instead of the daily mean for pollutant exposure calculation shows that the latter underestimates morning exposure and overestimates evening exposure. This result highlights the need for high time resolution to achieve better exposure assessment. The spatial resolution used in this study also shows the capacity to differentiate between parks, improving the exposure estimates respect the use of official air quality stations. This methodology provides continuous information to assess short term exposure, especially important in a high pollution episode, and allows for pollution gaps filling for long term exposure studies.
Acceptable survival of one-and-a-half-stage exchange arthroplasty with cement augmentation for periprosthetic joint infection
Authors: Song Sang Jun , Kim Young Kook , Kim Jin Woo , Park Cheol Hee 
Published: December 2024

Abstract

Purpose: To compare the aseptic survival of 1.5-stage exchange arthroplasty for periprosthetic joint infection (PJI) with and without cement augmentation using an autoclaved original femoral component and a new polyethylene insert (PE).
Methods: Among 1.5-stage exchange arthroplasties without reinfection, 42 cases with cement augmentation (Group C) and 88 cases without cement augmentation (Group N) were retrospectively reviewed. The cement augmentation technique was applied when joint stability could not be achieved using the thickest PE. Demographics were not significantly different between the groups. The hip-knee-ankle angle (HKA) and component positions were measured; the proportions of appropriate HKA and component positions (HKA≤0±3°, α≤95±3°, β≤90±3°, γ≤3±3°, and δ≤87±3°) were investigated. Survival and failure rates were analyzed, and failure was defined as reoperation owing to aseptic failure. The risk factors affecting survival were investigated in terms of demographics, cement augmentation, inappropriate HKA and component position for all patients in both groups.
Results: Although the average HKA and component positions were not different, appropriate proportion of coronal and sagittal PE position were significantly lower in the group C (appropriate β, 57.1% vs 90.9%; appropriate δ, 38.1% vs 77.3%; p<0.001, respectively). The 1-, 2-, and 5-year survival rates were 76.2%, 71.4%, and 71.4% in group C and 89.8%, 86.4%, and 81.6% in group N, respectively (p=0.063). The failure rate was not significantly different, despite the higher failure rate in group C (28.6% vs. 18.2%, p=0.253). The significant factor affecting the survival was only the inappropriate coronal positioning of the PE (β>90±3°) in overall cases (odds ratio=2.957, p=0.016).
Conclusion: The cement augmentation technique used to compensate for instability allows acceptable survival in 1.5 stage exchange arthroplasty. However, care is required when using this technique in the appropriate PE position to ensure favorable survival.
Major Glacial Cycle Change was not Driven by Removal of Slippery Sediment
Authors: Drew Matthew , Tarasov Lev 
Published: December 2024

Abstract

Past sea level change informs our predictions of future sea level rise [1] and, more broadly, the large scale climate changes that drove ice sheet growth and decay [2]. Approximately one million years ago, the relationship between orbitally-driven insolation and glacial cycle sea level change shifted – small, 40 thousand year cycles ceded to large, 100 thousand year cycles[3]. A leading hypothesis for this Mid-Pleistocene Transition is that the North American ice sheet grew resilient to deglaciation by removing its bed of slippery regolith, exposing the high friction bedrock underneath[4]. Our data constrained numerical experiments show that the North American ice sheet exposed bedrock over an area similar to present day by 1.5 million years ago, well before the Mid-Pleistocene Transition began. Thicker pre-glacial regolith does not simply delay its removal and thus the transition – it limits ice-ocean interaction by infilling shallow marine areas while cooling via the elevation-lapse rate feedback. Topographic changes over previously glaciated domains play a critical role in early Pleistocene glaciation – enabling larger ice sheets during warmer periods. This highlights the important and under-appreciated role topographic change plays in past sea
ISEBC: Using A Novel Breast Cancer Tertiary Lymphoid Structures Signature To Build An Immune-favourable Status Evaluator For Breast Cancer
Authors: Fan Xiaokai , Yu Xuan , Chen Liang 
Published: December 2024

Abstract

Tertiary lymphoid structures (TLSs), as a special type of immune-infiltrated region in tumor tissues, plays an important role in benefiting from immunotherapy or improving immune state of breast cancer patient. However, breast cancer-specific TLS signature gene sets are still lacking. Therefore, we extracted gene features with machine learning algorithm LightGBM, and differential expression genes with statistical test on multiple spatial transcriptome datasets, to finally obtain a novel breast cancer-specific TLS gene set (NBCTS). Compared with previous gene sets, it has stronger characterization ability of TLS in breast cancer samples. Since TLS have unique immune characteristics, we classified three different immune states using this gene set for breast cancer patients and get an immune state. To better facilitate evaluating this immune statuses of breast cancer patients or samples, we developed a user-friendly web tool (Immune State Evaluator for Breast Cancer, (ISEBC),www.omegene.tech:3838/ISEBC) to make it more convenient for researchers and clinicians to use.
Longitudinal analysis of peripheral blood immune status and prognosis dynamic prediction for advanced colon cancer with first-line chemotherapy
Authors: Wang Fan , Li Chun-xia , Liu Dong-Li , Zhao Jin-Yan , Wang Qing-Guo , Li Qi , Zhang Tao , Cai Xun 
Published: December 2024

Abstract

Background
The prognostic evaluation of advanced CRC patients places increased importance on longitudinal peripheral blood immune status. This study aimed to identify prognosis associated longitudinal immune markers and construct dynamic prognostic models for advanced CRC patients with first-line chemotherapy.
Methods
Metastatic CRC patients treated with standard first-line palliative chemotherapy were retrospectively collected at Shanghai General Hospital from May 2013 to May 2020. Lymphocyte subsets, inflammatory indices, and tumor markers in peripheral blood were repeatedly assessed before each chemotherapy cycle. Joint models were used to identify significant longitudinal prognostic markers. A dynamic prognostic model was established using random forests for time-dependent predictors, and internally validated using 10-fold cross-validation.
Results
Increased levels of CRP, CEA, CA199, and IL-6, as well as the CD4 + CD29 + cell proportion and the CD4 + CD45RO+/CD4 + ratio were identified as significant risk factors for overall survival (OS) in metastatic CRC patients. Conversely, the increased levels of CD3-CD19 + cell proportion and the CD4 + CD45RA+/CD4 + ratio were identified as favorable factors for OS. A dynamic prognostic model demonstrated good discriminative ability, with AUC values of 0.827, 0.787, 0.726, and 0.693 for 2-, 3-, 4-, and 5-year predictions, respectively. A high ratio of CD4 + CD45RA+/CD4 + before the 3rd to 4th chemotherapy cycle was associated with significantly better OS. Normal CRP and IL-6 levels in the early phase of first-line chemotherapy indicated a good prognosis.
Conclusion
This study highlights the prognostic significance of measuring longitudinal immune status in advanced CRC patients and provides a novel dynamic prediction tool for clinical practice.
Comparison of ground and aero magnetic anomalies: Two-dimensional crustal modelling of the Eastern Pontides (Türkiye) from integrated magnetic and gravity data
Authors: ÖZKAPTAN Murat , ÖZKAPTAN Tuğçe , BABACAN Ali Erden 
Published: December 2024

Abstract

Magnetic and gravity surveys are frequently used by earth scientists, given their ease of use and ability to provide rapid results. Various data platforms have integrated these data obtained from ground, sea, and airborne measurements on a global scale. The combining process reduces data resolution in some cases. In this study, aero and ground magnetic data in the Eastern Pontides were analysed to test this situation. The two magnetic data normalized for comparison show great similarities. It has also been suggested that carrying out ground magnetic survey at intervals of at most 10 km is sufficient for modelling large-scale deep structures. They were also used together with global gravity data to model the crustal structure of the Eastern Pontides. The results were compared with crustal models using gravity and seismological data in the literature. Contrary to the monotonic crustal thickness increase from north to south suggested in the literature, a gravity increase of approximately 50 mGal was determined in the south of the Eastern Pontides (axial zone) and it was suggested that the crust became thinner in this region. In addition, the crustal thickness variation was determined more clearly with the contribution of the two magnetic data sources.