bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment and characterization of a fin tissue cell line derived from silver pomfret, Pampus argenteus
Authors: Li Jianhuan , Jia Peng , Chen Xueji , Lai Mingyan , Jin Fanming , Liu Wei , Yi Meisheng , Jia Kuntong 
Published: August 2019

AbstractA cell line (PaF) derived from the fin tissue of silver pomfret (Pampus argenteus) was established and characterized in this study. The cell line has been subcultured for more than 50 times in Dulbecco's modified Eagle's medium (DMEM) containing 15% foetal bovine serum (FBS) since the initial primary culture. PaF cells grew well at temperatures from 24°C to 28°C in DMEM supplemented with 15% FBS. Partial amplification and sequence analysis of the cytochrome B gene indicated that PaF originated from silver pomfret. Cytogenetic analysis demonstrated that the modal chromosome number was 48. A significant cytopathic effect was observed in PaF cells during viral haemorrhagic septicaemia virus (VHSV) infection, and the VHSV replication was confirmed by qRT‐PCR and viral titre assays. In contrast, PaF cells were resistant to red‐spotted grouper nervous necrosis virus infection. Moreover, PaF cells could respond to VHSV and lipopolysaccharide treatments, as indicated by the expression of immune‐related genes, TLR5 and TLR9. In conclusion, the establishment of PaF cell line will provide an appropriate in vitro tool for the study of mechanisms of pathogen–silver pomfret interaction.
“We Came for the Cartilla but We Stayed for the Tortilla”: Enlisting in the Military as a Form of Migration for Zapotec Men
Authors: Sandoval‐Cervantes Ivan 
Published: August 2019

ResumenEn años recientes se ha discutido de manera extensa el papel del ejército Mexicano. Sin embargo, aún sabemos poco de las vidas de los soldados mismos. Este artículo analiza las experiencias de hombres Zapotecos que son parte del ejército Mexicano y establece dos puntos: (1) La relación entre comunidades indígenas y el ejército Mexicano se entrelaza con fenómenos políticos y económicos más amplios; y (2) las experiencias de soldados indígenas deben de ser analizadas no sólo en términos ocupacionales, sino como una forma de migración. En relación a este último punto, concluyo que existen conexiones y paralelismos importantes entre la experiencia de enlistarse en el ejército y de migrar a los Estados Unidos.
Two languages, one variable? Pharyngeal realizations among Arabic–Hebrew bilinguals
Authors: Gafter Roey J. , Horesh Uri 
Published: August 2019

AbstractLinguistic features that index ethnic identities often originate in another language spoken by the same community, often involving the introduction of exogenous features absent from the mainstream variety. We examine the more unusual situation of Hebrew spoken by Arabic–Hebrew bilingual Palestinians, where the pharyngeal consonants, which occur in both Arabic and Hebrew monolingual varieties, serve as ethnically linked sociolinguistic variables. Drawing on sociolinguistic interviews with such bilinguals in Jaffa, we demonstrate that while Palestinians’ use of pharyngeals in Hebrew is commonly perceived as transfer from Arabic, their linguistic and social conditioning does not support a transfer account. Rather, for these speakers, pharyngeals are a socially meaningful resource simultaneously linked to Arab identity and to a rich indexical field in Hebrew. While the pharyngeals pattern linguistically as Hebrew features, our findings illustrate that, from a social meaning perspective, sociolinguistic features need not be unambiguously associated with a single language.
Effects of pegylated interferon‐α based therapies on functional cure and the risk of hepatocellular carcinoma development in patients with chronic hepatitis B
Authors: Ren Hong , Huang Yan 
Published: August 2019

AbstractChronic hepatitis B virus (HBV) infection continues to pose a serious global health threat and a significant socio‐economic burden in many areas of the world. Almost all current clinical practice guidelines on the management of chronic hepatitis B (CHB) infection recommend that eligible patients pursue the optimal treatment endpoint, which is defined asHBsAg loss with or without anti‐HBs seroconversion. This review describes the effects of various regimens containing pegylated interferon (peg‐IFN)‐alpha on functional cure and the outcome of hepatocellular carcinoma (HCC) in patients withCHB. Peg‐IFN‐α monotherapy is a treatment option recommended by local and international clinical practice guidelines to help moreCHBpatients achieve a sustained off‐treatment virological response, which is particularly appropriate for relatively young patients who demand a finite treatment approach. Peg‐IFN‐α add‐on or sequential therapy in patients who have achieved a suppressed viral load after nucleos(t)ide analog (NA) therapy may offer further benefits onHBeAg seroconversion andHBsAg decline, although the effects of de novo combination therapy with peg‐IFN‐α andNAs on long‐term outcomes remain unclear. Evaluation of baseline and on‐treatment predictors is useful for selecting the patients who are likely to achieve additional benefits. Furthermore, some recent studies have shown that peg‐IFN‐α–based therapy results in better prevention ofHBV‐related hepatocellular carcinoma (HCC), especially in high‐risk patients.
Evaluation of a logistic regression model for predicting liver necroinflammation in hepatitis B e antigen‐negative chronic hepatitis B patients with normal and minimally increased alanine aminotransferase levels
Authors: Xie Yao , Yi Wei , Zhang Lu , Lu Yao , Hao Hong‐Xiao , Gao Yuan‐Jiao , Ran Chong‐Ping , Lu Hui‐Hui , Chen Qi‐Qi , Shen Ge , Wu Shu‐Ling , Chang Ming , Ping‐Hu Lei , Liu Rui‐Yu , Sun Lei , Wan Gang , Li Ming‐Hui 
Published: August 2019

AbstractLiver necroinflammation is the indicator for treating patients with chronic hepatitis B (CHB) infection. However, there is no suitable non‐invasive index for diagnosing liver necroinflammation. This study aimed to create a non‐invasive index to predict liver necroinflammation in patients who lack clear‐cut clinical inflammation parameters. Patients who were hepatitis B e antigen (HBeAg)‐negative and underwent liver histological diagnosis, had a normal or minimally increased alanine aminotransferase (ALT) level were enrolled. Liver necroinflammation was defined as histological active index ≥4. A logistic regression model (LRM) was established based on the parameters independently associated with liver necroinflammation. Of all 550 patients, 36.73% had necroinflammation. In patients with an abnormal ALT level, the rate of necroinflammation was 52.49%. The area under the curve (AUC) of the ALT level for predicting necroinflammation was 0.655 (95% confidence interval [CI], 0.609‐0.702), and that of the HBV DNA level ≥2000 IU/mL combined with an abnormal ALT level was 0.618. By using the LRM, the AUC improved to 0.769 (95% CI, 0.723‐0.815) with a Youden index of 0.519 and diagnostic accuracy of 75.3%. The cutoff value ≥0.7 in the LRM had a specificity of 97.4% and positive predictive value of 85.0% for predicting necroinflammation. By using the cutoff value <0.15 in the LRM, the presence of necroinflammation could be excluded with a negative predictive value of 90.8%. This study indicated that the LRM can be used to effectively diagnose liver necroinflammation in HBeAg‐negative patients with CHB who have normal or minimally elevated ALT levels.
The oak syngameon: more than the sum of its parts
Authors: Cannon Charles H. , Petit Rémy J. 
Published: August 2019

SummaryOne of Anthropocene's most daunting challenges for conservation biology is habitat extinction, caused by rapid global change. Tree diversity has persisted through previous episodes of rapid change, even global extinctions. Given the pace of current change, our management of extant diversity needs to facilitate and even enhance the natural ability of trees to adapt and diversify. Numerous processes contribute to this evolutionary flexibility, including introgression, a widespread yet under‐studied process. Reproductive networks, in which species remain distinct despite interspecific gene flow, are called syngameons, a concept largely inspired from work focusing on Quercus. Delineating and analyzing such species groups, empirically and theoretically, will provide insights into the nonadditive effects on evolution of numerous partially interfertile species exchanging genetic material episodically under changing environmental conditions. To conserve tree diversity, crossing experiments designed with an empirical and theoretical understanding of the constituent syngameon should be set up to assist diversification and adaptation in the Anthropocene. Our increasingly detailed knowledge of the oak genome and of oak interspecific and intraspecific phenotypic variation will improve our ability to sustain the diversity of this tree through an unpredictable and unprecedented future.
Nutrient supplementation during the first 1000 days and growth of infants born to pregnant adolescents
Authors: Dewey Kathryn G. , Matias Susana L. , Mridha Malay K. , Arnold Charles D. 
Published: August 2019

AbstractFew studies have evaluated the impact of nutritional supplementation among pregnant adolescents. We examined the effects of the Rang Din Nutrition Study (RDNS) interventions on children born to mothers <20 years of age. The RDNS was a cluster‐randomized effectiveness trial with four arms: (1) women and children both received small‐quantity lipid‐based nutrient supplements (LNS‐LNS), (2) women received iron and folic acid (IFA) and children received LNS (IFA‐LNS), (3) women received IFA and children received micronutrient powder (MNP) (IFA‐MNP), and (4) women received IFA and children received no supplements (IFA‐Control). We enrolled 4011 women at <20 weeks gestation; 1552 were adolescents. Among adolescents, prenatal LNS reduced newborn stunting by 25% and small head size by 28% and had a marginally significant effect on newborn wasting, compared with IFA. Low birth weight and preterm birth were reduced only among adolescents with lower food security. Effects on subsequent growth status were observed only among female children in the LNS‐LNS group: less stunting at 18 months (versus IFA‐MNP) and lower prevalence of small head circumference and wasting at 24 months (versus IFA‐Control). Initiatives targeting pregnant adolescents in similar settings should consider inclusion of small‐quantity LNS, particularly for adolescents living in food‐insecure households.
Epidemiology of the U.S. opioid crisis: the importance of the vector
Authors: Compton Wilson M. , Jones Christopher M. 
Published: August 2019

AbstractThe roots of the remarkably lethal U.S. opioid crisis are complex and inextricably entangled with healthcare, especially in its treatment of another serious health problem: pain. Failures of the healthcare system—including lack of both training in pain management and caution in using an addictive class of medications—precipitated the rise in opioid misuse and addiction over the past two decades, but a wider range of social and economic forces has helped perpetuate the crisis and altered its character. The classic epidemiologic host–agent–environment triad can be augmented for the purpose of elucidating the current opioid crisis by addition of the “vector” to emphasize the importance of purveyors of opioids (licit and illicit), leading to our proposing an expanded host–agent–vector–environment model. Interventions addressing multiple components are needed, including solutions that account for behaviors of all vectors associated with the crisis. For prescription opioids, the vectors include clinicians and pharmaceutical‐related companies involved in marketing, prescribing, distributing, and dispensing opioid medications; for illicit opioids, they include drug manufacturing and distribution networks. Attending to the vectors of opioids, while simultaneously implementing a full range of public health, clinical, law enforcement, and other approaches to ending the opioid crisis, may help to improve public health outcomes.
Diversity of virulence phenotypes among annual populations of Puccinia triticina originating from common wheat in Israel during the period 2000–15
Authors: Kosman E. , Ben‐Yehuda P. , Manisterski J. , Sela H. 
Published: August 2019

Leaf rust, caused by the fungus Puccinia triticina, is the most common rust disease of wheat in wheat‐producing areas worldwide. The Israeli population of P. triticina has been consistently monitored since 1993. A total of 784 single urediniospore isolates from Triticum aestivum were analysed during 2000–15. The structure of the pathogen population has changed to a large extent since 2000. The annual populations of P. triticina were separated into two distinct groups, 2000–11 and 2012–15, while populations of 2000–5 and 2006–12 were differentiated to a lesser extent. The change in the population originating from T. aestivum during the period 2000–15 is less significant compared to changes in the 1990s described previously. Diversity within the annual populations of P. triticina was rather stable during the period studied. Three new pathotypes, characterized by virulences on Lr3ka and Lr30 genes, became dominant between 2012 and 2015, while all but one prevailing pathotypes in 2000–11 were avirulent on these two genes. Significant changes in virulence frequencies on a number of Lr genes (Lr2c, Lr3ka, Lr15, Lr21, Lr23, Lr26, Lr30) and pairwise associations of virulences (mainly with Lr2c and Lr26) were registered in 2012–15 or earlier. It is postulated that the composition and pathotype structure of the P. triticina population in Israel is determined by wind‐disseminated urediniospores from neighbouring regions, where the migration of P. triticina from the eastern part of the Mediterranean Sea and from the Horn of Africa seem to have the greatest influence.
The Coming of Age of Risk Governance
Authors: Klinke Andreas , Renn Ortwin 
Published: August 2019

AbstractProposed as an advanced conceptualization of how to handle risk, risk governance begins with the critique and expansion of the traditional idea and standard practices of risk analysis. In developments over the last two decades, proponents of a more integrative approach on governing risks have moved further away from distinct conceptions of risk assessment, risk management, and risk communication and toward the processes and institutions that guide, restrain, and integrate collective activities of handling risk. In early formulations of what risk governance entails, the superiority of the interplay between risk evaluation and risk management over linear and simple deductions from risk assessment to risk management was established precisely by developing a distinctive rationality of how to proceed. Later, the International Risk Governance Council recaptured this distinctive rationality that institutionalized processes should embody the interplay of the assessment of risks and related concerns, their sociopolitical appraisal, and the logical inference for risk management. Recently, this approach has been refined and augmented toward an integrative and adaptive concept of risk governance and toward a postnormal conception of risk governance. Main characteristics are a new concept of differentiated responsibility and deliberation in which expertise, experience, and tacit knowledge are integrated, forming the core of legitimate political risk decision making.
Indices of Non-Ignorable Selection Bias for Proportions Estimated from Non-Probability Samples
Authors: Andridge Rebecca R. , West Brady T. , Little Roderick J. A. , Boonstra Philip S. , Alvarado-Leiton Fernanda 
Published: August 2019

SummaryRising costs of survey data collection and declining response rates have caused researchers to turn to non-probability samples to make descriptive statements about populations. However, unlike probability samples, non-probability samples may produce severely biased descriptive estimates due to selection bias. The paper develops and evaluates a simple model-based index of the potential selection bias in estimates of population proportions due to non-ignorable selection mechanisms. The index depends on an inestimable parameter ranging from 0 to 1 that captures the amount of deviation from selection at random and is thus well suited to a sensitivity analysis. We describe modified maximum likelihood and Bayesian estimation approaches and provide new and easy-to-use R functions for their implementation. We use simulation studies to evaluate the ability of the proposed index to reflect selection bias in non-probability samples and show how the index outperforms a previously proposed index that relies on an underlying normality assumption. We demonstrate the use of the index in practice with real data from the National Survey of Family Growth.
Decoding inflammation, its causes, genomic responses, and emerging countermeasures
Authors: Hawiger Jacek , Zienkiewicz Jozef 
Published: August 2019

AbstractInflammation is the mechanism of diseases caused by microbial, autoimmune, allergic, metabolic and physical insults that produce distinct types of inflammatory responses. This aetiologic view of inflammation informs its classification based on a cause‐dependent mechanism as well as a cause‐directed therapy and prevention. The genomic era ushered in a new understanding of inflammation by highlighting the cell's nucleus as the centre of the inflammatory response. Exogenous or endogenous inflammatory insults evoke genomic responses in immune and non‐immune cells. These genomic responses depend on transcription factors, which switch on and off a myriad of inflammatory genes through their regulatory networks. We discuss the transcriptional paradigm of inflammation based on denying transcription factors’ access to the nucleus. We present two approaches that control proinflammatory signalling to the nucleus. The first approach constitutes a novel intracellular protein therapy with bioengineered physiologic suppressors of cytokine signalling. The second approach entails control of proinflammatory transcriptional cascades by targeting nuclear transport with a cell‐penetrating peptide that inhibits the expression of 23 out of the 26 mediators of inflammation along with the nine genes required for metabolic responses. We compare these emerging anti‐inflammatory countermeasures to current therapies. The transcriptional paradigm of inflammation offers nucleocentric strategies for microbial, autoimmune, metabolic, physical and other types of inflammation afflicting millions of people worldwide.
Communicating and Disagreeing with Distinct Concepts: A Defense of Semantic Internalism
Authors: Valente Matheus 
Published: August 2019

AbstractI suggest a solution to a conflict between semantic internalism – according to which the concepts one expresses are determined by one's use of representations – and publicity – according to which, if two subjects successfully communicate or are in genuine agreement, then they entertain thoughts constituted by the same concepts. My solution rests on the thesis that there can be successful communication and genuine agreement between thinkers employing distinct concepts as long as there is a certain relation (of conceptually guaranteed sameness of extension) between them. In section 2, I motivate semantic internalism and show how it conflicts with publicity. In section 3, I carve the logical space of possible solutions to the conflict into liberal and conservative solutions. Section 4 assesses Wikforss's conservative solution to Burge's arthritis thought‐experiment and concludes that it fails for more than one reason. Section 5 introduces a new case study involving a deferential concept. This case serves as the backdrop for my positive account offered in section 6. The conclusion of the article is preceded by a comparison of my view with another recently proposed by Recanati (section 7) and some replies to possible objections (section 8).
The interaction between RUNX2 and core binding factor beta as a potential therapeutic target in canine osteosarcoma
Authors: Alegre Fernando , Ormonde Amanda R. , Godinez Dayn R. , Illendula Anuradha , Bushweller John H. , Wittenburg Luke A. 
Published: August 2019

AbstractOsteosarcoma remains the most common primary bone tumour in dogs with half of affected dogs unable to survive 1 year beyond diagnosis. New therapeutic options are needed to improve outcomes for this disease. Recent investigations into potential therapeutic targets have focused on cell surface molecules with little clear therapeutic benefit. Transcription factors and protein interactions represent underdeveloped areas of therapeutic drug development. We have utilized allosteric inhibitors of the core binding factor transcriptional complex, comprised of core binding factor beta (CBFβ) and RUNX2, in four canine osteosarcoma cell lines Active inhibitor compounds demonstrate anti‐tumour activities with concentrations demonstrated to be achievable in vivo while an inactive, structural analogue has no activity. We show that CBFβ inhibitors are capable of inducing apoptosis, inhibiting clonogenic cell growth, altering cell cycle progression and impeding migration and invasion in a cell line‐dependent manner. These effects coincide with a reduced interaction between RUNX2 and CBFβ and alterations in expression of RUNX2 target genes. We also show that addition of CBFβ inhibitors to the commonly used cytotoxic chemotherapeutic drugs doxorubicin and carboplatin leads to additive and/or synergistic anti‐proliferative effects in canine osteosarcoma cell lines. Taken together, we have identified the interaction between components of the core binding factor transcriptional complex, RUNX2 and CBFβ, as a potential novel therapeutic target in canine osteosarcoma and provide justification for further investigations into the anti‐tumour activities we describe here.