bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipopolysaccharide-Stimulated Human Fetal Membranes Induce Neutrophil Activation and Release of Vital Neutrophil Extracellular Traps
Authors: Tong Mancy , Potter Julie A. , Mor Gil , Abrahams Vikki M. 
Published: June 2019

AbstractPreterm birth is a major contributor to neonatal mortality and morbidity, and infection is a major risk factor. Chorioamnionitis, inflammation of the placenta, and fetal membranes (FMs) are commonly observed in preterm birth and are characterized by neutrophil infiltration. However, interactions between FMs and neutrophils remain incompletely understood. The objectives of this study were to determine how FMs, with or without bacterial LPS stimulation, affect neutrophil recruitment, activation, and the formation of neutrophil extracellular traps (NETs) and to elucidate the signaling mechanisms involved. Using a combination of in vitro, ex vivo, and in vivo approaches, we show that human resting FMs can directly recruit neutrophils and induce them to produce proinflammatory factors. Furthermore, neutrophils release vital NETs in response to FM-derived factors. LPS-stimulated FMs further augmented neutrophil recruitment, inflammatory cytokine/chemokine secretion, and vital NET release and also induced reactive oxygen species production and degranulation. We demonstrate a role for FM-derived TNF-α in mediating these effects through activation of neutrophil p38 MAPK. We propose that, during infection, neutrophil recruitment and activation may neutralize pathogens, vital NET formation, and prolonged neutrophil viability, and in combination with degranulation, reactive oxygen species production and inflammatory chemokine/cytokine production may contribute to tissue injury at the maternal/fetal interface.
Helios Deficiency Predisposes the Differentiation of CD4+Foxp3− T Cells into Peripherally Derived Regulatory T Cells
Authors: Skadow Mathias , Penna Vinay R. , Galant-Swafford Jessica , Shevach Ethan M. , Thornton Angela M. 
Published: June 2019

Abstract
The transcription factor Helios is expressed in a large percentage of Foxp3+ regulatory T (Treg) cells and is required for the maintenance of their suppressive phenotype, as mice with a selective deficiency of Helios in Treg cells spontaneously develop autoimmunity. However, mice with a deficiency of Helios in all T cells do not exhibit autoimmunity, despite the defect in the suppressor function of their Treg cell population, suggesting that Helios also functions in non-Treg cells. Although Helios is expressed in a small subset of CD4+Foxp3− and CD8+ T cells and its expression is upregulated upon T cell activation, its function in non-Treg cells remains unknown. To examine the function of Helios in CD4+Foxp3− T cells, we transferred Helios-sufficient or -deficient naive CD4+Foxp3− TCR transgenic T cells to normal recipients and examined their capacity to respond to their cognate Ag. Surprisingly, Helios-deficient CD4+ T cells expanded and differentiated into Th1 or Th2 cytokine-producing effectors in a manner similar to wild-type TCR transgenic CD4+ T cells. However, the primed Helios-deficient cells failed to expand upon secondary challenge with Ag. The tolerant state of the Helios-deficient memory T cells was not cell-intrinsic but was due to a small population of Helios-deficient naive T cells that had differentiated into Ag-specific peripheral Treg cells that suppressed the recall response in an Ag-specific manner. These findings demonstrate that Helios plays a role in the determination of CD4+ T cell fate.
Evaluation of genetic diversity of edible honeysuckle monitored by RAPD in relation to bioactive substances
Authors: Cehula Marcela , Jurí­ková Tünde , Žiarovská Jana , Mlček Jiří­ , Kyseľ Matúš 
Published: June 2019

The aim of this study was clarifying the relation between genetic diversity of edible honeysuckle (Lonicera kamtschatica) and the major group of biologically active substances as total polyphenols content (TPC) including antioxidant activity (AO). Fruits of edible honeysuckle becomes more and more popular, especially in Europe. The current status of research on polyphenolic compounds in the berries of edible honeysuckle and their biological effects, including recommended utilization, are reviewed.The biological material including 14 cultivars of the edible honeysuckle (´Zoluška´, ´Amfora´, ´Pruhonický 44´, ´Vasilijevsky´, ´Moskovskaja´, ´Vojtek´, ´Sinoglaska´, ´Altaj´, ´Lipnická´, ´Kamčadalka´, ´Sinaja Ptica´, ´Fialka´, ´Modrý Triumf´, and ´Leningradský velikán´) originated from Czech republic (Žabcice near Brno). The content of TPC and AO were determined by location and its soil-climatic conditions and these environmental circumstances determines the RAPD profiles of analysed honeysuckle acessions, too. DPPH method was used to analyze AO and Folin-Ciocalteu method was used to determine TPC. The results of experiment showed that the highest value of AO was determined at the cultivars ´Zoluška´ (81.04 mg.L-1) and the lowest was measured in ´Kamčadalka´ (54.122 mg.L-1). On the contrary, the highest content of TPC was determined at the cultivar ´Kamčadalka´ (51.09 mg.L-1) and the lowest value was measured at the cultivar ´Pruhonický 44´ (21.65 mg.L-1). Phylogenetic trees were similar in genetic distance. The content of TPC and AO were not statistically significant in relation to cultivar. The analyzed cultivars of the edible honeysuckle were separated in 4 clusters according to used primers. In both gel images, the amplicon size ranged from 100 to 1,500 bp. We found that genetic diversity was partially related to content of total polyphenolic substances and antioxidant activity. Based on phylogenetic trees we have stated that variety ´Lipnická´, ´Sinoglaska´, ´Altaj´, ´Leningradský velikán´, ´Modrý Triumf´, ´Sinaja Ptica´ and ´Kamčadalka´ were grouped in the similar cluster. The highest genetic distance was determined at the variety ´Vasilijevskaja´ and ´Amfora´. In the same way, there were variety ´Vojtek´, ´Fialka´ and ´Zoluška´.
Effects of Thioxanthene Containing Anti-Psychotic and Anti-Platelet Drug Combination on Mean Platelet Volume and Platelet Distribution Width in Rats
Authors: Bashir Muhammad Irfan , Saleem Uzma , Anwer Fareeha , Ahmad Bashir 
Published: June 2019

Aim: To evaluate the combined and individual effects of thioxanthene containing antipsychotic and anti-platlet drug on Mean Platelet Volume (MPV) and Platelet Distribution Width (PDW) in rats.
Methods: This investigational study comprised of 100 albino rats of both sexual orientation, they were of 300 g to 350 g. We got ten groups, in which each group consisted of 10 rats (n=10).Ozagrel was used as anti-platelet and Zuclopenthixol was used as thioxanthene containing antipsychotic. Rats were treated with defined doses of Ozagrel and thioxanthene containing anti-psychotic (Zuclopenthixol) alone and in joined for three weeks (21 days). Blood test at 0, seventh, fourteenth and last day of study were taken. Mean Platelet Volume and Platelet Distribution Width were estimated from blood tests by using standard research center procedure. Results were accumulated and abridged by applying statistics. Correlation was framed between all days incentive to zero day values.
Results: Anti-psychotic drug and antiplatelet drug both showed decrease in MPV with both doses highly significantly (p < 0.001) decrease associated with maximum doses alone and with combination group. In case of PDW all individual and minimum combination group showed increase in PDW values but significantly (p < 0.001) increase in maximum combination groups.
Conclusion: Combination of both drugs can cause more decrease in MPV as compare to the individual and in case of PDW combination with maximum doses may cause decrease in values by opposing the results individual therapy. It indicated any Drug-Drug interaction between these two drugs regarding Mean platelet volume and Platelet distribution width.
Retracted: Consistency analysis of microRNA‐arm expression reveals microRNA‐369‐5p/3p as tumor suppressors in gastric cancer
Authors: Dong Lei , Zhang Zhengyi , Xu Jiayue , Wang Fang , Ma Yanni , Li Feng , Shen Chao , Liu Ziwen , Zhang Junwu , Liu Changzheng , Yi Ping , Yu Jia 
Published: June 2019

The 5p and 3p arms of microRNA (miRNA) are typically generated from the same precursor, and one arm influences protein output, while the other has a short half‐life. However, a few miR‐5p/3p pairs have been reported to co‐exist in cancer cells. Here, we performed a genome‐wide analysis of miRNA expression in gastric cancer (GC) cells to systematically investigate the co‐expression profile of miR‐5p/3p in gastric tumorigenesis. We discovered that only 41 miR‐5p/3p pairs out of 1749 analyzed miRNA were co‐expressed. Specifically, abnormal expression of miR‐369‐5p and miR‐369‐3p was correlated with GC progression. Importantly, both in vitro and in vivo assays revealed that miR‐369‐5p and miR‐369‐3p exhibited tumor‐suppressive roles by regulating jun proto‐oncogene and v‐akt murine thymoma viral oncogene homolog 1 function in GC cells, respectively. Moreover, we observed that miR‐369 was inactivated in GC tissues due to DNA methylation. We also showed that inhibition of miR‐369‐5p/3p attenuated the effect of azacitidine (AZA) treatment on suppressing cell growth and invasion. These results suggest that the therapeutic efficacy of AZA in GC is at least partly attributable to miR‐369 activation. Overall, our findings provide convincing evidence that both the 5p and 3p arms of miRNA co‐expressed in GC and DNA methylation‐induced miR‐369 signaling contribute to GC progression.
Magnetically Addressable Shape‐Memory and Stiffening in a Composite Elastomer
Authors: Testa Paolo , Style Robert W. , Cui Jizhai , Donnelly Claire , Borisova Elena , Derlet Peter M. , Dufresne Eric R. , Heyderman Laura J. 
Published: June 2019

AbstractWith a specific stimulus, shape‐memory materials can assume a temporary shape and subsequently recover their original shape, a functionality that renders them relevant for applications in fields such as biomedicine, aerospace, and wearable electronics. Shape‐memory in polymers and composites is usually achieved by exploiting a thermal transition to program a temporary shape and subsequently recover the original shape. This may be problematic for heat‐sensitive environments, and when rapid and uniform heating is required. In this work, a soft magnetic shape‐memory composite is produced by encasing liquid droplets of magneto‐rheological fluid into a poly(dimethylsiloxane) matrix. Under the influence of a magnetic field, this material undergoes an exceptional stiffening transition, with an almost 30‐fold increase in shear modulus. Exploiting this transition, fast and fully reversible magnetic shape‐memory is demonstrated in three ways, by embossing, by simple shear, and by unconstrained 3D deformation. Using advanced synchrotron X‐ray tomography techniques, the internal structure of the material is revealed, which can be correlated with the composite stiffening and shape‐memory mechanism. This material concept, based on a simple emulsion process, can be extended to different fluids and elastomers, and can be manufactured with a wide range of methods.
Organic Electrochemical Transistors: Smart Devices for Real‐Time Monitoring of Cellular Vitality
Authors: Decataldo Francesco , Barbalinardo Marianna , Tessarolo Marta , Vurro Vito , Calienni Maria , Gentili Denis , Valle Francesco , Cavallini Massimiliano , Fraboni Beatrice 
Published: June 2019

AbstractCurrent technologies to monitor formation and disruption in in vitro cell cultures are based either on optical techniques or on electrical impedance/resistance measurement, which often rely on cumbersome and time‐consuming measurements and data analyses. In this paper, poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS)‐based organic electrochemical transistors (OECTs) are proposed with channel areas specifically designed and dimensioned as fast and real‐time monitoring devices for a large variety of cell lines, with a broad range of tissue resistance. In particular, it is investigated how and why two device configurations provide a different response to leaky‐barrier (NIH‐3T3) and strong‐barrier (CaCo‐2) cell lines growth and detachment, achieving a continuous monitoring also for leaky‐barrier cell layer growth and detachment. Data are collected using the transistor dynamic behavior to a DC potential pulse on the gate, providing an excellent time resolution and thus enhancing the amount of information that can be collected for fast biological processes (<5 s).
Bismutverbindungen in der Radikalkatalyse: Übergangsmetallbismutane ermöglichen thermisch induzierte Cycloisomerisierungen
Authors: Ramler Jacqueline , Krummenacher Ivo , Lichtenberg Crispin 
Published: June 2019

AbstractDer Einsatz von Bismutverbindungen in kontrollierten Radikalreaktionen steckt noch in den Kinderschuhen. Neue Entwicklungen werden durch die Eigenschaften dieser Komplexe vorangetrieben, die für Anwendungen in der Synthesechemie hochattraktiv sind (z. B. niedrige Toxizität, hohe Toleranz gegenüber funktionellen Gruppen, niedrige homolytische Bindungsdissoziationsenergien und reversible homolytische Bindungsdissoziationen). Hier berichten wir über die erste katalytische Anwendung von Übergangsmetallbismutanen (d. h. Verbindungen mit einer Bi‐ÜM‐Bindung; ÜM=Übergangsmetall). In der katalysierten radikalischen Cycloisomerisierung von δ‐Iodolefinen als Modellreaktion werden Eigenschaften sichtbar, die im Vergleich zu bekannten B‐, Mn‐, Cu‐, Zn‐, Sn‐ und Alkalimetallreagenzien komplementär oder überlegen sind (einschließlich eines anderen entscheidenden Zwischenprodukts). Somit werden Übergangsmetallbismutane als neue Klasse von (Prä‐)Katalysatoren für kontrollierte Radikalreaktionen eingeführt.
Carbon Dots as a New Class of Diamagnetic Chemical Exchange Saturation Transfer (diaCEST) MRI Contrast Agents
Authors: Zhang Jia , Yuan Yue , Gao Minling , Han Zheng , Chu Chengyan , Li Yuguo , van Zijl Peter C. M. , Ying Mingyao , Bulte Jeff W. M. , Liu Guanshu 
Published: June 2019

AbstractWhile carbon dots (C‐dots) have been extensively investigated pertaining to their fluorescent, phosphorescent, electrochemiluminescent, optoelectronic, and catalytic features, their inherent chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) properties are unknown. By virtue of their hydrophilicity and abundant exchangeable protons of hydroxyl, amine, and amide anchored on the surface, we report here that C‐dots can be adapted as effective diamagnetic CEST (diaCEST) MRI contrast agents. As a proof‐of‐concept demonstration, human glioma cells were labeled with liposomes with or without encapsulated C‐dots and implanted in mouse brain. In vivo CEST MRI was able to clearly differentiate labeled cells from non‐labeled cells. The present findings may encourage new applications of C‐dots for in vivo imaging in deep tissues, which is currently not possible using conventional fluorescent (near‐infrared) C‐dots.
Fulminating Gold and Silver
Authors: Wentrup Curt 
Published: June 2019

AbstractThis Essay deals with the fascinating and highly explosive compounds fulminating gold and fulminating silver, which are easily made by treatment of gold dissolved in aqua regia with ammonia, and by reaction of silver oxide or silver salts with ammonia, respectively. Fulminating gold in particular captivated the alchemists in the 16th to 18th centuries. Numerous preparations were described, as well as numerous attempts to make volatile, sublimable or distillable gold, and to use the products so obtained (which were most likely gold chlorides) to make the sought‐after tincture, which would “heal” the “impure” metals and transform them into gold, and equally be a panacea to cure all human illnesses.
Synergistic effect between zeolitic imidazolate framework‐8 and expandable graphite to improve the flame retardancy and smoke suppression of polyurethane elastomer
Authors: Wang Guisong , Xu Wenzong , Chen Rui , Li Wu , Liu Yucheng , Yang Kai 
Published: June 2019

ABSTRACTZeolitic imidazolate framework‐8 (ZIF‐8) was synthesized at room temperature, and then added with expandable graphite (EG) to polyurethane elastomer (PUE) to study their flame retardancy and smoke suppression of PUE. The results showed that when the total flame retardant was 3 wt % and the ratio of ZIF‐8 and EG was 1:3, it had the best effect on the flame retardancy and smoke suppression of PUE. Compared with pure PUE, the peak heat release rate, the total heat release, and the maximum smoke density values of Z1E3 decreased by 83.4, 42.6, and 22.4%, respectively. Moreover, the limited oxygen index value of Z1E3 increased to 30.2% and its UL‐94 vertical burning test reached V‐1 rating. This was because ZIF‐8 synergized with EG to make the intumescent char layer compact, resulting in improved flame retardancy and smoke suppression of PUE. The specific mechanism of flame retardancy and smoke suppression is also discussed in this article. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48048.
Conducting Comprehensive Assessment Within Community Colleges: Administrative, Educational, and Student Support (AES) Assessment with the Shults Dorimé‐Williams Taxonomy
Authors: Dorimé‐Williams Marjorie L. , Shults Christopher 
Published: June 2019

AbstractThis chapter introduces the Borough of Manhattan Community College's Institutional Effectiveness Assessment Plan and the Shults Dorimé‐Williams Taxonomy as resources for the enhancement of assessment within administrative, educational, and student support units.
Polymers of Intrinsic Microporosity in Triphasic Electrochemistry: Perspectives
Authors: Marken Frank , Madrid Elena , Zhao Yuanzhu , Carta Mariolino , McKeown Neil B. 
Published: June 2019

AbstractPolymers of intrinsic microporosity (PIMs) as molecularly rigid polymers have emerged as a new class of gas‐permeable glassy materials. They offer excellent processability and a range of potential applications also in electrochemical processes. Particularly interesting is the ability of some PIM films to remain gas‐permeable/binding even in the presence of (aqueous) liquid electrolyte to give triphasic interfacial reactivity. Gaseous reagents or products (such as hydrogen or oxygen) are bound probably into hydrophobic regions in the wet PIM film to avoid macroscopic bubble formation and to enhance both the surface reactivity and the apparent activity of the gas solute close to the electrode/catalyst surface. The photoelectrochemical formation of hydrogen gas close to a platinum electrode is enhanced by PIM‐1, which is presented as an example of energy harvesting through molecular H2 “energy carrier” transport.
Survey on the Mechanical Properties of Lamellar Ag‐MXA Supercluster Architectures
Authors: Qin Xiaoyun , Li Ailin , Liu Keyan , Xue Zhenjie , Song Qian , Qin Xiaomei , Wang Tie 
Published: June 2019

AbstractLamellar nanomaterials with specific architectures and novel properties have received increasing attention from both scientific and technological fields in recent years because of their potential applications in catalysis, energy conversion, and storage devices. Bulk supercluster pellets with well‐defined lamellar structures were fabricated by assembling silver clusters and mercaptoalkyl acids (MXA) to investigate the mechanical properties. The relationship between the assembled structure and pressure resistance was surveyed for the first time. The enhanced interlayer interactions were found to increase the elastic modulus of the Ag‐MXA supercluster architectures.
Recent Contributions to the Coordination Chemistry of Beryllium
Authors: Buchner Magnus R. 
Published: June 2019

AbstractGiven the alleged extreme toxicity of beryllium and its compounds, it is the least investigated nonradioactive element. However, beryllium exhibits unique properties among the elements and therefore the related coordination chemistry is unprecedented. With the emergence of s‐block catalysis and the introduction of low‐valent magnesium complexes as versatile reducing agents, the interest in related beryllium compounds also has increased. Therefore, some quite remarkable progress on the coordination and organometallic chemistry of beryllium has been made in the last two decades. This Minireview article gives an oversight over the relatively recent developments in this field of beryllium research. An emphasis is placed on the correlation between the structure and reactivity of the described compounds.
Mechanistic Insights into Statistical Co‐Assembly of Metal Complexes
Authors: Coelho Joao Paulo , Matern Jonas , Albuquerque Rodrigo Q. , Fernández Gustavo 
Published: June 2019

AbstractInvited for the cover of this issue is the group of Gustavo Fernández at the Westfälische Wilhelms‐Universität Münster. The image depicts the two‐step cooperative cooling curve obtained during the co‐assembly process of the equimolar mixture of the target PdII and PtII complexes superimposed on the Sugarloaf mountain from one of the most photographed spots in Brazil. Read the full text of the article at 10.1002/chem.201900604.
Automated reduction of blood coagulation models
Authors: Hansen Kirk B. , Shadden Shawn C. 
Published: June 2019

AbstractMathematical modeling of thrombosis typically involves modeling the coagulation cascade. Models of coagulation generally involve the reaction kinetics for dozens of proteins. The resulting system of equations is difficult to parameterize, and its numerical solution is challenging when coupled to blood flow or other physics important to clotting. Prior research suggests that essential aspects of coagulation may be reproduced by simpler models. This evidence motivates a systematic approach to model reduction. We herein introduce an automated framework to generate reduced‐order models of blood coagulation. The framework consists of nested optimizations, where an outer optimization selects the optimal species for the reduced‐order model and an inner optimization selects the optimal reaction rates for the new coagulation network. The framework was tested on an established 34‐species coagulation model to rigorously consider what level of model fidelity is necessary to capture essential coagulation dynamics. The results indicate that a nine‐species reduced‐order model is sufficient to reproduce the thrombin dynamics of the benchmark 34‐species model for a range of tissue factor concentrations, including those not included in the optimization process. Further model reduction begins to compromise the ability to capture the thrombin generation process. The framework proposed herein enables automated development of reduced‐order models of coagulation that maintain essential dynamics used to model thrombosis.
Polystyrene with Persistently Enhanced Fluorescence: Photo‐Induced Atom Transfer Radical Polymerization Using a Pyrene‐Based Initiator
Authors: Su Chun‐Hsien , Wu Zhenqiang , Lin Ching‐Kai , Han Hui‐An , Chen Yi‐An , Chou Pi‐Tai , Fu Xuefeng , Peng Chi‐How 
Published: June 2019

AbstractThe pyrene‐based atom transfer radical polymerization (ATRP) initiator of 1‐pyrenemethyl 2‐bromoisobutyrate (PMBriB) was applied to the photo‐induced radical polymerization of methyl acrylate, methyl methacrylate, and styrene. During the polymerization, the fluorescence of the polymer solution caused by the chain end pyrene group was observed to be slightly enhanced in all cases, possibly due to the decline of the aggregation caused quenching (ACQ) effect. However, the fluorescence of polystyrene obtained from photo‐induced ATRP was significantly increased. Differential scanning calorimeter (DSC) measurements, NMR spectroscopy, and fluorescence measurements associated with a series of control studies demonstrated that the enhanced fluorescence was a persistent phenomenon in both solution and the solid state but the polystyrenes with and without enhanced fluorescence showed no observable difference in chemical bonding and molecular conformation. At this stage, the coexistence of pyrene, 2‐bromoisobutyrate group, styrene, and light was required for this novel phenomenon and the mechanism of this fluorescence enhancement is still being studied.