bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deoxynivalenol as potential modulator of human steroidogenesis
Authors: Urbanek Kinga Anna , Habrowska‐Górczyńska Dominika Ewa , Kowalska Karolina , Stańczyk Anna , Domińska Kamila , Piastowska‐Ciesielska Agnieszka Wanda 
Published: April 2018

AbstractDeoxynivalenol (DON) is a type B trichothecene, produced by the Fusarium species. Exposure to DON might cause disruptive effects such as reduced weight gain, neuroendocrine changes and immune modulation in animals (rats, dogs, pigs). There is huge concern that similar effects can be observed in humans. DON is a potential regulator of intracellular steroidogenesis. It is also possible that DON will be involved in the regulation of miRNAs connected with steroidogenesis. This review summarizes the latest knowledge about the influence of DON on steroidogenesis and human hormonal balance.
Is primary sidedness a prognostic factor in patients with resected colon cancer liver metastases (CLM)?
Authors: Marques Márcio C , C. Ribeiro Héber S. , Costa Wílson L. , de Jesus Victor Hugo F. , de Macedo Mariana P. , Diniz Alessandro L. , Godoy André L. , Farias Igor C. , Aguiar Samuel , Riechelmann Rachel S. P. , S. Begnami Maria Dirlei F. , Coimbra Felipe J. F. 
Published: April 2018

Background and ObjectivesRecent studies have suggested that sidedness of origin from colorectal adenocarcinomas is a predictor of survival, however the impact of this factor in patients with resected colon cancer liver metastases (CLM) is not clear. So, in this study, we compared clinic and pathologic characteristics and long‐term survival of patients with resected CLM according to the primary tumor location.MethodsThis is a retrospective analyzes of a prospective database. Patients with resected CLM from 1998 to 2012 were included. Right colon included tumors from cecum to middle transverse colon, and left colon included tumors from splenic flexure to sigmoid.ResultsOne hundred fifty‐one patients were included, 27 right colon and 124 left colon. In the latter group, there were more patients with synchronous disease (67.7 × 6.2%, P = 0.026) and a higher CEA (22.0 × 11.7 ng/mL, P = 0.001). However, K‐Ras mutation was more frequent in right sided tumors (75.0 × 24.1%, P = 0.001). There was no difference in long term survival in both groups in this series even when adjusted for the confounding variables.ConclusionSidedness do not seem to be a predictor of long term survival in patients with resected colon cancer liver metastases.
Applications of Livingston‐type inequalities to the generalized Zalcman functional
Authors: Efraimidis Iason , Vukotić Dragan 
Published: April 2018

AbstractWe obtain sharp estimates for a generalized Zalcman coefficient functional with a complex parameter for the Hurwitz class and the Noshiro–Warschawski class of univalent functions as well as for the closed convex hulls of the convex and starlike functions by using an inequality from [6]. In particular, we generalize an inequality proved by Ma for starlike functions and answer a question from his paper [17]. Finally, we prove an asymptotic version of the generalized Zalcman conjecture for univalent functions and discuss various related or equivalent statements which may shed further light on the problem.
Probing Chemical and Mechanical Nanodomains in Copolymer Nanorods with Correlative Atomic Force Microscopy—Nano‐correscopy
Authors: Khanal Dipesh , Zhang Bokai , Ramzan Iqbal , Marcott Curtis , Li Quan , Chrzanowski Wojciech 
Published: April 2018

AbstractThe interplay between size, shape, mechanical properties, and surface chemistry of nanoparticles orchestrates cellular internalization, toxicity, circulation time, and biodistribution. Therefore, the safety of nanoparticles hinges on our ability to quantify nanoscale physicochemical characteristics. Current characterization tools, due to their limited resolution, are unable to map these properties correlatively at nanoscale. An innovative use of atomic force microscopy‐based techniques, namely nano‐correscopy, overcomes this limitation and offers multiprobe capability to map mechanical (viscous and elastic) and chemical domains of nanoparticles correlatively. The strengths of this approach are demonstrated using polymer composite nanorods: m‐PEG‐PLGA ((m‐PEG–methoxy‐poly (ethylene glycol)‐b‐poly (lactic‐co‐glycolic) acid). Precise distribution of PLGA (monomers of lactide and glycolide) and poly(ethylene glycol) (PEG) polymer across nanorods is identified. The hydrophobic lactide component is found predominantly at the apex, while hydrophilic glycolide and PEG assembled at the body of the nanorods and correlate with a gradient of nanomechanical properties. New knowledge of how both nanochemical domains and nanomechanical properties are distributed across the nanorod will allow elucidating the interactions of nanorods with the proteins and biomolecules in the future, which will directly influence the fate of nanorods in vivo and will guide new synthesis methods.
Simple structural elucidation of ostreocin‐B, a new palytoxin congener isolated from the marine dinoflagellate Ostreopsis siamensis, using complementary positive and negative ion liquid chromatography/quadrupole time‐of‐flight mass spectrometry
Authors: Terajima Takehito , Uchida Hideaki , Abe Naoki , Yasumoto Takeshi 
Published: April 2018

RationaleOstreocin‐B is a new palytoxin congener of comparable complexity. Elucidation of the chemical structure by nuclear magnetic resonance has been hampered due to limited sample availability. More importantly, the dihedral angles of protons at the base of the hydroxyl groups on the ring structures are predicted to produce little couplings and thus disrupt connectivity. The present investigation solved the problem through mass spectrometry.MethodsStructural elucidation was performed by high‐performance liquid chromatograph coupled to a quadrupole time‐of‐flight mass spectrometer equipped with an electrospray ionization source operated in positive and negative ion mode. Measurement parameters were optimized to achieve high sensitivity and a high ratio of singly charged ions. Ostreocin‐D (C127H219N3O53), another palytoxin congener possessing an unambiguously determined structure, was used as a template.ResultsThe molecular formula of ostreocin‐B, C127H219N3O54, indicates that it has one more oxygen atom than ostreocin‐D. Comparison of the product ion spectra in negative ion mode indicates the occurrence of hydroxyl substitution at C44 in ostreocin‐B, unlike in ostreocin‐D. Positive ion spectra also support the 44‐OH substructure by producing conjugated polyenes ascribable to the sequential loss of hydroxyls on the cyclic hemiacetal.ConclusionsThe planar structure of ostreocin‐B is assigned to 42‐hydroxy‐3,26‐didemethyl‐19‐deoxypalytoxin (=44‐hydroxyostreocin‐D). The method used in this study is an excellent tool to obtain structural information on interspecies and intrastrain variation of palytoxin congeners in marine organisms.
On the use of the not‐at‐random fully conditional specification (NARFCS) procedure in practice
Authors: Tompsett Daniel Mark , Leacy Finbarr , Moreno‐Betancur Margarita , Heron Jon , White Ian R. 
Published: April 2018

The not‐at‐random fully conditional specification (NARFCS) procedure provides a flexible means for the imputation of multivariable missing data under missing‐not‐at‐random conditions. Recent work has outlined difficulties with eliciting the sensitivity parameters of the procedure from expert opinion due to their conditional nature. Failure to adequately account for this conditioning will generate imputations that are inconsistent with the assumptions of the user.In this paper, we clarify the importance of correct conditioning of NARFCS sensitivity parameters and develop procedures to calibrate these sensitivity parameters by relating them to more easily elicited quantities, in particular, the sensitivity parameters from simpler pattern mixture models. Additionally, we consider how to include the missingness indicators as part of the imputation models of NARFCS, recommending including all of them in each model as default practice.Algorithms are developed to perform the calibration procedure and demonstrated on data from the Avon Longitudinal Study of Parents and Children, as well as with simulation studies.
Design and optimization of a reversed‐phase HPLC with diode array detection method for the determination of acemetacin and its toxic impurities using experimental design
Authors: Abdelgawad Mohamed A. , Owis Asmaa I. , Abdelwahab Nada S. 
Published: April 2018

AbstractA reversed‐phase high‐performance liquid chromatography with diode array detection method was developed with the aid of statistical approaches for the determination of acemetacin and its official impurities 4‐chlorobenzoic acid and indometacin. Two‐level full factorial design was used to optimize the used mobile phase where three chromatographic variables were studied, amount of acetonitrile, amount of H3PO4 acid, and mobile phase flow rate. The selected responses were analysis time and chromatographic resolution between adjacent peaks (peaks of indometacin and acemetacin). The impacts of the amount of acetonitrile and the mobile phase flow rate individually were significant on the analysis time (P = 0.0277 and 0.0424, respectively). Also the amount of acetonitrile had a meaningful effect on the resolution (P = 0.0085). Finally, chromatographic separation was performed on Zorbax® Eclipse C18 column using acetonitrile/water (containing 0.05% H3PO4; 55:45, v/v) at 1.5 mL/min as an optimized mobile phase using full factorial experimental design with UV detection at 230 nm. 4‐Chlorobenzoic acid appeared at 3.29 min, indometacin at 9.07 min and acemetacin at 10 min. The method fulfilled United States Pharmacopoeia validation standards. In addition, system suitability parameters were calculated, and the results were within acceptable criteria. Commercially available tablets were analyzed, and good recoveries were obtained. Statistical comparison of the method with the reported one showed no significant difference.
Stepwise “bridge‐to‐bridge” reduction of monoclonal antibodies and light chain detection: Case studies of tenatumomab and trastuzumab
Authors: Mazzoccanti Giulia , Pierri Giuseppe , Ciogli Alessia , Ismail Omar H. , Giorgi Fabrizio , De Santis Rita , Villani Claudio , Gasparrini Francesco 
Published: April 2018

AbstractMonoclonal antibodies are pharmaceutical products that because of their biological origin are characterized by the presence of countless variants, which are generated by post‐translational modifications, during purification or storage. Among these modifications the disulfide bridges variants are of considerable importance, for example their status can affect the correct Y‐shape of antibody. An analytical method, to characterize the oxidized/reduced state of disulfide bridges and to monitor the formation of free light chains followed by chemical reduction, has been developed and here presented. The chromatographic set‐up employs a macroporous polystyrene‐divinylbenzene stationary phase in ion‐pair reversed‐phase high‐performance liquid chromatography and a double UV/high‐resolution mass spectrometry detector. The appearance of increasing amount of reduced light chains can be observed upon incubation of antibodies with the reducing agent tris(2‐carboxyethyl)phosphine. Specifically, three isoforms can be identified at different reaction times: light chain, partially reduced light chain+2H, with one of two disulfide bridges opened in the constant or variable region, and totally reduced light chain+4H. The method was applied in the case of two monoclonal antibodies, namely, tenatumomab and trastuzumab, resulting in a potential tool to detection disulfide bridges variants of other biopharmaceutical products during drug development and release.
Identifying dementia cases with routinely collected health data: A systematic review
Authors: Wilkinson Tim , Ly Amanda , Schnier Christian , Rannikmäe Kristiina , Bush Kathryn , Brayne Carol , Quinn Terence J. , Sudlow Cathie L.M. ,   On behalf of the UK Biobank Neurodegenerative Outcomes Group and Dementias Platform UK
Published: April 2018

AbstractIntroductionProspective, population‐based studies can be rich resources for dementia research. Follow‐up in many such studies is through linkage to routinely collected, coded health‐care data sets. We evaluated the accuracy of these data sets for dementia case identification.MethodsWe systematically reviewed the literature for studies comparing dementia coding in routinely collected data sets to any expert‐led reference standard. We recorded study characteristics and two accuracy measures—positive predictive value (PPV) and sensitivity.ResultsWe identified 27 eligible studies with 25 estimating PPV and eight estimating sensitivity. Study settings and methods varied widely. For all‐cause dementia, PPVs ranged from 33%–100%, but 16/27 were >75%. Sensitivities ranged from 21% to 86%. PPVs for Alzheimer's disease (range 57%–100%) were generally higher than those for vascular dementia (range 19%–91%).DiscussionLinkage to routine health‐care data can achieve a high PPV and reasonable sensitivity in certain settings. Given the heterogeneity in accuracy estimates, cohorts should ideally conduct their own setting‐specific validation.
Post‐tonsillectomy taste dysfunction: Myth or reality?
Authors: Soldatova Liuba , Doty Richard L. 
Published: April 2018

Lingual branches of the glossopharyngeal nerve (CN Ⅸ) are at risk of injury during tonsillectomy due to their proximity to the muscle layer of the palatine tonsillar bed. However, it is unclear how often this common surgery leads to taste disturbances. We conducted a literature search using PubMed, Embase, Cochrane Library, Google Scholar, PsychInfo, and Ovid Medline to evaluate the available literature on post‐tonsillectomy taste disorders. Studies denoting self‐reported dysfunction, as well as those employing quantitative testing, i.e., chemogustometry and electrogustometry, were identified. Case reports were excluded. Of the 8 original articles that met our inclusion criteria, only 5 employed quantitative taste tests. The highest prevalence of self‐reported taste disturbances occurred two weeks after surgery (32%). Two studies reported post‐operative chemical gustometry scores consistent with hypogeusia. However, in the two studies that compared pre‐ and post‐tonsillectomy test scores, one found no difference and the other found a significant difference only for the left rear of the tongue 14 days post‐op. In the two studies that employed electrogustometry, elevated post‐operative thresholds were noted, although only one compared pre‐ and post‐operative thresholds. This study found no significant differences. No study employed a normal control group to assess the influences of repeated testing on the sensory measures. Overall, this review indicates that studies on post‐tonsillectomy taste disorders are limited and ambiguous. Future research employing appropriate control groups and taste testing procedures are needed to define the prevalence, duration, and nature of post‐tonsillectomy taste disorders.
On properly characterizing moral agency
Authors: Fowers Blaine J. , Anderson Austen R. , Lang Samantha F. 
Published: April 2018

AbstractDoris (2015b) develops a theory of moral agency to avoid a skeptical challenge arising from psychology studies indicating that (im)moral behavior is caused by trivial situational factors. His theory is flawed in attending only to situational influences on behavior and neglecting individual differences such as moral identity and virtue. A focus on individual differences in resilience to influence from trivial situational factors defangs the skeptical challenge and offers a better account of moral agency.
Learning to talk to ourselves: Development, ignorance, and agency
Authors: Hammond Stuart I. 
Published: April 2018

AbstractAlthough anti-reflectivism seems to preclude a role for reflection, this dichotomy could be synthesized in a Piagetian developmental framework. Development integrates a role for error and ignorance in reflection, and supports Doris's espousal of valuation, collaboration, and pluralism, and the importance of extrinsic factors to the self.
Talking to others: The importance of responsibility attributions by observers
Authors: Hechler Stefanie , Kessler Thomas 
Published: April 2018

AbstractThis commentary extends Doris's approach of agency by highlighting the importance of responsibility attributions by observers. We argue that (a) social groups determine which standards are relevant and which actors are responsible, (b) consensus about these attributions may correct individual defeaters, and (c) the attribution of moral responsibility reveals agency of observers and may foster the actors' agency.
Talking to others' selves: Why a valuational paradigm of agency fails to provide an adequate theoretical framework for moral responsibility, social accountability, and legal liability
Authors: Mattei Tobias A. 
Published: April 2018

AbstractIn this commentary, I highlight the importance of a proper discussion of the pragmatic implications of John Doris's paradigm for allocation of personal responsibility proposed in his new book Talking to Our Selves. By employing some classic concepts of the American common law tradition, I discuss why Doris's valuational understanding of agency fails to provide an adequate framework for moral responsibility, social accountability, and legal liability.
High loss factor piezoelectric damping composite with three-dimensional reduced graphene oxide as the conductive phase
Authors: Xue Wenchao , Li Hua , Dugnani Roberto , Rehman Hafeez Ur , Zhang Chunmei , Chen Yujie , Liu Hezhou 
Published: April 2018

Piezoelectric damping composite with 3D rGO as the conductive phase that possesses good damping properties with the conductive threshold as low as 0.7 wt%.
Dokumentation der differentiellen Behandlungsindikation bei depressiven Störungen: Entwicklung und Machbarkeitsprüfung des STEP-D (englisch: Setting Guide for Patients with Depression)
Authors: Depping Miriam , Brünahl ChristianAndreas , Rickert Pascal , Langs Gernot , Löwe Bernd 
Published: April 2018

Zusammenfassung
Ziel der Studie Depressive Störungen erfordern eine differenzierte Behandlung. Zur Dokumentation und Begründung der Indikationsstellung für ein Behandlungssetting wurde ein Algorithmus (STEP-D, englisch: Setting Guide for Patients with Depression) entwickelt und evaluiert.
Methodik Der STEP-D wurde unter Berücksichtigung aktueller Behandlungsleitlinien entwickelt und von 36 Klinikern getestet.
Ergebnisse Besondere Stärken des Instrumentes sind die Nachvollziehbarkeit der Indikationsentscheidung, die Unterstützung der Kommunikation im Behandlungsteam und die verbesserte Dokumentation.
Schlussfolgerung Durch den STEP-D wird der Entscheidungsprozess für ein Behandlungssetting dokumentiert und kann damit die Kommunikation zwischen Patienten, Behandlern und Kostenträgern erleichtern.
Nierentransplantation und Dialyse – Update 2018
Authors: Krüger Bernd , Riegel Werner , Schröppel Bernd 
Published: April 2018

Was ist neu?
Steroidfreie Immunsuppression Der Verzicht auf Steroide nach Nierentransplantation kann einen Diabetes mellitus verhindern, ohne dass ein gehäuftes Auftreten von akuten Abstoßungen bei Patienten mit niedrigem immunologischem Risiko beobachtet werden konnte.
Risiken der Lebendspende Die Lebendnierenspende ist eine wichtige Alternative zur postmortalen Nierenspende, allerdings bestehen perioperative und längerfristige Risiken für den Spender.
Patienteninformation bei Dialyse Seit Jahren ist bekannt, dass die Wahl eines Nierenersatzverfahrens am besten in einem Prozess der partizipativen Entscheidungsfindung (shared decision making) erreicht wird. Nun liegt eine kontrollierte Studie (CORETH-Projekt, Halle) vor. Die Zufriedenheit mit der Verfahrenswahl hängt vom Grad der Information ab; Patientenschulungen wie das Nierenstark-Training der Deutschen Nierenstiftung können eingesetzt werden.
Dialysezugang Der Dialysezugang, Peritonealdialysekatheter und insbesondere der Hämodialyse-Shunt sind mittlerweile als Verfahren standardisiert und weiterentwickelt worden. Das neu-aufgelegte deutschlandweite interdisziplinäre Zertifizierungsverfahren von Shuntzentren bietet weitere qualitative Entwicklungsmöglichkeiten und Expertise.
Dialysattemperatur Die Dialysattemperatur kann zur Kreislaufstabilisierung reduziert werden. In der „slow extended“-Form der Dialysebehandlung ist diese Maßnahme gut etabliert. In der intermittierenden Dialysebehandlung muss auf Nebenwirkungen geachtet werden und zugunsten des gewünschten Effektes abgewogen werden. Der Effekt auf die Dialyseeffizienz ist bislang unklar.
Targeting galectin-1 inhibits pancreatic cancer progression by modulating tumor–stroma crosstalk
Authors: Orozco Carlos A. , Martinez-Bosch Neus , Guerrero Pedro E. , Vinaixa Judith , Dalotto-Moreno Tomás , Iglesias Mar , Moreno Mireia , Djurec Magdolna , Poirier Françoise , Gabius Hans-Joachim , Fernandez-Zapico Martin E. , Hwang Rosa F. , Guerra Carmen , Rabinovich Gabriel A. , Navarro Pilar 
Published: April 2018

Significance
Pancreatic ductal adenocarcinoma (PDA) is the third leading of cause of cancer death in the United States and is predicted to be the second one by 2030. The tumor microenvironment is a major source of soluble mediators that influence tumor progression and hinder the success of therapeutic strategies. Using a genetically engineered mouse model and human cell-based systems, we identify galectin-1 (Gal1) as a critical soluble factor capable of regulating tumor–stroma crosstalk promoting proliferation, angiogenesis, and modulation of inflammatory responses, resulting in enhanced tumor development and metastasis. Our data provide an integrated view of the role of Gal1 in the PDA microenvironment and reinforce the high therapeutic value of Gal1 inhibition in PDA treatment.
3DClusterViSu: 3D clustering analysis of super-resolution microscopy data by 3D Voronoi tessellations
Authors: Andronov Leonid , Michalon Jonathan , Ouararhni Khalid , Orlov Igor , Hamiche Ali , Vonesch Jean-Luc , Klaholz Bruno P 
Published: April 2018

Abstract

Motivation
Single-molecule localization microscopy (SMLM) can play an important role in integrated structural biology approaches to identify, localize and determine the 3D structure of cellular structures. While many tools exist for the 3D analysis and visualization of crystal or cryo-EM structures little exists for 3D SMLM data, which can provide unique insights but are particularly challenging to analyze in three dimensions especially in a dense cellular context.

Results
We developed 3DClusterViSu, a method based on 3D Voronoi tessellations that allows local density estimation, segmentation and quantification of 3D SMLM data and visualization of protein clusters within a 3D tool. We show its robust performance on microtubules and histone proteins H2B and CENP-A with distinct spatial distributions. 3DClusterViSu will favor multi-scale and multi-resolution synergies to allow integrating molecular and cellular levels in the analysis of macromolecular complexes.

Availability and impementation
3DClusterViSu is available under http://cbi-dev.igbmc.fr/cbi/voronoi3D.

Supplementary information
Supplementary figures are available at Bioinformatics online.