bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Proteomic Analysis Reveals Intermediate Stage of Non-Lesional Psoriatic Skin and Points out the Importance of Proteins Outside this Trend
Authors: Szél Edit , Bozó Renáta , Hunyadi-Gulyás Éva , Manczinger Máté , Szabó Kornélia , Kemény Lajos , Bata-Csörgő Zsuzsanna , Groma Gergely 
Published: August 2019

AbstractTo better understand the pathomechanism of psoriasis, a comparative proteomic analysis was performed with non-lesional and lesional skin from psoriasis patients and skin from healthy individuals. Strikingly, 79.9% of the proteins that were differentially expressed in lesional and healthy skin exhibited expression levels in non-lesional skin that were within twofold of the levels observed in healthy and lesional skin, suggesting that non-lesional skin represents an intermediate stage. Proteins outside this trend were categorized into three groups: I. proteins in non-lesional skin exhibiting expression similar to lesional skin, which might be predisposing factors (i.e., CSE1L, GART, MYO18A and UGDH); II. proteins that were differentially expressed in non-lesional and lesional skin but not in healthy and lesional skin, which might be non-lesional characteristic alteration (i.e., CHCHD6, CHMP5, FLOT2, ITGA7, LEMD2, NOP56, PLVAP and RRAS); and III. proteins with contrasting differential expression in non-lesional and lesional skin compared to healthy skin, which might contribute to maintaining the non-lesional state (i.e., ITGA7, ITGA8, PLVAP, PSAPL1, SMARCA5 and XP32). Finally, proteins differentially expressed in lesions may indicate increased sensitivity to stimuli, peripheral nervous system alterations, furthermore MYBBP1A and PRKDC were identified as potential regulators of key pathomechanisms, including stress and immune response, proliferation and differentiation.
Urinary miRNA-27b-3p and miRNA-1228-3p correlate with the progression of Kidney Fibrosis in Diabetic Nephropathy
Authors: Conserva Francesca , Barozzino Mariagrazia , Pesce Francesco , Divella Chiara , Oranger Annarita , Papale Massimo , Sallustio Fabio , Simone Simona , Laviola Luigi , Giorgino Francesco , Gallone Anna , Pontrelli Paola , Gesualdo Loreto 
Published: August 2019

AbstractDiabetic Nephropathy (DN) is a chronic complication of diabetes and the primary cause of end stage renal disease. Differential diagnosis for DN requires invasive histological investigation, thus there is need for non-invasive biomarkers to discriminate among different histological lesions in diabetic patients. With the aim to identify a pattern of differentially expressed miRNAs in kidney biopsies of DN patients, we assayed miRNA expression in kidney biopsies from DN patients, diabetic patients with membranous nephropathy and patients with normal histology. Nine miRNAs were differentially expressed among the three groups, and 2 miRNAs (miR-27b-3p and miR-1228-3p) showed interaction with an ubiquitin-conjugating E2 enzyme variant (UBE2v1). UBE2v1 mediates the formation of lysine 63-linked ubiquitin chains, a mechanism we previously showed as involved in DN kidney fibrosis. Both miRNAs were validated as down-regulated in biopsies and urines of DN patients, possibly affected by DNA methylation. Interestingly, the urinary levels of both miRNAs could also discriminate among different degrees of renal fibrosis. Finally, we showed that the combined urinary expression of both miRNAs was also able to discriminate DN patients from other glomerulonephritides in diabetic patients. In conclusion we identified two miRNAs potentially useful as candidate biomarkers of tubular-interstitial fibrosis in diabetic patients with DN.
Thermostability as a highly dependent prion strain feature
Authors: Marín-Moreno Alba , Aguilar-Calvo Patricia , Moudjou Mohammed , Espinosa Juan Carlos , Béringue Vincent , Torres Juan María 
Published: August 2019

AbstractPrion diseases are caused by the conversion of physiological PrPC into the pathogenic misfolded protein PrPSc, conferring new properties to PrPSc that vary upon prion strains. In this work, we analyze the thermostability of three prion strains (BSE, RML and 22L) that were heated at 98 °C for 2 hours. PrPSc resistance to proteinase K (PrPres), residual infectivity by mouse bioassay and in vitro templating activity by protein misfolding cyclic amplification (PMCA) were studied. Heated strains showed a huge loss of PrPres and a radically different infectivity loss: RML was the most thermolabile strain (6 to 7 log10 infectivity loss), followed by 22L (5 log10) while BSE was the most thermostable strain with low or null infectivity reduction showing a clear dissociation between PrPres and infectivity. These results indicate that thermostability is a strain-specific feature, measurable by PMCA and mouse bioassay, and a great tool to distinguish prion strains.
Population genetics of ectoparasitic mites suggest arms race with honeybee hosts
Authors: Beaurepaire Alexis L. , Moro Arrigo , Mondet Fanny , Le Conte Yves , Neumann Peter , Locke Barbara 
Published: August 2019

AbstractThe ectoparasitic mite, Varroa destructor, is the most severe biotic threat to honeybees (Apis mellifera) globally, usually causing colony death within a few years without treatments. While it is known that a few A. mellifera populations survive mite infestations by means of natural selection, the possible role of mite adaptations remains unclear. To investigate potential changes in mite populations in response to host adaptations, the genetic structure of V. destructor in the mite-resistant A. mellifera population on Gotland, Sweden, was studied. Spatio-temporal genetic changes were assessed by comparing mites collected in these colonies, as well as from neighboring mite-susceptible colonies, in historic (2009) and current (2017/2018) samples. The results show significant changes in the genetic structure of the mite populations during the time frame of this study. These changes were more pronounced in the V. destructor population infesting the mite-resistant honeybee colonies than in the mite-susceptible colonies. These results suggest that V. destructor populations are reciprocating, in a coevolutionary arms race, to the selection pressure induced by their honeybee host. Our data reveal exciting new insights into host-parasite interactions between A. mellifera and its major parasite.
A Design of Experiments (DoE) Approach Accelerates the Optimization of Copper-Mediated 18F-Fluorination Reactions of Arylstannanes
Authors: Bowden Gregory D. , Pichler Bernd J. , Maurer Andreas 
Published: August 2019

AbstractRecent advancements in 18F radiochemistry, such as the advent of copper-mediated radiofluorination (CMRF) chemistry, have provided unprecedented access to novel chemically diverse PET probes; however, these multicomponent reactions have come with a new set of complex optimization problems. Design of experiments (DoE) is a statistical approach to process optimization that is used across a variety of industries. It possesses a number of advantages over the traditionally employed “one variable at a time” (OVAT) approach, such as increased experimental efficiency as well as an ability to resolve factor interactions and provide detailed maps of a process’s behavior. Here we demonstrate the utility of DoE to the development and optimization of new radiochemical methodologies and novel PET tracer synthesis. Using DoE to construct experimentally efficient factor screening and optimization studies, we were able to identify critical factors and model their behavior with more than two-fold greater experimental efficiency than the traditional OVAT approach. Additionally, the use of DoE allowed us to glean new insights into the behavior of the CMRF of a number of arylstannane precursors. This information has guided our decision-making efforts while developing efficient reaction conditions that suit the unique process requirements of 18F PET tracer synthesis.
Hyperosmolar environment and intestinal epithelial cells: impact on mitochondrial oxygen consumption, proliferation, and barrier function in vitro
Authors: Grauso Marta , Lan Annaïg , Andriamihaja Mireille , Bouillaud Frédéric , Blachier François 
Published: August 2019

AbstractThe aim of the present study was to elucidate the in vitro short-term (2-h) and longer-term (24-h) effects of hyperosmolar media (500 and 680 mOsm/L) on intestinal epithelial cells using the human colonocyte Caco-2 cell line model. We found that a hyperosmolar environment slowed down cell proliferation compared to normal osmolarity (336 mOsm/L) without inducing cell detachment or necrosis. This was associated with a transient reduction of cell mitochondrial oxygen consumption, increase in proton leak, and decrease in intracellular ATP content. The barrier function of Caco-2 monolayers was also transiently affected since increased paracellular apical-to-basal permeability and modified electrolyte permeability were measured, allowing partial equilibration of the trans-epithelial osmotic difference. In addition, hyperosmotic stress induced secretion of the pro-inflammatory cytokine IL-8. By measuring expression of genes involved in energy metabolism, tight junction forming, electrolyte permeability and intracellular signaling, different response patterns to hyperosmotic stress occurred depending on its intensity and duration. These data highlight the potential impact of increased luminal osmolarity on the intestinal epithelium renewal and barrier function and point out some cellular adaptive capacities towards luminal hyperosmolar environment.
Variants Associated with the Ankle Brachial Index Differ by Hispanic/Latino Ethnic Group: a genome-wide association study in the Hispanic Community Health Study/Study of Latinos
Authors: Sofer Tamar , Emery Leslie , Jain Deepti , Ellis Alicia M. , Laurie Cathy C. , Allison Matthew A. , Lee Jiwon , Kurniansyah Nuzulul , Kerr Kathleen F. , González Hector M. , Tarraf Wassim , Criqui Michael H. , Lange Leslie A. , Palmas Walter R. , Franceschini Nora , Wassel Christina L. 
Published: August 2019

AbstractLower extremity peripheral artery disease (PAD) burden differs by race/ethnicity. Although familial aggregation and heritability studies suggest a genetic basis, little is known about the genetic susceptibility to PAD, especially in non-European descent populations. Genome-wide association studies (GWAS) of the ankle brachial index (ABI) and PAD (defined as an ABI < 0.90) have not been conducted in Hispanics/Latinos. We performed a GWAS of PAD and the ABI in 7,589 participants aged >45 years from the Hispanic Community Health Study/Study of Latinos (HCHS/SOL). We also performed GWAS for ABI stratified by Hispanic/Latino ethnic subgroups: Central American, Mexican, and South American (Mainland group), and Cuban, Dominican, and Puerto Rican (Caribbean group). We detected two genome-wide significant associations for the ABI in COMMD10 in Puerto Ricans, and at SYBU in the Caribbean group. The lead SNP rs4466200 in the COMMD10 gene had a replication p = 0.02 for the ABI in Multi-Ethnic Study of Atherosclerosis (MESA) African Americans, but it did not replicate in African Americans from the Cardiovascular Health Study (CHS). In a regional look-up, a nearby SNP rs12520838 had Bonferroni adjusted p = 0.05 (unadjusted p = 7.5 × 10−5) for PAD in MESA Hispanics. Among three suggestive associations (p < 10−7) in subgroup-specific analyses, DMD on chromosome X, identified in Central Americans, replicated in MESA Hispanics (p = 2.2 × 10−4). None of the previously reported ABI and PAD associations in whites generalized to Hispanics/Latinos.
Transparency induced in opals via nanometer thick conformal coating
Authors: Shang Guoliang , Furlan Kaline Pagnan , Zierold Robert , Blick Robert H. , Janßen Rolf , Petrov Alexander , Eich Manfred 
Published: August 2019

AbstractSelf-assembled periodic structures out of monodisperse spherical particles, so-called opals, are a versatile approach to obtain 3D photonic crystals. We show that a thin conformal coating of only several nanometers can completely alter the reflection properties of such an opal. Specifically, a coating with a refractive index larger than that of the spherical particles can eliminate the first photonic band gap of opals. To explain this non-intuitive effect, where a nm-scaled coating results in a drastic change of optical properties at wavelengths a hundred times bigger, we split the permittivity distribution of the opal into a lattice function convoluted with that of core-shell particles as a motif. In reciprocal space, the Bragg peaks that define the first Brillouin zone can be eliminated if the motif function, which is multiplied, assumes zero at the Bragg peak positions. Therefore, we designed a non-monotonic refractive index distribution from the center of the particle through the shell into the background and adjusted the coating thickness. The theory is supported by simulations and experiments that a nanometer thin TiO2 coating via atomic layer deposition (ALD) on synthetic opals made from polystyrene particles induces nearly full transparency at a wavelength range where the uncoated opal strongly reflects. This effect paves the way for sensing applications such as monitoring the thicknesses growth in ALD in-situ and in real time as well as measuring a refractive index change without spectral interrogation.
Morphological Awareness Strategies to Promote Academic Success at Tier 1 Through Interprofessional Collaboration
Authors: Collins Ginger , Wolter Julie A. 
Published: August 2019

Purpose
Many school-based speech-language pathologists (SLPs) are interested in interprofessional collaborations with teachers to provide classroom-based services but are often unsure how to design and implement these services in a way that will meet teachers' curricular standards and serve the specific language needs of students. The purpose of this article is to provide SLPs an interprofessional model for providing quality classroom-based language–literacy instruction that facilitates language success for children with and without language–literacy deficits using a morphological awareness strategy approach.

Conclusion
The 8-week teacher–SLP collaborative classroom model focused on morphological awareness to improve 3rd-grade vocabulary, reading, and writing presented in this article provides a framework for implementing classroom-based services relevant to the curriculum.
ZnO nanorod‐based Love wave delay line for high mass sensitivity: a finite element analysis
Authors: Trivedi Shyam , Nemade Harshal B. 
Published: August 2019

This study presents finite element time‐domain simulation of a ‐YX LiTaO3 Love wave (LW) delay line to explore coupled resonance phenomenon with ZnO nanorods designed on the top surface of the device. The effect of variation in ZnO nanorods height on the propagation of LW, mode‐transitions, area‐averaged stress, insertion loss, and mass sensitivity of the device is studied. Simulation results show that at the critical height of ZnO nanorods, coupled resonance occurs that causes a sharp swing in the phase shift indicating a transition from inertial to elastic loading. Coupled resonance increases the acoustic impedance of the device and also the average stress between nanorod and substrate interface. Simulation of mass loading is performed by applying an incremental surface mass density on the active area of the device. A nanorod packing density of gives a high mass sensitivity of which is more than ten times the sensitivity of a plain LW device. At the coupled resonant height, the insertion loss of the sensor increases, and a swing of about 1 dB is observed with variation in the nanorod height.
Au nanoparticles by instant green chemicals and optimising key synthesis parameters using Taguchi method
Authors: Korukonda Jagajjanani Rao , Korumilli Tarangini 
Published: August 2019

In the recent years, functionalised Au nanoparticles using eco‐friendly chemical routes are under the focus for developing several technological applications. However, a simple, reproducible, and controlled synthesis of high‐quality nanoparticles is challenging in these approaches. In this work, readily available green chemicals were utilised and the usefulness of and Taguchi approach for optimisation of synthesis procedure was designed for the synthesis of possible lower size Au nanoparticles of size 93 ± 7 nm. L16 orthogonal array, by which the influence of four factors in four levels may well be tested, was chosen. Analysis of variance was done on the attained results and optimum condition suggested by statistical estimations was tested through a verification test. The identified key parameters for synthesising lower size Au nanoparticles are reducing agent (curcumin + cinnamic acid + citric acid), additive (zero), sonication time (30 min), and temperature (50°C) after performing the optimisation technique. Furthermore, this study also provides a selective approach to obtain different shape/shape Au nano to colloidal structures through different trail selection within the Taguchi experimental array.
Final spin states of eccentric ocean planets
Authors: Auclair-Desrotour P. , Leconte J. , Bolmont E. , Mathis S. 
Published: August 2019

Context. Eccentricity tides generate a torque that can drive an ocean planet towards asynchronous rotation states of equilibrium when enhanced by resonances associated with the oceanic tidal modes.
Aims. We investigate the impact of eccentricity tides on the rotation of rocky planets hosting a thin uniform ocean and orbiting cool dwarf stars such as TRAPPIST-1, with orbital periods ~1−10 days.
Methods. Combining the linear theory of oceanic tides in the shallow water approximation with the Andrade model for the solid part of the planet, we developed a global model including the coupling effects of ocean loading, self-attraction, and deformation of the solid regions. From this model we derive analytic solutions for the tidal Love numbers and torque exerted on the planet. These solutions are used with realistic values of parameters provided by advanced models of the internal structure and tidal oscillations of solid bodies to explore the parameter space both analytically and numerically.
Results. Our model allows us to fully characterise the frequency-resonant tidal response of the planet, and particularly the features of resonances associated with the oceanic tidal modes (eigenfrequencies, resulting maxima of the tidal torque, and Love numbers) as functions of the planet parameters (mass, radius, Andrade parameters, ocean depth, and Rayleigh drag frequency). Resonances associated with the oceanic tide decrease the critical eccentricity beyond which asynchronous rotation states distinct from the usual spin-orbit resonances can exist. We provide an estimation and scaling laws for this critical eccentricity, which is found to be lowered by roughly one order of magnitude, switching from ~0.3 to ~0.06 in typical cases and to ~0.01 in extremal ones.
Prävalenz und Schwere eines Tinnitus bei
Otosklerose
Authors: Hesse Gerhard 
Published: August 2019

Dziendziel B et al. Prevalence and severity of tinnitus in Polish otosclerosis
patients qualified for stapes surgery. Eur Arch Otorhinolaryngol 2019; doi:
10.1007/s00405–019–05317–8
Polnische Wissenschaftler beurteilten Prävalenz und Schwere eines Tinnitus bei
einer Gruppe von für eine Stapesoperation geeigneten Patienten mit
Otosklerose. Ein sekundäres Ziel war es, den Zusammenhang zwischen der
Schwere des Tinnitus und der Hörschwelle zu bestimmen.
Hintergründe der neuen
Qualitätsprüfungs-Richtlinien
Authors: Fajardo Annemarie 
Published: August 2019

ZusammenfassungDer Einstieg in das neue System der Qualitätsprüfungs-Richtlinien (QPR) beginnt
zum Sommer 2019. Seit Ende 2018 gibt es gesetzliche Vorgaben, die den
Übergang in das neue Indikatorensystem regeln. Hier einige Tipps und
Hintergründe.
Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.) seeds
Authors: Fan Sanhong , Li Jiani , Bai Baoqing 
Published: August 2019

ABSTRACT
Quinoa crude polysaccharides (QPS) were extracted from Chenopodium quinoa Willd. The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials. Its chemical structure was identified using FT-IR, NMR, AFM, SEM and Congo red staining. High performance gel permeation chromatography (HPGPC) was used to determine molecular weight, and composition by HPLC. QPS1, with a molecular weight of 34.0 kDa, was mainly composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose at a molar ratio of 2.63:2.40:1.64:6.28:1.95:2.48:5.01. In addition, we evaluated the ameliorative effects of QPS1 on the improvement of anti-cyclophosphamide (CTX)-induced immunosuppression in ICR mice. The result exhibited significantly immune-enhancing activity: QPS1 successfully improved the content of IFN-γ, IL-6, IFN-ɑ, IgM and lysozyme (LYSO) in serum for three weeks, enhanced the phagocytic function of mononuclear macrophages and ameliorated delayed allergy in mice.
Short‐term high‐Intensity interval training increases systemic brain‐derived neurotrophic factor (BDNF) in healthy women
Authors: Rentería Iván , García‐Suárez Patricia C. , Martínez‐Corona David O. , Moncada‐Jiménez José , Plaisance Eric P. , JiméNez‐Maldonado Alberto 
Published: August 2019

AbstractBackground: Brain‐derived neurotrophic factor (BDNF) increases neuronal viability and cognitive function, peripheral lipid metabolism and skeletal muscle repair. The primary purpose of this study was to determine the effect of short‐term high‐intensity interval training (HIIT) on serum BDNF concentrations in healthy young women. Methods: Seventeen women (age:22 ± 1 years); body mass index (BMI:24.2 ± 2.2 kg/m²), body fat percentage (% fat:25.8 ± 4.7) participated in the study. Participants were randomly assigned to a control (n = 8) or HIIT group (n = 9). All participants performed a graded exercise test (GXT) on an electronically‐braked cycle ergometer to determine maximal aerobic power (MAP, Watts). HIIT was performed three days per week for four weeks. Each HIIT session consisted of three to five cycling bouts of 30 s each at 80% MAP, followed by four‐minutes of recovery at 40% MAP. Forty‐eight hours after the last bout of exercise, both groups performed a follow‐up GXT. Non‐fasting blood samples were collected before and immediately after each GXT. Mixed factorial (2 groups x 4 measures, and 2 groups x 2 measures) ANOVA was used to assess BDNF concentrations, performance and anthropometric variables. Results: Serum BDNF concentrations in the HIIT group (21.9 ± 1.3 ng/mL) increased compared to control (19.2 ± 2.8 ng/mL) (∼12%, P < 0.05) following HIIT. In contrast, circulating BDNF concentrations were reduced following the GXT (P < 0.05). The MAP and % Fat did not change with HIIT. Conclusions: Twelve sessions of HIIT increases circulating BDNF concentrations in healthy young women despite no change in physical performance or % fat.
Qualitative tool for designing natural protected areas and ecological restoration projects
Authors: Pan Xubin 
Published: August 2019

ABSTRACT
The rotational symmetry connection between the Species-Area Relationship (SAR) and Endemics-Area Relationship (EAR) was discovered in the frame of Species-Area Theory. In this paper, this paired connection was applied into the impact of variation of number of species and area and dynamic change of species-abundance distribution on the curve shape of SAR and EAR, which can be integrated into the assessment of Natural Protected Areas and Ecological Restoration Projects. The results indicate that the underestimate or overestimate of total number of species can lead to the underestimate or overestimate of extinction rate, while the reduction or expansion of area can lead to the increase or decrease in extinction rate. In addition, the species-abundance distribution change of community causes the shape tuning of SAR and EAR, which leads to inconsistent change of extinction rate. Thus, this SAT frame can be used as a qualitative tool in conservation and restoration management.