bluuebunny/crossref_metadata_2025_split huggingface dataset:
An E460D substitution in the NS5 protein of tick-borne encephalitis virus confers resistance to the inhibitor Galidesivir (BCX4430) and also attenuates the virus for mice
Authors: Eyer Ludek , Nougairède Antoine , Uhlířová Marie , Driouich Jean-Sélim , Zouharová Darina , Valdés James J. , Haviernik Jan , Gould Ernest A. , De Clercq Erik , de Lamballerie Xavier , Ruzek Daniel 
Published: February 2019

AbstractThe adenosine analogue Galidesivir (BCX4430), a broad-spectrum RNA virus inhibitor, has entered a Phase 1 clinical safety and pharmacokinetics study in healthy subjects and is under clinical development for treatment of Ebola virus infection. Moreover, Galidesivir also inhibits the reproduction of tick-borne encephalitis virus (TBEV) and numerous other medically important flaviviruses. Until now, studies of this antiviral agent have not yielded resistant viruses. Here, we demonstrate that an E460D substitution, in the active site of TBEV RNA-dependent-RNA-polymerase (RdRp), confers resistance to Galidesivir in cell culture. Stochastic molecular simulations indicate that the steric freedom caused by the E460D substitution increases close electrostatic interactions between the inhibitor and the interrogation residue of the TBEV RdRp motif F, resulting in rejection of the analogue as an incorrect/modified nucleotide. Galidesivir-resistant TBEV exhibited no cross-resistance to structurally different antiviral nucleoside analogues, such as 7-deaza-2’-C-methyladenosine, 2’-C-methyladenosine and 4’-azido-aracytidine. Although, the E460D substitution led only to a subtle decrease in viral fitness in cell culture, Galidesivir-resistant TBEV was highly attenuated in vivo, with 100% survival rate and no clinical signs observed in infected mice. Our results contribute to understanding the molecular basis of Galidesivir antiviral activity, flavivirus resistance to nucleoside inhibitors and the potential contribution of viral RdRp to flavivirus neurovirulence.ImportanceTick-borne encephalitis virus (TBEV) is a pathogen that causes severe human neuroinfections in large areas of Europe and Asia and for which there is currently no specific therapy. We have previously found that Galidesivir (BCX4430), a broad-spectrum RNA virus inhibitor, which is under clinical development for treatment of Ebola virus infection, has a strong antiviral effect against TBEV. For any antiviral drug, it is important to generate drug-resistant mutants to understand how the drug works. Here, we produced TBEV mutants resistant to Galidesivir and found that the resistance is caused by a single amino acid substitution in an active site of the viral RNA-dependent RNA polymerase, an enzyme which is crucial for replication of viral RNA genome. Although, this substitution led only to a subtle decrease in viral fitness in cell culture, Galidesivir-resistant TBEV was highly attenuated in a mouse model. Our results contribute to understanding the molecular basis of Galidesivir antiviral activity.
UDCT: Unsupervised data to content transformation with histogram-matching cycle-consistent generative adversarial networks
Authors: Ihle Stephan , Reichmuth Andreas M. , Girardin Sophie , Han Hana , Stauffer Flurin , Bonnin Anne , Stampanoni Marco , Vörös János , Forró Csaba 
Published: February 2019

AbstractThe segmentation of images is a common task in a broad range of research fields. To tackle increasingly complex images, artificial intelligence (AI) based approaches have emerged to overcome the shortcomings of traditional feature detection methods. Owing to the fact that most AI research is made publicly accessible and programming the required algorithms is now possible in many popular languages, the use of such approaches is becoming widespread. However, these methods often require data labeled by the researcher to provide a training target for the algorithms to converge to the desired result. This labeling is a limiting factor in many cases and can become prohibitively time consuming. Inspired by Cycle-consistent Generative Adversarial Networks’ (cycleGAN) ability to perform style transfer, we outline a method whereby a computer generated set of images is used to segment the true images. We benchmark our unsupervised approach against a state-of-the-art supervised cell-counting network on the VGG Cells dataset and show that it is not only competitive but can also precisely locate individual cells. We demonstrate the power of this method by segmenting bright-field images of cell cultures, a live-dead assay of C.Elegans and X-ray-computed tomography of metallic nanowire meshes.
Erythropoiesis differences in various clinical phases of Dengue fever using Immature Reticulocyte Fraction parameter
Authors: Oehadian Amaylia , Vidyaniati Putri , Candra Jeffery Malachi , Sumardi Uun , Susandi Evan , Alisjahbana Bachti 
Published: February 2019

AbstractDengue fever is the most common cause of hospitalization in otherwise healthy person. The incidence rate increases significantly every year, from 0.05 per 100.000 inhabitants in 1968 to 35-40 per 100.000 inhabitants in 2013. Thrombocytopenia and leucopenia are the most commonly reported changes, whether they are caused by bone marrow suppression or peripheral destruction. Anemia has also been observed to occur in dengue infection with unknown mechanism. Immature Reticulocyte Fraction (IRF) is a parameter reflecting the most immature reticulocyte fraction and it can identify the earliest stage of erythropoiesis disorder. This research aimed to determine the mechanism of erythropoiesis disorders that led to anemia using IRF parameter in the various clinical phases of dengue fever. This study was a comparative analytical research using secondary data derived from theDengue-associated Endothelial Cell Dysfunction and Thrombocyte Activation(DECENT) research. The baseline characteristic data consists of sex, age, level of hemoglobin, hematocrit, leucocyte, and thrombocyte, also the IRF. The patients were grouped into the fever, critical, recovery, and convalescent phases, plus healthy control group. The data was analyzed using the Kolmogorov-Smirnov normality test, followed by Friedman test and Mann-Whitney post hoc test. There were 244 research subjects and the median age was 24 (14-67), with similar ratio of male and female. The median IRF for all the research subjects was 4.8% with an IQR of 2.4-8.1%. The fever-phase group showed a median of 1.8% with an IQR of 0.5-2.85%. The critical-phase group showed a median of 3.6% with an IQR of 1.8- 5.0%, while the median for the recovery-phase group was 7.05% with an IQR of 4.08-11.85%. The convalescent-phase group showed a median of 7.3 % with an IQR of 3.95-9.3%, and the healthy-control group showed a median of 4.1% with an IQR of 2.2-6.6%. There was a significant difference in IRF between the groups (p<0.05). Immature Reticulocyte Fraction in fever phase was significantly different with IRF in other phases and healthy controls (p<0.05). In the critical phase, IRF was not significantly different from the healthy controls (p=0.218). Summarizing, there are changes in erythropoiesis activity in various clinical phases of dengue infection. Erythropoiesis suppression occurred mainly during the fever phase and it started to restore in the critical phase. In the recovery and convalescent phases, the erythropoiesis activity was increased.
Teaching innovations in Asian higher education: perspectives of educators
Authors: Wong T.M. 
Published: February 2019

Purpose
The purpose of this paper is to identify the teaching innovations that have been implemented in higher education institutions in Asia and the perspectives of educators on them.

Design/methodology/approach
Semi-structured interviews were conducted with 28 educators who were affiliated with 23 higher education institutions in ten Asian countries/regions. The interviews covered information about the teaching innovations of the participants’ institutions, the characteristics of the innovative practices and the participants’ views on them. The relationships between the characteristics of institutions and their teaching innovations were also examined.

Findings
The results showed that the teaching innovations included two main categories, namely, those which involved the use of advanced technologies and those which did not. The innovations that involved the use of advanced technologies were mainly from larger institutions, while the other category was mainly from smaller ones and had been practised for less than 1.5 years. Differences were also identified between the two categories in terms of the aims and importance of innovations, innovative features, the evaluation of innovations and improvements needed for them.

Originality/value
The results highlighted that technology is only one of the many aspects of teaching innovations, which is different from the view prevailing in the literature. They also suggested that differences in the scale of institutions (in terms of number of students) possibly influences the kind of teaching innovations adopted.
Drivers for construction productivity
Authors: Seadon Jeff , Tookey John E. 
Published: February 2019

Purpose
The New Zealand construction sector is similar to many other countries with a few large companies and many small and micro enterprises. It seeks to achieve a 20 per cent increase in productivity by 2020 which requires a step change in how the sector operates and buy-in from key stakeholders. The purpose of this paper is to provide a set of levers to improve productivity in the construction sector and develop an implementation schedule.

Design/methodology/approach
This paper adopts a systems approach taking account of the nature of the building sector and the whole life cycle of a building from design to end-of-life. Information gained from the post-construction phases informs the pre-construction and construction phases.

Findings
Productivity is an integrated model whereby increases in process efficiency are executed with quality materials and workmanship, in a manner that is affordable for both the client and contractor and sustainable over time. A series of interviews and workshops produced 10 nodal points and 19 crucial levers which were prioritised for implementation. Additionally, indicators were developed to monitor progress over time and provide information for further corrective action to the system.

Practical implications
The effect of using a few targeted levers in unison provided significantly more gains than individual applications. Modelling real world responses to process stimuli outlined in this paper is extremely valuable. This provided the opportunity for key construction stakeholders to estimate the effects of decision making during a project.

Originality/value
Previous studies identified factors affecting productivity. Piecemeal approaches to improve productivity have resulted in systemic failure. A whole of life approach provides valuable insights to improve productivity in the construction and pre-construction phases which have a flow-on effect through the life cycle. Importantly, this research proposes drivers, an implementation scheme and indicators that provide leverage on nodal points to improve productivity.
Paternalistic leadership and innovation: the moderating effect of environmental dynamism
Authors: Hou Bojun , Hong Jin , Zhu Kejia , Zhou Yu 
Published: February 2019

PurposeThe purpose of this paper is to focus on how the three elements of paternalistic leadership – authoritarianism, benevolence and moral leadership – affect organizational innovation – both explorative and exploitative innovation – in Chinese enterprises. It also examines the moderating effect of environmental dynamism on the relationship between paternalistic leadership and organizational innovation.Design/methodology/approachData on 190 superior–subordinate dyads are collected using questionnaire surveys. The supervisors are recruited from the MBA program in a famous university in the city of Hefei, China, who are also asked to distribute subordinate questionnaires to their subordinates. The hierarchical regression analysis is conducted to test the hypotheses by using SPSS 22.0.FindingsThe analysis of 190 superior–subordinate dyads shows that benevolent and authoritarian leadership is positively related to exploratory innovation, while moral leadership has no significant impact on exploratory innovation. The results also reveal that all three elements of paternalistic leadership is, in general, positively correlated with exploitative innovation. Furthermore, environmental dynamism moderates the relationship between paternalistic leadership and innovation. In a dynamic environment, moral leadership has a stronger positive effect on innovation, but only on exploratory innovation; whereas authoritarian leadership exerts more detrimental effects on both exploratory and exploitative innovation.Originality/valueThe current work contributes to understanding the relationship between paternalistic leadership and innovation in the Chinese cultural context by examining the effects of the three elements of paternalistic leadership separately and by showing how these effects can be moderated by environmental dynamism.
Assessing the relevance of digital competences on business graduates’ suitability for a job
Authors: Periáñez-Cañadillas Iñaki , Charterina Jon , Pando-García Julián 
Published: February 2019

PurposeThe purpose of this paper is to examine the role of digital competences in relation to the general competences demanded by the job market in the case of Business graduates’ future.Design/methodology/approachA telephone survey was conducted with 992 team and HR managers from November 15 to December 15, 2016. The selected respondents were chosen from a sampling frame of 4,880 registered members of a professional association of Business and Economics graduates. Only those admitting to have or having had employees under their command were accountable. Thus, the final sample of the analysis comprised a total of 231 respondents. We used partial least square modeling in order to test the hypotheses.FindingsThe study carried out in the professional field of graduates in Business studies shows us that of the five dimensions analyzed as digital competences, four of them show an influence on the candidate’s suitability for the post. These are communication, content creation, safety and problem solving. In the variables related to information, this relationship is not observed.Originality/valueIn this research, the authors analyze the digital competences from the perspective of the possible employer. The concept of digital competence is of increasing importance in recent research. There is a need to relate the competences demanded by the companies and those developed by the training centers. Also, prior literature is limited in the number of works analyzing digital competences specifically in the professional activities concerning graduates in Economics of Business studies.
Networked library access and services delivery for the blind and visually impaired university students: evidence from the UAE
Authors: Al-Harrasi Alia , Taha Ahmed 
Published: February 2019

Purpose
The study aims to discuss the special needs-centric information delivery as a new genre of the academic library services. It also sheds light on the assistive technology used to help blind or visually impaired (BVI) students to access the available digital contents and use online services conveniently. A particular emphasis placed on gaining some insights into BVIs’ perceptions towards the orientation and assistance offered by the access service librarians, as well as any experience in using assistive educational technology for reading and information access.

Design/methodology/approach
The authors used semi-structured interview survey to gather the necessary data from 35 BVI university students drawn from the Special Needs Services (SNS) Unit at the UAE University. The interview was concerned with use of library e-resources and online services for doing their assignments, easy-to-use facilities to access the e-resources and main challenges facing them in communicating with the librarians.

Findings
The findings revealed that the BVI students considered that the university library does not answer their needs for getting access to digital content and using online services adequately.

Practical implications
The Main Library should cooperate closely with the SNS Unit to get the most benefits from their experience in the BVI-devoted services to develop an efficient library information discovery and delivery to this student group.

Originality/value
There is scarcity of scholarly studies on the pedagogical and information acquisition challenges facing the BVI university students in the UAE higher education. This is the first research study that tackles the experience of the BVI students in using the academic library.
Factors influencing consumer loyalty: evidence from the Ghanaian retail banking industry
Authors: Omoregie Osaretin Kayode , Addae John Agyekum , Coffie Stanley , Ampong George Oppong Appiagyei , Ofori Kwame Simpe 
Published: February 2019

PurposeThe increasing number of banks in the Ghanaian banking industry has brought about intense competition in the industry. The purpose of this paper is, therefore, to examine the factors that influence retail banking customers’ loyalty intentions.Design/methodology/approachIn order to validate the proposed research model, the study adopts a survey design. Data were collected from 565 customers of the top performing banks in terms of customer deposits. Data analysis employed the partial least squares structural equation modeling (PLS–SEM) using SmartPLS version 3.FindingsResults from the PLS–SEM analysis indicated that satisfaction, service quality and trust had significant effect on loyalty, with satisfaction having the most significant effect. Interestingly corporate image was found to have a significant effect on both satisfaction and trust but not on loyalty. In all, the proposed model accounted for 63.3 percent of the variation in loyalty.Research limitations/implicationsThe current study samples customers from only the top performing banks in Ghana. The use of cross-sectional data makes it impossible to study how customers’ perceptions change over time. Results from this study could, however, help managers of banks in designing strategies aimed at improving customer loyalty in order to consolidate their market share.Originality/valueThis paper adds to existing works that focus on loyalty in the retail banking sector, especially from the context of a developing economy. The study draws attention to the interrelationship among service quality, perceived value, satisfaction, image, trust and loyalty.
Denying homeless persons access to municipal support
Authors: Boesveldt Nienke Fredrika 
Published: February 2019

Purpose
The purpose of this paper is to focus on the denial of social support to homeless persons and related societal effects of new local governance arrangements.

Design/methodology/approach
Analysis of new data and secondary evaluative and comparative data on the policies, administrative structures and management styles of Copenhagen, Glasgow and Amsterdam have brought better understanding of the elements of local governance arrangements that influence the number of homeless persons who are denied access to services and the number of persons sleeping rough who are not eligible for social support. Theoretical explanations for the impact of governance arrangements on these processes and societal effects are considered.

Findings
It appears that while the body of research, reports and policy documents on non-eligibility for homelessness services is growing, legal responses at best remain vague, and policies are still in the process of being developed. Modest progress on policy goals, and even more so on policy instruments, leading to less detrimental outcomes, can be explained by centralising and decentralising trends and the relationships between state and society. The latter may also be indicative of how the increased focus on the legal problems of some EU migrants can be explained.

Research limitations/implications
The two points in time documented for the case studies are relevant in understanding processes underlying the current circumstances of homeless persons and homeless migrants and offer an interdisciplinary insight into governance and politics, law, and public and health service perspectives.

Social implications
Good policy practice, as this paper shows, can lead to a difference in individual lives.

Originality/value
Much is unknown about considerations inside government. This paper contributes by combining theoretical and insider perspectives.
The influence of a board’s ethical commitment on corporate governance in enhancing a company’s corporate performance
Authors: Salin Ahmad Saiful Azlin Puteh , Ismail Zubaidah , Smith Malcolm , Nawawi Anuar 
Published: February 2019

PurposeThe purpose of this paper is to examine the relationship between corporate governance and company performance and how a board’s ethical commitment can influence this relationship. Prior studies documented mixed evidence on the corporate governance and corporate performance relationship, which can be due to the influence of a board’s ethical commitment and will shape the corporate governance mechanism in the company and, in turn, influence performance.Design/methodology/approachThis study collected data for two years, i.e. 2013 and 2014, from the biggest 500 Malaysian companies listed in the stock exchange. Corporate governance is measured based on the requirements of the Malaysian Code of Corporate Governance (MCCG), while a board’s ethical commitment is measured based on the MCCG and various international best practices. Corporate performance is measured based on return on equity, return on assets, net profit margin, market-to-book value and TobinQ.FindingsA board’s ethical commitment was found to be significant in increasing the strength of the relationship between corporate governance and corporate performance. The findings are robust to the alternative performance measurements and lagged one-year corporate performance.Research limitations/implicationsThis paper provides further evidence on the importance of ethical practices to improve corporate environment and, hence, sustain a company’s performance. This study, however, was conducted on only large companies with a limited data collection period.Practical implicationsThis study provides an indicator that the policymaker and regulatory authorities need to double their efforts in promoting and encouraging a board of directors to take a bold step in improving its ethical culture. Shareholders and investors need to use their power and rights to demand the company to improve their governance and ethical practices.Originality/valueThis study is original, as it measures a board’s ethical commitment from various sources of local and international best practices such as Malaysia, Australia, Canada, Norway, South Korea, Singapore, Sweden, Turkey, the UK and the USA. It also contributes to the literature and theoretical understanding of the interaction between a board’s ethical commitment and corporate governance on corporate performance, particularly in developing countries like Malaysia, which is scarce in the literature.