bluuebunny/crossref_metadata_2025_split huggingface dataset:
New type of interaction between the SARAH domain of the tumour suppressor RASSF1A and its mitotic kinase Aurora A
Authors: Szimler T. , Gráczer É. , Györffy D. , Végh B. , Szilágyi A. , Hajdú I. , Závodszky P. , Vas M. 
Published: April 2019

AbstractThe tumour suppressor protein RASSF1A is phosphorylated by Aurora A kinase, thereby impairing its tumour suppressor function. Consequently, inhibiting the interaction between Aurora A and RASSF1A may be used for anti-tumour therapy. We used recombinant variants of RASSF1A to map the sites of interaction with Aurora A. The phosphorylation kinetics of three truncated RASSF1A variants has been analysed. Compared to the RASSF1A form lacking the 120 residue long N-terminal part, the Km value of the phosphorylation is increased from 10 to 45 μM upon additional deletion of the C-terminal SARAH domain. On the other hand, deletion of the flexible loop (Δ177–197) that precedes the phosphorylation site/s (T202/S203) results in a reduction of the kcat value from about 40 to 7 min−1. Direct physical interaction between the isolated SARAH domain and Aurora A was revealed by SPR. These data demonstrate that the SARAH domain of RASSF1A is involved in the binding to Aurora A kinase. Structural modelling confirms that a novel complex is feasible between the SARAH domain and the kinase domain of Aurora A. In addition, a regulatory role of the loop in the catalytic phosphorylation reaction has been demonstrated both experimentally and by structural modelling.
Toxicological testing of allogeneic secretome derived from peripheral mononuclear cells (APOSEC): a novel cell-free therapeutic agent in skin disease
Authors: Wuschko Silvio , Gugerell Alfred , Chabicovsky Monika , Hofbauer Helmut , Laggner Maria , Erb Michael , Ostler Tobias , Peterbauer Anja , Suessner Susanne , Demyanets Svitlana , Leuschner Jost , Moser Bernhard , Mildner Michael , Ankersmit Hendrik J. 
Published: April 2019

AbstractA cell-free approach using secretomes derived from stem cells or peripheral blood mononuclear cells is an active area of regenerative medicine that holds promise for therapies. Regulatory authorities classify these secretomes as biological medicinal products, and non- clinical safety assessment thus falls under the scope of ICH S6. A secretome of stressed peripheral blood mononuclear cells (APOSEC) was successfully tested in a toxicology program, supporting clinical use of the new drug candidate. Here, to allow for topical, dermal treatment of patients with diabetic foot ulcer, several non-clinical safety studies were performed. Acute toxicity (single dose) and neuropharmacological screening were tested intravenously in a rat model. Risk for skin sensitisation was tested in mice. A 4-week intravenous toxicity study in mice and a 4-week subcutaneous toxicity study in minipigs were conducted to cover the clinical setting and application in a rodent and a non-rodent model. Acute and repeated-dose toxicity studies show that APOSEC administered intravenously and subcutaneously does not involve major toxicities or signs of local intolerance at levels above the intended total human maximal dose of 3.3 U/kg/treatment, 200 U/wound/treatment, and 100 U/cm2/treatment. The non-clinical data support the safe topical use of APOSEC in skin diseases related to deficient wound healing.
Radial heterojunction based on single ZnO-CuxO core-shell nanowire for photodetector applications
Authors: Costas Andreea , Florica Camelia , Preda Nicoleta , Apostol Nicoleta , Kuncser Andrei , Nitescu Andrei , Enculescu Ionut 
Published: April 2019

AbstractZnO-CuxO core-shell radial heterojunction nanowire arrays were fabricated by a straightforward approach which combine two simple, cost effective and large-scale preparation methods: (i) thermal oxidation in air of a zinc foil for obtaining ZnO nanowire arrays and (ii) radio frequency magnetron sputtering for covering the surface of the ZnO nanowires with a CuxO thin film. The structural, compositional, morphological and optical properties of the high aspect ratio ZnO-CuxO core-shell nanowire arrays were investigated. Individual ZnO-CuxO core-shell nanowires were contacted with Pt electrodes by means of electron beam lithography technique, diode behaviour being demonstrated. Further it was found that these n-p radial heterojunction diodes based on single ZnO-CuxO nanowires exhibit a change in the current under UV light illumination and therefore behaving as photodetectors.
Assessment of Early Therapeutic Response to Nitroxoline in Temozolomide-Resistant Glioblastoma by Amide Proton Transfer Imaging: A Preliminary Comparative Study with Diffusion-weighted Imaging
Authors: Kumari Nisha , Thakur Nishant , Cho Hye Rim , Choi Seung Hong 
Published: April 2019

AbstractAmide proton transfer (APT) imaging is a novel molecular MRI technique to detect endogenous mobile proteins and peptides through chemical exchange saturation transfer. In this preliminary study, the purpose was to evaluate the feasibility of APT imaging in monitoring the early therapeutic response to nitroxoline (NTX) in a temozolomide (TMZ)-resistant glioblastoma multiforme (GBM) mouse model, which was compared with diffusion-weighted imaging (DWI). Here, we prepared TMZ-resistant GBM mouse model (n = 12), which were treated with 100 mg/kg/day of NTX (n = 4) or TMZ (n = 4), or saline (n = 4) for 7 days for the evaluation of short-term treatment by using APT imaging and DWI sequentially. The APT signal intensities and apparent diffusion coefficient (ADC) values were calculated and compared before and after treatment. Moreover, immunohistological analysis was also employed for the correlation between APT imaging and histopathology. The association between the APT value and Ki-67 labeling index was evaluated by using simple linear regression analysis. The short-term NTX treatment resulted in significant decrease in APT value as compared to untreated and TMZ group, in which APT signals were increased. However, we did not observe significantly increased mean ADC value following short-term NTX treatment. The Ki-67 labeling index shows a correlation with APT value. APT imaging could show the earlier response to NTX treatment as compared to ADC values in a TMZ-resistant mouse model. We believe that APT imaging can be a useful imaging biomarker for the early therapeutic evaluation in GBM patients.
Frequency and voltage response of a wind-driven fluttering triboelectric nanogenerator
Authors: Olsen Martin , Zhang Renyun , Örtegren Jonas , Andersson Henrik , Yang Ya , Olin Håkan 
Published: April 2019

AbstractTriboelectric nanogenerators (TENG:s) are used as efficient energy transducers in energy harvesting converting mechanical energy into electrical energy. Wind is an abundant source of mechanical energy but how should a good triboelectric wind harvester be designed? We have built and studied a TENG driven by air flow in a table-top sized wind tunnel. Our TENG constitutes of a plastic film of size 10 cm × 2 cm which is fluttering between two copper electrodes generating enough power to light up a battery of LED:s. We measured the voltage and frequency of fluttering at different wind speeds from zero up to 8 m/s for three electrode distances 6 mm, 10 mm and 14 mm. We found that the frequency increases linearly with the wind speed with a cutoff at some low speed. Power was generated already at 1.6 m/s. We seem to be able to explain the observed frequency dependence on wind speed by assuming excitation of the film into different harmonics in response to von Kármán vortices. We also find that the voltage increase linearly with frequency. We anticipate that TENG:s of this design could be useful both as generators and speed sensors because they work at low air speeds.
Treatment Targets for Co-Occurring Speech-Language Impairment: A Case Study
Authors: Combiths Philip N. , Barlow Jessica A. , Richard Jennifer Taps , Pruitt-Lord Sonja L. 
Published: April 2019

Purpose
The intersection of speech and language impairments is severely understudied. Despite repeatedly documented overlap and co-occurrence, treatment research for children with combined phonological and morphosyntactic deficits is limited. Especially, little is known about optimal treatment targets for combined phonological–morphosyntactic intervention. We offer a clinically focused discussion of the existing literature pertaining to interventions for children with combined deficits and present a case study exploring the utility of a complex treatment target in word-final position for co-occurring speech and language impairment.

Method
Within a school setting, a kindergarten child (aged 5;2 [years;months]) with co-occurring phonological disorder and developmental language disorder received treatment targeting a complex consonant cluster in word-final position inflected with 3rd-person singular morphology.

Results
For this child, training a complex consonant cluster in word-final position resulted in generalized learning to untreated consonants and clusters across word positions. However, morphological generalization was not demonstrated consistently across measures.

Conclusions
These preliminary findings suggest that training complex phonology in word-final position can result in generalized learning to untreated phonological targets. However, limited improvement in morphology and word-final phonology highlights the need for careful monitoring of cross-domain treatment outcomes and additional research to identify the characteristics of treatment approaches, techniques, and targets that induce cross-domain generalization learning in children with co-occurring speech-language impairment.
CMS: Physics overview
Authors: Alexakhin Vadim 
Published: April 2019

An overview of physics results from the CMS experiment at the LHC is given. The present analysis is based on data obtained for colliding proton beams at the c.m. energies of $\rad{}{s} = 8$ and 13 TeV over the period of LHC Run-1 and Run-2.New unique data on interactions of Standard Model particles at record energies were obtained in the course of the first run of LHC operation. The Higgs boson was discovered, and investigation of its properties was initiated. Measurements were performed for Standard Model processes, including rare and one previously unobserved. This allowed one to refine some parameters of the Standard Model and set limits on the parameters of some theoretical models beyond the Standard Model, for example, on the masses of new particles, the fundamental energy scales, the coupling constants, and the cross sections for the new particles production.
Hadronic properties of the photon
Authors: Gevorkyan Sergey 
Published: April 2019

Currently it is no doubt that high energy photons (real or virtual) have a hadronic component leading to photon shadowing in its interaction with nuclei. We shortly consider the difficulties appeared in the models like vector dominance model (VDM) and stress that these problems can be solved in a color dipole model inspired by Quantum Chromodynamics (QCD).
From the other hand, the color dipole model allows one to investigate the impact of vector meson polarization on their interaction with nucleons and nuclei, the challenge which is crucial for studying, for instance, such a fundamental effect as color transparency.
On the pair correlations of neutral K, D, B and Bs mesons with close momenta produced in inclusive multiparticle processes
Authors: Lyuboshitz Valery V. , Lyuboshitz Vladimir L. 
Published: April 2019

The phenomenological structure of inclusive cross-sections of the production of two neutral K mesons in hadron–hadron, hadron–nucleus and nucleus–nucleus collisions is theoretically investigated taking into account the strangeness conservation in strong and electromagnetic interactions. Relations describing the dependence of the correlations of two short-lived and two long-lived neutral kaons $K_S^0K_S^0,K_L^0K_L^0$ and the correlations of “mixed” pairs $K_S^0K_L^0$ at small relative momenta upon the space-time parameters of the generation region of K0 and K̅0 mesons have been obtained. These relations involve the contributions of Bose-statistics and S -wave strong final-state interaction of two K0 (K̅0) mesons as well as of the K0 and K̅0 mesons, and also the additional contribution of transitions K+K– → K0 K̅0, and they depend upon the relative fractions of generated pairs K0K0, K̅0 K̅0 and K0 K̅0. It is shown that under the strangeness conservation the correlation functions of the pairs $K_S^0K_S^0$ and $K_L^0K_L^0$, produced in the same inclusive process, coincide, and the difference between the correlation functions of the pairs $K_S^0K_S^0$ and $K_S^0K_L^0$ is conditioned exclusively by the production of the pairs of non-identical neutral kaons K0 K̅0.
For comparison, analogous correlations for the pairs of neutral heavy mesons D0, B0 and $B_s^0$, generated in multiple inclusive processes with charm (beauty) conservation, are also theoretically analyzed. These correlations are described by quite similar expressions: in particular, just as for K0 mesons, the correlation functions for the pairs of states with the same CP parity (RS S = RLL) and with different CP parity (RS L) do not coincide, and the difference between them is conditioned exclusively by the production of pairs D0 D̅0, B0B̅0 and $B_s^0\bar B_s^0$. However, contrary to the case of K0 mesons, here the distinction of CP-even and CP-odd states encounters difficulties – due to the insignificant differences of their lifetimes and the relatively small probability of purely CP-even and CP-odd decay channels. Nevertheless, one may hope that it will become possible at future colliders.
Study of exclusive processes within the GPD approach
Authors: Goloskokov Sergey 
Published: April 2019

In this report, we analyze the exclusive meson production in leptonhadron and hadron processes to study Generalized Parton Distributions (GPDs). It is shown that in both reactions, the photoproduction mechanism of vector meson (VM) production predominates. We estimate the VM production cross section and spin observables.
The possibility to study the exclusive VM production at NICA was analyzed. One can investigate different observables in ρ, ω and J/ψ productions to study quark and gluon GPDs. The other important process is the exclusive Drell-Yan (D-Y) production in the proton-proton reaction determined by two GPDs contributions. This reaction gives an important test of the GPD model and hopefully can be studied at NICA energies.
Der intelligente HNO-Operationssaal der Zukunft
Authors: Pirlich Markus , Stöhr Matthäus , Neumuth Thomas , Dietz Andreas 
Published: April 2019

ZusammenfassungDie zunehmende Pluralität und Komplexität technischer Assistenzsysteme stellen eine Herausforderung für den klinisch tätigen Mediziner dar. Insbesondere im OP Saal steigt der Bedarf medizintechnische Systeme und Softwarelösungen in eine gesamtheitliche Infrastruktur zu integrieren. Primäres Ziel des Aufbaus eines „digitalen (HNO-) OP Saals der Zukunft“ ist nicht nur die reine technische Verbesserung der einzelnen computergestützten Geräte und Instrumente, sondern vielmehr deren dynamische Vernetzung und Systemintegration in einem offenen modularen System. Vielversprechende wissenschaftliche Projekte gehen dabei den Fragen nach, wie die Qualität, die Sicherheit und die Anwenderfreundlichkeit technischer Systeme im Gesundheitswesen des 21. Jahrhunderts verbessert werden können. Am Beispiel der Arbeiten zu SCOT, MD PnP und OR.NET werden die verschiedenen Komponenten sichtbar, die im Gesamtkontext die Vision des HNO-Operationssaals der Zukunft erfahrbar und realistisch erscheinen lassen.
Telemedizinische Methoden in der Hals-Nasen-Ohrenheilkunde
Authors: Beule Achim 
Published: April 2019

ZUSAMMENFASSUNGTelemedizin ist ein Oberbegriff, der alle kommunikationstechnologisch unterstützte Verfahren zusammenfasst, die medizinische Leistungen über räumliche Entfernungen erbringen. Dieser rasch sich entwickelnde Bereich der Medizin besitzt gerade für das stark endoskopisch/mikroskopisch ausgerichtete Fach der Hals-Nasen-Ohrenheilkunde interessante Optionen. Für die klinische Untersuchung von Ohr, Nase, Rachen und Larynx, aber auch für die postoperative Nachsorge und die Betreuung nach Kopf-Hals-Tumor-Erkrankungen liegen Daten zum telemedizinischen Vorgehen vor, ebenso für die audiologischen Messungen nach Cochlea-Implantation. Diese Daten bieten z. T. ein interessantes Potential, z. T. weisen sie aber gerade auch auf Probleme bei unkritischer Anwendung der Telemedizin hin, deren Erfolg entscheidend von der Ausbildung des Untersuchers vor Ort abhängt. Der vorgestellte Artikel fasst die wesentlichen Erkenntnisse dieses sich sehr rasch entwickelnden Forschungsgebietes überblickartig zusammen.
Digitale Strategien in der Lehre
Authors: Dombrowski Tobias , Dazert Stefan , Volkenstein Stefan 
Published: April 2019

ZusammenfassungDie Entwicklung digitaler Strategien in der Lehre beruht auf den technologischen Fortschritten der letzten Jahrzehnte, aber auch auf der Motivation, ein didaktisches Konzept verstärkt auf den Lernenden zu zentrieren. Aus verfügbaren Daten der deutschen Hochschulmedizin ergibt sich das Bild, dass in der Medizin ganz allgemein und speziell in der HNO-Heilkunde digitale Lehrkonzepte aktuell noch eine untergeordnete Rolle spielen. Durch Erhebung eigener Daten konnten wir zeigen, dass die Studierenden in der HNO-Heilkunde in der Mehrheit auf durch den Dozenten ausgegebene Materialien als einzige Lernquelle zurückgreifen. Die Anwendung fundierter digitaler Lehrstrategien bietet daher, insbesondere fachbezogen für die HNO-Heilkunde, besondere Chancen, u. a. um dem Überangebot von Informationen aus teils unklaren Quellen im Internet entgegenzutreten.Der mögliche Digitalisierungsgrad der Lehre reicht heute von digitalen Serviceangeboten über punktuelle Angebote klassischer Lehrkonzepte und Blended Learning bis hin zum volldigitalen Studium. Die Attraktivität der curricularen Integration digitaler Lehrstrategien beruht dabei weniger auf der Nutzung rein technischer Fortschritte, sondern vielmehr auf den Möglichkeiten der Anwendung innovativer Curricula und neuer didaktischer Konzepte. In Abhängigkeit vom beabsichtigten Lehrzweck werden insbesondere dem Flipped Classroom und der digitalisierten Realität großes Potenzial zugeschrieben, während mobiles Lernen sich in der Praxis bereits individuell etabliert hat. Die Erprobung und Evaluierung digitaler Lehrinnovationen für konkrete Szenarien gehört aktuell zu den wichtigen wissenschaftlichen Herausforderungen digitaler Lehrkonzepte.Der flächendeckenden Etablierung digitaler Lehre stehen heute weniger technische Voraussetzungen, sondern vielmehr fehlende Finanzierungsmöglichkeiten im Wege, da Förderungen derzeit überwiegend projektgebunden stattfinden, bei dauerhafter Umsetzung jedoch regelmäßige Kosten vorhanden sind. Zur Unterstützung dieser vielversprechenden Lehrformen könnte die Förderung der Einrichtung von Abteilungen für digitale Lehre mit Bereitstellung von entsprechenden Hard- und Softwarelösungen an den Universitäten wesentlich beitragen. Durch die Bildung von Kooperationen zur Nutzung solcher digitalen Plattformen ergäbe sich eine hohe Effizienz in der Verbreitung unter gleichzeitiger Ausnutzung von Sparpotenzialen.
Register – Auswertung von multizentrischen Daten
Authors: Schraven Sebastian , Mlynski Robert 
Published: April 2019

ZusammenfassungEin Register dokumentiert aktiv und standardisiert Patientendaten zu vorab definierten Fragestellungen. Der Begriff „Register“ (lat. regerere – „eintragen“) hebt den datenhaltenden Aspekt hervor mit dem Ziel epidemiologische Zusammenhänge und Unterschiede zu beschreiben, die Qualitätssicherung und -verbesserung sowie die klinische Forschung zu unterstützen. Die Evaluation der Wirksamkeit in der Versorgungsroutine, das Monitoren der Patientensicherheit sowie die ökonomische Evaluation und die Mindestmengenforschung sind weitere Aufgaben von Registern. Patienten und meldende Einrichtungen bestimmen maßgeblich die Registerqualität durch Vollständigkeit, Vollzähligkeit und hohe Datenvalidität. Dies ist bei der Konzeption, der Finanzierung und dem Betrieb eines Registers zu berücksichtigen. Bei der Auswertung der multizentrischen Daten des Registers kommt der Analyse von potenziell konfundierender oder Effekt modifizierender Variablen eine hohe Bedeutung zu. Regelmäßiges Feedback an die Meldeeinrichtungen, Patienteninformationen, öffentliche Mitteilungen sowie wissenschaftliche Publikationen erhöhen ebenso wie die Einhaltung des Datenschutzes die Transparenz des Registers. Die Hals-, Nasen- und Ohrenheilkunde hat bis auf die Integration in das Krebsregister und dem sich im Aufbau befindenden Neugeborenen Hörscreening-Register wenig Berührungspunkte mit Registern. Die Vielzahl messbarer Parameter in den einzelnen Schwerpunkten des Fachgebiets, wie z. B. der Otologie, Phoniatrie, Rhinologie, Allergologie, usw., stellt die Grundlage für verschiedene potenzielle Register dar. Klinische Fragestellungen, Präventionsmaßnahmen, Qualitätssicherung, Versorgungsforschung und Empfehlungen für die Gesundheitspolitik wären wissenschaftlich fundiert und evidenzbasiert.
Topographic mapping of a specimen after endoscopic submucosal dissection
Authors: Ebigbo Alanna , Probst Andreas , Messmann Helmut , Märkl Bruno , Nam-Apostolopoulos Yun-Chung 
Published: April 2019

Abstract
Background and study aims Mapping of pathologic specimens after endoscopic submucosal dissection (ESD) is common practice in Asian countries, especially in Japan. However, there is a lack of awareness for this technique in Europe. In this report, we demonstrate the feasibility and benefits of topographic mapping in a Western setting.
Contrast-enhanced endoscopic ultrasound for the differential diagnosis between benign and malignant lymph nodes: a meta-analysis
Authors: Lisotti Andrea , Ricci Claudio , Serrani Marta , Calvanese Claudio , Sferrazza Sandro , Brighi Nicole , Casadei Riccardo , Fusaroli Pietro 
Published: April 2019

Abstract
Background The differential diagnosis between benign and malignant lymph nodes (LNs) is crucial for patient management and clinical outcome. The use of contrast-enhanced endoscopic ultrasound (EUS) has been evaluated in several studies with diverse results. The aim of this meta-analysis was to evaluate the pooled diagnostic accuracy of contrast-enhanced EUS (CE-EUS) and contrast-enhanced harmonic EUS (CH-EUS) in this setting.
Methods A systematic electronic search was performed, including all original papers dealing with assessment of the nature of the LNs using CE-EUS or CH-EUS. A meta-analysis was performed to obtain pooled sensitivity, specificity, positive and negative likelihood ratio, and diagnostic odds ratio. The Summary Receiver Operating Characteristic (ROC) Curve method was used to calculate the area under the curve. Statistical analysis was carried out using Meta-Disc V.1.4, Stata V.12.0 and Review Manager V.5.2.
Results Among 210 pertinent studies, four (336 patients) were included in the analysis. The pooled sensitivity was 82.1 % (75.1 – 87.7 %) and pooled specificity was 90.7 % (85.9 – 94.3 %) with significant heterogeneity found in sensitivity; the positive-likelihood ratio (LR) was 7.77 (5.09 – 11.85) and the negative-LR was 0.15 (0.05 – 0.46); the pooled diagnostic odds ratio (DOR) was 54 (15 – 190). Subgroup analysis including studies performed using CH-EUS (two studies, 177 LNs) showed a pooled sensitivity of 87.7 % (77.0 – 93.9 %) and a pooled specificity of 91.8 % (84.5 % – 96.4 %) with no significant heterogeneity; the pooled positive-LR was 9.51 (4.95 – 18.28) and the pooled negative-LR was 0.14 (0.06 – 0.35); pooled DOR was 68.42 (15.5 – 301.4).
Conclusions From these data, CE-EUS is not recommended due to inadequate sensitivity. On the other hand, CH-EUS studies showed optimal accuracy (pooled sensitivity 87.7 % and specificity 91.8 %), comparable to elastography and even EUS-guided fine needle aspiration (EUS-FNA), suggesting a role in the diagnostic algorithm.