bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of vitamin D supplementation on the glycemic control of pre-diabetic Qatari patients in a randomized control trial
Authors: Al Thani Mohammed , Sadoun Eman , Sofroniou Angeliki , Jayyousi Amin , Baagar Khaled Ahmed Mohamed , Al Hammaq Abdulla , Vinodson Benjamin , Akram Hammad , Bhatti Zaid Shakoor , Nasser Heba Samir , Leventakou Vasiliki 
Published: October 2019

Abstract

Background
Vitamin D deficiency is associated with indicators of pre-diabetes including, insulin resistance, β-cell dysfunction and elevated plasma glucose with controversial findings from current trials. This study aims to investigate the long-term effect of vitamin D on glucose metabolism and insulin sensitivity in pre-diabetic and highly vitamin-deficient subjects.

Methods
One hundred thirty-two participants were randomized to 30,000 IU vitamin D weekly for 6 months. Participants underwent oral glucose tolerance test (OGTT) at 3-month intervals to determine the change in plasma glucose concentration at 2 h after 75 g OGTT (2hPCG). Secondary measurements included glycated hemoglobin, fasting plasma glucose and insulin, post-prandial insulin, indices of insulin sensitivity (HOMA-IR, Matsuda Index), β-cell function (HOMA-β, glucose and insulin area under the curve (AUC), disposition and insulinogenic indices), and lipid profile.

Results
A total of 57 (vitamin D) and 75 (placebo) subjects completed the study. Mean baseline serum 25(OH) D levels were 17.0 ng/ml and 14.9 ng/ml for placebo and vitamin D group, respectively. No significant differences were observed for 2hPC glucose or insulin sensitivity indices between groups. HOMA-β significantly decreased in the vitamin D group, while area under curve for glucose and insulin showed a significant reduction in β-cell function in both groups. Additionally, HOMA-β was found to be significantly different between control and treatment group and significance persisted after adjusting for confounding factors.

Conclusion
Vitamin D supplementation in a pre-diabetic and severely vitamin-deficient population had no effect on glucose tolerance or insulin sensitivity. The observed reduction in β-cell function in both placebo and vitamin D groups could be attributed to factors other than supplementation.

Trial registration
NCT02098980, 28/03/2014 (www.clinicaltrials.gov).
Adapting and implementing Caring Contacts in a Department of Veterans Affairs emergency department: a pilot study protocol
Authors: Landes Sara J. , Kirchner JoAnn E. , Areno John P. , Reger Mark A. , Abraham Traci H. , Pitcock Jeffery A. , Bollinger Mary J. , Comtois Katherine Anne 
Published: October 2019

AbstractBackgroundSuicide among veterans is a problem nationally, and suicide prevention remains a high priority for the Department of Veterans Affairs (VA). Focusing suicide prevention initiatives in the emergency department setting provides reach to veterans who may not be seen in mental health and targets a critical risk period, transitions in care following discharge. Caring Contacts is a simple and efficacious suicide prevention approach that could be used to target this risk period. The purpose of this study is to (1) adapt Caring Contacts for use in a VA emergency department, (2) conduct a pilot program at a single VA emergency department, and (3) create an implementation toolkit to facilitate spread of Caring Contacts to other VA facilities.MethodsThis project includes planning activities and a pilot at a VA emergency department. Planning activities will include determining available data sources, determining logistics for identifying and sending Caring Contacts, and creating an implementation toolkit. We will conduct qualitative interviews with emergency department staff and other key stakeholders to gather data on what is needed to adapt and implement Caring Contacts in a VA emergency department setting and possible barriers to and facilitators of implementation. An advisory board of key stakeholders in the facility will be created. Qualitative findings from interviews will be presented to the advisory board for discussion, and the board will use these data to inform decision making regarding implementation of the pilot. Once the pilot is underway, the advisory board will convene again to discuss ongoing progress and determine if any changes are needed to the implementation of the Caring Contacts intervention.DiscussionFindings from the current project will inform future scale-up and spread of this innovation to other VA medical center emergency departments across the network and other networks. The current pilot will adapt Caring Contacts, create an implementation toolkit and implementation guide, evaluate the feasibility of gathering outcome measures, and provide information about what is needed to implement this evidence-based suicide prevention intervention in a VA emergency department.
A nomogram predicting the recurrence of hepatocellular carcinoma in patients after laparoscopic hepatectomy
Authors: Pan Yang‐Xun , Chen Jian‐Cong , Fang Ai‐Ping , Wang Xiao‐Hui , Chen Jin‐Bin , Wang Jun‐Cheng , He Wei , Fu Yi‐Zhen , Xu Li , Chen Min‐Shan , Zhang Yao‐Jun , Li Qi‐Jiong , Zhou Zhong‐Guo 
Published: October 2019

AbstractBackgroundPatients with hepatocellular carcinoma (HCC) undergoing surgical resection still have a high 5‐year recurrence rate (~ 60%). With the development of laparoscopic hepatectomy (LH), few studies have compared the efficacy between LH and traditional surgical approach on HCC. The objective of this study was to establish a nomogram to evaluate the risk of recurrence in HCC patients who underwent LH.MethodsThe clinical data of 432 patients, pathologically diagnosed with HCC, underwent LH as initial treatment and had surgical margin > 1 cm were collected. The significance of their clinicopathological features to recurrence‐free survival (RFS) was assessed, based on which a nomogram was constructed using a training cohort (n = 324) and was internally validated using a temporal validation cohort (n = 108).ResultsHepatitis B surface antigen (hazard ratio [HR], 1.838; P= 0.044), tumor number (HR, 1.774; P= 0.003), tumor thrombus (HR, 2.356; P= 0.003), cancer cell differentiation (HR, 0.745; P= 0.080), and microvascular tumor invasion (HR, 1.673; P =0.007) were found to be independent risk factors for RFS in the training cohort, and were used for constructing the nomogram. The C‐index for RFS prediction in the training cohort using the nomogram was 0.786, which was higher than that of the 8th edition of the American Joint Committee on Cancer TNM classification (C‐index, 0.698) and the Barcelona Clinic Liver Cancer staging system (C‐index, 0.632). A high consistency between the nomogram prediction and actual observation was also demonstrated by a calibration curve. An improved predictive benefit in RFS and higher threshold probability of the nomogram were determined by receiver operating characteristic curve analysis, which was also confirmed in the validation cohort compared to other systems.ConclusionsWe constructed and validated a nomogram able to quantify the risk of recurrence after initial LH for HCC patients, which can be clinically implemented in assisting the planification of individual postoperative surveillance protocols.
The Brazilian Society of Rheumatology recommendations on investigation and diagnosis of systemic autoimmune myopathies
Authors: de Souza Fernando Henrique Carlos , de Araújo Daniel Brito , Vilela Verônica Silva , Simões Ricardo Santos , Bernardo Wanderley Marques , Frank Thais Amanda , da Cunha Bernardo Matos , Shinjo Samuel Katsuyuki 
Published: October 2019

AbstractBackgroundThis research is recommended by the Myopathy Committee of the Brazilian Society of Rheumatology for the investigation and diagnosis of systemic autoimmune myopathies.BodyA systematic literature review was performed in the Embase, Medline (PubMed) and Cochrane databases, including studies published until October 2018. PRISMA was used for the review, and the articles were evaluated, based on the Oxford levels of evidence. Ten recommendations were developed addressing different aspects of systemic autoimmune myopathy investigation and diagnosis.ConclusionsThe European League Against Rheumatism/ American College of Rheumatology (EULAR/ACR) classification stands out for the diagnosis of systemic autoimmune myopathies. Muscular biopsy is essential, aided by muscular magnetic resonance images and electroneuromyography in complementary research. Analysis of the factors related to prognosis with the evaluation of extramuscular manifestations, and comorbidities and intense investigation regarding differential diagnoses are mandatory.
Management of clozapine-induced sialorrhoea
Authors: Gupta Sumeet , Khastgir Udayan , Croft Matthew , Roshny Sefat 
Published: October 2019

SUMMARYSialorrhoea (hypersalivation) is a common adverse effect of clozapine. If severe, it can affect patients' quality of life and adherence to the treatment. Clinicians therefore need to proactively manage this effect. At present, no drugs are licensed to manage clozapine-induced sialorrhoea, although there are many off-label treatment options, with variable effectiveness. Anticholinergic medications are commonly prescribed for it, but they have limited effect and can worsen constipation. This article gives a brief overview of other practical and pharmacological management options.
Inflammatory Stimuli Trigger Increased Androgen Production and Shifts in Gene Expression in Theca-Interstitial Cells
Authors: Fox Chelsea W , Zhang Lingzhi , Sohni Abhishek , Doblado Manuel , Wilkinson Miles F , Chang R Jeffrey , Duleba Antoni J 
Published: October 2019

AbstractPolycystic ovary syndrome (PCOS) is a common reproductive endocrine disorder characterized by theca cell hyperplasia and excessive androgen production. An increasing body of evidence has pointed to a close association between PCOS and low-grade chronic systemic inflammation. However, the mechanistic basis for this linkage is unknown. Therefore, we evaluated the effects of the inflammatory agents lipopolysaccharide (LPS) and IL-1β on rat theca-interstitial cells (TICs). We found that incubation with either LPS or IL-1β elicited a dose-dependent increase in both TIC viability and androgen production. Using RNA sequencing analysis, we found that both of these inflammatory agents also triggered profound and widespread shifts in gene expression. Using a stringent statistical cutoff, LPS and IL-1β elicited differential expression of 5201 and 5953 genes, respectively. Among the genes upregulated by both LPS and IL-1β were key regulatory genes involved in the cholesterol and androgen biosynthesis pathways, including Cyp17a1, Cyp11a1, Hsd3b, and Hmgcr. This provides a molecular explanation for the mechanism of action of inflammatory agents leading to increased androgen production. Gene ontology and pathway analysis revealed that both LPS and IL-1β regulated genes highly enriched for many common functions, including the immune response and apoptosis. However, a large number of genes (n = 2222) were also uniquely regulated by LPS and IL-1β, indicating that these inflammatory mediators have substantial differences in their mechanism of action. Together, these findings highlight the potential molecular mechanisms through which chronic low-grade inflammation contributes to the pathogenesis of androgen excess in PCOS.
Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1
Authors: Papini Diana , Fant Xavier , Ogawa Hiromi , Desban Nathalie , Samejima Kumiko , Feizbakhsh Omid , Askin Bilge , Ly Tony , Earnshaw William C. , Ruchaud Sandrine 
Published: October 2019

ABSTRACT
Timely and precise control of Aurora B kinase, the chromosomal passenger complex (CPC) catalytic subunit, is essential for accurate chromosome segregation and cytokinesis. Post-translational modifications of CPC subunits are directly involved in controlling Aurora B activity. Here, we identified a highly conserved acidic STD-rich motif of INCENP that is phosphorylated during mitosis in vivo and by Plk1 in vitro and is involved in controlling Aurora B activity. By using an INCENP conditional-knockout cell line, we show that impairing the phosphorylation status of this region disrupts chromosome congression and induces cytokinesis failure. In contrast, mimicking constitutive phosphorylation not only rescues cytokinesis but also induces ectopic furrows and contractile ring formation in a Plk1- and ROCK1-dependent manner independent of cell cycle and microtubule status. Our experiments identify the phospho-regulation of the INCENP STD motif as a novel mechanism that is key for chromosome alignment and cytokinesis.
This article has an associated First Person interview with the first author of the paper.
The Relationship Between Tumor Budding, Tumor Microenvironment, and Survival in Patients with Primary Operable Colorectal Cancer
Authors: van Wyk Hester C. , Roseweir Antonia , Alexander Peter , Park James H. , Horgan Paul G. , McMillan Donald C. , Edwards Joanne 
Published: October 2019

AbstractBackgroundTumor budding is an independent prognostic factor in colorectal cancer (CRC) and has recently been well-defined by the International Tumour Budding Consensus Conference (ITBCC).ObjectiveThe aim of the present study was to use the ITBCC budding evaluation method to examine the relationship between tumor budding, tumor factors, tumor microenvironment, and survival in patients with primary operable CRC.MethodsHematoxylin and eosin-stained slides of 952 CRC patients diagnosed between 1997 and 2007 were evaluated for tumor budding according to the ITBCC criteria. The tumor microenvironment was evaluated using tumor stroma percentage (TSP) and Klintrup–Makinen (KM) grade to assess the tumor inflammatory cell infiltrate.ResultsHigh budding (n = 268, 28%) was significantly associated with TNM stage (p < 0.001), competent mismatch repair (MMR;p < 0.05), venous invasion (p < 0.001), weak KM grade (p < 0.001), high TSP (p < 0.001), and reduced cancer-specific survival (CSS) (hazard ratio 8.68, 95% confidence interval 6.30–11.97;p < 0.001). Tumor budding effectively stratifies CSS stage T1 through to T4 (allp < 0.05) independent of associated factors.ConclusionsTumor budding effectively stratifies patients’ survival in primary operable CRC independent of other phenotypic features. In particular, the combination of T stage and budding should form the basis of a new staging system for primary operable CRC.
Exploring the impact of glutathione S-transferase (GST)-based metabolic resistance to insecticide on vector competence of Anopheles funestus for Plasmodium falciparum
Authors: Ndo Cyrille , Kopya Edmond , Irving Helen , Wondji Charles 
Published: October 2019

Background:Malaria control heavily relies on insecticide-based interventions against mosquito vectors. However, the increasing spread of insecticide resistance is a major threat. The extent to which such resistance, notably metabolic resistance, influences the development of thePlasmodiumparasite and its impact on overall malaria transmission remains poorly characterized. Here, we investigated whether glutathione S-transferase-based resistance could influencePlasmodium falciparumdevelopment inAnopheles funestus.Methods:Anopheles funestusfemales were infected withP. falciparumgametocytes and midguts were dissected at day 7 post infection for detection/quantification of oocysts. Infection parameters were compared between individuals with different L119F-GSTe2 genotypes, and the polymorphism of the GSTe2 gene was analyzed in infected and uninfected mosquito groups.Results:Overall, 403An. funestus mosquitoes were dissected and genotyped. The frequency of the L119F-GSTe2 resistance allele was significantly higher in non-infected (55.88%) compared to infected (40.99%) mosquitoes (Fisher's exact test, P<0.0001). Prevalence of infection was significantly higher in heterozygous and homozygous susceptible genotypes (P<0.001). However, homozygous resistant and heterozygous mosquitoes exhibited significantly higher infection intensity (P<0.01). No association was observed between the GSTe2 polymorphism and the infection status of mosquitoes.Conclusion:Altogether, these results suggest that GSTe2-based metabolic resistance may affect the vectorial competence of resistantAn. funestusmosquitoes toP. falciparuminfection, by possibly increasing its permissiveness toPlasmodiuminfection.
EDUCAÇÃO EM DIREITOS HUMANOS E AS QUESTÕES DE GÊNERO NA ESCOLA
Authors: Santos Ana Paula Da Silva , Brito Leandro Teófilo de 
Published: October 2019

A questão dos direitos humanos tem se apresentado como uma problemática de difícil compreensão nos dias atuais, assim como o desafio do reconhecimento das diferenças. Mesmo com a construção de documentos nacionais e internacionais centrados na perspectiva dos direitos humanos, ainda convivemos com fortes violações, múltiplas violências, desigualdades sociais e diversas formas de preconceitos e discriminações no cotidiano das relações sociais. Neste sentido, não podemos deixar de pensar nas questões que envolvem as relações de gênero e as desigualdades sofridas por meninas e mulheres, em diversos espaços sociais, sobretudo na escola. O presente estudo visa apresentar considerações sobre a educação em direitos humanos e as questões de gênero no contexto escolar, utilizando para tanto a perspectiva intercultural. Esta perspectiva nos coloca no âmbito de compreender a educação em direitos humanos como uma mediação para a construção de uma sociedade inclusiva, igualitária e plural onde os sujeitos possam vivenciar experiências que sejam permeadas pelos direitos humanos e, que, se tornem sujeitos de direito.
Hubungan panjang-berat ikan monacanthus cinensis dan acreichthys tomentosus di Pulau Fair, Tual, Maluku Tenggara
Authors: Kumayanjati Bayu , Triandiza Teddy , Kusnadi Agus 
Published: October 2019

Abstract. Length-weight relationship analysis is used to determined the pattern of the growth for the fish. Based on this knowledge, field study have been done at the Fair island waters, the district of Tual, in April, June and August 2014, to collect data length and weight of Monacanthus cinensis and Acreichtys tomentosus. The length of the fish is measured using a digital caliper with an accuracy of 0.01 mm and weighed using an analytical balance precision balance ACS - AD 300 with an accuracy of 0.01 g. The number of fish samples analyzed were 288 for M. cinensis and 270 for A. tomentosus, respectively. Based on the analysis of length-weight relationship and condition factor, M.cinensis and A.tomentosus have the same growth pattern, i.e., negative allometric, with b values 2.505 and 2.3195 (b 3), respectively.Value of condition factor were 1.083 - 3.379 for M. cinensis and 1.003 - 4.106 for A. tomentosus, both fish were included in the category of fish that have a slightly flattened body.Keywords: Monacanthus cinensis, Acreichthys tomentosus, length-weight relationship, growth pattern, Fair Island Abstrak. Analisa hubungan panjang berat ikan merupakan analisa untuk mengetahui pola pertumbuhan ikan. Adapun penelitian ini dilakukan pada ikan Monacanthus cinensis dan Acreichthys tomentosus pada bulan April, Juni dan Agustus 2014 di perairan Pulau Fair, Tual, Maluku Tenggara. Ikan hasil tangkapan diukur panjangnya menggunakan digital caliper dengan ketelitian 0,01 mm, dan beratnya menggunakan timbangan analitik ACS precision balance-AD 300 dengan ketelitian 0,01 g. Jumlah ikan sampel yang dianalisa masing-masing sebanyak 288 ekor untuk M. cinensis dan 270 ekor untuk A. tomentosus. Berdasarkan analisa hubungan panjang berat dan faktor kondisi, M. cinensis dan A. tomentosus memiliki pola pertumbuhan yang sama yaitu alometrik negatif dengan nilai b masing-masing sebesar 2,505 dan 2,3195 (b3). Nilai faktor kondisi (Kr) untuk kedua jenis ikan tersebut masing-masing berkisar antara 1,083 – 3,379 untuk ikan M. cinensis dan 1,003 – 4,106 untuk A. tomentosus, keduanya termasuk dalam kelompok ikan yang memiliki bentuk badan yang agak pipih.Kata Kunci: Monacanthus cinensis, Acreichthys tomentosus, hubungan panjang berat, pola pertumbuhan, Pulau Fair
Vivencia del cuidado de enfermería en un proceso de duelo.
Authors: Hernandez Sampayo Luzmila 
Published: October 2019

La muerte perinatal desde el ámbito institucional es un hecho de difícil confrontación tanto para los padres quienes experimentan la pérdida, como para el personal de salud que presencia el evento.La teoría de Kristen Swanson, considera que la meta de la enfermería es brindar cuidado a la mujer y su núcleo familiar, de manera significativa para ayudarla en la aceptación de la muerte de un ser querido. El apoyo que el personal de salud brinda a los padres y la familia, es significativa en la aceptación de la pérdida y la elaboración del duelo.El presente artículo relata la experiencia de una enfermera en su interacción con una mujer que afronto la muerte de su hija al momento de nacer. Se analiza con base en la teoría de los cuidados de Kristen Swanson y se centra en los elementos metaparadigmáticos y los procesos o categorías del cuidado de enfermería.
ATIVIDADE NEUROPROTETORA DA MINOCICLINA NA ISQUEMIA CEREBRAL: REVISÃO SISTEMÁTICA
Authors: Alencar Jean Pierre De Oliveira , Alencar Gustavo De Oliveira , Lima Iri Sandro Pampolha 
Published: October 2019

O acidente vascular cerebral é causado pela interrupção do fluxo sanguíneo cerebral, podendo ser de gênese isquêmica. Os subtipos são determinados conforme a origem, podendo ser: aterosclerose de grandes artérias, cardioembolismo, oclusão de pequenas artérias, de outras origens determinadas e de origem desconhecida. O tratamento farmacológico é o Ativador de Plasminogenio Tecidual, de estreita janela terapêutica. A minociclina é capaz de atravessar a barreira hematoencefálica, atuando na perda neuronal, sendo um potencial neuroprotetor de grande aplicabilidade e especificidade, bloqueando grupos celulares responsáveis pelo processo inflamatório e degenerativo. O objetivo foi realizar uma revisão sistemática de caráter qualitativo. As bases de dados consultadas foram: MEDLINE), PubMed e SciELO, com os descritores: minociclina/neuroproteção e minocycline/neuroprotection, Apenas sete estudos foram selecionados por meio do protocolo de pesquisa, publicados entre 2014 e 2018 e disponíveis na íntegra gratuitamente. Minociclina possui relação com a inibição de NF-κB nos neurônios, efeito antioxidante, antiapoptótico, melhora no dano tecidual, recuperação funcional em animais, envolvimento com MCP1P1 e correlação com fatores os fatores CREB, pCREB, e BDNF. É notável a capacidade de neuroproteção obtida por meio do tratamento com minociclina em modelos isquêmicos.