bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-ionic surfactant vesicles exert anti-inflammatory effects through inhibition of NFκB
Authors: McGahon Jonathan , Woods Stuart , D’Elia Riccardo , Roberts Craig W. 
Published: November 2024

AbstractInflammation can be an unwanted consequence or cause of debilitating diseases of infectious and non-infectious aetiologies. Current anti-inflammatory medications have several deficiencies including lack of specificity and undesirable side effects. Herein, the potential of non-ionic surfactant vesicles (NISV) comprised of monopalmityol glycerol, dicetyl phosphate and cholesterol) as an anti-inflammatory drug and their mode of action is investigated. NISV were able to inhibit LPS-induced IL-6 from BMD macrophages. The individual components of NISV, monopalmityol glycerol, dicetyl phosphate and cholesterol did not affect LPS induced IL-6 levels, proving that formulation of NISV is essential for their anti-inflammatory effects. Transcriptomic analyses showed NISV mediated down-regulation of transcripts for inflammatory mediators in LPS stimulated macrophages. Notably, NISV downregulate NF-κB transcripts in LPS stimulated macrophages. Measurement of inflammatory mediators by cytometric bead array validated a number of transcriptomic findings as NISV were found to inhibit LPS induced IL-6, IL-12, and multiple chemokines. Further investigation demonstrated that NISV inhibited Poly(I:C) or Pam3csk4 induced inflammatory mediators. This indicates that the effects of NISV are distal to both MyD88 and TRIF signalling. Overall, the data generated highlights the potential of NISV as an anti-inflammatory therapeutic.
Genome-aware annotation of CRISPR guides validates targets in variant cell lines and enhances discovery in screens
Authors: Lam Simon , Thomas John C. , Jackson Stephen P. 
Published: November 2024

Abstract
Background
CRISPR-Cas9 technology has revolutionised genetic screens and can inform on gene essentiality and chemo-genetic interactions. It is easily deployed and widely supported with many pooled CRISPR libraries available commercially. However, discrepancies between the reference genomes used in the design of those CRISPR libraries and the cell line under investigation can lead to loss of signal or introduction of bias. The problem is particularly acute when dealing with variant cell lines such as cancer cell lines.

Results
Here, we present an algorithm, EXOme-guided Re-annotation of nuCleotIde SEquences (Exorcise), which uses sequence search to detect and correct mis-annotations in CRISPR libraries. Exorcise verifies the presence of CRISPR targets in the target genome and applies corrections to CRISPR libraries using existing exome annotations. We applied Exorcise to re-annotate guides in pooled CRISPR libraries available on Addgene and found that libraries designed on a more permissive reference sequence had more mis-annotations. In simulated CRISPR screens, we modelled common mis-annotations and found that they adversely affect discovery of hits in the intermediate range. We then confirmed this by applying Exorcise on datasets from Dependency Map (DepMap) and the DNA Damage Response CRISPR Screen Viewer (DDRcs), where we found improved discovery power upon Exorcise while retaining the strongest hits.

Conclusions
Pooled CRISPR libraries map guide sequences to genes and these mappings might not be ready to use due to permissive library design or investigating a variant cell line. By re-annotating CRISPR guides, Exorcise focuses CRISPR experiments towards the genome of the cell line under investigation. Exorcise can be applied at the library design stage or the analysis stage and allows post hoc re-analysis of completed screens. It is available under a Creative Commons Zero v1.0 Universal licence at https://github.com/SimonLammmm/exorcise.
VExUS: common misconceptions, clinical use and future directions
Authors: Rola Philippe , Haycock Korbin , Spiegel Rory , Beaubien-Souligny William , Denault Andre 
Published: November 2024

AbstractThere has been a significant interest in venous congestion in recent years, among which the VExUS score has been prominent, both in clinical practice and research efforts. We have noted some recurrent misconceptions among clinicians which are also reflected in certain research efforts. Notably, the misguided attempt to correlate VExUS to volume status, which is only one of the factors influencing it, as well as attempts to re-interpret VExUS in the context of certain pathologies, which reflects a fundamental misunderstanding of its circulatory perspective. In this article we review the physiological basis of the VExUS assessment as a measure and marker of venous congestion from the organs’ standpoint and its role as part of the emerging concept of fluid tolerance, in hopes to address these misconceptions for clinicians and for important further studies.
Associations between maternal personality dysfunction and emotion suppression and adolescent emotion suppression
Authors: Phillips Jennifer J. , Smith Cynthia L. , Bell Martha Ann 
Published: November 2024

Abstract
Background
Adaptive strategies of emotion regulation are important for adolescents, as maladaptive strategies of such can manifest as psychopathology that is sometimes severe. Individual biological characteristics and influences from peers have been shown to have an effect on the development of emotion regulation strategies in adolescents. Maternal factors, however, have received less attention in this age group regarding how they might predict emotion regulation in adolescents. Given that prior work has demonstrated that certain maternal factors, like emotion regulation and personality, play a crucial role in the development of emotion regulation strategies in early childhood, we sought to examine these associations in adolescents in our current study.

Methods
Adolescents and their mothers (n = 123) both self-reported data on their own emotion regulation, and mothers also self-reported data on their own personality dysfunction. We operationalized maternal and adolescent emotion regulation as emotion suppression, a maladaptive emotion regulation strategy that is commonly used by adolescents.

Results
Our data demonstrated that both maternal emotion suppression and interpersonal personality dysfunction were positively associated with adolescent emotion suppression. No associations among maternal intrapersonal personality functioning and adolescent emotion suppression were detected.

Conclusions
Maternal personality dysfunction and emotion suppression both independently predicted adolescent emotion suppression use. These results support the idea that maternal characteristics play a role in shaping emotion regulation in adolescence.
Influence of wood modification on parameter settings and treatment results in CO2 laser structuring of beech veneers
Authors: Doğan Güneş Akın , Wascher Richard , Avramidis Georg , Viöl Wolfgang , Gerhard Christoph 
Published: November 2024

AbstractIn this study, the possible influences of thermal modification of wood on the quality of laser texturing of beech veneers are investigated by comparing native and thermally modified samples. By varying the process parameters of a CO2 laser, the surfaces of both types of veneer were textured and the resulting surface roughness and aspect ratios were analyzed in order to evaluate the efficiency of the laser texturing and the quality of the textures produced. The main results show that the thermal modification of the wood influences the cutting widths, the removal depths, and the surface roughness, with thermally modified veneers generally having larger cutting widths and different removal depths compared to native veneers, indicating the influence of the wood modifications on the material physical and chemical properties and their interaction with the laser processing. Furthermore, the study shows how the laser processing parameters—feed rate and laser power—influence the surface quality and structural dimensions of the engraved lines, and establishes that the moisture content of the wood has a significant influence on its thermal conductivity and thus on the laser cutting process. The research work highlights the complexity of laser texturing of wood and emphasizes the need to take into account the change in the intrinsic properties of the material as a result of thermal modification.
Preclinical evaluation and automated synthesis of [89Zr]ZrDFOSquaramide-girentuximab for diagnostic imaging of carbonic anhydrase IX positive tumours
Authors: Noor Asif , McGowan Emily R. , Van Zuylekom Jessica K. , Cullinane Carleen , Roselt Peter D. , Hicks Rodney J. , Wheatcroft Michael P. , Donnelly Paul S. 
Published: November 2024

Abstract
Background
Carbonic Anhydrase IX (CAIX) is a zinc metalloenzyme that is over-expressed in many cancers making it a valid target for targeted diagnostic imaging with Positron Emission Tomography (PET). The monoclonal antibody girentuximab binds to CAIX and when radiolabelled with positron-emitting zirconium-89 can be used for diagnostic PET imaging of CAIX positive tumours.

Results
Reaction of desferrioxamine squaramide ethyl ester with girentuximab allowed isolation of a conjugate with desferrioxamine squaramide (DFOSq) covalently attached to girentuximab through stable vinylogous amide linkages to give DFOSq-girentuximab. This conjugate was radiolabelled with zirconium-89 to give [89Zr]ZrDFOSq-girentuximab and the tumour uptake of the tracer was evaluated in CAIX positive HT29 tumour-bearing mice. Analysis of the PET images and biodistribution studies showed that the tracer displays high tumour uptake. An automated process for production of [89Zr]ZrDFOSq-girentuximab was developed, using [89Zr]ZrCl4 as a starting material that was also synthesized in an automated process. This automated process allows isolation of [89Zr]ZrDFOSq-girentuximab in radiochemical yields of 80–90% and in > 95% radiochemical purity.

Conclusions
[89Zr]ZrDFOSq-girentuximab has high uptake in CAIX positive tumours. An automated procedure for the synthesis of [89Zr]ZrDFOSq-girentuximab using [89Zr]ZrCl4 as a starting material has been developed. This automated process could be readily adapted to other antibodies.
Endovascular treatment of lower limb acute DVT: current trends and future directions
Authors: Siciliano Francesco , Ronconi Edoardo , Rossi Tommaso , Fanelli Federica , Krokidis Miltiadis , Sirignano Pasqualino , Rossi Michele , Tipaldi Marcello Andrea 
Published: November 2024

Abstract
Aim of the study
This systematic review aims to evaluate the efficacy, safety, and comparative outcomes of endovascular treatments for acute lower limb deep vein thrombosis (DVT), including catheter-directed thrombolysis (CDT), pharmacomechanical thrombectomy (PMT), mechanical thrombectomy, and venous stenting, drawing insights from a diverse range of studies.

Materials and methods
A comprehensive literature search identified 33 relevant studies, including randomized controlled trials, cohort studies, systematic reviews, and case reports. Data extraction focused on study design, intervention type, outcome measures, and follow-up duration.

Results
Catheter-directed thrombolysis demonstrates promising results in enhancing venous patency and reducing post-thrombotic syndrome, with careful patient selection being crucial. Pharmacomechanical and mechanical thrombectomy devices offer immediate and long-term benefits, emphasizing individualized patient care. Venous stenting serves as a crucial adjunctive therapy, particularly in cases of residual venous obstruction, though further research is needed for optimal patient selection and long-term outcomes. Timing and selection of endovascular interventions remain critical considerations, necessitating multidisciplinary approaches and ongoing research.

Conclusion
This review provides valuable insights for clinicians and researchers, guiding evidence-based decision-making and shaping future research directions in the dynamic field of endovascular interventions for acute lower limb DVT.
Cheatgrass alters flammability of native perennial grasses in laboratory combustion experiments
Authors: Harrison Georgia R. , Jones Lisa C. , Ellsworth Lisa M. , Strand Eva K. , Prather Timothy S. 
Published: November 2024

Abstract
Background
The invasive annual grass cheatgrass (Bromus tectorum) increases fuel continuity, alters patterns of fire spread, and changes plant communities in sagebrush shrublands of the Great Basin (USA) and adjacent sagebrush steppe, but no studies have contrasted its flammability to native perennial grasses. Understanding cheatgrass flammability is crucial for predicting fire behavior, informing management decisions, and assessing fire risk in invaded areas. This study aimed to determine the flammability of cheatgrass compared to two native perennial grasses (Columbia needlegrass [Achnatherum nelsonii] and bluebunch wheatgrass [Pseudoroegneria spicata]) across a range of fuel moistures.

Results
All three grass species had decreased flammability with increasing fuel moisture. Columbia needlegrass averaged 11% lower mass consumption than cheatgrass, and bluebunch wheatgrass had longer flaming duration and higher maximum temperatures than cheatgrass and Columbia needlegrass. The addition of cheatgrass to each perennial grass increased combined mass consumption, flaming duration, and flame heights. For these three attributes, the impact differed by the amount of cheatgrass in the mixture. Maximum and mean temperatures during perennial grass combustion were similar with and without cheatgrass addition. Some attributes of Columbia needlegrass flammability when burned with cheatgrass were higher than expected based on the flammability of each species, suggesting that Columbia needlegrass may be susceptible to pre-heating from combustion of cheatgrass. Conversely, the flammability of bluebunch wheatgrass and cheatgrass together had both positive and negative interactive effects, suggesting the impact on joint flammability from cheatgrass differs by perennial grass species.

Conclusions
This study provides experimental evidence supporting previous qualitative observations of cheatgrass flammability. Cheatgrass increased perennial grass sustainability and consumption, suggesting that cheatgrass poses a significant fire threat to native grasses regardless of moisture content. The study provides species-specific insights into flammability, which could be used to inform efforts to prevent or mitigate cheatgrass-induced wildfires.
The functional evolution of Korea ginseng: black ginseng
Authors: Lee Myoungsook 
Published: November 2024

AbstractKorea ginseng (Panax ginseng C.A. Meyer) has been used in traditional medicinal foods for more than 2000 years. Because of the growing interest in personalized healthcare to prevent diseases, white ginseng (WG) is technically evolving into highly qualified products with reinforced specific functionalities of ginsenosides, such as red ginseng (RG) or black ginseng (BG). Using the PubMed research engine, we found 225 BG-related published papers from 1995 to 2024. The most common papers (n = 46) were related to processing technologies, such as ginsenoside transformation, manufacturing processes and product evaluation. Reviewing papers on the functionality of BG with in vitro and in vivo models, they usually cover the basic mechanism of functionality, such as antioxidant (n = 38), antitumor (n = 28), menopausal (n = 26), anti-inflammatory (n = 24) and immune (n = 20) mechanisms. There are insufficient studies that are directly related to diseases such as obesity, diabetes mellitus, hypertension and liver disease; moreover, a significant randomized controlled trials study of BG was not found. In this study, we reviewed the research trends on the technologies and functionalities of BG in Korea and forecasted the potential of BG as a new material for healthcare in the industry.
Role of sgk1 in cancer: a bibliometric analysis from 2013 to 2023—review article
Authors: Abo- Elenien Wesam Ibrahim , Badawy Samira G. , Abouelenin Osama , Hussein Farhan Khaleel , Kumari Sakshi 
Published: November 2024

AbstractSerum and glucocorticoid-regulated kinase 1 (SGK1) plays a multifaceted role in cancer progression and treatment resistance. Its importance stems from its role in several cellular functions essential to cancer development, including metabolism, apoptosis, cell survival, and proliferation. In cancer, SGK1 expression is often dysregulated, leading to its overactivation or overexpression in many malignancies. This dysregulation can promote tumor growth, invasion, and metastasis through several mechanisms. We perform a bibliometric analysis to explore the role of SGK1 in cancer in the last ten years. All publications related to this topic were retrieved from the Scopus database. Microsoft Office Excel 2021 and VOSviewer (version 1.6.20) were used for the bibliometric analysis. The filtered search identified 5322 articles published between 2013 and 2023. Regarding research and scientific collaboration in this field, China is in the lead, followed by the USA, Germany, the UK, Japan, and Italy. In summary, SGK1 plays a critical role in cancer by regulating cell survival, proliferation, metabolism, and stemness. Its dysregulation promotes tumor growth, metastasis, and resistance to therapy. Targeting SGK1 is promising for enhancing cancer treatment efficacy, but further research is needed to fully understand its mechanisms and develop effective therapeutic strategies. Our study offers significant contributions to the field of cancer research by providing a comprehensive understanding of SGK1's role in cancer biology and highlighting its potential as a therapeutic target. These insights can inform future studies and guide the development of novel cancer prevention, diagnosis, and treatment approaches.
Investigations on stability improvement by implementing SSSC optimally with X/R ratio
Authors: Nema Shruti , George Sincy 
Published: November 2024

AbstractSeries compensation is widely used to enhance the transmittable power through an existing transmission line. This is achieved by controlling series reactance of the transmission line by injecting a voltage in series with transmission line and is perpendicular to the line current. However, X/R ratio of the transmission line gets deteriorated with series compensation. X/R ratio plays an important role in maintaining stability and damping oscillation in power system. In the proposed scheme, X/R ratio of the line has been included as one of the constraints along with midpoint voltage and power balance equation, with the objective of minimizing power system losses while enhancing the power. It is found that by maintaining X/R ratio of the transmission line same as the original designed value, stability of the system can be improved while making an attempt to enhance the transmittable power capacity. Steady state and transient stability, voltage stability and damping oscillations are analyzed with proposed optimal control of statis synchronous series compensator (SSSC), and results are produced. Validation of proposed optimal control of SSSC is done by using MATLAB platform for enhancing transmittable power of a 400 kV, 400 km transmission line and results are presented here. The paper investigates the effectiveness of the proposed optimization technique on improvement of power system stability and oscillations damping as well.
Improving lambs’ temperament, performance, and fecundity under three different housing conditions via oxidative stress reduction, metabolic and growth genes regulation by turmeric supplementation
Authors: Khalil Fatma , shehata Nashwa , Ibrahim Marwa A. , Nady Safaa , Emeash H. H. , Abdelghany Asmaa K. 
Published: November 2024

Abstract
Background
Improvement of performance, temperament, and fecundity of lambs using medicinal plants is considered a recent approach in the sheep industry. Climate change, especially in non-controlled houses, affects animals' production. Thus, the current study aimed to determine how turmeric (TU) affected the temperament, performance, and lamb fecundity under three different housing conditions. Thirty-six Ossimi male lambs (about 6 months of age) were randomly divided into three groups under three different housing conditions (n = 12; semi-shaded building, fully shaded building, and semi-shaded building with trees); in each house lambs were subdivided into non-treated (n = 6) and treated (n = 6; 1 g TU \lamb\day) groups for 3 months. Performance and temperament scoring have been recorded. Various biochemical parameters (total protein, glucose and oxidative damage), gene expression of metabolic function and growth indicators (insulin growth factor: IGF-1, leptin: LEP, and myostatin: MSTN), and testosterone level were measured.

Results
The obtained data showed that TU improved the lambs' temperament in SSB-T and FSB-T lambs; lambs' weight gain enhanced in treated lambs in all houses in the 3rd month. The results of biochemical parameters showed fluctuations throughout the experiment; total protein was elevated in FSB-NT lambs in the 3rd month, while glucose was elevated in SSB + T-NT in the 1st and 2nd month and then decreased in the 3rd month. The MDA elevated in FSB-T and SSB + T-T in the 2nd and 3rd month. TU supplementation elevated the reduced testosterone level in the SSB-T lambs and resulted in significant upregulation of IGF1, and LEP; however, MSTN downregulated in the treated lambs among all housing systems.

Conclusion
Data suggested TU improved lambs' growth and tempered in all houses and ameliorated the recorded reduction in testosterone levels in the SSB house which is commonly used in sheep and upregulated the IGF1 and LEP gene levels. Hence, the use of TU as a feed additive improved lambs' growth.
Neuromuscular diseases: genomics-driven advances
Authors: Cho Anna 
Published: November 2024

AbstractNeuromuscular diseases (NMDs) are a group of rare disorders characterized by significant genetic and clinical complexity. Advances in genomics have revolutionized both the diagnosis and treatment of NMDs. While fewer than 30 NMDs had known genetic causes before the 1990s, more than 600 have now been identified, largely due to the adoption of next-generation sequencing (NGS) technologies such as whole-exome sequencing (WES) and whole-genome sequencing (WGS). These technologies have enabled more precise and earlier diagnoses, although the genetic complexity of many NMDs continues to pose challenges. Gene therapy has been a transformative breakthrough in the treatment of NMDs. In spinal muscular atrophy (SMA), therapies like nusinersen, onasemnogene abeparvovec, and risdiplam have dramatically improved patient outcomes. Similarly, Duchenne muscular dystrophy (DMD) has seen significant progress, most notably with the FDA approval of delandistrogene moxeparvovec, the first micro-dystrophin gene therapy. Despite these advancements, challenges remain, including the rarity of many NMDs, genetic heterogeneity, and the high costs associated with genomic technologies and therapies. Continued progress in gene therapy, RNA-based therapeutics, and personalized medicine holds promise for further breakthroughs in the management of these debilitating diseases.