bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustainable and recyclable super engineering thermoplastic from biorenewable monomer
Authors: Park Seul-A , Jeon Hyeonyeol , Kim Hyungjun , Shin Sung-Ho , Choy Seunghwan , Hwang Dong Soo , Koo Jun Mo , Jegal Jonggeon , Hwang Sung Yeon , Park Jeyoung , Oh Dongyeop X. 
Published: June 2019

AbstractEnvironmental and health concerns force the search for sustainable super engineering plastics (SEPs) that utilise bio-derived cyclic monomers, e.g. isosorbide instead of restricted petrochemicals. However, previously reported bio-derived thermosets or thermoplastics rarely offer thermal/mechanical properties, scalability, or recycling that match those of petrochemical SEPs. Here we use a phase transfer catalyst to synthesise an isosorbide-based polymer with a high molecular weight >100 kg mol−1, which is reproducible at a 1-kg-scale production. It is transparent and solvent/melt-processible for recycling, with a glass transition temperature of 212 °C, a tensile strength of 78 MPa, and a thermal expansion coefficient of 23.8 ppm K−1. Such a performance combination has not been reported before for bio-based thermoplastics, petrochemical SEPs, or thermosets. Interestingly, quantum chemical simulations show the alicyclic bicyclic ring structure of isosorbide imposes stronger geometric restraint to polymer chain than the aromatic group of bisphenol-A.
Hypoxia-enhanced Blood-Brain Barrier Chip recapitulates human barrier function and shuttling of drugs and antibodies
Authors: Park Tae-Eun , Mustafaoglu Nur , Herland Anna , Hasselkus Ryan , Mannix Robert , FitzGerald Edward A. , Prantil-Baun Rachelle , Watters Alexander , Henry Olivier , Benz Maximilian , Sanchez Henry , McCrea Heather J. , Goumnerova Liliana Christova , Song Hannah W. , Palecek Sean P. , Shusta Eric , Ingber Donald E. 
Published: June 2019

AbstractThe high selectivity of the human blood-brain barrier (BBB) restricts delivery of many pharmaceuticals and therapeutic antibodies to the central nervous system. Here, we describe an in vitro microfluidic organ-on-a-chip BBB model lined by induced pluripotent stem cell-derived human brain microvascular endothelium interfaced with primary human brain astrocytes and pericytes that recapitulates the high level of barrier function of the in vivo human BBB for at least one week in culture. The endothelium expresses high levels of tight junction proteins and functional efflux pumps, and it displays selective transcytosis of peptides and antibodies previously observed in vivo. Increased barrier functionality was accomplished using a developmentally-inspired induction protocol that includes a period of differentiation under hypoxic conditions. This enhanced BBB Chip may therefore represent a new in vitro tool for development and validation of delivery systems that transport drugs and therapeutic antibodies across the human BBB.
Ab initio nonrigid X-ray nanotomography
Authors: Odstrcil Michal , Holler Mirko , Raabe Jörg , Sepe Alessandro , Sheng Xiaoyuan , Vignolini Silvia , Schroer Christian G. , Guizar-Sicairos Manuel 
Published: June 2019

AbstractReaching the full potential of X-ray nanotomography, in particular for biological samples, is limited by many factors, of which one of the most serious is radiation damage. Although sample deformation caused by radiation damage can be partly mitigated by cryogenic protection, it is still present in these conditions and, as we exemplify here using a specimen extracted from scales of theCyphochilusbeetle, it will pose a limit to the achievable imaging resolution. We demonstrate a generalized tomographic model, which optimally follows the sample morphological changes and attempts to recover the original sample structure close to the ideal, damage-free reconstruction. Whereas our demonstration was performed using ptychographic X-ray tomography, the method can be adopted for any tomographic imaging modality. Our application demonstrates improved reconstruction quality of radiation-sensitive samples, which will be of increasing relevance with the higher brightness of 4th generation synchrotron sources.
Uniform shape monodisperse single chain nanocrystals by living aqueous catalytic polymerization
Authors: Schnitte Manuel , Staiger Anne , Casper Larissa A. , Mecking Stefan 
Published: June 2019

AbstractThe preparation of polymer nanoparticles with a uniform size and shape, beyond spheres, is an unresolved problem. Here we report a living aqueous catalytic polymerization, resulting in particles grown by a single active site and composed of a single ultra high molecular weight polyethylene (UHMWPE) chain. The control on a molecular level (Mw/Mn = 1.1–1.2) and at the same time on a particle level (PDI < 0.05) together with the immediate deposition of the growing chain on the growing nanocrystal results in a distinct evolution of the particle morphology over time. These uniform nanocrystals are obtained as concentrated aqueous dispersions of > 10 wt-% (N ≈ 1019 particles L−1) polymer content. Key to this robust procedure to single chain nanoparticles are long-lived water-stable Ni(II) catalysts that do not undergo any chain transfer. These findings are a relevant step towards polymer materials based on nanoparticle assembly.
Secure quantum remote state preparation of squeezed microwave states
Authors: Pogorzalek S. , Fedorov K. G. , Xu M. , Parra-Rodriguez A. , Sanz M. , Fischer M. , Xie E. , Inomata K. , Nakamura Y. , Solano E. , Marx A. , Deppe F. , Gross R. 
Published: June 2019

AbstractQuantum communication protocols based on nonclassical correlations can be more efficient than known classical methods and offer intrinsic security over direct state transfer. In particular, remote state preparation aims at the creation of a desired and known quantum state at a remote location using classical communication and quantum entanglement. We present an experimental realization of deterministic continuous-variable remote state preparation in the microwave regime over a distance of 35 cm. By employing propagating two-mode squeezed microwave states and feedforward, we achieve the remote preparation of squeezed states with up to 1.6 dB of squeezing below the vacuum level. Finally, security of remote state preparation is investigated by using the concept of the one-time pad and measuring the von Neumann entropies. We find nearly identical values for the entropy of the remotely prepared state and the respective conditional entropy given the classically communicated information and, thus, demonstrate close-to-perfect security.
Atomic-scale mapping of hydrophobic layers on graphene and few-layer MoS2 and WSe2 in water
Authors: Uhlig Manuel R. , Martin-Jimenez Daniel , Garcia Ricardo 
Published: June 2019

AbstractThe structure and the role of the interfacial water in mediating the interactions of extended hydrophobic surfaces are not well understood. Two-dimensional materials provide a variety of large and atomically flat hydrophobic surfaces to facilitate our understanding of hydrophobic interactions. The angstrom resolution capabilities of three-dimensional AFM are exploited to image the interfacial water organization on graphene, few-layer MoS2 and few-layer WSe2. Those interfaces are characterized by the existence of a 2 nm thick region above the solid surface where the liquid density oscillates. The distances between adjacent layers for graphene, few-layer MoS2 and WSe2 are ~0.50 nm. This value is larger than the one predicted and measured for water density oscillations (~0.30 nm). The experiments indicate that on extended hydrophobic surfaces water molecules are expelled from the vicinity of the surface and replaced by several molecular-size hydrophobic layers.
Transcriptome profiles of three Muscat table grape cultivars to dissect the mechanism of terpene biosynthesis
Authors: Sun Lei , Zhu Baoqing , Zhang Xuanyin , Zhang Guojun , Yan Ailing , Wang Huiling , Wang Xiaoyue , Xu Haiying 
Published: June 2019

AbstractVitis viniferais widely grown worldwide for making wine and for use as table grapes. Of the existing cultivars, some have a floral and fruity flavour, referred to as a Muscat flavour. It is well-documented that this flavour originates from a series of terpene compounds, but the mechanism of terpene content differences among the Muscat-type cultivars remains unclear. Transcript and terpene metabolite profiles were integrated to elucidate the molecular mechanism of this phenomenon. In this research, three genotypes with different aromatic strengths were investigated by RNA sequencing. A total of 27 fruit samples from three biological replicates were sequenced on Illumina HiSeq2000 at three stages, corresponding to the veraison; berries had intermediate Brix value and were harvest-ripe. After quality assessment and data clearance, a total of 254.18 Gb of data with more than 97% Q20 bases were obtained, approximately 9.41 Gb data were generated per sample. These results will provide a valuable dataset for the discovery of the mechanism of terpene biosynthesis.
The ion channels and transporters gene expression profile indicates a shift in excitability and metabolisms during malignant progression of Follicular Lymphoma
Authors: Magi Alberto , Masselli Marika , Sala Cesare , Guerriero Angela , Laise Pasquale , Puccini Benedetta , Rigacci Luigi , Breschi Carla , Crociani Olivia , Pillozzi Serena , Arcangeli Annarosa 
Published: June 2019

AbstractThe definition of the gene expression profile of genes encoding Ion Channels and Transporters (ICT-GEP) represents a novel and attracting aspect in cancer. We determined the ICT-GEP of Follicular Lymphoma (FL), and compared it with that of the more aggressive Diffuse Large B Cell Lymphoma (DLBCL). cDNA microarray data were collected both from patients enrolled for this study, and from public datasets. In FL the ICT-GEP indicated the overexpression of both the K+ channel encoding gene KCNN4, and SLC2A1, which encodes the Glut1 glucose transporter. SLC2A1 turned out to represent the hub of a functional network, connecting channels and transporters in FL. Relapsed FL patients were characterised by 38 differentially expressed ICT genes, among which ATP9A, SLC2A1 and KCNN4 were under-expressed, indicating a down-regulation of both excitability and glycolysis. A completely different profile of K+ channel encoding genes emerged in DLBCL accompanied by the over-expression of the fatty acid transporter-encoding gene SLC27A1 as well as of the metabolism regulator NCoR1. This indicates a change in excitability and a shift towards an oxidative metabolism in DLBCL. Overall, the ICT-GEP may contribute to identifying novel lymphoma biomarkers related to excitability and metabolic pathways, with particular relevance for drug resistant, relapsed FL.
Antigenicity and immune correlate assessment of seven Plasmodium falciparum antigens in a longitudinal infant cohort from northern Ghana
Authors: Kusi Kwadwo Asamoah , Aguiar Joao , Kumordjie Selassie , Aggor Felix , Bolton Jessica , Renner Andrea , Kyei-Baafour Eric , Puplampu Naiki , Belmonte Maria , Dodoo Daniel , Gyan Ben Adu , Ofori Michael Fokuo , Oduro Abraham Rex , Atuguba Frank , Koram Kwadwo Ansah , Adams Nehkonti , Letizia Andrew , Villasante Eileen , Sedegah Martha 
Published: June 2019

AbstractThe current global malaria control and elimination agenda requires development of additional effective disease intervention tools. Discovery and characterization of relevant parasite antigens is important for the development of new diagnostics and transmission monitoring tools and for subunit vaccine development. This study assessed the natural antibody response profile of seven novel Plasmodium falciparum pre-erythrocytic antigens and their potential association with protection against clinical malaria. Antigen-specific antibody levels in plasma collected at six time points from a longitudinal cohort of one-to-five year old children resident in a seasonal malaria transmission area of northern Ghana were assessed by ELISA. Antibody levels were compared between parasite-positive and parasite-negative individuals and the association of antibody levels with malaria risk assessed using a regression model. Plasma antibody levels against five of the seven antigens were significantly higher in parasite-positive children compared to parasite-negative children, especially during low transmission periods. None of the antigen-specific antibodies showed an association with protection against clinical malaria. The study identified five of the seven antigens as markers of exposure to malaria, and these will have relevance for the development of disease diagnostic and monitoring tools. The vaccine potential of these antigens requires further assessment.
Patient-specific Computational Hemodynamic Analysis for Interrupted Aortic Arch in an Adult: Implications for Aortic Dissection Initiation
Authors: Peng Liqing , Qiu Yue , Yang Zhigang , Yuan Ding , Dai Chenzhong , Li Da , Jiang Yi , Zheng Tinghui 
Published: June 2019

AbstractThe guideline for the treatment of interrupted aortic arch (IAA) in adults has not been established although most centers tend to propose surgery. There is no clear evidence for the preferred selection of surgical repair versus conservatively medical treatment for the uncertain effects of both treatments. However, reports of sporadic aortic dissection (AD) of descending aorta (DAo) in IAA in adults before surgery drew our attention. It is quite perplexing because there seems to be no risk factors for the development of AD at DAo such as long-term uncontrolled hypertension, atherosclerosis, aortic aneurysm or genetic disorder. In this paper, we carried out the numerical investigation on the hemodynamics in a patient-specific IAA model, which was reconstructed from computed tomography images. Hemodynamic parameters including the flow pattern, pressure distribution, and wall shear stress (WSS) indicators were obtained. The simulation revealed that the jet flows from the collateral arteries (CAs) induced risk hemodynamic forces on the lumen wall including high time-averaged wall shear stress (TAWSS), high pressure and rapid change of WSS direction throughout the cardiac cycle. Moreover, it is found that only a jet flow which circumferentially washes out the aortic wall might cause tears on the wall. It is concluded that the specific geometrical features of the extensive major CAs might result in the risky hemodynamics leading to the initiation and development of AD in this particular IAA patient. CFD analysis in IAA can provide a clinical reference, and the results should be further studied in depth in the future.
Genetic Diversity and Demographic History of Globe Skimmers (Odonata: Libellulidae) in China Based on Microsatellite and Mitochondrial DNA Markers
Authors: Cao Ling-zhen , Wu Kong-ming 
Published: June 2019

AbstractTo analyze genetic characters of migratory dragonflies, we used 10 microsatellite markers and a partial sequence of the mitochondrial gene Cytb to investigate genetic diversity and demographic history among 19 populations of P. flavescens in eastern the monsoon region of China. In a Bayesian clustering analysis of the microsatellite data, three distinct clades were present, and each population consisted of a mixture of individuals from the three clusters. An AMOVA of the data from both the microsatellite loci and Cytb revealed that genetic variation was mainly within each population. For the 543 individuals from the 19 regions, 77 unique haplotypes were obtained by DnaSP 4.0, and a median-joining network showed no obvious geographical pattern and displayed high gene flow and minimal population genetic structure among the 19 populations. According to a Mantel test, there was no significant association between genetic distance and geographic distribution and no isolation by distance. Mismatch distribution and neutrality tests showed no demographic expansion for the 19 populations. Microsatellite and mitochondrial DNA data suggested there was high gene flow and low differentiation among the populations. These results will help provide valuable information to study the migratory route of insects, especially important agricultural pests.
A 30 Gb/s PAM4 underwater wireless laser transmission system with optical beam reducer/expander
Authors: Tsai Wen-Shing , Lu Hai-Han , Wu Hsiao-Wen , Su Chung-Wei , Huang Yong-Cheng 
Published: June 2019

AbstractWe have, so far as we know, proposed and demonstrated the first 30 Gb/s four-level pulse amplitude modulation (PAM4) underwater wireless laser transmission (UWLT) system with an optical beam reducer/expander over 12.5-m piped underwater channel/2.5-m high-turbidity harbour underwater channel. In piped underwater links, the performances of PAM4 UWLT systems get better with beam reduction given a small amount of light absorbed by the piped water. In highly turbid harbour underwater links, the performances of PAM4 UWLT systems get better with beam expansion given a large amount of scattered light received by the optical receiver. The effect of high-turbidity harbour water that induces scattering angle (beam divergence) on beam diameter is analyzed and optimised to enhance the transmission performances. This proposed PAM4 UWLT system, which uses an optical beam reducer/expander, provides a practical choice for high transmission capacity and considerably develops clarity and high-turbidity scenarios. It presents promising features for affording a high-transmission-rate underwater optical wireless transmission and opening an access to accelerate wide applications of UWLT systems.
Vascular tortuosity of the internal carotid artery is related to the RNF213 c.14429G > A variant in moyamoya disease
Authors: An Sungjae , Kim Tackeun , Oh Chang Wan , Bang Jae Seung , Lee Si Un , Heo Jaehyuk 
Published: June 2019

AbstractRecent studies have implicated RNF213 mutations in the pathogenesis of moyamoya disease (MMD). However, the underlying mechanism of disease development is not fully elucidated. Nonetheless, a possible relationship between vascular morphology and hemodynamics related with MMD has been proposed. Here, we aimed to investigate the relationship between a variant of RNF213 and the morphology of the internal carotid artery (ICA). We enrolled bilateral MMD patients who had undergone genetic testing for RNF213. Patients were divided into mutant and wild-type groups. Six anatomy-specific three-dimensional coordinates were collected using magnetic-resonance angiography. From these, five vectors between two adjacent points and four angles between two adjacent vectors were calculated. The tortuosity was defined as the ratio between the actual and the linear length of the ICAs. Among 58 patients, 44 and 14 belonged to the mutant and wild-type groups, respectively. The tortuosity of ICAs was significantly lower in the mutant group (p = 0.010). The change in blood flow direction was more prominent in the wild-type group (p = 0.002). The tortuosity was significantly lower in MMD patients than normal controls (p < 0.001). Our results indicate that RNF213 could play a role in the lower tortuosity observed in patients with RNF213 mutation.
A six-months study on Black Soldier Fly (Hermetia illucens) based diets in zebrafish
Authors: Zarantoniello Matteo , Randazzo Basilio , Truzzi Cristina , Giorgini Elisabetta , Marcellucci Claudia , Vargas-Abúndez Jorge Arturo , Zimbelli Andrea , Annibaldi Anna , Parisi Giuliana , Tulli Francesca , Riolo Paola , Olivotto Ike 
Published: June 2019

AbstractIntensive fish farming relies on the use of feeds based on fish meal and oil as optimal ingredients; however, further development of the aquaculture sector needs new, nutritious and sustainable ingredients. According to the concept of circular economy, insects represent good candidates as aquafeed ingredients since they can be cultured through environmental-friendly, cost-effective farming processes, on by-products/wastes, and many studies have recently been published about their inclusion in fish feed. However, information about the physiological effects of insect-based diets over the whole life cycle of fish is presently missing. At this regard, the present study investigated, for the first time, the effects of Black Soldier Fly based diets (25 and 50% fish meal substitution) administration for a six months period in zebrafish (Danio rerio), from larvae to adults. A multidisciplinary approach, including biometric, biochemical, histological, spectroscopic and molecular analyses was applied. Aside a general reduction in fish growth and lipid steatosis, six-months feeding on Black Soldier Fly based diets did not show major negative effects on zebrafish. Gut histological analysis on intestine samples did not show signs of inflammation and both stress markers and immune response markers did not show significant differences among the experimental groups.
Enzymatic fluorometric assays for quantifying all major phospholipid classes in cells and intracellular organelles
Authors: Tsuji Tokuji , Morita Shin-ya , Ikeda Yoshito , Terada Tomohiro 
Published: June 2019

AbstractCell membrane phospholipids regulate various biological functions. We previously reported enzymatic fluorometric methods for quantifying phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin, phosphatidylglycerol and cardiolipin. In the present report, a new enzymatic fluorometric assay was developed for quantifying phosphatidylinositol. These simple, sensitive and high-throughput methods enabled us to quantify all major phospholipid classes in cultured cells and intracellular organelles. By conducting comprehensive quantitative analyses of major phospholipid classes, we demonstrated that the contents of phospholipid classes in HEK293 cells changed with cell density and that overexpression of phosphatidylinositol synthase or CDP-diacylglycerol synthase significantly affected the phospholipid compositions of microsomal and mitochondrial membranes. These enzymatic fluorometric assays for measuring all major phospholipid classes may be applicable to tissues, fluids, lipoproteins, extracellular vesicles and intracellular organelles of many organisms and will further our understanding of cellular, physiological and pathological processes.
Enlarged representation of peripersonal space in pregnancy
Authors: Cardini Flavia , Fatemi-Ghomi Natalie , Gajewska-Knapik Katarzyna , Gooch Victoria , Aspell Jane Elizabeth 
Published: June 2019

AbstractOur ability to maintain a coherent bodily self despite continuous changes within and outside our body relies on the highly flexible multisensory representation of the body, and of the space surrounding it: the peripersonal space (PPS). The aim of our study was to investigate whether during pregnancy - when extremely rapid changes in body size and shape occur - a likewise rapid plastic reorganization of the neural representation of the PPS occurs. We used an audio-tactile integration task to measure the PPS boundary at different stages of pregnancy. We found that in the second trimester of pregnancy and postpartum women did not show differences in their PPS size as compared to the control group (non-pregnant women). However, in the third trimester the PPS was larger than the controls’ PPS and the shift between representation of near and far space was more gradual. We therefore conclude that during pregnancy the brain adapts to the sudden bodily changes, by expanding the representation of the space around the body. This may represent a mechanism to protect the vulnerable abdomen from injury from surrounding objects.