bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and characterization of TP53 gene Allele Dropout in Li-Fraumeni syndrome and Oral cancer cohorts
Authors: Haque Mohammed Moquitul , Kowtal Pradnya , Sarin Rajiv 
Published: July 2018

AbstractAllele Drop out (ADO) arising from non-amplification of one allele may produce false negative result and impact clinical management. In cancer, germline and somatic genetic analysis is being increasingly used but the prevalence, nature and implications of ADO has not been studied in any cohort. In a cohort of 290 Li Fraumeni/Li Fraumeni Like Syndrome cases undergoing TP53 genetic testing, of the 69 pathogenic mutations identified so far, 5 were initially missed and 4 were misgenotyped as homozygous mutation due to germline ADO. Of the 9 germline ADOs, 8 were sequence dependent, arising from a polymorphism (rs12951053) in the primer annealing region of exon 7. Of 35 somatic TP53 variants identified by exome sequencing in 50 oral cancer tissues registered under International Cancer Genome Consortium (ICGC), as a result of ADO, 4 were not detectable and 6 were not called as variant on Sanger Sequencing due to low peak height. High prevalence of germline and somatic ADO in the most frequently mutated cancer gene TP53, highlights the need for systematic evaluation of ADO prevalence and causes in clinically important cancer genes. False negative result for high penetrance germline mutations or actionable somatic mutations in oncogenes could have major clinical implications.
A framework for large-scale metabolome drug profiling links coenzyme A metabolism to the toxicity of anti-cancer drug dichloroacetate
Authors: Dubuis Sébastien , Ortmayr Karin , Zampieri Mattia 
Published: July 2018

AbstractMetabolic profiling of cell line collections has become an invaluable tool to study disease etiology, drug modes of action and to select personalized treatments. However, large-scale in vitro dynamic metabolic profiling is limited by time-consuming sampling and complex measurement procedures. By adapting a mass spectrometry-based metabolomics workflow for high-throughput profiling of diverse adherent mammalian cells, we establish a framework for the rapid measurement and analysis of drug-induced dynamic changes in intracellular metabolites. This methodology is scalable to large compound libraries and is here applied to study the mechanism underlying the toxic effect of dichloroacetate in ovarian cancer cell lines. System-level analysis of the metabolic responses revealed a key and unexpected role of CoA biosynthesis in dichloroacetate toxicity and the more general importance of CoA homeostasis across diverse human cell lines. The herein-proposed strategy for high-content drug metabolic profiling is complementary to other molecular profiling techniques, opening new scientific and drug-discovery opportunities.
First core properties: from low- to high-mass star formation
Authors: Bhandare Asmita , Kuiper Rolf , Henning Thomas , Fendt Christian , Marleau Gabriel-Dominique , Kölligan Anders 
Published: July 2018

Aims. In this study, the main goal is to understand the molecular cloud core collapse through the stages of first and second hydrostatic core formation. We investigate the properties of Larsons first and second cores following the evolution of the molecular cloud core until the formation of Larson’s cores. We expand these collapse studies for the first time to span a wide range of initial cloud masses from 0.5 to 100 M⊙.
Methods. Understanding the complexity of the numerous physical processes involved in the very early stages of star formation requires detailed thermodynamical modelling in terms of radiation transport and phase transitions. For this we used a realistic gas equation of state via a density- and temperature-dependent adiabatic index and mean molecular weight to model the phase transitions. We used a grey treatment of radiative transfer coupled with hydrodynamics to simulate Larsons collapse in spherical symmetry.
Results. We reveal a dependence of a variety of first core properties on the initial cloud mass. The first core radius and mass increase from the low-mass to intermediate-mass regime and decrease from the intermediate-mass to high-mass regime. The lifetime of first cores strongly decreases towards the intermediate- and high-mass regimes.
Conclusions. Our studies show the presence of a transition region in the intermediate-mass regime. Low-mass protostars tend to evolve through two distinct stages of formation that are related to the first and second hydrostatic cores. In contrast, in the high-mass star formation regime, collapsing cloud cores rapidly evolve through the first collapse phase and essentially immediately form Larson’s second cores.
Antithrombotische Therapie bei peripherer arterieller Verschlusskrankheit
Authors: Espinola-Klein Christine 
Published: July 2018

Was ist neu?
Antithrombotische Therapie bei stabiler PAVK Die aktuellen deutschen und europäischen Leitlinien empfehlen bei Patienten mit einer peripheren arteriellen Verschlusskrankheit (PAVK) die Monotherapie mit einem Thrombozytenaggregationshemmer (ASS 100 mg oder Clopidogrel 75 mg).In der COMPASS (Cardiovascular OutcoMes for People using Anticoagulation StrategieS) -Studie wurde Patienten mit PAVK 2 × 2,5 mg Rivaroxaban zusätzlich zu ASS 100 mg gegeben. Dies führte zur signifikanten Reduktion kardiovaskulärer Ereignisse (MACE = Major Adverse Cardiovascular Events) wie kardiovaskulärer Tod, Myokardinfarkt und Schlaganfall. Ebenfalls signifikant reduziert wurden periphere Ereignisse (MALE = Major Adverse Limb Events) wie ischämiebedingte Major-Amputation und eine akute schwere Ischämie.Liegt bei Patienten mit einer PAVK die Indikation zur oralen Antikoagulation vor (z. B. bei Vorhofflimmern), empfehlen die aktuellen deutschen und europäischen Leitlinien die Monotherapie mit oralen Antikoagulantien ohne zusätzliche Thrombozytenaggregationshemmung.
Antithrombotische Therapie nach peripherer Revaskularisation Nach peripherer Intervention wird in Analogie zur Koronarintervention meist passager eine duale Plättchenhemmung durchgeführt. Nach peripherer Bypass-Anlage wird in der Regel die Monotherapie mit einem Thrombozytenaggregationshemmer empfohlen. In Einzelfällen kann bei komplexem Venenbypass eine orale Antikoagulation und bei Kunststoffbypass eine duale Plättchenhemmung eingesetzt werden.
The Influence of Smoking and of Oral and Transdermal Nicotine on Blood Pressure, and Haematology and Coagulation Indices
Authors: Blann Andrew D , Steele Christopher , McCollum Charles N 
Published: July 2018

SummaryNicotine is helpful in stopping smoking but its influence on cardiovascular risk factors is incomplete. Our aim was to determine its effect on blood pressure, routine haematology indices, and coagulation indices relevant to thrombosis. Eighteen subjects were seen whilst smoking (cotinine levels 1119 ± 414 ng/ml), again after stopping smoking but while using nicotine chewing gum and/or skin patches (392 ± 198 ng/ml), and again when not using nicotine (cotinine undetectable). There were no significant changes in blood pressures, platelet count, mean platelet volume, viscosity or anti-thrombin III. However, white blood cell count (p = 0.003), lymphocyte count (p = 0.016), red blood cell count (p = 0.02), haemoglobin (p <0.001), fibrinogen (p <0.001) and von Willebrand factor (p = 0.001) all fell between the first and second samples (when still using nicotine) but not between the second and third samples (when off nicotine). Oral and/or transdermal nicotine does not influence blood pressure or the haematology and coagulation indices we have measured.
Clinical Impact of Bleeding in Cancer-Associated Venous Thromboembolism: Results from the Hokusai VTE Cancer Study
Authors: Kraaijpoel Noémie , Di Nisio Marcello , Mulder Frits , van Es Nick , Beyer-Westendorf Jan , Carrier Marc , Garcia David , Grosso Michael , Kakkar Ajay , Mercuri Michele , Middeldorp Saskia , Hernandez Cristhiam , Santamaria Amparo , Schwocho Lee , Segers Annelise , Verhamme Peter , Wang Tzu-Fei , Weitz Jeffrey , Zhang George , Zwicker Jeffrey , Büller Harry , Raskob Gary 
Published: July 2018

In the Hokusai VTE Cancer study, edoxaban was non-inferior to dalteparin for the composite outcome of recurrent venous thromboembolism (VTE) and major bleeding in 1,050 patients with cancer-associated VTE. The absolute rate of recurrent VTE was 3.4% lower with edoxaban, whereas the absolute rate of major bleeding was 2.9% higher. The present analysis focuses on the sites, clinical presentation, course and outcome of bleeding events, and the associated tumour types. Major bleeds and their severity (categories 1–4) were blindly adjudicated by a committee using a priori defined criteria, and data were analysed in the safety population. Major bleeding occurred in 32 of 522 patients given edoxaban (median treatment duration, 211 days) and in 16 of 524 patients treated with dalteparin (median treatment duration, 184 days); no patients had more than one major bleed. There were no fatal bleeds with edoxaban, and two with dalteparin. Severe bleeding at presentation (category 3 or 4) occurred in 10 (1.9%) and 11 (2.1%) patients in the edoxaban and dalteparin groups, respectively. The excess of major bleeding with edoxaban was confined to patients with gastrointestinal cancer. However, severe major bleeding at presentation (category 3 or 4) in this sub-group occurred in 5 of 165 (3.0%) and in 3 of 140 (2.1%) patients given edoxaban or dalteparin, respectively.In conclusion, this analysis suggests that while oral edoxaban is an appropriate alternative to subcutaneous dalteparin for treatment of cancer-associated VTE, the use of edoxaban in patients with gastrointestinal cancer requires careful benefit–risk weighting.
Antimicrobial Activity of Different Solutions on Denture Base Materials
Authors: Tuncdemir Ali , Uğur Ayşe , Kahraman Kübra , Özdemir Bilgen 
Published: July 2018

AbstractThere are a few chemical solutions used for prosthetic cleaning and they have different antimicrobial effects on denture materials. The aim of this study is to evaluate the efficacy of various chemical solutions used in prosthetic cleaning. For this purpose, 40 specimens of polymethylmethacrylate (PMMA-Meliodent, HeraeusKulzer, Hanau, Germany), which is the most commonly used material in conventional prostheses, and 40 specimens of Deflex (Nuxen SRL, Ayacucho 1053 3-A, Cap. Fed. Buenos Aires, Argentina), which is polyamide-containing semi-flexible acrylic, were molded using a template at the laboratory of Dentistry Faculty. Leveling began with abrasive burs and Stones followed by the sanding with medium and thin discs. Surface preparation was done using wet pumice and felt followed by polishing with gypsum-alcohol and rotary soft brush. The evaluated three solutions are Corega cleaning tablets containing alkaline peroxide, 0.2% chlorhexidine gluconate (CG) and 1% sodium hypochlorite (NaOCl). Distilled water was used as the control group. When both groups of polymers were evaluated in respect to the Candida growth after they were kept in liquid containing NaOCl, Corega, and CG, it was observed that only the acrylics that were kept in Corega revealed Candida growth. It was concluded that Corega could not sufficiently inhibit the growth of Candida albicans which is the one of the most common microorganisms in the oral flora of pediatric and elderly patients. NaOCl and CG can be used safely and effectively for denture cleaning.
Mechanical and tribological behaviours of aluminium matrix composites reinforced by graphene nanoplatelets
Authors: Şenel Mahmut Can , Gürbüz Mevlüt , Koç Erdem 
Published: July 2018

In the present study, the effects of graphene content (0.1, 0.3, 0.5 wt-%) on the mechanical and tribological properties of aluminium matrix composites were reported. The experimental results reveal that the best apparent density (2.58 ± 0.02 g cm−3), highest Vickers hardness (57 ± 2.5 HV), lowest mass loss (1.6 and 9.7 mg for 10 N and 40 N), and lowest wear rate (12 × 10−5 and 18 × 10−5 mm3/Nm for 10 N and 40 N) were obtained at aluminium–0.1% graphene composite when compared with pure aluminium. The ultimate compressive strength of composites increases from 106 ± 4 to 138 ± 4 MPa with increasing graphene nanoplatelet contents. All results showed that graphene has been a very effective reinforcement and solid-lubricant material for Al matrix composites.
Montmorency tart cherry (Prunus cerasus L.) supplementation accelerates recovery from exercise‐induced muscle damage in females
Authors: Brown Meghan A. , Stevenson Emma J. , Howatson Glyn 
Published: July 2018

AbstractTart Montmorency cherry concentrate (MC) has been reported to attenuate the symptoms of exercise‐induced muscle damage (EIMD) and to accelerate exercise recovery, which has been attributed to its high anti‐inflammatory and antioxidant properties. Although these data are promising, there are no data regarding exclusively female populations. Therefore, the aim of this investigation was to examine the efficacy of MC on recovery following EIMD in females. In a randomised, double‐blind, placebo‐controlled study, twenty physically active females (mean ± SD age 19 ± 1 y; stature 167 ± 6 cm; body mass 61.4 ± 5.7 kg) consumed MC or a placebo (PL) for eight days (30 mL twice per day). Following four days of supplementation, participants completed a repeated‐sprint protocol and measures of muscle soreness (DOMS), pain pressure threshold (PPT), limb girth, flexibility, muscle function, and systemic indices of muscle damage and inflammation were collected pre, immediately post (0 h) and 24, 48 and 72 h post‐exercise. Time effects were observed for all dependent variables (p < 0.05) except limb girth and high sensitivity C‐reactive protein. Recovery of countermovement jump height was improved in the MC group compared to PL (p = 0.016). There was also a trend for lower DOMS (p = 0.070) and for higher PPT at the rectus femoris (p = 0.071) in the MC group. The data demonstrate that MC supplementation may be a practical nutritional intervention to help attenuate the symptoms of muscle damage and improve recovery on subsequent days in females.
Design, construction, and stability test of aerial wireless coastal buoy
Authors: Wahidin L O , Jaya I , Atmadipoera A S 
Published: July 2018

Abstract
Due to its highly dynamic nature, coastal waters are necessary to be continuously monitor. Aerial wireless coastal buoy (AWCB) is one of the ocean instrument commonly deployed for observing oceanographic phenomena in coastal waters and around small islands. In this paper we describe the design, construction and the stability test result of new AWCB. The new AWCB is using water temperature sensors to collect data and transferring them through specific aerial wavelengths at real time and low cost in coastal areas. The AWCB is completed three steps. First, the buoy was designed using CAD software to determine its size, form and color. Next, the buoy was constructed using material from fiberglass. Last buoy was tested for its buoyancy and stability at the Acoustic and Instrument Laboratory, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University. At sea-trial was also carried out around Pramuka Island, and during the trial it shows that the buoy is stable condition within waves swaying and surface water current. It is floating well on seawater surface and stable for six days installing duration.
Community resilience and narratives of community empowerment in Scotland
Authors: Revell Philip , Dinnie Elizabeth 
Published: July 2018

Abstract
The concept of resilience is now routinely put forward by both policymakers and social activists as a way for communities to use and develop their resources and respond positively to change, including decarbonization to address climate change. The extent to which a community is able to utilize all its resources depends on the extent to which it feels empowered to take action and is a major determinant of its resilience. A narrative of community empowerment has recently emerged from Scottish Government, driven in part by the situation in Scotland, in which a skewed pattern of landownership and distant structure of ‘local’ democracy combine to disempower communities by disconnecting them from local resources and local representative democracy. Recent Scottish legislation appears to provide new opportunities for community groups to gain control of local assets, become more financially sustainable, undertake climate-related mitigation actions and overcome some of the current local democratic deficit. At the same time, an increasingly well-organized and networked community sector and some within the Scottish Government are actively exploring new ways to enable public participation and deliver public services. This paper analyses the current Scottish policy framework and aspirations for community empowerment and, through interviews with stakeholders, assesses the potential, this may provide for communities to become truly resilient and to actively engage with transformational change.
Stability and Change in Health Behavior Profiles of U.S. Adults
Authors: Burgard Sarah A , Lin Katherine Y P , Segal Brian D , Elliott Michael R , Seelye Sarah 
Published: July 2018

Abstract

Objectives
While understanding of complex within-person clustering of health behaviors into meaningful profiles of risk is growing, we still know little about whether and how U.S. adults transition from one profile to another as they age. This study assesses patterns of stability and change in profiles of tobacco and alcohol use and body mass index (BMI).

Method
A nationally representative cohort of U.S. adults 25 years and older was interviewed up to 5 times between 1986 and 2011. Latent transition analysis (LTA) models characterized the most common profiles, patterning of transitions across profiles over follow-up, and assessed whether some were associated with higher mortality risk.

Results
We identified 5 profiles: “health promoting” with normal BMI and moderate alcohol consumption; “overweight”; “current smokers”; “obese”; and “nondrinkers”. Profile membership was largely stable, with the most common transitions to death or weight gain. “Obese” was the most stable profile, while “smokers” were most likely to transition to another profile. Mortality was most frequent in the “obese” and “nondrinker” profiles.

Discussion
Stability was more common than transition, suggesting that adults sort into health behavior profiles relatively early. Women and men were differently distributed across profiles at baseline, but showed broad similarity in transitions.
Characterizing Workflow to Inform Clinical Decision Support Systems in Nursing Homes
Authors: Ozkaynak Mustafa , Reeder Blaine , Drake Cynthia , Ferrarone Peter , Trautner Barbara , Wald Heidi 
Published: July 2018

Abstract

Background and Objectives
Clinical decision support systems (CDSS) hold promise to influence clinician behavior at the point of care in nursing homes (NHs) and improving care delivery. However, the success of these interventions depends on their fit with workflow. The purpose of this study was to characterize workflow in NHs and identify implications of workflow for the design and implementation of CDSS in NHs.

Research Design and Methods
We conducted a descriptive study at 2 NHs in a metropolitan area of the Mountain West Region of the United States. We characterized clinical workflow in NHs, conducting 18 observation sessions and interviewing 15 staff members. A multilevel work model guided our data collection and framework method guided data analysis.

Results
The qualitative analysis revealed specific aspects of multilevel workflow in NHs: (a) individual, (b) work group/unit, (c) organization, and (d) industry levels. Data analysis also revealed several additional themes regarding workflow in NHs: centrality of ongoing relationships of staff members with the residents to care delivery in NHs, resident-centeredness of care, absence of memory aids, and impact of staff members’ preferences on work activities. We also identified workflow-related differences between the two settings.

Discussion and Implications
Results of this study provide a rich understanding of the characteristics of workflow in NHs at multiple levels. The design of CDSS in NHs should be informed by factors at multiple levels as well as the emergent processes and contextual factors. This understanding can allow for incorporating workflow considerations into CDSS design and implementation.
FOREST Unbiased Galactic Plane Imaging Survey with the Nobeyama 45 m telescope (FUGIN). IV. Galactic shock wave and molecular bow shock in the 4 kpc arm of the Galaxy
Authors: Sofue Yoshiaki , Kohno Mikito , Torii Kazufumi , Umemoto Tomofumi , Kuno Nario , Tachihara Kengo , Minamidani Tetsuhiro , Fujita Shinji , Matsuo Mitsuhiro , Nishimura Atsushi , Tsuda Yuya , Seta Masumichi 
Published: July 2018

Abstract
The FUGIN CO survey revealed the three-dimensional structure of a galactic shock wave in the tangential direction of the 4 kpc molecular arm. The shock front is located at G30.5+00.0 + 95 km s−1 on the upstream (lower longitude) side of the star-forming complex W 43 (G30.8−0.03), and comprises a molecular bow shock (MBS) concave to W 43, exhibiting an arc-shaped molecular ridge perpendicular to the galactic plane with width ∼0${^{\circ}_{.}}$1(10 pc) and vertical length ∼1° (100 pc). The MBS is coincident with the radio continuum bow of thermal origin, indicating association of ionized gas and similarity to a cometary bright-rimmed cloud. The upstream edge of the bow is sharp, with a growth width of ∼0.5 pc indicative of the shock front property. The velocity width is ∼10 km s−1, and the center velocity decreases by ∼15 km s−1 from the bottom to the top of the bow. The total mass of molecular gas in the MBS is estimated to be ∼1.2 × 106 M⊙, and ionized gas ∼2 × 104 M⊙. The vertical disk thickness has a step-like increase at the MBS by ∼2 times from lower to upper longitudes, which indicates hydraulic jump in the gaseous disk. We argue that the MBS was formed by the galactic shock compression of an accelerated flow in the spiral-arm potential encountering the W 43 molecular complex. A bow-shock theory can reproduce the bow morphology well. We argue that molecular bows are common in galactic shock waves, not only in the Galaxy but also in galaxies, where MBSs are associated with giant cometary H ii regions. We also analyzed the H i data in the same region to obtain a map of H i optical depth and molecular fraction. We found firm evidence of the H i to H2 transition in the galactic shock as revealed by a sharp molecular front at the MBS front.