bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nucleolin protects against doxorubicin‐induced cardiotoxicity via upregulating microRNA‐21
Authors: Sun Hui , Tong Zhongyi , Fang Yeqing , Jiang Bimei , Liang Pengfei , Tang Yuting , Li Yuanbin , Wu Yanyang , Xiao Xianzhong 
Published: July 2018

Nucleolin is a multifunctional protein and participates in many important biological processes. Our previous study found that nucleolin protects the heart against myocardial ischemia–reperfusion injury. In this study, we aimed to investigate the role of nucleolin in doxorubicin (DOX)‐induced cardiotoxicity. The expression pattern of nucleolin in hearts subjected to DOX injury was investigated, and we found that administration of DOX induced nucleolin expression significantly in vivo and in vitro. Gene transfection and RNA interference approaches were used in cardiomyocytes to investigate the function of nucleolin. Nucleolin overexpression protects cardiomyocytes against DOX‐induced injury. Nucleolin‐ablated cardiomyocytes become susceptible to the injury induced by DOX. The hearts of cardiac‐myocyte‐specific nucleolin transgenic mice are more resistant to DOX injury. Furthermore, nucleolin upregulates microRNA(miRNA)‐21 expression in vivo and in vitro, and the miRNA‐21 inhibitor negates the protective effect of nucleolin against injury induced by DOX. These results have demonstrated that nucleolin is involved in the regulation of DOX‐induced cardiac injury and dysfunction via the regulation of miRNA‐21 expression, and may be a novel therapeutic target for DOX‐induced cardiotoxicity.
MicroRNA miR-223 as regulator of innate immunity
Authors: Yuan Xiaoyi , Berg Nathaniel , Lee Jae Woong , Le Thanh-Thuy , Neudecker Viola , Jing Na , Eltzschig Holger 
Published: July 2018

Abstract
MicroRNAs were discovered more than 2 decades ago and have profound impact on diverse biological processes. Specific microRNAs have important roles in modulating the innate immune response and their dysregulation has been demonstrated to contribute to inflammatory diseases. MiR-223 in particular, is very highly expressed and tightly regulated in hematopoietic cells. It functions as key modulator for the differentiation and activation of myeloid cells. The central role of miR-223 in myeloid cells, especially neutrophil and macrophage differentiation and activation has been studied extensively. MiR-223 contributes to myeloid differentiation by enhancing granulopoiesis while inhibiting macrophage differentiation. Uncontrolled myeloid activation has detrimental consequences in inflammatory disease. MiR-223 serves as a negative feedback mechanism controlling excessive innate immune responses in the maintenance of myeloid cell homeostasis. This review summarizes several topics covering the function of miR-223 in myeloid differentiation, neutrophil and macrophage functions, as well as in inflammatory diseases including acute respiratory distress syndrome and inflammatory bowel disease. In addition, nonmyeloid functions of miR-223 are also discussed in this review. Therapeutic enhancement of miR-223 to dampen inflammatory targets is also highlighted as potential treatment to control excessive innate immune responses during mucosal inflammation.
MiR-223 is important in regulating innate immune responses and recent studies highlight the therapeutic potential of enhancing miR-223 as a treatment for inflammatory diseases.
The Embodied Experience of Microaggressions: Implications for Clinical Practice
Authors: Johnson Rae , Leighton Lucia , Caldwell Christine 
Published: July 2018

Ongoing scholarship in multicultural counseling practice has established the significance of microaggressions in the lived experience of clients and in the client–counselor dyad. Grounded in new research into the embodied experience of oppression, this article reviews key concepts in nonverbal communication and traumatology to illustrate how the body is implicated in the transmission of microaggressions and how counselors can become more attentive, informed, and responsive to how social power differences manifest in the body.Las investigaciones actuales sobre la práctica de la consejería multicultural han establecido la importancia de las microagresiones en la experiencia vital de los clientes y en la díada cliente–consejero. Fundamentado en nuevas investigaciones sobre la experiencia corporal de la opresión, este artículo revisa conceptos clave de la comunicación no verbal y la traumatología para ilustrar cómo el cuerpo está involucrado en la transmisión de microagresiones y cómo los consejeros pueden tornarse más atentos, informados y sensibles a cómo las diferencias de poder social se manifiestan en el cuerpo.
Counselor Education Students’ Perceptions of Wellness and Mental Health in African American Men: The Effects of Colorism
Authors: Hairston Tiffany R. , Laux John M. , O’Hara Caroline , Roseman Christopher P. , Gore Shanda 
Published: July 2018

Colorism is discrimination based on skin tone. Skin color influences clinicians’ diagnostic impressions. The degree to which colorism influences counseling students’ clinical decision making is unknown. This study examined colorism's effect on counselor education master's students’ (N = 154) clinical decisions. Analysis of covariance failed to produce statistically significant results when comparing students’ evaluations of a male African American's mental health and wellness, when controlling for presentation management, across 4 skin tones. Counselors should include colorism in diversity training.El colorismo es la discriminación basada en el tono de la piel. El tono de la piel influye en las impresiones diagnósticas de los profesionales clínicos. Se desconoce hasta qué punto el colorismo influye en las decisiones clínicas de los estudiantes de consejería. Este estudio examinó el efecto del colorismo en las decisiones clínicas de estudiantes de maestría en educación de consejeros (N = 154). El análisis de la covarianza no produjo resultados estadísticamente significativos al comparar las evaluaciones de los estudiantes sobre la salud mental y el bienestar de un cliente afroamericano varón, controlando la gestión de la presentación de 4 tonos de piel diferentes. Los consejeros deberían incluir el colorismo en la capacitación de diversidad.
Effect of microtexturing on powder boundary layer: An experimental study based on laser microscopy and profilometry measurements
Authors: Jiao Yunlong , Ji Jiawei , Liu Kun , Wang Wei 
Published: July 2018

AbstractPowder lubrication can effectively reduce friction under extreme conditions, but powder filling and storage are yet to be improved. In this study, the evolution of the powder boundary layer on microtextured surfaces is investigated. The effect of load and speed on the friction properties of the microtexutured surface is also revealed by using a multifunctional friction and wear tester. Interrupted laser microscopy and profilometry measurements of the friction surface show 4 evolution stages of the powder boundary layer development: localised formation, complete formation, local spalling, and large scuffing of the boundary layer. The steady state boundary layer is characterised with many tadpole‐shaped units and exhibits better lubricity under high load and speed, which effectively delay the damage and improve the life of frictional samples. The present work may provide some new insights to engineers and researchers in designing surface micro/nanotextures with better lubricity and wear resistance.
Copper‐Free Synthesis of Glycidyl Triazolyl Polymers
Authors: Ikeda Taichi 
Published: July 2018

AbstractFive types of glycidyl triazolyl polymers (GTPs) are synthesized without a copper catalyst by thermal azide‐alkyne cycloaddition reaction between glycidyl azide polymer and the electron‐deficient alkynes with the ester group. The samples are characterized by NMR, IR, size‐exclusion chromatography, differential scanning calorimetry, and thermogravimetric analysis. The terminal alkyne affords a 9:1 mixture of the 4‐ and 5‐substituted 1,2,3‐triazolyl derivatives of GTPs. The asymmetric internal alkyne with the methyl‐ and (triethylene glycol)‐esters gives an almost 1:1 mixture of the structural isomers of GTPs having the methyl‐ester substituent at either the 4‐ or 5‐positions of the 1,2,3‐triazole ring. The chemical modification by triethylene glycol improves thermal stability of the ester bond in GTP. The 4,5‐disubstituted triazolyl derivative of GTP has lower glass transition temperature than the mono‐substituted one. This achievement facilitates the work‐up process of GTP synthesis and contributes to expanding the application field of GTP, especially, to the systems not allowing the contamination of a copper catalyst in the biomedical field.
Newton‐Krylov method for computing the cyclic steady states of evolution problems in nonlinear mechanics
Authors: Khristenko Ustim , Le Tallec Patrick 
Published: July 2018

SummaryThis work is focused on the Newton‐Krylov technique for computing the steady cyclic states of evolution problems in nonlinear mechanics with space‐time periodicity conditions. This kind of problems can be faced, for instance, in the modeling of a rolling tire with a periodic tread pattern, where the cyclic state satisfies “rolling” periodicity condition, including shifts both in time and space. The Newton‐Krylov method is a combination of a Newton nonlinear solver with a Krylov linear solver, looking for the initial state, which provides the space‐time periodic solution. The convergence of the Krylov iterations is proved to hold in presence of an adequate preconditioner. After preconditioning, the Newton‐Krylov method can be also considered as an observer‐controller method, correcting the transient solution of the initial value problem after each period. Using information stored while computing the residual, the Krylov solver computation time becomes negligible with respect to the residual computation time. The method has been analyzed and tested on academic applications and compared with the standard evolution (fixed point) method. Finally, it has been implemented into the Michelin industrial code, applied to a full 3D rolling tire model.
Consensus molecular subtype classification of colorectal adenomas
Authors: Komor Malgorzata A , Bosch Linda JW , Bounova Gergana , Bolijn Anne S , Delis‐van Diemen Pien M , Rausch Christian , Hoogstrate Youri , Stubbs Andrew P , de Jong Mark , Jenster Guido , van Grieken Nicole CT , Carvalho Beatriz , Wessels Lodewyk FA , Jimenez Connie R , Fijneman Remond JA , Meijer Gerrit A ,   In collaboration with the NGS‐ProToCol Consortium:
Published: July 2018

AbstractConsensus molecular subtyping is an RNA expression‐based classification system for colorectal cancer (CRC). Genomic alterations accumulate during CRC pathogenesis, including the premalignant adenoma stage, leading to changes in RNA expression. Only a minority of adenomas progress to malignancies, a transition that is associated with specific DNA copy number aberrations or microsatellite instability (MSI). We aimed to investigate whether colorectal adenomas can already be stratified into consensus molecular subtype (CMS) classes, and whether specific CMS classes are related to the presence of specific DNA copy number aberrations associated with progression to malignancy. RNA sequencing was performed on 62 adenomas and 59 CRCs. MSI status was determined with polymerase chain reaction‐based methodology. DNA copy number was assessed by low‐coverage DNA sequencing (n = 30) or array‐comparative genomic hybridisation (n = 32). Adenomas were classified into CMS classes together with CRCs from the study cohort and from The Cancer Genome Atlas (n = 556), by use of the established CMS classifier. As a result, 54 of 62 (87%) adenomas were classified according to the CMS. The CMS3 ‘metabolic subtype’, which was least common among CRCs, was most prevalent among adenomas (n = 45; 73%). One of the two adenomas showing MSI was classified as CMS1 (2%), the ‘MSI immune’ subtype. Eight adenomas (13%) were classified as the ‘canonical’ CMS2. No adenomas were classified as the ‘mesenchymal’ CMS4, consistent with the fact that adenomas lack invasion‐associated stroma. The distribution of the CMS classes among adenomas was confirmed in an independent series. CMS3 was enriched with adenomas at low risk of progressing to CRC, whereas relatively more high‐risk adenomas were observed in CMS2. We conclude that adenomas can be stratified into the CMS classes. Considering that CMS1 and CMS2 expression signatures may mark adenomas at increased risk of progression, the distribution of the CMS classes among adenomas is consistent with the proportion of adenomas expected to progress to CRC. © 2018 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
Activation of sterol regulatory element‐binding protein 1 (SREBP1)‐mediated lipogenesis by the Epstein–Barr virus‐encoded latent membrane protein 1 (LMP1) promotes cell proliferation and progression of nasopharyngeal carcinoma
Authors: Lo Angela Kwok‐Fung , Lung Raymond Wai‐Ming , Dawson Christopher W , Young Lawrence S , Ko Chuen‐Wai , Yeung Walter Wai , Kang Wei , To Ka‐Fai , Lo Kwok‐Wai 
Published: July 2018

AbstractNasopharyngeal carcinoma (NPC) is closely associated with Epstein–Barr virus (EBV) infection. The EBV‐encoded latent membrane protein 1 (LMP1), which is commonly expressed in NPC, engages multiple signaling pathways that promote cell growth, transformation, and metabolic reprogramming. Here, we report a novel function of LMP1 in promoting de novo lipogenesis. LMP1 increases the expression, maturation and activation of sterol regulatory element‐binding protein 1 (SREBP1), a master regulator of lipogenesis, and its downstream target fatty acid synthase (FASN). LMP1 also induces de novo lipid synthesis and lipid droplet formation. In contrast, small interfering RNA (siRNA) knockdown of LMP1 in EBV‐infected epithelial cells diminished SREBP1 activation and lipid biosynthesis. Furthermore, inhibition of the mammalian target of rapamycin (mTOR) pathway, through the use of either mTOR inhibitors or siRNAs, significantly reduced LMP1‐mediated SREBP1 activity and lipogenesis, indicating that LMP1 activation of the mTOR pathway is required for SREBP1‐mediated lipogenesis. In primary NPC tumors, FASN overexpression is common, with high levels correlating significantly with LMP1 expression. Moreover, elevated FASN expression was associated with aggressive disease and poor survival in NPC patients. Luteolin and fatostatin, two inhibitors of lipogenesis, suppressed lipogenesis and proliferation of nasopharyngeal epithelial cells, effects that were more profound in cells expressing LMP1. Luteolin and fatostatin also dramatically inhibited NPC tumor growth in vitro and in vivo. Our findings demonstrate that LMP1 activation of SREBP1‐mediated lipogenesis promotes tumor cell growth and is involved in EBV‐driven NPC pathogenesis. Our results also reveal the therapeutic potential of utilizing lipogenesis inhibitors in the treatment of locally advanced or metastatic NPC. © 2018 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
Surgical management of children and adolescents with upfront completely resected hepatocellular carcinoma
Authors: D'Souza Amber M. , Shah Rachana , Gupta Anita , Towbin Alexander J. , Alonso Maria , Nathan Jaimie D. , Bondoc Alex , Tiao Greg , Geller James I. 
Published: July 2018

AbstractBackgroundHepatocellular carcinoma (HCC) is an aggressive malignant neoplasm that is often chemoresistant. Complete surgical resection remains the mainstay of therapy. The role of liver transplantation (LT) in pediatric HCC is in evolution, as is the role of adjuvant chemotherapy for stage I disease.MethodsA retrospective review of patients < 18 years of age with completely resected HCC treated with surgical intervention alone at our institution from 2004 to 2015 was conducted.ResultsTwelve patients with a median age of 12 years (range = 1–17; number of females = 7) with upfront resected HCC (Evans stage I) were identified. Four patients had HCC without identifiable risk factors (fibrolamellar‐HCC = 2; early HCC arising in focal nodular hyperplasia = 1, well‐differentiated [wd] HCC = 1). Four patients had early or wd‐HCC in the context of portosystemic shunts (Abernethy = 2; mesocaval shunt and portal vein thrombosis = 2). Four patients had moderate to wd‐HCC in the context of pre‐existing liver disease with cirrhosis (progressive familial intrahepatic cholestasis type‐2 = 2, alpha‐1 antitrypsin deficiency = 1, Alagille syndrome = 1). Seven patients underwent LT (multifocal = 5; solitary = 2); five exceeded Milan criteria (MC) by imaging. Five patients underwent complete resection (segmentectomy = 2; hemihepatectomy = 3). Ten patients received no adjuvant chemotherapy. All patients are alive without evidence of disease with a median follow‐up of 54.1 months (range = 28.1–157.7 months).ConclusionsPediatric and adolescent patients with upfront, completely resected HCC can be effectively treated without chemotherapy. LT should be considered for nonmetastatic HCC, especially in the context of pre‐existing chronic liver disease, even when the tumor exceeds MC. Distinct pediatric selection criteria are needed to identify patients most suitable for LT.
Oncofertility—An emerging discipline rather than a special consideration
Authors: Anazodo Antoinette , Ataman‐Millhouse Lauren , Jayasinghe Yasmin , Woodruff Teresa K. 
Published: July 2018

AbstractOriginally absent from the oncologist's consult, then placed in a ‘quality of life’ rubric, oncofertility should now be an essential part of a comprehensive cancer treatment plan in patients of reproductive age, including adolescents and young adults (AYAs). Oncofertility encompasses the endocrine health of the patient, as well as fertility management options. Thus, pubertal transitions in males and females, bone health, and menstrual health are all part of this discipline, enabling practitioners to work in interdisciplinary teams to solve problems in reproductive health. This review provides a summary of the essential considerations required for the assessement of reproductive risk and choice of fertility preservation options as well as considerations for developing oncofertility services for AYAs.
Origins of STIL‐TAL1 fusion genes in children who later developed paediatric T‐cell acute lymphoblastic leukaemia: An investigation of neonatal blood spots
Authors: Gustafsson Britt M. , Mattsson Kristin , Bogdanovic Gordana , Leijonhufvud Gustaf , Honkaniemi Emma , Ramme Kim , Ford Anthony M. 
Published: July 2018

AbstractSCL/TAL1 interrupting locus (STIL)‐T‐cell acute leukaemia (TAL1) fusion genes are present in approximately 11‐27% of children with paediatric T‐cell acute lymphoblastic leukaemia (T‐ALL), but the developmental timing of the rearrangement is still unknown. To investigate whether the fusion gene can be detected in neonatal blood spots (NBSs) from paediatric patients diagnosed with T‐cell ALL, we analysed DNA from 38 paediatric patients with T‐ALL by nested polymerase chain reaction and electrophoresis. The STIL‐TAL1 fusion gene was not detected in NBSs from any of the 38 patients with T‐ALL, suggesting that STIL‐TAL1 fusion genes are most probably postnatal events in paediatric T‐ALL.
A Potential Golden Age to Come—Current Tools, Recent Use Cases, and Future Avenues for De Novo Sequencing in Proteomics
Authors: Muth Thilo , Hartkopf Felix , Vaudel Marc , Renard Bernhard Y. 
Published: July 2018

AbstractIn shotgun proteomics, peptide and protein identification is most commonly conducted using database search engines, the method of choice when reference protein sequences are available. Despite its widespread use the database‐driven approach is limited, mainly because of its static search space. In contrast, de novo sequencing derives peptide sequence information in an unbiased manner, using only the fragment ion information from the tandem mass spectra. In recent years, with the improvements in MS instrumentation, various new methods have been proposed for de novo sequencing. This review article provides an overview of existing de novo sequencing algorithms and software tools ranging from peptide sequencing to sequence‐to‐protein mapping. Various use cases are described for which de novo sequencing was successfully applied. Finally, limitations of current methods are highlighted and new directions are discussed for a wider acceptance of de novo sequencing in the community.
Exchange‐Biased Fe3−xO4‐CoO Granular Composites of Different Morphologies Prepared by Seed‐Mediated Growth in Polyol: From Core–Shell to Multicore Embedded Structures
Authors: Franceschin Giulia , Gaudisson Thomas , Menguy Nicolas , Dodrill Brad C. , Yaacoub Nader , Grenèche Jean‐Marc , Valenzuela Raul , Ammar Souad 
Published: July 2018

AbstractMagnetically contrasted granular hetero‐nanostructures are prepared by seed‐mediated growth in polyol, properly combining two oxide phases with different magnetic order, ferrimagnetic (F) partially oxidized magnetite Fe3−xO4 and antiferromagnetic (AF) cobalt oxide. Spinel Fe3−xO4 nanoparticles are first synthesized and then used as seeds for rock salt CoO nanocrystals growth. Three different hetero‐nanostructure designs are realized, acting on the content ratio between the seeds and the deposit's precursors during the synthesis. For all of them, the spinel and the rock salt phases are confirmed by X‐ray diffraction and high‐resolution transmission electron microscopy. Both phases are obtained in high‐crystalline quality with a net epitaxial relationship between the two crystallographic lattices. Mössbauer spectrometry confirms the cobalt cation diffusion into the spinel seeds, giving favorable chemical interfacing with the rock salt deposit, thus prevailing its heterogeneous nucleation and consequently offering the best condition for exchange‐bias (EB) onset. Magnetic measurements confirm EB features. The overall magnetic properties are found to be a complex interplay between dipolar interactions, exchange anisotropy at the F/AF interface, and magnetocrystalline anisotropy enhancement in the F phase, due to Co2+ diffusion into iron oxide's crystalline lattice. These results underline the powerfulness of colloidal chemistry for functional granular hetero‐nanostructured material processing.