bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospective Validation of an Ex Vivo, Patient-Derived 3D Spheroid Model for Response Predictions in Newly Diagnosed Ovarian Cancer
Authors: Shuford Stephen , Wilhelm Christine , Rayner Melissa , Elrod Ashley , Millard Melissa , Mattingly Christina , Lotstein Alina , Smith Ashley M. , Guo Qi Jin , O’Donnell Lauren , Elder Jeffrey , Puls Larry , Weroha S. John , Hou Xiaonan , Zanfagnin Valentina , Nick Alpa , Stany Michael P. , Maxwell G. Larry , Conrads Thomas , Sood Anil K. , Orr David , Holmes Lillia M. , Gevaert Matthew , Crosswell Howland E. , DesRochers Teresa M. 
Published: August 2019

AbstractAlthough 70–80% of newly diagnosed ovarian cancer patients respond to first-line therapy, almost all relapse and five-year survival remains below 50%. One strategy to increase five-year survival is prolonging time to relapse by improving first-line therapy response. However, no biomarker today can accurately predict individual response to therapy. In this study, we present analytical and prospective clinical validation of a new test that utilizes primary patient tissue in 3D cell culture to make patient-specific response predictions prior to initiation of treatment in the clinic. Test results were generated within seven days of tissue receipt from newly diagnosed ovarian cancer patients obtained at standard surgical debulking or laparoscopic biopsy. Patients were followed for clinical response to chemotherapy. In a study population of 44, the 32 test-predicted Responders had a clinical response rate of 100% across both adjuvant and neoadjuvant treated populations with an overall prediction accuracy of 89% (39 of 44, p < 0.0001). The test also functioned as a prognostic readout with test-predicted Responders having a significantly increased progression-free survival compared to test-predicted Non-Responders, p = 0.01. This correlative accuracy establishes the test’s potential to benefit ovarian cancer patients through accurate prediction of patient-specific response before treatment.
Effect of immunosuppression in miRNAs from extracellular vesicles of colorectal cancer and their influence on the pre-metastatic niche
Authors: Tubita Valeria , Segui-Barber Joan , Lozano Juan José , Banon-Maneus Elisenda , Rovira Jordi , Cucchiari David , Moya-Rull Daniel , Oppenheimer Federico , Del Portillo Hernando , Campistol Josep M. , Diekmann Fritz , Ramirez-Bajo Maria José , Revuelta Ignacio 
Published: August 2019

AbstractColorectal cancer (CRC) occurs with more aggressiveness in kidney transplant recipients compared to the general population. Immunosuppressive therapy plays a crucial role in the development of post-transplant malignancy. Concretely, cyclosporine A (CsA) has intrinsic pro-oncologic properties, while several studies report a regression of cancer after the introduction of rapamycin (RAPA). However, their effect on the extracellular vesicle (EV) content from CRC cell lines and their relevance in the pre-metastatic niche have not yet been studied. Here, we investigated the effect of RAPA and CsA in EV-miRNAs from metastatic and non-metastatic CRC cell lines and the role of relevant miRNAs transferred into a pre-metastatic niche model. EV-miRNA profiles showed a significant upregulation of miR-6127, miR-6746-5p, and miR-6787-5p under RAPA treatment compared to CsA and untreated conditions in metastatic cell lines that were not observed in non-metastatic cells. From gene expression analysis of transfected lung fibroblasts, we identified 22 shared downregulated genes mostly represented by the histone family involved in chromatin organization, DNA packaging, and cell cycle. These results suggest that EV-miR-6127, miR-6746-5p and miR-6787-5p could be a potential epigenetic mechanism induced by RAPA therapy in the regulation of the pre-metastatic niche of post-transplant colorectal cancer.
Comparing human iPSC-cardiomyocytes versus HEK293T cells unveils disease-causing effects of Brugada mutation A735V of NaV1.5 sodium channels
Authors: de la Roche Jeanne , Angsutararux Paweorn , Kempf Henning , Janan Montira , Bolesani Emiliano , Thiemann Stefan , Wojciechowski Daniel , Coffee Michelle , Franke Annika , Schwanke Kristin , Leffler Andreas , Luanpitpong Sudjit , Issaragrisil Surapol , Fischer Martin , Zweigerdt Robert 
Published: August 2019

AbstractLoss-of-function mutations of the SCN5A gene encoding for the sodium channel α-subunit NaV1.5 result in the autosomal dominant hereditary disease Brugada Syndrome (BrS) with a high risk of sudden cardiac death in the adult. We here engineered human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) carrying the CRISPR/Cas9 introduced BrS-mutation p.A735V-NaV1.5 (g.2204C > T in exon 14 of SCN5A) as a novel model independent of patient´s genetic background. Recent studies raised concern regarding the use of hiPSC-CMs for studying adult-onset hereditary diseases due to cells’ immature phenotype. To tackle this concern, long-term cultivation of hiPSC-CMs on a stiff matrix (27–42 days) was applied to promote maturation. Patch clamp recordings of A735V mutated hiPSC-CMs revealed a substantially reduced upstroke velocity and sodium current density, a prominent rightward shift of the steady state activation curve and decelerated recovery from inactivation as compared to isogenic hiPSC-CMs controls. These observations were substantiated by a comparative study on mutant A735V-NaV1.5 channels heterologously expressed in HEK293T cells. In contrast to mutated hiPSC-CMs, a leftward shift of sodium channel inactivation was not observed in HEK293T, emphasizing the importance of investigating mechanisms of BrS in independent systems. Overall, our approach supports hiPSC-CMs’ relevance for investigating channelopathies in a dish.
A miniaturized spectrometer for NMR relaxometry under extreme conditions
Authors: Tang Yiqiao , McCowan David , Song Yi-Qiao 
Published: August 2019

AbstractWith the advent of integrated electronics, microfabrication and novel chemistry, NMR (Nuclear Magnetic Resonance) methods, embodied in miniaturized spectrometers, have found profound uses in recent years that are beyond their conventional niche. In this work, we extend NMR relaxometry on a minute sample below 20 μL to challenging environment of 150 °C in temperature and 900 bar in pressure. Combined with a single-board NMR spectrometer, we further demonstrate multidimensional NMR relaxometries capable of resolving compositions of complex fluids. The confluence of HTHP (high-pressure high-temperature) capability, minimal sample volume, and reduced sensor envelop and power budget creates a new class of mobile NMR platforms, bringing the powerful analytical toolkit in a miniaturized footprint to extreme operating conditions.
β-amyloid pathology and hippocampal atrophy are independently associated with memory function in cognitively healthy elderly
Authors: Svenningsson Anna L. , Stomrud Erik , Insel Philip S. , Mattsson Niklas , Palmqvist Sebastian , Hansson Oskar 
Published: August 2019

AbstractThe independent effects of different brain pathologies on age-dependent cognitive decline are unclear. We examined this in 300 cognitively unimpaired elderly individuals from the BioFINDER study. Using cognition as outcome we studied the effects of cerebrospinal fluid biomarkers for amyloid-β (Aβ42/40), neuroinflammation (YKL-40), and neurodegeneration and tau pathology (T-tau and P-tau) as well as MRI measures of white-matter lesions, hippocampal volume (HV), and regional cortical thickness. We found that Aβ positivity and HV were independently associated with memory. Results differed depending on age, with memory being associated with HV (but not Aβ) in older participants (73.3–88.4 years), and with Aβ (but not HV) in relatively younger participants (65.2–73.2 years). This indicates that Aβ and atrophy are independent contributors to memory variability in cognitively healthy elderly and that Aβ mainly affects memory in younger elderly individuals. With advancing age, the effect of brain atrophy overshadows the effect of Aβ on memory function.
Altered immune parameters associated with Koala Retrovirus (KoRV) and Chlamydial infection in free ranging Victorian koalas (Phascolarctos cinereus)
Authors: Maher Iona E. , Patterson Jade , Curnick Megan , Devlin Joanne , Higgins Damien P. 
Published: August 2019

AbstractKoala Retrovirus (KoRV) has been widely speculated to cause immune suppression in koalas (Phascolarctos cinereus) and to underlie the koala’s susceptibility to infectious disease, however evidence for immunomodulation is limited. The aim of this study is to determine whether immunophenotypic changes are associated with KoRV infection in free ranging Victorian koalas. qPCR was used to examine mRNA expression for Th1 (IFNγ), Th2-promoting (IL6, IL10) and Th17 (IL17A) cytokines, along with CD4 and CD8 in whole blood of koalas (n = 74) from Mt Eccles and Raymond Island in Victoria, Australia, with and without natural chlamydial infection. KoRV positive koalas had significantly lower levels of IL17A (p`0.023) and IFNγ (p = 0.044) gene expression along with a decreased CD4:CD8 gene expression ratio (p = 0.025) compared to negative koalas. No effect of chlamydial infection or combined effect of KoRV and chlamydial infection was detected in these populations. The decreased expression of IFNγ could make KoRV infected koalas more susceptible to persistent chlamydial infection, and a decrease in IL17A could make them more susceptible to gram negative bacterial, fungal and mycobacterial infection; but more tolerant of chlamydial infection.
Sexually dimorphic leanness and hypermobility in p16Ink4a/CDKN2A-deficient mice coincides with phenotypic changes in the cerebellum
Authors: Kim Kwang H. , Cho Yejin , Lee Jaehoon , Jeong Haengdueng , Lee Yura , Kim Soo In , Kim Chang-Hoon , Lee Han-Woong , Nam Ki Taek 
Published: August 2019

Abstractp16Ink4a/CDKN2A is a tumor suppressor that critically regulates the cell cycle. Indeed, p16Ink4a deficiency promotes tumor formation in various tissues. We now report that p16Ink4a deficiency in female mice, but not male mice, induces leanness especially in old age, as indicated by lower body weight and smaller white adipose tissue, although other major organs are unaffected. Unexpectedly, the integrity, number, and sizes of adipocytes in white adipose tissue were unaffected, as was macrophage infiltration. Hence, hypermobility appeared to be accountable for the phenotype, since food consumption was not altered. Histological analysis of the cerebellum and deep cerebellar nuclei, a vital sensorimotor control center, revealed increased proliferation of neuronal cells and improved cerebellum integrity. Expression of estrogen receptor β (ERβ) and PCNA also increased in deep cerebellar nuclei, implying crosstalk between p16Ink4a and ERβ. Furthermore, p16Ink4a deficiency expands LC3B+ cells and GFAP+ astrocytes in response to estrogen. Collectively, the data suggest that loss of p16INK4a induces sexually dimorphic leanness in female mice, which appears to be due to protection against cerebellar senescence by promoting neuronal proliferation and homeostasis via ERβ.
CrI3-WTe2: A Novel Two-Dimensional Heterostructure as Multisensor for BrF3 and COCL2 Toxic Gases
Authors: Bano Amreen , Krishna Jyoti , Maitra Tulika , Gaur N. K. 
Published: August 2019

AbstractA new multisensor (i.e. resistive and magnetic) CrI3-WTe2 heterostructure (HS) to detect the toxic gases BrF3 and COCl2 (Phosgene) has been theoretically studied in our present investigation. The HS has demonstrated sensitivity towards both the gases by varying its electronic and magnetic properties when gas molecule interacts with the HS. Fast recovery time (<0.14 fs) under UV radiation has been observed. We have considered two configurations of BrF3 adsorbed HS; (1) when F ion interacts with HS (C1) and (2) when Br ion interacts with HS (C2). In C1 case the adsorption energy Ead is observed to be −0.66 eV while in C2 it is −0.95 eV. On the other hand in case of COCl2Ead is found to be −0.42 eV. Magnetic moments of atoms are also found to vary upon gas adsorption indicates the suitability of the HS as a magnetic gas sensor. Our observations suggest the suitability of CrI3-WTe2 HS to respond detection of the toxic gases like BrF3 and COCl2.
Hyperbolic plasmon–phonon dispersion on group velocity reversal and tunable spontaneous emission in graphene–ferroelectric substrate
Authors: Debu Desalegn T. , Ladani Faezeh Tork , French David , Bauman Stephen J. , Herzog Joseph B. 
Published: August 2019

AbstractThis work presents a new substrate platform, which provides tunability of the group velocity and spontaneous emission of a dipolar scatterer graphene–ferroelectric slab hybrid system in the terahertz ranges. We use analytical models to determine the hybridization of graphene surface plasmon and ferroelectric LiNbO3 type I and type II reststrahlen hyperbolic phonon–polariton. The variation of the chemical potential of graphene and the thickness of the ferroelectric layer results in several distinct features. Flipping the group velocity, strongly coupled hybrid hyperbolic surface plasmon–polaritons, and surface plasmon–polariton mode exists for the same momentum at different frequencies. The group velocity sign reversal for both a single-graphene- and double-graphene-integrated system depends on the thickness of the hyperbolic layer and the chemical potential of graphene. Comparative analysis of Purcell radiation is presented for a quantum emitter positioned at different locations between ferroelectric and graphene-integrated ferroelectric layers, revealing that this system can support strong spontaneous emission that can be modulated with the graphene chemical potential. Changing the chemical potential through selective voltage biasing demonstrates a substantial increase or decrease in the decay rate for spontaneous emission. Further analysis of the emission phenomenon shows a dependence on factors, such as the relative radiating source position and the thickness of the ferroelectric film. These characteristics make graphene–ferroelectric materials promising candidates to modify the light–matter interaction at the low terahertz ranges.
The roles of reactive oxygen species and antioxidants in cryopreservation
Authors: Len Jia Soon , Koh Wen Shuo Darius , Tan Shi-Xiong 
Published: August 2019

AbstractCryopreservation has facilitated advancement of biological research by allowing the storage of cells over prolonged periods of time. While cryopreservation at extremely low temperatures would render cells metabolically inactive, cells suffer insults during the freezing and thawing process. Among such insults, the generation of supra-physiological levels of reactive oxygen species (ROS) could impair cellular functions and survival. Antioxidants are potential additives that were reported to partially or completely reverse freeze-thaw stress-associated impairments. This review aims to discuss the potential sources of cryopreservation-induced ROS and the effectiveness of antioxidant administration when used individually or in combination.
Equivalent circuit model of an ultra‐wideband frequency selective surface composite absorbing material
Authors: Yu Jiaao , Peng Shirui , Nan Hao , Liu Jianhao 
Published: August 2019

An equivalent circuit model of double hexagonal loops frequency selective surface (FSS) was established. The formulas of LC parameters were proposed with equivalent repetition period. The reflection frequency, transmission frequency, and the location of first modal interaction null were simulated, and the results showed a good agreement in full‐wave simulation result. On this basis, an ultra‐wideband composite absorbing material (CAM) was proposed, and its equivalent circuit model was also established. A sample was fabricated and measured to verify the validity of the model. The results showed a good ultra‐wideband absorbing performance in the range of 1.9–8.9 GHz.
Proximity‐coupled feed patch antenna array for 79 GHz automotive radar
Authors: Tian Haiyan , Liu Changjiang , Gu Xiang 
Published: August 2019

Autonomous driving is becoming a reality‐but low‐cost 79 GHz radar antenna technology is still imperfect. Here, the authors propose a kind of proximity‐coupled feed patch antenna array. By reducing the non‐linear mutual interference between the transmission line and the radiating elements, the antenna's operating bandwidth is improved and the antenna gain is increased. Moreover, the consistency of the antenna radiation pattern at different frequencies is also improved. The simulation results show that the antenna has a −10 dB impedance bandwidth from 76 to 81.4 GHz, and the gain is >14.8 dB. Within the operating frequency band, the antenna main lobe width is smaller than ±1.2°
Assessment and Management of Infection in Alcoholic Hepatitis
Authors: Vergis Nikhil , Atkinson Stephen R. , Thursz Mark R. 
Published: August 2019

AbstractSevere alcoholic hepatitis (SAH) is a condition characterized by jaundice and liver failure that develops after heavy and prolonged alcohol consumption. Infection frequently complicates the natural history of the disease and is independently associated with mortality. Objective recognition and recording of infection are therefore essential in the evaluation of therapeutic interventions and for antibiotic stewardship. This review will evaluate infections that complicate SAH at admission and beyond. Factors that associate with the development of infection will be identified and clinical and laboratory techniques available to identify infection will be discussed. Common pathogens and frequently used antibiotics will be reviewed and recommendations will be made for the management of infection for SAH patients. New techniques to assess infection earlier and more precisely may improve diagnosis and treatment of this important driver of mortality in SAH.
Platelet PI3K Modulates Innate Leukocyte Extravasation during Acid-Induced Acute Lung Inflammation
Authors: Kral-Pointner Julia Barbara , Schrottmaier Waltraud Cornelia , Salzmann Manuel , Mussbacher Marion , Schmidt Georg Johannes , Moser Bernhard , Heber Stefan , Birnecker Birgit , Paar Hannah , Zellner Maria , Knapp Sylvia , Assinger Alice , Schabbauer Gernot 
Published: August 2019

Introduction Blood platelets are increasingly recognized as modulators of leukocyte effector functions in various pathologies including acute lung injury (ALI). ALI is a life-threatening disease, caused by damage to the alveolar epi- and endothelium. Excessive accumulation of leukocytes leads to severe lung inflammation, resulting in impaired lung function and hypoxemia.
Objective Since leukocyte migration is modulated by activated platelets and phosphatidylinositol 3-kinase (PI3K) signaling is involved in platelet function, we aimed to elucidate the effect of PI3K on platelet-mediated immune responses.
Materials and Methods We generated a mouse model with a platelet-specific deletion of p85α, the most important regulatory subunit of the class IA PI3K, and evaluated platelet function and platelet–leukocyte interactions. Moreover, we analyzed the impact of platelet-specific p85α gene deficiency during sterile peritonitis and acid-induced ALI.
Results In vitro analyses of platelets revealed that lack of p85α led to decreased downstream signaling and diminished expression of surface activation markers, for example, CD62P and CD63, as well as reduced platelet aggregation. Moreover, platelet PI3K essentially mediated direct interactions of platelets with monocytes and neutrophils. In mice, platelet-specific p85α deficiency prevented leukocyte infiltration into the peritoneum and the bronchoalveolar compartment during sterile peritonitis and ALI, respectively. Additionally, the release of the inflammatory cytokine interleukin-12/23 was diminished in platelet p85α-deficient mice during ALI. In contrast to PI3K, neither overexpression nor depletion of platelet phosphatase and tensin homolog, the endogenous antagonist of PI3K, significantly modulated platelet function.
Conclusion Our data indicate a crucial role of platelet PI3K signaling for leukocyte extravasation upon inflammatory stimuli in various diseases models.
Rapid and active stabilization of visual cortical firing rates across light–dark transitions
Authors: Torrado Pacheco Alejandro , Tilden Elizabeth I. , Grutzner Sophie M. , Lane Brian J. , Wu Yue , Hengen Keith B. , Gjorgjieva Julijana , Turrigiano Gina G. 
Published: August 2019

Significance
The firing dynamics of neurons in primary visual cortex (V1) are poorly understood. Indeed, V1 neurons of freely behaving rats fire at the same mean rate in light and darkness. It is unclear how this stability is maintained and whether it is important for sensory processing. We find that transitions between light and darkness happening at expected times have only modest effects on V1 activity. In contrast, both unexpected transitions and light reexposure after extended darkness robustly increase V1 firing. Finally, pairwise correlations in neuronal spiking are significantly higher during the light when natural vision is occurring. These data show that V1 firing rates are actively stabilized while simultaneously allowing for input-dependent changes in correlations between neurons.
The BINGO telescope: a new instrument exploring the new 21 cm cosmology window
Authors: Wuensche C.A. 
Published: August 2019

Abstract
BINGO is a unique radio telescope designed to make the first detection of Baryon Acoustic Oscillations (BAO) at radio frequencies. This will be achieved by measuring the distribution of neutral hydrogen gas at cosmological distances using a technique called Intensity Mapping. Along with the Cosmic Microwave Background anisotropies, the scale of BAO is one of the most powerful probes of cosmological parameters, including dark energy. The telescope will be built in a very low RFI site in South America and will operate in the frequency range from 0.96 GHz to 1.26 GHz. The telescope design consists of two ∼ 40-m compact mirrors with no moving parts. Such a design will give the excellent polarization performance and very low sidelobe levels required for intensity mapping. With a feedhorn array of 50 receivers, it will map a 15° declination strip as the sky drifts past the field-of-view of the telescope. The BINGO consortium is composed Universidade de Sao Paulo, Instituto Nacional de Pesquisas Espaciais (Brazil), University of Manchester and University College London (United Kingdom), ETH Zurich (Switzerland) and Universidad de La Republica (Uruguay). The telescope assembly and horn design and fabrication are under way in Brazil. The receiver was designed in UK and will be developed in Brazil, with most of the components for the receiver will also be supplied by Brazilian industry. The experience and science goals achieved by the BINGO team will be advantageous as a pathfinder mission for the Square Kilometre Array (SKA) project. This paper reports the current status of the BINGO mission, as well as preliminary results already obtained for the instrumentation development.
A scheduler for the National Aures Observatory
Authors: Bekhti Y A , Lamraoui K , Seghouani N , Benatchba K , Bouzidi-Hassini S 
Published: August 2019

Abstract
The main project for National Aures Observatory (NAO) is the construction of a Meta-Telescope consisting of several robotic and ultra-rapid telescopes. This telescope ‘farm’ will face hundreds of observation requests that have to be scheduled. The goal of this work is to compute the best schedule for all observations, over all night and using all available telescopes. The scheduler must also take into account all the interruptions that may randomly occur during the observation process, especially those relative to Gravitational Waves and Gamma-Ray Bursts alerts, which are the main goal of the Aures Observatory. To solve this multi-objectives optimization problem, we proposed a scheduler of a predictive class. A genetic algorithm approach was used to compute the solution of the scheduling problem based on NSGA-II and using Pareto Optimality.