bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Aspartic Protease Ddi1 Contributes to DNA-Protein Crosslink Repair in Yeast
Authors: Serbyn Nataliia , Noireterre Audrey , Bagdiul Ivona , Plank Michael , Michel Agnès H , Loewith Robbie , Kornmann Benoît , Stutz Françoise 
Published: March 2019

SUMMARYNaturally occurring or drug-induced DNA-protein crosslinks (DPCs) interfere with key DNA transactions if not timely repaired. The unique family of DPC-specific proteases Wss1/SPRTN targets DPC protein moieties for degradation, including topoisomerase-1 trapped in covalent crosslinks (Top1ccs). Here we describe that the efficient DPC disassembly requires Ddi1, another conserved predicted protease in Saccharomyces cerevisiae. We found Ddi1 in a genetic screen of the tdp1wss1 mutant defective in Top1cc processing. Ddi1 is recruited to a persistent Top1cc-like DPC lesion in an S-phase dependent manner to assist eviction of crosslinked protein from DNA. Loss of Ddi1 or its putative protease activity hypersensitize cells to DPC trapping agents independently from Wss1 and 26S proteasome, implying its broader role in DPC repair. Among potential Ddi1 targets we found the core component of RNAP II and show that its genotoxin-induced degradation is impaired in ddi1. Together, we propose that the Ddi1 protease contributes to DPC proteolysis.
Decentralized Distribution-sampled Classification Models with Application to Brain Imaging
Authors: Lewis Noah , Gazula Harshvardhan , Plis Sergey M. , Calhoun Vince D. 
Published: March 2019

Abstract0.1backgroundIn this age of big data, large data stores allow researchers to compose robust models that are accurate and informative. In many cases, the data are stored in separate locations requiring data transfer between local sites, which can cause various practical hurdles, such as privacy concerns or heavy network load. This is especially true for medical imaging data, which can be constrained due to the health insurance portability and accountability act (HIPAA). Medical imaging datasets can also contain many thousands or millions of features, requiring heavy network load.0.2New MethodOur research expands upon current decentralized classification research by implementing a new singleshot method for both neural networks and support vector machines. Our approach is to estimate the statistical distribution of the data at each local site and pass this information to the other local sites where each site resamples from the individual distributions and trains a model on both locally available data and the resampled data.0.3ResultsWe show applications of our approach to handwritten digit classification as well as to multi-subject classification of brain imaging data collected from patients with schizophrenia and healthy controls. Overall, the results showed comparable classification accuracy to the centralized model with lower network load than multishot methods.0.4Comparison with Existing MethodsMany decentralized classifiers are multishot, requiring heavy network traffic. Our model attempts to alleviate this load while preserving prediction accuracy.0.5ConclusionsWe show that our proposed approach performs comparably to a centralized approach while minimizing network traffic compared to multishot methods.0.6HighlightsA novel yet simple approach to decentralized classificationReduces total network load compared to current multishot algorithmsMaintains a prediction accuracy comparable to the centralized approach
Pore-modulating toxins exploit inherent slow inactivation to block K+channels
Authors: Karbat Izhar , Altman-Gueta Hagit , Fine Shachar , Szanto Tibor , Hamer-Rogotner Shelly , Dym Orly , Frolow Felix , Gordon Dalia , Panyi Gyorgy , Gurevitz Michael , Reuveny Eitan 
Published: March 2019

AbstractVoltage dependent potassium channels (Kvs) gate in response to changes in electrical membrane potential by coupling a voltage-sensing module with a K+-selective pore. Animal toxins targeting Kvs are classified to “pore-blockers” that physically plug the ion conduction pathway and “gating modifiers” that disrupt voltage sensor movements. A third group of toxins blocks K+conduction by an unknown mechanism via binding to the channel turrets. Here we show that Cs1, a peptide toxin isolated from cone snail venom, binds at the turrets of Kv1.2 and targets a network of hydrogen bonds that govern water access to the peripheral cavities that surround the central pore. The resulting ectopic water flow triggers an asymmetric collapse of the pore by a process resembling that of inherent slow inactivation. Pore modulation by animal toxins exposes the peripheral cavity of K+channels as a novel pharmacological target and provides a rational framework for drug design.
Accessible and interactive RNA sequencing analysis using serverless computing
Authors: Hung Ling-Hong , Niu Xingzhi , Lloyd Wes , Yeung Ka Yee 
Published: March 2019

AbstractWe present a novel method that yields 1100-fold computational speedup and allows biomedical scientists to interactively adjust alignment parameters in real time to iteratively improve final analytical results. Specifically, the alignment time for a 640 million read human RNA-sequencing dataset is reduced from 19 hours to 1 minute using serverless cloud computing. We provide a graphical interface for the accelerated workflows, thus making our methodology accessible to non-cloud experts.
A Bayesian Approach for Inferring the Impact of a Discrete Character on Rates of Continuous-Character Evolution in the Presence of Background-Rate Variation
Authors: May Michael R. , Moore Brian R. 
Published: March 2019

AbstractUnderstanding how and why rates of character evolution vary across the Tree of Life is central to many evolutionary questions; e.g., does the trophic apparatus (a set of continuous characters) evolve at a higher rate in fish lineages that dwell in reef versus non-reef habitats (a discrete character)? Existing approaches for inferring the relationship between a discrete character and rates of continuous-character evolution rely on comparing a null model (in which rates of continuous-character evolution are constant across lineages) to an alternative model (in which rates of continuous-character evolution depend on the state of the discrete character under consideration). However, these approaches are susceptible to a “straw-man” effect: the influence of the discrete character is inflated because the null model is extremely unrealistic. Here, we describe MuSSCRat, a Bayesian approach for inferring the impact of a discrete trait on rates of continuous-character evolution in the presence of alternative sources of rate variation (“background-rate variation”). We demonstrate by simulation that our method is able to reliably infer the degree of state-dependent rate variation, and show that ignoring background-rate variation leads to biased inferences regarding the degree of state-dependent rate variation in grunts (the fish group Haemulidae). [continuous-character evolution; discrete-character evolution; Bayesian phylogenetic comparative methods; data augmentation]
Coordinated development of the mouse extrahepatic bile duct: implications for neonatal susceptibility to biliary injury
Authors: Khandekar Gauri , Llewellyn Jessica , Kriegermeier Alyssa , Waisbourd-Zinman Orith , Johnson Nicolette , Du Yu , Giwa Roquibat , Liu Xiao , Kisseleva Tatiana , Russo Pierre A. , Theise Neil D. , Wells Rebecca G. 
Published: March 2019

AbstractBackground & AimsThe extrahepatic bile duct is the primary tissue initially affected by the cholangiopathy biliary atresia. Biliary atresia affects neonates exclusively and current animal models suggest that the developing bile duct is uniquely susceptible to damage. In this study, we aimed to define the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts.MethodsWe studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes and isolated extrahepatic bile ducts, and a collagen reporter mouse. Methods included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.ResultsThe cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes. Functional studies showed that the glycocalyx protected against bile acid injury and that neonatal cholangiocyte monolayers were more permeable than adult monolayers. In adult ducts, the submucosal space was filled with collagen I, elastin, hyaluronic acid, and proteoglycans. In contrast, the neonatal submucosa had little collagen I and elastin, although both increased rapidly after birth. In vitro modeling suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile. A Col-GFP reporter mouse showed that cells in the neonatal but not adult submucosa were actively producing collagen.ConclusionWe identified four key differences between the neonatal and adult extrahepatic bile duct. We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.Lay SummaryBiliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction with resulting liver injury. We identify four key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.HighlightsThe apical glycocalyx is thin and patchy in neonatal compared to adult cholangiocytesNeonatal cholangiocytes have immature cell-cell junctions and increased permeabilityThe neonatal submucosal space has minimal collagen I or elastinThe neonatal submucosal space contains many actively collagen-secreting cellsGraphical abstract
The association between dietary habits and periodontal disease in young adult women
Authors: Hosoda Akemi , Komagamine Yuriko , Kanazawa Manabu , Hama Yohei , Kojo Akiko , Minakuchi Shunsuke 
Published: March 2019

AbstractBackgroundAmong middle-aged and elderly individuals, dietary habits have previously been reported to differ between patients with and without periodontal disease. However, in young adults, there are only a few reports that show a correlation between nutrient/food intake and periodontal disease. Moreover, no report has assessed the correlation between dietary habits measured by a self-administered diet history questionnaire (DHQ) and periodontal disease. Therefore, we assessed the correlation between dietary habits, determined using a DHQ, and periodontal disease in young adult women who are likely to develop a periodontal disease.MethodsThe participants were 120 healthy, non-smoking, female college students (mean age, 20.4 ± 1.1 years) from two universities who did not have any systemic disease. The participants were assessed for periodontal disease according to community periodontal index (CPI) and were divided into two groups. Subjects with a CPI code of 0, 1, or 2 were assigned to non-periodontal disease group (non-PD), and subjects with a CPI code of 3 or 4 were assigned to periodontal disease group (PD). Dietary habits were assessed using a DHQ. In addition, physical status, level of difficulty in chewing food (dietary hardness), masticatory performance, and quality of life (QoL) were assessed.ResultsThe PD group had a significantly lower nutrient intake of minerals, fat, water-soluble vitamins, and dietary fiber than the non-PD group. In terms of food groups, the PD group consumed significantly lesser amounts of green and yellow vegetables than the non-PD group. In addition, the PD group consumed significantly lesser amounts of hard foods than the non-PD group.ConclusionYoung adult women with a periodontal disease had a significantly lower nutrient/food intake than young adult women without a periodontal disease.
Effect of sodium molybdate and sodium tungstate in concrete rebar corrosion
Authors: Ahmad Altaf , Kumar Ranveer , Kumar Anil 
Published: March 2019

PurposeThis paper aims to identify an inhibitor to protect rebar corrosion in concrete.Design/methodology/approachThe authors use the simple method of polarization and calculate the change in open-circuit potential and corrosion current density.FindingsSodium molybdate is an efficient inhibitor compared with sodium tungstate for rebar corrosion in concrete.Research limitations/implicationsThis paper has limitation of 0.0001 M concentration of inhibitors for 400 days of exposure in 3.5 per cent sodium chloride solution.Originality/valueThe research focused on the concentration of both inhibitors in the range from 0.1 to 0.0001 M, which resulted in greater structural protection from corrosion in adverse conditions, such as coastal areas.
How selection attributes affect golfers’ satisfaction: a meta-analysis
Authors: Kim Hyun-Duck , Cruz Angelita Bautista 
Published: March 2019

Purpose
The purpose of this paper is to summarize the results of existing studies that addressed the relationship between the selection attributes of golf businesses and golfers’ satisfaction in South Korea.

Design/methodology/approach
Studies on the relationship between selection attributes and satisfaction per golf facility type were retrieved from the Korean Citation Index. Using Comprehensive Meta-Analysis version 2, the effect sizes (ESs) for the following relationships were calculated: between selection attributes and satisfaction with golf facilities in general; between selection attributes and satisfaction per distinct golf facility types (outdoor golf courses vs screen golf facilities); and between the sub-factors of selection attributes and satisfaction per golf facility.

Findings
Medium ESs were found between selection attributes and satisfaction with golf facilities in general (0.394), outdoor golf courses (0.336) and screen golf facilities (0.370). The choice attribute factors of accessibility, employees and concession had large effects on golfers’ satisfaction with outdoor golf courses, while services and concession had large effects on golfers’ satisfaction with screen golf facilities.

Originality/value
Among many Asian countries, golf is extremely popular and attracts more business than other sports; however, changes in consumer viewpoints and business trends necessitate consistent refinement. This meta-analytic approach offered a macro perspective on how golf facility administrators might sustain and develop their businesses by highlighting what choice attributes matter most to golf consumers.
Pawnbroking in pre-1949 China: soft strategies for overcoming a negative image
Authors: Yan Tony , Hyman Michael R. 
Published: March 2019

Purpose
The purpose of this paper is to explore the means for enhancing the image and business legitimacy of a socially discredited industry – pawnbroking in pre-1949 China – are explored. Previous studies suggest companies operating within such industries cannot solely rely on hard marketing strategies “to maximize sales and profits as they do with soaps and shoes” (Davidson, 2003, p. 7). Instead, they must find soft strategies for improving company and industry image and legitimacy.

Design/methodology/approach
This research relies on qualitative analysis of historical data and documents.

Findings
Soft strategies deployed by Chinese pawnbrokers – such as interpretations, moral value advocacies and institutionalized arrangements – contributed substantially to improving pawnbroking’s image and business legitimacy.

Research limitations/implications
Interconnections among ethical values, image, business legitimacy and select marketing strategies are clarified. The efficacy of historically analyzing previously implemented business strategies and their embedding contexts is discussed.

Practical implications
Strategies Chinese pawnbrokers used to mitigate their previously negative image and boost their business legitimacy suggest strategies current socially disapproved companies can use to improve their image and business legitimacy.

Originality/value
A historical analysis of pre-1949 Chinese pawnbroking can suggest soft marketing strategies for overcoming consumers’ negative company and industry impressions.
Do consumers like food product innovation? An analysis of willingness to pay for innovative food attributes
Authors: Nazzaro Concetta , Lerro Marco , Stanco Marcello , Marotta Giuseppe 
Published: March 2019

Purpose
The purpose of this paper is to investigate consumer’s acceptance toward product innovation in the agri-food sector, uncovering consumers’ characteristics able to encourage food innovation acceptance.

Design/methodology/approach
The analysis was carried out by administering a web-based structured questionnaire to a convenient sample of 443 Italian consumers. The study relies on consumers’ willingness to pay (WTP) to assess consumers’ acceptance toward the innovative product, while the Food-Related Lifestyle scale was applied to perform a cluster analysis aiming at detecting the attitude of consumers toward innovations in a traditional food.

Findings
The study findings showed a clear openness of consumers toward product innovation. Indeed, consumers’ WTP for the innovative product was far higher than the traditional one. Further, two out of three consumers’ groups detected (i.e. pro-innovation and rational adopters) exhibit a broad correlation between the innovative product attributes and consumers’ psychographics characteristics, revealing the existence of a large number of potential consumers.

Originality/value
The contribution of the paper to the current literature is twofold. First, it focused on an emerging topic for the agri-food sector (i.e. product innovation) whereby research works are still scarce. Second, product innovation was addressed toward a traditional food that is mostly reluctant to innovation due to consumers’ resistance and skepticism.
Investor sentiment, market competition and trade credit supply
Authors: Huang Hongbin , Li Ran , Bai Ya 
Published: March 2019

PurposeThe purpose of this paper is to study the influence of investor sentiment on the supply of trade credit, and further explores the difference of the effect of investor sentiment on the supply of trade credit in the environment of strong market competition and weak market competition.Design/methodology/approachThe authors use panel estimation techniques to examine the impact of investor sentiment in the Chinese securities market on the supply of corporate trade credit.FindingsThis paper finds that investor sentiment has positive impact on trade credit through three channels of motivation, willingness and ability. At the same time, this paper finds that investor sentiment has stronger impact on enterprises in strong market competition than enterprises in weak market competition.Research limitations/implicationsThis paper expands the research on the influence of virtual economy on the real economy, analyzes the difference of the influence of investor sentiment on the supply of trade credit under different market competition conditions.Practical implicationsThe paper perfects the mechanism of trade credit decision-making at this stage, and provides more evidence for the virtual economy to act on the real economy.Social implicationsThis paper provides a theoretical basis for the government functional departments to use the investor sentiment to play a positive role in trade credit to improve the market competition and guide the development of China’s capital market in the direction of rationalization and health.Originality/valueIn combination with market competition environment and industry characteristics, this paper investigates external irrational factors and studies how investor sentiment affects trade credit supply.
Perceived workplace gender-bias and psychological impact
Authors: Lekchiri Siham , Crowder Cindy , Schnerre Anna , Eversole Barbara A.W. 
Published: March 2019

Purpose
The purpose of this paper is to explore the experiences of working women in a male-dominated country (Morocco) and unveil the unique challenges and everyday gender-bias they face, the psychological impact of the perceived gender-bias and, finally, identify a variety of coping strategies or combatting mechanisms affecting their motivation and retention in the workplace.

Design/methodology/approach
Empirical evidence was obtained using a qualitative research method. The Critical Incident Technique (CIT) was used to collect incidents recalled by women in the select institution reflecting their perceptions of their managers’ ineffective behaviors towards them and the impact of these behaviors. The critical incidents were inductively coded, and behavioral statements were derived from the coded data.

Findings
The qualitative data analysis led them to structure the data according to two theme clusters: The perceived gender-bias behaviors (Covert and evident personal and organizational behaviors) and Psychological impacts resulting from the perceived bias. These behavioral practices included abusive behaviors, unfair treatment, bias and lack of recognition. The psychological impact elements involved decreased productivity, depression, anxiety and low self-esteem.

Practical implications
Understanding these experiences can facilitate the identification of strategies geared towards the retention of women in the workforce, and Moroccan organizations can develop and implement strategies and policies that are geared towards eliminating gender-bias in the workplace and to retaining and motivating women who remain ambitious to work in male-dominated environments and cultures.

Originality/value
This paper provides evidence that sufficient organizational mechanisms to support women in male-dominated environments are still unavailable, leaving them to find the proper coping mechanisms to persevere and resist.
Effects of customer perceptions in multichannel retail banking
Authors: Bravo Rafael , Martínez Eva , Pina José Miguel 
Published: March 2019

Purpose
This paper focuses on the multichannel strategy in the banking sector and its effects on customer engagement. Specifically, the purpose of this paper is to propose a model in which customers’ perceptions of offline and online channels are related to brand trust and brand commitment, which ultimately lead to customer engagement.

Design/methodology/approach
An empirical study was carried out on a sample of 306 individuals and data were analysed through partial least squares.

Findings
The results show that offline experience is more important than online experience in terms of impact on trust and commitment, which are closely linked to customer engagement. Online experience does not have a significant direct influence on brand commitment and its effect on brand trust is moderated by the customer’s familiarity with the channel.

Originality/value
These findings contribute to the advance in the current knowledge of the joint role of online and offline channels with the aim of strengthening customer relationships. From a managerial viewpoint, customer perceptions formed by their experiences in bank branches are more important than customer perceptions of the website’s performance in the explanation of trust and commitment.