bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication and evaluation of structural film adhesive using oxazolidinone modified novolac epoxy resin
Authors: Pal Ranajit , Sudhi Suraj , Raghavan Rajeev 
Published: January 2019

ABSTRACTEpoxy film adhesives are of prime importance for the fabrication of lightweight honeycomb structures for aerospace industries. This work involves the synthesis of oxazolidinone modified epoxy novolac resin (EPN‐OXA) via the reaction of EPN and toluene diisocyanate. EPN‐OXA was characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and epoxy equivalent weight. EPN‐OXA was blended with solid epoxy resin, polyethersulfone (PES) toughened liquid epoxy resin, dicyandiamide, and aluminum powder to fabricate a film adhesive curing at 170–180 °C. Effect of additives and curative on the adhesive property was studied to optimize the composition. Effect of PES on the optimized composition was studied in detail. The best composition exhibited lap shear strength of 370 ± 10 kgf cm−2 at 25 °C and the strength was retained to 75% at −196 °C and 52% at 120 °C. PES significantly enhanced the interfacial strength at different temperatures (~1.6‐fold at −196 and 25 °C and ~1.8‐fold at 120 °C). It also improved tensile strength and fracture toughness by 1.4‐ and 2‐fold, respectively. The toughening effect of PES was further confirmed by scanning electron microscopy images. PES marginally reduced the glass‐transition temperature and it exhibited no effect on thermal stability. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47520.
Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology
Authors: de Groot Simon C. , Sliedregt Karen , van Benthem Peter Paul G. , Rivolta Marcelo N. , Huisman Margriet A. 
Published: January 2019

ABSTRACTIn recent years, there has been an increased interest in stem cells for the purpose of regenerative medicine to deliver a wide range of therapies to treat many diseases. However, two‐dimensional cultures of stem cells are of limited use when studying the mechanism of pathogenesis of diseases and the feasibility of a treatment. Therefore, research is focusing on the strengths of stem cells in the three‐dimensional (3D) structures mimicking organs, that is, organoids, or organ‐on‐chip, for modeling human biology and disease. As 3D technology advances, it is necessary to know which signals stem cells need to multiply and differentiate into complex structures. This holds especially true for the complex 3D structure of the inner ear. Recent work suggests that although other factors play a role, the extracellular matrix (ECM), including its topography, is crucial to mimic a stem cell niche in vitro and to drive stem cells toward the formation of the tissue of interest. Technological developments have led to the investigation of biomaterials that closely resemble the native ECM. In the fast forward moving research of organoids and organs‐on‐chip, the inner ear has hardly received attention. This review aims to provide an overview, by describing the general context in which cells, matrix and morphogens cooperate in order to build a tissue, to facilitate research in 3D inner ear technology. Anat Rec, 303:408–426, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.
Identification of specific UGT1A9‐mediated glucuronidation of licoricidin in human liver microsomes
Authors: Cho Pil Joung , Kim Ju‐Hyun , Lee Hye Suk , Kim Jeong Ah , Lee Sangkyu 
Published: January 2019

AbstractLicoricidin is a major prenylated isoflavone of Glycyrrhiza uralensis Fisch. (Leguminosae), and its pharmacological effects have been reported frequently. Typically, flavonoids having multiple hydroxyl groups are unambiguous substrates for glucuronyl conjugation by UDP‐glucuronosyltransferases (UGTs). The pharmacological effects of flavonoids are derived from the conjugation of glucuronide to yield the bioactive metabolite. Here, the metabolism of licoricidin in pooled human liver microsomes (HLMs) was investigated using high‐resolution quadrupole‐orbitrap mass spectrometry. One metabolite (M1) was identified in HLMs after incubation with licoricidin in the presence of uridine 5′‐diphosphoglucuronic acid (UDPGA) and NADPH. The structure of M1 was determined as a monoglucuronyl licoricidin, which was selectively produced by UGT1A9. Licoricidin showed a higher metabolic ratio and rapid metabolism with the recombinant human UGT1A9 than mycophenolic acid, a well‐known UGT1A9 substrate. In conclusion, the selective formation of 7‐glucuronyl licoricidin by UGT1A9 in HLMs could serve as a new selective substrate to determine the activity of UGT1A9 in vitro.
Application of data mining approach to identify drug subclasses based on solubility and permeability
Authors: Gatarić Biljana , Parojčić Jelena 
Published: January 2019

AbstractSolubility and permeability are recognized as key parameters governing drug intestinal absorption and represent the basis for biopharmaceutics drug classification. The Biopharmaceutics Classification System (BCS) is widely accepted and adopted by regulatory agencies. However, currently established low/high permeability and solubility boundaries are the subject of the ongoing scientific discussion. The aim of the present study was to apply data mining analysis on the selected drugs data set in order to develop a human permeability predictive model based on selected molecular descriptors, and to perform data clustering and classification to identify drug subclasses with respect to dose/solubility ratio (D/S) and effective permeability (Peff). The Peff values predicted for 30 model drugs for which experimental human permeability data are not available were in good agreement with the reported fraction of drug absorbed. The results of clustering and classification analysis indicate the predominant influence of Peff over D/S. Two Peff cut‐off values (1 × 10−4 and 2.7 × 10−4 cm/s) have been identified indicating the existence of an intermediate group of drugs with moderate permeability. Advanced computational analysis employed in the present study enabled the recognition of complex relationships and patterns within physicochemical and biopharmaceutical properties associated with drug bioperformance.
Comprehensive mutation profiling and mRNA expression analysis in atypical chronic myeloid leukemia in comparison with chronic myelomonocytic leukemia
Authors: Faisal Muhammad , Stark Helge , Büsche Guntram , Schlue Jerome , Teiken Kristin , Kreipe Hans H. , Lehmann Ulrich , Bartels Stephan 
Published: January 2019

AbstractAtypical chronic myeloid leukemia (aCML) and chronic myelomonocytic leukemia (CMML) represent two histologically and clinically overlapping myelodysplastic/myeloproliferative neoplasms. Also the mutational landscapes of both entities show congruencies. We analyzed and compared an aCML cohort (n = 26) and a CMML cohort (n = 59) by next‐generation sequencing of 25 genes and by an nCounter approach for differential expression in 107 genes. Significant differences were found with regard to the mutation frequency of TET2, SETBP1, and CSF3R. Blast content of the bone marrow revealed an inverse correlation with the mutation status of SETBP1 in aCML and TET2 in CMML, respectively. By linear discriminant analysis, a mutation‐based machine learning algorithm was generated which placed 19/26 aCML cases (73%) and 54/59 (92%) CMML cases into the correct category. After multiple correction, differential mRNA expression could be detected between both cohorts in a subset of genes (FLT3, CSF3R, and SETBP1 showed the strongest correlation). However, due to high variances in the mRNA expression, the potential utility for the clinic is limited. We conclude that a medium‐sized NGS panel provides a valuable assistance for the correct classification of aCML and CMML.
How Histone Deacetylase Inhibitors Alter the Secondary Metabolites of Botryosphaeria mamane, an Endophytic Fungus Isolated from Bixa orellana
Authors: Triastuti Asih , Vansteelandt Marieke , Barakat Fatima , Trinel Manon , Jargeat Patricia , Fabre Nicolas , Amasifuen Guerra Carlos A. , Mejia Kember , Valentin Alexis , Haddad Mohamed 
Published: January 2019

AbstractFungi are talented organisms able to produce several natural products with a wide range of structural and pharmacological activities. The conventional fungal cultivation used in laboratories is too poor to mimic the natural habitats of fungi, and this can partially explain why most of the genes responsible for the production of metabolites are transcriptionally silenced. The use of Histone Deacetylase inhibitors (HDACis) to perturb fungal secondary biosynthetic machinery has proven to be an effective approach for discovering new fungal natural products. The present study relates the effects of suberoylanilide hydroxamic acid (SAHA) and sodium valproate (VS) on the metabolome of Botryosphaeria mamane, an endophytic fungus isolated from Bixa orellana L. UHPLC/HR‐MS analysis, integrated with four metabolomics tools: MS‐DIAL, MS‐FINDER, MetaboAnalyst and GNPS molecular networking, was established. This study highlighted that SAHA and VS changed metabolites in B. mamane, causing upregulation and downregulation of metabolites production. In addition, twelve compounds were detected in the extracts as metabolites structurally correlated to SAHA, indicating its important reactivity in the medium or its metabolism by the fungus. An addition of SAHA induced the production of eight metabolites while VS induced only two metabolites undetected in the control strain. This result illustrates the importance of adding HDACis to a fungal culture in order to induce metabolite production.
Mild Hydrogenation of α‐Pinene Catalyzed by Ru Nanoparticles Loaded on Boron‐doped Amphiphilic Core‐Shell Mesoporous Molecular Sieves
Authors: Yu Fengli , Xie Lihua , Wu Fangzhu , Yuan Bing , Xie Congxia , Yu Shitao , Liu Xien , Wang Lei , Wang Dan 
Published: January 2019

AbstractHighly dispersed and stable catalysts comprising Ru nanoparticles supported on boron‐doped amphiphilic core‐shell mesoporous molecular sieves (MMS−C@MMS−NH2/B/Ru) with alkyl‐modified hydrophobic silica core and NH2‐functionalized hydrophilic silica shell are successfully prepared for use in hydrogenation of α‐pinene for the first time. Dodecyl‐modified MMS−C12@MMS−NH2/B/Ru exhibits the best catalytic activity under mild hydrogenation conditions. The abundant −NH2 functional groups on the molecular sieve surface and their amphipathy allow the sieves to facilitate attachment of more Ru nanoparticles, and to simplify their dispersion in the water‐organic reaction medium. Moreover, B‐doped molecular sieves may adjust their acidity to meet the needs of α‐pinene hydrogenation. Under mild reaction conditions (25 °C, 1 MPa H2, and 1 h), α‐pinene can be completely converted with 99 % selectivity to cis‐pinane, because every nanocomposite is equivalent to a microreactor. The catalytic activity does not change much over 5 cycles, indicating that Ru nanoparticles are stably loaded on the molecular sieves.
Automatic navigation system for the mouse brain
Authors: Tappan Susan J. , Eastwood Brian S. , O'Connor Nathan , Wang Quanxin , Ng Lydia , Feng David , Hooks Bryan M. , Gerfen Charles R. , Hof Patrick R. , Schmitz Christoph , Glaser Jack R. 
Published: January 2019

AbstractIdentification and delineation of brain regions in histologic mouse brain sections is especially pivotal for many neurogenomics, transcriptomics, proteomics, and connectomics studies, yet this process is prone to observer error and bias. Here we present a novel brain navigation system, named NeuroInfo, whose general principle is similar to that of a global positioning system (GPS) in a car. NeuroInfo automatically navigates an investigator through the complex microscopic anatomy of histologic sections of mouse brains (thereafter: “experimental mouse brain sections”). This is achieved by automatically registering a digital image of an experimental mouse brain section with a three‐dimensional (3D) digital mouse brain atlas that is essentially based on the third version of the Allen Mouse Brain Common Coordinate Framework (CCF v3), retrieving graphical region delineations and annotations from the 3D digital mouse brain atlas, and superimposing this information onto the digital image of the experimental mouse brain section on a computer screen. By doing so, NeuroInfo helps in solving the long‐standing problem faced by researchers investigating experimental mouse brain sections under a light microscope—that of correctly identifying the distinct brain regions contained within the experimental mouse brain sections. Specifically, NeuroInfo provides an intuitive, readily‐available computer microscopy tool to enhance researchers' ability to correctly identify specific brain regions in experimental mouse brain sections. Extensive validation studies of NeuroInfo demonstrated that this novel technology performs remarkably well in accurately delineating regions that are large and/or located in the dorsal parts of mouse brains, independent on whether the sections were imaged with fluorescence or bright‐field microscopy. This novel navigation system provides a highly efficient way for registering a digital image of an experimental mouse brain section with the 3D digital mouse brain atlas in a minute and accurate delineation of the image in real‐time.
Correlations of Through‐Plane Cell Voltage Losses, Imbalance of Electrolytes, and Energy Storage Efficiency of a Vanadium Redox Flow Battery
Authors: Lim Hyebin , Yi Jung S. , Lee Doohwan 
Published: January 2019

AbstractCorrelations of the through‐plane voltage losses in the vanadium redox flow battery (VRFB), changes in the posolyte (positive electrolyte) and negolyte (negative electrolyte) conditions, energy storage capacity, and coulomb efficiency were investigated under an operando charge–discharge process. Collective and quantitative in situ studies with Ag/AgCl reference‐electrodes inserted into a VRFB single‐cell and the state of charge measuring external open‐circuit voltage half‐cells revealed that the overall performance of a VRFB is predominantly governed by (i) relatively high internal resistance of the anode half‐cell and (ii) conditions of the negolyte as the limiting electrolyte. The studies showed that the round‐trip coulomb efficiency, which is commonly used for VRFB performance analysis, has limitations in expressing the energy storage efficiency and capacity decay phenomena under a consecutive charge–discharge process. In situ and concurrent analysis of the internal cell overpotential and the electrolytes condition can provide quantitative information on the relative contributions of the coulombic round‐trip loss, concentration loss, vanadium crossover, and charge imbalance between the posolyte and negolyte for the overall efficiency and capacity decay phenomena. This operando analysis approach is highly effective for gaining an in‐depth and collective understanding of the complex phenomena taking place in the VRFB system.
High‐intensity breastfeeding improves insulin sensitivity during early post‐partum period in obese women with gestational diabetes
Authors: Yasuhi Ichiro , Yamashita Hiroshi , Maeda Kazuhisa , Nomiyama Makoto , Mizunoe Tomoya , Tada Katsuhiko , Yorozu Moe , Ogawa Masanobu , Kodama Takashi , Yamaguchi Ken , Okura Naofumi , Kawakami Kosuke , Maekawa Yuka , Hayashi Kimikazu 
Published: January 2019

AbstractAimTo investigate whether high‐intensity breastfeeding (HIB) reduces insulin resistance during early post‐partum period in women with gestational diabetes (GDM), independent of post‐partum weight change (PWC).Materials and methodsIn this multicentre prospective study, we included Japanese women with GDM who underwent a 75‐g oral glucose tolerance test (OGTT) during early post‐partum. We measured plasma insulin during OGTT to obtain a homeostasis model of assessment of insulin resistance (HOMA‐IR). We defined the condition in which infants were fed by breastfeeding alone or greater than or equal to 80% of the volume as HIB, and other statuses, including partial and nonbreastfeeding, as non‐HIB. We investigated the association between post‐partum HOMA‐IR and the breastfeeding status after adjusting for confounders including PWC.ResultsAmong 222 women with GDM who underwent the OGTT at 7.9 ± 2.3 weeks post‐partum with a PWC of −7.8 ± 3.4 kg, although the rate of abnormal glucose tolerance (prediabetes and diabetes) did not differ between the groups (33% vs 32%), the HOMA‐IR in the HIB women (n = 166) was significantly lower than that in the non‐HIB women (n = 56) (1.12 ± 0.85 vs 1.72 ± 1.43, P = 0.0002). The effect of the HIB was independently associated with lower HOMA‐IR after adjusting for confounders including PMC. However, the subgroup analysis according to their pre‐pregnancy obesity states showed that the effect was seen only in the obese subjects (BMI ≥ 25).ConclusionsIn obese Japanese women with GDM, HIB has a significant effect in reducing insulin resistance during early post‐partum, independent of the post‐partum weight loss.
Confident body, confident child: Evaluation of a universal parenting resource promoting healthy body image and eating patterns in early childhood—6‐ and 12‐month outcomes from a randomized controlled trial
Authors: Hart Laura M. , Damiano Stephanie R. , Li‐Wai‐Suen Connie S. N. , Paxton Susan J. 
Published: January 2019

AbstractObjectiveTo evaluate Confident Body, Confident Child (CBCC), a universal parenting resource designed to promote positive body image and healthy eating patterns in children aged 2–6 years, at 6‐ and 12‐months follow‐up.MethodA four‐arm randomized controlled trial with 345 parents was conducted. Group (A) received the CBCC resource pack + workshop, (B) received the CBCC resource pack only, (C) received a nutrition booklet and (D) received no interventions until all questionnaires were completed (i.e., waitlist control). Measures of parenting variables relevant to child body image and eating patterns, and parent‐report of child weight, were administered at baseline, 6‐weeks post‐intervention (results reported previously), and 6‐ and 12‐months follow‐up.ResultsMixed effects modeling comparing group averages over time revealed that significant group differences on measures of knowledge, parenting intentions and the parental feeding practice of weight restriction were still present at 12‐months follow‐up, though the remaining measures showed no significant differences between groups over time. The two CBCC groups reported more positive and less negative outcomes than the nutrition booklet active control.DiscussionThe CBCC program achieved sustained improvements in some parenting variables at 12‐months, suggesting its value as an effective parenting intervention. Changes to the intervention design, such as the addition of a follow‐up parent workshop, however, would likely increase its efficacy.
Portal hypertension in prolonged anorexia nervosa with laxative abuse: A case report of three patients
Authors: Koga Aiko , Toda Kenta , Tatsushima Keita , Matsuubayashi Sunao , Tamura Naho , Imamura Masatoshi , Kawai Keisuke 
Published: January 2019

AbstractObjectiveThere has been no report on portal hypertension related to anorexia nervosa (AN).MethodWe describe three cases of portal hypertension manifesting with collateral circulation represented by gastroesophageal varices in prolonged AN with laxative abuse and self‐vomiting. These women, in their 20s to 50s, were diagnosed as having AN binging and purging type (AN‐BP) that included self‐induced vomiting and abuse of irritating laxatives (more than 100 tablets daily).ResultsCase 1 showed prominent ascites and a gastro‐renal shunt on computed tomography scanning. Case 2 showed gastroesophageal varices on endoscopic examination. Case 3 showed gastroesophageal varices on computed tomography scanning and endoscopic examination. We performed liver biopsies in all patients and found only slight pericellular fibrosis. Our patients showed typical symptoms of portal hypertension, although liver cirrhosis was not present.DiscussionWe speculated that abnormal eating and purging behaviors were involved in the development of portal hypertension. We hypothesized that long‐term laxative abuse, dehydration, and abnormal eating behavior are involved in the development of portal hypertension, considering these were common features in our patients. Portal hypertension and gastroesophageal varices should be considered as one of the potentially existing complications in prolonged AN‐BP with self‐induced vomiting and abuse of irritating laxatives.
Characteristics of outpatients diagnosed with the selective/neophobic presentation of avoidant/restrictive food intake disorder
Authors: Zickgraf Hana F. , Murray Helen B. , Kratz Hilary E. , Franklin Martin E. 
Published: January 2019

AbstractObjectiveAlthough Avoidant/Restrictive Food Intake Disorder (ARFID) has existed since the publication of DSM‐5 in 2013, research on the descriptive psychopathology of treatment‐seeking patients with formal ARFID diagnoses is sparse, and limited to tertiary eating disorder‐focused treatment settings where most patients present with weight loss/malnutrition. In these settings, the selective/neophobic symptom presentation is rare compared to other primary eating restrictions. We aimed provide initial descriptive psychopathology of ARFID primary selective/neophobic symptom presentation in an outpatient setting, and to explore the prevalence of the core ARFID symptoms and clinical differences among patients meeting criteria based on weight/nutritional symptoms versus psychosocial impairment only.MethodWe reviewed the charts of 22 consecutive outpatients diagnosed with ARFID caused by selective/neophobic eating, and describe symptoms, impairment, illness trajectory, and demographic features. Patients who met ARFID criteria because of weight loss/nutritional problems were compared to those who met for psychosocial impairment only on demographic and clinical characteristics.ResultsPatients were predominantly male (81.8%) and school‐aged (4–11 years). 81.8% had no weight/nutritional symptoms documented by a medical provider. All met criteria for significant psychosocial impairment. There were few differences between patients who did versus did not meet weight loss/nutritional criteria for ARFID; they differed only in age and in the presence of appetite disturbances consistent with another proposed presentation of ARFID.DiscussionThese results provide novel data on the clinical characteristics of individuals who present with a primary presentation of selective/neophobic ARFID, including support for psychosocial impairment as sufficient for fulfilling ARFID criterion A.