bluuebunny/crossref_metadata_2025_split huggingface dataset:
Host phenotype is associated with reduced survival independent of tumour biology in patients with colorectal liver metastases
Authors: van Dijk David P.J. , Krill Matthew , Farshidfar Farshad , Li Ting , Rensen Sander S. , Olde Damink Steven W.M. , Dixon Elijah , Sutherland Francis R. , Ball Chad G. , Mazurak Vera C. , Baracos Vickie E. , Bathe Oliver F. 
Published: October 2018

AbstractBackgroundMost prognostic scoring systems for colorectal liver metastases (CRLMs) account for factors related to tumour biology. Little is known about the effects of the host phenotype to the tumour. Our objective was to delineate the relationship of systemic inflammation and body composition features [i.e. low skeletal muscle mass (sarcopenia) and low visceral adipose tissue (VAT)], two well‐described host phenotypes in cancer.MethodsClinical data and pre‐operative blood samples were collected from 99 patients who underwent resection of CRLM. Pre‐operative computed tomography scans were available for 97 patients; body composition was analysed at the L3 level, stratified for sex and age. Clinicopathological variables, serum C‐reactive protein (CRP), and various body composition variables were evaluated. Overall survival was evaluated as a function of these same variables in multivariate Cox regression analysis.ResultsSkeletal muscle was significantly correlated with VAT (r = 0.46, P < 0.001). Of patients with sarcopenia, 35 (65%) also had low VAT. C‐reactive protein was elevated (≥5 mg/mL) in 42 patients (43.3%). Elevated CRP was more common in patients with sarcopenia (57.4% vs. 33.3%, P = 0.029). The most significant prognostic factors were the coincidence of elevated CRP and adverse body composition features (sarcopenia and/or low VAT; hazard ratio 4.3, 95% confidence interval 1.5–13.0, P = 0.008), as well as Fong clinical prognostic score (hazard ratio 2.9, 95% confidence interval 1.5–5.5, P = 0.002).ConclusionsBody composition in patients with CRLM is not directly linked to the presence of systemic inflammation. However, when systemic inflammation coincides with sarcopenia and/or low VAT, prognosis is adversely affected, independent of the Fong clinical prognostic score.
Synthesis and Molecular Docking Study of Novel Hybrids of 1,3,4‐Oxadiazoles and Quinoxaline as a Potential Analgesic and Anti‐Inflammatory Agents
Authors: Dewangan Dhansay , Nakhate Kartik T. , Verma Vinay Sagar , Nagori Kushagra , Badwaik Hemant , Nair Nisha , Tripathi Dulal Krishna , Mishra Achal 
Published: October 2018

Novel hybrid molecules were synthesized through the amalgamation of quinoxaline residue with 1,3,4‐oxadiazole molecule. The purity of obtained 1,3,4‐oxadiazoles derivatives containing quinoxaline residue (total 11) was confirmed through thin‐layer chromatography, combustion analysis, and melting point, whereas their structures were confirmed by infrared spectroscopy, nuclear magnetic resonance, and mass spectroscopy. In animal studies, the derivatives 4‐(5‐(4‐(3‐methylquinoxalin‐2‐yl)phenyl)‐1,3,4‐oxadiazol‐2‐yl)benzenamine and 2‐(5‐(4‐(3‐methylquinoxalin‐2‐yl)phenyl)‐1,3,4‐oxadiazol‐2‐yl)benzenamine showed excellent analgesic and anti‐inflammatory activities, respectively, as compared with other derivatives. In order to rationalize the biological results of the derivatives, molecular docking studies were performed using Argus lab. The compounds exhibited good docking scores between −12.987 and −9.92 kcal/mol against cyclooxygenase‐II (5IKQ) protein fragment.
Repeated rectal application of a hyperosmolar lubricant is associated with microbiota shifts but does not affect PrEP drug concentrations: results from a randomized trial in men who have sex with men
Authors: Haaland Richard E , Fountain Jeffrey , Hu Yingtian , Holder Angela , Dinh Chuong , Hall LaShonda , Pescatore Nicole A , Heeke Sheila , Hart Clyde E , Xu Jiahui , Hu Yi‐Juan , Kelley Colleen F 
Published: October 2018

AbstractIntroductionOral pre‐exposure prophylaxis (PrEP) with tenofovir disoproxil fumarate (TDF) and emtricitabine (FTC) is highly effective in preventing HIV infection among men who have sex with men (MSM). The effects of consistent personal lubricant use in the rectum on tissue PrEP drug concentrations and the rectal microbiota are unknown. We investigated rectal PrEP drug concentrations and the microbiota in MSM before and after repeated rectal application of a hyperosmolar lubricant.MethodsWe randomized 60 HIV‐negative MSM to apply 4 mL of hyperosmolar rectal lubricant daily (n = 20), take daily oral TDF/FTC (n = 19), or both (n = 21) for seven days. Blood, rectal biopsies and rectal secretions were collected via rigid sigmoidoscopy before and on day 8 after product use. Tenofovir (TFV) and FTC as well as their intracellular metabolites tenofovir‐diphosphate (TFV‐DP), FTC‐triphosphate (FTC‐TP) were measured by HPLC‐mass spectrometry. Rectal mucosal microbiota was sequenced with 16S rRNA sequencing using Illumina MiSeq.ResultsSeven days of lubricant application was not associated with differences in PrEP drug concentrations in rectal tissue or secretions. Lubricant use was associated with a decrease in the relative abundance of the Bacteroides genus (p = 0.01) and a non‐significant increase in the Prevotella genus (p = 0.09) in the rectum. PrEP drug concentrations in rectal tissue and secretions were not associated with microbiota composition or diversity either before or after lubricant use.ConclusionsRepeated rectal application of a hyperosmolar lubricant does not affect mucosal PrEP drug concentrations but is associated with changes in the rectal microbiome.
The peripheral nerves of Lepidobatrachus tadpoles (Anura, Ceratophryidae)
Authors: Quinzio Silvia I. , Fabrezi Marissa 
Published: October 2018

AbstractThe peripheral nervous system of anuran larvae has traditionally been assumed to be largely invariant. Here, we describe the organization of cranial, spinal, and lateral line nerves at different larval stages of Lepidobatrachus spp. based on whole mounts. This is the first detailed description of cranial, spinal, and lateral lines innervation at premetamorphic stages of anuran larvae with notes on temporal variation. We distinguish three sources of morphological variation with respect to other anuran larvae: (a) the loss or reduction of some exclusively larval elements (i.e., the absence of the middle lateral line nerve); (b) spatial changes in the lateral line system (i.e., the supralabial arrangement of component of the anteroventral lateral line nerve); and (c) temporal changes in the disappearance of most of the lateral line system and in the premetamorphic repatterning of the spatial relationships of mandibularis ramus of the trigeminal (V) and hyomandibularis ramus of facial (VII). The innervation of limbs is achieved during late larval stages. Furthermore, comparisons among selected anurans reveal differences in tadpole brain morphology. The spatial and temporal variation found in the peripheral nerves of Lepidobatrachus larvae testifies to previously unappreciated variation in anuran larval morphology.
A unified scale for female reproductive stages in the Norway lobster (Nephrops norvegicus): Evidence from macroscopic and microscopic characterization
Authors: Becker Carola , Cunningham Eoghan M. , Dick Jaimie T. A. , Eagling Lawrence E. , Sigwart Julia D. 
Published: October 2018

AbstractKnowledge of the reproductive cycle in exploited species is important for a sustainable management of fisheries. Standardized scales to assess maturity stages are a fundamental tool to understand the demographic composition of exploited populations. Staging scales for female Norway lobster, Nephrops norvegicus, have been subject to a series of changes, and multiple inconsistent scales are in use in different fisheries regions. A unified, evidence‐based scale has not previously been established. We reviewed previous staging scales for the female ovary maturation and propose a revised scale based on the correlation between macroscopic and microscopic ovary characteristics. To provide better‐informed tools for future stock assessment, female stages were characterized through external observation on ovary color and size, and the progress of vitellogenesis. This study clarifies several biological phases that were conflated in previous scales. First, we demonstrate how to distinguish between immature ovaries in juvenile females versus the earliest ovary maturation stage in adults. Second, the new scale differentiates between “mottled” ovaries seen in two separate biological stages: the spent ovaries that undergo partial resorption in berried females, versus ovaries of females which failed to spawn and undergo full resorption. To ensure consistent application, colors are assessed relative to international standards (RAL/Pantone). This new, practical staging scheme clarifies the correlation between microscopic characteristics and macroscopically observable details in ovary maturation. Adoption of this unified staging scale will improve maturity analyses, help to identify stocks with potentially reduced reproductive capacity, and facilitate broad‐scale comparisons.
On the subjective experience and correlates of downtime at work: A mixed‐method examination
Authors: Lei Xue , Kaplan Seth A. , Dye Christy E. , Wong Carol M. 
Published: October 2018

SummaryIn the current paper, we studied downtime at work. Downtime represents a unique aspect of work time, as employees have low workload and more discretion about their activities but are still paid to be at work. Despite its prevalence and potentially significant implications for productivity and well‐being, the experience of downtime has not been a focus in the literature. To study this phenomenon, we first used a qualitative method involving 15 focus groups. Results from 95 employees showed that downtime is distinct from formal breaks and withdrawal behavior and is generally a negative subjective experience. Categories of antecedents of experience and downtime activities were classified. Next, to further explore the phenomenon, and based on Study 1 and relevant literature, we proposed several hypotheses and tested them using an experience sampling method. Results from 86 employees (across 5 workdays and 689 data points) indicated that the amount of downtime was negatively related to day level job satisfaction, job performance, and subjective health. The duration of relaxation activities during downtime mitigated the negative effects of downtime on fatigue. The effects of enjoyment level of the activities, job autonomy, work engagement, and boredom proneness were also examined. Implications, limitations, and future research are discussed.
Gender and career success: A typology and analysis of dual paradigms
Authors: Frear Katherine A. , Paustian‐Underdahl Samantha C. , Heggestad Eric D. , Walker Lisa Slattery 
Published: October 2018

SummaryDespite women's advancements in the workplace, gender inequality persists. We classify and test two frameworks used to explain gender differences in career success: unequal attributes and unequal effects. The unequal attributes framework suggests that gender is related to other attributes, which result in unequal career outcomes for men and women (i.e., a mediated effect). The unequal effects framework suggests that even when men and women share the same attribute or circumstances, they are rewarded differently, such that individual attributes have unequal effects on career outcomes for men and women (i.e., a moderated effect). We collected survey data from a gender‐balanced sample of 394 business school alumni. Using structural equation modeling to test the unequal attributes framework, we found that work hours, career orientation, having a nonemployed spouse, and working in a predominantly female job were unequal attributes that explained gender differences in career success. Using multigroup path analysis to examine unequal effects, we found that being agentic, married, having children at home, and working in a predominantly female workplace had unequal effects in relation to career success for men and women. We find support for both models across three categories of career success antecedents (i.e., personal, family, and job attributes).
Tenocyte cell density, migration, and extracellular matrix deposition with amniotic suspension allograft
Authors: Kimmerling Kelly A. , McQuilling John P. , Staples Miranda C. , Mowry Katie C. 
Published: October 2018

ABSTRACTAmniotic suspension allografts (ASA), derived from placental tissues, contain particulated amniotic membrane and amniotic fluid cells. Recently, ASA and other placental‐derived allografts have been used in orthopaedic applications, including tendinopathies and tendon injuries. The purpose of this study was to determine the potential effects of ASA on tenocyte cell density, migration, and responses to inflammatory stimuli. Tenocyte cell density was measured using AlamarBlue over multiple time points, while migration was determined using a Boyden chamber assay. Deposition of ECM markers were measured using BioColor kits. Gene expression and protein production of cytokines and growth factors following stimulus with pro‐inflammatory IL‐1β and TNF‐α was measured using qPCR and ELISAs. Conditioned media (CM) was made from ASA and used for all assays in this study. In vitro, ASA CM treatment significantly promoted tenocyte increases in cell density and migration compared to assay media controls. ASA CM also increased the deposition of extracellular matrix (ECM) proteins, including collagen, elastin, and sGAG. Following inflammatory stimulation and treatment with ASA CM, tenocytes downregulated IL‐8 gene expression, a pro‐inflammatory cytokine normally elevated during the inflammatory phase of tendon healing. Additionally, tenocytes treated with ASA CM had significantly lower protein levels of TGF‐β1 compared to controls. This study evaluated ASA and its effect on tenocytes; specifically, treatment with ASA resulted in increased cell density, more robust migration and matrix deposition, and some alteration of inflammatory targets. © 2018 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res 37:412–420, 2019.
Intra‐articular autologous uncultured adipose‐derived stromal cell transplantation inhibited the progression of cartilage degeneration
Authors: Kuroda Yuichi , Matsumoto Tomoyuki , Hayashi Shinya , Hashimoto Shingo , Takayama Koji , Kirizuki Shinsuke , Tsubosaka Masanori , Kamenaga Tomoyuki , Takashima Yoshinori , Matsushita Takehiko , Niikura Takahiro , Kuroda Ryosuke 
Published: October 2018

ABSTRACTThe role of uncultured adipose‐derived stromal cells for osteoarthritis treatment remains unclear despite sporadic reports supporting their use in clinical settings. This study aimed to evaluate the therapeutic effects of autologous uncultured adipose‐derived stromal cell transplantation in a rabbit osteoarthritis model. Uncultured adipose‐derived stromal cells isolated from rabbits were administered via intra‐articular injection into the knees after osteoarthritis onset. Animals were sacrificed at 8 and 12 weeks after osteoarthritis onset to compare the macroscopic, histological, and immunohistochemical characteristics between the uncultured adipose‐derived stromal cell and control groups. Co‐culture assay was also performed. The chondrocytes isolated from the model were co‐cultured with adipose‐derived stromal cells. The cell viability of chondrocytes and expression of chondrocyte‐specific genes in the co‐culture (uncultured adipose‐derived stromal cell) group were compared with the mono‐culture (control; chondrocytes only) group. In macroscopic and histological analyses, the uncultured adipose‐derived stromal cell group showed less damage to the cartilage surface than the control group at 8 and 12 weeks after osteoarthritis onset. In immunohistochemical and co‐culture assay, the uncultured adipose‐derived stromal cell group showed higher expression of collagen type II and SRY box‐9 and lower expression of matrix metalloproteinase‐13 than the control group. The cell viability of chondrocytes in the uncultured adipose‐derived stromal cell group was higher than that in the control group. Intra‐articular autologous uncultured adipose‐derived stromal cell transplantation inhibited the progression of cartilage degeneration in a rabbit osteoarthritis model by regulating chondrocyte viability and secreting chondrocyte‐protecting cytokines or growth factors, which promote anabolic factors and inhibit catabolic factors. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1376–1386, 2019.
MicroRNA‐146a negatively regulates the inflammatory response to Porphyromonas gingivalis in human periodontal ligament fibroblasts via TRAF6/p38 pathway
Authors: Tang Lu , Li Xudong , Bai Yuhao , Wang Pengcheng , Zhao Ying 
Published: October 2018

AbstractBackgroundHuman periodontal ligament fibroblasts (HPDLFs) represent the first line of defense against pathogens in the periodontal tissue. Porphyromonas gingivialis (P. gingivalis) has been known to be most strongly associated with periodontitis. MicroRNA (miR)‐146a is involved in the inflammatory regulation of periodontitis. However, the regulatory mechanism of miR‐146a on in P. gingivalis‐induced inflammation response in HPDLFs was still unclear. The aim of this study was to investigate whether miR‐146a plays a key role in P. gingvalis‐induced inflammation responses through regulation of TRAF6 in HPDLFs.MethodsMiR‐146a expression was measured by real‐time polymerase chain reaction (PCR) in HPDLFs stimulated with P. gingivalis and its lipopolysaccharide (LPS). IL‐1ß, IL‐6, and IL‐8 were determined by enzyme‐linked immunosorbent assay (ELISA) in the culture supernatants of HPDLFs after transfected with miR‐146a mimic or inhibitor. Meanwhile, the expression of TRAF6 was measured by real‐time PCR and Western blot. Then, we used luciferase reporter assay to detect whether miR‐146a binds to the 3′‐UTR of TRAF6. By using small interfering RNA (siRNA) of TRAF6, the phosphorylation of p38 mitogen‐activated protein kinase (MAPK) was measured by Western blot. Finally, after inhibition of TRAF6 and p38 in HPDLFs, we analyzed the expression of miR‐146a upon P. gingivalis challenge.ResultsP. gingivalis and its LPS significantly induced miR‐146a expression in HPDLFs. Overexpression of miR‐146a significantly suppressed the IL‐1ß, IL‐6 and IL‐8 secretion, TRAF6 expression, and p38 phosphorylation. In contrast, the levels of these indexes significantly increased by inhibition of miR‐146a. Furthermore, MiR‐146a directly binds to the 3′‐UTR of TRAF6 in P. gingivalis‐induced HPDLFs, but not in P. gingivalis LPS stimulation. Suppression of TRAF6 could inhibit the phosphorylation of p38. Finally, inhibition of TRAF6 and p38 significantly abolished P. gingivalis‐induced miR‐146a upregulation in HPDLFs.ConclusionsMiR‐146a contribute to negative regulation of P. gingivalis‐induced proinflammatory cytokines secretion in HPDLFs though TRAF6/p38 MAPK pathway. Maintaining miR‐146a homeostasis plays a key role in controlling inflammatory response in periodontal tissues.
Relationship between rheumatoid arthritis and periodontal disease in Korean adults: Data from the Sixth Korea National Health and Nutrition Examination Survey, 2013 to 2015
Authors: Jung Eun Seo , Choi Yoon Young , Lee Kyeong Hee 
Published: October 2018

AbstractBackgroundThis study aims to investigate the relationship between rheumatoid arthritis and periodontal disease using data from the Sixth Korea National Health and Nutrition Examination Survey (KNHANES, 2013 to 2015).MethodsOf 22,948 KNHANES participants, 14,264 who were aged ≥19 years and responded to questions pertaining to periodontal disease and rheumatoid arthritis were analyzed. Periodontal status was measured using the Community Periodontal Index. The authors used a complex sampling analysis by applying an individual sampling weighting to maintain the rolling survey sampling method. To determine the prevalence of rheumatoid arthritis and periodontal disease, a Chi‐squared test was performed. Logistic regression analysis was performed after controlling for selected variables to determine relevance.ResultsThe prevalence of rheumatoid arthritis in patients with periodontal disease was 1.6%, which was higher than in individuals without periodontal disease (1.5%). The prevalence of periodontal disease in individuals with rheumatoid arthritis was 28.4%, which was higher than in subjects without rheumatoid arthritis (27.9%). The risk for periodontal disease was 1.64 times higher in individuals with rheumatoid arthritis than in those without rheumatoid arthritis. Regardless of age or sex, the risk for periodontal disease was 1.97 times higher in the presence of rheumatoid arthritis. However, other logistic regression analysis models, when adjusted for socioeconomic‐, health‐, and oral health‐related factors, did not yield statistically significant findings.ConclusionsThe study results suggest a possible relationship between rheumatoid arthritis and periodontal disease. Further large cohort studies investigating causal relationships between rheumatoid arthritis and periodontal disease are needed.
Appraisal of the potential of the growth of e‐retailer based on impact of perceived value on trust for online purchases
Authors: Mohiuddin Babu Mujahid , Dey Bidit Lal 
Published: October 2018

AbstractCustomer satisfaction for online purchase and its subsequent influence on customer trust is a pivotal in the proliferation of online businesses that in turn can foster global and national economic growth. The significance of understanding perceived value in the context of online shopping is as important as is in the context of traditional shopping. Quantitative data were collected from customers, who have online shopping experience. The analysis, carried out using structural equation modeling, suggests that customers' perceived acquisition value has a significant impact over their satisfaction which affects their trust with regard to an online retailer.
Metabolite profiling of l‐isocorypalmine in rat urine, plasma, and feces after oral administration using high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry
Authors: Li Yiran , Zhang Teng , Huai Jiaxin , Cheng Congcong , Xie Linlin , Wang Siqi , Dai Ronghua 
Published: October 2018

Abstractl‐Isocorypalmine, an active alkaloid compound isolated from Rhizoma Corydalis yanhusuo, has been reported to possess biological activity for treating cocaine use disorder. A high‐performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry method was established for identification of the metabolites of l‐isocorypalmine in urine, plasma and feces samples of rats after a single intragastric gavage of l‐isocorypalmine at a dose of 15 mg/kg. As a result, a total of 21 metabolites (six phase І metabolites and fifteen phase II metabolites) were detected and tentatively identified by mass spectrometry and fragment ions from tandem mass spectrometry spectra. All metabolites were present in the urine samples, nine metabolites were found in the plasma samples and three metabolites were found in the feces samples. Results indicated that metabolic pathways of l‐isocorypalmine included oxidation, dehydrogenation, demethylation, sulfate conjugation, and glucuronide conjugation. In addition, glucuronidation was the major metabolic reaction. Results of this investigation could provide significant experimental basis for efficacy, safety and action mechanism of l‐isocorypalmine, which will be advantageous to new drug development for treating cocaine addiction.