bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crocins: The Active Constituents of Crocus Sativus L. Stigmas, Exert Significant Cytotoxicity on Tumor Cells In Vitro
Authors: Hatziagapiou Kyriaki , Kakouri Eleni , Lambrou George I. , Koniari Eleni , Kanakis Charalabos , Nikola Olti A. , Theodorakidou Margarita , Bethanis Konstantinos , Tarantilis Petros A. 
Published: October 2018

Background::Tumors of the childhood are considered to be grave and devastating pathologies, with high mortality rates. Current therapeutic options like cytotoxic drugs and radiotherapy target both healthy and malignant cells, thus resulting in long-term neurological and intellectual sequelae and endocrinological disorders.Objectives::In this study, we focused on the anticancer potency of crocins, the main constituents of Crocus sativus L, stigmas. Crocins were first extracted using organic solvents from the dried stigmas and then were identified using the HPLC analysis.Materials and Methods::TE-671 cells were treated with the extract of crocins using a range of concentrations between 0.25-mg/ mL and 16 mg/mL. Viability of the cells was measured at 24h, 48h, 72h and 96h. In addition, we have examined the expression levels of the p53 gene using Real-Time Reverse Transcription PCR.Results::Results showed that crocins exerted significant cytotoxic and anti-proliferative effects in a concentration and time - dependent-manner on TE-671 cells. Furthermore, p53 manifested similar expression pattern as the anti-proliferative effect of crocin.Conclusion::Our data demonstrate that crocins could be a novel promising agent for the improvement of tumor treatment.
Design and Computational Modeling of Spiral Microfluidic Channel for Sorting and Separating the Biomolecules
Authors: Praveenkumar S. , Sridhar P. Arjunan , Lingaraja D. , Dinesh Ram G. 
Published: October 2018

Background:
Microfluidic technologies are a very challenging area today in the field of
biomolecule analysis. This has become feasible with the today’s advanced technologies by designing
and fabricating the microfluidic channel.

Materials and Methods:
Initially, microfluidic channels are used to separate large molecules,
where the molecular dimension of the fluidic filter is greater than the gap size. In this work, separation
of biomolecules (like RBC, WBC and platelets) that are smaller than the microfluidic filter
gap size is demonstrated.

Results and Conclusion:
Due to the curvilinear nature of the spiral, there exists two vortices called
dean vortices within the channel and this is influenced by dean flow, centrifugal flow and tubular
pinch effect. While flowing a small aliquot of blood in the channel, due to these three effects,
molecules attain equilibrium position at one point. The position of equilibrium will be different for
different sized biomolecules and this varies with different input velocities.

Conclusion:
The obtained computational modeling results show how the equilibrium positions influence
the separation efficiency of biomolecules in passive based microfluidic filter. Compared
with the traditional random nanoporous materials such as gel or polymer monolith, spiral based
microfluidic channels can be made precisely to have a pre-determined loop count and Dean Flow
number (De).
Self Recurrent Neural Network Based Target Tracking in Wireless Sensor Network using State Observer
Authors: Jondhale Satish R. , Deshpande Rajkumar S. 
Published: October 2018

Background & Objective:Mobile target tracking based on data from wireless sensor networks (WSN) is a hot topic that has been investigated both from a theoretical and practical point of view in the literature. Tracking the position and velocity of a target moving in WSN (especially in the context of uncertain noisy measurement channel) is a very challenging task. To deal with the uncertainty in system dynamics as well as uncertainty in target states, an Observer Based Self Recurrent Neural Network (OBSRNN) is proposed in this paper.Methods:The proposed algorithm employs a state observer based tracking control strategy and thereby allows for accurate estimation of mobile target moving along a predefined route in WSN. The Self Recurrent Neural Network (SRNN) framework is used to approximate the uncertainty in the system dynamics, while a full-order state observer is used to estimate the unknown state vector.Conclusion:The simulation analysis is performed to evaluate the efficacy of the proposed work.
Governanc?a Corporativa na estrutura conceitual do Relato Integrado: Divulgac?ões das Instituições Bancárias Brasileiras
Authors: Tavares Lucia Maria , Henrique Valesca Dias , Manoel Jorge Roberto , Santos Fernando De Almeida , Fernandes dos Santos Neusa Maria Bastos 
Published: October 2018

Este artigo busca identificar, analisar e salientar a transparência das instituições bancárias brasileiras, por meio das informações contidas em seus relatos integrados, de acordo com as diretrizes estabelecidas pelo International Integrated Reporting Council (IIRC) e pela Global Reporting Initiative (GRI). Trata-se de um relatório completo e complexo, que deve ser elaborado com clareza e transparência, tanto para seus sócios e investidores, quanto para a sociedade. Nele devem conter informações como capital humano, financeiro, intelectual, de relacionamento, natural e manufaturado. As instituições bancárias brasileiras que aderiram ao RI, demonstram aqui que contribuem não só com seus clientes, mas com todos os stakeholders, de forma sustentável, transparente e ética. A metodologia utilizada foi a te?cnica denominada ana?lise de conteu?do, com uma abordagem de natureza qualitativa sobre os relatos integrados das instituições bancárias brasileiras, restrito aos aspectos relacionados a? governanc?a corporativa, a fim de verificar a forma como as empresas aderiram ao modelo proposto pela estrutura conceitual, sugerida pelo IIRC. A coleta de dados, em conformidade com a te?cnica de ana?lise de conteu?do, segue os procedimentos de pesquisa documental, na qual os dados utilizados foram a estrutura conceitual para relato integrado (IIRC, 2013) dos relato?rios corporativos das 25 instituições bancárias, listadas pela BMFBOVESPA. Constatou-se que, dos bancos avaliados, apenas 5 instituições publicam o relato integrado em 2016, mas apenas 3 dessas 5 instituições cumpriram todos os requisitos do relato integrado.
Mini-implant supported canine retraction with micro-osteoperforation: A split-mouth randomized clinical trial
Authors: Sivarajan Saritha , Doss Jennifer Geraldine , Papageorgiou Spyridon N. , Cobourne Martyn T. , Wey Mang Chek 
Published: October 2018

ABSTRACT

Objectives:
To investigate, using a split-mouth randomized clinical design, the effect of micro-osteoperforation (MOP) on mini-implant supported canine retraction using fixed appliances.

Materials and Methods:
Thirty subjects (seven males and 23 females) with a mean age of 22.2 (3.72) years were randomized into three canine retraction groups: Group 1 (MOP 4-weekly maxilla/8-weekly mandible; n = 10); Group 2 (MOP 8-weekly maxilla/12-weekly mandible; n = 10) and Group 3 (MOP 12-weekly maxilla/4-weekly mandible; n = 10) measured at 4-week intervals over 16 weeks. Subjects also completed pain (5-point Likert scale) and pain impact (Visual Analogue Scale) questionnaires. The primary outcome was the amount of canine retraction over 16 weeks at MOP (experimental) and non-MOP (control) sites.

Results:
Mean overall canine retraction was 4.16 (1.62) mm with MOP and 3.06 (1.64) mm without. After adjusting for differences between jaws, all MOP groups exhibited significantly higher canine distalization than the control group: 0.89 mm more (95% confidence interval [CI] = 0.19 to 1.59 mm; P = .01) in the MOP-4 group, 1.08 mm more (95% CI = 0.49 to 1.68 mm; P = .001) in the MOP-8 group and 1.33 mm more (95% CI = 0.55 to 2.10 mm; P = .002) in the MOP-12 group. All subjects reported pain associated with MOP with 60% classifying it as moderate and 15% severe. The main impact of this reported pain was related to chewing and speech.

Conclusions:
MOP can increase overall mini-implant supported canine retraction over a 16-week period of observation but this difference is unlikely to be clinically significant.
Análisis de tecnologías para la transformación de material aglomerado de madera con fines de reciclaje
Authors: Reyes Echeverría Aura Karina , Bautista Rojas Luis Eduardo , Quintero Cárdenas Luis Carlos 
Published: October 2018

Los Tableros de Partículas y Tableros de Fibra de madera están compuestos de agua y aglomerantes como la urea-formaldehido que hacen su proceso de degradación más lento. En la transformación de tablero se generan residuos que son utilizados como combustible o depositados en vertederos municipales, lo cual es perjudicial para el medio ambiente debido a las sustancias químicas nocivas utilizadas en el proceso de adhesión y conformación del tablero. El objetivo de este trabajo es realizar una búsqueda de tecnologías para la transformación de los residuos de material aglomerado de madera, con fines de reúso o reciclaje. Se realizó una identificación de palabras clave, se elaboró una ecuación de búsqueda, se ejecutó en dos bases de datos científicas y en webgrafía. Los resultados fueron analizados para orientar la aplicación de los procesos de transformación. Como resultados se analizaron 31 artículos de investigación, 6 casos de uso, se concluyen 7 procesos químicos y 5 procesos físicos.
ANÁLISIS DE FORMANTES VOCÁLICOS NORMALIZADOS Y NO-NORMALIZADOS PARA EL ESPAÑOL COLOMBIANO
Authors: Navia Heriberto J. Rangel , Rodriguez Daniela Andrea Chinchilla , Calderon Jennifer Lisbeth Espinosa 
Published: October 2018

INTRODUCCIÓN: Definir valores de referencia para diagnóstico del habla a través de los formantes vocálicos normalizados y no-normalizados del español colombiano. MÉTODOS: Estudio transversal con inclusión población aleatoria por muestreo estratificado. 111 participantes 45 hombres, 66 mujeres. Recolección de datos con frase vehicular para vocales sostenidas. Analisis No-Normalizado usando mediana y media por genero y edad. Análisis Normalizado a través de Labov y Nearey. RESULTADOS: Los valores de referencia encontrados son: (1) No-Normalizados: /a/ F1 867,03 Hz, F2 1435,85 Hz, /e/ F1 482,69 Hz, F2 2152,30 HZ; /i/ F1 330,48 Hz, F2 2400,11 Hz; /o/; F1 488,62 Hz, F2 984,22Hz; /u/ F1 368,93 Hz, F2 815,88 Hz; (2) Normalizción Labov: /a/ F1 1106,8 Hz, F2 1831,1 Hz, /e/ F1 616,5 Hz, F2 2755,4 Hz; /i/ F1 422 Hz, F2 3072 Hz; /o/; F1 623,7 Hz, F2 1255,8 Hz; /u/ F1 471 Hz, F2 1047,7 Hz; (3) Normalización Nearey /a/ F1 846 Hz, F2 1347 Hz, /e/ F1 467 Hz, F2 1954 Hz; /i/ F1 312 Hz, F2 2190 Hz; /o/; F1 463 Hz, F2 940 Hz; /u/ F1 350 Hz, F2 759 Hz. ANÁLISIS Y DISCUSIÓN: El método de normalización que da cuenta de la conformación acústica y articulatoria del español colombiano es el de Nearey con inclusión del tercer formante para el cálculo del centroide S. CONCLUSIONES: La inclusión del tercer formante durante la normalización de Nearey permite ajustar los valores del Espacio Vocálico a la configuración acústica y articulatoria del español.
Effect of radiographic technique on assessment of liver size in Beagles
Authors: Cha Ahyoung , Park Seungjo , Kim Choelhyun , Yoon Sooa , Lee Dahae , Kim Dongeun , Chang Dong Woo , Choi Jihye 
Published: October 2018

Abstract
OBJECTIVE To investigate the effects of respiratory phase, body position, beam center location, and gastric distention on radiographic assessment of liver size in dogs.
ANIMALS 12 Beagles.
PROCEDURES Liver length and the ratio of liver length to T11 length were determined on lateral radiographic views obtained with various techniques. Images were acquired at maximal expiration or maximal inspiration, with dogs in right or left recumbency, with the beam centered on the caudal border of the scapula or the 13th rib, and after food was withheld or with gastric distention. Effects on organs adjacent to the liver were assessed with CT. Changes of the thoracic cavity during the respiratory cycle were investigated with fluoroscopy.
RESULTS Liver length was significantly greater on radiographs obtained at maximal expiration than at maximal inspiration, but there was no increase in the ratio of liver length to T11 length. Body position, beam center location, and gastric distention did not significantly affect liver size. For CT, location of the spleen and stomach and location or size of the liver did not change markedly between right and left recumbency. Fluoroscopy revealed that thoracic width was less at maximal expiration than maximal inspiration.
CONCLUSIONS AND CLINICAL RELEVANCE Liver length was greater at maximal expiration than at maximal inspiration because of a smaller thoracic width. Body position, beam center location, and gastric distention did not affect liver length. The ratio of liver length to T11 length was not significantly affected by any of the factors investigated.
Effects of a nutrient-enriched water with and without poultry flavoring on water intake, urine specific gravity, and urine output in healthy domestic cats fed a dry kibble diet
Authors: Zanghi Brian M. , Wils-Plotz Emma , DeGeer Staci , Gardner Cari L. 
Published: October 2018

Abstract
OBJECTIVE To investigate water intake and urine measures in healthy cats provided free-choice access to a nutrient-enriched water with (NWP) or without (NW) added poultry flavoring offered at 3 different volumes in addition to tap water (TW).
ANIMALS 36 domestic shorthair cats.
PROCEDURES Control group cats (n = 4) received dry food with TW ad libitum throughout the study. Cats of the NW and NWP groups (n = 16/group) received the same food with TW only (period 1; 7 days) followed by TW and the assigned treatment ad libitum at 1X, 1.5X, and 2X the volume of TW consumed in period 1 during periods 2 (17 days), 3 (10 days), and 4 (10 days), respectively. Liquid consumption, food intake, and total water intake (from all sources) were measured; urine collected over 48 hours in each period was measured, and urine specific gravity (USG) was determined. Data were analyzed with mixed-effects models.
RESULTS TW and food calorie intake were similar among groups in period 1; TW consumption by control cats did not differ during the study. Liquid consumed by drinking increased 18%, 57%, and 96% for the NWP group in periods 2, 3, and 4, respectively, with increases of 25% and 44% for the NW group in periods 3 and 4, respectively, compared with period 1 values for the same groups. Increased urine output and decreased USG were significantly associated with period and treatment.
CONCLUSIONS AND CLINICAL RELEVANCE Increasing the volumes of NW or NWP offered to healthy cats led to increased free liquid consumption and was associated with greater urine output and dilution as measured by USG. Studies are warranted to determine whether these treatments provide health benefits for cats in need of greater water consumption.
Risk of environmental exposure to small coccidia from wild canid feces in rural Ohio
Authors: Moreno-Torres Karla I. , Sinnott Devinn M. , Wolfe Barbara A. , Marsh Antoinette E. , Saville William J. A. , Moritz Mark , Garabed Rebecca B. 
Published: October 2018

Abstract
OBJECTIVE To determine the extent of environmental exposure to heteroxenous coccidia from wild canid feces in southeastern Ohio.
SAMPLE 285 presumed wild canid fecal samples collected across an ecological system in southeastern Ohio.
PROCEDURES Morphological classification and molecular analysis were used to determine the canid genus for collected fecal samples. Microscopic and molecular analysis were used to detect coccidian oocysts and DNA. Several variables were analyzed for associations with coccidian DNA detection or prevalence.
RESULTS Coccidian DNA was detected in 51 of 285 (17.9%) fecal samples. Of those positive samples, 1% (95% confidence interval, 0.4% to 3%) had positive results for Hammondia heydorni and none had positive results for Neospora caninum, for an estimated environmental N caninum prevalence of 0% (95% confidence interval, 0% to 7%)/1-km2 hexagonal area evaluated. Morphological classification revealed that 78.9% (225/285) of fecal samples were from coyotes and 17.2% (49/285) were from foxes. No difference in proportions of coccidian DNA-positive fecal samples was identified among canid species. Environmental temperature and fecal freshness were associated with coccidian DNA detection. Land use type, relative canid density, and cattle density were not associated with the prevalence of coccidian DNA-positive samples.
CONCLUSIONS AND CLINICAL RELEVANCE The low prevalence of coccidia shed in wild canid feces in this study, including the estimated 0% environmental prevalence of N caninum, suggested that the role of the oocyst environmental phase in coccidia transmission to ruminants is likely minor in rural southeastern Ohio.
Effects of furosemide administration to water-deprived inland bearded dragons (Pogona vitticeps)
Authors: Parkinson Lily A. , Mans Christoph 
Published: October 2018

Abstract
OBJECTIVE To evaluate the diuretic effects and associated changes in hematologic and plasma biochemical values following SC furosemide administration to water-deprived inland bearded dragons (Pogona vitticeps).
ANIMALS 9 bearded dragons.
PROCEDURES In a crossover study design, furosemide (5 or 10 mg/kg) was administered SC every 12 hours for 4 doses or no treatment (control treatment) was provided for the same period. Food and water were withheld. Body weight was recorded before (baseline) and 12 hours after treatment sessions ended and then after 5 minutes of soaking in a water bath. Blood samples were collected at baseline and 12 hours after treatment sessions ended for various measurements.
RESULTS Compared with control values, a significant decrease from baseline in body weight was detected after furosemide treatment at 5 and 10 mg/kg (mean ± SD percentage decrease, 5.5 ± 3.2% and 5.2 ± 4.1%, respectively). Soaking resulted in a significant increase in body weight after the 5- and 10-mg/kg furosemide treatments (mean ± SD percentage increase, 2.9 ± 1.8% and 5.6 ± 2.5%, respectively), compared with change in body weight after the control treatment (0.7 ± 0.7%). Plasma total solids and total protein concentrations increased significantly with both furosemide treatments, and PCV increased significantly with the 10 mg/kg treatment only. No significant or relevant differences were identified in plasma osmolarity or uric acid or electrolyte concentrations.
CONCLUSIONS AND CLINICAL RELEVANCE Furosemide as administered resulted in hemoconcentration and weight loss in bearded dragons, most likely owing to its diuretic effects. With additional research, furosemide could be considered for treatment of congestive heart failure and other conditions requiring diuresis in bearded dragons.
Circulating miR-210 and miR-1246 as potential biomarkers for differentiating hepatocellular carcinoma from metastatic tumors in the liver
Authors: Ahmed Emad K. , Fahmy Shaimaa A. , Effat Heba , Wahab Abdel Hady Abdel 
Published: October 2018

SummaryBackgroundTo date few reports have pointed out the role of circulating miRNAs in discriminating metastatic liver tumors from primary hepatocellular (HCC) tumors. Such discrimination will have significant therapeutic and prognostic implications. The purpose of this study was to evaluate the potential value of a panel of HCC-related circulating miRNAs (miR-142, miR-182, miR-200a, mir-210, miR-211, miR-302b, miR-324, miR-338, miR-340 and miR-1246) as noninvasive biomarkers for discriminating primary HCC from metastatic tumors in the liver.MethodsThe expression level of the selected miRNAs was quantified by quantitative real time PCR in 33 patients with HCC, 22 patients with metastatic tumors in the liver, and 30 healthy volunteers as control. Mann-Whitney U test was used to evaluate the difference in miRNAs expression between primary and metastatic liver tumors and to study the associations between their relative expression levels and the clinicopathological factors. Receiver operating characteristic curve was used to evaluate the diagnostic value of the individual miRNAs.ResultsStatistical analyses revealed a differential expression in the level of serum miR-210 and miR-1246 between the two groups of patients. The sensitivity and specificity of miR-210, for differentiating HCC from metastatic malignancies in the liver were found to be 73.7% and 64.28%, respectively. Whilst, of miR-1246 were 72.2% and 67.8%, respectively. In addition, the differential expression of the two miRNAs was also found to be associated with clinicopathological parameters in the two studied groups.ConclusionsSerum miR-210 and miR-1246 have some diagnostic value for discriminating patients with metastatic tumors to patients with primary HCC
T cell subsets’ distribution in elite karate athletes as a response to physical effort
Authors: Kostrzewa-Nowak Dorota , Buryta Rafał , Nowak Robert 
Published: October 2018

SummaryBackgroundDuring karate fight muscles work at a very high intensity, and their contractions are extremely strong. The movement pattern contains a great number of feints, dodges, frequent changes in movements’ tempo and direction, hits and kicks, all of which is highly stressful for athlete’s organism, including the immune system.MethodsT lymphocyte subsets’ distribution and selected cytokines in peripheral blood of three elite karate athletes aged 30 years old (range 21–31 years) with minimum 15 years of training experience were analysed in two experiments: at the beginning of the preparatory phase (a progressive test until exhaustion; an analysis of immune system’s selected parameters and cardiorespiratory fitness measures, including VO2max, VE, AT, MVV, MET, Rf), and during the start-up period (Karate Championships; an analysis of selected parameters of the immune system).ResultsMaximal effort caused an increase in total lymphocyte percentage (p<0.05). A decrease in Th cells in recovery (p<0.05 compared to post-exercise), and an increase in Th naïve cells in recovery (p<0.05) were observed. A significant increase in CD8+central memory cells (p<0.05) was found only after the progressive test, and no changes in both central and effector memory subsets of CD4+cells during the first experiment. An increase (p<0.05) in Treg and Th1 and a decrease (p<0.05) in Th2 cells’ distribution during recovery time were found. Additionally, changes (p<0.05) in TNF-α, IL-6, IL-8, IL-10 and IL-12p70 were observed.ConclusionPost-effort disorder in immune balance activated compensation pathways involving CD4+cells. Treg and Th1 cells seem to be subsets of key importance involved in the anabolic effect of physical effort, at least among karate athletes.