bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proenkephalin A 119-159 (penKid) – a novel biomarker for acute kidney injury in sepsis: An observational study.
Authors: Rosenqvist Mari , Bronton Kevin , Hartmann Oliver , Bergmann Andreas , Struck Joachim , Melander Olle 
Published: August 2019

Abstract
Background Sepsis is a leading cause of death worldwide and a major challenge for physicians to predict and manage. Proenkephalin A 119-159 (penKid) is a reliable surrogate marker for the more unstable endogenous opioid peptide enkephalin, which has previously been shown to predict both acute and chronic kidney disease. The aim of this prospective observational study was to assess penKid as a predictor of acute kidney injury (AKI), multi-organ failure and mortality in sepsis among unselected sepsis patients presenting to the emergency department (ED).Method We measured penKid in 588 adult patients with sepsis (≥2SIRS criteria + suspected infection) upon admission to the ED at Skåne University Hospital, Malmö, Sweden. and used logistic regression to relate income levels of penKid to outcomes. Logistic regression models were used to derive odds ratios per 1-SD increment of log-transformed penKid to AKI, multi-organ failure, 28-day mortality as well as to progression of renal SOFA subscore (rSOFA) 0 or 1 to higher rSOFA.Results In age and sex adjusted models, penKid predicted AKI development within 48 hours and 7 days, however, these associations were attenuated after additional adjustment for estimated creatinine-based glomerular filtration rate (eGFR). In models adjusted for age, sex and eGFR, penKid significantly predicted progression from income rSOFA=0 and from rSOFA≤1 to higher rSOFA scores as well as multi-organ failure and mortality. In contrast, eGFR did not predict 28-day mortality.Conclusion PenKid is an effective predictor of renal injury, severe multi-organ failure and mortality in unselected sepsis patients presenting to the emergency department.
Investigation of novel biomarkers of drug-induced kidney injury in renal transplant recipients undergoing graft biopsy
Authors: Michon Arthur , Durrbach Antoine , Gautier Jean-Charles , Benain Xavier , Lunven Catherine , Jagerschmidt Alexandre , Aubert Catherine , Poetz Oliver , Joos Thomas , Gury Thierry , Becquemont Laurent 
Published: August 2019

Abstract
Urinary and blood kidney biomarkers (BM) remain insufficient to detect early kidney injury. Our aim was to compare new kidney BM with histopathological data in kidney allograft recipients.

Blood and urine samples of consecutive adult patients were collected just prior graft biopsy. All kidney samples were classified according to Banff 2007.
The diagnostic performance (area under ROC curve, AUROC) of 16 new BM was compared to those of urinary proteins, blood urea nitrogen, eGFR and serum creatinine to identify histopathological groups.

223 patients were analyzed. Versus slightly modified renal parenchyma (SMRP), microalbuminuria and urinary proteins had the highest diagnostic performance toward glomerular injury. Urinary neutrophil gelatinase associated lipocalin (NGAL) had the best performance values for acute tubular necrosis (ATN) versus SMRP (AUROC 0.93). Others BM reached slightly lower AUROC reaching 0.89. When comparing ATN to acute rejection several new urinary BM (NGAL, cystatin C, and MCP1) and classical BM (eGFR, serum creatinine) reached similar AUROC values ranging from 0.80 to 0.85. Values of urinary NGAL were 10-fold higher in ATN compared to acute rejection (p = 0.0004).

New BM did not outperform classical BM during renal transplantation. Urinary NGAL might be helpful to discriminate ATN and acute rejection.
The role of temporal changes in endometrial blood flow in natural and hormone replacement cycles with vitrified-warmed embryo transfer
Authors: Ota Kuniaki , Takahashi Toshifumi , Shiraishi Satoru , Mizunuma Hideki 
Published: August 2019

Abstract
Background Uterine blood flow promotes endometrial development and subsequent implantation of fertilized eggs. We examined the temporal changes in uterine blood flow during natural and hormone replacement (HRT) cycles and clarified the relationship between uterine blood flow and assisted reproductive technology outcomes for vitrified-warmed embryo transfer (ET). Methods This was a retrospective cohort study. A total of 60 patients with vitrified-warmed ET were assigned to two groups according to the endometrial preparation: natural cycle (28 patients) or HRT cycle (32 patients). The uterine endometrial blood flow was evaluated using measurements of the radial artery resistance index (RA-RI) during the early follicular phase, the days of the human chorionic gonadotropin (hCG) trigger during a natural cycle or the start of progesterone administration during the HRT cycle, and the day of ET. We statistically and longitudinally measured and evaluated the RA-RI values of all individual patients. Results During natural cycles, the RA-RI on the day of ET was significantly higher than that during the early follicular phase. During HRT cycles, the RA-RI on the day of ET was also significantly increased compared to that during the early follicular phase. We validated the efficacy of the RA-RI values for predicting the possibility of pregnancy with vitrified-warmed ET. During natural cycles, the area under the receiver-operating characteristic curves (AUCs) for the early follicular phase, the day of hCG trigger, and the day of ET were 0.75 (95% confidence interval [CI], 0.57–0.93), 0.69 (95% CI, 0.49–0.88), and 0.60 (95% CI, 0.36–0.84), respectively. During HRT cycles, AUCs for the early follicular phase, the day of starting progesterone administration, and the day of ET were 0.60 (95% CI, 0.40–0.81), 0.60 (95% CI, 0.39–0.87), and 0.58 (95% CI, 0.37–0.79), respectively. Conclusions The uterine RA-RI increased at approximately the time of the implantation window compared to that of the early follicular phase during both natural and HRT cycles with vitrified-warmed ET. Our findings suggest that RA-RI during the early follicular phase might be effective and useful for deciding whether to choose the natural or HRT cycle for vitrified-warmed ET.
Health professionals’ experiences of providing care for women survivors of sexual violence in psychiatric inpatient units.
Authors: O'Dwyer Carol , Tarzia Laura , Fernbacher Sabin , Hegarty Kelsey 
Published: August 2019

Abstract
Background Survivors of sexual violence commonly access mental health services. Psychiatric inpatient units in Australia are predominately mixed gender and may further retraumatise these women. Sexual violence is under-recognised by mental health professionals and there is a lack of adequate policy or direction for mental health service services. To date, only a small amount of research has focused on health professionals’ experiences of providing trauma-informed care to women in psychiatric settings, with most studies focused on specific practices or interventions. Qualitative data is particularly lacking on this topic. This is a critical gap in the knowledge given that health professionals are key to detecting and addressing victimisation. The aim of this study was to gain an in-depth understanding of healthcare professionals’ experiences and perceptions in providing care to women who are survivors of sexual violence in psychiatric inpatient units.Methods This qualitative study utilised semi-structured interviews with 40 health professionals recruited from four psychiatric inpatient units within a large Australian public mental health organisation. Data were examined using thematic analysis.Results Three main themes were developed to describe participants’ experiences of the care provided to women; 1) Dismissing and denying; 2) Acknowledging but unprepared; 3) Empathising but despairing. Discussion Gender, professional training, adherence to the biomedical model, and level of experience influenced health professionals’ experiences.Conclusions Health professionals in this study held varying attitudes towards female consumers and responses to sexual violence. Our findings suggest the need to address individual staff perception and promote trauma-informed and gender-sensitive care across all disciplines, genders, and levels of experience.
People living in residential aged care need to BE outside not just SEE outside: associations between quality of life and outdoor access: a cross-sectional study
Authors: Dyer Suzanne Marie , Liu Enwu , Gnanamanickam Emmanuel , Harrison Stephanie Louise , Milte Rachel , Crotty Maria 
Published: August 2019

Abstract
Background

The value of providing access to outdoor areas for people living in residential aged care, including those living with dementia, in terms of mood, behaviour and well-being is increasingly acknowledged. This study examines associations between provision of independent access to outdoor areas and frequency of residents going outdoors with the quality of life (QoL) of nursing home residents and compares use of outdoor areas between alternative models of residential aged care.

Methods

A cross-sectional study was conducted including 541 participants from 17 residential aged care homes in four states in Australia, mean age 85 years, 84% with cognitive impairment. Associations between having independent access to outdoors and the frequency of going outdoors and QoL (EQ-5D-5L) were examined using multi-level models. The odds of going outdoors in a small-scale home-like model of care compared to standard Australian models of care were examined.

Results

After adjustment for potential confounders (including comorbidities and facility level variables), living in an aged care home with independent access to the outdoors was not significantly associated with QoL (β=-0.01, 95% Confidence Interval (CI) -0.09 to 0.07, P=0.80). However, going outdoors daily (β=0.13 95%CI 0.06 to 0.21), but not multiple times a week (β=0.03, 95%CI -0.03 to 0.09), was associated with a better QoL. Residents living in a home-like model of care had greater odds of going outdoors daily (odds ratio 15.1, 95%CI 6.3 to 36.2).

Conclusions

Going outdoors frequently is associated with higher QoL for residents of aged care homes and residents are more likely to get outside daily if they live in a small-scale home-like model of care. However, provision of independent access to outdoor areas alone may be insufficient to achieve these benefits. Increased availability of models of residential aged care with staffing structures, training and design which increases support for residents to venture outdoors frequently is needed to maximise resident quality of life.
Nurse-Patient Interaction and Self-Transcendence: Assets for a Meaningful Life in Nursing Home Residents?
Authors: Haugan Gørill , Kuven Britt Moene , Eide Wenche Mjanger , Tåsen Siw Eriksen , Rinnan Eva , Wu Vivien Xi , Drageset Jorunn , André Beate 
Published: August 2019

Abstract
Background.
Today, we face a shift to an older population worldwide and its consequences; a noteworthy part of older adults will need 24-hours nursing home care at the end of life. Finding new and alternative approaches to increase wellbeing among nursing home residents is highly warranted. Knowledge about nurse-patient-interaction, self-transcendence and meaning-in-life seems vital in order to guide clinical practice in how to best and efficiently boost wellbeing among older adults in nursing homes.

Methods
In a cross-sectional design, 188 (92% response rate) out of 204 long-term NH patients representing 27 NHs responded to the nurse-patient interaction, self-transcendence, and the purpose-and-meaning-in-life scales. Inclusion criteria were: (1) municipality authority’s decision of long-term NH care; (2) residential time three months or longer; (3) informed consent competency recognized by responsible doctor and nurse; and (4) capable of being interviewed. The hypothesized relations (five hypotheses) between the latent constructs were tested by means of structural equation modelling (SEM) using Stata 15.1.

Results
The SEM-model yielded a good fit (χ2=146.824, p=0.021, df=114, χ2 /df=1.29 RMSEA=0.040, p-close 0.811, CFI=0.97, TLI=0.96, and SRMR=0.063), showing significant relationships between the constructs of nurse-patient interaction, inter- and intra-personal self-transcendence and meaning-in-life. Nurse-patient interaction significantly relates with both inter- and intra-personal self-transcendence and meaning in NH patients. Self-transcendence revealed a fundamental influence on perceived meaning, while nurse-patient interaction demonstrated a significant influence on meaning, mediated by self-transcendence

Conclusion
According to the rapidly growing number of people over 65 in the world, and the growing segment of people 80-100, the present results are significant in their suggestions that nurse-patient-interaction is a crucial resource in relation to nursing home residents’ wellbeing. Knowledge of how nurse-patient-interaction, self-transcendence and meaning relate to each other among older adults in NHs is important for researchers, nurses, caregivers, nursing educators, and clinicians. Health professionals in nursing homes should learn how to competently use the nurse-patient interaction as a health promoting asset for self-transcendence, meaning and thus well-being. Nursing home nurses should be given more time for interacting with the residents, continuity and mutuality in nurse-patient relationships should be prioritized and facilitated.
A Meta-analysis for Major Complications Between Traditional Pacemaker and Leadless Pacemaker
Authors: Cui Diyu , Chen Xiaorui , Wang Lin , Liao Yimeng , He Huan , Chen Yunqing 
Published: August 2019

Abstract
Objectives To compare the major complications between leadless pacemaker (LP) and traditional pacemakers(TP).Background The TP shows some advantages in avoiding pocket- and lead-related complications over the TP and is increasingly used in clinical practice. However, the clinical effect of LP remains controversial.Methods PUBMED、EMBASE、The COCHRANE LIBRARY、CNKI and WANGFANG databases were searched from July 2013 to August 2018. Data concerning the study’s design, patients’ characteristics and outcomes were extracted. The primary end-point is the major complications. The second end-points are elevated pacing threshold, cardiac perforation/effusion, device dislodgement and vascular events.Results A total of 6 studies fulfilled the inclusion criteria. Only 4 of which can provide the data of major complications. The main complications of LP were statistically significantly decreased compared with that of TP(OR 0.41,95%CI:0.29-0.56,P<0.00001,I2=42%).We extracted the data of elevated pacing threshold, cardiac perforation/effusion, device dislodgement and vascular events in 4 other of these 6 studies. There was no significant difference in elevated pacing threshold (OR 0.95, 95%CI:0.24-3.70,P=0.94, I2=31%), cardiac perforation/effusion(OR 1.78,95%CI:0.33-9.58, P=0.50,I2=87%), vascular events (OR1.58, 95%CI:0.45-5.53, P=0.47,I2=47%) and device dislodgement(OR 0.22,95%CI:0.01-5.69,P=0.36,I2=81%) between LP and TP.Conclusion Compared with TP, LP showed a significantly decreased risk in major complications. This indicates that LP has a good prospect to be applicated in clinical practice.
NRF2 metagene signature is a novel prognostic biomarker in colorectal cancer
Authors: O'Cathail Séan Micheal , Wu Chieh-Hsi , Lewis Annabelle , Holmes Chris , Hawkins Maria A , Maughan Tim 
Published: August 2019

Abstract
Background NRF2 overexpression confers poor prognosis in some cancers but its prognostic role in colorectal cancer (CRC) is unknown. As a transcription factor, we hypothesise a signature of NRF2 regulated genes could act as a prognostic biomarker in CRC and reveal novel biological insights.Methods Using known NRF2 regulated genes, differentially expressed in CRC, we defined a signature of NRF2 pathway expression using principal component analysis and Cox proportional hazard models and tested it in four independent mRNA datasets, profiled on three different mRNA platforms.Results 36 genes comprised the final NRF2 signature. 1360 patients were included in the validation. High NRF2 was associated with worse disease free survival (DFS) and/or overall survival (OS) in all datasets: (GSE14333 HR=1.55, 95% C.I 1.2–2.004, p = 0.0008; GSE39582 HR=1.24, 95% C.I 1.086–1.416, p = 0.001; GSE87211 HR=1.431, 95% C.I 1.06–1.93, p = 0.056; MRC FOCUS trial HR=1.14, 95% C.I 1.04–1.26, p = 0.008). In multivariate analyses, NRF2 remained significant when adjusted for stage and adjuvant chemotherapy in stage I-III disease, and BRAF V600E mutation and sidedness in stage IV disease. NRF2 expression was particularly enriched in Consensus Molecular Subtype (CMS) 4.Conclusion For the first time, NRF2 is shown to be a consistent, robust prognostic biomarker across all stages of colorectal cancer with additional clinical value to current known prognostic biomarkers. High NRF2 signalling in CMS 4 further refines the molecular taxonomy of CRC, a new biological insight, suggesting avenues of further study.
Antibody-guided Chromatin Tagmentation (ACT-seq)
Authors: Carter Benjamin , Zhao Keji , Ku Wai Lim , Kang Jee Youn , Tang Qingsong 
Published: August 2019

Abstract

ACT-seq is a streamlined method for mapping genome-wide distributions of histone tail modifications, histone variants, and chromatin-binding proteins in a small number of or single cells. ACT-seq utilizes a fusion of Tn5 transposase to Protein A that is targeted to chromatin by a specific antibody, allowing chromatin fragmentation and sequence tag insertion specifically at genomic sites presenting the relevant antigen. The Tn5 transposase enables the use of an index multiplexing strategy (iACT-seq), which enables construction of thousands of single-cell libraries in one day by a single researcher without the need for drop-based fluidics or visual sorting. The protocol described here is intended for use with bulk-cell samples. The single-cell iACT-seq protocol is separate.
Modeling the impact of agricultural crops on the spatial and seasonal variability of water balance components in the Lake Tana basin, Ethiopia
Authors: Tigabu Tibebe B. , Wagner Paul D. , Hörmann Georg , Fohrer Nicola 
Published: August 2019

AbstractThe Lake Tana basin hosts more than three million people and it is well known for its water resource potential by the Ethiopian government. The major economic activity in the region is agriculture, but the effect of agricultural crops on water resources is poorly understood. Understanding the crop water interaction is important to design proper water management plans. Therefore, the primary objective of this research is to investigate the effect of different agricultural crops on the spatial and seasonal variability of water balance components of Gilgelabay, Gumara, and Ribb catchment areas of Lake Tana basin, Ethiopia. To this end, the hydrologic model SWAT (Soil and Water Assessment Tool) was used to simulate the water fluxes between 1980 and 2014. The water balance components, which were mapped for each hydrologic response unit, indicated the spatial variations of water fluxes in the study. Cereal crops like teff and millet had significant effect in enhancing groundwater recharge, whereas leguminous crops like peas had significant impact in increasing runoff generation. Moreover, the model outputs showed that the total streamflow is dominated by baseflow and about 13%, 9%, and 7% of the annual rainfall goes to the deep aquifer system of Gilgelabay, Gumara, and Ribb catchment areas, respectively.
Performance of ANN, SVM and MLH techniques for land use/cover change detection at Sultan Marshes wetland, Turkey
Authors: Kesikoglu M. Hayri , Atasever U. Haluk , Dadaser-Celik Filiz , Ozkan Coskun 
Published: August 2019

Abstract
Wetlands are among the most productive ecosystems that provide services ranging from flood control to climate change mitigation. Wetlands are also critical habitats for the survival of numerous plant and animal species. In this study, we used satellite remote sensing techniques for classification and change detection at an internationally important wetland (Ramsar Site) in Turkey. Sultan Marshes is located at the center of semi-arid Develi closed basin. The wetlands have undergone significant changes since the 1980s due to changes in water flow regimes, but changes in recent years have not been sufficiently explored yet. In this study, we focused on the changes from 2005 to 2012. Two multispectral ASTER images with spatial resolution of 15 m, acquired on June 11, 2005 and May 20, 2012, were used in the analyses. After geometric correction, the images were classified into four information classes, namely water, marsh, agriculture, and steppe. The applicability of three classification methods (i.e. maximum likelihood (MLH), multi-layer perceptron type artificial neural networks (ANN) and support vector machines (SVM)) was assessed. The differences in classification accuracies were evaluated by the McNemar's test. The changes in the Sultan Marshes were determined by the post classification comparison method using the most accurate classified images. The results showed that the highest overall accuracy in image classifications was achieved with the SVM method. It was observed that marshes and steppe areas decreased while water and agricultural areas expanded from 2005 to 2012. These changes could be the results of water transfers to the marshes from neighboring watershed.
Respuestas del maíz que indican que el paclobutrazol es una sustancia que induce mayor actividad genética
Authors: Velázquez- Alcaraz Teresa De Jesús , Díaz-Valdés Tomás , Ayala-Tafoya Felipe , Yáñez Juárez Moisés Gilberto , Partida- Ruvalcaba Leopoldo , Medina López Raymundo , López-Orona Carlos A. 
Published: August 2019

El objetivo de esta investigación fue determinar el efecto que produce el Paclobutrazol (PBZ) en la actividad genética de los cultivares de maíz Puma 1076, 30P49 y DK2020 a través de la evaluación de las variables de respuesta verdor, altura de planta, diámetro del tallo, longitud y ancho de hoja, peso y volumen de 1000 granos, contenido de proteína y rendimiento de grano por hectárea. El diseño experimental fue bloques completos al azar con cinco repeticiones en dos experimentos realizados durante los ciclos agrícolas 2008-2009 y 2009-2010; en el primer ciclo se utilizaron parcelas de cuatro surcos de 40 m de largo, donde se aplicaron 200 kg de N ha-1; mientras que en el segundo experimento la longitud de las parcelas fue de 5,0 m y se fertilizó con 250 kg de N ha-1. Las dosis de PBZ fueron 150, 300 y 450 mg L-1 de agua al exponerse la cuarta, sexta y octava hoja verdadera, respectivamente. Los resultados indicaron que el PBZ indujo mayor actividad genética, ya que la síntesis de proteínas y de otros componentes de células, tejidos y órganos es un proceso que depende de la transcripción de mensajes del ADN en el ARNm, de la síntesis de aminoácidos, del transporte de aminoácidos por el ARNt y de la unión de aminoácidos por la enzima Peptidil Transferasa, lo que conlleva a la traducción de mensajes en proteínas estructurales y activas (enzimas), para formar más células y sustancias que constituyen a éstas y, en consecuencia, ocasionar más rendimiento de grano por hectárea.