bluuebunny/crossref_metadata_2025_split huggingface dataset:
Andrographolide attenuates imbalance of gastric vascular homeostasis induced by ethanol through glycolysis pathway
Authors: Yao Huan , Wu Ziqiang , Xu Yiming , Xu Huan , Lou Guanhua , Jiang Qing , Fan Weichuan , Liu Weiming , Zheng Chuan , Gao Yongxiang , Wang Yong 
Published: March 2019

AbstractDifferent kinds of factors contribute to gastric ulcer development which characterized by damaging gastric mucosal layer. However, gastric vascular homeostasis is not well defined and whether andrographolide has a protective function is largely unknown. The goal of this study is to investigate the potential function roles and underlying mechanism by which andrographolide regulates gastric vascular homeostasis in vivo and in vitro. Gastric ulcer animal model induced on andrographolide pretreated C57/BL6 mouse by ethanol intragastric administration. Hematoxylin and Eosin Stain, Masson’s trichrome stain and Immunohistochemistry stain performed to observe gastric vascular homeostasis, which associated hemorrhage, extracellular matrix deposition and macrophage infiltration. The activity of vascular endothelial cells were associated with the proliferation and migration, which were detected using cell counting, MTS, and wound scratch healing assay. The underlying endothelial glycolytic mechanism investigated in vivo and in vitro. Andrographolide pretreatment dramatically attenuates ethanol intragastric administration induced imbalance of gastric vascular homeostasis which characterized by severe hemorrhage, increase extracellular matrix deposition and augment macrophage infiltration. Andrographolide treatment conspicuous inhibits HUVEC-C activity characterized by suppressing proliferation and migration of endothelial cells. Mechanically, andrographolide treatment significant suppresses the expression of glycolytic genes, especially decrease PFKFB3 expression. The treatment with PFKFB3 inhibitor, 3-PO, exacerbates the inhibitory function of andrographolide on vascular endothelial cell proliferation and migration. Those data Suggests that andrographolide contributes to maintain gastric vascular homeostasis, at least partially, by inhibiting PFKFB3 mediated glycolysis pathway. Andrographolide plays a crucial role in maintaining gastric vascular homeostasis during gastric ulcer development through regulating vascular endothelial cell glycolytic pathway.
Targeting hormone refractory prostate cancer by in vivo selected DNA libraries in an orthotopic xenograft mouse model
Authors: Civit Laia , Theodorou Ioanna , Frey Franziska , Weber Holger , Lingnau Andreas , Gröber Carsten , Blank Michael , Dambrune Chloé , Stunden James , Beyer Marc , Schultze Joachim , Latz Eicke , Ducongé Frédéric , Kubbutat Michael H. G. , Mayer Günter 
Published: March 2019

AbstractThe targeting of specific tissue is a major challenge for the effective use of therapeutics and agents mediating this targeting are strongly demanded. We report here on an in vivo selection technology that enables the de novo identification of pegylated DNA aptamers pursuing tissue sites harbouring a hormone refractory prostate tumour. To this end, two libraries, one of which bearing an 11 kDa polyethylene glycol (PEG) modification, were used in an orthotopic xenograft prostate tumour mouse model for the selection process. Next-generation sequencing revealed an in vivo enriched pegylated but not a naïve DNA aptamer recognising prostate cancer tissue implanted either subcutaneous or orthotopically in mice. This aptamer represents a valuable and cost-effective tool for the development of targeted therapies for prostate cancer. The described selection strategy and its analysis is not limited to prostate cancer but will be adaptable to various tissues, tumours, and metastases. This opens the path towards DNA aptamers being experimentally and clinically engaged as molecules for developing targeted therapy strategies.
The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity
Authors: Monteiro João M. , Covas Gonçalo , Rausch Daniela , Filipe Sérgio R. , Schneider Tanja , Sahl Hans-Georg , Pinho Mariana G. 
Published: March 2019

AbstractBacterial cells are surrounded by cell wall, whose main component is peptidoglycan (PG), a macromolecule that withstands the internal turgor of the cell. PG composition can vary considerably between species. The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family. FemX adds the first glycine of the cross bridge, while FemA and FemB add the second and the third, and the fourth and the fifth glycines, respectively. Of these, FemX was reported to be essential. To investigate the essentiality of FemAB, we constructed a conditional S. aureus mutant of the femAB operon. Depletion of femAB was lethal, with cells appearing as pseudomulticellular forms that eventually lyse due to extensive membrane rupture. This deleterious effect was mitigated by drastically increasing the osmolarity of the medium, indicating that pentaglycine crosslinks are required for S. aureus cells to withstand internal turgor. Despite the absence of canonical membrane targeting domains, FemA has been shown to localise at the membrane. To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding. Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation. Our data indicates that, contrarily to what was previously thought, S. aureus cells do not survive in the absence of a pentaglycine cross bridge.
IL-27 promotes NK cell effector functions via Maf-Nrf2 pathway during influenza infection
Authors: Kumar Pawan , Rajasekaran Kamalakannan , Nanbakhsh Arash , Gorski Jack , Thakar Monica S. , Malarkannan Subramaniam 
Published: March 2019

AbstractInfluenza virus targets epithelial cells in the upper respiratory tract. Natural Killer (NK) cell-mediated early innate defense responses to influenza infection include the killing of infected epithelial cells and generation of anti-viral cytokines including interferon gamma (IFN-γ). To date, it is unclear how the underlying cytokine milieu during infection regulates NK cell effector functions. Our data show during influenza infection myeloid cell-derived IL-27 regulates the early-phase effector functions of NK cells in the bronchioalveolar and lung tissue. Lack of IL-27R (Il27ra−/−) or IL-27 (Ebi3−/−) resulted in impaired NK cell effector functions including the generation of anti-viral IFN-γ responses. We identify CD27+CD11b+NK cells as the primary subset that expresses IL-27R, which predominantly produces IFN-γ within the upper respiratory tract of the infected mice. IL-27 alone was incapable of altering the effector functions of NK cells. However, IL-27 sensitizes NK cells to augment bothin vitroandin vivoresponses mediated via the NKG2D receptor. This ‘priming’ function of IL-27 is mediated partly via transcriptional pathways regulated by Mafs and Nrf2 transcriptionally regulating TFAM and CPT1. Our data for the first time establishes a novel role for IL-27 in regulating early-phase effector functions of NK cells during influenza infection.
Effective and Reliable Framework for Lung Nodules Detection from CT Scan Images
Authors: Khan Sajid Ali , Hussain Shariq , Yang Shunkun , Iqbal Khalid 
Published: March 2019

AbstractLung cancer is considered more serious among other prevailing cancer types. One of the reasons for it is that it is usually not diagnosed until it has spread and by that time it becomes very difficult to treat. Early detection of lung cancer can significantly increase the chances of survival of a cancer patient. An effective nodule detection system can play a key role in early detection of lung cancer thus increasing the chances of successful treatment. In this research work, we have proposed a novel classification framework for nodule classification. The framework consists of multiple phases that include image contrast enhancement, segmentation, optimal feature extraction, followed by employment of these features for training and testing of Support Vector Machine. We have empirically tested the efficacy of our technique by utilizing the well-known Lung Image Consortium Database (LIDC) dataset. The empirical results suggest that the technique is highly effective for reducing the false positive rates. We were able to receive an impressive sensitivity rate of 97.45%.
Majorana flat band edge modes of topological gapless phase in 2D Kitaev square lattice
Authors: Zhang K. L. , Wang P. , Song Z. 
Published: March 2019

AbstractWe study a Kitaev model on a square lattice, which describes topologically trivial superconductor when gap opens, while supports topological gapless phase when gap closes. The degeneracy points are characterized by two vortices in momentum space, with opposite winding numbers. We show rigorously that the topological gapless phase always hosts a partial Majorana flat band edge modes in a ribbon geometry, although such a single band model has zero Chern number as a topologically trivial superconductor. The flat band disappears when the gapless phase becomes topologically trivial, associating with the mergence of two vortices. Numerical simulation indicates that the flat band is robust against the disorder.
Controlling Chaotic Resonance using External Feedback Signals in Neural Systems
Authors: Nobukawa Sou , Shibata Natsusaku 
Published: March 2019

AbstractStochastic resonance is a phenomenon in which the signal response of a non-linear system is enhanced by appropriate external noise. Likewise, a similar phenomenon can be caused by deterministic chaos; this is called chaotic resonance. Devices that employ stochastic resonance have been proposed for the purpose of enhancing tactile sensitivity. However, no applications of chaotic resonance have been reported so far, even though chaotic resonance exhibits a higher sensitivity than stochastic resonance. This contrast in applications could be attributed to the fact that chaotic resonance is induced by adjusting internal parameters. In many cases, especially in biological systems, these parameters are difficult to adjust. In this study, by applying our proposed reduced region of orbit method to a neural system consisting of excitatory and inhibitory neurons, we induce chaotic resonance with signal frequency dependency against weak input signals. Furthermore, the external noise exhibits effects for both diminishing and enhancing signal responses in chaotic resonance. The outcome of this study might facilitate the development of devices utilising the mechanism of chaotic resonance.
Gene Saturation: An Approach to Assess Exploration Stage of Gene Interaction Networks
Authors: Yin Ziqiao , Guo Binghui , Mi Zhilong , Li Jiahui , Zheng Zhiming 
Published: March 2019

AbstractThe gene interaction network is one of the most important biological networks and has been studied by many researchers. The gene interaction network provides information about whether the genes in the network can cause or heal diseases. As gene-gene interaction relations are constantly explored, gene interaction networks are evolving. To describe how much a gene has been studied, an approach based on a logistic model for each gene called gene saturation has been proposed, which in most cases, satisfies non-decreasing, correlation and robustness principles. The average saturation of a group of genes can be used to assess the network constructed by these genes. Saturation reflects the distance between known gene interaction networks and the real gene interaction network in a cell. Furthermore, the saturation values of 546 disease gene networks that belong to 15 categories of diseases have been calculated. The disease gene networks’ saturation for cancer is significantly higher than that of all other diseases, which means that the disease gene networks’ structure for cancer has been more deeply studied than other disease. Gene saturation provides guidance for selecting an experimental subject gene, which may have a large number of unknown interactions.
Stochastic modelling reveals mechanisms of metabolic heterogeneity
Authors: Tonn Mona K. , Thomas Philipp , Barahona Mauricio , Oyarzún Diego A. 
Published: March 2019

AbstractPhenotypic variation is a hallmark of cellular physiology. Metabolic heterogeneity, in particular, underpins single-cell phenomena such as microbial drug tolerance and growth variability. Much research has focussed on transcriptomic and proteomic heterogeneity, yet it remains unclear if such variation permeates to the metabolic state of a cell. Here we propose a stochastic model to show that complex forms of metabolic heterogeneity emerge from fluctuations in enzyme expression and catalysis. The analysis predicts clonal populations to split into two or more metabolically distinct subpopulations. We reveal mechanisms not seen in deterministic models, in which enzymes with unimodal expression distributions lead to metabolites with a bimodal or multimodal distribution across the population. Based on published data, the results suggest that metabolite heterogeneity may be more pervasive than previously thought. Our work casts light on links between gene expression and metabolism, and provides a theory to probe the sources of metabolite heterogeneity.
Negative capacitance from the inductance of ferroelectric switching
Authors: Cheng Po-Hsien , Yin Yu-Tung , Tsai I-Na , Lu Chen-Hsuan , Li Lain-Jong , Pan Samuel C. , Shieh Jay , Shiojiri Makoto , Chen Miin-Jang 
Published: March 2019

AbstractNegative capacitance (NC) has been proposed to realize sub-Boltzmann steep-slope transistors in recent years. We provide experimental evidences and theoretical view for ferroelectric NC and inductance induced by polarization switching, based on an as-deposited nanoscale ferroelectric zirconium oxide (ZrO2) layer (nano-f-ZrO2). The experimental results are demonstrated in nano-f-ZrO2, including resistor–inductor–capacitor oscillations, positive reactance in Nyquist impedance plot, enhancement of capacitance, and sub-60 mV/dec subthreshold swing of nanoscale transistors. The theoretical analysis shows that ferroelectric polarization switching yields an effective electromotive force which is similar in behavior to Lenz’s law, leading to inductive and NC responses. Nano-beam electron diffraction reveals ferroelectric multi-domains in nano-f-ZrO2. Under small-signal operation, the switching of net polarization variation in ferroelectric multi-domains contributes to the ferroelectric inductance and NC. Nano-f-ZrO2 provides a pronounced inductance compared to conventional inductors, which would have impacts in a variety of applications including transistors, filters, oscillators, and radio-frequency-integrated circuits.
USP39 regulates the cell cycle, survival, and growth of human leukemia cells
Authors: Liu Chunxia , Yao Xiaojian , Li Ming , Xi Yaming , Zhao Li 
Published: March 2019

Abstract
Ubiquitin-specific peptidase 39 (USP39) is one member of the cysteine proteases of the USP family, which represents the largest group of DeUbiquitinases with more than 50 members in humans. The roles of USP39 in human cancer have been widely investigated. However, the roles of USP39 in human leukemia and the underlying mechanism remain unknown. Here we reported the function of USP39 in human leukemia. We observed that the expression of USP39 was up-regulated in human leukemia cells and the high expression of USP39 was correlated with poor survival of the patients with leukemia. Lentivirus-mediated knockdown of USP39 repressed the proliferation and colony formation of human leukemia cell lines HL-60 and Jurkat cells. Mechanism study showed that USP39 knockdown induced the arrest of cell cycle and apoptosis of leukemia cells. In addition, our microarray and bioinformatic analysis demonstrated that USP39 regulated diverse cellular signaling pathways that were involved in tumor biology, and several pivotal genes (IRF1, Caspase 8, and SP1) have been validated by quantitative real-time polymerase chain reaction. Knockdown or IRF1 partially restored the proliferation rate of leukemia cells with USP39 knockdown. Taken together, our findings implicate that USP39 promotes the development of human leukemia by regulating cell cycle, survival, and proliferation of the cells.
Individual Versus Small Group Treatment of Morphological Errors for Children With Developmental Language Disorder
Authors: Eidsvåg Sunniva S. , Plante Elena , Oglivie Trianna , Privette Chelsea , Mailend Marja-Liisa 
Published: March 2019

Purpose

This study examines the effects of enhanced conversational recast for treating morphological errors in preschoolers with developmental language disorder. The study assesses the effectiveness of this treatment in an individual or group (
n
= 2) setting and the possible benefits of exposing a child to his or her partner's treatment target in addition to his or her own.

Method

Twenty children were assigned to either an individual (
n
= 10) or group (
n
= 10, 2 per group) condition. Each child received treatment for 1 morpheme (the target morpheme) for approximately 5 weeks. Children in the group condition had a different target from their treatment partner. Pretreatment and end treatment probes were used to compare correct usage of the target morpheme and a control morpheme. For children in the group condition, the correct usage of their treatment partner's target morpheme was also examined.

Results
Significant treatment effects occurred for both treatment conditions only for morphemes treated directly (target morpheme). There was no statistically significant difference between the treatment conditions at the end of treatment or at follow-up. Children receiving group treatment did not demonstrate significant gains in producing their partner's target despite hearing the target modeled during treatment.

Conclusions
This study provides the evidence base for enhanced conversational recast treatment in a small group setting, a treatment used frequently in school settings. Results indicate the importance of either attention to the recast or expressive practice (or both) to produce gains with this treatment.

Supplemental Material

https://doi.org/10.23641/asha.7859975
New bow-shock source with bipolar morphology in the vicinity of Sgr A*
Authors: Peißker F. , Zajaček M. , Eckart A. , Sabha N. B. , Shahzamanian B. , Parsa M. 
Published: March 2019

Context. We find an extended source in the direct vicinity of Sgr A* with an approximate projected mean distance of 425 ± 26 mas. Its sky-projected elongated shape can be described by an averaged spatial extension ofx = 110 ± 20 mas andy = 180 ± 20 mas. With this, the observed object points in the analyzed SINFONI data sets between 2006 and 2016 directly toward the supermassive black hole. We discuss different possible scenarios that could explain the detected blueshifted line emission source.Aims. Here we present a detailed and extensive analysis of the adaptive optics corrected SINFONI data between 2006 and 2016 with a spatial pixel scale of 0.″025 and a corresponding field of view of 0.″8 × 0.″8 per single data cube with the focus on the newly discovered source. We spectroscopically identify the source, which we name X8, in the blueshifted Brγline maps. Additionally, an upper limit for the continuum magnitude can be derived from the close-by S-star S41.Methods. We applied the standard reduction procedure with the SINFONI/EsoRex pipeline for the analysis. We applied pre- and post-data correction in order to establish various calibration procedures. For the sharpened images, we used the Lucy–Richardson algorithm with a low iteration number. For the high-pass filtered images, we used the smooth-subtracting process in order to increase the signal-to-noise ratio.Results. We are able to detect the elongated line emission source in quantified data sets between 2006 and 2016. We find a lower limit for the infrared continuum magnitude ofKs ≳ 17.0 ± 0.1. The alignment of X8 toward Sgr A* can be detected in data sets that fulfill a sufficient number of observations with a defined quality level. A more detailed analysis of the results shows indications of a bipolar outflow source that might be associated with either a young stellar object, or with a post-AGB star or young planetary nebula.Conclusions. The near-infrared excess source X8 close to S24, S25, and S41 can be detected between 2006 and 2016. In addition to an apparent bow-shock morphology, the source shows clear signatures of a bipolar outflow that is consistent with both a young stellar object and a post-AGB star. If confirmed, this would be the closest ever detected bipolar outflow source to the supermassive black hole. Similar to the case of the DSO/G2 source and other dusty sources, it further supports the in situ star formation in the direct vicinity of Sgr A*. If X8 were a bow-shock source, it would be the third object of this type that can be found in projection in the mini-cavity. This scenario would support the idea that the cavity is created by a wind from Sgr A*.
Doppler Sonography during Pregnancy – DEGUM Quality Standards and Clinical Applications (Part 1)
Authors: Faber R. , Heling Kai-Sven , Steiner Horst , Gembruch Ulrich 
Published: March 2019

AbstractDoppler sonography is widely established in prenatal medicine and obstetrics and is commonly used in screening for aneuploidy and preeclampsia during the first trimester. More importantly, during the 2nd and 3rd trimester, it is used in the assessment of fetal health as well as the surveillance of underlying fetal conditions such as IUGR and anemia. Correct use of the method is vital for correct data interpretation and the inferred clinical decision process. Therefore, we aim to provide all users of doppler sonography with a guide for meeting the required quality standards. These quality standards will further be the basis of DEGUM-certified training courses. In the first part, we will introduce the technical principles of the method and potential error sources, vascular disciplines to which the method can be applied, analysis of the spectral curves, patient safety and the needed requirements for successful certification.