bluuebunny/crossref_metadata_2025_split huggingface dataset:
Macro‐ and microelements in pumpkin seed oils: Effect of processing, crop season, and country of origin
Authors: Martinec Nenad , Balbino Sandra , Dobša Jasminka , Šimunić‐Mežnarić Vesna , Legen Saša 
Published: April 2019

AbstractMacro‐ and microelements in the samples of virgin and cold pressed pumpkin seed oils produced in Croatia through two consecutive crop seasons were determined by inductively coupled plasma–optical emission spectroscopy (ICP‐OES). Croatian oils were also compared to oils from Slovenia and Austria in order to assess differences in the element content. Magnesium, potassium, calcium, sodium, selenium, and iron were the dominant elements in all pumpkin seed oils. Their amounts together with barium, strontium, manganese, copper were up to ninefold higher (p ≤ 0.05) in virgin compared to cold pressed pumpkin seed oils. These differences occur due to the different processing conditions which include salt addition, heat treatment, and higher degree of equipment ware out during virgin pumpkin seed oil production. As the sodium level increases with the addition of salt, virgin pumpkin seed oil could be considered its hidden source and producers should pay attention to the amount added. Contents of cobalt, copper, selenium, and thallium significantly differed (p ≤ 0.05) between the two crop seasons. Principal component analysis revealed clear differences between samples with different origin that can be explained by the specifics in the production processes of each country. In comparison with Austrian and Slovenian, Croatian pumpkin seed oils had significantly lower contents of sodium, potassium, calcium, magnesium, and tin while bismuth and selenium were higher.
Mental health stigma in depressed Latinos over the course of therapy: Results from a randomized controlled trial
Authors: Collado Anahi , Zvolensky Michael , Lejuez Carl , MacPherson Laura 
Published: April 2019

AbstractObjectiveThe current study examined the course, correlates, and predictors of mental health stigma among depressed, Spanish‐speaking Latinos that were receiving treatment. This population faces significant disparities in mental health treatment and carries high levels of mental health stigma.MethodThe study utilized data generated from a randomized clinical trial (N = 46) that evaluated the efficacy of Behavioral Activation and Supportive Counseling for depression among Latinos.ResultsMental health stigma decreased over time; these decreases were more pronounced among individuals who were randomized to Supportive Counseling. Mental health stigma was positively associated with depressive symptoms and therapeutic alliance over time. Mental health stigma was not related to treatment attrition.ConclusionsThese preliminary findings indicate that mental health stigma continues to be relevant among individuals who are actively participating in treatment. Receiving mental health treatment may be sufficient to dispel some of the stigmatizing views endorsed by underserved clinical populations.
microRNA expression profiles in two‐ and three‐dimensional culture conditions of human‐umbilical‐cord blood‐derived CD34+ cells
Authors: Arabkari Vahid , Amirizadeh Naser , Nikougoftar Mahin , Soleimani Masoud 
Published: April 2019

AbstractHuman umbilical cord blood (HUCB) is a suitable source of hematopoietic stem cells (HSCs) for therapeutic transplantation. Different approaches have been used to expand the number of HSCs to increase the rate of HSC transplantation success in patients, such as using different cocktails of cytokines, feeder cell layers, and biocompatible scaffolds. microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression posttranscriptionally. They play crucial roles in hematopoiesis including stem cell proliferation, differentiation, stemness, and self‐renewal properties. Here, we studied the UCB‐derived CD34+ cell expansion and the miRNA signatures of CD34+ cells on two‐ and three‐dimensional (2D and 3D) culture conditions. We successfully expanded the UCB‐derived CD34+ cells in both liquid culture (2D) and on aminated polyethersulfone nanofiber scaffolds (3D). Next, we identified the miRNA signature of CD34+ cells and their target genes. We found 58 dysregulated miRNAs in 3D culture condition and 34 dysregulated miRNAs in 2D culture condition when compared to the freshly isolated CD34+ cells. Various types of target genes were also predicted in both conditions using two online databases.
A novel mechanism of Smads/miR‐675/TGFβR1 axis modulating the proliferation and remodeling of mouse cardiac fibroblasts
Authors: Wang Lei , Jiang Ping , He Yi , Hu Hongyu , Guo Yuan , Liu Xiangyang , Qiu Haihua , Ma Qilin , Ouyang Fan 
Published: April 2019

AbstractCardiac fibroblasts (CFs) can over‐proliferate during the progression of cardiac fibrosis, accompanied by a net accumulation of extracellular matrix proteins. Based on the profibrotic actions of transforming growth factor beta 1 (TGFβ1), investigating the mechanisms of TGFβ1 function in CFs may provide new directions to treatment for cardiac fibrosis. microRNAs (miRNAs) could control CFs proliferation or remodeling via binding to 3′‐untranslated region of messenger RNA (mRNA) to negatively regulate gene expression. In the present study, we downloaded several microarray analyses results from GEO attempting to identify miRNAs and possible downstream targets that may be involved in TGF‐β1 function in CFs and to detect the cellular and molecular functions of the identified miRNA–mRNA axis. Here, we identified miR‐675 as a downregulated miRNA by TGFβ1 by bioinformatics analyses and experimental verification. Upon TGFβ1 stimulation, the protein levels of Α‐SMAΑ‐SMA, collagen I, and POSTN, and the secreted collagen in the cell culture supernatant significantly increased, whereas the miR‐675 expression decreased. Smads mediate TGFβ1‐induced suppression on miR‐675 via binding miR‐675 promoter region. miR‐675 overexpression could inhibit, whereas miR‐675 inhibition could enhance TGFβ1‐induced mouse CFs (MCF) remodeling and proliferation. TGFβ receptor 1 (TGFβR1), a critical receptor in TGFβ1/Smad signaling, is a direct downstream target of miR‐675. TGFβR1 overexpression significantly reverses the effect of miR‐675 overexpression on MCF remodeling and proliferation. In summary, miR‐675 targets TGFβR1 to attenuate TGFβ1‐induced MCF remodeling and proliferation. We demonstrate a novel mechanism of the Smads/miR‐675/TGFβR1 axis modulating TGFβ1‐induced MCF remodeling and proliferation.
Does esophageal wall thickness on computed tomography predict response to endoscopic dilatation in patients with corrosive esophageal strictures?
Authors: Gupta Pankaj , Gulati Ajay , Reddy Yalaka R , Samanta Jayanta , Kochhar Rakesh 
Published: April 2019

Background and AimTo evaluate the role of esophageal wall thickness (EWT) on computed tomography (CT) in predicting response to endoscopic dilatation of corrosive esophageal strictures.MethodsThis was a retrospective study. A review of the records of patients who underwent endoscopic dilatation of esophageal strictures between January 2010 and December 2017 was performed. Patients who had a CT evaluation prior to dilatations were included. CT‐EWT was measured at the maximum visible point. Clinical details and endoscopic dilatation parameters were recorded. Technical success, clinical success, and recurrent and refractory strictures were recorded. CT‐EWT and the clinical parameters were evaluated regarding their role in predicting the number of dilatations required to achieve technical and clinical success.ResultsA total of 250 patients underwent endoscopic dilatations during the study period; 84 patients underwent thoracoabdominal CT. Complete clinical, endoscopic, CT data and follow up were available for 64 patients. There were 36 males. The median age was 30 years (range, 14–70 years). A total of 750 dilatations were performed. The median number of dilatations required to achieve technical success was 8.5 (range, 1–51). Dilatations were performed after a median period of 3 months (range, 1–40). Median CT‐EWT was 7 mm (range, 3–22). On univariate, as well as multivariate, analysis, CT‐EWT and the clinical parameters were found to be poor predictors of the number of dilatations required to achieve technical and clinical success.ConclusionCT‐EWT has no additional role in predicting response to the endoscopic dilatation of corrosive esophageal strictures.
High success rates for the use of ombitasvir/paritaprevir/ritonavir containing regimens in treatment of naïve and experienced chronic hepatitis C genotype 4: Real world results
Authors: El Kassas Moham﻿ed , Alboraie Mohamed , Omar Heba , El Latif Yasmeen Abd , Algaber Mohammed Abd , El Tahan Adel , El Halwagy Hesham , Afify Shimaa﻿ , Elserafy Magdy , Elsaeed Kadry , Doss Wahid 
Published: April 2019

AbstractIntroduction and AimsTreatment of hepatitis C virus (HCV) genotype 4 patient with fixed dose combination of ombitasvir‐paritaprevir‐ritonavir plus ribavirin (OBV/rPTV/RBV) has been proven efficacy and safety in many clinical trials. The current study reports the efficacy and safety of OBV/rPTV/RBV (for treatment‐naïve), and OBV/rPTV/RBV/sofosbuvir (SOF) (for treatment‐experienced), in chronic HCV genotype 4 patients in real life settings.MethodsProspective cohort study including all adult chronic HCV genotype 4 patients who were scheduled to receive OBV/rPTV/RBV ± SOF for 12 or 24 weeks in New Cairo Viral Hepatitis Treatment Center. The primary efficacy endpoint was a virologic response at posttreatment week 12 (SVR12). Changes in hematological parameters, liver biochemical profile and fibrosis‐4 index (FIB‐4), as well as clinical and laboratory adverse events (AEs) across follow up visits (week 4, end of treatment [EOT], and SVR12), were recorded.ResultsOur study included 325 patients (age; 47.63 ± 12.63 years, 55.38% [n = 180] men). Most of the included patients (89.85%, n = 292) were treatment naïve and only 7% (n = 23) had liver cirrhosis. Overall, SVR12 was attained by 98.44% (316 of 321) of the patients; 97.15% (307 of 316) of patients who received 12 weeks of OBV/rPTV/RBV ± SOF and 100% (9 of 9) of patients who received 24 weeks of OBV/rPTV/RBV as assessed by modified intention to treat analysis. There was a significant improvement of baseline alanine aminotransferase, aspartate aminotransferase, hemoglobin, FIB‐4 at SVR12 (P < 0.05). The most common reported AEs were anemia (n = 106), fatigue (n = 41) and elevated indirect bilirubin (n = 37).ConclusionOBV/rPTV/RBV (±SOF) is a highly effective therapy for chronic HCV patients in real life settings.
Soybean isoflavones reduce citrinin production by Monascus aurantiacus Li AS3.4384 in liquid state fermentation using different media
Authors: Huang Zhibing , Zhang Lijuan , Gao Heng , Wang Yanling , Li Xiujiang , Huang Xinyu , Huang Ting 
Published: April 2019

AbstractBACKGROUNDMonascus, a filamentous fungus, produces many bioactive substances. However, in the process of fermentation, Monascus also produces the mycotoxin citrinin. Owing to the presence of citrinin, the safety of Monascus products has been questioned and their wide application limited. Using soybean isoflavones (SI) as exogenous additives, alterations in citrinin production by Monascus aurantiacus Li AS3.4384 (MALA) in different media used for liquid state fermentation were investigated.RESULTSResults showed that the citrinin concentration was 95.98% lower than that of the control group after 16‐days fermentation when 20.0 g L−1 SI were added to rice powder and inorganic salt medium. Citrinin production was reduced by 97.24% after 12‐days fermentation with 10.0 g L−1 SI in starch inorganic salt medium; 82.52% after 20‐days fermentation with 20.0 g L−1 SI in starch peptone medium with high starch content; 45.07% after 14‐days fermentation with 5.0 g L−1 SI in starch peptone medium with low starch content; and 82.21% after 14‐days fermentation with 20.0 g L−1 SI in yeast extract sucrose medium.CONCLUSIONThe developed method of removing citrinin is simple, safe, and effective, and it can be applied to reduce the citrinin content of Monascus products. © 2019 Society of Chemical Industry
Detailed methylation map of LINE‐1 5′‐promoter region reveals hypomethylated CpG hotspots associated with tumor tissue specificity
Authors: Sharma Amit , Jamil Muhammad A. , Nuesgen Nicole , Dauksa Albertas , Gulbinas Antanas , Schulz Wolfgang A. , Oldenburg Johannes , El‐Maarri Osman 
Published: April 2019

AbstractBackgroundLong interspersed nuclear elements (LINE‐1) sequences constitute a substantial portion of the human genome, and their methylation often correlating with global genomic methylation. Previous studies have highlighted the feasibility of using LINE‐1 methylation to discriminate tumors from healthy tissues. However, most studies are based on only a few specific LINE‐1 CpG sites.MethodsHerein, we have performed a systematic fine‐scale analysis of methylation at 14 CpGs located in the 5′‐region of consensus LINE‐1, in bladder, colon, prostate, and gastric tumor tissues using a global degenerate approach.ResultsOur results reveal variable methylation levels between different CpGs, as well as some tissue‐specific differences. Trends toward hypomethylation were observed in all tumors types to certain degrees, showing statistically significance in bladder and prostate tumors. Our data points toward the presence of unique LINE‐1 DNA methylation patterns for each tumor type and tissue, indicating that not the same CpGs will be informative for testing in all tumor types.ConclusionThis study provides an accurate guide that will help to design further assays that could avoid artifacts and explain the variability of obtained LINE‐1 methylation values between different studies.
Influences of operator head posture and protective eyewear on eye lens doses in interventional radiology: A Monte Carlo Study
Authors: Mao Li , Liu Tianyu , Caracappa Peter F. , Lin Hui , Gao Yiming , Dauer Lawrence T. , Xu X. George 
Published: April 2019

PurposeTo quantify the effects of operator head posture and different types of protective eyewear on the eye lens dose to operators in interventional radiology (IR).MethodsA deformable computational human phantom, Rensselaer Polytechnic Institute (RPI) Adult Male, consisting of a high‐resolution eye model, was used to simulate a radiologist who is performing an interventional radiology procedure. The radiologist phantom was deformed to a set of different head postures. Three different protective eyewear models were incorporated into the posture‐deformed radiologist phantom. The eye lens dose of the radiologist was calculated using the Monte Carlo code, MCNP. Effects of the radiologist's head posture and different types of protective eyewear on eye lens doses were studied. The relationship between efficacy of protective eyewear and the radiologist's head posture was investigated. Effects of other parameters on efficacy of protective eyewear were also studied, including the angular position of the radiologist, the gap between the eyewear and the face of the radiologist, and the lead equivalent thickness.ResultsThe dose to both lenses decreased by 80% as the head posture moved from looking downward to looking upward. Sports wrap glasses were found to reduce doses further than the other two studied models. The efficacy of eyewear was found to be related to radiologist's head posture as well. When the radiologist was looking up, the protective eyewear almost provided no protection to both lenses. Other factors such as the face‐to‐eyewear distance and the lead equivalent thickness were also found to have an impact on the efficacy of protective eyewear. The dose reduction factor (DRF), defined as the ratio of the dose to the lens without protection to that with protection, decreased from 4.25 to 1.07 as the face‐to‐eyewear distance increased. The DRF almost doubled when the lead equivalent thickness increased from 0.07 to 0.35 mm. However, further increase in lead equivalent thickness showed little improvement in dose reduction.ConclusionThe radiologist's head posture has a significant influence on the eye lens dose in IR. Sports wrap protective eyewear which conforms to the curve of the face is essential for the radiation protection of the eye lens. However, the radiologist's head posture and other exposure parameters should be considered when evaluating the protection of the radiologist's eyes.
Parental Education and Genetics of BMI from Infancy to Old Age: A Pooled Analysis of 29 Twin Cohorts
Authors: Silventoinen Karri , Jelenkovic Aline , Latvala Antti , Yokoyama Yoshie , Sund Reijo , Sugawara Masumi , Tanaka Mami , Matsumoto Satoko , Aaltonen Sari , Piirtola Maarit , Freitas Duarte L. , Maia José A. , Öncel Sevgi Y. , Aliev Fazil , Ji Fuling , Ning Feng , Pang Zengchang , Rebato Esther , Saudino Kimberly J. , Cutler Tessa L. , Hopper John L. , Ullemar Vilhelmina , Almqvist Catarina , Magnusson Patrik K. E. , Cozen Wendy , Hwang Amie E. , Mack Thomas M. , Willemsen Gonneke , Bartels Meike , van Beijsterveldt Catharina E. M. , Nelson Tracy L. , Whitfield Keith E. , Sung Joohon , Kim Jina , Lee Jooyeon , Lee Sooji , Llewellyn Clare H. , Fisher Abigail , Medda Emanuela , Nisticò Lorenza , Toccaceli Virgilia , Baker Laura A. , Tuvblad Catherine , Corley Robin P. , Huibregtse Brooke M. , Derom Catherine A. , Vlietinck Robert F. , Loos Ruth J. F. , Knafo‐Noam Ariel , Mankuta David , Abramson Lior , Burt S. Alexandra , Klump Kelly L. , Silberg Judy L. , Maes Hermine H. , Krueger Robert F. , McGue Matt , Pahlen Shandell , Gatz Margaret , Butler David A. , Harris Jennifer R. , Nilsen Thomas S. , Harden K. Paige , Tucker‐Drob Elliot M. , Franz Carol E. , Kremen William S. , Lyons Michael J. , Lichtenstein Paul , Jeong Hoe‐Uk , Hur Yoon‐Mi , Boomsma Dorret I. , Sørensen Thorkild I. A. , Kaprio Jaakko 
Published: April 2019

ObjectiveThe objective of this study was to analyze how parental education modifies the genetic and environmental variances of BMI from infancy to old age in three geographic‐cultural regions.MethodsA pooled sample of 29 cohorts including 143,499 twin individuals with information on parental education and BMI from age 1 to 79 years (299,201 BMI measures) was analyzed by genetic twin modeling.ResultsUntil 4 years of age, parental education was not consistently associated with BMI. Thereafter, higher parental education level was associated with lower BMI in males and females. Total and additive genetic variances of BMI were smaller in the offspring of highly educated parents than in those whose parents had low education levels. Especially in North American and Australian children, environmental factors shared by co‐twins also contributed to the higher BMI variation in the low education level category. In Europe and East Asia, the associations of parental education with mean BMI and BMI variance were weaker than in North America and Australia.ConclusionsLower parental education level is associated with higher mean BMI and larger genetic variance of BMI after early childhood, especially in the obesogenic macro‐environment. The interplay among genetic predisposition, childhood social environment, and macro‐social context is important for socioeconomic differences in BMI.
Cr concentration driving the structural, mechanical, and thermodynamic properties of Cr‐Al compounds from first‐principles calculations
Authors: Pan Yong 
Published: April 2019

AbstractCr‐Al binary compounds are regarded as the potential high‐temperature structural materials. However, the structure and important properties of Cr‐Al compounds are not completely unclear. Here, we report on the influence of Cr concentration on the structural, mechanical, and thermodynamic properties of Cr‐Al compounds by using the first‐principles calculations. Four novel Cr‐Al compounds, Cr3Al8 with monoclinic structure (C2/m), Cr3Al5 with hexagonal structure (P63mc), Cr2Al3 with tetragonal structure (I4/mmm), and Cr3Al with cubic structure (Pm‐3 m), are predicted. The calculated elastic modulus of Cr‐Al compounds gradually increases with increasing Cr concentration. Compared to other Cr‐Al compounds, our predicted Cr3Al with cubic structure exhibits a strong deformation resistance and high hardness due to symmetrical CrAl bonds. However, the Debye temperature of Cr7Al3 is larger than that of other Cr‐Al compounds. The calculated phonon density of state shows that the high‐temperature thermodynamic properties of Cr‐Al compounds are attributed to the vibration of Al atom and CrAl bond.
Mechanism of injury, injury patterns and associated injuries in patients operated for chest wall trauma
Authors: Caragounis Eva-Corina , Xiao Yao , Granhed Hans 
Published: April 2019

Abstract
Purpose
Chest wall injuries are common in blunt trauma and associated with significant morbidity and mortality. The aim of this study was to determine the most common mechanisms of injury (MOI), injury patterns, and associated injuries in patients who undergo surgery for chest wall trauma.

Methods
This was a retrospective study of trauma patients with multiple rib fractures and unstable thoracic cage injuries who were managed surgically at Sahlgrenska University Hospital during the period September 2010–September 2017. The MOI, injury severity score (ISS), new injury severity score (NISS), thoracic and associated injuries were recorded. Patients were categorized according to age (years): groups I (15‒44), II (45‒64) and III ( > 64). Unstable thoracic cage injuries were classified as sternal, anterior, lateral and posterior flail chest.

Results
Two hundred and eleven trauma patients with a mean age (years) of 58.2 ± 15.6, mean ISS 23.6 ± 11.0, and mean NISS 34.1 ± 10.6 were included in the study. Traffic accidents were the most common MOI in Group I (62%) and falls in Group III (59%). The most common flail segments were lateral and posterior. Sternal and anterior flail segments were more common with bilateral injuries and traffic accidents, particularly frontal collisions. Injuries in at least three body regions were also more associated with traffic accidents. Diaphragmatic injury was seen in 18% of patients who underwent thoracotomy.

Conclusions
The MOI associated with multiple rib fractures differs according to the age of the patient and is associated with different chest wall injury patterns and extra-thoracic injuries.
In/Fertile Monsters: The Emancipatory Significance of Representations of Women on Infertility Reality TV
Authors: de Boer Marjolein Lotte , Archetti Cristina , Solbraekke Kari Nyheim 
Published: April 2019

AbstractReality TV is immensely popular, and various shows in this media genre involve a storyline of infertility and infertility treatment. Feminists argue that normative and constructed realities about infertility and infertility treatment, like those in reality TV, are central to the emancipation of women. Such realities are able to steer viewers' perceptions of the world. This article examines the emancipatory significance of representations of women on 'infertility reality TV shows'. While the women in these shows all have 'abnormal' qualities, we consider their portrayal as figurations of monstrosity. In the literature, monstrosity is understood as a way to challenge nonemancipatory norms by offering an alternative identity. Through a content analysis of seven reality TV shows, we identified four types of in/fertile monsters: the cyborg, the freak, the abject, and the childless. We show that these monsters are predominantly non-emancipatory as they all involve mechanisms of altering, excluding, or condemning infertility in relation to what is considered normal and acceptable womanhood. Therefore, at the end of this article, we make a plea for more diverse and emancipatory representations of infertile women in popular culture.