bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interest of locally weighted regression to overcome nonlinear effects during in situ NIR monitoring of CHO cell culture parameters and antibody glycosylation
Authors: Zavala‐Ortiz Daniel A. , Ebel Bruno , Li Meng‐Yao , Barradas‐Dermitz Dulce Ma. , Hayward‐Jones Patricia M. , Aguilar‐Uscanga Maria G. , Marc Annie , Guedon Emmanuel 
Published: October 2019

AbstractAnimal cell culture processes have become the standard platform to produce therapeutic proteins such as recombinant monoclonal antibodies (mAb). Since the mAb quality could be subject to significant changes depending on manufacturing process conditions, real time monitoring and control systems are required to ensure mAb specifications mainly glycosylation and patient safety. Up to now, real time monitoring glycosylation of proteins has received scarce attention. In this article, the use of near infrared (NIR) to monitor mAb glycosylation has been reported for the first time. Whereas monitoring models are mainly constructed using linear partial least squares regressions (PLSR), evidences presented in this study indicate nonlinearity relationship between in situ captured spectra and compound concentrations, compromising the PLSR performances. A novel and simple approach was proposed to fit nonlinearity using the locally weighted regression (LWR). The LWR models were found to be more appropriate for handling information contained in spectra so that real time monitoring of cultures were accurately performed. Moreover, for the first time, the LWR calibration models allowed mAb glycosylation to be monitored, in a real time manner, by using in situ NIR spectroscopy. These results represent a further step toward developing active‐control feedback of animal cell processes, particularly for ensuring properties of biologics.
Effects of resistant starch type 4 supplementation of bread on in vitro glycemic index value, bile acid‐binding capacity, and mineral bioavailability
Authors: Aribas Merve , Kahraman Kevser , Koksel Hamit 
Published: October 2019

AbstractBackground and objectivesRS4 is an alternative dietary fiber source with some potential physiological benefits giving better quality end products. Common dietary fiber sources including bran usually have deteriorative effect on color, textural properties, and consumer acceptability of breads. This study was designed to investigate the effect of RS4 supplementation on the bread quality as well as mineral bioavailability, bile acid‐binding capacity, in vitro glycemic index.FindingsLoaf volumes of the breads supplemented with RS4 were higher than the bread supplemented with wheat bran (WB). Among the bread samples, the one supplemented with WB had the darkest color and highest firmness values. During storage, WB supplementation caused the highest increase in bread firmness. RS4 supplementation caused higher total dietary fiber (TDF) and mineral bioavailability values and lower in vitro glycemic index than bran supplementation.ConclusionsRS4 supplementation of bread caused increases in TDF content, bile acid‐binding capacity, and mineral bioavailability and has less deteriorative effect on the quality than WB supplementation. Breads supplemented with WB had a faster staling rate than the breads supplemented with RS4.Significance and noveltyBesides the better appearance and texture of the RS4 supplemented bread samples, the improvement in nutritional properties proved that RS4 is a better dietary fiber source than WB.
Defects Engineering in Photocatalytic Water Splitting Materials
Authors: Liu Junying , Wei Zhidong , Shangguan Wenfeng 
Published: October 2019

AbstractDefect engineering has been proved to be an effective approach to improve the photocatalytic performance of the photocatalysts. In this review, recent progress in the design of the defects with the classified anion vacancies, cation vacancies, and multi‐vacancies on the photocatalysts to promote the photocatalytic activity is summarized. The strategies to manufacture the defective photocatalysts and the characterization techniques to distinguish various defects are presented. The roles of defects in photocatalytic water splitting are discussed. Finally, the challenge in developing the defective photocatalysts is outlined.
Follicular cystitis in urine cytology: A clinical and cytopathologic study
Authors: Rohra Prih , Yan Lei , Mir Fatima , Farrell M. Ryan , Gattuso Paolo , Cheng Lin 
Published: October 2019

AbstractDespite being an important differential diagnosis of bladder tumor on cystoscopy, follicular cystitis (FC) is rarely diagnosed on cytologic material. We performed a retrospective study on cases of FC diagnosed on bladder biopsy and/or urine cytology in our institution. A total of 35 cases of FC were identified with a female predominance (F:M = 2:1). Hematuria was the most common clinical presentation. Cystoscopic findings included mass lesions, yellow plaques, and surface erythema. History of urinary tract infection was reported in 48% of the patients, and majority of those patients had positive concurrent urine culture, most commonly with beta‐hemolytic streptococcus, Group B. A total of 17 out of 35 patients had urine cytology specimens. When the presence of follicular dendritic cells in clusters of variously sized lymphocytes is used as the cytological diagnostic criterion, 6 out of 17 cases were diagnosed as FC and 5 out of 6 were confirmed by concurrent biopsy. This retrospective study not only analyzed the clinical characteristics of FC but also elucidated the cytological diagnostic criteria of FC and confirmed its specificity.
Expression of a human variant of CHMP2B linked to neurodegeneration in Drosophila external sensory organs leads to cell fate transformations associated with increased Notch activity
Authors: Wilson Caroline , Kavaler Joshua , Ahmad Syed Tariq 
Published: October 2019

AbstractProper function of cell signaling pathways is dependent upon regulated membrane trafficking events that lead to the endocytosis, recycling, and degradation of cell surface receptors. The endosomal complexes required for transport (ESCRT) genes play a critical role in the sorting of ubiquitinated cell surface proteins. CHMP2BIntron5, a truncated form of a human ESCRT‐III protein, was discovered in a Danish family afflicted by a hereditary form of frontotemporal dementia (FTD). Although the mechanism by which the CHMP2B mutation in this family causes FTD is unknown, the resulting protein has been shown to disrupt normal endosomal–lysosomal pathway function and leads to aberrant regulation of signaling pathways. Here we have misexpressed CHMP2BIntron5 in the developing Drosophila external sensory (ES) organ lineage and demonstrate that it is capable of altering cell fates. Each of the cell fate transformations seen is compatible with an increase in Notch signaling. Furthermore, this interpretation is supported by evidence that expression of CHMP2BIntron5 in the notum environment is capable of raising the levels of Notch signaling. As such, these results add to a growing body of evidence that CHMP2BIntron5 can act rapidly to disrupt normal cellular function via the misregulation of critical cell surface receptor function.
The development of the trunk neural crest in the turtle Trachemys scripta
Authors: Goldberg Sophia , Venkatesh Akshaya , Martinez Jocelyn , Dombroski Catherine , Abesamis Jessica , Campbell Catherine , Mccalipp Mialishia , de Bellard Maria Elena 
Published: October 2019

AbstractBackgroundThe neural crest is a group of multipotent cells that give rise to a wide variety of cells, especially portion of the peripheral nervous system. Neural crest cells (NCCs) show evolutionary conserved fate restrictions based on their axial level of origin: cranial, vagal, trunk, and sacral. While much is known about these cells in mammals, birds, amphibians, and fish, relatively little is known in other types of amniotes such as snakes, lizards, and turtles. We attempt here to provide a more detailed description of the early phase of trunk neural crest cell (tNCC) development in turtle embryos.ResultsIn this study, we show, for the first time, migrating tNCC in the pharyngula embryo of Trachemys scripta by vital‐labeling the NCC with DiI and through immunofluorescence. We found that (a) tNCC form a line along the sides of the trunk NT; (b) The presence of late migrating tNCC on the medial portion of the somite; (c) The presence of lateral mesodermal migrating tNCC in pharyngula embryos; (d) That turtle embryos have large/thick peripheral nerves.ConclusionsThe similarities and differences in tNCC migration and early PNS development that we observe across sauropsids (birds, snake, gecko, and turtle) suggests that these species evolved some distinct NCC pathways.
Sleep problems and psychological symptoms as predictors of musculoskeletal conditions in children and adolescents
Authors: Andreucci Alessandro , Campbell Paul , Richardson Emily , Chen Ying , Dunn Kate M. 
Published: October 2019

AbstractBackgroundPopulation‐based studies show that sleep problems and psychological symptoms may increase the risk of musculoskeletal pain onset in children/adolescents. However, there is no evidence of these associations within primary care settings, where interventions can take place. This study investigated whether children/adolescents who consulted their general practitioner for sleep problems or psychological symptoms are at higher risk of subsequent consultations for musculoskeletal conditions.MethodsThis prospective matched‐cohort study used electronic medical records of children/ adolescents (aged 6–19 years) from a UK primary care database. Associations between a consultation for sleep problems or psychological symptoms at baseline and a subsequent consultation for musculoskeletal conditions within the 2‐year follow‐up were investigated using survival analysis, producing hazard ratio (HR) and 95% confidence interval (CI) with adjustment for confounders.ResultsChildren/adolescents who consulted for sleep problems had a significant increased risk of consultation for musculoskeletal conditions (HR = 1.72; 95% CI 1.14, 2.60), which became nonsignificant after adjustment for confounders (HR = 1.49; 95% CI 0.98, 2.27). Children/adolescents who consulted for psychological symptoms had a significant increased risk of consultation for musculoskeletal conditions (HR = 1.59; 95% CI 1.31, 1.93), which was attenuated after adjustment (HR = 1.39; 95% CI 1.14, 1.70).ConclusionsChildren and adolescents who visit primary care for sleep problems or psychological symptoms have increased risk of future musculoskeletal consultations. Further work is needed to understand the causal mechanisms that explain these associations, before designing interventions strategies within primary care settings.SignificancePopulation‐based studies showed that sleep problems and psychological symptoms may be important precursors of musculoskeletal pain in children and adolescents. By investigating these associations in primary care settings, this study showed that children and adolescents with consultations for sleep problems or psychological symptoms were at increased risk of subsequent consultations for musculoskeletal conditions. These findings support results from population‐based studies, and identify potential areas for further research and potential intervention within primary care.
Energy storage needs for an Australian national electricity market grid without combustion fuels
Authors: Boretti Alberto 
Published: October 2019

AbstractThe opportunity to achieve a wind and solar energy only Australian national electricity market (NEM) grid is linked to the development of massive energy storage systems. Photovoltaic solar energy is unavailable nighttime and reduced when it is cloudy or raining. Wind energy also drops some days to zero, with similarly large fluctuations between maximum and minimum energy supplies. This fundamental aspect is being ignored when only trying to increase the nominal capacity of wind energy and solar photovoltaic facilities. The need for a massive energy storage system to properly use this increased capacity is highlighted by the high‐frequency energy generation data by source that we show for the NEM that is covering most of South Australia, Victoria, Tasmania, New South Wales, and Queensland, with the only exception of Western Australia and the Northern Territory. This analysis highlights the present energy storage needs for a NEM wind and solar only. The nominal capacity of wind and solar, and the energy storage, that is supposed to increase up to unbelievably high values, to only cover the electricity production, is due to increase even more, to cover the TPES demand, and not only the electricity demand, of a country without combustion fuels.
Atomic insights of thin‐film evaporation over biphilic surfaces: Effect of philic‐phobic patterning and wettability contrast
Authors: Hasan Mohammad Nasim , Paul Sudipta , Rownak Md. Ragib , Akhter Chandni 
Published: October 2019

AbstractMolecular dynamics simulations have been carried out to investigate the effect of philic‐phobic patterning and wettability contrast of biphilic (surface with interlaced hydrophilic and hydrophobic segments) surfaces on thin‐film evaporation and compare its performance with the surfaces with uniform wettability, ie, hydrophilic/hydrophobic. Thin liquid film of argon with 3 nm film thickness is placed over platinum surface. After equilibrating the system at 90 K, the temperature of the platinum wall is raised to two different temperatures 110 and 130 K to study the effect of wall superheat temperature. The results obtained in this study indicate that biphilic surface offers wall heat flux and evaporative mass flux close to the hydrophilic surface. With the decrease of philic‐phobic pattern bandwidth of biphilic and superbiphilic surface, the maximum value of evaporative mass flux and wall heat flux increases. Also higher wettability contrast among hydrophilic and hydrophobic region offers higher value of wall heat flux and evaporative mass flux. The boiling inception time decreases with the decrease of philic‐phobic pattern bandwidth and increase of wettability contrast therefore, the nonevaporating layer appears earlier in case of lower philic‐phobic pattern bandwidth and higher wettability contrast.
Bisphenol A aggravates renal ischemia–reperfusion injury by disrupting mitochondrial homeostasis and N‐acetylcysteine mitigates the injurious outcomes
Authors: Peerapanyasut Wachirasek , Kobroob Anongporn , Palee Siripong , Chattipakorn Nipon , Wongmekiat Orawan 
Published: October 2019

AbstractExposure to bisphenol A (BPA), a chemical generally used in consumer products, becomes a global public health concern, as humans are increasingly exposed through their daily consuming activities. Renal ischemia–reperfusion (RIR) is the major cause of acute kidney injury with high prevalence and increased long‐term risks for multiple comorbidities and mortality. As the kidney is susceptible to these conditions, we explored whether the outcomes following the RIR episode could be influenced by BPA exposure, and investigated the therapeutic possibility by N‐acetylcysteine (NAC) including the mechanisms involved. Three groups of male Wistar rats were fed with vehicle, BPA 5, and 50 mg/kg, respectively, for five consecutive weeks then underwent the sham operation. Three other groups with identical treatment underwent bilateral renal IR induction (45‐min ischemia followed by 24‐hr reperfusion). An additional RIR group was treated with BPA 50 plus NAC 100 mg/kg. BPA‐exposed rats that encountered RIR episode showed dose‐dependent worsening of RIR injury as evidenced by augmentations of renal dysfunction and histopathological abnormalities, oxidative stress, apoptosis, mitochondrial functional impairment, mitochondrial dynamic, and mitophagy disproportion compared with the vehicle‐exposed RIR group. The NAC therapy considerably attenuated the exacerbated effects of BPA, which was associated with increased AMP‐activated protein kinase (AMPK), PGC‐1α, silent information regulator 3 or sirtuin 3 (SIRT3), and mitofusin 2 (MFN2) expressions but decreased Phosphorylated dynamin‐related protein 1 (p‐DRP1)/Dynamin‐related protein 1 (DRP1), PTEN‐induced putative kinase (PINK), and PARKIN expressions. These findings reveal the detrimental effect of repeated BPA exposure on the renal outcomes following the IR episode, and further demonstrate the protective efficacy of NAC by maintaining mitochondrial homeostasis, which is, partly, mediated through the AMPK‐PGC‐1α‐SIRT3 axis.
Immobilization of BMP‐2, BMP‐7 and alendronic acid on titanium surfaces: Adhesion, proliferation and differentiation of bone marrow‐derived stem cells
Authors: Kämmerer Peer W. , Pabst Andreas M. , Dau Michael , Staedt Henning , Al‐Nawas Bilal , Heller Martin 
Published: October 2019

AbstractThis study analyzed the influence of titanium (TiO2) surface modifications with two osteogenic proteins (BMP‐2, BMP‐7) and an anti‐osteoclastic drug (alendronic acid [AA]) on sandblasted/acid‐etched (SLA) and plain TiO2 (PT) on cell adhesion, proliferation and differentiation (alkaline phosphatase [AP] and osteocalcin [OC]) of bone‐marrow derived stem cells (BMSCs) after 1, 3 and 7 days in‐vitro. Initially, AA surfaces showed the highest cell number and surface coverage. At day 3 and 7, BMP and AA‐modified surfaces exhibited a significantly enhanced cell growth. For proliferation, at days 3 and 7, an enhancement on BMP‐2, BMP‐7 and AA‐surfaces was seen. At day 7, SLA also showed a higher proliferation when compared to PT. Initially, AP expression was elevated on SLA and AA surfaces. At days 3 and 7, a significant increased AP expression was seen for SLA, BMP‐2, BMP‐7 and AA discs. For OC, SLA and AA surfaces had the highest expression after 1 day whereas after 3 and 7 days a significant difference was recorded for SLA, BMP‐2, BMP‐7 and AA. In conclusion, a beneficial biological effect of a chemical immobilization method of BMP‐2, BMP‐7 and alendronate onto titanium surfaces on BMSCs was proven.
Correlation of 5‐HTR6 gene polymorphism with vestibular migraine
Authors: Wu Xia , Qiu Feng , Wang Zhiwei , Liu Bing , Qi Xiaokun 
Published: October 2019

AbstractObjectiveTo investigate the correlation of 5‐hydroxy tryptamine receptor 6 (5‐HTR6) gene polymorphism with vestibular migraine (VM).MethodsA total of 92 VM patients were enrolled as the observation group, and 100 healthy people receiving physical examinations as the control group. Their general clinical information was collected, and the level of 5‐HT in plasma and the vestibular function test indexes were detected. Moreover, the polymorphism of 5‐HTR6 rs770963777 was detected with the TaqMan‐MGB probe.ResultsThe observation group had a lower level of 5‐HT than the control group (P < .05), and the abnormality rates of the vestibular function tests, including the caloric test, head‐shaking test, and vestibular autorotation test, were obviously higher than those in the control group (P < .01). The comparisons showed that the distribution frequencies of the genotypes and alleles were different between the two groups (P < .05). According to the analysis of the genetic mode, there were differences in recessive and additive modes between the two groups (P < .05), but the dominant mode was not different between the two groups (P > .05).ConclusionThe level of 5‐HT and the vestibular function test indexes can serve as the effective indicators for observing VM, and the polymorphism of 5‐HTR6 rs770963777 site is correlated with VM onset.
Evaluation of a fluorescent immunochromatography test for fecal calprotectin
Authors: Li Runqing , Zhao Xiuying , Dong Jingxiao , Zhu Dong , Wang Tengjiao , Yang Song , Zhao Zhipeng , Xiao Nan 
Published: October 2019

AbstractBackgroundFecal calprotectin (FC) is widely used to discriminate between patients with inflammatory diseases such as inflammatory bowel disease (IBD) and functional diseases such as irritable bowel syndrome (IBS). ELISA is a time‐consuming method for the measurement of FC, whereas a fluorescent immunochromatography test can obtain results in around 30 minutes and thus enables a rapid response to clinical decision.MethodsTwo methods, the Proglead® calprotectin (FC Proglead) and the BÜHLMANN fCAL® ELISA (FC BÜHLMANN), were used to quantitatively examine FC in 111 stool samples. The comparison and bias estimation of both assays were assessed using CLSI EP09c protocol.ResultsThe two methods were highly correlated (rho = .96). Deming regression was employed to calculate the regression equation, with a slope of 1.01 and an intercept of −4.98 μg/g. The estimated median bias (FC Proglead − FC BÜHLMANN) was −4.19 μg/g with the 95% limits of agreement (−55.59 to 47.21 μg/g), and the estimated median percent bias was −8.71% with the 95% limits of agreement (−50.31% to 32.90%). There was 4.50% (5/111) of values outside the 95% limits of agreement. Percent biases at the FC cutoff values of 50 and 200 μg/g between both methods evaluated by Deming regression were 8.96% and 1.49%, respectively. The biases were all less than the acceptable standard (10%). And, 99.10% of FC results were in agreement between both methods (kappa = .99, P < .001).ConclusionsFC Proglead may be used as a suitable alternative to FC BÜHLMANN for the disease activity assessment for patients with IBD, considering its convenience and shorter turnaround time.
Enhanced butanol production by Clostridium acetobutylicum fermentation using thermo‐responsive poly(N‐isopropylacrylamide)‐grafted PDMS sponge as adsorbent material
Authors: Shu Chin‐Hang , Zou Wei‐Lun , Nirwana Wa Ode Cakra 
Published: October 2019

AbstractBACKGROUNDButanol production through ABE (acetone, butanol, and ethanol) fermentation has attracted a great deal of attention because of its potential to replace petroleum‐based fuels. Low butanol production and productivity due to butanol inhibition are the main challenges to make ABE fermentation viable economically. In this work, polydimethylsiloxane (PDMS) sponge and thermos‐responsive poly(N‐isopropylacrylamide)‐grafted PDMS (PNIPAAm‐PDMS) sponges (with different mass fractions of PNIPAAm on the PDMS surface) were investigated as adsorbents for butanol.RESULTSThe butanol model solutions showed that the adsorption–desorption efficiency of different types of sponges for butanol is highly temperature‐dependent. Higher temperatures exhibited higher adsorption efficiency. In contrast, lower temperatures markedly increased the desorption efficiency for butanol. PNIPAAm‐PDMS with 8.0 wt% of PNIPAAm on the PDMS surface showed as the best adsorbent for butanol. It had adsorption efficiency of 14, 24 and 46% at 25, 37 and 50 °C, respectively. Moreover, 96% of butanol adsorbed was recovered at 25 °C. When it was coupled with a batch ABE fermentation by Clostridium acetobutylicum, butanol production increased by 102% compared to batch fermentation without adsorption. A semi‐continuous fermentation with the initial feeding time at the 60th hour exhibited as the optimum condition. However, it failed to enhance butanol productivity due to a high accumulation of butanol in the broth. Then, a semi‐continuous fermentation with adsorption was conducted, yielding total butanol production of 20 g/L.CONCLUSIONPNIPAAm‐PDMS sponge is proposed as an adsorbent to overcome butanol inhibition in ABE fermentation by temperature manipulation to recover butanol. © 2019 Society of Chemical Industry