bluuebunny/crossref_metadata_2025_split huggingface dataset:
c‐Src kinase contributes on endothelial cells mechanotransduction in a heat shock protein 70‐dependent turnover manner
Authors: Pinto Thaís Silva , Fernandes Célio Junior da Costa , da Silva Rodrigo Augusto , Gomes Anderson Moreira , Vieira José Cavalcante Souza , Padilha Pedro de M. , Zambuzzi Willian F. 
Published: December 2018

AbstractShear stress changes are associated with a repertory of signaling cascade modulating vascular phenotype. As shear stress‐related tensional forces might be associated with pathophysiological susceptibility, a more comprehensive molecular map needs to be addressed. Thus, we subjected human umbilical vein endothelial cells (HUVECs) to a circuit of different tensional forces in vitro considering the following three groups: (a) physiological blood flow shear stress condition (named Normo), (b) a hypertensive blood flow shear stress (named Hyper), and (c) these hyper‐stressed cells were returned to Normo condition (named Return). The samples were properly collected to allow different methodologies analysis. Our data showed a pivotal involvement of c‐Src on driving the mechanotransduction cascade by modulating signaling related with adhesion, survival (PI3K/Akt) and proliferative phenotype. Moreover, c‐Src seems to develop important role during extracellular matrix remodeling. Additionally, proteomic analysis showed strong involvement of heat shock protein 70 (HSP70) in the hypertensive‐stressed cells; it being significantly decreased in return phenotype. This result prompted us to investigate 20S proteasome as an intracellular proteolytic alternative route to promote the turnover of those proteins. Surprisingly, our data reveled significant overexpression of sets of proteasome subunit α‐type (PSMA) and β‐type (PSMB) genes. In conjunction, our data showed c‐Src as a pivotal protein to drive mechanotransduction in endothelial cells in a HSP70‐dependent turnover scenario. Because shear patterns is associated with pathophysiological changes, such as atherosclerosis and hypertension, these results paved new road to understand the molecular mechanism on driving mechanotransduction in endothelial cells and, if drugable, these targets must be considered within pharmacological treatment optimization.
Circular RNA hsa_circ_0000263 participates in cervical cancer development by regulating target gene of miR‐150‐5p
Authors: Cai Hongning , Zhang Peng , Xu Meng , Yan Lin , Liu Nian , Wu Xufeng 
Published: December 2018

AbstractCircular RNA (circRNA) is a new class of noncoding RNA, and plays an important role in many pathological processes. Cervical cancer is the most common gynecologic malignant tumor. Recently, studies have shown that there is a variety of circRNA involved in the pathogenesis of cervical cancer. We screened out the highly expressed hsa_circ_0000263 from GSE102686 by the quantitative real‐time polymerase chain reaction assay in cervical cancer cell lines. In this study, we investigated whether hsa_circ_0000263 might affect cell proliferation, migration, cell cycle and apoptosis in cervical cancer in vitro and in vivo. The luciferase reporter assay and RNA immunoprecipitation assay confirmed the direct interaction between miR‐150‐5p and hsa_circ_0000263. By using western blot and immunohistochemistry, we confirmed that hsa_circ_0000263 can regulate the expression of murine double minute 4 (MDM4) by affecting miR‐150‐5p, and finally affect the expression of p53 gene. We found that hsa_circ_0000263 was significantly upregulated in cervical cancer cells. In addition, the knockdown of hsa_circ_0000263, would inhibit cell proliferation and migration ability. In conclusion, our current research reveals the important role of hsa_circ_0000263/miR‐150‐5p/MDM4/p53 regulatory network in cervical cancer and provides a new insight into the pathogenesis of cervical cancer.
Overexpression of cancer‐testis antigen melanoma‐associated antigen A1 in lung cancer: A novel biomarker for prognosis, and a possible target for immunotherapy
Authors: Fanipakdel Azar , Seilanian Toussi Mehdi , Rezazadeh Faezeh , Mohamadian Roshan Nema , Javadinia Seyed Alireza 
Published: December 2018

AbstractAimWe investigated melanoma‐associated antigen A1 (MAGE‐A1) expression in lung cancer tissues and its correlation with prognostic factors.Materials and MethodsIn this cross‐sectional study, samples from 101 patients with lung cancer were obtained between 2007 and 2014 and stained for MAGE‐A1 by immunohistochemistry. Correlation with prognostic factors was assessed by t test, and
χ
2, and Pearson’s tests.ResultsEighty non‐small‐cell lung cancer (NSCLC) and 21 small‐cell lung cancer specimens were stained for MAGE‐A1. MAGE‐A1 was detected more commonly in adenocarcinomas and was expressed more frequently in male and patients >60 years.ConclusionsMAGE‐A1 was found in several lung cancer patients. MAGE‐A1 was expressed more commonly in NSCLC, elderly, and men. Further investigations are needed to assess MAGE‐A1 as potential cancer biomarkers.
The long noncoding RNA uc.294 is upregulated in early‐onset pre‐eclampsia and inhibits proliferation, invasion of trophoblast cells (HTR‐8/SVneo)
Authors: Song Xuejing , Li Chunyan , Li Jingyun , Liu Lan , Meng Li , Ding Hongjuan , Long Wei 
Published: December 2018

AbstractRecently, a large number of long noncoding RNAs (lncRNAs) have been reported in human diseases that are evolutionarily conserved and are likely to play a role in many biological events including pre‐eclampsia. In our previous research, we selected thousands of lncRNAs for their relationship with early‐onset pre‐eclampsia. Among these lncRNAs, a lncRNA named uc.294 attracted our attention, was once reported to specifically be expressed at a high level in the early‐onset of pre‐eclampsia. This study aims to investigate the function of uc.294 in early‐onset pre‐eclampsia and the possible mechanism. The uc.294 expression level in early‐onset pre‐eclampsia or in normal placenta tissues was evaluated by quantitative real‐time polymerase chain reaction. To detect the proliferation, invasion, and apoptosis capacity of the trophoblast cells, we performed the Cell Counting Kit‐8 assay, transwell assay, and flow cytometry, respectively. Here we report, for the first time, that uc.294 inhibits proliferation, invasion, and promotes apoptosis of trophoblast cells HTR‐8/SVneo by working in key aspects of biological behaviors. However, how uc.294 acts to regulate gene functions in early‐onset pre‐eclampsia needs further exploration.
Age‐related changes in the morphology and the distribution of IgA and IgG in the pharyngeal tonsils of yaks (Bos grunniens)
Authors: Xu Yuanfang , Sun Juan , Cui Yan , Yu Sijiu , He Junfeng , Liu Penggang , Zhang Qian 
Published: December 2018

AbstractTo evaluate age‐related changes in the morphology as well as the expression and localization of IgA and IgG in yak pharyngeal tonsils, 20 healthy yaks were divided into four age groups [newborn (1–7 days old), juvenile (5–7 months old), adult (3–6 years old) and old (7–10 years old)]. Morphologic characteristics were observed by histological techniques. The expression and localization of IgA and IgG in pharyngeal tonsils were detected by enzyme linked immunosorbent assay (ELISA) and immunohistochemistry, respectively. The results showed that the epithelium of the pharyngeal tonsils included nonreticular epithelium with an intact basement membrane and reticular epithelium with a discontinuous basement membrane and nonepithelial cell infiltration. In newborn yaks, only primary lymphoid follicles were observed in pharyngeal tonsils. In other age groups, both primary and secondary lymphoid follicles were observed, but some of the lymphoid follicles in the old yaks were degenerated. The number of lymphoid follicles increased from the newborn to the adult group and peaked in the adult group, but the number decreased in the old group. In addition, the age‐related trends of IgA and IgG protein expression were similar to those of the number of lymphoid follicles. The concentration of IgG was significantly higher than that of IgA in all age groups. Both IgA and IgG antibody secreting cells (ASCs) were distributed in the subepithelial region of the nonreticular epithelium, the reticular epithelium, the lymphoid follicles, the interfollicular areas and in between the salivary glands. The densities of IgA and IgG ASCs in pharyngeal tonsils were similar to the expression trend of both proteins in each age group. The results indicate that the morphology and amount of lymphoid follicles in yak pharyngeal tonsils vary with age. Pharyngeal tonsils produce more IgG than IgA, indicating that IgG could be significant component of mucosal immune responses in yaks.
Quantitative histopathologic assessment of perfusion MRI as a marker of glioblastoma cell infiltration in and beyond the peritumoral edema region
Authors: Vallatos A. , Al‐Mubarak H.F.I. , Birch J.L. , Galllagher L. , Mullin J.M. , Gilmour L. , Holmes W.M. , Chalmers A.J. 
Published: December 2018

BackgroundConventional MRI fails to detect regions of glioblastoma cell infiltration beyond the contrast‐enhanced T1 solid tumor region, with infiltrating tumor cells often migrating along host blood vessels.PurposeTo quantitatively and qualitatively analyze the correlation between perfusion MRI signal and tumor cell density in order to assess whether local perfusion perturbation could provide a useful biomarker of glioblastoma cell infiltration.Study TypeAnimal model.SubjectsMice bearing orthotopic glioblastoma xenografts generated from a patient‐derived glioblastoma cell line.Field Strength/Sequences7T perfusion images acquired using a high signal‐to‐noise ratio (SNR) multiple boli arterial spin labeling sequence were compared with conventional MRI (T1/T2 weighted, contrast‐enhanced T1, diffusion‐weighted, and apparent diffusion coefficient).AssessmentImmunohistochemistry sections were stained for human leukocyte antigen (probing human‐derived tumor cells). To achieve quantitative MRI‐tissue comparison, multiple histological slices cut in the MRI plane were stacked to produce tumor cell density maps acting as a “ground truth.”Statistical TestsSensitivity, specificity, accuracy, and Dice similarity indices were calculated and a two‐tailed, paired t‐test used for statistical analysis.ResultsHigh comparison test results (Dice 0.62–0.72, Accuracy 0.86–0.88, Sensitivity 0.51–0.7, and Specificity 0.92–0.97) indicate a good segmentation for all imaging modalities and highlight the quality of the MRI tissue assessment protocol. Perfusion imaging exhibits higher sensitivity (0.7) than conventional MRI (0.51–0.61). MRI/histology voxel‐to‐voxel comparison revealed a negative correlation between tumor cell infiltration and perfusion at the tumor margins (P = 0.0004).Data ConclusionThese results demonstrate the ability of perfusion imaging to probe regions of low tumor cell infiltration while confirming the sensitivity limitations of conventional imaging modalities. The quantitative relationship between tumor cell density and perfusion identified in and beyond the edematous T2 hyperintensity region surrounding macroscopic tumor could be used to detect marginal tumor cell infiltration with greater accuracy.Level of Evidence: 1Technical stage: 2J. Magn. Reson. Imaging 2019;50:529–540.
Value of Cardiac Magnetic Resonance Fractal Analysis Combined With Myocardial Strain in Discriminating Isolated Left Ventricular Noncompaction and Dilated Cardiomyopathy
Authors: Zheng Tian , Ma Xiaohai , Li Shuhao , Ueda Takuya , Wang Zheng , Lu Aijia , Zhou Wei , Zou Hongye , Zhao Lei , Gong Lianggeng 
Published: December 2018

BackgroundExcessive trabeculation is present in isolated left ventricular noncompaction (LVNC) and dilated cardiomyopathy (DCM), which sometimes makes the differentiation between these two difficult. Fractal dimension (FD) is a unitless measure value of how completely the object fills space, which can assess the extent of myocardial trabeculae quantitatively.PurposeTo compare the trabeculae features and myocardial strain derived from cardiac MR between LVNC and DCM.Study TypeRespective case–control series.PopulationIn all, 35 LVNC patients and 30 DCM patients were enrolled, and 20 healthy volunteers were selected as a control group.Field Strength/Sequence5 T with 8‐channel phased‐array cardiac receiver coil including steady‐state free precession cine imaging.AssessmentThe degree of left ventricular trabeculation was evaluated by a semiautomatic tool based on fractal analysis. Myocardial deformation was assessed by feature tracking.Statistical TestsIndependent samples Student's t‐test, Mann–Whitney U‐test, receiver operating characteristics (ROC) curves, and Spearman's rank coefficient were conducted.ResultsMax apical FD and mean global FD were higher in the LVNC group than in the DCM group (1.433 ± 0.074 vs. 1.341 ± 0.062, P < 0.001; 1.323 ± 0.036 vs. 1.267 ± 0.041, P < 0.001, respectively). For diagnosing LVNC, max apical FD was 1.392 (area under the curve [AUC] = 0.881, 95% confidence interval [CI]: 0.804–0.957), and the cutoff value of mean global FD was 1.283 (AUC = 0.895, 95% CI: 0.828–0.961). The global peak longitudinal strain value of the left ventricle (GPLS) showed significant differences between the LVNC group and DCM group [–6.49 (–11.41, –4.90) vs. –4.61 (–5.87, –3.61), P = 0.006]. The diagnostic accuracy for LVNC is highest when using FDs in coordination with GPLS (AUC = 0.93, 95% CI: 0.86–0.98, P < 0.001).Data ConclusionFractal analysis provides a quantitative measurement of myocardial trabeculation. The combination of fractal analysis with myocardial strain provides a novel biomarker in distinguishing LVNC from DCM.Level of Evidence: 3Technical Efficacy Stage: 2J. Magn. Reson. Imaging 2019;50:153–163.
Mixed‐cropping systems of different rice cultivars have grain yield and quality advantages over mono‐cropping systems
Authors: Li Meijuan , Zhang Jiaen , Liu Shiwei , Ashraf Umair , Zhao Benliang , Qiu Shuqing 
Published: December 2018

AbstractBACKGROUNDA mixed‐cropping system that enhances farmland biodiversity has the potential to improve grain yield and quality; however, the impacts of growing different rice cultivars simultaneously has been rarely investigated. In the present study, five popular rice cultivars were selected and ten mixture combinations were made according to the growth period, plant height, grain yield and quality, and pest and disease resistance. Seedlings of the five cultivars and ten mixture combinations (mixed‐sowing of the seeds in an equal ratio, then mixed‐transplanting and finally mixed‐harvesting) were grown in plastic pots in a glasshouse during the early and late growing seasons in 2016.RESULTSCompared with the mono‐cropping systems in the early and late growing seasons in 2016 (paired t‐test), the mixed‐cropping systems increased the rice leaf photosynthetic rate, soil plant analysis development (SPAD) index and total aboveground dry weight. Moreover, mixed‐cropping systems improved the number of spikelets per panicle, seed‐setting rate, and grain weight per pot and harvest index by 19.52% and 5.77%, 8.53% and 4.41%, 8.31% and 4.61%, and 10.26% and 6.98% in the early and late growing seasons, respectively. In addition, mixed‐cropping systems reduced chalky rice rate and chalkiness degree by 33.12% and 43.42% and by 30.11% and 48.13% in the early and late growing seasons, respectively.CONCLUSIONThe SPAD indexes and photosynthetic rates enhanced at physiology maturity in mixed‐cropping systems may result in higher grain yield and better grain quality. In general, it was found that mixed‐cropping with different rice cultivars has the potential for increasing grain yield and improving grain quality. © 2018 Society of Chemical Industry
Second primary lung malignancy following head and neck squamous cell carcinoma
Authors: Crippen Meghan M. , Brady Jacob S. , Burke Lindsay A. , Eloy Jean Anderson , Baredes Soly , Park Richard Chan Woo 
Published: December 2018

Objectives/HypothesisAnalyze the characteristics of second primary lung malignancies (SPLMs) following an index head and neck squamous cell carcinoma (HNSCC).Study DesignRetrospective cohort study.MethodsThe Surveillance, Epidemiology and End Results database was queried for all cases of HNSCC between 1973 and 2014 (N = 101,856). This population was compared to a standard population to assess relative risk for lung cancer, calculated as the standardized incidence ratio (SIR). Patients who developed SPLMs were extracted (N = 8,116) and compared to all other cases of lung cancer (N = 1,160,853) to assess histopathological differences. SPLM subpopulations divided by head and neck primary site were compared for lung cancer histology and time interval between cancer diagnoses.ResultsOverall, 8.0% of HNSCC patients developed SPLMs (SIR = 4.22, P < .001), diagnosed an average of 6.7 years later. Patients with HNSCC of the supraglottis and hypopharynx were at the highest risk relative to a standard population, with SIRs of 8.10 and 6.34, respectively. When comparing SPLMs to all other lung cancers, there was no difference in the distribution of lung lobe affected, but SPLMs were significantly more likely to be of squamous cell carcinoma histology (42.0% vs. 21.0%, P < .001). Among head and neck subsites, lung cancers following larynx tumors had a significantly higher proportion of small cell histology, and those following oropharyngeal or hypopharyngeal tumors had significantly higher proportions of squamous cell histology.ConclusionsPatients who undergo curative treatment of HNSCC are at high risk for developing SPLMs. Subsite‐specific differences may help elucidate the degree of risk attributable to smoking, genetic susceptibility, human papillomavirus infection, or metastasis masquerading as an SPLM.Level of Evidence4 Laryngoscope, 129:903–909, 2019
Pott's puffy tumor in children: A review of the literature
Authors: Koltsidopoulos Petros , Papageorgiou Eleni , Skoulakis Charalampos 
Published: December 2018

ObjectivesPott's puffy tumor (PTT) is a frontal subperiosteal abscess associated with underlying frontal osteomyelitis. The purpose of the present study was to assess the rate of intracranial involvement in pediatric and adolescent patients with PPT and to discuss the diagnostic workup and the therapeutic features of this pathology.MethodsWe searched Web of Science, PubMed and MEDLINE from 1998 to 2018. The search focused on papers concerning the diagnostic procedure and therapeutic management of PTT. Statistical techniques were not used.ResultsWe included 53 articles that described 92 pediatric and adolescent patients with PPT. The overall rate of intracranial complications was found to be 72%. Most authors used computed tomography for the diagnosis of PTT and its complications, either alone or in combination with magnetic resonance imaging. In 50% of cases, an endoscopic endonasal approach is used for the management of the underlying acute or chronic sinusitis.ConclusionsOn the basis of the available literature, it seems that the incidence rate of intracranial involvement in patients with PPT is quite high. Early diagnosis using the appropriate imaging raise the possibility of good recovery. Concerning the therapy of PTT, endoscopic sinus surgery could be considered as a valuable technique. The vast majority of patients treated appropriately recover without long‐term neurologic complications and sequelae.Level of Evidence4 Laryngoscope, 130:225–231, 2020
Molecules targeting the androgen receptor (AR) signaling axis beyond the AR‐Ligand binding domain
Authors: Elshan N. G. R. Dayan , Rettig Matthew B. , Jung Michael E. 
Published: December 2018

AbstractProstate cancer (PCa) is the second most common cause of cancer‐related mortality in men in the United States. The androgen receptor (AR) and the physiological pathways it regulates are central to the initiation and progression of PCa. As a member of the nuclear steroid receptor family, it is a transcription factor with three distinct functional domains (ligand‐binding domain [LBD], DNA‐binding domain [DBD], and transactivation domain [TAD]) in its structure. All clinically approved drugs for PCa ultimately target the AR‐LBD. Clinically active drugs that target the DBD and TAD have not yet been developed due to multiple factors. Despite these limitations, the last several years have seen a rise in the discovery of molecules that could successfully target these domains. This review aims to present and comprehensively discuss such molecules that affect AR signaling through direct or indirect interactions with the AR‐TAD or the DBD. The compounds discussed here include hairpin polyamides, niclosamide, marine sponge–derived small molecules (eg, EPI compounds), mahanine, VPC compounds, JN compounds, and bromodomain and extraterminal domain inhibitors. We highlight the significant in vitro and in vivo data found for each compound and the apparent limitations and/or potential for further development of these agents as PCa therapies.
Technical Note: Use of commercial multilayer Faraday cup for offline daily beam range verification at the McLaren Proton Therapy Center
Authors: Tesfamicael Biniam , Athar Basit , Bejarano Buele Ana , Kozlyuk Valeri , Nichiporov Dmitri , Watts David A. , Arjomandy Bijan 
Published: December 2018

PurposeDaily verification of the proton beam range in proton radiation therapy is a vital part of the quality assurance (QA) program. The objective of this work is to study the use of a multilayer Faraday cup (MLFC) to perform a quick and precise daily range verification of proton beams produced by a synchrotron.MethodsProton beam depth dose measurements were performed at room iso‐center in water using PTW water tank and Bragg Peak ion chamber. The IBA Giraffe, calibrated against the water tank data, was used to measure the water equivalent thickness (WET) of the sample copper plates. The WET measurements provided the range calibration factors for the MLFC. To establish a baseline for in room measurements, range measurements for energies from 70 to 250 MeV in steps of 10 MeV were performed using the Pyramid MLFC at room iso‐center. For the daily range verification measurements, the MLFC is permanently placed at the end of the beam line, inside the accelerator vault. The daily range constancy is performed for five representative beam energies; namely 70, 100, 150, 200, and 250 MeV. Data collected over a period of more than 100 days are analyzed and presented.ResultsThe measured WET values of the copper plates increased with increasing energy. The centroid channel number in the MLFC where the protons stop, was converted to depth in water and compared to the depth of the distal 80% (d80) obtained from the water tank measurements. The depths agreed to within 2 mm, with the maximum deviation of 1.97 mm observed for 250 MeV beam. The daily variation in the ranges measured by the MLFC was within ±0.5 mm. The total time to verify five proton beam ranges varies between 4 and 5 min.ConclusionBased on the result of our measurements, the MLFC can be used for a daily range constancy check with submillimeter accuracy. It is a quick and simple method to perform range constancy verification on a daily basis.