bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gene isolation and expression analysis of galactinol synthase from proso millet (Panicum miliaceum L.)
Authors: Jeevanandhan K. , Rajesh S. , Sujatha K.B. , Dhivya K. , Uma D. 
Published: October 2024

Abstract
Drought is the major devastating stress affecting plant growth and development. The plant develops some physiological and biochemical changes to withstand drought. The raffinose family of oligosaccharides (RFOs) are sugars that have a significant role in abiotic stress tolerance. Galactinol synthase (EC 2.4.1.123; GolS) initiates the primary step of the RFOs synthesis. Their accumulation implies a role for RFOs in abiotic stress adaptation. The galactinol synthase gene from proso millet was isolated and characterized. Full length proso millet galactinol synthase gene of size ~1,200 bp having 3 exons with 2 introns was isolated and predicted using bioinformatics tools. A high sequence similarity was observed at 97.2, 95.6, 91.8 and 85.4% with Panicum hallii, Panicum virgatum, Setaria italica and Zea mays mRNA, respectively. PmGolS gene expression pattern with respect to drought stress was evaluated in proso millet. PmGolS gene was highly expressed, 40.3 and 23.7-fold in vegetative and reproductive stages of drought-stressed plants respectively compared to control. It suggested that PmGolS gene has an active role in drought stress tolerance.
Equatorial Dynamics Significantly Weakened the Southward Somali Current Circulation during 1997–98
Authors: Chen Gengxin 
Published: October 2024

Abstract
Based on reanalysis data and numerical model, this study documented an unusually weak southward Somali Current (SSC) circulation, an anticlockwise circulation in the western Arabian Sea, during the winter of 1997 to spring 1998, throughout the two-decade period from 1994 to 2017. Theoretical analysis and model simulations suggest that the exceptionally weak SSC circulation was primarily caused by abnormal wind forcing around the equator, associated with extreme climate events. The strong equatorial easterly anomalies are mainly responsible for the weakening of the SSC circulation north of ∼6°N and contribute to about half of the weakening south of ∼6°N, by regulating the layer thickness along the eastern boundary, following the time-dependent Sverdrup relation. The large negative wind stress curl anomalies near the equator contribute to another half of the weakening south of ∼6°N. This research underscores the dynamic connection between the circulation in the western basin and equatorial dynamics during extreme climate modes.

Significance Statement
The dynamic linkage between the western basin circulation and the equatorial forcing remains elusive. Based on reanalysis data, theoretical analysis, and numerical simulations, this study has confirmed that the exceptionally weak southward Somali Current circulation during the winter of 1997 to spring 1998 was significantly influenced by abnormal wind patterns around the equator, triggered by extreme climate modes.
A case of Sudden Cardiac Death with myocardial infarction due to iatrogenic coronary dissection in a patient with coronary vasospasm
Authors: Arachchi Samadhi Dandeniya , Sheppard Mary N 
Published: October 2024

Coronary vasospasm and coronary artery dissection are two recognized causes of Sudden Cardiac Death [SCD] in association with Myocardial Infarction in Non-Obstructive Coronary Artery [MINOCA]. This is a case of post procedure death in a 50-year-old female during cardiac angiography, who had a family history of coronary spasm with sudden cardiac death in her mother. She went into cardiac arrest during the procedure with iatrogenic acute coronary dissection which was treated with LMS/LCx vessel stenting. However, she died in 4 days post procedure. Postmortem cardiac examination demonstrated a transmural hemorrhagic infarction of the entire circumferential wall of the left ventricle with normal coronaries both macroscopically and microscopically. Acute coronary dissection was confirmed histologically in the left main stem. Coronary vasospasm is transient constriction of coronary arteries, which causes partial or complete obstruction of the vessels. The exact pathophysiological mechanism is poorly understood, but atheroma, drugs and a genetic predisposition are common associations. This case highlights the strong genetic link of coronary vasospasm in both mother and daughter and also the complication of iatrogenic coronary dissection which is a rare, but serious complication reported in about 0.1% of coronary angiography. This is the first postmortem report of procedure-related dissection in a patient with vasospasm indicating the cause is functional. Forensic practitioners need to be aware of coronary vasospasm and the complications of coronary angiography as a cause of acute myocardial infarction. Detailed autopsy yields valuable information in this rare condition.
Writing Workshops in a Research Methods Course
Authors: LaPaglia Jessica A. 
Published: October 2024

Background Students in research methods courses often engage in the research process and write about their findings in an APA Style report. This process can be intimidating and challenging for students. Objective The purpose of this preliminary study was to examine the use of writing workshops in teaching students to write research reports. Method The Writing Workshop Model included pre-writing worksheets, in-class writing, peer review, and self-evaluation. This method was compared to a more Standard Model that included writing instruction and peer review. Results Students under the Writing Workshop Model had higher scores on the research report than those under the Standard Model. The pre-writing worksheets, in-class writing, and self-evaluation were rated higher than the peer review component of the Writing Workshop Model. Conclusions The Writing Workshop Model may be an effective way to provide support for students during the writing process, thereby enhancing the quality of APA Style research reports. Teaching Implications Instructors of psychology courses could consider incorporating writing workshop components such as pre-writing worksheets, self-evaluations, and in-class writing opportunities to support student writing. Although peer review may be helpful, it is generally unpopular among students.
Efficacy of eptinezumab in non-responders to subcutaneous monoclonal antibodies against CGRP and the CGRP receptor: A retrospective cohort study
Authors: Triller Paul , Blessing Virginia N. , Overeem Lucas H. , Fitzek Mira P. , Hong Ja Bin , Lange Kristin S. , Reuter Uwe , Raffaelli Bianca 
Published: October 2024

Background Migraine patients unresponsive to calcitonin gene-related peptide (CGRP)(-receptor, -R) monoclonal antibodies (mAbs) may benefit from switching between CGRP(-R) mAbs. However, some patients do not tolerate or respond to any subcutaneous mAbs. This study evaluates the efficacy of the intravenous CGRP mAb eptinezumab in these therapy-refractory patients. Methods In this retrospective cohort study, patients with migraine who previously failed erenumab and at least one CGRP mAb (fremanezumab and/or galcanezumab) received eptinezumab 100 mg, followed by a second dose of 100 mg or 300 mg after 12 weeks. Monthly headache days, monthly migraine days, acute medication days, and migraine pain intensity were recorded from standardized headache diaries during the four weeks before the first infusion (baseline), and during weeks 9–12 and 21–24 of treatment. Patient-reported outcomes were analyzed at baseline, weeks 12, and 24. Results From January 2023 to February 2024, 41 patients received eptinezumab 100 mg. Of these, 38 (93%) received a second infusion after 12 weeks, with 29 (71%) increasing the dose to 300 mg. The percentage of patients with a ≥30% reduction rate in monthly migraine days was 23.1% at week 12 and 29.7% at week 24. Monthly migraine days decreased from 16.3 ± 8.0 at baseline to 15.4 ± 8.1 days during weeks 9–12 and 14.4 ± 8.0 days during weeks 21–24 ( p = 0.07). During weeks 21–24, 38.5% reported a clinically meaningful reduction in HIT-6 scores and 52.4% in MIDAS scores. No adverse events were reported. Conclusions Eptinezumab may be an effective and well-tolerated option for some treatment-refractory migraine patients unresponsive to subcutaneous CGRP-(R) mAbs.
Marx-Biased Technical Change and International Profit-Rate Convergence: The Case of Mexican Manufacturing
Authors: Ibarra Carlos A. 
Published: October 2024

The article studies the fall in the output/capital (OC) ratio in Mexican manufacturing, contrasting the hypotheses of high labor costs versus convergence toward the United States as drivers of Marx-biased technical change. While econometric estimations reject the labor-cost hypothesis—as do the evidence from wage-profit curves—they support the convergence one, showing in addition that convergence increased with the degree of US-Mexican industrial integration. Downward OC convergence, moreover, led to downward convergence in profit rates. Convergence aside, capital accumulation itself pushed the OC ratio down, confirming a tendency of accumulation to depress the profit rate. The estimations—for the change in the profit rate and the OC ratio—rely on a comprehensive annual panel of 17 Mexican manufacturing industries over the period 1992–2019. JEL Classification: C23, F15, F63, O14, O33
Surgical Tray Optimization in the Setting of Cleft Lip and Palate Reconstruction: Implications for Operative Cost and Efficiency
Authors: Laspro Matteo , Groysman Leya , Perez Rivera Lucas R. , Geolingo Anna Mae , Tong Henry , Nakos-Athanasiou Marina , Tran David L. , Staffenberg David A. , Flores Roberto L. 
Published: October 2024

Objectives To assess surgical instrument usage in cleft lip (CL) and cleft palate (CP) surgery and create an optimized surgical tray with an associated cost-savings analysis. Design Prospective, observational study. Setting Single institution, 6-month prospective review. Patients/Participants A total of 10 primary CL surgeries and 10 primary CP surgeries were included in this study. Intervention Complete lists of surgical instruments utilized in CL and CP surgeries were collected. Utilization fractions (UFs) were calculated as the percentages of average used instruments to averaged opened instruments per case. New optimized CL and CP surgical trays were idealized by removing instruments not used in at least 20% of cases, and a cost analysis was performed to identify potential savings. Calculation of annual potential savings was also conducted based on institutional caseload. Results The average instrument UFs were 26.0% for CL and 22.6% for CP. The estimated costs were $33.15 to $290.29 for the original CL surgical tray and $10.20 to $63.80 for the optimized tray. For CP, the original tray's cost was estimated at $38.25 to $319.00, and the optimized tray at $9.18 to $57.42. This demonstrates a cost reduction of $22.95 to $226.49 for CL and $29.07 to 261.58 for CP. Conclusions The idealized surgical instrument tray could contribute to reducing healthcare expenditures and promoting operating room efficiency, patient safety, and environmentally friendlier operating theaters.
Safety of the Herbal Medicine SH003 in Patients With Solid Cancer: A Multi-Center, Single-Arm, Open-Label, Dose-Escalation Phase I Study
Authors: Cheon Chunhoo , Lee Hyun Woo , Sym Sun Jin , Ko Seong-Gyu 
Published: October 2024

Background: SH003, a novel herbal medicine comprising Huang-Qi, Dang-Gui, and Gua-Lou-Gen, has historical roots in traditional medicine with reported anticancer properties. The need to explore safe and effective treatments in oncology underlines the importance of this study. Methods: This phase I trial, conducted at Ajou University Hospital and Gachon University Gil Medical Center in Korea, adopted a single-arm, open-label, dose-escalation design. It aimed to evaluate the safety of escalated doses of SH003 in patients with various solid cancers, focusing on determining its maximum tolerated dose. Participants with confirmed solid cancers, unresponsive to standard treatments, were enrolled. The dosage of SH003 was escalated from 4800 to 9600 mg per day, using a 3 + 3 design. Safety was assessed based on the Common Terminology Criteria for Adverse Events ver. 5.0. Results: The study established that the maximum tolerated dose of SH003 is 9600 mg/day. Most adverse events were mild, primarily including dizziness and nausea, indicating the tolerability of SH003 at this dosage. Conclusions: SH003 demonstrates safety and promises as an anticancer treatment at doses up to 9600 mg/day. This research supports further investigation into its efficacy for cancer therapy, emphasizing the significance of natural products in oncology, particularly concerning patient safety and tolerance.
Targeted Muscle Reinnervation Compared to Standard Peripheral Nerve Management Following Amputation: A Systematic Review and Meta-Analysis
Authors: Zimbulis Alexis J. , An Vincent V.G. , Symes Michael , Duraku Liron S. , Gaston R. Glenn , Eberlin Kyle R. , Sivakumar Brahman 
Published: October 2024

Background: Chronic pain remains a significant challenge for individuals following limb amputation, with incidence of painful neuromas, phantom limb pain (PLP), and residual limb pain (RLP). Targeted muscle reinnervation (TMR) is a surgical technique designed to restore motor control information lost during amputation by redirecting residual nerves to new muscle targets. This systematic review and meta-analysis aims to compare patient-reported and functional outcomes following amputation with either TMR or standard neurological treatment (SNT). The study also includes an examination of primary versus secondary TMR and explores outcomes in highly comorbid patient populations. Methods: A search of central databases was performed, and meta-analysis was completed on extracted data where possible. Results: Eleven studies were identified. Results indicate a significant reduction in PLP and RLP in patients undergoing TMR compared to SNT using various pain scores. TMR also demonstrates improved functional outcomes and decreased opioid use. Furthermore, results indicated patients who underwent TMR at the time of amputation (primary TMR) had improved pain scores compared with those who had TMR performed later (secondary TMR). Conclusions: The review emphasizes the benefits of TMR as a valuable surgical adjunct for amputee patients, while also highlighting the need for further research, especially in comorbid populations.
Correlation between physical activity, appetite, and body composition during menopausal transition and postmenopause in Iranian women
Authors: Andarzi Forough , Jahromi Maryam Koushkie 
Published: October 2024

Background: Hormone fluctuations in women are accompanied by an increase in obesity. Although obesity has various causes, the interaction between obesity and menopausal status remains unclear. Objectives: This study aimed to investigate the interaction between lifestyle physical activities (PAs), such as occupational and household activities, and purposeful exercise, such as sports, with appetite and their correlation with body composition during the menopausal transition (MT) and postmenopausal (PM) phases. Design: This was a descriptive cross-sectional design. Methods: Women aged 42–62 years, in the MT or PM phases, were included. PA and appetite were measured using the validated Beacke questionnaire and the Simplified Nutritional Appetite Questionnaire, respectively. Body weight, height, waist circumference, and hip circumference were measured to estimate the body mass index (BMI) and waist-to-hip ratio (WHR). Results: A total of 101 women were included in the study. A significant positive relationship was found between appetite and PA in the MT group ( r = 0.55, p < 0.001). In the MT group, PA (β = −0.38, p = 0.009) and appetite (β = 0.42, p = 0.004) predicted 16% of the BMI, while PA and appetite predicted 16% of the WHR. Only PA contributed significantly to the prediction of WHR (β = −0.33, p = 0.0.02). In the PM group, PA and appetite predicted 15% of the WHR ( p = 0.030), and PA was a significant predictor of the WHR (β = −0.29, p = 0.0.03). There was no significant difference between the BMI and WHR in active women who regularly performed purposeful exercise compared to inactive women during the PM phase ( p > 0.05). However, the appetite of active women was higher than of inactive women ( p = 0.003). Conclusions: Menopausal status was effective on obesity indices, appetite, and PA. Lifestyle PA was superior to appetite in predicting obesity indices during the MT and PM phases, and particularly during PM; hence, PA was more important than exercise in determining obesity.
In situ pinning or modified Dunn procedure? Which one is superior in slipped capital femoral epiphysis surgery? A case–control study including only stable/moderate cases
Authors: Sisman Ali , Poyraz Caner , Akbas Bilal , Cobanoglu Mutlu , Savk Sevki Oner , Cullu Emre 
Published: October 2024

Purpose: The study aimed to compare the clinical and radiologic results of in situ pinning and modified Dunn procedure methods in stable–moderate slipped capital femoral epiphyses surgery. Methods: Slipped capital femoral epiphyses cases between January 2000 and December 2022 were retrospectively analyzed. Stable and moderate cases treated with in situ pinning or modified Dunn procedure and those with a follow-up period longer than 1 year were included. Two groups were formed: the in situ pinning group and the modified Dunn procedure group. Radiologically, postoperative alpha angle, Southwick angle, avascular necrosis, and osteoarthritis rates were compared. Clinically, Harris Hip Score and Merle d’Aubigné score were compared. Total complications were evaluated. Results: The in situ pinning group consisted of 28 patients and the modified Dunn procedure group consisted of 17 patients. The groups were similar in terms of age, gender, affected side, body mass index, Fahey/O’Brien Classification, preoperative slip angles, and follow-up time. Operation time was shorter in the in situ pinning group ( p < 0.001). Postoperative Southwick and alpha angle were lower in the modified Dunn procedure group ( p < 0.001). In clinical outcomes, Merle d’Aubigné and Harris Hip Score were higher in the in situ pinning group ( p = 0.013, p = 0.005, respectively). The rate of avascular necrosis was higher in the modified Dunn procedure group ( p = 0.048). There was no difference between the groups in terms of total complications and osteoarthritis. Conclusions: In situ pinning has an advantage over the modified Dunn procedure in the treatment of stable-moderate slipped capital femoral epiphyses due to shorter operative time, better clinical outcomes, and fewer avascular necrosis rates. Although Southwick and alpha angle measurements were found to be higher after in situ pinning compared to the modified Dunn procedure, this does not constitute a significant disadvantage in terms of osteoarthritis development in the mid-term. Level of Evidence: Level III, case–control study.
Impact of Chondrocyte Inflammation on Glial Cell Activation: The Mediating Role of Nitric Oxide
Authors: Farrag Mariam , Cordero-Barreal Alfonso , Ait Eldjoudi Djedjiga , Varela-García María , Torrijos Pulpón Carlos , Lago Francisca , Essawy Amina , Soffar Ahmed , Pino Jesus , Farrag Yousof , Gualillo Oreste 
Published: October 2024

Objective This study investigates how the inflammatory response of ATDC5 murine chondrogenic cells influences the activity of C6 (rat) and GL261 (mouse) glial cell lines. Prior research suggested nitric oxide (NO) involvement in cartilage-immune crosstalk. The current study explores whether NO, produced by inflamed chondrocytes, mediates signaling between chondrocytes and glial cells. Design Pre-challenged ATDC5 cells with 250 ng/ml of lipopolysaccharide (LPS) were cocultured with GL261 or C6 glioma cells for 24 h with a transwell culture system. Cell viability was assessed using MTT assay. Gene and protein expression were evaluated by qRT-PCR and WB, respectively. Results Real-time reverse transcription-polymerase chain reaction (RT-qPCR) indicated statistically significant upregulation of LCN2, IL-6, TNF-α, IL-1β, and GFAP in glial cells following 24-h coculture with challenged ATDC5 cells. Suppression of LPS-induced NO production by aminoguanidine decreased LPS-mediated LCN2 and IL-6 expression in glioma cells. We identified also the involvement of the ERK1/2 and AKT signaling pathways in the glial neuroinflammatory response. Conclusions This study demonstrates, for the first time, that NO produced by inflamed murine chondrocytes mediated pro-inflammatory responses in glial cells via ERK1/2 and AKT signaling, highlighting a potential mechanism linking cartilage NO to neuroinflammation and chronic pain in osteoarthritis.
Nurses’ knowledge toward hemodialysis vascular access devices: A cross-sectional study in Palestine
Authors: Abu El-Kass Sae’d , Ahmed Nisreen , Kannan Tahreer , Abu Shediq Narmean , El Dirani Esraa 
Published: October 2024

Objective: To assess the nurses’ knowledge of vascular access devices for patients undergoing hemodialysis programs in the Gaza Strip, Palestine. Methods: A descriptive cross-sectional study was conducted to assess the nurses’ knowledge of hemodialysis vascular access using the convenience sampling technique. The assessment tool comprised 60 items related to the nurses’ knowledge and 7 items on demographic characteristics at hemodialysis units in 5 governmental hospitals in the Gaza Strip, between March and June 2023. Statistical analysis of the collected data was performed using SPSS version 22. Results: A total of 65 nurses in hemodialysis units were included. Of the majority of study nurses 71% were male, 21.5% of nurses had fair knowledge, and 78.5% had good knowledge about vascular access devices. The overall average score was more than 70% in all dimensions, which revealed good knowledge about vascular access devices. The study indicated that there was a statistically significant association between nurses’ knowledge of vascular access devices and (years of experience in the hemodialysis unit, and received training of nurses). Conclusions: The knowledge of hemodialysis nurses toward vascular access devices for patients undergoing hemodialysis was good. The years in the hemodialysis unit and having training have shown significant association with knowledge among hemodialysis nurses on vascular access devices care. Thus, interventions should focus on providing training for nurses about vascular access devices care in hemodialysis units to improve the comprehensive knowledge of nurses and to maintain patient’s health status.