bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ingestion of bioactive collagen hydrolysates enhanced pressure ulcer healing in a randomized double-blind placebo-controlled clinical study
Authors: Sugihara Fumihito , Inoue Naoki , Venkateswarathirukumara Sriraam 
Published: July 2018

AbstractWe conducted a double blind, multi-centric, placebo-controlled, randomized trial to compare the Pressure Ulcer Scale for Healing (PUSH) and Pressure Sore Status Tool (PSST) scores and wound area measurements at 16 weeks of subjects with pressure ulcers who were given standard care plus one of two types of collagen hydrolysate (CH-a), which contained low levels of prolylhydroxyproline (Pro-Hyp) and hydroxyprolylglycine (Hyp-Gly), and CH-b, which contained high levels of Pro-Hyp and Hyp-Gly) with the placebo group. A total of 120 subjects with stage II or III pressure ulcers were entered into the trial and 112 subjects completed the study. The subjects were randomized to receive CH-a (n = 39), CH-b (n = 39), or a placebo (n = 42) twice daily (10 g per day) for 16 weeks. The PUSH score, PSST score, and wound area of the CH-b group were significantly lower than the placebo group at week 16 (PUSH score, P < 0.001; PSST score, P < 0.01; wound area, P < 0.05). The PUSH score of the CH-a group was significantly lower than the placebo group at week 16 (P < 0.05). This study demonstrated that CH-b ingestion helps healing of pressure ulcers as an add-on to the standard therapy.
Structural dynamics of the transaminase active site revealed by the crystal structure of a co-factor free omega-transaminase from Vibrio fluvialis JS17
Authors: Shin Young-Cheul , Yun Hyungdon , Park Hyun Ho 
Published: July 2018

AbstractOmega (ω)-transaminase catalyzes the transfer of an amino group from a non-α position amino acid, or an amine compound with no carboxylic group, to an amino acceptor, and has been studied intensively because of its high potential utility in industry and pharmatheutics. The ω-transaminase from Vibrio fluvialis JS17 (Vfat) is an amine:pyruvate transaminase capable of the stereo-selective transamination of arylic chiral amines. This enzyme exhibits extraordinary enantio-selectivity, and has a rapid reaction rate for chiral amine substrates. In this study, we report the crystal structure of the apo form of Vfat. The overall structure of Vfat was typical of other class III aminotransferase exhibiting an N-terminal helical domain, a small domain, and a large domain. Interestingly, the two subunits of apo Vfat in the asymmetric unit had different structures. A comparison of the overall structure to other transaminases, revealed that the structures of the N-terminal helical domain and the large domain can be affected by cofactor occupancy, but the structural rearrangement in these regions can occur independently.
Exploring Cuba’s population structure and demographic history using genome-wide data
Authors: Fortes-Lima Cesar , Bybjerg-Grauholm Jonas , Marin-Padrón Lilia Caridad , Gomez-Cabezas Enrique Javier , Bækvad-Hansen Marie , Hansen Christine Søholm , Le Phuong , Hougaard David Michael , Verdu Paul , Mors Ole , Parra Esteban J. , Marcheco-Teruel Beatriz 
Published: July 2018

AbstractCuba is the most populated country in the Caribbean and has a rich and heterogeneous genetic heritage. Here, we take advantage of dense genomic data from 860 Cuban individuals to reconstruct the genetic structure and ancestral origins of this population. We found distinct admixture patterns between and within the Cuban provinces. Eastern provinces have higher African and Native American ancestry contributions (average 26% and 10%, respectively) than the rest of the Cuban provinces (average 17% and 5%, respectively). Furthermore, in the Eastern Cuban region, we identified more intense sex-specific admixture patterns, strongly biased towards European male and African/Native American female ancestries. Our subcontinental ancestry analyses in Cuba highlight the Iberian population as the best proxy European source population, South American and Mesoamerican populations as the closest Native American ancestral component, and populations from West Central and Central Africa as the best proxy sources of the African ancestral component. Finally, we found complex admixture processes involving two migration pulses from both Native American and African sources. Most of the inferred Native American admixture events happened early during the Cuban colonial period, whereas the African admixture took place during the slave trade and more recently as a probable result of large-scale migrations from Haiti.
Aluminum salts as an adjuvant for pre-pandemic influenza vaccines: a meta-analysis
Authors: Lin Yu-Ju , Shih Yun-Jui , Chen Chang-Hsun , Fang Chi-Tai 
Published: July 2018

AbstractAvian-origin H5/H7 influenza has the potential to cause the next influenza pandemic. Availability of effective vaccines is an essential part of pre-pandemic preparedness. However, avian influenza surface antigens are poorly immunogenic to humans, which necessitates the use of adjuvants to augment the immunogenicity of pre-pandemic influenza vaccines. Aluminum salts are approved, safe, and affordable adjuvants, but their adjuvanticity for influenza vaccines remains unverified. We conducted the first meta-analysis on this issue. A total of nine randomized controlled trials (2006–2013, 22 comparisons, 2,467 participants in total) compared aluminum-adjuvanted H5N1 vaccines versus non-adjuvanted counterparts. The weighted estimate for the ratio of the seroprotection rate after a single dose of H5N1 vaccine is 0.66 (95% CI: 0.53 to 0.83) by hemagglutination-inhibition assay or 0.56 (95% CI: 0.42 to 0.74) by neutralizing titer assay. The weighted estimate for the risk ratio of pain/tenderness at injection sites is 1.85 (95% CI: 1.56 to 2.19). The quality of evidence is low to very low for seroprotection (due to indirectness and potential reporting bias) and moderate for pain/tenderness (due to potential reporting bias), respectively. The significantly lower seroprotection rate after aluminum-adjuvanted H5N1 vaccines and the significantly higher risk of pain at injection sites indicate that aluminum salts decrease immunogenicity but increase local reactogenicity of pre-pandemic H5N1 vaccines in humans.
Reconfigurable and tunable twisted light laser
Authors: Zhou Nan , Liu Jun , Wang Jian 
Published: July 2018

AbstractTwisted light, having a helical spatial phase structure and carrying orbital angular momentum (OAM), has given rise to many developments ranging from optical manipulation to optical communications. The laser excitation of twisted light in a reconfigurable and tunable way is of great interest. Here, we propose and experimentally demonstrate an OAM reconfigurable and wavelength tunable twisted light laser with achievable high-order OAM modes on a hybrid free-space and fiber platform. The excited twisted light laser is enabled by a ring resonator incorporating spatial light modulators (SLMs) and bandpass filter (BPF). By appropriately switching the phase pattern loaded onto SLMs and adjusting the BPF, twisted light laser with reconfigurable OAM and tunable wavelength is implemented. In the experiment, the OAM value is varied from −10 to +10 and the wavelength is adjusted from 1530 to 1565 nm covering the whole C band. The obtained results indicate successful implementation of a reconfigurable and tunable twisted light laser with favorable operation performance. Reconfigurable and tunable twisted light laser may open up new perspectives to more extensive OAM-enabled applications with improved flexibility and robustness.
Combination of chemotherapy and Au-nanoparticle photothermy in the visible light to tackle doxorubicin resistance in cancer cells
Authors: Pedrosa Pedro , Mendes Rita , Cabral Rita , Martins Luísa M. D. R. S. , Baptista Pedro V. , Fernandes Alexandra R. 
Published: July 2018

AbstractDespite great advances in the fight against cancer, traditional chemotherapy has been hindered by the dose dependent adverse side effects that reduce the usable doses for effective therapy. This has been associated to drug resistance in tumor cells that often cause relapse and therapy failure. These drawbacks have been tackled by combining different therapeutic regiments that prevent drug resistance while decreasing the chemotherapy dose required for efficacious ablation of cancer. In fact, new metallic compounds have been in a continuous development to extend the existing chemotherapy arsenal for these combined regimens. Here, we demonstrate that combination of a metallic compound (TS265), previously characterized by our group, with photothermy circumvents cells resistant to Doxorubicin (DOX). We first engendered a colorectal carcinoma cell line (HCT116) highly resistant to DOX, whose viability was diminished after administration of TS265. Cancer cell death was potentiated by challenging these cells with 14 nm spherical gold nanoparticles followed by laser irradiation at 532 nm. The combination of TS265 with photothermy lead to 65% cell death of the DOX resistant cells without impacting healthy cells. These results support the use of combined chemotherapy and photothermy in the visible spectrum as an efficient tool for drug resistant tumors.
Ambient Processed, Water-Stable, Aqueous-Gated sub 1 V n-type Carbon Nanotube Field Effect Transistor
Authors: Joshi Saumya , Bhatt Vijay Deep , Jaworska Ewa , Michalska Agata , Maksymiuk Krzysztof , Becherer Markus , Gagliardi Alessio , Lugli Paolo 
Published: July 2018

AbstractIn this paper we report for the first time an n-type carbon nanotube field effect transistor which is air- and water-stable, a necessary requirement for electrolyte gated CMOS circuit operation. The device is obtained through a simple process, where the native p-type transistor is converted to an n-type. This conversion is achieved by applying a tailor composed lipophilic membrane containing ion exchanger on the active channel area of the transistor. To demonstrate the use of this transistor in sensing applications, a pH sensor is fabricated. An electrolyte gated CMOS inverter using the herein proposed novel n-type transistor and a classical p-type transistor is demonstrated.
Human erythrocytes release ATP by a novel pathway involving VDAC oligomerization independent of pannexin-1
Authors: Marginedas-Freixa Irene , Alvarez Cora Lilia , Moras Martina , Leal Denis María Florencia , Hattab Claude , Halle François , Bihel Frédéric , Mouro-Chanteloup Isabelle , Lefevre Sophie Denise , Le Van Kim Caroline , Schwarzbaum Pablo Julio , Ostuni Mariano Anibal 
Published: July 2018

AbstractWe previously demonstrated that the translocase protein TSPO2 together with the voltage-dependent anion channel (VDAC) and adenine nucleotide transporter (ANT) were involved in a membrane transport complex in human red blood cells (RBCs). Because VDAC was proposed as a channel mediating ATP release in RBCs, we used TSPO ligands together with VDAC and ANT inhibitors to test this hypothesis. ATP release was activated by TSPO ligands, and blocked by inhibitors of VDAC and ANT, while it was insensitive to pannexin-1 blockers. TSPO ligand increased extracellular ATP (ATPe) concentration by 24–59% over the basal values, displaying an acute increase in [ATPe] to a maximal value, which remained constant thereafter. ATPe kinetics were compatible with VDAC mediating a fast but transient ATP efflux. ATP release was strongly inhibited by PKC and PKA inhibitors as well as by depleting intracellular cAMP or extracellular Ca2+, suggesting a mechanism involving protein kinases. TSPO ligands favoured VDAC polymerization yielding significantly higher densities of oligomeric bands than in unstimulated cells. Polymerization was partially inhibited by decreasing Ca2+and cAMP contents. The present results show that TSPO ligands induce polymerization of VDAC, coupled to activation of ATP release by a supramolecular complex involving VDAC, TSPO2 and ANT.
First-principles and Molecular Dynamics simulation studies of functionalization of Au32 golden fullerene with amino acids
Authors: Darvish Ganji M. , Tavassoli Larijani H. , Alamol-hoda R. , Mehdizadeh M. 
Published: July 2018

AbstractWith the growing potential applications of nanoparticles in biomedicine especially the increasing concerns of nanotoxicity of gold nanoparticles, the interaction between protein and nanoparticles is proving to be of fundamental interest for bio-functionalization of materials. The interaction of glycine (Gly) amino acid with Au32fullerene was first investigated with B3LYP-D3/TZVP model. Several forms of glycine were selected to better understand the trends in binding nature of glycine interacting with the nanocage. We have evaluated various stable configurations of the Gly/Au32complexes and the calculated adsorption energies and AIM analysis indicate that non-Gly, z-Gly and also tripeptide glycine can form stable bindings with Au32at aqueous solution via their amino nitrogen (N) and/or carbonyl/carboxyl oxygen (O) active sites. Furthermore, cysteine, tyrosine, histidine and phenylalanine amino acids bound also strongly to the Au32nanocage. Electronic structures and quantum molecular descriptors calculations also demonstrate the significant changes in the electronic properties of the nanocage due to the attachment of selected amino acids. DFT based MD simulation for the most stable complex demonstrate that Gly/Au32complex is quite stable at ambient condition. Ourfirst-principlesfindings offer fundamental insights into the functionalization of Au32nanocage and envisage its applicability as novel carrier of the drugs.
A merged lung cancer transcriptome dataset for clinical predictive modeling
Authors: Lim Su Bin , Tan Swee Jin , Lim Wan-Teck , Lim Chwee Teck 
Published: July 2018

AbstractThe Gene Expression Omnibus (GEO) database is an excellent public source of whole transcriptomic profiles of multiple cancers. The main challenge is the limited accessibility of such large-scale genomic data to people without a background in bioinformatics or computer science. This presents difficulties in data analysis, sharing and visualization. Here, we present an integrated bioinformatics pipeline and a normalized dataset that has been preprocessed using a robust statistical methodology; allowing others to perform large-scale meta-analysis, without having to conduct time-consuming data mining and statistical correction. Comprising 1,118 patient-derived samples, the normalized dataset includes primary non-small cell lung cancer (NSCLC) tumors and paired normal lung tissues from ten independent GEO datasets, facilitating differential expression analysis. The data has been merged, normalized, batch effect-corrected and filtered for genes with low variance via multiple open source R packages integrated into our workflow. Overall this dataset (with associated clinical metadata) better represents the diseased population and serves as a powerful tool for early predictive biomarker discovery.
Pt-like catalytic behavior of MoNi decorated CoMoO3cuboid arrays for the hydrogen evolution reaction
Authors: Wang Dewen , Han Ce , Xing Zhicai , Li Qun , Yang Xiurong 
Published: July 2018

Owing to the unique structure and synergistic effect between MoNi and CoMoO3, the MoNi/CoMoO3/NF exhibits high hydrogen evolution activity which requires a low overpotential of only 18 mV to deliver a current density of 10 mA cm−2.
Knockdown of long non-coding RNA linc00511 suppresses proliferation and promotes apoptosis of bladder cancer cells via suppressing Wnt/β-catenin signaling pathway
Authors: Li Jun , Li Yan , Meng Fandong , Fu Liye , Kong Chuize 
Published: July 2018

More and more studies have shown that long non-coding RNAs (lncRNAs) play critical roles in various biological processes of bladder cancer, including proliferation, apoptosis, migration and cell cycle arrest. LncRNA long intergenic noncoding RNA 00511 (linc00511) is one of the lncRNAs highly expressed in bladder cancer tissues and cells. However, little is known about the roles and mechanisms of linc00511 in bladder cancer. Here, we demonstrated that linc00511 was highly expressed in bladder cancer tissues and cells. Linc00511 knockdown could cause the cell proliferation suppression and cell cycle arrest, which were mediated by p18, p21, CDK4, cyclin D1 and phosphorylation Rb. Suppressed linc00511 could induce the apoptosis in T24 and BIU87 cells via activating the caspase pathway. Down-regulation of linc00511 could also suppress the migration and invasion of T24 and BIU87 cells. In addition, we found that the expression of linc00511 was negatively correlated with that of miR-15a-3p in the clinical bladder cancer samples. Further mechanistic studies showed that linc00511 knockdown induced proliferation inhibition, and apoptosis increase might be regulated through suppressing the activities of Wnt/β-catenin signaling pathway. Thus, we revealed that knockdown of linc00511 suppressed the proliferation and promoted apoptosis of bladder cancer cells through suppressing the activities of Wnt/β-catenin signaling pathway. Moreover, we suggested that linc00511 could be a potential therapeutic target and novel biomarker in bladder cancer.
The Solar Twin Planet Search
Authors: Lorenzo-Oliveira Diego , Freitas Fabrício C. , Meléndez Jorge , Bedell Megan , Ramírez Iván , Bean Jacob L. , Asplund Martin , Spina Lorenzo , Dreizler Stefan , Alves-Brito Alan , Casagrande Luca 
Published: July 2018

Context. It is well known that the magnetic activity of solar-type stars decreases with age, but it is widely debated in the literature whether there is a smooth decline or if there is an early sharp drop until 1–2 Gyr that is followed by a relatively inactive constant phase.
Aims. We revisited the activity-age relation using time-series observations of a large sample of solar twins whose precise isochronal ages and other important physical parameters have been determined.
Methods. We measured the Ca II H and K activity indices using ≈9000 HARPS spectra of 82 solar twins. In addition, the average solar activity was calculated through asteroids and Moon reflection spectra using the same instrumentation. Thus, we transformed our activity indices into the S Mount Wilson scale (SMW), recalibrated the Mount Wilson absolute flux and photospheric correction equations as a function of Teff, and then computed an improved bolometric flux normalized activity index log R′HK (Teff) for the entire sample.
Results. New relations between activity and the age of solar twins were derived by assessing the chromospheric age-dating limits using log R′HK (Teff). We measured an average solar activity of SMW = 0.1712 ± 0.0017 during solar magnetic cycles 23–24 covered by HARPS observations, and we also inferred an average of SMW = 0.1694 ± 0.0025 for cycles 10–24, anchored on a sunspot number correlation of S index versus. We also found a simple relation between the average and the dispersion of the activity levels of solar twins. This enabled us to predict the stellar variability effects on the age-activity diagram, and consequently, to estimate the chromospheric age uncertainties that are due to the same phenomena. The age-activity relation is still statistically significant up to ages around 6–7 Gyr, in agreement with previous works using open clusters and field stars with precise ages.
Conclusions. Our research confirms that Ca II H & K lines remain a useful chromospheric evolution tracer until stars reach ages of at least 6–7 Gyr. We found evidence that for the most homogenous set of old stars, the chromospheric activity indices seem to continue to decrease after the solar age toward the end of the main sequence. Our results indicate that a significant part of the scatter observed in the age-activity relation of solar twins can be attributed to stellar cycle modulations effects. The Sun seems to have a normal activity level and variability for its age.
A collection of model stellar spectra for spectral types B to early-M
Authors: Allende Prieto C. , Koesterke L. , Hubeny I. , Bautista M. A. , Barklem P. S. , Nahar S. N. 
Published: July 2018

Context. Models of stellar spectra are necessary for interpreting light from individual stars, planets, integrated stellar populations, nebulae, and the interstellar medium.
Aims. We provide a comprehensive and homogeneous collection of synthetic spectra for a wide range of atmospheric parameters and chemical compositions.
Methods. We compile atomic and molecular data from the literature. We adopt the largest and most recent set of ATLAS9 model atmospheres, and use the radiative code ASSϵT.
Results. The resulting collection of spectra is made publicly available at medium and high-resolution (R ≡ λ/δλ = 10 000, 100 000 and 300 000) spectral grids, which include variations in effective temperature between 3500 K and 30 000 K, surface gravity (0 ≤ log g ≤ 5), and metallicity (−5 ≤ [Fe/H] ≤ +0:5), spanning the wavelength interval 120–6500 nm. A second set of denser grids with additional dimensions, [α/Fe] and micro-turbulence, are also provided (covering 200–2500 nm). We compare models with observations for a few representative cases.
The Gaia-ESO Survey: The N/O abundance ratio in the Milky Way
Authors: Magrini L. , Vincenzo F. , Randich S. , Pancino E. , Casali G. , Tautvaišienė G. , Drazdauskas A. , Mikolaitis Š. , Minkevičiūtė R. , Stonkutė E. , Chorniy Y. , Bagdonas V. , Kordopatis G. , Friel E. , Roccatagliata V. , Jiménez-Esteban F. M. , Gilmore G. , Vallenari A. , Bensby T. , Bragaglia A. , Korn A. J. , Lanzafame A. C. , Smiljanic R. , Bayo A. , Casey A. R. , Costado M. T. , Franciosini E. , Hourihane A. , Jofré P. , Lewis J. , Monaco L. , Morbidelli L. , Sacco G. , Worley C. 
Published: July 2018

Context. The abundance ratio N/O is a useful tool to study the interplay of galactic processes, for example star formation efficiency, timescale of infall, and outflow loading factor.
Aims. We aim to trace log(N/O) versus [Fe/H] in the Milky Way and to compare this ratio with a set of chemical evolution models to understand the role of infall, outflow, and star formation efficiency in the building up of the Galactic disc.
Methods. We used the abundances from IDR2-3, IDR4, IDR5 data releases of the Gaia-ESO Survey both for Galactic field and open cluster stars. We determined membership and average composition of open clusters and we separated thin and thick disc field stars. We considered the effect of mixing in the abundance of N in giant stars. We computed a grid of chemical evolution models, suited to reproduce the main features of our Galaxy, exploring the effects of the star formation efficiency, infall timescale, and differential outflow.
Results. With our samples, we map the metallicity range −0.6 ≤ [Fe/H] ≤ 0.3 with a corresponding −1.2 ≤ log(N/O) ≤ −0.2, where the secondary production of N dominates. Thanks to the wide range of Galactocentric distances covered by our samples, we can distinguish the behaviour of log(N/O) in different parts of the Galaxy.
Conclusions. Our spatially resolved results allow us to distinguish differences in the evolution of N/O with Galactocentric radius. Comparing the data with our models, we can characterise the radial regions of our Galaxy. A shorter infall timescale is needed in the inner regions, while the outer regions need a longer infall timescale, coupled with a higher star formation efficiency. We compare our results with nebular abundances obtained in MaNGA galaxies, finding in our Galaxy a much wider range of log(N/O) than in integrated observations of external galaxies of similar stellar mass, but similar to the ranges found in studies of individual H II regions.