bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of fixing nitrogen in common bean growth under water deficit conditions
Authors: Fenta Berhanu Amsalu , Beebe Stephen E. , Kunert Karl J. 
Published: July 2019

AbstractIn common bean (Phaseolus vulgaris L), limited information is available if potential of nodulation and symbiotic nitrogen fixation is associated with the expression of traits that confer better adaptation to drought conditions. Adaptive traits were therefore measured in N‐fixing bean lines BAT 477, DOR 364, and PAN 185 and in nonnodulating lines BAT 477‐NN and DOR 364‐NN. Drought affected growth in all bean lines and decreased chlorophyll content but more in DOR 364‐NN and BAT 477‐NN, and also reduced nodule size with highest reduction in DOR 364 (21.6%). Loss of nitrogen fixation under drought conditions was further associated with lower water use efficiency, measured as carbon isotope ratio of 13C/12C, and lower %N in shoots when compared to capability to fix nitrogen. Loss of symbiotic nitrogen fixation in mutant bean lines DOR 364‐NN and BAT 477‐NN negatively affected root architectural traits under drought. Under drought conditions, line BAT 477‐NN had about 50% lower values for all root architectural traits, such as root angle, strongly emphasizing an association of capability to fix nitrogen with root development. An association between capability to fix nitrogen and maintaining a better harvest index and seed yield was also found in the study. Under well‐watered conditions, all N‐fixing lines had a higher harvest index and seed yield (2.6–2.8 t of seed yield/ha) in comparison with the two nonfixing bean lines (1.4 t/ha). Although the harvest index and seed yield declined due to drought in all tested lines, this decline was only significant for line DOR 364‐NN (harvest index) and lines DOR 364‐NN and BAT 477‐NN (seed yield). Our study has overall demonstrated an important association between the capability of maintaining atmospheric N‐fixation and expression of traits conferring better adaptation to drought conditions with any change in nitrogen fixation affecting these traits.
Temporomandibular disorder in head and neck cancer patients undergoing radiotherapy: Clinical findings and patient‐reported symptoms
Authors: Pauli Nina , Mejersjö Christina , Fagerberg‐Mohlin Bodil , Finizia Caterina 
Published: July 2019

AbstractBackgroundThe aim of this study was to investigate how common temporomandibular disorder (TMD) symptoms are among head and neck cancer (HNC) patients before and after oncological treatment.MethodsEighty‐nine patients with HNC receiving radiotherapy were enrolled in the study. Patients were examined before radiotherapy and at 6 and 12 months after radiotherapy to evaluate the function and tenderness of the temporomandibular jaw and the muscles of mastication as well as the patient‐reported symptoms.ResultsAt 6 months after radiotherapy, there was a large increase in the number of patients reporting problems with opening their mouth, fatigue, stiffness, and pain of the jaw. Sixty‐eight percent of the patients had symptoms of TMD before oncological treatment and 94% and 81% had symptoms at the 6‐ and 12‐month follow‐up, respectively.ConclusionThe signs and symptoms of TMD escalate after radiotherapy, with symptoms peaking at 6 months after radiotherapy. Most commonly, patients suffer from restricted mouth opening, stiffness, fatigue, and pain of the jaw.
Thermal slip and radiative heat transfer effects on electro‐osmotic magnetonanoliquid peristaltic propulsion through a microchannel
Authors: Prakash J. , Siva E. P. , Tripathi D. , Bég O. Anwar 
Published: July 2019

AbstractA mathematical study is described to examine the concurrent influence of thermal radiation and thermal wall slip on the dissipative magnetohydrodynamic electro‐osmotic peristaltic propulsion of a viscous nanoliquid in an asymmetric microchannel under the action of an axial electric field and transverse magnetic field. Convective boundary conditions are incorporated in the model and the case of forced convection is studied, that is, thermal and species (nanoparticle volume fraction) buoyancy forces neglected. The heat source and sink effects are also included and the diffusion flux approximation is employed for radiative heat transfer. The transport model comprises the continuity, momentum, energy, nanoparticle volume fraction, and electric potential equations with appropriate boundary conditions. These are simplified by negating the inertial forces and invoking the Debye–Hückel linearization. The resulting governing equations are reduced into a system of nondimensional simultaneous ordinary differential equations, which are solved analytically. Numerical evaluation is conducted with symbolic software (MATLAB). The impact of different control parameters (Hartmann number, electro‐osmosis parameter, slip parameter, Helmholtz–Smoluchowski velocity, Biot numbers, Brinkman number, thermal radiation, and Prandtl number) on the heat, mass, and momentum characteristics (velocity, temperature, Nusselt number, etc) are presented graphically. Increasing Brinkman number is found to elevate temperature magnitudes. For positive Helmholtz–Smoluchowski velocity (reverse axial electrical field) temperature is strongly reduced, whereas for negative Helmholtz–Smoluchowski velocity (aligned axial electrical field), it is significantly elevated. With increasing thermal slip, nanoparticle volume fraction is also increased. Heat source elevates temperatures, whereas heat sink depresses them, across the microchannel span. Conversely, heat sink elevates nanoparticle volume fraction, whereas heat source decreases it. Increasing Hartmann (magnetic) parameter and Prandtl number enhance the nanoparticle volume fraction. Furthermore, with increasing radiation parameter, the Nusselt number is reduced at the extremities of the microchannel, whereas it is elevated at intermediate distances. The results reported provide a good insight into biomimetic energy systems exploiting electromagnetics and nanotechnology, and, furthermore, they furnish a useful benchmark for experimental and more advanced computational multiphysics simulations.
Viscosity and thermal conductivity comparative study for hybrid nanofluid in binary base fluids
Authors: Kumar Vikash , Sahoo Rashmi Rekha 
Published: July 2019

AbstractThermal conductivity and viscosity analysis of Al2O
3/CuO (50/50) hybrid nanofluid in various mass fractions of ethylene glycol (EG) and propylene glycol (PG) binary base fluid have been investigated in the present work. Hybrid nanofluid with vol. fraction range limited to 1.5% and within the higher temperature range of 50°C to 70°C is considered for thermal conductivity and viscosity analysis. Impact on viscosity and conductivity models with various shape nanoparticles, i.e, spherical, cylindrical, brick, platelets, and blades have been discussed and were compared in EG and PG binary base fluids. Also, the analysis extends to the prediction for the stability with zeta potential and synthesis of spherical shape Al2O3/CuO hybrid nanofluid with X‐ray diffraction (XRD) and scanning electron microscope (SEM). The theoretical analysis revealed that thermal conductivity of Al2O3/CuO hybrid nanofluid in EG binary base fluid is lower compared to in PG binary base fluid. The thermal conductivity is observed to be higher in spherical and cylindrical shape nanoparticle compared to bricks, blades, and platelets shape nanoparticles. Optimum viscosity of Al2O3/CuO hybrid nanofluid is observed at 50%EG and 30%PG of the binary base fluid. Hybrid nanofluid in 30% of PG as binary base fluid results 16.2% higher dynamic viscosity compared to pure PG base fluid for a volume concentration of 2%. Zeta potential measurement results in the stability of spherical Al2O3‐CuO/ (50/50) EG/W hybrid nanofluid, and it may be considered as a heat transfer fluid.
Epidemiological survey of antinuclear antibodies in healthy population and analysis of clinical characteristics of positive population
Authors: Li Xiaoyan , Liu Xin , Cui Jingjing , Song Wenjie , Liang Ying , Hu Yue , Guo Yaping 
Published: July 2019

AbstractBackgroundIn China, the incidence of autoimmune diseases is gradually increasing. To decrease the misdiagnosis rate of autoimmune diseases, we conducted an epidemiological investigation about the presence of antinuclear antibody (ANA) in healthy populations and analyzed the clinical characteristics of healthy population with both high titer of ANA and positive anti‐SSA and AMA‐M2.MethodsSerum ANA titers were detected by indirect immunofluorescence (IIF), and other 15 types of ANA‐specific antibodies were detected by line immunoassays.ResultsIn 25 110 individuals for routine examination, the positive rate of ANA titer >1:100 was 14.01%, of which the positive rate of female (19.05%) was higher than that of male (9.04%; P < 0.01). The positive rate of ANA titer >1:320 was 5.93%, of which the positive rate of female (8.68%) was higher than that of male (3.21%; P < 0.01). The specific antibodies were detected in 1489 of ANA‐positive people with titer >1:320, and the top three detected antibodies were anti‐Ro‐52 (212), AMA‐M2 (189), and anti‐SSA (144). The abnormal rate of blood routine test, liver function test, and other clinical indicators in AMA‐M2–positive population was significantly different from those in the control group. The abnormal rate of blood routine test, liver function test, and immune index in anti‐SSA–positive population was higher than those in control group.ConclusionThere was a high prevalence of ANA positive in healthy population. To avoid misdiagnosis, those who had symptoms of abdominal discomfort, pruritus, or fatigue with abnormal results of blood routine and liver function test should be examined for ANA, AMA‐M2, anti‐SSA as early as possible.
YAP1 is required for the angiogenesis in retinal microvascular endothelial cells via the inhibition of MALAT1‐mediated miR‐200b‐3p in high glucose‐induced diabetic retinopathy
Authors: Han Ning , Tian Wen , Yu Na , Yu Li 
Published: July 2019

AbstractDiabetic retinopathy (DR) is one of the severest complications in the development of diabetes with a characteristic of intraretinal new vessel formation. Our present study attempts to probe into the involvement of Yes‐associated protein 1 (YAP1) in retinal microvascular endothelial cells (RMECs) and the underlying mechanism. The DR mouse model was induced by streptozotocin injection and a high‐glucose/high‐fat diet. After that, with the utilization of the Pearson's correlation analysis, the correlation between YAP1 and metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) was analyzed. The relationship between microRNA‐200b‐3p (miR‐200b‐3p) and MALAT1 or vascular endothelial growth factor A (VEGFA) was then validated. Finally, the cellular processes including the abilities of proliferation, migration, as well as tube formation, were evaluated after the alteration of YAP1, MALAT1, and miR‐200b‐3p expression. YAP1 was observed to be highly expressed in retinas from DR mice, which promoted the cellular processes of RMECs through upregulating the MALAT1 expression. It was further confirmed that MALAT1 could sponge miR‐200b‐3p, and miR‐200b‐3p directly targeted VEGFA. When YAP1 was silenced, the RMEC proliferation, migration, and angiogenesis in the retina of DR mice were reduced. From these data, we conclude that YAP1 may exert some promotive effects on the development of DR through its regulation of the MALAT1/miR‐200b‐3p/VEGFA axis, highlighting that YAP1 silencing may be instrumental for the therapeutic targeting of DR.
Inhibition of KHSRP sensitizes colorectal cancer to 5‐fluoruracil through miR‐501‐5p‐mediated ERRFI1 mRNA degradation
Authors: Pan Ruijun , Cai Wei , Sun Jing , Yu Chaoran , Li Peiyong , Zheng Minhua 
Published: July 2019

AbstractK‐homology (KH)‐type splicing regulatory protein (KHSRP) is an RNA binding protein that participates in RNA variable splicing and stability, and facilitates the biogenesis of miRNAs that target mRNA. However, to date, the role of KHSRP in colorectal cancer (CRC) progression has not been reported. In this study, the function of KHSRP in CRC proliferation and 5‐fluoruracil (5‐FU) resistance was investigated. The upregulation of KHSRP expression was confirmed in CRC patient tissues and two CRC cell lines. Manipulating KHSRP expression altered cell proliferation and 5‐FU resistance in CRC cells. ERRFI1, a downstream effector of KHSRP in CRC cells, reduced CRC cell proliferation. Sensitivity to 5‐FU mediated by KHSRP knockdown was reversed by ERRFI1 knockdown. We found that KHSRP decreased ERRFI1 mRNA expression indirectly. By screening KHSRP‐regulated miRNAs, we further found that miR‐501‐5p directly combines with KHSRP in CRC cells. Mechanistically, the results of a luciferase assay suggested that miR‐501‐5p directly binds to the ERRFI1 3′‐untranslated region. Taken together, our data indicated that modification of ERRFI1 by KHSRP occurs through miR‐501‐5p, an essential mechanism driving CRC proliferation and 5‐FU resistance. Insight into this mechanism may provide novel targets for overcoming drug resistance in CRC.
Evaluation of the biocompatibility of resin composite‐based dental materials with gingival mesenchymal stromal cells
Authors: Cândea Ciurea Andreea , Şurlin Petra , Stratul Ştefan‐Ioan , Soancă Andrada , Roman Alexandra , Moldovan Mărioara , Tudoran B. Lucian , Pall Emoke 
Published: July 2019

AbstractResin composite‐based dental materials can leach certain components into the oral environment, causing potentially harmful gingival biological effect. Gingival tissue is a rich source of mesenchymal stem cells (MSCs) that is easily accessible, and can be used as a complementary approach for the investigation of dental material biocompatibility. Using gingival MSCs (gMSCs), the present study aimed to investigate the cytotoxicity of two classes of restorative dental materials (ormocers and resin composites) used to restore class II cavities close to the gingival margin, in addition to analyzing the leached compounds from these resin composite‐based materials. Functionality assays (Colony‐forming unit, migratory potential, and proliferation assays) and a viability assay (MTT) were employed. Cells' aspect was observed by scanning electron microscopy (SEM). Leached monomers were also quantitated using high‐performance liquid chromatography (HPLC). The cytotoxicity of the biomaterials was highlighted by impaired functionality and diminished viability of gMSCs. Despite being variants of the same commercial material, the two ormocers behaved differently one material having a more negative impact on cell functionality than the other. Cells appeared to attach well to all materials. Main monomer molecules were mostly released by the tested materials. For all samples, an increased elution of monomers was recorded in artificial saliva as compared with culture medium. One composite material has released nearly eight times more urethane dimetacrylate in artificial saliva than in culture medium. Significantly lower gMSC viability scores were recorded for all the investigated samples in comparison with the control.
Difficulties adapting to Nail‐Patella syndrome: A qualitative study of patients' perspectives
Authors: Geerts‐Crabbé Laura , Antoine Pascal , Brugallé Elodie , Ghoumid Jamal , Bellengier Laurence , Edery Patrick , Heron Delphine , Manouvrier‐Hanu Sylvie , Fantini‐Hauwel Carole 
Published: July 2019

AbstractNail‐Patella syndrome (NPS) is a genetic disorder generating physical malformations and, in approximately one in three cases, ocular and renal damage. The present research aimed to deeply understand patients' subjective experience with NPS, particularly the aspects of the syndrome that affect patients' adaptation and to propose interventions that can improve genetic and psychological counseling and help patients cope with their condition. Semi‐structured interviews of nine people diagnosed with NPS were analyzed using interpretative phenomenological analysis. Results highlighted attempts to look like a person without disabilities by hiding malformations and not telling the truth about symptoms' genetic origin because of patients' poor self‐esteem, negative self‐cognition, and social isolation experienced from childhood to adulthood. Difficulties of adaptation to physical limits and pain were also identified. The majority of participants who were not diagnosed at birth tended to consider physical symptoms as “birth malformations” without imagining other potential implications until receiving a diagnosis. Despite the diagnosis, the majority continued to minimize the potential complications by considering NPS as a “physical difference” and not adhering to medical surveillance.
Frontline Science: Abnormalities in the gut mucosa of non-obese diabetic mice precede the onset of type 1 diabetes
Authors: Miranda Mariana Camila Gonçalves , Oliveira Rafael Pires , Torres Lícia , Aguiar Sarah Leão Fiorini , Pinheiro-Rosa Natalia , Lemos Luísa , Guimarães Mauro Andrade , Reis Daniela , Silveira Tatiany , Ferreira Ênio , Moreira Thaís Garcias , Cara Denise Carmona , Maioli Tatiani Uceli , Kelsall Brian L , Carlos Daniela , Faria Ana Maria Caetano 
Published: July 2019

Abstract
Alterations in the composition of the intestinal microbiota have been associated with development of type 1 diabetes (T1D), but little is known about changes in intestinal homeostasis that contribute to disease pathogenesis. Here, we analyzed oral tolerance induction, components of the intestinal barrier, fecal microbiota, and immune cell phenotypes in non-obese diabetic (NOD) mice during disease progression compared to non-obese diabetes resistant (NOR) mice. NOD mice failed to develop oral tolerance and had defective protective/regulatory mechanisms in the intestinal mucosa, including decreased numbers of goblet cells, diminished mucus production, and lower levels of total and bacteria-bound secretory IgA, as well as an altered IEL profile. These disturbances correlated with bacteria translocation to the pancreatic lymph node possibly contributing to T1D onset. The composition of the fecal microbiota was altered in pre-diabetic NOD mice, and cross-fostering of NOD mice by NOR mothers corrected their defect in mucus production, indicating a role for NOD microbiota in gut barrier dysfunction. NOD mice had a reduction of CD103+ dendritic cells (DCs) in the MLNs, together with an increase of effector Th17 cells and ILC3, as well as a decrease of Th2 cells, ILC2, and Treg cells in the small intestine. Importantly, most of these gut alterations precede the onset of insulitis. Disorders in the intestinal mucosa of NOD mice can potentially interfere with the development of T1D due the close relationship between the gut and the pancreas. Understanding these early alterations is important for the design of novel therapeutic strategies for T1D prevention.
Sensitivity of Kupffer cells and liver sinusoidal endothelial cells to ricin toxin and ricin toxin–Ab complexes
Authors: Mooney Bridget , Torres-Velez Fernando J , Doering Jennifer , Ehrbar Dylan J , Mantis Nicholas J 
Published: July 2019

Abstract
Ricin toxin is a plant-derived, ribosome-inactivating protein that is rapidly cleared from circulation by Kupffer cells (KCs) and liver sinusoidal endothelial cells (LSECs)—with fatal consequences. Rather than being inactivated, ricin evades normal degradative pathways and kills both KCs and LSECs with remarkable efficiency. Uptake of ricin by these 2 specialized cell types in the liver occurs by 2 parallel routes: a “lactose-sensitive” pathway mediated by ricin's galactose/N-acetylgalactosamine-specific lectin subunit (RTB), and a “mannose-sensitive” pathway mediated by the mannose receptor (MR; CD206) or other C-type lectins capable of recognizing the mannose-side chains displayed on ricin's A (RTA) and B subunits. In this report, we investigated the capacity of a collection of ricin-specific mouse MAb and camelid single-domain (VHH) antibodies to protect KCs and LSECs from ricin-induced killing. In the case of KCs, individual MAbs against RTA or RTB afforded near complete protection against ricin in ex vivo and in vivo challenge studies. In contrast, individual MAbs or VHHs afforded little (&lt;40%) or even no protection to LSECs against ricin-induced death. Complete protection of LSECs was only achieved with MAb or VHH cocktails, with the most effective mixtures targeting RTA and RTB simultaneously. Although the exact mechanisms of protection of LSECs remain unknown, evidence indicates that the Ab cocktails exert their effects on the mannose-sensitive uptake pathway without the need for Fcγ receptor involvement. In addition to advancing our understanding of how toxins and small immune complexes are processed by KCs and LSECs, our study has important implications for the development of Ab-based therapies designed to prevent or treat ricin exposure should the toxin be weaponized.
Malt1 deficient mice develop osteoporosis independent of osteoclast-intrinsic effects of Malt1 deficiency
Authors: Monajemi Mahdis , Fisk Shera , Pang Yvonne C F , Leung Jessica , Menzies Susan C , Ben-Othman Rym , Cai Bing , Kollmann Tobias R , Rozmus Jacob , Sly Laura M 
Published: July 2019

Abstract
This study tested the hypothesis that mucosa associated lymphoid tissue 1 (Malt1) deficiency causes osteoporosis in mice by increasing osteoclastogenesis and osteoclast activity. A patient with combined immunodeficiency (CID) caused by MALT1 deficiency had low bone mineral density resulting in multiple low impact fractures that was corrected by hematopoietic stem cell transplant (HSCT). We have reported that Malt1 deficient Mϕs, another myeloid cell type, are hyper-responsive to inflammatory stimuli. Our objectives were to determine whether Malt1 deficient mice develop an osteoporosis-like phenotype and whether it was caused by Malt1 deficiency in osteoclasts. We found that Malt1 deficient mice had low bone volume by 12 weeks of age, which was primarily associated with reduced trabecular bone. Malt1 protein is expressed and active in osteoclasts and is induced by receptor activator of NF-κB ligand (RANKL) in preosteoclasts. Malt1 deficiency did not impact osteoclast differentiation or activity in vitro. However, Malt1 deficient (Malt1−/−) mice had more osteoclasts in vivo and had lower levels of serum osteoprotegerin (OPG), an endogenous inhibitor of osteoclastogenesis. Inhibition of Malt1 activity in Mϕs induced MCSF production, required for osteoclastogenesis, and decreased OPG production in response to inflammatory stimuli. In vitro, MCSF increased and OPG inhibited osteoclastogenesis, but effects were not enhanced in Malt1 deficient osteoclasts. These data support the hypothesis that Malt1 deficient mice develop an osteoporotic phenotype with increased osteoclastogenesis in vivo, but suggest that this is caused by inflammation rather than an effect of Malt1 deficiency in osteoclasts.
Isolation of aristolochic acid I from herbal plant using molecular imprinted polymer composited ionic liquid‐based zeolitic imidazolate framework‐67
Authors: Fang Luwei , Tian Minglei , Row Kyung Ho , Yan Xuemin , Xiao Wei 
Published: July 2019

AbstractAristolochic acid I is a toxic compound found in the genus of Aristolochia plants, which are commonly used as herbal cough treatment medicines. To remove the aristolochic acid I in extract efficiently and selectively, a molecularly imprinted polymer composed of ethylimidazole ionic liquid‐based zeolitic imidazolate framework‐67 was synthesized and used as the adsorbent. Under the conditions optimized by the software design expert, the sorbent showed highest adsorption amount of 34.25 mg/g in methanol/water (95:5, v/v) at 39°C for 138 min. The sorbent was then applied to solid phase extraction to isolate aristolochic acid I from the extract of the herbal plant Fibraurea Recisa Pierre. 0.043 mg/g of aristolochic acid I was obtained after the loading, washing, and elution processes. The limit of detection of 2.41 × 10−5 mg/mL and good recoveries provided evidence for the accuracy of this method.