bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brainstem catecholaminergic neurones and breathing control during postnatal development in male and female rats
Authors: Patrone Luis Gustavo A. , Biancardi Vivian , Marques Danuzia A. , Bícego Kênia C. , Gargaglioni Luciane H. 
Published: February 2018

Key points
The brainstem catecholaminergic (CA) modulation on ventilation changes with development.
We determined the role of the brainstem CA system in ventilatory control under normocapnic and hypercapnic conditions during different phases of development [postnatal day (P)7–8, P14–15 and P20–21] in male and female Wistar rats.
Brainstem CA neurones produce a tonic inhibitory drive that affects breathing frequency in P7–8 rats and provide an inhibitory drive during hypercapnic conditions in both males and females at P7–8 and P14–15.
In pre‐pubertal rats, brainstem CA neurones become excitatory for the CO2 ventilatory response in males but remain inhibitory in females.
Diseases such as sudden infant death syndrome, congenital central hypoventilation syndrome and Rett syndrome have been associated with abnormalities in the functioning of CA neurones; therefore, the results of the present study contribute to a better understanding of this system.
AbstractThe respiratory network undergoes significant development during the postnatal phase, including the maturation of the catecholaminergic (CA) system. However, postnatal development of this network and its effect on the control of pulmonary ventilation () is not fully understood. We investigated the involvement of brainstem CA neurones in respiratory control during postnatal development [postnatal day (P)7–8, P14–15 and P20–21], in male and female rats, through chemical injury with conjugated saporin anti‐dopamine β‐hydroxylase (DβH‐SAP). Thus, DβH‐SAP (420 ng μL−1), saporin (SAP) or phosphate buffered solution (PBS) was injected into the fourth ventricle of neonatal Wistar rats of both sexes. and oxygen consumption were recorded 1 week after the injections in unanaesthetized neonatal and juvenile rats during room air and hypercapnia. The resting ventilation was higher in both male and female P7–8 lesioned rats by 33%, with a decrease in respiratory variability being observed in males. The hypercapnic ventilatory response (HCVR) was altered in male and female lesioned rats at all postnatal ages. At P7–8, the HCVR for males and females was increased by 37% and 30%, respectively. For both sexes at P14–15 rats, the increase in during hypercapnia was 37% higher for lesioned rats. A sex‐specific difference in HCRV was observed at P20–21, with lesioned males showing a 33% decrease, and lesioned females showing an increase of 33%. We conclude that brainstem CA neurones exert a tonic inhibitory effect on in the early postnatal days of the life of a rat, increase variability in P7–8 males and modulate HCRV during the postnatal phase.
Invasion of the Brain by
Listeria monocytogenes
Is Mediated by InlF and Host Cell Vimentin
Authors: Ghosh Pallab , Halvorsen Elizabeth M. , Ammendolia Dustin A. , Mor-Vaknin Nirit , O’Riordan Mary X. D. , Brumell John H. , Markovitz David M. , Higgins Darren E. 
Published: February 2018

ABSTRACT

Listeria monocytogenes
is a facultative intracellular bacterial pathogen that is frequently associated with food-borne infection. Of particular concern is the ability of
L. monocytogenes
to breach the blood-brain barrier, leading to life-threatening meningitis and encephalitis. The mechanisms used by bacterial pathogens to infect the brain are not fully understood. Here we show that
L. monocytogenes
is able to utilize vimentin for invasion of host cells. Vimentin is a type III intermediate filament protein within the cytosol but is also expressed on the host cell surface. We found that
L. monocytogenes
interaction with surface-localized vimentin promoted bacterial uptake. Furthermore, in the absence of vimentin,
L. monocytogenes
colonization of the brain was severely compromised in mice. The
L. monocytogenes
virulence factor InlF was found to bind vimentin and was necessary for optimal bacterial colonization of the brain. These studies reveal a novel receptor-ligand interaction that enhances infection of the brain by
L. monocytogenes
and highlights the importance of surface vimentin in host-pathogen interactions.

IMPORTANCE
Listeria monocytogenes
is an intracellular bacterial pathogen that is capable of invading numerous host cells during infection.
L. monocytogenes
can cross the blood-brain barrier, leading to life-threatening meningitis. Here we show that an
L. monocytogenes
surface protein, InlF, is necessary for optimal colonization of the brain in mice. Furthermore, in the absence of vimentin, a cytosolic intermediate filament protein that is also present on the surface of brain endothelial cells, colonization of the brain was significantly impaired. We further show that InlF binds vimentin to mediate invasion of host cells. This work identifies InlF as a bacterial surface protein with specific relevance for infection of the brain and underscores the significance of host cell surface vimentin interactions in microbial pathogenesis.
Immunohistochemical evaluation of stress-responsive protein sestrin2 and its correlation with p53 mutational status in eyelid sebaceous gland carcinoma
Authors: Jayaraj Perumal , Sen Seema , Rangarajan Srishti , Ray Neelanjana , Vasu Kirtana , Singh Vijay Kumar , Phartyal Rajendra , Yadav Sarika , Verma Anita 
Published: February 2018

Backgroundp53 is a stress-activated tumour suppressor gene, and its mutation has been associated with solid tumours including non-melanoma skin cancers. Sestrin2 expression is associated with DNA damage and oxidative stress and has been described as a downstream target of p53 network. However, its role in sebaceous gland carcinoma (SGC) remains unexplored.ObjectivesTo determine the role of p53 and its downstream target gene sestrin2 expression and p53 gene mutation status in SGC.MethodsTwenty cases of eyelid SGC tumour and circulating cell-free DNA (ccfDNA) were subjected to mutational analysis of p53 gene. p53 and sesrin2 expression was evaluated by immunohistochemistry. Results were correlated with the clinicopathological features of eyelid SGC.Resultsp53 gene mutations was detected in 25% of the SGC cases. A C>T transition was identified in exon 6 in a single patient in both tumour and ccfDNA. A G>T transversion leading to amino acid change D259Y was seen in four patients. A splice site mutation affected a single case in exon 6. p53 expression was observed in 55% SGC. Loss of sestrin2 in 55% SGC cases correlated with poor tumour differentiation (P=0.0001), upper eyelid involvement (P=0.004), p53 mutation (P=0.039) and with mutant p53 expression (P=0.0001).ConclusionSestrin2 expression was found to be significantly reduced in p53 mutated SGC cases and in cases with strong p53 nuclear immunopositivity, suggesting that loss of sestrin2 may be of biological significance in the development of SGC and as a key downstream component of p53 tumour suppression network in eyelid SGC.
Insulin analogues use in pregnancy among women with pregestational diabetes mellitus and risk of congenital anomaly: a retrospective population-based cohort study
Authors: Wang Hao , Wender-Ozegowska Ewa , Garne Ester , Morgan Margery , Loane Maria , Morris Joan K , Bakker Marian K , Gatt Miriam , de Walle Hermien , Jordan Susan , Materna-Kiryluk Anna , Nelen Vera , Thys Guy , Wiesel Awi , Dolk Helen , de Jong-van den Berg Lolkje T W 
Published: February 2018

ObjectivesTo evaluate the risk of major congenital anomaly associated with first-trimester exposure to insulin analogues compared with human insulin in offspring of women with pregestational diabetes.Design and settingA population-based cohort of women with pregestational diabetes (n=1661) who delivered between 1996 and 2012 was established retrospectively from seven European regions covered bythe European Surveillance of Congenital Anomalies (EUROCAT) congenital anomaly registries.Primary outcome measuresThe risk of non-chromosomal major congenital anomaly in live births, fetal deaths and terminations for a fetal anomaly exposed to insulin analogues in the first trimester of pregnancy was compared with the risk in those exposed to human insulin only.ResultsDuring the first trimester, 870 fetuses (52.4%) were exposed to human insulin only, 397 fetuses (23.9%) to insulin analogues only and 394 fetuses (23.7%) to both human insulin and insulin analogues. The risk of major congenital anomaly in fetuses exposed to insulin analogues only was lower than those exposed to human insulin only; the relative risk adjusted for glycaemic control and region was 0.56 (95% CI 0.29 to 1.06). The significantly lower risk related to exposure of insulin analogues only was observed in congenital heart defects: adjusted relative risk 0.14 (95% CI 0.03 to 0.62).ConclusionsIn this retrospective population-based cohort study across Europe, first-trimester exposure to insulin analogues did not increase the risk of major congenital anomaly compared with exposure to human insulin. A possible lower risk of congenital heart defects among fetuses exposed to insulin analogues only deserves further investigation.
Evaluation of Mediterranean diet adherence scores: a systematic review
Authors: Zaragoza-Martí A , Cabañero-Martínez MJ , Hurtado-Sánchez JA , Laguna-Pérez A , Ferrer-Cascales R 
Published: February 2018

ObjectiveThe aim of this review was to evaluate the conceptual suitability, applicability and psychometric properties of scores used internationally to measure adherence to the Mediterranean diet (MD).DesignThis was a systematic review to identify original articles that examined some aspects of the conceptual suitability, applicability or psychometric properties of the MD adherence score. Electronic searches were carried out on the international databases MEDLINE, Scopus, Web of Science and EMBASE (from January 1980 to 31 December 2015).Eligibility criteria for selecting studiesThe study included original articles that examined some aspects of the conceptual suitability, applicability or psychometric properties of the MD adherence score. The studies where MD adherence scores were administered but did not bring forward any evidence about their performance related to conceptual suitability, applicability or psychometric properties were excluded.Data extractionInformation relating to the scales was extracted in accordance with the quality criteria defined by the Scientific Advisory Committee of the Medical Outcomes Trust for measurement of health results and the quality criteria recommended by Terwee: (1) conceptual, (2) applicability and (3) psychometric properties. Three authors independently extracted information from eligible studies.ResultsTwenty-seven studies were identified as meeting the inclusion criteria, yielding 28 MD adherence scores. The results showed that evidence is scarce and that very few scores fulfilled the applicability parameters and psychometric quality. The scores developed by Panagiotakoset al, Bucklandet aland Sotos-Prietoet alshowed the highest levels of evidence.ConclusionsScores measuring adherence to MD are useful tools for identifying the dietary patterns of a given population. However, further information is required regarding existing scores. In addition, new instruments with greater conceptual and methodological rigour should be developed and evaluated for their psychometric properties.
Targeting strategies of mHealth interventions for maternal health in low and middle-income countries: a systematic review protocol
Authors: Ilozumba Onaedo , Abejirinde Ibukun-Oluwa Omolade , Dieleman Marjolein , Bardají Azucena , Broerse Jacqueline E W , Van Belle Sara 
Published: February 2018

IntroductionRecently, there has been a steady increase in mobile health (mHealth) interventions aimed at improving maternal health of women in low-income and middle-income countries. While there is evidence indicating that these interventions contribute to improvements in maternal health outcomes, other studies indicate inconclusive results. This uncertainty has raised additional questions, one of which pertains to the role of targeting strategies in implementing mHealth interventions and the focus on pregnant women and health workers as target groups. This review aims to assess who is targeted in different mHealth interventions and the importance of targeting strategies in maternal mHealth interventions.Methods and analysisWe will search for peer-reviewed, English-language literature published between 1999 and July 2017 in PubMed, Web of Knowledge (Science Direct, EMBASE) and Cochrane Central Registers of Controlled Trials. The study scope is defined by the Population, Intervention, Comparison and Outcomes framework: P, community members with maternal or reproductive needs; I, electronic health or mHealth programmes geared at improving maternal or reproductive health; C, other non-electronic health or mHealth-based interventions; O, maternal health measures including family planning, antenatal care attendance, health facility delivery and postnatal care attendance.Ethics and disseminationThis study is a review of already published or publicly available data and needs no ethical approval. Review results will be published in a peer-reviewed journal and presented at international conferences.PROSPERO registration numberCRD42017072280.
A Novel ReRAM-Based Processing-in-Memory Architecture for Graph Traversal
Authors: Han Lei , Shen Zhaoyan , Liu Duo , Shao Zili , Huang H. Howie , Li Tao 
Published: February 2018

Graph algorithms such as graph traversal have been gaining ever-increasing importance in the era of big data. However, graph processing on traditional architectures issues many random and irregular memory accesses, leading to a huge number of data movements and the consumption of very large amounts of energy. To minimize the waste of memory bandwidth, we investigate utilizing processing-in-memory (PIM), combined with non-volatile metal-oxide resistive random access memory (ReRAM), to improve both computation and I/O performance.
We propose a new ReRAM-based processing-in-memory architecture called RPBFS, in which graph data can be persistently stored and processed in place. We study the problem of graph traversal, and we design an efficient graph traversal algorithm in RPBFS. Benefiting from low data movement overhead and high bank-level parallel computation, RPBFS shows a significant performance improvement compared with both the CPU-based and the GPU-based BFS implementations. On a suite of real-world graphs, our architecture yields a speedup in graph traversal performance of up to 33.8×, and achieves a reduction in energy over conventional systems of up to 142.8×.
A Soft Microenvironment Protects from Failure of Midbody Abscission and Multinucleation Downstream of the EMT-Promoting Transcription Factor Snail
Authors: Simi Allison K. , Anlaş Alişya A. , Stallings-Mann Melody , Zhang Sherry , Hsia Tiffaney , Cichon Magdalena , Radisky Derek C. , Nelson Celeste M. 
Published: February 2018

Abstract
Multinucleation is found in more than one third of tumors and is linked to increased tolerance for mutation, resistance to chemotherapy, and invasive potential. The integrity of the genome depends on proper execution of the cell cycle, which can be altered through mechanotransduction pathways as the tumor microenvironment stiffens during tumorigenesis. Here, we show that signaling downstream of matrix metalloproteinase-3 (MMP3) or TGFβ, known inducers of epithelial–mesenchymal transition (EMT), also promotes multinucleation in stiff microenvironments through Snail-dependent expression of the filament-forming protein septin-6, resulting in midbody persistence, abscission failure, and multinucleation. Consistently, we observed elevated expression of Snail and septin-6 as well as multinucleation in a human patient sample of metaplastic carcinoma of the breast, a rare classification characterized by deposition of collagen fibers and active EMT. In contrast, a soft microenvironment protected mammary epithelial cells from becoming multinucleated by preventing Snail-induced upregulation of septin-6. Our data suggest that tissue stiffening during tumorigenesis synergizes with oncogenic signaling to promote genomic abnormalities that drive cancer progression.
Significance: These findings reveal tissue stiffening during tumorigenesis synergizes with oncogenic signaling to promote genomic abnormalities that drive cancer progression. Cancer Res; 78(9); 2277–89. ©2018 AACR.
<b>Conserving the newly recorded Hill Arecanut Palm <I>Bentinckia condapanna</I> Berry ex Roxb (Arecaceae) population outside the natural forest as wildlife corridor</b>
Authors: Murukesh M. Divin , Ashokan Ajith 
Published: February 2018

Bentinckia condapanna a vulnerable palm distributed along the southern Western Ghats has a sparse distribution in Kerala and Tamil Nadu. A viable population of this palm is found in Arundel-Priya Estate, an abandoned tea plantation in Kollam District, Kerala. This population comprises 76 mature palms and is on the verge of being affected with the proposed widening of Kazhuthurutty-Arundel-Achankoil road and may wipe out the whole population.
<b>Two new reports of thrips (Thysanoptera: Thripidae) from India</b>
Authors: Rachana R. R. , Varatharajan R. 
Published: February 2018

Caliothrips punctipennis (Hood) and the male of Western Flower Thrips Frankliniella occidentalis (Pergande) have been recorded for the first time from India. F. occidentalis was collected on the leaves of Erythrina indica from Ooty in the Nilgiris, the Western Ghats, southern India, whereas C. punctipennis was collected from yellow pan traps laid at Great Nicobar, India. Considering the quarantine importance of the pest F. occidentalis, the report of the male for the first time in India needs attention and concern. Males are also known to be more effective vectors of tospoviruses than females. Xerochrysum bracteatum, the Common Golden Everlasting Daisy, is often taken out of Ooty by tourists to other parts of the country. This along with other planting materials carried by tourists and farmers could aid in the dispersal of F. occidentalis to the temperate regions of northern and southern India, where it is likely to thrive in the congenial climatic conditions prevalent there. Under these circumstances, it is imperative that quarantine mechanisms within the country are activated and strengthened, to prevent the spread of this notorious pest to the rest of India from the pockets of its occurrence in southern India - particularly the Nilgiris. The diagnostic characters of both species are discussed.
<b>Rapid assessment of sacred groves: a biodiversity assessment tool for ground level practitioners</b>
Authors: Trivedi Shivam , Bharucha Erach , Mungikar Rahul 
Published: February 2018

Sacred groves in the Western Ghats are culturally preserved patches of forests that are rich in diversity owing to protection by several generations of local people, providing excellent examples of community based conservation. Sacred groves harbour local populations, preserve genetic resources and serve as reference sites and corridors between protected areas. They are considered to be cornerstones of biodiversity conservation, but are insufficient in scale and number to significantly address many aspects of the management of landscapes and biodiversity. We studied 13 sacred groves in Pune District that are at present outside the protected areas of the northern Western Ghats, where we employed a simple and rapid biodiversity assessment technique that can be replicated by frontline foresters, local residents and Biodiversity Management Committees (BMCs). Integrating these sacred sites into the conservation network through local support can improve the efficiency for the existing protected area network in this ecologically fragile region.
<b>Hunted species and hunting equipment used by rainforest poachers in Ghana</b>
Authors: Wiafe Edward Debrah 
Published: February 2018

This study investigated species that are hunted in protected rainforest areas and the methods used to hunt them, using data obtained by recording items in the possession of 17 poachers arrested in Kakum Conservation Area in Ghana over a period of 12 months. Twelve species were recorded among 69 total animals. Most were mammals, including primates. Shotguns and wire snares were the main hunting methods used. Primates must be given special protection and conservation attention, as they were found to be prominent among the animals poached.
Children’s Control/Display Stereotypes
Authors: Hoffmann Errol R. , Chan Alan H. S. , Tai Judy P. C. 
Published: February 2018

Objective The aim of this study was to determine control/display stereotypes for children of a range of ages and development of these stereotypes with age. Background Little is known about control/display stereotypes for children of different ages and the way in which these stereotypes develop with age. This study is part of a program to determine the need to design differentially for these age groups. Method We tested four groups of children with various tasks (age groups 5 to 7, 8 to 10, 11 to 13, 14 to 16), with about 30 in each group. Examples of common tasks were opening a bottle, turning on taps, and allocating numbers to keypads. More complex tasks involved rotating a control to move a display in a requested direction. Results Tasks with which different age groups were familiar showed no effect of age group. Different control/display arrangements generally showed an increase in stereotype strength with age, with dependence on the form of the control/display arrangement. Two-dimensional arrangements, with the control on the same plane as the display, had higher stereotype strength than three-dimensional arrangements for all age groups, suggesting an effect of familiarity with controls and displays with increasing age. Conclusion Children’s control/display stereotypes do not differ greatly from those of adults, and hence, design for children older than 5 years of age, for control/display stereotypes, can be the same as that for adult populations. Application When designing devices for children, the relationship between controls and displays can be as for adult populations, for which there are considerable experimental data.