bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sarcasm as Pedagogy of Love: Exploring Ironic Speech Acts in an Urban High School English Classroom
Authors: Larson Joanne , Morris Timothy , Shaw Kristen 
Published: March 2019

AbstractThe authors explore how the use of sarcasm in an urban high school English classroom fostered critical language awareness and positive relationships among diverse classroom participants. The guiding research question was, What were the social‐pedagogical functions of sarcasm in this classroom? Drawing on interactional sociolinguistics and pragmatics, the authors found that sarcasm was used to construct a sense of belonging, which supported building trusting relationships and complex language learning.
A pharmacy resident‐driven pneumococcal vaccination protocol increases vaccination rates in hospitalized patients over 65 years
Authors: Wells Corey , Monte Scott V. , Prescott William A. , Danek Melissa , Gargala Emma , Woodruff Ashley E. 
Published: March 2019

IntroductionPneumococcal disease remains a leading cause of serious illness in adults aged 65 years and older. Despite this, pneumococcal vaccination rates remain suboptimal in this population.ObjectivesTo implement a pharmacy resident‐driven pneumococcal vaccination protocol and to assess vaccination rates and the return on investment (ROI) for patients age 65 years and older admitted to an internal medicine service at an academic medical center.MethodsA retrospective, single center review of a newly implemented pharmacy resident‐driven pneumococcal vaccination protocol targeting patients age 65 years and older admitted to an internal medicine service was conducted. The primary outcome was the percentage of eligible patients, as defined by the Center for Disease Control vaccination guidelines, who received a pneumococcal vaccine prior to discharge over a 60‐day period. The secondary outcome was ROI from the hospital perspective, which was calculated as follows: net profit (defined as total revenue—total cost) divided by total cost and then multiplied by 100. Pneumococcal vaccination rates and ROI data were presented descriptively.ResultsA total of 84 patients were included in the pharmacy resident‐driven protocol, 37 (42%) of whom were up‐to‐date on their pneumococcal vaccinations. Among the remaining 51 vaccine‐eligible patients, 33 (64.7%) were vaccinated prior to hospital discharge: 19/33 (57.6%) received the pneumococcal conjugate vaccine (PCV13) vaccine and 14/33 (42.4%) received the pneumococcal polysaccharide vaccine (PPSV23) vaccine. The total revenue and cost generated by the protocol over the 60‐day period was $8276.10 and $6406.88, respectively. The ROI for the protocol was calculated to be 29.2%.ConclusionThe implementation of a pharmacy resident‐driven pneumococcal vaccination protocol increased pneumococcal vaccination rates while also serving as a revenue source. Health care systems may consider implementing a similar protocol to increase vaccination rates and provide novel revenue generating models for their institution.
Estimating the U.S. output gap with state‐level data
Authors: González‐Astudillo Manuel 
Published: March 2019

SummaryThis paper develops a method to estimate the U.S. output gap by exploiting the cross‐sectional variation of state‐level output and unemployment rate data. The model assumes that there are common output and unemployment rate trend and cycle components, and that each state's output and unemployment rate are subject to idiosyncratic trend and cycle perturbations. I estimate the model with Bayesian methods using quarterly data from 2005:Q1 to 2018:Q2 for the 50 states and the District of Columbia. Results show that the U.S. output gap reached about negative 4.6% around the years of the Great Recession and was about 0.9% in 2018:Q2.
Cr(VI)‐induced genotoxicity and cell cycle arrest in human osteoblast cell line MG‐63
Authors: Monteiro Cristina , Santos Conceição , Bastos Verónica , Oliveira Helena 
Published: March 2019

AbstractOccupational environments are major exposure routes to Cr(VI). However, Cr(VI) may also establish in bone tissues by ingestion or through Cr containing orthopaedic prostheses that, due to wear and corrosion, may release metal particles and ions potentially affecting bone tissue. The aim of this work was to evaluate the effects of clinically relevant concentrations of Cr(VI) in human osteoblasts, by integrating genotoxic effects, evaluated by the comet assay and cytokinesis‐blocked micronucleus assay (scoring the presence of micronucleus, nucleoplasmic bridges and nuclear division index), with the effects on cell cycle and cell viability. Human osteoblasts MG‐63 were in vitro exposed to Cr(VI) at concentrations ranging from 0.1 to 5 μm, for 24 and 48 hours. Results pointed out to a decrease of cell viability for both time exposures in a time‐ and dose‐dependent manner, which was related to cell cycle arrest and DNA damage. Chromosome abnormalities were also observed. Hence, these data suggest that cells arrested in the cell division with DNA damage may have followed cell death pathways, while some surviving ones still revealed DNA damage at chromosome level indicating abnormal cell division progression. In conclusion, Cr(VI) induced cytotoxic and genotoxic effects in human bone cells at concentrations that could be found in patients with metal‐on‐metal prostheses. In addition, the early onset of genotoxic damage induced by Cr(VI) at low concentrations after 24 hours of cell exposure alert to the relevance of periodic monitoring of patients for genotoxicity diagnosis after implantation of prostheses before clinical symptoms appear.
Estrogen receptor genes polymorphisms determine serum lipid profile in healthy postmenopausal women treated with calcium, vitamin D, and genistein
Authors: Usategui‐Martín Ricardo , Pérez‐Alonso María , Socorro‐Briongos Laisa , Ruiz‐Mambrilla Marta , De Luis Daniel , Linares Lidia , Calero‐Paniagua Ismael , Dueñas‐Laita Antonio , Pérez‐Castrillón José L. 
Published: March 2019

AbstractCardiovascular risk increases in women after menopause. Unfavorable lipid‐lipoprotein changes due to a lack of estrogens may have an important role in this context. Estrogen actions are mainly mediated by their binding to two estrogen receptors (ERs) whose signaling may be conditioned by different factors. Calcium, vitamin D, and genistein, among others, cause a beneficial effect on serum lipid profile by its modulation. Some genetic factors can also determine this signal. We determined the possible additive effect of genistein on calcium and vitamin D supplementation regarding serum lipid profile changes and whether ER polymorphisms may mediate in this effect.We performed a prospective, double blind study in which women were randomized in two groups: one group received calcium and vitamin D and the other group received calcium, vitamin D and genistein. Subsequently, we studied rs9340799, rs928554, and rs4986938 ER polymorphisms in both groups.Our results showed that being a carrier of the variant allele G of rs928554 polymorphism was associated with a greater decrease in triglyceride levels and that the homozygous AA genotype of rs9340799 polymorphism was associated with a greater decrease in total cholesterol, low‐density lipoprotein cholesterol, and triglyceride levels after calcium, vitamin D, and genistein supplementation.This is the first report showing an association between polymorphisms in ER genes and an improvement of the serum lipid profile after taking calcium, vitamin D, and genistein supplementation in postmenopausal women. It reinforces the hypothesis that genetic factors are crucial in ER signalling.
miRNA‐145‐5p induces apoptosis after ischemia‐reperfusion by targeting dual specificity phosphatase 6
Authors: Wu Gang , Tan Jiaying , Li Junping , Sun Xiaoli , Du Lei , Tao Sun 
Published: March 2019

AbstractDisorders mainly caused by ischemia‐reperfusion (I/R), including stroke and myocardial infarction, is linked to debilitating health conditions and death. Recent research indicates that microRNAs (miRNAs) mediate the process of ischemic pathology. This study investigated the effects of miR‐145‐5p in regulating myocardial ischemic injury. The I/R models were established in rat cardiomyocytes H9C2 and rats. Western blot analysis and quantitative polymerase chain reaction was performed to analyze protein expression. Annexin V‐FITC/PI staining was conducted to evaluate cell apoptosis. The application of miR‐145‐5p mimics and inhibitor revealed that miR‐145‐5p promoted apoptosis in cardiomyocytes. Furthermore, we found that miR‐145‐5p directly inhibited dual specificity phosphatase 6 (DUSP6) by luciferase reporter assay. The results indicated that DUSP6 was beneficial against I/R injury through inhibiting c‐Jun N‐terminal kinase pathways. In conclusion, the essential roles of miR‐145‐5p and DUSP6 in I/R provide a novel therapeutic target to develop future intervention strategies.
MicroRNA signature of air pollution exposure‐induced congenital defects
Authors: Li Zheng , Ma Jianqing , Bi Jiaqi , Guo Haiwei , Chan Matthew T. V. , Wu William K. K. , Wu Zhanyong , Shen Jianxiong 
Published: March 2019

AbstractAir pollution exposure has been increasing extensively and there are evidence suggesting that exposure to air pollution during pregnancy can lead to congenital defects in the offspring. Recent findings suggested that microRNAs (miRNAs) might play important roles in the pathogenesis of developmental defects. However, the miRNAs profile pattern in the air pollution‐exposed embryos remains unknown. RNA sequencing was performed to determine the differentially expressed miRNAs in the rat embryos (gestation day 9) with or without air pollution exposure. The potential functions and the associated mechanisms of these differentially expressed miRNAs were determined using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) analyses. The regulatory networks of mRNA–miRNA interactions were also reconstructed. As compared with the control group, a total of 291 miRNAs were differentially expressed in the rat embryos from the air pollution‐exposed group, in which 204 and 87 miRNAs were significantly downregulated and upregulated, respectively. These miRNAs were predicted to deregulate mitotic spindle organization, cellular respiration, glycolate metabolism, and proteasome. Extensive regulation of target genes by miR‐346, miR‐504, miR‐214‐3p and miR‐1224 was also predicted. Our results suggested that miRNAs may play crucial roles in the pathogenesis of air pollution‐induced congenital spinal defects through deregulating multiple biological processes.
Targeted delivery of tacrolimus to T cells by pH‐responsive aptamer‐chitosan‐ poly(lactic‐co‐glycolic acid) nanocomplex
Authors: Mansouri Atena , Abnous Khalil , Alibolandi Mona , Taghdisi Seyed Mohammad , Ramezani Mohammad 
Published: March 2019

AbstractTacrolimus (TAC) acts as an inhibitor of calcineurin, which inhibits the production of interleukin‐2. In this study, we aimed to design a targeted delivery platform with poly (lactide‐co‐glycolide; PLGA) nanoparticles modified with chitosan (CS) and CD8AP17s aptamer (Apt). MOLT‐4 cells as CD8 positive and JURKAT cells as CD negative were adopted to investigate the efficacy of the proposed delivery system in vitro. The particle size and Ζ potential of the TAC‐PLGA‐CS‐Apt nanocomplex were 345 nm and 13.7 mV, respectively. Release study showed an efficient TAC release from complex in citrate buffer (pH 5.5). The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay showed that TAC‐PLGA‐CS‐Apt nanocomplex was highly selective toward MOLT‐4 cells. Complex increased the cellular uptake of TAC in MOLT‐4 cells (target) while reducing its cytotoxicity in JURKAT cells (nontarget). Our study showed that complex nanoconjugate could efficiently deliver TAC into MOLT‐4 cells as a model of cytotoxic T cell and it could be considered as a potential candidate for TAC delivery.
LncRNA‐MEG3 promotes bovine myoblast differentiation by sponging miR‐135
Authors: Liu Mei , Li Bo , Peng Wenwen , Ma Yilei , Huang Yongzhen , Lan Xianyong , Lei Chuzhao , Qi Xinglei , Liu George E. , Chen Hong 
Published: March 2019

AbstractLong noncoding RNA maternally expressed gene 3 (lncRNA‐MEG3) is an important regulator in multiple biological functions. However,
lncRNA‐MEG3's function in cattle growth and regulatory mechanism on bovine skeletal muscle development has not yet been well studied. In this project, we first investigated
lncRNA‐MEG3's expression profile and detected that it was highly expressed in bovine skeletal muscle tissue and its RNA level was kept increasingly during the early phase of bovine primary myoblast differentiation. Using luciferase reporter assays, we identified the
lncRNA‐MEG3 core promoter containing putative transcription factor binding site for myocyte enhancer factor 2C (MEF2C). Interestingly, we found that
LncRNA‐MEG3 could significantly upregulate and downregulate myosin heavy chain (
MHC), myogenin (
MyoG), and
MEF2C through overexpression and RNAi strategies, respectively. Using luciferase reporter assays, we also verified
lncRNA‐MEG3 as a miR‐135 sponge. Overexpression of miR‐135 markedly inhibited the wild type of
lncRNA‐MEG3, but not the mutant
lncRNA‐MEG3 reporter. The luciferase activity of miR‐135 sensor could be rescued by
lncRNA‐MEG3 via competing for miRNA‐135. In addition, the luciferase activity of
MEF2C was significantly upregulated by the wild type of
lncRNA‐MEG3. This study, for the first time, revealed that
lncRNA‐MEG3 could promote bovine skeletal muscle differentiation via interacting with miRNA‐135 and
MEF2C. The results were valuable for further studies and applications of lncRNA related roles in beef molecular breeding.
Velocity vector imaging echocardiography and NT‐proBNP study of fetal cardiac function in pregnancy‐induced maternal hypertension
Authors: Yu Li , Zhou Qichang , Peng Qinghai , Zeng Shi , Yang Zurong 
Published: March 2019

AbstractPurposeTo investigate whether acute and transient pressure overload in patients with pregnancy‐induced hypertension affects cardiac function in fetuses.MethodsWe enrolled 104 singleton pregnant women with gestational ages ranging 30 to 33 weeks, visiting for prenatal care. Among them, 34 had gestational hypertension (GH), 32 had preeclampsia (PE), and 38 were healthy and formed the control group. Conventional echocardiographic and velocity vector imaging (VVI) variables were prospectively collected. Blood levels of N‐terminal pro‐brain natriuretic peptide (NT‐proBNP) were measured from cord blood drawn at birth.ResultsThe fetuses of mothers with preeclampsia had significantly lower left (LV) and right ventricle (RV) diastolic strain rate (SRd) and RV strain (S) and systolic strain rate (SRs) than controls. LV and RV S, SRd, and SRS were not different in fetuses of mothers with GH and controls. The NT‐proBNP levels were higher in fetuses of patients with PE than in GH and controls.ConclusionsIn the third trimester of pregnancy, fetal biventricular function and NT‐proBNP levels are not significantly influenced by GH. Fetuses of mothers with PE present signs of LV and RV diastolic dysfunction, right ventricular systolic dysfunction, and elevated NT‐pro‐BNP levels. VVI echocardiography appears more sensitive than conventional echocardiography to evaluate fetal cardiac function.
MRI of acute appendicitis
Authors: Mervak Benjamin M. , Wilson Sarah B. , Handly Brian D. , Altun Ersan , Burke Lauren M. 
Published: March 2019

Appendicitis is the most common cause of acute abdominal pain resulting in surgery. While historically ultrasound (US) and computed tomography (CT) have been used to evaluate for appendicitis and its related complications, magnetic resonance imaging (MRI) has become a highly accurate and increasingly utilized modality in the last two decades, particularly in the pediatric and pregnant patient populations in whom ionizing radiation is used reluctantly. This article discusses the advantages and disadvantages of MRI as a modality to evaluate for acute appendicitis, summarizes studies of the diagnostic performance relative to CT and US, provides a standard MR protocol, and describes MRI findings typical of acute appendicitis, common complications, and other differential diagnoses.Level of Evidence: 2Technical Efficacy Stage: 2J. Magn. Reson. Imaging 2019;50:1367–1376.
Theoretical chemical ionization rate constants of the concurrent reactions of hydronium ions (H3O+) and oxygen ions (O) with selected organic iodides
Authors: Strekowski Rafal  S. , Alvarez Coralie , Petrov‐Stojanović Jeanne , Hagebaum‐Reignier Denis , Wortham Henri 
Published: March 2019

AbstractShort chain volatile iodinated organic compounds (VIOCs) are of great importance in many fields that include atmospheric chemistry, agriculture, and environmental chemistry related to nuclear power plant safety. Proton‐transfer‐reaction mass spectrometry (PTR‐MS) allows for fast, sensitive, and online quantification of VIOCs if the chemical ionization (CI) reaction rate coefficients are known. In this work, the theoretical CI rate coefficients for the reactions of hydronium ions (H3O+) and oxygen ions (O
) with selected atmospherically important short chain VIOCs are determined. The neutral CH3I, CH2I2, C2H5I, iso‐C3H7I, n‐C3H7I, n‐C4H9I, 2‐C4H9I, n‐C5H11I, 2‐C5H11I, and 3‐C5H11I have been chosen because these compounds are of atmospheric and environmental importance in the field of safety of nuclear plant reactors. Theoretical ion‐molecule collision rate coefficients were determined using the Su and Chesnavich theory based on parametrized trajectory calculations. The proton affinity, ionization energy, dipole moment, and polarizability values of the neutral molecules were determined from density functional theory and coupled‐cluster calculations. The newly calculated rate constants facilitate the use of the CI mass spectrometry in the atmospheric quantification of selected VIOCs.
Asymptomatic hepatitis B carriers who were vaccinated at birth
Authors: Wang Xueyan , Chen Qinyan , Li Hai , Wang Chao , Hu Liping , Yang Qingli , Ren Chuangchuang , Liu Huabing , Zheng Zhigang , Harrison Tim J. , Fang Zhong‐Liao 
Published: March 2019

AbstractThe long‐term persistence of immunity following universal infant immunization against hepatitis B virus (HBV) and the need for a subsequent booster dose in adolescence remain under debate. With data derived from Long'an County, Guangxi, China, we reported previously that the prevalence of hepatitis B surface antigen (HBsAg) among adults born from 1987 to 1993 increases with age, although these individuals had received a first dose of the vaccine within 24 hours of birth. Here, we sought the source of transmission by comparison of genotypes among their family members using phylogenetic analysis of complete HBV S gene sequences. For comparison, we screened 2199 vaccinated individuals aged 5 to 17 in Cang Wu County and 1592 vaccinated individuals aged 3 to 7 in Ling Shan County in Guangxi for HBsAg carriers and investigate their family members. In total, 50 asymptomatic HBsAg carriers who were vaccinated at birth and 152 family members were analyzed. The results showed that 25% (95% CI: 6.0‐44.0) of the HBsAg‐positive children had not acquired their HBV infection from their mothers. This phenomenon showed a trend that increases with age. Antibody escape mutations were detected in 22.9% (95% CI: 11.0‐34.8) of the isolates. In conclusion, a booster dose may be necessary for adolescence who were vaccinated at birth in highly endemic countries.
Understanding and Prediction of the Clouding Phenomenon by Spontaneous and Effective Packing Concepts
Authors: Pleines Maximilian , Kunz Werner , Zemb Thomas 
Published: March 2019

AbstractIn this work, we show by which mechanism branching of the hydrocarbon chains influences the cloud point of nonionic, ethoxylated surfactants. The temperature‐induced separation into a dilute and a concentrated liquid phase is of liquid–gas type and can be explained by the relative probability of endcaps and branching points of the cylindrical micelles in both phases. The influence of branches on the hydrocarbon chains can be easily understood by means of spontaneous and effective packing concepts, while quantification via hydrophilic/lipophilic balance (HLB), hydrophilic‐lipophilic difference (HLD), and HLD‐Net Average Curvature requires parametrization. The phase equilibrium above the cloud point phenomenon is equivalent to the Winsor I type phase equilibrium, one of the five known phase equilibria in ternary systems.
Microflora of fresh white button mushrooms (Agaricus bisporus) during cold storage revealed by high‐throughput sequencing and MALDI‐TOF mass spectrometry fingerprinting
Authors: Qiu Wanwei , Huang Ying , Zhao Chao , Lin Zhenshan , Lin Wenxing , Wang Zejin 
Published: March 2019

ABSTRACTBACKGROUNDFresh Agaricus bisporus is popular and consumed throughout the world because of its taste, as well as its nutritional and medicinal properties, but it is also prone to microbial growth. There is very limited information about the dynamic changes of microbial communities during storage. The present study aimed to analyze the microbial diversity of button mushroom during cold storage using Illumina HiSeq sequencing. Bacteria isolated from the later storage period were identified by MALDI‐TOF mass spectrometry and a bioassay of pathogenicity was carried out.RESULTSHigh‐throughput sequencing showed that Pseudomonas was the predominant genus throughout the storage period. Pedobacter and Flavobacterium grew prolifically on the eighth day, while the relative abundance of Oscillospira continued to decrease. Pseudomonas, Ewingella and Chryseobacterium were isolated at the later period of mushroom storage. A pathogenicity bioassay on the cap of mushrooms showing brown blotch indicated an infection by Pseudomonas tolaasii. However, Ewingella americana did not have a pathogenic effect in our study.CONCLUSIONBacterial communities of fresh Agaricus bisporus during cold storage were characterized by high‐throughput sequencing. MALDI‐TOF MS provides a good analytical procedure, in addition to 16S rRNA gene sequencing. © 2019 Society of Chemical Industry
Multi‐analytical characterization of perigonadal fat in bluefin tuna: from waste to marine lipid source
Authors: Saliu Francesco , Magoni Chiara , Lasagni Marina , Della Pergola Roberto , Labra Massimo 
Published: March 2019

AbstractBACKGROUNDMarine lipids are widely recognized as supplements beneficial to health in the human diet. Bluefin tuna, as most migrating fish, may have high and variable marine lipid contents in their tissues. The viscera of the specimens caught in the Mediterranean Sea are generally discarded. Only ovaries are (locally) used for artisanal seafood preparations.RESULTSA multi‐analytical approach carried out using infrared (IR), nuclear magnetic resonance (NMR) and mass spectrometry (MS) revealed that the perigonadal fats usually discarded during bluefin tuna evisceration are characterized by a high relative abundance of polyunsaturated triacylglycerols (76.2% of dry mass), with docosahexaenoic (DHA, 22:6) and eicosapentaenoic (EPA, 20:5) acids preferentially located at the sn‐2 position of the glycerol backbone. By contrast, in fats from the ovarian tissues, no wax esters and phospholipids were evidenced. Cholesterol was found in very low amounts (84 mg/100 g ovarian tissue). In vitro assays showed that this fatty material is rapidly hydrolyzed by lipase and is prone to auto‐oxidation, especially if compared to the stable wax esters contained in the ovary.CONCLUSIONThe results show that the perigonadal fats of bluefin tuna, traditionally wasted after evisceration, may be an interesting source for the preparation of marine lipid supplements that do not require complex fractionation processes. © 2019 Society of Chemical Industry