bluuebunny/crossref_metadata_2025_split huggingface dataset:
GATA3 germline variant is associated with CRLF2 expression and predicts outcome in pediatric B‐cell precursor acute lymphoblastic leukemia
Authors: Madzio Joanna , Pastorczak Agata , Sedek Lukasz , Braun Marcin , Taha Joanna , Wypyszczak Kamila , Trelinska Joanna , Lejman Monika , Muszynska‐Roslan Katarzyna , Tomasik Bartlomiej , Derwich Katarzyna , Koltan Andrzej , Kazanowska Bernarda , Irga‐Jaworska Nina , Badowska Wanda , Matysiak Michal , Kowalczyk Jerzy , Styczynski Jan , Fendler Wojciech , Szczepanski Tomasz , Mlynarski Wojciech 
Published: March 2019

AbstractThe germline variant at rs3824662 in GATA3 is a risk locus for Philadelphia‐like acute lymphoblastic leukemia (Ph‐like ALL), the biological subtype of B‐cell precursor (BCP)‐ALL defined by a distinct gene expression profile and the presence of specific somatic aberrations including rearrangements of CRLF2. In this study, we investigated whether rs3824662 in GATA3 associates with CRLF2 expression in leukemic cells and predicts prognosis in pediatric BCP‐ALL patients treated according to the ALL Intercontinental Berlin‐Frankfurt‐Münster (IC BFM) 2009 (n = 645) and the ALL IC BFM 2002 (n = 216) protocols. High expression of CRLF2 was observed at both protein and mRNA levels (fourfold higher in AA than in CA + CC) among GATA3 AA variant carriers, independent of the presence of P2RY8‐CRLF2 fusion. Additionally, the AA variant at rs3824662 was a significant factor affecting minimal residual disease level at the end of induction phase and overall survival regardless of the risk group and the protocol. The germline variant at rs3824662 in GATA3 is a prognostic factor which associates with CRLF2 expression in leukemic cells supporting the hypothesis that GATA3 may have a regulatory effect on the CRLF2 pathway in pediatric BCP‐ALL.
Resting‐state fMRI detects the effects of learning in short term: A visual search training study
Authors: Bueichekú Elisenda , Miró‐Padilla Anna , Ávila César 
Published: March 2019

AbstractCan resting‐state functional connectivity (rs‐FC) detect the impact of learning on the brain in the short term? To test this possibility, we have combined task‐FC and rs‐FC tested before and after a 30‐min visual search training. Forty‐two healthy adults (20 men) divided into no‐contact control and trained groups completed the study. We studied the connectivity between four different regions of the brain involved in visual search: the primary visual area, the right posterior parietal cortex (rPPC), the right dorsolateral prefrontal cortex (rDLPFC), and the dorsal anterior cingulate cortex (dACC). Task‐FC showed increased connectivity between the rPPC and rDLPFC and between the dACC and rDLPFC from pretraining to posttraining for both the control group and the trained group, suggesting that connectivity between these areas increased with task repetition. In rs‐FC, we found enhanced connectivity between these regions in the trained group after training, especially in those with better learning. Whole brain independent component analyses did not reveal any change in main networks after training. These results imply that rs‐FC may not only predict individual differences in task performance, but rs‐FC might also serve to monitor the impact of learning on the brain after short periods of cognitive training, localizing them in brain areas specifically involved in training.
Tumor‐induced hypophosphatemic osteomalacia caused by a mesenchymal tumor of the mandible managed by a segmental mandibulectomy and microvascular reconstruction with a free fibula flap
Authors: Acharya Rishabh P. , Won Alexander M. , Moon Bryan S. , Flint James H. , Roubaud Margaret S. , Williams Michelle D. , Hessel Amy C. , Murphy William A. , Chambers Mark S. , Gagel Robert F. 
Published: March 2019

AbstractBackgroundTumor‐induced osteomalacia is a rare paraneoplastic syndrome in which patients develop hypophosphatemia and osteomalacia.Methods and ResultsHere, we report a unique case of a 42‐year‐old man who presented to our institution with a 1‐year history of pain in his ribs, hips, lower back, and feet. Radiologic examination revealed a decrease in bone density and multiple insufficiency fractures. Laboratory evaluation revealed hypophosphatemia, low serum 1,25 dihydroxy vitamin D3, and elevated fibroblast growth factor 23 (FGF23). A positron emission tomography/CT scan showed increased uptake in the right mandibular third molar region. Panoramic radiography and CT scanning showed a lytic expansile bone lesion. A mandibular bone biopsy revealed a mixed connective tissue tumor. A right segmental mandibulectomy was performed, followed by microvascular reconstruction. The resection was confirmed by normalization of serum phosphate and FGF23.ConclusionSuccessful management of this condition was achieved, with complete surgical resection of the tumor and reconstructive surgery.
Collagen Formation Assessed by N‐Terminal Propeptide of Type 3 Procollagen Is a Heritable Trait and Is Associated With Liver Fibrosis Assessed by Magnetic Resonance Elastography
Authors: Caussy Cyrielle , Bhargava Meera , Villesen Ida F. , Gudmann Natasja S. , Leeming Diana J. , Karsdal Morten A. , Faulkner Claire , Bao Denny , Liu Amy , Lo Min‐Tzu , Bettencourt Ricki , Bassirian Shirin , Richards Lisa , Brenner David A. , Chen Chi‐Hua , Sirlin Claude B. , Loomba Rohit 
Published: March 2019

N‐terminal propeptide of type 3 procollagen (PRO‐C3) is a biomarker of liver fibrosis in nonalcoholic fatty liver disease (NAFLD). This study examines the association between PRO‐C3 concentration and liver fibrosis assessed by magnetic resonance elastography (MRE)–measured stiffness (MRE‐stiffness) and the heritability of PRO‐C3 concentration in a cohort of twins and families with and without NAFLD. We performed a cross‐sectional analysis of a well‐characterized prospective cohort of 306 participants, including 44 probands with NAFLD‐cirrhosis and their 72 first‐degree relatives, 24 probands with NAFLD without advanced fibrosis and their 24 first‐degree relatives, and 72 controls without NAFLD and their 72 first‐degree relatives. Liver steatosis was assessed by magnetic resonance imaging proton density fat fraction, and liver fibrosis was assessed by MRE‐stiffness. Serum PRO‐C3 was assessed by competitive, enzyme‐linked immunosorbent assay. We assessed the familial correlation of PRO‐C3 concentration, the shared gene effects between PRO‐C3 concentration and liver steatosis and fibrosis, and the association between PRO‐C3 concentration and genetic variants in the patatin‐like phospholipase domain–containing 3 (PNPLA3), transmembrane 6 superfamily member 2 (TM6SF2), membrane‐bound O‐acyltransferase domain–containing (MBOAT), and glucokinase regulator (CGKR) genes. In multivariable‐adjusted models including age, sex, body mass index, and ethnicity, serum PRO‐C3 correlated strongly with liver fibrosis (r
2 = 0.50, P < 0.001) and demonstrated robust heritability (h
2, 0.36; 95% confidence interval [CI], 0.07, 0.59; P = 0.016). PRO‐C3 concentration and steatosis had a strong genetic correlation (shared genetic determination: 0.62; 95% CI, 0.236, 1.001; P = 0.002), whereas PRO‐C3 concentration and fibrosis had a strong environmental correlation (shared environmental determination: 0.55; 95% CI, 0.317, 0.717; P < 0.001). PRO‐C3 concentrations were higher in carriers of the TM6SF2 rs58542926‐T allele compared with noncarriers: 15.7 (± 10.5) versus 10.8 (± 5.7) ng/L (P = 0.047). Conclusion: Serum PRO‐C3 correlates with MRE‐assessed fibrosis, is heritable, shares genetic correlation with liver steatosis and shares environmental correlation with liver fibrosis. PRO‐C3 concentration appears to be linked to both fibrosis and steatosis and increased in carriers of the TM6SF2 rs58542926 risk allele.
A Behavioral Health Program for Alcohol Use Disorder, Substance Abuse, and Depression in Chronic Liver Disease
Authors: Verma Manisha , Horrow Jay , Navarro Victor 
Published: March 2019

Alcohol use disorder, substance abuse, and depression are illnesses that deteriorate the quality of life (QOL) of patients with chronic liver disease (CLD). Screening and behavioral health programs integrated into routine practice can mitigate the deleterious effects of such illnesses but have not been adopted in hepatology practices. We implemented a behavioral health program based on the Screening, Brief Intervention, and Referral to Treatment (SBIRT) model and assessed its acceptability and effectiveness in improving QOL. This was a quality improvement study. Patients with CLD and a scheduled outpatient visit in the hepatology clinic were screened while waiting for their appointment. All patients who screened positive for any of the three illnesses were offered a brief intervention (BI) at the point of care and at 3 months by a trained social worker. The BI used the principles of motivational interviewing and cognitive behavioral therapy. Severity of illness was assessed at baseline, 3 months, and 6 months. Participants completed an acceptability survey at 6 months. We screened 303 patients; 61.7% were positive for any of the three illnesses assessed. Among the positive patients, depression was most common (48.4%), alcohol and substance abuse were each 26%. For the 95 patients who underwent BI, QOL improved from baseline to 3 and 6 months (P < 0.001) and patients with depression improved the most. Depression was the only independent predictor of change in QOL over time. Of the enrolled patients, 82% agreed BIs improved their overall care and 87% indicated a desire to continue with the behavioral program. Conclusion: An outpatient behavioral health program based on the SBIRT model is acceptable to patients with CLD and may help improve QOL over time.
Status of public–private partnership recognition and willingness to pay for private health care in China
Authors: Wang Yongfei , Zhang Liang 
Published: March 2019

SummaryObjectivesThe Chinese government has been increasingly engaging and interacting with the private sectors to initiate public–private partnerships (PPPs) to enhance the capacity of the health care system. Thus, the availability of private health services and copays are increasing. However, Chinese residents appear to be more accustomed to public health care because it has long been the dominant health service provider in China. Therefore, learning how the Chinese recognize PPPs, whether they would like to use private health services and how much they are willing to pay out of pocket are important for policymakers.MethodsA bibliographical search was performed through PubMed in Medline, the Chinese National Knowledge Infrastructure and key government websites using a combination of keywords from seminal papers on PPPs and then sequentially added “willingness to pay (WTP), health, and private.” The papers selected were closely related to PPPs and WTP for health care in China, and then, over 100 peer‐reviewed literature, opinion, or commentary papers and postgraduate theses on the particular topics were carefully reviewed.ResultsOut of 139 papers closely related to WTP for private health care, 37 were extensively reviewed and substantially analyzed. The results corroborate that Chinese residents are generally willing to pay for high‐risk disease screening, improved therapies, and health insurance, although the WTP intentions and the amounts they are willing to pay are different among distinct populations for the various types of health services. The results also present conditions that the responders are not explicitly willing to pay for or are willing to pay additional fees for their health promotion. Education level, family per capita income, self‐reported health status, and dwelling districts (such as urban or rural residents) are the main factors that affect cost‐sharing intention.ConclusionsPPPs are increasingly recognized as an effective means to mobilize resources in the private sector to relieve the pressure/burden placed on the public system during medical reform in China. Responders in China are generally willing to pay reasonable fees out of pocket for primary health care services or for improved therapies; however, cost‐sharing intentions are affected by multiple factors. Health‐related policymakers should learn the general health concerns/demands of the residents and their WTP status to perfect the design of PPPs for the benefit of the majority population in China.
Natural history of human papillomavirus and vaccinations in men: A literature review
Authors: Lieblong Benjamin J. , Montgomery Brooke E. E. , Su L. Joseph , Nakagawa Mayumi 
Published: March 2019

AbstractBackground and aimsInfection with high‐risk (HR) genotypes of the human papillomavirus (HPV) is necessary for and causative of almost all cervical cancers and their precursor condition, cervical intraepithelial neoplasia. These conditions have been sharply reduced by cervical cytology screening, and a further decrease is expected because of the recent introduction of prophylactic HPV vaccinations. While significant attention has been given to gynecologic HPV disease, men can be affected by HPV‐related cancers of the anus, penis, and oropharynx. This literature review aims to address disparities in HPV‐related disease in men, and certain HR male subpopulations, compared with women.DiscussionOverall, immunocompetent men are far less likely than women to develop anogenital HPV‐related cancers, despite harboring HR HPV infections at anogenital sites. On the other hand, men who have sex with men and men living with human immunodeficiency virus infection are at considerably higher risk of HPV‐related disease. Historic rates of prophylactic HPV vaccination in males have trailed those of females due to numerous multilevel factors, although, in recent years, this sex gap in vaccination coverage has been closing. In the absence of routine HPV screening in males, therapeutic vaccinations have emerged as a potential treatment modality for preinvasive neoplasia and are in various phases of clinical testing.ConclusionSuccessful reductions in HPV disease morbidity at the population level must acknowledge and target HPV infections in men.
Spatial controls on the distribution and dynamics of a marginal snowpack in the Australian Alps
Authors: Bilish Shane P. , Callow J. Nikolaus , McGrath Gavan S. , McGowan Hamish A. 
Published: March 2019

AbstractSeasonal snowpacks in marginal snow environments are typically warm and nearly isothermal, exhibiting high inter‐ and intra‐annual variability. Measurements of snow depth and snow water equivalent were made across a small subalpine catchment in the Australian Alps over two snow seasons in order to investigate the extent and implications of snowpack spatial variability in this marginal setting. The distribution and dynamics of the snowpack were found to be influenced by upwind terrain, vegetation, solar radiation, and slope. The role of upwind vegetation was quantified using a novel parameter based on gridded vegetation height. The elevation range of the catchment was relatively modest (185 m), and elevation impacted distribution but not dynamics. Two characteristic features of marginal snowpack behaviour are presented. Firstly, the evolution of the snowpack is described in terms of a relatively unstable accumulation state and a highly stable ablation state, as revealed by temporal variations in the mean and standard deviation of snow water equivalent. Secondly, the validity of partitioning the snow season into distinct accumulation and ablation phases is shown to be compromised in such a setting. Snow at the most marginal locations may undergo complete melt several times during a season and, even where snow cover is more persistent, ablation processes begin to have an effect on the distribution of the snowpack early in the season. Our results are consistent with previous research showing that individual point measurements are unable to fully represent the variability in the snowpack across a catchment, and we show that recognising and addressing this variability are particularly important for studies in marginal snow environments.
Successful treatment of ureteral stricture after total hysterectomy: An antegrade ureteroscopic approach facilitates the insertion of a guidewire for endoscopic dilation
Authors: Iwamoto Genta , Kawahara Takashi , Takeshima Teppei , Ninomiya Sahoko , Takamoto Daiji , Mochizuki Taku , Kuroda Shinnosuke , Yao Masahiro , Uemura Hiroji 
Published: March 2019

IntroductionIatrogenic ureteral injury is sometimes seen in daily clinical practice, and gynecological surgery carries the highest risk of ureteral injury among iatrogenic surgical ureteral injury.Case presentationA 61‐year‐old woman was referred to our department for right ureteral stricture and hydronephrosis after total hysterectomy. We initially attempted ureteral stenting, but hydronephrosis redeveloped 1 month after ureteral stent removal. We performed ureteroscopy via an antegrade approach and successfully incised and dilated the ureter.ConclusionWe encountered a case of severe ureteral stenosis after total hysterectomy that was successfully treated endoscopically using ureteroscopy via an antegrade approach.
SWI post processing using granularity controlled edge‐preserved denoising of multichannel GRE images
Authors: Madhusoodhanan Sreekanth , Kesavadas Chandrasekharan , Paul Joseph S. 
Published: March 2019

AbstractThe influence of granularities in the background suppressed phase of susceptibility‐weighted images (SWI) and susceptibility‐weighted angiogram (SWAN) becomes significant when the susceptibility based contrast is enhanced by exponential weighting of the high‐pass filtered phase. Furthermore, the effect of noise due to the inherently low signal‐to‐noise ratio resulting from high‐resolution SWI/SWAN acquisition, can be minimized by application of edge‐preserved denoising of the channel phase images without loss of venous structural details. Simultaneous reduction of granularity effects with edge‐preserved denoising is achieved using the proposed granularity controlled adaptive edge‐preserved regularization (GRADER). In this approach, the edge‐preserving cost is minimized with respect to the desired channel phase image and an unknown scale parameter that adaptively tunes the high‐pass filter. The algorithm is implemented using quasi‐Newton type iterations, with the scale parameter updated using a search procedure in each alternating minimization step. The iterations are stopped once the scale parameter converges to a steady state value. Extension of GRADER to parallel MRI (pMRI) by processing the real and imaginary components of complex channel images (IR‐GRADER) results in enhanced susceptibility‐related contrast‐to‐noise ratio of the magnitude SWI, leading to improved visualization of superficial veins and deep gray matter structures.
Photopolymerizable gelatin and hyaluronic acid for stereolithographic 3D bioprinting of tissue‐engineered cartilage
Authors: Lam Tobias , Dehne Tilo , Krüger Jan Philipp , Hondke Sylvia , Endres Michaela , Thomas Alexander , Lauster Roland , Sittinger Michael , Kloke Lutz 
Published: March 2019

AbstractTo create artificial cartilage in vitro, mimicking the function of native extracellular matrix (ECM) and morphological cartilage‐like shape is essential. The interplay of cell patterning and matrix concentration has high impact on the phenotype and viability of the printed cells. To advance the capabilities of cartilage bioprinting, we investigated different ECMs to create an in vitro chondrocyte niche. Therefore, we used methacrylated gelatin (GelMA) and methacrylated hyaluronic acid (HAMA) in a stereolithographic bioprinting approach. Both materials have been shown to support cartilage ECM formation and recovery of chondrocyte phenotype. We used these materials as bioinks to create cartilage models with varying chondrocyte densities. The models maintained shape, viability, and homogenous cell distribution over 14 days in culture. Chondrogenic differentiation was demonstrated by cartilage‐typical proteoglycan and type II collagen deposition and gene expression (COL2A1, ACAN) after 14 days of culture. The differentiation pattern was influenced by cell density. A high cell density print (25 × 106 cells/mL) led to enhanced cartilage‐typical zonal segmentation compared to cultures with lower cell density (5 × 106 cells/mL). Compared to HAMA, GelMA resulted in a higher expression of COL1A1, typical for a more premature chondrocyte phenotype. Both bioinks are feasible for printing in vitro cartilage with varying differentiation patterns and ECM organization depending on starting cell density and chosen bioink. The presented technique could find application in the creation of cartilage models and in the treatment of articular cartilage defects using autologous material and adjusting the bioprinted constructs size and shape to the patient. © 2019 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2649–2657, 2019.
Biodegradable hyaluronan hydrogel coatings on acellular dermis grafts—A potential strategy to improve biologic graft durability in hernia repair application
Authors: Sahoo Sambit , Ma Jinjin , Tastaldi Luciano , Baker Andrew R. , Loftis Jacki , Rosen Michael J. , Derwin Kathleen A. 
Published: March 2019

AbstractBiologic grafts used in hernia repair undergo rapid cellular infiltration and remodeling, but their premature degradation often results in hernia recurrence. We hypothesize that a temporary barrier that prevents infiltration of acute inflammatory cells into the graft during the initial 4 weeks of implantation could mitigate graft degradation. The purpose of this study is to design tyramine‐substituted hyaluronan (THA) hydrogel coatings with tunable degradation properties, as a means to develop a resorbable barrier for human acellular dermis grafts (HADM). THA plugs prepared at different cross‐linking densities, by varying cross‐linking agent concentration (0.0001–0.0075% H2O2), demonstrated varying rates ofin vitrodegradation (25 U/mL hyaluronidase, 48 h). Based on these results, HADM grafts were coated with THA at three cross‐linking densities (0.0001%, 0.00075%, and 0.003% H2O2) and THA coating degradation was evaluatedin vitro(25 U/mL hyaluronidase, 48 h) andin vivo(rat intraperitoneal implantation, 1–4 weeks). THA coatings degradedin vitroandin vivowith the lowest cross‐linking density (0.0001% H2O2), generally showing greater degradation as evidenced by significant decrease in coating cross‐sectional area. However, all three coatings remained partially degraded after 4 weeks ofin vivoimplantation. Alternate strategies to acceleratein vivodegradation of THA coatings are required to allow investigation of the study hypothesis. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2664–2672, 2019.