bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant Protein Production in Microalgae: Emerging Trends
Authors: Ahmad Niaz , Mehmood Muhammad Aamer , Malik Sana 
Published: October 2019

:
In recent years, microalgae have emerged as an alternative platform for large-scale
production of recombinant proteins for different commercial applications. As a production
platform, it has several advantages, including rapid growth, easily scale up and ability to grow with
or without the external carbon source. Genetic transformation of several species has been
established. Of these, Chlamydomonas reinhardtii has become significantly attractive for its
potential to express foreign proteins inexpensively. All its three genomes – nuclear, mitochondrial
and chloroplastic – have been sequenced. As a result, a wealth of information about its genetic
machinery, protein expression mechanism (transcription, translation and post-translational
modifications) is available. Over the years, various molecular tools have been developed for the
manipulation of all these genomes. Various studies show that the transformation of the chloroplast
genome has several advantages over nuclear transformation from the biopharming point of view.
According to a recent survey, over 100 recombinant proteins have been expressed in algal
chloroplasts. However, the expression levels achieved in the algal chloroplast genome are generally
lower compared to the chloroplasts of higher plants. Work is therefore needed to make the algal
chloroplast transformation commercially competitive. In this review, we discuss some examples
from the algal research, which could play their role in making algal chloroplast commercially
successful.
Expression of a Functional Recombinant Human Glycogen Debranching Enzyme (hGDE) in N. benthamiana Plants and in Hairy Root Cultures
Authors: Rodriguez-Hernandez Meilyn , Triggiani Doriana , Ivison Fiona , Demurtas Olivia C. , Illiano Elena , Marino Carmela , Franconi Rosella , Massa Silvia 
Published: October 2019

Background:Glycogen storage disease type III (GSDIII, Cori/Forbes disease) is a metabolic disorder due to the deficiency of the Glycogen Debranching Enzyme (GDE), a large monomeric protein (about 176 kDa) with two distinct enzymatic activities: 4-α-glucantransferase and amylo-α-1,6-glucosidase. Several mutations along the amylo-alpha-1,6-glucosidase,4-alphaglucanotransferase (Agl) gene are associated with loss of enzymatic activity. The unique treatment for GSDIII, at the moment, is based on diet. The potential of plants to manufacture exogenous engineered compounds for pharmaceutical purposes, from small to complex protein molecules such as vaccines, antibodies and other therapeutic/prophylactic entities, was shown by modern biotechnology through “Plant Molecular Farming”.Objective/method:In an attempt to develop novel protein-based therapeutics for GSDIII, the Agl gene, encoding for the human GDE (hGDE) was engineered for expression as a histidinetagged GDE protein both in Nicotiana benthamiana plants by a transient expression approach, and in axenic hairy root in vitro cultures (HR) from Lycopersicum esculentum and Beta vulgaris.Results:In both plant-based expression formats, the hGDE protein accumulated in the soluble fraction of extracts. The plant-derived protein was purified by affinity chromatography in native conditions showing glycogen debranching activity.Conclusion:These investigations will be useful for the design of a new generation of biopharmaceuticals based on recombinant GDE protein that might represent, in the future, a possible therapeutic option for GSDIII.
LipoSVM: Prediction of Lysine lipoylation in Proteins based on the Support Vector Machine
Authors: Wu Meiqi , Lu Pengchao , Yang Yingxi , Liu Liwen , Wang Hui , Xu Yan , Chu Jixun 
Published: October 2019

Background:Lysine lipoylation which is a rare and highly conserved post-translational modification of proteins has been considered as one of the most important processes in the biological field. To obtain a comprehensive understanding of regulatory mechanism of lysine lipoylation, the key is to identify lysine lipoylated sites. The experimental methods are expensive and laborious. Due to the high cost and complexity of experimental methods, it is urgent to develop computational ways to predict lipoylation sites.Methodology:In this work, a predictor named LipoSVM is developed to accurately predict lipoylation sites. To overcome the problem of an unbalanced sample, synthetic minority over-sampling technique (SMOTE) is utilized to balance negative and positive samples. Furthermore, different ratios of positive and negative samples are chosen as training sets.Results:By comparing five different encoding schemes and five classification algorithms, LipoSVM is constructed finally by using a training set with positive and negative sample ratio of 1:1, combining with position-specific scoring matrix and support vector machine. The best performance achieves an accuracy of 99.98% and AUC 0.9996 in 10-fold cross-validation. The AUC of independent test set reaches 0.9997, which demonstrates the robustness of LipoSVM. The analysis between lysine lipoylation and non-lipoylation fragments shows significant statistical differences.Conclusion:A good predictor for lysine lipoylation is built based on position-specific scoring matrix and support vector machine. Meanwhile, an online webserver LipoSVM can be freely downloaded from https://github.com/stars20180811/LipoSVM.
Selective Estrogen Receptor Modulators (SERMs): Mechanistic Insights Against Microbial Infections
Authors: Garg Aakriti , Singh Balraj , Sharma Ruchika , Singh Arti , Kumar Anoop 
Published: October 2019

Background:
Infections are one of the leading causes of death worldwide
and currently available treatments remain unsatisfactory due to rise in the cases of
antimicrobial resistance. Thus, there is a need for the development of new drugs with
different mechanisms of action. However, the development of new antimicrobials agents
is a long and expensive process. Hence, most of the pharmaceutical companies are

Methodology:
The data related to SERMs and microbial infection has been extracted
from Pub Med (from January 1997 to December 2018). A total of 101 studies have been
published from 1997 -2018 regarding SERMs and microbial infections.

Results:
On the basis of inclusion and exclusion criteria, 25 studies have been included
for the analysis of level of evidence regarding antimicrobial effects of SERMs. Emerging
reports have indicated the antimicrobial property of selective estrogen receptor
modulators (SERMs) against normal and resistant strains under in vitro and in vivo
conditions against wide variety of microorganisms through different mechanisms of
action.

Conclusion:
In conclusion, SERMs could be developed as a broad spectrum
antimicrobial agent alone or in combination with existing antimicrobial agents.
Antipyretic Plants: An Updated Review
Authors: Garg Vandana , Dutt Rohit 
Published: October 2019

Background:
Fever, is known as pyrexia, may occur due to infection, inflammation, or any
tissue damage and disease states. Normally, the infected or damaged tissue initiates the enhanced formation
of pro-inflammatory mediators like cytokines which further increases the synthesis of prostaglandin
E2 (PgE2) near the hypothalamic area and thereby trigger the hypothalamus to elevate the body temperature.

Objective:
Antipyretics are the agents which reduce the elevated body temperature. The most commonly
used antipyretic agent, paracetamol, may be fatal due to its side effects.

Methods:
In this review paper, Chemical Abstracts, Google Scholar, PubMed, and Science Direct were
the sources for the published article to collect information regarding antipyretic activity.

Results:
This review compiles the antipyretic plants that may be useful to treat fever due to various
diseases.

Conclusion:
These medicinal plants could be good alternatives for traditional allopathic antipyretics.
Coexistence of Hodgkin and Non-Hodgkin Lymphoma; Composite Lymphoma [CL] in a Patient Presenting with Waxing and Waning Lymphadenopathy
Authors: Geladari Eleni , Dimopoulou Georgia , Margellou Evangelia , Paraskevas Andreas , Kafetzis Georgios , Rontogianni Dimitra , Vadiaka Maria 
Published: October 2019

Background: :The coexistence of two or more types of lymphoma within the same organ at the same time of diagnosis is defined as composite lymphoma, a rare disease that has recently been identified in the literature. Pointedly, the concurrence may be Hodgkin lymphoma with a Non-Hodgkin lymphoma [NHL], either B or T cells, or two different entities of NHLs. Furthermore, this condition has been described concurrently or sequentially. In order for the diagnosis to be established, two or more distinct clones should be proven by morphological and laboratory tests.Case presentation::Herein, we cite a seventy-three-year old female patient with low-grade fever, waxing and waning cervical lymphadenopathy, whose biopsy of an axillary lymph node demonstrated the rare coexistence of Hodgkin and NHL, known as composite lymphoma.Conclusion: :Composite lymphomas pose a particular diagnostic challenge, and currently, there are no agreed standards for treatment.
Modul Pemesinan Gerinda Bagi Siswa SMK Teknik Pemesinan di Daerah Istimewa Yogyakarta
Authors: Saputra Fariz Hashfie , Purnomo Edy 
Published: October 2019

This research and development aims to determine the feasibility level of module learning media of grinding theory and to determine the effectiveness of basic grinding theory to the academic achievement of class XI students in the machining study program. The research methods is quantitative descriptive method. The results validates the use of grinding theory module in learning activities. The average percentage from the quality of content, instructional and technical, is 91,25%. The effectiveness of basic grinding theory module can be seen from the improvement of students’ marks of 97.28% in average. The increase in the active participation is 33.3%.Penelitian pengembangan ini bertujuan untuk mengetahui kelayakan media pembelajaran modul teori pemesinan gerinda dan mengetahui tingkat efektivitas modul teori pemesinan gerinda terhadap hasil belajar siswa teknik pemesinan kelas XI SMK Muhammadiyah Prambanan. Penelitian ini menggunakan langkah Research and Development dan metode penelitian deskriptif kuantitatif. Hasil pada penelitian ini adalah media pembelajaran modul teori pemesinan gerinda dinyatakan layak digunakan untuk kegiatan pembelajaran dengan rerata persentase kelayakan kualitas isi, instruksional, dan teknis sebesar 91,25%. Efektivitas media pembelajaran modul Teori Gerinda Dasar dapat dilihat dari peningkatan rerata hasil belajar sebesar 97,28% dan peningkatan partisipasi aktif sebesar 33,3%.
Pencegahan Perkawinan di Bawah Umur (Analisis Terhadap Lembaga Pelaksana Instrumen Hukum di Kec. Blangkejeren Kab. Gayo Lues)
Authors: Rahmatillah Syarifah , Nurlina Nurlina 
Published: October 2019

Undang-Undang Perkawinan, KHI dan Undang- Undang Perlindungan Anak telah menetapkan batas minimal usia perkawinan yaitu pria berusia 19 tahun dan wanita berusia 16 tahun. Untuk mendukung program ini maka lembaga pelaksana instrumen hukum seperti KUA, Dinas Syari’at Islam dan Dinas Pemberdayaan Perempuan dan Perlindungan Anak, Pengendalian Penduduk, dan Keluarga Berencana (DP3AP2KB) berupaya untuk mencegah pekawinan di bawah umur. Realitanya masih banyak terjadi perkawinan di bawah usia 16 tahun. Pertanyaan penelitian dalam kajian ini adalah apakah faktor yang mempengaruhi terjadinya perkawinan di bawah umur di masyarakat Kec. Blangkejeren, bagaimanakah praktik perkawinan di bawah umur yang di lakukan oleh masyarakat Kec.Blangkejeren, Bagaimanakah kekuatan lembaga pelaksana instrumen hukum dalam mencegah perkawinan di bawah umur di kec. Blangkejeren. Penelitian ini menggunakan metode kualitatif yang bersifat deskriptif. Hasil penelitian ditemukan bahwa Lembaga pelaksana instrumen Hukum yang berada di wilayah Kec. Blangkejeren belum maksimal untuk mengatasi praktik perkawinan di bawah umur disebabkan karena adanya faktor Orang tua, adat, Media massa, Pergaulan bebas, pemahaman agama yang masih dangkal. Dari paparan diatas dapat simpulkan bahwa, Praktik perkawinan di bawah umur yang dilakukan oleh masyarakat Kec. Blangkejeren adalah secara non prosedural tanpa melibatkan aparat-aparat institusi negara yang berwenang dan proses pencegahan perkawinan di bawah umur yang dilaksanakan oleh lembaga pelaksana instrumen hukum tidak akan berjalan maksimal tanpa didukung oleh para orang tua, tokoh adat, tokoh agama dan pemerintah dalam mencegah perkawinan di bawah umur.
Efektivitas Pelaksanaan Hukum Jinayat di Aceh
Authors: Yusuf Muhammad 
Published: October 2019

Efektif tidaknya sebuah aturan hukum dapat dilihat seberapa banyak masyarakat mematuhi aturan tersebut, jika sebuah aturan hukum dipatuhi lebih dari 50 (limapuluh) persen masyarakat sudah dapat dikatakan aturan tersebut efektif. Ukuran lainnya untuk melihat sebuah aturan hukum efektif adalah dari penurunan jumlah kasus pelanggaran hukum. Efektivitas sebuah aturan hukum sangat dipengaruhi oleh materi hukum itu sendiri, penegak hukum dan tingkat pemahaman masyarakat terhadap hukum. Dalam kajian ini, ukuran yang dipakai untuk melihat efektivitas hukum adalah dari segi jumlah kasus atau pelanggaran hukum yang dilakukan oleh masyarakat. Kemudian pengaruh efektivitas hukum dikaji dari sejauh mana pemahaman masyarakat terhadap hukum yang diberlakukan kepada mereka khususnya dalam hal ini adalah tentang hukum jinayat. Tingkat pemahaman masyarakat sangat ditentukan oleh keseriusan pihak yang bertanggungjawab mensosialisasikan hukum kedalam masyarakat. Khusus untuk hukum jinayat yang diberlakukan di Aceh, maka pihak yang sangat bertanggungjawab melaksanakan sosialisasi hukum adalah Dinas Syari’at Islam sebagai perpanjangan tangan Pemerintah Aceh.
PENYULUHAN PENTINGNY ASA YURAN BAGI ANAK-ANAK DI TK AISYIY AH KW ADUNGAN, TROW ANGSAN, MALANGJIW AN, COLOMADU, KARANGANY AR, JA WA TENGAH
Authors: Ichsan Burhannudin , Wibowo Bayu Hendro , Sidiq M. Nur 
Published: October 2019

Kindergarten children is a phase of school children. School children phase generally have better condition than children under five years, because many government programs can be targeted at these groups, that one of them is the UKS programs (Program Usaha Kesehatan Sekolah), but the program has not been prevalent at kindergarten school generally. A frequent problem faced by the children of this phase is difficult to eat, especially vegetables because they do not have a sweet taste. There are a variety of nutritional conditions that are unsatisfactory at schoolchildren, for example, less weight, iron deficiency anemia, a deficiency of vitamin C, and in certain areas also lack of jodium. Lack of vegetables are things that can cause vitamin and mineral deficiencies. Authors do counseling about the importance of vegetables for children in kindergarten Aisyiyah, Kwadungan, Trowangsan, Malangjiwan, Colomadu to convey the importance of vegetables so that the parents have a stronger effort to encourage their children to consume vegetables. Counseling is done by the power point media and done interactively. Authors gave pre-test andpost-test regarding knowledge about the importance of vegetables. There were prizes for the highest score of the post-test to attract participants. Paired samples t test was performed with the result that there was a significant increase in knowledge (p =0.000) in the parents.
PELATIHAN PEMANFAATAN BARANG BEKAS UNTUK PEMBUATAN BUKET BUNGA DAN CARA PEMASARANNYA
Authors: Astuti Indah Puji , Buntoro Ghulam Asyrofi , Ariyadi Dwiyono 
Published: October 2019

Waste is one of the problems that often arise in life society. However, many ways are considered wiser in solving problems with garbage. One of them is by using it to produce new products that have economic value. Not only can it reduce the garbage stack, but this method can also bring additional income. This service activity was carried out in the village of Bulu Lor, Jambon Subdistrict, Ponorogo Regency. Aim to provide training in making a bouquet by utilizing used items to homemakers and their youth. Besides, it also teaches how to market handicraft products to be known to more quickly, so the possibility of selling products is getting bigger. The result of this activity is to increase entrepreneurial skills to the community by providing capabilities to homemakers and youth in making flower bouquets and teaching how to market product results online. Keywords: Waste, bucket flower, entrepreneurship, online marketing
Pengaruh pesan instagram @Ricmaupdate terhadap minat remaja untuk menjadi anggota remaja islam masjid cut meutia
Authors: Azkia Verin Onella , Monterie Yudith Emanuella 
Published: October 2019

Nowadays, any kind of information can easily be found on social media platforms, one of them is through the platform of Instagram. On Instagram we can find further information of the RICMA organization that has been chosen as the main topic of this research. RICMA is a non-profit muslim organization that is family based. This research aims to be a medium between people and also persuasion is done with the use of the Instagram account @ricmaupdate to invite youth followers of the account to become a member of the organization. Thus, the research method was conducted with the use of quantitative method using the CMC theory (Computer Mediated Communication). The participants of this research are the @ricmaupdate 96 active followers on Instagram, supported with the purposive sampling technique as well as questionnaires as the data collection method. This research shows there is an influence from the messages from @ricmaupdate’s Instagram account on the interest of young adults that are becoming Islamic Youth of Cut Meutia Mosque. Keywords: Message; Instagram; Interest; Muslim Teenagers; Cut Meutie Mosque
Marco geológico e hidrológico de la mega-fauna fósil del pleistoceno, de la porción meridional del Estado de Hidalgo, México central
Authors: Ortiz Hernández Luis E. , Escamilla Casas José Cruz 
Published: October 2019

El marco geológico e hidrológico de la mega-fauna fósil del Pleistoceno (Mamuth, Equus sp. y Glyptodon) de la porción meridional del estado de Hidalgo (región Epazoyucan-Tecocomulco), es referido a un análisis de la distribución de los cauces hídricos remanentes y la red de drenaje actual, que muestra patrones de tipo radial centrífugo, dendrítico, subdendrítico, paralelo y sub-paralelo, correspondiente a la litología (rocas volcánicas y piroclásticas de tipo básico, intermedio y ácido) y sedimentos fluviales y lacustres del Pleistoceno tardío, así como al tipo de estructuras volcánicas presentes como son flujos de lava, flujos piroclásticos, domos, conos cineríticos, volcanes escudo y calderas del Mioceno al Pleistoceno-Cuaternario.
El análisis permite establecer que la estructura geológica regional (en bloques fallados) y la cercanía a grandes cuerpos de agua, fueron propicios al desarrollo de la mega-fauna fósil en esta región de México central.
Pressupostos pedagógicos da Educação a Distância: conhecendo as bases
Authors: Maia Gabrielle 
Published: October 2019

Resumo: O presente trabalho consiste na pesquisa bibliográfica das diversas teorias da Pedagogia que lançam base aos modelos utilizados na Educação a Distância apresentando assim os seus principais teóricos e pensamentos. Ao falarmos em Educação a Distância (EAD), ainda que no século XXI as tecnologias façam parte da vida cotidiana e a impregne de sentidos, não é difícil encontrar pessoas que desacreditem da qualidade deste ensino, seja pelo simples fato de desconhecerem as incontáveis vantagens que a modalidade oferece ou mesmo pela má impressão causada pela massificação da educação, vendida em larga escala e sem a devida preocupação com o que realmente se propõe. Faz-se necessário então, conhecer seus pressupostos pedagógicos para garantir assim a qualidade do ensino.Palavras-chave: Pressupostos pedagógicos. Educação a Distância. Pedagogia.
Expediente Revista EDaPECI
Authors: Revista EDaPECI Expediente 
Published: October 2019

Revista EDaPECI - Educação a Distância e Práticas Educativas Comunicacionais e Interculturais / Universidade Federal de Sergipe, São Cristóvão (SE). Revista EDaPECI, São Cristóvão (SE), v.17. n. 1, p.1-257, jan. / abr. 2017. Quadrimestral
CROP WILD RELATIVES IN MAGADAN PROVINCE OF RUSSIA: INVENTORYING AND CONSERVATION
Authors: Talovina G. V. , Aistova E. V. 
Published: October 2019

Background. Plant genetic resources (PGR) conservation is an indispensable condition for ensuring the country’s economic and environmental security. The most important component of the PGR status assessment is the study of crop wild relatives in a particular region. Within the framework of the work on inventorying crop wild relatives (CWR) in the regions of Russia, an inventory of CWR was for the first time made for Magadan Province, Far East region of Russia, to identify plant taxa that are prioritized for conservation and monitoring of their status in natural phytocenoses. Materials and methods. The material for the present research was taken from the data published on the vegetation of the studied region. Taxonomic analysis of CWR was performed using conventional floristic methods. In order to select priority species for conservation, the guidelines for PGR conservation adapted for Russia were used.Results and conclusions. A list of 232 CWR species belonging to 62 genera of 21 families has been compiled; it encompasses nearly 20% of the entire regional flora. The indigenous part of CWR amounts to ca. 60%, while the adventive part includes 40% of the total CWR number. Analysis of distribution of CWR species over the studied area showed that the greatest diversity of CWR species (81%) is concentrated in the Okhotsky floristic district. As a result of the analysis, 76 species occurring in Magadan Province were identified for prioritized in situ conservation.