bluuebunny/crossref_metadata_2025_split huggingface dataset:
The E3 ligase BRUTUS facilitates degradation of VOZ1/2 transcription factors
Authors: Selote Devarshi , Matthiadis Anna , Gillikin Jeffrey W. , Sato Masa H. , Long Terri A. 
Published: June 2018

AbstractBRUTUS (BTS) is an iron binding E3 ligase that has been shown to bind to and influence the accumulation of target basic helix‐loop‐helix transcription factors through 26S proteasome‐mediated degradation in Arabidopsis thaliana. Vascular Plant One‐Zinc finger 1 (VOZ1) and Vascular plant One‐Zinc finger 2 (VOZ2) are NAM, ATAF1/2 and CUC2 (NAC) domain transcription factors that negatively regulate drought and cold stress responses in plants and have previously been shown to be degraded via the 26S proteasome. However, the mechanism that initializes this degradation is unknown. Here, we show that BTS interacts with VOZ1 and VOZ2 and that the presence of the BTS RING domain is essential for these interactions. Through cell‐free degradation and immunodetection analyses, we demonstrate that BTS facilitates the degradation of Vascular plant One‐Zinc finger 1/2 (VOZ1/2) protein in the nucleus particularly under drought and cold stress conditions. In addition to its known role in controlling the iron‐deficiency response in plants, here, we report that BTS may play a role in drought and possibly other abiotic stress responses by facilitating the degradation of transcription factors, VOZ1/2.
The chimeric mitochondrial gene orf182 causes non‐pollen‐type abortion in Dongxiang cytoplasmic male‐sterile rice
Authors: Xie Hongwei , Peng Xiaojue , Qian Mingjuan , Cai Yicong , Ding Xia , Chen Qiusheng , Cai Qiying , Zhu Youlin , Yan Longan , Cai Yaohui 
Published: June 2018

SummaryD1‐cytoplasmic male sterility (CMS) rice is a sporophytic cytoplasmic male‐sterile rice developed from Dongxiang wild rice that exhibits a no‐pollen‐grain phenotype. A mitochondrial chimeric gene (orf182) was detected by mitochondrial genome sequencing and a comparative analysis. Orf182 is composed of three recombinant fragments, the largest of which is homologous to Sorghum bicolor mitochondrial sequences. In addition, orf182 was found only in wild rice species collected from China. Northern blot analysis showed that orf182 transcripts were affected by Rf genes in the isocytoplasmic restorer line DR7. Western blot analysis showed that the ORF182 product was localized in the mitochondria of the CMS line. An expression cassette containing orf182 fused to a mitochondrial transit peptide induced the maintainer line of male sterility, which lacked pollen grains in the anthers. Furthermore, the in vivo expression of orf182 also inhibited the growth of Escherichia coli, with lower respiration rate, excess accumulation of reactive oxygen species and decreased ATP levels. We conclude that the mitochondrial chimeric gene orf182 possesses a unique structure and origin differing from other identified mitochondrial CMS genes, and this gene is connected to non‐pollen type of sporophytic male sterility in D1‐CMS rice.
Stressful life events and resilience among carers of Aboriginal children in urban New South Wales: cross-sectional findings from the Study of Environment on Aboriginal Resilience and Child Health (SEARCH)
Authors: Young Christian , Craig Jonathan C , Clapham Kathleen , Williams Sandra , Williamson Anna 
Published: June 2018

ObjectiveIn caregivers of urban Aboriginal children, to determine the frequency of major stressful life events, the proportion who meet criteria for resilience, and factors that are associated with resilience.DesignCross-sectional survey.SettingFour Aboriginal Community Controlled Health Services located in urban or regional areas in New South Wales, Australia.Participants574 caregivers of Aboriginal children participating in the Study of Environment on Aboriginal Resilience and Child Health.Primary outcome measureResilience, defined as having experienced three or more stressful life events in the last 12 months, and having scores of ≤21 on the Kessler 10 Psychological Distress scale.ResultsOver half (315, 55%) of the caregivers reported three or more stressful life events—the most common being a close family member who was hospitalised with a serious medical problem (259, 45%). Of the participants who experienced three or more stressful life events, almost three-quarters (227, 72%) met the criteria for resilience. Using multivariable analysis, two factors were independently associated with resilience: not having a physical health problem that limited normal activities (adjusted OR (aOR) 4.3; 95% CI 2.0 to 9.0), and not having problems caused by alcohol within the home (aOR 5.3; 95% CI 2.2 to 12.8). Having a child whose behaviour placed a great deal of burden on the family was associated with less resilience (aOR 0.25; 95% CI 0.09 to 0.68).ConclusionsCaregivers of urban Aboriginal children experienced a large number of stressful events, the most common being the poor health of close family members, but most exhibited resilience. Resilience was associated with stable family environments and good physical health. The high number of stressful life events that caregivers experience is reflective of broader inequalities that Aboriginal communities face. The availability of easily accessible and long-term health and support services may go some way to reducing this inequality and improving social and emotional well-being for Aboriginal families.
Alternative payment models: can (should) trauma care be bundled?
Authors: Kerwin Andrew James , Mercel Alexandra , Skarupa David J , Tepas Joseph J , Ra Jin H , Ebler David , Hsu Albert , Shiber Joseph , Crandall Marie L 
Published: June 2018

BackgroundRecent legislation repealing the Sustainable Growth Rate mandates gradual replacement of fee for service with alternative payment models (APMs), which will include service bundling. We analyzed the 2 years’ experience at our state-designated level I trauma center to determine the feasibility of such an approach for trauma care.MethodsDe-identified data from all injured patients treated by the trauma service during 2014 and 2015 were reviewed to determine individual patient injury profiles. Using these injury profiles we created the ‘trauma bundle’ by concatenating the highest Abbreviated Injury Scale score for each of the six body regions to produce a single ‘signature’ of injury by region for every patient. These trauma bundles were analyzed by frequency over 2 years and by each year. The impacts of physiology and resource consumption were evaluated by determination of the correlation of the mean and SD of calculated survival probability (Ps) and intensive care unit length of stay (ICU LOS) for each profile group occurring more than 12 times in 2 years.ResultsThe 5813 patients treated over 2 years produced 858 distinct injury profiles, only 8% (71) of which occurred more than 12 times in 2 years. Comparison of 2014 and 2015 profiles demonstrated high frequency variation among profiles between the 2 years. Analysis of injury patterns occurring >12 times in 2 years demonstrated an inverse correlation between the mean and SD for Ps (R2=0.68) and a direct correlation for ICU LOS (R2=0.84).DiscussionThese data indicate that the disease of injury is too inconsistent a mix of injury pattern and physiologic response to be predictably bundled for an APM. The inverse correlation of increasing SD with increasing ICU LOS and decreasing Ps suggests an opportunity for measurable process improvement.Level of evidenceEconomic and value-based evaluations, level IV.Study typeEconomic/decision.
High definition maps in urban context
Authors: Zang Andi , Chen Xin , Trajcevski Goce 
Published: June 2018

Part of the challenges in the quest for smart cities is to enable effective navigation for different types of mobile users: from pedestrians, through drivers, to autonomous vehicles. While the data sources to facilitate such tasks abound, one of the pressing problems is how to enable efficient management and download of the data needed to populate the screens of devices with the appropriate visualization.
In this paper, we present an overview of the different issues and the main features of the existing technologies that, in one way or another, could be used as foundations for effective solution for generating quality maps. We also discuss the possible approaches for addressing such issues in the context of accurate self-localization of vehicles.
Novel methods of imaging and analysis for the thermoregulatory sweat test
Authors: Carroll Michael S. , Reed David W. , Kuntz Nancy L. , Weese-Mayer Debra E. 
Published: June 2018

The thermoregulatory sweat test (TST) can be central to the identification and management of disorders affecting sudomotor function and small sensory and autonomic nerve fibers, but the cumbersome nature of the standard testing protocol has prevented its widespread adoption. A high-resolution, quantitative, clean and simple assay of sweating could significantly improve identification and management of these disorders. Images from 89 clinical TSTs were analyzed retrospectively using two novel techniques. First, using the standard indicator powder, skin surface sweat distributions were determined algorithmically for each patient. Second, a fundamentally novel method using thermal imaging of forced evaporative cooling was evaluated through comparison with the standard technique. Correlation and receiver operating characteristic analyses were used to determine the degree of match between these methods, and the potential limits of thermal imaging were examined through cumulative analysis of all studied patients. Algorithmic encoding of sweating and nonsweating regions produces a more objective analysis for clinical decision-making. Additionally, results from the forced cooling method correspond well with those from indicator powder imaging, with a correlation across spatial regions of −0.78 (confidence interval: −0.84 to −0.71). The method works similarly across body regions, and frame-by-frame analysis suggests the ability to identify sweating regions within ~1 s of imaging. Although algorithmic encoding can enhance the standard sweat testing protocol, thermal imaging with forced evaporative cooling can dramatically improve the TST by making it less time consuming and more patient friendly than the current approach. NEW & NOTEWORTHY The thermoregulatory sweat test (TST) can be central to the identification and management of several common neurological disorders, but the cumbersome nature of the standard testing protocol has prevented its widespread adoption. In this study, images from 89 clinical TSTs were analyzed retrospectively using two novel techniques. Our results suggest that these improved methods could make sweat testing more reliable and acceptable for screening and management of a range of neurological disorders.
Macrophages Promote Circulating Tumor Cell–Mediated Local Recurrence following Radiotherapy in Immunosuppressed Patients
Authors: Rafat Marjan , Aguilera Todd A. , Vilalta Marta , Bronsart Laura L. , Soto Luis A. , von Eyben Rie , Golla Meghana A. , Ahrari Yasaman , Melemenidis Stavros , Afghahi Anosheh , Jenkins Melissa J. , Kurian Allison W. , Horst Kathleen C. , Giaccia Amato J. , Graves Edward E. 
Published: June 2018

Abstract
Although radiotherapy (RT) decreases the incidence of locoregional recurrence in breast cancer, patients with triple-negative breast cancer (TNBC) have increased risk of local recurrence following breast-conserving therapy. The relationship between RT and local recurrence is unknown. Here, we tested the hypothesis that recurrence in some instances is due to the attraction of circulating tumor cells to irradiated tissues. To evaluate the effect of absolute lymphocyte count on local recurrence after RT in patients with TNBC, we analyzed radiation effects on tumor and immune cell recruitment to tissues in an orthotopic breast cancer model. Recurrent patients exhibited a prolonged low absolute lymphocyte count when compared with nonrecurrent patients following RT. Recruitment of tumor cells to irradiated normal tissues was enhanced in the absence of CD8+ T cells. Macrophages (CD11b+F480+) preceded tumor cell infiltration and were recruited to tissues following RT. Tumor cell recruitment was mitigated by inhibiting macrophage infiltration using maraviroc, an FDA-approved CCR5 receptor antagonist. Our work poses the intriguing possibility that excessive macrophage infiltration in the absence of lymphocytes promotes local recurrence after RT. This combination thus defines a high-risk group of patients with TNBC.
Significance: This study establishes the importance of macrophages in driving tumor cell recruitment to sites of local radiation therapy and suggests that this mechanism contributes to local recurrence in women with TNBC that are also immunosuppressed.
Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/15/4241/F1.large.jpg. Cancer Res; 78(15); 4241–52. ©2018 AACR.
A Secondary Mutation inBRAFConfers Resistance to RAF Inhibition in aBRAFV600E-Mutant Brain Tumor
Authors: Wang Jiawan , Yao Zhan , Jonsson Philip , Allen Amy N. , Qin Alice Can Ran , Uddin Sharmeen , Dunkel Ira J. , Petriccione Mary , Manova Katia , Haque Sofia , Rosenblum Marc K. , Pisapia David J. , Rosen Neal , Taylor Barry S. , Pratilas Christine A. 
Published: June 2018

AbstractBRAFV600E hyperactivates ERK and signals as a RAF inhibitor–sensitive monomer. Although RAF inhibitors can produce impressive clinical responses in patients with mutant BRAF tumors, the mechanisms of resistance to these drugs are incompletely characterized. Here, we report a complete response followed by clinical progression in a patient with a BRAFV600E-mutant brain tumor treated with dabrafenib. Whole-exome sequencing revealed a secondary BRAFL514V mutation at progression that was not present in the pretreatment tumor. Expressing BRAFV600E/L514V induces ERK signaling, promotes RAF dimer formation, and is sufficient to confer resistance to dabrafenib. Newer RAF dimer inhibitors and an ERK inhibitor are effective against BRAFL514V-mediated resistance. Collectively, our results validate a novel biochemical mechanism of RAF inhibitor resistance mediated by a secondary mutation, emphasizing that, like driver mutations in cancer, the spectrum of mutations that drive resistance to targeted therapy are heterogeneous and perhaps emerge with a lineage-specific prevalence.Significance: In contrast to receptor tyrosine kinases, in which secondary mutations are often responsible for acquired resistance, second-site mutations in BRAF have not been validated in clinically acquired resistance to RAF inhibitors. We demonstrate a secondary mutation in BRAF (V600E/L514V) following progression on dabrafenib and confirm functionally that this mutation is responsible for resistance. Cancer Discov; 8(9); 1130–41. ©2018 AACR.See related commentary by Romano and Kwong, p. 1064.This article is highlighted in the In This Issue feature, p. 1047
Study on Dialysis Session Length and Mortality in Maintenance Hemodialysis Patients: The Q-Cohort Study
Authors: Fujisaki Kiichiro , Tanaka Shigeru , Taniguchi Masatomo , Matsukuma Yuta , Masutani Kosuke , Hirakata Hideki , Kitazono Takanari , Tsuruya Kazuhiko 
Published: June 2018

<b><i>Objectives:</i></b> Hemodialysis (HD) time has been recognized as an important factor in dialysis adequacy. However, few studies have reported on associations between HD time and prognosis among maintenance HD patients. We present some findings from a prospective cohort study, the ­Q-Cohort Study, which was set up to explore risk factors for mortality in Japanese HD patients. We hypothesized that HD ≥5 h was associated with a signiﬁcant survival advantage compared with HD &#x3c; 5 h. The present study examined association between HD time and mortality in Japanese HD patients. <b><i>Methods:</i></b> The prospective multicenter Q-Cohort Study was conducted between December 2006 and December 2010, following 3,456 Japanese HD patients for 4 years. We examined the association between HD time and prognosis using Cox proportional hazards modeling. Propensity scores were calculated using logistic regression. <b><i>Results:</i></b> During follow-up, 566 patients died from any cause. Patients with HD ≥5 h (<i>n</i> = 2,141) showed ­significantly lower risk of all-cause death (hazards ratio = 0.82; 95% CI 0.68–0.99) than those with HD &#x3c; 5 h (<i>n</i> = 1,315), after adjusting for confounding risk factors. This ­association remained significant using a propensity score-based approach. After stratifying the analysis by patient age in 10-year increments, this finding remained ­significant only in patients who were ≥80 years of age. <b><i>Conclusion:</i></b> Our results suggest that HD ≥5 h has a more favorable effect on mortality than HD &#x3c; 5 h.
Acute Kidney Injury before Dialysis Initiation Predicts Adverse Outcomes in Hemodialysis Patients
Authors: Lee Timmy , Shah Silvi , Leonard Anthony C. , Parikh Pratik , Thakar Charuhas V. 
Published: June 2018

<b><i>Background:</i></b> Acute kidney injury (AKI) is associated with increased morbidity and mortality. Mortality in end-stage renal disease (ESRD) patients is highest during the first year of dialysis. The impact of pre-ESRD AKI events on long-term outcomes in incident ESRD patients remains unknown. <b><i>Methods:</i></b> We evaluated a retrospective cohort of 47,341 incident hemodialysis patients from the United States Renal Data System with linked Medicare data for at least 2 years prior to hemodialysis initiation. We examined the impact of pre-ESRD AKI events in the 2-year pre-ESRD period on the type of vascular access used at hemodialysis initiation (central venous catheter (CVC) versus arteriovenous access), and 1-year all-cause mortality after initiating hemodialysis. <b><i>Results:</i></b> The mean age was 72 ± 11 years. Of the study cohort, 18% initiated hemodialysis with arteriovenous access, and 54% of patients had at least one pre-ESRD AKI event. One-year, all-cause mortality was 32%. Compared to 75% for patients without a pre-ESRD AKI event, 89% of patients with a pre-ESRD AKI event initiated hemodialysis with CVC than arteriovenous access (<i>p</i> &#x3c; 0.001). A pre-ESRD AKI event was associated with lower adjusted odds of starting hemodialysis with an arteriovenous access (OR 0.47; 95% CI 0.44–0.50, <i>p</i> &#x3c; 0.001), and higher adjusted odds of 1-year mortality (OR 1.36; 95% CI 1.30–1.42, <i>p</i> &#x3c; 0.001). <b><i>Conclusion:</i></b> An AKI event prior to initiating hemodialysis independently increases the risk of CVC use and predicts 1-year mortality. Improving processes of care after AKI events may improve dialysis outcomes in patients who progress to ESRD.
Systematic Review of Observational Studies with Dose-Response Meta-Analysis between Folate Intake and Status Biomarkers in Adults and the Elderly
Authors: Novaković Romana , Geelen Anouk , Ristić-Medić Danijela , Nikolić Marina , Souverein Olga W. , McNulty Helene , Duffy Maresa , Hoey Leane , Dullemeijer Carla , Renkema Jacoba M.S. , Gurinović Mirjana , Glibetić Marija , de Groot Lisette C.P.G.M. , van’t Veer Pieter 
Published: June 2018

<b><i>Background:</i></b><i></i> Dietary reference values for folate intake vary widely across Europe. <b><i>Methods:</i></b> MEDLINE and Embase through November 2016 were searched for data on the association between folate intake and biomarkers (serum/plasma folate, red blood cell [RBC] folate, plasma homocysteine) from observational studies in healthy adults and elderly. The regression coefficient of biomarkers on intake (β) was extracted from each study, and the overall and stratified pooled β and SE (β) were obtained by random effects meta-analysis on a double log scale. These dose-response estimates may be used to derive folate intake reference values. <b><i>Results:</i></b> For every doubling in folate intake, the changes in serum/plasma folate, RBC folate and plasma homocysteine were +22, +21, and –16% respectively. The overall pooled regression coefficients were β = 0.29 (95% CI 0.21–0.37) for serum/plasma folate (26 estimates from 17 studies), β = 0.28 (95% CI 0.21–0.36) for RBC (13 estimates from 11 studies), and β = –0.21 (95% CI –0.31 to –0.11) for plasma homocysteine (10 estimates from 6 studies). <b><i>Conclusion:</i></b> These estimates along with those from randomized controlled trials can be used for underpinning dietary recommendations for folate in adults and elderly.
МІСЦЕ ВАКУУМНОЇ ТЕРАПІЇ У ХІРУРГІЧНОМУ ЛІКУВАННІ ВАРИКОЗНОЇ ХВОРОБИ НИЖНІХ КІНЦІВОК В СТАДІЇ ДЕКОМПЕНСАЦІЇ
Authors: Goshchynsky V. B. , Kohan R. S. , Migenko B. O. , Herasimets Y. M. 
Published: June 2018

Вступ. 25 % хворих на варикозну хворобу мають ускладнення у вигляді трофічних виразок. Наявність трофічної виразки вимагає від хірурга проведення відповідної доопераційної підготовки, що безпосередньо збільшує матеріальні затрати та термін перебування хворого у стаціонарі.Мета. Дати обґрунтування до застосування вакуум-терапії в комплексі з хірургічним лікуванням варикозної хвороби, ускладненої трофічною виразкою, на доопераційному етапі.Матеріали та методи. Для передопераційної підготовки у 77 хворих з хронічною венозною недостатністю III ст., (СЕАР С6) була використана вакуум терапія трофічних виразок. Для обгрунтування застосування вакуум-терапії були проведено вивчення її впливу на загоєння трофічної виразки, стан її бактеріального забруднення, інтенсивність больвого синдрому, термін перебування у стаціонарі. Після очищення рани застосовували поліуретанову губку розміром пор в залежності від фази її загоєння. Так, під час фази запалення використовували поліуретанову губку з максимальним розміром пор 1500-2000 мкм і в режимі апарату (-300) мм рт. ст. Під час фази проліферації, використовували губку з розміром пор 500-1500 мкм і з режимом негативного тиску (-150) мм рт. ст. Під час фази загоєння, коли відбувається дозрівання рубцевої тканини і епітелізація рани, використовували губку з розміром пор до 500 мкм в режимі (-100) мм рт. ст.Результати досліджень. Аналіз ефективності застосування вакуум-терапії показав її переваги в плані термінів очищення або загоєння над традиційними методами лікування та значне скорочення до операційних днів.Після загоєння трофічних виразок або значного зменшення їх розмірів всім хворим була виконана ендовенозна лазерна абляція варикозно - трансформованих вен нижніх кінцівок. У 19 хворих була застосована методика надфасціальной лазерної коагуляції перфорантних вен під контролем ультразвукової діагностики, а у 23 пацієнтів була виконана субфасціальна ендоскопічна десекція перфорантних вен. У 19 хворих одночасно була використана аутодермопластика трофічної виразки.Висновки. 1. Застосування вакуум-терапії в комплексі хірургічного лікування хворих на варикозну хворобу нижніх кінцівок, ускладнену трофічною виразкою є сучасним і безальтернативним методом лікування, завдяки якому, як показують наші дослідження, знижується больовий синдром, а також кількість мікробних тіл і ранова ексудація, значно прискорюється розро-стання грануляційної тканини і стимулюється епітелізація.2. Застосування вакуум-терапії в якості передопераційної підготовки, скорочує тривалість перебування пацієнтів венозною недостатністю, ускладненою трофічної виразкою у стаціонарі.