bluuebunny/crossref_metadata_2025_split huggingface dataset:
Music Perception in Older Adults With Hearing Loss: Protective Effect of Musical Experience
Authors: Whittom Alexis , Chauvette Loonan , Bégin Alex , Blanchette Isabelle , Tremblay Pascale , Sharp Andréanne 
Published: December 2024

Objectives:
The goal of this project was to investigate the impact of musical experience, hearing loss, and age on music perception in older adults. The authors hypothesized that older adults with a varying degree of musical experience would perform better at music perception tasks than their counterparts without musical experience while controlling for age and hearing loss.

Design:
This study used a descriptive correlational cross-sectional design. Seventy-seven older adults aged 60 to 90 years were recruited and divided into two groups based on their lifetime musical experience: the group without musical experience (n = 39) and the M group (with musical experience; n = 38). Participants in the M group had either played an instrument for 5 years or more and/or taken at least 1 year of music lessons. Following a hearing screening and a musical experience questionnaire, participants completed two music perception tasks: (1) a short version of the Montreal Battery Evaluation of Amusia (MBEA) measuring melodic (scale and contour) and rhythm perception, and (2) an instrument discrimination task measuring timbre perception.

Results:
Results revealed that participants of the M group had a significantly higher accuracy in both tasks compared with the group without musical experience while controlling for age and hearing loss. Moreover, a significant interaction was found between group effect and hearing loss for the MBEA, suggesting that musical experience moderates the impact of hearing loss on melodic and rhythm perception abilities. Finally, the amount of musical experience was the most important positive predictor for MBEA accuracy in the M group.

Conclusions:
These results suggest that despite age-related hearing loss, older adults with musical experience still benefit from their experience-driven enhancement in melodic, rhythm, and timbre perception. Findings from this study support the notion that music training is beneficial for music perception abilities, providing protection against the impact of presbycusis.
Who is the PROM King? Patient-reported Outcome Measures in Pediatric Musculoskeletal Oncology: A Systematic Review
Authors: Baghdadi Soroush , Van Eenenaam David , Talwar Divya , Arkader Alexandre 
Published: December 2024

Background:
Patient-reported outcome measures (PROMs) are important companions to traditional, physician-reported measures. The purpose of this study was to systematically review and identify PROMs used in pediatric musculoskeletal oncology and evaluate these instruments.

Methods:
A systematic literature review was conducted for studies pertaining to pediatric musculoskeletal tumors from 2010 to 2024. Two experts selected eligible papers for inclusion through a systematic approach. PROMs used in the eligible papers were cataloged, and the top 10 most frequently used instruments were evaluated with Evaluating Measures of Patient-Reported Outcomes (EMPRO), which is a standardized evaluation tool for PROs.

Results:
A total of 316 publications were included in the final analysis, reporting a total of 82 unique PROMs. Of the papers, 24% reported no PROMs, whereas 67% of the instruments were only reported once. Nine out of the 10 top PROMs scored over 50 on EMPRO, meeting the minimum acceptable criteria. Musculoskeletal Tumor Society, Toronto Extremity Salvage Score, Disabilities of the Arm, Shoulder, and Hand (DASH), and Quick DASH were disease-specific instruments scoring high on EMPRO. Short Form-36, Pediatric Quality of Life Questionnaire, Quality of Life Questionnaire-30, BSI-18, and TNO AZL Child Quality Of Life met the requirements in the general instruments. Short Form-12 was the lowest-scored PROM.

Conclusions:
Our results show minimal consensus on the ideal PROM in pediatric musculoskeletal oncology. We recommend using instruments that are already in widespread use instead of obscure PROMs, which defy the purpose of reporting outcomes from a patient perspective. All 4 disease-specific instruments scored highly and are already widely used, including Musculoskeletal Tumor Society, Toronto Extremity Salvage Score, DASH, and Quick DASH. However, we found no consensus on general instruments. With the development and increasing popularity of Patient-Reported Outcomes Measurement Information System, it may replace other general instruments that are proprietary, unreliable, or not developed with pediatric patients in mind.

Level of Evidence:
Level IV—systematic review of level II to IV studies.
ICU Admission Preferences in the Hypothetical Event of Acute Critical Illness: A Survey of Very Old Norwegians and Their Next-of-Kins
Authors: Schwarz Gabriele Leonie , Skaar Elisabeth , Miljeteig Ingrid , Hufthammer Karl Ove , Burns Karen E. A. , Kvåle Reidar , Flaatten Hans , Schaufel Margrethe A. 
Published: December 2024

OBJECTIVES:
To explore older patients’ ICU admission preferences and their next-of-kins’ ability to predict these preferences.

DESIGN:
Self-administered survey.

SETTING:
Three outpatient clinics, urban tertiary teaching hospital, Norway.

PATIENTS:
Purposive sample of outpatients 80 years old or older regarded as potential ICU candidates and their next-of-kins.

INTERVENTIONS:
None.

MEASUREMENTS AND MAIN RESULTS:
We asked about the patients’ ICU admission preferences in three hypothetical scenarios of acute critical illness. Next-of-kin respondents were asked to make a proxy statement regarding the older respondents’ wishes regarding ICU admission. For each treatment choice, all respondents could provide their level of confidence. Additionally, we sought to identify demographic and healthcare-related characteristics that potentially influenced ICU admission preferences and proxy accuracy. Of 202 outpatients 80 years old or older, equal proportions opted for (39%; CI, 33–45%) and against (40%; CI, 34–46%) ICU admission, and one in five (21%; CI, 17–26%) did not wish to engage decision-making. Male gender, religiosity, and prior ICU experience increased the likelihood of older respondents opting for ICU admission. Although next-of-kins’ proxy statements only weakly agreed with the older respondents’ true ICU admission preferences (52%; CI, 45–59%), they agreed with the next-of-kins’ own ICU admission preferences (79%; CI, 73–84%) to a significantly higher degree. Decisional confidence was high for both the older and the next-of-kin respondents.

CONCLUSIONS:
In this purposive sample of Norwegian potential ICU candidates 80 years old or older, we found substantial variation in the ICU admission preferences of very old patients. The next-of-kins’ proxy statements did not align with the ICU admission preferences of the older respondents in half of the pairs, but next-of-kins’ and older respondents’ confidence levels in rendering these judgments were high.
Size-dependent Nanoparticle Accumulation In Venous Malformations
Authors: Cullion Kathleen , Ostertag-Hill Claire A. , Tang Weimin , Pan Michelle , Kohane Daniel S. 
Published: December 2024

Objective:
The current treatment of venous malformations (VMs) consists of medications with systemic toxicity and procedural interventions with high technical difficulty and risk of hemorrhage. Using nanoparticles (NPs) to enhance drug delivery to VMs could enhance efficacy and decrease systemic toxicity. NPs can accumulate in tissues with abnormal vasculature, a concept known as the enhanced permeation and retention (EPR) effect. EPR has been documented in tumors, bioengineered vessels, and VMs. However, in VMs, it is unknown if NP size affects EPR and if so, which particle size improves NP accumulation.

Methods:
In this study, we used a murine model of subcutaneous VMs using human umbilical vein endothelial cells that express the most frequent VM-causing tyrosine kinase with immunoglobulin and EGF homology domains mutation, tyrosine kinase with immunoglobulin and EGF homology domains-L914F. Hollow silica NPs coated in polyethylene glycol (PEG) and conjugated to a fluorophore were administered systemically via tail vein injection. We studied the accumulation of a range of NP sizes within the VM and organs using confocal microscopy and an in vivo imaging system.

Results:
The 20, 50, 80, and 180 nm PEGylated, fluorophore-tagged hollow silica NPs were spherical and had hydrodynamic diameters of 31.6 ± 0.9, 58.5 ± 0.1, 87.1 ± 2.4, and 232 ± 1.26 nm, respectively. Following systemic NP administration, 20 nm NPs had 2 times more fluorescence accumulation within VMs compared with 50 nm, and 6 times more fluorescence accumulation compared with larger (greater than 80 nm) NPs (P < .01).

Conclusion:
This study helps to determine the optimal NP size for passive accumulation within VMs and lays the foundation for engineering NPs for the treatment of VMs.
Is pT0 a Reliable End Point for Biomarker Discovery for Preoperative Chemotherapy Response in Patients With Bladder Cancer?
Authors: Sood Akshay , Lim Amy H. , Yao Hui , Wei Peng , Narayan Vikram M. , Lee I-Ling , Seif Mohammed A. , Bree Kelly K. , Matulay Justin T. , Campbell Matthew T. , Kamat Ashish M. , Dinney Colin P.N. , Navai Neema 
Published: December 2024

Purpose:
Traditional biomarker investigation schemas for chemotherapy response prediction in patients with bladder cancer relying on the pT0 status at radical surgery are confounded by the therapeutic effect of transurethral resection of bladder tumor (TURBT). Studying cN+ patients and assessing pN0 status presents a unique opportunity for overcoming this bias.

Materials and Methods:
We studied 26 patients with biopsy-proven cTanyN1-3M0 bladder cancer (2005-2016) who underwent induction chemotherapy and radical cystectomy with pelvic lymphadenectomy. Metastasis and overall survival (OS) outcomes were examined using Kaplan-Meier method and compared using log-rank test. Paired pretherapy primary bladder and nodal tissues were available for 10 patients. In these patients, whole-transcriptome RNA-seq analysis was performed on bladder tumor (for pT0 and pN0 prediction) and lymph-nodal metastasis (for pN0 prediction) tissues to identify differentially expressed genes (DEGs) with a false discovery rate < 0.1.

Results:
pN0 pathologic responders, but not pT0 responders, had significantly improved freedom from metastasis (5-year: pN0 88.9% vs pN+ 27.4%, log-rank P = .018) and OS (5-year: pN0 76.2% vs pN+ 12.2%, log-rank P = .024). Using RNA-seq data, we identified a significant discordance rate of 87.5% between DEG-based predictive signatures for pT0 and pN0 response to cisplatin-based chemotherapy. This datum, combined with the knowledge that ∼40% of patients who achieve pT0 status at radical surgery, achieve it simply through the therapeutic effect of TURBT (SWOG S8710), underscores a substantial bias in the current biomarker discovery initiatives that use pT0 as an end point.

Conclusions:
Our findings suggest a need for devising novel study designs to aid in the discovery of reliable biomarkers for preoperative chemo/immunotherapy response in bladder cancer. Clinical node-positive patients may be ideally situated but remain understudied.
Efficacy and Safety of Repeat Per-oral Endoscopic Myotomy After Failed POEM for Achalasia
Authors: Hayat Umar , Kamal Faisal , Rana Usman Iqbal , Iqbal Amna , Gangwani Manesh K. , Ali Hassam , Gopalakrishnan Keerthy , Farooq Umer , Akbar Hafiz M. , Kumar Kishore , Adler Doulas G. 
Published: December 2024

Background:
Per-oral endoscopic myotomy (POEM) is an effective option for treating achalasia. Despite its high efficacy, a fraction of patients remain symptomatic after POEM, and the data on the optimal management of these patients is limited. Few studies have evaluated the effectiveness of repeat POEM after a failed POEM.

Aim:
To evaluate the efficacy and safety of repeat POEM for persistent symptoms after the initial POEM.

Methods:
We searched major databases from inception to April 2024 to identify studies evaluating the efficacy and safety of repeat POEM after failed POEM in patients with achalasia. Our outcomes of interest were clinical success (a reduction in the Eckardt score to ≤3 after the procedure), adverse events, and differences in pre-procedure and post-procedure Eckardt scores. Using the random effects model, we calculated pooled rates with 95% CI for categorical variables and mean difference (MD) with 95% CI for continuous variables.

Results:
We included 6 observational studies with 123 patients. The mean time between myotomies ranged from 12.2 to 13.5 months. The pooled rate (95% CI) of clinical success was 82.69% (95% CI; 69.65%-93.06%). The pooled rate (95% CI) of adverse events was 17.97% (95% CI: 5.08%-35.15%). The pooled mean±SD post-procedure Eckardt score was 1.63±1.93. The mean post-procedure Eckardt score was significantly lower than the re-procedure Eckardt score, MD (95% CI): 3.68 (2.58, 4.78).

Conclusion:
Repeated POEM is an effective option for persistent symptoms of achalasia after initial POEM; however, it is associated with nontrivial adverse events.
miR-137 regulates autophagy and apoptosis in duodenal ulcer by targeting BNIP3L
Authors: Pan Zhaohui , Zhang Li , Hu Jing 
Published: December 2024

Background:
Duodenal ulcer (DU) represents a clinical manifestation and disease state that occurs when the mucosal surface of the duodenum is damaged. The processes of autophagy and apoptosis have been linked to the development of DU, yet the precise roles they play remain unclear. This study aimed to investigate the expression and mechanism of action of microRNAs (miRNA)-137 (miR-137) in DU.

Methods:
Dysregulated miRNAs and targeted genes were identified from the Gene Expression Omnibus database, and the immune cell infiltration levels were analyzed using CIBERSORT. To confirm the targeting of the miRNAs, we conducted dual luciferase reporter assays in vitro. The detection of cell apoptosis was conducted using flow cytometry. Moreover, quantitative reverse transcription polymerase chain reaction, cell counting kit-8, and Western blot were employed to ascertain the levels of autophagy- and apoptosis-related proteins.

Results:
Bioinformatics analysis identified 5 miRNAs, with miR-137 showing the most pronounced dysregulation. Its target gene, BNIP3L, was subsequently identified. In vitro experiments confirmed that miR-137 targeted BNIP3L. The upregulation of miR-137 expression in HIEC-6 cells resulted in the inhibition of BNIP3L expression, a reduction in autophagy, and an increase in apoptosis. A reduction in the expression of miR-137 would have the opposite effect.

Conclusions:
miR-137 is upregulated in DU patients and contributes to ulcer progression by inhibiting BNIP3L, reducing autophagy, and promoting apoptosis. Targeting miR-137 could provide a novel therapeutic strategy for DU management.
Tear cytokine levels in Sjogren’s syndrome-related dry eye disease compared with non-Sjogren’s syndrome-related dry eye disease patients: A meta-analysis
Authors: Li Jiaxi , Liu Yihe , Liu Ziyuan , Li Xuemin 
Published: December 2024

Background:
As a common complication of Sjogren syndrome (SS), SS-related dry eye disease (SS-DED) significantly affects the patients’ quality of life. Pro-inflammatory cytokines in tears are widely believed to play a crucial role in the pathogenesis of SS-DED. A systematic literature review with meta-analyses was conducted to provide a quantitative summary of tear cytokine levels in SS-DED compared with non-SS-DED and healthy controls.

Methods:
The PubMed, Embase, Web of Science, Ovid, and Scopus databases were searched until June 2022. Original case–control studies investigating tear cytokines in SS-DED patients compared with non-SS-DED or healthy individuals were included. Differences of cytokines levels were compared with random-effects standardized mean differences ± 95% confidence intervals calculated as the effect size.

Results:
A total of 15 articles, 809 subjects (302 for SS-DED, 220 for non-SS-DED, and 287 for healthy controls) were included in the study. SS-DED patients had higher tear levels of interferon-gamma, interleukin (IL)-17, IL-1β, IL-2, IL-4, IL-6, and IL-8 as compared to healthy controls. As for comparison between SS-DED and non-SS-DED group, the levels of IL-12p70, IL-17, IL-4, IL-6, IL-8, and tumor necrosis factor-alpha were significantly higher in SS-DED patients compared with the non-SS-DED group. The level of epidermal growth factor was significantly lower in SS-DED patients compared with both non-SS-DED patients and healthy controls.

Conclusion:
The findings from this study provide evidence for levels of tear cytokines in SS-DED to become potential diagnostic biomarkers or therapeutic targets. Further studies with a higher number of subjects and improved quality are necessary.
Construction of a prognostic model based on cuproptosis-related genes and exploration of the value of DLAT and DLST in the metastasis for non-small cell lung cancer
Authors: Ma Huiying , Ge Yizhi , Li Yuhong , Wang Tingting , Chen Wei 
Published: December 2024

Background:
To reveal the clinical value of cuproptosis-related genes on prognosis and metastasis in non-small cell lung cancer.

Methods:
Gene expression profiles and clinical information of non-small cell lung cancer were downloaded from The Cancer Genome Atlas and Gene Expression Omnibus databases. The data were grouped into training set, internal testing set, and external testing set. A risk prognostic model was constructed by Lasso-Cox regression analysis. Hub genes were identified and evaluated using immunohistochemistry and the transwell migration assay in 50 clinical patients.

Results:
A total of 17/19 cuproptosis-related genes were differentially expressed in tumors, 8 were significantly associated with prognosis, and 4 were markedly associated with metastasis. A risk model based on 2 cuproptosis-related genes was constructed and validated for predicting overall survival. The risk score was proven to be an independent risk factor for the prognosis of non-small cell lung cancer. Dihydrolipoamide S-acetyltransferase and dihydrolipoamide S-succinyltransferase, key genes in cuproptosis, were proven to be associated with non-small cell lung cancer prognosis and metastasis. Immunohistochemistry showed that their expression significantly predicted metastasis but failed to predict prognosis in non-small cell lung cancer patients. The transwell migration assay further increased the cellular reliability of our findings.

Conclusion:
The cuproptosis-related genes prognostic model effectively predicted the prognosis of non-small cell lung cancer. Dihydrolipoamide S-acetyltransferase and dihydrolipoamide S-succinyltransferase may serve as predictive markers for metastasis in non-small cell lung cancer.
Summary of clinical features of 1800 cases of pyogenic liver abscess
Authors: Tian Yu , Hao Diandian , Du Jialin , Wang Hui , Wen Xiaoyu 
Published: December 2024

Objective
This study aimed to summarize the clinical and microbiological characteristics of patients with pyogenic liver abscess (PLA) and to explore the clinical features of PLA with extrahepatic migratory infection (EMI).

Methods
A retrospective analysis was conducted on clinical data from 1800 PLA patients at Jilin University First Hospital from January 2019 to December 2023. Patients were divided into two groups based on the presence of EMI: with EMI and without EMI. Clinical features and prognoses of the two groups were compared using rank-sum tests and chi-square tests for continuous and categorical data, respectively.

Results
PLA patients were predominantly male (65.56%) with an average age of 60. Abscesses were mainly located in the right lobe (64.83%) and were often single (68.17%). Klebsiella pneumoniae was the primary pathogen (68.46%), with 9.50% of strains being multidrug-resistant. The majority of patients improved with effective treatment (96.17%). Compared with the non-EMI group, patients with EMI were younger, had longer hospital stays, smaller abscesses, and a higher incidence of diabetes and cerebrovascular disease, with poorer prognoses.

Conclusion
PLA is most commonly observed in middle-aged and elderly males, often presenting as single abscesses in the right lobe, with diabetes as a frequent underlying condition. Most patients recover with appropriate antibiotic treatment and ultrasound-guided drainage. PLA patients with EMI generally have poorer outcomes and require special attention.
The Influence of Burnout, Resilience, and Resources to Support Clinical Practice Among Newly Licensed Nurses Intended Job Plans
Authors: Gregory Katherine E. , Babicheva Viktoriya , Chen Elizabeth , McTernan Melissa , Alberti Traci L. , Waddell Ashley , Meedzan Nancy , Warshawsky Nora 
Published: December 2024

OBJECTIVE
Survey newly licensed nurses (NLNs) with respect to burnout, resilience, perception of the work environment, and intended short- and long-term job plans.

BACKGROUND
NLN retention is a significant contributing factor to the nursing shortage.

METHODS
A 126-item survey measuring demographics, burnout, resilience, work environment, and job plans distributed via email, using Listservs to recruit nurses licensed for 5 years or less.

RESULTS
Participants (N = 547) reported moderate burnout, specifically emotional exhaustion, and resilience. Overall scores on perception of the work environment were positive. Multiple logistic regression suggests odds of 1-year intended job flight are positively associated with emotional exhaustion (odds ratio [OR], 1.77; P < 0.001), resilience (OR, 1.07; P = 0.003) and non-White racial identity (OR, 2.99; P < 0.001) and negatively associated with staffing and resource adequacy (OR, 0.63; P = 0.038).

CONCLUSIONS
Retaining NLNs requires mitigating burnout, addressing the experience of non-White NLNs in clinical practice, and improving support and resources for NLNs.
Human-Centered Design in Graduate Nursing Education
Authors: Holt Jeana M. , Talsma AkkeNeel , Lloren Jan Irene C. , Eljarrah Imteyaz , Woehrle Lynne , Avdeev Ilya 
Published: December 2024

Background:
Many nursing curricula lack human-centered design (HCD) learning opportunities, and minimal evidence exists about HCD educational outcomes.

Purpose:
The study explored the effects of HCD experiential learning activities on graduate nursing students.

Methods:
The quasi-experimental mixed-method design employed an explanatory approach. Pre- and posttest surveys were administered during an HCD workshop. Descriptive and inferential statistics assessed gains in the measures. Qualitative data were collected once, post-intervention, and analyzed using thematic analysis.

Results:
Sixty-four students provided complete responses. A linear mixed-effects regression model indicated statistically significant gains in creative self-efficacy, psychological empowerment, and traits of design thinkers. Qualitative themes contextualized the quantitative findings.

Conclusions:
The results indicated that HCD experiential learning influenced the study’s constructs. These results set the stage for multi-site studies investigating the long-term outcomes of HCD interventions in nursing curricula.