bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growth, pigment, and chromophoric dissolved organic matter responses of tropical Chattonella subsalsa (Raphidophyceae) to nitrogen enrichment
Authors: Kok Jerome W.K. , Yeo Darren C.J. , Leong Sandric C.Y. 
Published: December 2018

SUMMARYBlooms of the raphidophyte Chattonella subsalsa have been associated with massive fish‐kill events in several parts of the world. However, there have been few studies into physiological responses of tropical strains that could contribute to bloom outcomes. Such knowledge could provide insight into the C. subsalsa blooms recently documented within tropical coastal waters (e.g., 2010 and 2012 events in Singapore). Strains used in this study were isolated from the East Johor Straits (EJS), Singapore, an enclosed water channel frequently subjected to high levels of eutrophication. These cells were classified within the ‘global’ clade (and distinct from the ‘Adriatic Sea’ clade) based on morphology. The present study examined cellular responses to varying inputs of different forms of nitrogen (N), specifically nitrate, ammonium, and urea. Results from the study indicated that cells were unable to utilize urea as an N‐source, but grew well on a nitrate (Vmax = 0.73 day−1) and ammonium (Vmax = 0.81 day−1) supply. These growth rates were high compared to other strains from around the world, indicating that tropical C. subsalsa could exhibit elevated bloom potential within frequently eutrophic environments such as the EJS. Six pigments were detected in all cultures. These pigments were chlorophylls a and c; fucoxanthin; diadinoxanthin; violaxanthin; and β‐carotene. Chlorophyll‐a and fucoxanthin were the dominant pigments under both nitrate and ammonium regimes. Measurements of chromophoric dissolved organic matter generally increased both in molecular weight and in total content across the N‐concentration ranges. Such outcomes could have consequences for the chemical and optical conditions of the coastal environment.
Global prevalence of dental caries in athletes with intellectual disabilities: An epidemiological systematic review and meta‐analysis
Authors: Navarro Azevedo de Azeredo Fellipe , Silva Guimarães Ludmila , Azeredo A. Antunes Lívia , Santos Antunes Leonardo 
Published: December 2018

AbstractAimsThis study aimed to perform an epidemiological systematic review and meta‐analysis to estimate the global prevalence of dental caries in athletes with intellectual disabilities.Methods and resultsThis study was registered in PROSPERO (CRD42017068127). A systematic and extensive search was conducted in the Pubmed, Scopus, Web of Science, Virtual Health Library (Lilacs) databases, and Grey literature from inception up to April 2017. The MeSH terms “Sports,” Athletes,” and “Dental Caries” were used. The inclusion criteria used were observational cross‐sectional studies, longitudinal retrospective and prospective studies that present the prevalence of dental caries in athletes with intellectual disabilities. After the selection process, the risk of bias was assessed and a meta‐analysis was conducted. Sixteen articles met the inclusion criteria. Of these, eight studies were considered to have a moderate risk of bias, and eight were considered to have high risk of bias. The global prevalence of dental caries was 36.5% (95% CI = 30.6 to 42.7) with high heterogeneity between studies.ConclusionDental caries is a significant global health problem in athletes with intellectual disabilities worldwide. There is a great need for increased oral health promotion, education, and prevention as a way to reduce this condition in this population.
Long‐run determinants and misalignments of the real effective exchange rate in the EU
Authors: Comunale Mariarosaria 
Published: December 2018

AbstractThis paper explores the role of fundamentals, included in the transfer effect theory, in explaining medium/long‐run movements in the real effective exchange rates in the EU over the period 1994–2012, together with an analysis of the misalignments. We use heterogeneous, cointegrated panel frameworks in static and a dynamic setup. We find that the coefficients of the determinants are extremely different across groups and the transfer theory does not always hold. The core countries have been undervalued for almost the whole period; the periphery has experienced high rates. The misalignments in the CEECs are still wide and reflect the catching‐up process.
Videofluoroscopy, CT angiography, and ultrasonography of congenital esophageal stenosis in a Labrador retriever
Authors: Kopke Matthew A. , Gal Arnon , Ruaux Craig G. , Gans Leah C. , Aberdein Danielle , Owen Mark C. , Burchell Richard K. 
Published: December 2018

AbstractA 7‐week‐old Labrador retriever presented for further investigation into acute onset regurgitation, following weaning from liquid to solid food. A videofluoroscopic swallow study demonstrated a severe, focal esophageal dilation in the mid‐cervical region, with marked luminal narrowing distally. Computed tomography with angiography revealed esophageal stenosis, extending from C4–T2, secondary to circumferential esophageal wall thickening. With the concern for development of secondary aspiration pneumonia, the owners elected to euthanize the dog and consented to postmortem examination. A diagnosis of segmental, cervical esophageal muscular hypertrophy was made on necropsy, consistent with the fibromuscular thickening type of congenital esophageal stenosis reported in humans.
European Prevention of Alzheimer’s Dementia Longitudinal Cohort Study (EPAD LCS): study protocol
Authors: Solomon Alina , Kivipelto Miia , Molinuevo José Luis , Tom Brian , Ritchie Craig W 
Published: December 2018

IntroductionThe European Prevention of Alzheimer’s Dementia (EPAD) project is funded initially by the Innovative Medicines Initiative and has been established to overcome the major hurdles hampering drug development for secondary prevention of Alzheimer’s dementia, by conducting the EPAD Longitudinal Cohort Study (LCS) in alignment with the Bayesian adaptive designed EPAD Proof-of-Concept (PoC) trial.Methods and analysisEPAD LCS is an ongoing prospective, multicentre, pan-European longitudinal cohort study. Participants are recruited mainly from existing parent cohorts across Europe to form a ‘probability-spectrum’ population covering the entire continuum of anticipated probability for Alzheimer’s dementia development. The primary objective of the EPAD LCS is to be a readiness cohort for the EPAD PoC trial though a second major objective is to generate a comprehensive and large data set for disease modelling of preclinical and prodromal Alzheimer’s disease. This characterisation of cognitive, biomarker and risk factor (genetic and environmental) status of research participants over time will provide the necessary well-phenotyped population for developing accurate longitudinal models for Alzheimer’s disease covering the entire disease course and concurrently create a pool of highly characterised individuals for the EPAD PoC trial.Ethics and disseminationThe study has received the relevant approvals from numerous Institutional Review Boards across Europe. Findings will be disseminated to several target audiences, including the scientific community, research participants, patient community, general public, industry, regulatory authorities and policy-makers. Regular and coordinated releases of EPAD LCS data will be made available for analysis to help researchers improve their understanding of early Alzheimer’s disease stages and facilitate collaborations.Trial registration numberNCT02804789.
Comparison of standardised mortality ratios for renal failure before and after the 2011 Great East Japan Earthquake and Tsunami: an analysis of national vital statistics
Authors: Tsukinoki Rumi , Murakami Yoshitaka , Kawado Miyuki , Hashimoto Shuji 
Published: December 2018

ObjectiveThe impact of the 2011 Great East Japan Earthquake on renal failure (RF) risk remains unclear. We examined the 1-year impact of this disaster on RF mortality.SettingThis ecological study focused on the year before and after the earthquake. The data sources were national vital statistics (2010–2012), the national census (2010) and the Basic Resident Registration (2010–2012).ParticipantsOur study included all residents in Iwate, Miyagi and Fukushima, 1 year before and after the earthquake.Primary and secondary outcome measuresWe calculated standardised mortality ratios (SMRs) for RF, chronic RF and acute RF. Postearthquake weekly SMRs were calculated using the number of RF deaths for the corresponding weeks in 2010 as a reference. The SMRs for RF were compared between the coastal and inland municipalities using kernel-weighted polynomial smoothing.ResultsThere were 1290 RF deaths in the three prefectures during the year after the earthquake (chronic RF: 804 and acute RF: 236). The SMR for RF increased significantly in the first week after the earthquake in coastal areas (3.11; 95% CI: 1.84 to 4.37), but did not increase in inland areas (0.93; 95% CI: 0.47 to 1.38). A similar trend was observed for chronic RF (coastal: 4.0; 95% CI: 2.0 to 6.0; inland: 1.1; 95% CI: 0.4 to 1.7). SMRs for RF and chronic RF decreased over time and reached 1.0 approximately 20 weeks after the disaster. Changes in SMRs for acute RF were not apparent due to the low number of deaths.ConclusionsMortality due to RF and chronic RF, but not acute RF, increased in coastal areas after the earthquake. Chronic RF may have been exacerbated by disaster-induced sympathetic activation and poor management of renal dysfunction. Increased hypertension and damage to essential infrastructure and medical equipment may also have increased mortality in people with kidney disease.
Impact of genetic testing on low-density lipoprotein cholesterol in patients with familial hypercholesterolemia (GenTLe-FH): a randomised waiting list controlled open-label study protocol
Authors: Nomura Akihiro , Tada Hayato , Okada Hirofumi , Nohara Atsushi , Ishikawa Hideki , Yoshimura Kenichi , Kawashiri Masa-aki 
Published: December 2018

IntroductionFamilial hypercholesterolemia (FH) is an autosomal-dominant inherited genetic disease. High-throughput sequencing quickly and comprehensively detects causative variants of FH-related genes (LDLR,PCSK9,APOBandLDLRAP1). Although the presence of causative variants in FH-related genes correlates with future cardiovascular events, it remains unclear whether detection of causative gene mutation and disclosure of its associated cardiovascular risk affects outcomes in patients with FH. Therefore, this study intends to evaluate the efficacy of counselling future cardiovascular risk based on genetic testing in addition to standard patients’ education programme in patients with FH.Methods and analysisA randomised, waiting-list controlled, open-label, single-centre trial will be conducted. We will recruit patients with clinically diagnosed FH without previous history of coronary heart disease from March 2018 to December 2019, and we plan to follow up participants until March 2021. For the intervention group, we will perform genetic counselling and will inform an estimated future cardiovascular risk based on individuals’ genetic testing results. The primary endpoint of this study is the plasma low-density lipoprotein cholesterol level at 24 weeks after randomisation. The secondary endpoints assessed at 24 and 48 weeks are as follows: blood test results; smoking status; changes of lipid-lowering agents’ regimen and Patients Satisfaction Questionnaire Short Form scores among the four groups divided by the presence of genetic counselling and genetic status of FH.Ethics and disseminationThis study will be conducted in compliance with the Declaration of Helsinki, the Ethical Guidelines for Medical and Health Research Involving Human Subjects and all other applicable laws and guidelines in Japan. This study protocol was approved by the IRB at Kanazawa University. We will disseminate the final results at international conferences and in a peer-reviewed journal.Trial registration numberUMIN000029375.
What do European women know about their female cancer risks and cancer screening? A cross-sectional online intervention survey in five European countries
Authors: Wegwarth Odette , Widschwendter Martin , Cibula David , Sundström Karin , Portuesi Rosalba , Lein Ines , Rebitschek Felix G 
Published: December 2018

ObjectivesInformed decisions about cancer screening require accurate knowledge regarding cancer risks and screening. This study investigates: (1) European women’s knowledge of their risk of developing breast, ovarian, cervical or endometrial cancer, (2) their knowledge about mammography screening and (3) whether an evidence-based leaflet improves their knowledge.DesignCross-sectional online intervention survey.SettingNational samples from five European countries (Czech Republic, Germany, UK, Italy and Sweden)—drawn from the Harris Interactive and the Toluna panel, respectively, in January 2017—were queried on their knowledge of age-specific risks of developing breast, cervical, ovarian or endometrial cancer within the next 10 years and of mammography screening before and after intervention.ParticipantsOf 3629 women (inclusion criteria: age 40–75 years) invited, 2092 responded and 1675 completed the survey (response rate: 61.4%).InterventionEvidence-based leaflet summarising information on age-adjusted female cancer risks, mammography and aspects of cancer prevention.Primary outcome measuresProportion of women (1) accurately estimating their risk of four female cancers, (2) holding correct assumptions of mammography screening and (3) changing their estimations and assumptions after exposure to leaflet.FindingsAcross countries, 59.2% (95% CI 56.8% to 61.6%) to 91.8% (95% CI 90.3% to 93.0%) overestimated their female cancer risks 7–33 fold (mediansacross tumours: 50.0 to 200.0). 26.5% (95% CI 24.4% to 28.7%) were aware that mammography screening has both benefits and harms. Women who accurately estimated their breast cancer risk were less likely to believe that mammography prevents cancer (p<0.001). After leaflet intervention, knowledge of cancer risks improved by 27.0 (95% CI 24.9 to 29.2) to 37.1 (95% CI 34.8 to 39.4) percentage points and of mammography by 23.0 (95% CI 21.0 to 25.1) percentage points.ConclusionA considerable number of women in five European countries may not possess the prerequisites for an informed choice on cancer screening. Evidence-based information in patient leaflets can improve this situation.
Validity and reliability of maternal recall of pregnancy history and service use among signing Deaf women: a cross-sectional descriptive study from South Africa
Authors: Fontes Marx Mayara , Heap Marion , Gichane Margaret W , London Leslie 
Published: December 2018

IntroductionThere is little credible quantitative data on pregnancy histories and outcomes for disabled women in low-income and middle-income countries. The purpose of this study, based in Cape Town, South Africa, was to test the reliability and validity of maternal recall of pregnancy history and service use among a sample of Deaf women who use South African Sign Language (SASL).MethodsWe interviewed 42 signing Deaf women of childbearing age (18–49 years) in SASL using a structured questionnaire in July 2016. To assess reliability, seven participants (16% of the sample) were reinterviewed by different interviewers under the same conditions after 10–30 min. For the analysis we used (1) Cohen’s kappa, an inter-rater statistical method, and (2) overall percentage agreement. Validity was explored by comparing the participants’ pregnancy history to the Western Cape Provincial Health Data Centre (PHDC) database.ResultsThe reliability results showed that out of 19 questions 14 demonstrated substantial to perfect agreement kappa scores (kappa between 0.61 and 1) and 5 had the lowest kappa agreement scores (kappa <0.61). With respect to percentage agreement, participants provided identical responses in 87% cases. Overall, women provided more reliable responses to pregnancy outcomes compared with demographic information. Validity results showed that 29 out of 35 Deaf women provided survey responses that matched or nearly matched (83% agreement) the PHDC database for birth history and delivery location.ConclusionThis study suggests that for this sample of signing Deaf women recall of pregnancy history and service use is reliable and valid. Extending this approach to other similar populations will require further research, but it is important that methods to access hard-to-reach disabled populations are developed so that health system responsiveness to marginal populations can be based on robust evidence.
Patient-centred care for addiction treatment: a scoping review protocol
Authors: Marchand Kirsten , Beaumont Scott , Westfall Jordan , MacDonald Scott , Harrison Scott , Marsh David C , Schechter Martin T , Oviedo-Joekes Eugenia 
Published: December 2018

IntroductionSubstance use disorders are chronic conditions that require a multidimensional treatment approach. Despite ongoing efforts to diversify such treatments, evidence continues to illuminate modest rates of treatment engagement and perceived barriers to treatment. Patient-centred care (PCC) is one approach that may strengthen the responsiveness of treatments for people with problematic substance use. The aim of this scoping review is to explore how the principles of PCC have been implemented and operationalised in healthcare settings for people with problematic substance use.Methods and analysisThis scoping review follows the iterative stages of the Arksey and O’Malley framework. Both empirical (from Medline, Embase, PsycINFO, CINAHL and ISI Web of Science) and grey literature references will be considered if they focused on populations with problematic substance use and described or measured PCC or one of its principles in a health-oriented context. Two reviewers will independently screen references in two successive stages of title/abstract screening and then full-text screening for references meeting title/abstract criteria. A descriptive overview, tabular and/or graphical summaries, and a directed content analysis will be carried out on extracted data. This scoping review has been registered with Open Science Framework (https://osf.io/5swvd/).Ethics and disseminationThis review will systematically examine the extent and nature of existing evidence of PCC in addiction research and clinical practice. Such evidence will contribute to the operationalisation of PCC for people with problematic substance use. A multidisciplinary team has been gathered to represent the needs of people with problematic substance use, healthcare providers and decision-makers. The team’s knowledge users will be engaged throughout this review and will participate in dissemination activities (eg, workshops, presentations, publications, reports).
Evaluating associations between metabolic health, obesity and depressive symptoms: a prospective analysis of data from the English Longitudinal Study of Ageing (ELSA) with a 2‑year follow‑up
Authors: Slater Natasha , Rowley Charlotte , Venables Rebecca Hayley , White Simon , Frisher Martin 
Published: December 2018

ObjectivesConflicting results have been reported when the associations between metabolic health, obesity and depression were examined previously. The primary aim of this study was to determine whether metabolic health or obesity are independently associated with depressive symptoms, among a representative sample of older people living in England. Independent associations between covariates and depression were also examined.DesignProspective study with a 2-year follow-up.SettingThe English Longitudinal Study of Ageing Wave 6 (2012–2013) and Wave 7 (2014–2015).Participants6804 participants aged older than 50 years.Data AnalysisMultivariate models were used to determine whether metabolic health or obesity are independently associated with depressive symptoms at 2-year follow-up. Unadjusted and adjusted ORs with corresponding 95% CI were calculated; the adjusted ORs took account of baseline depression, gender, age, wealth, obesity and poor metabolic health.ResultsBefore adjusting for covariates, poor metabolic health was associated with depressive symptoms at 2-year follow-up (OR 1.24; 95% CI, 1.07 to 1.44, p<0.01). After adjusting for covariates, the association was no longer statistically significant (OR 1.17; 95% CI, 0.99 to 1.38, p=0.07). Similarly, obesity was associated with depressive symptoms at 2-year follow-up before adjusting for covariates (OR 1.54; 95% CI, 1.33 to 1.79, p<0.01). However, after adjusting for covariates the association between obesity and depressive symptoms at 2-year follow-up became statistically insignificant (OR 1.19; 95% CI, 1.00 to 1.41, p=0.06). The strongest predictors for future depression were baseline depression (OR 10.59; 95% CI, 8.90 to 12.53, p<0.01) and lower wealth (OR 3.23; 95% CI, 2.44 to 4.35, p<0.01).ConclusionNeither poor metabolic health nor obesity were associated with a risk of depressive symptoms at 2-year follow-up, after adjusting for covariates. As wealth inequalities continue to rise across England, the risk of depressive symptoms at 2-year follow-up is likely to be elevated in individuals living in the lower wealth quintiles.
Exploring the precision of femtosecond laser-assisted descemetorhexis in Descemet membrane endothelial keratoplasty
Authors: Pilger Daniel , von Sonnleithner Christoph , Bertelmann Eckart , Maier Anna-Karina B , Joussen Antonia M , Torun Necip 
Published: December 2018

ObjectiveDescemet membrane endothelial keratoplasty (DMEK) remains a challenging technique. We compare the precision of femtosecond laser-assisted DMEK to manual DMEK.Methods and AnalysisA manual descemetorhexis (DR) of 8 mm diameter was compared with a femtosecond laser-assisted DR of the same diameter (femto-DR) in 22 pseudophakic patients requiring DMEK. We used OCT images with a centred xy-diagram to measure the postoperative precision of the DR and the amount of endothelial denuded area. Endothelial cell loss (ECL) and best corrected visual acuity were measured 3 months after surgery.ResultsIn the manual group, the median error of the DR was 7% (range 3%–16%) in the x-diameter and 8% (range 2%–17%) in the y-diameter. In the femto group, the median error in the respective x and y-diameters was 1% (range 0.4%–3%) and 1% (range 0.006%–2.5%), smaller than in the manual group (p=0.001). Endothelial denuded areas were larger in the manual group (11.6 mm2, range 7.6–18 mm2) than in the femto group (2.5 mm2, range 1.2–5.9 mm2) (p<0.001). The ECL was 21% (range 5%–78%) in the manual DR and 17% (range 6%–38%) in the femto-DR group (p=0.351). The median visual acuity increased from 0.4 logMAR (range 0.6–0.4 logMAR) in both groups to 0.1 logMAR (range 0.4–0 logMAR) in the manual group and to 0.1 logMAR (range 0.3–0 logMAR) in the femto group (p=0.461). Three rebubblings were required in the manual group, whereas the femto group required only one.ConclusionThe higher precision of the femto-DR bears the potential to improve DMEK surgery.