bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the Mechanisms of the Ferroptosis-Related Gene TGFBR1 in Autoimmune Uveitis Based on Machine Learning Models
Authors: Zhang Danlei , Ren He , Xing Yiqiao , Chen Zhen 
Published: October 2024

Abstract

Autoimmune uveitis (AU) is a group of autoimmune-driven diseases characterized by intraocular inflammation, often leading to severe vision loss. Ferroptosis, a recently discovered form of programmed cell death, has not yet been fully explored in the pathogenesis of AU. This study aims to investigate the role of ferroptosis-related key genes in AU, providing a theoretical foundation for further mechanistic studies. We downloaded GSE198533 dataset from the Gene Expression Omnibus (GEO). Through differential gene expression (DEG) analysis, weighted gene co-expression network analysis (WGCNA), and two machine learning models, TGFBR1 and ZFAS1 were identified as critical hub genes. Additionally, we validated the expression of TGFBR1 in retinal inflammation in a mouse model of experimental autoimmune uveitis (EAU) and explored its functional role. The results showed that TGFBR1 expression was significantly downregulated in EAU. Moreover, TGFBR1 overexpression exacerbated retinal inflammation via the GPX4-mediated ferroptosis pathway, accompanied by decreased FOXP3 expression and elevated IL-17A levels, suggesting that TGFBR1 may aggravate the inflammatory response in Behçet's disease (BD) by influencing immune regulatory mechanisms. The correlation between TGFBR1 expression and disease severity highlights its potential as a therapeutic target for EAU and a biomarker for BD, offering promising avenues for molecular-targeted therapies in AU.
Stepwise leaching rare earth from weathered crust elution-deposited rare earth ores by the inhibition leaching of aluminum with HMTA
Authors: He Zhengyan , Zuo Qin , Tang Yuchen , Cui Wenqian , xu zhigao , Wu Ming , Qu Jun , Chi Ruan 
Published: October 2024

Abstract

In the leaching process of rare earth (RE) from weathered crust elution-deposited rare earth ores (WCE-DREO), the exchangeable impurity ions will also be exchanged and enter the RE leachate. Impurity removal must be treated before precipitating RE, which often causes the serious loss of RE and environmental pollution. Therefore, anti-impurity leaching of RE from WCE-DREO was investigated with hexamethylenetetramine (HMTA) as an impurity inhibitor and ammonium sulfate (AS) as a leaching agent. HMTA was first injected into the RE ores at a concentration of 0.8 wt.%, liquid/solid of 0.5:1 and natural pH. Then, the injection solution was replaced with 2 wt.% AS at liquid/solid of 0.5:1 and natural pH. The leaching efficiency of impurity Al could decrease to 5.99%. Without impurity removal, the RE leachate can be directly precipitated to obtain RE products with high quality. The leaching efficiency of RE was 92.95%, which was slightly higher than the traditional leaching technology with a single AS. The stepwise anti-impurity leaching technology simplifies the recovery process of RE, which benefits the green and high-efficiency exploitation of WCE-DREO. The physicochemical properties of RE ores and tailings were analyzed by modern analytical techniques to reveal the anti-impurity leaching mechanism. In the HMTA anti-impurity stage, HMTA can promote the conversion of exchangeable Al to adsorbed hydroxyl Al in RE ores. The adsorbed hydroxyl Al thinly attached to the surface of mineral particles is hard to be leached by leaching agent and retained in the RE ore in the AS leaching stage.
Viral IL-10 regulates nasopharyngeal carcinoma cell progression via the JAK1/STAT3 signalling pathway
Authors: Qiu Qin , Yang Xingyu , Li Xiaojiang , Ren Yanxin , Ning Huang 
Published: October 2024

Abstract

Purpose Nasopharyngeal carcinoma is a common clinical malignant tumour in the nasopharynx. The secretion of vIL-10 by EB virus can promote the development of nasopharyngeal carcinoma. The purpose of this study was to provide a theoretical basis for potential targets and mechanisms of vIL-10 in nasopharyngeal carcinoma.
Methods A total of 100 ng/mLvIL-10 was applied to the nasopharyngeal carcinoma CNE-2 cell line for 48 h,and then whole transcriptome sequencing analysis was performed to identifypotential targets and signalling pathways of vIL-10 innasopharyngeal carcinoma. The effects of vIL-10 on the proliferation, migration, invasion and apoptosis of human nasopharyngeal carcinoma cells were determined by ELISA, Ki67 immunofluorescence, colony formation, Transwell migration/invasion, Hoechst 33258 staining, flow cytometry and other experiments, and the potential effects of vIL-10 on nasopharyngeal carcinoma cells were verified at the cellular level. Western blotting was performed to measure the changes in key factors of the JAK1-STAT3 signalling pathway in nasopharyngeal carcinoma cells after vIL-10 treatment.
Results The whole transcriptome gene sequencing results showed that vIL-10 could effectively activate the JAK-STAT signalling pathway and upregulatethe expression of JAK1 and STAT3. Moreover, vIL-10 inhibited the apoptosis of nasopharyngeal carcinoma cells, enhanced their migration and invasion capabilities, and further promoted the proliferation of nasopharyngeal carcinoma cells.
Conclusion VIL-10 regulates the JAK1-STAT3 signalling pathway, promotes the proliferation of NPC cells, enhances their migration and invasion capabilities, inhibits tumour cell apoptosis, and participates in the progression of nasopharyngeal carcinoma.
Correlation of Suspicious And Pathological Cardiotocography (CTG) with Umbilical Cord Blood Gas Parameters: A Prospective Cohort Study
Authors: Paikaray Soumyashree , Jena Saubhagya Kumar , Balakrishnan Deepthy , Mohanty Pankaj Kumar 
Published: October 2024

Abstract

Introduction-Fetal blood sampling is considered the best option in overcoming the increased false positivity of CTG but is not used in many developing countries due to logistics difficulties. Hence, obstetricians highly rely on CTG for diagnosing fetal hypoxia. Studies of suspicious and pathological CTG correlating with umbilical blood parameters are limited. Lactate can be a reliable marker to assess intrapartum hypoxia. However, the correlation of suspicious and pathological CTG with lactate is least studied.
Methodology- A prospective observational cohort study was conducted to correlate suspicious and pathological CTG with umbilical cord arterial blood gas parameters in induced or laboring pregnant women and to compare the maternal and neonatal outcomes.
Results- Out of 2350 women subjected to continuous CTG, 104 and 101 participants had suspicious and pathological CTG, respectively. A statistically significant association of CTG was seen in women with preterm delivery and with the use of tramadol. The association of primary outcomes like pH, base excess, and lactate with CTG findings was significant.
Discussion- Other modalities to confirm acidosis should be used before an intervention in women with suspicious CTG. Drugs like tramadol and agents used for induction may play a role in abnormal CTG tracing in suspicious CTG. Cord blood lactate has a stronger association with CTG when compared with other umbilical cord parameters.
Conclusion- Pathological CTG should be taken cautiously as it carries a greater risk of perinatal distress, but suspicious CTG tracing needs to be supplemented with additional tests before any interventions.
DARS2 Promotes Bladder Cancer Progression by Enhancing PINK1-Mediated Mitophagy
Authors: Li Dongqing , Su Hang , Huang Yuan , Wang Zihuan , Zhang Jinge , Chen Chen , Hou Lina , Tan Wanlong , Li Fei 
Published: October 2024

Abstract

Background: Bladder cancer is the tenth most common cancer worldwide. Mitochondria, as central hubs of cellular metabolism, play a crucial role in various diseases and cancers. However, the role and underlying mechanisms of mitophagy, the key process governing mitochondrial quantity and quality control, remain unclear in bladder cancer.
Methods: 824 mitophagy-related genes were collected from the Genecards database. Construction and validation of the mitophagy-related genes prognostic model were completed by univariate Cox analysis, Lasso and SuperPC algorithms, Kaplan-Meier survival, univariate and multifactorial Cox regression. The screening and intervention of DARS2 were done by univariate Cox regression, Monocle3 pseudotime and DepMap analysis as wel as siRNA and shRNA. Mitophagy was detected by flow cytometry analysis, super-resolution microscopy, confocal microscopy and transmission electron microscopy.
Results: We screened 16 mitophagy-related genes to construct a prognostic model, which demonstrated strong predictive performance for overall survival, immune response, and chemotherapy outcomes in bladder cancer patients. Among these genes, DARS2 was identified as having a particularly significant impact on bladder cancer prognosis. Further investigation revealed that DARS2 promotes G1-to-S phase transition by upregulating CDK4 expression, thereby inhibiting cellular senescence and promoting cell proliferation. Additionally, DARS2 enhanced PINK1 expression in bladder cancer cells, leading to increased PINK1-mediated mitophagy. Importantly, both in vitro and in vivo experiments confirmed that DARS2 inhibits cellular senescence and promotes tumor progression by enhancing PINK1-mediated mitophagy.
Conclusions: DARS2 promotes PINK1-mediated mitophagy and facilitates G1-to-S phase transition, thereby delaying cellular senescence and promoting bladder cancer progression. Targeting DARS2 and mitophagy could be a promising therapeutic strategy to improve the prognosis of bladder cancer patients.
Importin 7 Mediated Nuclear Transport of MSI2 as a Therapeutic Target in Cervical Cancer
Authors: Qinyang Xu , zhou Wan-Zhen , Qiu Tian , Wang Juan , cheng jing , Jiang Rongzhen , Ai Zhihong , Teng Yincheng , Ma Li , Zhang Rui , Bai Yun 
Published: October 2024

Abstract

Cervical cancer (CC) remains a significant health threat to women worldwide, with a pressing need for novel therapeutic targets. Despite recent advances, the molecular mechanisms underlying CC progression are not fully understood. Nuclear transport proteins, key regulators of macromolecule movement between cellular compartments, have emerged as potential targets in cancer therapy. However, the specific role of Importin 7 (IPO7) in CC development and its clinical implications remain poorly characterized, indicating a significant gap in our current understanding. Here, we investigated IPO7's role in CC, leveraging clinical samples, bioinformatics analyses from TCGA and GEO databases, and experimental models. We found that IPO7 is upregulated in CC and associated with poor prognosis. IPO7 knockdown in cell lines and animal models revealed decreased cell proliferation, reduced colony formation, inhibited migration and invasion, and enhanced apoptosis. To uncover IPO7's molecular mechanisms, we performed mass spectrometry analysis, identifying MSI2, an RNA-binding protein, as a potential cargo. Further validation confirmed a direct interaction between IPO7 and MSI2, with IPO7 overexpression coupled with MSI2 knockdown abrogating oncogenic effects. Sequencing analysis of IPO7-knockdown cells indicated the MYC targets pathway and co-immunoprecipitation assays confirmed a direct interaction between MYC and MSI2, suggesting that IPO7 may facilitate the nuclear transport of MSI2 and MYC, thereby promoting cancer progression. Clinically, elevated MSI2 expression in CC patients, particularly in advanced stages, correlated with poorer outcomes. Our findings elucidate the role of IPO7 in CC, demonstrating its potential as a therapeutic target. The interaction between IPO7, MSI2, and MYC provides a novel avenue for developing targeted therapies. Importantly, our results underscore the importance of IPO7-mediated nuclear transport in CC progression, presenting a promising strategy for enhancing patient outcomes and advancing CC treatment.
On-treatment platelet reactivity at low shear stress and platelet activation status on aspirin or clopidogrel monotherapy in patients with TIA or ischaemic stroke
Authors: Lim Soon Tjin , Stephen Murphy , Smith Deirdre , Collins Ronan , Coughlan Tara , Murphy Sinead , McCarthy Alan , Egan Bridget , Lim Su-Yin , Cox Dermot , McCabe Dominick 
Published: October 2024

Abstract

Background
Simultaneously-collected data regarding platelet reactivity and activation status in ischaemic cerebrovascular disease (CVD) patients commencing commonly-prescribed antiplatelet agents are limited.
Methods
This prospective pilot observational study assessed TIA/ischaemic stroke patients before (baseline; N = 73), and 14 +/-7 days (14d; N = 59) and ≥ 90 days (90d; N = 38) after commencing aspirin or clopidogrel monotherapy. Platelet reactivity at low shear-stress in whole blood (Multiplate® Aspirin/ADP assays) and platelet activation status (% expression of CD62P, CD63 and leucocyte-platelet complexes on whole blood flow cytometry) were quantified. Prevalence of high on-treatment platelet reactivity (HTPR) was determined on the Multiplate analyser with ‘case-control definitions’ (Aspirin-HTPR: > 40U on Aspirin assay; Clopidogrel-HTPR: > 46 U on ADP assay), and innovative ‘longitudinal definitions’ of HTPR (failure to reduce aggregation on the relevant assay compared with the patient’s own baseline value by more than twice the co-efficient of variation of the assay).
Results
The case-control prevalence of aspirin-HTPR was 23.8% (5/21) at 14d, and 30.8% (4/13) at 90d, with corresponding values for longitudinal aspirin-HTPR of 4.8% (1/21) at 14d, and 0% at 90d. The case-control prevalence of clopidogrel-HTPR was 60.5% (23/38) at 14d, and 52% (13/25) at 90d, which was significantly higher than the prevalence of longitudinal clopidogrel-HTPR of 21% (8/38) at 14d, and 24% (6/25) at 90d (P ≤ 0.03), respectively. % Neutrophil-platelet complexes (P = 0.04) and % lymphocyte-platelet complexes (P = 0.002) were higher in patients with vs. those without case-control clopidogrel-HTPR at 14d, but not at 90d. The median % lymphocyte-platelet complexes significantly decreased between baseline and 14d (2.35% vs. 1.24%, P = 0.019), and median % monocyte-platelet complexes decreased between baseline and 90d (6.5% vs. 5.1%, P = 0.017) only in the clopidogrel-patient subgroup whose platelets were adequately inhibited by clopidogrel’. Two of four patients with recurrent vascular events had case-control clopidogrel-HTPR at 14d.
Conclusions
An important proportion of CVD patients had antiplatelet-HTPR at low shear-stress on the Multiplate assay. The prevalence of antiplatelet-HTPR is higher with case-control than with innovative longitudinal definitions. Quantification of % leucocyte-platelet complexes improves our understanding of the cellular mechanisms contributing to traditional case-control clopidogrel-HTPR in CVD patients.
Prevalence and antimicrobial susceptibility pattern of gram- negative bacteria contaminating the hands of patients’ visitors at regional referral hospitals in Dar-es-Salaam: A hospital based cross sectional study
Authors: Jonas Ninael , Mkenda Donath , Killel Erick , Henry Stanslaus , Magembe Emmanuel , Abednego Reuben , Urio Loveness , Lyamuya Eligius 
Published: October 2024

Abstract

Background
Hand contamination by bacteria is a significant source of infection transmission, especially in hospital settings. A healthcare-associated infection is one that a person contracts as a result of their interaction with a hospital. Health care workers’, patients’ and visitors’ hands are all transmission routes for infections, in particular bacterial infections. These infections elevate the economic burden on healthcare systems especially in low-income settings. The purpose of this study is to determine the prevalence of gram-negative bacteria hand contamination among patients’ visitors of referral hospitals in Dar es Salaam.
Results
Prevalence of gram-negative bacterial contamination on visitors’ hands was 91 (21.1%) on entry and 103 (30.2%) on exit. The most common bacteria contamination was from Klebsiella pneumoniae on both entry and exit points, 37 (41%) and 57 (43%) respectively. Resistance to cephalosporins (Cefotaxime and Ceftriaxone) were the most pronounced. Proportion of ESBL-producing bacteria was significantly higher at exit than at entry. Bacterial contamination was associated with not washing hands APR = 1.5 (95% CI:1.03–2.17), offering services to the patient APR = 1.9 (95% CI:1.21–2.87) and longer hospital stays (more than 7 days) APR = 1.5 (95% CI:1.1-2.0).
Conclusion
To prevent the transmission of bacteria, it is important to emphasize hand hygiene and exposure limits for visitors entering hospital environments.
Usefulness perinatal prognosis assessment using z-score over current low birth weight metric: a descriptive study
Authors: Murano Yayoi , Shoji Hiromichi , Sassa Miho , Sakamaki Ken , Shimizu Toshiaki , Gilmour Stuart , Yoneoka Daisuke 
Published: October 2024

Abstract

Background: A cut-off weight of 2500 g in infants, which is commonly referred as low birth weight infants, was established as an indicator of perinatal prognosis several decades ago.
However, perinatal medicine developed dramatically since then and theoutcomes are discussed using other factors including fetal growth nowadays.
This study was conducted to assess the present perinatal situation with recent assessment scales and reconsider the indicators used for perinatal outcome.
Methods: This is a retrospective descriptive study analyzing whole Japanese birth certificate data between 1992 and 2018. Trend in prenatal outcomes including birth weight, gestational age, presence of fetal growth restriction, preterm birth and birth weight-to-gestational age z-score were calculated. Fetal growth restriction was defined as birth weight-to-gestational age z-score less than 10 percentile.
Results: A total of 48,235,783 births in Japan were included. The proportion of infants with birth weight under 2500 g was constant while mean birth weight stopped declining and mean gestational age continued to fall. In contrast, the incidence of fetal growth restriction and the mean birth weight-to-gestational age z-score were improving. In detail, proportion of early term infants (between 37 weeks 0 day and 38 weeks 6 days of gestational age) was increasing while that of late preterm infants (between 34 weeks 0 day and 37 weeks 6 days of gestational age) was constant.
Conclusions: Over all perinatal outcome was improving. Shortened gestational age result from change in perinatal management policy due to progress in perinatal medicine that enabled smaller infants and/or infants born at shorter gestational age to survive. However, even if delivered with a shorter gestational period with an aim to achieve good outcomes, considering that the 10th percentile weight of a baby boy born to a primiparous mother at 37 weeks 0 days gestation, a full-term delivery, is 2203 g, for example, the infants would be classified as a low birthweight infant which is an indicator for poor prognosis. Therefore, low birth weight no longer reflects perinatal outcome appropriately and other factors like fetal growth should be considered as indicators for overall perinatal context.
Association of Chlamydia Trachomatis Sero-Positivity with Male Infertility in the Niger-Delta Region of Nigeria: A Case-Control Study
Authors: Asaboro Newton , Omo-Aghoja Lawrence Osuakpor , Adeyinka Ayotunde 
Published: October 2024

Abstract

Background
The role of Chlamydia infection in the causation of male infertility has not been conclusively evaluated, especially in the developing countries of sub-Sahara Africa that are worse affected by infertility. Understanding the relationship between the two will guide the deployment of preventive efforts and investigative approach more scientifically.
Methods
This was a case-control study of two groups of males – 68 male partners of infertile couples with abnormal semen parameters attending fertility clinic and 68 age-matched male partners (with normal semen parameters) of females attending the family planning clinic as controls at two tertiary hospitals in Delta state. The prevalence of chlamydial seropositivity, the sociodemographic characteristics, sexual & reproductive characteristics, and chlamydial serology of all the participants were determined and compared using appropriate statistical tools.
Results
Most (41.2%) of the participants were in the 35–39 years’ age category, were all married and were mostly (66.2%) skilled workers. The overall prevalence of Chlamydia seropositivity in this study was 11.8%. The prevalence of Chlamydia seropositivity was significantly higher among cases (19.1%) compared with controls (4.4%); (P < 001). Chlamydial seropositivity was significantly associated with oligospermia and teratozoospermia. On bivariate analysis, being in the infertile group (Crude OR 5.12, P = 0.008), history of a genital ulcer (Crude OR 0.124. P = 0.042), and history of dysuria (P = 0.008) were significantly associated with chlamydial trachomatis seropositivity. Upon multivariate logistic regression analysis, none of these covariates was found to be independent predictor of Chlamydia trachomatis seropositivity.
Conclusion
There was a significant association between Chlamydia trachomatis infection and abnormal semen parameters. There were no identified independent predictors of chlamydia trachomatis sero-positivity. Chlamydial screening should be incorporated as part of the basic male fertility investigations.
Analysis of the current situation and influencing factors of work family support for shared nurses in Shanxi Province：A cross-sectional study
Authors: wang xuan , xu YuHong , wu Jing , li JuanJuan , lili gao , Zhang NianNian , Wu ChunYan , Guo ShuMing , Zhang ZhiXia 
Published: October 2024

Abstract

Background
China is experiencing an aging population, resulting in a huge demand for "Internet plus + nursing services", a new service model. However, there are many risk problems in the process of shared nurse service. In order to improve the willingness of shared nurses to continue to participate, work family support scale is used to assess the problems shared nurses face in "Internet plus nursing service".
Aims
To assess the level of work-family support among shared nurses and to analyze the current situation and the factors that influence it.
Methods
A descriptive cross-sectional survey will be conducted from March to May 20234, using convenience sampling to select 360 shared nurses from Linfen City, Changzhi City, and Taiyuan City in Shanxi Province as survey subjects. Use the Work Family Support Questionnaire developed by Li Yongxin.
Results
The cumulative score of this study is (116.38 ± 26.81), and the average score of each item is (3.93 ± 0.87). Through multiple linear regression analysis, it was determined that hospital level, number of orders taken by online appointment nurses, and per capita annual household income have a significant impact on the work family support of online appointment nurses (P < 0.05).
Conclusion
The work-family support scores of shared nurses in China are high. Hospital level, the number of orders received by shared nurses, and per capita annual household income are the key factors affecting the work-family support scores of shared nurses. Hospitals and the government should improve the admission standards and audit mechanisms for shared nurses, establish a sound safety and security mechanism for shared nurses' travel, and strengthen the laws and systems about shared nurses.
Clinical trial number: not applicable.