bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isoaspartate formation and irreversible aggregation of collapsin response mediator protein 2: implications for the etiology of epilepsy and age-related cognitive decline
Authors: Zhu Jeff X. , Aswad Dana W. 
Published: November 2024

Abstract

Collapsin response mediator protein 2 (CRMP2) functions in the genesis and activity of neuronal connections in mammalian brain. We previously reported that a protein coincident with CRMP2 on 2D-gels undergoes marked accumulation of abnormal L-isoaspartyl sites in brain extracts of mice missing the repair enzyme, protein L-isoaspartyl methyltransferase (PIMT). To confirm and explore the significance of isoaspartyl damage in CRMP2, we expressed and purified recombinant mouse CRMP2 (rCRMP2). A polyclonal antibody made against the recombinant protein precipitated CRMP2 from brain extracts of PIMT-KO mice, but not from WT mice, suggesting that (1) the rCRMP2 antigen underwent significant isoAsp formation in the process of antibody production and (2) the isoAsp form of CRMP2 is considerably more immunogenic than the native protein. In vitro aging of rCRMP2 at pH 7.4, 37°C for 0–28 days led to robust accumulation of isoAsp sites that were repairable by PIMT, and also induced a progressive accumulation of apparent dimers and higher-mass oligomers as judged by SDS-PAGE. A similar pattern of CRMP2 aggregation was observed in mice, with levels increasing throughout the lifespan. We conclude that CRMP2 is indeed a major target of PIMT-mediated protein repair in the brain; that isoAsp forms of CRMP2 are highly immunogeni; and that CRMP2 dysfunction makes a significant contribution to neuropathology in the PIMT-KO mouse.
The effectiveness of interventions that support women, girls, and people who menstruate to participate in physical activity: a rapid overview of reviews
Authors: Csontos Judit , Brown Natalie , Edwards Adrian , Edwards Deborah , Gillen Elizabeth , Hounsome Juliet , Kiseleva Meg , Lewis Ruth , Macey Steven , Mann Mala , Sidhu Amrita , Cooper Alison 
Published: November 2024

Abstract

Background
Adults (age 18–64 years) are recommended 150–300 minutes of moderate-to-vigorous or 75–150 minutes of vigorous-intensity physical activity per week. Irrespective, the number of women not meeting recommended physical activity is 5% higher than men globally. Women, girls and people who menstruate face multiple barriers to participating in physical activity, including gender bias, low perceived exercise competence, and insufficient support from peers and/or family. Moreover, menstruation is often reported as a barrier. Numerous interventions have been proposed to increase physical activity participation of women and girls, while little is known about interventions for people who menstruate. Therefore, the aim of this rapid overview of reviews was to investigate the effectiveness of interventions that support women, girls, and people who menstruate to participate in physical activity. Additionally, this review examined whether any of these interventions incorporated managing physical activity participation throughout the menstrual cycle.
Methods
Bibliographic databases (MEDLINE, Emcare, and AMED on OVID platform; CINAHL and SPORTDiscus on EBSCO; Epistemonikos, and Cochrane Database) and grey literature were searched in March 2024. Title/abstract screening was conducted by one reviewer and 20% of records checked by a second. Full-texts screening was performed by two reviewers. Data extraction and critical appraisal (using JBI systematic review checklist) were conducted by one reviewer with another checking accuracy. Findings were summarised narratively.
Results
Fifteen systematic reviews across 16 reports (published between 2008–2024) met the inclusion criteria. The population included young and adolescent girls (n = 9), adult women (n = 3), mothers and daughters (n = 2), and mixed population (n = 1). A wide range of interventions were identified which were educational, environmental or multicomponent. None of the included systematic reviews described interventions focusing on managing physical activity participation throughout the menstrual cycle. Most reviews found mixed effectiveness in increasing physical activity participation, leading to inconclusive results.
Conclusion
There is a need for further research to better support women, girls, and people who menstruate to participate in physical activity, with particular focus on addressing barriers related to the menstrual cycle. The findings can inform workplace, educational and other relevant physical activity recommendations for women, girls and people who menstruate.
Analyzing the Impact of Symbols in Taiwan’s Anti-Disinformation Campaign on TikTok during Elections
Authors: Bhattacharya Sayantan , Agarwal Nitin , Poudel Diwash 
Published: November 2024

Abstract

Social, cultural, and political (SCP) symbols in campaigns play vital roles in connecting emotionally with people, representing identity, simplifying complex issues, and reinforcing political ideologies. This study investigates the impact of SCP symbol content on engagement, emotional responses, and trust in electoral process in TikTok-based anti-disinformation campaigns during Taiwanese 2024 general elections. Utilizing advanced natural language processing and machine learning, we analyzed posts and comments, categorizing them for presence and type of SCP symbols. We employed a multi-faceted approach, examining engagement metrics, emotional responses, and trust evaluations across different symbol categories. Our novel categorization ranged from content without symbols to those incorporating multiple symbol types, providing nuanced understanding of symbolic influence. Findings reveal a strong positive correlation between the complexity of symbolic content and user engagement, with cultural symbols emerging as potent in driving interaction and fostering trust. Emotional analysis showed symbol-rich content elicited more positive emotional responses, especially with cultural symbols. Trust evaluations showed increases in symbol diversity increased trust, with cultural and political symbols generating the highest trust levels towards Taiwan’s democratic and electoral process. Statistical validation through non-parametric tests confirmed that the observed effects were not artifacts of data distribution or follower count variations. Our findings have significant implications for public affairs, strategic communications, policymakers, and platform managers, providing a framework for crafting more 1 effective, culturally resonant content in digital socio-political campaigns. This study also opens avenues for further research into the long-term effects of symbolic communication on political behavior and democratic participation in the digital age.
Enhanced Therapeutic Effects of Hypoxia-Preconditioned Mesenchymal Stromal Cell-Derived Extracellular Vesicles in Renal Ischemic Injury
Authors: Yuan Fei , Liu Jie , Zhong Liang , Liu Pengtao , Li Ting , Yang Kexin , Gao Wei , Zhang Guangyuan , Sun Jie , Zou Xiangyu 
Published: November 2024

Abstract

Background
Extracellular vesicles (EVs) secreted by mesenchymal stromal cells (MSCs) provide significant protection against renal ischemia-reperfusion injury (IRI). Hypoxia is considered an important method for enhancing the tissue repair capabilities of MSCs. However, the specific effects of hypoxia on MSCs and MSC-EVs, as well as their therapeutic potential for renal IRI, remain unclear. In this study, we investigated the alterations in MSCs and the production of MSC-EVs following hypoxia pre-treatment, and further explored the key intrinsic mechanisms by which hypoxic MSC-EVs treat renal IRI.
Methods
Human umbilical cord MSCs were cultured under normoxic and hypoxic conditions. Proliferation and related pathways were measured, and RNA sequencing was used to detect changes in the transcription profile. MSC-EVs from both normoxic and hypoxic conditions were isolated and characterized. In vivo, the localization and therapeutic effects of MSC-EVs were assessed in a rat renal IRI model. Histological examinations were employed to assess the structure, proliferation, and apoptosis of IRI kidney tissue respectively. Renal function was measured by analyzing serum creatinine and blood urea nitrogen levels. In vitro, the therapeutic potential of MSC-EVs were measured in renal tubular epithelial cells injured by antimycin A. Protein sequencing analysis of hypoxic MSC-EVs was conducted, and the depletion of Glutathione S-Transferase Omega 1 (GSTO1) in hypoxic MSC-EVs was performed to verify its key role in alleviating renal injury.
Results
Hypoxia alters MSCs transcription, promotes their proliferation, and increases the production of EVs. Hypoxia-pretreated MSC-EVs exhibited a superior ability to mitigate renal IRI, enhancing proliferation and reducing apoptosis of renal tubular epithelial cells both in vivo and in vitro. Protein profiling of the EVs revealed an accumulation of numerous anti-oxidative stress proteins, with GSTO1 being particularly prominent. GSTO1 knock down was significantly reduced the antioxidant and therapeutic effects in renal IRI of hypoxic MSC-EVs.
Conclusions
Hypoxia significantly promotes MSC-EVs generation and enhances the therapeutic effect of EVs on renal IRI. The effect of antioxidant stress induced by GSTO1 is one of the most important underlying mechanisms. Our findings underscore that hypoxia-pretreated MSC-EVs represent a novel and promising therapeutic intervention for renal IRI.
Comprehensive quantitative evaluation and mechanism analysis of influencing factors on yield and quality of cultivated Gastrodia elata
Authors: Jiang Xiaojun , Dai Jing , Huang Yi , Zhao Dan , Yin Yao , Lin Qiao , Hou Mengmeng , Jin Hong 
Published: November 2024

Abstract

Gastrodia elata (G. elata) is a dual-purpose herb for medicine and food. Wild resources are depleted, and there is a significant decrease in yield or quality when they are cultivated artificially. However, what factors led to the decline is still unclear. In this study, based on comprehensive data under multiple production regions, hierarchical partitioning and partial least squares path modeling were used for the first time to quantitatively evaluate the dominant influencing factors and mechanism for the yield and quality of cultivated G. elata. The results showed that Armillaria relative abundance, soil available potassium and temperature seasonality were the key factors. Their independent effects were 74.14%, 24.78%, and 20.36% on yield, and 36.83%, 25.63%, and 21.30% on quality, respectively. Plant subspecies directly determined the yield and quality (P < 0.01). Soil physical properties affected chemical properties, which in turn affected biological properties and ultimately yields (P < 0.05). Meanwhile, soil physical properties affected quality by influencing soil chemical properties (P < 0.01). Our study contributes novel insight to optimize cultivation strategies of G. elata, providing a more comprehensive knowledge on improving the yield and quality of G. elata and achieving large-scale greenhouse cultivation.
Potential prognosisgenes in endometrial cancer: GSPT2 and CIRBP
Authors: Cai Yu , Wang Ming , He Yue , Wu Yu-mei 
Published: November 2024

Abstract

Background: The prognosis of endometrial cancer(EC) is good in the early stage, but not in the late stage and relapse stage. Its morbidity and mortality are increasing. Traditional pathological classification and risk stratification have obvious limitations. Thus, It is of great urgency to search for more prognostic molecules of endometrial cancer to develop individualized treatment strategies.
Methods: Two gene expression profiling datasets (GSE39099, GSE63678) were found from the Gene Expression Omnibus(GEO) database. The differentially expressed genes(DEGs) among EC were screened using GEO2R technique. Then these genes were analyzed by DAVID tool, STRING and Cytoscape tool to obtain the hub genes of EC prognosis. Finally, we verified them through Gepia2 platform .
Results: A total of 103 DEGs were obtained, and the top 37 hub genes of them involved the prognosis of EC, but only two (GSPT2 and CIRBP) had statistical significance: GSPT2 is related to poor prognosis of EC while CIRBP is connected with a good prognosis of EC. The expression of GSPT2 increased with the increase of clinical stage, while CIRBP decreased. And the expression of GSPT2 was higher in EC tissues as compared to normal tissues by High-throughput sequencing, and the difference was statistically significant(P < 0.05).
Conclusions: GSPT2 and CIRBP are expected to be prognostic genes for clinical diagnosis and treatment of EC.
Advancing Biodegradable Piezoelectric Polymers: Structural and Terahertz Time-Domain Spectroscopic (THz-TDS) Analysis of Crystalline Poly-L-Lactide Acid (PLLA) Films
Authors: Agarwala Shweta , Merhi Youssif , Goumarre Vincent , Amarasinghe Yasith , klarskov Pernille 
Published: November 2024

Abstract

In recent years, interest in biodegradable polymers has surged due to their diverse properties, including some remarkable characteristics such as piezoelectricity, which holds promise for next-generation sustainable electronics. This growing focus underscores the importance of understanding piezoelectric materials like Poly-L-Lactic Acid (PLLA), which requires no poling. It has been demonstrated that increasing the crystallinity in PLLA significantly enhances its piezoelectric properties, making it an ideal candidate for various biomedical applications. This paper presents an innovative, low-cost method to improve the crystallinity and crystal phase transformation of PLLA through post-processing techniques. Results indicate that the mechanical properties of the stretched PLLA films are highly dependent on the stretch ratio applied during processing. By examining how stretching influences the internal structure of PLLA, we aim to explore the material's potential for various applications and obtain a more comprehensive understanding of the piezoelectric performance. We introduce a novel approach to studying the fabricated PLLA films using Terahertz Time-Domain Spectroscopy (THz-TDS). THz-TDS revealed anisotropy and birefringence caused by the proposed post-processing technique. This was achieved by measuring changes in the absorption spectra and refractive indices as the films were rotated, providing insights into the molecular orientation. Lastly, THz-TDS was used to quantify the crystallinity of the fabricated PLLA films. The combination of THz-TDS and traditional approaches such as Wide-angle X-ray scattering (WAXS), X-ray Diffraction (XRD), Differential Scanning Calorimetry (DSC), and Fourier-Transform Infrared spectroscopy (FITR) supports our findings and better explains PLLA's behavior under various conditions. This comprehensive approach emphasizes the promise of optimizing PLLA for advanced biomedical devices.
Research on scheduling rule extraction and model comparison based on autoencoder and self-attention mechanism
Authors: pa r , Ren Pingan , Qin Hui , Mo Li , Zhang Yongchuan , Jiang Zhiqiang , Chang Xinyu , Huang Jingwei , Liu Shuai , Zhu Sipeng 
Published: November 2024

Abstract

Reservoir operation rule function is a functional mapping method that reflects specific operational rules to guide reservoir operations. With the deepening of research, the scheduling function has changed from linear to nonlinear forms, from single-objective functions to integrated multi-objective functions, from ignoring uncertainty to incorporating uncertainty analysis, from using fixed parameters to parameters that dynamically change over time, and the system has been continuously refined. Different dispatching rule functions have different actual dispatching effects (flood control, power generation benefits, water resource utilization rate and reliability). Reservoir optimal dispatching aims to identify the best rule functions to achieve the specified objectives. Through advanced computing technologies, the complex relationships between dispatch-related factors and decision variables can be extracted from large amounts of historical reservoir operation data, thus facilitating the extraction of reservoir dispatch rules. With the construction of more and more reservoirs, the hydraulic connections between reservoir groups are complex, with mutual influences occurring across both spatial and temporal scales. These complex correlation factors need to be considered when extracting dispatch rule functions. Compared to conventional operation charts, the operation rule functions extracted through data mining methods offer greater convenience in reservoir group operations. This study adopts a machine learning model based on an autoencoder and self-attention mechanism, focusing on key reservoirs along the Yangtze River and its tributaries. A combined model of these techniques is proposed, and the natural gradient boosting scheduling comparison model is applied to optimize and compare the extracted scheduling rules. The applicability of the model is verified through analysis, and the results are further explained using SHAP (SHapley Additive exPlanations) theory, which provides interpretability for the model results.
Effect of Intraoperative Positioning on Lavage Efficacy and Adverse Events in Whole Lung Lavage: A Retrospective Study
Authors: Gao Ruiyue , Gu Wenjie , Li Hongjie , Chu Huiwen , Tong Zhaohui , Wang Zhen 
Published: November 2024

Abstract

Background
This study aimed to compare the effects of different intraoperative positions on the Lavage efficacy and adverse outcomes of whole lung lavage (WLL).
Methods
Patients who underwent whole lung lavage surgery at Beijing Chaoyang Hospital between 2016 and 2023 were included in the study. Patient demographic information was collected and summarized. Patients were divided into two groups based on their intraoperative position, supine or lateral. Baseline characteristics, and lavage efficacy and the occurrence of adverse reactions after surgery were compared between the two groups.
Results
Forty-five procedures of WLL were performed at Beijing Chaoyang Hospital between 2016 and 2023 in 22 patients with an average age of 43 years, with the male : female ratio was 13:9. Among them, 26 and 18 patients were placed in the supine and 18 cases in the lateral position, respectively. There was no significant difference in the volume instilled between the two position groups (10,000 [10,000–11,250] ml vs. 10,000 [6,375–13,000] ml, P = 0.78) or in the volume drained (11,000 [9,950–12,650] ml vs. 10,060 [6,175–13,250] ml, P = 0.324). However, the lavage balance in the prone position group was greater than in the lateral position group (-739.23 ± 735.59 ml vs. 1.11 ± 887.08 ml, P = 0.004), and the lavage rate showed a significant difference (1.08 [1.03, 1.10] vs. 1.00 [0.94, 1.05], P = 0.003). Neither group experienced serious complications and no significant differences in the postoperative adverse events between the two groups in terms of hypoxemia, fluid extravasation, pleural effusion, fever, wheezing, prolonged intubation, respiratory acidosis, and transient cerebral ischemia.
Conclusion
Compared to the lateral position, the supine position had higher efficacy in whole lung lavage. There was no significant difference in postoperative adverse reactions between the two positions.
Trial registration:
Retrospectively registered.
Navigating the Challanges: Strategies of Migrant Workers in Turkey to Cope With Workplace Bullying
Authors: uyan umut , Ibin Adil 
Published: November 2024

Abstract

Despite being discussed by the scientific community and practitioners for decades; bullying remains a severe problem in modern work life. The problem will likely continue to grow exponentially with the reality of migration. In fact, immigrant workers have been reported to be in the highest risk group in terms of being exposed to workplace bullying. The aim of the study is to understand the extent to which migrant textile workers in Turkey are exposed to bullying behaviors and the strategies they develop to cope with these unintended behaviors. The data of the research, which has a qualitative design, was collected through in-depth interviews (N = 24) and analyzed thematically. The study demonstrates that migrant workers utilize a range of strategies to handle workplace bullying, which can be classified into three primary categories: Enhancing work-related competencies, eliminative and preventive measures. Enhancing professional skills and expanding work-related networks are central to building resilience, while preventive measures include forging alliances with native counterparts and choosing supportive work environments. Eliminative strategies such as mental support, quiet quitting, and alternative employment options underscore the multifaceted nature of coping mechanisms among migrant workers.
Nutritional Optimization of Muffins using Wheat, Bean and Quinoa Flour: A Strategy for Enhancing Protein Quality and Healthy Benefits
Authors: Bassett María Natalia , Acuña María Elina , Corral Lucrecia , Moreno Elvecia Carmen , Fernández Noelia Natalia , Rossi Analia Mabel , Marigliano Ana Clelia Gómez , Tereschuk María Laura 
Published: November 2024

Abstract

The objective of the present study was to elaborate muffins optimizing nutritional quality using protein complementation, with regionally produced underutilized ingredients. The theoretical protein quality of various mixtures was assessed using the "digestible essential amino acid score" (DIAAS) method, using the FAO standard requirements as a reference protein. A muffin was subsequently designed, formulated, and produced using the optimal ratio of wheat, bean, and quinoa flour mixture, and compared to a control made with 100% wheat flour. The proteins, fats, dietary fibre, ashes, and moisture were determined using AOAC methods. Height, texture, and colour were evaluated using a Vernier calliper, a TA-XT 2 plus Texturometer, and a Colour Quest XE spectrophotometer. Antioxidant activity was determined using the DPPH radical decolouration method, and total phenolic compounds were quantified using the Folin-Ciocalteu method. The sensory characteristics and global acceptability were also performed. Results showed that the moisture, protein, fat, fibre content, hardness, colour and the total phenol content (p < 0,05) of muffins are significantly different from each other. The partial replacement of wheat flour with quinoa and bean flours caused a decrease in muffin height while increasing hardness, total phenol content, protein and dietary fibre content. The elaborated muffin showed good sensory acceptance, scoring 6.4 on a 9-point hedonic scale, and achieved an acceptability index of 71%. Formulating this muffin with a good nutritional balance offers an effective way to incorporate beans and quinoa into regular diets, helping to prevent chronic non-communicable and deficiency diseases in vulnerable populations.