bluuebunny/crossref_metadata_2025_split huggingface dataset:
Divergence in Thermostability of Arabidopsis Mitochondrial Nucleotide Exchange Factors Encoded by Duplicate Genes, MGE1 and MGE2
Authors: Chen Zih-teng , Hung Meng-Ju , Yu Shih-Jiun , Liao Tai-Yan , Lin Yao-Pin , Chen Rita P.-Y. , Yang Chien-Chih , Charng Yee-yung 
Published: February 2019

ABSTRACTThe divergence of duplicate genes links to organismic adaptation. In Arabidopsis thaliana two nuclear genes encode mitochondrial GrpEs, MGE1 and MGE2, the nucleotide exchange factors of DnaK/HSP70 chaperone. MGE1 and MGE2 are duplicate genes originated from a whole genome duplication event. They respond differentially to high temperature; MGE2 is heat-inducible and is required for Arabidopsis seedlings to tolerate prolonged heat stress, while MGE1 is constitutively expressed. Heterologous expression of MGE2 but not MGE1 restored the growth of E. coli grpE mutant cells at elevated temperatures, suggesting that MGE2 is more thermostable than MGE1. In this study, we directly compared the thermostability of the purified recombinant MGE1 and MGE2 by circular dichroism spectroscopy. The temperature midpoints of the unfolding transition (Tm) of MGE1 and MGE2 were about 38 and 46 °C, respectively, indicating that MGE2 is remarkably more stable than MGE1 at higher temperature. Domain swapping between the two homologous proteins showed that the N-terminal region, including an unstructured sequence and a long α-helix domain, is the major determinant of the thermostability. Although MGE2 contains a conserved sequence derived from an exonized intron within the N-terminus unstructured region, deletion of this sequence did not substantially affect protein thermostability in vitro and complementation of E. coli and Arabidopsis heat sensitive mutants. Taken together, our results suggest that Arabidopsis MGE1 and MGE2 had diverged not only in transcriptional response but also in the thermostability of the encoded proteins, which may contribute to adaptation of plants to higher temperatures.
The yeast Art1 arrestin domain contains disordered insertions that regulate its localization and activity
Authors: Baile Matthew G. , Guiney Evan L. , Sanford Ethan J. , MacGurn Jason A. , Smolka Marcus B. , Emr Scott D. 
Published: February 2019

ABSTRACTThe protein composition of the plasma membrane is rapidly remodeled in response to changes in nutrient availability or cellular stress. This occurs, in part, through the selective ubiquitylation and endocytosis of plasma membrane proteins which, in the yeastSaccharomyces cerevisiae,is mediated by the HECT E3 ubiquitin ligase Rsp5 and arrestin-related trafficking (ART) adaptors. Here, we provide evidence that an ART family member, Art1, consists of an arrestin fold with extended N- and C-terminal tails, and interspersed with loop insertions. These loop and tail regions, while not strictly required for Art1 function, regulate its activity through two separate mechanisms. One loop mediates Art1 cargo specificity. Other loops are subjected to phosphorylation in a manner dependent on the Pho85 cyclins Clg1 and Pho80. Phosphorylation of the loops controls Art1’s localization to the plasma membrane, which promotes cargo ubiquitylation and endocytosis, demonstrating a mechanism through which Art1 activity is regulated.
The down regulation of megalin/LRP2 by transforming growth factor beta (TGF-β1) is mediated by the SMAD2/3 signalling pathway
Authors: Cabezas Felipe , Farfán Pamela , Marzolo María-Paz 
Published: February 2019

AbstractMegalin/LRP2 is a receptor that plays important roles in the physiology of several organs, such as kidney, lung, intestine, and gallbladder; and also in the physiology of the nervous system. Megalin expression is reduced in diseases associated with fibrosis, including diabetic nephropathy, hepatic fibrosis and cholelithiasis, as well as in some breast and prostate cancers. One of the hallmarks of these conditions is the presence of the cytokine transforming growth factor beta (TGF-ß). Although TGF-ß has been implicated in the reduction of megalin levels, the molecular mechanism underlying this regulation is not well understood. Here, we show that treatment of two epithelial cell lines (from kidney and gallbladder) with TGF-ß1 is associated with decreased megalin mRNA and protein levels, and that these effects are reversed by inhibiting the TGF-ß1 type I receptor (TGF-ßRI). Based onin silicoanalyses, the two SMAD-binding elements (SBEs) in the megalin promoter are located at positions −57 and −605. Site-directed mutagenesis of the SBEs and chromatin immunoprecipitation (ChIP) experiments revealed that SMAD2/3 transcription factors interact with SBEs to repress the megalin promoter and that they are also required for the repressing role of TGF-ß1. In addition, high concentration of albumin reduced megalin expression and promoter activation that depend on the expression of SMAD2/3. Interestingly, the histone deacetylase inhibitor Trichostatin A (TSA), which induces megalin expression, reduced the effects of TGF-ß1on megalin mRNA levels. These data show the significance of TGF-ß and the SMAD2/3 signalling pathway in the regulation of megalin and explain the decreased megalin levels observed under conditions in which TGF-ß is upregulated, including fibrosis-associated diseases and cancer.
The foveal visual representation of the primate superior colliculus
Authors: Chen Chih-Yang , Hoffmann Klaus-Peter , Distler Claudia , Hafed Ziad M. 
Published: February 2019

AbstractProcessing of foveal retinal input is important not only for high quality visual scene analysis, but also for ensuring precise, albeit tiny, gaze shifts during high acuity visual tasks. The representations of foveal retinal input in primate lateral geniculate nucleus and early visual cortices have been characterized. However, how such representations translate into precise eye movements remains unclear. Here we document functional and structural properties of the foveal visual representation of midbrain superior colliculus. We show that superior colliculus, classically associated with extra-foveal spatial representations needed for gaze shifts, is highly sensitive to visual input impinging on the fovea. Superior colliculus also represents such input in an orderly and very specific manner, and it magnifies representation of foveal images in neural tissue as much as primary visual cortex does. Primate superior colliculus contains a high-fidelity visual representation, with large foveal magnification, perfectly suited for active visuomotor control and perception.
Assessment of a reconfigurable manufacturing system
Authors: Prasad Durga , Jayswal S.C. 
Published: February 2019

PurposeThe purpose of this paper is to develop the methodology which can facilitate the concept of reconfiguration in the manufacturing system.Design/methodology/approachDesign methodology includes the calculation of similarity matrix, formation of part family, and selection of part family. ALC algorithm has been used for part family formation and three criteria have been considered for the selection of part family. These criteria are reconfiguration effort, under-utilization cost, and floor space cost. AHP has been used to calculate the weights of criteria and reference ideal method has been used for the selection of alternatives.FindingsIn the manufacturing system, machines should be grouped on the basis of reconfiguration cost. When the time period is less, light machines and Group 1 machines are added and removed. In the case study, the concept of reconfiguration is useful for families (A, B, C, D). Machines can be reused by adding/removing some modules of machines. The concept of reconfiguration becomes more useful when it is implemented with lean manufacturing. Lean manufacturing techniques Jidoka and Poka-yoke are used to increase the diagnosability of the system.Practical implicationsIndustrial case study has been considered.Social implicationsMarket competition is increasing rapidly and it increases the demand and variety of products, due to which manufacturing enterprises are forced to adapt a manufacturing system which can adjust its capacity and functionality quickly at low cost. To reconfigure manufacturing system from one product/product family to another product/product family, changes can be done in hardware and/or software components in response to sudden changes in the market or in regulatory requirements.Originality/valueAn integrated approach for reconfiguration has been proposed considering the industrial application. It includes weighted Jaccard function, ALCA, AHP, RIM. The methodology for calculation of reconfiguration effort, under-utilization cost, and floor space cost has been presented for industrial case.
Barriers to BIM implementation strategies in China
Authors: Zhou Yijun , Yang Yu , Yang Jyh-Bin 
Published: February 2019

Purpose
Effective BIM application hinges on the development of appropriate strategies for its implementation. Though some strategies have been deployed to facilitate BIM implementation in China, their outcomes are not clear. The purpose of this paper is to provide recommendations regarding appropriate strategies for promoting the development and implementation of BIM in China based on lessons learned from advanced implementation experiences in other countries.

Design/methodology/approach
First, existing strategies are investigated and barriers to BIM implementation mentioned in previous studies are summarized. Then, the identified barriers are mapped to the strategy contents. Finally, a comparative analysis on different areas is conducted to propose suggestions for identified items of BIM implementation strategies that need to be improved.

Findings
Six unaddressed barriers to BIM implementation strategies in China were identified from the mapping results: insufficient government lead/direction, organizational issues, legal issues, high cost of application, resistance to change of thinking mode and insufficient external motivation.

Originality/value
The findings of this study can be used to facilitate the development of appropriate strategies within the public and private sectors for promoting BIM implementation in China and elsewhere.
Research on mutual relationships of flange ring and traveler on ring spinning system
Authors: Su Xuzhong , Liu Xinjin , Li Siying 
Published: February 2019

PurposeRing spinning is the most widely used spinning method at present. In the spinning, ring and traveller are the two important components of the twisting process. The properties of ring and traveller have a direct relationship with the yarn qualities and spinning speed. Therefore, the purpose of this paper is to study the mutual relationships of flange ring and traveller system by taking the 6802-type traveller with rectangular structure, BU-type traveller with toxoplasma structure and 772-type traveller with corrugated structure as examples.Design/methodology/approachFirst, the theoretical calculation models of force and inclinations of the traveller are given, and calculation methods of corresponding key parameters referred in the models are presented. Then, by using SolidWorks software, models of the three kinds of traveller straddling on the PG1-4254 type ring are built, and the traveller and ring are simulated by using ADAMS software. By using MATLAB software, the force and inclinations of the traveller during the rotation around ring are simulated. Finally, the spinning experiments are made.FindingsIt is shown that the abrasion of 6802-type traveller is the most serious, and corresponding yarn evenness and hairiness is the worst. The abrasion of BU-type traveller is the slightest, and corresponding yarn evenness is the best. The yarn spins by using 772-type traveller has the least hairiness.Originality/valueIn the paper, the mutual relationships of flange ring and traveller system have been studied. For giving analysis of force and inclinations of the traveller, calculation methods of the key common parameters are presented. By using SolidWorks software, the physical models of the three kinds of traveller straddling on the PG1-4254 type ring are built. And then, the force and inclinations of the traveller during the rotation around ring are simulated by using MATLAB software. At last, the spinning experiments are made to analyse the simulation results.
Quick response code scanning for children’s informal learning
Authors: Chung Tingting , Wilsey Stephanie , Mykita Alexandra , Lesgold Elaine , Bourne Jennifer 
Published: February 2019

Purpose
Mobile technologies, such as QR codes, play a particularly important role in scaffolding the child user’s active learning in informal environments. The purpose of this paper is to examine the impact of QR code scanning on two informal learning outcomes: increased interest and greater knowledge understanding.

Design/methodology/approach
In total, 91 children and their families participated in the study as part of the iQ Zoo Project. Children in both the smartphone group and the control group completed were assessed qualitatively and quantitatively before and then after their zoo visits.

Findings
Qualitative findings suggest that most children’s interest in learning about animals was sustained as a result of the experience. Quantitative results reveal that QR code scanning was effective in promoting knowledge gains, especially on subjects that are challenging for the informal learner. Findings were comparable across the younger (5–8) and older (9–12) age groups.

Originality/value
This study provides empirical support for the value and usefulness of mobile technologies such as QR code scanning for children's learning in informal environments.
Pressures of CSR in India: an institutional perspective
Authors: Aggarwal Vijita S. , Jha Aruna 
Published: February 2019

PurposeWide differences in the focus and form of corporate social responsibility (CSR) exist among countries due to the different institutional embeddedness of CSR practices. The purpose of this paper is to seek to explain them within the framework provided by institutional theory by identifying important pressures driving CSR practices. Further, it intends to extend theory by proposing a conceptual model that relates institutional pressures, CSR practices, reputation and financial performance of corporates in a developing country like India.Design/methodology/approachDrawing upon the extant literature on the constructs, the paper describes their evolution through decades and weaves relationship between them. Institutional theory provides the framework to develop hypotheses.FindingsThe model has its roots in Scott’s institutional theory – linking regulative, normative and cognitive pressures to CSR practices. Reputation mediates the relationship between CSR and financial performance.Practical implicationsThe conceptual model can serve as a foundation for subsequent empirical research. An understanding of relationship between constructs in the model will help corporates to strategize CSR initiatives. At the organisational level, insight into managerial perceptions of CSR practices will help to identify the need for training, if there is a gap between what organisation intends and what managers perceive.Originality/valueThe authors have proposed for the first time an integrative model that will help to understand the antecedents as well as consequences of CSR practices in a developing country within a theoretical framework.
Learning to read online: the effect of instruction on e-textbook use
Authors: Ragan Amy , Kammer Jenna , Atkins Charlene , Burress Rene 
Published: February 2019

PurposeThe purpose of this paper is to examine the effect of library instruction on the use of e-textbook features in a seventh-grade science class in Budapest, Hungary. Using the theory of value-expectancy, library instruction was designed to show students how the e-textbook features would improve their study habits.Design/methodology/approachUsing a time-series, within-subject design, the researchers examined the students’ use of e-textbooks before receiving library instruction, and then again after receiving library instruction. Data were collected from student survey responses, focus group interviews, and digital library usage. A repeated-measurest-test was used to compare data collected prior to and following the instructional sessions.FindingsThe results indicate that the use of e-textbook features (glossary, audio, quizzes, notes, highlighter, and video) increased after library instruction. While the use of e-textbook features increased, this did not translate to other types of e-books: the use of the digital library did not increase.Research limitations/implicationsThis paper has implications for research on the use of e-textbooks in academic settings. Baseline findings support the existing literature that shows that students do not use all of the features of an e-textbook. The research in this study adds that direct instruction on those features will increase use.Practical implicationsLibrarians and teachers may want to consider direct instruction on e-textbooks. While it may not increase digital library usage, it may benefit the student learning experience.Originality/valueThis study builds on the work related to the student experience of using e-textbook. It highlights the value of library instruction in improving the student experience and use of e-textbooks.
Evaluating national library mission statements in Ibero-America
Authors: Pacios Ana Reyes , Pérez-Piriz María Pilar 
Published: February 2019

Purpose
The purpose of this paper is to review the websites of 22 national libraries in Ibero-America to determine whether and how effectively they display these organisations’ mission statements, as well as any convergence/divergence among these texts.

Design/methodology/approach
A review was conducted of the national library websites of ABINIA’s 22 members to locate their respective mission statements. The statements identified were analysed and evaluated against the positioning and presence criteria and wording proposed by experts.

Findings
Website content clearly attests to national libraries’ eagerness to publicise their mission statements, which are readily accessible in most cases. Their functions are represented to a more or less standard pattern. Most are portrayed as institutions responsible for custodying, enriching, preserving and disseminating their countries’ cultural legacy. Other purposes mentioned include the promulgation of and accessibility to the heritage custodied.

Practical implications
The paper may prove useful for professional librarians involved in drafting or revising their organisation’s mission statement in the wake of changing circumstances or on the occasion of the formulation of a new strategic plan.

Originality/value
Of the very short number of analyses of libraries’ mission statements published to date, none discusses national libraries. This is the very first study of national library mission statement in Ibero-America. It forms part of a line of research dealing with national library mission statements defined and available on institutional websites for countries anywhere in the world.
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Authors: Markowitz-Elfassi Dana , Yarchi Moran , Samuel-Azran Tal 
Published: February 2019

Purpose
The purpose of this paper is to examine the effect of politicians’ facial attractiveness on their online popularity as reflected in audience engagement with their Facebook posts during the 2015 Israeli election campaign.

Design/methodology/approach
Using Israel’s 2015 election campaign as the case study, the authors analyzed all messages posted (n=501) on 33 politicians’ official Facebook pages during the week leading to Election Day.

Findings
The results demonstrate that audiences do engage more with posts of the more facially attractive politicians. These posts generated more shares, more comments and more participants in their discussions – but not more likes – relative to posts of less attractive politicians. These effects became even stronger when the posts were accompanied by one or more visual image, and remained significant even after controlling for other engagement predictors, such as a politician’s gender, seniority or the timing of a post’s publication.

Social implications
The findings emphasize the importance of attractive looks for politicians. The findings highlight that attractive politicians’ posts attract more attention, allowing them to better spread their ideas. Thus, politicians should aim to post aesthetic images and visuals to promote better engagement with their ideas on social media.

Originality/value
The study expands our understanding of online presentations of politicians, focusing on the effect of politicians’ facial attractiveness on their online popularity. Recent studies have demonstrated that physically attractive politicians enjoy more and better media attention on television news, but not in non-visual media such as radio and newspapers. This effect has not been examined in the social media environment, a central arena for today’s political debates and one that involves many visual messages.
Properties of soft magnetic Fe-Co-V alloy produced by laser powder bed fusion
Authors: Riipinen Tuomas , Metsä-Kortelainen Sini , Lindroos Tomi , Keränen Janne Sami , Manninen Aino , Pippuri-Mäkeläinen Jenni 
Published: February 2019

Purpose
The purpose of this paper is to report on the developments in manufacturing soft magnetic materials using laser powder bed fusion (L-PBF).

Design/methodology/approach
Ternary soft magnetic Fe-49Co-2V powder was produced by gas atomization and used in an L-PBF machine to produce samples for material characterization. The L-PBF process parameters were optimized for the material, using a design of experiments approach. The printed samples were exposed to different heat treatment cycles to improve the magnetic properties. The magnetic properties were measured with quasi-static direct current and alternating current measurements at different frequencies and magnetic flux densities. The mechanical properties were characterized with tensile tests. Electrical resistivity of the material was measured.

Findings
The optimized L-PBF process parameters resulted in very low porosity. The magnetic properties improved greatly after the heat treatments because of changes in microstructure. Based on the quasi-static DC measurement results, one of the heat treatment cycles led to magnetic saturation, permeability and coercivity values comparable to a commercial Fe-Co-V alloy. The other heat treatments resulted in abnormal grain growth and poor magnetic performance. The AC measurement results showed that the magnetic losses were relatively high in the samples owing to formation of eddy currents.

Research limitations/implications
The influence of L-PBF process parameters on the microstructure was not investigated; hence, understanding the relationship between process parameters, heat treatments and magnetic properties would require more research.

Originality/value
The relationship between microstructure, chemical composition, heat treatments, resistivity and magnetic/mechanical properties of L-PBF processed Fe-Co-V alloy has not been reported previously.