bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pseudomonas spp. are key players in agricultural biogas substrate degradation
Authors: Buettner Christian , von Bergen Martin , Jehmlich Nico , Noll Matthias 
Published: September 2019

AbstractAnaerobic degradation (AD) of heterogeneous agricultural substrates is a complex process involving a diverse microbial community. While microbial community composition of a variety of biogas plants (BPs) is well described, little is known about metabolic processes and microbial interaction patterns. Here, we analyzed 16 large-scale BPs using metaproteomics. All metabolic steps of AD were observed in the metaproteome, and multivariate analyses indicated that they were shaped by temperature, pH, volatile fatty acid content and substrate types. Biogas plants could be subdivided into hydrogenotrophic, acetoclastic or a mixture of both methanogenic pathways based on their process parameters, taxonomic and functional metaproteome. Network analyses showed large differences in metabolic and microbial interaction patterns. Both, number of interactions and interaction partners were highly dependent on the prevalent methanogenic pathway for most species. Nevertheless, we observed a highly conserved metabolism of different abundantPseudomonas spp. for all BPs indicating a key role during AD in carbohydrate hydrolysis irrespectively of variabilities in substrate input and process parameters. Thus,Pseudomonas spp. are of high importance for robust and versatile AD food webs, which highlight a large variety of downstream metabolic processes for their respective methanogenic pathways.
Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane
Authors: Marsit Nagi M. , Sidney Laura E. , Britchford Emily R. , McIntosh Owen D. , Allen Claire L. , Ashraf Waheed , Bayston Roger , Hopkinson Andrew 
Published: September 2019

AbstractAmniotic membrane (AM) is used to treat a range of ophthalmic indications but must be presented in a non-contaminated state. AM from elective caesarean sections contains natural microbial contamination, requiring removal during processing protocols. The aim of this study was to assess the ability of antibiotic decontamination of AM, during processing by innovative low-temperature vacuum-drying. Bioburden of caesarean section AM was assessed, and found to be present in low levels. Subsequently, the process for producing vacuum-dried AM (VDAM) was assessed for decontamination ability, by artificially loading with Staphylococcus epidermidis at different stages of processing. The protocol was highly efficient at removing bioburden introduced at any stage of processing, with antibiotic treatment and drying the most efficacious steps. The antibacterial activity of non-antibiotic treated AM compared to VDAM was evaluated using minimum inhibitory/biocidal concentrations (MIC/MBC), and disc diffusion assays against Meticillin-resistant Staphylococcus aureus, Meticillin-resistant S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Enterococcus faecalis. Antibacterial activity without antibiotic was low, confirmed by high MIC/MBC, and a no inhibition on agar lawns. However, VDAM with antibiotic demonstrated effective antibacterial capacity against all bacteria. Therefore, antibiotic decontamination is a reliable method for sterilisation of AM and the resultant antibiotic reservoir is effective against gram-positive and –negative bacteria.
Gestures convey different physiological responses when performed toward and away from the body
Authors: Bartolo Angela , Claisse Caroline , Gallo Fabrizia , Ott Laurent , Sampaio Adriana , Nandrino Jean-Louis 
Published: September 2019

AbstractWe assessed the sympathetic and parasympathetic activation associated to the observation of Pantomime (i.e. the mime of the use of a tool) and Intransitive gestures (i.e. expressive) performed toward (e.g. a comb and “thinking”) and away from the body (e.g. key and “come here”) in a group of healthy participants while both pupil dilation (N = 31) and heart rate variability (N = 33; HF-HRV) were recorded. Large pupil dilation was observed in both Pantomime and Intransitive gestures toward the body; whereas an increase of the vagal suppression was observed in Intransitive gestures away from the body but not in those toward the body. Our results suggest that the space where people act when performing a gesture has an impact on the physiological responses of the observer in relation to the type of social communicative information that the gesture direction conveys, from a more intimate (toward the body) to a more interactive one (away from the body).
Clinical Model for Predicting Warfarin Sensitivity
Authors: Ma Zhiyuan , Cheng Gang , Wang Ping , Khalighi Bahar , Khalighi Koroush 
Published: September 2019

AbstractWarfarin is a widely used anticoagulant with a narrow therapeutic index and large interpatient variability in the therapeutic dose. Complications from inappropriate warfarin dosing are one of the most common reasons for emergency room visits. Approximately one third of warfarin dose variability results from common genetic variants. Therefore, it is very necessary to recognize warfarin sensitivity in individuals caused by genetic variants. Based on combined polymorphisms in CYP2C9 and VKORC1, we established a clinical classification for warfarin sensitivity. In the International Warfarin Pharmacogenetic Consortium (IWPC) with 5542 patients, we found that 95.1% of the Black in the IWPC cohort were normal warfarin responders, while 74.8% of the Asian were warfarin sensitive (P < 0.001). Moreover, we created a clinical algorithm to predict warfarin sensitivity in individual patients using logistic regression. Compared to a fixed-dose approach, the clinical algorithm provided significantly better performance. In addition, we validated the derived clinical algorithm using the external Easton cohort with 106 chronic warfarin users. The AUC was 0.836 vs. 0.867 for the Easton cohort and the IWPC cohort, respectively. With the use of this algorithm, it is very likely to facilitate patient care regarding warfarin therapy, thereby improving clinical outcomes.
Characterization of Microbial Communities, Identification of Cr(VI) Reducing Bacteria in Constructed Wetland and Cr(VI) Removal Ability of Bacillus cereus
Authors: Lin Hua , You Shaohong , Liu Liheng 
Published: September 2019

AbstractIn this study, the contribution of substrates microorganisms in three different constructed wetlands (CWs) to Cr(VI) purification was discussed. In addition, the microbial communities in the substrate of different CWs were characterized, and rhizosphere Cr(VI) reducing bacteria was also identified. The results showed that microorganisms could improved Cr(VI) removal to 76.5%, and result in that more Cr(VI) was reduced to Cr(III). The dominant strains in the substrates of different CWs were Sphingomonas sp., Cystobacter sp., Acidobacteria bacterium, Sporotrichum and Pellicularia species. The Cr(VI) reducing bacteria from Leersia hexandra Swartz rhizosphere was identified as Bacillus cereus. Furthermore, under suitable conditions, the removal rate of Cr(VI) by Bacillus cereus was close to 100%.
Visual impairment in high flow and low flow carotid cavernous fistula
Authors: Alam Md. Shahid , Jain Mukesh , Mukherjee Bipasha , Sharma Tarun , Halbe Swatee , Jaisankar Durgasri , Raman Rajiv 
Published: September 2019

AbstractOur aim is to study the varied posterior segment manifestations, level of visual impairment (VI) and its causes in carotid cavernous fistula (CCF) patients. A retrospective study was done, wherein data was obtained from 48 digital subtraction angiogram (DSA) proven CCF patients. CCF was classified according to Barrow et al., based on DSA into type A (high flow) and types B, C and D (low flow). High flow CCF was present in 8 (16.7%) and low flow CCF was present in 42 (83.3%). Compared to low flow group, patients in high flow group were younger and had a history of trauma (p < 0.05). Posterior segment findings ranged from familiar stasis retinopathy and optic neuropathy (both, glaucomatous and ischemic) to uncommon findings of central retinal artery occlusion, Terson syndrome and combined retinal and choroidal detachment. Retinal vein dilatation was the most common finding in both groups. The high flow CCF group had 6 (75%) patients that had VI. This was acute in 4 (50%) patients and delayed in 2 (25%). In the low flow group 10 (23.8%) of patients had delayed VI. The identification of “3 point sign” is a novel finding of this study, not described before. While none of three findings (disc hyperaemia, retinal vein dilatation and intra-retinal haemorrhage) in isolation were predictive of visual loss, but when present together results in visual loss. Posterior segment changes were varied, some are uncommon and can occur in various combinations. “3 point sign” must be identified at the earliest to prevent visual impairment. The incidence of VI in CCF patients is high.
Effects of anti-H. pylori triple therapy and a probiotic complex on intestinal microbiota in duodenal ulcer
Authors: Wu Lili , Wang Zikai , Sun Gang , Peng Lihua , Lu Zhongsheng , Yan Bin , Huang Kun , Yang Yunsheng 
Published: September 2019

AbstractThis study aimed to investigate the intestinal microbiota in duodenal ulcer (DU) patients, effects of proton pump inhibitors,clarithromycin and amoxicillin, PCA) for Helicobacter pylori (H. pylori) and Bacillus subtilis and Enterococcus faecium (BSEF) on intestinal microbiota. DU patients were randomly assigned to receive either PCA (group TT) or PCA plus BSEF(group TP). The fecal microbiome was conducted using high throughput 16S rDNA gene and internal transcribed spacer sequencings. The diversity and abundance of intestinal bacteria in the DU were significantly lower than health check control (HC) group. In the TT group, the abundance and diversity of both intestinal bacteria and fungi decreased after PCA treatment, compared with those before treatment, whereas in the TP group no obvious changes were observed. In the TT group at all the time points, both the intestinal bacteria and fungi were different from those in the HC group. However, in the TP group, at 10w the bacterial flora abundance was close to that in the HC group. The results indicate that anti- H. pylori treatment induced significant decrease in the diversity of intestinal microbiota, while the combined therapy supplemented with BSEF could protect and restore the intestinal microbiota.
Toward clinical digital phenotyping: a timely opportunity to consider purpose, quality, and safety
Authors: Huckvale Kit , Venkatesh Svetha , Christensen Helen 
Published: September 2019

AbstractThe use of data generated passively by personal electronic devices, such as smartphones, to measure human function in health and disease has generated significant research interest. Particularly in psychiatry, objective, continuous quantitation using patients’ own devices may result in clinically useful markers that can be used to refine diagnostic processes, tailor treatment choices, improve condition monitoring for actionable outcomes, such as early signs of relapse, and develop new intervention models. If a principal goal for digital phenotyping is clinical improvement, research needs to attend now to factors that will help or hinder future clinical adoption. We identify four opportunities for research directed toward this goal: exploring intermediate outcomes and underlying disease mechanisms; focusing on purposes that are likely to be used in clinical practice; anticipating quality and safety barriers to adoption; and exploring the potential for digital personalized medicine arising from the integration of digital phenotyping and digital interventions. Clinical relevance also means explicitly addressing consumer needs, preferences, and acceptability as the ultimate users of digital phenotyping interventions. There is a risk that, without such considerations, the potential benefits of digital phenotyping are delayed or not realized because approaches that are feasible for application in healthcare, and the evidence required to support clinical commissioning, are not developed. Practical steps to accelerate this research agenda include the further development of digital phenotyping technology platforms focusing on scalability and equity, establishing shared data repositories and common data standards, and fostering multidisciplinary collaborations between clinical stakeholders (including patients), computer scientists, and researchers.
A polyrotaxanated covalent organic network based on viologen and cucurbit[7]uril
Authors: Das Gobinda , Sharma Sudhir Kumar , Prakasam Thirumurugan , Gándara Felipe , Mathew Renny , Alkhatib Nour , Saleh Na’il , Pasricha Renu , Olsen John-Carl , Baias Maria , Kirmizialtin Serdal , Jagannathan Ramesh , Trabolsi Ali 
Published: September 2019

AbstractSince their initial presentation in 2005 by Yaghi and co-workers, covalent organic frameworks (COFs) have been the focus of much development. Here we introduce mechanical bonds into COFs by employing a method that involves supramolecular self-assembly, dynamic imine-bond formation, and liquid-liquid interfacial condensation and produces a continuous 2D polyrotaxaneted film. A diamino-viologen linker is complexed with the macrocycle cucurbit[7]uril (CB[7]) to form pseudorotaxanes that then condense with an aromatic tri-aldehyde core at the interface of two solvents. The unrotaxanated COF is prepared by condensing the viologen and the trialdehyde in the absence of CB[7], and the chemical and physical properties of the formed films are compared. The CB[7]–encapsulated film is mechanically and thermally more robust and more luminescent. The synthetic strategy presented here allows the incorporation of mechanically interlocked molecules (MIMs) into polymeric materials and provides access to materials that have properties and functionalities that are yet to be explored.
Copper(ii) complexes with alloferon analogues containing phenylalanine H6F and H12F stability and biological activity lower stabilization of complexes compared to analogues containing tryptophan
Authors: Kowalik-Jankowska Teresa , Lesiów Monika , Krupa Katarzyna , Kuczer Mariola , Czarniewska Elżbieta 
Published: September 2019

AbstractCopper(ii) complex formation processes between alloferon 1 (Allo1) (H1 GVSGH6 GQH9 GVH12G) analogues where the phenylalanine residue is introduced in the place of His residue H6F and H12F have been studied by potentiometric, UV-visible, CD and EPR spectroscopic, and MS methods. For the phenylalanine analogues of alloferon 1, complex speciation has been obtained for a 1 : 1, 2 : 1 and 3 : 1 metal-to-ligand molar ratio. At physiological pH and in 1 : 1 metal-to-ligand molar ratio the phenylalanine analogues of alloferon 1 form a CuL complex similar to that of alanine analogues with the 4N{NH2,Nlm1,2Nlm} coordination mode. The stability of the complexes of the phenylalanine analogues is higher in comparison to those of alanine analogues, but lower in comparison to those containing tryptophan. Injection of Allo12F into insects induced prominent apoptotic changes in all hemocytes. The presence of apoptotic bodies only in the insect hemolymph testifies to the fact that Allo12F is an extremely pro-apoptotic peptide.
Perianal Crohn's Disease
Authors: Pogacnik Javier Salgado , Salgado Gervasio 
Published: September 2019

AbstractThis article provides an overview of the principles in the evaluation and management of perianal Crohn's disease (CD). Manifestation-specific treatment is addressed including abscess, fistula, skin tags, hemorrhoids, fissure, ulcers, strictures, ano-, and rectovaginal fistulas as well CD-associated hidradenitis suppurativa.
Invasive Candida Infections in a Pediatric Intensive Care Unit in Turkey: Evaluation of an 11-Year Period
Authors: Aslan Nagehan , Yildizdas Dincer , Alabaz Derya , Horoz Ozden Ozgur , Yontem Ahmet , Kocabas Emine 
Published: September 2019

AbstractThe aim of this study was to evaluate the Candida species, predisposing factors, antifungal treatment approaches, and clinical outcomes of invasive Candida infections (ICIs) in a tertiary pediatric intensive care unit (PICU). A retrospective study was performed from January 2008 to January 2019 including 102 children with ICIs who were admitted to a university hospital in southeastern Turkey. Positive blood cultures were detected in 43 (42.1%) patients, and positive urine cultures were detected in 59 (57.8%). According to our results, Candida albicans (42.2%) was the most common species for all isolates followed by C. parapsilosis (17.6%). In our patient population, non-albicans Candida species were dominant (57.8%) in all isolates. The most common facilitating factor in our study was the use of mechanical ventilator support (87.3%). The mortality rate of our patients with ICIs was 13.7%. Candida albicans was found to have the highest mortality rate among all Candida species (30.7%). When we compared patients with C. albicans and those with non-albicans Candida species in terms of risk factors, we detected a significant difference between the two groups for total parenteral nutrition use (p = 0.027). Fluconazole was the most preferred (58.8%) treatment option in our PICU for ICIs. Our results showed an increased trend in micafungin use in recent years. ICIs are a significant problem due to the high mortality and morbidity rates in critically ill pediatric patients in PICUs. In recent years, an increase in Candida infections caused by non-albicans Candida species has been reported. Multicenter prospective studies are needed to determine the risk factors for ICIs.
Effect of the Different Mechanical Mitral Valve Sounds on the Patients' Quality of Life
Authors: Huang Jiang-Shan , Hong Zhi-Nuan , Sun Kai-Peng , Cao Hua , Chen Qiang 
Published: September 2019

Background The purpose of this study was to compare the opening and closing sounds of three different mechanical mitral valves and to analyze their effects on the quality of life of patients.
Methods Ninety patients who underwent mechanical mitral valve replacement (MVR) were divided into three groups: those who received Advancing the Standard (ATS), St. Jude Medical (SJM), or Carbomedics (CM) mechanical valves. The sound pressure of the different valves was measured with a microphone. A detailed, self-designed questionnaire with a 1 to 10 analog scale and the 36-item Short Form Health Survey (SF-36) score was used to assess the quality of life of the patients.
Result The sound pressure measured by ATS was 64.4 ± 3.1 dB, while that measured by SJM, and CM was 64.5 ± 4.7 and 67.2 ± 5.3 dB, respectively. The overall subjective interference score in the ATS group was the lowest compared with that in the SJM and CM groups (ATS: 1.3 ± 0.9 dB, SJM: 3.9 ± 1.6 dB, CM: 4.1 ± 1.5 dB). Relatives and nonfamily members of the ATS group also had the lowest analog scale scores. Specific questions showed that the ATS valve had the least impact on sleep and daily life (impact on sleep: ATS: 4/30, SJM: 9/30, CM: 11/30, impact on daily life: ATS: 2/30, SJM: 8/30, CM: 8/30). The SF-36 score showed that there were significant differences in social functioning, role-emotional, mental health, and vitality.
Conclusion Patients receiving ATS mechanical MVR may be less disturbed by valve noise than those receiving SJM or CM mechanical MVR in a single Chinese cardiac center.
The negotiation of meaning in dialogue interpreting
Authors: Delizée Anne , Michaux Christine 
Published: September 2019

AbstractThe objective of this study is to contribute to a better understanding of the negotiation of meaning between the participants of a bilingual interpreter-mediated interaction by analysing the effects of the verbalization of the inferences by the interpreter. The conceptual tools of Relevance Theory were applied to three interpreted excerpts of Russian-French psychotherapeutic interactions. The results suggest that, by verbalizing inferences, the interpreter co-creates a shared cognitive environment, reinforces intra- and inter-discursive coherence, diminishes the cognitive efforts of the recipient, and encourages primary parties to cooperate. The analysis of the cognitive processes at work in the excerpts tends to show that what has so far been treated as the interpreter’s “additions” or “expanded renditions” enables the latter to exercise cooperative coordination of interaction, and could therefore be more precisely calledcollaborative renditions.
“A lot to think about”
Authors: Herring Rachel E. 
Published: September 2019

AbstractThis paper takes a process-focused approach to investigating the dialogue interpreting task from the perspective of the task performer. It reports on a portion of a research study aimed at better understanding the cognitive and interactional processes involved in dialogue interpreting. Eight interpreters interpreted a simulated interaction and subsequently completed a retrospective process-tracing session. The results shed light on the complexities of dialogue interpreting performance as experienced by the task performer, specifically with regard to interpreters’ online monitoring of affect, behavior, cognition, and context.
Response of deficit drip irrigation on production and growth parameters of capsicum (capsicum annuum) inside naturally ventilated polyhouse
Authors: Dimple  , Bhakar S R , Lakhawat S S , Rajput Jitendra , Mittal H K , Kothari Mahesh 
Published: September 2019

Abstract
A field experiment was conducted during March-August 2017 at Plasticulture farm College of Technology & Engineering, Udaipur. Randomized block design (RBD) was adopted for conducting field trial with six levels of irrigation viz., 100, 90, 80, 70, 60 and 100% (control without mulch) of crop evapotranspiration and 4 replication. Capsicum (shimla mirch locally known in Udaipur) cv Indra was irrigated with drip irrigation (also known as trickle irrigation) technique. Measurements of crop biometric parameters namely plant height, number of leaves and individual fruit weight was done at pre scheduled time throughout the crop season. Results revealed that biometric parameters found maximum in case of 80% irrigation level whiles minimum in 60% irrigation level treatment. The highest yields (42.64 t/ha) was also recorded in the same treatment. Therefore, it can conclude that by scheduling irrigation to meet 80% of crop ET will result in optimum crop yields and enhances crop water use efficiency of capsicum grown inside naturally ventilation polyhouse (NVPH).
Effect of ground heat exchanger for root-zone cooling on the growth and yield of aeroponically-grown strawberry plant under tropical greenhouse condition
Authors: Pascual C S , Agulto I C , Espino A N , Malamug V U 
Published: September 2019

Abstract
With the increasing cost of fossil fuel to generate power for cooling purposes, there is a need for alternative methods of cooling system that is sustainable and economically viable in producing high quality crops under tropical condition. This study aimed to investigate the effect of ground heat exchanger (GHE) for root-zone cooling on the growth, yield, and economic returns of aeroponically-grown strawberry plants under tropical greenhouse condition. The experiment had two treatments; the root-zone cooling methods (GHE, Air-conditioning Unit (ACU), and Control – no root-zone cooling) and photoperiods (8-hours, 16-hours, and 12-hours). We observed that the root-zone mean temperature under GHE was maintained at 26.75 °C, which was 2.61°C higher than ACU and 2.17°C lower than the control. The GHE and ACU did not significantly differ in terms of plant’s growth with larger and number of leaves, and longer petioles than the control. We found no significant interaction of the treatments on the yield nor difference between GHE and ACU with 181-186 g/plant. Projected benefit cost ratio using GHE is 4.24 and annual return of investment is at 32%. This study underscores the feasibility of GHE application for root-zone cooling under tropical greenhouse condition.