bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Contrast and Resolution Labeling of Amyloid Plaques in Tissue Sections from APP-PS1 Mice and Humans with Alzheimer’s Disease with the Zinc Chelator HQ-O: Practical and Theoretical Considerations
Authors: Schmued Larry , Raymick James , Sarkar Sumit 
Published: September 2019

Background:Various methodologies have been employed for the localization of amyloid plaques in numerous studies on Alzheimer’s disease. The majority of these stains are thought to label the plaques by virtue of their affinity for aggregated Aβ. However, plaques are known to contain numerous other components, including multivalent metals such as zinc.Objective:This investigates whether it is possible to localize the presence of zinc in parenchymal and vascular amyloid plaques in afflicted brains. To accomplish this, a novel fluorescent zinc chelator, HQO, was investigated to determine its mechanism of binding and to optimize a stain for the high contrast and resolution histological localization of amyloid plaques.Method:A novel zinc chelator, HQ-O, was developed for localizing zinc within amyloid plaques. The histology involves incubating tissue sections in a dilute aqueous solution of HQ-O. Its compatibility with a variety of other fluorescent methodologies is described.Results:All amyloid plaques are stained in fine detail and appear bright green under blue light excitation. The staining of parenchymal plaques correlates closely with that seen following staining with antibodies to Aβ, however, the HQ-O sometimes also label additional globular structures within blood vessels. In situ mechanistic studies revealed that fluorescent plaque-like structures are only observed with HQ-O when synthetic Aβx-42 is aggregated in the presence of zinc.Conclusion:Zinc is intimately bound to all amyloid plaques, which was demonstrated by its histological localization using a novel fluorescent zinc chelator, HQ-O. Additionally, the tracer is also capable of labeling intravascular leucocytes due to their high zinc content.
A Comparison of Ultrasound and Fluoroscopy-guided Celiac Plexus Neurolysis in Patients with Pancreatic Cancer
Authors: Elhossieny Khadeja M. , Seleem Waseem M. , Abd-Elsalam Sherief , Haydara Tamer , Gharbawy Nashwa Mohamed El 
Published: September 2019

Background & Aims:
Celiac plexus neurolysis is an elegant way of reducing pain in
patients with pancreatic cancer. The aim of this work was to compare the effectiveness of ultrasound
versus fluoroscopy-guided celiac plexus neurolysis in pancreatic cancer management.

Methods:
This study included 60 patients presenting with pancreatic cancer pain; who were subjected
to one session of celiac plexus neurolysis and were divided equally into two groups: -
Group (1): included 30 patients (12 femalesamp;18 males); who were exposed to ultrasound (US)-
guided celiac plexus neurolysis and group (2): included 30 patients (10 females & 20 males) who
were exposed to fluoroscopy-guided celiac plexus neurolysis. Abdominal pain was assisted by
visual analogue score (VAS).

Results:
Regarding VAS, our results revealed that all patients showed improvement after celiac
plexus neurolysis either through ultrasound technique or via percutaneous fluoroscopy technique.
Furthermore, the ultrasound group recorded more significant pain relief with improved VAS than
the fluoroscopy group immediately and on long-term follow-up with mean ± SD as follows: -
Immediately (9.2 ± 0.8) to (2.5 ± 0.7) vs. (9.1 ± 0.7) to (3.5 ± 0.82, respectively); After 1 week
(1.1 ± 0.8 vs. 3.6 ± 1.7, respectively), after 1 month ( 1 ± 0.9 vs. 3.7 ± 1.9), after three months (1.7
± 1.01 vs. 5.9 ± 1.7, respectively) and after 6 months (2.3 ± 0.6 vs. 7.5 ± 1.6, respectively).

Conclusion:
The study revealed that ultrasound-guided celiac plexus neurolysis is more durable,
tolerable, effective and safe compared to fluoroscopy-guided neurolysis of patient suffering from
pancreatic cancer pain.
Fluoroquinolone-3-carboxamide Amino Acid Conjugates: Synthesis, Antibacterial Properties And Molecular Modeling Studies
Authors: Bokhtia Riham M. , Panda Siva S. , Girgis Adel S. , Honkanadavar Hitesh H. , Ibrahim Tarek S. , George Riham F. , Kashef Mona T. , Fayad Walid , Sakhuja Rajeev , Abdel-Aal Eatedal H. , Al-Mahmoudy Amany M. M. 
Published: September 2019

Background:
Bacterial infections are considered as one of the major global health
threats, so it is very essential to design and develop new antibacterial agents to overcome the
drawbacks of existing antibacterial agents.

Method:
The aim of this work is to synthesize a series of new fluoroquinolone-3-carboxamide
amino acid conjugates by molecular hybridization. We utilized benzotriazole chemistry to synthesize
the desired hybrid conjugates.

Result:
All the conjugates were synthesized in good yields, characterized, evaluated for their antibacterial
activity. The compounds were screened for their antibacterial activity using methods
adapted from the Clinical and Laboratory Standards Institute. Synthesized conjugates were tested
for activity against medically relevant pathogens; Escherichia coli (ATCC 25922), Pseudomonas
aeruginosa (ATCC 27856) Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis
(ATCC 19433).

Conclusion:
The observed antibacterial experimental data indicates the selectivity of our synthesized
conjugates against E.Coli. The protecting group on amino acids decreases the antibacterial
activity. The synthesized conjugates are non-toxic to the normal cell lines. The experimental data
were supported by computational studies.
Extraction and Identification of Two Flavonoids in Phlomoides hyoscyamoides as an Endemic Plant of Iran: The Role of Quercetin in the Activation of the Glutathione Peroxidase, the Improvement of the Hydroxyproline and Protein Oxidation in Bile Duct-Ligated Rats
Authors: Doustimotlagh Amir H. , Taheri Salman , Mansourian Mahboubeh , Eftekhari Mahdieh 
Published: September 2019

Background:
Cholestatic liver disease, a serious chronic condition that develops progressive hepatic
degeneration through free radicals.

Objective:
The present study was designed to extract and identify two flavonoids in Phlomoides hyoscyamoides
plant, native to Iran and evaluate the role of quercetin identified on the liver injury among bile ductligated
rats.

Methods:
This study was conducted on 25 male Wistar rats within three groups of sham control, mere bile
duct-ligated, and bile duct-ligated with quercetin. The bile duct-ligated animals received quercetin at a dose
of 50 mg/kg/day for 10 days, followed by biochemical tests, oxidative stress markers, activity of antioxidant
enzymes and hematoxylin and eosin staining. Molecular docking was used to explore the interactive
behavior of quercetin with glutathione peroxidase.

Results:
According to analyses of the obtained extract, two main active ingredients of P. hyoscyamoides
were rutin and quercetin. Bile duct-ligated group showed a significant liver necrosis, a clear increase in
plasma and tissue oxidative stress parameters, and a decrease in glutathione peroxidase activity as compared
to sham control group. Quercetin injection in bile duct-ligated rats resulted in significant decrease in
hydroxyproline, protein carbonyl and histopathologic indexes and significant increase in glutathione peroxidase
activity (P-value≤0.05). Based on the molecular docking, the quercetin was able to regulate the glutathione
peroxidase activity.

Conclusion:
The quercetin acts as an enzyme inducer by renewing the glutathione peroxidase activity and
inhibiting the oxidation of proteins and hence decreases the oxidative stress. These results could be a sign
of confirming the positive role of quercetin in attenuating the liver damage and degeneration.
Combined QSAR Model and Chemical Similarity Search for Novel HMG-CoA Reductase Inhibitors for Coronary Heart Disease
Authors: Rajathei David Mary , Parthasarathy Subbiah , Selvaraj Samuel 
Published: September 2019

Background:Coronary heart disease generally occurs due to cholesterol accumulation in the walls of the heart arteries. Statins are the most widely used drugs which work by inhibiting the active site of 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGCR) enzyme that is responsible for cholesterol synthesis. A series of atorvastatin analogs with HMGCR inhibition activity have been synthesized experimentally which would be expensive and time-consuming.Methods:In the present study, we employed both the QSAR model and chemical similarity search for identifying novel HMGCR inhibitors for heart-related diseases. To implement this, a 2D QSAR model was developed by correlating the structural properties to their biological activity of a series of atorvastatin analogs reported as HMGCR inhibitors. Then, the chemical similarity search of atorvastatin analogs was performed by using PubChem database search.Results and Discussion:The three-descriptor model of charge (GATS1p), connectivity (SCH-7) and distance (VE1_D) of the molecules is obtained for HMGCR inhibition with the statistical values of R2= 0.67, RMSEtr= 0.33, R2 ext= 0.64 and CCCext= 0.76. The 109 novel compounds were obtained by chemical similarity search and the inhibition activities of the compounds were predicted using QSAR model, which were close in the range of experimentally observed threshold.Conclusion:The present study suggests that the QSAR model and chemical similarity search could be used in combination for identification of novel compounds with activity by in silico with less computation and effort.
Current State of the Art for Survival Prediction in Cancer Using Data Mining Techniques
Authors: Doja M.N. , Kaur Ishleen , Ahmad Tanvir 
Published: September 2019

Background:
Cancer treatment is expensive and results in a lot of side effects, and thus
survival prediction is necessary for the patients as well as the clinician. Data mining technology
has been used in the medical domain to extract interesting information. Cancer prognosis is such
an application in medicine.

Objective:
This study focuses on identifying the technologies used in the recent past for predicting
the survival of cancer patients. Supervised, semi-supervised and unsupervised techniques have
been used over the years successfully for the survival prediction of different types of cancer.

Methods:
A systematic literature review process has been followed in this study to discover the
future directions of the research. This study focuses on uncovering the gaps in recent studies.

Results and Conclusion:
It has been found that the present system lacks structured information of
the patients. Also, there are a lot of different cancer types that are still unexplored in terms of
survival prediction, mainly due to the unavailability of sufficient data. Hence a lot can be
improved if researchers may get their hands on required data for the research.
Modifications in the Structural and Optical Properties of ZnO Nanophosphor on Doping with Tb
Authors: Rani Suman , Lal Bansi , Saxena Sumit , Shukla Shobha 
Published: September 2019

Background:The characteristic visible emission from ZnO being attributed to the defect energy states can be tailored by doping as well as by synthesis techniques. Rare-earth elements, among various dopants, are interesting because of their unique emission properties in the visible region. Terbium (Tb), in particular, is reported to contribute significantly to the creation of the defect energy states when doped in ZnO. This study investigated the Tb concentration dependent modifications in the structural and optical properties of ZnO nanophosphor.Methods:Tb (0.1, 0.5, 01.0 mol%) doped nanophosphor powder samples prepared by low temperature precipitation method, were sintered in air at 700oC using a home-built temperature controlled (±1oC) muffle furnace. Powder XRD and EDX spectra at room temperature were recorded using Philips X perts x-ray spectrometer while Jeol JSM-7600F was used to record SEM images. Photoluminescence spectra excited by the 280, 300, 380 and 460nm radiation from a Xe lamp were recorded using Carry 8000 spectrophotometer. Raman spectra excited by 514.5nm radiation from an Ar-ion laser, was investigated using Morrison microscope Olympus Bx 41 while UV-VIS absorption spectra were recorded on UV- 1800 UV-VIS Spectrophotometer.Results:FTIR and XRD spectra showed that the basic ZnO wurtzite crystal structure remained unchanged on doping. However, XRD data analysis indicated that the 0.1 mol% Tb might be incorporated in ZnO unit cell at an interstitial and / or substitutional site(s) while at 0.5 and 1.0 mol% doping levels migration of Tb to the surface could be the dominant process. This was further confirmed by Raman and photoluminescence studies. Broad emission (122nm FWHM) peaking around 510nm was observed when the doped samples were excited with 280 and 300nm radiation while characteristic ZnO emission was observed with 380 and 460nm radiation. The calculated chromaticity color coordinates (x,y) of the emission excited by 280nm in 0.5 mol% doped ZnO were: x=0.29 and y=0.31, which are very close to those of the daylight at noon.Conclusion:Concentration dependent lattice distortions were observed; it was concluded that at 0.1mol% concentration level Tb was incorporated in ZnO lattice resulting in interstitial or substitutional defects. On the other hand, at 0.5 and 1.0 mol% doping levels diffusion of Tb to the surface producing strain due to "hydrostatic like pressure" seemed to be the dominating process; maximum strain was observed at 0.5mol% doping. The calculated chromaticity color coordinates of the 280nm excited emission from ZnO:Tb (0.5mol%) were found to be very close to those of the "day light at noon” indicating the suitability of the material for the realization of white light sources.
Silica gel Supported Boric tri-Sulfuric Anhydride (SiO2-BTSA): An Efficient Catalytic System for the Synthesis of Phloroglucide Analogs as Antimicrobial Agents
Authors: Bargebid Rahele , Khalafi-Nezhad Ali , Zomorodian Kamiar , Moradi Mahkameh , Khabnadideh Soghra 
Published: September 2019

Background:
Polyhydroxy aromatic compounds are one of the most important classes
of phenolic compounds with different biological activities. Some important and biologically active
phenol-based compounds have also been isolated from nature.

Methods:
An efficient procedure for the synthesis of polyhydroxy aromatic compounds (phloroglucide
analogs) is described. In this procedure, a reaction took place between different 4-substituted
phenols and 2,6-bis(hydroxymethyl) phenols. The reactions proceed in the presence of catalytic
amount of silica gel supported boric tri-sulfuric anhydride (SiO2-BTSA) in excellent yields.

Results:
16 compounds were synthesized (I1-I16). Chemical structures of all the compounds were
confirmed by spectroscopic methods. We optimized the chemical reactions in the presence of different
acidic catalysts, different solvents and also different temperatures. A catalytic amount of
SiO2-BTSA in dichloroethane (DCE) was the best condition. Some of the synthesized compounds
were screened for their antimicrobial activities. Antifungal and antibacterial activities of the synthesized
compounds were evaluated by broth microdilution method as recommended by CLSI.
Some of the tested compounds showed good in vitro biological properties.

Conclusion:
Our active compounds could introduce as good candidates for further studies as antimicrobial
agents.
Localization and Tracking of Mobile Jammer Sensor Node Detection in Multi-Hop Wireless Sensor Network
Authors: Porkodi K.P. , Karthika I. , Gianey Hemant K. 
Published: September 2019

Background:
The jammer in a wireless sensor network is located and tracked with open access
and shared nature of the wireless medium. The existing algorithms mainly track the stationary
jammer. Mobile jammer often moves from one place to another becoming difficult. Mobile jammer location
tracker algorithm is proposed to find the location of a mobile jammer with four steps selection
i.e., initial examining node, determination of supporting node, trilateration localization and examining
group handover.

Objective:
In this research paper an algorithm is proposed for finding the location of mobile jammer in
wireless sensor network. Finding location faces a huge difficulty due to non-supportive working between
the multi hop wireless sensor network and jammer. The existing algorithms are used to find the location of
stationary jammers only. Mobile jammers frequently change their position from time to time. Therefore
jamming increases between the node to node communications in the multi hop wireless sensor network.

Methods:
The multi hop wireless sensor network is deployed with n number of stationary nodes in a particular
area. The omni directional antenna is fitted with those stationary nodes and the transmission
powers of the nodes are also constant. The direction-finding table is maintained and keep on updating
for an interval of each node. The position of the nodes is tracked with the GPS devices or by existing location
finding algorithm. The nodes in the multi hop wireless sensor network are installed in A*A square
area uniformly and randomly. The transmitting node is tp node. The received signal to noise ratio is
threshold STNR. Neighbor list records the neighbor node of the multi hop wireless sensor networks.

Results and Conclusion:
The proposed idea of localization and tracking system of mobile jammer is estimated
efficiently by the multi hop wireless sensor network with the simulator MATLAB. The nodes in
multi hop wireless sensor network are installed in A*A area square uniformly and randomly.
Evaluation of a Health Information Technology–Enabled Panel Management Platform to Improve Anticoagulation Control in a Low-Income Patient Population: Protocol for a Quasi-Experimental Design
Authors: Fontil Valy , Kazi Dhruv , Cherian Roy , Lee Shin-Yu , Sarkar Urmimala 
Published: September 2019

Background
Warfarin is one of the most commonly prescribed medications in the United States, and it causes a significant proportion of adverse drug events. Patients taking warfarin fall outside of the recommended therapeutic range 30% of the time, largely because of inadequate laboratory monitoring and dose adjustment. This leads to an increased risk of blood clots or bleeding events. We propose a comparative effectiveness study to examine whether a technology-enabled anticoagulation management program can improve long-term clinical outcomes compared with usual care.

Objective
Our proposed intervention is the implementation of an electronic dashboard (integrated into a preexisting electronic health record) and standardized workflow to track patients’ laboratory results, identify patients requiring follow-up, and facilitate the use of a validated nomogram for dose adjustment. The primary outcome of this study is the time in therapeutic range (TTR) at 6 months post intervention (a validated metric of anticoagulation quality among patients receiving warfarin).

Methods
We will employ a pre-post quasi-experimental design with a nonequivalent usual-care comparison site and a difference-in-differences approach to compare the effectiveness of a technology-enabled anticoagulation management program compared with usual care at a large university-affiliated safety-net clinic.

Results
We used a commercially available health information technology (HIT) platform to host a registry of patients on warfarin therapy and create the electronic dashboard for panel management. We developed the intervention with, and for, frontline clinician users, using principles of human-centered design. This study is funded until September 2020 and is approved by the University of California, San Francisco Institutional Review Board until June 22, 2020. We completed data collection in September 2019 and expect to complete our proposed analyses by February 2020.

Conclusions
We anticipate that the intervention will increase TTR among patients taking warfarin and that the use of this HIT platform will facilitate tracking and monitoring of patients on warfarin, which could enable outreach to those overdue for visits or laboratory monitoring. We will use these findings to iteratively improve the platform in preparation for a larger, multiple-site, pragmatic clinical trial. If successful, our study will demonstrate the integration of HIT platforms into existing electronic health records to improve patient care in real-world clinical settings.

International Registered Report Identifier (IRRID)
DERR1-10.2196/13835
Exploring How Professionals Within Agile Health Care Informatics Perceive Visualizations of Log File Analyses: Observational Study Followed by a Focus Group Interview
Authors: ten Klooster Iris , Noordzij Matthijs Leendert , Kelders Saskia Marion 
Published: September 2019

Background
An increasing number of software companies work according to the agile software development method, which is difficult to integrate with user-centered design (UCD) practices. Log file analysis may provide opportunities for integrating UCD practices in the agile process. However, research within health care information technology mostly has a theoretical approach and is often focused on the researcher’s interpretation of log file analyses.

Objective
We aimed to propose a systematic approach to log file analysis in this study and present this to developers to explore how they react and interpret this approach in the context of a real-world health care information system, in an attempt to answer the following question: How may log file analyses contribute to increasing the match between the health care system and its users, within the agile development method, according to agile team members?

Methods
This study comprised 2 phases to answer the research question. In the first phase, log files were collected from a health care information system and subsequently analyzed (summarizing sequential patterns, heat mapping, and clustering). In the second phase, the results of these analyses are presented to agile professionals during a focus group interview. The interpretations of the agile professionals are analyzed by open axial coding.

Results
Log file data of 17,924 user sessions and, in total, 176,678 activities were collected. We found that the Patient Timeline is mainly visited, with 23,707 (23,707/176,678; 13.42%) visits in total. The main unique user session occurred in 5.99% (1074/17,924) of all user sessions, and this comprised Insert Measurement Values for Patient and Patient Timeline, followed by the page Patient Settings and, finally, Patient Treatment Plan. In the heat map, we found that users often navigated to the pages Insert Measurement Values and Load Messages Collaborate. Finally, in the cluster analysis, we found 5 clusters, namely, the Information-seeking cluster, the Collaborative cluster, the Mixed cluster, the Administrative cluster, and the Patient-oriented cluster. We found that the interpretations of these results by agile professionals are related to stating hypotheses (n=34), comparing paths (n=31), benchmarking (n=22), and prioritizing (n=17).

Conclusions
We found that analyzing log files provides agile professionals valuable insights into users’ behavior. Therefore, we argue that log file analyses should be used within agile development to inform professionals about users’ behavior. In this way, further UCD research can be informed by these results, making the methods less labor intensive. Moreover, we argue that these translations to an approach for further UCD research will be carried out by UCD specialists, as they are able to infer which goals the user had when going through these paths when looking at the log data.