bluuebunny/crossref_metadata_2025_split huggingface dataset:
Autogenous Bone Cranioplasty: Review of a 42-Year Experience by a Single Surgeon
Authors: Cabbad Nicole C. , Stalder Mark W. , Arroyave Aaron , Wolfe Erin M. , Wolfe S. Anthony 
Published: July 2019

Background:
Autogenous bone is frequently espoused as the gold standard material for cranioplasty procedures, yet alloplastic cranioplasty continues to persist in the search, presumably, for a simpler technique. Although short-term outcomes can be successful using foreign materials, long-term follow-up in these patients often demonstrates increased rates of failure because of exposure or late infection. Autogenous bone grafts, however, integrate and revascularize, and are thus more resistant to infection than alloplastic materials.

Methods:
This is a retrospective review of all patients that underwent reconstructive cranioplasty for full-thickness defects, as performed by the senior author (S.A.W.) between 1975 and 2018. All procedures were performed with autogenous bone.

Results:
One hundred fifty-four patients met criteria for inclusion in the report. Cranioplasties were performed for both congenital and secondary indications. Split calvaria was used in 115 patients (74.7 percent), rib graft was used in 12 patients (7.8 percent), iliac crest graft was used in 10 patients (6.5 percent), and combinations of donor-site grafts were used in 17 patients (11.0 percent). In the entire series, none of the patients suffered from complications related to infection of either the donor site or transferred bone graft. None of the patients required secondary operations to fill in defects created by the postoperative resorption.

Conclusions:
Although autologous bone is widely considered the gold standard material for cranioplasty procedures, some argue against its use, mainly citing unpredictable resorption as the purported disadvantage. However, it is less susceptible to infection, and results in fewer long-term complications than alloplastic materials. There is no alloplastic material that has matched these outcomes, and thus autogenous bone should be considered as the primary option for cranioplasty procedures.

CLINICAL QUESTION/LEVEL OF EVIDENCE:
Therapeutic, IV.
Oncofetal Protein CR-1 in a new clinical role: a potential tumor marker for Oral Squamous Cell Carcinoma
Authors: Anu Jain , Kumar Mallupattu Sumanth , Reetu Thakur , Satyawati Mohindra , Amanjit Bal , Ashim Das , Sushmita Ghoshal , Arnab Pal 
Published: July 2019

AbstractPURPOSECR-1 (CR-1) is an oncofetal protein with its role as a key factor in early process of carcinoma has been evaluated in cases of various cancers. However, very few studies have reported its role in oral cancers, which is the sixth most common cancer around the world, particularly with high prevalence in developing countries. Oral squamous cell carcinoma (OSCC) is the most predominant (90%) of all the histological types of oral cancer. Late detection, associated with increased morbidity and mortality, is mainly attributed to non-availability of a suitable biomarker for the disease. In the present pilot study we have evaluated the role of soluble CR-1, in serum as a potential tumor marker for OSCC.METHODSCR-1 was estimated using sandwich ELISA in serum samples of 50 biopsy proven OSCC patients (pre and post treatment) along with age and gender matched healthy controls. Immunohistochemistry was also done in corresponding tumor tissue sections to check the expression of CR-1.RESULTSPre-treatment CR-1 was found to be 2.25 fold higher in serum of OSCC patients as compared to control (p<0.0001***), which was reduced to 1.6 folds post treatment (p=0.0006***). CR-1 levels were comparatively higher in early stage of disease. Upon IHC 80% of the cases were found to be positive for CR-1.CONCLUSIONThis study provides evidence that serum levels of CR-1 are elevated in patients of Oral Squamous Cell Carcinoma, which decrease post treatment. Also, the association of expression of protein with tumor progression predicts CR-1 as a molecule that can be further evaluated as a potential tumor maker in OSCC.
B cell humoral response and differentiation is regulated by the non-canonical poly(A) polymerase TENT5C
Authors: Bilska Aleksandra , Kusio-Kobiałka Monika , Krawczyk Paweł S. , Gewartowska Olga , Tarkowski Bartosz , Kobyłecki Kamil , Gruchota Jakub , Borsuk Ewa , Dziembowski Andrzej , Mroczek Seweryn 
Published: July 2019

SummaryTENT5C is a non-canonical cytoplasmic poly(A) polymerase (ncPAP) upregulated in activated B cells and suppressing their proliferation. Herein we measured the global distribution of poly(A) tail lengths in responsive B cells using a modified Nanopore direct RNA-sequencing approach and revealed that TENT5C polyadenylates immunoglobulin mRNAs regulating their steady-state levels. Consequently, TENT5C deficient B cells secrete less antibodies and KO mice have diminished gamma globulin concentrations despite the increased number of CD138high plasma cells as a consequence of accelerated differentiation. TENT5C is explicitly upregulated in differentiating plasma cells by innate signaling. Importantly, TENT5C deficiency in B lymphocytes impairs the capacity of the secretory pathway through the reduction of ER volume and downregulation of unfolded protein response.Our findings define the role of the TENT5C enzyme in B cell physiology and discover the first ncPAP engaged in the regulation of immunoglobulin mRNA poly(A) tails, thus serving as a regulator of humoral immunity.
Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
Authors: Machado Leopoldo F. M. , Currin Andrew , Dixon Neil 
Published: July 2019

AbstractBackgroundGenetically encoded biosensors are useful tools for the detection of metabolites and industrially valuable molecules, and present many potential applications in biotechnology and biomedicine. However, the most common approach to develop biosensors relies on employing a limited set of naturally occurring allosteric transcript factors (aTFs). Therefore, altering the substrate specificity of aTFs towards the detection of new effectors is an important goal.ResultsHere, the PcaV repressor, a member of the MarR aTF family, was used to develop a biosensor for the detection of hydroxyl-substituted benzoic acids, including protocatechuic acid (PCA). The PCA biosensor was further subjected to directed evolution to alter its substrate specificity towards vanillin and other closely related aromatic aldehydes, to generate the Van2 biosensor. Substrate recognition of Van2 was explored in vitro using a range of biochemical and biophysical analyses, and extensive in vivo genetic-phenotypic analysis was performed to determine the role of each amino acid change upon biosensor performance.ConclusionsThis is the first study to report directed evolution of a member of the MarR aTF family, and demonstrates the plasticity of the PCA biosensor by altering its substrate specificity to generate a biosensor for aromatic aldehydes.
One week of chronic sleep debt does not affect decision-making processes in a mouse version of the Iowa Gambling Task
Authors: Pittaras Elsa , Granon Sylvie , Rabat Arnaud 
Published: July 2019

SummarySocio-professional pressures push people to sleep less which leads to chronic sleep debt (CSD) for a significant percentage of the population. Although the health consequences of CSD are well known, research shows that high-level cognitive processes in humans are more affected by acute sleep debt (ASD) rather than CSD (Drake et al., 2001). We have previously shown that ASD has deleterious effects on decision-making in mice and that some mice were more sensitive to ASD than others (Pittaras et al., 2018) by using a rodent version of the Iowa Gambling Task (Bechara et al., 1994). In this study, we showed that, as in humans, CSD has fewer effects on decision-making compared to ASD. We hypothesize that this observation was due to the set-up of a compensatory mechanism.
Accelerated viral dynamics in bat cell lines, with implications for zoonotic emergence
Authors: Brook Cara E. , Boots Mike , Chandran Kartik , Dobson Andrew P. , Drosten Christian , Graham Andrea L. , Grenfell Bryan T. , Müller Marcel A. , Ng Melinda , Wang Lin-Fa , van Leeuwen Anieke 
Published: July 2019

AbstractBats host virulent zoonotic viruses without experiencing disease. A mechanistic understanding of the impact of bats’ virus hosting capacities, including uniquely constitutive immune pathways, on cellular-scale viral dynamics is needed to elucidate zoonotic emergence. We carried out virus infectivity assays on bat cell lines expressing induced and constitutive immune phenotypes, then developed a theoretical model of ourin vitrosystem, which we fit to empirical data. Best fit models recapitulated expected immune phenotypes for representative cell lines, supporting robust antiviral defenses in bat cells that correlated with higher estimates for within-host viral propagation rates. In general, heightened immune responses limit pathogen-induced cellular morbidity, which can facilitate the establishment of rapidly-propagating persistent infections within-host. Rapidly-replicating viruses that have evolved with bat immune systems will likely cause enhanced virulence following emergence into secondary hosts with immune systems that diverge from those unique to bats.
Structure of nucleotide-bound Tel1ATM reveals the molecular basis of inhibition and structural rationale for disease mutations
Authors: Yates Luke A. , Williams Rhys M. , Hailemariam Sarem , Ayala Rafael , Burgers Peter , Zhang Xiaodong 
Published: July 2019

SUMMARYYeast Tel1 and its highly conserved human orthologue ATM are large protein kinases central to the maintenance of genome integrity. Mutations in ATM are found in ataxia-telangiectasia (A-T) patients and ATM is one of the most frequently mutated genes in many cancers. Using cryo electron microscopy, we present the structure of Tel1 in a nucleotide-bound state. Our structure reveals molecular details of key residues surrounding the nucleotide binding site and provides a structural and molecular basis for its intrinsically low basal activity. We show that the catalytic residues are in a productive conformation for catalysis, but the PIKK-regulatory domain-Insert (PRD-I) restricts peptide-substrate access and the N-lobe is in an open conformation, thus explaining the requirement for Tel1 activation. Structural comparisons with other PIKKs suggest a conserved and common allosteric activation mechanism. Our work also provides a structural rationale for many mutations found in A-T and cancer.
convertibleCARs: A chimeric antigen receptor system for flexible control of activity and antigen targeting
Authors: Landgraf Kyle E. , Williams Steven R. , Steiger Daniel , Gebhart Dana , Lok Stephen , Martin David W. , Roybal Kole T. , Kim Kaman Chan 
Published: July 2019

ABSTRACTWe have developed a chimeric antigen receptor (CAR) platform that functions as a modular system to address limitations of current CAR therapies. An inert form of the NKG2D extracellular domain (iNKG2D) was used as the ectodomain of the CAR to generate convertibleCAR™-T cells. These cells were activated only when an immunological synapse was formed with an antigenic target, mediated by a bispecific adaptor comprised of an iNKG2D-exclusive ULBP2-based ligand fused to an antigen-targeting antibody (MicAbodyTM). Efficacy against Raji tumors in NSG mice was dependent upon doses of both a rituximab-based MicAbody and convertibleCAR-T cells. We have also demonstrated that the exclusive ligand-receptor partnering enabled the targeted delivery of a mutant form of IL-2 to exclusively promote the expansion of convertibleCAR-T cells in vitro and in vivo. By altering the Fv domains of the MicAbody or the payload fused to the orthogonal ligand, convertibleCAR-T cells can be readily targeted or regulated.
Phase discontinuities underlie increased drowsiness and diminished sleep quality in older humans
Authors: Sanders Teresa Hinkle 
Published: July 2019

AbstractHealthy humans switch seamlessly between activity states, wake up and fall asleep with regularity, and cycle through sleep stages necessary for restored homeostasis and memory consolidation each night. This study tested the hypothesis that such smooth behavioral transitions are accompanied by smooth transitions between stable neural states within the brain. A method for detecting phase discontinuities across a broad range of frequencies was created to quantify phase disruptions in the Fp-Cz EEG channel from 20 annotated sleep files. Phase discontinuities decreased with increasingly deep sleep, and increased phase discontinuity was associated with increased drowsiness, reduced deep sleep, and shorter REM sleep. A 10s phase discontinuity summary measure (the phase jump indicator) closely tracked the annotated sleep stages and enabled discrimination between short (< 10 min) and longer REM periods. Overall phase discontinuity correlated inversely with broadband EEG power, suggesting that reduced spurious signaling may facilitate increased synchronization. However, the correlation between phase discontinuity and power varied with sleep stage and age. Older individuals spent significantly more time in the Awake and Drowsy stages and less time in the deepest sleep stage and REM sleep. Interestingly, although EEG power was reduced in older individuals across all sleep stages, increased phase discontinuity only occurred in stages that showed impairment. In older patients the power vs. phase discontinuity correlation shifted to positive during drowsiness, suggesting potential deficits in cortical inhibition. These results provide evidence that phase discontinuity measures extend current capabilities for assessing sleep and may yield new insights into pathological brain states.Significance statementEvidence continues to accumulate regarding the positive relationship between healthy sleep and brain function. Recent studies also show that more healthful sleep can be induced with timely application of non-invasive therapies. Accordingly, the ability to accurately assess sleep quality in real-time has become increasingly important. Here, a newly defined measure, referred to as phase discontinuity, enabled rapid identification of unhealthful neural patterns associated with increased drowsiness, reduced deep sleep, and early termination of REM sleep. Moreover, the measure was linked to underlying neuronal and circuit properties known to impact sleep quality. Thus, the phase discontinuity measure defined in this study provides new insight into sleep pathology and has potential implications for closed-loop therapeutic intervention.
Overexpressing CsGH3.1 and CsGH3.1L reduces susceptibility to Xanthomonas citri subsp. citri by repressing auxin signaling in citrus (Citrus sinensis Osbeck)
Authors: Zou Xiuping , Long Junhong , Zhao Ke , Peng Aihong , Chen Min , Long Qin , He Yongrui , Chen Shanchun 
Published: July 2019

AbstractThe auxin early response gene Gretchen Hagen3 (GH3) plays dual roles in plant development and responses to biotic or abiotic stress. It functions in regulating hormone homeostasis through the conjugation of free auxin to amino acids. In citrus, GH3.1 and GH3.1L play important roles in responding to Xanthomonas citri subsp. citri (Xcc). Here, in Wanjingcheng orange (C. sinensis Osbeck), the overexpression of CsGH3.1 and CsGH3.1L caused increased branching and drooping dwarfism, as well as smaller, thinner and upward curling leaves compared with wild-type. Hormone determinations showed that overexpressing CsGH3.1 and CsGH3.1L decreased the free auxin contents and accelerated the Xcc-induced decline of free auxin levels in transgenic plants. A resistance analysis showed that transgenic plants had reduced susceptibility to citrus canker, and a transcriptomic analysis revealed that hormone signal transduction-related pathways were significantly affected by the overexpression of CsGH3.1 and CsGH3.1L. A MapMan analysis further showed that overexpressing either of these two genes significantly downregulated the expression levels of the annotated auxin/indole-3-acetic acid family genes and significantly upregulated biotic stress-related functions and pathways. Salicylic acid, jasmonic acid, abscisic acid, ethylene and zeatin levels in transgenic plants displayed obvious changes compared with wild-type. In particular, the salicylic acid and ethylene levels involved in plant resistance responses markedly increased in transgenic plants. Thus, the overexpression of CsGH3.1 and CsGH3.1L reduces plant susceptibility to citrus canker by repressing auxin signaling and enhancing defense responses. Our study demonstrates auxin homeostasis’ potential in engineering disease resistance in citrus.
Aero-heating modelling on the ablative noses during flight trajectory
Authors: Doustdar Mohamad Mehdi , Mardani Morteza , Ghadak Farhad 
Published: July 2019

Purpose
The purpose of this paper is to present the more accurate estimation of aero-heating for the ablative 3-D noses by using the viscous shock layers and similarity of viscous boundary layer methods.

Design/methodology/approach
The combination of viscous shock layer, similarity of viscous boundary layer (SVBL) methods, Park ablation and Baldwin–Lomax turbulent models is presented in this paper. The proposed method reduces computational memory and run time as compared to the time marching algorithms during flight trajectory. Therefore, the space marching algorithm and finite difference method is used, and the governing equations are transferred into curvature coordinate by using the mapping terms.

Findings
The solving for an ogive nose during flight trajectory shows that the convergence of this technique is fast as compared to the user defined function based on the fluent solvers, program to axisymmetric regular geometry code and other research. The results of this research are validated by the mentioned research studies. The relative error for the aero-heating, species concentration of the shock layer gas mixture because of dissociation/ionization of air and surface ablation results is less than 6, 5 and 11 per cent, respectively.

Research limitations/implications
The required time for an aerodynamic design of hypersonic noses reduces as the induced aero-heating is one of the principal design parameters in standpoint aerodynamic, structural and other terms. The magnitude of this parameter, surface temperature and surface recess because of ablation should be corrected during flight trajectory.

Social implications
The results of this research are applicable for aerospace industries.

Originality/value
The originality of this paper is 90 per cent.
The impact of corporate governance mechanisms on real and accrual earnings management practices: evidence from Jordan
Authors: Al-Haddad Lara , Whittington Mark 
Published: July 2019

PurposeThis paper aims to investigate the impact of corporate governance (CG) mechanisms on real (REM), accrual-based earnings management (AEM) and REM/AEM interaction in Jordan following the 2009 Jordanian CG Code (JCGC).Design/methodology/approachThe study used a sample of 108 Jordanian public firms covering 2010-2014. Hypotheses are tested using pooled OLS-regression models.FindingsThe authors find that both institutional and managerial ownership constrain the use of REM and AEM. In contrast, both independent directors and large shareholders are found to exaggerate such practices, and CEO-duality is found to exaggerate REM only. However, foreign ownership does not appear to have a significant impact. They further find that managers use REM and AEM jointly to obtain the greatest earnings impact.Practical implicationsThe findings have important implications for policymakers, regulators, audit professionals and investors in their attempts to constrain earnings management (EM) practices and improve financial reporting quality in Jordan.Originality/valueThe authors believe this to be the first Jordanian study examining the relationship between CG mechanisms and both REM and AEM following the introduction of the 2009 JCGC, as well as the first in Jordan and the Middle East to examine board characteristics and REM. Moreover, it is the first to test for the potential substitution of REM and AEM since the 2009 JCGC enactment. As such, the findings draw attention to EM practices and the role of monitoring mechanisms in Jordan.
Co-creation experiences in the music business: a systematic literature review
Authors: Saragih Harriman 
Published: July 2019

PurposeVarious literature have contextualised value co-creation concept in the music business and how that notion puts into practice in benefitting the actors in this particular business. The purpose of this paper is to review the extant literature to comprehend regarding the applicability of co-creation in music business which can be used to map and evaluate strategies used to stimulate and exercise co-creation experiences; focus from such co-creational activities; stages during which co-creation occurred; channels in which the music as cultural product is delivered; and the co-creative platform used that can be useful for practitioners as well as scholars in the music business.Design/methodology/approachOf the available academic databases that exist on the online platform, this study takes into account six scholarly databases, i.e., Emerald, EBSCO, ISI Web of Sciences, ProQuest, ScienceDirect and Scopus.FindingsHaving filtered through the initial 113 papers that fulfil the predetermined criteria, this study discovers 33 empirical journal and proceeding papers that have discussed the co-creation concept in the music context from 2011 to 2017.Practical implicationsThe review practically implies that practitioners as well as scholars in the music or marketing field can first begin with planning and understanding the right strategy, focus, stage, channels and platforms before executing co-creational activities in the music business. This paper also speaks to the broader literature, particularly in the creative industries, that value co-creation can serve to be used to obtain monetary, experience or social value in the market using virtual and physical co-creative platforms. Other sectors in the creative industries can also infer that co-creation can be promoted and exercised through various orchestration strategies in several stages of the value chain.Originality/valueThis paper is the first to integrate five practical criteria as to how co-creation is applied and contextualised in the music business. It also contributes to the academic literature by presenting an exhaustive selective review of the value co-creation concept and its applicability to the music business.