bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profiling of serum antibodies against human papillomavirus antigens in Korean women with cervical intraepithelial neoplasia and cervical cancer
Authors: Jin Yingji , Choi Jae Woong , Kim Hyoung Jin , Eddouzi Jamel , Kim Seung Cheol , Ju Woong , Kim Yun Hwan , Kim Hong‐Jin 
Published: October 2018

AbstractSero‐epidemiological studies of human papillomavirus (HPV) have been undertaken over the last two decades. In this study, the prevalences of nine serum antibodies (anti‐E6, E7 and L1 antibodies of HPV types 16, 18, and 58) were evaluated in normal (control) Korean women and women with cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III, and cervical cancer. The frequencies of all types of anti‐HPV antibodies were higher in the CIN stages and cervical cancer than in normal women, and those of anti‐HPV16 E6 and E7, anti‐HPV18 E6 and E7, and anti‐HPV58 E7 antibodies were higher in the cervical cancer group than in the CIN stages. The frequencies of antibodies against HPV16, 18, and 58 E7 tended to increase with increasing severity of cervical lesions. However, there were few differences in the frequencies of antibodies against the L1 antigens of HPV16, 18 and 58 in cervical cancer versus CIN stages. The anti‐HPV antibodies were detected in 26.5% of normal, 46.3% of CIN I, 62.5% of CIN II, 51.6% of CIN III, and 75% of cancers when any of the nine antigens was used as a criterion. Correlations between HPV DNA positivity and seropositivity for anti‐HPV E6, E7, or L1 antibodies were found only in HPV16 DNA‐positive cervical cancers for anti‐HPV16 E6 and L1 antibodies. In addition, strong positive correlations in seropositivity were found between anti‐HPV16 E7 and anti‐HPV58 E7 antibodies, and between anti‐HPV18 E6 and anti‐HPV58 E6 antibodies. These findings should advance global profiling of the seroprevalences of antibodies against HPV antigens.
WAVE2 is associated with poor prognosis in pancreatic cancers and promotes cell motility and invasiveness via binding to ACTN4
Authors: Taniuchi Keisuke , Furihata Mutsuo , Naganuma Seiji , Saibara Toshiji 
Published: October 2018

AbstractWAVE2 is a member of the WASP/WAVE family of actin cytoskeletal regulatory proteins; unfortunately, little is known about its function in pancreatic cancers. In this study, we report the role of WAVE2 in the motility and invasiveness of pancreatic cancer cells. High WAVE2 expression in human pancreatic cancer tissues was correlated with overall survival. WAVE2 accumulated in the cell protrusions of pancreatic cancer cell lines. Downregulation of WAVE2 by small interfering RNA decreased the cell protrusions and inhibited the motility and invasiveness of pancreatic cancer cells. WAVE2 promoted pancreatic cancer cell motility and invasion by forming a complex with the actin cytoskeletal protein alpha‐actinin 4 (ACTN4). Downregulation of ACTN4 by small interfering RNA also inhibited the motility and invasiveness of the cells through a decrease in cell protrusions. Further investigation showed that WAVE2/ACTN4 signaling selectively stimulated p27 phosphorylation and thereby increased the motility and invasiveness of the cells. These results suggest that WAVE2 and ACTN4 stimulate p27 phosphorylation and provide evidence that WAVE2 promotes the motility and invasiveness of pancreatic cancer cells.
Effects of bran hydration and autoclaving on processing quality of Chinese steamed bread and noodles produced from whole grain wheat flour
Authors: Zhang Yan , Gao Fengmei , He Zhonghu 
Published: October 2018

Background and objectivesConsumption of Chinese steamed bread (CSB) and noodles made from whole grain wheat flour (WWF) is encouraged due to their important nutritional elements in the outer seed layers that benefit human health. However, the use of WWF is limited because of the poor processing quality and less attractive product properties of dark color, rough texture and coarse crumb structure. Three Chinese wheat cultivars, Zhongmai 895, Yumai 49, and Zhoumai 27, were used to investigate the potentials of bran hydration and autoclaving treatments for the improvement of WWF functionality in making CSB and noodles.FindingsBran particle size, hydration, and autoclaving treatment of WWF significantly affected the quality of dough, CSB, and noodles made from it. Farinograph water absorption and stability of WWFs with fine bran were larger than those with coarse bran. WWFs with coarse bran compared with fine bran exhibited inferior color and higher water retention capacity (WRC), and produced CSB with better texture and crumb structure and noodles with less smoothness and poor appearance. Prehydration of bran decreased dough water absorption and increased dough stability. WWFs containing bran hydrated to 40% moisture content produced CSB with the best texture and crumb structure, and the highest total score. Bran hydration merely induced negative effects on smoothness of noodles made with fine bran. Autoclaving of bran significantly improved texture and crumb structure of CSB made from WWFs with bran hydrated to 40% moisture. Autoclaved bran with 20% moisture contributed to higher total score of CSB. However, bran hydration and autoclaving resulted in darker color of noodle sheet made from WWFs.ConclusionsHydration and autoclaving of bran could be an effective way to improve the processing quality of CSB made from WWFs, but there was little improvement in eating quality of noodles made from WWFs.Significance and noveltyThis is the first research to show that hydration and autoclaving of bran could be an effective way to improve the processing quality of CSB made from WWFs. These results provide useful information for the incorporation of wheat bran to make fiber‐rich CSB.
On Water Cu@g‐C3N4 Catalyzed Synthesis of NH‐1,2,3‐Triazoles via [2+3] Cycloadditions of Nitroolefins/Alkynes and Sodium Azide
Authors: Payra Soumen , Saha Arijit , Banerjee Subhash 
Published: October 2018

AbstractHere, we have reported fabrication of graphitic polymeric C3N4 supported CuCl2 (Cu@g‐C3N4) and characterized by powder X‐ray diffraction, field emission scanning electron microscopy, high resolution transmission electron microscopy, X‐ray photoelectron spectroscopy studies. An efficient and regioselective protocol for the on water synthesis of 4‐aryl‐NH‐1,2,3‐triazole derivatives via 1,3‐diipolar cycloaddition reactions of nitroolefins/phenylacetylenes to sodium azide were demonstrated by using Cu@g‐C3N4 as robust and reusable catalyst.
Polyelectrolytes Tailored Enzyme Cascades with Enhanced Stability and Activity for One‐pot Synthesis
Authors: Wang Zheyu , Wang Junqian , Chen Gong , Xu Weina , Fu Zhongwang , Jiang Guoqiang , Wu Jianzhong , Liu Zheng 
Published: October 2018

AbstractCascade enzymes from the same origin or different resources often differ in their pH windows of stability and activity, thus impeding one‐pot enzyme cascade reactions. Here we present a method to assemble enzyme cascades using enzyme‐polyelectrolyte as the building block, in which the polyelectrolytes not only assemble enzymatic cascades via electrostatic interactions but also create favorable microenvironments for enzymes to expand their pH windows of stability and activity. We first demonstrated the effectiveness of this method for the oxidization of p‐xylene to p‐toluic acid using chloroperoxidase (CPO) and xanthine oxidase (XO) cascade, in which CPO from Caldariomyces fumago and XO from bovine milk were covalently linked to polymethacrylic acid (PMAA) and chitosan quaternary ammonium salt, respectively. Conjugation of the enzymes with PMAA and chitosan expanded the stability and activity pH windows for CPO and XO and increased the yield of p‐toluic acid production from p‐xylene by 7.6‐fold in comparison to that obtained from their native counterparts in soluble form. We then applied this method to assemble glucose oxidase (GOx) and horseradish peroxidase (HRP) cascade. The GOx‐PMAA@HRP‐chitosan displayed a lower Km and higher apparent activity at low glucose concentration, as compared to GOx/HRP cascade in soluble form, suggesting that the assembly method boosted the outcome of the one‐pot enzyme cascade reaction.
Puzzling Reaction of Imidazole with Methyl Parathion: P=S versus P=O Mechanistic Shift Dilemma in Organophosphates
Authors: Silva Valmir B. , Nascimento Leonardo L. Q. , Nunes Marcelo C. , Campos Renan B. , Oliveira Alfredo R. M. , Orth Elisa S. 
Published: October 2018

AbstractOrganophosphates (OPs) constitute many toxic agrochemicals and warfare and can undergo a wide spectrum of mechanisms, some which are fairly unexplored. In this sense, concise mechanistic elucidation stands out as a strategic tool for achieving efficient detoxification and for monitoring processes. Particularly intriguing is the effect of substituting the oxygen atom of the phosphoryl moiety (P=O) in OPs with a sulfur atom to give the thio‐derived OPs (i.e., OTPs, P=S). In general, imidazole (IMZ) reacts very efficiently with OPs by targeting the phosphorus atom, although herein we evidence a thio‐driven shift with OTPs: IMZ undergoes unusual nucleophilic attack at the aliphatic carbon atom of methyl parathion. Alkylation of IMZ under mild conditions (aqueous weakly basic medium) is also novel and should be applicable to other novel IMZ‐based architectures, and thereby, it can be a great ally for organic synthesis. Overall, a broader understanding of the mechanistic trend involved in such highly toxic agents is provided.
Intrinsic dissolution kinetics and topochemistry of xylan, mannan, and lignin during auto‐hydrolysis of red maple wood meal
Authors: Jara Rory , Lawoko Martin , van Heiningen Adriaan 
Published: October 2018

AbstractHigh temperature aqueous treatment of wood is the preferred technology for deconstructing lignocellulosics. Many studies have been carried out on the kinetics and mechanism of hot‐water extraction. However, most were performed in batch or integral plug flow reactors, which are not optimal for measuring intrinsic dissolution kinetics of the lignocellulosic components. Therefore, we used a continuous mixed batch reactor (or Berty reactor) to determine the intrinsic dissolution kinetics of xylan, mannan, and lignin from milled hardwood (Acer rubrum) at three different temperatures (150, 160, and 170 °C) and four constant pH values: 2, 3, 4, and 5. During the initial phase of autohydrolysis (carbohydrate‐free), lignin and (lignin‐free) xylan dissolve starting at a high rate and then a slowly decreasing rate, respectively. This is followed by the dissolution of xylan‐lignin complexes and finally cellulose xylan complexes when cellulose has been significantly hydrolysed. The kinetics and molecular weight distribution of the removed wood polymers are used to describe the topochemistry of autohydrolysis based on recent knowledge of the ultrastructure of hardwood fibres.
Redispersible Gold Nanoparticle/Polymer Composite Powders Ready for Ligand Exchange Reactions
Authors: Kronawitt Julia , Fan Ziyin , Schöttle Marius , Agarwal Seema , Greiner Andreas 
Published: October 2018

AbstractDry powders embedded with gold nanoparticles (AuNPs) are produced by spray‐drying technique from an aqueous AuNP dispersion in a poly(vinyl alcohol) (PVA) or poly(vinyl pyrrolidone) (PVP) matrix. The prepared powders consist of AuNPs stabilized in hollow capsules of PVA (PVA@AuNP) or PVP (PVP@AuNP) with an average diameter of several micrometers. The AuNP powders are dispersible in water and no agglomeration triggered by the spray drying process of AuNPs is observed. The PVA@AuNP powders are coextruded with polycaprolactone to demonstrate the industrial application of dry‐state AuNPs. With PVP@AuNP the incorporation of AuNP into other stabilizing polymers via ligand exchange method is facilitated. After each step, the active surface of the AuNPs was tested by catalytic studies.
A spectroscopic method for quick evaluation of tint strength and tint tone of titania (rutile) pigment and factors affecting them
Authors: Sharafudeen Riyas 
Published: October 2018

AbstractTitanium dioxide (TiO2) manufacturing industries measure optical properties such as brightness, colour, tint strength, tint tone, gloss, scatter coefficient, and others to ensure the quality of the product. Product characterization and process control generally focus on the optical properties, which determine its quality. In this work, titania rutile pigment with varying tint strength and tint tone is analyzed and a correlation is established between particle size and the optical properties such as tint strength and tint tone. It is observed that optical properties of titania pigment depends on the particle size as well as particle size distribution. A relatively faster evaluation of tint strength and tint tone can be made using the reflectance and particle size measurements. Analytical samples were prepared by ultrasonic dispersion of pigment in water containing calgon as dispersing agent. To verify the accuracy of the method, pigment tint strength and tint tone obtained from this study are compared with those resulting from traditional analysis. The results showed that the new method is viable.
Highly Efficient CRISPR‐Cas9‐Mediated Genome Editing in Human Pluripotent Stem Cells
Authors: Maguire Jean Ann , Cardenas‐Diaz Fabian L. , Gadue Paul , French Deborah L. 
Published: October 2018

AbstractHuman PSCs offer tremendous potential for both basic biology and cell‐based therapies for a wide variety of diseases. The ability to manipulate the genome of these cells using the CRISPR‐Cas9 technology has expanded this potential by providing a valuable tool for engineering or correcting disease‐associated mutations. Because of the high efficiency with which CRISPR‐Cas9 creates targeted double‐strand breaks, a major challenge has been the introduction of precise genetic modifications on one allele, without indel formation on the non‐targeted allele. To overcome this obstacle, we describe the use of two oligonucleotides, one expressing the sequence change, with the other maintaining the normal sequence. In addition, we have streamlined both the transfection and screening methodology to make this protocol efficient with small numbers of cells and to limit the amount of labor‐intensive clone passaging. This protocol provides a streamlined and technically simple approach for generating valuable tools to model human disease in stem cells. © 2018 by John Wiley & Sons, Inc.
Endoscopic ultrasound‐guided fine needle aspiration with liquid‐based cytology preparation in the diagnosis of metastatic small‐cell carcinoma in the pancreas
Authors: Son Jun Hyuk , Park Hye Kyeong , Kim Han‐Seong , Kim Nam‐Hoon , Kim Jung Wook , Bae Won Ki , Kim Kyung‐Ah , Lee June Sung , Lee Yoon Suk 
Published: October 2018

AbstractPancreatic metastasis is extremely rare, particularly from small‐cell lung cancer (SCLC). Studies on the role of endoscopic ultrasound‐guided fine needle aspiration (EUS‐FNA) with liquid‐based cytology (LBC) in the diagnosis of metastatic small‐cell carcinoma in the pancreas have been rarely conducted. We report herein a case of pancreatic metastasis from SCLC diagnosed using EUS‐FNA with LBC (ThinPrep). A 71‐year‐old man presented with chief complaints of hemoptysis and jaundice over the past 1 month. Lung & pancreas tumors with multiple liver nodules were detected on computed tomography. The aspirated material from the pancreas using EUS‐FNA was prepared as a cytologic specimen with ThinPrep method, which revealed scattered and clustered “small blue cells” with scant cytoplasm and stippled chromatin with frequent apoptotic bodies. Immunocytochemical staining of the cellblock material revealed strong positivity for CD56 and thyroid transcription factor‐1. Endobronchial biopsy for lung mass revealed nests of small, round, blue tumor cells with hyperchromatic nuclei showing salt and pepper chromatin, scant cytoplasm, and brisk mitotic activity. Therefore, a diagnosis of metastatic small‐cell carcinoma to the pancreas with an extensive stage was finally made.
Synergy of cytological methods in the pathological staging of breast cancer: Axillary fine‐needle aspiration and intraoperative scrape cytology of the sentinel lymph node
Authors: Bruzzone Martina , Saro Francesca , Bruno Sara , Celiento Tiziana , Mazzarella Giovanna , Lanata Sergio , Aquilano Maria Costanza , Parmigiani Gianpietro , Pollone Massimo , Gandolfo Fabrizio , Costigliolo Giovanni , Sironi Maria 
Published: October 2018

AbstractBackgroundSentinel lymph node biopsy (SLNB) has become the standard in breast cancer staging, but it is costly and time‐consuming. Fine‐needle aspiration cytology (FNAC) under ultrasonographic guidance identifies patients who need axillary lymph‐node dissection (ALND), thus reducing costs. As an alternative to frozen sections (FS), intraoperative scrape cytology (ISC) for SLNB is an inexpensive, rapid, accurate and safe technique. We evaluated the synergy of FNAC and SLNB in determining the axillary burden and the performance of the ISC method.MethodsOver a nine‐year period, 894 breast cancer patients were analyzed. Of these, 439 patients with echographic suspicious nodes underwent preoperative FNAC; negative axillary ultrasounds or FNACs resulted in 606 intraoperative SLNB, performed using the ISC technique. The results were compared with histological diagnosis, and sensitivity, specificity, predictive values and accuracy were calculated.ResultsOf the 439 FNACs, 121 were positive and underwent immediate ALND, and 242 negative patients underwent intraoperative SLNB (69% sensitivity, 99% specificity). Positive cases often had multiple nodal involvement (55% pN2‐3). Of the 606 SLNB‐ISC smears, 510 were true negative; 65 true positives allowed for one‐step ALND (71% sensitivity, 99% specificity).ConclusionPreoperative positive axillary FNAC predicts a higher disease burden and determines the avoidance of SLNB for patients eligible for immediate ALND. ISC instead of FS is a safe and sensitive technique to identify metastases, indicating completion of ALND.Partially presented at

Joint International Oncology (sentinel node & cancer metastasis) Congress, May 27‐29, 2013, San Francisco, California, USA
18 ° International Congress of Cytology (ICC 2013‐1161), May 26‐30, 2013, Paris, France
Convegno Nazionale GISMa ‐ Finalborgo (Savona), Italy,19‐20 maggio 2016
Fine‐needle aspiration features of extraskeletal myxoid chondrosarcoma: A study of cytological and molecular features
Authors: Santos Filipa , Martins Carmo , Lemos Maria M. 
Published: October 2018

BackgroundExtraskeletal myxoid chondrosarcoma (EMC) is a tumor of uncertain differentiation. Few data are available regarding its cytomorphological features in fine‐needle aspiration (FNA). Specific cytogenetic alterations involving the NR4A3 gene are found in EMC and can be identified in FNA samples.MethodsWe retrospectively reviewed 14 FNAs performed in 11 patients with an EMC; 10 FNAs were performed preoperatively on primary tumors; 2 were performed on recurrences and 2 were performed on metastasis. Cytological features were compared with histological findings. Immunohistochemistry (IHC) and molecular studies were performed both in FNA and histological specimens.ResultsA preoperative cytological diagnosis of EMC was rendered in eight FNAs performed in newly diagnosed tumors and in recurrent/metastatic cases. A descriptive diagnosis of a myxoid neoplasm was made in the remaining two cases. Smears were moderately hypercellular, composed of plasmocytoid to fusiform cells, with scant, pale cytoplasm, bland nuclei and inconspicuous nucleoli, dispersed as cords, strands and occasionally with a lace‐like pattern, in an abundant chondromyxoid matrix. IHC performed showed focal positivity for S100 and NSE. Fluorescence in situ hybridization technique performed in three FNA specimens showed EWSR1 gene rearrangements in all, concomitant with NR4A3 gene rearrangement in one case. Histological specimens showed typical features of EMC and NR4A3 gene rearrangements were found in all cases tested.ConclusionFNA cytology is a reliable method to perform a preoperative diagnosis of EMC regarding its cytomorphological and molecular features. Main differential diagnoses include myoepithelial tumors of soft tissue, myxoid liposarcoma and myxofibrosarcoma. Ancillary studies are helpful when coupled with cytomorphology evaluation.
Sex differences in the nicotinic excitation of principal neurons within the developing hippocampal formation
Authors: Chung Beryl Y.T. , Bailey Craig D.C. 
Published: October 2018

AbstractThe hippocampal formation (HF) plays an important role to facilitate higher order cognitive functions. Cholinergic activation of heteromeric nicotinic acetylcholine receptors (nAChRs) within the HF is critical for the normal development of principal neurons within this brain region. However, previous research investigating the expression and function of heteromeric nAChRs in principal neurons of the HF is limited to males or does not differentiate between the sexes. We used whole‐cell electrophysiology to show that principal neurons in the CA1 region of the female mouse HF are excited by heteromeric nAChRs throughout postnatal development, with the greatest response occurring during the first two weeks of postnatal life. Excitability responses to heteromeric nAChR stimulation were also found in principal neurons in the CA3, dentate gyrus, subiculum, and entorhinal cortex layer VI (ECVI) of young postnatal female HF. A direct comparison between male and female mice found that principal neurons in ECVI display greater heteromeric nicotinic passive and active excitability responses in females. This sex difference is likely influenced by the generally more excitable nature of ECVI neurons from female mice, which display a higher resting membrane potential, greater input resistance, and smaller afterhyperpolarization potential of medium duration (mAHP). These findings demonstrate that heteromeric nicotinic excitation of ECVI neurons differs between male and female mice during a period of major circuitry development within the HF, which may have mechanistic implications for known sex differences in the development and function of this cognitive brain region.
Pooled whole‐genome sequencing of interspecific chestnut (Castanea) hybrids reveals loci associated with differences in caching behavior of fox squirrels (Sciurus niger L.)
Authors: LaBonte Nicholas R. , Woeste Keith E. 
Published: October 2018

AbstractDispersal of seeds by scatter‐hoarding rodents is common among tropical and temperate tree species, including chestnuts in the genus Castanea. Backcrossed (BC) interspecific hybrid chestnuts exhibit wide variation in seed traits: as the parent species (Castanea dentata and C. mollissima) have distinct seed phenotypes and tend to be handled differently by seed dispersers, phenotypic variation in BC trees is likely due to inheritance of genes that have undergone divergent evolution in the parent species. To identify candidate genomic regions for interspecific differences in seed dispersal, we used tagged seeds to measure average dispersal distance for seeds of third‐generation BC chestnuts and sequenced pooled whole genomes of mother trees with contrasting seed dispersal: high caching rate/long distance; low caching rate/short distance; no caching. Candidate regions affecting seed dispersal were identified as loci with more C. mollissima alleles in the high caching rate/ long‐distance pool than expected by chance and observed in the other two pools. Functional annotations of candidate regions included predicted lipid metabolism, dormancy regulation, seed development, and carbohydrate metabolism genes. The results support the hypothesis that perception of seed dormancy is a predominant factor in squirrel caching decisions, and also indicate profitable directions for future work on the evolutionary genomics of trees and coevolved seed dispersers.