bluuebunny/crossref_metadata_2025_split huggingface dataset:
How Systems Analysis, Cost-Effectiveness Analysis, or Benefit-Cost Analysis First Became Influential in Federal Government Program Decision-Making
Authors: Enthoven Alain 
Published: August 2019

AbstractIn 1948, the RAND Corporation, formed to connect military planning with research and development decisions, became an independent nonprofit organization. Before then, cost-effectiveness analysis, benefit-cost analysis, and systems analysis had no established home in the federal government. In the 1950s, under the leadership of Charles Hitch, Chief, RAND Economics, undertook a program of activities they called “systems analysis,” including evaluation of the costs and effectiveness of weapon systems. In 1961, Robert McNamara appointed Hitch to be the Comptroller of the Department of Defense and invited Hitch to carry out his vision he described as “Programming and Systems Analysis.” Programming became the Planning, Programming, Budgeting System (PPBS) and the Five-Year Defense Program that linked strategies to forces to budgets. Systems Analysis assisted the Secretary to make choices of weapon systems and strategies. In 1965, Hitch returned to California and ultimately became President of the university. McNamara wanted Systems Analysis to report directly to him, and on his recommendation, President Lyndon Johnson appointed me Assistant Secretary for Systems Analysis. In 1966, the President directed that all departments in the executive branch establish offices based on the Systems Analysis model. In 1967, Henry S. Rowen became President of the RAND Corporation. He broadened RAND’s scope beyond the military to include Health Services, education, urban problems including homelessness, ethics in scientific research, and climate research. In 1970, Rowen led the establishment of the Pardee RAND Graduate School, offering a doctoral degree in Public Policy Analysis to extend widely the application of the RAND Systems Analysis approach to many fields.
Settling the Citizen, Settling the Nomad: ‘Habitual offenders’, rebellion, and civic consciousness in western India, 1938–1952
Authors: BAJRANGE DAKXINKUMAR , GANDEE SARAH , GOULD WILLIAM 
Published: August 2019

AbstractThis article explores the politics of civic engagement during India's long decolonization between 1938 and 1952 for communities—the erstwhile ‘criminal tribes’—whose lifestyles were complicated by controls on their movement before and shortly following India's independence. It argues that their varied and contingent strategies of mobilization increasingly identified community particularities—notably, their marking as ‘criminals’ and a history of movement—as a basis for negotiating their problematic inclusion within the evolving citizenship frameworks of the late colonial, then post-colonial, state. These early forms of civic consciousness set the parameters for later strategies that sought to mobilize communities by engaging with ‘universal’, ‘differentiated’, and indigenized conceptions of civic responsibility and rights. The most surprising finding of this research is that these strategies (via anti-colonialism) often embraced and celebrated forms of illegality and criminality. The romanticism of the dacoit (bandit)-cum-freedom fighter charged Dhaku Sultan-like figures with political heroism. In the context of independence and the founding of the Constitution, strategies turned to the (un)realized promises of freedom and citizenship rights. The final part of the article turns to the implications of ‘denotification’ for the so-called criminal tribes in the early 1950s, which provided both obstacles and avenues to strategies of mobilization after independence.
Biogeographically significant units in conservation: a new integrative concept for conserving ecological and evolutionary processes
Authors: Quiroga M Paula , Castello Lucia , Quipildor Vilma , Premoli Andrea C 
Published: August 2019

SummaryWe combined tools of phylogeography, population genetics and biogeographical interpretation to analyse a group of phylogenetically independent lineages (animals and plants) that coexist within the same geographical region, yet under markedly different environments, in order to identify generalized barriers for gene flow. We tested the hypothesis that major geographic features have produced a concordant genetic structure in phylogenetically independent lineages. A rigorous bibliographic search was performed, selecting available molecular information from six taxa occupying distinct southern biomes of South America: Yungas, Prepuna, Puna and northern Monte. We estimated within-population genetic diversity, the genetic structure and haplotype phylogenies to assemble distribution maps of genetic barriers for each species. We found a strong association between genetic variation and latitudinal distribution of populations. We detected a major barrier for six taxa at 27°S latitude and a second one for a group of three species at 25–26°S. Two alternative non-exclusive hypotheses – geology and/or climate – explain concordant genetic barriers in divergent lineages. We suggest that the term ‘biogeographically significant units’ portrays a group of populations of phylogenetically unrelated taxa that inhabit the same geographic region that have been similarly impacted by major physical events, which can be used to identify priority areas in landscape conservation.
Seroprevalence of HPV serotypes 6, 11, 16 and 18 in unvaccinated children from Mexico City
Authors: Pacheco-Domínguez Reyna Lizette , Durazo-Arvizu Ramón A. , López-Hernández Angélica , Figueroa-Padilla Jesica , Ramírez-González Julia Berenice , López-Cervantes Malaquías 
Published: August 2019

Abstract
Data regarding humoral immunity against HPV infection are scarce. Most analyses focus on the identification of viruses on mucous membranes and primarily refer to women of reproductive age. The aim of this work was to estimate the seroprevalence of antibodies against HPV serotypes 6, 11, 16 and 18 among unvaccinated boys living in Mexico City. A cross-sectional study of 257 male students from 48 public primary schools in Mexico City, whose ages fluctuated between 9 and 14 years, was carried out. Immunological status was assessed by applying the competitive Luminex Immunoassay of HPV (cLIA). Among the study population, we initially found that 38.52% (n = 99) of the children tested positive against one or more of the HPV 6, 11, 16 and/or 18 serotypes. The most commonly found serotype was isolated HPV 18 or in combination with other serotypes (22% and 31%, respectively), followed by HPV 6 with frequencies of 4.7% and 11%, respectively; however, lower frequencies were estimated for HPV 16 (2%; 6%) and isolated HPV 11, 4%. If a second set of cut-off points for seropositivity is applied, the overall prevalence for any serotype is reduced to 15.2%. As it appears that a significant sector of the study population has had basal contact with an HPV serotype, we recommend considering the possibility of vaccination against HPV at earlier ages.
Inflammation throughout pregnancy and fetal growth restriction in rural Nepal
Authors: Sauder Michael W. , Lee Sun Eun , Schulze Kerry J. , Christian Parul , Wu Lee S. F. , Khatry Subarna K. , LeClerq Steven C. , Adhikari Ramesh K. , Groopman John D. , West Keith P. 
Published: August 2019

AbstractMaternal systemic inflammation during pregnancy may restrict embryo−fetal growth, but the extent of this effect remains poorly established in undernourished populations. In a cohort of 653 maternal−newborn dyads participating in a multi-armed, micronutrient supplementation trial in southern Nepal, we investigated associations between maternal inflammation, assessed by serumα1-acid glycoprotein and C-reactive protein, in the first and third trimesters of pregnancy, and newborn weight, length and head and chest circumferences. Median (IQR) maternal concentrations inα1-acid glycoprotein and C-reactive protein in the first and third trimesters were 0.65 (0.53–0.76) and 0.40 (0.33–0.50) g/l, and 0.56 (0.25–1.54) and 1.07 (0.43–2.32) mg/l, respectively.α1-acid glycoprotein was inversely associated with birth size: weight, length, head circumference and chest circumference were lower by 116 g (P= 2.3 × 10−6), and 0.45 (P= 3.1 × 10−5), 0.18 (P= 0.0191) and 0.48 (P= 1.7 × 10−7) cm, respectively, per 50% increase inα1-acid glycoprotein averaged across both trimesters. Adjustment for maternal age, parity, gestational age, nutritional and socio-economic status and daily micronutrient supplementation failed to alter any association. Serum C-reactive protein concentration was largely unassociated with newborn size. In rural Nepal, birth size was inversely associated with low-grade, chronic inflammation during pregnancy as indicated by serumα1-acid glycoprotein.
Antibiotic-resistant pathogens in different patient settings and identification of surveillance gaps in Switzerland – a systematic review
Authors: Fulchini R. , Albrich W. C. , Kronenberg A. , Egli A. , Kahlert C. R. , Schlegel M. , Kohler P. 
Published: August 2019

AbstractThe prevalence of antimicrobial resistance (AMR) varies significantly among different patient populations. We aimed to summarise AMR prevalence data from screening studies in different patient settings in Switzerland and to identify surveillance gaps. We performed a systematic review, searching Pubmed, MEDLINE, Embase (01/2000–05/2017) and conference proceedings for Swiss studies reporting on carbapenemase-producingEnterobacteriaceae(CPE), extended-spectrum beta-lactamases (ESBL), mobilised colistin-resistance, methicillin-resistantStaphylococcus aureus(MRSA) and vancomycin-resistantEnterococci(VRE) within different patient settings. We identified 2345 references and included 46 studies. For acute care patients, most screening data come from admission screenings, whereas AMR prevalence among hospitalised patients is largely unknown. Universal admission screenings showed ESBL-prevalences of 5–8% and MRSA-prevalences of 2–5%. For targeted screening, ESBL-prevalence ranged from 14–21%; MRSA-prevalence from 1–4%. For refugees, high ESBL (9–24%) and MRSA (16–24%) carriage rates were reported; returning travellers were frequently (68–80%) colonised with ESBL. Screening data for other pathogens, long-term care facility (LTCF) residents and pediatric populations were scarce. This review confirms high ESBL- and MRSA-carriage rates for risk populations in Switzerland. Emerging pathogens (CPE and VRE) and certain populations (inpatients, LTCF residents and children) are understudied. We encourage epidemiologists and public health authorities to consider these findings in the planning of future surveillance studies.
Anxiety, depression, and behavioural rating scales in children with non-cardiac chest pain
Authors: Kenar Aytaç , Örün Utku Arman , Yoldaş Tamer , Kayalı Şeyma , Bodur Şahin , Karademir Selmin 
Published: August 2019

AbstractIntroduction:Chest pain is the second most common reason for referral to paediatric cardiologists after benign heart murmurs. Aetiology frequently depends on non-cardiac reasons. In addition, individuals may experience non-cardiac chest pain which is idiopathic or of unknown origin. The aim of this study is to examine psychological symptoms in children and adolescents with medically unexplained chest pain.Methods:A total of 76 patients (ages 8–18 years) were included in the study, who were referred to the paediatric cardiology department with the complaint of chest pain but did not have any detected cardiac aetiology or any other organic causes of chest pain. The control group was composed of 51 healthy volunteers. Self-evaluation scales were given to both groups which included Beck Anxiety Inventory and Children’s Depression Inventory. Also parents of both groups completed the Conner’s Parent Rating Scale for assessment of Attention-deficit/hyperactivity disorder.Results:Anxiety scores of the non-cardiac chest pain group were significantly higher compared to controls. No significant differences were found between patients and controls in terms of attention-deficit/hyperactivity disorder and depression scores. In patient group, patterns were similar for boys and girls and for children and adolescents; except girls scored significantly higher than boys in children’s depression inventory.Conclusions:In children and adolescents, non-cardiac chest pain is associated with increased levels of anxiety. These results show the importance of psychiatric evaluation in non-cardiac chest pain patients. Larger controlled studies are needed to determine the prevalence and impact of attention-deficit/hyperactivity disorder and depression in children and adolescents with non-cardiac chest pain.
Seismo‐Mechanical Response of Anisotropic Rocks Under Hydraulic Fracture Conditions: New Experimental Insights
Authors: Gehne S. , Benson P. M. , Koor N. , Dobson K. J. , Enfield M. , Barber A. 
Published: August 2019

AbstractUnconventional hydrocarbon resources found across the world are driving a renewed interest in mudrock hydraulic fracturing methods. However, given the difficulty in safely measuring the various controlling factors in a natural environment, considerable challenges remain in understanding the fracture process. To investigate, we report a new laboratory study that simulates hydraulic fracturing using a conventional triaxial apparatus. We show that fracture orientation is primarily controlled by external stress conditions and the inherent rock anisotropy and fabric are critical in governing fracture initiation, propagation, and geometry. We use anisotropic Nash Point Shale (NPS) from the early Jurassic with high elastic P wave anisotropy (56%) and mechanical tensile anisotropy (60%), and highly anisotropic (cemented) Crab Orchard Sandstone with P wave/tensile anisotropies of 12% and 14%, respectively. Initiation of tensile fracture requires 36 MPa for NPS at 1‐km simulated depth and 32 MPa for Crab Orchard Sandstone, in both cases with cross‐bedding favorable orientated. When unfavorably orientated, this increases to 58 MPa for NPS at 800‐m simulated depth, far higher as fractures must now traverse cross‐bedding. We record a swarm of acoustic emission activity, which exhibits spectral power peaks at 600 and 100 kHz suggesting primary fracture and fluid‐rock resonance, respectively. The onset of the acoustic emission data precedes the dynamic instability of the fracture by 0.02 s, which scales to ~20 s for ~100‐m size fractures. We conclude that a monitoring system could become not only a forecasting tool but also a means to control the fracking process to prevent avoidable seismic events.
Large‐Amplitude Mountain Waves in the Mesosphere Observed on 21 June 2014 During DEEPWAVE: 2. Nonlinear Dynamics, Wave Breaking, and Instabilities
Authors: Fritts David C. , Wang Ling , Taylor Michael J. , Pautet Pierre‐Dominique , Criddle Neal R. , Kaifler Bernd , Eckermann Stephen D. , Liley Ben 
Published: August 2019

AbstractWeak cross‐mountain flow over the New Zealand South Island on 21 June 2014 during the Deep Propagating Gravity Wave Experiment (DEEPWAVE) led to large‐amplitude mountain waves in the mesosphere and lower thermosphere. The mesosphere and lower thermosphere responses were observed by ground‐based instruments in the lee of the Southern Alps supporting DEEPWAVE, including an Advanced Mesosphere Temperature Mapper, a Rayleigh lidar, an All‐Sky Imager, and a Fabry‐Perot Interferometer. The character of the mountain wave responses at horizontal scales of ~30–90 km reveals strong “sawtooth” variations in the temperature field suggesting large vertical and horizontal displacements leading to mountain wave overturning. The observations also reveal multiple examples of apparent instability structures within the mountain wave field that arose accompanying large amplitudes and exhibited various forms, scales, and evolutions. This paper employs detailed data analyses and results of numerical modeling of gravity wave instability dynamics to interpret these mountain wave dynamics, their instability forms, scales, and expected environmental influences. Results demonstrate apparently general instability pathways for breaking of large‐amplitude gravity waves in environments without and with mean shear. A close link is also found between large‐amplitude gravity waves and the dominant instability scales that may yield additional abilities to quantify gravity wave characteristics and effects.
Interface Flow With Vertically Varying Hydraulic Conductivity
Authors: Toller Erik A. L. , Strack Otto D. L. 
Published: August 2019

AbstractWe present an approach for solving problems of saltwater intrusion in aquifers with an hydraulic conductivity that varies in the vertical direction. The approach is based on the concept of a comprehensive potential, whose gradient exactly represents the vertically integrated discharge, which is, by definition, a two‐dimensional vector. We apply the approach to an island in Sweden and use it to estimate maximum allowable pumping rates of wells that recover part of the infiltration on the island.
Sex pilus specific bacteriophage to drive bacterial population towards antibiotic sensitivity
Authors: Colom Joan , Batista Diego , Baig Abiyad , Tang Ying , Liu Siyang , Yuan Fangzhong , Belkhiri Aouatif , Marcelino Lucas , Barbosa Fernanda , Rubio Marcela , Atterbury Robert , Berchieri Angelo , Barrow Paul 
Published: August 2019

AbstractAntimicrobial resistance (AMR) is now a major global problem largely resulting from the overuse of antibiotics in humans and livestock. In some AMR bacteria, resistance is encoded by conjugative plasmids expressing sex-pili that can readily spread resistance through bacterial populations. The aim of this study was to use sex pilus-specific (SPS) phage to reduce the carriage of AMR plasmids. Here, we demonstrate that SPS phage can kill AMR Escherichia coli and select for AMR plasmid loss in vitro. For the first time, we also demonstrate that SPS phage can both prevent the spread of AMR Salmonella Enteritidis infection in chickens and shift the bacterial population towards antibiotic sensitivity.
Accurate Classification of Biological and non-Biological Interfaces in Protein Crystal Structures using Subtle Covariation Signals
Authors: Fukasawa Yoshinori , Tomii Kentaro 
Published: August 2019

AbstractProteins often work as oligomers or multimers in vivo. Therefore, elucidating their oligomeric or multimeric form (quaternary structure) is crucially important to ascertain their function. X-ray crystal structures of numerous proteins have been accumulated, providing information related to their biological units. Extracting information of biological units from protein crystal structures represents a meaningful task for modern biology. Nevertheless, although many methods have been proposed for identifying biological units appearing in protein crystal structures, it is difficult to distinguish biological protein–protein interfaces from crystallographic ones. Therefore, our simple but highly accurate classifier was developed to infer biological units in protein crystal structures using large amounts of protein sequence information and a modern contact prediction method to exploit covariation signals (CSs) in proteins. We demonstrate that our proposed method is promising even for weak signals of biological interfaces. We also discuss the relation between classification accuracy and conservation of biological units, and illustrate how the selection of sequences included in multiple sequence alignments as sources for obtaining CSs affects the results. With increased amounts of sequence data, the proposed method is expected to become increasingly useful.
Association of Aggresomes with Survival Outcomes in Pediatric Medulloblastoma
Authors: Yehia Maha , Taha Hala , Salama Asmaa , Amer Nada , Mosaab Amal , Hassanain Omneya , Refaat Amal , Yassin Dina , El-Hemaly Ahmed , Ahmed Soha , El-Beltagy Mohamed , Shaalan Osama , El-Naggar Shahenda 
Published: August 2019

AbstractAggresomes are inclusion bodies for misfolded/aggregated proteins. Despite the role of misfolded/aggregated proteins in neurological disorders, their role in cancer pathogenesis is poorly defined. In the current study we aimed to investigate whether aggresomes-positivity could be used to improve the disease subclassification and prognosis prediction of pediatric medulloblastoma. Ninety three pediatric medulloblastoma tumor samples were retrospectively stratified into three molecular subgroups; WNT, SHH and non-WNT/non-SHH, using immunohistochemistry and Multiplex Ligation Probe Amplification. Formation of aggresomes were detected using immunohistochemistry. Overall survival (OS) and event-free survival (EFS) were determined according to risk stratification criteria. Multivariate Cox regression analyses were carried out to exclude confounders. Aggresomes formation was detected in 63.4% (n = 59/93) of samples. Aggresomes were non-randomly distributed among different molecular subgroups (P = 0.00002). Multivariate Cox model identified aggresomes’ percentage at ≥20% to be significantly correlated with patient outcome in both OS (HR = 3.419; 95% CI, 1.30–8.93; P = 0.01) and EFS (HR = 3; 95% CI, 1.19–7.53; P = 0.02). The presence of aggresomes in ≥20% of the tumor identified poor responders in standard risk patients; OS (P = 0.02) and EFS (P = 0.06), and significantly correlated with poor outcome in non-WNT/non-SHH molecular subgroup; OS (P = 0.0002) and EFS (P = 0.0004).
Cytotoxic activity of copper(ii), nickel(ii) and platinum(ii) thiosemicarbazone derivatives: interaction with DNA and the H2A histone peptide
Authors: Bisceglie Franco , Orsoni Nicolò , Pioli Marianna , Bonati Beatrice , Tarasconi Pieralberto , Rivetti Claudio , Amidani Davide , Montalbano Serena , Buschini Annamaria , Pelosi Giorgio 
Published: August 2019

Abstract
Metal complexes still represent promising pharmacological tools in the development of new anticancer drugs. Bis(citronellalthiosemicarbazonate)nickel(ii) is a metal compound extremely effective against leukemic and NCS cancer cell lines. Preliminary experiments performed with this compound and with its Cu(ii) and Pt(ii) analogues evidenced alterations, detectable by comet assay, in the DNA of treated U937 cells. In addition, [Cu(tcitr)2] and [Pt(tcitr)2] were also able to induce gene mutations and produce frameshift events. To gain further insights into the mechanism of action of these metal compounds, we carried out a multidisciplinary study to investigate whether their biological activity can be ascribed to the direct interaction with DNA or with chromatin. The DNA interaction was investigated by means of CD and UV-Vis spectroscopic techniques and by AFM, whereas the chromatin interaction was studied by analyzing the effects of the compounds on the structure of a peptide that mimicks the potential metal binding site in the “C-tail” region of histone H2A by means of NMR, CD, UV-Vis and MS. The intensities of the effects induced by the metal compounds on the peptide follow the order [Ni(tcitr)2] &gt; [Pt(tcitr)2] ≫ [Cu(tcitr)2]. From the AFM data, a remarkable DNA compaction was observed in the presence of [Pt(tcitr)2], while [Ni(tcitr)2] causes the formation of large interlaced DNA aggregates.