bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of the Spanish smoke-free laws on cigarette sales, 2000–2015: partial bans on smoking in public places failed and only a total tobacco ban worked
Authors: Pinilla Jaime , López-Valcárcel Beatriz G. , Negrín Miguel A. 
Published: July 2018

AbstractIn January 2006, the Spanish government enacted a tobacco control law that banned smoking in bars and restaurants, with exceptions depending on the floor space of the premises. In January 2011, further legislation in this area was adopted, removing these exceptions. We analyse the effect produced on cigarette sales by these two bans. We approach this problem using an interrupted time series analysis while accounting for the potential effects of autocorrelation and seasonality. The data source used was the official data on legal sales of tobacco in Spain, from January 2000 to December 2015 (excluding the Canary Islands and the autonomous cities of Ceuta and Melilla). As confounder variables, we use the log-transformed average prices for manufactured and hand-rolled cigarettes (or the average minimum excise tax as a proxy), and log-transformed real-household disposable income. The implementation of a total smoke-free ban in Spain was associated with an immediate reduction in cigarette sales between 9% to 11%. In contrast, in the period immediately following the partial ban, no such reduction was detected, beyond the trend already present. Our results indicate that, in Spain, partial bans on smoking in public places failed, and that only a total tobacco ban worked.
Israel and the Islamist Challenge: Old Dilemmas, New Approaches
Authors: Daoud Suheir Abu Oksa 
Published: July 2018

AbstractThis paper explains Israel's decision to outlaw the Islamic Movement Northern Faction in Israel (IMNF) and examines the methods and strategies adopted by the IMNF and its leaders that prompted the state's actions. Based on the British Defense Regulations from the British Mandate for Palestine, the State of Israel outlawed the IMNF on November 17, 2015, accusing the group of incitement, racism, and terrorism. Sheykh Kamal Khatib, former deputy leader of IMNF, declared that the IMNF had been a tool to serve the Islamic project and regardless of having been outlawed, the movement “would find a “thousand ways” to serve that project.’” I argue that the IMNF's shift in focus from the Palestinians to the larger Muslim community disrupted politics within Israel. Even so, Israel's policy change was based on political and personal calculations, rather than on national and regional security pressures.
Catholicity Without Leviathan: Stanley Hauerwas's Perspective on the Church as an Alternative Political Community
Authors: Untea Ionut 
Published: July 2018

AbstractThe article brings into focus a series of political arguments of Stanley Hauerwas's “theological politics” and argues that these arguments are in stark contrast with the theoretical perspective of a political rule by a god-like Leviathan, an image inherited in modern and contemporary political culture from the early modern English philosopher Thomas Hobbes. The first section focuses on Hauerwas's arguments regarding the political potential of the term “Catholicity” to represent an alternative to the coercive politics reinforced by the post-Enlightenment nation state. The second section proposes a reflection on the way the Church's Catholicity may be expressed politically without falling into the temptation of involving the Leviathan to sort out the issues generated by its diversity. The concluding section illustrates how Hauerwas uses his approach of a universal unity of Christians “without Leviathan” in his exhortation addressed to American Christians to say “no” to Donald Trump's version of communal unity that is rather based on “total allegiance” to the United States and on “repressive politics”.
“I Have Been Bullied at Workplace!”: A Qualitative Study on Italian Employees’ Stories
Authors: Caputo Andrea 
Published: July 2018

AbstractThe present study explored some common thematic domains which characterized workplace bullying stories of adult Italian participants. Emotional Text Analysis was performed on 28 workplace bullying stories in order to detect the main themes (cluster analysis) and latent factors (correspondence analysis) emerging in bullied workers’ narratives. Five thematic domains were detected in the textual corpus (p < .05, at 95% confidence level), which are respectively referred to as: organizational constraints (14.10%), treatment discrimination (10.04%), unjust accusations (30.77%), emotional abuse (14.53%), and job duty changes (30.56%). Four latent factors explained the overall data variance: Sense of precariousness (F1, 34.38% of variance), lack of recognition (F2, 26.35%), feeling of exclusion (F3, 23.01%) and job disengagement (F4, 16.27%). From the emotional experience shaping the relationship between bullied workers and their work environments, this research study allows the identification of some organizational situations that are “at risk” for workplace bullying in order to promote better organizational health.
Motorische Entwicklung und Händigkeit in den ersten beiden Lebensjahren
Authors: Krombholz Heinz 
Published: July 2018

Zusammenfassung. Der Zusammenhang von motorischer Entwicklung in den ersten beiden Lebensjahren und der Händigkeit von Kindern (N = 367) wurde untersucht. 18 motorische Meilensteine wurden erfasst, 14 betreffen die Grob- und vier die Handmotorik. Die Händigkeit wurde durch eine Befragung der Eltern zum Zeitpunkt des Erreichens des Meilensteins („Freies und sicheres Gehen“) ermittelt, den die Kinder im durchschnittlichen Alter von 14 Monaten bewältigten. 42.1 % der Kinder bevorzugten ihre rechte, 9.6 % ihre linke Hand, 32 % gebrauchten beide Hände gleich häufig. 16.4 % der Eltern konnten keine Angaben zur Händigkeit machen. Kinder, die bereits eine ausgeprägte Präferenz für die rechte Hand zeigten, bewältigten die meisten der Meilensteine früher als linkshändige und Kinder ohne Bevorzugung einer Hand. Kinder, deren Eltern keine Angabe zur Händigkeit machten, zeigten keine Nachteile gegenüber Rechtshändern. Unterschiede hinsichtlich der Entwicklung körperlicher Merkmale konnten zwischen den Gruppen nicht nachgewiesen werden. Der Anteil der Jungen unter den Linkshändern war deutlich höher als der der Mädchen. Mit Ausnahme des Meilensteins „Sitzen“ konnten keine Unterschiede im Erreichen der Meilensteine zwischen den Geschlechtern nachgewiesen werden.
Segmentation of the Main Himalayan Thrust Revealed by Low‐Temperature Thermochronometry in the Western Indian Himalaya
Authors: Eugster Patricia , Thiede Rasmus C. , Scherler Dirk , Stübner Konstanze , Sobel Edward R. , Strecker Manfred R. 
Published: July 2018

AbstractDespite remarkable tectonostratigraphic similarities along the Himalayan arc, pronounced topographic and exhumational variability exists in different morphotectonic segments. The processes responsible for this segmentation are debated. Of particular interest is a 30‐ to 40‐km‐wide orogen‐parallel belt of rapid exhumation that extends from central Nepal to the western Himalaya and its possible linkage to a midcrustal ramp in the basal décollement, and the related growth of Lesser Himalayan duplex structures. Here we present 26 new apatite fission track cooling ages from the Beas‐Lahul region, at the transition from the Central to the Western Himalaya (~77°–78°E) to investigate segmentation in the Himalayan arc from a thermochronologic perspective. Together with previously published data from this part of the orogen, we document significant lateral changes in exhumation between the Dhauladar Range to the west, the Beas‐Lahul region, and the Sutlej area to the east of the study area. In contrast to the Himalayan front farther east, exhumation in the far western sectors is focused at the frontal parts of the mountain range and associated with the hanging wall of the Main Boundary Thrust fault ramp. Our results allow us to spatially correlate the termination of the rapid exhumation belt with a midcrustal ramp to the west. We suggest that a plunging anticline at the northwestern edge of the Larji‐Kullu‐Rampur window represents the termination of the Central Himalayan segment, which is related to the evolution of the Lesser Himalayan duplex.
Early Jurassic Rifting of the Arabian Passive Continental Margin of the Neo‐Tethys. Field Evidence From the Lurestan Region of the Zagros Fold‐and‐Thrust Belt, Iran
Authors: Tavani Stefano , Parente Mariano , Vitale Stefano , Iannace Alessandro , Corradetti Amerigo , Bottini Cinzia , Morsalnejad Davoud , Mazzoli Stefano 
Published: July 2018

AbstractThe Arabian passive margin formed at the southern margin of the Neo‐Tethys ocean during the breakup of Pangea. In the Lurestan region of the Zagros mountain belt, the deformed Arabian continental paleo‐margin can be reconstructed as originally consisting of distinct crustal domains, including a proximal sector and a distal continental ribbon, separated by a deep‐water trough, known as the Radiolarite Basin. Such an architecture was shaped by the continental rifting process, thus reflecting timing and style of continent separation, which is generally assumed to have occurred during the Permo‐Triassic interval. This study reports evidence of syn‐sedimentary extensional faults, unconformities, and facies changes in the Mesozoic stratigraphic succession of the Lurestan region, which point to a major Jurassic extensional pulse. In detail, extension reached its climax at the end of the Early Jurassic, when tectonically driven drowning of the long‐lived Triassic to Early Jurassic carbonate platform led to the transition from shallow‐ to deep‐water environments in large areas of the inner margin, coevally with the development of the Radiolarite Basin. Our findings suggest a two‐step continental rifting in this area, with the first Permo‐Triassic phase predating an Early Jurassic one.
Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy
Authors: Liu Jing , Xiong Ranhua , Brans Toon , Lippens Saskia , Parthoens Eef , Zanacchi Francesca Cella , Magrassi Raffaella , Singh Santosh K. , Kurungot Sreekumar , Szunerits Sabine , Bové Hannelore , Ameloot Marcel , Fraire Juan C. , Teirlinck Eline , Samal Sangram Keshari , Rycke Riet De , Houthaeve Gaëlle , De Smedt Stefaan C. , Boukherroub Rabah , Braeckmans Kevin 
Published: July 2018

AbstractIn the replacement of genetic probes, there is increasing interest in labeling living cells with high-quality extrinsic labels, which avoid over-expression artifacts and are available in a wide spectral range. This calls for a broadly applicable technology that can deliver such labels unambiguously to the cytosol of living cells. Here, we demonstrate that nanoparticle-sensitized photoporation can be used to this end as an emerging intracellular delivery technique. We replace the traditionally used gold nanoparticles with graphene nanoparticles as photothermal sensitizers to permeabilize the cell membrane upon laser irradiation. We demonstrate that the enhanced thermal stability of graphene quantum dots allows the formation of multiple vapor nanobubbles upon irradiation with short laser pulses, allowing the delivery of a variety of extrinsic cell labels efficiently and homogeneously into live cells. We demonstrate high-quality time-lapse imaging with confocal, total internal reflection fluorescence (TIRF), and Airyscan super-resolution microscopy. As the entire procedure is readily compatible with fluorescence (super resolution) microscopy, photoporation with graphene quantum dots has the potential to become the long-awaited generic platform for controlled intracellular delivery of fluorescent labels for live-cell imaging.
Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish
Authors: Yang Dahai , Zheng Xin , Chen Shouwen , Wang Zhuang , Xu Wenting , Tan Jinchao , Hu Tianjian , Hou Mingyu , Wang Wenhui , Gu Zhaoyan , Wang Qiyao , Zhang Ruilin , Zhang Yuanxing , Liu Qin 
Published: July 2018

AbstractThe noncanonical inflammasome is critical for cytosolic sensing of Gram-negative pathogens. Here, we show that bacterial infection induces caspy2 activation in zebrafish fibroblasts, which mediates pyroptosis via a caspase-5-like activity. Zebrafish caspy2 binds directly to lipopolysaccharide via the N-terminal pyrin death domain, resulting in caspy2 oligomerization, which is critical for pyroptosis. Furthermore, we show that caspy2 is highly expressed in the zebrafish gut and is activated during infection. Knockdown of caspy2 expression impairs the ability of zebrafish to restrict bacterial invasion in vivo, and protects larvae from lethal sepsis. Collectively, our results identify a crucial event in the evolution of pattern recognition into the death domain superfamily-mediated intracellular lipopolysaccharide-sensing pathway in innate immunity.
Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative
Authors: Dai Zongjie , Huang Mingtao , Chen Yun , Siewers Verena , Nielsen Jens 
Published: July 2018

AbstractSaccharomyces cerevisiae is a Crabtree-positive eukaryal model organism. It is believed that the Crabtree effect has evolved as a competition mechanism by allowing for rapid growth and production of ethanol at aerobic glucose excess conditions. This inherent property of yeast metabolism and the multiple mechanisms underlying it require a global rewiring of the entire metabolic network to abolish the Crabtree effect. Through rational engineering of pyruvate metabolism combined with adaptive laboratory evolution (ALE), we demonstrate that it is possible to obtain such a global rewiring and hereby turn S. cerevisiae into a Crabtree-negative yeast. Using integrated systems biology analysis, we identify that the global rewiring of cellular metabolism is accomplished through a mutation in the RNA polymerase II mediator complex, which is also observed in cancer cells expressing the Warburg effect.
Uniaxial stress flips the natural quantization axis of a quantum dot for integrated quantum photonics
Authors: Yuan Xueyong , Weyhausen-Brinkmann Fritz , Martín-Sánchez Javier , Piredda Giovanni , Křápek Vlastimil , Huo Yongheng , Huang Huiying , Schimpf Christian , Schmidt Oliver G. , Edlinger Johannes , Bester Gabriel , Trotta Rinaldo , Rastelli Armando 
Published: July 2018

AbstractThe optical selection rules in epitaxial quantum dots are strongly influenced by the orientation of their natural quantization axis, which is usually parallel to the growth direction. This configuration is well suited for vertically emitting devices, but not for planar photonic circuits because of the poorly controlled orientation of the transition dipoles in the growth plane. Here we show that the quantization axis of gallium arsenide dots can be flipped into the growth plane via moderate in-plane uniaxial stress. By using piezoelectric strain-actuators featuring strain amplification, we study the evolution of the selection rules and excitonic fine structure in a regime, in which quantum confinement can be regarded as a perturbation compared to strain in determining the symmetry-properties of the system. The experimental and computational results suggest that uniaxial stress may be the right tool to obtain quantum-light sources with ideally oriented transition dipoles and enhanced oscillator strengths for integrated quantum photonics.
Automated single-molecule imaging in living cells
Authors: Yasui Masato , Hiroshima Michio , Kozuka Jun , Sako Yasushi , Ueda Masahiro 
Published: July 2018

AbstractAn automated single-molecule imaging system developed for live-cell analyses based on artificial intelligence-assisted microscopy is presented. All significant procedures, i.e., searching for cells suitable for observation, detecting in-focus positions, and performing image acquisition and single-molecule tracking, are fully automated, and numerous highly accurate, efficient, and reproducible single-molecule imaging experiments in living cells can be performed. Here, the apparatus is applied for single-molecule imaging and analysis of epidermal growth factor receptors (EGFRs) in 1600 cells in a 96-well plate within 1 day. Changes in the lateral mobility of EGFRs on the plasma membrane in response to various ligands and drug concentrations are clearly detected in individual cells, and several dynamic and pharmacological parameters are determined, including the diffusion coefficient, oligomer size, and half-maximal effective concentration (EC50). Automated single-molecule imaging for systematic cell signaling analyses is feasible and can be applied to single-molecule screening, thus extensively contributing to biological and pharmacological research.
Subjective time expansion with increased stimulation of intrinsically photosensitive retinal ganglion cells
Authors: Yang Pei-Ling , Tsujimura Sei-ichi , Matsumoto Akiko , Yamashita Wakayo , Yeh Su-Ling 
Published: July 2018

AbstractIntrinsically photosensitive retinal ganglion cells (ipRGCs) contain photoreceptors that are especially sensitive to blue light. Nevertheless, how blue light and ipRGCs affect time perception remains unsolved. We used the oddball paradigm and manipulated the background light to examine whether and how blue light and ipRGCs affect perceived duration. In the oddball paradigm, participants were asked to judge the duration of the target (oddball), compared to that of the standard, with a two alternative-forced-choice procedure. When the background light was controlled to be either blue or red in Experiment 1, results showed that blue light led to longer subjective duration compared to red light. Experiment 2 further clarified the contribution of the ipRGCs. A set of multi-primary projector system that could manipulate the ipRGC stimulation were used, while the color and luminance of the background lights were kept constant throughout. Results showed that increased stimulation of ipRGCs under metameric background expanded subjective time. These results suggest that ipRGC stimulation increases arousal/attention so as to expand subjective duration.
In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles
Authors: McVeigh Paul , Cwiklinski Krystyna , Garcia-Campos Andres , Mulcahy Grace , O’Neill Sandra M. , Maule Aaron G. , Dalton John P. 
Published: July 2018

AbstractGlycoproteins secreted by helminth parasites are immunogenic and represent appealing components of vaccine preparations. Our poor knowledge of the pathways that mediate protein glycosylation in parasitic flatworms hinders our understanding of how proteins are synthesised and modified, and our ability to target these pathways for parasite control. Here we provide the first detailed description of genes associated with protein glycosylation in a parasitic flatworm, focusing on the genome of the liver fluke (Fasciola hepatica), which is a globally important trematode parasite of humans and their livestock. Using 190 human sequences as search queries against currently availableF.hepaticagenomes, we identified 149 orthologues with putative roles in sugar uptake or nucleotide sugar synthesis, and an array of glycosyltransferase and glycosidase activities required for protein N- and O-glycosylation. We found appreciable duplication within these orthologues, describing just 87 non-redundant genes when paralogues were excluded.F.hepaticalacks many of the enzymes required to produce complex N- and O-linked glycans, which explains the genomic basis for the structurally simple glycans described byF.hepaticaglycomic datasets, and predicts pervasive structural simplicity in the wider glycome. These data provide a foundation for functional genomic interrogation of these pathways with the view towards novel parasite intervention strategies.
Establishing normal metabolism and differentiation in hepatocellular carcinoma cells by culturing in adult human serum
Authors: Steenbergen Rineke , Oti Martin , ter Horst Rob , Tat Wilson , Neufeldt Chris , Belovodskiy Alexandr , Chua Tiing Tiing , Cho Woo Jung , Joyce Michael , Dutilh Bas E. , Tyrrell D. Lorne 
Published: July 2018

AbstractTissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human hepatoma cells in their native, adult serum (human serum, HS) results in the restoration of key morphological and metabolic features of normal liver cells. When moved to HS, these cells show differential transcription of 22–32% of the genes, stop proliferating, and assume a hepatocyte-like morphology. Metabolic analysis shows that the Warburg-like metabolic profile, typical for FBS-cultured cells, is replaced by a diverse metabolic profile consistent within vivohepatocytes, including the formation of large lipid and glycogen stores, increased glycogenesis, increased beta-oxidation and ketogenesis, and decreased glycolysis. Finally, organ-specific functions are restored, including xenobiotics degradation and secretion of bile, VLDL and albumin. Thus, organ-specific functions are not necessarily lost in cell cultures, but might be merely suppressed in FBS. The effect of serum is often overseen in cell culture and we provide a detailed study in the changes that occur and provide insight in some of the serum components that may play a role in the establishment of the differentiated phenotype.
Possible deep connection between volcanic systems evidenced by sequential assimilation of geodetic data
Authors: Bato Mary Grace , Pinel Virginie , Yan Yajing , Jouanne François , Vandemeulebrouck Jean 
Published: July 2018

AbstractThe existence of possible deep connections between nearby volcanoes has so far only been formulated on the basis of correlation in their eruptive activities or geochemical arguments. The use of geodetic data to monitor the deep dynamics of magmatic systems and the possible interference between them has remained limited due to the lack of techniques to follow transient processes. Here, for the first time, we use sequential data assimilation technique (Ensemble Kalman Filter) on ground displacement data to evaluate a possible interplay between the activities of Grímsvötn and Bárðarbunga volcanoes in Iceland. Using a two-reservoir dynamical model for the Grímsvötn plumbing system and assuming a fixed geometry and constant magma properties, we retrieve the temporal evolution of the basal magma inflow beneath Grímsvötn that drops by up to 85% during the 10 months preceding the initiation of the Bárðarbunga rifting event. We interpret the loss of at least 0.016 km3 in the magma supply of Grímsvötn as a consequence of magma accumulation beneath Bárðarbunga and subsequent feeding of the Holuhraun eruption 41 km away. We demonstrate that, in addition to its interest for predicting volcanic eruptions, sequential assimilation of geodetic data has a unique potential to give insights into volcanic system roots.