bluuebunny/crossref_metadata_2025_split huggingface dataset:
A mechanistic evaluation of the angiogenic properties of a dehydrated amnion chorion membrane in vitro and in vivo
Authors: McQuilling John P. , Burnette Miranda , Kimmerling Kelly A. , Kammer MaryRose , Mowry Katie C. 
Published: August 2019

AbstractAngiogenesis is essential for the successful repair of tissues; however, in many chronic conditions, angiogenesis is inhibited. Placental tissues have been shown to illicit an angiogenic response both in vitro and in vivo, and the angiogenic properties of these tissues likely contribute to observed clinical outcomes. Although there is some work describing the angiogenic effects of these tissues, comparatively little has been done to determine the possible mechanisms responsible for this effect. The purpose of this study was to conduct a thorough evaluation of a commercially available dehydrated amnion chorion membrane to better understand how these tissues may promote angiogenesis. The proteomic content of this tissue was evaluated using a high throughput proteomic microarray, and then the effects of these grafts were evaluated in vivo using subcutaneous gelfoam sponge implants containing conditioned media (CM) from the graft. Human microvascular endothelial cells were then used to determine how released factors effect migration, proliferation, gene expression, and protein production in vitro. Finally, to elucidate potential signaling‐pathways through which tissue‐derived factors act to induce pro‐angiogenetic phenotypes in endothelial cells in vitro, we performed a global analysis of both serine/threonine and tyrosine kinase activity. Kinomic and proteomic data were then combined to generate protein–protein interaction networks that enabled the identification of multiple growth factors and cytokines with both pro‐ and anti‐angiogenetic properties. In vivo, the addition of CM resulted in increased CD31 and αSMA staining and increases in pro‐angiogenic gene expression. In vitro, CM resulted in significant increases in endothelial proliferation, migration, and the expression of granulocyte‐macrophage colony‐stimulating factor, hepatocyte growth factor, and transforming growth factor beta‐3. Integrated kinomic analysis implicated ERK1/2 signaling as the primary pathway activated following culture of endothelial cells with dehydrated amnion/chorion membrane (dACM) CM. In conclusion, dACM grafts triggered pro‐angiogenic responses both in vitro and in vivo that are likely at least partially mediated by ERK1/2 signaling.
DNA Splicing Systems with at Most Two Cutting Sites of a Non-Palindromic Restriction Enzyme
Authors: Ismail Nurul Izzaty , Heng Fong Wan , Sarmin Nor Haniza 
Published: August 2019

The modelling of splicing systems is simulated by the process of cleaving and recombining DNA molecules with the presence of a ligase and restriction enzymes which are biologically called as endodeoxyribonucleases. The molecules resulting from DNA splicing systems are known as splicing languages. Palindrome is a sequence of strings that reads the same forward and backward. Research on generalisations of splicing languages in DNA splicing system involving palindromic sequences for restriction enzymes has been done previously. In this research, the splicing languages resulting from DNA splicing systems with one non-palindromic restriction enzyme are determined using the notation from Head splicing system. The generalisations of splicing languages for DNA splicing systems involving one and two non-overlapping cutting sites of one non-palindromic restriction enzyme are presented in the first and second theorems, respectively, which are proved using direct and induction methods. The result from the first theorem shows a trivial string which is the initial DNA molecule; while the second theorem determines a splicing language consisting of a set of resulting DNA molecules from the respective DNA splicing system.
Postoperative handover among nurses in an orthopedic surgical setting in Myanmar: a best practice implementation project
Authors: Tun Khin Sanda , Wai Khin San , Yin Yin , Thein May Khin 
Published: August 2019

ABSTRACT

Objectives:
The aim of this project was to improve the postoperative handover practice within the local context of an orthopedic surgical setting by implementing best practice.

Introduction:
Clinical handover is a communicative process where the responsibility of patient care is transferred through the exchange of patient information between the care providers. Postoperative handover is an important phase of perioperative care that presents challenges to handover personnel due to transitions in care throughout the perioperative period and the inability of surgical patients to participate in their own care. This paper reports on the best practice implementation project conducted in the field of postoperative handover among nurses in a 500-bed orthopedic surgical setting in Myanmar in 2017.

Methods:
The project used the JBI Practical Application of Clinical Evidence System and the Getting Research into Practice audit tool to conduct a baseline audit and two follow-up audits. A total of 120 postoperative handovers were observed and data were collected. Education sessions and a series of discussion and engagement efforts were employed to increase the compliance with evidence-based postoperative handover practice.

Results:
Baseline audit showed low compliance in audit criteria 3, 5 and 6, whereas varying compliance was observed in criteria 1, 2 and 4. Improvement was seen with five criteria in follow-up audits except for criterion 1. Compliance with criterion 1 was inconclusive because staff attendance at postoperative handovers varied, depending on patients’ different handover needs. Nurses attendance, however, improved through engagement efforts and education sessions.

Conclusion:
We were able to make significant improvements in the underperforming areas related to postoperative patient handover. This project confirms that an SBAR (Situation, Background, Assessment, Recommendation) checklist has been implemented to navigate and document every postoperative handover at the main operating theater; the handover process at the intensive care unit complies with the COLD (Connect, Observe, Listen, Delegate) process; and attendance of handovers by nurses has increased. It is recommended that regular audits be conducted to sustain the change and improve where required.
Contribution of adenosine and ATP to the carotid body chemosensory activity in ageing
Authors: Sacramento Joana F. , Olea Elena , Ribeiro Maria J. , Prieto‐Lloret Jesus , Melo Bernardete F. , Gonzalez Constancio , Martins Fatima O. , Monteiro Emilia C. , Conde Silvia V. 
Published: August 2019

Key points
Adenosine and ATP are excitatory neurotransmitters involved in the carotid body (CB) response to hypoxia.
During ageing the CB exhibits a decline in its functionality, demonstrated by decreased hypoxic responses.
In aged rats (20–24 months old) there is a decrease in: basal and hypoxic release of adenosine and ATP from the CB; expression of adenosine and ATP receptors in the petrosal ganglion; carotid sinus nerve (CSN) activity in response to hypoxia; and ventilatory responses to ischaemic hypoxia. There is also an increase in SNAP25, ENT1 and CD73 expression.
It is concluded that, although CSN activity and ventilatory responses to hypoxia decrease with age, adjustments in purinergic metabolism in the CB in aged animals are present aiming to maintain the contribution of adenosine and ATP.
The possible significance of the findings in the context of ageing and in CB‐associated pathologies is considered.
AbstractDuring ageing the carotid body (CB) exhibits a decline in its functionality. Here we investigated the effect of ageing on functional CB characteristics as well as the contribution of adenosine and ATP to CB chemosensory activity. Experiments were performed in 3‐month‐old and 20‐ to 24‐month‐old male Wistar rats. Ageing decreased: the number of tyrosine hydroxylase immune‐positive cells, but not type II cells or nestin‐positive cells in the CB; the expression of P2X2 and A2A receptors in the petrosal ganglion; and the basal and hypoxic release of adenosine and ATP from the CB. Ageing increased ecto‐nucleotidase (CD73) immune‐positive cells and the expression of synaptosome associated protein 25 (SNAP25) and equilibrative nucleoside transporter 1 (ENT1) in the CB. Additionally, ageing did not modify basal carotid sinus nerve (CSN) activity or the activity in response to hypercapnia, but decreased CSN activity in hypoxia. The contribution of adenosine and ATP to stimuli‐evoked CSN chemosensory activity in aged animals followed the same pattern of 3‐month‐old animals. Bilateral common carotid occlusions during 5, 10 and 15 s increased ventilation proportionally to the duration of ischaemia, an effect decreased by ageing. ATP contributed around 50% to ischaemic‐ventilatory responses in young and aged rats; the contribution of adenosine was dependent on the intensity of ischaemia, being maximal in ischaemias of 5 s (50%) and much smaller in 15 s ischaemias. Our results demonstrate that both ATP and adenosine contribute to CB chemosensory activity in ageing. Though CB responses to hypoxia, but not to hypercapnia, decrease with age, the relative contribution of both ATP and adenosine for CB activity is maintained.
Vitamin D status among adults (18–65 years old) attending primary healthcare centres in Qatar: a cross-sectional analysis of the Electronic Medical Records for the year 2017
Authors: Zainel Abdul-Jaleel A Latif , Qotba Hamda , Al Nuaimi Ahmed , Syed Mohamed 
Published: August 2019

ObjectivesTo investigate the prevalence of vitamin D deficiency among individuals attending primary healthcare facilities in Qatar and to assess the association between vitamin D deficiency and some medical conditions in persons aged 18–65 years old.SettingThe study was undertaken in publicly funded primary healthcare services in the State of Qatar.ParticipantsA total of 102 342 participants aged between 18 and 65 years old with a valid serum vitamin D test result during the year 2017.Outcome measuresSerum level <10 ng/mL (<25 nmol/L) was defined as severe vitamin D deficiency, a serum level of <20 ng/mL (<50 nmol/L) was defined as vitamin D deficiency and a serum level <30 ng/mL (<75 nmol/L) defined as vitamin D insufficiency.ResultsThe prevalence rate of severe vitamin D deficiency was 14.1% among study participants with no history of vitamin D replacement therapy in the previous months. The prevalence rate of vitamin D deficiency was as high as 71.4% and that of vitamin D insufficiency was up to 92.7%. None of the five chronic conditions explored in the study (diabetes, hypertension, asthma, stroke and cardiovascular disease) had an obvious association with severe vitamin D deficiency status in a bivariate analysis. However, multivariate modelling showed that (adjusting for age, gender, body mass index and nationality and each of the included chronic conditions) hypertension, cardiovascular diseases and stroke placed an individual at a higher risk of having an associated severe vitamin D deficiency status.ConclusionAlthough not comprehensive and nationally representative, this study is suggestive of a higher prevalence of vitamin D deficiency among young adults, females, Qatari nationality and those with higher body mass index. Multivariate modelling showed that hypertension, cardiovascular diseases and stroke were associated with a higher risk of severe vitamin D deficiency status.
Protocol for a feasibility randomised trial of low-intensity interventions for antenatal depression: ADAGIO trial comparing interpersonal counselling with cognitive behavioural therapy
Authors: Ingram Jenny , Johnson Debbie , Johnson Sarah , O'Mahen Heather A , Kessler David , Taylor Hazel , Law Roslyn , Round Jeff , Ford Jenny , Hopley Rebecca , Glynn Joel , Culpin Iryna , Evans Jonathan 
Published: August 2019

IntroductionOne in eight women suffer from depression during pregnancy. Currently, low-intensity brief treatment based on cognitive behavioural therapy (CBT) is the only talking treatment widely available in the National Health Service (NHS) for mild and moderate depression. CBT involves identifying and changing unhelpful negative thoughts and behaviours to improve mood. Mothers in our patient advisory groups requested greater treatment choice. Interpersonal counselling (IPC) is a low-intensity version of interpersonal therapy. It may have important advantages during pregnancy over CBT because it targets relationship problems, changes in role and previous losses (eg, miscarriage). We aim to compare CBT and IPC for pregnant women with depression in a feasibility study.Methods and analysisA two-arm non-blinded randomised feasibility study of 60 women will be conducted in two UK localities. Women with depression will be identified through midwife clinics and ultrasound scanning appointments and randomised to receive six sessions of IPC or CBT. In every other way, these women will receive usual care. Women thought to have severe depression will be referred for more intensive treatment. After 12 weeks, we will measure women’s mood, well-being, relationship satisfaction and use of healthcare. Women, their partners and staff providing treatments will be interviewed to understand whether IPC is an acceptable approach and whether changes should be introduced before applying to run a larger trial.Several groups of patients with depression during pregnancy have contributed to our study design. A patient advisory group will meet and advise us during the study.Ethics and disseminationStudy results will inform the design of a larger multicentre randomised controlled trial (RCT). Our findings will be shared through public engagement events, papers and reports to organisations within the NHS. National Research Ethics Service Committee approved the study protocol.Trial registration numberISRCTN11513120.
Viruses in Skin Cancer (VIRUSCAN): Study Design and Baseline Characteristics of a Prospective Clinic-Based Cohort Study
Authors: Amorrortu Rossybelle P. , Fenske Neil A. , Cherpelis Basil S. , Vijayan Laxmi , Zhao Yayi , Balliu Juliana , Messina Jane L. , Sondak Vernon K. , Giuliano Anna R. , Waterboer Tim , Pawlita Michael , Gheit Tarik , Tommasino Massimo , Rollison Dana E. 
Published: August 2019

Abstract

Background:
Accumulating evidence suggests that cutaneous viral infections are risk factors for the development of keratinocyte carcinomas. The Viruses in Skin Cancer (VIRUSCAN) Study, a prospective cohort study, was established in 2014 to investigate the risk of keratinocyte carcinoma associated with cutaneous human papillomavirus and polyomavirus infection and the possible interaction with ultraviolet radiation exposure (UVR).

Methods/Results:
VIRUSCAN incorporates repeated measures of viral infection using multiple markers of infection and quantitative measures of UVR using a spectrophotometer. Participants were recruited between July 14, 2014 and August 31, 2017 at the University of South Florida Dermatology Clinic in Tampa, FL. After excluding 124 individuals with prevalent keratinocyte carcinomas at baseline, 1,179 participants (53.2% women, 46.8% men, all ages 60 years and older) were followed for up to 4 years with routine skin exams occurring every 6 to 12 months. Here, we present the VIRUSCAN Study design, methods, and baseline characteristics, including demographics, sun exposure behavior, quantitative UVR exposure measurements, and cutaneous viral prevalence, for the full study cohort.

Conclusions:
The VIRUSCAN Study will provide critical temporal evidence needed to assess the causality of the role cutaneous viral infections play in the development of keratinocyte carcinomas, as well as the potential interaction between cutaneous viral infections and UVR exposure.

Impact:
Study findings will be valuable in future development of novel keratinocyte carcinoma prevention strategies.
Prediagnostic Leukocyte Telomere Length and Pancreatic Cancer Survival
Authors: Hamada Tsuyoshi , Yuan Chen , Bao Ying , Zhang Mingfeng , Khalaf Natalia , Babic Ana , Morales-Oyarvide Vicente , Cochrane Barbara B. , Gaziano J. Michael , Giovannucci Edward L. , Kraft Peter , Manson JoAnn E. , Ng Kimmie , Nowak Jonathan A. , Rohan Thomas E. , Sesso Howard D. , Stampfer Meir J. , Amundadottir Laufey T. , Fuchs Charles S. , De Vivo Immaculata , Ogino Shuji , Wolpin Brian M. 
Published: August 2019

Abstract

Background:
Leukocyte telomere length has been associated with risk of subsequent pancreatic cancer. Few prospective studies have evaluated the association of prediagnostic leukocyte telomere length with pancreatic cancer survival.

Methods:
We prospectively examined the association of prediagnostic leukocyte telomere length with overall survival (OS) time among 423 participants diagnosed with pancreatic adenocarcinoma between 1984 and 2008 within the Health Professionals Follow-up Study, Nurses' Health Study, Physicians' Health Study, and Women's Health Initiative. We measured prediagnostic leukocyte telomere length in banked blood samples using quantitative PCR. Cox proportional hazards models were used to estimate HRs for OS with adjustment for potential confounders. We also evaluated 10 SNPs at the telomerase reverse transcriptase locus.

Results:
Shorter prediagnostic leukocyte telomere length was associated with reduced OS among patients with pancreatic cancer (Ptrend = 0.04). The multivariable-adjusted HR for OS comparing the lowest with highest quintiles of leukocyte telomere length was 1.39 (95% confidence interval, 1.01–1.93), corresponding to a 3-month difference in median OS time. In an analysis excluding cases with blood collected within 2 years of cancer diagnosis, the association was moderately stronger (HR, 1.55; 95% confidence interval, 1.09–2.21; comparing the lowest with highest quintiles; Ptrend = 0.01). No prognostic association or effect modification for the prognostic association of prediagnostic leukocyte telomere length was noted in relation to the studied SNPs.

Conclusions:
Prediagnostic leukocyte telomere length was associated with pancreatic cancer survival.

Impact:
Prediagnostic leukocyte telomere length can be a prognostic biomarker in pancreatic cancer.
A Hypoxia-Inducible HIF1–GAL3ST1-Sulfatide Axis Enhances ccRCC Immune Evasion via Increased Tumor Cell–Platelet Binding
Authors: Robinson Claire M. , Poon Betty P.K. , Kano Yoshihito , Pluthero Fred G. , Kahr Walter H.A. , Ohh Michael 
Published: August 2019

Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common form of kidney cancer and the major cause of mortality for individuals with von Hippel-Lindau (VHL) disease. ccRCC is characterized most frequently by inactivation of VHL tumor suppressor protein that mediates degradation of the alpha subunit of the hypoxia-inducible factor (HIF) transcription factor family. HIF has been implicated in disease progression and the aim of this study was to identify novel HIF target genes that may contribute to ccRCC. We show that GAL3ST1, an enzyme that catalyzes the sulfonation of the plasma membrane sulfolipid sulfatide, is among the top 50 upregulated genes in ccRCC tissue relative to matched normal tissue. Increased expression of GAL3ST1 in primary ccRCC correlates with decreased survival. We show that GAL3ST1 is a HIF target gene whose expression is induced upon VHL loss leading to the accumulation of its enzymatic product sulfatide. Notably, platelets bind more efficiently to renal cancer cells with high GAL3ST1-sulfatide expression than to GAL3ST1-sulfatide–negative counterparts, which protects ccRCC cells against natural killer cell–mediated cytotoxicity. These results suggest that GAL3ST1 is a HIF-responsive gene that may contribute to ccRCC development via promoting cancer cell evasion of immune surveillance.

Implications:
Cancer development is in part dependent on evasion of immune response. We identify a HIF target gene product GAL3ST1 that may play a role in this critical process.
Associations of Self‐Reported History of Depression and Antidepressant Use Before Stroke Onset With Poststroke Post–Acute Rehabilitation Care—An Exploratory Study: The BASIC (Brain Attack Surveillance in Corpus Christi) Project
Authors: Stulberg Eric L. , Dong Liming , Zheutlin Alexander R. , Kim Sehee , Claflin Edward S. , Skolarus Lesli E. , Morgenstern Lewis B. , Lisabeth Lynda D. 
Published: August 2019

Background

Prestroke depression status and post–acute rehabilitation care (
PARC
) are determinants of poststroke depression and function. However, little is known on how prestroke depression status affects
PARC
placement, a possible pathway for upstream intervention. We examined how prestroke depression status affects
PARC
in a population‐based study.

Methods and Results

Incident ischemic stroke cases were from the BASIC (Brain Attack Surveillance in Corpus Christi) Project from 2008 to 2012. Prestroke depression status was self‐reported and categorized as (1) never depressed, (2) history of depression without antidepressant use before stroke onset, or (3) antidepressant use before stroke onset.
PARC
included home, a skilled nursing facility, or an inpatient rehabilitation facility. Confounder‐adjusted multinomial regression models were used to examine the association between prestroke depression status and
PARC
. Adjustment for stroke severity was deferred in the main analyses because it may lie on the causal pathway. There were 548 stroke survivors (mean age 65.3 years, 48.3% female, 62.6% Mexican‐American). The adjusted odds ratios comparing home discharge to a skilled nursing facility were 1.88 (95%
CI
: 0.86‐4.11) for those with a history of depression and 2.55 (95%
CI
: 1.11‐5.83) for those using an antidepressant at stroke onset, relative to those never depressed. The adjusted odds ratios comparing an inpatient rehabilitation facility to a skilled nursing facility were 1.17 (95%
CI
0.40‐3.42) and 3.28 (95%
CI
1.24‐8.67) for those with a history of depression and those using an antidepressant at stroke onset, respectively, relative to those never depressed.

Conclusions
Antidepressant use before stroke onset may increase odds of home and inpatient rehabilitation facility discharge compared with skilled nursing facility discharge.
Evaluation of agomelatine for the treatment of sleep problems in adults with autism spectrum disorder and co-morbid intellectual disability
Authors: Ballester Pura , Martínez María José , Inda María-del-Mar , Javaloyes Auxiliadora , Richdale Amanda L , Muriel Javier , Belda César , Toral Natalia , Morales Domingo , Fernández Eduardo , Peiró Ana M 
Published: August 2019

Purpose: Intellectual disability (ID) and autism spectrum disorder (ASD) are common, co-occurring developmental disorders and are frequently associated with sleep problems. This study aimed to assess the effectiveness and tolerability of agomelatine as a pharmacotherapy for sleep problems in ASD adults with ID. Method: A randomised, crossover, triple-blind, placebo-controlled clinical trial, with two three-month periods of treatment starting with either agomelatine or placebo and a washout period of two weeks. Ambulatory circadian monitoring (24 hours/7 days) evaluated total sleep time (TST) as the primary outcome variable. Results: Participants ( N=23; 35±12 years old; 83% male) had a median of three (interquartile range (IQR) 1–4) co-morbidities and were taking a median of five (IQR 2–7) prescribed drugs. Before agomelatine or placebo treatment, all subjects presented with insomnia symptoms, including sleep latency (100% abnormal, 55±23 minutes) or TST (55% abnormal, 449±177 minutes), and 66% had circadian rhythm sleep–wake abnormalities with rhythm phase advancements according to the M5 sleep phase marker values. During the three-month agomelatine treatment, night TST significantly increased by a mean of 83 minutes (16% abnormal, 532±121 minutes), together with a phase correction (M5 1:45±2:28 hours vs. 3:15±2:20 hours), improving sleep stability in wrist temperature rhythm (0.43±0.29 vs. 0.52±0.18 AU). Adverse events were mild and transient. Conclusions: Agomelatine was effective and well tolerated for treating insomnia and circadian rhythm sleep problems present in adults with ASD and ID.