bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel protein encoded by a circular RNA circPPP1R12A promotes tumor pathogenesis and metastasis of colon cancer via Hippo-YAP signaling
Authors: Zheng Xiao , Chen Lujun , Zhou You , Wang Qi , Zheng Zhuojun , Xu Bin , Wu Chen , Zhou Qi , Hu Wenwei , Wu Changping , Jiang Jingting 
Published: March 2019

Abstract
Background
It has been well established that circular RNAs (circRNAs) play an important regulatory role during tumor progression. Recent studies have indicated that even though circRNAs generally regulate gene expression through miRNA sponges, they may encode small peptides in tumor pathogenesis. However, it remains largely unexplored whether circRNAs are involved in the tumorigenesis of colon cancer (CC).

Methods
The expression profiles of circRNAs in CC tissues were assessed by circRNA microarray. Quantitative real-time PCR, RNase R digestion assay and tissue microarray were used to confirm the existence and expression pattern of circPPP1R12A. The subcellular distribution of circPPP1R12A was analyzed by nuclear mass separation assay and fluorescence in situ hybridization (FISH). SDS-PAGE and LC/MS were employed to evaluate the protein-coding ability of circPPP1R12A. CC cells were stably transfected with lentivirus approach, and cell proliferation, migration and invasion, as well as tumorigenesis and metastasis in nude mice were assessed to clarify the functional roles of circPPP1R12A and its encoded protein circPPP1R12A-73aa. RNA-sequencing and Western blotting analysis were furthered employed to identify the critical signaling pathway regulated by circPPP1R12A-73aa.

Results
We firstly screened the expression profiles of human circRNAs in CC tissues and found that the expression of hsa_circ_0000423 (termed as circPPP1R12A) was significantly increased in CC tissues. We also found that circPPP1R12A was mostly localized in the cytoplasm of CC cells. Kaplan–Meier analysis showed that patients with higher levels of circPPP1R12A had a significantly shorter overall survival. By gain- and loss-of-function approaches, the results suggested that circPPP1R12A played a critical role in proliferation, migration and invasion of CC cells. Furthermore, we showed that circPPP1R12A carried an open reading frame (ORF), which encoded a functional protein (termed as circPPP1R12A-73aa). Next, we found that PPP1R12A-C, not circPPP1R12A, promoted the proliferation, migration and invasion abilities of CC in vitro and in vivo. Finally, we identified that circPPP1R12A-73aa promoted the growth and metastasis of CC via activating Hippo-YAP signaling pathway. In addition, the YAP specific inhibitor Peptide 17 dramatically alleviated the promotive effect of circPPP1R12A-73aa on CC cells.

Conclusions
In the present study, we illustrated the coding-potential of circRNA circPPP1R12A in the progression of CC. Moreover, we identified that circPPP1R12A-73aa promoted the tumor pathogenesis and metastasis of CC via activating Hippo-YAP signaling pathway. Our findings might provide valuable insights into the development of novel potential therapeutic targets for CC.
Seasonal variation in antidepressant prescriptions, environmental light and web queries for seasonal affective disorder
Authors: Lansdall-Welfare Thomas , Lightman Stafford , Cristianini Nello 
Published: March 2019

SummaryThe state of an individual's mental health depends on many factors. Determination of the importance of any particular factor within a population needs access to unbiased data. We used publicly available data-sets to investigate, at a population level, how surrogates of mental health covary with light exposure. We found strong seasonal patterns of antidepressant prescriptions, which show stronger correlations with day length than levels of solar energy. Levels of depression in a population can therefore be determined by proxy indicators such as web query logs. Furthermore, these proxies for depression correlate with day length rather than solar energy.Declaration of interestNone.
A Simple Method for Estimating Hand Hygiene Use Among Anesthesia Personnel: Development, Validation, and Use in a Quality Improvement Project
Authors: Segal Scott , Harris Hannah M. , Gunawan Antonius , Schumann Roman 
Published: March 2019

BACKGROUND:
Frequent hand hygiene by anesthesia personnel may be an important factor in reducing contamination of IV lines and medication access ports and may reduce hospital-acquired infections. Measurement of hand hygiene frequency at the individual clinician level by direct observation or electronic devices is cumbersome and expensive. We developed and validated a simple method for estimating hand hygiene frequency by individual anesthesia providers and utilized it in a quality improvement initiative to increase hand hygiene use.

METHODS:
Pump-style, alcohol-based hand hygiene container weight at the anesthesia work station was measured before and after each surgical operation and converted to estimated number of accesses (pumps) per hour. Video observation was used to validate the estimated hand hygiene use. A quality improvement initiative utilized periodic measurement of hand hygiene frequency via the validated method, and incorporated individual provider feedback, email reminders, monthly departmental performance reports, and reminders in the electronic anesthesia record. Segmented linear regression was used to evaluate the effect of the intervention on hand hygiene use.

RESULTS:
Delivered product per pump was consistent for containers at least half-full and averaged (mean ± SD) 0.92 ± 0.13 g per pump. Video observation in 26 cases showed a strong correlation between observed hand hygiene episodes and estimated hand hygiene use frequency based on weight change of the container (linear regression, R
2 = 0.97, P < .0001). Median hand hygiene frequency was near 0 at baseline but increased progressively throughout the intervention period (segmented linear regression, overall R
2 = 0.76, P < .0001; change of intercept or mean hand hygiene after initiation of intervention [parameter estimate ± SE] [0.970 ± 0.29], P = .0008).

CONCLUSIONS:
A low-cost, simple method for measuring individual anesthesia clinician use of hand hygiene intraoperatively based on container weight change is feasible and sufficiently accurate to support a quality improvement initiative to increase its use.
Do You Really Mean It? Assessing the Strength, Frequency, and Reliability of Applicant Commitment Statements During the Anesthesiology Residency Match
Authors: Moran Kenneth R. , Schell Randall M. , Smith Kathleen A. , Abdel-Rasoul Mahmoud , Lekowski Robert W. , Rankin Demicha D. , DiLorenzo Amy , McEvoy Matthew D. 
Published: March 2019

BACKGROUND:
Despite the critical nature of the residency interview process, few metrics have been shown to adequately predict applicant success in matching to a given program. While evaluating and ranking potential candidates, bias can occur when applicants make commitment statements to a program. Survey data show that pressure to demonstrate commitment leads applicants to express commitment to multiple institutions including telling >1 program that they will rank them #1. The primary purpose of this cross-sectional observational study is to evaluate the frequency of commitment statements from applicants to 5 anesthesiology departments during a single interview season, report how often each statement is associated with a successful match, and identify how frequently candidates incorrectly represented commitments to rank a program #1.

METHODS:
During the 2014 interview season, 5 participating anesthesiology programs collected written and verbal communications from applicants. Three residency program directors independently reviewed the statements to classify them into 1 of 3 categories; guaranteed commitment, high rank commitment, or strong interest. Each institution provided a deidentified rank list with associated commitment statements, biographical data, whether candidates were ranked-to-match, and if they successfully matched.

RESULTS:
Program directors consistently differentiated among strong interest, high rank, and guaranteed commitment statements with κ coefficients of 0.9 (95% CI, 0.8–0.9) or greater between any pair of reviewers. Overall, 35.8% of applicants (226/632) provided a statement demonstrating at least strong interest and 5.4% (34/632) gave guaranteed commitment statements. Guaranteed commitment statements resulted in a 95.7% match rate to that program in comparison to statements of high rank (25.6%), strong interest (14.6%), and those who provided no statement (5.9%). For those providing guaranteed commitment statements, it can be assumed that the 1 candidate (4.3%) who did not match incorrectly represented himself. Variables such as couples match, “R” positions, and not being ranked-to-match on both advanced and categorical rank lists were eliminated because they can result in a nonmatch despite truthfully ranking a program #1.

CONCLUSIONS:
Each level of commitment statement resulted in a progressively increased frequency of a successful match to the recipient program. Only 5.4% of applicants committed to rank a program #1, but these statements were very reliable. These data can help program directors interpret commitment statements and assist accurate evaluation of the interest of candidates throughout the match process.
Cortical mitochondria regulate insulin secretion by local Ca2+ buffering in rodent beta cells
Authors: Griesche Nadine , Sanchez Gonzalo , Hermans Cedric , Idevall-Hagren Olof 
Published: March 2019

ABSTRACT
Mitochondria play an essential role in regulating insulin secretion from beta cells by providing the ATP needed for the membrane depolarization that results in voltage-dependent Ca2+ influx and subsequent insulin granule exocytosis. Ca2+, in turn, is also rapidly taken up by the mitochondria and exerts important feedback regulation of metabolism. The aim of this study was to determine whether the distribution of mitochondria within beta cells is important for the secretory capacity of these cells. We find that cortically localized mitochondria are abundant in rodent beta cells, and that these mitochondria redistribute towards the cell interior following depolarization. The redistribution requires Ca2+-induced remodeling of the cortical F-actin network. Using light-regulated motor proteins, we increased the cortical density of mitochondria twofold and found that this blunted the voltage-dependent increase in cytosolic Ca2+ concentration and suppressed insulin secretion. The activity-dependent changes in mitochondria distribution are likely to be important for the generation of Ca2+ microdomains required for efficient insulin granule release.
Economic valuation from direct use of mangrove forest restoration in Balikpapan Bay, East Kalimantan, Indonesia
Authors: Lahjie Abubakar M. , Nouval Bagus , Lahjie Annisa Abubakar , Ruslim Yosep , Kristiningrum Rochadi 
Published: March 2019

Background: The mangrove forests in Balikpapan Bay, Indonesia, have been used as a source of livelihood for local community more than 150 years. Since the natural products of the mangrove forest, such as wood and seafood, are not able to meet the economic needs of the local community, some areas have been converted into brackish water ponds with traditional aquaculture systems. The growth of brackish water ponds over the last five decades has been identified as the main cause of ecosystem destruction. However, the mangrove ecosystem has been restored naturally through tidal action and seeds falling from mangrove trees. Methods: This study focused on the mangrove tree species Rhizophora apiculata, with ages ranging from 3 to 40 years. Initially, the study site (area, 1 ha) was plotted. The study sample size included 30% of the local population, chosen by systematic random sampling. The data collection was undertaken as follows: 1) measurement of the diameter and height of mangrove trees; 2) observation of local fish auctions; and 3) interviewing of fishers and local communities regarding the direct use of the natural products of the mangrove ecosystem. Results: It is suggested that the total income from wood production is IDR 742,425,000 year-1 or US $0.933 person-1 day-1. Furthermore, the total income from fishing is IDR 1,080,353,280 year-1 or US $1.43 person-1 day-1. Pre-thinning income level for wood harvesting is still low. The income difference between wood production and fishing resulted in the rate of overfishing reaching 45.5%. The highest observed wood production was reached at the age of 25 years, and the highest value of mean annual increment (MAI) is 5.39 m3 ha-1 at the age of 40 years. Conclusions: This study showed that tree thinning, ranging from 90 to 350 trees ha-1, can increase the value of MAI by around 24.5%.
A narrative synthesis scoping review of life course domains within health service utilisation frameworks
Authors: O'Donovan Mary-Ann , McCallion Phillip , McCarron Mary , Lynch Louise , Mannan Hasheem , Byrne Elaine 
Published: March 2019

Background: Current thinking in health recognises the influence of early life experiences (health and otherwise) on later life outcomes. The life course approach has been embedded in the work of the World Health Organisation since the Ageing and Health programme was established in 1995. Yet there has been limited debate on the relevancy of a life course lens to understanding health service utilisation. Aim: The aim of the review was twofold. Firstly, identify existing healthcare utilisation frameworks other than the dominant Andersen’s behavioural model currently in use. Secondly, to identify if current frameworks incorporate the advocated life course perspective in understanding health service utilisation. Methods: A scoping review of PubMed, Cinahl Plus, Emerald, PsycINFO, Web of Knowledge and Scopus was conducted. Data extraction used a framework approach with meta-synthesis guided by the four domains of the life course proposed by Elder (1979): human agency, location, temporality and relationships, and interdependencies. Results: A total of 551 papers were identified, with 70 unique frameworks (other than Andersen’s Behavioural Model) meeting the inclusion criteria and included in the review. Conclusion: To date there has been limited explicit discussion of health service utilisation from a life course perspective. The current review highlights a range of frameworks that draw on aspects of the life course, but have been used with this perspective in mind. The life course approach highlights important gaps in understanding and assessing health service utilisation (HSU), such as utilisation over time. HSU is a complex phenomenon and applying a structured framework from a life course perspective would be of benefit to researchers, practitioners and policy makers.
A review of compliance with pain assessments within a UK ICU
Authors: Melia Rachel , Morrell-Scott Nicola , Maine Norman 
Published: March 2019

Background: clinical audits highlight areas where care may not be of the desired quality; they are essential to ensure care is safe and effective. Effective assessment and management of pain have been shown to improve patient wellbeing and clinical outcomes. Aim: this audit aimed to identify compliance with pain assessment tools and documentation within intensive care and make recommendations to improve practice. Discussion: compliance with documenting pain assessments was poor, a finding that is consistent with the literature. Although a wealth of evidence has shown pain assessments are not being completed effectively, this continues to be a problem. Intensive care has significant areas for improvement in this area, which would improve patients' experiences and outcomes. Nurses should be educated in the use of pain assessment tools and compliance. Conclusion: providing patients in intensive care with appropriate analgesia benefits their physical and psychological health. Areas for improvement identified in this audit include that pain assessments need to be carried out and documented regularly. The audit has implications for practice in that it shows a need for reinforced education for staff, better communication and updates to promote pain assessment and the implementation of guidelines.
Witnessing Horrorism: The Piteşti Experiment
Authors: Ionescu Arleen 
Published: March 2019

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This article presents an irrational, sadistic experiment based on cruelty and complete disregard of human values, carried out between 1949 and 1952 in Romania and known under the infamous name of the Piteşti experiment. Its agenda was based on ‘re-education’, metaphorically presented as a sort of ‘healing’, supposedly performed to eliminate the ‘rot’ from prisoners. In the light of existent theories on the process of witnessing and testifying, I explore Dumitru Bacu’s, Grigore Dumitrescu’s, Paul Goma’s and Virgil Ierunca’s accounts of this despicable experiment and discuss the different roles assumed by witnesses while writing about Piteşti. Using the notions of ‘terror’ and ‘horror’ as developed in the works of Hannah Arendt and Adriana Cavarero, as well as Judith Lewis Herman’s findings from Trauma and Recovery and Anne-Marie Roviello’s work on ‘the hidden violence of totalitarianism’, the article suggests how the Piteşti experiment can be compared to the Shoah. Through this comparison, I point out the ‘unnarratability’ of the events that happened in the Piteşti penitentiary and, explaining the positions of the victims, I discuss the elements that made this experiment a uniquely tragic event in the history of communist prisons: on the one hand, that any tortured prisoner was forced to become the torturer of his fellows, on the other hand, that no one was allowed to die.
Operational Identities for Circular and Hyperbolic Functions and Their Generalizations
Authors: Cassisa C. , Ricci P. E. , Tavkhelidze I. 
Published: March 2019

Abstract
Starting from the exponential, some classes of analytic functions of the derivative operator are studied, including pseudo-hyperbolic and pseudo-circular functions. Some formulas related to operational calculus are deduced, and the important role played in such a context by Hermite–Kampé de Fériet polynomials is underlined.
CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A2BAdenosine Receptor Signaling
Authors: Yan Angela , Joachims Michelle L. , Thompson Linda F. , Miller Andrew D. , Canoll Peter D. , Bynoe Margaret S. 
Published: March 2019

Glioblastoma (GB) is one of the deadliest brain cancers to afflict humans, and it has a very poor survival rate even with treatment. The extracellular adenosine-generating enzyme CD73 is involved in many cellular functions that can be usurped by tumors, including cell adhesion, proliferation, invasion, and angiogenesis. We set out to determine the role of CD73 in GB pathogenesis. To do this, we established a unique GB mouse model (CD73-FLK) in which we spatially expressed CD73 on endothelial cells in CD73−/−mice. This allowed us to elucidate the mechanism of host CD73 versus GB-expressed CD73 by comparing GB pathogenesis in WT, CD73−/−, and CD73-FLK mice. GB in CD73−/−mice had decreased tumor size, decreased tumor vessel density, and reduced tumor invasiveness compared with GB in WT mice. Interestingly, GBs in CD73-FLK mice were much more invasive and caused complete distortion of the brain morphology. We showed a 20-fold upregulation of A2BAR on GB compared with sham, and its activation induced matrix metalloproteinase-2, which enhanced GB pathogenesis. Inhibition of A2BAR signaling decreased multidrug resistance transporter protein expression, including permeability glycoprotein (P-gp) and multidrug resistance-associated protein 1 (MRP1). Further, we showed that blockade of A2BAR signaling potently increased GB cell death induced by the chemotherapeutic drug temozolomide. Together, these findings suggest that CD73 and A2BAR play a multifaceted role in GB pathogenesis and progression and that targeting the CD73–A2BAR axis can benefit GB patients and inform new approaches for therapy to treat GB patients.SIGNIFICANCE STATEMENTGlioblastoma (GB) is the most devastating primary brain tumor. GB patients' median survival is 16 months even with treatment. It is critical that we develop prophylaxes to advance GB treatment and improve patient survival. CD73-generated adenosine has been implicated in cancer pathogenesis, but its role in GB was not ascertained. Here, we demonstrated that host CD73 plays a prominent role in multiple areas of glioblastoma pathogenesis, including promoting GB growth, its angiogenesis, and its invasiveness. We found a 20-fold increase in A2Badenosine receptor (AR) expression on GB compared with sham, and its inhibition increased GB chemosensitivity to temozolomide. These findings strongly indicate that blockade or inhibition of CD73 and the A2BAR are prime targets for future GB therapy.
Travmatik Dental Yaralanmalar Konusunda Hazırlanan Eğitici Broşürün Ebeveyn Bilgi Düzeyi ve Tutumu Üzerine Etkisinin Değerlendirilmesi
Authors: DELİKAN Ebru , KOÇAK Nazan 
Published: March 2019

Amaç: Mersin ilinde yaşayan ebeveynlerin travmatik diş (dental) yaralanmaları konusunda bilgi düzeyinin belirlenmesi ve bu yaralanmalarda yapılması gereken acil müdahalelerle ilgili kolay ve anlaşılır olarak hazırlanmış bir eğitici broşürün bilgi düzeyine etkisinin değerlendirilmesidir. Gereç ve Yöntemler:Çalışmamıza, Mersin Üniversitesi Diş Hekimliği Fakültesi Ağız Diş ve Çene Radyolojisi Anabilim Dalı’na rutin dental muayene için başvuran 114 ebeveyn dahil edildi. Araştırmacılar tarafından ebeveynlere 20 soruluk bir anket formu dağıtıldı ve cevaplamaları istendi. Anket sonrasında; ebeveynlerin travmatik dental yaralanmalar hakkında hazırlanan eğitici broşürleri incelemeleri ve daha sonrasında aynı anketi ikinci kez cevaplamaları sağlandı. Oneway Anova testi, Student t, Paired Sample t test ve McNemar testi kullanılarak istatistiksel analizler yapıldı. Bulgular:Travmatik dental yaralanmalar konusunda ebeveynlerin bilgi düzeyinde demografik verilerin farklılık oluşturmadığı görüldü. Ebeveynlerin çoğu (%60,6) dental travmalar hakkında eğitim almamıştı ve bu tür travmaların acil müdahale gerektirdiğini (%64,4) düşünmüşlerdi. Ebeveynlerin anketteki sorulara eğitici broşür öncesi ve sonrası doğru cevap verme oranları sırasıyla %38,89±15,17 ve %76±23,48’dir ve bu artış istatistiksel olarak anlamlı bulundu (p&lt;0.05). Diş yaralanmalarına müdahale edebileceklerini belirten ebeveynlerin oranı %39,4 oranındadır ancak bu oran eğitici broşür ile bilgilendirildikten sonra %71,2'ye yükselmiştir. TDY yönetimi sorularının yaklaşık olarak tümünde verilen doğru cevap sayısında istatistiksel olarak anlamlı bir artış görülürken, “Avülse süt dişleri yerine yerleştirilir mi?” sorusuna verilen doğru cevapta anlamlı derecede azalma bulunmuştur. (p&lt;0.05) Sonuçlar:Mersin ilinde yaşayan ebeveynlerin eğitim öncesinde travmatik dental yaralanmalar konusunda bilgi düzeylerinin yeterli olmadığı görülmüştür. Ancak broşür ile yapılan bilgilendirme sonrasında bilgi düzeylerindeki artış oldukça memnuniyet vericidir.Anahtar Kelimeler:Anket, Broşür, Diş yaralanmaları, Ebeveyn