bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adherence to guideline recommendations for asthma care in community pharmacies: actual and needed performance
Authors: Kuipers Esther , Wensing Michel , Wong-Go Elaine , Daemen Bernard J. G. , De Smet Peter A. G. M. , Teichert Martina 
Published: July 2019

AbstractPharmaceutical care guidelines aim to provide recommendations for pharmaceutical care, reduce unwanted pharmacy practice variation and ultimately improve the quality of healthcare. This study evaluated community pharmacists’ adherence to recommendations for the provision of care to asthma patients with first dispensing and follow-up refill encounters in The Netherlands. Data were pharmacists’ self-assessment of adherence to guideline recommendations, independent observations of dispensing encounters and a nationwide questionnaire on pharmacists’ views on the desirable (clinical) necessity of applying guideline recommendations to their patient population. The 21 pharmacists who performed self-assessment judged their adherence concerning inhalation instructions as high. The lowest scores were reported for recommendations to collect additional information on the type of lung disease and for asking patients’ expectations, wishes and concerns. Sixty-eight dispensing encounters were observed. In 83% of the 35 first dispensing observations, inhalation instruction was provided. This percentage was lower (62%) at refill dispensings. During all encounters, pharmacy staff seldom explored patients’ perceptions or responded to patients’ expectations, wishes and concerns. One hundred and four pharmacists completed the feasibility questionnaire. Pharmacists judged that all patients should receive inhalation instruction at first dispensing. They regarded it necessary to check on patients’ expectations, wishes and concerns regarding the treatment for only up to 70% of the patients. More efforts on guideline implementation are needed, especially on follow-up dispensings and on gaining relevant information from patients and other healthcare professionals. Pharmacists still have opportunities to grow in applying a patient-tailored approach and exploring patients’ individual needs, rather than providing practical information.
Predicting the loss of organic archaeological deposits at a regional scale in Greenland
Authors: Hollesen Jørgen , Matthiesen Henning , Fenger-Nielsen Rasmus , Abermann Jakob , Westergaard-Nielsen Andreas , Elberling Bo 
Published: July 2019

AbstractAcross the Arctic, microbial degradation is actively destroying irreplaceable cultural and environmental records that have been preserved within archaeological deposits for millennia. Because it is not possible to survey the many sites in this remote part of the world, new methods are urgently needed to detect and assess the potential degradation. Here, we investigate organic deposits at seven archaeological sites located along the dominating west-east climatic gradient in West Greenland. We show that, regardless of age, depositional history and environmental conditions, all organic deposits are highly vulnerable to degradation. A state-of-the-art model that simulates the effect of future climate change on degradation indicates that 30–70% of the archaeological fraction of organic carbon (OC) could disappear within the next 80 years. This range reflects the variation within the climatic gradient and the future climate scenario applied (RCP 4.5 and RCP 8.5). All archaeological deposits are expected to experience a substantial loss, but the most rapid degradation seems to occur in the continental inland areas of the region, dominated by dry and warm summers. This suggests that organic remains from the Norse Viking Age settlers are especially under threat in the coming years.
Quantification of the flux of tyrosine pathway metabolites during nitisinone treatment of Alkaptonuria
Authors: Milan A. M. , Hughes A. T. , Davison A. S. , Khedr M. , Rovensky J. , Psarelli E. E. , Cox T. F. , Rhodes N. P. , Gallagher J. A. , Ranganath L. R. 
Published: July 2019

AbstractNitisinone decreases homogentisic acid (HGA) in Alkaptonuria (AKU) by inhibiting the tyrosine metabolic pathway in humans. The effect of different daily doses of nitisinone on circulating and 24 h urinary excretion of phenylalanine (PA), tyrosine (TYR), hydroxyphenylpyruvate (HPPA), hydroxyphenyllactate (HPLA) and HGA in patients with AKU was studied over a four week period. Forty AKU patients, randomised into five groups of eight patients, received doses of 1, 2, 4 or 8 mg of nitisinone daily, or no drug (control). Metabolites were analysed by tandem mass spectrometry in 24 h urine and serum samples collected before and after nitisinone. Serum metabolites were corrected for total body water and the sum of 24 hr urine plus total body water metabolites of PA, TYR, HPPA, HPLA and HGA were determined. Body weight and urine urea were used to check on stability of diet and metabolism over the 4 weeks of study. The sum of quantities of urine metabolites (PA, TYR, HPPA, HPLA and HGA) were similar pre- and post-nitisinone. The sum of total body water metabolites were significantly higher post-nitisinone (p < 0.0001) at all doses. Similarly, combined 24 hr urine:total body water ratios for all analytes were significantly higher post-nitisinone, compared with pre-nitisinone baseline for all doses (p = 0.0002 – p < 0.0001). Significantly higher concentrations of metabolites from the tyrosine metabolic pathway were observed in a dose dependant manner following treatment with nitisinone and we speculate that, for the first time, experimental evidence of the metabolite pool that would otherwise be directed towards pigment formation, has been unmasked.
Comparative Brain Morphology of the Greenland and Pacific Sleeper Sharks and its Functional Implications
Authors: Yopak Kara E. , McMeans Bailey C. , Mull Christopher G. , Feindel Kirk W. , Kovacs Kit M. , Lydersen Christian , Fisk Aaron T. , Collin Shaun P. 
Published: July 2019

AbstractIn cartilaginous fishes, variability in the size of the brain and its major regions is often associated with primary habitat and/or specific behavior patterns, which may allow for predictions on the relative importance of different sensory modalities. The Greenland (Somniosus microcephalus) and Pacific sleeper (S.pacificus) sharks are the only non-lamnid shark species found in the Arctic and are among the longest living vertebrates ever described. Despite a presumed visual impairment caused by the regular presence of parasitic ocular lesions, coupled with the fact that locomotory muscle power is often depressed at cold temperatures, these sharks remain capable of capturing active prey, including pinnipeds. Using magnetic resonance imaging (MRI), brain organization ofS.microcephalusandS.pacificuswas assessed in the context of up to 117 other cartilaginous fish species, using phylogenetic comparative techniques. Notably, the region of the brain responsible for motor control (cerebellum) is small and lacking foliation, a characteristic not yet described for any other large-bodied (>3 m) shark. Further, the development of the optic tectum is relatively reduced, while olfactory brain regions are among the largest of any shark species described to date, suggestive of an olfactory-mediated rather than a visually-mediated lifestyle.
Integration of machine learning and mechanistic models accurately predicts variation in cell density of glioblastoma using multiparametric MRI
Authors: Gaw Nathan , Hawkins-Daarud Andrea , Hu Leland S. , Yoon Hyunsoo , Wang Lujia , Xu Yanzhe , Jackson Pamela R. , Singleton Kyle W. , Baxter Leslie C. , Eschbacher Jennifer , Gonzales Ashlyn , Nespodzany Ashley , Smith Kris , Nakaji Peter , Mitchell J. Ross , Wu Teresa , Swanson Kristin R. , Li Jing 
Published: July 2019

AbstractGlioblastoma (GBM) is a heterogeneous and lethal brain cancer. These tumors are followed using magnetic resonance imaging (MRI), which is unable to precisely identify tumor cell invasion, impairing effective surgery and radiation planning. We present a novel hybrid model, based on multiparametric intensities, which combines machine learning (ML) with a mechanistic model of tumor growth to provide spatially resolved tumor cell density predictions. The ML component is an imaging data-driven graph-based semi-supervised learning model and we use the Proliferation-Invasion (PI) mechanistic tumor growth model. We thus refer to the hybrid model as the ML-PI model. The hybrid model was trained using 82 image-localized biopsies from 18 primary GBM patients with pre-operative MRI using a leave-one-patient-out cross validation framework. A Relief algorithm was developed to quantify relative contributions from the data sources. The ML-PI model statistically significantly outperformed (p < 0.001) both individual models, ML and PI, achieving a mean absolute predicted error (MAPE) of 0.106 ± 0.125 versus 0.199 ± 0.186 (ML) and 0.227 ± 0.215 (PI), respectively. Associated Pearson correlation coefficients for ML-PI, ML, and PI were 0.838, 0.518, and 0.437, respectively. The Relief algorithm showed the PI model had the greatest contribution to the result, emphasizing the importance of the hybrid model in achieving the high accuracy.
Decomposition of sediment-oil-agglomerates in a Gulf of Mexico sandy beach
Authors: Bociu Ioana , Shin Boryoung , Wells Wm. Brian , Kostka Joel E. , Konstantinidis Konstantinos T. , Huettel Markus 
Published: July 2019

AbstractSediment-oil-agglomerates (SOA) are one of the most common forms of contamination impacting shores after a major oil spill; and following the Deepwater Horizon (DWH) accident, large numbers of SOAs were buried in the sandy beaches of the northeastern Gulf of Mexico. SOAs provide a source of toxic oil compounds, and although SOAs can persist for many years, their long-term fate was unknown. Here we report the results of a 3-year in-situ experiment that quantified the degradation of standardized SOAs buried in the upper 50 cm of a North Florida sandy beach. Time series of hydrocarbon mass, carbon content, n-alkanes, PAHs, and fluorescence indicate that the decomposition of golf-ball-size DWH-SOAs embedded in beach sand takes at least 32 years, while SOA degradation without sediment contact would require more than 100 years. SOA alkane and PAH decay rates within the sediment were similar to those at the beach surface. The porous structure of the SOAs kept their cores oxygen-replete. The results reveal that SOAs buried deep in beach sands can be decomposed through relatively rapid aerobic microbial oil degradation in the tidally ventilated permeable beach sand, emphasizing the role of the sandy beach as an aerobic biocatalytical reactor at the land-ocean interface.
Incense Burning is Associated with Human Oral Microbiota Composition
Authors: Vallès Yvonne , Inman Claire K. , Peters Brandilyn A. , Wareth Laila Abdel , Abdulle Abdishakur , Alsafar Habiba , Anouti Fatme Al , Dhaheri Ayesha Al , Galani Divya , Haji Muna , Hamiz Aisha Al , Hosani Ayesha Al , Houqani Mohammed Al , Aljunaibi Abdulla , Kazim Marina , Kirchhoff Tomas , Mahmeed Wael Al , Maskari Fatma Al , Alnaeemi Abdullah , Oumeziane Naima , Ramasamy Ravichandran , Schmidt Ann Marie , Vallès Henri , Zaabi Eiman Al , Sherman Scott , Ali Raghib , Ahn Jiyoung , Hayes Richard B. 
Published: July 2019

AbstractIncense burning is common worldwide and produces environmental toxicants that may influence health; however, biologic effects have been little studied. In 303 Emirati adults, we tested the hypothesis that incense use is linked to compositional changes in the oral microbiota that can be potentially significant for health. The oral microbiota was assessed by amplification of the bacterial 16S rRNA gene from mouthwash samples. Frequency of incense use was ascertained through a questionnaire and examined in relation to overall oral microbiota composition (PERMANOVA analysis), and to specific taxon abundances, by negative binomial generalized linear models. We found that exposure to incense burning was associated with higher microbial diversity (p < 0.013) and overall microbial compositional changes (PERMANOVA, p = 0.003). Our study also revealed that incense use was associated with significant changes in bacterial abundances (i.e. depletion of the dominant taxonStreptococcus), even in occasional users (once/week or less) implying that incense use impacts the oral microbiota even at low exposure levels. In summary, this first study suggests that incense burning alters the oral microbiota, potentially serving as an early biomarker of incense-related toxicities and related health consequences. Although a common indoor air pollutant, guidelines for control of incense use have yet to be developed.
Transcriptome and digital gene expression analysis unravels the novel mechanism of early flowering in Angelica sinensis
Authors: Yu Guang , Zhou Yuan , Yu Juanjuan , Hu Xueqin , Tang Ye , Yan Hui , Duan Jinao 
Published: July 2019

AbstractAngelica sinensis (Oliv.) Diels is a widely used medicinal plant mainly originated in Gansu, China. Angelica sinensis is greatly demanded in the clinical practice of Chinese medicine due to its broad pharmacological activities of hematopoietic and anti-inflammatory properties. But, the percentage of early flowering in Angelica sinensis arrives to 20%~30%, which severely affects its quality and quantity. Here, transcriptome profiling and digital gene expression analysis were applied to study the mechanism of early flowering in Angelica sinensis. A total of 49,183,534 clean reads were obtained and assembled into 68,262 unigenes, and 49,477 unigenes (72.5%) could be annotated to a minimum of one database in the Nr, Nt, Swiss-Pro, GO, COG and KEGG. Taking the above transcriptome data as a reference, digital gene expression result showed that 5,094 genes expression level were significant changed during early flowering. These annotated genes offered much information promoting that the biosynthesis of secondary metabolites pathway, the hormone signal transduction pathway, and the transcription regulation system may be closely related to the early flowering phenomenon of Angelica sinensis. Further expression patterns of key genes contribute to early flowering were analyzed using quantitative real-time PCR. The transcriptome result offered important gene expression information about early flowering in Angelica sinensis.
Effects of Temperature and Photoperiod on the Immature Development in Cassida rubiginosa Müll. and C. stigmatica Sffr. (Coleoptera: Chrysomelidae)
Authors: Kutcherov Dmitry , Lopatina Elena B. , Yermakov Stepan 
Published: July 2019

AbstractTortoise beetles (Cassida and related genera) are a large cosmopolitan group that includes several pests of agricultural crops and natural enemies of weeds but their biology and ecology remain poorly known. Using a set of environmental chambers, we address simultaneous effects of temperature and photoperiod on immature development and adult body mass in two European species, C. rubiginosa and C. stigmatica. Consistent with its broader distribution range, the former species is less susceptible to low rearing temperatures, develops faster and has a larger body mass than the latter. However, C. rubiginosa seems to be less adapted to late-season conditions as a short-day photoperiod accelerates its immature development to a lesser extent than it does in C. stigmatica, which nevertheless results in greater larval mortality and slightly but significantly smaller adults. By contrast, in C. stigmatica, which is more likely to encounter late-season conditions due to its slower life cycle, short-day acceleration of development is achieved at no cost to survivorship and final body mass. The experiment with C. stigmatica was repeated during two consecutive years with different methods and the main results proved to be well reproducible. In addition, laboratory results for C. rubiginosa agree with field data from literature.
Modeling vitamin B1 transfer to consumers in the aquatic food web
Authors: Ejsmond M. J. , Blackburn N. , Fridolfsson E. , Haecky P. , Andersson A. , Casini M. , Belgrano A. , Hylander S. 
Published: July 2019

AbstractVitamin B1 is an essential exogenous micronutrient for animals. Mass death and reproductive failure in top aquatic consumers caused by vitamin B1 deficiency is an emerging conservation issue in Northern hemisphere aquatic ecosystems. We present for the first time a model that identifies conditions responsible for the constrained flow of vitamin B1 from unicellular organisms to planktivorous fishes. The flow of vitamin B1 through the food web is constrained under anthropogenic pressures of increased nutrient input and, driven by climatic change, increased light attenuation by dissolved substances transported to marine coastal systems. Fishing pressure on piscivorous fish, through increased abundance of planktivorous fish that overexploit mesozooplankton, may further constrain vitamin B1 flow from producers to consumers. We also found that key ecological contributors to the constrained flow of vitamin B1 are a low mesozooplankton biomass, picoalgae prevailing among primary producers and low fluctuations of population numbers of planktonic organisms.
Isoform-specific Inhibition of N-methyl-D-aspartate Receptors by Bile Salts
Authors: Koch Angela , Bonus Michele , Gohlke Holger , Klöcker Nikolaj 
Published: July 2019

AbstractThe N-methyl-D-aspartate subfamily of ionotropic glutamate receptors (NMDARs) is well known for its important roles in the central nervous system (CNS), e.g. learning and memory formation. Besides the CNS, NMDARs are also expressed in numerous peripheral tissues including the pancreas, kidney, stomach, and blood cells, where an understanding of their physiological and pathophysiological roles is only evolving. Whereas subunit composition increases functional diversity of NMDARs, a great number of endogenous cues tune receptor signaling. Here, we characterized the effects of the steroid bile salts cholate and chenodeoxycholate (CDC) on recombinantly expressed NMDARs of defined molecular composition. CDC inhibited NMDARs in an isoform-dependent manner, preferring GluN2D and GluN3B over GluN2A and GluN2B receptors. Determined IC50 values were in the range of bile salt serum concentrations in severe cholestatic disease states, pointing at a putative pathophysiological significance of the identified receptor modulation. Both pharmacological and molecular simulation analyses indicate that CDC acts allosterically on GluN2D, whereas it competes with agonist binding on GluN3B receptors. Such differential modes of inhibition may allow isoform-specific targeted interference with the NMDAR/bile salt interaction. In summary, our study provides further molecular insight into the modulation of NMDARs by endogenous steroids and points at a putative pathophysiological role of the receptors in cholestatic disease.
Simultaneous determination of 25 pesticides in Zizania latifolia by dispersive solid-phase extraction and liquid chromatography-tandem mass spectrometry
Authors: Xu Feng , Yu Jia-yong , Wang Quan-sheng , Fu Yan , Zhang Hao , Wu Yin-liang 
Published: July 2019

AbstractAn improved quick, easy, cheap, effective, rugged, and safe (QuEChERS) method combined with ultrapressure liquid chromatography tandem mass spectrometric method (UPLC-MS/MS) was developed to simultaneously determine 25 pesticides in Zizania latifolia. The samples were extracted with methanol(MeOH) and 0.1% formic acid (80:20, v/v) and cleaned with C18 absorbent and primary-secondary amine (PSA). LC separation was performed on a BEH C18 UPLC column under the condition of gradient elution with the mobile phase consisted of 0.5% formic acid (10 mM ammonium acetate)/MeOH. External standard calibration method with matrix-matched was used for quantification, and good linearity was obtained over a concentration range of 0.5–100 μg/l, with correlation coefficients greater than 0.9901. The limit of detection (LOD) and the limit of quantitation (LOQ) of the 25 pesticides were in the range of 0.2–1.0 µg/kg and 0.5–3.3 µg/kg, respectively. The recoveries ranged from 72% to 118%, and the relative standard deviations (RSDs) were less than 20%. Thus, the proposed method is suitable for the simultaneous determination of 25 pesticides in Z. latifolia.
Activity of the inferior parietal cortex is modulated by visual feedback delay in the robot hand illusion
Authors: Ismail Mohamad Arif Fahmi Bin , Shimada Sotaro 
Published: July 2019

AbstractThe robot hand illusion (RoHI) is the perception of self-ownership and self-agency of a virtual (robot) hand that moves consistently with one’s own. The phenomenon shows that self-attribution can be established via temporal integration of visual and movement information. Our previous study showed that participants felt significantly greater RoHI (sense of self-ownership and sense of self-agency) when visuomotor temporal discrepancies were less than 200 ms. A weaker RoHI effect (sense of self-agency only) was observed when temporal discrepancies were between 300 and 500 ms. Here, we used functional near-infrared spectroscopy (fNIRS) to investigate brain activity associated with the RoHI under different visual feedback delays (100 ms, 400 ms, 700 ms). We found that the angular and supramarginal gyri exhibited significant activation in the 100-ms feedback condition. ANOVA indicated a significant difference between the 100-ms condition and the other conditions (p < 0.01). These results demonstrate that activity in the posterior parietal cortex was modulated by the delay between the motor command and the visual feedback of the virtual hand movements. Thus, we propose that the inferior parietal cortex is essential for integrating motor and visual information to distinguish one’s own body from others.
MRI coupled with clinically-applicable iron oxide nanoparticles reveals choroid plexus involvement in a murine model of neuroinflammation
Authors: Hubert Violaine , Dumot Chloé , Ong Elodie , Amaz Camille , Canet-Soulas Emmanuelle , Chauveau Fabien , Wiart Marlène 
Published: July 2019

AbstractChoroid plexus (ChPs) are involved in the early inflammatory response that occurs in many brain disorders. However, the activation of immune cells within the ChPs in response to neuroinflammation is still largely unexplored in-vivo. There is therefore a crucial need for developing imaging tool that would allow the non-invasive monitoring of ChP involvement in these diseases. Magnetic resonance imaging (MRI) coupled with superparamagnetic particles of iron oxide (SPIO) is a minimally invasive technique allowing to track phagocytic cells in inflammatory diseases. Our aim was to investigate the potential of ultrasmall SPIO (USPIO)-enhanced MRI to monitor ChP involvement in-vivo in a mouse model of neuroinflammation obtained by intraperitoneal administration of lipopolysaccharide. Using high resolution MRI, we identified marked USPIO-related signal drops in the ChPs of animals with neuroinflammation compared to controls. We confirmed these results quantitatively using a 4-points grading system. Ex-vivo analysis confirmed USPIO accumulation within the ChP stroma and their uptake by immune cells. We validated the translational potential of our approach using the clinically-applicable USPIO Ferumoxytol. MR imaging of USPIO accumulation within the ChPs may serve as an imaging biomarker to study ChP involvement in neuroinflammatory disorders that could be applied in a straightforward way in clinical practice.