bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the sequence variability of polymerization-involved residues in the production of levan- and inulin-type fructooligosaccharides with a levansucrase
Authors: Possiel Christian , Ortiz-Soto Maria Elena , Ertl Julia , Münch Angela , Vogel Andreas , Schmiedel Ramona , Seibel Jürgen 
Published: May 2019

AbstractThe connection between the gut microbiome composition and human health has long been recognized, such that the host-microbiome interplay is at present the subject of the so-called “precision medicine”. Non-digestible fructooligosaccharides (FOS) can modulate the microbial composition and therefore their consumption occupies a central place in a strategy seeking to reverse microbiome-linked diseases. We created a small library of Bacillus megaterium levansucrase variants with focus on the synthesis of levan- and inulin-type FOS. Modifications were introduced at positions R370, K373 and F419, which are either part of the oligosaccharide elongation pathway or are located in the vicinity of residues that modulate polymerization. These amino acids were exchanged by residues of different characteristics, some of them being extremely low- or non-represented in enzymes of the levansucrase family (Glycoside Hydrolase 68, GH68). F419 seemed to play a minor role in FOS binding. However, changes at R370 abated the levansucrase capacity to synthesize levan-type oligosaccharides, with some mutations turning the product specificity towards neo-FOS and the inulin-like sugar 1-kestose. Although variants retaining the native R370 produced efficiently levan-type tri-, tetra- and pentasaccharides, their capacity to elongate these FOS was hampered by including the mutation K373H or K373L. Mutant K373H, for instance, generated 37- and 5.6-fold higher yields of 6-kestose and 6-nystose, respectively, than the wild-type enzyme, while maintaining a similar catalytic activity. The effect of mutations on the levansucrase product specificity is discussed.
Vorstellung eines benutzerfreundlichen Programmes zur biometrischen Fallzahlplanung in klinischen und epidemiologischen Studien
Authors: Geis Berit , Tulka Sabrina , Knippschild Stephanie , Krummenauer Frank 
Published: May 2019

ZusammenfassungHintergrund Für die Darstellung von Studienergebnissen werden von Zeitschriften Konfidenzintervalle eingefordert, da diese anders als Signifikanztests auch die klinische Relevanz der Ergebnisse darlegen. Die in der Planungsphase einer Studie obligatorisch erfolgten biometrischen Fallzahlplanungen werden hingegen in den meisten Fällen über Signifikanztests berichtet, da kaum Methoden zur entsprechenden Planung über Konfidenzintervalle verfügbar sind. Als Resultat sind Planungs- und Ergebnisdarstellung klinischer Studien in vielen Artikeln nicht direkt vergleichbar. Präsentiert werden soll eine neu entwickelte Anwendung, die dem klinischen Benutzer beide Ansätze wechselweise zugänglich macht.Material und Methoden Die Fallzahlplanung über ein Konfidenzintervall erfordert für den in der Studie aufzudeckenden klinisch relevanten Unterschied die Vorgabe einer maximalen halben Intervallbreite, die Planung über einen Signifikanztest die Vorgabe von dessen Mindestpower. Die resultierenden Fallzahlformeln sind für praktisch relevante Fälle kompatibel und unterscheiden sich hauptsächlich in der Vorgabe der beiden Parameter „Breite“ und „Power“; für die speziell klinischen Anwendern eher vertraute Power existieren bekannte Orientierungswerte (meist 80 oder 90%), während sich die Planung über Konfidenzintervalle aufgrund fehlender Richtlinien für die Wahl der halben Breite als schwieriger zugänglich gestaltet. Anhand der Planung einer Studie zur Rotationsstabilität torischer Intraokularlinsen mit bzw. ohne Kapselspannring werden die beiden Zugänge demonstriert.Ergebnisse Es wurde eine Applikation entwickelt, in der beide Fallzahlplanungsansätze für den Anwender zugänglich implementiert sind. Dies ermöglicht im Fall 2-armiger Studiendesigns sowohl einen Vergleich der beiden Zugänge als auch einen Wechsel zwischen diesen. Der Anwender wählt zunächst aus, ob eine Berechnung mittels Signifikanztest oder Konfidenzintervall erfolgen soll, woraufhin die jeweiligen Planungsdaten eingegeben und die daraus resultierende Fallzahl sowie die zum alternativen Ansatz korrespondierende Parametrisierung der Planungsdaten ausgegeben wird.Schlussfolgerung Die entwickelte Applikation ermöglicht eine für klinische Anwender intuitiv zugängliche Fallzahlberechnung für 2-armige Studiendesigns sowohl über Signifikanztests als auch über Konfidenzintervalle.
Update on early thrombus removal for iliofemoral deep vein
thrombosis
Authors: Bækgaard Niels 
Published: May 2019

AbstractThrombus removal has for many years been attractive to investigate. The reasons
were to relieve the acute symptoms with painful swelling and even to avoid
tissue loss, but also with focus to decrease the incidence of late
complications. Surgical procedures in the beginning and systemic thrombolysis
years later were practiced in few dedicated centers. The breakthrough came 30
years ago in order to concentrate the lysis procedure directly into the thrombus
material. This minimal invasive catheter-directed procedure was soon combined
with stenting of the well-known iliac vein compression syndrome. Further
development of this modality has taken place with some adjunctive mechanical
devices primarily to minimize bleeding, which is the major concern of the
methods. Less than ten RCT’s have been published over time, but conclusively
supporting the rationale of early thrombus removal for iliofemoral DVT with
reduction of PTS.
2nd Mexican Consensus of Endovenous Chemical Ablation (Scleorotherapy) 2018
Authors: Vega Rasgado F. , Ángel López Paredes J. , Serralde Gallegos J. A. , Bolaños Celaya D. , Ramírez Cadena C. , Alberto Cavazos Ortega M. , Lira Rodríguez O. , Rendón Elías F. , Contreras Cisneros F. , Lugo Ramírez A. , Jiménez Gorena E. 
Published: May 2019

Abstract
Background Sclerotherapy has been carried out since 1516 and is accepted and performed worldwide according to many references from books and journals. Mexican doctors participated in other consensuses in order to obtain various agreements, references, methods and procedures that are universally accepted to establish this document, including the European consensus of sclerotherapy 2003 and its last revision in 2006, the Clinical practice guidelines for prevention, diagnosis and treatment of chronic venous insufficiency in 2009 in México, the Argentine and Latin American consensus for sclerotherapy in 2012, European sclerotherapy guidelines in chronic venous diseases of 2013,and various literature sources as well. The first Mexican consensus on sclerotherapy published in January 2014 which was reviewed and updated in this document.
Objective Review and update the general bases of Endovenous Chemical Ablation (ECA) also known as Sclerotherapy, according to evidence-based medicine updating the previously published consensus.
Method General questions about Endovenous Chemical Ablation (ECA) were established concerning: indications, contraindications, types of sclerosing agents and the concentrations used, patient position, methods of application, way to prepare and preserve the sclerosants, necessary equipment, injection volumes, differents administration ways, compressive therapy and special comments. To answer these questions the existing literature and the topics in which the reports showed uniformity were consulted. A panel of experts was constituted who provided their personal experience, the answers were formulated in the form of clinical guidelines or general recommendations to provide concrete answers to the specific questions, in some issues we include possible accepted variations.
Sinus pilonidalis – was ist die beste chirurgische Therapie?
Authors: Petersen Sven 
Published: May 2019

Zusammenfassung
Einleitung Der Sinus pilonidalis ist eine meist chronische punktuelle Infektion der behaarten Haut im Bereich von Hautfalten mit der häufigsten Lokalisation im Bereich der Rima ani. Die Erkrankung hat oft erhebliche Auswirkung auf die Lebensqualität der Betroffenen, die Behandlung kann komplex sein und muss dem Patientenbedarf angepasst werden.
Methoden Im Folgenden wird eine Übersicht über die Entstehung sowie die Behandlungsalternativen und deren Besonderheiten gegeben. Dabei werden jeweils die Aspekte frühe postoperative Ergebnisse, Wundheilungsstörung und Langzeitergebnisse dargestellt.
Ergebnisse Der Pathomechanismus der Pilonidalsinus-Erkrankung ist weiterhin nicht abschließend geklärt, es muss auch weiterhin davon ausgegangen werden, dass die Haare in der Rima ani wesentlich zu Entstehung beitragen. Neuere Erkenntnisse weisen jedoch darauf hin, dass die großen Haarbüschel, die immer wieder in der Wundhöhle gefunden werden, am ehesten von der behaarten Kopfhaut stammen. Es lassen sich im Wesentlichen 3 Gruppen der Behandlungsoptionen unterscheiden: Die Standardbehandlung der Exzision und die folgende offene Wundbehandlung hat eine geringe Rezidivrate, weist jedoch erheblichen Aufwand im Rahmen der Wundbehandlung auf und führt dadurch zu langer Krankheitsdauer. Relativ neu in den Fokus geraten sind die lokal destruierenden Behandlungsverfahren, welche die Haut im Wesentlichen erhalten. Wichtige Vertreter sind das sog. Pit Picking, Fistuloskopie und andere Behandlungsformen wie Phenolinjektion oder Laserbehandlung. Bei diesen Behandlungsmöglichkeiten steht die abschließende Bewertung der Ergebnisse noch aus. Die 3. Behandlungsgruppe umfasst die Exzision und plastisch-rekonstruktive Verfahren. Diese Verfahren sind technisch aufwendig, gehen häufig mit stationärer Behandlung einher und führen langfristig jedoch zu relativ guten Ergebnissen.
Schlussfolgerung Die Pilonidalsinus-Behandlung erfordert eine am Patienten orientierte Therapieplanung. Dabei müssen dem Patienten die unterschiedlichen Behandlungsoptionen vermittelt werden und eine Behandlungsempfehlung muss ausgesprochen werden. Hier dient die Exzision und offene Wundbehandlung weiterhin als Standardverfahren, anhand dessen die Vor- bzw. Nachteile der anderen Behandlungsoptionen vermittelt werden können.
Prone Positioning of Patients with Cervical Spine Pathology
Authors: Boyle Sarah L. , Unger Zoe , Kulkarni Vinay , Massicotte Eric M. , Venkatraghavan Lashmi 
Published: May 2019

AbstractPatients with cervical trauma or degenerative disease often require surgical decompression and stabilization in the prone position and are at the risk of secondary neurological injury during this transfer. This review aims to explore the current literature on different methods of positioning patients prone and to identify the safest technique to achieve prone positioning in patients with an unstable cervical spine undergoing posterior cervical spine surgery. We searched the Embase, Medline, and Medline-in Process databases for literature in English related to prone positioning patients with cervical spine pathology undergoing spine surgery. Seventy-three citations were identified as relevant and reviewed in detail with 20 articles being identified as answering the clinical questions posed. Our literature review identified three methods of prone positioning patients with cervical pathology: logroll with manual in-line stabilization (MILS), rotating the patient on a specialized spinal table using a “sandwich and flip” technique, and awake prone positioning. Each of these methods has its own advantages and disadvantages. When comparing the degree of neck movement between positioning techniques, “sandwich and flip” rotation was associated with over 50% reduction in both flexion–extension and axial–lateral rotation as compared to logroll with MILS. Awake self-positioning of a patient is another alternative that allows for rapid neurological assessment after repositioning. A “sandwich and flip” is the safest way to turn a patient with cervical pathology into a prone position for surgery. For cooperative patients, who are physically capable, awake self-positioning is a good alternative.
Hemolytic Anemia following Stent-Graft Transjugular Intrahepatic Portosystemic Shunt Creation
Authors: Kuei Andrew , Mikolajczyk Adam E. , Niemeyer Matthew M. 
Published: May 2019

AbstractTransjugular intrahepatic portosystemic shunt (TIPS) creation is a minimally invasive technique aimed at managing the complications of portal hypertension. Initially performed in the setting of variceal bleeding, the role of TIPS has expanded to treatment of medically refractory ascites, portal hypertensive gastropathy, hepatic hydrothorax, Budd-Chiari syndrome, portal vein thrombus, and hepatorenal syndrome. Potential complications from TIPS are well documented, and include hepatic encephalopathy, hepatic failure, and TIPS dysfunction. Hemolytic anemia is a lesser known complication related to TIPS creation. In this article, a case of hemolytic anemia following TIPS creation using a Viatorr stent-graft in described.
Transplant Hepatic Artery Stenosis: Endovascular Treatment and Complications
Authors: Molvar Christopher , Ogilvie Ross , Aggarwal Deep , Borge Marc 
Published: May 2019

AbstractHepatic artery stenosis (HAS) is an infrequent complication of liver transplant; if left untreated, it can lead to hepatic artery thrombosis with high risk of biliary necrosis and graft loss. HAS is diagnosed with screening Doppler ultrasound, together with computed tomography angiography and magnetic resonance angiography. Endovascular treatment with angioplasty ± stent placement is safe and effective with infrequent major complications; however, when complications occur, they can devastate long-term graft survival. Herein, we present two cases of HAS treated with balloon angioplasty with resultant major complications requiring operative intervention.
Managing Arrow-Trerotola Percutaneous Thrombolytic Device Complications
Authors: Simpson Russell O. , Gaba Ron C. , Lipnik Andrew J. 
Published: May 2019

AbstractDialysis access interventions are frequently performed by interventional radiologists. Several commercially available percutaneous thrombolytic devices can help restore patency to thrombosed arteriovenous access circuits. The Arrow-Trerotola Percutaneous Thrombolytic Device is one such device, and has a long track record of safe and effective use. However, like any medical device, complications can occur during its use. This article describes three complications and associated management strategies utilizing fundamental interventional radiology techniques of balloon tamponade, stent placement, and snare mediated foreign body retrieval.
Cystic Artery Hemorrhage after Cholecystostomy Catheter Exchange Treated with Transcatheter Embolization
Authors: Sada David M. , Metwalli Zeyad A. 
Published: May 2019

AbstractThe pathogenesis of formation of cystic artery pseudoaneurysms is not well understood, but is thought to result from erosion of the cystic artery wall from the adjacent inflammatory process associated with acute or chronic cholecystitis. The presented case discusses an incident of hemorrhage from a cystic artery pseudoaneurysm resulting in a large perihepatic hematoma as well as hemobilia, which developed after a routine cholecystostomy catheter exchange. This was treated with transcatheter embolization using ethylene-vinyl alcohol copolymer.
Case of Acute Onset Postpartum Paraplegia: Spontaneous Spinal Epidural Hematoma–A Rare Entity
Authors: Pandey Praveen Kumar , Pawar Inder , Singh Harsimar 
Published: May 2019

Study Design Present study is a case report.
Objective This study was to present a rare case of acute postpartum paraplegia due to spontaneous spinal epidural hematoma (SSEH).
Background SSEH with incidence rate of 0.1 per 100,000 per year is an extremely rare cause of sudden onset neurological deficit in postpartum patients with no predisposing factors or intrapartum factor causing SSEH resulting in paraplegia.
Material and Methods We hereby present our case of acute onset postpartum paraplegia which on magnetic resonance imaging (MRI) shows epidural hematoma around thoracic 12 to lumbar 2 (T12–L2) vertebral regions with adjacent cord changes. MRI is the investigation of choice in such cases which helps in proper timely management of patient. We planned the patient for surgical decompression of hematoma.
Results Patient showed rapid reversal of neurological symptoms in postoperative period.
Conclusions Acute onset postpartum paraplegia in a healthy female with no significant past history, predisposing factors or intrapartum factors may be caused by SSEH and it should be managed on emergency basis as early and proper treatment has an excellent prognosis as seen in our case.
Radiosensitivity of colorectal cancer to 90Y and the radiobiological implications for radioembolisation therapy
Authors: Lee Boon Q , Abbott Elliot M , Able Sarah , Thompson James M , Hill Mark A , Kartsonaki Christiana , Vallis Katherine A , Falzone Nadia 
Published: May 2019

Abstract
Approximately 50% of all colorectal cancer (CRC) patients will develop metastasis to the liver. 90Y selective internal radiation therapy (SIRT) is an established treatment for metastatic CRC. There is still a fundamental lack of understanding regarding the radiobiology underlying the dose response. This study was designed to determine the radiosensitivity of two CRC cell lines (DLD-1 and HT-29) to 90Y β
− radiation exposure, and thus the relative effectiveness of 90Y SIRT in relation to external beam radiotherapy (EBRT).
A 90Y-source dish was sandwiched between culture dishes to irradiate DLD-1 or HT-29 cells for a period of 6 d. Cell survival was determined by clonogenic assay. Dose absorbed per 90Y disintegration was calculated using the PENELOPE Monte Carlo code. PENELOPE simulations were benchmarked against relative dose measurements using EBT3 GAFchromic™ film. Statistical regression based on the linear-quadratic model was used to determine the radiosensitivity parameters

and

using R. These results were compared to radiosensitivity parameters determined for 6 MV clinical x-rays and 137Cs γ-ray exposure. Equivalent dose of EBRT in 2 Gy (

) and 10 Gy (

) fractions were derived for 90Y dose.
HT-29 cells were more radioresistant than DLD-1 for all treatment modalities. Radiosensitivity parameters determined for 6 MV x-rays and 137Cs γ-ray were equivalent for both cell lines. The

ratio for 90Y β
−-particle exposure was over an order of magnitude higher than the other two modalities due to protraction of dose delivery. Consequently, an 90Y SIRT absorbed dose of 60 Gy equates to an

of 28.7 and 54.5 Gy and an

of 17.6 and 19.3 Gy for DLD-1 and HT-29 cell lines, respectively.
We derived radiosensitivity parameters for two CRC cell lines exposed to 90Y β
−-particles, 6 MV x-rays, and 137Cs γ-ray irradiation. These radiobiological parameters are critical to understanding the dose response of CRC lesions and ultimately informs the efficacy of 90Y SIRT relative to other radiation therapy modalities.
Relationship Quality Among Older Cohabitors: A Comparison to Remarrieds
Authors: Wright Matthew R 
Published: May 2019

Abstract

Objectives
Later life marital patterns have undergone shifts over the past few decades, including a rapid growth of cohabiting unions. Despite the increase in older adult cohabitation, research on this population has been slow to keep up. Intimate relationships are linked to well-being and relationship quality is especially important because high-quality relationships offer a number of benefits for well-being, whereas poor-quality relationships often are detrimental. This study compares cohabiting and remarried individuals on two measures of relationship quality.

Method
Using data from the 2010 and 2012 Health and Retirement Study, I investigate the positive and negative relationship quality of cohabitors relative to their remarried counterparts and whether the association of union type and relationship quality varies by race.

Results
Across both positive and negative relationship quality, I found few differences between cohabiting and remarried individuals. Black cohabitors report higher positive relationship quality than remarrieds, whereas White cohabitors and remarrieds do not differ.

Discussion
These findings suggest that cohabiting unions and remarriages are comparable among White older adults, but that Black cohabitors may gain more in terms of positive relationship quality than their remarried counterparts.
A haplotype-resolved draft genome of the European sardine (Sardina pilchardus)
Authors: Louro Bruno , De Moro Gianluca , Garcia Carlos , Cox Cymon J , Veríssimo Ana , Sabatino Stephen J , Santos António M , Canário Adelino V M 
Published: May 2019

Abstract

Background
The European sardine (Sardina pilchardus Walbaum, 1792) is culturally and economically important throughout its distribution. Monitoring studies of sardine populations report an alarming decrease in stocks due to overfishing and environmental change, which has resulted in historically low captures along the Iberian Atlantic coast. Important biological and ecological features such as population diversity, structure, and migratory patterns can be addressed with the development and use of genomics resources.

Findings
The genome of a single female individual was sequenced using Illumina HiSeq X Ten 10x Genomics linked reads, generating 113.8 gigabase pairs of data. Three draft genomes were assembled: 2 haploid genomes with a total size of 935 megabase pairs (N50 103 kilobase pairs) each, and a consensus genome of total size 950 megabase pairs (N50 97 kilobase pairs). The genome completeness assessment captured 84% of Actinopterygii Benchmarking Universal Single-Copy Orthologs. To obtain a more complete analysis, the transcriptomes of 11 tissues were sequenced to aid the functional annotation of the genome, resulting in 40,777 genes predicted. Variant calling on nearly half of the haplotype genome resulted in the identification of &gt;2.3 million phased single-nucleotide polymorphisms with heterozygous loci.

Conclusions
A draft genome was obtained, despite a high level of sequence repeats and heterozygosity, which are expected genome characteristics of a wild sardine. The reference sardine genome and respective variant data will be a cornerstone resource of ongoing population genomics studies to be integrated into future sardine stock assessment modelling to better manage this valuable resource.