bluuebunny/crossref_metadata_2025_split huggingface dataset:
FSTL1 Can Be a Promising Target in TMJ Osteoarthritis via Regulating Chondrocyte Mitophagy and Apoptosis
Authors: Li Fangjie , Qian Weixiang , Wang Jiayao , Gao Minghua 
Published: November 2024

Background:
Previous studies have shown that follistatin-like protein 1 (FSTL1) is elevated in the synovial fluid of osteoarthritis and whether it is associated with disease development progress in cartilage degeneration is still unclear. The experiment was performed to explore the effect and mechanism of FSTL1 on chondrocyte degeneration and its further impaction in osteoarthritis as well as its treatment method.

Methods:
The patients who were diagnosed with temporomandibular joint (TMJ) disc displacement and osteoarthritis (OA) group was divided into 2 groups, anterior disc displacement (ADD) without bone resorption and ADD with bone resorption group according to the radiologic examination. The ELISA kit was used to determine the expression level of FSTL1 in patients TMJ environment. The function of FSTL1 in promoting chondrocyte degeneration was tested by quantitative reverse transcription polymerase chain reaction (Rt-qPCR) and western blot. The chondrocyte apoptosis and mitophagy were further test by flow cytometry and mitosox staining by upregulating and downregulating of FSTL1. In the end, the effectiveness of regulating FSTL1 in OA procedure was further validated by hematoxylin-eosin (HE), safranin O, and immunohistochemical (IHC) staining in vivo.

Results:
There were 56 samples collected from the patients were included into this study. According to the ELISA results, FSTL1 expression levels of ADD without bone resorption groups were significantly lower than that in ADD with bone resorption group. Furthermore, the rate of cell apoptosis cells and the mitophagy procedure were highly activated when FSTL1 was upregulated. The morphology analysis of mitochondria showed significant changes when FSTL1 was highly upregulated in vitro. The in vivo and in vitro experiments showed that suppressing FSTL1 could alleviate the cartilage degeneration in TMJ OA progression.

Conclusions:
To sum up, upregulated expression level of FSTL1 in synovial fluid promoted the progression of TMJ OA by upregulating accelerating the chondrocyte apoptosis and mitophagy, and suppressing the FSTL1 in TMJ can rescue the OA progression. Therefore, it may be a promising result to consider the FSTL1 as a therapeutic target in the future.
Outcomes of Reconstructing Large Oncosurgical Defects of the Lateral Face in Older Cutaneous Cancer Patients Using Cervicofacial and Cervicothoracic Flaps
Authors: Chen Wei-liang , Huang Zi-xian , Zhou Bin , Liao Juan-kun , Chen Rui 
Published: November 2024

Background:
Older patients with cutaneous cancer often have comorbidities. Tumor resection and defect reconstruction in these patients are challenging.

Aims:
To evaluate the safety and feasibility of cervicofacial (CFF) and cervicothoracic (CTF) flaps in the reconstruction of large oncosurgical defects in the lateral facial region of older patients.

Materials and Methods:
The study enrolled 36 patients with facial cutaneous cancers (age range, 65–94 years). They were divided into CFF and CTF groups in the facial region and in the neck. The clinical stages were I, II, and III in 6, 21, and 9 patients, respectively. The lateral face was divided into 3 aesthetic units: forehead, cheeks, and neck. Postoperative complications were scored using the Clavien-Dindo classification.

Results:
The CFF was used to reconstruct oncosurgical defects in 8 foreheads and 16 cheeks; the CFF was used to reconstruct defects in 4 cheeks and 8 necks. The median sizes of skin defects in the CFF and CTF groups were 3.9×4.4 and 6.8×7.7 cm, respectively. There were no full flap failures. The Clavien-Dindo grades were I, II, and IIIa in 20, 9, and 2 patients, respectively, in the CFF and 0, 2, and 3 in the CTF group. Twenty-nine patients were alive without disease, 5 patients were alive with disease, and 2 patients had died of local recurrence or distant metastases.

Conclusions:
The CFF and CTF are simple, reliable, and excellent methods for reconstructing large oncosurgical defects in the lateral face of older patients with cutaneous cancer.
Alpha–Delta Ratio for Detection of Cerebral Injury and Stroke in Pediatric Extracorporeal Membrane Oxygenation
Authors: Sansevere Arnold J. , DiBacco Melissa L. , Cavan Kelly , Rotenberg Alexander 
Published: November 2024

Introduction:
To assess the alpha–delta ratio (ADR) as a biomarker for cerebral injury and stroke in pediatric extracorporeal membrane oxygenation (ECMO).

Methods:
Retrospective study of children aged >44 weeks gestation to 21 years monitored with continuous electroencephalography during ECMO. The interhemispheric ADR difference between the left and right hemisphere was calculated per hour. A t-test was performed comparing the mean interhemispheric difference between controls and patients with cerebral injury at set intervals (i.e., 1, 3, 6, 9, 12, and 24 hours) from the start of continuous electroencephalography. Injury was established if confirmed by imaging on the same day as ECMO cannulation and acquired if confirmed the day after ECMO or later. Analysis of variance was performed to compare the mean interhemispheric difference in the ADR among control patients to those with early-acquired and late-acquired injury at 24 hours.

Results:
We included 49 patients with a median age of 3.4 years (interquartile range [1–10.4]), 47% (23/49) were male, and 73% (36/49) had cardiac arrest. Cerebrovascular injury was detected in 45% (22/49), with focal stroke in 82% (18/22). A significant difference was seen between control patients compared with cerebrovascular injury after 6 hours of continuous electroencephalography (0.016 vs. 0.042) (mean interhemispheric ADR difference) (P = 0.03). Analysis of variance of control patients to early- and late-acquired injury at 24 hours showed a significant difference (P = 0.03).

Conclusions:
The ADR is a reliable metric to detect in-ECMO cerebral injury and stroke. Further study is needed to automate and assess this metric for real-time detection of stroke in ECMO.
Enhancedin vitroaggregation, but not phase separation, of TDP-43 and its C-terminal fragments generate deep-blue autofluorescence
Authors: Saravanan Preethi , Bharathi Vidhya , Veerabhadraswamy Priyadarshini , Patel Basant K 
Published: November 2024

AbstractAs misfolding and aggregation of the RNA/DNA-binding protein, TDP-43, are linked to devastating TDP-43 proteinopathies like amyotrophic lateral sclerosis (ALS), distinction of the nature of the aggregated TDP-43 species being liquid-like non-pathogenic or solid-like pathogenic is important for mechanistic elucidation and therapeutic targeting. Here, we examined ifin vitroenhancement of the TDP-43 aggregation can generate or enhance intrinsic deep-blue autofluorescence (dbAF) previously reported for a few other protein aggregates and whether dbAF is emitted by all or only liquid-like or solid-like TDP-43 aggregates. Using thioflavin-T fluorescence, turbidimetry, atomic force microscopy and fluorescence microscopy of Alexa Fluor-labelled protein, we first tested thein vitroenhancement of the aggregation of the full-length TDP-43 and its two C-terminal fragments (CTFs), TDP-432C(aa: 193-414) and the TDP-43-low complexity domain (LCD) (aa: 274-414). We find that presence of metal ions, Zn2+or Mn2+, that are also linked to ALS-associated metal dyshomeostasis, or addition of a kosmotropic anion, SO42-, enhance thein vitrosolid-like aggregations of the full-length TDP-43 and TDP-432Cthat also concurrently enhance emission of dbAF. In contrast, Alexa fluor-633-labeled-TDP-43-LCD underwent a quick phase separation into globular structures in presence of Zn2+ions and the phase-separated species failed to emit dbAF but upon further incubation when matured into solid-like irregular, but non-amyloid nature aggregates, it emitted dbAF. Strikingly, we find that the TDP-43 aggregates of both amyloid and non-amyloid nature, but not the oligomers or the phase-separated droplets of TDP-43, manifest dbAF. Overall, the observedin vitroenhancement of aggregation leading to concurrent enhancement of dbAF can enable a label-free easy detection and may facilitate distinguishing of potentially pathogenic versus non-pathogenic TDP-43 aggregates.
Functional split-tRNA: a new perspective on codon decoding mechanism
Authors: Mureev Sergey , Wu Yue , Cui Zhenling , Alexandrov Kirill 
Published: November 2024

AbstractThe translation machinery must rapidly and accurately process all codon triplets despite large differences in the stability of codon:anticodon duplexes. The constrained structure and intramolecular cooperativity of tRNA complicate understanding how its structural elements influence the thermodynamics and kinetics of the selection process. Specifically, it remains unclear whether codon:anticodon complex stability controls kinetics of tRNA selection beyond the codon recognition step. To address this we engineered fully functional split-tRNAs with a dangling anticodon triplet instead of an anticodon loop. Using this tool, we demonstrated that codon-anticodon complex stability is primarily influenced by the dipole moments of adjacent nucleobases and does not control the rate of GTP hydrolysis by EF-Tu. We conclude that the codon-anticodon minihelix functions as a passive steric gate of decoding site closure.
Global Neuron Shape Reasoning with Point Affinity Transformers
Authors: Troidl Jakob , Knittel Johannes , Li Wanhua , Zhan Fangneng , Pfister Hanspeter , Turaga Srinivas 
Published: November 2024

AbstractConnectomics is a subfield of neuroscience that aims to map the brain’s intricate wiring diagram. Accurate neuron segmentation from microscopy volumes is essential for automating connectome reconstruction. However, current state-of-the-art algorithms use image-based convolutional neural networks that are limited to local neuron shape context. Thus, we introduce a new framework that reasons over global neuron shape with a novel point affinity transformer. Our framework embeds a (multi-)neuron point cloud into a fixed-length feature set from which we can decode any point pair affinities, enabling clustering neuron point clouds for automatic proofreading. We also show that the learned feature set can easily be mapped to a contrastive embedding space that enables neuron type classification using a simple KNN classifier. Our approach excels in two demanding connectomics tasks: proofreading segmentation errors and classifying neuron types. Evaluated on three benchmark datasets derived from state-of-the-art connectomes, our method outperforms point transformers, graph neural networks, and unsupervised clustering baselines.1
Computational generation of tailored radionuclide libraries for alpha-particle and gamma-ray spectrometry
Authors: Jang Jaewoong 
Published: November 2024

Radionuclide identification is a radioanalytical method employed in various scientific disciplines that utilize alpha-particle or gamma-ray spectrometric assays, ranging from astrophysics to nuclear medicine. Radionuclide libraries in conventional radionuclide identification systems are crafted in a manual fashion, accompanying labor-intensive and error-prone user tasks and hindering library customization. This research presents a computational algorithm and the architecture of its dedicated software that can automatically generate tailored radionuclide libraries. Progenitor-progeny recurrence relations were modeled to enable recursive computation of radionuclide subsets. This theoretical concept was incorporated into open-source software called and validated against four actinide decay series and 12 radioactive substances, including a uranium-glazed legacy Fiestaware, natural uranium and thorium sources, a Ra226 sample, and the medical radionuclides Ac225, Lu177, and Tc99m. The developed algorithm yielded radionuclide libraries for all the tested specimens within minutes, demonstrating its efficiency and applicability across diverse scenarios. The proposed approach introduces a framework for computerized radionuclide library generation, thereby trivializing library-driven radionuclide identification and facilitating the spectral recognition of unregistered radionuclides in radiation spectrometry.

Published by the American Physical Society
2024
A novel layered structure of the heterometallic oxalate compound [NH2(CH3)2]2[NaFe(C2O4)3]·0.33NH(CH3)2·0.33H2O: synthesis, crystal structure and thermal decomposition
Authors: Kherfi Hamza , Benhacine Mohamed Al Amine , Hamadene Malika 
Published: November 2024

The synthesis, single-crystal X-ray structure determination and thermal analysis are reported for a novel heteronuclear oxalate compound synthesized from a mixture of Fe and Na salts, oxalic acid and N,N-dimethylformamide (DMF) in aqueous solution. The new metallooxalate compound was obtained and identified as a dimethylammonium tris(oxalato)ferrate(III), namely, poly[[bis(dimethylammonium) [tri-μ-oxalato-sodium(I)iron(III)]]–dimethylamine–water (3/1/1)], [NH2(CH3)2]2[NaFe(C2O4)3]·0.33NH(CH3)2·0.33H2O, which crystallizes in the orthorhombic noncentrosymmetric space group C2221. In this novel structure, each Fe atom is hexacoordinated by three non-equivalent bidentate oxalate ligands, while the four Na atoms adopt different coordination numbers, i.e. 6, 7 and 8. The structure consists of bimetallic anionic A layers, {[NaFe(C2O4)3]2−}
n
, displaying a layered structure with infinitely linked Fe2Na2 tetramers on the ab plane. Two kinds of bimetallic parallel layers (A1 and A2) are present alternately and are found to be staggered, while only the A2 layer is crossed by a twofold axis parallel to the a axis through two Na atoms. The [NH2(CH3)2]+ (HDMA) cations occupy the voids between the anionic layers, while the free molecules, i.e. NH(CH3)2 (DMA) and H2O, are located between two different anionic layers. In addition to ionic bonds, the stability of the structure is ensured by hydrogen-bond interactions involving the oxalate ligands and the nitrogenous and water molecules. The layered structure appears to be different in the family of oxalate-bridged NaI–FeIII compounds. It is in agreement with the predicted 2D or layered structure of bimetallic complexes containing anionic tris(oxalato)metallate(III) with the [XR
4]+ counter-ion template (X = N, P or S, and R = alkyl group or H). The thermal decomposition of the compound shows the final residual product to be NaFeO2.
The influence of product availability and social media on green food product purchase intention
Authors: Sharma Latika , Trivedi Mridul , Bagdi Himanshu , Bulsara Hemantkumar P. 
Published: November 2024

PurposeThe present research investigates the role of product availability, environmental concern, and social media concerning the intention to purchase green food products by utilising the extended theory of planned behaviour framework in a developing economy.Design/methodology/approachA self-administered questionnaire collected information from 412 adults and educated consumers from Vadodara city in India. The questionnaire items were used to collect data from previous studies and further validated using confirmatory factor analysis. The data were further analysed using partial least square-structural equation modelling.FindingsThe study findings indicated that attitude and perceived behavioural control impact the purchase intention regarding green food products, while subjective norms were found not supportive. At the same time, product availability influences perceived behavioural control and increases the volitional control amongst consumers. Moreover, social media positively impacts the theory of planned behaviour’s key constructs.Research limitations/implicationsThe outcomes of the present study provide marketing managers with enhanced insight into the relationship between consumers' perception and green food product consumption in the context of social media influence. The findings could help green food producers evaluate the extent of consumers' intentions to buy their products in developing countries.Originality/valueTo the best of the researchers’ knowledge, the present research is amongst the pioneers in India that focuses on the variables of the theory of planned behaviour, which led to knowing the role of green food availability and social media influence in green food products. At the same time, the focus on purchase intention concerning green food products adds to the study’s uniqueness.
Exploring the motives of healthy eating choices: a netnographic study
Authors: Muhammad Asif , Ayyub Samia 
Published: November 2024

PurposeThere is a growing demand for healthy food across multicultural societies, and there are few exploratory studies that examined the motivations behind healthy eating. The purpose of this study is to explore the motives and understand the perceptions of healthy eating and how the online community conceptualizes their healthy eating.Design/methodology/approachData were collected from the online community, such as posts and comments on YouTube channels, Facebook, Twitter and blogs using the hashtags (#healthyeating, #healthylifestyle, #foodforyourhealth, #culinarymedicine, #functionalfood and #nutrition). Content related to healthy eating was transcribed manually. Consumer motives regarding healthy food choices were explored using qualitative data analysis software using thematic analysis and applying Kozinet’s steps for data analysis. By examining communication on YouTube, Twitter, Facebook and blogs, this online study will help us to understand the motives behind healthy food choices.FindingsRespondents appeared to rate food goods’ healthiness on a spectrum. Healthy eating can be conceptualized at both the concrete and abstract representation levels. The themes that emerged through thematic analysis were healthy lifestyles, culinary medicine and the perceived benefits of food.Practical implicationsThis study will guide policymakers and marketers of food products on how to develop strategies while making food products for multicultural societies. Social networking platforms are used to disseminate information on healthy eating to the online community.Originality/valueThe results that were found express the need for healthy food as well as health-related problems and diseases, and they reflect subjects of interest. Social networking sites give healthcare professionals a forum to interact with and educate the online community about nutrition and health. A health care provider can actively participate in online discussion to establish rapport, respond to questions and serve as a reliable source of factual information.
Can regional polycentric spatial structure narrow the urban–rural income gap? Evidence from China
Authors: Zhou Qiuyang 
Published: November 2024

PurposePolycentricity has become a crucial spatial development strategy. This paper aims to clarify the impact and mechanism of polycentric spatial structure on the urban–rural income gap, provide better countermeasures to narrow it and make development suggestions for cities of different scales.Design/methodology/approachBased on the panel data of Chinese cities from 2010 to 2019, this paper empirically examines the specific impact of polycentric spatial structure on the urban–rural income gap by utilizing a two-way fixed effects model.FindingsThe results show that the polycentric spatial structure is favorable for reducing the urban–rural income gap, and upgrading the industrial structure and improving transportation infrastructure are important mechanisms. Moreover, the role of polycentric spatial structure is more significant in large cities. However, in small and medium-sized cities, the role of polycentric spatial structure is not significant.Research limitations/implicationsThe limitations of this study include two main aspects. One is that this paper only analyzes the mediating effect of industrial structure and transport infrastructure, and we do not examine other mechanisms. The second is that due to data availability, this paper uses data from 2010 to 2019, and the research period can be further extended in future studies.Practical implicationsThis study has policy implications for building a polycentric city network and also provides insights into the planning of cities of different scales.Social implicationsThe polycentric spatial structure is important for narrowing the urban–rural income gap, especially in large cities. Improvement of transportation infrastructure and upgrading of industrial structure are important mechanisms. However, the polycentric spatial structure does not play a significant role in small and medium-sized cities. Thus, the polycentric spatial structure is not suitable for every kind of city. Relevant departments should carry out urban planning according to local conditions.Originality/valueAs a critical concern of urban planning, polycentricity significantly affects the urban–rural income gap. Relevant studies have analyzed the impact of polycentric spatial structure on regional income disparity, and few studies have focused on the relationship between polycentric spatial structure and the urban–rural income gap. Using city panel data in China, this paper explores the impact of polycentric spatial structure on the urban-rural income gap. Meanwhile, we test the mediating role of transportation infrastructure improvement and industrial structure upgrading. This study broadens the analysis of the urban–rural income gap’s influencing factors and provides a path reference for narrowing the urban–rural income gap.
Dissecting the provincial relative poverty dynamics in China: a new four-dimensional decomposition approach
Authors: Sun Weihong , Wang Manding , Liu Ding 
Published: November 2024

PurposeThis study investigates relative poverty in China against its rapid economic growth and poverty reduction efforts, aiming to understand the impact of evolving poverty lines and rural-urban disparities.Design/methodology/approachUtilizing micro-survey data and a novel four-dimensional decomposition method, the research decomposes provincial relative poverty changes in China into poverty line and income distribution effects. The poverty line effect is further broken down into the extensive and intensive components, and the impact of income distribution into the growth and inequality effects, offering a detailed examination of their roles in urban and rural contexts.FindingsThe findings indicate that economic growth significantly mitigates the incidence, depth and severity of relative poverty across both settings, whereas income inequality exacerbates these aspects. Additionally, changes in the poverty line through the extensive and intensive effects markedly elevate relative poverty levels. Despite overall reductions, rural areas continue to face higher poverty rates than urban regions, emphasizing the necessity for targeted poverty alleviation policies.Originality/valueThis paper provides a novel perspective by dissecting China’s relative poverty dynamics through a four-dimensional analysis, highlighting the importance of considering economic growth, income inequality and the poverty line adjustments. It underlines the need for policies that specifically address the nuances of relative poverty, including the persistent rural-urban divide, contributing valuable insights for more effective poverty alleviation strategies.