bluuebunny/crossref_metadata_2025_split huggingface dataset:
Continuous Flow Synthesis of a Zr Magnetic Framework Composite for Post‐Combustion CO2 Capture
Authors: He Brandon , Sadiq Muhammad Munir , Batten Michael P. , Suzuki Kiyonori , Rubio‐Martinez Marta , Gardiner James , Hill Matthew R. 
Published: July 2019

AbstractMetal‐organic frameworks (MOFs) have an unprecedented ability to store gas molecules, however energy efficient regeneration remains challenging. Use of magnetic induction to aid this shows promise, but economical synthesis of the requisite composites is unresolved. Continuous flow chemistry has been reported as a rapid and reliable method of MOF synthesis, delivering step‐change improvements in space time yields (STY). Here the scalable production of nanomaterials suitable for regeneration by magnetic induction is demonstrated. The zirconium MOF composite, MgFe2O4@UiO‐66‐NH2 is prepared using continuous flow chemistry resulting in a material of comparable performance to its batch counterpart. Upscaling using flow chemistry gave STY >25 times that of batch synthesis. Magnetic induced regeneration using this mass produced MFC for carbon capture is then demonstrated.
Tumorigenic effects of TLX overexpression in HEK 293T cells
Authors: Parris Toshima Z. , Vizlin‐Hodzic Dzeneta , Salmela Susanne , Funa Keiko 
Published: July 2019

AbstractBackgroundThe human orphan receptor TLX (NR2E1) is a key regulator of neurogenesis, adult stem cell maintenance, and tumorigenesis. However, little is known about the genetic and transcriptomic events that occur following TLX overexpression in human cell lines.AimsHere, we used cytogenetics and RNA sequencing to investigate the effect of TLX overexpression with an inducible vector system in the HEK 293T cell line.Methods and resultsConventional spectral karyotyping was used to identify chromosomal abnormalities, followed by fluorescence in situ hybridization (FISH) analysis on chromosome spreads to assess TLX DNA copy number. Illumina paired‐end whole transcriptome sequencing was then performed to characterize recurrent genetic variants (single nucleotide polymorphisms (SNPs) and indels), expressed gene fusions, and gene expression profiles. Lastly, flow cytometry was used to analyze cell cycle distribution. Intriguingly, we show that upon transfection with a vector containing the human TLX gene (eGFP‐hTLX), an isochromosome forms on the long arm of chromosome 6, thereby resulting in DNA gain of the TLX locus (6q21) and upregulation of TLX. Induction of the eGFP‐hTLX vector further increased TLX expression levels, leading to G0‐G1 cell cycle arrest, genetic aberrations, modulation of gene expression patterns, and crosstalk with other nuclear receptors (AR, ESR1, ESR2, NR1H4, and NR3C2). We identified a 49‐gene signature associated with central nervous system (CNS) development and carcinogenesis, in addition to potentially cancer‐driving gene fusions (LARP1‐CNOT8 and NSL1‐ZDBF2) and deleterious genetic variants (frameshift insertions in the CTSH, DBF4, POSTN, and WDR78 genes).ConclusionTaken together, these findings illustrate that TLX may play a pivotal role in tumorigenesis via genomic instability and perturbation of cancer‐related processes.
Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9
Authors: Giuliano Christopher J. , Lin Ann , Girish Vishruth , Sheltzer Jason M. 
Published: July 2019

AbstractCRISPR/Cas9 technology enables the rapid generation of loss‐of‐function mutations in a targeted gene in mammalian cells. A single cell harboring those mutations can be used to establish a new cell line, thereby creating a CRISPR‐induced knockout clone. These clonal cell lines serve as crucial tools for exploring protein function, analyzing the consequences of gene loss, and investigating the specificity of biological reagents. However, the successful derivation of knockout clones can be technically challenging and may be complicated by multiple factors, including incomplete target ablation and interclonal heterogeneity. Here, we describe optimized protocols and plasmids for generating clonal knockouts in mammalian cell lines. We provide strategies for guide RNA design, CRISPR delivery, and knockout validation that facilitate the derivation of true knockout clones and are amenable to multiplexed gene targeting. These protocols will be broadly useful for researchers seeking to apply CRISPR to study gene function in mammalian cells. © 2019 The Authors.
Performance improvement of wireless secret key generation with colored noise for IoT
Authors: Soni Ankit , Upadhyay Raksha , Kumar Abhay 
Published: July 2019

SummaryAchieving security in the Internet of things (IoT) networks by generating symmetric keys from the wireless channel parameters like received signal strength (RSS) is a promising approach. Despite the easy acquisition of the RSS signal, RSS‐based security is less explored for IoT. In this work, we analyze the performance of RSS‐based wireless physical layer key generation with correlated colored noise components and proposed a low complexity filtering approach to improve the performance for the IoT network. We started with providing a survey of various recent researches related to RSS‐based key generation and also discussed correlated colored noise components with a few of the recent works considering them. Further, we analyze various colored noise components in the time domain by the Allan variance and Ljung‐Box test. Furthermore, we develop a key generation model and proposed a moving window averaging‐based filtering followed by Lloyd max quantization to improve the BDR performance, degraded due to the presence of correlated colored noise components. The simulation results show that the proposed preprocessing technique has a considerable improvement in the BDR performance, and the keys generated have sufficient randomness, which is verified by NIST test.
Peramorphosis, an evolutionary developmental mechanism in neotropical bat skull diversity
Authors: Camacho Jasmin , Heyde Alexander , Bhullar Bhart‐Anjan S. , Haelewaters Danny , Simmons Nancy B. , Abzhanov Arhat 
Published: July 2019

AbstractBackgroundThe neotropical leaf‐nosed bats (Chiroptera, Phyllostomidae) are an ecologically diverse group of mammals with distinctive morphological adaptations associated with specialized modes of feeding. The dramatic skull shape changes between related species result from changes in the craniofacial development process, which brings into focus the nature of the underlying evolutionary developmental processes.ResultsIn this study, we use three‐dimensional geometric morphometrics to describe, quantify, and compare morphological modifications unfolding during evolution and development of phyllostomid bats. We examine how changes in development of the cranium may contribute to the evolution of the bat craniofacial skeleton. Comparisons of ontogenetic trajectories to evolutionary trajectories reveal two separate evolutionary developmental growth processes contributing to modifications in skull morphogenesis: acceleration and hypermorphosis.ConclusionThese findings are consistent with a role for peramorphosis, a form of heterochrony, in the evolution of bat dietary specialists.
Thresholds are in the eye of the beholder: plants and wildlife respond differently to short‐term cattle corrals
Authors: Veblen Kari E. , Porensky Lauren M. 
Published: July 2019

AbstractRangelands are governed by threshold dynamics, and factors such as drought, wildfire, and herbivory can drive change across thresholds and between ecological states. Most work on this topic has focused on shifts in a single response variable, vegetation, and little research has considered how to reconcile responses of more than one variable to determine whether a system has undergone a genuine state change. In sub‐Saharan Africa, mobile overnight livestock corrals (bomas) can be used by managers to precipitate ecological transitions from areas dominated by bare ground to productive ecosystem hotspots (glades) that are attractive to wild herbivores. We asked how long bomas must be occupied by cattle before undergoing a state change, considering both plant and animal response variables, to glade ecosystem hotspots. We tested five durations of boma occupation: 0, 4, 7, 14, and 28 days. Each treatment was replicated five times, and we assessed vegetation as well as herbivore dung (as a proxy of use) at multiple time points over 3 yr following boma abandonment. Vegetation in 7‐, 14‐, and 28‐d boma duration treatments appeared to undergo a complete transition to glade‐like plant communities, whereas the shortest 4‐d treatment had not converted to a glade plant community by year 3. Wildlife responses appeared to lag behind vegetation responses, with transitions to glade‐like herbivore use occurring only in the longest duration (14‐ and 28‐d) treatments. Our results show that different response variables, when considered individually, may provide incomplete or misleading information about state changes. Although shorter‐occupied bomas might be effective for reducing bare ground, they may not attract enough wild herbivores to constitute crossing into an alternative state. Understanding threshold dynamics associated not only with vegetation responses but with a broader suite of response variables is challenging, but will provide a more complete representation of ecosystem function and greater opportunity for more successful ecosystem management.
Mapping Ecosystem Services to Human Well‐being: a toolkit to support integrated landscape management for the SDGs
Authors: Johnson Justin A. , Jones Sarah K. , Wood Sylvia L. R. , Chaplin‐Kramer Rebecca , Hawthorne Peter L. , Mulligan Mark , Pennington Derric , DeClerck Fabrice A. 
Published: July 2019

AbstractThe Sustainable Development Goals (SDGs) emphasize the global and multi‐dimensional nature of sustainability and thus require improving our capacity to articulate and trace the impact of ecosystem change to measures of human well‐being. Yet, the integrated nature of these goals is challenging to assess without similarly integrated assessment tools. We present a new modeling toolkit, “Mapping Ecosystem Services to Human well‐being” (MESH), that integrates commonly used, stand‐alone ecosystem services (ES) models from the InVEST suite of models to quantify and illustrate the trade‐offs and synergies across five ecosystem services and up to 10 associated SDGs. Development of the software and its functionality were informed by a broad stakeholder consultation with ministries, non‐governmental organizations and civil society groups in West Africa to identify common barriers to uptake and application of modeling tools in developing countries. In light of this process, key features included in MESH are (1) integration of multiple ecosystem service (ES) models into a common modeling framework supported by a curated base data set, (2) built‐in scenario generation capacity to support policy analysis, (3) visualization of outcomes and trade‐offs, and (4) mapping of ecosystem service change to SDG targets and goals. We illustrate the use of MESH in a case study in the Volta basin of West Africa comparing the effectiveness of three alternative conservation prioritization approaches: (1) land cover‐based, (2) topographic‐based, and (3) an ecosystem service‐based approach to minimize the impact of agricultural expansion. We evaluate these approaches by linking changes in service supply to potential impacts on achievement of specific SDG goals and targets.
Estimating below‐canopy light regimes using airborne laser scanning: An application to plant community analysis
Authors: Zellweger Florian , Baltensweiler Andri , Schleppi Patrick , Huber Markus , Küchler Meinrad , Ginzler Christian , Jonas Tobias 
Published: July 2019

AbstractLight is a key driver of forest biodiversity and functioning. Light regimes beneath tree canopies are mainly driven by the solar angle, topography, and vegetation structure, whose three‐dimensional complexity creates heterogeneous light conditions that are challenging to quantify, especially across large areas. Remotely sensed canopy structure data from airborne laser scanning (ALS) provide outstanding opportunities for advancement in this respect. We used ALS point clouds and a digital terrain model to produce hemispherical photographs from which we derived indices of nondirectional diffuse skylight and direct sunlight reaching the understory. We validated our approach by comparing the performance of these indices, as well as canopy closure (CCl) and canopy cover (CCo), for explaining the light conditions experienced by forest plant communities, as indicated by the Landolt indicator values for light (Llight) from 43 vegetation surveys along an elevational gradient. We applied variation partitioning to analyze how the independent and joint statistical effects of light, macroclimate, and soil on the spatial variation in plant species composition (i.e., turnover, Simpson dissimilarity, βSIM) depend on light approximation methodology. Diffuse light explained Llight best, followed by direct light, CCl and CCo (R2 = .31, .23, .22, and .22, respectively). The combination of diffuse and direct light improved the model performance for βSIM compared with CCl and CCo (R2 = .30, .27 and .24, respectively). The independent effect of macroclimate on βSIM dropped from an R2 of .15 to .10 when diffuse light and direct light were included. The ALS methods presented here outperform conventional approximations of below‐canopy light conditions, which can now efficiently be quantified along entire horizontal and vertical forest gradients, even in topographically complex environments such as mountains. The effect of macroclimate on forest plant communities is prone to be overestimated if local light regimes and associated microclimates are not accurately accounted for.
Petrogenesis of the Cenozoic Lianhuashan pluton (SW China): Constrained by zircon U–Pb geochronology, Lu–Hf isotope, and geochemistry
Authors: Du Bin , Wang Changming , Yang Lifei , Shi Kangxing , Chen Qi , Zhu Jiaxuan 
Published: July 2019

Potassic magmatic rocks provide significant insights in understanding of mantle composition, evolution, and interaction with crust. Potassic magmatism along the Jinshajiang–Ailaoshan Paleo‐Tethyan suture has drawn much attention. Whereas igneous intrusion in the Simao terrane, west to Jinshajiang–Ailaoshan potassic magmatic belt has rarely been investigated. Hence, we present zircon U–Pb ages and Lu–Hf isotope and whole‐rock geochemistry for the Lianhuashan pluton. Zircon U–Pb dating from monzonite and quartz monzonite porphyry yield ages of 33.6 ± 0.2 and 33.5 ± 0.3 Ma, respectively. Monzonites are characterized by high K2O (4.93–5.16%) and K2O/Na2O (1.46–1.71), high Y (22.90–23.80 ppm), and Yb (1.87–2.21 ppm), relatively enrichment of light rare earth element (LREE). Zircons from monzonites show εHf(t) values ranging from −1.1 to +0.6 and crustal model ages (TDMC) between 1.04 and 1.15 Ga. These geochemical features suggest that parent magmas of the rocks originated from a metasomatized lithospheric mantle. Quartz monzonite porphyries are characterized by high Sr (836.00–1,529.00 ppm), Sr/Y (70.85–99.93) and La/Yb (32.31–49.83), low Y (11.80–15.60 ppm), and Yb (0.95–1.30 ppm), enrichment in LREE and depletion in heavy rare earth elements (HREE). Quartz monzonite porphyries have higher Mg#, MgO, Ni, and Cr contents than the lower crust‐derived adakite‐like rocks. It is suggested that they were derived by variable degree of mixing between lower‐crustal melts and mafic magmas. Based on petrology and geochemistry features, the following evolution process are concluded. During the Neoproterozoic, metasomatic‐enriched mantle magma resulted from the bisubduction of oceanic plate; meanwhile, the crust thickened, and juvenile lower crust formed. During late Eocene, hot asthenosphere upwelled and K‐rich mafic magmas resulted from the decompression of delamination, followed by magmatism emplacing, crystallizing, and the forming of the Lianhuashan pluton.
Clinical characteristics in patients with cervicogenic dizziness: A systematic review
Authors: Knapstad Mari Kalland , Nordahl Stein Helge Glad , Goplen Frederik Kragerud 
Published: July 2019

AbstractBackground and aimsCervicogenic dizziness (CD) is a clinical syndrome of dizziness associated with neck dysfunction. CD represents a considerable diagnostic challenge since dizziness and neck pain are common symptoms with complex and multifactorial etiologies. Both research and clinical work on CD is limited by the lack of accepted diagnostic criteria. The aim of this study was to review clinical studies on CD and to assess current evidence regarding the clinical characteristics of this syndrome.MethodsA comprehensive PubMed and MEDLINE search was conducted from the date of inception of the database, with the last search conducted in September 2018. Included studies had to contain operable diagnostic criteria as well as a comparison between patients considered to have CD and a clinical comparison group. Data extracted were clinical outcomes, diagnostic criteria, age, sex, and sample size. Studies were assessed for methodological quality using the Crowe Critical Appraisal Tool.ResultsOut of 2161 screened studies, eight studies comprising 225 patients met the inclusion criteria. Studies were of low to acceptable methodological quality. The most frequent and consistent clinical characteristic in patients classified as having CD, compared with other populations, was reduced posturographic stability. The most consistent diagnostic criteria were based on the concurrence of neck pain with dizziness after exclusion of other possible reasons for dizziness.ConclusionThere are few studies examining clinical characteristics in patients with cervicogenic dizziness. Altered posturography appeared to be the only consistent characteristic used when distinguishing CD from other populations. Diagnostic criteria currently used in research are likely to have low specificity, since they rest on the exclusion of other causes rather than on positive distinctive features. More studies are needed to better understand the clinical interrelations between dizziness and neck pain.
Catchment chemostasis revisited: Water quality responds differently to variations in weather and climate
Authors: Godsey Sarah E. , Hartmann Jens , Kirchner James W. 
Published: July 2019

AbstractSolute concentrations in streamflow typically vary systematically with stream discharge, and the resulting concentration–discharge relationships are important signatures of catchment biogeochemical processes. Solutes derived from mineral weathering often exhibit decreasing concentrations with increasing flows, suggesting dilution of a kinetically limited weathering flux by a variable flux of water. However, previous work showed that concentration–discharge relationships of weathering‐derived solutes in 59 headwater catchments were much weaker than this simple dilution model would predict. Instead, catchments behaved as chemostats, with rates of solute production and/or mobilization that were nearly proportional to water fluxes, on both event and interannual timescales. Here, we re‐examine these findings using data for a wider range of solutes from 2,186 catchments, ranging from ~10 to >1,000,000 km2 in drainage area and spanning a wide range of lithologic and climatic settings. Concentration–discharge relationships among this much larger set of larger catchments are broadly consistent with the previously described chemostatic behaviour, at least on event and interannual timescales for weathering‐derived solutes. Among these same catchments, however, site‐to‐site variations in mean concentrations of weathering‐derived solutes exhibit strong negative correlations with long‐term average precipitation and discharge, reflecting strong climatic control on long‐term leaching of the critical zone. We use multiple regression of site characteristics including discharge to identify potential controls on long‐term mean concentrations and find that lithologic and land cover controls are significant predictors for many analytes. The picture that emerges is one in which, on event and interannual timescales, weathering‐derived stream solute concentrations are chemostatically buffered by groundwater storage and fast chemical reactions, but each catchment's chemostatic “set point” reflects site‐to‐site differences in climatically driven evolution of the critical zone. In contrast to these weathering products, some nutrients and particulates are often near‐chemostatic across all timescales, and their long‐term mean concentrations correlate more strongly with land use than climatic characteristics.
Evidence for simulation in pharmacy education
Authors: Seybert Amy L. , Smithburger Pamela L. , Benedict Neal J. , Kobulinsky Lawrence R. , Kane‐Gill Sandra L. , Coons James C. 
Published: July 2019

AbstractInnovations in clinical pharmacy education have advanced patient‐centered care knowledge in skills of pharmacy graduates through the use of simulation, virtual reality, and gaming. Pharmacy schools and colleges, as well as health care institutions, have adopted simulation education in various clinical education formats for all levels of learners and practitioners. Over the past 15 years, the University of Pittsburgh School of Pharmacy has embraced innovative approaches to learning and documented advancement of clinical pharmacy education. This brief report will discuss advances in national and international pharmacy education, as well as the PittPharmacy experience with simulation education. A particular emphasis is on human patient simulation, computer‐based simulation, virtual patient, and gaming in the settings of direct patient care‐related education and assessment. The available literature and the experience at PittPharmacy shows improvement in learner satisfaction, knowledge enhancement, critical thinking skills, and problem solving in patient care topics. The evidence included provides support for the use of simulation to advance clinical pharmacy education and can serve to encourage further scholarship in this area.
Ameliorative role of aspirin in paraquat‐induced lung toxicity via mitochondrial mechanisms
Authors: Marashi Sayed Mahdi , Hosseini Seyede Fatemeh , Hosseinzadeh Massood , Qadir Muhammad Farhan , Khodaei Forouzan 
Published: July 2019

AbstractParaquat (PQ) has accounted for numerous suicide attempts in developing countries. Aspirin (ASA) as an adjuvant treatment in PQ poisoning has an ameliorative role. And, it's uncoupling of mitochondrial oxidative phosphorylation role has been well established. The current study aimed at examining the aspirin mechanism on lung mitochondria of rats exposed to PQ. Male rats were randomly allocated in five groups: Control group, PQ group (50 mg/kg; orally, only on the first day), and PQ + ASA (100, 200, and 400 mg/kg; i.p.) groups for 3 weeks. Mitochondrial indices and respiratory chain‐complex activities were determined. PQ induced lung interstitial fibrosis; however, ASA (400 mg/kg) led to decrease in this abnormal alteration. In comparison with PQ group, complex II and IV activity, and adenosine triphosphate content in ASA groups had significantly increased; however, reactive oxygen species production, mitochondrial membrane permeabilization, and mitochondrial swelling were significantly reduced. In conclusion, aspirin can alleviate lung injury induced by PQ poisoning by improving mitochondrial dynamics.