bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal and neonatal outcome of births in alongside midwifery units: A cohort study from a tertiary center in Germany
Authors: Merz Waltraut Maria , Tascon-Padron Laura , Puth Marie-Therese , Heep Andrea , Tietjen Sophia L. , Schmid Matthias , Gembruch Ulrich 
Published: September 2019

Abstract
Background For healthy women entering birth after uneventful pregnancy, midwife-led models of care have the potential to reduce interventions and increase the vaginal birth rate without compromising the maternal or health of the neonate. In Germany, 98.4% of women are giving birth in consultant-led obstetric units. Alongside midwifery units (AMU) have been established in 2003. We compared the outcome of all births in the AMU at our hospital from 2010 to 2017 with a matched group of low-risk women who gave birth during the same period of time in standard obstetric care. Methods We used a retrospective cohort study design. The study group consists of all women admitted to labor ward who had registered for birth in AMU. For the control group, low-risk women were selected; additionally, matching was performed for parity. Mode of birth, postpartum hemorrhage, and obstetric injury was chosen as primary outcome parameter for the mother. For the neonate, a composite primary outcome (5-minute Apgar <7 or umbilical cord arterial pH < 7.20 or transfer to specialist neonatal care) was defined. Secondary outcomes included epidural analgesia, duration of the second stage of labor, and episiotomy rate. Non-inferiority was assessed, and multiple logistic regression analysis was performed. Results 612 women were admitted for labor in AMU, the control group consisted of 612 women giving birth in standard obstetric care. Women in the study group were on average older and had a higher BMI; birthweight was on average 95 g higher. Except for birth injuries, non-inferiority could be established for the primary outcomes. Secondary outcomes occurred less common in the study group, including a shorter duration of the second stage of labor. Overall, 50.3% of women were transferred to standard obstetric care. Regression analysis revealed effects of parity, age and birthweight on the chance of transfer. Conclusion Our investigation confirms comparable maternal and neonatal outcome with less interventions for women giving birth in AMU at our institution. Currently, obstetric services in Germany are almost exclusively provided by consultant-led units. Our results support the integration of AMU as complementary models of care for low-risk women.
Trends in the consumption rates of benzodiazepines and benzodiazepine-related drugs in the Health Region of Lleida from 2002 to 2015
Authors: Torres-Bondia Francisco , De Batlle Jordi , Galvan Leonardo , Buti Miquel , Barbé-Illa Ferran , Piñol Gerard 
Published: September 2019

Abstract
Background: To determine the trend in the consumption prevalence of benzodiazepines (BZDs) and drugs related to BZDs (Z-drugs) in the population of the Health Region of Lleida to explore patterns of use and characteristics associated between 2002 and 2015. Methods: Retrospective community cohort study carried out between 2002 and 2015 consisting of individuals from a Health Region of 358,157 inhabitants in 2015. The consumption of BZDs was evaluated using prescription billing data from the Public Health System . All types of BZDs and their analogues that have been approved by the drug agency were included. Trends regarding age and sex were investigated. Results: A total of 249,471 individuals generated 338,148 dispensations. Overall, 53% were women, and the mean age was 60 years. The prevalence of BZD use in 2015 was 14.2%—18.8% in women and 9.6% in men—and was 36% in those over 65 years. According to the half-life of BZDs, the prevalence of short-intermediate BZD use was 9.7%, 5.5% for intermediate-long BZDs, and 0.8% for Z-drugs. The evolution in consumption showed a progressive decline, from 15.3% in 2002 to 14.2% in 2015, which is attributed to a decrease in consumption of intermediate-long BZDs (8.0% vs. 5.5%) and Z-drugs (1.4% vs. 0.8%). Conclusion: The prescription prevalence of BZDs and Z-drugs was high, although a small reduction was observed during this time period. The population over 65 was the most prevalent in their consumption despite cognitive risk and falls. Integral actions are required to lower their prescription rate.
Genome-wide identification and analysis of WRKY gene family in jute (Corchorus capsularis)
Authors: Zhang Lilan , Wan Xuebei , Xu Yi , Niyitanga Sylvain , Qi Jianmin , Zhang Liwu 
Published: September 2019

Abstract
Background WRKY transcription factor is a kind of transcription factor which plays an important role in plant response to biotic, abiotic stress, plant growth and development. However, little information was available about the WRKY genes in jute ( Corchorus capsularis ).Results In the present study, 43 jute WRKY (CcWRKY) genes were identified by using Pfam database domain search and BLAST homology alignment based on the transcriptome data of jute. And the gene structure, phylogeny, conserved domain and three-dimensional structure of protein were also analyzed by GSDS2.0, MEGA7.0, DNAMAN5.0, WebLogo 3 and SWISS-MODEL bioinformatics tools. According to the WRKY conserved domain features and the evolution analysis with Arabidopsis thaliana , 43 members were divided into three classes: I, II and III containing 9, 28, 6 members, respectively. According to the evolutionary relationship, class II further divided into five subclasses: II-a (2), II-b (7), II-c (7), II-d (6) and II-e (6). Genetic structure analysis showed that exon and intron number of CcWRKY genes had high variability (3-11 exons), even within the same subgroup. Most of the CcWRKY genes were expressed in different tissues, but they were mainly expressed in stem bark and stem stick. After GA 3 stress, the expression of most WRKY genes in GA 3 -sensitive variety "Aidianyehuangma" was significantly different from that of normal variety "Huangma 179". These results indicated that CcWRKY genes play an important role in gibberellin biosynthesis pathway and fiber development.Conclusions CcWRKY proteins are highly conserved, the length of the gene sequence and the number of introns varied widely, all WRKY genes showed a variety of expression patterns in different tissues, most of the WRKY genes responded to GA 3 stress, which play an important role in gibberellin biosynthesis pathway and bast fiber development.
Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cells
Authors: Taylor Alison , Rudd Christopher E. 
Published: September 2019

Abstract
Objective: The threonine/serine kinase glycogen synthase kinase 3 (GSK-3) targets multiple substrates in T-cells, regulating the expression of Tbet and PD-1 on T-cells. However, it has been unclear whether GSK-3 can affect the motility of T-cells and their interactions with antigen presenting cells.
Results: Here, we show that GSK-3 can regulate T-cell motility. Inhibition of GSK-3, by small molecule SB415286, reduced T-cell motility in terms of distance and displacement. Although SB415286 reduced the number of contacts, the dwell times of established contacts did not differ between T-cells treated with SB415286. These data indicate show that the inhibition of GSK-3 can influence the motility of T-cells and interactions with other cells without affecting the dwell times of cells that make productive contacts.
Hepatitis B and C Virus, Human Immunodeficiency Virus Co-infection among Pregnant Women in Semi-rural Health District, Cameroon
Authors: NGOUNOUH christian TAHEU , Taheu Christian Ngounouh , Nguwoh Philippe Salomon , Bazu Armelle Diane Pouanse , Ewoele Esther Celeste , Egoume Laure Genévieve , Mbende Jean Otélé , Assam Jean Paul Assam , Assoumou Marie Claire Okomo , Moyou Roger Somo , Fokam Joseph 
Published: September 2019

Abstract
Background In Cameroon, Human Immunodeficiency Virus (HIV), Hepatitis B (HBV) and C virus (HCV) are highly endemic to the pregnant woman. These viruses pose a high risk of vertical transmission and have been reported as the most important causes of maternal mortality. The aim objective of this study was to determine the hepatitis B, C and HIV virus co-infection among pregnant women in the Bafia health district.Methods A cross-sectional study was conducted from may to july 2018 in 145 pregnant women attending the health district of Bafia. HIV was diagnosed and confirmed using the Determine and Oraquick HIV1/2, HBV by NOVA test (HBV Multi Panel One Step) and HCV by ACCURATE test. Statistical analysis was performed using the Epi info software version 7.2.1.0. Furthermore, statistical association was performed using Odds Ratio (OR) and Fisher Exact test where appropriate, with corresponding 95% confidence interval (CI). The probability was considered statistical significant for all values p<0.05.Results The average age was 25 ± 5.96 years and the seroprevalence of HIV, HBV and HCV was 6.90% (10/145), 10.34% (15/145) and 33.79% (49/145), respectively. Those aged 24-32 years had peaks of infection, with 9.84% (6/61) and 45.90% (28/61) of HIV, Odds Ratio (OR): 2.18 [95%CI: 0.58-8.09], p= 0.39 and HCV, OR: 2.54 [95%CI: 1.25-5.15], p= 0.008 respectively. However, the peak of HBV (HBsAg) infection (35.29% [6/17]) was in the age group 33-41 years, p= 0.001 and carriage rate of HBeAg was only observed in those aged 15-23 years (5.97% [4/67], p=0.30). No cases of co-infection were observed between HBsAg carrier rates (p= 0.56), HBeAg (p= 0.65) and HIV. Meanwhile, 10.20% (5/49) of pregnant women were co-infected with HCV/HIV, OR: 2.06 [95%CI: 0.56-7.51], p= 0.26 and this co-infection was observed among those aged 24-32 years (8.20% [5/61], p= 0.02).Conclusion Seroprevalence of HIV, HBV and HCV in pregnant women remain high in the Bafia health district, reflecting a significant risk of vertical transmission, especially HIV/HCV co-infection. The prevention strategies of these three viruses would primarily target pregnant women aged over 24 years in health care areas with a typology similar to that of Bafia.
Effect of periodontal therapy on the systemic inflammation and metabolic markers in patients undergoing hemodialysis or/and peritoneal dialysis: a systematic review and meta-analysis
Authors: Yue Hui , Xu Xinxin , Liu Qin , Li Xiaozhi , Xiao Yiting , Hu Bo 
Published: September 2019

Abstract
Background: This systematic review aimed to investigate whether periodontal treatment can reduce the systemic inflammatory levels and improve the metabolic levels in patients undergoing hemodialysis (HD) or/and peritoneal dialysis (PD).
Methods: Electronic databases (PubMed, EMBASE, CENTRAL, NCKI, and WFPD) were searched up to July 2019. The risk of bias within studies was assessed through the Cochrane Collaboration' s risk assessment tool. The systemic inflammatory and metabolic measures were the highly sensitive C-reactive protein (hs-CRP), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-a), the albumin (Alb), and lipid metabolic levels. Meta-analyses (MAs) were performed to calculate the overall effect size where appropriate.
Results: Five studies were eligible for this systematic review. The result of four studies revealed a significant difference in the CRP level after periodontal treatment in patients receiving HD or/and PD. Two studies reported the IL-6 and the Alb level after periodontal treatment but revealed no significant difference. No MAs could be performed on the TNF- a level and the lipid metabolic markers.
Conclusions: Periodontal treatment may moderately reduce the serum of CRP levels in HD or/and PD patients. For the TNF-a, IL-6, Alb levels and lipid metabolic markers, no sufficient evidence supports the difference after periodontal treatment. Therefore, larger scales and high-quality randomized-controlled trials (RCTs) are required to assess the effect of periodontal treatment on systemic inflammatory and metabolic parameters in HD or/and PD patients.
Overexpression of miR-222-3p promotes proliferation and inhibits apoptosis in diffuse large B-cell lymphoma cells via suppressing PPP2R2A
Authors: Sun Shanshan , Wang Hui , Ji Mingyou 
Published: September 2019

Abstract
Background This study aimed to investigate the mechanism of microRNA-222-3p (miR-222-3p) on the progression of diffuse large B-cell lymphoma (DLBCL) cells.Methods DLBCL tissue was isolated from DLBCL patients during surgery. OCI-LY10 and U2932 cells were cultured. Then, qRT-PCR, Western blot, luciferase reporter gene assay, RNA pull-down assay, MTT assay, colony formation analysis, flow cytometry as well as Transwell assay were used to observe the effect of miR-222-3p on proliferation, migration, invasion and apoptosis of DLBCL cells. Furthermore, the tumor growth affected by miR-222-3p was further investigated based on animal experiment.Results Compared with the control group, the expression level of miR-222-3p was up-regulated in DLBCL group. The luciferase reporter gene and RNA pull down assay showed that PPP2R2A 3’-untranslated region (3’-UTR) carried the directly binding site of miR-222-3p. Furthermore, MTT assay, colony formation, qRT-PCR and Western blot showed that miR-222-3p promoted the DLBCL cell proliferation and invasion, and inhibited apoptosis. Finally, the mice experiment showed that miR-222-3p mimics inhibited PPP2R2A expression and promoted tumor growth in vivo.Conclusions Upregulation of miR-222-3p might take part in the progression of DLBCL by suppressing PPP2R2A expression. Furthermore, miR-222-3p promoted the DLBCL cell proliferation and invasion, and inhibited apoptosis.
The early diagnostic value of combined detection of plasma miR-181b, miR-196a and miR-210 in pancreatic cancer
Authors: Liu Gongpan , Shao Cunhua , Li Anyun , Zhang Xiaobin , Guo Xingjun , Li Jiangong 
Published: September 2019

Abstract
Background This study aimed to investigate the effect of combination of plasma miR-181b, miR-196a and miR-210 on early diagnosis of pancreatic cancer (PC).Methods In our study, the plasma was isolated from patients with PC and healthy individuals, respectively. The expressions of miR-181b, miR-196a and miR-210 were detected by qRT-PCR. Moreover, the level of carbohydrate antigen 199 (CA199) was measured by electrochemiluminescence (ECL) assay. Furthermore, the area under the receiver operating characteristic (ROC) curve (AUC) was used to analyze the diagnostic efficacy of miR-181b, miR-196a, miR-210 and CA199, as well as the combination of thses miRNAs in early PC patients and healthy individuals.Results The expressions of miR-181b, miR-196a and miR-210 were significantly upregulated in PC patients. In addition, the level of CA199 was also significantly upregulated in the plasma of PC patients. The expressions of miR-181b, miR-196a and miR-210 were closely associated with lymph nodes metastasis, clinical stage and vascular invasion, but had no correlation with the patient's age, gender and tumor size. Moreover, miR-181b, miR-196a and miR-210 have lower AUC than CA199 in PC patients. The combinations miR-181b + miR-196a, miR-181b + miR-210, miR-196a + miR-210 also have lower AUC than CA199 in PC patients. It is worth noting that the combinations miR-181b + miR-196a + miR-210 have higher AUC than CA199 in PC.Conclusions Our study demonstrated that the combination of plasma miR-181b, miR-196a and miR-210 had good value for PC early diagnosis.
Long noncoding RNA UCA1 facilitates cell proliferation and inhibits apoptosis via activating PI3K/Akt pathway in retinoblastoma
Authors: Yuan Zhongfang , Li Zhaona 
Published: September 2019

Abstract
Background This study aimed to explored the effect of lncRNA-UCA1 on retinoblastoma (RB) and its potential molecular mechanisms.Methods In our study, the expression of lncRNA-UCA1 was measured by qRT-RCR in both RB tissues and RB HXO-RB44 and Y79 cells. The relationship between lncRNA-UCA1 expression and clinical parameters in RB patients were evaluated. Cell proliferation, cell clones, apoptosis and cell cycle of HXO-RB44 and Y79 cells were measured by cell counting kit-8 (CCK-8) assay, colony formation assay and flow cytometry, respectively. In addition, the expressions of PCNA, Caspase-3, survivin, p16, p21, CDK2, PI3K, p-PI3K, Akt, p-Akt and S6k in HXO-RB44 and Y79 cells were measured by western blot.Results lncRNA-UCA1 was highly expressed in both RB tissues and RB HXO-RB44 and Y79 cells. Moreover, the expression of lncRNA-UCA1 in RB patients was remarkedly correlated with tumor size, optic nerve invasion and pathologic grade. lncRNA-UCA1 markedly facilitated cell proliferation and cell cycle procession, as well as inhibited cell apoptosis in HXO-RB44 and Y79 cells. lncRNA-UCA1 dramatically increased the expression of S6k and the phosphorylation of PI3K and Akt in RB cells. LY294002 (PI3K inhibitor) reversed the effects of lncRNA-UCA1 on RB cell proliferation, apoptosis and cell cycle procession.Conclusions Our study indicated that lncRNA-UCA1 could promote cell proliferation and cell cycle procession, as well as inhibit cell apoptosis in RB via activating PI3K/Akt pathway.
Functional polymorphisms in the MED13L and SLC4A7 genes are associated with pregnancy-induced hypertension
Authors: Zhou Ying , Li Xun , Hu Shimin , Zhou Shujin , Tan Hongzhuan 
Published: September 2019

Abstract
Background: Pregnancy-induced hypertension (PIH), including gestational hypertension (GH) and preeclampsia (PE), might share pathophysiologic mechanisms and genetic risk factors with hypertension. This study aimed to investigate whether genetic risk factors for hypertension also been associated with PIH.
Methods: This is a case control candidate gene association study. A total of 84 cases with GH, 108 cases with PE, and 192 age-matched (±1 year) controls were recruited from the Liuyang Municipal Hospital of Maternal and Child Health, Hunan Province of China. Three candidate single nucleotide polymorphisms (SNPs) which have been found to be associated with hypertension or blood pressure (BP) were chosen. The associations between SNPs and GH and PE were analyzed by multiple logistic regression models after adjusting for fetal sex and primipara.
Results: GG genotype at mediator complex subunit 13-like (MED13L) rs11067763 was a protective factor for developing GH (GG vs. AA + AG, OR = 0.376, 95%CI = 0.158-0.892) and PE (GG vs. AA + AG, OR = 0.489, 95%CI = 0.245-0.979) in recessive model. AA genotype at solute carrier family 4 member 7 (SLC4A7) rs820430 was a risk factor for developing GH (AA vs. GG + AG, OR = 3.562, 95%CI = 1.343-9.447) and PE (AA vs. GG + AG, OR = 3.351, 95%CI = 1.352-8.303) in recessive model. But no significant association was observed between cytochrome P450 family 21 subfamily A member 2 (CYP21A2) rs2021783 and the GH/PE groups.
Conclusions: PIH and hypertension are likely to share genetic risk factors. The associations both between variants of MED13L rs11067763 and GH/PE and between variants of SLC4A7 rs820430 and GH/PE were significant.
Network analyses of circular RNAs expression profiles and their hub genes in pancreatic ductal adenocarcinoma
Authors: Tang Yanyan , Zhang Ping 
Published: September 2019

Abstract
Pancreatic ductal adenocarcinoma (PDAC) is one of the most common malignant tumor in digestive system. CircRNAs involve in lots of biological processes through interacting with miRNAs and their targeted mRNA. We obtained the circRNA gene expression profiles from Gene Expression Omnibus (GEO) and identified differentially expressed genes (DEGs) between PDAC samples and paracancerous tissues. Bioinformatics analyses, including GO analysis, KEGG pathway analysis and PPI network analysis, were conducted for further investigation. We also constructed circRNA‑microRNA-mRNA co-expression network. A total 291 differentially expressed circRNAs were screened out. The GO enrichment analysis revealed that up-regulated DEGs were mainly involved metabolic process, biological regulation, and gene expression, and down-regulated DEGs were involved in cell communication, single-organism process, and signal transduction. The KEGG pathway analysis, the upregulated circRNAs were enriched cGMP-PKG signaling pathway, and HTLV-I infection, while the downregulated circRNAs were enriched in protein processing in endoplasmic reticulum, insulin signaling pathway, regulation of actin cytoskeleton, etc. Four genes were identified from PPI network as both hub genes and module genes, and their circRNA‑miRNA-mRNA regulatory network also be constructed. Our study indicated possible involvement of dysregulated circRNAs in the development of PDAC and promoted our understanding of the underlying molecular mechanisms.
Clinical and prognostic differences between methicillin-resistant and methicillin-susceptible Staphylococcus aureus infective endocarditis
Authors: Tenorio Carmen Hidalgo , Gálvez Juan , Martínez-Marcos Francisco Javier , Plata-Ciezar Antonio , Torre-Lima Javier De La , López-Cortés Luis Eduardo , Noureddine Mariam , Reguera José M. , Vinuesa David , García Maria Victoria , Ojeda Guillermo , Luque Rafael , Lomas José Manuel , Lepe Jose Antonio , de Alarcón Arístides 
Published: September 2019

Abstract
Background: S. aureus(SA) infective endocarditis (IE)has a very high mortality, attributed to the age and comorbidities of patients, inadequate or delayed antibiotic treatment, and methicillin resistance, among other causes. The main study objective was to analyze epidemiological and clinical differences between IE by methicillin-resistantversusmethicillin-susceptible SA (MRSA vs. MSSA)and to examine prognostic factors for SA endocarditis, including methicillin resistance and vancomycin minimum inhibitory concentration (MIC) values >1 ug/mL to MRSA. Methods: Patientswith SA endocarditis were consecutively and prospectively recruited from the Andalusia endocarditis cohort between 1984 and January 2017. Results: We studied 437 patients with SA endocarditis, which was MRSA in 13.5% of cases. A greater likelihood of history of COPD (OR 3.19; 95% CI 1.41-7.23), invasive procedures, or recognized infection focus in the three months before IE onset (OR 2.9; 95% CI 1.14-7.65) and of diagnostic delay (OR 3.94; 95% CI 1.64-9.5) was observed in patients with MRSA versusMSSA endocarditis. The one-year mortality rate due to SA endocarditis was 44.3% and associated with decade of endocarditis onset (1985-1999) (OR 8.391; 95% CI (2.82-24.9); 2000-2009 (OR 6.4; 95% CI 2.92-14.06); active neoplasm (OR 6.63; 95% CI 1.7-25.5) and sepsis (OR 2.28; 95% CI 1.053-4.9). Methicillin resistance was not associated with higher IE-related mortality (49.7 vs. 43.1%; p= 0.32). Conclusion: MRSA IE is associated with COPD, previous invasive procedure or recognized infection focus, and nosocomial or healthcare-related origin.Methicillin resistance does not appear to be a decisive prognostic factor for SA IE.