bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE ROLES OF INSTITUTIONAL INVESTORS IN THE FAILURE OF NEWLY PUBLIC STOCKS
Authors: Qian Hong , Ramalingegowda Santhosh , Zhong Zhaodong (Ken) 
Published: August 2019

AbstractIn this article, we study two negative events that can happen to newly public stocks: (1) the price drops at least 50% from the closing price on the first trading date within one year after the initial public offering (IPO) (initial failure) and (2) the firm is delisted for negative reasons within three years after the IPO (final failure). We find that high investor sentiment at the time of IPO can lead to both initial failure and final failure of IPO firms, whereas monitoring by external professionals plays a more important role in averting final failure than initial failure. Exploring the roles of different types of institutional investors, we find that transient (i.e., short‐term trading) institutions sell before initial failure. In contrast, dedicated (i.e., monitoring) institutions focus on long‐term performance and may stay with stocks suffering temporary initial failure, but their selling typically signals the imminent final failure of newly public firms.
New insights into the development of the human cerebral cortex
Authors: Molnár Zoltán , Clowry Gavin J. , Šestan Nenad , Alzu'bi Ayman , Bakken Trygve , Hevner Robert F. , Hüppi Petra S. , Kostović Ivica , Rakic Pasko , Anton E. S. , Edwards David , Garcez Patricia , Hoerder‐Suabedissen Anna , Kriegstein Arnold 
Published: August 2019

AbstractThe cerebral cortex constitutes more than half the volume of the human brain and is presumed to be responsible for the neuronal computations underlying complex phenomena, such as perception, thought, language, attention, episodic memory and voluntary movement. Rodent models are extremely valuable for the investigation of brain development, but cannot provide insight into aspects that are unique or highly derived in humans. Many human psychiatric and neurological conditions have developmental origins but cannot be studied adequately in animal models. The human cerebral cortex has some unique genetic, molecular, cellular and anatomical features, which need to be further explored. The Anatomical Society devoted its summer meeting to the topic of Human Brain Development in June 2018 to tackle these important issues. The meeting was organized by Gavin Clowry (Newcastle University) and Zoltán Molnár (University of Oxford), and held at St John's College, Oxford. The participants provided a broad overview of the structure of the human brain in the context of scaling relationships across the brains of mammals, conserved principles and recent changes in the human lineage. Speakers considered how neuronal progenitors diversified in human to generate an increasing variety of cortical neurons. The formation of the earliest cortical circuits of the earliest generated neurons in the subplate was discussed together with their involvement in neurodevelopmental pathologies. Gene expression networks and susceptibility genes associated to neurodevelopmental diseases were discussed and compared with the networks that can be identified in organoids developed from induced pluripotent stem cells that recapitulate some aspects of in vivo development. New views were discussed on the specification of glutamatergic pyramidal and γ‐aminobutyric acid (GABA)ergic interneurons. With the advancement of various in vivo imaging methods, the histopathological observations can be now linked to in vivo normal conditions and to various diseases. Our review gives a general evaluation of the exciting new developments in these areas. The human cortex has a much enlarged association cortex with greater interconnectivity of cortical areas with each other and with an expanded thalamus. The human cortex has relative enlargement of the upper layers, enhanced diversity and function of inhibitory interneurons and a highly expanded transient subplate layer during development. Here we highlight recent studies that address how these differences emerge during development focusing on diverse facets of our evolution.
A role for gangliosides and β1‐integrin in the motility of olfactory ensheathing glia
Authors: Santos‐Silva Alessandra , Piña‐Rodrigues Felipe Márquez , Mermelstein Claudia dos Santos , Allodi Silvana , Barradas Penha C. , Cavalcante Leny A. 
Published: August 2019

AbstractOlfactory ensheathing glia (OEG) are found in the olfactory mucosa, nerve and bulb, and provide in vivo ensheathment for the unmyelinated olfactory axons within the central and peripheral nervous system domains. OEG cells are able to migrate long distances within the neuropil of the central nervous system. Because gangliosides such as 9‐O‐acetyl GD3 have crucial regulatory roles in neuronal migration during development, we analyzed whether OEG in organotypical cultures are revealed by anti‐9‐O‐acetyl GD3 and/or gangliosides are recognized by the A2B5 antibody (G‐A2B5), and whether these gangliosides are involved in OEG migration. Our results showed that all OEG migrating out of a section of olfactory bulb onto a laminin substrate bound to the 9‐O‐acetyl GD3 and A2B5 antibodies, and that 2′,3′‐cyclic nucleotide phosphodiesterase (CNPase) colocalized with 9‐O‐acetyl GD3 and with G‐A2B5. Additionally, we showed that the immune blockade of 9‐O‐acetyl GD3 or G‐A2B5 reduced the migration of OEG on laminin, and that 9‐O‐acetyl GD3 and G‐A2B5 colocalized with the β1‐integrin subunit. We also confirmed the phenotype of in‐vitro‐grown OEG cells derived from adult rats, showing that they express CNPase, and also α‐smooth muscle actin, which is not expressed by Schwann cells. Our data showed that the gangliosides 9‐O‐acetyl GD3 and G‐A2B5 participate in the migratory activity of OEG cells, and that the β1‐integrin subunit colocalizes with these gangliosides. These results suggest a new role for β1‐integrin and gangliosides in the polarized migration of OEG cells, and provide new information on the molecules controlling OEG motility and behavior.
Formulation and optimisation of novel transfersomes for sustained release of local anaesthetic
Authors: Bnyan Ruba , Khan Iftikhar , Ehtezazi Touraj , Saleem Imran , Gordon Sarah , O’Neill Francis , Roberts Matthew 
Published: August 2019

Abstract

Objective
To investigate the effect of formulation parameters on the preparation of transfersomes as sustained-release delivery systems for lidocaine and to develop and validate a new high-performance liquid chromatography (HPLC) method for analysis.

Method
Taguchi design of experiment (DOE) was used to optimise lidocaine-loaded transfersomes in terms of phospholipid, edge activator (EA) and phospholipid : EA ratio. Transfersomes were characterised for size, polydispersity index (PDI), charge and entrapment efficiency (%EE). A HPLC method for lidocaine quantification was optimised and validated using a mobile phase of 30%v/v PBS (0.01 m) : 70%v/v Acetonitrile at a flow rate of 1 ml/min, detected at 255 nm with retention time of 2.84 min. The release of lidocaine from selected samples was assessed in vitro.

Key findings
Transfersomes were 200 nm in size, with PDI ~ 0.3. HPLC method was valid for linearity (0.1–2 mg/ml, R2 0.9999), accuracy, intermediate precision and repeatability according to ICH guidelines. The %EE was between 44% and 56% and dependent on the formulation parameters. Taguchi DOE showed the effect of factors was in the rank order : lipid : EA ratio ˃ EA type ˃ lipid type. Optimised transfersomes sustained the release of lidocaine over 24 h.

Conclusion
Sustained-release, lidocaine-loaded transfersomes were successfully formulated and optimised using a DOE approach, and a new HPLC method for lidocaine analysis was developed and validated.
Northern Hemisphere megafossil of Dacrycarpus (Podocarpaceae) from the Miocene of South China and its evolutionary and paleoecological implications
Authors: Wu Xin‐Kai , Zavialova Natalia E. , Kodrul Tatiana M. , Liu Xiao‐Yan , Gordenko Natalia V. , Maslova Natalia P. , Quan Cheng , Jin Jian‐Hua 
Published: August 2019

AbstractThe modern genus Dacrycarpus (Endl.) de Laub. of the family Podocarpaceae, containing nine species, is mainly distributed in tropical mountain rainforests of the southwestern Pacific region, ranging from New Zealand to low‐latitude Asia. This genus has abundant fossil records in both hemispheres, but all the known megafossils were limited to Australasia and South America. Here we report on Dacrycarpus guipingensis sp. nov. from the Miocene Erzitang Formation of Guangxi, South China. This is the first megafossil of Dacrycarpus in the Northern Hemisphere. The new species is represented by mummified dimorphic foliage, ovuliferous shoots, and a male cone with in situ pollen. It resembles the extant Dacrycarpus imbricatus (Blume) de Laub., which is common in rainforests from southern China and northern Myanmar to Fiji. This paper presents the first data on the anatomical structure of seed cone and exine ultrastructure of Dacrycarpus in situ pollen grains from a fossil material by using computed tomography scanning and ultrathin sectioning. For comparative purpose, data on the pollen morphology and ultrastructure were obtained for modern D. imbricatus for the first time. The D. guipingensis fossils strongly suggest the Miocene arrival of Dacrycarpus in Asia from the Southern Hemisphere. Based on the modern ecological niche and related fossil elements, this fossil locality was probably covered by conifer–broad‐leaved mountain rainforests during the Miocene.
Children With Developmental Language Disorder Have an Auditory Verbal Statistical Learning Deficit: Evidence From an Online Measure
Authors: Lammertink Imme , Boersma Paul , Wijnen Frank , Rispens Judith 
Published: August 2019

Successful language use requires the ability to process nonadjacent dependencies (NADs) that occur in linguistic input. Learning such structural regularities seems therefore crucial for children, and researchers have indeed proposed that language problems in children with developmental language disorder (DLD), especially problems with grammar, are due to their decreased sensitivity to NADs. Because the evidence supporting this claim is scarce, we compared children with DLD (n = 36; Mage = 9.1 years) and without DLD (n = 36; Mage = 9.1 years) performing a learning task with NADs. Using response times as an online measure of learning NADs, we observed that participants with DLD were less sensitive to NADs than were typically developing peers. The confidence intervals of the effect, however, indicated that the effect was probably small in size. We discuss clinical and theoretical implications of the present study in light of this effect size.Open PracticesThis article has been awarded Open Materials and Open Data badges. All data, materials, and analysis scripts for this study are publicly accessible via the Open Science Framework at https://osf.io/8a3yv. The study materials are also publicly available via the IRIS database at https://www.iris-database.org. Learn more about the Open Practices badges from the Center for Open Science: https://osf.io/tvyxz/wiki.
Divergent gene expression networks underlie morphological diversity of abscission zones in grasses
Authors: Yu Yunqing , Hu Hao , Doust Andrew N. , Kellogg Elizabeth A. 
Published: August 2019

Summary

Abscission is a process in which plants shed their parts, and is mediated by a particular set of cells, the abscission zone (AZ). In grasses (Poaceae), the position of the AZ differs among species, raising the question of whether its anatomical structure and genetic control are conserved.

The ancestral position of the AZ was reconstructed. A combination of light microscopy, transmission electron microscopy, RNA‐Seq analyses and RNA in situ hybridisation were used to compare three species, two (weedy rice and Brachypodium distachyon) with the AZ in the ancestral position and one (Setaria viridis) with the AZ in a derived position below a cluster of flowers (spikelet).

Rice and Brachypodium are more similar anatomically than Setaria. However, the cell wall properties and the transcriptome of rice and Brachypodium are no more similar to each other than either is to Setaria. The set of genes expressed in the studied tissues is generally conserved across species, but the precise developmental and positional patterns of expression and gene networks are almost entirely different.

Transcriptional regulation of AZ development appears to be extensively rewired among the three species, leading to distinct anatomical and morphological outcomes.
Palaeoclimatic reconstruction for a Cenomanian‐aged angiosperm flora near Schefferville, Labrador
Authors: Demers‐Potvin Alexandre V. , Larsson Hans C. E. 
Published: August 2019

AbstractAn understanding of local and regional climate trends is essential to investigate the remarkable angiosperm radiation that happened during the Albian–Cenomanian transition. However, many of the inland depositional environments pioneered by the first modern angiosperms are poorly represented in the fossil record. Eastern Canada, in particular, has a very poor Mesozoic record. In this paper, we present the first multivariate palaeoclimate analysis (CLAMP) for the environment of a geologically isolated woody dicot dominated flora found in the Redmond no.1 mine, Labrador, near Schefferville, with an estimated Cenomanian age. It reveals that the Redmond flora would have experienced a mean annual temperature of 15.1 ± 2.1°C, one of the coolest recorded for North America at this time. These results confer the Redmond no.1 site a warm temperate and fully humid climate with a hot summer, in accordance with previous qualitative palaeoclimate estimates. This flora fits smoothly into palaeolatitudinal MAT gradients that use other Cenomanian‐estimated North American floras. Despite an inland setting, the climate analysis does not recover a significantly higher degree of seasonality than the sites to which it is compared, which agrees with established climate equability models for the Cretaceous and Palaeogene. This study also introduces 15 new morphotypes discovered in recent fieldwork. The eventual description of the species they represent may refine our dating estimates for the Redmond Formation. A greater understanding of the depositional environment and of the natural history of these angiosperms is required to improve this community's characterization, along with estimates from other proxies.
In‐depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host‐specific post‐translational modifications
Authors: van Eerde André , Várnai Anikó , Jameson John Kristian , Paruch Lisa , Moen Anders , Anonsen Jan Haug , Chylenski Piotr , Steen Hege Særvold , Heldal Inger , Bock Ralph , Eijsink Vincent G. H. , Liu‐Clarke Jihong 
Published: August 2019

SummarySustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass. Plants offer a safe and cost‐effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels. Production of intact and functional protein is a prerequisite for large‐scale protein production, and extensive host‐specific post‐translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes. Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme. TrCel7A was produced in Nicotiana benthamiana using a vacuum‐based transient expression technology, and this recombinant enzyme (TrCel7Arec) was compared with the native fungal enzyme (TrCel7Anat) in terms of PTMs and catalytic activity on commercial and industrial substrates. We show that the N‐terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O‐mannosylation in the plant host as compared with the native protein. In general, the purified full‐length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate‐binding properties, which can be attributed to larger N‐glycans and lack of O‐glycans in TrCel7Arec. All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.
Knockout of two BnaMAX1 homologs by CRISPR/Cas9‐targeted mutagenesis improves plant architecture and increases yield in rapeseed (Brassica napus L.)
Authors: Zheng Ming , Zhang Liang , Tang Min , Liu Jinglin , Liu Hongfang , Yang Hongli , Fan Shihang , Terzaghi William , Wang Hanzhong , Hua Wei 
Published: August 2019

SummaryPlant height and branch number are essential components of rapeseed plant architecture and are directly correlated with its yield. Presently, improvement of plant architecture is a major challenge in rapeseed breeding. In this study, we first verified that the two rapeseed BnaMAX1 genes had redundant functions resembling those of Arabidopsis MAX1, which regulates plant height and axillary bud outgrowth. Therefore, we designed two sgRNAs to edit these BnaMAX1 homologs using the CRISPR/Cas9 system. The T0 plants were edited very efficiently (56.30%–67.38%) at the BnaMAX1 target sites resulting in homozygous, heterozygous, bi‐allelic and chimeric mutations. Transmission tests revealed that the mutations were passed on to the T1 and T2 progeny. We also obtained transgene‐free lines created by the CRISPR/Cas9 editing, and no mutations were detected in potential off‐target sites. Notably, simultaneous knockout of all four BnaMAX1 alleles resulted in semi‐dwarf and increased branching phenotypes with more siliques, contributing to increased yield per plant relative to wild type. Therefore, these semi‐dwarf and increased branching characteristics have the potential to help construct a rapeseed ideotype. Significantly, the editing resources obtained in our study provide desirable germplasm for further breeding of high yield in rapeseed.
Fishing Households, Social Support, and Depression after the Deepwater Horizon Oil Spill
Authors: Parks Vanessa , Slack Tim , Ramchand Rajeev , Drakeford Leah , Finucane Melissa L. , Lee Matthew R. 
Published: August 2019

AbstractThis study contributes to the growing body of knowledge on mental health in the aftermath of disasters by examining depressive symptoms among residents of the U.S. Gulf Coast region 6 years after the onset of the Deepwater Horizon oil spill (DHOS). Using data from the Survey of Trauma, Resilience, and Opportunity in Neighborhoods in the Gulf (STRONG), we test how social support and ties to the fishing industry are related to the likelihood of a positive depression screen. The results show that, among most residents of the Gulf Coast region, social support holds an inverse relationship with the likelihood of a positive depression screen. However, among fishing households, greater social support is associated with a higher probability of screening positive for depression. By showing that fishing households with greater social support are more susceptible to depressive symptoms in this setting, our results uncover a potentially important mechanism that contributes to the unique vulnerability of fishers, which in turn holds implications for differential impacts across social groups in environmental disaster contexts.
Estimation of cyclic long‐memory parameters
Authors: Alomari Huda Mohammed , Ayache Antoine , Fradon Myriam , Olenko Andriy 
Published: August 2019

AbstractThis paper studies cyclic long‐memory processes with Gegenbauer‐type spectral densities. For a semiparametric statistical model, new simultaneous estimates for singularity location and long‐memory parameters are proposed. This generalized filtered method‐of‐moments approach is based on general filter transforms that include wavelet transformations as a particular case. It is proved that the estimates are almost surely convergent to the true values of parameters. Solutions of the estimation equations are studied, and adjusted statistics are proposed. Monte‐Carlo study results are presented to confirm the theoretical findings.
Comparing cannula‐based subcision with the common needle method: A clinical trial
Authors: Nilforoushzadeh Mohammad Ali , Lotfi Elaheh , Heidari‐kharaji Maryam , Nickhah Nahid , Alavi Shiva , Mahmoudbeyk Mona 
Published: August 2019

AbstractSummary of trial designTreatment of depressed acne scars requires multiple modalities. Although needle subcision is a valuable method, it exhibits mild to moderate efficacy in treatment of deep acne scars owing to the high recurrence rate and other side effects. A total of 100 patients with rolling acne scars were randomly treated using the standard technique of 18 gauge cannula and 27 gauge needle subcision in two parallel groups, and the effect of cannula subcision instead of needle subcision was evaluated in the treatment of rolling acne scars.MethodsA total of 100 patients were randomly treated using the standard technique of 18 gauge cannula and 27 gauge needle subcision. The outcomes of these procedures were assessed by three blinded dermatologists and by patients’ satisfaction.ResultsSubcision using the cannula showed good and very good improvement in about 83% of patients (n = 50, P < .05) based on dermatologists investigation and almost no side effects were observed in compared with needle subcision. The response rate was significantly different while using cannula subcision.ConclusionCannula subcision appears to be a safe and practical technique that can enhance the efficacy of subcision without considerable complications.
Importance of authorship and inappropriate authorship assignment in paediatric research in low‐ and middle‐income countries
Authors: Rees Chris A. , Keating Elizabeth M. , Dearden Kirk A. , Haq Heather , Robison Jeff A. , Kazembe Peter N. , Bourgeois Florence T. , Niescierenko Michelle 
Published: August 2019

AbstractObjectiveTo understand the importance of authorship and authorship position, and gauge perceptions of inappropriate authorship assignment, among authors publishing paediatric research conducted in low‐ and middle‐income countries (LMICs).MethodsWe conducted a cross‐sectional, mixed‐methods study using an online survey of both corresponding and randomly selected, non‐corresponding authors who published research conducted in LMICs from 2006 to 2015 in the top four paediatric journals by Eigenfactor score. We used chi‐square tests to compare responses by authors living in LMICs to authors living in high‐income countries (HICs). We analysed qualitative responses using thematic analysis.ResultsOf 1420 potential respondents, 19.6% (n = 279) completed the survey. 57% (n = 159) lived in LMICs and 43% (n = 120) in HICs. LMIC authors more commonly perceived first authorship as most important for their academic advancement than HIC authors (74.2% vs. 60.8%, P = 0.017), while HIC authors reported last authorship as most important (25.1% vs. 38.3%, P = 0.018). 65% (n = 181) of respondents believed that their collaborators had been inappropriately assigned authorship positions (no difference in LMIC and HIC responses) and 32.6% (n = 91) reported personally accepting inappropriate authorship positions (more common in HIC respondents, P = 0.005). In qualitative data, respondents questioned the applicability of standard authorship guidelines for collaborative research conducted in LMICs.ConclusionsLMIC and HIC authors held different perceptions about the importance of authorship position. Reported inappropriate authorship assignment was common among both LMIC and HIC respondents. Alternatives to standard authorship criteria for research conducted in LMICs merit further studies.