bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal single-cell RNA sequencing dataset of gastroesophagus development from embryonic to post-natal stages
Authors: Prakash Pon Ganish , Kumar Naveen , Gurumurthy Rajendra Kumar , Chumduri Cindrilla 
Published: November 2024

AbstractGastroesophageal disorders and cancers impose a significant global burden. Particularly, the prevalence of esophageal adenocarcinoma (EAC) has increased dramatically in recent years. Barrett’s esophagus, a precursor of EAC, features a unique tissue adaptation at the gastroesophageal squamo-columnar junction (GE-SCJ), where the esophagus meets the stomach. Investigating the evolution of GE-SCJ and understanding dysregulation in its homeostasis are crucial for elucidating cancer pathogenesis. Here, we present the technical quality of the comprehensive single-cell RNA sequencing (scRNA-seq) dataset from mice that captures the transcriptional dynamics during the development of the esophagus, stomach and the GE-SCJ at embryonic, neonatal and adult stages. Through integration with external scRNA-seq datasets and validations using organoid and animal models, we demonstrate the dataset’s consistency in identified cell types and transcriptional profiles. This dataset will be a valuable resource for studying developmental patterns and associated signaling networks in the tissue microenvironment. By offering insights into cellular programs during homeostasis, it facilitates the identification of changes leading to conditions like metaplasia and cancer, crucial for developing effective intervention strategies.
Piezo activity levels need to be tightly regulated to maintain normal morphology and function in pericardial nephrocytes
Authors: Schulz Kristina , Hazelton-Cavill Paris , Alornyo Karl K. , Edenhofer Ilka , Lindenmeyer Maja , Lohr Christian , Huber Tobias B. , Denholm Barry , Koehler Sybille 
Published: November 2024

AbstractDue to their position on glomerular capillaries, podocytes are continuously counteracting biomechanical filtration forces. Most therapeutic interventions known to generally slow or prevent the progression of chronic kidney disease appear to lower these biomechanical forces on podocytes, highlighting the critical need to better understand podocyte mechano-signalling pathways. Here we investigated whether the mechanotransducer Piezo is involved in a mechanosensation pathway in Drosophila nephrocytes, the podocyte homologue in the fly. Loss of function analysis in Piezo depleted nephrocytes reveal a severe morphological and functional phenotype. Further, pharmacological activation of endogenous Piezo with Yoda1 causes a significant increase of intracellular Ca++ upon exposure to a mechanical stimulus in nephrocytes, as well as filtration disturbances. Elevated Piezo expression levels also result in a severe nephrocyte phenotype. Interestingly, expression of Piezo which lacks mechanosensitive channel activity, does not result in a severe nephrocyte phenotype, suggesting the observed changes in Piezo wildtype overexpressing cells are caused by the mechanosensitive channel activity. Moreover, blocking Piezo activity using the tarantula toxin GsMTx4 reverses the phenotypes observed in nephrocytes overexpressing Piezo. Taken together, here we provide evidence that Piezo activity levels need to be tightly regulated to maintain normal pericardial nephrocyte morphology and function.
Fabrication of tannic acid-incorporated polyvinylpyrrolidone/polyvinyl alcohol composite hydrogel and its application as an adsorbent for copper ion removal
Authors: Chunhakowit Parichart , Phabjanda Yada , Aunwisat Atchara , Busayaporn Wutthikrai , Songsrirote Kriangsak , Prayongpan Pornpimol 
Published: November 2024

AbstractAn effective tannic acid-incorporated polyvinylpyrrolidone/polyvinyl alcohol composite hydrogel with high-potential sorption capacity was developed for the removal of copper from aqueous solution. The composite hydrogel exhibited pH-dependent swelling, in which swelling and shrinking occurred reversibly with adjustment of the pH of the medium. At pH 4, the maximal adsorption capacity for copper at 30 °C was 297.0 mg g-1, and the adsorbent dose was 4 g L-1. The adsorption kinetics were best fitted with a pseudo-second order kinetic model. The adsorption behavior was well predicted by the Freundlish isotherm. The thermodynamics parameters indicated a spontaneous and exothermic reaction with an increase in the entropy of the system. The chemical changes in the film structure before and after adsorption treatment were characterized by Scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and X-ray absorption spectroscopy (XAS). The FTIR, XPS and XAS results confirmed that Cu bound to the oxygens in the -OH, C = O and N-(C = O)- functional groups on the T-HD. XAS analysis revealed the chemical composition and molecular geometry of the adsorbed copper ions. The single-solute adsorption and coadsorption mechanisms, which provide insight into cobalt-copper, nickel-copper, or nickel-cobalt-copper complex solutions, were investigated. The composite hydrogel exhibited excellent regeneration ability in EDTA solution. Notably, the adsorbent retained an adsorption efficiency exceeding 87% even after five regeneration cycles. On the basis of both adsorbent characteristics and adsorption performance, it was determined that the composite hydrogel has the potential to be used as a platform for developing materials to treat wastewater containing high levels of metal contaminants such as those from the electroplating industry.
Mitochondrial ATP synthesis is essential for efficient gametogenesis in Plasmodium falciparum
Authors: Sparkes Penny C. , Famodimu Mufuliat Toyin , Alves Eduardo , Springer Eric , Przyborski Jude , Delves Michael J. 
Published: November 2024

AbstractPlasmodium male and female gametocytes are the gatekeepers of human-to-mosquito transmission, therefore essential for propagation of malaria within a population. Whilst dormant in humans, their divergent roles during transmission become apparent soon after mosquito feeding with a rapid transformation into gametes – males forming eight motile sperm-like cells aiming to fertilise a single female gamete. Little is known about how the parasite fuels this abrupt change, and the potential role played by their large and elaborate cristate mitochondrion. Using a sex-specific antibody and functional mitochondrial labelling, we show that the male gametocyte mitochondrion is less active than that of female gametocytes and more sensitive to antimalarials targeting mitochondrial energy metabolism. Rather than a vestigial organelle discarded during male gametogenesis, we demonstrate that mitochondrial ATP synthesis is essential for its completion. Additionally, using a genetically encoded ratiometric ATP sensor, we show that gametocytes can maintain cytoplasmic ATP homeostasis in the absence of mitochondrial respiration, indicating the essentiality of the gametocyte mitochondrion for transmission alone. Together, this reveals how gametocytes responsively balance the conflicting demands of a dormant and active lifestyle, highlighting the mitochondria as a rich source of transmission-blocking targets for future drug development.
Habitat suitability models reveal extensive distribution of deep warm-water coral frameworks in the Red Sea
Authors: Nolan Megan K. B. , Marchese Fabio , Purkis Sam J. , Ouhssain Mustapha , Kheireddine Malika , Terraneo Tullia I. , Chimienti Giovanni , Rodrigue Mattie , Eweida Ameer A. , Jones Burton , Benzoni Francesca 
Published: November 2024

AbstractDeep-sea coral frameworks are understudied in the Red Sea, where conditions in the deep are conspicuously warm and saline compared to other basins. Habitat suitability models can be used to predict the distribution pattern of species or assemblages where direct observation is difficult. Here we show how coral frameworks, built by species within the families Caryophylliidae and Dendrophylliidae, are distributed between water depths of 150 m and 700 m in the northern Red Sea and Gulf of Aqaba. To extrapolate the known (ground-truthed) positions of these deep frameworks, we use environmental and geomorphometric variables to inform well-performing maximum entropy models. Over 250 km2 of seafloor in our study area are identified as suitable for such frameworks, equivalent to at least 35% of the area of photic-zone coral reefs in the same region. We hence contend that deep-water coral frameworks are an important and underappreciated repository of Red Sea biodiversity.
A DCNN‐LSTM model for the construction of the road radio map
Authors: Luo Yechao , Shao Wei , Jin Jun , Liu Yang , Lu Haizhou , Zou Hang , Feng Mengting , Zhang Peng 
Published: November 2024

AbstractIt is crucial for vehicular communications to optimize the field strength coverage on roads, which can be illustrated by the radio map (RM). In this paper, a deep convolutional neural network‐long short‐term memory (DCNN‐LSTM) model for the construction of the road RM is proposed. First, a multi‐modal dataset is built, including the measured field strength, longitude, latitude and elevation data obtained at various measurement points, as well as the outline maps of the buildings that are close to the measurement points. Second, the DCNN‐LSTM model is designed to extract electromagnetic and geographical features separately from the multi‐modal input data and fuse them to predict the field strength at unmeasured points. Finally, the road RM is constructed using the measured and predicted field strength data. The simulation results demonstrate that the field strength prediction accuracy of the proposed model is better than that of the ordinary kriging and the traditional deep learning models. Moreover, it exhibits robust performance even in noise interference environments. The constructed RM also provides an intuitive visualization of field strength coverage along the target road.
Variance‐driven security optimisation in industrial IoT sensors
Authors: Gupta Hardik , Singh Sunil K. , Kumar Sudhakar , Sharma Karan , Saini Hardeep , Gupta Brij B. , Arya Varsha , Chui Kwok Tai 
Published: November 2024

AbstractThe Industrial Internet of Things (IIoT) has transformed industrial operations with real‐time monitoring and control, enhancing efficiency and productivity. However, this connectivity brings significant security challenges. This study addresses these challenges by identifying abnormal sensor data patterns using machine learning‐based anomaly detection models. The proposed framework employs advanced algorithms to strengthen industrial defences against cyber threats and disruptions. Focusing on temperature anomalies, a critical yet often overlooked aspect of industrial security, this research fills a gap in the literature by evaluating machine learning models for this purpose. A novel variance‐based model for temperature anomaly detection is introduced, demonstrating high efficacy with accuracy scores of 0.92 and 0.82 on the NAB and AnoML‐IOT datasets, respectively. Additionally, the model achieved F1 scores of 0.96 and 0.89 on these datasets, underscoring its effectiveness in enhancing IIoT security and optimising cybersecurity for industrial processes. This research not only identifies security vulnerabilities but also presents concrete solutions to improve the security posture of IIoT systems.
Intrusion detection in cluster‐based wireless sensor networks: Current issues, opportunities and future research directions
Authors: John Ayuba , Isnin Ismail Fauzi Bin , Hamid Hussain Madni Syed , Faheem Muhammed 
Published: November 2024

AbstractWireless sensor network (WSN) cluster‐based architecture is a system designed to control and monitor specific events or phenomena remotely, and one of the important concerns that need quick attention is security risks such as an intrusion in WSN traffic. At the same time, a high‐level security method may refer to an intrusion detection system|intrusion detection systems (IDS), which may be employed effectively to achieve a higher level of security in detecting an intruder attack or any attack initiated within a WSN system. The significance of the detection of network intrusions on heterogeneous cluster‐based sensor networks with wireless connections, as well as the approaches to machine learning utilised in IDS model development, were discussed. In addition, this research conducted several comparative studies of feature selection techniques and machine learning methodologies in the development of intrusion detection systems. The authors used a bibliometric indicator to identify the leading trends when it comes to IDS, and the VOS viewer was used to create a spatial mapping of co‐authorship, co‐occurrence, and citation types of analysis with their respective units of study. The purpose of this research paper is to generate relevant findings and a research problem formulation that can lead to a research gap in the research topic's domain area.
Hitzeschäden vermeiden
Authors: Bierbach Elvira 
Published: November 2024

SummaryHohe Temperaturen können schnell zur Gefahr werden. Beispielsweise verliert der Körper durch gesteigertes Schwitzen Wasser und wichtige Elektrolyte. Es ist wichtig, Hitzefolgen zu vermeiden, sie frühzeitig zu erkennen und rechtzeitig Erste Hilfe zu leisten. Doch was genau sind die Anzeichen, und wie kann man richtig handeln?
Klimakterium virile – die Andropause
Authors: Stern Apothekerin Cornelia , Blume Ralf , Jahn Claus , Löwensprung Natalie Rosenhauer-Von 
Published: November 2024

Summary4 Expert*innen stellen Ihnen Behandlungsstrategien aus der Gemmopathie, Klassischen Homöopathie, Komplexhomöopathie und Anthroposophie vor.
Feiner Rauch für dicke Luft
Authors: Koppold Anna 
Published: November 2024

SummarySeit Jahrhunderten wird das Räuchern mit Kräutern auf der ganzen Welt für die atmosphärische Reinigung von Häusern und Räumen genutzt, um angestaute, verdichtete Energien aufzulösen. So entsteht eine angenehme, entspannte Atmosphäre.
Gemäß der Wetterlage …
Authors: Welsch Katja 
Published: November 2024

SummaryModalitäten sind in der Homöopathie die oft besonderen Begleitumstände, unter denen sich Symptome bessern oderverschlimmern können. Bedingungen wie Temperatur, Wetter und Mondphasen werden in der Anamnese aufgespürt, mit der Arzneimittellehre abgeglichen und bei der Mittelwahl beachtet. Im Fallbeispiel wird einer Patientin mit Causticum geholfen, das zu den in der Anamnese geschilderten Gelenkbeschwerden sowie den Wetterverhältnissen passend erscheint.
Klimaneutrale Praxis: Märchen oder Zukunftsmodell?
Authors: Köhler Daniel 
Published: November 2024

SummaryDie Ambition der Klimaneutralität geriet aufgrund inflationärer Verwendung in der Vergangenheit in die Kritik, sollte aber dennoch mehr denn je intern umgesetzt und extern kommuniziert werden. Die klimaneutrale Heilpraktikerpraxis führt zu Vertrauensbildung, Transparenz, kurz- und langfristigen Kosteneinsparungen, gesünderen Bedingungen für Mitarbeiter und besserer öffentlicher Wahrnehmung. Die Umstellung einer Praxis auf Klimaneutralität erfordert eine Treibhausgasbilanz (CO2-Fußabdruck), eine Wesentlichkeitsanalyse zur Feststellung der effizientesten und wirtschaftlich möglichen Maßnahmen sowie einen Klimaneutralitätsplan unter Einbeziehung von Förder- und Einsparmöglichkeiten.
Zu heiß, zu kalt: Das Klima und der Körper
Authors: Schmid Eva C. 
Published: November 2024

SummaryDer Klimawandel betrifft nicht allein die Umwelt. Vor allem Extremtemperaturen wirken sich auf jeden menschlichen Organismus aus. Hitze und Kälte können die Gesundheit unmittelbar beeinflussen oder bestehende Beschwerden verschlimmern. Sie verursachen immer häufiger medizinische Notfälle. Der Beitrag betrachtet außerdem die Bedeutung von Temperaturen für die Einnahme und Wirkweisen von Medikamenten sowie für das Infektionsgeschehen.
Aktuelle Empfehlungen zur Lungenfunktionsdiagnostik – Kurzfassung
Authors: Criée Carl-Peter , Smith Hans-Jürgen , Preisser Alexandra M. , Bösch Dennis , Butt Uta , Borst Matthias M. , Hämäläinen Nina , Husemann Kim , Jörres Rudolf A. , Kardos Peter , Lex Christiane , Meyer F. Joachim , Nachtigall Detlev , Nowak Dennis , Ochmann Uta , Randerath Winfried , Schütz Ansgar , Schucher Bernd , Spiesshoefer Jens , Taube Christian , Walterspacher Stephan , Wollsching-Strobel Maximilian , Worth Heinrich , Gappa Monika , Windisch Wolfram ,   Deutsche Gesellschaft für Pneumologie und Beatmungsmedizin e. V. (DGP),   Deutsche Atemwegsliga (DAL),   Deutsche Lungenstiftung (DLS) sowie,   Deutsche Gesellschaft für Arbeitsmedizin und Umweltmedizin (DGAUM)
Published: November 2024

ZusammenfassungDie aktualisierten Empfehlungen zur Lungenfunktion wurden zum Jahreskongress der Deutschen Gesellschaft für Pneumologie und Beatmungsmedizin (DGP) 2024 vorgestellt und sind in der Zeitschrift „Atemwegs- und Lungenkrankheiten, Jahrgang 50 (2024) März (111–184)“, publiziert, wo sie als PDF kostenlos abrufbar sind. Diese Empfehlungen integrieren auch neue Ansätze zur Interpretation von Funktionsuntersuchungen, wie sie von der ERS (European Respiratory Society) in Zusammenarbeit mit der ATS (American Thoracic Society) publiziert worden sind. Dies betrifft die Spirometrie, die Bodyplethysmografie, die Diffusionskapazitätsmessung, den Bronchodilatationstest, die unspezifische bronchiale Provokation sowie die Referenzwerte. Die Empfehlungen adressieren auch die Oszillometrie sowie die atemmuskuläre Funktionsdiagnostik. Die aktuell vorliegende Publikation versteht sich als Kurzversion der wesentlichen Inhalte dieser komplexen Empfehlungen zur Lungenfunktionsdiagnostik, weist aber auch darauf hin, dass dies nicht das Studium der Langversion der aktuellen Empfehlungen ersetzt, was es insbesondere vor dem Hintergrund der großen Bedeutsamkeit der Lungenfunktionsdiagnostik im klinisch-pneumologischen Alltag zu betonen gilt.
First Steps Toward a Participatory Research Program: Early Intervention Perspectives on SLP Services for Children of Diverse Backgrounds
Authors: Kover Sara , Arora Natasha , Barton Hannah , Roberts Carly 
Published: November 2024

AbstractThis research was designed to deepen the understanding of contributing factors to disparities in speech-language pathology (SLP) service delivery to children of diverse backgrounds with language and communication delays, as well as to initiate community connections. We sought to understand the perspectives and experiences of those who provide or oversee SLP services to children with communication disorders in early intervention settings in Washington State. Individual semistructured interviews were completed with six SLPs and three administrators across five organizations. The interviews were designed to understand both individual- and systems-level contributing factors to service delivery challenges. Interviews were analyzed using an iterative coding process with multiple rounds of coding and multiple coders. In addition to synthesizing participants' understandings of, and preparation for, culturally responsive practice, three overarching themes are presented: (1) assets of early intervention as a service delivery model, (2) the distance between families and the practitioners and systems that serve them, and (3) compromised access and quality of services due to a predominantly monolingual-English system. Findings inform the next steps for building community partnerships with early intervention organizations. The long-term goal of this work is to collaboratively develop a participatory research program on equitable service delivery that addresses children's, families', and SLPs' needs.
Total Elbow Replacement in Swine: Surgical Technique and Initial Clinical Experience in a Kunekune Sow
Authors: H. Santiago Mejia , McOnie Rebecca C. , Bellefeuille Sean D. , Tinga Selena , Guiot Laurent P. 
Published: November 2024

Abstract
Objective The aim of this study was to describe the development of a total elbow replacement (TER) technique in swine and report the outcome of TER in a Kunekune sow (61 kg) with severe elbow osteoarthritis.
Methods The technique was developed by operating on three pig cadavers (Yorkshire 40–50 kg). The TATE Elbow implant fit was confirmed with modeling to the patient's computed tomography imaging. Patient-specific instrumentation was printed for attempted use. Total elbow replacement was performed using a lateral approach, including lateral epicondylar osteotomy, careful retraction of the radial nerve, and preservation of the lateral collateral ligament. Postoperative care included controlled exercise and physical therapy.
Results A lateral approach to the pig elbow for TATE TER was successfully developed. The bulk of the lateral head of the triceps muscle precluded use of the patient-specific instrumentation; the procedure was performed using standard instrumentation. Radiographic evidence up to 9 months postoperatively showed adequate implant alignment and satisfactory osteointegration of humeral and radioulnar components. There were not any postoperative complications and the patient's comfort was improved by 6 months and sustained at 18 months.
Conclusion A lateral approach for TATE TER in a pig with advanced elbow osteoarthritis proved feasible and effective. With the expansion of the TATE Elbow line, it is possible that a larger range of pigs could be treated. This report suggests that a lateral approach for TATE TER in dogs could represent a suitable alternative to the medial approach currently used.
Biomechanical Comparison of Spacer Pin Fixation to Two Established Methods of Tibial Tuberosity Transposition Stabilization in Dogs
Authors: Sullivan Anna L. , Ordway Nathaniel R. , Park Brian H. , Natsios Pavlos , Petazzoni Massimo , Tinga Selena 
Published: November 2024

Abstract
Objective The aim of this cadaveric study was to compare the biomechanical outcomes of three methods of stabilization for tibial tuberosity transposition to treat medial patellar luxation: a complete osteotomy with a two-pin and tension band wire (TBW) fixation (TBW group), a partial osteotomy with a two-pin fixation (2 Pin group), and a partial osteotomy with a spacer pin fixation (Spacer Pin group).
Study Design Thirty medium to large-sized canine cadaveric tibiae were dissected and randomly assigned to one of three groups: TBW, 2 Pin, and Spacer Pin groups. The patellar ligaments were loaded in tension until ultimate failure. Ultimate failure force and mode of failure were documented, stiffness was calculated, and the results were compared statistically between the three treatment groups.
Results There were not any significant differences in ultimate failure force or stiffness between groups. All groups predominantly failed by patellar ligament failure, with distal tibial crest fracture/displacement being the second-most common mode in the 2 Pin and Spacer Pin groups.
Conclusion The mechanical properties of the spacer pin stabilization were not different from the TBW and 2 Pin groups. The spacer pin technique could be an alternative way to stabilize tibial tuberosity following tibial tuberosity transposition with a partial osteotomy based on this cadaveric load-to-failure model.