bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns of genetic variation and parentage in a population of desert kit foxes (Vulpes macrotis arsipus) in California
Authors: Honeycutt Rodney L , Monty Olivier , Vanherweg William , Randel Charles J 
Published: December 2024

Abstract
Kit foxes represent a small canid in semi-arid and arid regions in the southwestern United States. The San Joaquin Kit Fox (Vulpes macrotis mutica) is listed as federally endangered, and based on more recent studies, populations of the Nevada Kit Fox (V. m. nevadensis) are declining in Utah. These 2 subspecies show a loss of genetic variation in response to population declines. Various anthropogenic activities threaten kit foxes throughout other regions of their distribution, but less is known about the basic biology and genetics of other subspecies of kit foxes. Here, we used sequences from the mitochondrial cytochrome b (Cytb) gene and 12 DNA microsatellites to investigate patterns of genetic variation and test for mating fidelity in a population of the socially monogamous Desert Kit Fox, V. m. arsipus. In the current study, the mitochondrial haplotype diversity (Hd) was higher than or comparable to other canids and terrestrial mammals. The mitochondrial results also provided evidence for male-biased dispersal and female philopatry. Expected heterozygosity (He) and number of alleles per locus (Na) for the 12 microsatellite loci were higher relative to other species of foxes and canids. As seen for nearly all species of socially monogamous canids, 17% of the pups of the mated pairs of the Desert Kit Fox revealed evidence of extra-pair mating, a value like small canids. Additionally, we observed 1 case of mate switching between breeding seasons, even though the previous mate was still alive. These data suggest that long-term pair bonding may not be as extensive as once thought.
Neurological Disorders and Use of Healthcare Services After Bacterial Meningitis in Childhood: A Nationwide, Population-Based Cohort Study
Authors: Graham Emma E , Tetens Malte M , Bodilsen Jacob , Andersen Nanna S , Dessau Ram , Ellermann-Eriksen Svend , Franck Kristina , Midgley Sofie , Møller Jens Kjølseth , Nielsen Alex Christian , Nielsen Lene , Søgaard Kirstine K , Østergaard Christian , Lebech Anne-Mette , Nygaard Ulrikka , Omland Lars H , Obel Niels 
Published: December 2024

Abstract
In this population-based, nationwide Danish cohort study, bacterial meningitis in childhood was associated with increased neurological morbidity. The risks were highest among young children, who also received more antiepileptic medication. All children had increased frequency of hospital visits. These findings highlight the importance of follow-up after bacterial meningitis in children.
Clinical workload profile of medical physics professionals at particle therapy Centers: a National Survey in Japan
Authors: Ota Seiichi , Yasui Keisuke , Ogata Toshiyuki , Mori Yutaro , Nishio Teiji , Tohyama Naoki , Okamoto Hiroyuki , Kurooka Masahiko , Shimomura Kohei , Kojima Toru , Onishi Hiroshi 
Published: December 2024

Abstract
The current research on staffing models is primarily focused on conventional external photon beam therapy, which predominantly involves using linear accelerators. This emphasizes the need for comprehensive studies to understand better and define specific particle therapy facilities’ staffing requirements. In a 2022 survey of 25 particle therapy facilities in Japan with an 84% response rate, significant insights were obtained regarding workload distribution, defined as the product of personnel count and task time (person-minutes), for patient-related tasks and equipment quality assurance and quality control (QA/QC). The survey revealed that machinery QA/QC tasks were particularly demanding, with an average monthly workload of 376.9 min and weekly tasks averaging 162.1 min. In comparison, patient-related workloads focused on treatment planning, exhibiting substantial time commitments, particularly for scanning and passive scattering techniques. The average workloads for treatment planning per patient were 291.3 and 195.4 min, respectively. In addition, specific patient scenarios such as pre-treatment sedation in pediatric cases require longer durations (averaging 84.5 min), which likely include the workloads of not only the physician responsible for sedation but also the radiotherapy technology and medical physics specialists providing support during sedation and the nursing staff involved in sedation care. These findings underscore the significant time investments required for machinery QA/QC and patient-specific treatment planning in particle therapy facilities, along with the need for specialized care procedures in pediatric cases. The results of this survey also emphasized the challenges and staffing requirements to ensure QA/QC in high-precision medical environments.
Dieulafoy lesion with rare vascular complications: diagnostic and therapeutic approach
Authors: Abdoh Qusay , Samara Enas , Zayed Alaa , Khader Mohammed , Maqboul Iyad , Rashed Mohammed A M 
Published: December 2024

Abstract
Dieulafoy lesions are a rare cause of gastrointestinal bleeding, characterized by an enlarged submucosal blood vessel that bleeds without visible abnormalities. The diagnosis is typically made via endoscopy, and treatment usually involves endoscopic therapy. This case involves a 46-year-old female who presented with upper gastrointestinal bleeding due to a Dieulafoy’s lesion, treated with band ligation and later embolization after the lesion was found to originate from the left phrenic artery. The patient developed rare complications, including splenic infarction and pleural effusion, which required additional management. Upon initial investigations, the patients had an accidental finding on computed tomography of asymptomatic celiac artery compression that was advised to take into consideration for the possibility of developing median arcuate ligament syndrome and was treated conservatively. The case highlights the diagnostic and therapeutic challenges of Dieulafoy’s lesion and underscores the need for individualized treatment and further research.
(005) ENDOTHELIUM-DERIVED 6-NITRODOPAMINE RELAXES THE HUMAN CORPUS CAVERNOSUM
Authors: Lorenzon F , Caliani Mathias-Netto F , Placco Araujo Glina F , Glina S , Paiva O , Fregonesi A , De Miranda Cará A , Cintra Júnior W , Itocazo Rocha R , De Nucci G 
Published: December 2024

Abstract

Introduction
Endothelium-derived 6-nitrodopamine (6-ND) has been identified as the most potent endogenous relaxant agent in the rabbit corpus cavernosum, presenting a novel mechanism by which endothelium-derived and nitrergic NO induces corpus cavernosum relaxation.

Objective
To investigate the release and action of 6-ND in human corpus cavernosum.

Methods
Human corpus cavernosum (HCC) was obtained from 13 patients (mean age: 45 years) who had undergone male-to-female gender reassignment surgery. All the patients received antiandrogen and estrogen treatment for at least two years before surgery. One HCC was suspended in a 3-ml glass chamber containing Krebs–Henseleit solution (KHS), warmed to 37°C and oxygenated with a gas mixture of 95% O₂ and 5% CO₂, supplemented with ascorbic acid (3 mM) to prevent catecholamine oxidation. The tissues were incubated for 30 minutes with NO synthase inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME-100 μM) and the voltage-gated sodium channel blocker tetodrotoxin (TTX-1 μM). Subsequently, samples of the KHS were transferred to amber Eppendorf vials and stored at −80°C for subsequent analysis by liquid chromatography–tandem mass spectrometry (LC–MS/MS). For functional evaluation, the HCC strip (1.5 cm length) was mounted vertically in a glass chamber tissue bath containing 10 mL of KHS at 37°C, with a fine sewing thread, one end attached through a loop to a metal hook and the other end tied to an isometric force transducer. The tissues were allowed to equilibrate for 45 minutes under a resting tension of 10 mN, and the isometric tension was registered using a PowerLab system (ADInstruments). The strips were precontracted with the thromboxane A2 mimetic U-46619 (30 nM) and cumulative concentration-response curves to 6-ND (1 nM-100 μM) was performed to investigate the relaxation induced by this drug.

Results
6-ND was detected in HCC via LC–MS/MS. Incubation with L-NAME significantly decreased 6-ND levels (0.6 ± 0.2 vs 0.4 ± 0.4, ng/mL; n = 8), while incubation with TTX had no effect on 6-ND release (n = 6). In HCC pre-contracted strips, incubation with 6-ND produced concentration-dependent relaxations with pEC50 and Emax values of 7.3 ± 0.4 and 35.1 ± 14.5%, respectively (n = 5).

Conclusions
The findings establish 6-ND as a novel endogenous non-neurogenic mediator of HCC relaxation, establishing a new mechanism of action for NO in HCC relaxation.

Disclosure
No.
(018) A PILOT STUDY OF ONLINE GROUP COGNITIVE-BEHAVIORAL PSYCHOTHERAPY AND SEX EDUCATION FOR BRAZILIAN WOMEN WITH SEXUAL DYSFUNCTION OR ISSUES
Authors: Bonato F , Mussi A , Pellizzer C , Gund B , Genez L , Gobbi R , Cardoso N 
Published: December 2024

Abstract

Introduction
Group psychotherapy (GP) is a modality of health intervention whose benefits and effectiveness have been demonstrated in a variety of studies, but there there is still a scientific gap when it comes to online GP interventions for the treatment of female sexual dysfunctions (FSD).

Objective
This study aimed to assess the efficacy of an online group cognitive-behavioral therapy (GCBT) combined with sex education intervention for Brazilian women experiencing sexual issues or female sexual dysfunction (FSD).

Methods
Using a quasi-experimental design, a single-group pre-posttest intervention was conducted over 12 weeks. Seven women aged 25-58, reporting female sexual issues and/or FSD, participated in weekly online psychotherapy sessions. Sexual function and satisfaction were evaluated using the Female Sexual Function Index (FSFI) and Female Sexual Quotient (FSQ).

Results
Following the intervention, there was a significant improvement in overall sexual function among participants (d = 1.29). Substantial effect sizes were observed particularly in desire (d = 1.53), satisfaction measured by both FSQ (d = 1.55) and FSFI (d = 1.41), as well as arousal (d = 1.21) and lubrication (d = 1.16). Moderate effects were noted for orgasm (d = .66). However, no significant differences were found in sexual function scores when controlling for variables such as medication use, diagnoses, and occupation status. The intervention did not show efficacy in reducing pain associated with sexual activity.

Conclusions
This pilot study highlights the feasibility and effectiveness of a brief online GCBT and sex education program in improving sexual function and satisfaction among women facing sexual issues or FSD. Keywords: Cognitive Behavior Therapy; Sex Education; Sexual Health; Sexual Dysfunction; Group Psychotherapy; Online Therapy.

Disclosure
No.
(020) BRIDGING GAPS: EXPLORING SEXUAL HEALTH EDUCATION PROVISION AMONG TUNISIAN PRIMARY HEALTHCARE PROVIDERS
Authors: Makhlouf E , Kaabia O , Smaali MH , Ben Hassine SA , Cherif W 
Published: December 2024

Abstract

Introduction
Many studies have shown that approaching sexuality during consultations is influenced by different barriers including cultural specificities and the anxiety health professionals experience towards this sensitive topic.

Objective
Since primary healthcare encompasses a crucial preventative role, our aim was to explore the information shared with patients by physicians regarding sexual health. We examined the correlation between sexual health education provision during medical visits with the training of doctors, their perception of their training, and their attitudes toward addressing sexual health matters.

Methods
We conducted this cross-sectional study among Tunisian family medicine physicians and residents, using an anonymous online self-administered questionnaire in French.

Results
The study involved 400 participants, predominantly female (72.1%). The sample included general practitioners (40.8%), family medicine residents (38.2%), and physicians (21%). Regarding sexual health training, 55.4% of the respondents deemed their training “unsatisfactory” or “absent”. Over 85% believed the basic medical curriculum lacked knowledge for sexual history taking and ¾ felt it didn’t cover sexual dysfunctions adequately. For STIs screening and treatment, 34.7% felt their training was insufficient. Regarding patient education, 16.9% of the participants never discussed risky sexual behaviors, while a majority frequently provided information on contraceptive methods (44.4% “often,” 14.1% “always”) as well as STIs protective measures (40%). As shown by the graph below, over 36% never provided information concerning sexual well-being to their patients. Regarding general sexual and reproductive health services, 40.8% never provided information. When it comes to sexual violence, the majority reported providing information infrequently or never (34.1%). Statistically significant relationships were found between receiving sexual health training as well as having a positive perception of sexual health training and skills and a higher provision of educational information (p &lt; 0.05).

Conclusions
Our study highlights gaps in sexual health training and information provision among Tunisian family physicians and residents. Discussions about sexual well-being, violence and reproductive health services remain rare. Our results advocate for improvements in sexual health training and capacitation of primary healthcare professionals in order to enhance the quality of sexual healthcare in Tunisia.

Disclosure
No.
(026) PENILE REHABILITATION AFTER RADICAL PROSTATECTOMY USING LOW-INTENSITY SHOCKWAVE THERAPY FOR ERECTILE FUNCTION: LATE RESULTS FINDINGS FROM A RANDOMIZED CLINICAL TRIAL
Authors: Lessa P , Seligra Lopes L , Salles M , Cordeiro R , Pazeto C , Baccaglini W , Glina S 
Published: December 2024

Abstract

Introduction
There is still few evidence to support the use of low-intensity extracorporeal shockwave therapy (LiESWT) in the management of erectile dysfunction (ED) after radical prostatectomy (RP).

Objective
The aim of this study is to assess the long-term results of LiWEST as a penile rehabilitation method in patients who underwent RP.

Methods
This is an assessment of the late results outcomes of our previous clinical trial registered in ReBEC (ensaiosclinicos.gov.br) RBR-85HGCG. Patients were randomized in two groups with 2 parallel arms and an allocation ratio of 1:1. The control group received tadalafil 5 mg/day, while the experimental group were submitted to tadalafil 5 mg/day plus 2400 shocks/session-week distributed on 4 different penile regions across 8 weeks of treatment. Both groups started the penile rehabilitation after the removal of the transurethral catheter. Sexual function was assessed by IIEF-5 score.

Results
Among 77 patients included in our previous analysis, 35 patients were reassessed after a median follow-up of 45 months (42-50 months; CI 95%). 17 of them had to be excluded from the analysis for biochemical recurrence and/or new interventions. Remaining 7 and 11 patients in the experimental and control group, respectively. The final mean IIEF-5 score between experimental and control groups showed a difference in favor of the LiWEST group, not statistics significantly (18.85, SD 5.66, 95% CI 13.61-24.1 x 16.63, SD 5.48, 95%CI 12.9-20.31; respectively; p = 0.42). Subgroup analysis in patients still using IPDE5 there is a higher difference between mean IIEF5, however not statistics significantly (18.5, SD 6.12, 95%CI 12.07-24.92 x 15.85, SD 3.62, 95% CI 12.50-19.20; p = 0.35).

Conclusions
LiESWT showed a slight benefit on sexual function rehabilitation in patients submitted to RP after a long-term evaluation in the sexually active subgroup which uses daily IPDE5. A larger sample is necessary in further trials to evaluate these results.

Disclosure
No.
(032) ENHANCING THE THERAPY JOURNEY: INTEGRATING AI INTO SEXUAL DYSFUNCTION TREATMENT AT QUAREB
Authors: Issa M 
Published: December 2024

Abstract

Introduction
In the evolving landscape of psychosexual therapy, Artificial Intelligence (AI) offers unprecedented opportunities to enhance therapeutic outcomes. Utilizing AI, a pioneering online platform has transformed the therapy journey for clients experiencing sexual dysfunctions, optimizing interactions between clients, therapists, and therapeutic mediums.

Objective
To demonstrate how AI integration into a psychosexual therapy platform enhances the efficacy of treatments, reduces therapy duration, and supports therapists in clinical decision-making, ultimately improving patient and therapist experiences.

Methods
The platform employs AI in several key areas: 1. Client Interface: AI-driven self-assessment tools help clients understand their sexual dysfunctions before and during the therapy process. AI also generates personalized questions to encourage deeper exploration of their issues. 2. Therapist Support: AI aids therapists by suggesting targeted questions, selecting appropriate interventions, and recommending laboratory investigations based on AI analysis of client data. 3. Medium Analysis: The platform analyzes therapy sessions in real-time, providing therapists with feedback and suggesting effective tools and interventions based on the conversation’s context and progress. Data was collected on therapy outcome efficacy, session duration, client satisfaction, and therapist feedback regarding AI utility.

Results
Clients reported a faster understanding of their sexual issues, reflected by a reduced average number of sessions needed to reach significant therapeutic milestones. Therapists noted improved clinical outcomes due to AI-assisted personalization of therapy approaches and more precise diagnostic support. The medium’s ability to analyze and adapt therapy flows in real-time was found to significantly contribute to the overall effectiveness of sessions.

Conclusions
While human intervention remains irreplaceable in therapy, AI significantly enhances both the patient and therapist experience, making psychosexual therapy more efficient and tailored. The integration of AI into sexual dysfunction therapy not only facilitates a deeper understanding of complex sexual health issues but also streamlines the therapeutic process, ensuring that treatments are more accessible, timely, and effective. Future initiatives will focus on expanding AI capabilities to encompass a broader range of psychosexual disorders and integrating more comprehensive diagnostic tools. This abstract aims to clearly highlight the multifaceted benefits of AI in enhancing the therapeutic journey, based on the practical experiences from Quareb.

Disclosure
No.
(033) UTILIZING ARTIFICIAL INTELLIGENCE TO SUPPORT MEN WITH ERECTILE DYSFUNCTION: A NOVEL MOBILE APPLICATION APPROACH
Authors: Batandjiovski S 
Published: December 2024

Abstract

Introduction
Erectile dysfunction (ED) poses significant challenges to men’s physical and emotional wellbeing, with prevalence increasing with age, affecting approximately 50% of men aged 40 to 70 years. Various factors such as lifestyle habits, medical conditions, and psychological factors influence ED severity, highlighting the need for personalised interventions.

Objectives
This abstract proposes the development of a innovative mobile application leveraging artificial intelligence (AI) to provide comprehensive support for individuals suffering from ED, targeting improved treatment adherence, symptom management, and quality of life enhancement. By integrating parameters such as International Index of Erectile Function (IIEF-5) score, BMI index, activity and exercise levels, calorie intake, smoking status, medications, history of previous surgeries, and current medical conditions, the app aims to offer tailored recommendations to individual needs and circumstances.

Methods
The proposed app will use AI algorithms to analyse self reported user data, including the aforementioned parameters, alongside medical history, lifestyle factors, and psychological aspects, to offer personalised recommendations and interventions. Data input mechanisms will be user-friendly and intuitive, facilitating smooth integration of relevant information into the app’s algorithms. Key features of the application may include discreet symptom tracking, medication reminders (with dosages adjusted based on individual responses and side effects), lifestyle modification suggestions tailored to user preferences and goals, and access to expert advice through teleconsultation services, with specialists in urology and sexual health. The app will prioritize user privacy and confidentiality, adhering to stringent data protection protocols compliant with healthcare regulations such as HIPAA and GDPR, ensuring the security of sensitive information and maintaining anonymity during interactions with healthcare providers.

Results
The results of the preliminary testing of the mobile application will be showen here after the investigation and feedback is received.

Conclusions
This innovative approach holds promise in enhancing accessibility to comprehensive support for men with ED, empowering them to take proactive steps towards improving their sexual health and overall quality of life. By using AI and integrating diverse parameters, the app not only addresses the complex nature of ED but also facilitates personalised care and treatment optimisation, ultimately improving patient outcomes and satisfaction.

Disclosure
No.