bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnetic resonance neurography appearance and diagnostic evaluation of peripheral nerve sheath tumors
Authors: Zhai Heng , Lv Yinzhang , Kong Xiangquan , Liu Xi , Liu Dingxi 
Published: May 2019

AbstractImaging appearances of peripheral nerve sheath tumors by MRI are difficult distinguish from soft-tissue tumors. The objective of this study was to evaluate the feasibility and imaging appearance of high-resolution 3-T magnetic resonance neurography (MRN) of the diagnosis of peripheral nerve sheath tumors (PNSTs) using sampling perfection with application-optimized contrasts using different flip angle evolution (SPACE) sequences. We retrospectively evaluated the MRI and 3D Short tau inversion recovery sampling perfection with application-optimized contrasts using varying flip-angle evolutions (3D-STIR SPACE) sequences of 30 patients with PNSTs diagnosed by surgery and pathology. The contrast-enhanced 3D-STIR SPACE images were retrospectively analyzed and evaluated for the visualization of PNSTs. The tumors were evaluated by their number, location, morphology, size, signal intensity and enhancement characteristics. The imaging findings and characteristic signs of conventional MRI scanning and contrast-enhanced 3D-STIR SPACE sequences were compared. In these cases, conventional MRI images display the location, number, shape, size and signal characteristics of the lesions. These tumors were mostly solitary and had a well-defined boundary. Compared to conventional MRI images, imaging appearances including neurogenic origin, length of the peripheral nerves and relation to the nerve of PNSTs on 3D-STIR SPACE images were more accuracy (P < 0.05). Compared to 3D-STIR SPACE images, contrast-enhanced images can more clearly display background suppression of the peripheral nerves. The “split fat” sign and “target” sign were seen in some patients. 3D STIR SPACE sequences demonstrate its significant capacity to diagnostic evaluate and location of PNSTs. This article comprehensively reviews radiologic findings and illustrates the MRN features of PNSTs. 3D-STIR SPACE sequences be used for preoperative evaluation of PNSTs.
Lithospheric delamination and upwelling asthenosphere in the Longmenshan area: insight from teleseismic P-wave tomography
Authors: He Chuansong , Dong Shuwen , Wang Yanghua 
Published: May 2019

AbstractWe apply teleseismic P-wave tomography to reconstruct the velocity structure of the Longmenshan area. Our results show possible large-scale delamination beneath the Songpan-Ganzi and Qiangtang terranes, which induced upwelling asthenosphere. Upwelling asthenosphere might have led to lower crust heating, facilitating eastward extrusion of the Songpan Ganzi terrane resulting in localized deformation and uplift along the Longmenshan orogenic belt. We suggest that the eastward extrusion of the Songpan-Ganzi terrane against the rigid lithospheric root of the Sichuan Basin results in stress accumulation and release, leading to large earthquakes in the Longmenshan area.
In vivo regeneration of interspecies chimeric kidneys using a nephron progenitor cell replacement system
Authors: Fujimoto Toshinari , Yamanaka Shuichiro , Tajiri Susumu , Takamura Tsuyoshi , Saito Yatsumu , Matsumoto Kei , Takase Kentaro , Fukunaga Shohei , Okano Hirotaka James , Yokoo Takashi 
Published: May 2019

AbstractKidney regeneration is expected to be a new alternative treatment to the currently limited treatments for chronic kidney disease. By transplanting exogeneous nephron progenitor cells (NPCs) into the metanephric mesenchyme of a xenogeneic foetus, we aimed to regenerate neo-kidneys that originate from transplanted NPCs. Previously, we generated a transgenic mouse model enabling drug-induced ablation of NPCs (the Six2-iDTR mouse). We demonstrated that eliminating existing native host NPCs allowed their 100% replacement with donor mouse or rat NPCs, which could generate neo-nephrons on a culture dish. To apply this method to humans in the future, we examined the possibility of the in vivo regeneration of nephrons between different species via NPC replacement. We injected NPCs-containing rat renal progenitor cells and diphtheria toxin below the renal capsule of E13.5 metanephroi (MNs) of Six2-iDTR mice; the injected MNs were then transplanted into recipient rats treated with immunosuppressants. Consequently, we successfully regenerated rat/mouse chimeric kidneys in recipient rats receiving the optimal immunosuppressive therapy. We revealed a functional connection between the neo-glomeruli and host vessels and proper neo-glomeruli filtration. In conclusion, we successfully regenerated interspecies kidneys in vivo that acquired a vascular system. This novel strategy may represent an effective method for human kidney regeneration.
Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography
Authors: Lee Donghyeon , Baek Sangwon , Park Jaehyun , Lee Keondo , Kim Jangwoo , Lee Sang Jae , Chung Wan Kyun , Lee Jong-Lam , Cho Yunje , Nam Ki Hyun 
Published: May 2019

AbstractFixed-target serial femtosecond crystallography (FT-SFX) was an important advance in crystallography by dramatically reducing sample consumption, while maintaining the benefits of SFX for obtaining crystal structures at room temperature without radiation damage. Despite a number of advantages, preparation of a sample holder for the sample delivery in FT-SFX with the use of many crystals in a single mount at ambient temperature is challenging as it can be complicated and costly, and thus, development of an efficient sample holder is essential. In this study, we introduced a nylon mesh-based sample holder enclosed by a polyimide film. This sample holder can be rapidly manufactured using a commercially available nylon mesh with pores of a desired size at a low cost without challenging technology. Furthermore, this simple device is highly efficient in data acquisition. We performed FT-SFX using a nylon mesh-based sample holder and collected over 130,000 images on a single sample holder using a 30 Hz X-ray pulse for 1.2 h. We determined the crystal structures of lysozyme and glucose isomerase using the nylon mesh at 1.65 and 1.75 Å, respectively. The nylon mesh exposed to X-rays produced very low levels of background scattering at 3.75 and 4.30 Å, which are negligible for data analysis. Our method provides a simple and rapid but highly efficient way to deliver samples for FT-SFX.
Structural basis of substrate recognition by a novel thermostable (S)-enantioselective ω-transaminase from Thermomicrobium roseum
Authors: Kwon Sunghark , Lee Jun Hyuck , Kim Chang Min , Jang Hyunseok , Yun Hyungdon , Jeon Ju-Hong , So Insuk , Park Hyun Ho 
Published: May 2019

AbstractTransaminases catalyze the reversible transfer reaction of an amino group between a primary amine and an α-keto acid, utilizing pyridoxal 5′-phosphate as a cofactor. ω-transaminases (ωTAs) recognize an amino group linked to a non-α carbon of amine substrates. Recently, a novel (S)-enantioselective ωTA fromThermomicrobium roseum(Tr-ωTA) was identified and its enzymatic activity reported. However, the detailed mechanism of (S)-enantioselective substrate recognition remained unclear. In this study, we determined the crystal structure of Tr-ωTA at 1.8 Å resolution to elucidate the mechanism underlying Tr-ωTA substrate (S)-enantioselectivity. A structural analysis of Tr-ωTA along with molecular docking simulations revealed that two pockets at the active site tightly restrict the size and orientation of functional groups of substrate candidates. Based on the structural information and docking simulation results, we propose a comprehensive catalytic mechanism of Tr-ωTA. The present study thus provides structural and functional insights into the (S)-enantioselectivity of Tr-ωTA.
Bedrock sculpting under an active alpine glacier revealed from cosmic-ray muon radiography
Authors: Nishiyama R. , Ariga A. , Ariga T. , Lechmann A. , Mair D. , Pistillo C. , Scampoli P. , Valla P. G. , Vladymyrov M. , Ereditato A. , Schlunegger F. 
Published: May 2019

AbstractMountain glaciers form landscapes with U-shaped valleys, roche moutonées and overdeepenings through bedrock erosion. However, little evidence for active glacial carving has been provided particularly for areas above the Equilibrium Line Altitude (ELA) where glaciers originate. This is mainly due to our lack of information about the shape of the bedrock underneath active glaciers in highly elevated areas. In the past years, the bedrock morphology underneath active glaciers has been studied by geophysical methods in order to infer the subglacial mechanisms of bedrock erosion. However, these comprise surveys on the glaciers’ surface, from where it has been difficult to investigate the lateral boundary between the ice and the bedrock with sufficient resolution. Here we perform a muon-radiographic inspection of the Eiger glacier (Switzerland, European Alps) with the aid of cosmic-ray muon attenuation. We find a reach (600 × 300 m) within the accumulation area where strong lateral glacial erosion has cut nearly vertically into the underlying bedrock. This suggests that the Eiger glacier has profoundly sculpted its bedrock in its accumulation area. This also reveals that the cosmic-ray muon radiography is an ideal technology to reconstruct the shape of the bedrock underneath an active glacier.
LncRNA-H19 promotes hepatic lipogenesis by directly regulating miR-130a/PPARγ axis in non-alcoholic fatty liver disease
Authors: Liu Jun , Tang Tao , Wang Guo-Dong , Liu Bo 
Published: May 2019

Abstract
Background: As one of the most common liver disorders worldwide, non-alcoholic fatty liver disease (NAFLD) begins with the abnormal accumulation of triglyceride (TG) in the liver. Long non-coding RNA-H19 was reported to modulate hepatic metabolic homeostasis in NAFLD. However, its molecular mechanism of NAFLD was not fully clear.
Methods: In vitro and in vivo models of NAFLD were established by free fatty acid (FFA) treatment of hepatocytes and high-fat feeding mice, respectively. Hematoxylin and Eosin (H&E) and Oil-Red O staining detected liver tissue morphology and lipid accumulation. Immunohistochemistry (IHC) staining examined peroxisome proliferator-activated receptor γ (PPARγ) level in liver tissues. ELISA assay assessed TG secretion. Luciferase assay and RNA pull down were used to validate regulatory mechanism among H19, miR-130a and PPARγ. The gene expression in hepatocytes and liver tissues was detected by quantitative real-time PCR (qRT-PCR) and Western blotting.
Results: H19 and PPARγ were up-regulated, while miR-130a was down-regulated in NAFLD mouse and cellular model. H&E and Oil-Red O staining indicated an increased lipid accumulation. Knockdown of H19 inhibited steatosis and TG secretion in FFA-induced hepatocytes. H19 could bind to miR-130a, and miR-130a could directly inhibit PPARγ expression. Meanwhile, miR-130a inhibited lipid accumulation by down-regulating NAFLD-related genes PPARγ, SREBP1, SCD1, ACC1 and FASN. Overexpression of miR-130a and PPARγ antagonist GW9662 inhibited lipogenesis and TG secretion, and PPARγ agonist GW1929 reversed this change induced by miR-130a up-regulation.
Conclusion: Knockdown of H19 alleviated hepatic lipogenesis via directly regulating miR-130a/PPARγ axis, which is a novel mechanistic role of H19 in the regulation of NAFLD.
Circulating microRNA-378 levels serve as a novel biomarker for assessing the severity of coronary stenosis in patients with coronary artery disease
Authors: Li Hongshi , Gao Fei , Wang Xiaowei , Wu Jiahong , Lu Kunze , Liu Minghao , Li Rongrong , Ding Lingling , Wang Rong 
Published: May 2019

Abstract
Background: Circulating microRNAs (miRNA) are steady preserved in blood plasma. Multiple evidences have shown that miRNAs play a crucial role in cardiovascular disease including miRNA-378, which has been illustrated to participate in diverse physiological and pathological processes of cardiovascular disease. In the present study, we aim to explore the expression of plasma miRNA-378 and its clinical significance in patients with coronary artery disease (CAD).
Methods: MiRNA-378 expression in blood plasma was performed by quantitative real-time PCR (qRT-PCR) in 215 CAD patients and 52 matched controls of healthy populations. Medical information of all patients including the results of coronary angiography (CAG) was acquired through hospital information system (HIS). Spearman’s correlation, binary linear regression, and covariance analysis were used to examine the association between miRNA-378 and relative clinical risk factors. Receiver operating characteristic curve analysis was applied to evaluate the value of miRNA-378 in predicting the disease severity of coronary lesion.
Results: Plasma miR-378 expression was significantly down-regulated in CAD patients compared with healthy controls. Relative miR-378 level was shown conversely correlated with Gensini score, which present the severity of coronary artery lesions. Moreover, it is indicated that miR-378 expression can effectively distinguish patients with or without coronary artery stenosis.
Conclusions: Plasma miR-378 levels appear to be a promising non-invasive biomarker, but require to be further validated by a large cohort study in future.
Endoglin is a conserved regulator of vasculogenesis in zebrafish – implications for hereditary haemorrhagic telangiectasia
Authors: Zhang Ding , Zhou Fang , Zhao Xiuli , Liu Bao , Chen Jingyu , Yang Jun 
Published: May 2019

Abstract
Hereditary haemorrhagic telangiectasia (HHT) is a progressive vascular disease with high mortality and prevalence. There is no effective treatment of HHT due to the lack of comprehensive knowledge of its underlying pathological mechanisms. The majority of HHT1 patients carry endoglin (ENG) mutations. Here, we used Danio rerio (zebrafish) as an in vivo model to investigate the effects of endoglin knockdown on vascular development. According to phylogenetic analyses and amino acid sequence similarity analyses, we confirmed that endoglin is conserved in vertebrates and descended from a single common ancestor. Endoglin is highly expressed in the vasculature beginning at the segmentation period in zebrafish. Upon endoglin knockdown by morpholinos, we observed disruption in the intersegmental vessels (ISVs) and decreased expression of several vascular markers. RNA sequencing (RNA-Seq) results implied that the BMP-binding endothelial regulator (bmper) is a gene affected by endoglin knockdown. Rescue experiments demonstrated that overexpression of bmper significantly increased the number of endothelial cells (ECs) and reduced the defects at ISVs in zebrafish. Moreover, there was enhanced tube formation in ENG mutant ECs derived from a HHT patient after human recombinant BMPER (hrBMPER) stimulation. Taken together, our results suggest that bmper, a potential downstream gene of ENG, could be targeted to improve vascular integrity in HHT.
Long noncoding RNA HOXA-AS2 promotes non-small cell lung cancer progression by regulating miR-520a-3p
Authors: Liu Yunpeng , Lin Xingyu , Zhou Shiyao , Zhang Peng , Shao Guoguang , Yang Zhiguang 
Published: May 2019

Abstract
Background: The HOXA cluster antisense RNA 2 (HOXA-AS2) has recently been discovered to be involved in carcinogenesis in multiple cancers. However, the role and underlying mechanism of HOXA-AS2 in non-small cell lung cancer (NSCLC) yet need to be unraveled. Methods: HOXA-AS2 expression in NSCLC tissues and cell lines was detected using quantitative real-time PCR (qRT-PCR). Furthermore, the effects of HOXA-AS2 on NSCLC cell proliferation, apoptosis, migration, and invasion were assessed by MTS, flow cytometry, wound healing and transwell invasion assays, respectively. Starbase2.0 predicted and luciferase reporter and RNA immunoprecipitation (RIP) assays were used to validate the association of HOXA-AS2 and miR-520a-3p in NSCLC cells. Results: Our results revealed that HOXA-AS2 in NSCLC tissues were up-regulated and cell lines, and were associated with poor prognosis and overall survival. Further functional assays demonstrated that HOXA-AS2 knockdown significantly inhibited NSCLC cell proliferation, induced cell apoptosis and suppressed migration and invasion. Starbase2.0 predicted that HOXA-AS2 sponge miR-520a-3p at 3′-UTR, which was confirmed using luciferase reporter and RIP assays. miR-520a-3p expression was inversely correlated with HOXA-AS2 expression in NSCLC tissues. In addition, miR-520a-3p inhibitor attenuated the inhibitory effect of HOXD-AS2-depletion on cell proliferation, migration and invasion of NSCLC cells. Moreover, HOXA-AS2 could regulate HOXD8 and MAP3K2 expression, two known targets of miR-520a-3p in NSCLC. Conclusion: These findings implied that HOXA-AS2 promoted NSCLC progression by regulating miR-520a-3p, suggesting that HOXA-AS2 could serve as a therapeutic target for NSCLC.
Histological long-term effects in relevant tissues after 131I contamination of two Wistar rat models
Authors: Mezaguer-Lekouaghet Meriem , Badreddine Abdelwahab , Mameri Saadia , Souidi Maamar , Baz Ahcène , Lounis-Mokrani Zohra 
Published: May 2019

Procedures using iodine-131 (131I) represent about 90% of all therapies in nuclear medicine [1, 2]. It is important to evaluate the long-term biological effects of 131I treatment on non-target organs in order to improve the patient radioprotection.
The aim of this work is to use an experimental animal model to contribute to the understanding of some biological effects induced by 131I contamination, and especially histological effects.
Two models of Wistar rats with (Tr+) and without thyroid (Tr-) were orally contaminated with iodine and after 8 months they were sacrificed and the histological effects on some relevant tissues such as thyroid, testes, liver and kidneys were been studied.
Thyroid tissue analysis revealed that in the case of the Tr+ model compared to their control (Tr+ uncontaminated), 50% of the slides examined had remodeled the thyroid tissue with rare follicles choked by fibrosis and with epithelial changes. However, for Tr- compared to their control, the examined slides reveal the presence of a small, completely atrophied thyroid mass associated with vesicular fibrosis and with detachment of the colloid.
For the renal organ, disturbances are observed: inflammation of the presence of tissue fibrosis and glomerular necrosis. For the liver, there is an appearance of inflammatory focus in different degrees around the portals. However, the results of the testes of both models compared to their controls revealed no histological abnormalities. The observed histological effects are correlated with the corresponding absorbed dose received by each organ and calculated using the RODES software [3, 4].
Possible role of auroral oval-related currents in two intense magnetic storms recorded by old mid-latitude observatories Clementinum and Greenwich
Authors: Valach Fridrich , Hejda Pavel , Revallo Miloš , Bochníček Josef 
Published: May 2019

Some recent studies point out that currents related to the auroral oval, electrojets and field aligned currents (FACs), are serious candidates for the mechanism of the intense mid-latitude magnetic storms. It is interesting to re-analyse historical data under the light of this modern knowledge. In this aim, we analysed two intense magnetic storms that were recorded by observatories Clementinum (Prague) and Greenwich on 17 November 1848 and 4 February 1872, respectively. The latter has been marked as an extraordinary event by several authors, in particular in connection with auroras. The former, however, has been little known in the space weather community. Both these events possessed swift and extensive variations of the horizontal (H) component (>400 nT and >500 nT, respectively) and were accompanied by auroras sighted at very low magnetic latitudes. This implies that the auroral oval on the north hemisphere was vastly extended southward. The variations of the magnetic declination also indicate that during these events the auroral oval was situated at magnetic latitudes lower than those of the observatories. The storms studied in this paper occurred at different magnetic local times (MLTs), ~23 MLT and ~19 MLT. Therefore, they might represent mid-latitude events related to different parts of the auroral oval. In this paper, theH-variation recorded at Clementinum in 1848 is interpreted to be a substorm due to the ionospheric substorm electrojet. The Greenwich event registered in 1872 then seems to be a combination of the ring-current storm with a positive variation of theH-component caused by the eastward electrojet. Both the events of 1848 and 1872 appear to exemplify phenomena that are common in high magnetic latitudes but which may occasionally happen also at mid-latitudes.
[99mTc]Tilmanocept Lymphscintigraphy after inconclusive [99mTc]Nanocolloid Scan in Breast Cancer
Authors: Kircher Malte , Seifert Simone , Kircher Stefan , Lapa Constantin 
Published: May 2019

IntroductionLymphatic mapping for identification and subsequent removal of the sentinel lymph node (SLN) is an established procedure in breast cancer and cutaneous melanoma to minimize the extent of surgery (and thus, associated morbidity), simplify histopathological processing and subsequently provide prognostic information and help choose the optimal patient management. Established methods for SLN mapping include visual identification of nodal staining after peritumoral injection of a (blue) dye or the use of lymphoscintigraphy with technetium-labelled nanocolloid. In experienced hands, success rates for both methods exceed 95 %, nonetheless in some patients they fail despite correct application and imaging techniques. Potential reasons for false-negative SLN detection rates –beyond poor tracer injection technique or imaging of the wrong nodal basin- include inadequate pathologic examination of the SLN or complete replacement of the SLN with neoplastic disease, causing the injected tracer to completely bypass the infiltrated node 1.Beyond colloid particles, the more specific receptor-targeting small molecule [99mTc]Tilmanocept has recently been approved for scintigraphic SLN detection. Tilmanocept, or mannosyl diethylene-triamine-pentaacetate (DTPA) dextran, has a small molecular size of approximately 7 nm and works via specific binding to the mannose receptor (CD206) 2. The mannose receptor is particularly overexpressed on macrophages and dendritic precursor cells within lymph nodes, thus uptake in lymph nodes is not dependent on particle size 2, 3. In pilot studies scintigraphic SLN detection with [99mTc]Tilmanocept was superior to dye staining 4. Given its beneficial properties, [99mTc]Tilmanocept might offer advantages over the alternatively used radiocolloids. We present four cases of [99mTc]Tilmanocept application after inconclusive or unsuccessful attempts of SLN detection using [99mTc]nanocolloid lymphoscintigraphy.
Cp*CoIII-Catalyzed C–H Functionalization and Asymmetric Reactions Using External Chiral Sources
Authors: Yoshino Tatsuhiko , Matsunaga Shigeki 
Published: May 2019

This account describes Cp*CoIII-catalyzed C–H functionalization reactions developed in our group between 2013 and 2018. Cp*CoIII catalysts not only serve as inexpensive alternatives to Cp*RhIII catalysts but also exhibit unique reactivity and selectivity in several transformations. In the latter part of this review, we introduce catalytic asymmetric C–H functionalization reactions using achiral RhIII or CoIII catalysts with chiral disulfonates or carboxylic acids as external chiral sources.1	Introduction and Overview2	Cp*CoIII-Catalyzed C–H Functionalization Reactions2.1	C–H Addition Reactions to Polar Double Bonds2.2	Cp*Co(CO)I2 and [Cp*CoI2]2 Precursors for the C2-selective C–H Amidation of Indoles2.3	C–H Functionalization of Carbamoyl-Protected Indoles Using Alkynes2.4	C–H Allylation Using Allyl Alcohols2.5	Cyclization Reactions of O-Acyloximes and Alkynes2.6	Other Miscellaneous Reactions3	Enantioselective C–H Functionalization Reactions by Hybrid Catalysis3.1	Cp*RhIII/Chiral Disulfonate Catalysts for the Enantioselective C–H Addition to Enones3.2	Enantioselective C–H Cleavage Using Chiral Carboxylic Acids4	Summary and Perspective