bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of tendon to produce decellularized sheets of mineralized collagen fibrils for bone tissue repair and regeneration
Authors: Grue Brendan H. , Veres Samuel P. 
Published: June 2019

AbstractWith demand for alternatives to autograft and allograft materials continuing to rise, development of new scaffolds for bone tissue repair and regeneration remains of significant interest. Engineered collagen‐calcium phosphate (CaP) constructs can offer desirable attributes, including absence of foreign body response and possession of inherent osteogenic potential. Despite their promise, current collagen‐CaP constructs are limited to nonload‐bearing applications. In this article, we describe a process for creating decellularized sheets of highly aligned, natively cross‐linked, and mineralized collagen fibrils, which may be useful for developing multilaminate collagen‐CaP constructs with improved mechanical properties. Decellularized bovine tendons were cryosectioned to produce thin sheets of aligned collagen fibrils. Mineralization of the sheets was then performed using an alternate soaking method incorporating a polymer‐induced liquid precursor (PILP) process to promote intrafibrillar mineralization, along with incorporation of physiologically relevant amounts of citrate, Mg, and carbonate. Characteristics of the produced scaffolds were assessed using energy‐dispersive X‐ray spectroscopy (EDX), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Scaffolds were also compared with both native bovine cortical bone and pure hydroxyapatite using X‐ray powder diffraction (XRD), and Fourier transform infrared spectroscopy attenuated total reflection (FTIR‐ATR). Structural and chemical analyses show that the scaffold preparation process that we described is successful in creating mineralized collagen sheets, possessing a mineral phase similar to that found in bone as well as a close association between collagen fibrils and mineral plates.
Patient‐centered care and genomic medicine: A qualitative provider study in the military health system
Authors: Hellwig Lydia D. , Turner Clesson , O’Neill Suzanne C. 
Published: June 2019

AbstractThe diagnostic and predictive information produced by genomic sequencing may impact medical management, and it is critical that providers and institutions are able to use this information appropriately for patient care. Guided by the patient‐centered care model, we investigated provider perspectives of patient, provider, and system‐level factors that could influence the implementation of genomic medicine within the integrated healthcare system of the US Department of Defense (DOD). The purpose of this study was to explore patient‐centered care elements related to the application of genomic sequencing in a military healthcare facility to understand the current capability and key gaps for patient‐centered genomic medicine. Twenty DOD healthcare providers were interviewed regarding their past experiences and future expectations of genetics and genomics. These semi‐structured interviews were recorded, transcribed and analyzed. All providers interviewed had some experience with genetics, but the level of experience varied greatly. Providers reported widely differing degrees of knowledge and confidence regarding genetics and about military‐specific policies regarding genetics which varied by specialty. In addition, most providers stated that their department did not currently have the infrastructure to allow for the care of patients with secondary genetic findings, defined as genetic findings which are intentionally examined because of their importance to healthcare management, but are unrelated to the reason the individual underwent sequencing. This study reveals gaps in key elements of patient‐centered care related to genomic medicine that may be helpful to address in future implementation efforts.
Nucleic acid and SNP detection via template‐directed native chemical ligation and inductively coupled plasma mass spectrometry
Authors: Lores Lareo Pablo , Linscheid Michael W. , Seitz Oliver 
Published: June 2019

AbstractDetection of nucleic acids and single nucleotide polymorphisms (SNPs) is of pivotal importance in biology and medicine. Given that the biological effect of SNPs often is enhanced in combination with other SNPs, multiplexed SNP detection is desirable. We show proof of concept of the multiplexed detection of SNPs based on the template‐directed native chemical ligation (NCL) of PNA‐probes carrying a metal tag allowing detection using ICP‐MS. For the detection of ssDNA oligonucleotides (30 bases), two probes, one carrying the metal tag and a second one carrying biotin for purification, are covalently ligated. The methodological limit of detection is of 29 pM with RSD of 6.7% at 50 pM (n = 5). Detection of SNPs is performed with the combination of two sets of reporter probes. The first probe set targets the SNP, and its yield is compared with a second set of probes targeting a neighboring sequence. The assay was used to simultaneously differentiate between alleles of three SNPs at 5‐nM concentration.
Multi‐century trends to wetter winters and drier summers in the England and Wales precipitation series explained by observational and sampling bias in early records
Authors: Murphy Conor , Wilby Robert L. , Matthews Tom K. R. , Thorne Peter , Broderick Ciaran , Fealy Rowan , Hall Julia , Harrigan Shaun , Jones Phil , McCarthy Gerard , MacDonald Neil , Noone Simon , Ryan Ciara 
Published: June 2019

AbstractGlobally, few precipitation records extend to the 18th century. The England Wales Precipitation (EWP) series is a notable exception with continuous monthly records from 1766. EWP has found widespread use across diverse fields of research including trend detection, evaluation of climate model simulations, as a proxy for mid‐latitude atmospheric circulation, a predictor in long‐term European gridded precipitation data sets, the assessment of drought and extremes, tree‐ring reconstructions and as a benchmark for other regional series. A key finding from EWP has been the multi‐centennial trends towards wetter winters and drier summers. We statistically reconstruct seasonal EWP using independent, quality‐assured temperature, pressure and circulation indices. Using a sleet and snow series for the UK derived by Profs. Gordon Manley and Elizabeth Shaw to examine winter reconstructions, we show that precipitation totals for pre‐1870 winters are likely biased low due to gauge under‐catch of snowfall and a higher incidence of snowfall during this period. When these factors are accounted for in our reconstructions, the observed trend to wetter winters in EWP is no longer evident. For summer, we find that pre‐1820 precipitation totals are too high, likely due to decreasing network density and less certain data at key stations. A significant trend to drier summers is not robustly present in our reconstructions of the EWP series. While our findings are more certain for winter than summer, we highlight (a) that extreme caution should be exercised when using EWP to make inferences about multi‐centennial trends, and; (b) that assessments of 18th and 19th Century winter precipitation should be aware of potential snow biases in early records. Our findings underline the importance of continual re‐appraisal of established long‐term climate data sets as new evidence becomes available. It is also likely that the identified biases in winter EWP have distorted many other long‐term European precipitation series.
Consumer motivation for product disposal and its role in acquiring products for reuse
Authors: Simpson Dayna , Power Damien , Riach Kathleen , Tsarenko Yelena 
Published: June 2019

AbstractProduct reuse is most profitable where manufacturers acquire used products in the best possible condition—near new and with little wear and tear. This requires consumers, however, to dispose of products that still work and may still be in use. Prior scholarship on acquiring consumer products for reuse focuses on consumer “returns”—products consumers find fault with, or bought in error. These, however, are a fraction of all products sold to consumers and available for reuse. Importantly, returns are motivated by a different set of factors, than product “disposal.” We explore how psychological ownership influences consumer disposal of reusable products. Across three studies of Australian consumers, we found two psychological tendencies (attachment and frugality) increased product retention. We also found, that infrequent product use, and emotional reward, could weaken ownership and encourage disposal of products that are attractive for reuse. To our knowledge, no behavioral studies in the product acquisition literature deal with consumers before or during product disposal. We highlight a role for consumers in product acquisition, as well as contribute to the consumer psychology and mental accounting literature, and identify significant opportunity for manufacturers to leverage consumers' psychological tendencies to improve the collection of reusable products.
A 5291‐ppi organic light‐emitting diode display using field‐effect transistors including a c‐axis aligned crystalline oxide semiconductor
Authors: Katsui Shuichi , Kobayashi Hidetomo , Nakagawa Takashi , Tamatsukuri Yuki , Shishido Hideaki , Uesaka Shogo , Yamaoka Ryohei , Nagata Takaaki , Aoyama Tomoya , Nei Kosei , Okazaki Yutaka , Ikeda Takayuki , Yamazaki Shunpei 
Published: June 2019

AbstractC‐axis‐aligned crystalline‐oxide semiconductor field‐effect transistor (CAAC‐OS FET) can be scaled down to a width and a length of 60 nm. We fabricated an organic light‐emitting diode (OLED) display with more than 5000 ppi, which is required in virtual reality (VR) display applications, using CAAC‐OS FETs as the backplane.
Examining medical tourists' intention to visit a tourist destination: Application of an extended MEDTOUR scale in a cosmetic tourism context
Authors: Liang Lena Jingen , Choi Hwansuk Chris , Joppe Marion , Lee Woojin 
Published: June 2019

AbstractBehavioral studies of medical tourists are still limited despite a recent and rapid boom in both the business and academic fields. In 2011, Martin, Ramamonjiarivelo, and Martin proposed their MEDTOUR scale to better understand medical tourists' intention to seek treatment overseas. However, the scale has not been validated through application in a different context to date. The present study aims to fill this gap by examining its reliability and applying it in an extended model with perceived risk and perceived benefit. Based on the results of the data collected from Chinese adults, the MEDTOUR scale achieved an acceptable level of factorial, convergent, and discriminant validity. Support was confirmed for all hypotheses with a relatively weak relation between perceived risk and attitude, as well as perceived behavioral control and behavior intention. This study's findings fully support the prediction of behavior intention to travel to a foreign country for medical treatment and provides some useful findings to help medical tourism marketers and hospitals in developing their strategies.
Amino Acid–Substituted Dextran‐Based Non‐Viral Vectors for Gene Delivery
Authors: Zink Matthias , Hotzel Konrad , Schubert Ulrich S. , Heinze Thomas , Fischer Dagmar 
Published: June 2019

AbstractTo form bio‐inspired non‐viral vectors for DNA delivery, the polysaccharide dextran is allowed to react with Boc‐amino protected amino acids glycine, β‐alanine, and L‐lysine activated with 1,1’‐carbonyldiimidazole and subsequent dextran ester deprotection. A library of such dextran esters is made available to investigate the relationship between polymer structure, complex formation, stability, toxicity, and transfection. Only dextran esters of β‐alanine and L‐lysine are able to efficiently interact with DNA as shown by dye exclusion assays, to form nanosized complexes (70–110 nm) with positive zeta potential. With increasing substitution degree and complex charge ratios, the L‐lysine esters accomplish more effective binding and protection of DNA against enzymatic degradation than β‐alanine esters. However, luciferase reporter gene assays reveal higher transfection for β‐alanine than for L‐lysine esters due to a more effective DNA release and better suited buffing area of the amino groups triggering the endosomal release. Conclusively, β‐alanine‐substituted dextran derivatives may serve as promising non‐viral vectors.
The role of FOXO3 polymorphisms in susceptibility to tuberculosis in a Chinese population
Authors: Wang Bo , Wang Yuhe , Wang Li , He Xue , He Yongjun , Bai Mei , Zhu Linhao , Zheng Jianwen , Yuan Dongya , Jin Tianbo 
Published: June 2019

AbstractBackgroundTuberculosis (TB) is a significant worldwide health problem, and is caused by Mycobacteria tuberculosis. Recent studies have suggested that FOXO3 plays vital roles in the risk of immune–related infectious diseases such as TB.Methods and ResultsThe present study aimed to evaluate FOXO3 genetic variants and TB risk. We recruited 510 TB patients and 508 healthy controls in this study. All subjects were genotyped with the Agena MassARRAY platform. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using logistic regression adjusted for age and gender. Our result revealed that rs3800229 T/G and rs4946935 G/A genotypes significantly increased the risk of TB (OR = 1.34, 95% CI = 1.04–1.74, p = 0.026; OR = 1.34, 95% CI = 1.03–1.73, p = 0.029, respectively). In stratified analysis according to gender and age, we observed that rs3800229 T/G and rs4946935 G/A genotypes were associated with an increase the risk of TB among males and age ≤41 years, respectively (OR = 1.47, 95% CI = 1.06–2.04, p = 0.022 and OR = 1.45, 95% CI = 1.05–2.02, p = 0.025).ConclusionsOur study showed that rs3800229 and rs4946935 in FOXO3 were associated with a risk of TB in the Chinese population.
How to deal with consumers who group to request a discount?
Authors: Qiu Qijun , Jiang Li 
Published: June 2019

AbstractWe investigate operations impacts of consumer‐initiated group buying (CGB), whereby consumers voluntarily form buying groups to negotiate bulk deals with retailers. This differs from regular purchasing whereby consumers visit retailers individually and pay posted prices. Upon the visit by group consumers, a retailer decides to forgo or satisfy their demand in its entirety. Turned down by a retailer, group consumers continue to visit other retailers. In the case where their group effort fails to conclude a deal, some group consumers switch to individual purchasing provided they receive a non‐negative utility by doing so. Even after a successful group event, the group consumers who forgo the event out of utility concern may switch to individual purchasing as well. Retailer competition, group size, and the chance that group consumers switch to individual purchasing upon unsatisfaction are crucial to how retailers adjust operations to deal with CGB. With retailer competition, the rise of CGB results in every consumer paying the same reduced price when group size is small but makes group consumers pay more than by purchasing individually when group size is large. This has mixed consequences on the profits for retailers in both absolute and relative terms.
Lexicographically optimal earliest arrival flows
Authors: Kamiyama Naoyuki 
Published: June 2019

AbstractA dynamic network introduced by Ford and Fulkerson is a directed graph in which each arc has a capacity and a transit time. The evacuation problem is one of the fundamental problems in a dynamic network. The goal of this problem is to find the minimum time limit Θ such that we can send all the supplies to the sinks within time Θ. An earliest arrival flow is an optimal flow for the evacuation problem such that the amount of supplies which have reached the sinks is maximized at every time step. It is known that in a dynamic network with multiple sinks, if the sinks have capacities, then an earliest arrival flow does not necessarily exist. In this paper, to cope with this issue, we first introduce a lexicographically optimal earliest arrival flow in a dynamic network with multiple sinks. Then we propose a pseudo‐polynomial‐time algorithm for finding a lexicographically optimal earliest arrival flow. Furthermore, we prove that if the transit time of every arc is zero, then we can find a lexicographically optimal earliest arrival flow in polynomial time.
Effectiveness of high‐frequency left prefrontal repetitive transcranial magnetic stimulation in patients with treatment‐resistant depression: A randomized clinical trial of 37.5‐minute vs 18.75‐minute protocol
Authors: Kito Shinsuke , Miyazi Midori , Nakatani Honoka , Matsuda Yuki , Yamazaki Ryuichi , Okamoto Tatsuya , Igarashi Yoshio 
Published: June 2019

AbstractAimClinical trials and meta‐analyses have demonstrated the efficacy of high‐frequency repetitive transcranial magnetic stimulation (rTMS) over the left prefrontal cortex in treatment‐resistant depression. The aim of this study was to prospectively evaluate the effectiveness of the conventional 37.5‐minute vs 18.75‐minute rTMS protocol over the left prefrontal cortex in patients with treatment‐resistant depressive episode.MethodsThirty patients with treatment‐resistant depression or bipolar disorder depressive episode were randomized 1:1 to either 37.5‐minute or 18.75‐minute rTMS protocol groups. rTMS treatment was applied at 120% resting motor threshold with 10 Hz over the left prefrontal cortex. Treatment sessions were delivered for a total of 3000 pulses/d, 5 days a week, for 4‐6 weeks. Patients received a 75 trains with “4 sec on and 26 sec off” for 37.5 minutes or a 75 trains with “4 sec on and 11 sec off” for 18.75 minutes. Severity of depression was rated with the Quick Inventory of Depressive Symptomatology (QIDS) and Patient Health Questionnaire (PHQ‐9). Remission was defined as a total score of 5 or less on the QIDS. The primary outcome measure was to compare the remission rate between the both groups.ResultsThirteen of 30 patients (43.3%) showed remission at week 6. There were no significant differences in the remission rate between the conventional 37.5‐ and 18.75‐minute protocol groups (46.7% and 40.0%, respectively). No seizures or treatment‐emergent mania/hypomania were occurred.ConclusionThese findings suggest that, compared with the conventional one, rTMS with 18.75‐minute protocol might be equally effective and clinically beneficial in saving the treatment session length. Further well‐designed studies are needed.
Short‐term impact of breast cancer screening intervention on health‐related quality of life in China: A multicentre cross‐sectional survey
Authors: Zhu Juan , Wang Le , Huang Hui‐Yao , Bai Fang‐Zhou , Li Jing , Fang Yi , Wang Yong , Mao A‐Yan , Liao Xian‐Zhen , Liu Guo‐Xiang , Bai Ya‐Na , Ren Jian‐Song , Sun Xiao‐Jie , Guo Lan‐Wei , Wang De‐Bin , Dong Heng‐Jin , Wang Jia‐Lin , Xing Xiao‐Jing , Zhou Qi , Zhu Lin , Du Ling‐Bin , Song Bing‐Bing , Luo Su‐Xia , Liu Yu‐Qin , Ren Ying , Zhou Jin‐Yi , Sun Xiao‐Hua , Lan Li , Wu Shou‐Ling , Qi Xiao , Lou Pei‐An , Ma Fei , Li Ni , Zhang Kai , Chen Wan‐Qing , He Jie , Dai Min , Shi Ju‐Fang 
Published: June 2019

AbstractObjectiveThe impact of participating in breast cancer screening programmes on health‐related quality of life (HRQoL)is poorly understood.MethodsBased on a national breast cancer screening programme in China, a multicentre cross‐sectional survey was conducted covering 12 provinces from September 2013 to December 2014. HRQoL of participants in the screening population and general population was evaluated by the three‐levelEuroQol‐five‐Dimensions (EQ‐5D‐3L) instrument, and utility scores were generated through the Chinese value set. Univariate and multivariate regression analyses were performed to explore determinants of utility scores and anxiety/depression problems.ResultsFor screening group and general population (n = 4756, mean age = 51.6 year old), the corresponding utility scores were 0.937 (95% CI, 0.933‐0.941) and 0.953 (0.949‐0.957) (P < .001). Pain/discomfort and anxiety/depression were the most common reported in both groups (51.4% and 34.3%, P < .001). Utility scores at prescreening, in‐screening, and postscreening interview timings were 0.928 (0.921‐0.935), 0.958 (0.948‐0.969), and 0.938 (0.933‐0.943), respectively (P < .001); the corresponding proportions of anxiety/depression reporting were 25.9%, 16.3%, and 21.1%, respectively (P = .004). Interview timing, geographical region, and insurance status were associated with HRQoL and anxiety/depression in women at high‐risk of breast cancer.ConclusionsUtility scores of screening participants were significantly lower than that of general population in China, but the difference may be clinically insignificant. Further cohort studies using HRQoL measurements are needed.
A simplified method for detection of N‐terminal valine adducts in patients receiving treosulfan
Authors: Boysen Gunnar , Shimoni Avichai , Danylesko Ivetta , Varda‐Bloom Nira , Nagler Arnon 
Published: June 2019

RATIONALETreosulfan is a substance that is being studied as part of the conditioning regimen given prior to allogeneic stem cell transplantation in patients with hematological malignancies. It is known to decompose into 1,2:3,4‐diepoxybutane (DEB) under physiologic conditions. In this study, we investigate whether N‐terminal valine adducts can be utilized to monitor differences in DEB formation of patients receiving treosulfan as part of the conditioning regimen for transplantation.METHODSBlood samples were collected from a group of 14 transplant recipients and analyzed for N,N‐(2,3‐dihydroxy‐1,4‐butadiyl)valine (pyr‐Val) and 2,3,4‐trihydroxybutylvaline (THB‐Val) adducts as biomarkers for drug uptake and metabolism before treosulfan treatment and 6 days after treatment.RESULTSA new direct injection liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated prior to clinical analysis. The assay precision was determined by 3 replicate analyses on 3 individual days using control globin spiked with known amounts of pyr‐Val and THB‐Val. The intra‐ and inter‐day precision coefficients of variance (CVs) and accuracy were < 10% and 15%, respectively. In clinical specimens, the means ± SD of pyr‐Val and THB‐Val background were 0.29 ± 0.10 pmol/g HB and 5.17 ± 1.7 pmol/g HB, respectively.CONCLUSIONSThese values are similar to those found previously. Treosulfan treatment leads to a significant increase in pyr‐Val and THB‐Val adducts in each patient (Student's t‐test p <0.0001). The mean ± SD amounts of adduct formed were 245.3 ± 89.6 and 210 ± 78.5 pmol/g globin for pyr‐Val and THB‐Val, respectively. Importantly, these results show that this direct injection method can quantitate both background and treosulfan‐induced pyr‐Val and THB‐Val N‐terminal valine globin adducts in humans.