bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comprehensive proteomics-based interaction screen that links DYRK1A to RNF169 and to the DNA damage response
Authors: Roewenstrunk Julia , Di Vona Chiara , Chen Jie , Borras Eva , Dong Chao , Arató Krisztina , Sabidó Eduard , Huen Michael S. Y. , de la Luna Susana 
Published: April 2019

AbstractDysregulation of the DYRK1A protein kinase has been associated with human disease. On the one hand, its overexpression in trisomy 21 has been linked to certain pathological traits of Down syndrome, while on the other, inactivating mutations in just one allele are responsible for a distinct yet rare clinical syndrome, DYRK1A haploinsufficiency. Moreover, altered expression of this kinase may also provoke other human pathologies, including cancer and diabetes. Although a few DYRK1A substrates have been described, its upstream regulators and downstream targets are still poorly understood, an information that could shed light on the functions of DYRK1A in the cell. Here, we carried out a proteomic screen using antibody-based affinity purification coupled to mass spectrometry to identify proteins that directly or indirectly bind to endogenous DYRK1A. We show that the use of a cell line not expressing DYRK1A, generated by CRISPR/Cas9 technology, was needed in order to discriminate between true positives and non-specific interactions. Most of the proteins identified in the screen are novel candidate DYRK1A interactors linked to a variety of activities in the cell. The in-depth characterization of DYRK1A’s functional interaction with one of them, the E3 ubiquitin ligase RNF169, revealed a role for this kinase in the DNA damage response. We found that RNF169 is a DYRK1A substrate and we identified several of its phosphorylation sites. In particular, one of these sites appears to modify the ability of RNF169 to displace 53BP1 from sites of DNA damage. Indeed, DYRK1A depletion increases cell sensitivity to ionizing irradiation. Therefore, our unbiased proteomic screen has revealed a novel activity of DYRK1A, expanding the complex role of this kinase in controlling cell homeostasis.
Evidence for altered neurodevelopment and neurodegeneration in Wolfram syndrome using longitudinal morphometry
Authors: Lugar Heather M. , Koller Jonathan M. , Rutlin Jerrel , Eisenstein Sarah A. , Neyman Olga , Narayanan Anagha , Chen Ling , Shimony Joshua S. , Hershey Tamara 
Published: April 2019

AbstractWolfram syndrome is a rare disease caused by mutations in the WFS1 gene leading to symptoms in early to mid-childhood. Brain structural abnormalities are present even in young children, but it is not known when these abnormalities arise. Such information is critical in determining optimal outcome measures for clinical trials and in understanding the aberrant neurobiological processes in Wolfram syndrome. Using voxel-wise and regional longitudinal analyses, we compared brain volumes in Wolfram patients (n = 29; ages 5–25 at baseline; mean follow-up = 3.6 years), to age and sex-equivalent controls (n = 52; ages 6–26 at baseline; mean follow-up = 2.0 years). Between groups, white and gray matter volumes were affected differentially during development. Controls had uniformly increasing volume in white matter, whereas the Wolfram group had stable (optic radiations) or decreasing (brainstem, ventral pons) white matter volumes. In gray matter, controls had stable (thalamus, cerebellar cortex) or decreasing volumes (cortex), whereas the Wolfram group had decreased volume in thalamus and cerebellar cortex. These patterns suggest that there may be early, stalled white matter development in Wolfram syndrome, with additional degenerative processes in both white and gray matter. Ideally, animal models could be used to identify the underlying mechanisms and develop specific interventions.
Effects of Physical Activity During Pregnancy on Neonatal Birth Weight
Authors: Pathirathna Malshani L. , Sekijima Kayoko , Sadakata Mieko , Fujiwara Naoshi , Muramatsu Yoshiyuki , Wimalasiri Kuruppu M. S. 
Published: April 2019

AbstractWe assessed the prevalence of adherence to the American College of Obstetricians and Gynecologists (ACOG) recommendations regarding physical activity during pregnancy among Sri Lankan women and explored the relationship between physical activity during pregnancy and neonatal birth weight. In total, 141 pregnant women (gestational age, 18–24 weeks) were included from October to December 2015 and followed up until delivery. A validated questionnaire regarding physical activity during pregnancy was administered in the second and third trimesters. Activities were grouped by type (household/caregiving, occupational, transportation, sports/exercise, and inactivity) and intensity {sedentary [<1.5 metabolic equivalents (METs)], light intensity [1.5–2.9 METs], moderate intensity [3.0–6.0 METs], and vigorous intensity [>6.0 METs]}. Women were categorised as active or inactive based on the ACOG recommendations. In total, 79.1% and 45.2% of women met the guidelines in the second and third trimesters, respectively. The overall time spent and total energy expenditure was significantly higher in the second trimester (p < 0.001). We found no relationship between physical activity during pregnancy and neonatal birth weight. This study indicates that a considerable reduction of time and total energy expenditure occur as pregnancy progresses. Physical activity during pregnancy does not appear to significantly affect neonatal birth weight.
Impact of environmental factors on aquatic biodiversity in roadside stormwater ponds
Authors: Sun Zhenhua , Sokolova Ekaterina , Brittain John E. , Saltveit Svein Jakob , Rauch Sebastien , Meland Sondre 
Published: April 2019

AbstractConstructed stormwater ponds mitigate runoff volumes and pollution, and provide other ecosystem services, such as supporting biodiversity, but these services attracted relatively less attention. The impacts of the pollution levels in the water column and sediments, the physical characteristics of ponds, and the presence of amphibians on the macroinvertebrate community composition and biodiversity were explored in twelve stormwater ponds in Norway. Also, the similarities between macroinvertebrate, zooplankton and plant communities were explored. Most of the taxa displayed in the ordination diagram were positively correlated with the pond size and the number of neighbouring ponds, and negatively correlated with the pollution levels in the water column and sediments. However, no statistically significant impacts on the number of taxa and Shannon index were observed. There were low similarities between the macroinvertebrate and zooplankton community compositions as well as between the plant and macroinvertebrate community compositions in the stormwater ponds. We observed a significant positive correlation between the number of plant and of zooplankton taxa, and a weak non-significant positive correlation between the number of plant and of macroinvertebrate taxa. Overall, the explanatory variables had a significant impact on the community composition, but not on the number of taxa nor Shannon index.
Increased influence of ENSO on Antarctic temperature since the Industrial Era
Authors: Rahaman Waliur , Chatterjee Sourav , Ejaz Tariq , Thamban Meloth 
Published: April 2019

AbstractUnder the influence of recent global warming, modulation of frequencies and amplitude of El Niño-Southern Oscillation (ENSO) and its impacts on global climate have become great concerns to the global community. Antarctic climate is sensitive to these changes owing to tropical and Southern Hemispheric (SH) teleconnections. Antarctic surface air temperature (SAT) reconstructed approximately for the past five centuries (~1533 to 1993 CE) based on multiple oxygen isotope (δ18O) records of ice cores from East and West Antarctica show dominant oscillations in ENSO and Pacific Decadal Oscillation (PDO) frequency bands. Further, variance of the East Antarctica (EA) temperature record shows significant increasing trend at ENSO band and decreasing trend at PDO band since the industrial era (~1850 CE). This observation is consistent with the earlier report of increasing ENSO activity, reconstructed based on tropical-subtropical tree ring records. ENSO influence in the SH high-latitude is known to be characterized by Pacific South American (PSA) pattern reflected in the atmospheric pressure fields. Our investigation of greenhouse gas (GHG) forced model simulation results show an increasing trend in PSA activity since the industrial era. Thus, we suggest ENSO activity and its influence on Antarctic temperature are increasing in response to increasing radiative GHG forcing since the industrial era.
Relationship of High-Density Lipoprotein-Associated Arylesterase Activity to Systolic Heart Failure in Patients with and without Type 2 Diabetes
Authors: Li Chang , Chen Jia Wei , Ding Feng Hua , Shen Ying , Liu Zhu Hui , Wang Fang , Zhang Rui Yan , Shen Wei Feng , Lu Lin , Wang Xiao Qun 
Published: April 2019

AbstractHigh-density lipoprotein (HDL) confers protection against cardiovascular disease partly attributable to its robust anti-oxidant activities, which is largely impaired in diabetic conditions. In this study, we analyzed the anti-oxidant activity of HDL, as represented by the arylesterase activity of paraoxonase 1 (PON1) in HDL particles, in 216 consecutive HF patients with (n = 79) or without (n = 137) type 2 diabetes, and age- and gender-matched 112 diabetic and 189 non-diabetic non-HF controls. We found arylesterase activity was significantly decreased in patients with than without HF, and was further decreased when comorbid with diabetes. After adjusting for conventional risk factors and apolipoprotein A-I levels, arylesterase activity remained correlated positively with left ventricular ejection fraction in diabetic (r = 0.325, P = 0.020) but not non-diabetic patients (r = 0.089, P = 0.415), and negatively with NT-proBNP and NYHA functional class in both subgroups. In regression analyses, a higher risk of HF was observed in diabetic than non-diabetic patients when having low arylesterase activities. In conclusion, our data demonstrate that impaired serum arylesterase activity in patients with HF is further reduced when comorbid with diabetes. The relationship of impaired arylesterase activity to HF is especially enhanced in diabetic patients.
Robust and biocompatible catalysts for efficient hydrogen-driven microbial electrosynthesis
Authors: Kracke Frauke , Wong Andrew Barnabas , Maegaard Karen , Deutzmann Joerg S. , Hubert McKenzie A. , Hahn Christopher , Jaramillo Thomas F. , Spormann Alfred M. 
Published: April 2019

AbstractCO2 reduction by combined electro- and bio-catalytic reactions is a promising technology platform for sustainable production of chemicals from CO2 and electricity. While heterogeneous electrocatalysts can reduce CO2 to a variety of organic compounds at relatively high reaction rates, these catalysts have limitations achieving high selectivity for any single product beyond CO. Conversely, microbial CO2 reduction pathways proceed at high selectivity; however, the rates at bio-cathodes using direct electron supply via electricity are commonly limiting. Here we demonstrate the use of non-precious metal cathodes that produce hydrogen in situ to support microbial CO2 reduction to C1 and C2 compounds. CoP, MoS2 and NiMo cathodes perform durable hydrogen evolution under biologically relevant conditions, and the integrated system achieves coulombic efficiencies close to 100% without accumulating hydrogen. Moreover, the one-reactor hybrid platform is successfully used for efficient acetate production from electricity and CO2 by microbes previously reported to be inactive in bioelectrochemical systems.
Mesenchymal stem cells and their mitochondrial transfer: a double-edged sword
Authors: Li Cheng , Cheung Marco K.H. , Han Shuo , Zhang Zhao , Chen Ling , Chen Junhui , Zeng Hui , Qiu Jianxiang 
Published: April 2019

Abstract
Mitochondrial dysfunction has been linked to many diseases including organ degeneration and cancer. Mesenchymal stem cells/stromal cells (MSCs) provide a valuable source for stem cell-based therapy and represent an emerging therapeutic approach for tissue regeneration. Increasing evidence suggests that MSCs can directly donate mitochondria to recover from cell injury and rescue mitochondrial damage-provoked tissue degeneration. Meanwhile, cancer cells and cancer stromal cells also cross-talk through mitochondrial exchange to regulate cancer metastasis. This review summarizes the research on MSCs and their mitochondrial transfer. It provides an overview of the biology, function, niches and signaling that play a role in tissue repair. It also highlights the pathologies of cancer growth and metastasis linked to mitochondrial exchange between cancer cells and surrounding stromal cells. It becomes evident that the function of MSC mitochondrial transfer is a double-edged sword. MSC mitochondrial transfer may be a pharmaceutical target for tissue repair and cancer therapy.
Fulminante Lungenarterienembolie bei heparininduzierter Thrombozytopenie Typ II
Authors: Brück Simone , Skrabal Christian , Liebold Andreas , Mols Georg , Träger Karl 
Published: April 2019

ZusammenfassungBei einer 46-jährigen Patientin zeigt sich neben einem neu aufgetretenen Leberhämatom zusätzlich noch eine Lungenarterienembolie, so dass eine Antikoagulation mit Heparin begonnen wird. Daraufhin kommt es zu einer heparininduzierten Thrombozytopenie Typ II mit fulminanter Lungenarterienembolie, die eine Therapie mit einer venoarteriellen extrakorporalen Membranoxygenierung (va-ECMO) notwendig macht.
Thrombektomie beim Schlaganfall: anästhesiologische Aspekte
Authors: Wolf Alexander , Mölders Patrick , Yilmaz Umut 
Published: April 2019

ZusammenfassungDer Schlaganfall ist ein fokal-neurologisches Defizit auf dem Boden einer akuten Durchblutungsstörung. Therapieoptionen sind rt-PA-Lyse und neuroradiologische Thrombektomie. Letztere ist in Lokalanästhesie, Analgosedierung oder auch in Vollnarkose möglich. Dabei sollten wichtige (patho)physiologische und pharmakologische Aspekte in die anästhesiologische Versorgung von Schlaganfallpatienten einfließen, um das neurologische Ergebnis zu optimieren.
Wenn Plagegeister krank machen
Authors: Schmid HP Eva C. 
Published: April 2019

SummaryArthropoden (Zecken und Stechmücken) können beim Blutsaugen sogenannte Flaviviren übertragen auf Säugetiere und Vögel übertragen. Diese Viren können zum Beispiel beim Menschen Dengue-Fieber, West-Nil-Fieber, FSME oder das Zika-Fieber auslösen.
Practical applications and learning curve for EUS-guided hepaticoenterostomy: results of a large single-center US retrospective analysis
Authors: James Theodore , Baron Todd 
Published: April 2019

Abstract
Background and study aims Endoscopic ultrasound-guided hepaticoenterostomy (EUS-HE) is an effective method of endoscopic biliary drainage in cases where endoscopic retrograde cholangiopancreatography has failed or is deemed impossible. Indications for EUS-HE have expanded, resulting in increased interest by endoscopists to learn the procedure; however, few data exist on breadth of application or experience needed to develop proficiency. We describe utilization of EUS-HE for biliary decompression at a large tertiary referral center along with procedural learning curve.
Patients and methods Retrospective evaluation of 60 consecutive patients who underwent attempted EUS-HE by one endoscopist from February 2016 through June 2018. Procedures were divided into chronological and summative experience quartiles. We compared procedural success rate, procedural utilization, and procedure duration over time.
Results Sixty patients underwent attempted EUS-HE during the study period: 35 with surgically altered anatomy, 23 with malignant biliary obstruction, 35 outpatients, 35 females; median age, 66 years. The procedure was technically successful in 53 patients. Success rates by summative experience quartile were 80 %, 80 %, 93.3 % and 100 % respectively. Beginning at patient number 40, the remaining cases had a success rate of 100 %. Utilization increased from eight cases in the first chronological quartile to 28 in the fourth. There was no significant reduction in procedure duration over time.
Conclusion For an experienced endoscopist, EUS-HE could be performed effectively and safely after the experience of 40 cases. Limitations of this study include a single endoscopist and heterogeneous patient population with variable anatomy that may affect procedural success. Future studies should include data from multiple centers and endoscopists.
Recurrence of adenoma after curative endoscopic submucosal dissection for a rectal intramucosal adenocarcinoma in adenoma
Authors: Nakano Yoshiko , Toyonaga Takashi , Nishino Eisei , Inoue Taro , Shinjo Isato , Han-no Hajime , Tanaka Shinwa , Morita Yoshinori , Umegaki Eiji , Kodama Yuzo 
Published: April 2019

Abstract
Background and study aims A 71-year-old female underwent endoscopic submucosal dissection (ESD) for a subcircumferential lateral-spreading rectal tumor. Pathological examination showed an intramucosal adenocarcinoma in villous adenoma (size: 155 × 140 mm), which had been curatively resected with negative margins. However, follow-up colonoscopy revealed a tumor at the ulcer scar site, which soon grew into a circumferential lesion. Nineteen months after the first ESD procedure, additional ESD was performed for the recurrent lesion, which was resected en bloc without any adverse events, although severe fibrosis was noted in the submucosa. Pathological examination revealed a villous adenoma similar to the primary lesion with negative margins, but tumor cell nests were also present in the submucosa, which implied that tumor cell implantation had occurred during the first ESD. The post-ESD ulcer bed was subjected to argon plasma coagulation to prevent tumor recurrence after confirmation of the pathological results. There have not been any signs of recurrence during 9 years of follow-up.
Successful outcomes of a new combined solution of hyaluronic acid, chondroitin sulfate and poloxamer 407 for submucosal injection: animal survival study
Authors: Córdova Henry , Cuatrecasas Miriam , García-Rodríguez Ana , Montenegro Andrea , Melo Jordana , Rodríguez-de Miguel Cristina , Martínez-Pallí Graciela , Garcés-Durán Rodrigo , Llach Josep , Fernández-Esparrach Gloria 
Published: April 2019

Abstract
Background and study aims Endoscopic resection requires use of submucosal injection. This study was conducted to assess efficacy and impact on early healing of hyaluronic acid combined with chondroitin sulfate and poloxamer 407 (Ziverel) when used as a solution for submucosal injection.
Materials and methods Prospective and comparative study of gastric endoscopic mucosal resection (EMR) with three groups of two Yorkshire pigs. Six submucosal cushions were created in each animal by injecting 2 mL of Ziverel (Group 1) or succinylated gelatin (SG) (Group 2), enabling 12 EMR in each group. Submucosal cushions were created with Ziverel in Group 3, without resection. Electrosurgery unit settings were the same in all cases. EMR defects and injection sites were marked with clips. The animals were sacrificed 7 days later. EMR specimen size and duration of procedure were recorded. EMR specimens and EMR scars and injection sites were evaluated by a blinded pathologist.
Results We successfully performed 24 EMR (15 en-bloc and 9 piecemeal, without differences between groups 1 and 2). Mean EMR specimen dimensions were significantly larger in Group 1 (median 19 mm, range 6 – 40 vs 16.6 mm, range 5‑25; P = 0.019), without changing the electrocautery unit settings. Blinded histopathologist assessment of EMR specimens showed less fibrosis in the submucosa and a trend to fewer cautery artifacts with Ziverel and did not identify any significant differences in early healing of resection sites.
Conclusion The combination of Ziverel enables EMR and does not negatively affect early healing.
Lost in the hall of mirrors
Authors: Hutton Christopher 
Published: April 2019

AbstractThe categoryAryanand the paradigm of ideas associated with it remains highly controversial in contemporary India, and the history, status, and impact of this concept are contested at many levels. This paper starts with the assumption that the genesis of this concept lies in Western linguistic theorizing, and analyzes in outline the reception and impact of Aryan Invasion Theory and the postulation of an Aryan-Dravidian divide. Radical Hindu nationalists reject all aspects of the colonial scholarship of India; other Indian scholars see Western scholarship as authoritative to the extent that it falls within the framework of secular modernity. The argument made here is that the entire Aryan paradigm rests on a faulty set of academic presumptions and that its impact has been more long lasting and destructive than even the application of race theory to the understanding of India. In this sense the paper accepts the criticisms made by radical Hindu nationalists of colonial linguistics, and this raises further complex issues about knowledge production and application, scholarly expertise and authority.
Networking engagement in professional practices
Authors: Sancho Guinda Carmen 
Published: April 2019

Abstract
In this chapter I revisit the concept of metadiscursive engagement, track its major courses of research up to date, and discuss the interpersonal and discursive assumptions it entails. In light of these assumptions, I draw connections among the different contributions to this volume, present a networked picture of engagement resulting from their different understandings, and finally pinpoint challenges and emerging issues. The global and open vision of engagement offered here not only intends to bring together the three major theoretical frameworks resorted to in this volume (i.e. the Appraisal System, the Hylandian Metadiscourse Model and Multimodality), but also to suggest relating them with the idea of ‘distance’ between sender and addressee, explored by both the Appraisal System itself and the pragmatic theories of Proximisation and Politeness.
Rebuilding trust in the banking sector
Authors: McLaren-Hankin Yvonne 
Published: April 2019

Abstract
The financial crisis and various international scandals are widely viewed as having eroded trust in the banking sector, leading to calls for banks to act urgently to rebuild trust among stakeholders. This chapter examines one type of stakeholder communication, namely press releases on the subject of customer complaints, and aims to identify mechanisms by which the UK bank Barclays engages with customers as a means of regaining their trust. It shows that, through reader reference and transitivity structures in which readers (and writer) are projected into particular roles, Barclays seeks to demonstrate ability and benevolence, two of the three dimensions of an organisation’s trustworthiness (Mayer et al. 1995), and that the emphasis shifts from one to the other over time.
Verbal and nonverbal engagement devices in business persuasive discourse
Authors: Díez Prados Mercedes 
Published: April 2019

Abstract
The present chapter analyzes verbal and nonverbal engagement devices in four business pitches delivered by entrepreneurs in the British TV program Dragon’s Den. Taken for granted their eminently persuasive character, the purpose is to analyze the expression of engagement in verbal and nonverbal signs aiming at convincing potential investors of the product’s worth. A tridimensional analysis is carried out: firstly, structural features are identified following Daly and Davy’s (2016a and b) proposal. Secondly, verbal engagement devices are examined according to Martin and White’s (2005) Appraisal System and Hyland’s (2005a and b) Engagement Model. Thirdly, persuasive nonverbal signs are identified applying Cestero Mancera’s (2016 and 2017) taxonomy. Results show that while pitches are grossly monoglossic in their verbal realizations, nonverbal persuasive signs seem to acknowledge and guide the audience’s perception.