bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and psychometric appraisal of the Pain Management Self‐Efficacy Questionnaire
Authors: Macindo John Rey B. , Soriano Christian Albert F. , Gonzales Harvey Ross M. , Simbulan Paul Julius T. , Torres Gian Carlo S. , Que Jocelyn C. 
Published: April 2018

AbstractAimThe aim of this study was to develop and psychometrically evaluate the Pain Management Self‐Efficacy Questionnaire.BackgroundPain management competence and confidence are important in rendering safe and effective patient care. However, there is a lack of psychometrically sound instruments measuring pain management self‐efficacy.DesignTriphasic, prospective psychometric study.MethodA 55‐item Pain Management Self‐Efficacy Questionnaire was initially developed after extensive theoretical and literature review. The questionnaire was evaluated by content experts for content validity and a consensus was achieved after two iterations. After pretesting, the Pain Management Self‐Efficacy Questionnaire was distributed to 420 randomly selected pre‐graduate student nurses and registered nurses from a nursing institution and a tertiary hospital. Data collection was conducted from January 2015 ‐ 2016. Assessment parameters included basic item analysis, reliability analysis, floor and ceiling effects and construct validity using factor analysis and “known groups” technique. Replication analyses scrutinized two random halves of the sample.ResultsThe initial 55‐item questionnaire was reduced to 42 items after two iterations of validation. After preliminary factor analyses, the Pain Management Self‐Efficacy Questionnaire was reduced to 21 items. Final factor analysis produced a three‐factor model: Comprehensive, Evaluative and Supplemental Pain Management Self‐Efficacy. Construct validation using Kruskal–Wallis and Mann–Whitney tests showed group differences according to years of clinical experience and receipt of pain management training.ConclusionThe 21‐item Pain Management Self‐Efficacy Questionnaire demonstrated satisfactory psychometric properties and can be used to measure pain management self‐efficacy among nurses. Nevertheless, further psychometric validation is warranted accounting differences in culture and clinical practices.
Humanitarian nursing with Médecins Sans Frontières: Foregrounding the listening guide as a method for analysing oral history data
Authors: Golding Berenice , Hargreaves Janet 
Published: April 2018

AbstractAimsTo demonstrate how the listening guide contributed to oral history data analysis. To better understand the continuing inclination of nurses to engage in humanitarian work, foregrounding the nurses’ lives.BackgroundThe voice‐centred relational method or listening guide is a method of qualitative data analysis used to analyse oral history data.DesignA conventional approach to oral history interviews was adopted; intervention into the “flow” of participants’ narrative was kept to a minimum. A small number of prompts, how they came into nursing, recruitment to, life with and since Médecins Sans Frontières, were used.MethodsOral history interviews were conducted with seven nurses who had worked for Médecins Sans Frontières. Interviews were digitally recorded. This paper will demonstrate the application of the listening guide to historical data analysis and critique its applicability and value.The listening guide advocates four readings (listenings) of the text. Firstly, locating the plot in the narrative; secondly, actively listening for the use of “I?” (“we”, or “you”), the “self” in context of the story being told and “I poem” development; thirdly, listening for relationships and finally, locating accounts in relation to wider social, political and societal contexts.ResultsAnalysis revealed: “becoming”, “being” and “leaving” Médecins Sans Frontières as chronological thematic areas. At one extreme creating “I poems” foregrounded individual voices while cross‐referencing to contemporaneous records of world events locates this in an International context.ConclusionIt is argued that subjecting historical data to the listening guide can enable legitimate, creative exploration and analysis of data.
A self‐determination theory approach to health and well‐being in the workplace: Results from the sixth European working conditions survey in Spain
Authors: Gomez‐Baya Diego , Lucia‐Casademunt Ana M. 
Published: April 2018

AbstractResearch to date has underlined the importance of positive organizational psychology to improve health and psychological well‐being (PWB) in the workplace. The purpose of the present research is to test a model linking satisfaction of the basic psychological needs for autonomy, competence, and relatedness, as identified by self‐determination theory (SDT), and various individual work‐related outcomes, such as job satisfaction, PWB, and health problems in Spanish employees. Nationally representative data from the sixth European Survey on Working Conditions is Spain were used. Structural equation modeling was performed to examine the mediational role of job satisfaction in the relationships between psychological need satisfaction in the workplace and its consequences for PWB and health problems in Spanish employees. The results indicated that the fulfillment of basic psychological needs in the workplace is related to higher job satisfaction and that higher job satisfaction is associated with higher PWB and fewer health problems. Higher perceived competence and relatedness in the workplace were also related to greater PWB and fewer health problems. These results suggest that SDT is a valid approach that may guide interventions in the workplace to promote job satisfaction, PWB, and physical health in employees.
Laser‐assisted photodynamic therapy for superficial basal cell carcinoma and Bowen's disease: a randomized intrapatient comparison between a continuous and a fractional ablative CO2 laser mode
Authors: Genouw E. , Verheire B. , Ongenae K. , De Schepper S. , Creytens D. , Verhaeghe E. , Boone B. 
Published: April 2018

AbstractBackgroundLaser‐assisted photodynamic therapy is being explored as a method to enhance efficacy of photodynamic therapy (PDT).ObjectiveTo compare a continuous (CL) and a fractional (FL) ablative CO2 laser‐assisted methyl aminolevulinate (MAL) PDT in the management of superficial basal cell carcinoma (sBCC) and Bowen's disease (BD).MethodsThirty treatment areas in fifteen patients with inoperable, histologically verified sBCC or BD received CL or FL after intrapatient randomization. Laser treatment was followed by MAL application and illumination occurred 3 h later. This treatment was repeated after 2 weeks. An equivalence analysis was performed on the primary endpoint efficacy, while secondary endpoints pain, side‐effects and aesthetics were evaluated using paired samples tests. Patients were also asked for their preferred treatment.ResultsAn excellent efficacy of 92.9% (sBCC, 100%; BD, 80%) was found in both CL + PDT and FL + PDT after 12 months. Equivalence could not be established. Little pain was perceived in most patients during PDT illumination. PDT treatment in FL + PDT was less painful, significantly during the second treatment (P = 0.026). Side‐effects were mild to moderate with erythema being the most frequent immediate side‐effect, followed by oedema, crusting and burning sensation. Pigmentary changes occurred in 21% (CL + PDT) to 29% (FL + PDT), and aesthetics were good to excellent in all patients. CL + PDT and FL + PDT did not significantly differ in side‐effects (P = 0.219–1.000) or aesthetics (P = 0.157–1.000).ConclusionsResults in this pilot study support the promising role of laser‐assisted PDT. Both treatment arms demonstrated the same efficacy as well as comparable side‐effects and aesthetics. PDT illumination was significantly less painful in the FL + PDT group, suggesting a preference for FL + PDT. The authors recommend further investigation with a larger sample size, a subgroup analysis between sBCC and BD and comparison of different treatment protocols before one technique could be preferred to another.
Laser hair removal after surgery vs. surgery alone for the treatment of pilonidal cysts: a retrospective case–control study
Authors: Kelati A. , Lagrange S. , Le Duff F. , Lacour J.‐P. , Benasaid R. , Breaud J. , Passeron T. 
Published: April 2018

AbstractBackgroundBased on the presumed role of hair in pilonidal cyst (PNC) pathogenesis, laser epilation has been used to decrease recurrences. However, most of the data rely on case reports and uncontrolled series, and the rare controlled studies reported conflicting results. The objective of this study was to investigate the efficacy of laser hair removal (LHR) to decrease the recurrence rate after surgery of PNC vs. surgery alone.MethodsWe conducted a retrospective monocentric case–control study in the departments of Pediatric Surgery and Laser Center of Dermatology of the University Hospital of Nice in France from January 2010 to June 2017. We included all patients with PNC who had surgery during the period, and we compared patients who had LHR after surgery to those having surgery alone. The main outcome was the prevalence and number of recurrences of PNC in each group.ResultsTwelve patients – eight treated with alexandrite laser and four patients with Nd : YAG – were included in the cases group; 29 patients treated with surgery alone were included in the control group. The mean and the median number of laser sessions were 4.2 and 5, respectively (extremes 1–10). The postsurgical recurrence rate without LHR was 51.7%, and their number varied from 1 to 3. A significant decrease in the recurrence rate was observed after LHR (8.3%) compared to control group (P < 0.001). The median duration before recurrence was 14 months for surgery alone. The median follow‐up was 18 months (1–30 months) for LHR group and 30 months (6–72 months) for surgery alone group. Two patients had abnormal healing or persistent sinus after surgery alone compared to none in the group who undergone laser procedure after surgery.ConclusionsLaser hair removal decreases the risk of delayed healing and of recurrences of PNL after surgical procedure.
High volume of polysorbate‐containing (Tween® 80) solutions induces false‐positive results in intradermal test
Authors: Wagner N. , Podda M. 
Published: April 2018

AbstractBackgroundIntradermal test is used to detect causative allergens in IgE‐mediated hypersensitivity. The surfactant polysorbate 80 can be added to intradermal test solutions to more reliably dissolve the allergen and ensure a constant bioavailability of the injected allergen. Polysorbate 80 has, however, some histamine‐releasing properties which could blur the difference to the histamine‐induced wheal. Routinely serving as a control.ObjectiveAllergen‐free polysorbate 80 containing (0.005%) test solutions were therefore systematically tested at different injection volumes to see whether polysorbate can falsify skin reactions and if yes whether conditions can be identified to avoid this.MethodsIn a partly blinded study, 30 patients were tested intracutaneously at the back: each received at three separate sites 0.05 mL of polysorbate‐containing solvent, 0.02 mL polysorbate‐containing solvent as well as 0.02 mL polysorbate‐free solvent. After 15 min, wheal and erythema were documented and planimetrically quantified.ResultsUnexpectedly 23 of 30 (77%) patients showed false‐positive test reactions to the volume of 0.05 mL of the polysorbate‐containing solvent whereas the polysorbate‐containing solvent with 0.02 mL and the polysorbate‐free solvent with 0.02 mL injection volume had no reinforcing effect on skin test reaction.ConclusionsDifferent volumes of polysorbate‐containing solutions, all recommended by manufacturers for intradermal tests, may significantly influence test results. The study shows that a polysorbate 80 used at 0.005% in solvents for intradermal test to provide a better bioavailability of allergens produces false‐positive reactivity in a surprising and a hitherto unknown 77% if the 0.05 mL volume is injected. It is, therefore, mandatory to strictly adhere to an intradermal test volume of 0.02 mL as only then falsifying effects of the polysorbate additive are avoided, and comparable test results are ensured.
Variation in expression and activity pattern ofmmp2andmmp9on different time scales in the regenerating caudal fin ofPoecilia latipinna
Authors: Murawala H. , Patel S. , Ranadive I. , Desai I. , Balakrishnan S. 
Published: April 2018

Alteration in the expression pattern of matrix metalloproteinase (MMP)2 and MMP9 was studied in the regenerating caudal fin ofPoecilia latipinnaimmediately following amputation until the new tissues gained structural integrity. Timed expression pattern of these two MMPs was studied at enzyme, transcript as well as protein levels. Additionally, both the gelatinases were localized in the regenerating caudal fin during three specific stages of regeneration. The results revealed a progressive increase in the expression of MMP2 starting at 1 h post amputation (hpa), indicating its possible role in the remodelling of extracellular matrix early on during caudal‐fin regeneration. Nevertheless, a reduction in transcript level expression of MMP2 at 6 hpa and 12 hpa stages, points towards a possible transcriptional regulation, to further moderate its activity. As observed in the case of MMP2, expression of MMP9 too increased from 1 hpa and remained elevated until 5 dpa. However, the active MMP9 revealed its presence only 12 hpa onwards. Moreover, both the gelatinases were localised in the apical epithelial cap and in the progress zone at wound epithelium (1 dpa) and blastema (60 hpa) stages respectively. Further, during early differentiation stage (5 dpa), high intensities of MMP2 and MMP9 were localized in the newly formed actinotrichia as compared with the tissue proximal to it. Based on the results, it could be construed that the controlled up‐regulation of MMP2 and MMP9 from 1 hpa until the early differentiation stage ensures a regulated digestion of extracellular matrix, perhaps to facilitate the recruitment, proliferation, morphogenesis and re‐patterning of resident stem cells during caudal fin regeneration inP. latipinna.
Research protocol: The initiation, design and establishment of the Global Angelman Syndrome Registry
Authors: Tones M. , Cross M. , Simons C. , Napier K. R. , Hunter A. , Bellgard M. I. , Heussler H. 
Published: April 2018

AbstractBackgroundAngelman syndrome (AS) is a rare neurodevelopmental disorder affecting between 1 in 15 000 and 1 in 24 000 individuals. The condition results in severe developmental and expressive language delays, motor impairments and a unique behavioural phenotype consisting of excessive laughter, smiling and sociability. While many studies have contributed knowledge about the causes and natural history of the syndrome, large scale longitudinal studies are required to advance research and therapeutics for this rare syndrome.MethodThis article describes the protocol for the Global Angelman Syndrome Registry, and some initial findings. Due to the rarity of AS and the variability in symptom presentation, the registry team will strive for complete case ascertainment. Parents and caregivers will submit data to the registry via a secure internet connection. The registry consists of 10 modules that cover patient demographics; developmental, diagnostic, medical and surgical history, behaviour and development, epilepsy, medications and interventions and sleep. Results: Since its launch at https://angelmanregistry.info in September 2016, almost 470 individuals with AS have been signed up to the registry worldwide: 59% are from North and South America, 23% are from Europe, 17% are from the Asia Pacific region and 1% are from the Middle East or Africa. The majority of registrants are children, with only 16% aged over 20 years. Most participants indicated a chromosome deletion (76%), with fewer participants indicating a mutation, uniparental disomy or imprinting defect (20%).ConclusionFindings indicate a need to consider recruitment strategies that target caregivers of older children and adults, and parents and caregivers from non‐English speaking backgrounds.
A momentary lapse of reason? Gezi in social‐historical perspective
Authors: Sofos Spyros A. 
Published: April 2018

AbstractThrough the social‐historical contextualization of Gezi protests and drawing on the works of Badiou and Turner this article conceptualises the protests as a ‘happening’ or ‘event’ characterized by rupture and liminality. Without underestimating their importance as a meaning creation process, it is argued that the visions inspiring Gezi have been/are in sharp contrast to the version of democracy shaped by the fears and aspirations of at least a plurality of the country's citizenry and enacted by the Justice and Development Party (AKP). At the same time, these visions remain largely incomprehensible to the Kemalist and nationalist opposition. The paper therefore suggests that Gezi should be located outside the linear time and conventional topography of Turkish politics and interpreted as a brief, powerful moment of rupture in a political system where both the incumbent political forces and the opposition and their constituencies are resisting change and consider extra‐institutional ‘antipolitics’ as a threat.
Antimutagenic constituents from Monanthotaxis caffra (Sond.) Verdc.
Authors: Makhuvele Rhulani , Foubert Kenn , Apers Sandra , Pieters Luc , Verschaeve Luc , Elgorashi Esameldin 
Published: April 2018

Abstract

Objectives
Monanthotaxis caffra (Sond.) Verdc. (Annonaceae) has been reported to possess antitumoural properties. Preliminary screening showed that the crude methanolic leaf extract had strong antimutagenic effects against aflatoxin B1-induced mutagenicity. The aim of this study was to isolate and evaluate the antimutagenic properties of the active constituents from M. caffra.

Methods
Different chromatographic, spectroscopic and spectrometric techniques were used for the isolation and identification of the antimutagenic constituents. The antimutagenic effect of the extract and compounds was evaluated using Ames, Vitotox and Comet assays.

Key findings
Bioassay-guided fractionation of the methanolic leaf extract yielded two antimutagenic compounds identified as (+)-crotepoxide and 5,6-diacetoxy1-benzoyloxymethyl-1,3-cyclohexadiene. Crotepoxide had strong antimutagenicity in the Vitotox assay with an IC50 value of 131 μg/ml. 5,6-Diacetoxy-1-benzoyloxymethyl-1,3-cyclohexadiene showed strong antimutagenic activity in the Ames assay with an IC50 value of 348.9 μg/plate and no antimutagenic activity in the Vitotox test. Furthermore, the compound was able to inhibit, block or prevent biotransformation of aflatoxin B1 by repressing the proteins involved in transcription.

Conclusions
Crotepoxide and 5,6-diacetoxy-1-benzoyloxymethyl-1,3-cyclohexadiene have the potential to mitigate the risks arising from consumption of aflatoxin B1-contaminated food and feed.
Brain natriuretic peptide serum levels in periodontitis
Authors: Leira Y. , Blanco J. 
Published: April 2018

Background and ObjectiveN‐terminal‐pro‐brain natriuretic peptide (NT‐proBNP) is expressed during inflammation and considered to be a biomarker of cardiovascular disease (CVD). In the last decade, evidence has suggested that periodontitis is associated with CVD. However, little is known of the potential role of this peptide in periodontal disease. The aims of this study were to examine whether the levels of NT‐proBNP in serum are increased in periodontal disease and if there is a relationship to severity of periodontitis.Material and MethodsA case‐control study was carried out, in which serum samples were collected from 40 patients with periodontitis and from 40 nonperiodontitis individuals. Full periodontal examination was performed in all subjects. Demographic information, and clinical and history of certain diseases were also recorded. Determinations of serum NT‐proBNP and high‐sensitivity C‐reactive protein concentrations were carried out in an independent laboratory.ResultsThe levels of NT‐proBNP in serum were significantly higher in patients with periodontitis compared with controls (87.9 pg/mL vs 29.9 pg/mL, P < .0001). In addition, as periodontal disease progressed, the levels of NT‐proBNP increased. Multivariable regression analysis showed that the periodontal inflamed surface area, a measure of periodontal inflammation and disease activity, was the only periodontal parameter significantly associated with elevated concentrations of NT‐proBNP in serum (R2 = .777, P < .0001).ConclusionIn periodontitis, increased serum NT‐proBNP levels are observed in comparison with individuals without periodontitis. Moreover, the greater the degree of periodontal destruction, the higher the levels of NT‐proBNP in serum.
Searching for the genetic footprint of ancient and recent hybridization
Authors: Vallejo‐Marín Mario 
Published: April 2018

Determining the long‐term consequences of hybridization remains a central quest for evolutionary biologists. A particular challenge is to establish whether and to what extent widespread hybridization results in gene flow (introgression) between parental taxa. In this issue of Molecular Ecology, Jordan et al. () search for evidence of gene flow between two closely related species of Geum (Rosaceae), which hybridize readily in contemporary populations and where hybrid swarms have been recorded for at least 200 years (Ruhsam, Hollingsworth, & Ennos, ). The authors find mixed evidence of ancient introgression when analysing allopatric populations. Intriguingly, when analysing populations of a region where the two species occur either mixed in the same population or in close proximity, and where hybrids are presently common, Jordan and colleagues find that the majority of randomly sampled individuals analysed (92/96) show no evidence of introgression (defined as individuals with admixture coefficients of <1%). The few individuals identified as hybrids are shown to likely be F1 or early‐generation backcrosses, indicating that even in sympatric regions, hybridization does not penetrate beyond a few generations. Based on their findings, Geum seems to be an example of little to no introgression despite contemporary hybridization.
Variant‐specific and reciprocal Hsp40 functions in Hsp104‐mediated prion elimination
Authors: Astor Michael T. , Kamiya Erina , Sporn Zachary A. , Berger Scott E. , Hines Justin K. 
Published: April 2018

SummaryThe amyloid‐based prions of Saccharomyces cerevisiae are heritable aggregates of misfolded proteins, passed to daughter cells following fragmentation by molecular chaperones including the J‐protein Sis1, Hsp70 and Hsp104. Overexpression of Hsp104 efficiently cures cell populations of the prion [PSI+] by an alternative Sis1‐dependent mechanism that is currently the subject of significant debate. Here, we broadly investigate the role of J‐proteins in this process by determining the impact of amyloid polymorphisms (prion variants) on the ability of well‐studied Sis1 constructs to compensate for Sis1 and ask whether any other S. cerevisiae cytosolic J‐proteins are also required for this process. Our comprehensive screen, examining all 13 members of the yeast cytosolic/nuclear J‐protein complement, uncovered significant variant‐dependent genetic evidence for a role of Apj1 (antiprion DnaJ) in this process. For strong, but not weak [PSI+] variants, depletion of Apj1 inhibits Hsp104‐mediated curing. Overexpression of either Apj1 or Sis1 enhances curing, while overexpression of Ydj1 completely blocks it. We also demonstrated that Sis1 was the only J‐protein necessary for the propagation of at least two weak [PSI+] variants and no J‐protein alteration, or even combination of alterations, affected the curing of weak [PSI+] variants, suggesting the possibility of biochemically distinct, variant‐specific Hsp104‐mediated curing mechanisms.