bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of cognitive training on cognitive function in patients after cardiac surgery: A systematic review and meta-analysis of randomized controlled trials
Authors: Zhang Rongxiang , Zhu Chenyang , Chen Shiqi , Tian Feng , Huang Pingping , Chen Yuan 
Published: November 2024

Background:
Postoperative cognitive deficits frequently occur in patients undergoing cardiac surgery, leaving them with reduced cognitive function. Cognitive training has been shown to improve cognitive function, however, the role in patients after cardiac surgery is unclear. In this study, we aimed to evaluate the effectiveness and safety of cognitive training in patients undergoing cardiac surgery.

Method:
A systematic search of PubMed, Embase, Cochrane Library, CINAHL, Ovid Medline, Web of Science, CNKI, and Wanfang was conducted until March 2024. The risk of bias was assessed using the Cochrane Risk of Bias Tool. Data were meta-analyzed using RevMan 5.4 software. Potential bias and reliability of evidence were fairly assessed by using the Cochrane risk of bias method and the GRADE evidence grading method.

Results:
A total of 16 studies involving 1335 cardiac surgery patients were included in this study. Compared with the control group, the cognitive training group had a significantly lower incidence of postoperative cognitive dysfunction (RR 0.35, 95% CI 0.18–0.65, P = .001), significantly improved cognitive function (MD 2.54, 95% CI 1.27–3.81, P < .001), and a significantly higher quality of life-mental component (MD 5.22, 95% CI 2.32–8.13, P < .001), anxiety (MD −6.05, 95% CI −10.96 to −1.15, P = .02) and depression (MD −3.97, 95% CI −7.15 to −0.80, P = .01) were significantly improved between groups. However, the differences were not statistically significant for postoperative delirium (RR 1, 95% CI 0.38–2.65, P = 1.00) and postoperative hospitalization (MD −0.95, 95% CI −2.90 to 1.00, P = .34).

Conclusions:
The present study, based on a low to moderate quality of evidence, suggests that cognitive training improves cognitive functioning, reduces the incidence of postoperative cognitive dysfunction, and has a positive impact on anxiety and depression in patients undergoing cardiac surgery. However, current evidence does not allow for the determination of effects on quality of life, postoperative delirium, and postoperative length of stay.
Prevalence of HIV/AIDS among pregnant women in North American region: A systematic review and meta-analysis
Authors: Shabbir Nosheena Akhter , Kant Sara Bashir , Rashid Kainat , Hafeez Uzma , Akbar Aiza Ali , Batool Syeda Wajiha , Pranto Alif Hasan , Zaman Jemema , Shahriyer Tonmoy Hasan , Islam Md Rashedul , Meem M. M. Rubaiyat Muntasir , Islam Dewan Zubaer , Suez Ehsan , Khandker Shahad Saif , Akbar Amna , Khattak Muhammad Iftikhar , Ali Amir Iqbal , Jadoon Sarosh Khan , Shakeel Attarab , Zubair Maryam , Alvi Sarosh 
Published: November 2024

Background:
As a major maternal health concern, the prevalence of human immunodeficiency virus (HIV) among pregnant women was previously investigated in European, African, and Latin American regions other than the North American region. This study analyzed the prevalence of HIV among pregnant women in the North American region including 3 major countries: the USA, Canada, and Mexico.

Methods:
Relevant studies were screened from 3 online databases: Google Scholar, PubMed, and ScienceDirect using specific search keywords. Ultimately, 10 studies of the North American region were included with a total of 339,831 pregnant women residing in the USA, Canada, and Mexico.

Results:
The overall pooled prevalence was 0.6% (95% confidence interval [CI]: 0.4–0.8) with a high degree of heterogeneity (I
2 = 97%). Pooled prevalence rates of HIV among pregnant women in Canada, Mexico, and the USA were 0.3% (95% CI: 0.1–0.5), 0.5% (95% CI: 0.2–0.8), and 2.3% (95% CI: 0.0–5.7), respectively with high degrees of heterogeneity.

Conclusion:
The overall prevalence rate of HIV among pregnant women in the USA, Canada, and Mexico was minimal as compared with the countries of Eastern Europe, sub-Saharan Africa, or Latin America. Awareness, adequate testing and healthcare facilities, better socioeconomic, and geopolitical conditions might be crucial to lowering the prevalence of HIV among pregnant women.
Global research trends of virtual simulation technology in public health education and training: A visual analysis based on CiteSpace and VOSviewer
Authors: Dong Yonghai , Ding Sheng , Mao Xiangqun , Liu Xiaoqing 
Published: November 2024

Background:
In response to global health challenges, implementing innovative educational strategies is crucial for preparing public health professionals with the required skills. This study employed CiteSpace and VOSviewer to visually analyze 3 decades of research on virtual simulation technology in public health education and training. The visual knowledge map created aimed to uncover the research trends, key areas of interest, and emerging frontiers in this domain.

Methods:
Leveraging the Web of Science core collection database and employing bibliometric methods, the CiteSpace and VOSviewer softwares were used to analyze the annual publication, literature distribution, country/institution distribution, author distribution, co-cited literature, and keywords related to the virtual simulation technology in public health education and training over the past 30 years. A visual map was then created.

Results:
A total of 1241 research articles were involved in the analysis. The yearly publication count and citations had an overall upward trend, with a significant increase in the number of publications since 2019. The studies in this domain are mainly concentrated in developed countries, particularly in Europe and the United States. The United States emerged as the frontrunner in terms of the number of publications, while both the United Kingdom and the United States exhibited the highest mediation centrality and exerted the greatest international influence. Harvard University made the most substantial contribution with 50 articles and exhibited an institutional cooperation centrality of 0.12. The paper entitled “Using thematic analysis in psychology” had the highest number of citations (28). The primary research hotspots included disaster medicine, telehealth, and virtual reality, while aspects such as digital health, augmented reality, and serious games had consistent continuity. An emerging research frontier, Post-2020, included several topics such as medicine, COVID-19, quality, virtual learning, rehabilitation, depression, and strategy.

Conclusion:
This study presents the inaugural comprehensive analysis of global trends, hotspots, frontiers, and advancements in the implementation of virtual simulation technology in public health education and training, utilizing CiteSpace and VOSviewer software. The research findings reveal a significant surge in publications since 2019, with a particular emphasis on disaster medicine, telehealth, and virtual reality, indicating the versatility and promise of virtual simulation in the changing educational environments. These findings emphasize the significance of virtual simulation as a dynamic and progressive tool in public health education, proposing a promising direction for future research and practical applications.
Improved injection site reactions after switching from adalimumab reference to adalimumab biosimilar LBAL for ulcerative colitis: A case report
Authors: Hirakawa Yudai , Kakiuchi Toshihiko , Yoshiura Masato , Kikkawa Nao 
Published: November 2024

Rationale:
Adalimumab (ADA) is an antitumor necrosis factor agent that is used for the treatment of inflammatory bowel disease. However, its cost has resulted in varying degrees of restricted access across global healthcare economies. Biosimilars are agents that contain a similar version of the active substance of an already approved original biologic agent and are intended to be used for the same indication as the reference product. In general, biosimilars follow the originator; therefore, information on its efficacy and safety had been few. Some studies have reported on replacement of the originator with a biosimilar of the same efficacy because of medical reasons.

Patient concerns:
A 15-year-old girl with steroid-dependent ulcerative colitis that relapsed after vedolizumab was treated with ADA reference. Six weeks after starting ADA reference, her gastrointestinal symptoms had completely resolved, however, immediately after the eighth dose of ADA reference, redness, swelling, and pruritus were noted at the injection site on the left thigh.

Diagnosis:
Allergic reaction caused by the ADA reference.

Intervention:
ADA reference was changed to ADA biosimilar LBAL.

Outcomes:
ADA biosimilar LBAL was continued without any symptoms, such as local swelling, redness, or itching. In addition, there was no deterioration of gastrointestinal symptoms.

Lessons:
We showed the efficacy and safety of ADA biosimilar LBAL as an alternative to ADA reference, which caused injection site reactions. Changing from ADA reference to ADA biosimilar because of adverse events may be an option that needs careful observation, considering that the originator and the biosimilar are not exactly the same.
Catheter ablation in a monochorionic diamniotic twin pregnancy: A case report and literature review
Authors: Jia Yanxi , Liao Hua , Hu Qing , Liu Hongyan , Zeng Zhaomin , Yu Haiyan 
Published: November 2024

Rationale:
Preexcitation syndrome is an uncommon congenital cardiac disorder that impairs the normal cardiac conduction system. Radiofrequency ablation is one of the most effective treatments for this condition. Nevertheless, radiofrequency ablation is rare in women with preexcitation syndrome during pregnancy.

Patient concerns:
A 33-year-old woman with monochorionic diamniotic twin pregnancy complicated by sinus arrhythmia with ventricular preexcitation at 14 weeks and 5 days of gestation, with paroxysmal palpitations and shortness of breath at 16 weeks with paroxysmal supraventricular tachycardia with preexcitation syndrome and a heart rate ranging from 180 to 225 beats per minute.

Diagnoses:
The pregnant occurred sudden palpitations and shortness of breath in the shower. Electrocardiography revealed paroxysmal supraventricular tachycardia, and electrophysiological study revealed preexcitation syndrome (dominant accessory route of the left free wall) with atrioventricular reentrant tachycardia.

Interventions:
Radiofrequency catheter ablation was performed at 20 weeks.

Outcomes:
Symptoms of preexcitation syndrome resolved after the radiofrequency catheter ablation, and 2 healthy infants were delivered at 36 weeks and 2 days of gestation by cesarean section.

Lessons:
Preexcitation syndrome may result in life-threatening arrhythmias such as supraventricular tachycardia during pregnancy and delivery. It might be efficiently controlled through optimal treatment by a multidisciplinary team, which would effectively minimize arrhythmia risk events during pregnancy and improve maternal–fetal outcomes. Based on the patient’s individual situation, radiofrequency ablation may be a procedure in pregnant women with preexcitation syndrome.
Microvenular hemangioma located in the breast: a case report
Authors: Dong Mengting , Zhang Likun , Sheng Jiayu , Chen Junyi , Jiang Ke 
Published: November 2024

Abstract
Microvenular hemangioma (MVH) is a rare, benign type of hemangioma that typically presents as a solitary, slow-growing, red-to-violaceous asymptomatic papule, plaque, or nodule. It most commonly locations of the lesions on the limbs and trunk, with no prior reports of MVH confined to the breast. We present the first reported case of multiple MVH confined to the breast in a 17-year-old female patient. The case is notable for its unique and atypical clinical features. Diagnosing MVH is challenging and requires routine pathological and immunohistochemical staining. Histological testing revealed typical features of MVH, irregularly branched small or medium vascular interstitial spaces lined with benign endothelial cells and positivity for CD34 and CD31. This case emphasizes the need to consider MVH in the differential diagnosis of breast vascular lesions, particularly in young patients. It also underscores the importance of histological and immunohistochemical analyses for an accurate diagnosis.
Decedents Found in Bathtubs
Authors: Faust Bethany , Dye Amanda , Gill James R. 
Published: November 2024

Abstract
We investigated deaths in bathtubs for criteria to assist in death certification. We reviewed 252 deaths in which the person was found dead in a bathtub/shower at home. We extracted cause, manner, age, clad or unclad, water in the bathtub, water running, and face submersion. Of the 252 deaths, heart disease was the leading cause of death (129), followed by intoxication (47), trauma (22), drowning (28), and other diseases (26). The manners of death were 155 natural, 64 accident, 30 suicide, and 3 homicides. All drowning fatalities involved the decedent having their face submerged. Intoxication and natural deaths had submersion of the face in 15% and 12% of deaths, respectively. For nondrowning causes of death, the majority were without face submersion. For natural deaths, the predominant circumstance was the individual discovered unclad with water in the bathtub and the face not submerged. Conversely, for suicides, the majority were found clad without water. A comprehensive investigation incorporating a scene examination, gathering of the medical and psychosocial history, and typically an autopsy is needed to certify the cause and manner of death. Face submersion alone is not diagnostic of a drowning and must be interpreted with the medical history and autopsy findings.
A Rare Case of Neonatal Death Due to a Cranial Vault Fracture Following the Use of Obstetric Vacuum Extractor and Review of Literature
Authors: Caputo Fiorella , Caristo Isabella , Barranco Rosario , Vallega Bernucci Luca , Torielli Flaminia , Ventura Francesco 
Published: November 2024

Abstract
In the course of childbirth, if complications arise during the expulsion of the fetus, instruments can be employed to facilitate delivery. The vacuum extractor, or ventouse, is considered a relatively safe tool associated with a low rate of complications and mortality. However, it is crucial that the instrument be applied in the correct position and that the pulling force is not excessive to minimize damage to the fetal cranial structures. Fractures of the cranial vault following the application of obstetric vacuum extraction are very rare events.
This paper presents a case of perinatal death due to cranial vault fracture and subgaleal hematoma following vacuum extractor application. Only through postmortem examination was it possible to identify incorrect positioning of the ventouse, which was lateralized compared to the recommended position. The misplacement of the device led to an imbalance of forces, causing the cranial vault fracture that led to the infant's demise.
A thorough postmortem examination is essential in cases of perinatal death resulting from operative delivery to ascertain the cause of death and reconstruct the dynamics of events during childbirth. In particular, the examination of injuries on the decedent neonate may reveal improper positioning of the instrument.
Condylar Volumetric Asymmetry Predicts Need for Surgical Intervention in Patients With Cleft Lip and/or Palate
Authors: Romeo Dominic J. , Oral Kaan T. , Ryan Isabel A. , Lenz Theodor , Massenburg Benjamin B. , Ng Jinggang J. , Wu Meagan , Du Steven , Bartlett Scott P. , Swanson Jordan W. , Taylor Jesse A. 
Published: November 2024

Objective:
In this study, the authors investigate the associations between the mandibular condyle and facial asymmetry in patients with cleft lip and/or palate (CLP).

Methods:
Condylar volume was calculated by measuring the bony volume of the posterior mandible superior to the sigmoid notch in skeletally mature patients with CLP and controls. Relationships between condylar asymmetry, facial deviation, and malocclusion were compared using t-tests, correlation analysis, and receiver operating characteristic curves.

Results:
This study included 118 patients: 60 with CLP preparing for orthognathic surgery, 17 with CLP who did not undergo orthognathic surgery, and 41 controls. Condylar volumes in patients with CLP preparing for orthognathic surgery were more asymmetric than those with CLP not requiring surgery (16.4 ± 17.4% vs 7.1 ± 6.0%, P = 0.03). Patients with CLP who did not undergo orthognathic surgery and controls had similar degrees of condylar asymmetry (7.1 ± 6.0% vs 5.9 ± 3.8%, P = 0.35). Condylar asymmetry correlated with chin deviation (P < 0.01). Patients with CLP and clinically significant chin deviation (>4 mm) had more asymmetric condyles than those without significant chin deviation, and the chin usually deviated toward the smaller condyle (P = 0.03). Condylar asymmetry >11% predicted undergoing orthognathic surgery in CLP with 70% sensitivity and 67% specificity.

Conclusion:
Condylar asymmetry is associated with facial asymmetry in patients with CLP, and the facial midline often deviates towards the smaller condyle. Condylar volumetric asymmetry in patients with CLP may be a predictor for needing orthognathic surgery, useful information for surgeons and families alike.
Enhancing cryopreservation of human iPSCs: Bottom-up vs Conventional freezing geometry
Authors: Teodoro Fernando , El-Guendouz Soukaina , Neves Rafaela , Duarte Andreia , Rodrigues Miguel A. , Melo Eduardo P. 
Published: November 2024

AbstractInduced pluripotent stem cells (iPSCs) hold large potential on regenerative medicine due to their pluripotency and unlimited self-renewal capacity without the ethical issues of embryonic stem cells. To provide quality-controlled iPSCs for clinical therapies, it is essential to develop safe cryopreservation protocols for long-term storage, preferable amenable for scale-up and automation. We have compared the impact of two different freezing geometries (bottom-up and conventional radial freezing) on the viability and differentiation capability of human iPSCs. Our results demonstrate that the bottom-up freezing under optimized conditions significantly increases iPSCs viability, up to 9% for the cell membrane integrity and up to 21% for the cell metabolic state, compared to conventional freezing. The improvement achieved for bottom-up versus conventional freezing was maintained after scale-up from cryogenic vials to 30 mL bags, highlighting the method’s potential for clinical applications. These findings show that bottom-up freezing can offer a more controlled and scalable cryopreservation strategy for iPSCs, promoting their future use in regenerative medicine.
Multi-omics analysis reveals CMTR1 upregulation in cancer and roles in ribosomal protein gene expression and tumor growth
Authors: Campeanu Ion John , Jiang Yuanyuan , Afisllari Hilda , Dzinic Sijana , Polin Lisa , Yang Zeng-Quan 
Published: November 2024

AbstractThe mRNA cap methyltransferase CMTR1 plays a crucial role in RNA metabolism and gene expression regulation, yet its significance in cancer remains largely unexplored. Here, we present a comprehensive multi-omics analysis of CMTR1 across various human cancers, revealing its widespread upregulation and potential as a therapeutic target. Integrating transcriptomic and proteomic data from a large set of cancer samples, we demonstrate that CMTR1 is upregulated at the mRNA, protein, and phosphoprotein levels across multiple cancer types. Functional studies using CRISPR-mediated knockout and siRNA knockdown in breast cancer models show that CMTR1 depletion significantly inhibits tumor growth bothin vitroandin vivo. Transcriptomic analysis reveals that CMTR1 primarily regulates ribosomal protein genes and other transcripts containing 5’ Terminal Oligopyrimidine (TOP) motifs. Additionally, CMTR1 affects the expression of snoRNA host genes and snoRNAs, suggesting a broader role in RNA metabolism. Mechanistically, we propose that CMTR1’s target specificity is partly determined by mRNA structure, particularly the presence of 5’TOP motifs. Furthermore, we identify a novel CMTR1 inhibitor, N97911, throughin silicoscreening and biochemical assays, which demonstrates significant anti-tumor activityin vitro. Our findings establish CMTR1 as a key player in cancer biology, regulating critical aspects of RNA metabolism and ribosome biogenesis, and highlight its potential as a therapeutic target across multiple cancer types.
Low RT-based Genome Editing Fidelity in Mouse Hepatocytes: Challenges and Solutions
Authors: Dunyak Maike Thamsen , Hanna Patrick , Nan Angela X. , von Stetina Jessica , Pokharel Rejina , O’Hara Mollie , Estes Brett J.G. , Zheng Kangni , Svenson Sonke , Andresen Jason , Li Wei , Agrawal Mini , Hughes Chase , Spicer Makayla , Choudhary Vimal , Kelley Lily , Wang Jie , Meagher Martin , Xie Jenny , Mukherjee Swati , Finn Jonathan D. 
Published: November 2024

Abstract/SummaryIntegrase-mediated Programmable Genomic Integration (I-PGI) uses a Cas9 nickase (nCas9) with a reverse transcriptase (RT), to write a large serine integrase (LSI) target site (attB/P, here called “beacon”) in a programmed location. Co-delivery of the LSI and a DNA template containing the cognate recognition site results in precise integration of the template in a specific genomic location. While we were able to achieve high-fidelity beacon placement in a range of primate cycling and non-dividing cells, when translating our technology into anin vivorodent model (liver) we surprisingly observed very low beacon fidelity, with the vast majority of beacons being unsuitable for integration. This phenomenon was independent of mouse strain, but was specific to non-dividing cells, as a cycling mouse hepatocyte cell line (Hepa1-6) demonstrated very high levels of fidelity. To address this issue we utilized neonatal mice, which have a much higher proportion of proliferating hepatocytes than adult mice. This resulted in a significant increase in the placement of high-fidelity beacons, and achieved functional gene expression after I-PGI in a therapeutically relevant target site. In an alternate approach, we engineered transgenic mice with intact beacons placed in specific genomic locations, allowing us to optimize integrase and DNA template dosing and kinetics. In summary, we have identified a previously undescribed challenge when using RT-based editing to write long sequences (~40 bp) in non-dividing rodent hepatocytes. This phenomenon was specific to rodents and was not observed in primate dividing or non-dividing cells. This previously unidentified challenge using RTs will limit the use of I-PGI in mouse models, however here we describe two methods that address this issue.
Differential Roles of Longevity Assurance GenesLAG1andLAC1in Regulating Endoplasmic Reticulum stress and Lipid Homeostasis inSaccharomyces cerevisiae
Authors: Mathivanan Arul , Nachiappan Vasanthi 
Published: November 2024

ABSTRACTYeast ceramide synthesis is regulated by two homologous genes,LAC1andLAG1,each with distinct physiological roles, especially under ER stress conditions. This study examines their specific functions in lipid homeostasis and the ER stress response. DeletingLAG1enhances cell growth and survival under ER stress, whereasLAC1deletion does not provide similar resistance. In contrast,LAG1deletion significantly impacts phospholipid and neutral lipid metabolism without altering the expression of key ER stress response genes. In comparison,LAG1overexpression, unlikeLAC1overexpression, severely impairs cell growth and viability, induces ER stress responses, disrupts phospholipid biosynthesis, alters membrane morphology, modifies neutral lipid synthesis, and reduces lipid droplet numbers. Overall,LAG1uniquely regulates ER stress and lipid homeostasis, independent of its function in ceramide synthesis. Understanding the specific contributions of LAG1 to lipid homeostasis and ER stress provides valuable insight into cellular stress mechanisms.