bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots
Authors: Chen Chi , Ao Lijiao , Wu Yu‐Tang , Cifliku Vjona , Cardoso Dos Santos Marcelina , Bourrier Emmanuel , Delbianco Martina , Parker David , Zwier Jurriaan M. , Huang Liang , Hildebrandt Niko 
Published: August 2018

AbstractFluorescence barcoding based on nanoparticles provides many advantages for multiparameter imaging. However, creating different concentration‐independent codes without mixing various nanoparticles and by using single‐wavelength excitation and emission for multiplexed cellular imaging is extremely challenging. Herein, we report the development of quantum dots (QDs) with two different SiO2 shell thicknesses (6 and 12 nm) that are coated with two different lanthanide complexes (Tb and Eu). FRET from the Tb or Eu donors to the QD acceptors resulted in four distinct photoluminescence (PL) decays, which were encoded by simple time‐gated (TG) PL intensity detection in three individual temporal detection windows. The well‐defined single‐nanoparticle codes were used for live cell imaging and a one‐measurement distinction of four different cells in a single field of view. This single‐color barcoding strategy opens new opportunities for multiplexed labeling and tracking of cells.
Fabrication and properties of PVDF and PVDF‐HFP microfiltration membranes
Authors: Wang Xinya , Xiao Changfa , Liu Hailiang , Huang Qinglin , Fu Hao 
Published: August 2018

ABSTRACTPoly(vinylidene fluoride) (PVDF) and poly(vinylidene fluoride)‐co‐hexafluoropropylene (PVDF‐HFP) were used to fabricate porous microfiltration membranes via a thermally induced phase separation (TIPS) method, and dibutyl phthalate (DBP) was used as diluent. The effects of polymer concentration on structure and performance were studied in detail. In addition, the effect of incorporation of hexafluoropropylene (HFP) groups on the membrane was also investigated. The formation mechanism was proposed with the assistance of a phase diagram. The results showed that the incorporation of HFP groups resulted in a lower crystallization temperature (Tc) of the polymer/DBP system. In addition, the porosity, pure water flux, and ink solution flux decreased with increasing polymer concentration. In contrast, the water contact angle, ink rejection ratio, and mechanical properties had an increasing tendency. When the polymer concentration was 30 wt %, the obtained membrane was most suitable for microfiltration. Furthermore, the incorporation of HFP groups improved the properties of the obtained membrane, including better hydrophobicity, mechanical properties, antifouling property, and chemical resistance. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46711.
Tackling slip effects in the nonlinear flow properties of gellan fluid gels
Authors: García Mª Carmen , Sánchez Sergio , Trujillo‐Cayado Luis A. , Muñoz José , Alfaro Mª Carmen 
Published: August 2018

ABSTRACTGellan gum is a biopolymer widely used in the food, pharmaceutical, chemical, and agrochemical fields. Its ability to form a strong gel makes it possible to produce fluid gels. These materials present an apparent yield stress, but its value could be influenced by the wall‐slip effect when performing the rheological measurements by which it is determined. In this work, the influence of the measuring surface and gap on flow behavior was first determined. The tests revealed the need to use geometries with rough surfaces, although the sample thickness using a parallel plate has no influence. Subsequently, the value of yield stress was obtained by means of creep tests (found to be 4.3 Pa), and, finally, the effect of wall slip on the dynamic viscoelastic behavior was assessed. There was an influence on the extension of the linear viscoelastic region, but not on the viscoelastic functions of the mechanical spectra. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 46900.
Influence of Disease Activity in Rheumatoid Arthritis on Radiographic Progression of Concomitant Interphalangeal Joint Osteoarthritis
Authors: Lechtenboehmer Christian A. , Jaeger Veronika K. , Kyburz Diego , Walker Ulrich A. , Hügle Thomas 
Published: August 2018

ObjectiveDistal interphalangeal (DIP) joints are commonly considered to be unaffected by rheumatoid arthritis (RA). Despite synovitis and bone marrow edema being associated with radiographic progression in hand osteoarthritis (OA) and hand RA, radiographic courses differ substantially. This study was undertaken to analyze incidence and progression of radiographically evident DIP joint OA in RA patients, in relation to RA activity and patient characteristics.MethodsIn sequential radiographs of 1,988 RA patients in the Swiss Clinical Quality Management in Rheumatic Diseases registry, we evaluated and scored 15,904 DIP joints. Scoring was based on the presence of central erosions and subchondral sclerosis and on the severity of osteophytes and joint space narrowing, according to the modified Kellgren/Lawrence (K/L) grade. The presence of DIP joint OA was defined as ≥1 joint with a K/L grade of ≥2, and progression was defined as an increase in a summed K/L grade. Adjusted odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated.ResultsThe median follow‐up time was 4.5 years (interquartile range 3.1–7.0), and the mean ± SD age was 56.1 ± 11.1 years. DIP joint OA was present in 60% of patients at baseline. Higher mean age (OR 1.09 [95% CI 1.08–1.10]), female sex (OR 1.37 [95% CI 1.08–1.74]), and higher mean body mass index (OR 1.03 [95% CI 1.00–1.06]) were associated with the presence of DIP joint OA, but neither the presence of anti–citrullinated protein antibodies (ACPAs) (OR 0.72 [95% CI 0.50–1.03]) nor the presence of rheumatoid factor (OR 1.01 [95% CI 0.74–1.38]) were associated with it. Disease Activity Score using the erythrocyte sedimentation rate and metacarpophalangeal (MCP) joint erosions were not associated with DIP joint OA progression. RA disease duration had no relevant effect size associated with DIP joint OA progression (OR 0.97 [95% CI 0.96–0.99]).ConclusionKnown risk factors for DIP joint OA were replicated in patients with RA. The observation that RA activity, the presence of ACPA, and MCP joint erosions were not associated with the prevalence or progression of DIP joint OA indicates that there are distinct roles of inflammation in the pathogenesis of RA and DIP joint OA.
Blinded and unblinded sample size reestimation in crossover trials balanced for period
Authors: Grayling Michael J. , Mander Adrian P. , Wason James M. S. 
Published: August 2018

AbstractThe determination of the sample size required by a crossover trial typically depends on the specification of one or more variance components. Uncertainty about the value of these parameters at the design stage means that there is often a risk a trial may be under‐ or overpowered. For many study designs, this problem has been addressed by considering adaptive design methodology that allows for the re‐estimation of the required sample size during a trial. Here, we propose and compare several approaches for this in multitreatment crossover trials. Specifically, regulators favor reestimation procedures to maintain the blinding of the treatment allocations. We therefore develop blinded estimators for the within and between person variances, following simple or block randomization. We demonstrate that, provided an equal number of patients are allocated to sequences that are balanced for period, the proposed estimators following block randomization are unbiased. We further provide a formula for the bias of the estimators following simple randomization. The performance of these procedures, along with that of an unblinded approach, is then examined utilizing three motivating examples, including one based on a recently completed four‐treatment four‐period crossover trial. Simulation results show that the performance of the proposed blinded procedures is in many cases similar to that of the unblinded approach, and thus they are an attractive alternative.
Mechanoluminescence of Ce/Tb inorganic salts in methane–acetylene mixtures with inert gases
Authors: Tukhbatullin Adis A. , Sharipov Glyus L. , Galina Aygul A. 
Published: August 2018

AbstractThe mechanoluminescence of cerium (Ce) and terbium (Tb) lanthanide salts is studied in hydrocarbon [methane (CH4) and acetylene (C2H2)] and inert [helium (He), neon (Ne), argon (Ar), krypton (Kr), and xenon (Xe)] gaseous mixtures. The lines of *N2, *Ln3+, inert gases, *CH, and *C2 radicals resulted from the mechanochemical decomposition of CH4 and C2H2 are observed in the emission spectrum. The luminescence intensity of the inert gases decreases with the hydrocarbon gas concentration in the mixture. The intensities of the *CH or *C2 bands remains almost unchanged within 15–100 vol% of CH4 or C2H2 in the mixture. When the concentration of CH4 or C2H2 is lower than 15%, the intensities of the CH or C2 bands increase achieving their maxima at 0.5–3% of the hydrocarbon. This is probably due to the optimal compositions of the mixtures with the most efficient generation of electrical discharges responsible for mechanoluminescence.
Progression of continuous downstream processing of monoclonal antibodies: Current trends and challenges
Authors: Somasundaram Balaji , Pleitt Kristina , Shave Evan , Baker Kym , Lua Linda H. L. 
Published: August 2018

AbstractRapid advances in intensifying upstream processes for biologics production have left downstream processing as a bottleneck in the manufacturing scheme. Biomanufacturers are pursuing continuous downstream process development to increase efficiency and flexibility, reduce footprint and cost of goods, and improve product consistency and quality. Even after successful laboratory trials, the implementation of a continuous process at manufacturing scale is not easy to achieve. This paper reviews specific challenges in converting each downstream unit operation to a continuous mode. Key elements of developing practical strategies for overcoming these challenges are detailed. These include equipment valve complexity, favorable column aspect ratio, protein‐A resin selection, quantitative assessment of chromatogram peak size and shape, holistic process characterization approach, and a customized process economic evaluation. Overall, this study provides a comprehensive review of current trends and the path forward for implementing continuous downstream processing at the manufacturing scale.
Development of a method for the determination of hydromorphone in plasma by LC–MS
Authors: Cox Sherry , Bergman Joan , Hawkins Shawna , Sladky Kurt 
Published: August 2018

AbstractA simple high‐performance liquid chromatography method for the determination of hydromorphone in small volume plasma has been developed. Following solid‐phase extraction using Oasis HLB cartridges, samples were separated by reverse‐phase high‐performance liquid chromatography on an Atlantis T3 4.6 × 150 mm column (3.0 μm) and quantified using mass spectrometry. The mobile phase was a mixture of water with 0.1% formic acid and acetonitrile with 0.1% formic acid (91:9). The standard curve ranged from 1 to 500 ng/mL. Intra‐ and Inter‐assay variability for hydromorphone was <10%, and the average recovery was >90%. The LLOQ was 1 ng/mL. This method was successfully applied to the analysis of hydromorphone samples at this institution. This method could be useful to those investigators dealing with small sample volumes, particularly when conducting pharmacokinetic studies that require multiple sampling from the same animal.
Applications of decellularized extracellular matrix in bone and cartilage tissue engineering
Authors: Kim Yu Seon , Majid Marjan , Melchiorri Anthony J. , Mikos Antonios G. 
Published: August 2018

AbstractRegenerative therapies for bone and cartilage injuries are currently unable to replicate the complex microenvironment of native tissue. There are many tissue engineering approaches attempting to address this issue through the use of synthetic materials. Although synthetic materials can be modified to simulate the mechanical and biochemical properties of the cell microenvironment, they do not mimic in full the multitude of interactions that take place within tissue. Decellularized extracellular matrix (dECM) has been established as a biomaterial that preserves a tissue's native environment, promotes cell proliferation, and provides cues for cell differentiation. The potential of dECM as a therapeutic agent is rising, but there are many limitations of dECM restricting its use. This review discusses the recent progress in the utilization of bone and cartilage dECM through applications as scaffolds, particles, and supplementary factors in bone and cartilage tissue engineering.
The efficacy of Stereotactic body radiation therapy and the impact of systemic treatments in oligometastatic patients from prostate cancer
Authors: Franzese Ciro , Zucali Paolo Andrea , Di Brina Lucia , D'Agostino Giuseppe , Navarria Pierina , Franceschini Davide , Santoro Armando , Scorsetti Marta 
Published: August 2018

AbstractBackgroundDiagnoses of oligometastatic prostate cancer (PC) increased in the recent years thanks to the advancement in imaging and more effective systemic therapies. Here we evaluate the efficacy of Stereotactic Body Radiation Therapy (SBRT) in oligorecurrent and oligoprogressive PC.MethodsWe included patients with a maximum of five metastases diagnosed in a maximum of two target organs. Concomitant treatment with hormonal therapies or chemotherapies was allowed. End points of the present study were the outcome in terms of Local control of treated metastases (LC), out‐field progression free survival, overall progression free survival (PFS), and overall survival.ResultsWe included in the analysis 64 patients treated on 90 metastases. Fifty (78.1%) patients were treated on lymph nodes, 2 (3.1%) patients simultaneously on lymph node and bone while 10 (15.7%) patients on bone only. Lung metastases were treated in 2 (3.1%) patients. Thirty‐seven (57.81%) were without androgen deprivation therapy when treated with SBRT. Median follow‐up was 15.2 months. Rates of LC at 6‐, 12‐, and 18‐ months were 94%, 88%, and 84%, respectively. Oligoprogressive patients compared to oligorecurrent (HR 9.10, P = 0.049) and prolongation of time from diagnosis of metastases to SBRT (HR 1.03, P = 0.047) were associated with worse LC. Median PFS was 6.6 months (range 1.1‐42.4). Castration resistant patients experienced worse PFS compared to castration sensitive group (HR 2.12, P = 0.021).ConclusionsStereotactic body radiation therapy seems to be an effective treatment for metastases from PC. Prospective trials are necessary to better define selection of patients and to evaluate combination of SBRT and new systemic drugs in castration resistant patients.
Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy
Authors: Das Manabendra , Du Yang , Mortensen Jonas S. , Hariharan Parameswaran , Lee Hyun Sung , Byrne Bernadette , Loland Claus J. , Guan Lan , Kobilka Brian K. , Chae Pil Seok 
Published: August 2018

AbstractA new family of tandem facial glucosides/maltosides (TFGs/TFMs) for membrane protein manipulation was prepared. The best detergent varied depending on the hydrophobic thickness of the target protein, but ether‐based TFMs (TFM‐C0E, TFM‐C3E, and TFM‐C5E) were notable for their ability to confer higher membrane protein stability than the previously developed amide‐based TFA‐1 (P. S. Chae, K. Gotfryd, J. Pacyna, L. J. W. Miercke, S. G. F. Rasmussen, R. A. Robbins, R. R. Rana, C. J. Loland, B. Kobilka, R. Stroud, B. Byrne, U. Gether, S. H. Gellman, J. Am. Chem. Soc. 2010, 132, 16750–16752). Thus, this study not only introduces novel agents with the potential to be used in membrane protein research but also highlights the importance of both the hydrophobic length and linker functionality of the detergent in stabilizing membrane proteins.
Clinical correlation of apoptosis in human granulosa cells—A review
Authors: Almeida Camila P. , Ferreira Márcia C.F. , Silveira Camila O. , Campos Jacira R. , Borges Isabella T. , Baeta Pedro G. , Silva Felipe H.S. , Reis Fernando M. , Del Puerto Helen L. 
Published: August 2018

AbstractThe quality of oocytes depends on interactions with surrounding granulosa cells. Granulosa cells are essential in normal follicular maturation process since they produce steroidal hormones and growth factors, and they play a crucial role in follicular atresia. The success in reproductive biology and medicine depends on reliable assessment of oocyte and embryo viability which presently mainly bases on oocyte and embryo morphology. Recent investigations have tried to establish an evaluation system for oocyte quality and to predict outcome of in vitro fertilization (IVF) based on the incidence of granulosa cells and cumulus cells apoptosis. Apoptosis of granulosa cells seems to have a negative effect on conception and pregnancy rates in IFV programs. Thus, in this review we present a brief outline of clinical correlation of apoptosis in human granulosa cells and cumulus cells, and its influence upon oocyte quality and IFV outcome. Taken together, understanding the influence of granulosa cell apoptosis on oocyte quality and maturity as well as on embryo health may ultimately allow scientists and clinicians to harness better protocols of ovarian stimulation for infertility treatments.
Safety and clinical performance of a drug eluting absorbable metal scaffold in the treatment of subjects with de novo lesions in native coronary arteries: Pooled 12‐month outcomes of BIOSOLVE‐II and BIOSOLVE‐III
Authors: Haude Michael , Ince Hüseyin , Kische Stephan , Abizaid Alexandre , Tölg Ralph , Alves Lemos Pedro , Van Mieghem Nicolas M. , Verheye Stefan , von Birgelen Clemens , Christiansen Evald Høj , Barbato Emanuele , Garcia‐Garcia Hector M. , Waksman Ron ,   on behalf of the BIOSOLVE‐II and III investigators
Published: August 2018

AbstractObjectivesBased on outcomes of the BIOSOLVE‐II study, a novel second generation drug‐eluting absorbable metal scaffold gained CE‐mark in 2016. The BIOSOLVE‐III study aimed to confirm these outcomes and to obtain additional 12‐month angiographic data.BackgroundBioresorbable scaffolds are intended to overcome possible long‐term effects of permanent stents such as chronic vessel wall inflammation, stent crushing, and fractures.MethodsThe prospective, multicenter BIOSOLVE‐II and BIOSOLVE‐III studies enrolled 184 patients with 189 lesions (123 patients in BIOSOLVE‐II and 61 patients in BIOSOLVE‐III). Primary endpoints were in‐segment late lumen loss at 6 months (BIOSOLVE‐II) and procedural success (BIOSOLVE‐III).ResultsMean patient age was 65.5 ± 10.8 years and mean lesion reference diameter was 2.70 ± 0.43 mm. In BIOSOLVE‐III, there were significantly more type B2/C lesions than in BIOSOLVE‐II (80.3% versus 43.4%, P < 0.0001) and significantly more moderate‐to‐severe calcifications (24.2% versus 10.7%, P = 0.014). At 12 months, there was no difference in late lumen loss between the two studies; in the overall population, it was 0.25 ± 0.31 mm in‐segment and 0.39 ± 0.34 mm in‐scaffold. Target lesion failure occurred in six patients (3.3%) and included two cardiac deaths, one target‐vessel myocardial infarction, and three clinically driven target lesion revascularizations. No definite or probable scaffold thrombosis was observed.ConclusionThe pooled outcomes of BIOSOLVE‐II and BIOSOLVE‐III provide further evidence on the safety and performance of a novel drug‐eluting absorbable metal scaffold with constant clinical and angiographic performance parameters at 12 months and no definite or probable scaffold thrombosis.
Don't worry and measure FFR
Authors: Ahn Jung‐Min , Park Seung‐Jung 
Published: August 2018

KEY POINTS

FFR‐guided PCI was associated with better clinical outcomes regarding the reduction of myocardial infarction and major adverse cardiac events.
The clinical benefit of FFR‐guided PCI was achieved with less stents and procedural cost, without additional effects on procedural time and contrast volume.
Physicians should measure FFR of target stenosis prior to PCI if there is no objective evidence of myocardial ischemia.
Precision percutaneous coronary intervention: Is optical coherence tomography co‐registration the future?
Authors: Shlofmitz Evan , Jeremias Allen 
Published: August 2018

KEY POINTS

Integration of angiographic co‐registration (ACR) with optical coherence tomography (OCT) leads to significant changes in PCI strategy.
ACR with OCT allows complete integration of all available data‐points to aid decision making.
Randomized clinical trials comprehensively evaluating the impact of OCT with ACR to improve clinical outcomes are ongoing.
Patent foramen ovale closure for cryptogenic stroke: Effective, but only in select patients
Authors: Chatterjee Arka , Hillegass William B. 
Published: August 2018

Key Points

PFO closure reduces risk of recurrent stroke in some patients with cryptogenic stroke, particularly younger patients <45 years with moderate or greater right to left shunting
Number needed to treat (NNT) to prevent one stroke at 5 years are 28 to 69 (95% confidence limits of 21 to 351) suggesting need for individualized risk stratification models
PFO closure may increase short‐ and possibly long‐term risk of atrial flutter/fibrillation
Hemophilia in the cath lab: Balancing the need to clot with the treatment of thrombosis
Authors: Loloi Justin , Gilchrist Ian C. 
Published: August 2018

KEY POINTS

Hemophilia is a rare genetic disorder that inherently results in bleeding risks that can be successfully managed in the cardiac catheterization laboratory.
Standard of care options such as drug‐coated stents should not be denied hemophiliacs solely on the basis of their genetic defect.
Successful care of hemophilia requires a close collaboration with a specialist in these bleeding disorders to optimize care.
Mortality prediction after transcatheter treatment of failed bioprosthetic aortic valves utilizing various international scoring systems: Insights from the Valve‐in‐Valve International Data (VIVID)
Authors: Aziz Mina , Simonato Matheus , Webb John G. , Abdel‐Wahab Mohamed , McElhinney Doff , Duncan Alison , Tchetche Didier , Barbanti Marco , Petronio Anna Sonia , Maisano Francesco , Ribeiro Vasco Gama , Gaia Diego Felipe , Rana Ruhina , Kocka Viktor , Mathur Moses , Wijeysundera Harindra , Hellig Farrel , Nissen Henrik , Bekeredjian Raffi , Rihal Charanjit , Duffy Stephen J. , Dvir Danny 
Published: August 2018

AbstractBackgroundTranscatheter Aortic Valve Implantation (TAVI) is commonly used to deploy new bioprosthetic valves inside degenerated surgically implanted aortic valves in high risk patients. The three scoring systems used to assess risk of postprocedural mortality are: Logistic EuroSCORE (LES), EuroSCORE II (ES II), and Society of Thoracic Surgeons (STS).ObjectiveThe purpose of this study is to analyze the accuracy of LES, ES II, and STS in estimating all‐cause mortality after transcatheter aortic valve‐in‐valve (ViV) implantations, which was not assessed before.MethodsUsing the Valve‐in‐Valve International Data (VIVID) registry, a total of 1,550 patients from 110 centers were included. The study compared the observed 30‐day overall mortality vs. the respective predicted mortalities calculated by risk scores. The accuracy of prediction models was assessed based on calibration and discrimination.ResultsObserved mortality at 30 days was 5.3%, while average expected mortalities by LES, ES II and STS were 29.49 (± 17.2), 14.59 (± 8.6), and 9.61 (± 8.51), respectively. All three risk scores overestimated 30‐day mortality with ratios of 0.176 (95% CI 0.138–0.214), 0.342 (95% CI 0.264–0.419), and 0.536 (95% CI 0.421–0.651), respectively. 30‐day mortality ROC curves demonstrated that ES II had the largest AUC at 0.722, followed by STS at 0.704, and LES at 0.698.ConclusionsAll three scores overestimated mortality at 30 days with ES II showing the highest predictability compared to LES and STS; and therefore, should be recommended for ViV procedures. There is a need for a dedicated scoring system for patients undergoing ViV interventions.