bluuebunny/crossref_metadata_2025_split huggingface dataset:
The sero‐epidemiology of Coxiella burnetii (Q fever) across livestock species and herding contexts in Laikipia County, Kenya
Authors: Larson Peter S. , Espira Leon , Grabow Cole , Wang Christine A. , Muloi Dishon , Browne A. Springer , Deem Sharon L. , Fèvre Eric M. , Foufopoulos Johannes , Hardin Rebecca , Eisenberg Joseph N. S. 
Published: February 2019

AbstractCoxiella burnetii, the causative agent of Query fever (Q fever), is among the most highly infectious zoonotic pathogens transmitted among livestock, with chronic effects challenging to veterinary and medical detection and care systems. Transmission among domestic livestock species can vary regionally due to herd management practices that determine which livestock species are raised, whether or not livestock are in contact with wildlife, and the susceptibility of these livestock to infection. To explore how different livestock management practices are associated with the risk of infection in multispecies environments, we carried out a comparative study of three types of herd management systems in the central Kenyan county of Laikipia: agro‐commercial, mixed conservancy/commercial, and smallholder ranches. We tested C. burnetii antibody seroprevalence in four common livestock species. Across all management types, the highest seroprevalence was in camels (20%), followed by goats (18%), sheep (13%), and cattle (6%). We observed a lower odds of testing seropositive for young compared to adult animals (adjusted OR = 0.44 [95% CI 0.24, 0.76]), and for males compared to females (adjusted OR = 0.52 [95% CI 0.33, 0.80]). Animals from mixed conservancy/commercial and smallholder operations had a higher odds of testing seropositive compared to animals from agro‐commercial ranches (adjusted OR = 5.17 [95% CI 2.71, 10.44] and adjusted OR = 2.21 [95% CI 1.17, 4.43] respectively). These data suggest that herd management practices might affect the transmission dynamics of C. burnetiiin arid African ecosystems like those seen in Kenya where several transmission modes are possible, risk of drought has promoted new livestock species such as camels, and multiple wildlife species may co‐occur with livestock on the landscape. Further longitudinal studies are needed to disentangle the mechanisms underlying these patterns, and further explore transmission patterns between wildlife, domestic animal, and human populations.
On the importance of fine‐scale sampling in detecting alpha taxonomic diversity among saproxylic invertebrates: A velvet worm (Onychophora: Opisthopatus amaxhosa) template
Authors: Barnes Aaron , Daniels Savel R. 
Published: February 2019

AbstractThe phylogeography and social structure of the narrow endemic velvet worm species Opisthopatus amaxhosa were investigated by conducting fine‐scale sampling in its distribution range in the Eastern Cape province of South Africa. In addition, and as part of larger grant on forest biodiversity, Opisthopatus specimens sampled at localities not included during a recent evaluation of the genus were included in a new phylogeny. A total of 89 specimens from 18 sample localities were collected at three forest patches for O. amaxhosa samples, while an additional six Opisthopatus sample localities were included. For O. amaxhosa, we sequenced the COI locus for all specimens, while a subset of specimens was sequenced for two nuclear loci, 18S rRNA and the fushi tarazu intron (FTz). Phylogenetic analyses using maximum likelihood and Bayesian inferences of the latter species revealed the presence of two highly divergent clades, characterised by marked uncorrected sequence divergence values. In addition, these two clades did not share any maternal haplotypes, were characterised by high FST values and fixed nuclear difference for the 18S rRNA locus, while the FTz intron was genetically invariant. Furthermore, the application of scanning electron microscopy between the two genetically divergent clades also revealed the presence of fixed ventral and dorsal scale numbers. Collectively, this provides evidence for a novel species that is present at a fine scale. Divergence time estimations suggest that the two clades diverged during the late and early Pleistocene with climatic cycling potentially causal to the fragmentation. The social structure was male‐biased, and samples from the same logs were not always genetically identical. At the broader scale, the inclusion of new specimens within Opisthopatus revealed no novel lineages. Fine‐scale sampling appears more important to detect alpha taxonomic diversity compared to broadscale sampling.
Patient relevant outcomes of unicompartmental versus total knee replacement: systematic review and meta-analysis
Authors: Wilson Hannah A , Middleton Rob , Abram Simon G F , Smith Stephanie , Alvand Abtin , Jackson William F , Bottomley Nicholas , Hopewell Sally , Price Andrew J 
Published: February 2019

AbstractObjectiveTo present a clear and comprehensive summary of the published data on unicompartmental knee replacement (UKA) or total knee replacement (TKA), comparing domains of outcome that have been shown to be important to patients and clinicians to allow informed decision making.DesignSystematic review using data from randomised controlled trials, nationwide databases or joint registries, and large cohort studies.Data sourcesMedline, Embase, Cochrane Controlled Register of Trials (CENTRAL), and Clinical Trials.gov, searched between 1 January 1997 and 31 December 2018.Eligibility criteria for selecting studiesStudies published in the past 20 years, comparing outcomes of primary UKA with TKA in adult patients. Studies were excluded if they involved fewer than 50 participants, or if translation into English was not available.Results60 eligible studies were separated into three methodological groups: seven publications from six randomised controlled trials, 17 national joint registries and national database studies, and 36 cohort studies. Results for each domain of outcome varied depending on the level of data, and findings were not always significant. Analysis of the three groups of studies showed significantly shorter hospital stays after UKA than after TKA (−1.20 days (95% confidence interval −1.67 to −0.73), −1.43 (−1.53 to −1.33), and −1.73 (−2.30 to −1.16), respectively). There was no significant difference in pain, based on patient reported outcome measures (PROMs), but significantly better functional PROM scores for UKA than for TKA in both non-trial groups (standard mean difference −0.58 (−0.88 to −0.27) and −0.29 (−0.46 to −0.11), respectively). Regarding major complications, trials and cohort studies had non-significant results, but mortality after TKA was significantly higher in registry and large database studies (risk ratio 0.27 (0.16 to 0.45)), as were venous thromboembolic events (0.39 (0.27 to 0.57)) and major cardiac events (0.22 (0.06 to 0.86)). Early reoperation for any reason was higher after TKA than after UKA, but revision rates at five years remained higher for UKA in all three study groups (risk ratio 5.95 (1.29 to 27.59), 2.50 (1.77 to 3.54), and 3.13 (1.89 to 5.17), respectively).ConclusionsTKA and UKA are both viable options for the treatment of isolated unicompartmental osteoarthritis. By directly comparing the two treatments, this study demonstrates better results for UKA in several outcome domains. However, the risk of revision surgery was lower for TKA. This information should be available to patients as part of the shared decision making process in choosing treatment options.Systematic review registrationPROSPERO number CRD42018089972.
NETWORK ANALYSIS OF COMMON DIFFERENTIAL GENES IDENTIFIES KEY GENES AND IMPORTANT MODULES UNDERLYING EXTRACELLULAR ELECTRON TRANSFER PROCESSES
Authors: DING DEWU 
Published: February 2019

Electricigens can transfer electrons that produced in intracellular metabolic processes to cellular surface to restore extracellular insoluble electron acceptors (extracellular electron transfer, EET). To uncover the molecular mechanisms underlying EET processes, we integrated transcriptome changes accompanying such processes with molecular network. Firstly, time-series expression datasets for Shewanella oneidensis MR-1 under limited/changed [Formula: see text] conditions were obtained from the GEO database, and a total of 336 common differentially expressed genes (DEGs) were identified. Then, we constructed the protein–protein interaction (PPI) network that involved in EET processes from these DEGs. Furthermore, by using centralization analysis and community detection, network analysis of the PPI network was performed. Although the fundamental EET genes are similar to previous studies, important new genes have been discovered. Taking together, our study identified many literature-validated genes critical to EET processes, and also proposed some novel genes that were putatively involved in EET processes.
90-РІЧЧЮ КІРОВОГРАДСЬКОГО МЕДИЧНОГО КОЛЕДЖУ ІМ. Є.Й. МУХІНА ПРИСВЯЧУЄТЬСЯ
Authors: Goot T. M. 
Published: February 2019

У статті наведено основні питання, що розглядались на регіональній науково-практичній конференції в рамках підготовки до ювілею навчального закладу стосовно паліативної та хоспісної допомоги інкурабельним хворим.
Hematopoietic ChemR23 (Chemerin Receptor 23) Fuels Atherosclerosis by Sustaining an M1 Macrophage-Phenotype and Guidance of Plasmacytoid Dendritic Cells to Murine Lesions—Brief Report
Authors: van der Vorst Emiel P.C. , Mandl Manuela , Müller Madeleine , Neideck Carlos , Jansen Yvonne , Hristov Michael , Gencer Selin , Peters Linsey J.F. , Meiler Svenja , Feld Micha , Geiselhöringer Anna-Lena , de Jong Renske J. , Ohnmacht Caspar , Noels Heidi , Soehnlein Oliver , Drechsler Maik , Weber Christian , Döring Yvonne 
Published: February 2019

Objective—
Expression of the chemokine-like receptor ChemR23 (chemerin receptor 23) has been specifically attributed to plasmacytoid dendritic cells (pDCs) and macrophages and ChemR23 has been suggested to mediate an inflammatory immune response in these cells. Because chemokine receptors are important in perpetuating chronic inflammation, we aimed to establish the role of ChemR23-deficiency on macrophages and pDCs in atherosclerosis.

Approach and Results—

ChemR23-knockout/knockin mice expressing eGFP (enhanced green fluorescent protein) were generated and after crossing with apolipoprotein E-deficient (
Apoe
−/−
ChemR23

e/e

) animals were fed a western-type diet for 4 and 12 weeks.
Apoe
−/−
ChemR23

e/e

mice displayed reduced lesion formation and reduced leukocyte adhesion to the vessel wall after 4 weeks, as well as diminished plaque growth, a decreased number of lesional macrophages with an increased proportion of M2 cells and a less inflammatory lesion composition after 12 weeks of western-type diet feeding. Hematopoietic ChemR23-deficiency similarly reduced atherosclerosis. Additional experiments revealed that ChemR23-deficiency induces an alternatively activated macrophage phenotype, an increased cholesterol efflux and a systemic reduction in pDC frequencies. Consequently, expression of the pDC marker SiglecH in atherosclerotic plaques of
Apoe
−/−
ChemR23

e/e

mice was declined. ChemR23-knockout pDCs also exhibited a reduced migratory capacity and decreased CCR (CC-type chemokine receptor)7 expression. Finally, adoptive transfer of sorted wild-type and knockout pDCs into
Apoe
−/−
recipient mice revealed reduced accumulation of ChemR23-deficient pDCs in atherosclerotic lesions.

Conclusions—
Hematopoietic ChemR23-deficiency increases the proportion of alternatively activated M2 macrophages in atherosclerotic lesions and attenuates pDC homing to lymphatic organs and recruitment to atherosclerotic lesions, which synergistically restricts atherosclerotic plaque formation and progression.
Observations of Turbulent Heat and Momentum Fluxes during Wildland Fires in Forested Environments
Authors: Heilman Warren E. , Bian Xindi , Clark Kenneth L. , Zhong Shiyuan 
Published: February 2019

AbstractTurbulent fluxes of heat and momentum in the vicinity of wildland fires contribute to the redistribution of heat and momentum in the fire environment, which in turn can affect the heating of fuels, fire behavior, and smoke dispersion. As an extension of previous observational studies of turbulence regimes in the vicinity of wildland fires in forested environments, this study examines the effects of spreading surface fires and forest overstory vegetation on turbulent heat and momentum fluxes from near the surface to near the top of the overstory vegetation. Profiles of high-frequency (10 Hz) wind velocity and temperature measurements during two prescribed fire experiments are used to assess the relative contributions of horizontal and vertical turbulent fluxes of heat and momentum to the total heat and momentum flux fields. The frequency-dependent temporal variability of the turbulent heat and momentum fluxes before, during, and after fire-front passage is also examined using cospectral analyses. The study results highlight the effects that surface wildland fires and forest overstory vegetation collectively can have on the temporal and vertical variability of turbulent heat and momentum fluxes in the vicinity of the fires and the substantial departures of heat and momentum cospectra from typical atmospheric surface-layer cospectra that can occur before, during, and after fire-front passage.
Monitoring of Drought Awareness from Google Trends: A Case Study of the 2011–17 California Drought
Authors: Kam Jonghun , Stowers Kimberly , Kim Sungyoon 
Published: February 2019

Abstract
This study introduces “Google Trends” as a social data source in monitoring and modeling the dynamics of drought awareness during the 2011–17 California drought. In this study, drought awareness is defined and operationalized as the relative search interest activities within California, using the search term “drought” from Google Trends. First, the 2011–17 California drought is characterized in the duration–intensity curve with other historical California droughts for comparative purposes, using the 12-month standard precipitation index data (1895–2017). Second, the potential triggers of the peaks of drought awareness during the 2011–17 California drought are investigated through Google Trends and Google Search. The Google Trends data show that the first peak of drought awareness occurred when the drought condition reached its peak and the governor declared the drought emergency (January 2014). The other peaks in August 2014, April 2015, and January 2017 are related to public interest in drought recovery driven by the forecast of the strong El Niño winter of 2014/15, the governor’s issue of water use rules, and California floods in early 2017, respectively. Last, a power-law decay model of drought awareness is fitted to the Google Trends data. According to the fitted power-law model, Californians remained interested in drought after the social trigger–related peaks longer than they did after the natural trigger–related peaks. The findings of this study suggest that it is necessary to develop a more realistic social dynamic modeling for communities that can respond to natural and human triggers and capture interactions with awareness of related disasters.
Cross state-dependency of learning between 5-HT1A and/or 5-HT7 receptor agonists and muscimol in the mouse dorsal hippocampus
Authors: Jafari-Sabet Majid , Nemati Sepehr , Torab Mansour 
Published: February 2019

Background: Dysfunction of the serotonergic and GABAergic systems in cognitive disorders has been revealed. Understanding the neurobiological mechanisms of drug-associated learning and memory formation may help treatment of cognitive disorders. Aims: The aim of the present study was to investigate: 1) 8-OH-DPAT (5-HT1A agonist), AS19 (5-HT7 agonist) and muscimol (GABA-A agonist) on memory retrieval and state of memory, 2) cross state-dependent learning between 8-OH-DPAT and/or AS19 and muscimol. Methods: The dorsal hippocampal CA1 regions of adult male NMRI mice were bilaterally cannulated, and all drugs were microinjected into the intended sites of injection. A single-trial step-down inhibitory avoidance task was used for the evaluation of memory retrieval and state of memory. Results: Post-training and/or pre-test 8-OH-DPAT, AS19 and muscimol induced amnesia. Pre-test microinjection of the same doses of 8-OH-DPAT, AS19 and muscimol reversed the post-training 8-OH-DPAT-, AS19- and muscimol-induced amnesia, respectively. This event has been named state-dependent learning (SDL). The amnesia induced by 8-OH-DPAT was reversed by muscimol and induced 8-OH-DPAT SDL. The amnesia induced by muscimol was reversed by 8-OH-DPAT and induced muscimol SDL. The amnesia induced by AS19 was reversed by muscimol and induced AS19 SDL. The amnesia induced by muscimol was reversed by AS19 and induced muscimol SDL. Pre-test administration of a selective GABA-A receptor antagonist, bicuculline, 5 min before muscimol, 8-OH-DPAT and AS19 dose-dependently inhibited muscimol-, 8-OH-DPAT- and AS19-induced SDL, respectively. Conclusions: The results strongly revealed a cross SDL among 8-OH-DPAT and/or AS19 and muscimol in the dorsal hippocampal CA1 regions.
Increased cerebral responses to salient transitions between alternating stimuli in chronic migraine with medication overuse headache and during migraine attacks
Authors: Bogdanov Volodymyr B , Bogdanova Olena V , Viganò Alessandro , Noirhomme Quentin , Laureys Steven , Dallel Radhouane , Phillips Christophe , Schoenen Jean 
Published: February 2019

Introduction In a previous study exploring central pain modulation with heterotopic stimuli in healthy volunteers, we found that transitions between sustained noxious and innocuous thermal stimulations on the foot activated the “salience matrix”. Knowing that central sensory processing is abnormal in migraine, we searched in the present study for possible abnormalities of these salient transitional responses in different forms of migraine and at different time points of the migraine cycle. Methods Participants of both sexes, mostly females, took part in a conditioned pain modulation experiment: Migraineurs between (n = 14) and during attacks (n = 5), chronic migraine patients with medication overuse headache (n = 7) and healthy volunteers (n = 24). To evoke the salience response, continuous noxious cold or innocuous warm stimulations were alternatively applied on the right foot. Cerebral blood oxygenation level dependent responses were recorded with fMRI. Results Switching between the two stimulations caused a significant transition response in the “salience matrix” in all subject groups (effect of the condition). Moreover, some group effects appeared on subsequent post-hoc analyses. Augmented transitional blood oxygenation level dependent responses in the motor cortex and superior temporal sulcus were found in two patient groups compared to healthy controls: chronic migraine with medication overuse headache patients and migraineurs recorded during an attack. In chronic migraine with medication overuse headache patients, salience-related responses were moreover greater in the premotor cortex, supplementary motor area, lingual gyrus and dorso-medial prefrontal cortex and other “salience matrix” areas, such as the anterior cingulate and primary somatosensory cortices. Conclusion This study shows salience-related hyperactivation of affective and motor control areas in chronic migraine with medication overuse headache patients and, to a lesser extent, in episodic migraine patients during an attack. The greater extension of exaggerated blood oxygenation level dependent responses to unspecific salient stimuli in chronic migraine with medication overuse headache than during a migraine attack could be relevant for headache chronification.
Safety and tolerability of calcitonin-gene-related peptide binding monoclonal antibodies for the prevention of episodic migraine – a meta-analysis of randomized controlled trials
Authors: Xu Da , Chen Deng , Zhu Li-na , Tan Ge , Wang Hai-jiao , Zhang Yu , Liu Ling 
Published: February 2019

Aim To systematically evaluate the safety and tolerability of calcitonin-gene-related peptide binding monoclonal antibodies from the results of randomized controlled trials. Methods Online databases were searched on calcitonin-gene-related peptide binding monoclonal antibodies for the prevention of episodic migraine. Overall withdrawal, withdrawal due to adverse events, adverse events, serious adverse events and specific adverse events were extracted from the included studies. A meta-analysis was performed with Revman 5.3.0 software. Results Ten studies that investigated four drugs (galcanezumab, erenumab, fremanezumab and eptinezumab) with 5817 participants were included in this study. Serious adverse events, overall withdrawals, withdrawal due to adverse events and any adverse events were not significantly associated with monoclonal antibody treatment. Injection site pain and erythema were significantly higher in the calcitonin-gene-related peptide binding monoclonal antibodies treatment group than in the placebo group. The rates of serious adverse events were significantly higher in the galcanezumab 120 mg group. Injection site erythema was associated with galcanezumab 120 mg and 240 mg. Injection site pain and nasopharyngitis were associated with galcanezumab 150 mg and 5 mg, respectively. Overall adverse events were significantly higher with erenumab 70 mg and 140 mg. Treatment-related adverse events were significantly higher with fremanezumab 225 mg/month and 675 mg/quarter. Conclusions This study provides data on the safety and tolerability profiles of calcitonin-gene-related peptide binding monoclonal antibodies and confirms their potential use as preventive treatments for episodic migraine. In addition to the acceptable withdrawal rates, serious adverse events were rare, and the severity of most adverse events was mild to moderate. Injection site reaction may be the major adverse event associated with galcanezumab.
Incidence, clinical features and outcomes of atrial fibrillation and stroke in Qatar
Authors: Imam Yahia Z , Kamran Saadat , Akhtar Naveed , Deleu Dirk , Singh Rajvir , Malik Rayaz A , Abdelmoneim MS , Bermejo Pablo , Bourke Paula , Morgan Deborah , Santos Mark , Joseph Sujatha , Shuaib Ashfaq 
Published: February 2019

Background Atrial fibrillation is an important risk factor for stroke but there are limited data on atrial fibrillation-related stroke from the Middle East. Methods We interrogated the Qatar Stroke Database to establish the occurrence, clinical features, and outcomes of atrial fibrillation-related stroke at Hamad General Hospital, the sole provider of acute stroke care in Qatar. Results A total of 4079 patients (81.4% male, mean age 55.4 ± 13.3 years) were enrolled in the stroke database between January 2014 and 21 October 2017. Atrial fibrillation was present in 260 (6.4%) patients, of whom 106 (2.6%) had newly diagnosed atrial fibrillation. The National Institute of Health Stroke Scale (NIHSS) was significantly higher (7.9 + 7.0 (median 6; IQR 11) vs. 5.9 + 6.4 (median 4; IQR 6), P < 0.001) in atrial fibrillation patients. The modified Rankin Score (mRS) (P < 0.001) and mortality at 90-day follow-up (P = 0.002) were significantly higher in atrial fibrillation compared to non-atrial fibrillation stroke patients. Conclusion We demonstrate a low rate of atrial fibrillation and stroke in Qatar, perhaps reflecting the relatively young age of these patients. Atrial fibrillation-related strokes had higher admission NIHSS, greater disability, and higher mortality at 90 days when compared to non-atrial fibrillation strokes.