bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physical inactivity, television time and chronic diseases in Brazilian adults and older adults
Authors: Christofoletti Marina , Del Duca Giovani Firpo , da Silva Kelly Samara , Meneghini Vandrize , Malta Deborah de Carvalho 
Published: March 2019

Summary
This study aimed to investigate the association between simultaneity of leisure-time physical inactivity and high television time with the presence of chronic diseases in adults and older adults in Brazil. We analyzed secondary data from Vigitel 2013 database, a cross-sectional national survey. It was included 37 947 adults (18–59 years) and 14 982 older adults (≥60 years) living in Brazilian state capitals. The association was between simultaneity of risk behaviors considering leisure-time physical inactivity (&lt;10 min/day) and high television time (≥2 h/day), with the presence of chronic diseases (diabetes, dyslipidemia, hypertension and obesity), measured according to the age group. We performed a binary logistic regression, crude and adjusted analysis, using weighted to reflect population estimates. In adults, there was an association between leisure-time physical inactivity and television time with the presence of diseases. In practice, the simultaneity of risk behaviors when compared with those without risk behavior had higher odds for diabetes (OR = 1.83), dyslipidemia (OR = 1.17), hypertension (OR = 1.54) and obesity (OR = 1.60). In older adults, the simultaneity was associated with diabetes (OR = 1.61), hypertension (OR = 1.33) and obesity (OR = 1.81). We concluded that for adults and older adults the simultaneity of leisure-time physical inactivity and high television time increased the odds for the presence of chronic disease, especially for diabetes and obesity.
Machine intelligence and the data-driven future of marine science
Authors: Malde Ketil , Handegard Nils Olav , Eikvil Line , Salberg Arnt-Børre 
Published: March 2019

AbstractOceans constitute over 70% of the earth's surface, and the marine environment and ecosystems are central to many global challenges. Not only are the oceans an important source of food and other resources, but they also play a important roles in the earth's climate and provide crucial ecosystem services. To monitor the environment and ensure sustainable exploitation of marine resources, extensive data collection and analysis efforts form the backbone of management programmes on global, regional, or national levels. Technological advances in sensor technology, autonomous platforms, and information and communications technology now allow marine scientists to collect data in larger volumes than ever before. But our capacity for data analysis has not progressed comparably, and the growing discrepancy is becoming a major bottleneck for effective use of the available data, as well as an obstacle to scaling up data collection further. Recent years have seen rapid advances in the fields of artificial intelligence and machine learning, and in particular, so-called deep learning systems are now able to solve complex tasks that previously required human expertise. This technology is directly applicable to many important data analysis problems and it will provide tools that are needed to solve many complex challenges in marine science and resource management. Here we give a brief review of recent developments in deep learning, and highlight the many opportunities and challenges for effective adoption of this technology across the marine sciences.
Self-Defense by Non-State Actors in States of Fragmented Authority
Authors: Tzimas Themis 
Published: March 2019

Abstract
The current article focuses on Events in the Middle East in relation to non-state actors and the right of self-defense. As study-case is adopted for Yemen, where the Houthis, as the de facto authority—and, according to their own standpoint as the de jure authority too—of the state, or at least large parts of it, declare that they exercise a right to self-defense against the Saudi-led intervention.
The theme is examined in the context of protracted conflicts, which from non-international become internationalized armed conflicts, in states of fragmented authority and sovereignty. The argument is that while non-state actors (NSAs) do not autonomously possess a right of their own to self-defense, in cases involving states of fragmented authority and sovereignty they may exercise this right, on behalf of the state which is suffering the attack for as long as the government is not in a position to fulfill its responsibility regarding the defense of the state.
The article does not advocate a contra legem interpretation of Article 51, expanding its application to non-state actors. It argues in favor of the substitution of the entity that is entitled to implement Article 51, when the government of a state is absent or incapable of carrying out its duties. It is in this sense that non-state actors are linked to the right of self-defense.
Financial citizenship and nation-building in Malaysia: elites' and citizens' perspectives
Authors: Abdul Rahman Syahirah , Ertürk Ismail , Froud Julie 
Published: March 2019

AbstractThis article presents a postcolonial analysis of financial citizenship (FC) programmes in Malaysia. Drawing on secondary data and on interviews with elites and citizen investors, the paper explores the spatial and historically specific nature of financialisation in a postcolonial context. Specifically, the paper draws out the significance of FC as part of broader nation building objectives in Malaysia from an elite perspective, while also observing the reluctance of citizen investors who are engaging with the equity market to support the formal objectives of the policy. In doing so, it provides an example of the financialisation of everyday life in a distinctive and complex emerging economy context. Moreover, the paper explores these processes from both elite and citizen perspectives, allowing these layered relations within FC to be analysed. The article, therefore, contributes to the financialisation literature by bringing new understandings of elite–citizen relations in postcolonial nation-building strategies.
Early- and late-stage mergers among main sequence and starburst galaxies at 0.2 ≤ z ≤ 2
Authors: Cibinel A , Daddi E , Sargent M T , Le Floc’h E , Liu D , Bournaud F , Oesch P A , Amram P , Calabrò A , Duc P-A , Pannella M , Puglisi A , Perret V , Elbaz D , Kokorev V 
Published: March 2019

Abstract
We investigate the fraction of close pairs and morphologically identified mergers on and above the star-forming main sequence (MS) at 0.2 ≤ z ≤2.0. The novelty of our work lies in the use of a non-parametric morphological classification performed on resolved stellar mass maps, reducing the contamination by non-interacting, high-redshift clumpy galaxies. We find that the merger fraction rapidly rises to ≥70 per cent above the MS, implying that – already at z ≳ 1 – starburst (SB) events (ΔMS ≥ 0.6) are almost always associated with a major merger (1:1 to 1:6 mass ratio). The majority of interacting galaxies in the SB region are morphologically disturbed, late-stage mergers. Pair fractions show little dependence on MS offset and pairs are more prevalent than late-stage mergers only in the lower half of the MS. In our sample, major mergers on the MS occur with a roughly equal frequency of ∼5–10 per cent at all masses ≳ 1010 M⊙. The MS major merger fraction roughly doubles between z = 0.2 and 2, with morphological mergers driving the overall increase at z ≳ 1. The differential redshift evolution of interacting pairs and morphologically classified mergers on the MS can be reconciled by evolving observability time-scales for both pairs and morphological disturbances. The observed variation of the late-stage merger fraction with ΔMS follows the perturbative 2-Star Formation Mode model, where any MS galaxy can experience a continuum of different star formation rate enhancements. This points to an SB–merger connection not only for extreme events, but also more moderate bursts which merely scatter galaxies upward within the MS, rather than fully elevating them above it.
Is it possible to reconcile extragalactic IMF variations with a universal Milky Way IMF?
Authors: Guszejnov Dávid , Hopkins Philip F , Graus Andrew S 
Published: March 2019

Abstract
One of the most robust observations of the stellar initial mass function (IMF) is its near-universality in the Milky Way and neighbouring galaxies. But recent observations of early-type galaxies can be interpreted to imply a ‘bottom-heavy’ IMF, while others of ultrafaint dwarfs could imply a ‘top-heavy’ IMF. This would impose powerful constraints on star formation models. We explore what sort of ‘cloud-scale’ IMF models could possibly satisfy these constraints. We utilize simulated galaxies that reproduce (broadly) the observed galaxy properties, while they also provide the detailed star formation history and properties of each progenitor star-forming cloud. We then consider generic models where the characteristic mass of the IMF is some arbitrary power-law function of progenitor cloud properties, along with well-known literature IMF models which scale with Jeans mass, ‘turbulent Bonnor–Ebert mass’, temperature, the opacity limit, metallicity, or the ‘protostellar heating mass’. We show that no IMF models currently in the literature – nor any model where the turnover mass is an arbitrary power-law function of a combination of cloud temperature/density/size/metallicity/velocity dispersion/magnetic field – can reproduce the claimed IMF variation in ellipticals or dwarfs without severely violating observational constraints in the Milky Way. Specifically, they predict too much variation in the ‘extreme’ environments of the Galaxy compared to that observed. Either the IMF varies in a more complicated manner, or alternative interpretations of the extragalactic observations must be explored.
Association Between Opioid Use and Patient-Reported Outcomes in a Randomized Trial Evaluating Basivertebral Nerve Ablation for the Relief of Chronic Low Back Pain
Authors: Markman John D , Rhyne Alfred L , Sasso Rick C , Patel Alpesh A , Hsu Wellington K , Fischgrund Jeffrey S , Edidin Avram A , Vajkoczy Peter 
Published: March 2019

Abstract

BACKGROUND
Chronic low back pain (CLBP) is a primary indication for opioid therapy.

OBJECTIVE
To evaluate the hypothesis that CLBP patients reporting reduced opioid use have superior functional outcomes following basivertebral nerve (BVN) radiofrequency ablation.

METHODS
This post hoc analysis from a sham-controlled trial examined short-acting opioid use from baseline through 1 yr. Opioid use was stratified into 3 groups by two blinded external reviewers. Two-sample t-tests were used to compare Oswestry Disability Index (ODI) and Visual Analog Scale (VAS) measurements between those patients who increased or decreased their opioid usage compared to baseline.

RESULTS
Actively treated patients with decreased opioid use at 12 mo had a mean ODI improvement of 24.9 ± 16.0 (n = 27) compared to 7.3 ± 9.8 (n = 18) for patients reporting increased opioid use (P < .001). In the sham arm, the improvements in ODI were 17.4 ± 16.1 (n = 19) and 1.2 ± 14.3 (n = 5; P = .053) for the patients reporting decreased vs increased opioid usage, respectively. Actively treated patients reporting decreased opioid use had a mean improvement in VAS of 3.3 ± 2.5 (n = 27) compared to 0.6 ± 1.8 (n = 18) for patients reporting increased opioid use (P < .001). In the sham arm, the improvements in VAS were 2.5 ± 2.6 (n = 19) and 1.4 ± 1.9 (n = 5; P = .374) for patients reporting decreased vs increased opioid use, respectively.

CONCLUSION
Subjects undergoing BVN ablation who decreased opioid use had greater improvement in ODI and VAS scores compared with those reporting increased opioid usage. There is an association between functional benefit from BVN ablation and reduced opioid use.
Incidental Low-Grade Gliomas: Single-Institution Management Based on Clinical, Surgical, and Molecular Data
Authors: Ius Tamara , Cesselli Daniela , Isola Miriam , Pauletto Giada , Tomasino Barbara , D’Auria Stanislao , Bagatto Daniele , Pegolo Enrico , Beltrami Antonio Paolo , Loreto Carla di , Skrap Miran 
Published: March 2019

Abstract

BACKGROUND
Incidentally discovered diffuse low-grade gliomas (iLGG) are poorly documented in the literature. They are diagnosed by chance during radiological examinations.

OBJECTIVE
To review a cohort of patients with iLGG surgically treated in our institution, analyzing clinical, molecular, and surgical aspects.

METHODS
Clinical, radiological, and treatment data of iLGG were retrieved and compared with those of symptomatic diffuse LGGs (sLGG). Histological and molecular review was carried out as well. The extent of resection was evaluated on preoperative and postoperative T2-weighted magnetic resonance imaging.

RESULTS
Thirty-four iLGG cases were identified within a monoinstitutional cohort of 332 patients operated for low-grade gliomas from 2000 to 2017. Clinically, patients with iLGG had higher preoperative karnofsky performance scale (KPS) (P = .003), smaller tumor volume (P = .0001), lower frequency of eloquent areas involvement (P = .0001), and higher rate of complete resection (P = .0001) compared to those with sLGG. No differences in the molecular profile and O6-methylguanine-DNA-methyltransferase promoter methylation were detected between iLGG and sLGG. Importantly, patients with iLGG had longer overall survival than those with sLGG (P = .0001), even when a complete surgical resection was achieved (P = .001).

CONCLUSION
Although the therapeutic strategy of iLGG is still a matter of debate, our data support the safety and the effectiveness of early surgical resection. The favorable prognosis of iLGG may be due to the higher practicability of extensive resection, noneloquent tumor location, and smaller tumor volume.
Optimization of the navigated TMS mapping algorithm for accurate detection of plasticity and abnormalities in cortical muscle representations
Authors: Sinitsyn Dmitry O. , Chernyavskiy Andrey Yu. , Poydasheva Alexandra G. , Bakulin Ilya S. , Suponeva Natalia A. , Piradov Michael A. 
Published: March 2019

AbstractNavigated TMS mapping of cortical muscle representations allows noninvasive assessment of the state of a healthy or diseased motor system and monitoring its change with time. These applications are hampered by the heterogeneity of existing mapping algorithms and the lack of detailed information about their accuracy. We aimed to find an optimal motor evoked potential (MEP) sampling scheme in the grid-based mapping algorithm in terms of the accuracy of muscle representation parameters. The APB muscles of eight healthy subjects were mapped three times on consecutive days using a seven-by-seven grid with ten stimuli per cell. The effect of the MEP variability on the parameter accuracy was assessed using bootstrapping. The accuracy of representation parameters increased with the number of stimuli without saturation up to at least ten stimuli per cell. The detailed sampling showed that the between-session representation area changes in the absence of interventions were significantly larger than the within-session fluctuations and thus could not be explained solely by the trial-to-trial variability of MEPs. The results demonstrate that the number of stimuli has no universally optimal value and must be chosen by balancing the accuracy requirements with the mapping time constraints in a given problem.
Colocalization of MOR1 and GAD67 in Mouse Nucleus Accumbens
Authors: Hinkle Chad , Dedkov Eduard , Buono Russell , Ferraro Thomas 
Published: March 2019

ABSTRACTCurrent understanding of the rewarding and addictive effects of opioids involves mu-opioid receptor (MOR) binding within the nucleus accumbens (NAcc), a region of the ventral striatum. GABAergic neurotransmission in the NAcc potentiates the rewarding response to opioids, and in fact, drugs that stimulate GABAergic activity are also addictive, a phenomenon mediated in part by endogenous opioids. However, the neuroanatomical relationship between opioid and GABA systems is still unclear, and further study of the interaction between these neurotransmitter systems in the reward pathway is warranted. We report evidence supporting the direct interaction between GABAergic and opioidergic neurotransmitter systems within the mouse NAcc. Male and female FVB/NJ mice (12-16 months of age) were euthanized via carbon dioxide inhalation and brains processed for histology and immunohistochemistry (IHC). Coronal cryosections (10-12 um in thickness) were taken through the NAcc at the level of the anterior commissure. A mouse monoclonal antibody against GAD67, an enzyme catalyzing GABA production, was used in conjunction with an anti-mouse rhodamine red-X-labeled secondary antibody to identify GABAergic neurons in the NAcc. Alternating sections were stained for MOR using a fluorescein isothiocyanate (FITC)-conjugated rabbit polyclonal anti-MOR1 antibody. DAPI (4′,6-diamidino-2-phenylindole) was used to counterstain the nuclei. As expected, fluorescence microscopy results show that GAD67 staining is localized predominately in the neuronal cytoplasm, with approximately 40% of the population staining. The MOR1-FITC stain showed localization within the cytoplasm and plasma membrane as expected, however, there was also significant staining in the nuclear membrane and neuronal nucleus. MOR1-FITC was present in approximately 35% of the neuronal population. In separate experiments, we used double-immunostaining to study the co-expression of MOR1 and GAD67 within the same NAcc neurons. A similar localization pattern was detected with a small subset, approximately 2%, of neurons expressing both labels. There are few published reports of GAD67 and MOR1 co-expression within neurons of the NAcc. Previous studies of MOR expression show the receptor to be localized to the plasma membrane and, to a smaller degree, intracellularly. Here we found the MOR1 staining to be predominantly in the nucleus and nuclear membrane, with minimal expression on the plasma membrane. Further studies are in progress to validate the nuclear expression of MOR in GABAergic NAcc neurons. Results of the study suggest that a subset of mouse NAcc neurons express both MOR1 and GAD67, providing a direct intracellular link between opioid and GABAergic systems in the reward pathway. Preliminary intranuclear localization of MOR suggests a novel signaling pathway that may be important in fully elucidating neurobiological mechanisms underlying behaviors related to reward and addiction.
SNP Identity Searching Across Ethnicities, Kinship, and Admixture
Authors: Helfer Brian S. , Ricke Darrell O. 
Published: March 2019

ABSTRACTAdvances in high throughput sequencing (HTS) enables application of single nucleotide polymorphism (SNP) panels for identification and mixture analysis. Large reference sets of characterized individuals with documented relationships, ethnicities, and admixture are not yet available for characterizing the impacts of different ethnicities, kinships, and admixture on identification and mixture search results for relatives and unrelated individuals. Models for the expected results are presented with comparison results on twoin silicodatasets spanning four ethnicities, extended kinship relationships, and also admixture between the four ethnicities.
Dynamics ofMycobacterium tuberculosisAg85B revealed by sensitive ELISA
Authors: Ernst Joel D. , Cornelius Amber , Bolz Miriam 
Published: March 2019

AbstractSecretion of specific proteins contributes to pathogenesis and immune responses in tuberculosis and other bacterial infections, yet the kinetics of protein secretion and fate of secreted proteins in vivo are poorly understood. We generated new monoclonal antibodies that recognize theM. tuberculosissecreted protein, Ag85B, and used them to establish and characterize a sensitive ELISA to quantitate Ag85B in samples generated in vitro and in vivo. We found that nutritional or culture conditions had little impact on secretion of Ag85B, and that there is considerable variation in Ag85B secretion by distinct strains in theM. tuberculosiscomplex: compared with the commonly-used H37Rv strain (Lineage 4),M. africanum(Lineage 6) secretes less, and two strains from Lineage 2 secrete more Ag85B. We also used the ELISA to determine that the rate of secretion of Ag85B is 10-to 100-fold lower than that of proteins secreted by gram-negative and gram-positive bacteria, respectively. ELISA quantitation of Ag85B in lung homogenates ofM. tuberculosisH37Rv-infected mice revealed that although Ag85B accumulates in the lungs as the bacterial population expands, the amount of Ag85B per bacterium decreases nearly 10,000-fold at later stages of infection, coincident with development of T cell responses and arrest of bacterial population growth. These results indicate that bacterial protein secretion in vivo is dynamic and regulated, and quantitation of secreted bacterial proteins can contribute to understanding pathogenesis and immunity in tuberculosis and other infections.ImportanceBacterial protein secretion contributes to host-pathogen interactions, yet the process and consequences of bacterial protein secretion during infection are poorly understood. We developed a sensitive ELISA to quantitate a protein (termed Ag85B) secreted byM. tuberculosisand used it to find that Ag85B secretion occurs with slower kinetics than for proteins secreted by gram positive and gram negative bacteria, and that accumulation of Ag85B in the lungs is markedly regulated as a function of the bacterial population density. Our results demonstrate that quantitation of bacterial proteins during infection can reveal novel insights into host-pathogen interactions.
C3G (RapGEF1) localizes to the mother centriole and regulates centriole division and primary cilia dynamics
Authors: Nayak Sanjeev Chavan , Radha Vegesna 
Published: March 2019

AbstractC3G (RapGEF1), a negative regulator of β-catenin, plays a role in cell differentiation and is essential for early embryonic development in mice. In this study, we identify C3G as a centrosomal protein that regulates centriole division and primary cilia dynamics. C3G is present at the centrosome in interphase as well as mitotic cells, but is absent at the centrioles in differentiated myotubes. It interacts with, and co-localizes with cenexin in the mother centriole. Stable clone of cells depleted of C3G by CRISPR/Cas9 showed reduction in cenexin protein, and presence of supernumerary centrioles. Over-expression of C3G resulted in inhibition of centrosome division in normal and hydroxyurea treated cells. Proportion of ciliated cells is higher, and cilia length longer in C3G knockout cells. C3G inhibits cilia formation and length dependent on its catalytic activity. Unlike wild type cells, C3G depleted cells inefficiently retracted their cilia upon stimulation to reenter the cell cycle, and proliferated slowly, arresting in G1. We conclude that C3G inhibits centriole division and maintains ciliary homeostasis, properties that may be important for its role in embryonic development.Summary statementWe identify C3G as a centrosomal protein and regulator of centriole number, primary cilia length and resorption. These properties are important for its role in embryogenesis, and suggest that mutations in C3G could cause ciliopathies.