bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of rheumatoid arthritis (RA) and osteoarthritis (OA) based on microarray profiles of human joint fibroblast‐like synoviocytes
Authors: Cai Peian , Jiang Tongmeng , Li Bo , Qin Xiong , Lu Zhenhui , Le Yiguan , Shen Chong , Yang Yifeng , Zheng Li , Zhao Jinmin 
Published: November 2018

The purpose of the present study was to investigate the underlying molecular mechanism of osteoarthritis (OA) and rheumatoid arthritis (RA) based on microarray profiles. Three human joint fibroblast‐like synoviocytes (FLSs) microarray profiles including 26 OA samples, 33 RA samples, and 20 healthy control (HC) samples were downloaded from the GEO database. Differentially expressed genes (DEGs) between OA and HC (DEGsOA) and RA and HC (DEGsRA) were identified. Co‐expressed and specific genes were analysed between DEGsOA and DEGsRA. Gene ontology, KEGG pathway enrichment, PPI network, and GSEA were performed to predict the function of DEGs. Two hundred seventy‐six and 410 differential genes in DEGsOA and DEGsRA were observed. One hundred fifty coexpressed genes and 126 OA‐specific genes (SELE, SERPINE1, and NFKBIA were the key genes) between DEGsOA and DEGsRA were enriched in the tumour necrosis factor (TNF) signalling pathway. However, 260 RA‐specific genes of which the key genes were CCR5, CCR7, CXCR4, CCL5, and CCR4 were enriched in chemokine signalling pathway. Therefore, FLSs might exert an inflammatory effect by regulating TNF signalling pathway, targeting SELE, SERPINE1, and NFKBIA during the process of OA. Although TNF signalling pathway was also involved in the synovitis of RA, chemokine signalling pathway played the key role in RA FLSs mediating cell migration, invasion, and release of chemotaxis. In addition, CCR5, CCR7, CXCR4, CCL5, and CCR4 might be hub genes in RA. The different biomarkers and pathways identified in OA and RA may provide references for further study.Significance of the studyThis study revealed the similar and different mechanisms of FLSs and different biomarkers that might with important regulatory effects on RA and OA. In OA, FLSs played an inflammatory role through TNF signalling pathway, targeting SELE, SERPINE1, and NFKBIA. Although TNF signalling pathway was also involved in the synovitis of RA, chemokine signalling pathway was a crucial pathway in mediating FLSs migration, invasion, and release of chemotaxis. CCR5, CCR7, CXCR4, CCL5, and CCR4 might be keys genes in RA. We expect that our results will bring more comprehensively understanding between RA and OA for researchers.
Impact of prosthesis‐iteration evolution and sizing practice on the incidence of prosthesis–patient mismatch after transcatheter aortic valve replacement
Authors: Abdelghani Mohammad , Allali Abdelhakim , Kaur Jatinderjit , Hemetsberger Rayyan , Mehilli Julinda , Neumann Franz‐Josef , Frerker Christian , Kurz Thomas , El‐Mawardy Mohamed , Richardt Gert , Abdel‐Wahab Mohamed 
Published: November 2018

AbstractObjectivesTo investigate the impact of the introduction of the next generation self‐expanding (SE) and balloon‐expandable (BE) transcatheter heart valves (THVs) on the incidence of prosthesis–patient mismatch (PPM) after transcatheter aortic valve replacement (TAVR).BackgroundPPM is a risk factor for accelerated degeneration of bioprosthetic aortic valves. Data on PPM after TAVR are derived mainly from studies of older generation THVs.MethodsPPM was assessed at 30 days post‐TAVR with the older generation (Medtronic CoreValve, n = 120 and Edwards Sapien XT, n = 121) and the next generation THVs (Medtronic Evolut R/Pro, n = 136 and Edwards Sapien 3, n = 363).ResultsThe incidence of any and severe PPM was 15.1% and 0.0% for the older generation THVs, and 42.8% and 12.1% for the next generation THVs. The incidence of moderate and severe PPM was 23.3% and 3.5% in patients who received an Evolut R/Pro vs. 33.1% and 14.7% in those who received a Sapien 3 (P < 0.001). On multivariable analysis, TAVR with the Sapien 3 THV was not associated with PPM, while left ventricular ejection fraction (0.97 [0.95–0.99], P = 0.002), history of myocardial infarction (2.09 [1.00–4.34], P = 0.049), annulus maximum diameter (0.84 [0.77–0.92], P < 0.001), and THV oversizing (0.90 [0.87–0.94], P < 0.001) were independently associated with PPM. In Sapien 3, the risk of any and severe PPM was higher in those with no oversizing (odds ratio: 3.25 [1.23–8.53], P = 0.017 and 5.79[2.33–14.36], P < 0.001).ConclusionsThe incidence of PPM in contemporary TAVR is significant, especially with the next generation BE THV without adequate oversizing.
Compact third‐order microstrip bandpass filter designed by the distributed‐ to lumped‐element equivalence
Authors: Xu Jun , Xiao Fei , Cao Yu , Zhang Yong , Tang Xiaohong 
Published: November 2018

SummaryIn reality, it is not easy to design microstrip filters for the sake of distributed‐element effect. It is an effective approach to approximate distributed‐element structures through appropriate lumped‐element circuits. In this paper, some basic microstrip structures are discussed, whose equivalent lumped‐element circuits are derived. Then, a novel microstrip filter is obtained by connecting these microstrip structures, according to the similar topology of the third‐order lumped‐element bandpass filter. The equivalent lumped‐element counterpart of the proposed microstrip filter clearly reveals its physical mechanism, ie, how the resonances are created and coupled and how the transmission zeros are created. Furthermore, a set of the design formulas are presented, which can be used to calculate initial structure parameters and then facilitate design process. The proposed microstrip bandpass filter can realize a third‐order elliptic bandpass response with one transmission zero at each side of the passband. In addition, two short‐circuited stubs are added to input/output ports to create the third transmission zero. The filter is featured by good frequency selectivity and out‐of‐band suppression. For demonstration, an actual example was designed, fabricated, and measured.
Optimization and validation of simultaneous analyses of ecgonine, cocaine, and seven metabolites in human urine by gas chromatography–mass spectrometry using a one‐step solid‐phase extraction
Authors: Fernández Nicolás , Cabanillas Laura Marina , Olivera Nancy Mónica , Quiroga Patricia Noemí 
Published: November 2018

AbstractThe presence of ecgonine in urine has been proposed as an appropriate marker of cocaine use. Only a few methods have been published for their determination along with cocaine and the rest of its metabolites. Due to their high polarity and consequent solubility in water, these have low recoveries, which is why it is necessary to increase the sensitivity, by the formation of hydrochloric salts or multiderivatization of the analytes or by performing two solid‐phase extractions (SPEs), considerably increasing the time and cost of the analysis. This work describes a fast and fully validated procedure for the simultaneous detection and quantification of ecgonine, ecgonine‐methyl‐ester, benzoylecgonine, nor‐benzoylecgonine, m‐hydroxybenzoylecgonine, cocaethylene, cocaine, norcocaine, and norcocaethylene in human urine (500 μL) using one SPE and simple derivatization. Separation and quantification were achieved by gas chromatography–electron ionization–mass spectrometry (GC–EI–MS) in selected‐ion monitoring mode. Quantification was performed by the addition of deuterated analogs as internal standards. Calibration curves were linear in the adopted ranges, with determination coefficients higher than 0.99. The lower limits of quantification ranged from 2.5 to 10 ng/mL. The intra‐ and inter‐day precision, calculated in terms of relative standard deviation, were 1.2%–14.9% and 1.8%–17.9%, respectively. The accuracy, in terms of relative error, was within a ± 16.4% interval. Extraction efficiency ranged from 84% to 103%. Compared with existing methods, the procedure described herein is fast, since only one SPE is required, and cost‐effective. In addition, this method provides a high recovery for ecgonine, resulting in a better alternative to the previously published methods.
Expression of PD‐L1 and CTLA‐4 in female urethral carcinoma
Authors: Ninomiya Sahoko , Kawahara Takashi , Mochizuki Taku , Ishiguro Yukari , Miyoshi Yasuhide , Otani Masako , Yao Masahiro , Miyamoto Hiroshi , Uemura Hiroji 
Published: November 2018

IntroductionAlthough the tumors are often easily detected, a considerable number of patients with female urethral carcinoma are diagnosed in an advance stage. Thus, no evidence‐based therapeutic approach has been established. We herein report our experience in the treatment of three female patients with urethral carcinoma. We also examined the expression of PD‐L1 and CTLA‐4.Case presentationThree female patients pathologically diagnosed with urethral carcinoma, including urothelial carcinoma, squamous cell carcinoma, and adenocarcinoma, between 2013 and 2017 were analyzed in this study. Two patients underwent urethrectomy with cystostomy. Immunohistochemistry was performed to assess the levels of PD‐L1 and CTLA‐4 expression in patients with urethral carcinoma. Eleven control cases of urethral carcinoma tissue were also stained.ConclusionThis study revealed the expression of PD‐L1 and CTLA‐4 in female urethral carcinomas.
Evolving Business Intelligence and Data Analytics in Higher Education
Authors: Drake Brent M. , Walz Aaron 
Published: November 2018

AbstractOver the past 20 years, many colleges and universities have made significant progress in building data warehouses and implementing business intelligence tools to provide better access to administrative data that were previously locked away in enterprise systems. However, the data architectures from the 1990s and 2000s are aging as new technologies offer expanded possibilities to collect, manage, and engage with data, including tapping into sources of data that were not available using previous approaches. This is coming at a time where there are increasing demands from stakeholders across higher education (internal and external) to “see the data” in response to constricting sources of funding, pressure to make higher education more affordable, changing student populations, and increased focus on student success and on‐time graduation. This chapter starts with a look at the historical approaches most schools have taken, then explores new analytic opportunities for IR offices and the impact these new technologies have on its operations while providing support for data‐driven decision‐making. The chapter concludes with a look at the future of business intelligence (BI) and data analytics and what it means for higher education.
A practical first step using needs assessment and a survey approach to implementing a clinical pharmacogenomics consult service
Authors: Zakinova Angela , Long‐Boyle Janel R. , French Deborah , Croci Rhiannon , Wilson Leslie , Phillips Kathryn A. , Kroetz Deanna L. , Shin Jaekyu , Tamraz Bani 
Published: November 2018

IntroductionGenetic‐guided selection of nononcologic medications is not commonly practiced in general, and at University of California, San Francisco (UCSF) Health, specifically. Understanding the unique position of clinicians with respect to clinical pharmacogenetics (PG) at a specific institution or practice is fundamental for implementing a successful PG consult service.ObjectivesTo assess clinicians' current practices, needs, and interests with respect to clinical PG at UCSF Health, a large tertiary academic medical center.MethodsA list of 42 target medications with clinical PG recommendations was complied. Clinical specialties that routinely used the target medications were identified. A 12‐question survey focused on practice of PG for target medications was developed. Pharmacists and physicians were surveyed anonymously in several clinical specialties. Survey results were analyzed using descriptive statistics.ResultsOf the 396 clinicians surveyed, 76 physicians and 59 pharmacists participated, resulting in 27% and 50% average response rates, respectively. The current use of PG in clinical practice for physicians and pharmacists was 29% and 32%, respectively, however this number varied across clinical specialties from 0% to 80%. Of clinicians whom reported they do not currently apply PG, 63% of physicians and 54% of pharmacists expressed interest in integrating PG. However, the level of interest varied from 20% to 100% across specialties. Of the respondents, 64% of physicians and 56% of pharmacists elected to provide contact information to investigators to further discuss their interest related to clinical PG.ConclusionsWhile PG is not uniformly practiced at UCSF Health, there is considerable interest in utilizing PG by the respondents. Our approach was successful at identifying clinicians and services interested in PG for specific drug‐gene pairs. This work has set a foundation for next steps to advance PG integration at UCSF Health. Clinicians can adopt our approach as preliminary work to build a clinical PG program at their institutions.
Cancer stem cells as a therapeutic target in bladder cancer
Authors: Aghaalikhani Nazi , Rashtchizadeh Nadereh , Shadpour Pejman , Allameh Abdolamir , Mahmoodi Marzieh 
Published: November 2018

AbstractBladder cancer is one of the most prevalent genitourinary cancers responsible for about 150,000 deaths per year worldwide. Currently, several treatments, such as endoscopic and open surgery, appended by local or systemic immunotherapy, chemotherapy, and radiotherapy are used to treat this malignancy. However, the differences in treatment outcome among patients suffering from bladder cancer are considered as one of the important challenges. In recent years, cancer stem cells, representing a population of undifferentiated cells with stem‐cell like properties, have been eyed as a major culprit for the high recurrence rate in superficial papillary bladder cancer.Cancer stem cells have been reported to be resistant to conventional treatments, such as chemotherapy, radiation, and immunotherapy, which induce selective pressure on tumoral populations resulting in selection and growth of the resistant cells. Therefore, targeting the therapeutic aspects of cancer stem cells in bladder cancer may be promising.In this study, we briefly discuss the biology of bladder cancer and then address the possible relationship between molecular biology of bladder cancer and cancer stem cells. Subsequently, the mechanisms of resistance applied by cancer stem cells against the conventional therapeutic tools, especially chemotherapy, are discussed. Moreover, by emphasizing the biomarkers described for cancer stem cells in bladder cancer, we have provided, described, and proposed targets on cancer stem cells for therapeutic interventions and, finally, reviewed some immunotargeting strategies against bladder cancer stem cells.
Circulating visfatin levels and cancers risk: A systematic review and meta‐analysis
Authors: Mohammadi Masoumeh , Mianabadi Faezeh , Mehrad‐Majd Hassan 
Published: November 2018

AbstractVisfatin levels have been reported to be abnormal in many types of cancers. However, epidemiological studies yielded inconsistent results. Therefore, a meta‐analysis was performed to assess the association between circulating visfatin levels and cancer risk. A systematic search was conducted for relevant studies in health‐related electronic databases up to March 2018. Data related to standard mean difference (SMD) and overall odds ratio (ORS) were collected and analyzed. Summary SMD and pooled OR with 95% CIs were calculated using a random‐effect model. Funnel plot and Egger's linear regression test were conducted to examine the risk of publication bias. A total of 27 studies with 2,693 cases and 3,040 healthy controls were included in meta‐analysis for pooling SMD analysis. The results of the meta‐analysis showed a significant higher visfatin levels in patients with various cancers than in controls, with a pooled SMD of 0.88, 95% CI = 0.56–1.20, p = 0.000. In subgroup, metaregression, Galbraith plot, and sensitivity analysis showed no substantial difference among all the analyzed factors. Data from 14 studies were also used for pooling ORs analysis. Metaresults revealed that high visfatin levels were associated with cancer risk (OR = 1.24, 95% CI: 1.14–1.34, p = 0.000). No evidence of publication bias was observed for pooling ORs and SMD analysis. This meta‐analysis indicated a significant association between high circulating visfatin levels and increased risk of various cancers. Visfatin may represent a potential biomarker for early detection of cancers who may benefit from preventive treatment.Note.
An overview of microRNAs: Biology, functions, therapeutics, and analysis methods
Authors: Saliminejad Kioomars , Khorram Khorshid Hamid Reza , Soleymani Fard Shahrzad , Ghaffari Seyed Hamidollah 
Published: November 2018

AbstractMicroRNAs (miRNAs) are a class of small noncoding RNAs, which function in posttranscriptional regulation of gene expression. They are powerful regulators of various cellular activities including cell growth, differentiation, development, and apoptosis. They have been linked to many diseases, and currently miRNA‐mediated clinical trial has shown promising results for treatment of cancer and viral infection. This review provides an overview and update on miRNAs biogenesis, regulation of miRNAs expression, their biological functions, and role of miRNAs in epigenetics and cell–cell communication. In addition, alteration of miRNAs following exercise, their association with diseases, and therapeutic potential will be explained. Finally, miRNA bioinformatics tools and conventional methods for miRNA detection and quantification will be discussed.
KCNQ1OT1 facilitates progression of non‐small‐cell lung carcinoma via modulating miRNA‐27b‐3p/HSP90AA1 axis
Authors: Dong Zhiwu , Yang Ping , Qiu Xiaojian , Liang Shuang , Guan Bing , Yang Haisheng , Li Feifei , Sun Li , Liu Huiling , Zou Guanghui , Zhao Kewen 
Published: November 2018

AbstractLong noncoding RNA KCNQ1OT1 participates in the regulation of imprinted genes within the kcnq1 domain. But its roles in carcinogenesis and metastasis remain largely elusive. Herein, we evaluated its potential in non‐small‐cell lung cancer (NSCLC) progression. We demonstrated that the KCNQ1OT1 level was upregulated in NSCLC tissues and cell lines. High KCNQ1OT1 level correlated with poor overall and progression‐free survival in NSCLC patients. KCNQ1OT1 facilitated proliferation, migration, and invasion in H460 cells. Furthermore, knockdown of KCNQ1OT1 reduced the expression of HSP90AA1. KCNQ1OT1 presented a positive correlation with HSP90AA1 which predicted the tumor progression in NSCLC from The Cancer Genome Atlas database. Intriguingly, KCNQ1OT1 modulated HSP90AA1 expression by sponging miR‐27b‐3p. MiR‐27b‐3p counteracted the effect of KCNQ1OT1 on HSP90AA1 expression, H460 cell migration, and invasion. These data revealed a role for KCNQ1OT1 as an oncogene through miR‐27b‐3p/HSP90AA1 axis during NSCLC progression.
Retracted: Synthesis, Characterization, and Surface Activities of Polymeric Cationic Thiol Surfactants in Aqueous Medium
Authors: Zaky Mohamed F. , Sabbah Ibrahim A. , Hendawy Mostafa E. , Abdel Hameed Reda S. , Negm Nabel A. 
Published: November 2018

AbstractA series of cationic polyurethane surfactants [PQ8‐18] were synthesized by the reaction of alkyl bromoacetate (namely: octyl‐, decyl‐, dodecyl‐, tetradecyl‐, hexadecyl‐, and octadecyl bromoacetate) as quaternizing agents and modified polyurethane contains tertiary amine species. Modified polyurethane was prepared by the reaction of toluene diisocyanate (TDI) and triethanol amine monomercaptoacetate. The chemical structures of the prepared surfactants were confirmed using elemental analysis, Fourier transform infrared spectroscopy (FTIR), and Proton nuclear magnetic resonance (1H NMR) spectroscopy. The molecular weight measurements of the prepared polymers showed that the segments of each polymer contain average 10 units of the urethane‐triethanol amine mercaptoacetate. The surface activities of the prepared surfactants including: surface tension (γ), effectiveness (
πcmc), concentration at micelle formation (CMC), efficiency (Pc20), maximum concentration at the interface (Γmax), and the average area occupied by each surfactant molecule at the interface at equilibrium (
A
min) of surfactants solutions were established at 25°C. The surface tension and the critical micelle concentration values of the prepared surfactants were gradually decreased by the gradual increase of their alkyl chain length. The prepared cationic surfactants showed efficient activity as inhibitors for dissolution of carbon steel in an acidic medium and also as a biocide against the growth of bacteria, fungi, and yeast.
Mechanical properties and quality parameters of chitosan‐edible algae (Palmaria palmata) on ready‐to‐eat strawberries
Authors: Rico Daniel , Barcenilla Beatriz , Meabe Agate , González Cristina , Martín‐Diana Ana B 
Published: November 2018

AbstractBACKGROUNDStrawberries are appreciated for their taste, flavor, and juiciness; however, they are highly perishable during postharvest, handling, and storage stages, producing significant physical damage and loss of vitamins and other phytonutrients. This study therefore aimed to determine the applicability of edible coatings as an environmentally friendly strategy to improve the fresh appearance of strawberries and extend their shelf life.RESULTSThe effectiveness of edible coatings formulated with chitosan (CHC) or chitosan and algae (Palmaria palmata Kuntze) (CH‐PC) was investigated with regard to quality and nutritional parameters for ready‐to‐eat strawberries processed and stored at 4 °C for 10 days. Chitosan significantly (P < 0.05) controlled the gradual decline of strawberries, reducing their respiration rate, microbial load, and nutritional loss. The addition of P. palmata to the edible coating significantly (P < 0.05) enhanced anthocyanin and ascorbic acid content.CONCLUSIONThese results suggest that CH‐PC could be used in the strawberry postharvest industry to slow senescence, while reducing product loss and enhancing its nutritional value. © 2018 Society of Chemical Industry
Quantitative risk assessment of Listeria monocytogenes in bulk cooked meat from production to consumption in China: a Bayesian approach
Authors: Sun Wanxia , Liu Yangtai , Wang Xiang , Liu Qing , Dong Qingli 
Published: November 2018

AbstractBACKGROUNDTo estimate the public health risk related to cooked meat in bulk products contaminated with Listeria monocytogenes, a generic Bayesian network (BN) risk‐assessment model was developed to simulate influencing factors and processes of products from the industry level to the consumer level. To quantify the model, parameter values of prior distributions were acquired from the literature, websites, and expert opinions. Using the Markov chain Monte Carlo (MCMC) simulation approach, posterior probability distributions were calculated according to the incorporated evidence, which allowed us to predict various risks affected by processing variability from production to consumption.RESULTSThe average risks of listeriosis from consuming cooked meat in bulk products are 8.40 × 10−7, 2.58 × 10−8, 8.24 × 10−7, and 1.05 × 10−6 per meal for children, young people, elderly people, and pregnant women, respectively. The estimated mean number of listeriosis cases is 5 per 100 000 people per year in China.CONCLUSIONAlthough only a conceptual BN model is given, it manifests the principles and characteristics of mathematical methods. The BN model can also provide significant benefits for quantitative risk assessment by incorporating all available data and by updating beliefs. © 2018 Society of Chemical Industry