bluuebunny/crossref_metadata_2025_split huggingface dataset:
A variant in CYP2R1 predicts circulating vitamin D levels after supplementation with high‐dose of vitamin D in healthy adolescent girls
Authors: Khayyatzadeh Sayyed Saeid , Mehramiz Mehrane , Esmaeily Habibollah , Mirmousavi Seyed Jamal , Khajavi Leila , Salehkhani Fatemeh Nejati , Hanachi Parichehr , Bahrami‐Taghanaki Hamidreza , Eslami Saeed , Vatanparast Hasan , Ferns Gordon A. , Avan Amir , Ghayour‐Mobarhan Majid 
Published: January 2019

AbstractAimThe determinants of serum vitamin D seems to be the environmental factors (dietary and supplementary intake and exposure to ultraviolet light) and genetic factors. We aimed to study the relationship between a vitamin D‐associated genetic polymorphism and serum 25(OH)D concentrations in healthy adolescent girls in Iran, and its effects on a high‐dose supplement of vitamin D.Material and methodA total of 616 healthy adolescent girls with mean age 15 received 50,000 IU of vitamin D3 weekly over 9 weeks. Serum vitamin D levels and other metabolic factors were measured at baseline and after the intervention. The genotyping of the CYP2R1 variant (rs10741657) was performed by TaqMan genotyping assays.ResultsRegardless of the genetic background, at baseline, 87% of adolescent girls were vitamin D deficient (serum 25(OH)D level < 50 nmol/l). High‐dose supplementation with VitD reduced the proportion of girls who were deficient substantially to about 24%. The genetic analysis revealed that although at baseline there was not a gene‐vitamin D association (
p trend = 0.1), the response to supplementation appeared to be modulated by this variant (
p trend < 0.001). However, other anthropometric and biochemical measures were not affected by this intervention, over this short period. Serum 25(OH)D was increased in all participants although the carriers of the minor A allele seemed to be better responders so that the percentages of the change serum vitamin D in the holder of AA and AG genotypes were 539.4 ± 443.1 and 443.7 ± 384.6, respectively, compared with those with common GG genotype (363.3 ± 354.0). Our regression analysis revealed that the probability of an increase in serum 25(OH)D in a participant with AA genotype was 2.5‐fold greater than those with a GG genotype (OR = 2.5 (1.4–4.4);
p value = 0.002).ConclusionBased on our findings, it appears that the rs10741657 variant of the CYP2R1 gene modulates the response to high‐dose of vitamin D supplementation.
Increased autocrine interleukin‐6 production is significantly associated with worse clinical outcome in patients with chronic lymphocytic leukemia
Authors: Wang Hua‐Qing , Jia Li , Li Yu‐Ting , Farren Timothy , Agrawal Samir G. , Liu Feng‐Ting 
Published: January 2019

AbstractChronic lymphocytic leukemia (CLL) remains incurable with current standard therapy. We have previously reported that an increased expression of interleukin‐6 (IL‐6) receptor CD126 leads to resistance of CLL cells to chemotherapy and worse prognosis for patients with CLL. In this study, we determine whether autocrine IL‐6 production by CLL B cells is associated with poor clinical outcome and explore IL‐6‐mediated survival mechanism in primary CLL cells. Our results demonstrate that higher levels of autocrine IL‐6 are significantly associated with shorter absolute lymphocyte doubling time, patients received treatment, without complete remission, advanced Binet stages, 17p/11q deletion, and shorter time to first time treatment and progression‐free survival. IL‐6 activated both STAT3 and nuclear factor kappa B (NF‐κB) in primary CLL cells. Blocking IL‐6 receptor and JAK2 inhibited IL‐6‐mediated activation of STAT3 and NF‐κB. Our study demonstrates that an increased autocrine IL‐6 production by CLL B‐cells are associated with worse clinical outcome for patients with CLL. IL‐6 promotes CLL cell survival by activating both STAT3 and NF‐κB through diverse signaling cascades. Neutralizing IL‐6 or blocking IL‐6 receptor might contribute overcoming the resistance of CLL cells to chemotherapy. We propose that the measurement of autocrine IL‐6 could be a useful approach to predict clinical outcome.
Four lncRNAs associated with breast cancer prognosis identified by coexpression network analysis
Authors: Li Jie , Gao Chundi , Liu Cun , Zhou Chao , Ma Xiaoran , Li Huayao , Li Jia , Wang Xue , Qi Lingyu , Yao Yan , Zhang Xiaoming , Zhuang Jing , Liu Lijuan , Wang Kejia , Sun Changgang 
Published: January 2019

AbstractPrevious studies on long noncoding RNA (lncRNA) have made breakthroughs in the treatment of several tumors, and these findings have brought attention to the lncRNA signature of breast cancer. Increased understanding of genomic architecture and achievement of innovative therapeutic strategies has prompted creation of a novel oncological model for the treatment of solid cancers. In this study, we systematically analyzed the transcriptome of breast cancer tissues to gain more in‐depth knowledge of tumor biology. Gene coexpression relationships were studied in 206 samples from The Cancer Genome Atlas database, and nine coexpression modules were identified. After screening and analysis, we identified four important prognosis‐related lncRNAs (HOTAIR, SNHG16, HCP5, and TINCR), and constructed a prognostic model, one (HCP5) of which has not previously been identified in the context of breast cancer. Importantly, an understanding of prognosis facilitates precise disease risk assessment and advances the selection of strategies for risk‐adaptive management. These findings broaden the landscape of carcinogenic lncRNAs in breast cancer, providing insights into the biological significance and clinical application of lncRNAs in breast cancer.
Dexmedetomidine improves acute stress‐induced liver injury in rats by regulating MKP‐1, inhibiting NF‐κB pathway and cell apoptosis
Authors: Sha Jichen , Feng Xiujing , Chen Yongping , Zhang Huayun , Li Bei , Hu Xueyuan , Fan Honggang 
Published: January 2019

AbstractAcute stress is a frequent and unpredictable disease for many animals. Stress is widely considered to affect liver function. However, the underlying mechanism by which dexmedetomidine (DEX) attenuates acute stress‐induced liver injury in rats remains unclear. In this study, we used forced swimming for 15 min and acute 3‐hr restraint stress model. Behavioral tests and changes in norepinephrine levels confirmed the successful establishment of the acute stress model. Acute stress‐induced liver injury, evidenced by hematoxylin and eosin‐stained pathological sections and increased serum aminotransferase and aspartate aminotransferase levels, was reduced in DEX‐treated livers. Reactive oxygen species and oxidative stress levels were dramatically decreased with DEX treatment compared with acute stress‐induced liver injury. DEX significantly reduced acute stress‐induced liver inflammation and apoptosis, as assessed by terminal deoxynucleotidyl transferase dUTP nick‐end labeling staining and inflammation and apoptosis‐related protein levels. DEX treatment also effectively inhibited acute stress‐induced c‐Jun N‐terminal kinase (JNK), P38, and BAD signaling pathway activation, and significantly induced MKP‐1 activation. Thus, DEX has a protective effect on acute‐stress‐induced liver injury by reducing inflammation and apoptosis, which suggests a potential clinical application for DEX in stress syndrome.
Aerobic exercise can modulate the underlying mechanisms involved in the development of diabetic complications
Authors: Yaribeygi Habib , Butler Alexandra E. , Sahebkar Amirhossein 
Published: January 2019

AbstractDiabetes mellitus is a highly prevalent metabolic disorder that affects many molecular pathways, causing a shift from a physiologic to a pathophysiologic state. Alterations in the molecular pathways promote diabetic complications and, thus, many medical and nonmedical therapies have been directed at preventing these complications. Despite the beneficial effects on moderating glycemic control, medical therapies may also have unfavorable side effects. This makes nonmedical therapeutic approaches more attractive due to lower pharmacological side effects of these strategies compared to medical agents. Aerobic exercise is now considered as a major nonmedical strategy that can promote beneficial and protective effects to counteract the development of diabetic complications via attenuation of the major molecular mechanisms involved in diabetes.
Promotional effect of short‐chain saturated alcohols on Fe3O4‐catalyzed decomposition of H2O2 and its application in selective oxidation of benzyl alcohol
Authors: Zhang Jiawei , Xiao Shiwei , Chen Rong , Chen Fengxi 
Published: January 2019

AbstractBACKGROUNDHydrogen peroxide (H2O2) has been used widely in diverse areas of biotechnology, environmental remediation and organic synthesis wherein its catalytic activation to generate reactive oxygen species (e.g. hydroxyl radicals) is of vital importance. Magnetite (Fe3O4) microspheres are attractive heterogeneous peroxidase‐like catalysts due to many technological advantages (e.g. biocompatible, stable and magnetically recoverable). However, its peroxidase‐like activity has to be improved for practical applications.RESULTSSmall amounts of short‐chain saturated alcohols (e.g. methanol, ethanol, iso‐propanol, tert‐butanol, mono‐ethylene glycol and glycerol) increased collision frequency between H2O2 and Fe3O4 (e.g. by about two orders of magnitude with the addition of 1 mol% glycerol) via facilitating the nucleophilic addition of H2O2 to surface Fe sites and promoted Fe3O4‐catalyzed decomposition of H2O2 and selective oxidation of benzyl alcohol with H2O2 in water. In addition, Fe3O4 microspheres were magnetically recoverable and reusable for at least five times without loss of catalytic activity and selectivity.CONCLUSIONSThe Fe3O4/trace alcohol catalytic system decomposed H2O2 effectively. Its potential application was demonstrated by selective oxidation of benzyl alcohol with H2O2 in water in this work. This simple and efficient catalytic system may be applied also to other oxidative transformations in organic syntheses, or to oxidative degradation of organic pollutants in environmental remediation. © 2019 Society of Chemical Industry
Sepsis erodes CD8+ memory T cell-protective immunity against an EBV homolog in a 2B4-dependent manner
Authors: Xie Jianfeng , Crepeau Rebecca L , Chen Ching-wen , Zhang Wenxiao , Otani Shunsuke , Coopersmith Craig M , Ford Mandy L 
Published: January 2019

Abstract
Epstein–Barr virus (EBV) reactivation commonly occurs following sepsis, but the mechanisms underlying this are unknown. We utilized a murine EBV homolog (gHV) and the cecal ligation and puncture model of polymicrobial sepsis to study the impact of sepsis on gHV reactivation and CD8+ T cell immune surveillance following a septic insult. We observed a significant increase in the frequency of gHV-infected germinal center B cells on day 7 following sepsis. This increase in viral load was associated with a concomitant significant decrease in the frequencies of gHV-specific CD8+ T cells, as measured by class I MHC tetramers corresponding to the immunodominant viral epitopes. Phenotypic analysis revealed an increased frequency of gHV-specific CD8+ T cells expressing the 2B4 coinhibitory receptor in septic animals compared with sham controls. We sought to interrogate the role of 2B4 in modulating the gHV-specific CD8+ T cell response during sepsis. Results indicated that in the absence of 2B4, gHV-specific CD8+ T cell populations were maintained during sepsis, and gHV viral load was unchanged in 2B4−/− septic animals relative to 2B4−/− sham controls. WT CD8+ T cells upregulated PD-1 during sepsis, whereas 2B4−/− CD8+ T cells did not. Finally, adoptive transfer studies revealed a T cell-intrinsic effect of 2B4 coinhibition on virus-specific CD8+ T cells and gHV viral load during sepsis. These data demonstrate that sepsis-induced immune dysregulation erodes antigen-specific CD8+ responses against a latent viral infection and suggest that blockade of 2B4 may better maintain protective immunity against EBV in the context of sepsis.
Cisplatin interacting with buffering media and cysteine: Molecular insight due to Raman microspectroscopy
Authors: Šišková Karolína M. 
Published: January 2019

AbstractThe solvation of cisplatin, [Pt(NH3)2Cl2], a widely used chemotherapeutical drug, regarded on molecular level in model media is often overlooked by researchers. It is, however, essential in several aspects. The solvation was monitored by using Raman scattering microspectroscopy. This technique being used for the first time enables us to focus the laser beam several micrometers above the dissolving crystals of cisplatin, allowing to monitor the process of cisplatin dissolution in situ. The obtained Raman microspectroscopic results provided a direct qualitative evidence about (a) interaction between Tris, MOPS, and HEPES buffers and cisplatin via PtN coordination covalent bond and (b) interaction of cisplatin with cysteine dominantly through PtS bond (at pH 7.4). Furthermore, this vibrational spectroscopic technique revealed a decreased dissolution of cisplatin in a particular buffering solution in the presence of NaCl abundance; kinetics of the PtN formation in the case of MOPS–NaCl medium was monitored. Moreover, spatial dissolution of cisplatin in Cys‐Tris medium was observed in situ. The particular buffering medium, containing either primary or tertiary amines, can interact with cisplatin via a coordination covalent bond. The presence of an increased NaCl concentration can partially suppress this interaction. Raman microspectroscopy, a method of vibrational spectroscopy, is a powerful technique to investigate the dissolution of cisplatin in situ. On the basis of vibrational microspectroscopic measurements, it provides direct evidence about cisplatin interactions with selected model media being widely used in biochemistry and biophysics. This interaction could influence the effectiveness of cisplatin as a drug.
Fortification and bioavailability of zinc in potato
Authors: Vergara Carmona Victor M , Cecílio Filho Arthur B , Almeida Hilário J de , Gratão Priscila Lupino 
Published: January 2019

AbstractBACKGROUNDMost agricultural soils have low zinc (Zn) content available to crops, which results in a significant decrease in productivity and in public health problems. However, the priming of potato tubers in solutions with Zn can be an effective strategy for their fortification. In order to evaluate the effect of Zn concentrations and tuber priming time on the fortification and bioavailability of Zn, potato tubers were primed in solutions containing 0, 10, 20 and 30 mg mL−1 Zn during 12, 16, 20, and 24 h, respectively. The dry matter and the content of Zn and phytic acid (PA) in tubers were assessed in order to obtain the PA:Zn molar ratio.RESULTSLonger priming time increased the Zn content in the cortex of the tubers. High Zn concentration in the solution increased the content of Zn linearly in both the cortex and the central region of the tuber, whereas in the periderm the content levels adjusted to the non‐linear logistical model, showing saturation at a minimum of 10 mg mL−1 Zn in the solution. An increase in the bioavailability of Zn was verified when there was higher Zn concentration in the solution.CONCLUSIONA substantial increase in Zn bioavailability was obtained by priming the tubers for 12 h in 10 mg mL−1 Zn. © 2019 Society of Chemical Industry
Comparative analysis of nucleosides, nucleobases, and amino acids in different parts of Angelicae Sinensis Radix by ultra high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry
Authors: Qu Cheng , Yan Hui , Zhu Shao‐Qing , Qian Yi‐Yun , Zhou Gui‐Sheng , Guo Sheng , Zhu Zhen‐Hua , Zhang Sen , Qian Da‐Wei , Duan Jin‐Ao 
Published: January 2019

AbstractA rapid and sensitive ultra high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry method was established and employed to determine 21 nucleosides, nucleobases, and amino acids in 60 samples from different parts of Angelicae Sinensis Radix. The established methods were validated by good linearity (r2 > 0.9937), limits of detection (0.12–77.75 ng/mL), limits of quantitation (0.31–272.13 ng/mL), intra‐ and interday precisions (RSD ≤ 4.84%, RSD ≤ 6.26%), stability (RSD ≤ 5.92%), repeatability (RSD ≤ 7.14%), recovery (91.4–103.4%), and matrix effects (0.92–1.03). Chemical comparative analysis revealed that the content of total analytes in four parts of Angelicae Sinensis Radix were different, and exhibited the order: Head (14.89 mg/g) > Body (10.15 mg/g) > All (8.22 mg/g) > Tail (6.23 mg/g). Principal component analysis showed that the samples could be classified into four groups in accord with four different parts of Angelicae Sinensis Radix. The results could provide a scientific basis and reference for the quality control of Angelicae Sinensis Radix, and may be conducive to further research on the pharmacological activities of Angelicae Sinensis Radix.
Incidental thyroid papillary microcarcinoma: survival and follow‐up
Authors: Gorostis Sophie , Raguin Thibaut , Schneegans Olivier , Takeda Catherine , Debry Christian , Dupret‐Bories Agnès 
Published: January 2019

Objectives/HypothesisThe aim was to study the survival of incidental thyroid papillary microcarcinoma patients treated with surgery for benign thyroid disease to validate absence of oncological follow‐up and reduce unnecessary health expenses.Study DesignRetrospective cohort study.MethodsWe analyzed patient's files and interviewed 252 patients by telephone whose cases were submitted to the multidisciplinary meeting of thyroid pathology in Strasbourg, France, for incidental thyroid papillary microcarcinoma without clinical lymph node involvement, between January 1996 and December 2012.ResultsThirteen patients (5.8%) died while the data were being collected; however, none of the deaths were due to the thyroid pathology, and no patients showed signs of relapse of the thyroidectomy or cervical lymph node level.ConclusionsOur retrospective study shows that patients with incidental localized thyroid papillary microcarcinoma who underwent surgery without radioactive iodine treatment have an identical survival compared to the general population at the same age to validate absence of oncological follow‐up and reduce unnecessary health expenses.Level of Evidence3Laryngoscope, 129:1722–1726, 2019
Recovery of olfaction after sinus surgery for chronic rhinosinusitis: A review
Authors: Haxel Boris R. 
Published: January 2019

ObjectiveInvestigation of the postoperative olfactory function after sinus surgery for chronic rhinosinusitis (CRS) with and without polyps in a review.Data SourcesPubMed.Review MethodsA comprehensive literature search was conducted through June 2018 using relevant keywords. The titles/abstracts were reviewed to identify studies evaluating the sense of smell in CRS‐patients pre‐ and postoperatively by either using the whole or parts of the University of Pennsylvania Smell Identification Test or the Sniffin’ Sticks test. Study characteristics and outcome data of the included studies were extracted.ResultsIn total, 106 studies were retrieved. Twenty‐four studies comprising 1,956 patients were included after quality assessment. The number of patients investigated per study was 19 to 206. In the 24 studies, 959 patients with nasal polyps and 516 without nasal polyps were described. The follow‐up times varied between 0.5 and 28 months. Twenty‐three studies reported an improved sense of smell, at least in certain subgroups. An overall improvement was seen in approximately 50% of the included patients. Nasal polyposis and preoperative anosmia were associated with a higher chance of improvement in olfaction. In those studies that commented on deterioration after sinus surgery, a decrease in olfaction was found in a range from 0% to 10% of cases.ConclusionOlfaction can be improved by sinus surgery in about every second patient, especially if the patient had chronic rhinosinusitis with polyps, was anosmic, and had no prior surgery. A deterioration of sense of smell after surgery is rare. Laryngoscope, 129:1053–1059, 2019
Silsesquioxane‐Containing Hybrid Nanomaterials: Fascinating Platforms for Advanced Applications
Authors: Dong Fuping , Lu Liangyu , Ha Chang‐Sik 
Published: January 2019

AbstractSilsesquioxanes (SSQs), with the general formula, (RSiO1.5)n—where R stands for an organic group, such as alkyl, aryl, alkoxy, or H—are a type of molecular‐level organic/inorganic hybrid silica‐based material. These materials contain reactive or nonreactive organic moieties as well as inorganic Si–O–Si frameworks. In the past few years, extensive efforts have been made using SSQs to construct multifunctional nanocomposites with suitable properties for a range of applications. In this review, the recent various applications of SSQ‐containing hybrid materials are discussed, in addition to updates of the nanocomposite applications. Various physical structures and chemical reactions in SSQ‐based hybrid nanomaterials are emphasized with regard to applications in the field of polymer nanocomposites, catalysts, adsorption, sensors, and biomedicine. This review focuses on results reported in the recent five years (2013–2018).
Feeding Regime Has Little Impact on Weight Loss of Hybrid Crappies Pomoxis spp. and Redear Sunfish at a Low (Winter) Water Temperature when Fed a Noncommercial Diet of Blood Worms
Authors: Roy Luke A , Rawles Steven D , Kelly Anita M , Stone Nathan , Haukenes Alf , Webster Carl D 
Published: January 2019

Abstract
Winter mortality is a problem for fish producers in temperate regions of the USA, including Arkansas. Hybrid crappies (Black Crappie Pomoxis nigromaculatus × White Crappie P. annularis) and Redear Sunfish Lepomis microlophus are popular among recreational anglers. Methods of winter feeding vary widely among existing commercial farms. The impact of different winter feeding regimes on performance of hybrid crappies and Redear Sunfish was evaluated in temperature-controlled aquarium systems. Juvenile hybrid crappies and Redear Sunfish were cultured at approximately 9°C to mimic winter conditions. Three feeding regimes were implemented (3 tanks/regime) in which an ad libitum ration of blood worms was administered twice per week, once per week, or once per month. After 13 weeks of culture, there were no observed differences in weight loss, survival, specific growth rate, or final individual weight for either hybrid crappies or Redear Sunfish. Hybrid crappies lost 27–29% of their initial body weight, while Redear Sunfish lost more than 30% of their initial body weight. Fatty acid composition of both species differed significantly among feeding treatments, showing a decline in saturated fatty acids from initial levels and increases in or static levels of highly unsaturated fatty acids. Results suggest that feeding regime has little effect on weight gain in hybrid crappies and Redear Sunfish when fed at a colder water temperature.