bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Ruellia tuberosa L. on aorta endothelial damage‐associated factors in high‐fat diet and streptozotocin‐induced type 2 diabetic rats
Authors: Ko Chih‐Yuan , Lin Ru‐Hai , Lo Yangming Martin , Chang Wen‐Chang , Huang Da‐Wei , Wu James Swi‐Bea , Chang Yu‐Fang , Huang Wen‐Chung , Shen Szu‐Chuan 
Published: October 2019

AbstractHyperglycemia plays crucial roles in vascular disease development, including macrovascular and microvascular diseases from diabetes mellitus (DM). Our previous study demonstrated that Ruellia tuberosa L. (RTL) aqueous and ethanol extracts alleviate hyperglycemia and inhibit insulin resistance in diabetic rats. This study investigated the protective effect of RTL ethanol extract against aorta dysfunction in high‐fat diet (HFD) and streptozotocin (STZ)‐induced type 2 DM (T2DM) rats. Results showed that RTL ethanol extract (100 and 400 mg/kg BW/day) ameliorated serum lipid profiles, including triglyceride, free fatty acid, low‐density lipoprotein cholesterol, very low‐density lipoprotein cholesterol, and high‐density lipoprotein cholesterol levels. It also significantly reduced the level of serum cytokines such as tumor necrosis factor‐α (TNF‐α) and interleukin‐6 in T2DM rats. Additionally, RTL extract decreased endothelin‐1 and endothelial nitric oxide contents, reduced the level of cell adhesion factors, including monocyte chemoattractant protein‐1 and cell adhesion factor vascular cell adhesion molecule‐1, while decreasing content of damage factors, namely tissue factor and von Willebrand factor in aortic tissues of diabetic rats. Equally noteworthy is that RTL extract enhanced the activity of aorta antioxidative enzymes, including superoxidase dismutase and catalase in diabetic rats, suggesting that RTL ethanol extract may ameliorate aorta dysfunction via enhancing aortic antioxidative enzyme activity, which subsequently suppresses aorta endothelial damage‐associated factors in HFD with STZ‐induced T2DM rats.
Additive Manufactured Graphene Coating with Synergistic Photothermal and Superhydrophobic Effects for Bactericidal Applications
Authors: Jiang Nan , Wang Yilin , Chan Kang Cheung , Chan Ching‐Yuen , Sun Hongzhe , Li Guijun 
Published: October 2019

AbstractDrug‐resistant bacterial infection is a global threat to public health due to the high mobility of the population. Novel therapy methods have been intensively studied for the eradication of antibiotic‐resistant bacteria, including photothermal treatment, which has established outstanding bacterial killing efficiencies under laser radiation, and superhydrophobic surfaces have exhibited excellent antifouling properties. However, an effective, scalable, and affordable bactericidal coating for eliminating drug‐resistant bacteria is lacking. Herein, a novel graphene coating using one‐step laser‐induced graphene and simultaneous laser‐induced forward transfer is introduced. The graphene coating shows high photothermal conversion and superhydrophobic performance, and these synergistic effects can make the bacteria number decrease with over 99.99% proportions under one sun illumination. The superhydrophobic properties can also reduce 99.87% of bacteria compared to the control sample when the solar energy is not available. This additive and scalable method can quickly coat functional graphene onto various substrates, with bacterial applications in many areas, such as water pipeline robots.
Prognose der Festigkeitsentwicklung zementbehandelter Böden mit dem Porosity/Binder‐Index
Authors: Henzinger Christoph , Schömig Philipp 
Published: October 2019

AbstractBöden mit schlechten bautechnischen Eigenschaften können durch Maßnahmen zur Bodenbehandlung für den Erdbau nutzbar gemacht werden. Einer solchen Maßnahme vorauszugehen hat der Nachweis über die Eignung des gewählten Verfahrens. Bei der Durchführung der Eignungsprüfung im Sinne des straßenbautechnischen Regelwerks wird i. d. R. nur die zugegebene Bindemittelmenge variiert. Die Auswertung der Daten erlaubt dann unglücklicherweise keine Verallgemeinerungen über die tatsächlich untersuchten Mischungen hinaus. Dabei ist bekannt, dass neben der Bindemittelmenge auch die Trockendichte die Festigkeit von verbesserten Böden maßgeblich beeinflusst. In der vorliegenden Studie wird über die Anwendbarkeit eines Verfahrens zur realitätsnahen Abbildung des Zusammenhangs zwischen Druckfestigkeit, Trockendichte (Porenanteil) und Bindemittelanteil berichtet und die Anwendbarkeit auf die im Erdbau üblichen, nicht ideal homogenen Mischungen geprüft. Die Analyse stützt sich auf die einaxiale Druckfestigkeit von Probekörpern, die aus Mischungen aus jeweils drei feinkörnigen Böden mit Zement hergestellt wurden. Es wird gezeigt, dass das Verfahren grundsätzlich angewendet werden kann und welche Randbedingungen beachtet werden sollten. Abschließend werden Empfehlungen für die Anwendung des Verfahrens bei der Eignungsprüfung vorgestellt.
Postoperative radiation therapy vs observation for pN1 oral cavity squamous cell carcinoma
Authors: Suresh Krish , Cramer John D. 
Published: October 2019

AbstractBackgroundAmerican Society of Clinical Oncology guidelines recommend that the decision to give postoperative radiotherapy (PORT) for pN1 oral cavity squamous cell carcinoma (OCSCC) without other adverse features be based on the adequacy of the neck dissection (<18 or ≥18 nodes).MethodsWe conducted a cohort study of the National Cancer Database examining how PORT affects survival. We stratified analyses by the adequacy of the neck dissection and lymph node (LN) size.ResultsOur cohort comprised 1909 patients (898 received PORT). PORT conferred a survival benefit in the overall cohort (adjusted hazard ratio 0.82, 95% CI 0.72‐0.94). There was similar benefit in patients receiving inadequate and adequate neck dissections. Patients with >10 mm LN metastasis derived greater benefit compared with patients with smaller metastases.ConclusionsIn pN1 OCSCC without other adverse features, the size of the LN metastases may predict benefit from PORT, whereas the adequacy of the neck dissection may not.
Determination of inclusion depth in ex vivo animal tissues using surface enhanced deep Raman spectroscopy
Authors: Mosca Sara , Dey Priyanka , Tabish Tanveer A. , Palombo Francesca , Stone Nicholas , Matousek Pavel 
Published: October 2019

AbstractThis work presents recent developments in spatially offset and transmission Raman spectroscopy for noninvasive detection and depth prediction of a single SERS inclusion located deep inside ex vivo biological tissues. The concept exploits the differential attenuation of Raman bands brought about by their different absorption due to tissue constituents enabling to predict the inclusion depth. Four different calibration models are tested and evaluated to predict the depth of surface enhanced Raman scattering labelled nanoparticles, within an up to 40 mm slab of porcine tissue. An external measurement carried out in transmission mode, with a noninvasively built model on the analysed sample, is shown to be insensitive to variations of the overall thickness of the tissue yielding an average root‐mean‐square error of prediction of 6.7%. The results pave the way for future noninvasive deep Raman spectroscopy in vivo enabling to localise cancer biomarkers for an early diagnosis of multiple diseases.
Directing hMSCs fate through geometrical cues and mimetics peptides
Authors: Padiolleau Laurence , Chanseau Christel , Durrieu Stéphanie , Ayela Cédric , Laroche Gaétan , Durrieu Marie‐Christine 
Published: October 2019

AbstractThe native microenvironment of mesenchymal stem cells (hMSCs)—the extracellular matrix (ECM), is a complex and heterogenous environment structured at different scales. The present study aims at mimicking the hierarchical microorganization of proteins or growth factors within the ECM using the photolithography technique. Polyethylene terephthalate substrates were used as a model material to geometrically defined regions of RGD + BMP‐2 or RDG + OGP mimetic peptides. These ECM‐derived ligands are under research for regulation of mesenchymal stem cells osteogenic differentiation in a synergic manner. The hMSCs osteogenic differentiation was significantly affected by the spatial distribution of dually grafted peptides on surfaces, and hMSCs cells reacted differently according to the shape and size of peptide micropatterns. Our study demonstrates the presence of a strong interplay between peptide geometric cues and stem cell differentiation toward the osteoblastic lineage. These tethered surfaces provide valuable tools to investigate stem cell fate mechanisms regulated by multiple ECM cues, thereby contributing to the design of new biomaterials and improving hMSCs differentiation cues.
RASSF1 promotes cardiomyocyte apoptosis after acute myocardial infarction and is regulated by miR‐125b
Authors: Xiaochuan Bai , Qianfeng Jiang , Min Xu , Xiao Liang 
Published: October 2019

AbstractCardiomyocyte apoptosis is a common pathological injury in association with acute myocardial infarction (AMI). In the current study, the relationship between Ras‐association domain family 1 (RASSF1) and cardiomyocyte apoptosis was investigated. RASSF1 was significantly over expressed in infarcted myocardial tissues as well as in cardiomyocytes induced by hypoxia. Inhibition of RASSF1 expression alleviated cardiomyocytes apoptosis induced by hypoxia in vitro and reduced cardiomyocytes apoptosis after AMI in vivo. RASSF1 expression was directly modulated by miR‐125b, which was further confirmed by luciferase reporter assay. The current study verified that the miR‐125b/RASSF1 axis was involved in cardiomyocytes apoptosis. To sum up, these results suggest that RASSF1 downregulation alleviated infarction‐induced cardiomyocytes apoptosis and was regulated by miR‐125b.
The posttranslational modification of HDAC4 in cell biology: Mechanisms and potential targets
Authors: Hou Fei , Wei Wei , Qin Xiao , Liang Jing , Han Sha , Han Aizhong , Kong Qingsheng 
Published: October 2019

AbstractHistone deacetylase 4 (HDAC4) is a member of the HDACs family, its expression is closely related to the cell development. The cell is an independent living entity that undergoes proliferation, differentiation, senescence, apoptosis, and pathology, and each process has a strict and complex regulatory system. With deepening of its research, the expression of HDAC4 is critical in the life process. This review focuses on the posttranslational modification of HDAC4 in cell biology, providing an important target for future disease treatment.
SNP rs710886 A>G in long noncoding RNA PCAT1 is associated with the risk of endometriosis by modulating expression of multiple stemness‐related genes via microRNA‐145 signaling pathway
Authors: Wang Liming , Xing Qi , Feng Tongfu , He Ming , Yu Weixu , Chen Hui 
Published: October 2019

AbstractMiR‐145 has been shown to suppress cell invasiveness and proliferation in endometriosis, whereas prostate cancer‐associated transcript 1 (PCAT1) was reported to act as a sponge of miR‐145 with one single‐nucleotide polymorphism (SNP), rs710886, located in the chromosomal segment of PCAT1. Therefore, this study aimed to explore the association between rs710886 SNP and the risk of endometriosis, as well as the effect of this SNP on the activation of the signaling pathway downstream of PCAT1. Real‐time polymerase chain reaction (PCR) was performed to observe the expression of miR‐145 in transfected cells, while Matrigel invasion chamber assays and MTT assay were conducted to examine the invasiveness/proliferation among different cell groups. Moreover, bioinformatics tools, luciferase assays, real‐time PCR, and Western blot analysis were used to measure the expression of these target genes in the presence of miR‐145. Finally, a statistical analysis was conducted to compare the genotypes of rs710886 SNP between fertile healthy women and infertile women with endometriosis. PCAT1 small interfering RNA (siRNA) evidently increased the expression of miR‐145 but reduced the invasiveness/proliferation of cells. P‐PCAT1 exhibited an opposite effect as that of PCAT1 siRNA, indicating PCAT1 could promote the proliferation and invasiveness of endometriosis stem cells via inhibiting the expression of miR‐145. Meanwhile, FASCIN1, SOX2, MSI2, SERPINE1, and JAM‐A were identified as target genes of miR‐145 via computational analysis and luciferase assays. Finally, a significant genetic effect was observed in both the dominant (AG+GG vs AA) and recessive models (GG vs AG+AA), indicating the presence of an association between the genotype of SNP rs710886 and the risk of endometriosis. SNP rs710886 A>G could lower the expression of PCAT1, thus leading to the overexpression of miR‐145. Highly expressed miR‐145 would inhibit the invasiveness and proliferation of endometriosis stem cells via targeting specific genes, thus decreasing the risk of endometriosis.
Leisure behavior and sense of coherence in the context of depression
Authors: Nagata Shinichi , McCormick Bryan , Piatt Jennifer 
Published: October 2019

AbstractAimsImproved sense of coherence (SOC) can protect health among individuals with depression. Literature suggests that leisure behavior can improve SOC, yet empirical studies are lacking. The current study aimed to examine whether leisure behavior was associated with SOC and depression.MethodsA cross‐sectional online survey method was used with a sample of community‐living adults (62% female, age 18–75 years) with a self‐reported current or historical diagnosis of depression recruited from four different depression‐related populations.ResultsThe results of structural equation modeling found that leisure behavior predicted greater SOC as well as lower depressive symptoms. Greater SOC was associated with lower perceived stress and lower depressive symptoms. The total effect size, including both direct and indirect association between leisure behavior and depressive symptoms was 0.452.ConclusionThe findings suggested the potential benefits of leisure behavior on SOC and depressive symptoms.
Gestational diabetes mellitus affects odontoblastic differentiation of dental papilla cells via Toll‐like receptor 4 signaling in offspring
Authors: Lyu Yun , Jia Sixun , Wang Shikang , Wang Tao , Tian Weidong , Chen Guoqing 
Published: October 2019

AbstractGestational diabetes mellitus (GDM) is an important factor involved in the pathogenesis of organ development in the offspring. Here, we analyzed the effects of GDM on odontoblastic differentiation of dental papilla cells (DPCs) and dentin formation in offspring and investigated their underlying mechanisms. A GDM rat model was induced by intraperitoneal injection of streptozotocin and offspring were collected. The results showed that GDM significantly affected odontoblast differentiation and dentin formation in offspring tooth. GDM activated the toll‐like receptor 4 (TLR4)/nuclear factor‐kappa B (NF‐ĸB) signaling pathway and inhibited SMAD1/5/9 signaling to modulate the odontoblastic differentiation of DPCs in offspring. Inhibition of TLR4 signaling by treated with TAK‐242 significantly reverses the suppression of odonto‐differentiation of DPCs in diabetic offspring. Taken together, these data indicate GDM activated the offspring DPCs TLR4/NF‐ĸB signaling, which suppressed the SMAD1/5/9 phosphorylation and then inhibited odontoblasts differentiation and dentin formation.
Cellular reprogramming by single‐cell fusion with mouse embryonic stem cells in pig
Authors: Fang Yuan , Guo Jia , Wu Shuang , Li Xuechun , Zhao Jianchao , Li Yan , Guo Shimeng , Mu Yanshuang , Kong Qingran , Liu Zhonghua 
Published: October 2019

AbstractFusion of differentiated somatic cells with pluripotent stem cells can be used for cellular reprogramming, but the efficiency to obtain hybrid cells is extremely low. Here, we explored a novel cell fusion system, termed single‐cell fusion, the efficiency was significantly improved verified by fusion of mouse embryonic stem cells (mESCs), comparing to traditional polyethylene glycol fusion. Then, we employed the optimized system to perform cell fusion of porcine embryonic fibroblasts (PEFs) and porcine pluripotent stem cells (pPSCs) with mESCs. The hybrid cells showed both red and green fluorescence and expressed species‐specific genes of mouse and pig to evidence that the fusion was successful. The hybrid cells displayed characteristics similar with mESCs, including colony morphology, alkaline phosphatase positive and formation of embryoid body, and the expressions of core pluripotent factors OCT4, NANOG, and SOX2 of the pig were induced in the mESC/PEF hybrid cells. The results indicate PEFs and pPSCs could be reprogrammed by mESCs via the single‐cell fusion. Taking advantage of the hybrid cells to investigate the signaling pathways depended on the pluripotency of pig, we suggest the transforming growth factor‐β signaling pathways may play important roles. In summary, the single‐cell fusion is highly efficient, and we believe in the future it will be widely used in the application and fundamental research.
Gastrointestinal ultrasound in inflammatory bowel disease care: Patient perceptions and impact on disease‐related knowledge
Authors: Rajagopalan Arvind , Sathananthan Dharshan , An Yoon‐Kyo , Van De Ven Lucinda , Martin Serena , Fon James , Costello Samuel P , Begun Jakob , Bryant Robert V 
Published: October 2019

AbstractBackground and AimObjective monitoring of disease activity is integral to therapeutic decision‐making in inflammatory bowel disease (IBD). Data are sparse on patients' perspectives of tools used to monitor disease activity in IBD. To evaluate patients' perspectives of gastrointestinal ultrasound (GIUS) performed during routine IBD clinical care, along with its impact on IBD‐specific knowledge.MethodsPatients with a formal diagnosis of IBD who underwent GIUS at two tertiary IBD services between March 2017 and January 2019 participated in this prospective study. Participants completed a questionnaire measuring the acceptability, tolerability, and usefulness of GIUS using a visual analogue scale (VAS) from 0 (disagree) to 10 (strongly agree). Comparative acceptability of IBD monitoring tools and the impact of GIUS on IBD‐specific knowledge was measured.ResultsA total of 121 participants completed the questionnaire, with a mean age of 42 years (range 17–78), 54 (45%) males, and 79 (65%) Crohn's disease patients. In the overall population, GIUS was scored as highly acceptable for monitoring IBD (mean 9.20 ± 1.37) compared to colonoscopy (7.94 ± 2.30), stool sampling (8.17 ± 1.96), blood sampling (8.87 ± 1.62), and imaging (8.67 ± 1.60); P < 0.01 for each comparison. GIUS caused little patient discomfort (1.88 ± 1.83), and 98 (81%) participants ranked GIUS as their preferred IBD monitoring tool. GIUS also improved patients' overall IBD‐specific knowledge (VAS IBD‐specific knowledge 7.96 ± 1.92), including their understanding of the need for medical therapy and disease extent.ConclusionGIUS is a highly acceptable and well‐tolerated tool for monitoring disease activity in IBD patients. GIUS is preferred by patients and enhances IBD‐specific knowledge.
The role of Lys2–Cl−–Lys2 salt linkages in oligomeric intermediates of RbsD protein in Escherichia coli
Authors: Zheng Jing , Zheng Jie , Ma Yuanwu , Zuo Guocai , Feng Yongjun 
Published: October 2019

AbstractAs a homo‐oligomeric protein, the disassembly of Escherichia coli RbsD decamer produces a urea‐unfolded oligomeric intermediate structure, as the dissociation speed of the protein is lower than that of the unfolding process. There are five Lys2–Cl−–Lys2 salt linkages to connect these subunits. To explore the role of the salt linkages in these oligomeric intermediates, the Lys2Ala mutated in the N‐terminal of E. coli RbsD protein subunit was designed. It was found that the RbsD mutation protein (RbsD:K2A) loses its minor larger oligomers, which exist in RbsD, and displays other several oligomeric states (less than decamers), meanwhile the state of the oligomers depends on the protein concentration. It was also found that compared with RbsD, the crosslinking capability of the subunits of RbsD:K2A is weaker, while the crosslinking rate of dimers is higher, RbsD:K2A needs to substantially adjust its conformation to meet the space requirements when combined with d‐ribose. On the basis of these results, we suggest that Lys2–Cl−–Lys2 salt linkages in E. coli RbsD protein play an important role in stabilizing the intermediate products of oligomers and maintaining interaction between the intermediate products of oligomers, which may shed light on the study of these oligomeric proteins.
Interactions of tea polyphenols with intestinal microbiota and their implication for anti‐obesity
Authors: Zhao Yuan , Zhang Xin 
Published: October 2019

AbstractTea polyphenols (TP) are the main components in tea. Studies in vitro have shown they have significant biological activity; however, the results are inconsistent with experiments in vivo. For the low bioavailability, most TP are thought to remain in the gut and metabolized by intestinal bacteria. In the gut, the unabsorbed TP are metabolized to a variety of derivative products by intestinal flora, which may accumulate to exert beneficial effects. Numerous studies have shown that TP can inhibit obesity and its related metabolism disorders effectively. Meanwhile, it has demonstrated that TP and their derivatives may modulate intestinal micro‐ecology. The understanding of the interaction between TP and intestinal microbiota will allow us to better evaluate the contribution of microbial metabolites of TP to anti‐obesity activity. This review showed implications for the use of TP as functional food with potential therapeutic utility against obesity by modulating intestinal microbiota, contributing to the improvement of human health. © 2019 Society of Chemical Industry
Valorization of snow crab (Chionoecetes opilio) cooking effluents for food applications
Authors: Tremblay Ariane , Corcuff Ronan , Goulet Charles , Godefroy Samuel B , Doyen Alain , Beaulieu Lucie 
Published: October 2019

AbstractBACKGROUNDSeafood processing generates significant amounts of solid and liquid waste in the environment. Such waste represents a potential source of high‐value biomolecules for food, pharmaceutic and cosmetic applications. There are very few studies on the valorization of wastewaters compared to solid by‐products. However, cooking waters are characterized by a high organic polluting load, which could contain valuable molecules such as proteins, pigments and flavor compounds. Snow crab (Chionoecetes opilio) processing is included among the most important processes in Canadian fisheries, although its cooking effluent composition is not well characterized.RESULTSThe present study concentrated and valorized the biomass in snow crab cooking wastewaters for the development of products for food applications. A membrane process was designed and optimized to concentrate the effluents. The chemical composition of the concentrates was analyzed, including characterizing the flavor profile compounds. The extracts were mainly composed of proteins (592 g kg−1) and minerals (386 g kg−1) and contained desirable flavor compounds. Their functional properties (solubility, water‐holding capacity, oil‐holding capacity) and antioxidant activities were also assessed, and their safety was verified.CONCLUSIONThe cooking effluents generated by snow crab processing facilities, usually considered as waste, can be concentrated and turned into a natural aroma for the food industry. © 2019 Society of Chemical Industry