bluuebunny/crossref_metadata_2025_split huggingface dataset:
PET and CSF amyloid-β status are differently predicted by patient features: Information from discordant cases
Authors: Reimand Juhan , de Wilde Arno , Teunissen Charlotte E. , Zwan Marissa , Windhorst Albert D. , Boellaard Ronald , Barkhof Frederik , van der Flier Wiesje M. , Scheltens Philip , van Berckel Bart N.M. , Ossenkoppele Rik , Bouwman Femke 
Published: June 2019

ABSTRACTBackgroundAmyloid-β PET and CSF Aβ42 yield discordant results in 10-20% of patients, possibly providing unique information. Although the predictive power of demographic, clinical, genetic and imaging features for amyloid-positivity has previously been investigated, it is unknown whether these features differentially predict amyloid-β status based on PET or CSF, or whether this differs by disease stage.MethodsWe included 768 patients (subjective cognitive decline (SCD, n=194), mild cognitive impairment (MCI, n=127), dementia (AD and non-AD, n=447) with amyloid-β PET and CSF Aβ42 measurement within one year. 97(13%) patients had discordant PET/CSF amyloid-β status. We performed parallel random forest models predicting separately PET and CSF status using 17 patient features (demographics, APOE4 positivity, CSF (p)tau, cognitive performance, and MRI visual ratings) in the total patient group and stratified by syndrome diagnosis. Thereafter, we selected features with the highest variable importance measure (VIM) as input for logistic regression models, where amyloid status on either PET or CSF was predicted by (i) the selected patient feature, and (ii) the patient feature adjusted for the status of the other amyloid modality.ResultsAPOE4, CSF tau and p-tau had highest VIM for PET and CSF in all groups. In the amyloid-adjusted logistic regression models, p-tau was a significant predictor for PET-amyloid in SCD (OR=1.02[1.01-1.04], pFDR=0.03), MCI (OR=1.05[1.02-1.07], pFDR<0.01) and dementia (OR=1.04[1.03-1.05], pFDR<0.001), but not for CSF-amyloid. APOE4 (OR=3.07[1.33-7.07], punc<0.01) was associated with CSF-amyloid in SCD, while it was only predictive for PET-amyloid in MCI (OR=9.44[2.93,30.39], pFDR<0.01). Worse MMSE scores (OR=1.21[1.03-1.41], punc=0.02) were associated to CSF-amyloid status in SCD, whereas worse memory (OR=1.17[1.05-1.31], pFDR=0.02) only predicted PET positivity in dementia.ConclusionAmyloid status based on either PET or CSF was predicted by different patient features and this varied by disease stage, suggesting that PET-CSF discordance yields unique information. The stronger associations of both APOE4 carriership and worse memory z-scores with CSF-amyloid in SCD suggests that CSF-amyloid is more sensitive early in the disease course. The higher predictive value of CSF p-tau for a positive PET scan suggests that PET is more specific to AD pathology. These findings can influence the choice between amyloid biomarkers in future studies or trials.
The E3 ubiquitin ligase Pib1 regulates effective gluconeogenic shutdown inS. cerevisiae
Authors: Vengayil Vineeth , Laxman Sunil 
Published: June 2019

AbstractCells use multiple mechanisms to regulate their metabolic states depending on changes in their nutrient environment. A well-known example is the response of cells to glucose availability. InS. cerevisiaecells growing in glucose-limited medium, the re-availability of glucose leads to the downregulation of gluconeogenesis, the activation of glycolysis, and robust ‘glucose repression’. However, our knowledge of the initial mechanisms mediating this glucose-dependent downregulation of the gluconeogenic transcription factors is incomplete. We used the gluconeogenic transcription factor Rds2 as a candidate with which to discover regulators of early events leading to glucose repression. Here, we identify a novel role for the E3 ubiquitin ligase Pib1 in regulating the stability and degradation of Rds2. Glucose addition to glucose-limited cells results in rapid ubiquitination of Rds2, followed by its proteasomal degradation. Throughin vivoandin vitroexperiments, we establish Pib1 as a ubiquitin E3 ligase that regulates Rds2 ubiquitination and stability. Notably, this Pib1 mediated Rds2 ubiquitination, followed by proteasomal degradation, is specific to the presence of glucose. Pib1 is required for complete glucose repression, and enables cells to optimally grow in competitive environments when glucose becomes re-available. Our results reveal the existence of a Pib1 E3-ubiquitin ligase mediated regulatory program that mediates glucose-repression when glucose availability is restored.
Doxorubicin-Induced p53 Interferes with Mitophagy in Cardiac Fibroblasts
Authors: Mancilla TR , Aune GJ 
Published: June 2019

AbstractDoxorubicin is a mainstay in pediatric chemotherapy treatment because of its efficacy treating leukemia and lymphoma. Unfortunately, every childhood cancer survivor will develop a chronic health problem, one of the most serious being cardiac disease. How doxorubicin damages the heart in such a way that disease progression occurs over multiple decades is still not understood.The dose of doxorubicin selected does not cause apoptosis but does arrest cell cycle. It also decreases the cells ability to migrate. Gene profiling indicated a cardiac remodeling and inflammatory profile. Mitochondria increased ROS production and underwent membrane depolarization. Secondly, the Parkin:p53 interaction mechanism was investigated. Doxorubicin was found to increase p53 expression and it was shown to sequester Parkin. As a result, mitophagy in doxorubicin-treated cells was decreased. Lastly, cardiac fibroblasts were isolated from p53-/- mice and treated with doxorubicin. The gene expression phenotype in these cells was attenuated and migration was restored. Proliferation was still decreased. Mitochondrial dysfunction was also partially attenuated. Without p53, Parkin could now localize to the mitochondria and mitophagy was restored.Doxorubicin induces a deleterious phenotype in cardiac fibroblasts that may be due to the interaction between two stress responses caused by doxorubicin’s DNA and mitochondrial damage. Cardiac fibroblasts are a viable target and further research needs to be done to elucidate other harmful mechanisms at play in the fibroblast. Knowledge about the importance of cardiac fibroblasts in the development of doxorubicin-induced cardiotoxicity and a pathological mechanism broadens our understanding and ability to develop protective therapies to improve the quality of life of cancer survivors.The project described was supported by all of the following sources for GJA:
St. Baldrick’s Foundation Scholar (Career Development Award)Turn it Gold Foundation
The project described was supported by all of the following sources for TRM:NIH T32GM113896 (STX-MSTP) awardNational Center for Advancing Translational Science, NIH through grant TL1 TR001119. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.
Unique Epigenetic Programming Distinguishes Regenerative Spermatogonial Stem Cells in the Developing Mouse Testis
Authors: Cheng Keren , Chen I-Chung , Hale Benjamin J. , Hermann Brian P. , Geyer Christopher B. , Oatley Jon M. , McCarrey John R. 
Published: June 2019

AbstractSpermatogonial stem cells (SSCs) both self-renew and give rise to progenitor spermatogonia that enter steady-state spermatogenesis in the mammalian testis. However, questions remain regarding the extent to which SSCs and progenitors represent stably distinct spermatogonial subtypes. Here we provide the first multiparametric integrative analysis of mammalian germ cell epigenomes comparable to that done by the ENCODE Project for >100 somatic cell types. Differentially expressed genes distinguishing SSCs and progenitors showed distinct histone modification patterns as well as differences in distal intergenic low-methylated regions. Motif-enrichment analysis predicted transcription factors that regulate this spermatogonial subtype-specific epigenetic programming, and gene-specific chromatin immunoprecipitation analyses confirmed subtype-specific differences in binding of a subset of these factors to target genes. Collectively, these results suggest that SSCs and progenitors are stably distinct spermatogonial subtypes differentially programmed to either self-renew and maintain regenerative capacity as SSCs, or lose regenerative capacity and initiate lineage commitment as progenitors.
Switching between bacteriostatic and bactericidal antimicrobials for retreatment of bovine respiratory disease (BRD) relapses is associated with an increased frequency of resistant pathogen isolation from veterinary diagnostic laboratory submissions
Authors: Coetzee Johann F. , Magstadt Drew R. , Follett Lendie , Sidhu Pritam K. , Schuler Adlai M. , Krull Adam C. , Cooper Vickie L. , Engelken Terry J. , O’Connor Annette M. 
Published: June 2019

AbstractAlthough 90% of BRD relapses are reported to receive retreatment with a different class of antimicrobial, studies examining the impact of antimicrobial selection (i.e. bactericidal or bacteriostatic) on retreatment outcomes and the emergence of antimicrobial resistance (AMR) are deficient in the published literature. A survey was conducted to determine the association between antimicrobial class selection for retreatment of BRD relapses on antimicrobial susceptibility of Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni. Pathogens were isolated from samples submitted to the Iowa State University Veterinary Diagnostic Laboratory from January 2013 to December 2015. A total of 781 isolates with corresponding animal case histories, including treatment protocols, were included in the analysis. Original susceptibility testing of these isolates for ceftiofur, danofloxacin, enrofloxacin, florfenicol, oxytetracycline, spectinomycin, tilmicosin, and tulathromycin was performed using Clinical and Laboratory Standards Institute guidelines. Data were analyzed using a Bayesian approach to evaluate whether retreatment with antimicrobials of different mechanistic classes (bactericidal or bacteriostatic) increased the probability of resistant BRD pathogen isolation in calves. The posterior distribution we calculated suggests that an increased number of treatments is associated with a greater probability of isolates resistant to at least one antimicrobial. In addition, the frequency of resistant M. haemolytica isolates was greater with retreatment using antimicrobials of different mechanistic classes than retreatment with the same class. Specifically, treatment protocols using a bacteriostatic drug first followed by retreatment with a bactericidal drug was associated with a higher frequency of resistant BRD pathogen isolation. This effect was more profound with specific treatment combinations; tulathromycin (bacteriostatic) followed by ceftiofur (bactericidal) was associated with the highest probability of resistant isolates among all antimicrobial combinations. These findings suggest that the selection of antimicrobial mechanistic class for retreatment of BRD should be considered as part of an antimicrobial stewardship program.
Circulating Levels of Free 25(OH)D Increase at the Onset of Rheumatoid Arthritis
Authors: Anaparti Vidyanand , Meng Xiaobo , Mahadevappa Hemsekhar , Smolik Irene , Mookherjee Neeloffer , El-Gabalawy Hani 
Published: June 2019

ABSTRACTObjectiveEpidemiological studies suggest vitamin D deficiency as a potential risk factor for rheumatoid arthritis (RA) development, a chronic autoimmune disorder highly prevalent in indigenous North American (INA) population. We therefore profiled the circulating levels of 25-hydroxyvitaminD [25(OH)D], an active metabolite of vitamin D, in a cohort of at-risk first-degree relatives (FDR) of INA RA patients, a subset of whom subsequently developed RA (progressors).Methods2007 onward, serum samples from INA RA patients and FDR were collected at the time of a structured baseline visit and stored at −20°C. Anti-citrullinated protein antibodies (ACPA), 25(OH)D, hs-CRP, vitamin-D binding protein (VDBP) levels were determined using ELISA and rheumatoid factor (RF) seropositivity was determined by nephelometry.ResultsWe demonstrate that 25 (OH) D concentrations were lower in winter than summer (P=0.0538), and that serum 25(OH)D levels were higher in samples collected and stored after 2013 (P<0.0001). Analysis of samples obtained after 2013 demonstrated that 37.6% of study participants were 25(OH)D insufficient (<75nmol/L). Also, seropositive RA patients and FDR had lower 25(OH)D levels compared to ACPA-/FDR (P<0.05, P<0.01 respectively). Linear regression analysis showed 25(OH)D insufficiency was inversely associated with presence of RA autoantibodies. Longitudinal samples from 14 progressors demonstrated a consistent increase in 25(OH)D levels at the time they exhibited clinically detectable joint inflammation, without any significant change in VDBP levels.ConclusionWe demonstrate that 25(OH)D levels in serum increased at RA onset in progressors. The potential role that vitamin D metabolites and their downstream effects play in RA transition requires further investigation.
Andropogon bicornis L. (Poales: Poaceae): a stink bug shelter in the soybean and corn off-season in southern Brazil
Authors: Engel Eduardo , Batistella Pasini Mauricio Paulo , Zamberlan João Fernando , Bortolotto Rafael Pivotto , Horn Roberta Cattaneo , Camera Juliane Nicolodi , Hörz Daniele Caroline 
Published: June 2019

AbstractAssociated host plants promote the survival of various species of pest insects during unfavorable periods. The objective of this study was to evaluate the diversity, abundance and structure of the pentatomid bugs community during the soybean and corn off-season in Andropogon bicornis L. (Poales, Poaceae) plants. The experiment was carried out in the municipality of Cruz Alta, RS, Brazil. During the soybean and corn off-season from 2014 to 2018, clumps of 10 to 50 centimeters in diameter were observed around the growing area. Data on the number of species and abundance of individuals were used for statistical analysis (ANOVA, linear regression and Pearson correlation) and faunistic (diversity and abundance distribution). At the end of the experiment 4050 adults belonging to the species Euschistus heros (F.), Dichelops furcatus (F.), Dichelops melacanthus (Dallas), Edessa meditabunda (F.), Edessa ruformaginata (De Geer) and Piezodorus guildini. Among the species, we found greater abundance for E. meditabunda, E. heros and D. furcatus (96.07%) of the individuals sampled. Higher interspecific correlation was observed between the same species, we also observed a direct effect of the clump diameter on the population density. Among the six species observed, at least five are economically important for soybean and corn crops, so further studies are needed in order to verify the effects of this hibernation site on the population present in the crop and its damages.
Significant causes of claims and disputes in construction projects in Saudi Arabia
Authors: Assaf Sadi , Hassanain Mohammad A. , Abdallah Abdullatif , Sayed Ahmed M.Z. , Alshahrani Abdulrahman 
Published: June 2019

Purpose
Projects in the construction industry have been confronted with an unprecedented volume of claims, which negatively impact the construction business environment. The purpose of this paper is to identify and assess the major causes of claims and disputes, from the perspectives of three key stakeholders.

Design/methodology/approach
An online and paper-based questionnaire was distributed to a random sample of 250 professionals. Responses from 86 construction professionals were obtained. The significance index was employed to rank the factors and hence assess their relative significance. Results were analyzed from both the individual stakeholder, and collective perspectives. Test of agreement was also conducted.

Findings
The research found the following causes to be the most significant contributors to claims and disputes in Saudi Arabia: “change or variation orders due to new requirements from client” (78 percent), “variations in quantities due to new requirements from client” (74 percent), “delay caused by contractor” (74 percent), “design errors or omissions” (72 percent) and “inconsistencies in the drawings and specifications” (70 percent).

Originality/value
The research provides an in-depth assessment of the causes of claims and disputes, in the setting of a fast growing industry, in which strong performance is essential to continued success. Unlike several of the reviewed studies which reported “delay caused by the owner” or “delayed in payment” as a major cause of claims and/or disputes, the findings of the current study indicated that this cause was not the most significant. Instead, it was determined that the top causes of claims and disputes were attributed to all parties, including consultants, contractors and clients.
Critical reflections on quality standards within drug demand reduction
Authors: Graf Niels , Stöver Heino 
Published: June 2019

Purpose
The purpose of this paper is to critically reflect on the usefulness of quality standards aimed at prevention interventions for drug using young offenders.

Design/methodology/approach
This paper uses critical literature on quality standards, readings of quality standards and qualitative interviews as well as focus-group discussions with professionals working in services targeting drug use among young offenders.

Findings
The findings show discrepancies between the idea that quality standards provide a tool for supporting the implementation of more effective interventions and professionals’ experiences with quality standards as almost absent in their work.

Originality/value
This viewpoint highlights barriers to the implementation of quality standards that have to be overcome if quality standards are to be adopted and implemented in practice.
Reconfiguration method of tensegrity units using infinitesimal mechanisms
Authors: González Andrés , Luo Ani , Shi Dongyan 
Published: June 2019

Purpose
This paper aims to present a reconfiguration strategy for actuated tensegrity structures. The main idea is to use the infinitesimal mechanisms of the structure to generate a path along which the tensegrity can change its shape while maintaining the equilibrium.

Design/methodology/approach
Combining the force density method with a marching procedure, the solution to the equilibrium problem is given by a set of differential equations. Beginning from an initial stable position, the algorithm calculates a small displacement until a new stable configuration is reached, and recurrently repeats the process during a given interval of time.

Findings
By means of three numerical simulations and their respective experimental example, the efficacy of this algorithm for reconfiguring the well-known three-bar tensegrity prism along different directions is shown. The proposed method shows efficiency only for small changes of string length. Further work should consider the application of this method to more complex tensegrity structures.

Originality/value
The advantage of this reconfiguration method is its simplicity for finding new stable positions for tensegrity structures, and the fact that it doesn’t need the information of the material of the structure for the computations.
Comparison of training methods with ELECTRE I and Merrill’s principles
Authors: Jghamou Afaf , Maziri Aziz , Mallil El Hassan , Echaabi Jamal 
Published: June 2019

Purpose
In this paper, the authors focus on training as a frequently used knowledge management tool. This paper aims to help training function to achieve excellence at the first attempt by evaluating and deciding on the most interesting method for each training action before engaging the investment.

Design/methodology/approach
The authors apply instructional theories to evaluate the relevance of training methods and explored the multiple criteria decision analysis methods, which is a mathematical approach, for the evaluation to be rational. An experimental research based on study cases is also presented to test the applicability and effectiveness of the model proposed.

Findings
A decisional model that allows to choose rationally the most appropriate training method for each case. It is based on Elicitation and Choice Translating Reality (ELECTRE I) method, which is a multi-criteria decision analysis method and uses criteria from First Principles developed by Merrill in 2002.

Practical implications
The proposed model may have several implications for the improvement of training performance, particularly in the context of quality management systems that require product compliance based on continuous improvement and risk-based approaches. It can, therefore, be used as a tool to control the quality of training process or control the risk relative to the execution of a training action or more generally as a tool to “check” that training methods chosen are the most appropriate to attempt the training objectives before “act” the training action.

Originality/value
The combination of a decision analysis system with the theory of instruction and the applicability to training process management.
Conjoint analysis of consumer preferences for dress design
Authors: Zhou Xiaoxi , Xu Yunhao 
Published: June 2019

Purpose
In the process of designing new clothes, designers should identify specific user groups’ preferences and attitudes toward certain types of design, ascertain the design elements that make clothes popular in the market, and combine these elements to devise the best clothing design scheme. The purpose of this paper is to discover which design elements influence dress purchases and how age affects consumers’ choices in regard to these elements.

Design/methodology/approach
This study uses conjoint analysis in dress design to provide an effective method for designers to identify consumers’ preferences. First, the important attributes and attribute levels of dress design were determined. Next, the experimental samples for the attitude measurement chart were generated by orthogonal design. Finally, the data of 318 samples were analyzed by conjoint analysis to determine consumers’ preferences.

Findings
The results revealed that the “silhouette” attribute is the most important decision criterion for dress purchase, followed by the “dress length” attribute. In contrast, the “waistline height” attribute is perceived as least important. The study also identified the dress design features’ preferences of consumers of different ages. According to the results of the analysis, user groups’ preferences and acceptability regarding different design features were revealed, and the favorite dress design portfolio for age-specific consumers was obtained.

Originality/value
Currently, there is little information in the literature about consumers’ preferences regarding dress design. In this study, the use of conjoint analysis reveals and visualizes complex statistical results. This research approach is also applicable to the design and decision-making processes used for other apparel, and it can help designers better incorporate different users’ needs into clothing design.