bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bexarotene – a novel modulator of AURKA and the primary cilium in VHL-deficient cells
Authors: Chowdhury Pratim , Powell Reid T. , Stephan Clifford , Uray Ivan P. , Talley Tia , Karki Menuka , Tripathi Durga Nand , Park Yong Sung , Mancini Michael A. , Davies Peter , Dere Ruhee 
Published: December 2018

ABSTRACT
Loss of the gene von Hippel–Lindau (VHL) is associated with loss of primary cilia and is causally linked to elevated levels of Aurora kinase A (AURKA). We developed an image-based high-throughput screening (HTS) assay using a dual-labeling image analysis strategy that identifies both the cilium and the basal body. By using this strategy, we screened small-molecule compounds for the targeted rescue of cilia defects associated with VHL deficiency with high accuracy and reproducibility. Bexarotene was identified and validated as a positive regulator of the primary cilium. Importantly, the inability of an alternative retinoid X receptor (RXR) agonist to rescue ciliogenesis, in contrast to bexarotene, suggested that multiple bexarotene-driven mechanisms were responsible for the rescue. We found that bexarotene decreased AURKA expression in VHL-deficient cells, thereby restoring the ability of these cells to ciliate in the absence of VHL. Finally, bexarotene treatment reduced the propensity of subcutaneous lesions to develop into tumors in a mouse xenograft model of renal cell carcinoma (RCC), with a concomitant decrease in activated AURKA, highlighting the potential of bexarotene treatment as an intervention strategy in the clinic to manage renal cystogenesis associated with VHL deficiency and elevated AURKA expression.
Distinct metabolic adjustments arise from acclimation to constant hypoxia and intermittent hypoxia in estuarine killifish (Fundulus heteroclitus)
Authors: Borowiec Brittney G. , McClelland Grant B. , Rees Bernard B. , Scott Graham R. 
Published: December 2018

ABSTRACT
Many fish experience daily cycles of hypoxia in the wild, but the physiological strategies for coping with intermittent hypoxia are poorly understood. We examined how killifish adjust O2 supply and demand during acute hypoxia, and how these responses are altered after prolonged acclimation to constant or intermittent patterns of hypoxia exposure. We acclimated killifish to normoxia (∼20 kPa O2), constant hypoxia (2 kPa) or intermittent cycles of nocturnal hypoxia (12 h:12 h normoxia:hypoxia) for 28 days, and then compared whole-animal O2 consumption rates (ṀO2) and tissue metabolites during exposure to 12 h of hypoxia followed by reoxygenation in normoxia. Normoxia-acclimated fish experienced a pronounced 27% drop in ṀO2 during acute hypoxia, and modestly increased ṀO2 upon reoxygenation. They strongly recruited anaerobic metabolism during acute hypoxia, indicated by lactate accumulation in plasma, muscle, liver, brain, heart and digestive tract, as well as a transient drop in intracellular pH, and they increased hypoxia inducible factor (HIF)-1α protein abundance in muscle. Glycogen, glucose and glucose-6-phosphate levels suggested that glycogen supported brain metabolism in hypoxia, while the muscle used circulating glucose. Acclimation to constant hypoxia caused a stable ∼50% decrease in ṀO2 that persisted after reoxygenation, with minimal recruitment of anaerobic metabolism, suggestive of metabolic depression. By contrast, fish acclimated to intermittent hypoxia maintained sufficient O2 transport to support normoxic ṀO2, modestly recruited lactate metabolism and increased ṀO2 dramatically upon reoxygenation. Both groups of hypoxia-acclimated fish had similar glycogen, ATP, intracellular pH and HIF-1α levels as normoxic controls. We conclude that different patterns of hypoxia exposure favour distinct strategies for matching O2 supply and O2 demand.
Mapping of microRNAs related to cervical cancer in Latin American human genomic variants
Authors: Guerrero Flórez Milena , Guerrero Gómez Olivia Alexandra , Mena Huertas Jaqueline , Yépez Chamorro María Clara 
Published: December 2018

Background: MicroRNAs are related to human cancers, including cervical cancer (CC) caused by HPV. In 2018, approximately 56.075 cases and 28.252 deaths from this cancer were registered in Latin America and the Caribbean according to GLOBOCAN reports. The main molecular mechanism of HPV in CC is related to integration of viral DNA into the hosts’ genome. However, the different variants in the human genome can result in different integration mechanisms, specifically involving microRNAs (miRNAs). Methods: The miRNAs associated with CC were obtained from literature, the miRNA sequences and four human genome variants (HGV) from Latin American populations were obtained from miRBase and 1000 Genomes Browser, respectively. HPV integration sites near cell cycle regulatory genes were identified. miRNAs were mapped on HGV. miRSNPs were identified in the miRNA sequences located at HPV integration sites on the Latin American HGV. Results: Two hundred seventy-two miRNAs associated with CC were identified in 139 reports from different geographic locations. By mapping with Blast-Like Alignment Tool (BLAT), 2028 binding sites were identified from these miRNAs on the human genome (version GRCh38/hg38); 42 miRNAs were located on unique integration sites; and miR-5095, miR-548c-5p and miR-548d-5p were involved with multiple genes related to the cell cycle. Thirty-seven miRNAs were mapped on the Latin American HGV (PUR, MXL, CLM and PEL), but only miR-11-3p, miR-31-3p, miR-107, miR-133a-3p, miR-133a-5p, miR-133b, miR-215-5p, miR-491-3p, miR-548d-5p and miR-944 were conserved. Conclusions: Ten miRNAs were conserved in the four HGV. In the remaining 27 miRNAs, substitutions, deletions or insertions were observed. These variation patterns can imply differentiated mechanisms towards each genomic variant in human populations because of specific genomic patterns and geographic features. These findings may help in determining susceptibility for CC development. Further identification of cellular genes and signalling pathways involved in CC progression could lead new therapeutic strategies based on miRNAs.
Problems of destructive relations in oficial collectives of organs of internal affairs
Authors: Михайлова Татьяна , Mihaylova Tat'yana , Джонов Айс , Dshonov Ais 
Published: December 2018

Considering the conditions and specifics of work, the constant tension of the labor process, service in the organs of internal affairs is undoubtedly one of the most difficult types of professional activity. Thus, according to the indicators of tension, it belongs to the 3rd class of complexity.
Practically all areas of operational and service activity of the internal Affairs bodies imply collective work, with a large number of employees United by the solution of complex state tasks on protection of life, health, rights and freedoms of citizens of the Russian Federation, foreign citizens, stateless persons, for combating crime, protection of public order, property and for ensuring public safety.
In such a situation, special attention should be paid to the moral and psychological climate, which is formed in one or another staff of the internal Affairs bodies as a result of constant interaction both in office and outside it.
In modern conditions of constant reforming, optimization of structure and regular number of the Ministry of internal Affairs among employees of law-enforcement bodies various negative interrelations which in General can be characterized as destructive are shown.
The need to strengthen the discipline among the employees of the internal Affairs bodies by constantly monitoring the socio-psychological climate in the team and the formation of optimal relationships in the service teams is one of the most important psychological and pedagogical tasks of the entire management of the Ministry of internal Affairs at all levels.
The National Glycohemoglobin Standardization Program: Over 20 Years of Improving Hemoglobin A1c Measurement
Authors: Little Randie R , Rohlfing Curt , Sacks David B 
Published: December 2018

Abstract

BACKGROUND
Measurement of hemoglobin A1c (HbA1c) in the blood is integral to and essential for the treatment of patients with diabetes mellitus. HbA1c reflects the mean blood glucose concentration over the preceding 8 to 12 weeks. Although the clinical value of HbA1c was initially limited by large differences in results among various methods, the investment of considerable effort to implement standardization has brought about a marked improvement in analysis.

CONTENT
The focus of this review is on the substantial progress that has been achieved in enhancing the accuracy and, therefore, the clinical value of HbA1c assays.

SUMMARY
The interactions between the National Glycohemoglobin Standardization Program and manufacturers of HbA1c methods have been instrumental in standardizing HbA1c. Proficiency testing using whole blood has allowed accuracy-based assessment of methods in individual clinical laboratories that has made an important contribution to improving the HbA1c measurement in patient samples. These initiatives, supported by the efforts of the IFCC network, have led to a continuing enhancement of HbA1c methods.
Many of the factors that previously influenced HbA1c results independently of blood glucose have been eliminated from most modern methods. These include carbamylation, labile intermediates, and common hemoglobin variants. Nevertheless, some factors (e.g., race and aging) may alter HbA1c interpretation, but whether these differences have clinical implications remains contentious. HbA1c has a fundamental role in the diagnosis and management of diabetes. Ongoing improvements in HbA1c measurement and quality will further enhance the clinical value of this analyte.
Variable weak Hardy spaces WH
L

p(·)(ℝ
n
) associated with operators satisfying Davies–Gaffney estimates
Authors: Zhuo Ciqiang , Yang Dachun 
Published: December 2018

Abstract
Let

p
⁢

(
⋅
)

:

ℝ
n

→

[
0
,
1
]

{p(\,\cdot\,)\colon\mathbb{R}^{n}\to[0,1]}

be a variable exponent function satisfying the globally log-Hölder continuous condition, and L a one-to-one operator of type ω in

L
2

⁢

(

ℝ
n

)

{L^{2}({\mathbb{R}}^{n})}

, with

ω
∈

[
0
,

π
/
2

)

{\omega\in[0,\pi/2)}

, which has a bounded holomorphic functional calculus and satisfies the Davies–Gaffney estimates.
In this article, we introduce the variable weak Hardy space

WH
L

p
⁢

(
⋅
)

⁢

(

ℝ
n

)

{\mathrm{WH}^{{p(\,\cdot\,)}}_{L}(\mathbb{R}^{n})}

, associated with L via the corresponding square function.
Its molecular characterization is then established by means of the atomic decomposition of the variable weak tent space

WT

p
⁢

(
⋅
)

⁢

(

ℝ
+

n
+
1

)

{\mathrm{WT}^{p(\,\cdot\,)}(\mathbb{R}_{+}^{n+1})}

, which is also obtained in this article.
In particular, when L is non-negative and self-adjoint, we obtain the
atomic characterization of

WH
L

p
⁢

(
⋅
)

⁢

(

ℝ
n

)

{\mathrm{WH}^{{p(\,\cdot\,)}}_{L}(\mathbb{R}^{n})}

.
As an application of the molecular characterization, when L is the second-order divergence form elliptic operator with complex bounded measurable coefficients, we prove that the associated Riesz transform

∇
⁡

L

-

1
/
2

{\nabla L^{-1/2}}

is bounded from

WH
L

p
⁢

(
⋅
)

⁢

(

ℝ
n

)

{\mathrm{WH}^{{p(\,\cdot\,)}}_{L}(\mathbb{R}^{n})}

to the variable weak Hardy space

WH

p
⁢

(
⋅
)

⁢

(

ℝ
n

)

{\mathrm{WH}^{p(\,\cdot\,)}(\mathbb{R}^{n})}

.
Moreover, when L is non-negative and self-adjoint with the kernels of

{

e

-

t
⁢
L

}

t
>
0

{\{e^{-tL}\}_{t>0}}

satisfying the Gaussian upper bound estimates, the atomic characterization of

WH
L

p
⁢

(
⋅
)

⁢

(

ℝ
n

)

{\mathrm{WH}^{{p(\,\cdot\,)}}_{L}(\mathbb{R}^{n})}

is further used to characterize this space via non-tangential maximal functions.
Stabilization of 3D Navier–Stokes–Voigt equations
Authors: Anh Cung The , Tuan Nguyen Viet 
Published: December 2018

Abstract
We consider 3D Navier–Stokes–Voigt equations in smooth bounded domains with homogeneous Dirichlet boundary conditions. First, we study the existence and exponential stability of strong stationary solutions to the problem. Then we show that any unstable steady state can be exponentially stabilized by using either an internal feedback control with a support large enough or a multiplicative Itô noise of sufficient intensity.
An engineered lipocalin that tightly complexes the plant poison colchicine for use as antidote and in bioanalytical applications
Authors: Barkovskiy Mikhail , Ilyukhina Elena , Dauner Martin , Eichinger Andreas , Skerra Arne 
Published: December 2018

Abstract
Colchicine is a toxic alkaloid prevalent in autumn crocus (Colchicum autumnale) that binds to tubulin and inhibits polymerization of microtubules. Using combinatorial and rational protein design, we have developed an artificial binding protein based on the human lipocalin 2 that binds colchicine with a dissociation constant of 120 pm, i.e. 10000-fold stronger than tubulin. Crystallographic analysis of the engineered lipocalin, dubbed Colchicalin, revealed major structural changes in the flexible loop region that forms the ligand pocket at the open end of the eight-stranded β-barrel, resulting in a lid-like structure over the deeply buried colchicine. A cis-peptide bond between residues Phe71 and Pro72 in loop #2 constitutes a peculiar feature and allows intimate contact with the tricyclic ligand. Using directed evolution, we achieved an extraordinary dissociation half-life of more than 9 h for the Colchicalin-colchicine complex. Together with the chemical robustness of colchicine and availability of activated derivatives, this also opens applications as a general-purpose affinity reagent, including facile quantification of colchicine in biological samples. Given that engineered lipocalins, also known as Anticalin® proteins, represent a class of clinically validated biopharmaceuticals, Colchicalin may offer a therapeutic antidote to scavenge colchicine and reverse its poisoning effect in situations of acute intoxication.
Composition and Characteristics of Yam Juice Fermented by Lactobacillus plantarum and Streptococcus thermophilus
Authors: Chen Wenxue , Zhu Jianzhong , Niu Hui , Song Yizhe , Zhang Weimin , Chen Haiming , Chen Weijun 
Published: December 2018

Abstract
In this study, Lactobacillus plantarum (LP), alone or in combination with Streptococcus thermophilus, was used to ferment yam juice. Changes in the composition (phenols, organic acids, reducing sugars and volatile substances) and functional characteristics (antioxidative activity and ability to regulate the intestinal flora) of yam juice during fermentation were investigated. The results showed that the total phenolic (TP) content increased from 201.27 to 281.27 and 285.77 μg/mL for LP- and L. plantarum and S. thermophilus (LPST)-fermented yam juice, respectively. The antioxidative activity of yam juice improved significantly after fermentation, highly correlating with its TP content. In addition, LP- or LPST-fermented yam juice had positive effects on members of the human intestinal flora, improving the activity of Bifidobacterium and inhibiting the growth of Escherichia coli. Sensory analysis showed that LPST-fermented yam juice had a highest score. The results of this study showed that fermented yam juice can serve as a healthy beverage for consumers with low immunity or an imbalance of the intestinal flora.
Exploring the pedagogical potential of vertical and horizontal relations in the constructicon:The case of the family of subjective-transitive constructions withdecirin Spanish
Authors: Gonzálvez-García Francisco 
Published: December 2018

AbstractThis paper explores the pedagogical implications and implementations of a Cognitive Construction Grammar (Goldberg, Adele E. 2006.Constructions at work: The nature of generalization in language. New York: Oxford University Press) approach for the teaching of construal in the L2 class of Spanish at an advanced level. To this end, this paper focuses on instances of secondary predication (involving a direct object and an object-related XPCOMP) withdecir“say” and other verbs of saying and calling in present-day Spanish, under the rubric of the denominative subjective-transitive construction. This construction comprises a number of lower-level configurations involving a reflexive direct object (the reflexive subjective-transitive construction), and an imperative verb (the imperative subjective-transitive construction). The verbdeciris also frequently attested in the reflex passive construction (the impersonal subjective-transitive construction), under which two different, though closely connected, lexically-filledlo que se diceXPCOMP configurations can be posited, which may function as a focusing/emphasizer subjunct or as a summative conjunct in present-day Spanish. A default inheritance system of the type invoked in Cognitive Construction Grammar is shown to capture broad and specific generalizations at a horizontal level (among the verbs attested in the (sub-)construction(s)) and a vertical level (among constructions of varying degrees of specificity) and can thus be informally used to optimize the pedagogical efficiency of the input for the explicit instruction of grammar in the advanced Spanish L2 class.
Invertibility and stability for a generic class of radon transforms with application to dynamic operators
Authors: RabieniaHaratbar Siamak 
Published: December 2018

Abstract
Let X be an open subset of

ℝ
2

{\mathbb{R}^{2}}

.
We study the dynamic operator,

𝒜

{\mathcal{A}}

, integrating over a family of level curves in X when the object changes between the measurement.
We use analytic microlocal analysis to determine which singularities can be recovered by the data-set.
Our results show that not all singularities can be recovered as the object moves with a speed lower than the X-ray source.
We establish stability estimates and prove that the injectivity and stability are of a generic set if the dynamic operator satisfies the visibility, no conjugate points, and local Bolker conditions.
We also show this results can be implemented to fan beam geometry.
A Turkish girl with H syndrome: stunted growth and development of autoimmune insulin dependent diabetes mellitus in the 6th year of diagnosis
Authors: Ozlu Can , Yesiltepe Mutlu Gul , Hatun Sukru 
Published: December 2018

Abstract

Background
H syndrome ([OMIM] 602782) is an autosomal recessive disorder with systemic manifestations and characteristic skin lesions, caused by mutations of the SLC29A3 gene. Short stature and diabetes mellitus are the major endocrine problems related to H syndrome, however, clear data from clinical follow-up of H syndrome patients is lacking in the literature.

Case presentation
Here, we present follow-up of a Turkish girl diagnosed with H syndrome at the age of 10 with a homozygous 310(c.933T>A, p.C310X) early stop codon mutation on exon 6 of the SLC29A3 gene. She had severe short stature non-responsive to growth hormone (GH) treatment and gluten-free diet despite low GH levels and celiac antibody positivity. She developed insulin dependent diabetes mellitus (IDDM) symptoms 6 years after the initial diagnosis.

Conclusions
H syndrome patients can develop IDDM years after characteristic symptoms. Short stature in H syndrome patients may not respond to GH replacement or gluten-free diet alone.
Blade Tip-timing Technology with Multiple Reference Phases for Online Monitoring of High-speed Blades under Variable-speed Operation
Authors: Ji-wang Zhang , Lai-bin Zhang , Ke-Qin Ding , Li-xiang Duan 
Published: December 2018

Abstract
High-speed blades form core mechanical components in turbomachines. Research concerning online monitoring of operating states of such blades has drawn increased attention in recent years. To this end, various methods have been devised, of which, the blade tip-timing (BTT) technique is considered the most promising. However, the traditional BTT method is only suitable for constant-speed operations. But in practice, the rotational speed of turbomachine blades is constantly changing under the influence of external factors, which lead to unacceptable errors in measurement. To tackle this problem, a new BTT method based on multi-phases is proposed. A plurality of phases was arranged as evenly as possible on the rotating shaft to determine the rotation speed. Meanwhile, the corresponding virtual reference point was determined in accordance with the number of blades between consecutive phases. Based on these reference points, equations to measure displacement due to blade vibrations were deduced. Finally, mathematical modeling, numerical simulation and experimental tests were performed to verify the validity of the proposed method. Results demonstrate that the error in measurement induced when using the proposed method is less than 1.8 %, which is much lower compared to traditional methods utilized under variable-speed operation.
Crystal structure of catena-poly[tetraiodido-(μ2-1,4-bis(2-methyl-1H-imidazol-1-yl)benzene-κ2
N:N′)dimercury(II)], C14H14Hg2I4N4
Authors: Hou Bing-Jie , Osman Ibrahim Omar , Hu Dong-Cheng , Liu Jia-Cheng 
Published: December 2018

Abstract
C14H14Hg2I4N4, monoclinic, P21/c, a = 4.3592(11) Å, b = 20.116(5) Å, c = 12.713(3) Å, β = 96.838(5)°, Z = 2, V = 1106.9(5) Å3, R
gt(F) = 0.0362, wR
ref(F
2) = 0.0863, T = 296(2) K.
Crystal structure of ((5,5′-dimethoxy-2,2′-(1,2-phenylenebis(nitrilomethylidyne)))diphenolato-κ4
O,N,O′,N′)copper(II), C22H18N2CuO4
Authors: Ling Zhao , Xin Wang , Liguo Yang , Linna Gao 
Published: December 2018

Abstract
C22H18N2CuO4, monoclinic, P21/n (no. 14), a = 15.7861(11) Å, b = 5.3211(4) Å, c = 21.6563(16) Å, β = 92.129(4)°, V = 1817.9(2) Å3, Z = 4, R
gt(F) = 0.0254, wR
ref(F
2) = 0.0765, T = 298 K.
Crystal structure of bis((E)-2-ethoxy-6-(((2-hydroxyethyl)imino)methyl)phenolato-κ2
N,O)copper(II), C22H28N2CuO6
Authors: Xin Wang , Jinglan Kan , Yonghui Zhang , Liguo Yang , Linna Gao 
Published: December 2018

Abstract
C22H28N2CuO6, monoclinic, P21/c (no. 14), a = 8.5942(7) Å, b = 25.888(2) Å, c = 4.8204(4) Å, β = 97.785(3)°, V = 1062.60(15) Å3, Z = 2, R
gt(F) = 0.0313, wR
ref(F
2) = 0.0878, T = 296(2) K.
Non-Similar Comutational Solutions for Double-Diffusive MHD Transport Phenomena for Non-Newtnian Nanofluid From a Horizontal Circular Cylinder
Authors: Ramachandra Prasad V. , Abdul gaffar S. , Rushi Kumar B. 
Published: December 2018

Abstract
This article aims to study theoretically the combined magneto hydrodynamic flows of casson viscoplastic nanofluid from a horizontal isothermal circular cylinder in non-Darcy porous medium. The impacts of Brownian motion and thermophoresis are consolidated and studied. The governing partial differential equations are converted into nonlinear ordinary differential equations using suitable non-similarity transformation and are solved numerically using Keller-Box finite difference technique. The numerical method is validated with previous published work and the results are found to be in excellent agreement. Numerical results for velocity, temperature, concentration along with skin friction coefficient, heat and mass transfer rate are discussed for various values of physical parameters. It is observed that velocity, heat and mass transfer rate are increased with increasing casson fluid parameter whereas temperature, concentration and skin friction are decreased. Velocity is reduced with increasing Forchheimer parameter whereas temperature and nano-particle concentration are both enhanced. An increase in magnetic parameter is seen to increase temperature and concentration whereas velocity, skin friction heat and mass transfer rate are decreased. The present model finds applications in electric-conductive nano-materials of potential use in aviation and different enterprises, energy systems and thermal enhancement of industrial flow processes.