bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of matrix stiffness on the behavior of brain metastatic breast cancer cells in a biomimetic hyaluronic acid hydrogel platform
Authors: Narkhede Akshay A. , Crenshaw James H. , Manning Riley M. , Rao Shreyas S. 
Published: February 2018

AbstractBreast cancer brain metastasis marks the most advanced stage of breast cancer no longer considered curable with a median survival period of ∼4–16 months. Apart from the genetic susceptibility (subtype) of breast tumors, brain metastasis is also dictated by the biophysical/chemical interactions of tumor cells with native brain microenvironment, which remain obscure, primarily due to the lack of tunable biomimetic in vitro models. To address this need, we utilized a biomimetic hyaluronic acid (HA) hydrogel platform to elucidate the impact of matrix stiffness on the behavior of MDA‐MB‐231Br cells, a brain metastasizing variant of the triple negative breast cancer line MDA‐MB‐231. We prepared HA hydrogels of varying stiffness (0.2–4.5 kPa) bracketing the brain relevant stiffness range to recapitulate the biophysical cues provided by brain extracellular matrix. In this system, we observed that the MDA‐MB‐231Br cell adhesion, spreading, proliferation, and migration significantly increased with the hydrogel stiffness. We also demonstrated that the stiffness based responses of these cells were mediated, in part, through the focal adhesion kinase‐phosphoinositide‐3 kinase pathway. This biomimetic material system with tunable stiffness provides an ideal platform to further the understanding of mechanoregulation associated with brain metastatic breast cancer cells. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1832–1841, 2018.
Effects of substrate stiffness on dental pulp stromal cells in culture
Authors: Datko Williams Laura , Farley Amanda , Cupelli Matthew , Alapati Satish , Kennedy Marian S. , Dean Delphine 
Published: February 2018

AbstractDental pulp stromal cells (DPSCs) can be differentiated down lineages known to either express bone or dentin specific protein markers. Since the differentiation of cells can be heavily influenced by their environment, it may be possible to influence the osteogenic/odontogenic potential of DPSCs by modulating the mechanical properties of substrate on which they are grown. In this study, human DPSCs were grown with and without hydroxyapatite (HA) microparticles on a range of substrates including fibronectin‐coated hydrogels and glass substrates, which represented an elastic moduli range of approximately 3 kPa–50 GPa, over a 21‐day period. Alkaline phosphatase activity, osteopontin production, and mineralization were monitored. The presence of HA microparticles increased the relative degree of mineralized matrix produced by the cells relative to those in the same substrate and media condition without the HA microparticles. In addition, cultures with cells grown on stiffer substrates had higher ALP activity and higher degree of mineralization than those grown on softer substrates. This study shows that DPSCs are affected by the mechanical properties of their underlying growth substrate and by the presence of HA microparticles. In addition, relatively stiff substrates (>75 kPa) may be required for significant mineralization of these cultures. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1789–1797, 2018.
Biotransformation of 4‐fluoro‐N‐(1‐{2‐[(propan‐2‐yl)phenoxy]ethyl}‐8‐azabicyclo[3.2.1]octan‐3‐yl)‐benzenesulfonamide, a novel potent 5‐HT7 receptor antagonist with antidepressant‐like and anxiolytic properties: In vitro and in silico approach
Authors: Słoczyńska Karolina , Wójcik‐Pszczoła Katarzyna , Canale Vittorio , Żmudzki Paweł , Zajdel Paweł , Pękala Elżbieta 
Published: February 2018

AbstractThe aim of the study was to investigate the metabolism of 4‐fluoro‐N‐(1‐{2‐[(propan‐2‐yl)phenoxy]ethyl}‐8‐azabicyclo[3.2.1]octan‐3‐yl)‐benzenesulfonamide (PZ‐1150), a novel 5‐HT7 receptor antagonist with antidepressant‐like and anxiolytic properties, by the following three ways: in vitro with microsomes; in vitro employing Cunninghamella echinulata, and in silico using MetaSite. Biotransformation of PZ‐1150 with microsomes resulted in five metabolites, while transformation with C. echinulata afforded two metabolites. In both models, the predominant metabolite occurred due to hydroxylation of benzene ring. In silico data coincide with in vitro experiments, as three MetaSite metabolites matched compounds identified in microsomal samples. In human liver microsomes PZ‐1150 exhibited in vitro half‐life of 64 min, with microsomal intrinsic clearance of 54.1 μL/min/mg and intrinsic clearance of 48.7 mL/min/kg. Therefore, PZ‐1150 is predicted to be a high‐clearance agent. The study demonstrated the applicability of using microsomal model coupled with microbial model to elucidate the metabolic pathways of compounds and comparison with in silico metabolite predictions.
Inclusion of frequency‐dependent parameters in power transmission lines simulation using harmonic analysis and proper generalized decomposition
Authors: Malik Muhammad Haris , Borzacchiello Domenico , Chinesta Francisco , Diez Pedro 
Published: February 2018

AbstractThis study presents the application of proper generalized decomposition on transmission line models involving frequency‐dependent parameters. Frequency dependence of parameters can be due to a number of reasons including phenomena such as “ground return effects,” “proximity effects,” and “skin effects.” In the current study, simplified methods of skin effects based on Bessel functions are used to showcase the method. Although we implement our study using a specific model for skin effects to demonstrate the effectiveness of the proposed method to accommodate frequency‐dependent effects in proper generalized decomposition, the present work is not meant to discuss the merits of any particular skin effects model. The method can easily accommodate other effects, which induces frequency dependence in the transmission line parameters. In time‐domain modeling, the parameters are assumed constant, and these models prove inefficient when incorporating these parameters as function of frequency. Therefore, a frequency‐domain simulation is implemented using harmonic analysis. Proper generalized decomposition (PGD) presents a separated representation and provides a quick and accurate solution for such problems.
Effects of colchicine on gingival inflammation, apoptosis, and alveolar bone loss in experimental periodontitis
Authors: Aral Cüneyt Asım , Aral Kübra , Yay Arzu , Özçoban Özge , Berdeli Afig , Saraymen Recep 
Published: February 2018

AbstractBackgroundThe aim of the study was to investigate the effects of colchicine on cytokine production, apoptosis, alveolar bone loss, and oxidative stress in an experimental model of periodontitis in rats.MethodsForty‐eight rats were divided equally into four groups: healthy (H); periodontitis (P); periodontitis+colchicine low dose (CL, 30 μg/kg/day), and periodontitis+colchicine high dose (CH, 100 μg/kg/day). After 11 days, interleukin (IL) ‐1β, IL‐8, and IL‐10 were analyzed in gingival samples using Enzyme‐Linked ImmunoSorbent Assay. Receptor activator of nuclear factor kappa‐B ligand (RANKL), osteoprotegerin (OPG), total oxidative stress (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured in gingiva and serum. Alveolar bone volume was evaluated via micro‐CT. Apoptotic cells were detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay in histological sections.ResultsColchicine treatment significantly reduced IL‐1β, IL‐8, RANKL, RANKL/OPG, TOS, OSI, and bone volume ratio levels, and increased TAS levels compared to group P (p < 0.05). High dose colchicine treatment (CH) significantly decreased TUNEL+ cell counts compared to group P (p < 0.05).ConclusionsThese finding suggest that colchicine has a prophylactic potential for the prevention of periodontal tissue destruction through anti‐inflammatory, anti‐oxidative, anti‐apoptotic, and bone‐protective effects.
Benthic uptake of phytoplankton and ocean‐reef exchange of particulate nutrients on a tide‐dominated reef
Authors: Gruber Renee K. , Lowe Ryan J. , Falter James L. 
Published: February 2018

AbstractBenthic fluxes of chlorophyll a (Chl a) and particulate organic carbon (POC) and nitrogen (PON) were quantified on Tallon reef, a strongly tide‐dominated (spring range > 8 m) reef located in the Kimberley region of northwestern Australia, over a 2‐week period. Extensive hydrodynamic observations were used to construct a reef‐scale mass balance to estimate material exchange between the reef and ocean over individual tidal cycles. Additionally, a one‐dimensional control volume approach was used to estimate fluxes of Chl a in waters traversing the reef platform. Particulate material was delivered to the reef platform in a pulse during flood tide, and benthic uptake of Chl a declined to negligible values toward the end of ebb tide. On the scale of tidal cycles, a net uptake of Chl a was observed on the reef platform (on average 1.3 mg Chl a m−2 d−1), which was lower than previous studies of many reef communities. Fluxes showed variability depending on the magnitude of individual tidal cycles, which was likely related to volumes of oceanic Chl a inputs. Tallon reef was a net source of detrital POC and PON to the surrounding coastal ocean, with average POC exports ∼ 3% of the reef's benthic gross primary production. Seasonal measurements of water quality reported here are among the first records for the coastal Kimberley, and suggest that reefs in the west Kimberley may experience naturally elevated levels of phytoplankton and particulate nutrients, especially during the wet season.
Correlation of injection moulding parameters with mechanical and morphological aspects of natural fibre reinforced thermoplastics
Authors: Saleh M.A. , Taha I. , Ebeid S.J. 
Published: February 2018

AbstractThe mechanical behaviour of fibre reinforced composites depends – amongst other things – on the morphological structure. This in turn is greatly affected by the processing conditions. In this study a 10 weight % flax fibre reinforced high density polyethylene composite is prepared by extrusion compounding followed by injection moulding. The effect of injection temperature, speed and rotational screw speed on the morphology of fibres and matrix are studied and related to the tensile and impact behaviour of the composites. Results indicate that fibre length is easily affected by all parameters under consideration. Whereas a higher injection temperature allows the preservation of fibre length, an excessive increase in temperature, however, leads to their thermal degradation. Increased injection and rotational speeds further introduce excessive shear stresses on the fibre, causing their breakage, similarly leading to reduced mechanical performance. Matrix structure is mainly affected by the injection temperature, where its increase leads to the reduction in the degree of crystallinity and thus also a decrease in tensile strength. Other processing parameters prove to have minor effect on matrix morphology.
Urinary Metabolomics Profiles Associated to Bovine Meat Ingestion in Humans
Authors: Khodorova Nadezda V. , Rutledge Douglas N. , Oberli Marion , Mathiron David , Marcelo Paulo , Benamouzig Robert , Tomé Daniel , Gaudichon Claire , Pilard Serge 
Published: February 2018

ScopeThe impact of meat consumption on human health is widely examined in nutritional epidemiological studies, especially due to the connection between the consumption of red and processed meat and the risk of colon cancer. Food questionnaires do not assess the exposure to different methods of meat cooking. This study aimed to identify biomarkers of the acute ingestion of bovine meat cooked with two different processes.Methods and resultsNon‐targeted UPLC‐MS metabolite profiling was done on urine samples obtained from 24 healthy volunteers before and 8 h after the ingestion of a single meal composed of intrinsically 15N labelled bovine meat, either cooked at 55 °C for 5 min or at 90 °C for 30 min. A discriminant analysis extension of independent components analysis was applied to the mass spectral data. After meat ingestion, the urinary excretion of 1‐methylhistidine, phenylacetylglutamine, and short‐ and medium‐chained acylcarnitines was observed. 15N labelling was detected in these metabolites, thus confirming their origin from ingested meat. However, no difference was observed in urinary metabolomic profiles according to the meat cooking process used.ConclusionMeat ingestion led to the excretion of several nitrogen‐containing compounds, but although a metabolic signature was detected for meat ingestion, the impact of the cooking process was not detectable at the level of urinary metabolic signature in our experimental conditions.
A mixed methods study of Tai Chi exercise for patients with chronic heart failure aged 70 years and older
Authors: Hägglund Lena , Boman Kurt , Brännström Margareta 
Published: February 2018

AbstractAims and objectivesThis study aimed to evaluate Tai Chi group training among patients with chronic heart failure (CHF) aged 70 years and older.BackgroundPhysical activity is recommended for CHF treatment. Tai Chi is found to be beneficial to different patient groups, although few studies focus on older patients with CHF.DesignA mixed methods study. Participants were randomly assigned to Tai Chi training twice a week for 16 weeks (N = 25) or control (N = 20). Quantitative data were collected at baseline, at the end of the training period and 6 months after training, assessing self‐rated fatigue and quality of life, natriuretic peptides and physical performance. Individual qualitative interviews were conducted with participants (N = 10) in the Tai Chi training group.ResultsNo statistical differences between the Tai Chi training group and the control group in quality of life or natriuretic peptides was found. After 16 weeks, the training group tended to rate more reduced activity and the control group rated more mental fatigue. Participants in the training group rated increased general fatigue at follow‐up compared with baseline. Qualitative interviews showed that Tai Chi training was experienced as a new, feasible and meaningful activity. The importance of the leader and the group was emphasized. Improvements in balance were mentioned and there was no physical discomfort.ConclusionTai Chi was experienced as a feasible and meaningful form of physical exercise for patients with CHF aged over 70 years despite lack of achieved health improvement. Further investigations, using feasibility and meaningfulness as outcome variables seems to be useful.
Course of maternal fatigue and its associated factors during the first 6 months postpartum: a prospective cohort study
Authors: Iwata Hiroko , Mori Emi , Sakajo Akiko , Aoki Kyoko , Maehara Kunie , Tamakoshi Koji 
Published: February 2018

AbstractAimsTo identify the course of maternal fatigue during the first 6 months postpartum and to determine factors associated with it.DesignA prospective cohort study.MethodsWomen (N = 2,697) in 13 Japanese hospitals provided longitudinal data using self‐report questionnaires at five time points. Maternal fatigue was assessed using the Postnatal Accumulated Fatigue Scale. We focused on the effect of maternal age and parity on the course of maternal fatigue and used a mixed between/within‐subjects analysis of variance. Factors associated with maternal fatigue were analysed using stepwise multiple regression.ResultsIn the 6‐month postpartum period, the level of fatigue was highest at 1 month and significantly decreased from 1–4 months postpartum. Primiparas showed a significantly higher level of fatigue than multiparas during hospital stay and their levels of fatigue more closely approximated the 1‐month peak. Multiparas showed significantly higher levels of fatigue than younger primiparas at 6‐month postpartum. Factors associated with maternal fatigue included satisfaction with sleep, concerns about child‐rearing, satisfaction with social support, financial burden and meal times per day.
Proteomic Studies on the Swim Bladder of the European Eel (Anguilla anguilla)
Authors: Sialana Fernando J. , Schneebauer Gabriel , Paunkov Ana , Pelster Bernd , Lubec Gert 
Published: February 2018

AbstractThe swim bladder of a fish is a vital organ that with gas gland cells in the swim bladder wall enables key physiological functions including buoyancy regulation in the face of different hydrostatic pressures. Specific gas gland cells produce and secrete acidic metabolites into the blood in order to reduce the physical solubility of gases and blood gas transport capacity for regulating the volume of the swim bladder. Transcriptomic analyses have provided evidence at the RNA level but no specific studies at the protein level have been carried out so far. Herein, it was the aim of the study to show swim bladder proteins of the yellow stage European eel by label‐free LCMS (Q‐Exactive Plus) that resulted in the identification of 6223 protein groups. Neurotransmitter receptors and transporters were enriched in the membrane fraction and enzymes for acid production were observed. The list of identified proteins may represent a useful tool for further proteomics experiments on this organ. All MS proteomics data are available at the PRIDE repository with the dataset identifier PXD007850.
Bilateral pulmonary thrombosis in a newborn with congenital nephrotic syndrome
Authors: Dufau Julie , Roué Jean‐Michel , Rioualen Stéphane 
Published: February 2018

AbstractIn the neonatal period, pulmonary thromboembolic episodes are rare and unknown events. Case of a preterm newborn who presented a bilateral thrombosis of the pulmonary arteries associated with a congenital nephrotic syndrome. Pediatricians should consider pulmonary thromboembolic episodes in cases of newborns with unexplained severe and hypoxic respiratory distress syndrome.
Anxiolytic and antidepressant activities of Pelargonium roseum essential oil on Swiss albino mice: Possible involvement of serotonergic transmission
Authors: Abouhosseini Tabari Mohaddeseh , Hajizadeh Moghaddam Akbar , Maggi Filippo , Benelli Giovanni 
Published: February 2018

The anxiolytic and antidepressant activities of the Reunion Geranium (Pelargonium roseum Willd) essential oil (EO) were evaluated in male Swiss albino mice by intraperitoneal administration of 10, 20, and 50 mg/kg bw using elevated plus maze (EPM), open‐field test (OFT), and forced swimming test (FST). Moreover, we evaluated whether the 5‐HT1A and GABAA–benzodiazepine receptor systems are involved in the anxiolytic effects through the coadministration of WAY‐100635 (a selective 5‐HT1A receptor antagonist) and flumazenil (an antagonist of benzodiazepine). GC–MS revealed the monoterpene alcohols citronellol (35.9%) and geraniol (18.5%) as the main components of the P. roseum EO. EO was effective in increasing the total number of entries and time spent in the open arms of EPM whereas number of rearing in OFT was significantly decreased in comparison with the control. In the FST, immobility time decreased in EO treated mice. Pretreatment with WAY‐100635, but not Flumazenil, was able to reverse the effects of the EO in the EPM and FST, indicating that the EO activity occurs via the serotonergic but not GABAergic transmission. Overall, results of this work showed significant anxiolytic and antidepressant activity of P. roseum EO and confirmed the traditional uses of Pelargonium species as calming agents.
Neuroprotective effects of 20(S)‐protopanaxatriol (PPT) on scopolamine‐induced cognitive deficits in mice
Authors: Lu Cong , Lv Jingwei , Dong Liming , Jiang Ning , Wang Yan , Wang Qiong , Li Yinghui , Chen Shanguang , Fan Bei , Wang Fengzhong , Liu Xinmin 
Published: February 2018

20(S)‐protopanaxatriol (PPT), one of the ginsenosides from Panax ginseng, has been reported to have neuroprotective effects and to improve memory. The present study was designed to investigate the protective effect of PPT on scopolamine‐induced cognitive deficits in mice. Male Institute of Cancer Research mice were pretreated with 2 different doses of PPT (20 and 40 μmol/kg) for 27 days by intraperitoneal injection, and scopolamine (0.75 mg/kg) was injected intraperitoneally for 9 days to induce memory impairment. Thirty minutes after the last pretreatment, the locomotor activity was firstly examined to evaluate the motor function of mice. Then, memory‐related behaviors were evaluated, and the related mechanism was further researched. It was founded that PPT treatment significantly reversed scopolamine‐induced cognitive impairment in the object location recognition experiment, the Morris water maze test, and the passive avoidance task, showing memory‐improving effects. PPT also significantly improved cholinergic system reactivity and suppressed oxidative stress, indicated by inhibition of acetylcholinesterase activity, elevation of acetylcholine levels, increasing superoxide dismutase activity and lowering levels of malondialdehyde in the hippocampus. In addition, the expression levels of Egr‐1, c‐Jun, and cAMP responsive element binding in the hippocampus were significantly elevated by PPT administration. These results suggest that PPT may be a potential drug candidate for the treatment of cognitive deficit in Alzheimer's disease.
PEG‐Functionalized Chitosan: A Biological Macromolecule as a Novel Corrosion Inhibitor
Authors: Srivastava Vandana , Chauhan Dheeraj S. , Joshi Prathamesh G. , Maruthapandian Viruthasalam , Sorour Ahmad A. , Quraishi Mumtaz A. 
Published: February 2018

AbstractIn the present work, we have synthesized chemically functionalized chitosan via cross‐linking with polyethylene glycol (PEG) and used it as a novel green corrosion inhibitor. This is the first study reporting the use of chitosan‐PEG (CS‐PEG) as corrosion inhibitor for mild steel in 1 M HCl. The study was performed using weight loss method, electrochemical measurements, surface morphology (AFM) and quantum chemical investigation. The maximum corrosion inhibition efficiency of 93.9% was obtained at a concentration of 200 mgL−1. The inhibitor was found to obey Langmuir adsorption isotherm and exhibited both physical and chemical adsorption. Electrochemical impedance spectroscopy (EIS) showed an increase in the polarization resistance which supported the adsorption and inhibition behavior. Tafel data showed a mixed type behavior with cathodic predominance. The data of quantum chemical calculations supported the experimental findings.
Modified Gold Nanoparticles for the Temperature‐Dependent Colorimetric Detection of Mercury and Methylmercury
Authors: Aulsebrook Margaret L. , Watkins Ethan , Grace Michael R. , Graham Bim , Tuck Kellie L. 
Published: February 2018

AbstractA method for temperature‐dependent sensing of both inorganic mercury (Hg2+) and organic methyl mercury (MeHg+) with the same gold nanoparticle (AuNP) system was achieved. The AuNPs were functionalised with a thymine moiety and aggregation was achieved through binding of a single mercuric species between two thymine molecules on the surface of the nanoparticles. Both Hg2+ and MeHg+ caused a significant change in the visible absorbance spectrum, resulting in a colour change from red to blue. Significantly, the two mercuric species produced different hues of blue, distinguishable by eye. This method could lead to the development of a method for tandem detection of Hg2+ and MeHg+ in environmental samples.