bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Solidago×niederederi Origin Based on the Accumulation of Phenolic Compounds in Plant Raw Materials
Authors: Radušienė Jolita , Marksa Mindaugas , Karpavičienė Birutė 
Published: March 2018

AbstractThis study provides the first phytochemical characterization of the morphologically identified natural hybrid Solidago×niederederi Khek compared with the native Solidago virgaurea and two invasive species, Canada goldenrod (Solidago canadensis L.) and giant goldenrod (Solidago gigantea Aiton). The phenolic compounds, namely, chlorogenic acid, rutin, isoquercitrin, hyperoside, and quercitrin, were detected in leaves and inflorescences by the high-performance liquid chromatography–photodiode array detector/ultraviolet (PAD/UV) method. All analyzed Solidago species contained all of the phenolic compounds investigated. The quantitative phytochemical differentiation among Solidago taxa was shown by principal component analysis. The results indicated that S. gigantea plants were characterized by significantly different quantities of phenolic compounds compared with three other Solidago taxa, which formed a separate cluster in the space of the principal component model, indicating the high similarity of their profiles. An additional multivariate analysis of the three species studied revealed a chemical gradient from S. canadensis to S. virgaurea with a slightly overlapping zone on the score plots presented by S.×niederederi and S. virgaurea accessions. The results showed that S.×niederederi was closely related to S. virgaurea. This result is suggestive of a hybrid origin with significant contributions from the native species. However, S.×niederederi was significantly different from its parental species with respect to chlorogenic acid and quercitrin in leaves and rutin with isoquercitrin in inflorescences. Conversely, samples indicating intermediate chemical composition between native S. virgaurea and invasive S. gigantea were not distinguished. The comparison of phenolic compound accumulation in Solidago plants supported the additional identification of the origin of S.×niederederi.
PARK2 inhibits osteosarcoma cell growth through the JAK2/STAT3/VEGF signaling pathway
Authors: Lei Zhong , Duan Huijie , Zhao Tengfei , Zhang Yuxiang , Li Guoqi , Meng Jiahong , Zhang Suzhan , Yan Weiqi 
Published: March 2018

AbstractOsteosarcoma (OS) is the most common primary malignant bone tumor mainly occurring in children and adolescents. In past decades, studies revealed that PARK2 was a vital tumor suppressor gene in many malignant solid tumors. However, the role of PARK2 in OS remains largely unclear. Therefore, we assessed PARK2 expression in OS tissue and adjacent non-tumor tissues by immunohistochemical (IHC) analysis, and evaluated PARK2 mRNA expression in OS cell lines by real-time PCR analysis. The HOS and U2OS cell lines were employed to establish a PARK2 overexpression model. Using this model, we investigated the potential role of PARK2 in OS and explored the underlying molecular mechanisms. Our study showed PARK2 was downregulated in OS tissue and cell lines, which was significantly associated with higher tumor stage (P < 0.05). Overexpression of PARK2 arrested the cell cycle, inhibited cell proliferation, migration, and invasion, induced cell apoptosis, and reduced tube formation in vitro. Moreover, overexpression of PARK2 significantly suppressed tumor growth and angiogenesis in vivo. Additionally, PARK2 negatively regulated OS development through the JAK2/STAT3/VEGF pathway. Our findings demonstrate that PARK2 is a tumor suppressor gene that may negatively affect OS growth and angiogenesis via partly inhibiting the JAK2/STAT3/VEGF signaling pathway.
Uniaxial strain control of spin-polarization in multicomponent nematic order of BaFe2As2
Authors: Kissikov T. , Sarkar R. , Lawson M. , Bush B. T. , Timmons E. I. , Tanatar M. A. , Prozorov R. , Bud’ko S. L. , Canfield P. C. , Fernandes R. M. , Curro N. J. 
Published: March 2018

AbstractThe iron-based high temperature superconductors exhibit a rich phase diagram reflecting a complex interplay between spin, lattice, and orbital degrees of freedom. The nematic state observed in these compounds epitomizes this complexity, by entangling a real-space anisotropy in the spin fluctuation spectrum with ferro-orbital order and an orthorhombic lattice distortion. A subtle and less-explored facet of the interplay between these degrees of freedom arises from the sizable spin-orbit coupling present in these systems, which translates anisotropies in real space into anisotropies in spin space. We present nuclear magnetic resonance studies, which reveal that the magnetic fluctuation spectrum in the paramagnetic phase of BaFe2As2 acquires an anisotropic response in spin-space upon application of a tetragonal symmetry-breaking strain field. Our results unveil an internal spin structure of the nematic order parameter, indicating that electronic nematic materials may offer a route to magneto-mechanical control.
Hydrogen peroxide positively regulates brassinosteroid signaling through oxidation of the BRASSINAZOLE-RESISTANT1 transcription factor
Authors: Tian Yanchen , Fan Min , Qin Zhaoxia , Lv Hongjun , Wang Minmin , Zhang Zhe , Zhou Wenying , Zhao Na , Li Xiaohui , Han Chao , Ding Zhaojun , Wang Wenfei , Wang Zhi-Yong , Bai Ming-Yi 
Published: March 2018

AbstractHydrogen peroxide (H2O2) is an important signaling molecule in plant developmental processes and stress responses. However, whether H2O2-mediated signaling crosstalks with plant hormone signaling is largely unclear. Here, we show that H2O2 induces the oxidation of the BRASSINAZOLE-RESISTANT1 (BZR1) transcription factor, which functions as a master regulator of brassinosteroid (BR) signaling. Oxidative modification enhances BZR1 transcriptional activity by promoting its interaction with key regulators in the auxin-signaling and light-signaling pathways, including AUXIN RESPONSE FACTOR6 (ARF6) and PHYTOCHROME INTERACTING FACTOR4 (PIF4). Genome-wide analysis shows that H2O2-dependent regulation of BZR1 activity plays a major role in modifying gene expression related to several BR-mediated biological processes. Furthermore, we show that the thioredoxin TRXh5 can interact with BZR1 and catalyzes its reduction. We conclude that reversible oxidation of BZR1 connects H2O2-mediated and thioredoxin-mediated redox signaling to BR signaling to regulate plant development.
GATA3 zinc finger 2 mutations reprogram the breast cancer transcriptional network
Authors: Takaku Motoki , Grimm Sara A. , Roberts John D. , Chrysovergis Kaliopi , Bennett Brian D. , Myers Page , Perera Lalith , Tucker Charles J. , Perou Charles M. , Wade Paul A. 
Published: March 2018

AbstractGATA3 is frequently mutated in breast cancer; these mutations are widely presumed to be loss-of function despite a dearth of information regarding their effect on disease course or their mechanistic impact on the breast cancer transcriptional network. Here, we address molecular and clinical features associated with GATA3 mutations. A novel classification scheme defines distinct clinical features for patients bearing breast tumors with mutations in the second GATA3 zinc-finger (ZnFn2). An engineered ZnFn2 mutant cell line by CRISPR–Cas9 reveals that mutation of one allele of the GATA3 second zinc finger (ZnFn2) leads to loss of binding and decreased expression at a subset of genes, including Progesterone Receptor. At other loci, associated with epithelial to mesenchymal transition, gain of binding correlates with increased gene expression. These results demonstrate that not all GATA3 mutations are equivalent and that ZnFn2 mutations impact breast cancer through gain and loss-of function.
Theory of isolated magnetic skyrmions: From fundamentals to room temperature applications
Authors: Büttner Felix , Lemesh Ivan , Beach Geoffrey S. D. 
Published: March 2018

AbstractMagnetic skyrmions are topological quasiparticles of great interest for data storage applications because of their small size, high stability, and ease of manipulation via electric current. However, although models exist for some limiting cases, there is no universal theory capable of accurately describing the structure and energetics of all skyrmions. The main barrier is the complexity of non-local stray field interactions, which are usually included through crude approximations. Here we present an accurate analytical framework to treat isolated skyrmions in any material, assuming only a circularly-symmetric 360° domain wall profile and a homogeneous magnetization profile in the out-of-plane direction. We establish the first rigorous criteria to distinguish stray field from DMI skyrmions, resolving a major dispute in the community. We discover new phases, such as bi-stability, a phenomenon unknown in magnetism so far. We predict materials for sub-10 nm zero field room temperature stable skyrmions suitable for applications. Finally, we derive analytical equations to describe current-driven dynamics, find a topological damping, and show how to engineer materials in which compact skyrmions can be driven at velocities >1000 m/s.
Statistically derived asymmetric membrane potentials from α-helical and β-barrel membrane proteins
Authors: Koehler Leman Julia , Bonneau Richard , Ulmschneider Martin B. 
Published: March 2018

AbstractModeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs). Various TFE scales have been derived to quantify the energy required or released to insert an amino acid or protein into the membrane. Experimental measurement of TFEs is challenging, and only few scales were extended to depth-dependent energetic profiles. Statistical approaches can be used to derive such potentials; however, this requires a sufficient number of MP structures. Furthermore, MPs are tightly coupled to bilayers that are heterogeneous in terms of lipid composition, asymmetry, and protein content between organisms and organelles. Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane. Our data confirm the ‘charge-outside’ and ‘positive-inside’ rules for β-barrels and α-helical proteins, respectively. We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet. Our data further suggest that pore-facing residues in β-barrels have a larger contribution to membrane insertion and stability than previously suggested.
Evaluating the potential of chelation therapy to prevent and treat gadolinium deposition from MRI contrast agents
Authors: Rees Julian A. , Deblonde Gauthier J.-P. , An Dahlia D. , Ansoborlo Camille , Gauny Stacey S. , Abergel Rebecca J. 
Published: March 2018

AbstractSeveral MRI contrast agent clinical formulations are now known to leave deposits of the heavy metal gadolinium in the brain, bones, and other organs of patients. This persistent biological accumulation of gadolinium has been recently recognized as a deleterious outcome in patients administered Gd-based contrast agents (GBCAs) for MRI, prompting the European Medicines Agency to recommend discontinuing the use of over half of the GBCAs currently approved for clinical applications. To address this problem, we find that the orally-available metal decorporation agent 3,4,3-LI(1,2-HOPO) demonstrates superior efficacy at chelating and removing Gd from the body compared to diethylenetriaminepentaacetic acid, a ligand commonly used in the United States in the GBCA Gadopentetate (Magnevist). Using the radiotracer 153Gd to obtain precise biodistribution data, the results herein, supported by speciation simulations, suggest that the prophylactic or post-hoc therapeutic use of 3,4,3-LI(1,2-HOPO) may provide a means to mitigate Gd retention in patients requiring contrast-enhanced MRI.
Structural insights into positive and negative allosteric regulation of a G protein-coupled receptor through protein-lipid interactions
Authors: Bruzzese Agustín , Gil Carles , Dalton James A. R. , Giraldo Jesús 
Published: March 2018

AbstractLipids are becoming known as essential allosteric modulators of G protein-coupled receptor (GPCRs). However, how they exert their effects on GPCR conformation at the atomic level is still unclear. In light of recent experimental data, we have performed several long-timescale molecular dynamics (MD) simulations, totalling 24 μs, to rigorously map allosteric modulation and conformational changes in the β2 adrenergic receptor (β2AR) that occur as a result of interactions with three different phospholipids. In particular, we identify different sequential mechanisms behind receptor activation and deactivation, respectively, mediated by specific lipid interactions with key receptor regions. We show that net negatively charged lipids stabilize an active-like state of β2AR that is able to dock Gsα protein. Clustering of anionic lipids around the receptor with local distortion of membrane thickness is also apparent. On the other hand, net-neutral zwitterionic lipids inactivate the receptor, generating either fully inactive or intermediate states, with kinetics depending on lipid headgroup charge distribution and hydrophobicity. These chemical differences alter membrane thickness and density, which differentially destabilize the β2AR active state through lateral compression effects.
Global warming in the context of 2000 years of Australian alpine temperature and snow cover
Authors: McGowan Hamish , Callow John Nikolaus , Soderholm Joshua , McGrath Gavan , Campbell Micheline , Zhao Jian-xin 
Published: March 2018

AbstractAnnual resolution reconstructions of alpine temperatures are rare, particularly for the Southern Hemisphere, while no snow cover reconstructions exist. These records are essential to place in context the impact of anthropogenic global warming against historical major natural climate events such as the Roman Warm Period (RWP), Medieval Climate Anomaly (MCA) and Little Ice Age (LIA). Here we show for a marginal alpine region of Australia using a carbon isotope speleothem reconstruction, warming over the past five decades has experienced equivalent magnitude of temperature change and snow cover decline to the RWP and MCA. The current rate of warming is unmatched for the past 2000 years and seasonal snow cover is at a minimum. On scales of several decades, mean maximum temperatures have undergone considerable change ≈ ± 0.8 °C highlighting local scale susceptibility to rapid temperature change, evidence of which is often masked in regional to hemisphere scale temperature reconstructions.
High-throughput fecundity measurements in Drosophila
Authors: Nouhaud Pierre , Mallard François , Poupardin Rodolphe , Barghi Neda , Schlötterer Christian 
Published: March 2018

AbstractFecundity is probably the most frequently studied fitness component in Drosophila. Nevertheless, currently used methods to measure fecundity are not well-suited for large-scale experiments, with many populations being assayed in parallel. Here we present a standardized pipeline to measure fecundity in many Drosophila population samples with substantially reduced hand on times. Using a high-contrast medium for egg laying, we developed a Java plug-in for ImageJ to quantify the number of eggs by image processing. We show that our method is fast and provides reliable egg counts.
Photoinduced Topological Phase Transitions in Topological Magnon Insulators
Authors: Owerre S. A. 
Published: March 2018

AbstractTopological magnon insulators are the bosonic analogs of electronic topological insulators. They are manifested in magnetic materials with topologically nontrivial magnon bands as realized experimentally in a quasi-two-dimensional (quasi-2D) kagomé ferromagnet Cu(1–3, bdc), and they also possess protected magnon edge modes. These topological magnetic materials can transport heat as well as spin currents, hence they can be useful for spintronic applications. Moreover, as magnons are charge-neutral spin-1 bosonic quasiparticles with a magnetic dipole moment, topological magnon materials can also interact with electromagnetic fields through the Aharonov-Casher effect. In this report, we study photoinduced topological phase transitions in intrinsic topological magnon insulators in the kagomé ferromagnets. Using magnonic Floquet-Bloch theory, we show that by varying the light intensity, periodically driven intrinsic topological magnetic materials can be manipulated into different topological phases with different sign of the Berry curvatures and the thermal Hall conductivity. We further show that, under certain conditions, periodically driven gapped topological magnon insulators can also be tuned to synthetic gapless topological magnon semimetals with Dirac-Weyl magnon cones. We envision that this work will pave the way for interesting new potential practical applications in topological magnetic materials.
Feedback followed bias generation scheme for complementary passive mixer
Authors: Liao Youchun , Mei Niansong , Zhang Zhaofeng 
Published: March 2018

A complementary passive mixer with feedback followed gate bias generation scheme is proposed. The gate voltage of the switches follows the common‐mode voltage (VCM) of the signal paths, so that the proposed mixer achieves constant switching gate‐to‐source bias voltage and coherent performances while VCM changes over a certain range. Furthermore, a dc offset cancelation method based on the bias generation circuit is proposed. The dc component of the intermediate frequency is feedbacked to the bias generation input to form a cancelation path. A behavioural model is deduced to prove the effect of the offset cancelation and second intercept point (IIP2) improvement. The proposed mixer is implemented in a 0.13 μm CMOS process, and measurement results show that robust performance with the VCM range of 0.5–0.7 V and 16 dB IIP2 improvement are obtained.
Influence of direct‐connected inverter with one power switch open circuit fault on electromagnetic field and temperature field of permanent magnet synchronous motor
Authors: Li Weili , Li Lin , Gao Hanying , Li Dong , Zhang Xiaochen , Fan Yu 
Published: March 2018

For permanent magnetic motors, the faults in inverter may cause serious mechanical vibration, winding overheating, and thermal demagnetisation for the magnets. Thus, this study addresses the problem of the electromagnetic field and temperature distributions for permanent magnet synchronous motor (PMSM) under open circuit fault (OCF) in the upper switch of one phase. Firstly, taking a 12.5 kW 2000 r/min PMSM as an example, the 2D transient electromagnetic field‐circuit coupling calculation model is established. Then the flowing paths of the three‐phase currents in inverter are analysed before and after the OCF in the upper switch of one phase. Next, by using the finite‐element method, the current harmonics, the electromagnetic torque, the rotating speed, and the losses in PMSM are investigated. Simultaneously, direct current component and torque pulsation are also derived. Based on the 3D temperature field, the temperature distributions in different parts of PMSM also are comparatively studied before and after this fault. Moreover, the temperature of permanent magnets, which is the part most seriously affected by the temperature, are further analysed. Finally, calculation and experimental tests prove the accuracy of the theoretical analysis. The obtained conclusions may provide some references for the limit operation and effective diagnosis for inverter faults.
Muscular Strength is Independently Associated with Cystatin C: The KORA-Age Study
Authors: Volaklis Konstantinos , Thorand Barbara , Peters Anette , Halle Martin , Margot Heier , Amann Ute , Ladwig Karl , Schulz Holger , Koenig Wolfgang , Meisinger Christa 
Published: March 2018

AbstractThe purpose of this study was to investigate if there is a link between muscular strength (MS) and markers of chronic kidney disease (CKD) among older adults. The cross-sectional analysis based on 1041 men and women, aged 65–94 years, who participated in the KORA-Age study. Participants underwent an interview and extensive examinations including anthropometric measurements, diseases and drug intake registration, determination of health-related behaviors, collection of blood samples for measurements of cystatin C and maximal muscle strength evaluation. One-Way ANOVA revealed significant differences in both mean cystatin C (1.16±0.37 vs. 1.03±0.29 vs. 0.93±0.24 mg/L, p<0.001) and mean eGFRcysC (63.61±18.61 vs. 72.14±18.92 vs. 79.87±18.19 ml/min/1.73 m2, p<0.05) across thirds of maximal muscular strength (from lowest to highest). MS in the lowest third was significantly associated with increased odds of having elevated cystatin C (OR: 1.70, 95% CI: 1.01–2.85, p=0.043) after controlling for age, gender, fat mass, fat-free mass, alcohol intake, smoking status, number of regularly used medications, multimorbidity status, hs-CRP, telomere length and levels of physical activity. Lower levels of MS are independently associated with higher concentrations of cystatin C and lower eGFRcysC in older individuals. Increasing the levels of muscular strength may be useful to prevent the age-related CKD disease of older adults.
Richtig einschätzen
Authors: Rotzinger Kerstin 
Published: March 2018

Zusammenfassung
Triage in der pädiatrischen Notaufnahme Zwei Säuglinge kommen in die
Notaufnahme, einer apathisch, einer laut schreiend. Welcher soll zuerst versorgt
werden? Gerade bei Patienten, die sich verbal nicht zu ihren Schmerzen äußern
können, sind objektive Einschätzungsinstrumente wichtig. Triagesysteme für
Erwachsene gibt es schon seit mehr als 200 Jahren – für die Einschätzung von
Kindern gelten dabei allerdings spezielle Regeln.
Carnosine in health and disease
Authors: Artioli Guilherme Giannini , Sale Craig , Jones Rebecca Louise 
Published: March 2018

AbstractCarnosine was originally discovered in skeletal muscle, where it exists in larger amounts than in other tissues. The majority of research into the physiological roles of carnosine have been conducted on skeletal muscle. Given this and the potential for muscle carnosine content to be increased with supplementation, there is now a large body of research examining the ergogenic effects (or otherwise) of carnosine. More recent research, however, points towards a potential for carnosine to exert a wider range of physiological effects in other tissues, including the brain, heart, pancreas, kidney and cancer cells. Taken together, this is suggestive of a potential for carnosine to have therapeutic benefits in health and disease, although this is by no means without complication. Herein, we will provide a review of the current literature relating to the potential therapeutic effects of carnosine in health and disease.