bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative outcomes of adenosquamous carcinoma of the pancreas: An analysis of the National Cancer Database
Authors: Hester Caitlin A. , Augustine Mathew M. , Choti Michael A. , Mansour John C. , Minter Rebecca M. , Polanco Patricio M. , Porembka Matthew R. , Wang Sam C. , Yopp Adam C. 
Published: June 2018

BackgroundA paucity of data exists regarding the natural history and outcome measures of adenosquamous carcinoma of the pancreas (ASCP), a histology distinct from pancreatic adenocarcinoma (PDAC). The aim of this study is to characterize the clinicopathological features of ASCP in a large cohort of patients comparing outcome measures of surgically resected patients to PDAC.MethodsWe identified patients diagnosed with ASCP or PDAC from the National Cancer Database from 2004 to 2012. Patient demographics, tumor characteristics, treatment regimens, and overall survival were analyzed between the groups.ResultsWe identified 207 073 patients: 205 328 (99%) in the PDAC group and 1745 (1%) in the ASCP group. ASCP tumors were larger, located more frequently in a body/tail location (36% vs 24%, P < 0.001), undifferentiated/anaplastic histology (41% vs 17%, P < 0.001), and early stage presentation, (39% vs 32%, P < 0.001). There was no significant difference in OS when comparing all patients with PDAC and ASCP (6.2 months and 5.7 months, P = 0.601). In surgical patients ASCP histology was associated with worse OS (14.8 months vs 20.5 months, P < 0.001) but had lower nodal involvement (55% vs 61%, P < 0.001). ASCP histology was independently associated with worse OS, after adjusting for tumor characteristics, treatment, and patient demographics. In patients with only resected ASCP histology, negative lymph node status, R0 surgical resection, and receipt of chemotherapy was independently associated with improved overall survival following surgical resection.ConclusionAlthough patients with ASCP and PDAC tumors have similar survival when non‐surgical and surgical patients are combined, ASCP is associated with worse survival in stage I/II resected patients.
Silica‐based magnetic hybrid nanocomposite for the extraction and preconcentration of some organophosphorus pesticides before gas chromatography
Authors: Malek Somayeh Koupaei , Nodeh Hamid Rashidi , Akbari‐Adergani Behrouz 
Published: June 2018

AbstractThe precise control of pesticide residues in foodstuffs depends significantly on the clean extraction of analytes using specifically designed separation methods. In this study, a one‐pot sol–gel process was used for the preparation of a magnetic hybrid silica gel tetraethylortho silicate‐cyanopropyltriethoxy silane nanocomposite. The prepared material was characterized using energy dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy, single‐point specific surface area, and scanning electron microcopy. The synthesized magnetic hybrid material was used as a solid phase extraction sorbent for the extraction and preconcentration of some organophosphorus pesticides before gas chromatography with a microelectron capture detector. The performance of the proposed magnetic solid‐phase extraction technique was validated by linearity (0.05–2 ng/mL), correlation coefficients (r2 = 0.9993–0.9997), limit of detection (0.02–0.06 ng/mL, S/N = 3, n = 3), and intraday (RSD = 1.5–8.7%, n = 3) and interday precision (RSD = 5.5–9.3%, n = 12), while the recovery in real samples and equilibrium adsorption capacity was 72.02–103.84% and 8–20 mg/g, respectively. The magnetic solid‐phase extraction based on the hybrid nanocomposite revealed a high enrichment factor, an appropriate dynamic range, and great absorptive ability toward the selected organophosphorus pesticides spiked in real water samples.
Preparation and evaluation of temperature and magnetic dual‐responsive molecularly imprinted polymers for the specific enrichment of formononetin
Authors: Zhang Yu‐Zhen , Zhang Jiawei , Tan Ling , Xia Zhining , Wang Chong‐Zhi , Zhou Lian‐Di , Zhang Qihui , Yuan Chun‐Su 
Published: June 2018

AbstractThermo‐responsive magnetic molecularly imprinted polymers were prepared by simple surface molecular imprinting polymerization for the selective adsorption and enrichment of formononetin from Trifolium pretense by temperature regulation. Using formononetin as a template, N‐isopropylacrylamide as the thermo‐responsive functional monomer, and methacrylic acid as an assisting functional monomer, the polymers were synthesized on the surface of the magnetic substrate. The results show that imprinted polymers attained controlled adsorption of formononetin in response to the temperature change, with large adsorption capacity (16.43 mg/g), fast kinetics (60 min) and good selectivity at 35°C compared with that at 25 and 45°C. The selectivity experiment indicated that the materials had excellent recognition ability for formononetin and the selectivity factors were between 1.32 and 2.98 towards genistein and daidzein. The excellent linearity was attained in the range of 5–100 μg/mL, with low detection limits and low quantitation limits of 0.017 and 0.063 μg/mL, respectively. Furthermore, the thermo‐responsive magnetic molecularly imprinted polymers were successfully utilized for enriching and purifying formononetin from Trifolium pretense. The analytical results indicate that the imprinted polymers are promising materials for selective identification and enrichment of formononetin in complicated herbal medicines by simple temperature‐responsive regulation.
Determination of major phenolic compounds in apples: Part I—Optimization of high‐performance liquid chromatography separation with diode array detection
Authors: Sklenářová Hana , Bílková Aneta , Pechová Martina , Chocholouš Petr 
Published: June 2018

AbstractThe separation of seven phenolic compounds including gallic acid, chlorogenic acid, epicatechin, quercitrin, rutin, phloridzin, and phloretin present in apple peel and pulp and differing in elution properties has been optimized using high‐performance liquid chromatography with diode array detection. Several stationary phases were tested to achieve the efficient separation of phenolic compounds in fruit extracts and C18 was found to be the most efficient. Core–shell and fully porous C18 packings were assessed with respect to the complex composition of the fruit extracts. The developed high‐performance liquid chromatography method comprised gradient elution in which mobile phase A was water at pH 2.8 adjusted with acetic acid and B was acetonitrile. The gradient shape was the following: 0 min 95% A/5% B, 2.5 min 85% A/15% B, 12 min 50% A/50% B, 15 min 95% A/5% B. The flow rate was 1 mL/min, injection volume 10 μL, and UV detection at 255, 280, 320, and 365 nm was applied. Our method was validated for both C18 core–shell and fully porous packings. The resolution 6.2–14.8, symmetry 0.99–1.34, peak capacity 18–60, peak area repeatability 0.45–1.00% relative standard deviation, calibration range 0.125–5 mg/mL (0.25–10 mg/mL for chlorogenic acid and rutin), correlation coefficients of calibration curve 0.9976–0.9997, and accuracy evaluated as recovery 95.56–107.54% were determined for the core–shell column.
Preparation and application of chlorpyrifos molecularly imprinted solid‐phase microextraction probes for the residual determination of organophosphorous pesticides in fresh and dry foods
Authors: Ma Jin‐Kui , Huang Xiao‐Chen , Wei Shou‐Lian 
Published: June 2018

AbstractA solid‐phase microextraction probe was prepared on the surface of a stainless‐steel wire through molecular sol–gel imprinting technology using chlorpyrifos as a template molecule, tetraethoxysilane as a sol–gel precursor, and acrylamide and β‐cyclodextrin as functional monomers. The polymer was characterized by infrared spectrometry and scanning electron microscopy. Moreover, the selectivity and the parameters including the type and volume of the extraction solvents, ionic strength, pH, temperature, extraction time, stirring speed, and desorption time affecting extraction performance were evaluated. Under the optimum solid‐phase microextraction and gas chromatography conditions, the linear ranges were 0.25–25.0 μg/L for chlorpyrifos, quinalphos, triazophos, pirimiphos‐methyl, and chlorpyrifos‐methyl with the correlation coefficient above 0.99. The detection limits (S/N = 3) were in the range of 0.02–0.07 μg/L and the RSDs were <7.3%. The developed method was successfully used to determine the multi‐residues of chlorpyrifos, quinalphos, triazophos, pirimiphos‐methyl, and chlorpyrifos‐methyl in green peppers and cinnamon with satisfactory recoveries.
Out of the Mouths of Babes: Links Between Linguistic Structure of Loss Narratives and Psychosocial Functioning in Parentally Bereaved Children
Authors: Kaplow Julie B. , Wardecker Britney M. , Layne Christopher M. , Kross Ethan , Burnside Amanda , Edelstein Robin S. , Prossin Alan R. 
Published: June 2018

AbstractThis study examined links between the language bereaved children use to describe the death of their caregiver and children's psychological/behavioral functioning and coping strategies. Participants included 44 children (54.5% male) aged 7 to 12 (M = 9.05) years who were bereaved by the death of a caregiver. Children were assessed via self‐ and caregiver‐report measures and an in‐person interview regarding the loss of their caregiver. Children's loss narratives gathered through in‐person interviews were transcribed and subjected to textual analysis. Linguistic categories included pronouns and verb tense. Drawing from linguistic and self‐distancing theories, we hypothesized that children's use of language reflecting self‐distancing (third‐person pronouns and past tense) or social connectedness (first‐person plural pronouns) would be negatively associated with psychological/behavioral distress and avoidant coping. Similarly, we expected that children's use of self‐focused language (first‐person singular pronouns and present tense) would be positively associated with psychological/behavioral distress and avoidant coping. As hypothesized, preliminary findings suggest that children who employed more self‐distancing language and used more social connectedness words reported less avoidant coping, rs = .40–.42. Also as hypothesized, children who employed more self‐focused language had higher levels of self‐reported posttraumatic stress symptoms, r = .54, and avoidant coping, r = .54, and higher parent‐reported psychological/behavioral distress, r = .43. Implications for theory‐building, risk screening, and directions for future research with bereaved youth are discussed.
Ecology of neonate eastern box turtles with prescribed fire implications
Authors: Laarman Patrick B. , Keenlance Paul W. , Altobelli Joseph T. , Schumacher Chris M. , Huber Phillip , Jacquot Joseph J. , Moore Jennifer A. 
Published: June 2018

ABSTRACTLittle is known about the movements and behavior of neonate eastern box turtles (Terrapene carolina carolina). We investigated spatial ecology of neonate eastern box turtles at 4 upland openings in the Manistee National Forest, Michigan, USA, 2012–2016. We protected nests and used radio‐telemetry to document dispersal from nests, land cover types used for overwintering, and residency time of neonates in natal openings. We used binomial logistic regression to model probabilities of overwintering in natal openings versus adjacent forest and edge. We protected 64 nests, radio‐fitted 64 neonates over 4 cohort years, tracked 46 neonates from nest emergence to their overwintering sites, and tracked 28 neonates for portions of their second activity season. Dispersal (straight‐line distance from nest to overwintering site) was limited in the first activity season (n = 46 neonates,  = 19.9 ± 2.4 m [SE], range = 1.9–83.2 m) and 24 overwintered in natal openings, 4 overwintered in the forest edge, and 18 overwintered in forest. Our best logistic model included the predictor variables distance from nest to nearest forest edge and day of nest emergence. Probability of overwintering within a natal opening was greater for neonates from nests located farther from the forest edge and for neonates emerging from nests later in the year. By 1 June of their second activity season, all tracked turtles were still present in, or within 50 m, of their natal opening, but all neonates had dispersed into surrounding forest or wetlands by 1 July. Managers should be aware that neonates reside in or very near their natal openings for several months after nest emergence. When burning or otherwise intensively managing open canopy box turtle nesting areas, relaxing the interval (yr) between treatments and excluding forest edge and adjacent forest should allow neonates time to disperse, decreasing risk of mortality. © 2018 The Wildlife Society.
Kinetics of the ene reactions of 4‐phenyl‐1,2,4‐triazoline‐3,5‐dione with β‐pinene and 2‐carene: Temperature, high pressure, and solvent effects
Authors: Kiselev Vladimir D. , Kornilov Dmitry A. , Anikin Oleg V. , Shulyatiev Alexey A. , Kolesnikova Anastasia O. , Konovalov Alexander I. 
Published: June 2018

AbstractThe data on temperature, solvent, and high hydrostatic pressure influence on the rate of the ene reactions of 4‐phenyl‐1,2,4‐triazoline‐3,5‐dione (1) with 2‐carene (2), and β‐pinene (4) have been obtained. Ene reactions 1+2 and 1+4 have high heat effects: ∆Hr‐n (1+2) −158.4, ∆Hr‐n(1+4) −159.2 kJ mol−1, 25°C, 1,2‐dichloroethane. The comparison of the activation volume (∆V≠(1+2) −29.9 cm3 mol−1, toluene; ∆V≠(1+4) −36.0 cm3 mol−1, ethyl acetate) and reaction volume values (∆Vr‐n(1+2) −24.0 cm3 mol−1, toluene; ∆Vr‐n(1+4) −30.4 cm3 mol−1, ethyl acetate) reveals more compact cyclic transition states in comparison with the acyclic reaction products 3 and 5. In the series of nine solvents, the reaction rate of 1+2 increases 260‐fold and 1+4 increases 200‐fold, respectively, but not due to the solvent polarity.
Titanium Oxide Sol–Gel Induced Wrinkling of Electrospun Nanofibers
Authors: Park Yaewon , Ford Ericka 
Published: June 2018

AbstractTiO2sol–gel reaction induces nanowrinkling along the surface of electrospun poly(vinyl alcohol) (PVA) nanofibers. Nanowrinkling along nanofibers is influenced by electrospinning voltage, degree of nanofiber crosslinking, and the repetitive immersion of nanofibers in TiO2precursors. Crosslinked nanofibers are dipped in alternating solutions of titanium tetraisopropoxide (TTIP) and water for up to five cycles. Interestingly, nanowrinkles only form along nanofibers that are spun at 45 kV and treated with three or more cycles of sol–gel precursor. Spectroscopy reveals that more PVA hydroxyl groups populate the nanofiber surface when electrospinning occurs at 45 kV than at 15 kV. In turn, surface PVA hydroxyl groups appear to nucleate TiO2growth. Scanning probe phase micrographs confirm modulus differences between the attached TiO2particles and PVA's surface. Those moduli differences result in wrinkling, as TTIP‐treated nanofibers undergo repetitive cycles of water swelling and isopropanol deswelling.
Metal‐Coupled Fluorescence Resonance Energy Transfer in Layer‐by‐Layer Assemblies for Dual Modality Fluorescence Enhancement
Authors: Kim Ki‐Se , Yoo Seong Il , Sohn Byeong‐Hyeok 
Published: June 2018

AbstractFluorescence resonance energy transfer (FRET) has attracted a great deal of attention in chemical and biological analysis because fluorescence intensity of given fluorophores (acceptor) can be amplified by placing energy‐harvesting molecules (donor) in their vicinity. However, FRET‐based fluorescence has a certain limitation in terms of signal amplification because it inherently relies on the intrinsic properties of donors and acceptors as well as the donor‐to‐acceptor distance. To overcome this limitation, metal nanoparticles (NPs) are introduced to a FRET system to engineer the dipole–dipole interaction in FRET using the localized surface plasmon resonance (LSPR) of metal NPs. A 63.1‐fold fluorescence enhancement is observed using an LSPR‐coupled FRET process based on layer‐by‐layer (LbL) assemblies composed of a pair of donor–acceptor fluorescent dyes and metal NPs. It is discovered that by a) optimizing LSPR‐coupled excitation enhancement in donors as well as emission enhancement in acceptors and b) opening FRET channels between LSPR‐coupled donors and acceptors, dual enhancement mechanisms based on FRET and LSPR can be coupled to induce strong fluorescence. Interestingly, it is noticed that the fluorescence enhancement is achieved with reduced FRET efficiency, which is explained by competitive near‐field interactions in the LSPR‐coupled FRET system.
Enhancement of methanogenesis by electric syntrophy with biogenic iron‐sulfide minerals
Authors: Kato Souichiro , Igarashi Kensuke 
Published: June 2018

AbstractRecent studies have shown that interspecies electron transfer between chemoheterotrophic bacteria and methanogenic archaea can be mediated by electric currents flowing through conductive iron oxides, a process termed electric syntrophy. In this study, we conducted enrichment experiments with methanogenic microbial communities from rice paddy soil in the presence of ferrihydrite and/or sulfate to determine whether electric syntrophy could be enabled by biogenic iron sulfides. Although supplementation with either ferrihydrite or sulfate alone suppressed methanogenesis, supplementation with both ferrihydrite and sulfate enhanced methanogenesis. In the presence of sulfate, ferrihydrite was transformed into black precipitates consisting mainly of poorly crystalline iron sulfides. Microbial community analysis revealed that a methanogenic archaeon and iron‐ and sulfate‐reducing bacteria (Methanosarcina, Geobacter, and Desulfotomaculum, respectively) predominated in the enrichment culture supplemented with both ferrihydrite and sulfate. Addition of an inhibitor specific for methanogenic archaea decreased the abundance of Geobacter, but not Desulfotomaculum, indicating that Geobacter acquired energy via syntrophic interaction with methanogenic archaea. Although electron acceptor compounds such as sulfate and iron oxides have been thought to suppress methanogenesis, this study revealed that coexistence of sulfate and iron oxide can promote methanogenesis by biomineralization of (semi)conductive iron sulfides that enable methanogenesis via electric syntrophy.
Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy
Authors: Berisha Bajram , Schams Dieter , Rodler Daniela , Sinowatz Fred , Pfaffl Michael W. 
Published: June 2018

The aim of this study was to characterize certain prostaglandin family members in the bovine corpus luteum (CL) during the estrous cycle and pregnancy. The CL tissue was assigned to the stages 1–2, 3–4, 5–7, 8–12, 13–16 and >18 days (after regression) of the estrous cycle and 1–2, 3–4, 6–7, and >8 months of pregnancy. In these samples, we investigated prostaglandin F2alpha (PTGF), prostaglandin E2 (PTGE), their receptors (PTGFR, PTGER2, and PTGER4), cyclooxygenase 2 (COX‐2), PTGF synthase (PTGFS), and PTGE synthase (PTGES). The expression of messenger RNA (mRNA) was measured by reverse transcription quantitative polymerase chain reaction, hormones by enzyme immunoassay, and localization by immunohistochemistry. The mRNA expression of COX‐2, PTGFS, and PTGES in CL during the early‐luteal phase was high followed by a continuous and significant downregulation afterward, as well as during all phases of pregnancy. The concentration of PTGF in CL tissue was high during the early‐luteal phase, decreased significantly in the mid‐luteal phase, and increased again afterward. In contrast, the concentration of PTGE increased significantly during the late‐luteal phase followed by a decrease during regression. The PTGE level increased again during late pregnancy. Immunohistochemically, the large granulose–luteal cells show strong staining for COX‐2 and PTGES during the early‐luteal stage followed by lower activity afterward. During pregnancy, most of the luteal cells were only weakly positive or negative. In conclusion, our results indicate that the examined prostaglandin family members are involved in the local mechanisms that regulate luteal function, specifically during CL formation, function, and regression and during pregnancy in the cow.
EnLIGHTenment of tumor vessel normalization and immunotherapy in glioblastoma
Authors: Treps Lucas 
Published: June 2018

AbstractGlioblastoma multiforme (GBM) is a highly vascularized and aggressive brain tumor. Despite aggressive standard care, GBM remains predominantly fatal; hence, new innovative therapies are required. Recent research published in the Journal of Pathology has identified the CGKRK peptide as a promising tool with which to specifically target the tumor vasculature from high‐grade glioma. This tumor vessel‐homing peptide was fused to the tumor necrosis factor superfamily member LIGHT/TNFSF14, and injected intravenously into murine orthotopic GBM models. After treatment, the tumor vasculature appeared to be less abnormal, with normalized features such as increased endothelial barrier integrity, pericyte contractility, and tumor perfusion. Moreover, CGKRK–LIGHT induced the appearance of high endothelial venules (HEVs), which are specialized structures that play a role in lymphocyte trafficking and have been shown to increase T‐cell infiltration in solid tumors. Combining CGKRK–LIGHT with anti‐angiogenic and immune checkpoint blockade treatments boosted HEV induction and cytotoxic T‐cell infiltration, leading to a reduction in tumor burden. In this Commentary, I highlight the therapeutic opportunities provided by and the current limitations of LIGHT‐vascular targeting peptide as a new approach to target GBM and enhance tumor vessel delivery and immunotherapy efficacy. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Activation of MET signaling by HDAC6 offers a rationale for a novel ricolinostat and crizotinib combinatorial therapeutic strategy in diffuse large B‐cell lymphoma
Authors: Liu Zebing , Cai Ying , Yang Yu , Li Anqi , Bi Rui , Wang Lisha , Shen Xiaohan , Wang Weige , Jia Yijun , Yu Baohua , Cao Bing , Cui Wenli , Wei Ping , Zhou Xiaoyan 
Published: June 2018

AbstractSome histone deacetylases (HDACs) promote tumor cell growth and pan‐ or selective HDAC inhibitors are active in some cancers; however, the pivotal HDAC enzyme and its functions in human diffuse large B‐cell lymphoma (DLBCL) remain largely unknown. Using NanoString nCounter assays, we profiled HDAC mRNA expression and identified HDAC6 as an upregulated HDAC family member in DLBCL tissue samples. We then found that HDAC6 plays an oncogenic role in DLBCL, as evidenced by its promotion of cell proliferation in vitro and tumor xenograft growth in vivo. Mechanistically, the interaction between HDAC6 and HR23B downregulated HR23B expression, thereby reducing the levels of casitas B‐lineage lymphoma (c‐Cbl), an E3 ubiquitin ligase for hepatocyte growth factor receptor (MET), which resulted in the inhibition of MET ubiquitination‐dependent degradation. In addition, enhanced HDAC6 expression and decreased HR23B expression were correlated with poor overall survival rates among patients with DLBCL. Taken together, these results establish an HDAC6–HR23B–MET axis and indicate that HDAC6 is a potent promoter of lymphomagenesis in DLBCL. Thus, a therapeutic strategy based on HDAC6 inhibitors in combination with MET inhibitors is promising. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.