bluuebunny/crossref_metadata_2025_split huggingface dataset:
LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21
Authors: Li Gang , Wang Changming , Wang Yongbing , Xu Bin , Zhang Wenzhong 
Published: August 2018

AbstractLong non‐coding RNAs (lncRNAs) have emerged as important regulators of cancer, including colorectal cancer (CRC). The exact expression pattern of long intergenic noncoding RNA 00312 (LINC00312) in CRC and its mechanisms of action have not been reported. Here, we found that LINC00312 is underexpressed in CRC tissues and cell lines. Functional experiments suggested that LINC00312 suppresses growth, migration and invasion of CRC cells in vitro and attenuates tumour proliferation and metastasis in vivo. Mechanistically, LINC00312 was found to regulate the malignancy of CRC cells by binding to miR‐21 and by functioning as a tumour suppressor targeting PTEN. Overexpression of miR‐21 or knockdown of PTEN attenuated the LINC00312‐mediated inhibition of CRC cell proliferation and invasion. Taken together, our results elucidate the role of the LINC00312–miR‐21–PTEN axis in CRC cell proliferation and tumour progression and may lead to new lncRNA‐based diagnostics or therapeutics for CRC.
Deletion of caveolin‐1 attenuates LPS/GalN‐induced acute liver injury in mice
Authors: Tsai Tsung‐Huang , Tam Kabik , Chen Shu‐Fen , Liou Jun‐Yang , Tsai Yi‐Chen , Lee Yen‐Ming , Huang Tai‐Yu , Shyue Song‐Kun 
Published: August 2018

AbstractAcute hepatic injury caused by inflammatory liver disease is associated with high mortality. This study examined the role of caveolin‐1 (Cav‐1) in lipopolysaccharide (LPS) and D‐galactosamine (GalN)‐induced fulminant hepatic injury in wild type and Cav‐1‐null (Cav‐1−/−) mice. Hepatic Cav‐1 expression was induced post‐LPS/GalN treatment in wild‐type mice. LPS/GalN‐treated Cav‐1−/− mice showed reduced lethality and markedly attenuated liver damage, neutrophil infiltration and hepatocyte apoptosis as compared to wild‐type mice. Cav‐1 deletion significantly reduced LPS/GalN‐induced caspase‐3, caspase‐8 and caspase‐9 activation and pro‐inflammatory cytokine and chemokine expression. Additionally, Cav‐1−/− mice showed suppressed expression of Toll‐like receptor 4 (TLR4) and CD14 in Kupffer cells and reduced expression of vascular cell adhesion molecule 1 and intercellular adhesion molecule 1 in liver cells. Cav‐1 deletion impeded LPS/GalN‐induced inducible nitric oxide synthase expression and nitric oxide production and hindered nuclear factor‐κB (NF‐κB) activation. Taken together, Cav‐1 regulated the expression of mediators that govern LPS‐induced inflammatory signalling in mouse liver. Thus, deletion of Cav‐1 suppressed the inflammatory response mediated by the LPS‐CD14‐TLR4‐NF‐κb pathway and alleviated acute liver injury in mice.
STAT3 promotes tumour progression in glioma by inducing FOXP1 transcription
Authors: Sun Xinlin , Wang Jihui , Huang Min , Chen Taoliang , Chen Jiansheng , Zhang Fabing , Zeng Huijun , Xu Zhimin , Ke Yiquan 
Published: August 2018

AbstractObjectiveThis paper investigated the effects of STAT3 through promoting FOXP1 transcription on proliferation, apoptosis and invasion in glioma cells.MethodsQuantitative real‐time PCR (qRT‐PCR) and Western blot assay were administered to assess the mRNA and protein expression levels of STAT3 and FOXP1 in glioma tissues and cells, respectively. Luciferase reporter and Chromatin Immunoprecipitation (ChIP) assays were implemented to determine the correlation between STAT3 and FOXP1. MTT and colony formation assays were conducted to identify cell growth. Flow cytometry was run to detect the cell apoptosis rate of glioma cells. Transwell assays were conducted to reveal cell invasion ability.ResultsThe mRNA and protein expression levels of STAT3 were highly expressed in glioma tissues and cells. After cells transfected with siRNA of STAT3, both STAT3 and FOXP1 were simultaneously downregulated. STAT3 directly regulated FOXP1 transcription. STAT3 promoted cell proliferation, inhibited cell apoptosis and enhanced cell invasion through promoting FOXP1 transcription in glioma cells.ConclusionIn summary, STAT3 gene was a transcriptional regulator of FOXP1. Depleted STAT3 restrained cell proliferation and invasion, promoted cell apoptosis in glioma cells. This molecular mechanism between STAT3 and FOXP1 can serve as a therapeutic target for glioma treatment.
Identification of pesticide residues in lettuce leaves based on near infrared transmission spectroscopy
Authors: Sun Jun , Ge Xiao , Wu Xiaohong , Dai Chunxia , Yang Ning 
Published: August 2018

AbstractTo explore the nondestructive and accurate qualitative detection method of pesticide residues in vegetables, lettuce leaves were taken as carrier and pesticides (fenvalerate and chlorpyrifos) were taken as research object. The near infrared spectral data of lettuce leaves was collected by using near infrared spectrometer NIRez (950–1,650 nm), then it was preprocessed by SNV algorithm and SG‐SNV combination, respectively. The successive projections algorithm (SPA), competitive adaptive reweighted sampling (CARS), iteratively retaining informative variables (IRIV), and combination of two of them were used to select characteristic wavelengths from the preprocessed spectral data, respectively. In this study, support vector machine (SVM) was used to classify pesticide residues in lettuce leaves. Because the classification performance of SVM was influenced by parameters c (penalty coefficient) and g (kernel function parameter), so the parameters c and g of SVM were optimized by gravitational search algorithm (GSA). Finally, identification models were built on the full wavelengths and the selected characteristic wavelengths, respectively. Final result showed that CARS‐IRIV‐GSA‐SVM model had the best performance, and the classification accuracy of training set and predication set reached 100 and 98.33%. Therefore, near infrared transmission spectroscopy can be applied to the qualitative detection of pesticide residues in lettuce leaves.Practical applicationsLettuce leaves can be eaten raw, but pesticide residues in lettuce leaves are likely to do harm to human health. Near infrared transmission spectroscopy, a fast and nondestructive technology, can be used to detect pesticide residues in lettuce leaves. Furthermore, the small NIR spectrometer used in this study can also provide some idea for the production of portable pesticide residue detector. This study demonstrated that near infrared transmission spectroscopy can be applied to the qualitative detection of pesticide residues in lettuce leaves.
Evaluating quality of tomato during storage using fusion information of computer vision and electronic nose
Authors: Huang Xing‐yi , Pan Si‐hui , Sun Zhao‐yan , Ye Wei‐tao , Aheto Joshua Harrington 
Published: August 2018

AbstractA nondestructive method for evaluation of tomato quality was proposed to recognize the different ripening stages of tomatoes and to predict their hardness. Computer vision and electronic nose were used, and data fusion technology was used to establish the decision model of multi‐attribute information fusion. Fisher discriminant analysis (FDA) model and support vector classifier (SVC) model for tomato grading were established. Partial least squares (PLS) model and support vector regression (SVR) model were used for predicting the hardness. Comparing with the single detection technique, the combined system which fused computer vision and electronic nose, achieved better results. In the combined system: with respect to the SVC model, classification accuracy was 96.38% for the training set, 94.20% for the prediction set; while to the FDA model, classification accuracy was 93.48% for the training set, 85.51% for the prediction set; to the PLS model, the correlation coefficient of prediction (Rp) was 94.09% and the root mean square error of prediction (RMSEP) was 2.33 N; to the SVR model, the Rp was 95.14% and RMSEP was 0.03 N. It was observed that more robust and better prediction performance on tomato detection were achieved by using fusion information.Practical applicationsComputer vision, electronic nose, data fusion, and pattern recognition were used for the evaluation of tomato ripeness and hardness. Computer vision captured the color information of tomatoes and electronic nose responded to the smell of tomatoes. After data fusion, information became more abundant and the discrimination models also got better performance. It proved the possibility of nondestructive method for the evaluation of tomatoes quality during storage. So it is feasible to apply this method to detect tomato quality in factory.
Relationships Influence Health: Family Theory in Health‐Care Research
Authors: Garris Bill R. , Weber Amy J. 
Published: August 2018

AbstractThis article reviews the presence of family theory in health‐care research. First, we demonstrate some disconnect between models of the patient, which tend to focus on the individual, and a large body of research that finds that relationships influence health. We summarize the contributions of family science and medical family therapy and conclude that family science models and measures are generally underutilized. As a result, practitioners do not have access to the rich tool kit of lenses and interventions offered by systems thinking. We propose several possible ways that family scientists can contribute to health‐care research, such as using the family as the unit of analysis, exploring theories of the family as they relate to health, and suggesting greater involvement of family scientists in health research.
Conceptualizing Family Structure in a Social Determinants of Health Framework
Authors: Russell Luke T. , Coleman Marilyn , Ganong Lawrence 
Published: August 2018

AbstractIn this article, we propose the conceptualization of family structure as an important socially stratified grouping within a social determinants of health (SDOH) framework. We explore how family science literature supports this conceptualization of family structure through evidence of inequity in health outcomes across structurally diverse families that can and ought to be addressed; social stratification across family structures in which some family forms have greater prestige and power than others; and internalized, personally mediated, and institutional prejudices and discriminations that operate on the basis of these social stratifications. Ultimately, we contend that conceptualizing family structure as a socially stratified grouping within an SDOH framework can help enhance family science scholarship and assist family professionals in more effectively serving contemporary families.
Evidence Required to Know Whether Marriage Promotion Increases Other Social Benefits
Authors: Feld Scott L. , Meyer Jordan A. 
Published: August 2018

AbstractFor marriage promotion interventions to be effective, social policy for increasing other social benefits (e.g., child welfare, family financial self‐sufficiency), they must increase numbers and/or quality of marriages in such a way to produce those other social benefits. Evidence should show that particular interventions (a) produce increases in numbers and/or quality of marriages, (b) produce increases in other benefits, and (c) produce increases in other benefits through the changes in marriages described in (a). There is some evidence that marriage promotion interventions have produced small increases in numbers and quality of marriages, but there is little evidence regarding the extent to which these interventions have produced other intended social benefits. We describe the nature of additional data and analyses that would enable us to determine the extent to which marriage promotion interventions are effective ways to increase other social benefits.
Integrating Family Ritual and Sociocultural Theories as a Framework for Understanding Mealtimes of Families With Children on the Autism Spectrum
Authors: Curtiss Sarah L. 
Published: August 2018

AbstractBased on a critical review of research on family meals and autism, this article explores ritual and sociocultural perspectives on shared family meals of families with children on the autism spectrum. Family ritual theory is a lens for analytically understanding phenomena observed in family life, how individuals are connected, and how symbolic meaning is created through patterned behavior. Sociocultural theory explains the presence of culture in thinking, the nature of learning, and the relation between development and instruction. An integration of these theories provides a framework for understanding what mealtime rituals mean and how they are created. Specifically, I address the limitations that impairments associated with autism may impose on a family's ability to have shared family meals by situating sociocultural theory as a method for including children with autism in family rituals when they are not naturally occurring events.
Antibody‐based methods for the measurement of α‐synuclein concentration in human cerebrospinal fluid – method comparison and round robin study
Authors: Mollenhauer Brit , Bowman Frederick DuBois , Drake Daniel , Duong Jimmy , Blennow Kaj , El‐Agnaf Omar , Shaw Leslie M. , Masucci Jennifer , Taylor Peggy , Umek Robert M. , Dunty Jill M. , Smith Chris L. , Stoops Erik , Vanderstichele Hugo , Schmid Adrian W. , Moniatte Marc , Zhang Jing , Kruse Niels , Lashuel Hilal A. , Teunissen Charlotte , Schubert Tanja , Dave Kuldip D. , Hutten Samantha J. , Zetterberg Henrik 
Published: August 2018

Abstractα‐Synuclein is the major component of Lewy bodies and a candidate biomarker for neurodegenerative diseases in which Lewy bodies are common, including Parkinson's disease and dementia with Lewy bodies. A large body of literature suggests that these disorders are characterized by reduced concentrations of α‐synuclein in cerebrospinal fluid (CSF), with overlapping concentrations compared to healthy controls and variability across studies. Several reasons can account for this variability, including technical ones, such as inter‐assay and inter‐laboratory variation (reproducibility). We compared four immunochemical methods for the quantification of α‐synuclein concentration in 50 unique CSF samples. All methods were designed to capture most of the existing α‐synuclein forms in CSF (‘total’ α‐synuclein). Each of the four methods showed high analytical precision, excellent correlation between laboratories (R2 0.83–0.99), and good correlation with each other (R2 0.64–0.93), although the slopes of the regression lines were different between the four immunoassays. The use of common reference CSF samples decreased the differences in α‐synuclein concentration between detection methods and technologies. Pilot data on an immunoprecipitation mass spectrometry (IP‐MS) method is also presented. Our results suggest that the four immunochemical methods and the IP‐MS method measure similar forms of α‐synuclein and that a common reference material would allow harmonization of results between immunoassays.
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Adenomatous polyposis coli‐stimulated GEF 1 (Asef1) is a negative regulator of excitatory synaptic function
Authors: Oh Jun‐Young , Lim Chae‐Seok , Yoo Ki‐Seo , Park Hyungju , Park Young Seok , Kim Eung‐Gook , Lee Yong‐Seok , Kaang Bong‐Kiun , Kim Hyong Kyu 
Published: August 2018

Abstract
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Guanine nucleotide exchange factors (GEFs) play important roles in many cellular processes, including regulation of the structural plasticity of dendritic spines. A GEF protein, adenomatous polyposis coli‐stimulated GEF 1 (Asef1, ARHGEF4) is highly expressed in the nervous system. However, the function of Asef1 has not been investigated in neurons. Here, we present evidence showing that Asef1 negatively regulates the synaptic localization of postsynaptic density protein 95 (PSD‐95) in the excitatory synapse by inhibiting Staufen‐mediated synaptic localization of PSD‐95. Accordingly, Asef1 expression impairs synaptic transmission in hippocampal cultured neurons. In addition, neuronal activity facilitates the dissociation of Asef1 from Staufen in a phosphoinositide 3 kinase (PI3K)‐dependent manner. Taken together, our data reveal Asef1 functions as a negative regulator of synaptic localization of PSD‐95 and synaptic transmission.
Gender Gap in Repartnering: The Role of Parental Status and Custodial Arrangements
Authors: Di Nallo Alessandro 
Published: August 2018

Objective: This study assesses whether parenthood influences repartnering for women and men and explores how repartnering is associated with parental status of the prospective partners.Background: Previous research has not demonstrated whether gender differences in repartnering are conditional on the presence of children. This study aims to better disentangle the specific gender differentials in repartnering probabilities conditional on parenthood and child custody status.Method: The analytical sample consists of 5,372 women and 3,375 men who reported at least one partnership dissolution in the British Understanding Society survey. Multilevel event history models with Markov Chain Monte Carlo simulations are used to estimate the probabilities of (a) finding a new partner and (b) finding a new childless partner or a new partner who has children.Results: The results suggest that mothers, and to a lesser extent fathers, are less likely to repartner than their childless counterparts. Among parents who have child custody, there emerges a distinct gender gap because mothers exhibit a significantly lower rate of repartnering than fathers. Finally, coresident single parents are relatively less likely to repartner with childless individuals, and single fathers more frequently form two‐parent stepfamilies than do mothers.Conclusion: This suggests the presence of a gender divide in repartnering that is especially apparent when child custody is taken into account. The presence of children also reduces the possibility of forming unions with childless individuals.
Interparental Conflict Management Strategies and Parent–Adolescent Relationships: Disentangling Between‐Person From Within‐Person Effects Across Adolescence
Authors: Mastrotheodoros Stefanos , Van der Graaff Jolien , Deković Maja , Meeus Wim H. J. , Branje Susan J. T. 
Published: August 2018

Objective: This study investigates the longitudinal, cross‐lagged associations among interparental conflict management strategies and the parent– adolescent relationship.Background: The following three main hypotheses explain how interparental conflict affects parent–adolescent relationship: the spillover, the compensatory, and the compartmentalization hypotheses. A common key aspect of these hypotheses is the focus on changes within a family; they hypothesize what happens within a family when interparental conflict shakes the family's equilibrium. Although extant research supported the spillover hypothesis, this key aspect was often ignored, and conclusions were based on comparing families with each other. This study investigated how interparental conflict is longitudinally associated with the quality of the parent–child relationship, controlling for stable between‐family differences.Method: Data consisted of six waves of an ongoing study with 497 Dutch adolescents (M = 13.03, 43.1% girls), their mothers, and their fathers. Parents reported on conflict strategies; parents and adolescents reported on parental support, parent–adolescent negative interaction, and parental behavioral control. Random‐intercept cross‐lagged panel models were applied.Results: Most associations were found at the between‐person level: Destructive conflict was related to poor parent–adolescent relationships. Few within‐person associations were found: Changes in destructive conflict only were associated positively with changes in father–adolescent negative interaction.Conclusions: Associations between interparental conflict and the parent–adolescent relationship are mostly due to stable between‐family differences. Intrafamilial fluctuations occur in conflict and the parent–adolescent relationship, but these changes do not predict each other.
Topical emulsion containing pyrrolidine dithiocarbamate: effectiveness against ultraviolet B irradiation-induced injury of hairless mouse skin
Authors: Martinez Renata M , Ivan Ana L M , Vale David L , Campanini Marcela Z , Ferreira Vitor S , Steffen Vinicius S , Vicentini Fabiana T M C , Vilela Fernanda M P , Fonseca Maria J V , Baracat Marcela M , Georgetti Sandra R , Verri Waldiceu A , Casagrande Rúbia 
Published: August 2018

Abstract

Objectives
To evaluate the effects of a topical emulsion containing pyrrolidine dithiocarbamate (PDTC) (EcPDTC) in skin oxidative stress and inflammation triggered by ultraviolet B (UVB) irradiation (dose of 4.14 J/cm2).

Methods
Hairless mouse received treatment with 0.5 g of EcPDTC or control emulsion (CTRLE) on the dorsal surface skin 12 h, 6 h and 5 min before and 6 h after the irradiation. Oxidative stress was evaluated by ferric reducing antioxidant power (FRAP), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS) scavenging capacity, reduced glutathione quantitation, catalase activity, superoxide anion production and lipid peroxidation products. Inflammation parameters were as follows: skin oedema, myeloperoxidase activity (neutrophil marker), matrix metalloproteinase-9 activity, collagen fibre damage, mast cell and sunburn cell counts, and cytokine production.

Key findings
Topical treatment with EcPDTC protected from UVB-induced skin injury by maintaining the antioxidant capacity levels similar to non-irradiated control group. Furthermore, EcPDTC inhibited UVB irradiation-induced superoxide anion production, lipid peroxidation and reduced skin inflammation by inhibiting skin oedema, neutrophil recruitment, metalloproteinase-9 activity, collagen fibre damage, mast cell and sunburn cell counts, and cytokine (TNF-α and IL-1β) production.

Conclusions
Topical treatment with EcPDTC improves antioxidant systems and inhibits inflammation, protecting the skin from the damaging effects of UVB irradiation.
Survey Satisficing Biases the Estimation of Moderation Effects
Authors: Miura Asako , Kobayashi Tetsuro 
Published: August 2018

AbstractSurvey satisficing in online data collection biases the estimation of treatment effects in many ways. Extending the findings of a previous study, which demonstrated that satisficing biased the estimation of main treatment effects, this study also shows that satisficing distorts the estimation of moderation effects. Targeting Japanese adults’ attitudes toward food, this study tests how the effect of country of production (Japan vs. China) is moderated by preexisting ethnocentric attitudes. The results show that while nonsatisficers predictably adjust their attitude toward food based on their preexisting ethnocentric attitude, satisficers stick to their initial stereotypical response. That is, the theoretically predicted moderation effect was observed among nonsatisficers, but not among satisficers, which indicates that satisficing biases not only the estimation of a treatment effect but also that of a moderation effect.
The Enduring Influence of Religion on Senators’ Legislative Behavior
Authors: Arnon Daniel 
Published: August 2018

AbstractDoes a senator's personal religion influence their legislative behavior in the Senate? To date, empirical research has answered this question only using senators’ religious traditions, while more concurrent work implies that religion should be measured as a multifaceted phenomenon. This study tests this proposition by compiling a unique data set of senators’ religion, conceptualized and measured by three different elements—belonging, beliefs, and behavior. The study estimates the association between these three religious facets and senators’ legislative behavior on economic, social, and foreign policy issues, while controlling for their constituencies’ political and religious preferences. It finds that religious beliefs are a strong predictor of senators’ legislative behavior, while religious tradition and behavior are mostly not. Furthermore, it finds that religious beliefs are associated with legislative behavior across a wide array of policy areas and are not confined to sociocultural issues.