bluuebunny/crossref_metadata_2025_split huggingface dataset:
The globe transcriptome analysis reveals hybrid effects and regulatory divergence between (Acanthopagrus schlegelii ♂× Pagrus major ♀) hybrid F1 and parents
Authors: Chen Shuyin , Zhang Zhiyong , Ji Hongjiu , Xu Shixia , Yang Yunxia , Jia Chaofeng , Zhu Fei , Meng Qian , Sun Ruijian , Zhang Zhiwei , Zhao Yongchao 
Published: August 2019

Abstract
Background: Hybridization in two related homoploid species can mix the genotypes which have the potential for good environmental adaptation conditions or rapid growth condition. Since hybridization technique has been used in other animals to produce more rapidly growing individuals, it may be useful in commercial fish culture. The nutritional composition and rapid growth rate of P. major, A. schlegelii and hybrid F1 had been researched. However, gene expressions of these fish are less well studied. de novo assembly RNA-Seq is a better approach to understanding transcriptomes of animals and can offer much data with better coverage and sufficient sequence depth for transcriptomes. Results: In order to facilitate molecular research in parents and hybrids F1, we characterized the whole body transcriptome for identifcation of transcripts involved in growth, metabolism and immune since several attributes were coming from different samples (Acanthopagrus schlegelii, Pagrus major and hybrid F1 Acanthopagrus schlegelii ♂× Pagrus major ♀). We analyzed the transcriptome of As, Pm and hybrid F1 AP using Illumina high throughput sequencing platform. Our results suggested that physiological index of organism development we observed is consistent to the gene express in transcriptome. We have also compared the expression levels of transcripts in parents and hybrids F1. The results showed that AP had a quickly growth and strong resistant than parents Pm and As. Conclusion: This is the first research on transcriptome of As, Pm and hybrid F1 at one month age. And it will provide an important basic molecular data for studying molecular mechanisms involved in different sample.
Validation of an equine serum amyloid A assay with an unusually broad working range
Authors: Jacobsen Stine , Vinther Anne Mette , Kjelgaard-Hansen Mads , Nielsen Lise Nikolic 
Published: August 2019

Abstract
Background

Serum amyloid A (SAA) is a major equine acute phase protein and of great value in detection and monitoring of inflammation. A new immunoturbidometric assay based on monoclonal antibodies (VET-SAA, Eiken Chemical Co., Japan) may be useful for SAA measurements in routine diagnostic laboratories. The aim of the study was to validate the VET-SAA immunoturbidometric assay and use it to measure serum SAA concentrations in a variety of clinical cases. Precision was assessed by intra- and interassay coefficients of variation of repeated measurements of serum pools (low, intermediate, high concentrations of SAA). Accuracy was estimated by linearity under dilution. Detection limit was determined by replicate determinations of ionized water. Measurements were compared to measurements performed in a previously validated SAA assay (LZSAA assay, Eiken Chemical Co., Japan). Subsequently, the VET-SAA assay was used for measuring serum SAA concentrations in horses with and without inflammation.

Results

Detection limit was 1.2 mg/L. Without modifications, the assay measured SAA concentrations with acceptable reliability in a broad concentration range (0 to > 6000 mg/L). In the 0-3000 mg/L range, the assay demonstrated good precision and accuracy, and concentrations correlated well with those obtained in the LZSAA assay, albeit with a slight systematic bias. Concentrations of SAA assessed in horses with and without inflammation followed the expected pattern, with significantly higher concentrations in horses with systemic inflammation than in healthy horses and horses with non-inflammatory disease.

Conclusions

The assay was unique in its ability to measure SAA concentrations with acceptable reliability over an extreme concentration range. This is relevant in the equine species, where SAA concentrations may reach very high concentrations.
Clinical characteristics and post-discharge follow-up analysis of 10 cases of congenital tuberculosis: a retrospective observational study
Authors: Du Juan , Dong Shixiao , Jia Shengnan , Zhang Qiaoru , Hei Mingyan 
Published: August 2019

Abstract
Background
Congenital tuberculosis (TB) is a rare disease with a high mortality. In this study, we reviewed patients with congenital TB that were promptly treated with an anti-TB regimen, including linezolid, based on new clinical diagnosis criteria. We described the clinical manifestations, treatment, and long-term prognosis of this disease in these patients.
Methods
This was a retrospective observational study that enrolled patients with congenital TB that were admitted to Beijing Children’s Hospital between 2009 and 2018.
Results
A total of 10 congenital TB patients were enrolled. Ninety-percentage of the mothers were diagnosed at postpartum; however, none of the mothers received anti-TB treatment during pregnancy. The onset age of congenital TB ranged from 1 to 53 days. Chest computed tomography scans demonstrated pulmonary nodules in 100% of the patients and mediastinal adenopathy in 40% of the patients. Abdominal ultrasonography showed hypoechoic nodules in the liver and spleen in 40% of the patients. The positive rate of GeneXpert and T-spot were relatively high—80% and 78%, respectively. Anti-TB treatment was started when congenital TB was confirmed for 1 patient based on TB-specific tests or clinically diagnosed for the other 9 patients based on confirmed maternal TB history and typical radiological findings. Beside isoniazid, rifampicin, and pyrazinamide, linezolid was also administrated in 70% of the patients for a period of 2–6 months. One patient was reported to present with spontaneously resolved thrombocytopenia. The survival rate was 100%, and 33% of the patients have thrived and reached normal developmental milestones since the last documented follow-up.
Conclusion
Congenital TB is a potentially fatal disease, but early diagnosis based on maternal history, typical imaging results, and timely treatment can improve the outcome. Linezolid is safe and might be effective in reducing the mortality of congenital TB.
Post-Discharge survival status and predictors of mortality among severely acute malnourished children &lt;5 years of age admitted to Minia University Maternity and Children Hospital
Authors: Ghazawy Eman , Bebars Gihan , Eshak Ehab Salah 
Published: August 2019

Abstract
Background: Though effective treatment programs for severely malnourished children are available, but little is known about long-term outcomes and potential predictors of post-discharge mortality. The aim of this study was to assess the post-discharge survival status and predictors for post-discharge mortality in severely malnourished children admitted to Minia University Maternity and Children Hospital Methods: A retrospective cohort study which included 135 children under five years of age who were admitted to the nutrition rehabilitation ward with severe acute malnutrition (SAM) during the period from January to December 2018. Data were collected from the inpatients hospital records and children’s parents/guardians were interviewed using a detailed structured questionnaire that inquired about demographic and socioeconomic variables. Results: A total of 135 children were enrolled into the study. Death rate during hospitalization was 9.6%. The cumulative probability of survival beyond eight weeks and at least for 24 weeks after discharge was 89.3% with a cumulative probability of post-discharge mortality of 10.7% and all deaths occurred within eight weeks after discharge. The main predictors for post-discharge mortality were illiteracy of mothers and the presence of edema at admission; the multivariable HRs (95%CIs) were 7.10 (1.58-31.93; p=0.01) and 6.96 (0.84-357.85; p=0.07), respectively. Conclusions: Mothers’ education and edema at admission are independent predictors for post-discharge mortality in under-five children with SAM. The identification of predictors for post-discharge mortality is an important preliminary step for interventions aiming to reduce morbidity and mortality following discharge.
Cerebrovascular events in hemodialysis patients; 3-year observational study
Authors: Ozelsancak Ruya , Micozkadioglu Hasan , Torun Dilek , Tekkarismaz Nihan 
Published: August 2019

Abstract
Purpose

This study reports findings in subjects who underwent brain imaging for any reason, and examined factors influencing cerebrovascular events (CVEs) in hemodialysis (HD) patients.

Methods

A total of 432 patients who underwent HD for at least 5 months were included in the study; 264 had been examined by cerebral computed tomography or magnetic resonance imaging examination within the previous 3 years. Cerebrovascular pathology was detected in 139 of 264 patients.

Results

Of the 139 patients, 65 (24.62%) had ischemic lesions, 25 (9.47%) had hemorrhagic lesions, and 49 (18.56%) had cerebral small vessel disease (CSVD). We compared recorded data and later clinical findings between patients with and those without CVEs. The cause of end-stage renal disease was diabetes in 58.5% of patients with ischemic lesions, 52% in those with hemorrhagic lesions, and 55% in those with CSVD (P < 0.05). Patients with cerebrovascular ischemia were older (P = 0.0001) and had lower serum creatinine (sCr) (P = 0.0001) and higher serum C-reactive protein (CRP) (P = 0.002) levels than normal subjects. Hemorrhagic patients were older (P = 0.003) and had lower sCr (P = 0.003) and serum predialysis potassium (P = 0.003) and parathyroid hormone (PTH) (P = 0.004) levels than normal subjects. Patients with CSVD were older (P < 0.0001) and had lower sCr (P < 0.0001), phosphorus (P < 0.007), and PTH (P < 0.013) and higher CRP (P < 0.002) levels than normal subjects.

Conclusion

HD patients with CVEs are older and typically have diabetes mellitus and lower sCr levels.
Multiple chronic conditions and associated health care expenses in US adults with cancer: A 2010-2015 Medical Expenditure Panel Survey study
Authors: Davis-Ajami Mary Lynn , Lu Zhiqiang K , Wu Jun 
Published: August 2019

Abstract
Abstract
Background: We know cancer increases the risk of developing one or more chronic conditions, yet little research describes the associations between health care costs, utilization patterns, and chronic conditions in adults with cancer. The objective of this study was to examine the treated prevalence of chronic conditions and the effect of chronic conditions on health care expenses in US adults with cancer.
Methods: This retrospective observational study used US Medical Expenditure Panel Survey (MEPS) Household Component (2010-2015) data sampling adults diagnosed with cancer and one or more of 18 select chronic conditions. The measures used were treated prevalence of chronic conditions, and total and chronic condition-specific health expenses (per-person, per-year). Generalized linear models assessed chronic condition-specific expenses in adults with cancer vs. without cancer and the effect of chronic conditions on total health expenses in adults with cancer, respectively, by controlling for demographic and health characteristics. Accounting for the complex survey design in MEPS, all data analyses and statistical procedures applied longitudinal weights for national estimates.
Results: Among 3,657 eligible adults with cancer, 83.9% (n = 3,040; representing 16 million US individuals per-year) had at least one chronic condition, and 29.7% reported four or more conditions. Among those with cancer, hypertension (59.7%), hyperlipidemia (53.6%), arthritis (25.6%), diabetes (22.2%), and coronary artery disease (18.2%) were the five most prevalent chronic conditions. Chronic conditions accounted for 30% of total health expenses. Total health expenses were $6,388 higher for those with chronic conditions vs. those without (p<0.001). Health expenses associated with chronic conditions increased by 34% in adults with cancer vs. those without cancer after adjustment.
Conclusions: In US adults with cancer, the treated prevalence of common chronic conditions was high and health expenses associated with chronic conditions were higher than those without cancer. A holistic treatment plan is needed to improve cost outcomes.
Keywords: cancer, multiple chronic conditions, health expenses, health utilization
On the path towards universal coverage of hepatitis C treatment among people receiving opioid agonist therapy (OAT) in Norway: a prospective cohort study from 2013 to 2017
Authors: Aas Christer , Vold Jørn Henrik , Skurtveit Svetlana , Odsbu Ingvild , Chalabianloo Fatemeh , Økland Jan-Magnus , Leiva Alexander , Vickerman Peter , Johansson Kjell Arne , Fadnes Lars Thore 
Published: August 2019

Abstract
Background

The development of new direct-acting antiviral (DAA) medications together with opioid agonist therapy (OAT) have been put forward as having an important role in the management and elimination of chronic hepatitis C (HCV) infection. Yet, HCV treatment uptake and coverage have remained low among OAT populations. We aimed to calculate cumulative HCV treatment coverage among individuals enrolled in OAT in Norway between 2013 and 2017, and to document the treatment transition to interferon-free regimens. Moreover, we aimed to describe adherence to DAAs in the same cohort.

Methods

This observational study was based on data from The Norwegian Prescription Database (NorPD). We studied dispensed OAT and HCV treatment annually to calculate the cumulative frequency, and employed secondary sources to further calculate prevalence, incidence and HCV treatment coverage from 2013 to 2017, among the OAT population. Factors associated with adherence to DAAs were identified a priori and subject to logistic regression.

Results

10,371 individuals were identified with dispensed OAT, 1,475 individuals of these with dispensed HCV treatment. Annual HCV treatment coverage increased from 3.5% (95% CI: 3.2-4.4) in 2013 to 17% (95% CI: 17-20) in 2017, giving a cumulative HCV coverage among OAT patients in Norway of 38.5%. A complete shift to interferon-free treatment regimens occured, where DAAs accounting for 32% of HCV treatments in 2013 and 99% in 2017. About two-thirds of OAT patients were considered adherent to their DAA regimens across all genotypes. High-level of OAT continuity was associated with improved adherence to DAAs (aOR 1.4, 95% CI: 1-2, p=0.035).

Conclusions

A large increase in HCV treatment coverage attributed by a complete shift to interferon-free regimens among the Norwegian OAT population has been demonstrated. However, a further substantial scale-up in HCV treatment is required to reach the universal targets of controlling and eradicating the HCV endemic.
Epidemiology and comorbidities of patients with rheumatoid arthritis in Spain during the period 2002-2017
Authors: Del-Moral-Luque Juan Antonio , Gil-Conesa Mario , Gil-Prieto Ruth , Gil-de-Miguel Ángel , Mazzuccheli-Esteban Ramón , Caravaca Gil Rodriguez 
Published: August 2019

Abstract
Background: Rheumatoid arthritis (RA) is a chronic autoimmune rheumatic disease that is associated with multiple comorbidities and has a significant economic impact on the Spanish health system. The objective of this study was to estimate the hospital burden of rheumatoid arthritis, its variants and their associated comorbidities.

Methods: Observational descriptive study that reviewed hospital records from the CMBD. We included all hospitalizations of patients in Spain whose main diagnosis or comorbidity in the ICD-9-CM was rheumatoid arthritis during the period of 2002-2017.

Results: A total of 315,190 hospitalizations with the RA code were recorded; 67.3% were in women. The mean age of the patients was 68.5 ± 13.9 years. The median length of hospital stay was 7 days (IQR 3-11 days). In 29,809 of the admissions, RA was coded as the main diagnosis (9.4%). When RA was not coded as the main diagnosis, the most frequent main diagnoses were diseases of the circulatory system (18.9%) and diseases of the respiratory system (17.4%). The hospitalization rate during the period of 2002-2017 was 43.8 (95% CI: 43.7-44.0) per 100,000 inhabitants. The total cost for the healthcare system was 1.476 million euros, with a median of 3,542 euros per hospitalization (IQR 2,646-5,222 euros).

Conclusions: In Spain, the highest hospitalization rates for RA were registered within the continental climate region. The hospitalization rate of patients with RA increased during the study period, despite the decrease in the hospitalization rate when RA was the main diagnosis.
Socio-Economic Benefit of Lake Ecosystem (In Case of Lake Ziway)
Authors: Gebremedhin Gezahegne Seyoum , Belliethathan Satishkumer 
Published: August 2019

This study focused on the socio-economic benefit of the Western shoreline of Lake Ziway ecosystem. Lake Ziway great importance for food and water for both groups of respondents and additional sources of raw material, energy, cultivation, organic fertilizers, genetic and medicinal plants. Lake Ziway also has a major economic benefit for both groups of respondents. The respondent fishermen caught a mean amount of 2,524Kg per year with a minimum and maximum amount of fish 504Kg and 16,800Kg per year respectively and with this fish-catching, they got an average income of 51,398 Birr ($2,570) per year with range of 7,200 Birr ($360) and 288,000 Birr ($14,400) per year. As like of fishermen small scale irrigation also got economic benefit with their production of cereal crops, fruits, and vegetables. They produce in average 13.47Quintal of cereal crop and 69.56Quintal of fruits and vegetables per year and they got an average income of 7,727 Birr ($386) and with a range of 13,714 Birr ($686) per year respectively. this incurred that wetland ecosystem has a lot of socio-economic benefit for the people live nearby especially for developing countries like Ethiopia they are more dependent on a natural ecosystem like of Lake Ziway.
René Girarda teoria mimetyczna. Między religią a polityką
Authors: Romejko Adam 
Published: August 2019

W świecie zachodnim popularne jest przekonanie, że sfera polityczna i religijna (państwowa i kościelna) są niezależne (por. Mt 22,21). Uczonym, który uważał, że u początków ludzkiej kultury (cywilizacji) było inaczej, jest francuski antropolog i literaturoznawca René Girard. Podkreśla on, że religia (rytuał ofiarniczy) stoi u podstaw ludzkich instytucji, w tym tych o politycznym charakterze. W wypracowanej teorii, określanej mianem mimetycznej, zwraca uwagę na przemoc, która w kolektywnej formie jest w stanie budować i podtrzymywać egzystencję ludzkiej wspólnoty. W teorii mimetycznej pojawiają się cztery kwestie korespondujące z polityką: 1) budowanie politycznej wspólnoty na podstawie niechęci do innych (jednostki, grupy mniejszościowej); 2) wykorzystanie ludzkiego mimetyzmu w kierowaniu grupami społecznymi; 3) pozycjonowanie siebie jako ofiary, które ma na celu zyski polityczne itp.; 4) nowoczesna troska o ofiary, stanowiąca oręż wobec politycznych przeciwników. Teorię mimetyczną można stosować w badaniach politycznych fenomenów. W niniejszym artykule zanalizowano za pomocą „Girardowskich okularów” niemiecki talk-show maybrit illner z dn. 2 listopada 2017 r
Expression of Thanatin in HEK293 Cells and Investigation of its Antibacterial Effects on Some Human Pathogens
Authors: Tanhaeian Abbas , Azghandi Marjan , Mousavi Zahra , Javadmanesh Ali 
Published: August 2019

Background:Thanatin is the smallest member of Beta-hairpin class of cationic peptide derived from insects with vast activities against various pathogens.Objective:n this study, the antimicrobial activity of this peptide against some species of human bacterial pathogens as well as its toxicity on NIH cells were evaluated.Method:Thanatin DNA sequence was cloned into pcDNA3.1+ vector and transformed into a DH5α bacterial strain. Then the recombinant plasmids were transfected into HEK-293 cells by calcium phosphate co-precipitation. After applying antibiotic treatment, the supernatant medium containing thanatin was collected. The peptide quantity was estimated by SDS-PAGE and GelQuant software. The antimicrobial activity of this peptide was performed with Minimum Inhibitory Concentration (MIC) method. In addition, its toxicity on NIH cells were evaluated by MTT assay.Results:The peptide quantity was estimated approximately 164.21 µmolL-1. The antibacterial activity of thanatin was estimated between 0.99 and 31.58 µmolL-1 using MIC method. The result of cytotoxicity test on NIH cell line showed that the peptide toxicity up to the concentration of 394.10 µmolL-1 and for 48 hours, was not statistically significant from negative control cells (P>0.05). The antimicrobial assay demonstrated that thanatin had an antibacterial effect on some tested microorganisms. The results obtained in this study also showed that thanatin had no toxicity on mammalian cell lines including HEK293 and NIH.Conclusion:Antimicrobial peptides such as thanatin are considered to be appropriate alternatives to conventional antibiotics in treating various human pathological diseases bacteria.
Genes Expression Classification Through Histone Modification Using Temporal Neural Network
Authors: Nair Rajit , Bhagat Amit 
Published: August 2019

Background:
Genes expression is high dimensional data, so it is very difficult to classify
high dimensional data through traditional machine learning approaches. In this work we have proposed
a model based on combined approach of Convolutional Neural Network and Recurrent Neural
Network, both belong to deep learning model. The prediction has shown improved result than other
machine learning algorithms. Expressions are generated through histone modification.

Methods:
To improve the accuracy deep learning model is proposed i.e. based on Convolutional and
Recurrent neural network. This proposed model uses filter, causal convolutional layers and Residual
Block for predictions.

Results:
In this work we have implemented the machine learning algorithms and deep learning algorithms
like Logistic Regression, SVM, CNN, Deep Chrome and the proposed Temporal Neural
Network. The performance is measured on the basis of parameters like accuracy, precision and AUC
on the training and testing set.

Conclusion:
The proposed Temporal Neural Network model has shown better performance than
other machine learning and deep learning algorithms. Due to this proposed deep learning algorithm
can be successfully applied on the genes expression dataset.