bluuebunny/crossref_metadata_2025_split huggingface dataset:
An easy-to-assemble, robust, and lightweight drive implant for chronic tetrode recordings in freely moving animals
Authors: Voigts Jakob , Newman Jonathan P. , Wilson Matthew A. , Harnett Mark T. 
Published: August 2019

AbstractTetrode arrays are the gold-standard method for neuronal recordings in many studies with behaving animals, especially for deep structures and chronic recordings. Here we outline an improved drive design for use in freely behaving animals. Our design makes use of recently developed technologies to reduce the complexity and build time of the drive while maintaining a low weight. The design also presents an improvement over many existing designs in terms of robustness and ease of use. We describe two variants: a 16 tetrode implant weighing ∼2 g for mice, bats, tree shrews and similar animals, and a 64 tetrode implant weighing ∼16 g for rats, and similar animals.These designs were co-developed and optimized alongside a new class of drive-mounted feature-rich amplifier boards with ultra-thin RF tethers, as described in an upcoming paper (Newman, Zhang et al., in prep). This design significantly improves the data yield of chronic electrophysiology experiments.
Proteinase-Activated Receptor 4 (PAR4) Activation Triggers Cell Membrane Blebbing through RhoA and β-arrestin
Authors: Vanderboor Christina MG , Thibeault Pierre E , Nixon Kevin CJ , Gros Robert , Kramer Jamie , Ramachandran Rithwik 
Published: August 2019

AbstractProteinase-Activated Receptors (PARs) are a four-member family of G-protein coupled receptors that are activated via proteolysis. PAR4 is a member of this family that is cleaved and activated by serine proteinases such as thrombin, trypsin and cathepsin-G. PAR4 is expressed in a variety of tissues and cell types including platelets, vascular smooth muscle cells and neuronal cells. In studying PAR4 signalling and trafficking, we observed dynamic changes in the cell membrane with spherical membrane protrusions that resemble plasma membrane blebbing. Since non-apoptotic membrane blebbing is now recognized as an important regulator of cell migration, cancer cell invasion, and vesicular content release we sought to elucidate the signalling pathway downstream of PAR4 activation that leads to such events. Using a combination of pharmacological inhibition and CRISPR/Cas9-mediated gene-editing approaches we establish that PAR4-dependent membrane blebbing occurs independently of the Gαq/11and Gαisignalling pathways and is dependent on signalling via the β-arrestin-1/-2 and RhoA signalling pathways. In order to gain a more comprehensive understanding of β-arrestin-mediated signalling downstream of PAR4 and to guide future studies, we undertook RNA-seq analysis of PAR4 activated genes in control cells and in cells lacking β-arrestin-1/-2. A list of differentially expressed genes was generated followed by Gene Ontology (GO) and enrichment analysis, revealing PAR4 regulation of genes involved in processes including blood coagulation and circulation, cell-cell adhesion, sensory perception and neuron-neuron synaptic transmission-terms that relate back to known functions of PAR4 and are consistent with our finding of membrane blebbing triggered by PAR4 activation. Together these studies provide further mechanistic insight into PAR4 regulation of cellular function.Significance StatementWe find that the thrombin receptor PAR4 triggers cell membrane blebbing in a RhoA- and β-arrestin-dependent manner. In addition to identifying novel cellular responses mediated by PAR4, these data provide further evidence for biased signaling in PAR4 since membrane blebbing was dependent on some, but not all, signaling pathways activated by PAR4. Finally through CRISPR/Cas9-mediated targeting and RNA-seq analysis we catalogue here PAR4-dependent transcription that is dependent on β-arrestin.
Engineering “self-homing” circulating tumour cells as novel cancer theranostics
Authors: Parkins Katie M , Dubois Veronica P , Kelly John J , Chen Yuanxin , Foster Paula J , Ronald John A 
Published: August 2019

AbstractPurpose: New ways to target and treat metastatic disease are urgently needed. Tumor “self-homing” describes the recruitment of circulating tumor cells (CTCs) back to a previously excised primary tumor location, contributing to tumor recurrence, as well as their migration to established metastatic lesions. Recently, self-homing CTCs have been exploited as delivery vehicles for anti-cancer therapeutics in preclinical primary tumor models. However, the ability of CTCs to self-home and treat metastatic disease is largely unknown. Methods: Here, we employ molecular imaging to explore whether systemically-administered CTCs home to metastatic lesions and if CTCs armed with both a reporter gene and a cytotoxic prodrug gene therapy can be used to visualize and treat metastatic disease. Results: Bioluminescence imaging (BLI) performed over time revealed a remarkable ability of CTCs to home to primary and metastatic tumors throughout the body. Mice that received therapeutic CTCs had less BLI signal as well as less primary tumour burden than control mice. Preliminary data also showed self-homing therapeutic CTCs may be effective at treating disseminated breast cancer metastases. Conclusion: Using dual-luciferase BLI, this study demonstrates the noteworthy ability of experimental CTCs to home to disseminated breast cancer lesions. Moreover, by incorporating a prodrug gene therapy system into our self-homing CTCs, we show exciting progress towards effective and targeted delivery of gene-based therapeutics to treat both primary and metastatic lesions.
Fatty acid-induced lipotoxicity inhibits choline metabolism independent of ER stress in mouse primary hepatocytes
Authors: O’Dwyer Conor , Yaworski Rebecca , LeBlond Nicholas D. , Ghorbani Peyman , Nunes Julia R.C. , Margison Kaitlyn D. , Smith Tyler T.K. , Odai Kaelan Gobeil , Han Shauna , Fullerton Morgan D. 
Published: August 2019

ABSTRACTCholine is an essential nutrient that is critical component of the membrane phospholipid phosphatidylcholine (PC), the neurotransmitter acetylcholine and the methylation pathway. In the liver specifically, PC is the major membrane constituent and can be synthesized by the CDP-choline or the phosphatidylethanolamine (PE) N-methyltransferase (PEMT) pathway. With the continuing global rise in the rates of obesity and non-alcoholic fatty liver disease, we sought to explore how excess fatty acids (FA), typical of an obesity and hepatic steatosis, affect choline uptake and metabolism in primary hepatocytes. Our results demonstrate that hepatocytes chronically treated with palmitate, but not oleate or a mixture, had decreased choline uptake, which was associated with lower choline incorporation into PC and lower expression of choline transport proteins. Interestingly, a reduction in the rate of degradation spared PC levels in response to palmitate when compared to control. PE synthesis was slightly diminished; however, no compensatory changes in the PEMT pathway were observed. We next hypothesized that ER stress may be a potential mechanism by which palmitate treatment diminished choline. However, when we exposed primary hepatocytes to the common ER stress inducing compound tunicamycin, choline uptake, contrary to our expectation was augmented, concomitant with the transcript expression of choline transporters. Moreover, tunicamycin-induced ER stress divorced the observed increase in choline uptake from CDP-choline pathway flux since ER stress significantly diminished the incorporation and total PC content, similar to PE.Conclusion: Therefore, our results suggest that the altered FA milieu seen in obesity and fatty liver disease progression may adversely affect choline metabolism, but that compensatory mechanisms work to maintain phospholipid homeostasis.Abstract Figure
Characterization of LXR-activating nanoparticle formulations in primary mouse macrophages
Authors: Smith Tyler KT , Kahiel Zaina , LeBlond Nicholas D , Ghorbani Peyman , Farah Eliya , Cote Marceline , Gadde Suresh , Fullerton Morgan D 
Published: August 2019

AbstractActivation of the transcription factor liver X receptor (LXR), has shown to be efficient at curbing aberrant lipid metabolism and inflammation. While small molecule delivery via nanomedicine has promising applications for a number of chronic diseases, there remain questions as to how nanoparticle formulation might be tailored to suit different tissue microenvironments and aid in drug delivery. In the current study, we compared the drug delivery capability of three nanoparticle (NP) formulations encapsulating the LXR activator, GW-3956. We observed little difference in the base characteristics of standard PLGA-PEG NP when compared to two redox-active polymeric NP formulations (DD and DB). Moreover, we also observed similar uptake of these NP into primary mouse macrophages. After an initial acute uptake period and using the transcript and protein expression of the cholesterol efflux protein ATP binding cassette A1 (ABCA1) as a readout, we determined that while the induction of transcript expression was similar between NPs, treatment with the redox-sensitive DB formulation resulted in a higher level of ABCA1 protein 24 h after the removal of the drug-containing NPs. Our results suggest that NP formulations responsive to cellular cues may be an effective tool for targeted and disease-specific drug release.
Interpretation of Phase Boundary Fluctuation Spectra in Biological Membranes with Nanoscale Organization
Authors: Iyer S. S. , Negi A. , Srivastava A. 
Published: August 2019

AbstractIn this work, we use Support Vector Machine algorithm to detect simple and complex interfaces in atomistic and coarse-grained molecular simulation trajectories of phase separating lipid bilayer systems. We show that the power spectral density of the interfacial height fluctuations and in turn the line tension of the lipid bilayer systems depend on the order parameter used to identify the intrinsic interface. To highlight the effect of artificial smoothing of the interface on the fluctuation spectra and the ensuing line tension calculations, we perform a convolution of the boundaries identified at molecular resolution with a 2D Gaussian function of variance ε2 equal to the resolution limit, (1/2πε2)exp(−|r|2/2ε2). The convolution function is given by h⊗g, where h is the instantaneous height fluctuation and g is the Gaussian function. This is similar to the effect of point spread functions in experiments. We find that the region of fluctuation spectra that scales according to capillary wave theory formalism depends on the complexity of the interfacial geometry, which may not always be detected at experimental resolutions. We propose that the different q-regimes in the fluctuation spectra can be used to characterize mode dependent inter-facial tensions to understand the interfaces beyond the linear line tension calculations. This could also be useful in interpretation of fluctuating boundaries in out-of-equilibrium in-vivo membrane systems that carry information about the nature of non-thermal (active) fluctuations in these systems.
Optimization of high pressure processing to reduce the safety risk of low-salt ready-to-eat sliced turkey breast supplemented with carvacrol
Authors: Oliveira Thales Leandro Coutinho de , Haddad Gabriela de Barros Silva , Ramos Alcinéia de Lemos Souza , Ramos Eduardo Mendes , Piccoli Roberta Hilsdorf , Cristianini Marcelo 
Published: August 2019

Purpose
The purpose of this paper is to evaluate the optimization of high hydrostatic pressure (HHP) processing for the microbial inactivation on low-sodium sliced vacuum-packaged turkey breast supplemented with a natural antimicrobial compound (carvacrol).

Design/methodology/approach
A response surface methodology was used to model and describe the effects of different pressures (200–650 MPa) and holding times (30–300 s) during HHP processing of low-salt ready-to-eat turkey breast supplemented with 200 mg/kg of carvacrol on survival of the target pathogen (Listeria sp.) and spoilage microflora and on the quality attributes, including pH, syneresis, CIE color and lipid oxidation.

Findings
The HHP parameters influenced (p<0.05) the lethality rates and syneresis but did not affect the pH values and lipid oxidation of the products evaluated. According to the required performance criteria for Listeria post-lethality treatment, a treatment at 600 MPa/180 s (at 25°C) appears to be suitable for the studied low-sodium product. The HHP bacterial inactivation effects can notably be potentiated via the presence of carvacrol, and is useful at sensory acceptable sub-inhibitory levels.

Originality/value
This study shows that combined HHP plus additives may produce similar safety and shelf-life extension effects with mild HHP treatments, creating a global increase in the quality of HHP-processed food in addition to reducing costs on equipment maintenance and increasing industry productivity.
When do job insecure employees adapt to change?
Authors: Lavigne Kristi N. , Whitaker Victoria L. , Jundt Dustin K. , Shoss Mindy K. 
Published: August 2019

PurposeThe purpose of this paper is to examine the relationship between job insecurity and adaptive performance (AP), contingent on changes to core work tasks, which we position as a situational cue to employees regarding important work behaviors.Design/methodology/approachEmployees and their supervisors were invited to participate in the study. Supervisors were asked to provide ratings of employees’ AP and changes to core tasks; employees reported on job insecurity.FindingsAs predicted, changes to core tasks moderated the relationship between job insecurity and AP. Job insecurity was negatively related to AP for those experiencing low levels of change, but was not related to AP for those experiencing high levels of change. Counter to expectations, no main effect of job insecurity was found.Research limitations/implicationsThis study employed a fairly small sample of workers from two organizations, which could limit generalizability.Practical implicationsThe study identifies changes to core tasks as a boundary condition for the job insecurity–AP relationship. Findings suggest that organizations may not observe deleterious consequences of job insecurity on AP when changes to core tasks are high.Originality/valueFew researchers have examined boundary conditions of the impact of job insecurity on AP. Furthermore, inconsistent findings regarding the link between job insecurity and AP have emerged. This study fills the gap and expands upon previous research by examining changes to core tasks as a condition under which job insecurity does not pose an issue for AP.
Translating sustainability into competitive advantage: the case of Mexico’s hospitality industry
Authors: Gutiérrez-Martínez Isis , Duhamel Francois 
Published: August 2019

PurposeThe purpose of this paper is to explore how business organizations design and implement sustainability practices to foster competitive advantage.Design/methodology/approachThis qualitative study is based on an exploratory multiple case study involving 11 Mexican hotels recognized as “champions” in sustainability. Data gathering took place via in-depth interviews with hotel managers.FindingsThe key internal resources and capabilities must be supported by integrated microfoundations. The interaction between attributes of key resources and microfoundations reveals four transversal dimensions, namely, leadership orientation, organizational culture, team-based organizational structures and human resources and control management systems to build a sustainability-based competitive advantage in the hospitality industry.Practical implicationsThe implementation of sustainability processes in the hospitality industry depends on the ability of leaders to craft systems, build commitment and align interests and goals consistent with sustainability. Business managers may find some guidance for implementing sustainability-related initiatives from the results of this paper.Originality/valueThis work presents both theoretical and practical contributions, integrating and operationalizing a set of key resources (using the VRIO framework) and microfoundations approach to create and maintain a sustainability-based competitive advantage, in contrast to existing studies where those resources and conditions are examined in a piecemeal fashion.
Development of femoral component design geometry by using DMROVAS (design method requiring optimum volume and safety)
Authors: Öztürk Burak , Erzincanli Fehmi 
Published: August 2019

Purpose
This study aims to design a femoral component with minimum volume and maximum safety coefficient. Total knee prosthesis is a well-established therapy in arthroplasty applications. And in particular, with respect to damaged or weakened cartilage, new prostheses are being manufactured from bio-materials which are compatible with the human body to replace these damages. A new universal method (design method requiring optimum volume and safety [DMROVAS]) was propounded to find the optimum design parameters of tibial component.

Design/methodology/approach
The design montage was analyzed via the finite element method (FEM). To ensure the stability of the prosthesis, the maximum stress angle and magnitude of the force on the knee were taken into consideration. In the analysis process, results revealed two different maximum stress areas which were supported by case reports in the literature. Variations of maximum stress, safety factor and weight were revealed by FEM analysis, and ANOVA was used to determine the F force percentage for each of the design parameters.

Findings
Optimal design parameter levels were chosen for the individual’s minimum weight. Stress maps were constructed to optimize design choices that enabled further enhancement of the design models. The safety factor variation (SFV) of 5.73 was obtained for the volume of 39,219 mL for a region which had maximum stress. At the same time, for a maximum SFV and at the same time an average weight, values of 37,308 mL and 5.8 for volume and SFV were attained, respectively, using statistical methods.

Originality/value
This proposed optimal design development method is new and one that can be used for many biomechanical products and universal industrial designs.
On Minkowski difference-based contact detection in discrete/discontinuous modelling of convex polygons/polyhedra
Authors: Feng Y.T. , Tan Yuanqiang 
Published: August 2019

Purpose
Contact detection for convex polygons/polyhedra has been a critical issue in discrete/discontinuous modelling, such as the discrete element method (DEM) and the discontinuous deformation analysis (DDA). The recently developed 3D contact theory for polyhedra in DDA depends on the so-called entrance block of two polyhedra and reduces the contact to evaluate the distance between the reference point to the corresponding entrance block, but effective implementation is still lacking.

Design/methodology/approach
In this paper, the equivalence of the entrance block and the Minkowski difference of two polyhedra is emphasised and two well-known Minkowski difference-based contact detection and overlap computation algorithms, GJK and expanding polytope algorithm (EPA), are chosen as the possible numerical approaches to the 3D contact theory for DDA, and also as alternatives for computing polyhedral contact features in DEM. The key algorithmic issues are outlined and their important features are highlighted.

Findings
Numerical examples indicate that the average number of updates required in GJK for polyhedral contact is around 6, and only 1 or 2 iterations are needed in EPA to find the overlap and all the relevant contact features when the overlap between polyhedra is small.

Originality/value
The equivalence of the entrance block in DDA and the Minkowski difference of two polyhedra is emphasised; GJK- and EPA-based contact algorithms are applied to convex polyhedra in DEM; energy conservation is guaranteed for the contact theory used; and numerical results demonstrate the effectiveness of the proposed methodologies.
Improved moth flame optimization algorithm to optimize cost-oriented two-sided assembly line balancing
Authors: Ab. Rashid Mohd Fadzil Faisae , Mohd Rose Ahmad Nasser , Nik Mohamed Nik Mohd Zuki , Mohd Romlay Fadhlur Rahman 
Published: August 2019

Purpose This paper aims to propose an improved Moth Flame Optimization (I-MFO) algorithm to optimize the cost-oriented two-sided assembly line balancing (2S-ALB). Prior to the decision to assemble a new product, the manufacturer will carefully study and optimize the related cost to set up and run the assembly line. For the first time in ALB, the power cost is modeled together with the equipment, set up and labor costs.

Design/methodology/approach I-MFO was proposed by introducing a global reference flame mechanism to guide the global search direction. A set of benchmark problems was used to test the I-MFO performance. Apart from the benchmark problems, a case study from a body shop assembly was also presented.

Findings The computational experiment indicated that the I-MFO obtained promising results compared to comparison algorithms, which included the particle swarm optimization, Cuckoo Search and ant colony optimization. Meanwhile, the results from the case study showed that the proposed cost-oriented 2S-ALB model was able to assist the manufacturer in making better decisions for different planning periods.

Originality/value The main contribution of this work is the global reference flame mechanism for MFO algorithm. Furthermore, this research introduced a new cost-oriented model that considered power consumption in the assembly line design.
Talent management dimensions and its relationship with Generation Y employee’s intention to quit: an Indian hotel perspective
Authors: Gupta Vikas 
Published: August 2019

Purpose
The purpose of this paper is to determine the causal relationship between talent management dimensions such as perceived organisational support (POS), human capital index and its influence on the turnover intentions of Generation Y employees in Indian hotel industry. This research will also examine how the intension of hotel employees to quit relates to the talent management practices (TMP) in the Indian hotel establishments.

Design/methodology/approach
This research will suggest an integrated conceptual model based on earlier literature where the significant relationships between the relevant constructs will be confirmed. For data collection, a cross-sectional survey plan will be used to collect data from the Generation Y employees working in the 5-star hotels across India. This technique is appropriate for the descriptive and predictive functions associated with correlation research and for measuring the inter-relationship amongst several variables used in the study.

Findings
Pearson correlations was applied which exhibited a practically substantial positive relationship between the organisation’s TMP and POS. It was also found that the perceived supervisor support does not mediate the relationship between TMP and intention to quit. The results of this research also approve that employees’ perception regarding the organisation’s actions has direct consequence on their perception of support from their supervisors. The research also found essentially significant negative association between POS and the employee’s intention to quit, where high levels of POS is associated with a reduced employee’s intention to quit the hotel organisation.

Originality/value
This study confirmed a causal relationship amongst the relevant construct, i.e. perceived TMP, the POS, the supervisory support and the Generation Y’s intention to quit. It also provided an understanding for the management to comprehend upon the perceptions regarding TMP and support and how it influences an employee’s intent to leave the organisation in the Indian hotel industry.