bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence for metacognitive bias in perception of voluntary action
Authors: Charles Lucie , Chardin Camille , Haggard Patrick 
Published: September 2018

AbstractStudies of metacognition often measure confidence in perceptual decisions. Much less is known about metacognition of action, and specifically about how people estimate the success of their own actions. In the present study, we compare metacognitive abilities between voluntary actions, passive movements matched to those actions, and purely visual signals. Participants reported their confidence in judging whether a brief visual probe appeared ahead or behind of their finger during simple flexion/extension movement. The finger could be moved voluntarily, or could be moved passively by a robot replaying their own previous movements. In a third condition, participants did not move, but a visual cursor replayed their previous voluntary movements. Metacognitive sensitivity was comparable when judging active movements, during passive finger displacement and visual cursor reply. However, a progressive metacognitive bias towards overconfidence was found for passive and for voluntary movements. Taken together, our results may partly explain some of the peculiarities that arise when one judges one’s own actions.
Optimisation and validation of hydrogel-based brain tissue clearing shows uniform expansion across anatomical regions and spatial scales
Authors: Tyson Adam L. , Akhtar Ayesha , Andreae Laura C. 
Published: September 2018

ABSTRACTImaging of fixed tissue is routine in experimental neuroscience, but is limited by the depth of tissue that can be imaged using conventional methods. Optical clearing of brain tissue using hydrogel-based methods (e.g. CLARITY) allows imaging of large volumes of tissue and is rapidly becoming commonplace in the field. However, these methods suffer from a lack of standardised protocols and validation of the effect they have upon tissue morphology. We present a simple and reliable protocol for tissue clearing along with a quantitative assessment of the effect of tissue clearing upon morphology. Tissue clearing caused tissue swelling (compared to conventional methods), but this swelling was shown to be similar across spatial scales and the variation was within limits acceptable to the field. The results of many studies rely upon an assumption of uniformity in tissue swelling, and by demonstrating this quantitatively, research using these methods can be interpreted more reliably.
Tunneling nanotubes contribute to the stroma-mediated imatinib resistance of leukemic cells
Authors: Kolba Marta D. , Dudka Wioleta , Zaręba-Kozioł Monika , Kominek Agata , Ronchi Paolo , Turos Laura , Wlodarczyk Jakub , Schwab Yannick , Cysewski Dominik , Srpan Katja , Davis Daniel M. , Piwocka Katarzyna 
Published: September 2018

AbstractIntercellular communication within the bone marrow niche significantly influences leukemogenesis and the sensitivity of leukemic cells to therapy. Tunneling nanotubes (TNTs) are a novel mode of intercellular cross-talk. They are long, thin membranous protrusions that enable the direct transfer of various cargo between cells. Here we show that TNTs are formed between leukemic and bone marrow stromal cells. Fluorescence confocal microscopy with 3D reconstructions, correlative light-electron microscopy and electron tomography provided evidence that TNTs transfer cellular vesicles between cells. The quantitative analysis demonstrated that the stromal cells stimulate TNT-mediated vesicle transfer towards leukemic cells. Transfer of vesicular cargo from stromal cells correlated with increased resistance to anti-leukemic treatment. Moreover, specific sets of proteins with a potential role in survival and the drug response were transferred within these vesicles. Altogether, we found that TNTs are involved in the leukemia-stroma cross-talk and the stroma-mediated cytoprotection of leukemic cells. Our findings implicate TNT connections as a possible target for therapeutic interventions within the leukemia microenvironment to attenuate stroma-conferred protection.
Generation of human neural retina transcriptome atlas by single cell RNA sequencing
Authors: Lukowski Samuel W. , Lo Camden Y. , Sharov Alexei , Nguyen Quan H. , Fang Lyujie , Hung Sandy S.C. , Zhu Ling , Zhang Ting , Nguyen Tu , Senabouth Anne , Jabbari Jafar S. , Welby Emily , Sowden Jane C. , Waugh Hayley S. , Mackey Adrienne , Pollock Graeme , Lamb Trevor D. , Wang Peng-Yuan , Hewitt Alex W. , Gillies Mark , Powell Joseph E. , Wong Raymond C.B. 
Published: September 2018

SummaryThe retina is a highly specialized neural tissue that senses light and initiates image processing. Although the functional organisation of specific cells within the retina has been well-studied, the molecular profile of many cell types remains unclear in humans. To comprehensively profile cell types in the human retina, we performed single cell RNA-sequencing on 20,009 cells obtained post-mortem from three donors and compiled a reference transcriptome atlas. Using unsupervised clustering analysis, we identified 18 transcriptionally distinct clusters representing all known retinal cells: rod photoreceptors, cone photoreceptors, Müller glia cells, bipolar cells, amacrine cells, retinal ganglion cells, horizontal cells, retinal astrocytes and microglia. Notably, our data captured molecular profiles for healthy and early degenerating rod photoreceptors, and revealed a novel role of MALAT1 in putative rod degeneration. We also demonstrated the use of this retina transcriptome atlas to benchmark pluripotent stem cell-derived cone photoreceptors and an adult Müller glia cell line. This work provides an important reference with unprecedented insights into the transcriptional landscape of human retinal cells, which is fundamental to our understanding of retinal biology and disease.
Id4 eliminates the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells
Authors: Blomfield Isabelle , Rocamonde Brenda , del Mar Masdeu Maria , Mulugeta Eskeatnaf , Vaga Stefania , van den Berg Debbie L. C. , Huillard Emmanuelle , Guillemot François , Urbán Noelia 
Published: September 2018

SUMMARYQuiescence is essential for the long-term maintenance of adult stem cells and tissue homeostasis. However, how stem cells maintain quiescence is still poorly understood. Here we show that stem cells in the dentate gyrus of the adult hippocampus actively transcribe the pro-activation factor Ascl1 regardless of their activation state. We found that the inhibitor of DNA binding protein Id4 suppresses Ascl1 activity in neural stem cell cultures. Id4 sequesters Ascl1 heterodimerisation partner E47, promoting Ascl1 protein degradation and neural stem cell quiescence. Accordingly, elimination of Id4 from stem cells in the adult hippocampus results in abnormal accumulation of Ascl1 protein and premature stem cell activation. We also found that multiple signalling pathways converge on the regulation of Id4 to reduce the activity of hippocampal stem cells. Id4 therefore maintains quiescence of adult neural stem cells, in sharp contrast with its role of promoting the proliferation of embryonic neural progenitors.
An Alternative Framework for Fluorescence Correlation Spectroscopy
Authors: Jazani Sina , Sgouralis Ioannis , Shafraz Omer M. , Levitus Marcia , Sivasankar Sanjeevi , Pressé Steve 
Published: September 2018

ABSTRACTFluorescence correlation spectroscopy (FCS), is a flexible and widely used tool routinely exploited forin vivoandin vitroapplications. While FCS provides estimates of dynamical quantities, such as diffusion coefficients, it demands high signal to noise ratios and long time traces, typically in the minute range. In principle, the same information can be extracted fromµ-s long time traces; however, an appropriate analysis method is missing. To overcome these limitations, we adapt novel tools inspired by Bayesian non-parametrics, which starts from the direct analysis of the observed photon counts. With this approach, we are able to analyze time traces, which are too short to be analyzed by existing methods, including FCS. Our new analysis extends the capability of single molecule fluorescence confocal microscopy based approaches, to probe processes several orders of magnitude faster in time and permits a reduction of phototoxic effects on living samples induced by long periods of light exposure.
The second edge of ADHD: an advantage in motor learning and performance with task-irrelevant background vibratory noise
Authors: Korman Maria , Meir-Yalon Lian , Egbarieh Nebal , Karni Avi 
Published: September 2018

AbstractYoung adults with ADHD often gain less than expected from practice sessions well-suited for their peers. Here, we tested whether task-irrelevant, low-intensity vibratory stimulation (VtSt), suggested to modulate motor learning, may compensate for such learning deficits. Participants were given training, either with or without VtSt, on a sequence of finger opposition movements. Under VtSt, typical individuals had reduced overnight, consolidation phase, gains; performance partly recovering one week later. In contrast, participants with ADHD benefitted from VtSt both during the acquisition (online) and the overnight skill consolidation (offline) phases. One week later, both groups showed robust retention of the gains in performance, but when tested with background VtSt, individuals with ADHD outperformed their typical peers. We propose that ADHD can confer advantages in performance, learning and skill memory consolidation in specific ‘noisy’ conditions that adversely affect typical adults; we conjecture that the effects of VtSt are contingent on baseline arousal levels.
beditor: A computational workflow for designing libraries of guide RNAs for CRISPR-mediated base editing
Authors: Dandage Rohan , Després Philippe C , Yachie Nozomu , Landry Christian R 
Published: September 2018

ABSTRACTCRISPR-mediated base editors have opened unique avenues for scar-free genome-wide mutagenesis. Here, we describe a comprehensive computational workflow called beditor that can be broadly adapted for designing guide RNA libraries with a range of CRISPR-mediated base editors, PAM recognition sequences and genomes of many species. Additionally, in order to assist users in selecting the best sets of guide RNAs for their experiments, a priori estimates, called beditor scores are calculated. These beditor scores are intended to select guide RNAs that conform to requirements for optimal base editing: the editable base falls within maximum activity window of the CRISPR-mediated base editor and produces non-confounding mutational effects with minimal predicted off-target effects. We demonstrate the utility of the software by designing guide RNAs for base-editing to create or remove thousands of clinically important human disease mutations.
Infection-generated electric field in gut epithelium drives bidirectional migration of macrophages
Authors: Sun Yaohui , Reid Brian , Ferreira Fernando , Luxardi Guillaume , Ma Li , Lokken Kristen L. , Zhu Kan , Xu Gege , Sun Yuxin , Ryzhuk Volodymyr , Guo Betty P. , Lebrilla Carlito B. , Maverakis Emanual M. , Mogilner Alex , Zhao Min 
Published: September 2018

AbstractMany bacterial pathogens hijack macrophages to egress from the port of entry to the lymphatic/blood-stream, causing dissemination of life-threatening infections. However, the underlying mechanisms are not well understood. Here, we report thatSalmonellainfection generates directional electric fields (EF) in the follicle-associated epithelium of mouse cecum.In vitroapplication of an EF, mimicking the infection-generated electric field (IGEF), induces directional migration of primary mouse macrophages to the anode, which is reversed to the cathode uponSalmonellainfection. This infection-dependent directional switch is independent of theSalmonellapathogenicity island 1 (SPI-1) type III secretion system. The switch is accompanied by a reduction of sialic acids on glycosylated surface components during phagocytosis of bacteria, which is absent in macrophages challenged by microspheres. Moreover, enzymatic cleavage of terminally exposed sialic acids reduces macrophage surface negativity and severely impairs directional migration of macrophages in response to EF. Based on these findings, we propose that macrophages are attracted to the site of infection by a combination of chemotaxis and galvanotaxis; after phagocytosis of bacteria, surface electrical properties of the macrophage change, and galvanotaxis directs the cells away from the site of infection.AbbreviationsCFU, colony-forming unit; Con A, Concanavalin A; EF, electric field; FAE, follicle-associated epithelium; GNL, Galanthus Nivalis lectin; IGEF, infection-generated electric field; Ji, electric current density; MAL-2, Maackia Amurensis lectin II; MLN, mesenteric lymph node; MOI, multiplicity of infection; nMFI, normalized mean fluorescence intensity; RCA-1, Ricinus Communis Agglutinin I; SNA, Sambucus Nigra lectin;S. Typhimurium,Salmonella entericaserotype Typhimurium; SPI-1,Salmonellapathogenicity island 1; PDMS, polydimethylsiloxane; TEP, trans-epithelial potential difference; TLR, Toll-like receptors; WGEF, wound-generated electric field
How does open innovation affect firms’ innovative performance
Authors: Zhou Huiping , Yao Yanhong , Chen Huanhuan 
Published: September 2018

PurposeThis paper aims to explore the direct effects of open innovation (OI) on firms’ innovative performance, and to examine the moderating effects of knowledge attributes, including knowledge distance, knowledge embeddedness and partner opportunism on this relationship.Design/methodology/approachSurvey data of 247 samples from China were used to test the proposed model through hierarchical regression analysis.FindingsThe findings indicate that the dimensions of OI are positively related to innovative performance. The results also reveal that knowledge distance positively moderates the relationship between inbound OI and innovative performance, whereas knowledge embeddedness negatively affects that relationship. Knowledge embeddedness negatively affects the relationship between inbound OI and innovative performance, whereas knowledge distance positively moderates that relationship. Thus, a new finding is proposed that knowledge attributes could align effectively with specific OI type to achieve superior innovation outcomes. In addition, the empirical results suggest that partner opportunism plays a negative moderating role on the relationship between outbound OI and innovative performance.Originality/valueThe proposed view that a firm’s innovation outputs will be superior when its knowledge attributes effectively align with OI enriches studies of the OI context and expands the literature of both the resource-based view and the knowledge-based view. Furthermore, this study provides insights into how OI benefits can be influenced by external contexts from the perspective of partners’ opportunistic behaviour.
Chinese reverse M&A: the Wu Wei paradigm of post-M&A integration process
Authors: Sun Zhe 
Published: September 2018

PurposeThe purpose of this paper is to unpack the black box of post-merger and acquisition (M&A) integration of reverse M&A by Chinese multinational enterprises (MNEs).Design/methodology/approachThis research adopts multiple cases of Chinese reverse M&A. Data are collected using the approaches of in-depth interviews, storytelling and narratives.FindingsThis research identifies various antecedents underlying Chinese post-M&A integration, such as asymmetries in resources, capabilities, vision and status between Chinese MNEs and acquired firms. The post-M&A integration process of Chinese reverse M&A consists of a top-down effortless integration initiated by Chinese MNEs with both benefits and problems, and a bottom-up reverse integration conducted by acquired firms.Originality/valueBy linking the pre-M&A phase and the post-M&A phase, this research builds a new model of post-M&A integration of Chinese reverse M&A from an indigenous Wu Wei paradigm. The new model counterpoises extant literature, shifting from the task and efficiency-focussed view to the people and harmony-focussed view.
Alcopops: a global perspective on the new category of alcoholic beverage
Authors: Precieuse Kavota Mwendiwva , Kumar Vikas , Suri Sheenam , Gat Yogesh , Kumar Ashwani 
Published: September 2018

Purpose
The purpose of this paper is to explore the history, classification, regulation, the current market and consumer trends and health effects of alcopops.

Design/methodology/approach
The articles reviewed were selected based on the following key descriptors such as alcopop, history, classification, types, production and consumption trend, health benefits and adverse effect.

Findings
“Alcopop” is a collective term used to refer to flavored alcoholic beverages (FAB), also known as malt alcoholic beverage; ready-to-drink beverage (RTD); pre-packaged spirit or premium packaged spirit; high-strength (HS) pre-mixed beverage; pre-mixed caffeinated alcoholic beverage or alcoholic energy drink, etc. Alcopops were introduced into the market in 1990s. Starting with the introduction of FAB in the form of wine coolers, a large number of alcoholic beverages have been introduced. FAB are sweet, containing relatively low alcohol content and especially designed for the young drinkers also called the “entry-level” drinkers. They are popular among young and underage drinkers, teenage girls particularly, and the industries use packaging materials and marketing strategies that appear to target the youth. These products are now marketed globally, and their production, classification and marketing vary by country based on national regulatory restrictions. In countries such as USA and Australia, the industry represents that the products were malt beverages for regulatory purpose which were found to be false as other products were derived from distilled spirits. The product has no health benefits so far, the government need to reform their regulations and include new definitions of alcopops with available restrictions that would be practice at both national and state levels until and unless there have been another scientifically approved method of production through which the beverage could be beneficial for human consumption.

Originality/value
This is a unique and comprehensive review that will provide a brief overview of alcopops, i.e., a global perspective on the new category of alcoholic beverage.
Digital skin of the construction site
Authors: Edirisinghe Ruwini 
Published: September 2018

Purpose
The future construction site will be pervasive, context aware and embedded with intelligence. The purpose of this paper is to explore and define the concept of the digital skin of the future smart construction site.

Design/methodology/approach
The paper provides a systematic and hierarchical classification of 114 articles from both industry and academia on the digital skin concept and evaluates them. The hierarchical classification is based on application areas relevant to construction, such as augmented reality, building information model-based visualisation, labour tracking, supply chain tracking, safety management, mobile equipment tracking and schedule and progress monitoring. Evaluations of the research papers were conducted based on three pillars: validation of technological feasibility, onsite application and user acceptance testing.

Findings
Technologies learned about in the literature review enabled the envisaging of the pervasive construction site of the future. The paper presents scenarios for the future context-aware construction site, including the construction worker, construction procurement management and future real-time safety management systems.

Originality/value
Based on the gaps identified by the review in the body of knowledge and on a broader analysis of technology diffusion, the paper highlights the research challenges to be overcome in the advent of digital skin. The paper recommends that researchers follow a coherent process for smart technology design, development and implementation in order to achieve this vision for the construction industry.
Analytical and numerical approaches for Falkner–Skan flow of MHD Maxwell fluid using a non-Fourier heat flux model
Authors: Abbasbandy S. , Mustafa M. 
Published: September 2018

Purpose
This paper aims to describe the laminar flow of Maxwell fluid past a non-isothermal rigid plate with a stream wise pressure gradient. Heat transfer mechanism is analyzed in the context of non-Fourier heat conduction featuring thermal relaxation effects.

Design/methodology/approach
Flow field is permeated to uniform transverse magnetic field. The governing transport equations are changed to globally similar ordinary differential equations, which are tackled analytically by homotopy analysis technique. Homotopy analysis method-Padè approach is used to accelerate the convergence of homotopy solutions. Also, numerical approximations are made by means of shooting method coupled with fifth-order Runge-Kutta method.

Findings
The solutions predict that fluid relaxation time has a tendency to suppress the hydrodynamic boundary layer. Also, heat penetration depth reduces for increasing values of thermal relaxation time. The general trend of wall temperature gradient appears to be similar in Fourier and Cattaneo–Christov models.

Research limitations/implications
An important implication of current research is that the thermal relaxation time considerably alters the temperature and surface heat flux.

Originality/value
Current problem even in case of Newtonian fluid has not been attempted previously.
A reflection on survey research in hospitality
Authors: Dolnicar Sara 
Published: September 2018

Purpose
Survey research has developed to become the default empirical approach to answering research questions in the field of hospitality (and many other fields of research within the social sciences). This paper aims to reflect on the use of survey research in hospitality and offers recommendations for improvement.

Design/methodology/approach
First, known dangers to validity associated with survey research are discussed. Next, a sample of studies recently published in leading hospitality journals is assessed in view of these known dangers. Finally, recommendations are offered for editors, reviewers, readers and authors to mitigate the risk of drawing invalid conclusions based on survey research.

Findings
Survey research is very common in hospitality research and is used to investigate a wide range of research questions and constructs under study. The nature of constructs studied, the answer scales used and the nature of the samples point to a substantial risk to the validity of conclusions drawn.

Practical implications
A number of risk mitigation measures are proposed that can help authors minimise the risks to validity arising from known dangers associated with survey research. These same risk mitigation measures can be used by editors and reviewers in the assessment of manuscripts and by readers to evaluate the validity of conclusions drawn in already published work.

Originality/value
The value of this study lies in reflecting from a distance on how the survey research is conducted in the social sciences in general and in hospitality research in specific. The paper reveals that some routine approaches particularly prone to undermining the validity of conclusions may have been adopted and offers a few suggestions how this risk can be mitigated.