bluuebunny/crossref_metadata_2025_split huggingface dataset:
The prognostic value of immunoscore in patients with colorectal cancer: A systematic review and meta‐analysis
Authors: Sun Guorui , Dong Xiaoyuan , Tang Xiaolong , Qu Hui , Zhang Hao , Zhao Ensheng 
Published: December 2018

AbstractThe tumor immune infiltrate, as recently evaluated with the immunoscore methodology, has been reported to be related to colorectal cancer (CRC) progression. Nevertheless, results varied from different studies. A meta‐analysis was conducted to solve this problem. We collected data from included studies to evaluate the prognostic role of immunoscore in CRC patients on overall survival (OS) and disease‐free survival (DFS). MEDLINE, EMBASE, and Cochrane libraries were searched through 30 June 2018. Hazard ratio (HR) with 95% confidence intervals (95% CI) was pooled using a random‐effects model for OS and a fixed‐effects model for DFS. Finally, eight studies (involving 4689 CRC cases) were identified as eligible publications. The results of the meta‐analysis showed that low immunoscore was significantly correlated with poor OS (HR = 1.74, 95% CI: 1.43‐2.13) and DFS (HR = 1.82, 95% CI: 1.64‐2.03). The findings from most subgroup analyses were consistent with those from the overall analysis. The immunoscore could be a useful prognostic marker in patients with CRC. It is necessary to evaluate immunological markers in international multicenter studies.
Building hierarchical structures for 3D scenes based on normalized cut
Authors: Zhao Xi , Su Zhenqiang , Yang Xinyu 
Published: December 2018

AbstractThe growing number of 3D scene data available online brings in new challenges for scene retrieval, understanding, and synthesis. Traditional shape processing methods have difficulty to manage 3D scenes because such methods ignore the contextual information, that is, the spatial relationship between the objects or groups of objects, which plays a significant role in describing scenes. Therefore, a context‐aware representation is needed to deal with such a problem. In this paper, we propose a method to build scene hierarchies based on contextual information. Given a 3D scene, we first use the interaction bisector surface to measure the affinity between different objects/elements of the scene and then apply the normalized cut method to build a hierarchical structure for the whole scene. The resulting hierarchical structure contains not only the relationship between the individual objects but also the relationship between object groups, which provides much richer information of the scene compared with a flat structure that only describes the contacts or affinity between the individual objects. We test our method using several public databases and show that the resulting structure is more consistent with the ground truth. We also show that our method can be used for point cloud segmentation and outperforms previous methods.
RXXPEG motif of MERIT40 is required to maintain spindle structure and function through its interaction with Tankyrase1
Authors: Zheng Duo , Xie Wangqing , Li Li , Jiang Wenqi , Zou Yongdong , Chiang Chengyao , Shao Genze , Yan Kaowen 
Published: December 2018

AbstractDeubiquitinase BRISC complex plays important role in the maintenance of spindle structure and function; however, the underlying mechanism remains largely undefined. Here we demonstrated that MERIT40, a core component of BRISC complex, directly interacts with the RXXPEG motif in the ARC‐V domain of Tankyrase1(TNKS1). Mutation of the RXXPEG motif in the MERIT40 (R28A) disrupted its interaction with TNKS1. Consistent with these data, R28A mutant cells displayed multiple mitotic defects including aberrant spindle assembly and chromosome misalignment. These results support a critical role of RXXPEG motif of MERIT40 in BRISC‐mediated regulation of TNKS1 function during spindle assembly.
Enhanced Photoelectrocatalytic H2 Evolution over Two‐Dimensional MoS2 Nanosheets Loaded on Cu‐Doped CdS Nanorods
Authors: Tian Haoyang , Liang Jintao , Ma Xuli , Cao Lele , Hu Xueyan , Gao Mengting , Yang Huimin , Liang Zhenhai 
Published: December 2018

AbstractTransition‐metal ion doping and efficient decoration by using a co‐catalyst have been proven to effectively inhibit the recombination of photogenerated electron‐hole pairs and broaden the visible‐light response region. Here, we have constructed MoS2 nanosheets decorated on a copper‐doped CdS nanorod composite as a noble‐metal‐free photoelectrocatalyst. This kind of composite material was constructed through a simple solvothermal method. The morphology, structure, chemical states, photoelectrochemical properties, and other properties were tested by using a diverse range of analytical techniques. Owing to the Cu2+ doping and the excellent electron‐capturing ability of MoS2, 5 % MoS2/Cu−CdS (with 7 % Cu2+ doping in CdS) exhibits excellent hydrogen evolution reaction with a rate of 10.18 mmol h−1 g−1, which is about 48 times higher than that of pure CdS. A significant increase in the photoelectrochemical performance of the composite catalytic material benefits from the synergistic effect between CdS and MoS2 and fast interfacial charge transfer, owing to the Cu2+ doping. These findings provide a new thought for further research of the ion doping in photoelectrocatalytic field.
Kinetic modelling of alkyl ketene dimer hydrolysis during the storage and papermaking process
Authors: Yan Ning , Wan Xiao‐Fang , Hu Hui‐Chao , Chai Xin‐Sheng 
Published: December 2018

AbstractAn investigation of the hydrolysis of diluted alkyl ketene dimer (AKD) dispersion was carried out under different conditions from which a kinetic model for predicting the AKD hydrolysis during its storage and papermaking process was proposed. As observed, the higher pH, elevated temperature, and longer time can accelerate the hydrolysis of AKD in the water medium. The results showed that the proposed model could provide a satisfactory description of AKD hydrolysis at various conditions, in which the R‐squared of the correlation coefficient (R2) between the measured and predicted data was 0.978. Based on this model, a successful controlling strategy aimed at optimizing the chemical efficiency in the practical application can be determined.
The redox fountain
Authors: Ducci Matthias 
Published: December 2018

AbstractIm Beitrag werden spektakuläre Varianten des bekannten Springbrunnenversuchs vorgestellt. Während bei dem originären Experiment Säure‐Base‐Indikatoren eingesetzt werden, wird bei den folgenden Versuchen der sich verändernde pH‐Wert beim Einströmen der Lösung in den Kolben ausgenutzt, um Redoxreaktionen zu initiieren.
CNT‐Catalyzed Oxidative Dehydrogenation of Ethylbenzene to Styrene: DFT Calculations Disclose the Pathways
Authors: Marforio Tainah Dorina , Bottoni Andrea , Zerbetto Francesco , Calvaresi Matteo 
Published: December 2018

AbstractOxygen atoms, in the form of epoxy or carbonyl groups present at the edges of carbon nanotubes, trigger oxidative dehydrogenation of ethylbenzene to styrene. DFT calculations reveal that the process can occur along three pathways. The first two bifurcate from the initial transformation of an epoxide into a hydroxyl group, which occurs via a biradical transition state and the formation of a benzyl‐like intermediate. The epoxide can further react to release styrene and form a water molecule, as observed experimentally, via a highly exothermic process. Alternatively, in the presence of a second adjacent epoxide, styrene is also produced without water formation along a less exothermic pathway that leaves two hydroxyl groups on the nanotube surface. Along the third pathway, two adjacent carbonyl groups (quinone functionality) also promote the formation of styrene, with energy barriers similar to those calculated in the presence of epoxy groups. These are in the range 36–37 kcal mol−1. These values that can be easily surmounted at the working temperature used in the experiment (between 450 and 550 °C).
Implementation and Validation of an Automated Flow Cytometry Analysis Pipeline for Human Immune Profiling
Authors: Conrad Valerie K. , Dubay Christopher J. , Malek Mehrnoush , Brinkman Ryan R. , Koguchi Yoshinobu , Redmond William L. 
Published: December 2018

AbstractAutomated reagent preparation, sample processing, and data acquisition have increased the rate at which flow cytometry data can be generated. Furthermore, advances in technology and flow cytometry instrumentation continually increase the complexity and dimensionality of this data. Together, this leads to increased pressure on manual data analysis, which has inherent limitations including subjectivity of the analyst and the length of time needed for data processing. These issues can create bottlenecks in the data processing workflow and potentially compromise data quality. To address these issues, as well as the challenges associated with manual gating in a high‐volume human immune profiling laboratory, we sought to implement an automated analysis pipeline. In this report, we discuss considerations for selecting an automated analysis method, the process of implementing an automated pipeline, and detail our successful incorporation of an automated gating strategy with flowDensity into our analysis workflow. This validated pipeline augments our laboratory's ability to provide rapid high‐throughput immune profiling for patients participating in cancer immunotherapy clinical trials. © International Society for Advancement of Cytometry
Comparison of CerviHPV and Hybrid Capture 2 HPV tests for detection of high‐risk HPV infection in cervical swab specimens
Authors: Zhao Guodong , Tian Yaping , Du Yingying , Sun Jinli , Wang Zhenzhen , Ma Yong , Zheng Minxue 
Published: December 2018

BackgroundPersistent high‐risk human papillomavirus (HR‐HPV) infection is the etiological cause of virtually all cervical cancer cases. HR‐HPV screening achieved with earlier generations of HR‐HPV tests has been instrumental in the prevention and early detection of cervical cancer worldwide. The first FDA‐approved HR‐HPV test, digene Hybrid Capture 2 HPV DNA Test (HC2), has been prominent in these efforts. Newer tests have since been developed to improve upon the capability of HC2 test.MethodsTo evaluate the performance of a new multiplex real‐time quantitative PCR assay for HR‐HPV detection, CerviHPV HR‐HPV Test (CerviHPV), 232 cervical swab specimens were collected and analyzed by HC2 and CerviHPV tests for comparison.ResultsHC2 test detected 69 (29.7%) positive cases, whereas CerviHPV test reported 43 (18.5%) positive cases. The concordance rate between the two tests was 84.5% with a kappa value of 0.579. Additional analyses identified only HPV66 or low‐risk HPV (LR‐HPV) types in six HC2 positive discordant cases, suggesting these HC2 results to be false positive.ConclusionCerviHPV test has two advantages over HC2 test: It contains a cellular control to eliminate false negative results due to failed sample collection and processing, and it can simultaneously detect and genotype the two most carcinogenic HPV types, HPV16 and 18. In this comparison study, CerviHPV test also demonstrated higher analytical specificity for HR‐HPV genotypes than HC2 test. Therefore, CerviHPV test has the potential to become a viable option for cervical cancer screening in the clinics.
From feces to data: A metabarcoding method for analyzing consumed and available prey in a bird‐insect food web
Authors: Rytkönen Seppo , Vesterinen Eero J. , Westerduin Coen , Leviäkangas Tiina , Vatka Emma , Mutanen Marko , Välimäki Panu , Hukkanen Markku , Suokas Marko , Orell Markku 
Published: December 2018

AbstractDiets play a key role in understanding trophic interactions. Knowing the actual structure of food webs contributes greatly to our understanding of biodiversity and ecosystem functioning. The research of prey preferences of different predators requires knowledge not only of the prey consumed, but also of what is available. In this study, we applied DNA metabarcoding to analyze the diet of 4 bird species (willow tits Poecile montanus, Siberian tits Poecile cinctus, great tits Parus major and blue tits Cyanistes caeruleus) by using the feces of nestlings. The availability of their assumed prey (Lepidoptera) was determined from feces of larvae (frass) collected from the main foraging habitat, birch (Betula spp.) canopy. We identified 53 prey species from the nestling feces, of which 11 (21%) were also detected from the frass samples (eight lepidopterans). Approximately 80% of identified prey species in the nestling feces represented lepidopterans, which is in line with the earlier studies on the parids' diet. A subsequent laboratory experiment showed a threshold for fecal sample size and the barcoding success, suggesting that the smallest frass samples do not contain enough larval DNA to be detected by high‐throughput sequencing. To summarize, we apply metabarcoding for the first time in a combined approach to identify available prey (through frass) and consumed prey (via nestling feces), expanding the scope and precision for future dietary studies on insectivorous birds.
Trace element characterization of bitumen constraints on the hydrocarbon source of the giant gas field in Sichuan Basin, South China
Authors: Ni Zhiyong , Chen Zhonghong , Li Meijun , Yang Chengyu , Wen Long , Hong Haitao , Luo Bing 
Published: December 2018

The exploration of high production, natural gas wells (>106 m3/d) has been extremely successful in the Gaoshiti and Moxi areas of the Leshan–Longnvsi paleo‐uplift, in the west‐central Sichuan Basin. This paper focuses on the Sinian and Cambrian reservoirs from the Gaoshiti and Moxi areas, where a dataset of new trace elements in bitumen and potential source rock is reported and where hydrocarbon‐source correlation is attempted based on trace elements characteristics of the 12 potential source rocks and 22 bitumen samples. Results show that the bitumen from the three reservoirs can be distinguished from each other using trace element distribution patterns and redox‐ and provenance‐sensitive elemental ratios. Bitumen in the second and fourth members of the Sinian Dengying Formation were derived primarily from the third member of the source rocks of the Dengying Formation, which represents a self‐sourced paleo‐petroleum system. In contrast, bitumen in the reservoirs of the Cambrian Longwangmiao Formation is derived from the Cambrian Qiongzhusi Formation and third member of Dengying Formation. The trace element method used in this study improves our understanding of the complex hydrocarbon–source relation and confirm the potential target for petroleum exploration in the study area.
Capturing Preservice Chemistry Teachers’ Visual Representations of Redox Reactions through Storyboards
Authors: Akaygun Sevil , Adadan Emine , Kelly Resa 
Published: December 2018

AbstractThis study aimed to identify preservice chemistry teachers’ understanding of redox reactions at the submicroscopic level and how they make connections to macroscopic level. Twenty‐five preservice chemistry teachers first viewed a video of a redox reaction happening between a zinc wire and a copper(II) sulfate solution, then they reflected on their macroscopic observations, and generated storyboards (a sequence of drawings) representing this reaction at the submicroscopic level. Both the reflections and the storyboards were coded, and the categories reflecting the participants’ levels of understanding were determined. Results showed that the participants usually identified the macroscopic evidences, but they had conceptual difficulties in interpreting and representing evidences at the submicroscopic level. Few participants indicated a scientific or a moderate understanding of redox reactions, whereas the majority exhibited either weak or alternative understanding of redox reactions.
Medication adherence: Scope of the problem, ways to measure, ways to improve, and the role of the pharmacist
Authors: Touchette Daniel R. , Sharp Lisa K. 
Published: December 2018

BackgroundMedications are the cornerstone for the management of chronic conditions; however, approximately half of Americans do not take their prescribed medications as intended. Such behavior results in suboptimal clinical outcomes and financial strains on the health care system.AimsThis manuscript outlines challenges with addressing medication adherence and proposes an integrated conceptual model with opportunities for pharmacists to intervene.Discussion and conclusionPharmacists have many opportunities to support patients in adhering to their medications by addressing contextual issues.
Structural bioinformatics insights into ATP binding mechanism in zebrafish (Danio rerio) cyclin‐dependent kinase‐like 5 (zCDKL5) protein
Authors: Rout Ajaya Kumar , Mishra Jagruti , Dehury Budheswar , Maharana Jitendra , Acharya Varsha , Karna Subodha Kumar , Parida Pranaya Kumar , Behera Bijay Kumar , Das Basanta Kumar 
Published: December 2018

AbstractIn mammalian systems, the conserved cyclin‐dependent protein kinases (CDKs) control the process of cell division and curb the transcription mechanism in response to diverse signaling events that are essential for the catalytic activity. In zebrafish, zCDKL5 portrays differential expression profiling in several tissues and presumed to play a vital role in the neuronal development. In this present study, the sequence‐structure relationship and mode of ATP binding in zCDKL5 was unveiled through theoretical modeling, molecular docking, and MD simulations. Like human CDKs, the modeled zCDKL5 was found to be bipartite in nature, where, ATP binds to the central cavity of the catalytic domain through a strong network of H‐bonding, electrostatic, and hydrophobic interactions. MD simulation portrayed that conserved residues, viz, Ile10, Gly11, Glu12, Val18, Val64, Glu81, Cys143, and Asp144 were indispensable for tight anchoring of ATP and contribute to the stability of the zCDKL5‐ATP complex. MM/PBSA binding free energy analysis displayed that van der Waal energy (ΔG
vwd) and Electrostatic energy (ΔG
ele) were the major contributors towards the overall binding free energy. Thus, the comparative structural bioinformatics approach has shed new insights into the dynamics and ATP binding mechanism of zCDKL5. The results from the study will help to undertake further research on the role of phosphorylated CDKL5 in the onset of neurodevelopmental disorders caused by mutations in higher eukaryotic systems.
Retracted: Effects of ebv‐miR‐BART7 on tumorigenicity, metastasis, and TRAIL sensitivity of non‐small cell lung cancer
Authors: Wang Lei , Liu Li‐fa , Zhou Li , Liao Fei , Wang Ju 
Published: December 2018

AbstractObjectiveTo investigate how the Epstein‐Barr virus (EBV) encoded microRNA BART7 (miR‐BART7) affects tumorigenicity, metastasis, and TRAIL sensitivity of non‐small cell lung cancer (NSCLC).MethodsReal time‐polymerase chain reaction was performed to detect miR‐BART7 expression in NSCLC cell lines. A549 and Calu‐1 cells transfected with miR‐BART7 inhibitors/mimics were used to do the in‐vitro experiments, including 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, Annexin V‐fluorescein isothiocyanate/PI, wound‐healing, transwell, clonogenic assays, Western blot analysis, and anchorage‐independent growth assay. Additionally, mice were used to inject A549 cells infected with miR‐BART7 inhibitors to observe the tumorigenicity and metastasis of NSCLC.ResultsTRAIL‐resistant NSCLC cell lines (H460R, A549, Calu‐1, and H1299) exhibited higher miR‐BART7 rather than sensitive H460 and H292 cells. After transfected with miR‐BART7 inhibitors, we observed an inhibition in proliferation, migration, invasion, and colony formation, but an enhancement in apoptosis as well as expressions of caspase‐3 and caspase‐8 in A549 and Calu‐1 cells. Besides, TRAIL elevated the migration, invasion, and anchorage‐independent growth of A549 cells, which was reversed by silencing DR4 and DR5 (siDRs). However, miR‐BART7 inhibitors could reduce migration, invasion, and transformation potential of TRAIL treated A549 cells. Moreover, the expression of transforming growth factor‐beta 1 (TGFβ1) could be decreased by miR‐BART7 inhibitors with or without TRAIL treatment. Moreover, the tumor growth, epithelial‐to‐mesenchymal transition, and metastasis was suppressed and tumor‐free survival was extended after injection of A549‐miR‐BART7 inhibitors.ConclusionInhibition of miR‐BART7 exerted inhibitory effects on cell proliferation, migration, invasion, and colony formation, consequently facilitating cell apoptosis and raising TRAIL sensitivity, providing a new therapeutic target in NSCLC.
Sprouty1 regulates neuritogenesis and survival of cortical neurons
Authors: Gu Xi , Su Xiaohong , Jia Chunhong , Lin Lifang , Liu Shuhu , Zhang Peidong , Wang Xuemin , Jiang Xiaodan 
Published: December 2018

AbstractIn multicellular organisms, receptor tyrosine kinases (RTKs) control a variety of cellular processes, including cell proliferation, differentiation, migration, and survival. Sprouty (SPRY) proteins represent an important class of ligand‐inducible inhibitors of RTK‐dependent signaling pathways. Here, we investigated the role of SPRY1 in cells of the central nervous system (CNS). Expression of SPRY1 was substantially higher in neural stem cells than in cortical neurons and was increased during neuronal differentiation of cortical neurons. We found that SPRY1 was a direct target gene of the CNS‐specific microRNA, miR‐124 and miR‐132. In primary cultures of cortical neurons, the neurotrophic factors brain‐derived neurotrophic factor (BDNF) and Basic fibroblast growth factor (FGF2) downregulated SPRY1 expression to positively regulate their own functions. In immature cortical neurons and mouse N2A cells, we found that overexpression of SPRY1 inhibited neurite development, whereas knockdown of SPRY1 expression promoted neurite development. In mature neurons, overexpression of SPRY1 inhibited the prosurvival effects of both BDNF and FGF2 on glutamate‐mediated neuronal cell death. SPRY1 was also upregulated upon glutamate treatment in mature neurons and partially contributed to the cytotoxic effect of glutamate. Together, our results indicate that SPRY1 contributes to the regulation of CNS functions by influencing both neuronal differentiation under normal physiological processes and neuronal survival under pathological conditions.
Microfiltration membrane possessing chelation function and its adsorption and rejection properties towards heavy metal ions
Authors: Du Ruikui , Gao Baojiao , Men Jiying 
Published: December 2018

AbstractBackgroundRemoving toxic heavy metal ions from water bodies is one of the most important areas of water treatment, and using porous membranes with a chelating function is a good route for this. Graft‐type porous membranes with a chelating function are innovate functional membranes. In this work, the main aims are to prepare such a functional membrane and evaluate its adsorption and rejection properties for heavy metal ions in water through adsorption isotherm experiments and permeation experiments.ResultsGlycidyl methacrylate (GMA) was graft‐polymerized on the surface of polysulfone (PSF) microfiltration membrane through a surface initiating system, getting the grafted membrane PSF‐g‐PGMA. Then the ring‐opening reaction of the epoxy group of the grafted PGMA was carried out with iminodiacetic acid (IDAA) as reagent, obtaining PSF‐g‐PGMA/IDAA membrane, on which a great deal of IDAA as chelating ligand was bonded. PSF‐g‐PGMA/IDAA membrane possesses a strong adsorption ability towards heavy metal ions via the chelating action and electrostatic interaction. The order of adsorption capacity was Pb2+>Cu2+>Cd2+, and the adsorption capacity of Pb2+ ion reached 4.5 µmol cm−2. When an injection filter with a membrane area of 1.77 cm2 was used, the rejection rate of PSF‐g‐PGMA/IDAA membrane for the three heavy metal ions with concentrations of 0.1 mmol L−1 were maintained at a level of 95% until the permeation solution volume reached 100 mL.ConclusionThe grafted microfiltration membrane PSF‐g‐PGMA/IDAA can be easily prepared and possesses excellent rejection and removing performance towards heavy metal ions © 2018 Society of Chemical Industry