bluuebunny/crossref_metadata_2025_split huggingface dataset:
First record of the genus Punicodoxa (Acari: Microdispidae) from Asia, with description of a new species phoretic on termites (Insecta: Isoptera)
Authors: Azhari Shahrzad , Hajiqanbar Hamidreza , Talebi Ali Asghar 
Published: February 2018

A new species of the genus Punicodoxa Mahunka, 1978 (Acari: Prostigmata: Microdispidae) is described and illustrated from Southwestern Iran: Punicodoxa termitophila Azhari and Hajiqanbar sp. nov., discovered from a termite Anacanthotermes sp. (Isoptera: Hodotermitidae). This species is the second representative of the genus Punicodoxa which is the first record of the genus form Asia. Furthermore, phoretic association between mites of the family Microdispidae and termites of the family Hodotermitidae, and also phoresy on insects in the genus Punicodoxa are reported for the first time.
Metabolism, Excretion, and Mass Balance of Solithromycin in Humans
Authors: MacLauchlin Christopher , Schneider Stephen E. , Keedy Kara , Fernandes Prabhavathi , Jamieson Brian D. 
Published: February 2018

ABSTRACT

Solithromycin, a novel macrolide and the first fluoroketolide, is being developed as a therapy for community-acquired bacterial pneumonia, with a distinct mechanism that provides activity against macrolide-resistant bacteria. The pharmacokinetics, metabolism, and excretion of solithromycin were studied in healthy male subjects after oral administration of a single 800-mg (∼100-μCi) dose of [
14
C]solithromycin. Solithromycin was well tolerated, and absorption from the solution occurred with a median time to peak concentration of 4.0 h. Solithromycin and the total radioactivity had similar profiles with no long-lived metabolites. The whole-blood total radioactivity was approximately 75% of the plasma total radioactivity. Recovery was essentially complete (mean, 90.6%), with 76.5% and 14.1% of the dose recovered in feces and urine, respectively. Unchanged solithromycin (CEM-101) was the predominant circulating radioactive component in plasma (77% of the total radioactivity area under the concentration-time curve [AUC]), with two minor plasma metabolites, CEM-214 and CEM-122 (
N
-acetyl-CEM-101), each accounting for approximately 5% of the total radioactivity. Urinary excretion was predominantly like that of the parent. Solithromycin was primarily eliminated in the feces after extensive metabolism via a complex metabolic pathway with CEM-262 as the major constituent (27.36% of the administered dose). Overall oxidative pathways, presumably carried out mostly by CYP3A4, represented the majority of the metabolism, with
N
-acetylation present to a lesser extent. No disproportionate human metabolites were observed.
Genome Sequence of a Byssochlamys sp. Strain Isolated from Fouled B20 Biodiesel
Authors: Stamps Blake W. , Andrade Oderay C. , Lyon Wanda J. , Floyd James G. , Nunn Heather S. , Bojanowski Caitlin L. , Crookes-Goodson Wendy J. , Stevenson Bradley S. 
Published: February 2018

ABSTRACT

Byssochlamys
sp. strain AF001 is a filamentous fungus isolated from fouled B20 biodiesel. Its growth on B20 biodiesel results in the degradation and fouling of the fuel and higher rates of corrosion in affected storage tanks. The genome of
Byssochlamys
sp. AF001 is 35.9 Mbp and is composed of 10 scaffolds, with a G+C content of 45.89%.
Complete Genome Sequence of a Swiss Hepatitis E Virus Isolate from the Liver of a Fattening Pig
Authors: Wist Valerie , Kubacki Jakub , Lechmann Julia , Steck Marco , Fraefel Cornel , Stephan Roger , Bachofen Claudia 
Published: February 2018

ABSTRACT
We present here the full-length genome sequence of a hepatitis E genotype 3 virus (HEV-3) isolate, CH_VW117, from the liver of a healthy fattening pig collected at the slaughter level. Sequence analysis implies that this strain belongs to the newly proposed HEV subtype 3s.
Complete Genome Sequence of a Ciprofloxacin-Resistant Salmonella enterica subsp. enterica Serovar Kentucky Sequence Type 198 Strain, PU131, Isolated from a Human Patient in Washington State
Authors: Shah Devendra H. , Paul Narayan C. , Guard Jean 
Published: February 2018

ABSTRACT

Strains of the ciprofloxacin-resistant (Cip
r
)
Salmonella enterica
subsp.
enterica
serovar Kentucky sequence type 198 (ST198) have rapidly and extensively disseminated globally to become a major food safety and public health concern. Here, we report the complete genome sequence of a Cip
r
S.
Kentucky ST198 strain, PU131, isolated from a human patient in Washington State (USA).
Draft Genome Sequence of Sphingorhabdus sp. Strain EL138, a Metabolically Versatile Alphaproteobacterium Isolated from the Gorgonian Coral Eunicella labiata
Authors: Silva Sandra Godinho , Lago-Lestón Asunción , Costa Rodrigo , Keller-Costa Tina 
Published: February 2018

ABSTRACT

Here, we report the draft genome sequence of
Sphingorhabdus
sp. strain EL138, an alphaproteobacterium that shows potential to degrade polycyclic aromatic compounds and to cope with various heavy metals and antibiotics. Moreover, the strain, isolated from the gorgonian coral
Eunicella labiata
, possesses several genes involved in the biosynthesis of polyphosphates, polyketides, and terpenoids.
Draft Genome Sequence of Ezakiella peruensis Strain M6.X2, a Human Gut Gram-Positive Anaerobic Coccus
Authors: Diop Awa , Diop Khoudia , Tomei Enora , Raoult Didier , Fenollar Florence , Fournier Pierre-Edouard 
Published: February 2018

ABSTRACT

We report here the draft genome sequence of
Ezakiella peruensis
strain M6.X2
T
. The draft genome is 1,672,788 bp long and harbors 1,589 predicted protein-encoding genes, including 26 antibiotic resistance genes with 1 gene encoding vancomycin resistance. The genome also exhibits 1 clustered regularly interspaced short palindromic repeat region and 333 genes acquired by horizontal gene transfer.
Complete Genome Sequence of a
Pepper mild mottle virus
Isolate from Northeast China
Authors: Yu Man , Zhou Tao , Wu Yuanhua , An Mengnan 
Published: February 2018

ABSTRACT

The complete genome sequence of a
Pepper mild mottle virus
(PMMoV) isolate obtained from Northeast China was determined by reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). Phylogenetic analysis showed that the virus isolate is closely related to Japanese, Chinese, Spanish, and U.S. isolates.
Budget impact analysis of heparin-bonded polytetrafluoroethylene grafts (Propaten) against standard polytetrafluoroethylene grafts for below-the-knee bypass in patients with critical limb ischaemia in France
Authors: Vergnaud Simon , Riche Valéry-Pierre , Tessier Philippe , Mauduit Nicolas , Kaladji Adrien , Gouëffic Yann 
Published: February 2018

ObjectivesTo evaluate the budget impact of progressive replacement of standard polytetrafluoroethylene (PTFE) grafts by heparin-bound PTFE (Propaten) for below-the-knee (BTK) bypass in patients with critical limb ischaemia (CLI).DesignFrom a review of the scientific literature, we calculated a theoretical BTK primary patency for Propaten grafts. Using the French hospital expenditure database (PMSI), we retrospectively estimated a rehospitalisation rate for standard PTFE grafts. From these data, a model was created to assess the budget impact of a progressive replacement from standard PTFE grafts to Propaten grafts over a 5-year horizon. We performed an univariate sensitivity analysis to assess the robustness of our results.SettingFrench National Health Insurance (FNHI) perspective.ParticipantPatients with CLI.Main outcome measuresBudget impact analysis.ResultsData extraction from the PMSI revealed that 656 patients were treated with PTFE grafts in 2011 in French public hospitals for a BTK bypass. Assuming a 2-year survival rate of 76.8%, observed reinterventions rate for standard PTFE grafts at 24 months from the PMSI was 35.1%. The mean rehospitalisation cost was €10 689. The budget impact analysis based on these data found a net cumulative 5-year payer budget reduction of €112 420 in favour of Propaten, under the assumption of a 75.6% primary patency for Propaten grafts for a projected population of 3215 patients of which 801 received a Propaten graft.ConclusionsOur budget impact analysis showed a positive impact on the national health insurance budget of the replacement of standard PTFE grafts by Propaten grafts for BTK bypass in patients with CLI in France. This supports the enactment of a reimbursement policy by the FNHI.
Emergency department presentation and readmission after index psychiatric admission: a data linkage study
Authors: Li Xue , Srasuebkul Preeyaporn , Reppermund Simone , Trollor Julian 
Published: February 2018

ObjectiveTo use linked administrative datasets to assess factors associated with emergency department (ED) presentation and psychiatric readmission in three distinctive time intervals after the index psychiatric admission.DesignA retrospective data-linkage study.SettingCohort study using four linked government minimum datasets including acute hospital care from July 2005 to June 2012 in New South Wales, Australia.ParticipantsPeople who were alive and aged ≥18 years on 1 July 2005 and who had their index admission to a psychiatric ward from 1 July 2007 to 30 June 2010.Outcome measuresORs of factors associated with psychiatric admission and ED presentation were calculated for three intervals: 0–1 month, 2–5 months and 6–24 months after index separation.ResultsIndex admission was identified in 35 056 individuals (51% -males) with a median age of 42 years. A total of 12 826 (37%) individuals had at least one ED presentation in the 24 months after index admission. Of those, 3608 (28%) presented within 0–1 month, 6350 (50%) within 2–5 months and 10 294 (80%) within 6–24 months after index admission. A total of 14 153 (40%) individuals had at least one psychiatric readmission in the first 24 months. Of those, 6808 (48%) were admitted within 0–1 month, 6433 (45%) within 2–5 months and 7649 (54%) within 6–24 months after index admission. Principal diagnoses and length of stay at index admission, sociodemographic factors, Charlson Comorbidity Index score, drug and alcohol comorbidity, intellectual disability and other inpatient service use were significantly associated with ED presentations and psychiatric readmissions, and these relationships varied somewhat over the intervals studied.ConclusionSocial determinants of service use, drug and alcohol intervention, addressing needs of individuals with intellectual disability and recovery-oriented whole-person approaches at index admission are key areas for investment to improve trajectories after index admission.
Extent and cost of inappropriate use of tumour markers in patients with pulmonary disease: a multicentre retrospective study in Shanghai, China
Authors: Zhang Haichen , Song Yunxiao , Zhang Xiong , Hu Jun , Yuan Suwei , Ma Jin 
Published: February 2018

ObjectivesThe currently implemented healthcare reform in China requires substantial capital investment. Although overtreatment results in serious waste, inappropriate laboratory use is widespread, and overuse of tumour markers (TMs) has attracted increasing attention.DesignRetrospective study.SettingThe respiratory, thoracic surgery and oncology departments of three hospitals in Shanghai from 2014 to 2015.ParticipantsPatients with chronic obstructive pulmonary disease (COPD) and primary bronchogenic lung cancer (PLC). Based on clinical guidelines and physician experience, the criteria of suitability of TM examinations were determined, and the number, cost and proportion of inappropriate TM requests were analysed.ResultsThe area under the receiver operating characteristic curve for carcinoembryonic antigen+cytokeratin fragment 21-1+squamous cell carcinoma antigen+neuron-specific enolase in patients with COPD and PLC was 0.813, in accordance with the cost-effectiveness principle, indicating good clinical and health economics values. In the 2706 patients, 12 496–16 956 (58.27%–79.06%) of TM requests were inappropriate. Furthermore, the involved expense was 650 200–1 014 156 yuan, accounting for 7.69%–12.00% of examination expenses and 1.35%–2.11% of hospitalisation costs.ConclusionsWe found that the inappropriate use of TMs was widespread for patients with pulmonary disease. Clinicians should use TMs strictly according to the guidelines to effectively manage laboratory resources and control costs.
Empathy, burn-out and the use of gut feeling: a cross-sectional survey of Danish general practitioners
Authors: Pedersen Anette Fischer , Ingeman Mads Lind , Vedsted Peter 
Published: February 2018

ObjectiveResearch has suggested that physicians’ gut feelings are associated with parents’ concerns for the well-being of their children. Gut feeling is particularly important in diagnosis of serious low-incidence diseases in primary care. Therefore, the aim of this study was to examine whether empathy, that is, the ability to understand what another person is experiencing, relates to general practitioners’ (GPs) use of gut feelings. Since empathy is associated with burn-out, we also examined whether the hypothesised influence of empathy on gut feeling use is dependent on level of burn-out.DesignCross-sectional questionnaire survey. Participants completed the Jefferson Scale of Physician Empathy and The Maslach Burnout Inventory.SettingPrimary care.Participants588 active GPs in Central Denmark Region (response rate=70%).Primary outcome measuresSelf-reported use of gut feelings in clinical practice.ResultsGPs who scored in the highest quartile of the empathy scale had fourfold the odds of increased use of gut feelings compared with GPs in the lowest empathy quartile (OR 3.99, 95% CI 2.51 to 6.34) when adjusting for the influence of possible confounders. Burn-out was not statistically significantly associated with use of gut feelings (OR 1.29, 95% CI 0.90 to 1.83), and no significant interaction effects between empathy and burn-out were revealed.ConclusionsPhysician empathy, but not burn-out, was strongly associated with use of gut feelings in primary care. As preliminary results suggest that gut feelings have diagnostic value, these findings highlight the importance of incorporating empathy and interpersonal skills into medical training to increase sensitivity to patient concern and thereby increase the use and reliability of gut feeling.
How many patients could benefit from REBOA in prehospital care? A retrospective study of patients rescued by the doctors of the Paris fire brigade
Authors: Thabouillot Oscar , Bertho K , Rozenberg E , Roche N-C , Boddaert G , Jost D , Tourtier J-P 
Published: February 2018

IntroductionResuscitative endovascular balloon occlusion of the aorta (REBOA) is a technique to control haemorrhage by placing a retrograde catheter in an artery and inflating a balloon at its tip. This retrospective study aimed to evaluate the proportion of injured people who could potentially have benefited from this technique prior to hospitalisation, including on the scene or during transport.MethodsA retrospective analysis was conducted of all patients with trauma registered in the Paris Fire Brigade emergency medical system between 1 January and 31 December 2014. Inclusion criteria included all patients over 18 years of age with bleeding of supposedly abdominal and/or pelvic and/or junctional origin, uncontrolled haemorrhagic shock or cardiac arrest with attempted resuscitation.ResultsDuring this study period, a total of 1159 patients with trauma (3.2%) would have been eligible to undergo REBOA. Death on scene rate was 83.8% (n=31) and six patients had a beating heart when they arrived at the hospital. Ten out of the 37 patients had spontaneous circulatory activity. Among them, four people died on the scene or during transport. Thirty-six out of 37 patients were intubated, one benefited from the use of a haemostatic dressing and one benefited from a tourniquet.ConclusionsREBOA can be seen as an effective non-surgical solution to ensure complete haemostasis during the prehospital setting. When comparing the high mortality rate following haemorrhage with the REBOA’s rare side effects, the risk–benefit balance is positive. Given that 3% of all patients with trauma based on this study would have been eligible for REBOA, we believe that this intervention should be available in the prehospital setting. The results of this study will be used: educational models for REBOA balloon placement using training manikins, with an ultimate aim to undertake a prospective feasibility study in the prehospital setting.
The application of distributed optical strain sensing to measure the strain distribution of ground support members
Authors: Forbes Bradley , Vlachopoulos Nicholas , Hyett Andrew J. 
Published: February 2018

A distributed optical strain-sensing technique is presented as a solution for measuring the strain distribution along ground support members used in tunnelling and mining works. The technique employs a Rayleigh optical frequency domain reflectometry technology, which measures strain at a spatial resolution of 0.65 mm along the length of a standard optical fiber. A rationale for selecting this technology as a potential monitoring technique for ground support elements over alternative commercially available technologies is discussed. The development of a technique to couple optical fiber sensors with rock bolt, umbrella arch, and cable bolt support members is also demonstrated. A robust laboratory investigation of such optically instrumented support members demonstrated the capability of the technique to capture the expected in situ support behaviour in the form of coaxial, lateral, and shear loading arrangements as would be anticipated in the field. Moreover, the micro-scale data obtained by this optical sensing technique are shown to provide unprecedented insight into the local/micro-scale geomechanistic complexities associated with the bearing capacity of ground support members, especially when compared with data obtained by discrete strain-sensing technologies.
Computer-aided neurophysiology and imaging with open-source PhysImage
Authors: Hayes John A. , Papagiakoumou Eirini , Ruffault Pierre-Louis , Emiliani Valentina , Fortin Gilles 
Published: February 2018

Improved integration between imaging and electrophysiological data has become increasingly critical for rapid interpretation and intervention as approaches have advanced in recent years. Here, we present PhysImage, a fork of the popular public-domain ImageJ that provides a platform for working with these disparate sources of data, and we illustrate its utility using in vitro preparations from murine embryonic and neonatal tissue. PhysImage expands ImageJ’s core features beyond an imaging program by facilitating integration, analyses, and display of 2D waveform data, among other new features. Together, with the Micro-Manager plugin for image acquisition, PhysImage substantially improves on closed-source or blended approaches to analyses and interpretation, and it furthermore aids post hoc automated analysis of physiological data when needed as we demonstrate here. Developing a high-throughput approach to neurophysiological analyses has been a major challenge for neurophysiology as a whole despite data analytics methods advancing rapidly in other areas of neuroscience, biology, and especially genomics. NEW & NOTEWORTHY High-throughput analyses of both concurrent electrophysiological and imaging recordings has been a major challenge in neurophysiology. We submit an open-source solution that may be able to alleviate, or at least reduce, many of these concerns by providing an institutionally proven mechanism (i.e., ImageJ) with the added benefits of open-source Python scripting of PhysImage data that eases the workmanship of 2D trace data, which includes electrophysiological data. Together, with the ability to autogenerate prototypical figures shows this technology is a noteworthy advance.
Spatiotemporal evolution of focal epileptiform activity from surface and laminar field recordings in cat neocortex
Authors: Bink Hank , Sedigh-Sarvestani Madineh , Fernandez-Lamo Ivan , Kini Lohith , Ung Hoameng , Kuzum Duygu , Vitale Flavia , Litt Brian , Contreras Diego 
Published: February 2018

New devices that use targeted electrical stimulation to treat refractory localization-related epilepsy have shown great promise, although it is not well known which targets most effectively prevent the initiation and spread of seizures. To better understand how the brain transitions from healthy to seizing on a local scale, we induced focal epileptiform activity in the visual cortex of five anesthetized cats with local application of the GABAA blocker picrotoxin while simultaneously recording local field potentials on a high-resolution electrocorticography array and laminar depth probes. Epileptiform activity appeared in the form of isolated events, revealing a consistent temporal pattern of ictogenesis across animals with interictal events consistently preceding the appearance of seizures. Based on the number of spikes per event, there was a natural separation between seizures and shorter interictal events. Two distinct spatial regions were seen: an epileptic focus that grew in size as activity progressed, and an inhibitory surround that exhibited a distinct relationship with the focus both on the surface and in the depth of the cortex. Epileptiform activity in the cortical laminae was seen concomitant with activity on the surface. Focus spikes appeared earlier on electrodes deeper in the cortex, suggesting that deep cortical layers may be integral to recruiting healthy tissue into the epileptic network and could be a promising target for interventional devices. Our study may inform more effective therapies to prevent seizure generation and spread in localization-related epilepsies. NEW & NOTEWORTHY We induced local epileptiform activity and recorded continuous, high-resolution local field potentials from the surface and depth of the visual cortex in anesthetized cats. Our results reveal a consistent pattern of ictogenesis, characterize the spatial spread of the epileptic focus and its relationship with the inhibitory surround, and show that focus activity within events appears earliest in deeper cortical layers. These findings have potential implications for the monitoring and treatment of refractory epilepsy.