bluuebunny/crossref_metadata_2025_split huggingface dataset:
Umbilical mesenchymal stem cells attenuates corneal fibrosis induced by fungal keratitis through the TGFβ1/Smad2 signaling pathway
Authors: Zhou Yue , Chen Yuqing , Wang Suiyue , Qin Fangyuan , liya Wang 
Published: January 2019

Abstract
Background: Fungal keratitis (FK) is eye microbial infection disease which can leads to severe corneal blindness. Corneal scar formation is one of the major complications of fungal keratitis and is closely related to prognosis. The aim of the current investigation was to evaluate the anti-fibrosis effect of human umbilical cord mesenchymal stem cells (uMSCs) in FK model and further to explore underlying mechanisms.
Methods: FK mice model was made by corneal epithelial scratching and F. solani inoculation. The C57BL/6J mice were randomly divided into four groups, including control group, FK group, vehicleinj FK group and uMSCsinj FK group. After injury, antifungal drug natamycin eye drops were used topically to FK mice eyes 6 times per day for 7 days to inhibit fungi growth. Mice received repeated subconjunctival injection of uMSCs or veichle for 3 times including the 1d, 4d and 7d after wounding. At 14d, 21d and 28d post-injury, clinical observation, histological examination, second harmonic generation, immunofluoresence staining and molecular assays were performed.
Results: The uMSCs topical administration reduced corneal scar formation and corneal opacity, accompanying with decreased corneal thickness and inflammatory cell infiltration, following down-regulated fibrotic-related factors α-SMA, TGFβ1, CTGF, and COLⅠ and finally inhibited phosphorylation of TGFβ1/Smad2 signaling pathway, which indicating the potential anti-fibrotic and protective effect of human uMSCs against FK-induced corneal fibrosis.
Conclusion: Human uMSCs can evidently inhibit corneal fibrosis after FK wounding through TGFβ1/Smad2 signaling pathway regulation.
Keywords: Fungal keratitis, corneal fibrosis, umbilical cord mesenchymal stem cells
The Effects of Low versus Standard Pressure Pneumoperitoneum on Renal Tubular Epithelial and Peritubular Endothelial Cells Injury in Living-Donor Nephrectomy: A Randomized Controlled Study
Authors: Aditianingsih Dita , Mochtar Chaidir Arif , Lydia Aida , Siregar Nuryati Chairani , Margyaningsih Nur Ita , Madjid Amir Sjarifuddin , Suwarto Suhendro 
Published: January 2019

Abstract
Background: Laparoscopic nephrectomy is a preferable technique for living kidney donation. However, positive-pressure pneumoperitoneum may have an unfavorable effect on the remaining kidney and other distant organs due to increased systemic inflammation, endothelial vascular response and renal tubular injury. Early detection of renal injury due to increased intraabdominal pressure is needed. The aim of this study was to evaluate whether using low-pressure pneumoperitoneum reduces syndecan-1 shedding and soluble vascular endothelial growth factor receptor-2 (sVEGFR-2) expression as the early markers of vascular endothelial and renal tubular cell injury, in comparison to the standard pressure.
Methods: We conducted a prospective randomized study on 44 patients undergoing laparoscopic donor nephrectomy that were allocated into standard pressure (12 mmHg) group or low-pressure (8 mmHg) group. The serial plasma interleukin-6, syndecan-1, and sVEGFR-2 were collected at baseline, intra- and postoperative, and were quantified by ELISA. We analyzed syndecan-1 and VEGFR-2 expression from renal cortex tissue by immunohistochemistry and examined the ultrastructure of renal tubules and peritubular capillaries using electron microscopy. The perioperative hemodynamic, end-tidal CO2, serum creatinine, blood urea nitrogen and urinary KIM-1 were recorded.
Results: Forty-four patients were analyzed, the patients’ baseline and demographic characteristics were compared between groups. The low-pressure group showed significantly lower intra- and postoperative heart rate, intraoperative plasma IL-6 and sVEGFR-2 level compared to the standard pressure group. Plasma syndecan-1 level was lower in the low-pressure group although insignificant. Syndecan-1 expression in proximal tubules was significantly higher in the low-pressure group. The expression of VEGFR-2 in proximal, distal tubules and peritubular capillaries endothelium were significantly lower in the low-pressure group. The low-pressure group showed better morphological ultratructure of renal tubules and peritubular capillaries.
Conclusion: The low-pressure pneumoperitoneum reduced the inflammatory response of plasma IL-6, the shedding of syndecan-1 and VEGFR-2 expression as the marker for renal tubular injury in laparoscopic nephrectomy.
Trial registration: ClinicalTrial.gov NCT:03219398 on July 17th 2017.
Keywords: Pneumoperitoneum pressure, renal resistive index, interleukin-6, syndecan-1, VEGFR-2, laparoscopic nephrectomy
Clinico-pharmacological characteristics of opioid drug-drug interactions in cancer patients with chronic pain syndrome
Authors: Bobrova O. P. , Shnayder N. A. , Zyryanov S. K. , Dyhno Yu. A. , Petrova M. M. , Nasyrova R. F. 
Published: January 2019

The aimof the review was to analyze published studies on the impact of opioid drug-drug interactions on the choice of analgesic therapy regimens.Material and methods.A systematic literature search was conducted using PubMed, Scopus, Web of Science, and E-library databases.Results.The review showed a clinical significance of pharmacokinetic interactions of opioids with other drugs in cancer pain treatment. The problems of individual choice of analgesics from different groups under conditions of co-morbidity and concomitant medication were discussed to ensure the effectiveness/safety of the treatment strategy affecting the quality of life of cancer patients.Conclusion.A comprehensive assessment of factors in patients receiving opioid analgesics is a predictor of effective and safe analgesic therapy.
Computer tomographic semiotics of retroperitoneal leiomyosarcoma arising from the muscular wall of the splenic vein: a case report
Authors: Lagkueva I. D. , Rebrikova V. A. , Egorova E. V. , Sergeev N. L. , Kotlyarov P. M. , Bliznyukov O. P. 
Published: January 2019

Background. Diagnostic imaging of retroperitoneal neoplasms that arise within the retroperitoneal space but outside the major organs in this space is challenging due to the presence of large spaces filled with fatty tissue that allows the tumor to develop asymptomatically for a long time. In addition, these tumors are rare, and radiological semiotics and clinical manifestations can be nonspecific.Case presentation. We present the rare clinical case of a 53-year-old patient with a large retroperitoneal mass detected by contrast-enhanced computed tomography. Additional imaging diagnostic procedures as well as histological findings helped to make a definitive diagnosis: extraorganic retroperitoneal leiomyosarcoma arising from the muscular wall of the splenic vein.Conclusion. The retrospective analysis of CT data showed that the tumor-induced marginal defect in the contrasted splenic vein may indicate the tumor arising from the muscular wall of the vessel. The final diagnosis was made on the basis of a morphological study.
Characteristics of inflammatory reactions in the tumor microenvironment of head and neck squamous cell carcinoma
Authors: Bychkov V. A. , Bondar L. N. , Tashireva L. A. , Cheremisina O. V. , Choynzonov E. L. , Perelmuter V. M. 
Published: January 2019

Objective: to analyze the association between inflammatory cells with morphological heterogeneity and lymphogenous metastasis of head and neck squamous cell carcinoma.Material and Methods. Five types of tumor structures, the level of inflammatory infiltration and the counts of CD4+ and CD8+ t-lymphocytes, B-lymphocytes and macrophages in the stromal infiltrate and Langerhans cells in tumor structures were assessed in 44 patients.Results. The presence of Langerhans cells in type 1 structures was correlated with the presence of CD3+ and CD4+ t-cells in the inflammatory infiltrate. There was a decrease in the inflammatory reaction and the number of CD3+ T cells in patients with metastatic lymph nodes compared with patients with intact lymph nodes. In patients with lymph node metastasis, the proportion of CD4+ and / or CD8+ and / or CD20+ lymphocytes in the infiltrate was higher in cases with well-differentiated tumor than in cases with poorly-differentiated tumor.Conclusion. The results obtained showed the decrease in the level of inflammatory response, including t-cell response, as well as the relationship between the presence of Langerhans cells and stromal inflammatory response in patients with lymph node metastasis.
Chronotherapy in pediatrics is the basis for improving the effectiveness of treatment of children`s diseases
Authors: Neudakhin E. V. 
Published: January 2019

The article briefly describes the general ideas on the body’s biorhythms determining the essence of its homeostasis. The article pays special attention to the importance of alternative interaction of the body's reactions in the formation of biorhythms. It is emphasized that the violation of biorhythms (desynchronosis) is the most profound and most sensitive marker of functional disorders. In this regard, the practicing doctors should use methods of chronodiagnosis and chronotherapy more widely. The article presents the authors’ data and the data of other authors on the high efficiency of chronotherapy. This method, focused on the individual approach to the treatment of a child, makes it possible to increase the effectiveness of treatment while decreasing usage of drugs. The future of medicine is impossible without a chronobiological understanding of its problems.Conflict of interest: The author of this article confirmed the lack of conflict of interest and financial support, which should be reported.
Rare mutation of the CCNO gene in patients with primary ciliary dyskinesia
Authors: Bogorad A. E. , Dyakova S. E. , Mizernitskiy Yu. L. , Sokolova L. V. , Zakharov P. P. , Zorina I. E. , Kostyuchenko M. V. , Shatokha P. A. 
Published: January 2019

Primary ciliary dyskinesia is a rare genetically determined pathology leading to the development of chronic inflammatory lesions of the respiratory system in children, impaired fertile function in older patients. The disease is characterized by an autosomal recessive mode of inheritance with marked genetic heterogeneity. The article describes clinical observation of a patient – carrier of a rare mutation and describes the features of this case.Conflict of interest: The authors of this article confirmed the lack of conflict of interest and financial support, which should be reported.
Comparative study of conceptual versus distributed hydrologic modelling to evaluate the impact of climate change on future runoff in unregulated catchments
Authors: Al-Safi Hashim Isam Jameel , Kazemi Hamideh , Sarukkalige P. Ranjan 
Published: January 2019

Abstract
The application of two distinctively different hydrologic models, (conceptual-HBV) and (distributed-BTOPMC), was compared to simulate the future runoff across three unregulated catchments of the Australian Hydrologic Reference Stations (HRSs), namely Harvey catchment in WA, and Beardy and Goulburn catchments in NSW. These catchments have experienced significant runoff reduction during the last decades due to climate change and human activities. The Budyko-elasticity method was employed to assign the influences of human activities and climate change on runoff variations. After estimating the contribution of climate change in runoff reduction from the past runoff regime, the downscaled future climate signals from a multi-model ensemble of eight global climate models (GCMs) of the Coupled Model Inter-comparison Project phase-5 (CMIP5) under the Representative Concentration Pathway (RCP) 4.5 and RCP 8.5 scenarios were used to simulate the future daily runoff at the three HRSs for the mid-(2046–2065) and late-(2080–2099) 21st-century. Results show that the conceptual model performs better than the distributed model in capturing the observed streamflow across the three contributing catchments. The performance of the models was relatively compatible in the overall direction of future streamflow change, regardless of the magnitude, and incompatible regarding the change in the direction of high and low flows for both future climate scenarios. Both models predicted a decline in wet and dry season's streamflow across the three catchments.
Economic feasibility for selecting wastewater treatment systems
Authors: Goffi Andreia Santos , Trojan Flavio , de Lima José Donizetti , Lizot Mauro , Thesari Shirley Suellen 
Published: January 2019

Abstract
The ideal configuration of wastewater treatment system (WTS) for attending cities specificities has become a complex decision, due to the fact that there are several available technologies, and a diversity of characteristics presented in the scenario of each city. Considering the importance of economic analysis, especially in developing countries, this work aims to demonstrate the economic feasibility considering cost-related indicators for the ideal WTS selection for specific features in these cities. Based on a literature review, 37 main WTS and two economic cost-related indicators (Net Present Value and Annualized Net Present Value) were considered. First of all, using a multi-criteria analysis these WTS were grouped in classes using the ELECTRE TRI method, based on criteria related to efficiency, and the weights were defined by appointments from research specialists in the literature appointments. The economic analysis was performed using the Monte Carlo Simulation (MCS) method, which has been applied specifically to each WTS class, thus generating a framework of economic viability for this context. The WTS with low and high costs were appointed, considering the development level in each applied scenario. This work contributes to expanding the WTS study horizons to select an ideal system, considering the economic aspect.
Vanadium and Oxidative Stress Markers - In Vivo Model: A Review
Authors: Ścibior Agnieszka , Kurus Joanna 
Published: January 2019

:This review article is an attempt to summarize the current state of knowledge of the impact of Vanadium (V) on Oxidative Stress (OS) markers in vivo. It shows the results of our studies and studies conducted by other researchers on the influence of different V compounds on the level of selected Reactive Oxygen Species (ROS)/Free Radicals (FRs), markers of Lipid peroxidation (LPO), as well as enzymatic and non-enzymatic antioxidants. It also presents the impact of ROS/peroxides on the activity of antioxidant enzymes modulated by V and illustrates the mechanisms of the inactivation thereof caused by this metal and reactive oxygen metabolites. It also focuses on the mechanisms of interaction of V with some nonenzymatic compounds of the antioxidative system. Furthermore, we review the routes of generation of oxygen-derived FRs and non-radical oxygen derivatives (in which V is involved) as well as the consequences of FR-mediated LPO (induced by this metal) together with the negative/ positive effects of LPO products. A brief description of the localization and function of some antioxidant enzymes and low-molecular-weight antioxidants, which are able to form complexes with V and play a crucial role in the metabolism of this element, is presented as well. The report also shows the OS historical background and OS markers (determined in animals under V treatment) on a timeline, collects data on interactions of V with one of the elements with antioxidant potential, and highlights the necessity and desirability of conducting studies of mutual interactions between V and antioxidant elements.
Three-Dimensional (3-D) Printing Technology Exploited for the Fabrication of Drug Delivery Systems
Authors: Zeeshan Farrukh , Madheswaran Thiagarajan , Pandey Manisha , Gorain Bapi 
Published: January 2019

Background:The conventional dosage forms cannot be administered to all patients because of interindividual variability found among people of different race coupled with different metabolism and cultural necessities. Therefore, to address this global issue there is a growing focus on the fabrication of new drug delivery systems customised to individual needs. Medicinal products printed using 3-D technology are transforming the current medicine business to a plausible alternative of conventional medicines.Methods:The PubMed database and Google scholar were browsed by keywords of 3-D printing, drug delivery, and personalised medicine. The data about techniques employed in the manufacturing of 3-D printed medicines and the application of 3-D printing technology in the fabrication of individualised medicine were collected, analysed and discussed.Results:Numerous techniques can fabricate 3-D printed medicines however, printing-based inkjet, nozzle-based deposition and laser-based writing systems are the most popular 3-D printing methods which have been employed successfully in the development of tablets, polypills, implants, solutions, nanoparticles, targeted and topical dug delivery. In addition, the approval of Spritam® containing levetiracetam by FDA as the primary 3-D printed drug product has boosted its importance. However, some drawbacks such as suitability of manufacturing techniques and the available excipients for 3-D printing need to be addressed to ensure simple, feasible, reliable and reproducible 3-D printed fabrication.Conclusion:3-D printing is a revolutionary in pharmaceutical technology to cater the present and future needs of individualised medicines. Nonetheless, more investigations are required on its manufacturing aspects in terms cost effectiveness, reproducibility and bio-equivalence.
Loop Transverse Colostomy - A Modified Technique
Authors: Cai Cheng , Dai Zhihui , Zhong Zhifeng , Wang Jianping , Du Jinlin 
Published: January 2019

Background:
Transverse colostomy is commonly performed to create temporary stoma in
rectal cancer patients after neoadjuvant chemoradiotherapy. Conventional methods are either
difficult to implement or to care for. To resolve these problems, we herein describe a modified
transverse colostomy method.

Material and Methods:
Two sutures of peritoneum were made as “bridges” to support the stoma.
Absorbable sutures were utilized to reinforce the stoma. Once the stoma was created, the stoma bag
was immediately placed on the skin. 120 patients who received conventional or modified transverse
colostomy between 2008 and 2014 were selected. Then, the two groups of patients were compared
for stoma-related complications.

Results:
The operation time of stoma construction was 34±10 minutes for the conventional method
and 28±7 minutes for the modified method (P= 0.009). There were no significant differences
between the two groups with respect to postoperative bleeding, bowel obstruction or stoma
retraction. Patients with conventional transverse colostomy were remarkably more likely to
experience parastoma hernia (P= 0.048) and stoma prolapse (P= 0.038).

Conclusion:
In comparison with conventional methods, the modified transverse colostomy is a safe
and effective diverting technique. It can be readily performed by all kinds of surgeons, especially
those in underdeveloped areas. The technique represents a preferred method for constructing
temporary stoma in rectal cancer patients treated with neoadjuvant chemoradiotherapy.
Initial Study of Thoicyianate Microbial Degradation by Isolates from Poluted Soil in Gold Mining Area in Indonesia
Authors: Rakam Maman Rahmansyah 
Published: January 2019

This study was conducted to clarify the ability of denitrifying bacterial group utilized nitrogen (N) due to decompose N in thiocyanate structure.Thiocyanate is a chemical that has likely pollutant to the environment, produced by some industrial activities. Denitrifying bacterial group obtained from bulk of sluge samples collected from the gold tailing, and some soil samples collected suround the gold mining site. The samples then were taken to the Microbiology Laboratory, Research Center for Biology, to investigate. Samples were initially acclimatized by potassium nitrate (KNO3), acetonitrile, and liquid waste or sludge. The result showed that denitrifying bacteria in the samples utilize 60 to 90% NO3-N (nitrate) in 42 days incubation. Isolation process were then conducted in each samples, and four denitrification bacterial, named as AN, Ea, L7T5, and PETI-7 isolates were attained. The isolates formerly cultured in a denitrifying bacterial medium containing KSCN (Potassium Thiocyanate), amended with glucose and sodium acetate for carbon source. Those four isolates performed satisfactory in aerobic and anaerobic cultures medium to denitrifying process, and utilize glucose and sodium acetate as co-carbon source, but all bacterial isolates were unable to use thiocyanate as the single carbon source. Thiocyanate degradation performed by the isolates through a simultaneous conversion along with denitrification process. This phenomenon turn to open the opportunity on role of application denitrifying bacteria become bioresources material in efforts to decompost thiocyanate.
High Performance of Enzymatic Bioprocess for Production of Biomassed-based Bioethanol of Sago Palm Fiber Waste
Authors: Harihastuti Nani , Rame Rame , Djayanti Silvy 
Published: January 2019

Biomass waste in the form of fiber dregs contains many components of lignocellulose and hemicellulose. Lignocellulose can be used to produce ethanol through enzymatic biotechnology processes. Sago palm fiber industry is one potential industry producing biomass waste in the form of solid waste of fiber dregs (about 30% from the weight of processed raw materials). Solid fiber waste contains crude fiber and lignocellulose compound consists of cellulose (35-50%), hemicellulose ( 20-35%) and lignin (12-20%). This study aimed to utilize solid waste of sago palm fiber as a raw material of bioethanol production through enzymatic biotechnology processes of delignification, saccharification and fermentation which was then purified by distillation process to get ethanol. Delignification, saccharification, and fermentation stages are conducted using Phanerochaete chrysosporus Mushroom, Trichoderma viride fungus, and saccharomyces cerevisiae, respectively and then purified by distillation process (one level) to produce ethanol. The process of saccharification and fermentation process were done in an integrated manner (addition of Trichoderma viride and yeast saccharomyces cereviceae fungi done simultaneously). The weight of raw materials of solid waste fiber treated was about 6 kgs. Alcohol content obtained was about ± 4% (distillation). No water, air, and soil pollution inflicted, more added value, and sustainable were the main benefits of biotech process or bioprocess.
Planificación urbana y la influencia de las urbanizaciones cerradas: El Área Metropolitana de Guadalajara, México
Authors: Pfannenstein Bernd , Martínes Jaramillo Jonatán Obed , Anacleto Herrera Edgar Eduardo , Sevilla Villalobos Salvador 
Published: January 2019

El Área Metropolitana de Guadalajara ha pasado por una serie de procesos que la llevaron a convertirse en una metrópolis, donde el fenómeno de las urbanizaciones cerradas tomó relevancia significativa, lo cual implica un resultado directo de la falta de planeación urbana. Las facilidades que encontró el sector inmobiliario le permitieron posicionarse como principal actor en el proceso de construcción urbana, logrando que en el 2016 la ciudad contara con 2973 fraccionamientos de acceso restringido. Es un fenómeno que requiere la reinterpretación del espacio público de la ciudad y de sus espacios de uso colectivo.