bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skeletal muscle interstitial Po2 kinetics during recovery from contractions
Authors: Hirai Daniel M. , Craig Jesse C. , Colburn Trenton D. , Eshima Hiroaki , Kano Yutaka , Musch Timothy I. , Poole David C. 
Published: August 2019

The oxygen partial pressure in the interstitial space (Po2 is) drives O2 into the myocyte via diffusion, thus supporting oxidative phosphorylation. Although crucial for metabolic recovery and the capacity to perform repetitive tasks, the time course of skeletal muscle Po2 is during recovery from contractions remains unknown. We tested the hypothesis that Po2 is would recover to resting values and display considerable on-off asymmetry (fast on-, slow off-kinetics), reflective of asymmetric capillary hemodynamics. Microvascular Po2 (Po2 mv) was also evaluated to test the hypothesis that a significant transcapillary gradient (ΔPo2 = Po2 mv − Po2 is) would be sustained during recovery. Po2 mv and Po2 is (expressed in mmHg) were determined via phosphorescence quenching in the exposed rat spinotrapezius muscle during and after submaximal twitch contractions ( n = 12). Po2 is rose exponentially ( P < 0.05) from end-contraction (11.1 ± 5.1), such that the end-recovery value (17.9 ± 7.9) was not different from resting Po2 is (18.5 ± 8.1; P > 0.05). Po2 is off-kinetics were slower than on-kinetics (mean response time: 53.1 ± 38.3 versus 18.5 ± 7.3 s; P < 0.05). A significant transcapillary ΔPo2 observed at end-contraction (16.6 ± 7.4) was maintained throughout recovery (end-recovery: 18.8 ± 9.6; P > 0.05). Consistent with our hypotheses, muscle Po2 is recovered to resting values with slower off-kinetics compared with the on-transient in line with the on-off asymmetry for capillary hemodynamics. Maintenance of a substantial transcapillary ΔPo2 during recovery supports that the microvascular-interstitium interface provides considerable resistance to O2 transport. As dictated by Fick’s law (V̇o2 = Do2 × ΔPo2), modulation of O2 flux (V̇o2) during recovery must be achieved via corresponding changes in effective diffusing capacity (Do2; mainly capillary red blood cell hemodynamics and distribution) in the face of unaltered ΔPo2. NEW & NOTEWORTHY Capillary blood-myocyte O2 flux (V̇o2) is determined by effective diffusing capacity (Do2; mainly erythrocyte hemodynamics and distribution) and microvascular-interstitial Po2 gradients (ΔPo2 = Po2 mv − Po2 is). We show that Po2 is demonstrates on-off asymmetry consistent with Po2 mv and erythrocyte kinetics during metabolic transitions. A substantial transcapillary ΔPo2 was preserved during recovery from contractions, indicative of considerable resistance to O2 diffusion at the microvascular-interstitium interface. This reveals that effective Do2 declines in step with V̇o2 during recovery, as per Fick’s law.
BRN4 Is a Novel Driver of Neuroendocrine Differentiation in Castration-Resistant Prostate Cancer and Is Selectively Released in Extracellular Vesicles with BRN2
Authors: Bhagirath Divya , Yang Thao Ly , Tabatabai Z. Laura , Majid Shahana , Dahiya Rajvir , Tanaka Yuichiro , Saini Sharanjot 
Published: August 2019

Abstract

Purpose:
Neuroendocrine prostate cancer (NEPC), an aggressive variant of castration-resistant prostate cancer (CRPC), often emerges after androgen receptor–targeted therapies such as enzalutamide or de novo, via trans-differentiation process of neuroendocrine differentiation. The mechanistic basis of neuroendocrine differentiation is poorly understood, contributing to lack of effective predictive biomarkers and late disease recognition. The purpose of this study was to examine the role of novel proneural Pit-Oct-Unc-domain transcription factors (TF) in NEPC and examine their potential as noninvasive predictive biomarkers.
Experimental Design: Prostate cancer patient-derived xenograft models, clinical samples, and cellular neuroendocrine differentiation models were employed to determine the expression of TFs BRN1 and BRN4. BRN4 levels were modulated in prostate cancer cell lines followed by functional assays. Furthermore, extracellular vesicles (EV) were isolated from patient samples and cell culture models, characterized by nanoparticle tracking analyses, Western blotting, and real-time PCR.

Results:
We identify for the first time that: (i) BRN4 is amplified and overexpressed in NEPC clinical samples and that BRN4 overexpression drives neuroendocrine differentiation via its interplay with BRN2, a TF that was previously implicated in NEPC; (ii) BRN4 and BRN2 mRNA are actively released in prostate cancer EVs upon neuroendocrine differentiation induction; and (iii) enzalutamide treatment augments release of BRN4 and BRN2 in prostate cancer EVs, promoting neuroendocrine differentiation induction.

Conclusions:
Our study identifies a novel TF that drives NEPC and suggests that as adaptive mechanism to enzalutamide treatment, prostate cancer cells express and secrete BRN4 and BRN2 in EVs that drive oncogenic reprogramming of prostate cancer cells to NEPC. Importantly, EV-associated BRN4 and BRN2 are potential novel noninvasive biomarkers to predict neuroendocrine differentiation in CRPC.
Effect of Baseline Left Ventricular Ejection Fraction on 2-Year Outcomes After Transcatheter Aortic Valve Replacement
Authors: Furer Ariel , Chen Shmuel , Redfors Bjorn , Elmariah Sammy , Pibarot Philippe , Herrmann Howard C. , Hahn Rebecca T. , Kodali Susheel , Thourani Vinod H. , Douglas Pamela S. , Alu Maria C. , Fearon William F. , Passeri Jonathan , Malaisrie S. Chris , Crowley Aaron , McAndrew Thomas , Genereux Philippe , Ben-Yehuda Ori , Leon Martin B. , Burkhoff Daniel 
Published: August 2019

Background:
Impaired left ventricular function is associated with worse prognosis among patients with aortic stenosis treated medically or with surgical aortic valve replacement. It is unclear whether reduced left ventricular ejection fraction (LVEF) is an independent predictor of adverse outcomes after transcatheter aortic valve replacement.

Methods and Results:

Patients who underwent transcatheter aortic valve replacement in the PARTNER 2 trials (Placement of Aortic Transcatheter Valves) and registries were stratified according to presence of reduced LVEF (<50%) at baseline, and 2-year risk of cardiovascular mortality was compared using Kaplan–Meier methods and multivariable Cox proportional hazards regression. Of 2991 patients, 839 (28%) had reduced LVEF. These patients were younger, more often males, and were more likely to have comorbidities, such as coronary disease, diabetes mellitus, and renal insufficiency. Compared with patients with normal LVEF, patients with low LVEF had higher crude rates of 2-year cardiovascular mortality (19.8% versus 12.0%,
P
<0.0001) and all-cause mortality (27.4% versus 19.2%,
P
<0.0001). Mean aortic valve gradient was not associated with clinical outcomes other than heart failure hospitalizations (hazard ratio [HR], 0.99; CI, 0.99–1.00;
P
=0.03). After multivariable adjustment, patients with reduced versus normal LVEF had significantly higher adjusted risk of cardiovascular death (adjusted HR, 1.42, 95% CI, 1.11–1.81;
P
=0.005), but not all-cause death (adjusted HR, 1.20; 95% CI, 0.99–1.47;
P
=0.07). When LVEF was treated as continuous variable, it was associated with increased 2-year risk of both cardiovascular mortality (adjusted HR per 10% decrease in LVEF, 1.16; 95% CI, 1.07–1.27;
P
=0.0006) and all-cause mortality (adjusted HR, 1.09; 95% CI, 1.01–1.16;
P
=0.02).

Conclusions:
In this patient-level pooled analysis of PARTNER 2 patients who underwent transcatheter aortic valve replacement, baseline LVEF was an independent predictor of 2-year cardiovascular mortality.

Clinical Trial Registration:

URL:
https://www.clinicaltrials.gov
. Unique identifiers: NCT01314313, NCT02184442, NCT03222128, and NCT02184441.
Impact of preleukemic mutations and their persistence on hematologic recovery after induction chemotherapy for AML
Authors: Murphy Tracy , Zou Jinfeng , Daher-Reyes Georgina S. , Arruda Andrea , Gupta Vikas , McNamara Caroline J. , Minden Mark D. , Schimmer Aaron D. , Sibai Hassan , Yee Karen W. L. , Korulla Mariam , Stockley Tracy , Kamel-Reid Suzanne , Maze Dawn , Tierens Anne , Bratman Scott V. , Schuh Andre C. , Chan Steven M. 
Published: August 2019

Key Points
DNMT3A R882, TET2, ASXL1, and SRSF2 mutations identified at the time of diagnosis are associated with delayed count recovery. Persistence of preleukemic mutations in remission at high variant allele frequency is associated with delayed count recovery.
Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform
Authors: Ahn Su Jeong , Baek Yun Hee , Lloren Khristine Kaith S. , Choi Won-Suk , Jeong Ju Hwan , Antigua Khristine Joy C. , Kwon Hyeok-il , Park Su-Jin , Kim Eun-Ha , Kim Young-il , Si Young-Jae , Hong Seung Bok , Shin Kyeong Seob , Chun Sungkun , Choi Young Ki , Song Min-Suk 
Published: August 2019

Abstract
Background
In addition to seasonal influenza viruses recently circulating in humans, avian influenza viruses (AIVs) of H5N1, H5N6 and H7N9 subtypes have also emerged and demonstrated human infection abilities with high mortality rates. Although influenza viral infections are usually diagnosed using viral isolation and serological/molecular analyses, the cost, accessibility, and availability of these methods may limit their utility in various settings. The objective of this study was to develop and optimized a multiplex detection system for most influenza viruses currently infecting humans.

Methods
We developed and optimized a multiplex detection system for most influenza viruses currently infecting humans including two type B (both Victoria lineages and Yamagata lineages), H1N1, H3N2, H5N1, H5N6, and H7N9 using Reverse Transcriptional Loop-mediated Isothermal Amplification (RT-LAMP) technology coupled with a one-pot colorimetric visualization system to facilitate direct determination of results without additional steps. We also evaluated this multiplex RT-LAMP for clinical use using a total of 135 clinical and spiked samples (91 influenza viruses and 44 other human infectious viruses).

Results
We achieved rapid detection of seasonal influenza viruses (H1N1, H3N2, and Type B) and avian influenza viruses (H5N1, H5N6, H5N8 and H7N9) within an hour. The assay could detect influenza viruses with high sensitivity (i.e., from 100 to 0.1 viral genome copies), comparable to conventional RT-PCR-based approaches which would typically take several hours and require expensive equipment. This assay was capable of specifically detecting each influenza virus (Type B, H1N1, H3N2, H5N1, H5N6, H5N8 and H7N9) without cross-reactivity with other subtypes of AIVs or other human infectious viruses. Furthermore, 91 clinical and spiked samples confirmed by qRT-PCR were also detected by this multiplex RT-LAMP with 98.9% agreement. It was more sensitive than one-step RT-PCR approach (92.3%).

Conclusions
Results of this study suggest that our multiplex RT-LAMP assay may provide a rapid, sensitive, cost-effective, and reliable diagnostic method for identifying recent influenza viruses infecting humans, especially in locations without access to large platforms or sophisticated equipment.
Learning Health System for Breast Cancer: Pilot Project Experience
Authors: Levine Mark N. , Alexander Gordon , Sathiyapalan Arani , Agrawal Anjali , Pond Greg 
Published: August 2019

PURPOSE Clinicians need accurate and timely information on the impact of treatments on patient outcomes. The electronic health record (EHR) offers the potential for insight into real-world patient experiences and outcomes, but it is difficult to tap into. Our goal was to apply artificial intelligence technology to the EHR to characterize the clinical course of patients with stage III breast cancer. PATIENTS AND METHODS Data from patients with stage III breast cancer who presented between 2013 and 2015 were extracted from the EHR, de-identified, and imported into the IBM Cloud. Specialized natural language processing (NLP) annotators were developed to extract medical concepts from unstructured clinical text and transform them to structured attributes. In the validation phase, these annotators were applied to 19 additional patients with stage III breast cancer from the same period. The resulting data were compared with that in the medical chart (gold standard) for nine key indicators. RESULTS Information was extracted for 50 patients, including tumor stage (94% stage IIIA, 6% stage IIIB), age (28% 50 years or younger, 52% between 51 and 70 years, and 24% older than 70 years), receptor status (84% estrogen receptor positive, 74% progesterone receptor positive), and first treatment (72% surgery, 26% chemotherapy, 2% endocrine). Events in the patient’s journey were compiled to create a timeline. For 171 data elements, NLP and the chart disagreed for 41 (24%; 95% CI, 17.8% to 31.1%). With additional manipulation using simple logic, the disagreement was reduced to six elements (3.5%; 95% CI, 1.3% to 7.5%; F1 statistic, 0.9694). CONCLUSION It is possible to extract, read, and combine data from the EHR to view the patient journey. The agreement between NLP and the gold standard was high, which supports validity.
German New Lefts
Authors: Renaud Terence 
Published: August 2019

AbstractThe New Left that arose in West Germany during the 1960s mimicked the antifascist reformations of the 1930s. For grassroots campaigns, extraparliamentary opposition groups, and radical student organizations of the postwar decades, the Marxist humanist theories and revolutionary socialist splinter groups of the interwar years served as attractive models. At the same time, the Sixty-eighter generation rebelled against a political establishment now represented by that earlier generation of neoleftist pioneers, their parents. But generational conflict was just the symptom of a deeper problem in the history of the midcentury Left: a succession of radical new lefts arose out of periodic frustration at institutionalized politics. This article explores the missing link between Germany’s antifascist and antiauthoritarian new lefts: the so-called left socialists of the 1950s. In particular, Ossip K. Flechtheim’s science of futurology and Wolfgang Abendroth’s theory of antagonistic society translated antifascism’s legacies into a new paradigm of social protest. The left socialists’ support for the embattled Socialist German Student League laid the organizational and intellectual foundation for the sixties New Left. Recent studies of the “global sixties” have shown the transnational connections between new lefts across space; this article explains their continuity across time.
Memory and Repetition
Authors: Knittel Susanne 
Published: August 2019

AbstractIn recent years, reenactment has emerged as a compelling and controversial form of coming to terms with the past (Vergangenheitsbewältigung). As reconstructions of past events inflected through contemporary contexts and concerns, reenactments prompt an affective, empathetic, and ethical engagement with the past. This makes them a particularly provocative means of engaging with the figure of the perpetrator and with questions of guilt, responsibility, and agency. This article reads reenactments by Romuald Karmakar and Milo Rau as challenges to a teleological conception of history and simplistic binaries of good versus evil. Their works stage perpetrator documents in their entirety, without commentary. In this way they participate in the turn away from the “hermeneutics of suspicion” toward an affirmative critical practice. Without an interpretive “safety net,” the audience must engage with the reenacted event on its own terms, affirmatively, and thus risk the security of their preconceived positions. Only then can the repetition engender difference.
The Flight of the Gods
Authors: Rosenstock Bruce 
Published: August 2019

AbstractThe article argues that Martin Heidegger and Oskar Goldberg share a common gnostic motif of the “flight of the gods from the earth” and that both thinkers seek to prepare their people (the Germans, the Jews) for a “second beginning” of metaphysics that would restore the relationship between the people’s god and the earth. Both thinkers also reject the Darwinian concept of the “state as organism” in the new academic study of human geography known as Geopolitik. Both thinkers embrace a neovitalist concept of animal life as it was developed in the work of Jakob von Uexküll. Despite these commonalities, Heidegger and Goldberg differ in how they understand the place of human animality once this renewal takes place. Heidegger calls for the sacrificing of the people’s animality in their readiness to die for the “last god,” whereas Goldberg envisions the increasing power of the transcendental life force in the human species as a whole.
Paisagens sonoras, música e industria cultural: problematização na formação inicial de professores de física
Authors: de Oliveira Costa Junior José , Sutil Noemi , Pereira Alves João Amadeus 
Published: August 2019

Neste trabalho são explicitadas considerações sobre atividades educacionais envolvendo análise de paisagens sonoras, música e indústria cultural, desenvolvidas com estudantes de um curso de Licenciatura em Física, numa instituição de Ensino Superior na cidade de Curitiba, estado do Paraná, Brasil, em 2016. Este trabalho possui fundamentos na Teoria Crítica, conforme proposições de Theodor Adorno, na teoria da ação dialógica, de Paulo Freire, e em estudos de paisagens sonoras, de acordo com Raymond Murray Schafer. As ações compreendidas neste trabalho agregaram a identificação e a caracterização de elementos de paisagem sonora e música, com estabelecimento de relações com a indústria cultural. Nesse contexto foram constituídos dados por meio de observação direta, com registros escritos em diário de campo e gravações em áudio, assim como produções escritas dos participantes. Esses dados foram analisados conforme pressupostos e características de análise de conteúdo apresentada por Laurence Bardin. Entre os resultados podem ser destacados avanços em relação ao desenvolvimento de percepção auditiva, aprendizagem de conteúdos de Física, críticas à paisagem sonora observada, aspectos musicais e reflexão em relação às imposições da indústria cultural.
Effect of logistical control in customer satisfaction in a company in the packing sector
Authors: Gomes Alcir das Neves , Figueiredo Everton Santana 
Published: August 2019

Logistics has become a strategic activity along the time not only a mere operational task in corporations, for that reason having a suitable management for logistics processes bring benefits all over the companies and impacts customer satisfaction. This paper was developed based on a case study performed in a packaging company located in the east area of the city of São Paulo, it was conducted in the period from April to September of 2017. An evaluation of logistical processes was performed based on non-participative observations and posteriorly with participative observations, with the diagnosis of main problems corrective actions were implemented as well as processes control. A training program was done to prepare the workers to accomplish with the new procedures, that made possible to stablish a comparison between past results and the ones achieved with new procedures and controls. It was also proposed and utilized the Net Promoter Score – NPS method to evaluate customer satisfaction that allowed to observe the results evolution achieved with the new procedures implemented.
The flow deteriorations in course of the partial load operation of the middle specific speed Francis turbine
Authors: Szulc Przemyslaw , Machalski Artur 
Published: August 2019

Abstract
The article presents the results of experimental and numerical tests of a water turbine. As the object of the research the middle size Francis turbine, characterized by a middle value of specific speed, was taken into consideration. The primary aim of the realized project was to explain the occurrence of a high vibration level in a partial load operation. It was realized by the application of the superposition of experimental methods and a numerical simulation. The numerical results were verified on the basis of comparison with experimental results obtained for the real turbine. The numerical models were built with great accuracy to the reality and the flow was recognized, which allows to explain the reasons for random occurrence of high vibration in a draft tube and indicate a possibility of improvement of the analyzed object. The results of the numerical research such as distributions of velocity, pressure and vapor volume fraction in the passages were taken into consideration. The flow inside the Francis turbine and an outflow chamber were conscientiously analyzed. Pressure and velocity distribution as well as water surface structure were presented in the paper. Furthermore, the results numerical investigations helped to describe a cavitation rope vortex creation in the draft tube and the development of the air vortexes in the outlet chamber. Finally, the superposition of those phenomena was responsible for the deterioration of the turbine operation.
‚Cultural Turn‘ oder gar Paradigmenwechsel in der Althistorie? Die politische Kultur der römischen Republik in der neueren Forschung
Authors: Hölkeskamp Karl-Joachim 
Published: August 2019

Zusammenfassung
Die internationale althistorische Forschung zur Geschichte der römischen Republik hat sich in den letzten drei Jahrzehnten dynamisch weiterentwickelt. Dabei sind mehrere neue Schwerpunkte in den Vordergrund getreten: die fundamentale Bedeutung der direkten Interaktion und Kommunikation, insbesondere der politischen Rhetorik, in allen Prozessen der Beratung und Entscheidung einerseits und Praxis, Stil und Duktus der Rhetorik vor Volk, Gerichten und Senat andererseits; das komplexe Verhältnis der politisch-sozialen Elite zum Volk und die konkreten Formen, informellen und impliziten Regeln und Normen, Verfahren, Medien und Rituale des Umgangs mit dem Volk; die praktische, gewissermaßen technische Seite, die rituellen Dimensionen und die symbolischen Bedeutungen der Einbettung dieser Interaktion und Kommunikation in die dichte, vielfach vernetzte politisch-sakrale Topographie der urbs Roma als Stadtstaat; die Konzeptualisierung der politisch-sozialen Ordnung der Republik als einer spezifischen politischen Kultur, ihr Charakter und ihre Strukturen im besonderen Spannungsfeld zwischen Kontinuitäten und Prozessen des Wandels.
The crystal structure of tetrachlorido-bis{1,3-bis(2,6-diisopropylphenyl)-1H-3λ4-imidazol-2-yl}-(μ2-pyrimidine-κ2
N:N′)dipalladium(IV) — dichloromethane (1/2), C60H80Cl8N6Pd2
Authors: Zhao Xiao-Yun , Chen Lian-Hui 
Published: August 2019

Abstract
C60H80Cl8N6Pd2, triclinic, P1̄ (no. 2), a = 10.8703(10) Å, b = 17.0284(15) Å, c = 18.9595(17) Å, α = 83.1960(10)°, β = 88.909(2)°, γ = 71.523(2)°, V = 3304.5(5) Å3, Z = 2, R
gt(F) = 0.0488, wR
ref(F
2) = 0.1213, T = 130 K.