bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vacuolar pH regulates clathrin-mediated endocytosis through TORC1 signaling during yeast replicative aging
Authors: Antenor Kenneth Gabriel , Lee-Dadswell Jaime , Grishchenko Nina , Swaleh Shaimaa , Spangaro Allie , Usaj Mojca Mattiazzi 
Published: November 2024

ABSTRACTClathrin-mediated endocytosis (CME) is a critical cellular process that regulates nutrient uptake, membrane composition and signalling. While cellular aging is associated with functional changes across many cellular components contributing to the collective decline in cellular function, little is known about how it affects CME. Here we show that CME dynamics are significantly altered during replicative aging in budding yeast, with older cells having slower assembly of early and coat CME modules, resulting in longer endocytic turnover and reduced cargo internalization. This change in CME dynamics is mother cell-specific and is not observed in daughter cells. We identified vacuolar pH, a key driver of aging phenotypes in budding yeast, as a central player in this modulation of CME dynamics during aging. Perturbing vacuolar pH in young cells mimics aging-like CME dynamics, while maintaining an acidic vacuolar pH in aging cells preserves CME dynamics typical of young cells. Finally, we demonstrate that the vacuolar pH effect on CME is regulated through TORC1 via the effector kinase Npr1. These findings establish vacuolar pH as a critical regulator of CME during cellular aging, and strengthen its role in the overall cellular aging process in budding yeast.
Revealing the diversity of commensal corynebacteria from a single human skin site
Authors: Herman Reyme , Meaden Sean , Rudden Michelle , Cornmell Robert , Wilkinson Holly N. , Hardman Matthew J. , Wilkinson Anthony J. , Murphy Barry , Thomas Gavin H. 
Published: November 2024

AbstractOur understanding of the skin microbiome has dramatically improved since the pioneering studies and the improvements in sequencing technologies. Species of the genusCorynebacteriumare known to form a major part of the human skin microbiome but most detailed studies have focussed on other similarly prevalent genera likeStaphylococcusandCutibacteria. Prior to this study, there were few complete genomes for skin commensals of the genusCorynebacterium, with only 9 complete genomes available for the most commonly identified speciesC. tuberculostearicum. In this study we explored the genusCorynebacteriumfrom a single body site by swabbing the axilla/underarm of 4 individuals and using a selective media to enrich for corynebacteria. We then generated whole genome sequencing data of these corynebacteria enriched isolates using long-read sequencing and subsequent bioinformatics analysis to reveal an unparalleled diversity of this genus from a single skin site. Through this approach, we obtained the closed genomes of 215 isolates, 154 derived from a single individual. With this genetic information, we were able to identify 7 different species including species previously not associated to the skin and two novel species provisionally namedC. axilliensisandC. jamesii. We used pangenome analysis on 30 genetically distinct isolates spanning the 7 species to identify putative metabolic differences, antimicrobial resistance profiles, novel biosynthetic gene clusters (BGCs), prophages and phage defence systems. Our culture-based Nanopore sequencing approach has dramatically improved our overall knowledge of skin corynebacteria, and uniquely here also providing in-depth analysis from a single skin site, revealing a multitude of differences between the isolates. Here we not only improved our knowledge of axillary corynebacteria but also greatly expanded the publicly available number of cutaneous corynebacterial genomes complementing recent studies seeking to understand the diversity of skin corynebacteria.
Bidirectionally responsive thermoreceptors encode cool and warm
Authors: Bokiniec Phillip , Whitmire Clarissa J. , Poulet James F.A. 
Published: November 2024

AbstractThermal sensation is a fundamental sense initiated by the activity of primary afferent thermoreceptors. While considerable attention has been paid to the encoding of noxious temperatures by thermoreceptors, it is far less clear how they encode innocuous cool and warm which are more commonly encountered in the environment. To address this, we sampled the entire thermoreceptor population usingin vivotwo-photon calcium imaging in the lumbar dorsal root ganglia of awake and anesthetized mice. We found that the vast majority of thermoreceptors respond bidirectionally, with an enhanced response to cool and a suppressed response to warm. Usingin vivopharmacology and computational modelling, we demonstrate that conductance changes in the cool-sensitive TRPM8 channel are sufficient to explain this bidirectional response type. Our comprehensive dataset reveals the fundamental principles of the peripheral encoding of innocuous temperatures and suggests that the same population of thermoreceptors underlie the distinct sensations of cool and warm.
Characterization of widely conserved novel pigment production inBacillus subtilisspecies
Authors: Stannius Rune Overlund , Dunlap Christopher A. , Morvan Estelle , Berbon Mélanie , Lecomte Sophie , Loquet Antoine , Kovács Ákos T. 
Published: November 2024

AbstractBacillus subtilisis widely studied in the microbial secondary metabolite (SM) field due to its rich variety of important natural products and genetic tractability. However, identification of novel SMs and their biosynthetic gene cluster (BGCs) has become increasingly difficult, especially inBacilli, as the tools for screening and genome mining are dependent on clear function or similarity to already known BGCs. Pigments are SMs identified by their absorption of visible light, resulting in a certain color perceived by our eyes at sufficient concentrations. Thereby, pigments provide the evidence of a BGC without knowing the sequence or function. Expanding the known repertoire of SM BGCs with novel BGCs will further reinforce identification of a broader set of BGCs by mining tools such as antiSMASH. Here, we study a pigment observed inB. subtilissoil isolate MB9_B4 on certain media. We characterize the conditions where this pigment is produced and identify the corresponding BGC using a comparative genomic approach exploiting our strain collection containing other isolates with pigment production ability. The responsible BGC carried several genes, which were annotated as parts of the tryptophan biosynthesis pathway, possibly originating from a duplication and divergence of an originally primary metabolism. Identification of the pigment gene cluster additionally lead to the discovery of additional pigment BGC carrierB. subtilisisolates, some of which were described at the earliest in 1896 under the nameBacillus aterrimus, with a name referring to a dark pigmentation (the Latin “aterrimus” meaning very black). In addition, we employed solid-state nuclear magnetic resonance and Fourier transform infrared spectroscopies to characterize the chemical groups of the pigment. This study describes the chemical and biological features of a new class of SM BGC, which we hope will serve to improve the current BGC discovery pipelines inBacilli.
Using deep learning to predict internalizing problems from brain structure
Authors: Vandewouw Marlee M. , Syed Bilal , Barnett Noah , Arias Alfredo , Kelley Elizabeth , Jones Jessica , Ayub Muhammad , Iaboni Alana , Arnold Paul D. , Crosbie Jennifer , Schachar Russell J , Taylor Margot J , Lerch Jason P. , Anagnostou Evdokia , Kushki Azadeh 
Published: November 2024

AbstractInternalizing problems are associated with a wide range of adverse outcomes. While we have some understanding about risk factors (e.g., neurodevelopmental conditions), biological markers are not well understood. Here, we used deep learning to predict cross-sectional (N=14,523) and worsening longitudinal trajectories (N=10,540) of internalizing problems from measures of brain structure. A stratified cross-validation scheme was used, and performance was evaluated using the area under the receiving operating characteristic curve (AUC). The cross-sectional model performed well across the sample, reaching an AUC of 0.80 [95% CI: 0.71, 0.88]. For the longitudinal model, while performance was sub-optimal for predicting worsening trajectories in a sample of the general population (AUC=0.66 [0.65, 0.67]), good performance was reached across individuals with a neurodevelopmental condition (AUC=0.73 [0.70, 0.76]). Deep learning with features of brain structure is a promising avenue for biomarkers of internalizing problems, particularly for individuals who have a higher likelihood of experiencing difficulties.
Higher frequency of transition mutation over transversion mutation in genomes: evidence from the binding energy calculation of base pairs using DFT
Authors: Deka Nishita , Gour Nand Kishor , Pant Pradeep , Satapathy Siddhartha Shankar , Hoda Nasimul , Deka Ramesh Chandra , Ray Suvendra Kumar 
Published: November 2024

AbstractBase substitution mutations such as transition (ti) and transversion (tv) in organisms are major driving force in molecular evolution. In this study, different possible types of base pairing that can causetiandtvwere investigated using the density functional theory (DFT) method. The chemical structures of bases as well as base pairs were optimized using B3LYP hybrid functional along with 6-31G(d,p) basis set. We performed single point energy calculation of all optimized species using the same functional but combined with higher diffuse and polarized basis set i.e. 6-311++G(d,p) to get more refined energy of all species. The binding energy of various base pairs was calculated considering basis set superposition error (BSSE) as well as without BSSE. The binding energy of the base pairs leading totiwas found to be more stable than that of the base pairs leading totv. This was interesting considering the observations in organisms thattisare more frequent thantvs. Among the base pairs leading to the sameti, G(keto): T (enol)base pair was found to be more stable than A(imino):C(amino)base pair. This theoretical study of binding energy of different base pairs using the DFT method has provided additional evidences in support to the biological observations of a higher transition rate than transversion in genomes.
GABRA1frameshift variants impair GABAAreceptor proteostasis
Authors: Williams Marnie P. , Wang Ya-Juan , Kang Jing-Qiong , Perryman John H. , Mu Ting-Wei 
Published: November 2024

AbstractThe gamma-aminobutyric acid type A receptor (GABAAR) is the most common inhibitory neurotransmitter-gated ion channel in the central nervous system. Pathogenic variants in genes encoding GABAAR subunits can cause receptor dysfunction and lead to genetic epilepsy. Frameshift variants in these genes can result in a premature termination codon, producing truncated receptor subunit variants. However, the pathogenic molecular mechanism as well as functional implications of these frameshift variants remains inadequately characterized. This study focused on four clinical frameshift variants of the α1subunit of GABAAR (encoded by theGABRA1gene): K401fs (c.1200del), S326fs (c.975del), V290fs (c.869_888del), and F272fs (c.813del). These variants result in the loss of one to three transmembrane helices, whereas wild type α1 has four transmembrane helices. Therefore, these variants serve as valuable models to evaluate membrane protein biogenesis and proteostasis deficiencies of GABAARs. In HEK293T cells, all four frameshift variants exhibit significantly reduced trafficking to the cell surface, resulting in essentially non-functional ion channels. However, the severity of proteostasis deficiency varied among these four frameshift variants, presumably due to their specific transmembrane domain deletions. The variant α1subunits exhibited endoplasmic reticulum (ER) retention and activated the unfolded protein response (UPR) to varying extents. Our findings revealed that these frameshift variants ofGABRA1utilize overlapping yet distinct molecular mechanisms to impair proteostasis, providing insights into the pathogenesis of GABAAR-associated epilepsy.
Challenges and strategies related to serving customers with food allergies in private clubs in the US
Authors: Wen Han 
Published: November 2024

PurposeThe purpose of this study was to explore the current issues and challenges related to serving customers with food allergies in club foodservice operations.Design/methodology/approachA total of 15 individual interviews were conducted with club managers in the US. Participants shared their strategies for preventing food allergy incidents, such as taking advantage of the member information management system, ensuring a proper communication line and preventing cross-contacts in both the front-of-the-house (FOH) and back-of-the-house (BOH).FindingsResults indicated that food allergies posed challenges for club managers regarding the increasing populations and types of food allergies, customers not communicating their food allergies and the issues relating to providing allergen-free foods in large banquets. Balancing safety and satisfaction is critically important due to the nature of club operations.Research limitations/implicationsImplications to both club foodservice operators and customers with food allergies were provided.Originality/valueThis is the first research focusing on challenges and issues related to serving customers with food allergies in the club industry.
Unveiling knowledge dynamics for competitive advantage: insights into hiding, sharing and big data management
Authors: Rehman Shafique Ur , Bresciani Stefano , Riaz Adil , Giovando Guido 
Published: November 2024

PurposeThis study investigates the influence of knowledge hiding in organizations (KHOs), knowledge sharing in organizations (KSOs) and big data knowledge management (BDKM) on knowledge management success. Monitoring (MNT) is used as a moderator between KHO and knowledge management success. Besides, knowledge management success and digital business intensity (DBI) are used to attain competitive advantage (CA). Finally, digital business intensity is used as a moderator between knowledge management success and competitive advantage.Design/methodology/approachData were collected through questionnaires using simple random sampling, and a total of 433 questionnaires were used in the final analysis. Partial least squares structural equation modeling is used to test study hypotheses.FindingsResults indicate that KSO, BDKM and monitoring are positive, but KHO is negatively related to knowledge management success. Monitoring moderates the relationship between KHO and knowledge management success. Knowledge management success and digital business intensity significantly attain competitive advantage. Digital business intensity significantly moderates the relationship between knowledge management success and competitive advantage.Practical implicationsTop managers can use study findings in decision-making if they want to attain a competitive advantage. KHO has a negative influence on knowledge management success, but this relationship can be changed by monitoring the employees. KSO, BDKM, knowledge management success and digital business intensity play a vital role in determining competitive advantage.Originality/valueBy building knowledge culture using the knowledge-based view, this study contributes to KHO, KSO, BDKM, monitoring, digital business intensity and competitive advantage literature.
Quality of working life in remote platform work: a systematic review of a fragmented field of research
Authors: Marà Claudia , Vermeerbergen Lander , Pulignano Valeria , Hannes Karin 
Published: November 2024

PurposeRevitalisation of quality of working life (QWL) research points to non-standard work such as remote platform work as a compelling setting where research on QWL is needed. Whereas the literature on working conditions in remote work platforms is rich, knowledge on the topic is fragmented. This systematic review aims to synthesize and integrate findings from existing literature to offer an encompassing and multidimensional understanding of QWL and the managerial practices linked to it in remote work platforms.Design/methodology/approachA systematic review of 24 empirical qualitative studies selected based on a multiple-database search using Boolean search engines. The selection of studies to be included in the review was performed through a four-steps procedure, following the PRISMA protocol. A thematic analysis of the studies was performed to synthesize findings.FindingsWe synthesize and show how remote platform workers experience a degrading QWL along five QWL dimensions, and we illustrate how these QWL dimensions are influenced by platforms’ managerial practices such as client-biased systems, rate-setting mechanisms, reputational systems, global competition schemes, lock-in systems, monitoring and nudging systems and information asymmetry.Originality/valueThe study contributes to reinvigorating QWL literature by producing a systematic synthesis of workers’ experience of QWL in the non-standard work context of remote platform work and the managerial practices that influence QWL. Our study overcomes two main shortcomings of the existing empirical studies published: (1) studies examine only a few QWL dimensions and/or (2) examine some platforms’ managerial practices that influence QWL, overlooking others.
Effect of squeeze motion on the tribo-performances of molybdenum disulfide powder lubricated textured cemented carbide specimens
Authors: Pang Minghua , Li Zhenjiang , Hu Yikun , Gan Zichen , Ma Lijie , Feng QigaoFeng 
Published: November 2024

Purpose
This study aims to improve the lubrication performance of molybdenum disulfide powders at textured surface of cemented carbide materials, a squeeze motion of vibration assistance method was introduced and investigated.

Design/methodology/approach
Surface texture was fabricated on YT15 cemented carbide samples using a laser marking machine. After that, a tribological experiment was carried out on a self-built friction testing machine under different amplitude and frequency of squeeze motion conditions. Moreover, a simulation model was also established to verify the principle of squeeze motion on the lubrication performance improving of MoS2 particles at textured interfaces.

Findings
Analysis results indicated that surface texture on test sample can increase the storage ability of solid lubrication particles, and the lubrication film at the contact interface is more easily formed due to the reciprocating action. Squeeze motion can improve the storage ability of it due to an intermittent contact, which provides an opportunity for MoS2 particles infiltration, and then a more uniform distribution and load-bearing properties of force chain are also established and formed simultaneously. Thus, a better tribological performance at the contact interface is obtained.

Originality/value
The main contribution of this work is to provide a reference for the molybdenum disulfide powder lubrication with textured surface of cemented carbide materials.

Peer review
The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-05-2024-0166/
The relationship between eWoM, security, quality of information and purchase behaviour at B2C e-commerce website: the moderating role of E-trustmarks
Authors: Quan Nguyen Hong , Ha Pham Thi , Tuan Vi Thanh , Nam Nguyen Hoai , Trang Le Thi Thuy , Mai Nguyen Truc 
Published: November 2024

PurposeThis study aims to investigate the moderating role of e-trustmarks in the relationship between electronic word of mouth (eWoM), security, quality of information, and purchase behaviour of consumers on B2C e-commerce websites. The research was conducted in the context of emerging countries.Design/methodology/approachThe research utilised partial least squares path modelling (PLS-SEM) to assess the model and its hypothesised relationships, drawing on data from a survey of 1993 consumers engaged in e-commerce websites with e-trustmarks. The study incorporated SOR theory, asymmetric information theory, and signalling theory.FindingsE-trustmarks moderate the positive impact of eWoM, security, and quality of information on online trust. A moderating effect of e-trustmarks was also found, such that the positive association between purchase intention and purchase behaviour was stronger when e-trustmarks were higher.Research limitations/implicationsThis study was conducted solely in Vietnam and focused only on a set of input variables without addressing specific industries or market segments. The research did not use demographic data, such as the age and income of the surveyors, to identify variations in the impact of variables on different target groups, indicating potential areas for future research.Practical implicationsEmerging countries should implement targeted policies regarding e-trustmarks and encourage businesses to adopt them to reduce information asymmetry in the online environment.Originality/valueThis study contributes to asymmetric theory and signalling theory by offering critical insights into how online businesses can make use of influence mechanisms in the model to ease asymmetric information. Integrating signalling theory and moderating e-trustmarks into the SOR model advances research on consumer behaviour in emerging countries’ e-commerce markets with asymmetric information.
Examining the impact of translanguaging-enriched argument mapping on higher education EFL students’ argumentative writing skills
Authors: Robillos Roderick Julian 
Published: November 2024

PurposeThis research explores the effects of combining argument mapping (AM) with translanguaging pedagogy on the argumentative writing abilities of third-year university English as a Foreign Language students (EFL) at a Thai university. The aim is to explore synergies between AM and translanguaging to enhance students’ argumentative writing skills and contribute to language education literature.Design/methodology/approachA sequential mixed-method research type, utilizing a single-group pre- and post-test design, was employed. About 27 third-year EFL university students participated in 13 sessions of AM with translanguaging to facilitate their argumentative writing tasks.FindingsQuantitative results revealed a significant difference across development of writing content and development of writing coherence as manifested in their pre- and post-test scores. ANOVA showed significant differences in students’ quiz writing performances. Additionally, the participants perceived translanguaging as a normative and respectful practice, despite being EFL learners. Qualitative findings indicated participants embraced the use of AM with translanguaging, acknowledging its benefits in enhancing their writing tasks.Originality/valueThis study highlights the innovative approach of this integration, not only for improving argumentative writing skills but also for fostering cognitive and linguistic fluency, along with critical thinking skills. This contributes to language education literature, urging further exploration of AM with translanguaging in diverse educational contexts.