bluuebunny/crossref_metadata_2025_split huggingface dataset:
The unique functioning of a pre-Columbian Amazonian floodplain fishery
Authors: Blatrix Rumsaïs , Roux Bruno , Béarez Philippe , Prestes-Carneiro Gabriela , Amaya Marcelo , Aramayo Jose Luis , Rodrigues Leonor , Lombardo Umberto , Iriarte Jose , de Souza Jonas Gregorio , Robinson Mark , Bernard Cyril , Pouilly Marc , Durécu Mélisse , Huchzermeyer Carl F. , Kalebe Mashuta , Ovando Alex , McKey Doyle 
Published: April 2018

AbstractArchaeology provides few examples of large-scale fisheries at the frontier between catching and farming of fish. We analysed the spatial organization of earthen embankments to infer the functioning of a landscape-level pre-Columbian Amazonian fishery that was based on capture of out-migrating fish after reproduction in seasonal floodplains. Long earthen weirs cross floodplains. We showed that weirs bear successive V-shaped features (termed ‘Vs’ for the sake of brevity) pointing downstream for outflowing water and that ponds are associated with Vs, the V often forming the pond’s downstream wall. How Vs channelled fish into ponds cannot be explained simply by hydraulics, because Vs surprisingly lack fishways, where, in other weirs, traps capture fish borne by current flowing through these gaps. We suggest that when water was still high enough to flow over the weir, out-migrating bottom-hugging fish followed current downstream into Vs. Finding deeper, slower-moving water, they remained. Receding water further concentrated fish in ponds. The pond served as the trap, and this function shaped pond design. Weir-fishing and pond-fishing are both practiced in African floodplains today. In combining the two, this pre-Columbian system appears unique in the world.
Elimination of TDP-43 inclusions linked to amyotrophic lateral sclerosis by a misfolding-specific intrabody with dual proteolytic signals
Authors: Tamaki Yoshitaka , Shodai Akemi , Morimura Toshifumi , Hikiami Ryota , Minamiyama Sumio , Ayaki Takashi , Tooyama Ikuo , Furukawa Yoshiaki , Takahashi Ryosuke , Urushitani Makoto 
Published: April 2018

AbstractAggregation of TAR DNA-binding protein of 43 kDa (TDP-43) is implicated in the pathogenesis of sporadic and certain familial forms of amyotrophic lateral sclerosis (ALS), suggesting elimination of TDP-43 aggregates as a possible therapeutic strategy. Here we generated and investigated a single-chain variable fragment (scFv) derived from the 3B12A monoclonal antibody (MAb) that recognises D247 of the TDP-43 nuclear export signal, an epitope masked in the physiological state. In transfected HEK293A cells, 3B12A scFv recapitulated the affinity of the full-length MAb to mislocalised TDP-43 with a defective nuclear localising signal and to a TDP-43 inclusion mimic with cysteine-to-serine substitution at RRM1. Moreover, 3B12A scFv accelerated proteasome-mediated degradation of aggregated TDP-43, likely due to an endogenous PEST-like proteolytic signal sequence in the VH domain CDR2 region. Addition of the chaperone-mediated autophagy (CMA)-related signal to 3B12A scFv induced HSP70 transcription, further enhancing TDP-43 aggregate clearance and cell viability. The 3B12A scFv also reduced TDP-43 aggregates in embryonic mouse brain followingin uteroelectroporation while causing no overt postnatal brain pathology or developmental anomalies. These results suggest that a misfolding-specific intrabody prone to synergistic proteolysis by proteasomal and autophagic pathways is a promising strategy for mitigation of TDP-43 proteinopathy in ALS.
Cystoid edema, neovascularization and inflammatory processes in the murine Norrin-deficient retina
Authors: Beck Susanne C. , Karlstetter Marcus , Garcia Garrido Marina , Feng Yuxi , Dannhausen Katharina , Mühlfriedel Regine , Sothilingam Vithiyanjali , Seebauer Britta , Berger Wolfgang , Hammes Hans-Peter , Seeliger Mathias W. , Langmann Thomas 
Published: April 2018

AbstractMutations in the Norrin (NDP) gene cause severe developmental blood vessel defects in the retina leading to congenital blindness. In the retina of Ndph-knockout mice only the superficial capillary network develops. Here, a detailed characterization of this mouse model at late stages of the disease usingin vivoretinal imaging revealed cystoid structures that closely resemble the ovoid cysts in the inner nuclear layer of the human retina with cystoid macular edema (CME). In human CME an involvement of Müller glia cells is hypothesized. In Ndph-knockout retinae we could demonstrate that activated Müller cells were located around and within these cystoid spaces. In addition, we observed extensive activation of retinal microglia and development of neovascularization. Furthermore,ex vivoanalyses detected extravasation of monocytic cells suggesting a breakdown of the blood retina barrier. Thus, we could demonstrate that also in the developmental retinal vascular pathology present in the Ndph-knockout mouse inflammatory processes are active and may contribute to further retinal degeneration. This observation delivers a new perspective for curative treatments of retinal vasculopathies. Modulation of inflammatory responses might reduce the symptoms and improve visual acuity in these diseases.
Highly extended filaments in aqueous gold nano-particle colloidals
Authors: Yuan Shuai , Liu Feng J. , Wang Li R. , Nan Jun Y. , Li Min , He Bo Q. , Zeng He P. 
Published: April 2018

AbstractA new regime of filamentation has been discovered in aqueous gold nanoparticle colloidals (AGNC). Different from filamentation in liquids, in this regime, by doping water with gold nanoparticles, there is no observable multiple small-scale filaments, but instead a spatially continuous plasma channel is formed. The length of the filament is more than ten times as compared with that in water. Filamentation in AGNC is characterized by a colorful light channel, with generated supercontinuum ranging from 400 nm to 650 nm which is scattered along a cyan-orange path.
Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630
Authors: DeLorenzo Drew M. , Moon Tae Seok 
Published: April 2018

AbstractRhodococcus opacus PD630 is a gram-positive bacterium with promising attributes for the conversion of lignin into valuable fuels and chemicals. To develop an organism as a cellular factory, it is necessary to have a deep understanding of its metabolism and any heterologous pathways being expressed. For the purpose of quantifying gene transcription, reverse transcription quantitative PCR (RT-qPCR) is the gold standard due to its sensitivity and reproducibility. However, RT-qPCR requires the use of reference genes whose expression is stable across distinct growth or treatment conditions to normalize the results. Unfortunately, no in-depth analysis of stable reference genes has been conducted in Rhodococcus, inhibiting the utilization of RT-qPCR in R. opacus. In this work, ten candidate reference genes, chosen based on previously collected RNA sequencing data or literature, were examined under four distinct growth conditions using three mathematical programs (BestKeeper, Normfinder, and geNorm). Based on this analysis, the minimum number of reference genes required was found to be two, and two separate pairs of references genes were identified as optimal normalization factors for when ribosomal RNA is either present or depleted. This work represents the first validation of reference genes for Rhodococcus, providing a valuable starting point for future research.
Unique coexistence of dispersion stability and nanoparticle chemisorption in alkylamine/alkylacid encapsulated silver nanocolloids
Authors: Aoshima Keisuke , Hirakawa Yuya , Togashi Takanari , Kurihara Masato , Arai Shunto , Hasegawa Tatsuo 
Published: April 2018

AbstractSurface encapsulation of metal nanoparticles (NPs) is fundamental to achieve sufficient dispersion stability of metal nanocolloids, or metal nanoink. However, the feature is incompatible with surface reactive nature of the metal NPs, although these features are both essential to realizing the functional applications into printed electronics technologies. Here we show that two different kinds of encapsulation for silver NPs (AgNPs) by alkylamine and alkylacid together are the key to achieve unique compatibility between the high dispersion stability as dense nanoclolloids and the AgNP chemisorption printing on activated patterned polymer surfaces. Advanced confocal dynamic light scattering study reveals that an additive trace amount of oleic acid is the critical parameter for controlling the dispersion and coagulative (or surface-reactive) characteristics of the silver nanocolloids. The composition of the disperse media is also important for obtaining highly concentrated but low-viscosity silver nanocolloids that show very stable dispersion. The results demonstrate that the high-resolution AgNP chemisorption printing is possible only by using unique silver nanocolloids composed of an exceptional balance of ligand formulation and dispersant composition.
Photoluminescence quenching of dye molecules near a resonant silicon nanoparticle
Authors: Zyuzin Mikhail V. , Baranov Denis G. , Escudero Alberto , Chakraborty Indranath , Tsypkin Anton , Ushakova Elena V. , Kraus Florain , Parak Wolfgang J. , Makarov Sergey V. 
Published: April 2018

AbstractLuminescent molecules attached to resonant colloidal particles are an important tool to study light-matter interaction. A traditional approach to enhance the photoluminescence intensity of the luminescent molecules in such conjugates is to incorporate spacer-coated plasmonic nanoantennas, where the spacer prevents intense non-radiative decay of the luminescent molecules. Here, we explore the capabilities of an alternative platform for photoluminescence enhancement, which is based on low-loss Mie-resonant colloidal silicon particles. We demonstrate that resonant silicon particles of spherical shape are more efficient for photoluminescence enhancement than their plasmonic counterparts in spacer-free configuration. Our theoretical calculations show that significant enhancement originates from larger quantum yields supported by silicon particles and their resonant features. Our results prove the potential of high-index dielectric particles for spacer-free enhancement of photoluminescence, which potentially could be a future platform for bioimaging and nanolasers.
Emergence of Human G2P[4] Rotaviruses in the Post-vaccination Era in South Korea: Footprints of Multiple Interspecies Re-assortment Events
Authors: Thanh Hien Dang , Tran Van Trung , Lim Inseok , Kim Wonyong 
Published: April 2018

AbstractAfter the introduction of two global rotavirus vaccines, RotaTeq in 2007 and Rotarix in 2008 in South Korea, G1[P8] rotavirus was the major rotavirus genotype in the country until 2012. However, in this study, an emergence of G2P[4] as the dominant genotype during the 2013 to 2015 season has been reported. Genetic analysis revealed that these viruses had typical DS-1-like genotype constellation and showed evidence of re-assortment in one or more genome segments, including the incorporation of NSP4 genes from strains B-47/2008 from a cow and R4/Haryana/2007 from a buffalo in India, and the VP1 and VP3 genes from strain GO34/1999 from a goat in Bangladesh. Compared to the G2 RotaTeq vaccine strain, 17–24 amino acid changes, specifically A87T, D96N, S213D, and S242N substitutions in G2 epitopes, were observed. These results suggest that multiple interspecies re-assortment events might have contributed to the emergence of G2P[4] rotaviruses in the post-vaccination era in South Korea.
Modeling the Nature of Grammar and Vocabulary Trajectories From Prekindergarten to Third Grade
Authors:   Language and Reading Research Consortium (LARRC) Laura Justice Kate Cain Hui Jiang Jessica Logan Rongfang Jia, Jiang Hui , Logan Jessica A. , Jia Rongfang 
Published: April 2018

Purpose
This study investigated the longitudinal development of 2 important contributors to reading comprehension, grammar, and vocabulary skills. The primary interest was to examine the trajectories of the 2 skill areas from preschool to 3rd grade.

Method
The study involved a longitudinal sample of 420 children from 4 sites. Language skills, including grammar and vocabulary, were assessed annually with multiple measures. Multivariate latent growth curve modeling was used to examine the developmental trajectories of grammar and vocabulary, to test the correlation between the 2 domains, and to investigate the effects of demographic predictors on language growth.

Results
Results showed that both grammar and vocabulary exhibited decelerating growth from preschool to Grade 2. In Grade 3, grammar growth further flattened, whereas vocabulary continued to grow stably. Growth of vocabulary and grammar were positively correlated. Demographic characteristics, such as child gender and family socioeconomic status, were found to predict the intercept but not the slope of the growth trajectories.

Conclusions
Children's growth in grammar skills is differentiated in a number of important ways from their growth in vocabulary skills. Results of this study suggest the need to differentiate these dimensions of language when seeking to closely examine growth from preschool to primary grades.
3D printed microwave cavity for atomic clock applications: proof of concept
Authors: Pellaton M. , Affolderbach C. , Skrivervik A.K. , Ivanov A.E. , Debogovic T. , de Rijk E. , Mileti G. 
Published: April 2018

The authors present the realisation and characterisation of an additively manufactured (AM) microwave resonator cavity for double‐resonance (DR) vapour‐cell atomic clocks. The design of the compact microwave cavity is based on the loop‐gap resonator approach, previously demonstrated for conventionally‐machined aluminium components. In the present study, the resonator is fabricated by AM using a metal‐coated polymer. A resonance frequency at the desired 6.835 GHz rubidium atomic frequency is obtained. When employed in an atomic clock setup, the AM cavity enables a DR signal of <500 Hz linewidth and of nearly 20% contrast, thus fulfilling the stringent requirements for DR atomic clocks. A clock short‐term stability of 1 × 10−12 τ−1/2 is demonstrated, comparable to state‐of‐the‐art clock performances.
A complete sample of LSP blazars fully described in γ-rays
Authors: Arsioli B. , Polenta G. 
Published: April 2018

Context. We study the γ-ray and broadband spectral energy distribution (SED) properties of a complete sample of 104 bright, radio-selected low-synchrotron peaked (LSP) blazars, which have well-characterized SEDs from radio to X-rays. Most of the sources have already been detected in the γ-ray band by Fermi-LAT, however almost 20% of these blazars have no counterpart in any of the Fermi catalogs published so far.
Aims. Using the Fermi Science Tools, we look for γ-ray emission for those objects not yet reported in any Fermi-LAT catalog, finding new detections and associations. We then study the multifrequency SED for all sources in our sample, fitting their synchrotron (Syn) and inverse Compton (IC) components. A complete sample of LSP blazars with a full description in γ-ray is unique. We use this sample to derive the distribution of the Compton dominance (CD) along with population properties such as Syn and IC peak power, and frequency distributions.
Methods. We performed a binned likelihood analysis in the 0.3–500 GeV energy band with Fermi-LAT Pass 8 data, integrating over 7.5 yr of observations. We studied γ-ray light curves and test statistic (TS) maps to validate new detections and associations, thereby building a better picture of the high-energy activity in radio-selected LSP blazars. We fit the IC component for the new detections using all data at our disposal from X-rays to GeV γ-rays, enhancing the amount of information available to study the Syn to IC peak-power correlations.
Results. We deliver a unique characterization in γ-rays for a complete sample of LSP blazars. We show that three previously unidentified 3FGL sources can be associated with blazars when using improved γ-ray positions obtained from TS maps. Six previously unreported γ-ray sources are detected at TS > 20 level, while another three show TS values between 10–20. We evaluate two cases in which source confusion is likely present. In four cases there is no significant γ-ray signature when integrating over 7.5 yr. Short-lived flares at ~1 month scale, however, have been detected in these sources. Finally, we measure the log(CD) for the sample, which has a Gaussian-like distribution with median log(CD) ≈ 0.1, implying that on average the peak-power for the Syn and IC components in LSP blazars is similar.
Main sequence of star forming galaxies beyond the Herschel confusion limit
Authors: Pearson W. J. , Wang L. , Hurley P. D. , Małek K. , Buat V. , Burgarella D. , Farrah D. , Oliver S. J. , Smith D. J. B. , van der Tak F. F. S. 
Published: April 2018

Context. Deep far-infrared (FIR) cosmological surveys are known to be affected by source confusion, causing issues when examining the main sequence (MS) of star forming galaxies. In the past this has typically been partially tackled by the use of stacking. However, stacking only provides the average properties of the objects in the stack.
Aims. This work aims to trace the MS over 0.2 ≤ z < 6.0 using the latest de-blended Herschel photometry, which reaches ≈10 times deeper than the 5σ confusion limit in SPIRE. This provides more reliable star formation rates (SFRs), especially for the fainter galaxies, and hence a more reliable MS.
Methods. We built a pipeline that uses the spectral energy distribution (SED) modelling and fitting tool CIGALE to generate flux density priors in the Herschel SPIRE bands. These priors were then fed into the de-blending tool XID+ to extract flux densities from the SPIRE maps. In the final step, multi-wavelength data were combined with the extracted SPIRE flux densities to constrain SEDs and provide stellar mass (M⋆) and SFRs. These M⋆ and SFRs were then used to populate the SFR-M⋆ plane over 0.2 ≤ z < 6.0.
Results. No significant evidence of a high-mass turn-over was found; the best fit is thus a simple two-parameter power law of the form log(SFR) = α[log(M⋆) − 10.5] + β. The normalisation of the power law increases with redshift, rapidly at z ≲ 1.8, from 0.58 ± 0.09 at z ≈ 0.37 to 1.31 ± 0.08 at z ≈ 1.8. The slope is also found to increase with redshift, perhaps with an excess around 1.8 ≤ z < 2.9.
Conclusions. The increasing slope indicates that galaxies become more self-similar as redshift increases. This implies that the specific SFR of high-mass galaxies increases with redshift, from 0.2 to 6.0, becoming closer to that of low-mass galaxies. The excess in the slope at 1.8 ≤ z < 2.9, if present, coincides with the peak of the cosmic star formation history.
Pilot Study of Intra-Aortic Balloon Occlusion to Limit Morbidity in Patients with Adherent Placentation Undergoing Cesarean Hysterectomy
Authors: Blumenthal Elizabeth , Rao Rashmi , Murphy Aisling , Gornbein Jeffrey , Hong Richard , Moriarty John , Kahn Daniel , Janzen Carla 
Published: April 2018

Objective We study whether using an intra-aortic balloon (IAB) during cesarean hysterectomy decreases delivery morbidity in patients with suspected morbidly adherent placentation.
Study Design This is a retrospective cohort study of deliveries complicated by suspected abnormal placentation between 2009 and 2016 comparing maternal and neonatal outcomes with an IAB placed prior to cesarean hysterectomy versus no IAB. The primary outcome included quantified blood loss (QBL).
Results Thirty-five cases were reviewed, 16 with IAB and 19 without IAB. No difference was seen in median QBL between the two groups (1,351 vs. 1,397 mL; p = 0.90). There were no significant differences in overall surgical complications (19% IAB, 21% no IAB; p = 0.86), bladder complications (12 vs. 21%; p = 0.66), intensive care unit admissions (12 vs. 26%; p = 0.41), surgical duration (2.9 vs. 2.8 hour; p = 0.83), or blood transfusions (median 2 vs. 2; p = 0.27) between the two groups. There was one groin hematoma at the balloon site that was managed conservatively. There were no complications involving thrombosis or limb ischemia in the IAB group.
Conclusion While we did not detect statistically significant differences, larger studies may be warranted given the potential for extreme morbidity in these cases. This study highlights the potential use of an IAB in the management of these cases.
Reaktion der Endothelzellen auf kurzzeitig physiologisch erhöhte hydrostatische Drücke und oxidativen Stress in vitro
Authors: Mann Carolina , Thanos Solon , Brockhaus Katrin , Grus Franz H. , Pfeiffer Norbert , Prokosch Verena 
Published: April 2018

Zusammenfassung
Hintergrund Im Rahmen der Pathogenese des Glaukoms wird die endotheliale Dysfunktion zunehmend diskutiert. Peripapilläre Blutungen sind diagnostisch wegweisend. Die Korrelation von Glaukomerkrankungen mit vaskulärem Dysregulationssyndrom ist eindeutig. Ziel dieser Studie ist es, die genaue Reaktion der Endothelzellen auf erhöhten hydrostatischen und oxidativen Stress zu untersuchen.
Material und Methoden In vitro wurden primär dissoziierte BMECs (brain microvascular endothelial cells) für 3 Tage normalem und leicht erhöhtem hydrostatischem Druck von 60 und 120 mmHg in einer Druckkammer ausgesetzt. Zusätzlich wurden sowohl druckbelastete als auch nicht druckbelastete Zellen oxidativem Stress in Form von geringen Konzentrationen H2O2 ausgesetzt. Ein Live/Dead Assay wurde durchgeführt, um die Zellviabilität zu messen. Morphologisch wurden die Zellen mit immunhistochemischer Aktinfärbung beurteilt.
Ergebnisse Interessanterweise zeigten die Endothelzellen sowohl unter 60 mmHg als auch unter 120 mmHg kein vermehrtes Absterben im Vergleich zu den Zellen ohne Belastung. Auch morphologisch zeigten sich keine großen Unterschiede. Gegenüber oxidativem Stress wurden alle Zellen schon bei kleinen Mengen geschädigt. Keinen Unterschied konnte man zwischen oxidativem Stress ohne vorherige Druckbelastung und oxidativem Stress mit vorheriger Druckbelastung von 120 mmHg für 3 Tage feststellen.
Schlussfolgerung Wir konnten keinen direkten Effekt in Form von vermehrtem Zelluntergang der Endothelzellen auf erhöhten hydrostatischen Druck feststellen. Allerdings zeigt die Reaktion auf die geringen Konzentrationen von oxidativem Stress, dass die Zellen im Rahmen der Pathogenese des Glaukoms doch in Mitleidenschaft gezogen werden. Der oxidative Stress scheint hier eine besondere Rolle zu spielen.