bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comparison of Routine [68Ga]Ga-PSMA-11 Preparation using Locametz and Illuccix Kits
Authors: Wang Ivan E. , Morrissette Luke J. , Wong Ka Kit , Brooks Allen F. , Dakanali Marianna , Scott Peter J. H. 
Published: December 2024

Abstract

Background Approval of Locametz and Illuccix kits for the manufacture of [68Ga]Ga-PSMA-11 (gallium Ga68 gozetotide), a PET imaging agent for prostate cancer, as well as the corresponding therapeutic ([177Lu]Lu-PSMA-617 Pluvicto), has led to a rapid increase in demand for [68Ga]Ga-PSMA-11 PET imaging. Radiopharmaceutical manufacturers, using 68Ge/68Ga generators, may decide to adopt Locametz and/or Illuccix kits, which requires a comparison to select the most suitable kit for day-to-day use. The objective of this article is to compare both kits and provide guidance for selecting one for routine use, as well as evaluate labeling consistency of both kits during routine production. Additionally, we report our experience during 1.5 years of daily [68Ga]Ga-PSMA-11 production at our facility using both kits.Results Locametz (n = 181) and Illuccix (n = 256) kits were prepared using non-silicone coated and silicone-coated needles with 68Ga activities ranging from 0.53 to 3.16 GBq, with a failure rate of 1 in 128 runs for both kits. With Locametz, a 3.7 GBq generator and 10-min incubation at room temperature gave doses that passed quality control (QC) testing. Use of non-silicone coated needles in the process led to solution discoloration, and QC failure. Additionally, lack of vial inversion led to inconsistent labeling, which improved with subsequent vial agitation. For Illuccix, addition of the acetate buffer to the precursor vial prior to adding the [68Ga]GaCl3 simplifies the workflow. The maximum tolerated activity was 1.85 GBq. Lack of vial inversion led to failures, which were rectified by agitating the vial to properly incorporate the acetate solution with the generator eluate.Conclusions Both kits benefited from using a syringe pump to elute the 68Ge/68Ga generator, vial agitation, and longer length/smaller bore silicone coated needles. Both kits have similar workflows, comparable QC outcomes, and result in equivalent clinical images. Thus, the decision between kits will ultimately be determined on production preferences. Since radiopharmacies have an established “kit-based” workflow, Locametz kits with higher allowed activities and longer shelf-life may offer benefits. Conversely, more traditional PET manufacturing facilities might benefit from using Illuccix kits due to compatibility with cyclotron-produced [68Ga]GaCl3 allowing for kit batching. Ultimately, the commercial availability of 2 approved kits for production of [68Ga]Ga-PSMA-11 PET has facilitated ready access to this important new imaging agent.
Analysis of Water Inflows and Withdrawals at the Bagré Dam within the Energy-Water-Food Nexus
Authors: Oueadrogo Yasmina , Yamegueu Daniel , TAZEN FOWE , Leye Babacar , Bologo/Traore Maimouna 
Published: December 2024

Abstract

The energy-water-food nexus highlights the diverse and complex interrelationships between these three resources. The impact of climate change on water resources can disrupt hydroelectricity and agricultural production. A better understanding of these impacts is important in order to find appropriate solutions. The objective of this study is to assess the potential effects of climate change on water inflows, storage, and withdrawals from the Bagré dam in Burkina Faso. This dam is multipurpose, particularly for agricultural and hydropower production. To achieve this, a model has been developed using the Random Forest approach to assess future climate change in the Bagré area and its impact on water inflows and withdrawals for the dam’s uses. The results show a temperature rise of 1°C to 5°C and a general increase in rainfall of up to 50% for the MeanEns model over future periods compared with the reference period. These changes in climate variables result in an increase in dam inflows of up to 300 Mm3 compared with the reference period, an increase in water storage of up to 400 Mm3, and an increase in agricultural and hydroelectric water volumes of up to 8 and 40 Mm3 respectively. The performance of the model built is quite satisfactory for simulating water inflows and water withdrawals for irrigation and hydroelectricity. In view of the increase in the various hydroelectric and agricultural water requirements, it is important to find effective ways for efficient water resource management of the Bagré dam.
Relationship between resilience and caregiver burden among parents of children with epilepsy: Chain-mediating effect of hope and subjective well-being
Authors: Zhang Shasha , Zhang Fang , Yao Wenying , Sun Lihua , Yang Tingting , Zhu Li , Wei Wenjun , Fang Wanyue 
Published: December 2024

Abstract

Objectives:This study aimed to investigate the mediating roles of hope and subjective well-being in the effect of parental resilience on caregiver burden among children with epilepsy. Our findings can provide novel insights for alleviating the caregiver burden experienced by the parents of children with epilepsy.
Methods:From September 2023 to May 2024, this cross-sectional study surveyed 415 parents of children with epilepsy at three children’s hospitals in Suzhou City, China. We recorded data on the Zarit Caregiver Burden Interview (ZBI), the Connor-Davidson Resilience Scale (CD-RISC), the Herth Hope Index (HHI), and the Index of Well-being (IWB) using self-reported questionnaires.
Results: Caregiver burden was negatively associated with resilience (P<0.001), hope (P<0.001), and subjective well-being (P<0.001). Resilience indirectly affected caregiver burden through three mediating pathways, including hope (effect=-0.194), subjective well-being(effect=-0.142), and a combination of hope and subjective well-being (effect=-0.052). They accounted for 23.66%, 17.32%, and 6.34% of the total effect, respectively.
Conclusion:We revealed the multiple mediating effects of hope and subjective well-being on the role of resilience in caregiver burden. Medical staff should pay more attention to the parents of children with epilepsy who have lower resilience and actively take corresponding measures to promote their hope levels, enhance their subjective well-being, and reduce the burden of care.
Exploring Burnout in Medical Education: A Mixed-method Study Among University of Ibadan Medical Students
Authors: Adesola Adeniyi Abraham , Akoki David Mobolaji , Aderemi Temilola Victoria , Fola-Oyetayo Olorunyomi Chisom , Asogwa Chukwuebuka Stanley , Ojile Moses Ojomakpenen , Ijezie Ifeanyichukwu Collins 
Published: December 2024

Abstract

Introduction: Medical students face intense academic pressures leading to burnout, a critical yet overlooked challenge in medical education. This study explores burnout prevalence and its contributing factors among medical students at the University of Ibadan, Nigeria, examining its extent and broader implications.
Methods: A mixed-method cross-sectional study combined a self-administered online survey and focus group discussions (FGD). The Oldenburg Burnout Inventory for Students (OLBI-S) measured burnout levels, while the Multidimensional Scale of Perceived Social Support (MSPSS) assessed perceived social support. Institutional support service availability was also evaluated. The survey achieved a 51% response rate (355 participants), with 11 randomly selected students participating in FGDs. SPSS analysed quantitative data, while FGD transcripts underwent thematic analysis.
Results: The study revealed an 81.1% burnout prevalence rate, showing significant associations with gender (p<0.001) and academic year (p=0.004). Of the participants, 40.3% were unaware of the College of Medicine Support Service, while only 3.9% had utilised it. Major burnout contributors included overbearing workload, insufficient breaks, and physical stressors.
Conclusion: The high burnout rates discovered at Nigeria's pioneering medical school have profound implications for medical education nationwide. These findings emphasise the need for culturally attuned, comprehensive interventions, including curriculum reforms and enhanced student support services, to mitigate burnout and improve student well-being.
The effect of the position of the femoral endobutton on knee function in the posterior cruciate ligament reconstruction: a retrospective study
Authors: Xu Libin , Lou Fangyong , Jiang Haitao 
Published: December 2024

Abstract

Background
To investigate the effect of the position of the femoral endobutton on knee function in the posterior cruciate ligament reconstruction (PCLR).
Methods
A retrospective study was conducted to analyze 96 patients who underwent single-bundle reconstruction of posterior cruciate ligament (PCL) in our hospital from January 2020 to June 2023. The patients were divided into three groups according to the position of the endobutton on the lateral knee radiography. The first group is the position of endobutton in front of the lateral cortex of the femur, the second group is the position of endobutton in back of the lateral cortex of the femur, and the third group is the position of endobutton behind the femur. Knee Lysholm score, International Knee Documentation Committee (IKDC) Evaluation, Tegner Activity Scale, knee flexion angle and postoperative complication rate of the three groups were compared to evaluate whether there were statistical differences among the three groups.
Results
There were 39 patients in group 1, 46 patients in group 2, and 11 patients in group 3. The mean Lysholm Knee Scores of the group 1 was 91.77, 92.17 in group 2, and 90.36 in group 3. The mean IKDC Evaluation of group 1 was 90.48, 92.41 in group 2, and 93.00 in group 3. Tegner Activity Level was 5.69 in group 1, 5.72 in group 2, and 5.45 in group 3. The mean flexion degree was found as 125° in patients in group1, 127° in group 2, and 122° in group 3. There was no statistically significant difference between Lysholm Knee Scores (p = 0.434), IKDC (p = 0.068), Tegner Activity Level (p = 0.797), and knee flexion angle (p = 0.135). There was also no significant difference in the incidence of complications among the three groups (p > 0.05).
Conclusion
There were not statistically differences in clinical functional results when comparing patients’ endobutton location on femur. This indicates that it does not need to adjust the orientation of the exit hole of the femur whether it is forward or backward during the PCLR.
Predicting Breast Cancer Prognosis Using PR and PIK3CA Biomarkers: A Comparative Analysis of Diagnostic Groups
Authors: Feng Yuting , Yang Mengqin , Song Qingzhen , Bu Peng , Lian Jing 
Published: December 2024

Abstract

Purpose
To evaluate the prognostic significance of progesterone receptor (PR) expression and the PIK3CA mutation status in HR+/HER2 − breast cancer patients, with the goal of screening patients who may derive the greatest benefit from PI3K-targeted therapy.
Methods
A retrospective analysis was conducted on HR+/HER2 − breast cancer patients stratified by PR expression levels and PIK3CA mutation status. The study population was divided into groups on the basis of a median PR threshold of 50% and further subdivided by PIK3CA mutation status. To evaluate the variability of clinicopathologic features among these groups, t tests and ANOVA were employed. The influence of these variables on survival was analyzed via Cox regression. Additionally, a risk prediction model was developed using the PR expression level and PIK3CA mutation status. The prognostic utility of this model was examined via both Kaplan‒Meier (KM) survival curves and receiver operating characteristic (ROC) analyses. These methods have also been utilized to explore the associations between clinicopathologic parameters and clinical outcomes with respect to survival prediction and prognosis.
Results
Significant differences in age, ER expression, and Ki67, HER2, and PIK3CA mutation status were detected between the groups (P < 0.05). Specifically, elevated PR expression was correlated with lower levels of Ki67 and low HER2 expression. The presence of a PIK3CA mutation was significantly linked to survival outcomes according to both univariate and multivariate Cox regression analyses. Moreover, ROC analysis revealed that models incorporating both PR expression and PIK3CA mutation status achieved the highest level of diagnostic precision (AUC = 0.82).
Conclusion
PR expression and PIK3CA mutation status are significant predictors of prognosis in HR+/HER2 − breast cancer patients. These findings suggest that assessing these biomarkers in conjunction can be instrumental in identifying patients who stand to gain the most from PI3K-targeted therapies, leading to more personalized treatment strategies. This innovative approach ensures that treatments are not only aligned with the unique genetic profile of each tumor but also potentially increase the efficacy and outcome of the therapies administered.
Social capital, health status, and sociodemographic factors associated with subjective well-being among older Adults: a comparative study of community dwellings and nursing homes
Authors: Chen Yan , Wang Dahui , Chen Wenhao , Zhao EN Xi , Li Wanjing , Zhu Shanshan , Wu Xianlan 
Published: December 2024

Abstract

Background: This study aimed to examine the differences in relationships among social capital components, health status, sociodemographic characteristics, and subjective well-being (SWB) among older adults in institutionalized versus non-institutionalized care environments.
Methods: A cross-sectional survey was conducted involving 1,037 older adults aged 65-95 years from nine communities and nine nursing homes across three regions of Zhejiang Province, China. Social capital and SWB were assessed using the Social Capital Scale and the Memorial University of Newfoundland Scale of Happiness (MUNSH), respectively. Propensity score matching (PSM, 1:1, caliper width 0.02) was applied to balance key sociodemographic characteristics and health status between community-dwelling and nursing home residents. Multiple linear regression was utilized to analyze the relationships among social capital components, health status, sociodemographic factors, and SWB in both groups.
Results: PSM identified 290 older adults in community dwellings and a comparable group (n = 290) in nursing homes. Comparative analysis showed that nursing home residents demonstrated lower SWB. Multiple linear regression revealed that social connection, trust, and cohesion positively associated with SWB in both groups. However, social participation was significantly linked only with community dwellings residents. Both groups showed a positive relationship between SWB and self-rated health, but no association was found with the number of chronic conditions. Additionally, higher income (≥3000 RMB) and education level (middle) were linked to increased SWB among community-dwelling older adults, whereas family structure—specifically, having no partner and three or more children—negatively impacted SWB in the nursing home group.
Conclusion: Social capital and health status showed a strong and consistent association with SWB in both groups. Strengthening social connections, trust, and cohesion, along with maintaining positive health perceptions, is expected to enhance the well-being of older adults, particularly for those in institutional settings. Notably, differences in how sociodemographic factors influence SWB across settings. These findings indicate the necessity for tailored interventions that address the unique needs of each care environment to promote healthier aging experiences.
PEDV infection induces ferroptosis in Vero cells via an ACSL-mediated lipid peroxidation pathway
Authors: Weng Qian , Li Yuheng , Wei Yuze , Wang Simin , Hu Tingyu , Pei Zhihua , Wang Kai , Hu Guixue 
Published: December 2024

Abstract

Porcine epidemic diarrhea virus (PEDV) is a highly contagious viral pathogen causing severe economic losses in the swine industry. However, the underlying mechanisms of PEDV-induced host cell death largely unknown. In this study, we investigated the role of ferroptosis, a non-apoptotic form of programmed cell death, in PEDV pathogenesis. The experiments were divided into four groups: a control group, a PEDV (MOI = 1.0) group, an Erastin (5µM) positive control group and a Liprostatin (0.5µM) negative control group. Levels of GSH, ROS, Fe3+ and cell viability were evaluated using ELISA test kits. Fluorescence microscopy was employed to assess Fe2+ aggregation, while flow cytometry was utilizeed to measure lipid peroxide levels. The mRNA transcript levels of key gene involved the ferroptosis pathway-ACSL4, GPX4, ALOX15 and LPCAT3 - were determined by quantitative reverse transcription PCR. Compared to the control group, the PEDV group exhibited a significant decrease in GSH levels (P < 0.01) and a gradual reduction in Fe3+ levels (P < 0.05) over time. Furthermore, the PEDV group showed a substantial increase in ROS release (P < 0.05) and a corresponding decrease in cell viability (P < 0.05) relative to the control group. The results of the qRT-PCR revealed that the expression levels of ACSL4, ALOX15 and LPCAT3 mRNA were significantly elevated in the PEDV group (P<0.01). Additionally, Western blot analysis confirmed that the protein expression levels of ACSL4, ALOX15 and LPCAT3 also increased progressively (P < 0.01). In conclusion, our findings demonstrated that PEDV can induce ferroptosis in Vero cells through the lipid peroxidation pathway mediated by ACSL 4.
Optimising Coil Design Based on Sensitivity Analysis of Magnetic Force Induced Between Misaligned Coil Pairs
Authors: Yang Yuxin , Robertson Will , Jafari Azadeh , Arjomandi Maziar 
Published: December 2024

Abstract

Cylindrical coils are widely used components in several modern technologies, and achieving the precise coaxial alignment condition for cylindrical coil positioning or installation is difficult due to inherent manufacturing errors. The lateral force induced by coil misalignment significantly impacts overall operational efficiency of systems, such as maglev systems applied in wind tunnels for drag measurement. Therefore, understanding the sensitivity of lateral force to misalignment in a coil-formed system is critical. In this study, multiple analytical methods are adopted to find the relationships between magnetic forces, coil misalignment, and coil geometry. A nondimensionalized misalignment parameter is introduced and used to investigate the sensitivity of magnetic force in both vertical and lateral directions to the variation of coil misalignment. In addition, coils with different geometry configurations were modelled and analysed under identical power input, showing the influence of geometric variations on the magnetic force sensitivity. Based on the findings of the sensitivity analysis, distinct strategies for coil design and selection are proposed respectively for static maglev systems and systems involving translational motion of objects.
Deep Learning for Motion Classification in Ankle Exoskeletons Using Surface EMG and IMU Signals
Authors: Estévez Silas Ruhrberg , Mallah Josée , Kazieczko Dominika , Tang Chenyu , Occhipinti Luigi Giuseppe 
Published: December 2024

Abstract

Ankle exoskeletons have garnered considerable interest for their potential to enhance mobility and reduce fall risks, particularly among the aging population. The efficacy of these devices relies on accurate real-time prediction of the user’s intended movements through sensor-based inputs. This paper presents a novel motion prediction framework that integrates three Inertial Measurement Units (IMUs) and eight surface Electromyography (sEMG) sensors to capture both kinematic and muscular activity data. A comprehensive set of activities, representative of everyday movements in barrier-free environments, was recorded for the purpose. Our findings reveal that Convolutional Neural Networks (CNNs) slightly outperform Long Short-Term Memory (LSTM) networks on a dataset of five motion tasks, achieving classification accuracies of 96.5°æ0.8% and 87.5°æ2.9%, respectively. Furthermore, we demonstrate the system’s proficiency in transfer learning, enabling accurate motion classification for new subjects using just ten samples per class for finetuning. The robustness of the model is demonstrated by its resilience to sensor failures resulting in absent signals, maintaining reliable performance in real-world scenarios. These results underscore the potential of deep learning algorithms to enhance the functionality and safety of ankle exoskeletons, ultimately improving their usability in daily life.
Mathematical insights into cancer-immune dynamics modulated by immunotherapy and interleukins
Authors: Boudjellal Laid , Soares Ana Jacinta , Torres Maria Joana 
Published: December 2024

Abstract

In this work, we develop a new mathematical model to describe the tumor-immune system interactions in the presence of interleukins and a recently developed immunotherapy. We describe the tumor-immune interactions at the cellular level, using the approach of the Kinetic Theory of active particles, by considering four populations, namely tumor cells, activated immune cells, immune cells and interleukins.We also derive the macroscopic model relative tothe kinetic system and study the well-posedness of the Cauchy problem for thecorresponding system of equations.Then, we determine the equilibrium states of the system and investigate their asymptotic stability. Particular attention is devoted to the global stability of a biologically meaningful tumor-free equilibrium.Some numerical simulations are also performed in view of illustrating the behaviour of the solutionpredicted by the theoretical analysis.
AMS Subject Classification: 82C40, 92B05, 92-10, 37N25.
The Role of a Novel TRIP12 Mutation in Intellectual Disability: A Molecular and Clinical Investigation one big Azeri family from Iran
Authors: Nobakht Ramiz , Arish Sara , Hasanzadeh Shirin , Mokabber Haleh , davarnia Sana , Davarnia Behzad 
Published: December 2024

Abstract

Thyroid Hormone Receptor Interactor 12 (TRIP12; MIM #617752) is an autosomal dominant hereditary disorder involved in the ubiquitin fusion degradation pathway and the regulation of DNA damage-induced chromatin ubiquitination. Positioned on chromosome 2 at position 2q36.3, TRIP12 is a member of the E3 ubiquitin ligase family. This gene plays a vital role in proteasomal degradation by catalyzing substrate ubiquitination and regulating processes such as cell cycle progression, DNA damage repair, and chromatin remodeling. Mutations in TRIP12 can result in intellectual disability (ID), Clark-Baraitser syndrome, and various physical and behavioral abnormalities. The proband, a 32-year-old male, exhibited intellectual disability, delayed speech, and behavioral abnormalities without autistic spectrum disorders. The novel TRIP12 variant was detected through WES and validated by Sanger sequencing in affected family members. In silico tools predicted the deleterious effect of the variant, and protein modeling indicated significant structural changes. RT-qPCR demonstrated increased TRIP12 mRNA levels, suggesting a compensatory mechanism for decreased protein stability. This study examines the role of the TRIP12 gene in the ubiquitin pathway and associated pathologies such as intellectual disability and developmental delay.