bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parental Acceptance of Fetal Tissue Donation
Authors: Dawood Yousif , van den Hoff Maurice J. B. , Ravelli Anita C. J. , de Bakker Bernadette S. , Pajkrt Eva 
Published: November 2024

ImportanceHuman fetal tissue is essential for biomedical research, providing unparalleled insights into human development and disease.ObjectiveTo assess changes in parental decisions to donate fetal tissue following termination of pregnancy after the introduction of the Dutch Fetal Biobank (DFB) and to identify factors associated with consent to donate.Design, Setting, and ParticipantsThis cohort study collected data from all individuals assigned female at birth (hereafter referred to as participants) who underwent a termination of pregnancy at the Amsterdam University Medical Center from January 1, 2008, to December 31, 2022. No exclusion criteria were applied.ExposureIntroduction of the DFB on September 1, 2017, which offers the option to donate fetal tissue after pregnancy termination.Main Outcome and MeasureRates of fetal tissue donation before and after DFB introduction and associations between patient characteristics (maternal age, race and ethnicity, socioeconomic status, gestational age, and reason for termination) and consent to donation were assessed using multivariable binary logistic regression modeling.ResultsOf a total of 1272 participants (mean [SD] age, 33.0 [5.4] years), 576 (45.3%) were nulliparous. The mean (SD) gestational age at termination was 18 weeks 3 days (26 days), and reasons for termination were primarily because of structural defects (567 participants [44.6%]), with only a small portion (58 participants [4.6%]) terminating for social reasons (eg, unwanted or unplanned pregnancy). Fetal tissue donations increased from 1.2% (8 donations among 663 terminations) before the DFB introduction to 21.7% (132 donations among 609 terminations) after its introduction. This rise was primarily due to a shift from collective cremation to donation, while individual cremation or burial rates remained stable. The consent rate was 30.3% (132 of 436) for participants informed about the donation option. No significant demographic differences were found between participants who consented and those who did not, except for gestational age; consent rates decreased with advanced gestational age (odds ratio per week, 0.88; 95% CI, 0.83-0.94). Higher consent rates were observed for terminations due to social reasons (odds ratio, 3.56; 95% CI, 1.40-9.10).Conclusions and RelevanceThese findings suggest that a substantial proportion of individuals may be willing to donate fetal tissue for biomedical research after pregnancy termination. Integrating donation options in posttermination counseling respects patient autonomy and could ethically increase tissue availability for research. Expanding biobanks and fostering international collaboration is crucial for standardizing practices and ensuring equitable research benefits.
Investigation of Solid‐State Interface Reactions Between the Compositionally Complex Solid Solution Noble Metal Alloy Ag‐Au‐Pd‐Pt and Si
Authors: Li Yujiao , Kostka Aleksander , Savan Alan , Ludwig Alfred 
Published: November 2024

AbstractStudying the stability of polyelemental systems such as compositionally complex solid solutions (CCSS) on the atomic scale against thermal decomposition or reaction with other elements is necessary to evaluate their suitability for applications. For this, the combinatorial processing platform approach is used to report on the stability of an exemplary noble metal CCSS thin film system (Ag‐Au‐Pd‐Pt). The thin film is sputter‐deposited at room temperature on flat and pre‐sharpened Si microtip array surfaces, protected solely by their native oxide. The as‐deposited film is a fcc solid solution which is subjected to sequential annealing up to 400 °C. Up to 300 °C, the native Si oxide is a sufficient reaction barrier. However, after annealing at 400 °C solid‐state reactions between the noble metal film and the Si substrate occur, forming mainly a ternary Pd‐Pt‐Si silicide with a crystal structure of Pd₂Si (space group P6̅m2), as revealed by a semi‐correlative APT/TEM analysis. Removal of Pd and Pt from the thin film causes partial decomposition of the initial fcc solid solution, with the formation of a Ag‐Au‐rich phase. Moreover, the interaction of the quaternary alloy system with Si is compared to that of pure Pt/Pd with Si. From this, information of preferential silicide formation is derived.
Piezo1 Regulates Stiffness‐Dependent DRG Axon Regeneration via Modifying Cytoskeletal Dynamics
Authors: Lei Mengshi , Wang Weiyou , Zhang Hong , Gong Jihong , Cai Hanmian , Wang Zhili , Zhu Le , Yang Xiaofei , Wang Shen , Ma Cong 
Published: November 2024

AbstractDespite medical interventions, the regenerative capacity of the peripheral nervous system is limited. Dorsal root ganglion (DRG) neurons possess the capacity to detect mechanical signals from their microenvironment, but the impact and mechanism by which these signals regulate axon regrowth and even regeneration in DRG neurons remain unclear. In this study, DRG neurons from newborn rats are cultured on substrates with varying degrees of stiffness in vitro to investigate the role of mechanical signals in axon regrowth. The findings reveal that substrate stiffness plays a crucial role in regulating axon regrowth, with an optimal stiffness required for this process. In addition, the data demonstrate that Piezo1, a mechanosensitive cation channel, detects substrate stiffness at the growth cone and regulates axon regrowth through activating downstream Ca2+–CaMKII–FAK–actin cascade signaling pathway. Interestingly, knocking down Piezo1 in adult rat DRG neurons leads to enhanced axon regeneration and accelerated recovery of sensory function after sciatic nerve injury. Overall, these findings contribute to the understanding of the role of mechanical signals in axon regeneration and highlight microenvironmental stiffness as a promising therapeutic target for repairing nerve injuries.
Electrical Microneedles for Wound Treatment
Authors: Wang Yu , Cai Lijun , Fan Lu , Wang Li , Bian Feika , Sun Weijian , Zhao Yuanjin 
Published: November 2024

AbstractElectrical stimulation has been hotpot research and provoked extensive interest in a broad application such as wound closure, tissue injury repair, and nerve engineering. In particular, immense efforts have been dedicated to developing electrical microneedles, which demonstrate unique features in terms of controllable drug release, real‐time monitoring, and therapy, thus greatly accelerating the process of wound healing. Here, a review of state‐of‐art research concerning electrical microneedles applied for wound treatment is presented. After a comprehensive analysis of the mechanisms of electrical stimulation on wound healing, the derived three types of electrical microneedles are clarified and summarized. Further, their applications in wound healing are highlighted. Finally, current perspectives and directions for the development of future electrical microneedles in improving wound healing are addressed.
Spherical DNA Nanomotors Enable Ultrasensitive Detection of Active Enzymes in Extracellular Vesicles for Cancer Diagnosis
Authors: Deng Jinqi , Zhao Shuai , Xie Kai , Liu Chao , Sheng Chuangui , Li Junhong , Dai Bo , Wan Shuo , Li Lele , Sun Jiashu 
Published: November 2024

AbstractEnzymes encapsulated in extracellular vesicles (EVs) hold promise as biomarkers for early cancer diagnosis. However, precise measurement of their catalytic activities within EVs remains a notable challenge. Here, we report an enzymatically triggered spherical DNA nanomotor (EDM) that enables one‐pot, cascaded, and highly sensitive analysis of the activity of EV‐associated or free apurinic/apyrimidinic endonuclease 1 (APE1, a key enzyme in base excision repair) across various biological samples. The EDM capitalizes on APE1‐triggered activation of DNAzyme (Dz) and its autonomous cleavage of substrates to achieve nonlinear signal amplification. Using EDM, we demonstrate a strong correlation between APE1 activity in EVs and that of their parental cancer cells. Additionally, EV APE1 mirrors the fluctuation of cellular APE1 activity in response to chemotherapy‐induced DNA damage. In a pilot clinical study (n=63), the EDM‐based assay reveals that more than 80 % of active APE1 in serum samples is EV‐encapsulated. Notably, EV APE1 can differentiate early prostate cancer (PCa) patients from healthy donors (HDs) with an overall accuracy of 92 %, outperforming free APE1 in sera. We anticipate that EDM will become a versatile tool for quantifying EV‐associated enzymes.
Rebuilding Peripheral F, Cl, Br Footprints on Acceptors Enables Binary Organic Photovoltaic Efficiency Exceeding 19.7 %
Authors: Cao Xiangjian , Wang Peiran , Jia Xinyuan , Zhao Wenkai , Chen Hongbin , Xiao Zheng , Li Jiaqi , Bi Xingqi , Yao Zhaoyang , Guo Yaxiao , Long Guankui , Li Chenxi , Wan Xiangjian , Chen Yongsheng 
Published: November 2024

AbstractGiven homomorphic fluorine (F), chlorine (Cl) and bromine (Br) atoms are featured with gradually enlarged polarizability/atomic radius but decreased electronegativity, the rational screen of halogen species and locations on small molecular acceptors (SMAs) is quite essential for acquiring desirable molecular packing to boost efficiency of organic solar cells (OSCs). Herein, three isomeric SMAs (CH−F, CH−C and CH−B) are constructed by delicately rebuilding peripheral F, Cl, Br footprints on both central and end units. Such a re‐permutation of peripheral halogens could not only maintain the structural symmetry of SMAs to the maximum, but also acquire extra asymmetric benefits of enhanced dipole moment and intramolecular charge transfer, etc. Moreover, central brominating enhances molecular crystallinity of CH−B without introducing undesirable steric hindrance on end groups, thus rendering a better balance between high crystallization and domain size control in PM6:CH−B blend. Further benefitting from the large dielectric constant, small exciton binding energy, optimized molecular packing and great electron transfer integral, CH−B affords the first class binary OSC efficiency of 19.78 %, moreover, the highest efficiency of 18.35 % thus far when increasing active layer thickness to ~300 nm. Our successful screening in rebuilding peripheral halogen footprints provides the valuable insight into further rational design of SMAs for record‐breaking OSCs.
Design of cellulose nanocrystal‐integrated poly(vinyl alcohol)/polyethylene glycol electrospun nanofiber scaffolds for biomedical applications
Authors: Nasalapure Anand V. , Pattadkal Shrishail , Ghatti Vanita , Chapi Sharanappa , Kumarswamy Yogesh Kumar , Kasai Deepak R. , Chalannavar Raju K. 
Published: November 2024

AbstractThe present study aims to fabricate cellulose nanocrystals (CNCs) doped poly(vinyl alcohol) polyethylene glycol (PVA/PEG) nanocomposite films for biomedical applications. The nanocomposite films were prepared by using electrospinning techniques with varying percentages of CNCs (10% and 20%) mixed with PVA and PEG (50:50). The properties of the produced nanocomposites were evaluated using a battery of tests. The results of surface morphology and structural miscibility obtained from field emission‐scanning electron microscopy (FE‐SEM), atomic force microscopy, and Fourier transformer infra‐red studies revealed appreciable compatibility among the three components (CNCs, PVA, and PEG). FE‐SEM micrographs revealed that the nanomaterials underwent a dramatic transition from discontinuous to continuous fibers, with interconnected or network‐like fibers. The constructed scaffold material's stability went up from three to four phases, according to thermal and degradation experiments. In addition, research on biodegradation and water absorption found that CNCs outperformed pure PVA and PVA/PEG in terms of swelling percentage time and enzyme degradation rate. The hemolysis assay of the PVA/PEG/CNC scaffold showed good compatibility (below 5%) and according to the MTT assay, both NIH 3T3 and L929 cells survived well, indicating that the CNCs could serve as a useful scaffold for tissue engineering applications such skin tissue regeneration. In sum, the findings proved that biological domains including tissue engineering and wound healing can benefit from produced nanofiber scaffolds.
A new green rigid polyurethane foam based on modified palm oil: Preliminary study of their potential for marine buoy applications
Authors: Sukhawipat Nathapong , Kongprabat Thansiri , Uthaipan Nattapon , Saetung Anuwat , Saetung Nitinart 
Published: November 2024

AbstractThis study demonstrates a new green rigid polyurethane (PU) based on modified palm oil (MPO) for marine buoy applications. The bio‐polyol based on palm oil was synthesized using hydrogen peroxide and formic acid. The foam formation was by kinetic foaming reaction. The MPO‐based PU foams made with increased water content had closed cells that grew larger with water content to provide a low‐density foam. The resistance to flammability of PU foam was improved by adding triphenyl phosphate (TPP). The PU foam with increased TPP content had enlarged cells, increased water absorption and decreased compressive strength. In addition, carbon black (CB)‐containing foam had uniform, large foam cells, comparatively high density, higher compressive strength, and low water absorption. Moreover, a high CB content gave shorter extinguishing time for PU foam. The PU foams were investigated for buoyancy and compared with a commercial PU foam buoy. The commercial buoy had lower density, providing better‐floating performance than PU foam. Interestingly, CB_8 had an elevated density, but low water absorption rate, similar to the commercial buoy PU foam. The investigations showed that the PU foam from MPO is eco‐friendly, and the buoyancy performance of that foam is improved on adding TPP or CB.
A Hemicyanine‐Based Highly Sensitive and Selective Near‐Infrared Fluorescent Probe for Fe3+ in Aqueous Media
Authors: Ye Jia‐Hai , Zhang Yingming , Zhu Qiang , Chang Zhijian , He Weijiang 
Published: November 2024

ABSTRACTFerric ion is widely distributed in human cells and an important component of hemoglobin, which can promote the transportation of blood in the human body. Its ability to bind with oxygen is essential for participating in oxidation reactions and enzymatic reactions. Deficiency of iron (III) and excessive accumulation of iron in the blood can cause many health problems in the body, such as anemia, loss of appetite, fibrosis, reduced work routines, and decreased immunity. As a fluorescent dye, cyanine has the advantages of high fluorescence quantum yield, good optical and chemical stability, excitation and emission wavelengths in the near‐infrared region, high molar extinction coefficient, and small influence on pH variation. It is a fluorescent dye that modern scholars choose more. A new near‐IR hemicyanine derivative bearing 1,3‐dithiane moiety was developed as an efficient fluorescence probe 1 for the detection of Fe3+ in aqueous medium (THF/H2O, 1:1, v:v). This near‐IR fluorescence probe 1 exhibits high sensitivity and selectivity toward Fe3+ sensing with detection limit of 0.5 μM under lager scope of pH value (pH = 2–12).
Fecal microbiota transplantation for the treatment of intestinal and extra‐intestinal diseases: Mechanism basis, clinical application, and potential prospect
Authors: Yi Dongxin , Li Tao , Xiao Yuji , Zhang Xue , Hao Qiangqiang , Zhang Feng , Qiu Tianming , Yang Guang , Sun Xiance , Dong Ying , Wang Ningning 
Published: November 2024

AbstractTo review the theoretical basis and therapeutic effects of fecal microbiota transplantation (FMT) in various diseases in animal experiments and clinical studies, as well as the limitations and current standards of FMT application. PubMed and Web of Science databases were searched for articles published only in English between 1975 and 2023 on reliable results of animal experiments and clinical treatment of FMT. The properties of the gut microbiota and its interactions with the host metabolism are critical to human health, and microbiome disturbance is closely associated with human intestinal and extra‐intestinal diseases. Therefore, therapeutic tools targeting on the modulation of gut microbiota have attracted increasing attention, among which FMT represents the most widely studied intervention strategy. This review gathered and summarized application of FMT in intestinal diseases, metabolic diseases, hypertension, cancer, nervous system diseases and arthritis, and elaborated the beneficial effects that can be achieved by altering the microbiota with FMT and the mechanisms of action. In addition, the potential risks and side effects of FMT approach are discussed, as well as current efforts to standardize the development of FMT. Through a systemic review of the outcome and mechanism of FMT in the treatment of intestinal diseases and extra‐intestinal diseases, we aimed to provide a theoretical basis for the construction of an optimized FMT framework, so as to better exert its application prospects.
Impact of deep eutectic solvent deamidation on the solubility and conformation of rice dreg protein isolate
Authors: Shahid Asifa , Haq Muhammad Abdul , Hussain Naveed , Nawaz Haq , Xu Jiaxing 
Published: November 2024

AbstractBackground and ObjectivesRice dreg protein isolate (RDPI) is identified as a sustainable protein source with promising applications in the food and pharmaceutical industries. However, its utility is limited by poor solubility and restricted conformational stability. The objective of this study is to explore how natural deep eutectic solvent (NADES) deamidation can enhance the solubility and structural properties of RDPI, making it more suitable for various applications.FindingsThe study employed a two‐step process to obtain RDPI via isoelectric precipitation, followed by deamidation using an NADES made from glucose and citric acid. The deamidation process significantly improved RDPI's solubility, which enhanced its dispersibility in aqueous systems. Additionally, deamidated RDPI exhibited enhanced foaming and emulsion capacities, indicating improved functional properties due to increased structural flexibility.ConclusionsThe deamidation of RDPI using NADES effectively enhances both its solubility and functional properties, making it a more versatile ingredient for food and pharmaceutical applications. The improved structural flexibility achieved through deamidation is key to these enhancements.Significance and NoveltyThis research introduces NADES deamidation as a promising, sustainable approach for improving the solubility, conformational properties, and functional performance of RDPI. The findings provide valuable insights for the development of protein‐based ingredients with enhanced functionality, broadening their application potential in various industries.
Dodecylamine‐Assisted Hydrothermal Synthesis of Carbon‐Supported Ultrafine IrRu Nanoparticles for Oxygen Evolution Electrocatalysis and Overall Water Splitting
Authors: Huang Li , Ma Mengyuan , Liu Hui , Chen Dong , Xu Lin , Tian Shaonan , Yan Mei , Yang Jun 
Published: November 2024

AbstractRuthenium (Ru)‐ and iridium (Ir)‐based nanomaterials have always been regarded as efficient electrocatalysts for oxygen evolution reaction (OER) in acidic electrolytes. Herein, we develop a facile dodecylamine‐assisted hydrothermal synthesis for producing carbon‐supported IrRu alloy nanoparticles with controllable Ir/Ru ratios and ultrafine sizes towards high‐efficiency OER and overall water electrolysis. In this strategy, the dodecylamine that serves as a capping and reducing agent enables the final IrRu alloy nanoparticles to possess average sizes <3 nm and high degree of dispersion on carbon substrate. By combining high OER activity of Ru with high acidic robustness of Ir, the as‐prepared IrRu/C nanoparticles at a suitable Ir/Ru ratio of 1/3 show good activity and durability for the OER electrocatalysis and overall water splitting. In specific, the Ir1Ru3/C catalyst exhibits the lowest overpotential of 302 mV at the current density of 10 mA cm−2 and the highest mass activity of 120.5 mA mg−1 at 1.532 V for OER in 0.5 M H2SO4 electrolyte. In addition, a two‐electrode acidic electrolyzer assembled with Ir1Ru3/C at anode and commercial Pt/C at cathode (Pt/C|| Ir1Ru3/C) exhibits a low cell voltage of 1.44 V for achieving the current density of 10 mA cm−2, along with a satisfied 20h durability.
A critical review of life cycle assessment and environmental impact of the well drilling process
Authors: Ghasemi Kamand , Akbari Ali , Jahani Shahriar , Kazemzadeh Yousef 
Published: November 2024

AbstractLife cycle assessment (LCA) is a robust tool for evaluating the environmental impacts of products, processes, and systems throughout their entire life cycle. This article presents a comprehensive investigation into the potential of LCA in drilling methods. The growing emphasis on sustainable and environmentally responsible drilling practices is becoming a critical concern in the drilling industry. As demand for natural resources continues to rise, the need for accurate assessments of the environmental impacts associated with various drilling methods becomes increasingly essential. LCA offers a holistic perspective on all key stages of the drilling industry, providing reliable data and serving as a valuable resource for informed decision‐making aimed at promoting sustainable and optimized drilling techniques. This article delves into the challenges and complexities surrounding LCA evaluations in the context of drilling operations. It underscores the importance of LCA in enhancing the management of drilling cuttings, waste, and surplus materials generated during drilling activities, as well as the effective handling of drilling mud. Additionally, it highlights the critical issue of groundwater contamination resulting from drilling operations. By presenting a holistic view of the life cycle of drilling products and processes, the article offers practical insights into improving and optimizing drilling techniques and waste management strategies. Moreover, the article examines the challenges and potential solutions associated with implementing LCA in these areas. It aims to support responsible and informed decision‐making, ultimately leading to improved drilling performance and enhanced environmental management.