bluuebunny/crossref_metadata_2025_split huggingface dataset:
High helminthic co-infection in tuberculosis patients with undernutritional status in northeastern Ethiopia
Authors: Gashaw Fikru , Bekele Samuel , Mekonnen Yalemtsehay , Medhin Girmay , Ameni Gobena , Erko Berhanu 
Published: October 2019

Abstract

Background
Tuberculosis and parasitosis are the widely distributed diseases in Ethiopia with the leading cause of mortality and morbidity, respectively. There has been no information on the status of co-infections of tuberculosis and parasitosis in Oromia Zone of Amhara Region and South Wollo, Ethiopia. Hence, this study primarily focuses on determining the status of tuberculosis and parasitosis co-infections and associated factors.

Methods
The study was conducted in Oromia Special Zone of the Amhara Regional State and South Wollo Zone, northeastern Ethiopia from April 2015 to January 2017. Tuberculosis cases confirmed by health personnel at the health institutions were the source of the study sample. In a cross-sectional study 384 smear positive pulmonary and extra-pulmonary tuberculosis cases were recruited. Faecal specimens provided by the study participants were examined for parasitic co-infections using direct saline microscopic test, Kato-Katz and concentration techniques. Nutritional status was determined using body mass index and mid-upper arm circumferences. Data were analyzed using descriptive statistical methods and Pearson chi-square.

Results
Tuberculosis and parasitosis co-infection prevalence was 10.8%, and the proportion of intestinal helminths accounted for 9.7% while intestinal protozoa accounted for 1.9%. Cases with single parasitic infection was 89.3% among co-infected individuals. Co-infection of both disease was not significantly associated with gender and age (P > 0.05). The prevalence of undernutrition was 58.6% as determined using body mass index and 73.0% as determined using mid-upper arm circumference with no significant association with gender. Among all forms of tuberculosis cases (384) screened for the study, the bacterial positivity was relatively more common in males (55.5%) than females (44.5%). Tuberculosis lymphadenitis was found to be the most prevalent (85.9%) form of extra-pulmonary tuberculosis with cervical adenopathy (75.3%) being the commonly existing disease.

Conclusions
The rate of helminthic co-infection is predominantly high than that of intestinal protozoa. Single parasitic co-infection was more common than double or multiple co-infections. Both body mass index and mid-upper arm circumference anthropometric parameters revealed greater risk of undernutrition in tuberculosis patients. Thus, screening and prompt treatment of parasites in tuberculosis patients and a support of nutritional supplementation for malnourished tuberculosis patients should be further studied which might enhance the disease treatment and minimize the risk of its complexity.
The prognostic role of IDH mutations in homogeneously treated patients with anaplastic astrocytomas and glioblastomas
Authors: Christians Arne , Adel-Horowski Antonia , Banan Rouzbeh , Lehmann Ulrich , Bartels Stephan , Behling Felix , Barrantes-Freer Alonso , Stadelmann Christine , Rohde Veit , Stockhammer Florian , Hartmann Christian 
Published: October 2019

AbstractThe detection of IDH mutations in patients with diffusely infiltrating malignant astrocytomas resulted in substantial modifications in the concept of WHO classification of these tumors. An important underlying observation was that patients with anaplastic astrocytomas (AA) without IDH mutation had a clinical course similar to that of patients with glioblastomas (GBM). The underlying observations of the German Glioma Network and NOA-04, however, were based on mixed patient cohorts. While most GBM patients received combined radiochemotherapy, patients with AA usually had radiotherapy or chemotherapy only. This intrinsic shortcoming of the study raised the question of whether patients with AA, IDH wildtype, WHO grade III, might have better prognosis if treated with combined radiochemotherapy than patients with GBM receiving the same combination therapy. Thus, the question remains whether the established histopathological grading criteria for malignant astrocytomas in the absence of an IDH mutation are still important if neither vascular proliferation nor necrosis are detectable. All patients in the cohort investigated here with the diagnosis of AA or GBM were subjected to a combined radiochemotherapy according to the Stupp protocol independently of the histopathological diagnosis. Thus, the analysis of these patients allows to clarify whether patients with AA, IDH wildtype, WHO grade III have a prognosis similar to that of GBM, IDH wildtype, WHO grade IV, even under equivalent therapeutic conditions. We determined theIDH1andIDH2status by sequencing, theMGMTstatus by pyrosequencing after bisulfite treatment and theEGFRstatus of the patients by FISH. In fact, the patients with the histopathological diagnosis of an AA IDH wild-type under similar aggressive therapy showed a comparable and therefore no better prognosis (median overall survival (mOS) 16 months) than patients with a GBM (mOS 13 months). Instead, patients with an AA and an IDH mutation receiving the same therapy had a mOS of 54 months. Thus, it can be concluded that in the absence of an IDH mutation, the established histopathological grading criteria ‘necrosis’ and ‘vascular proliferation’ actually lose their prognostic significance. If, on the other hand, patients with malignant astrocytomas and an IDH mutation are examined, there is still a difference between patients with necrosis and/or vascular proliferation and those whose tumors do not show such characteristics. Accordingly, in patients with malignant astrocytomas with IDH mutation it can be concluded that a histological differentiation between AA IDH mutated and GBM IDH mutated remains beneficial from a prognostic perspective.
Mineral deposition and vascular invasion of hydroxyapatite reinforced collagen scaffolds seeded with human adipose-derived stem cells
Authors: Weiss-Bilka Holly E. , Meagher Matthew J. , Gargac Joshua A. , Niebur Glen L. , Roeder Ryan K. , Wagner Diane R. 
Published: October 2019

Abstract

Background
Collagen-based scaffolds reinforced with hydroxyapatite (HA) are an attractive choice for bone tissue engineering because their composition mimics that of bone. We previously reported the development of compression-molded collagen-HA scaffolds that exhibited high porosity, interconnected pores, and mechanical properties that were well-suited for surgical handling and fixation. The objective of this study was to investigate these novel collagen-HA scaffolds in combination with human adipose-derived stem cells (hASCs) as a template for bone formation in a subcutaneous athymic mouse model.

Methods
Collagen-HA scaffolds and collagen-only scaffolds were fabricated as previously described, and a clinically approved bone void filler was used as a control for the material. Constructs were seeded with hASCs and were pre-treated with either control or osteogenic media. A cell-free group was also included. Scaffolds were implanted subcutaneously in the backs of athymic nude mice for 8 weeks. Mineral deposition was quantified via micro-computed tomography. Histological and immunofluorescence images of the explants were used to analyze their vascular invasion, remodeling and cellularity.

Results
Cell-free collagen-HA scaffolds and those that were pre-seeded with osteogenically differentiated hASCs supported mineral deposition and vascular invasion at comparable rates, while cell-seeded constructs treated with the control medium showed lower mineralization after implantation. HA-reinforcement allowed collagen constructs to maintain their shape, provided improved cell-tissue-scaffold integration, and resulted in a more organized tissue when pre-treated in an osteogenic medium. Scaffold type and pre-treatment also determined osteoclast activity and therefore potential remodeling of the constructs.

Conclusions
The results of this study cumulatively indicate that treatment medium and scaffold composition direct mineralization and angiogenic tissue formation in an ectopic model. The data suggest that it may be necessary to match the scaffold with a particular cell type and cell-specific pre-treatment to achieve optimal bone formation.
Sustainable product purchase: does information about product sustainability on social media affect purchase behavior?
Authors: Saeed Muhammad Amad , Farooq Ammara , Kersten Wolfgang , Ben Abdelaziz Semah Ibrahim 
Published: October 2019

Abstract
Nowadays, consumer-to-consumer communication and its impact on purchase decisions have gained substantial consideration owing to digitalization and the emergence of internet-based social media platforms. Social media allows consumers to communicate with thousands of people well beyond their network. Consequently, the demand for transparency from industrial corporations has increased. It even becomes more important to understand how sustainability-related information on social media holds organizations accountable for their wrongdoings and reward them for taking sustainability-related initiatives. In this research work, the authors investigate how information that is available on social media influences consumers’ purchase behavior with regard to sustainable products in the context of developing countries. Therefore, the authors surveyed social media users residing in Pakistan and used partial least squares structural equation modeling (PLS-SEM) to analyze the collected data. The results established that positive and negative sustainability-related information on social media significantly influences consumers’ intention to purchase sustainable products. Furthermore, this study explains that consumers’ willingness to seek sustainability-related information makes them dependent on social media and this dependency on social media affects their intention to purchase.
Patterns of morphological variation in the Schlegel’s Japanese gecko (Gekko japonicus) across populations in China, Japan, and Korea
Authors: Kim Dae-In , Park Il-Kook , Ota Hidetoshi , Fong Jonathan J. , Kim Jong-Sun , Zhang Yong-Pu , Li Shu-Ran , Choi Woo-Jin , Park Daesik 
Published: October 2019

Abstract

Background
Studies of morphological variation within and among populations provide an opportunity to understand local adaptation and potential patterns of gene flow. To study the evolutionary divergence patterns of Schlegel’s Japanese gecko (Gekko japonicus) across its distribution, we analyzed data for 15 morphological characters of 324 individuals across 11 populations (2 in China, 4 in Japan, and 5 in Korea).

Results
Among-population morphological variation was smaller than within-population variation, which was primarily explained by variation in axilla-groin length, number of infralabials, number of scansors on toe IV, and head-related variables such as head height and width. The population discrimination power was 32.4% and in cluster analysis, populations from the three countries tended to intermix in two major groups.

Conclusion
Our results indicate that morphological differentiation among the studied populations is scarce, suggesting short history for some populations after their establishment, frequent migration of individuals among the populations, and/or local morphological differentiation in similar urban habitats. Nevertheless, we detected interesting phenetic patterns that may predict consistent linkage of particular populations that are independent of national borders. Additional sampling across the range and inclusion of genetic data could give further clue for the historical relationship among Chinese, Japanese, and Korean populations of G. japonicus.
How to resolve cryptic species of polypores: an example in Fomes
Authors: Peintner Ursula , Kuhnert-Finkernagel Regina , Wille Viana , Biasioli Franco , Shiryaev Anton , Perini Claudia 
Published: October 2019

AbstractSpecies that cannot be easily distinguished based on morphology, but which form distinct phylogenetic lineages based on molecular markers, are often referred to as cryptic species. They have been proposed in a number of fungal genera, including the basidiomycete genusFomes. The main aim of this work was to test new methods for species delimitation in cryptic lineages of polypores, and to define useful characters for species identification.A detailed examination of a number of differentFomesstrains that had been collected and isolated from different habitats in Italy and Austria confirmed the presence of distinct lineages in theFomes fomentariusclade. Our zero hypothesis was that the Mediterranean strains growing onQuercusrepresent a species which can be delimited based on morphological and physiological characters when they are evaluated in statistically relevant numbers.This hypothesis was tested based on phylogenetic analysis of the rDNA ITS region, morphological characters of basidiomes and pure cultures, growth rates and optimum growth temperature experiments, mycelial confrontation tests, enzyme activity tests and volatile organic compound (VOC) production. The Mediterranean lineage can unambiguously be delimited fromF. fomentarius. A syntype of an obscure and previously synonymized name,Polyporus inzengae, represents the Mediterranean lineage that we recognize asFomes inzengae, a distinct species. The rDNA ITS region is useful for delimitation ofFomesspecies. Moreover, also a variety of morphological characters including hymenophore pore size, basidiospore size, and diameter of skeletal hyphae are useful delimiting characters. The ecology is also very important, because the plant host appears to be a central factor driving speciation. Physiological characters turned also out to be species-specific, e.g. daily mycelial growth rates or the temperature range of pure cultures. The production of VOCs can be considered as a very promising tool for fast and reliable species delimitation in the future.
Case study: Seismic resolution and reservoir characterization of thin sands using multiattribute analysis and bandwidth extension in the Daqing field, China
Authors: Mora David , Castagna John , Meza Ramses , Chen Shumin , Jiang Renqi 
Published: October 2019

The Daqing field, located in the Songliao Basin in northeastern China, is the largest oil field in China. Most production in the Daqing field comes from seismically thin sand bodies with thicknesses between 1 and 15 m. Thus, it is not usually possible to resolve Daqing reservoirs using only conventional seismic data. We have evaluated the effectiveness of seismic multiattribute analysis of bandwidth extended data in resolving and making inferences about these thin layers. Multiattribute analysis uses statistical methods or neural networks to find relationships between well data and seismic attributes to predict some physical property of the earth. This multiattribute analysis was applied separately to conventional seismic data and seismic data that were spectrally broadened using sparse-layer inversion because this inversion method usually increases the vertical resolution of the seismic. Porosity volumes were generated using target porosity logs and conventional seismic attributes, and isofrequency volumes were obtained by spectral decomposition. The resulting resolution, statistical significance, and accuracy in the determination of layer properties were higher for the predictions made using the spectrally broadened volume.
Incidence of Late Relapses in Patients With HER2-Positive Breast Cancer Receiving Adjuvant Trastuzumab: Combined Analysis of NCCTG N9831 (Alliance) and NRG Oncology/NSABP B-31
Authors: Chumsri Saranya , Li Zhuo , Serie Daniel J. , Mashadi-Hossein Afshin , Colon-Otero Gerardo , Song Nan , Pogue-Geile Katherine L. , Gavin Patrick G. , Paik Soonmyung , Moreno-Aspitia Alvaro , Perez Edith A. , Thompson E. Aubrey 
Published: October 2019

PURPOSE Recent trials have shown potential benefit of extended adjuvant endocrine therapy and relatively high risk of recurrence (RoR) after 5 years in hormone receptor-positive (HR+) human epidermal growth factor receptor 2–negative (HER2−) breast cancer. Although risk of late relapse in HR+ HER2− breast cancer is fairly well defined, the risk in HER2-positive (HER2+) breast cancer treated with adjuvant trastuzumab-based chemotherapy remains largely unknown. METHODS We included 3,177 patients with HER2+ breast cancer treated with adjuvant chemotherapy alone or with trastuzumab from the North Central Cancer Treatment Group N9831 (ClinicalTrials.gov identifier: NCT00005970 ) and National Surgical Adjuvant Breast and Bowel Project B-31 (ClinicalTrials.gov identifier: NCT00004067 ) trials. RESULTS Overall, HR+ breast cancer was significantly associated with improved recurrence-free survival (RFS) during the first 5 years (hazard ratio, 0.65; 95% CI, 0.56 to 0.77; P < .001). Among patients treated with trastuzumab, cumulative hazard for RFS was lower in patients with HR+ HER2+ breast cancer during the first 5 years (10.96% v 17.48%; hazard ratio, 0.60; 95% CI, 0.45 to 0.79; P < .001). However, there was no significant difference in RFS based on HR status during years 5 to 10 (hazard ratio, 1.32; 95% CI, 0.93 to 1.88; P = .12). A comparable degree of trastuzumab benefit was observed in HR+ and HR− breast cancers ( P for interaction = .87). Furthermore, we observed low RoR in years 5 to 10 among patients with HR+ HER2+ breast cancer: 3.23% in patients without lymph node involvement (N0) and 6.39% in patients with involvement of one to three lymph nodes (N1). CONCLUSION The benefit of adjuvant trastuzumab persists for a long time. A distinct pattern of recurrence was observed between HR+ and HR− HER2+ disease but with similar degree of benefit from adjuvant trastuzumab. RoR in years 5 to 10 in HR+ HER2+ breast cancer is low, particularly in patients with N0 or N1 disease.
Bone and Soft‐Tissue Sarcoma Risk in Long‐Term Survivors of Hereditary Retinoblastoma Treated With Radiation
Authors: Kleinerman Ruth A. , Schonfeld Sara J. , Sigel Byron S. , Wong-Siegel Jeannette R. , Gilbert Ethel S. , Abramson David H. , Seddon Johanna M. , Tucker Margaret A. , Morton Lindsay M. 
Published: October 2019

PURPOSE Survivors of hereditary retinoblastoma have excellent survival but substantially increased risks of subsequent bone and soft-tissue sarcomas, particularly after radiotherapy. Comprehensive investigation of sarcoma risk patterns would inform clinical surveillance for survivors. PATIENTS AND METHODS In a cohort of 952 irradiated survivors of hereditary retinoblastoma who were originally diagnosed during 1914 to 2006, we quantified sarcoma risk with standardized incidence ratios (SIRs) and cumulative incidence analyses. We conducted analyses separately for bone and soft-tissue sarcomas occurring in the head and neck (in/near the radiotherapy field) versus body and extremities (out of field). RESULTS Of 105 bone and 124 soft-tissue sarcomas, more than one half occurred in the head and neck (bone, 53.3%; soft tissue, 51.6%), one quarter in the body and extremities (bone, 29.5%; soft tissue, 25.0%), and approximately one fifth in unknown/unspecified locations (bone, 17.1%; soft tissue, 23.4%). We noted substantially higher risks compared with the general population for head and neck versus body and extremity tumors for both bone (SIR, 2,213; 95% CI, 1,671 to 2,873 v SIR, 169; 95% CI, 115 to 239) and soft-tissue sarcomas (SIR, 542; 95% CI, 418 to 692 v SIR, 45.7; 95% CI, 31.1 to 64.9). Head and neck bone and soft-tissue sarcomas were diagnosed beginning in early childhood and continued well into adulthood, reaching a 60-year cumulative incidence of 6.8% (95% CI, 5.0% to 8.7%) and 9.3% (95% CI, 7.0% to 11.7%), respectively. In contrast, body and extremity bone sarcoma incidence flattened after adolescence (3.5%; 95% CI, 2.3% to 4.8%), whereas body and extremity soft-tissue sarcoma incidence was rare until age 30, when incidence rose steeply (60-year cumulative incidence, 6.6%; 95% CI, 4.1% to 9.2%), particularly for females (9.4%; 95% CI, 5.1% to 13.8%). CONCLUSION Strikingly elevated bone and soft-tissue sarcoma risks differ by age, location, and sex, highlighting important contributions of both radiotherapy and genetic susceptibility. These data provide guidance for the development of a risk-based screening protocol that focuses on the highest sarcoma risks by age, location, and sex.
Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4
Authors: Velecela Víctor , Torres-Cano Alejo , García-Melero Ana , Ramiro-Pareta Marina , Müller-Sánchez Claudia , Segarra-Mondejar Marc , Chau You-Ying , Campos-Bonilla Begoña , Reina Manuel , Soriano Francesc X. , Hastie Nicholas D. , Martínez Fernando O. , Martínez-Estrada Ofelia M. 
Published: October 2019

ABSTRACT
The epicardium plays a crucial role in embryonic heart development and adult heart repair; however, the molecular events underlying its maturation remain unknown. Wt1, one of the main markers of the embryonic epicardium, is essential for epicardial development and function. Here, we analyse the transcriptomic profile of epicardial-enriched cells at different stages of development and from control and epicardial-specific Wt1 knockout (Wt1KO) mice. Transcriptomic and cell morphology analyses of epicardial cells from epicardial-specific Wt1KO mice revealed a defect in the maturation process of the mutant epicardium, including sustained upregulation of Bmp4 expression and the inability of mutant epicardial cells to transition into a mature squamous phenotype. We identified Bmp4 as a transcriptional target of Wt1, thus providing a molecular basis for the retention of the cuboidal cell shape observed in the Wt1KO epicardium. Accordingly, inhibition of the Bmp4 signalling pathway both ex vivo and in vivo rescued the cuboidal phenotype of the mutant epicardium. Our findings indicate the importance of the cuboidal-to-squamous transition in epicardial maturation, a process regulated by Wt1.
On Optimal Auctions for Mixing Exclusive and Shared Matching in Platforms
Authors: Bhargava Hemant K. , Csapó Gergely , Müller Rudolf 
Published: October 2019

Platforms create value by matching participants on alternate sides of the marketplace. Although many platforms practice one-to-one matching (e.g., Uber), others can conduct and monetize one-to-many simultaneous matches (e.g., lead-marketing platforms). Both formats involve one dimension of private information, a participant’s valuation for exclusive or shared allocation, respectively. This paper studies the problem of designing an auction format for platforms that mix the modes rather than limit to one and, therefore, involve both dimensions of information. We focus on incentive-compatible auctions (i.e., where truthful bidding is optimal) because of ease of participation and implementation. We formulate the problem to find the revenue-maximizing incentive-compatible auction as a mathematical program. Although hard to solve, the mathematical program leads to heuristic auction designs that are simple to implement, provide good revenue, and have speedy performance, all critical in practice. It also enables creation of upper bounds on the (unknown) optimal auction revenue, which are useful benchmarks for our proposed auction designs. By demonstrating a tight gap for our proposed two-dimensional reserve-price-based mechanism, we prove that it has excellent revenue performance and places low information and computational burden on the platform and participants. This paper was accepted by Chris Forman, information systems.