bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Factors Influenced on Oil Rim Breaking up During Gas Cap Development
Authors: Samolovov Dmitriy 
Published: September 2019

Abstract
The main goal of the present paper is to determine main factors affected on oil rim breaking up ratio during gas cap development, to construct analytical model of this process and to develop method for movable oil reserves decrease estimation based on the analytical model.
Field with underlaying oil rim is considered. Gas cap development leads to reservoir pressure decrease, oil viscocity increase due to dissolved gas liberation and underlaying water expands sweeping oil from oil rim and forming immobile oil reserves behind displacement front. For volume of penetrating water estimation material balance equation is considered. Equation is solved in analytical form using linearization of main oil and gas properties deopendencies from pressure. For immobile oil reserves calculation balance equation is considered taking into account initial and residual saturations of oil, gas and water. For determine main factors affected breaking up ratio dimensional analysis is used.
In the present paper main factors affected oil rim breaking up ratio is determined. There are factors characterized reservoir energetic, oil and gas reserves, relative permeability properties, ratio of gas production. During dimensional analysis count of this factors was reduced to five, this is dimensionless potential of gas cap, dimensionless potential of aquifer, dimensionless gas production, displacement efficiency of oil by water and water's redisplacement efficiency of oil sweeping gas. Also it is shown that presence of strong aquifer intensifies breaking up because expanding water forms essential volume of immobile oil reserves.
Novelty of this work consists of analytical model of oil rim breaking up during gas cap development and determination of main factors affected breaking up ratio. Results can be used for estimation of gas cap development on movable oil reserves in oil rim and for estimation of oil rim development efficiency with early gas cap development.
Risk factor for postoperative hemorrhage after cesarean section for placenta previa : A retrospective study
Authors: Ishibashi Hiroki , Miyamoto Morikazu , Soyama Hiroaki , Iwahashi Hideki , Kawauchi Haruka , Takasaki Kazuki , Matuura Hiroko , Nakatsuka Masaya , Hada Taira , Takano Masashi 
Published: September 2019

Abstract
Background
Placenta previa can cause postoperative hemorrhage. Even cases with less intraoperative hemorrhages during cesarean section have the potential risk of developing postoperative hemorrhage. However, there are few reports on the predictive factors of postoperative hemorrhage associated with placenta previa. The aim of this study was to identify the predictive factor for postoperative hemorrhage after cesarean section in women with placenta previa.
Methods
We identified women with placenta previa who underwent cesarean section at our institution between January 2003 and February 2015. All women who received any hemostatic procedure, such as intrauterine balloon tamponade and gauze infiltration during cesarean section were excluded. All women were classified into two groups: Group A, women with massive postoperative hemorrhage, defined as over 500 ml of hemorrhage after cesarean section, and Group B, women without postoperative hemorrhage. A retrospective analysis to identify the predictive factors for postoperative hemorrhage was conducted.
Results
Out of 128 women, 10 (7.8%) were included in Group A and 118 (92.2%) in Group B. There was no statistically significant difference in maternal history between the groups. The number of women suspected to have placental adhesion was higher in Group A than in Group B (p=0.006). Furthermore, the amount of intraoperative hemorrhage in Group A was higher than that in Group B (p=0.025). As treatment for postoperative hemorrhage, more women in Group A received allogenic blood transfusion (p=0.003) and uterine artery embolization (p = 0.010). In univariate analysis, placental adhesion suspected by the surgeon during cesarean section was the predictive factor for postoperative hemorrhage with placenta previa (p=0.002).
Conclusions
When placental adhesion is suspected by surgeons during a cesarean section, additional hemostatic procedures should be performed to prevent possible postoperative hemorrhage.
Increased Th17 and Th22 cell levels predict acute lung injury in patients with sepsis
Authors: Li Gang , Zhang Liangtian , Han Nannan , Zhang Ke , Li Hengjie 
Published: September 2019

Abstract
Background: Acute lung injury (ALI) is one of the major complications of severe sepsis. This study was conducted to investigate the levels of Th22 and Th17 cells in the peripheral blood septic patients with ALI and their clinical significance. Results: A total of 479 septic patients admitted between January 2013 to January 2018 were divided into non-ALI (n = 377) and ALI groups (n = 102) based on the presence or absence of ALI. The levels of Th22 and Th17 cells, interleukin 22 (IL-22), 6 (IL-6) and 17 (IL-17) were determined. Receiver operating characteristic curve (ROC) analysis was performed to assess the early diagnostic value of Th22 and Th17 cells to predict sepsis-induced ALI. The lung injury prediction score (LIPS), IL-6, IL-17, IL-22, and levels of Th17 and Th-22 cells were 9.13, 14.02 ng/L, 13.06 ng/L, 22.90 ng/L, 8.80% and 7.40%, respectively, in the ALI patients and were significantly higher in the ALI group than in non-ALI group (P < 0.05). Pearson correlation analysis showed that LIPS, IL-17, IL-22, Th17 cells and Th22 cells were significant factors affecting sepsis-induced ALI (P < 0.05). The correlation analysis showed that the levels of Th22 cells in the peripheral blood of septic patients with ALI were positively correlated with LIPS, IL-22 and the levels of Th17 cells (P < 0.05), and the levels of Th17 cells were positively correlated with LIPS and IL-17 (P < 0.01). Multivariate logistic regression analysis showed that the LIPS (OR = 1.130), IL-17 (OR = 1.982), IL-22 (OR =2.612) and levels of Th17 (OR = 2.211) and Th22 (OR =3.230) cells were independent risk factor for ALI. The area under the curve of Th22 cells was 0.844 with a cutoff value of 6.81% to predict ALI. The sensitivity and specificity for early diagnosis of sepsis-induced ALI by Th22 cells were 78.72% and 89.13% respectively, which were better but statistically similar as compared with Th17 cells (P > 0.05). Conclusions: The levels of Th22 and Th17 cells in peripheral blood are significantly increased in septic patients with induced ALI, and may be used for early diagnose of sepsis-induced ALI.
GAMYB gene in rye – sequence, polymorphisms, map location, allele-specific markers and relationship with selected agronomic traits
Authors: Bienias Anna , Góralska Magdalena , Masojć Piotr , Milczarski Paweł , Myśków Beata 
Published: September 2019

Abstract
Background. A master GA-induced regulatory protein, crucial for development and germination of cereal grain and involved in anther formation is MYB transcription factor GAMYB, activating a vast number of genes including high-molecular-weight glutenin and α-amylase gene families. This paper presents the first attempt to characterize rye gene encoding GAMYB in relation to its sequence, polymorphisms and phenotypic effects. Results. ScGAMYB gene was identified and mapped on rye chromosome 3R using high-density DArT/DArTseq-based maps developed in two mapping populations. Comparative analysis of the gene sequence revealed its high level of homology to wheat and barley orthologues. Single nucleotide polymorphisms detected among rye inbred lines allowed developing AS-PCR markers for ScGAMYB (ten pairs of primers) which might be used to detect this gene in wide genetic stocks of rye and triticale. Segregation of ScGAMYB alleles showed significant relationship with quantitative traits including plant height, thousand grain weight, α-amylase activity, earliness per se and leaf rolling. Conclusions. The research showed the strong similarity of rye GAMYB sequence to its orthologues in other Graminae and confirmed the position in the genome consistent with the collinearity rule of cereal genomes. The statistically significant, however moderate association of ScGAMYB with many agronomic features has been pointed out, which proved that this gene is a QTL of pleiotropic character. The effect of ScGAMYB on flowering time was statistically the most significant. Developed sequence-based, allele-specific PCR markers could be useful in research and application purposes.
New data on known species of Hirschmanniella and Pratylenchus (Rhabditida, Pratylenchidae) from Iran and South Africa
Authors: Shokoohi Ebrahim , Abolafia Joaquín , Mashela Phatu William , Divsalar Nafiseh 
Published: September 2019

Abstract

Hirschmanniella anchoryzae from Iran and Pratylenchus hippeastri from South Africa were recovered during a survey of plant-parasitic nematodes belonging to the family Pratylenchidae. Both species were studied using morphological and molecular techniques. Hirschmanniella anchoryzae is identified based on the flattened head, short stylet (19–22 µm), excretory pore position (anterior to pharyngo-intestinal junction), spicule length (27–30 µm), and existence of an axial mucro at the tail end. Phylogenetic analysis using 28S rDNA showed monophyly of Hirschmanniella which Iranian H. anchoryzae placed close to H. halophila (EU620464; EU620465). This result was supported by the principal component analysis of Hirschmanniella species. SEM observation of the South African population of P. hippeastri showed the presence of two annuli in the lip region. Morphometric characters resembled those of specimens earlier reported from South Africa. Hierarchal cluster using morphometrical criteria showed that the Floridian (USA) and South African populations form a group. However, the principal component analysis showed variation within this species. The molecular study of P. hippeastri populations using 18S, ITS, 28S rDNA, and COI of mtDNA showed that all P. hippeastri cluster in one group and confirmed the identification of the species using both morphological and molecular techniques. In addition, the results indicated that South African populations group close to the USA populations. Illustrations of both species including light and scanning electron microscopy observations for P. hippeastri are provided.
Developing a real-time PCR diagnostic method for a potential threat to chrysanthemum, Paratylenchus dianthus
Authors: Kawanobe Masanori , Toyota Koki , Uchihara Hidehito , Takae Mikoto 
Published: September 2019

Abstract
Chrysanthemum is a very popular flower in Japan and is known to be infected by many soil-borne plant pathogens including nematodes. A nematode survey in six chrysanthemum fields in Okinawa, Japan, found Pratylenchus, Meloidogyne, and Paratylenchus (P. dianthus). The first two genera are known as plant pathogens against chrysanthemum, however, Paratylenchus dianthus has not been reported previously. Chrysanthemum seedlings were grown in pots containing soil infected only with P. dianthus for two months in 2017 and 2018. The nematicide imicyafos was applied in triplicates to half of the pots (treated) while the other half were left without the nematicide (non-treated). Plant height and dry plant weight of the imicyafos treated plants exceeded those of the control plants. Also, single-photon avalanche diode value of chrysanthemum leaves was higher in imicyafos treated plants than in the non-treated plants at two-month after planting. The results suggest that P. dianthus may suppress the growth of chrysanthemum. For high-throughput nematode diagnosis, a real-time PCR primer set specific to P. dianthus was developed and its sensitivity to quantify P. dianthus was confirmed with a proper calibration curve. The calibration curve was developed in a simplified approach by using serially diluted DNA extracted from individual nematodes.
Movement of seed- and soil-applied fluopyram in soil columns
Authors: Faske Travis R. , Brown Katherine 
Published: September 2019

Abstract
The movement of seed- and soil-applied fluopyram was evaluated in soil columns. The nematicide was sampled at three soil depths and used in a nematode motility bioassay. Based on Meloidogyne incognita mortality, the downward movement of soil-applied fluopyram was affected by soil type and application method. No nematode-toxic levels of soil-applied fluopyram were detected past 5 cm depth in sandy loam soil compared to 10 cm depth in sandy soil. A slower rate of water infiltration had little impact on the movement of soil-applied fluopyram in sandy soil, but did affect the movement of soil-applied abamectin. In the seed-applied nematicide experiments, a greater effect on nematode mortality was observed at the 0 to 5 cm depth in sandy soil with fluopyram- than abamectin-treated cotton seed, whereas a similar effect was observed with soybean seed. No effect on nematode motility was observed with other seed-applied nematicides, thiodicarb, and Bacillus firmus. Overall, soil-applied fluopyram had a greater effect on M. incognita mortality at 10 cm depth in sandy soil than seed-applied fluopyram. These data provide a better understanding as to the movement of fluopyram as affected by soil type, water infiltration rates, and application methods.
Morphological and Molecular Characterization of Oscheius saproxylicus sp. n. (Rhabditida, Rhabditidae) From Decaying Wood in Spain, With New Insights into the Phylogeny of the Genus and a Revision of its Taxonomy
Authors: Abolafia Joaquín , Peña-Santiago Reyes 
Published: September 2019

Abstract
A new species of the genus Oscheius, O. saproxylicus sp. n., collected in decaying wood obtained from an orchard in Southern Iberian Peninsula, is reported. A detailed description, including morphometrics, LM and SEM images, and molecular (18S and 28S rDNA genes) information is provided. The female is characterized by a moderately long body, lateral field with three longitudinal ridges, midbody vulva, and conical tail with acute tip. It was distinguished from its closest relative, O. dolichura, by a shorter tail and longer rectum. The male was not found. Morphological and molecular data support its belonging to Dolichura-group. Molecular analyses show that both Insectivorus and Dolichura groups are related to each other, being proposed as subgenera of the genus Oscheius as Oscheius and Dolichorhabditis. Finally, an updated taxonomy of the genus is presented, with generic and subgeneric diagnoses, list of species and a key to their identification.
Hatching and Mortality of Meloidogyne enterolobii Under the Interference of Entomopathogenic Nematodes In vitro
Authors: Damascena Alixelhe Pacheco , Ferreira Júlio César Antunes , Costa Marylia Gabriella Silva , de Araujo Junior Luis Moreira , Wilcken Silvia Renata Siciliano 
Published: September 2019

Abstract
Plant parasitic nematodes have become one of the main problems in the tomato cultivation. Among these, Meloidogyne enterolobii presents great challenges to the farmer, since it is a polyphagous species and difficult to control. The entomopathogenic nematodes (EPNs) present as potential for biological control of this pathogen. The objective of the study was to evaluate the interference of EPNs S. brazilense, S. feltiae, S. rarum, H. amazonensis and H. bacteriophora on hatching and mortality of M. enterolobii. 500 eggs of this nematode and 1.000 infective juveniles of each EPN species were placed in a plastic pot totaling 25 mL of suspension and kept in an incubator at 25°C. The number of juveniles hatched in the suspension was counted every 2 days, until 10 days. After 10 days of evaluations, the remaining suspension (15 mL) containing M. enterolobii and EPNs was inoculated into Rutgers tomato seedlings. The suspension contained approximately in 300 eggs of M. enterolobii occasional juveniles and 600 IJ of each nematode species. Sixty days after inoculation were evaluated gall indexes, egg mass indexes, total number of eggs and juveniles of M. enterolobii and reproductive factor was calculated. In the mortality experiment, 500 infective juveniles of M. enterolobii and 1.000 juveniles of each EPN species were placed in a plastic pot totaling 25 mL of suspension. The evaluation of juvenile mortality was performed by counting of the mobile and immotile nematodes, by adding two drops of NaOH to the nematode suspension. It was verified that on the 10th day all ENPs provided reduction in the hatching of M. enterolobii. In the pot experiment it was found thato gall index, egg mass indexm, nematodes total number and reproduction factor were significantly reduced in treatments with all species of EPNs tested. However, in the mortality test, only EPNs S. brazilense and S. rarum provided mortality on the second day and H. bacteriophora affected mortality on the 4th day. In the other evaluations, there was no statistical difference. The results highlight the potential of the use of EPNs in programs of integrated management of M. enterolobii in tomato.
Morphological and Molecular Characterization of Coslenchus paramaritus n. sp. and C. cancellatus (Cobb, 1925) Siddiqi, 1978 (Nematoda: Tylenchidae) from Iran
Authors: Hosseinvand Manouchehr , Eskandari Ali , Ghaderi Reza 
Published: September 2019

Abstract

Coslenchus paramaritus n. sp. and C. cancellatus were identified based on morphological, morphometric, and molecular characteristics. The new species, C. paramaritus n. sp., is characterized by its cuticle with 18 longitudinal ridges excluding lateral lines, wide cuticular annuli (2.6–3.0 µm), a long tail (94–128 µm), the presence of males and the absence of sexual dimorphism in head shape. Molecular phylogenetic studies of the new species using D2–D3 expansion segments of 28S rRNA revealed that the new species formed a sister clade with three unknown populations of Coslenchus in Bayesian inference (BI) phylogeny, while C. cancellatus formed a sister clade with other Coslenchus species including C. oligogyrus, C. franklinae, C. costatus, C. turkeyensis and three unknown populations. C. cancellatus is recovered from Iran for the first time.
Validation of the Chemotaxis of Plant Parasitic Nematodes Toward Host Root Exudates
Authors: Liu Wenshan , Jones Alexis L. , Gosse Heather N. , Lawrence Kathy S. , Park Sang-Wook 
Published: September 2019

Abstract
Plant parasitic nematodes (PPN) are microscopic soil herbivores that cause damage to many economic crops. For the last century, it has been proposed that chemotaxis is the primary means by which PPN locate host plant roots. The identities and modes of action of chemoattractants that deliver host-specific messages to PPN, however, are still elusive. In this study, a unique multidimensional agar-based motility assay was developed to assess the impacts of root exudates on the short-range motility and orientation of PPN. Three PPN (Rotylenchulus reniformis, Meloidogyne incognita and Heterodera glycines) and root exudates from their respective host and non-host plants (cotton, soybean, and peanut) were used to validate the assay. As predicted, R. reniformis and M. incognita were attracted to root exudates of cotton and soybean (hosts), but not to the exudates of peanut (non-host). Likewise, H. glycines was attracted to soybean (host) root exudates. These results underpinned the intrinsic roles of root exudates in conveying the host specificity of PPN. In particular, PPN selectively identified and targeted to hydrophilic, but not hydrophobic, fractions of root exudates, indicating that groundwater should be an effective matrix for chemotaxis associated with PPN and their host plant interactions.
Effects of Somatostatin and Vitamin C on the Fatty Acid Profile of Breast Cancer Cell Membranes
Authors: Hanikoglu Aysegul , Kucuksayan Ertan , Hanikoglu Ferhat , Ozben Tomris , Menounou Georgia , Sansone Anna , Chatgilialoglu Chrys , Di Bella Giuseppe , Ferreri Carla 
Published: September 2019

Background:Vitamin C (Vit C) is an important physiological antioxidant with growing applications in cancer. Somatostatin (SST) is a natural peptide with growth inhibitory effect in several mammary cancer models.Objective:The combined effects of SST and Vit C supplementation have never been studied in breast cancer cells so far.Methods:We used MCF-7 and MDA-MB231 breast cancer cells incubated with SST for 24h, in the absence and presence of Vit C, at their EC50 concentrations, to evaluate membrane fatty acid-profiles together with the follow-up of EGFR and MAPK signaling pathways.Results:The two cell lines gave different membrane reorganization: in MCF-7 cells, decrease of omega-6 linoleic acid and increase of omega-3 fatty acids (Fas) occurred after SST and SST+Vit C incubations, the latter also showing significant increases in MUFA, docosapentaenoic acid and mono-trans arachidonic acid levels. In MDA-MB231 cells, SST+Vit C incubation induced significant membrane remodeling with an increase of stearic acid and mono-trans-linoleic acid isomer, diminution of omega-6 linoleic, arachidonic acid and omega-3 (docosapentaenoic and docosadienoic acids). Distinct signaling pathways in these cell lines were studies: in MCF-7 cells, incubations with SST and Vit C, alone or in combination significantly decreased EGFR and MAPK signaling, whereas in MDA-MB231 cells, SST and Vit C incubations, alone or combined, decreased p-P44/42 MAPK levels, and increased EGFR levels.Conclusion:Our results showed that SST and Vit C can be combined to induce membrane fatty acid changes, including lipid isomerization through a specific free radical-driven process, influencing signaling pathways.
The Correlation between EGFR and Androgen Receptor Pathways: A Novel Potential Prognostic Marker in Gastric Cancer
Authors: Fard Shahrzad S. , Saliminejad Kioomars , Sotoudeh Masoud , Soleimanifard Niloofar , Kouchaki Shaghayegh , Yazdanbod Mansour , Mahmoodzadeh Habibollah , Ghavamzadeh Ardeshir , Malekzadeh Reza , Chahardouli Bahram , Alimoghaddam Kamran , Ghaffari Seyed H. 
Published: September 2019

Background:
Despite worthy biologic rationale and numerous studies introducing therapeutic
strategies targeting Epidermal Growth Factor Receptor (EGFR), phase III clinical trials have claimed that these
current anti-EGFR agents did not significantly improve overall survival of Gastric Cancer (GC) patients. Therefore,
to discover flawless candidates of anti-EGFR therapy and ideal prognostic markers, innovative studies are
warranted.

Methods:
The aim of this study was to assess the expression profile of EGFR in GC, adjacent non-tumor and
normal gastric tissues by qRT-PCR, investigating the association of EGFR expression with clinicopathological
features, evaluating possible molecular interaction between EGFR and Androgen Receptor (AR), and elucidating
novel prognostic marker using Cox regression model.

Results:
Among 60 GC patients, 70% (42/60) overexpressed EGFR relative to normal gastric tissues. EGFR
overexpression was significantly correlated with the AR overexpression in GC patients. Although EGFR overexpression
was remarkably associated with unfavorable outcomes (HR= 4.067, 95% CI= 1.228-13.467, p= 0.022),
it was not an independent prognostic factor adjusted for other variables. However, we provided evidences that
simultaneous evaluation of EGFR and AR expression, could independently predict the outcome of GC patients
and could use as a precise prognostic marker. Moreover, it was revealed that induction or inhibition of AR
signaling could alter the mRNA expression of EGFR in GC cell lines.

Conclusion:
By targeting AR and EGFR using a potent AR inhibitor such as Enzalutamide, we postulate the
possible crosstalk between EGFR and AR pathways in GC. Moreover, our study provided evidences elucidating
a novel promising marker, simultaneous evaluation of EGFR and AR expression, which could properly predict
prognosis of gastric cancer patients.