bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiomyopathies associated with the DES gene mutations: molecular pathogenesis and gene therapy approaches
Authors: Kochergin-Nikitsky K. S. , Zaklyazminskaya E. V. , Lavrov A. V. , Smirnikhina S. A. 
Published: July 2019

Cardiomyopathy (CMP) is a common group of cardiovascular disorders. Genetic (primary) cardiomyopathies are related to abnormalities in more than 100 genes, including the DES gene encoding desmin protein. Desmin is an essential member of the intermediate filaments, ensuring the structural and functional integrity of myocytes. Mutations in the DES gene result in desmin-related cardiomyopathy with progressive course and poor prognosis. By now, specific therapy for cardiomyopathy has not been developed. Existing conservative and surgical treatment modalities target the rate of heart failure progression and sudden cardiac death prevention but have limited efficacy. The development of gene therapy and genome editing could allow for creating effective and specific methods of gene-based therapy for desminopathies. A number of studies have been published on the use of gene therapy for various genetic cardiomyopathies including those caused by the DES gene mutations, while genome editing has not been used yet. However, promising results have been obtained with CRISPR/Cas9 and TALEN editing systems to correct for “gain-of-function mutations” in some other genes, such as MYBPC3 and PLN. There is also evidence of the possibility to reduce the symptoms of desmin-related cardiomyopathy up to the normal function by knocking out the mutant DES allele, and preserved protein function provided by expression of the normal allele. We believe that genome editing approaches have an open perspective into the development of specific and effective methods to treat desminopathies.
Parésia Hipocaliemica em Adolescente: Um Caso Clínico
Authors: Carvalho Ana Catarina , Matos Joana , Lira Susana , Reis Ana 
Published: July 2019

A paralisia periódica hipocaliémica familiar é uma doença neuromuscular autossómica dominante, rara, caracterizada por crises episódicas de paralisia flácida acompanhadas de hipocaliemia. Apresenta-se o caso de um adolescente do sexo masculino, com 15 anos de idade, observado no Serviço de Urgência de Pediatria por parésia flácida dos quatro membros de início subito e agravamento progressivo. Na anamnese verificou-se a ingestão de corticoesteroide e antihistaminico no dia anterior por urticária e história familiar de episódios transitórios de paralisia flácida na adolescência, assintomáticos após a 4ª década de vida, sem diagnóstico estabelecido. Os exames auxiliares de diagnóstico revelaram hipocaliemia (K+ < 2,4 mEq/L), sem hipocaliúria e sem outras alterações. Verificou-seresolução da sintomatologia após suplementação com potássio até normalização da caliemia. A paralisia flácida é uma forma rara de apresentação da hipocaliemia. Diversas etiologias podem estar envolvidas na criança ou adolescente que se apresenta com com paralisia flácida de início agudo. Com a descrição deste caso alerta-se para a importância do conhecimento desta entidade, porque se reconhecidos e tratados apropriadamente, os doentes geralmente recuperam sem sequelas.
O Ensino da Dor Crónica em Portugal: As Perspectivas dos Estudantes de Medicina e dos Internos do Ano Comum
Authors: Cristóvão Irina , Reis-Pina Paulo 
Published: July 2019

Introdução: A Associação Internacional para o Estudo da Dor defende um curriculum mandatório sobre dor crónica nos cursos de Medicina. Foi objectivo deste estudo conhecer a opinião dos estudantes finalistas de Medicina e dos internos do ano comum sobre o ensino da dor crónica nas oito escolas médicas Portuguesas.Material e Métodos: Estudo quantitativo. Questionário electrónico (30 questões), com respostas voluntárias e anónimas; disponível no primeiro trimestre 2016.Resultados: Houve 251 respostas provenientes de 142 finalistas e 109 internos (mulheres 72,9%; idade 25,3 ± 1,6 anos). A dor é um sinal vital (92,4%); mas 18,7% apenas a avaliava caso o doente se queixasse. As escalas de auto-avaliação da dor eram conhecidas (87,2%) e as de hétero-avaliação não (70,9%). A dor não era avaliada porque o doente não manifestava dor; falta de tempo; consultas de duração curta. A formação foi insuficiente sobre opioides (78,1%), fisiopatologia e tratamento da dor (66,1%) e como entrevistar o doente com dor (67,7%); durou 1 a 10 horas (49,8%). A depressão respiratória por opioides é preocupante (56,2%). O risco de usar opioides é superior ao benefício clínico (33,5%).Discussão: O ensino da dor crónica é disperso, pouco estruturado e opcional. Para 98,4% da amostra é relevante haver mais educação sobre dor crónica. Esta deve ocorrer no quinto ano do curso médico, com mais de 15 horas. São aconselhados estágios em consultas de dor crónica.Conclusão: São necessárias mudanças nos curricula pré-graduados para que os futuros médicos desenvolvam competências e combatam o sofrimento ‘evitável’ dos seus doentes.
Global Impact of Peripheral Obstructive Arterial Disease in Portugal: An Eight Year Study
Authors: Moutinho Mariana , Simões Inês , Rodrigues Sónia , Abreu Daisy , Silva Emanuel , Sousa Paulo , Fernandes José Fernandes e 
Published: July 2019

Introduction: Peripheral arterial disease has an important impact on morbidity/mortality. The objective of this study was to quantify the impact of this disease in Portugal during the last eight years, expressed by the volume of admissions, treatment strategies and associated morbidity and mortality.Material and Methods: We collected data from the Diagnosis Related Group national database on primary diagnosis, procedures codes, demographic variables, a number of risk factors, and mortality of all cases admitted from 2009 to 2016 with a primary diagnosis of peripheral arterial disease coded according to the 9th revision of the International Statistical Classification of Diseases and Related Health Problems (ICD-9).Results: In this study, peripheral arterial disease led to 27 684 hospitalisations, which corresponded to 26.7% of all admissions for vascular disease in this period. Approximately 49.9% of patients were admitted to the emergency department. The volume of procedures in patients with claudication decreased over the eight years, unlike patients with critical ischaemia, in which the number of procedures increased.Discussion: Age and the presence of cardiovascular risk factors have been associated with the severity of disease, as observed in our series. Overall hospital mortality varied, being significantly higher in patients with more advanced severity of the disease.Conclusion: Peripheral arterial disease represents an important burden in the overall volume of admissions in Portuguese public hospitals. A large number of patients was admitted in the context of emergency.
Peningkatan Kemandirian Anak Usia 4-5 Tahun Melalui Bercerita
Authors: Pareira Mariana Ikun RD , Atal Naomi Habi 
Published: July 2019

Autonomy is important to be taught early because makes it will easier for children to carry out further education. Autonomy also leads children to have high self-esteem and intrinsic motivation. The most prominent problem in children at TK Tunas Harapan Emaus Liliba Kupang is autonomy. Autonomy can be promoted in various ways, one of them which is story telling. The purpose of this study is to find out whether there is an increase in autonomy of children ages 4-5 years after story telling. The research method used in this research is experimental method with quantitative approach. The research design used is a non-equivalent research design or also called "one group pre test and post test design" which is a research design used by giving before and after the activitiies test in a single group research sample. The results of this study indicate that by the Wilcoxon test showed a significant increase in autonomy of children ages 4-5 years (p&lt;0.05)
Early plant defense-related responses triggered by a novel glycoprotein from Streptomycin
Authors: Han Lirong , Sun Yubo , Zhou Xin , Hao xinchang , Wu Meng , Zhang Xing , Feng Juntao 
Published: July 2019

Abstract
Background

A novel glycoprotein from Streptomyces sp. ZX01, GP-1, has high activity against tobacco mosaic virus (TMV). With small molecular weight and water-soluble characteristics, GP-1 has tremendous commercial value. Though previous study indicated a plant immunity-inducing effect, the antiviral mechanism of GP-1 is still unclear.

Results

In this study, early plant defense-related responses, such as Ca2+ transient peak, callose apposition, oxidative burst, hypersensitive response (HR), programmed cell death (PCD), NO rising and stomatal closure, were thoroughly investigated and we studied the mechanism of how GP-1 can induce virus resistance in Nicotiana benthamiana in additional detail. Results showed that GP-1 could induce [Ca2+]cyt rapidly both in tobacco leaves and suspension cells, followed by ROS and NO elevation. Similar with typical pathogen-associated molecular patterns (PAMPs), GP-1 induces callose deposition and stomatal closure to form defense barriers for pathogen invasion. The expression of defense-related genes further supported these phenomena. The gene with CAM5 was obtained by RNA sequencing in Nicotiana benthamiana.

Conclusions

Through analyses of expression of SA, JA and CAM5, it was shown that GP-1 enhanced the virus resistance of tobacco by improving expression of CAM5 and the SA and JA content.
Perception of Implicit Biases within Internal Medicine Residency Recruitment
Authors: Heath Janae Kathleen , Barton Todd D. , Rogers Marisa A. , Dine C. Jessica 
Published: July 2019

Abstract
Background: Few studies have addressed biases within residency admissions. Understanding the perceptions of implicit biases (IB) within residency admissions is essential to identify targets for potential interventions. Our objectives were to assess the interviewers’ perception of IB within the internal medicine (IM) residency interview process and the impact of former Implicit Association Test (IAT) completion and/or IB training on these perceptions.

Methods: We surveyed all interviewers within an IM Residency program following the 2017-2018 interview cycle, assessing the perception of global and individual IB on evaluation of candidates, the perceived role of IAT completion and IB training, and mitigation strategies employed. Responses were assessed in four-subgroups based on exposure to IAT or IB training, analyzed with one-way ANOVA testing. Thematic analysis was performed of the free-text comments.

Results: Of 113 interviewers, 68 (60%) completed the survey. The majority (79%, n=54) agreed or strongly agreed that biases exist within interviews, while 59% (n=40) agreed or strongly agreed that their own biases impact interview evaluations. The belief of one’s own biases impacting evaluation differed significantly between subgroups (52% in those without any IAT/training, compared to 69% with both, p .05). Additionally, 90% (n=26) of those that had completed both IAT or IB training described using strategies to mitigate bias in the interview process, compared to 52% (n=11) that had not completed either (p < 0.001). Qualitative analysis revealed differing mitigation strategies between subgroups.

Conclusions: Self-reported bias in the residency interview process exists. IB training and IAT were associated with self-reported implementation of mitigation strategies.
Conjoined versus separate technique: a meta-analysis of anastomosis techniques for urinary diversion after bladder cancer
Authors: Daqian Liu , Yonghua Wang , Yang Zhao , Xuecheng Yang , xinsheng wang , Haitao Niu 
Published: July 2019

Abstract
Introduction: This was a meta-analysis of studies on conjoined and separate anastomosis for urinary diversion after radical cystectomy due to bladder cancer.
Methods: We searched databases (PubMed, Embase and the Cochrane Central Register of Controlled Trials) for studies regarding anastomosis for urinary diversion due to bladder cancer. The meta-analysis was designed to determine the difference between conjoined and separate anastomosis. Six studies including 1601 patients were considered in the meta-analysis (654 in the separate group and 947 in the conjoined group).
Results: There were no significant differences with respect to age, gender or duration of follow-up. There were seven studies that compared the rate of stricture and the rate of hydronephrosis. For overall stricture, there were 186 of 1163 ureters in the separate group and 64 of 1718 ureters in the conjoined group (odds ratio, 4.53; 95% CI, 2.01-10.22; P = 0.0003). For overall hydronephrosis patients, there were 55 of 205 ureters in the separate group and 51 of 254 ureters in the conjoined group (odds ratio, 1.48; 95% CI, 0.95-2.29; P = 0.08).
Conclusion: The separate anastomosis had a higher ratio of strictures than did the conjoined group. The separate anastomosis group experienced hydronephrosis more often than did the conjoined anastomosis group. The separate anastomosis is the better choice for surgery during the operation.
MAINTAINING IMMOBILISATION DEVICES ON TRAUMA PATIENTS DURING CHEST AND PELVIC X-RAY: A FEASIBILITY STUDY
Authors: Stokkeland Pål Johan 
Published: July 2019

Abstract
BACKGROUND

The aim of our study was to investigate the effect of different vacuum mattresses on standard resuscitation bay conventional radiography of chest and pelvis, especially regarding artefacts.

METHODS

We used a mobile X-ray machine to perform chest and pelvic conventional radiography on an anthropomorphic whole body phantom with a trauma transfer board, three different vacuum mattresses and without any stabilization device. The vacuum mattresses were investigated in activated, deactivated and stretched after deactivated states. Two radiologists assessed the artefacts independently. Agreement was measured using kappa coefficient.

RESULTS

All radiographs were of good technical quality and fully diagnostic.

With the exception of one disagreed occurrence, artefacts were seen to hamper clinical judgement exclusively with activated vacuum mattresses.

There was substantial agreement between the two radiologists on artefact assessment. The observed agreement was 0,82 with a kappa coefficient of 0,71. The first vacuum mattress caused no artefacts hampering with clinical judgment.

CONCLUSION

Our study concludes that it is feasible to maintain some vacuum mattresses through resuscitation bay conventional radiography of chest and pelvis. They do not result in artefacts hampering with clinical judgment. Our vacuum mattress nr 1 is recommendable for this purpose. Together with our previous findings our present results indicate that some vacuum mattresses may be used throughout the initial resuscitation bay assessment and CT examination.
PENGARUH INFUSA TEH HITAM (CAMELIA SINENSIS) TERHADAP GAMBARAN HISTOPATOLOGI HEPAR, RENAL DAN JUMLAH SEL-SEL ALFA DAN BETA PANKREAS TIKUS JANTAN SPRAGUEDAWLEY DIINDUKSI ETANOL 20%
Authors: Rosalia Ayu Anita , Indrasari Maria Chrisna , Tangsilan Maria Anasthasia , Jayadi Tejo , Danu Sulanto Saleh 
Published: July 2019

Latar Belakang: Fungsi antioksidan dari teh telah banyak diteliti. Teh
hitam memiliki kandungan utama theaflavins, thearubigins dan catechins.
Konsumsi etanol kronik dalam jumlah besar dapat menyebabkan gangguan fungsi
hepar, pankreas dan ren, yang disebabkan terutama disebabkan oleh proses stres
oksidatif. Tujuan penelitian ini adalah untuk mengkaji pengaruh infusa teh hitam
terhadap gangguan beberapa organ tersebut diinduksi etanol diteliti.
Metode: Penelitian ini merupakan penelitian eksperimental murni
dikerjakan dengan rancangan completely randomized design, dengan 6 kelompok
eksperimental yang masing-masing menggunakan 5 ekor tikus. K1 kontrol
normal; K2 diberi infusa teh hitam 0,252 gr/ 200 grBB; (K3) diberi etanol 20%
dengan volume 2 ml/ hari; K4, K5 dan K6 yaitu kelompok perlakuan diberi etanol
20% volume 2 ml, 1 jam kemudian diberi infusa teh hitam dengan dosis 0,126 gr/
200 grBB; 0,252 gr/ 200 grBB;0, 50 gr/ 200 grBB setiap hari. Pada hari ke 31
hewan coba diterminasi dan diambil jaringan hepar, pankreas, dan renal.
Kemudian dilakukan pembuatan sediaan histopatologi pada organ hepar dan
ginjal, sediaan dengan pewarnaan histokimia Victoria blue.
Hasil: Pengamatan dan analisis sediaan histopatologi hepar menunjukkan
adanya perbedaan bermakna p=0,004 (p<0,05);sediaan pankreas menunjukkan
adanya perbedaan bermakna jumlah rata-rata sel alfa dan sel beta pulau
Langerhans pankreas, yaitu p=0,002 untuk sel alfa dan 0,00 untuk sel beta
(p<0,05); sediaan renal menunjukkan adanya perbedaan bermakna rata skor
kerusakan ginjal p=0,001 (p<0,05).
Kesimpulan: Infusa teh hitam memperbaiki gambaran histopatologi hepar
dan ren, serta meningkatkan jumlah sel-sel alfa dan sel-sel beta pankreas pada
tikus putih (Sprague Dawley) yang diberi etanol 20% kronik.
PATOFISIOLOGI STROKE ISKEMIA PADA ANAK DENGAN SICKLE CELL DISEASE
Authors: Harisa Nella Harisa , . Paryono , Dahlan Pernodjo 
Published: July 2019

Stroke pada anak relatif lebih jarang dijumpai bila dibanding dengan pada
orang dewasa. Meskipun mekanisme dari stroke iskemia pada anak dan dewasa
adalah sama namun perbedaan yang paling mendasar dari keduanya adalah
bahwa faktor risiko stroke pada anak sangat beragam. Pada pasien dewasa, faktor
risiko vaskular (hipertensi, diabetes, merokok, dislipidemia) yang dikenal dengan
faktor klasik dapat ditemukan secara jelas. Tidak demikian dengan penyebab
stroke pada anak, di mana dilaporkan yang paling sering adalah penyakit jantung
kongenital serta kelainan hematologi.
Sickle Cell Disease (SCD) adalah salah satu penyebab paling umum dari
stroke pada anak, di mana anak dengan SCD berpotensi 300 kali lebih tinggi
daripada anak tanpa SCD. Angka rekurensi cukup tinggi dan dapat mencapai
20%, bahkan pada kasus dengan faktor risiko multipel, angka rekurensi mencapai
42%.
Hemoglobin S sebagai dasar patologis pada SCD ternyata bukan hanya
menyebabkan perubahan membran eritrosit sickle menjadi kaku sehingga
membuat sel darah ini sulit melewati pembuluh-pembuluh darah terutama yang
kecil dan mengakibatkan vasooklusi. Namun juga menjadikan eritrosit menjadi
lebih mudah pecah (hemolisis) dan memperparah kondisi dengan adanya deplesi
nitric oxide (NO) dan arginine sebagai akibat lepasnya sel plasma bebas
intravaskular. Rangkaian proses kompleks tersebut diikuti dengan injuri endotel
akibat hipoksia, peningkatan stres oksidatif, adhesi abnormal dari sickle eritrosit
ke endotel, dan peradangan yang disebabkan oleh kondisi reperfusion injury.
PUBLIKASI PEMBERITAAN DALAM PEMBENTUKAN CITRA LEMBAGA
Authors: Mukaromah Mukaromah , Yusriana Amida 
Published: July 2019

<p>Mass media is one of the media publications to the public in the formation of the Image. Important positive images are formed to foster public trust. News in the newspaper media is still interesting to be appointed in an effort to build a positive image of educational institutions. In addition to targeting the masses of young people, higher education institutions also target parents who are still familiar with conventional media such as newspapers. The case study in this study was Dian Nuswantoro University (Udinus) and news in the Suara Merdeka newspaper. Suara Merdeka is interesting to be appointed because the local central Java newspaper has amounts of printing and is still an alternative reading in the Central Java region where this segmentation is in line with Udinus segmentation which is domiciled in Semarang, Central Java.<br />This study aims to analyze the theme of news that often arises about the institution, the frequency of occurrence of coverage in certain periodizations. Furthermore, this can be observed how the reporting of a media that has the power to shape the image of the institution. The method used is quantitative content analysis, which describes how the contents of the media using thematic and physical recording units are analyzed using the concept of public relations about media and the formation of the image of the institution.<br /> <br />The result can be seen that there are 9 sections in Suara Merdeka which present Udinus related news, namely Semarang Metro rubric, Edukasia, Screen, Page 1, Business Economy, Around Young Tugu, Salatiga News, Youth Voice Expressions. Screen Rubric occupies the highest position in the news, which is 70.3%. For the<br />theme that is often reported, there are four types of reporting, namely news related to the world of academics (products), related to environmental responsibility (CSR), reporting related the environment and reporting related<br />to the activities of communicating the activities of the institution.</p><p> </p>
Graphene and Graphene-Based Materials in Biomedical Applications
Authors: Ansari Mohammad Omaish , Gauthaman Kalamegam , Essa Abdurahman , Bencherif Sidi A. , Memic Adnan 
Published: July 2019

:
Nanobiotechnology has huge potential in the field of regenerative medicine. One of the main
drivers has been the development of novel nanomaterials. One developing class of materials is graphene
and its derivatives recognized for their novel properties present on the nanoscale. In particular,
graphene and graphene-based nanomaterials have been shown to have excellent electrical, mechanical,
optical and thermal properties. Due to these unique properties coupled with the ability to tune their
biocompatibility, these nanomaterials have been propelled for various applications. Most recently, these
two-dimensional nanomaterials have been widely recognized for their utility in biomedical research. In
this review, a brief overview of the strategies to synthesize graphene and its derivatives are discussed.
Next, the biocompatibility profile of these nanomaterials as a precursor to their biomedical application
is reviewed. Finally, recent applications of graphene-based nanomaterials in various biomedical fields
including tissue engineering, drug and gene delivery, biosensing and bioimaging as well as other biorelated
studies are highlighted.
Molecular Docking Supplements an In vitro Determination of the Leading CYP Isoform for Arylpiperazine Derivatives
Authors: Ulenberg Szymon , Bączek Tomasz , Zieliñska Joanna , Belka Mariusz , Król Marek , Herold Franciszek 
Published: July 2019

Background:Molecular docking has often been used before to calculate in silico affinity of drugs towards their molecular target, but not to estimate leading CYP isoform responsible for metabolism of studied compounds.Objective:The aim of this study is to present molecular docking as a valid alternative for costly in vitro studies resulting in estimation of leading CYP isoform.Method:In vitro part was based on incubations of studied compounds with isolated CYP3A4 isoform followed by LC-MS analysis. The in silico stage consisted of docking three-dimensional models of the studied compounds with a three-dimensional model of the leading metabolizing isoform (CYP3A4), which was designated during the in vitro part of the study. XenoSite P450 metabolism prediction was also used to predict sites of metabolism and calculate probability values.Results:The calculated affinities showed a clear similarity when the in vitro results were compared with the calculated in silico affinity values. XenoSite CYP3A4 metabolism probability values also confirm significant participation of CYP3A4 in metabolism of studied compounds.Conclusion:Both molecular docking and XenoSite P450 metabolism prediction provide data that stands in agreement with in vitro studies, granting a more detailed spectrum on predicting CYP3A4 metabolism, and presenting molecular docking as a promising tool to cut costs and increase effectiveness in early drug development stages.