bluuebunny/crossref_metadata_2025_split huggingface dataset:
Social-Scientific Research Competency
Authors: Gess Christopher , Geiger Christoph , Ziegler Matthias 
Published: February 2018

Abstract. Although the development of research competency is an important goal of higher education in social sciences, instruments to measure this outcome often depend on the students’ self-ratings. To provide empirical evidence for the utility of a newly developed instrument for the objective measurement of social-scientific research competency, two validation studies across two independent samples were conducted. Study 1 ( n = 675) provided evidence for unidimensionality, expected differences in test scores between differently advanced groups of students as well as incremental validities over and above self-perceived research self-efficacy. In Study 2 ( n = 82) it was demonstrated that the competency measured indeed is social-scientific and relations to facets of fluid and crystallized intelligence were analyzed. Overall, the results indicate that the test scores reflected a trainable, social-scientific, knowledge-related construct relevant to research performance. These are promising results for the application of the instrument in the evaluation of research education courses in higher education.
NIT1 suppresses tumour proliferation by activating the TGFβ1–Smad2/3 signalling pathway in colorectal cancer
Authors: Lin Chun , Zhang Jianming , Lu Yanxia , Li Xiaomin , Zhang Wenjuan , Zhang Wei , Lin Weihao , Zheng Lin , Li Xuenong 
Published: February 2018

AbstractNIT1 protein has been reported to be a potential tumour suppressor in tumour progression. However, little is known about the specific role of NIT1 in tumour development and progression. In this study, we confirmed the specific effects of NIT1 in the regulation of colorectal carcinoma cell proliferation. Here, we showed that NIT1 was significantly downregulated in colorectal cancer tissues compared with that in adjacent normal tissues. The decreased expression of NIT1 was significantly correlated with poor differentiation and more serosal invasion. Functional experiments showed that NIT1 inhibited CRC cell growth both in vitro and in vivo. NIT1 induced cell cycle arrest and apoptosis. Furthermore, NIT1 recruited Smad2/3 to the TGFβ receptor and activated the TGFβ–Smad2/3 pathway by interacting with SARA and SMAD2/3 in CRC. Further study has shown that SMAD3 directly binds to the promoter regions of NIT1 and enhances the transcription of NIT1. Together, our findings indicate that NIT1 suppresses CRC proliferation through a positive feedback loop between NIT1 and activation of the TGFβ–Smad signalling pathway. This study might provide a new promising strategy for CRC.
Ocean submesoscales as a key component of the global heat budget
Authors: Su Zhan , Wang Jinbo , Klein Patrice , Thompson Andrew F. , Menemenlis Dimitris 
Published: February 2018

AbstractRecent studies highlight that oceanic motions associated with horizontal scales smaller than 50 km, defined here as submesoscales, lead to anomalous vertical heat fluxes from colder to warmer waters. This unique transport property is not captured in climate models that have insufficient resolution to simulate these submesoscale dynamics. Here, we use an ocean model with an unprecedented resolution that, for the first time, globally resolves submesoscale heat transport. Upper-ocean submesoscale turbulence produces a systematically-upward heat transport that is five times larger than mesoscale heat transport, with winter-time averages up to 100 W/m2 for mid-latitudes. Compared to a lower-resolution model, submesoscale heat transport warms the sea surface up to 0.3 °C and produces an upward annual-mean air–sea heat flux anomaly of 4–10 W/m2 at mid-latitudes. These results indicate that submesoscale dynamics are critical to the transport of heat between the ocean interior and the atmosphere, and are thus a key component of the Earth’s climate.
Programmed hierarchical patterning of bacterial populations
Authors: Boehm Christian R. , Grant Paul K. , Haseloff Jim 
Published: February 2018

AbstractModern genetic tools allow the dissection and emulation of fundamental mechanisms shaping morphogenesis in multicellular organisms. Several synthetic genetic circuits for control of multicellular patterning have been reported to date. However, hierarchical induction of gene expression domains has received little attention from synthetic biologists, despite its importance in biological self-organization. Here we report a synthetic genetic system implementing population-based AND-logic for programmed autonomous induction of bacterial gene expression domains. We develop a ratiometric assay for bacteriophage T7 RNA polymerase activity and use it to systematically characterize different intact and split enzyme variants. We then utilize the best-performing variant to build a three-color patterning system responsive to two different homoserine lactones. We validate the AND gate-like behavior of this system both in cell suspension and in surface culture. Finally, we use the synthetic circuit in a membrane-based spatial assay to demonstrate programmed hierarchical patterning of gene expression across bacterial populations.
Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin
Authors: Duchamp de Lageneste Oriane , Julien Anaïs , Abou-Khalil Rana , Frangi Giulia , Carvalho Caroline , Cagnard Nicolas , Cordier Corinne , Conway Simon J. , Colnot Céline 
Published: February 2018

AbstractBone regeneration relies on the activation of skeletal stem cells (SSCs) that still remain poorly characterized. Here, we show that periosteum contains SSCs with high bone regenerative potential compared to bone marrow stromal cells/skeletal stem cells (BMSCs) in mice. Although periosteal cells (PCs) and BMSCs are derived from a common embryonic mesenchymal lineage, postnatally PCs exhibit greater clonogenicity, growth and differentiation capacity than BMSCs. During bone repair, PCs can efficiently contribute to cartilage and bone, and integrate long-term after transplantation. Molecular profiling uncovers genes encoding Periostin and other extracellular matrix molecules associated with the enhanced response to injury of PCs. Periostin gene deletion impairs PC functions and fracture consolidation. Periostin-deficient periosteum cannot reconstitute a pool of PCs after injury demonstrating the presence of SSCs within periosteum and the requirement of Periostin in maintaining this pool. Overall our results highlight the importance of analyzing periosteum and PCs to understand bone phenotypes.
scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells
Authors: Clark Stephen J. , Argelaguet Ricard , Kapourani Chantriolnt-Andreas , Stubbs Thomas M. , Lee Heather J. , Alda-Catalinas Celia , Krueger Felix , Sanguinetti Guido , Kelsey Gavin , Marioni John C. , Stegle Oliver , Reik Wolf 
Published: February 2018

AbstractParallel single-cell sequencing protocols represent powerful methods for investigating regulatory relationships, including epigenome-transcriptome interactions. Here, we report a single-cell method for parallel chromatin accessibility, DNA methylation and transcriptome profiling. scNMT-seq (single-cell nucleosome, methylation and transcription sequencing) uses a GpC methyltransferase to label open chromatin followed by bisulfite and RNA sequencing. We validate scNMT-seq by applying it to differentiating mouse embryonic stem cells, finding links between all three molecular layers and revealing dynamic coupling between epigenomic layers during differentiation.
A non-invasive method to directly quantify surface heterogeneity of porous materials
Authors: Chiang Wei-Shan , Georgi Daniel , Yildirim Taner , Chen Jin-Hong , Liu Yun 
Published: February 2018

AbstractIt is extremely challenging to measure the variation of pore surface properties in complex porous systems even though many porous materials have widely differing pore surface properties at microscopic levels. The surface heterogeneity results in different adsorption/desorption behaviors and storage capacity of guest molecules in pores. Built upon the conventional Porod’s law scattering theory applicable mainly to porous materials with relatively homogeneous matrices, here we develop a generalized Porod’s scattering law method (GPSLM) to study heterogeneous porous materials and directly obtain the variation of scattering length density (SLD) of pore surfaces. As SLD is a function of the chemical formula and density of the matrix, the non-invasive GPSLM provides a way to probe surface compositional heterogeneity, and can be applied to a wide range of heterogeneous materials especially, but not limited to, porous media and colloids, using either neutron or X-ray scattering techniques.
Painting with light-powered bacteria
Authors: Arlt Jochen , Martinez Vincent A. , Dawson Angela , Pilizota Teuta , Poon Wilson C. K. 
Published: February 2018

AbstractSelf-assembly is a promising route for micro- and nano-fabrication with potential to revolutionise many areas of technology, including personalised medicine. Here we demonstrate that external control of the swimming speed of microswimmers can be used to self assemble reconfigurable designer structures in situ. We implement such ‘smart templated active self assembly’ in a fluid environment by using spatially patterned light fields to control photon-powered strains of motile Escherichia coli bacteria. The physics and biology governing the sharpness and formation speed of patterns is investigated using a bespoke strain designed to respond quickly to changes in light intensity. Our protocol provides a distinct paradigm for self-assembly of structures on the 10 μm to mm scale.
Kinetics of lithium peroxide oxidation by redox mediators and consequences for the lithium–oxygen cell
Authors: Chen Yuhui , Gao Xiangwen , Johnson Lee R. , Bruce Peter G. 
Published: February 2018

AbstractLithium–oxygen cells, in which lithium peroxide forms in solution rather than on the electrode surface, can sustain relatively high cycling rates but require redox mediators to charge. The mediators are oxidised at the electrode surface and then oxidise lithium peroxide stored in the cathode. The kinetics of lithium peroxide oxidation has received almost no attention and yet is crucial for the operation of the lithium–oxygen cell. It is essential that the molecules oxidise lithium peroxide sufficiently rapidly to sustain fast charging. Here, we investigate the kinetics of lithium peroxide oxidation by several different classes of redox mediators. We show that the reaction is not a simple outer-sphere electron transfer and that the steric structure of the mediator molecule plays an important role. The fastest mediator studied could sustain a charging current of up to 1.9 A cm–2, based on a model for a porous electrode described here.
Quantum phase transitions in highly crystalline two-dimensional superconductors
Authors: Saito Yu , Nojima Tsutomu , Iwasa Yoshihiro 
Published: February 2018

AbstractSuperconductor–insulator transition is one of the remarkable phenomena driven by quantum fluctuation in two-dimensional (2D) systems. Such a quantum phase transition (QPT) was investigated predominantly on highly disordered thin films with amorphous or granular structures using scaling law with constant exponents. Here, we provide a totally different view of QPT in highly crystalline 2D superconductors. According to the magneto-transport measurements in 2D superconducting ZrNCl and MoS2, we found that the quantum metallic state commonly observed at low magnetic fields is converted via the quantum Griffiths state to the weakly localized metal at high magnetic fields. The scaling behavior, characterized by the diverging dynamical critical exponent (Griffiths singularity), indicates that the quantum fluctuation manifests itself as superconducting puddles, in marked contrast to the thermal fluctuation. We suggest that an evolution from the quantum metallic to the quantum Griffiths state is generic nature in highly crystalline 2D superconductors with weak pinning potentials.
Micro-pharmacokinetics: Quantifying local drug concentration at live cell membranes
Authors: Gherbi Karolina , Briddon Stephen J. , Charlton Steven J. 
Published: February 2018

AbstractFundamental equations for determining pharmacological parameters, such as the binding affinity of a ligand for its target receptor, assume a homogeneous distribution of ligand, with concentrations in the immediate vicinity of the receptor being the same as those in the bulk aqueous phase. It is, however, known that drugs are able to interact directly with the plasma membrane, potentially increasing local ligand concentrations around the receptor. We have previously reported an influence of ligand-phospholipid interactions on ligand binding kinetics at the β2-adrenoceptor, which resulted in distinct “micro-pharmacokinetic” ligand profiles. Here, we directly quantified the local concentration of BODIPY630/650-PEG8-S-propranolol (BY-propranolol), a fluorescent derivative of the classical β-blocker propranolol, at various distances above membranes of single living cells using fluorescence correlation spectroscopy. We show for the first time a significantly increased ligand concentration immediately adjacent to the cell membrane compared to the bulk aqueous phase. We further show a clear role of both the cell membrane and the β2-adrenoceptor in determining high local BY-propranolol concentrations at the cell surface. These data suggest that the true binding affinity of BY-propranolol for the β2-adrenoceptor is likely far lower than previously reported and highlights the critical importance of understanding the “micro-pharmacokinetic” profiles of ligands for membrane-associated proteins.
Citalopram inhibits platelet function independently of SERT-mediated 5-HT transport
Authors: Roweth Harvey G. , Yan Ruoling , Bedwani Nader H. , Chauhan Alisha , Fowler Nicole , Watson Alice H. , Malcor Jean-Daniel , Sage Stewart O. , Jarvis Gavin E. 
Published: February 2018

AbstractCitalopram prevents serotonin (5-HT) uptake into platelets by blocking the serotonin reuptake transporter (SERT). Although some clinical data suggest that selective serotonin reuptake inhibitors (SSRIs) may affect haemostasis and thrombosis, these poorly-characterised effects are not well understood mechanistically and useful in vitro data is limited. We sought to determine whether the inhibitory effects of citalopram on platelets are mediated via its pharmacological inhibition of 5-HT transport. We quantified the inhibitory potency of (RS)-, (R)- and (S)-citalopram on platelet function. If SERT blockade is the primary mechanism for citalopram-mediated platelet inhibition, these potencies should show quantitative congruence with inhibition of 5-HT uptake. Our data show that citalopram inhibits platelet aggregation, adhesion and thromboxane production with no difference in potency between (R)- and (S)-isomers. By contrast, citalopram had a eudysmic ratio of approximately 17 (S > R) for SERT blockade. Furthermore, nanomolar concentrations of citalopram inhibited 5-HT uptake into platelets but had no effect on other platelet functions, which were inhibited by micromolar concentrations. Our data indicate that citalopram-induced inhibition of platelets in vitro is not mediated by blockade of 5-HT transport. This raises a new question for future investigation: by what mechanism(s) does citalopram inhibit platelets?
Enhancement of methane production from Cotton Stalk using different pretreatment techniques
Authors: Zhang Han , Ning Zhifang , Khalid Habiba , Zhang Ruihong , Liu Guangqing , Chen Chang 
Published: February 2018

AbstractChina produces large amount of cotton stalk (CS) residues as agricultural biomass, which are incinerated on-site, causing air pollution. The high organic content of CS could be utilized for biogas production, but the direct digestion without pretreatment always leads to a low methane yield and biodegradability, due to the complicated structure of lignocellulose. In order to search best fitting pretreatment methods in effective anaerobic digestion (AD) of CS, effects of various pretreatments including KOH, NaOH, Ca(OH)2, alkali hydrogen peroxide (AHP), H2SO4, H3PO4and steam explosion (SE) were studied. It was seen that all treatments resulted in varying methane yields. Among all the pretreatments, acid pretreatment is not suitable for AD of CS. The results showed that the highest cumulative methane yield (CMY) of 192.4 mL·gVS−1was obtained after 3% AHP pretreatment of CS, and the methane yield improved by 254.3% than the untreated CS. Therefore, AHP treatment was proven to be an efficient pretreatment technique. XRD and FTIR analyses had shown that pretreated CS had favorable structural changes. This research is beneficial in developing environment friendly and cost-effective pretreatment technologies to utilize CS for methane production in future application.
HPV-transformed cells exhibit altered HMGB1-TLR4/MyD88-SARM1 signaling axis
Authors: Morale Mirian Galliote , da Silva Abjaude Walason , Silva Aline Montenegro , Villa Luisa Lina , Boccardo Enrique 
Published: February 2018

AbstractCervical cancer is one of the leading causes of cancer death in women worldwide. Persistent infection with high-risk human papillomavirus (HPV) types is the main risk factor for the development of cervical cancer precursor lesions. HPV persistence and tumor development is usually characterized by innate immune system evasion. Alterations in Toll-like receptors (TLR) expression and activation may be important for the control of HPV infections and could play a role in the progression of lesions and tumors. In the present study, we analyzed the mRNA expression of 84 genes involved in TLR signaling pathways. We observed that 80% of the differentially expressed genes were downregulated in cervical cancer cell lines relative to normal keratinocytes. Major alterations were detected in genes coding for several proteins of the TLR signaling axis, including TLR adaptor molecules and genes associated with MAPK pathway, NFκB activation and antiviral immune response. In particular, we observed major alterations in the HMGB1-TLR4 signaling axis. Functional analysis also showed that HMGB1 expression is important for the proliferative and tumorigenic potential of cervical cancer cell lines. Taken together, these data indicate that alterations in TLR signaling pathways may play a role in the oncogenic potential of cells expressing HPV oncogenes.
Wavelength Assignment in Hybrid Quantum-Classical Networks
Authors: Bahrani Sima , Razavi Mohsen , Salehi Jawad A. 
Published: February 2018

AbstractOptimal wavelength assignment in dense-wavelength-division-multiplexing (DWDM) systems that integrate both quantum and classical channels is studied. In such systems, weak quantum key distribution (QKD) signals travel alongside intense classical signals on the same fiber, where the former can be masked by the background noise induced by the latter. Here, we investigate how optimal wavelength assignment can mitigate this problem. We consider different DWDM structures and various sources of crosstalk and propose several near-optimal wavelength assignment methods that maximize the total secret key rate of the QKD channels. Our numerical results show that the optimum wavelength assignment pattern is commonly consisted of several interspersed quantum and classical bands. Using our proposed techniques, the total secret key rate of quantum channels can substantially be improved, as compared to conventional assignment methods, in the noise dominated regimes. Alternatively, we can maximize the number of QKD users supported under certain key rate constraints.
Ultrastructural and Immunohistochemical Features of Telocytes in Placental Villi in Preeclampsia
Authors: Nizyaeva Natalia V. , Sukhacheva Tatiana V. , Serov Roman A. , Kulikova Galina V. , Nagovitsyna Marina N. , Kan Natalia E. , Tyutyunnik Victor L. , Pavlovich Stanislav V. , Poltavtseva Rimma A. , Yarotskaya Ekaterina L. , Shchegolev Aleksandr I. , Sukhikh Gennadiy T. 
Published: February 2018

AbstractA new cell type, interstitial Cajal-like cell (ICLC), was recently described in different organs. The name was recently changed to telocytes (TCs), and their typical thin, long processes have been named telopodes (Tp). TCs regulate the contractile activity of smooth muscle cells and play a role in regulating vessel contractions. Although the placenta is not an innervated organ, we believe that TCs are present in the placenta. We studied placenta samples from physiological pregnancies and in different variants of preeclampsia (PE). We examined these samples using light microscopy of semi-thin sections, transmission electron microscopy, and immunohistochemistry. Immunohistochemical examination was performed with primary antibodies to CD34, CD117, SMA, and vimentin, and TMEM16a (DOG-1), the latter was used for the diagnosis of gastrointestinal stromal tumours (GIST) consisting of TCs. We have identified a heterogenetic population of ТСs in term placentas, as these cell types differed in their localization, immunophenotype and ultrastructural characteristics. We assume TMEM16a could be used as the marker for identification of TCs. In PE we have revealed telocyte-like cells with ultrastructural signs of fibrocytes (significant process thickening and the granular endoplasmic reticulum content was increased) and a loss of TMEM16a immunohistochemical staining.
Antitumor activities of Quercetin and Green Tea in xenografts of human leukemia HL60 cells
Authors: Calgarotto Andrana Karla , Maso Victor , Junior Gilberto Carlos Franchi , Nowill Alexandre Eduardo , Filho Paulo Latuf , Vassallo José , Saad Sara Teresinha Olalla 
Published: February 2018

AbstractQuercetin is one of the most abundant flavonoids, present in fruits and vegetables and has been shown to have multiple properties capable of reducing cell growth in cancer cells. Green tea is a widely consumed beverage, known for a potential source of free radical scavenging and anti-cancer activities. Herein, we investigate the in vivo antitumor efficacy of quercetin and green tea in human leukemia. Human tumors were xenografted into NOD/SCID mice. Quercetin and green tea reduced tumor growth in HL-60 xenografts accompanied by decreased expression of anti-apoptotic proteins, BCL-2, BCL-XL and MCL-1 and increased expression of BAX, a pro-apoptotic protein. Moreover, caspase-3 was activated to a greater extent after quercetin and green tea treatment. Quercetin and green tea also mediated G1 phase cell cycle arrest in HL-60 xenografts. Treatment with quercetin and green tea induced conversion of LC3-I to LC3-II as well as activation of autophagy proteins, suggesting that quercetin and green tea initiate the autophagic progression. We have provided evidence that quercetin and green tea induces signaling at the level of apoptosis, cell cycle and autophagy which converge to antigrowth effects in HL-60 xenograft mice suggesting that these compounds may be a compelling ally in cancer treatment.