bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Adjacent NNRTI Binding Pocket in Multi-mutated HIV1- RT Enzyme Model: An in silico Study
Authors: Kamil R.F. , Debnath U. , Verma S. , Prabhakar Y.S. 
Published: April 2018

Introduction:
A possible strategy to combat mutant strains is to have a thorough structural
evaluation before and after mutations to identify the diversity in the non-nucleoside inhibitor binding
pocket and their effects on enzyme-ligand interactions to generate novel NNRTI’s accordingly.

Objective:
The primary objective of this study was to find effects of multiple point mutations on
NNRTI binding pocket. This study included the contribution of each individual mutation in NNIBP
that propose an adjacent binding pocket which can be used to discover novel NNRTI derivatives.

Methods:
An in Silico model of HIV-1 RT enzyme with multiple mutations K103N, Y181C and
Y188L was developed and evaluated. Two designed NNRTI pyridinone derivatives were selected as
ligands for docking studies with the homology model through alignment based docking and residue
based docking approaches. Binding pockets of wild type HIV-1 RT and multi-mutated homology
model were compared thoroughly.

Result and Discussion:
K103N mutation narrowed the entrance of NNRTI binding pocket and forbade
electrostatic interaction with α amino group of LYS103. Mutations Y181C and Y188L prevented
NNRTI binding by eliminating aromatic π interactions offered by tyrosine rings. Docking
study against new homology model suggested an adjacent binding pocket with combination of residues
in palm and connection domains. This pocket is approximately 14.46Å away from conventional
NNRTI binding site.

Conclusion:

Increased rigidity, steric hindrance and losses of important interactions cumulatively
prompt ligands to adapt adjacent NNRTI binding pocket. The proposed new and adjacent binding
pocket is identified by this study which can further be evaluated to generate novel derivatives.
Stigma, Stigmatisasi, Perilaku Kekerasan dan Ketakutan diantara Orang dengan Gangguan Jiwa (ODGJ) di Indonesia: Penelitian Constructivist Grounded theory
Authors: Subu Muhammad Arsyad , Waluyo Imam , Nurdin Adnil Edwin , Priscilla Vetty , Aprina Tilawaty 
Published: April 2018

Perilaku kekerasan dan perasaan ketakutan serta stigma dan proses stigmatisasi terkait orang dengan gangguan jiwa (ODGJ) banyak dilaporkan. Penelitian ini bertujuan memahami dampak stigma dan proses stigmatisasi terhadap perilaku kekerasan oleh ODGJ dan perilaku kekerasan terhadap ODGJ dan untuk mengetahui ketakutan yang dirasakan oleh ODG serta ketakutan orang lain terhadap ODGJ. Penelitian menggunakan metode Charmaz Constructivist Grounded Theory (CCGT) dengan 30 pasien dan perawat yang bekerja di RS. Marzoeki Mahdi Bogor sebagai partisipan. Metode pengumpulan data dilakukan dengan wawancara semi-terstruktur, dokumen review, memo dan catatan lapangan dan dianalisis dengan Paille grounded theory analisis. Penelitian ini menghasilkan dua tema utama: 1) perilaku kekerasan dan 2) perasaan ketakutan; serta sembilan sub-kategori. Hasil penelitian menunjukkan bahwa perilaku kekerasan dilakukan oleh pasien termasuk terhadap diri sendiri, keluarga, masyarakat, dan tenaga kesehatan. Kekerasan juga dialami oleh penderita dari orang lain. Akibat stigmatisasi, ketakutan dirasakan oleh penderita dan orang lain tehadap penderita. Dampak stigmatisasi dimanifestasikan dengan perilaku kekerasan dan ketakutan yang dialami oleh penderita sendiri, keluarga, dan masyarakat.Â Â
Kinetic investigation of emulsion stability in oil/water emulsions stabilized with protein
Authors: Gandova Vanya 
Published: April 2018

The emulsion stability soybean oil/water (O/W) with different concentrations was investigated. The dynamics of emulsion stabilities were investigated at 1 to 15 days as measured of turbidity. Three series of samples were prepared with 2 %, 4 % and 5 % soybean protein isolates (SI). Obtained results were compared with other obtained with optical microscope. It was found that emulsions exhibited more stability in samples with 2 % SI. The pH was measured in all emulsions. pH interval was between 5.8–7.4and after analysis were seen that emulsions exhibited high stability around 7.2-7.3 pH values.
The Influence of Compensation and Work Environment towards Teacherâ€™s Job Satisfaction
Authors: Rahayuningsih Sri 
Published: April 2018

This study focus on the teacher satisfaction and work style based on the compensation given. This study aims; (1) to know the effect of compensation towards teachersâ€™ work satisfaction, (2) to know the effect of work environment towards teachersâ€™ work satisfaction, (3) to know the effect ofÂ compensation and work environment simultaneouslyÂ towards teachersâ€™ work satisfaction. The data analysis used in this research were descriptive analysis, pre-requirement test analysis, and hypothesis test including simple linier regression analysis that was used to know the effect of compensation towards teachersâ€™ work satisfaction and the effect of work environment towards teachersâ€™ work satisfaction and multiple regression analysis to know the effect of compensation and work environment simultaneouslyÂ towards teachersâ€™ work satisfaction. Â From the hypothesis testing, it is found that; (1) there was a positive effect of compensation towards teachersâ€™ work satisfaction which revealed by Y=55.501 + 0.585 X1 with the correlation coefficient as 0.834 with contribution about 0.695 or 6905%, (2) ) there is a positive effect of work environment towards teachersâ€™ work satisfaction which revealed by Y=56.157 + 0.585 X2 with the correlation coefficient as 0.740 with contribution about 0.547 or 54.7%, (3) there is a positive effect ofÂ compensation and work environment simultaneouslyÂ towards teachersâ€™ work satisfaction which revealed by Y=39.414 + 0.427 X1 + 0.299 X2 with the correlation coefficient as 0.887 with contribution about 0.788 or 78.8%.To increase teachersâ€™ work satisfaction, there must be the increase of compensation as the appreciation for teachersâ€™ performance. Besides, a conducive work environment must be increased to increase teachersâ€™ work satisfaction which will contribute to the increase of education quality
Vídeo Juegos Educativos desde la Perspectiva de la Educación para Todos
Authors: Zapata Lesmes Clemencia , Barro Hernández Alba Rosa , Suárez Contreras Ludis María 
Published: April 2018

Este estudio es parte del macroproyecto Modelo para producción de contenidos digitales educativos para inclusión de sordos, adscrito al Programa Nacional Arquitectura Pedagógica, didáctica y tecnológica para formación de profesores en y para la diversidad, financiado por COLCIENCIAS mediante contrato 546-2014. En este proyecto se presentó el videojuego desde el punto de vista educativo mostrando sus ventajas y desventajas como oportunidad para potencializar los procesos de enseñanza aprendizaje en la población sorda. Para ello, se llevó a cabo una investigación documental, una fase heurística sobre investigaciones realizadas por expertos en el tema de la educación para todos y el uso de los videojuegos, y, otra hermenéutica, para sintetizar la información en una cuadrícula que menciona las diferentes ventajas y desventajas de los videojuegos educativos para la población infantil y adolescente.. Asimismo, se reconoce la necesidad de las escuelas de poseer una infraestructura y soporte en hardware y software necesarios para la aplicación del video juego educativo. Como resultado se establece una secuencia didáctica para uso del video juego educativo como estrategia educativa que contribuya al desarrollo de habilidades y capacidades en el proceso de enseñanza aprendizaje de los sordos y oyentes.
Elementos para la discusión sobre la gobernanza y gobernabilidad en el posconflicto en Colombia
Authors: Buelvas Mendoza Milton , Florez Campo Oscar 
Published: April 2018

En el presente artículo de reflexión se dará cuenta que el éxito del posconflicto concurren diversas variables que si se logran materializar permitirán reafirmar la paz y su permanencia en el tiempo. Podemos destacar entre esas variables, el cumplimiento de los Acuerdos pactados entre gobierno e insurgencia, y para eso se necesitará voluntad política de las partes, y los recursos económicos que sostengan lo acordado. Que la Justicia Especial para la Paz cumpla su cometido y logre garantizar justicia, reparación y no repetición. Estos aspectos significan que los gobernantes deben estar dispuestos a impulsar la cristalización de lo pactado y para ello será indispensable gobernanza y gobernabilidad.

Palabras Claves:
Posconflicto, pos acuerdos, Gobernanza, gobernabilidad, institucionalidad, posverdad, justicia, paz
Trabajo social frente a la construcción del género y la influencia de la multiculturalidad.
Authors: Del Rio Durango Jennifer 
Published: April 2018

En este artículo de investigación se analizarán y cuestionarán diferentes representaciones del género en el contexto multicultural de la ciudad de Cartagena. En tal orden de ideas, plantea una concepción compleja de la identidad de género, pues explora cómo las construcciones sociales de lo femenino: a) producen violencias de diversa naturaleza; b) son fomentadas mediante instituciones (escuela, religión, familia); y c) están ligadas a otros aspectos como la raza, la clase social y el lugar de origen. Por otro lado, la investigación alude a una serie de discursos (normativos, teóricos) y prácticas que permiten reformular los esquemas anteriores, con el fin de promover nuevas formas de libertad, tolerancia y resistencia.
Experimental Investigation of Tire Slap Noise
Authors: Cao Rui , Bolton J. Stuart 
Published: April 2018

ABSTRACT

Tire noise is an important issue both in the vehicle interior and to the vehicle exterior, since it affects passenger comfort and environmental noise levels, respectively. Such noise is increased when a tire encounters discontinuities on the road surface, the discontinuity being either a gap or a bump. The relatively high frequency (e.g., approximately 1 kHz and above) airborne tire noise generated by such discontinuities is defined as tire slap noise in this study.
Most previous research on noise generated by surface discontinuities has been focused on lower frequency tire noise, typically below 600 Hz, and, in particular, on structural-borne noise transmitted from the tire into the vehicle associated with the acoustic modes of the tire interior. Here, instead, the focus is on higher frequency airborne transmission. Further, the study here is conducted from the perspective of tire structural vibration, which concerns the vibration of and sound radiation from the tire treadband structure, rather than tire pattern noise, for example.
The high frequency tire slap noise was investigated in a laboratory environment. The measurements were conducted by using the Ray W. Herrick Laboratories' Tire Pavement Test Apparatus (TPTA), on which a loaded tire can be run on realistic road surfaces at speeds up to 50 km/h; the resulting tire noise was measured using the On-Board Sound Intensity (OBSI) method. A gap between two different concrete surfaces was chosen as the road discontinuity, and both narrow band and one-third-octave band spectra were recorded over the gap and over the adjacent smooth road surface segments.
Several tires were tested on the TPTA, and their slap noise was recorded. Surface noise and slap noise were compared up to 1600 Hz to show the impact of the discontinuity on the tire noise radiation. Generally, slap noise is of a higher level than the surface noise, especially between 800 and 1400 Hz, but some tires showed distinct differences between the noise response on the surface and over the gap, while other tires radiated similar noise on both the surface and over the gap. Moreover, static tire mobility measurements were performed to investigate the wave type responsible for the different responses on the gap and the surface.
Uniaxial Compression on Salted Snow
Authors: Giudici Henri , Wåhlin Johan , Klein-Paste Alex 
Published: April 2018

ABSTRACT

During snowfall loose snow sticks to the underlying pavement, forming a hard crust that can be difficult to remove. In order to ensure good road traffic safety, winter maintenance personnel use several operations, such as mechanical removal and chemical altering, to keep snow plowable. One chemical, commonly applied on roads, is sodium chloride (salt). Recent studies establish that very small amounts of salt are sufficient to weaken compacted snow substantially, meaning that the mechanical properties of salted snow are different from dry snow. This change in the mechanical properties of salted snow, however, has not been considered when studying tire–pavement interaction in snowy conditions.
In an attempt to better understand salt snow as a material, this paper presents an experimental investigation of salted snow under uniaxial compression. The study examines the density and the loss of mass of snow after compression for different solution contents and compression speeds.
Samples of snow were mixed with sodium chloride solution at different liquid contents. The snow samples were then compressed by an MTS uniaxial compression machine, up to an applied load of approximately 2 bars, roughly corresponding to the pressure applied by a car. Applied forces and dry density were measured for each test after compression. Based on the results carried out in this study, we found that: Salted snow gets a higher density after compaction compared to dry snow, also flows easier than dry snow, giving a larger loss of mass during uniaxial compression. The dry density after compaction increases with compaction speed for dry snow but decreases with compaction speed for salted snow.
The effect of the solution on the compressibility and flowability of the material and its relevance for tire–pavement interaction are discussed.
HUBUNGAN KEYAKINAN DIRI MENGELOLA KONFLIK KERJA-KELUARGA, DUKUNGAN ATASAN, DUKUNGAN KELUARGA DENGAN PENGAYAAN KERJA-KELUARGA
Authors: Artiawati Artiawati , Astutik Neni Puji 
Published: April 2018

Perkembangan jaman mengakibatkan banyak terjadi pergeseran dalam bidang kehidupan, salah satunya adalah terjadinya pergeseran peran dalam rumah tangga, seperti semakin banyaknya pasangan dual karir. Dual karir tentunya akan menimbulkan tantangan tersendiri dalam pengelolaan domain kerja dan rumah tangga. Penelitian ini bertujuan untuk menguji hubungan keyakinan diri mengelola konflik kerja-keluarga, dukungan atasan dan dukungan keluarga besar dengan pengayaan kerja-keluarga. Partisipan penelitian ini sebanyak 250 guru di Surabaya. Penelitian ini menggunakan pendekatan kuantitatif dan pengumpulan data melalui kuisioner. Alat ukur yang digunakan dalam penelitian ini, yaitu kuesioner pengayaan kerja-keluarga dibuat oleh Carlson, Kacmar, Waybe & Grzywacz (2006) dan keyakinan diri mengelola konflik kerja-keluarga dibuat oleh Cinamon (2003) yang diadaptasi oleh Artiawati dan Andhini (2016). Kuesioner dukungan atasan dan dukungan keluarga besar yang dibuat oleh Antani dan Ayman (2003) dengan tim project 3535 dan didaptasi oleh Artiawati (2012). Data yang diperoleh diolah menggunakan SPSS dengan teknik analisis regresi ganda. Hasil yang didapatkan,antara lain:(1)ada hubungan yang signifikan antara keyakinan diri mengelola konflik kerja-keluarga, dukungan atasan, dan dukungan keluarga besar dengan pengayaan kerja-keluarga (F = 25.032; p < 0.05; R square = 0.234). (2) keyakinan diri mengelola konflik kerja-keluarga memiliki hubungan paling kuat dengan pengayaan kerja-keluarga (r parsial 0.259 ; p < 0,05). Saran untuk penelitian selanjutnya perlu melibatkan variabel lain seperti kepribadian, konflik kerja-keluarga, juga mempertimbangkan latar belakang kultural. Kata kunci: keyakinan diri mengelola konflik kerja-keluarga, dukungan keluarga besar, dukungan atasan, guru.
Safety and immunogenicity of rabies pre- and postexposure intradermal regimens using Abhayrab, a purified vero cell rabies vaccine (PVRV) produced in India in healthy volunteers: towards greater affordability of rabies prophylaxis
Authors: Magpantay Rio L. , Bermal Nancy , Medina Plebeian , Quiambao Beatriz P. 
Published: April 2018

Abstract
Background: Rabies is 100% fatal, but is preventable. However, due to the high cost of vaccines and immunoglobulins, as well as their limited availability, many dog-bite victims in the Philippines do not complete the required PEP regimen. This concern may be addressed by the introduction of various new brands of affordable rabies vaccines and biologicals for use with the ID regimen. Objective: Study the safety and immunogenicity of rabies pre- and post-exposure intradermal regimens using Abhayrab, a purified vero cell rabies vaccine (PVRV) produced by the human biological institute of Ooty India in healthy volunteers. Methods: In a randomized, single blind, unicentric trial, 149 healthy volunteers (5 - 50 years in age) were recruited to the study. The subjects were then randomized into two groups. Group 1 (pre-exposure) (73 subjects) followed the standard ID regimen using a dose of 0.1 mL PVRV (Abhayrab) given intradermally on either deltoid on days 0, 7 and 28. Group 2 (post-exposure) (76 Subjects) followed the modified Thai Red Cross ID regimen (2-2-2-0-2) using a dose of 0.1 mL PVRV (Abhayrab) each given intradermally on both deltoids on days 0, 3, 7, and 28. Following administration of vaccine, subjects were observed closely for 30 minutes at the site for adverse reactions. Four ml of blood from each subject was drawn on days 0, 14, and 28 for anti-rabies antibody titres. Safety and immunogenicity were assessed through follow-up of adverse events and anti rabies antibody response, respectively. Results: Eventually, 120 subjects, 60 in each group, completed the day 28 ID immunization. All the 120 subjects demonstrated seroconversions with antibody titers greater than the WHO recommended cut-off level of 0.5 IU/mL on days 14 and 28. The GMC values for Group 1 were 3.30 IU/ml and 4.37 IU/mL on days 14 and 28, respectively, while those of Group 2 were 3.73 IU/mL and 4.82 IU/mL, respectively. These GMC values were within their 95% CIs. Only a few mild adverse events were observed with no incidences of moderate or severe events. Conclusion: Abhayrab is a safe and immunogenic rabies vaccine when administered intradermally.
Walk performance in Thai men and women: physical activity dependence
Authors: Suwanachaiy Sitamanats , Kulaputana Onanong , Chaiwanichsiri Dootchai 
Published: April 2018

Abstract
Background: Six-minute walk test (6MWT) is a measure of functional capacity and a predictor of morbidity and mortality in the elderly. Published reference equations for the six-minute walk distance (6MWD) were derived from healthy subjects with sufficient physical activity, and may not be valid for patients with limited physical activity. Objective: To measure 6MWD in healthy individuals with sufficient (Suff) and insufficient (Insuff) levels of physical activity in Thai, and to compare the measured 6MWD with those calculated using published reference equations. Methods: Healthy volunteers aged 45-65 years (77 men and 85 women) performed three standard 6MWT. Physical activity levels were determined using a one-week recall physical activity questionnaire. The subjects were classified as having sufficient or insufficient levels of physical activity. Results: 6MWD of the Suff group was greater than the Insuff group in both men (701+89 m vs. 652+55 m, p = 0.005) and women (619+49 m vs. 571+35 m, p <0.001). The predicted 6MWD from published reference equations underestimated the measured 6MWD of men in Suff group, while it overestimated the measured 6MWD of women in Insuff group. Conclusion: Level of physical activity affected 6MWD of the subjects. Published references must be carefully applied for predicting 6MWD of individuals with various levels of physical activity.
Comparing anterior cervical fusion using titanium cage with hydroxyapatite and with autograft
Authors: Tienboon Prakit , Atiprayoon Surapon 
Published: April 2018

Abstract
Background: Anterior cervical discectomy and fusion using autogenous iliac bone graft may lead to donor site pain. This has led some surgeons to use hydroxyapatite but it has greater rates of complications. Objective: Analyze results of the treatment of cervical spine spondylosis by anterior cervical discectomy, and to compare fusion using titanium cage with hydroxyapatite and with autograft. Methods: We evaluated eighty patients (at 146 cervical levels) that had been treated by anterior cervical discectomy and fusion with hydroxyapatite or autograft. In a prospective study, patients were divided into two groups: 1) 77 levels of anterior cervical fusion from in 40 patients who were treated with cage and hydroxyapatite, and 2) 69 levels of anterior cervical fusions in 40 patients who were treated with cage and autograft. Results were evaluated using the Japanese Orthopedic Association (JOA) scoring system before and after surgery, subsidence, non-union and complication. Results: According to JOA score, the first group (hydroxyapatite) had improved from 9.8 preoperation to 14.5 postoperation. The subsidence rate was 26.0%, the non-union rate was 10.4%, and there was no intra and post-operative complication. In the other group (autograft), the preoperative JOA score had improved from 9.3 to 14.1. The subsidence rate was 11.6%, and there were no non-union and complications. Statistical significance was found in non-union and subsidence aspect between groups, but the clinical outcome of JOA score was not significantly different. Conclusion: Anterior cervical discectomy and fusion in cervical spondylosis patients, titanium cage implantation with hydroxyapatite is a highly useful alternative to autograft.
Mutations of ATP7B gene in two Thai siblings with Wilson disease
Authors: Treepongkaruna Suporn , Pienvichit Paneeya , Phuapradit Pornpimon , Kodcharin Porawee , Wattanasirichaigoon Duangrurdee 
Published: April 2018

Abstract
Background: Wilson disease (WD) is an autosomal recessive disorder of copper metabolism caused by mutations in ATP7B gene. Objective: Report the clinical data and mutation analysis of two Thai siblings suspected of WD. Subject and methods: A 13-year-old boy who presented with cirrhosis, arthralgia, hypoalbuminemia, and coagulopathy, and his 11-year-old sister who was asymptomatic but had hepatomegaly with elevation of transaminases, were studied. Mutation analysis of ATP7B gene and mRNA analysis was performed in both patients and their parents. Results: Investigations were consistent with WD, and their liver diseases improved after standard treatment for WD. DNA analyses in these two patients revealed two novel mutations, which were a deletion of the first 2bp of exon 6 (c.1870_1871delGA), and a single base substitution from A to G at nucleotide 4075 (c.4075A>G) in the exon 20 (p.M1359V). PCR-restriction digestion with NcoI restriction enzyme was employed as the second method for confirmation of the c.4075A>G mutation and for rapid screening in 100 chromosomes from unrelated healthy controls, and this variant was not present in the controls. The c.1870_1871delGA deletion caused a frameshift effect, which results in a premature stop codon (p.E624fsX753), and the p.M1359V mutation is a substitution of methionine with valine, which may have effects upon its orientation and interaction with other adjacent amino acids. Conclusion: Two novel mutations of ATP7B gene were identified in two Thai siblings with WD.