bluuebunny/crossref_metadata_2025_split huggingface dataset:
Homo‐ and Heterodimeric Dyes for Dye‐Sensitized Solar Cells: Panchromatic Light Absorption and Modulated Open Circuit Potential
Authors: Sil Manik Chandra , Sudhakar Vediappan , Singh Ambarish Kumar , Kavungathodi Munavvar Fairoos Mele , Nithyanandhan Jayaraj 
Published: September 2018

AbstractThe design of dyes for panchromatic light absorption has attracted much attention in the field of dye‐sensitized solar cells (DSSCs). An approach to enhance panchromatic light absorption utilizes mixtures of complementary light‐absorbing dyes as well as dyes with specific anchoring groups that facilitate interfacial charge transfer with TiO2. Dipole‐dipole interactions between the dye molecules on the surface broaden the spectrum, which results in decreased DSSC device performance. However, controlled aggregation of dyes results in broadening the spectral profile along with enhanced photocurrent generation. To control the dye‐dye interaction, dimeric dyes with different dipole lengths D1‐Dsq, Dsq‐Dsqwere systematically designed and synthesized. The photophysical and electrochemical properties were evaluated and the EHOMO and ELUMO levels were determined; these energy levels determines the electron injection from ELUMO of the dye to ECB of TiO2 and regeneration of oxidized dye by the electrolyte, respectively. The absorption spectra of Dsq‐Dsq, D1‐Dsq were broadened in solution compared to model dye Dsq; this indicates that the dye‐dye interaction is prominent in solution. In D1‐Dsq excitation energy transfer between photoexcited D1 and Dsq was explained by using Förster resonance energy transfer (FRET). The homodimeric dye showed a device performace of 2.8 % (Voc 0.607, Jsc 6.62 mA/cm2, ff 69.3 %),whereas the heterodimeric dye D1‐Dsq showed a device performance of 3.9 % (Voc 0.652 V, Jsc 8.89 mA/cm2, ff 68.8 %). The increased photocurrent for D1‐Dsqis due to the panchromatic IPCE response compared to Dsq‐Dsq. The increased Vocis due to the effective passivation of the TiO2 surface by the spirolinker, and the effective dipole moment that shifts the conduction band on TiO2. Hence, the open circuit potential, Voc, for the devices prepared from Dsq, D1‐Dsq and Dsq‐Dsqwere systematically modulated by controlling the intermolecular π‐π and intramolecular dipole‐dipole interactions of the dimeric dyes.
Driving cognitions, rumination, and posttraumatic stress disorder in road traffic accidents survivors
Authors: Măirean Cornelia 
Published: September 2018

AbstractThis study evaluated the relationships between driving cognitions (i.e., panic‐related cognitions, accident‐related cognitions, and social‐related cognitions), rumination, and posttraumatic stress disorder (PTSD) symptoms in a sample of road traffic accidents (RTAs) survivors. We also investigated the indirect effect of driving cognitions on PTSD symptoms through rumination. The sample included 633 drivers (62% were men; Mage = 36.09; standard deviation [SD] = 11.42 years). The participants completed scales measuring driving cognitions, rumination, and PTSD symptoms, as well as providing their demographic information. The results showed that all three types of driving cognitions assessed in the present study and rumination were significantly positively associated with PTSD symptoms. Furthermore, rumination mediated the relation between social‐related cognitions and PTSD symptoms. The implications for PTSD treatment and future research are discussed.
Patient‐Focused Drug Development Methods for Benefit–Risk Assessments: A Case Study Using a Discrete Choice Experiment for Antiepileptic Drugs
Authors: Holmes Emily A.F. , Plumpton Catrin , Baker Gus A. , Jacoby Ann , Ring Adele , Williamson Paula , Marson Anthony , Hughes Dyfrig A. 
Published: September 2018

Regulatory decisions may be enhanced by incorporating patient preferences for drug benefit and harms. This study demonstrates a method of weighting clinical evidence by patients’ benefit–risk preferences. Preference weights, derived from discrete choice experiments, were applied to clinical trial data to estimate the expected utility of alternative drugs. In a case study, the rank ordering of antiepileptic drugs (AEDs), as indicated from clinical studies, was compared with ordering based on weighting clinical evidence by patients’ preferences. A statistically significant change in rank ordering of AEDs was observed for women of childbearing potential who were prescribed monotherapy for generalized or unclassified epilepsy. Rank ordering inferred from trial data, valproate > topiramate > lamotrigine, was reversed. Modeling the expected utility of drugs might address the need to use more systematic, methodologically sound approaches to collect patient input that can further inform regulatory decision making.
Advances in mass spectrometry‐based glycoproteomics
Authors: Yu Aiying , Zhao Jingfu , Peng Wenjing , Banazadeh Alireza , Williamson Seth D. , Goli Mona , Huang Yifan , Mechref Yehia 
Published: September 2018

AbstractProtein glycosylation, an important PTM, plays an essential role in a wide range of biological processes such as immune response, intercellular signaling, inflammation, and host–pathogen interaction. Aberrant glycosylation has been correlated with various diseases. However, studying protein glycosylation remains challenging because of low abundance, microheterogeneities of glycosylation sites, and poor ionization efficiency of glycopeptides. Therefore, the development of sensitive and accurate approaches to characterize protein glycosylation is crucial. The identification and characterization of protein glycosylation by MS is referred to as the field of glycoproteomics. Methods such as enrichment, metabolic labeling, and derivatization of glycopeptides in conjunction with different MS techniques and bioinformatics tools, have been developed to achieve an unequivocal quantitative and qualitative characterization of glycoproteins. This review summarizes the recent developments in the field of glycoproteomics over the past 6 years (2012 to 2018).
Effect of trace chloride on the char formation and flame retardancy of the LLDPE filled with NiAl‐layered double hydroxides
Authors: Wang Hongchao , Tan Shengnan , Song Rongjun 
Published: September 2018

SummaryLayered double hydroxides (LDHs) have received intensive attentions for the potential as flame retardants of polyolefin. In the work, trace amounts of chloride were investigated for their synergistic effects on the char formation and flame retardancy of the linear low‐density polyethylene (LLDPE) filled with NiAl‐LDHs. Results showed that 0.5 wt% of NH4Cl incorporation enabled the char yield of 20%LDH/LLDPE (20 wt% of NiAl‐LDH) increase from 10.4% to 49.6%. Other chlorides likewise offered significant increase in the char yield of 20%LDH/LLDPE. With the flame‐retardant measurements of cone calorimeter and limiting oxygen index device, it is revealed that the flame retardancy of LLDPE filled with NiAl‐LDHs could be greatly improved when trace amounts of NH4Cl were further introduced. Thermal stability analysis illustrated that the presence of NiAl‐LDHs or NH4Cl all had positive effects on the thermal stability of LLDPE, in which the chlorides influenced the LLDPE thermal stability via direct participation in the degradation of LLDPE. The synergetic mechanism analysis reveals that the introduction of chloride enabled the LLDPE decomposed products have more tendency to grow carbon nanotubes with the presence of NiAl‐LDH catalysts. Finally, the mechanical properties of LLDPE filled with NiAl‐LDHs and NH4Cl were also investigated.
Point forecasting of intraday volume using Bayesian autoregressive conditional volume models
Authors: Huptas Roman 
Published: September 2018

AbstractIn this paper, we apply Bayesian inference to model and forecast intraday trading volume, using autoregressive conditional volume (ACV) models, and we evaluate the quality of volume point forecasts. In the empirical application, we focus on the analysis of both in‐ and out‐of‐sample performance of Bayesian ACV models estimated for 2‐minute trading volume data for stocks quoted on the Warsaw Stock Exchange in Poland. We calculate two types of point forecasts, using either expected values or medians of predictive distributions. We conclude that, in general, all considered models generate significantly biased forecasts. We also observe that the considered models significantly outperform such benchmarks as the naïve or rolling means forecasts. Moreover, in terms of root mean squared forecast errors, point predictions obtained within the ACV model with exponential distribution emerge superior compared to those calculated in structures with more general innovation distributions, although in many cases this characteristic turns out to be statistically insignificant. On the other hand, when comparing mean absolute forecast errors, the median forecasts obtained within the ACV models with Burr and generalized gamma distribution are found to be statistically better than other forecasts.
Mutational and cytogenetic analyses of 188 CLL patients with trisomy 12: A retrospective study from the French Innovative Leukemia Organization (FILO) working group
Authors: Roos‐Weil Damien , Nguyen‐Khac Florence , Chevret Sylvie , Touzeau Cyrille , Roux Clémence , Lejeune Julie , Cosson Adrien , Mathis Stéphanie , Feugier Pierre , Leprêtre Stéphane , Béné Marie‐Christine , Baron Marine , Raynaud Sophie , Struski Stéphanie , Eclache Virginie , Sutton Laurent , Lesty Claude , Merle‐Béral Hélène , Cymbalista Florence , Ysebaert Loïc , Davi Frédéric , Leblond Véronique ,   on behalf of the FILO working group
Published: September 2018

AbstractTrisomy 12 (tri12) is the second most frequent chromosomal aberration (15%‐20%) in chronic lymphocytic leukemia (CLL). Tri12 confers an intermediate prognosis but is a heterogeneous entity. We examined whether additional mutational or chromosomal alterations might impact tri12 patient outcomes. This retrospective study, carried out by the French Innovative Leukemia Organization, included 188 tri12 patients with comprehensive information on immunoglobulin heavy chain (IGHV) gene status, karyotypic/FISH abnormalities, and NOTCH1, TP53, SF3B1, and MYD88 mutations. The main cytogenetic abnormalities associated with tri12 were del(13q) (25%), additional trisomies (14%) (including tri19 (10%) and tri18 (4%)), 14q32 translocations (10%), del(17p) (6.5%), del(14q) (4%), and del(11q) (4%). Unmutated (UM) IGHV, NOTCH1, and TP53, mutations were identified in respectively 66%, 25%, and 8.5% of cases. Multivariate analyses showed that additional trisomies (HR = 0.43, 95% CI = 0.23‐0.78, P = .01) were associated with a significantly longer time to first treatment in Binet stage A patients and with a lower risk of relapse (HR = 0.37, 95% CI = 0.15‐0.9, P = .03) in the overall tri12 population. Binet stage B/C, TP53 disruption, and UM IGHV status were associated with a shorter time to next treatment, while Binet stage B/C (HR = 4, 95% CI = 1.6‐4.9, P = .002) and TP53 disruption (HR = 5, 95% CI = 1.94‐12.66, P = .001) conferred shorter overall survival in multivariate comparisons. These data indicate that additional cytogenetic and mutational abnormalities, and particularly additional trisomies, IGHV status, and TP53 disruption, influence tri12 patient outcomes and could improve risk stratification in this population.
The influence of marital status on the survival of patients with operable gastrointestinal stromal tumor: A SEER‐based study
Authors: Chen Mo , Wang Xuan , Wei Ran , Wang Zheng 
Published: September 2018

SummaryBackgroundMarital status has been demonstrated as a predictive factor for prognosis in diverse malignancies, but its role in the survival of gastrointestinal stromal tumor (GIST) remains unclear.Material and methodsThis study was aimed to investigate the impact of marital status on the overall survival (OS) and cancer‐specific survival (CSS) of operable GIST cases, by using data from Surveillance, Epidemiology, and End Results (SEER) database. In total, 6582 GIST patients between 1973 and 2013 were identified from SEER database.ResultsUnivariate and multivariate analyses demonstrated the marital status as an independent predictive factor for survival. Married group had better 5‐year OS and CSS than unmarried group (OS: 79.2% vs 71.3%, P < .001; CSS: 88.9% vs 84.8%, P < .001). And compared to married, divorced/separated, and single group, widowed group had the lowest 5‐year OS and CSS (61.3% and 82.2%). Further subgroup analyses validated the relationship between marital status and survival among all SEER stages and different age groups. And additional sensitivity analyses according to other clinicopathological features confirmed that married cases had higher survival rate than unmarried cases.ConclusionIn conclusion, marriage could be a protective prognostic factor for survival, and widowed patients had a higher risk of death.
Remote ischemic conditioning as a cytoprotective strategy in vasculopathies during hyperhomocysteinemia: An emerging research perspective
Authors: Majumder Avisek , Singh Mahavir , George Akash K. , Homme Rubens Petit , Laha Anwesha , Tyagi Suresh C. 
Published: September 2018

AbstractHigher levels of nonprotein amino acid homocysteine (Hcy), that is, hyperhomocysteinemia (HHcy) (~5% of general population) has been associated with severe vasculopathies in different organs; however, precise molecular mechanism(s) as to how HHcy plays havoc with body’s vascular networks are largely unknown. Interventional modalities have not proven beneficial to counter multifactorial HHcy’s effects on the vascular system. An ancient Indian form of exercise called ‘yoga’ causes transient ischemia as a result of various body postures however the cellular mechanisms are not clear. We discuss a novel perspective wherein we argue that application of remote ischemic conditioning (RIC) could, in fact, deliver anticipated results to patients who are suffering from chronic vascular dysfunction due to HHcy. RIC is the mechanistic phenomenon whereby brief episodes of ischemia‐reperfusion events are applied to distant tissues/organs; that could potentially offer a powerful tool in mitigating chronic lethal ischemia in target organs during HHcy condition via simultaneous reduction of inflammation, oxidative and endoplasmic reticulum stress, extracellular matrix remodeling, fibrosis, and angiogenesis. We opine that during ischemic conditioning our organs cross talk by releasing cellular messengers in the form of exosomes containing messenger RNAs, circular RNAs, anti‐pyroptotic factors, protective cytokines like musclin, transcription factors, small molecules, anti‐inflammatory, antiapoptotic factors, antioxidants, and vasoactive gases. All these could help mobilize the bone marrow–derived stem cells (having tissue healing properties) to target organs. In that context, we argue that RIC could certainly play a savior’s role in an unfortunate ischemic or adverse event in people who have higher levels of the circulating Hcy in their systems.
Identification of novel biomarkers for hepatocellular carcinoma using transcriptome analysis
Authors: Xia Qianlin , Li Zehuan , Zheng Jianghua , Zhang Xu , Di Yang , Ding Jin , Yu Die , Yan Li , Shen Longqiang , Yan Dong , Jia Ning , Chen Weiping , Feng Yanling , Wang Jin 
Published: September 2018

AbstractHepatocellular carcinoma (HCC) is the third leading cause of death from cancer in the world. To comprehensively investigate the utility of microRNAs (miRNAs) and protein‐encoding transcripts (messenger RNAs [mRNAs]) in HCC as potential biomarkers for early detection and diagnosis, we exhaustively mined genomic data from three available omics datasets (GEO, Oncomine, and TCGA), analyzed the overlaps among gene expression studies from 920 hepatocellular carcinoma samples and 508 healthy (or adjacent normal) liver tissue samples available from six laboratories, and identified 178 differentially expressed genes (DEGs) associated with HCC. Paired with miRNA and lncRNA data, we identified 23 core genes that were targeted by nine differentially expressed miRNAs and 21 HCC‐specific lncRNAs. We further demonstrated that alterations in these 23 genes were quite frequent, with five genes altered in over 5% of the population. Patients with high levels of YWHAZ, ENAH, and HMGN4 tended to have high‐grade tumors and shorter overall survival, suggesting that these genes could be promising candidate biomarkers for disease and poor prognosis in patients with HCC. Our comprehensive mRNA, miRNA, and lncRNA omics analyses from multiple independent datasets identified robust molecules that may be used as biomarkers for early HCC detection and diagnosis.
NEAT1 induces osteosarcoma development by modulating the miR‐339‐5p/TGF‐β1 pathway
Authors: Zhang Lin , Lu Xiao‐Qing , Zhou Xiao‐Qing , Liu Qin‐Bai , Chen Liang , Cai Feng 
Published: September 2018

AbstractThe long noncoding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) has been recognized as a tumor oncogene involved in the development of multiple cancers. However, the function of NEAT1 and its molecular mechanism in osteosarcoma (OS) remain unclear. First, we detected the NEAT1 expression in OS cell lines by performing quantitative reverse‐transcription polymerase chain reaction. Next, the effects of NEAT1 on OS cell growth, apoptosis, migration, and invasion were tested by lentivirus‐mediated downregulation. We observed that inhibition of NEAT1 restrained OS cell progression greatly. Interestingly, in the last few years, increasing studies have shown that some lncRNAs can act as miRNA sponges and reduce the amount of the same. Here, we found that NEAT1 can modulate OS development via sponging miR‐339‐5p. MiR‐339‐5p was significantly decreased in OS cells, and its overexpression can remarkably repress the OS proliferation. These results indicated that NEAT1 could function as a tumor oncogene in OS by inhibiting miR‐339‐5p in vitro. Then, the following assays validated that transforming growth factor β1 (TGF‐β1) can act as a functional target of miR‐339‐5p in OS cells. Finally, we indicated that NEAT1 could mediate TGF‐β1 expression by competitively sponging miR‐339‐5p. NEAT1 induced OS cell proliferation and cell mobility by binding to miR‐339‐5p and increasing TGF‐β1 in OS. It was demonstrated in our study that lncRNA NEAT1 could impede miR‐339‐5p expression to maintain the expression of TGF‐β1, which led to the development of OS. Our findings implied that the novel identified NEAT1/miR‐339‐5p/TGF‐β1 axis might be a new molecular pathway or therapeutic target for OS diagnosis and treatment.
Tyrosine phosphorylation signaling regulates Ca2+ entry by affecting intracellular pH during human sperm capacitation
Authors: Brukman Nicolás Gastón , Nuñez Sol Yanel , Puga Molina Lis del Carmen , Buffone Mariano Gabriel , Darszon Alberto , Cuasnicu Patricia Sara , Da Ros Vanina Gabriela 
Published: September 2018

AbstractCapacitation is a mandatory process for the acquisition of mammalian sperm fertilization competence and involves the activation of a complex and still not fully understood system of signaling pathways. Under in vitro conditions, there is an increase in both protein tyrosine phosphorylation (pTyr) and intracellular Ca2+ levels in several species. In human sperm, results from our group revealed that pTyr signaling can be blocked by inhibiting proline‐rich tyrosine kinase 2 (PYK2). Based on the role of PYK2 in other cell types, we investigated whether the PYK2‐dependent pTyr cascade serves as a sensor for Ca
2+ signaling during human sperm capacitation. Flow cytometry studies showed that exposure of sperm to the PYK2 inhibitor
N‐[2‐[[[2‐[(2,3‐dihydro‐2‐oxo‐1
H‐indol‐5‐yl)amino]‐5‐(trifluoromethyl)‐4‐pyrimidinyl]amino]methyl]phenyl]‐
N‐methyl‐methanesulfonamide hydrate (PF431396) produced a significant and concentration‐dependent reduction in intracellular Ca
2+ levels during capacitation. Further studies revealed that PF431396‐treated sperm exhibited a decrease in the activity of CatSper, a key sperm Ca
2+ channel. In addition, time course studies during capacitation in the presence of PF431396 showed a significant and sustained decrease in both intracellular Ca
2+ and pH levels after 2 hr of incubation, temporarily coincident with the activation of PYK2 during capacitation. Interestingly, decreases in Ca
2+ levels and progressive motility caused by PF431396 were reverted by inducing intracellular alkalinization with NH
4Cl, without affecting the pTyr blockage. Altogether, these observations support pTyr as an intracellular sensor for Ca
2+ entry in human sperm through regulation of cytoplasmic pH. These results contribute to a better understanding of the modulation of the polymodal CatSper and signaling pathways involved in human sperm capacitation.
Preparation and evaluation of a polymer–metal–enzyme hybrid nanowire for the immobilization of multiple oxidoreductases
Authors: Zhang Yong‐Hui , Chen Kai‐Nan , Zhang Jun‐Nan , Ren Hong , Zhang Xin‐Yu , Huang Jing‐Han , Wang Shi‐Zhen , Fang Bai‐Shan 
Published: September 2018

AbstractBACKGROUNDThe immobilization of multiple oxidoreductases has attracted a lot of research interest for its industrial application in eco‐friendly production. Here, a novel immobilization strategy was developed using manganese ion chelated linear polyethyleneimine as artificial hybrid scaffold that allowed self‐assembly of bifunctional oxidoreductase (glycerol dehydrogenase‐NADH oxidase) via multipoint coordination interaction.RESULTThe immobilization formed a highly ordered polymer–metal–enzyme complex structure shaped like a leaf vein with 200‐nm‐wide nanowires. Preliminary results showed that the immobilization could not only retain but substantially increased the biological activity of the immobilized enzyme by 8.2‐fold. The equilibrium concentration of the reaction product DHA also was increased by 4.5‐fold compared with that of the free enzyme, exhibiting higher elimination efficiency of product inhibition compared with other enzyme immobilization strategy using nanoparticles or activated agarose beads. At the same time, linear polyethyleneimine (LPEI)–manganese scaffolds could prevent enzyme subunit disassociation and stabilize the active multimeric form, which improved catalytic condition adaption and thermal stability of target enzyme.CONCLUSIONThese results indicated a great potential of polymer–metal–enzyme hybrid in the enzyme immobilization and have provided a simple and efficient strategy for the immobilization of multiple oxidoreductases. © 2018 Society of Chemical Industry