bluuebunny/crossref_metadata_2025_split huggingface dataset:
Testing the light:nutrient hypothesis: Insights into biofilm structure and function using metatranscriptomics
Authors: Veach Allison M. , Griffiths Natalie A. 
Published: July 2018

AbstractAquatic biofilms are hotspots of biogeochemical activity due to concentrated microbial biomass (Battin, Kaplan, Newbold, & Hansen, ). However, biofilms are often considered a single entity when their role in biogeochemical transformations is assessed, even though these biofilms harbour functionally diverse microbial communities (Battin, Besemer, Bengtsson, Romani, & Packmann, ; Veach, Stegen, Brown, Dodds, & Jumpponen, ). Often overlooked are the biotic interactions among biofilm components that can affect ecosystem‐scale processes such as primary production and nutrient cycling. These interactions are likely to be especially important under resource limitation. Light is a primary resource mediating algal photosynthesis and both phototrophic and heterotrophic production due to bacterial reliance on C‐rich algal exudates (Cole, ). However, current understanding of function–structure linkages in streams has yet to unravel the relative degree of these microbial feedbacks under resource availability gradients. In this issue of Molecular Ecology, Bengtsson, Wagner, Schwab, Urich, and Battin () studied stream biofilm responses to light availability to understand its impact across three domains of life. By integrating biogeochemical rate estimation and metatranscriptomics within a microcosm experiment, they were able to link primary production and nutrient uptake rates to algal and bacterial metabolic processes and specify what taxa contributed to gene expression. Under low light, diatoms and cyanobacteria upregulated photosynthetic machinery and diatom‐specific chloroplast rRNA suggesting heightened transcriptional activity under light limitation to maintain phototrophic energy demands. Under high light, heterotrophic bacteria upregulated mRNAs related to phosphorous (P) metabolism while biofilm P uptake increased indicating high bacterial‐specific P demand when algal biomass was high. Together, these results indicate that biogeochemical function is mediated by complex microbial interactions across trophic levels.
High School Spanish Students and Foreign Language Reading Anxiety: Déjà vu All Over Again All Over Again
Authors: SPARKS RICHARD L. , LUEBBERS JULIE , CASTAÑEDA MARTHA , PATTON JON 
Published: July 2018

AbstractA unique anxiety for foreign language (L2) learning has been hypothesized to explain students’ problems with language learning. However, L2 anxiety instruments have been challenged on the grounds that they reflect students’ language learning ability and/or perceptions of their language learning skills. In this study, 266 U.S. high school students were administered measures of first language (L1) skills, L2 aptitude, and L2 achievement and followed through 2–3 years of Spanish courses. In 1st‐year Spanish, they were administered the Foreign Language Reading Anxiety Scale (FLRAS), divided into 3 anxiety groups, and compared on the L1 and L2 measures. Findings showed that the Low Anxiety group scored significantly higher than the High Anxiety group on all L1 and L2 measures, and significantly higher than the Average Anxiety group on most L1 and L2 measures at the end of 1st‐ and 2nd‐year Spanish. Students who completed 3rd‐year Spanish displayed either low or average anxiety on the FLRAS. Results revealed negative correlations between the FLRAS and all L1 and L2 measures. Correlations between the FLRAS and the L2 achievement measures increased over time. Findings show that the FLRAS is unlikely to be measuring anxiety for language learning but more likely to be a proxy for students’ levels of L1 skill, L2 aptitude, and L2 achievement.
Plant carbon metabolism and climate change: elevated CO2 and temperature impacts on photosynthesis, photorespiration and respiration
Authors: Dusenge Mirindi Eric , Duarte André Galvao , Way Danielle A. 
Published: July 2018

Contents

Summary
32

I.
The importance of plant carbon metabolism for climate change
32

II.
Rising atmospheric CO2 and carbon metabolism
33

III.
Rising temperatures and carbon metabolism
37

IV.
Thermal acclimation responses of carbon metabolic processes can be best understood when studied together
38

V.
Will elevated CO2 offset warming‐induced changes in carbon metabolism?
40

VI.
No plant is an island: water and nutrient limitations define plant responses to climate drivers
41

VII.
Conclusions
42

Acknowledgements
42

References
42

Appendix A1
48

SummaryPlant carbon metabolism is impacted by rising CO2 concentrations and temperatures, but also feeds back onto the climate system to help determine the trajectory of future climate change. Here we review how photosynthesis, photorespiration and respiration are affected by increasing atmospheric CO2 concentrations and climate warming, both separately and in combination. We also compile data from the literature on plants grown at multiple temperatures, focusing on net CO2 assimilation rates and leaf dark respiration rates measured at the growth temperature (Agrowth and Rgrowth, respectively). Our analyses show that the ratio of Agrowth to Rgrowth is generally homeostatic across a wide range of species and growth temperatures, and that species that have reduced Agrowth at higher growth temperatures also tend to have reduced Rgrowth, while species that show stimulations in Agrowth under warming tend to have higher Rgrowth in the hotter environment. These results highlight the need to study these physiological processes together to better predict how vegetation carbon metabolism will respond to climate change.
A stomatal control model based on optimization of carbon gain versus hydraulic risk predicts aspen sapling responses to drought
Authors: Venturas Martin D. , Sperry John S. , Love David M. , Frehner Ethan H. , Allred Michael G. , Wang Yujie , Anderegg William R. L. 
Published: July 2018

Summary

Empirical models of plant drought responses rely on parameters that are difficult to specify a priori. We test a trait‐ and process‐based model to predict environmental responses from an optimization of carbon gain vs hydraulic risk.

We applied four drought treatments to aspen (Populus tremuloides) saplings in a research garden. First we tested the optimization algorithm by using predawn xylem pressure as an input. We then tested the full model which calculates root‐zone water budget and xylem pressure hourly throughout the growing season.

The optimization algorithm performed well when run from measured predawn pressures. The per cent mean absolute error (MAE) averaged 27.7% for midday xylem pressure, transpiration, net assimilation, leaf temperature, sapflow, diffusive conductance and soil‐canopy hydraulic conductance. Average MAE was 31.2% for the same observations when the full model was run from irrigation and rain data. Saplings that died were projected to exceed 85% loss in soil‐canopy hydraulic conductance, whereas surviving plants never reached this threshold.

The model fit was equivalent to that of an empirical model, but with the advantage that all inputs are specific traits. Prediction is empowered because knowing these traits allows knowing the response to climatic stress.
Autophagy and its role in plant abiotic stress management
Authors: Avin‐Wittenberg Tamar 
Published: July 2018

AbstractBeing unable to move, plants are regularly exposed to changing environmental conditions, among which various types of abiotic stress, such as heat, drought, salt, and so forth. These might have deleterious effects on plant performance and yield. Plants thus need to adapt using appropriate stress responses. One of the outcomes of abiotic stress is the need to degrade and recycle damaged proteins and organelles. Autophagy is a conserved eukaryotic mechanism functioning in the degradation of proteins, protein aggregates, and whole organelles. It was previously shown to have a role in plant abiotic stress. This review will describe the current knowledge regarding the involvement of autophagy in plant abiotic stress response, mechanisms functioning in autophagy induction during stress, and possible direction for future research.
Function as Use. Wittgenstein's Practical Turn in the Early Manuscripts
Authors: Franken Figueiredo Florian 
Published: July 2018

AbstractThe idea that the function of language is its use is commonly ascribed to the Later Wittgenstein. In this paper, I argue that there is textual evidence already coming from the early manuscripts (Ms) proving that Wittgenstein's philosophical development is culminating in the idea of function as use around 1929–30. I interpret a passage from Ms‐107 in order to show that Wittgenstein's practical turn has sources in different stages of his philosophical development, each of which is dominated by different ideas: the idea of a picture theory; the idea of a phenomenological language and the idea of function as use.
Political Embeddedness of Public Pension Governance: An Event History Analysis of Discount Rate Changes
Authors: Wang Qiushi , Peng Jun 
Published: July 2018

AbstractState governments are frequently said to manipulate the discount rate assumption to make pension funding look better, reduce employers' and employees' pension contributions, or relieve fiscal stress. Building a model from the political embeddedness perspective and applying an event history analysis to the 81 largest state‐administered pension plans in the United States, the authors found that more politically embedded pension boards were actually more likely to reduce their plan's discount rate. Public union coverage and government political ideology, however, had no significant impact on discount rate changes. These findings reveal the effect of political embeddedness on pension planning decisions and provide useful insights into the intricate process of setting pension discount rates in a new era of more muted investment return expectations. This article points to both political and financial pressures facing pension boards and state governments for many years to come.
Phylogeny and evolution of the cryptic fungus‐farming ant genusMyrmicocryptaF. Smith (Hymenoptera: Formicidae) inferred from multilocus data
Authors: Sosa‐Calvo Jeffrey , Fernández Fernando , Schultz Ted R. 
Published: July 2018

AbstractFungus‐farming ants (Hymenoptera: Formicidae) have become model systems for exploring questions regarding the evolution of symbiosis. However, robust phylogenetic studies of both the ant agriculturalists and their fungal cultivars are necessary for addressing whether or not observed ant–fungus associations are the result of coevolution and, if so, whether that coevolution has been strict or diffuse. Here we focus on the evolutionary relationships of the species within the ant genusMyrmicocryptaand of their fungal cultivars. The fungus‐farming ant genusMyrmicocryptawas created by Fr. Smith in 1860 based on a single alate queen. Since then, 31 species and subspecies have been described. Until now, the genus has not received any taxonomic treatment and the relationships of the species within the genus have not been tested. Our molecular analyses, using ∼40 putative species and six protein‐coding (nuclear and mitochondrial) gene fragments, recoverMyrmicocryptaas monophyletic and as the sister group of the genusMycocepurusForel. The speciesM. tuberculataWeber is recovered as the sister to the rest ofMyrmicocrypta. The time‐calibrated phylogeny recovers the age of stem groupMyrmicocryptaplus its sister group as 45 Ma, whereas the inferred age for the crown groupMyrmicocryptais recovered as 27 Ma. Ancestral character‐state analyses suggest that the ancestor ofMyrmicocryptahad scale‐like or squamate hairs and that, although such hairs were once considered diagnostic for the genus, the alternative state of erect simple hairs has evolved at least seven independent times. Ancestral‐state analyses of observed fungal cultivar associations suggest that the most recent common ancestor ofMyrmicocryptacultivated clade 2 fungal species and that switches to clade 1 fungi have occurred at least five times. It is our hope that these results will encourage additional species‐level phylogenies of fungus‐farming ants and their fungal cultivars, which are necessary for understanding the evolutionary processes that gave rise to agriculture in ants and that produced the current diversity of mutualistic ant–fungus interactions.
Subdiffraction‐resolution live‐cell imaging for visualizing thylakoid membranes
Authors: Iwai Masakazu , Roth Melissa S. , Niyogi Krishna K. 
Published: July 2018

SummaryThe chloroplast is the chlorophyll‐containing organelle that produces energy through photosynthesis. Within the chloroplast is an intricate network of thylakoid membranes containing photosynthetic membrane proteins that mediate electron transport and generate chemical energy. Historically, electron microscopy (EM) has been a powerful tool for visualizing the macromolecular structure and organization of thylakoid membranes. However, an understanding of thylakoid membrane dynamics remains elusive because EM requires fixation and sectioning. To improve our knowledge of thylakoid membrane dynamics we need to consider at least two issues: (i) the live‐cell imaging conditions needed to visualize active processes in vivo; and (ii) the spatial resolution required to differentiate the characteristics of thylakoid membranes. Here, we utilize three‐dimensional structured illumination microscopy (3D‐SIM) to explore the optimal imaging conditions for investigating the dynamics of thylakoid membranes in living plant and algal cells. We show that 3D‐SIM is capable of examining broad characteristics of thylakoid structures in chloroplasts of the vascular plant Arabidopsis thaliana and distinguishing the structural differences between wild‐type and mutant strains. Using 3D‐SIM, we also visualize thylakoid organization in whole cells of the green alga Chlamydomonas reinhardtii. These data reveal that high light intensity changes thylakoid membrane structure in C. reinhardtii. Moreover, we observed the green alga Chromochloris zofingiensis and the moss Physcomitrella patens to show the applicability of 3D‐SIM. This study demonstrates that 3D‐SIM is a promising approach for studying the dynamics of thylakoid membranes in photoautotrophic organisms during photoacclimation processes.
Basic newborn resuscitation guidelines for healthcare providers in Maragua District Hospital: a best practice implementation project
Authors: Kebaya Lilian Maureen Nyatichi , Kiruja Jason , Maina Michuki , Kimani Salome , Kerubo Christine , McArthur Alexa , Munn Zachary , Ayieko Philip 
Published: July 2018

ABSTRACT

Objective:
The overall aim of this project was to assess compliance with evidence-based criteria regarding newborn resuscitation among healthcare workers in the maternity units in Maragua District Hospital (a busy district hospital in Kenya).

Introduction:
Globally, the majority of deaths under five years are attributable to neonatal causes. Perinatal asphyxia accounts for a significant portion of these deaths, especially in developing countries. These deaths are largely preventable, and interventions geared towards assisting newborns to initiate their first breath within the first minute of life have markedly reduced mortality.

Methods:
This implementation project was conducted in the maternity units of a busy district hospital. Evidence-based audit criteria were developed on the basis of an evidence summary developed by the Joanna Briggs Institute (JBI). Using the JBI Practical Application of Clinical Evidence System software (JBI PACES), a baseline audit was conducted including a sample size of 55 healthcare providers and 300 patient case notes followed by an identification of potential barriers and strategies to overcome them. A follow-up audit including a sample size of 55 healthcare providers was conducted by using the same audit criteria.

Results:
Improvements in practice were demonstrated in all criteria. The baseline audit demonstrated that three of the five audit criteria were found to be less than 50%, indicating moderate compliance with current evidence with regards to newborn resuscitation. Following implementation of the strategies, which included a six-week education and demonstrative skills training, and updating of the protocols and equipment, there was a significant improvement in all the criteria audited, with the first four criteria achieving 100% compliance, and the fifth criteria achieving 90% compliance.

Conclusion:
On completion of the project, the participants demonstrated an increase in knowledge and skills on newborn resuscitation, which led to a significant reduction in admission of newborns with birth asphyxia to the newborn unit.
Reef fish carbonate production assessments highlight regional variation in sedimentary significance
Authors: Salter Michael A. , Perry Chris T. , Stuart-Smith Rick D. , Edgar Graham J. , Wilson Rod W. , Harborne Alastair R. 
Published: July 2018

Abstract
Recent studies show that all marine bony fish produce mud-sized (&lt;63 µm) carbonate at rates relevant to carbonate sediment budgets, thus adding to the debate about the often enigmatic origins of fine-grained marine carbonates. However, existing production data are geographically and taxonomically limited, and because different fish families are now known to produce different carbonate polymorphs—an issue relevant to predicting their preservation potential—these limitations represent an important knowledge gap. Here we present new data from sites in the Western Pacific Ocean, based on an analysis of 45 fish species. Our data show that previously reported production outputs (in terms of rates and family-specific mineralogies) are applicable across different biogeographic regions. On this basis, we model carbonate production for nine coral reef systems around Australia, with production rates averaging 2.1–9.6 g m–2 yr–1, and up to 105 g m–2 yr–1 at discrete sites with high fish biomass. With projected production rates on lower-latitude reefs up to two-fold higher, these outputs indicate that carbonate production rates by fish can be comparable with other fine-grained carbonate-producing taxa such as codiacean algae. However, carbonates produced by Australian reef fish assemblages are dominated by a highly unstable amorphous polymorph; a marked contrast to Caribbean assemblages in which Mg calcite dominates. These findings highlight important regional differences in the sedimentary relevance and preservation potential of fish carbonates as a function of historical biogeographic processes that have shaped the world’s marine fish faunas.
Patient characteristics and risk factors for central serous chorioretinopathy: an analysis of 811 patients
Authors: Ersoz M Giray , Arf Serra , Hocaoglu Mumin , Sayman Muslubas Isil , Karacorlu Murat 
Published: July 2018

AimsTo determine the demographic and morphological characteristics of patients with central serous chorioretinopathy (CSC) and describe risk factors for CSC.MethodsWe retrospectively reviewed medical records of 811 patients with CSC and 816 healthy controls referred from 2002 to 2018.ResultsThe female/male ratio of patients with CSC was 1/2.7. Mean age of onset was 45.2 years. The peak prevalence for men occurred at 45–49 years. Women had two prevalence peaks, the higher at 55–59 years and the other at 45–49 years. Of these patients, 56.8% had unilateral disease, 42.1% had bilateral disease and 1.1% had only one eye. 671 (82.7%) patients had spectral domain optical coherence tomography (SD-OCT) images and 598 (73.7%) had enhanced depth choroidal images. Pigment epithelial detachment (PED) was detected in 763 (80.7%) of 945 eyes with SD-OCT images. Chronic cases were more likely to be bilateral and multifocal and to have PED (all p<0.001). Subfoveal choroidal thickness (SFCT) did not differ between chronic and classic cases (p=0.74), but SFCT was greater in multifocal cases than unifocal cases (p<0.001). In multivariate regression analyses, older age of onset, longer duration of disease and hyperopia were positively associated with having chronic disease rather than classic disease, and myopia and thyroid hormone replacement were negatively associated. Steroid use, antidepressant or anxiolytic drug use, smoking, pregnancy and hyperopia were risk factors, and myopia was a protective factor for CSC.ConclusionThis is the largest case-control study of CSC to evaluate demographic morphological characteristics and risk factors. Multiple factors are associated with CSC.
How do policymakers interpret and implement the principle of equivalence with regard to prison health? A qualitative study among key policymakers in England
Authors: Ismail Nasrul , de Viggiani Nick 
Published: July 2018

BackgroundThe principle of equivalence in prison health has been established for nearly four decades. It seeks to ensure that prisoners have access to the same level of healthcare as members of society at large, which is entrenched within the international legal framework and England’s national health policies.AimsThis study examined how key policymakers interpret and implement the principle of equivalence in English prisons. It also identified opportunities and threats associated with the application of the principle.MethodsIn total, 30 policymakers took part in this research. These participants engaged in policymaking activities and occupied positions of authority in the prison field.ResultsDespite the policymakers’ consensus on the importance of the equivalence principle, there was a varying degree of understanding regarding what constitutes ‘equivalence’. Participants described how the security culture impedes prisoners’ access to healthcare services. Additionally, the increasing size and complexity of the prison population, coupled with a diminishing level of resources, reduce the level of care being provided in prisons and thus compromise implementation of equivalence in English prisons.ConclusionsInconsistent interpretation of equivalence, the prevailing security drive, increasing numbers and health complexities of prisoners and fiscal austerity threaten the implementation of equivalence in English prisons. This research calls for new guidance on how to interpret and implement equivalence, along with measures to educate prison governors and staff on the prison rehabilitation value, ensure greater investment in prison health and consider alternatives to imprisonment to future-proof the principle of equivalence in the English prison system.
Reconstruction of Weakly Simple Polygons from Their Edges
Authors: Akitaya Hugo A. , Tóth Csaba D. 
Published: July 2018

We address the problem of reconstructing a polygon from the multiset of its edges. Given [Formula: see text] line segments in the plane, find a polygon with [Formula: see text] vertices whose edges are these segments, or report that none exists. It is easy to solve the problem in [Formula: see text] time if we seek an arbitrary polygon or a simple polygon. We show that the problem is NP-complete for weakly simple polygons, that is, a polygon whose vertices can be perturbed by at most [Formula: see text], for any [Formula: see text], to obtain a simple polygon. We give [Formula: see text]-time algorithms for reconstructing weakly simple polygons: when all segments are collinear or the segment endpoints are in general position. These results extend to the variant in which the segments are directed. We study related problems for the case that the union of the [Formula: see text] input segments is connected. (i) If each segment can be subdivided into several segments, find the minimum number of subdivision points to form a weakly simple polygon. (ii) If new line segments can be added, find the minimum total length of new segments that creates a weakly simple polygon. We give worst-case upper and lower bounds for both problems.