bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐range projections from sparse populations of GABAergic neurons in murine subplate
Authors: Boon Jacqueline , Clarke Emma , Kessaris Nicoletta , Goffinet André , Molnár Zoltán , Hoerder‐Suabedissen Anna 
Published: December 2018

AbstractThe murine subplate contains some of the earliest generated populations of neurons in the cerebral cortex, which play an important role in the maturation of cortical inhibition. Here we present multiple lines of evidence, that the subplate itself is only very sparsely populated with GABAergic neurons at postnatal day (P)8. We used three different transgenic mouse lines, each of which labels a subset of GABAergic, ganglionic eminence derived neurons. Dlx5/6‐eGFP labels the most neurons in cortex (on average 11% of NEUN+ cells across all layers at P8) whereas CGE‐derived Lhx6‐Cre::Dlx1‐Venusfl cells are the sparsest (2% of NEUN+ cells across all layers at P8). There is significant variability in the layer distribution of labeled interneurons, with Dlx5/6‐eGFP and Lhx6‐Cre::R26R‐YFP being expressed most abundantly in Layer 5, whereas CGE‐derived Lhx6‐Cre::Dlx1‐Venusfl cells are least abundant in that layer. All three lines label at most 3% of NEUN+ neurons in the subplate, in contrast to L5, in which up to 30% of neurons are GFP+ in Dlx5/6‐eGFP. We assessed all three GABAergic populations for expression of the subplate neuron marker connective tissue growth factor (CTGF). CTGF labels up to two‐thirds of NEUN+ cells in the subplate, but was never found to colocalize with labeled GABAergic neurons in any of the three transgenic strains. Despite the GABAergic neuronal population in the subplate being sparse, long‐distance axonal connection tracing with carbocyanine dyes revealed that some Gad65‐GFP+ subplate cells form long‐range axonal projections to the internal capsule or callosum.
What color do you feel? Color choices are driven by mood
Authors: Jonauskaite Domicele , Althaus Betty , Dael Nele , Dan‐Glauser Elise , Mohr Christine 
Published: December 2018

AbstractPopular opinion holds that color has specific affective meaning. Brighter, more chromatic, and warm colors were conceptually linked to positive stimuli and darker, less chromatic, and cool colors to negative stimuli. Whether such systematic color associations exist with actually mood felt remains to be tested. We experimentally induced four moods—joy, relaxation, fear, and sadness—in a between‐subject design (N = 96). Subsequently, we asked participants to select a color, from an unrestricted sample, best representing their current mood. Color choices differed between moods on hue, lightness, and chroma. Yellow hues were systematically associated with joy while yellow‐green hues with relaxation. Lighter colors were matched to joy and relaxation (positive moods) than fear and sadness (negative moods). Most chromatic colors were matched to joy, then relaxation, fear, and sadness. We conclude that color choices represent felt mood to some extent, after accounting for a relatively low specificity for color‐mood associations.
Dental iron precipitates in patients with Type 2 diabetes
Authors: Ortiz‐Arrambide Miguel Angel , Juarez‐Ibarra Karla Isabel , Malagón‐Santiago Guadalupe Ismael , Cruz‐Fierro Norma , De La Garza‐Ramos Myriam Angelica 
Published: December 2018

AbstractDiabetes mellitus (DM) is a frequent worldwide disease. There are currently more than 46 million people who suffer this disease in North America and the Caribbean. The objective of this study was to determine if there is an association between DM and the presence of iron precipitates (Fe2+) in dental structure. The third molar was extracted for reasons that merit extraction from 40 individuals with and without DM to analyze dentin tissue. Horizontal and longitudinal slices of tooth samples were made and later stained with 10% potassium cyanoferrate. The samples were observed by optical microscope to identify basophilic elements. A nonparametric Spearman correlation was performed to find an association between the quantitative (gender, group, and dentinal tissue) and qualitative variables (gender). The Mann–Whitney U test was used to find differences in the means of the nonparametric variables in two different groups in relation to the P value (<0.05). Iron elements were found in the predentin and circumpulpal dentin areas, and the results obtained showed a statistically significant difference between dentin tissue from patients with diabetes and those without. Individuals with Type 2 DM are prone to present iron precipitates in predentin and circumpulpal dentin tissue. Few iron elements were found in dental organs of individuals without DM.
The role of BRCA1‐associated protein 1 in the diagnosis of malignant mesothelioma in effusion and fine‐needle aspiration cytology
Authors: Hatem Layla , McIntire Patrick J , He Bing , Gogineni Swarna , Ho Mai , Mathew Susan , Margolskee Elizabeth M. , Siddiqui Momin T. , Rao Rema A. 
Published: December 2018

BackgroundMalignant mesothelioma (MM) is a diagnostically challenging entity in cytology specimens due to the lack of architectural context and a cytomorphologic overlap between malignant and reactive mesothelial cells (RMCs). A diagnostic marker with excellent specificity is not currently available in clinical practice. The newly appreciated BRCA1‐associated protein 1 (BAP1) antibody may help distinguish MM from RMC based on its immunohistochemical (IHC) staining pattern but its role in cytopathology is controversial.MethodsImmunohistochemistry with BAP1 antibody was performed on cell blocks from 39 cytology specimens including 13 cases of RMC and 26 cases of effusion and fine‐needle aspiration specimens (FNAC) with tissue‐specimen‐proven MM. Cases were dichotomised into positive and negative cohorts. Positivity was defined as >50% loss of nuclear BAP1 IHC staining.ResultsOf the 26 MM cases, a slight majority (14/26, 54%) showed loss of BAP1 nuclear IHC staining, while all 13 RMC controls showed strong nuclear BAP1 IHC staining. MM was more likely to show loss of BAP1 than RMC (P < .001); and peritoneal MM was more likely to demonstrate loss of BAP1 than pleural MM (P = .04). There was perfect specificity at 1.0 and positive predictive value of 1.0 for loss of nuclear BAP1 IHC staining. However, only modest sensitivity at 0.52 and negative predictive value of 0.50 was seen.ConclusionThese data confirm that absence of BAP1 nuclear staining identifies malignant mesothelial cells. On the other hand, positive BAP1 nuclear staining can occur in both benign and malignant pleural effusions.
Intensive versus standard multifactorial cardiovascular risk factor control in screen‐detected type 2 diabetes: 5‐year and longer‐term modelled outcomes of the ADDITION‐Leicester study
Authors: Webb David , Dales Jolyon , Zaccardi Francesco , Hill Sian , Moore Corran , Farooqi Azhar , Griffin Simon , Davies Melanie , Khunti Kamlesh 
Published: December 2018

AbstractAimsDiabetes treatment algorithms recommend intensive intervention in those with a shorter duration of disease. Screening provides opportunities for earlier multifactorial cardiovascular risk factor control. Using data from the ADDITION‐Leicester study (NCT00318032), we estimated the effects of this approach on modelled risk of diabetes‐related complications in screen‐detected patients.MethodsA total of 345 (41% South Asian) people with screen‐detected type 2 diabetes were cluster randomised to receive 5 years of (1) intensive multifactorial risk factor intervention or (2) standard treatment according to national guidance. Estimated 10 to 20‐year risk of ischaemic heart disease, stroke, congestive cardiac failure, and death was calculated using UK‐PDS risk equations.ResultsCompared with standard care, mean treatment differences for intensive management at 5 years were −11.7(95%CI: −15.0, −8.4) and −6.6(−8.8, −4.4) mmHg for systolic and diastolic blood pressure, respectively; −0.27 (−0.66, −0.26) % for HbA1c; and −0.46(−0.66; −0.26), −0.34 (−0.51; −0.18), and −0.19 (−0.28; −0.10) mmol/L for total cholesterol, LDL‐cholesterol, and triglycerides, respectively. There was no significant weight gain in the intensive group despite additional medication use. Modelled risks were consistently lower for intensively managed patients. Absolute risk reduction associated with intensive treatment at 10 and 20 years were 3.5% and 6.2% for ischaemic heart disease and 6.3% and 8.8% for stroke. Risk reduction for congestive heart failure plateaued after 15 years at 5.3%. No differences were observed for blindness and all‐cause death.ConclusionIntensive multifactorial intervention in a multi‐ethnic population with screen‐detected type 2 diabetes results in sustained improvements in modelled ischaemic heart disease, stroke, and congestive cardiac failure.
Family‐based treatment: Where are we and where should we be going to improve recovery in child and adolescent eating disorders
Authors: Lock James , Le Grange Daniel 
Published: December 2018

AbstractObjectiveFamily therapy has long been advocated as an effective intervention for eating disorders. A specific form of family therapy, one that utilizes family resources, has proven especially effective for adolescents with anorexia or bulimia nervosa (AN and BN). First developed in London, a behaviorally focused adaptation, called family‐based treatment (FBT), has been manualized and systematically studied in six randomized clinical trials for adolescent AN and two for adolescent BN.MethodThis Commentary focuses on manualized FBT; what we know, what we do not know (yet), and what we hope for.ResultsWe do know that efficacy data for FBT, especially adolescent AN, are quite robust, even though remission rates remain elusive for more than half of all cases. While preliminary, moderators of FBT for adolescent AN have been identified and could aid us in determining the most (or least) responsive patient groups. And weight gain (∼2.5kg) by week four has been confirmed as an early predictor of remission at end‐of‐treatment. What we do not know, yet, is whether specific adaptations to manualized FBT will confer improved remission rates.DiscussionFinally, and in terms of what is hoped for, we highlight the promise of improved access, dissemination, and implementation of FBT.
An expanded molecular phylogeny of Plumbaginaceae, with emphasis on Limonium (sea lavenders): Taxonomic implications and biogeographic considerations
Authors: Koutroumpa Konstantina , Theodoridis Spyros , Warren Ben H. , Jiménez Ares , Celep Ferhat , Doğan Musa , Romeiras Maria M. , Santos‐Guerra Arnoldo , Fernández‐Palacios Jóse María , Caujapé‐Castells Juli , Moura Mónica , Menezes de Sequeira Miguel , Conti Elena 
Published: December 2018

AbstractPlumbaginaceae is characterized by a history of multiple taxonomic rearrangements and lacks a broad molecular phylogenetic framework. Limonium is the most species‐rich genus of the family with ca. 600 species and cosmopolitan distribution. Its center of diversity is the Mediterranean region, where ca. 70% of all Limonium species are endemic. In this study, we sample 201 Limonium species covering all described infrageneric entities and spanning its wide geographic range, along with 64 species of other Plumbaginaceae genera, representing 23 out of 29 genera of the family. Additionally, 20 species of the sister family Polygonaceae were used as outgroup. Sequences of three chloroplast (trnL‐F, matK, and rbcL) and one nuclear (ITS) loci were used to infer the molecular phylogeny employing maximum likelihood and Bayesian analyses. According to our results, within Plumbaginoideae, Plumbago forms a non‐monophyletic assemblage, with Plumbago europaea sister to Plumbagella, while the other Plumbago species form a clade sister to Dyerophytum. Within Limonioideae, Ikonnikovia is nested in Goniolimon, rejecting its former segregation as genus distinct from Goniolimon. Limonium is divided into two major clades: Limonium subg. Pteroclados s.l., including L. sect. Pteroclados and L. anthericoides, and L. subg. Limonium. The latter is divided into three well‐supported subclades: the monospecific L. sect. Limoniodendron sister to a clade comprising a mostly non‐Mediterranean subclade and a Mediterranean subclade. Our results set the foundation for taxonomic proposals on sections and subsections of Limonium, namely: (a) the newly described L. sect. Tenuiramosum, created to assign L. anthericoides at the sectional rank; (b) the more restricted circumscriptions of L. sect. Limonium (= L. sect. Limonium subsect. Genuinae) and L. sect. Sarcophyllum (for the Sudano‐Zambezian/Saharo‐Arabian clade); (c) the more expanded circumscription of L. sect. Nephrophyllum (including species of the L. bellidifolium complex); and (d) the new combinations for L. sect. Pruinosum and L. sect. Pteroclados subsect. Odontolepideae and subsect. Nobiles.
Factors of local recurrence and organ preservation with transoral laser microsurgery in laryngeal carcinomas; CHAID decision‐tree analysis
Authors: Vilaseca Isabel , Nogués‐Sabaté Anna , Avilés‐Jurado Francesc Xavier , Berenguer Joan , Grau Juan José , Verger Eugenia , Nadal Alfons , Muxí Africa , Bernal‐Sprekelsen Manuel , Blanch José Luis 
Published: December 2018

AbstractBackgroundIndications of transoral laser microsurgery (TLM) are conditioned by the risk of local relapse.ObjectiveTo evaluate prognostic factors of local relapse and local control with TLM (LC‐TLM).MethodsLocal relapse and LC‐TLM were evaluated in 1119 patients. Logistic regression and CHAID decision tree analysis were performed.ResultsLocal relapse correlated to previous radiotherapy failure (8.45, CI 95%: 2.64‐27.03; P < .001), paraglottic involvement (2.42, CI: 1.41‐4.15; P = .001), anterior commissure involvement (2.12, CI: 1.43‐3.14; P < .001), grade of differentiation (1.74, CI: 1.18‐2.57; P = .005), and alcohol consumption (1.4, CI: 0.99‐1.98; P = .057). Local relapse tended to inversely correlate with experience (0.73, CI: 0.51‐1.03; P = .078). The most important factors for local relapse were previous radiotherapy failure and anterior commissure involvement.LC‐TLM inversely correlated with previous radiotherapy failure (0.09, CI: 0.03‐0.28; P < .001), paraglottic involvement (0.25, CI: 0.14‐0.43; P < .001), anterior commissure involvement (0.49, CI: 0.32‐0.77; P = .007), margins (0.56, CI: 0.30‐1.04; P = .068), and differentiation (0.68, CI: 0.44‐1.05; P = .087). LC‐TLM correlated with experience (1.71, CI: 1.13‐2.55; P = .010). The most important factors for LC‐TLM were previous radiotherapy failure and paraglottic involvement.ConclusionPrevious radiotherapy failure is the most important factor for local relapse and LC‐TLM. In primary treatments, anterior commissure involvement and paraglottic involvement are the most important factors for local relapse and LC‐TLM, respectively.
Effect of sex hormone–binding globulin polymorphisms on the outcome of in vitro fertilization‐embryo transfer for polycystic ovary syndrome patients: A case‐control study
Authors: Liu Yang , Zhao Xi‐Xi , Hu Xiang‐Juan , Yang Feng , Lin Ping , Cui Shi‐Chang , Zhao Wei , Cao Xiao‐Yun , Wang Yong‐Sheng 
Published: December 2018

AbstractPolycystic ovary syndrome (PCOS), known as a common endocrine disorder among females, plagues many PCOS patients. The current study aimed to explore the correlations of sex hormone–binding globulin (SHBG) polymorphisms with the outcome of in vitro fertilization‐embryo transfer (IVF‐ET) in PCOS patients. PCOS patients who underwent IVF‐ET and patients with non–PCOS‐related infertility were selected in the study. Correlations of SHBG rs6259 and rs727428 with the risk factors in PCOS were analyzed, followed by the evaluation of the effect of SHBG polymorphisms on the outcome of IVF‐ET in PCOS patients. At last, unconditional logistic regression analysis was performed to study the risk factors for IVF‐ET treatment outcome. Compared with SHBG rs6259 GG carriers, the incidence of PCOS was found to be elevated in SHBG rs6259 GA+AA carriers which indicated that the A allele was a risk factor for PCOS. Compared with SHBG rs6259 TT carriers, the number of retrieved oocytes and embryo as well as the fertility rate in SHBG rs6259 GA+AA carriers was found to be decreased, while the abortion rate, incidence of ovarian hyperstimulation syndrome, transplant rejection rate, estradiol, and testosterone in serum, as well as testosterone in follicular fluid were elevated. The luteal hormone, serum testosterone, and progesterone and GA+AA genotype of rs6259 were the risk factors for IVF‐ET treatment outcome. Taken together, the study showed that SHBG rs6259 polymorphisms might be correlated with the risk of PCOS and the outcome of IVF‐ET treatment.
Beginning an Engineer's Journey: A Narrative Examination of How, When, and Why Students Choose the Engineering Major
Authors: Cruz Joshua , Kellam Nadia 
Published: December 2018

AbstractBackgroundWhile recent engineering education research has focused on the pathways that students take as they transition into engineering programs in higher education, little has explicitly focused on developing an understanding of students as they select and transfer into engineering from other majors.PurposeThe purpose of this paper is to gain a better understanding of how engineering students enter the engineering field by comparing commonalities across their experiences. Given recent literature that describes student dissatisfaction toward, misinformation about, and attrition in engineering programs, we explore the lived experiences of students entering the engineering field, with a particular focus on students changing their majors to engineering.MethodsThis paper synthesizes the stories of 21 undergraduate engineering students from a southeastern research university, 15 of whom began their undergraduate program in other majors and transferred into engineering. We employ a unique narrative structuralizing scheme based on Campbell's hero's journey and use the metaphor of the beginning of the journey to understand student trajectories that locate students in engineering studies.ConclusionsWith this information, we can better understand student conceptions of the engineering field when they enter; who enters the engineering field and why; how students’ expectations are met or not in engineering programs; and what are the factors that ultimately contribute to first‐year retention in engineering programs. In general, students entering engineering tend to have a limited understanding of what is entailed in an engineering program and benefit from interactions with advisors, teachers, and peers in the field. Such interactions may help students to more clearly decide what aspects of engineering are appropriate for them to pursue and help them to persist as they begin coursework.
Assembly of a miRNA‐modified QCM sensor for miRNA recognition through response patterns
Authors: Li Xue , Ma Ding , Zheng Sheng‐Run , Fan Jun , Wang Tai , Dai Zong , Zou Xiao‐Yong , Teng Shao‐Hua , Zhang Wei‐Guang 
Published: December 2018

AbstractIn this paper, a miRNA‐based quartz crystal microbalance (QCM) biosensor was fabricated and used to the rapid and effective sensing of miRNA. The specific hybridization between probe miRNA and different selected miRNAs (miR‐27a, miR‐27b, and Let‐7a) cause a different interaction mode, thus display different frequency change and response patterns in the QCM sensor, which were used to detect miR‐27a and miR‐27b. The selective sensing of miR‐27a in mixed miRNA solution was also achieved. This miRNA‐based QCM biosensor has the advantages of real‐time, label‐free, and short cycle detection.
Posttraumatic Stress Disorder and Cancer Risk: A Nested Case‐Control Study
Authors: Cohn Elana , Lurie Ido , Yang Yu‐Xiao , Bilker Warren B. , Haynes Kevin , Mamtani Ronac , Shacham‐Shmueli Einat , Margalit Ofer , Boursi Ben 
Published: December 2018

AbstractData regarding cancer risk for individuals who were exposed to traumatic and stressful life events are conflicting. We sought to evaluate the association between posttraumatic stress disorder (PTSD) and the risk of the four most common solid tumors: lung, breast, prostate, and colorectal cancers. We conducted four nested case‐control studies using a large UK population‐based database. Cases were defined as individuals with any medical code for the specific malignancy. For every case, we used incidence‐density sampling to match four controls by age, sex, practice site, and both duration and calendar time of follow‐up. Exposure of interest was any diagnosis of PTSD prior to cancer diagnosis. The odds ratios (ORs) and 95% confidence intervals (CIs) for cancer risk associated with PTSD were estimated using multivariable conditional logistic regression and were adjusted for smoking status, obesity, and antidepressant use. The study population included four case groups according to cancer type. There were 19,143 cases with lung cancer (74,473 matched controls), 22,163 cases with colorectal cancer (86,538 matched controls), 31,352 cases with breast cancer (123,285 matched controls), and 27,212 cases with prostate cancer (105,940 matched controls). There was no statistically significant association between PTSD and cancer risk among any of the cancer types: lung, OR = 0.73, 95% CI [0.43, 1.23]; breast, OR = 0.73, 95% CI [0.52, 1.01]; prostate, OR = 1.24, 95% CI [0.87, 1.77]; and colorectal, OR = 1.05, 95% CI [0.68, 1.62]. Our findings indicated that participants in our study with PTSD were not at increased risk of lung, breast, prostate, and colorectal cancers.