bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Swiss nationwide survey shows that dual guidance is the preferred approach for peripheral nerve blocks
Authors: Luedi Markus M. , Upadek Vanessa , Vogt Andreas P. , Steinfeldt Thorsten , Eichenberger Urs , Sauter Axel R. 
Published: June 2019

AbstractUltrasound has significantly increased safety and effectiveness in regional anesthesia. However, little is known about its clinical use. We studied clinical approaches currently used by anesthesiologists, conducted a nationwide survey, and analyzed data collected in ordered logistic regression models. All active members of the Swiss Society for Anaesthesiology and Resuscitation (SSAR/SGAR) were asked to participate. Reported practice in nerve localization, safety, and techniques used for peripheral nerve blocks (PNB) were main outcome measures. Experience ranged from 3 to >30 years. The mean number of block techniques mastered was 11.5 ± 5.9. Standard monitoring was regularly used, whereas sterile coats were less frequently used by anesthesiologists who self-estimated a higher level of expertise in PNB (ordered logit coefficient −0.05, 95% CI −0.07 to −0.02, P < 0.001; pseudo r2 = 0.019; probability > Chi2 = 0.02). The more self-estimated expertise anesthesiologists had, the less likely they were to use nerve stimulation in combination with ultrasound (dual guidance) (ordered logit coefficient −0.31; 95% CI −0.85 to −0.03: P = 0.03; pseudo r2 = 0.007; probability > Chi2 = 0.05). The high share of reported standard monitoring meets the recommendations of the Helsinki Patient Safety Declaration. Dual guidance appears to be the preferred approach for safely localizing nerves for PNB in Switzerland.
Simultaneous assay for protease activities of hepatitis C virus and human immunodeficiency virus based on fluorescence detection
Authors: Kabashima Tsutomu , Tonooka Keiko , Takada Makoto , Kai Masaaki , Shibata Takayuki 
Published: June 2019

AbstractHepatitis C virus protease (HCV-PR) and human immunodeficiency virus protease (HIV-PR) are important for virus maturation, and thus can be used as potential target molecules for the development of antiviral drugs for the treatment of viral infections. In this study, a novel assay was developed to determine HCV-PR activity. This assay is based on a fluorogenic reaction, in which peptide fragments generated from an acetyl peptide substrate by HCV-PR can be selectively converted into a fluorescent derivative, and quantified by high-performance liquid chromatography (HPLC) with fluorescent detection. Herein, several acetyl-peptides can be used as substrates for HPLC. The application of this assay was further validated by simultaneous detection of HCV-PR and HIV-PR in a reaction mixture. The proposed method can differentiate the enzyme activities of HCV-PR and HIV-PR in a sample using their corresponding substrates. The results suggest that this assay can detect various proteases by employing set of substrate peptides under the same reaction conditions.
Certain polynomials and related topological indices for the series of benzenoid graphs
Authors: Nadeem Muhammad , Yousaf Awais , Alolaiyan Hanan , Razaq Abdul 
Published: June 2019

AbstractA topological index of a molecular structure is a numerical quantity that differentiates between a base molecular structure and its branching pattern and helps in understanding the physical, chemical and biological properties of molecular structures. In this article, we quantify four counting polynomials and their related topological indices for the series of a concealed non-Kekulean benzenoid graph. Moreover, we device a new method to calculate the PI and Sd indices with the help of Theta and Omega polynomials.
Suit-type Wearable Robot Powered by Shape-memory-alloy-based Fabric Muscle
Authors: Park Seong Jun , Park Cheol Hoon 
Published: June 2019

AbstractA suit-type wearable robot (STWR) is a new type of soft wearable robot (SWR) that can be worn easily anywhere and anytime to assist the muscular strength of wearers because it can be worn like normal clothes and is comfortable to wear even with no power supply. This paper proposes an STWR, in which a shape-memory-alloy-based fabric muscle (SFM) is used as the actuator. The STWR, which weighs less than 1 kg, has a simple structure, with the following components: SFMs, wire encoders for measuring the contraction length of the SFMs, and BOA that fix the actuators on the forearms. In this study, a position controller for the SFM using the wire encoder was developed, and a prototype STWR was fabricated using this position controller. Moreover, by putting the STWR on a mannequin, step-response experiments were performed in which the arms of the mannequin lifted barbells weighing 2 kg and 4 kg to a certain target position. A fast response of moving to the target position in less than 1 s was observed in all steps except for the initial heating step for the 2 kg barbell. The response speed of the SFM was noticeably slower for the 4 kg barbell compared to that for the 2 kg barbell; it moved to the target position in approximately 3 s in all the steps except for the initial heating step. The SFM-applied STWR could overcome the limitations of conventional robots in terms of weight and inconvenience, thereby demonstrating the application potential of STWRs.
Orbital angular momentum transformation of optical vortex with aluminum metasurfaces
Authors: Zhang Yuchao , Yang Xiaodong , Gao Jie 
Published: June 2019

AbstractThe orbital angular momentum (OAM) transformation of optical vortex is realized upon using aluminum metasurfaces with phase distributions derived from the caustic theory. The generated OAM transformation beam has the well-defined Bessel-like patterns with multiple designed topological charges from −1 to +2.5 including both the integer-order and fractional-order optical vortices along the propagation. The detailed OAM transformation process is observed in terms of the variations of both beam intensity and phase profiles. The dynamic distributions of OAM mode density in the transformation are further analyzed to illustrate the conservation of the total OAM. The demonstration of transforming OAM states arbitrarily for optical vortex beams will lead to many new applications in optical manipulation, quantum optics, and optical communication.
Complementary substrate specificity and distinct quaternary assembly of the Escherichia coli aerobic and anaerobic β-oxidation trifunctional enzyme complexes
Authors: Sah-Teli Shiv K. , Hynönen Mikko J. , Schmitz Werner , Geraets James A. , Seitsonen Jani , Pedersen Jan Skov , Butcher Sarah J. , Wierenga Rik K. , Venkatesan Rajaram 
Published: June 2019

Abstract
The trifunctional enzyme (TFE) catalyzes the last three steps of the fatty acid β-oxidation cycle. Two TFEs are present in Escherichia coli, EcTFE and anEcTFE. EcTFE is expressed only under aerobic conditions, whereas anEcTFE is expressed also under anaerobic conditions, with nitrate or fumarate as the ultimate electron acceptor. The anEcTFE subunits have higher sequence identity with the human mitochondrial TFE (HsTFE) than with the soluble EcTFE. Like HsTFE, here it is found that anEcTFE is a membrane-bound complex. Systematic enzyme kinetic studies show that anEcTFE has a preference for medium- and long-chain enoyl-CoAs, similar to HsTFE, whereas EcTFE prefers short chain enoyl-CoA substrates. The biophysical characterization of anEcTFE and EcTFE shows that EcTFE is heterotetrameric, whereas anEcTFE is purified as a complex of two heterotetrameric units, like HsTFE. The tetrameric assembly of anEcTFE resembles the HsTFE tetramer, although the arrangement of the two anEcTFE tetramers in the octamer is different from the HsTFE octamer. These studies demonstrate that EcTFE and anEcTFE have complementary substrate specificities, allowing for complete degradation of long-chain enoyl-CoAs under aerobic conditions. The new data agree with the notion that anEcTFE and HsTFE are evolutionary closely related, whereas EcTFE belongs to a separate subfamily.
Association of microRNAs genes polymorphisms with arthritis: a systematic review and meta-analysis
Authors: Xiao Yingqi , Liu Hui , Chen Li , Wang Yang , Yao Xiang , Jiang Xiaolian 
Published: June 2019

Abstract
Objective: To investigate whether microRNAs genes’ polymorphisms are associated with arthritis. Methods: The PubMed, Cochrane Library et al. were systematically searched to identify case–control studies, systematic reviews and meta-analyses. A meta-analysis was performed to calculate odds ratios (ORs), and confidence intervals (CIs) at 95% using fixed-effect model or random-effects model. Results: Twenty-two case–control studies involving 10489 participants fulfilled the inclusion criteria. MiR-146a rs2910164 (G/C) was not significantly associated with the risk of rheumatoid arthritis (RA) in any model. Significant associations were found between miR-146a rs2910164 (G/C) and the risk of psoriatic arthritis (PsA) in the heterozygous model and the dominant model. The heterozygous model showed a significant association between the miR-146a rs2910164 (G/C) polymorphism and ankylosing spondylitis (AS). And there was no significant association of miR-146a rs2910164 (G/C) with risk of juvenile rheumatoid arthritis (JRA) at any model. Additionally, there was a significant association of miR-499 rs3746444 (T/C) with risk of RA at two genetic models, and with a moderate heterogeneity. When subgroup analysis by ethnicity, significant associations were almost found between miR-499 rs3746444 (T/C) and the risk of RA in any model in Caucasian populations, and there is no heterogeneity. Conclusions: The association of miR-146a rs2910164 (G/C) with RA was not found. And there was a significant association between miR-146a rs2910164(G/C) and PsA or AS. MiR-499 rs3746444 (T/C) was associated with RA in Caucasian populations. These findings did not support the genetic association between miR-146a rs2910164 (G/C) and JRA susceptibility, as well as the association of miR-196a-2 rs11614913 (C/T), miR-146a rs2431697, miR-146a rs57095329, miR-149 rs22928323 with arthritis.
The pseudogene PTENP1 regulates smooth muscle cells as a competing endogenous RNA
Authors: Lai Yanxian , Li Jianyong , Zhong Lintao , He Xiang , Si Xiaoyun , Sun Yili , Chen Yanmei , Zhong Jiayuan , Hu Yinlan , Li Bing , Liao Wangjun , Liu Cheng , Liao Yulin , Xiu Jiancheng , Bin Jianping 
Published: June 2019

Abstract
The long non-coding RNA (lncRNA) PTENP1 is a pseudogene of phosphatase and tensin homologue deleted on chromosome ten (PTEN), has been implicated in smooth muscle cell (SMC) proliferation and apoptosis. PTENP1 is the pseudogene of PTEN. However, it is unclear whether and how PTENP1 functions in the proliferation and apoptosis of human aortic SMCs (HASMCs). Here, we hypothesised that PTENP1 inhibits HASMC proliferation and enhances apoptosis by promoting PTEN expression. PCR analysis and Western blot assays respectively showed that both PTENP1 and PTEN were up-regulated in human aortic dissection (AD) samples. PTENP1 overexpression significantly increased the protein expression of PTEN, promoted apoptosis and inhibited the proliferation of HASMCs. PTENP1 silencing exhibited the opposite effects and mitigated H2O2-induced apoptosis of HASMCs. In an angiotensin II (Ang II)-induced mouse aortic aneurysm (AA) model, PTENP1 overexpression potentiated aortic SMC apoptosis, exacerbated aneurysm formation. Mechanistically, RNA pull-down assay and a series of luciferase reporter assays using miR-21 mimics or inhibitors identified PTENP1 as a molecular sponge for miR-21 to endogenously compete for the binding between miR-21 and the PTEN transcript, releasing PTEN expression. This finding was further supported by in vitro immunofluorescent evidence showing decreased cell apoptosis upon miR-21 mimic administration under baseline PTENP1 overexpression. Ex vivo rescue of PTEN significantly mitigated the SMC apoptosis induced by PTENP1 overexpression. Finally, Western blot assays showed substantially reduced Akt phosphorylation and cyclin D1 and cyclin E levels with up-regulated PTENP1 in HASMCs. Our study identified PTENP1 as a mediator of HASMC homeostasis and suggests that PTENP1 is a potential target in AD or AA intervention.
Evidence from stellar rotation for early disc dispersal owing to close companions
Authors: Messina S. 
Published: June 2019

Context. Young (≲600 Myr) low-mass stars (M ≲ 1 M⊙) of equal mass exhibit a distribution of rotation periods. At the very early phases of stellar evolution, this distribution is set by the star-disc locking mechanism, which forces stars to rotate at the same rate as the inner edge of the disc. The primordial disc lifetime and consequently the duration of the disc-locking mechanism, can be significantly shortened by the presence of a close companion, making the rotation period distribution of close binaries different from that of either single stars or wide binaries.
Aims. We use new data to investigate and better constrain the range of ages, the components separation, and the mass ratio dependence at which the rotation period distribution has been significantly affected by the disc dispersal that is enhanced by close companions.
Methods. We select a sample of close binaries in the Upper Scorpius association (age ∼8 Myr) whose components have measured the separation and the rotation periods and compare their period distribution with that of coeval stars that are single stars.
Results. We find that components of close binaries have, on average, rotation periods that are shorter than those of single stars. More precisely, binaries with approximately equal-mass components (0.9 ≤ M2/M1 ≤ 1.0) have rotation periods that are shorter than those of single stars by ∼0.4 d on average; the primary and secondary components of binaries with smaller mass ratios (0.8 < M2/M1 < 0.9) have rotation periods that are shorter than those of single stars by ∼1.9 d and ∼1.0 d on average, respectively. A comparison with the older 25 Myr β Pictoris association shows that whereas in the latter, all close binaries with projected separation ρ ≤ 80 AU rotate faster than single stars, in the Upper Scorpius this is only the case for about 70% of stars.
Conclusions. We interpret the enhanced rotation in close binaries with respect to single stars as the consequence of an early disc dispersal induced by the presence of close companions. The enhanced rotation suggests that disc dispersal timescales are longest for single stars and shorter for close binaries.
Non-LTE analysis of K I in late-type stars
Authors: Reggiani Henrique , Amarsi Anish M. , Lind Karin , Barklem Paul S. , Zatsarinny Oleg , Bartschat Klaus , Fursa Dmitry V. , Bray Igor , Spina Lorenzo , Meléndez Jorge 
Published: June 2019

Context. Older models of Galactic chemical evolution (GCE) predict [K/Fe] ratios as much as 1 dex lower than those inferred from stellar observations. Abundances of potassium are mainly based on analyses of the 7698 Å resonance line, and the discrepancy between GCE models and observations is in part caused by the assumption of local thermodynamic equilibrium (LTE) in spectroscopic analyses.
Aims. We study the statistical equilibrium of K I, focusing on the non-LTE effects on the 7698 Å line. We aim to determine how non-LTE abundances of potassium can improve the analysis of its chemical evolution, and help to constrain the yields of GCE models.
Methods. We construct a new model K I atom that employs the most up-to-date atomic data. In particular, we calculate and present inelastic e+K collisional excitation cross-sections from the convergent close-coupling (CCC) and the B-Spline R-matrix (BSR) methods, and H+K collisions from the two-electron model (LCAO). We constructed a fine, extended grid of non-LTE abundance corrections based on 1D MARCS models that span 4000 < Teff∕K < 8000, 0.50 < log g < 5.00, − 5.00 < [Fe/H] < + 0.50, and applied the corrections to potassium abundances extracted from the literature.
Results. In concordance with previous studies, we find severe non-LTE effects in the 7698 Å line. The line is stronger in non-LTE and the abundance corrections can reach approximately − 0.7 dex for solar-metallicity stars such as Procyon. We determine potassium abundances in six benchmark stars, and obtain consistent results from different optical lines. We explore the effects of atmospheric inhomogeneity by computing for the first time a full 3D non-LTE stellar spectrum of K I lines for a test star. We find that 3D modeling is necessary to predict a correct shape of the resonance 7698 Å line, but the line strength is similar to that found in 1D non-LTE.
Conclusions. Our non-LTE abundance corrections reduce the scatter and change the cosmic trends of literature potassium abundances. In the regime [Fe/H] ≲−1.0 the non-LTE abundances show a good agreement with the GCE model with yields from rotating massive stars. The reduced scatter of the non-LTE corrected abundances of a sample of solar twins shows that line-by-line differential analysis techniques cannot fully compensate for systematic LTE modelling errors; the scatter introduced by such errors introduces a spurious dispersion to K evolution.
Temporal evolution and correlations of optical activity indicators measured in Sun-as-a-star observations
Authors: Maldonado J. , Phillips D. F. , Dumusque X. , Collier Cameron A. , Haywood R. D. , Lanza A. F. , Micela G. , Mortier A. , Saar S. H. , Sozzetti A. , Rice K. , Milbourne T. , Cecconi M. , Cegla H. M. , Cosentino R. , Costes J. , Ghedina A. , Gonzalez M. , Guerra J. , Hernández N. , Li C.-H. , Lodi M. , Malavolta L. , Molinari E. , Pepe F. , Piotto G. , Poretti E. , Sasselov D. , San Juan J. , Thompson S. , Udry S. , Watson C. 
Published: June 2019

Context. Understanding stellar activity in solar-type stars is crucial for the physics of stellar atmospheres as well as for ongoing exoplanet programmes.
Aims. We aim to test how well we understand stellar activity using our own star, the Sun, as a test case.
Methods. We performed a detailed study of the main optical activity indicators (Ca II H & K, Balmer lines, Na I D1 D2, and He I D3) measured for the Sun using the data provided by the HARPS-N solar-telescope feed at the Telescopio Nazionale Galileo. We made use of periodogram analyses to study solar rotation, and we used the pool variance technique to study the temporal evolution of active regions. The correlations between the different activity indicators as well as the correlations between activity indexes and the derived parameters from the cross-correlation technique are analysed. We also study the temporal evolution of these correlations and their possible relationship with indicators of inhomogeneities in the solar photosphere like sunspot number or radio flux values.
Results. The value of the solar rotation period is found in all the activity indicators, with the only exception being Hδ. The derived values vary from 26.29 days (Hγ line) to 31.23 days (He I). From an analysis of sliding periodograms we find that in most of the activity indicators the spectral power is split into several “bands” of periods around 26 and 30 days. They might be explained by the migration of active regions between the equator and a latitude of ∼30°, spot evolution, or a combination of both effects. A typical lifetime of active regions of approximately ten rotation periods is inferred from the pooled variance diagrams, which is in agreement with previous works. We find that Hα, Hβ, Hγ, Hϵ, and He I show a significant correlation with the S index. Significant correlations between the contrast, bisector span, and the heliocentric radial velocity with the activity indexes are also found. We show that the full width at half maximum, the bisector, and the disc-integrated magnetic field correlate with the radial velocity variations. The correlation of the S index and Hα changes with time, increasing with larger sun spot numbers and solar irradiance. A similar tendency with the S index and radial velocity correlation is also present in the data.
Conclusions. Our results are consistent with a scenario in which higher activity favours the correlation between the S index and the Hα activity indicators and between the S index and radial velocity variations.
Structural and Functional Basis of Plasminogen Activation by Staphylokinase
Authors: Jespers L. , Vanwetswinkel S. , Lijnen H. R. , Van Herzeele N. , Van Hoef B. , Demarsin E. , Collen D. , De Maeyer M. 
Published: June 2019

SummaryStaphylokinase (Sak), a 15.5-kDa bacterial protein, forms a complex with human plasmin, which in turn activates other plasminogen molecules to plasmin. Three recombinant DNA-based approaches, (i) site directed substitution with alanine, (ii) search for proximity relationships at the complex interface, and (iii) active-site accessibility to protease inhibitors have been used to deduce a coherent docking model of the crystal structure of Sak on the homology-based model of micro-plasmin (μPli), the serine protease domain of plasmin. Sak binding on μPli is primarily mediated by two surface-exposed loops, loops 174 and 215, at the rim of the active-site cleft, while the binding epitope of Sak on μPli involves several residues located in the flexible NH2-terminal arm and in the five-stranded mixed β-sheet. Several Sak residues located within the unique μ-helix and the β2 strand do not contribute to the binding epitope but are essential to induce plasminogen activating potential in the Sak:μPli complex. These residues form a topologically distinct activation epitope, which, upon binding of Sak to the catalytic domain of μPli, protrudes into a broad groove near the catalytic triad of μPli, thereby generating a competent binding pocket for micro-plasminogen (μPlg), which buries approximately 2500 Å of the Sak:μPli complex upon binding. This structural and functional model may serve as a template for the design of improved Sak-derived thrombolytic agents. Following the completion and presentation of the present study, the deduced Sak:μPli:μPlg complex was fully confirmed by X-ray crystallography, which further illustrates the power and potential of the present approach.
Differential Inhibition of Thrombin Activity and Thrombin Generation by a Synthetic Direct Thrombin Inhibitor (Napsagatran, Ro 46-6240) and Unfractionated Heparin in Patients with Deep Vein Thrombosis
Authors: Bounameaux Henri , Ehringer Herbert , Gast Alain , Hulting Johan , Rasche Herbert , Rapold Hans J. , Reber Guido , Tschopp Thomas B. ,   ADVENT Investigators
Published: June 2019

Summary
Background: Direct thrombin inhibitors belong to a new class of antithrombotic drugs whose effects on blood coagulation in vivo in patients suffering from acute thrombotic conditions have not yet been fully explored. Methods and Results: One hundred and five patients with acute proximal deep-vein thrombosis were randomized to receive a continuous intravenous infusion of napsagatran, a novel synthetic thrombin inhibitor, at a fixed dose of 5 mg/h (n = 36) or 9 mg/h (n = 25) for five days, or APTT-adjusted unfractionated heparin (UFH, n = 44) for the same time. In these patients, thrombin activity and thrombin generation could be assessed by measuring thrombin-antithrombin III complexes (TAT) and prothrombin fragment 1+2 (F1+2), respectively, on three occasions. At baseline, TAT and F1+2 did not differ among the three groups. On Day 2 (steady state), TAT significantly decreased in all groups, and the decrease was significantly more pronounced in the patients given higher-dose napsagatran. F1+2 decreased significantly only in UFH-treated patients. Two hours after cessation of the infusion, the TAT levels increased in the two napsagatran groups but not in the UFH group, whilst F1+2 went back to the baseline levels in the napsagatran-treated patients but remained low in the UFH-treated patients. There was no rebound effect. Conclusions: The data presented suggest that direct thrombin inhibition with napsagatran at 9 mg/h is more potent than UFH in attenuating thrombin activity, but is less potent than UFH in inhibiting thrombin generation. The real significance of these findings will have to be substantiated in further trials with clinically relevant endpoints.