bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Nonlinear Statistical Model for Extracting a Climatic Signal From Glacier Mass Balance Measurements
Authors: Vincent C. , Soruco A. , Azam M. F. , Basantes‐Serrano R. , Jackson M. , Kjøllmoen B. , Thibert E. , Wagnon P. , Six D. , Rabatel A. , Ramanathan A. , Berthier E. , Cusicanqui D. , Vincent P. , Mandal A. 
Published: September 2018

AbstractUnderstanding changes in glacier mass balances is essential for investigating climate changes. However, glacier‐wide mass balances determined from geodetic observations do not provide a relevant climatic signal as they depend on the dynamic response of the glaciers. In situ point mass balance measurements provide a direct signal but show a strong spatial variability that is difficult to assess from heterogeneous in situ measurements over several decades. To address this issue, we propose a nonlinear statistical model that takes into account the spatial and temporal changes in point mass balances. To test this model, we selected four glaciers in different climatic regimes (France, Bolivia, India, and Norway) for which detailed point annual mass balance measurements were available over a large elevation range. The model extracted a robust and consistent signal for each glacier. We obtained explained variances of 87.5, 90.2, 91.3, and 75.5% on Argentière, Zongo, Chhota Shigri, and Nigardsbreen glaciers, respectively. The standard deviations of the model residuals are close to measurement uncertainties. The model can also be used to detect measurement errors. Combined with geodetic data, this method can provide a consistent glacier‐wide annual mass balance series from a heterogeneous network. This model, available to the whole community, can be used to assess the impact of climate change in different regions of the world from long‐term mass balance series.
Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy
Authors: Bernhardt M. , Nicolas J.-D. , Osterhoff M. , Mittelstädt H. , Reuss M. , Harke B. , Wittmeier A. , Sprung M. , Köster S. , Salditt T. 
Published: September 2018

AbstractWe present a correlative microscopy approach for biology based on holographic X-ray imaging, X-ray scanning diffraction, and stimulated emission depletion (STED) microscopy. All modalities are combined into the same synchrotron endstation. In this way, labeled and unlabeled structures in cells are visualized in a complementary manner. We map out the fluorescently labeled actin cytoskeleton in heart tissue cells and superimpose the data with phase maps from X-ray holography. Furthermore, an array of local far-field diffraction patterns is recorded in the regime of small-angle X-ray scattering (scanning SAXS), which can be interpreted in terms of biomolecular shape and spatial correlations of all contributing scattering constituents. We find that principal directions of anisotropic diffraction patterns coincide to a certain degree with the actin fiber directions and that actin stands out in the phase maps from holographic recordings. In situ STED recordings are proposed to formulate models for diffraction data based on co-localization constraints.
Olfactory receptor OR2AT4 regulates human hair growth
Authors: Chéret Jérémy , Bertolini Marta , Ponce Leslie , Lehmann Janin , Tsai Teresa , Alam Majid , Hatt Hanns , Paus Ralf 
Published: September 2018

AbstractOlfactory receptors are expressed by different cell types throughout the body and regulate physiological cell functions beyond olfaction. In particular, the olfactory receptor OR2AT4 has been shown to stimulate keratinocyte proliferation in the skin. Here, we show that the epithelium of human hair follicles, particularly the outer root sheath, expresses OR2AT4, and that specific stimulation of OR2AT4 by a synthetic sandalwood odorant (Sandalore®) prolongs human hair growth ex vivo by decreasing apoptosis and increasing production of the anagen-prolonging growth factor IGF-1. In contrast, co-administration of the specific OR2AT4 antagonist Phenirat® and silencing of OR2AT4 inhibit hair growth. Together, our study identifies that human hair follicles can engage in olfactory receptor-dependent chemosensation and require OR2AT4-mediated signaling to sustain their growth, suggesting that olfactory receptors may serve as a target in hair loss therapy.
Modular engineering to increase intracellular NAD(H/+) promotes rate of extracellular electron transfer of Shewanella oneidensis
Authors: Li Feng , Li Yuan-Xiu , Cao Ying-Xiu , Wang Lei , Liu Chen-Guang , Shi Liang , Song Hao 
Published: September 2018

AbstractThe slow rate of extracellular electron transfer (EET) of electroactive microorganisms remains a primary bottleneck that restricts the practical applications of bioelectrochemical systems. Intracellular NAD(H/+) (i.e., the total level of NADH and NAD+) is a crucial source of the intracellular electron pool from which intracellular electrons are transferred to extracellular electron acceptors via EET pathways. However, how the total level of intracellular NAD(H/+) impacts the EET rate in Shewanella oneidensis has not been established. Here, we use a modular synthetic biology strategy to redirect metabolic flux towards NAD+ biosynthesis via three modules: de novo, salvage, and universal biosynthesis modules in S. oneidensis MR-1. The results demonstrate that an increase in intracellular NAD(H/+) results in the transfer of more electrons from the increased oxidation of the electron donor to the EET pathways of S. oneidensis, thereby enhancing intracellular electron flux and the EET rate.
Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration
Authors: Paul Sharan , Dansithong Warunee , Figueroa Karla P. , Scoles Daniel R. , Pulst Stefan M. 
Published: September 2018

AbstractSpinocerebellar ataxia type 2 (SCA2) is a neurodegenerative disease caused by expansion of polyglutamine tract in the ATXN2 protein. We identified Staufen1 (STAU1) as an interactor of ATXN2, and showed elevation in cells from SCA2 patients, amyotrophic lateral sclerosis (ALS) patients, and in SCA2 mouse models. We demonstrated recruitment of STAU1 to mutant ATXN2 aggregates in brain tissue from patients with SCA2 human brain and in an SCA2 mouse model, and association of STAU1 elevation with dysregulation of SCA2-related transcript abundances. Targeting STAU1 in vitro by RNAi restored PCP2 transcript levels and lowering mutant ATXN2 also normalized STAU1 levels. Reduction of Stau1 in vivo improved motor behavior in an SCA2 mouse model, normalized the levels of several SCA2-related proteins, and reduced aggregation of polyglutamine-expanded ATXN2. These findings suggest a function for STAU1 in aberrant RNA metabolism associated with ATXN2 mutation, suggesting STAU1 is a possible novel therapeutic target for SCA2.
T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells
Authors: Kim Hye-Ran , Mun YeVin , Lee Kyung-Sik , Park Yoo-Jin , Park Jeong-Su , Park Jin-Hwa , Jeon Bu-Nam , Kim Chang-Hyun , Jun Youngsoo , Hyun Young-Min , Kim Minsoo , Lee Sang-Myeong , Park Chul-Seung , Im Sin-Hyeog , Jun Chang-Duk 
Published: September 2018

AbstractMicrovilli on T cells have been proposed to survey surfaces of antigen-presenting cells (APC) or facilitate adhesion under flow; however, whether they serve essential functions during T cell activation remains unclear. Here we show that antigen-specific T cells deposit membrane particles derived from microvilli onto the surface of cognate antigen-bearing APCs. Microvilli carry T cell receptors (TCR) at all stages of T cell activation and are released as large TCR-enriched, T cell microvilli particles (TMP) in a process of trogocytosis. These microvilli exclusively contain protein arrestin-domain-containing protein 1, which is directly involved in membrane budding and, in combination with vacuolar protein-sorting-associated protein 4, transforms large TMPs into smaller, exosome-sized TMPs. Notably, TMPs from CD4+T cells are enriched with LFA-2/CD2 and various cytokines involved in activating dendritic cells. Collectively, these results demonstrate that T cell microvilli constitute “immunological synaptosomes” that carry T cell messages to APCs.
Trojan Horse nanotheranostics with dual transformability and multifunctionality for highly effective cancer treatment
Authors: Xue Xiangdong , Huang Yee , Bo Ruonan , Jia Bei , Wu Hao , Yuan Ye , Wang Zhongling , Ma Zhao , Jing Di , Xu Xiaobao , Yu Weimin , Lin Tzu-yin , Li Yuanpei 
Published: September 2018

AbstractNanotheranostics with integrated diagnostic and therapeutic functions show exciting potentials towards precision nanomedicine. However, targeted delivery of nanotheranostics is hindered by several biological barriers. Here, we report the development of a dual size/charge- transformable, Trojan-Horse nanoparticle (pPhD NP) for delivery of ultra-small, full active pharmaceutical ingredients (API) nanotheranostics with integrated dual-modal imaging and trimodal therapeutic functions. pPhD NPs exhibit ideal size and charge for drug transportation. In tumour microenvironment, pPhD NPs responsively transform to full API nanotheranostics with ultra-small size and higher surface charge, which dramatically facilitate the tumour penetration and cell internalisation. pPhD NPs enable visualisation of biodistribution by near-infrared fluorescence imaging, tumour accumulation and therapeutic effect by magnetic resonance imaging. Moreover, the synergistic photothermal-, photodynamic- and chemo-therapies achieve a 100% complete cure rate on both subcutaneous and orthotopic oral cancer models. This nanoplatform with powerful delivery efficiency and versatile theranostic functions shows enormous potentials to improve cancer treatment.
Decisions are expedited through multiple neural adjustments spanning the sensorimotor hierarchy
Authors: Steinemann Natalie A. , O’Connell Redmond G. , Kelly Simon P. 
Published: September 2018

AbstractWhen decisions are made under speed pressure, “urgency” signals elevate neural activity toward action-triggering thresholds independent of the sensory evidence, thus incurring a cost to choice accuracy. While urgency signals have been observed in brain circuits involved in preparing actions, their influence at other levels of the sensorimotor pathway remains unknown. We used a novel contrast-comparison paradigm to simultaneously trace the dynamics of sensory evidence encoding, evidence accumulation, motor preparation, and muscle activation in humans. Results indicate speed pressure impacts multiple sensorimotor levels but in crucially distinct ways. Evidence-independent urgency was applied to cortical action-preparation signals and downstream muscle activation, but not directly to upstream levels. Instead, differential sensory evidence encoding was enhanced in a way that partially countered the negative impact of motor-level urgency on accuracy, and these opposing sensory-boost and motor-urgency effects had knock-on effects on the buildup and pre-response amplitude of a motor-independent representation of cumulative evidence.
A rationally designed JAZ subtype-selective agonist of jasmonate perception
Authors: Takaoka Yousuke , Iwahashi Mana , Chini Andrea , Saito Hiroaki , Ishimaru Yasuhiro , Egoshi Syusuke , Kato Nobuki , Tanaka Maho , Bashir Khurram , Seki Motoaki , Solano Roberto , Ueda Minoru 
Published: September 2018

AbstractThe phytohormone 7-iso-(+)-jasmonoyl-L-isoleucine (JA-Ile) mediates plant defense responses against herbivore and pathogen attack, and thus increases plant resistance against foreign invaders. However, JA-Ile also causes growth inhibition; and therefore JA-Ile is not a practical chemical regulator of plant defense responses. Here, we describe the rational design and synthesis of a small molecule agonist that can upregulate defense-related gene expression and promote pathogen resistance at concentrations that do not cause growth inhibition inArabidopsis. By stabilizing interactions between COI1 and JAZ9 and JAZ10 but no other JAZ isoforms, the agonist leads to formation of JA-Ile co-receptors that selectively activate the JAZ9-EIN3/EIL1-ORA59 signaling pathway. The design of a JA-Ile agonist with high selectivity for specific protein subtypes may help promote the development of chemical regulators that do not cause a tradeoff between growth and defense.
Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
Authors: Hamon Rhys , Tran Hai B. , Roscioli Eugene , Ween Miranda , Jersmann Hubertus , Hodge Sandra 
Published: September 2018

AbstractBushfires are increasing in frequency and severity worldwide. Bushfire smoke contains organic/inorganic compounds including aldehydes and acrolein. We described suppressive effects of tobacco smoke on the phagocytic capacity of airway macrophages, linked to secondary necrosis of uncleared apoptotic epithelial cells, persistence of non-typeable H. influenzae (NTHi), and inflammation. We hypothesised that bushfire smoke extract (BFSE) would similarly impair macrophage function. THP-1 or monocyte-derived macrophages (MDM) were exposed to 1–10% BFSE prepared from foliage of 5 common Australian native plants (genus Acacia or Eucalyptus), or 10% cigarette smoke extract (CSE). Phagocytic recognition receptors were measured by flow cytometry; pro-inflammatory cytokines and caspase 1 by immunofluorescence or cytometric bead array; viability by LDH assay; and capsase-3/PARP by western blot. BFSE significantly decreased phagocytosis of apoptotic cells or NTHi by both THP-1 macrophages and MDM vs air control, consistent with the effects of CSE. BFSE significantly decreased MDM expression of CD36, CD44, SR-A1, CD206 and TLR-2 and increased active IL-1β, caspase-1 and secreted IL-8. BFSE dose-dependently decreased THP-1 macrophage viability (5-fold increase in LDH at 10%) and significantly increased active caspase-3. BFSE impairs macrophage function to a similar extent as CSE, highlighting the need for further research, especially in patients with pre-existing lung disease.
X-ray Fluorescence Nanotomography of Single Bacteria with a Sub-15 nm Beam
Authors: Victor Tiffany W. , Easthon Lindsey M. , Ge Mingyuan , O’Toole Katherine H. , Smith Randy J. , Huang Xiaojing , Yan Hanfei , Allen Karen N. , Chu Yong S. , Miller Lisa M. 
Published: September 2018

AbstractX-ray Fluorescence (XRF) microscopy is a growing approach for imaging the trace element concentration, distribution, and speciation in biological cells at the nanoscale. Moreover, three-dimensional nanotomography provides the added advantage of imaging subcellular structure and chemical identity in three dimensions without the need for staining or sectioning of cells. To date, technical challenges in X-ray optics, sample preparation, and detection sensitivity have limited the use of XRF nanotomography in this area. Here, XRF nanotomography was used to image the elemental distribution in individual E. coli bacterial cells using a sub-15 nm beam at the Hard X-ray Nanoprobe beamline (HXN, 3-ID) at NSLS-II. These measurements were simultaneously combined with ptychography to image structural components of the cells. The cells were embedded in small (3–20 µm) sodium chloride crystals, which provided a non-aqueous matrix to retain the three-dimensional structure of the E. coli while collecting data at room temperature. Results showed a generally uniform distribution of calcium in the cells, but an inhomogeneous zinc distribution, most notably with concentrated regions of zinc at the polar ends of the cells. This work demonstrates that simultaneous two-dimensional ptychography and XRF nanotomography can be performed with a sub-15 nm beam size on unfrozen biological cells to co-localize elemental distribution and nanostructure simultaneously.
Characterizing thermal-oxidation behaviors of nuclear graphite by combining O2 supply and micro surface area of graphite
Authors: Zhou Yangping , Dong Yujie , Yin Huaqiang , Li Zhengcao , Yan Rui , Li Dianbin , Gu Zhengwei , Sun Ximing , Shi Lei , Zhang Zuoyi 
Published: September 2018

AbstractThe effects of different parameters on oxidation rate are non-linear, interactive and diversified in which the change of adequacy of O2 supply is an important indicator. The influence of microstructure on oxidation rate became stronger worsening the fitting linearity to calculate the activation energy based on present method with the decreased adequacy of O2 supply due to the increase of temperature, the decrease of gas flow rate, etc. Here, we proposed a method to characterize thermal-oxidation behaviors of nuclear graphite by combining O2 supply and micro surface area of graphite. The proposed method improved the linearity and reduced the standard error of Arrhenius plots of oxidized graphite IG-110 (10 L/min reactant gas) and ET-10 (0.2 L/min reactant gas). The value of activation energy of graphite IG-110 oxidized under ASTM D7542 condition is calculated as 220 kJ/mol by this method echoing the results of previous studies with sufficient O2 supply. For the conditions with less O2 supply at low gas flow rate and/or high temperature, the change of microstructure of oxidized graphite should be obtained as an important factor influencing oxidation rate of graphite.
Dual action of L-Lactate on the activity of NR2B-containing NMDA receptors: from potentiation to neuroprotection
Authors: Jourdain P. , Rothenfusser K. , Ben-Adiba C. , Allaman I. , Marquet P. , Magistretti P. J. 
Published: September 2018

AbstractL-Lactate is a positive modulator of NMDAR-mediated signaling resulting in plasticity gene induction and memory consolidation. However, L-Lactate is also able to protect neurons against excito-toxic NMDAR activity, an indication of a mitigating action of L-Lactate on NMDA signaling. In this study, we provide experimental evidence that resolves this apparent paradox. Transient co-application of glutamate/glycine (1 μM/100 μM; 2 min) in primary cultures of mouse cortical neurons triggers a NMDA-dependent Ca2+ signal positively modulated by L-Lactate (10 mM) or DTT (1 mM) but decreased by Pyruvate (10 mM). This L-Lactate and DTT-induced potentiation is blocked by Ifenprodil (2 μM), a specific blocker of NMDARs containing NR2B sub-units. In contrast, co-application of glutamate/glycine (1 mM/100 μM; 2 min) elicits a NMDAR-dependent excitotoxic death in 49% of neurons. L-Lactate and Pyruvate significantly reduce this rate of cell death processes (respectively to 23% and 9%) while DTT has no effect (54% of neuronal death). This L-Lactate-induced neuroprotection is blocked by carbenoxolone and glibenclamide, respectively blockers of pannexins and KATP. In conclusion, our results show that L-Lactate is involved in two distinct and independent pathways defined as NMDAR-mediated potentiation pathway (or NADH pathway) and a neuroprotective pathway (or Pyruvate/ATP pathway), the prevalence of each one depending on the strength of the glutamatergic stimulus.
Facile and scalable production of heterostructured ZnS-ZnO/Graphene nano-photocatalysts for environmental remediation
Authors: Lonkar Sunil P. , Pillai Vishnu V. , Alhassan Saeed M. 
Published: September 2018

AbstractA facile and eco-friendly strategy is described for the synthesis of ZnS-ZnO/graphene heterostructured nano-photocatalysts for the first time. This solvent-free and technologically scalable method involves solid-state mixing of graphite oxide (GO), Zn salt and surfeit yet non-toxic elemental sulfur using ball-milling followed by thermal annealing. The as-formed hybrids are composed of uniformly distributed in-situ formed ZnS-ZnO nanoparticles simultaneously within the thermally reduced GO (graphene) matrix. A series of hybrid compositions with varying content of ZnS/ZnO and graphene were prepared and thoroughly characterized. Further, the effect of heterostructure composition on the photocatalytic properties was investigated under visible-light illumination. The synergistic ZnS-ZnO/graphene hybridization promoted the band-gap narrowing compared to the pristine ZnS nanoparticles. The ZnS:ZnO composition was controlled by graphite oxide under thermal treatment and observed to be a crucial factor in enhancement of photocatalytic activity. As a proof of concept, the phase optimized and surface enhanced ZnS-ZnO/graphene nano-photocatalysts was tested towards visible light driven photocatalytic degradation of environmentally harmful organic dyes and toxic phenol molecules from aqueous media. The presented cost-effective strategy provides high potential in large-scale production of heterostructured nano-photocatalysts for environmental remediation and photocatalytic greener production of hydrogen.
Canine morbillivirus (canine distemper virus) with concomitant canine adenovirus, canine parvovirus-2, and Neospora caninum in puppies: a retrospective immunohistochemical study
Authors: Headley Selwyn A. , Oliveira Thalita E. S. , Pereira Alfredo H. T. , Moreira Jéssica R. , Michelazzo Mariana M. Z. , Pires Bárbara G. , Marutani Victor Hugo B. , Xavier Ana A. C. , Di Santis Giovana W. , Garcia João L. , Alfieri Amauri A. 
Published: September 2018

AbstractA retrospective immunohistochemical study was designed to investigate the frequency of concomitant traditional infectious disease pathogens in puppies that died suddenly and review the aspects of associated pathogenesis. Fifteen puppies were evaluated; the pathology reports and histopathologic slides of these animals were reviewed to determine the pattern of histopathologic lesions. The intralesional identification of antigens of canine (distemper) morbillivirus (CDV), canine adenovirus-1 and -2 (CAdV-1 and -2), canine parvovirus-2 (CPV-2), Toxoplasma gondii, and Neospora caninum was evaluated by IHC within the histopathologic patterns observed. All puppies contained CDV nucleic acid by molecular testing. The most frequent histopathologic patterns were intestinal crypt necrosis (n = 8), white matter cerebellar demyelination (n = 7), necrohaemorrhagic hepatitis (n = 7), interstitial pneumonia (n = 7), and gallbladder oedema (n = 5). All puppies contained intralesional antigens of CDV in multiple tissues resulting in singular (n = 3), and concomitant dual (n = 3), triple (n = 5) and quadruple (n = 4) infections by CAdV-1, and -2, CPV-2, and N. caninum; T. gondii was not identified. Concomitant infections by CDV was observed with N. caninum (100%; 1/1), CPV-2 (100%; 8/8), CAdV-1 (100%; 8/8), and CAdV-2 (100%; 8/8). Intralesional antigens of CDV and not CAdV-1 were identified in cases of gallbladder oedema. The “blue eye” phenomenon was histologically characterized by corneal oedema and degenerative lesions to the corneal epithelium, without inflammatory reactions.