bluuebunny/crossref_metadata_2025_split huggingface dataset:
Septin 2/6/7 complexes tune microtubule plus end growth and EB1 binding in a concentration- and filament-dependent manner
Authors: Nakos Konstantinos , Radler Megan R. , Spiliotis Elias T. 
Published: May 2019

AbstractSeptins are filamentous GTP-binding proteins, which affect microtubule (MT) dependent functions including membrane trafficking and cell division, but their precise role in MT dynamics is poorly understood. Here, in vitro reconstitution of MT dynamics with SEPT2/6/7, the minimal subunits of septin heteromers, shows that SEPT2/6/7 has a biphasic concentration-dependent effect on MT growth. Lower concentrations of SEPT2/6/7 enhance MT plus end growth and elongation, while higher and intermediate concentrations inhibit and pause plus end growth, respectively. We show that SEPT2/6/7 has a 1.5-fold preference for GTP-over GDP-bound MT lattice, and competes with EB1 for binding to GTPγS-stabilized MTs, which mimic the EB1-preferred GDP-Pi state of polymerized tubulin. Strikingly, SEPT2/6/7 triggers EB1 dissociation from plus end tips in cis by binding to the MT lattice and in trans when MT plus ends collide with SEPT2/6/7 filaments. At these intersections, SEPT2/6/7 filaments were more potent barriers than actin filaments in pausing MT growth and dissociating EB1 in vitro and in live cells. These data demonstrate that SEPT2/6/7 complexes and filaments can directly impact MT plus end growth and the tracking of plus end-binding proteins, and thereby may facilitate the capture of MT plus ends at intracellular sites of septin enrichment.Highlight Summary for eTOCKnowledge of septin roles in MT dynamics is poor and confounded by knockdown studies. Here, in vitro reconstitution assays show concentration-dependent effects of SEPT2/6/7 on MT plus end growth, pausing and EB1 tracking. We found that SEPT2/6/7 filaments are potent than actin in pausing MT growth and dissociating EB1 from intersecting plus ends.
Cryo-EM structure of the MinCD copolymeric filament from Pseudomonas aeruginosa at 3.1 Å resolution
Authors: Szewczak-Harris Andrzej , Wagstaff James , Löwe Jan 
Published: May 2019

AbstractPositioning of the division site in many bacterial species relies on the MinCDE system, which prevents the cytokinetic Z-ring from assembling anywhere but the mid-cell, through an oscillatory diffusion-reaction mechanism. MinD dimers bind to membranes and, via their partner MinC, inhibit the polymerisation of cell division protein FtsZ into the Z-ring. MinC and MinD form polymeric assemblies in solution and on cell membranes. Here, we report the high-resolution cryo-EM structure of the copolymeric filaments of Pseudomonas aeruginosa MinCD. The filaments consist of three protofilaments made of alternating MinC and MinD dimers. The MinCD protofilaments are almost completely straight and assemble as single protofilaments on lipid membranes, which we also visualised by cryo-EM.
Productivity and production risk effects of adopting drought-tolerant maize varieties in Zambia
Authors: Amondo Emily , Simtowe Franklin , Rahut Dil Bahadur , Erenstein Olaf 
Published: May 2019

PurposeProductivity and production risks affect the use of agricultural production practices and inputs, particularly in developing countries. This paper aims to investigate the effects of adopting drought-tolerant maize varieties (DTMVs) on farm productivity, yield variance and downside risk exposure of maize growing households of Zambia.Design/methodology/approachThe study uses household survey data collected from 11 maize producing districts of Eastern, Southern and Copperbelt provinces of Zambia using a structured questionnaire. The Antle’s flexible moment-based approach was used in specifying, estimating and testing a stochastic production function. The study further applied an endogenous switching regression model to control for both observable and unobservable sources of bias.FindingsThe study revealed that DTMV adoption increases maize yield by 15 per cent and reduces the risk of crop failure: reducing yield variance by 38 per cent and exposure to downside risk by 36 per cent.Originality/valueThis study establishes the benefits of DTMV adoption in Zambia with regards to productivity, yield stability and downside risk in the face of climate change. Results from this study underscore the need for more concerted efforts to scale-out DTMVs for both maize productivity enhancement and for risk mitigation against weather shocks.
Perceptions of climate change and water availability in the Mediterranean tourist sector
Authors: Torres-Bagur Maria , Ribas Palom Anna , Vila-Subirós Josep 
Published: May 2019

Purpose
The purpose of this paper is understand these perceptions and identify main problems associated to climate change in order to design effective mitigation and adaptation strategies to guarantee the sustainability of tourism and natural resources. The Mediterranean basin is a leading international tourist destination and one that is particularly vulnerable to the effects of climate change. Although these effects are largely known, in-depth studies of how they and the associated risks are perceived by key tourism stakeholders have not been performed.

Design/methodology/approach
Interviews were held with 31 hotels, campsites and rural lodgings in the Muga River basin in north-east Catalonia, in which both owners and managers were asked about their perceptions of climate change, effects and countermeasures.

Findings
Perceptions of climate change and its effects varied according to the type of establishment and location (coast, cities or inland). Significant differences were observed for perceptions of how responsibilities for implementing mitigation and adaptation measures to counter the effects of climate change, including water shortages, should be shared out between the main agents with an interest in guaranteeing the sustainability of tourism, namely, government bodies, tourist establishments and clients. The predominant opinion, however, was that the bulk of the responsibility should lie with government bodies.

Practical implications
Only when those responsible for running tourist establishments are aware of the risks of climate change, it will be possible to design and implement effective short-, medium- and long-term strategies aimed at strengthening the resilience of the tourist industry.

Originality/value
The originality of this study lies in its novel methodology and approach, which involved analyzing perceptions of climate change, including water shortages and its effects among owners and managers of different types of tourist accommodation establishments in distinct parts of the same area.
On the development of cat swarm metaheuristic using distributed learning strategies and the applications
Authors: Mohapatra Usha Manasi , Majhi Babita , Jagadev Alok Kumar 
Published: May 2019

Purpose
The purpose of this paper is to propose distributed learning-based three different metaheuristic algorithms for the identification of nonlinear systems. The proposed algorithms are experimented in this study to address problems for which input data are available at different geographic locations. In addition, the models are tested for nonlinear systems with different noise conditions. In a nutshell, the suggested model aims to handle voluminous data with low communication overhead compared to traditional centralized processing methodologies.

Design/methodology/approach
Population-based evolutionary algorithms such as genetic algorithm (GA), particle swarm optimization (PSO) and cat swarm optimization (CSO) are implemented in a distributed form to address the system identification problem having distributed input data. Out of different distributed approaches mentioned in the literature, the study has considered incremental and diffusion strategies.

Findings
Performances of the proposed distributed learning-based algorithms are compared for different noise conditions. The experimental results indicate that CSO performs better compared to GA and PSO at all noise strengths with respect to accuracy and error convergence rate, but incremental CSO is slightly superior to diffusion CSO.

Originality/value
This paper employs evolutionary algorithms using distributed learning strategies and applies these algorithms for the identification of unknown systems. Very few existing studies have been reported in which these distributed learning strategies are experimented for the parameter estimation task.
Towards more flexible approach to governance to allow innovation: the case of UK infrastructure
Authors: Sergeeva Natalya 
Published: May 2019

Purpose
The purpose of this paper is to explore the governance in project organising where owner organisations interface with the temporary project organisations that they initiate. This interface between the two types of organisation represents an opportunity for innovation.

Design/methodology/approach
In total, 25 narrative interviews were conducted with managers who work in permanent owner and operator organisations and temporary project organisations. It is combined with the analysis of textual narratives represented in institutional reports (APM, IPA, PMI).

Findings
The findings show that it is the flexible and balanced approach to governance that allows innovation to emerge. Strong capable innovative owners play crucial role in creating the corporate governance framework to allow innovation in projects.

Research limitations/implications
The current research presents narratives voiced by senior managers in permanent owner and operator organisations and temporary project organisations. The ways governance can be adjusted through the life cycle of major programmes require further a more longitudinal research investigation.

Practical implications
The practical benefits for the project management community is a better understanding of corporate governance in owner and operator organisations, the role of leadership and their narratives in governing processes, and the impact of strong governance on organisational performance and project deliverables.

Social implications
Senior managers socially constructed the meaning of governance through narratives. The author learn about practices of governance through the perspectives of those involved in decision making.

Originality/value
This paper contributes to project management theory in two ways: it provides insight into the practice of corporate governance; and it develops the application of narrative enquiry to project management research.
Consequences of customer experience quality on franchises and non-franchises models
Authors: Mainardes Emerson Wagner , Gomes Vinicius Costa Amorim , Marchiori Danilo , Correa Luis Eugenio , Guss Vinicius 
Published: May 2019

Purpose
The purpose of this paper is to verify the differences of the influence of customer experience quality on brand equity, brand trustworthiness, perceived quality, perceived risk and purchase intention of franchise customers and non-franchise customers.

Design/methodology/approach
After developing two questionnaires, the authors collected 523 responses from Brazilian franchise users (Questionnaire 1) and 574 of non-franchise users (Questionnaire 2). The authors proceed to a confirmatory factor analysis, based on covariance (CB-SEM). In order to compare the results between franchises and non-franchises, the authors have performed a multi-group analysis with support of AMOS.

Findings
The results show that customer experience quality of the franchise customers tends to result in a better purchase intention, giving indications of better quality and brand trustworthiness when compared to non-franchises. This comparison shows indications of the competitive advantage of franchises over non-franchises, justifying the investments that market companies have been making in the development of the customer experience quality.

Originality/value
The research contributes to the understanding of the impact of the customer experience quality on brand equity, brand trustworthiness, perceived quality, perceived risk and purchase intention that directly affects the performance of the franchises, empirically investigating the customer experience quality in the context of franchises using the adapted EXQ scale. Complementarily, it is compared with non-franchises to observe the differences between them.
The resolution of audit-detected misstatements
Authors: Ruhnke Klaus , Schmidt Martin 
Published: May 2019

PurposeThe value of an audit lies in its ability to detect and correct misstatements in financial statements. The purpose of this paper is to investigate the resolution of detected misstatements.Design/methodology/approachUsing data on a large sample of audit adjustments by a German Big 4 audit firm, this paper reports an analysis of the factors that influence the resolution of detected misstatements at the engagement level and at the level of the individual adjustment.FindingsThe auditor’s book-or-waive decision is influenced by client characteristics and characteristics of the audit adjustments that can be linked to client as well as auditor incentives to oppose (require) the recording of adjustments. Offsetting effects of the detected adjustments on client income play a key role in auditors’ decisions.Research limitations/implicationsThe results may not be generalizable to Non-Big 4 audit firms. The sample includes unlisted firms which are subject to mandatory audits in the European Union. Other potential factors may not have been captured in the study.Practical implicationsThe findings suggest that standard-setters and audit practice should give more attention to the offsetting effects of audit adjustments on income. The analyses at the two different levels could help practitioners to better structure their book-or-waive decisions.Originality/valueIn contrast to prior studies, the authors analyze the resolution of misstatements at the engagement level and the level of the individual adjustment. This design makes a methodological contribution by expanding the level of analysis of auditors’ book-or-waive decisions to include the engagement level. It further allows examining offsetting effects of different adjustments on client income, as well as the number and the total amount of detected adjustments.
Exploring linkages of performance with metacognition
Authors: Lyons Paul R. , Bandura Randall Paul 
Published: May 2019

PurposeIn this exploratory, correlational study the authors set out to demonstrate the relationships as well as inter-correlations among direct and indirect performance measures, along with measures of knowledge of cognition, and evaluation of cognition. The information helps inform manager learning and development. The purpose of this paper is twofold: first, primary purpose, to identify linkages of performance with individual’s efforts to improve their learning processes via metacognition; and second, secondary purpose, primarily for the benefit of practitioners, is the provision of detailed information regarding performance measures and practical measures of metacognition.Design/methodology/approachThe study made use of correlation analysis among performance measures and measures of metacognitive effort. The design is not intended to support cause and effect relationships, nor demonstrate the technical, predictive value of measures.FindingsA majority of associations among indirect performance measures with one another and with nearly all of the measures of knowledge of cognition, and evaluation of cognition were positive and significant (mostly at the 0.01 level). Findings offer broad support for the linkage of self-efficacy (SE), and core self-evaluation (CSE) with performance.Practical implicationsRelationships identified in this study may help practitioners alter and improve their practices/methods of identifying individuals who possess attributes that are highly related to performance and learning. The new knowledge may influence decisions about recruitment, selection and training.Originality/valueLittle research has focused on relationships among indirect performance indicators such as SE, CSE and established measures of metacognition. The present study helps to identify important relationships.
Social media for outbound leisure travel: a framework based on technology acceptance model (TAM)
Authors: Singh Snigdha , Srivastava Pallavi 
Published: May 2019

Purpose
The purpose of this paper is to explore the applicability of technology acceptance model (TAM) to explain the widespread acceptance and usage of social media (SM) for travel purposes by Indian outbound leisure travellers during their travel cycle.

Design/methodology/approach
The study has adopted a qualitative approach involving in-depth interviews of 30 respondents, who had taken at least one international vacation in the last 12 months. Content analysis was used to arrive at the conclusion.

Findings
Findings of the study are used to develop a conceptual model which upholds the validity of the TAM with perceived usefulness (PU) and perceived ease of use (PEU) as determinants of SM usage. The model has been extended to include perceived trust (PT) and social capital (SC) as important constructs to explain the travellers’ use of SM. Disposition readiness (DR) of the user towards SM was found to have an effect on all the four constructs.

Originality/value
Theoretically, this study suggests a modification to the existing TAM, which shows a unidirectional effect of PU and PEU on attitude. This study suggests that the effect would be bi-directional where the attitude (DR) of the traveller towards SM usage will affect PU and PEU. Furthermore, this study extends the TAM to include PT and SC as important constructs to explain SM usage by travellers. This study provides valuable insights into the hospitality and tourism sector which might help in further segmentation of travellers leading to more customised service offerings.
Non‐traumatic musculoskeletal pain in Western Australian hospital emergency departments: A clinical audit of the prevalence, management practices and evidence‐to‐practice gaps
Authors: Baker Briarley , Kessler Kenny , Kaiser Bronwyn , Waller Robert , Ingle Michael , Brambilla Simone , Viscardi Elena , Richards Karen , O'Sullivan Peter , Goucke Roger , Smith Anne , Yao Felix , Lin Ivan 
Published: May 2019

AbstractObjectivesMusculoskeletal pain (MSP) conditions are a leading cause of morbidity worldwide and a common reason for ED presentation. Little is currently known about non‐traumatic MSP (NTMSP) presenting to EDs. The present study described the prevalence and management practices of NTMSP in EDs.MethodsThe design was a retrospective clinical audit in two hospital EDs in Western Australia covering 3 months beginning 1 January 2016. We defined NTMSP as pain of musculoskeletal origin occurring in the absence of external force or excessive physical loading. The outcomes measured included: patient, condition and hospital‐episode characteristics, as well as management practices. Management practices were compared to recommended care derived from guideline recommendations. These included: assessment for red flags and psychosocial risk factors, appropriate use of diagnostic imaging, provision of patient education, administration and prescription of analgesic medication, and assessment of risk factors for opioid‐related harm.ResultsEight hundred and eighty‐eight patients were included in the present study. NTMSP accounted for 3.0% of all ED presentations. According to clinician documentation, red flag and psychosocial assessments were recorded in 73.3 and 10.5% of patients. Forty‐one percent of patients were referred for imaging, of which 39.7% were inconsistent with guideline recommendations. Education was recorded 52.0% of the time. At least one opioid medication was administered to 55.3% of patients and there was no documented assessment of risk factors for opioid‐related harm.ConclusionsNTMSP is a relatively common reason for ED presentation. Documented management practices are discordant with guideline recommendations. Strategies to improve the concordance between management and guideline recommendations are needed.
Voluntary Disclosures When There Is an Option to Delay Disclosure
Authors: Menon Rahul 
Published: May 2019

ABSTRACTWhat incentives drive managers to disclose immediately when they have an option to delay disclosures? I examine this question in a two‐period setting in which public news that is positively correlated with firm value arrives periodically. I show that, when the manager's likelihood of receiving information is independent of the public news, an informed manager is more likely to disclose immediately when the public news is good. This happens even as the disclosure threshold itself increases in the public news. My model provides a potential explanation for why managers have a higher propensity to provide earnings forecasts when current earnings are high. I also show that, even when disclosures are credible, the average price reaction to a voluntary disclosure is (i) decreasing in the magnitude of the public news and (ii) lower when the manager is more myopic. These results have potential implications for studies that use stock returns to measure the news contained in management disclosures.
Conserving orangutans one classroom at a time: evaluating the effectiveness of a wildlife education program for school‐aged children in Indonesia
Authors: Freund C. A. , Achmad M. , Kanisius P. , Naruri R. , Tang E. , Knott C. D. 
Published: May 2019

AbstractEnvironmental education is a cornerstone of many wildlife conservation programs, and measuring the impact of such education initiatives is imperative for evaluating their effectiveness in achieving conservation goals. For over 15 years, the Gunung Palung Orangutan Conservation Program (GPOCP) has been working to protect wild Bornean orangutans Pongo pygmaeus wurmbii living in and around Gunung Palung National Park in West Kalimantan, Indonesia. In 2015, we carried out a series of six Environmental Education Expeditions to communities in the remote districts surrounding the Park, engaging 1519 elementary (n = 770) and junior/senior high school (n = 749) students across 24 schools. Before and after engaging with our environmental education activities students were given surveys to assess their knowledge about and attitudes toward orangutans. We found that our educational activities led to 13.6–40.4% increases in student knowledge about orangutans and substantial shifts toward more positive attitudes about conservation, but that many students do not believe that they personally can make a difference. Empowerment may be a key need to link wildlife education to environmental action.