bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Phf16 and Pnpla3 as new adipogenic factors regulated by phytochemicals
Authors: Chang Seo‐Hyuk , Yun Ui Jeong , Choi Jin Hee , Kim Suji , Lee A Reum , Lee Dong Ho , Seo Min‐Ju , Panic Vanja , Villanueva Claudio J. , Song No‐Joon , Park Kye Won 
Published: September 2018

AbstractAdipocyte differentiation is controlled by multiple signaling pathways. To identify new adipogenic factors, C3H10T1/2 adipocytes were treated with previously known antiadipogenic phytochemicals (resveratrol, butein, sulfuretin, and fisetin) for 24 hours. Commonly regulated genes were then identified by transcriptional profiling analysis. Three genes (chemokine (C‐X‐C motif) ligand 1 [
Cxcl1], heme oxygenase 1 [
Hmox1], and PHD (plant homeo domain) finger protein 16 [
Phf16]) were upregulated while two genes (G0/G1 switch gene 2 [
G0s2] and patatin‐like phospholipase domain containing 3 [
Pnpla3]) were downregulated by these four antiadipogenic compounds. Tissue expression profiles showed that the
G0s2 and
Pnpla3 expressions were highly specific to adipose depots while the other three induced genes were ubiquitously expressed with significantly higher expression in adipose tissues. While
Cxcl1 expression was decreased, expressions of the other four genes were significantly increased during adipogenic differentiation of C3H10T1/2 cells. Small interfering RNA–mediated knockdown including Phf16 and Pnpla3 indicated that these genes might play regulatory roles in lipid accumulation and adipocyte differentiation. Specifically, the silencing of two newly identified adipogenic genes, Phf16 or Pnpla3, suppressed lipid accumulation and expression of adipocyte markers in both 3T3‐L1 and C3H10T1/2 cells. Taken together, these data showed previously uncovered roles of Phf16 and Pnpla3 in adipogenesis, highlighting the potential of using phytochemicals for further investigation of adipocyte biology.
A novel splice‐site mutation in the ATP2C1 gene of a Chinese family with Hailey‐Hailey disease
Authors: Xiao Heng , Huang Xiangjun , Xu Hongbo , Chen Xiang , Xiong Wei , Yang Zhijian , Deng Xiong , He Zhenghao , Deng Hao 
Published: September 2018

AbstractHailey‐Hailey disease (HHD), also known as familial benign chronic pemphigus, is an autosomal dominant genodermatosis. It is characterized by erosions, blisters and erythematous plaques at sites of friction or intertriginous areas. The pathogenic gene of HHD has been revealed as the ATPase secretory pathway Ca2+ transporting 1 gene (
ATP2C1), which encodes the protein, secretory pathway Ca
2+/Mn
2+‐ATPase 1 (SPCA1).
ATP2C1 gene mutations are responsible for HHD by resulting in abnormal Ca
2+ homeostasis in the skin and giving rise to acantholysis, a characteristic pathology of HHD. In this study, a four‐generation family containing three HHD sufferers was recruited. Direct sequencing of the
ATP2C1 gene was performed in the proband and other available family members. Reverse‐transcriptase polymerase chain reaction analysis was conducted to show the potential variant effect on
ATP2C1 splicing. A novel heterozygous c.325‐2A>G transition at the splice acceptor site of intron 4 in the
ATP2C1 gene was identified, and it co‐segregated with the disease in this family. The mutation resulted in exon 5 skipping and an in‐frame deletion of 12 amino acids (p.Ala109_Gln120del) in SPCA1. This splice‐site mutation may be responsible for HHD in this family. This study would further expand the mutation spectrum of the
ATP2C1 gene and may be helpful in the genetic counseling and prenatal diagnosis of HHD.
Prognostic role of TPD52 in acute myeloid leukemia: A retrospective multicohort analysis
Authors: Ha Mihyang , Han Myoung‐Eun , Kim Ji‐Young , Jeong Dae Cheon , Oh Sae‐Ock , Kim Yun Hak 
Published: September 2018

AbstractGenerating accurate prognoses is extremely important for treating patients with cancer. Prognostic prediction based on messenger RNA (mRNA) expression has shown superior clinical value to other markers for some cancers but is not currently used for acute myeloid leukemia (AML). Lipid metabolism is associated with biological aspects of cancer progression, including massive proliferation, and abnormal signaling. Moreover, abnormalities in lipid metabolism have prognostic significance. Patients with AML display abnormalities in sphingolipid metabolism and fatty acid oxidation. TPD52 is a regulator of lipid metabolism and plays a role in the formation of lipid droplets and fatty acid storage. Although the prognostic significance of
TPD52 expression has been reported for many types of cancer, it has not yet been assessed in patients with AML. Therefore, the aim of the current study was to assess the prognostic significance of
TPD52 in AML using three independent AML cohorts: one from The Cancer Genome Atlas (TGCA; n = 142) and two from the National Center for Biotechnology Information: GSE12417 (GPL96‐97; n = 162) and GSE12417 (GPL570; n = 78).
TPD52 was found to be overexpressed in patients with AML (GSE84881; n = 23). The Kaplan‐Meier curve revealed that
TPD52 overexpression was associated with a poor prognosis for patients with AML with good discrimination (
P = 0.013,
P = 0.005, and
P = 0.032 for the TGCA, GSE12417, and GSE12417, respectively). Analysis of C‐indices and area under the receiver operating characteristic curve values further supported this discriminative ability. Moreover, multivariate analysis confirmed the prognostic significance of
TPD52 expression levels (
P = 0.0196). These results suggest that the
TPD52 mRNA level is a potential biomarker for AML.
Sense of place attitudes and quality of life outcomes among African residents in a multicultural Australian society
Authors: Counted Victor 
Published: September 2018

AbstractThe study examines the association between sense of place (SOP) attitudes (e.g., place attachment, place identity, and place dependence) and health‐related quality of life (HQOL) in a sample of 261 African residents in New South Wales (Australia). Participants completed measures of the Sense of Place Scale, the World Health Organization Quality of Life‐BREF questionnaire, and demographic variables. Study findings are as follows: (a) levels of SOP and place attachment are positively associated with all outcomes of HQOL; (b) place identity is also positively related to HQOL in terms of better environmental health, psychological health, and physical health, but not statistically significant for social relationship and general QOL; (c) place dependence is statistically associated with outcomes of HQOL, except for general QOL which remained insignificant in both the unadjusted and adjusted models. Further ad hoc analyses suggest that African residents from Eastern Africa are more likely to develop the “aussie” place identity than those from Central Africa; and (e) African migrants who have been residents of Australia for more than 5 years, and those who are less educated are more likely to have a stronger SOP and to develop place attachment and behavioral place commitments compared with newly arrived and educated migrants, respectively. Study limitations and implications are carefully discussed.
Birds of a feather: Is matching based on shared interests and characteristics associated with longer youth mentoring relationships?
Authors: Raposa Elizabeth B. , Ben‐Eliyahu Adar , Olsho Lauren E.W. , Rhodes Jean 
Published: September 2018

AbstractYouth mentoring practitioners and researchers have shown a growing interest in determining the ways in which mentor–youth matching practices might influence the duration and effectiveness of mentoring relationships. The current project tested whether mentor–youth similarities at baseline, in terms of demographic variables and interests in certain activities (e.g., sports, art), predicted a longer duration of mentoring relationships. Analyses used baseline and follow‐up data from over 9,000 youth who participated in community‐based mentoring programs in the northeastern United States, as well as their volunteer mentors. Racial and ethnic similarity between mentor and youth was predictive of longer match duration. Moreover, a shared dislike of activities was associated with longer matches than either shared interests or discordant interests in activities. Findings have important implications for determining the ways in which mentor–youth matching practices influence the length and effectiveness of mentoring relationships.
LncRNA DSCAM‐AS1 acts as a sponge of miR‐137 to enhance Tamoxifen resistance in breast cancer
Authors: Ma Yun , Bu Deyong , Long Jiang , Chai Wenying , Dong Jian 
Published: September 2018

AbstractObjectiveTo investigate the influence of long noncoding RNA (lncRNA) DSCAM‐AS1 on the propagation and apoptosis of Tamoxifen‐resistant (TR) breast cancer cells via regulation of mircoRNA (miR)‐137 and epidermal growth factor receptor pathway substrate 8 (EPS8).MethodsData of GSE5840 downloaded from the Gene Expression Omnibus database were utilized to screen out aberrantly expressed lncRNA and messenger RNA in breast cancer tissue samples. The expressions of DSCAM‐AS1, miR‐137, and EPS8 were determined by quantitative real time polymerase chain reaction (qRT‐PCR). Cell lines were screened by half maximal inhibitory concentration (IC
50). 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide (MTT) assay and the flow cytometry assay were used to detect cell proliferation, apoptosis, and cell cycle. The relationship among DSCAM‐AS1, miR‐137, and EPS8 was studied by miRcode, TargetScan, and Pearson correlation coefficient. A xenograft mouse model experiment was performed to demonstrate the effect of DSCAM‐AS1 and EPS8 on tumor growth in vivo.ResultsLncRNA DSCAM‐AS1 and EPS8 were significantly upregulated, whereas miR‐137 was downregulated in TR tissues. DSCAM‐AS1 could promote the Tamoxifen resistance of breast cancer, and it was negatively correlated with miR‐137, whereas positively correlated with the expression of EPS8 in TR breast cancer tissues. Furthermore, miR‐137 could inhibit tumor development and arrest cell cycle at the G0/G1 phase by targeting the 3′‐UTR of EPS8. DSCAM‐AS1 targeted miR‐137 and EPS8 to promote propagation of TR breast cancer cells and inhibit cell apoptosis.ConclusionLncRNA DSCAM‐AS1 acts as a competing endogenous RNA of miR‐137 and regulates EPS8 to promote cell reproduction and suppresses cell apoptosis in TR breast cancer.
Effect of autophagy gene DRAM on proliferation, cell cycle, apoptosis, and autophagy of osteoblast in osteoporosis rats
Authors: Tang Ning , Zhao Hong , Zhang Hengyan , Dong Yulei 
Published: September 2018

AbstractObjectiveThe research aimed at detecting the autophagy level of osteoblast in osteoporosis rat, and investigating the effect of autophagy gene damage‐regulated autophagy modulator (DRAM) on osteoblast proliferation, cell cycle, apoptosis, and autophagy.MethodsThe level of osteocalcin (OCN) and C‐telopeptide (CTX) in serum of ovariectomized (OVX) rats was detected by enzyme‐linked immunosorbent assay (ELISA). The Oil Red‐O staining was used to observing bone histological changes. The messenger RNA level and protein expression level of Runt‐related transcription factor 2 (Runx2; osteoblast markers) and other autophagy‐related genes were revealed using quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blot. The changes of autophagy in osteoblasts were detected by immunofluorescence staining. The following experiments were performed in osteoblasts of OVX rats through transfected with silencing
DRAM to detecting cell proliferation, cell cycle, and apoptosis by Cell Counting Kit‐8 assays and flow cytometry.ResultsThe result of ELISA showed a significantly elevated of OCN and CTX in OVX rats as well a high fat content compared with sham‐operated rats. The expression of Runx2 in bone of proximal tibia was higher by qRT‐PCR and western blot analysis. The immunofluorescence staining and transmission electron microscope observe revealed that pcDNA3‐DRAM could promote the autophagy in OVX rats. Besides that, overexpression of
DRAM inhibited cell proliferation, promoted apoptosis, and enhanced autophagy in osteoblasts. The results of Oil Red‐O staining indicated that overexpression of DRAM enhanced lipid accumulation in osteoporosis rats.ConclusionsThe autophagy level of OVX rats was weakened, but overexpressed DRAM could increase the autophagy level of osteoblast, suppress proliferation, and induce apoptosis of osteoblast.
Fibronectin‐1 modulated by the long noncoding RNA OIP5‐AS1/miR‐200b‐3p axis contributes to doxorubicin resistance of osteosarcoma cells
Authors: Kun‐Peng Zhu , Chun‐Lin Zhang , Xiao‐Long Ma , Lei Zhang 
Published: September 2018

AbstractChemoresistance has been an obstacle in the further improvement of 5‐year survival rates of osteosarcoma (OS) patients, but the underlying mechanism of chemo‐resistance remains unclear. A comprehensive analysis of mRNAs and noncoding RNAs related to OS chemo‐resistance could help solve this problem. In the current study, we first identified that fibronectin‐1 (FN1), screened by microarray analysis in three paired chemo‐resistant and chemo‐sensitive OS cell lines, was significantly upregulated in the chemo‐resistant OS cell lines and tissues and was related to unfavourable prognosis. Further functional assays revealed that FN1 inhibition greatly increased the sensitivity of OS cells to doxorubicin in vitro and in vivo, whereas FN1 overexpression had the opposite effect. Moreover, mechanistic investigation demonstrated, by a series of assays that included luciferase reporter gene, RNA immunoprecipitation, RNA pull‐down and rescue assays, that FN1 expression was regulated by the oncogenic long noncoding RNA (lncRNA) OIP5‐AS1 through sponging miR‐200b‐3p. Thus, these results indicated the role and potential application of the lncRNA OIP5‐AS1/miR‐200b‐3p/FN1 regulatory pathway as a promising target in treatment of OS chemo‐resistance.
Acute changes in cardiac function by direct acting antiviral therapy for hepatitis C−infected patients with thalassemia
Authors: Khosravi Arezoo , Karimi‐Sari Hamidreza , Abedi‐Andani Mahdi , Behnava Bita , Namvar Ali , Anvar Ali , Alavian Seyed Moayed 
Published: September 2018

BackgroundPatients with thalassemia may also have cardiac abnormalities due to congenital problems, anemia, and increased burden of iron in their myocardium. This study was designed to evaluate the effects of direct acting antiviral (DAA) therapy on the cardiac function of hepatitis C virus (HCV)–infected patients with thalassemia.MethodHCV−infected thalassemia patients were enrolled to this prospective evaluation. Daily tablets of 90 mg Ledipasvir (or 60 mg Daclatasvir) plus 400 mg Sofosbuvir (±ribavirin) were prescribed for the patients according to the Iran Hepatitis Network's guidelines. An echocardiography fellow collected the echocardiography findings before and after the treatment of all the patients. The patients were followed up for any cardiac events within 12 weeks after finishing the treatment.ResultsThirty‐two patients with the mean age of 24.2 ± 6.4 years were evaluated. All patients showed a sustained virological response at the 12th week after finishing the treatment. The patients’ left ventricular end systolic diameter (3.0 vs 3.24; P = 0.003) and volume (33.8 vs 43.6; P = 0.001), global longitudinal strain of the left ventricle (−22.0 vs −20.6, P = 0.046), and average (−21.4 vs −20.3; P = 0.048), and the right ventricle size (3.12 vs 3.31; P = 0.012) were significantly increased after finishing the treatment. Changes in the abovementioned parameters were not correlated with the patients’ myocardium iron load. There were no significant differences in other echocardiographic parameters (
P > 0.05) before and after the treatment.ConclusionSofosbuvir‐based regimens for HCV treatment were safe for our HCV−infected patients with thalassemia. Our patients’ ejection fraction remained unchanged. Hence, more specialized echocardiographic evaluations were recommended for those with a history of cardiac abnormalities, cardiac iron overload, and in case of any cardiac adverse event during DAA therapy in patients with thalassemia.
Alternative food matrices for snack formulations in terms of acrylamide formation and mitigation
Authors: Mesías Marta , Delgado‐Andrade Cristina , Morales Francisco J 
Published: September 2018

AbstractBACKGROUNDThe salty snack market is re‐inventing itself trying to offer to consumers healthier and added‐value products often based on alternative vegetables/cereals, such as vegetable chips. New options mostly involve a nutritional improvement but the toxicological aspects of the reformulation are not usually examined. This work evaluated the acrylamide formation and effectiveness of different mitigation strategies in potato chips and in alternative snacks, such as carrot and pumpkin chips. Fresh potato, carrot and pumpkin samples were fried under controlled conditions and after being subjected to different operations (soaking in water at different times/temperatures, adding lemon or vinegar as pH modifiers or salt).RESULTSAcrylamide values in control samples of carrot (224 ± 47 µg kg−1) and pumpkin (514 ± 83 µg kg−1) were significantly lower than in the potato chip samples (3887 ± 509 µg kg−1). The different mitigation treatments had effects on potato and pumpkin chips, the most effective strategies being soaking options and the decrease of the pH of the medium. However, such treatments were not as effective in carrot chips.CONCLUSIONSThese new vegetable snacks could be a healthy alternative both at nutritional and toxicological level. In addition, acrylamide concentration could even be reduced with simple mitigation operations before frying. © 2018 Society of Chemical Industry
How Valid Is Tomographic Ultrasound Imaging in Diagnosing Levator and Anal Sphincter Trauma?
Authors: Turel Friyan , Shek Ka Lai , Dietz Hans Peter 
Published: September 2018

ObjectivesThree‐/four‐dimensional translabial ultrasound (US) is increasingly used to image the levator ani and anal sphincters, especially in the form of tomographic US. The aim of the study was to evaluate the validity of these published methods.MethodsThis work was a retrospective analysis of datasets of 172 nulliparous women who attended 2 tertiary urogynecologic centers for symptoms of pelvic floor dysfunction between June 2012 and September 2016. All patients had a standardized interview, clinical examination, and 4‐dimensional translabial US examination. An evaluation of volume data was performed by the first author, who was blinded against all clinical data, including parity. An assessment for levator avulsion and anal sphincter defects was performed using stored US volume data.ResultsData sets of 162 and 153 nulliparous women were available for levator and anal sphincter assessments, respectively. On the assessment by the first author, a complete avulsion was diagnosed in 3 nulliparas. On a review by 2 senior authors, 1 was judged as positive and the other 2 as normal. On the sphincter assessment, 2 significant external anal sphincter defects were detected. On the review, 1 was judged as false‐positive. The second was judged as highly abnormal by all authors. Therefore, 1 nullipara each was diagnosed with avulsion and a significant external anal sphincter defect.ConclusionsPublished diagnostic criteria for levator avulsion and external anal sphincter trauma on tomographic US imaging are highly unlikely to result in false‐positive findings. This finding supports the clinical validity of this method.
Impact of Fabp1 Gene Ablation on Uptake and Degradation of Endocannabinoids in Mouse Hepatocytes
Authors: McIntosh Avery L. , Huang Huan , Landrock Danilo , Martin Gregory G. , Li Shengrong , Kier Ann B. , Schroeder Friedhelm 
Published: September 2018

AbstractLiver fatty‐acid‐binding protein (FABP1, L‐FABP) is the major cytosolic binding/chaperone protein for both precursor arachidonic acid (ARA) and the endocannabinoid (EC) products N‐arachidonoylethanolamine (AEA) and 2‐arachidonoylglycerol (2‐AG). Although FABP1 regulates hepatic uptake and metabolism of ARA, almost nothing is known regarding FABP1’s impact on AEA and 2‐AG uptake, intracellular distribution, and targeting of AEA and 2‐AG to degradative hepatic enzymes. In vitro assays revealed that FABP1 considerably enhanced monoacylglycerol lipase hydrolysis of 2‐AG but only modestly enhanced AEA hydrolysis by fatty‐acid amide hydrolase. Conversely, liquid chromatography–mass spectrometry of lipids from Fabp1 gene‐ablated (LKO) hepatocytes confirmed that loss of FABP1 markedly diminished hydrolysis of 2‐AG. Furthermore, the real‐time imaging of novel fluorescent NBD‐labeled probes (NBD‐AEA, NBD‐2‐AG, and NBD‐ARA) resolved FABP1’s impact on uptake vs intracellular targeting/hydrolysis. FABP1 bound NBD‐ARA with 2:1 stoichiometry analogous to ARA, but bound NBD‐2‐AG and NBD‐AEA with 1:1 stoichiometry—apparently at different sites in FABP1’s binding cavity. All three probes were taken up, but NBD‐2‐AG and NBD‐AEA were targeted to lipid droplets. LKO reduced the uptake of NBD‐ARA as expected, significantly enhanced that of NBD‐AEA, but had little effect on NBD‐2‐AG. These data indicated that FABP1 impacts hepatocyte EC levels by binding EC and differentially impacts their intracellular hydrolysis (2‐AG) and uptake (AEA).
Uncertainties of calculated Cramér‐Rao lower bounds: implications for quantitative MRS
Authors: Bonny Jean‐Marie , Pagès Guilhem 
Published: September 2018

PurposeTo investigate the uncertainties on relative Cramér‐Rao lower bound (rCRB) estimates and demonstrate their biasing effects in MRS quantification.Theory and MethodsSimulations were performed to calculate the distribution of the computed rCRB (noted rCRB*) for several rCRB levels. One hundred thousand simulations per rCRB value were performed on simulated NMR signals containing either 1 signal (singlet) or 2 partially overlapping signals. False‐positive and false‐negative risks were compiled for different threshold levels. rCRB* distribution was experimentally checked on a deuterated water sample using a 9.4 Tesla vertical magnet.ResultsSimulations showed that (1) rCRB* distribution is asymmetrical, with a right‐tailed distribution increasing the risk of accepting false‐positive data (i.e., accepting an rCRB* value whereas the true rCRB value is higher than the threshold level) and (2) distribution broadness increases with increasing rCRB level. Simulations with overlapped peaks lead to more inaccuracy in the rCRB estimation. Probabilities of false detection increase with increasing threshold level.ConclusionAnalyzing results thanks to a CRB (or rCRB) based on experimental measurements might lead to misinterpretations. To exploit the rCRB* as rejection criteria, a conservative threshold level of 20% is recommended in order to limit the probability of false alarms.
ICS educational module: Cystometry in children
Authors: Wen Jian G. , Djurhuus Jens Christian , Rosier Peter F.W.M. , Bauer Stuart B. 
Published: September 2018

AimsTo introduce the standard procedure of cystometry and interpretation of the results in children.MethodsThe literature on cystometry in children in PubMed for the last 20 years was reviewed. The updated knowledge regarding indication, preparation, technique, and interpretation of cystometry in children were summarized.ResultsFilling cystometry is the core content of a paediatric urodynamic study. In this section, the technique for performing cystometry is introduced in details. Emphasis is placed on correctly setting up the equipment according to ICS and ICCS guidelines, using appropriate terminology, providing indications for its performance with specific considerations for children, and proper interpretation of results.ConclusionsCystometry can be used in children including newborn to evaluate lower urinary tract dysfunction.