bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neural evidence for action-related somatosensory predictions
Authors: Moran Caoimhe , Hogendoorn Hinze , Landau Ayelet N. 
Published: November 2024

AbstractThe tactile consequences of self-initiated movements are thought to be predicted by a forward model, yet the precise neural implementation of these predictions remains unclear. In non- motor contexts, expectations are thought to activate sensory neurons tuned towards the expected stimulus. This acts as a predictive template against which afferent sensory input is compared. It is unclear whether forward model predictions have a similar neural instantiation. Here we employed time-resolved multivariate decoding on human electroencephalography (EEG) during self-generated movements to examine the content of predictive neural activity. Human participants performed index finger movements which were predictably paired with a vibration to either the index or ring finger of the opposite, passive hand. On some trials the tactile stimulus was unexpectedly omitted. Results revealed above-chance finger decoding in the pre-movement period supporting a predictive representation of expected stimulation location. As the movement approached, this predictive activity became similar to late-stage processing of a physical tactile stimulus. On omission trials, we found that despite the absence of afferent input, finger location could be decoded ∼120 ms after expected stimulus onset. This shows a stimulus-specific omission response. Together these findings indicate that self-generated movement pre-activates neurons tuned towards expected tactile consequences.
Semi-automated navigation for efficient targeting of electron tomography to regions of interest in volume correlative light and electron microscopy
Authors: Konishi Kohki , Neves Guilherme , Russell Matthew , Mimura Masafumi , Burrone Juan , Fleck Roland 
Published: November 2024

AbstractElectron microscopy is essential for the quantitative study of synaptic ultrastructure. At present, the correlation of functional and structural properties of the same synapse is extremely challenging. We introduce a novel integrated workflow designed to simplify sample navigation across spatial scales, allowing the identification of individual synapses from optical microscopy mouse brain image stacks that can be targeted for analysis using electron tomography imaging. We developed a software which has a function to register multimodal images using a novel segmentation-based image registration algorithm as well as a function to visualize all the registration results. Using our newly designed software we streamline mapping of high-resolution optical imaging onto reference maps using blood vessels as endogenous fiducial marks. Further we demonstrate significant improvements on the ultramicrotomy stage of volume Correlative Light and Electron Microscopy (vCLEM) workflows, providing real time guidance to targeted trimming to match previously acquired Regions Of Interest (ROIs), and reliable estimates of cutting depth relative to ROI, based on fluorescence imaging of TEM ready ultrathin sections. Using this workflow, we successfully targeted TEM tomography to the proximal axonal region containing the Axon Initial Segment identified using fluorescent light microscopy.
Revealing the Complexity of the Epicardial Secretome: Characterization and Functional Assessment of Epicardium-Derived Extracellular Vesicles and Matrix
Authors: Oliveira Cláudia C. , Córdoba José , Pearson John R. , Guruceaga Elizabeth , Marín-Sedeño Ernesto , García-Caballero Melissa , Guadix Juan Antonio , Pérez-Pomares José M. , Ruiz-Villalba Adrián 
Published: November 2024

AbstractAimThe epicardium, an epithelial layer covering the heart, plays pivotal roles in embryonic heart development and responses to cardiac damage. Epicardial secreted molecular agents are known to be involved in the regulation of these phenomena, but how this regulation occurs is poorly understood. In this study, we have investigated extracellular vesicle (EV) and extracellular matrix (ECM) components of epicardial secretions using a continuous mouse embryonic epicardial-derived cell (EPIC) line.Methods and resultsEpicardial-derived EVs were isolated using differential ultracentrifugation from EPIC cultured at 1% (EVs-H1%), 5% (EVs-H5%), and 21% oxygen (EVs-N). EVs protein content was determined by tandem mass tag (TMT) proteomic analysis. The results showed that epicardial-derived EVs cargo is sensitive to the oxygen level of their parenteral cells, increasing their content on glycolytic proteins. Moreover, hypoxic-derived EVs were found to both increase EPIC proliferation and affect the metabolism of Primary Human Umbilical Vein Endothelial Cells (HUVECs).On the other hand, epicardial-derived extracellular matrix (EPIC-ECM) was characterized by subjecting decellularized EPIC to shotgun proteomics and comparing it to decellularized perinatal hearts and Matrigel®. We found that EPIC-ECM composition closely resembles that of embryonic cardiac tissue. Although the structural and basement membrane-associated proteins of EPIC-ECM were similar to those found in Matrigel®®, EPIC-ECM exhibited higher protein diversity and was a more potent inducer of HUVEC proliferation.ConclusionThis work represents the first comprehensive and systematic proteomic analysis of two important components of the epicardial-derived secretome. Our experiments reveal that the epicardium responds to hypoxia by secreting EVs capable of modifying the metabolic responses of surrounding cells. Furthermore, EPIC-ECM promotes endothelial cell proliferation. These findings demonstrate the significant signaling abilities of the epicardial secretome, consistent with reports of endothelial responses following cardiac ischemic damage.
4-Methyllumifrone (4-MU) can improve learning and memory after cerebral ischemia/reperfusion injury in rats
Authors: Tamouk Shaghayegh , Jahangiri Hamzeh Mirshekari , Jahromi Elham Kashafi , Hamblin Michael R , Ramezani Fatemeh , Aboutaleb Nahid 
Published: November 2024

AbstractBackgroundStroke is the sixth leading cause of death and lifelong disability for millions of people in the United States. Cerebral ischemia leads to oxidative stress, excitotoxicity, inflammation, apoptosis; additionally, impairments in memory and learning occur in the majority of subjects with ischemic stroke. The lack of definitive treatment has sparked extensive research into novel therapeutic strategies, including the use of 4-methylumbelliferone (4-MU), a coumarin derivative with potential neuroprotective properties. The present study examines the impact of 4-MU on reducing cerebral ischemia-reperfusion (I/R) injury and learning and memory impairments in male Wistar rats.MethodThe animals were exposed to middle cerebral artery occlusion (MCAO) and were treated with one dose of 4-MU (at a dosage of 25 mg/kg) dissolved in DMSO 0.9%. Automated shuttle box and Morris water maze (MWM) test were employed to evaluate learning and memory impairments. Western blot assay, TTC staining and Nissl staining were used to measure protein expression, infarct volume, and cell death, respectively.ResultsResults showed that treatment with 4-MU reduced infarct volume and improved learning and memory impairments by down-regulating HAS1 and HAS2. 4-MU has been shown to modulate the release of pro-inflammatory cytokines including TNF-α and IL-1β, as well as anti-inflammatory markers like IL-10 and also reduced oxidative stress markers in the brain.ConclusionGenerally, the neuroprotective effects of 4-MU against cerebral I/R injury can be attributed to the down-regulation of HAS1 and HAS2.
Melanophore and fluoroleucophore synergistically photo-protect the Arabian killifish,Aphanius dispar, embryo from ultraviolet light
Authors: Alenize Maryam , Minhas Rashid , Kudoh Tetsuhiro 
Published: November 2024

AbstractPigment cells in fish species play crucial roles in forming colour patterns of each species and other physiological characteristics including photoprotection. Research on photoprotection by pigment cells in animals has primarily concentrated on black pigment cells, known as melanophores. However, the roles of other pigment cells and their synergistic effects on UV protection remain poorly understood. In this study, we use the Arabian killifish embryos as a model for studying the mechanisms of UV protection by different pigment cells. This species features highly fluorescent pigment cells called fluoroleucophores and black pigment cells known as melanophores. The fluorescent pigments and black melanin pigments are generated by genesgch(GTP cyclohydrolase) andtyr(tyrosinase) respectively. We generatedgch(−/−)andgch/tyr(−/−)double mutant lines using CRISPR/Cas9 genome editing and examined the UV sensitivity of these mutant embryos. Both morphology and gene expression data revealed that thegch/tyr(−/−)double mutant line exhibited the highest UV sensitivity, and thegch(−/−)line also demonstrated a greater stress response compared to wild type (WT). From the study, we have identified the synergistic role of black and fluorescent pigment cells in providing effective UV protection from the early stages of embryonic development.
Navigating through processes: a bibliometric landscape of BPM in human resources
Authors: Salomão David Maria Laura , Dallavalle Silvia , Eustachio João Henrique Paulino Pires , Lourenção Marina 
Published: November 2024

PurposeThis study aims to bridge the gap in the existing literature by exploring BPM practices applied to HRM to optimize organizational performance, identifying key thematic clusters and highlighting potential areas for future research.Design/methodology/approachAfter applying the inclusion and exclusion criteria, 177 papers on BPM practices in HRM were selected. This sample was used for a bibliometric assessment based on the co-occurrence of terms technique. We also described key studies on BPM in HRM.FindingsThe analysis identified five key theoretical clusters, highlighted the benefits of BPM in enhancing HRM, such as improved leadership, knowledge management, sustainability, performance and talent management and noted challenges in BPM implementation.Originality/valueBy synthesizing existing literature, this research enriches scholarly understanding and offers practical insights for HR practitioners aiming to optimize organizational performance. The findings emphasize the importance of considering human-centric perspectives in BPM and provide practical guidance for HR professionals aiming to enhance organizational performance through improved BPM practices.
Making knowledge management transparent: a new perspective on KM processes integration in the organizational framework
Authors: Nakash Maayan , Bolisani Ettore 
Published: November 2024

PurposeWhile scholars agree on the complexity of knowledge management processes (KMPs), achieving a comprehensive understanding of these processes and how they integrate into the core organizational processes still proves challenging. In response to this research gap, this study presents a pioneering, inductive exploration into the views of chief knowledge officers (CKOs).Design/methodology/approachWe employed an interpretative multiple-case study methodology, conducting semi-structured interviews with 28 CKOs across diverse industry sectors. Thematic analysis was utilized to analyze the data.FindingsThe CKOs’ insights reveal that the degree of “superposition and overlap” between KMPs and business processes is pivotal to the effectiveness of both knowledge management (KM) and business operations. The study introduces the concept of “transparent KM,” where KM becomes an imperceptible yet indispensable element of the organizational structure. This challenges the conventional notion of KM as an isolated discipline and highlights the dangers of artificially segregating KMPs from core business processes, which can result in employee disengagement and managerial doubts about the value of KM.Originality/valueThis research contributes to a novel vision of KM, advocating for the seamless integration of KMPs into organizational processes. It suggests that KM should be considered a ubiquitous force that naturally enhances organizational workflows. Scholars, practitioners and company leaders, in designing business processes, should take into consideration their KM implications and, in turn, potentially reshaping the strategic role of KM in organizations.
Beyond the headlines: understanding the organizational dynamics of sector-specific sexual harassment
Authors: Nuñez Imanol , Ollo-López Andrea 
Published: November 2024

PurposeSexual harassment, a global concern, varies in prevalence across sectors. This article analyzes in detail the higher prevalence of harassment in these sectors.Design/methodology/approachDrawing on data from a representative sample of European workers and using the inductive method, we posit four propositions regarding the organizational dynamics in four different sector-specific scenarios.FindingsContrary to perception, our research finds no conclusive evidence supporting a higher prevalence of sexual harassment within the armed forces. Economic and labor conditions drive sexual harassment in the entertainment industry, while the hospitality and non-profit sectors exhibit an elevated incidence potentially attributed to environmental factors. The healthcare industry presents a concerning scenario, with heightened prevalence due solely to organizational.Originality/valueThe originality of the paper is in its focus on how external and environmental factors, rather than just internal organizational mechanisms, contribute to sector-specific dynamics of sexual harassment. This approach provides deeper insights for developing tailored interventions and prevention strategies across different sectors.
State-owned equity participation and greenwashing behavior: evidence from Chinese private enterprises
Authors: Ren Guangqian , Jing Man , Liu Li , Zheng Minna 
Published: November 2024

PurposeCan state-owned equity participation inhibit private enterprises’ greenwashing behavior? If so, what are the mechanisms involved? Is there any difference in the impact of state-owned equity participation on private enterprises’ greenwashing behavior in different contexts? The answers to the above questions not only fill the existing research gaps but also provide new research ideas for greenwashing governance in developing countries.Design/methodology/approachUsing a sample of Chinese A-share listed private enterprises from 2011 to 2022, we examine the impact of state-owned equity participation on private enterprises’ greenwashing behavior.FindingsThe results suggest that state-owned equity participation can significantly inhibit private enterprises’ greenwashing behavior. Mechanism analysis shows that state-owned equity participation suppresses private enterprises’ greenwashing behavior by alleviating financing constraints through the resource effect and reducing managerial myopia through the governance effect. Moderating effect analysis indicates that media attention can strengthen the inhibitory effect of state-owned equity participation on private enterprises’ greenwashing behavior. Heterogeneity analysis shows that the inhibitory effect of state-owned equity participation on private enterprises’ greenwashing behavior is more significant in areas with a low degree of marketization and non-heavy-polluting industries.Originality/valueThe findings enrich the governance factors of private enterprises’ greenwashing conduct from the standpoint of diverse shareholders, assist developing countries in formulating more specific policy goals and provide important insights into global environmental governance practices.
Global work–life balance trailblazing: how to lead the world in work–life balance, with insights from Denmark
Authors: Warren Tracey , Hyltoft Lene 
Published: November 2024

PurposeThe article asks how to lead the world in work–life balance (WLB) societal rankings, identifies the major WLB challenges in contemporary societies and rethinks how we conceptualise and measure WLB.Design/methodology/approachThis study employed a case-study of Denmark, a world leader in global WLB-rankings. Expert-insider interviews were carried out with 12 experts who work in the field of WLB. The participants were engaged day-to-day in the field of interest, exerting real-world influence.FindingsWe affirm the importance for WLB of battling over-long hours in the workplace. We provide evidence too that having sufficient hours of work, and being financially secure, are as significant for WLB as is avoiding overwork.Originality/valueThe article interrogates international WLB-rankings and offers original insights into a global WLB-leader. It makes the case for improving how we conceptualise and measure WLB to also progress policies and practice. Rather than viewing WLB as largely about avoiding a time-squeeze, the article argues that it is fundamentally about the conditions under which people work.
Consumer resistance to sustainable fashion: evidence from the USA and India
Authors: Busalim Abdelsalam , Lynn Theo , Wood Charles M. 
Published: November 2024

PurposeDespite increasing awareness among fashion consumers about the positive environmental and societal impacts of sustainable fashion as a viable alternative to fast fashion, their actual adoption behavior often diverges. This study aims to empirically investigate consumers’ resistance barriers to sustainable fashion clothing.Design/methodology/approachThis study utilizes innovation resistance theory to examine the barriers to consumer intention to buy sustainable clothing. The study collected a large sample (N = 745) of fashion consumers from the USA and India to test a research model.FindingsThe study finds that value, social risk, tradition and image barriers significantly reduce consumers’ intentions to buy sustainable fashion clothing. Additionally, the findings highlight that environmental concern moderates the relationship between social risk barriers and buying intentions.Originality/valueThe study findings contribute to the existing sustainable fashion literature by highlighting the main barriers for sustainable clothing consumption and emphasizing the crucial role of social elements, economic values and the image of sustainable fashion products in shaping consumer behavior within the fashion landscape.
Get cold feet: job hopping a conundrum in the stable employment of Gen Z employees at Industry 5.0
Authors: Chand Pawan Kumar , Tandon Urvashi , Mishra Neha 
Published: November 2024

PurposeThe present research study aims to understand the cause-and-effect reasons behind the job-hopping practices followed by Gen Z employees in the industry 5.0 in India. Further, in the tandem of efforts, the research study has examined the direct and indirect relationship among novice behaviour, social alienation and job-hopping in Gen Z in the information technology sector of Industry 5.0 in India.Design/methodology/approachThe 533 Gen Z or millennial employees were chosen from northern India Industry 5.0 following the non-probability purposive sampling technique. The study follows the quantitative research approach, and the data were collected through a survey questionnaire based on standardized measuring instruments. Further, the gathered data were analysed using the structure equation modelling.FindingsThe study’s findings confer the significant direct impact of novice behaviour on job-hopping. While measuring the indirect relationship, the partial mediation effect was noticed in the relationship among novice behaviour, social alienation and job-hopping in the Gen Z employees of Industry 5.0 in India.Originality/valueThe present study will be beneficial to the investors to recognize the job-hopping reasons in Industry 5.0. Further, Gen Z employees and academicians will also receive insight into the cause and effect behind job-hopping. Such will minimize the gap between industry and academia and help Gen Z attain stable employment in Industry 5.0.
Royal power in the market‐oriented society: The Swedish King's consecration of business and corporate elites
Authors: Holmqvist Mikael 
Published: November 2024

AbstractIn this paper, I examine how the King of Sweden, Carl XVI Gustaf, systematically consecrates the nation's business and corporate elites who have come to dominate Swedish society during the last decades concomitant with a fundamental transformation from traditional social‐democracy to neoliberalism, that is, a society characterized by the logic of corporations and markets. By promoting the business and corporate elites, the King contributes to strengthening their status and legitimacy in relation to other groups, while at the same time he reproduces his own elite status and image as a “corporate king.” In order to examine this dual elite legitimation, I have studied three major official duties in the King's official role as Sweden's head of state: (a) the awarding of the most prestigious royal medals to corporate leaders; (b) the invitation of these elites to official royal dinners; and (c) state visits, whereby the corporate elites are given a peculiar status in relation to other elite groups. Based on this unique data on the activities of a living monarch, I refute the common assumption among sociologists today that royals, and particularly monarchs, are powerless figures and therefore irrelevant as study objects. By consecrating business and its leaders, monarchs contribute to legitimizing neoliberalism, thus strengthening its hegemony, as well as their own standing. Hence, they are not only symbolic figures, but exercise real power as well.
Potential pesticide substrates of an insect ABCC transporter
Authors: Chen Jinli , Wang Dong , Liu Wei , Zhou Yuanyuan , Yang Qing 
Published: November 2024

AbstractThe use of synthetic pesticides carries a significant risk of pests developing resistance, leading to decreased pesticide effectiveness. ATP‐binding cassette (ABC) transporters, especially the ABCC subfamily members, have been suggested to act as efflux pumps for various pesticides, thereby contributing to pesticide resistance. So far, the identification of potential pesticide substrates of insect ABC transporters is most often based on the quantification of transcript in arthropods. Here, we screened and identified the potential pesticide substrates of ABCC‐9C from Tribolium castaneum based on an in vitro ATPase activity assay. Together with affinity evaluation‐, cytotoxicity analysis‐, and RNA interference‐based bioactivity tests, we revealed that the insecticides, carbofuran, and buprofezin, are potential substrates of TcABCC‐9C. Additionally, we identified an amphipathic translocation channel in the transmembrane domain of TcABCC‐9C formed by 8 transmembrane helices. Molecular docking suggested that both carbofuran and buprofezin bind at the same site within the translocation channel via hydrophobic interactions. These findings indicate that TcABCC‐9C might play a critical role in multi‐pesticide resistance, providing a potential target for managing pesticide resistance and laying the groundwork for future pest control strategies. Given the conservations among ABCC subfamily members, the experimental model we developed in this study can be also applied to identify the potential substrates of other ABCC transporters, as well as to predict insecticide resistance mediated by ABCC transporters.