bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-apoptotic function of T-KTS+, T-KTS-, WT1+/+ and WT1+/- isoforms in breast cancer
Authors: Graidist Potchanapond , Nawakhanitworakul Raphatphon , Saekoo Jiraporn , Dechsukhum Chavaboon , Fujise Ken 
Published: April 2018

Abstract
Background: WT1 was originally identified in Wilms tumor, a childhood kidney cancer. This gene was expressed in wide variety of solid cancers. Alternative splicing of WT1 transcript generates four major protein isoforms and thirty-six minor protein isoforms, each having different functional properties. WT1 gene has been considered as a tumor suppressor gene and anti-apoptotic protein. However, the mechanism of WT1 in breast cancer remains unclear. Objective: Evaluate the role of truncated WT1 isoforms (T-KTS+ and T-KTS-) and two major WT1 isoforms (+/+ and +/-) in apoptosis in breast cancer cell line, MCF-7. Materials and methods: RNA interference (RNAi) was employed in an attempt to define the role of WT1 in a breast cancer cell line (MCF-7). Furthermore, MCF-7 overe-xpressing cells that stably expressed two truncated WT1 isoforms (T-KTS+ and T-KTS-) or two major WT1 isoforms (+/+ and +/-) were generated and exposed to Doxorubicin. The mortality of cells was determined as a percentage of trypan blue-stained cells in total cells. The apoptotic molecules in apoptosis pathway were detected using RT-PCR, caspase-7 activity assay and Western blot analysis techniques. Results: Transfection of siRNAWT1 into MCF-7 cells resulted in decreasing of WT1 protein and related to the increasing in number of cell death and caspase-7 activity. Over-expression of T-KTS+, T-KTS-, WT1+/+ and WT1+/- isoforms protected cells from cell death induced by apoptosis-inducing agent, doxorubicin. Moreover, the expression of apoptotic p53, Bak and caspase-7 were decreased by the expression of all four WT1 isoforms, especially T-KTS- and T-KTS+ isoforms. Conclusion: T-KTS+ and T-KTS- isoforms as well as WT1+/+ and WT1+/- isoforms could function as an antiapoptotic protein in breast cancer cell line, MCF-7.
Preliminary serological and molecular investigation of selected viral pathogens in Croatian cervid species
Authors: Besi Roić , Svjetlana Terzić , Tihomir Florijančić , Jelena Prpić , Siniša Ozimec , Lorena Jemeršić , Ivica Bošković , Andreja Jungić , Tomislav Keros 
Published: April 2018

Abstract
A total of 131 blood samples and 175 spleen samples were collected from three cervid species: roe deer (Capreolus capreolus), red deer (Cervus elaphus) and fallow deer (Dama dama) inhabiting the continental part of Croatia. Serum samples were tested for antibodies against bovine herpesvirus 1, parainfluenza-3 virus, bluetongue virus, bovine respiratory syncytial virus, hepatitis E virus, bovine viral diarrhoea virus and enzootic bovine leukosis virus. The tested sera were negative for bovine viral diarrhoea virus, enzootic bovine leukosis virus, bluetongue virus, bovine respiratory syncytial virus and hepatitis E virus antybodies. The antibody prevalence in roe deer and red deer samples was 21.11% for bovine herpesvirus 1 and 75.55% for parainfluenza-3 virus. Sera from bovine herpesvirus 1 positive animals were subsequenty tested with comparative virus neutralization test and bovine herpesvirus 1 neutralising antibodies were found in 18 (out of 19) sera. In fallow deer, no antibodies against any of the viral pathogens were detected. All spleen samples tested for bovine viral diarrhoea virus and enzootic bovine leukosis virus came back negative, except for one red deer spleen sample found to be weakly diarrhoea virus-positive. Our findings provide the first information on the exposure of Croatia-inhabiting cervid species to viral pathogens, and could serve as valuable baseline data for future investigations regarding deer exposure to various pathogens and the distribution of diseases shared between wildlife and livestock. As of now, the epidemiology of these viruses in the Croatian cervid population has been only poorly understood, so that further research is recommended.
Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay
Authors: Giuseppa Purpari , Francesco Mira , Santina Di Bella , Simona Di Pietro , Elisabetta Giudice , Annalisa Guercio 
Published: April 2018

Abstract
Canine parvovirus type-2 (CPV-2) is a DNA virus that causes a very common worldwide diffused infectious disease in dogs. Since its appearance, the CPV-2 evolved generating novel genetic and antigenic variants (CPV-2a/2b/2c) which are distributed throughout the world. In the present study, the frequency of CPV-2 in a canine population in Sicily (Italy) was investigated, using a polymerase chain reaction (PCR) for a fragment of the VP2 gene. Out of a total of 673 samples from 370 dogs, submitted to the laboratory from July 2009 to August 2015, 265 samples (39.38%) were positive and these were further analyzed by restriction fragment length polymorphism (RFLP) and DNA sequence analysis. A high prevalence of the CPV-2c variant (79.56% of CPV-2 positive dogs) was observed in this survey, underlining that this type of CPV field isolate is prevalent circulating in Sicily. Sequence and phylogenetic analysis showed a close relationship with CPV-2a and CPV-2c strains from Europe and non-European countries. Considering that CPV-2c is reaching a worldwide distribution and that this variant is also affecting vaccinated dogs, efforts should be made towards the development of new CPV vaccines.
Involvement of serotonergic, noradrenergic and gabaergic systems in the antinociceptive effect of a ketamine-magnesium sulfate combination in acute pain
Authors: Katarina Savić Vujović , Sonja Vučković , Radan Stojanović , Nevena Divac , Branislava Medić , Dragana Srebro , Marko Kadija , Milica Prostran 
Published: April 2018

Abstract
Ketamine and magnesium can interact in additive, supra-additive and antagonistic manners in analgesia or anesthesia. Ketamine is a non-competitive NMDA receptor antagonist. Magnesium is an endogenous non-competitive NMDA antagonist that causes anion channel blockade in a dose-dependent manner. It has been established that ketamine and magnesium interact synergistically in the tail-immersion test in rats.
To determine the role of serotonergic, GABAergic and noradrenergic systems in analgesia induced by the ketamine-magnesium sulfate combination.
Experiments were performed on male Wistar albino rats (200-250 g). Antinociception was evaluated by the tail-immersion test.
Methysergide (0.5 and 1 mg/kg, sc) administered alone did not affect nociception in rats. Methysergide (0.5 and 1 mg/kg, sc) antagonized the antinociceptive effect of the ketamine (5 mg/kg)-magnesium sulfate (5mg/kg) combination. Bicuculline (0.5 and 1 mg/kg, sc) given alone did not change the threshold to thermal stimuli in rats. Bicuculline (0.5 and 1 mg/kg, sc) antagonized the antinociceptive effect of the ketamine (5 mg/kg)-magnesium sulfate (5 mg/kg) combination. Yohimbine (0.5, 1 and 3 mg/kg, sc) applied alone did not change nociception. Yohimbine at a dose of 0.5 mg/kg did not influence the effect of ketamine (5 mg/kg)-magnesium sulfate (5 mg/kg), while yohimbine at doses of 1 and 3 mg/kg antagonized the antinociceptive effect of this combination.
Serotonergic, noradrenergic and GABAergic systems participate, at least in part, in the antinociceptive effect of the ketamine-magnesium sulfate combination in acute pain in rats.
Civil service, 2017
Authors: Shannon Laura 
Published: April 2018

Abstract
With the election of a new Taoiseach, Leo Varadkar, TD, in June 2017, a number of changes were made to government departments. A new Department of Rural and Community Development was established, taking functions from the Department of Arts, Heritage, Regional, Rural and Gaeltacht Affairs (renamed the Department of Culture, Heritage and the Gaeltacht), and from the Department of Housing, Planning, Community and Local Government (renamed the Department of Housing, Planning and Local Government). The employment brief was moved to the Department of Social Protection to create the Department of Employment Affairs and Social Protection. Finally, the Department of Jobs, Enterprise and Innovation was renamed the Department of Business, Enterprise and Innovation.
European Union, 2017
Authors: Malone Margaret Mary 
Published: April 2018

Abstract
The year 2017 was eventful for the EU and its member states. Given the widespread Euroscepticism and populism which appeared to be on the rise last year, election results in the Netherlands, France and Germany were greeted with relief and hope for the future. The EU was in an optimistic mood. European Commission President Jean- Claude Juncker used his State of the European Union speech in September to note that the EU had the ‘wind in its sails’ (Juncker, 2017). At the same time, he cautioned that the fair weather conditions would not last long - there was no room for complacency. The EU had to act to protect, empower and defend its citizens. The EU moved forward on a number of policy fronts in the wake of the Brexit vote and also concluded high-profile international trade deals in an effort to fill the vacuum left by the protectionist policies of the Trump administration.
HPV Vaccination: Prevention of Cervical Cancer in Serbia and in Europe
Authors: Stark Holger , Živković Aleksandra 
Published: April 2018

Summary
The identification of the high-risk human papilloma viruses (HPVs) as a cause of cervical cancer offered the possibility for the development of a HPV vaccine. Twenty years after this identification of the HPV types, the first HPV vaccine came to the market. There are three HPV vaccines today on the market, all containing the virus-like particles (VLPs) of the HPV types 16 and 18, which are considered to cause 77 % of all cancers caused by HPVs. In addition, two of the vaccines contain two low-risk HPV types (6 and 11)-quadrivalent or the same as low-risk types and additional high-risk HPV types (31, 32, 45, 52, 58)-nonavalent vaccines. The cervical cancer protection efficacy of the vaccines is very high, around 100%. The VLPs of the 6- and 11-type offer efficient protection against genital warts. Unfortunately, the implementation of the vaccination is actually not so high despite all scientific and medical facts, but their rates in Europe are steadily increasing reaching about 90% in one country after another. In Serbia, all of the three vaccines are on the market but are highly underused. Actually, there is no national program and the Serbian vaccination rates are very low. High vaccination rates in Serbia need to be achieved as a goal of prevention of cervical cancer.
Clinical Conditions and Predictive Markers of Non-Dipper Profile in Hypertensive Patients
Authors: Tilea Ioan , Petra Dorina , Ardeleanu Elena , Hutanu Adina , Varga Andreea 
Published: April 2018

Abstract
Hypertension remains one of the primary causes of premature cardiovascular mortality representing a major independent risk factor.
The importance of ambulatory blood pressure monitoring in clinical evaluation of hypertensive patients, beyond diagnosis, is the identification of circadian dipping/non-dipping profile. The non-dipper pattern in hypertensive and normotensive patients is associated with significant target organ damage and worse outcomes, as an increased cardiovascular risk condition. Non-dipping pattern has been found to be associated with specific clinical conditions. Obesity, diabetes mellitus, metabolic syndrome, obstructive sleep apnea syndrome, chronic kidney disease, autonomic and baroreflex dysfunctions, salt sensitivity, hormonal changes, gender and age were extensively studied. Research efforts are focused on recognizing and exploring predictive markers of abnormal blood pressure circadian pattern. Previous studies acknowledge that red cell distribution width, mean platelet volume, fibrinogen level, C-reactive protein, serum uric acid and gamma-glutamyltransferase, are independently significant and positive associated to non-dipping pattern. Moreover, research on new biomarkers are conducted: Chitinase 3-Like-Protein 1, atrial and B-type natriuretic peptide, brain-derived neurotrophic factor, chemerin, sphingomyelin and the G972R polymorphism of the insulin receptor substrate-1 gene. This review summarizes the current knowledge of different clinical conditions and biomarkers associated with the non-dipper profile in hypertensive patients.
Kinematic-PPP using Single/Dual Frequency Observations from (GPS, GLONASS and GPS/GLONASS) Constellations for Hydrography
Authors: Farah Ashraf 
Published: April 2018

Abstract
Global Positioning System (GPS) technology is ideally suited for inshore and offshore positioning because of its high accuracy and the short observation time required for a position fix. Precise point positioning (PPP) is a technique used for position computation with a high accuracy using a single GNSS receiver. It relies on highly accurate satellite position and clock data that can be acquired from different sources such as the International GNSS Service (IGS). PPP precision varies based on positioning technique (static or kinematic), observations type (single or dual frequency) and the duration of observations among other factors. PPP offers comparable accuracy to differential GPS with safe in cost and time. For many years, PPP users depended on GPS (American system) which considered the solely reliable system. GLONASS's contribution in PPP techniques was limited due to fail in maintaining full constellation. Yet, GLONASS limited observations could be integrated into GPS-based PPP to improve availability and precision. As GLONASS reached its full constellation early 2013, there is a wide interest in PPP systems based on GLONASS only and independent of GPS. This paper investigates the performance of kinematic PPP solution for the hydrographic applications in the Nile river (Aswan, Egypt) based on GPS, GLONASS and GPS/GLONASS constellations. The study investigates also the effect of using two different observation types; single-frequency and dual frequency observations from the tested constellations.
A New Method for Measuring Angular Increments Based on a Tri-Axial Accelerometer and a Tri-Axial Magnetometer
Authors: Zhu X.N. , Ma M. , Cheng D.F. , Zhou Zh.J. 
Published: April 2018

Abstract
Tri-axial gyroscopes used to be the only instrument used to measure the angular increments of airplanes. However, because there were no reference devices, drift and accumulation errors affected the accuracy of the estimated attitude. In this paper, we propose a novel method for measuring angular increments based on a tri-axial accelerometer and a triaxial magnetometer. Then, we mathematically proved the feasibility of the proposed method. The results of our simulation and experimental tests indicated that the proposed method accurately indicates the attitude of an airplane.
Regime Shifts in Arctic Oscillation (AO) Variability 1951–2009
Authors: Marosz Michał 
Published: April 2018

Abstract
Arctic Oscillation (AO) reflects the dominating mode of SLP (or 1000hPa) variability in hemispheric scale and seems to bear an effect on the weather and, in longer time scales, on climate. The aim of the research was to identify the possible regime shifts in the multiannual course of AO index which in turn might be utilised in the identification and quantification of the regional/local meteorological field response. The data (AO monthly and daily index values) were acquired from the NOAA Climate Prediction Centre. The AO index is constructed by projecting monthly/daily anomalies of 1000hPa heights poleward of 20°N onto the loading pattern of the AO - 1st (leading) EOF of the 1000hPa height field for the same spatial domain. The temporal scope of analysis was 1951-2009. The analysis comprised the regime shift identification techniques that track the shifts in the mean values and in the magnitude of fluctuations as well as the usage of the classical linear trend identification in the annual and seasonal scale. The regime shift recognition was followed by the compositing method that allowed the analysis of spatial anomalies in SLP field in Euro-Atlantic region that reflect the response of the regional airflow to identified circulation regimes.
Microsatellite analysis of golden grey mullet Chelon auratus (Risso, 1810) in the Fereydoon-Kenar and Ramsar coasts (south Caspian Sea, Iran)
Authors: Behrouz Mohammad , Norouzi Mehrnoush , Samiei Mohammad Hadi , Heshmatzad Pouria 
Published: April 2018

Abstract
Genetic structure of golden grey mullet Chelon auratus investigated in the Fereydoon-Kenar and Ramsar coasts (south Caspian Sea, Iran), using 6 microsatellite markers designed for gray mullet (Mugil cephalus) and hand mullet (Liza haematocheila). A total of 60 samples of adult C. auratus were collected from these regions. All primer sets as polymorphic loci were used to analyze genetic variation. Analyses revealed that average of alleles (Na) per locus was 6.2 (range 3 to 9 alleles). All sampled regions contained private alleles. The average estimates of inbreeding coefficient (Fis) values of 6 microsatellites were positive. The average observed and expected heterozygosity was 0.394 and 0.743, respectively. Deviations from Hardy-Weinberg equilibrium were in all cases (P<0.001). F-statistics (Fst) and gene flow (Nm) estimates in allele frequencies were 0.078 and 2.9, respectively. Fst estimates in AMOVA indicated significant genetic differentiation among regions (P<0.01). Genetic distance was 0.679 indicating that the genetic difference among the studied populations is pronounced. The data generated in this study provides genetic variation and differentiation in populations of C. auratus in the southern Caspian Sea.
Agrobiodiversity Genetic Variability Utilization in Organic Food Production
Authors: Dimitrijević Miodrag , Petrović Sofija , Banjac Borislav , Barać Goran , Mladenov Velimir 
Published: April 2018

Summary
The food production at the global level is about to meet its border. Industrialization of agriculture correlates with an explosive enlargement of human population, during XX and at the beginning of XXI centuries. An ongoing process of environmental erosion has been speeding up during that period, not only in our physical surrounding, but also in biodiversity. A new direction in agricultural food production is in demand. Organic food production has been recognized as the way of providing safety and quality food, preserving the environment in the same time. In the other hand new land areas have to be explored for agricultural use, in order to enhance food quantity to meeting the increasing demand for food. These targets set new requirements in plant breeding. To fulfill these requirements the genetic variability harbored in genetic resources has to be preserved, examined and put to good use.
Effect of Indigenous Pseudomonas sp. and Bacillus sp. Strains on Yield and Main Chemical Growth Parameters of Radicchio
Authors: Stanojković-Sebić Aleksandra , Pivić Radmila , Dinić Zoran , Iličić Renata , Latković Dragana , Jošić Dragana 
Published: April 2018

Summary
Pseudomonas sp. and Bacillus sp. belong to plant growth promoting rhizobacteria which are able to colonize the plants roots and stimulate growth. In this study, the effect of two indigenous plant growth promoting rhizobacterial strains Pseudomonas sp. Q4 and Bacillus sp. Q10 and their mixture (mix Q4+Q10) on content of the main chemical growth parameters (nitrogen, phosphorus, potassium, calcium and magnesium) and the yield of dry biomass of radicchio (Cichorium spp. var. rossa di treviso) aerial parts and root, was investigated. The study was carried out with stagnosol type of soil in pot experiments under semi-controlled conditions in the Institute of Soil Science (Belgrade), in the period from July to October in 2013. Phosphorus was determined by spectrophotometer, potassium - by flame emission photometry and total nitrogen and carbon - using elemental CNS analyzer, while calcium and magnesium were determined by AAS. The data on yield of both aerial parts and root dry biomass of radicchio showed that its treatment with Q4 and Q10 strains, as well as with their mixture, caused noticeably increase in this parameter in relation to the control, whereby the strain Q4 was more effective for aerial parts, while mix Q4+Q10 - for roots. The obtained data on the studied chemical parameters of radicchio root and aerial parts were in total accordance with their yield. Concluding, studied strains have a potential in promoting the biomass yield and main chemical growth parameters of both aerial parts and root of radicchio.
Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats
Authors: Karimi-Sales Elham , Jeddi Sajad , Ghaffari-Nasab Arshad , Salimi Mina , Alipour Mohammad Reza 
Published: April 2018

Abstract
Objective. Trans-chalcone is a chalcone with hepatoprotective and anti-inflammatory effects. However, the mechanism of these positive effects, especially on miR-451 as an inflammatory regulator, is poorly understood. In this regard, this microRNA (miRNA) acts by inhibition of hepatic interleukin-8 (IL-8) production in the liver which is one of the main proinflammatory cytokines. Th is study for the first time examined the effect of trans-chalcone on miR-451/IL-8 pathway. Methods. In present study, 21 male rats were randomly divided into 3 groups (n=7 per each group): control which received solvent (NS), groups 2 (N2T) and 3 (N6T), which received transchalcone for 2 and 6 weeks, respectively. Hepatic level of miR-451 was measured by qRT-PCR. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as well as hepatic level of IL-8 protein were measured. Results. Trans-chalcone decreased hepatic level of IL-8 protein and serum level of ALT aft er 2 weeks of treatment without significant change in hepatic miR-451. Moreover, it increased hepatic level of miR-451 and reduced hepatic IL-8 as well as AST and ALT aft er 6 weeks. Conclusion. Based on the results of present study, miR-451/IL-8 pathway is a possible mechanism for hepatoprotective action of trans-chalcone in long-term.
Genetic and epigenetic differences of benign and malignant pheochromocytomas and paragangliomas (PPGLs)
Authors: Khatami Fatemeh , Mohammadamoli Mahsa , Tavangar Seyed Mohammad 
Published: April 2018

Abstract
Pheochromocytomas and paragangliomas (PPGLs) are tumors arising from the adrenal medulla and sympathetic/parasympathetic paraganglia, respectively. According to Th e Cancer Genome Atlas (TCGA), approximately 40% of PPGLs are due to germ line mutations in one of 16 susceptibility genes, and a further 30% are due to somatic alterations in at least seven main genes (VHL, EPAS1, CSDE1, MAX, HRAS, NF1, RET, and possibly KIF1B). Th e diagnosis of malignant PPGL was straight forward in most cases as it was defined as presence of PPGL in non-chromaffin tissues. Accordingly, there is an extreme need for new diagnostic marker(s) to identify tumors with malignant prospective. Th e aim of this study was to review all suggested genetic and epigenetic alterations that are remarkably different between benign and malignant PPGLs. It seems that more than two genetic mutation clusters in PPGLs and other genetic and methylation biomarkers could be targeted for malignancy discrimination in different studies.
Between Smallholder Traditions and “Ecological Modernisation” – Agricultural Transformation, Landscape Change and the Cap in Austria 1995–2015
Authors: Kurz Peter 
Published: April 2018

Abstract
The paper explores transformations in agriculture during the period 1995–2015 and shows their impact on rural landscapes in the case of Austria. When Austria joined the European Union in 1995, this meant a minor gash in agricultural politics, from broad support of smallholder agriculture to a programme of modernisation and rationalisation. Austrian politicians defined this shift as a process of “ecological modernisation” (Fischler et al. 1994), incorporating agri-environmental schemes as instruments and modifying existing programmes of direct payments. The survey forms the groundwork for a discussion on landscape effects of the CAP as an “ecological” modernisation programme and possible impact of the CAP-reform 2020.