bluuebunny/crossref_metadata_2025_split huggingface dataset:
Culture surfaces induce hypoxia-regulated genes in human mesenchymal stromal cells
Authors: Zhang Bo , Kasoju Naresh , Li Qiongfang , Soliman Erfan , Yang Aidong , Cui Zhanfeng , Ma Jinmin , Wang Hui , Ye Hua 
Published: March 2019

AbstractCulturing human Mesenchymal stromal cells (hMSCs)in vitroin hypoxic conditions resulted in reduced senescence, enhanced pluripotency and altered proliferation rate. It has been known thatin vitrohypoxia affects expression of cell surface proteins. However, the impact of culture surfaces on the hypoxia-regulated genes (HRG) have not yet been reported. This study utilized Next-Generation sequencing to analyse the changes in the gene expression levels of HRG for hMSCs cultured on different culture surfaces. The samples, which were cultured on four different synthesized surfaces (treatments) and tissue culture plate (control), resulted in a difference in growth rate. The sequencing results revealed that the transcription of a number of key genes involved in regulating hypoxic functions were significantly altered, includingHIF2A, a marker for potency, differentiation, and various cellular functions. Significant alternations in the expression levels of previously reported oxygen-sensitive surface proteins were detected in this study, some of which closely correlate with the expression levels ofHIF2A. Our analysis of the hMSCs transcriptome and HRG mapped out a list of genes encoding surface proteins which may directly regulate or be regulated byHIF2A. The findings from this study showed that culture surfaces have an impact on regulating the expression profile of HRG. Therefore, novel culture surfaces may be designed to selectively activateHIF2Aand other HRG and pathways underin vitronormoxia. The understanding of the crosstalk between the regulating genes of hypoxia and culture surfaces may be utilized to strengthen desired hypoxic functions.
A feature-based approach to predict hot spots in protein–DNA binding interfaces
Authors: Zhang Sijia , Zhao Le , Zheng Chun-Hou , Xia Junfeng 
Published: March 2019

Abstract
DNA-binding hot spot residues of proteins are dominant and fundamental interface residues that contribute most of the binding free energy of protein–DNA interfaces. As experimental methods for identifying hot spots are expensive and time consuming, computational approaches are urgently required in predicting hot spots on a large scale. In this work, we systematically assessed a wide variety of 114 features from a combination of the protein sequence, structure, network and solvent accessible information and their combinations along with various feature selection strategies for hot spot prediction. We then trained and compared four commonly used machine learning models, namely, support vector machine (SVM), random forest, Naïve Bayes and k-nearest neighbor, for the identification of hot spots using 10-fold cross-validation and the independent test set. Our results show that (1) features based on the solvent accessible surface area have significant effect on hot spot prediction; (2) different but complementary features generally enhance the prediction performance; and (3) SVM outperforms other machine learning methods on both training and independent test sets. In an effort to improve predictive performance, we developed a feature-based method, namely, PrPDH (Prediction of Protein–DNA binding Hot spots), for the prediction of hot spots in protein–DNA binding interfaces using SVM based on the selected 10 optimal features. Comparative results on benchmark data sets indicate that our predictor is able to achieve generally better performance in predicting hot spots compared to the state-of-the-art predictors. A user-friendly web server for PrPDH is well established and is freely available at http://bioinfo.ahu.edu.cn:8080/PrPDH.
Impact of a tailored surgical approach on autograft root dimensions in patients undergoing the Ross procedure for aortic regurgitation†
Authors: Bouhout Ismail , Ghoneim Aly , Tousch Michael , Stevens Louis Mathieu , Semplonius Trevor , Tarabzoni Mohammed , Poirier Nancy , Cartier Raymond , Demers Philippe , Guo Linrui , Chu Michael W A , El-Hamamsy Ismail 
Published: March 2019

Abstract

OBJECTIVES
The Ross procedure in patients with aortic regurgitation (AR) has been associated with increased autograft dilatation and late reintervention. The aim of this study was to evaluate the impact of a tailored approach aimed at mitigating that risk on early changes in autograft root dimensions following the Ross procedure in patients with AR.

METHODS
From 2011 to 2018, 241 consecutive patients underwent a Ross procedure with >1 year of follow-up [46 (7) years]. Aortic root dimensions were prospectively measured on serial echocardiograms. Patients with aortic stenosis group (n = 171; 71%) were compared to those with AR or mixed aortic disease (AR group) (n = 70; 29%). Mean length of follow-up was 29 ± 11 months (100% complete). Changes in aortic dimensions were analysed using mixed-effect models.

RESULTS
At 4 years, mean indexed diameters of the annulus, sinuses of Valsalva and the sinotubular junction in the AR group were 12.3 (0.2) mm/m2, 20.0 (0.4) mm/m2 and 16.3 (0.9) mm/m2, respectively, vs 11.9 (0.2), 18.4 (0.3) and 15.5 (0.5) in the aortic stenosis group. Overall, there were no significant differences in the rates of autograft annulus, sinuses of Valsalva and sinotubular junction dimension changes between the aortic stenosis and AR groups up to 4 years after surgery (P = 0.55, P = 0.12, P = 0.59 and P = 0.48, respectively).

CONCLUSIONS
Use of a tailored surgical approach, combined with a strict blood pressure control, appears to mitigate clinically significant early dilatation of the autograft root following a Ross procedure in patients with AR. Further follow-up is needed to determine if this will translate into a lower incidence of long-term reintervention.
Pregnancy outcomes in women with cardiovascular disease: evolving trends over 10 years in the ESC Registry Of Pregnancy And Cardiac disease (ROPAC)
Authors: Roos-Hesselink Jolien , Baris Lucia , Johnson Mark , De Backer Julie , Otto Catherine , Marelli Ariane , Jondeau Guillaume , Budts Werner , Grewal Jasmine , Sliwa Karen , Parsonage William , Maggioni Aldo P , van Hagen Iris , Vahanian Alec , Tavazzi Luigi , Elkayam Uri , Boersma Eric , Hall Roger 
Published: March 2019

Abstract

Aims
Reducing maternal mortality is a World Health Organization (WHO) global health goal. Although maternal deaths due to haemorrhage and infection are declining, those related to heart disease are increasing and are now the most important cause in western countries. The aim is to define contemporary diagnosis-specific outcomes in pregnant women with heart disease.

Methods and results
From 2007 to 2018, pregnant women with heart disease were prospectively enrolled in the Registry Of Pregnancy And Cardiac disease (ROPAC). Primary outcome was maternal mortality or heart failure, secondary outcomes were other cardiac, obstetric, and foetal complications. We enrolled 5739 pregnancies; the mean age was 29.5. Prevalent diagnoses were congenital (57%) and valvular heart disease (29%). Mortality (overall 0.6%) was highest in the pulmonary arterial hypertension (PAH) group (9%). Heart failure occurred in 11%, arrhythmias in 2%. Delivery was by Caesarean section in 44%. Obstetric and foetal complications occurred in 17% and 21%, respectively. The number of high-risk pregnancies (mWHO Class IV) increased from 0.7% in 2007–2010 to 10.9% in 2015–2018. Determinants for maternal complications were pre-pregnancy heart failure or New York Heart Association &gt;II, systemic ejection fraction &lt;40%, mWHO Class 4, and anticoagulants use. After an increase from 2007 to 2009, complication rates fell from 13.2% in 2010 to 9.3% in 2017.

Conclusion
Rates of maternal mortality or heart failure were high in women with heart disease. However, from 2010, these rates declined despite the inclusion of more high-risk pregnancies. Highest complication rates occurred in women with PAH.
Habitat suitability modelling revealing environmental-driven abundance variability and geographical distribution shift of winter–spring cohort of neon flying squid Ommastrephes bartramii in the northwest Pacific Ocean
Authors: Yu Wei , Chen Xinjun , Zhang Yang , Yi Qian 
Published: March 2019

Abstract
To identify climate-related habitat changes and variations in abundance and distribution of Ommastrephes bartramii in the northwest Pacific Ocean, an habitat suitability index (HSI) model was developed in this study including sea surface temperature (SST), photosynthetically active radiation (PAR), and sea surface height anomaly (SSHA). The catch-per-unit-effort (CPUE) of O. bartramii gradually decreased between 2006 and 2015, and the latitudinal gravity centres (LATG) of the fishing effort shifted southward. Correlation analyses suggested that CPUE was positively related to SST, PAR, and the areas of suitable and optimal habitat, but negatively correlated with SSHA and the percentages of poor habitat. A significantly positive correlation was found between the LATG and the average latitude of the most preferred SST, PAR, and the average latitude of the area with an HSI between 0.9 and 1.0. From 2006 to 2015, the annual declined CPUE was highly consistent with the increase in areas of poor habitat and the reduction in areas of suitable and optimal habitats. The south-approaching LATG coincided with the southward migration pattern of the latitude of the HSI area in the range of 0.9–1.0. Moreover, compared to the El Niño events, the La Niña events, and normal climate condition yielded enlarged suitable habitat areas for O. bartramii, and the LATG moved further north.
Defending the “Acquisition-Use Presumption” in Assessing the Likelihood of Nuclear Terrorism
Authors: Bell Mark S 
Published: March 2019

AbstractIn an important article, McIntosh and Storey (2018) challenge the “acquisition-use presumption” that a terrorist organization with a nuclear weapon would inevitably seek to detonate it in an attack. They argue that a terrorist organization with nuclear weapons has more attractive options than conducting a direct nuclear attack, that organizational politics mean that a terrorist organization with a nuclear weapon would be unlikely to seek to detonate it, and that a nuclear attack would escalate the threats the terrorist organization faced. I argue that these arguments are ultimately unpersuasive and that the acquisition-use presumption remains a valid basis for theorizing about the likelihood of nuclear terrorism.
Thermal Tolerance Plasticity in Chagas Disease Vectors Rhodnius prolixus (Hemiptera: Reduviidae) and Triatoma infestans
Authors: Belliard Silvina A , De la Vega Gerardo J , Schilman Pablo E 
Published: March 2019

AbstractTemperature is recognized as the most influential abiotic factor on the distribution and dispersion of most insect species including Rhodnius prolixus (Stål, 1859) and Triatoma infestans (Klug, 1834), the two most important Chagas disease vectors. Although, these species thermotolerance range is well known their plasticity has never been addressed in these or any other triatomines. Herein, we investigate the effects of acclimation on thermotolerance range and resistance to stressful low temperatures by assessing thermal critical limits and ‘chill-coma recovery time’ (CCRT), respectively. We found positive effects of acclimation on thermotolerance range, especially on the thermal critical minimum of both species. In contrast, CCRT did not respond to acclimation in either. Our results reveal the plasticity of these Triatomines thermal tolerance in response to a wide range of acclimation temperatures. This presumably represents a physiological adaptation to daily or seasonal temperature variation with concomitant improvement in dispersion potential.
A Realis Subjunctive in German
Authors: Csipak Eva 
Published: March 2019

AbstractThe German Konjunktiv II is known for its reportative and irrealis uses. This paper argues for a third, realis, use which is independent of the other two uses. Thus by uttering ‘Da wäre Saft im Kühlschrank there is.[realis subjunctive] juice in the fridge’ a speaker can signal that not only is she certain that there is juice in the fridge, but also that she is offering the juice to an interlocutor. I show that the meaning contribution of the realis subjunctive is strictly not-at-issue, and I develop a multi-dimensional formal semantics that captures both the distribution of the realis subjunctive, and its interaction with operators such as negation and tense. The conditions of use of the construction are modelled in terms of decision theory.
Media Framing Dynamics of the ‘European Refugee Crisis’: A Comparative Topic Modelling Approach
Authors: Heidenreich Tobias , Lind Fabienne , Eberl Jakob-Moritz , Boomgaarden Hajo G 
Published: March 2019

Abstract
The complexity and duration of the so-called ‘European refugee crisis’ created a climate of uncertainty, which left ample room for mass media to shape citizens’ understanding of what the arrival of these refugees meant for their respective country. This study analyses the national media discourses in Hungary, Germany, Sweden, the United Kingdom and Spain for this time period. Applying Latent Dirichlet Allocation topic modelling in five languages and based on N = 130,042 articles from 24 news outlets, we reveal country-specific media frames to track the overall course of the refugee debate and to uncover dynamics and shifts in discourses. While results show similarities across countries, due to media coverage responding to real-world developments, there are differences in media framing as well. Possible sources of these differences such as countries’ geographic location or status as receiving country are discussed.
Imprints of r-process heating on fall-back accretion: distinguishing black hole–neutron star from double neutron star mergers
Authors: Desai D , Metzger B D , Foucart F 
Published: March 2019

ABSTRACT
Mergers of compact binaries containing two neutron stars (NS–NS), or a neutron star and a stellar mass black hole (NS–BH), are likely progenitors of short-duration gamma-ray bursts (SGRBs). A fraction ${\gtrsim } 20{{\ \rm per\ cent}}$ of SGRBs is followed by temporally extended (≳minute-long), variable X-ray emission, attributed to ongoing activity of the central engine. One source of late-time engine activity is fall-back accretion of bound tidal ejecta; however, observed extended emission light curves do not track the naively anticipated, uninterrupted t−5/3 power-law decay, instead showing a lull or gap in emission typically lasting tens of seconds after the burst. Here, we re-examine the impact of heating due to rapid neutron capture (r-process) nucleosynthesis on the rate of the fall-back accretion, using ejecta properties extracted from numerical relativity simulations of NS–BH mergers. Heating by the r-process has its greatest impact on marginally bound matter, hence its relevance to late-time fall-back. Depending on the electron fraction of the ejecta and the mass of the remnant black hole, r-process heating can imprint a range of fall-back behaviour, ranging from temporal gaps of up to tens of seconds to complete late-time cut-off in the accretion rate. This behaviour is robust to realistic variations in the nuclear heating experienced by different parts of the ejecta. Central black holes with masses ${\lesssim } 3\, \mathrm{M}_{\odot }$ typically experience absolute cut-offs in the fall-back rate, while more massive ${\gtrsim } 6\!-\!8\, \mathrm{M}_{\odot }$ black holes instead show temporal gaps. We thus propose that SGRBs showing extended X-ray emission arise from NS–BH, rather than NS–NS, mergers. Our model implies an NS–BH merger detection rate by LIGO that, in steady state, is comparable to or greater than that of NS–NS mergers.
On a conjecture of additive complements
Authors: Fang Jin-Hui , Chen Yong-Gao 
Published: March 2019

AbstractFor two infinite sequences A and B of non-negative integers, if every sufficiently large integer can be expressed as the sum of two elements taken from A and B, then we call such A, B additive complements. Let A(x) (resp. B(x)) be the counting functions of A (resp. B). Motivated by a result of Sárközy and Szemerédi, the authors proved that if limsupA(x)B(x)/x<3−3 or limsupA(x)B(x)/x>2, then A(x)B(x)−x→+∞ as x→+∞. Afterwards we also posed a natural conjecture that: for additive complements A, B, there exists a set T of non-negative integers with density one such that A(x)B(x)−x→+∞asx∈Tandx→+∞. In this paper, we confirm this conjecture. Furthermore, we obtain a stronger evaluation for the special additive complements.
Integrin binding dynamics modulate ligand-specific mechanosensing in mammary gland fibroblasts
Authors: Lerche Martina , Elosegui-Artola Alberto , Kechagia Jenny Z. , Guzmán Camilo , Georgiadou Maria , Gullberg Donald , Roca-Cusachs Pere , Peuhu Emilia , Ivaska Johanna 
Published: March 2019

SummaryThe link between the modulation of integrin activity and cellular mechanosensing of tissue rigidity, especially on different extracellular matrix ligands, remains poorly understood. Here, we find that primary mouse mammary gland stromal fibroblasts (MSFs) are able to spread efficiently on soft collagen-coated substrates, resembling the soft mammary gland tissue. In addition, MSFs generate high forces and display nuclear YAP at a low matrix stiffness, supported by mature focal adhesions, prominent actin stress fibers, and myosin phosphorylation.We describe that loss of the cytosolic integrin inhibitor, SHARPIN, impedes MSF spreading specifically on soft type I collagen but not on fibronectin. Through quantitative experiments and computational modelling, we find that SHARPIN-deficient MSFs display faster force-induced unbinding of adhesions from collagen-coated beads. Faster unbinding, in turn, impairs force transmission in these cells, particularly, at the stiffness optimum observed for wild-type cells, and increases actin retrograde flow. Mechanistically, we link the impaired mechanotransduction of SHARPIN-deficient cells on collagen to reduced levels of the collagen-binding integrin α11β1. Our results unveil a collagen-specific mechanosensing mechanism and suggest a key function for integrin activity regulation and integrin α11β1 in MSF mechanotransduction.
Identification of differentially expressed genes in hepatocellular carcinoma by integrated bioinformatic analysis
Authors: Yan Guangxin , Liu Zhaoyu 
Published: March 2019

AbstractHepatocellular carcinoma is one of the most common tumors in the world and has a high mortality rate. This study elucidates the mechanism of hepatocellular carcinoma- (HCC) related development. The HCC gene expression profile (GSE54238, GSE84004) was downloaded from Gene Expression Omnibus for comprehensive analysis. A total of 359 genes were identified, of which 195 were upregulated and 164 were downregulated. Analysis of the condensed results showed that “extracellular allotrope” is a substantially enriched term. “Cell cycle”, “metabolic pathway” and “DNA replication” are three significantly enriched Kyoto Encyclopedia of Genes and Genomespathways. Subsequently, a protein-protein interaction network was constructed. The most important module in the protein-protein interaction network was selected for path enrichment analysis. The results showed thatCCNA2, PLK1, CDC20, UBE2CandAURKAwere identified as central genes, and the expression of these five hub genes in liver cancer was significantly increased in The Cancer Genome Atlas. Univariate regression analysis was also performed to show that the overall survival and disease-free survival of patients in the high expression group were longer than in the expression group. In addition, genes in important modules are mainly involved in “cell cycle”, “DNA replication” and “oocyte meiosis” signaling pathways. Finally, through upstream miRNA analysis, mir-300 and mir-381-3p were found to coregulateCCNA2,AURKAandUBE2C. These results provide a set of targets that can help researchers to further elucidate the underlying mechanism of liver cancer.
Loneliness, regulatory focus, inter-personal competence, and online game addiction
Authors: Lee Ji-yeon , Ko Dong Woo , Lee Hyemin 
Published: March 2019

Purpose
The purpose of this paper is to investigate the predictors of game addiction based on loneliness, motivation and inter-personal competence using the samples of college students recruited from South Korea (n=251).

Design/methodology/approach
The authors examined the underlying mechanism of game addiction by testing a moderated mediation model, in which inter-personal competence moderated the mediation model of loneliness, regulatory focus and online game addiction. First, the authors clarified the relationship among loneliness, motivation and inter-personal competence, to understand the influences of loneliness on other variables in this study (mediation test). Second, the authors examined the underlying mechanism of game addiction by testing a moderated mediation model, in which inter-personal competence moderated the mediation model of loneliness, regulatory focus and online game addiction (moderated mediation).

Findings
Regulatory focus mediated the effect of loneliness on online game addiction. Moderated mediation analyses using PROCESS confirmed that inter-personal competence significantly buffered the indirect effect of loneliness (through regulatory focus) on online game addiction. The findings indicated that inter-personal competence accounted for significant differences in the mediation models.

Originality/value
This study bridges the gap in the online game addiction literature by explaining how loneliness is associated with online game addiction.
Impact of climate change on yield and water requirement of rainfed crops in the Setif region
Authors: Bouregaa Tarek 
Published: March 2019

Purpose
The purpose of this paper is to show the impact of climate change on yield and water requirement of three rainfed crops in Setif region.

Design/methodology/approach
This study investigates likely changes in annual temperature and precipitation over Setif high plains region (North East of Algeria) among three future periods: 2025, 2050 and 2075. The projections are based on the SRES A2 and B2 scenarios. MAGICC-SCENGEN 5.3 v.2 was used as a tool for downscaling the four selected general circulation models (GCMs) output data. The expected impact of climate change on yield and water requirement of winter wheat, barley and olive was evaluated using the CROPWAT model.

Findings
The projection of the four GCMs showed that average temperature will increase by 0.73 to 3.42°C, and the precipitation will decrease by 1 to 52.7 percent, across the three future periods under the two SRES scenarios. Winter wheat and olive yields are expected to decrease under the three types of soils (heavy, medium and light). However, barley yield is expected to reduce under light soil only. Crop water requirements and irrigation water requirements are expected to increase under the two scenarios and across the three future periods.

Originality/value
This research is one of the first to study the impact of future climate change on water requirement and yield of rainfed crops over Setif region.