bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of antitumor effect of oxygen nanobubble water on breast cancer‐bearing BALB/c mice
Authors: Mahjour Azita , Khazaei Majid , Nourmohammadi Esmail , Khoshdel‐Sarkarizi Hoda , Ebrahimzadeh‐Bideskan Alireza , Rahimi Hamid Reza , Safipour Afshar Akbar 
Published: May 2019

AbstractHypoxia is a condition of low oxygen level which poses a common feature of most cancers. In the current study, we investigated effect of water containing oxygen nanobubble (ONB) on tumor growth in breast cancer 4T1‐bearing mice during 14‐day treatment period. Tumor‐bearing mice were randomly divided into three groups (six mice per group), including the ONB group drinking water containing ONB, the air nanobubble (ANB) group drinking water containing ANB, and control group drinking normal water. Tumor weight and size were measured in 2‐day interval during 14‐day treatment. mRNA expression of p53, vascular endothelial growth factor (VEGF), hypoxia‐inducible factor (HIF), and cyclin D/Cdk2 genes were measured in the treated and control mice. After 8, 12, and 14 days of treatment, tumor size in ONB group was significantly decreased by 40.5%, 32.8%, and 28%, respectively, when compared with the control group. In addition, ANB group showed a significant reduction in tumor burden as well. The messenger RNA (mRNA) level of p53 in tumor cells of ONB and ANB group was found to be 36‐fold (P = 0.0001) and 33‐fold (P = 0.0001) higher than that in the control group, respectively. There was a ninefold increase in mRNA expression of VEGF gene in tumor cells of ANB mice than that in control mice; however, there was no significant changes in ONB group. Expression of HIF gene was significantly lower in tumor cells of ONB and ANB group than in the control group. It is concluded that drinking ONB water has potential to inhibit tumor growth, however more preclinical and proof‐of‐concept studies are needed to confirm its safety and therapeutic effect.
GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562
Authors: Riccio Patrizia , Sessa Raffaele , de Nicola Sergio , Petruzziello Fara , Trombetti Silvia , Menna Giuseppe , Pepe Giampiero , Maddalena Pasquale , Izzo Paola , Grosso Michela 
Published: May 2019

AbstractMaintenance of a balanced expression of the two isoforms of the transcription factor GATA‐1, the full‐length protein (GATA‐1FL) and a shorter isoform (GATA‐1
S), contributes to control hematopoiesis, whereas their dysregulation can alter the differentiation/proliferation potential of hematopoietic precursors thereby eventually leading to a variety of hematopoietic disorders. Although it is well established that these isoforms play opposite roles in these remarkable processes, most of the molecular pathways involved remain unknown.Here, we demonstrate that GATA‐1FL and GATA‐1S are able to differently influence intracellular redox states and reactive oxygen species (ROS) compartmentation in the erythroleukemic K562 cell line, thus shedding novel mechanistic insights into the processes of cell proliferation and apoptosis resistance in myeloid precursors. Furthermore, given the role played by ROS signaling as a strategy to escape apoptosis and evade cell‐mediated immunity in myeloid cells, this study highlights a mechanism through which aberrant expression of GATA‐1 isoforms could play a role in the leukemogenic process.
CD markers variations in chronic lymphocytic leukemia: New insights into prognosis
Authors: Vosoughi Tina , Bagheri Marziye , Hosseinzadeh Mehran , Ehsanpour Ali , Davari Nader , Saki Najmaldin 
Published: May 2019

AbstractChronic lymphocytic leukemia (CLL) is one of the most commonly occurring adult leukemias that is associated with clonal accumulation of mature apoptosis‐resistant B‐cells in bone marrow, peripheral blood, and specific tissues. Different pathogenesis factors can contribute to the aggression of the clinical course in this disease. Cytogenetic abnormalities and surface biomarkers of neoplastic CLL cells can be effective in the outcome of CLL, and the examination of changing CD markers expressions in the progression of CLL can be related to the prognosis of this disease. Changing expression levels of CD markers on lymphocytes and other cells in CLL patients can play a role in the aggressive clinical outcomes such as organomegaly, immunodeficiency, and advanced disease stages through their interaction with CLL microenvironment. Given the involvement of CD markers in the pathogenesis of CLL, it can be stated that recognizing the expression changes of CD markers in the cells involved in CLL can be a proper approach to evaluate prognosis among these patients.
Shelf‐life study of ready‐to‐cook slices of globe artichoke ‘Spinoso sardo’: effects of anti‐browning solutions and edible coating enriched withFoeniculum vulgareessential oil
Authors: Rizzo Valeria , Lombardo Sara , Pandino Gaetano , Barbagallo Riccardo N , Mazzaglia Agata , Restuccia Cristina , Mauromicale Giovanni , Muratore Giuseppe 
Published: May 2019

AbstractBackgroundThe globe artichoke [Cynara cardunculusvar.scolymus(L.) Fiori] is a natural source of minerals, fiber, inulin, and polyphenols. A ready‐to‐cook (RTC) version of this product could improve its commercialization and consumption. In this study, the effects of (i) anti‐browning treatments (citric acid 0.5% + ascorbic acid 2%, or cysteine 0.5%, w/v) and (ii) dipping in locust bean gum (LBG) edible coating with or withoutFoeniculum vulgareessential oil (EO) were evaluated on RTC globe artichoke slices cv. ‘Spinoso sardo’ during storage (11 days at 4 °C). Fresh weight loss, color, texture, polyphenol oxidase (PPO), microbiological and chemical parameters, antioxidant capacity, and sensory descriptors were determined.ResultsThe results showed that, despite the antioxidant treatment used, when EO was added to LBG, all microbiological groups underwent an average reduction of 0.50 log CFU g−1. Cysteine preserved the antioxidant capacity, color, and texture of samples better than citric acid and ascorbic acid, and inhibited PPO; the addition of EO to LBG enhanced these effects. At the end of the storage period, the overall highest sensory attribute score was attained by the coated globe artichoke samples with EO.ConclusionThe marked efficacy of the LBG edible coating with addedF. vulgareEO in maintaining microbiological, physical, chemical, and sensory qualities makes it a promising processing tool for the preservation of ready‐to‐cook Spinoso sardo globe artichoke slices during storage for 11 days at 4 °C. © 2019 Society of Chemical Industry
Screening the anti‐allergic components in Saposhnikoviae Radix using high‐expression Mas‐related G protein‐coupled receptor X2 cell membrane chromatography online coupled with liquid chromatography and mass spectrometry
Authors: Jia Qianqian , Sun Wei , Zhang Liyang , Fu Jia , Lv Yanni , Lin Yuanyuan , Han Shengli 
Published: May 2019

AbstractSaposhnikoviae Radix, the dried root of Saposhnikoviae divaricata, is commonly used in the traditional Chinese anti‐allergic preparations, like Bofutsusho‐san and Yupingfeng granules. A high‐expression Mas‐related G protein‐coupled receptor X2 cell membrane chromatography coupled online with high‐performance liquid chromatography combined with an ion trap time‐of‐flight multistage mass spectrometry system was established and used for screening and identifying the anti‐allergic components in Saposhnikoviae Radix. The system was validated for excellent specificity and suitability using the appropriate standards. Two retained fractions were obtained on the cell membrane chromatography column, and three main components were identified as prim‐O‐glucosylcimifugin, cimifugin, and 4′‐O‐β‐d‐glucosyl‐5‐O‐methylvisamminol. Next, the molecular docking study was conducted, which confirmed that these three components could effectively bind to MRGPRX2 through hydrogen bonds with its amino acid residues. Finally, histamine release assay was performed to investigate the bioactivities of prim‐O‐glucosylcimifugin, cimifugin, and 4′‐O‐β‐d‐glucosyl‐5‐O‐methylvisamminol. Results showed that these three components could exert anti‐allergic effects by inhibiting the histamine release in a dose‐dependent manner (from 10 to 100 µM). In conclusion, the high‐expression Mas‐related G protein‐coupled receptor X2 cell membrane chromatography is an effective tool for discovering the anti‐allergic components in Saposhnikoviae Radix.
Understanding memorable tourism experiences as the determinants of tourists' behaviour
Authors: Sharma Pramod , Nayak Jogendra Kumar 
Published: May 2019

AbstractThis study empirically investigated a theoretical model predicting the direct and indirect effects of memorable tourism experiences (MTEs) on behavioural intentions via destination image and tourists' satisfaction in yoga tourism. This research was conducted using data collected from 502 tourists visiting Rishikesh in India. Using confirmatory factor analysis and SPSS macro (PROCESS), findings confirmed that MTEs influenced behavioural intentions both directly and indirectly via destination image and tourists' satisfaction. MTEs were found as the strongest influencer of behavioural intentions in yoga tourism. This study opposes those researchers and practitioners who argued tourists' satisfaction alone as the most significant influencer of destination performance.
Constraints to domestic ocean cruise participation among higher income Chinese adults
Authors: Tang Chuanzhong , Weaver David , Shi Fangfang , Huang Ming‐Feng , Liu Yang 
Published: May 2019

AbstractThe study identifies barriers to participation in cruises from domestic homeports among higher income Chinese adults. Results from 775 respondents extend leisure constraints theory by revealing that “structural” constraints (lack of knowledge, time, and money) most influence intentions not to cruise even for these wealthier consumers, whereas “not‐an‐option” constraints (disinterest due to previous cruising experience, intentions to cruise from a foreign homeport) are least influential. “Personal” factors (lack of companions and fears about safety, boredom, and seasickness) are moderately important. Six identified clusters include a large normative “moderate‐constraint” cluster, small “low‐potential” and “high‐potential” segments, and several specialized segments.
Autologous hematopoietic stem cell transplantation as first‐line consolidation therapy can improve the prognosis of diffuse large B‐cell lymphoma with high expression of MYC protein
Authors: Hu Kai , Gao Jin‐Jie , Li Qi‐Hui , Tian Lei , Wan Wei , Zhao Wei , Wang Ji‐Jun , Fu Lin 
Published: May 2019

AbstractMyc‐positive diffuse large B‐cell lymphoma has lower curative efficacy and long‐term survival than its negative counterpart, even when treated with R‐CHOP regimen. The present study aims to determine whether the use of autologous hematopoietic stem cell transplantation as a consolidation therapy can improve the curative efficacy in this type of patients after achieving the best effect of chemotherapy for the first time. The data of 50 patients with Myc‐positive diffuse large B‐cell lymphoma were retrospectively analyzed. Autologous transplantation was performed for 23 patients, while transplantation was not performed for 27 patients. The clinicopathological features and survival conditions were compared. The 1‐year and 3‐year progression‐free survival (PFS) rates were 66.7% ± 0.9% and 57.7% ± 1.0%, respectively, in the non transplantation group, and 100% and 82.1% ± 0.1%, respectively, in the transplantation group (P = .021). The 1‐year overall survival (OS) rate for these two groups was 88.7% ± 0.6% vs 100%, respectively, while the 3‐year OS rates for these two groups was 78.6% ± 0.1% vs 91.3% ± 0.1%, respectively (P = .176). Hematopoietic stem cell transplantation performed after chemotherapy is a risk factor for OS. Autologous hematopoietic stem cell transplantation as a consolidation therapy in the early stage can improve the prognosis of patients with Myc‐positive diffuse large B‐cell lymphoma.
3D and Porous RGDC‐Functionalized Polyester‐Based Scaffolds as a Niche to Induce Osteogenic Differentiation of Human Bone Marrow Stem Cells
Authors: Yassin Mohammed A. , Fuoco Tiziana , Mohamed‐Ahmed Samih , Mustafa Kamal , Finne‐Wistrand Anna 
Published: May 2019

AbstractPolyester‐based scaffolds covalently functionalized with arginine‐glycine‐aspartic acid‐cysteine (RGDC) peptide sequences support the proliferation and osteogenic differentiation of stem cells. The aim is to create an optimized 3D niche to sustain human bone marrow stem cell (hBMSC) viability and osteogenic commitment, without reliance on differentiation media. Scaffolds consisting of poly(lactide‐co‐trimethylene carbonate), poly(LA‐co‐TMC), and functionalized poly(lactide) copolymers with pendant thiol groups are prepared by salt‐leaching technique. The availability of functional groups on scaffold surfaces allows for an easy and straightforward method to covalently attach RGDC peptide motifs without affecting the polymerization degree. The strategy enables the chemical binding of bioactive motifs on the surfaces of 3D scaffolds and avoids conventional methods that require harsh conditions. Gene and protein levels and mineral deposition indicate the osteogenic commitment of hBMSC cultured on the RGDC functionalized surfaces. The osteogenic commitment of hBMSC is enhanced on functionalized surfaces compared with nonfunctionalized surfaces and without supplementing media with osteogenic factors. Poly(LA‐co‐TMC) scaffolds have potential as scaffolds for osteoblast culture and bone grafts. Furthermore, these results contribute to the development of biomimetic materials and allow a deeper comprehension of the importance of RGD peptides on stem cell transition toward osteoblastic lineage.
Hsp104 facilitates the endoplasmic‐reticulum–associated degradation of disease‐associated and aggregation‐prone substrates
Authors: Doonan Lynley M. , Guerriero Christopher J. , Preston G. Michael , Buck Teresa M. , Khazanov Netaly , Fisher Edward A. , Senderowitz Hanoch , Brodsky Jeffrey L. 
Published: May 2019

AbstractMisfolded proteins in the endoplasmic reticulum (ER) are selected for ER‐associated degradation (ERAD). More than 60 disease‐associated proteins are substrates for the ERAD pathway due to the presence of missense or nonsense mutations. In yeast, the Hsp104 molecular chaperone disaggregates detergent‐insoluble ERAD substrates, but the spectrum of disease‐associated ERAD substrates that may be aggregation prone is unknown. To determine if Hsp104 recognizes aggregation‐prone ERAD substrates associated with human diseases, we developed yeast expression systems for a hydrophobic lipid‐binding protein, apolipoprotein B (ApoB), along with a chimeric protein harboring a nucleotide‐binding domain from the cystic fibrosis transmembrane conductance regulator (CFTR) into which disease‐causing mutations were introduced. We discovered that Hsp104 facilitates the degradation of ER‐associated ApoB as well as a truncated CFTR chimera in which a premature stop codon corresponds to a disease‐causing mutation. Chimeras containing a wild‐type version of the CFTR domain or a different mutation were stable and thus Hsp104 independent. We also discovered that the detergent solubility of the unstable chimera was lower than the stable chimeras, and Hsp104 helped retrotranslocate the unstable chimera from the ER, consistent with disaggregase activity. To determine why the truncated chimera was unstable, we next performed molecular dynamics simulations and noted significant unraveling of the CFTR nucleotide‐binding domain. Because human cells lack Hsp104, these data indicate that an alternate disaggregase or mechanism facilitates the removal of aggregation‐prone, disease‐causing ERAD substrates in their native environments.
Energy system transition and macroeconomic impacts of a European decarbonization action towards a below 2 °C climate stabilization
Authors: Vrontisi Zoi , Fragkiadakis Kostas , Kannavou Maria , Capros Pantelis 
Published: May 2019

Abstract
The European Union has recently established the “Clean Energy for all Europeans” climate policy framework, aiming at the achievement of the European Nationally Determined Contribution (NDC) submitted to the Paris Agreement. The EU28 NDC includes a commitment for emission reductions in 2030 but also refers to an economy-wide effort towards 2050 so as to contribute effectively to the long-term mitigation of climate change. We discuss the respective EU28 emission pathways in the context of a well below 2 °C global climate stabilization target and estimate the macroeconomic impacts for the EU28 economy by considering alternative levels of climate action for major non-EU emitters. We employ two models, the technology-rich energy system model PRIMES, and the global large-scale hybrid computable general equilibrium model GEM-E3. The two models are soft linked so as to ensure a consistent and robust framework of analysis. We find that emission reductions in the energy supply sector are dominant up to 2030 while transport takes the lead in 2050. Transport and non-CO2 emissions are the main remaining emitting sources in 2050. We present the key decarbonization pillars and confirm that the impacts on the EU28 economy largely depend on the level of mitigation action adopted by the rest of the world and by the relative carbon intensity across regions. Due to asymmetric ambition of climate policies, a global implementation of NDCs results in economic losses for the EU28 when compared with a “pre-Paris” policy reference scenario, despite positive effects on energy-intensive and clean technology exports. On the contrary, we find that the region registers economic gains in the case of coordinated 2 °C global climate action.
Crushed Gold Shell Nanoparticles Labeled with Radioactive Iodine as a Theranostic Nanoplatform for Macrophage-Mediated Photothermal Therapy
Authors: Lee Sang Bong , Lee Jae-Eon , Cho Sung Jin , Chin Jungwook , Kim Sang Kyoon , Lee In-Kyu , Lee Sang-Woo , Lee Jaetae , Jeon Yong Hyun 
Published: May 2019

Abstract
Plasmonic nanostructure-mediated photothermal therapy (PTT) has proven to be a promising approach for cancer treatment, and new approaches for its effective delivery to tumor lesions are currently being developed. This study aimed to assess macrophage-mediated delivery of PTT using radioiodine-124-labeled gold nanoparticles with crushed gold shells (124I-Au@AuCBs) as a theranostic nanoplatform. 124I-Au@AuCBs exhibited effective photothermal conversion effects both in vitro and in vivo and were efficiently taken up by macrophages without cytotoxicity. After the administration of 124I-Au@AuCB-labeled macrophages to colon tumors, intensive signals were observed at tumor lesions, and subsequent in vivo PTT with laser irradiation yielded potent antitumor effects. The results indicate the considerable potential of 124I-Au@AuCBs as novel theranostic nanomaterials and the prominent advantages of macrophage-mediated cellular therapies in treating cancer and other diseases.
Intra-arterial Thrombolysis for Retinal Artery Occlusion: The Calgary Experience
Authors: Pettersen J.A. , Hill M.D. , Dechuck A.M. , Morrish W. , Hudon M.E. , Hu W. , Wong J. , Barber P.A. , Buchan A.M. 
Published: May 2019

ABSTRACT:Introduction:Retinal artery occlusion represents a medical emergency with poor prognosis for visual recovery. Spontaneous improvement is estimated to occur in less than 15% of central retinal artery occlusion (CRAO) cases and conventional treatments have provided only limited benefit. Intra-arterial thrombolysis has been reported as a potentially efficacious and safe treatment.Methods:We performed a retrospective chart review of all retinal artery occlusion cases treated with intra-arterial recombinant tissue-type plasminogen activator (rtPA) from January 1998 to May 2004. Patients received Goldmann perimetry visual field testing at a variable interval following the procedure (2 days-2.5 years). Visual acuity (VA) was re-assessed in May 2004.Results:Eight cases (59-77 years) were treated for CRAO, 6-18 hours post-onset with intra-arterial rtPA (10-20 mg over 15-60 minutes); one case of branch occlusion (BRAO) was treated with 30 mg rtPA over 75 minutes, 12 hours post-onset. Among the six patients with CRAO assessed in clinic, three experienced improvement in VA by two or more gradations (Snellen lines); three improved by one gradation. However, none achieved a final VA better than 20/300. The case of branch occlusion improved to a VA of 20/20. All patients had residual monocular field defects.Conclusion:Our findings reveal a limited benefit for intra-arterial tPA compared to the rate of spontaneous improvement and conventional forms of therapy for retinal artery occlusion.
One-class support vector machines with a bias constraint and its application in system reliability prediction
Authors: Hu Zhengwei , Hu Zhangli , Du Xiaoping 
Published: May 2019

AbstractSupport vector machine (SVM) methods are widely used for classification and regression analysis. In many engineering applications, only one class of data is available, and then one-class SVM methods are employed. In reliability applications, the one-class data may be failure data since the data are recorded during reliability experiments when only failures occur. Different from the problems handled by existing one-class SVM methods, there is a bias constraint in the SVM model in this work and the constraint comes from the probability of failure estimated from the failure data. In this study, a new one-class SVM regression method is proposed to accommodate the bias constraint. The one class of failure data is maximally separated from a hypersphere whose radius is determined by the known probability of failure. The proposed SVM method generates regression models that directly link the states of failure modes with design variables, and this makes it possible to obtain the joint probability density of all the component states of an engineering system, resulting in a more accurate prediction of system reliability during the design stage. Three examples are given to demonstrate the effectiveness of the new one-class SVM method.
Evaluating sub-typing methods for pathogenicYersinia enterocoliticato support outbreak investigations in New Zealand
Authors: Strydom H. , Wang J. , Paine S. , Dyet K. , Cullen K. , Wright J. 
Published: May 2019

AbstractIncidence of human yersiniosis in New Zealand has increased between 2013 and 2017. For surveillance and outbreak investigations it is essential that an appropriate level of discrimination between pathogenicYersinia enterocoliticaisolates is provided, in order to support epidemiological linking of connected cases. Subtyping of 227Y. enterocoliticaisolates was performed using a range of different typing methods, including biotyping, serotyping and seven loci multiple-locus variable-number tandem-repeat analysis (MLVA). In addition, core genome single-nucleotide polymorphism (core SNP) analysis and multi-locus sequence typing were performed on a subset of 69 isolates. Sixty-seven different MLVA types were identified. One MLVA profile was associated with an outbreak in the Bay of Plenty region, supported by epidemiological data. Core SNP analysis showed that all the outbreak-related isolates clustered together. The subtyping and epidemiological evidence suggests that the outbreak of yersiniosis in the Bay of Plenty region between October and December 2016 could be attributed to a point source. However, subtyping results further suggest that the same clone was isolated from several regions between August 2016 and March 2017. Core SNP analysis and MLVA typing failed to differentiate betweenY. enterocoliticabiotype 2 and biotype 3. For this reason, we propose that these biotypes should be reported as a single type namely:Y. enterocoliticabiotype 2/3 and that the serotype should be prioritised as an indicator of prevalence.