bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Blood Concentrations of Complement Split Product iC3b and Serum C3 With Systemic Lupus Erythematosus Disease Activity
Authors: Kim Alfred H. J. , Strand Vibeke , Sen Deepali P. , Fu Qiang , Mathis Nancy L. , Schmidt Martin J. , Bruchas Robin R. , Staten Nick R. , Olson Paul K. , Stiening Chad M. , Atkinson John P. 
Published: October 2018

ObjectiveTo examine correlations between blood levels of complement split product iC3b and serum component C3 with clinically meaningful changes in disease activity in patients with systemic lupus erythematosus (SLE).MethodsA total of 159 consecutive patients with SLE, diagnosed according to the American College of Rheumatology or Systemic Lupus International Collaborating Clinics classification criteria, were enrolled in CASTLE (Complement Activation Signatures in Systemic Lupus Erythematosus), a prospective observational study. Patients with 1–7 study visits were included in this longitudinal analysis. In addition, 48 healthy volunteers were enrolled to establish a normal reference value for the ratio of blood iC3b to serum C3 concentrations. Serum C3 and C4 levels were measured by nephelometry, and blood iC3b levels were measured by a lateral flow assay. SLE disease activity was monitored with the Responder Index 50 instrument of the SLE Disease Activity Index 2000.ResultsRelative changes in the iC3b:C3 ratio, levels of anti–double‐stranded DNA (anti‐dsDNA) antibodies, and use of a supraphysiologic dose of prednisone (>7.5 mg/day) each independently correlated with SLE disease activity, as determined in multilevel multiple logistic regression analyses. Only the iC3b:C3 ratio was significantly associated with clinically meaningful improvements in disease activity among patients with SLE who were receiving a supraphysiologic dose of prednisone. The iC3b:C3 ratio outperformed C3 and C4 levels with regard to discriminating active SLE from inactive SLE, and major flares from no disease activity. The iC3:C3 ratio, anti‐dsDNA antibody levels, erythrocyte sedimentation rate, and use of a supraphysiologic prednisone dose were each independently associated with the presence of lupus nephritis, whereas none of these measures was associated with SLE rash. The association of the iC3b:C3 ratio with lupus nephritis was independent of other observed clinical manifestations.ConclusionThe ratio of blood iC3b to serum C3 concentrations correlates with the extent of SLE disease activity and with clinically meaningful changes in disease activity in patients with SLE. Furthermore, the iC3b:C3 ratio may discriminate between active and inactive SLE, and between major flares and no active disease.
Central Sensitization in Knee Osteoarthritis: Relating Presurgical Brainstem Neuroimaging and PainDETECT‐Based Patient Stratification to Arthroplasty Outcome
Authors: Soni Anushka , Wanigasekera Vishvarani , Mezue Melvin , Cooper Cyrus , Javaid Muhammad K. , Price Andrew J. , Tracey Irene 
Published: October 2018

ObjectiveThe neural mechanisms of pain in knee osteoarthritis (OA) are not fully understood, and some patients have neuropathic‐like pain associated with central sensitization. To address this, we undertook the present study in order to identify central sensitization using neuroimaging and PainDETECT and to relate it to postarthroplasty outcome.MethodsPatients awaiting arthroplasty underwent quantitative sensory testing, psychological assessment, and functional magnetic resonance imaging (fMRI). Neuroimaging (fMRI) was conducted during punctate stimulation (n = 24) and cold stimulation (n = 20) to the affected knee. The postoperative outcome was measured using the Oxford Knee Score, patient‐reported moderate‐to‐severe long‐term pain postarthroplasty, and a range of pain‐related questionnaires.ResultsPatients with neuropathic‐like pain presurgery (identified using PainDETECT; n = 14) reported significantly higher pain in response to punctate stimuli and cold stimuli near the affected joint (P < 0.05). Neural activity in these patients, compared to those without neuropathic‐like pain, was significantly lower in the rostral anterior cingulate cortex (P < 0.05) and higher in the rostral ventromedial medulla (RVM) during punctate stimulation (P < 0.05), with significant functional connectivity between these two areas (r = 0.49, P = 0.018). Preoperative neuropathic‐like pain and higher neural activity in the RVM were associated with moderate‐to‐severe long‐term pain after arthroplasty (P = 0.0356).ConclusionThe psychophysical and neuroimaging data suggest that a subset of OA patients have centrally mediated pain sensitization. This was likely due to supraspinally mediated reductions in inhibition and increases in facilitation of nociceptive signaling, and was associated with a worse outcome following arthroplasty. The neurobiologic confirmation of central sensitization in patients with features of neuropathic pain, identified using PainDETECT, provides further support for the investigation of such bedside measures for patient stratification, to better predict postsurgical outcomes.
Energieverständnis im Biologieunterricht
Authors: Trauschke Mathias 
Published: October 2018

ZusammenfassungOhne Fleischerzeugung würde die verfügbare Ackerfläche für die Welternährung reichen. Ursächlich dafür sind Ineffizienzen in der Fleischproduktion wie energetische Analysen von Lebensmittelketten zeigen. Nutzte man stattdessen Ernteerzeugnisse direkt zur Produktion von Nahrungsmitteln, ließen sich weltweit Mangel‐ und Unterernährung eindämmen. Dieses gesellschaftlich relevante Thema ist auch für den Biologieunterricht interessant. Allerdings ist das Verständnis des Energiebegriffs im biologischen Kontext mit Schwierigkeiten behaftet, da Lernende Energie oftmals als eine substanzartige Entität (miss)verstehen und auch Schul‐ und Lehrbücher einen solchen unangemessenen Energiebegriff repräsentieren. Zur energetischen Analyse von Lebensmittelketten ist es hingegen notwendig, Energie als Bilanzierungsgröße zu verstehen. Flussdiagramme vom Sankey‐Typ bieten die Möglichkeit, den abstrakten Energiebegriff für Lernende verständlich zu repräsentieren und so den kognitiven Zugang zu bildungsrelevanten Aspekten globaler Nahrungsversorgung zu ermöglichen.
Nachrichten des Verbandes Biologie, Biowissenschaften & Biomedizin in Deutschland
Authors: Specht Sabine 
Published: October 2018

Wissenschaft & GesellschaftTierversuche in der biomedizinischen ForschungUrteil des Europäischen Gerichtshofes zu Genome EditingSchulbiologieLandesverbändeBaden‐WürttembergNRW
Feasibility study of combined dynamic imaging and lymphaticovenous anastomosis surgery for breast cancer-related lymphoedema
Authors: Khan A A , Hernan I , Adamthwaite J A , Ramsey K W D 
Published: October 2018

Abstract

Background
Breast cancer-related lymphoedema (BCRL) presents a significant healthcare burden and adversely affects quality of life of breast cancer survivors. A prospective feasibility study was performed on lymphaticovenous anastomosis (LVA) for the treatment of BCRL.

Methods
Patients with BCRL underwent near-infrared spectroscopy with indocyanine green lymphatic mapping to identify suitable lymphatic channels for LVA. End-to-end anastomoses to subdermal venules were performed and patients recommenced compression garment therapy (CGT) after surgery. Volumetric assessment of the affected limb was performed at regular intervals using infrared perometry to calculate the excess volume reduction.

Results
Over a 24-month interval, 27 patients with BCRL underwent LVA. The mean duration of lymphoedema was 3·5 (range 0·5–18) years, and the mean number of LVAs performed was 3 (range 2–5). Twenty-four of the 27 patients completed 12-month follow-up. Patients exhibited three patterns of volumetric response following LVA: sustained response (16 patients), transient response (5) or no response (6). Sustained responders showed an excess volume reduction of −33·2 per cent at 12 months, and this correlated positively with the number of LVAs performed (r = −0·56, P = 0·034). Overall, ten patients were able to downgrade CGT after surgery, and two patients were CGT-free at 12 months.

Conclusion
LVA resulted in a sustained volume reduction in selected patients and may offset the burden of CGT. Further work is required to identify biomarkers that predict a favourable response to LVA surgery.
Therapeutic change in young people—A qualitative investigation of client and therapist perspectives
Authors: Donald Ian N. , Carey Timothy A. , Rickwood Debra J. 
Published: October 2018

AbstractBackgroundYoung people's vulnerability to developing mental illness makes it important to understand therapeutic change, integrating the views of both young people and their therapists, to improve treatment effectiveness. To date, qualitative research aiming to triangulate experiences of therapeutic change in young people has not clearly differentiated the unique perspectives of clients and therapists. Instead both groups’ perspectives have been analysed together, which may be phenomenologically problematic considering clients are the site of change, while therapists observe it.AimsThis study explored experiences of therapeutic change from the perspectives of young people and their therapists. Three clients aged 17–19 years, and their two therapists, participated in semi‐structured interviews post‐therapy.MethodsSix themes were identified for clients: facing problems alone; how the therapeutic space was used; change characteristics; partial changes; the role of context in change; and growing into the new self. Four themes were identified for therapists: problematic experiences; change processes; therapist‐related change process; and change qualities.ResultsSix themes were identified for clients: facing problems alone; how the therapeutic space was used; change characteristics; partial changes; the role of context in change; and growing into the new self. Four themes were identified for therapists: problematic experiences; change processes; therapist‐related change process; and change qualities.ConclusionsCongruent with adult research, common and specific factors was important to change. Contextual and relational factors were particularly important for young people, who described that isolation caused by their problems caused distress beyond the impact of symptoms. Therapeutic change was facilitated through responsive therapy practices that led to developmentally normal levels of activity and changes in selfhood. Participants accepted a good‐enough level of recovery which was anticipated to continue post‐therapy.
Biocatalytical Asymmetric Sulfoxidation by Identifying Cytochrome P450 from Parvibaculum Lavamentivorans DS‐1
Authors: Wu Kailin , Tang Linchao , Cui Haibo , Wan Nanwei , Liu Ziyan , Wang Zhongqiang , Zhang Shimin , Cui Baodong , Han Wenyong , Chen Yongzheng 
Published: October 2018

AbstractCytochrome P450 monooxygenases (P450s) catalyzed asymmetric sulfoxidation represents a green route for the synthesis of valuable enantiopure sulfoxides, which are potentially interesting synthons in synthetic and pharmaceutical chemistry. Here the potential P450 and redox partner genes from Parvibaculum lavamentivorans DS‐1 are screened and co‐expressed in Escherichia coli host to construct twenty recombinant P450 strains. By testing the whole‐cell biooxidation of thioanisole, P450PL2 (CYP278A4) and P450PL7 (CYP108G3) are identified with excellent S enantioselectivity while P450PL1 (CYP111B1) and P450PL9 (CYP153A26) exhibit the complementary R enantioselectivity. Asymmetric sulfoxidation of sulfides 1 a–1 m is further investigated using the recombinant E. coli strain P450PL2‐2 based on the optimal conditions, producing the corresponding enantioenriched sulfoxides with up to 82 % isolated yield and 99 % ee.
Understanding Electrochemical Stability and Lithium Ion‐Dominant Transport in Concentrated Poly(ethylene carbonate) Electrolyte
Authors: Kimura Kento , Tominaga Yoichi 
Published: October 2018

AbstractIon‐conductive solid polymer electrolytes (SPEs) are important materials for implementing safer energy storage. In the present study we show that a concentrated SPE composed of poly(ethylene carbonate) (PEC) and lithium bis(fluorosulfonyl)imide (LiFSI) has high oxidation tolerance and prevents aluminum corrosion. These properties enable cycling of a LiMn2O4 cell charged above 4 V. Battery operation above 4 V is difficult for conventional polyether electrolytes because of their poor electrochemical stability. Mechanistic studies imply that an aggregated solvation structure, in which a large portion of the carbonyl groups interact with Li ions, is correlated with the enhanced electrochemical stability. The studies suggest that relatively rigid structure of PEC induces an increase in conductivity with increasing salt concentration. The increase in conductivity enables concentrated electrolyte with reasonable conductivity and high Li transference number. The present study reveals the clear potential of concentrated SPEs based on unexplored polymers having relatively high glass transition temperature for use in high‐voltage Li batteries.
Designing a Copper‐ and Silver‐Sulfide Composite with Co3O4 for High‐Performance Electrochemical Supercapacitors
Authors: Pawar Sachin A. , Patil Dipali S. , Shin Jae Cheol 
Published: October 2018

AbstractElectronic devices have driven modern lifestyle demands for efficient and environmentally friendly energy storage appliances. This paper reports the simple design of an efficient, cost‐effective pseudocapacitor electrode based on copper sulfide (Cu2S) and silver sulfide (Ag2S) composites with cobalt oxide (Co3O4) for high‐performance energy storage devices. A simple one‐pot approach was used to synthesize hexagonal sheets of Co3O4 and a successive ionic layer adsorption and reaction method was exploited to coat these sheets with nanostructures of Cu2S and Ag2S. The resulting three‐dimensional (3D) Co3O4/Cu2S and Co3O4/Ag2S composite electrode contained a high loading of Cu2S and Ag2S nanoparticles (metal sulfides) on the hexagonal sheets of Co3O4 (metal oxide), which offers a highly conductive network assisting in rapid charge‐transfer reactions and reduces the internal resistance of the electrode. A symmetrical supercapacitor based on Co3O4/Cu2S//Co3O4/Cu2S showed a high areal capacitance of 1080 mF cm−2, which was significantly higher than that of the Co3O4/Ag2S//Co3O4/Ag2S symmetric supercapacitor (645 mF cm−2). These results show that the Co3O4/Cu2S composite heterostructure is a promising electrode architecture for high performance pseudocapacitors.
Bismuth Oxychloride Nanoplatelets by Breakdown Anodization
Authors: Sopha Hanna , Spotz Zdenek , Michalicka Jan , Hromadko Ludek , Bulanek Roman , Wagner Tomas , Macak Jan M. 
Published: October 2018

AbstractHerein, the synthesis of BiOCl nanoplatelets of various dimensions is demonstrated. These materials were prepared by anodic oxidation of Bi ingots in diluted HCl under dielectric breakdown conditions, triggered by a sufficiently high anodic field. Additionally, it is shown that the use of several other common diluted acids (HNO3, H2SO4, lactic acid) resulted in the formation of various different nanostructures. The addition of NH4F to the acidic electrolytes accelerated the growth rate resulting in bismuth‐based nanostructures with comparably smaller dimensions and an enormous volume expansion observed during the growth. On the other hand, the addition of lactic acid to the acidic electrolytes decelerated the oxide growth rate. The resulting nanostructures were characterized using SEM, XRD and TEM. BiOCl nanoplatelets received by anodization in 1 M HCl were successfully employed for the photocatalytic decomposition of methylene blue dye and showed a superior performance compared to commercially available BiOCl powder with a similar crystalline structure, confirming its potential as a visible light photocatalyst.
Weight loss and breast cancer incidence in postmenopausal women
Authors: Chlebowski Rowan T. , Luo Juhua , Anderson Garnet L. , Barrington Wendy , Reding Kerryn , Simon Michael S. , Manson JoAnn E. , Rohan Thomas E. , Wactawski‐Wende Jean , Lane Dorothy , Strickler Howard , Mosaver‐Rahmani Yasmin , Freudenheim Jo L. , Saquib Nazmus , Stefanick Marcia L. 
Published: October 2018

BackgroundAlthough obesity is an established risk factor for postmenopausal breast cancer, the results of weight loss and breast cancer studies are inconsistent. Therefore, we evaluated associations between weight change and breast cancer risk in postmenopausal women in the Women’s Health Initiative Observational Study.MethodsPostmenopausal women (n = 61,335) who had no prior breast cancer and a normal mammogram had body weight and height measured and body mass index (BMI) calculated at baseline and year 3. Weight change at year 3 was categorized as stable (<5%), loss (≥5%), or gain (≥5%) with further assessment of weight loss intentionality by self‐report. Multivariable Cox proportional hazard regression models were used to evaluate relationships between weight change and subsequent breast cancer incidence.ResultsDuring a mean follow‐up of 11.4 years with 3061 incident breast cancers, women with weight loss (n = 8175) had a significantly lower risk of breast cancer compared with women whose weight remained stable (n = 41,139) (hazard ratio [HR], 0.88; 95% confidence interval [CI], 0.78‐0.98; P = .02) with no interaction by BMI. Adjustment for mammography did not alter findings (HR, 0.88; 95% CI, 0.78‐0.99) with no significant difference by weight loss intentionality. Weight gain (≥5%) (n = 12,021) was not associated with breast cancer risk (HR, 1.02; 95% CI, 0.93‐1.11) but was associated with higher triple‐negative breast cancer incidence (HR, 1.54; 95% CI, 1.16‐2.05).ConclusionsPostmenopausal women who lose weight have lower breast cancer risk than those with stable weight. These findings suggest that postmenopausal women who lose weight may reduce their breast cancer risk.
Promoting effects of color‐text congruence in banner advertising
Authors: Zhang Tengxiao , Bao Chuting , Xiao Chunqu 
Published: October 2018

AbstractPrevious studies have found that the congruence among elements in an advertisement can enhance advertising efficiency. However, the influence of congruence between color and text, two important elements of ads, has not been directly investigated. The present study aims to investigate how color‐text congruence affects advertising effectiveness in capturing attention and generating favorable attitudes. A total of 94 students from a college in China participated in an experiment using a within‐subjects design. Participants were presented with ads either matched in color and text (congruent condition) or not (incongruent condition). Attention and attitudes were measured by eye‐tracking and subjective rating, respectively. The results demonstrated that participants paid more attention and gave more positive ratings to ads in the congruent condition than those in the incongruent condition. In addition, the present study separately investigates the concrete versus abstract meanings of colors. This distinction bridges complementary theories in contemporary color psychology.
Color pattern recognition for yarn‐dyed fabrics
Authors: Zheng Dejun , Wang Peng , Zhou Jian , Ho Kai‐chiu 
Published: October 2018

AbstractIn the process of designing and analyzing the yarn‐dyed fabric, the yarn color pattern has an important effect on the appearance of the fabric. An automatic color pattern recognition method for yarn‐dyed fabric is proposed in this study. The proposed method uses the fabric images obtained from a high‐resolution digital camera image acquisition system. The local statistical texture features are used for yarn texture segmentation. The yarn color classification problem is then formulated in a research framework of multiregion fuzzy segmentation, which can be added auxiliary variables and solved efficiently by the fast dual projection algorithm. The color values of the yarn crossing points are calculated by the yarn color classification results. The locations of the yarn crossing points are detected by a lightness gradient projection method. Different kinds of fabrics are tested in the experiments. Experiments on 14 actual fabrics show that the approach proposed in this study is effective for classifying yarn color and extracting the yarn color pattern in yarn‐dyed fabric.
Autofluorescence lifetime imaging of cellular metabolism: Sensitivity toward cell density, pH, intracellular, and intercellular heterogeneity
Authors: Chacko Jenu V. , Eliceiri Kevin W. 
Published: October 2018

AbstractAutofluorescence imaging (AFI) has greatly accelerated in the last decade, way past its origins in detecting endogenous signals in biological tissues to identify differences between samples. There are many endogenous fluorescence sources of contrast but the most robust and widely utilized have been those associated with metabolism. The intrinsically fluorescent metabolic cofactors nicotinamide adenine dinucleotide (NAD+/NADH) and flavin adenine dinucleotide (FAD/FADH2) have been utilized in a number of AFI applications including basic research, clinical, and pharmaceutical studies. Fluorescence lifetime imaging microscopy (FLIM) has emerged as one of the more powerful AFI tools for NADH and FAD characterization due to its unique ability to noninvasively detect metabolite bound and free states and quantitate cellular redox ratio. However, despite this widespread biological use, many standardization methods are still needed to extend FLIM‐based AFI into a fully robust research and clinical diagnostic tools. FLIM is sensitive to a wide range of factors in the fluorophore microenvironment, and there are a number of analysis variables as well. To this end, there has been an emphasis on developing imaging standards and ways to make the image acquisition and analysis more consistent. However, biological conditions during FLIM‐based AFI imaging are rarely considered as key sources of FLIM variability. Here, we present several experimental factors with supporting data of the cellular microenvironment such as confluency, pH, inter‐/intracellular heterogeneity, and choice of cell line that need to be considered for accurate quantitative FLIM‐based AFI measurement of cellular metabolism. © 2018 International Society for Advancement of Cytometry
Expression of neutrophil surface markers in icteric neonates before and after phototherapy
Authors: Faulhaber Fabrízia Rennó Sodero , Faulhaber Gustavo Adolpho Moreira , Marcondes Natália Aydos , Procianoy Renato Soibelmann , Silveira Rita C. 
Published: October 2018

BackgroundJaundice due to indirect hyperbilirubinemia affects more than 60% of neonates and phototherapy is the treatment for severe types. There are no previous studies evaluating the effect of phototherapy on the function of neonates neutrophils. The aim of this study was to assess and compare the function of neutrophils by measuring the expression of neutrophils main surface markers in icteric neonates before and after phototherapy.MethodsNeonates at a gestational age ≥35 weeks and birth weight ≥2,000 g who met the American Academy of Pediatrics criteria for phototherapy were included. Flow cytometry evaluation of the mean fluorescence intensities of CD10, CD11b, CD11c, CD15, CD16, CD18, CD62L, CD64, and CD66acde was performed before and 24 h after the initiation of phototherapy.ResultsTwenty‐five neonates at a mean age of 53 h of life were included in the study with a mean bilirubin level of 13.60 ± 2.85 mg/dL. There was no statistical difference in the expression of CD11b, CD15, CD18, CD62L, and CD64 or in the percentage of neutrophils before and after 24 h of phototherapy. There was an increase in the expression of CD10 and CD16 and a decrease in the expression of CD11c and CD66acde after 24 h of phototherapy.ConclusionsNewborns submitted to phototherapy had an increase in the expression of CD10 and CD16 and a decreased in the expression of CD11c and CD66acde after 24 h of treatment, which may be related to an anti‐inflammatory effect of phototherapy. © 2018 International Clinical Cytometry Society