bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seed‐dispersal networks are more specialized in the Neotropics than in the Afrotropics
Authors: Dugger Phillip J. , Blendinger Pedro G. , Böhning‐Gaese Katrin , Chama Lackson , Correia Marta , Dehling D. Matthias , Emer Carine , Farwig Nina , Fricke Evan C. , Galetti Mauro , García Daniel , Grass Ingo , Heleno Ruben , Jacomassa Fábio A. F. , Moraes Suelen , Moran Catherine , Muñoz Marcia C. , Neuschulz Eike Lena , Nowak Larissa , Piratelli Augusto , Pizo Marco A. , Quitián Marta , Rogers Haldre S. , Ruggera Román A. , Saavedra Francisco , Sánchez Mariano S. , Sánchez Rocío , Santillán Vinicio , Schabo Dana G. , da Silva Fernanda Ribeiro , Timóteo Sérgio , Traveset Anna , Vollstädt Maximilian G. R. , Schleuning Matthias 
Published: November 2018

AbstractAimBiogeographical comparisons of interaction networks help to elucidate differences in ecological communities and ecosystem functioning at large scales. Neotropical ecosystems have higher diversity and a different composition of frugivores and fleshy‐fruited plants compared with Afrotropical systems, but a lack of intercontinental comparisons limits understanding of (a) whether plant–frugivore networks are structured in a similar manner, and (b) whether the same species traits define the roles of animals across continents.LocationAfrotropics and Neotropics.Time period1977–2015.TaxaFleshy‐fruited plants and frugivorous vertebrates.MethodsWe compiled a dataset comprising 17 Afrotropical and 48 Neotropical weighted seed‐dispersal networks quantifying frugivory interactions between 1,091 fleshy‐fruited plant and 665 animal species, comprising in total 8,251 interaction links between plants and animals. In addition, we compiled information on the body mass of animals and their degree of frugivory. We compared four standard network‐level metrics related to interaction diversity and specialization, accounting for differences related to sampling effort and network location. Furthermore, we tested whether animal traits (body mass, degree of frugivory) differed between continents, whether these traits were related to the network roles of species and whether these relationships varied between continents.ResultsWe found significant structural differences in networks between continents. Overall, Neotropical networks were less nested and more specialized than Afrotropical networks. At the species level, a higher body mass and degree of frugivory were associated with an increasing diversity of plant partners. Specialization of frugivores increased with the degree of frugivory, but only in the Neotropics.Main conclusionsOur findings show that Afrotropical networks have a greater overlap in plant partners among vertebrate frugivores than the more diverse networks in the Neotropics that are characterized by a greater niche partitioning. Hence, the loss of frugivore species could have stronger impacts on ecosystem functioning in the more specialized Neotropical communities compared with the more generalized Afrotropical communities.
Cardiovascular disease and diabetes in HIV‐positive and HIV‐negative gay and bisexual men over the age of 55 years in Australia: insights from the Australian Positive & Peers Longevity Evaluation Study
Authors: Puhr R , Petoumenos K , Huang R , Templeton DJ , Woolley I , Bloch M , Russell D , Law MG , Cooper DA 
Published: November 2018

ObjectivesAs HIV‐positive people age, diagnosis and management of comorbidities associated with ageing are of increasing concern. In this study, we aimed to compare the self‐reported prevalences of heart disease, stroke, thrombosis and diabetes in older Australian HIV‐positive and HIV‐negative gay and bisexual men (GBM).MethodsWe analysed data from the Australian Positive & Peers Longevity Evaluation Study (APPLES), a study of a prospectively recruited cross‐sectional sample of 228 (51.1%) HIV‐positive and 218 (48.9%) HIV‐negative GBM, aged ≥ 55 years. Regression methods were used to assess the association of HIV status with self‐reported comorbidities.ResultsOf 446 patients, 389 [200 (51.4%) HIV‐positive] reported their disease history. The reported prevalence of comorbidities was higher in the HIV‐positive group than in the HIV‐negative group: heart disease, 19.5 versus 12.2%; stroke, 7.5 versus 4.2%; thrombosis, 10.5 versus 4.2%; and diabetes, 15.0 versus 9.0%, respectively. In adjusted analyses, HIV‐positive GBM had significantly increased odds of reporting heart disease [adjusted odds ratio (aOR) 1.99; P = 0.03] and thrombosis (aOR 2.87; P = 0.01). In our analysis, HIV status was not significantly associated with either age at diagnosis of heart disease (median 53 years for HIV‐positive GBM versus 55 years for HIV‐negative GBM; P = 0.64) or 5‐year cardiovascular disease (CVD) risk estimated using the Framingham risk score.ConclusionsHIV‐positive GBM more commonly reported heart disease and thrombosis compared with their HIV‐negative peers. These results further highlight the need to understand the impact of HIV on age‐related comorbidities in GBM, to guide optimal screening and treatment strategies to reduce the risk of these comorbidities among the HIV‐positive population.
EDTA conditioning of circumpulpal dentine induces dentinogenic events in pulpotomized miniature swine teeth
Authors: Tziafas D. , Kodonas K. , Gogos C. , Tziafa C. , Papadimitriou S. 
Published: November 2018

AbstractAimTo investigate pulp responses after pulpotomy and EDTA conditioning of pulp chamber dentinal walls with or without the placement of a collagenous scaffold in the experimental model of miniature swine teeth.MethodologyForty‐two fully developed permanent premolars and molars of healthy miniature swines were used. After preparation of pulp exposures through Class I cavities, the tissue of the pulp chamber was completely removed. The haemorrhage was controlled, and the root pulp was protected using a polyurethane film. The circumpulpal pulp chamber dentine was treated for 3 min with normal saline (group 1), or 17% EDTA solution (groups 2 and 3). The film was removed, and the pulp chamber cavities were left empty (groups 1 and 2), or filled with swine collagenous sponge (group 3). The access cavities were restored with a Teflon disc and glass ionomer. Teeth were evaluated histo‐morphologically after 10 weeks. Data were compared using the nonparametric Fisher's exact test.ResultsTeeth after treatment of dentine with saline (group 1) were associated with no or only traces of hard tissue formation along the root canal walls. Atubular tertiary dentine deposition in the form of matrix deposition along root canal walls, or dentine bridge formation at the orifice of root canals or complete pulp canal obliteration, was found after treatment of dentine with EDTA in both experiments (groups 2 and 3). Significantly different types of mineralization in the root canals of groups 2 and 3 were seen (P = 0.001). Tissue changes in the pulp cavity, characterized by soft tissue growth and osteodentine or atubular tertiary dentine formation, were only seen after EDTA conditioning of dentine, in 6.2% of the teeth without scaffold and 64.7% of the teeth with scaffold application. Newly deposited mineralized matrix in the pulp chamber was always in continuation with hard tissue deposited in the root canals.ConclusionsThe EDTA conditioning of pulp cavity dentinal walls after pulpotomy induced dentinogenic events in the root pulp. Application of collagenous scaffold in the pulp chamber enhanced soft tissue growth and mineralized tissue formation along the treated circumpulpal dentine.
Recovery of residual nutrients from agri‐food byproducts using a combination of solid‐state fermentation and insect rearing
Authors: Kuttiyatveetil Jijin R. A. , Mitra Pranabendu , Goldin Darren , Nickerson Michael T. , Tanaka Takuji 
Published: November 2018

SummaryThe present research develops an innovative nutrient recovery strategy for capturing of un‐utilised nutrients from agri‐food byproducts using a combination of solid state fermentation (SSF) and insect rearing. SSF of borage and flaxseed meals were performed using GRAS organisms to release indigenous nutrients and to produce additional nutrients. Proximate analysis of the SSF‐meals showed increases in both lipid and protein contents. Black soldier fly larvae (BSFL) were then grown on these SSF‐meals for 12 day. The meals fermented singularly with Lactobacillus plantarum or Aspergillus niger displayed up to 30% enhancement in BSFL biomass, and dual fermentation with L. plantarum and Aspergillus oryzae resulted in an additional 10% enhancement. These examinations showed SFL use over‐90% of proteins and lipids in SSF‐meals. The results indicate that fermentation of these low‐value meals can boost the efficacy of larval growth and the recovery of nutrients from agricultural byproducts as larval biomass.
Recent Developments in Dealing with Item Non‐response in Surveys: A Critical Review
Authors: Chen Sixia , Haziza David 
Published: November 2018

SummaryThe most common way for treating item non‐response in surveys is to construct one or more replacement values to fill in for a missing value. This process is known as imputation. We distinguish single from multiple imputation. Single imputation consists of replacing a missing value by a single replacement value, whereas multiple imputation uses two or more replacement values. This article reviews various imputation procedures used in National Statistical Offices as well as the properties of point and variance estimators in the presence of imputed survey data. It also provides the reader with newer developments in the field.
Histories of Change in Art and Design Education in Ireland: Towards Reform: the Evolving Trajectory of Art Education
Authors: Jordan Dervil , O'Donoghue Helen 
Published: November 2018

AbstractThe serious need for reform of the Leaving Certificate Art Curriculum in Ireland sets the context for this article, which tracks the social and political factors that shaped the emergence of art education in Ireland before and after the formation of the Free State. This historical framing creates the background for the training of art teachers over three paradigms of art teacher education – the Vocational, Expressivist and Contextual. Based on doctoral research, it examines the experiences of a group of art teachers trained within these paradigms. In light of current curriculum reform, it asks the question ‘Can art education can move from a modernist skills‐based paradigm, to become a vehicle for transformation, social engagement and citizenship within a revised Leaving Certificate Art curriculum’? It proposes that the contemporary art museum can be a transformative site for change within an art curriculum.
Is success in detection of Agrilus planipennis related to forest edges?
Authors: Cladas Karen Lynn , Bal Tara Lee , Storer Andrew John 
Published: November 2018

AbstractThe emerald ash borer (Agrilus planipennis) is a destructive invasive beetle that has caused mortality of millions of Fraxinus spp. trees in North America. The extended interval between insect establishment, detection and management has allowed this pest to spread over large parts of North America. Artificial purple canopy traps are currently used in national detection surveys for this beetle. The effectiveness of purple canopy traps at low‐to‐moderate A. planipennis population densities in relation to road proximity was evaluated in 2013 and 2014. Transects of traps were established at set distances from roads in northern Michigan near an isolated A. planipennis infestation. It was hypothesized that trap effectiveness is influenced by road proximity, and that traps placed closer to roads were more likely to detect A. planipennis. A significant relationship was established between the mean number of A. planipennis captured on traps and road proximity (nearness), while no significant relationship was observed between detection success and road proximity. These findings suggest establishing traps further from roads (which can be less economically efficient) provides no greater likelihood of detection than establishing traps on or near the road edge. Basal area of non‐ash and ash species, beetle population density, vigour rating, ash tree species and sampling duration were shown to significantly influence the number of A. planipennis captured on traps.
Protist Biodiversity and Biogeography in Lakes From Four Brazilian River–Floodplain Systems
Authors: Lentendu Guillaume , Buosi Paulo Roberto Bressan , Cabral Adalgisa Fernada , Trevizan Segóvia Bianca , Ramos Meira Bianca , Lansac‐Tôha Fernando Miranda , Velho Luiz Felipe Machado , Ritter Camila D. , Dunthorn Micah 
Published: November 2018

AbstractThe biodiversity and biogeography of protists inhabiting many ecosystems have been intensely studied using different sequencing approaches, but tropical ecosystems are relatively under‐studied. Here, we sampled planktonic waters from 32 lakes associated with four different river–floodplains systems in Brazil, and sequenced the DNA using a metabarcoding approach with general eukaryotic primers. The lakes were dominated by the largely free‐living Discoba (mostly the Euglenida), Ciliophora, and Ochrophyta. There was low community similarity between lakes even within the same river–floodplain. The protists inhabiting these floodplain systems comprise part of the large and relatively undiscovered diversity in the tropics.
Detection and quantification of Aeromonas schubertii in Channa maculata by TaqMan MGB probe fluorescence real‐time quantitative PCR
Authors: Liu Chun , Guo Yanming M. , Cao Jizhen Z. , Zhang De‐Feng , Chang Ou‐Qin , Li Kaibin , Wang Fang , Shi Cun‐Bin , Jiang Lan , Wang Qing , Lin Li 
Published: November 2018

AbstractAeromonas schubertii is a major epidemiological agent that threatens cultured snakeheads (Channidae) and has caused great economic losses in fish‐farming industries in China in recent years. In present study, a specific TaqMan minor groove binder (MGB) probe fluorescence real‐time quantitative PCR (qPCR) assay was developed to rapidly detect and quantify A. schubertii. A pair of qPCR primers and a TaqMan MGB probe were selected from the rpoD gene, which were shown to be specific for A. schubertii. A high correlation coefficient (R2 = 0.9998) in a standard curve with a 103% efficiency was obtained. Moreover, the qPCR method's detection limit was as low as 18 copies/μl, which was 100 times more sensitive than that of conventional PCR. The detection results for the A. schubertii in pond water and fish tissue were consistent with those of the viable counts. Bacterial load changes detected by qPCR in different tissues of snakeheads infected with A. schubertii showed that the gills and intestines may be the entry for A. schubertii, and the spleen and kidney are major sites for A. schubertii replication. The established method in present study should be a useful tool for the early surveillance and quantitation of A. schubertii.
Analysis of Bacillus cereus cell viability, sublethal injury, and death induced by mild thermal treatment
Authors: Lv Ruiling , Wang Danli , Zou Mingming , Wang Wenjun , Ma Xiaobin , Chen Weijun , Zhou Jianwei , Ding Tian , Ye Xingqian , Liu Donghong 
Published: November 2018

AbstractAs the most cost‐effective tool to ensure microbial safety, thermal processing induces a large portion of sublethal injury presenting a potential hazard to food safety. Thermal treatment at 63 °C for 1 min injured 2.22 log cfu/ml totally, half of which were sublethally injured using plate counting method. After 2 min, the inactivation log of Bacillus cereus reached 1.55 while the sublethally injured log even reached 2.16. As for the sublethally injured rate of the B. cereus, it was over 65% after 0.5 min and kept ever‐increase with time. In the end, the injured rate arrived at 99.30% after 2 min processing time. Partial esterase enzyme inactivation was found after 0.5 min heat treatment with flow cytometry combining carboxyfluorescein diacetate/propidium iodide (PI) double‐staining, but B. cereus was not dyed by PI at 63 °C. Comparing with the initial protein concentration of 0.57 ± 0.02 μg/ml, the leakage of the protein was not so notable though the general trend was increasing with duration of heat. Scanning and transmission electron microscopy analysis revealed that a portion of cells morphology and structure had changed after thermal process.Practical applications
Bacillus cereus is an endospore forming pathogenic, causing foodborne illnesses and outbreaks all over the world. This research indicated that thermal treatment at 63 °C was a sublethal stress for B. cereus that a portion of cells were sublethally injured which could resume growth in suitable condition and might exist potential safety hazard if used for food pasteurization.
Evaluation of psychrophilic, mesophilic, histamine forming bacteria and biogenic amine content in the muscle of mud spiny lobster, Panulirus polyphagus (HERBST, 1793) during ice storage
Authors: Arulkumar A. , Paramasivam S. , Rameshthangam P. , Paramithiotis S. 
Published: November 2018

AbstractThe aim of the present study was to assess the evolution of psychrophilic, mesophilic and histamine forming bacteria along with the accumulation of cadaverine, histamine, putrescine, spermidine, spermine, tryptamine and tyramine during ice storage of mud spiny lobster (Panulirus polyphagus). Total mesophilic, psychrophilic and amine forming bacteria were initially enumerated at 2.62, 2.14 and 2.10 log cfu/g muscle, respectively and reached 7.07, 7.11, and 6.80 log cfu/g muscle, respectively, after 15 days of ice storage. Tryptamine, putrescine and histamine were detected from the 5th day, tyramine, spermidine, and spermine from the 10th day and cadaverine only at the 15th day of storage. Positive correlation of mesophilic and psychrophilic bacteria with tryptamine, putrescine and tyramine was detected. Species belonging to Enterobacter spp., Proteus spp., Klebsiella spp., Citrobacter spp., Photobacterium spp., Serratia spp., and Morganella morganii able to produce histamine, putrescine, cadaverine and tyramine were isolated during storage. The results obtained suggest that P. polyphagus muscle can be stored in ice for short period of time, which may reach 10 days. After the 10th day of ice storage, accumulation of biogenic amines may constitute a potential health hazard.Practical applicationsTo improve our understanding of freshness and bacterial species contribution to biogenic amines production in lobster during ice storage, knowledge of the microecosystem dynamics is necessary. Therefore, we investigated the dynamics of mesophilic, psychrophilic and histamine forming bacteria as well as the evolution of cadaverine, histamine, putrescine, spermidine, spermine, tryptamine and tyramine during ice storage of mud spiny lobster (Panulirus polyphagus). The results of the present study could be useful to assure the safety of lobster by controlling the biogenic amines content.
Comparison of three different stents for endoscopic ultrasound‐guided drainage of pancreatic fluid collection: A large retrospective study
Authors: Wang Zhijie , Zhao Shengbing , Meng Qianqian , Wang Shuling , Chen Yan , Wang Fuming , Li Qiang , Yao Jun , Du Yiqi , Jin Zhendong , Bai Yu , Li Zhaoshen , Wang Dong 
Published: November 2018

AbstractBackground and AimEndoscopic transmural drainage has been recognized as the first‐line treatment for pancreatic fluid collection (PFC). Currently, three different types of stents have been extensively applied, including double pigtail plastic stent (PS), fully covered self‐expanding metal stent (FCSEMS), and novel lumen‐apposing metal stent (LAMS). Nonetheless, limited data are available about the comparison among them in terms of their clinical outcomes and safety for endoscopic ultrasound‐guided drainage of PFC.MethodsThe current retrospective study was carried on 160 PFC patients undergoing endoscopic ultrasound‐guided drainage from 2010 to 2018 at a single tertiary care center. Patients were divided into three groups based on different drainage ways: drainage using PS, FCSEMS, or LAMS.ResultsA total of 160 PFC patients (104 male and 56 female) were analyzed in this retrospective study, including 62 patients drained with PS, 28 with FCSEMS, and 70 with LAMS. Typically, the technical success (93.5% vs 96.4% vs 94.3%, P = 1.000) and treatment success rates (84.6% vs 85.2% vs 89.2%, P = 0.763) were similar between PS, FCSEMS, and LAMS. With regard to major adverse events, four patients (FCSEMS: n = 2 and LAMS: n = 2) with pseudoaneurysms developed severe bleeding; among them, 75% (3/4) of patients were observed within 2 weeks after intervention, and two patients in LAMS group died.ConclusionsThere are no significant differences in the clinical outcomes of PFC patients treated with PS, FCSEMS, or LAMS. Nonetheless, severe (or even fatal) bleeding may occur at the early stage after metal stent placement, which should be paid particular attention to.
Inclination of the condylar long axis is not related to temporomandibular disc displacement
Authors: Amorim Marian Y. , Alves Monica G. O. , Almeida Janete D. , Montesinos Gonzalo A. P. , Costa Andre L. F. , Lopes Sergio L. P. de C. 
Published: November 2018

AbstractAimThe aim of the present study was to correlate the angle formed by inclination of the condylar long axis and median sagittal plane to the presence of internal disc derangement, as well as changes in eccentric mandibular positions.MethodsThe sample consisted of magnetic resonance images of 35 temporomandibular joint (TMJ) acquired with a 1.5T magnetic resonance imaging system with surface coils of 8.5 cm in diameter. Parasagittal images were used according to the following parameters: TMJ disc position with mouth closed, TMJ disc position with mouth open, TMJ disc function, and degrees of rotation and translation of the condyle. Next, we determined the angles between the condyle long axis and median sagittal plane by using axial images.ResultsThe results showed correlations between TMJ disc position and disc function (P = 0.009), as well as between disc position and degree of condylar rotation (P = 0.008). The main purpose of the present study was to assess the angle of the condylar long axis, with no statistical correlation being found between this variable and disc position (P = 0.787), disc function (P = 0.091), condylar translation (P = 0.808), and condylar rotation (P = 0.115).ConclusionThere was no relationship between inclination of the condyle long axis and parameters of internal derangements.