bluuebunny/crossref_metadata_2025_split huggingface dataset:
The importance of being cross-linked for the bacterial cell wall
Authors: Rani Garima , Patri Issan 
Published: March 2019

AbstractThe bacterial cell wall is primarily composed of a mesh of stiff glycan strands cross-linked by peptide bridges and is essential for safeguarding the cell. The structure of the cell wall has to be stiff enough to bear the high turgor pressure and sufficiently tough to ensure protection against failure. Here we explore the role of various design features of the cell in enhancing the toughness of the cell wall. We explain how the glycan strand length distribution and the degree of cross-linking can play a vital role in ensuring that the cell wall offers sufficient resistance to propagation of cracks. We suggest a possible mechanism by which peptide bond hydrolysis can also help mitigate this risk of failure. We also study the reinforcing effect of MreB on the cell wall and conclude that the cross-linked structure of the cell wall plays the more important role in safeguarding against mechanical failure due to cracking.
The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection
Authors: Moskovskich Anna , Goldmann Ulrich , Kartnig Felix , Lindinger Sabrina , Konecka Justyna , Fiume Giuseppe , Girardi Enrico , Superti-Furga Giulio 
Published: March 2019

AbstractHost factor requirements for different classes of viruses are still to be fully unraveled. Replication of the viral genome and synthesis of viral proteins inside the human host cell are associated with altered, often enhanced, cellular metabolism and increased demand for nutrients as well as specific metabolites. With more than 400 members listed to date in humans, solute carriers (SLCs) represent the largest family of transmembrane proteins dedicated to the transport of ions and small molecules such as amino acids, sugars and nucleotides. Consistent with their impact on cellular metabolism, several SLCs have been implicated as host factors affecting the viral life cycle and the cell response to infection. In this study, we aimed at characterizing the role of host SLCs in cell survival upon viral infection by performing unbiased genetic screens using a focused CRISPR knockout library. Genetic screens with the cytolytic vesicular stomatitis virus (VSV) showed that loss of two SLCs genes, encoding the sialic acid transporter SLC35A1/CST and the zinc transporter SLC30A1/ZnT1, affected cell survival upon infection. Further characterization of these genes pointed to a role of both transporters in the apoptotic response induced by VSV, offering new insights into the cellular response to oncolytic virus infections.
ComplexBrowser: a tool for identification and quantification of protein complexes in large scale proteomics datasets
Authors: Michalak Wojciech , Tsiamis Vasileios , Schwämmle Veit , Rogowska-Wrzesińska Adelina 
Published: March 2019

AbstractWe have developed ComplexBrowser, an open source, online platform for supervised analysis of quantitative proteomics data that focuses on protein complexes. The software uses information from CORUM and Complex Portal databases to identify protein complex components. Based on the expression changes of individual complex subunits across the proteomics experiment it calculates Complex Fold Change (CFC) factor that characterises the overall protein complex expression trend and the level of subunit co-regulation. Thus up- and down-regulated complexes can be identified. It provides interactive visualisation of protein complexes composition and expression for exploratory analysis. It also incorporates a quality control step that includes normalisation and statistical analysis based on Limma test. ComplexBrowser performance was tested on two previously published proteomics studies identifying changes in protein expression in human adenocarcinoma tissue and during activation of mouse T-cells. The analysis revealed 1519 and 332 protein complexes, of which 233 and 41 were found co-ordinately regulated in the respective studies. The adopted approach provided evidence for a shift to glucose-based metabolism and high proliferation in adenocarcinoma tissues and identification of chromatin remodelling complexes involved in mouse T-cell activation. The results correlate with the original interpretation of the experiments and also provide novel biological details about protein complexes affected. ComplexBrowser is, to our knowledge, the first tool to automate quantitative protein complex analysis for high-throughput studies, providing insights into protein complex regulation within minutes of analysis.A fully functional demo version of ComplexBrowser v1.0 is available online via http://computproteomics.bmb.sdu.dk/Apps/ComplexBrowser/The source code can be downloaded from: https://bitbucket.org/michalakw/complexbrowserHighlightsAutomated analysis of protein complexes in proteomics experimentsQuantitative measure of the coordinated changes in protein complex componentsInteractive visualisations for exploratory analysis of proteomics resultsIn briefComplexBrowser is capable of identifying protein complexes in datasets obtained from large scale quantitative proteomics experiments. It provides, in the form of the CFC factor, a quantitative measure of the coordinated changes in complex components. This facilitates assessing the overall trends in the processes governed by the identified protein complexes providing a new and complementary way of interpreting proteomics experiments.
Dissection of acute stimulus-inducible nucleosome remodeling in mammalian cells
Authors: Comoglio Federico , Simonatto Marta , Polletti Sara , Liu Xin , Smale Stephen T. , Barozzi Iros , Natoli Gioacchino 
Published: March 2019

ABSTRACTAccessibility of the genomic regulatory information is largely controlled by the nucleosome-organizing activity of transcription factors (TFs). Whereas stimulus-induced TFs bind to genomic regions that are maintained accessible by lineage-determining TFs, they also increase accessibility of thousands of cis-regulatory elements. Nucleosome remodeling events underlying such changes and their interplay with basal positioning are unknown. Here, we devised a novel quantitative framework discriminating different types of nucleosome remodeling events in micrococcal nuclease ChIP-seq datasets and used it to analyze nucleosome dynamics at stimulus-regulated cis-regulatory elements. At enhancers, remodeling preferentially affected poorly positioned nucleosomes while sparing well-positioned nucleosomes flanking the enhancer core, indicating that inducible TFs do not suffice to overrule basal nucleosomal organization maintained by lineage-determining TFs. Remodeling events appeared to be combinatorially driven by multiple TFs, with distinct TFs showing however different remodeling efficiencies. Overall, these data provide a systematic view of the impact of stimulation on nucleosome organization and genome accessibility in mammalian cells.
Injection practices in 2011-2015: A rapid review using data from the Demographic and Health Surveys (DHS)
Authors: Hayashi Tomoyuki , Hutin Yvan J-F , Bulterys Marc , Altaf Arshad , Allegranzi Benedetta 
Published: March 2019

AbstractBackgroundReuse of injection devices to give healthcare injections decreased from 39.8% to 5.5% between 2000 and 2010, but trends since 2011 have not been described. We reviewed results of Demographic and Health Surveys (DHS) to describe injection practices worldwide from 2011 to 2015.MethodsWe searched the DHS Internet site for data published on injection practices conducted in countries from 2011 to 2015, extracted information on frequency (number of healthcare injections per person in the last 12 months) and safety (proportion of syringes and needles taken from a new, unopened package). We compared gender groups and WHO regions in terms of frequency and safety. For countries with data available, we compared injection practices 2004- 2010 and 2011-2015.ResultsSince 2011, 40 of 92 countries (43%) that had DHS surveys reported on injection practices. On average, the frequency of injection was 1.64 per person per year (from 3.84 in WHO Eastern Mediterranean region to 1.18 in WHO African region). Among those, 96.1% of injections reportedly used new injection devices (from 90.2% in the WHO Eastern Mediterranean region to 98.8% in the WHO Western Pacific region). On average, women received more injections per year (1.85) than men (1.41). Among 16 (40%) countries with data up to 2010 and since 2011, 69% improved in terms of safety. The annual number of unsafe injections was reduced in 81% of countries, with the notable exception of Pakistan where the number of unsafe injections was the highest and did not decrease between 2006 and 2012.ConclusionInjection practices have continued to improve in most countries worldwide, although the Eastern Mediterranean region in particular is facing residual unsafe practices that are not improving. Further efforts are needed to completely eliminate unsafe injection practices in health care settings, including through the use of reuse-prevention devices. Despite some limitations, DHS is an easily available method to measure progress over time.
A perfect couple? Institutional theory and entrepreneurship research
Authors: Zhai Qinghua , Su Jing 
Published: March 2019

Purpose
This paper aims to evaluate the progress made in understanding the impact of multi-level institutions on entrepreneurship.

Design/methodology/approach
Based on scientific articles published between 1992 and 2017, the authors take a unique focus on both institutional theory applied and research topics of this area. Bibliometric method and systematic literature review method are used.

Findings
The results demonstrate that although institutional theory is well prepared for entrepreneurship context operating at different levels, the major knowledge foundation used predominantly focuses on macro and meso level. When it comes to research topics, entrepreneurship is often simplified as the founding of new venture, and the unique venture founding process has rarely been explored.

Originality/value
This paper is the first to provide a full picture of the multi-level institutions and their consequences on different kinds of entrepreneurial activities. The authors’evaluation of this research area also points out directions for future study.
Effects of the classification system in international SAR
Authors: Okita Yosuke , Shaw Rajib 
Published: March 2019

Purpose
The purpose of this paper is to analyse if the classification system introduced by International Search and Rescue Advisory Group (INSARAG), or INSARAG External Classification (IEC), contributes to effective international search and rescue (SAR) activities in the 2015 Nepal earthquake.

Design/methodology/approach
In addition to the data collected by Office for the Coordination of Humanitarian Affairs and the United Nations Disaster Assessment and Coordination (UNDAC) team, the data were collected by one of the authors who was deployed to Nepal as part of the UNDAC just after the earthquake. Interviews with the deployed international SAR teams and the INSARAG Secretariat were also conducted.

Findings
Although more than 50 teams have been classified in IEC, some IEC-classified teams could not utilise their full capabilities in the Nepal response. For example, they did not necessarily arrive in Nepal earlier than the non-classified teams, but it was because the affected country did not prioritise the IEC-classified teams. To save more lives by international teams, INSARAG will need to raise the awareness of IEC in receiving countries, consider the good regional balance of IEC-classified teams and facilitate strengthening local SAR capabilities through the IEC process.

Originality/value
The added value of this study is, by combining the evidence-based field reality and academic analysis, to find out the existing problems in the field and to provide tangible recommendations for further improvement of the IEC system, which will then lead to saving more lives.
Investment valuation model for sustainable infrastructure systems
Authors: Gonzalez-Ruiz Juan David , Arboleda Alejandro , Botero Sergio , Rojo Javier 
Published: March 2019

Purpose
The purpose of this paper is to develop an investment valuation model using the mezzanine debt mechanism based on blue bonds that explicitly allude to public–private partnerships (P3s) and project finance (PF). Additionally, this study proposes the financial captured value (FCV) theory for measuring how much financial value lenders may capture by becoming sponsors through financing of sustainable infrastructure systems (SIS).

Design/methodology/approach
The investment valuation model was validated through the Aguas Claras wastewater treatment plant as a case study.

Findings
The empirical results show that lenders may capture financial value by converting outstanding debt into equity shares throughout the operation and maintenance stage. Furthermore, case study results provide new insights into the implications of the debt–equity conversion ratio on the relationship between the sponsors’ internal rate of return and the FCV.

Research limitations/implications
The most significant limitation is the lack of primary and secondary information on blue bonds. Thus, robust statistical analyses to contrast results were not possible.

Practical implications
Researchers and practising professionals can improve their understanding of how mezzanine debt, P3s and PF into an investment valuation model allows financing SIS using a non-conventional financial mechanism. The recommendations will benefit both the academia as well infrastructure industry in bridging the gap between design theory and practice.

Originality/value
Sustainability components have not been addressed explicitly or combined in the financing’s structuring. Therefore, the investment valuation model could be considered a novel methodology for decision making related to financing and investment of SIS.
The role of HRD in university – community partnership
Authors: Osafo Emmanuel , Yawson Robert M. 
Published: March 2019

Purpose
This paper aims to identify ways by which the core functions of human resource development HRD can be used to enhance the university–community partnership (UCP) in lieu of the “town and gown” era. Furthermore, the paper addresses the need to extend HRD activities beyond the organization and leverage HRD to spearhead the community-development agenda through coalition building between organizations, local universities and the community.

Design/methodology/approach
Literature on UCP is reviewed and analyzed, and the need to extend HRD focus beyond the organization to include community development through coalition building is discussed. A single-case descriptive analysis to illustrate the critical role of human resource and leadership development in UCP is done.

Findings
HRD’s interest in the UCP drive is negligible. UCP presents a new frontier for HRD research and practice because there is both public and private funding that can be assessed through the right contacts and networks.

Originality/value
The need for UCP has been a subject of discussion among scholars for time immemorial. However, the collapse of the “town and gown” era has inspired greater interest in UCP. HRD scholars and practitioners can leverage the expertise in applying andragogy principles, the focus on the adult learner and community leadership development to play a crucial in the UCP drive.
Development of a user-friendly regression model to evaluate carbon emissions of office buildings design in the subtropics
Authors: Lee Pan , Chan Edwin H.W. , Qian Queena K. , Lam Patrick T.I. 
Published: March 2019

Purpose
Design teams have difficulties in assessing building carbon emissions at an early stage, as most building energy simulation tools require a detailed input of building design for estimation. The purpose of this paper is to develop a user-friendly regression model to estimate carbon emissions of the preliminary design of office buildings in the subtropics by way of example. Five sets of building design parameters, including building configuration, building envelope, design space conditions, building system configuration and occupant behaviour, are considered in this study.

Design/methodology/approach
Both EnergyPlus and Monte Carlo simulation were used to predict carbon emissions for different combinations of the design parameters. A total of 100,000 simulations were conducted to ensure a full range of simulation results. Based on the simulation results, a regression model was developed to estimate carbon emissions of office buildings based on preliminary design information.

Findings
The results show that occupant density, annual mean occupancy rate, equipment load, lighting load and chiller coefficient of performance are the top five influential parameters affecting building carbon emissions under the subtropics. Besides, the design parameters of ten office buildings were input into this user-friendly regression model for validation. The results show that the ranking of its simulated carbon emissions for these ten buildings is consistent with the original carbon emissions ranking.

Practical implications
With the use of this developed regression model, design teams can not only have a simple and quick estimation of carbon emissions based on the building design information at the conceptual stage but also explore design options by understanding the level of reduction in carbon emissions if a certain building design parameter is changed. The study also provides recommendations on building design to reduce carbon emissions of office buildings.

Originality/value
Limited research has been conducted to date to investigate how the change of building design affects carbon emissions in the subtropics where four distinct seasons lead to significant variations of outdoor temperature and relative humidity. Previous research also did not emphasise on the impact of high-rise office building designs (e.g. small building footprint, high window-to-wall ratio) on carbon emissions. This paper adds value by identifying the influential parameters affecting carbon emissions for a high-rise office building design and allows a handy estimate of building carbon emissions under the subtropical conditions. The same approach may be used for other meteorological conditions.
Crisis leadership competencies: the facility management sector in Thailand
Authors: Wisittigars Boonkiat , Siengthai Sununta 
Published: March 2019

Purpose
This paper aims to identify crisis leadership competencies in the facility management (FM) sector in Thailand.

Design/methodology/approach
The Delphi technique was used in three rounds of opinion evaluation from 24 Thai FM experts, based on which a large-scale questionnaire survey instrument was developed and administered. Of the 350 questionnaires distributed, 290 usable questionnaires were obtained (82.85 per cent response rate). Factor analysis was used to reveal important leadership competencies for managing facilities in crisis situations.

Findings
The Delphi technique identified 32 potential FM crisis leadership competencies. Of these, principal component analysis revealed 29 significant competencies. These competencies were grouped, using factor loadings, into five different competencies: emergency preparedness; crisis communication; emotional intelligence; leadership skills; and problem-solving. Emergency preparedness was found to be the most important leadership competency in FM crisis management.

Research limitations/implications
This study is limited to the experiences of FM experts in Thailand. Its empirical results can help human resource managers to develop appropriate training programs and policies for FM practitioners, as well as to help junior FM practitioners develop competencies essential for leaders in the FM sector.

Originality/value
This is a novel empirical study of leadership competencies in a growing business sector in Thailand (FM) and possibly other countries in the Asian region. Leaders in FM can benefit from recognizing the leadership competencies that are critical during crisis management.
Explaining the challenges of academic professional ethics training from the perspective of faculty members at Ardabil University of Medical Sciences: a qualitative study
Authors: Habibzadeh Shahram , Zandian Hamed , Edalatkhah Hasan , Mehrtak Mohammad 
Published: March 2019

Purpose
Education systems throughout the world seek to take the advantage of every presenting opportunity for achieving a standard level of ethical accountability and capability. The purpose of this paper is to explain the challenges of professional ethics training from the perspective of faculty members at the Ardabil University of Medical Sciences (ARUMS).

Design/methodology/approach
The present qualitative framework analysis held interviews with 21 key faculty members were selected using the purposeful and snowball method to interview from ARUMS in 2016. The duration of each interview varied between 45 and 120 min. The data obtained were analyzed in MAXQDA-10. Codes were extracted using inductive methods.

Findings
Three main themes were extracted from the interviews, namely, educational efficacy, professional interaction and equity in education along with 12 subthemes, namely, teacher’s personal capability, work in the area of expertise, regular class attendance, quality class attendance, inclusion of theoretical bases, the teacher as an ethical role model, respect for the student, encouragement of ethical behaviors, respect for the patient’s rights, fair assessment, attention to differences and positive discrimination.

Practical implications
This study results could help to improve the quality of training and ethical standards in medical education.

Originality/value
To comply with the codes of ethics in research, permission was obtained from the Ethics Committee and the participants were ensured about the confidentiality of their data and their right to withdraw from the study at any stage. Given that this project was a qualitative study, the authors did not have a moral or practical problem in the study. This study was approved with number “IR.ARUMS.FEC.1394.25” at the Ardabil University of Medical Sciences Research Ethics Council. The cooperation of participants in the study was completely voluntary; moreover, the authors obtained written consent from participants for the study.