bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel Strategy to Accelerate Bone Regeneration of Calcium Phosphate Cement by Incorporating 3D Plotted Poly(lactic‐co‐glycolic acid) Network and Bioactive Wollastonite
Authors: Qian Guowen , Fan Peirong , He Fupo , Ye Jiandong 
Published: March 2019

AbstractInefficient bone regeneration of self‐hardening calcium phosphate cement (CPC) increases the demand for interconnected macropores and osteogenesis‐stimulated substances. It remains a challenge to fabricate porous CPC with interconnected macropores while maintaining its advantages, such as plasticity. Herein, pastes containing CPC and wollastonite (WS) are infiltrated into a 3D plotted poly(lactic‐co‐glycolic acid) (PLGA) network to fabricate plastic CPC‐based composite cement (PLGA/WS/CPC). The PLGA/WS/CPC recovers the plasticity of CPC after being heated above the glass transition temperature of PLGA. The presence of the 3D PLGA network significantly increases the flexibility of CPC in prophase and generates 3D interconnected macropores in situ upon its degradation. The addition of WS is helpful to improve the attachment, proliferation, and osteogenic differentiation of mouse bone marrow stromal cells in vitro. The in vivo experimental results indicate that PLGA/WS/CPC promotes rapid angiogenesis and bone formation. Therefore, the plastic CPC‐based composite cement with a 3D PLGA network and wollastonite shows an obviously improved efficiency for repairing bone defects and is expected to facilitate the wider application of CPC in the clinic.
Ultrasound/Acidity‐Triggered and Nanoparticle‐Enabled Analgesia
Authors: Gao Xiong , Zhu Piao , Yu Luodan , Yang Liqun , Chen Yu 
Published: March 2019

AbstractLocal anesthetics have been extensively employed to treat postoperative pain, but they generally suffer from short acting duration and potential neurotoxicity under high local concentrations, which require the controlled and sustained releasing patterns of treatment drugs. In this work, it is reported, for the first time, the construction of hollow mesoporous organosilica nanoparticles (HMONs)‐based nanoplatforms for localized delivery and controlled/sustained release of loaded ropivacaine for local anesthetics, which can be repeatedly triggered by either external ultrasound irradiation or acidity triggering to release the payload, causing on‐demand and long‐lasting analgesia. Based on the in vivo mouse model of incision pain, the controlled and sustained release of ropivacaine achieves more than six hours of continuous analgesia, which is almost three times longer as compared to single free ropivacaine injection. The low neurotoxicity and high biocompatibility of HMONs for nanoparticle‐enabled analgesia are also demonstrated both in vitro and in vivo. This designed/constructed HMONs‐based nanoplatform provides a potential methodology for clinical pain management via on‐demand and long‐lasting pain relief.
Chiral N,N′‐Dioxide/Tm(OTf)3 Complex‐Catalyzed Asymmetric Bisvinylogous Mannich Reaction of Silyl Ketene Acetal with Aldimines
Authors: Zou Sijia , Li Weiwei , Fu Kai , Cao Weidi , Lin Lili , Feng Xiaoming 
Published: March 2019

AbstractAn enantioselective bisvinylogous Mannich reaction of silyl ketene acetal with aldimines has been realized by using a chiral N,N′‐dioxide/Tm(OTf)3 complex as catalyst, providing an effective method to synthesize chiral ζ‐amino‐α,β,γ,δ‐unsaturated carbonyl compounds.magnified image
Metal‐free Carbon Monoxide (CO) Capture and Utilization: Formylation of Amines
Authors: Noh Hyeong‐Wan , An Youngjoon , Lee Seulchan , Jung Jaehoon , Son Seung Uk , Jang Hye‐Young 
Published: March 2019

AbstractThe capture and utilization of CO by 1,5,7‐triazabicyclo[4.4.0]dec‐5‐ene (TBD) were performed in the absence of transition‐metal complexes. The reaction of TBD with CO afforded TBD‐CO adducts, which were converted to formylated TBD (TBD‐CHO). TBD‐CO adducts may include an interaction of CO with positively charged species based on NMR and IR analysis. In the presence of amines, CO was transferred from TBD‐CO to amines, producing formylated amines with good yields. The reaction mechanism involving TBD‐CO adducts is presented based on theoretical calculations.magnified image
Studies on the Laccase‐Catalyzed Oxidation of 4‐Hydroxy‐Chalcones
Authors: Grosso Simone , Radaelli Fabio , Fronza Giovanni , Passarella Daniele , Monti Daniela , Riva Sergio 
Published: March 2019

AbstractThe laccase‐catalyzed oxidation of a series of substituted 4‐hydroxy‐chalcones has been investigated. The main isolated dimeric products were, as expected, racemic mixtures of trans‐2,3‐dihydrobenzofuran derivatives, always co‐eluted with an additional isomeric dimer with an open structure. The two enantiomers, as well as the co‐eluted dimeric isomer could be isolated by semi‐preparative HPLC with a chiral column and were fully characterized.magnified image
Constituents of a mixed‐ploidy population of Solidago altissima differ in plasticity and predicted response to selection under simulated climate change
Authors: Zlonis Katharine J. , Etterson Julie R. 
Published: March 2019

Premise of the StudyPolyploids possess unique attributes that influence their environmental tolerance and geographic distribution. It is often unknown, however, whether cytotypes within mixed‐ploidy populations are also uniquely adapted and differ in their responses to environmental change. Here, we examine whether diploids and hexaploids from a single mixed‐ploidy population of Solidago altissima differ in plasticity and potential response to natural selection under conditions simulating climate change.MethodsClonal replicates of diploid and hexaploid genotypes were grown in a randomized split‐plot design under two temperature (+1.9°C) and two watering treatments (–13% soil moisture) implemented with open‐top passive chambers placed under rainout shelters. Physiological, phenological, morphological traits, and a fitness correlate, reproductive biomass, were measured and compared among treatments.Key ResultsDifferences in traits suggest that diploids are currently better adapted to low‐ water availability than hexaploids. Both ploidy levels had adaptive plastic responses to treatments and are predicted to respond to selection, but often for different traits. Water availability generally had a stronger effect than temperature, but for some traits the effect of water depended on temperature.ConclusionsDiploid and hexaploid S. altissima may maintain fitness in the short term through adaptive plasticity and evolution depending on which traits are important in a warmer, drier environment. Hexaploids may be at a disadvantage compared to diploids because fewer traits were heritable. Our results underscore the importance of studying combinations of climate variables that are predicted to change simultaneously.
Distinct mutation spectrum, clinical outcome and therapeutic responses of typical complex/monosomy karyotype acute myeloid leukemia carrying TP53 mutations
Authors: Leung Garret M. K. , Zhang Chunxiao , Ng Nelson K. L. , Yang Ning , Lam Stephen S. Y. , Au Chun H. , Chan Tsun L. , Ma Edmond S. K. , Tsui Sze P. , Ip Ho W. , So Jason C. C. , Ng Margaret H. L. , Cheng Kelvin C. K. , Wong Kit F. , Siu Lisa L. P. , Yip Sze F. , Lin Shek Y. , Lau June S. M. , Luk Tsan H. , Lee Harold K. K. , Lau Chi K. , Kho Bonnie , Kwong Yok L. , Leung Anskar Y. H. 
Published: March 2019

AbstractThe present study aimed to define a subtype of complex/monosomal karyotype (CK/MK) acute myeloid leukemia (AML) by its distinct clinical features, p53 signaling and responses to p53 targeting agents. Ninety‐eight young adults (range: 21‐60 years; median: 49 years) with CK/MK AML were studied. They received standard induction, consolidation and allogeneic hematopoietic stem cell transplantation from siblings or matched unrelated donors if available. Chromosomal abnormalities most commonly affected chromosome 5 (30%), 7 (22%) and 17 (21%). Next generation sequencing of a 54‐myeloid gene panel were available in 76 patients. Tumor protein 53 (TP53) mutations were most common (49%) and associated with the presence of −5/5q‐ (P < .001) and −17/17p‐ (P < .001), but not −7/7q‐ (P = .370). This “typical” CK/MK AML subtype was associated with significantly lower presenting white cell counts, higher number of karyotypic abnormalities, and inferior leukemia‐free and overall survivals, compared with CK/MK AML without the typical features. Blood or bone marrow samples from typical CK/MK AML patients showed defective p53 signaling upon induction by etoposide. In vitro drug sensitivity analysis showed that they were sensitive to APR‐246 that targeted mutant p53, but resistant to MDM2 antagonist MI‐77301. Novel therapeutic strategies targeting TP53 mutations in CK/MK AML should be developed and tested in clinical trials.
Transient Incomplete Separation Facilitates Finding Accurate Equilibrium Dissociation Constant of Protein–Small Molecule Complex
Authors: Sisavath Nicolas , Rukundo Jean‐Luc , Le Blanc J. C. Yves , Galievsky Victor A. , Bao Jiayin , Kochmann Sven , Stasheuski Alexander S. , Krylov Sergey N. 
Published: March 2019

AbstractCurrent practical methods for finding the equilibrium dissociation constant, Kd, of protein–small molecule complexes have inherent sources of inaccuracy. Introduced here is “accurate constant via transient incomplete separation” (ACTIS), which appears to be free of inherent sources of inaccuracy. Conceptually, a short plug of the pre‐equilibrated protein–small molecule mixture is pressure‐propagated in a capillary, causing fast transient incomplete separation of the complex from the unbound small molecule. A superposition of signals from these two components is measured near the capillary exit and used to calculate a fraction of unbound small molecule, which, in turn, is used to calculate Kd. Herein the validity of ACTIS is proven theoretically, its accuracy is verified by computer simulation, and its practical use is demonstrated. ACTIS has the potential to become a reference‐standard method for determining Kd values of protein–small molecule complexes.
The Furan Shuffling Hypothesis: A Biogenetic Proposal for Eremophilane Sesquiterpenoids
Authors: Lardon Nicolas , Liffert Raphael , Linden Anthony , Gademann Karl 
Published: March 2019

AbstractBased on the structural similarities of the recently isolated eremophilane‐type sesquiterpenoids microsphaeropsisin B and C and the iso‐eremophilane periconianone C, a revised biogenetic hypothesis for C8–C11‐connected iso‐eremophilanes is presented and corroborated by strong experimental evidence. The first enantioselective total syntheses of microsphaeropsisin B and C were achieved starting from a known intermediate, whose synthesis was elaborated previously in the total synthesis of periconianone A, and in a total of 15 steps starting from γ‐hydroxy carvone. Mild reaction conditions for the subsequent α‐ketol rearrangement not only resulted in the herein proposed conversion of microsphaeropsisin B into periconianone C, but also in the conversion of microsphaeropsisin C into 4‐epi‐periconianone C.
A switched dynamic model for pointing tasks with a computer mouse
Authors: Aranovskiy Stanislav , Ushirobira Rosane , Efimov Denis , Casiez Géry 
Published: March 2019

AbstractIn this paper, we study one of the most fundamental tasks in human‐computer interaction, the pointing task. It can be described simply as reaching a target with a cursor starting from an initial position (e.g. executing a movement using a computer mouse to select an icon). In this paper, a switched dynamic model is proposed to handle cursor movements in indirect pointing tasks. The model contains a ballistic movement phase governed by a nonlinear model in Lurie form and a corrective movement phase described by a linear visual‐feedback system. The stability of the model is first established and the derived model is then validated with experimental data acquired in a pointing task with a mouse. It is established that the measured data of pointing movements of different types can be fitted within the proposed model. Numerical comparison against pointing models available in the literature is also provided.
FPGA implementation of extended Kalman filter for SOC estimation of lithium‐ion battery in electric vehicle
Authors: Ma Yan , Duan Peng , He Pengcai , Zhang Fan , Chen Hong 
Published: March 2019

AbstractA FPGA implementation for a model‐based state of charge (SOC) estimation is described in this paper. A Thevenin equivalent circuit model is designed for SOC estimation. The extended Kalman filter (EKF) is designed to complete the SOC estimation, and the error is within 1 % . The FPGA is chosen to achieve realtime SOC estimation. A fast matrix method is proposed to improve the calculation speed of the EKF in FPGA because the EKF algorithm requires many matrix operations. In addition, the embedded system based on the FPGA with a system on a programmable chip (SOPC) technique is built using the Qsys platform in Quartus II. Based on the embedded system, an online testing platform is established to monitor the terminal voltage and load current of the experimental battery in real time; experimental results show that the online SOC estimation is successful. The measurement results show that the FPGA embedded scheme of the EKF allows for successful implementation of the SOC estimation with accuracy and speed. The fast matrix method requires 0.00007 s to implement the SOC estimation and is four times faster than the conventional matrix method.
Maintenance optimization for second‐hand products following periodic imperfect preventive maintenance warranty period
Authors: Lim Jae‐Hak , Kim Dae‐Kyung , Park Dong Ho 
Published: March 2019

AbstractThe maintenance policy for a product's life cycle differs for second‐hand and new products. Although several maintenance policies for second‐hand products exist in the literature, they are rarely investigated with reference to periodic inspection and preventive maintenance action during the warranty period. In this research, we study an optimal post‐warranty maintenance policy for a second‐hand product, which was purchased at age x with a fixed‐length warranty period. During the warranty period, the product is periodically inspected and maintained preventively at a prorated cost borne by the user, while any product failure is only minimally repaired by the dealer. After the warranty expires, the product is self‐maintained by the user for a fixed‐length maintenance period and the costs incurred during this time are fully borne by the user. At the end of the maintenance period, the product is replaced with a product of the user's choice. This study is focused on the determination of an optimal length for the maintenance period after the warranty expiration. As a criterion for the optimality, we adopt the long‐run mean cost during the second‐hand product's life cycle from the user's perspective. Finally, our results are analyzed numerically for sensitive analysis of several relevant factors, assuming that the failure distribution follows a Weibull distribution.
An integration of algal biofuel production planning, scheduling, and order‐based inventory distribution control systems
Authors: Nugroho Yohanes K. , Zhu Liandong 
Published: March 2019

AbstractThis paper studies an integration model of production planning, scheduling, and order‐ based inventory distribution control systems. It models supply‐chain and production dynamics and employs process control to supply‐chain operations and the production process and scheduling. It embeds generalized disjunctive programming (GDP) and branch‐and‐reduce algorithms to obtain an optimal solution. Generalized disjunctive programming reformulates the original mixed integer nonlinear programming (MINLP) model and solves the new model with a lower number of integer variables. Afterwards, the branch and reduce algorithm, which is strengthened with outer approximation (OA) cutting planes, is able to find optimal solutions with permissible optimality gaps. The results show that supply network size does not affect forecast accuracy; there is a forecast error rate of 12.9%. Similar figures are also shown for the inventory level, which is expected to be 32% of demand level, production changeover time at 0.58 days, inventory ramp‐up period to meet the expected raw materials and final products inventory at 0.02 and 0.04 days respectively, and extra delivery time to meet a certain level of demand change at 1.1 days. The results show that the model is capable of controlling demand forecast, inventory level, and delivery quantity, so that the supply chain can avoid stock out at a minimal level of inventory, regardless of the size of supply‐chain networks. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd.
Bewertung von Bestandsbauwerken mit gemessenen Daten
Authors: Küttenbaum Stefan , Maack Stefan , Braml Thomas , Taffe Alexander , Haslbeck Matthias 
Published: March 2019

AbstractGegenüber dem Neubauentwurf können bei der Bewertung von bestehender Bausubstanz Sicherheitsreserven genutzt werden, da Unsicherheiten, die beim Bau auftreten können, besser bekannt oder nicht mehr vorhanden sind. Können solche Unsicherheiten genauer bewertet werden, z. B. durch die Feststellung der genauen Lage der Spannglieder bei einer Spannbetonbrücke, so können Sicherheitsbeiwerte reduziert werden, ohne dass es dabei zu Auswirkungen auf das normativ festgelegte Zuverlässigkeitsniveau kommt. Mittlerweile sind Prüfmethoden an Bauwerken wirtschaftlich einsetzbar und auch so leistungsfähig, dass die für die Tragfähigkeit und Gebrauchstauglichkeit wesentlichen Parameter identifiziert werden können. Ein wesentlicher Punkt ist hierbei die Integration derartiger Messergebnisse in Rechenmodelle, die für die Nachrechnung des Bauwerks verwendet werden. Dies können sowohl semiprobabilistische Rechenmodelle als auch vollprobabilistische Modelle sein. Bei semiprobabilistischen Methoden können aus den Messergebnissen Teilsicherheitsbeiwerte berechnet und abgeleitet werden, die dann in den bekannten Nachweisformaten gemäß den Eurocodes und der Nachrechnungsrichtlinie berücksichtigt werden. Bei vollprobabilistischen Nachweisen können die Messdaten in Form von Verteilungsdichtefunktionen mit gemessenen Variationskoeffizienten direkt in das Rechenmodell eingehen. In einer dreiteiligen Aufsatzreihe werden die Messverfahren und die Nutzung der Ergebnisse bei der Nachrechnung vorgestellt. Der vorliegende Teil 1 zeigt die Möglichkeiten des Einsatzes von zerstörungsfreien Prüfverfahren und bewertet deren Leistungsfähigkeit. Messen heißt wissen. Dieses Wissen spiegelt die Realität wider und soll den Tragwerksplaner bei der Entscheidungsfindung über die Sicherheit und Zuverlässigkeit von Bestandsbauwerken unterstützen.
miR‐155‐5p and miR‐760 mediate radiation therapy suppressed malignancy of non‐small cell lung cancer cells
Authors: Zhu Lin , Xue Feng , Cui Ying , Liu Shanshan , Li Gen , Li Jian , Guan Bixi , Zeng Hai , Bian Weixin , Yang Chuan , Zhao Chunbo 
Published: March 2019

AbstractMicroRNAs (miRNAs) play important roles in tumorigenesis of various cancers. Recent study suggested that miRNAs are involved in the therapeutic functions of radiation during cancer treatment. We found that radiation can decrease the migration and invasion of non‐small cell lung cancer (NSCLC) cells. Mechanistically, radiation can significantly increase the expression of miR‐155‐5p and miR‐760 in NSCLC cells. Knockdown of miR‐155‐5p and miR‐760 can attenuate radiation suppressed proliferation of NSCLC cells. Among the various targets of miR‐155‐5p, radiation can decrease the expression of HIF‐1α. Similarly, radiation can also suppress the expression of IL‐6 via a miR‐760 dependent pathway. Gain and loss of function studies confirmed that both HIF‐1α and IL‐6 were involved in the radiation suppressed proliferation of NSCLC cells. Collectively, our data showed that radiation can regulate the expression of miR‐155‐5p and miR‐760 to suppress the malignancy of NSCLC cells. © 2019 BioFactors, 45(3):393–400, 2019
Study of diffusion‐weighted magnetic resonance imaging in the evaluation of the response to AAV2‐VEGF‐Trap neoadjuvant treatment in a triple‐negative breast cancer animal model
Authors: Li Jianhua , Zhu Pengjin , Wang Lei , Yang Li , Zou Liqun , Gao Fabao 
Published: March 2019

AbstractObjectiveEvaluation of the efficacy of adeno‐associated virus 2 mediated gene transfer of vascular endothelial growth factor Trap (AAV2‐VEGF‐Trap) alone or combination with paclitaxel in a mouse model of triple‐negative breast cancer (TNBC) using diffusion‐weighted magnetic resonance imaging (DW‐MRI) and in vivo fluorescence imaging.Materials and MethodsXenografted TNBC tumors were established by subcutaneous injection of MDA‐MB‐231 cells into nude mice. Tumors were treated with AAV2‐VEGF‐Trap, paclitaxel, AAV2‐VEGF‐Trap combined with paclitaxel and control. A 7.0‐Tesla magnetic resonance (MR) was used to obtain the apparent diffusion coefficient (ADC) values and ΔADC values. In vivo fluorescence imaging coupled with the optical imaging probe AngioSense680 EX was acquired to obtain average luminous intensity values. Immunohistochemical staining of tumor Ki‐67 and vascular endothelial cell marker antigen (CD31) were used to evaluate the effects on tumor proliferation and angiogenesis.ResultsThe combination of AAV2‐VEGF‐Trap with paclitaxel exhibited greater tumor growth inhibition compared with the other groups. The ADC values in the paclitaxel group and the AAV2‐VEGF‐Trap in combination with paclitaxel group were significant greater compared with the control group, and the ΔADC values of all treatment groups were significantly increased compared with the control group on the 14th day after administration. Decreased microvessel density and luminous intensity in the treatment groups that contain AAV2‐VEGF‐Trap were observed. Reduced proliferation activity was noted in groups that contained paclitaxel.ConclusionAAV2‐VEGF‐Trap inhibits TNBC growth though inhibiting tumor neovascularization with a single intravenous injection, and AAV2‐VEGF‐Trap exhibits a synergistic effect when used in combination with paclitaxel for TNBC neoadjuvant therapy. In vivo fluorescence imaging can detect the anti‐angiogenesis effect of AAV2‐VEGF‐Trap early and noninvasively. DW‐MRI can longitudinally monitor the neoadjuvant efficacy of TNBC.
Fungicidal Properties of Some Novel Trifluoromethylphenyl Amides
Authors: Tsikolia Maia , Bernier Ulrich R. , Wedge David E. , Tabanca Nurhayat , Abboud Khalil A. , Linthicum Kenneth J. 
Published: March 2019

AbstractTrifluoromethylphenyl amides (TFMPAs) were designed and synthesized as potential pesticides. Thirty‐three structures were evaluated for fungicidal activity against three Colletotrichum species using direct bioautography assays. Active compounds were subsequently tested against C. fragariae, C. gloeosporioides, C. acutatum, Phomopsis obscurans, P. viticola, Botrytis cinerea and Fusarium oxysporum. The study identified 2‐chloro‐N‐[2,6‐dichloro‐4‐(trifluoromethyl)phenyl]acetamide (7a) as showing the strongest antifungal activity, and the broadest activity spectrum in this set against Colletotrichum acutatum (at 48 and 72 h) and Phomopsis viticola (at 144 h). The presence of triethylamine in its complex with N‐[2,6‐dichloro‐4‐(trifluoromethyl)phenyl]‐2,2,3,3,3‐pentafluoropropanamide (7b′) played an important role in the bioactivity, and depending on the concentration or fungal species it showed higher or lower activity than the parent amide. X‐Ray crystallography has shown that the complex (7b′) is an ion pair, (C10H2Cl2F8NO)− (C6H16N)+, where a proton is transferred from the amide nitrogen to the triethylamine nitrogen and then connected by hydrogen bonding to the acyl oxygen (N−H 0.893 Å; H⋅⋅⋅O 1.850 Å; N⋅⋅⋅O 2.711 Å; N−H⋅⋅⋅O 161.2(13)°). Although none of these compounds were better than standards, this work revealed some potential lead structures for further development of active novel compounds.