bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association between blood vitamin E and blood pressure in an adult population with and without diabetes mellitus
Authors: Wan Rong , Su Yuhao , Zhu Meilan , Huang Ying 
Published: November 2024

AimsPrevious studies on the association between vitamin E and blood pressure (BP) levels are controversial. Our study aimed to evaluate the association between blood vitamin E (alpha-tocopherol and gamma-tocopherol) levels and systolic and diastolic BP in an adult population with diabetes and without diabetes.MethodsOur study data were obtained from a biomarker project of the Midlife in the United States (MIDUS) study. A total of 1068 subjects were included, and the associations between alpha-tocopherol and gamma-tocopherol levels and systolic and diastolic BP were further analyzed by smooth curve and multivariate linear regression analyses.ResultsOur smooth curve analysis showed an almost linear correlation between blood vitamin E (alpha-tocopherol and gamma-tocopherol) levels and systolic and diastolic BP. Furthermore, we found that blood gamma-tocopherol levels were positively and independently associated with systolic BP (B=0.427, 95% CI 0.067-0.787, P=0.020) and diastolic BP (B=0.289, 95% CI 0.072-0.507, P=0.009) when the data were adjusted for age, gender, body mass index (BMI), ever smoked cigarettes regularly, number of years of consuming alcohol and regular exercise or activity for 20 minutes or more at least 3 times/week. Consistently, blood alpha-tocopherol levels were also positively associated with systolic BP (B=0.150, 95% CI 0.064-0.235, P=0.001) and diastolic BP (B=0.056, 95% CI 0.004-0.107, P=0.035) after these variables were adjusted. However, these significant relationships exist only in subjects without diabetes, but not in subjects with diabetes.ConclusionsWe observed for the first time that blood vitamin E (alpha-tocopherol and gamma-tocopherol) levels were positively associated with systolic and diastolic BP in subjects without diabetes.
Ocean literacy research community: co-identifying gaps and priorities to advance the UN Ocean Decade
Authors: McRuer Jen , McKinley Emma , Glithero Diz L. , Paiz-Domingo Martha 
Published: November 2024

IntroductionThe overarching goal of the UN Ocean Decade is to “change humanity's relationship with the ocean.” While this may be a challenge, it is, at the same time, a once in a generation opportunity. How can 8 billion people, including those who don't live near coastal areas, be inspired to value and care for the ocean? This is the essence of ocean literacy, and the driver of ocean literacy research (OLR).MethodsIn 2021, we began a research initiative to co-create a global OLR agenda by the developing OLR community, to better understand existing research themes, gaps, future priorities, actions, and impacts of ocean literacy initiatives. To deliver this, a series of virtual workshops – with the first taking place as part of the UN Ocean Decade Laboratories – was complemented by a participatory methodology using digital survey and mapping tools for crowdsourced collaboration.Results and discussionThrough this process, four initial OLR priorities were identified, including measuring ocean literacy, the role of ocean literacy as a policy mechanism, and alignment of OLR with climate change and the blue economy agendas. Finally, a working definition of OLR was developed to further guide OLR priorities for the UN Ocean Decade and beyond.
Research on gut microbiota characteristics of PBC patients at different ALBI grades based on machine learning
Authors: Shi Han , Wang Qi , Xu Bin , Liu Yanmin , Zhao Juan , Yang Xue , Huang Chunyang , Jin Ronghua 
Published: November 2024

BackgroundThe Albumin-Bilirubin (ALBI) score and grade are widely used to stratify patients with primary biliary cholangitis (PBC) into different disease statuses and risk levels. Recent studies have increasingly highlighted the role of gut microbiota in autoimmune liver diseases. This study aimed to investigate the differences in gut microbiota among PBC patients with varying ALBI grades.MethodsClinical data and stool samples were collected from outpatient and inpatient PBC patients between 2019 and 2022. Gut microbiota profiles were obtained using 16S rDNA sequencing of stool samples. We analyzed alpha diversity, beta diversity, LEfSe analysis and pathway function prediction. Additionally, various machine learning methods—including random forest (RF), lasso, gradient boosting machine (GBM) and support vector machine (SVM)—were employed to identify key features and to build and validate predictive models using bootstrap techniques.ResultsClinical characteristics of ALBI grade 1 patients were comparatively better than those of ALBI grade 2 and 3 patients, including multiple laboratory indices. Gut microbiota analysis revealed that species richness and balance were higher in ALBI grade 1 patients. Both the comparison of the most abundant genera and the linear discriminant analysis (LDA) in LEfSe demonstrated that Lachnospira had a higher abundance and better discriminative ability in ALBI grade 1. Pathway function prediction indicated that sulfur metabolism was upregulated in higher ALBI grades. Furthermore, RF identified 10 specific genera, which were then used to build and validate models for discriminating PBC patients according to their ALBI grades. All three models, developed using different machine learning methods, demonstrated good discrimination ability (mean AUC 0.75–0.80).ConclusionThis study highlights significant differences in gut microbiota profiles among PBC patients with different ALBI grades. The increased abundance of Lachnospira and upregulation of sulfur metabolism pathways are notable in patients with lower ALBI grades. The machine learning models developed based on gut microbiota features offer promising tools for discriminating between PBC patients with varying disease severities, which could enhance the precision of treatment strategies.
Influence of altitudes and development stages on the chemical composition and antioxidant capacity of Andean blackberries (Rubus glaucus Benth)
Authors: Guevara-Terán Mabel , Tejera Eduardo , Vásquez-Castillo Wilson , Santos-Buelga Celestino , González-Paramás Ana M. , Alvarez-Suarez José M. 
Published: November 2024

BackgroundThe Andean blackberry (Rubus glaucus Benth), locally known as “Mora de Castilla,” is a high-altitude fruit rich in phytochemicals, mainly anthocyanins, with potent antioxidant properties. Although its chemical composition has been studied, the influence of altitude on its phytochemical profile and antioxidant capacity is still unclear. This research aimed to investigate the effects of development stages and altitude on the phytochemical profile and antioxidant activity of this fruit.MethodsAndean blackberry fruits were collected at different altitudes and development stages in the central Andean region of Ecuador. An hydroalcoholic extraction was used to quantify the compounds and determine the total antioxidant activity, while solid phase extraction (SPE) was performed to separate anthocyanins from other compounds. Ultra-high-performance liquid chromatography coupled to a triple quadrupole mass spectrometer (HPLC-DAD/ESI-MSn) was used to identify anthocyanin and non-anthocyanin phenols, while total antioxidant capacity, total polyphenols, total flavonoid content, and total anthocyanin content were quantified spectrophotometrically.ResultsFlavonols and anthocyanins constitute the majority of the flavonoids identified in the Andean blackberry extracts, both in terms of peak areas and number of identified compounds, followed by ellagic and gallic acid derivatives, as well as phenolic acids, mainly hydroxycinnamic acids. Quercetin was identified as the predominant flavonol in unripe berries, where anthocyanins were not significantly present. On the contrary, in ripe berries, cyanidin and its glycosides stood out as the main anthocyanins and predominant compounds. We observed that in the early stages of ripening, the total polyphenol content predominates in the berries and is mainly responsible for their antioxidant capacity. However, as the fruit ripens, the total anthocyanin content increases, becoming the most prominent bioactive compounds in fully ripe berries.ConclusionThe results suggest that higher altitude environmental conditions may improve the composition, concentration of phenolic compounds, and antioxidant activity of Andean blackberries. Overall, our findings highlight the high functional value of this fruit, supporting its health-protective effects when consumed regularly, either as fresh fruit or in nutraceutical form.
Investigation on improving immunologic reconstitution insufficiency using DiwuYanggan capsules in AIDS patients
Authors: Ke Jing Wen , Chen Yao , Lei En Ze , Xiao Ming Zhong , Ni Wei , Huang Fang , Li Han Min , Jiang Hong Lin , Ruan Lian Guo , Liu Jian Zhong 
Published: November 2024

BackgroundThis study aimed to explore the mechanism of action of DiWuYangGan (DWYG) capsule in improving Immunological non-responder (INR) by analyzing the active ingredients of DWYG.MethodsThe study employed a randomized, controlled, double-blind, single-simulation method. Patients were randomly divided into control and trial groups and treated with the primal highly effective antiretroviral therapy. To demonstrate the effect of DWYG on INR, patients in the control group were administered simulated DWYG, whereas patients in the trial group were administered DWYG capsules (ChiCTR1900024673). The chemical composition of DWYG was analyzed using ultra-performance liquid chromatography-high-resolution mass spectrometry. Potential targets of DWYG in the treatment of INR were identified and predicted using network pharmacology and molecular docking. The molecular mechanisms underlying the effects of DWYG were validated using a peripheral blood monocyte model.ResultsThe CD4:CD8 ratio in the trial group was significantly higher than that in the control group (p &lt; 0.01). A total of 210 DWYG compounds were identified and network pharmacology revealed 182 potential therapeutic targets for DWYG and INR. The results of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that the toll-like receptor signaling pathway is one of the key pathways. This study demonstrated that DWYG reduced the expression level of TLR4 and the levels of IL-2, IL-10, and TNF-α, which are important cytokines involved in the immune response.ConclusionThe efficacy of DWYG in the treatment of INR confirmed the potential practical components of DWYG. Moreover, the results of network pharmacology and experimental validation showed that DWYG could restore the immune function of acquired immune deficiency syndrome patients by inhibiting the expression of TLR4 and related signaling pathways and the overactivation of immune function.Clinical Trial Registration:https://www.chictr.org.cn/index.html, identifier ChiCTR1900024673.
Formulation-dependent differences in paclitaxel distribution to anatomical sites relevant to chemotherapy-induced peripheral neuropathy
Authors: Girdenytė Milda , Hu Yang , Ginosyan Aghavni , Hammarlund-Udenaes Margareta , Loryan Irena 
Published: November 2024

IntroductionChemotherapy-induced peripheral neuropathy (CIPN) is a dose-limiting adverse event observed in patients receiving paclitaxel, associated with initial pathological changes in the peripheral nervous system, i.e., distal nerves and dorsal root ganglia (DRG). The prevalence of CIPN in patients receiving paclitaxel formulated i) in polyethylated castor oil with ethanol (CreEL-PTX), ii) as albumin-bound (nab-PTX), and iii) in XR17 micelles (micellar-PTX), is unexpectedly varying. We hypothesize that the discrepancy in CIPN prevalence could be governed by differences in the extent of paclitaxel distribution across blood-to-tissue barriers at the CIPN-sites, caused by the specific formulation.MethodsThe recently developed Combinatory Mapping Approach for CIPN was used to determine the unbound tissue-to-plasma concentration ratio Kp,uu,tissue, after a 4-h infusion of 4 mg/kg CreEL-PTX, 4 mg/kg nab-PTX or 1 mg/kg micellar-PTX in male and female Sprague Dawley rats. Kp,uu,tissue was determined in conventional (DRG, sciatic nerve) and non-conventional (brain, spinal cord, skeletal muscle) CIPN-sites.ResultsBased on our data, the Cremophor-free paclitaxel formulations were associated with a higher distribution of paclitaxel to CIPN-sites than CreEL-PTX, e.g., Kp,uu,DRG of 0.70 and 0.60 for nab-PTX and micellar-PTX, respectively, in comparison to 0.27 for CreEL-PTX (p &lt; 0.01). In addition, the fraction of unbound paclitaxel in plasma was on average 1.6-fold higher in nab- and micellar PTX arms and equal to 0.061 and 0.065, respectively, compared to 0.039 for the CreEL-PTX treatment arm (p &lt; 0.0001).DiscussionIn the case of similar unbound paclitaxel concentration in the plasma of patients and assumed species-independent extent of paclitaxel transport across the barriers, nab- and micellar-PTX formulations can lead to higher paclitaxel exposure at CIPN-sites in comparison to CreEL-PTX.
Autism, intelligence, language, and adaptive behavior, disentangling a complex relationship
Authors: Failla Chiara , Scarcella Ileana , Vetrano Noemi , Previti Serena , Mangano Rita Mangano , Tartarisco Gennaro , Vagni David , Pioggia Giovanni , Marino Flavia 
Published: November 2024

IntroductionAutism Spectrum Disorder (ASD) is a complex neurodevelopmental disorder characterized by a range of intellectual and language abilities. Its heterogeneity is acknowledged in modern diagnostics, complicating research and necessitating precision medicine and a multidimensional approach for individualized treatment and accurate assessment. Intellectual and language functioning influence adaptive skills and symptomatology. Thus, assessing adaptive functioning in a multidimensional and multi-informant manner is crucial, highlighting the importance of comprehensive evaluations. This study explores the interplay between autistic traits, demographic variables, IQ, adaptive functioning, and the applicability of ICD-11 classifications.MethodsWe analyzed data from the initial global evaluation of 60 diverse autistic children (aged 35 to 120 months; IQ range 16 to 118). Parent-reports using the Vineland Adaptive Behavior Scale (VABS-II) were compared with standardized assessments from the PsychoEducational Profile (PEP-3). Children’s intellectual levels were assessed using Griffiths Scales of Child Development (Griffiths III) and the Autism Diagnostic Observation Schedule (ADOS-2) was used for autistic traits. They were further classified according to the ICD-11 diagnostic system, functional language, and intellectual functioning levels. Correlations among variables, group comparisons, and multivariate analyses were performed.ResultsThe analysis indicates a linear effect of IQ on all adaptive scales and the impact of autistic traits on directly measured adaptive functioning. A factorial effect was observed due to changes concerning specific age, intellectual, and linguistic levels, which do not completely align with ICD-11 categorization. Additionally, a negative correlation between intelligence and measured autistic traits was found. Parental age, education level, and age at childbirth were also found to affect various adaptive scales.DiscussionThe study questions the ICD-11’s proposed distinctions in IQ and language functioning for ASD, advocating for more refined categorization and developmental considerations. It underscores the intricate relationship between autistic traits, IQ, and communication skills, casting doubt on the precision of diagnostic tools across the spectrum. Parental reports and direct assessments are essential for comprehensive evaluation, with parental education and age influencing children’s behaviors and skills. The study calls for a nuanced approach to ASD assessment, integrating various metrics and sources of information for a detailed phenotyping necessary for personalized interventions and biological research.
Pros and cons of digital access to healthcare services and healthcare utilization: a quasi-experiment
Authors: Antinyan Armenak , Bellio Stefania , Bertoni Marco , Corazzini Luca , Longone Michela , Narne Elena 
Published: November 2024

ObjectivesWe study the introduction of a web portal allowing women to manage their appointments in the public cervical cancer screening program of an Italian Local Health Unit (LHU). We investigate how this intervention affects program participation and the ability of the LHU to collect information on women's screening outside the program.MethodsWe use administrative data from the LHU and a difference-in-differences design to compare outcomes of women in the treated group (n=870), who could reschedule appointments either through phone calls or through the portal, and the control group (n=768), who could only reschedule by phone.ResultsThe portal reduces the number of appointments rescheduled by phone (b = −0.176, 95% CI = −0.228 to −0.114, p &lt; 0.001). It also makes women more likely to report both screenings outside the program (b = 0.049, 95% CI = 0.006–0.912, p &lt; 0.05) and the various reasons for canceling their appointments. However, the portal also decreases the probability of screening (b = −0.156, 95% CI = −0.216–0.096, p &lt; 0.001). Two-thirds of this reduction is due to increased unjustified no-shows (b = 0.099, 95% CI = 0.043–0.155, p &lt; 0.001).ConclusionsDigital innovations increase efficiency of screening programs. However, they may discourage screening, by triggering behavioral biases such as limited attention and procrastination. Pairing portals with frequent reminders may help to boost participation.
Experimental analysis of glass failure criteria under different thermal conditions
Authors: Mishra Raj Kumar , Sharma Pawan Kumar , Kumar Ravi 
Published: November 2024

The initiation of the first crack mainly determines the criteria for glass failure. However, the extended consequences of cracking, with the formation of multiple cracks merging, result in fallout conditions under varying thermal loads that become more critical. Point-supported glass arrangements are globally used in high-rise and energy-efficient buildings for architectural ingenuity aimed at a low budget. However, the formation of cracks due to thermal load resulting in glass breakage could infuriate the overall fire dynamics of the enclosed area. Therefore, experimental study and prediction of glass failure become very crucial. The present experimental study focuses on finding the most critical parameter that may quantify the cause of glass failure and further breakage in fallout conditions under varying thermal loads. 45 experiments were carried out on float glass of 300 × 300 mm2 with 4 mm, 6 mm, 8 mm, 10 mm, and 12 mm with continuous fuel supply arrangements. Critical parameters analysed were the time of crack initiation, glass temperature at the time of cracking, maximum temperature difference at glass failure, and thermal strain caused by the temperature difference on the glass surface. The range of minimum and maximum temperature difference recorded on the glass surface for the present study was between 30–35°C and 55–60°C at the breakage time for all the experiments. The Maximum temperature difference measured was 56.99°C on the 12 mm glass surface, and the corresponding maximum thermal strain found was 482 × 10−6 mm/mm. The maximum heat release rate was found to be approximately 200 kW. Maximum heat flux was found in the range of 10.33 kW/m2 to 21.14 kW/m2. A correlation was also developed using the least square method for all the thicknesses, which is well correlated with the glass thickness per unit length.
A multimodal in vitro approach to assess the safety of oral care products using 2D and 3D cellular models
Authors: Leano S. Marceli , De Souza Wanderson , De Vecchi Rodrigo , Lopes Amanda , Deliberador Tatiana , Granjeiro Jose M. 
Published: November 2024

IntroductionPeriodontitis, affecting approximately 3.9 billion individuals globally, significantly impacts quality of life and has raised interest in its potential systemic effects. Sodium perborate, a common component in oral care products for biofilm control, is widely used, though concerns about its safety persist. This study aimed to evaluate the in vitro toxicity of six commercial oral care products and varying concentrations of sodium perborate, utilizing human gingival fibroblasts (HGF) and keratinocytes (HaCat) as cell models.MethodsExperiments were performed in both 2D monolayer and 3D cultures using MTT and electrical impedance assays, adhering to the manufacturer’s recommended exposure time of 30–60 s for product testing. For the reconstructed epidermis model, a prolonged exposure time of 42 min was applied, following the Organization for Economic Cooperation and Development (OECD) Test Guideline 439.ResultsResults indicated that all products and sodium perborate at 1 mg/mL were cytotoxic in monolayer cultures. However, at concentrations relevant to commercial formulations (0.06 mg/mL sodium perborate), no significant toxicity was observed. In contrast, the 3D culture models, including spheroids and reconstructed epidermis, exhibited minimal to no cytotoxic effects for the commercial products, with sodium perborate showing no significant toxicity below 0.1 mg/mL. The reconstructed epidermis model, used as surrogate for oral mucosa, further confirmed that the products were non-irritating, in compliance with OECD TG 439 standards.DiscussionThis study highlights the importance of considering exposure time, dosage, and cellular model when assessing the safety of oral care products. While 2D models are useful for preliminary screenings, 3D models provide a more physiologically relevant assessment, emphasizing the need for robust testing protocols to ensure product safety.
Natural Bactericidal Effects of Psoralea glandulosa Essential Oil for the Control of Bacterial Canker and Speck in Tomato
Authors: Montenegro Iván , Valenzuela Ormeño Miryam , Seeger Michael , Besoain Ximena , Godoy Patricio , Werner Enrique , Caro Nelson , Olguín Yusser , Valenzuela Manuel , Silva Valentina , Madrid Alejandro 
Published: November 2024

Bacterial canker and bacterial speck are diseases affecting tomato caused by Clavibacter michiganensis subsp. michiganensis and Pseudomonas syringae pv. tomato, respectively. These diseases are considered a serious threat with a strong impact on tomato production and marketing worldwide, especially because of their reduced sensitivity to traditional controls. This work reports the chemical composition of the essential oil (EO) of Psoralea glandulosa and investigates its in vitro antimicrobial activity, along with its main compound, against three strains of Clavibacter michiganensis subsp. michiganensis (CmVC533, CmVLC78, and CmVQ59) and one strain of Pseudomonas syringae pv. tomato (Pst). The results indicate that both the EO and bakuchiol have significant antibacterial capacity, especially the EO, which reaches a minimum inhibitory concentration (MIC) between 4–16 µg/mL and 128 µg/mL, and a minimum bactericidal concentration (MBC) between 8–16 µg/mL and 128 µg/mL for the strains of C. michiganensis subsp. michiganensis and P. syringae pv. tomato, respectively. The EO and bakuchiol also had an inhibitory effect when applied directly onto plates seeded with C. michiganensis subsp. michiganensis. Overall, the results from this study should be verified in the near future by in vivo studies.