bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircRBMS3 promotes gastric cancer tumorigenesis by regulating miR‐153–SNAI1 axis
Authors: Li Guangyan , Xue Minghui , Yang Fang , Jin Yuhong , Fan Yingying , Li Wei 
Published: August 2018

AbstractGastric cancer (GC) is one of the leading causes of cancer‐related death worldwide. Mounting evidence showed that circular RNAs (circRNAs) play critical roles in human malignancy. However, the knowledge about circRNAs in GC is still unclear. In the current study, high throughput microarray assay showed that circRBMS3 was upregulated in GC tissues, which was further confirmed by quantitative reverse transcription polymerase chain reaction. Correlation analysis revealed that high circRBMS3 expression was associated with advanced TNM stage, depth of invasion, and lymph‐node metastasis. Kaplan–Meier analysis indicated that GC patients with high circRBMS3 expression have a poor overall survival (OS). Function assays showed that circRBMS3 silencing reduced GC cells proliferation and invasion in vitro, and inhibited the tumor growth in vivo. Mechanistically, we found that miR‐153 could act as a target of circRBMS3. Subsequently, we showed that circRBMS3 promoted snail family zinc finger 1 (SNAI1) expression via inhibiting miR‐153 in GC cells. Collectively, these results suggested that circRBMS3 promoted GC cells proliferation and invasion via regulating miR‐153/SNAI1 axis.
Identification of a five‐gene signature with prognostic value in colorectal cancer
Authors: Sun Guangwei , Li Yalun , Peng Yangjie , Lu Dapeng , Zhang Fuqiang , Cui Xueyang , Zhang Qingyue , Li Zhuang 
Published: August 2018

AbstractColorectal cancer (CRC) ranks as one of the most commonly diagnosed malignancies worldwide. Although mortality rates have been decreasing, the prognosis of CRC patients is still highly dependent on the individual. Therefore, identifying and understanding novel biomarkers for CRC prognosis remains crucial. The gene expression profiles of five‐gene expression omnibus (GEO) data sets of CRC were first downloaded. A total of 352 consistent differentially expressed genes (DEGs) were identified for CRC and paired with normal tissues. Functional analysis including gene ontology and Kyoto encyclopedia of genes and genomes pathway enrichment revealed that these DEGs were related to metabolic pathways, tight junctions, and the cell cycle. Ten hub DEGs were identified based on the search tool for the retrieval of interacting genes database and protein–protein interaction networks. By using univariate Cox proportional hazard regression analysis, we found 11 survival‐related genes among these DEGs. We finally established a five‐gene signature (kinesin family member 15, N‐acetyltransferase 2, glutathione peroxidase 3, secretogranin II, and chloride channel accessory 1) with prognostic value in CRC by step multivariate Cox regression analysis. Based on this risk scoring system, patients in the high‐risk group had significantly poorer survival results compared with those in the low‐risk group (log‐rank test, p < 0.0001). Finally, we validated our gene signature scoring system in two independent GEO cohorts (GSE17536 and GSE33113). We found all five of the signature genes to be DEGs in The Cancer Genome Atlas database. In conclusion, our findings suggest that our five DEG‐based signature can provide a novel biomarker with useful applications in CRC prognosis.
Decrease of MiR‐31 induced by TNF‐α inhibitor activates SATB2/RUNX2 pathway and promotes osteogenic differentiation in ethanol‐induced osteonecrosis
Authors: Yu Lingjia , Xu Yisheng , Qu Hao , Yu Yifeng , Li Wenjing , Zhao Yu , Qiu Guixing 
Published: August 2018

PurposeSuppressed osteogenic differentiation is considered a main cause of ethanol‐induced osteonecrosis. Tumor necrosis factor α (TNF‐α) and miR‐31 have been reported to be involved in the osteogenic induction. This study aimed to explore a possible molecular mechanism regulating osteogenic differentiation in ethanol‐induced osteonecrosis bone marrow stromal stem cells (BMSCs).MethodsAlizarin red staining was used to examine the level of mineralization in osteogenic differentiation process. Alkaline phosphatase assay was applied to the validation of ALP level which was essential to bone mineralization. The level of osteogenesis markers was determined by western blot assay, whereas the fluctuations of messenger RNA levels were tested by quantitative real‐time polymerase chain reaction. Microarray analysis was conducted to identify differentially expressed genes, because the possible target relationship was predicted and validated by miRBase and luciferase reporter assay, respectively. Colony forming unit of fibroblast assay was used to observe the proliferation of BMSCs.ResultsBMSCs from patients with ethanol‐induced osteonecrosis exhibited weaker osteogenic differentiation and proliferation abilities. TNF‐α inhibitor added in the osteogenic medium significantly enhanced the osteogenic differentiation ability and BMSCs proliferation ability. TNF‐α by regulating miR‐31 downregulated the expressions of RUNX2 and SATB2, two contributors of osteoblast differentiation, further suppressed osteogenic differentiation. On the contrary, TNF‐α inhibitor could promote osteogenic differentiation in BMSCs from patients with ethanol‐induced osteonecrosis.ConclusionTNF‐α inhibitor could downregulate miR‐31 expressions, which directly promoted SATB2 and RUNX2 expressions and enhanced osteogenic differentiation of BMSCs from patients with ethanol‐induced osteonecrosis.
Recent trends in the occurrence of bleeding gastric and duodenal ulcers under the Japanese evidence‐based clinical practice guideline for peptic ulcer disease
Authors: Nagashima Kazunori , Tominaga Keiichi , Fukushi Koh , Kanamori Akira , Sasai Takako , Hiraishi Hideyuki 
Published: August 2018

BackgroundGastrointestinal hemorrhage occurs frequently. We reviewed the tendency of occurrence of bleeding gastric and duodenal ulcers and their association with antithrombotic therapy before and after the widespread use of Evidence‐Based Clinical Practice Guidelines for Peptic Ulcer 2009 (1st edition), which was published to improve treatment outcomes and prevent peptic ulcers.MethodsThe study enrolled 1105 patients with bleeding gastric and duodenal ulcers treated at our hospital between January 2000 and March 2016. They were divided into the preguideline group (807 patients treated between January 2000 and December 2010) and the postguideline group (298 patients treated between January 2011 and March 2016). The use of medications, severity, the incidence of Helicobacter pylori infection, the presence of any underlying disease, and other factors were compared between the pre‐ and postguideline groups.ResultsThe number of patients receiving antithrombotic therapy was slightly higher in the postguideline group without a significant difference (P = 0.50). The incidence of H. pylori infection was significantly lower in the postguideline group (P < 0.001). The rate of premedication with a proton pump inhibitor (PPI) and the rate of severe ulcers were significantly higher in the postguideline group (P = 0.001 and P < 0.001, respectively). The rebleeding rate showed no significant difference, whereas the recurrence rate was significantly higher in the postguideline group (P = 0.041).ConclusionsThe major cause of hemorrhagic gastroduodenal ulcers seems to be shifting from H. pylori infection to the administration of drugs with gastrointestinal risk. Antithrombotic therapy tends to be associated with severe ulcers but without statistical significance.
Minimal conditioning in Fanconi anemia promotes multi‐lineage marrow engraftment at 10‐fold lower cell doses
Authors: Haworth Kevin G. , Ironside Christina , Ramirez Megan A. , Weitz Sarah , Beard Brian C. , Schwartz Jonathan D. , Adair Jennifer E. , Kiem Hans‐Peter 
Published: August 2018

AbstractBackgroundGene therapy approaches for the treatment of Fanconi anemia (FA) hold promise for patients without a suitably matched donor for an allogeneic bone marrow transplant. However, significant limitations include the collection of sufficient stem cell numbers from patients, the fragility of these cells during ex vivo manipulation, and clinically meaningful engraftment following transplantation. With these challenges in mind, we were interested in determining (i) whether gene‐corrected cells at progressively lower numbers can successfully engraft in FA; (ii) whether low‐dose conditioning facilitates this engraftment; and (iii) whether these cells can be selected for post‐transplant.MethodsUtilizing a well characterized mouse model of FA, we infused donor bone marrow from healthy heterozygote littermates that are unaffected carriers of the FANCA mutation to mimic a gene‐corrected product, after administering low‐dose conditioning. Once baseline engraftment was observed, we administered a second, very‐low selective dose to determine whether gene‐corrected cells could be selected for in vivo.ResultsWe demonstrate that upfront low‐dose conditioning greatly increases successful engraftment of hematopoietic corrected cells in a pre‐clinical animal model of FA. Additionally, without conditioning, cells can still engraft and demonstrate a selective advantage in vivo over time following transplantation, and these corrected cells can be directly selected for in vivo after engraftment.ConclusionsMinimal conditioning prior to bone marrow transplant in Fanconi anemia promotes the multi‐lineage engraftment of 10‐fold fewer cells compared to nonconditioned controls. These data provide important insights into the potential of minimally toxic conditioning protocols for FA gene therapy applications.
Follicular regulatory T cell in atherosclerosis
Authors: Baptista Daniela , Mach François , Brandt Karim J 
Published: August 2018

Abstract
Atherosclerosis is a chronic inflammatory disease involving the infiltration of immune cells, such as monocytes/macrophages, neutrophils, T cells, and B cells, into the inner layer of vessel walls. T and B cell functions in the process of atherogenesis, as well as their mutual regulation, have been investigated but several aspects remain to be clarified. In the present review, we give a brief overview of the functions of follicular regulatory T cell (Tfr) on follicular T (Tfh) and B cell regulation related to atherosclerosis pathogenesis, including their influence on lymphangiogenesis and lipoprotein metabolism. We will also discuss their potential therapeutics properties in the resolution of established atherosclerotic lesions.
Tfr cells are key regulators of atherosclerosis through regulation of Tfh cell population, inducing expansion of atheroprotective Breg cell population and stimulating lymphangiogenesis.
Nitrogen and sulfur co‐doped carbon nanodots toward bovine hemoglobin: A fluorescence quenching mechanism investigation
Authors: Wang Jiajun , Xiang Xueqing , Milcovich Gesmi , Chen Jingru , Chen Chao , Feng Jiuju , Hudson Sarah P. , Weng Xuexiang , Ruan Yongming 
Published: August 2018

AbstractA deep understanding of the molecular interactions of carbon nanodots with biomacromolecules is essential for wider applications of carbon nanodots both in vitro and in vivo. Herein, nitrogen and sulfur co‐doped carbon dots (N,S‐CDs) with a quantum yield of 16% were synthesized by a 1‐step hydrothermal method. The N,S‐CDs exhibited a good dispersion, with a graphite‐like structure, along with the fluorescence lifetime of approximately 7.50 ns. Findings showed that the fluorescence of the N,S‐CDs was effectively quenched by bovine hemoglobin as a result of the static fluorescence quenching. The mentioned quenching mechanism was investigated by the Stern‐Volmer equation, temperature‐dependent quenching, and fluorescence lifetime measurements. The binding constants, number of binding sites, and the binding average distance between the energy donor N,S‐CDs and acceptor bovine hemoglobin were calculated as well. These findings will provide for valuable insights on the future bioapplications of N,S‐CDs.
Respiratory syncytial virus–associated illness in adults with advanced chronic obstructive pulmonary disease and/or congestive heart failure
Authors: Falsey Ann R , Walsh Edward E , Esser Mark T , Shoemaker Kathryn , Yu Li , Griffin M Pam 
Published: August 2018

BackgroundRespiratory syncytial virus (RSV) is recognized as a serious pathogen in people with chronic cardiopulmonary conditions. Immunoprophylaxis might be considered for adults at high‐risk for frequent and severe RSV infection. Thus, we studied the incidence of RSV‐related medically attended acute respiratory illness (MARI) in adults with severe chronic obstructive pulmonary disease (COPD) and/or congestive heart failure (CHF).MethodsSubjects ≥50 years of age with Gold Class III/IV COPD and/or American Heart Association class III/IV CHF and exposure to children ≥once per month were recruited. Subjects were evaluated over 1.5 to 2.5 years for RSV‐associated MARI, defined as polymerase chain reaction (PCR) and/or seroresponse.ResultsFour hundred forty‐five subjects were enrolled between October 2011 and May 2012. Overall, 99 RSV infections were documented by PCR or serology for a cumulative incidence of 22.2%. Of these, 42 (9.4%) subjects had protocol‐specified RSV‐MARI for an incidence of 4.68/100 patient‐seasons. All‐cause MARI was common (63.85/100 patient‐seasons) with rhinovirus most commonly identified.ConclusionRSV infection was common in adults with severe COPD and/or advanced CHF. Given the severity of underlying cardiopulmonary diseases in the study population, most illnesses were surprisingly mild. Thus, active immunization rather than passive immunoprophylaxis with monoclonal antibodies may be a more cost‐effective strategy.
High prevalence and autochtonous transmission of human pegivirus (HPgV‐1) in blood donors in the extreme southern of Brazil
Authors: Da Mota Luísa D. , Finger‐Jardim Fabiana , Silva Cláudio M. , Germano Fabiana N. , Nader Maiba M. , Gonçalves Carla Vitola , Da Hora Vanusa P. , Silveira Jussara , Basso Rossana P. , Soares Marcelo A. , Martínez Ana M.B. 
Published: August 2018

Recent studies have suggested that human pegivirus 1 (HPgV‐1) may have some pathogenic potential. In the southernmost region of Brazil, studies on HPgV‐1 are scarce, and circulating genotypes have not yet been identified. The current study aimed to evaluate the prevalence of HPgV‐1 among blood donors from the southernmost region of Brazil and identify the genotypes involved with associated factors. A cross‐sectional study was conducted with 281 blood donors, who had their plasma subjected to RNA extraction, complementary DNA synthesis, HPgV‐1 detection by nested polymerase chain reaction, and subsequent genotyping. The observed prevalence of HPgV‐1‐RNA was 21.7%. The only variable that was significantly associated with virus infection was the relationship status of the donor. Single or no fixed partner blood donors were twice as likely to have HPgV‐1 (95% CI, 1.12 to 4.56; P = 0.02). Genotype 2—subtypes 2b (69%) and 2a (29%)—was the most prevalent. In the absence of risk factors for parenteral transmission, it is likely that sexual transmission was the route of infection in the individuals studied. Further work will be needed to determine whether this virus is inert in the population, or if there are potential deleterious effects in infected individuals.
The implication of CRISPR/Cas9 genome editing technology in combating human oncoviruses
Authors: Gilani Usman , Shaukat Memoona , Rasheed Arisha , Shahid Mehak , Tasneem Fareeda , Arshad Muhammad , Rashid Naeem , Shahzad Naveed 
Published: August 2018

AbstractIt is evidenced that 20% of all tumors in humans are caused by oncoviruses, including human papilloma viruses, Epstein‐Barr virus, Kaposi sarcoma virus, human polyomaviruses, human T‐lymphotrophic virus‐1, and hepatitis B and C viruses. Human immunodeficiency virus is also involved in carcinogenesis, although not directly, but by facilitating the infection of many oncoviruses through compromising the immune system. Being intracellular parasites with the property of establishing latency and integrating into the host genome, these viruses are a therapeutic challenge for biomedical researchers. Therefore, strategies able to target nucleotide sequences within episomal or integrated viral genomes are of prime importance in antiviral or anticancerous armamentarium. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) has emerged as a powerful genome editing tool. Standing out as a precise and efficient oncoviruses method, it has been extensively applied in recent experimental ventures in the field of molecular medicine, particularly in combating infections including tumor inducing viruses. This review is aimed at collating the experimental and clinical advances in CRISPR/Cas9 technology in terms of its applications against oncoviruses. Primarily, it will focus on the application of CRISPR/Cas9 in combating tumor viruses, types of mechanisms targeted, and the significant outcomes till date. The technical pitfalls of the CRISPR/Cas9 and the comparative approaches in evaluating this technique with respect to other available alternatives are also described briefly. Furthermore, the review also discussed the clinical aspects and the ethical, legal, and social issues associated with the use of CRISPR/Cas9.
Proactive career behaviors and subjective career success: The moderating role of national culture
Authors: Smale Adam , Bagdadli Silvia , Cotton Rick , Dello Russo Silvia , Dickmann Michael , Dysvik Anders , Gianecchini Martina , Kaše Robert , Lazarova Mila , Reichel Astrid , Rozo Paula , Verbruggen Marijke ,   Cross‐Cultural Collaboration on Contemporary Careers (5C) research collaborative
Published: August 2018

SummaryAlthough career proactivity has positive consequences for an individual's career success, studies mostly examine objective measures of success within single countries. This raises important questions about whether proactivity is equally beneficial for different aspects of subjective career success, and the extent to which these benefits extend across cultures. Drawing on Social Information Processing theory, we examined the relationship between proactive career behaviors and two aspects of subjective career success—financial success and work‐life balance—and the moderating role of national culture. We tested our hypotheses using multilevel analyses on a large‐scale sample of 11,892 employees from 22 countries covering nine of GLOBE's 10 cultural clusters. Although we found that proactive career behaviors were positively related to subjective financial success, this relationship was not significant for work‐life balance. Furthermore, career proactivity was relatively more important for subjective financial success in cultures with high in‐group collectivism, high power distance, and low uncertainty avoidance. For work‐life balance, career proactivity was relatively more important in cultures characterized by high in‐group collectivism and humane orientation. Our findings underline the need to treat subjective career success as a multidimensional construct and highlight the complex role of national culture in shaping the outcomes of career proactivity.
Systematic reviews and tech mining: A methodological comparison with case study
Authors: Anderson Patricia F. , Shannon Carol , Bickett Skye , Doucette Joanne , Herring Pamela , Kepsel Andrea , Lyons Tierney , McLachlan Scott , Wu Lin 
Published: August 2018

When the Medical Library Association identified questions critical for the future of the profession, it assigned groups to use systematic reviews to find the answers to these questions. Group 6, whose question was on emerging technologies, recognized early on that the systematic review process would not work well for this question, which looks forward to predict future trends, whereas the systematic review process looks back in time. We searched for new methodologies that were more appropriate to our question, developing a process that combined systematic review, text mining, and visualization techniques. We then discovered tech mining, which is very similar to the process we had created.In this paper, we describe our research design and compare tech mining and systematic review methodologies. There are similarities and differences in each process: Both use a defined research question, deliberate database selection, careful and iterative search strategy development, broad data collection, and thoughtful data analysis. However, the focus of the research differs significantly, with systematic reviews looking to the past and tech mining mainly to the future.Our comparison demonstrates that each process can be enhanced from a purposeful consideration of the procedures of the other. Tech mining would benefit from the inclusion of a librarian on their research team and a greater attention to standards and collaboration in the research project. Systematic reviews would gain from the use of tech mining tools to enrich their data analysis and corporate management communication techniques to promote the adoption of their findings.
Comparing the long‐term outcomes among patients with stomach and small intestine gastrointestinal stromal tumors: An analysis of the National Cancer Database
Authors: Giuliano Katherine , Ejaz Aslam , Reames Bradley N. , Choi WonSeok , Sham Jonathan , Gage Michele , Johnston Fabian M. , Ahuja Nita 
Published: August 2018

Background and ObjectivesGastrointestinal stromal tumors (GIST) are the most common sarcoma arising from the gastrointestinal tract. Data regrading long‐term prognosis based on tumor location (stomach vs small intestine) are mixed, so we aimed to analyze their outcomes using a large national oncology database.MethodsThe National Cancer Database was queried for cases of stomach and small intestine GIST between the years 2004 and 2014. Survival analysis was performed using the Kaplan‐Meier method, and factors related to survival were compared using the Cox proportional hazards model.ResultsOf 18 900 total patients, those with small intestine GIST had larger median tumor size (6.2 cm; interquartile range [IQR], 3.8 to 10.0 vs stomach: 5.0 cm; IQR, 3.0 to 9.0; P < 0.001) and a higher incidence of tumors with ≥5 mitoses/50 HPF (29.3% vs stomach: 24.2%; P < 0.001). Unadjusted median overall survival (OS) was longer for patients with stomach GIST (10.3 years) as compared to small intestine GIST (9.4 years) (P = 0.01). After controlling for patient and tumor‐related factors, however, OS did not differ between stomach and small intestine GIST (hazard ratio, 1.19; 95% confidence interval, 0.88 to 1.61; P = 0.26).ConclusionsPatients with small intestine GIST more commonly have larger, high mitotic rate tumors, but despite these worse prognostic features, tumor location did not independently impact OS.