bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional MRI registration with tissue‐specific patch‐based functional correlation tensors
Authors: Zhou Yujia , Zhang Han , Zhang Lichi , Cao Xiaohuan , Yang Ru , Feng Qianjin , Yap Pew‐Thian , Shen Dinggang 
Published: March 2018

AbstractPopulation studies of brain function with resting‐state functional magnetic resonance imaging (rs‐fMRI) rely on accurate intersubject registration of functional areas. This is typically achieved through registration using high‐resolution structural images with more spatial details and better tissue contrast. However, accumulating evidence has suggested that such strategy cannot align functional regions well because functional areas are not necessarily consistent with anatomical structures. To alleviate this problem, a number of registration algorithms based directly on rs‐fMRI data have been developed, most of which utilize functional connectivity (FC) features for registration. However, most of these methods usually extract functional features only from the thin and highly curved cortical grey matter (GM), posing great challenges to accurate estimation of whole‐brain deformation fields. In this article, we demonstrate that additional useful functional features can also be extracted from the whole brain, not restricted to the GM, particularly the white‐matter (WM), for improving the overall functional registration. Specifically, we quantify local anisotropic correlation patterns of the blood oxygenation level‐dependent (BOLD) signals using tissue‐specific patch‐based functional correlation tensors (ts‐PFCTs) in both GM and WM. Functional registration is then performed by integrating the features from different tissues using the multi‐channel large deformation diffeomorphic metric mapping (mLDDMM) algorithm. Experimental results show that our method achieves superior functional registration performance, compared with conventional registration methods.
Impacts of projected climate change on sediment yield and dredging costs
Authors: Dahl Travis A. , Kendall Anthony D. , Hyndman David W. 
Published: March 2018

AbstractChanges in climate may significantly affect how sediment moves through watersheds into harbours and channels that are dredged for navigation or flood control. Here, we applied a hydrologic model driven by a large suite of climate change scenarios to simulate both historical and future sediment yield and transport in two large, adjacent watersheds in the Great Lakes region. Using historical dredging expenditure data from the U.S. Army Corps of Engineers, we then developed a pair of statistical models that link sediment discharge from each river to dredging costs at the watershed outlet. Although both watersheds show similar slight decreases in streamflow and sediment yield in the near‐term, by Mid‐Century, they diverge substantially. Dredging costs are projected to change in opposite directions for the two watersheds; we estimate that future dredging costs will decline in the St. Joseph River by 8–16% by Mid‐Century but increase by 1–6% in the Maumee River. Our results show that the impacts of climate change on sediment yield and dredging may vary significantly by watershed even within a region and that agricultural practices will play a large role in determining future streamflow and sediment loads. We also show that there are large variations in responses across climate projections that cause significant uncertainty in sediment and dredging projections.
Development of a M cell‐targeted microparticulate platform, BSK02™, for oral immunization against the ovarian cancer antigen, sperm protein 17
Authors: Mattila Juha‐Pekka , Mirandola Leonardo , Chiriva‐Internati Maurizio 
Published: March 2018

AbstractAlthough it only accounts for approximately 5% of all female cancer cases, ovarian cancer (OC) ranks as the fifth leading cause of death due to cancer in women. We have evaluated the potential of an orally administered microparticulate vaccine incorporating an immunodominant epitope peptide derived from the cancer/testis antigen sperm protein 17 (SP17) aberrantly expressed in OC, to retard the progression of the disease. The peptide antigen and the immune‐stimulatory toll‐like receptor 9 ligand CpG oligonucleotide were incorporated into spray dried microparticles composed of enteric and sustained release polymers together with the Aleuria aurantia lectin targeting microfold cells present in the gut‐associated lymphoid tissue. These particles were administered via oral route to mice challenged week prior with SP17‐expressing ID8 OC cells. Analysis of splenocytes harvested from vaccinated mice revealed strong activation of IFN‐γ+/CD8+ lymphocytes in response to re‐stimulation with the SP17 antigen. Moreover, vaccinated animals showed significant retardation of ascites/tumor volume in comparison to placebo‐treated animals four weeks after the tumor challenge (p = 0.005). Taken together, our results suggest that vaccination against SP17 using orally administered microparticles could potentially be used as an effective consolidation strategy for OC patients with residual tumor or high probability for relapse following first‐line treatments. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 29–36, 2019.
Synthesis, Tautomerism, and Antiradical Activity of Novel Pteridinetrione Derivatives
Authors: Kazunin M. S. , Voskoboynik O. Yu. , Nosulenko I. S. , Berest G. G. , Sergeieva T. , Okovytyy S. , Karpenko O. V. , Priimenko B. O. , Kovalenko S. I. 
Published: March 2018

Synthesis of 1‐methyl‐6‐((2‐(aryl‐(heteryl‐))‐2‐oxoethyl) pteridine‐2,4,7(1H,3H,8H)‐triones via [4 + 2]‐cycloaddition of 1‐methyl‐5,6‐diaminouracil with ethyl 4‐aryl(heteryl)‐2,4‐dioxobutanoates is described in presented work. It was established that the reaction occurs regioselectively and proceeds under refluxing of starting compounds in acetic acid for 60 min. The structures of synthesized compounds were proven by complex of physicochemical methods including infrared, 1H‐, 13C‐NMR spectroscopy, liquid chromatography–mass spectrometry, and electron impact–mass spectrometry. Based on the detail analysis of the correlational NMR spectral data (correlation spectroscopy, heteronuclear single quantum coherence, heteronuclear multiple bond correlation, and Nuclear Overhauser effect spectroscopy), it was determined that in dimethyl sulfoxide solution, the 1‐methyl‐6‐((2‐(aryl‐(heteryl‐))‐2‐oxoethyl)pteridine‐2,4,7(1H,3H,8H)‐triones exist in two tautomeric forms: ketone (A) and enol (B). It was also found that tautomeric behavior of aforementioned compounds in hexadeuterated dimethyl sulfoxide is sensitive to the nature of the aryl or heteryl substituent at the position 6 of ring. The electron donating groups shift equilibrium to the tautomer A, while electron withdrawing – to the tautomer B. The synthesized compounds were tested on antiradical activity. It was found that obtained compounds reveal radical scavenging activity comparable or higher than ascorbic acid.
Brazilian network for HIV Drug Resistance Surveillance (HIV‐BresNet): a survey of treatment‐naive individuals
Authors: Arruda Monica B , Boullosa Lídia T , Cardoso Cynthia C , da Costa Carolina M , Brites Carlos , de Lima Shirlene TS , Kaminski Helena T , Aleixo Agdemir W , Esposito Ana OP , Cavalcanti Ana MS , Riedel Maristela , Couto‐Fernandez José C , Ferreira Selma B , de Oliveira Ivi CM , Portal Loreci E , Wolf Hilda HC , Fernandes Sandra B , de M. C. Pardini Maria I , Feiteiro Manoel VC , Tolentino Fernanda M , Diaz Ricardo S , Lopes Giselle ISL , Francisco Roberta BL , Véras Nazle MC , Pires Ana F , Franchini Miriam , Mesquita Fábio , Tanuri Amilcar ,   HIV‐BResNet
Published: March 2018

AbstractIntroductionIn Brazil, more than 487,450 individuals are currently undergoing antiretroviral treatment. In order to monitor the transmission of drug‐resistant strains and HIV subtype distribution in the country, this work aimed to estimate its prevalence and to characterize the nationwide pretreatment drug resistance in individuals recently diagnosed with HIV between 2013 and 2015.MethodsThe HIV threshold survey methodology (HIV‐THS, WHO) targeting antiretroviral‐naive individuals with recent HIV diagnosis was utilized, and subjects were selected from 51 highly populated cities in all five Brazilian macroregions. The HIV pol genotypic test was performed by genomic sequencing.ResultsWe analysed samples from 1568 antiretroviral‐naive individuals recently diagnosed with HIV, and the overall transmitted drug resistance (TDR) prevalence was 9.5% (150 sequences). The regional prevalence of resistance according to Brazilian geographical regions was 9.4% in the northeast, 11.2% in the southeast, 6.8% in the central region, 10.2% in the north and 8.8% in the south. The inhibitor‐specific TDR prevalence was 3.6% for nucleoside reverse transcriptase inhibitors (NRTIs), 5.8% for non‐nucleoside reverse transcriptase inhibitors (NNRTIs) and 1.6% for protease inhibitors (PIs); 1.0% of individuals presented resistance to more than one class of inhibitors. Overall, subtype B was more prevalent in every region except for the southern, where subtype C prevails.ConclusionsTo the best of our knowledge, this is the first TDR study conducted in Brazil with nationwide representative sampling. The TDR prevalence revealed a moderate rate in the five Brazilian geographical regions, although some cities presented higher TDR prevalence rates, reaching 14% in São Paulo, for example. These results further illustrate the importance of surveillance studies for designing future strategies in primary antiretroviral therapy, aiming to mitigate TDR, as well as for predicting future trends in other regions of the globe where mass antiretroviral (ARV) treatment was implemented.
Light‐Responsive Shape: From Micrometer‐Long Nanocylinders to Compact Particles in Electrostatic Self‐Assembly
Authors: Mariani Giacomo , Krieger Anja , Moldenhauer Daniel , Schweins Ralf , Gröhn Franziska 
Published: March 2018

AbstractA light‐triggered shape change of supramolecular nanostructures is achieved through electrostatically self‐assembly of linear polyelectrolytes and oppositely charged dyes in aqueous solution: Upon UV‐irradiation, 1‐µm‐long, flexible cylinders with a cross‐section of 10 nm convert into ellipsoids of 400 nm × 40 nm. The nano‐object shape is encoded in the molecular dye structure.
Biodiversity of fungi in hot desert sands
Authors: Murgia Manuela , Fiamma Maura , Barac Aleksandra , Deligios Massimo , Mazzarello Vittorio , Paglietti Bianca , Cappuccinelli Pietro , Al‐Qahtani Ahmed , Squartini Andrea , Rubino Salvatore , Al‐Ahdal Mohammed N. 
Published: March 2018

AbstractThe fungal community of six sand samples from Saudi Arabia and Jordan deserts was characterized by culture‐independent analysis via next generation sequencing of the 18S rRNA genes and by culture‐dependent methods followed by sequencing of internal transcribed spacer (ITS) region. By 18S sequencing were identified from 163 to 507 OTUs per sample, with a percentage of fungi ranging from 3.5% to 82.7%. The identified fungal Phyla were Ascomycota, Basal fungi, and Basidiomycota and the most abundant detected classes were Dothideomycetes, Pezizomycetes, and Sordariomycetes. A total of 11 colonies of filamentous fungi were isolated and cultured from six samples, and the ITS sequencing pointed toward five different species of the class Sordariomycetes, belonging to genera Fusarium (F. redolens, F. solani, F. equiseti), Chaetomium (C. madrasense), and Albifimbria (A. terrestris). The results of this study show an unexpectedly large fungal biodiversity in the Middle East desert sand and their possible role and implications on human health.
Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)
Authors: Kowalska Anna , Boruta Tomasz , Bizukojć Marcin 
Published: March 2018

AbstractThe application of microparticle‐enhanced cultivation (MPEC) is an attractive method to control mycelial morphology, and thus enhance the production of metabolites and enzymes in the submerged cultivations of filamentous fungi. Unfortunately, most literature data deals with the spore‐agglomerating species like aspergilli. Therefore, the detailed quantitative study of the morphological evolution of four different fungal species (Aspergillus terreus, Penicillium rubens, Chaetomium globosum, and Mucor racemosus) based on the digital analysis of microscopic images was presented in this paper. In accordance with the current knowledge, these species exhibit different mechanisms of agglomerates formation. The standard submerged shake flask cultivations (as a reference) and MPEC involving 10 μm aluminum oxide microparticles (6 g·L−1) were performed. The morphological parameters, including mean projected area, elongation, roughness, and morphology number were determined for the mycelial objects within the first 24 hr of growth. It occurred that heretofore observed and widely discussed effect of microparticles on fungi, namely the decrease in pellet size, was not observed for the species whose pellet formation mechanism is different from spore agglomeration. In the MPEC, C. globosum developed core‐shell pellets, and M. racemosus, a nonagglomerative species, formed the relatively larger, compared to standard cultures, pellets with distinct cores.
Reconstruction of diabetic lower leg and foot soft tissue defects using thoracodorsal artery perforator chimeric flaps
Authors: Sung Il Hoon , Jang Dong Woo , Kim Se Wan , Kim Youn Hwan , Kim Sang Wha 
Published: March 2018

AbstractBackgroundReconstruction of complicated diabetic lower leg and foot defects involving multiple tissue components remains a challenge. The purpose of this report is to introduce thoracodorsal artery perforator (TDAP) chimeric flaps for reconstructing diabetic lower leg and foot soft tissue defects.Patients and methodsBetween April 2010 and August 2016, 17 patients with multiple diabetic lower leg and foot defects underwent reconstruction with TDAP chimeric flaps. Nine were women and the mean age of the patients was 57.7 years (range 35–73 years). One patient had 3 separate defects, 14 patients had 2 separate defects, and 2 patients had defects with dead space. The size of the defects ranged from 5 × 3 cm to 20 × 10 cm.ResultsFifteen patients received TDAP chimeric flaps with two components (skin and muscle components), and two received three components (skin, latissimus dorsi (LD), and serratus anterior [SA] components). The skin paddle ranged from 10 × 3 cm to 25 × 14 cm. The LD components ranged from 3 × 5 cm to 20 × 10 cm and SA components ranged from 5 × 2 cm to 8 × 7 cm. All flaps survived except for partial loss of one muscle component. Four patients suffered postoperative complications including wound disruption and infection, all of which healed conservatively. The mean follow‐up was 31.3 months (range 8–60 months). Fifteen patients were able to walk, one patient walked with walker, and one patient who had amputation due to Charcot joint infection walked with prosthesis.ConclusionsThe TDAP chimeric flap may be another option for the complicated and complex wound coverage required to reconstruct diabetic lower leg and foot soft tissue defects.
PRO‐QUEST: a rapid assessment method based on progressive saturation for quantifying exchange rates using saturation times in CEST
Authors: Demetriou Eleni , Tachrount Mohamed , Zaiss Moritz , Shmueli Karin , Golay Xavier 
Published: March 2018

PurposeTo develop a new MRI technique to rapidly measure exchange rates in CEST MRI.MethodsA novel pulse sequence for measuring chemical exchange rates through a progressive saturation recovery process, called PRO‐QUEST (progressive saturation for quantifying exchange rates using saturation times), has been developed. Using this method, the water magnetization is sampled under non‐steady‐state conditions, and off‐resonance saturation is interleaved with the acquisition of images obtained through a Look‐Locker type of acquisition. A complete theoretical framework has been set up, and simple equations to obtain the exchange rates have been derived.ResultsA reduction of scan time from 58 to 16 minutes has been obtained using PRO‐QUEST versus the standard QUEST. Maps of both T1 of water and B1 can simply be obtained by repetition of the sequence without off‐resonance saturation pulses. Simulations and calculated exchange rates from experimental data using amino acids such as glutamate, glutamine, taurine, and alanine were compared and found to be in good agreement. The PRO‐QUEST sequence was also applied on healthy and infarcted rats after 24 hours, and revealed that imaging specificity to ischemic acidification during stroke was substantially increased relative to standard amide proton transfer–weighted imaging.ConclusionBecause of the reduced scan time and insensitivity to nonchemical exchange factors such as direct water saturation, PRO‐QUEST can serve as an excellent alternative for researchers and clinicians interested to map pH changes in vivo.
Possible effects of electroconvulsive therapy on refractory psychosis in primary progressive multiple sclerosis: A case report
Authors: Narita Zui , Satake Naoko , Sato Wakiro , Takano Harumasa 
Published: March 2018

AbstractIntroductionPatients with multiple sclerosis commonly show some degree of psychiatric symptoms. Primary progressive multiple sclerosis is a part of the spectrum of multiple sclerosis phenotypes with progressive accumulation of disability from disease onset and active course. Psychiatric symptoms are commonly shown in multiple sclerosis, and up to 10% of patients with multiple sclerosis have the primary progressive form. Thus, patients with primary progressive multiple sclerosis may also elicit psychiatric symptoms. However, little information is available on psychiatric symptoms, especially on psychosis, in primary progressive multiple sclerosis.CaseHere, we report on a 42‐year‐old woman with primary progressive multiple sclerosis whose psychosis did not respond to antipsychotics and was partially ameliorated by electroconvulsive therapy. She suffered from auditory hallucination, anxiety, depersonalization, and suicidal ideation. Initially, several antipsychotic agents were tried, but not effective. Given this, she underwent 12 sessions of electroconvulsive therapy.ConclusionOur observation suggests the possible utility of electroconvulsive therapy in the treatment of psychosis in primary progressive multiple sclerosis.
A randomized trial using telehealth technology to link caregivers with dementia care experts for in‐home caregiving support: FamTechCare protocol
Authors: Williams Kristine , Blyler Diane , Vidoni Eric D. , Shaw Clarissa , Wurth JoEllen , Seabold Denise , Perkhounkova Yelena , Van Sciver Angela 
Published: March 2018

ABSTRACTThe number of persons with dementia (PWD) in the United States is expected to reach 16 million by 2050. Due to the behavioral and psychological symptoms of dementia, caregivers face challenging in‐home care situations that lead to a range of negative health outcomes such as anxiety and depression for the caregivers and nursing home placement for PWD. Supporting Family Caregivers with Technology for Dementia Home Care (FamTechCare) is a multisite randomized controlled trial evaluating the effects of a telehealth intervention on caregiver well‐being and PWD behavioral symptoms. The FamTechCare intervention provides individualized dementia‐care strategies to in‐home caregivers based on video recordings that the caregiver creates of challenging care situations. A team of dementia care experts review videos submitted by caregivers and provide interventions to improve care weekly for the experimental group. Caregivers in the control group receive feedback for improving care based on a weekly phone call with the interventionist and receive feedback on their videos at the end of the 3‐month study. Using linear mixed modeling, we will compare experimental and control group outcomes (PWD behavioral symptoms and caregiver burden) after 1 and 3 months. An exploratory descriptive design will identify a typology of interventions for telehealth support for in‐home dementia caregivers. Finally, the cost for FamTechCare will be determined and examined in relation to hypothesized effects on PWD behavioral symptoms, placement rates, and caregiver burden. This research will provide the foundation for future research for telehealth interventions with this population, especially for families in rural or remote locations.
Manufacturing Nanoparticles with Orthogonally Adjustable Dispersibility in Hydrocarbons, Fluorocarbons, and Water
Authors: Zeininger Lukas , Stiegler Lisa M. S. , Portilla Luis , Halik Marcus , Hirsch Andreas 
Published: March 2018

AbstractWe describe a universal wet‐chemical shell‐by‐shell coating procedure resulting in colloidal titanium dioxide (TiO2) and iron oxide (Fe3O4) nanoparticles with dynamically and reversibly tunable surface energies. A strong covalent surface functionalization is accomplished by using long‐chained alkyl‐, triethylenglycol‐, and perfluoroalkylphosphonic acids, yielding highly stabilized core–shell nanoparticles with hydrophobic, hydrophilic, or superhydrophobic/fluorophilic surface characteristics. This covalent functionalization sequence is extended towards a second noncovalent attachment of tailor‐made nonionic amphiphilic molecules to the pristine coated core–shell nanoparticles via solvophobic (i.e. either hydrophobic, lipophobic, or fluorophobic) interactions. Thereby, orthogonal tuning of the surface energies of nanoparticles via noncovalent interactions is accomplished. As a result, this versatile bilayer coating process enables reversible control over the colloidal stability of the metal oxide nanoparticles in fluorocarbons, hydrocarbons, and water.
Translating Immunopeptidomics to Immunotherapy‐Decision‐Making for Patient and Personalized Target Selection
Authors: Fritsche Jens , Rakitsch Barbara , Hoffgaard Franziska , Römer Michael , Schuster Heiko , Kowalewski Daniel J. , Priemer Martin , Stos‐Zweifel Vlatka , Hörzer Helen , Satelli Arun , Sonntag Annika , Goldfinger Valentina , Song Colette , Mahr Andrea , Ott Martina , Schoor Oliver , Weinschenk Toni 
Published: March 2018

AbstractImmunotherapy is revolutionizing cancer treatment and has shown success in particular for tumors with a high mutational load. These effects have been linked to neoantigens derived from patient‐specific mutations. To expand efficacious immunotherapy approaches to the vast majority of tumor types and patient populations carrying only a few mutations and maybe not a single presented neoepitope, it is necessary to expand the target space to non‐mutated cancer‐associated antigens. Mass spectrometry enables the direct and unbiased discovery and selection of tumor‐specific human leukocyte antigen (HLA) peptides that can be used to define targets for immunotherapy. Combining these targets into a warehouse allows for multi‐target therapy and accelerated clinical application. For precise personalization aimed at optimally ensuring treatment efficacy and safety, it is necessary to assess the presence of the target on each individual patient's tumor. Here we show how LC‐MS paired with gene expression data was used to define mRNA biomarkers currently being used as diagnostic test IMADETECT™ for patient inclusion and personalized target selection within two clinical trials (NCT02876510, NCT03247309). Thus, we present a way how to translate HLA peptide presentation into gene expression thresholds for companion diagnostics in immunotherapy considering the peptide‐specific correlation to its encoding mRNA.