bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interlayer exchange coupling in ferromagnetic semiconductor trilayers with out-of-plane magnetic anisotropy
Authors: Chongthanaphisut Phunvira , Bac Seul-Ki , Choi Seonghoon , Lee Kyung Jae , Chang Jihoon , Choi Suho , Lee Sanghoon , Nnaji Moses , Liu X. , Dobrowolska M. , Furdyna J. K. 
Published: March 2019

AbstractWe report the observation of ferromagnetic (FM) and antiferromagnetic (AFM) interlayer exchange coupling (IEC) in GaMnAsP-based trilayer structures with out-of-plane magnetic anisotropy. Magnetization and anomalous Hall effect (AHE) measurements show well-resolved magnetization transitions corresponding to the two GaMnAsP layers. Minor loop measurements reveal a characteristic shift caused by IEC in all trilayer samples investigated. Interestingly, the FM IEC changes to AFM IEC for a trilayer with the thinnest (7 nm) top GaMnAsP layer as the temperature increases. The observation of temperature-induced transition of FM and AFM IEC in the same sample suggests the possibility of device applications by controlling the type of IEC in such GaMnAsP-based multilayers.
Compact Localized States in Engineered Flat-Band $${\mathscr{P}}{\mathscr{T}}$$ Metamaterials
Authors: Lazarides N. , Tsironis G. P. 
Published: March 2019

AbstractThe conditions leading to flat dispersionless frequency bands in truly one-dimensional parity-time ($${\mathscr{P}}{\mathscr{T}}$$
P
T
) symmetric metamaterials comprised of split‐ring resonators (SRRs) arranged in a binary pattern are obtained analytically. In this paradigmatic system, in which the SRRs are coupled through both electric and magnetic dipole-dipole forces, flat-bands may arise from tailoring its natural parameters (such as, e.g., the coupling coefficients between SRRs) and not from geometrical effects. For sets of parameters which values are tailored to flatten the upper band of the spectrum, the solution of the corresponding quadratic eigenvalue problem reveals the existence of compact, two-site localized eigenmodes. Numerical simulations confirm the existence and the dynamic stability of such modes, which can be formed through the evolution of single-site initial excitations without disorder or nonlinearity.
Unveiling high specific energy supercapacitor from layer-by-layer assembled polypyrrole/graphene oxide|polypyrrole/manganese oxide electrode material
Authors: Kulandaivalu Shalini , Suhaimi Nadhrah , Sulaiman Yusran 
Published: March 2019

AbstractA novel layer-by-layer (LBL) based electrode material for supercapacitor consists of polypyrrole/graphene oxide and polypyrrole/manganese oxide (PPy/GO|PPy/MnO2) has prepared by electrochemical deposition. The formation of LBL assembled nanocomposite is confirmed by Fourier transform infrared spectroscopy, Raman spectroscopy and X-ray diffraction. The field emission scanning electron microscopy images clearly showed that PPy/MnO2was uniformly coated on PPy/GO. The PPy/GO|PPy/MnO2symmetrical supercapacitor has revealed outstanding supercapacitive performance with a high specific capacitance of 786.6 F/g, an exceptionally high specific energy of 52.3 Wh/kg at a specific power of 1392.9 W/kg and preserve a good cycling stability over 1000 cycles. It is certain that PPy/GO|PPy/MnO2has an extraordinary perspective as an electrode for future supercapacitor developments. This finding contributes to a significant impact on the evolution of electrochemical supercapacitor.
Vitamin C deficiency causes muscle atrophy and a deterioration in physical performance
Authors: Takisawa Shoko , Funakoshi Tomoko , Yatsu Tomofumi , Nagata Kisaburo , Aigaki Toshiro , Machida Shuichi , Ishigami Akihito 
Published: March 2019

AbstractL-Ascorbic acid (AsA) is a water-soluble antioxidant. We examined the effect of AsA deficiency on skeletal muscle using senescence marker protein-30 (SMP30)-knockout (KO) mice that are defective in AsA biosynthesis, which makes this mouse model similar to humans, to clarify the function of AsA in skeletal muscle. Eight-week-old female SMP30-KO mice were divided into the following two groups: an AsA-sufficient group [AsA(+)] that was administered 1.5 g/L AsA and an AsA-deficient group [AsA(−)] that was administered tap (AsA-free) water. At 4 weeks, the AsA content in the gastrocnemius muscle of AsA(−) mice was 0.7% compared to that in the gastrocnemius muscle of AsA(+) mice. Significantly lower weights of all muscles were observed in AsA(−) mice than those in AsA(+) mice at 12 and 16 weeks. The cross-sectional area of the soleus was significantly smaller in AsA(−) mice at 16 weeks than that in AsA(+) mice. The physical performance of AsA(−) mice was significantly less than that of AsA(+) mice at 12 weeks. Following AsA deficiency for 12 weeks, the expression of ubiquitin ligases, such as atrogin1/muscle atrophy F-box (MAFbx) and muscle RING-finger protein 1 (MuRF1), was upregulated. Furthermore, all detected effects of AsA deficiency on muscles of the AsA(−) group at 12 weeks were restored following AsA supplementation for 12 weeks. Thus, longer-term AsA deficiency is associated with muscle wasting, that this can be reversed by restoring AsA levels.
In situ 10-cell RNA sequencing in tissue and tumor biopsy samples
Authors: Singh Shambhavi , Wang Lixin , Schaff Dylan L. , Sutcliffe Matthew D. , Koeppel Alex F. , Kim Jungeun , Onengut-Gumuscu Suna , Park Kwon-Sik , Zong Hui , Janes Kevin A. 
Published: March 2019

AbstractSingle-cell transcriptomic methods classify new and existing cell types very effectively, but alternative approaches are needed to quantify the individual regulatory states of cells in their native tissue context. We combined the tissue preservation and single-cell resolution of laser capture with an improved preamplification procedure enabling RNA sequencing of 10 microdissected cells. This in situ 10-cell RNA sequencing (10cRNA-seq) can exploit fluorescent reporters of cell type in genetically engineered mice and is compatible with freshly cryoembedded clinical biopsies from patients. Through recombinant RNA spike-ins, we estimate dropout-free technical reliability as low as ~250 copies and a 50% detection sensitivity of ~45 copies per 10-cell reaction. By using small pools of microdissected cells, 10cRNA-seq improves technical per-cell reliability and sensitivity beyond existing approaches for single-cell RNA sequencing (scRNA-seq). Detection of low-abundance transcripts by 10cRNA-seq is comparable to random 10-cell groups of scRNA-seq data, suggesting no loss of gene recovery when cells are isolated in situ. Combined with existing approaches to deconvolve small pools of cells, 10cRNA-seq offers a reliable, unbiased, and sensitive way to measure cell-state heterogeneity in tissues and tumors.
A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
Authors: Stringer Brett W. , Day Bryan W. , D’Souza Rochelle C. J. , Jamieson Paul R. , Ensbey Kathleen S. , Bruce Zara C. , Lim Yi Chieh , Goasdoué Kate , Offenhäuser Carolin , Akgül Seçkin , Allan Suzanne , Robertson Thomas , Lucas Peter , Tollesson Gert , Campbell Scott , Winter Craig , Do Hongdo , Dobrovic Alexander , Inglis Po-Ling , Jeffree Rosalind L. , Johns Terrance G. , Boyd Andrew W. 
Published: March 2019

AbstractLow-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation plus a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell (qimrberghofer.edu.au/q-cell/) and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM.
Effects of low and moderate refractive errors on chromatic pupillometry
Authors: Rukmini A. V. , Chew Milton C. , Finkelstein Maxwell T. , Atalay Eray , Baskaran Mani , Nongpiur Monisha E. , Gooley Joshua J. , Aung Tin , Milea Dan , Najjar Raymond P. 
Published: March 2019

AbstractChromatic pupillometry is an emerging modality in the assessment of retinal and optic nerve disorders. Herein, we evaluate the effect of low and moderate refractive errors on pupillary responses to blue- and red-light stimuli in a healthy older population. This study included 139 participants (≥50 years) grouped by refractive error: moderate myopes (>−6.0D and ≤−3.0D, n = 24), low myopes (>−3.0D and <−0.5D, n = 30), emmetropes (≥−0.5D and ≤0.5D, n = 31) and hyperopes (>0.5D and <6.0D, n = 54). Participants were exposed to logarithmically ramping-up blue (462 nm) and red (638 nm) light stimuli, designed to sequentially activate rods, cones and intrinsically-photosensitive retinal ganglion cells. Pupil size was assessed monocularly using infra-red pupillography. Baseline pupil diameter correlated inversely with spherical equivalent (R = −0.26, P < 0.01), and positively with axial length (R = 0.37, P < 0.01) and anterior chamber depth (R = 0.43, P < 0.01). Baseline-adjusted pupillary constriction amplitudes to blue light did not differ between groups (P = 0.45), while constriction amplitudes to red light were greater in hyperopes compared to emmetropes (P = 0.04) at moderate to bright light intensities (12.25–14.0 Log photons/cm²/s). Our results demonstrate that low and moderate myopia do not alter pupillary responses to ramping-up blue- and red-light stimuli in healthy older individuals. Conversely, pupillary responses to red light should be interpreted cautiously in hyperopic eyes.
Fossil evidence of elytra reduction in ship-timber beetles
Authors: Yamamoto Shûhei 
Published: March 2019

AbstractBeetles (Coleoptera) comprise about one quarter of all described animal species. One of the main contributors to their evolutionary success is the elytra, or hardened forewings, which have protective functions while maintaining their ability to fly. Unlike other beetles, some ship-timber beetles (Lymexylidae) have extremely small elytra and largely exposed functional hindwings. There is little fossil evidence illuminating the evolutionary history of short elytra in lymexylids. Here, I report five well-preserved lymexylid fossils in mid-Cretaceous and Cenozoic ambers from Myanmar (ca. 99 million years ago [Mya]), Russia (ca. 44 Mya), and the Dominican Republic (ca. 16 Mya). Three Cretaceous fossils have strongly reduced, shortened elytra, with unexpected variation in elytral size and shape, whereas very small, modified elytra are found only in much younger Dominican amber. These morphologically diverse extinct lymexylids shed new light on the early origin and evolutionary history of elytra reduction and its diverse variation in the ship-timber beetles. Based on the striking morphological similarities with extant lymexylids, these extinct taxa might have had the same, or similar, ecological, behavioural, and flight modes as the extant ship-timber beetles.
The use of Leaf Surface Contact Cues During Oviposition Explains Field Preferences in the Willow Sawfly Nematus oligospilus
Authors: Fernández Patricia C. , Braccini Celina L. , Dávila Camila , Barrozo Romina B. , Aráoz M. Victoria Coll , Cerrillo Teresa , Gershenzon Jonathan , Reichelt Michael , Zavala Jorge A. 
Published: March 2019

AbstractAfter an insect herbivore has reached its host plant, contact cues from the leaf surface often determine host acceptance. We studied contact cues during oviposition behavior of a willow pest, the sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae), a specialist feeder on Salix (Salicaceae) trees, and how it determines oviposition preference in lab and field conditions. We described the sequence of behaviors that lead to egg laying on the most and least preferred willow species. Then we studied the morphology of chemosensory structures present on the female antenna, cerci and ovipositor. Since phenolic glycosides (PGs) are the main secondary metabolites present in Salicaceae species, we investigated their role in host acceptance. We quantified these compounds in different willow species and correlated PG content with oviposition preference under lab and natural field conditions. We demonstrated a major role for contact cues in triggering N. oligospilus egg laying on the leaf surface of preferred willow genotypes. Firstly cues are sensed by antennae, determining to leave or stay on the leaf. After that, sensing is performed by abdominal cerci, which finally triggers egg laying. The lack of PGs in non-preferred species and the significant correlation observed between PGs, natural damage and oviposition preference suggest a role for these compounds in host selection. Our study suggests that in specialist feeders, secondary compounds normally acting as defenses can actually act as a susceptibility factor by triggering specific insect behavior for oviposition. These defensive compounds could be selected against to increase resistance.
Electrophysiological correlates of top-down attentional modulation in olfaction
Authors: Singh Archana K. , Touhara Kazushige , Okamoto Masako 
Published: March 2019

AbstractThe capacity to pay attention is important for the cognitive ability, for example, evaluating an object for its qualities. Attention can selectively prioritize the neural processes that are relevant to a given task. Neuroimaging investigations on human attention are primarily focused on vision to the exclusion of other sensory systems, particularly olfaction. Neural underpinnings of human olfactory attention are still not clearly understood. Here, we combined electroencephalographic measurements of olfactory event related potential with electrical neuroimaging to investigate how the neural responses after inhaling the same odor differ between conditions with varying levels of attention, and, in which brain areas. We examined the neural responses when participants attended to a rose-like odor of phenylethyl alcohol for evaluating its pleasantness versus its passive inhalation. Our results gathered significant evidence for attentional modulation of the olfactory neural response. The most prominent effect was found for the late positive component, P3, of olfactory event related potential within a second from the odor onset. The source reconstruction of this data revealed activations in a distributed network of brain regions predominantly in inferior frontal cortex, insula, and inferior temporal gyrus. These results suggest that the neuronal modulations from attention to olfactory pleasantness may be subserved by this network.
Phosphorylation Regulates CAP1 (Cyclase-Associated Protein 1) Functions in the Motility and Invasion of Pancreatic Cancer Cells
Authors: Wu Huhehasi , Hasan Rokib , Zhang Haitao , Gray Joshua , Williams Dominic , Miller Morgan , Allen Faith , Lee Virlan , Kelly Thomas , Zhou Guo-Lei 
Published: March 2019

AbstractPancreatic cancer has the worst prognosis among major malignancies, largely due to its highly invasive property and difficulty in early detection. Mechanistic insights into cancerous transformation and especially metastatic progression are imperative for developing novel treatment strategies. The actin-regulating protein CAP1 is implicated in human cancers, while the role still remains elusive. In this study, we investigated roles for CAP1 and its phosphor-regulation in pancreatic cancer cells. No evidence supports remarkable up-regulation of CAP1 in the panel of cancer cell lines examined. However, knockdown of CAP1 in cancer cells led to enhanced stress fibers, reduced cell motility and invasion into Matrigel. Phosphorylation of CAP1 at the S308/S310 tandem regulatory site was elevated in cancer cells, consistent with hyper-activated GSK3 reported in pancreatic cancer. Inhibition of GSK3, a kinase for S310, reduced cell motility and invasion. Moreover, phosphor mutants had defects in alleviating actin stress fibers and rescuing the reduced invasiveness in the CAP1-knockdown PANC-1 cells. These results suggest a required role for transient phosphorylation for CAP1 function in controlling cancer cell invasiveness. Depletion of CAP1 also reduced FAK activity and cell adhesion, but did not cause significant alterations in ERK or cell proliferation. CAP1 likely regulates cancer cell invasiveness through effects on both actin filament turnover and cell adhesion. Finally, the growth factor PDGF induced CAP1 dephosphorylation, suggesting CAP1 may mediate extracellular signals to control cancer cell invasiveness. These findings may ultimately help develop strategies targeting CAP1 or its regulatory signals for controlling the invasive cycle of the disease.
The genes expression difference between winged and wingless bird cherry-oat aphid Rhopalosiphum padi based on transcriptomic data
Authors: Zhang Rong-Jiao , Chen Jing , Jiang Li-Yun , Qiao Ge-Xia 
Published: March 2019

AbstractAphids produce wing and wingless morphs, depending on the environmental conditions during their complex life cycles. Wing and wingless variations play an important role in migration and host alternation, affecting the migration and host alternation processes. Several transcriptional studies have concentrated on aphids and sought to determine how an organism perceives environmental cues and responds in a plastic manner, but the underlying mechanisms have remained unclear. Therefore, to better understand the molecular mechanisms underlying the wing polyphenism of this fascinating phenomenon, we provide the first report concerning the wing development of aphids in bird cherry-oat aphid Rhopalosiphum padi with comparative transcriptional analysis of all the developmental stages by RNA-Seq. We identified several candidate genes related to biogenic amines and hormones that may be specifically involved in wing development. Moreover, we found that the third instar stage might be a critical stage for visibility of alternative morphs as well as changes in the expression of thirty-three genes associated with wing development. Several genes, i.e., Wnt2, Fng, Uba1, Hh, Foxo, Dpp, Brk, Ap, Dll, Hth, Tsh, Nub, Scr, Antp, Ubx, Asc, Srf and Fl, had different expression levels in different developmental stages and may play important roles in regulating wing polyphenism.
Construction of non-canonical PAM-targeting adenosine base editors by restriction enzyme-free DNA cloning using CRISPR-Cas9
Authors: Jeong You Kyeong , Yu Jihyeon , Bae Sangsu 
Published: March 2019

AbstractMolecular cloning is an essential technique in molecular biology and biochemistry, but it is frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 endonucleases can induce site-specific DNA double-strand breaks at sites homologous to their guide RNAs, rendering an alternative to restriction enzymes. Here, by combining DNA cleavage via a Cas9 endonuclease and DNA ligation via Gibson assembly, we demonstrate a precise and practical DNA cloning method for replacing part of a backbone plasmid. We first replaced a resistance marker gene as a proof of concept and next generated DNA plasmids that encode engineered Cas9 variants (VQR, VRER and SpCas9-NG), which target non-canonical NGA, NGCG and NG protospacer-adjacent motif (PAM) sequences, fused with adenosine deaminases for adenine base editing (named VQR-ABE, VRER-ABE and NG-ABE, respectively). Ultimately, we confirmed that the re-constructed plasmids can successfully convert adenosine to guanine at endogenous target sites containing the non-canonical NGA, NGCG and NG PAMs, expanding the targetable range of the adenine base editing.
Characterization of the gut microbiota of invasive Agrilus mali Matsumara (Coleoptera: Buprestidae) using high-throughput sequencing: uncovering plant cell-wall degrading bacteria
Authors: Bozorov Tohir A. , Rasulov Bakhtiyor A. , Zhang Daoyuan 
Published: March 2019

AbstractThe genusAgriluscomprises diverse exotic and agriculturally important wood-boring insects that have evolved efficient digestive systems.Agrilus maliMatsumara, an invasive insect, is causing extensive mortality to endangered wild apple trees in Tianshan. In this study, we present an in-depth characterization of the gut microbiota ofA. malibased on high-throughput sequencing of the 16S rRNA gene and report the presence of lignocellulose-degrading bacteria. Thirty-nine operational taxonomic units (OTUs) were characterized from the larval gut. OTUs represented 6 phyla, 10 classes, 16 orders, 20 families, and 20 genera. The majority of bacterial OTUs belonged to the order Enterobacteriales which was the most abundant taxa in the larval gut. Cultivable bacteria revealed 9 OTUs that all belonged to Gammaproteobacteria. Subsequently, we examined the breakdown of plant cell-wall compounds by bacterial isolates. Among the isolates, the highest efficiency was observed inPantoeasp., which was able to synthesize four out of the six enzymes (cellulase, cellobiase, β-xylanase, and β-gluconase) responsible for plant-cell wall degradation. One isolate identified asPseudomonas orientalisexhibited lignin peroxidase activity. Our study provides the first characterization of the gut microbial diversity ofA. malilarvae and shows that some cultivable bacteria play a significant role in the digestive tracts of larvae by providing nutritional needs.
Graphene-coated microballs for a hyper-sensitive vacuum sensor
Authors: Il Ahn Sung , Kim Yong Woo , Lee Seong Eui , Kim Minjun , Choi Kyeong-Keun , Park Jung-Chul 
Published: March 2019

AbstractReduced graphene oxide (RGO)-coated microballs of poly (methyl methacrylate) (PMMA) used for fabricating three-dimensional sensor (3D sensor), which are expected to exhibit high sensitivity compared with conventional two-dimensional (2D) sensors, were prepared using a reaction-based assembly process. The sheet resistance and transmittance of the RGO-coated balls decreased with increasing number of coatings, implying that the RGO was well adhered to the ball by the assembly method. Two types of vacuum pressure sensors using multiple balls and a single ball were fabricated using lift-off and air-blowing methods, respectively. At pressures <1 torr, the sensors showed an increased resistance value due to the bending of graphene sheets by the Van der Waals attractive force. Further, the pressure versus resistance values at the logarithmic scale showed a linear relation, with a pressure reading error <6%. Compared with the 2D sensor fabricated using RGO, the multiball sensor exhibited almost 4–5 times higher RRC value. The single-ball sensor showed reasonable reproducibility at various temperatures. Given the size and pressure reading range of the sensor, the sensitivity of the single-ball sensor at 100 °C was approximately 6,000 times greater than that of the sensor with the highest sensitivity reported in the literature. The increase in surface area and the geometric effect of the sensing part of the single-ball sensor appeared to be responsible for its abnormally high sensitivity.