bluuebunny/crossref_metadata_2025_split huggingface dataset:
Degradation of CFTR-F508del By the Ubiquitin E2 Conjugating Enzyme UBE 2L6 and the E3 Ligase RNF19B
Authors: Rajagopalan Carthic , Wei Hongguang , Hou Xia , Bouhamdan Mohamad , Sun Fei 
Published: July 2019

Abstract
Background

Cystic Fibrosis (CF) is the most common, lethal autosomal-recessive disorder, and is caused by mutations in the cystic fibrosis transmembrane conductance regulator protein (CFTR), an anion channel that is found in epithelial cells lining multiple organs. The most common mutation of CFTR is deletion of phenylalanine at position 508 (CFTR-F508del), which produces a misfolded protein. This misfolded protein is confined in the endoplasmic reticulum (ER), ubiquitinated and signaled for degradation via the cytosolic proteasome. Previous studies demonstrating experimental restoration of CFTR-F508del (F508del) trafficking to the plasma membrane showed partial function of the chloride channel, raising therapeutic speculations. However, many mechanisms that underly its degradation is still unclear.

Results

We discovered that the E2 conjugating enzyme, UBE 2L6, aids in the degradation of F508del. We also show that UBE 2L6’s interacting E3 ligase, RNF19B, also assists in F508del degradation. We used siRNA-mediated silencing of endogenous UBE 2L6 and RNF19B in the CF human bronchial epithelial (HBE) cell line CFBE-F508del to demonstrate that there is an increase in F508del-CFTR expression. We also co-expressed UBE 2L6 and RNF 19B with F508del in HEK293 cells that demonstrated a decrease in F508del compared to control. Cycloheximide-chase (CHX) experiments using HEK 293 cells overexpressing RNF19B and F508del showed that there was a decrease in F508del half-life. Lastly, using siRNA-mediated silencing of endogenous UBE 2L6 and RNF19B in CFBE-F508del cells increased forskolin-stimulated short-circuit currents. Interestingly, there seemed to be cooperatively larger increases in short-circuit currents in CFBE-F508del cells that were silenced with UBE 2L6 or RNF19B were treated for 24 hours with VX-809.

Conclusion

These data suggest that there is an additional mechanism where the E2 conjugating enzyme, UBE 2L6, and the E3 ubiquitin ligase, RNF19B, is responsible for the ubiquitination and subsequent proteasomal degradation of the misfolded F508del. This study presents a novel therapeutic strategy to synergistically restore the function of F508del.
Knowledge and practice of immediate new-born care among midwives in governmental health facilities of central zone, Tigray region, north Ethiopia: cross sectional study
Authors: Gebru Tesfay Tsegay , Murugan Rajalakshmi , Abrha Alem Gebremariam , Goyteom Mekonnen Haftom 
Published: July 2019

Abstract
Objective

The objective of this study was to assess knowledge and practice of immediate newborn care among midwives in governmental health facilities of central zone of Tigray regional state, 2016.

Results

The mean age of the study participants was 34.1 years. Majority of the participants (83%) were diploma midwives. The score of knowledge of participants on immediate newborn care was 17.7% good and 25.2% poor. More than half (52.4%) of midwives practiced immediate newborn care. Midwives working in health center have 82% lower odds of newborn care compared to those working in hospital (p=0.000, OR=0.18 (0.07, 0.43).
MicroRNAs-mRNAs Expression Profile and Their Potential Role in Cadmium Stress Response in Brassica napus
Authors: Fu Ying , Mason Annaliese S. , Zhang Yaofeng , Lin Baogang , Fu Donghui , Yu Huasheng 
Published: July 2019

Abstract
Background: Oilseed rape is an excellent candidate for phytoremediation of cadmium-contaminated soils given its advantages of high biomass, fast growth, moderate metal accumulation, ease of harvesting, and metal tolerance, but the cadmium response pathways in this species (Brassica napus) have yet to be fully elucidated. A combined analysis of miRNA and mRNA expression data to infer Cd-induced transcriptional regulation has not been previously been reported in B. napus.
Results: In the current study, we characterized concurrent changes in miRNA and mRNA profiles in the roots and shoots of B. napus seedlings after 10 days of 10 mg/L Cd2+ treatment. In total, 39 miRNAs belonging to 14 families were identified in the root, and 53 miRNAs belonging 16 miRNA families were identified in the shoot, of which the miR397, miR395, miR408, miR398 and miR858 families showed the most significant expression changes in response to Cd stress. A further 399 in the root and 389 differentially expressed genes (DEGs) in the shoot were identified, with very little overlap between tissue types. A total of 24 root and 16 shoot anti-regulation miRNA-mRNA interaction pairs (up-regulation of miRNA and down-regulation of mRNA or vice-versa) were identified in response to Cd, which involved in the key pathways of palnt stress response, e.g. four genes targeted by one miRNA were involved in a pathway for detoxification of superoxide radicals. Cd stress significantly impacted the photosynthetic pathway. We identified transcription factor activation, antioxidant response pathways and secondary metabolic processes such as glutathione (GSH) and phenylpropanoid metabolism as major components in the response to Cd-induced stress in both roots and shoots.
Conclusions: miRNAs and mRNAs showed concurrent expression changes for cadmium response in Brassica napus. The leaves and roots showed several overlap of differentially expressed miRNAs but very little overlap of differentially expressed mRNAs for Cd response. Several anti-regulation miRNA-mRNA interaction pairs were identified, which involved in the key pathways of plant stress response like detoxification of superoxide radicals. Photosynthetic pathway, transcription factor activation, antioxidant response and secondary metabolic processes were major components for cadmium response.
Factors Influencing Adherence to the New Intermittent Preventive Treatment of Malaria in Pregnancy Policy in Keta District of the Volta Region, Ghana
Authors: Vandy Alren O. , Peprah Nana Yaw , Jerela Joseph Yaw , Titiati Perfect , Manu Abubakar , Akamah Joseph , Maya Ernest T. , Torpey Kwasi 
Published: July 2019

Abstract
Background

About 25% of pregnant women in malaria endemic areas are infected with malaria and this accounts for about 15% of maternal death globally. Intermittent preventive treatment in pregnancy with sulfadoxine-pyrimethamine (IPTp-SP) is one of the main strategies for prevention of malaria in pregnancy. A new recommendation was made by World Health Organization (WHO) that at least three doses of IPTp-SP should be administered before delivery. This study sought to determine the factors influencing adherence to the new IPTp-SP policy in Keta District, Volta region, Ghana.

Methods

A cross-sectional quantitative study among 375 nursing mothers at four selected health facilities in Keta district, Ghana was conducted using a structured questionnaire to interview participants. Data was analyzed using STATA 15. Chi-square was used to test bivariate association between categorical variables and adherence. Logistic regression analysis was used to examine sociodemographic, individual and institutional factors influencing adherence to IPTp-SP.

Result

About 82.1% of participants adhered to the WHO policy recommendations of at least three doses of IPTp-SP. However, only 17.1% received Ghana’s five dose coverage recommendation. The proportion of IPTp-SP coverage for IPTp1 was 98.9%; IPTp2 95.5%; IPTp3 80.8%; IPTp4 39.5%; IPTp5 17.1%.

Conclusion

Adherence to IPTp-SP was satisfactory according to WHO’s policy recommendation, however, majority of the participants had less than the five doses recommended in Ghana. Number of ANC visits and knowledge of malaria were the main determinants of adherence to IPTp-SP.
A CRISE E O PROCESSSO DE CONSTITUCIONALIZAÃ‡ÃƒO DO DIREITO ADMINISTRATIVO BRASILEIRO.
Authors: Reis JoÃ£o Emilio de Assis 
Published: July 2019

Â O presente artigo Ã© um ensaio teÃ³rico acerca da crise do direito administrativo e o seu processo de constitucionalizaÃ§Ã£o. Partindo da revisÃ£o histÃ³rica do surgimento do direito administrativo, busca contextualizar o surgimento de alguns dos seus principais institutos, cuja prÃ³pria origem termina por gerar alguns dos principais problemas do direito administrativo contemporÃ¢neo, visto que algumas de suas premissas na sua feiÃ§Ã£o tradicional no Estado Liberal podem se mostrar incompatÃ­veis com o papel de centralidade no ordenamento dado aos direitos fundamentais pela ConstituiÃ§Ã£o Federal de 1988.
GRAVIDEZ NA ADOLESCÃŠNCIA: ESTUDO EPIDEMIOLÃ“GICO REALIZADO NA CIDADE DE MANHUAÃ‡U - MG
Authors: Sucasas Humberto Tostes de Faria , Ribeiro Guilherme Vieira Borchio , Sales Emanuel Costa , Flora Heytor dos Santos , Da Silva Juliana Santiago 
Published: July 2019

A gravidez na adolescÃªncia Ã© considerada por muitos como um problema de saÃºde pÃºblica. No Brasil os Ã­ndices de fecundidade, que Ã© o nÃºmero de crianÃ§as nascidas por mulher, vem diminuindo, mas mesmo com o aumento da divulgaÃ§Ã£o dos mÃ©todos contraceptivos, o nÃºmero de registros de adolescentes grÃ¡vidas Ã© algo que chama atenÃ§Ã£o. O presente artigo visa mostrar uma nova concepÃ§Ã£o sobre a temÃ¡tica. Para isso foi realizado um estudo epidemiolÃ³gico na cidade de ManhuaÃ§u com o objetivo de comparar os dados coletados, relacionados ao tema, nas esferas municipal e nacional, podendo servir de embasamento para futuras pesquisas que visem intervenÃ§Ãµes neste Ã¢mbito. Foram elaborados grÃ¡ficos que ilustraram uma proximidade entre os dados do municÃ­pio e os do paÃ­s. Todavia foi observado um nÃºmero maior de adolescentes grÃ¡vidas na cidade em estudo.Â Algumas concepÃ§Ãµes nÃ£o consideram apenas a baixa idade materna como um determinante de adversidades. Deste modo, o que as causariam seriam fatores socioeconÃ´micos e culturais, os quais acometem com maior frequÃªncia as jovens, e por isso Ã© feita uma errÃ´nea associaÃ§Ã£o.
Wastewater treatment from the biodiesel production using waste cooking oil by electrocoagulation: a multivariate approach
Authors: Sari-Erkan Hanife 
Published: July 2019

Abstract
This study mainly focuses on the process of electrocoagulation (EC) for the wastewater treatment from biodiesel production using waste cooking oil. The effects of current density, initial pH and electrolysis time on the EC process using aluminum (Al) and iron (Fe) electrodes were investigated for removal of chemical oxygen demand (COD). The COD removal efficiencies were found to be 62.7% and 63.4% at optimum conditions for Al (current density: 43 mA/cm2, pH: 5, time: 21 min) and Fe (current density: 47 mA/cm2, pH: 7.7, time: 30 min) electrodes, respectively. At these optimum conditions, the removal efficiencies of oil & grease, total phosphorus (TP), orthophosphate (PO4-P) and total suspended solids (TSS) were determined respectively to be above 89.9%, 98.9%, 99.5%, 86.7% for Al electrodes and 90.8%, 98.5%, 97.6%, 89.6% for Fe electrodes. Total operating costs were also found to be 6.43 €/m3 and 7.01 €/m3 for Al and Fe electrodes, respectively. The results indicate that the EC process using both types of electrodes seems to ensure an efficient treatment of biodiesel wastewater in terms of oil & grease and TP.
Análise da receita per capita nos municípios catarinenses conforme a receita corrente líquida
Authors: Zolet Roberto , Scheren Gilvane , Galante Celso 
Published: July 2019

Objetivo: A pesquisa objetivou identificar a receita per capita nos municípios catarinenses com base na Receita Corrente Líquida e a relação existente entre receitas próprias, transferências com a capacidade de cada município.

Metodologia: O estudo que ora se apresenta, quanto aos objetivos como descritiva, pois busca analisar a receita pública per capita, analogamente aos procedimentos caracteriza-se como documental, pois torna possível, a partir dos relatórios publicados, em relação à abordagem do problema, como pesquisa quantitativa, com emprego de valores e suas correlações. O período em análise compreende os anos de 2015 e 2016. A amostra consiste nos 295 municípios catarinenses.

Resultados: Os resultados apontam que, na estratificação e análise, a Receita Corrente Líquida Per Capita por porte de Município, aos quais, os municípios com maior porte possuem, proporcionalmente, melhor distribuição da arrecadação municipal, que culmina na maior capacidade de enfrentamento às demandas sociais. Na média da Receita Corrente Líquida Per Capita dos municípios Catarinenses, nos anos de 2015 e 2016, foi de R$ 3.332,61, com uma variação entre a receita per capita mínima e máxima de R$ 1.377,24 e R$ 8.055,16, o que demonstra haver discrepância significativa entre os municípios analisados.

Contribuições do Estudo: Considerando os valores recebidos pelos municípios catarinenses, a pesquisa procurou demonstrar e esclarecer a influência de valores recebidos da União e do Estado por ente para investimentos e manutenção das atividades, em função da divisão populacional e porte de cada município, sendo informações uteis e relevantes para o planejamento e captação de recursos para aplicação em benefício da população.
Representatividade dos incentivos fiscais da Lei de Informática no resultado econômico de indústrias catarinenses
Authors: Almeida Dalci Mendes , Pereira Ian Mota 
Published: July 2019

Objetivo: O complexo eletrônico é um setor que está em constante desenvolvimento no país. O governo busca por meio de políticas governamentais estimular o seu crescimento, criando uma série de incentivos às empresas do setor de tecnologia, que investem em pesquisa e desenvolvimento, como a Lei de Informática. Desse modo, o objetivo da pesquisa consiste em verificar a representatividade dos incentivos fiscais da Lei de Informática no resultado econômico de empresas catarinenses.
Metodologia: A pesquisa é classificada como descritiva, de levantamento e quantitativa. A população foi formada por 64 empresas, que utilizam dos incentivos fiscais da Lei de Informática, cadastradas no Ministério da Ciência, Tecnologia, Inovações e Comunicações em 2014, sendo a amostra representada por 18 empresas. Os dados foram analisados por meio de estatística descritiva.
Resultados: Os achados da pesquisa apontaram que, a renúncia dos impostos representou valores consideráveis no resultado econômico das empresas, em que 44,44% afirmaram que a renúncia proveniente dos incentivos fiscais, representam mais de 2% sobre o resultado econômico (lucro).
Contribuições do Estudo: A pesquisa traz contribuições teóricas, visto a pouca exploração de estudos sobre a Lei da Informática nessa vertente; e práticas, tendo em vista que apresenta esclarecimentos quanto as prerrogativas para concessão do incentivo fiscal, além de números que podem auxiliar as empresas em projeções orçamentárias a partir da concessão do benefício, reduzindo seu ônus tributário e, por consequência, os custos de sua atividade, tornando-se mais competitivas e consolidadas no mercado.
The Effect of Robot Attentional Behaviors on User Perceptions and Behaviors in a Simulated Health Care Interaction: Randomized Controlled Trial
Authors: Johanson Deborah L , Ahn Ho Seok , MacDonald Bruce A , Ahn Byeong Kyu , Lim JongYoon , Hwang Euijun , Sutherland Craig J , Broadbent Elizabeth 
Published: July 2019

Background
For robots to be effectively used in health applications, they need to display appropriate social behaviors. A fundamental requirement in all social interactions is the ability to engage, maintain, and demonstrate attention. Attentional behaviors include leaning forward, self-disclosure, and changes in voice pitch.

Objective
This study aimed to examine the effect of robot attentional behaviors on user perceptions and behaviors in a simulated health care interaction.

Methods
A parallel randomized controlled trial with a 1:1:1:1 allocation ratio was conducted. We randomized participants to 1 of 4 experimental conditions before engaging in a scripted face-to-face interaction with a fully automated medical receptionist robot. Experimental conditions included a self-disclosure condition, voice pitch change condition, forward lean condition, and neutral condition. Participants completed paper-based postinteraction measures relating to engagement, perceived robot attention, and perceived robot empathy. We video recorded interactions and coded for participant attentional behaviors.

Results
A total of 181 participants were recruited from the University of Auckland. Participants who interacted with the robot in the forward lean and self-disclosure conditions found the robot to be significantly more stimulating than those who interacted with the robot in the voice pitch or neutral conditions (P=.03). Participants in the forward lean, self-disclosure, and neutral conditions found the robot to be significantly more interesting than those in the voice pitch condition (P<.001). Participants in the forward lean and self-disclosure conditions spent significantly more time looking at the robot than participants in the neutral condition (P<.001). Significantly, more participants in the self-disclosure condition laughed during the interaction (P=.01), whereas significantly more participants in the forward lean condition leant toward the robot during the interaction (P<.001).

Conclusions
The use of self-disclosure and forward lean by a health care robot can increase human engagement and attentional behaviors. Voice pitch changes did not increase attention or engagement. The small effects with regard to participant perceptions are potentially because of the limitations in self-report measures or a lack of comparison for most participants who had never interacted with a robot before. Further research could explore the use of self-disclosure and forward lean using a within-subjects design and in real health care settings.
An E-Learning Program for Increasing Physical Activity Associated Behaviors Among People with Spinal Cord Injury: Usability Study
Authors: Wilroy Jereme D , Martin Ginis Kathleen A , Rimmer James H , Wen Huacong , Howell Jennifer , Lai Byron 
Published: July 2019

Background
The majority of people with spinal cord injury (SCI) in the United States are not meeting the recommended guidelines for regular physical activity. Behavior change techniques (eg, goal setting and action planning) that are framed within the principles of the social cognitive theory (self-efficacy and self-regulation) have the potential to enhance physical activity behavior.

Objective
The aim of the study was to develop and test the usability of an electronic learning (e-learning) program for improving social cognitive factors related to physical activity behavior among people with SCI.

Methods
The program was created through an iterative process of development and refinement, using a modification of a similar methodology used to develop evidence-informed guidelines in health promotion for people with disabilities (Guidelines, Recommendations, and Adaptations Including Disability; GRAIDs framework). The study included 4 phases: (1) initial product creation, (2) national survey, (3) expert review, and (4) usability testing. Usability testing included both quantitative and qualitative data collection and analyses.

Results
The review of the program by an expert panel (n=5) and the results from a national survey (n=142) led to several refinements. Usability testing demonstrated that the program could be completed in a timely manner (<30 min). Participants reported 5 themes: (1) the program improves social cognitions related to physical activity participation; (2) reflection of physical activity behavior; (3) positive perceptions of the quality of the program; (4) positive perceptions of the program operation and effectiveness; and (5) recommendations for improvement. Each item was incorporated into a revised program version 1.0.

Conclusions
This study incorporated an evidence-based framework for developing a brief 30-min e-learning program for increasing the physical activity behavior among people with SCI. The Exercise Strategies Through Optimized Relevant Interactive E-learning Storytelling (e-STORIES) program could be completed in a timely manner and was reported by participants as valuable and useful for enhancing intent-to-perform physical activity in individuals with SCI. The program has the potential to be applied in a variety of settings, but feasibility testing is required before implementing in a larger trial.
Role of Vitamin E for Nonalcoholic Steatohepatitis in Patients With Type 2 Diabetes: A Randomized Controlled Trial
Authors: Bril Fernando , Biernacki Diane M. , Kalavalapalli Srilaxmi , Lomonaco Romina , Subbarayan Sreevidya K. , Lai Jinping , Tio Fermin , Suman Amitabh , Orsak Beverly K. , Hecht Joan , Cusi Kenneth 
Published: July 2019

OBJECTIVE
While vitamin E has shown to improve nonalcoholic steatohepatitis (NASH) in patients without diabetes, information on patients with type 2 diabetes mellitus (T2DM) is lacking. The aim of this study was to determine whether vitamin E, alone or combined with pioglitazone, improves histology in patients with T2DM and NASH.

RESEARCH DESIGN AND METHODS
This was a proof-of-concept, randomized, double-blind, placebo-controlled trial conducted from 2010 to 2016. Patients with T2DM and biopsy-proven NASH (n = 105) were randomized to vitamin E 400 IU b.i.d., vitamin E 400 IU b.i.d. plus pioglitazone 45 mg/day, or placebo. Eighty-six patients completed the 18-month study. The primary end point was a two-point reduction in the nonalcoholic fatty liver disease activity score from two different parameters, without worsening of fibrosis. Secondary outcomes were resolution of NASH without worsening of fibrosis, individual histological scores, and metabolic parameters.

RESULTS
More patients on combination therapy achieved the primary outcome versus placebo (54% vs. 19%, P = 0.003) but not with vitamin E alone (31% vs. 19%, P = 0.26). Both groups showed improvements in resolution of NASH compared with placebo (combination group: 43% vs. 12%, P = 0.005; vitamin E alone: 33% vs. 12%, P = 0.04). While steatosis assessed by histology improved with combination therapy (P &lt; 0.001) and vitamin E alone (P = 0.018), inflammation (P = 0.018) and ballooning (P = 0.022) only improved with combination therapy. No improvement in fibrosis was observed in any group.

CONCLUSIONS
In this proof-of-concept study, combination therapy was better than placebo in improving liver histology in patients with NASH and T2DM. Vitamin E alone did not significantly change the primary histological outcome.
Relevant nursing measures for the adverse reactions associated with chimeric antigen receptor T cells (CAR-T) immunotherapy: a systematic review of case reports†
Authors: Zhang Xu , Sun Di , Jiang Gui-Chun 
Published: July 2019

Abstract

Objective
Cytokine release syndrome (CRS) and tumor lysis syndrome (TLS) that occur after chimeric antigen receptor T (CAR-T) cells are reinfused, which severely affect the survival and prognosis of patients. Although several articles have reported on the care of CAR-T cell immunotherapy, the quality of the study and the effectiveness of holistic nursing interventions have not been systematically reviewed. The purpose of this study was to systematically evaluate the existing holistic nursing interventions of CAR-T cell immunotherapy.

Methods
A literature search for keywords was performed in PubMed, EMBASE, the Cochrane Library, CNKI, CBM, and Wanfang Data from its inception until January 2018. Studies were deemed eligible if they comprised patients with tumor receiving CAR-T cell immunotherapy, described the holistic nursing process, and were published in Chinese and English.

Results
A total of 6 articles on holistic nursing interventions of CAR-T cell immunotherapy are reported, and the nursing methods and results of each article are analyzed. The quality of the studies included was medium. All nursing measures were considered effective.

Conclusions
Holistic nursing programs reduce the incidence of CRS and TLS and improve the quality of life of cancer patients.