bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modeling and Analysis of Mixed Traffic Networks with Human-Driven and Autonomous Vehicles
Authors: Xu Qing , Chen Chaoyi , Chang Xueyang , Cao Dongpu , Cai Mengchi , Wang Jiawei , Li Keqiang , Wang Jianqiang 
Published: November 2024

AbstractThe emergence of connected and automated vehicles (CAV) indicates improved traffic mobility in future traffic transportation systems. This study addresses the research gap in macroscopic traffic modeling of mixed traffic networks where CAV and human-driven vehicles coexist. CAV behavior is explicitly included in the proposed traffic network model, and the vehicle number non-conservation problem is overcome by describing the approaching and departure vehicle number in discrete time. The proposed model is verified in typical CAV cooperation scenarios. The performance of CAV coordination is analyzed in road, intersection and network scenario. Total travel time of the vehicles in the network is proved to be reduced when coordination is applied. Simulation results validate the accuracy of the proposed model and the effectiveness of the proposed algorithm.
Research on Influence Mechanism of Nozzle Structure on Properties of Thick Carbon Steel Plate by Laser Cutting
Authors: Chen Nan , Shi Yan , Pang Yan , Li Ruotao 
Published: November 2024

AbstractAiming at problems such as slow cutting speed, poor cutting quality and inability to cut thick plates effectively with traditional single-layer nozzle structure, it is proposed to add an outer ring core to form a double-layer nozzle structure on the basis of the single-layer nozzle structure to improve its cutting performance. The three-dimensional entity of single- and double-layer nozzle is established, and the simulation model of auxiliary gas flow field is established by using N-S equation and SST k-ω turbulence model assisted by laser cutting in this paper. The simulation results show that under the same cutting parameters, the auxiliary gas flow rate, oxygen concentration, mass flow rate, shear force and dynamic pressure of the double-layer nozzle structure are higher than those of the single-layer nozzle structure. In addition, compared with the single-layer nozzle structure, the double-layer nozzle structure can effectively improve the attenuation of oxygen concentration in the direction of sheet thickness. Two nozzle structures were used to laser cutting carbon steel plates with a thickness of 20 mm in the experiment, and the experimental parameters of cutting speed were 1.2–1.6 m/min. The comparison results show that the cutting speed and cutting section quality of the double-layer nozzle are better than those of the single-layer nozzle, and the results of simulation analysis are consistent with the cutting test results.
Sodium hypochlorite–based binderless technology for solid wood adhesion: application of a commercial bleaching agent for adhesion and observation of surface characteristics
Authors: Nakamura Kamii , Furuta Naoyuki , Miyazaki Junko , Nakamura Noboru 
Published: November 2024

AbstractIn this study, several methods for a novel binderless technology were explored, the effects of treatment conditions on the adhesive performance were examined and the surface was characterized during adhesion. The method entailed a three-step process for bonding solid wood, which involved immersion in sodium hypochlorite–containing treatment solutions, surface scratching, and drying with pressing. Various treatment conditions were investigated, including solution types, immersion durations, scratch treatment materials, and pressing methods. The resulting shear strength was 9.7 MPa, which is well above the Japanese Agricultural Standards value for laminated wood (6 MPa). In this method, the specimens were immersed in a bleach solution containing a surfactant for 16 h, followed by pressurization at 1 MPa for 24 h at 60 ℃ using a pressure-controlled press. Around the bonding area in the specimens, the cells in the immersed area were significantly compressed, and the aldehyde groups of the coniferyl aldehyde in the lignin were denatured or lost. Based on these findings, a new binderless technology was developed for solid wood without a high-temperature, high-pressure pressing step.
Survival cost sharing among altruistic full siblings in Mendelian population
Authors: Garay József , López Inmaculada , Varga Zoltán , Csiszár Villő , Móri Tamás F. 
Published: November 2024

Abstract
Background
We focus on Haldane’s familial selection in monogamous families in a diploid population, where the survival probability of each sibling is determined by altruistic food sharing with its siblings during starvation. An autosomal recessive-dominant or intermediate allele pair uniquely determines the altruistic or selfish behavior, which are coded by homozygotes. We focus on the case when additive cost and benefit functions determine the survival probability of each full sibling.

Results
We provide conditions for the existence of the altruistic and selfish homozygote. We show that the condition of evolutionary stability of altruism depends on the genotype-phenotype mapping. Furthermore, if the offspring size increases then the condition of evolutionary stability of altruism becomes stricter. Contrary to that, for the evolutionary stability of selfish behavior it is enough if the classical Hamilton’s rule does not hold. Moreover, when the classical Hamilton’s rule holds and the condition of evolutionary stability of altruism does not hold, then the selfish and altruistic phenotypes coexist.

Conclusions
In summary, the classical Hamilton’s rule is a sufficient condition for the existence of altruism, but it alone does not imply the evolutionary stability of the pure altruistic homozygote population when the altruistic siblings share the cost of altruism.
Clinical laboratory shadowing- an elective program in undergraduate health professions training: perception, strengths and challenges
Authors: Nayak Vineetha K. Ramdas , Shetty Prajna P. , Balamurugan Vaideki , Belle Vijetha Shenoy , Maradi Ravindra , Nayak Kirtana R. 
Published: November 2024

Abstract
Background
The authors aim to evaluate the perception, strengths, and challenges in the implementation of “Clinical Laboratory Shadowing” as an elective module for third-year professional medical students.

Methods
Clinical Laboratory shadowing is an elective module designed by the faculty of Biochemistry for Phase III part-2 medical students. The elective comprises modules explaining the functioning of the Biochemistry Laboratory in a hospital. The students are provided insights into the different processes involved in laboratory testing. They are taught about total quality management of the Biochemistry Laboratory. Thirteen students from Batch 2019 and eight students from Batch 2020 volunteered to attend the elective. Students’ feedback regarding the learning was analyzed with the help of a questionnaire. Pre-test and post-test questions were evaluated to test the knowledge gained during the elective. Two focus group discussions were conducted regarding the benefits and areas for improvement for the elective.

Results
Following the elective module there was a significant improvement in the test score compared to baseline. A majority (84–95%) of students perceived that the shadowing elective was well planned, and materials were well prepared, there was appropriate engagement with the facilitators, gained knowledge on the principles of total quality management, good laboratory practices, disease process, and diagnosis. Most (53–74%) of the students felt that this elective was not monotonous and not difficult. Around 84–95% of students felt that this elective module enhanced the importance of interprofessional communication and teamwork. Moreover, through focused group discussions, several recurring themes emerged. Among these, the scope for improvement within the module is profound. The faculties were of the opinion that the shift from conventional training to competency-based learning was effectively embraced by the students, underscoring the importance of consistent small group activities in the module to enhance the skills of Good laboratory practices. The students expressed a desire for additional clinical correlation and interpretation classes in the elective module, showing their preference for student-centred clinically oriented learning.

Conclusion
The current study concludes that including an elective module on Clinical laboratory shadowing is an overall positive experience for medical students in enhancing their knowledge of basic sciences and application of the concepts in diagnosing cases. This elective may be desirable to adopt in other medical colleges in India.
Cleavage and in vitro cultivation rates monitoring in culture media supplemented with energy sources, non-essential amino acids, and antioxidants in the buffalo embryos
Authors: Abu El-Naga Eman M , Ali Montaser E. , Ali Rawda H. , Hozyen Heba F. , Hussein Hassan A. 
Published: November 2024

AbstractThe study was designed to monitor the cleavage rate (CR) and in-vitro cultivation rate (IVC) after addition of energy sources, non-essential amino acids, and antioxidants to the Synthetic oviductal fluid (SOF) and FertiCult. After in-vitro maturation and in-vitro fertilization, presumptive zygotes were cultured in one of two culture media: FertiCult media and SOF medium, supplemented with pyruvate, glucose, and sodium lactate as energy sources, as well as 10, 20, 250, 500, and 750 mg non-essential amino acids, and antioxidants. All stages of cleavage rate (CR), and in-vitro cultivation rate (IVC) of embryonic development including morula stage (MOR) and blastocyst (BLAS) have been assessed. The findings revealed that there were no significant differences in the CR between the control and other treated groups with sources of energy when added to SOF media (P > 0.05), while there were significant differences (P < 0.05) in the IVC of embryonic development between groups (The percentages of MOR stage in the control, pyruvate, glucose and mixture of source of energy (MIX) were at 50%, 62.5%, 60%, and 63.6%, respectively). The highest percentage of the BLAS was recorded after SOF supplementation with glucose (40%). Similarly, there were no significant differences (P > 0.05) in the CR between control and FertiCult supplemented with sources of energy, while the IVC stages increased significantly (P < 0.05) in the FertiCult media supplemented with glucose, pyruvate, sodium lactate, and MIX. The percentages of the MOR stage in the control, pyruvate, glucose and mix media were at 50%, 55.6%, 55.6%, 54.5%, 57.1% respectively. The lowest percentage of the BLAS was recorded after FertiCult supplementation with pyruvate (11.1%). Replenishing the SOF maturation media with 20 mg of non-essential amino acids significantly (P < 0.05) enhanced the MOR stage (100%). There was also an improvement in the development of BLAS stage, where it reached 31.2% and 47.4% in the SOF maturation media supplemented with 10, and 750 mg non-essential amino acids, respectively. There were no significant differences (P > 0.05) in neither CR nor IVC between control and FertiCult supplemented with antioxidants. There were significant differences (P < 0.05) in the MOR stages (control, 42.9% & treated, 57.9%) and BLAS stages (control, 21.4% & treated, 42.1%) in antioxidant supplemented SOF maturation media compared to control. In conclusion, supplementation of SOF cultivation medium with energy sources, 20 mg of non-essential amino acids and antioxidant addition may improve the cleavage rate (CR) and in vitro cultivation rate (IVC) of buffalos’ embryonic development.
Temperature and feeding frequency: interactions with growth, immune response, and water quality in juvenile Nile tilapia
Authors: Hamed Sara , El-Kassas Seham , Abo-Al-Ela Haitham G. , Abdo Safaa E. , Abou-Ismail Usama A. , Mohamed Radi A. 
Published: November 2024

Abstract
Background
Water temperature and feeding frequency are critical abiotic factors regulating the growth and immune function of aquatic organisms. This study investigated the effects of water temperature and feeding frequency on growth and immune function in Nile tilapia (Oreochromis niloticus) over two months. A total of 360 juvenile fish (average weight: 20.00 ± 1.26 g) were divided into six groups, each with three replicates, based on a combination of three water temperatures (26, 28, and 30 °C) and two feeding frequencies (either 1 or 2 meals per day).

Results
At 30 ºC and 28 ºC, water electrical conductivity and total dissolved salts increased, while total ammonia nitrogen and dissolved oxygen rose slightly in groups fed twice daily, with a significant interaction between temperature and feeding frequency. The group at 30 ºC with two meals per day showed the highest final body weight (FBW). The interaction between temperature and feeding frequency significantly influenced FBW, total feed intake, and body thickness. Fish at 30 ºC exhibited upregulated hepatic growth hormone receptor 1 and insulin-like growth factor 1, while those at 28 ºC with one meal per day, as well as those at 30 ºC regardless of meal frequency, also showed increased expression of hepatic fatty acid binding protein and intestinal cluster of differentiation 36. Fish at 30 ºC had upregulated leptin levels and downregulated cholecystokinin, while those at 26 ºC displayed the opposite trend, particularly with one meal daily. Higher temperatures significantly boosted serum IgM, superoxide dismutase (SOD), and lysozyme (LYZ) levels, with meal frequency also affecting malondialdehyde, IgM, and SOD levels. Additionally, 30 ºC enhanced the hepatic expression of mucin-like protein (muc), oligo-peptide transporter 1 (pept1), interleukin 1, nf-κB, complement C3, lyz, sod, catalase, and glutathione peroxidase, with twice-daily meals having a more pronounced effect. Conversely, 28 ºC with one meal per day upregulated some of these genes, such as muc, pept1, and sod.

Conclusions
Overall, 30 ºC with two meals per day significantly improved the growth and health of juvenile Nile tilapia, while 28 ºC with two meals maintained satisfactory performance.
Factors predicting composite grafts survivability in patients with fingertip amputations; a systematic review and meta-analysis
Authors: Elameen Ali Mohamed , Dahy Asmaa Ali , Abu-Elsoud Ahmed , Gad Amany Attalah 
Published: November 2024

Abstract
Background
Fingertip amputation is a commonly encountered injury in emergency settings. Composite grafting is a non-microsurgical alternative maintaining digit length with no donor site morbidities. This meta-analysis was conducted to retrieve factors associated with composite graft survivability among patients with fingertip amputations.

Methods
A literature review throughout twelve databases was performed on 24 July 2023. All clinical studies comparing the patients-related, trauma-related, or amputation-related variables among patients with survived and non-survived composite grafting were eligible for meta-analysis. Single-arm studies reported the potential predictors of composite graft survival among patients with fingertip injuries treated with composite grafting were included.

Results
This review included ten articles with 720 fingertips composite grafting. Of them, 526 grafts survived, with a pooled overall survivability of 72.8%. There was a significant association between younger age (OR 2.31,95%CI 1.10, 4.87, P = 0.03), level of amputation (I) (OR 0.31,95% CI 0.14 to 0.67, P = 0.003), and successful composite grafting. There was no statistically significant (P = 0.449) impact of time to composite grafting on the likelihood of composite graft survivability.

Conclusion
Composite grafting is a feasible and effective procedure for restoring aesthetically functional digits among patients with traumatically amputated fingertips. The composite graft survived among the majority of the patients, with a more significant survival pattern among younger populations and patients with more distal amputations.

Level of evidence
Level III.
AQbD-enhanced green RP-UPLC-PDA methodology for quantification and forced degradation studies for omeprazole, amoxicillin, and rifabutin
Authors: Baffinsha S. P. Ashnah , Ragupathi Vijayageetha , Chanduluru Hemanth Kumar 
Published: November 2024

AbstractThe ternary combination like omeprazole (OMP), amoxicillin (AMX), and rifabutin (RFB) was approved by the FDA in November 2019 for combating Helicobacter pylori infections and ulcers caused by this infection. This study aims to develop and authenticate a robust and eco-friendly RP-UPLC technique aimed at the concurrent analysis of OMP, AMX, and RFB, following ICH guidelines, Analytical Quality by Design (AQbD), and green analytical chemistry (GAC) principles. The analysis used the Thermo C18 column (100 mm × 2.1 mm, 1.7 µm), ethanol, and formic acid solution (43:57) as mobile phase with a flow rate of 0.2 ml/min at 272 nm. The method was developed based on the ICH Q14 and validated according to ICH Q2(R1) followed by Forced degradation studies under various conditions. The method showed good linearity for OMP, AMX, and RFB, with coefficient of determination (r2) of 0.9995, 0.9993, and 0.9997, respectively. Precision studies indicated low %RSD values, confirming high reproducibility. Forced degradation studies confirmed the stability of the drugs for 30 min in acid, base, and redox reactions, and they were also stable for 6 h at 105 °C in dry conditions. GAPI assessment depicted a green and yellow pictogram, AGREE scored 0.85, BAGI scored 80, and RGB12 Whiteness Assessment Tool scored 97.5%. The developed RP-UPLC-PDA technique is robust and reliable for the concurrent quantification of the triple combination. It aligns with sustainability goals, enhancing the efficiency and environmental sustainability of pharmaceutical analysis, and setting a benchmark for future analytical methods.
Novel insights into antioxidant status, gene expression, and immunohistochemistry in an animal model infected with camel-derived Trypanosoma evansi and Theileria annulata
Authors: Ramadan Reem M. , Bakr Alaa F. , Fouad Esraa , Mohammed Faten F. , Abdel-Wahab Azza M. , Abdel-Maogood Sahar Z. , El-Bahy Mohamed M. , Salem Mai A. 
Published: November 2024

Abstract
Background
Hemoprotozoan diseases, especially trypanosomosis and theileriosis, adversely affect the productivity, growth, and performance of camels. Regular sampling and investigation of camels are challenging due to several factors. Consequently, there is a lack of knowledge on camel parasite genotyping, cytokine production, and oxidative stress parameters during infection.

Methods
The present study investigated two critical blood protozoa infecting camels in Egypt, Trypanosoma evansi and Theileria annulata, using molecular methods, specifically 18S rRNA gene analysis. Following molecular confirmation, experimental infections were induced in Swiss albino mice to assess the expression of immune response genes and oxidative stress parameters. The study further explored the correlation between histopathological alterations and inflammatory reactions in the kidney, spleen, and liver of infected mice, alongside the immunohistochemical expression of caspase-3, proliferating cell nuclear antigen (PCNA), and tumor necrosis factor (TNF).

Results
Trypanosoma evansi and T. annulata isolated from naturally infected camels were molecularly identified and deposited in GenBank under accession numbers OR116429 and OR103130, respectively. Infection with T. evansi and T. annulata caused significant adverse effects on the immune condition of infected mice, increasing the pathogenicity of the infection. This was evidenced by a significant increase in oxidative stress parameter levels in both naturally infected camels and experimentally infected mice compared to healthy controls. Furthermore, the expression of immune response genes was significantly elevated in infected mice. Immunohistochemistry analysis showed a pronounced upregulation of caspase-3, PCNA, and TNF in the infected groups relative to the control group. These findings are the first to be reported in Egypt.

Conclusions
This study successfully identified and genotyped two economically important blood protozoa, T. evansi and T. annulata, from camels in Egypt. Additionally, the experimental animal model provided valuable insights into the immune response, oxidative stress, and histopathological changes induced by these parasites, demonstrating comparable results to naturally infected camels. These findings highlight the potential of this model to study parasite–host interactions and immune responses, contributing to a better understanding of the pathogenic mechanisms of T. evansi and T. annulata infections. This model may be useful for future studies focused on disease control and therapeutic interventions.

Graphical Abstract
The role of agricultural cooperatives in mitigating opportunism in the context of complying with sustainability requirements: empirical evidence from Spain
Authors: Sánchez-Navarro Jorge Luis , Arcas-Lario Narciso , Bijman Jos , Hernández-Espallardo Miguel 
Published: November 2024

AbstractThe convergence of emerging sustainability regulations and recommendations outlined in the European, national and regional agricultural policies, coupled with the growing demand from retailers for food produced through more sustainable agriculture practices, presents a substantial challenge for farmers. This challenge is further exacerbated by their limited access to essential information, knowledge, and resources necessary for compliance, which are often acquired through interactions with various stakeholders within the agri-food supply chain. Moreover, the inherent power asymmetry between small-scale farmers and their considerably larger counterparts, including input suppliers and agricultural product buyers, exposes farmers to opportunistic behaviours. In response to these challenges, agri-food cooperatives have been proposed as an organizational solution to mitigate opportunistic behaviour. However, empirical data-supported evidence of this proposition remains scarce. Drawing upon data obtained from Spanish farmers, our study investigates the impact of agri-food cooperatives on the incidence of opportunistic practices experienced by farmers during their interactions with suppliers and buyers. Through a propensity score matching analysis, our findings reveal that cooperative membership exerts a statistically significant negative influence on both supplier and buyer opportunism in the context of complying with sustainability requirements. These findings provide compelling empirical evidence of the pivotal role played by agri-food cooperatives in addressing opportunism within the supply chain. Importantly, they underscore the vital importance of cooperatives in mitigating the challenges associated with enhancing sustainability in agriculture.