bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing patient-centred chiropractic care in Canada: identifying barriers, enablers, and strategies through a qualitative needs assessment
Authors: To Daphne , Southerst Danielle , Atkinson-Graham Melissa , Yu Hainan , Connell Gaelan , Draper Crystal , Cancelliere Carol 
Published: November 2024

Abstract
Background
The Canadian Chiropractic Association (CCA) initiated a quality improvement project to develop best practices aimed at enhancing the patient experience.

Objectives
(1) Identify and prioritise the key moments in the new patient experience that could be improved by providing chiropractors with focused support and resources; (2) explore views, barriers, and enablers to implementing these best practices; and (3) develop recommendations to facilitate the adoption of these practices.

Methods
We conducted a qualitative needs assessment using a human-centred design approach, focused on understanding the needs and experiences of end-users to create tailored solutions. The Theoretical Domains Framework (TDF) was employed to explore chiropractors’ knowledge use and behaviour change, and TDF domains were mapped to Behaviour Change Techniques (BCTs) to develop targeted strategies for addressing identified barriers and enablers. Thirteen chiropractors from across Canada participated in semi-structured interviews and related activities.

Results
The key moments where participants felt they needed the most support were “treatment”, “report of findings”, “informed consent”, “physical examination”, and “before the appointment”. All participants agreed with the best practices seed statements. Key barriers included gaps in knowledge, communication skills, and resource availability, particularly in rural areas. Enablers included collaboration with other health professionals, mentorship, and access to practice tools. Recommendations include enhanced training in communication and treatment planning, increased access to resources in rural areas, and fostering collaborative relationships among health professionals.

Conclusion
Understanding the barriers and enablers to implementing best practices can inform targeted strategies to improve patient-centred care in chiropractic practice across Canada.
Genetic-by-age interaction analyses on complex traits in UK Biobank and their potential to identify effects on longitudinal trait change
Authors: Winkler Thomas W. , Wiegrebe Simon , Herold Janina M. , Stark Klaus J. , Küchenhoff Helmut , Heid Iris M. 
Published: November 2024

Abstract
Background
Genome-wide association studies (GWAS) have identified thousands of loci for disease-related human traits in cross-sectional data. However, the impact of age on genetic effects is underacknowledged. Also, identifying genetic effects on longitudinal trait change has been hampered by small sample sizes for longitudinal data. Such effects on deteriorating trait levels over time or disease progression can be clinically relevant.

Results
Under certain assumptions, we demonstrate analytically that genetic-by-age interaction observed in cross-sectional data can be indicative of genetic association on longitudinal trait change. We propose a 2-stage approach with genome-wide pre-screening for genetic-by-age interaction in cross-sectional data and testing identified variants for longitudinal change in independent longitudinal data. Within UK Biobank cross-sectional data, we analyze 8 complex traits (up to 370,000 individuals). We identify 44 genetic-by-age interactions (7 loci for obesity traits, 26 for pulse pressure, few to none for lipids). Our cross-trait view reveals trait-specificity regarding the proportion of loci with age-modulated effects, which is particularly high for pulse pressure. Testing the 44 variants in longitudinal data (up to 50,000 individuals), we observe significant effects on change for obesity traits (near APOE, TMEM18, TFAP2B) and pulse pressure (near FBN1, IGFBP3; known for implication in arterial stiffness processes).

Conclusions
We provide analytical and empirical evidence that cross-sectional genetic-by-age interaction can help pinpoint longitudinal-change effects, when cross-sectional data surpasses longitudinal sample size. Our findings shed light on the distinction between traits that are impacted by age-dependent genetic effects and those that are not.
Multi-omics landscape of childhood simple obesity: novel insights into pathogenesis and biomarkers discovery
Authors: Ren Yi , Huang Peng , Zhang Lu , Tang Yufen , He Siyi , Li HaiDan , Huang XiaoYan , Ding Yan , Liu Lingjuan , Liu Liqun , He Xiaojie 
Published: November 2024

Abstract
Background
The increasing incidence of childhood obesity annually has led to a surge in physical and mental health risks, making it a significant global public health concern. This study aimed to discover novel biomarkers of childhood simple obesity through integrative multi-omics analysis, uncovering their potential connections and providing fresh research directions for the complex pathogenesis and treatment strategies.

Methods
Transcriptome, untargeted metabolome, and 16 S rDNA sequencing were conducted on subjects to examine transcripts, metabolites in blood, and gut microflora in stool.

Results
Transcriptomic analysis identified 599 differentially expressed genes (DEGs), of which 25 were immune-related genes, and participated in immune pathways such as antimicrobial peptides, neutrophil degranulation, and interferons. The optimal random forest model based on these genes exhibited an AUC of 0.844. The metabolomic analysis examined 71 differentially expressed metabolites (DEMs), including 12 immune-related metabolites. Notably, lauric acid showed an extremely strong positive correlation with BMI and showed a good discriminative power for obesity (AUC = 0.82). DEMs were found to be significantly enriched in four metabolic pathways, namely “Aminoacyl-tRNA biosynthesis”, “Valine leucine and isoleucine biosynthesis, and Glycine”, “Serine and threonine metabolism”, and “Biosynthesis of unsaturated fatty acids”. Microbiome analysis revealed 12 differential gut microbiotas (DGMs) at the phylum and genus levels, with p_Firmicutes dominating in the obese group and g_Escherichia-Shigella in the normal group. Subsequently, a Random Forest model was developed based on the DEMs, immune-related DEGs, and metabolites with an AUC value of 0.912. The 14 indicators identified by this model could potentially serve as a set of biomarkers for obesity. The analysis of the inter-omics correlation network found 233 pairs of significant correlations. DEGs BPIFA1, BPI, and SAA1, DEMs Dimethy(tetradecyl)amine, Deoxycholic acid, Pathalic anhydride, and DL-Alanine, and DGMs g_Intestinimonas and g_Turicibacter showed strong connectivity within the network, constituting a large proportion of interactions.

Conclusion
This study presents the first comprehensive description of the multi-omics characteristics of childhood simple obesity, recognizing promising biomarkers. Immune-related markers offer a new perspective for researching the immunological mechanisms underlying obesity and its associated complications. The revealed interactions among these biomarkers contribute to a deeper understanding the intricate biological regulatory networks associated with obesity.
Aggregation of cardiovascular risk factors in a cohort of 40-year-olds participating in a population-based health screening program in Sweden
Authors: Borgström Bolmsjö Beata , Stenman Emelie , Grundberg Anton , Sundquist Kristina 
Published: November 2024

Abstract
Background
It is important to identify and evaluate cardiovascular risk factors at an early stage to address them accordingly. Among the younger population, the metabolic syndrome is less common than in older ages. However, each separate metabolic risk factor still has an additive effect on cardiovascular risk factor burden. Non-metabolic risk factors that occur in the younger population include family history, smoking, psychological distress and socioeconomic vulnerability. In 2021 a voluntary health intervention program was introduced in an urban area in Sweden where a cohort of 40-year-olds was invited for cardiovascular risk identification. The aim of this study was to identify how cardiovascular risk factors tend to aggregate in individuals participating in a voluntary health screening program and how the metabolic risk factors associate with non-metabolic cardiovascular risk factors.

Methods
This was a cross-sectional study with 1831 participants. Data from questionnaires and baseline measurements were used to calculate the prevalence of metabolic- (blood pressure, lipids, fasting plasma glucose, BMI, waist-hip ratio) and non-metabolic risk factors (family history of CVD, smoking, psychological distress, socioeconomic vulnerability) for CVD. SCORE2 was calculated according to the algorithm provided by the SCORE2 working group and ESC (European Society of Cardiology) Cardiovascular Risk Collaboration. Associations among each of the metabolic risk factors and non-metabolic risk factors were estimated using logistic regression and presented as odds ratios (ORs) with 95% confidence intervals (CIs).

Results
More than half of the study population had at least one metabolic risk factor, and more than 1/3 was considered to be suffering from psychological distress. Furthermore, obesity or central obesity demonstrated individual associations with all of the non-metabolic risk factors in the study; smoking (1.49; 1.32–2.63), family history of CVD (1.41; 1.14–1.73), socioeconomic vulnerability (1.60; 1.24–2.07), and psychological distress (1.40; 1.14–1.72). According to SCORE2 25% of the men were at moderate risk (2.5–7.5%) of developing a cardiovascular event within 5–10 years, but only 2% of the women.

Conclusions
Obesity/central obesity should be a prioritized target in health screening programs. The non-metabolic risk factors, socioeconomic vulnerability, and psychological distress should not be ignored and addressed with adequate guidance to create health equity.
Reconstructive surgical treatment of peri-implantitis with use of a chitosan brush for decontamination- case series with 1-year follow-up
Authors: İnce Kuka Gizem , Gürsoy Hare 
Published: November 2024

Abstract
Introduction and aim
There is a need for studies assessing the efficacy of different therapeutic approaches in the reconstructive surgical treatment of peri-implantitis. The aim of this case series is to evaluate the clinical outcomes and radiographic bone fill of reconstructive surgical treatment using the oscilating chitosan brush for implant surface decontamination.

Materials and methods
Nine patients with 11 Class I and III peri-implantitis defects were included. Following implant surface decontamination performed with a chitosan brush, guided bone regeneration (GBR) was performed by means of a bovine derived cancellous bone graft and collagen membrane. Clinical parameters such as full mouth plaque score (FMPS), probing depth (PD), bleeding on probing (BoP), recession, and radiographic bone level (RBL) were recorded at baseline and 1 year following treatment.

Results
All evaluated parameters, including PD, BoP, recession, RBL, and full mouth plaque scores, revealed significant improvements at 1 year follow-up compared to baseline (p < 0.05). The mean PD values reduced from 7.30 ± 1.29 to 3.78 ± 0.65 (p = 0.000). RBL was detected 5.50 ± 1.31 and 1.38 ± 0.74 at baseline and 1-year, respectively (p = 0.010).

Conclusion
Reconstructive surgical treatment of Class I and III peri-implantitis defects with GBR may be an effective treatment protocol when an oscilitating chitosan brush is used for surface decontamination.

Clinical trial number
Not applicable.
Serous endometrial carcinoma metastatic to the sigmoid colon masquerading as a primary colon cancer detected by bowel obstruction
Authors: Shimura Taiga , Fukushima Naoko , Tsuboi Kazuto , Suzuki Toshimasa , Kajimoto Tetsuya 
Published: November 2024

Abstract
Background
The majority of colorectal malignancies are primary tumors. Secondary tumors are rare, and colorectal metastasis from endometrial carcinoma is exceptionally uncommon. We report a case of serous endometrial carcinoma that metastasized to the sigmoid colon, initially presenting as a primary colon carcinoma due to bowel obstruction.

Case presentation
A 79-year-old woman presented with abdominal distention and constipation. Five years earlier, she had undergone an open total hysterectomy with bilateral salpingo-oophorectomy for endometrial serous carcinoma. A colonoscopy revealed stenosis encircling the entire sigmoid colon. Abdominal computed tomography demonstrated irregular wall thickening in the sigmoid colon with enhanced regional lymph node enlargement and upstream bowel dilatation. Peritoneal dissemination in the pelvis was also noted. To relieve the obstruction, two self-expanding metal stents were inserted. Subsequently, due to the presumed diagnosis of sigmoid colon carcinoma with peritoneal dissemination, an open left hemicolectomy with resection of the peritoneal dissemination was performed. Histopathological examination identified the colon tumor and peritoneal dissemination as metastatic serous endometrial carcinoma. Immunohistochemical studies showed the tumor cells were negative for CK7, CK20, and CDX2. No chemotherapy was administered, and 6 months post-operation, no recurrence was observed.

Conclusions
Metastasis of endometrial carcinoma to the colon is extremely rare. Diagnosing a colon tumor as a metastasis from endometrial carcinoma is challenging during preoperative examinations. Therefore, in patients with a history of endometrial carcinoma, the possibility that the primary site might be the uterus should be considered.
Effective use of a supraglottic airway (i-gel™) during emergence from anesthesia in a patient with multiple giant bullae
Authors: Arime Hayato , Asai Takashi , Fujishiro Asuka , Saito Tomoyuki 
Published: November 2024

Abstract

Background
Anesthetic management of a patient with multiple giant bullae is generally difficult due to an increased risk of respiratory complications, and there is no consensus regarding safe extubation methods. We report a case of an effective use of a supraglottic airway (i-gel™) during emergence from anesthesia in a patient with multiple giant bullae, in whom a double-lumen bronchial tube was being used during anesthesia.

Case presentation
A 52-year-old man with multiple giant bullae underwent video-assisted pulmonary resections, while the ventilation was controlled via a double-lumen bronchial tube. After successful thoracoscopic surgery, an i-gel™ was inserted while the double-lumen tube was still in place, and the double-lumen tube was subsequently removed under deep anesthesia. The i-gel™ was removed without complications after the patient had become able to respond to verbal command.

Conclusion
We believe that this method would minimize the risk of trauma to the respiratory system during emergence from anesthesia in patients with multiple giant bullae.
Development of a self-report measure of GPS uses and its relationship with environmental knowledge and self-efficacy and pleasure in exploring
Authors: Miola L. , Muffato V. , Boldrini A. , Pazzaglia F. , Meneghetti C. 
Published: November 2024

AbstractThe widely utilized Global Positioning System (GPS) plays a crucial role in everyday navigation. The literature has predominantly focused on GPS use for reaching destinations rather than exploring its various strategic applications and relations with individual factors. The current paper is intended to develop a GPS Uses Scale assessing a variety of GPS uses for wayfinding and other GPS uses (Study 1). We also examine whether GPS uses are related to gender, age, self-efficacy and pleasure in exploring, dependence on GPS devices, and environment knowledge (Study 2). In Study 1, 365 participants completed the new GPS Uses Scale and the McGill GPS questionnaire, for assessing validity. Results from the confirmatory factor analysis confirmed a structure as five-level factors, good reliability, and validity. In Study 2, 200 participants completed the GPS Uses Scale, self-efficacy and pleasure in exploring scale, GPS dependence scale, and a sketch map task after learning a virtual city from a video. Results from the linear model showed that those who use GPS for strategic purposes reported higher self-efficacy and pleasure in exploring as well as dependence on GPS. Moreover, those who use GPS for orientation purposes reported higher dependency on GPS and had higher scores on the map task (environment knowledge). Men were less likely to use GPS for orientation. The present paper outlines the importance of assessing the various uses of GPS, suggesting self-efficacy and dependence on GPS, and contributes to its strategic use.
Harnessing the impact of beneficial microorganisms to control Meloidogyne incognita in tomato cultivation across diverse environments
Authors: Swe Win Lai Lai , Tóth Ferenc , Petrikovszki Renáta , Ladányi Márta , Fail József 
Published: November 2024

Abstract
Background
Tomato (Solanum lycopersicum L.) can overcome productivity challenges caused by plant-parasitic nematodes, especially Meloidogyne incognita. M. incognita is the most harmful polyphagous endoparasitic nematode of various cultivated crops globally and causes huge yield losses. The use of traditional chemical nematicides poses environmental and health risks, which is why safe alternatives are being explored. This study aimed to find alternatives to peat as a potting medium and evaluate their effectiveness in enhancing plant resilience against nematodes. Specifically, the study assessed the efficacy of four biocontrol agents (Trichoderma asperellum, Beauveria bassiana, Fusarium proliferatum and Bacillus mojavensis) in peat-based and compost-based potting media under open-field and greenhouse conditions.

Result
The research analyzes their impact on plant growth performance and their capacity to mitigate the severity of galling, the primary symptom caused by M. incognita in tomato cultivation. The results showed insignificant difference between peat and compost-based media in open-field (F(1,101) = 0.001, p = 0.97) and greenhouse (F(1,90) = 2.53, p = 0.12) experiments. However, biocontrol agents differed significantly in effectiveness (open field: F(4,101) = 5.85, p < 0.001; greenhouse: F(4,90) = 15.88, p < 0.001). B. mojavensis reduced the gall index by 81.24% in compost-based medium, and B. bassiana reduced it by 68.86% in peat-based medium.

Conclusion
This study unequivocally demonstrated the remarkable efficacy of F. proliferatum and T. asperellum in promoting aboveground plant development. The application of biocontrol agents F. proliferatum, T. asperellum, B. bassiana and B. mojavensis resulted in a substantial 25–81% reduction in nematode galling index compared to the untreated control.
Different metabolite profiles across Penicillium roqueforti populations associated with ecological niche specialisation and domestication
Authors: Crequer E. , Coton E. , Cueff G. , Cristiansen J. V. , Frisvad J. C. , Rodríguez de la Vega R. C. , Giraud T. , Jany J.-L. , Coton M. 
Published: November 2024

AbstractFungi are known to produce many chemically diversified metabolites, yet their ecological roles are not always fully understood. The blue cheese fungus Penicillium roqueforti thrives in different ecological niches and is known to produce a wide range of metabolites, including mycotoxins. Three P. roqueforti populations have been domesticated for cheese production and two populations thrive in other anthropized environments, i.e., food, lumber and silage. In this study, we looked for differences in targeted and untargeted metabolite production profiles between populations using HPLC-HR-Q-TOF and UHPLC-Q-TOF-HR-MS/MS. The non-cheese populations produced several fatty acids and different terpenoids, lacking in cheese strains. The Termignon cheese population displayed intermediate metabolite profiles between cheese and non-cheese populations, as previously shown for other traits. The non-Roquefort cheese population with the strongest domestication syndrome, produced the lowest quantities of measured metabolites, including mycophenolic acid (MPA), andrastin A and PR toxin. Its inability to produce MPA was due to a deletion in the mpaC gene, while a premature stop codon in ORF 11 of the PR toxin gene cluster explained PR toxin absence and the accumulation of its intermediates, i.e., eremofortins A and B. In the Roquefort population, we detected no PR toxin nor eremofortins A or B, but found no indel or frameshift mutation, suggesting downregulation. The hypotoxigenic trait of domesticated cheese populations can be hypothesized to be linked to the loss of this ability through trait degeneration and/or the selection of low toxin producers. It may also be due to the fact that populations from other anthropized environments maintained high metabolite diversity as the bioactivities of these compounds are likely important in these ecological niches.
The identification of key genes and pathways in polycystic ovary syndrome by bioinformatics analysis of next-generation sequencing data
Authors: Alur Varun , Vastrad Basavaraj , Raju Varshita , Vastrad Chanabasayya , Kotturshetti Shivakumar 
Published: November 2024

Abstract
Background
Polycystic ovary syndrome (PCOS) is a reproductive endocrine disorder. The specific molecular mechanism of PCOS remains unclear. The aim of this study was to apply a bioinformatics approach to reveal related pathways or genes involved in the development of PCOS.

Methods
The next-generation sequencing (NGS) dataset GSE199225 was downloaded from the gene expression omnibus (GEO) database and NGS dataset analyzed is obtained from in vitro culture of PCOS patients’ muscle cells and muscle cells of healthy lean control women. Differentially expressed gene (DEG) analysis was performed using DESeq2. The g:Profiler was utilized to analyze the gene ontology (GO) and REACTOME pathways of the differentially expressed genes. A protein–protein interaction (PPI) network was constructed and module analysis was performed using HiPPIE and cytoscape. The miRNA-hub gene regulatory network and TF-hub gene regulatory network were constructed. The hub genes were validated by using receiver operating characteristic (ROC) curve analysis.

Results
We have identified 957 DEG in total, including 478 upregulated genes and 479 downregulated gene. GO terms and REACTOME pathways illustrated that DEG were significantly enriched in regulation of molecular function, developmental process, interferon signaling and platelet activation, signaling, and aggregation. The top 5 upregulated hub genes including HSPA5, PLK1, RIN3, DBN1, and CCDC85B and top 5 downregulated hub genes including DISC1, AR, MTUS2, LYN, and TCF4 might be associated with PCOS. The hub gens of HSPA5 and KMT2A, together with corresponding predicted miRNAs (e.g., hsa-mir-34b-5p and hsa-mir-378a-5p), and HSPA5 and TCF4 together with corresponding predicted TF (e.g., RCOR3 and TEAD4) were found to be significantly correlated with PCOS.

Conclusions
These study uses of bioinformatics analysis of NGS data to obtain hub genes and key signaling pathways related to PCOS and its associated complications. Also provides novel ideas for finding biomarkers and treatment methods for PCOS and its associated complications.