bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential regulation and interaction of homoeologous WRKY18 and WRKY40 in Arabidopsis allotetraploids and biotic stress responses
Authors: Abeysinghe Jayami K. , Lam Kai‐Man , Ng Danny W.‐K. 
Published: October 2018

SummaryWRKY transcription factors (TFs) belong to a large family of regulatory proteins in plants that modulate many plant processes. Extensive studies have been conducted on WRKY‐mediated defense response in Arabidopsis thaliana and several crop species. Here, we aimed to investigate the potential roles and contributions of WRKY TFs in improving the defense response in the resynthesized Arabidopsis allotetraploids (Arabidopsis suecica) derived from two related autotetraploid progenitors, Arabidopsis thaliana (At4) and Arabidopsis arenosa (Aa). Rapid and differential induction of WRKY18 and WRKY40 expression was evident in response to Pseudomonas syringae and salicylic acid (SA) treatments in the allotetraploids. Selected direct targets of the WRKYs and PR1 also showed altered induction kinetics in the allotetraploids. Cleaved amplified polymorphic sequence analysis further revealed the accumulation of preferential homoeologous alleles (AtWRKY18, AaWRKY40, and AtWRKY60) in the allotetraploids, suggesting the potential for altered protein–protein interaction networks in the hybrids. Indeed, results showed that the cis‐interacting AtWRKY18/AtWRKY18 homodimer or trans‐interacting AtWRKY18/AaWRKY40 heterodimer exists as the preferred dimer interaction. Moreover, differential affinities of WRKY18 and WRKY40 homo‐ and heterodimers toward the W‐boxes in the WRKY60 promoter were observed. Transient and stable expression of the selected WRKYs in transgenic Arabidopsis further supported the idea that differential interactions lead to changes in PR1 induction and direct target expression under stress, respectively. Our data suggest that differential expression as well as differences in the strength of protein–protein and/or protein–DNA interactions among the WRKY homoeologs could lead to altered regulatory networks of defense genes, contributing to improved defense in allotetraploids.
The arginine decarboxylase gene ADC1, associated to the putrescine pathway, plays an important role in potato cold‐acclimated freezing tolerance as revealed by transcriptome and metabolome analyses
Authors: Kou Shuang , Chen Lin , Tu Wei , Scossa Federico , Wang Yamei , Liu Jun , Fernie Alisdair R. , Song Botao , Xie Conghua 
Published: October 2018

SummaryLow temperature severely influences potato production as the cultivated potato (Solanum tuberosum) is frost sensitive, however the mechanism underlying the freezing tolerance of the potato is largely unknown. In the present research, we studied the transcriptome and metabolome of the freezing‐tolerant wild species Solanum acaule (Aca) and freezing‐sensitive cultivated S. tuberosum (Tub) to identify the main pathways and important factors related to freezing tolerance. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation indicated that polyamine and amino acid metabolic pathways were specifically upregulated in Aca under cold treatment. The transcriptome changes detected in Aca were accompanied by the specific accumulation of putrescine, saccharides, amino acids and other metabolites. The combination of transcriptome and metabolome analyses revealed that putrescine exhibited an accumulative pattern in accordance with the expression of the arginine decarboxylase gene ADC1. The primary role of putrescine was further confirmed by analyzing all three polyamines (putrescine, spermidine, and spermine) and the genes encoding the corresponding enzymes in two sets of potato genotypes with distinct freezing tolerance, implying that only putrescine and ADC1 were uniquely enhanced by cold in the freezing‐tolerant genotypes. The function of putrescine was further analyzed by its exogenous application and the overexpression of SaADC1 in S. tuberosum cv. E3, indicating its important role(s) in cold‐acclimated freezing tolerance, which was accompanied with the activation of C‐repeat binding factor genes (CBFs). The present research has identified that the ADC1‐associated putrescine pathway plays an important role in cold‐acclimated freezing tolerance of potato, probably by enhancing the expression of CBF genes.
Methods for Activity Monitor Validation Studies: An Example With the Fitbit Charge
Authors: DeShaw Kathryn J. , Ellingson Laura , Bai Yang , Lansing Jeni , Perez Maria , Welk Greg 
Published: October 2018

Purpose: To advance research practices with consumer monitors, standard validation methods are needed. This study provides an example of best practices through systematically evaluating the validity of the Fitbit Charge (FBC) under free-living conditions using a strong reference measure and robust measurement agreement methods. Methods: 94 healthy participants (Mage 41.8 ±9.3 yrs) wore a FBC and two research grade accelerometers (Actigraph GT3X and activPAL) as they went about normal activities for a week. Estimated daily minutes of moderate to vigorous physical activity (MVPA) from the FBC were compared against reference estimates obtained from the Sojourns Including Posture (SIP) methodology, while daily step counts were compared against the activPAL. Results: Correlations with reference indicators were high for average daily MVPA (r = 0.8; p < .0001) and steps (r = 0.76; p < .0001), but the FBC overestimated time spent in MVPA by 56% and steps by 15%. The mean absolute percent errors of MVPA and steps estimated by FBC were 71.5% and 30.0%, respectively. Neither of the MVPA and step estimates from the FBC fell into the ±10% equivalence zone set by the criterion. The Kappa statistics of the classification agreement between the two MVPA assessment methods was 0.32 with a low sensitivity of 30.1% but a high specificity of 96.7%. Conclusion: The FBC overestimated minutes of MVPA and steps when compared to both reference assessments in free-living conditions. Standardized reporting in future studies will facilitate comparisons with other monitors and with future versions of the FBC.
Stationary Exercise in Overweight and Normal Weight Children
Authors: Yaghoubi Mostafa , Fink Philip W. , Page Wyatt H. , Shultz Sarah P. 
Published: October 2018

Purpose: This study examined differences in lower extremity kinematics and muscle activation patterns between normal weight (NW) and overweight (OW) children during stationary exercises (running in place, frontal kick, and butt kick) at submaximal intensity. Methods: Healthy children (aged 10–13 y) were stratified into OW (n = 10; body fat percentage: 34.97 [8.60]) and NW (n = 15; body fat percentage: 18.33 [4.87]). Electromyography was recorded for rectus femoris, vastus lateralis, biceps femoris, gastrocnemius, and tibialis anterior. In addition, the ratings of perceived exertion and range of motion of hip, knee, and ankle joints were collected during stationary exercises. Repeated-measures analysis of variance compared muscle activation, range of motion, and ratings of perceived exertion between groups and exercises. Friedman test examined sequencing of muscles recruitment. Results: Compared with NW, OW experienced significantly greater ratings of perceived exertion (13.7 [0.8] vs 11.7 [0.7]; P < .001) and electromyography amplitude in all muscles apart from vastus lateralis during stationary exercises. In addition, NW children used more consistent muscles’ recruitment pattern in comparison with OW children. The range of motion was similar between groups at all joints. Conclusion: OW children may adopt a more active neuromuscular strategy to provide greater stability and propulsion during stationary exercises. Stationary exercise can be prescribed to strengthen lower extremity muscles in OW children, but mode and intensity must be considered.
Clinical effectiveness of the Manchester Glaucoma Enhanced Referral Scheme
Authors: Gunn Patrick J G , Marks Joanne R , Konstantakopoulou Evgenia , Edgar David F , Lawrenson John G , Roberts Stephen A , Spencer Anne F , Fenerty Cecilia H , Harper Robert A 
Published: October 2018

BackgroundGlaucoma referral filtering schemes have operated in the UK for many years. However, there is a paucity of data on the false-negative (FN) rate. This study evaluated the clinical effectiveness of the Manchester Glaucoma Enhanced Referral Scheme (GERS), estimating both the false-positive (FP) and FN rates.MethodOutcome data were collected for patients newly referred through GERS and assessed in ‘usual-care’ clinics to determine the FP rate (referred patients subsequently discharged at their first visit). For the FN rate, glaucoma suspects deemed not requiring referral following GERS assessment were invited to attend for a ‘reference standard’ examination including all elements of assessment recommended by National Institute for Health and Care Excellence (NICE) by a glaucoma specialist optometrist. A separate 33 cases comprising randomly selected referred and non-referred cases were reviewed independently by two glaucoma specialist consultant ophthalmologists to validate the reference standard assessment.Results1404 patients were evaluated in GERS during the study period; 651 (46.3%) were referred to the Hospital Eye Service (HES) and 753 (53.6%) were discharged. The FP rate in 307 assessable patients referred to the HES was 15.5%. This study reviewed 131 (17.4%) of those patients not referred to the HES through the GERS scheme; 117 (89.3%) were confirmed as not requiring hospital follow-up; 14 (10.7%) required follow-up, including 5 (3.8%) offered treatment. Only one patient (0.8%) in this sample met the GERS referral criteria and was not referred (true FN). There were no cases of missed glaucoma or non-glaucomatous pathology identified within our sample.ConclusionThe Manchester GERS is an effective glaucoma filtering scheme with a low FP and FN rate.
Is establishing a specialist back pain assessment and management service in primary care a safe and effective model? Twelve-month results from the Back pain Assessment Clinic (BAC) prospective cohort pilot study
Authors: Moi John H Y , Phan Uyen , de Gruchy Adam , Liew Danny , Yuen Tanya I , Cunningham John E , Wicks Ian P 
Published: October 2018

ObjectivesTo report on the design, implementation and evaluation of the safety and effectiveness of the Back pain Assessment Clinic (BAC) model.DesignBAC is a new, community-based specialist service for assessing and managing neck and low back pain (LBP). The BAC pilot was supported by a Victorian Department of Health and Human Services grant and was evaluated using the Victorian Innovation Reform Impact Assessment Framework (VIRIAF). Data were obtained by auditing BAC activity (22 July 2014 to 30 June 2015) and conducting surveys and interviews of patients, stakeholders and referrers.SettingTertiary and primary care.ParticipantsAdult patients with neck and LBP referred for outpatient surgical consultation.Main outcome measuresVIRIAF outcomes: (1) access to care; (2) appropriate and safe care; (3) workforce optimisation and integration; and (4) efficiency and sustainability.ResultsA total of 522 patients were seen during the pilot. Most were referred to hospital services by general practitioners (87%) for LBP (63%) and neck pain (24%). All patients were seen within 10 weeks of referral and commenced community-based allied health intervention within 2–4 weeks of assessment in BAC. Of patients seen, 34% had medications adjusted, 57% were referred for physiotherapy, 3.2% to pain services, 1.1% to rheumatology and 1.8% for surgical review. Less MRI scans were ordered in BAC (6.4%) compared with traditional spinal surgical clinics (89.8%), which translated to a cost-saving of $52 560 over 12 months. Patient and staff satisfaction was high. There have been no patient complaints or adverse incidents.ConclusionEvaluation of the BAC pilot suggests it is a potentially safe and cost-saving alternative model of care. Results of the BAC pilot merit further evaluation to determine the potential cost-effectiveness, longer term and broader societal impact of implementing BAC more widely.
Young age at school entry and attention-deficit hyperactivity disorder-related symptoms during primary school: results of a prospective cohort study conducted at German Rudolf Steiner Schools
Authors: Wendt Janine , Schmidt Martina F , König Jochem , Patzlaff Rainer , Huss Michael , Urschitz Michael S 
Published: October 2018

ObjectivesYoung age at school entry (ASE) for students has been related to their impaired mental health in higher grades. To avoid the negative health consequences of young ASE, preschool examinations and individual school entry deferral for young children are routinely performed by some school authorities. We aimed to investigate whether ASE was associated with attention-deficit hyperactivity disorder (ADHD)-related symptoms in pupils attending schools using a selective school enrolment procedure.DesignProspective open cohort study with baseline assessments at school entry and two follow-ups in the second and fourth grades.SettingUp to 128 Rudolf Steiner Schools (Waldorf Schools) located within Germany.ParticipantsOf the 3079 children from whom data were gathered in the second or fourth grade, 2671 children born between 1 July 2001 and 31 October 2002 (age at baseline: mean 6.7, min 5.91, max 7.24 years, 50% girls) were selected for analysis to avoid bias introduced by individuals at the edges of the ASE distribution.Main outcome measuresADHD-related symptoms were assessed at school entry and second and fourth grades by parent-reported and teacher-reported versions of the Strengths and Difficulties Questionnaire (Hyperactivity-Inattention Subscale).ResultsThe agreement between parent-reported and teacher-reported symptoms was poor (intra-class correlation: 0.41 and 0.44 in second and fourth grade assessments, respectively). Regarding teacher reports, ASE was negatively associated with ADHD-related symptoms in the second grade (regression coefficient β=−0.66 per year, P=0.0006) and fourth grade (β=−0.56, P=0.0014). Associations remained after adjusting for potential confounders and pre-existing symptoms at baseline. Regarding parent reports, associations were markedly weaker in both grades (second grade: β=−0.22, P=0.12; fourth grade: β=−0.09, P=0.48).ConclusionsUsing a prospective study design and comprehensive adjustment for confounding and baseline symptoms, we confirmed prior evidence of the association between young ASE and teacher-reported ADHD symptoms in primary school.
Work-life balance behaviours cluster in work settings and relate to burnout and safety culture: a cross-sectional survey analysis
Authors: Schwartz Stephanie P , Adair Kathryn C , Bae Jonathan , Rehder Kyle J , Shanafelt Tait D , Profit Jochen , Sexton J Bryan 
Published: October 2018

BackgroundHealthcare is approaching a tipping point as burnout and dissatisfaction with work-life integration (WLI) in healthcare workers continue to increase. A scale evaluating common behaviours as actionable examples of WLI was introduced to measure work-life balance.Objectives(1) Explore differences in WLI behaviours by role, specialty and other respondent demographics in a large healthcare system. (2) Evaluate the psychometric properties of the work-life climate scale, and the extent to which it acts like a climate, or group-level norm when used at the work setting level. (3) Explore associations between work-life climate and other healthcare climates including teamwork, safety and burnout.MethodsCross-sectional survey study completed in 2016 of US healthcare workers within a large academic healthcare system.Results10 627 of 13 040 eligible healthcare workers across 440 work settings within seven entities of a large healthcare system (81% response rate) completed the routine safety culture survey. The overall work-life climate scale internal consistency was α=0.830. WLI varied significantly among healthcare worker role, length of time in specialty and work setting. Random effects analyses of variance for the work-life climate scale revealed significant between-work setting and within-work setting variance and intraclass correlations reflected clustering at the work setting level. T-tests of top versus bottom WLI quartile work settings revealed that positive work-life climate was associated with better teamwork and safety climates, as well as lower personal burnout and burnout climate (p<0.001).ConclusionProblems with WLI are common in healthcare workers and differ significantly based on position and time in specialty. Although typically thought of as an individual difference variable, WLI appears to operate as a climate, and is consistently associated with better safety culture norms.
Neurofilament light chain in serum for the diagnosis of amyotrophic lateral sclerosis
Authors: Verde Federico , Steinacker Petra , Weishaupt Jochen H , Kassubek Jan , Oeckl Patrick , Halbgebauer Steffen , Tumani Hayrettin , von Arnim Christine A F , Dorst Johannes , Feneberg Emily , Mayer Benjamin , Müller Hans-Peter , Gorges Martin , Rosenbohm Angela , Volk Alexander E , Silani Vincenzo , Ludolph Albert C , Otto Markus 
Published: October 2018

ObjectiveTo determine the diagnostic and prognostic performance of serum neurofilament light chain (NFL) in amyotrophic lateral sclerosis (ALS).MethodsThis single-centre, prospective, longitudinal study included the following patients: 124 patients with ALS; 50 patients without neurodegenerative diseases; 44 patients with conditions included in the differential diagnosis of ALS (disease controls); 65 patients with other neurodegenerative diseases (20 with frontotemporal dementia, 20 with Alzheimer’s disease, 19 with Parkinson’s disease, 6 with Creutzfeldt-Jakob disease (CJD)). Serum NFL levels were measured using the ultrasensitive single molecule array (Simoa) technology.ResultsSerum NFL levels were higher in ALS in comparison to all other categories except for CJD. A cut-off level of 62 pg/mL discriminated between ALS and all other conditions with 85.5% sensitivity (95% CI 78% to 91.2%) and 81.8% specificity (95% CI 74.9% to 87.4%). Among patients with ALS, serum NFL correlated positively with disease progression rate (rs=0.336, 95% CI 0.14 to 0.506, p=0.0008), and higher levels were associated with shorter survival (p=0.0054). Serum NFL did not differ among patients in different ALS pathological stages as evaluated by diffusion-tensor imaging, and in single patients NFL levels were stable over time.ConclusionsSerum NFL is increased in ALS in comparison to other conditions and can serve as diagnostic and prognostic biomarker. We established a cut-off level for the diagnosis of ALS.
Interpretation of regional-scale distribution of high Hg in soils of karst area in southwest China
Authors: Nie Lanshi , Liu Xuemin , Wang Xueqiu , Liu Hanliang , Wang Wei 
Published: October 2018

The abundance and spatial distribution of mercury in soil samples covering nearly the whole of China (
c
. 9 million km
2
) were obtained by the China Geochemical Baseline (CGB) project from 2008 to 2015. From the data and maps, it was found that the Hg contents both in the top (0–25 cm) and deep soil (below 100 cm or C-horizon) of the karst area in southwestern China are richer than that of any other geographical landscape areas. Median Hg values for this karst area are 113 and 90 µg/kg respectively (
n
= 126), in the top and bottom soils, much higher than the corresponding data of 26 and 18 µg/kg for the entire country (
n
= 3380). This regional-scale high Hg distribution is due mainly to high Hg geochemical backgrounds in bedrock and soil formation on limestone. A large-scale epithermal metallogenic domain developed in southwestern China and the extensive distribution of Lower Cambrian black shale in southwest China led to a high Hg geochemical background for parent rocks of soils. The chemical weathering of carbonate minerals and the geochemical behaviour of Hg in the epigenetic environment result in Hg enrichment by ferric oxides, iron hydroxide and organic carbon in limestone soils. Human activities mostly result in local-scale Hg anomalies, but have little effect on this regional-scale anomaly.
Experimental myofascial trigger point creation in rodents
Authors: Margalef Ramon , Sisquella Marc , Bosque Marc , Romeu Clara , Mayoral Orlando , Monterde Sonia , Priego Mercedes , Guerra-Perez Rafael , Ortiz Nicolau , Tomàs Josep , Santafe Manel M. 
Published: October 2018

Myofascial pain syndrome is one of the most common forms of muscle pain. In this syndrome, pain is originated by the so-called trigger points, which consists of a set of palpable contraction knots in the muscle. It has been proposed that a high, spontaneous neurotransmission may be involved in the generation of these contraction knots. To confirm this hypothesis, we exposed mouse muscles to an anticholinesterasic agent to increase the neurotransmision in the synaptic cleft in two different conditions, in vivo and ex vivo experiments. Using intracellular recordings, a sharp increase in the spontaneous neurotransmission in the levator auris longus muscle and a lower increase in the diaphragm muscle could be seen. Likewise, electromyography recordings reveal an elevated endplate noise in gastrocnemius muscle of treated animals. These changes are associated with structural changes such as abundant neuromuscular contracted zones observed by rhodaminated α-bungarotoxin and the presence of abundant glycosaminoglycans around the contraction knots, as shown by Alcian PAS staining. In a second set of experiments, we aimed at demonstrating that the increases in the neurotransmission reproduced most of the clinical signs associated to a trigger point. We exposed rats to the anticholinesterase agent neostigmine, and 30 min afterward we observed the presence of palpable taut bands, the echocardiographic presence of contraction knots, and local twitch responses upon needle stimulation. In summary, we demonstrated that increased neurotransmission induced trigger points in both rats and mice, as evidenced by glycosaminoglycans around the contraction zones as a novel hallmark of this pathology.NEW & NOTEWORTHY In rodents, when neostigmine was injected subcutaneously, the neuromuscular neurotransmission increased, and several changes can be observed: an elevated endplate noise compared with normal endplate noise, as evidenced by electromyographyc recording; many muscular fibers with contraction knots (narrower sarcomeres and locally thickened muscle fiber) surrounded by infiltration of connective tissue like glycosaminoglycans molecules; and palpable taut bands and local twitch responses upon needle stimulation. Several of these signs are also observed in humans with muscle pain.