bluuebunny/crossref_metadata_2025_split huggingface dataset:
The age-regulated zinc finger factor ZNF367 is a new modulator of neuroblast proliferation during embryonic neurogenesis
Authors: Naef Valentina , Monticelli Sara , Corsinovi Debora , Mazzetto Maria Teresa , Cellerino Alessandro , Ori Michela 
Published: August 2018

AbstractGlobal population aging is one of the major social and economic challenges of contemporary society. During aging the progressive decline in physiological functions has serious consequences for all organs including brain. The age-related incidence of neurodegenerative diseases coincides with the sharp decline of the amount and functionality of adult neural stem cells. Recently, we identified a short list of brain age-regulated genes by means of next-generation sequencing. Among them znf367 codes for a transcription factor that represents a central node in gene co-regulation networks during aging, but whose function in the central nervous system (CNS), is completely unknown. As proof of concept, we analysed the role of znf367 during Xenopus laevis neurogenesis. By means of a gene loss of function approach limited to the CNS, we suggested that znf367 might act as a key controller of the neuroblast cell cycle, particularly in the progression of mitosis and spindle checkpoint. A candidate gene approach based on a weighted-gene co-expression network analysis, revealed fancd2 and ska3 as possible targets of znf367. The age-related decline of znf367 correlated well with its role during embryonic neurogenesis, opening new lines of investigation also in adult neurogenesis to improved maintenance and even repair of neuronal function.
Positive/Negative Allosteric Modulation Switching in an Umami Taste Receptor (T1R1/T1R3) by a Natural Flavor Compound, Methional
Authors: Toda Yasuka , Nakagita Tomoya , Hirokawa Takatsugu , Yamashita Yuki , Nakajima Ayako , Narukawa Masataka , Ishimaru Yoshiro , Uchida Riichiro , Misaka Takumi 
Published: August 2018

AbstractTaste is a vital sensation for vertebrates, enabling the detection of nutritionally important substances or potential toxins. A heteromeric complex of two class C GPCRs, T1R1 and T1R3, was identified as the umami (savory) taste receptor. Amino acids and 5′-ribonucleotides are well known to be natural ligands for human T1R1/T1R3. In this study, we reveal that methional, which is a familiar flavor component in foods, is an allosteric modulator of T1R1/T1R3. Receptor expression experiments showed that methional served as a positive allosteric modulator (PAM) of human T1R1/T1R3 and functioned as a negative allosteric modulator (NAM) of mouse T1R1/T1R3. Although amino acids and 5′-ribonucleotides bound to the extracellular domain of T1R1, the use of interspecies chimeric receptors demonstrated that methional interacted with the transmembrane domain of T1R1. Site-directed mutagenesis and molecular modeling showed that methional could potentially bind at two distinct sites in the transmembrane domain of T1R1 and that the amino acid residues in the bottom of the allosteric pocket engendered the switch between the PAM and NAM modes, which could contribute to switching the binding position of methional. These results may be applicable for elucidating the molecular mechanisms underlying ligand recognition by other class C GPCRs.
Development of heme protein based oxygen sensing indicators
Authors: Nomata Jiro , Hisabori Toru 
Published: August 2018

AbstractOxygen is essential for aerobic life and is required for various oxygen-dependent biochemical reactions. In addition, oxygen plays important roles in multiple intracellular signaling pathways. Thus, to investigate oxygen homeostasis in living cells, we developed a genetically encoded oxygen sensor protein using the oxygen sensor domain of bacterial phosphodiesterase direct oxygen sensor protein (DosP), which was connected to yellow fluorescence protein (YFP) using an optimized antiparallel coiled-coil linker. The resulting ANA-Y (Anaerobic/aerobic sensing yellow fluorescence protein) was highly sensitive to oxygen and had a half saturation concentration of 18 μM. The ANA-Y reacts with dissolved oxygen within 10 s and the resulting increases in fluorescence are reversed with decreases in oxygen concentrations. This sensitivity of the ANA-Y enabled direct determinations of initial photosynthetic oxygen production by cyanobacteria. ANA-Y exhibits reversible fluorescence change of donor YFP following reversible absorbance change of acceptor DosH, and the operating mechanism of this ANA-Y could be used to develop various protein sensor probes for intracellular signaling molecules using natural sensor proteins.
CT textural analysis of gastric cancer: correlations with immunohistochemical biomarkers
Authors: Liu Shunli , Shi Hua , Ji Changfeng , Guan Wenxian , Chen Ling , Sun Yingshi , Tang Lei , Guan Yue , Li Weifeng , Ge Yun , He Jian , Liu Song , Zhou Zhengyang 
Published: August 2018

AbstractTo investigate the ability of CT texture analysis to assess and predict the expression statuses of E-cadherin, Ki67, VEGFR2 and EGFR in gastric cancers, the enhanced CT images of 139 patients with gastric cancer were retrospectively reviewed. The region of interest was manually drawn along the margin of the lesion on the largest slice in the arterial and venous phases, which yielded a series of texture parameters. Our results showed that the standard deviation, width, entropy, entropy (H), correlation and contrast from the arterial and venous phases were significantly correlated with the E-cadherin expression level in gastric cancers (all P < 0.05). The skewness from the arterial phase and the mean and autocorrelation from the venous phase were negatively correlated with the Ki67 expression level in gastric cancers (all P < 0.05). The width, entropy and contrast from the venous phase were positively correlated with the VEGFR2 expression level in gastric cancers (all P < 0.05). No significant correlation was found between the texture features and EGFR expression level. CT texture analysis, which had areas under the receiver operating characteristic curve (AUCs) ranging from 0.612 to 0.715, holds promise in predicting E-cadherin, Ki67 and VEGFR2 expression levels in gastric cancers.
Exercise prevents impaired autophagy and proteostasis in a model of neurogenic myopathy
Authors: Campos Juliane C. , Baehr Leslie M. , Gomes Kátia M. S. , Bechara Luiz R. G. , Voltarelli Vanessa A. , Bozi Luiz H. M. , Ribeiro Márcio A. C. , Ferreira Nikolas D. , Moreira José B. N. , Brum Patricia C. , Bodine Sue C. , Ferreira Julio C. B. 
Published: August 2018

AbstractIncreased proteolytic activity has been widely associated with skeletal muscle atrophy. However, elevated proteolysis is also critical for the maintenance of cellular homeostasis by disposing cytotoxic proteins and non-functioning organelles. We recently demonstrated that exercise activates autophagy and re-establishes proteostasis in cardiac diseases. Here, we characterized the impact of exercise on skeletal muscle autophagy and proteostasis in a model of neurogenic myopathy induced by sciatic nerve constriction in rats. Neurogenic myopathy, characterized by progressive atrophy and impaired contractility, was paralleled by accumulation of autophagy-related markers and loss of acute responsiveness to both colchicine and chloroquine. These changes were correlated with elevated levels of damaged proteins, chaperones and pro-apoptotic markers compared to control animals. Sustained autophagy inhibition using chloroquine in rats (50 mg.kg−1.day−1) or muscle-specific deletion of Atg7 in mice was sufficient to impair muscle contractility in control but not in neurogenic myopathy, suggesting that dysfunctional autophagy is critical in skeletal muscle pathophysiology. Finally, 4 weeks of aerobic exercise training (moderate treadmill running, 5x/week, 1 h/day) prior to neurogenic myopathy improved skeletal muscle autophagic flux and proteostasis. These changes were followed by spared muscle mass and better contractility properties. Taken together, our findings suggest the potential value of exercise in maintaining skeletal muscle proteostasis and slowing down the progression of neurogenic myopathy.
Orthographic Fast-Mapping Across Time in 5- and 6-Year-Old Children
Authors: Henbest Victoria S. , Apel Kenn 
Published: August 2018

Purpose
The purpose of this study was to examine the orthographic fast-mapping abilities of 5- and 6-year-old children across time to determine (a) growth in the ability to quickly acquire mental images of written words, (b) the effect of words' statistical regularities on the learning of written word images across time, (c) whether the statistical regularities of words impact children's eye movements during an orthographic fast-mapping task, and (d) the relation among written word learning and future literacy skills.

Method
Twenty-eight 5- and 6-year-old children viewed and listened to 12 short stories while their eye movements were recorded across 2 time points (approximately 3 months apart). At each time point, objects in the stories represented 12 novel pseudowords differing in their phonotactic and orthotactic probabilities. After viewing each story, the children were asked to spell and identify the target pseudowords; they also completed a battery of literacy measures.

Results
The children were able to quickly acquire mental orthographic representations of the novel written pseudowords as evidenced by their ability to identify and spell the target pseudowords after viewing the stories. This ability was related to future literacy performance and significantly improved over time. Performance on the orthographic fast-mapping tasks and the children's eye movements at Time 2 were influenced by the words' linguistic properties.

Conclusions
This study adds to accumulating evidence that orthographic fast-mapping is largely influenced by the orthotactic probabilities of words. These findings, taken together with those from previous investigations, provide a rich amount of evidence indicating that children are statistical learners when developing their orthographic knowledge.
Pseudo-photometric distances of 30 open clusters
Authors: Chelli A. , Duvert G. 
Published: August 2018

Aims. We demonstrate that reliable photometric distances of stellar clusters, and more generally of stars, can be obtained using pseudomagnitudes and rough spectral type without having to correct for visual absorption.
Methods. We determine the mean absolute pseudomagnitude of all spectral (sub)types between B and K. Distances are computed from the difference between the star’s observed pseudomagnitude and its spectral type’s absolute pseudomagnitude. We compare the distances of 30 open clusters thus derived against the distances derived from TGAS parallaxes.
Results. Our computed distances, up to distance modulus 12, agree within 0.1 mag rms with those obtained from TGAS parallaxes, proving excellent distance estimates. We show additionally that there are actually two markedly different distances in the cluster NGC 2264.
Conclusions. We suggest that the pseudomagnitude distance estimation method, which is easy to perform, can be routinely used in all large-scale surveys where statistical distances on a set of stars, such as an open cluster, are required.
Simulating the pericentre passage of the Galactic centre star S2
Authors: Schartmann M. , Burkert A. , Ballone A. 
Published: August 2018

Context. Our knowledge of the density distribution of the accretion flow around Sgr A* – the massive black hole (BH) at our Galactic centre (GC) – relies on two measurements only: one at a distance of a few Schwarzschild radii (Rs) and one at roughly 105 Rs, which are usually bridged by a power law, which is backed by magnetohydrodynamical simulations. The so-called S2 star reached its closest approach to the massive BH at around 1500 Rs in May 2018. It has been proposed that the interaction of its stellar wind with the high-density accretion flow at this distance from Sgr A* will lead to a detectable, month-long X-ray flare.
Aims. Our goal is to verify whether or not the S2 star wind can be used as a diagnostic tool to infer the properties of the accretion flow towards Sgr A* at its pericentre (an unprobed distance regime), putting important constraints on BH accretion flow models. Methods. We run a series of three-dimensional adaptive mesh refinement simulations with the help of the RAMSES code which include the realistic treatment of the interaction of S2’s stellar wind with the accretion flow along its orbit and – apart from hydrodynamical and thermodynamical effects – include the tidal interaction with the massive BH. These are post-processed to derive the X-ray emission in the observable 2–10 keV window.
Results. No significant excess of X-ray emission from Sgr A* is found for typical accretion flow models. A measurable excess is produced for a significantly increased density of the accretion flow. This can, however, be ruled out for standard power-law accretion flow models as in this case the thermal X-ray emission without the S2 wind interaction would already exceed the observed quiescent luminosity. Only a significant change of the wind parameters (increased mass loss rate and decreased wind velocity) might lead to an (marginally) observable X-ray flaring event.
Conclusion. Even the detection of an (month-long) X-ray flare during the pericentre passage of the S2 star would not allow for strict constraints to be put on the accretion flow around Sgr A* due to the degeneracy caused by the dependence on multiple parameters (of the accretion flow model as well as the stellar wind).
A reevaluation of the 2MASS zero points using CALSPEC spectrophotometry complemented with Gaia Data Release 2 parallaxes
Authors: Maíz Apellániz J. , Pantaleoni González M. 
Published: August 2018

Context. 2MASS is the reference survey in the near-infrared (NIR) part of the spectrum due to its whole-sky coverage, large dynamic range, and proven calibration uniformity. However, previous studies disagree on the value of the zero points (ZPs) for its three bands JHK at the hundredth of a magnitude level. The disagreement should become more noticeable now that Gaia provides whole-sky optical photometry calibrated below that level.
Aims. We want to establish the value of the 2MASS ZPs based on NICMOS/HST spectrophotometry of the CALSPEC standard stars and test it with the help of Gaia DR2 parallaxes.
Methods. We have computed the synthetic JHK photometry for a sample of stars using the HST CALSPEC spectroscopic standards and compared it with their 2MASS magnitudes to evaluate the ZPs. We have tested our results by analyzing a sample of FGK dwarfs with excellent 2MASS photometry and accurate Gaia DR2 parallaxes.
Results. The Vega ZPs for 2MASS J, H, and K are found to be −0.025 ± 0.005, 0.004 ± 0.005, and −0.015 ± 0.005 mag, respectively. The analysis of the FGK sample indicates that the new ZPs are more accurate than previous ones.
Qui initiera la transformation digitale de la santé?
Authors: Gryson Olivier 
Published: August 2018

Depuis quelques années fleurissent une multitude d’initiatives digitales médicales qui affichent de tangibles signaux d’efficacité. Elles placent souvent le patient au cœur de leur approche et s’affranchissent, tout au moins conceptuellement, des silos entre les grands acteurs de la santé (industrie pharmaceutique, autorités de santé, hôpital, payeurs).Il en découle une fragilité structurelle susceptible de constituer un terreau favorable à l’arrivée d’acteurs issus du monde numérique, des « pure players », capables de repenser et de transformer radicalement les modèles économiques en proposant des alternatives digitales de rupture centrées sur les besoins des patients.
Accuracy of Suction Rectal Biopsy for Diagnosis of Hirschsprung's Disease in Neonates
Authors: Allen Ashley Rebekah , Putnam Angelica R. , Presson Angela P. , Allen Chelsea McCarty , Barnhart Douglas C. , Rollins Michael D. 
Published: August 2018

Introduction Recent publications have questioned the sensitivity of suction rectal biopsy (SRB) for diagnosis of Hirschsprung's disease (HD) in newborns. A recent European survey reported that 39% of pediatric surgeons performed full-thickness transanal biopsies due to concerns about the accuracy of SRB. We sought to examine our contemporary SRB experience in infants.
Materials and Methods A review was performed (2007–2016) of patients under 6 months of age who had a SRB at our children's hospital. The cohort was subdivided by postmenstrual age at time of SRB: preterm (< 40 weeks, A), term neonate (40–44 weeks, B), and infant (> 44 weeks, C). The pathology reports from endorectal pull-through were used as gold standard confirmation. One-year follow-up of patients with negative SRB was used to confirm accurate diagnosis.
Results A total of 153 patients met the criteria and a total of 159 SRBs (< 2,500 g; n = 26) were performed (A = 60, B = 58, C = 35). Forty-three patients were diagnosed with HD (A = 25, B = 15, C = 3). A second SRB was performed in 6 (3.9%) patients due to inadequate tissue (A = 2, B = 2, C = 2) with HD diagnosed in 5. No complications occurred. Sensitivity and specificity of SRB was 100% in all age groups. Half of the patients with a negative SRB had at least 1 year follow-up, with none subsequently diagnosed with HD.
Conclusion SRB results in adequate tissue for evaluation of HD in nearly all patients less than 6 months of age on the first attempt and is highly accurate in the preterm and newborn infants. No complications occurred, even among infants less than 2,500 g.
A Rare Case of Septic Arthritis of Lumbar Facet Joint with Epidural Abscess, and Bacterial Meningitis Caused by Methicillin-Sensitive Staphylococcus aureus
Authors: Maldonado-Morán Miguel , Muñoz Juan , Matta Javier , Arrieta Victor , Bernal Oscar , Mejía Jairo 
Published: August 2018

AbstractSeptic arthritis of the facet joint is an extremely rare condition, even more in young immunocompetent patients. There have been approximately only 40 cases of this entity reported worldwide. Here, the authors present a 16-year-old male patient with lower back pain, fever, and cephalea. Blood cultures and cerebrospinal fluid analysis confirmed methicillin-sensitive Staphylococcus aureus infection, and magnetic resonance imaging showed septic arthritis of the lumbar left facet joint L3 and epidural abscess. Intravenous oxacillin was administered, and the patient improved. No other treatment was required.
Input-output effects in the bilingual first language acquisition of English and Polish
Authors: Gaskins Dorota 
Published: August 2018

Abstract
This diary study looks at the acquisition of early words in two bilingual sisters (0;9–2;03.22 and 0;9–1;09.13) exposed to English and Polish from birth. It examines whether their parents’ input recorded on video can explain the proportions of different types of words learnt. Their bias for social words is explained by these words being heard in isolation; that for nouns by competitive proportions of noun types heard in the input. Contrarily, the late acquisition of closed-class items is explained by their high usage rates as part of constructions. Meanwhile, high numbers of early verbs in both children’s Polish are explained by inflected Polish verbs being heard (a) in isolation and (b) at the beginning and end of utterances more frequently than their uninflected English counterparts. These results are discussed within the context of the usage-based theory, with focus on the impact of word types for the acquisition of word groups.
Simultaneous detection of eight species of tree nut in foods using two tetraplex polymerase chain reaction assays
Authors: Ito Mina , Mizota Taisei , Kitaguchi Takashi , Ohno Katsutoshi , Ohba Tetsuro , Tanaka Mitsuru 
Published: August 2018

ABSTRACT
Tree nuts comprise a category of food allergens that must be included in the food labels in several countries. We developed a polymerase chain reaction (PCR) method using eight specific primer pairs to detect eight representative tree nuts (almond, Brazil nut, cashew, hazelnut, macadamia nut, pecan, pistachio, and walnut) under the same experimental conditions. The specificity of the eight primer pairs was confirmed by PCR testing against a variety of plant and animal samples. The detection limit of the method ranged from 1 fg to 1 pg DNA of individual tree nuts. The method detected tree nut DNA in processed and unprocessed food. In addition, the primer pairs could be combined into two sets of tetraplex PCR system. The developed method is specific, sensitive, and efficient, making it useful for detecting trace amounts of eight species of tree nut in foods.
Gender Differences in Spousal Caregivers’ Care and Housework: Fact or Fiction?
Authors: Langner Laura A , Furstenberg Frank F 
Published: August 2018

AbstractObjectiveMany studies reveal a gender gap in spousal care during late life. However, this gap could be an artifact of methodological limitations (small and unrepresentative cross-sectional samples). Using a data set that overcomes these limitations, we re-examine the question of gender differences in spousal care and housework adjustment when a serious illness occurs.MethodWe use biannual waves between 2001 and 2015 of the German Socio-Economic Panel Study and growth curve analyses. We follow couples longitudinally (identified in the household questionnaire) to analyze shifts in spousal care hours and housework plus errand hours that occur as a response to the spousal care need. We test for interactions with levels of care need and with gender.ResultsWe found that men increase their care hours as much as women do, resulting in similar care hours. They also increase their housework and errand hours more than women do. Yet at lower levels of spousal care need, women still do more housework and errands because they spent more time doing housework before the illness.DiscussionEven in a context of children’s decreasing availability to care for parents, male spouses assume the required caregiving role in systems relying on a mixture of public and private care.
The short orbital period binary star at the heart of the planetary nebula M 3-1
Authors: Jones David , Boffin Henri M J , Sowicka Paulina , Miszalski Brent , Rodríguez-Gil Pablo , Santander-García Miguel , Corradi Romano L M 
Published: August 2018

ABSTRACT
We present the discovery of a 3h5m orbital-period binary star at the heart of the planetary nebula M 3-1 – the shortest period photometrically variable central star known and second only to V458 Vul, in general. Combined modelling of light and radial velocity curves reveals both components to be close to Roche lobe filling, strongly indicating that the central star will rapidly evolve to become a cataclysmic variable, perhaps experiencing a similar evolution to V458 Vul resulting in a nova eruption before the planetary nebula has fully dissipated. While the short orbital period and near Roche lobe filling natures of both components make the central binary of M 3-1 an important test case with which to constrain the formation processes of cataclysmic variables, novae, and perhaps even supernovae type Ia.