bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Long Non‐Coding RNA as Potential Biomarkers for the Diagnosis of Postmenopausal Osteoporosis
Authors: Yihua Zhu , Chenjian Peng , Lining Wang , Shujun Jiang , Haomiao Yang , Kaixuan Zhang , Fenglei Dai , Yong Ma , Xudong Chu , Chunlei Zhang , Haitao Sun 
Published: November 2024

Objective: To explore the feasibility and clinical application value of differentially expressed lncRNA in human peripheral blood mononuclear cell (PBMC) as a potential biomarker for postmenopausal osteoporosis (PMOP).Methods: In this study, a case‐control trial was conducted to collect a total of 10 samples of PBMC from PMOP and postmenopausal‐without‐osteoporosis (n‐PMOP) patients. RNA sequencing was performed to profile lncRNA and mRNA expression, identifying differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs) based on the criteria of fold change (FC) ≥ 2 and p value < 0.05; GO and KEGG enrichment analyses were carried out for differentially expressed genes (DEGs); 10 DElncRNAs and 20 DEmRNAs were selected for lncRNA–mRNA correlation analysis and Circos plot to screen out the lncRNAs that could be used as potential biomarkers. Then, ROC curve analysis was used to evaluate the diagnostic and therapeutic value of DElncRNAs as clinical potential diagnostic markers for PMOP. Afterward, 20 PMOP and 20 n‐PMOP patients were reincluded and quantitative real‐time PCR (qRT‐PCR) was performed to externally validate the screening of lncRNAs.Result: (1) Compared with n‐PMOP, there were 1978 DElncRNAs and 1024 DEmRNAs in PMOP patients. (2) Bioinformatics technology was used to analyze the DEGs, and the GO analysis showed that the activities of the gene products were mainly related to the protein binding, membrane, plasma membrane, and extracellular region. The results of KEGG enrichment analysis showed that it was mainly enriched in PI3K‐Akt signaling pathway, metabolic pathways, and pathways in cancer and focal adhesion. (3) The correlation network and Circos plot further indicated the implication of DElncRNA expression profiles in PMOP via interactions with DEmRNAs. Among them, lncRNA RAB37, lncRNA BEGAIN, and lncRNA ZNF529 had the highest number of nodes, totaling 19, possibly potential diagnostic markers for PMOP. (4) The diagnostic efficacy of the screened lncRNAs was analyzed by ROC curve. The results showed an the area under the ROC curve (AUC) of 0.960 for lncRNA RAB37, 1.000 for lncRNA ZNF529, 1.000 for lncRNA BEGAIN. (5) The qPCR results showed that lncRNA RAB37, lncRNA ZNF529, and lncRNA BEGAIN were all significantly correlated with the occurrence of PMOP (p < 0.05). However, the significant difference of lncRNA ZNF529 was superior to that of other lncRNAs.Conclusion: The lncRNA ZNF529 is significantly overexpressed in PBMC in PMOP, and bioinformatics analysis and validation experiments indicate that it is closely associated with PMOP; thus, it is expected to be a potential diagnostic marker for PMOP.
Diabetic Cardiomyopathy Uncovered: Transcriptomics, NLRP3, and Carvedilol Mechanisms
Authors: Abudoureyimu Alimujiang , Aihaiti Alimu , Abudoujilili Nuliman , Tuergong Mayila , Adili Guzainuer , Maimaiti Maihebubaimu , Mohetaer Dilinuer , Maiamaitishawuti Yimamumaimaiti 
Published: November 2024

Background: This study investigates the impact of a high‐sugar environment on H9C2 cardiomyocytes and explores the protective effects of carvedilol in the context of diabetic cardiomyopathy (Dia‐CM). Transcriptomic analysis identified 21,655 differentially expressed genes associated with Dia‐CM, demonstrating significant separation among samples.Methods: H9C2 cardiomyocytes were cultured in a high‐sugar environment to simulate Dia‐CM conditions. Cell viability, cytokine levels, and protein expression were assessed using CCK‐8 assays, ELISA, and Western blot techniques. Intervention experiments with NLRP3, caspase‐1, and ROS inhibitors were conducted to evaluate their protective effects. The therapeutic potential of carvedilol was assessed by examining its impact on cell viability, cytokine levels, and key biomarkers. An in‐depth analysis of carvedilol’s regulatory effects on ROS and key proteins in H9C2 cells was also conducted.Results: In vitro, a high‐sugar environment significantly reduced H9C2 cell survival, increased ROS levels, activated inflammatory responses, and upregulated NLRP3, caspase‐1, and GSDMD‐N proteins. Inhibitors of NLRP3, caspase‐1, and ROS ameliorated these effects. Carvedilol treatment improved cell activity, reduced inflammatory cytokine levels, suppressed ROS production, and downregulated NLRP3, pro‐caspase‐1, GSDMD‐N, and p‐NF‐κB proteins. Moderate‐dose carvedilol exhibited optimal intervention effects.Conclusions: A high‐sugar environment induces cardiomyocyte damage through ROS production and NLRP3 inflammasome activation. Inhibitors of NLRP3, caspase‐1, and ROS provide effective protection. Carvedilol significantly mitigates the detrimental effects of a high‐sugar environment on H9C2 cardiomyocytes, potentially through inhibiting the NLRP3‐ASC inflammasome and caspase‐1/GSDMD‐dependent signaling pathway‐mediated pyroptosis. These findings offer insights into Dia‐CM mechanisms and highlight carvedilol as a promising therapeutic intervention.
Patient‐Reported Experience Measures in Adult Inpatient Settings: A Systematic Review
Authors: Kang Yichen , Guan Tingyu , Chen Xiao , Zhang Yuxia 
Published: November 2024

Background: Patient‐centered ideas have become the key indicator of medical service quality, and patient‐reported experience measures are ways to measure how well this idea is being implemented. There are currently numerous adult inpatient experience instruments available, and it is necessary to conduct such systematic reviews to discover any new instruments and help policymakers and researchers increase the likelihood of hearing true patients’ voices through appropriate selection of these instruments.Objective: To identify existing adult inpatient experience measures and to critically appraise their development design and psychometric testing results.Methods: EMBASE, PUBMED, Cochrane, CINAHL (EBSCOhost), PsycINFO, and ProQuest were searched from inception to March 2023. A comprehensive review following the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines was conducted. Studies were identified via specific search terms and inclusion criteria. The methodological quality assessment was evaluated according to the COnsensus‐based Standards for the selection of health Measurement INstruments (COSMIN) checklist.Results: A total of 29 articles reporting on 23 instruments were included. Each instrument demonstrated both satisfaction and disappointment during the development process and psychometric testing with the recommended criteria of the COSMIN checklist. Pilot tests and cognitive interviews were ignored or not reported in 9 studies. Only 5 studies evaluated the content validity. Among all measurement properties, internal consistency and structural validity were the two most frequently measured attributes. None of the 29 included studies assessed the responsiveness or measurement error of the scales.Conclusion: Among a variety of adult inpatient experience instruments, only a limited number of studies were methodologically sound. Further research still needs to be conducted for the development and validation of patient‐reported experience measures. New quality assessments, such as instrument utility, also should be implemented to provide a more complete evaluation of instruments in the information era.
Promotor Hypomethylation Mediated Upregulation of VCAN Targets Twist1 to Promote EndMT in Hypoxia‐Induced Pulmonary Hypertension
Authors: Yu Jinyan , Hong Shanchao , Yang Lingjia , Ye Shugao , Yu Zhen , Zhang Zheming , Wang Ziteng , Huang Shulun , Chen Yuan , Bian Tao , Wu Yan 
Published: November 2024

Background
Hypoxia‐induced pulmonary hypertension (HPH) is a severe vascular disorder that is characterized by the involvement of endothelial‐to‐mesenchymal transition (EndMT) in its pathogenesis. Our previous research has suggested that the gene versican may have a crucial role in the development of HPH. However, the exact function of versican in HPH requires further investigation.

Methods and Results
The expression of versican and markers of EndMT was assessed using Western blot, immunohistochemistry, and immunofluorescence. Vascular remodeling and right ventricular hypertrophy in patients with HPH and mice were evaluated through hematoxylin and eosin staining, Masson's staining, and hemodynamic measurements. Protein interactions were validated using co‐immunoprecipitation, and the DNA methylation level of versican was examined using methylation‐specific polymerase chain reaction. Compared with the control, EndMT was observed in patients with HPH, HPH mouse models, and hypoxia‐treated human pulmonary artery endothelial cells, accompanied by a significant increase of versican. Endothelium‐specific knockdown of versican reversed HPH progression and effectively prevented EndMT in mouse models and human pulmonary artery endothelial cells. We further confirmed that versican participated in EndMT by targeting the key transcription factor Twist1. Additionally, the upregulation of versican may be attributed to promoter hypomethylation, which was mediated by reduced DNA methyltransferases activity under hypoxic conditions.

Conclusions
This study provides the initial evidence showcasing the role of promoter hypomethylation‐mediated versican upregulation in promoting EndMT by targeting Twist1, which facilitates vascular remodeling and the progression of HPH. These findings offer a promising new target for the treatment of HPH.
A Short Appendix to Richard Lavenham’s Tractatus terminorum naturalium
Authors: Hanke Miroslav 
Published: November 2024

Abstract
Continuing the tradition of Oxford Calculatorial physics, the late fourteenth-century British Carmelite Richard Lavenham authored an amplification of the popular short textbook commonly referred to as Termini naturales. A copy of this amplification, preserved in a late fourteenth-century manuscript (Venezia, Biblioteca Nazionale Marciana, ms Lat. Z. 300 [= 1872]), is followed by a short series of notes and comments on some of the principles introduced in Lavenham’s treatise (drawn from the first four books of Aristotle’s Physics). The present article offers an edition of the text, and analyses its content and relationship with fourteenth-century physics, especially with the physical doctrines of Richard Lavenham.
Efficacy of banana fibre paper for the management of the root-knot nematode, Meloidogyne incognita, on potato (Solanum tuberosum) in Kenya
Authors: Kamau Dorris W. , Waceke Wanjohi , Cortada Laura , Mwaura Onesmus , Pirzada Tahira , Sit Tim L. , Davis Eric , Guenther Dick , Mathew Reny , Pal Lokendra , Khan Saad , Haukeland Solveig , Kisaakye James , Opperman Charles , Coyne Danny 
Published: November 2024

Summary
Globally, potato (Solanum tuberosum) is a key staple food crop. In Kenya, it is the second most important food crop after maize. Among the various constraints to potato production are plant-parasitic nematodes. In particular, root-knot nematodes (Meloidogyne spp.) are a significant impediment to potato production, suppressing yield and reducing the quality of harvested tubers. The current study was undertaken to evaluate the efficacy of a lignocellulose fibre matrix (banana paper) either impregnated with a chemical or drenched with a biologically-based nematicide for the management of root-knot nematodes on potatoes, in Kenya. The experiment was conducted in both field and pot trials over two consecutive cropping seasons. Wrapping seed potatoes in banana paper impregnated with abamectin or drenched with Trichoderma asperellum (Real Trichoderma®) led to suppression of soil Meloidogyne incognita densities by 87% and 68% in the field, and 86% and 40% in pots, respectively, which led to a 3.3- and 3.7-fold increase in yield in the field. This novel technology, also referred to as ‘Wrap & Plant’, presents a practical option for nematode management in potato under the resource-limited conditions of sub-Saharan Africa and offers potential for the targeted management of other soil-borne diseases.
The first record of hyperparasitism in mermithid nematodes
Authors: Fujimori Yuta , Kanzaki Natsumi , Shinya Ryoji 
Published: November 2024

Summary
The Mermithidae are primarily simple parasitoids of insects. In this study, we report the first observation of hyperparasitism by mermithid nematodes. Two Mermithidae individuals emerged from a specimen of the parasitoid wasp Coleocentrus incertus collected in Hokkaido, Japan. Although the nematodes were in the juvenile stage and many morphological characteristics were underdeveloped, we were able to observe cross fibre layers on the cuticles. A molecular sequence analysis of the 18S ribosomal DNA suggested that the nematode belonged to the genus Hexamermis, which was supported by the presence of the cross fibre layers. The phylogenetic analysis also suggested a close relationship between our specimens and ‘Mermithidae sp. NM1’, a taxon found in bumblebees, as differences between the sequences of the two taxa were minimal; however, further research is required to confirm the species’ identity. Hyperparasitism by nematodes is rare and is typically unsuccessful in non-host species, yet the nematodes in this study successfully parasitised a parasitoid wasp. Our study is the first to demonstrate that mermithid nematodes can exhibit hyperparasitism in addition to their known parasitic behaviour. The parasitic strategies of Mermithidae nematodes appear to be more flexible than previously assumed, indicating a high degree of adaptability as parasites.
Silver Gorgoneion from the Cult Place at Eklizi-Burun (Mountain Crimea)
Authors: Lysenko Aleksandr , Mordvintseva Valentina 
Published: November 2024

Abstract
During a survey on the territory of the Eklizi-Burun cult complex, a repeatedly densely folded fragment of a silver sheet was discovered. When unfolded during the restoration process, it turned out to be part of an art object depicting the mask of Medusa Gorgon. The size and shape of the item suggest its purpose as part of Roman military equipment (presumably part of the umbo or a knee guard). The stylistic features of the image give grounds to attribute it to the late Roman period.
Notes on a Glass Assemblage and Associated Wick-Holders from Machkhomeri (Khobi Municipality – Samegrelo-Zemo Svaneti, Western Georgia)
Authors: Intagliata Emanuele E. 
Published: November 2024

Abstract
The 2018–2019 excavation of the basilica church at Machkhomeri yielded a rich assemblage of glass vessels, most of which are believed to belong to 6th century AD lamps. These were dumped into an annexed funerary chapel when the church was restructured to become a single-hall building. Glass lamps are well-known occurrences in late antique contexts across the Mediterranean and the Black Sea. However, the finds from the southwest Caucasus have often been neglected. The scope of this contribution is to present the glass finds from Machkhomeri. A collection of wick-holders for glass lamps retrieved in close stratigraphic proximity to the glass assemblage will also be described.
Towards an Archaeological Appraisal of the Central Zagros in the Achaemenid Period
Authors: Bahadori Ali 
Published: November 2024

Abstract
Located in the great satrapy of Media, during the Achaemenid period central Zagros was a crucial region throughout this era in western Iran, but archaeologically it has not so far been analyzed because of lacking material evidence. However, a putative reconstruction may be presented on the basis of current documentation. On this ground and as historical geography interpretations suggest, the central, southern and eastern parts of this region were more important, while the western and northern parts are still waiting for further explorations. Ecbatana, Nush-i Jan and Godin formed most significant centers in the eastern part, but the present paper argues that it was Bisotun in the central part that echoed Achaemenid power on various administrative, economic, military and ceremonial levels.
Détourner un héritage stylistique. Du bon usage des stylèmes galants dans l’Histoire d’une Grecque moderne de Prévost d’Exiles
Authors: de Courson Clara 
Published: November 2024

Résumé
Dans l’Histoire d’une Grecque moderne de Prévost (1740), plusieurs traits de style apparentent l’écriture romanesque à la galanterie littéraire, emblématisée au siècle précédent par Racine ou Lafayette ; c’est le cas en particulier de l’association à l’article indéfini des expressions qui désignent le personnage féminin. Trait de genre (la nouvelle historique et galante), mais aussi d’époque (le long XVIIe siècle), ce tour devient sous la plume de Prévost un marqueur de son style d’auteur. Toutefois, sous l’apparence de la continuité, la valeur et le sens de ce legs stylistique se voient reconfigurés de fond en comble : Prévost orchestre un véritable détournement du style galant. En récupérant l’un des traits par lesquels la langue galante signalait sa littérarité et en l’accommodant à ses propres enjeux romanesques, au risque d’en modifier la signification en profondeur, Prévost offre un cas d’école pour s’interroger sur la propriété des faits de style.
Fascism, Anti-Liberalism and Liberalism in Italy
Authors: Bianchi Alvaro 
Published: November 2024

Abstract
Anti-liberalism is often highlighted as central to interpretations of fascism. This article discusses the existence of a liberal-fascist current in Italian Fascism. Very active during the first years of the Fascist government, this current was expressed in the pages of the journals La Nuova Politica Liberale, which brought together disciples of the philosopher Giovanni Gentile, and Critica Fascista, an organ of the revisionists led by Giuseppe Bottai. Anti-individualist, anti-naturalist and anti-democratic, this current asserted that the rights of the nation preceded individual rights, that freedom would not exist in a supposed state of nature and would be the creation of a political society. It rejected the opposition between freedom and authority, stating that only a strong and orderly state would allow freedom to occur. Although the existence of a liberal-fascist current does not impugn the predominantly anti-liberal character of Italian Fascism, bringing it to light contributes to a more complex assessment of Italian Fascism, underlining the existence of different intellectual currents, ideological clashes, and political antagonism within the movement.
Saumyendranath Tagore and the Nazi Seizure of Power
Authors: Ortiz Michael 
Published: November 2024

Abstract
This article examines the Nazi seizure of power from the perspective of Indian communist Saumyendranath Tagore (1901–1974). In the Weimar era, Berlin developed into a hub of transnational anti-colonial activism. After seizing power, the Nazis forcibly dismantled these networks and terrorized several leading members of the Indian diasporic community. In April 1933, Tagore was arrested for allegedly planning the assassination of Hitler. After fleeing Berlin, Tagore publicly urged progressive forces from around the world to confront the Nazi menace. Like many global anti-fascists, he argued that anti-colonialism encompassed anti-Nazism (as well as vice-versa). Hitler, Tagore argued, could not fathom the emancipation of India, nor the collapse of European imperial hegemony. He would sooner preside over a German Raj than support the self-determination of India.
‘Wake Up—John’
Authors: Jackson Paul 
Published: November 2024

Abstract
This article examines the published literatures of the British proto-fascist group the British Fascists (BF). It argues that, while failing to develop a ‘fully-fledged’ fascist ideology, the organisation’s materials fostered an emotionology, or instructions on how to think and feel, that contained some fascist qualities, especially profound anxieties over the nation facing an existential threat. It draws on conceptual framings from the history of emotions to assess how the BF promoted a variegated range of views, united by deep worry and fear over Communism and profound concern over its potential to influence the future of Britain and its empire, as well as corrupting a future generation through influencing the minds of children. While the BF’s solutions often lacked the fully palingenetic vision typical of fascists, the BF helped to foster a fascist-style emotional refuge and developed this through ideological instruction as well as providing social opportunities and in principle empowerment. Finally, it concludes that the study of such emotionologies allows for deeper consideration of the overlapping political spaces of the right and processes of fascistisation.
Fascism in Peru
Authors: Gayozzo Piero 
Published: November 2024

Abstract
Contemporary fascism is an understudied phenomenon in Latin America. As a contribution to this understudied area, this article analyzes the evolution of fascist thought in Peru from its interwar history to the twenty-first century. Following Roger Griffin’s influential ‘new consensus’ approach to fascism studies based on a fascist minimum, this study explores Peruvian fascism and will argue that fascism developed new expressions in the years after 1945. It will develop the terms ‘neo-fascism’ and ‘post-fascism’, based on Griffin’s fascist minimum, to clarify the nature of the fascist movements to be analyzed. It will conclude that historically Peruvian fascism was exemplified by the Partido Unión Revolucionaria [Revolutionary Union Party], while in the postwar period, it took two parallel directions: the uniquely Peruvian neo-fascism of Acción Legionaria [Legionary Action] and the ethnocacerist or Andean post-fascism of Antauro Humala.
Determination of microplastics in reared black soldier fly larvae (Hermetia illucens) using polarised light optical microscopy
Authors: Dam N.L. , van der Borg G. , Hosseini A. , van Raamsdonk L.W.D. , Zheng R. , Schmitt E. , Hedemann J.B.G.M. , Ruis S. , van Bemmel G. , Meijer N. 
Published: November 2024

Abstract
Microplastics have become ubiquitous in the environment and are increasingly found in a variety of matrices, including in animal feed. At the same time, the presence of plastics from packaging materials in animal feed is still prohibited in the European Union. This is a major barrier preventing certain organic waste streams from being repurposed as feed. However, issues associated with the presence of plastic particles in feed for ‘conventional’ livestock such as poultry and pigs (e.g. accumulation in stomach/other tissues) may not pose a problem to reared insects such as black soldier fly larvae (BSFL). In the hypothetical absence of such transfer, insects reared on feed contaminated with plastics could potentially be repurposed as feed. Such reuse would be a major opportunity for a more circular food chain. The aim of this exploratory study was to develop a method to determine the potential transfer of microplastics from the substrate to biomass of reared BSFL. Tested plastics consisted of low-density polyethylene (LDPE) of three different sizes, and polyethylene terephthalate (PET); to mimic the types of synthetic polymers commonly used for food packaging. The results showed no significant effects of dietary exposure of tested (micro-)plastics on larval yield and survival. We benchmarked fluorescence microscopy through Nile Red staining against a newly developed technique utilizing polarised light optical microscopy to identify plastic particles in BSFL. Tested LDPE particles were virtually absent from the post-exposure larvae. PET was recovered from the larvae, although it is unclear what the exact mechanism for this transfer was. More research is needed to validate these experimental findings with plastics of different types and particle sizes.