bluuebunny/crossref_metadata_2025_split huggingface dataset:
BLADDER RECONSTRUCTION DURING SEPARATION OF A CONJOINT TWIN ISCHIOPAGUS TRIPUS
Authors: Effendi Fajar , Siregar Safendra , Sibarani Jupiter 
Published: August 2019

Objective: Conjoined twins represent one of the rarest congenital anomalies occurring with a varying incidence of about approximately 1 : 50.000 - 1 : 200.000 births. Ischiopagus conjoined twinning (Fuse at the hip region) is even rare representing only 6% of all conjoined twins. Here we report our experience in lower urinary tract reconstruction during separation of a conjoined twin ischiopagus tripus in Hasan Sadikin Hospital, Bandung. Case(s) Presentation: Two years old female conjoined twins, weighing in total of 8 kilograms, were brought to our hospital. The twins were fused at the lower halves of their bodies with two separate lower limbs at the right side and a fused left lower limb. External genitalia were absent. From CT Angiography we found four kidneys, two in each child with two separate bladders, with right ureter of baby A and left ureter of baby B enter the right bladder, while left ureter of baby A and right ureter of baby B enter the left bladder. There was one cloaca that drains urine and feces. During the separation surgery, we performed ureteroneocystostomy and temporary ureteral stenting, followed by cystostomy. The pediatric surgeon performed bowel separation by stapling technique, pelvic wall separation, ileostomy, and pelvic floor reconstruction. The orthopedic surgeon performed femur separation, pelvic floor reconstruction, reconstruction of the pelvic ring, and femur reconstruction. The patients underwent defect closure, which performed by plastic surgeon. The operation was successful, without any complication to the kidney and the urinary tract of both babies. Discussion: Ischiopagus conjoined twins are the most complex conjoined twins and occur in only 6% of all conjoined twin cases. Ischiopagus is usually located along the axis with the head located on the opposite side. This case usually has one umbilicus and the two bodies are fused below this level, the union occurs in the lower abdomen and the pelvis. The internal organs that usually coalesce in cases of ischiopagus conjoined twins are liver, lower gastrointestinal, and genitourinary. The fused gastrointestinal part is the ileo-colic part of terminal ileum (Meckel's diverticulum). Conclusion: The separation of conjoined twin is a unique challenge due to its complex anatomy and physiology. Although advancement in imaging and monitoring has improved the survival rate separation can be successfully achieved only with meticulous planning and team work.
FIRST EXPERIENCE IN VIDEOURODYNAMIC STUDY AT CIPTO MANGUNKUSUMO HOSPITAL IN 2012-2014
Authors: Tambunan Nicholas Albert , Rahardjo Harrina E 
Published: August 2019

Objective: Accuracy of diagnosis is a fundamental thing in terms of plan of treatment. Nowadays, symptoms from lower urinary tract can be diagnosed accurately with videourodynamic study (VUDS). Videourodynamic combines a fluoroscopic voiding cystourethrogram with multichannel urodynamics, allowing anatomic and functional assessment of the bladder and outlet. Material & Methods: This research is a descriptive retrospective. Data was taken from the patients who underwent VUDS at Department of Urology Cipto Mangunkusumo Hospital from 2012 to 2014. Patient’s characteristics were gender, age, chief complaint, working diagnosis pre investigation, and diagnosis after VUDS was performed. Then, data was analyzed in a descriptive fashion and presented using tables and narative form. Results: There were 8 male patients (67%) and 4 female (33%) who underwent VUDS. Most of the patients were children (75%). Five patients (42%) complained about incontinence with or without other LUTS, only 1 patient (8%) had LUTS and disuria. Overall, working diagnosis pre investigation was neurogenic bladder with various causes. We found a variable diagnoses after VUDS. At filling phase, we found 7 patients (58%) with small bladder capacity, 5 patients (42%) with low bladder compliance then followed respectively by overflow incontinence (33%), detrusor overactivity (17%) and urethral sphincter incompetence (8%). At voiding phase, the most common abnormality detected were detrusor underactivity (42%), followed by infravesical obstruction (8%). VUR was found both at filling phase (42%) and voiding phase (33%). Conclusion: VUDS could be the first choice of modality for diagnosing dysfunction of lower and upper urinary tract especially in neurogenic cases.
RISK FACTORS OF MORTALITY IN CHILDREN WITH WILMS’ TUMOR AT SOETOMO HOSPITAL SURABAYA
Authors: Shanty Maria Christina , Yuniarchan Sherly , Andarsini Mia Ratwita , Ugrasena I Dewa Gede , Permono Bambang , Prasetyo Risky Vitria 
Published: August 2019

Objective: Wilms’ tumor is the most common childhood renal tumor for about 6% of pediatric malignant disease. The 5-year survival rate in United States increased from approximately from 70% (1970-1973) to 92% (1989-1996). This study was aim to analyze the risk factors of mortality in children with Wilms’ tumor. Material & Methods: A cross-sectional study was conducted in children with Wilms’ tumor at Soetomo Hospital during 2006-2011. The data of demographic, clinical profile, complete blood count, blood urea nitrogen, glomerular filtration rate, histological type, disease stage, metastases and relapse were analyzed as risk factors of mortality using logistic regression. Results: There were 37 Wilms’ tumor children and 5 children were excluded because of incomplete data. The mean age was 3.0 (SD 2.6) years, and male-to-female ratio was 2.5 : 1. There were 5/32 children in stage I, 7/32 children in stage II, 8/32 children in stage III, 11/32 children in stage IV, and 1/32 children in stage V. There were 15/32 children underwent operation. Complete remission occurred in 12/32 children and 1/32 children relapsed. There were 20/32 children died, associated with anemia (P=0.033, OR=6.111, 95% CI=1.056-35.352) and advanced stage (P=0.021, OR=8.000, 95% CI=1.575-40.632). The risk of mortality increased 3.284 folds with every increased stage (P=0.007, 95% CI=1.338-7.775). Conclusion: Disease stage is the significant risk factor of mortality in children with Wilms’ tumor.
THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENS
Authors: Pramanta Aditya , Renaldo Johan , Soebadi Doddy M 
Published: August 2019

Objective: The patency rates after vasectomy reversal ranges from 71-97%, but there is 26-72% possibility of persistent infertility. Dysfunction or obstruction of the epididymis and oxidative stress are thought to be the important cause of male infertility by disrupting spermatozoa maturation process, causing poor sperm quality. Human erythropoietin or better known as EPO is a glycoprotein hormone that has been purified since three decades ago. Research on the EPO has evolved and become a major research topic the researchers aimed as a therapeutic agent. The cloning and expression of erythropoietin has developed recombinant erythropoietin as a drug that serves as an anti-oxidant, anti-apoptotic and anti-inflammatory. This study aimed to determine the effect of erythropoietin supplementation on sperm motility and morphology in Wistar rat after the release of the vas deferens’ ligation. Material & Methods: Twenty four male Wistar rats were randomly divided into four groups (6 each). On the vasectomy group, the vas deferens were serially ligated for 7 weeks using a non-absorbable suture. The vasectomy reversal group get the same surgical treatment and after 7 weeks the ligation were released. While as in the erythropoietin group, recombinant eryhtropoietin (1000 IU/kg) was administered intraperitoneally three times for 1 week after releasing the ligation. Normal control animals received no surgical manipulation and followed by sperm retrieval for analysis. Eosin-stained slides were prepared to assess the motility and morphology of sperm cells and observed under a light microscope. Results: Ligation of vas deferens significantly decreased sperm motility and morphology. Releasing the ligation of the vas deferens did not improve the sperm motility and morphology. Supplementing eryhtropoietin 1000 IU/kg 3 times for a week after releasing the ligation did not improve the sperm motility and morphology. Conclusion: Erythropoietin supplementation did not improve the sperm motility and morphology in Wistar rat after releasing the ligation of vas deferens.
THE EFFECTIVENESS OF TAMSULOSIN, SOLIFENACIN, AND COMBINATIONS THERAPY TAMSULOSIN ADDED SOLIFENACIN ON LOWER URINARY TRACT SYMPTOMS AFTER DOUBLE J STENT INSERTION
Authors: Pramana Ida Bagus Putra , Rizaldi Fikri , Djojodimedjo Tarmono 
Published: August 2019

Objective: Insertion of DJ Stent is a procedure that is often done by urologist. Insertion of DJ Stent can cause LUTS complaints and greatly affect the physical and psychosocial health of patients. The aim of this study was to determine the effectiveness difference of tamsulosin therapy 0.4 mg/day, solifenacin 5 mg/day and the combination of tamsulosin therapy 0.4 mg/day added solifenacin 5 mg/day to manage LUTS complaint after DJ Stent insertion. Material & Methods: This study was a randomized placebo-controlled trial. There were 4 groups, group I received placebo, group II received tamsulosin 0.4 mg/day, group III received solifenacin 5 mg/day, and group IV received combination therapy of tamsulosin 0.4 mg/day added solifenacin 5 mg/day. Evaluation based on International Prostatic Symptom Score (IPSS) and Ureteral Stent Symptom Questioner (USSQ) score. Data were analyzed using SPSS 21.0. It is said to be significant if p<0.05. Results: There were a total of 32 samples consist of 19 (59.3%) men and 13 (40.6%) women. There were significant improvements in the score of total IPSS, IPSS storage and IPSS quality of life score in patients who received combination therapy (p<0.05) when compared with patients who received monotherapy. The highest decrease in USSQ scores 1, 2, 3, 4, 5 and 6 were in the group that received combination therapy when compared with the monotherapy group. Conclusion: The combination therapy is safe and effective to improve IPSS total, IPSS storage and IPSS Quality of Life scores compared with monotherapy.
COMPARISON BETWEEN TRANSRECTAL ULTRASONOGRAPHY GUIDED TRANSRECTAL PROSTATE BIOPSY AND TRANSRECTAL ULTRASONOGRAPHY GUIDED TRANSPERINEAL PROSTATE BIOPSY
Authors: Halfian Randa , Soebadi Doddy M , Rizaldi Fikri 
Published: August 2019

Objective: Prostate cancer is the fourth most common malignancy in men. TRUS guided transperineal prostate biopsy and TRUS guided transrectal prostate biopsy are two main approach to take prostate tissue as diagnostic of prostate cancer. To compare prostate biopsy approach between TRUS guided transrectal and TRUS guided transperineal toward duration of examination, pain perception, and complications. Material & Method: This study was an experimental study with prospective approach. There were two groups, group one was performed TRUS guided transrectal prostate biopsy (TRB) and group two was performed TRUS guided transperineal prostate biopsy (TPB). Evaluation was based on the duration of examination, pain perception, and complication. Data was analyzed using independent T test for duration of examination and Mann-Whitney test for pain perception. Data was performed using SPSS 21.0 version. The statistical significant difference was consider if p value <0.05. Results: There were 20 samples in this study. There was a significant difference in the duration of examination, the average duration of TPB examination (17.40 ± 2.50) was longer than the duration of TRB examination (14.1 ± 2.77). There was no significant statistical difference between TPB group and TRB group in pain perception when USG probe into the anal (p=0.65), anesthesia process (p=0.28), prostate tissue sampling (p=1.00), and post biopsy (p=0.34). Rectal bleeding was found mostly in TRB group (40%) compared to TPB group (0%). Hematuria was experienced by three patients (30%) in TRB group and two patients (20%) in TPB group. Conclusion: TRB was more effective in duration of biopsy than TPB. The complications of rectal bleeding and hematuria were more in TRB group than TPB. The pain perception were the same between both groups. There were no fever, sepsis, hematospermia and vasovagal event in two groups.
THE EFFECT OF NIFEDIPINE ON SURVIVE CELL, APOPTOTIC CELL AND NECROTIC CELL OF IPSILATERAL TESTICULAR GERMINAL EPITELIAL CELLS ON MALE WISTAR RATS WITH UNILATERAL TESTICULAR TORSION
Authors: Kurniawan Andrie Rhomdhon , Hakim Lukman , Soebadi Doddy M 
Published: August 2019

Objective: This study aimed to compare the number of survival, apoptotic and necrotic cells of ipsilateral testicular germinal epithelial cells in male wistar rats with unilateral testicular torsion between nifedipine given and control groups. Material & Methods: Thirty male wistar rats aged 10-12 weeks were randomly divided into 5 groups, each consisted of 6 rats. The negative control group (KN) underwent a sham procedure and left orchidectomy. Positive control group 4 (KP4) and 10 (KP10) performed left torsio testis 3 x 360 degrees medially for 4 hours and 10 hours respectively, then performed orchidectomy 4 hours after detorsion. The 4-hour (N4) and 10 hours (N10) nifedipine treatment group received the same treatment with positive control, but 30 min before detorsion performed, nifedipine were given intraperitoneal 100μg/kg. Within 1 hour after orchidectomy, cell count was calculated using flow cytometry. Results: It was found that the 4 (N4) and 10 hours (N10) nifedipine treatment group had a higher survival cells and also a lower number of apoptotic and necrotic cells compared to the positive control group. It was found that the 10 hours nifedipine treatment group (N10) had a lower number of apoptotic and necrotic cells compared to the 10 hour positive control group (KP10). The difference was statistically significant with p value <0.05. However, in KP4 and N4 group compared with KP10 and N10 group, higher apoptotic cells was obtained. This was a new phenomenon that needs to be investigated more deeply. Conclusion: Intraperitoneal administration of nifedipine prior to testicular detorsion may reduce the number of apoptotic and necrotic cells of testicular germinal epithelial cell, and may increase the number of survival cells in ipsilateral testes with unilateral testicular torsion.
THE EFFECTIVITY OF EUTECTIC MIXTURE OF LOCAL ANESTHETICS CREAM AS EXTRACORPOREAL SHOCKWAVE LITHOTRIPSY ADJUVANT ANALGESIA IN SOETOMO GENERAL HOSPITAL
Authors: Sholihin Riza Mazidu , Hakim Lukman , M. Soebadi Doddy 
Published: August 2019

Objective: The Extracorporeal Shockwave Lithotripsy (ESWL) procedure is the first choice for kidney stone therapy with the size ≤20mm. One of the complications of ESWL is the pain. Pain control during ESWL is essential to maintain the focus point of ESWL and give enough time to achieve complete stone fragmentation. Until now there is still no data about the effectiveness of Eutectic Mixture of Local Anesthetics (EMLA) cream as an adjuvant analgesic for ESWL procedure in Soetomo Hospital Surabaya. To compare the onset of pain and ESWL Wong-Baker Pain Scale (WBPS) between the EMLA group and placebo group. Material & Methods: The design of this study was a single-blind randomized control trial comparing the onset of pain and Wong Baker pain scale between the patient in EMLA group and placebo one. WBPS is assessed in minute 15, 30, 45, and 60 of ESWL sessions and 60 minutes post ESWL procedure. The results were analyzed with SPSS. Pain onset and differences of WBPS were tested by using independent T-test if the data is normally distributed and using Mann-Whitney test if the data is not normally distributed. Results: 10 patients were obtained in each group. The mean onset of pain in the EMLA group was longer than the mean onset of pain in the placebo group but statistically not significant (p>0.05). The WBPS in the EMLA group was significantly lower compared with the placebo group in the 30, 45, and 60 minutes of ESWL (p<0.05). Conclusion: EMLA cream application before ESWL was effective and safe as an adjuvant analgesic to reduce pain during ESWL.
THE EFFECTS OF NICOTINE EXPOSURE PER INHALATION TO THE CHANGE OF MOTILITY AND MORPHOLOGY OF THE RAT’S SPERM
Authors: Nugroho Puruhito Eko , Rizaldi Fikri , Soesanto Wahjoe Djati 
Published: August 2019

Objective: Smoking are known to have detrimental effect on sperm. The effects are suspiciously come from nicotine as its main pharmacologic component. This study is carried out to analyze the effect of nicotine exposure per inhalation to the difference of motile sperm percentage and normal morphology sperm percentage of male wistar rats. Material & Methods: Thirty male rats were used for this research. They were randomly divided into 5 groups and treated with nicotine inhalation 0.5 mg/kgBW, 1 mg/kgBW, 2 mg/kgBW and 4 mg/kgBW for 30 days while the control rats were not given any treatment. At the end of experiment, sperm analysis was carried out to measure the percentage of motile sperm and percentage of sperm with normal morphology. Results: Percentage of motile sperm significantly decreased (p<0.05) in all treatment groups compared to control group. Comparison between treatment groups revealed that the percentage of motile sperm significantly decreased in 0.5 mg/kgBW vs 2 mg/kgBW, 0.5 mg/kgBW vs 4 mg/kgBW, and 1 mg/kgBW vs 4 mg/kgBW. Percentage of normal morphology sperm decreased significantly (p<0.05) in 1 mg/kgBW, 2 mg/kgBW, and 4 mg/kgBW compared to control groups. Comparison between treatment groups revealed that there were no significant decreased between each treatment groups. Conclusion: This research conclude that nicotine exposure per inhalation can deteriorate the percentage of motile sperm and the percentage of normal morphology sperm of male wistar rats.
Isolation and Identification of Pesticides Degrading Bacteria from Farmland Soil
Authors: Rahman Md Atikur , Arefin ASM Shamsul , Saha Otun , Rahaman Md Mizanur 
Published: August 2019

Pesticides are recognized to be the threat to the environment and associated with a wide range of serious diseases including respiratory diseases, cancer and even birth defects. In this study, six-different bacterial strains capable of degrading Carbofuran, Emamectin Benzoate and Thiamethoxam were isolated from eight different soil samples. The isolates were characterized by using different conventional and molecular methods. The strains were identified molecularly into different genotypes using amplified ribosomal DNA restriction analysis (ARDRA) and partial sequencing of 16S rDNA. The ARDRA pattern clustered them into 3 groups. Among the isolates three were identified as Achromobacter spp. and one as Diaphorobacter sp. by biochemical tests. It was further confirmed by the partial 16S rDNA sequencing. The two identified potential bacteria can be used for biodegradation of different pesticides which can have a significant environmental impact in soil farm.
Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 90-94
Seasonal variation of bacterial load in river and lake waters of Dhaka city, Bangladesh
Authors: Sikdar Md Suprio Sadat , Abony Maruf , Zerin Tamanna , Banik Avijit , Datta Suvamoy 
Published: August 2019

Surface water is often contaminated due to human excreta and urban and industrial pollution. The increased population growth and industrialization are likely to exacerbate the situation. Microbiological analysis of waters from rivers (Turag and Buriganga) and lakes (Banani and Dhanmondi) around Dhaka city were conducted for Spring, Monsoon and Fall seasons of the year, 2016 and 2017. Total Viable Count (TVC), Total Coliform Count (TCC), Total Fecal Coliform Count (TFCC) and Total Salmonella Shigella Count (TSSC) were conducted to obtain bacterial load in both enriched and without enrichment water samples. All the Isolates were identified by microscopy, cultural characteristics and biochemical methods. Most of the water reservoirs have a decline in the bacterial load in monsoon where the microbial load of pathogens is highest during spring. Without enrichment, E. coli, Enterobacter sp., Klebsiella sp. and S. aureus were abundant, where others like Shigella sp., Proteus sp., Serratia sp., Pseudomonas sp., Acinetobacter sp., and Alcaligenes faecalis mostly exhibited growth following enrichment. Results indicate that pathogenic environmental isolates can cause serious health issue if water is left untreated or poorly treated from reservoirs within and around Dhaka city.
Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 116-121
The Microbiological Quality of Commercial Fruit Juices-Current perspectives
Authors: Ahmed Tasnia , Das Kamal Kanta , Uddin Md Aftab 
Published: August 2019

The purpose of this review article is to introduce the importance of fruit juices for human health living in the country and in a broad, and to develop awareness among the people about the diseases caused by pathogens associated with fruit juice. Health benefits of juices have been included in this article and how the same juice can cause problems among people of different ages have been discussed. Contamination sources and the ways to prevent them is very important issue in protecting public health Some future recommendations for fruit juices have also been added in this article. Different diseases caused by various microbial agents and the associated symptoms after consuming contaminated fruit juices worldwide are discussed in this review. This review was aimed at the possible sources of microbial contamination, disease caused by them and determining some ways to avoid such phenomena. From the information provided here, it was noticeable that commercial fruit juice can also harbor pathogenic microorganisms which can cause serious disease outbreaks. The contamination can also be initiated during in house consumption if lack of awareness prevails among the consumers. Manufacturing process should be much more strict in this regard to assure the public health safety. Commercially available fruit juices are consumed worldwide among different ages of people and if not processed properly, this healthy drink may be hazardous for human health.
Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 128-133
Adaptation of environment friendly technology by reducing pesticide use for cucumber cultivation at Farmers’ level
Authors: Alam MAU , Hossain M , Mia MI , Talukder MAAH , Haque MMU , Khandakar J 
Published: August 2019

A field experiment was conducted in the farmer’s field at Salaipur, Mithapukur, On Farm Research Division, Rangpur during 2015-2016to control cucurbit fruit fly effectively through the use of sex pheromone trap. The trial was conducted among six farmers. Three treatments viz., T1 (Sex pheromone + Poison bait + Sanitation (9 spot)), T2 (Sex pheromone + Poison bait + Sanitation (16 spot)) and T3 (Farmers practice) were compared. The total land area was 7200m2. The higher fruit yield (63.99t/ha) was obtained from T2 (Sex pheromone + Poison bait + Sanitation (16 spot). The lower fruit yield (27.99 t/ha) was obtained from T3. The gross margin (Tk. 294706/ha) and gross return was also higher with the use of T2 (Sex pheromone + Poison bait + Sanitation (16 spot).The highest gross margin and gross return was found in T2 (Sex pheromone + Poison bait + Sanitation (16 spot) treatment. Use of insect lures can improve safe vegetable production as well as yield. Innovative agricultural technologies have the potential to increase yield and income of smallholder vegetable farmers in the northern region of Bangladesh.
Progressive Agriculture 30 (2): 150-156, 2019