bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cablinosides A and B, Two Glycosidic Phenylacetic Acid Derivatives from the Leaves of Pogostemon cablin
Authors: Huang Xiao‐Jun , Li Ping , Yin Zhi‐Qi , Lu Jin‐Jian , Lin Li‐Gen , Wang Ying , Ye Wen‐Cai , Zhang Qing‐Wen 
Published: April 2019

AbstractA pair of new glycosidic epimers, cablinosides A (1a) and B (1b) were isolated from the leaves of Pogostemon cablin. The structures with absolute configurations of 1a and 1b were elucidated by extensive NMR investigation, and quantum chemical CD calculations. The epimer mixture 1 showed moderate α‐glucosidase inhibitory activity and no significant cytotoxic activity against HepG2 cells.
Near‐Infrared Bioluminescence Imaging with a through‐Bond Energy Transfer Cassette
Authors: Abe Masahiro , Nishihara Ryo , Ikeda Yuma , Nakajima Takahiro , Sato Moritoshi , Iwasawa Naoko , Nishiyama Shigeru , Paulmurugan Ramasamy , Citterio Daniel , Kim Sung Bae , Suzuki Koji 
Published: April 2019

AbstractA coelenterazine (CTZ) analogue emitting near‐infrared (NIR) bioluminescence was synthesized for through‐bond energy transfer (TBET)‐based imaging modalities. The analogue, named Cy5‐CTZ, was prepared by conjugating cyanine‐5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR‐shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5‐CTZ substrate is optically stable in physiological samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells.
Intracellular and Cellular Detection by SERS‐Active Plasmonic Nanostructures
Authors: Wu Di , Chen Yonghao , Hou Shuai , Fang Wenjun , Duan Hongwei 
Published: April 2019

AbstractSurface‐enhanced Raman scattering (SERS), with greatly amplified fingerprint spectra, holds great promise in biochemical and biomedical research. In particular, the possibility of exciting a library of SERS probes and differentially detecting them simultaneously has stimulated widespread interest in multiplexed biodetection. Herein, recent progress in developing SERS‐active plasmonic nanostructures for cellular and intracellular detection is summarized. The development of nanosensors with tailored plasmonic and multifunctional properties for profiling molecular and pathological processes is highlighted. Future challenges towards the routine use of SERS technology in quantitative bioanalysis and clinical diagnostics are further discussed.
Regulation of insulin resistance by targeting the insulin‐like growth factor 1 receptor with microRNA‐122‐5p in hepatic cells
Authors: Dong Li , Hou Xiaoyu , Liu Fengsui , Tao Hong , Zhang Yunjia , Zhao Hang , Song Guangyao 
Published: April 2019

AbstractInsulin resistance (IR) is a common etiology of type 2 diabetes (T2D) defined by a state of decreased reactivity to insulin in multiple organs, such as the liver. This study aims to investigate how microRNA‐122‐5p (miR‐122) regulates the hepatic IR in vitro. We first found that the miR‐122 level was upregulated in the liver of rats fed with a high‐fat diet and injected with streptozotocin (T2D rats), while the expression level of insulin‐like growth factor 1 receptor (IGF‐1R), a potential target of miR‐122, was downregulated in the diabetic liver. In vitro, glucosamine‐induced IR was introduced in HepG2 hepatic cells, and the levels of miR‐122 and IGF‐1R were further assessed. An increase of miR‐122 level and a decrease of IGF‐IR level were observed in IR hepatic cells, which was the same as that in the diabetic liver. Results of the luciferase reporter assay validated IGF‐1R as a direct target of miR‐122. Moreover, in IR HepG2 cells, antagonizing miR‐122 with its specific inhibitor enhanced glucose uptake and suppressed the expression of glucose 6‐phosphatase and phosphoenolpyruvate carboxykinase, two key enzymes in regulating gluconeogenesis. Such alterations induced by the miR‐122 inhibitor in IR hepatic cells were impaired when IGF‐1R was simultaneously knocked down. In addition, the PI3K/Akt pathway was deactivated in IR cells, and then reactivated with miR‐122 inhibitor transfection. In conclusion, our study demonstrates that miR‐122 is able to regulate IR in hepatic cells by targeting IGF‐1R.
Let‐7b‐5p inhibits proliferation and motility in squamous cell carcinoma cells through negative modulation of KIAA1377
Authors: Zheng Shutao , Liu Qing , Ma Rong , Tan Doudou , Shen Tongxue , Zhang Xiao , Lu Xiaomei 
Published: April 2019

AbstractKIAA1377 has been found to be linked with lymph node metastasis in esophageal squamous cell carcinoma (SCC) in our previous study; however, the regulation of KIAA1377 remains far from understood. Herein, to understand the regulation of KIAA1377 from the angle of microRNA (miRNA)–messenger RNA (mRNA) modulation in the setting of SCC cells, the basal level of KIAA1377 was determined by quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blot analysis in KYSE‐150 and HeLa cells; biological roles of KIAA1377 contributing in the proliferation, migration, and invasion were evaluated using 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT), wound‐healing and Transwell assays, respectively, after KIAA1377 was knocked out mediated by the CRISPR‐Cas9 system. Bioinformatic prediction revealed that let‐7b‐5p was a putative miRNA regulating KIAA1377, which was ensuingly validated by the luciferase reporter assay; after which, variation of KIAA1377 expression was further verified by qRT‐PCR and western blot analysis. Moreover, the biological roles of let‐7b‐5p in proliferation, migration, and invasion of KYSE‐150 and HeLa cells were also evaluated. It was exhibited that KIAA1377 was able to promote the proliferation and motility of both KYSE‐150 and HeLa cells, which can be reverted by re‐expression of let‐7b‐5p. The luciferase reporter assay verified that let‐7b‐5p can diametrically target KIAA1377. Collectively, our data demonstrated that let‐7b‐5p can directly but negatively regulate KIAA1377 in SCC cell lines, Ecal109, and HeLa cells.
JQ1, a BET‐bromodomain inhibitor, inhibits human cancer growth and suppresses PD‐L1 expression
Authors: Liu Kaisheng , Zhou Zhifan , Gao Hengyuan , Yang Fang , Qian Yajun , Jin Hongtao , Guo Yaomin , Liu Ying , Li Haili , Zhang Cheng , Guo Jinan , Wan Yong , Chen Rui 
Published: April 2019

AbstractMost traditional cytotoxic chemotherapeutic agents have poor aqueous solubility and significant toxicity. Hence, there is a need to develop molecule‐targeted drugs. Programmed death‐ligand 1 (PD‐L1) is associated with the prognosis of several cancer types, and blockade of PD‐1/PD‐L1 signaling increases the amplitude of anti‐tumor immunity. In the present study, we investigated the effects of JQ1, a bromodomain and extraterminal‐bromodomain inhibitor, on cell growth, and messenger RNA (mRNA) and protein levels of PD‐L1 in renal cell carcinoma primary culture cells, and prostate, liver, and lung cancer cell lines. The results of the cell counting kit‐8 assay suggested that JQ1 inhibits cell growth in a dose‐dependent manner. The mRNA and protein levels of PD‐L1 decreased in the primary culture of JQ1‐treated renal carcinoma, prostate cancer, liver cancer, and lung cancer cell lines. In addition, the mRNA level of PD‐L2 also decreased in the JQ1‐treated cells. Overall, JQ1 might be a potential anti‐tumor agent.
On the interaction of ribosomal protein RPL22e with microtubules
Authors: Chudinova Elena M. , Brodsky Ilya B. , Nadezhdina Elena S. 
Published: April 2019

AbstractMicrotubule (MT) protein preparations often contain components of the translation machinery, including ribosome proteins. To understand the biological meaning of it we studied the interaction of ribosomal protein RPL22e with the MT. We found that bacteria expressed purified RPL22e‐GFP‐6His did co‐sediment with brain tubulin MTs with 1.3 µM dissociation coefficient. Such a KD is comparable to some specific MT‐associated proteins. Distinct in vitro interaction of RPL22e‐GFP with MTs was also observed by TIRF microscopy. In real‐time assay, RPL22e‐GFP molecules stayed bound to MTs for several seconds, and 15% of them demonstrated random‐walk along MTs with diffusion coefficient 0.03 µ2/s. Deletion of basic areas of RPL22e did not have an impact on KD, and deletion of acidic tail slightly increased association with MTs. Interestingly, the deletion of acidic tail increased diffusion coefficient as well. The interaction of RPL22e with MTs is hardly noticeable in vivo in cultured cells, probably since a significant part of the protein is incorporated into the ribosomes. The mobility of ribosomal protein on the MTs probably prevents its interfering with MT‐dependent transport and could ameliorate its transport to the nucleus.
Transition Metal (Fe, Co and Ni)−Carbide−Nitride (M−C−N) Nanocatalysts: Structure and Electrocatalytic Applications
Authors: Jin Jing , Yin Jie , Liu Hongbo , Lu Min , Li Jianyi , Tian Meng , Xi Pinxian 
Published: April 2019

AbstractTransition metal−carbon−nitrogen (M−C−N) nanocrystals, such as hybrid‐structure, heterostructure, and cluster or single metal atoms anchored on carbon−nitrogen skeleton, have attracted wide attention in various fields for their superior physicochemical properties. However, it remains a great challenge to synthesize those M−C−N nanocrystals on a large nanoscale for their intrinsic characteristics, which further limited their extensive application in different fields. In this minireview, we present an overview of those M−C−N nanostructures in terms of their synthetic methods, applications and relationship between structure and properties. In addition, this review will also provide some perspectives on the challenges and opportunities of those nanomaterials.
Al2O3/Yttria‐Stabilized Zirconia Hollow‐Fiber Membrane Incorporated with Iron Oxide for Pb(II) Removal
Authors: Paiman Syafikah Huda , Rahman Mukhlis A , Abas Khairul Hamimah , Abd Aziz Azian , Ismail Ahmad Fauzi , Hafiz Dzarfan Othman Mohd , Jaafar Juhana , Mohd Norddin Mohammad Noorul Anam 
Published: April 2019

AbstractRemoval by absorptive ceramic membranes can simultaneously absorb and separate metal ions from water. Alumina/yttria‐stabilized zirconia (Al2O3/YSZ) hollow‐fiber membranes, fabricated using phase inversion and sintering process, were deposited with iron oxide by an in‐situ hydrothermal process. The results showed that α‐Fe2O3 was produced and incorporated across the membranes. A reduction in flux was recorded with the deposition of α‐Fe2O3. However, it improved the adsorption capacity for heavy metal adsorption. The adsorption‐separation test demonstrated that the optimized membrane is able to completely remove Pb(II) ions after two hours.
A Cost‐Effective and Scaleable Approach for the In‐Situ Synthesis of Porous Carbon‐Coated Micrometer‐Sized AlSi Particles as Anode for Lithium‐Ion Batteries
Authors: Li Cheng , Li Xitao , Zheng Yan‐Zhen , Wu Jiaojiao , Ding Haiyang , Tao Xia 
Published: April 2019

AbstractSilicon is intensively investigated as one of the most promising lithium‐ion battery anode material due to its high theoretical capacity. Here, spherical micro‐sized porous AlSi coated by a carbon layer (AlSi@C) are fabricated from low‐cost commercial AlSi alloy by delicately controlling an acid etching period, followed by polymerization and carbonization. A small amount of residual Al is beneficial to maintain the spherical morphology and enhance the electrical conductivity of the AlSi anode. Profiting from the porous structure and carbon coating layer, micro‐sized porous AlSi@C exhibits a high initial coulombic efficiency of 84.2 %, delivers an initial specific discharge capacity of 1650 mA h g−1 at a current density of 0.50 A g−1, and still retains 824 mA h g−1 after 300 cycles. This work provides a facile and scalable synthetic strategy for the preparation of porous Si‐based anode materials from low‐cost AlSi alloy, and it is expected to realize the industrialization of preparation of porous Si‐based anode materials.
Nanoconfinement of the Low‐Temperature Dark Conglomerate: Structural Control from Focal Conics to Helical Nanofilaments
Authors: Foley Lee , Park Wongi , Yang Minyong , Carlson Eric , Korblova Eva , Yoon Dong Ki , Walba David M. 
Published: April 2019

AbstractThe helical nanofilament (HNF) and low‐temperature dark conglomerate (DC) liquid‐crystal (LC) phases of bent‐core molecules show the same local layer structure but present different bulk morphologies. The DC phase is characterized by the formation of nanoscale toric focal conics, whereas the HNF phase is constructed of bundles of twisted layers. Although the local layer structure is similar in both phases, materials that form these phases tend to form one morphology in preference to the other. Targeted control of the nanostructures would provide pathways to potential applications and insight into how conditions drive a specific phase formation. Here, W624, a compound known to form the DC phase is confined in nanometer scale channels of porous anodized aluminum oxide (AAO) membranes. Within each nanochannel, the DC phase is suppressed forming the HNF structure instead, indicating the nanoscale spatial limitation can control the phase structure of the DC phase.
Batch and Continuous‐Flow Iron(II)‐Catalyzed Synthesis of Sulfilimines and Sulfoximines using N‐Mesyloxycarbamates
Authors: Lai Calvine , Mathieu Gary , Gabrielli Tabarez Lucia Paola , Lebel Hélène 
Published: April 2019

AbstractThe synthesis of sulfilimines and sulfoximines with N‐mesyloxycarbamates in presence of catalytic iron(II) chloride hydrate is described. The use of 1‐butylimidazole as a base proved instrumental in enabling a homogeneous reaction mixture compatible with continuous flow processes. The amination is applicable to a wide range of substrates, and affords the desired product in good to excellent yields within 1 to 60 minutes.
Room‐Temperature Photogeneration of Nitrosyl Linkage Isomers in Ruthenium Nitrosyl Complexes
Authors: Mikhailov Artem A. , Wenger Emmanuel , Kostin Gennadiy A. , Schaniel Dominik 
Published: April 2019

AbstractThe conditions for the photogeneration of NO linkage isomers at room temperature are studied. By pulsed laser irradiation in the blue spectral range, the long‐lived Ru−ON isomer can be generated at room temperature, which is crucial for potential applications, such as holography and data storage. By using static and time‐resolved spectroscopy (UV/Vis and IR), we give evidence that the liftime of the Ru−(η2‐(NO)) isomer is a decisive parameter for the formation of the Ru−ON isomer at high temperature owing to a two‐step isomerization mechanism Ru−NO→Ru−(η2‐(NO))→Ru−ON. Furthermore, we report the low‐temperature structures for each isomer, which were revealed by photocrystallography.
Synthesis of a Perfluorinated Phenoxyphosphorane and Conversion to Its Hexacoordinate Anions
Authors: Marczenko Katherine M. , Johnson Chloe‐Louise , Chitnis Saurabh S. 
Published: April 2019

AbstractWe report the synthesis and structural characterization of a neutral PV Lewis acid, P(OC6F5)5, and salts containing the six‐coordinate anions [P(OC6F5)5F]− and [P(OC6F5)6]−. The latter anion exhibits a rare example of F–πarene interactions in both the solid and the solution phase, which has been quantitatively studied by variable‐temperature (VT) NMR spectroscopy. The Lewis acid strength of P(OC6F5)5 has been assessed through experimental fluoride ion competition experiments and quantum‐chemical calculations of its fluoride ion affinity (FIA) and global electrophilicity index (GEI). Our findings highlight the importance of considering solvent effects in electrophilicity calculations, even when neutral Lewis acids are involved, and show a rare divergence between FIA and GEI trends. The coordinating abilities of the [P(OC6F5)6]− and [P(OC6F5)5F]− anions towards the trityl cation, as a prototypical electrophile, have been assessed.
Preparation of Cells from Embryonic Organs for Single‐Cell RNA Sequencing
Authors: Sekiguchi Rei , Hauser Belinda 
Published: April 2019

AbstractAlthough single‐cell RNA sequencing (scRNA‐seq) has become one of the most powerful methods available for transcriptome analysis, the quality of scRNA‐seq data largely depends on cell preparation. Cell preparation from cultured cells and tissues requires different methods because of the inherent differences between these two categories of cells. Compared to cultured cells, tissues have more extracellular matrix, and the cells are generally more adherent and thus difficult to dissociate. The challenge is to achieve sufficient dissociation, cell counts, and viability all at the same time. This protocol describes approaches that help achieve these goals. These include a cold dissociation technique using cryophilic proteases active at cold temperature, timing of trituration during protease digestion, as well as filtration and washing methods that optimize cell viability and retention. Materials and equipment that optimize the process will also be discussed. © 2019 by John Wiley & Sons, Inc.
Supramolecular Coordination of Pb2+ Defects in Hybrid Lead Halide Perovskite Films Using Truxene Derivatives as Lewis Base Interlayers
Authors: Aktas Ece , Jiménez‐López Jesús , Rodríguez‐Seco Cristina , Pudi Rajesh , Ortuño Manuel A. , López Núria , Palomares Emilio 
Published: April 2019

AbstractTruxene derivatives, due to their molecular structure and properties, are good candidates for the passivation of defects when deposited onto hybrid lead halide perovskite thin films. Moreover, their semiconductor characteristics can be tailored through the modification of their chemical structure, which allows‐upon light irradiation‐ the interfacial charge transfer between the perovskite film and the truxene molecules. In this work, we analysed the use of the molecules as surface passivation agents and their use in complete functional solar cells. We observed that these molecules reduce the non‐radiative carrier recombination dynamics in the perovskite thin film through the supramolecular complex formation between the Truxene molecule and the Pb2+ defects at the perovskite surface. Interestingly, this supramolecular complexation neither affect the carrier recombination kinetics nor the carriers collection but induced noticeable hysteresis on the photocurrent vs voltage curves of the solar cells under 1 sun illumination.
Chemical Z−E Isomer Switching of Arylazopyrazoles Using Acid
Authors: Gibson Rosina S. L. , Calbo Joaquín , Fuchter Matthew J. 
Published: April 2019

AbstractArylazopyrazoles show significant potential as next‐generation photoswitches, in particular because of the high thermal stability of their Z‐isomers. Herein we investigate the potential to perform Z−E isomer chemical switching of arylazopyrazoles using acid. We show that acid‐accelerated isomerisation allows for robust Z−E switching using mild acids and opens up the possibility to use light‐acid cycles to near quantitatively and reversibly switch the arylazopyrazoles at room temperature. We attribute the chemical switching to azonium formation, facilitated by mesomeric stabilisation by the pyrazole ring. Coupled with their exceptionally long Z‐isomer half‐lives, we believe our study may open up a wider array of functional opportunities for the arylazopyrazoles.
Ruthenium Nanoparticles for Catalytic Water Splitting
Authors: Creus Jordi , De Tovar Jonathan , Romero Nuria , García‐Antón Jordi , Philippot Karine , Bofill Roger , Sala Xavier 
Published: April 2019

AbstractBoth global warming and limited fossil resources make the transition from fossil to solar fuels an urgent matter. In this regard, the splitting of water activated by sunlight is a sustainable and carbon‐free new energy conversion scheme able to produce efficient technological devices. The availability of appropriate catalysts is essential for the proper kinetics of the two key processes involved, namely, the oxygen evolution reaction (OER) and the hydrogen evolution reaction (HER). During the last decade, ruthenium nanoparticle derivatives have emerged as true potential substitutes for the state‐of‐the‐art platinum and iridium oxide species for the HER and OER, respectively. Thus, after a summary of the most common methods for catalyst benchmarking, this review covers the most significant developments of ruthenium‐based nanoparticles used as catalysts for the water‐splitting process. Furthermore, the key factors that govern the catalytic performance of these nanocatalysts are discussed in view of future research directions.
Molecular Catalysts Immobilized on Semiconductor Photosensitizers for Proton Reduction toward Visible‐Light‐Driven Overall Water Splitting
Authors: Morikawa Takeshi , Sato Shunsuke , Sekizawa Keita , Arai Takeo , Suzuki Tomiko M. 
Published: April 2019

AbstractPhotocatalytic or photoelectrochemical hydrogen production by water splitting is one of the key reactions for the development of an energy supply that enables a clean energy system for a future sustainable society. Utilization of solar photon energy for the uphill water splitting reaction is a promising technology, and therefore many systems using semiconductor photocatalysts and semiconductor photoelectrodes for the reaction producing hydrogen and dioxygen in a 2:1 stoichiometric ratio have been reported. In these systems, molecular catalysts are also considered to be feasible; recently, systems based on molecular catalysts conjugated with semiconductor photosensitizers have been used for photoinduced hydrogen generation by proton reduction. Additionally, there are reports that the so‐called Z‐scheme (two‐step photoexcitation) mechanism realizes the solar‐driven uphill reaction by overall water splitting. Although the number of these reports is still small compared to those of all‐inorganic systems, the advantages of molecular cocatalysts and its immobilization on a semiconductor are attractive. This Minireview provides a brief overview of approaches and recent research progress toward molecular catalysts immobilized on semiconductor photocatalysts and photoelectrodes for solar‐driven hydrogen production with the stoichiometric uphill reaction of hydrogen and oxygen generation.
Bushmeat trade consequences predict higher anxiety, restraint, and dominance in chimpanzees
Authors: Ortín Sara , Úbeda Yulán , Garriga Rosa M. , Llorente Miquel 
Published: April 2019

AbstractMore data are needed for a better understanding of the long‐term influence of wider and combined stressful events in chimpanzee personality development. We evaluated the effects of bushmeat trade outcomes on the personality development in 84 African sanctuary chimpanzees. The chimpanzees presented different backgrounds regarding maternal care, social exposure, and abuse. We evaluated personality traits in chimpanzees using the Cattell 16PF personality questionnaire, the first application of this questionnaire in this species. We found that chimpanzees were rated as higher in anxiety after long social deprivation during infancy and juvenility, and if high human exposure was experienced. Mother‐reared chimpanzees were rated as lower in restraint than hand‐reared chimpanzees. Finally, mother‐reared chimpanzees were rated as less dominant than hand‐reared chimpanzees and rated higher when they had experienced severe mistreatment. Results suggest a wide range of possible stressful events could be potentially shaping rescued chimpanzees’ personality and demonstrating the detrimental outcomes and consequences of the bushmeat and pet trade.