bluuebunny/crossref_metadata_2025_split huggingface dataset:
Time-Based prospective memory predicts insight into functional abilities among community-dwelling older adults
Authors: Gereau Mora Michelle , DesRuisseaux Libby A. , Suchy Yana 
Published: December 2024

Abstract

Objective:
Accurate appraisal of one’s own abilities (i.e., insight) is necessary for appropriate compensatory behaviors and sustained independence during aging. Although insight is often purported to be related to executive functioning (EF), nuanced understanding of the cognitive correlates of insight for functional abilities among nondemented older adults is lacking. Because insight shares neuroanatomic underpinnings with time-based prospective memory (PM), the present study examined the contributions of time-based PM, beyond event-based PM and other potential cognitive confounds (i.e., episodic memory, time estimation, and EF), in predicting insight into one’s own performance on instrumental activities of daily living (IADLs) among community-dwelling older adults.

Method:
A group of 88 nondemented, community-dwelling older adults completed performance-based measures of time- and event-based PM, episodic memory, time estimation, and EF, as well as IADL tasks followed by self-appraisals of their own IADL performance as indices of insight.

Results:
Time-based PM was moderately-to-strongly associated with insight, beyond event-based PM, time estimation, and episodic memory [F(1,83) = 11.58, p = .001, ηp2 = .122], as well as beyond EF and demographic covariates [F(1,79) = 10.72, p = .002, ηp2 = .119].Specifically, older adults who performed more poorly on a time-based PM task overestimated the efficiency of their own IADL performance to a greater extent.

Conclusions:
Findings suggest that nondemented older adults with poorer time-based PM may be more prone to inaccurately appraising their functional abilities and that this vulnerability may not be adequately captured by traditional EF measures.
Sustainable development goals and sleep: an integrative review of sleep health disparities and social, economic, and environmental sustainability
Authors: de Andrade Michael Jackson Oliveira 
Published: December 2024

Abstract
Sleep is a topic of scientific and technological relevance, as it is closely related to cognition, emotional well-being, and human health. In addition, health-related geopolitical and social changes have significant implications for sleep research, which in turn is linked to the sustainable development goals (SDGs). An integrative review is conducted to analyze multidisciplinary studies in the field of sleep, considering the socioecological model of sleep health and the SDGs. Sleep was found to play a key role in promoting several SDG: (1) Social and economic inclusion: sleep is linked to poverty eradication, food security, reducing inequalities, and promoting peace and justice; (2) Promoting well-being and quality of life: sleep quality directly affects health, education, gender equality, environmental protection, and combating climate change; (3) Economic and environmental sustainability: Adequate sleep contributes to decent work, innovation, infrastructure, responsible consumption, sustainable communities and collaboration through partnerships. This thematic organization provides an overview of the different aspects of the intersection between sleep health disparities and the SDG.
Estimating the Electric Fields Driving Lightning Dart Leader Development With BIMAP‐3D Observations
Authors: Jensen Daniel P. , Shao Xuan‐Min , Sonnenfeld Richard G. , da Silva Caitano L. 
Published: December 2024

AbstractIn this paper, a numerical dart leader model has been implemented to understand the leader's development and the corresponding electric field changes observed by the 3D broadband mapping and polarization (BIMAP‐3D) system. The model assumes the extending leader channel is equipotential and has a linear charge distribution induced by an ambient electric field. The charge distribution induced by the ambient field can be used to model the electric field change at the ground. We then find the ambient electric field which best fits the field change measurements at the two BIMAP stations. The estimated ambient electric field decreases in the direction of dart leader propagation. Our observations and modeling results are consistent with our earlier hypothesis that dart leader speed is proportional to the electric field at the leader tip. The model also supports our earlier analysis that leader speed variations near branch junctions were due to previous charge deposits near the junctions. The modeled tip electric field is generally lower than the breakdown field unless the pre‐dart‐leader channel has a significant temperature of 3,000 K. This is consistent with the fact that dart leaders typically do not form new branches into the virgin air. Furthermore, the tip field is generally close to the negative streamer stability field at ambient temperatures, explaining the nature of the narrow and well‐defined channel structure. In addition to the charge distribution and the ambient and tip electric field, the development of the channel potential and current distribution are also presented.
β-Hydroxybutyrate suppresses M1 macrophage polarization through β-hydroxybutyrylation of the STAT1 protein
Authors: Bai Ya-Ping , Xing Yu-Jie , Ma Tao , Li Kai , Zhang Teng , Wang De-Guo , Wan Shu-Jun , Zhang Cui-Wei , Sun Yue , Wang Meng-Yan , Wang Guo-Dong , Pei Wen-Jun , Lv Kun , Zhang Yan , Kong Xiang 
Published: December 2024

Abstractβ-Hydroxybutyrate (β-OHB), the primary ketone body, is a bioactive metabolite that acts as both an energy substrate and a signaling molecule. Recent studies found that β-OHB inhibits the production of pro-inflammatory cytokines in macrophages, but its underlying molecular mechanisms have not yet been fully elucidated. Lysine β-hydroxybutyrylation (Kbhb), a post-translational modification mediated by β-OHB, plays a key role in regulating the expression and activity of modified proteins. However, whether macrophages undergo protein Kbhb and whether Kbhb modification regulates macrophage polarization remains largely unknown. In this study, treatment with β-OHB and ketone ester significantly decreased the lipopolysaccharide (LPS)-induced enhancement of the M1 phenotype of mouse bone marrow-derived macrophages (BMDMs), RAW264.7 cells, and peritoneal macrophages (PMs) in vitro and in vivo. Moreover, β-OHB treatment induced global protein Kbhb, which is associated with the regulation of macrophage M1 polarization. Proteome-wide Kbhb analysis in β-OHB-treated BMDMs revealed 3469 Kbhb modification sites within 1549 proteins, among which interleukin-12-responding proteins were significantly upregulated. Our results indicated that β-OHB regulated M1 macrophage polarization by inducing Kbhb modification of the signal transducer and activator of transcription 1 (STAT1) K679 site, which inhibited its LPS-induced phosphorylation and transcription. Altogether, our study demonstrated the presence of a widespread Kbhb landscape in the β-OHB-treated macrophages and provided novel insights into the anti-inflammatory effects of β-OHB.
High-resolution profile of neoantigen-specific TCR activation links moderate stimulation to increased resilience of engineered TCR-T cells
Authors: Füchsl Franziska , Untch Johannes , Kavaka Vladyslav , Zuleger Gabriela , Braun Sarah , Schwanzer Antonia , Jarosch Sebastian , Vogelsang Carolin , de Andrade Krätzig Niklas , Gosmann Dario , Öllinger Rupert , Giansanti Piero , Hiltensperger Michael , Rad Roland , Busch Dirk H. , Beltrán Eduardo , Bräunlein Eva , Krackhardt Angela M. 
Published: December 2024

AbstractNeoantigen-specific T cell receptors (neoTCRs) promise safe, personalized anti-tumor immunotherapy. However, detailed assessment of neoTCR-characteristics affecting therapeutic efficacy is mostly missing. Previously, we identified diverse neoTCRs restricted to different neoantigens in a melanoma patient. In this work, we now combine single-cell TCR-sequencing and RNA-sequencing after neoantigen-specific restimulation of peripheral blood-derived CD8+ T cells of this patient. We detect neoTCRs with specificity for the previously detected neoantigens and perform fine-characterization of neoTCR-transgenic (tg) T cells in vitro and in vivo. We describe a heterogeneous spectrum of TCR-intrinsic activation patterns in response to a shared neoepitope ranging from previously detected more highly frequent neoTCRs with moderate activation to rare ones with initially stronger activation. Experimental restimulation of adoptively transferred neoTCR-tg T cells in a xenogeneic rechallenge tumor model demonstrates superior anti-tumor responses of moderate neoTCR-tg T cells upon repeated tumor contact. These insights have significant implications for the selection of TCRs for therapeutic engineering of TCR-tg T cells.
Frequent transitions in self-assembly across the evolution of a central metabolic enzyme
Authors: Sendker Franziska L. , Schlotthauer Tabea , Mais Christopher-Nils , Lo Yat Kei , Girbig Mathias , Bohn Stefan , Heimerl Thomas , Schindler Daniel , Weinstein Arielle , Metzger Brian P. H. , Thornton Joseph W. , Pillai Arvind , Bange Gert , Schuller Jan M. , Hochberg Georg K. A. 
Published: December 2024

AbstractMany enzymes assemble into homomeric protein complexes comprising multiple copies of one protein. Because structural form is usually assumed to follow function in biochemistry, these assemblies are thought to evolve because they provide some functional advantage. In many cases, however, no specific advantage is known and, in some cases, quaternary structure varies among orthologs. This has led to the proposition that self-assembly may instead vary neutrally within protein families. The extent of such variation has been difficult to ascertain because quaternary structure has until recently been difficult to measure on large scales. Here, we employ mass photometry, phylogenetics, and structural biology to interrogate the evolution of homo-oligomeric assembly across the entire phylogeny of prokaryotic citrate synthases – an enzyme with a highly conserved function. We discover a menagerie of different assembly types that come and go over the course of evolution, including cases of parallel evolution and reversions from complex to simple assemblies. Functional experiments in vitro and in vivo indicate that evolutionary transitions between different assemblies do not strongly influence enzyme catalysis. Our work suggests that enzymes can wander relatively freely through a large space of possible assembly states and demonstrates the power of characterizing structure-function relationships across entire phylogenies.
The first ice-free day in the Arctic Ocean could occur before 2030
Authors: Heuzé Céline , Jahn Alexandra 
Published: December 2024

AbstractProjections of a sea ice-free Arctic have so far focused on monthly-mean ice-free conditions. We here provide the first projections of when we could see the first ice-free day in the Arctic Ocean, using daily output from multiple CMIP6 models. We find that there is a large range of the projected first ice-free day, from 3 years compared to a 2023-equivalent model state to no ice-free day before the end of the simulations in 2100, depending on the model and forcing scenario used. Using a storyline approach, we then focus on the nine simulations where the first ice-free day occurs within 3–6 years, i.e. potentially before 2030, to understand what could cause such an unlikely but high-impact transition to the first ice-free day. We find that these early ice-free days all occur during a rapid ice loss event and are associated with strong winter and spring warming.
Breaking the angular dispersion limit in thin film optics by ultra-strong light-matter coupling
Authors: Mischok Andreas , Siegmund Bernhard , Le Roux Florian , Hillebrandt Sabina , Vandewal Koen , Gather Malte C. 
Published: December 2024

AbstractThin film interference is integral to modern photonics, e.g., allowing for precise design of high performance optical filters, photovoltaics and light-emitting devices. However, interference inevitably leads to a generally undesired change of spectral characteristics with angle. Here, we introduce a strategy to overcome this fundamental limit in optics by utilizing and tuning the exciton-polariton modes arising in ultra-strongly coupled microcavities. We demonstrate optical filters with narrow pass bands that shift by less than their half width (< 15 nm) even at extreme angles. By expanding this strategy to strong coupling with the photonic sidebands of dielectric multilayer stacks, we also obtain filters with high extinction ratios and up to 98% peak transmission. Finally, we apply this approach in flexible filters, organic photodiodes, and polarization-sensitive filtering. These results illustrate how strong coupling provides additional degrees of freedom in thin film optics that will enable exciting new applications in micro-optics, sensing, and biophotonics.
Phosphorylation-mediated conformational change regulates human SLFN11
Authors: Kugler Michael , Metzner Felix J. , Witte Gregor , Hopfner Karl-Peter , Lammens Katja 
Published: December 2024

AbstractHuman Schlafen 11 (SLFN11) is sensitizing cells to DNA damaging agents by irreversibly blocking stalled replication forks, making it a potential predictive biomarker in chemotherapy. Furthermore, SLFN11 acts as a pattern recognition receptor for single-stranded DNA (ssDNA) and functions as an antiviral restriction factor, targeting translation in a codon-usage-dependent manner through its endoribonuclease activity. However, the regulation of the various SLFN11 functions and enzymatic activities remains enigmatic. Here, we present cryo-electron microscopy (cryo-EM) structures of SLFN11 bound to tRNA-Leu and tRNA-Met that give insights into tRNA binding and cleavage, as well as its regulation by phosphorylation at S219 and T230. SLFN11 phosphomimetic mutant S753D adopts a monomeric conformation, shows ATP binding, but loses its ability to bind ssDNA and shows reduced ribonuclease activity. Thus, the phosphorylation site S753 serves as a conformational switch, regulating SLFN11 dimerization, as well as ATP and ssDNA binding, while S219 and T230 regulate tRNA recognition and nuclease activity.
NMR metabolomics as a complementary tool to brix-acid tests for navel orange quality control of long-term cold storage
Authors: Montgomery Keeton H. , Elhabashy Aya , Del Carmen Reynoso Rivas Maria , Brar Gurreet , Krishnan V. V. 
Published: December 2024

AbstractQuality control plays a crucial role in maintaining the reputation of agricultural organizations by ensuring that their products meet the expected standards and preventing any loss during the packaging process. A significant responsibility of quality control is conducting periodic product assessments. However, subjective interpretation during physical inspections of fruits can lead to variability in reporting. To counter this, assessing total soluble solids (Brix) and percent acidity (Acid) can provide a more objective approach. Nevertheless, it is essential to note that many fruit metabolites can impact these parameters. Nuclear magnetic resonance (NMR) spectroscopy, particularly 1H-NMR, has become a popular tool for quality control in recent years due to its precision, sample preservation, and high throughput analysis. This manuscript investigates if the standard Brix/Acid tests are directly related to the levels of metabolites during cold storage. Using citrus as the model system, a metabolomics analysis was conducted to identify patterns in the cold storage metabolite profiles of the juice, albedo, and flavedo tissues. The results show that Brix (or total dissolved solids) correlates well with sucrose, glucose, and fructose levels and moderately with choline levels. Acid (percent acidity) levels displayed a negative correlation with both fructose and choline levels. Interestingly, the formate levels were susceptible to storage time and directly related to Acid measurements. This study suggests metabolomics could be a complementary technique to quality control of fruits in cold storage, especially with cost-effective desktop NMR spectrometers.
Large-scale global retrospective study on the interaction between ancestry and risk of comorbid autoimmune diseases in patients with pemphigus
Authors: Saurabh Rochi , Cani Anikamila , Möller Marius , Busch Hauke 
Published: December 2024

AbstractThe pemphigus family of skin blistering diseases represents a rare yet potentially life-threatening condition characterized by multiple known genetic loci associated with other autoimmune disorders. While several studies have empirically indicated an increased risk of developing additional autoimmune diseases in individuals with pemphigus, the scarcity of data and the rarity of pemphigus have hindered efforts to establish and generalize these associations across diverse populations. In this study, we analyzed a dataset comprising 126 million patients, including 18,000 with pemphigus, to assess the likelihood of developing any of 74 autoimmune diseases following a diagnosis of pemphigus. For a subset of 26 diseases from this list with adequate patient numbers, we conducted further case-control retrospective analyses to quantify the odds and hazard ratios of developing comorbid conditions across various ethnicities. Our findings reveal highly significant and generalizable associations between pemphigus and pemphigoid diseases, discoid lupus erythematosus, lichen planus, and undifferentiated connective tissue disease, among others.
Monitoring May 2024 solar and geomagnetic storm using broadband seismometers
Authors: Díaz J. 
Published: December 2024

Abstract
The abrupt variations in the magnetic field due to the May 2024 geomagnetic storm have been recorded by broadband seismometers distributed around the world for a time interval of more than 55 h. Signals related to magnetic field variations can be identified in seismic data for frequencies below 10 mHz, but are clearer between 1.5 and 5 mHz, the frequency band corresponding to Pc5 magnetic pulsations. The number of seismological stations that detect these signals varies significantly between the various seismic networks analyzed, but in general they do not exceed 50–60%. The origin of these signals appears to be related to the interaction between telluric currents induced by magnetic field disturbances associated with the geomagnetic storm and the detection systems in broadband sensors, which measure the current intensity necessary to keep the mass of the seismometer fixed. Although detailed calibration of each seismometer will be necessary to use the seismic data to model the waveforms and amplitudes of the magnetic pulsations, the analysis of this data provides a significant densification with respect to the magnetic observatory data, as is of interest to analyze the temporal and regional evolution of the magnetics disturbances associated to the May 2024 solar storm.
Cortical tracking of postural sways during standing balance
Authors: Legrand Thomas , Mongold Scott J. , Muller Laure , Naeije Gilles , Ghinst Marc Vander , Bourguignon Mathieu 
Published: December 2024

AbstractMaintaining an upright stance requires the integration of sensory inputs from the visual, vestibular and somatosensory-proprioceptive systems by the central nervous system to develop a corrective postural strategy. However, it is unclear whether and how the cerebral cortex monitors and controls postural sways. Here, we asked whether postural sways are encoded in ongoing cortical oscillations, giving rise to a form of corticokinematic coherence (CKC) in the context of standing balance. Center-of-pressure (CoP) fluctuations and electroencephalographic cortical activity were recorded as young healthy participants performed balance tasks during which sensory information was manipulated, by either removal or alteration. We found that postural sways are represented in ongoing cortical activity during challenging balance conditions, in the form of CKC at 1–6 Hz. Time delays between cortical activity and CoP features indicated that both afferent and efferent pathways contribute to CKC, wherein the brain would monitor the CoP velocity and control its position. Importantly, CKC was behaviorally relevant, as it predicted the increase in instability brought by alteration of sensory information. Our results suggest that human sensorimotor cortical areas take part in the closed-loop control of standing balance in challenging conditions. Importantly, CKC could serve as a neurophysiological marker of cortical involvement in maintaining balance.
Analysis of chemical exchange in iridium N-heterocyclic carbene complexes using heteronuclear parahydrogen-enhanced NMR
Authors: Assaf Charbel D. , Gui Xin , Salnikov Oleg G. , Brahms Arne , Chukanov Nikita V. , Skovpin Ivan V. , Chekmenev Eduard Y. , Herges Rainer , Duckett Simon B. , Koptyug Igor V. , Buckenmaier Kai , Körber Rainer , Plaumann Markus , Auer Alexander A. , Hövener Jan-Bernd , Pravdivtsev Andrey N. 
Published: December 2024

AbstractThe signal amplification by reversible exchange process (SABRE) enhances NMR signals by unlocking hidden polarization in parahydrogen through interactions with to-be-hyperpolarized substrate molecules when both are transiently bound to an Ir-based organometallic catalyst. Recent efforts focus on optimizing polarization transfer from parahydrogen-derived hydride ligands to the substrate in SABRE. However, this requires quantitative information on ligand exchange rates, which common NMR techniques struggle to provide. Here, we introduce an experimental spin order transfer sequence, with readout occurring at 15N nuclei directly interacting with the catalyst. Enhanced 15N NMR signals overcome sensitivity challenges, encoding substrate dissociation rates. This methodology enables robust data fitting to ligand exchange models, yielding substrate dissociation rate constants with higher precision than classical 1D and 2D 1H NMR approaches. This refinement improves the accuracy of key activation enthalpy ΔH‡ and entropy ΔS‡ estimates. Furthermore, the higher chemical shift dispersion provided by enhanced 15N NMR reveals the kinetics of substrate dissociation for acetonitrile and metronidazole, previously inaccessible via 1H NMR due to small chemical shift differences between free and Ir-bound substrates. The presented approach can be successfully applied not only to isotopically enriched substrates but also to compounds with natural abundance of the to-be-hyperpolarized heteronuclei.