bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel bioassay for thyroid-blocking immunoglobulins
Authors: George Augustine , Lotz Johannes , Luffy Maximilian , Ganz Anna-Lena , Wolf Jan , Kahaly George J. 
Published: October 2024

BackgroundThyroid-blocking immunoglobulins (TBI) are present in 10%–15% of patients with autoimmune thyroid disease (AITD). TBI affect thyroid function. The analytical performance of a novel TBI bioassay was evaluated.MethodsSera from AITD patients were tested with a cell-based TBI reporter bioassay (Thyretain®) with the expression of a luciferase transgene as readout and a new “Turbo™” TBI bioassay with a readout based on a cyclic AMP-activated luciferase. All samples were also run on two TSH-R binding immunoassays. A Passing–Bablok regression, a Bland–Altman plot, and user/lot comparisons were performed. In addition, dose–response curves for Turbo and Thyretain were fitted using serial dilutions, and half-maximal and 80% inhibitory concentrations (IC50/IC80) were compared.ResultsOf 1,011 unselected AITD patients, 131 patients (212 samples) were TBI positive. Of the 212 samples, 149 (70.3%), 47 (22%), and 16 (7.5%) were hypothyroid, euthyroid, and hyperthyroid, respectively. The three thyrotropin receptor antibody (TSH-R-Ab) assays were negative in 90 controls devoid of autoimmune thyroid disorders. In contrast, the Turbo cyclic adenosine 3′,5′-monophosphate (cAMP) TBI, Thyretain TBI, and the binding assays detected TBI in 212 (100%), 168 (79%), and 138/180 (65%) samples, respectively (p&lt; 0.001). Turbo highly correlated with thyroid function (p&lt; 0.001). The percentage inhibition in both Turbo and Thyretain correlated with TSH-R-Ab binding assay positivity (both p&lt; 0.001). The two bioassays correlated (r = 0.8, p&lt; 0.001), and the Bland–Altman plot displayed no significant bias (0.24). Values scatter with slight systemic deviation between TBI mean values of 10%–50% inhibition, with higher Turbo than Thyretain results. Intra-assay validation demonstrated adequate precision with a very low coefficient of variation (average CV 5.4%) and lower CV with samples with a high inhibitory effect (CVAverage= 1.7% for a sample with 95% inhibition Thyretain). CV did not differ between users (p = 0.35) and lots (p = 0.121). The IC50/IC80 values were 1.55 ng/mL/3.48 ng/mL for Turbo and 6.76 ng/mL/18.46 ng/mL for Thyretain, respectively, demonstrating the markedly higher sensitivity of Turbo.ConclusionsThe novel, easy-to-perform, rapid, and reliable Turbo TSH-R blocking bioassay detected significantly more TBI than the established immunoassays, emphasizing its higher analytical performance and clinical utility in the management of patients with AITD.
Skeletal phenotypes in postmenopausal women affected by primary hyperparathyroidism
Authors: Corbetta Sabrina , Gianotti Laura , Castellano Elena , Carrara Silvia , Raineri Francesca , Munari Elisabetta , Guabello Gregorio , Cairoli Elisa , Chiodini Iacopo , Giovanelli Luca , Pierotti Laura , Dinoi Elisa , Della Valentina Simone , Cetani Filomena 
Published: October 2024

PurposeThe current primary hyperparathyroidism (PHPT) presents as a mild disease. We explored skeletal phenotypes in postmenopausal women affected by PHPT, focusing on fracture prevalence.MethodsPHPT women were retrospectively evaluated at four Italian centers for osteoporosis management (two centers in Milan, n = 244; Cuneo, n = 128; Pisa, n = 131). Data collected from clinical records were analyzed by hierarchical clusterization.ResultsConsidering the whole PHPT series [n = 503, aged 67.0 (61.0–74.0) years], 90% had low bone mineral density (BMD) and approximately 30% reported at least one fracture. Vertebral fractures were associated with older age and lower hypophosphatemia, while women with appendicular fractures were younger with less severe hypophosphatemia. Fractures were predicted by lumbar T-score. By using a clustering approach, we identified four different skeletal phenotypes (cluster, C): C1 (n = 53) and C2 (n = 172) included women with lumbar and femur neck osteopenia, with low prevalence of fractures (11.3%). Osteoporotic PHPT women were grouped into C3 (n = 142) and C4 (n = 136); all women in C4 experienced fractures, were older, and were more frequently affected with cardiovascular diseases. In contrast, women included in C3 never experienced fractures and had a lower body mass index (BMI), though they were characterized by severe reduction in BMD at both lumbar and femur sites. Ionized and total calcium, phosphate, 25hydroxyvitamin D levels, kidney function, and stone prevalence (range, 26.4%–29.0%) were similar among clusters C1, C2, and C4, while unfractured women in C3 showed slightly higher ionized hypercalcemia, lower hypophosphatemia, and higher hypercalciuria with a trend to more frequently develop kidney stones (38.7%) than women in the remaining clusters.ConclusionsSkeletal involvement in women with PHPT presented heterogeneous phenotypes with different prevalence of fractures. Fractures were not related to PHPT severity, suggesting that other factors besides PHPT, such as age, BMI, and lumbar and femur BMD, should be considered in the evaluation of bone involvement in postmenopausal women with PHPT.
Has risk management plan system influenced the speed of package insert revisions in Japan?
Authors: Kameyama Natsuko , Hosaka Aoi , Maeda Hideki 
Published: October 2024

IntroductionThe system of Risk Management Plan in Japan (J-RMP) is a relatively new system, implemented in 2013; thus, its effect on safety measures is still unclear. One of the purposes of J-RMP is to enhance the postmarketing safety measures to be ensured by publishing J-RMP and sharing information on risk management among healthcare professionals. We hypothesized that this might enable information about postmarketing adverse events to be accumulated rapidly, potentially accelerating the identification of adverse reactions (ARs). Herein, we focused on the speed of adding clinically significant ARs (CSARs) to package inserts (PIs) as an indicator of the rapidity of AR identification, investigated the impact of the J-RMP system on PI revisions.MethodsWe investigated the “Notice of Revision of Precautions” on the website of Pharmaceuticals and Medical Devices Agency (PMDA), targeting PI revisions with the addition of CSARs from April 2003 to March 2023, which corresponds to 10 years before and after J-RMP implementation in April 2013. We created an original database from public information of PMDA and investigated the speed of adding CSARs to PIs.ResultsComparing the time lapse from drug approvals to PI revisions after J-RMP implementation (149 cases) to that before implementation (318 cases), the median value was 32 months for both. Regarding the time lapse when the additional CSARs were listed and unlisted as safety concerns at the time of approvals, it was 35 months vs. 32 months (14 cases vs. 126 cases, p = 0.7820), with no statistically significant difference. Conversely, there were significant differences within each AR and each drug therapeutic category.Discussion and conclusionsThis study revealed that the rapidity of risk identification as ARs was not affected by J-RMP, and it may be affected by the characteristics of each AR and each drug therapeutic category. It is expected that other J-RMP benefits, such as risk prevention before the occurrence, will be utilized to further develop strategies for the effective utilization of the J-RMP for safety measures in Japan.
Large language models in patient education: a scoping review of applications in medicine
Authors: Aydin Serhat , Karabacak Mert , Vlachos Victoria , Margetis Konstantinos 
Published: October 2024

IntroductionLarge Language Models (LLMs) are sophisticated algorithms that analyze and generate vast amounts of textual data, mimicking human communication. Notable LLMs include GPT-4o by Open AI, Claude 3.5 Sonnet by Anthropic, and Gemini by Google. This scoping review aims to synthesize the current applications and potential uses of LLMs in patient education and engagement.Materials and methodsFollowing the PRISMA-ScR checklist and methodologies by Arksey, O’Malley, and Levac, we conducted a scoping review. We searched PubMed in June 2024, using keywords and MeSH terms related to LLMs and patient education. Two authors conducted the initial screening, and discrepancies were resolved by consensus. We employed thematic analysis to address our primary research question.ResultsThe review identified 201 studies, predominantly from the United States (58.2%). Six themes emerged: generating patient education materials, interpreting medical information, providing lifestyle recommendations, supporting customized medication use, offering perioperative care instructions, and optimizing doctor-patient interaction. LLMs were found to provide accurate responses to patient queries, enhance existing educational materials, and translate medical information into patient-friendly language. However, challenges such as readability, accuracy, and potential biases were noted.DiscussionLLMs demonstrate significant potential in patient education and engagement by creating accessible educational materials, interpreting complex medical information, and enhancing communication between patients and healthcare providers. Nonetheless, issues related to the accuracy and readability of LLM-generated content, as well as ethical concerns, require further research and development. Future studies should focus on improving LLMs and ensuring content reliability while addressing ethical considerations.
An updated phylogeography and population dynamics of porcine circovirus 2 genotypes: are they reaching an equilibrium?
Authors: Franzo Giovanni , Tucciarone Claudia Maria , Legnardi Matteo , Drigo Michele , Segalés Joaquim 
Published: October 2024

IntroductionPorcine circovirus 2 (PCV2) emerged more than three decades ago as one of the most impactful pathogens in the swine industry. Despite being a DNA virus, one of the hallmarks of PCV2 is its high evolutionary rate, which has led to the emergence of different genotypes, each exhibiting varying degrees of evolutionary success. Current knowledge suggests the occurrence of three main waves of genotype dominance, alternating over time (i.e., PCV2a, PCV2b, and PCV2d), alongside less prevalent genotypes. However, although PCV2d is currently the most common genotype nowadays, the others continue being circulating in the pig population.MethodsThe present study reconsidered the epidemiological and evolutionary patterns of PCV2 genotypes using phylodynamic analyses, benefiting from an almost 10-fold increase in ORF2 sequence availability compared to previous studies. Additionally, a phylogeographic analysis was performed to investigate viral dispersal patterns and frequency, and the selective pressures acting on the capsid protein were estimated and compared among genotypes.ResultsWhile successive emergence of major genotypes was confirmed, this study extends previous findings by revealing subsequent prevalence fluctuations of PCV2a and PCV2b after their initial decline. This evolutionary process may represent an example of balancing selection, specifically negative frequency-dependent selection, where a genotype fitness diminishes as it becomes more common, and vice versa. Variations in genotype- or clade-specific immunity—affected by the local prevalence of viral groups—combined with the periodic introduction of strains that have independently evolved in different regions, may have led to fluctuations in the population dynamics of major genotypes over time. These fluctuations were associated with ongoing evolution and variations in the capsid amino acid profile.DiscussionThese findings have profound implications for future control strategies. Although PCV2d remains the most prevalent and widespread genotype, other genotypes should not be neglected. Control strategies should thus target the entire PCV2 population, with a focus on fostering broader and more cross-protective immunity.
Social contagion of pain and fear results in opposite social behaviors in rodents: meta- analysis of experimental studies
Authors: Du Rui , Yu Yang , Wang Xiao-Liang , Lu Guofang , Chen Jun 
Published: October 2024

IntroductionThe study aimed to explore the key factors influencing emotional valence in rodents, focusing on the critical elements that distinguish the contagion processes of fear and pain.MethodsThrough a systematic review and meta-analysis, we examined behavioral outcomes of rodents exposed to painful or fearful catastrophes to see whether they are prosocial or antisocial through three-chamber test and dyadic social interaction paradigm.ResultsFear contagion, particularly when witnessed, leads to social avoidance behavior, unaffected by sex difference but more pronounced with age. In contrast, pain contagion promotes social approach and caring/helping behaviors.DiscussionThe present study demonstrates that the emotional valence induced by pain contagion is quite different from fear contagion and this difference may result in different motivations and social behaviors, namely, social contagion of pain is likely to be more associated with prosocial behaviors, however, social contagion of fear is likely to be more associated with antisocial behaviors.Systematic Review RegistrationPROSPERO (CRD42024566326).
Primary hepatoid adenocarcinoma of the lung in patient with silicosis: a case report and literature review
Authors: Huang Lipeng , Chen Chaoyang , Sun Qingyu , Yu Zhichen , Wang Xiaoyan , Wang Xinle , Yang Shuoqi , Jin Luming , Bu Liang 
Published: October 2024

IntroductionHepatoid adenocarcinoma of the lung (HAL) is a special type of adenocarcinoma originating from the lung with adenoid- and hepatocyte-like differentiation. HAL is rare in clinical practice. Here, we present the case of a patient with HAL.Case presentationA 59-year-old man was admitted to the hospital 4 days because of lung mas observed. Chest computed tomography (CT) revealed a lobulated mass shadow in the right lower lobe, approximately 3.5 × 3.3 cm in size. CT-guided percutaneous biopsy of the right lower lung was performed. The pathological results indicated a moderately to poorly differentiated adenocarcinoma. The patient underwent thoracoscopic right middle and lower lobectomy and systematic lymph node dissection. The postoperative pathology was primary HAL, with the staging of T2bN2M0 (stage III A). Recurrence-free survival and overall survival were 6 and 19 months, respectively Preoperatively, the level of alpha-fetoprotein was negative; however, after recurrence, it increased to 87.8.ConclusionPulmonary hepatoid adenocarcinoma is a rare subtype of malignant lung tumor, combined silicosis is more rare. Early surgical intervention can benefit patients in the early stages of the disease, whereas chemotherapy remains the main systemic treatment modality for postoperative and advanced stages. With the increasing popularity of genetic testing, it is important to focus on improving genetic examination.
The effects of maternal voice on pain during placement of peripherally inserted central catheter in neonates
Authors: Flours Audrey , Mons Fabienne , Bedu Antoine , Lauvray Thomas , Blanquart Anne-Laure , Woillard Jean-Baptiste , Mowendabeka Audrey , Guigonis Vincent , Ponthier Laure 
Published: October 2024

BackgroundPeripherally inserted central catheter (PICC) are a necessary procedure for preterm newborns care. Despite the use of analgesic treatments, its insertion can be painful. Our objective was to study the effect of maternal voice on pain during PICC insertion.MethodWe conducted a pre post study for 2 years. Pain was compared between the two groups (with/without maternal presence) using a neonatal pain scale (FANS). Infection rate, procedure time, number of failures, mothers’ anxiety and caregivers’anxiety were compared between the two groups.ResultsNinety neonates were eligible. Finally, 63 neonates were included. Thirty-four placements were realized without maternal voice (first period) and 29 with maternal voice (second period). Mean FANS during PICC placement was lower in the maternal voice group than in the control group (1.15 ± 1.27 vs. 1.41 ± 1.49, p = 0.033). The FANS was also lower in the maternal voice group during the time of the first cutaneous effraction (p = 0.032). There was no significant difference between the two groups concerning the other outcomes.ConclusionMaternal voice added to conventional care decreased acute pain during PICC insertion without increasing infection rate, number of failures or procedure time.
The psychological mechanism of basic psychological need frustration affecting job burnout: a qualitative study from China
Authors: Shi Hairong 
Published: October 2024

IntroductionJob burnout is a common issue in most professions, and it can have adverse effects on employees, their families, clients, and organizations. It is essential to address and resolve job burnout syndrome. More research is needed to understand the underlying psychological mechanisms involved in job burnout. This study introduces the concept of primary psychological need frustration to explore its impact on the psychological processes involved in job burnout.MethodsThis study adopted a qualitative research methodology based on purposive sampling and convenience sampling principles. Eight grassroots senior counselors with over 13 years of teaching experience at a Chinese university were selected as the study cases. The data were gathered through semi-structured interviews and analyzed thoroughly via cluster analysis, which involved examining the text data word by word, sentence by sentence, line by line, and fragment by fragment. NVivo 11 software was used to register and code the text data.ResultsThe study revealed that the subjects experienced high levels of frustration with their basic psychological needs. This frustration was evident in the coexistence of negative job characteristics and a lack of autonomy, a hostile professional environment and a lack of competence, and the negative behavior of others and relationship frustration. The study also revealed that the four types of primary psychological need frustration were strongly linked to job burnout: A lack of control motivation or motivation, the pursuit of external goals, negative behavior patterns, and the causal orientation of a controlled style. These factors positively predicted various dimensions of job burnout and positively affected the frustration of basic psychological needs.ConclusionThis study effectively explains the psychological process behind why individuals experience severe job burnout in a controlled organizational environment due to frustration with basic psychological needs. This study also highlights the internal causal relationship between primary psychological needs, frustration, and job burnout. This insight can help employees and organizations prevent and detect early job burnout syndrome and enhance employees’ occupational well-being and organizational vitality.
Classification of recovery states in U15, U17, and U19 sub-elite football players: a machine learning approach
Authors: Teixeira José E. , Encarnação Samuel , Branquinho Luís , Ferraz Ricardo , Portella Daniel L. , Monteiro Diogo , Morgans Ryland , Barbosa Tiago M. , Monteiro António M. , Forte Pedro 
Published: October 2024

IntroductionA promising approach to optimizing recovery in youth football has been the use of machine learning (ML) models to predict recovery states and prevent mental fatigue. This research investigates the application of ML models in classifying male young football players aged under (U)15, U17, and U19 according to their recovery state. Weekly training load data were systematically monitored across three age groups throughout the initial month of the 2019–2020 competitive season, covering 18 training sessions and 120 observation instances. Outfield players were tracked using portable 18-Hz global positioning system (GPS) devices, while heart rate (HR) was measured using 1 Hz telemetry HR bands. The rating of perceived exertion (RPE 6–20) and total quality recovery (TQR 6–20) scores were employed to evaluate perceived exertion, internal training load, and recovery state, respectively. Data preprocessing involved handling missing values, normalization, and feature selection using correlation coefficients and a random forest (RF) classifier. Five ML algorithms [K-nearest neighbors (KNN), extreme gradient boosting (XGBoost), support vector machine (SVM), RF, and decision tree (DT)] were assessed for classification performance. The K-fold method was employed to cross-validate the ML outputs.ResultsA high accuracy for this ML classification model (73–100%) was verified. The feature selection highlighted critical variables, and we implemented the ML algorithms considering a panel of 9 variables (U15, U19, body mass, accelerations, decelerations, training weeks, sprint distance, and RPE). These features were included according to their percentage of importance (3–18%). The results were cross-validated with good accuracy across 5-fold (79%).ConclusionThe five ML models, in combination with weekly data, demonstrated the efficacy of wearable device-collected features as an efficient combination in predicting football players’ recovery states.
The relationship between sense of coherence and self-efficacy with well-being and mental health—the situation of students at a typical German university during the COVID-19 pandemic and 1 year after the lifting of social restrictions
Authors: Kösler Lisa-Marie , Bauer Stephanie , Möltner Andreas , Holm-Hadulla Rainer M. 
Published: October 2024

IntroductionThe present study aims to evaluate the role of sense of coherence and self-efficacy in relation to mental health and well-being, with and without social restrictions due to the COVID-19 pandemic. Additionally, this study seeks to investigate the differences in sense of coherence, self-efficacy, mental health and well-being depending on the manner in which the pandemic is being handled.MethodsA total of 27,162 students at Heidelberg University were surveyed via email at two measurement points, once with and once without social restrictions. The survey assessed sense of coherence, self-efficacy, mental health and well-being. To this end, the questionnaires Sense of Coherence Scale, Patient Health Questionnaire, WHO-Well-being-Index and the General Self-Efficacy Scale were employed. A total of 2,398 individuals participated in the initial measurement, while 701 individuals participated in the subsequent measurement.ResultsThe lifting of social restrictions has been associated with a notable improvement in well-being and mental health, particularly in the context of depressive syndromes. Further analysis demonstrated a positive correlation between the sense of coherence and self-efficacy at both measurement points, as well as between these two constructs and mental health and well-being. Furthermore, the sense of coherence and self-efficacy were found to account for a notable proportion of the observed variability in mental health and well-being values. Self-efficacy exhibited a significantly higher mean value at the initial measurement time point compared to the subsequent time point. In contrast, no significant difference was observed in the sense of coherence between the two measurement points.DiscussionThe findings presented here illustrate the significance of social interaction, sense of coherence and self-efficacy for mental health and well-being.