bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of therapeutic plasma exchange on angiotensin II type‐1 receptor antibodies on two kidney transplant recipients
Authors: Yamada Chisa , Huang Yihung , Norman Silas , Naik Abhijit , Moussa Omar , Samaniego Milagros , Cooling Laura 
Published: October 2018

AbstractBackgroundAngiotensin II type‐1 receptor antibody (AT1RAb) has been reported to cause antibody mediated rejection (AMR) in kidney transplant recipients possibly by contraction of renal arteries. We here report 2 kidney transplant recipients with elevated AT1RAbs and negative HLA donor specific antibodies (DSA) and anti‐major histocompatibility complex class I chain‐related gene A (MICA) Abs who received therapeutic plasma exchange (TPE) treatment followed by IVIG.Case 1Thirty‐eight‐year‐old patient received second kidney transplant for end stage renal disease (ESRD) with chronic rejection. Three years post‐transplant, she developed AMR with AT1RAb level >40 U/mL. She received 5 TPE and AT1RAb decreased by 20%, and biopsy showed improvement of AMR. She received another 3 TPE and AT1RAb decreased by 60%. Her creatinine (Cr) was stabilized at around 1.4 mg/dL.Case 2Twenty‐four‐year‐old patient received kidney transplant for ESRD with unclear etiology. Two weeks post‐transplant, her Cr rose with AT1RAb level at 18 U/mL and biopsy showed possible AMR. She received 6 TPE treatments and AT1RAb decreased by 55% and biopsy showed improvement of AMR. She received weekly TPE for subsequently rising AT1RAb but TPE was discontinued because of unsuccessful decrease of AT1RAb. Her Cr was stabilized at around 1.7 mL/dL.ConclusionWe reported 2 patients who received TPE treatments to decrease AT1RAbs. A course of TPE treatment successfully decreased AT1RAb. Histological improvement was observed quickly and Cr was also stabilized following the TPE treatment. Further study is necessary to determine the optimal use of TPE in renal transplant recipients with AT1RAbs.
Comparison of echocardiography and 64‐slice spiral computed tomography in the diagnosis of congenital heart disease in children
Authors: Mei Mei , Nie Jing , Yang Zhan Shuang , Sun Hua Wei , Wang He , Kang Xiao Ming 
Published: October 2018

AbstractThe diagnosis of congenital heart disease in children has been an issue in the medical community. Timely diagnosis and treatment can provide a greater guarantee for children's healthy growth. In recent years, there have been more and more studies on the diagnosis of congenital heart disease in children. This paper compares the advantages and disadvantages of echocardiography and 64‐slice spiral computed tomography (CT) in the diagnosis of congenital heart disease in children. In clinical trials, we also tested 64 patients with spiral computed tomography (SCT) and transthoracic echocardiography (TTE) detection of patients and then confirmed the accuracy of the diagnosis by the surgical methods. The two methods of detection, the rate of missed diagnosis, and the rate of misdiagnosis were counted. Through the test results and pathological diagnosis results, the diagnostic accuracy of the two methods were all above 90%, each with its own advantages and disadvantages. The sensitivity of echocardiographic in detecting intracardiac structure abnormalities was relatively high, but when the diagnosis of extracardiac structural abnormalities less than 64‐slice spiral CT method, misdiagnosis of TTE was mainly due to extracardiac vascular malformations. Therefore, it is recommended to combine the two methods to improve the diagnosis of congenital heart disease in children.
Retracted: Receptor for advanced glycation end products reveals a mechanism regulating thyroid hormone secretion through the SIRT1/Nrf2 pathway
Authors: Chen Xiao‐Jun , Gong Xiao‐Hua , Jie Jin‐Ping , Yu Wei‐Hui , Chen Xiong , Du Xuan , Zhou Qi , Wu Wen‐Jun 
Published: October 2018

AbstractAdvanced glycation end products (AGEs) play a causative role in the complications involved with diabetes mellitus (DM). Nowadays, DM with hypothyroidism (DM‐hypothyroidism) is indicative of an ascended tendency in the combined morbidity. In this study, we examine the role of the receptor (RAGE) played for AGEs in thyroid hormone (TH) secretion via the silent information regulator 1 (SIRT1)/nuclear factor erythroid‐derived factor 2‐related factor 2 (Nrf2) pathway. Blood samples were collected from patients with type 2 DM (T2DM)‐hypothyroidism and from patients with T2DM, followed by detection of serum AGEs level. The underlying regulatory mechanisms of RAGE were analyzed in association with the treatment of high glucose, siRNA against RAGE, AGE, SIRT1, or Nrf2 vector in normal immortalized thyroid Nthy‐ori 3‐1 cells. Serum of patients with T2DM‐hypothyroidism indicated promoted levels of AGEs vs those with just T2DM. Both AGEs and high glucose triggered cellular damage, increased oxidative stress, as well as displayed a decreased survival rate along with TH secretion in the Nthy‐ori 3‐1 cells. Moreover, AGEs and high glucose also led to RAGE upregulation, both SIRT1 and NRF2 downregulation, and the decreased expression of TH secretion–related proteins in Nthy‐ori 3‐1 cells. Notably, these alternations induced by the AGEs can be reserved by silencing RAGE or upregulating either SIRT1 or Nrf2, indicating a mechanism of regulating TH secretion through the SIRT1/Nrf2 pathway. Collectively, our data proposed that AGEs and high glucose exerted a potent effect on cellular damage and TH deficiency in Nthy‐ori 3‐1 cells through the RAGE upregulation as well as SIRT1/Nrf2 pathway inactivation. This mechanism may underlie the occurrence of DM‐hypothyroidism.
Delphinidin induces antiproliferation and apoptosis of endometrial cells by regulating cytosolic calcium levels and mitochondrial membrane potential depolarization
Authors: Park Sunwoo , Lim Whasun , Song Gwonhwa 
Published: October 2018

AbstractEndometriosis is a benign gynecological disease of women of reproductive ages, wherein endometrial cells grow ectopically, decreasing their quality of life due to chronic pelvic pain and severe dysmenorrhea. Although surgery and hormone therapies are gold standards for treating endometriosis, side effects are common and the recurrence rate is nearly 50%. Recent studies are exploring phytochemicals as pharmacological adjuvants for treating endometriosis. Delphinidin is an anthocyanin with anti‐inflammatory, antioxidative, and anticancerous properties. In this study, delphinidin showed antiproliferative and apoptotic effects on human endometrial cells. Additionally, treatment with delphinidin decreased the mitochondrial membrane potential and increased cytosolic calcium levels in VK2/E6E7 and End1/E6E7 cells. Delphinidin decreased the phosphorylation of proliferative signaling molecules, including ERK1/2, AKT, P70S6K, and S6, while increasing the phosphorylation of P38 MAPK and P90RSK. These results imply that delphinidin is a novel therapeutic agent for treating and managing endometriosis, and has fewer side effects.
Retracted: Downregulation of microRNA‐1469 promotes the development of breast cancer via targeting HOXA1 and activating PTEN/PI3K/AKT and Wnt/β‐catenin pathways
Authors: Zhang Yonghui , Fang Jing , Zhao Hongmeng , Yu Yue , Cao Xuchen , Zhang Bin 
Published: October 2018

AbstractBreast cancer (BC) is a common malignancy which is the most frequently diagnosed cancer in women all over the worldwide. This study aimed to investigate the roles of miR‐1469 in the development of BC, as well as its regulatory mechanism. The expression levels of miR‐1469 in BC tissues, serum, and cell lines were determined. Effects of overexpression of miR‐1469 on MCF7 cell viability, colony‐forming ability, apoptosis, migration, and invasion were then investigated. Furthermore, the potential target of miR‐1469 in MCF7 cells was explored. Besides, the association between miR‐1469, PTEN/PI3K/AKT, and Wnt/β‐catenin pathways was elucidated. Notably, confirmatory experiments by downregulation of miR‐1469 in SK‐BR‐3 cells were further performed. The miR‐1469 expression was significantly downregulated in BC tissues, serum, and cell lines. The overexpression of miR‐1469 significantly inhibited the proliferation, arrested cell‐cycle at G2/M phase, increased apoptosis, suppressed migration, and invasion of MCF‐7 cells. In addition, HOXA1 was verified as a direct target of miR‐1469, and the effects of overexpression of miR‐1469 on the malignant behaviors of MCF7 cells were significantly counteracted by overexpression of HOXA1 concurrently. Furthermore, the overexpression of miR‐1469 suppressed the activation of PTEN/PI3K/AKT and Wnt/β‐catenin pathways, which was reversed overexpression of HOXA1 concurrently. Besides, confirmatory experiments showed that the inhibition of miR‐1469 promoted the malignant behaviors of SK‐BR‐3 cells, which was inversed after miR‐1469 inhibition and HOXA1 knockdown at the same time. Our findings reveal that downregulation of miR‐1469 may promote the development of BC by targeting HOXA1 and activating PTEN/PI3K/AKT and Wnt/β‐catenin pathways. MiR‐1469 may serve as a promising target for BC therapy.
LncRNA and mRNA signatures associated with neoadjuvant chemoradiotherapy downstaging effects in rectal cancer
Authors: Li Ning , Yu Jing , Luo Aiping , Tang Yuan , Liu Wenyang , Wang Shulian , Liu Yueping , Song Yongwen , Fang Hui , Chen Bo , Qi Shunan , Lu Ningning , Yu Zihao , Li Yexiong , Liu Zhihua , Jin Jing 
Published: October 2018

AbstractRadiotherapy plays a crucial role in combined treatment modality in local advanced rectal cancer (LARC). While neoadjuvant chemoradiotherapy responses were variable in LARC patients, so, it is important to identify genes that closely associated with short‐term and long‐term responses to radiotherapy. In this study, we profiled long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression values of LARC patients with different neoadjuvant chemoradiotherapy downstaging depth score based on Agilent Arraystar Human LncRNA V3.0 Array(Agilent, CA). LncRNAs and mRNAs with aberrant expression values between the two groups of LARC patients were identified and lncRNA‐miRNA‐mRNA regulation network was also obtained through the combination of miRcode and miRTarBase database. Gene interaction network and module analysis of differential expression mRNAs contained in the lncRNA‐miRNA‐mRNA network identified five hub genes, including KRAS, PDPK1, PPP2R5C, PPP2R1B, and YES1, that should be closely associated with LARC’s response to chemoradiotherapy. Besides, Kaplan‐Meier analysis based on the Cyber Research Center (CRC) data set from The Cancer Genome Atlas indicated that aberrant expression of the five hub genes is significantly associated with CRC overall survival. In conclusion, we obtained several biomarkers that should be associated with neoadjuvant chemoradiotherapy response in LARC, which should be helpful for individual treatment and prognosis improvement.
Signal transducer and activator of transcription 3 inhibition enhances vemurafenib sensitivity in colon cancers harboring the BRAFV600E mutation
Authors: Wang Kai , Li Yanrong , Song Na , Che Xiaofang , Hou Kezuo , Xu Ling , Bai Ming , Wang Qiwei , Wang Yuanhe , Zhou Yang , Cao Meihui , Liu Yunpeng , Zhang Jingdong 
Published: October 2018

AbstractThe BRAFV600E inhibitor vemurafenib is widely used to treat melanomas harboring the activated BRAFV600E mutation; however, vemurafenib showed poor efficacy in colon cancer, which impeded its clinical application for colon cancer patients with this mutation. The specific mechanism of vemurafenib resistance is not clear in colon cancer. In this study, we demonstrated that signal transducer and activator of transcription 3 (STAT3) activation influenced vemurafenib sensitivity in BRAFV600E mutant colon cancer cells. When vemurafenib was applied to two colon cancer cell lines with the BRAFV600E mutation, STAT3 was continuously activated after 6 hours. Furthermore, BCL‐2 was upregulated in RKO colon cancer cells, while STAT3 remained unchanged in HT‐29 colon cancer cells. This suggested that STAT3 signaling might be involved in vemurafenib sensitivity. Combining the STAT3 inhibitor STATTIC with vemurafenib further inhibited cell proliferation and promoted apoptosis by downregulating STAT3 and BCL‐2 expression in RKO cells. Further studies showed that interleukin 6 (IL‐6) secretion increased after RKO cells were treated with vemurafenib. STAT3 activation was induced by adding IL‐6 to the supernatant, and IL‐6 increased STAT3 and BCL‐2 expression and antagonized vemurafenib sensitivity in HT‐29 cells. Together, these results suggest that STAT3 activation maybe related to vemurafenib sensitivity in colon cancer cells, and that combining STAT3 inhibitors with vemurafenib may be a promising treatment for BRAFV600E mutant colon cancers.
Immune‐endocrine responses and physical performance of master athletes during the sports season
Authors: Minuzzi Luciele Guerra , Rama Luis , Chupel Matheus Uba , Rosado Fátima , Kuga Gabriel Keine , Gaspar Rafael Calais , Muñoz Vitor Rosetto , Pauli José Rodrigo , Paiva Artur , Teixeira Ana Maria 
Published: October 2018

AbstractThe purpose of this study was to investigate the impact of a training season (approximately 7 months) on physiological and salivary immune‐endocrine markers in master athletes. Nine male master athletes were evaluated at the beginning of the season (M1) and a week after the main official competition at the end of the sports season (M2). The controlled variables included Maximal oxygen consumption, anthropometric, physiological, and salivary immune‐endocrine markers. Master athletes presented a reduced percentage of fat mass and increased lean body mass at the end of the season. VO2max values were similar at M1 and M2, while the maximal heart rate and lactate were lower at M2. No differences were observed in Immunoglobulin A and cortisol levels between moments, whereas testosterone levels and the testosterone/cortisol ratio were significantly lower at the end of the season. The results suggest that maintaining regular training throughout life has positive effects on body composition and improves physiological fitness. However, care should be taken to avoid fatigue as indicated by lower testosterone levels at the end of the season.
The oncolytic virus H101 combined with GNAQ siRNA‐mediated knockdown reduces uveal melanoma cell viability
Authors: Li Yongyun , He Jie , Qiu Chun , Shang Qingfeng , Qian Guanxiang , Fan Xianqun , Ge Shengfang , Jia Renbing 
Published: October 2018

AbstractBackgroundUveal melanoma (UM) is a severe human malignancy with a high mortality rate, as well as high metastasis and recurrence potential. The active mutation of G protein subunit alpha q (GNAQ) or G protein subunit alpha 11 (GNA11) is a major trigger for UM. Oncolytic adenovirus H101 (H101) is the first oncolytic virus approved for clinical applications in cancer therapy by the China Food and Drug Administration. We investigated whether combining H101 with the downregulation of GNAQ expression would act synergistically in UM therapy.MethodsThree UM cell lines OMM2.3 and 92.1, harboring GNAQ mutation, and OCM1, harboring B‐Raf proto‐oncogene mutation, were chosen for our research. The cellular toxicity of adenoviral infection and the cell growth rate were measured with a Cell Counting Kit‐8. Western blot analysis was used to detect GNAQ, p‐MEK1/2, YAP, and p‐YAP expression. The apoptosis and cell‐cycle distribution of cells were evaluated with annexin‐V and propidium iodide staining.ResultsOur results revealed that OMM2.3 and 92.1 cells were more sensitive to H101 infection than OCM1 cells. GNAQ expression was markedly reduced by small interfering RNA, siGNAQ. Combined treatment of siGNAQ and H101 inhibited the proliferation and activated the apoptosis of OMM2.3 and 92.1 cells by blocking the phosphorylation of MEK1/2 and increasing the phosphorylation of YAP.ConclusionsIn summary, a therapy combining H101 and siGNAQ is feasible, with potential utility as a novel targeted molecular therapy for UM, especially those carrying a GNAQ mutation.
Promoting osteogenic differentiation of human‐induced pluripotent stem cells by releasing Wnt/β‐catenin signaling activator from the nanofibers
Authors: Hosseini Fatemeh Sadat , Soleimanifar Fatemeh , Khojasteh Arash , Ardeshirylajimi Abdolreza 
Published: October 2018

AbstractImplants that can enhance the stem cells differentiation in the absence of the chemical osteogenic growth factors will attract the great interest of orthopedic scientists. Inorganic polyphosphate (poly‐P), as a ubiquitous biological polymer, is one of the factors that can be an alternative for osteogenic growth factors via activating Wnt/β‐catenin signaling. In this study, poly‐P was incorporated at the blend of polycaprolactone (PCL)/poly (l‐lactic acid) (PLLA) electrospun nanofibers and then osteogenic differentiation potential of human‐induced pluripotent stem cells (iPSCs) was investigated by the important bone markers. 3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5 diphenyl tetrazolium bromide (MTT) and scanning electron microscopy results confirmed the biocompatibility of the fabricated nanofibers, while higher proliferation rate of iPSCs was detected in PCL‐PLLA(poly‐P) group compared with the PCL‐PLLA and tissue culture plate groups. Alkaline phosphatase activity, calcium content, and gene expression results demonstrated that osteogenic differentiation of iPSCs was increased when cultured on PCL‐PLLA(poly‐P) in comparison with other groups. According to the results, PCL‐PLLA(poly‐P) could be considered as a promising candidate for use as bone implants.
Analysis of differentially expressed circular RNAs for the identification of a coexpression RNA network and signature in colorectal cancer
Authors: Zhang Zheying , Song Na , Wang Yongxia , Zhong Jiateng , Gu Tengteng , Yang Liping , Shen Xianfeng , Li Yuelin , Yang Xin , Liu Xiangyu , Yang Rui , Wang Haijun 
Published: October 2018

AbstractCircular RNAs (circRNAs) play an important regulatory role in tumorigenesis. The aim of the present study was to analyze the circRNA expression network and elucidate its potential implications in colorectal cancer (CRC). The circRNA expression profile was analyzed in CRC tissues by RNA sequencing, and the functions of differentially expressed genes were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The circRNA network was predicted with bioinformatics. On the basis of the results, we identified 23 differentially expressed circRNAs in CRC; GO and KEGG analyses demonstrated that the changes in circRNAs were mainly associated with regulation of biological and metabolic processes through binding to other molecules. In addition, based on the predicted coexpression network, we identified a hub circRNA, hsa_circ_0009022. Subsequently, the results of sequencing were confirmed by reverse transcription‐quantitative polymerase chain reaction, and hsa_circ_0000826 was found to be downregulated in CRC. Taken together, these findings indicate a set of differentially expressed circRNAs that may serve as a candidate diagnostic biomarker and a promising therapeutic target in CRC.
Plasmapheresis for hypertriglyceridemia: The association between blood viscosity and triglyceride clearance rate
Authors: Wu Hung‐Chieh , Lee Lin‐Chien , Wang Wei‐Jie 
Published: October 2018

ObjectivesSeveral factors in double filtration plasmapheresis (DFPP) were associated with triglyceride (TG) clearance rate. This study examines whether baseline whole blood viscosity was a predictor for efficient TG removal.MethodsAdult subjects who receiving DFPP for hyperlipidemia in Taoyuan General Hospital from January 2015 to March 2018 were classified into efficient and inefficient TG removal according to TG removal rate ≥50% vs <50%. TG removal rate was defined as following formula: (pre‐apheresis

TG‐ post‐apheresis TG)/pre‐apheresis TG. Whole blood viscosity (WBV) was estimated by following equation: WBV = 0.12 × hematocrit +0.17 × (total protein −2.07). Univariate linear regression was used to assess the association between TG removal rate and WBV. Odds ratios (ORs) and 95% confidence interval (95%CI) for associations between variables and efficient TG removal were evaluated by logistic regression model to including univariate and multivariate adjustment.ResultsFrom a total of 66 subjects receiving DFPP, 37 subjects reached efficient TG removal. The difference in pre‐apheresis TG levels, hematocrit, and WBV between efficient vs. inefficient TG removal groups was 4.1 vs 6.7 mmol/L; 43.1% vs 39.5%; and 6.0cP vs 5.cP (Ps <0.05). After multivariate adjustment, WBC was a significant predictor for efficient TG removal (ORs and 95% CI were 3.192 (1.300‐7.838), P < 0.05). The correlation between WBV and extraction of TG was significant (r = −0.255, P = 0.039).ConclusionHyperviscosity reduced the efficiency of TG removal in those receiving DFPP.
Global expression profiling of metabolic pathway‐related lncRNAs in human gastric cancer and the identification of RP11‐555H23.1 as a new diagnostic biomarker
Authors: Mo Xiaoyan , Wu Yuanyuan , Chen Li , Zhai Ming , Gao Zhengdong , Hu Kainan , Guo Junming 
Published: October 2018

BackgroundLong noncoding RNAs (lncRNAs) have roles in regulating metabolism; however, the global expression profile of metabolic pathway‐associated lncRNAs in gastric cancer is unknown. The purpose of our study was to examine metabolic pathway‐related lncRNAs in gastric cancer and their possible diagnostic values.MethodsDifferential expression patterns of metabolic pathway‐related lncRNAs between gastric cancer and paired nontumor tissues were detected using metabolic pathway‐associated lncRNA microarrays. The expression of RP11‐555H23.1, one representative metabolic pathway‐associated lncRNA, was validated using quantitative real‐time reverse transcription‐polymerase chain reaction (qRT‐PCR). The associations between RP11‐55H23.1 expression and the clinicopathological features of gastric cancer patients were analyzed. A receiver operating characteristic (ROC) curve was further established.ResultsA total of 114 differentially expressed metabolic pathway‐associated lncRNAs (fold change >2, P < 0.05) between cancer and nontumor tissues were found (GEO No. GSE96856). Among them, TUG1, RP11‐555H23.1, RP1‐257I20.13, UGP2, GCSHP3, and XLOC_000889 lncRNAs were downregulated more than sixfold in gastric cancer tissues. In contrast, RP11‐605F14.2, TBC1D3P5, BC130595, LINC00475, RP11‐19P22.6, BC080653, XLOC_004923, AFAP1‐AS1, EPB49, and RP11‐296I10.3 lncRNAs were upregulated more than sixfold in gastric cancer tissues. We further demonstrated that RP11‐555H23.1 expression was significantly correlated with TNM stage (P = 0.038). The area under the ROC curve (AUC) was 0.65, and the specificity and sensitivity were 62% and 81%, respectively.ConclusionsMetabolic pathway‐associated lncRNAs play an important role in the occurrence of gastric cancer, and metabolic pathway‐associated lncRNAs, such as RP11‐555H23.1, may represent novel biomarkers of gastric cancer.