bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early-Life Predictors of Systolic Blood Pressure Trajectories From Infancy to Adolescence: Findings From Project Viva
Authors: Aris Izzuddin M , Rifas-Shiman Sheryl L , Li Ling-Jun , Belfort Mandy B , Hivert Marie-France , Oken Emily 
Published: August 2019

AbstractChildhood blood pressure (BP) is a strong predictor of later risk of cardiovascular disease. However, few studies have assessed dynamic BP trajectories throughout the early-life period. We investigated the relationship between early-life factors and systolic BP (SBP) from infancy to adolescence using linear spline mixed-effects models among 1,370 children from Project Viva, a Boston, Massachusetts-area cohort recruited in 1999–2002. After adjusting for confounders and child height, we observed higher SBP in children exposed to gestational diabetes mellitus (vs. normoglycemia; age 3 years: β = 3.16 mm Hg (95% confidence interval (CI): 0.28, 6.04); age 6 years: β = 1.83 mm Hg (95% CI: 0.06, 3.60)), hypertensive disorders of pregnancy (vs. normal maternal BP; age 6 years: β = 1.39 mm Hg (95% CI: 0.10, 2.67); age 9 years: β = 1.84 mm Hg (95% CI: 0.34, 3.34); age 12 years: β = 1.70 mm Hg (95% CI: 0.48, 2.92)), higher neonatal SBP (per 10-mm Hg increase; age 3 years: β = 1.26 mm Hg (95% CI: 0.42, 2.09); age 6 years: β = 1.00 mm Hg (95% CI: 0.49, 1.51); age 9 years: β = 0.75 mm Hg (95% CI: 0.17, 1.33)), and formula milk in the first 6 months of life (vs. breast milk only; age 12 years: β = 2.10 mm Hg (95% CI: 0.46, 3.74); age 15 years: β = 3.52 mm Hg (95% CI: 1.40, 5.64); age 18 years: β = 4.94 mm Hg (95% CI: 1.88, 7.99)). Our findings provide evidence of programming of offspring SBP trajectories by gestational diabetes, hypertensive disorders of pregnancy, and formula milk intake and of neonatal BP being a potentially useful marker of childhood BP. These factors could be relevant in identifying children who are at risk of developing elevated BP.
Controlled substance diversion in health systems: A failure modes and effects analysis for prevention
Authors: Nolan Karen , Zullo Andrew R , Bosco Elliott , Marchese Christine , Berard-Collins Christine 
Published: August 2019

AbstractPurposeThe purpose of this study was to demonstrate the utility of failure modes and effects analysis (FMEA) for systematically identifying potential sources of controlled substance diversion and developing solutions in an academic health system.MethodsThe FMEA was conducted by an 18-member cross-functional team from the department of pharmacy. The team developed scoring criteria specifically for controlled substance diversion, outlined the controlled substance processes from procurement to administration or disposal, and identified ways in which each step of the medication supply process might fail (failure modes) and result in diversion of controlled substances. Failure modes with a vulnerability score of 48 or 64 were considered highest risk and were immediately intervened on by the FMEA team.ResultsThe FMEA outlined a total of 10 major steps and 30 substeps in the controlled substance supply process. From this, 103 potential failure modes were identified, with 24 modes (23%) receiving a vulnerability score of 48 or 64. Development of specific reports addressed 15 failure modes, while 9 involved pharmacy workflow alterations. Notable reports included controlled substance activity under temporary patients and discrepancy trends by user, medication, and patient care area. Notable workflow alterations included expanded use of cameras in high-risk areas and additional verification checks.ConclusionFMEA allowed for systematic identification of controlled substance diversion opportunities, prioritization by level of vulnerability, and the development of targeted strategies to reduce risk of diversion.
Adipose-Derived Stem Cells for Regenerative Wound Healing Applications: Understanding the Clinical and Regulatory Environment
Authors: Luck Joshua , Weil Benjamin D , Lowdell Mark , Mosahebi Afshin 
Published: August 2019

Abstract
There is growing interest in the regenerative potential of adipose-derived stem cells (ADSCs) for wound healing applications. ADSCs have been shown to promote revascularization, activate local stem cell niches, reduce oxidative stress, and modulate immune responses. Combined with the fact that they can be harvested in large numbers with minimal donor site morbidity, ADSC products represent promising regenerative cell therapies. This article provides a detailed description of the defining characteristics and therapeutic potential of ADSCs, with a focus on understanding how ADSCs promote tissue regeneration and repair. It summarizes the current regulatory environment governing the use of ADSC products across Europe and the United States and examines how various adipose-derived products conform to the current UK legislative framework. Advice is given to clinicians and researchers on how novel ADSC therapeutics may be developed in accordance with regulatory guidelines.
baerhunter: an R package for the discovery and analysis of expressed non-coding regions in bacterial RNA-seq data
Authors: Ozuna A , Liberto D , Joyce R M , Arnvig K B , Nobeli I 
Published: August 2019

Abstract

Summary
Standard bioinformatics pipelines for the analysis of bacterial transcriptomic data commonly ignore non-coding but functional elements e.g. small RNAs, long antisense RNAs or untranslated regions (UTRs) of mRNA transcripts. The root of this problem is the use of incomplete genome annotation files. Here, we present baerhunter, a coverage-based method implemented in R, that automates the discovery of expressed non-coding RNAs and UTRs from RNA-seq reads mapped to a reference genome. The core algorithm is part of a pipeline that facilitates downstream analysis of both coding and non-coding features. The method is simple, easy to extend and customize and, in limited tests with simulated and real data, compares favourably against the currently most popular alternative.

Availability and implementation
The baerhunter R package is available from: https://github.com/irilenia/baerhunter

Supplementary information
Supplementary data are available at Bioinformatics online.
On the frequency of northern and mountain genetic variants of widespread species: essential biodiversity information in a warmer world
Authors: Hedenäs Lars 
Published: August 2019

AbstractBiodiversity studies and conservation management often neglect fundamental genetic diversity, even if biodiversity loss due to reduction in genetic diversity in declining, relatively common species is probably greater than the loss resulting from disappearing rare species. Climate models suggest that global warming will especially affect high latitudes due to a higher than average temperature increase towards the poles. In widespread Northern Hemisphere species, climate warming will therefore negatively affect especially northern and mountain genetic variants (NMGVs) in the same way that it will affect species with similar distributions. To explore whether NMGVs are frequent, they were searched for in ten widespread moss species based on a wide geographical sampling in Sweden and (two-) three (-four) molecular markers per species. NeighborNet split networks and jackknife analyses showed that eight of the studied species include one or more northern or, in most cases, mountain intraspecific genetic lineages or groups of related haplotypes. Analysis of molecular variance also showed that in the two species lacking such lineages or groups the genetic composition differs between different parts of Sweden or between lowlands and mountains of the north. Haplotype or nucleotide diversity is often higher in the north or mountains than in the south or lowlands. The high incidence of NMGVs, differences in genetic composition between the study regions and the frequent high diversity in the north or mountains show that conservation of the total biodiversity must also include the genetic diversity, not just the more obvious species-level taxa with restricted northern or mountain distributions.
Intermediate outcomes of right-to-left inverted living-donor lobar lung transplantation
Authors: Chen-Yoshikawa Toyofumi F , Tanaka Satona , Yamada Yoshito , Yutaka Yojiro , Nakajima Daisuke , Ohsumi Akihiro , Hamaji Masatsugu , Menju Toshi , Date Hiroshi 
Published: August 2019

AbstractOBJECTIVESOwing to the severe donor shortage in Japan, living-donor lobar lung transplantation (LDLLT) remains a valuable option. As only lobes are implanted in LDLLT, grafts may be too small, especially for adult recipients. To overcome this obstacle, we developed right-to-left inverted LDLLT. In this procedure, the right lower lobe, which is 25% bigger than the left lower lobe, is used as the left-side graft instead of the left lower lobe. This study aimed to investigate the characteristics and intermediate outcomes of right-to-left inverted LDLLT.METHODSSince the first right-to-left inverted LDLLT performed in 2014, 48 LDLLTs have been performed in our institution, of which 15 were right-to-left inverted LDLLTs. We reviewed their characteristics and intermediate outcomes.RESULTSThe reasons for choosing an inverted procedure instead of the standard LDLLT were small-for-size graft in 11 cases and anatomical variation of donor vessels in 4 cases. The first patient underwent left single LDLLT using a right lower lobe graft, and the following 14 patients underwent bilateral LDLLT using 2 right lower lobe grafts. A native upper lobe-sparing procedure was additionally applied in 2 patients. No complications occurred in the bronchial and vascular anastomoses. No operative mortality occurred, and all the patients were discharged home after LDLLT. The 3-year survival was 92.3%, with a median follow-up time of 40 months. The donor postoperative course was uneventful, and all the donors returned to their regular routine postoperatively.CONCLUSIONRight-to-left inverted LDLLT is a safe and useful option with encouraging intermediate outcome.
Anaerobic degradation of 2-chloro-4-nitroaniline by Geobacter sp. KT7 and Thauera aromatica KT9
Authors: Duc Ha Danh 
Published: August 2019

ABSTRACT
2-chloro-4-nitroaniline is a nitroaromatic compound widely used in industrial and agricultural sectors, causing serious environmental problems. This compound and some of its analogs were utilized by two Fe3+-reducing microbial strains Geobacter sp. KT7 and Thauera aromatica KT9 isolated from contaminated sediment as sole carbon and nitrogen sources under anaerobic conditions. The anaerobic degradation of 2-chloro-4-nitroaniline by the mixed species was increased approximately by 45% compared to that of individual strains. The two isolates’ crossfeeding, nutrient sharing and cooperation in the mixed culture accounted for the increase in degradation rates. The determination of degradation pathways showed that Geobacter sp. KT7 transformed the nitro group in 2-chloro-4-nitroaniline to the amino group following by the dechlorination process, while T. aromatica KT9 dechlorinated the compound before removing the nitro group and further transformed it to aniline. This study provided an intricate network of 2-chloro-4-nitroaniline degradation in the bacterial mixture and revealed two parallel routes for the substrate catabolism.
Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expression system
Authors: Takagi Shinobu , Tsutsumi Noriko , Terui Yuji , Kong XiangYu , Yurimoto Hiroya , Sakai Yasuyoshi 
Published: August 2019

ABSTRACT
The construction of a methanol-free expression system of Komagataella phaffii (Pichia pastoris) was attempted by engineering a strong methanol-inducible DAS1 promoter using Citrobacter braakii phytase production as a model case. Constitutive expression of KpTRM1, formerly PRM1—a positive transcription regulator for methanol-utilization (MUT) genes of K. phaffii,was demonstrated to produce phytase without addition of methanol, especially when a DAS1 promoter was used but not an AOX1 promoter. Another positive regulator, Mxr1p, did not have the same effect on the DAS1 promoter, while it was more effective than KpTrmp1 on the AOX1 promoter. Removing a potential upstream repression sequence (URS) and multiplying UAS1DAS1 in the DAS1 promoter significantly enhanced the yield of C. braakii phytase with methanol-feeding, which surpassed the native AOX1 promoter by 80%. However, multiplying UAS1DAS1 did not affect the yield of methanol-free expression by constitutive KpTrm1p. Another important region to enhance the effect of KpTrm1p under a methanol-free condition was identified in the DAS1 promoter, and was termed ESPDAS1. Nevertheless, methanol-free phytase production using an engineered DAS1 promoter outperformed phytase production with the GAP promoter by 25%. Difference in regulation by known transcription factors on the AOX1 promoter and the DAS1 promoter was also illustrated.
Missing in Analysis: Women in Foreign Policy–Making
Authors: Smith Karen E 
Published: August 2019

AbstractForeign policy analysis (FPA) opens the “black box” of the state and provides explanations of how and why foreign policy decisions are made, which puts individuals and groups (from committees to ministries) at the center of analysis. Yet the sex of the decision-maker and the gendered nature of the decision-making process have generally been left out of the picture. FPA has not addressed questions regarding the influence of women in foreign policy decision-making processes or the effects of gender norms on decision-making; indeed, FPA appears to be almost entirely gender-free. This article argues that “gendering” FPA is long overdue and that incorporating gender into FPA frameworks can provide a richer and more nuanced picture of foreign policy–making.
Evidence of Extensive Intraspecific Noncoding Reshuffling in a 169-kb Mitochondrial Genome of a Basidiomycetous Fungus
Authors: Lee Hsin-Han , Ke Huei-Mien , Lin Chan-Yi Ivy , Lee Tracy J , Chung Chia-Lin , Tsai Isheng J 
Published: August 2019

AbstractComparative genomics of fungal mitochondrial genomes (mitogenomes) have revealed a remarkable pattern of rearrangement between and within major phyla owing to horizontal gene transfer and recombination. The role of recombination was exemplified at a finer evolutionary time scale in basidiomycetes group of fungi as they display a diversity of mitochondrial DNA inheritance patterns. Here, we assembled mitogenomes of six species from the Hymenochaetales order of basidiomycetes and examined 59 mitogenomes from 2 genetic lineages of Phellinus noxius. Gene order is largely collinear, while intergene regions are major determinants of mitogenome size variation. Substantial sequence divergence was found in shared introns consistent with high horizontal gene transfer frequency observed in yeasts, but we also identified a rare case where an intron was retained in five species since speciation. In contrast to the hyperdiversity observed in nuclear genomes of Phellinus noxius, mitogenomes’ intraspecific polymorphisms at protein-coding sequences are extremely low. Phylogeny network based on introns revealed turnover as well as exchange of introns between two lineages. Strikingly, some strains harbor a mosaic origin of introns from both lineages. Analysis of intergenic sequence indicated substantial differences between and within lineages, and an expansion may be ongoing as a result of exchange between distal intergenes. These findings suggest that the evolution in mitochondrial DNAs is usually lineage specific but chimeric mitotypes are frequently observed, thus capturing the possible evolutionary processes shaping mitogenomes in a basidiomycete. The large mitogenome sizes reported in various basidiomycetes appear to be a result of interspecific reshuffling of intergenes.
Over 1000 genetic loci influencing blood pressure with multiple systems and tissues implicated
Authors: Cabrera Claudia P , Ng Fu Liang , Nicholls Hannah L , Gupta Ajay , Barnes Michael R , Munroe Patricia B , Caulfield Mark J 
Published: August 2019

Abstract
High blood pressure (BP) remains the major heritable and modifiable risk factor for cardiovascular disease. Persistent high BP, or hypertension, is a complex trait with both genetic and environmental interactions. Despite swift advances in genomics, translating new discoveries to further our understanding of the underlying molecular mechanisms remains a challenge. More than 500 loci implicated in the regulation of BP have been revealed by genome-wide association studies (GWAS) in 2018 alone, taking the total number of BP genetic loci to over 1000. Even with the large number of loci now associated to BP, the genetic variance explained by all loci together remains low (~5.7%). These genetic associations have elucidated mechanisms and pathways regulating BP, highlighting potential new therapeutic and drug repurposing targets. A large proportion of the BP loci were discovered and reported simultaneously by multiple research groups, creating a knowledge gap, where the reported loci to date have not been investigated in a harmonious way. Here, we review the BP-associated genetic variants reported across GWAS studies and investigate their potential impact on the biological systems using in silico enrichment analyses for pathways, tissues, gene ontology and genetic pleiotropy.
Optimizing Egg Recovery From Wild Northern Corn Rootworm Beetles (Coleoptera: Chrysomelidae)
Authors: Pereira Adriano E , Ludwick Dalton C , Barry Julie , Meinke Lance J , Moellenbeck Daniel J , Ellersieck Mark R , Reinders Jordan D , Geisert Ryan W , Hyte Keiran , Ernwall Amanda , Paddock Kyle J , Hibbard Bruce E 
Published: August 2019

AbstractThe northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae), is one of the most important insect pests in the U.S. Corn Belt. Efforts to obtain eggs from wild northern corn rootworm populations using techniques developed for other rootworm species have been unsuccessful due to lack of oviposition. In 2016, we evaluated four oviposition media in choice tests within each of three female densities in 30.5 × 30.5 × 30.5 cm BugDorm cages. The number of eggs laid per female was significantly affected by female density and the interaction of female density × oviposition media, but oviposition was relatively poor in all oviposition media (1.2 eggs per female when averaging the three female densities and all oviposition media). Single females were also evaluated in nonchoice assays in 6 cm × 6 cm × 8 cm clear plastic boxes and averaged up to 108 eggs per female depending on the oviposition media. In 2017, the cumulative number of eggs laid per female in boxes with one female was not significantly different from the number of eggs laid per female in boxes with 3 females. In 2018, the cumulative number of eggs laid per female was not significantly different between female densities of 1, 3, 5, or 10 females per box. Total egg production per box therefore increased as female density increased. More than 27,000 wild northern corn rootworm eggs were collected from just 190 females when collected relatively early in the field season. We now have an efficient and robust system for obtaining eggs from wild northern corn rootworm females.
A Codling Moth (Lepidoptera: Tortricidae) Kairomonal Lure Is Marginally Effective at Decreasing Fruit Damage in Apple Trees Outside of Orchard Settings
Authors: Jaffe Benjamin D , Landolt Peter J 
Published: August 2019

Abstract
Codling moth, Cydia pomonella (L.), is a significant pest of pome fruits and walnuts worldwide. Recently, a three-chemical kairomonal lure, comprised of pear ester, acetic acid, and n-butyl sulfide, was successfully used as an attractant in a mass-trapping scheme to reduce fruit damage in commercial apple orchards. In this study, we tested whether this same attractant could be used outside of an orchard setting to decrease fruit damage in isolated, unmanaged apple (Malus spp.) (Rosales: Rosaceae) trees. Traps containing the lures were placed in trees before the first codling moth flight and maintained throughout the summer. We found that while the traps statistically reduced the percent of apples damaged near the trap, the effect was smaller than expected and limited to areas near the trap. It is currently unclear, but site-specific effects (e.g., host type, apple density, codling moth source) may be important factors in the efficacy of management tools in these systems. While kairomone-based trapping could be a practical and feasible management tool in individual trees outside of orchards, more work needs to be done to understand the limitations of this method.
Resistance of Fruits From a Mandarin Cultivar to Feeding by Fork-Tailed Bush Katydids
Authors: Cass Bodil N , Grafton-Cardwell Elizabeth E , Rosenheim Jay A 
Published: August 2019

Abstract
Plants use a variety of mechanisms to defend against herbivore damage, each with different consequences for agricultural production. Crops relying on tolerance strategies may need different pest management approaches versus those relying on resistance strategies. Previous work suggested that densities of fork-tailed bush katydids (Scudderia furcata Brunner von Wattenwyl [Orthoptera: Tettigoniidae]) that generated substantial scarring on cultivars of sweet oranges (Citrus sinensis, (L.) Osbeck [Sapindales: Rutaceae]) produced only low levels of scarring on cultivars of Citrus reticulata Blanco mandarins. We used field experiments in representative cultivars of these species to test non-mutually exclusive hypotheses regarding the mechanisms underlying this observation: 1) katydids are averse to feeding on mandarin fruits, 2) damaged mandarin fruits preferentially abscise, 3) damaged mandarin fruit tissue recovers during development, and 4) katydid scars on mandarins have a different morphology that may result in misclassification. We found strong support for the first hypothesis, demonstrating that katydids reject opportunities to feed on C. reticulata fruit. Instead of chewing deep holes in the fruit, as was commonly observed for C. sinensis, the katydids only scratched the surface of the C. reticulata fruits. The hypotheses of preferential abscission of damaged fruits and of recovery of damaged tissue were not supported. The low incidence of damage to the mandarins prevented a comprehensive assessment of the scar morphology; however, at harvest, the superficial cuts in C. reticulata were not easily distinguishable from background damage. This indicates that in contrast to C. sinensis, C. reticulata has substantial natural resistance to fork-tailed bush katydids making them a non-pest in this crop.
Creativity at the Edge of Chaos: Theopoetics in a Blazing World
Authors: Walton Heather 
Published: August 2019

Abstract
Theopoetics is a diverse movement in which many contrasting understanding of poetics, and the sacred, are articulated. Although there is no essence or core to the theopoetic project, theopoetic practitioners appear willing to sustain a common space for conversation and their work displays a common celebration of creative ways for reimagining the divine. However, creativity itself is rarely interrogated within theopoetics. Nor is the ambiguity of creative making addressed. This article explores this significant but neglected topic in an attempt to generate further theopoetic reflection on the ambivalence of creativity in relation to both human and divine ‘making’. Starting with conversations between Richard Kearney and Catherine Keller, discussions are extended through an engagement with Siri Hustvedt’s works of art criticism and her novel The Blazing World. Through Husdtvedt’s representations of ‘the woman artist’ we are encouraged to move beyond hygienic understandings of creativity and acknowledge the ethical and theological ambiguity of this key concept within theopoetics.