bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of tyrosinase‐catalyzed crosslinking on the structure and allergenicity of turbot parvalbumin mediated by caffeic acid
Authors: Tian Shenglan , Ma Jiaju , Ahmed Ishfaq , Lv Liangtao , Li Zhenxing , Lin Hong 
Published: January 2019

AbstractBACKGROUNDEnzymatic treatment of allergenic protein can alter their functional properties under a mild reaction condition due to specificity of enzymes. Phenolic compounds act as mediators and enhance the crosslinking reactions. The study aimed to assess the changes in the structure and immunoglobulin G (IgG) binding capacity of turbot parvalbumin (PV) upon crosslinking with tyrosinase (Tyr) in the absence and presence of caffeic acid.RESULTSSodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) analysis revealed the appearance of higher molecular weight bands (24, 36 kDa) in the crosslinked PV. The secondary structure of crosslinked PV became loosened and disordered. The results of intrinsic fluorescence and ultraviolet absorption spectral analyses, as well as surface hydrophobicity and free amino group analyses also revealed structural changes. As observed by western blot analysis, the intensity of the PV bands reduced upon Tyr treatment, indicating reduced binding of specific IgG to PV. Moreover, the indirect ELISA (enzyme‐linked immunosorbent assay) analysis confirmed that the IgG binding ability of crosslinked PV was reduced 34.94%.CONCLUSIONEnzymatic treatment mitigated the allergenicity of fish PV, which was closely related to the alterations in the conformational structure. This treatment showed potential for developing hypoallergenic fish products under mild reaction conditions. © 2019 Society of Chemical Industry
Combination of thermal pretreatment and alcohol‐assisted aqueous processing for rapeseed oil extraction
Authors: Tian Lingyu , Ren Yanqin , Yang Ruijin , Zhao Qiyan , Zhang Wenbin 
Published: January 2019

AbstractBACKGROUNDThe alcohol‐assisted aqueous extraction processing (AAEP) of oil has many advantages such as no need for demulsification and relative low cost compared with enzymatic aqueous extraction processing (EAEP). Three kinds of thermal pretreatments including dry‐heating, wet‐heating and soak‐heating followed by the AAEP of rapeseed oil were investigated.RESULTSBoth soak‐heating and wet‐heating had a higher contribution rate to oil yield than dry‐heating due to the enhancement of heat transfer rate owing to the high moisture content in the rapeseed cells. However, oil from soak‐heated rapeseeds showed a much lower level on peroxide value (0.41 mmol kg−1) than that of wet‐heated rapeseeds (5.23 mmol kg−1). In addition, transmission electron microscopy images illustrated that promoting effects of soak‐heating and wet‐heating on oil release were attributed to the coalescence of oil bodies. A relative low concentration of alcohol solution as an extraction medium, the highest oil recovery of 92.77% was achieved when ground rapeseeds (mean particle size: 21.23 µm) were treated with 45% (v/v) alcohol for 2 h at 70 °C and pH 9.0. Both the acid value and the peroxide value are lower than the commercial oil produced by extrusion and hexane extraction. Furthermore, the oil produced from AAEP also had higher content of tocopherols and lower content of trans‐fatty acids than the commercial oil.CONCLUSIONAAEP of oil from soak‐heated rapeseeds is a promising alternative to conventional oil extraction methods. © 2019 Society of Chemical Industry
The effect of using different types and rates of onion‐water extract in meatball production on the formation of heterocyclic aromatic amines
Authors: Nuray Meryem , Oz Fatih 
Published: January 2019

AbstractBACKGROUNDThe effect of using different types (yellow, white and purple) and rates (0.25%, 0.50% and 0.75%) of onion‐water extract in meatball production on the various quality criteria of meatballs and the formation of heterocyclic aromatic amines (HCAs) was investigated.RESULTSIt was determined that cooking process caused a reduction in the water content of meatballs as well as an increase in pH values. MeIQx, 7,8‐DiMeIQx, 4,8‐DiMeIQx, PhIP, AαC and MeAαC could not be detected in any of the analyzed meatballs, while IQx, IQ and MeIQ were determined in some of the samples.CONCLUSIONThe use of 0.25% and 0.50% white onion‐water extract in meatball preparation inhibited the formation of IQx and IQ that were identified in the control group, but their quantities could not be determined. However, the use of water extract of varying types of onions in meatball preparation increased the total HCA content due to the increase of the MeIQ formation. In addition, it was determined that as the rate of onion‐water extract for varying types of onions increased, the total HCA amount also increased. The total HCA amount of the meatballs varied between 0.05 and 0.1 ng g−1. © 2019 Society of Chemical Industry
The visual aesthetics measurement on interface design education
Authors: Wu Chun Mao , Li Pei 
Published: January 2019

AbstractVisual aesthetics measurement has been studied in the information processing. Indeed, it was mainly conducted by designers and experts previously, which is related to evaluate final works of visual aesthetics. By contrast, relations of inspirations and outcomings are largely ignored in the field of interface design. The result was measured by students, users, and experts, so as to understand the different effects of the interface design inspirations. Two studies were to investigate the influences of inspirations, such as the image‐verbal–oriented (task 1) and the interface‐image–oriented (task 2), and measure the outcomings by users, experts, and participants works. Thirty participants, two experts, and two users, rated the interface design works. The code frequency and correlation analysis were adopted. The findings demonstrate that usability and aesthetics are scored highly in task 2 that is supported by the rating from users and experts. The originality and creativity are rated superior in task 1 than task 2 that is rated by experts. This study reveals that image‐verbal–oriented inspiration matches to the creativity and originality in interface design, and the interface‐image–oriented inspiration was related to the usability and aesthetics, which contribute to the interface design processing and inspiration adoption.
Degradation and renaturalization of soils affected by tourist activity in the Bieszczady Mountains (South East Poland)
Authors: Drewnik Marek , Musielok Łukasz , Prędki Ryszard , Stolarczyk Mateusz , Szymański Wojciech 
Published: January 2019

AbstractThe main aims of this study were to determine the degree of soil degradation caused by hikers along tourist trails in broad‐leaved forests and subalpine meadows in the Bieszczady Mts. (SE Poland) using the entire thickness of the soil profile and to determine both the direction and rate of renaturalization of degraded soils experiencing renaturalization over different periods of time. Seven representative plots (out of 25) were selected for detailed study. On the research plots, three different but representative research sites arranged perpendicularly to the trail were selected: (a) type R representing soil located in the center of the fenced area and still experiencing renaturalization, (b) type T representing soil on the tourist trail currently being used, and (c) type N representing natural soil showing lack of signs of trampling. The studied soils affected by tourists have become degraded substantially over time to a varying degree. This was manifested by a truncation of the soil profile and by soil compaction. The maximum depth of erosion was found to be ~0.5 m. Stones forming a ‘post‐erosional pavement’ play a crucial role in limiting deeper soil erosion. Both the loosening of renaturalized soils resulting from freeze–thaw cycles and from needle‐ice formation occur relatively quick, although the formation of a topsoil A horizon is a slow process. A humid climate and soils exhibiting high‐soil organic matter content and high‐cation exchange capacity due to their loamy texture facilitate relatively fast restoration of the vegetation cover, which protects the soil from erosion.
Engaging patients, clinicians, and the community in a Clinical Data Research Network: Lessons learned from the CAPriCORN CDRN
Authors: Tambor Ellen , Shalowitz Madeleine , Harrington Joseph M. , Hull Kevin , Watson Natalie , Sital Shelly , Al Naber Jennifer , Miller Doriane 
Published: January 2019

AbstractEngaging patients, clinicians, and community members in the development of a research network creates opportunities and challenges beyond engagement in discrete learning activities. This paper describes our experiences establishing and maintaining a stakeholder engagement infrastructure for the Chicago Area Patient‐Centered Outcomes Research Network (CAPriCORN) and highlights important lessons learned over the first 4 years. During this time, the CAPriCORN Patient and Community Advisory Committee (PCAC) appointed patient, clinician, and community representatives to governance and advisory groups throughout the network, developed a process and criteria for patient‐ and clinician‐centered review of research proposals, and evolved from a large, diverse group to a smaller yet still diverse, more actively engaged group with connections to the broader community. Key challenges faced by the PCAC have included determining the optimal size and composition of the group, understanding the complex structure of the network as a whole, coordinating with other network entities and functions, and integrating the patient and community voice into the research review process. Efforts to engage stakeholders in clinical data research networks should anticipate and develop solutions to address these challenges.
Oxidized Cashew Gum Scaffolds for Tissue Engineering
Authors: Maciel Jeanny S. , Azevedo Sara , Correia Clara R. , Costa Ana M. S. , Costa Rui R. , Magalhães Francisco A. , de Sousa Monteiro Aliny Abreu , Costa José Francisco Gomes , de Paula Regina C. M , Feitosa Judith P. A. , Mano João F. 
Published: January 2019

AbstractIn the last few years, several strategies have been proposed to fabricate scaffolds for tissue engineering (TE) applications; however, they are based on harsh and time‐consuming techniques. The choice for natural polymers such as cashew gum (CG) allows to circumvent the demands of biocompatibility and degradability of TE systems. In this work, CG, a polysaccharide derived fromAnacardium occidentaletrees, is functionalized with aldehyde groups through periodate oxidation. The resultant oxidized cashew gum (CGO) is mixed with gelatin (GE) to yield a covalently crosslinked hydrogel. CGO/GE sponges are obtained by employing a freeze‐drying methodology to the previously obtained hydrogels. The mechanical properties, swelling ability, and porosity of the GE/CGO sponges are tuned by using CGO with different degrees of oxidation. The resultant sponges can maintain high levels of water absorption and recover their initial mechanical properties after cyclic compression. Moreover, these porous and mechanically robust devices can support the adhesion and proliferation of cells, which can envision their application for the regeneration of soft tissues.
Brand love: Emotionality and development of its elements across the relationship lifecycle
Authors: Schmid David A. , Huber Frank 
Published: January 2019

AbstractBrand love is the most emotionally intense connection between consumers and their brands. Referring to their interpersonal counterpart, it is assumed that consumer–brand relationships change their nature over time. However, most studies in this field do not take into consideration this dynamic characteristic. As part of a dynamic perspective of emotionality within consumer–brand relationships, this study is the first to integrate traditional relationship lifecycle concepts and interpersonal love theories. Addressing the developmental path of brand love dimensions over the course of a relationship, the analysis shows that the emotional bond with a brand as represented by brand love and its subdimensions do not follow a prototypical trajectory as assumed by traditional lifecycle concepts. Instead of forming an inverse U‐shape, the respective dimensions show divergent paths rather resembling the development of their interpersonal correspondent. Comparing hedonic and utilitarian brand concepts, it is shown that brand love can arise for hedonic brands and, just as well, for brands perceived as predominantly utilitarian. Moreover, the results advocate the singularity of brand love among relational constructs, thereby paving the way for a distinct theoretical approach centering on the lifecycle of love. Theoretical and managerial implications are discussed.
Under the influence of a blogger: The role of information‐seeking goals and issue involvement
Authors: Balabanis George , Chatzopoulou Elena 
Published: January 2019

AbstractBloggers in the postmodern era can influence the behaviour of consumers and alter their perceptions. The current study looks at the blogger influence phenomenon from a different theoretical perspective than existing literature did. By combining information behaviour and communication theory, it proposes a new conceptual framework by relying on the characteristics of the blogger, the blog content, motivation, and goals of information seeker. In this model, information seekers’ objectives and issue the involvement are important drivers of blog selection and determinants of the blog's influence. The framework was empirically tested in a sample of American users of beauty blogs. The results provide a confirmation of the postulated relationships. Three types of blog visits are identified and their effects and their attributes that make a blog influential are examined.
Earlier Dopaminergic Treatment in Parkinson's Disease Is Not Associated With Improved Outcomes
Authors: Schneider Ruth B. , Lu Xiang , Biglan Kevin , McDermott Michael P. 
Published: January 2019

ABSTRACTBackgroundThe appropriate timing of dopaminergic treatment initiation in Parkinson's disease (PD) remains a matter of debate. The primary objective of this study was to determine whether earlier initiation of treatment was associated with less worsening of total UPDRS scores over 48 months.MethodsWe performed a secondary analysis of data from the CALM‐PD (Comparison of the Agonist Pramipexole With Levodopa on Motor Complications of Parkinson's Disease) trial to examine the associations between years since diagnosis and 48‐month changes in total and component UPDRS scores, Parkinson's Disease Quality of Life Scale (PDQUALIF) score, and the EuroQol‐5D visual analogue scale (VAS) score.ResultsThere were no associations between years since PD diagnosis and 48‐month changes in total UPDRS, component UPDRS scores, PDQUALIF score, or EuroQol‐5D VAS score.ConclusionEarlier treatment was not associated with improved long‐term outcomes in this secondary analysis. Prospective studies are required to determine the appropriate timing of initiation of dopaminergic treatment to inform clinical practice.
Genetic analysis of the relation of telomere length‐related gene (RTEL1) and coronary heart disease risk
Authors: Lu Shijuan , Zhong Jianghua , Wu Miao , Huang Kang , Zhou Yilei , Zhong Zanrui , Li Qiang , Zhou Honghao 
Published: January 2019

AbstractBackgroundRegulator of telomere elongation helicase 1 (RTEL1), a telomere length‐related gene, is closely linked to cancer and age‐related diseases. The aim of this study was to investigate the association between genetic polymorphisms in the RTEL1 gene and coronary heart disease (CHD) risk.MethodsIn this case–control study, which includes samples from 596 CHD patients and 603 healthy controls, five SNPs in RTEL1 were selected. The genotypes were studied using the Agena MassARRAY platform, and the statistical analyses were performed using the chi‐square and Fisher's exact tests, genetic model analysis, and haplotype analysis.ResultsIn the allele model, using the chi‐square test, we found that the patients with the “G” allele of rs6010620 and the “C” allele of rs4809324 in the RTEL1 gene showed a decreased risk of CHD once the results were adjusted for age and gender. In the genetic model, logistic regression analyses revealed that the rs6010620 polymorphism conferred a decreased risk of CHD in the codominant model (OR = 0.52, 95% CI: 0.31–0.88, p = 0.007 for the “G/G” genotype) and the recessive model (OR = 0.49, 95% CI: 0.30–0.80, p = 0.004 for the “G/G” genotype). In addition, the haplotype “Grs6010620Trs6010621Trs4809324” of RTEL1 was associated with a 0.03‐fold decreased risk of CHD once the results were adjusted for age and gender (OR = 0.03, 95% CI: 0.01–0.12, p < 0.001).ConclusionOur findings have demonstrated that the genetic variants of RTEL1 may have a protective role against CHD risk.
Forkhead box O3 promotes colon cancer proliferation and drug resistance by activating MDR1 expression
Authors: Gao Zhuanglei , Li Zhaoxia , Liu Yuelin , Liu Zhonghao 
Published: January 2019

AbstractBackgroundGlobally, colon cancer (CC) is the third reason of tumor‐related deaths. Previous reports indicate that Forkhead box O3 (FOXO3) is involved in the development of various tumors and may have different effects depending upon the types of tumors. Hence, this study was to examine the effects of FOXO3 on CC cells and uncover the possible mechanisms.MethodsMTT and cell count assay were applied to analyze the viability of transfected CC cells. rVista, dual luciferase reporter assay, and chromatin immunoprecipitation assay were used to identify the downstream target of FOXO3 in HCT116 cells. The mRNA and protein abundance of FOXO3 and MDR1 were determined by quantitative PCR and Western blot, respectively.ResultsForkhead box O3 stimulated the proliferation of both HCT116 and DLD1 cells. Moreover, FOXO3 overexpression inhibited doxorubicin sensitivity of HCT116 cells, while the knockout of FOXO3 by FOXO3 shRNA restored the doxorubicin sensitivity in doxorubicin‐resistant HCT116 DR cells. Next, we found that FOXO3 directly bound to the promoter of MDR1 and enhanced MDR1 expression in HCT116 cells. MDR1 overexpression enhanced the viability and doxorubicin resistance of CC cells. Besides, MDR1 overexpression plasmid significantly abrogated the decrease in cell proliferation and resistance of HCT116 cells to doxorubicin caused by FOXO3 knockout.ConclusionForkhead box O3 exhibited promotive effects on the proliferation and doxorubicin resistance in CC cells via targeting MDR1.
Introduction to the special issue: The WHO World Mental Health International College Student (WMH‐ICS) initiative
Authors: Cuijpers Pim , Auerbach Randy P. , Benjet Corina , Bruffaerts Ronny , Ebert David , Karyotaki Eirini , Kessler Ronald C. 
Published: January 2019

AbstractMost mental disorders have their first onset in early adulthood. Epidemiological research, as well as research on preventive and early interventions, is therefore very important. This thematic issue focuses on one of the first systematic attempts to develop such services for college students. The WHO World Mental Health International College Student (WMH‐ICS) initiative is based on the largest and continuously growing epidemiological dataset ever collected in college students. Based on these results, the initiative has now started to implement internet‐based interventions for common mental disorders and emotional problems. In this special issue, a general paper about the initiative is presented, as well as a paper on the implementation of the WMH‐ICS initiative in low and middle income countries. It also includes several papers with core epidemiological results of the initiative, a meta‐analysis of internet‐based interventions for mental health problems in college students and the first results of trials conducted as part of the initiative. Taken together, the papers in this special issue show that WMH‐ICS is on its way to becoming a major initiative in addressing the problem of unmet need for treatment of mental health problems among college students.
Characterization of gluten‐free bread crumb baked at atmospheric and reduced pressures using TD‐NMR
Authors: Rondeau‐Mouro Corinne , Godfrin Célia , Cambert Mireille , Rouillac Judicaël , Diascorn Yves , Lucas Tiphaine , Grenier David 
Published: January 2019

AbstractThis research aimed to study the effects of using a partial vacuum for bread baking on macromolecules and water distribution in gluten‐free bread. Bread baking under partial vacuum results in greater oven rise and a larger gas fraction in the crumb. Because water's boiling point decreases under reduced pressure, it was expected that its distribution within the dough and its interactions with the others dough's constituents (mainly starch) would differ from those in bread baked under atmospheric pressure. Time‐domain nuclear magnetic resonance was used, as it has the rare capacity to quantify both gelatinization and retrogradation of starch. Complementary rheological measurements made it possible to show that crumb Young's modulus was mostly influenced by the gas fraction whereas there was little change in starch gelatinization and retrogradation when dough was baked under partial vacuum. When insufficiently hydrated (48%), the volume of breads was practically the same whatever the baking process. Meanwhile, the nuclear magnetic resonance results suggested that amylose short‐term crystallization (on cooling) is dependent on water content. In addition, crumb Young's modulus during storage at room temperature correlated with an increase in free induction decay signal intensity.
Sohlh1 is required for synaptonemal complex formation by transcriptionally regulating meiotic genes during spermatogenesis in mice
Authors: Li Yuan , Qi Wanjing , Liu Gongqing , Du Bing , Sun Qi , Zhang Xue , Jin Meiyu , Dong Wanwei , Liu Jia , Zheng Zhihong 
Published: January 2019

AbstractGonad‐specific transcription factor spermatogenesis‐ and oogenesis‐specific helix‐loop‐helix transcription factor 1 (SOHLH1) plays a key role in the transcriptional regulation of the expression of differentiating spermatogonial genes. However, its role in spermatocytes (meiotic male germ cells) remains largely unknown. In this study, Sohlh1 knockout (KO) male mice displayed meiotic defects at the zygotene stage during spermatogenesis. Microarray analyses identified 66 upregulated genes and 139 downregulated genes in Sohlh1 KO testes compared with those in wild‐type testes at postnatal Day 7.5. Among many of the downregulated genes, Sycp1 and Sycp3, which encode synaptonemal complex proteins 1 and 3 (SYCP1 and SYCP3), respectively, were significantly reduced in Sohlh1 knockout mice. Transmission electron microscopy revealed no formation of the synaptonemal complex in Sohlh1 KO spermatocytes. Luciferase reporter and chromatin‐immunoprecipitation assays demonstrated that SOHLH1 enhanced the expression of the Sycp1 and Sycp3 genes by binding the −1276, −708, and −94 basepairs (bp) E‐boxes upstream of the Sycp1 promoter and the −64 and −43 bp E‐boxes upstream of the Sycp3 promoter. Our data suggest that SOHLH1 transcriptionally regulates the expression of many target genes critical for the meiotic phase of spermatogenesis.