bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic value of clinical and radiological findings for conservative treatment of idiopathic ulnar impaction syndrome
Authors: Roh Young Hak , Kim Sangwoo , Gong Hyun Sik , Baek Goo Hyun 
Published: June 2018

AbstractUlnar impaction syndrome (UIS) is a common source of ulnar-sided wrist pain, yet not all cases of radiographic ulnar impaction are symptomatic. We retrospectively analyze clinical or radiologic factors that affect prognoses of conservative treatment for idiopathic UIS. A total of 114 patients who had been diagnosed with UIS were treated with 6 weeks of short arm orthosis followed by formal physiotherapy for 6 weeks, with lifestyle modification to limit aggravating movements. The response to treatment, including pain numeric rating scale on an ulnar provocation test, grip strength, Disability of the Arm, Shoulder, and Hand score (DASH), was assessed at 24-week follow-up. For the 24-week follow-up, 29 patients (25%) underwent ulnar shortening osteotomy due to persistent symptoms after conservative treatment, and 18 (16%) patients had pain scores of greater than 5, but they had not undergone surgery. After controlling for confounding variables, female gender (odds ratio (OR) 1.39), duration of symptom (OR 1.27), high pain NRS score on provocation test (OR 1.45), and enhanced carpal or distal ulna bone on MRI (OR 1.82) were associated with a higher likelihood of treatment failure. Knowledge of the factors offers physicians insight into predicting prognoses and helps patients set realistic expectations.
The Open Source GAITOR Suite for Rodent Gait Analysis
Authors: Jacobs Brittany Y. , Lakes Emily H. , Reiter Alex J. , Lake Spencer P. , Ham Trevor R. , Leipzig Nic D. , Porvasnik Stacy L. , Schmidt Christine E. , Wachs Rebecca A. , Allen Kyle D. 
Published: June 2018

AbstractLocomotive changes are often associated with disease or injury, and these changes can be quantified through gait analysis. Gait analysis has been applied to preclinical studies, providing quantitative behavioural assessment with a reasonable clinical analogue. However, available gait analysis technology for small animals is somewhat limited. Furthermore, technological and analytical challenges can limit the effectiveness of preclinical gait analysis. The Gait Analysis Instrumentation and Technology Optimized for Rodents (GAITOR) Suite is designed to increase the accessibility of preclinical gait analysis to researchers, facilitating hardware and software customization for broad applications. Here, the GAITOR Suite’s utility is demonstrated in 4 models: a monoiodoacetate (MIA) injection model of joint pain, a sciatic nerve injury model, an elbow joint contracture model, and a spinal cord injury model. The GAITOR Suite identified unique compensatory gait patterns in each model, demonstrating the software’s utility for detecting gait changes in rodent models of highly disparate injuries and diseases. Robust gait analysis may improve preclinical model selection, disease sequelae assessment, and evaluation of potential therapeutics. Our group has provided the GAITOR Suite as an open resource to the research community at www.GAITOR.org, aiming to promote and improve the implementation of gait analysis in preclinical rodent models.
Variability in venom composition of European viper subspecies limits the cross-effectiveness of antivenoms
Authors: Zanetti Giulia , Duregotti Elisa , Locatelli Carlo Alessandro , Giampreti Andrea , Lonati Davide , Rossetto Ornella , Pirazzini Marco 
Published: June 2018

AbstractMedically relevant cases of snakebite in Europe are predominately caused by European vipers of the genus Vipera. Systemic envenoming by European vipers can cause severe pathology in humans and different clinical manifestations are associated with different members of this genus. The most representative vipers in Europe are V. aspis and V. berus and neurological symptoms have been reported in humans envenomed by the former but not by the latter species. In this study we determined the toxicological profile of V. aspis and V. berus venoms in vivo in mice and we tested the effectiveness of two antivenoms, commonly used as antidotes, in counteracting the specific activities of the two venoms. We found that V. aspis, but not V. berus, is neurotoxic and that this effect is due to the degeneration of peripheral nerve terminals at the NMJ and is not neutralized by the two tested antisera. Differently, V. berus causes a haemorrhagic effect, which is efficiently contrasted by the same antivenoms. These results indicate that the effectiveness of different antisera is strongly influenced by the variable composition of the venoms and reinforce the arguments supporting the use polyvalent antivenoms.
A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures
Authors: Septiadi D. , Bourquin J. , Durantie E. , Petri-Fink A. , Rothen-Rutishauser B. 
Published: June 2018

AbstractThree dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor kinetics and dynamics of particle uptake by cells and translocation across tissue membranes.
Exposure of Methicillin-Resistant Staphylococcus aureus to Low Levels of the Antibacterial THAM-3ΦG Generates a Small Colony Drug-Resistant Phenotype
Authors: Weaver Alan J. , Peters Tami R. , Tripet Brian , Van Vuren Abigail , Rakesh  , Lee Richard E. , Copié Valérie , Teintze Martin 
Published: June 2018

AbstractThis study investigated resistance against trishexylaminomelamine trisphenylguanide (THAM-3ΦG), a novel antibacterial compound with selective microbicidal activity against Staphylococcus aureus. Resistance development was examined by culturing methicillin resistant S. aureus (MRSA) with sub-lethal doses of THAM-3ΦG. This quickly resulted in the formation of normal (WT) and small colonies (SC) of S. aureus exhibiting minimal inhibitory concentrations (MICs) 2× and 4× greater than the original MIC. Continuous cell passaging with increasing concentrations of THAM-3ΦG resulted in an exclusively SC phenotype with MIC >64 mg/L. Nuclear magnetic resonance (NMR)-based metabolomics and multivariate statistical analysis revealed three distinct metabolic profiles for THAM-3ΦG treated WT, untreated WT, and SC (both treated and untreated). The metabolome patterns of the SC sample groups match those reported for other small colony variants (SCV) of S. aureus. Supplementation of the SCV with menadione resulted in almost complete recovery of growth rate. This auxotrophism was corroborated by NMR analysis revealing the absence of menaquinone production in the SCV. In conclusion, MRSA rapidly acquires resistance to THAM-3ΦG through selection of a slow-growing menaquinone auxotroph. This study highlights the importance of evaluating and monitoring resistance to novel antibacterials during development.
Nitrogen loading effects on nitrification and denitrification with functional gene quantity/transcription analysis in biochar packed reactors at 5 °C
Authors: He Su , Ding Lili , Pan Yao , Hu Haidong , Ye Lin , Ren Hongqiang 
Published: June 2018

AbstractThis study investigated the nitrogen transformation rates of different nitrogen-loading (20, 30, and 50 mg TN/L) biochar packed reactors (C:N:P = 100:5:1) within 125 days at 5 °C. The results showed that high nitrogen loading resulted in an NH4+ (TN) removal efficiency decline from 98% (57%) to 83% (29%), with biochar yielding a higher NH4+, TN and DON removal rate than conventional activated sludge. Moreover, all biochar packed reactors realized a quick start-up by dropping in temperature stage by stage, and the effluent dissolved organic nitrogen (DON) concentrations of R20, R30, and R50 were 0.44 ± 0.18, 0.85 ± 0.35, and 0.66 ± 0.26 mg/L, respectively. The nirS/amoA, nxrA/amoA, and amoA/(narG + napA) were deemed to be the markers of ammonium oxidation rate (SAOR), specific nitrite oxidation rate (SNOR), and specific nitrate reduction rate (SNRR), respectively. Compared with functional gene quantity data, transcription data (mRNA) introduced into stepwise regression analyses agreed well with nitrogen transformation rates. High nitrogen loading also resulted in the cell viability decreased in R50. Nitrogen loadings and operation time both led to a significant variation in cell membrane composition, and unsaturated fatty acids (UFAs) significantly increased in R30 (46.49%) and R50 (36.34%). High-throughput sequencing revealed that nitrogen loadings increased the abundance of nitrifying bacteria (e.g., Nitrospira) and reduced the abundance of denitrifying bacteria (e.g., Nakamurella, Thermomonas, and Zoogloea) through linear discriminant analysis (LDA).
BK channel deacetylation by SIRT1 in dentate gyrus regulates anxiety and response to stress
Authors: Yu Diankun , Homiack Damek R. , Sawyer Edward J. , Schrader Laura A. 
Published: June 2018

AbstractPrevious genomic studies in humans indicate that SIRT1, a nicotinamide adenine dinucleotide (NAD+)-dependent protein deacetylase, is involved in anxiety and depression, but the mechanisms are unclear. We previously showed that SIRT1 is highly activated in the nuclear fraction of the dentate gyrus of the chronically stressed animals and inhibits memory formation and increases anhedonic behavior during chronic stress, but specific functional targets of cytoplasmic SIRT1 are unknown. Here, we demonstrate that SIRT1 activity rapidly modulates intrinsic and synaptic properties of the dentate gyrus granule cells and anxiety behaviors through deacetylation of BK channel α subunits in control animals. Chronic stress decreases BKα channel membrane expression, and SIRT1 activity has no rapid effects on synaptic transmission or intrinsic properties in the chronically stressed animal. These results suggest SIRT1 activity rapidly modulates the physiological function of the dentate gyrus, and this modulation participates in the maladaptive stress response.
The Role of Cognition on Navigational Skills of Children and Adolescents With Autism Spectrum Disorders
Authors: Robillard Manon , Roy-Charland Annie , Cazabon Sylvie 
Published: June 2018

PurposeThis study examined the role of cognition on the navigational process of a speech-generating device (SGD) among individuals with a diagnosis of autism spectrum disorder (ASD). The objective was to investigate the role of various cognitive factors (i.e., cognitive flexibility, sustained attention, categorization, fluid reasoning, and working memory) on the ability to navigate an SGD with dynamic paging and taxonomic grids in individuals with ASD.MethodTwenty individuals aged 5 to 20 years with ASD were assessed using the Leiter International Performance Scale–Revised (Roid & Miller, 1997) and the Automated Working Memory Assessment (Alloway, 2007). They also completed a navigational task using an iPad 4 (Apple, 2017; taxonomic organization).ResultsSignificant correlations between all of the cognitive factors and the ability to navigate an SGD were revealed. A stepwise linear regression suggested that cognitive flexibility was the best predictor of navigational ability with this population.ConclusionThe importance of cognition in the navigational process of an SGD with dynamic paging in children and adolescents with ASD has been highlighted by the results of this study.
Coexistence of magneto-rotational and Jeans instabilities in an axisymmetric nebula
Authors: Montani Giovanni , Palermo Giovanni , Carlevaro Nakia 
Published: June 2018

Aims. We analyze the magneto-rotational instability (MRI) effects on gravitational collapse and its influence on the instability critical scale.
Methods. In particular, we study an axisymmetric nonstratified differentially rotating cloud, embedded in a small magnetic field, and we perform a local linear stability analysis, including the self gravity of the system.
Results. We demonstrate that the linear evolution of the perturbations is characterized by the emergence of an anisotropy degree of the perturbed mass densities. Starting with spherical growing overdensities, we see that they naturally acquire an anisotropy of order unity in their shape. Despite the linear character of our analysis, we infer that such a seed of anisotropy can rapidly grow in a nonlinear regime, leading to the formation of filament-like structures. However, we show how such an anisotropy is essentially an intrinsic feature of the Jean instability, and how MRI only plays a significant role in fixing the critical scale of the mode spectrum. We then provide a characterization of the present analysis in terms of the cosmological setting, in order to provide an outlook of how the present results could concern the formation of large-scale structures across the Universe.
Low-mass star formation and subclustering in the H II regions RCW 32, 33, and 27 of the Vela Molecular Ridge
Authors: Prisinzano L. , Damiani F. , Guarcello M. G. , Micela G. , Sciortino S. , Tognelli E. , Venuti L. 
Published: June 2018

Context. Most stars are born in clusters, and recent results suggest that star formation (SF) preferentially occurs in subclusters. Studying the morphology and SF history of young clusters is crucial for understanding early cluster formation processes.
Aims. We aim to identify the embedded population of young stellar objects (YSOs) down to the low-mass stars in the M-type regime in the three H II regions RCW 33, RCW 32, and RCW 27, which are located in the northwestern region of the Vela Molecular Ridge. Our aim is to characterize their properties, such as morphology and extent of the clusters in the three H II regions, derive stellar ages, and determine the connection of the SF history with the environment.
Methods. Through public photometric surveys such as Gaia, VPHAS+, 2MASS, and Spitzer/GLIMPSE, we identify YSOs with classical techniques aimed at detecting IR, Hα, and UV excesses as signatures of circumstellar disks and accretion. In addition, we implement a method for distinguishing main-sequence (MS) stars and giants in the M-type regime by comparing the reddening derived in several optical/IR color-color diagrams, assuming suitable theoretical models. Since this diagnostic is sensitive to stellar gravity, the procedure allows us to also identify pre-MS (PMS) stars.
Results. Using the classical membership criteria, we find that a large population of YSOs shows signatures of circumstellar disks with or without accretion. In addition, with the new technique of M-type star selection, we find a rich population of young M-type stars whose spatial distribution strongly correlates with the more massive population. We find evidence of three young clusters, with different morphology, for which we estimate the individual distances using TGAS Gaia data of the brighter subsample. In addition, we identify field stars falling in the same region by securely classifying them as giants and foreground MS stars.
Conclusions. We identify the embedded population of YSOs down to about 0.1 M⊙ that is associated with the three H II regions RCW 33, RCW 32, and RCW 27 and the three clusters Vela T2, Cr 197, and Vela T1, respectively. All the three clusters are located at a similar distance, but they have very different morphologies. Our results suggest a decreasing SF rate in Vela T2 and triggered SF in Cr 197 and Vela T1.
Der Kaiserschnitt – Sectio caesarea
Authors: Wagenhäuser Karl-Heinz 
Published: June 2018

ZusammenfassungDer Kaiserschnitt wurde früher fast ausschließlich aus medizinischen Gründen durchgeführt, wenn eine Geburt durch die Scheide für Mutter oder Kind zu gefährlich
erschien. Heutzutage wird etwa jedes dritte Kind in Deutschland per Kaiserschnitt entbunden, wobei es große regionale Unterschiede gibt. Der starke Anstieg auf
durchschnittlich fast 32 Prozent wird kontrovers diskutiert. Wir stellen Ihnen Schritt für Schritt die Sectio caesarea vor.
Attraktive Pflege
Authors: König Anja 
Published: June 2018

ZusammenfassungDas Projekt „Heidelberger Expertise in der Pflege“ – kurz HEP 2020 – hat sich zum Ziel gesetzt, die Arbeitsbedingungen im Pflegeberuf zu verbessern, um
qualifizierte Mitarbeiter zu rekrutieren und dauerhaft zu binden, die Berufszufriedenheit zu erhöhen und eine exzellente Patientenversorgung sicherzustellen.
Wir stellen Ihnen das Projekt vor.
Interdisziplinäres Projekt: Lagerungsleitlinie
Authors: Möller Franziska 
Published: June 2018

ZusammenfassungEin gemeinsames Projekt schweißt das Team zusammen und erhöht die Mitarbeiterbindung. Deshalb wurde am Universitätsklinikum Heidelberg mit allen Beteiligten eine
interdisziplinäre Lagerungsleitlinie erarbeitet. Durch die enge Zusammenarbeit beim Erstellen der Leitlinie hat sich die Kommunikation zwischen den
Berufsgruppen verbessert. Zusätzlich erhöhte sich die Mitarbeiterzufriedenheit, da es nun ein einheitliches Vorgehen bei der Lagerung gibt und jeder weiß, was
er zu tun hat.
Giant Prolactinomas: Outcomes of Multimodal Treatments for 42 Cases with Long-Term Follow-Up
Authors: Lv Liang , Hu Yu , Yin Senlin , Zhou Peizhi , Yang Yuan , Ma Weichao , Zhang Shizhen , Wang Xiujie , Jiang Shu 
Published: June 2018

AbstractGiant prolactinomas represent a rare entity of pituitary tumors so that the management of these patients is still a prevalent challenge at present. Paying special attention to the treatment strategy and outcomes, we presented a large series of 42 cases looking forward to share our understanding and experience in management of these patients. Male patients accounted for 71.4% of this series and were relatively younger (35.70±2.42 vs. 52.00±3.55 years, p=0.0011) and harbored bigger tumors (14.57 vs. 7.74 cm3, p=0.0179) compared to females. Almost all of these tumors showed suprasellar extension (97.6%) and cavernous sinus invasion (92.9%). Dopamine agonist represented an efficient method to control PRL concentrations (98.8%) and reduce tumor burdens (81.2 %). PRL normalization was detected in 13 out of the 27 patients initially treated with bromocriptine (BRC) whereas none of the 14 patients with first-line operation gained a normalization of PRL concentration after surgery. Although there was no reliable predictor of tumor response, First PRL reduction was a predictive criterion for the nadir PRL level during the long-time period of follow-up for first-line bromocriptine treatment. In conclusion, patients with giant prolactinomas did not gain more benefits from initial surgery. Dopamine agonist (BRC) should be first-line treatment for giant prolactinomas whereas operation merely served as a remedy for acute compression symptoms and dopamine agonist resistance. Consecutive monitoring of serum PRL levels in the early stage of initial BRC treatment is useful for evaluation of therapeutic effect and further therapeutic decision.
Einflussfaktoren auf kosmetische Langzeitergebnisse nach minimalinvasiver videoassistierter Thyreoidektomie und konventioneller Schilddrüsenoperation
Authors: Sahm Maik , Otto Ronny , Pross Matthias , Mantke Rene 
Published: June 2018

Zusammenfassung
Hintergrund Circa 90 000 Schilddrüsenoperationen werden jährlich in Deutschland durchgeführt. 5 – 10% der Operationen werden in Zentren als minimalinvasive videoassistierte Thyreoidektomie (MIVAT) durchgeführt. Es gibt nur wenige Daten über die kosmetischen Langzeitergebnisse nach MIVAT im Vergleich zur konventionellen Operation. Aktuelle systematische Reviews zeigen keinen Vorteil der MIVAT. Ziel dieser Studie war die Analyse der postoperativen Langzeitergebnisse beider Prozeduren und die Beurteilung der beeinflussenden Faktoren.
Patienten und Methode Die Analyse der Langzeitergebnisse basiert auf Nachuntersuchungen mit einem validierten Narbenbegutachtungsscore (POSAS). Die Kohortenanalyse bezieht sich auf die MIVAT-Operationen der Klinik von 2004 bis 2011 sowie die Patienten mit konventioneller Schilddrüsenoperation des Jahres 2011.
Ergebnisse In der MIVAT-Gruppe wurden die Nachuntersuchungsdaten von 117 Patienten und in der konventionellen Gruppe von 102 Patienten ausgewertet. Das Follow-up betrug 23,1 vs. 23,6 Monate (MIVAT vs. konv. Gruppe). Im Patientenscore ist ein Vorteil der konventionellen Gruppe nachweisbar. Der Friedman-Test zeigte mit der Pigmentation (mittlerer Rang 4,79) und dem Relief einer Narbe (mittlerer Rang 3,62) die wesentlichen Faktoren, die das kosmetische Langzeitergebnis beeinflussen.
Schlussfolgerung Die MIVAT und die konventionelle Operation zeigen in den kosmetischen Langzeitergebnissen sehr gute Ergebnisse. Von Patientenseite ist ein nicht signifikanter Vorteil der konventionellen Operation nachweisbar. Chirurgisch nur bedingt beeinflussbare Faktoren, wie Narbenpigmentation und das Relief, wirken wesentlich in die Bewertung der kosmetischen Langzeitergebnisse ein.
Inactivation of Antiplasmin and Complement C1 in Human Plasma Rendered Fibrinolytic by Synthetic Organic Compounds
Authors: von Kaulla Kurt N 
Published: June 2018

SummaryCertain synthetic organic compounds induce upon dissolution marked fibrinolytic activity in human plasma, reduce the antiplasmin titer of human or bovine serum and destroy the complement C1 of human plasma. Generation of fibrinolytic activity and reduction of antiplasmin are concentration-depending time reactions. Destruction of complement C1 occurs almost instantaneously. Minor molecular modifications abolish all three activities of the compounds.
Evaluation of Activity of Phospholipids in Blood Coagulation in Vitro
Authors: Silver M. J , Turner D. L , Rodalewicz I , Giordano N , Holburn R , Herb S. F , Luddy Francis E 
Published: June 2018

Summary1. The effects of phospholipids, solubilized by different methods in different clotting tests are presented.2. Complete fatty acid analysis of a phosphatidylserine fraction from beef brain is given.3. Data relating electric charge of phospholipid particles to activity in clotting are presented.4. A critical discussion of the factors which influence the activity of phospholipids in blood clotting terminates the paper.
Moderate Intravascular Hemolysis and Experimental Venous Thrombosis in Rats
Authors: Nordöy Arne 
Published: June 2018

Summary and ConclusionsModerate intravascular hemolysis was produced in rats by intraperitoneal injections of hemolysate prepared from rats.1.	An accelerated clotting time of recalcified plasma and an increased platelet adhesiveness were observed during the first ten hours after the injection.2.	An increase of the fibrinogen and proaccelerin level of plasma were observed 24—48 hours after the injection.3.	No increased antifibrinolytic activity was found.4.	No significant increase in the incidence of thrombosis was observed during the first 24 hours after the injection whereas a high incidence of thrombosis indicating a hyperthrombotic state was observed 24—48 hours after the injection, at a time when the hemoglobinemia had disappeared. In a group of animals given a second injection of hemolysate 24 hours after the first injection a hyperthrombotic state was still present 25—48 hours after the first injection with an incidence of thrombosis equal to that noted 24—48 hours after a single injecion only.5.	The present study may indicate that the hyperthrombotic state present during moderate intravascular hemolysis is associated with an increased formation of fibrinogen, factor V (proaccelerin) and probably factor VIII (AHG).