bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imprint of Holocene Climate Variability on Cold‐Water Coral Reef Growth at the SW Rockall Trough Margin, NE Atlantic
Authors: Bonneau Lucile , Colin Christophe , Pons‐Branchu Edwige , Mienis Furu , Tisnérat‐Laborde Nadine , Blamart Dominique , Elliot Mary , Collart Tim , Frank Norbert , Foliot Lorna , Douville Eric 
Published: May 2018

AbstractU‐Th ages and temperatures derived from Li/Mg have been measured on coral fragments of Lophelia pertusa and Madrepora oculata collected from two sediment cores, which were taken from cold‐water coral (CWC) mounds at 700–790m water depth at the SW Rockall Trough margin. Our data, combined with previous published data, have allowed us to first estimate the occurrence of CWC at the SW Rockall Trough margin during the Holocene and, second, to better constrain the environmental conditions driving variability in CWC growth. CWC abundance is marked by a pronounced increase in the mid‐Holocene (∼6 ka) and is modulated by millennial‐scale variability throughout the late‐Holocene. The mid‐Holocene proliferation of CWC coincides with lowest IRD abundances and a major reorganization of the circulation at thermocline depth in the Rockall Trough, marked by the progressive replacement of the fresh‐cold Sub‐Arctic Intermediate Water (SAIW) by the saltier and nutrient‐rich Eastern North Atlantic Water (ENAW). This event must have established a modern‐like winter mixed layer and thermocline structure, generating suitable conditions for enhanced surface productivity, downslope transport of food particles, bottom current acceleration at mound depth and thus CWC growth. Several short time intervals of decreased CWC occurrences closely match prominent increases in North Atlantic drift ice and storminess in Northern Europe. We, therefore, propose that high detrital supply and/or changes in the vertical density gradient associated with millennial‐scale ice‐rafted detritus (IRD) events are the likely controlling factors for CWC growth and subsequent mound formation on the SW Rockall Trough margin.
Constraints on the Source of the Martian Magnetic Anomalies Inferred From Relaxation Time of Remanent Magnetization
Authors: Sato M. , Yamamoto Y. , Nishioka T. , Kodama K. , Mochizuki N. , Ushioda M. , Nakada R. , Tsunakawa H. 
Published: May 2018

AbstractThe strong magnetic anomalies observed over Mars originate from remanence of deep crust, which should be preserved during 4 billion years. We estimate the relaxation time of remanence carried by various types of magnetite, considering temperature and pressure dependences of magnetic parameters. The estimation shows that acicular‐shaped single‐domain magnetite is needed to retain the thick magnetized layer during 4 billion years in a low‐Urey ratio scenario, whereas acicular‐shaped single‐domain, equidimensional single‐domain, and pseudo‐single‐domain magnetites can be the source of anomalies in a high‐Urey ratio scenario. In each case, fine‐grained (<10 μm) and preferably high‐aspect‐ratio magnetite in deep crust is needed to explain the observed anomalies.
Hypocenter Migration and Seismicity Pattern Change in the Yamagata‐Fukushima Border, NE Japan, Caused by Fluid Movement and Pore Pressure Variation
Authors: Yoshida Keisuke , Hasegawa Akira 
Published: May 2018

AbstractThe spatiotemporal distributions of hypocenters and temporal change in seismicity patterns were investigated in details for events in the Yamagata‐Fukushima border earthquake swarm, which was a remotely triggered earthquake sequence by the 2011 Tohoku‐Oki earthquake. We relocated the hypocenters by applying the double‐difference location method to differential arrival time data obtained by waveform cross correlations together with the Japan Meteorological Agency catalogue data. The hypocenter distribution obtained clearly shows that the hypocenters are concentrated on several discrete planes and migrate along those planes from deeper to shallower levels instead of diffusing isotropically. Most of the events have nodal planes of focal mechanisms parallel to those discrete planes, suggesting that ruptures of individual events occurred along those macroscopic planes. The timing of earthquake occurrences is almost random during the initial ~50 days, but it gradually becomes temporally clustered in later periods, with the number of events decaying aftershock‐like after relatively large events. These observations suggest that the present swarm is caused by a reduction in frictional strength due to the increased pore pressure of fluids rising from greater depths in response to the decrease in arc‐normal compressional stress associated with the Tohoku‐Oki earthquake. The fluids permeated into several existing planes, reduced frictional strengths, caused the present earthquake swarm, and migrated upward along the planes with the hypocenters. Our previous observations that stress drops are systematically low and b‐values are high during the initial ~50 days can be readily explained if the pore pressure is especially high during that period.
Long‐Lasting Geomagnetically Induced Currents and Harmonic Distortion Observed in New Zealand During the 7–8 September 2017 Disturbed Period
Authors: Clilverd Mark A. , Rodger Craig J. , Brundell James B. , Dalzell Michael , Martin Ian , Mac Manus Daniel H. , Thomson Neil R. , Petersen Tanja , Obana Yuki 
Published: May 2018

AbstractSeveral periods of geomagnetically induced currents (GICs) were detected in the Halfway Bush substation in Dunedin, South Island, New Zealand, as a result of intense geomagnetic storm activity during 6 to 9 September 2017. Unprecedented data coverage from a unique combination of instrumentation is analyzed, that is, measurements of GIC on the single‐phase bank transformer T4 located within the substation, nearby magnetic field perturbation measurements, very low frequency (VLF) wideband measurements detecting the presence of power system harmonics, and high‐voltage harmonic distortion measurements. Two solar wind shocks occurred within 25 hr, generating four distinct periods of GIC. Two of the GIC events were associated with the arrival of the shocks themselves. These generated large but short‐lived GIC effects that resulted in no observable harmonic generation. Nearby and more distant magnetometers showed good agreement in measuring these global‐scale magnetic field perturbations. However, two subsequent longer‐lasting GIC periods, up to 30 min in duration, generated harmonics detected by the VLF receiver systems, when GIC levels continuously exceeded 15 A in T4. Nearby and more distant magnetometers showed differences in their measurements of the magnetic field perturbations at these times, suggesting the influence of small‐scale ionospheric current structures close to Dunedin. VLF receiver systems picked up harmonics from the substation, up to the 30th harmonic, consistent with observed high‐voltage increases in even harmonic distortion, along with small decreases in odd harmonic distortion.
Inferior olive CRF plays a role in motor performance under challenging conditions
Authors: Ezra-Nevo Gili , Volk Naama , Ramot Assaf , Kuehne Claudia , Tsoory Michael , Deussing Jan , Chen Alon 
Published: May 2018

AbstractA well-coordinated stress response is pivotal for an organisms’ survival. Corticotropin-releasing factor (CRF) is an essential component of the emotional and neuroendocrine stress response, however its role in cerebellar functions is poorly understood. Here, we explore the role of CRF in the inferior olive (IO) nucleus, which is a major source of input to the cerebellum. Using a CRF reporter line, in situ hybridization and immunohistochemistry, we demonstrate very high levels of the CRF neuropeptide expression throughout the IO sub-regions. By generating and characterizing IO-specific CRF knockdown and partial IO-CRF knockout, we demonstrate that reduction in IO-CRF levels is sufficient to induce motor deficiency under challenging conditions, irrespective of basal locomotion or anxiety-like behavior. Furthermore, we show that chronic social defeat stress induces a persistent decrease in IO-CRF levels, and that IO-CRF mRNA is upregulated shortly following stressful situations that demand a complex motor response. Taken together our results indicate a role for IO-CRF in challenge-induced motor responses.
Operando monitoring the lithium spatial distribution of lithium metal anodes
Authors: Lv Shasha , Verhallen Tomas , Vasileiadis Alexandros , Ooms Frans , Xu Yaolin , Li Zhaolong , Li Zhengcao , Wagemaker Marnix 
Published: May 2018

AbstractElectrical mobility demands an increase of battery energy density beyond current lithium-ion technology. A crucial bottleneck is the development of safe and reversible lithium-metal anodes, which is challenged by short circuits caused by lithium-metal dendrites and a short cycle life owing to the reactivity with electrolytes. The evolution of the lithium-metal-film morphology is relatively poorly understood because it is difficult to monitor lithium, in particular during battery operation. Here we employ operando neutron depth profiling as a noninvasive and versatile technique, complementary to microscopic techniques, providing the spatial distribution/density of lithium during plating and stripping. The evolution of the lithium-metal-density-profile is shown to depend on the current density, electrolyte composition and cycling history, and allows monitoring the amount and distribution of inactive lithium over cycling. A small amount of reversible lithium uptake in the copper current collector during plating and stripping is revealed, providing insights towards improved lithium-metal anodes.
Uncertainty about social interactions leads to the evolution of social heuristics
Authors: van den Berg Pieter , Wenseleers Tom 
Published: May 2018

AbstractIndividuals face many types of social interactions throughout their lives, but they often cannot perfectly assess what the consequences of their actions will be. Although it is known that unpredictable environments can profoundly affect the evolutionary process, it remains unclear how uncertainty about the nature of social interactions shapes the evolution of social behaviour. Here, we present an evolutionary simulation model, showing that even intermediate uncertainty leads to the evolution of simple cooperation strategies that disregard information about the social interaction (‘social heuristics’). Moreover, our results show that the evolution of social heuristics can greatly affect cooperation levels, nearly doubling cooperation rates in our simulations. These results provide new insight into why social behaviour, including cooperation in humans, is often observed to be seemingly suboptimal. More generally, our results show that social behaviour that seems maladaptive when considered in isolation may actually be well-adapted to a heterogeneous and uncertain world.
SIRT1, miR-132 and miR-212 link human longevity to Alzheimer’s Disease
Authors: Hadar A. , Milanesi E. , Walczak M. , Puzianowska-Kuźnicka M. , Kuźnicki J. , Squassina A. , Niola P. , Chillotti C. , Attems J. , Gozes I. , Gurwitz D. 
Published: May 2018

AbstractAlzheimer’s Disease (AD) is the most common cause of dementia in the elderly. Centenarians – reaching the age of >100 years while maintaining good cognitive skills – seemingly have unique biological features allowing healthy aging and protection from dementia. Here, we studied the expression of SIRT1 along with miR-132 and miR-212, two microRNAs known to regulate SIRT1, in lymphoblastoid cell lines (LCLs) from 45 healthy donors aged 21 to 105 years and 24 AD patients, and in postmortem olfactory bulb and hippocampus tissues from 14 AD patients and 20 age-matched non-demented individuals. We observed 4.0-fold (P = 0.001) lower expression of SIRT1, and correspondingly higher expression of miR-132 (1.7-fold; P = 0.014) and miR-212 (2.1-fold; P = 0.036), in LCLs from AD patients compared with age-matched healthy controls. Additionally, SIRT1 expression was 2.2-fold (P = 0.001) higher in centenarian LCLs compared with LCLs from individuals aged 56–82 years; while centenarian LCLs miR-132 and miR-212 indicated 7.6-fold and 4.1-fold lower expression, respectively. Correlations of SIRT1, miR-132 and miR-212 expression with cognitive scores were observed for AD patient-derived LCLs and postmortem AD olfactory bulb and hippocampus tissues, suggesting that higher SIRT1 expression, possibly mediated by lower miR-132 and miR-212, may protect aged individuals from dementia and is reflected in their peripheral tissues.
Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor
Authors: Schwartz Mathieu , Perrot Thomas , Aubert Emmanuel , Dumarçay Stéphane , Favier Frédérique , Gérardin Philippe , Morel-Rouhier Mélanie , Mulliert Guillermo , Saiag Fanny , Didierjean Claude , Gelhaye Eric 
Published: May 2018

AbstractWood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level. Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones. Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site. These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation.
Inhibitory effects of Myricetin derivatives on curli-dependent biofilm formation in Escherichia coli
Authors: Arita-Morioka Ken-ichi , Yamanaka Kunitoshi , Mizunoe Yoshimitsu , Tanaka Yoshihiko , Ogura Teru , Sugimoto Shinya 
Published: May 2018

AbstractBiofilms are well-organised communities of microbes embedded in a self-produced extracellular matrix (e.g., curli amyloid fibers) and are associated with chronic infections. Therefore, development of anti-biofilm drugs is important to combat with these infections. Previously, we found that flavonol Myricetin inhibits curli-dependent biofilm formation by Escherichia coli (IC50 = 46.2 μM). In this study, we tested activities of seven Myricetin-derivatives to inhibit biofilm formation by E. coli K-12 in liquid culture. Among them, only Epigallocatechin gallate (EGCG), a major catechin in green tea, inhibited biofilm formation of K-12 (IC50 = 5.9 μM) more efficiently than Myricetin. Transmission electron microscopy and immunoblotting analyses demonstrated that EGCG prevented curli production by suppressing the expression of curli-related proteins. Quantitative RT-PCR analysis revealed that the transcripts of csgA, csgB, and csgD were significantly reduced in the presence of EGCG. Interestingly, the cellular level of RpoS, a stationary-phase specific alternative sigma factor, was reduced in the presence of EGCG, whereas the rpoS transcript was not affected. Antibiotic-chase experiments and genetic analyses revealed that EGCG accelerated RpoS degradation by ATP-dependent protease ClpXP in combination with its adaptor RssB. Collectively, these results provide significant insights into the development of drugs to treat chronic biofilm-associated infections.
Molecular basis for dengue virus broad cross-neutralization by humanized monoclonal antibody 513
Authors: Wong Yee Hwa , Kumar Akshita , Liew Chong Wai , Tharakaraman Kannan , Srinivasaraghavan Kannan , Sasisekharan Ram , Verma Chandra , Lescar Julien 
Published: May 2018

AbstractDengue is a widespread viral disease with 3.6 billion people at risk worldwide. Humanized monoclonal antibody (mAb) 513, currently undergoing clinical trials in Singapore, targets an epitope on the envelope protein domain III exposed at the surface of the viral particle. This antibody potently neutralizes all four dengue virus serotypes in a humanized mouse model that recapitulates human dengue infection, without signs of antibody-mediated enhancement of the disease. The crystal structure of single-chain variable fragment (scFv) 513 bound to the envelope protein domain III from dengue virus serotype 4 was used as a template to explore the molecular origins of the broader cross-reactivity and increased in vivo potency of mAb 513, compared to the parent murine mAb 4E11, using molecular dynamics simulations and network analyses. These two methods are a powerful complement to existing structural and binding data and detail specific interactions that underpin the differential binding of the two antibodies. We found that a Glu at position H55 (GluH55) from the second Complementarity Determining Region of the Heavy chain (CDR-H2) which corresponds to Ala in 4E11, is a major contributor to the enhancement in the interactions of mAb 513 compared to 4E11. Importantly, we also validate the importance of GluH55 using site-directed mutagenesis followed by isothermal titration calorimetry measurements.
Molecular recognition of RAS/RAF complex at the membrane: Role of RAF cysteine-rich domain
Authors: Travers Timothy , López Cesar A. , Van Que N. , Neale Chris , Tonelli Marco , Stephen Andrew G. , Gnanakaran S. 
Published: May 2018

AbstractActivation of RAF kinase involves the association of its RAS-binding domain (RBD) and cysteine-rich domain (CRD) with membrane-anchored RAS. However, the overall architecture of the RAS/RBD/CRD ternary complex and the orientations of its constituent domains at the membrane remain unclear. Here, we have combined all-atom and coarse-grained molecular dynamics (MD) simulations with experimental data to construct and validate a model of membrane-anchored CRD, and used this as a basis to explore models of membrane-anchored RAS/RBD/CRD complex. First, simulations of the CRD revealed that it anchors to the membrane via insertion of its two hydrophobic loops, which is consistent with our NMR measurements of CRD bound to nanodiscs. Simulations of the CRD in the context of membrane-anchored RAS/RBD then show how CRD association with either RAS or RBD could play an unexpected role in guiding the membrane orientations of RAS/RBD. This finding has implications for the formation of RAS-RAS dimers, as different membrane orientations of RAS expose distinct putative dimerization interfaces.
Linear Elastic Fracture Mechanics Characterization of an Anisotropic Shale
Authors: Luo Y. , Xie H. P. , Ren L. , Zhang R. , Li C. B. , Gao C. 
Published: May 2018

AbstractThe existence of bedding planes in natural shale formations makes the fracture characterization remarkably complicated. To achieve a further understanding of the anisotropic crack extension behaviors of shale using a linear elastic fracture mechanics approach, four groups of three-point bending tests on Longmaxi shale from southeast Chongqing were conducted in this study with different bedding plane inclination angles. The fracture propagation paths were observed using a scanning electron microscope. The results indicated that cracks initiated along the bedding plane when the bedding plane inclination angle (i.e., the angle between the loading direction and the normal direction of the bedding plane) was relatively large; in contrast, cracks penetrated into the matrix and induced higher fracture toughness in cases with lower bedding plane inclination angle. Brittle fractures occurred in the tested shale, and the fracture strength was strongly dependent on the bedding plane inclination angle. Meanwhile, the stress field around the crack tip was analyzed theoretically. The results indicated that the crack tip stress field of anisotropic shale is not only determined by the stress intensity factor but also related to the elastic constants and bedding plane inclination angle. Furthermore, a criterion for determining whether a crack extends along the bedding plane was developed by distinguishing the differences in the strengths of the shale bedding and the matrix.
Unravelling the origin of the basket stars and their allies (Echinodermata, Ophiuroidea, Euryalida)
Authors: Thuy Ben , Stöhr Sabine 
Published: May 2018

AbstractEuryalids, which include the spectacular basket stars, form a morphologically aberrant group of brittle stars. Surprisingly, the most recent molecular work found them to be sister to ophiurid brittle stars, thus challenging the traditional dichotomy between euryalids and non-euryalids, and leaving an enormous ghost lineage of more than 100 million years between the oldest unambiguous euryalid fossils and their predicted divergence from ophiurids during the Triassic. Here we examine both previously known and newly collected fossils to explore the evolutionary history of euryalids. A morphology-based phylogenetic estimate confirms the Triassic Aspiduriella as a basal member of the euryalid clade that superficially resembles members of the living ophiurid sister clades. Furthermore, we use lateral arm plates and vertebrae to identify two new Jurassic ophiuroids, Melusinaster alissawhitegluzae and Melusinaster arcusinimicus, as early euryalids that are morphologically intermediate between Aspiduriella and extant euryalids. Our phylogenetic analysis is the first to combine data from completely preserved skeletons and from microfossils in order to bridge morphological and stratigraphical gaps between the sampled taxa. It fills a major gap in the fossil record of euryalids and sets a robust phylogenetic framework to understand the morphological transition from ophiurid-like ancestors to the typical modern euryalids better.
Expression of hybrid fusion protein (Cry1Ac::ASAL) in transgenic rice plants imparts resistance against multiple insect pests
Authors: Boddupally Dayakar , Tamirisa Srinath , Gundra Sivakrishna Rao , Vudem Dashavantha Reddy , Khareedu Venkateswara Rao 
Published: May 2018

AbstractTo evolve rice varieties resistant to different groups of insect pests a fusion gene, comprising DI and DII domains of Bt Cry1Ac and carbohydrate binding domain of garlic lectin (ASAL), was constructed. Transgenic rice lines were generated and evaluated to assess the efficacy of Cry1Ac::ASAL fusion protein against three major pests, viz., yellow stem borer (YSB), leaf folder (LF) and brown planthopper (BPH). Molecular analyses of transgenic plants revealed stable integration and expression of the fusion gene. In planta insect bioassays on transgenics disclosed enhanced levels of resistance compared to the control plants. High insect mortality of YSB, LF and BPH was observed on transgenics compared to that of control plants. Furthermore, honeydew assays revealed significant decreases in the feeding ability of BPH on transgenic plants as compared to the controls. Ligand blot analysis, using BPH insects fed on cry1Ac::asal transgenic rice plants, revealed a modified receptor protein-binding pattern owing to its ability to bind to additional receptors in insects. The overall results authenticate that Cry1Ac::ASAL protein is endowed with remarkable entomotoxic effects against major lepidopteran and hemipteran insects. As such, the fusion gene appears promising and can be introduced into various other crops to control multiple insect pests.