bluuebunny/crossref_metadata_2025_split huggingface dataset:
iTRAQ‐based proteomic analysis of human umbilical vein endothelial cells with platelet endothelial aggregation receptor‐1 knockdown
Authors: Yue Yongjian , Liu Shengguo , Han Xuemei , Wang Minlian , Li Yazhen , Huang Qijun , Li Bo , Yang Mo , Dai Yong , Fu Yingyun 
Published: February 2019

AbstractThe disorders of hemostasis and coagulation were believed to be the main contributors to the pathogenesis of pulmonary thromboembolism (PTE), and platelets are the basic factors regulating hemostasis and coagulation and play important roles in the process of thrombosis. This study investigated the proteome of human umbilical vein endothelial cells (HUVECs) with platelet endothelial aggregation receptor‐1 (PEAR1) knockdown using the isobaric tags for relative and absolute quantitation (iTRAQ) method and analyzed the role of differential abundance proteins (DAPs) in the regulation of platelets aggregation. Our results showed that the conditioned media‐culturing HUVECs with PEAR1 knockdown partially suppressed the adenosine diphosphate (ADP)‐induced platelet aggregation. The proteomics analysis was performed by using the iTRAQ technique, and a total of 215 DAPs (124 protein was upregulated and 91 protein were downregulated) were identified. The Gene Ontology (GO) enrichment analysis showed that proteins related to platelet α granule, adenosine triphosphate metabolic process, and endocytosis were significantly enriched. Further, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also identified the significant enrichment of endocytosis‐related pathways. The real‐time polymerase chain reaction assay confirmed that the expression of P2Y12, mitochondrial carrier 2, NADH dehydrogenase (ubiquinone) iron‐sulfur protein 3, and ubiquinol‐cytochrome c reductase hinge protein are significantly downregulated in the HUVECs with PEAR1 knockdown. In conclusion, our in vitro results implicated that DAPs induced by PEAR1 knockdown might contribute to the platelet aggregation. Proteomic studies by employing GO enrichment and KEGG pathway analysis suggested that the potential effects of DAPs on platelet aggregation may be linked to the balance of ADP synthesis or degradation in mitochondria.
Church support networks of African Americans: The impact of gender and religious involvement
Authors: Nguyen Ann W. , Taylor Robert Joseph , Chatters Linda M. , Hope Meredith O. 
Published: February 2019

AbstractWe examined the sociodemographic and religious involvement correlates of church support networks in a nationally representative sample of African Americans across the adult life span. Data from the National Survey of American Life was used for analysis. Ordinary least squares regression was conducted to identify correlates of frequency of contact, subjective closeness, provision and receipt of overall support, receipt of emotional support, and negative interactions with church members. We also investigated differences in church support networks separately for men and women. Religious involvement was positively associated with church support network indicators (i.e., frequency of contact). Church support network indicators also varied by age, gender, education, family income, marital status, and region. The findings indicate that for many African Americans, church members are an integral component of their support networks and underscore the importance of social integration in church networks for social support exchanges. Moreover, these church support network characteristics are patterned by sociodemographic characteristics.
The influences of neighborhood disorder on early childhood externalizing problems: The roles of parental stress and child physical maltreatment
Authors: Pei Fei , Wang Xiafei , Yoon Susan , Tebben Erin 
Published: February 2019

AbstractAimsThe current study examined both the direct and indirect influences of neighborhood disorder on early childhood externalizing problems among 3,036 three‐year‐old children.Methods and ResultsData used in this study were drawn from the Fragile Families and Child Wellbeing Study (N = 3,036). The results of Structural Equation Modeling (SEM) indicated that living in disordered neighborhoods is directly associated with higher levels of early childhood externalizing problems. Moreover, parental stress and physical maltreatment significantly mediated the relationship between neighborhood disorder and externalizing problems at children's age 3.ConclusionParents living in chaotic neighborhoods may accumulate higher levels of parental stress and physically maltreat their children, which in turn increased their children's externalizing problems. Our findings support the importance of interventions of early childhood externalizing problems at both the micro and macro levels. Intervention programs that address neighborhood disorders, as well as challenges in parenting, are recommended.
RETRACTED: microRNA‐211 promotes invasion and migration of colorectal cancer cells by targeting FABP4 via PPARγ
Authors: Zhao Dongmei , Ma Yanying , Li Xu , Lu Xiaoyu 
Published: February 2019

AbstractFatty acid binding protein 4 (FABP4) is a novel tumor regulator that is abnormally expressed in many human cancers. In our study, upregulated microRNA‐211 (miR‐211) and reduced FABP4 expression were detected in colorectal cancer (CRC) patients and CRC cells. Mimic miR‐211 or anti‐miR‐211 were transfected to investigate the effects of miR‐211 on SW480 cells. The results showed that miR‐211 promoted but anti‐miR‐211 inhibited cell migration, invasion, and epithelial–mesenchymal transition (EMT) of SW480 cells. Luciferase activity was decreased after cotransfection with miR‐211 and WT‐FABP4‐UTR in SW480 cells. And reduced FABP4 protein expression by miR‐211 indicated that FABP4 was the targeted gene of miR‐211. miR‐211 inhibited the activation of peroxisome proliferator‐activated receptor (PPAR) γ, whereas overexpression of FABP4 reversed that effect. Finally, FABP4 inhibited the migration, invasion, and EMT of SW480 cells, whereas PPARγ agonist reversed the effects of FABP4. Thus, the miR‐211/FABP4/PPARγ axis may be a novel target for CRC therapy.
RAS/MAPK signaling functions in oxidative stress, DNA damage response and cancer progression
Authors: Rezatabar Setareh , Karimian Ansar , Rameshknia Vahid , Parsian Hadi , Majidinia Maryam , Kopi Tayebeh Azramezani , Bishayee Anupam , Sadeghinia Ali , Yousefi Mehdi , Monirialamdari Mohsen , Yousefi Bahman 
Published: February 2019

AbstractMitogen‐activated protein kinase (MAPK) signaling pathways organize a great constitution network that regulates several physiological processes, like cell growth, differentiation, and apoptotic cell death. Due to the crucial importance of this signaling pathway, dysregulation of the MAPK signaling cascades is involved in the pathogenesis of various human cancer types. Oxidative stress and DNA damage are two important factors which in common lead to carcinogenesis through dysregulation of this signaling pathway. Reactive oxygen species (ROS) are a common subproduct of oxidative energy metabolism and are considered to be a significant physiological modulator of several intracellular signaling pathways including the MAPK pathway. Studies demonstrated that the MAP kinases extracellular signal‐regulated kinase (ERK) 1/2 and p38 were activated in response to oxidative stress. In addition, DNA damage is a partly common circumstance in cell life and may result in mutation, cancer, and even cell death. Recently, accumulating evidence illustrated that the MEK/ERK pathway is associated with the suitable performance of cellular DNA damage response (DDR), the main pathway of tumor suppression. During DDR, the MEK/ERK pathway is regularly activated, which contributes to the appropriate activation of DDR checkpoints to inhibit cell division. Therefore, the aim of this review is to comprehensively discuss the critical function of MAPK signaling in oxidative stress, DNA damage, and cancer progression.
Effects of AURKA‐mediated degradation of SOD2 on mitochondrial dysfunction and cartilage homeostasis in osteoarthritis
Authors: Yang Cheng , You Di , Huang Jun , Yang Bo , Huang Xianzhe , Ni Jiangdong 
Published: February 2019

AbstractThis study aimed to investigate the mechanism of the ubiquitinase Aurora kinase A (AURKA) in the occurrence of osteoarthritis (OA) by mediating mitochondrial stress. Bioinformatic predictions revealed 2247 differentially expressed genes (DEGs) in the normal and OA tissues. According to the UbiNet database, 39 DEGs that code for ubiquitination enzymes was screened. AURKA was highly expressed in OA tissues and cells. AURKA interference inhibited the elevation of matrix metalloproteinase‐13 (MMP‐13). (MMP13), sex determining region Y‐box 9 (Sox9), and a disintegrin and metalloproteinase with thrombospondin motifs‐5 (ADAMTS5) expression and the reduction of collagen type IIα (Col2a1) and Aggrecan expression in interleukin‐1 β (IL‐1β) induced chondrocytes. The animal experiments proved that depleting AURKA could repress the occurrence of OA. Superoxide dismutase 2 (SOD2) was determined to be AURKA ubiquitination substrate via AURKA expression and bioinformatic prediction experiments. SOD2 expression was lower in OA tissues, but higher in normal joint tissues. AURKA interference activates SOD2. Meanwhile, the IP results confirmed that AURKA could bind to SOD2 and degrade it through K48 ubiquitination. Modification and overexpression of AURKA reduce SOD2 levels. AURKA interference can reverse the reactive oxygen species elevation caused by SOD2 overexpression or lysine‐48 (K48) mutation, respectively, leading to mitochondrial dysfunction. Furthermore, AURKA silencing suppressed the occurrence of OA induced by mitochondrial activation. These findings suggest that ubiquitination of AURKA lowers SOD2 expression and affects mitochondrial dysfunction to repress the occurrence of OA. The results of the current study reveal that AURKA ubiquitination influences mitochondrial dysfunction and suppresses the occurrence of OA via degradation of SOD2. These data reveal novel potential targets for OA treatment
Separation of a ternary mixture with multiple azeotropes via pressure‐swing distillation
Authors: Wang Chao , Guang Chao , Cui Yue , Zhang Zhishan , Zhang Xiaokang 
Published: February 2019

AbstractBACKGROUNDThe ternary mixture of tetrahydrofuran (THF), ethanol and water contains multiple azeotropes with the sensitivity to pressure variation. The pressure‐swing distillation (PSD) is a promising method to achieve the system's separation objective since it avoids many potential problems caused by adding a third component.RESULTA variety of separation sequences were synthesized on the ternary diagram, including the sequences of WLEMTH, WLTH EM, THEMWL, THWLEM, EMTHWL and EMWLTH. The optimal design parameters of these sequences were obtained based on the minimum total annual cost (TAC) by using the sequential iterative global optimization algorithm. Furthermore, six kinds of heat integration that improve energy efficiency were implemented for the optimal alternative of EMTHWL.CONCLUSIONThe results show that the EMTHWL configuration has the lowest TAC, saving 37.84% of the economic cost compared to the THEMWL which has the highest TAC. The partial heat integration among three columns can achieve almost the same reductions in energy requirements and carbon dioxide emissions (44.9%) as the full heat integration among three columns (45.4%). In addition, it is predictable that the partially heat‐integrated alternative compared to other fully heat‐integrated schemes has less undesirable side effects in the aspect of dynamic control. © 2019 Society of Chemical Industry
Validation of International Club of Ascites subclassification of stage 1 acute kidney injury in chronic liver disease
Authors: Khatua Chitta R , Sahu Saroj K , Barik Rakesh K , Pradhan Subhasis , Panigrahi Subhendu , Mishra Debakanta , Singh Shivaram P 
Published: February 2019

Background and AimAcute kidney injury (AKI) commonly occurs in patients with chronic liver disease (CLD). As per the International Club of Ascites, AKI is classified into three stages; stage 1 has recently been divided into subgroups 1A and 1B. We performed a prospective study to validate the association between subgrouping and outcome.MethodsThis study was conducted using decompensated cirrhosis (DC) patients hospitalized in the Gastroenterology ward between August 2016 and May 2018. Demographic, clinical, and laboratory parameters were compared between AKI 1A and AKI 1B patients. The duration of hospitalization and outcome were compared.ResultsA total of 528 subjects were enrolled; 296 (56.1%) had AKI, and of them, 61.48% (n = 182) had stage 1, 20.95% (n = 62) had stage 2, and 17.57% (n = 52) had stage 3 AKI. Of the enrolled patients, 100 (54.94%) had early (AKI 1A) and 82 (45.06%) had late stage 1 AKI (AKI 1B). Patients with AKI 1B had higher total leucocyte count, total bilirubin, serum urea, serum creatinine (SCr), model for end‐stage liver disease (MELD), MELD‐Na+, and child‐turcotte‐pugh (CTP) score and decreased serum albumin than AKI 1A. The prevalence of hepatorenal syndrome (HRS), acute on chronic liver failure (ACLF) were higher in AKI 1B patients, and they had a prolonged hospital stay compared to AKI 1A patients. Furthermore, AKI 1B patients had significantly lower survival both at 28 days and 90 days.ConclusionOur study validates the subclassification of stage 1 AKI. Patients with AKI 1B more often progress to higher AKI stages with significantly lower 28‐day and 90‐day survival rates. Results justify subclassification and suggest the need for early intervention. The small increase in SCr should be viewed with caution in AKI stage 1A.
Long non‐coding RNA TTN‐AS1 facilitates tumorigenesis of papillary thyroid cancer through modulating the miR‐153‐3p/ZNRF2 axis
Authors: Cui Zhenghui , Luo Zhiyan , Lin Zimei , Shi Liuhong , Hong Yurong , Yan Caoxin 
Published: February 2019

AbstractBackgroundLong non‐coding RNAs (lncRNAs) are crucial modulators in the tumorigenesis of numerous cancers, including papillary thyroid cancer (PTC). However, it is unclear whether lncRNA TTN antisense RNA 1 (TTN‐AS1) can regulate PTC progression. The present study aimed to reveal the mechanism and function of TTN‐AS1 in PTC.MethodsTTN‐AS1 expression in 92 pairs PTC tissues and four PTC cells was measured via a quantitative reverse transcriptase‐polymerase chain reaction assay. The relationship of TTN‐AS1 expression and clinical pathological features of PTC patients was analyzed using a chi‐squared test. The biofunction of TTN‐AS1 in PTC was identified by loss or gain‐of‐function assays. Based on bioinformatics analysis and mechanism experiments, the molecular mechanism of TTN‐AS1 was analyzed and identified.ResultsA high level of TTN‐AS1 was observed in PTC tissues and cells. The expression level of TTN‐AS1 is possibly associated with lymphatic metastasis, TNM stage and the overall survival of PTC patients. Functionally, TTN‐AS1 knockdown inhibited cell proliferation, migration, invasion and epithelial–mesenchymal transition in PTC, whereas overexpression of TTN‐AS1 led to the opposite results. Mechanistically, TTN‐AS1 acted as a competing endogenous RNA by sponging microRNA‐153‐3p (miR‐153‐3p) to elevate zinc and ring finger 2 (ZNRF2) expression. Additionally, a high level of TTN‐AS1 in PTC was closely correlated with the activity of the phosphoinositide 3‐kinase (PI3K)/Akt/mechanistic target of rapamycin (mTOR) pathway.ConclusionsThe findings obtained in the present study indicate that TTN‐AS1 facilitated PTC progression by regulating the miR‐153‐3p/ZNRF2 axis and activating the PI3K/Akt/mTOR pathway.
Novel Furochromone Derivatives: Synthesis and Anticancer Activity Studies
Authors: Demir Senem , Özen Cigdem , Ceylan‐Ünlüsoy Meltem , Öztürk Mehmet , Bozdağ‐Dündar Oya 
Published: February 2019

Medicinal plant extracts have been used for medical purposes throughout human history. In this study, khellin, having furochromone structure, which is obtained from a well‐known traditional medicinal plant, was selected. A series of furochromonyl compounds (K1–K14) were synthesized for their anticancer activities. Furochromonyl compounds (K1–K14) were synthesized by Knoevenagel reaction of substituted 2,4‐thiazolidinediones (Ia–j)/rhodanines (Ik–n) with khellin‐2‐carboxaldehyde (V), and their cytotoxicity was investigated in 22 cancer cell lines, which were originated from tissues such as the liver, breast, colon, and cervix. As the first step, two hepatocellular carcinoma cell lines Huh7 and PLC/PRF/5 (Alexander cells) were treated with 10 μM of each compound for 72 h, and then sulforhodamine B assay was performed to analyze their anti‐growth activities. Ethyl 2‐(5‐((4,9‐dimethoxy‐5‐oxo‐5H‐furo[3,2‐g]chromen‐7‐yl)methylene)‐4‐oxo‐2‐thioxothiazolidin‐3‐yl)acetate (K11) was found as the most cytotoxic compound of primary screening. Afterwards, 12 hepatocellular carcinoma, seven breast cancer, two colon cancer, and a cervical cancer cell lines were selected to test K11 for 72 h at multiple concentrations to determine 50% effective doses. Results showed that the 14 cell lines were affected by K11 quantities lower than 10 μM. The structure of K11, which is particularly effective on breast cancers, can be used to slow down the progression of tumors. Furthermore, the discovery of more effective compounds can be carried out on the basis of this structure.
Comparative limb bone scaling in turtles: Phylogenetic transitions with changes in functional demands?
Authors: Young Vanessa K Hilliard , Baeza Juan Antonio , Blob Richard W. 
Published: February 2019

AbstractSeveral terrestrial vertebrate clades include lineages that have evolved nearly exclusive use of aquatic habitats. In many cases, such transitions are associated with the evolution of flattened limbs that are used to swim via dorsoventral flapping. Such changes in shape may have been facilitated by changes in limb bone loading in novel aquatic environments. Studies on limb bone loading in turtles found that torsion is high relative to bending loads on land, but reduced compared to bending during aquatic rowing. Release from torsion among rowers could have facilitated the evolution of hydrodynamically advantageous flattened limbs among aquatic species. Because rowing is regarded as an intermediate locomotor stage between walking and flapping, rowing species might show limb bone flattening intermediate between the tubular shapes of walkers and the flattened shapes of flappers. We collected measurements of humeri and femora from specimens representing four functionally divergent turtle clades: sea turtles (marine flappers), softshells (specialized freshwater rowers), emydids (generalist semiaquatic rowers), and tortoises (terrestrial walkers). Patterns of limb bone scaling with size were compared across lineages using phylogenetic comparative methods. Although rowing taxa did not show the intermediate scaling patterns we predicted, our data provide other functional insights. For example, flattening of sea turtle humeri was associated with positive allometry (relative to body mass) for the limb bone diameter perpendicular to the flexion‐extension plane of the elbow. Moreover, softshell limb bones exhibit positive allometry of femoral diameters relative to body mass, potentially helping them maintain their typical benthic position in water by providing additional weight to compensate for shell reduction. Tortoise limb bones showed positive allometry of diameters, as well as long humeri, relative to body mass, potentially reflecting specializations for resisting loads associated with digging. Overall, scaling patterns of many turtle lineages appear to correlate with distinctive behaviors or locomotor habits.
A 10‐mW 3.9‐dB NF transformer‐based V‐band low‐noise amplifier in 65‐nm CMOS
Authors: Yu Yiming , Wu Yunqiu , Zhao Chenxi , Liu Huihua , Ban Yongling , Kang Kai 
Published: February 2019

AbstractA V‐band low‐noise amplifier (LNA) employing a Gm‐boosting technique is presented in this paper. With the transformers, which are applied between the adjacent stages, the transconductances of the following transistors are boosted. Thus, the gain of the circuit is effectively enhanced. The noise figure (NF) is also decreased with the technique. Utilizing a commercial 65‐nm CMOS technology, the LNA is demonstrated. The measurement results show that the LNA achieves a maximum gain of 17.4 dB at 57.1 GHz with 10.9‐GHz 3‐dB gain bandwidth. The measured NF of the LNA is from 3.95 dB to 4.6 dB at 53 to 64 GHz. The tested input 1‐dB gain compression point (IP1dB) is −14.2 dBm at 57 GHz. The DC power consumption is only 10 mW with 1‐V power supply. The chip area is only 0.255 mm2 with all testing pads.
Microstrip transmission line model‐fitting approach for characterization of textile materials as dielectrics and conductors for wearable electronics
Authors: Le Duc , Kuang Ye , Ukkonen Leena , Björninen Toni 
Published: February 2019

AbstractCharacterization of unconventional materials is essential in the field of microwave engineering. In this study, we present a method for the microwave characterization of conductive and nonconductive textile materials. Our approach is based on fitting the material parameters of a numerical microstrip transmission line model so that the simulated signal transmission properties match with the data we measure from implemented test lines. Unlike many conventional test structures, such as cavity resonators and waveguides, microstrip lines can be readily manufactured in textile technology with rather relaxed tolerances in the fabrication process. In addition, our method provides estimates for all three key material parameters: frequency‐dependent relative permittivity and loss tangent of the nonconductive line substrate and the bulk conductivity of the strip conductor. For validation, we have characterized conventional microwave laminates and compared the data with the datasheets and literature and used our parameter estimates for the textile materials to optimize fully textile‐based low‐pass filters. Overall, the results confirm the applicability of our method for microwave engineering with textile materials.
Automatic analysis of cross‐sectional cerebral asymmetry on 3D in vivo MRI scans of human and chimpanzee
Authors: Xiang Li , Crow Timothy , Roberts Neil 
Published: February 2019

AbstractOne prominent feature of human brain asymmetry is the cerebral torque. To investigate whether this characteristic is shared with chimpanzees who are our closest extant relative, we developed an automatic method to compute cerebral hemisphere width and perimeter length on consecutive 2D sections through 3D MR images obtained in vivo for 91 human and 78 chimpanzee brains. In brief, contiguous inter‐hemispheric width and perimeter asymmetries were calculated on coronal sections, which in profile allow us to examine asymmetry in relation to speciation. The right frontal and left occipital asymmetry (greater posteriorly in females) distinguishes humans from chimpanzees. This result is consistent with a major saltational (discontinuity) event occurring at some point after the separation of humanity and the great apes in the last 6 million years.