bluuebunny/crossref_metadata_2025_split huggingface dataset:
Processing traits and digestibility of extruded dog foods with soy protein concentrate
Authors: Venturini K. S. , Sarcinelli M. F. , Baller M. A. , Putarov T. C. , Malheiros E. B. , Carciofi A. C. 
Published: April 2018

SummarySoya bean protein concentrate (SPC) with two particle sizes were evaluated on extrusion parameters, kibble formation, digestibility and palatability of dog foods. Eight diets were extruded:PBM—control diet based on poultry by‐product meal (PBM);GM—a diet in which corn gluten meal (GM) replaced 45% of the diet protein;cSPC15%,cSPC30% andcSPC45%—diets in whichSPCof coarse particle size (600 μm) replaced 15%, 30% and 45% of the diet protein; andsSPC15%,sSPC30% andsSPC45%—diets in whichSPCof small particle size (200 μm) replaced 15%, 30% and 45% of the diet protein. The digestibility of nutrients was evaluated for thePBM,GM,cSPC45% andsSPC45% diets, using six dogs per food. ThePBM,GMandcSPC45% diets were compared for palatability. Data were submitted for analysis of variance, and the means were compared by polynomial contrasts or Tukey's test (p < .05). ThecSPCincreased the specific mechanical energy (SME) application, extrusion temperature and pressure linearly, resulting in lower kibble density and higher expansion and starch gelatinization (SG) (p < .01). When comparing thePBM,GM,cSPC45% andsSPC45% diets, higherSME, extrusion temperature and pressure,SGand kibble expansion were verified for thecSPC45% diet (p < .05). TheDM, fat and crude protein digestibility were similar among diets. FaecalpH, ammonia and lactate did not differ, demonstrating that the removal of oligosaccharides and soluble non‐starch polysaccharides ofSPCproduces an ingredient with mostly non‐fermentable fibre. Dogs preferred thePBMto theGMdiet (p < .05), but consumed thePBMandcSPC45% foods equally. In conclusion,SPCexhibited good extrusion functionality, favouring kibble expansion andSG, with high digestibility, similar to that ofPBM. The removal of soluble compounds from soya beans resulted in an ingredient with low fermentable fibre content, which did not alter faecal formation or characteristics.
An unusual case of anemia in a cardiac transplant recipient
Authors: Lehto Elizabeth , Raj Ashok , Sparks Joshua 
Published: April 2018

AbstractAnemia is a well‐described comorbidity in patients with heart failure and has been associated with decreased survival rates after heart transplant. The causes of anemia are broad, and identification of the underlying etiology is critical for management. Herein, we report an unusual case of severe anemia complicating cardiac transplantation.
Consensus minimum data set for lung cancer multidisciplinary teams: Results of a Delphi process
Authors: Stone Emily , Rankin Nicole , Phillips Jane , Fong Kwun , Currow David C. , Miller Alistair , Largey Geraldine , Zielinski Robert , Flynn Peter , Shaw Tim 
Published: April 2018

AbstractBackground and objectiveWhile multidisciplinary team (MDT) care in lung cancer is widely practiced, there are few guidelines for MDT on best data collection strategies. MDT meetings need ready access to information for the provision of optimal treatment recommendations (the primary purpose of the meeting), audit of team performance and benchmarking. This study aimed to develop a practical data set designed for these goals through a recognized consensus process with health professionals who participate in formal MDT settings.MethodsA modified Delphi process with three iterations (two surveys and one consensus conference) was carried out involving over 100 Australian lung cancer MDT health professionals.ResultsIn total, 122 lung cancer MDT health professionals responded to the Round 1 survey from over 350 invitees. Of the 122, 98 were available for invitation to Round 2. Of 98, 52 (53%) invitees responded to the Round 2 survey. After two rounds, 51 data elements across 8 domains (patient demographics, risk factors, biopsy data, staging, timeliness, treatment, follow‐up and patient selection) achieved consensus, defined as 80% agreement. For Round 3, 33 MDT lead clinicians were invited to participate in a consensus conference. Of 33, 14 (42%) invitees distilled the 47 data elements into 23 elements across 8 domains to address the study objectives.ConclusionA practical data set for lung cancer MDT to use for optimal treatment recommendations and to evaluate team performance was developed through recognized consensus methodology. Access to streamlined, relevant and feasible data collection strategies may improve MDT decision‐making, audit of team performance and facilitate benchmarking.
The Effect of Geographic Proximity to Unconventional Oil and Gas Development on Public Support for Hydraulic Fracturing
Authors: Boudet Hilary S. , Zanocco Chad M. , Howe Peter D. , Clarke Christopher E. 
Published: April 2018

AbstractWith the rapid growth of unconventional oil and natural gas development transforming the U.S. economic and physical landscape, social scientists have increasingly explored the spatial dynamics of public support for this issue—that is, whether people closer to unconventional oil and gas development are more supportive or more opposed. While theoretical frameworks like construal‐level theory and the “Not in My Backyard” (or NIMBY) moniker provide insight into these spatial dynamics, case studies in specific locations experiencing energy development reveal substantial variation in community responses. Larger‐scale studies exploring the link between proximity and support have been hampered by data quality and availability. We draw on a unique data set that includes geo‐coded data from national surveys (nine waves; n = 19,098) and high‐resolution well location data to explore the relationship between proximity and both familiarity with and support for hydraulic fracturing. We use two different measures of proximity—respondent distance to the nearest well and the density of wells within a certain radius of the respondent's location. We find that both types of proximity to new development are linked to more familiarity with hydraulic fracturing, even after controlling for various individual and contextual factors, but only distance‐based proximity is linked to more support for the practice. When significant, these relationships are similar to or exceed the effects of race, income, gender, and age. We discuss the implications of these findings for effective risk communication as well as the importance of incorporating spatial analysis into public opinion research on perceptions of energy development.
Enhancing data privacy with semantic trajectories: A raster‐based framework for GPS stop/move management
Authors: Wang Yang , McArthur David 
Published: April 2018

AbstractTracking facilities on smartphones generate enormous amounts of GPS trajectories, which provide new opportunities to study movement patterns and improve transportation planning. Converting GPS trajectories into semantically meaningful trips is attracting increasing research effort with respect to the development of algorithms, frameworks, and software tools. There are, however, few works focused on designing new semantic enrichment functionalities taking privacy into account. This article presents a raster‐based framework which not only detects significant stop locations, segments GPS records into stop/move structures, and brings semantic insights to trips, but also provides possibilities to anonymize users’ movements and sensitive stay/move locations into raster cells/regions so that a multi‐level data sharing structure is achieved for a variety of data sharing purposes.
Reciprocal Effects of Antiretroviral Drugs Used To Treat HIV Infection on the Fibroblast Growth Factor 21/β-Klotho System
Authors: Moure Ricardo , Domingo Pere , Villarroya Joan , Gasa Laura , Gallego-Escuredo José M. , Quesada-López Tania , Morón-Ros Samantha , Maroto Alberto F. , Mateo Gracia M. , Domingo Joan C. , Villarroya Francesc , Giralt Marta 
Published: April 2018

ABSTRACTFollowing antiretroviral therapy, HIV-infected patients show increased circulating levels of the antidiabetic hormone fibroblast growth factor 21 (FGF21). In contrast, the expression of the FGF21-obligatory coreceptor β-Klotho (KLB) is reduced in target tissues. This situation is comparable to the FGF21 resistance status observed in obesity and type 2 diabetes. Here, we performed the first systematic study of the effects of distinct members of different antiretroviral drug classes on the FGF21/KLB system in human hepatic, adipose, and skeletal muscle cells. Most protease inhibitors and the nonnucleoside reverse transcriptase inhibitor efavirenz induced FGF21 gene expression. Neither nucleoside reverse transcriptase inhibitors nor the viral entry inhibitor maraviroc had any effect. Among the integrase inhibitors, elvitegravir significantly induced FGF21 expression, whereas raltegravir had minor effects only in adipose cells. In human hepatocytes and adipocytes, known target cells of FGF21 action, efavirenz, elvitegravir, and the lopinavir-ritonavir combination exerted inhibitory effects on KLB gene expression. Drug treatments that elicited FGF21 induction/KLB repression were those found to induce endoplasmic reticulum (ER) stress and oxidative stress. Notably, the pharmacological agents thapsigargin and tunicamycin, which induce these stress pathways, mimicked the effects of drug treatments. Moreover, pharmacological inhibitors of either ER or oxidative stress significantly impaired lopinavir–ritonavir-induced regulation of FGF21, but not KLB. In conclusion, the presentin vitroscreen study identifies the antiretroviral drugs that affect FGF21/KLB expression in human cells. The present results could have important implications for the management of comorbidities resulting from side effects of specific antiretroviral drugs for the treatment of HIV-infected patients.
Genome Sequence of a Multidrug-Resistant Candida haemulonii Isolate from a Patient with Chronic Leg Ulcers in Israel
Authors: Chow Nancy A. , Gade Lalitha , Batra Dhwani , Rowe Lori A. , Juieng Phalasy , Ben-Ami Ronen , Loparev Vladimir N. , Litvintseva Anastasia P. 
Published: April 2018

ABSTRACT

Candida haemulonii
is an emerging multidrug-resistant yeast that can cause invasive candidiasis. Here, we report the first genome sequence of
C. haemulonii
(isolate B11899) generated using PacBio sequencing technology. The estimated genome size was 13.3 Mb, with a GC content of 45.19%.
Draft Genome Sequence of Aeromonas cavernicola sp. nov. DSM 24474
T
, Isolated from a Cavern Brook in the Moravia Region of the Czech Republic
Authors: Colston S. M. , Navarro A. , Martinez-Murcia A. J. , Graf J. 
Published: April 2018

ABSTRACT

Species of the
Aeromonas
genus can be found in numerous environmental milieus, including various water sources, and some species cause disease in animals. We present here the draft genome sequence for
Aeromonas cavernicola
DSM 24474
T
, a novel species isolated from a freshwater brook within a cavern in the Czech Republic.
Draft Genome Sequences of Four Strains of Recently Established Novel Veillonella Species Isolated from Human Oral Cavities
Authors: Mashima Izumi , Liao Yu-Chieh , Sabharwal Amarpreet , Haase Elaine M. , Nakazawa Futoshi , Scannapieco Frank A. 
Published: April 2018

ABSTRACT

Veillonella
species are known to contribute to the formation of early oral biofilms and tend to be prevalent in people with poor oral hygiene status. Here, we report the draft genome sequences of 4 oral
Veillonella
strains that were established recently as novel species.
Draft Genome Sequences of New Genomospecies “
Candidatus
Pectobacterium maceratum” Strains, Which Cause Soft Rot in Plants
Authors: Shirshikov Fedor V. , Korzhenkov Aleksei A. , Miroshnikov Kirill K. , Kabanova Anastasia P. , Barannik Alla P. , Ignatov Alexander N. , Miroshnikov Konstantin A. 
Published: April 2018

ABSTRACT

Investigation of collections of phytopathogenic bacteria has revealed some strains distinct from known
Pectobacterium
spp. We report here the draft genome sequences of five such strains, isolated during the period of 1947 to 2012. Based on comparative genomics, we propose a new candidate genomospecies of the genus
Pectobacterium
, “
Candidatus
Pectobacterium maceratum.”
Draft Genome Sequences of 12 Clinical and Environmental Methicillin-Resistant Staphylococcus pseudintermedius Strains Isolated from a Veterinary Teaching Hospital in Washington State
Authors: Shah Devendra H. , Jones Lisa P. , Paul Narayan , Davis Margaret A. 
Published: April 2018

ABSTRACT

Methicillin-resistant
Staphylococcus pseudintermedius
(MRSP) is a globally emergent multidrug-resistant pathogen of dogs associated with nosocomial transmission in dogs and with potential zoonotic impacts. Here, we report the draft whole-genome sequences of 12 hospital-associated MRSP strains and their resistance genotypes and phenotypes.
The caudal fastigial nucleus and the steering of saccades toward a moving visual target
Authors: Bourrelly Clara , Quinet Julie , Goffart Laurent 
Published: April 2018

The caudal fastigial nuclei (cFN) are the output nuclei by which the medio-posterior cerebellum influences the production of visual saccades. We investigated in two head-restrained monkeys their contribution to the generation of interceptive saccades toward a target moving centrifugally by analyzing the consequences of a unilateral inactivation (10 injection sessions). We describe here the effects on saccades made toward a centrifugal target that moved along the horizontal meridian with a constant (10, 20, or 40°/s), increasing (from 0 to 40°/s over 600 ms), or decreasing (from 40 to 0°/s over 600 ms) speed. After muscimol injection, the monkeys were unable to foveate the current location of the moving target. The horizontal amplitude of interceptive saccades was reduced during contralesional target motions and hypermetric during ipsilesional ones. For both contralesional and ipsilesional saccades, the magnitude of dysmetria increased with target speed. However, the use of accelerating and decelerating targets revealed that the dependence of dysmetria upon target velocity was not due to the current velocity but to the required amplitude of saccade. We discuss these results in the framework of two hypotheses, the so-called “dual drive” and “bilateral” hypotheses. NEW & NOTEWORTHY Unilateral inactivation of the caudal fastigial nucleus impairs the accuracy of saccades toward a moving target. Like saccades toward a static target, interceptive saccades are hypometric when directed toward the contralesional side and hypermetric when they are ipsilesional. The dysmetria depends on target velocity, but the use of accelerating or decelerating targets reveals that velocity is not the crucial parameter. We extend the bilateral fastigial control of saccades and fixation to the production of interceptive saccades.
Functional roles of Kv1-mediated currents in genetically identified subtypes of pyramidal neurons in layer 5 of mouse somatosensory cortex
Authors: Guan Dongxu , Pathak Dhruba , Foehring Robert C. 
Published: April 2018

We used voltage-clamp recordings from somatic outside-out macropatches to determine the amplitude and biophysical properties of putative Kv1-mediated currents in layer 5 pyramidal neurons (PNs) from mice expressing EGFP under the control of promoters for etv1 or glt. We then used whole cell current-clamp recordings and Kv1-specific peptide blockers to test the hypothesis that Kv1 channels differentially regulate action potential (AP) voltage threshold, repolarization rate, and width as well as rheobase and repetitive firing in these two PN types. We found that Kv1-mediated currents make up a similar percentage of whole cell K+ current in both cell types, and only minor biophysical differences were observed between PN types or between currents sensitive to different Kv1 blockers. Putative Kv1 currents contributed to AP voltage threshold in both PN types, but AP width and rate of repolarization were only affected in etv1 PNs. Kv1 currents regulate rheobase, delay to the first AP, and firing rate similarly in both cell types, but the frequency-current slope was much more sensitive to Kv1 block in etv1 PNs. In both cell types, Kv1 block shifted the current required to elicit an onset doublet of action potentials to lower currents. Spike frequency adaptation was also affected differently by Kv1 block in the two PN types. Thus, despite similar expression levels and minimal differences in biophysical properties, Kv1 channels differentially regulate APs and repetitive firing in etv1 and glt PNs. This may reflect differences in subcellular localization of channel subtypes or differences in the other K+ channels expressed. NEW & NOTEWORTHY In two types of genetically identified layer 5 pyramidal neurons, α-dendrotoxin blocked approximately all of the putative Kv1 current (on average). We used outside-out macropatches and whole cell recordings at 33°C to show that despite similar expression levels and minimal differences in biophysical properties, Kv1 channels differentially regulate action potentials and repetitive firing in etv1 and glt pyramidal neurons. This may reflect differences in subcellular localization of channel subtypes or differences in the other K+ channels expressed.
A Survey on Data Reproducibility and the Effect of Publication Process on the Ethical Reporting of Laboratory Research
Authors: Boulbes Delphine R. , Costello Tracy , Baggerly Keith , Fan Fan , Wang Rui , Bhattacharya Rajat , Ye Xiangcang , Ellis Lee M. 
Published: April 2018

Abstract
Purpose: The successful translation of laboratory research into effective therapies is dependent upon the validity of peer-reviewed publications. However, several publications in recent years suggested that published scientific findings could be reproduced only 11% to 45% of the time. Multiple surveys attempted to elucidate the fundamental causes of data irreproducibility and underscored potential solutions, more robust experimental designs, better statistics, and better mentorship. However, no prior survey has addressed the role of the review and publication process on honest reporting.
Experimental Design: We developed an anonymous online survey intended for trainees involved in bench research. The survey included questions related to mentoring/career development, research practice, integrity, and transparency, and how the pressure to publish and the publication process itself influence their reporting practices.
Results: Responses to questions related to mentoring and training practices were largely positive, although an average of approximately 25% did not seem to receive optimal mentoring. A total of 39.2% revealed having been pressured by a principle investigator or collaborator to produce “positive” data. About 62.8% admitted that the pressure to publish influences the way they report data. The majority of respondents did not believe that extensive revisions significantly improved the manuscript while adding to the cost and time invested.
Conclusions: This survey indicates that trainees believe that the pressure to publish affects honest reporting, mostly emanating from our system of rewards and advancement. The publication process itself affects faculty and trainees and appears to influence a shift in their ethics from honest reporting (“negative data”) to selective reporting, data falsification, or even fabrication. Clin Cancer Res; 24(14); 3447–55. ©2018 AACR.
Involvement of Prokineticin 2–expressing Neutrophil Infiltration in 5-Fluorouracil–induced Aggravation of Breast Cancer Metastasis to Lung
Authors: Sasaki Soichiro , Baba Tomohisa , Muranaka Hayato , Tanabe Yamato , Takahashi Chiaki , Matsugo Seiichi , Mukaida Naofumi 
Published: April 2018

AbstractAdjuvant chemotherapy is used for human breast cancer patients, even after curative surgery of primary tumor, to prevent tumor recurrence primarily as a form of metastasis. However, anticancer drugs can accelerate metastasis in several mouse metastasis models. Hence, we examined the effects of postsurgical administration with 5-fluorouracil (5-FU), doxorubicin, and cyclophosphamide, on lung metastasis process, which developed after the resection of the primary tumor arising from the orthotopic injection of a mouse triple-negative breast cancer cell line, 4T1. Only 5-FU markedly increased the numbers and sizes of lung metastasis foci, with enhanced tumor cell proliferation and angiogenesis as evidenced by increases in Ki67-positive cell numbers and CD31-positive areas, respectively. 5-FU–mediated augmented lung metastasis was associated with increases in intrapulmonary neutrophil numbers and expression of neutrophilic chemokines, Cxcl1 and Cxcl2 in tumor cells, with few effects on intrapulmonary T-cell or macrophage numbers. 5-FU enhanced Cxcl1 and Cxcl2 expression in 4T1 cells in a NFκB-dependent manner. Moreover, the administration of a neutrophil-depleting antibody or a Cxcr2 antagonist, SB225002, significantly attenuated 5-FU–mediated enhanced lung metastasis with depressed neutrophil infiltration. Furthermore, infiltrating neutrophils and 4T1 cells abundantly expressed prokineticin-2 (Prok2) and its receptor, Prokr1, respectively. Finally, the administration of 5-FU after the resection of the primary tumor failed to augment lung metastasis in the mice receiving Prokr1-deleted 4T1 cells. Collectively, 5-FU can enhance lung metastasis by inducing tumor cells to produce Cxcl1 and Cxcl2, which induced the migration of neutrophils expressing Prok2 with a capacity to enhance 4T1 cell proliferation. Mol Cancer Ther; 17(7); 1515–25. ©2018 AACR.
Cerebrovascular Accidents During Mechanical Circulatory Support
Authors: Izzy Saef , Rubin Daniel B. , Ahmed Firas S. , Akbik Feras , Renault Simone , Sylvester Katelyn W. , Vaitkevicius Henrikas , Smallwood Jennifer A. , Givertz Michael M. , Feske Steven K. 
Published: April 2018

Background and Purpose—
Left ventricular assist devices (LVADs) have emerged as an effective treatment for patients with advanced heart failure refractory to medical therapy. Post-LVAD strokes are an important cause of morbidity and reduced quality of life. Data on risks that distinguish between ischemic and hemorrhagic post-LVAD strokes are limited. The aim of this study was to determine the incidence of post-LVAD ischemic and hemorrhagic strokes, their association with stroke risk factors, and their effect on mortality.

Methods—
Data are collected prospectively on all patients with LVADs implanted at Brigham and Women’s Hospital. We added retrospectively collected clinical data for these analyses.

Results—
From 2007 to 2016, 183 patients (median age, 57; 80% male) underwent implantation of HeartMate II LVAD as a bridge to transplant (52%), destination therapy (39%), or bridge to transplant candidacy (8%). A total of 48 strokes occurred in 39 patients (21%): 28 acute ischemic strokes in 24 patients (13%) and 20 intracerebral hemorrhages in 19 patients (10.3%). First events occurred at a median of 238 days from implantation (interquartile range, 93–515) among those who developed post-LVAD stroke. All but 9 patients (4.9%) were on warfarin (goal international normalized ratio, 2–3.5) and all received aspirin (81–325 mg). Patients with chronic obstructive pulmonary disease were more likely to have an ischemic stroke (odds ratio, 2.96; 95% confidence interval, 1.14–7.70). Dialysis-dependent patients showed a trend toward a higher risk of hemorrhagic stroke (odds ratio, 6.31; 95% confidence interval, 0.99–40.47). Hemorrhagic stroke was associated with higher mortality (odds ratio, 3.92; 95% confidence interval, 1.34–11.45) than ischemic stroke (odds ratio, 3.17; 95% confidence interval, 1.13–8.85).

Conclusions—
Stroke is a major cause of morbidity and mortality in patients on LVAD support. Chronic obstructive pulmonary disease increases the risk of ischemic stroke, whereas dialysis may increase the risk of hemorrhagic stroke. Although any stroke increases mortality, post-LVAD hemorrhagic stroke was associated with higher mortality compared with ischemic stroke.