bluuebunny/crossref_metadata_2025_split huggingface dataset:
Мультицентровое рандомизированное исследование пролонгированной антибиотикопрофилактики при цистэктомии в клиниках, работающих по протоколу раннего послеоперационного восстановления (ERAS)
Authors: Беркут М. В. , Беляев А. М. , Галунова Т. Ю. , Тяпкин Н. И. , Рева С. А. , Носов А. К. 
Published: November 2024

Стандартная 24‑часовая антибиотикопрофилактика (АП) широко используется для минимизации риска осложнений с инфекцией в течение 30 дней после радикальной цистэктомии (РЦЭ). Однако значительное разнообразие протоколов, комбинаций антибактериальных препаратов и продолжительность назначения не предотвращают высокий уровень инфекционных осложнений после операции, частота которых может достигать 37–67 %.Целью настоящего исследования является оценка риска развития инфекционных осложнений в период 30 дней после РЦЭ в хирургическом стационаре, работающем по протоколу ERAS при сравнении стандартного 24‑часового режима АП в сравнении с 120‑часовым (пролонгированный режим).Материалы и методы: 92 пациента были рандомизированы в соотношении 1:1 на стандартный 24‑часовой режим антибиотиков (стандартная АП) и пролонгированный режим с применением меропенема в течении 120 часов. В данной работе были рассмотрены вторичные конечные точки — динамика биомаркеров системного воспалительного ответа и частота развития приобретенных карбапенемаз на фоне пролонгированной АП.Результаты: Исходные и периоперационные характеристики были сбалансированы между группами. Анализ системного воспаления не выявил значимых изменений уровня системного воспалительного ответа в динамике на 1‑е и 14‑е сутки после операции (р = 0,791 и р = 0,219). При этом динамика уровня СРБ была менее интенсивная в группе пролонгированной АП, особенно на 1‑е сутки после операции (р = 0,020). Чаще всего выявлялись карбопенемазы классов B и D, ассоциированные с повышенным риском инфекционных осложнений. Кроме того, в группе пролонгированной АП отмечено снижение встречаемости карбопенем‑продуцирующих штаммов (p = 0,111), где регрессионный анализ показал связь между выявлением карбопенемаз и развитием инфекционных осложнений (ОР 2,73, p = 0,024).Выводы: Данное проспективное рандомизированное исследование демонстрирует, что пролонгированная антибиотикопрофилактика эффективно снижает 30‑дневные послеоперационные осложнения после радикальной цистэктомии, при этом обеспечивая менее выраженный подъем С‑реактивного белка и не увеличивая частоту антибиотикорезистентности, что открывает новые горизонты для обновления протоколов в онкохирургии.
Cuerpo-tierra entre colapsos y Refugios
Authors: Azambuja Katiuska , Carneiro Maristela , Molina Saldarriaga Mónica Lucía 
Published: November 2024

Refugio es una obra performativa realizada con mujeres en la ciudad de Várzea Grande, Brasil, en 2023. La propuesta se desarrolla en etapas poéticas, compuestas por dibujos, sonidos, cartas y un videoarte. La reflexión parte del texto La sociedad del cansancio (Han, 2015), en relación con el colapso climático. A partir de ello, se pregunta: ¿Cómo tener una vida radicalmente viva?, evocando el pensamiento de Ailton Krenak (2020) y Dènetém Bona (2020). El objetivo fue activar la escucha del cuerpo-tierra, expresión de la artista Ana Mendieta, en relación con la idea de la tierra-cuerpo, junto con el concepto original de aisthesis, con el cual, se construye subjetividades decoloniales (Mignolo, 2011). Estas ideas sobrepasan la dualidad cultura - naturaleza (Mignolo, 2017), y retoman la percepción de la naturaleza como ser vivo y las relaciones milenarias que sustentan la vida (Walsh, 2008). Como herramienta metodológica, se escogió la cartografía, por ser un antimétodo que exige una atención despojada de formación de subjetividades; la necesidad de acompañar procesos, por el contrario, exige habitar y reconocer un territorio existencial (Barros & Kastrup, 2015). Las participantes revelaron una percepción de ritmo/espacio más atento y una conciencia sobre la escucha del cuerpo-tierra y la tierra-cuerpo.
Performance Evaluation for Positive Displacement Motors by Combining Fluid-Structure Interaction Simulations and Experiments
Authors: Kuang Yuchun , Zhou Jingpei 
Published: November 2024

Summary
The aim of this study is to propose a method for evaluating the performance of positive displacement motors (PDMs) from the perspective of robustness and energy conversion efficiency. Monitoring contact force and leakage is challenging when a steel rotor driven by liquid undergoes planetary motion within the rubber lining of the stator. A 3D model with a nominal diameter of 172 mm is established, along with a flow field of the same outer diameter as the stator. The constitutive parameters are determined according to the two-parameter Mooney-Rivlin model. After merging the overlapping nodes of the rubber lining and steel housing, the degrees of freedom are constrained from the exterior of the stator. Four-step loads are applied in ascending order. The average difference in overall efficiency between the experimental results and the simulation of the same model PDM is 1.58%. The sequential simulations include three sets of nine variables: lead (800 mm, 914.4 mm, 1200 mm), interference fit (0.1 mm, 0.2 mm, 0.3 mm), and rubber hardness (70 HA, 80 HA, 85 HA). Contact force differentials on each sealing band are calculated before and after stable operation. The reliability and stability of the cavities are assessed using the mean and variance of the force differentials. Efficiencies after each load stabilization are calculated to compare performance. For loads other than 200 N·m, the overall efficiency of the PDM with the optimized parameter combination increased by 2.98%, 0.27%, and 2.32%, respectively. Finally, this parameter combination is applied to a curved profile PDM. For loads other than 200 N·m, the overall efficiency increased by 3.22%, 0.89%, and 5.14%. This method, which combines the fluid-structure interaction (FSI) with an analysis of robustness and efficiencies, can be used to analyze the strengths and weaknesses of different parameter PDMs and to assess the performance of different profile motors.
Mechanism of Subsurface Deformation Transmission and Geomechanical Response Inversion in Carbon Capture and Storage Project: Integrating Small Baseline Subset-Interferometric Synthetic Aperture Radar, Mechanical Experiments, and Numerical Simulation
Authors: Ye Xiaoqi , Yu Ziwang , Xu Tianfu , Zhang Yanjun , Guo Liangliang 
Published: November 2024

Summary
In the context of carbon capture and storage (CCS) engineering, ensuring the stability of the caprock is paramount to mitigating CO2 leakage, thus constituting a pivotal engineering challenge in CO2 geological sequestration. With the injection of CO2, pore pressure accumulates within the reservoir, bringing forth risks including diminished effective stress within the formation, surface deformation, occurrence of microseismic events, and potential caprock failure. Therefore, it is necessary to explore the geomechanical issues in CCS projects. This study focuses on the Daqingzijing in the Jilin Oilfield as the study area, utilizing the small baseline subset (SBAS)-interferometric synthetic aperture radar (InSAR) method to conduct a deformation time-series analysis in the well group area under injection and production conditions. The results reveal variations in deformation sensitivity among the sites, with surface displacements correlated to fluid injection and production, demonstrating temporal delays. At the H79 North block, the time effect is relatively minimal, with rapid propagation of formation deformation. Surface displacement in the H46 block appeared 4 months later than behind cumulative fluid volume changes. By conducting triaxial creep tests on shallow mudstone samples from the Songliao Basin under various triaxial stress states, a constitutive creep equation for caprock rocks was obtained. The numerical models of elastic and creep constitutive equations were established. The results show that the creep model exhibits superior accuracy by comparing with InSAR monitoring data (the root mean square error values of elastic and creep constitutive geomechanical models were 6.7 mm and 1.7 mm, respectively). Additionally, based on the experimental and simulation results, this study explores the transfer mechanisms of formation deformation and the inverse relationship between deformation and pore pressure. This study provides theoretical support for the geomechanical safety analysis in corresponding CCS projects.
Mechanisms characterizing DOM migration in coal mining subsidence areas
Authors: Xu Jing , Gao liangmin , Yang Jinxiang , Xia Jieyu , Song Han , Liu Yanjun , Zhang Kai , Wu Lin , Shu Xin 
Published: November 2024

Abstract

The migration mechanism of DOM in water, soil and sediment t from the coal mining subsidence area of Huabei Xutuan Mine were investigated using three-dimensional fluorescence-parallel factor analyses (EEMs-PARAFAC), self-organized neural networks (SOM), and Fourier infrared spectroscopy (FT-IR). According to the SOM model, Neuron 13, the representative of soil, and Neuron 36, representative of sediment, were categorized as protein-like, whereas Neuron 27, representative as water, was categorized as humus-like. Based on the PARAFAC results, water C1 and C2, soil C1, and sediment C1 and C3 were humus-like fractions, and water C3, soil C2, C3, and sediment C2 were protein-like fractions. The DOM in the water, soil, and sediment samples was mainly endogenous and had a low degree of humification. The FT-IR results showed that the DOM in the water body had more humus-like functional groups related to microbial decomposition, and the soil around the subsidence area was sunken and flooded to form the sediment. Therefore, the soil and sediment samples showed more protein moieties related to biological activities.
The serum cholesterol level of choledocholithiasis patients was significantly lower than that of healthy people
Authors: Li Linzhen , Hu Tulan , Wang Di 
Published: November 2024

Abstract

Background and purpose
Choledocholithiasis is a common disease. The specific pathogenesis of choledocholithiasis is still unclear. The objective of this study was to investigate whether serum cholesterol level is related to the incidence of choledocholithiasis.
Patients and Methods:
A total of 169 choledocholithiasis patients were include in this study. We investigated whether total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), TC/TG, LDL/HDL in choledocholithiasis patients differed from that in healthy people.
Results
There were 64 male and 105 female patients. There were no statistically significant difference in age (P = 0.927), TC/TG (P = 0.516) and LDL/HDL (P = 0.139) between the two groups. There were significant differences in TC (P<0.001), TG (P = 0.006), HDL (P<0.001) and LDL (P = 0.001) between the two groups. The TC, TG, HDL, LDL in the choledocholithiasis patients group were significantly lower than that in the healthy population. In the subgroup analysis, we further investigate whether above parameters in choledocholithiasis patients differed from that in healthy people by gender. There was no significant difference in TG (P = 0.182), TC/TG (P = 0.982), LDL/HDL (P = 0.392) between the male choledocholithiasis patients group and the male healthy population group. There were significant differences in TC (P = 0.001), HDL (P = 0.014) and LDL (P = 0.026) between the two groups. There were no significant difference in TC/TG (P = 0.590), LDL/HDL (P = 0.116) between the female choledocholithiasis patients group and the female healthy population group. There were significant differences in TC (P<0.001), TG (P = 0.016), HDL (P<0.001) and LDL (P = 0.009) between the two groups.
Conclusions
The serum TC, HDL and LDL in choledocholithiasis patients were significantly lower than those in healthy people, and TG in female patients was significantly lower than that in healthy people, but there was no difference in male patients.
The required experience of open pancreaticoduodenectomy before becoming a specialist inhepatobiliary and pancreatic surgeons: A multicenter, cohort study of 334 open pancreaticoduodenectomies
Authors: Fuji Tomokazu , Umeda Yuzo , Takagi Kosei , Hioki Masayoshi , Yoshida Ryuichi , Endo Yoshikatsu , Yasui Kazuya , Nobuoka Daisuke , Mitsuhashi Toshiharu , Fujiwara Toshiyoshi 
Published: November 2024

Abstract

Background
Open pancreaticoduodenectomy (OPD) is an essential surgical procedure for expert hepato-biliary-pancreatic (HBP) surgeons. However, there is no standard for how many surgeries must be performed by a surgeon in training before they are considered to have enough experience to ensure surgical safety.
Methods
Cumulative Sum (CUSUM) analysis was performed using the surgical data of OPDs performed during the training period of board-certified expert surgeons of the Japanese Society of Hepato-Biliary-Pancreatic Surgery.
Results
Fourteen HBP surgeons participated in this study and performed 334 OPDs during their training period. The median (interquartile range) values for operative time, blood loss, and length of hospital stay were 455 (397–519) minutes, 450 (234–-716) ml, and 28 (21–38) days, respectively. CUSUM analysis showed inflection points at 20 surgeries performed for operative time. After 20 procedures, operative time was significantly shorter (461 min vs. 425 min, p = 0.021) and blood loss was significantly lower (470 ml vs. 340 ml, p = 0.038). No significant differences between within 20 and after 21 procedures were found in the complication rate (53% vs. 48%, p = 0.424) and rate of in-hospital deaths (1.5% vs.1.4%. p = 0.945)
Up to 20 surgeries, PDAC and another malignant tumor had longer operative time than benign/low malignant diseases (486 min vs 472 min vs 429 min, p < 0.001), and higher blood loss (500 ml vs 502 ml vs 355 ml, p < 0.001). Mortality rate was higher at PDAC cases (5% vs 0% vs 0%, p = 0.01). After the 21 procedures, these outcomes were improved and no differences in by primary disease were observed.
Multivariable analysis showed that within 20 surgeries were independent risk factors of longer operative time (HR2.6, p = 0.013) and higher blood loss (HR2.0, p = 0.049).
Conclusions
To stabilize the surgical outcome of OPD for malignant disease, at least 20 surgeries should be performed at a certified institution during surgeon training.
Trial registration: Clinical trial number: not applicable
GB10, a best-in-class antibody fusion protein targeting VEGF/Ang-2, exhibits promising therapeutic efficacy for neovascular eye diseases
Authors: Wei Xiling , Qiu Yuxin , Shang Wei , Zhang Xiangling , Yang Wenjie , Yang Chengyong , Chen Xi , Li Huiming , Qin Suofu 
Published: November 2024

Abstract

Background
In clinical practice, anti-vascular endothelial growth factor (VEGF) therapies have been successfully applied to patients with neovascular eye diseases. However, unmet clinical needs have not yet been fully addressed, as about 20% of patients do not response to anti-VEGF monotherapies, meanwhile, the high frequency of intravitreal (IVT) injections imposes a significant burden on patients. To overcome these challenges, we developed a novel antibody fusion protein GB10 consisting of a VEGF-Trap and an anti-angiopoietin 2 (Ang-2) variable heavy domain of heavy-chain antibody (VHH) to inhibit the pro-angiogenic pathways of VEGF and Ang-2 simultaneously for enhanced and more enduring efficacy. The activity and developability of GB10 were characterized.
Methods
We first explored two categorical formats for molecular construction and selected the format that demonstrated the best activity and CMC-related properties for the generation of GB10. Subsequently, we evaluated the multi-targeting capability of GB10 using bridging enzyme-linked immunosorbent assay (ELISA) and bio-layer interferometry (BLI), followed by a side-by-side comparison of the in vitro activities of GB10 and faricimab, the only marketed bispecific antibody for neovascular eye diseases, through assays such as VEGF reporter assay, human umbilical vein endothelial cells (HUVEC) proliferation, Ang-2 blocking ELISA, and Tie-2 phosphorylation. The in vivo efficacy of GB10 and faricimab was next evaluated using a non-human primate model of laser-induced choroidal neovascularization (CNV). Finally the developability of GB10 was evaluated by intraocular pharmacokinetics and stress test.
Results
GB10 bound VEGF and Ang-2 simultaneously with high affinity, and exhibited superior activity in vitro in inhibiting the VEGF and Ang-2 signaling pathways compared to faricimab. In vivo, GB10 demonstrated greater efficacy and durability compared to faricimab in a CNV model. GB10 also possessed a longer half-life in vitreous measured in a rabbit model. Moreover, GB10 showed excellent injectability and stability at a high-concentration of 140 mg/mL.
Conclusions
The superb efficacy and favorable developability profile make GB10 a potential best-in-class therapy for patients with neovascular eye diseases, warranting further evaluation in clinical settings.
Generative deep learning enables the discovery of phosphorylation-suppressed STAT3 inhibitors for non-small cell lung cancer therapy
Authors: Cai Weiji , Jiang Beier , Yin Yichen , Ma Lei , Li Tao , Chen Jing 
Published: November 2024

Abstract

The discovery of phosphorylation-suppressed inhibitors for Signal Transducer and Activator of Transcription 3 (STAT3) presents a novel therapeutic strategy for non-small cell lung cancer (NSCLC). Despite the pivotal roles of STAT3 in cancer progression, effective inhibitors remain limited, especially for efficiently suppressing phosphorylation at Try705. This study harnesses generative deep learning to develop a model for de novo design of STAT3 inhibitors that selectively target the phosphorylated form and subsequentially induce cellular apoptosis. Initially, we constructed a generative model utilizing a generative deep learning with transfer learning and virtual screening, trained on existing STAT3 inhibitor datasets to explore the chemical space. We generated a diverse library of candidate compounds, which were subsequently screened through molecular docking and pharmacophore modeling, identifying several promising inhibitors. Compared with HG106, HG110 molecule can efficiently suppress phosphorylation of STAT3, and suppress the nucleus translocation of STAT3 in H441, which stimulated by IL6 pro-inflammatory factor. Rigorous molecular dynamics (MD) simulations were performed to evaluate the stability and interaction profiles of selected candidates within the STAT3 binding site. Among the top candidates, compounds HG106 and HG110 exhibited superior binding affinities compared to known STAT3 inhibitors. The MD simulations confirmed stable conformations and favorable interactions with key residues in the binding pocket, indicating potential for in vivo efficacy. This study demonstrates the power of generative deep learning in accelerating the identification of novel phosphorylation-suppressed STAT3 inhibitors, providing a promising direction for NSCLC therapy.
In silico evaluation of erythrinic alkaloids from Erythrina verna in human crystallographic model GABAA R-β3 (4COF) and GABAA R-α1-β2-γ2 (6X3X)
Authors: Nascimento Marcelo Henrique Santana , Paula Fávero Reisdorfer , Rosa Marcelo Barcellos , Ugalde Gustavo Andrade , Bairros André Valle 
Published: November 2024

Abstract

Erythrina verna, known as mulungu, is a medicinal plant recognized by anxiolytic effects attributed to erythrinic alkaloids. This research aims to enhance our understanding of the mechanism of these alkaloids through in silico studies and explore their potential interaction with GABAA sub-receptors. Molecular modeling and docking simulations were performed to evaluate the conformational structure and interaction energies of the molecules as well as toxicity prediction. Multivariate statistical analyses were utilized to highlight the influence binding affinity. The results indicate strong interactions of the erythrinic alkaloids with key amino acid residues from GABAA receptor while multivariate analysis revealed significant interaction patterns for erythradine and erysortrine. Mutagenic, tumorigenic, irritant, or reproductive risks were not observed in these molecules. So, erythrinic alkaloids exposure demonstrating safety and efficacy as a natural anxiolytic. It also contributes to our understanding of erythrinic alkaloids’ pharmacological effects and supports the further development of natural anxiolytics for therapeutic applications.
A unique lithospheric origin for a diamond from the Rio Sorriso area, Brazil
Authors: Angellotti Antonio , Marras Giulia , Morana Marta , Chariton Stella , Stopponi Veronica , Medeghini Laura , Romano Claudia , Correale Alessandra , Bindi Luca , Kaminsky Felix , Stagno Vincenzo 
Published: November 2024

Abstract

Diamonds provide unique information on the deep Earth’s mantle through the investigation of entrapped minerals and fluids from which pressure-temperature-oxygen fugacity are determined. In this study, we investigated a diamond from the Rio Sorriso area, Juina (Brazil), a site known for the high abundance of discovered sublithospheric diamonds. The studied diamond contains both colorless and greenish optically visible inclusions of Cr-diopside, high-Mg olivine, and enstatite. Thermobarometric estimates of the polished and entrapped inclusions suggest that the diamond likely formed between 4 and 5 GPa, and in the T range 1050–1150°C; in contrast, major and trace elements data from one polished clinopyroxene provide evidence of interaction between the local peridotite and a Na-rich carbonated melt, the growth medium from which the diamond crystallized. Our study, thus, demonstrates that diamonds from underneath the Amazonian craton did not originate solely at lower mantle depths but also within a metasomatized lithospheric mantle.
Integrated apparent trait and gene expression reveal enzymatic browning mechanism in fresh Pinellia ternata
Authors: Tao Jing , Sun Jiayi , Liang Honghong , Li Ruoshi , Tang Min , Wang Yaodeng , Gan Zhengkun , Tang Shunyu , Ai Li , Jiang Guihua 
Published: November 2024

Abstract

The medicinal plant, Pinellia ternata (Thunb.) Breit., known as Banxia, is extensively used in traditional Chinese medicine. However, it is prone to enzymatic browning during natural drying, influencing the color quality and reducing commercial value. This study systematically investigated the molecular mechanism of enzymatic browning in P. ternata by monitoring surface color, polyphenol oxidase (PPO) activity, and gene expression by colorimeter, spectrophotometry, and qPCR during different browning periods. The study observed a gradual decrease in lightness (L*), yellowness (b*), and browning index (BI), alongside an increase in redness (a*) and total color difference (ΔE) during the drying process. Notably, a critical period for browning was identified between 6 to 8 hours of drying. Additionally, an upregulation in PPO activity and gene expression was noted during the browning period, with the PPO gene expression strongly and positively correlating with b* and negatively with a*, further substantiating the significant role of PPO in the degree of browning. By integrating phenotypic traits with gene expression analysis, this study unraveled the enzymatic browning mechanism in fresh P. ternata, laying the groundwork for future research aimed at mitigating enzymatic browning in medicinal plants such as P. ternata.