bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gene expression profiling of the olfactory tissues of sex-separated and sex-combined female and male mice
Authors: Santoro Stephen W. , Jakob Susanne 
Published: December 2018

AbstractOlfactory experience can alter the molecular and cellular composition of chemosensory neurons within the olfactory sensory epithelia of mice. We sought to investigate the scope of cellular and molecular changes within a mouse’s olfactory system as a function of its exposure to complex and salient sets of odors: those emitted from members of the opposite sex. We housed mice either separated from members of the opposite sex (sex-separated) or together with members of the opposite sex (sex-combined) until six months of age, resulting in the generation of four cohorts of mice. From each mouse, the main olfactory epithelium (MOE), vomeronasal organ (VNO), and olfactory bulb (OB) were removed and RNA-extracted. A total of 36 RNA samples, representing three biological replicates per sex/condition/tissue combination, were analyzed for integrity and used to prepare RNA-seq libraries, which were subsequently analyzed via qPCR for the presence of tissue- or sex-specific markers. Libraries were paired-end sequenced to a depth of ~20 million fragments per replicate and the data were analyzed using the Tuxedo suite.
The Petroleum Ether Extract from Hypericum perforatum Root Cultures Exhibits Potent Antiproliferative Activity in Human Keratinocytes and Fibroblasts
Authors: Gaid Mariam , Füller Jendrik , Müller-Goymann Christel 
Published: December 2018

AbstractThe hyperforin content of Hypericum perforatum herb was repeatedly reported to be responsible for a multitude of pharmacological activities. Our recent report about the hyperforin accumulation in in vitro root cultures of H. perforatum provides an alternative perspective to achieve constant product quality and to serve the rapidly growing market. In this study, the antiproliferative effect of a petroleum ether extract from the in vitro root cultures was investigated. When normalized to 1 µM hyperforin content, the extract reduced the viability of human keratinocytes (HaCaT) and human dermal fibroblast monolayers to 33 and 36%, respectively, after 72 h of incubation. A cytotoxicity assay and live-dead cell staining confirmed that the extract lacked a cytotoxic effect and that the reduction in cell viability was mainly due to the antiproliferative activity. Application of the 1 µM hyperforin-normalized extract to a 3D artificial skin construct significantly reduced the proliferation of HaCaT in the presence of fibroblasts. This effect was proved by the reduction in thickness of the epidermal construct from 100 µm (control) to 17 µm (treated). Notably, 1 µM pure hyperforin lacked effectiveness in both monolayer cultures and 3D artificial skin constructs. Nor were fractions of the extract containing colupulone and xanthones active. The combination of these constituents also failed to reassemble the antiproliferative activity, which indicates a synergistic role of yet unidentified components present in the extract. Our findings may introduce H. perforatum root cultures as a novel lead system for the treatment of hypertrophic scars.
A New Technique for Reconstruction of Medium-Sized Eyelid Defects (A Modification of Tessier Nasojugal Flap)
Authors: Yousefiazar Awat , Hassanzadazar Mohsen 
Published: December 2018

AbstractEyelid reconstructions are considered challenging surgical procedures, which are mostly performed due to trauma, tumor resections, and congenital defects. Several techniques can be used to reconstruct the lower eyelid defects. In this study, the authors aimed to introduce a new single-step technique for the reconstruction of medium-sized lower eyelid defects while preventing ectropion. A modified nasojugal flap was designed and harvested after making a complete incision. A dermal extension from the nasolabial area was added to the end of the nasojugal flap. A tunnel was created under the orbicularis oculi muscle, and the dermal tail was passed through this tunnel toward the lateral canthus. The flap was fixed in its new position by suturing. The defect in the nasojugal area was repaired by bilateral advancement of the surrounding tissue. The recovery of the patients was followed up for 6 months, which included assessment of the eyelid position and donor site morbidity. A total of four patients with an age range of 64 ± 8 years who suffered from basal cell carcinoma were treated using the technique introduced in this study. No ectropion or abnormal eyelid position nor donor site morbidity was observed during the follow-up period. Reconstruction of a medium-sized lower eyelid defect using nasolabial flap with local modification combined with orbital canthus fixation resulted in appropriate functional and cosmetic outcomes and limited the risk of complications and morbidity.
Proximal Hamstring Injuries: Review of Operative and Nonoperative Management
Authors: Duchman Kyle , Amendola Ned , Buckwalter V Joseph 
Published: December 2018

AbstractWhile uncommon, proximal hamstring injuries can result in significant pain and dysfunction, while also posing considerable treatment dilemmas due to the spectrum of injury that exists. Recent literature suggests that outcomes are improved with acute surgical repair of complete proximal hamstring avulsions, highlighting the importance of timely management of these unique injuries. While chronic repair or reconstruction can result in predictable improvements in pain and function postoperatively, the results are less predictable than acute surgical repair. Nonoperative management should be considered as the initial treatment strategy for patients with proximal hamstring tendinopathy or partial tears, as delayed surgical intervention in this setting has not been shown to significantly affect outcomes. Although current evidence is limited, evolving therapeutic techniques, including biologic injections and endoscopic repair techniques, need to be carefully evaluated to determine their role moving forward. This review aims to provide updated information on the relevant anatomy, mechanism of injury, diagnosis, and management of proximal hamstring injuries, with a specific focus on surgical indications, techniques, and outcomes.
A Case of Congenital Bronchial Pulmonary Arterial Malformation Successfully Treated by Embolization
Authors: Chu Ferdinand , Sit Ko , Kwok King 
Published: December 2018

AbstractIdiopathic bronchial pulmonary arterial malformation (BPAM) is a very rare condition. The authors present a case of BPAM in which a right bronchial artery communicates with a main upper lobe branch of the right pulmonary artery. It was successfully treated by embolization in one setting. The patient remained asymptomatic and well during the follow-up period. The authors therefore conclude that if the embolic material/device is carefully chosen, it is a safe and effective means of treating BPAM.
Schizophrenia Exhibits Bi-directional Brain-Wide Alterations in Cortico-Striato-Cerebellar Circuits
Authors: Ji Jie Lisa , Diehl Caroline , Schleifer Charles , Tamminga Carol A , Keshavan Matcheri S , Sweeney John A , Clementz Brett A , Hill S Kristian , Pearlson Godfrey , Yang Genevieve , Creatura Gina , Krystal John H , Repovs Grega , Murray John , Winkler Anderson , Anticevic Alan 
Published: December 2018

Abstract
Distributed neural dysconnectivity is considered a hallmark feature of schizophrenia (SCZ), yet a tension exists between studies pinpointing focal disruptions versus those implicating brain-wide disturbances. The cerebellum and the striatum communicate reciprocally with the thalamus and cortex through monosynaptic and polysynaptic connections, forming cortico-striatal-thalamic-cerebellar (CSTC) functional pathways that may be sensitive to brain-wide dysconnectivity in SCZ. It remains unknown if the same pattern of alterations persists across CSTC systems, or if specific alterations exist along key functional elements of these networks. We characterized connectivity along major functional CSTC subdivisions using resting-state functional magnetic resonance imaging in 159 chronic patients and 162 matched controls. Associative CSTC subdivisions revealed consistent brain-wide bi-directional alterations in patients, marked by hyper-connectivity with sensory-motor cortices and hypo-connectivity with association cortex. Focusing on the cerebellar and striatal components, we validate the effects using data-driven k-means clustering of voxel-wise dysconnectivity and support vector machine classifiers. We replicate these results in an independent sample of 202 controls and 145 patients, additionally demonstrating that these neural effects relate to cognitive performance across subjects. Taken together, these results from complementary approaches implicate a consistent motif of brain-wide alterations in CSTC systems in SCZ, calling into question accounts of exclusively focal functional disturbances.
Pain relief after orthodontic archwire installation—a comparison between intervention with paracetamol and chewing gum: a randomized controlled trial
Authors: Alshammari Abdulrahman K , Huggare Jan 
Published: December 2018

Summary

Background
Pain and discomfort are main concerns at the commencement of orthodontic treatment. It may have negative impact on compliance or even discourage patients from being treated. Orthodontic pain can be alleviated by paracetamol or use of chewing gum. However, studies comparing their effectiveness are scarce.

Objectives
To compare the effectiveness of paracetamol versus chewing gum for pain alleviation and to investigate the frequency of bracket loss during the first day of fixed orthodontic treatment.

Trail design
Prospective randomized multicentre clinical trial.

Methods
Sixty patients (28 boys and 32 girls), between 12 and 18 years of age were randomly allocated either to take paracetamol (31 patients) or to use chewing gum (29 patients). After one arch bonding and insertion of the initial archwire, the patients rated the sensation of pain on a visual analogue scale with the jaw at rest and when biting after 6 hours (T1), at bedtime (T2), and the next morning (T3). Paracetamol (1000 mg) was taken 1 hour and chewing gum was used 10 minutes prior to pain rating at T2 and T3. A simple method of randomization was used in this study, and blinding of subjects and the operators to the type of intervention was not possible because of the nature of the treatments.

Results
There was no detectable difference in pain sensation between the groups at T1. At T2 and T3 the chewing gum group displayed higher mean values than the paracetamol group but when adjusting for age, gender, and mode of pain registration, there was no significant difference between the groups. There was no bracket loss in the chewing gum group, whereas two patients in the paracetamol group lost two brackets.

Conclusions
The effect of chewing gum and paracetamol for initial orthodontic pain relief seems equivalent. Short term use of chewing gum is not a risk factor for bracket loss.

Trial registration
This study was not registered.
Evolution of DNA Methylation in Papio Baboons
Authors: Vilgalys Tauras P , Rogers Jeffrey , Jolly Clifford J , Baboon Genome Analysis  , Mukherjee Sayan , Tung Jenny 
Published: December 2018

AbstractChanges in gene regulation have long been thought to play an important role in primate evolution. However, although a number of studies have compared genome-wide gene expression patterns across primate species, fewer have investigated the gene regulatory mechanisms that underlie such patterns, or the relative contribution of drift versus selection. Here, we profiled genome-scale DNA methylation levels in blood samples from five of the six extant species of the baboon genus Papio (4–14 individuals per species). This radiation presents the opportunity to investigate DNA methylation divergence at both shallow and deeper timescales (0.380–1.4 My). In contrast to studies in human populations, but similar to studies in great apes, DNA methylation profiles clearly mirror genetic and geographic structure. Divergence in DNA methylation proceeds fastest in unannotated regions of the genome and slowest in regions of the genome that are likely more constrained at the sequence level (e.g., gene exons). Both heuristic approaches and Ornstein–Uhlenbeck models suggest that DNA methylation levels at a small set of sites have been affected by positive selection, and that this class is enriched in functionally relevant contexts, including promoters, enhancers, and CpG islands. Our results thus indicate that the rate and distribution of DNA methylation changes across the genome largely mirror genetic structure. However, at some CpG sites, DNA methylation levels themselves may have been a target of positive selection, pointing to loci that could be important in connecting sequence variation to fitness-related traits.
Sustained investment surges
Authors: Libman Emiliano , Montecino Juan Antonio , Razmi Arslan 
Published: December 2018

AbstractExisting empirical studies have mainly focused on determinants of average investment levels. Instead, we investigate episodes of accelerated capital stock growth having a duration of eight years or longer. We find that episodes are relatively common, even in low-growth regions, but more so in middle-income and Asian countries. After identifying 175 episodes between 1950 and 2014, we employ probit analysis to explore their characteristics. Turning points in investment tend to be preceded by macroeconomic stability, real exchange rate undervaluation, and net capital outflows (especially portfolio outflows). We also find a negative correlation with the capital to output ratio and per capita GDP, and a positive correlation with a human capital index. Investment surges tend to be associated with changes in the trade balance and, to a (statistically) weaker extent, with structural change.
The Limits of Federation-wide Political Parties in Ensuring Federal Stability: Nigeria under the Peoples Democratic Party
Authors: Okpanachi Eyene 
Published: December 2018

AbstractSince Nigeria’s return to electoral rule in 1999, the People’s Democratic Party (PDP) ruled the country as the majority party at both levels of its federal system until 2015. However, despite this dominance, the relationship between presidents and governors was often so divisive that the instability within the party threatened the stability of not only the ruling governments, but also the federation as a whole and undermined its productivity. This article examines this anomaly against the background of scholarship emphasizing the crucial role of federation-wide parties in fostering smooth intergovernmental bargains and in facilitating federal stability. It argues that Nigeria’s recent experience provides counterevidence of this theory and discusses the mutually reinforcing contextual factors that might have influenced this development, focusing on the PDP’s norms and the country’s intergovernmental fiscal structure.
Local Actions, National Policies and International Knowledge: Family Planning and Statistical Practices in Taiwan (1949–1980s)
Authors: Lin Yi-Tang 
Published: December 2018

Summary
Using an approach based on the sociology of quantification, this article illustrates how actors utilised statistics when importing family planning to Taiwan and exporting their experience to international policy makers. The functions of statistics—producing knowledge and making policies—assisted the implementation of international programmes in Taiwan, where any actions leading to a population decrease were prohibited in the 1950s. The Population Council and Taiwanese officials first secured the provincial government’s tacit consent by claiming the programme to be an experiment in general population policy rather than one focused on the insertion of intra-uterine devices (IUDs). They went on to win the central government’s endorsement in 1964 by presenting IUD insertions as tools for achieving the ideal population size for economic development. Finally, experts packaged and repackaged the Taiwan programme as a success by wielding locally-collected statistics, reframing the programme to fit the conclusions of international research at the time.
Somatic uniparental disomy mitigates the most damagingEFL1allele combination in Shwachman-Diamond syndrome
Authors: Lee Sangmoon , Shin Chang Hoon , Lee Jawon , Jeong Seong Dong , Hong Che Ry , Kim Jun-Dae , Kim Ah-Ra , Son Soo Jin , Kokhan Oleksandr , Yoo Taekyeong , Ko Jae Sung , Sohn Young Bae , Kim Ok-Hwa , Ko Jung Min , Cho Tae-Joon , Wright Nathan T. , Seong Je Kyung , Jin Suk-Won , Kang Hyoung Jin , Kim Hyeon Ho , Choi Murim 
Published: December 2018

AbstractShwachman-Diamond syndrome (SDS; OMIM: #260400) is caused by variants inSBDS(Shwachman-Bodian-Diamond syndrome gene), which encodes a protein that plays an important role in ribosome assembly. Recent reports suggest that recessive variants inEFL1are also responsible for SDS. However, the precise genetic mechanism that leads toEFL1-induced SDS remains incompletely understood. Here we present three unrelated Korean SDS patients that carry biallelic pathogenic variants inEFL1with biased allele frequencies, resulting from a bone marrow-specific somatic uniparental disomy (UPD) in chromosome 15. The recombination events generated cells that were homozygous for the relatively milder variant, allowing for the evasion of catastrophic physiological consequences. Still, the milderEFL1variant was solely able to impair 80S ribosome assembly and induce SDS features in cell line, zebrafish, and mouse models. The loss ofEFL1resulted in a pronounced inhibition of terminal oligo-pyrimidine element-containing ribosomal protein transcript 80S assembly. Therefore, we propose a more accurate pathogenesis mechanism of EFL1 dysfunction that eventually leads to aberrant translational control and ribosomopathy.
Plant-Necrotroph Co-transcriptome Networks Illuminate a Metabolic Battlefield
Authors: Zhang Wei , Corwin Jason A. , Copeland Daniel , Feusier Julie , Eshbaugh Robert , Cook David E. , Atwell Susana , Kliebenstein Daniel J. 
Published: December 2018

AbstractA central goal of studying host-pathogen interaction research is to understand how the host and pathogen manipulate each other to promote their own fitness in a pathosystem. Co-transcriptomic approaches can simultaneously analyze dual transcriptomes during infection and provide a systematic map of the cross-kingdom communication between two species. Here we used the Arabidopsis-B. cinerea pathosystem to test how plant host and fungal pathogen interaction at the transcriptomic level during infection. We assessed the impact of natural genetic diversity in the pathogen and plant host by utilization of a collection of 96 isolates of B. cinerea infection on Arabidopsis wild-type and two mutants with jasmonate or salicylic acid compromised immunities. We identified ten B. cinerea gene co-expression networks (GCNs) that encode known or novel virulence mechanisms. We constructed a dual interaction network by combining four host-and ten pathogen-GCNs into a single network, which revealed potential connections between the fungal and plant GCNs involving both novel and conserved mechanisms. These co-transcriptome data shed lights on the potential mechanisms underlying host-pathogen interaction and illustrate the continued need for advancements of in planta analysis of dual-species dynamics.
Meiotic Drive and Survival Probability of Newly Inverted Chromosomes
Authors: Koury Spencer A. 
Published: December 2018

AbstractWhen a new gene arrangement is generated by spontaneous mutation its survival is uncertain and largely unaffected by associated fitness effects. However, if a new chromosomal inversion is introduced into a population already polymorphic for inversions, then its survival probability will be a function of the relative size, position, and linkage phase of the gene rearrangements. This dependence on structural features is due to the complex meiotic behavior of overlapping inversions generating asymmetric dyads, which in turn cause both underdominance and meiotic drive/drag. Therefore, survival probabilities of new inversions can be expressed in terms of the probability of forming an asymmetric dyad via crossing over in meiosis I and the probability of recovery from that asymmetric dyad during disjunction in meiosis II. This model of female meiotic drive was parameterized with data from published experiments on laboratory constructs inDrosophila melanogaster. Generalizing this analysis to all possible inversions predicts a bias towards larger, proximally located inversions having a shorter persistence time in populations. These population genetic predictions are consistent with cytological evidence from natural populations ofD. melanogaster. This research mathematically formalizes a cytogenetic mechanism for female meiotic drive/drag as the major force governing behavior of new gene arrangements entering populations, and therefore determining the genomic distribution of segregating inversion polymorphism.
Corrosion fatigue behavior of Al-5Mg coated AISI 316L stainless steel in sodium chloride environments under bending load
Authors: Öcal Mustafa , Sadeler Recep 
Published: December 2018

Purpose
The purpose of this study is to describe the environmentally assisted cracking (EAC) of AISI 316L stainless steel as bare and coated cases in several corrosion environments. The main purpose of this study is to extend the lifespan of 316L material under corrosive fatigue in sodium chloride environments.

Design/methodology/approach
Fatigue tests carried out by using a Schenk type plane bending fatigue machine made by Tokyokoki Co. A scanning electron microscope (SEM) was used to observe the fracture surfaces and tested specimen surfaces. The micro-Vickers hardness of specimens was measured by using a PC-controlled Buehler–Omnimet tester.

Findings
Under reciprocating bending condition (R = −1) the behavior of 316L SS bare samples and 316L SS coated with Al-5%Mg samples were investigated comparatively at room temperature in ambient air and in several corrosion solutions. The results obtained from the data showed that Al-5Mg coating procedure significantly stabilized the 316L SS even in the most aggressive environment 5 per cent NaCl solution as compared with bare samples.

Originality/value
Al-5Mg coating showed a stable structure under the corrosion liquids used in the experiments. The coating material served as a stable barrier between the base material and the corrosion fluid, thus ensuring a tightness even in long-term tests below the endurance limit.
Organizational culture: does it influence the way a company deals with CSR?
Authors: Castro-González Sandra , Bande Belén 
Published: December 2018

PurposeThis paper aims to show how managers model culture for employees to promote corporate social responsibility (CSR) practices.Design/methodology/approachThe proposal begins by highlighting the importance of culture for CSR implementation and then explains the impact of culture on employees.FindingsCurrently, many companies carry out activities under the CSR umbrella, but they may do so without considering whether their company’s context is ideal for such initiatives. They may also not consider the types of employees composing the workforce or their disposition toward CSR. In these circumstances, organizations may miss out on the added value of CSR practices.Originality/valueThe important influence of CSR practices on employees’ attitudes and behaviors at work requires organizations to implement these types of actions. Therefore, the contextual factors that are most likely to aid in developing and maintaining these practices must be acknowledged. This paper seeks to offer a new perspective to managers and human resource managers regarding the implementation of CSR activities.