bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diurnal rhythm of melatonin in young calves and intake of melatonin in milk
Authors: Valtonen M. , Kangas A.-P. , Voutilainen M. , Eriksson L. 
Published: May 2019

AbstractPeripheral concentrations of melatonin in the neonatal mammal are low and the diurnal rhythm is not apparent during the first days or weeks of life. Maternal milk provides a rhythmic although low source of melatonin to the newborn. Diurnal rhythm of serum melatonin was monitored in Friesian calves from birth to 4 weeks of age. Some of the calves under 1 week of age had no clear nocturnal elevation of serum concentrations of melatonin. Thereafter the nocturnal melatonin elevation was apparent in all calves and of the same amplitude as in adult cattle. The effect of oral intake of night-time milk or milk supplemented with low doses of synthetic melatonin was studied during day-time in a group of Ayrshire calves aged 1 to 5 weeks. Two and a half or 3 litres of night-time milk with a mean melatonin concentration of 40 pg/ml, providing 01 to 012 μg melatonin, did not cause any change in the day-time serum concentrations of melatonin, neither did addition of melatonin in doses up to 10 μg per animal. Only the highest dose of melatonin 200 μg in 2 l of milk (01 μg/ml) caused an elevation of day-time serum concentrations of melatonin. These studies show that the pineal gland of a very youg calf is functional and that the concentration of melatonin in blood does not reflect the concentration in milk at the time of feeding. The presence of melatonin in milk suggests, however, its potential significance for the neonate.
Analysis of cell population PDE models with general maturation rates
Authors: Liu Xinzhi , Sivaloganathan S. , Zhang Shenghai 
Published: May 2019

AbstractThis paper considers a cell population model with a general maturation rate. This model is described by a nonlinear PDE. We use the theory of operator semigroups to stud the problem under simple hypotheses on the growth function and the nonlinear term. By showing that a related operator generates a strongly continuous semigroup, we prove the existence of a classical solution of the nonlinear problem and its positivity. It is also proved that under simple hypotheses, the problem generates a semiflow. The invariance of the semiflow is studied as well.
Topological duality in humanoid robot dynamics
Authors: Ivancevic V. , Pearce C. E. M. 
Published: May 2019

AbstractA humanoid robot system may be viewed as a collection of segments coupled at rotational joints which geometrically represent constrained rotational Lie groups. This allows a study of the dynamics of the motion of a humanoid robot. Several formulations are possible. In this paper, dual invariant topological structures are constructed and analyzed on the finite-dimensional manifolds associated with the humanoid motion. Both cohomology and homology structures are examined on the tangent (Lagrangian) as well as on the cotangent (Hamiltonian) bundles on the manifold of the humanoid motion configuration. represented by the toral Lie group. It is established all four topological structures give in essence the same description of humanoid dynamics. Practically this means that whichever of these approaches we use, ultimately we obtain the same mathematical results.
A simple model for oilspill containment
Authors: Belward Shaun R. , Forbes Lawrence K. 
Published: May 2019

AbstractThis paper develops a simple model for the containment of oil behind a boom in water. The flow of water beneath the oil is assumed two dimensional (horizontal and vertical) and perpendicular to the boom. We look for steady solutions and assume the oil is so viscous that the fluid velocity within the oil is zero. We are able to calculate what shape the oilslick will form and under which circumstances the boom will be successful (that is, no oil escapes under the boom) based on the predicted depth of the slick at the boom.
Predictors for reoperation after lower limb amputation in patients with peripheral arterial disease
Authors: Berli Martin C. , Wanivenhaus Florian , Kabelitz Method , Götschi Tobias , Böni Thomas , Rancic Zoran , Waibel Felix W. A. 
Published: May 2019

Summary. Background: Major amputations in patients with peripheral arterial disease (PAD) carry a high risk for complications, including revision of the amputation, sometimes to a higher level. Determining a safe level for amputation with good wound healing potential depends largely on vascular measurements. This study evaluated potential predictive factors for revision of major lower extremity amputations in patients with PAD. Patients and methods: A retrospective chart review of all major lower extremity amputations at our institution was conducted. Amputations due to trauma or tumor and below-ankle amputations were excluded. Patient demographics, level/type of amputation, level/time of revision, comorbidities and risk factors were extracted. Results: 180 patients with PAD, mean age 66.48 (range: 31–93) years, 125 (69.4%) male were included. Most (154/180, 86.6%) underwent below-knee amputation. 71 (39.4%) patients had coronary arterial disease, 104 (57.8%) had diabetes. More than half of patients, (93/138; 51.7%) had undergone previous balloon angioplasty. 44 (30%) patients required revision surgery: 42/180 (23.3%) were revised at the same level, and in 12/180 (6.7%) a more proximal amputation was necessary. PAD stage was not associated with the level of reamputation (p = 0.4369). Significantly more patients who had previous balloon angioplasty required revision surgery (66.7% versus 45.2%, p = 0.009). 67 (37.2%) patients underwent preoperative TcPO2 measurement: 40/67 (59.7%) had TcPO2 ≥ 40 mmHg; 4/67 (6%) had TcPO2 < 10 mmHG. Three patients with TcPO2 ≥ 40 mmHg, one with 30 mmHg ≤ TcPO2 ≤ 40 mmHg and one with 10 mmHg ≤ TcPO2 ≤ 20 mmHg required re-amputation to a more proximal level. Conclusions: TcPO2 measurements are useful for determining level of lower limb amputation and predicting wound healing problems when an amputation level with TcPO2 < 40 mmHg is chosen. In transtibial amputations, TcPO2 ≥ 40 mmHg does not safely predict wound healing.
Attachment Style Predicts Cortical Activity in Temporoparietal Junction (TPJ)
Authors: Baskak Bora , Kır Yagmur , Sedes Nilay , Kuşman Adnan , Türk Eylem Gökce , Baran Zeynel , Gönüllü Ipek , Artar Müge , Munir Kerim 
Published: May 2019

Abstract. Results of the behavioral studies suggest that attachment styles may have an enduring effect upon theory of mind (ToM). However biological underpinnings of this relationship are unclear. Here, we compared securely and insecurely attached first grade university students ( N = 56) in terms of cortical activity measured by 52 channel Functional Near Infrared Spectroscopy (fNIRS) during the Reading the Mind from the Eyes Test (RMET). The control condition involved gender identification via the same stimuli. We found that the ToM condition evoked higher activity than the control condition particularly in the right hemisphere. We observed higher activity during the ToM condition relative to the control condition in the secure group (SG), whereas the overall cortical activity evoked by the two conditions was indistinguishable in the insecure group (ISG). Higher activity was observed in channels corresponding to right superior temporal and adjacent parietal cortices in the SG relative to the ISG during the ToM condition. Dismissive attachment scores were negatively correlated with activity in channels that correspond to right superior temporal cortex. These results suggest that attachment styles do have an effect on representation of ToM in terms of cortical activity in late adolescence. Particularly, dismissive attachment is represented by lower activity in the right superior temporal cortex during ToM, which might be related to weaker social need and habitual unwillingness for closeness among this group of adolescents.
Improving Surface Current Estimation From Geostationary Ocean Color Imager Using Tidal Ellipse and Angular Limitation
Authors: Chen Jian , Chen Jianyu , Cao Zhengyi , Shen Yu 
Published: May 2019

AbstractSea surface currents (SSCs) in coastal regions can be mapped from successive optical images observed by satellite. We implemented the maximum cross‐correlation (MCC) algorithm to Geostationary Ocean Color Imager‐derived total suspended matter in the East China Sea in this study. We developed a new data filtering method to improve the accuracy of SSC vector sequence using estimated tidal ellipse. The proposed method eliminated spurious vectors by considering direction order and suitable angular limitation. The obtained average velocity of SSC reached 0.72 m/s, which was close to the buoy‐measured average speed of 0.64 m/s. Compared with existing spurious vector eliminating method, the average angular error was improved significantly by 28–38% and the average relative magnitude error was improved by 3% in the case study.
Direct Measurements of Upper Ocean Horizontal Velocity and Vertical Shear in the Tropical North Atlantic at 4°Ν, 23°W
Authors: Perez Renellys C. , Foltz Gregory R. , Lumpkin Rick , Schmid Claudia 
Published: May 2019

AbstractThe Tropical Atlantic Current Observations Study measured upper ocean horizontal velocity from a mooring at 4°N, 23°W, at discrete depths between 7 and 87 m, in order to observe the temporal and vertical structure of the currents. Between March 2017 and March 2018, mean zonal velocity and vertical shear were strongest between 32 and 37 m. Near‐surface mean eastward currents during this period were weaker than the long‐term mean but within the range of previously observed values given the high interannual variability. Interannual variability of the zonal velocity exceeded that of the meridional velocity, was primarily geostrophically driven, and was strongly influenced by the large‐scale currents. Energetic tropical instability waves (TIWs) were observed in early summer and late fall of 2017. Meridional velocity fluctuations associated with the TIWs were far larger than those of zonal velocity and extended down to 87 m. These fluctuations propagated upward to the surface with vertical phase speeds between 12 and 15 m/day. Coherent velocity and vertical shear variations emerged in a TIW composite. However, the vertical shears observed during the TIW season were modest compared to the large shear measured in spring 2017 and winter 2018. As a result, TIWs may be less crucial for vertical turbulent cooling at 4°N, 23°W than they are near the equator. To observe the intense winter/spring vertical shear events, fine vertical (less than 10 m) and diurnal temporal sampling is needed.
Fast lithium growth and short circuit induced by localized-temperature hotspots in lithium batteries
Authors: Zhu Yangying , Xie Jin , Pei Allen , Liu Bofei , Wu Yecun , Lin Dingchang , Li Jun , Wang Hansen , Chen Hao , Xu Jinwei , Yang Ankun , Wu Chun-Lan , Wang Hongxia , Chen Wei , Cui Yi 
Published: May 2019

AbstractFast-charging and high-energy-density batteries pose significant safety concerns due to high rates of heat generation. Understanding how localized high temperatures affect the battery is critical but remains challenging, mainly due to the difficulty of probing battery internal temperature with high spatial resolution. Here we introduce a method to induce and sense localized high temperature inside a lithium battery using micro-Raman spectroscopy. We discover that temperature hotspots can induce significant lithium metal growth as compared to the surrounding lower temperature area due to the locally enhanced surface exchange current density. More importantly, localized high temperature can be one of the factors to cause battery internal shorting, which further elevates the temperature and increases the risk of thermal runaway. This work provides important insights on the effects of heterogeneous temperatures within batteries and aids the development of safer batteries, thermal management schemes, and diagnostic tools.
Low potential enzymatic hydride transfer via highly cooperative and inversely functionalized flavin cofactors
Authors: Willistein Max , Bechtel Dominique F. , Müller Christina S. , Demmer Ulrike , Heimann Larissa , Kayastha Kanwal , Schünemann Volker , Pierik Antonio J. , Ullmann G. Matthias , Ermler Ulrich , Boll Matthias 
Published: May 2019

AbstractHydride transfers play a crucial role in a multitude of biological redox reactions and are mediated by flavin, deazaflavin or nicotinamide adenine dinucleotide cofactors at standard redox potentials ranging from 0 to –340 mV. 2-Naphthoyl-CoA reductase, a key enzyme of oxygen-independent bacterial naphthalene degradation, uses a low-potential one-electron donor for the two-electron dearomatization of its substrate below the redox limit of known biological hydride transfer processes at E°’ = −493 mV. Here we demonstrate by X-ray structural analyses, QM/MM computational studies, and multiple spectroscopy/activity based titrations that highly cooperative electron transfer (n = 3) from a low-potential one-electron (FAD) to a two-electron (FMN) transferring flavin cofactor is the key to overcome the resonance stabilized aromatic system by hydride transfer in a highly hydrophobic pocket. The results evidence how the protein environment inversely functionalizes two flavins to switch from low-potential one-electron to hydride transfer at the thermodynamic limit of flavin redox chemistry.
The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription
Authors: Malik Navdeep , Yan Hualong , Moshkovich Nellie , Palangat Murali , Yang Howard , Sanchez Vanesa , Cai Zhuo , Peat Tyler J. , Jiang Shunlin , Liu Chengyu , Lee Maxwell , Mock Beverly A. , Yuspa Stuart H. , Larson Daniel , Wakefield Lalage M. , Huang Jing 
Published: May 2019

AbstractTranslation and transcription are frequently dysregulated in cancer. These two processes are generally regulated by distinct sets of factors. The CBFB gene, which encodes a transcription factor, has recently emerged as a highly mutated driver in a variety of human cancers including breast cancer. Here we report a noncanonical role of CBFB in translation regulation. RNA immunoprecipitation followed by deep sequencing (RIP-seq) reveals that cytoplasmic CBFB binds to hundreds of transcripts and regulates their translation. CBFB binds to mRNAs via hnRNPK and enhances translation through eIF4B, a general translation initiation factor. Interestingly, the RUNX1 mRNA, which encodes the transcriptional partner of CBFB, is bound and translationally regulated by CBFB. Furthermore, nuclear CBFB/RUNX1 complex transcriptionally represses the oncogenic NOTCH signaling pathway in breast cancer. Thus, our data reveal an unexpected function of CBFB in translation regulation and propose that breast cancer cells evade translation and transcription surveillance simultaneously through downregulating CBFB.
Room-Temperature Sputtered SnO2 as Robust Electron Transport Layer for Air-Stable and Efficient Perovskite Solar Cells on Rigid and Flexible Substrates
Authors: Kam Matthew , Zhang Qianpeng , Zhang Daquan , Fan Zhiyong 
Published: May 2019

AbstractExtraordinary photovoltaic performance and intriguing optoelectronic properties of perovskite solar cells (PSCs) have aroused enormous interest from both academic research and photovoltaic (PV) industry. In order to bring PSC technology from laboratory to market, material stability, device flexibility, and scalability are important issues to address for vast production. Nevertheless, PSCs are still primarily prepared by solution methods which limit film scalability, while high-temperature processing of metal oxide electron transport layer (ETL) makes PSCs costly and incompatible with flexible substrates. Here, we demonstrate rarely-reported room-temperature radio frequency (RF) sputtered SnO2 as a promising ETL with suitable band structure, high transmittance, and excellent stability to replace its solution-processed counterpart. Power conversion efficiencies (PCEs) of 12.82% and 5.88% have been achieved on rigid glass substrate and flexible PEN substrate respectively. The former device retained 93% of its initial PCE after 192-hour exposure in dry air while the latter device maintained over 90% of its initial PCE after 100 consecutive bending cycles. The result is a solid stepping stone toward future PSC all-vapor-deposition fabrication which is being widely used in the PV industry now.
Interleukin-7 Contributes to the Invasiveness of Prostate Cancer Cells by Promoting Epithelial–Mesenchymal Transition
Authors: Seol Min A. , Kim Jin-Hee , Oh Keunhee , Kim Gwanghun , Seo Myung Won , Shin Young-Kyoung , Sim Ji Hyun , Shin Hyun Mu , Seo Bo Yeon , Lee Dong-Sup , Ku Ja-Lok , Han Ilkyu , Kang Insoo , Park Serk In , Kim Hang-Rae 
Published: May 2019

AbstractPrecise mechanisms underlying interleukin-7 (IL-7)-mediated tumor invasion remain unclear. Thus, we investigated the role of IL-7 in tumor invasiveness using metastatic prostate cancer PC-3 cell line derivatives, and assessed the potential of IL-7 as a clinical target using a Janus kinase (JAK) inhibitor and an IL-7-blocking antibody. We found that IL-7 stimulated wound-healing migration and invasion of PC-3 cells, increased phosphorylation of signal transducer and activator of transcription 5, Akt, and extracellular signal-regulated kinase. On the other hand, a JAK inhibitor and an IL-7-blocking antibody decreased the invasiveness of PC-3 cells. IL-7 increased tumor sphere formation and expression of epithelial–mesenchymal transition (EMT) markers. Importantly, lentiviral delivery of IL-7Rα to PC-3 cells significantly increased bone metastasis in an experimental murine metastasis model compared to controls. The gene expression profile of human prostate cancer cells from The Cancer Genome Atlas revealed that EMT pathways are strongly associated with prostate cancers that highly express both IL-7 and IL-7Rα. Collectively, these data suggest that IL-7 and/or IL-7Rα are promising targets of inhibiting tumor metastasis.
Distribution of Functional CD4 and CD8 T cell Subsets in Blood and Rectal Mucosal Tissues
Authors: Amancha Praveen Kumar , Ackerley Cassie G. , Duphare Chandni , Lee Mark , Hu Yi-Juan , Amara Rama R. , Kelley Colleen F. 
Published: May 2019

AbstractA better understanding of the distribution and functional capacity of CD4 T helper (Th) and CD8 T cytotoxic (Tc) cell subsets in the rectal mucosa (RM), a major site for HIV acquisition and replication, in adults is needed. In this study, we compared the distribution of Th and Tc cell subsets between blood and RM compartments in 62 HIV negative men, focusing primarily on IL-17-producing CD4 and CD8 T cells due to their importance in establishing and maintaining mucosal defenses, and examined associations between the frequencies of Th17 and Tc17 cell subsets and the availability of highly HIV-susceptible target cells in the RM. The RM exhibited a distinct immune cell composition comprised of higher frequencies of Th2, Th17, and Tc17 cells compared to the peripheral blood. The majority of Tc17 cells in RM were quadruple-cytokine producers (IL-17A+, IFN-γ+, TNF-α+, and IL4+), whereas most Th17 cells in blood and RM were single IL-17A producers or dual-cytokine producers (IL-17A+TNF-α+). In a separate cohort of 21 HIV positive men, we observed similar tissue distributions of Th and Tc cell subsets, although Tc17 cell frequencies in both blood and tissues were very low. Higher frequencies of multi-cytokine-producing Th17 and Tc17 cells in RM of HIV negative men positively correlated with increased mucosal HIV target cells, suggesting a need to further characterize the effector functions of these cells and their role in HIV acquisition and pathogenesis.
Construction and characterization of metal ion-containing DNA nanowires for synthetic biology and nanotechnology
Authors: Vecchioni Simon , Capece Mark C. , Toomey Emily , Nguyen Le , Ray Austin , Greenberg Alissa , Fujishima Kosuke , Urbina Jesica , Paulino-Lima Ivan G. , Pinheiro Vitor , Shih Joseph , Wessel Gary , Wind Shalom J. , Rothschild Lynn 
Published: May 2019

AbstractDNA is an attractive candidate for integration into nanoelectronics as a biological nanowire due to its linear geometry, definable base sequence, easy, inexpensive and non-toxic replication and self-assembling properties. Recently we discovered that by intercalating Ag+in polycytosine-mismatch oligonucleotides, the resulting C-Ag+-C duplexes are able to conduct charge efficiently. To map the functionality and biostability of this system, we built and characterized internally-functionalized DNA nanowires through non-canonical, Ag+-mediated base pairing in duplexes containing cytosine-cytosine mismatches. We assessed the thermal and chemical stability of ion-coordinated duplexes in aqueous solutions and conclude that the C-Ag+-C bond forms DNA duplexes with replicable geometry, predictable thermodynamics, and tunable length. We demonstrated continuous ion chain formation in oligonucleotides of 11–50 nucleotides (nt), and enzyme ligation of mixed strands up to six times that length. This construction is feasible without detectable silver nanocluster contaminants. Functional gene parts for the synthesis of DNA- and RNA-based, C-Ag+-C duplexes in a cell-free system have been constructed in anEscherichia coliexpression plasmid and added to the open-source BioBrick Registry, paving the way to realizing the promise of inexpensive industrial production. With appropriate design constraints, this conductive variant of DNA demonstrates promise for use in synthetic biological constructs as a dynamic nucleic acid component and contributes molecular electronic functionality to DNA that is not already found in nature. We propose a viable route to fabricating stable DNA nanowires in cell-free and synthetic biological systems for the production of self-assembling nanoelectronic architectures.
A highly flexible and repeatable genotyping method for aquaculture studies based on target amplicon sequencing using next-generation sequencing technology
Authors: Sato Mana , Hosoya Sho , Yoshikawa Sota , Ohki Shun , Kobayashi Yuki , Itou Takuya , Kikuchi Kiyoshi 
Published: May 2019

AbstractStudies using genome-wide single nucleotide polymorphisms (SNPs) have become commonplace in genetics and genomics, due to advances in high-throughput sequencing technologies. Since the numbers of required SNPs and samples vary depending on each research goal, genotyping technologies with high flexibility in the number of SNPs/samples and high repeatability have been intensively investigated. For example, the ultrahigh-multiplexed amplicon sequencing, Ion AmpliSeq, has been used as a high-throughput genotyping method mainly for diagnostic purposes. Here, we designed a custom panel targeting 3,187 genome-wide SNPs of fugu,Takifugu rubripes, and applied it for genotyping farmed fugu to test its feasibility in aquaculture studies. We sequenced two libraries consisting of different pools of individuals (n = 326 each) on the Illumina MiSeq sequencer. Consequently, over 99% target regions (3,178 SNPs) were amplified and 2,655 SNPs were available after filtering steps. Strong correlation was observed in the mean depth of coverage of each SNP between duplicate runs (r = 0.993). Genetic analysis using these genotype data successfully detected the known population structure and the sex determining locus of fugu. These results show the method is superior in repeatability and flexibility, and suits genetic studies including molecular breeding, such as marker assisted and genomic selection.