bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dual recognition of H3K4me3 and H3K27me3 by a plant histone reader SHL
Authors: Qian Shuiming , Lv Xinchen , Scheid Ray N. , Lu Li , Yang Zhenlin , Chen Wei , Liu Rui , Boersma Melissa D. , Denu John M. , Zhong Xuehua , Du Jiamu 
Published: June 2018

AbstractThe ability of a cell to dynamically switch its chromatin between different functional states constitutes a key mechanism regulating gene expression. Histone mark “readers” display distinct binding specificity to different histone modifications and play critical roles in regulating chromatin states. Here, we show a plant-specific histone reader SHORT LIFE (SHL) capable of recognizing both H3K27me3 and H3K4me3 via its bromo-adjacent homology (BAH) and plant homeodomain (PHD) domains, respectively. Detailed biochemical and structural studies suggest a binding mechanism that is mutually exclusive for either H3K4me3 or H3K27me3. Furthermore, we show a genome-wide co-localization of SHL with H3K27me3 and H3K4me3, and that BAH-H3K27me3 and PHD-H3K4me3 interactions are important for SHL-mediated floral repression. Together, our study establishes BAH-PHD cassette as a dual histone methyl-lysine binding module that is distinct from others in recognizing both active and repressive histone marks.
Lateralized hippocampal oscillations underlie distinct aspects of human spatial memory and navigation
Authors: Miller Jonathan , Watrous Andrew J. , Tsitsiklis Melina , Lee Sang Ah , Sheth Sameer A. , Schevon Catherine A. , Smith Elliot H. , Sperling Michael R. , Sharan Ashwini , Asadi-Pooya Ali Akbar , Worrell Gregory A. , Meisenhelter Stephen , Inman Cory S. , Davis Kathryn A. , Lega Bradley , Wanda Paul A. , Das Sandhitsu R. , Stein Joel M. , Gorniak Richard , Jacobs Joshua 
Published: June 2018

AbstractThe hippocampus plays a vital role in various aspects of cognition including both memory and spatial navigation. To understand electrophysiologically how the hippocampus supports these processes, we recorded intracranial electroencephalographic activity from 46 neurosurgical patients as they performed a spatial memory task. We measure signals from multiple brain regions, including both left and right hippocampi, and we use spectral analysis to identify oscillatory patterns related to memory encoding and navigation. We show that in the left but not right hippocampus, the amplitude of oscillations in the 1–3-Hz “low theta” band increases when viewing subsequently remembered object–location pairs. In contrast, in the right but not left hippocampus, low-theta activity increases during periods of navigation. The frequencies of these hippocampal signals are slower than task-related signals in the neocortex. These results suggest that the human brain includes multiple lateralized oscillatory networks that support different aspects of cognition.
Metabolomic profiles of bovine cumulus cells and cumulus-oocyte-complex-conditioned medium during maturation in vitro
Authors: Uhde Karen , van Tol Helena T. A. , Stout Tom A. E. , Roelen Bernard A. J. 
Published: June 2018

AbstractCumulus cells are essential for nutrition of oocytes during maturation. In the absence of cumulus cells during maturation, oocyte developmental competence is severely compromised. In this study, we matured bovine cumulus-oocyte-complexes (COCs) for 8 h, the cumulus cells were removed and denuded oocytes were further matured for 15 h in either the medium conditioned by the initial 8 h culture, or in fresh unconditioned medium. Denuded oocytes that completed maturation in COC-conditioned medium demonstrated better developmental potential than denuded oocytes that completed maturation in standard maturation medium. An inventory was made of the metabolites secreted by COCs into the maturation medium during the first 8 h, from 8 to 23 h, and during an entire 23 h maturation protocol; the metabolomic changes in the cumulus cells during maturation were also investigated. In maturation medium, 173 biochemical components were detected compared to 369 different metabolites in cumulus cells. Significant changes in metabolomic components were evident in maturation medium and in cumulus cells during maturation, with most of the changes related to amino acid, carbohydrate, and lipid metabolism. The importance of two detected biochemicals, creatine and carnitine, for oocyte maturation was further investigated. The presence of carnitine, but not creatine during oocyte in vitro maturation improved the developmental competence of denuded oocytes.
Nanoscratching technique for highly oriented liquid crystal materials
Authors: Suh Ahram , Yoon Dong Ki 
Published: June 2018

AbstractA simple, fast, and cost-effective technique to obtain highly oriented thermotropic and lyotropic liquid crystal (LC) phases using a simple nanoscratching method is presented. Highly aligned linear nanogrooves are fabricated by scratching substrates such as normal, indium tin oxide (ITO), curved glasses, and ITO-coated polyethylene terephthalate (PET) film using diamond lapping films. To demonstrate the feasibility of the platform, typical thermotropic and lyotropic LC materials in the nematic phase are used to demonstrate the well-aligned domains along with the resulting scratched nanogrooves. The polarised optical microscopy (POM) images show excellent dark and bright states depending on the sample rotation, proving that the LC molecules are well aligned. The electro-optical performance of the twisted nematic (TN) mode LC display fabricated using the nanogrooves is also measured and indicates reliable results compared with that of the conventional device. Indeed, scratch-induced nanogrooves are well generated on the curved substrate and ITO-coated PET film to show versatility of our technique. Our platform can suggest a new nanofabrication way to make various electro-optical devices as well as other patterning applications.
UWB circularly polarised slot antenna with modified ground plane and L‐shaped radiator
Authors: Xu Rui , Li Jianying , Liu Jie , Zhou Shi Gang , Wei Kun 
Published: June 2018

An UWB circularly polarised (CP) slot antenna with simple structure is presented in this Letter. By etching a simple C‐shaped slot on the planar ground, adopting an L‐shaped strip as a radiator, and employing an off‐centre‐fed structure, the proposed CP antenna can easily be realised with both wideband impedance bandwidth (IBW) and 3 dB axial‐ratio bandwidth (ARBW). The impedance and ARBWs of this antenna can be further expanded by adding a matching stub and cutting an L‐shaped slit on the lower ground. This antenna can realise a wideband IBW (S11 < −10 dB) of about 3.62 GHz (124.4%, 1.10–4.72 GHz) and 3 dB ARBW of about 3.6 GHz (115.2%, 1.26–4.68 GHz).
High frequency impulse ground penetrating radar application in assessment of interlayer connections
Authors: Sudyka Jacek , Krysiński Lech , Zofka Adam , Pszczoła Marek , Jaskuła Piotr 
Published: June 2018

Ground Penetrating Radar (GPR) technique is commonly used in the nondestructive evaluation of pavement structures. In particular, this method is used to estimate thicknesses of pavement layers as well as it can be utilized in advanced studies of pavement structures. The device presented in this paper comprise the high frequency impulse antennas that allow for investigating the interlayer zones in terms of their electromagnetic properties (e.g. dielectric constant). In some cases these electromagnetic responses can be suitable in the assessment of layer bonding in the pavement structure. This paper discusses the assessment of the quality of asphalt pavement interlayer bonding with the use of high frequency GPR techniques. The preliminary laboratory measurements were performed using an impulse antenna in the zero-offset configuration combined with the large-scale models simulating an idealized horizontal delamination. These measurements allowed to estimate the antenna sensitivity to detect interlayer connection under dry and wet conditions. Analysis of collected results led to formulating practical conclusions regarding critical limitations of the measuring system and adequate methods of signal processing and interpretation.
The field investigations consisted of the GPR measurements along selected road sections and collection of the core samples at the locations associated with the specific reflexes. Inspection of the cores provided some real insights into the structure of different delaminations associated with characteristic reflexes. Analysis showed the reflection properties are able to expresses some important features of the interlayer zone, such as delaminations, presence of alien material at the interface, insufficient compaction occurring at the base of layer, and water penetration.
The Effect of Additives on the Properties of Metakaolin and Fly Ash Based Geopolymers
Authors: Łach Michał , Korniejenko Kinga , Hebdowska-Krupa Maria , Mikuła Janusz 
Published: June 2018

The main motivation of research work is connected with environmental issues. The production of the most important building material of the 20th century - Portland cement technology is associated with significant environmental pollution. The process requires very high temperature and it is energy consuming. During the manufacturing also takes place emission of significant amounts of carbon dioxide and highly toxic nitrogen oxides into the atmosphere These factors show that new solution in this area is required. The most promising alternative is inorganic polymer (geopolymer) technology.
The main objective of the presented research work was to design a new composite for practical applications, especially in construction industry. The paper presents the results of research of geopolymer composites based on geopolymer binders made of metakaolin and fly ash with the addition of titanium oxide and aluminum-calcium cements (including mainly calcium monoglinate) in amount of 4 and 6% by weight. Research methods applied: tests for mechanical properties (compressive strength tests), scanning microscopy investigations (SEM) and X-Ray Diffraction (XRD).
The results show that the addition of aluminum-calcium cements (including calcium monoglinate) significantly increases the compressive strength of geopolymers. Geopolymers based on fly ash with the addition of 6% calcium-aluminum cement with a calcium monoglinate content above 69% are characterized by compressive strength above 50 MPa, while geopolymers from metakaolin with the same additive were characterized by compressive strength above 80 MPa.
Hearing Aids as a Treatment for Tinnitus Patients with Slight to Mild Sensorineural Hearing Loss
Authors: Johnson Carole , Baldwin Johnathan , Barton Kristen , Mathews Caitlyn , Danhauer Jeffrey , Kimball Suzanne 
Published: June 2018

AbstractPersons with clinically significant tinnitus also may have mild sensorineural hearing loss (MSNHL). The purpose of this study was to describe patients with tinnitus and MSNHL and factors predicting hearing-aid uptake (HAU). We conducted a retrospective chart review with regression modeling of patients presenting to a specialty tinnitus clinic over a 2.5-year period. Stepwise logistic regression on data from patient charts was conducted. Of 133 patients seen, two-thirds had MSNHL (95% confidence interval [CI]: 58.9–75.0; mean age = 53.4 years; standard deviation = 14.5); approximately 50% had severe-to-catastrophic tinnitus. Logistic regression indicated that four-frequency pure-tone average (FFPTA; left) (β = 0.3899, χ
2 = 10.96, degrees of freedom [DF] = 1, p = 0.0009) and age (β = 0.1273, χ
2 = 4.86, DF = 1, p = 0.0274) were positively associated with HAU; tinnitus severity was inversely related (β = − 1.0533, χ
2 = 4.24, DF = 1, p = 0.0395). Adjusting for key variables, odds of receiving hearing aids was 1.14 (95% CI: 1.01–1.27) times higher with every year increase in age, 1.48 (95% CI: 1.17–1.86) times higher per one point increase in FFPTA (left), and 0.35 (95% CI: 0.13–0.95) times less per one point increase in tinnitus severity score. Reasons why HAU was not high for this special sample of young adults with severe tinnitus and MSNHL are discussed; hearing aid treatment requires extensive counseling and follow-up for this population.
Regional Tissue Oxygen Extraction and Severity of Anemia in Very Low Birth Weight Neonates: A Pilot NIRS Analysis
Authors: Mintzer Jonathan , Parvez Boriana , La Gamma Edmund 
Published: June 2018

Objective Anemia causes blood flow redistribution and altered tissue metabolic behavior to sustain homeostatic oxygen consumption. We hypothesized that anemia severity would correlate with increased regional fractional tissue oxygen extraction among premature neonates.
Study Design Regional oxygen extraction was calculated using pulse oximetry and near-infrared spectroscopy data among neonates <1,250 g during their first 10 postnatal days. Oxygen extraction was assessed for correlations with raw hematocrit levels and following grouping into hematocrit quartiles.
Results Twenty-seven neonates with gestational age 27 ± 2 weeks and birth weight 966 ± 181 g underwent 116 hematocrit determinations. Cerebral and flank oxygen extraction inversely correlated with hematocrit (cerebral r = −0.527, p = 0.005; flank r = −0.485, p = 0.01). Increased cerebral oxygen extraction was observed for the lowest three hematocrit quartiles (Q1 0.26 ± 0.08, p = 0.004; Q2 0.24 ± 0.09, p = 0.01; Q3 0.25 ± 0.09, p = 0.03; all compared with Q4 0.18 ± 0.10). Increased flank oxygen extraction occurred for the lowest two quartiles (Q1 0.36 ± 0.12, p < 0.001; Q2 0.35 ± 0.11, p < 0.001; compared with Q4 0.22 ± 0.13). Splanchnic oxygen extraction demonstrated no similar correlations.
Conclusion Increases in tissue oxygen extraction may indicate early pathophysiologic responses to nascent anemia in premature neonates.
National Survey of Neonatal Intensive Care Unit Medication Safety Practices
Authors: Greenberg Rachel , Smith P. , Bose Carl , Clark Reese , Cotten C. , DeRienzo Chris 
Published: June 2018

Objective We conducted a detailed survey to identify medication safety practices among a large network of United States neonatal intensive care units (NICUs).
Methods We created a 53-question survey to assess 300 U.S. NICU's demographics, medication safety practices, adverse drug event (ADE) reporting, and ADE response plans.
Results Among the 164 (55%) NICUs that responded to the survey, more than 85% adhered to practices including use of electronic health records, computerized physician order entry, and clinical decision support; fewer reported adopting barcoding, formal safety surveys, and formal culture training; 137 of 164 (84%) developed at least one NICU-specific order-set with a median of 10 order-sets.
Conclusion Among our survey of 164 NICUs, we found that many safety practices remain unused. Understanding safety practice variation is critical to prevent ADEs and other negative infant outcomes. Future efforts should focus on linking safety practices identified from our survey with ADEs and infant outcomes.
Pilgrimage, religious tourism and heritage in Calabria.
Authors: Bellio A. 
Published: June 2018

Abstract

This chapter analyses the contemporary forms of pilgrimage and religious tourism in Calabria from a specific perspective, that of mystic figures in present times. Focus is placed on Fratel Cosimo (i.e. Brother Cosimo), a contemporary figure at the origin of a very important cult in Calabria. Cosimo's experience gave rise to a community dedicated to the Madonna dello Scoglio (literally, Our Lady of the Rock) as well as to new facilities needed to host and manage the enormous flow of visitors to the pilgrimage site of Santa Domenica in Calabria.
Radiology
Authors: Cascarini Luke , Schilling Clare , Gurney Ben , Brennan Peter 
Published: June 2018

Abstract
This chapter discusses radiology in oral and maxillofacial surgery, including Guidelines for dental radiology, Dental radiography, Panoramic radiography, Ultrasonography, Computed Tomography (CT), Cone beam CT, Magnetic Resonance Imaging (MRI), Nuclear medicine imaging and Sialography
Essential skills
Authors: Cascarini Luke , Schilling Clare , Gurney Ben , Brennan Peter 
Published: June 2018

Abstract
This chapter discusses essential skills in oral and maxillofacial surgery, including Local anaesthesia, Intra-oral suturing, Managing the bleeding socket, Managing the dry socket, Suturing facial lacerations, Packing the nose and midface for bleeding, Re-implanting and splinting teeth, Temporomandibular joint relocation, Bridle wiring and intermaxillary fixation, Incision and drainage of intra-and-extra-oral abscesses, Biopsy of intra- and extra-oral lesions, Taking dental impressions, and Cricothyroidotomy
De novo lipogenesis represents a therapeutic target in mutant Kras non‐small cell lung cancer
Authors: Singh Anju , Ruiz Christian , Bhalla Kavita , Haley John A. , Li Qing Kay , Acquaah‐Mensah George , Montal Emily , Sudini Kuladeep R. , Skoulidis Ferdinandos , Wistuba Ignacio I. , Papadimitrakopoulou Vassiliki , Heymach John V. , Boros Laszlo G. , Gabrielson Edward , Carretero Julian , Wong Kwok‐Kin , Haley John D. , Biswal Shyam , Girnun Geoffrey D. 
Published: June 2018

ABSTRACTOncogenic Kras mutations are one of the most common alterations in non‐small cell lung cancer and are associated with poor response to treatment and reduced survival. Driver oncogenes, such as Kras are now appreciated for their ability to promote tumor growth via up‐regulation of anabolic pathways. Therefore, we wanted to identify metabolic vulnerabilities in Kras‐mutant lung cancer. Using the KrasLSL‐G12D lung cancer model, we show that mutant Kras drives a lipogenic gene‐expression program. Stable‐isotope analysis reveals that mutant Kras promotes de novo fatty acid synthesis in vitro and in vivo. The importance of fatty acid synthesis in Kras‐induced tumorigenesis was evident by decreased tumor formation in KrasLSL‐G12D mice after treatment with a fatty acid synthesis inhibitor. Importantly, with gain and loss of function models of mutant Kras, we demonstrate that mutant Kras potentiates the growth inhibitory effects of several fatty acid synthesis inhibitors. These studies highlight the potential to target mutant Kras tumors by taking advantage of the lipogenic phenotype induced by mutant Kras.—Singh, A., Ruiz, C., Bhalla, K., Haley, J. A., Li, Q. K., Acquaah‐Mensah, G., Montal, E., Sudini, K. R., Skoulidis, F., Wistuba, I. I., Papadimitrakopoulou, V., Heymach, J. V., Boros, L. G., Gabrielson, E., Carretero, J., Wong, K.‐K., Haley, J.D., Biswal, S., Girnun, G.D. De novo lipogenesis represents a therapeutic target in mutant Kras non‐small cell lung cancer. FASEB J. 32, 7018–7027 (2018). www.fasebj.org
The chemerin knockout rat reveals chemerin dependence in female, but not male, experimental hypertension
Authors: Watts Stephanie W. , Darios Emma S. , Mullick Adam E. , Garver Hannah , Saunders Thomas L. , Hughes Elizabeth D. , Filipiak Wanda E. , Zeidler Michael G. , McMullen Nichole , Sinal Christopher J. , Kumar Ramya K. , Ferland David J. , Fink Gregory D. 
Published: June 2018

ABSTRACTMeasures of the adipokine chemerin are elevated in multiple cardiovascular diseases, including hypertension, but little mechanistic work has been done to implicate chemerin as being causative in such diseases. The chemerin knockout (KO) rat was created to test the hypothesis that removal of chemerin would reduce pressure in the normal and hypertensive state. Western analyses confirmed loss of chemerin in the plasma and tissues of the KO vs. wild‐type (WT) rats. Chemerin concentration in plasma and tissues was lower in WT females than in WT males, as determined by Western analysis. Conscious male and female KO rats had modest differences in baseline measures vs. the WT that included systolic, diastolic, mean arterial and pulse pressures, and heart rate, all measured telemetrically. The mineralocorticoid deoxycorticosterone acetate (DOCA) and salt water, combined with uninephrectomy as a hypertensive stimulus, elevated mean and systolic blood pressures of the male KO higher than the male WT. By contrast, all pressures in the female KO were lower than their WT throughout DOCA‐salt treatment. These results revealed an unexpected sex difference in chemerin expression and the ability of chemerin to modify blood pressure in response to a hypertensive challenge.—Watts, S. W., Darios, E. S., Mullick, A. E., Garver, H., Saunders, T. L., Hughes, E. D., Filipiak, W. E., Zeidler, M. G., McMullen, N., Sinal, C. J., Kumar, R. K., Ferland, D. J., Fink, G. D. The chemerin knockout rat reveals chemerin dependence in female, but not male, experimental hypertension. FASEB J. 32, 6596–6614 (2018). www.fasebj.org
Oxidant sensor cation channel TRPM2 regulates neutrophil extracellular trap formation and protects against pneumoseptic bacterial infection
Authors: Tripathi Jitendra Kumar , Sharma Atul , Sukumaran Pramod , Sun Yuyang , Mishra Bibhuti Bhusan , Singh Brij Bhan , Sharma Jyotika 
Published: June 2018

ABSTRACTNeutrophil extracellular trap (NET) formation constitutes an important extracellular antimicrobial function of neutrophils that plays a protective role in bacterial pneumonia. Formation of reactive oxygen species (ROS) such as highly diffusible hydrogen peroxide (H2O2) is a hallmark of oxidative stress during inflammatory lung conditions including pneumonia. However, the impact of exogenous ROS on NET formation and the signaling pathway involved in the process is not completely understood. Here we demonstrate that the ROS‐sensing, non‐selective, calcium‐permeable channel transient receptor potential melastatin 2 (TRPM2) is required for NET formation in response to exogenous H2O2. This TRPM2‐dependent H2O2‐mediated NET formation involved components of autophagy and activation of AMPK and p38 MAPK, but not PI3K and AKT. Primary neutrophils from Trpm2–/– mice fail to activate this pathway with a block in NET release and a concomitant decrease in their antimicrobial capacity. Consequently, Trpm2–/– mice were highly susceptible to pneumonic infection with Klebsiella pneumoniae owing to an impaired NET formation and high bacterial burden despite increased neutrophil infiltration in their lungs. These results identify a key role of TRPM2 in regulating NET formation by exogenous ROS via AMPK/p38 activation and autophagy machinery, as well as a protective antimicrobial role of TRPM2 in pneumonic bacterial infection.—Tripathi, J. K., Sharma, A., Sukumaran, P., Sun, Y., Mishra, B. B., Singh, B. B., Sharma, J. Oxidant sensor cation channel TRPM2 regulates neutrophil extracellular trap formation and protects against pneumoseptic bacterial infection. FASEB J. 32, 6848–6859 (2018). www.fasebj.org