bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of changes in the intensity of polished surface glow of natural stone depending on the intensity of its heating
Authors: Korobiichuk V V , Kryvoruchko A O , Bilobrov D M 
Published: December 2024

Abstract
In this publication, the authors consider the influence of high temperatures on the change in the intensity of the gloss of the front surface of products made of acidic and basic rocks of natural stone. Changes in gloss intensity were analyzed for each of the control points of heating for the front surface of samples of acidic and basic rocks of natural stone. The main factors affecting the change in the intensity of the gloss of the front surface of products made of acidic and basic natural stone are determined. It has been established that when heated (exposure to high temperatures), acidic rocks of natural stone lose their luster less intensively than rocks with a basic composition. The greatest loss of luster upon heating (exposure to high temperatures) is characteristic of labradorite. On the basis of the obtained values, it is possible to predict the change in the intensity of the gloss of the front polished surface of products from basic and acidic natural stone after exposure to high temperatures, as well as to control the quality of the front surface of natural stone products to obtain products with specified parameters for restoration or restoration work.
The influence of the war on the content of some components in the rivers of Ukraine
Authors: Litynska M I , Pelekhata O B 
Published: December 2024

Abstract
As a result of hostilities, the surface waters of Ukraine may be polluted by various components. This can significantly affect the safety of drinking water supply and the ability of water bodies to self-clean. The article is devoted to the calculation of modified water pollution indices and the assessment of water pollution for some Ukrainian rivers that may have been affected by the war. We calculated the modified water pollution index and assessed water pollution for 7 points of 5 Ukrainian rivers (Desna, Dnipro, Styr, Siversky Donets and Oskil) that may have been affected by the war. The modified water pollution index also included nitrates and polyphosphates, unlike the conventional water pollution index. River conditions were assessed from 2018 to 2023 in order to compare water quality in these rivers before and after active hostilities began in February 2022. The most significant effect of military operations on water quality in 2022 was seen at the Siversky Donets and Oskil rivers in the Kharkiv and Donetsk regions, where there were numerous ongoing hostilities. Pollution by biogenic components did not exceed the self-cleaning capacity of these rivers, but bioremediation methods can be used to accelerate the return to normal conditions. Despite being in a conflict zone in the spring of 2022, this had minimal impact on the water quality of the Desna and Dnipro rivers near Kyiv in the summer of 2022. The water quality of the Styr river was improved due to absence of active military actions near the river and significant decrease in tourism and agricultural use of territories.
Assessment of the distribution and population characteristics of Musculium lacustre Müller, 1774 (Mollusca, Bivalvia, Sphaeriidae) in anthropogenically altered aquatic landscapes of the northern right bank of the Dnipro River in Ukraine
Authors: Shevchuk L M , Bylyna L V , Vasilieva L A , Herasymchuk O L , Vakaliuk T A 
Published: December 2024

Abstract
Today, the rapid disappearance of bivalves in European water bodies is a matter of serious concern. The state of study of mollusks of the family Sphaeriidae in Europe, including Ukraine, is considered insufficient, which does not allow to assess their resources, develop measures for protection and restoration of their numbers. The paper assesses the distribution and frequency of occurrence of the small bivalve Musculium lacustre Müller, 1774 (Mollusca, Bivalvia, Sphaeriidae) in the anthropogenically altered aquatic landscapes of the northern right bank of the Dnipro River of Ukraine. This species is considered to be quite eutopian, but in the surveyed region it shows a clear tendency to decrease the frequency of occurrence and population density of individuals. The reason for this situation is the disappearance of typical habitats due to the transformation of rivers into a system of ponds and reservoirs as a result of dam construction and pollution. Out of 232 sites analyzed, M. lacustre was found in the Pripyat sub-basin in only 5 sites, and in the Teteriv basin (Middle Dnipro sub-basin) in only 2 sites (the frequency of occurrence in these sub-basins is 4% each, and the overall figure for the sampling area is 3%. The maximum population density was 21 in the Hnylopyat River, 11 in the Gusak River, and 1-3 ind./m2 in other sampling sites, although according to literature data, in the last century it could reach several thousand per m2 in Ukraine. Found in rivers and streams, it prefers flowing areas.
Regulation of water flow on the foothills on the Ukrainian Carpathians rivers with the use of flooded groynes
Authors: Shynkaruk L A , Volk L R , Dovbenko I E 
Published: December 2024

Abstract
Based on the results of extensive laboratory studies of regulatory and protective structures – groynes, carried out in the hydrotechnical laboratory of National University of Water and Environmental Engineering (NUWEE), recommendations for the design, construction, and operation of structures have been developed. The method of hydraulic calculation of groynes is presented. According to these recommendations, a system of structures with experimental types of groynes on the foothills of the rivers of the Ukrainian Carpathians was designed and built. Surveys were carried out on objects of various types of groynes after the passage of floods on the rivers of the specified region. The causes of deformations of buildings and their destruction have been established. The influence of the existing groynes on the channel process has been studied. Conclusions were drawn on the further use of groynes structures as regulatory and protective structures in the foothills of rivers.
Concession as a tool for improving the budget and tax incentives for investment attractiveness of the tourist complex in Ukraine
Authors: Krushinska A V , Samaricheva T A , Arziantseva D A , Zakharkevych N P , Hrytsenko O M 
Published: December 2024

Abstract
The argumentation of the use of concession of historical and architectural objects in Ukraine as a tool for improving the budget and tax incentives for investment attractiveness of the tourist complex has been carried out. A scientific approach to budgetary and tax incentives for investment attractiveness of tourist facilities has been formed using an optimization model for calculating concession payments, which ensures the flexibility of incentives, takes into account the interests of investors, based on indicators for assessing the effectiveness of an investment project with a simultaneous focus on maximizing budget revenues. It is substantiated that the budgetary and tax incentives for investment attractiveness of tourist complexes through the introduction of public-private partnership in the form of a concession of historical and architectural objects will allow private business to invest in attractive tourist sites and ensure higher profitability of the hotel and restaurant business, since the interests of the investor are taken into account, and will also reduce budget expenditures and increase tax revenues to the budgets from servicing tourist flows.
The impact of low-molecular carboxylic acids on carbon dioxide corrosion of steel in underground gas production equipment in the Dnipro-Donetsk basin fields
Authors: Dmytrenko V I , Zezekalo I G , Peremetchyk A V , Matsui A M 
Published: December 2024

Abstract
The work carried out a set of experimental studies to reduce the activity of corrosion processes in underground well equipment. Corrosion of downhole equipment is a complex heterogeneous process. In the complex of factors influencing both magnitude and intensity of corrosion damage, low molecular weight water-soluble carboxylic acids play a significant role. The features of metal-carbon dioxide corrosion (CO2 corrosion) in production well fluid have been established depending on low molecular weight carboxylic acids. It has been revealed that the influence of carboxylic acids in field wells in the initial period of development is reduced to the destruction of protective carbonate films formed as a result of carbon dioxide corrosion of the metal surface. The highest corrosion rate is at the beginning of droplet water condensation since the main factors influencing the corrosion rate (acid concentration and temperature) are greatest at this point. It has been established that the interaction of organic acids with metal occurs in the Dnipro-Donetsk basin fields at the temperature range of 90-110°C and a depth of 3000-3500 m. The interval of action of organic acids on the metal of tubing pipes depends on the regime constancy and operating time of the well. In constant mode, the effect of organic acids is limited to 100-150 m. The effect of corrosion under the influence of organic acids is especially pronounced in wells where the carbon dioxide content is insignificant. The influence of corrosion products on the process speed has been determined. Iron salts of carboxylic acids, formed as a result of corrosion during condensation of aqueous fluid in a well, are subsequently inhibitors of carbon dioxide corrosion of gas industry equipment.
The current state and development prospects of the graphite industry of Ukraine
Authors: Lozhnikov O V , Pavlychenko A V , Shustov O O , Dereviahina N I 
Published: December 2024

Abstract
The article is devoted to establishing the current state of graphite deposits development in Ukraine and the prospects of supplying industry with this critical raw material. The countries with the largest production of natural graphite and Ukraine’s share of the world market have been determined. Global trends in graphite consumption in industry and its impact on market value have been established. The mining and geological conditions of typical graphite deposits occurrence have been determined, which indicates the expediency of surface mining. The geological characteristics of the largest graphite deposits of Ukraine have been studied, which allows further research to determine directions for increasing the efficiency of beneficiation of graphite raw materials and to increase the greening of production. The main areas of graphite use in high-tech sectors of the economy are given, which confirms the critical role of this raw material in the creation of renewable energy chains. Estimated reserves of graphite have been established in the most significant graphite deposits of Ukraine, which include Zavallivske, Petrivske, Troitske, Mariupolske, Balakhivske and Burtynske. The estimated content of graphite in the ore of the main deposits has been determined, which makes it possible to forecast the country’s resource availability with this critical raw material in the future.
Ecological Risk Assessment of Heavy Metals in the Soil at reclaimed solid waste landfill
Authors: Serafimova Ekaterina 
Published: December 2024

Abstract
Environmental pollution with heavy metals is a problem for most developing or emerging countries. The study investigated the concentrations of arsenic (As), nickel (Ni), chromium (Cr), nickel (Ni), zinc (Zn), cobalt (Co), copper (Cu), and lead (Pb) in 10 samples of reclaimed soil for environmental risk assessment and spatial distribution following The Municipal Solid Waste Depot in Central Northern Bulgaria. According to Bulgarian legislation, the measured average concentrations of arsenic are below the maximum permissible concentration. Using the obtained heavy metal data, pollution indices such as geoaccumulation index (Igeo), pollution factor (CF), ecological risk factor (Er), pollution degree (Cdeg), and pollution index (PLI) were calculated and the results obtained categorized the site as uncontaminated. This suggests that the studied area is well-reclaimed and does not pose an ecological risk. The findings presented in this work support data on heavy metal contamination and risk assessment in Bulgaria. This research may have benefits for trace heavy metal pollution prevention and future monitoring studies of the region.
Effect of HIV and Malaria in Pregnancy on Pertussis-specific Antibodies and Transplacental Antibody Transfer: A Secondary Analysis of a Prospective Cohort Study in Mozambican Pregnant Women and Their Infants
Authors: Bardají Azucena , Dobaño Carlota , Alonso Selena , Vala Anifa , Pantoja P. Efrain , Vidal Marta , Maculuve Sonia , Nhacolo Arsenio , Rupérez María , Morató Alba , Quintó Llorenç , Sevene Esperança , Macete Eusebio , Mayor Alfredo , Menéndez Clara , Moncunill Gemma , González Raquel 
Published: December 2024

Background:
Infection during pregnancy may affect maternal and infant immunity against childhood diseases. We aimed to evaluate the effects of maternal HIV and malaria on maternal and infant pertussis immunity and placental antibody transfer.

Methods:
A prospective study was conducted in mother-infant pairs in Mozambique. Peripheral and cord blood samples were collected for pertussis-specific immune assays. Maternal HIV serostatus and Plasmodium falciparum infection were assessed. The placental transfer was assessed using cord-to-mother ratios of IgG against pertussis toxin (PT), pertactin (PRN) and fimbriae 2/3 (FIM).

Results:
A total of 270 mother-infant pairs were included: 99 mothers with HIV and 40 mothers with malaria. Pregnant women with HIV showed a reduction in placental transfer [PT: 12.7%, 95% confidence interval (CI): 2.6–21.7, P = 0.015; PRN: 14.6%, 95% CI: 6.3–22.1, P = 0.001; and FIM: 7.5%, 95% CI: −6.6 to 19.7, P = 0.282] compared with women without HIV. A trend toward reduction in IgG transfer was observed among women with malaria (PT: 9.5%, 95% CI: −4.2 to 21.4, P = 0.165; PRN: 5.0%, 95% CI: −7.0 to 15.7, P = 0.394; and FIM: 15.9%, 95% CI: −0.9 to 30.0, P = 0.062) compared with those without. Maternal HIV infection (odds ratio: 4.43, 95% CI: 2.14–9.1; P < 0.001) and high viral load (odds ratio: 4.37, 95% CI: 1.4–12.2; P = 0.033) were associated with impaired placental transfer.

Conclusions:
Maternal HIV infection is associated with lower mother-to-infant transfer of pertussis antibodies. While efforts continue in the health care of pregnant women with HIV, interventions such as maternal immunization can be a valuable strategy to prevent pertussis in infants.
Optimizing the Evaluation of Letters of Recommendation for Obstetrics and Gynecology Residency Applicants
Authors: Morales-Ramirez Pedro , Gutierrez Erick , DeMier Brettany , White-Hammonds Preslie , Mishek Henry , Dasgupta Arhita 
Published: December 2024

OBJECTIVE:
To develop and validate a ChatGPT-powered tool for assessing letters of recommendation in obstetrics and gynecology residency applicants.

METHODS:
This study was conducted in two phases at a university-based obstetrics and gynecology residency program. During the development phase, 30 letters of recommendation were used to create ChatGPT prompts based on a validated scoring rubric that assesses phrases, letter features, and applicant abilities. ChatGPT scores were compared with benchmarks using intraclass correlation coefficients (ICCs) to evaluate reliability, and ChatGPT processing times were measured against those of human reviewers. In the second phase (validation), 88 letters of recommendation from residents with and without academic deficiencies (n=30) were analyzed using the ChatGPT prompts developed and refined in the first stage. Residents with academic deficiencies (case group) were matched 1:1 with a control group of residents without noted deficiencies by medical school, gender, and training period. The letter of recommendation scores between the case and control groups then were compared using the Mann-Whitney U test.

RESULTS:
ChatGPT significantly reduced review time per letter, from 7.2 minutes to 45 seconds. Inter-rater reliability between ChatGPT and benchmark scores indicated strong agreement for specific phrases (ICC 0.82, 95% CI, 0.75–0.88), moderate agreement for letter features (ICC 0.75, 95% CI, 0.68–0.82), and fair agreement for applicant abilities (ICC 0.70, 95% CI, 0.62–0.78). After refinement, ICCs improved to 0.92 (95% CI, 0.88–0.95), 0.81 (95% CI, 0.74–0.87), and 0.77 (95% CI, 0.69–0.84). Mean letter of recommendation scores for specific phrases differed significantly between residents with deficiencies (4.49; 95% CI, 3.29–5.68) and residents in the control group (6.00; 95% CI, 5.00–7.00) (P=.017). Subgroup analysis showed an even greater gap between residents with multiple deficiencies (2.15; 95% CI, 1.42–3.38) and residents in the control group (6.00; 95% CI, 5.00–7.00; P<.001), suggesting a higher predictive value for at-risk residents.

CONCLUSION:
ChatGPT can enhance the residency selection process by providing reliable letter of recommendation analysis significantly faster than human reviewers and effectively identifying key phrases in letters of recommendation that could predict future academic deficiencies.
Enhanced methods for genetic assays inDrosophilacells
Authors: Wang Y , Lee JY , Housden AE , Hottinger E , Housden BE 
Published: December 2024

ABSTRACTGenetic assays are an invaluable tool for both fundamental biological research and translational applications. Variable Dose Analysis (VDA) is an RNAi-based method for cell-based genetic assays that offers several advantages over approaches such as CRISPR and other RNAi-based methods including improved data quality (signal-to-noise ratio) and the ability to study essential genes at sub-lethal knockdown efficiency. Here we report the development of three new variants of the VDA method called high-throughput VDA (htVDA), VDA-plus and pooled-VDA. htVDA requires 10-fold reduced reagent volumes and takes advantage of liquid handling automation to allow higher throughput screens to be performed while maintaining high data quality. VDA-plus is a modified version of VDA that further improves data quality by 4.5-fold compared to standard VDA to allow highly sensitive detection of weak phenotypes. Finally, Pooled VDA allows greatly increased throughput by analysing multiple gene knockdowns in a single population of cells. Together, these new methods enhance the toolbox available for genetic assays, which will prove valuable in both high-and low-throughput applications. In particular, the low noise and ability of VDA to study essential genes at sub-lethal knockdown levels will support identification of novel drug-targets, among which essential genes are often enriched. While these tools have been developed inDrosophilacells, the underlying principles are transferrable to any cell culture system.
High-dimensional spectral flow cytometry of activation and phagocytosis by peripheral human polymorphonuclear leukocytes
Authors: Lamb Evan R. , Glomski Ian J. , Harper Taylor A. , Solga Michael D. , Criss Alison K. 
Published: December 2024

AbstractPolymorphonuclear lymphocytes (PMNs) are terminally differentiated phagocytes with pivotal roles in infection, inflammation, tissue injury, and resolution. PMNs can display a breadth of responses to diverse endogenous and exogenous stimuli, making understanding of these innate immune responders vital yet challenging to achieve. Here, we report a 22-color spectral flow cytometry panel to profile primary human PMNs on population and single cell levels for surface marker expression of activation, degranulation, phagocytosis, migration, chemotaxis, and interaction with fluorescently labeled cargo. We demonstrate the surface protein response of PMNs to phorbol ester stimulation compared to untreated controls in an adherent PMN model with additional analysis of intra- and inter-subject variability. PMNs challenged with the Gram-negative bacterial pathogenNeisseria gonorrhoeaerevealed infectious dose-dependent changes in surface marker expression in bulk, population-level analysis. Imaging flow cytometry complemented spectral cytometry, demonstrating that fluorescence signal from labeled bacteria corresponded with bacterial burden on a per-cell basis. Spectral flow cytometry subsequently identified surface markers which varied with direct PMN-bacterium association as well as those which varied in the presence of bacteria but without phagocytosis. This spectral panel protocol highlights best practices for efficient customization and is compatible with downstream approaches such as spectral cell sorting and single-cell RNA-sequencing for applicability to diverse research questions in the field of PMN biology.Summary SentenceHere we report a 22-color spectral flow cytometry panel to profile primary human PMNs for markers of activation, degranulation, phagocytosis, migration, and chemotaxis using phorbol ester stimulation and bacterial challenge as proofs-of-concept.
Ex-vivo expansion of patient-derived PBMCs preferentially results in effector memory T-cell proliferation with restored autologous efficiency in HBV-HCC
Authors: Kah Janine , Staffeldt Lisa , Mattert Gregor , Volz Tassilo , Voß Maximilian , Schulze Kornelius , Heumann Asmus , Dandri Maura , Lüth Stefan 
Published: December 2024

AbstractBackgroundT-cell-based therapies achieved milestones in targeting solid tumors, such as hepatocellular carcinoma, by leveraging the cytotoxic potential of effector memory T-cells. However, a key challenge lies in the ex-vivo expansion of functional memory T-cells while simultaneously preventing over-differentiation into senescent TEMRA cells.MethodsPBMC derived from 10 HCC patients and two healthy donors were used for expansion in small bioreactors equipped with a permeable membrane for 2 weeks. During expansion, surface marker composition, cytokine and chemokine production were observed. Enriched T-cell subsets from three patients with chronic HBV infection were analysed in detail. We extracted the enriched complement of one chronically infected patient to elucidate the therapeutic potential.ResultsWe successfully expanded the effector T-cell subsets for all investigated samples and consistently enriched the cell amount over time. Subsequently, we showed that the expanded patient-derived T-cells showed functionality against autologous liver cancer-derived cells by inducing receptor and protein-mediated cell death.ConclusionWe showed the consistent ex-vivo expansion of T-cell subsets of initial patient-derived PBMCs. The enriched subsets exhibit cytotoxic functionality and shift to cytolytic TEMRA cells in an immune evasion setting in the context of chronic HBV-infected patient-derived liver cancer cells.
abCAN: a Practical and Novel Attention Network for Predicting Mutant Antibody Affinity
Authors: Gong Chen , Shen Yunyao , Liu Hongde , Ming Wenlong 
Published: December 2024

AbstractAccurate prediction of mutation effects on antibody-antigen interactions is critical for antibody engineering and drug design. In this study, we present abCAN, a practical and novel attention network designed to predict changes in binding affinity caused by mutations. abCAN requires only the pre-mutant antibody-antigen complex structure and mutation information to perform its predictions. abCAN introduces an innovative approach, Progressive Encoding, which progressively integrates structural, residue-level, and sequential information to construct the complex representation in a systematic manner, effectively capturing both the topological features of the structure and contextual features of the sequence. During which, extra weight to interface residues would also be applied through attention mechanisms. These learned representations are then transferred to a predictor that estimates changes in antibody-antigen binding affinity induced by mutations. On the benchmark dataset, abCAN achieved a root-mean-square error (RMSE) of 1.195 (kcal/mol-1) and a Pearson correlation coefficient (PCC) of 0.841, setting a new state-of-the-art (SOTA) benchmark for prediction accuracy in the field of antibody affinity prediction.