bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resilient urbanization for water limited environments
Authors: Jenerette G. Darrel 
Published: December 2024

Abstract
Dryland cities are important locations for human–environmental interactions and differ in many key characteristics from cities in wetter environments. Defined by chronic water deficit, these cities face challenges that include securing essential resources, reducing vulnerability to hazards and conserving threatened species. The resilience of dryland cities depends on interactions across the entire urban continuum, from urban cores and suburban areas to teleconnected zones and wildland–urban interfaces. Resilience solutions must enhance the well-being of residents and institutions while fostering adaptive capacity throughout the urban continuum. Key axes of solutions include hydrologic integration, including stormwater capture and reuse, nature-based solutions, including expanding urban tree cover for cooling and health benefits, and landscape sustainability, including the incorporation of spatial heterogeneity into planning and development. Addressing the large uncertainties in ensuring more resilient cities requires convergence research, the integration of theoretically driven science that brings researchers and stakeholders together to identify problems, solutions and opportunities for action. While convergence approaches look to address pressing scientific uncertainties, they also are inherently place-based and address compelling case studies to understand system dynamics and improve decision-making and land management. New research is needed to address the trade-offs resulting from decision-making and urban management activities, to meet the needs of diverse stakeholders and to ensure that policies do not marginalize underserved communities. By leveraging innovative technologies, sustainable practices and community involvement, dryland cities can overcome the challenges posed by chronic water limitations and thrive in their diverse environments.
Genotyping of benzimidazole resistance using β-tubulin isotype 1 marker in Haemonchus contortus of sheep and goats in Paraná, Southern Brazil
Authors: do Prado C. Melchior , Rodrigues J. Ferreira Vasconcelos , Frota G.A. , Vieira D.L. , Monteiro J.P. , Molento M. Beltrão 
Published: December 2024

Abstract
Haemonchus contortus is one of the most pathogenic gastrointestinal parasites that infect small ruminants. The indiscriminate use of anthelmintics (i.e., benzimidazole class, BZ) to control infections has led to the reduction of drug efficacy in H. contortus populations worldwide. Resistance to BZ is associated with high frequencies of single nucleotide polymorphisms at F200Y, F167Y, and E198A positions of the β-tubulin isotype 1 gene. This study aimed to determine the frequency of single nucleotide polymorphisms associated with BZ resistance in H. contortus from 18 farms (545 sheep and 124 goats) in Paraná, Southern Brazil. Health management practices were identified as risk factors from individual farms. Genomic DNA was extracted from 20,000 larvae/farm and used in quantitative polymerase chain reaction assays for the three mutations. We ran a correlation analysis between flock health and quantitative polymerase chain reaction data. H. contortus was the most prevalent parasite in 67% (12/18) of the farms. Resistant allele frequencies were detected for F200Y (var. 46.4 to 72.0%) and F167Y (var. 15.7 to 23.8%). Only (100.0%) susceptible alleles were detected for the E198A. High treatment frequency (15/18), visual weight estimations for anthelmintic dose (15/18), no integration with other farm practices (14/18), treatment of all animals (14/18), and no quarantine period for newly acquired animals (10/18) were considered the most critical risk factors associated with BZ resistance. This is the first systematic prevalence study linking management practices on smallholder farms and the molecular data of BZ resistance of H. contortus in Southern Brazil.
Behavioural alterations in surface and cave populations of isopod crustacean Asellus aquaticus by Acanthocephalus anguillae
Authors: Benko G. , Fišer Ž. , Kostanjšek R. 
Published: December 2024

Abstract
Acanthocephalans are obligatory endoparasites that often alter the phenotype of their invertebrate intermediate host to facilitate trophic transmission to their final vertebrate host. Acanthocephalus anguillae, a widespread parasite of European freshwater fishes and isopod Asellus aquaticus, was recently discovered also in Postojna-Planina Cave System (Slovenia) parasitising olms (Proteus anguinus) and cave populations of A. aquaticus. This setting offers a unique opportunity to investigate potential fine-tuning of parasitic manipulations to the specifics of the highly divergent subterranean environment where some common phenotypic alterations lose functionality, but others might gain it. We measured three behavioural traits: movement activity, shelter-seeking, and response to light of infested and uninfested isopods from surface and cave populations. All behaviours were quantified from 1-h video-recordings via video-tracking isopod’s movement in empty or custom modified (half-sheltered/half-illuminated) Petri dishes. Infested isopods of both populations spent significantly less time sheltering and were significantly less photophobic than uninfested ones, whereas the activity of isopods was not altered. However, we observed almost no cave-specific responses upon infestation in the two altered behaviours. It seems phenotypic alterations are not particularly fine-tuned to the subterranean environment and its hosts, and likely still reflect the parasite’s surface origin.
Characterisation and serodiagnostic evaluation of a recombinant 22.6-kDa tegument protein of Schistosoma spindale
Authors: Priya M.N. , Bindu L. , Pradeep M.A. , Nisha E.A. , Amrutha A. , Nikitha S. , Asha R. , Shynu M. , Devada K. 
Published: December 2024

Abstract
Schistosomosis in animals due to Schistosoma spindale significantly burdens India’s livestock economy because of high prevalence and morbidity and is mostly underdiagnosed from the lack of sensitive tools for field-level detection. This study aimed to clone, express the 22.6-kDa tegument protein of S. spindale (rSs22.6kDa) and to utilise it in a dot enzyme-linked immunosorbent assay for serodiagnosis. RNA was extracted from adult worms recovered from the mesenteries of slaughtered cattle to amplify the gene encoding the 22.6-kDa protein. In silico analysis revealed the protein’s secondary structure, consisting of 190 amino acids forming alpha helices (47.89%), extended strands (17.37%), beta turns (8.95%), and random coils (25.79%), with α helices and β sheets in the tertiary structure. Two conserved domains were noted: an EF-hand domain at the N-terminus and a dynein light-chain domain at the C-terminus. Phylogenetic studies positioned the S. spindale sequence as a sister clade to Schistosoma haematobium and Schistosoma bovis. The gene was cloned into a pJET vector and transformed into Escherichia coli Top 10 cells, with expression achieved using a pET28b vector, BL21 E. coli cells, and induction with 0.6 mM isopropyl-β-d-thiogalactopyranoside. The protein’s soluble fraction was purified using nickel-chelating affinity chromatography, confirmed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and immunoblotting, identifying a distinct immunodominant 22.6-kDa protein. The diagnostic utility was validated using a dot enzyme-linked immunosorbent assay which demonstrated a of sensitivity of 89.47% and specificity of 100%. The study records for the first time the prokaryotic expression and evaluation of the 22.6-kDa tegumental protein of S. spindale, highlighting its potential as a diagnostic antigen for seroprevalence studies in bovine intestinal schistosomosis.
The revival of psilocybin between scientific excitement, evidence of efficacy, and real-world challenges
Authors: Scala Mauro , Fabbri Chiara , Fusar-Poli Paolo , Di Lorenzo Giorgio , Ferrara Maria , Amerio Andrea , Fusar-Poli Laura , Pichiecchio Anna , Asteggiano Carlo , Menchetti Marco , De Ronchi Diana ,   MNESYS - Mood and Psychosis Sub-Project (Spoke 5), Fanelli Giuseppe , Serretti Alessandro 
Published: December 2024

AbstractThe revival of psilocybin in psychopharmacological research heralds a potential paradigm shift for treating mood and anxiety disorders, and other psychiatric conditions beyond the psychotic spectrum. This critical review evaluates current evidence on psilocybin’s efficacy, juxtaposing potential benefits with the practical aspects of psychedelic-assisted psychotherapy (PAP) and the methodological constraints of existing research.An electronic literature search was conducted using PubMed/MEDLINE, selecting studies published up to December 2023 that explored the clinical use of psilocybin in mood and anxiety disorders, obsessive-compulsive disorder, post-traumatic stress disorder, and substance use disorder. Despite promising preliminary results suggesting psilocybin’s efficacy in alleviating depression and anxiety, as well as obsessions, compulsions, and addictive behaviors, significant evidence gaps persist. These include evaluating the efficacy of psilocybin compared to standard antidepressants or anxiolytic molecules and identifying patient subpopulations that might benefit most from PAP. Concerns about psilocybin’s safety, long-term efficacy, and optimal dosage remain unclear due to previous trials’ limitations. Real-world implementation faces challenges, including infrastructural requirements, personnel training, and unresolved legal and ethical issues. This paper argues for further research to substantiate the evidence base, emphasizing the need for larger studies that overcome current methodological limitations and explore psilocybin’s full therapeutic potential. While psilocybin holds promise for psychiatry, its successful translation from research to clinical practice demands more robust evidence on efficacy, safety, and methodological rigor. In addition, other factors, such as cultural stigma and legal/ethical issues, need to be successfully addressed to facilitate psilocybin’s implementation in healthcare systems.
The Evolution of Parallel Electron Temperature in Magnetospheric Reconnection Inflows
Authors: Gershman Daniel J. , Chen Li‐Jen , Le Ari , Shuster Jason , Dorelli John C. , Ng Jonathan , Giles Barbara , Viñas Adolfo F. , Torbert Roy , Burch James L. 
Published: December 2024

AbstractUsing data from NASA's Magnetospheric Multiscale mission captured in a reconnection inflow on the magnetospheric side of Earth's dayside magnetopause, we find a region where the heat flux density gradient term balances the parallel compression term in the electron parallel temperature equation. Combining these observations with analysis of the generalized fluid equations indicates that such a behavior represents a quasi‐isothermal region, where cold magnetosheath beams that have transported across the magnetopause introduce non‐zero gradients in parallel heat flux density. This region should prevail near dayside reconnection X‐lines in inflows on the magnetospheric side due to the formation of mixed electron distributions and increased parallel temperatures that arise from three‐dimensional boundary dynamics.
Increased Predictability of Extreme El Niño From Decadal Interbasin Interaction
Authors: Ma Xuan , Liang Rizhou , Chen Xiaosong , Xie Fei , Zuo Jinqing , Sun Cheng , Ding Ruiqiang 
Published: December 2024

AbstractPredicting extreme El Niño–Southern Oscillation (ENSO) events remains a formidable task. Utilizing eigen microstates (EMs) of complex systems, we elucidate the interplay of two key sea surface temperature (SST) anomaly modes, the newly identified Atlantic‐Western Pacific Multidecadal mode (AM‐WPM) and discovered Pacific Meridional Mode (PMM). Our findings demonstrate that a cold AM‐WPM phase coupled with a positive PMM phase markedly elevates the probability of extreme El Niño events; AM‐WPM's decadal variability serves as a key modulator of extreme El Niño events' frequency. Our empirical model, capitalizing on these modes, achieves robust forecasts with a 6–8 months lead time and boasts a 0.73 correlation with the observed ENSO index in hindcasts. Notably, the model precisely forecasts the intensity of four extreme El Niño episodes: 1982/1983, 1987/1988, 1997/1998, and 2015/2016. Our findings offer promising avenues for refining ENSO predictive frameworks and deepen our understanding of the key climatic drivers.
New Zealand Southern Alps Blanketed by Red Australian Dust During 2019/2020 Severe Bushfire and Dust Event
Authors: Winton V. Holly L. , Charlier Bruce L. A. , Jolly Ben H. , Purdie Heather , Anderson Brian , Hunt John E. , Dadic Ruzica , Taylor Sally , Petherick Lynda , Novis Phil M. 
Published: December 2024

AbstractEpisodic deposition of light absorbing impurities on glaciers reduces albedo and exacerbates snow melt. In 2019/2020 a devastating Australian bushfire and desert dust event combined with favorable meteorological conditions transported an unprecedented mass of impurities across the Tasman Sea turning the Southern Alps of Aotearoa New Zealand red. Here we use time lapse cameras, airmass back trajectories, snow impurity geochemistry, and remote sensing to quantify the timing, provenance, and mass deposition of the event. Deposited in late November 2019, the impurities were dominated by mineral dust with a distinct southeastern Australian geochemical fingerprint. The event deposited ∼4,500 ± 500 tons of red dust to Southern Alps permanent snow and ice with a mean dust mass concentration of 6.5 ± 0.7 g m−2. A southeast Australian desert dust storm generated by the same type of meteorological conditions as the 2020 New Year bushfires was the main driver of the glacier discoloration.
Using Digital Camera and Eddy Covariance Data to Track Vegetation Phenology and Carbon Dioxide Fluxes in the Badain Jaran Desert
Authors: Meng Nan , Wang Nai’ang , Zhao Liqiang , Lv Haoyun , Chen Xiaowen , Yang Ping , Lee Sung‐Ching 
Published: December 2024

AbstractUnderstanding on relationships between seasonality of vegetation phenology and photosynthesis is lacking for desert ecosystems. We used digital camera (i.e., PhenoCam) to monitor the phenology of forest (i.e., 2 sites with one being closer to a lake) and grassland (i.e., 1 site) ecosystems in the Badain Jaran Desert, China. The vegetation phenology was quantified using vegetation indices calculated from the red, green, and blue digital numbers in images obtained by the PhenoCams. Additionally, various meteorological variables were continuously measured, and gross primary production (GPP) was obtained using the eddy covariance technique at the grassland site. The difference between the phenological periods extracted from the PhenoCam images and the artificial visual method was small (≤6 days), indicating that the digital camera can effectively obtain desert vegetation phenology. The key meteorological factors affecting changes in the vegetation indices were identified, with temperature being the most important factor (i.e., correlation coefficients = 0.4–0.8 and p‐value < 0.001 for all three study sites). Although precipitation showed weak correlation with the vegetation index (correlation coefficient = 0.18–0.14, p‐value < 0.01), rapid increases in the vegetation index were observed in response to precipitation events. Vegetation indices were strongly correlated with GPP variations at the grassland, and the strongest correlation was observed in the green‐up stage (correlation coefficient = 0.67 to 0.85, p‐value < 0.001). The highest GPP lagged about 1 month behind the peak in the vegetation indices in summer (June–August). Our results can markedly improve the knowledge of desert ecosystem processes and aid in assessing the influence of future climate changes in drylands.
Neural basis of adolescent THC-induced potentiation of opioid responses later in life
Authors: Hubbard Elizabeth , Derdeyn Pieter , Galinato Vivienne Mae , Wu Andrew , Bartas Katrina , Mahler Stephen V. , Beier Kevin T. 
Published: December 2024

AbstractUse of one addictive drug typically influences the behavioral response to other drugs, either administered at the same time or a subsequent time point. The nature of the drugs being used, as well as the timing and dosing, also influence how these drugs interact. Here, we tested the effects of adolescent THC exposure on the development of morphine-induced behavioral adaptations following repeated morphine exposure during adulthood. We found that adolescent THC administration paradoxically prevented the development of anxiety-related behaviors that emerge during a forced abstinence period following morphine administration but facilitated reinstatement of morphine CPP. Following forced abstinence, we then mapped the whole-brain response to a moderate dose of morphine and found that adolescent THC administration led to an overall increase in brain-wide neuronal activity and increased the functional connectivity between frontal cortical regions and the ventral tegmental area. Last, we show using rabies virus-based circuit mapping that adolescent THC exposure triggers a long-lasting elevation in connectivity from the frontal cortex regions onto ventral tegmental dopamine cells. Our study adds to the rich literature on the interaction between drugs, including THC and opioids, and provides potential neural substates by which adolescent THC exposure influences responses to morphine later in life.
An efficient method of moments for analysis of electromagnetic scattering from a multilayered arbitrary‐shape anisotropic dielectric object
Authors: Kalantari Mona , Sadeghi Seyed Hossein Hesamedin 
Published: December 2024

AbstractThis paper introduces an efficient method of moments (MoM) designed to explore electromagnetic scattering in multilayered anisotropic structures. Each layer is made up of a dielectric anisotropic material characterised by a generalised tensor for permittivity, which is unrestricted in its geometrical configuration. The authors’ approach analyses each layer independently, employing the surface equivalence theorem to substitute the interfaces between layers with suitable equivalent electric and magnetic surface current densities. The authors derive the necessary surface integral equations (SIEs) for each interface by implementing the proper boundary conditions. The analysis utilises rotated dyadic Green's functions that populate the infinite space with the material properties specific to each anisotropic layer. The rotation angle corresponds to the deviation between the local principal coordinate system of the material and the global coordinate system, which is determined by diagonalising the full dielectric tensor of the respective anisotropic material given in the global coordinate system. To address the SIEs for determining the unknown equivalent electric and magnetic surface current densities, Galerkin's MoM is applied. This involves expanding the unknown surface currents using suitable basis functions, simplifying the issue to a matrix equation solved through the inversion of a block‐tridiagonal impedance matrix. The diagonal nature and sparse structure of the impedance matrix, along with an effective block‐inversion method, significantly boost computational efficiency and reduce memory demands. To demonstrate the feasibility of the proposed method, the authors present a detailed derivation of the impedance matrix for the case of non‐magnetic uniaxial anisotropic media for which the Green's functions are available in closed form. The validity and efficiency of the proposed SIE‐MoM scheme are demonstrated by comparing the results of several case studies against those found in literature and results obtained via commercial numerical codes.
The effect of dynamical states on galaxy cluster populations
Authors: Véliz Astudillo S. , Carrasco E. R. , Nilo Castellón J. L. , Zenteno A. , Cuevas H. 
Published: December 2024

Context. Although the influence of galaxy clusters on galaxy evolution is relatively well understood, the impact of the dynamical states of these clusters is less clear. This series of papers explores how the dynamical state of galaxy clusters affects their galaxy populations’ physical and morphological properties.
Aims. The primary aim of this first paper is to evaluate the dynamical state of 87 massive (M500 ≥ 1.5 × 1014 M⊙) galaxy clusters at low redshifts (0.10 ≤ z ≤ 0.35). This allowed us to obtain a well-characterized sample for analyzing the relevant physical and morphological properties, planned for our next work.
Methods. We employed six dynamical state proxies that utilize optical and X-ray imaging data. We applied a principal component analysis to integrate these proxies effectively, allowing for a robust classification of galaxy clusters into relaxed, intermediate, and disturbed states based on their dynamical characteristics.
Results. The methodology successfully segregates the clusters of galaxies into the three dynamical states. An examination of the projected galaxy distributions in optical wavelengths and gas distributions in X-ray further confirms the consistency of these classifications. The dynamical states of the clusters are statistically distinguishable, providing a clear categorization for further analysis.
Optical assessment of scars after endoscopic mucosal resection of large colorectal polyps in a multicenter, community hospital setting: is routine biopsy still necessary?
Authors: Meulen Lonne W. T. , Bogie Roel M. M. , Siersema Peter D. , Winkens Bjorn , Vlug Marije S. , Wolfhagen Frank H. J. , Baven-Pronk Martine A. M. C. , van der Voorn Michael P. J. A. , Schwartz Matthijs P. , Vogelaar Lauran , Seerden Tom C. J. , Hazen Wouter L. , Schrauwen Ruud W. M. , Herrero Lorenza Alvarez , Schreuder Ramon-Michel , van Nunen Annick B. , de Bruin Gijs J. , Marsman Willem A. , de Bièvre Marc , Roomer Robert , de Ridder Rogier J.J. , Pellisé Maria , Bourke Michael J. , Masclee Ad A. M. , Moons Leon M. G. ,   on behalf of the OPTICAL-STAR Working Group
Published: December 2024

Background Piecemeal endoscopic mucosal resection (EMR) of large (≥ 20 mm) nonpedunculated colorectal polyps (LNPCPs) is succeeded by a 6-month surveillance endoscopy to evaluate the post-EMR scar for recurrence. Data from expert centers suggest that routine tattoo placement and scar biopsies can be omitted, but data from community hospitals are lacking.
Methods The agreement between optical assessment and histological confirmation by routine biopsies was evaluated in a post-hoc analysis of the STAR-LNPCP study (NTR7477), containing prospective data on 6-month post-EMR scar assessments in 30 Dutch community hospitals (October 2019 to May 2022). A standardized protocol was followed for documentation of optical characteristics, imaging, and biopsy of the post-EMR scar.
Results In 1277 post-EMR scar assessments, identification of the scar was achieved in 1215/1277 (95 %). Tattoo placement did not influence scar identification. Scar biopsy was performed in 1050/1215 cases (86 %). Recurrences were seen in 200/1050 cases (19 %). There was good agreement between optical assessment of recurrence and histological confirmation (Cohen’s kappa 0.78 [95 %CI 0.73–0.83]). The negative and positive predictive values were 98 % (95 %CI 97 %–99 %) and 74 % (95 %CI 68 %–80 %), respectively. A higher false-positive rate was seen after prior use of clips (11 % vs. 5 %; P = 0.02). Dedicated endoscopists identified the scar more often (96 % vs. 88 %; P < 0.001), and showed a lower optical recurrence miss rate (1 % vs. 3 %; P = 0.11) compared with nondedicated endoscopists.
Conclusion Based on this multicenter community hospital study, routine tattoo placement and scar biopsies of the post-EMR scar can be omitted. Assessment of post-EMR scars by dedicated endoscopists is advised.