bluuebunny/crossref_metadata_2025_split huggingface dataset:
A case of malignant rhabdoid tumor mimicking yolk sac tumor
Authors: Kubota Yasuo , Arakawa Yuki , Sekiguchi Masahiro , Watanabe Kentaro , Hiwatari Mitsuteru , Kishimoto Hiroshi , Nakazawa Atsuko , Yoshida Akihiko , Ogawa Seishi , Hanada Ryoji , Oka Akira , Takita Junko , Koh Katsuyoshi 
Published: May 2019

AbstractMalignant rhabdoid tumors (MRTs) are rare, highly aggressive embryonal neoplasms caused by biallelic alterations of the SMARCB1 gene. MRTs may occur in any soft tissue, but extracranial extrarenal MRTs are extremely rare. Diagnosis of MRTs in unusual locations and with an uncharacteristic cytomorphology that mimics other tumors is difficult. This was an atypical case of MRT in a 15‐year‐old female with tumors that closely resembled yolk sac tumors. It was extremely challenging to diagnose the tumors without confirming the SMARCB1 status.
Novel heterozygous mutation in the PTEN gene associated with ovarian germ cell tumor complicated by growing teratoma syndrome and overgrowth in a two‐year‐old female
Authors: Tullius Brian P. , Shankar Suma P. , Cole Stacey , Triano Vivien , Aradhya Swaroop , Huang Eric C. , Sanchez Thomas , Pawar Anjali 
Published: May 2019

AbstractGrowing teratoma syndrome (GTS) is a condition in which mature teratoma with negative tumor markers arises at the site of a treated malignant germ cell tumor. Pathogenic variants in PTEN have been reported to cause autosomal dominant cancer predisposition syndromes and are associated with germ cell tumors. We report the association of a novel heterozygous pathogenic variant in PTEN and very early onset ovarian germ cell tumor complicated by GTS as well as overgrowth syndrome. This marks the youngest reported patient to have developed GTS following treatment of her primary malignant ovarian germ cell tumor.
Effects of pharmacist participation in chronic kidney disease (CKD) network and CKD manual distribution on drug‐related kidney injury
Authors: Yamamoto Tatsuo , Nakayama Isao , Kawakatsu Yutaro , Yamamoto Takumi , Ishigami Naoyuki , Watanuki Kei , Okabe Takayoshi , Yokoyama Hidetaro 
Published: May 2019

AbstractPurposeRenin‐angiotensin system (RAS) inhibitors carry a risk of normotensive ischemic acute kidney injury in dehydration and concurrent nonsteroidal anti‐inflammatory drug (NSAID) use. Although the estimated number of patients with chronic kidney disease (CKD) is 20 000, Fujieda, Japan, has only three nephrologists. On 25 March 2016, we reorganized the CKD network to include pharmacists and distributed a CKD manual. We assessed effects of pharmacist participation in the CKD network and CKD manual distribution on patient hospitalizations because of drug‐related kidney injury.MethodsChanges in the prevalence of RAS inhibitor–related estimated glomerular filtration rate (eGFR) declines of greater than or equal to 30% and hyperkalemia of greater than or equal to 6.0 mEq/L in 129 hospitalized CKD patients, drug prescriptions of 14 150 hospitalized patients, and annual medical checkup data in 36 042 citizens were investigated before and after pharmacist participation.ResultsAfter pharmacist participation, patient hospitalizations due to RAS inhibitor–related eGFR declines decreased (71.4% to 38.1%, P = .03) and hyperkalemia declined (38.1% to 9.5%, P = .03). Pharmacist participation influenced the decrease in RAS inhibitor–related eGFR declines (P = .03). NSAID prescriptions decreased (13.4% to 11.8%, P = .003) and acetaminophen prescriptions increased (6.6% to 8.0%, P = .002) among 14 150 hospitalized patients, whereas RAS inhibitor prescriptions decreased (43.2% to 39.4%, P = .002) among 6930 hospitalized patients with eGFR less than 60 mL/min/1.73 m2. A significant number of citizens shifted from CKD stage G3a‐3b to G1‐2.ConclusionsPharmacist participation in the CKD network and CKD manual distribution decreased both hospitalizations due to RAS inhibitor–related kidney injury and citizens with CKD stage G3a‐3b.
Exploratory analysis of treatment response trajectories in the PRISM trial: Models of psychosocial care
Authors: Lau Nancy , Bradford Miranda C. , Steineck Angela , Junkins Courtney C. , Yi‐Frazier Joyce P. , McCauley Elizabeth , Rosenberg Abby R. 
Published: May 2019

AbstractObjectiveAdolescents and young adults (AYAs) with cancer are at high risk of negative psychosocial outcomes. Promoting Resilience in Stress Management (PRISM), a novel, brief, skill‐based intervention, has demonstrated efficacy in improving psychosocial well‐being for AYAs. We utilized data from a recent randomized trial of PRISM versus usual care (UC) to categorize and explore group differences in change trajectories of patient reported outcomes (PROs) over time.MethodsOne hundred English‐speaking AYAs (aged 12‐25 years old) with cancer were randomized to PRISM versus UC. At enrollment and 6 months later, AYAs completed validated PROs measuring resilience (Connor‐Davidson Resilience Scale [CDRISC‐10]), hope (Hope Scale), benefit finding (Benefit and Burden Scale for Children), cancer‐specific quality of life (Pediatric Quality of Life [PedsQL] Cancer Module), and distress (Kessler‐6). Patient response trajectories were categorized as “improved,” “consistently well,” “consistently at risk,” or “deteriorated” using minimal clinically important differences (MCIDs) or established measure cutoffs for all PROs. Positive response trajectories consisted of the first two categories (“improved” and “consistently well”), and negative response trajectories consisted of the latter two categories (“consistently at risk” and “deteriorated”).ResultsAcross all PROs, more patients in the PRISM arm “improved” in psychosocial well‐being over time, and fewer PRISM recipients “deteriorated” over time. Across all PROs, a greater proportion of PRISM participants (vs UC) experienced positive response trajectories. Across all PROs, a greater proportion of UC participants experienced negative response trajectories.ConclusionsPRISM shows evidence of both a prevention effect and an intervention effect. Thus, PRISM may serve as a viable prevention and early intervention model for psychosocial care.
Topical dsRNA delivery induces gene silencing and mortality in the pea aphid
Authors: Niu Jinzhi , Yang Wan‐Jun , Tian Yuan , Fan Jia‐Yao , Ye Chao , Shang Feng , Ding Bi‐Yue , Zhang Jun , An Xin , Yang Li , Chang Teng‐Yu , Christiaens Olivier , Smagghe Guy , Wang Jin‐Jun 
Published: May 2019

AbstractBACKGROUNDWith the growing number of available aphid genomes and transcriptomes, an efficient and easy‐to‐adapt tool for gene function study is urgently required. RNA interference (RNAi), as a post‐transcriptional gene silencing mechanism, is important as a research tool for determining gene functions and has potential as a novel insect control strategy. However, these applications have been hampered by the lack of effective dsRNA delivery approaches in aphids.RESULTSHere, we developed a convenient and efficient dsRNA delivery method, topical RNAi, in aphids. An investigation of its dose and time‐dependent RNAi efficiencies revealed that with as little as 60 ng dsRNA per adult pea aphid (Acyrthosiphon pisum), the indicator gene, Aphunchback, could be significantly silenced within 2 h of exposure. The method was further validated by successfully silencing other different genes, and it was also efficient toward two other aphid species, Aphis citricidus and Myzus persicae. Furthermore, a noticeable mortality was also observed in pea aphids using topical RNAi‐mediated gene silencing, within 4 days post‐dsRNA application for four out of seven tested genes.CONCLUSIONCompared with the currently used dsRNA delivery methods in aphids, microinjection and ingestion, topical RNAi is time‐ and cost‐effective, which could greatly influence RNAi‐based gene functional studies and potential candidate gene selection for developing RNAi‐based aphid control strategies in the future. © 2019 Society of Chemical Industry
Novel combination of a biosurfactant with entomopathogenic fungi enhances efficacy against Bemisia whitefly
Authors: do Nascimento Silva Jaqueline , Mascarin Gabriel M , de Paula Vieira de Castro Rui , Castilho Leda R , Freire Denise MG 
Published: May 2019

AbstractBACKGROUNDMicrobial surfactants are multifunctional surface‐active molecules that have been overlooked in formulating microbial biopesticides. We report a novel approach using the biosurfactant rhamnolipid (RML) against the destructive cosmopolitan insect pest Bemisia tabaci, as well as the combined action of RML with aerial conidia of two entomopathogenic fungi, Cordyceps javanica and Beauveria bassiana. RML was also tested as a suspension agent to improve the recovery rate of conidia from solid substrate for fungal preparations.RESULTSThe recovery rate of conidia increased dramatically (two to five times) with RML compared with a standard surfactant (Tween 80). Spraying solutions of 0.075% and 0.1% (w/v) RML on B. tabaci third instar nymphs induced 100% mortality within 4 days. Conidial suspensions at 5 × 106 conidia/mL amended with RML at 0.01% or 0.05% markedly increased nymphal mortalities and considerably reduced LC50. Conidial suspensions of B. bassiana with 0.05% RML added were more effective against whitefly nymphs (87.3% mortality) than C. javanica + RML (51.4% mortality).CONCLUSIONOur results show that this bacterium‐based RML improved the recovery rate of hydrophobic conidia, and that mixtures of RML with fungal spore suspensions increased their insecticidal activity. © 2019 Society of Chemical Industry
Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post‐translational modification analysis
Authors: Chen Rui , Stupak Jacek , Williamson Sam , Twine Susan M. , Li Jianjun 
Published: May 2019

RationalePorous graphic carbon chromatography (PGC) has a different mechanism in the retention of tryptic peptides compared with reversed‐phase chromatography and in this study we show that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site‐specific glycosylation.MethodsDigests of protein standards (horse myoglobin, bovine fetuin and β‐casein) were analyzed with a capillary liquid chromatography/tandem mass spectrometry (LC/MS/MS) system by coupling an Agilent 1100 HPLC system to a Synapt G2‐Si HDMS (Waters). Peptides were separated using a HyperCarb PGC column (300 μm i.d. × 100 mm) packed with 3 μm particles. MS/MS data were collected in data‐dependent mode and three MS/MS scans were acquired after the full MS scan. RAW data were transformed to .mgf by PLGS (Waters) and searched against the Swissprot database by Mascot. Chromatograms and MS/MS spectra of identified compounds were extracted with Masslynx (Waters) and imported to Origin for analysis. Glycan composition and peptide sequence were manually annotated.ResultsPGC/MS/MS enabled accurate quantitation of the stoichiometry of specific phosphorylation sites from β‐casein by efficient separation of the phosphopeptide and its non‐phosphorylated counterpart, which cannot be achieved by reversed‐phase chromatography. PGC/MS/MS also enabled comprehensive characterization of protein sialoglycosylation as isomeric glycopeptides with different combinations of α2‐3‐ and α2‐6‐linked sialic acids can be separated and the ratios of each combination were verified by exoglycosidase digestion.ConclusionsPGC has demonstrated superior separation of peptides with phosphorylation and glycosylation and can be used as an alternative in the proteomic characterization of post‐translational modifications (PTMs) by polar groups.
Modular field robot deployment for inspection of dilapidated buildings
Authors: Cordie Troy P. , Bandyopadhyay Tirthankar , Roberts Jonathan , Dunbabin Matthew , Greenop Kelly , Dungavell Ross , Steindl Ryan 
Published: May 2019

AbstractRobotic inspection often relies on building custom platforms for each new deployment; this is a luxury that urban search and rescue (USAR) robots do not have when time is of critical importance. A significant factor for robots deployed in disaster areas is the varying size of voids and access ways in their path. These situations require platforms that can quickly reconfigure on location. With these challenges in mind, we present the NeWheel system: An in‐field reconfigurable robotic platform that allows mobility changes before, and during, deployment. The NeWheel platform also has the advantage of being small enough to be person‐deployable and to travel as checked luggage on a commercial flight. This field report presents the results and learnings from three field trips on Peel Island located off the coast of Brisbane, Australia. These field trips featured the deployment of the NeWheel system in multiple configurations to inspect and map inside historic dilapidated buildings. It demonstrates the potential of the NeWheel in buildings cluttered with debris and with unstable flooring whether they are historically important or in USAR contexts.
Seasonal effects of a hydropeaking dam on a downstream benthic macroinvertebrate community
Authors: Mihalicz Jordan E. , Jardine Timothy D. , Baulch Helen M. , Phillips Iain D. 
Published: May 2019

AbstractAs more hydroelectric dams regulate rivers to meet growing energy demands, there is ongoing concern about downstream effects, including impacts on downstream benthic macroinvertebrate (BMI) communities. Hydropeaking is a common hydroelectric practice where short‐term variation in power production leads to large and often rapid fluctuations in discharge and water level. There are key knowledge gaps on the ecosystem impacts of hydropeaking in large rivers, the seasonality of these impacts, and whether dams can be managed to lessen impacts. We assessed how patterns of hydropeaking affect abundance, taxonomic richness, and relative tolerance of BMIs in the Saskatchewan River (Saskatchewan, Canada). Reaches immediately (<2 km) downstream of the dam generally had high densities of BMIs and comparable taxonomic diversity relative to upstream locations but were characterized by lower ratios of sensitive (e.g., Ephemeroptera, Plecoptera, and Trichoptera) to tolerant (e.g., Chironomidae) taxa. The magnitude of effect varied with seasonal changes in discharge. Understanding the effects of river regulation on BMI biodiversity and river health has implications for mitigating the impacts of hydropeaking dams on downstream ecosystems. Although we demonstrated that a hydropeaking dam may contribute to a significantly different downstream BMI assemblage, we emphasize that seasonality is a key consideration. The greatest differences between upstream and downstream locations occurred in spring, suggesting standard methods of late summer and fall sampling may underestimate ecosystem‐scale impacts.
Sound level measurements from audio recordings provide objective distance estimates for distance sampling wildlife populations
Authors: Yip Daniel A. , Knight Elly C. , Haave‐Audet Elène , Wilson Scott J. , Charchuk Connor , Scott Chris D. , Sólymos Péter , Bayne Erin M. 
Published: May 2019

AbstractDistance sampling is widely used to estimate animal population densities by accounting for imperfect detection of individuals with increasing distance from an observer. Distance sampling assumes that distances are measured without error; however, it is often applied to human estimated distances, which are known to be inconsistent, inaccurate, and biased. We present an objective technique for estimating distance to vocalizing individuals that relies on the relative sound level (RSL) of the vocalization extracted from autonomous recording unit (ARU) recordings and show the error is less than human estimated error extracted from a literature case study.RSLpredicted distances can be obtained by manual measurement in sound viewing software, or automatically with automated signal recognition software. We built calibration datasets of Ovenbirds (Seiurus aurocapilla) and Common Nighthawks (Chordeiles minor) recorded at known distances and used regression ofRSLfrom those recordings to predict distance. There was no error bias ofRSLpredicted distances when compared to known distances for Common Nighthawk, minimal error bias for Ovenbird, and error from allRSLpredicted distances was less than human estimated error extracted from the literature. We then simulatedARUpoint count surveys with a known density and estimated that density with distance sampling to test whetherRSLdistance prediction does not violate the assumption that distances are measured without error. There was no difference in density estimates from known distance and density estimates obtained fromRSLpredicted distance, while density estimates contaminated with human estimated error were significantly lower than density estimates from known distance. We found that a calibration dataset of approximately 300 vocalizations was suitable to minimize error for both species, and so conclude thatRSLdistance prediction is an accessible method of improving distance estimates relative to human estimation. We provide general recommendations on how to collect calibration recordings for the application ofRSLdistance prediction to other species and areas.
Study on multistage anodization for high‐modulus carbon fiber
Authors: Fu Yingpiao , Lu Yike , You Ting , Wu Jianglu , Cao Weiyu 
Published: May 2019

Because of the strong chemical inertness of high‐modulus carbon fiber (HMCF), the surface treatment of HMCF becomes less effective. In this work, multistage anodization method was applied to modify HMCF. NH4HCO3 and (NH4)2SO4 were used as electrolytes to study the influence of electrolyte type on the oxidation effect in the multistage oxidation process. The HMCF was treated by multistage anodization in the following three ways: NH4HCO3 → NH4HCO3, (NH4)2SO4 → (NH4)2SO4, and NH4HCO3 → (NH4)2SO4. In order to compare with the multistage anodization methods, HMCF was also anodized by NH4HCO3 and (NH4)2SO4 using single anodization method, respectively. The treated HMCFs were characterized in detail using X‐ray photoelectron spectroscopy (XPS), Raman spectroscopy, Scanning Electron Microscope (SEM), Atomic Force Microscope (AFM), and dynamic contact angle analysis, respectively. The results of XPS exhibited that HMCF modified by multistage anodization showed more functional groups on its surface. More sp2 hybridized bonds were converted into sp3 hybridized bonds after modification by multistage anodization, especially when (NH4)2SO4 was used as electrolyte. The results of SEM/AFM indicated that HMCF modified by multistage showed a greater surface roughness. Interlaminar shear strength (ILSS) was used to observe the interfacial properties of HMCF between epoxy resin. The results showed that ILSS of untreated carbon fiber composite was only 27.3 MPa; it was increased to 71.8 and 78.6 MPa after treated with single anodization by NH4HCO3 and (NH4)2SO4, respectively. After modification by multistage anodization, the ILSS increased in different degrees compared with single anodization method. Especially, the ILSS reached 93.1 MPa when the method of using NH4HCO3 followed by (NH4)2SO4 was used to treat HMCF.
Solution‐Processable Photonic Inks of Mie‐Resonant Hollow Carbon–Silica Nanospheres
Authors: Kim Seung‐Hyun , Hwang Victoria , Lee Sang Goo , Ha Jong‐Wook , Manoharan Vinothan N. , Yi Gi‐Ra 
Published: May 2019

AbstractHollow carbon–silica nanospheres that exhibit angle‐independent structural color with high saturation and minimal absorption are made. Through scattering calculations, it is shown that the structural color arises from Mie resonances that are tuned precisely by varying the thickness of the shells. Since the color does not depend on the spatial arrangement of the particles, the coloration is angle independent and vibrant in powders and liquid suspensions. These properties make hollow carbon–silica nanospheres ideal for applications, and their potential in making flexible, angle‐independent films and 3D printed films is explored.
Characterizing application scheduling on edge, fog, and cloud computing resources
Authors: Varshney Prateeksha , Simmhan Yogesh 
Published: May 2019

SummaryCloud computing has grown to become a popular distributed computing service offered by commercial providers. More recently, edge and fog computing resources have emerged on the wide‐area network as part of Internet of things (IoT) deployments. These three resource abstraction layers are complementary, and offer distinctive benefits. Scheduling applications on clouds has been an active area of research, with workflow and data flow models offering a flexible abstraction to specify applications for execution. However, the application programming and scheduling models for edge and fog are still maturing, and can benefit from learnings on cloud resources. At the same time, there is also value in using these resources cohesively for application execution. In this article, we offer a taxonomy of concepts essential for specifying and solving the problem of scheduling applications on edge, fog, and cloud computing resources. We first characterize the resource capabilities and limitations of these infrastructure and offer a taxonomy of application models, quality‐of‐service constraints and goals, and scheduling techniques, based on a literature review. We also tabulate key research prototypes and papers using this taxonomy. This survey benefits developers and researchers on these distributed resources in designing and categorizing their applications, selecting the relevant computing abstraction(s), and developing or selecting the appropriate scheduling algorithm. It also highlights gaps in literature where open problems remain.
Metabolomic and Transcriptional Analyses Reveal Atmospheric Oxygen During Human Induced Pluripotent Stem Cell Generation Impairs Metabolic Reprogramming
Authors: Spyrou James , Gardner David K. , Harvey Alexandra J. 
Published: May 2019

Abstract
The transition to pluripotency invokes profound metabolic restructuring; however, reprogramming is accompanied by the retention of somatic cell metabolic and epigenetic memory. Modulation of metabolism during reprogramming has been shown to improve reprogramming efficiency, yet it is not known how metabolite availability during reprogramming affects the physiology of resultant induced pluripotent stem cells (iPSCs). Metabolic analyses of iPSCs generated under either physiological (5%; P-iPSC) or atmospheric (20%; A-iPSC) oxygen conditions revealed that they retained aspects of somatic cell metabolic memory and failed to regulate carbohydrate metabolism with A-iPSC acquiring different metabolic characteristics. A-iPSC exhibited a higher mitochondrial membrane potential and were unable to modulate oxidative metabolism in response to oxygen challenge, contrasting with P-iPSC. RNA-seq analysis highlighted that A-iPSC displayed transcriptomic instability and a reduction in telomere length. Consequently, inappropriate modulation of metabolism by atmospheric oxygen during reprogramming significantly impacts the resultant A-iPSC metabolic and transcriptional landscape. Furthermore, retention of partial somatic metabolic memory in P-iPSC derived under physiological oxygen suggests that metabolic reprogramming remains incomplete. As the metabolome is a regulator of the epigenome, these observed perturbations of iPSC metabolism will plausibly have downstream effects on cellular function and physiology, both during and following differentiation, and highlight the need to optimize nutrient availability during the reprogramming process. Stem Cells 2019;37:1042–1056