bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transition paths across the EMT landscape are dictated by network logic
Authors: Dey Anupam , MacLean Adam L. 
Published: December 2024

AbstractDuring development and cancer metastasis, cells transition reversibly from epithelial to mesenchymal via intermediate cell states during epithelial-mesenchymal transition (EMT). EMT is controlled by gene regulatory networks (GRNs) and can be described by a three-node GRN that permits tristable EMT landscapes. In this GRN, multiple inputs regulate the transcription factor ZEB that induces EMT. It is unknown how to choose the network logic for such regulation. Here we explore the effects of network logic on a tristable EMT network. We discover that the choice of additive vs multiplicative logic affects EMT phenotypes, leading to opposing predictions regarding the factors controlling EMT transition paths. We show that strong inhibition of miR-200 destabilizes the epithelial state and initiates EMT for multiplicative (AND) but not additive (OR) logic, suggesting that AND logic is in better agreement with experimental measurements of the effects of miR-200 regulation on EMT. Using experimental single-cell data, stochastic simulations, and perturbation analysis, we demonstrate how our results can be used to design experiments to infer the network logic of an EMT GRN in live cells. Our results explain how the manipulation of molecular interactions can stabilize or destabilize EMT hybrid states, of relevance during cancer progression and metastasis. More generally, we highlight the importance of the choice of network logic in GRN models in the presence of biological noise and multistability.
Structural and functional interrelationship of histone H2A and its variants H2A.Z and H2A.W in Arabidopsis
Authors: Wang Youchao , Wu Jiabing , Yang Shuoming , Li Xiang , Wang Jiachen , Lv Qinghe , Zhu Xiaoyu , Lu Guoliang , Zhang Jinru , Shen Wen-Hui , Liu Bing , Lin Jinzhong , Dong Aiwu 
Published: December 2024

AbstractMultiple histone H2A variants are known in eukaryotes. However, the functional relationship between H2A and its variants in plants remains largely obscure. Using CRISPR/Cas9 editing, we generated a mutant lacking four H2A isoforms in Arabidopsis and analyzed the functional and structural relationship between H2A and its variants H2A.Z and H2A.W. RNA-sequencing and phenotype analyses revealed mild changes in gene transcription and plant development in the mutants lacking H2A, H2A.Z, or H2A.W compared with the wild-type plants. Chromatin immunoprecipitation sequencing analysis showed that H2A is able to substitute for both H2A.Z and H2A.W across the genome, including in euchromatin and heterochromatin regions. However, H2A.Z replaced both H2A and H2A.W primarily within the euchromatin regions. By using DNA and histones derived from Arabidopsis, we constructed nucleosomes containing H2A, H2A.Z, or H2A.W and resolved their cryogenic electron microscopy structures at near-atomic resolution. Collectively, the results reveal the structural similarity and functional redundancy of H2A and H2A variants in Arabidopsis.
Theory of higher education learning
Authors: Awaah Fred 
Published: December 2024

PurposeThis study proposes the theory of higher education learning (THEL). The theory argues that certain concepts are difficult to understand, so learners and instructors should consider common factors that will enhance learners’ understanding of key concepts that are difficult to comprehend across various fields of study. The components of this theory have been tested in three different fields (public administration, entrepreneurship and chemistry) in separate studies in Ghana, Nigeria and Burundi within a five-year span to validate their applicability to higher education learning.Design/methodology/approachThe design covers empirical, philosophical and theoretical discussions and comparative studies of other researchers and the authors’ work and thinking.FindingsThis theory proposes five variables (gender, age, opportunity, instructor knowledge and instructor confidence) that address students’ difficulty in understanding concepts in higher education learning. These factors are premised on six assumptions: (1) gender orientation must be either male or female; (2) age must be consistent with the parameter set; (3) there must be learners’ desire to study the same subject after school; (4) there must be an institution offering further studies on the same subject; (5) the instructor might have understood the subject in his/her official training, experience or practice and (6) the instructor must demonstrate certainty, accuracy, verifiability and reliability in the transfer of knowledge to the learners. Given these assumptions, the uniqueness of this theory lies in its application of the five variables to solve challenges uniquely related to studies in higher education.Originality/valueThis theory will incite debate and provide further insights into higher education learning models. The novelty lies in the five prepositions proven to enhance effective teaching and learning in higher education. Specifically, it introduces an extension to Piaget’s cognitive constructivist theory by proposing higher age brackets for students at the university level, opportunity (for future studies) to close the gap in Ausubul’s theory of advance organizers and endogenous factors to bridge the gap in Okebukola’s culturo-techno-contextual approach.
Determinants of higher education graduates’ employability: a scoping review
Authors: Monteiro Sílvia , Santos Sandra , Teixeira José Nuno , Torres Leonor , Palhares José 
Published: December 2024

PurposeGraduates’ employability has been recognised as a complex and multidimensional phenomenon in the theoretical literature. However, some critics have emerged around the lack of relation between structural, contextual and individual dimensions that have been acknowledged as relevant for the understanding of graduates’ employability. This study aims to map empirical research, grounded on a scoping and integrative review of the research published to date on the determinants of graduates’ employability and offer a wider perspective of the knowledge produced across different disciplinary fields, and theoretical and methodological approaches.Design/methodology/approachFollowing the Preferred Reporting Items for Systematic Reviews and Meta-Analyses protocol for Scoping Reviews, a systematic and extensive search of the literature was carried out and 74 studies focusing on the determinants of graduates’ employability were selected through inclusion and exclusion criteria. Then, data were extracted, analysed and interpreted with quantitative and qualitative techniques.FindingsThe results evidence that multiple factors mainly related to higher education attributes, educational arrangement, personal and interpersonal attributes, and socioeconomic conditions predict the odds of obtaining a job after graduation. This review also demonstrates some fragmentation of knowledge produced in this field and identifies a lack of an integrative approach, calling for more multidisciplinary research that might favour a more comprehensive knowledge of graduates’ employability.Originality/valueBy considering the different factors together, from individual and contextual levels, the present review offers an original and clearer overall picture of what is currently known and what is still unresearched about the determinants of graduates’ employability.
Two-phase analysis of blood in microchannel architecture on plasma separation ability with dimensional variance
Authors: Khaliq Amin , Lee Byungkwon , Kamran Muhammad Ahmad , Jeong Myung Yung 
Published: December 2024

Purpose
This paper aims to study the effects of varying inlet channel angle in a novel microfluidic architecture blood plasma separation ability over range of hematocrit values (5–45%) at multiple flowrates.

Design/methodology/approach
CAD designs for both micro architectures were designed in SOILWORKS. In the second step, these designs were imported into ANSYS to perform where meshing, model selection, defining blood as two-phase material and boundary conditions are performed.

Findings
Separation efficiency values close to 100% with diluted blood and 65.2% with whole blood were observed. Straight channel inlet design has significantly better performance at high hematocrit levels, whereas at lower hematocrit levels, both designs had almost same outcome. Furthermore, lower flowrates have shown the highest separation efficiency for lower hematocrit levels, whereas at higher hematocrit percentages, higher flowrates have shown better separation effects for both designs. Furthermore, trends obtained for flow ratio and flowrates against separation efficiency are demonstrated.

Research limitations/implications
This study is based on blood modeled as two-phase flow, with the phases consisting of blood plasma as primary phase and red blood cells as secondary particulate phase.

Practical implications
Implications of this study are far reaching for point-of-care health-care systems. A practical system of this numerical study can provide a microchannel device which take very small amount of blood sample to separate it into constituents which can be coupled with detection module to detect a particular disease for which it is designed for. This microsystem can be very beneficial for remote areas where a large hospital facility is far away.

Originality/value
This study has carried out a detailed analysis on the ability of a novel microchannel architecture to separate blood plasma from other blood constituents. Inlet channel angle variation effects are observed over a range of hematocrit percentages. These trends are further investigated for three different flowrates to assess the microchannel design behavior.
Simulation and theoretical studies on the influence of removable panel deformation on the seal characteristics of ceramic wafer seal
Authors: Wang Qiliang , Shao Xulong , Wang Dagang , Wei Shijun , Kang Jinyuan , Wang Jun 
Published: December 2024

Purpose
The purpose of this study is to investigate the influence of geometric parameters of removable panels on the sealing characteristics of ceramic wafer seal structure subjected to high-temperature gas flow.

Design/methodology/approach
Based on the laminar flow Reynolds equation, the theoretical and numerical calculation models were constructed to investigate the influence of external convex deformation of removable panel on leakage rate. The theoretical formula for leakage rate after deformation of the removable panel was derived, and the flow field and leakage characteristics of ceramic wafer seal under different operating parameters were studied.

Findings
The leakage rate exhibits consistent trends between theoretical calculation, numerical simulation and experimental value, with a maximum discrepancy of 8.9%. This validates the accuracy of both the theoretical model and numerical simulation. As the deformation angle of the removable panel increases, the sealing gap gradually widens, resulting in a compromised sealing effect. Moreover, the leakage rate in the central region of the sealing area is lower compared to that at both ends.

Originality/value
The leakage of the ceramic wafer seal structure under the removable panel with different deformation angles can be monitored based on Reynolds equation. The pseudo-transient numerical calculation method can be used to determine the leakage value of the micro-state ceramic wafer seal structure. These research findings provide a theoretical foundation and numerical investigation approach for studying ceramic wafer seal structures.
A CAM-UNet for pose determination of supercapacitor cell module assembly based on monocular vision
Authors: Wu Zhichao , Shu Weijing , Song Limei , Zhu Xinjun , Yang Yangang 
Published: December 2024

Purpose
This paper aims to solve the problems of low stacking efficiency and long production time in the supercapacitor module assembly process, a stacking system based on monocular vision is proposed, including bracket visual positioning, grasping and stacking, and it is applied in actual production.

Design/methodology/approach
To enhance the robustness of the workpiece location method and improve the location accuracy, the improved U-Net network and image processing algorithms are used to segment the collected images. In addition, for the extracted feature points, the objective function that can be globally optimized is obtained by parameterizing the rotation matrix to construct a polynomial equation system and, finally, the equation system is solved to obtain the final pose estimation, which could improve the accuracy of workpiece location.

Findings
The result indicates that the proposed method is successfully performed on the manipulator. Besides, this method can well solve the problem of object reflection on the conveyor belt. The Intersection over Union of the image segmentation of the object is 0.9948, and the Pixel Accuracy is 0.9973, which has a high segmentation accuracy for the image. The error range between the method proposed in this paper and the pose estimation is within 2 mm, and the qualified rate of supercapacitor module stacking products is over 99.8%.

Originality/value
This paper proposes a method of accurately extracting feature points by integrating an improved U-Net network and image processing and uses the workpiece positioning algorithm of the optimal solution PnP problem algorithm. The calculation results show that the algorithm improves the positioning accuracy of the workpiece, realizes the assembly of stacked supercapacitor modules and is applied in industrial production.
Expert consensus on serratus anterior plane block education and credentialing: A modified‐Delphi study
Authors: Partyka Christopher , Gaetani Daniel , Delaney Anthony , Curtis Kate 
Published: December 2024

AbstractObjectiveThe serratus anterior plane block (SAPB) is a regional anaesthesia technique with increasing use as an analgesic adjunct in patients with rib fractures. The present study aimed to generate consensus of the requirements of education, training and credentialing for the use of a ‘single shot’ SAPB in the management of rib fractures.MethodsA modified Delphi process was designed using online questionnaires. Expert panellists from Australian and Aotearoa New Zealand were invited from the fields of Emergency Medicine and Anaesthesia and were asked to rate the importance of different components of SAPB education, training and credentialling on a 9‐point Likert scale. Consensus was achieved if ≥70% of experts provided a score of seven or greater on this scale for any given statement.ResultsThirty specialists (60% FACEM, 40% FANZCA) representing New Zealand plus all states and territories of Australia formed the expert panel. Participant response rates were 100% (first round), 83% (second round) and 63% (final round). At the end of three survey rounds, 59 consensus statements were formed (27 for education, 5 for training and 17 for credentialing).ConclusionsThis series of expert statements provides consensus on the education, training and credentialling of the SAPB for the management of rib fractures. These serve as the minimum standard by which this procedure should be taught while providing clinicians with a syllabus for the development of training programmes.
Humble Leadership and Employee Feedback Seeking: The Role of Affective Trust in the Leader and Perceived Human Resource Management Strength
Authors: Jia Jianfeng , Zheng Yuyan , Jiao Yuxin 
Published: December 2024

ABSTRACTDespite the burgeoning literature on leadership and employees' feedback‐seeking behaviors, research linking contemporary leadership styles and feedback seeking is still limited. Our study examines the role of humble leadership on employees' feedback‐seeking behaviors. Based on three‐wave data from 172 leader‐follower dyadic pairs, our results show that humble leadership has a positive effect on feedback‐seeking behaviors via employees' affective trust in the leader when the mediating effects of psychological safety and cognitive trust were controlled. Furthermore, the overall mediation effect of affective trust is strengthened by higher levels of employees' perceived human resource management strength. Theoretical and practical implications are discussed.
Characteristic volatile compounds of white tea with different storage times using E‐nose, HS–GC–IMS, and HS–SPME–GC–MS
Authors: Huang Haoran , Chen Xinyu , Wang Ying , Cheng Ye , Liu Zhijian , Hu Yunchao , Wu Xianzhi , Wu Caie , Xiong Zhixin 
Published: December 2024

AbstractThis paper studied the influence of storage duration on the flavor profile of white tea in detail, with samples produced between 2020 and 2023. Sensory evaluation was performed by quantitative descriptive analysis (QDA), followed by an in‐depth aroma components analysis employing an electronic nose (E‐nose), headspace gas chromatography–ion mobility spectrometry (HS–GC–IMS), and headspace solid–phase microextraction–gas chromatography–mass spectrometry (HS–SPME–GC–MS). The QDA findings revealed a gradual transition in the flavor profile of white tea during storage, shifting from sweet, fruity, and floral to more herbal and stale characteristics. E‐nose could well distinguish white tea with different storage times. A total of 55 and 53 volatile compounds were identified by HS–GC–IMS and HS–SPME–GC–MS, respectively. The orthogonal partial least squares‐discriminant analysis models, based on HS–GC–IMS (R2Y = 0.998, Q2 = 0.987) and HS–SPME–GC–MS (R2Y = 0.984, Q2 = 0.993), successfully distinguished white tea samples stored for different storage times. Furthermore, 14 and 8 key compounds were screened based on the double variable criterion of one‐way analysis of variance (p < 0.05) and variable importance in projection (VIP) >1.2, and their content changes were also compared. It is the gradual decrease of important aroma components such as 2‐hexenal, 2‐methyl‐2‐hepten‐6‐one, linalool, and geraniol, which are positively correlated with sweet, fruity, and floral aromas, and the gradual increase of hexanoic acid, thiophene, propanoic acid, dimethyl disulfide, and borneyl acetate, which are positively correlated with herbal and stale flavor, that leads to the changes in flavor and aroma of white tea during storage. The results of the study provided a reference for elucidating the aroma characteristics of white tea at different storage times as well as a theoretical basis for the quality control of white tea.
Bone Erosions and Systemic Bone Loss on HR‐pQCT in Men With Tophaceous Gout
Authors: Rocha Gabriela F. , Santos Jeane B. , Sales Lucas P. , Franco André S. , Caparbo Valéria F. , Takayama Liliam , Domiciano Diogo S. , Fuller Ricardo , Figueiredo Camille P. 
Published: December 2024

ABSTRACTAimsGout, a prevalent inflammatory arthropathy, predominantly affects males and arises from persistent hyperuricemia, resulting in monosodium urate crystal deposition. Hyperuricemia is associated with comorbidities, exacerbating patient morbidity. Conflicting literature exists regarding uric acid's impact on bone mineral density (BMD), with potential proinflammatory effects in gout patients. Localized bone destruction (erosions) is a hallmark of gout, necessitating early detection due to its predictive role in musculoskeletal disability.MethodsThis cross‐sectional study included 26 tophaceous gout patients. Clinical and densitometric parameters were assessed, and high‐resolution peripheral quantitative computed tomography (HR‐pQCT) was used for bone microarchitecture evaluation, as well as bone erosions in metacarpophalangeal (MCP) and proximal interphalangeal (PIP) joints. A healthy control group of 52 age and BMI‐matched individuals was included.ResultsDespite normal areal bone mineral density (BMD), tophaceous gout patients exhibited impaired HR‐pQCT parameters, including lower cortical volumetric BMD (Ct.vBMD) and higher cortical porosity at the distal radius. Similar trends were observed at the tibia. Bone erosions were prevalent (96%), with distribution across MCP and PIP joints. Patients with ≥ 4 erosions displayed increased tophi prevalence and longer uricosuric use. Erosions correlated with compromised microarchitecture, emphasizing their association with disease activity.ConclusionDespite normal BMD, tophaceous gout patients manifest systemic bone loss, with bone microarchitectural deterioration and localized bone erosions, underscoring the need for detailed clinical approaches to prevent musculoskeletal disabilities, including fragility fractures, in this population.
Estimating industry conduct using promotion data
Authors: Michel Christian , Paz y Miño Jose Manuel , Weiergraeber Stefan 
Published: December 2024

AbstractWe estimate the evolution of competition in the ready‐to‐eat cereal industry. To separately identify detailed patterns of industry conduct from unobserved marginal cost shocks, we construct novel instruments that interact data on rival firms' promotions with measures of products' relative isolation in the characteristics space. We find strong evidence for partial price coordination among cereal manufacturers in the beginning of our sample. After a merger in 1993 conduct becomes more competitive and on average consistent with multiproduct Nash pricing. The last part of our sample is characterized by even more aggressive pricing, implying median wholesale margins of less than 5%.
Skeletal muscle mitochondrial fragmentation predicts age‐associated decline in physical capacity
Authors: Goulding Richie P. , Charlton Braeden T. , Breedveld Ellen A. , van der Laan Matthijs , Strating Anne R. , Noort Wendy , Kolodyazhna Aryna , Appelman Brent , van Vugt Michèle , Grootemaat Anita E. , van der Wel Nicole N. , de Koning Jos J. , Bloemers Frank W. , Wüst Rob C. I. 
Published: December 2024

AbstractAgeing substantially impairs skeletal muscle metabolic and physical function. Skeletal muscle mitochondrial health is also impaired with ageing, but the role of skeletal muscle mitochondrial fragmentation in age‐related functional decline remains imprecisely characterized. Here, using a cross‐sectional study design, we performed a detailed comparison of skeletal muscle mitochondrial characteristics in relation to in vivo markers of exercise capacity between young and middle‐aged individuals. Despite similar overall oxidative phosphorylation capacity (young: 99 ± 17 vs. middle‐aged: 99 ± 27 pmol O2.s−1.mg−1, p = 0.95) and intermyofibrillar mitochondrial density (young: 5.86 ± 0.57 vs. middle‐aged: 5.68 ± 1.48%, p = 0.25), older participants displayed a more fragmented intermyofibrillar mitochondrial network (young: 1.15 ± 0.17 vs. middle‐aged: 1.55 ± 0.15 A.U., p < 0.0001), a lower mitochondrial cristae density (young: 23.40 ± 7.12 vs. middle‐aged: 13.55 ± 4.10%, p = 0.002) and a reduced subsarcolemmal mitochondrial density (young: 22.39 ± 6.50 vs. middle‐aged: 13.92 ± 4.95%, p = 0.005). Linear regression analysis showed that 87% of the variance associated with maximal oxygen uptake could be explained by skeletal muscle mitochondrial fragmentation and cristae density alone, whereas subsarcolemmal mitochondrial density was positively associated with the capacity for oxygen extraction during exercise. Intramuscular lipid accumulation was positively associated with mitochondrial fragmentation and negatively associated with cristae density. Collectively, our work highlights the critical role of skeletal muscle mitochondria in age‐associated declines in physical function.