bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retracted: Protective impacts of long noncoding RNA taurine‐upregulated 1 against lipopolysaccharide‐evoked injury in MRC‐5 cells through inhibition of microRNA‐127
Authors: Meng Jie , Chen Yan , Zhang Cunxue 
Published: April 2019

AbstractBackgroundPneumonia is a respiratory disease, which is triggered by pathogenic microorganisms or physical/chemical factors. Increasing evidence confirmed the vital impacts of long noncoding RNAs on various inflammatory diseases. Nonetheless, the influence of taurine‐upregulated 1 (TUG1) in pneumonia remains vague. The research tried to disclose the protective impacts of TUG1 against lipopolysaccharide (LPS)‐evoked injury in MRC‐5 cells.MethodsMRC‐5 cells were disposed with LPS to construct pulmonary injury model. Then, pc‐TUG1 vector was transfected into MRC‐5 cells and the influence of overexpressed TUG1 in cell viability, apoptosis, and pro‐inflammatory cytokines in LPS‐disposed cells were evaluated. The correlation between TUG1 and microRNA (miR)−127 was estimated via utilizing real‐time quantitative polymerase chain reaction (RT‐qPCR), meanwhile whether miR‐127 affected the impacts of TUG1 on LPS‐injured MRC‐5 cells was explored. Besides, NF‐κB and p38MAPK pathways were evaluated to understand the dormant mechanisms.ResultsLPS administration apparently evoked inflammatory injury in MRC‐5 cells by restraining cell viability, accelerating apoptosis, and enhancing TNF‐α and IL‐6 productions. But, TUG1 lightened LPS‐evoked pro‐inflammatory response in MRC‐5 cells. In addition, miR‐127 was repressed by overexpressed TUG1, meanwhile the protective impacts of TUG1 against LPS‐evoked inflammatory injury in MRC‐5 cells were overturned by overexpressed miR‐127. Finally, we disclosed that TUG1 hindered the activation of NF‐κB and p38MAPK pathways via restraining miR‐127.ConclusionsThese explorations testified that taurine‐upregulated 1 (TUG1) protected MRC‐5 cells against lipopolysaccharide (LPS)‐evoked inflammatory injury via hindering miR‐127/NF‐κB/p38MAPK axis.
Significant changes in hepatic transcriptome and circulating miRNAs are associated with diet‐induced metabolic syndrome in apoE3L.CETP mice
Authors: Nasias Dimitris , Evangelakos Ioannis , Nidris Vasilis , Vassou Despoina , Tarasco Erika , Lutz Thomas A. , Kardassis Dimitris 
Published: April 2019

AbstractLong‐term exposure to excess dietary fat leads to obesity and the metabolic syndrome (MetS). The purpose of the present study was to identify global changes in liver gene expression and circulating miRNAs in a humanized mouse model of diet‐induced MetS. Male apoE3L.CETP mice received a high‐fat diet (HFD) or a low‐fat diet (LFD) for different time periods and the progression of MetS pathology was monitored. A separate group of mice was divided into responders (R) or nonresponders (NR) and received HFD for 16 weeks. We found that mice receiving the HFD developed manifestations of MetS and displayed an increasing number of differentially expressed transcripts at 4, 8, and 12 weeks compared with mice receiving the LFD. Significantly changed genes were functionally annotated to metabolic diseases and pathway analysis revealed the downregulation of genes in cholesterol and fatty acid biosynthesis and upregulation of genes related to lipid droplet formation, which was in line with the development of hepatic steatosis. In the serum of the apoE3L.CETP mice we identified three miRNAs that were upregulated specifically in the HFD group. We found that responder mice have a distinct gene signature that differentiates them from nonresponders. Comparison of the two diet intervention studies revealed a limited number of common differentially expressed genes but the expression of these common genes was affected in a similar way in both studies. In conclusion, the characteristic hepatic gene signatures and serum miRNAs identified in the present study provide novel insights to MetS pathology and could be exploited for diagnostic or therapeutic purposes.
IL‐6 counteracts the inhibitory effect of IL‐4 on osteogenic differentiation of human adipose stem cells
Authors: Bastidas‐Coral Angela P. , Hogervorst Jolanda M. A. , Forouzanfar Tymour , Kleverlaan Cornelis J. , Koolwijk Pieter , Klein‐Nulend Jenneke , Bakker Astrid D. 
Published: April 2019

AbstractFracture repair is characterized by cytokine production and hypoxia. To better predict cytokine modulation of mesenchymal stem cell (MSC)‐aided bone healing, we investigated whether interleukin 4 (IL‐4), IL‐6, and their combination, affect osteogenic differentiation, vascular endothelial growth factor (VEGF) production, and/or mammalian target of rapamycin complex 1 (mTORC1) activation by MSCs under normoxia or hypoxia. Human adipose stem cells (hASCs) were cultured with IL‐4, IL‐6, or their combination for 3 days under normoxia (20% O
2) or hypoxia (1% O
2), followed by 11 days without cytokines under normoxia or hypoxia. Hypoxia did not alter IL‐4 or IL‐6‐modulated gene or protein expression by hASCs. IL‐4 alone decreased runt‐related transcription factor 2 (RUNX2) and collagen type 1 (COL1) gene expression, alkaline phosphatase (ALP) activity, and VEGF protein production by hASCs under normoxia and hypoxia, and decreased mineralization of hASCs under hypoxia. In contrast, IL‐6 increased mineralization of hASCs under normoxia, and enhanced RUNX2 gene expression under normoxia and hypoxia. Neither IL‐4 nor IL‐6 affected phosphorylation of the mTORC1 effector protein P70S6K. IL‐4 combined with IL‐6 diminished the inhibitory effect of IL‐4 on ALP activity, bone nodule formation, and VEGF production, and decreased RUNX2 and COL1 expression, similar to IL‐4 alone, under normoxia and hypoxia. In conclusion, IL‐4 alone, but not in combination with IL‐6, inhibits osteogenic differentiation and angiogenic stimulation potential of hASCs under normoxia and hypoxia, likely through pathways other than mTORC1. These results indicate that cytokines may differentially affect bone healing and regeneration when applied in isolation or in combination.
Outcomes of pancreaticoduodenectomy in patients with chronic hepatic dysfunction including liver cirrhosis: results of a retrospective multicenter study by the Japanese Society of Hepato‐Biliary‐Pancreatic Surgery
Authors: Futagawa Yasuro , Yanaga Katsuhiko , Kosuge Tomoo , Suka Machi , Isaji Shuji , Hirano Satoshi , Murakami Yoshiaki , Yamamoto Masakazu , Yamaue Hiroki 
Published: April 2019

AbstractBackgroundSince there is no reliable evidence on the safety of pancreaticoduodenectomy (PD) in chronic hepatic dysfunction (CHD) including liver cirrhosis (LC), the effects of CHD on patients undergoing PD were investigated.MethodsThis multi‐institutional retrospective study analyzed 529 patients with CHD, including 105 patients diagnosed with LC, who underwent PD at 82 high‐volume institutions between 2004 and 2013.ResultsThe in‐hospital mortality rate was 5.9%. The incidence of postoperative hepatic decompensation upon discharge and refractory ascites was 10.2% and 8.9%, respectively. For hepatic decompensation, the serum aspartate aminotransferase (AST) of more than 50 IU/l and portal hypertension (PHT) were independent significant risk factors. For refractory ascites, prothrombin activity of <70%, serum AST of more than 50 IU/l and advanced PHT with collaterals were significant risk factors. Five‐year overall survival was 57.8% in Child A and 24.8% in Child B patients (P < 0.0001). The Child B/C patients were divided into two groups according to an AST‐platelet ratio index (APRI) of 1.0; the APRI of <1.0 yielded a significantly higher survival rate than their counterpart (43.2% vs. 14.7%, P = 0.04).ConclusionsIn addition to PHT, pre‐operative evaluation of AST and APRI may be helpful for patient selection for PD in patients with CHD.
Development and radiosynthesis of the first 18F‐labeled inhibitor of monocarboxylate transporters (MCTs)
Authors: Sadeghzadeh Masoud , Moldovan Rareş‐Petru , Fischer Steffen , Wenzel Barbara , Ludwig Friedrich‐Alexander , Teodoro Rodrigo , Deuther‐Conrad Winnie , Jonnalagadda Shirisha , Jonnalagadda Sravan K. , Gudelis Emilis , Šačkus Algirdas , Higuchi Kei , Ganapathy Vadivel , Mereddy Venkatram R. , Drewes Lester R. , Brust Peter 
Published: April 2019

Monocarboxylate transporters 1 and 4 (MCT1 and MCT4) are involved in tumor development and progression. Their expression levels are related to clinical disease prognosis. Accordingly, both MCTs are promising drug targets for treatment of a variety of human cancers. The noninvasive imaging of these MCTs in cancers is regarded to be advantageous for assessing MCT‐mediated effects on chemotherapy and radiosensitization using specific MCT inhibitors. Herein, we describe a method for the radiosynthesis of [18F]FACH ((E)‐2‐cyano‐3‐{4‐[(3‐[18F]fluoropropyl)(propyl)amino]‐2‐methoxyphenyl}acrylic acid), as a novel radiolabeled MCT1/4 inhibitor for imaging with PET. A fluorinated analog of α‐cyano‐4‐hydroxycinnamic acid (FACH) was synthesized, and the inhibition of MCT1 and MCT4 was measured via an L‐[14C]lactate uptake assay. Radiolabeling was performed by a two‐step protocol comprising the radiosynthesis of the intermediate (E)/(Z)‐[18F]tert‐Bu‐FACH (tert‐butyl (E)/(Z)‐2‐cyano‐3‐{4‐[(3‐[18F]fluoropropyl)(propyl)amino]‐2‐methoxyphenyl}acrylate) followed by deprotection of the tert‐butyl group. The radiofluorination was successfully implemented using either K[18F]F‐K2.2.2‐carbonate or [18F]TBAF. The final deprotected product [18F]FACH was only obtained when [18F]tert‐Bu‐FACH was formed by the latter procedure. After optimization of the deprotection reaction, [18F]FACH was obtained in high radiochemical yields (39.6 ± 8.3%, end of bombardment (EOB) and radiochemical purity (greater than 98%).
An approach for visualizing the spatial metabolome of an entire plant root system inspired by the Swiss‐rolling technique
Authors: Veličković Dušan , Chu Rosalie K. , Myers Gabriel L. , Ahkami Amir H. , Anderton Christopher R. 
Published: April 2019

AbstractThe spatial configuration and morphology of roots are commonly monitored for a better understanding of plant health and development. However, this approach provides minimal details about the biochemistry regulating the observable traits. Therefore, the ability to metabolically map the entire root structure would be of major value. Here, we developed a sample preparation approach that enables imaging of the entire root within a restricted space (width of microscope slide), which was influenced by the Swiss‐rolling technique. We were able to image and confidently identify molecules along the entire root structure from rolled‐root tissue sections using multiple spatially resolved mass spectrometry approaches.
Viruses associated with acute respiratory infection in a community‐based cohort of healthy New Zealand children
Authors: Walker Gregory J. , Stelzer‐Braid Sacha , Shorter Caroline , Honeywill Claire , Wynn Matthew , Willenborg Christiana , Barnes Phillipa , Kang Janice , Pierse Nevil , Crane Julian , Howden‐Chapman Philippa , Rawlinson William D﻿.﻿ 
Published: April 2019

AbstractAcute respiratory infections (ARIs) are a major cause of morbidity among children. Respiratory viruses are commonly detected in both symptomatic and asymptomatic periods. The rates of infection and community epidemiology of respiratory viruses in healthy children needs further definition to assist interpretation of molecular diagnostic assays in this population. Children otherwise healthy aged 1 to 8 years were prospectively enrolled in the study during two consecutive winters, when ARIs peak in New Zealand. Parents completed a daily symptom diary for 8 weeks, during which time they collected a nasal swab from the child for each clinical ARI episode. A further nasal swab was collected by research staff during a clinic visit at the conclusion of the study. All samples were tested for 15 respiratory viruses commonly causing ARI using molecular multiplex polymerase chain reaction assays. There were 575 ARIs identified from 301 children completing the study, at a rate of 1.04 per child‐month. Swabs collected during an ARI were positive for a respiratory virus in 76.8% (307 of 400), compared with 37.3% (79 of 212) of swabs collected during asymptomatic periods. The most common viruses detected were human rhinovirus, coronavirus, parainfluenza viruses, influenzavirus, respiratory syncytial virus, and human metapneumovirus. All of these were significantly more likely to be detected during ARIs than asymptomatic periods. Parent‐administered surveillance is a useful mechanism for understanding infectious disease in healthy children in the community. Interpretation of molecular diagnostic assays for viruses must be informed by understanding of local rates of asymptomatic infection by such viruses.
Stage I‐III colon cancer patients with tumor deposits behave similarly to stage IV patients. Cross‐section analysis of 392 patients
Authors: Lino‐Silva Leonardo S. , Anchondo‐Núñez Patricia , Chit‐Huerta Alonso , Aguilar‐Romero Estefanía , Morales‐Soto Jonathan , Salazar‐García Jenny A. , Guzmán‐López Cristina J. , Maldonado‐Martínez Héctor A. , Meneses‐García Abelardo , Salcedo‐Hernández Rosa A. 
Published: April 2019

AbstractBackground and ObjectivesTumor deposits (TDs) are associated with adverse prognostic factors and decreased survival in colorectal cancer. However, controversy exists regarding their definition, evaluation, and staging categories. This study aimed to determine the survival and recurrence impact of the TD in colon adenocarcinomas; and to determine if TD patients behave similarly to stage IV patients.MethodsCross‐section study from 392 patients with colon adenocarcinoma from 2005 to 2012. We performed survival analysis and further stratified patients considering TD patients as a “stage IV‐TD” to demonstrate if they behave similarly than stage IV patients.ResultsFrom 392 patients, 204 (52%) were men, the mean age was 57.4 ± 13.9 years and 11.5% of cases had TD. In a multivariate analysis, TD failed to predict mortality and recurrence. Considering cases with TD as stage IV‐TD, their mean survival was similar to stage IV patients (69.3 and 64.6 months, respectively) and different to those in stage III (110.5 months), II (135.7 months), and I (114.9 months) (P < 0.001).ConclusionsTD failed to predict mortality and recurrence. Patients with TD in stage I‐III shows similar mortality than stage IV patients; then, we suggest putting them into a substage IV category instead of the N1c category.
Profiling the constituents of Dachuanxiong decoction by liquid chromatography with high‐resolution tandem mass spectrometry using target and nontarget data mining
Authors: Liu Jie , Wang Mingxia , Chen Lianming , Li Yueting , Chen Yijun , Wei Ziyi , Jia Zhixin , Xu Wenjuan , Xiao Hongbin 
Published: April 2019

AbstractComprehensive characterization of the large number of compounds existing in traditional Chinese medicines is still a great challenge. In this study, a strategy of precursor ion selected acquisition coupled with target and nontarget data mining was established to systematically characterize the chemical constituents of traditional Chinese medicines. This strategy consisted of four steps: (1) precursor ion selected acquisition was developed to trigger additional tandem mass spectrometry fragmentation reactions, especially for trace constituents; (2) in‐house database of compounds was established and diagnostic characteristics were summarized; (3) compounds were identified by target and nontarget data mining; and (4) compound structures were elucidated based on accurate mass matching and comparison of fragment ions, and isomers were discriminated by the intensity of fragment ions, fragmentation pattern analysis, and calculated log P values. This strategy was successfully applied to comprehensively identify the constituents in Dachuanxiong decoction. Finally, a total of 218 compounds assigned to six categories were characterized, and 107 compounds were characterized by nontarget analysis for the first time. In addition, three new diagnostic characteristics of esters of citric acids were elucidated. This research enriched the material basis of Dachuanxiong decoction and provided a new strategy for identifying the chemical constituents of other traditional Chinese medicines.
A conflict resolution model for sustainable heritage tourism
Authors: Zhang Yang , Lee Timothy J. , Xiong Yu 
Published: April 2019

AbstractThis paper uses Ajzen's theory of planned behavior (TPB) model to explain the formation of behavioral frameworks for conflict resolution. Using data about the Chengqi Earth building, UNESCO World Heritage Site, it puts forward an integrative TPB model with self‐regulation and social capital as a good fit to that data, which has better predictive power in relation to intentions than the original model. The results show that attitudes, subjective norms, perceived behavioral control, and self‐regulatory behavior positively affect intentions to seek conflict resolution. The paper concludes with insightful implications that can be applied to other areas of China and beyond.
Development of Ester Free Type Poly(trimethylene carbonate) Derivatives with Pendant Fluoroaromatic Groups
Authors: Nobuoka Hiroaki , Ajiro Hiroharu 
Published: April 2019

AbstractThe facile method for the synthesis of ester free type poly(trimethylene carbonate) (PTMC) derivatives is previously reported. In this paper, a series of PTMC derivatives with fluoroaromatic groups in the side chain are synthesized to improve polymer properties. Thermal analysis reveals that all the PTMC derivatives have a decomposition temperature exceeding 300 °C. Furthermore, as the number of fluorine attached to the aromatic ring increases, the glass transition temperature decreases. In order to clarify the surface properties of their films, the contact angles and protein adsorption are evaluated. The hydrophobic nature is attributed to the fluorine groups at the side chain. Furthermore, it is suggested that as the number of fluorine attached to the aromatic ring increases, the protein adsorption is suspended. There is a possibility that a new biomaterial could be created by introducing a fluoroaromatic group into the side chain of PTMC with ester free structure.
Impact of introduction of noninvasive prenatal testing on uptake of genetic testing in fetuses with central nervous system anomalies
Authors: Al Toukhi Samar , Chitayat David , Keunen Johannes , Roifman Maian , Seaward Gareth , Windrim Rory , Ryan Greg , Van Mieghem Tim 
Published: April 2019

AbstractObjectiveTo evaluate the impact of introduction of noninvasive prenatal testing (NIPT) on the uptake of invasive testing in pregnancies complicated by fetal central nervous system (CNS) anomalies.MethodsRetrospective review of all singleton pregnancies complicated by fetal CNS anomalies seen at a single tertiary center between 2010 and 2017. Cases who had undergone invasive testing or NIPT prior to the diagnosis of the CNS anomaly were excluded. Cases were segregated according to whether they were seen prior to introduction of NIPT (group A, 2010‐2013) or thereafter (group B, 2014‐2017). We examined the rate of invasive and noninvasive genetic testing in each group.ResultsWe retrieved 500 cases: 308 (62%) were isolated CNS anomalies, and 192 (38%) had additional structural anomalies. In the total cohort, 165 women (33%) underwent expectant management with no further prenatal genetic testing, 166 (33%) had invasive testing, 52 (10%) had NIPT, and 117 pregnancies (23%) were terminated without further prenatal investigations. The introduction of NIPT significantly decreased the number of pregnancies having no testing (44% group A vs 22% in group B, p < .0001), particularly in the group presenting with isolated ventriculomegaly, but did not affect the uptake of invasive testing (34% vs 32%, respectively; p = .61). NIPT would have missed 4% of pathogenic copy number variants (CNVs) in the group of cases with isolated brain anomalies and 11% of CNVs in cases with complex anomalies.ConclusionsUptake of invasive prenatal testing in fetuses with brain anomalies was not affected by NIPT. However, the incidence of no genetic testing was significantly reduced. NIPT was a suboptimal testing strategy in this population as it missed a significant number of subchromosomal genetic anomalies.
Promoting academic satisfaction and performance: Building academic resilience through coping strategies
Authors: Meneghel Isabella , Martínez Isabel M. , Salanova Marisa , de Witte Hans 
Published: April 2019

AbstractThe aim of this article is twofold: (a) to validate the psychometric properties of the Academic Resilience Scale (AR‐S) in a Spanish university context; and (b) to test a model where different coping strategies are antecedents of AR, and where academic satisfaction and performance are its consequences. The studies were conducted with 185 (study 1) and 780 (study 2) students. In study 1, the construct validity, reliability, and convergent and divergent validity of the AR‐S were analyzed. The hypothesized model (study 2) was tested using structural equation modeling. The results confirmed the good psychometric properties of the AR‐S in the Spanish context, providing evidence of its validity. Furthermore, the distinctive role of different coping strategies in resilience was confirmed, as well as the relationship between resilience and academic satisfaction. No direct relationship between resilience and performance was found, highlighting the full mediation of satisfaction.
17O‐excess as a detector for co‐extracted organics in vapor analyses of plant isotope signatures
Authors: Nehemy Magali F. , Millar Cody , Janzen Kim , Gaj Marcel , Pratt Dyan L. , Laroque Colin P. , McDonnell Jeffrey J. 
Published: April 2019

RationaleThe stable isotope compositions of hydrogen and oxygen in water (δ2H and δ18O values) have been widely used to investigate plant water sources, but traditional isotopic measurements of plant waters are expensive and labor intensive. Recent work with direct vapor equilibration (DVE) on laser spectroscopy has shown potential to side step limitations imposed by traditional methods. Here, we evaluate DVE analysis of plants with a focus on spectral contamination introduced by organic compounds. We present17O‐excess as a way of quantifying organic compound interference in DVE.MethodsWe performed isotopic analysis using the δ2H, δ18O and δ17O values of water on an Off‐Axis Integrated Cavity Output Spectroscopy (IWA‐45EP OA‐ICOS) instrument in vapor mode. We used a set of methanol (MeOH) and ethanol (EtOH) solutions to assess errors in isotope measurements. We evaluated how organic compounds affect the17O‐excess. DVE was used to measure the isotopic signatures in natural plant material fromPinus banksiana,Picea mariana, andLarix laricina, and soil from boreal forest for comparison with solutions.ResultsThe17O‐excess was sensitive to the presence of organic compounds in water.17O‐excess changed proportionally to the concentration of MeOH per volume of water, resulting in positive values, while EtOH solutions resulted in smaller changes in the17O‐excess. Soil samples did not show any spectral contamination. Plant samples were spectrally contaminated on the narrow‐band and were enriched in1H and16O compared with source water.L. laricinawas the only species that did not show any evidence of spectral contamination. Xylem samples that were spectrally contaminated had positive17O‐excess values.Conclusions17O‐excess can be a useful tool to identify spectral contamination and improve DVE plant and soil analysis in the laboratory andin situ. The17O‐excess flagged the presence of MeOH and EtOH. Adding measurement of δ17O values to traditional measurement of δ2H and δ18O values may shed new light on plant water analysis for source mixing dynamics using DVE.