bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visitors’ experiences of public and private dental care in Sweden in 1992–2012
Authors: Pälvärinne Raimo , Birkhed Dowen , Forsberg Birger , Widström Eeva 
Published: August 2019

Abstract
Aim
The aim was to compare adult patients’ experiences of public and private dental care in Sweden over time from the ages of 50 and 70 years, between 1992 and 2012.

Materials and methods
Data on visiting patterns, oral health, fees and satisfaction were obtained from a questionnaire study every 5 years in 1992–2012 and analysed by using the Chi-square test and logistic regression. In the present study, the answers given by 6083 respondents in 1992 and 5220 in 2012 were included.

Results
Of the 50-year olds, 73.5% had visited the private sector and 26.5% the public sector. In 1992, patients in the public dental service (PDS) had visited their dentists less frequently and experienced having a slightly poorer dental status compared with private patients. After 20 years (2012), the distribution of patients between the two sectors was almost the same (71.4% and 28.8%) and the differences in visiting pattern and dental health persisted. During the study period, 21.6% of the patients changed treatment sector. A small proportion of patients had high treatment costs. A larger proportion of the private sector visitors than the PDS visitors were consistently satisfied with the dental care they had received.

Conclusions
As a whole, most adult patients in Sweden were satisfied with their dental care at both public and private clinics.
Downregulation of eukaryotic translation initiation factor 3b inhibited proliferation and metastasis of gastric cancer
Authors: Ma Fang , Li Xue , Ren Juchao , Guo Ruiting , Li Yuwei , Liu Jichang , Sun Yundong , Liu Zhifang , Jia Jihui , Li Wenjuan 
Published: August 2019

AbstractEukaryotic translation initiation factor 3 (eIF3) plays an important role in the regulation of mRNA translation, cell growth and cancer development. eIF3b is the main scaffolding subunit in the eIF3 complex and has been demonstrated to contribute to the development of several cancers. First, our study found that the downregulation of eIF3b could inhibit the proliferation and metastasis of gastric cancer cells by regulating the expression of cancer-related genes. In addition, the expression of eIF3b correlated with the stage and progression of gastric cancer and was shown to be upregulated in human chronic gastritis and in gastric cancer tissues compared with the expression of eIF3b in normal gastric tissues. Moreover, Helicobacter pylori (H. pylori) infection could upregulate the expression of eIF3b in gastric cancer cells, suggesting that eIF3b might be involved in the carcinogenic process of H. pylori. The above findings identified the oncogenic role of eIF3b in gastric cancer development, and this may contribute to the exploration and discovery of novel therapeutic targets for gastric cancer treatment.
Ferroportin downregulation promotes cell proliferation by modulating the Nrf2–miR-17-5p axis in multiple myeloma
Authors: Kong Yuanyuan , Hu Liangning , Lu Kang , Wang Yingcong , Xie Yongsheng , Gao Lu , Yang Guang , Xie Bingqian , He Wan , Chen Gege , Wu Huiqun , Wu Xiaosong , Zhan Fenghuang , Shi Jumei 
Published: August 2019

AbstractRecent findings demonstrate that aberrant downregulation of the iron-exporter protein, ferroportin (FPN1), is associated with poor prognosis and osteoclast differentiation in multiple myeloma (MM). Here, we show that FPN1 was downregulated in MM and that clustered regularly interspaced short palindromic repeat (CRISPR)-mediated FPN1 knockout promoted MM cell growth and survival. Using a microRNA target-scan algorithm, we identified miR-17-5p as an FPN1 regulator that promoted cell proliferation and cell cycle progression, and inhibited apoptosis—both in vitro and in vivo. miR-17-5p inhibited retarded tumor growth in a MM xenograft model. Moreover, restoring FPN1 expression at least partially abrogated the biological effects of miR-17-5p in MM cells. The cellular iron concentration regulated the expression of the iron-regulatory protein (IRP) via the 5′-untranslated region of IRP messenger RNA and modulated the post-transcriptional stability of FPN1. Bioinformatics analysis with subsequent chromatin immunoprecipitation-polymerase chain reaction and luciferase activity experiments revealed that the transcription factor Nrf2 drove FPN1 transcription through promoter binding and suppressed miR-17-5p (which also increased FPN1 expression). Nrf2-mediated FPN1 downregulation promoted intracellular iron accumulation and reactive oxygen species. Our study links FPN1 transcriptional and post-transcriptional regulation with MM cell growth and survival, and validates the prognostic value of FPN1 and its utility as a novel therapeutic target in MM.
The Eleanor ncRNAs activate the topological domain of the ESR1 locus to balance against apoptosis
Authors: Abdalla Mohamed Osama Ali , Yamamoto Tatsuro , Maehara Kazumitsu , Nogami Jumpei , Ohkawa Yasuyuki , Miura Hisashi , Poonperm Rawin , Hiratani Ichiro , Nakayama Hideki , Nakao Mitsuyoshi , Saitoh Noriko 
Published: August 2019

AbstractMCF7 cells acquire estrogen-independent proliferation after long-term estrogen deprivation (LTED), which recapitulates endocrine therapy resistance. LTED cells can become primed for apoptosis, but the underlying mechanism is largely unknown. We previously reported that Eleanor non-coding RNAs (ncRNAs) upregulate the ESR1 gene in LTED cells. Here, we show that Eleanors delineate the topologically associating domain (TAD) of the ESR1 locus in the active nuclear compartment of LTED cells. The TAD interacts with another transcriptionally active TAD, which is 42.9 Mb away from ESR1 and contains a gene encoding the apoptotic transcription factor FOXO3. Inhibition of a promoter-associated Eleanor suppresses all genes inside the Eleanor TAD and the long-range interaction between the two TADs, but keeps FOXO3 active to facilitate apoptosis in LTED cells. These data indicate a role of ncRNAs in chromatin domain regulation, which may underlie the apoptosis-prone nature of therapy-resistant breast cancer cells and could be good therapeutic targets.
Integrating tough Antheraea pernyi silk and strong carbon fibres for impact-critical structural composites
Authors: Yang Kang , Guan Juan , Numata Keiji , Wu Change , Wu Sujun , Shao Zhengzhong , Ritchie Robert O. 
Published: August 2019

AbstractHigh stiffness and strength carbon fibres are commonly used to reinforce epoxy-resin composites. While wildAntheraea pernyisilk fibres exhibit high toughness originating from their α-helix/random coil conformation structures and their micro-fibre morphology, their insufficient strength and stiffness hinders them from being used in similar structural composites. In this work, we use interply hybridization of silk and carbon fibres to reinforce epoxy-matrix composites. With increased carbon fibre content, the quasi-static tensile/flexural stiffness and strength increases following the rule of mixtures while more silk fibre acts to increase ductility and impact strength. This results in a composite comprising equal volumes of carbon and silk fibres achieving an impact strength of 98 kJ m−2, which is twice that of purely carbon-fibre reinforced composites (44 kJ m−2). This work shows tough natural silk fibres and strong synthetic fibres can be successfully integrated into epoxy-resin composites for tailored mechanical properties.
Evolutionary modification of AGS protein contributes to formation of micromeres in sea urchins
Authors: Poon Jessica , Fries Annaliese , Wessel Gary M. , Yajima Mamiko 
Published: August 2019

AbstractEvolution is proposed to result, in part, from acquisition of new developmental programs. One such example is the appearance of the micromeres in a sea urchin that form by an asymmetric cell division at the 4th embryonic cleavage and function as a major signaling center in the embryo. Micromeres are not present in other echinoderms and thus are considered as a derived feature, yet its acquisition mechanism is unknown. Here, we report that the polarity factor AGS and its associated proteins are responsible for micromere formation. Evolutionary modifications of AGS protein seem to have provided the cortical recruitment and binding of AGS to the vegetal cortex, contributing to formation of micromeres in the sea urchins. Indeed, introduction of sea urchin AGS into the sea star embryo induces asymmetric cell divisions, suggesting that the molecular evolution of AGS protein is key in the transition of echinoderms to micromere formation and the current developmental style of sea urchins not seen in other echinoderms.
Visualization and quantification of inhomogeneous and anisotropic magnetic fields by polarized neutron grating interferometry
Authors: Valsecchi Jacopo , Harti Ralph P. , Raventós Marc , Siegwart Muriel D. , Morgano Manuel , Boillat Pierre , Strobl Markus , Hautle Patrick , Holitzner Lothar , Filges Uwe , Treimer Wolfgang , Piegsa Florian M. , Grünzweig Christian 
Published: August 2019

AbstractThe intrinsic magnetic moment of a neutron, combined with its charge neutrality, is a unique property which allows the investigation of magnetic phenomena in matter. Here we present how the utilization of a cold polarized neutron beam in neutron grating interferometry enables the visualization and characterization of magnetic properties on a microscopic scale in macroscopic samples. The measured signal originates from the phase shift induced by the magnetic potential. Our method enables the detection of previously inaccessible magnetic field gradients, in the order of T cm−1, extending the probed range by an order of magnitude. We visualize and quantify the phase shift induced by a well-defined square shaped uniaxial magnetic field and validate our experimental findings with theoretical calculations based on Hall probe measurements of the magnetic field distribution. This allows us to further extend our studies to investigations of inhomogeneous and anisotropic magnetic field distribution.
Cortical reliability amid noise and chaos
Authors: Nolte Max , Reimann Michael W. , King James G. , Markram Henry , Muller Eilif B. 
Published: August 2019

AbstractTypical responses of cortical neurons to identical sensory stimuli appear highly variable. It has thus been proposed that the cortex primarily uses a rate code. However, other studies have argued for spike-time coding under certain conditions. The potential role of spike-time coding is directly limited by the internally generated variability of cortical circuits, which remains largely unexplored. Here, we quantify this internally generated variability using a biophysical model of rat neocortical microcircuitry with biologically realistic noise sources. We find that stochastic neurotransmitter release is a critical component of internally generated variability, causing rapidly diverging, chaotic recurrent network dynamics. Surprisingly, the same nonlinear recurrent network dynamics can transiently overcome the chaos in response to weak feed-forward thalamocortical inputs, and support reliable spike times with millisecond precision. Our model shows that the noisy and chaotic network dynamics of recurrent cortical microcircuitry are compatible with stimulus-evoked, millisecond spike-time reliability, resolving a long-standing debate.
Climate change exacerbates hurricane flood hazards along US Atlantic and Gulf Coasts in spatially varying patterns
Authors: Marsooli Reza , Lin Ning , Emanuel Kerry , Feng Kairui 
Published: August 2019

AbstractOne of the most destructive natural hazards, tropical cyclone (TC)–induced coastal flooding, will worsen under climate change. Here we conduct climatology–hydrodynamic modeling to quantify the effects of sea level rise (SLR) and TC climatology change (under RCP 8.5) on late 21st century flood hazards at the county level along the US Atlantic and Gulf Coasts. We find that, under the compound effects of SLR and TC climatology change, the historical 100-year flood level would occur annually in New England and mid-Atlantic regions and every 1–30 years in southeast Atlantic and Gulf of Mexico regions in the late 21st century. The relative effect of TC climatology change increases continuously from New England, mid-Atlantic, southeast Atlantic, to the Gulf of Mexico, and the effect of TC climatology change is likely to be larger than the effect of SLR for over 40% of coastal counties in the Gulf of Mexico.
Octahedral gold-silver nanoframes with rich crystalline defects for efficient methanol oxidation manifesting a CO-promoting effect
Authors: Xiong Likun , Sun Zhongti , Zhang Xiang , Zhao Liang , Huang Peng , Chen Xiwen , Jin Huidong , Sun Hao , Lian Yuebin , Deng Zhao , Rümmerli Mark H. , Yin Wanjian , Zhang Duo , Wang Shuao , Peng Yang 
Published: August 2019

AbstractThree-dimensional bimetallic nanoframes with high spatial diffusivity and surface heterogeneity possess remarkable catalytic activities owing to their highly exposed active surfaces and tunable electronic structure. Here we report a general one-pot strategy to prepare ultrathin octahedral Au3Ag nanoframes, with the formation mechanism explicitly elucidated through well-monitored temporal nanostructure evolution. Rich crystalline defects lead to lowered atomic coordination and varied electronic states of the metal atoms as evidenced by extensive structural characterizations. When used for electrocatalytic methanol oxidation, the Au3Ag nanoframes demonstrate superior performance with a high specific activity of 3.38 mA cm−2, 3.9 times that of the commercial Pt/C. More intriguingly, the kinetics of methanol oxidation on the Au3Ag nanoframes is counter-intuitively promoted by carbon monoxide. The enhancement is ascribed to the altered reaction pathway and enhanced OH−co-adsorption on the defect-rich surfaces, which can be well understood from the d-band model and comprehensive density functional theory simulations.
Vegfc/d-dependent regulation of the lymphatic vasculature during cardiac regeneration is influenced by injury context
Authors: Vivien Céline J. , Pichol-Thievend Cathy , Sim Choon Boon , Smith Jacob B. , Bower Neil I. , Hogan Benjamin M. , Hudson James E. , Francois Mathias , Porrello Enzo R. 
Published: August 2019

AbstractThe lymphatic vasculature mediates essential physiological functions including fluid homeostasis, lipid and hormone transport, and immune cell trafficking. Recent studies have suggested that promoting lymphangiogenesis enhances cardiac repair following injury, but it is unknown whether lymphangiogenesis is required for cardiac regeneration. Here, we describe the anatomical distribution, regulation, and function of the cardiac lymphatic network in a highly regenerative zebrafish model system using transgenic reporter lines and loss-of-function approaches. We show that zebrafish lacking functional vegfc and vegfd signaling are devoid of a cardiac lymphatic network and display cardiac hypertrophy in the absence of injury, suggesting a role for these vessels in cardiac tissue homeostasis. Using two different cardiac injury models, we report a robust lymphangiogenic response following cryoinjury, but not following apical resection injury. Although the majority of mutants lacking functional vegfc and vegfd signaling were able to mount a full regenerative response even in the complete absence of a cardiac lymphatic vasculature, cardiac regeneration was severely impaired in a subset of mutants, which was associated with heightened pro-inflammatory cytokine signaling. These findings reveal a context-dependent requirement for the lymphatic vasculature during cardiac growth and regeneration.
Inference-assisted intelligent crystallography based on preliminary data
Authors: Hoshino Manabu , Nakanishi-Ohno Yoshinori , Hashizume Daisuke 
Published: August 2019

AbstractCrystal structure analysis is routinely used to determine atomically resolved molecular structures and structure-property relationships. The accumulation of reliable structural characteristics obtained by crystal structure analysis has forged a robust basis that is frequently used in molecular and materials sciences. However, experimental techniques remain hampered by time-consuming ‘blind’ measurement-analysis iterations, which are sometimes required to find appropriate crystals and experimental conditions. Herein, we present a method that uses a small preliminary data set to evaluate the to-be-observed structures and the to-be-collected data. Moreover, we demonstrate the practical utility of this method to improve the efficiency of crystal structure analysis. This method will help selecting suitable crystals and choosing favorable experimental conditions to generate results that satisfy the level of precision required for specific research objectives.
CLIC4 regulates late endosomal trafficking and matrix degradation activity of MMP14 at focal adhesions in RPE cells
Authors: Hsu Kuo-Shun , Otsu Wataru , Li Yao , Wang Heuy-Ching , Chen Shuibing , Tsang Stephen H. , Chuang Jen-Zen , Sung Ching-Hwa 
Published: August 2019

AbstractDysregulation in the extracellular matrix (ECM) microenvironment surrounding the retinal pigment epithelium (RPE) has been implicated in the etiology of proliferative vitreoretinopathy and age-related macular degeneration. The regulation of ECM remodeling by RPE cells is not well understood. We show that membrane-type matrix metalloproteinase 14 (MMP14) is central to ECM degradation at the focal adhesions in human ARPE19 cells. The matrix degradative activity, but not the assembly, of the focal adhesion is regulated by chloride intracellular channel 4 (CLIC4). CLIC4 is co-localized with MMP14 in the late endosome. CLIC4 regulates the proper sorting of MMP14 into the lumen of the late endosome and its proteolytic activation in lipid rafts. CLIC4 has the newly-identified “late domain” motif that binds to MMP14 and to Tsg101, a component of the endosomal sorting complex required for transport (ESCRT) complex. Unlike the late domain mutant CLIC4, wild-type CLIC4 can rescue the late endosomal sorting defect of MMP14. Finally, CLIC4 knockdown inhibits the apical secretion of MMP2 in polarized human RPE monolayers. These results, taken together, demonstrate that CLIC4 is a novel matrix microenvironment modulator and a novel regulator for late endosomal cargo sorting. Moreover, the late endosomal sorting of MMP14 actively regulates its surface activation in RPE cells.
The Zea mays mutants opaque2 and opaque16 disclose lysine change in waxy maize as revealed by RNA-Seq
Authors: Wang Wei , Niu Suzhen , Dai Yi , Wang Mingchun , Li Yan , Yang Wenpeng , Zhao Degang 
Published: August 2019

AbstractIn maize, opaque2 (o2) and opaque16 (o16) alleles can increase lysine content, while the waxy (wx) gene can enhance the amylopectin content of grains. In our study, o2 and o16 alleles were backcrossed into waxy maize line (wxwx). The o2o2o16o16wxwx lines had amylopectin contents similar to those of waxy line. Their nutritional value was better than waxy line, but the mechanism by which the o2 and o16 alleles increased the lysine content of waxy maize remained unclear. The o2o2o16o16wxwx lines and their parents on kernels (18th day after pollination) were subjected to RNA sequencing (RNA-Seq). The RNA-Seq analysis revealed 272 differentially expressed genes (DEGs). Functional analyses revealed that these DEGs were mainly related to biomass metabolism. Among them, in o2o2o16o16wxwx lines, 15 genes encoding α-zein were down-regulated, which resulted in the reduction of α-zein synthesis and increased lysine content; lkr/sdh1 and Zm00001d020984.1 genes involved in the lysine degradation pathway were down-regulated, thereby inhibited lysine degradation; sh2, bt2 and ae1 genes involved in starch metabolism were upregulated, leaded to wrinkling kernel and farinaceous endosperm. Our transcriptional-level identification of key genes responsible for increased grain lysine content and farinaceous endosperm formation following introgression of o2 and o16 alleles should promote molecular breeding for maize quality.
Systemically Administered Plant Recombinant Holo-Intrinsic Factor Targets the Liver and is not Affected by Endogenous B12 levels
Authors: Workinger Jayme L. , Kuda-Wedagedara Akhila N. W. , Julin Mara M. , White Jordan M. , Nexo Ebba , Viola Nerissa T. , Doyle Robert P. 
Published: August 2019

AbstractPrecision targeting imaging agents and/or treatment agents to select cells or organs in the body remains a significant need and is an area of intense research. It has been hypothesized that the vitamin B12 (B12) dietary pathway, or components thereof, may be exploitable in this area. The question of whether gastric Intrinsic factor (IF), critical for B12 absorption in the GI tract via the cubilin receptor, could be used as a targeting moiety for the cubilin receptor systemically, has not been investigated. Cubilin is the only known receptor for holo-IF and is found primarily in the kidney and ear (outside of the ileum of the GI) offering significant scope for specific targeting. We utilized plant derived human gastric IF in fluorescent cell and PET based in vivo imaging and biodistribution studies and demonstrated that plant derived IF primarily targets the liver, likely a consequence of the unique glycosylation profile of the IF, and is not affected by endogenous B12 levels.
Empathic perspective taking promotes interpersonal coordination through music
Authors: Novembre Giacomo , Mitsopoulos Zoe , Keller Peter E. 
Published: August 2019

AbstractCoordinated behavior promotes collaboration among humans. To shed light upon this relationship, we investigated whether and how interpersonal coordination is promoted by empathic perspective taking (EPT). In a joint music-making task, pairs of participants rotated electronic music-boxes, producing two streams of musical sounds that were meant to be played synchronously. Participants – who were not musically trained – were assigned to high and low EPT groups based on pre-experimental assessments using a standardized personality questionnaire. Results indicated that high EPT pairs were generally more accurate in synchronizing their actions. When instructed to lead the interaction, high and low EPT leaders were equally cooperative with followers, making their performance tempo more regular, presumably in order to increase their predictability and help followers to synchronize. Crucially, however, high EPT followers were better able to use this information to predict leaders’ behavior and thus improve interpersonal synchronization. Thus, empathic perspective taking promotes interpersonal coordination by enhancing accuracy in predicting others’ behavior while leaving the aptitude for cooperation unaltered. We argue that such predictive capacity relies on a sensorimotor mechanism responsible for simulating others’ actions in an anticipatory manner, leading to behavioral advantages that may impact social cognition on a broad scale.