bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial Dysfunction in Primary Ovarian Insufficiency
Authors: Tiosano Dov , Mears Jason A , Buchner David A 
Published: August 2019

Abstract
Primary ovarian insufficiency (POI) is defined by the loss or dysfunction of ovarian follicles associated with amenorrhea before the age of 40. Symptoms include hot flashes, sleep disturbances, and depression, as well as reduced fertility and increased long-term risk of cardiovascular disease. POI occurs in ∼1% to 2% of women, although the etiology of most cases remains unexplained. Approximately 10% to 20% of POI cases are due to mutations in a single gene or a chromosomal abnormality, which has provided considerable molecular insight into the biological underpinnings of POI. Many of the genes for which mutations have been associated with POI, either isolated or syndromic cases, function within mitochondria, including MRPS22, POLG, TWNK, LARS2, HARS2, AARS2, CLPP, and LRPPRC. Collectively, these genes play roles in mitochondrial DNA replication, gene expression, and protein synthesis and degradation. Although mutations in these genes clearly implicate mitochondrial dysfunction in rare cases of POI, data are scant as to whether these genes in particular, and mitochondrial dysfunction in general, contribute to most POI cases that lack a known etiology. Further studies are needed to better elucidate the contribution of mitochondria to POI and determine whether there is a common molecular defect in mitochondrial function that distinguishes mitochondria-related genes that when mutated cause POI vs those that do not. Nonetheless, the clear implication of mitochondrial dysfunction in POI suggests that manipulation of mitochondrial function represents an important therapeutic target for the treatment or prevention of POI.
Intramyocardial Triglycerides Among Women With vs Without HIV: Hormonal Correlates and Functional Consequences
Authors: Toribio Mabel , Neilan Tomas G , Awadalla Magid , Stone Lauren A , Rokicki Adam , Rivard Corinne , Mulligan Connor P , Cagliero Diana , Fourman Lindsay T , Stanley Takara L , Ho Jennifer E , Triant Virginia A , Burdo Tricia H , Nelson Michael D , Szczepaniak Lidia S , Zanni Markella V 
Published: August 2019

Abstract

Context
Women with HIV (WHIV) on anti-retroviral therapy (ART) are living longer but facing heightened vulnerability to heart failure.

Objective
We investigated metabolic/hormonal/immune parameters relating to diastolic dysfunction—a precursor to heart failure—among WHIV without known cardiovascular disease (CVD).

Design and Outcome Measures
Nineteen ART-treated WHIV and 11 non-HIV-infected women without known CVD enrolled and successfully completed relevant study procedures [cardiac magnetic resonance spectroscopy (MRS) and cardiac MRI]. Groups were matched on age and body mass index. Primary outcome measures included intramyocardial triglyceride content (cardiac MRS) and diastolic function (cardiac MRI). Relationships between intramyocardial triglyceride content and clinical parameters were also assessed.

Results
Among WHIV (vs non-HIV-infected women), intramyocardial triglyceride content was threefold higher [1.2 (0.4, 3.1) vs 0.4 (0.1, 0.5)%, P = 0.01], and diastolic function was reduced (left atrial passive ejection fraction: 27.2 ± 9.6 vs 35.9 ± 6.4%, P = 0.007). There was a strong inverse relationship between intramyocardial triglyceride content and diastolic function (ρ = −0.62, P = 0.004). Among the whole group, intramyocardial triglyceride content did not relate to chronologic age but did increase across the reproductive aging spectrum (P = 0.02). HIV status and reproductive aging status remained independent predictors of intramyocardial triglyceride content after adjusting for relevant cardiometabolic parameters (overall model R2 = 0.56, P = 0.003; HIV status P = 0.01, reproductive aging status P = 0.02).

Conclusions
For asymptomatic WHIV, increased intramyocardial triglyceride content is associated with diastolic dysfunction. Moreover, relationships between intramyocardial triglyceride accumulation and women’s reproductive aging are noted.
Pregnancy and Live Birth In Women With Pathogenic LHCGR Variants Using Their Own Oocytes
Authors: Lu Xuefeng , Yan Zheng , Cai Renfei , Khor Shuzin , Wu Ling , Sun Lihua , Wang Yun , Xu Yao , Tian Hui , Chen Qiuju , Qiao Jie , Li Bing , Chen Biaobang , Cao Yu , Lyu Qifeng , Wang Lei , Kuang Yanping 
Published: August 2019

Abstract

Context
The LH/chorionic gonadotropin receptor (LHCGR) is mainly expressed in gonads and plays important roles in estradiol production, ovulation, and luteal formation. Women with pathogenic LHCGR variants suffer from infertility, and successful fertility treatments for such women have never been reported.

Objective
The purpose of this study was to determine whether women with pathogenic LHCGR variants can achieve successful pregnancies through in vitro fertilization.

Design
Three women with LH resistance and infertility and their parents underwent exome sequencing. The biochemical characteristics and functional effects of LHCGR mutation were assessed in transfected human embryonic kidney -293T cells and primary granulosa cells.

Results
All affected women harbored pathogenic LHCGR variants. The LHCGR variants lacked cell surface localization and signal transduction abilities in vitro and in vivo. After dual triggering and prolonging the interval between triggering and oocyte pick-up, all three patients achieved oocytes and high-quality embryos. After frozen embryo transfer, one woman successfully birthed twins, and one woman successfully birthed a live boy. Apart from difficulties in oocyte retrieval, no obvious abnormalities in fertilization or during embryo development and pregnancy were identified in these patients.

Conclusions
This study is, to our knowledge, the first to report successful assisted reproductive treatment of women with pathogenic LHCGR variants using their own oocytes. Our results supported that defects in LHCGR disrupted ovulation but had no effect on fertilization and embryo development.
Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples
Authors: Benhalima Lamia , Amri Sandra , Bensouilah Mourad , Ouzrout Rachid 
Published: August 2019

Background and Objective: With the emergence of antibiotic resistance and the hospital acquired infection, the interest for antimicrobial agents has recently increased again in public health. Copper is recommended as a supplementary method of increasing biological safety in the hospital environment. The objective of this study was to determine the antibacterial activity of copper sulfate salts on strains of bacterial pathogens isolated from different clinical pictures in different health establishment in Algeria.
Methods: A total of 25 different bacterial isolates (16 Enterobacteriaceae, 5 Staphylococci, and 4 Pseudomonas) were tested for susceptibility to copper sulfate using minimum inhibitory concentration (MIC-Cu) and minimum bactericidal concentrations (MBC-Cu) determinations. All isolates were also tested for susceptibility to six antibiotics.
Results: Antibiotic susceptibility studies revealed that 100% of isolates were resistant to one or more antibiotics. Fifty two percent of isolates were very susceptible to copper sulfate, with MICs ranging from 100 to 200 µg/ml. MBC-Cu = 1600 μg/ml showed the best bactericidal effect against the great majority of studied bacteria (52%). A good bactericidal activities of copper sulfate were recorded against Proteus vulgaris and Staphylococcus aureus (MBC/MIC=1). The Gram-negative bacteria isolates which were copper resistant also showed a high resistance to chloramphenicol (r=0.78) and Trimethoprime (r=0.61). Furthermore, the strains that were no-susceptible to three different antimicrobial classes (Escherichia coli, Staphylococcus saprophyticus) were not resistant to copper sulfate.
Conclusion: Copper sulfate salts has significant antibacterial activity against multi-drug resistant nosocomial pathogens.
doi: https://doi.org/10.12669/pjms.35.5.336
How to cite this:Benhalima L, Amri S, Bensouilah M, Ouzrout R. Antibacterial effect of copper sulfate against multi-drug resistant nosocomial pathogens isolated from clinical samples. Pak J Med Sci. 2019;35(5):1322-1328. doi: https://doi.org/10.12669/pjms.35.5.336
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
World Federation for Medical Education: Appropriateness of Basic Medical Education standards in Pakistan
Authors: Wajid Gohar , Sethi Ahsan , Khan Rehan Ahmed , Aamir Hira Shireen 
Published: August 2019

Objective: To explore the appropriateness of WFME Basic Medical Education (BME) standards to Pakistani context.
Methods: A descriptive cross-sectional survey of faculty, graduates and students of five Masters’ in Health Professions Education programmes was carried out from Jul-Dec 2017. Participants were asked to rate the appropriateness of WFME-BME basic standards to Pakistani context on a fourpoint Likert scale (Strongly Disagree through to Strongly Agree). They were also asked for additional comments, if any. Descriptive statistics were carried out for quantitative data, while content analysis for qualitative data.
Results: A total of 127/200 participants responded (63.5%). For all major areas (106 standards), 97.7% responses (n=13,149) were positive while only 2.3% (n=313) were negative. Ninety-six standards got more than 95% positive response while only 10 standards in three major areas got more than five percent negative response. These include five standards from major area Mission and Outcomes, one from Educational Programme and four from Students.
Conclusions: This is the first study exploring the appropriateness of WFME-BME standards for accreditation in Pakistan. We found that all the areas, sub-areas and standards are largely appropriate. We recommend further deliberation on 10 standards with more than five percent negative responses, regarding their contextualization to Pakistan and the development of guidelines and possible reconsiderations in WFME future revisions.
doi: https://doi.org/10.12669/pjms.35.5.882
How to cite this:Wajid G, Sethi A, Khan RA, Aamir HS. World Federation for Medical Education: Appropriateness of Basic Medical Education standards in Pakistan. Pak J Med Sci. 2019;35(5):1185-1191. doi: https://doi.org/10.12669/pjms.35.5.882
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
A novel comparison of Epstein-Barr virus with broad histological spectrum of oral squamous cell carcinoma
Authors: Saleem Muhammad Wasif , Baig Faraz Ahmed , Hadi Naila Irum 
Published: August 2019

Objectives: To evaluate the immunohistochemical expression of Epstein-Barr virus (EBV) in broad spectrum histological subtypes of oral squamous cell carcinoma (OSCC) and to determine the relationship of EBV with clinicopathological parameters of OSCC.
Methods: This cross-sectional study included 150 clinically diagnosed OSCC cases from the outpatient of Ziauddin University Hospital from March, 2017 to October, 2018. These were confirmed on histological examination and categorized into conventional squamous cell carcinoma (SCC) and rare variants. Conventional SCC was subcategorized into keratinizing (KSCC), non-keratinizing (NKSCC), and hybrid SCC (HSCC). EBV status was compared among various histological tumor entities and clinicopathological characteristics of OSCC using immunohistochemistry. Chi-square test was used to determine the association of each histological subtype with EBV status with P-value <0.05 considered as statistically significant.
Results: Conventional tumor was the most frequent squamous cell carcinoma (n=126; 84%). A significant statistical link of EBV infection was observed with rare histological tumors exhibiting acantholysis (P=0.01), as well as tumors involving buccal mucosa (P=0.03), and habitual smokers (P=0.001).
Conclusions: In this study, acantholytic tumor, a rare histological subtype of OSCC, tended to be EBV related. Moreover, OSCC cases bearing EBV infection were more likely smokers favoring buccal mucosa as primary anatomical site for oral cancer.
doi: https://doi.org/10.12669/pjms.35.5.899
How to cite this:Saleem MW, Baig FA, Hadi NI. A novel comparison of Epstein-Barr virus with broad histological spectrum of oral squamous cell carcinoma. Pak J Med Sci. 2019;35(5):1192-1198. doi: https://doi.org/10.12669/pjms.35.5.899
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RETRACTED: Consumer perspectives on reproductive health after using the Jakpros smart mobile health application: A cross-sectional, qualitative study
Authors: Wiweko Budi , Riyanti Aida , Olivia Shanty , Priangga Muhammad , Silvana Vita , Putro Aruan Lewis , Wibawa Yohanes Satrya , Pertiwi Ilonna Putri , Zakirah Sarah Chairani , Harzif Achmad Kemal , Pratama Gita , Muharam R. , Hestiantoro Andon 
Published: August 2019

The article titled “Consumer perspectives on reproductive health after using the Jakpros smart mobile health application: A cross-sectional, qualitative study” ([version 1; peer review: 1 not approved] F1000Research 2019, 8: 396 https://doi.org/10.12688/f1000research.17894.1) has been retracted by F1000Research on the grounds of scientific misconduct. On submission to F1000Research, all manuscripts undergo plagiarism checks as standard—this is detailed in our Originality policies. Upon submission, authors are also required to declare that the article is not under consideration or review by another publisher. At this stage in the publication process, our plagiarism detection software did not identify any significant duplication of text. Following publication of the article, the F1000Research editorial team were notified of a high degree of similarity between this manuscript and one already published in the Institute of Physics (IOP) Conference Series: Earth and Environmental Science (B Wiweko et al 2019 IOP Conf. Ser.: Earth Environ. Sci. 248 012038 https://iopscience.iop.org/article/10.1088/1755-1315/248/1/012038/pdf). On investigation, the extent of overlap between these two publications was substantial, and this was deemed a case of duplicate publication. Duplicate publication is a major ethical issue and, as a member of the Committee on Publication Ethics (COPE) and a follower of the International Committee of Medical Journal Editors (ICMJE) recommendations, F1000Research takes such matters seriously. In response to our concerns, corresponding author Budi Wiweko acknowledged that publication misconduct has taken place and has been informed that, due to the extent and nature of duplication between the articles published in F1000Research and IOP, F1000Research will retract the article and publish a linked retraction notice. In line with COPE guidelines, F1000Research has informed the Publisher of IOP Conference Series about this issue, and our final decision has been communicated to all authors and their respective institutions.
SWOT Analysis of Eco-Labels and their Projection on Sustainability Spectrum: A Theoretical Evaluation of Destination India
Authors: Akhter Shamima , Darzi M A 
Published: August 2019

Most of the organisations in India follow contemporary practices of sustainable development but very few have obtained 'green' certifications, and tourism industry is no exception. A booming economy, with a population of over 1.2 billion, India's infrastructure constructions are soaring, resulting in a depletion of rare flora and fauna species and an increase in pollution of water bodies. Against the backdrop of these three constituents, this paper presents a SWOT analysis of three important ecolabels which covers almost all sectors of the tourism industry. Further, an evaluation of this ecolabel implementation in India is done through sustainability spectrum vis-a-vis sustainability positions.
The Implications of Demonetization and Currency Constraints on Indian Tourism – A Perspective Analysis
Authors: S Mary Jeniffer , Chandran Anu , Gantait Arnab 
Published: August 2019

A landmark decision by the Government of India on the 8th of November 2016, viz, demonetization, propped up monetary repercussions of high magnitude the nation has ever witnessed. The ‘Ifs and Buts’ of demonetization are being widely debated (of course at this juncture the government machinery and banks need to announce the positive outcomes such as stoppage of terrorist funding and black money, etc. as claimed earlier) albeit in the days that followed after the announcement it became explicit that the decision was taken without adequate preparedness. The whole process, though seemingly transparent, has had on impact on the lives of all the citizens leading to the initial optimism expressed by a great number of people waning into contempt and
A Study on the Role and Contribution of Women for the Development of Ayurvedic Education in Kerala
Authors: Thomas Anila 
Published: August 2019

Education has been of central significance to the development of human society. It can be the beginning not only of individual knowledge, information and awareness, but also of a holistic strategy for development and change. Education helps an individual to develop his or her potential to the fullest, to increase the productivity and to become useful and resourceful members of the society. Education is holistic in concept and is multidimensional. Due to the technical advancement and social development, the structure of the family organization is changing. These substantial changes have created a new challenging role and status for the Indian women, especially for the middle class women. The structure of the Indian society is undergoing a metamorphosis due to Urbanisation, Westernisation, Industrialisation and Politicalisation. As a member of the family, she has to perform certain traditional roles, apart from taking an active part in economic activity. The provision of educational opportunities for girls and women has been part of the national endeavour since independence. The constitution of India, is clearly committed to the cause of education and unequivocally endorses state intervention to redress an adverse educational scenario. The recent social
Karnataka Tourism Satellite Accounts (Kar-TSA): A Necessity for the Tourism Industry
Authors: Kumar Mahesh 
Published: August 2019

Tourism is being highlighted for its immense business opportunities with its close linkages to the transport and accommodation industry and a host of other sectors. The potential and performance of the tourism industry in the state of Karnataka, needs to be gauged in terms of its socio-economic magnitudes. The present study focuses on the need for developing a Tourism Satellite Account (TSA) framework, which enables estimating or arriving at tourism activity impact on the state economy and its people, because tourism does not exist as a distinct sector, but happens across several sectors. The TSA framework separates out all tourism-related activity within gross state products (GSP). World Tourism Organization developed this framework and so far, over 75 counties have either initiated or completed implementation of this framework. In 2006, India has published the first National Tourism Satellite Accounts. Efforts are being made to understand the need for adapting the sub-national level TSA for the state of Karnataka with discussions on the TSA conceptual framework and the present situation around the globe as well as in the sub-continent, followed by the findings and recommendations.
Sustainability Issues in River Tourism: A Case Study of Ganges in Varanasi
Authors: Tandon Neha 
Published: August 2019

Understanding future expectations and fulfilling current demands with a view of conserving social, cultural and natural environment of a destination, can be referred to as sustainable tourism. Changes in social and cultural environment are identified a little early than their natural counterpart. The first two being more vocal and narrates quick reactions among its audiences. Changes in natural environment lie silent till the time they reach a visible level of depletion. The present paper will highlight sustainability issues in river tourism, which is one of the significant natural resources for tourism. Understanding the dependency of tourism on rivers and highlighting its positive and negative effects of it, will be discussed in the paper. The paper is supported by a case study on „The Ganges in Varanasi‟. The Holy Ganges is the lifeline of the city and is facing immense sustainability issues, which has raised questions against her future existence. The problems faced and measures taken by various organizations, government bodies, and individuals to protect the lifeline of the oldest living city of the world will be examined further.
Influence of New Media on Travel Decision Making
Authors: Dileep M R , S Sindhu , Ismail Abduraoof Ahmed 
Published: August 2019

Tourism, one of the largest industries in the world, has recorded consistent growth since the end of Second World War. Travel is a key factor in the process of tourism, where the tourist is the main player as well as the ultimate consumer of the products and services offered to him/her. A tourist‟s decision to travel is by itself a complex process because there are many factors that influence the decision. The New media, which has emerged in the wake of tremendous advancements in information and communication technologies is also influencing the decision making process in modern times. Here, an attempt has been done to examine the influence of new media in the various stages of decision making process and the role played by it. A questionnaire based survey was undertaken to conduct the study and the analysis reveals various kinds of influences of new media.
A Web-based GIS for Tourism Administration in Kerala
Authors: C K Venugopal , Ajayakumar V 
Published: August 2019

Geographic Information systems are used widely for Tourism administration in several countries, but not especially true in Kerala scenario. This paper attempts to present a GIS model for tourism administration in Kerala. Various applications of Tourism based GIS in the international arena have been examined in this paper. Data collected from different sources reveals the present status of Tourism GISs world over. The study focuses on areas where GIS can be applied in Kerala Tourism and gives outputs that can be generated using the proposed model. Tourism is essentially a cartographic phenomenon. In this context this paper assumes importance as various devices, both wired and wireless are increasingly used to accesses cartographic information. With internet becoming an unavoidable component of modern day life, map based information is the need of the hour. A web based GIS will benefit Kerala tourism in the coming years. The model proposed in this paper can act as a foundation for building a web based GIS for Kerala tourism.