bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inactivation of the uptake hydrogenase in the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS enables a biological water–gas shift platform for H2 production
Authors: Eckert Carrie A , Freed Emily , Wawrousek Karen , Smolinski Sharon , Yu Jianping , Maness Pin-Ching 
Published: April 2019

Abstract
Biological H2 production has potential to address energy security and environmental concerns if produced from renewable or waste sources. The purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS produces H2 while oxidizing CO, a component of synthesis gas (Syngas). CO-linked H2 production is facilitated by an energy-converting hydrogenase (Ech), while a subsequent H2 oxidation reaction is catalyzed by a membrane-bound hydrogenase (MBH). Both hydrogenases contain [NiFe] active sites requiring 6 maturation factors (HypA-F) for assembly, but it is unclear which of the two annotated sets of hyp genes are required for each in R. gelatinosus CBS. Herein, we report correlated expression of hyp1 genes with Ech genes and hyp2 expression with MBH genes. Moreover, we find that while Ech H2 evolving activity is only delayed when hyp1 is deleted, hyp2 deletion completely disrupts MBH H2 uptake, providing a platform for a biologically driven water–gas shift reaction to produce H2 from CO.
Reputation as a Disciplinarian of International Organizations
Authors: Daugirdas Kristina 
Published: April 2019

AbstractAs a disciplinarian of international organizations, reputation has serious shortcomings. Even though international organizations have strong incentives to maintain a good reputation, reputational concerns will sometimes fail to spur preventive or corrective action. Organizations have multiple audiences, so efforts to preserve a “good” reputation may pull organizations in many different directions, and steps taken to preserve a good reputation will not always be salutary. Recent incidents of sexual violence by UN peacekeepers in the Central African Republic illustrate these points.
Cost of lipid lowering in patients with coronary artery disease by Case Method Learning
Authors: Kiessling Anna , Zethraeus Niklas , Henriksson Peter 
Published: April 2019

Objectives:This investigation was undertaken to study the costs of a Case Method Learning (CML) -supported lipid-lowering strategy in secondary prevention of coronary artery disease (CAD) in primary care.Methods:This prospective randomized controlled trial in primary care with an additional external specialist control group in Södertälje, Stockholm County, Sweden, included 255 consecutive patients with CAD. Guidelines were mailed to all general practitioners (GPs; n=54) and presented at a common lecture. GPs who were randomized to the intervention group participated in recurrent CML dialogues at their primary health-care centers during a 2-year period. A locally well-known cardiologist served as a facilitator. Assessment of low-density lipoprotein (LDL) cholesterol was performed at baseline and after 2 years. Analysis according to intention-to-treat—intervention and control groups (n=88)—was based on group affiliation at baseline. The marginal cost of lipid lowering comprised increased cost of lipid-lowering drugs in the intervention group compared with the primary care control group, cost of attendance of the GP's in the intervention group, and cost of time for preparation, travel, and seminars of the facilitator. Costs are as of 2002 with an exchange rate 1 US$=9.5 SEK (Swedish Crowns).Results:Patients in the primary care intervention group had their LDL cholesterol reduced by 0.5 (confidence interval [CI], 0.1–0.9) mmol/L compared with the primary care control group (p<.05). No change occurred in controls. LDL cholesterol in the external specialist control group decreased by 0.6 (CI, 0.4–0.8) mmol/L. The cost of the educational intervention represented only 2 percent of the drug cost. The cost of lipid lowering in the intervention group, including the cost of the educational intervention, was actually lower than that of patients treated at the specialist clinic—106 US$ per mmol decrease in LDL cholesterol in the intervention group and 153 US$ per mmol decrease in LDL cholesterol in the specialist group. EuroQol 5D Index, which gives an estimate of global health-related quality of life, was 0.80 (CI, 0.75–0.85) in the present cohort.Conclusions:The additional cost of CML was only 2 percent of the drug cost. Assuming the same gain in life expectancy per millimole decrease in LDL cholesterol as in the 4S-study gives a cost per gained quality-adjusted life year of US$ 24,000. This finding indicates that the CML-supported lipid-lowering strategy is cost-effective. The low cost of CML in primary care should probably warrant its use in the improvement of the quality of care in other major chronic diseases.
Data validation in an economic evaluation of surgery for colon cancer
Authors: Janson Martin , Carlsson Per , Haglind Eva , Anderberg Bo 
Published: April 2019

Objectives:This study aimed to validate the accuracy of data retrieved in a prospective multicenter trial, the purpose of which was an economic evaluation of two techniques of surgery for colon cancer.Methods:Within the Swedish contribution of the COLOR trial (Colon Cancer Open or Laparoscopic Resection), an economic evaluation of open versus laparoscopic surgical techniques was conducted. Data were collected by case record forms (CRF), patient diaries, and telephone surveys every 2 weeks. The study period was 12 weeks, and the perspective was societal. Data from the first consecutive forty patients to complete the health economic study protocol were validated. Retrieved data were compared with data from medical records and data from local social security offices for agreement.Results:Statistically significant differences were found for duration of anesthesia, length of surgery, number of outpatient consultations by doctors and district nurses, complication rate, and the use of central venous lines. No significant differences were observed concerning length of hospital stay, disposable instruments cost, and time off work, all of which heavily influence total costs.Conclusions:The present method of data collection regarding resources used in this setting seems to produce accurate data for economic evaluation; however, relative to complication rates, the method did not retrieve accurate data.
CO2 Inflow and Elements Desorption Prior to a Seismic Sequence, Amatrice‐Norcia 2016, Italy
Authors: Boschetti T. , Barbieri M. , Barberio M. D. , Billi A. , Franchini S. , Petitta M. 
Published: April 2019

AbstractThe 2016 Mw ≥ 6.0 Amatrice‐Norcia earthquakes (central Apennines, Italy) and the related seismic sequence were associated with increases in arsenic and vanadium concentrations recorded in groundwater springs a few months before the earthquakes occurred. To evaluate these signals as reliable seismic precursors and effective predictive tools, we studied the geochemical processes that caused these anomalies. Using chemical and isotope models, we show that increased concentrations of arsenic and vanadium, a slight increase in boron concentrations, and a concomitant lowering of the boron isotope ratio may be due to mineral desorption (e.g., from iron oxides and/or clays). We argue that a displacing effect on the trace elements sorbed on minerals was triggered by an excess of deep CO2 in groundwater, which occurred prior to the main seismic event as a result of preseismic crustal dilation. Our observations confirm the pivotal role of CO2 in the release of trace elements by alteration of solid phases and provide a new understanding of earthquake‐related water chemical anomalies.
The fishery performance indicators for global tuna fisheries
Authors: McCluney Jessica K. , Anderson Christopher M. , Anderson James L. 
Published: April 2019

AbstractWe characterize the ecological, economic, and community performance of 21 major tuna fisheries, accounting for at least 77% of global tuna production, using the Fishery Performance Indicators. Our analysis reveals that the biggest variations in performance among tuna fisheries are driven by the final markets that they target: international sashimi market tuna fisheries considerably outperform a comparison set of 62 non-tuna fisheries in the Fishery Performance Indicator database, international canned tuna market fisheries perform similarly to the comparison set, and tuna fisheries supplying local markets in coastal states considerably underperform the comparison set. Differences among regional fishery management organizations primarily reflect regional species composition and market access, despite stark variation in governance, management, and other enabling conditions. With a legacy of open access, tuna’s harvest sector performance is similar across all fisheries, reflecting only a normal return on the capital and skill invested: industrial vessels slightly outperform semi-industrial and artisanal vessels. Differences emerge in the post-harvest sector however, as value chains able to preserve quality and transport fish to high value markets outperform others.
KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Authors: Song Lin , Tang Shi , Han Xiaolei , Jiang Ziying , Dong Lingling , Liu Cuicui , Liang Xiaoyan , Dong Jixin , Qiu Chengxuan , Wang Yongxiang , Du Yifeng 
Published: April 2019

AbstractExosomes are nanosized membrane vesicles released from cells after fusion of multivesicular bodies (MVBs) with the plasma membrane (PM) and play important roles in intercellular communication and numerous biological processes. However, the molecular mechanisms regulating exosome secretion remain poorly understood. Here we identify KIBRA as an adaptor-like protein that stabilizes Rab27a, which in turn controls exosome secretion both in vitro and in vivo. Knockdown or overexpression of KIBRA in neuronal and podocyte cell lines leads to a decrease or increase of exosome secretion, respectively, and KIBRA depletion increases MVB size and number. Comparing protein profiles between KIBRA knockout and wild-type mouse brain showed significantly decreased Rab27a, a small GTPase that regulates MVB-PM docking. Rab27a is stabilized by interacting with KIBRA, which prevents ubiquitination and degradation via the ubiquitin-proteasome pathway. In conclusion, we show that KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a.
Nutrient, pigment, suspended matter and turbidity measurements in the Belgian part of the North Sea
Authors: Mortelmans Jonas , Deneudt Klaas , Cattrijsse André , Beauchard Olivier , Daveloose Ilse , Vyverman Wim , Vanaverbeke Jan , Timmermans Klaas , Peene Jan , Roose Patrick , Knockaert Mark , Chou Lei , Sanders Richard , Stinchcombe Marc , Kimpe Philippe , Lammens Saskia , Theetaert Hannelore , Gkritzalis Thanos , Hernandez Francisco , Mees Jan 
Published: April 2019

AbstractThrough regular sampling surveys, the Flanders Marine Institute is generating long term data series for the Belgian coastal water and sand bank systems, a designated site in the Long Term Ecological Research (LTER) network. The data series is built on sampling activities initiated in 2002, but gradually upgraded and extended in the framework of the LifeWatch marine observatory and the Integrated Carbon Observation System (ICOS) participation. Nine nearshore stations are sampled monthly, with additional seasonal sampling of eight offshore stations. This paper presents the generated data series for nutrients, pigments, suspended matter and turbidity. The collection, methodology and processing of the 2002–2018 dataset is described, along with its data curation, integration and quality control. Yearly versions of the data are published online in a standardized format, accompanied with extensive metadata description and labelled with digital identifiers for traceability. Data is published under a CC-BY license, allowing use of the data under the condition of providing reference to the original source.
Broadband MIR harvester using silicon nanostructures
Authors: Magdi Sara , El-Diwany Farah , A. Swillam Mohamed 
Published: April 2019

AbstractIn this work, we propose an all-silicon-based super absorber in the mid infrared (MIR) spectral range. The presented structures are composed of n-doped silicon nanoparticles or nanowires embedded in intrinsic silicon. An intense absorption peak is observed and could be tuned across the MIR range. While nanoparticles give a single broad absorption peak, the nanowires structure shows a broadband absorption of more than 70% from λ = 5 to 13 µm reaching up to 99% at 7 µm. The absorption peak could be extended to more than 20 µm by increasing the length of the nanowire. Increasing the diameter of the nanoparticles gives higher absorption, reaching just above 90% efficiency at λ = 11 µm for a diameter of 1500 nm. Changing the geometrical parameters of each structure is thoroughly studied and analyzed to obtain highest absorption in MIR. The proposed structures are CMOS compatible, have small footprints and could be integrated for on-chip applications.
Boreal marine fauna from the Barents Sea disperse to Arctic Northeast Greenland
Authors: Andrews Adam J. , Christiansen Jørgen S. , Bhat Shripathi , Lynghammar Arve , Westgaard Jon-Ivar , Pampoulie Christophe , Præbel Kim 
Published: April 2019

AbstractAs a result of ocean warming, the species composition of the Arctic seas has begun to shift in a boreal direction. One ecosystem prone to fauna shifts is the Northeast Greenland shelf. The dispersal route taken by boreal fauna to this area is, however, not known. This knowledge is essential to predict to what extent boreal biota will colonise Arctic habitats. Using population genetics, we show that Atlantic cod (Gadus morhua), beaked redfish (Sebastes mentella), and deep-sea shrimp (Pandalus borealis) recently found on the Northeast Greenland shelf originate from the Barents Sea, and suggest that pelagic offspring were dispersed via advection across the Fram Strait. Our results indicate that boreal invasions of Arctic habitats can be driven by advection, and that the fauna of the Barents Sea can project into adjacent habitats with the potential to colonise putatively isolated Arctic ecosystems such as Northeast Greenland.
Rapid detection of Bacillus ionophore cereulide in food products
Authors: Ducrest P. J. , Pfammatter S. , Stephan D. , Vogel G. , Thibault P. , Schnyder B. 
Published: April 2019

AbstractCereulide is a toxic cyclic depsipeptide produced by certain strains of Bacillus cereus found in soil and food products. While some harmless strains of Bacillus are used as probiotic, others can cause nausea and vomiting, and represent an important food safety concern. Current detection methods are time consuming and do not necessarily detect toxic cereulide. Here, we developed a rapid protocol using Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry that detects the toxin originating from a colony smear of B. cereus. The distinct molecular feature of the toxin peak at m/z 1,191 was clearly identified from bacterial extracts with a limit of detection (LOD) of 30 ng/mL. Final optimisation of the sample preparation was based on cereulide chelating cations to produce the alkali adduct [M + K]+ without the use of a MALDI matrix, and provided a 1,000-fold improvement of LOD with 30 pg/mL of cereulide. We evaluated the application of this method for the detection of cereulide in rice, milk, and different ready-to-eat meals. The proposed protocol is quick, easy and provides an improvement over conventional methods for the detection of B. cereus toxin.
Influence of pH Modification on Catalytic Activities of Metal-Doped IrO2 Nanoparticles
Authors: Kim Joo Yeon , Lee Hangil 
Published: April 2019

AbstractThe effects of pH variation on the catalytic activity of IrO2 nanoparticles (NPs) doped with Cr (an early transition metal) or Ni (a late transition metal) depending on the amount of defect structures on the NP surfaces were analyzed. It was found that both Cr@IrO2 and Ni@IrO2 NPs, fabricated under basic conditions (pH = 13.5) denoted as Cr@IrO2-B and Ni@IrO2-B, respectively, were the best catalysts among the eight tested ones. Moreover, it was confirmed that variation in pH resulted in the changes in the surface area (defect structure), which were considered to be responsible for the changes in the catalytic properties of these NPs. For the oxygen evolution reaction, these NPs exhibited relatively smaller overpotential (η) values than other tested Cr@IrO2- and Ni@IrO2-containing NPs. Furthermore, methylene blue degradation analysis and OH radical formation experiments by benzoic acid showed the same trend. Thus, we confirmed that the catalytic activity of transition metals doped IrO2 NPs fabricated under basic conditions can be improved.
SNP rs2596542G&gt;A in MICA is associated with risk of hepatocellular carcinoma: a meta-analysis
Authors: Wang Haichuan , Cao Hui , Xu Zhong , Wang Dong , Zeng Yong 
Published: April 2019

Abstract
The association of major histocompatibility complex class I chain-related gene A (MICA) single nucleotide polymorphism (SNP) rs2596542G&gt;A and hepatocellular carcinoma (HCC) has been broadly studied, with inconsistent results. Therefore, we conducted the current meta-analysis to better elucidate the roles of SNP rs2596542G&gt;A in HCC. Eligible articles were searched in PubMed, CNKI, Wanfang, Embase, VIP, Web of Science, and CBM databases up to November 2018. Odds ratios (ORs) and 95% CIs were applied. A total of 11 articles, including 4528 HCC patients and 16,625 control subjects, were analyzed. Results revealed that rs2596542G&gt;A was significantly associated with HCC in the heterozygote (G/A versus A/A, P=0.006, OR = 0.854; 95% CI: 0.763–0.956); and dominant (G/G + G/A versus A/A; P=0.021; OR = 0.796; 95% CI: 0.655–0.967) genetic models. Nevertheless, we also detected significant associations between rs2596542G&gt;A and HCV-induced HCC. Additionally, according to our analyses, SNP rs2596542G&gt;A was not correlated with HBV-induced HCC. In conclusion, our findings suggest that MICA SNP rs2596542G&gt;A is associated with HCC susceptibility amongst the Asian, Caucasian, and African ethnicity in certain genetic models. Specifically, MICA SNP rs2396542G&gt;A is associated with risk of HCV-induced HCC, not HBV-induced HCC.
The resistant effect of SIRT1 in oxidative stress-induced senescence of rat nucleus pulposus cell is regulated by Akt-FoxO1 pathway
Authors: He Junsheng , Zhang Ailiang , Song Zhiwen , Guo Shiwu , Chen Yuwei , Liu Zhiyuan , Zhang Jinlong , Xu Xu , Liu Jinbo , Chu Lei 
Published: April 2019

Abstract
Objective: The senescence of nucleus pulposus (NP) cells induced by oxidative stress is one of the important causes of intervertebral disc degeneration (IDD). Herein, we investigated the role and action mechanism of silent information regulator 1 (SIRT1) in oxidative stress-induced senescence of rat NP cell.
Methods: Premature senescence of rat NP cells was induced by sublethal concentration of hydrogen peroxide (H2O2) (100 μM). SIRT1 was activated with SRT1720 (5 μM) to explore its effect on NP cells senescence. FoxO1 and Akt were inhibited by AS1842856 (0.2 μM) and MK-2206 (5 μM), respectively, to explore the role of Akt-FoxO1-SIRT1 axis in rat NP cells. Pretreatment with the resveratrol (20 μM), a common antioxidant and indirect activator of SIRT1, was done to investigate its role in senescent rat NP cells.
Results: The mRNA and protein levels of SIRT1 were decreased in H2O2-induced senescent rat NP cells, and that specific activation of SIRT1 suppresses senescence. And the Akt-FoxO1 pathway, as the upstream of SIRT1, might be involved in the regulation of H2O2-induced senescence of rat NP cells by affecting the expression of SIRT1. In addition, the resveratrol played an anti-senescence role in rat NP cells, which might affect the Akt-FoxO1-SIRT1 axis.
Conclusion: SIRT1 ameliorated oxidative stress-induced senescence of rat NP cell which was regulated by Akt-FoxO1 pathway, and resveratrol exerted anti-senescence effects by affecting this signaling axis.
Myosin Va and spermine synthase: partners in exosome transport
Authors: Timson David J. 
Published: April 2019

Abstract
A recent paper in Bioscience Reports (BSR20182189) describes the discovery of an interaction between the motor protein myosin Va and the metabolic enzyme spermine synthase. Myosin Va is a molecular motor which plays a key role in vesicle transport. Mutations in the gene which encodes this protein are associated with Griscelli syndrome type 1 and the ‘dilute’ phenotype in animals. Spermine synthase catalyzes the conversion of spermidine to spermine. This largely cytoplasmic enzyme can also be localized to the soluble fraction in exosomes. Mutations in the spermine synthase gene are associated with Snyder Robinson mental retardation syndrome. The interaction between the two proteins was detected using the yeast two hybrid method and verified by microscale thermophoresis of recombinant proteins. Knockdown of the MYO5A gene reduced the expression of mRNA coding for spermine synthase. The amount of this transcript was also reduced in cells derived from a patient with Griscelli syndrome type 1. This suggests that, in addition to a direct physical interaction between the two proteins, myosin Va also modulates the transcription of the spermine synthase gene. The mechanism for this modulation is currently unknown. These findings have implications for Griscelli syndrome type 1 and Snyder Robinson mental retardation syndrome. They also suggest that interactions between myosin Va and soluble exosome proteins such as spermine synthase may be important in the mechanism of exosome transport.
Being in a loop: how long non-coding RNAs organise genome architecture
Authors: Pisignano Giuseppina , Pavlaki Ioanna , Murrell Adele 
Published: April 2019

Abstract
Chromatin architecture has a significant impact on gene expression. Evidence in the last two decades support RNA as an important component of chromatin structure [Genes Dev. (2005) 19, 1635–1655; PLoS ONE (2007) 2, e1182; Nat. Genet. (2002) 30, 329–334]. Long non-coding RNAs (lncRNAs) are able to control chromatin structure through nucleosome positioning, interaction with chromatin re-modellers and chromosome looping. These functions are carried out in cis at the site of lncRNAs transcription or in trans at distant loci. While the evidence for a role in lncRNAs in regulating gene expression through chromatin interactions is increasing, there is still very little conclusive evidence for a potential role in looping organisation. Here, we review models for the involvement of lncRNAs in genome architecture and the experimental evidence to support them.