bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of circulating tumor cells in blood by shell-isolated nanoparticle – enhanced Raman spectroscopy (SHINERS) in microfluidic device
Authors: Niciński K. , Krajczewski J. , Kudelski A. , Witkowska E. , Trzcińska-Danielewicz J. , Girstun A. , Kamińska A. 
Published: June 2019

AbstractIsolation and detection of circulating tumor cells (CTCs) from human blood plays an important role in non- invasive screening of cancer evolution and in predictive therapeutic treatment. Here, we present the novel tool utilizing: (i) the microfluidic device with (ii) incorporated photovoltaic (PV) based SERS-active platform, and (iii) shell-isolated nanoparticles (SHINs) for simultaneous separation and label-free analysis of circulating tumour cells CTCs in the blood specimens with high specificity and sensitivity. The proposed microfluidic chip enables the efficient size – based inertial separation of circulating cancer cells from the whole blood samples. The SERS-active platform incorporated into the microfluidic device permits the label-free detection and identification of isolated cells through the insight into their molecular and biochemical structure. Additionally, the silver nanoparticles coated with an ultrathin shell of silica (Ag@SiO2) was used to improve the detection accuracy and sensitivity of analysed tumor cells via taking advantages of shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS). The empirical analysis of SHINERS spectra revealed that there are some differences among studied (HeLa), renal cell carcinoma (Caki-1), and blood cells. Unique SHINERS features and differences in bands intensities between healthy and cancer cells might be associated with the variations in the quantity and quality of molecules such as lipid, protein, and DNA or their structure during the metastasis cancer formation. To demonstrate the statistical efficiency of the developed method and improve the differentiation for circulating tumors cells detection the principal component analysis (PCA) has been performed for all SHINERS data. PCA method has been applied to recognize the most significant differences in SHINERS data among the three analyzed cells: Caki-1, HeLa, and blood cells. The proposed approach challenges the current multi-steps CTCs detection methods in the terms of simplicity, sensitivity, invasiveness, destructivity, time and cost of analysis, and also prevents the defragmentation/damage of tumor cells and thus leads to improving the accuracy of analysis. The results of this research work show the potential of developed SERS based tool for the separation of tumor cells from whole blood samples in a simple and minimally invasive manner, their detection and molecular characterization using one single technology.
Power Doppler ultrasound and contrast-enhanced ultrasound demonstrate non-invasive tumour vascular response to anti-vascular therapy in canine cancer patients
Authors: Abma Eline , Stock Emmelie , De Spiegelaere Ward , Van Brantegem Leen , Vanderperren Katrien , Ni Yicheng , Vynck Matthijs , Daminet Sylvie , De Clercq Kaat , de Rooster Hilde 
Published: June 2019

AbstractCombretastatin A4-phosphate (CA4P) is an anti-vascular agent which selectively shuts down blood supply in tumours, resulting in extensive tumour necrosis. The aim of this study was to assess in vivo, non-invasive ultrasound techniques for the early evaluation of tumour perfusion following CA4P treatment of spontaneous tumours. Eight dogs that bore spontaneous tumours were enrolled and were subsequently treated with a single dose of intravenous CA4P. Perfusion of tumours was evaluated by power Doppler ultrasound (PDUS) pre-treatment (0 h), during the injection (10 min, 20 min, 30 min) and after CA4P infusion (24 and 72 h). Vascularity index (VI) of the tumour tissue was quantitatively analysed and accuracy was verified by correlation analysis with the results of immunohistochemical evaluation of microvessel density (MVD). Central and peripheral perfusion was evaluated by contrast-enhanced ultrasound (CEUS) pre-treatment and at 72 h post-treatment. Post-treatment, PDUS demonstrated a significant decrease in VI within 10 min of CA4P infusion. CEUS parameters demonstrated a significant decrease in blood velocity and volume in the central aspect of the tumour. Histology revealed a 4.4-fold reduction (p < 0.001, 95% CI [2.2,9.4]) in MVD and a 4.1-fold increase (p = 0.003, 95% CI [1.4,11.8]) in necrotic tumour tissue. A strong correlation between PDUS results and immunohistochemical results was found (Pearson R2 = 0.957, p < 0.001). Furthermore, the findings of PDUS were supported by the objective results of the CEUS analyses. These data suggest a role for ultrasound in real-time, non-invasive monitoring of tumour vascular response as an early indicator of CA4P treatment efficacy.
Relevant Word Order Vectorization for Improved Natural Language Processing in Electronic Health Records
Authors: Thompson Jeffrey , Hu Jinxiang , Mudaranthakam Dinesh Pal , Streeter David , Neums Lisa , Park Michele , Koestler Devin C. , Gajewski Byron , Jensen Roy , Mayo Matthew S. 
Published: June 2019

AbstractElectronic health records (EHR) represent a rich resource for conducting observational studies, supporting clinical trials, and more. However, much of the data contains unstructured text, presenting an obstacle to automated extraction. Natural language processing (NLP) can structure and learn from text, but NLP algorithms were not designed for the unique characteristics of EHR. Here, we propose Relevant Word Order Vectorization (RWOV) to aid with structuring. RWOV is based on finding the positional relationship between the most relevant words to predicting the class of a text. This facilitates machine learning algorithms to use the interaction of not just keywords but positional dependencies (e.g. a relevant word occurs 5 relevant words before some term of interest). As a proof-of-concept, we attempted to classify the hormone receptor status of breast cancer patients treated at the University of Kansas Medical Center, comparing RWOV to other methods using the F1 score and AUC. RWOV performed as well as, or better than other methods in all but one case. For F1 score, RWOV had a clear edge on most tasks. AUC tended to be closer, but for HER2, RWOV was significantly better for most comparisons. These results suggest RWOV should be further developed for EHR-related NLP.
Elasmobranch bycatch in the demersal prawn trawl fishery in the Gulf of Papua, Papua New Guinea
Authors: White W. T. , Baje L. , Simpfendorfer C. A. , Appleyard S. A. , Chin A. , Sabub B. , Rochel E. , Naylor G. J. P. 
Published: June 2019

AbstractThe elasmobranch bycatch of the Gulf of Papua Prawn Fishery is investigated in detail for the first time. Fisheries observers collected data on the elasmobranch bycatch from a total of 403 trawl sets (1,273 hrs) in the Gulf of Papua. A total of 40 species of elasmobranchs were recorded ranging in size from a 12 cm disc width stingray to a 350 cm total length sawfish. High mortality rates were recorded (>80%), attributed to the long trawl durations (up to 4 hours). The future inclusion of bycatch reduction devices would likely reduce the number of larger elasmobranchs being caught, based on evidence from the prawn trawl fisheries of northern Australia, and is being investigated by the PNG National Fisheries Authority. Differences in catch compositions were detected across the management zones as well as between the two monsoonal seasons (SE Monsoon and NW Monsoon). Increased monitoring and additional research is required and management plans should address the elasmobranch bycatch and in particular their high mortality rate.
Neurocognitive mechanisms of reactions to second- and third-party justice violations
Authors: Civai Claudia , Huijsmans Inge , Sanfey Alan G. 
Published: June 2019

AbstractThe aim of the current study was threefold: (i) understand people’s willingness to engage in either punishment of the perpetrator or compensation of the victim in order to counteract injustice; (ii) look into the differences between victims of and witnesses to injustice; (iii) investigate the different role played by social preference and affective experience in determining these choices. The sample tested here showed an equal preference for punishment and compensation; neuroimaging findings suggested that compensation, as opposed to punishment, was related to Theory of Mind. Partially supporting previous literature, choosing how to react to an injustice as victims, rather than witnesses, triggered a stronger affective response (striatal and prefrontal activation). Moreover, results supported the idea that deciding whether or not to react to an injustice and then how severely to react are two distinct decisional stages underpinned by different neurocognitive mechanisms, i.e., sensitivity to unfairness (anterior insula) and negative affectivity (amygdala). These findings provide a fine-grained description of the psychological mechanisms underlying important aspects of social norm compliance.
Episodic tremor and slip silently invades strongly locked megathrust in the Nankai Trough
Authors: Kano Masayuki , Kato Aitaro , Obara Kazushige 
Published: June 2019

AbstractRecent seismic and geodetic observations in subduction zones have revealed that slow earthquakes have preceded some large earthquakes. Characterization of slow earthquakes and their relation to large earthquakes provides important clues to constrain a wide spectrum of slip rates on tectonic faults. Here, we report new evidence of a slow slip transient at the downdip edge of the strongly locked seismogenic zone in the western Nankai Trough in southwest Japan. This slow slip transient was excited during an episodic tremor and slip at the downdip extension of the locked zone. Through this triggering, the frequent occurrence of the deep episodic tremor and slip invades the strongly locked megathrust zone and may intermittently increase the probability of large earthquakes in the Nankai Trough.
Inhibition of the Receptor for Advanced Glycation End-Products in Acute Respiratory Distress Syndrome: A Randomised Laboratory Trial in Piglets
Authors: Audard Jules , Godet Thomas , Blondonnet Raiko , Joffredo Jean-Baptiste , Paquette Bertille , Belville Corinne , Lavergne Marilyne , Gross Christelle , Pasteur Justine , Bouvier Damien , Blanchon Loic , Sapin Vincent , Pereira Bruno , Constantin Jean-Michel , Jabaudon Matthieu 
Published: June 2019

AbstractThe receptor for advanced glycation end-products (RAGE) modulates the pathogenesis of acute respiratory distress syndrome (ARDS). RAGE inhibition attenuated lung injury and restored alveolar fluid clearance (AFC) in a mouse model of ARDS. However, clinical translation will require assessment of this strategy in larger animals. Forty-eight anaesthetised Landrace piglets were randomised into a control group and three treatment groups. Animals allocated to treatment groups underwent orotracheal instillation of hydrochloric acid (i) alone; (ii) in combination with intravenous administration of a RAGE antagonist peptide (RAP), or (iii) recombinant soluble (s)RAGE. The primary outcome was net AFC at 4 h. Arterial oxygenation was assessed hourly and alveolar-capillary permeability, alveolar inflammation and lung histology were assessed at 4 h. Treatment with either RAP or sRAGE improved net AFC (median [interquartile range], 21.2 [18.8–21.7] and 19.5 [17.1–21.5] %/h, respectively, versus 12.6 [3.2–18.8] %/h in injured, untreated controls), oxygenation and decreased alveolar inflammation and histological evidence of tissue injury after ARDS. These findings suggest that RAGE inhibition restored AFC and attenuated lung injury in a piglet model of acid-induced ARDS.
Diversity and distribution of Laonice species (Annelida: Spionidae) in the tropical North Atlantic and Puerto Rico Trench
Authors: Guggolz Theresa , Meißner Karin , Schwentner Martin , Brandt Angelika 
Published: June 2019

AbstractLaoniceMalmgren, 1867 (Annelida: Spionidae) is a common polychaete genus in the deep-sea. Although most species are quite well studied morphologically, fragmentation and other damage that occurs during sampling often hampers morphological species identification of deep-sea specimens. In this study, we employ three molecular markers (16S, COI and 18S) to study the biodiversity and the distribution patterns ofLaonicefrom the tropical North Atlantic and the Puerto Rico Trench. Based upon different molecular analyses (Automated Barcode Gap Discovery, pairwise genetic distances, phylogenetics, haplotype networks) we were able to identify and differentiate eightLaonicespecies. Up to four of these species co-occurred sympatrically at the same station. The majority of species were found at multiple stations and two species in the eastern as well as western Atlantic had ranges of up to 4,000 km. Genetic differentiation across these extensive geographic distances was very low. Surprisingly, one 16S haplotype was shared between individuals 2,776 km apart and individuals from the Caribbean and the abyssal plain in the eastern Atlantic (>3,389 km) differed in only a single mutation in 16S. Our results suggest that members of this genus successfully disperse across large geographic distances and are largely unaffected by topographic barriers.
Real-world heart rate norms in the Health eHeart study
Authors: Avram Robert , Tison Geoffrey H. , Aschbacher Kirstin , Kuhar Peter , Vittinghoff Eric , Butzner Michael , Runge Ryan , Wu Nancy , Pletcher Mark J. , Marcus Gregory M. , Olgin Jeffrey 
Published: June 2019

AbstractEmerging technology allows patients to measure and record their heart rate (HR) remotely by photoplethysmography (PPG) using smart devices like smartphones. However, the validity and expected distribution of such measurements are unclear, making it difficult for physicians to help patients interpret real-world, remote and on-demand HR measurements. Our goal was to validate HR-PPG, measured using a smartphone app, against HR-electrocardiogram (ECG) measurements and describe out-of-clinic, real-world, HR-PPG values according to age, demographics, body mass index, physical activity level, and disease. To validate the measurements, we obtained simultaneous HR-PPG and HR-ECG in 50 consecutive patients at our cardiology clinic. We then used data from participants enrolled in the Health eHeart cohort between 1 April 2014 and 30 April 2018 to derive real-world norms of HR-PPG according to demographics and medical conditions. HR-PPG and HR-ECG were highly correlated (Intraclass correlation = 0.90). A total of 66,788 Health eHeart Study participants contributed 3,144,332 HR-PPG measurements. The mean real-world HR was 79.1 bpm ± 14.5. The 95th percentile of real-world HR was ≤110 in individuals aged 18–45, ≤100 in those aged 45–60 and ≤95 bpm in individuals older than 60 years old. In multivariable linear regression, the number of medical conditions, female gender, increasing body mass index, and being Hispanic was associated with an increased HR, whereas increasing age was associated with a reduced HR. Our study provides the largest real-world norms for remotely obtained, real-world HR according to various strata and they may help physicians interpret and engage with patients presenting such data.
Gamma-ray glow preceding downward terrestrial gamma-ray flash
Authors: Wada Yuuki , Enoto Teruaki , Nakamura Yoshitaka , Furuta Yoshihiro , Yuasa Takayuki , Nakazawa Kazuhiro , Morimoto Takeshi , Sato Mitsuteru , Matsumoto Takahiro , Yonetoku Daisuke , Sawano Tatsuya , Sakai Hideo , Kamogawa Masashi , Ushio Tomoo , Makishima Kazuo , Tsuchiya Harufumi 
Published: June 2019

AbstractTwo types of high-energy events have been detected from thunderstorms. One is “terrestrial gamma-ray flashes” (TGFs), sub-millisecond emissions coinciding with lightning discharges. The other is minute-lasting “gamma-ray glows”. Although both phenomena are thought to originate from relativistic runaway electron avalanches in strong electric fields, the connection between them is not well understood. Here we report unequivocal simultaneous detection of a gamma-ray glow termination and a downward TGF, observed from the ground. During a winter thunderstorm in Japan on 9 January 2018, our detectors caught a gamma-ray glow, which moved for ~100 s with ambient wind, and then abruptly ceased with a lightning discharge. Simultaneously, the detectors observed photonuclear reactions triggered by a downward TGF, whose radio pulse was located within ~1 km from where the glow ceased. It is suggested that the highly-electrified region producing the glow was related to the initiation of the downward TGF.
Stochastic dynamics and pattern formation of geometrically confined skyrmions
Authors: Schäffer Alexander F. , Rózsa Levente , Berakdar Jamal , Vedmedenko Elena Y. , Wiesendanger Roland 
Published: June 2019

AbstractEnsembles of magnetic skyrmions in confined geometries are shown to exhibit thermally driven motion on two different time scales. The intrinsic fluctuating dynamics (t ∼ 1 ps) are governed by short-range symmetric and antisymmetric exchange interactions, whereas the long-time limit (t ≳ 10 ns) is determined by the coaction of skyrmion–skyrmion-repulsion and the system’s geometry. Micromagnetic simulations for realistic island shapes and sizes are performed and analyzed, indicating the special importance of skyrmion dynamics at finite temperatures. We demonstrate how the competition between skyrmion mobility and observation time directly affects the addressability of skyrmionic bits, which is a key challenge on the path of developing skyrmion-based room-temperature applications. The presented quasiparticle Monte Carlo approach offers a computationally efficient description of the diffusive motion of skyrmion ensembles in confined geometries, like racetrack memory setups.
Abundances of sulphur molecules in the Horsehead nebula
Authors: Rivière-Marichalar P. , Fuente A. , Goicoechea J. R. , Pety J. , Le Gal R. , Gratier P. , Guzmán V. , Roueff E. , Loison J. C. , Wakelam V. , Gerin M. 
Published: June 2019

Context. Sulphur is one of the most abundant elements in the Universe (S/H ~ 1.3 × 10−5) and plays a crucial role in biological systems on Earth. The understanding of its chemistry is therefore of major importance.
Aims. Our goal is to complete the inventory of S-bearing molecules and their abundances in the prototypical photodissociation region (PDR) the Horsehead nebula to gain insight into sulphur chemistry in UV irradiated regions. Based on the WHISPER (Wide-band High-resolution Iram-30 m Surveys at two positions with Emir Receivers) millimeter (mm) line survey, our goal is to provide an improved and more accurate description of sulphur species and their abundances towards the core and PDR positions in the Horsehead.
Methods. The Monte Carlo Markov chain (MCMC) methodology and the molecular excitation and radiative transfer code RADEX were used to explore the parameter space and determine physical conditions and beam-averaged molecular abundances.
Results. A total of 13 S-bearing species (CS, SO, SO2, OCS, H2CS – both ortho and para – HDCS, C2S, HCS+, SO+, H2S, S2H, NS and NS+) have been detected in the two targeted positions. This is the first detection of SO+ in the Horsehead and the first detection of NS+ in any PDR. We find a differentiated chemical behaviour between C–S and O–S bearing species within the nebula. The C–S bearing species C2S and o-H2CS present fractional abundances a factor of > two higher in the core than in the PDR. In contrast, the O–S bearing molecules SO, SO2, and OCS present similar abundances towards both positions. A few molecules, SO+, NS, and NS+, are more abundant towards the PDR than towards the core, and could be considered as PDR tracers.
Conclusions. This is the first complete study of S-bearing species towards a PDR. Our study shows that CS, SO, and H2S are the most abundant S-bearing molecules in the PDR with abundances of approximately a few 10−9. We recall that SH, SH+, S, and S+ are not observable at the wavelengths covered by the WHISPER survey. At the spatial scale of our observations, the total abundance of S atoms locked in the detected species is <10−8, only ~0.1% of the cosmic sulphur abundance.
Internal Mammary Lymph Node Visualization as a Sentinel Sonographic Sign of Tuberculous Pleurisy
Authors: Messineo Ludovico , Quadri Federico , Valsecchi Alberto , Lonni Sara , Palmiotti Antonio , Giampietro Marchetti 
Published: June 2019

Abstract
Background Tuberculous pleurisy is one of the primary sites of extrapulmonary tuberculosis, but clinicians currently lack the diagnostic tools necessary for early recognition in the absence of typical signs and symptoms. With this study, we aimed to test the association between internal mammary adenopathies and tuberculous pleurisy (TP).
Methods 60 patients with a post-thoracoscopic histological diagnosis of granulomatosis or acute infective pleurisy were retrospectively enrolled. All of them had chest sonography and/or CT scan data available. At least two expert chest sonography physicians re-analyzed the sonography images to look for any internal mammary adenopathy. Such findings were compared to the CT data.
Results Chest sonography showed internal mammary adenopathy ipsilateral to the pleural effusion in 97 % of 29 patients who had a diagnosis of TP, and in 13 % of those with an acute infective pleurisy (p < 0.001). Receiver operator characteristic analysis revealed 97 % sensitivity and 87 % specificity for this technique in predicting TP (area under curve 0.92 ± 0.04, p < 0.001). CT detection power and node measures were significantly similar (p < 0.001).
Conclusion Sonographic internal mammary node visualization ipsilateral to the pleural effusion may become a sentinel sign for TP, contributing to early diagnosis or orienting the diagnostic management towards invasive procedures in uncertain cases.