bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lectin isolated from Bothrops jararacussu venom induces IL-10 release by TCD4+ cells and TNF-α release by monocytes and natural killer cells
Authors: Pires Weverson Luciano , Kayano Anderson Makoto , de Castro Onassis Boeri , Paloschi Mauro Valentino , Lopes Jéssica Amaral , Boeno Charles Nunes , Pereira Soraya dos Santos , Antunes Maísa Mota , Rodrigues Moreno Magalhães S , Stábeli Rodrigo Guerino , Fernandes Carla Freire Celêdonio , Soares Andreimar Martins , Zuliani Juliana Pavan 
Published: May 2019

Abstract
BjcuL is a C-type lectin isolated from Bothrops jararacussu snake venom with specificity for binding β-d-galactose units. BjcuL is not toxic to human peripheral blood mononuclear cells (PBMCs), but it inhibits PBMC proliferation and stimulates these cells to produce superoxide anions and hydrogen peroxide primarily via lymphocyte stimulation; it does not stimulate the production of nitric oxide and PGE2. The purpose of this study was to investigate the effect of BjcuL on PBMC activation with a focus on cytokine release modulating PBMC proliferation. The results showed for the first time that BjcuL coupled to FITC interacted with monocytes, B cells, natural killer (NK) cells, and with subpopulations of T cells. These cell-cell interactions can lead to cell activation and inflammatory cytokines release, such as IL-6 and TNF-α, as well as the anti-inflammatory cytokine IL-10. In addition, TNF-α release was attributed to NK cells and monocytes, whereas IL-10 was attributed to TCD4+ and Treg cells when stimulated by BjcuL. The temporal cytokines profile produced by cells when stimulated with this lectin allows us to assert that BjcuL has immunomodulatory activity in this context.
Increased frequencies of circulating CCR5+ memory T cells are correlated to chronic chagasic cardiomyopathy progression
Authors: Roffe Ester , Dos Santos Luara I , Santos Maykon O , Henriques Priscilla M , Teixeira-Carvalho Andrea , Martins-Filho Olindo A , Rocha Manoel O C , Eloi-Santos Silvana M , Correa-Oliveira Rodrigo , Antonelli Lis R V 
Published: May 2019

Abstract
The infection with the protozoan parasite Trypanosoma cruzi causes Chagas disease, a neglected tropical disease in Latin America and an imported emerging disease worldwide. Chronic Chagasic cardiomyopathy (CCC), a progressive inflammatory and fibrosing disease, is the most prominent clinical form of Chagas disease, culminating in heart failure and high rates of sudden death. CCC pathogenesis is influenced by both host and parasite factors and is proposed to be mostly immune-driven. Chemokines are crucial players in orchestrating immune cell recruitment to infected tissues and inflammation. Herein, we investigated inflammatory chemokine receptor expression on circulating T cells in patients stratified by CCC severity. Compared to asymptomatic individuals, we found increased percentages of effector CD4+ T cells and central memory CD4+ and CD8+ T cells expressing CCR5 in patients with structural cardiopathy, but normal global ventricular function and no symptoms of chronic heart failure. Even naïve T cells expressed CCR5 in these patients. In contrast, reduced frequencies of CD4+ and CD8+ effector T cells expressing CXCR3 were observed in patients presenting with severe heart disease. Patients with increased left ventricular diameter, heart enlargement, and insufficiency had higher frequencies of CCR5+ effector and effector memory CD8+ T cells. Moreover, the percentage of effector CCR5+ CD8+ T cells was increased in patients with a reduced ejection fraction. Our results show that high expression CCR5 and low expression of CXCR3 on circulating T cells are associated with worse prognosis, possibly reflecting immune-mediated cardiac remodeling of CCC.
Assessing Tumor Mechanics by MR Elastography at Different Strain Levels
Authors: Pagé Gwenaël , Tardieu Marion , Besret Laurent , Blot Lydia , Lopes Joaquim , Sinkus Ralph , Van Beers Bernard E. , Garteiser Philippe 
Published: May 2019

BackgroundMalignant tumors are associated with increased tissue rigidity, which can be an indicator of tumor progression. MR elastography (MRE) has the potential to study the variations of tumor mechanical properties. ex vivo studies have shown the ability of MRE to assess increase of mechanical properties; nevertheless, it has not yet been observed in vivo.PurposeTo propose a method to assess the increase in mechanical properties of tumors in vivo under static external compression using MRE.Study TypeProspective, experimental study.Animal ModelForty‐six SCID mice with subcutaneous tumor implantation (patient‐derived hepatocellular carcinoma xenografts, Model 1, n = 13, and Model 2, n = 33).Field Strength/Sequence7.0T; a spin echo sequence was used for anatomical images and a modified spin echo sequence for elastography acquisitions with a vibration frequency of 600 Hz.AssessmentAn inflatable balloon was placed on the abdomen to apply a load to the tumor. MRE acquisitions were performed at the basal state and at increasing compression levels. Anatomical images were used to calculate the octahedral shear strain between the tumor at the basal strain state and each strain level. For six mice (Model 2), each static preloading scan was acquired twice consecutively without moving the mouse to evaluate repeatability.Statistical Tests: The Bland–Altman method was used to assess repeatability. Correlations between tumor stiffness and deformation were evaluated with Pearson correlation coefficients.ResultsFor stiffness (G*), a good repeatability was obtained between the acquisitions; the limits of agreement of the Bland–Altman test were [–10.17%; 11.49%] with an absolute bias of 0.66%. A significant correlation between tumor stiffness and deformation was observed for both models (Model 1: r = 0.57, P < 0.0001 and Model 2: r = 0.31, P < 0.0001).Data ConclusionWe establish that tumor mechanical properties can increase under mechanical compression. This increase can effectively be monitored using a proposed MRE setup.Level of Evidence: 2Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2019;50:1982–1989.
Integrated care for methadone maintenance patients with hepatitis C virus infection
Authors: Tai Chi‐Ming , Yen Yung‐Chieh , Bair Ming‐Jong , Tseng Cheng‐Hao , Chang Ting‐Ting , Huang Chung‐Feng , Yeh Ming‐Lun , Dai Chia‐Yen , Chuang Wan‐Long , Yu Ming‐Lung , Huang Jee‐Fu 
Published: May 2019

AbstractThe majority of patients undergoing methadone maintenance treatment (MMT) are neither examined nor treated for hepatitis C virus (HCV) infection. We aimed to evaluate an integrated referral model in the management of HCV among MMT patients. This retrospective study included 390 HCV‐infected MMT patients between April 2015 and May 2017. Patients who tested positive for HCV antibodies were referred to a liver clinic by MMT case managers or psychiatrists. Patients who agreed to receive anti‐HCV treatment were treated with pegylated interferon and ribavirin. The rate of patient engagement at a liver clinic increased from 14.1% to 58.2% after integrated care. Multiple logistic regression analysis showed that higher education level (odds ratio [OR], 1.62; 95% confidence interval [CI], 1.01‐2.60) and elevated ALT level (OR, 4.30; 95% CI, 2.70‐6.85) were independently associated with patients who accepted referral. Active drug use (OR, 0.52; 95% CI, 0.31‐0.85) was inversely associated with referral acceptance. Of the 112 patients who met the criteria for anti‐HCV therapy, 66 (58.9%) were treated with pegylated interferon and ribavirin. Finally, the rate of treatment completion and sustained virological response (SVR) was 65.2% and 54.5%, respectively, among the 66 patients. Treatment completion (OR, 39.67; 95% CI, 7.80‐201.62) was found to be the only independent factor associated with SVR achievement. Although integrated care by psychiatrists and hepatologists significantly increased the rates of engagement and acceptance of antiviral treatment for HCV‐infected MMT patients, only a minority of MMT patients achieved successful treatment.
Lipid Profile and Glycerophospholipid Molecular Species in Two Species of Edible Razor Clams Sinonovacula constricta and Solen gouldi
Authors: Wu Zi‐Xuan , Zhou Da‐Yong , Zhao Man‐Tong , Xie Hong‐Kai , Liu Zhong‐Yuan , Liu Yan‐Fei , Liu Yu‐Xin , Shahidi Fereidoon 
Published: May 2019

AbstractTotal lipids were extracted from razor clams Sinonovacula constricta and Solen gouldi, and the molecular species of glycerophospholipid (Gpl) including choline glycerophospholipid (ChoGpl), ethanolamine glycerophospholipid (EtnGpl), serine glycerophospholipid (SerGpl), inositol glycerophospholipid (InsGpl), lysoChoGpl, lysoEtnGpl, and lysoSerGpl were characterized using a direct‐infusion tandem mass spectrometric method for the first time. Meanwhile, the lipid class composition and phospholipid (PL) class composition as well as the fatty acid (FA) composition of total lipids, triacylglycerol (TAG), and PL were also investigated. About 238 and 235 molecular species were characterized, respectively, in Sinonovacula constricta and Solen gouldi. The majority of the dominant Gpl molecular species contained n‐3 long‐chain polyunsaturated fatty acids (n‐3 LC‐PUFA), especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). Also, razor clam lipids contained a high‐proportioned PL (52.19–65.41% of total lipids) and PUFA (47.94–54.81 mol%). Furthermore, PL contained a higher proportion of PUFA (63.05–67.13 mol%), especially DHA (20.04–22.47 mol%) and EPA (16.27–21.46 mol%) than TAG (the corresponding values being 33.73–34.45, 11.95–12.27, and 8.13–0.8.99 mol%, respectively). Meanwhile, phosphatidylcholine (44.38–46.21 mol%) and phosphatidylethanolamine (38.84–39.95 mol%) were dominant among PL. In consideration of the high proportion of PUFA‐enriched Gpl, razor clam plays a great role in promoting human health.
Scaling of spectra of a class of self‐similar measures on R
Authors: Wang Zhi‐Min , Dong Xin‐Han , Ai Wen‐Hui 
Published: May 2019

AbstractLet be two positive integers. For , let the self‐similar measure be defined by . It is known [18] that is a spectral measure with a spectrum
urn:x-wiley:0025584X:media:mana201800360:mana201800360-math-0006where . In this paper, we give some conditions on under which the scaling set is also a spectrum of .
Transcriptional profiling of long noncoding RNAs and their target transcripts in ovarian cortical tissues from women with normal menstrual cycles and primary ovarian insufficiency
Authors: Yao Guidong , He Jiahuan , Kong Yue , Zhai Jun , Xu Yijiang , Yang Guang , Kong Deqi , Dong Fangli , Shi Senlin , Yang Qingling , Sun Yingpu 
Published: May 2019

AbstractPrevious studies have shown that long noncoding RNAs (lncRNAs) show a highly tissue‐ and disease‐specific expression pattern and that they regulate the expression of neighboring genes. Because lncRNAs have been shown to be secreted into the general circulation, they may be used as diagnostic tools for some diseases. Primary ovarian insufficiency (POI) is a disease in which women have menstrual cessation before the age of 40, accompanied by elevated follicle stimulating hormone and decreased estrogen levels. In this study, ovarian cortical tissues from five women with normal menstrual cycles and from five POI patients were used for next‐generation RNA sequencing. We found 20 differentially expressed lncRNAs with 12 upregulated and eight downregulated lncRNAs in cortical tissues of POI ovaries, compared with normal controls (fold change ≥ 2 and false discovery rate[FDR] ≤ 0.05). We also found 52 differentially expressed messenger RNA transcripts, with 33 upregulated and 19 downregulated ones (foldchange ≥ 2 and FDR ≤ 0.05). Functional annotation showed that these differentially expressed transcripts were associated with follicular development and granulosa cell function. Thirteen differentially expressed lncRNAs and their targeted neighboring transcripts were coregulated in ovarian cortical tissues, including lnc‐ADAMTS1–1:1/ADAMTS1, lnc‐PHLDA3–3:2/CSRP1, lnc‐COL1A1–5:1/COL1A1, lnc‐SAMD14–5:3/COL1A1, and lnc‐GULP1–2:1/COL3A1. Furthermore, serum levels of these lncRNAs in POI patients were significantly different from those in normal patients (
p < 0.05), and expression differences were consistent with those in ovarian cortical tissues. This study showed that key lncRNAs were differentially expressed in both ovarian cortical tissues and serum samples between women with normal menstrual cycles and POI patients. Further studies on the regulation of ovarian lncRNAs during follicular development are critical in understanding the etiologies of POI. Analyses of lncRNA expression in serum samples might provide a basis for early diagnosis and treatment of POI.
Cyclooxygenase‐2 is acutely induced by CCAAT/enhancer‐binding protein β to produce prostaglandin E2 and F2α following gonadotropin stimulation in Leydig cells
Authors: Yazawa Takashi , Imamichi Yoshitaka , Yuhki Koh‐ichi , Uwada Junsuke , Mikami Daisuke , Shimada Masayuki , Miyamoto Kaoru , Kitano Takeshi , Takahashi Satoru , Sekiguchi Toshio , Suzuki Nobuo , Rafiqul Islam Khan Md. , Ushikubi Fumitaka , Umezawa Akihiro , Taniguchi Takanobu 
Published: May 2019

AbstractCyclooxygenase 2 (COX‐2) is an inducible rate‐limiting enzyme for prostanoid production. Because COX‐2 represents one of the inducible genes in mouse mesenchymal stem cells upon differentiation into Leydig cells, we investigated COX‐2 expression and production of prostaglandin (PG) in Leydig cells. Although COX‐2 was undetectable in mouse testis, it was transiently induced in Leydig cells by human chorionic gonadotropin (hCG) administration. Consistent with the finding that Leydig cells expressed aldo‐keto reductase 1B7 (PGF synthase) and PGE synthase 2, induction of COX‐2 by hCG caused a marked increase in testicular PGF
2α and PGE
2 levels. Using mouse Leydig cell tumor‐derived MA‐10 cells as a model, it was indicated by reporter assays and electron mobility shift assays that transcription of the COX‐2 gene was activated by CCAAT/enhancer‐binding protein β (C/EBPβ) with cAMP‐stimulation. C/EBPβ expression was induced by cAMP‐stimulation, whereas expression of C/EBP homolog protein (CHOP) was robustly downregulated. Transfection of CHOP expression plasmid inhibited cAMP‐induced COX‐2 promoter activity. In addition, CHOP reduced constitutive COX‐2 expression in other mouse Leydig cell tumor‐derived TM3 cells. These results indicate that COX‐2 is induced in Leydig cells by activation of C/EBPβ via reduction of CHOP expression upon gonadotropin‐stimulation to produce PGF
2α and PGE
2.
Effects of GABA and Leptin Receptor‐Expressing Neurons in the Lateral Hypothalamus on Feeding, Locomotion, and Thermogenesis
Authors: de Vrind Véronne A.J. , Rozeboom Annemieke , Wolterink‐Donselaar Inge G. , Luijendijk‐Berg Mieneke C.M. , Adan Roger A.H. 
Published: May 2019

ObjectiveThe lateral hypothalamus (LH) is known for its role in feeding, and it also regulates other aspects of energy homeostasis. How genetically defined LH neuronal subpopulations mediate LH effects on energy homeostasis remains poorly understood. The behavioral effects of chemogenetically activating LH gamma‐aminobutyric acid (GABA) and the more selective population of LH GABA neurons that coexpress the leptin receptor (LepR) were compared.MethodsLepR‐cre and VGAT‐cre mice were injected with AAV5‐hSyn‐DIO‐hM3DGq‐mCherry in the LH. The behavioral effects of LH GABA or LH LepR neuronal activation on feeding, locomotion, thermogenesis, and body weight were assessed.ResultsThe activation of LH GABA neurons increased body temperature (P ≤ 0.008) and decreased body weight (P ≤ 0.01) despite decreased locomotor activity (P = 0.03) and transiently increased chow intake (P ≤ 0.009). Also, similar to other studies, this study found that activation of LH GABA neurons induced gnawing on both food and nonfood (P = 0.001) items. Activation of LH LepR neurons decreased body weight (P ≤ 0.01) and chow intake when presented on the cage floor (P ≤ 0.04) but not when presented in the cage top and increased locomotor activity (P = 0.002) and body temperature (P = 0.03).ConclusionsLH LepR neurons are a subset of LH GABA neurons, and LH LepR activation more specifically regulates energy homeostasis to promote a negative energy balance.
Determinants of satisfactory patient communication and shared decision making in patients with multiple myeloma
Authors: Nejati Babak , Lin Chien‐Chin , Aaronson Neil K. , Cheng Andy S.K. , Browall Maria , Lin Chung‐Ying , Broström Anders , Pakpour Amir H. 
Published: May 2019

AbstractObjectiveTo identify determinants of shared decision making in patients with multiple myeloma (MM) to facilitate the design of a program to maximize the effects of shared decision making.MethodsThis prospective longitudinal study recruited 276 adult patients (52% male, mean age 62.86 y, SD 15.45). Each patient completed the eHealth Literacy Scale (eHEALS), Multidimensional Trust in Health Care Systems Scale (MTHCSS), Patient Communication Pattern Scale (PCPS), and 9‐Item Shared Decision‐Making Questionnaire (SDM‐Q‐9) at baseline and the SDM‐Q‐9 again 6 months later. One family member of the patient completed the Family Decision‐Making Self‐Efficacy (FDMSE) at baseline. Structural equation modeling (SEM) was used to investigate the associations between eHealth literacy (eHEALS), trust in the health care system (MTHCSS), self‐efficacy in family decision making (FDMSE), patient communication pattern (PCPS), and shared decision making (SDM‐Q‐9).ResultsSEM showed satisfactory fit (comparative fit index = 0.988) and significant correlations between the following: eHealth literacy and trust in the health care system (β = 0.723, P < 0.001); eHealth literacy and patient communication pattern (β = 0.242, P < 0.001); trust in the health care system and patient communication pattern (β = 0.397, P < 0.001); self‐efficacy in family decision making and patient communication pattern (β = 0.264, P < 0.001); eHealth literacy and shared decision making (β = 0.267, P < 0.001); and patient communication pattern and shared decision making (β = 0.349, P < 0.001).ConclusionsPatient communication and eHealth literacy were found to be important determinants of shared decision making. These factors should be taken into consideration when developing strategies to enhance the level of shared decision making.
Electrospinning Synthesis of Porous NiCoO2 Nanofibers as High‐Performance Anode for Lithium‐Ion Batteries
Authors: Wang Jinkai , Xie Sanmu , Li Li , Li Zhihui , Asiri Abdullah M. , Marwani Hadi M. , Han Xiaogang , Wang Hongkang 
Published: May 2019

AbstractNanostructured ternary/mixed transition metal oxides have attracted considerable attentions because of their high‐capacity and high‐rate capability in the electrochemical energy storage applications, but facile large‐scale fabrication with desired nanostructures still remains a great challenge. To overcome this, a facile synthesis of porous NiCoO2 nanofibers composed of interconnected nanoparticles via an electrospinning–annealing strategy is reported herein. When examined as anode materials for lithium‐ion batteries, the as‐prepared porous NiCoO2 nanofibers demonstrate superior lithium storage properties, delivering a high discharge capacity of 945 mA h g−1 after 140 cycles at 100 mA g−1 and a high rate capacity of 523 mA h g−1 at 2000 mA g−1. This excellent electrochemical performance could be ascribed to the novel hierarchical nanoparticle‐nanofiber assembly structure, which can not only buffer the volumetric changes upon lithiation/delithiation processes but also provide enlarged surface sites for lithium storage and facilitate the charge/electrolyte diffusion. Notably, a facile synthetic strategy for fabrication of ternary/mixed metal oxides with 1D nanostructures, which is promising for energy‐related applications, is provided.
The economic impacts of blackbird (Icteridae) damage to sunflower in the USA
Authors: Ernst Karina , Elser Julie , Linz George , Kandel Hans , Holderieath Jason , DeGroot Samantha , Shwiff Steven , Shwiff Stephanie 
Published: May 2019

AbstractBACKGROUNDBlackbird (Icteridae) damage to ripening sunflower (Helianthus annuus L.) has been a persistent economic issue in the USA for the last five decades. To quantify losses, we surveyed blackbird damage from 2001 to 2013 (excluding 2004) to physiologically mature sunflower in eight states: North Dakota, South Dakota, Texas, Nebraska, Minnesota, Colorado, Kansas, and Vermont.RESULTSWe pooled data gathered during the most recent 5 years (2009 to 2013) of the survey and found losses averaged $US2.5 million and $US11.3 million for confectionery and oilseed hybrids, respectively. Three states, North Dakota, South Dakota, and Nebraska, had sufficient acreage and bird damage to warrant economic analyses using a regional economic model. The average annual total (direct plus indirect) economic impact of bird damage to sunflower production for North Dakota, South Dakota, and Nebraska was $US18.7 million, $US7.3 million, and $US2.6 million, respectively.CONCLUSIONThis study provides a better understanding of the broader economic implications of bird damage to sunflower by estimating the macroeconomic impacts of lost sunflower production in the region. Additionally, the findings of this study may be used to inform decisions regarding bird management policy by providing policymakers with information to determine the downstream value of bird management and the possible benefits of improved methods that further mitigate losses. © 2019 Society of Chemical Industry
Physiologically‐Based Pharmacokinetic Modeling of Fluconazole Using Plasma and Cerebrospinal Fluid Samples From Preterm and Term Infants
Authors: Gerhart Jacqueline G. , Watt Kevin M. , Edginton Andrea , Wade Kelly C. , Salerno Sara N. , Benjamin Daniel K. , Smith P. Brian , Hornik Christoph P. , Cohen‐Wolkowiez Michael , Duara Shahnaz , Ross Ashley , Shattuck Karen , Stewart Dan L. , Neu Natalie , Gonzalez Daniel ,   the Best Pharmaceuticals for Children Act—Pediatric Trials Network Steering Committee
Published: May 2019

Fluconazole is used to treat hematogenous Candida meningoencephalitis in preterm and term infants. To characterize plasma and central nervous system exposure, an adult fluconazole physiologically‐based pharmacokinetic (PBPK) model was scaled to infants, accounting for age dependencies in glomerular filtration and metabolism. The model was optimized using 760 plasma samples from 166 infants (median postmenstrual age (range) 28 weeks (24–50)) and 27 cerebrospinal fluid (CSF) samples from 22 infants (postmenstrual age 28 weeks (24–33)). Simulations evaluated achievement of the surrogate efficacy target of area under the unbound concentration‐time curve ≥ 400 mg • hour/L over the dosing interval in plasma and CSF using dosing guidelines. Average fold error of predicted concentrations was 0.73 and 1.14 for plasma and CSF, respectively. Target attainment in plasma and CSF was reached faster after incorporating a loading dose of 25 mg/kg. PBPK modeling can be useful in exploring CNS kinetics of drugs in children.
High Lithium‐Ion Storage Performance of Ti3SiC2 MAX by Oxygen Doping
Authors: Luan Sunrui , Zhou Junshuang , Xi Yaokai , Han Minze , Wang Dong , Gao Jiajia , Hou Li , Gao Faming 
Published: May 2019

AbstractIn this work, Ti3SiC2, layered ternary transition metal carbide called MAX phase, was invoked as the anode material for lithium‐ion batteries. Oxygen‐doped Ti3SiC2, named O‐Ti3SiC2, was produced by calcination in air. The initial reversible capacity was ∼70 mAh g−1 at 10 C, which increased to ∼180 mAh g−1 after 3000 cycles. The capacity was four times higher than original Ti3SiC2, showing a suitable electrochemical performance.