bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hidden messages in fluids: A review of clinical and fundamental perspectives on post‐lymph node dissection drains
Authors: Mak Chon‐Hou , Wang Guang‐Rui , Li Zi‐Zhan , Cao Lei‐Ming , Zhang Chen‐Xi , Zhu Zhao‐Qi , Liu Bing , Bu Lin‐Lin 
Published: October 2024

AbstractIn recent years, there has been a growing interest in liquid biopsy due to its non‐invasive diagnostic value. Postoperative drainage fluid (PDF) is the fluid exudate from the wound site following lymph node dissection. PDF is regarded as a medical waste with no specific clinical significance. Nevertheless, the liquid biopsy of PDF may enable the reuse of this fluid. PDF contains a variety of body fluids, including blood and lymph. PDF contains a variety of biological components, including cytokines, extracellular vesicles (EVs), proteins, nucleic acids, cells and bacteria. These components are indicative of the postoperative inflammatory response, the immune response and the therapeutic response. In this review, we examine the current state of research in the field of liquid biopsy in PDF, elucidating how the analysis of its components can assess the prognosis of patients after lymph node dissection, monitor real‐time changes in patient status, and identify new biomarkers and potential therapeutic targets.
The Role of Environment Social and Governance (ESG) Score To Cost of Debt: Evidence From ASEAN Countries*
Authors: Ariefianto Moch. Doddy , Rahmansyah Fitriani , Wijaya Valencia , Audreane Viary 
Published: October 2024

ABSTRACTEnvironmental social governance (ESG) disclosure has become an increasingly important component in determining a company's financing cost (cost of debt‐CoD). This phenomenon could be attributed to company reputation development and the rise of socially responsible investing. We contribute to the existing literature by investigating further the causality process of ESG scores to the reduction of CoD particularly in nonfinancial companies in one of the most dynamic world regions: Association of Southeast Asian Nations (ASEAN). To meet our research objective; we applied difference‐in‐difference analysis to a dataset of 135 nonfinancial companies (that published ESG score in Bloomberg) from 6 ASEAN countries, using annual frequency data from 2012 to 2021 (1350 observations). We found that ESG does reduce CoD in the range of 21.3% (measured by interest expense) to 27.7% (measured by credit spread). Our finding is quite solid under an array of robustness checks.
Visual Patterns of Diaphragmatic Motion in Acute Respiratory Failure: A Prospective Pilot Study
Authors: Markarian Thibaut , Daniel Matthieu , Guillemet Kevin , Ajavon Florian , Femy Florent , Grandpierre Romain Genre , Feral‐Pierssens Anne‐Laure , Bobbia Xavier 
Published: October 2024

ABSTRACTIntroductionRight diaphragmatic excursion is a reliable and reproducible technique used in intensive care to assess diaphragmatic function. The aim of this study was to investigate the relationship between the appearance of diaphragmatic motion and the etiological diagnosis of patients admitted to the emergency department with acute respiratory failure (ARF).MaterialsA prospective, observational, and multicenter pilot study was conducted. All adult patients admitted in the emergency department with ARF were included. The different visual patterns of diaphragmatic motion were analyzed according to the three main etiologies of ARF encountered in emergency departments.ResultsA total of 39 adult patients were included. We observed a different visual pattern in patients with pneumonia. A sum of plateau times of less than 0.2 s predicted that the main diagnosis was pneumonia, with sensitivity = 89% 95%CI [52%; 100%], specificity = 87% 95%CI [69%; 96%].ConclusionOur study seems to show that the shape of diaphragmatic motion in patients with ARF secondary to pneumonia is different from that in patients with exacerbation of chronic obstructive pulmonary disease or acute heart failure.Trial Registration: ClinicalTrials.gov: NCT04591509
Exploring the Soil Structure Analysis of a Subtropical Watershed by Introducing a Blanket Physico‐Hydraulic Health Index
Authors: Costa Angelo Garcia , Soares Mauricio Fornalski , Pinto Marília Alves Brito , Fuentes‐Guevara Miguel David , Nunes Rômulo Félix , Kaiser Douglas Rodrigo , Armindo Robson André , Timm Luis Carlos 
Published: October 2024

ABSTRACTMultiple disturbed and undisturbed soil samples were collected to assess the soil structure health in support of water use and management in a subtropical watershed, which is composed of shallow Entisols, near to Canguçu, RS state—Brazil. Various physico‐hydraulic properties related to soil structure, such as bulk density, total, micro and macroporosity, saturated hydraulic conductivity, organic carbon, soil water retention curve (SWRC), and the ranges of plant available water (PAWC) and drainable porosity (DP) were measured to be analyzed. The hydraulic‐energy indices of soil aeration and water retention were determined by integrating the areas under SWRC. Combining these properties with the contents of sand and clay, a unique blanket soil physico‐hydraulic health index (BSPHI) was formulated and applied to investigate the impact of land use and management on the soils of the Ellert Creek Watershed (ECW), thereby supporting soil physical health analysis and, consequently, the analysis of water and human health. The favorable outcomes found in the ECW soils under annual crops indicated good root aeration in the surface layer due to the balance between DP and PAWC. The BSPHI results showed that only about 19% of the areas exhibited unhealthy physical health. The soil structure in pasture areas of ECW was not significantly impacted by the extensive cattle grazing, whereas lands under native forest served as a control for the natural soil quality status. The BSPHI successfully captured small changes in soil structure, regardless of land use and management, highlighting its potential as a promising tool for evaluating soil health by assessing the soil physical quality.
Comprehensive single‐cell profiling of monocytes in HLA‐B27‐positive ankylosing spondylitis with acute anterior uveitis
Authors: Li Huan , Ju Xueming , Zhang Lixin , Zhu Jing , Zhang Jing , Xiao Jialing , Wang Ting , Wu Weijia , Wang Liang , Gan Chengzi , Li Xiangmei , Wei Yutong , Zhu Siyu , Zhou Yu , Deng Bolin , Xiao Ning , Gong Bo 
Published: October 2024

AbstractAcute anterior uveitis (AAU) is a common extra‐articular manifestation of ankylosing spondylitis (AS), particularly in patients positive for the human leucocyte antigen (HLA)‐B27 genetic marker. To explore the underlying mechanisms of HLA‐B27+ AS‐associated AAU, we employed single‐cell RNA sequencing to profile the transcriptomes of peripheral blood mononuclear cells in three HLA‐B27+ AS‐associated AAU patients and three healthy controls (HCs). We identified 11 distinct immune cell clusters, with a particular focus on monocytes, revealing six subsets, including three previously unidentified subsets, namely, GTPase immune‐associated proteins, Th17‐related, and lncRNA monocytes, with unique gene expression patterns. Significant differences in monocyte composition, activation states, and gene expression were observed between patients and HCs, particularly within HLA monocyte subpopulations. Notably, enhanced expression of X‐inactive specific transcript and myeloid cell nuclear differentiation antigen genes was validated across monocyte subclusters in patients. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis highlighted significant enrichment in antigen processing and presentation pathways, shedding light on the disease's molecular mechanisms. These findings provide novel insights into the molecular mechanisms of HLA‐B27+ AS‐associated AAU and may contribute to the development of targeted diagnostic and therapeutic strategies. Further clinical validation is essential.
Reduced intestinal‐to‐diffuse conversion and immunosuppressive responses underlie superiority of neoadjuvant immunochemotherapy in gastric adenocarcinoma
Authors: Wang Lei , Wan Linghong , Chen Xu , Gao Peng , Hou Yongying , Wu Linyu , Liu Wenkang , Tian Shuoran , Han Mengyi , Peng Shiyin , Tan Yuting , Pan Yuwei , Ren Yuanfeng , Li Jinyang , Wen Haihui , Liu Qin , Zhang Mengsi , Wang Tao , Qin Zhong‐Yi , Xiang Junyu , Chen Dongfeng , Li Xianfeng , Wang Shu‐Nan , Chen Chuan , Li Mengxia , Li Fan , Wang Zhenning , Wang Bin 
Published: October 2024

AbstractNeoadjuvant immunochemotherapy (NAIC) achieves superior clinical benefits over neoadjuvant chemotherapy (NAC) in multiple types of human cancers, including gastric adenocarcinoma (GAC). However, it is poorly understood how the malignant epithelial cells and tumor immune microenvironment (TIME) might respond distinctly to NAIC and NAC that underlies therapeutic efficacy. Here treatment‐naive and paired tumor tissues from multiple centers were subjected to pathological, immunological, and transcriptomic analysis. NAIC demonstrated significantly increased rate of pathological complete response compared to NAC (pCR: 25% vs. 4%, p < 0.05). Interestingly, pretreatment intestinal subtype of Lauren's classification was predictive of pathologic regression following NAIC, but not NAC. A substantial portion of cancers underwent intestinal‐to‐diffuse transition, which occurred less following NAIC and correlated with treatment failure. Moreover, NAIC prevented reprogramming to an immunosuppressive TIME with less active fibroblasts and exhausted CD8+ T cells, and increased numbers of mature tertiary lymphoid structures. Mechanistically, activation of the tumor necrosis factor alpha (TNFα)/nuclear factor‐kappa B (NF‐κB) signaling pathway was associated with response to NAIC. Together, NAIC is superior to NAC for locally advanced GAC, likely due to reduced intestinal‐to‐diffuse conversion and reprogramming to an immuno‐active TIME. Modulation of the histological conversion and immunosuppressive TIME could be translatable approaches to improve neoadjuvant therapeutic efficacy.
Bifidobacterium breve M‐16 V and scGOS/lcFOS Supplementation to Dams Ameliorates Infant Rotavirus Infection in Early Life
Authors: Sáez‐Fuertes Laura , Rio‐Aige Karla , Massot‐Cladera Malén , Castell Margarida , Knipping Karen , Garssen Johan , Bourdet‐Sicard Raphaëlle , Rodríguez‐Lagunas María José , Collado María Carmen , Pérez‐Cano Francisco José 
Published: October 2024

AbstractThe immune system of newborns is underdeveloped, leaving them susceptible to infections like rotavirus (RV). Despite vaccines, RV remains a leading cause of child mortality, especially in developing countries. Maternal immunity is transferred during pregnancy and breastfeeding to the offspring providing protection against RV infection. This study aims to explore how the maternal diet can enhance the newborn's ability to fight early infections. Pregnant rats received orally Bifidobacterium breve M‐16 V and short chain galacto‐oligosaccharides (scGOS)/long chain fructo‐oligosaccharides (lcFOS). At day 5 of life pups are infected with RV and at day 8, samples are collected for the infection analysis. Pups whose mothers received the synbiotic have lower RV infection severity. The levels of immunoglobulins (Ig) IgG2c and IgA are raised in pups' plasma and digested milk, respectively. Synbiotic supplementation improves intestinal maturation and increases gene expression of immune‐related genes. In conclusion, the administration of this synbiotic to gestating and lactating mothers ameliorates the incidence and severity of the pup's diarrhea caused by the RV infection by improving their immunity.
Protective Effect and Mechanism of L‐Theanine on Acute Alcoholic Liver Injury in Mice
Authors: Liu Meng‐Yuan , Xu Kai‐Hang , Liu Sha , Xiao Wen‐Jun 
Published: October 2024

AbstractScopeAcute alcoholic liver injury (AALI), a global health concern, is exacerbated by excessive episodic drinking. L‐theanine (LTA), a compound found in tea leaves, mitigates the AALI‐induced liver oxidative stress and inflammation. However, its relationship with alcohol metabolism and its liver‐protective mechanism remains unexplored.Methods and resultsThis study investigates the protective mechanisms of LTA against AALI in mice. The results demonstrate that LTA mitigates liver tissue damage and reduces the serum levels of aspartate aminotransferase and alanine aminotransferase, and liver levels of triglycerides, malondialdehyde, reactive oxygen species (ROS), tumor necrosis factor‐α, interleukin‐6, and interleukin‐1β. However, LTA enhances the activity of ethanol‐metabolizing enzymes and decreases ethanol and acetaldehyde serum levels. Mechanistically, LTA accelerates alcohol metabolism by upregulating the hepatic expression of ADH6, ALDH1B1, ALDH2, CAT, and ACSS1 mRNA and protein in AALI mice, LTA downregulates the expression of CYP2E1 mRNA and protein and promoting antioxidative activities thus reducing the accumulation of ROS. This attenuated inflammation by inhibiting the phosphorylation of nuclear factor‐kappa B inhibitor alpha (IκBα) and downregulating the hepatic expression of NF‐κB p65, TNF‐α, IL‐1β, IL‐6 mRNA, and protein.ConclusionLTA is a beneficial dietary supplement that protects against AALI by modulating alcohol metabolism and the TNF‐α/NF‐κB pathway.
Feasibility of a Community‐Based Aquatic and Peer Support Intervention for People With Musculoskeletal Disorders Delivered via a Cross‐Sector Partnership—A Service Evaluation
Authors: Wilson Nicky , Barcellona Massimo , Lambert Paul , Storey Petula , Foster Barrington , Waller Ben , Wilkins Ben 
Published: October 2024

ABSTRACTIntroductionWorldwide, the unmet rehabilitation needs of adults with musculoskeletal disorders are high. Aquatic exercise is clinically effective in managing musculoskeletal disorders, but access to rehabilitative services is limited.ObjectiveTo determine the feasibility of a community‐based aquatic exercise and peer support intervention for patients with musculoskeletal disorders delivered via a multisector partnership between an NHS physiotherapy service, a leisure operator and a social enterprise that creates personalised exercise programmes delivered through an application on a tablet computer. Cost‐sharing was implemented after an initial complimentary session.MethodsA mixed method service evaluation was undertaken. Patient demographics, attendance, and patient‐reported outcomes were summarised using descriptive statistics. Semi‐structured interviews and an online survey were employed to explore patient acceptability of the intervention.ResultsThirty four patients accessed the intervention during the 6‐month pilot. Over half were from minority ethnic groups and nearly two thirds were in the bottom three deciles relating to low income. Patients exercised in a group of up to eight, supported by NHS rehabilitation professionals and volunteers. Provision of post‐session refreshments encouraged connectivity between patients. Weekly attendance at the programme was high. Patient‐reported outcome measures showed favourable changes in pain intensity and function following the intervention. Qualitative data suggest that the intervention was acceptable to patients.ConclusionImplementation of a community‐based aquatic exercise and peer support intervention for people with musculoskeletal disorders via a cross‐sector partnership involving the NHS appears feasible and acceptable to patients. Further research is needed to assess the impact on musculoskeletal health and health inequalities.
Modelling the effect of point forces and moments in plate bending with hybrid‐Trefftz stress elements
Authors: Teixeira de Freitas J. A. , Tiago C. , Pereira E. M. B. R. 
Published: October 2024

AbstractThe formulation of the hybrid‐Trefftz stress element for plate bending is extended to the modelling of concentrated forces and moments, either as prescribed loads or as reactions at point supports. As the bending, torsion and shear fields are hypersingular, the flexibility matrix of the element involves the use of the finite part integration concept. In addition, it requires the confirmation of the positive‐definiteness of the flexibility under gross shape distortion. The tests illustrate the modelling of applied concentrated forces and moments and also the combination of boundary layer and point reaction effects. The results obtained are validated using converged solutions obtained with a stress‐based hybrid‐mixed element (HMS) and a displacement‐based mixed element (MITC).
At‐Line LC‐QTOF‐ESI‐MS/MS Fractionation of Impatiens balsamina Linn. Coupled With a Simple DPPH for Rapid Identification and Guided Isolation of Antioxidant
Authors: Srivilai Jukkarin , Nuengchamnong Nitra , Khorana Nantaka , Wisidsri Nakuntwalai , Thungmungmee Suradwadee , Aoonboontum Patteera , Sasea Krittanon , Suksathan Piyakaset , Rungsang Tammanoon , Ingkaninan Kornkanok , Mungmai Lapatrada 
Published: October 2024

ABSTRACTIntroductionReactive oxygen species (ROS) and ultraviolet (UV) light are significant factors to impair skin disorders. Impatiens balsamina Linn. (IB), a traditional Chinese and Thai herbal medicine, has long been used to treat skin and nail diseases, potentially due to its radical‐scavenging properties. However, specific antioxidant compounds in IB have not been well defined.ObjectiveThis work aims to rapidly identify, target, and isolate antioxidant biomarkers in IB using at‐line LC‐ESI‐QTOF‐(MS/MS) coupled with a simple DPPH assay and comprehensively investigate the antioxidant activities of IB extract and isolated biomarker.MethodologyFollowing liquid chromatography (LC), the eluent of IB extract was split into two streams (9:1 ratio). The majority was fractionated for DPPH assay in 96‐well plates, whereas 10% underwent chemical identification using ESI‐QTOF‐MS. Antioxidants in IB were identified, targeted, and promptly isolated through transfer from analytical LC to preparative HPLC. IB and the isolated biomarkers were evaluated for antioxidant effects using various antiradical assays and in suppressing ROS induced by UV in skin cells, keratinocytes, and fibroblasts.ResultsThirty‐one chemical constituents were identified, with four tentatively identified as potent antioxidants. Kaempferol emerged as a potential antioxidant biomarker in IB, exhibiting superior antioxidant activity in various in vitro assays compared with positive controls. Both IB extract and kaempferol effectively reduced UVB‐induced ROS in skin cells.ConclusionThis study represents the first comprehensive identification of antioxidants and chemical constituents in IB, pinpointing kaempferol as a key antioxidant biomarker. Its rapid identification using at‐line techniques holds promise for advancing bioactive compound discovery in herbal medicine.
DFT Computation, Spectroscopic, Hirshfeld Surface, Docking and Topological Analysis on 2,2,5‐Trimethyl‐1,3‐Dioxane‐5‐Carboxylic Acid as Potent Anti‐Cancer Agent
Authors: kumar J. Senthil , Karthik N. , Sumathi S. , Jyothi N. Siva , Saranya S. , Jeyavijayan S. 
Published: October 2024

ABSTRACTThe 2,2,5‐trimethyl‐1,3‐dioxane‐5‐carboxylic acid (TDCA) using both theoretical and experimental methods have been studied. The sample has been subjected to XRD, FTIR, FT‐Raman, (C13 and H1) NMR, and UV–vis spectrum analysis. Then, theoretical calculations have been performed at the DFT/B3LYP/6‐311++G(d,p) higher based scale. The theoretical and experimental geometrical parameters and frequencies have been compared well. Theoretical and experimental NMR chemical shifts have been determined. Absorption wavelengths of UV–Vis spectrum were experimentally measured and compared with TD‐DFT predictions. Detailed explanations have been given for frontier molecular orbitals, low density gradient, distribution of Mulliken charges, molecular electrostatic potential (MEP), RDG, localized orbital location, and electron localized activities. Based on the studied 2D image of the Hirschfield surfaces, H···H (65.6%) and O···H/H···O (33.6%) are found as the controlling interactions. A high binding affinity of −6.5 Kcal/mol has been calculated against 4OAR protein. These theoretical findings of the molecule may be used as an anticancer drug candidate, which helps to explain the structural stability, reactivity and anticancer potential of TDCA. High drug affinity for the TDCA has been detected by in silico ADMET prediction.
Leaving No One Behind: Examining the effect of financial, gender, and digital inclusion on sustainable development
Authors: Abdulkareem Hauwah K. K. , Jimoh Sodiq Olaiwola , Nofiu Taofeekat Temitope , Akande Rashidat Sumbola 
Published: October 2024

AbstractThis paper analyzes the effects of different dimensions of inclusion toward the attainment of sustainable development in 33 sub‐Saharan African (SSA) countries from the financial, gender, and technological perspectives between 2004 and 2021. This study employs the System Generalized Method of Moments given its ability to address potential endogeneity issues, capture the dynamic nature of relationships, and mitigate potential biases while adopting the Driscoll–Kraay standard errors for robustness check. Findings reveal two of the three measures of financial inclusion wield positive and significant effects on sustainable development while credit to the private sector is found to be detrimental. Also, all three measures of gender inclusion (female employment to population, female labor force participation rate, and the number of women holding a seat in parliament) positively influence sustainable development. Contrastingly, the coefficients of digital inclusion (mobile phone subscription and internet usage) were revealed to be harmful to sustainable development in SSA. This paper advocates for increased financial inclusion through financial literacy, customized financial products, and gender‐sensitive financial services. Pertinent are gender policies that address gender norms in addition to the enactment, avowed commitment, and support for affirmative action toward closing gender gaps across all sectors and at all levels.
Metal‐Organic Framework (Cu@ZIF) for Photocatalytic Reduction of p‐Nitrophenol and its Toxicological Evaluation using Zebrafish (Danio Rerio)
Authors: Lakshmi Krishnasamy , Kadirvelu Krishna 
Published: October 2024

AbstractGlobal food security depends on agricultural development, yet excessive fertilizer and pesticide usage is harmful to the environment. These substances have a role in human health problems, environmental changes, and erosion of the soil. Although p‐nitrophenol, an organic pollutant and other organophosphorous compounds are utilised in agriculture, their usage raises major health and environmental issues. These synthetic pesticides are quite effective at getting rid of pests, but they have also been linked to serious health problems for people and animals, degraded soil, and contaminated water. One typical persistent pollutant present in industrial wastewater is the organic pollutant, which is produced by organophosphorous compounds. The primary objective of this work is to use Cu@ZIF for photoreduction of p‐nitrophenol (PNP). A UV‐visible spectrophotometer test revealed that the deterioration peak after 60 mins. An analyzer for total organic carbon (TOC) was utilised to assess the PNP mineralization. The catalyst remained same and continued in the same behaviour even after four cycles. This study evaluated the acute toxicity of PNP on the development of early‐stage zebrafish, paying particular attention to the embryonic developmental deformities. The technique of photocatalytic decontamination is safe for the environment, financially practical, and technically achievable.