bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films
Authors: Hashemi Mahsa , Hashemi Mohammad , Amiri Elham , Hassanzadazar Hassan , Daneshamooz Shahrzad , Aminzare Majid 
Published: July 2019

Background:
Biodegradable films are appropriate alternatives to synthetic polymers due to
their potential to enhance food safety and their function as a carrier for wide a range of food additives
especially natural antioxidants.

Objective:
The aim of this study was to compare the potential antioxidant activities of sodium alginate
films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil
(ZEO) using an in vitro model.

Methods:
At first, the major chemical compositions of ZEO were evaluated by GC/MS. Then, the
amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay
and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and
2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Finally, the concentrations of
ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to
films in combination forms to evaluate their synergistic effects.

Results:
According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of
ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most
important compounds of essential oil, respectively. The results of the radical scavenging evaluations,
showed better antioxidant activities of the films containing RES than the films containing ZEO and
Butylated hydroxytoluene (BHT). Also, the combined uses of ZEO and RES into alginate films had the
synergistic effects (P<0.05).

Conclusion:
Therefore, it can be concluded that alginate films incorporated with the combination of
ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural
antioxidant in the food packaging industry.
Fabrication and in-vitro Investigation of Polycaprolactone - (Polyvinyl Alcohol/Collagen) Hybrid Nanofiber as Anti-Inflammatory Guided Tissue Regeneration Membrane
Authors: Limoee Mazdak , Moradipour Pouran , Godarzi Mahnaz , Arkan Elham , Behbood Leila 
Published: July 2019

Background:
Periodontal disease is the most common oral condition that affects the tissue
surrounding the teeth. The oral cavity is colonized by an impressive array of micro-organisms, many of
which can colonize the implants such as Guided Tissue Regeneration (GTR) often utilized in recovering
procedures that result in inflammation interfering with the bone regeneration.

Methods:
In the current study, a nano-hybrid GTR membrane is developed as a heliacal structure scaffold
with localized drug delivery function (Ibuprofen) as an anti-inflammatory agent. Polycaprolactone
(PCL) and a blend of Polyvinyl alcohol (PVA)/collagen (Col) (50/50) were electrospun by electrospinning.
Ibuprofen (Ibu) was loaded once in the PCL context and once in the hydrophilic portion
(PVA/Col).

Results:
The in vitro release behavior was investigated in each case. Chemical and physical properties
were studied for each item. Morphology investigation indicated a heliacal structure with the total average
diameter of 1266 nm consististing of porous pores with the average diameter of 256nm.

Conclusion:
The membranes indicated proper mechanical properties and appropriate biodegradation
rate as a potential GTR membrane. The controlled and sustained release of Ibu was obtained from both
PCL and PVA/COL loaded membranes. Kinetic model study indicated the following zero-order and
Higuchi models for the optimum case of PCL loaded and PVA/Col Ibu loaded scaffolds respectively.
Prader-Willi Syndrome - Clinical Genetics, Diagnosis and Treatment Approaches: An Update
Authors: Butler Merlin G. , Miller Jennifer L. , Forster Janice L. 
Published: July 2019

Background:Prader-Willi Syndrome (PWS) is a neurodevelopmental genomic imprinting disorder with lack of expression of genes inherited from the paternal chromosome 15q11-q13 region usually from paternal 15q11-q13 deletions (about 60%) or maternal uniparental disomy 15 or both 15s from the mother (about 35%). An imprinting center controls the expression of imprinted genes in the chromosome 15q11-q13 region. Key findings include infantile hypotonia, a poor suck, failure to thrive and hypogonadism/hypogenitalism. Short stature and small hands/feet due to growth and other hormone deficiencies, hyperphagia and marked obesity occur in early childhood, if uncontrolled. Cognitive and behavioral problems (tantrums, compulsions, compulsive skin picking) are common.Objective:Hyperphagia and obesity with related complications are major causes of morbidity and mortality in PWS. This report will describe an accurate diagnosis with determination of specific genetic subtypes, appropriate medical management and best practice treatment approaches.Methods and Results:An extensive literature review was undertaken related to genetics, clinical findings and laboratory testing, clinical and behavioral assessments and summary of updated health-related information addressing the importance of early PWS diagnosis and treatment. A searchable, bulleted and formatted list of topics is provided utilizing a Table of Contents approach for the clinical practitioner.Conclusions:Physicians and other health care providers can use this review with clinical, genetic and treatment summaries divided into sections pertinent in the context of clinical practice. Frequently asked questions by clinicians, families and other interested participants or providers will be addressed.
Pulmonary Pathology Among Patients with Type 2 Diabetes Mellitus: An Updated Systematic Review and Meta-analysis
Authors: Saini Minaxi , Kulandaivelan Sivachidambaram , Bansal Virender Kumar , Saini Vikram , Sharma Suresh , Kaur Jaspreet , Sondh Aparajita 
Published: July 2019

Background:Restrictive lung pathology was reported in the previous meta-analysis among patients with Type 2 Diabetes mellitus (T2DM) which is date back to 2010.Objective:To see the effect of T2DM on pulmonary functions through updated systematic review and meta-analysis.Data source:PubMed.Study eligibility criteria:English language case-control or cross-sectional studies, published between 1 January 2010 to 31 August 2018.Participants and intervention:We selected 22 articles that met our inclusion and exclusion criteria. Results reveal that among patients with T2DM all variables were reduced except %FEV1/FVC which shows statistically nonsignificant results with P=0.46. This confirms that T2DM patients have a restrictive type of lung pathology. Limitation: Only articles from the PubMed database were included.Study appraisal and synthesis methods:Methodological quality of the study was assessed using Newcastle-Ottawa Quality Assessment Scale. Meta-analysis was done using Review Manager 5.3 (RevMan 5.3) and meta-regression was conducted using R statistical software.Results:We selected 22 articles that met our inclusion and exclusion criteria. Results reveal that among patients with T2DM all variables were reduced except %FEV1/FVC which shows statistically nonsignificant results with P=0.46. This confirms that T2DM patients have a restrictive type of lung pathology. Limitation: Only articles from the PubMed database were included.Limitation:Only articles from PubMed database were included.Conclusion and implications of key findings:This review affirms the existing evidence of restrictive pathology among patients with T2DM. The future study could be conducted to see the effect of various rehabilitation protocols on pulmonary function among patients with T2DM.
Analysis of Germin-like Protein Genes (OsGLPs) Family in Rice Using Various In silico Approaches
Authors: Ilyas Muhammad , Irfan Muhammad , Mahmood Tariq , Hussain Hazrat , Latif-ur-Rehman  , Naeem Ijaz , Khaliq-ur-Rahman  
Published: July 2019

Background:
Germin-like Proteins (GLPs) play an important role in various stresses.
Rice contains 43 GLPs, among which many remain functionally unexplored. The computational
analysis will provide significant insight into their function.

Objective:
To find various structural properties, functional importance, phylogeny and expression
pattern of all OsGLPs using various bioinformatics tools.

Methods:
Physiochemical properties, sub-cellular localization, domain composition, Nglycosylation
and Phosphorylation sites, and 3D structural models of the OsGLPs were predicted
using various bioinformatics tools. Functional analysis was carried out with the Search Tool for
the Retrieval of Interacting Genes/Proteins (STRING) and Blast2GO servers. The expression
profile of the OsGLPs was predicted by retrieving the data for expression values from tissuespecific
and hormonal stressed array libraries of RiceXPro. Their phylogenetic relationship was
computed using Molecular and Evolutionary Genetic Analysis (MEGA6) tool.

Results:
Most of the OsGLPs are stable in the cellular environment with a prominent expression in
the extracellular region (57%) and plasma membrane (33%). Besides, 3 basic cupin domains, 7
more were reported, among which NTTNKVGSNVTLINV, FLLAALLALASWQAI, and
MASSSF were common to 99% of the sequences, related to bacterial pathogenicity, peroxidase
activity, and peptide signal activity, respectively. Structurally, OsGLPs are similar but functionally
they are diverse with novel enzymatic activities of oxalate decarboxylase, lyase, peroxidase, and
oxidoreductase. Expression analysis revealed prominent activities in the root, endosperm, and
leaves. OsGLPs were strongly expressed by abscisic acid, auxin, gibberellin, cytokinin, and
brassinosteroid. Phylogenetically they showed polyphyletic origin with a narrow genetic
background of 0.05%. OsGLPs of chromosome 3, 8, and 12 are functionally more important due to
their defensive role against various stresses through co-expression strategy.

Conclusion:
The analysis will help to utilize OsGLPs in future food programs.
Random Mutagenesis of Thermophilic Xylanase for Enhanced Stability and Efficiency Validated through Molecular Docking
Authors: Chauhan Shweta , Jaiswal Varun , Attri Chandrika , Seth Amit 
Published: July 2019

Background:
Xylanases of thermophilic origin are more robust and stable and hence
more suitable for industrial applications. The aim of the research was to develop a patent using a
robust mutant exhibiting enhanced xylanase activity. The strain (Bacillus aestuarii SC-2014) subjected
to mutagenesis is thermophilic in origin and hence it is envisioned that the enhancement of
its catalytic potential will enhance its industrial applicability.

Objective:
The main aim was to develop a stable and vigorous mutant having higher xylanase
activity and improved thermostability.

Method:
The bacterial strain isolated from the Tattapani hot springs of Himachal Pradesh (India)
was mutagenized by single separate exposure of Ethyl methane sulphonate (EMS) and N-methyl
N-nitro N-nitrosoguanidine (MNNG).

Results:
A mutant library was generated and extensive screening led to the identification of the
most potent mutant strain selected and designated as Bacillus sp. SC-2014 EMS200 (MTCC number
25046) which displayed not only enhanced xylanase activity and thermo stability but also appreciable
genetic stability. This strain displayed a 3-fold increase in enzyme activity and simultaneously,
a significant reduction in fermentation time from 72 h to 48 h was also observed. The xylanase
gene from wild and mutant strain was cloned, sequenced and subjected to molecular docking.
Two mutations H121D and S123T were present inside the binding pocket.

Conclusion:
Mutation H121D made the binding pocket more acidic and charged, thus enhancing
the xylanase activity for mutant protein. Mutations also resulted in charged amino acids (Y99K
and H121D) which were identified as a probable cause for enhancing the thermostability of mutant
protein.
Prominence of Oxidative Stress in the Management of Anti-tuberculosis Drugs Related Hepatotoxicity
Authors: John Preena , Kale Pravin P. 
Published: July 2019

:
Advanced medical services and treatments are available for treating Tuberculosis. Related
prevalence has increased in recent times. Unfortunately, the continuous consumption of related drugs is
also known for inducing hepatotoxicity which is a critical condition and cannot be overlooked. The
present review article has focused on the pathways causing these toxicities and also the role of enzyme
CYP2E1, hepatic glutathione, Nrf2-ARE signaling pathway, and Membrane Permeability Transition as
possible targets which may help in preventing the hepatotoxicity induced by the drugs used in the
treatment of tuberculosis.
Designing of an Epitope- Based Universal Peptide Vaccine against Highly Conserved Regions in RNA Dependent RNA Polymerase Protein of Human Marburg Virus: A Computational Assay
Authors: Mahmud S.M. Neaz , Rahman Mahbubur , Kar Antora , Jahan Nasreen , Khan Arif 
Published: July 2019

Introduction:
Marburg viruses are a group of negative-stranded RNA virus. It was first identified
in 1967 during a small outbreak. During that outbreak, the fatality rate increased highly and so many
people died by the Marburg virus. Later seven strains of Marburg virus were identified from those infected
humans. This virus causes Marburg Virus Disease (MVD) in human referred to as Marburg hemorrhagic
fever. Marburg virus is endemic only to Africa; however, there have been outbreaks in Europe and the
U.S.A. in recent times.

Objective:
However, the Marburg virus has a high fatality rate, so a preventive measure should be taken to
prevent infection. As there is no effective therapeutic agent available against these viruses, effective vaccine
design touching all strains would be a great step for human health.

Methods:
In our recent study, we used in silico analysis for designing a novel epitope-based vaccine against
all strains of Marburg virus. As it consists of several structural proteins and multiple sequence alignment
(MSA) of Glycoproteins, RNA-directed RNA polymerases, Nucleoproteins, Vp24 proteins, Vp30, Vp35, and
Vp40 proteins showed all strains of Marburg virus were conserved in RNA-directed RNA polymerase proteins.
Using that protein’s conserved region, T-cell and B-cell epitopes were determined.

Results:
Among the predicted epitope, only TIGNRAPYI was found to be highly immunogenic with 100%
conservancy among all strain of human Marburg virus. The analysis also showed both types I and II major
histocompatibility complex molecules interact with this epitope and found to be nonallergenic too.

Conclusion:
In vivo study of the proposed peptide is suggested for novel universal vaccine production that
might be an effective way to prevent human Marburg virus disease.
Expression of microRNAs (133b and 138) and Correlation with Echocardiographic Parameters in Patients with Alcoholic Cardiomyopathy
Authors: de Araújo Melo Leiliandry , da Silveira Maria Mariana Barros Melo , de Vasconcellos Piscoya Isabelle Cecília , Brasileiro Victor Arthur Eulálio , Farias Isabela Cristina Cordeiro , do Ó Kleyton Palmeira , Roncal Carlos Guilhermo Piscoya , Lima Raul Emídio , Filho Dário Celestino Sobral , Vasconcelos Luydson Richardson Silva 
Published: July 2019

Introduction:
Alcoholic Cardiomyopathy (ACM) is a disease with a difficult diagnosis.
The real mechanisms related to its pathophysiology are not fully understood.

Objective:
The aims of this study were to investigate whether miR-133b and miR-138 could be associated
with ACM.

Method:
Forty-four patients were included comprising 24 with ACM and 20 with cardiomyopathies
of different etiologies (control group). Real-time PCR was performed to verify the relative expression
among the studied groups. In the statistical analysis, the quantitative variables t-student Mann-
Whitney and correlation of Pearson tests were carried out, while the qualitative variable comprised
the chi-square test, with p<0.05 being considered statistically significant.

Results:
There was no association between clinical and sociodemographic characteristics of the
groups. The patients with ACM presented downregulation of miR-133b in comparison with control
patients (p=0.004). On the other hand, for the miR-138, there was no association when the ACM
group was compared with the control group. The presence of miR-133b among cases and controls
was not correlated with any of the echocardiographic parameters. However, the increase in the expression
of miR-138 was correlated with an increase in the ejection fraction (r=0.28, p=0.01) and the
diameter of the left atrium (r=0.23, p=0.04) in patients with ACM.

Conclusion:
The downregulation of miR-133b might be a marker for ACM and, in addition, miR-
138 could be used to correlate the increase in ejection fraction with and normalization of the diameter
of the left atrium diameter in patients with this disease.
Transient Left Ventricular Acute Failure after Cocaine Use
Authors: Cardinale Michaël , Esnault Pierre , Schmitt Johan , Meaudre Eric 
Published: July 2019

Background:
Cocaine is one of the most widely used illicit drugs, and it is the most
common cause of drug-related death. The association of cocaine use with acute heart failure is a rare
occurrence.

Case Report:
We report the case of a 31 years-old woman who presented Takotsubo cardiomyopathy
with severe cardiogenic shock after cocaine abuse. That required the use of high doses of positive
inotropic amines and mechanical ventilation. The evolution was quickly favorable after the cessation
of cocaine.

Discussion:
Takotsubo cardiomyopathy is often related to a stressful trigger, and several cases have
been described with the use of several psychostimulants. As such, it is not surprising that cocaine
use can be associated with the development of Takotsubo cardiomyopathy when it results in excess
release of catecholamines and excitation of adrenergic receptors.

Conclusion:
In critical care unit, Takotsubo cardiomyopathy is a rare complication of cocaine
abuse. This etiological diagnosis can be difficult especially in the absence of the concept of toxic intake
as it was initially the case for our patient, but the treatment does not differ from other causes of
cardiomyopathy and Cessation of cocaine use has been associated with improvement in cardiac
function.
Efectividad de hongos micorrizo-arbusculares nativos en suelos con diferentes usos agropecuarios
Authors: Martínez A Judith , Osorio V Nelson , Garrido P Jeyson 
Published: July 2019

Objetivo. Determinar la efectividad de hongos micorrizo-arbusculares (HMA) de suelos con diferentes usos agropecuarios, en el valle medio del río Sinú, Colombia. Materiales y métodos. Bajo condiciones de invernadero se realizó el experimento, donde se utilizó como planta indicadora Leucaena leucocephala, la cual se sembró en materos con suelo de un Andisol (horizonte B) usado como sustrato. Este sustrato, se inoculó separadamente con muestras de siete suelos (horizonte Ap), como fuente de HMA, provenientes del Centro de Investigación Turipaná de Agrosavia. Como controles se incluyeron inoculación con HMA Glomus fasciculatum y uno no-inoculado El efecto de la inoculación micorrizal se evaluó mediante el monitoreo del contenido de P foliar en las plantas de L. leucocephala, en función del tiempo hasta 64 días cuando se determinó la masa seca de su parte aérea y la colonización micorrizal. Resultados. El crecimiento de L. leucocephala y el contenido de P foliar fue significativamente superior cuando el suelo se inoculó con G. fasciculatum, en comparación a aquel observado con los demás tratamientos. De igual forma, la colonización micorrizal fue muy alta en las raíces de L. leucocephala que crecieron en el suelo inoculado con G. fasciculatum e inferior en los otros tratamientos. Conclusiones. Los resultados indican que los HMA nativos de suelos con diferentes usos, provenientes del C.I Turipaná de Agrosavia, exhibieron bajo potencial para desarrollar simbiosis micorrizal, lo que limitó el crecimiento y la concentración de P foliar en la planta hospedera.
Feasibility and Health Benefits of an Individualized Physical Activity Intervention in Women With Metastatic Breast Cancer: Intervention Study
Authors: Delrieu Lidia , Pialoux Vincent , Pérol Olivia , Morelle Magali , Martin Agnès , Friedenreich Christine , Febvey-Combes Olivia , Pérol David , Belladame Elodie , Clémençon Michel , Roitmann Eva , Dufresne Armelle , Bachelot Thomas , Heudel Pierre Etienne , Touillaud Marina , Trédan Olivier , Fervers Béatrice 
Published: July 2019

Background
There is limited knowledge regarding the potential benefits of physical activity in patients with metastatic breast cancer.

Objective
The Advanced stage Breast cancer and Lifestyle Exercise (ABLE) Trial aimed to assess the feasibility of a physical activity intervention in women with metastatic breast cancer and to explore the effects of physical activity on functional, psychological, and clinical parameters.

Methods
The ABLE Trial was a single-arm, 6-month intervention study with a home-based, unsupervised, and personalized walking program using an activity tracker. At baseline and 6 months, we assessed anthropometrics, functional fitness, physical activity level, sedentary behavior, quality of life, fatigue, and tumor progression. Paired proportions were compared using the McNemar test and changes of parameters during the intervention were analyzed using the Wilcoxon signed-rank test, the Mann-Whitney test, and Spearman rank correlations.

Results
Overall, 49 participants (mean age 55 years; recruitment rate 94%) were enrolled and 96% adhered to the exercise prescription (attrition rate 2%). Statistically significant improvements in the 6-minute walking distance test (+7%, P<.001) and isometric quadriceps strength (+22%, P<.001), as well as decreases in body mass index (-2.5%, P=.03) and hip circumference (-4.0%, P<.001) were observed at 6 months. Quality of life remained stable and a nonstatistically significant decrease (-16%, P=.07) in fatigue was observed.

Conclusions
The high recruitment and adherence rates suggest the willingness of patients with metastatic breast cancer to participate in a physical activity program. The beneficial outcomes regarding physical fitness and anthropometry of this unsupervised physical activity program may encourage these patients to maintain a physically active lifestyle. Future randomized controlled trials with larger sample sizes are warranted.

Trial Registration
ClinicalTrials.gov NCT03148886; https://clinicaltrials.gov/ct2/show/NCT03148886