bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison Between Environmental DNA Metabarcoding and Traditional Survey Method to Identify Community Composition and Assembly of Stream Fish
Authors: Liu Ziyu , Hu Cong , You Wenhui , Li Shuxin , Wu Yongsheng , Liang Yangyang , Chu Ling , Yan Yunzhi , Zhang Chen 
Published: November 2024

ABSTRACTEnvironmental DNA (eDNA) metabarcoding has been widely used in freshwater systems, contributing to the advancements in the monitoring of fish diversity and community species composition. Nevertheless, the accuracy and reliability of eDNA metabarcoding in assessing functional structures and revealing the mechanisms underlying fish community assembly remain unclear. In this study, we combined a traditional survey method (electrofishing) and eDNA metabarcoding to conduct fish stock monitoring in the upper reaches of the Huishui stream. We assessed taxonomic and functional structures, as well as community assembly mechanisms, during dry and wet seasons. The results revealed that, compared with electrofishing surveys, eDNA metabarcoding detected a greater number of species and higher functional richness in both seasons. Despite significant differences in fish taxonomic composition between the seasons, both eDNA and traditional methods indicated that environmental filtering dominated the process of fish community assembly in both dry and wet seasons. We showed that eDNA metabarcoding is comparable to the electrofishing method in monitoring the community composition of stream fish and can accurately and reliably determine fish community assembly mechanisms. Combining functional traits and eDNA is a robust approach for monitoring stream fish community compared to taxonomic uncertainty.
A global database of butterfly species native distributions
Authors: Daru Barnabas H. 
Published: November 2024

AbstractButterflies represent a diverse group of insects, playing key ecosystem roles such as pollination and their larval form engage in herbivory. Despite their importance, comprehensive global distribution data for butterfly species are lacking. This lack of comprehensive global data has hindered many large‐scale questions in ecology, evolutionary biology, and conservation at the regional and global scales. Here, I use an integrative workflow that combines occurrence records, alpha hull polygons, species' dispersal capacity, and natural habitat and environmental variables within a framework of species distribution models to generate species‐level native distributions for butterflies at a global scale in the contemporary period. The database releases native range maps for 10,372 extant species of butterflies at a spatial grain resolution of 5 arcmin (~10 km). This database has the potential to allow unprecedented large‐scale analyses in ecology, biogeography, and conservation of butterflies. The maps are available in the WGS84 coordinate reference system (EPSG:4326 code) and stored as vector polygons in the GEOPACKAGE format for maximum compression, allowing easy data manipulation using a standard computer. I additionally provide each species' spatial raster. All maps and R scripts are open access and available for download in Dryad and Zenodo, respectively, and are guided by FAIR (Findable, Accessible, Interoperable, and Reusable) data principles. By making these data available to the scientific community, I aim to advance the sharing of biological data to stimulate more comprehensive research in ecology, biogeography, and conservation of butterflies.
Characterizing Asymptotic Dependence between a Satellite Precipitation Product and Station Data in the Northern US Rocky Mountains via the Tail Dependence Regression Framework With a Gibbs Posterior Inference Approach
Authors: Russell Brook T. , Ding Yiren , Huang Whitney K. , Dyer Jamie L. 
Published: November 2024

ABSTRACTThe use of satellite precipitation products (SPP) allows for precipitation information to be collected nearly globally, but questions remain regarding their ability to reproduce extreme precipitation over mountainous terrain. In this work, we assess the ability of the precipitation estimation from remotely sensed information using artificial neural networks‐climate data record (PERSIANN‐CDR) to capture daily precipitation extremes by comparing PERSIANN‐CDR with corresponding station data in the summer at remote locations in the northern US Rocky Mountains of Wyoming, Idaho, and Montana. The assessment utilizes the regular variation framework from extreme value theory and consists of two parts: (1) evaluating the extent to which PERSIANN‐CDR can capture precipitation extremes through inference on an asymptotic dependence parameter, concluding that the level of asymptotic dependence is moderate throughout the region; (2) developing a tail dependence regression modeling framework and a Gibbs posterior approach for inference to investigate the degree to which elevation and topographic heterogeneity impact the level of asymptotic dependence, finding that the inclusion of a set of meteorological covariates, when combined with the PERSIANN‐CDR output, yields an increased level of asymptotic dependence with station data.
A System to Store Waste Heat as Liquid Hydrogen Assisted by Organic Rankine Cycle, Proton Exchange Membrane Electrolyzer, and Mixed Refrigerant Hydrogen Liquefaction Cycle
Authors: Nikzad Abolfazl , Mafi Mostafa , Faramarzi Saman 
Published: November 2024

ABSTRACTThis study proposes a system to store waste heat as liquid hydrogen using a proton exchange membrane electrolyzer (PEME) and a mixed refrigerant hydrogen liquefaction cycle. The novelty of this study lies in proposing a waste heat recovery system that stores electricity as liquid hydrogen, consuming less power due to the improved exergy efficiency of the components. The proposed system is analyzed to achieve better efficiency in terms of thermal and exergy efficiencies. Waste heat is used to generate power by an organic Rankin cycle (ORC), produced electricity is utilized in the PEME unit and compressors of liquefaction cycle to produce and liquefy hydrogen, respectively. Codes are written in EES software to simulate the system. Thermodynamic analysis is done in order to achieve better thermal efficiency for the proposed model. Membrane potential at different values of current density is calculated and compared with validate the simulated model. The exergy efficiency of the liquid hydrogen production process is 57%. The exergy efficiency, rate of power produced in ORC, and rate of hydrogen production by the electrolyzer increase significantly by increasing the isentropic efficiency of the turbine. At a temperature of 340 K for the evaporator, the thermal efficiency of ORC is obtained at 8.5%, which is approximately 3% higher compared with that of the previous similar process.
Optimization of Functional Gluten‐Free Cake Formulation Using Rice Flour, Coconut Flour, and Xanthan Gum via D‐Optimal Mixture Design
Authors: Taromsari Aidin , Ghiassi Tarzi Babak 
Published: November 2024

ABSTRACTProlamins in wheat, barley, and rye cause celiac disease (CD), non‐celiac gluten sensitivity (NCGS), and wheat allergies (WA). Although rice can be a suitable alternative, gluten‐free rice flour products are less technologically advanced. However, coconut flour and xanthan gum could enhance product quality, fiber content, and functional properties. This study employed a response surface D‐optimal mixture design to investigate the impact of substituting Sadri Hashemi rice flour (RF) (15.60%–31.19%) with low‐fat desiccated coconut flour (CF) (0%–15.59%) and xanthan gum (XG) (0%–0.31%) in 17 runs based on batter weight. Eleven responses were analyzed and exhibited significant and valid models (p < 0.0001), with satisfactory coefficient of determination (R2 > 0.85) values and non‐significant lack of fit (p > 0.1). Substitution at all levels significantly increased batter viscosity (R2 = 0.990) and specific weight (R2 = 0.995). Differences in cake crumb color (ΔE) decreased when RF was substituted with CF, while maximum ΔE elevation was observed with 0.31% XG due to synergistic effects with 5.09% CF (R2 = 0.974). Increasing the RF/XG ratio (0% CF) resulted in decreased water activity (aw), while increasing CF increased aw synergistically (R2 = 0.986). Moisture and texture hardness increased significantly with RF substitution up to 7.518% CF and 0.170% XG and 8.944% CF and 0.285% XG, respectively (R2 = 0.976 and 0.993). The volume index decreased with CF and XG (R2 = 0.989). Sensory acceptances were reduced by increasing XG while improved with optimal CF levels, predicted at 6.415% (R2 = 0.997), 6.784% (R2 = 0.999), 9.001% (R2 = 0.999), and 5.924% (R2 = 0.999) for color, taste, texture, and overall acceptance, respectively. Two‐sided prediction interval confirmation runs with 95% confidence (p ≤ 0.05) validated the models' accuracy and prediction of optimized combinations. The optimized gluten‐free cake formula (23.395% RF, 7.795% CF, 0% XG) achieved an 88.0% desirability score. Developing valid regression models facilitates a comprehensive investigation of cake quality and sensory properties, ensuring a viable gluten‐free product for individuals with gluten‐related disorders.
Systematic Exploration of the Association Between Vitamin A Intakes and Sarcopenia Prevalence in American Adults
Authors: Fang Meisi , Ma Jinghang , Bai Yongyu , Ying Yifan , Shen Xian , Feng Zhen 
Published: November 2024

ABSTRACTSarcopenia, characterized by an age‐related progressive loss of muscle mass and strength, presents significant health concerns. Recommending dietary nutrition emerges as a viable strategy to counteract muscle deterioration. Vitamin A, indispensable throughout the human life cycle and unattainable through endogenous synthesis, necessitates intake via diet. However, the direct correlation between sarcopenia prevalence and vitamin A intake remains unclear. This study systematically investigated the relationship between sarcopenia prevalence and vitamin A intake, including retinol and some carotenoids, across diverse races and genders utilizing multiple statistical analyses. Mixture analysis revealed significant positive correlations between total vitamin A intake and muscle mass among American adult males (Male: OR: 1.019, 95% CI: 1.010–1.027, p < 0.001). We also observed the gender‐specific results, with retinol playing a more significant role in enhancing muscle mass for males, while certain carotenoids were found to be more influential in females. Moreover, inflammation and oxidative stress mediated the relationship between vitamin A intake and sarcopenia prevalence in both genders. There may be a gender‐ and race‐specific relationship between dietary vitamin A intake and sarcopenia. Further prospective studies are imperative to elucidate the association between vitamin A intake and sarcopenia prevalence.
Clinical Management Update of Oral Leukoplakia: A Review From the American Head and Neck Society Cancer Prevention Service
Authors: Gates James C. , Abouyared Marianne , Shnayder Yelizaveta , Farwell D. Gregory , Day Andrew , Alawi Faizan , Moore Michael , Holcomb Andrew J. , Birkeland Andrew , Epstein Joel 
Published: November 2024

ABSTRACTBackgroundOral potentially malignant disorders (OPMDs) occur in up to 4%–5% of the population, of which oral leukoplakia (OL) is the most common subtype. Predicting high‐risk OL remains a challenge. Early diagnosis and effective treatment are thought to be of paramount importance to improve outcomes.MethodsWe searched PubMed and Clinicaltrials.gov data for updates in the clinical management of OL from 2015 to current.ResultsRecent publication of large cohorts of patients with OL aids in counseling patients regarding risk of malignant transformation. Management for OL includes surveillance, excision, and laser surgery, as well as local and systemic approaches to chemoprevention. Several new entities show promise regarding candidate biomarkers, chemoprevention agents, and diagnostic adjuncts, though all require further validation.ConclusionThis update serves to further inform clinical management of OL and provide impetus for future investigations.Trial RegistrationNCT00099021, NCT00951379, NCT05727761, NCT05727761
HPV E6/E7 mRNA screening alone can be used as a screening method for cervical cancer in premenopausal women in China: A retrospective study
Authors: Liu Ying , Yang Shirong , Ma Yingying , Gong Yingying , Du Beibei , Wang Yunfei , Yang Linqing 
Published: November 2024

AbstractBackgroundThis study aimed to assess the value of a HPV E6/E7 mRNA assay (Aptima® HPV [AHPV]) for primary cervical cancer screening combined with menopausal status.MethodsA total of 16 917 women underwent AHPV testing and had complete histopathological results at the Affiliated Hospital of Jining Medical University China between January 1, 2017 and March 31, 2022. We evaluated the performance of different screening strategies and combined strategies, as well as evaluations of different menopausal states.ResultsWhen identifying LSIL+ (includes low‐ and high‐grade squamous intraepithelial lesions and invasive cervical cancer [ICC]), the sensitivity (91.2%) and negative predictive value (NPV; 96.6%) were significantly higher for AHPV than for liquid‐based cytology assay (LBC; 33.2% and 84.7% for sensitivity and NPV, respectively). Furthermore, the co‐testing strategy (cytology combined with AHPV), when compared with AHPV, achieved a slightly higher sensitivity (93.6% vs. 91.2%, respectively, P < 0.001), a similar specificity (61.3% vs. 62.7%, respectively, P = 0.014), a similar positive predictive value (PPV; 37.5% vs. 37.8%, respectively, P = 0.709) and a similar NPV (97.5% vs. 96.6%, respectively, P = 0.001). Moreover, AHPV (when compared with menopausal women) achieved a higher sensitivity (93.5% vs. 77.7%, respectively, P < 0.001), a higher NPV (97.3% vs. 93.9%, respectively, P < 0.001), a similar PPV (37.8% vs. 37.0%, respectively, P = 0.618) and a slightly lower specificity (60.7% vs. 72.1%, respectively, P < 0.001) in premenopausal women. These results were similar when identifying HSIL+ (includes high‐grade squamous intraepithelial lesion and ICC).ConclusionThe present study suggests that initial screening with HPV E6/E7 mRNA testing rather than combined screening is a suitable candidate for cervical cancer screening in China (especially for premenopausal women) based on economic reasons.
A Lightweight Multimodal Xception Network for Glioma Grading Using MRI Images
Authors: Liang Yu , Li Dongjie , Ren Jiaxin , Gao Weida , Zhang Yao 
Published: November 2024

ABSTRACTGliomas are the most common type of primary brain tumors, classified into low‐grade gliomas (LGGs) and high‐grade gliomas (HGGs). There is a significant difference in survival rates between patients with different grades of gliomas, making imaging‐based grading a research hotspot. Current deep learning–based glioma grading algorithms face challenges, such as network complexity, low accuracy, and difficulty in large‐scale application. This paper proposes a multimodal, lightweight Xception grading network to address these issues. The network introduces convolutional block attention modules and employs dilated convolutions for spatial feature aggregation, reducing parameter count while maintaining the same receptive field. By integrating spatial and channel squeeze‐and‐excitation modules, it achieves more accurate feature learning, alongside improvements to the residual connection modules for critical information retention. Compared to existing methods, the proposed approach improves classification accuracy while maintaining a reduced parameter count. The network was trained and validated on 344 glioma cases (261 HGGs and 83 LGGs) and tested on 38 glioma cases (29 HGGs and 9 LGGs). Experimental results demonstrate that the network achieves an accuracy of 92.67% and an AUC of 0.9413 using a fully connected layer as the classifier. The features extracted using the improved Xception grading network achieved an accuracy of 93.42% when classified with KNN and RF classifiers. This study aims to provide diagnostic suggestions for clinical use through a simple, effective, and noninvasive multimodal medical imaging diagnostic method for LGG/HGG grading, thereby accelerating treatment decision‐making.
FMNL3 Promotes Migration and Invasion of Breast Cancer Cells via Inhibiting Rad23B‐Induced Ubiquitination of Twist1
Authors: Zhao Binggong , Ye Dong‐Man , Li Shujing , Zhang Yong , Zheng Yang , Kang Jie , Wang Luhong , Zhao Nannan , Ahmad Bashir , Sun Jing , Yu Tao , Wu Huijian 
Published: November 2024

ABSTRACTBreast cancer is a heterogeneous malignant tumor, and its high metastasis rate depends on the abnormal activation of cell dynamics. Formin‐like protein 3 (FMNL3) plays an important role in the formation of various cytoskeletons that participate in cell movement. The objective of this study was to explore the function of FMNL3 in breast cancer progression and endeavor to reveal the molecular mechanism of this phenomenon. We found that FMNL3 was abnormally highly expressed in aggressive breast cancer cells and tissues, and it significantly inhibited E‐cadherin expression. FMNL3 could specifically interact with Twist1 rather than other epithelial–mesenchymal transition transcription factors (EMT‐TFs). We also found that FMNL3 enhanced the repressive effect of Twist1 on CDH1 transcription in breast cancer cells. Further mechanism studies showed that FMNL3 suppressed the ubiquitin degradation of Twist1 by inhibiting the interaction between Twist1 and Rad23B, the ubiquitin transfer protein of Twist1. In vitro functional experiments, it was confirmed that FMNL3 promoted the migration and invasion of breast cancer cells by regulating Twist1. Furthermore, Twist1 could directly bind to the fmnl3 promoter to facilitate FMNL3 transcription. To conclude, this study indicated that FMNL3 acted as a pro‐metastasis factor in breast cancer by promoting Twist1 stability to suppress CDH1 transcription.
Development of a Japanese version of the Fresno test for family physicians to measure evidence‐based medicine competence
Authors: Kataoka Yoshihiro , Maeno Takami , Kimura Noriyuki , Ono Yuri , Suzuki Masatsune , Maeno Tetsuhiro 
Published: November 2024

AbstractBackgroundThe Fresno test is a useful instrument for measuring the evidence‐based medicine (EBM) competence of family physicians and others. This study aimed to develop a Japanese version of the Fresno test.MethodsFirst, the original version of the Fresno test was translated into Japanese following the standard instrument translation procedure. Subsequently, family medicine residents and certified physicians took the Japanese version of the Fresno test to assess its validity and reliability.ResultsTranslation of the Fresno test into Japanese was completed successfully. A total of 39 participants took the test to verify reliability and validity. Family medicine specialists scored higher than majors (129.7 ± 36.0 vs. 100.6 ± 45.1, p = 0.036), which indicated high construct validity. Intra‐class correlation coefficient and Kappa value of all questions were above 0.7, which suggested sufficient reliability. Item difficulty and floor/ceiling effect also fulfilled pre‐determined criteria.ConclusionsThis study successfully developed a Japanese version of the Fresno test. This test has sufficient reliability and validity and is expected to be useful as an instrument in EBM education for physicians in the future.
Real‐world practitioner perceptions of CARTITUDE‐4 results for patients with previously treated multiple myeloma
Authors: Balanean Alexandrina , Baird Samuel , Dulka Brooke , Jennings‐Zhang Luke , Bone Robert N. , Jeune‐Smith Yolaine , Feinberg Bruce , Baljevic Muhamed 
Published: November 2024

AbstractIntroductionInitially approved for the fifth‐line or later therapeutic setting, the chimeric antigen receptor (CAR) T‐cell regimen ciltacabtagene autoleucel (cilta‐cel) was recently approved for second‐line (2L) treatment in relapsed/refractory multiple myeloma (RRMM). Oncology practitioners use clinical trials to inform treatment, but real‐world impressions and impact on practice are lacking. We aimed to determine whether presenting CARTITUDE‐4 clinical trial data would impact real‐world preferences/perceptions around CAR T‐cell therapy.MethodsRecruiting from the Cardinal Health Oncology Provider Extended Network (OPEN), we surveyed hematologists/oncologists to investigate fourth‐line (4L) preferences in a hypothetical patient with triple‐class–refractory MM. We posed the same questions and answers before and after the trial presentation and compared pre‐/post‐preferences toward cilta‐cel and sequencing relative to bispecific antibodies (BsAbs). Using the same methodology as described above, we also performed a secondary analysis comparing pre‐/post‐perceptions on the use of CAR T‐cell therapy in earlier lines for patients with triple‐class–refractory MM.ResultsAmong 50 respondents, decision‐making factors before the trial presentation included CAR T‐cell center availability (58%), comorbidities (52%), and center locations (34%). Additionally, 48% of 46 respondents chose 4L cilta‐cel. Among 47, 40% wanted more real‐world/long‐term CAR T‐cell therapy outcomes in any line, 38% wanted more 2L data, and 34% favored 2L/third‐line (3L) use. After the presentation, the preference for cilta‐cel doubled from 48% to 88% (p < 0.001) among 50 respondents and rose from 34% to 55% (p = 0.001) for earlier‐line CAR T‐cell therapy among 49. Moreover, 55% of 49 respondents preferred CAR T‐cell therapy prior to BsAbs.DiscussionWe have shown that making oncology practitioners aware of trials precipitated decision‐making factors and led to notable, significant shifts in future intended practice patterns. Being aware of trial data enables practitioners to make more informed decisions, tailor therapies to individual patients, and ultimately improve outcomes.
Degenerateness, Mental Hygiene, and Spiritism: Debates in the Argentine Medical Press (1930–1946)
Authors: Parra Alejandro 
Published: November 2024

ABSTRACTThe disputes between spiritualists and physicians occurred in the context of hygienism and the degeneration theory, where spiritualists were considered agents requiring health care by alienists and psychiatrists. French psychiatry defended this interpretation to isolate and treat “spiritual delirium,” which came to have considerable importance in the debates between spiritism and psychiatry. Specifically, pathologization and psychologization became strategies to deal with the disruptive experience of mediumship and the sense of threat from spiritualism. Psychiatrists initiated anti‐spiritualist campaigns, inspiring responses from the spiritualist communities and their representatives, along with arguments to refute such diagnostic criteria. The debates between alienists and spiritists are an example of how, the rhetoric of spiritists, physicians, and some philosophers led to hostile positions regarding the designation of limits in the recognition of psychological and religious experiences.