bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wykrywanie mutacji w genie CALR oraz w genie ASXL1 u chorych na nadpłytkowość samoistną i samoistne włóknienie szpiku przy pomocy sekwencjonowania Sangera oraz analizy długości fragmentów DNA
Authors: Link-Lenczowska Dorota , Dryja Łukasz , Zapała Barbara , Krochmalczyk Dorota , Sacha Tomasz 
Published: February 2019

StreszczenieMutacje w eksonie 9 genuCALRoraz w eksonie 13 genuASXL1należą do markerów molekularnych o znaczeniu diagnostycznym i rokowniczym u chorych na nadpłytkowość samoistną (essential thrombocythemia– ET) oraz samoistne włóknienie szpiku (myelofibrosis– MF). Celem pracy było opracowanie i wdrożenie metod wykrywania mutacji w obu genach przy użyciu techniki sekwencjonowania Sangera oraz analizy długości fragmentów DNA. Przebadano 20 chorych na ET oraz 20 na MF. Sekwencjonowanie Sangera stosowano w wykrywaniu mutacji w obu genach, a analizę długości fragmentów DNA w wykrywaniu mutacji genuCALR. Typ 1 mutacji w genieCALRwykryto u 67% chorych na ET i u 86% chorych na MF, typ 2 mutacji potwierdzono u 15% ET i MF. Czułość diagnostyczna analizy długości fragmentów DNA wynosiła 3% obciążenia nieprawidłowym allelem, przy dolnej granicy detekcji 7-10% dla sekwencjonowania Sangera. Mutacje eksonu 13 genuASXL1wykryto u 25% chorych na MF (czułość 25%). Uzyskane wyniki wskazują na duże zalety praktyczne analizy długości fragmentów DNA jako techniki przesiewowej w diagnostyce ET oraz MF. Jej zastosowanie wraz z konwencjonalnym sekwencjonowaniem pozwala na wiarygodne wykrywanie i identyfikację aberracji genuCALR. Jednoczesna analiza mutacji somatycznych w genachCALRorazASXL1ułatwia diagnostykę różnicową chorych na MPN Ph- i służy stratyfikacji ryzyka w ich przebiegu.
Znaczenie badań molekularnych dla oceny ryzyka i rokowania u chorych na pierwotne włóknienie szpiku w oparciu o wskaźniki prognostyczne IPSS, DIPSS oraz MIPSS
Authors: Link-Lenczowska Dorota , Sacha Tomasz 
Published: February 2019

StreszczenieOcena ryzyka w przebiegu PMF opiera się o powszechnie uznawane skale prognostyczne. Wskaźniki IPSS oraz DIPSS bazują na parametrach klinicznych pacjenta, natomiast skala MIPSS uwzględnia dodatkowo mutacje somatyczne. Celem niniejszej pracy było dokonanie oceny ryzyka u chorych na PMF z wykorzystaniem skal IPSS, DIPSS i MIPSS oraz porównanie wyników stratyfikacji w oparciu o te wskaźniki. Według IPSS, DIPSS i MIPSS 10% pacjentów zostało zakwalifikowanych do grupy niskiego ryzyka, odpowiednio 40%, 60% i 35% do pośredniego – 1, a 25%, 20% i 55% do pośredniego – 2. Według IPSS i DIPSS 25% i 10% pacjentów zaszeregowano do grupy wysokiego ryzyka, według MIPSS żaden chory nie spełniał kryteriów kwalifikujących do tej grupy. Do wyższej kategorii ryzyka według MIPSS zakwalifikowano odpowiednio 25% oraz 35% pacjentów kwalifikowanych do niższej kategorii przy pomocy wskaźników IPSS oraz DIPSS (odpowiednio 15% i 23% pacjentów zmieniło kategorię ryzyka z pośredniego – 1 według IPSS i DIPSS na ryzyko pośrednie – 2 według MIPSS). Do niższej kategorii ryzyka według MIPSS przydzielono w sumie 45% pacjentów z kategorii większego ryzyka według skali IPSS i 20% według skali DIPSS. Zastosowanie skali prognostycznej MIPSS może zapewnić większą trafność w identyfikacji osób należących do wyższych kategorii ryzyka i doprowadzić do zmiany leczenia obejmującego także procedurę allo-HSCT.
Ruksolitynib w leczeniu pierwotnej mielofibrozy w fazie akceleracji i z małopłytkowością – opis przypadku
Authors: Cioch Maria 
Published: February 2019

StreszczeniePierwotna mielofibroza (primary myelofibrosis – PMF) jest Ph-negatywnym nowotworem mieloproliferacyjnym, charakteryzującym się włóknieniem szpiku, cytopeniami oraz objawami ogólnymi, które w istotny sposób wpływają na jakość i długość życia. Stosowanie ruksolitynibu, inhibitora kinaz JAK1 i JAK2 prowadzi do zmniejszenia wymiarów śledziony, a także nasilenia objawów ogólnych, co poprawia jakość życia. Tego typu terapia rekomendowana jest u chorych z objawową mielofibrozą z grupy ryzyka pośredniego 2 i wysokiego. Opublikowano dotąd niewiele doniesień dotyczących zastosowania ruksolitynibu w zaawansowanych fazach PMF: akceleracji i zaostrzenia blastycznego. Ważnym klinicznie problemem jest także prowadzenie chorych ze współistniejącą małopłytkowością.W pracy przedstawiono chorą na PMF, u której ruksolitynib z dobrym skutkiem stosowany jest od 4 lat w fazie akceleracji, przy współistniejącej małopłytkowości. Rozpoznanie PMF postawiono w 2008 r. W ciągu piewszych 5 lat w leczeniu stosowano hydroksykarbamid, talidomid, prednizon i radioterapię śledziony. W roku 2013 rozpoznano akcelerację choroby, charakteryzującą się obecnością 10% blastów w szpiku, splenomegalią dużego stopnia, małopłytkowością oraz nasileniem objawów ogólnych. Leczenie ruksolitynibem rozpoczęto w grudniu 2013 r. Dawka leku, zależnie od liczby płytek krwi, wahała sie od 5 mg raz dziennie do 20 mg 2 razy dziennie. Terapia ruksolitynibem jest kontynuowana do dzisiaj, tj. ponad 4 lata. Jej efektem jest redukcja wymiarów śledziony, ustąpienie objawów ogólnych, poprawa jakości życia oraz stabilizacja odsetka komórek blastycznych w szpiku. Prezentowany przypadek jest przykładem na skuteczność ruksolitynibu w fazie zaawansowanej PMF dzięki indywidualizacji dawkowania leku.
Zespół hemofagocytowy indukowany terapią hormonalną – studium przypadku klinicznego
Authors: Szymczyk Agnieszka , Klimek Monika , Podhorecka Monika , Kowal Małgorzata , Tomczak Waldemar , Kozińska Justyna , Suszek Dorota , Majdan Maria , Hus Marek 
Published: February 2019

StreszczenieZespół hemofagocytowy (ang. hemophagocytic lymphohistiocytosis – HLH) charakteryzuje się nieprawidłową aktywacją układu immunologicznego, u podłoża której leżą zmiany genetyczne lub nabyte zaburzenia cytotoksyczności limfocytów T i NK. Objawy kliniczne są niespecyficzne i różnorodne, a postawienie rozpoznania, pomimo dostępności badań dodatkowych, jest niezwykle trudne. W artykule zaprezentowano przypadek kliniczny chorej, u której pierwotnie w obrazie klinicznym dominowała nawracająca gorączka oraz zmiany skórne sugerujące rozpoznanie rumienia guzowatego. Pomimo rozpoczęcia steroidoterapii oraz stosowania empirycznej antybiotykoterapii stan pacjentki nie ulegał poprawie. Do objawów klinicznych dołączyły się splenomegalia oraz zaburzenia w badaniach dodatkowych: trójukładowa cytopenia, hiperferrytynemia, hipertriglicerydemia, hipofibrynogenemia. Ponadto w obrazie histopatologicznym szpiku kostnego stwierdzono obecność hemofagocytów. Na podstawie obrazu klinicznego oraz badań dodatkowych postawiono rozpoznanie HLH. W terapii zastosowano chemioterapię zgodnie z protokołem HLH-2004, uzyskując całkowitą remisję.
Assessment of Environmental Factors on Secondary Metabolites and Toxicological Effects of Datura metel Leaves Extracts
Authors: Raheem Modinah A.O. Abdul , Sulaiman Faoziyat A. , Abdulrahim Halimat A. , Ahmed Olatunde , Yusuf Kamil O. , Mukadam Abdulrahman A. , Ganiyu Latifat O. , Kamilu Lateef A. , Oladepo Khadijat B. , Alimi Grace O. , Afolayan Daniel , Odeniran Taiwo 
Published: February 2019

Abstract
This study evaluated the chemical composition, physicochemical properties, toxicological and environmental potentials of the Datura metel (D. metel) leaves extracts. Fresh leaves of D. metel were harvested at three different times of the day (morning, afternoon and evening), extraction done in succession using n-hexane and ethanol solvents respectively. Preliminary phytochemical screening, physicochemical analysis for specific gravity, pH, colour, total ash and moisture contents of the dried extracts were determined. The characterisation of the bioactive compounds using Gas Chromatography-Mass Spectrometry, showed the presence of different compounds at the three different times of the day, out of which phytol and 9,12,15-octadecatrienoic acid were present at all times. These results confirmed that some bioactive compounds present in this plant are dependent on environmental factor , time of harvest and the choice of solvent used while the presence of others are independent on these factors. Each extract was administered orally to Wistar rats for two weeks. There were significant difference (p < 0.05) in activities of AST (serum and heart), ALT (liver and serum) and ALP (Serum, liver and kidney), as well as the concentrations of albumin and protein in the liver and serum and urea in the serum of experimental rats given n-hexane and ethanolic extracts of D. metel. Generally it also suggest that the leaf extracts collected at three different times of the day had some significant toxicological effect, thus may not be totally safe for consumption at the dosage indicated since tissues membranes integrity of the Wistar rats were not potentially preserved.
Animal occurrence in fragmented forest habitats – important factors at the patch and landscape scale
Authors: Banul Rafał , Kosewska Agnieszka , Borkowski Jakub 
Published: February 2019

Abstract
To date, in research, the main determinants influencing animal assemblages in fragmented forests have been considered to be forest island area and degree of isolation. Such a simplistic approach may have detrimental effects on the obtained results and conclusions, since there are a large number of other factors determining animal persistence in forest islands. In order to identify these factors and evaluate their importance, we reviewed the scientific literature on the topic. In addition to the island area, also patch shape, edge effects and local plant community structure are crucial factors affecting animal assemblages at the forest island scale. At the landscape scale, the total number of forest islands and their combined area, matrix permeability, occurrence of wide ecological corridors as well as isolated trees and woodlands appear to be the most significant factors.
Our review further indicates that many of these elements also tend to interact. For instance, edge effects may reduce the area of suitable habitat in a forest patch. Furthermore, some fragmentation effects may be masked by species traits e.g. mobility, food preferences or habitat specialisation. The landscape context also plays a crucial role in animal persistence in fragmented forests. We thus conclude that there is a strong need to investigate the above-mentioned components of habitat fragmentation at the local and landscape scale using appropriate bio-indicators.
Taxonomic revision of bears from the locality Šandalja I (Croatia) and its biostratigraphic consequences
Authors: Wagner Jan , Jiangzuo Qigao , Lenardić Jadranka Mauch , Liu Jinyi 
Published: February 2019

Abstract
Šandalja I palaeocave (“fossil” cave), situated in the southern part of the Istrian peninsula (Croatia), is a well-known locality due to the abundant fossil remains extracted from the bone breccia. It is broadly accepted that the fauna is Middle Villafranchian in age. Since the beginning, the taxonomic status of bear remains has caused controversy and, as a consequence, different authors listed between one to three bear species from this site. Detailed revision of dental material confirmed the presence of two bear species – Ursus thibetanus and U. deningeri. On the other hand, the previously reported presence of U. etruscus was refuted. Subsequently, based on the presence of these two bear taxa, the age of the whole faunal assemblage was re-evaluated and determined as Middle Pleistocene (Toringian).
Internal Return Migration in Rural of Vietnam: Reasons and Consequences
Authors: Thanh Ngo Trung , Lebailly Philippe , Dien Nguyen Thi 
Published: February 2019

Abstract
Many researchers have tried to explain the motivation behind out and return migration. However, few bodies of literature focus on selection of destinations of out migration, motives to return according to marriage status of migrants before the return and gender perspective of employments on the return. By surveying 68 returnees and applying participatory rural appraisal, this study shows that the personal and household characteristics of returnees before the migration create an effect on destination selections On the return, both single and married migrants are motivated by filial obligations to their parents. Single migrants’ motive associates to the potential failures that can be a burden on their livings after getting married. Married migrants’ motive to return results from living away from their children. This study also demonstrates that women play an important role in the development of agriculture. Additionaly, agriculture acts as a buffer to the negative impacts of return migration.
Dual Vocational Students’ Competency: A Second Order Confirmatory Factor Analysis of Occupational Competency in Enterprise
Authors: Boonsri Somchat , Pupat Phadungchai , Suwanjan Peerawut 
Published: February 2019

Abstract
Due to rapid change in technology, enterprises are facing a shortage of competent human resources that are suitable for their needs. The Dual Vocational Training Program, which emphasizes authentic training for enterprises, is able to develop human resources from vocational education so that their competencies are up to date competencies, to keep up with technological changes. The purpose of this study was to conduct a second order confirmatory factor analysis of occupational competencies in enterprises. The samples of this study were 804 students of the Vocational Education Certificate Program, in the field of Mechanical Technology. They were selected by using Multi-Stage Random Sampling. The instrument used was a 5 level rating scale questionnaire. The results of the study indicated that the Model of Occupational Competency in Enterprise of Dual Vocational Training Students is valid. The structural equation statistical values were: Chi-square = 896.0707, df = 514, p = .000, χ2/df = 1.47 (less than 2.0), GFI = .945, AGFI = .920, and RMSEA = .030.
Study of selected physical, chemical and biological properties of selected materials intended for contact with human body
Authors: Mrówka Maciej , Machoczek Tomasz , Jureczko Paweł , Szymiczek Małgorzata , Skonieczna Magdalena , Marcoll Łukasz 
Published: February 2019

Abstract
The purpose of the conducted study was to analyse new materials intended for contact with the human body in view of their physical, chemical and biological properties. The authors have put to test six commercially available materials, four out of which were composite polyamide 12-based materials, while two were polyurethanes. The examined materials were assessed in terms of the surface. Subsequently, their hardness and biocompatibility were tested. The authors devoted major attention to the tests of absorption and emissivity of water, the pH = 7.4 PBS buffer solution and pH = 4.3 artificial sweat in temperatures of 21°C and 37°C. The results of the tests have confirmed the non-toxicity of all the tested materials and allowed to provide their characteristics in terms of their surface, hardness, as well as absorption and emissivity of various body fluids. Both polyamide 12 and the tested polyurethanes are classified as thermoplastics that may be used in additive technology.
The analysis of distribution of the reaction mixture in ammonia oxidation reactor
Authors: Moszowski Bartosz , Wajman Tomasz , Sobczak Krzysztof , Inger Marek , Wilk Marcin 
Published: February 2019

Abstract
This article describes the influence of various design modifications of the ammonia oxidation reactor operating in nitric acid plant TKIV in Kędzierzyn-Koźle on flow distribution of an air-ammonia mixture. The CFD (Computational Fluid Dynamics) simulations of turbulent flow were carried out with SST k-ω turbulence model to close the system of RANS (Reynolds Averaged Navier-Stokes) equations. The simulation results show that the properly selected perforated plate screen and the conical diffuser ensure uniform flow of gas on the ammonia oxidation catalysts and on the catalysts for nitrous oxide decomposition. It was proved experimentally achieving uniform temperature of nitrous gases in different locations under the catalytic gauzes and high efficiency of ammonia oxidation and nitrous oxide decomposition
Terrorism and Neoterrorism. The Romanian Approach
Authors: Grozav Cosmin-Marius 
Published: February 2019

Abstract
The development of this true scourge in the whole world demands a more accurate knowledge and documentation on the emergence, development, operation, motivation that underlies terrorist actions, as well as a knowledge of the funding of these terrorist groups. Considering the fact that this scourge has existed since antiquity and the Middle Ages, so it has a true “history”, developed gradually in the 1700s, it has experienced a real “explosion” since 1968, reaching the peak in 2001, it is necessary that those entitled to know in depth this “phenomenon” and if they can not eradicate it, at least to unite their efforts at an international level and limit it as much as possible. At global level, there are sufficient determinants, but also reasons for this phenomenon to have a continuous development, and we are taking in consideration the continuous development of the armaments industry and the misguided policies of some governments.
Research Regarding The Quality of Sleep among Non-Sportive Students Compared with Professional Athlets
Authors: Sopa Ioan Sabin , Pomohaci Marcel 
Published: February 2019

Abstract
Quality of sleep developed to be a much disputed subject in everyday life performance of men. Our study focuses on comparing two samples one of non-sportive (formed by 42 students’ age 19 ± 1.22 years) and one of professional athletes (formed by 45 sportive age between 18 ± 2.01 years). The main method used for analyzing the quality of sleep between the two samples of the research was the Athlete Sleep Screening Questionnaire (ASSQ) that is a tool that analyses sleep behaviors, identify disorder in sleeping routine and determine the frequency of difficulties with sleep before and after events, competitions or intense training programs.
The results of the study showed a statistically significant differences between the two samples in the number of hours slept at night (the experiment sample having 8 to 9 hours of sleep compared with the control sample that have 6 to 7 hours); also we found differences between the time needed to fall asleep (less than 15 min at the experiment group compared with 31-60 min at the control group); less problems at sleeping in the experiment group compared with the control group and that the athletes from the experiment group are waking earlier and are a morning kind of persons compared with the control group that are more active at evening; also found differences at the caffeine doses consumed and usage of electronic devices before sleep time (the experiment have less cases compared with the control group). So the general conclusion of the study was that the level of satisfaction regarding the quality of sleep is higher at the experiment sample compared with the control sample.
Potential of osteopontin in the management of epithelial ovarian cancer
Authors: Cerne Katarina , Hadzialjevic Benjamin , Skof Erik , Verdenik Ivan , Kobal Borut 
Published: February 2019

Abstract

Background
Osteopontin (sOPN) is a promising blood tumour marker for detecting epithelial ovarian cancer (EOC). However, other clinical uses of sOPN as a tumour marker in EOC are still lacking. Since sOPN concentrations in serum are not associated with those in ascites, we compared clinical value of sOPN concentrations in the two body fluids.

Patients and methods
The study included 31 women with advanced EOC and 34 women with benign gynaecological pathology. In the EOC group, serum for sOPN analysis was obtained preoperatively, after primary debulking surgery and after chemotherapy. In the control group, serum was obtained before and after surgery. Ascites and peritoneal fluid were obtained during surgery. sOPN concentrations were determined by flow cytometry bead-based assay.

Results
The sensitivity and specificity of sOPN in detecting EOC was 91.2% and 90.3% (cut-off = 47.4 ng/ml) in serum, and 96.8% and 100% (cut-off = 529.5 ng/ml) in ascites. Kaplan-Meier analysis showed a significant association between higher serum sOPN concentration and overall survival (p = 0.018) or progression free survival (p = 0.008). Higher ascites sOPN concentrations were associated with suboptimally debulked tumour and unresectable disease. Higher serum sOPN concentrations were associated with refractory disease or incomplete response to platinum-based chemotherapy.

Conclusions
The study showed that ascites sOPN level mirrors present disease and is superior to serum level for diagnostic purposes and surgical planning, although the end result of treatment is the response of the whole body in fighting the disease. The preoperative sOPN concentration in serum thus better reflects disease outcome.
Romanian version of SDM-Q-9 validation in Internal Medicine and Cardiology setting: a multicentric cross-sectional study
Authors: Baicus Cristian , Balanescu Paul , Gurghean Adriana , Badea Camelia Georgeta , Padureanu Vlad , Rezus Ciprian , Mitu Florin , Jurcut Ruxandra , Balanescu Andra Rodica , Daha Ioana , Balanescu Eugenia , Bojinca Mihai , Pinte Larisa , Constantin Alexandru Marian , Dima Nicoleta , Floria Mariana , Leon-Constantin Maria Magdalena , Roca Mihai , Mitu Magda , Chiriac Silvia , Badescu Codruta Minerva , Ionescu Simona Daniela , Mitrea Elena , Rosu Gabriel , Ionescu Georgeta Daniela , Visinescu Ana Maria , Mihailescu Gabriela , Lapadatu Rozalina , Oprisan Emilia , Zeh Stefan , Scholl Isabelle , Härter Martin 
Published: February 2019

Abstract

Background
. Shared decision making (SDM) is becoming more and more important for the patient-physician interaction. There has not been a study in Romania evaluating patients’ point of view in the SDM process yet. Therefore, the present study aims to evaluate the psychometric parameters of the translated Romanian version of SDM-Q-9.

Material and methods
. A multicentric cross-sectional study was performed comprising eight recruitment centers. The sample consisted of in- and outpatients who referred to Hospital Units for treatment for atrial fibrillation or collagen diseases. Furthermore, patients who were members of Autoimmune Disease Patient Society were able to participate via an online survey. All participants completed the Romanian translated SDM-Q-9.

Results
. Altogether, 665 questionnaires were filled in within the hospital setting (n = 324; 48.7%) and online (n = 341; 51.3%). The Romanian version had good internal consistency (Cronbach α coefficient of 0.96.) Corrected item correlations were good ranging from 0.64 to 0.89 with low corrected item correlations for item 1 and item 7. PCA found a one-factorial solution (similar with previous reports) but the first item had the lowest loading.

Conclusion
. SDM-Q-9 is a useful tool for evaluation and improvement in health care that was validated in Romania and can be used in clinical setting in this country.