bluuebunny/crossref_metadata_2025_split huggingface dataset:
103 Puberty induction protocol, but not supplement amount, overcomes the negative impacts of reduced frequency of supplementation on reproduction of beef heifers
Authors: Ranches Juliana , Vedovatto Marcelo , Palmer Elizabeth , Miranda Miguel , Arthington John , Vendramini João , Moriel Philipe 
Published: July 2019

Abstract
This study evaluated the performance of Bos indicus-influenced beef heifers submitted to a puberty induction protocol and offered two supplementation amounts and delivery frequencies. Sixty-four Brangus crossbred heifers were stratified by BW and age (244 ± 4 kg; 311 ± 18 d) and assigned into 1 of 16 bahiagrass pastures on d 0. Treatments were randomly assigned to pastures in a 2 × 2 factorial design (4 pastures/treatment) and consisted of soyhulls-based supplementation at 1.25% or 1.75% of BW (DM basis) offered daily or 3 times weekly for 168 d. The puberty induction protocol consisted of CIDR insert on d 56 and removal on d 70, 5-mL PGF2a injection on d 86, 2-mL GnRH injection and timed-AI on d 89. Heifers were exposed to Angus bulls from d 89 to 167 (1 bull/pasture). Pregnancy diagnosis was assessed on d 212. Frequency × rate effects were not detected (P ≥ 0.19) for any variable. Reducing the supplementation frequency from daily to 3X weekly tended (P = 0.07) to decrease overall ADG and puberty attainment on d 56 (53 vs. 41 ± 6%), but not puberty attainment on d 89 (P = 0.45; 84 vs. 78 ± 6%) and pregnancy percentage on d 212 (P = 0.97; 69 vs. 69 ± 7%). Increasing the supplement amount from 1.25% to 1.75% of BW increased (P = 0.04) overall ADG, tended (P = 0.09) to hasten puberty attainment on d 56 (40 vs. 54 ± 6%) and d 89 (74 vs. 88 ± 6%), and enhanced (P = 0.03) pregnancy percentage on d 212 (53 vs. 80 ± 7%). Therefore, the inclusion of a puberty induction protocol, but not increasing the supplement amount, enabled a reduced frequency of supplementation (3 vs. 7 times weekly) without detrimental impacts on percentage of pregnant yearling beef heifers.
113 Influence of cattle stress response to restraint method on artificial insemination pregnancy rates
Authors: Carrell Russell C , Smith William B , Kinman Lea A , Mercadante Vitor R G , Roper David 
Published: July 2019

Abstract
Activation of physiological stress responses have been associated with decreased pregnancy rates in beef cattle. Therefore, alternative cattle restraint methods have been developed to reduce this negative impact. However, little evidence exists comparing pregnancy outcomes of differing methods. Thus our objective was to determine if a difference was present in pregnancy rate to fixed-time artificial insemination in Bos taurus beef females when using a breeding box (b) versus a squeeze chute (c). Cows and heifers from two separate locations (Virginia Tech University, VT; and R.A. Brown Ranch, RAB) were exposed to FTAI protocols at location of origin while restrained in a squeeze chute (n = 169) or a breeding box (n = 162). Females were exposed to the 7 day Co-Sync + CIDR protocol pre-breeding. At day of breeding, females were randomly assigned to treatment. Immediately following breeding, blood was collected via coccygeal venipuncture for assessment of plasma cortisol concentration using an automated ELISA assay (Immulite 2000 XPi, Siemens). Chute and Exit Score (1–5) were assessed at the time of breeding by a trained technician at each location. Pregnancy was verified thirty days post-breeding via rectal ultrasonography. Pregnancy rate (PROC FREQ) and cortisol (PROC GLIMMIX) were analyzed using SAS version 9.4. Also, a Linear Regression Model (SAS version 9.4) was used to determine relationships between chute score, exit score, and cortisol. It was found that there was no difference in pregnancy rate between the breeding box and the squeeze chute (61.73% vs 64.71%, P = 0.31, respectively). Cortisol showed no difference between b and c (59.44±10.17 vs 58.61±10.16, P = 0.68, respectively). There was no linear relationship between cortisol, exit score, and chute score (P = 0.14). It can be concluded that stress responses are similar between the breeding box and squeeze chute. Therefore females will have similar pregnancy rates when exposed to FTAI in either restraint mechanism.
154 Evaluation of repeated exposure to lipopolysaccharide on vaginal temperature and complete blood count in two-year-old Brahman heifers
Authors: O’Daniel Sydney E , Riley David G , Neuendorff Don A , Dailey Jeffery W , Carroll Jeffery A , Randel Ronald D , Welsh Thomas H 
Published: July 2019

Abstract
The effects of escalating lipopolysaccharide (LPS) doses on vaginal temperature (VT) and complete blood count (CBC) were evaluated to develop a model of low-level inflammatory response in cattle. Brahman heifers (2-yr-old; 326 kg BW) paired by birthdate, sire, and weaning temperament score were fitted with self-contained, indwelling vaginal temperature probes 7 d before initiation of treatment (d 0) and randomly assigned to Control (n = 6) or LPS (n = 6) treatment. Heifers were maintained as a group with free choice access to Coastal bermudagrass hay and fed 3.6 kg of a 3:1 corn:corn gluten grain mix per head per day. Heifers were weighed, BCS recorded, and blood samples were collected weekly by jugular venapuncture before delivery of treatments. Treated heifers received LPS (from Escherichia coli) via subcutaneous injection in the neck (d 0: 0.25 μg / kg BW; d7: 0.50 μg /kg BW; d 14: 0.75 μg /kg BW) and Control heifers received saline. Sickness behavior score (SBS) was monitored at 30-min intervals for 12 hr after LPS. Treatment was the fixed effect of interest. Random effects included sire and day repeated with heifer as the subject. No dose-treatment interactions were detected. The SBS did not change after subcutaneous LPS or saline (P > 0.1). Neutrophil and lymphocyte numbers 7 d after each injection were not affected by LPS (P > 0.1). A febrile response began within 1 hr and persisted up to 14 hr after LPS injections. The febrile effect of the 0.25 μg LPS dose was less pronounced (P < 0.05) than that of the 0.50 μg and 0.75 μg LPS doses which were similar to one another. Lipopolysaccharide treatment increased maximum VT (P < 0.0001), hastened time to attain maximum VT (P = 0.0429), and increased the change in VT (P < 0.0001). Weekly subcutaneous injection of LPS facilitates the study of the effect of sub-clinical illness in beef cattle.
150 Administration of prostaglandin F2α 24 h prior to CIDR removal impacts reproductive performance of suckled beef cows assigned to the 7-d CO-Synch + CIDR protocol
Authors: Fontes Pedro , Cooke Reinaldo F , Oosthuizen Nicola , Timlin Claire L , Dias Nicholas , Currin John F , Clark Sherrie , Lamb Graham Cliff , Pohler Ky G , Mercadante Vitor R G 
Published: July 2019

Abstract
Angus-influenced cows received 100 µg of GnRH and a CIDR device containing 1.38 g of progesterone (P4) on d 0, and were randomly assigned to 25-mg injection of PGF2α on d 6 (PG6; n = 147) or d 7 (PG7; n = 162). The CIDR device was removed on d 7, and cows received 100 µg of GnRH and artificial insemination (AI) on d 10. An estrus detection patch was attached to the tailhed of each cow on d 7, and estrus expression assessed at AI (> 50% coating removal). Blood samples were collected on d 0 to determine the presence of a functional corpus luteum via plasma P4 (CL, P4 ≥ 1.0 ng/mL; NOCL P4< 1.0 ng/ mL). A subset of cows (70 cows/treatment) were assigned to ultrasonography at AI to verify dominant follicle diameter (DFD). Blood samples for plasma P4 analysis were collected (42 cows/treatment) on d 6, and on d 7 at CIDR removal and 1 h later. A similar proportion of PG6 and PG7 cows were classified as CL (P = 0.38). Plasma P4 at CIDR removal and 1 h later were less in PG6 vs. PG7 cows classified as CL (1.81 vs. 3.99 and 1.06 vs. 2.89 ng/mL), and did not differ (P ≥ 0.43) within NOCL. Estrus expression was greater in PG6 vs. PG7 cows classified as CL (72.8 vs. 48.3%), but similar (P = 0.96) within NOCL. Pregnancy rates did not differ between treatments (P = 0.53), despite a numerical difference (P = 0.15) between PG6 vs. PG7 cows classified as CL (65.5 vs. 55.0%). No other treatment effects were detected (P ≥ 0.20). Hence, PGF2α administration 24 h prior to CIDR removal appears to benefit reproductive performance of beef cows with a functional CL at the beginning of the 7-d CO-Synch + CIDR protocol
151 Timing of concentrate supplementation during late gestation impacts calf pre-weaning growth, but not reproductive performance of Bos indicus-influenced cows
Authors: Palmer Elizabeth , Vedovatto Marcelo , Ranches Juliana , Miranda Miguel , Gouvêa Vinícius , Arthington John , Vendramini João , Moriel Philipe 
Published: July 2019

Abstract
This study evaluated the effects of timing of dried distillers grains (DDG) supplementation during late-gestation on performance of Bos indicus-influenced cow-calf pairs. At about 84 d before calving (d 0), 84 mature Brangus cows were stratified by BW and BCS (422 ±42 kg; BCS = 5.0 ± 0.14) and assigned randomly to bahiagrass pastures (14 cows/pasture). Treatments were assigned randomly to pastures (2 pastures/treatment) and consisted of no prepartum supplementation (CON), 1 kg/d of DDG from d 0 to 84 (LATE84; 84 kg of DM/cow); or 2 kg/d of DDG from d 0 to 42 (LATE42; 84 kg of DM/cow). The respective weekly supplement amount was divided and offered into 3 feeding events (Monday, Wednesday, and Friday). Cow BCS were assessed on d 0, 45, 84 (calving), 140 and 224 (start and end of breeding season, respectively). Calf BW was collected at birth and weaning (d 346). Growth and reproductive data were analyzed using SAS MIXED and GLIMMIX procedures, respectively. Cow BCS from d 45 to 224 did not differ between LATE84 and LATE42 cows (P ≥ 0.20) but was always the least (P ≤ 0.05) for CON cows. Calving date, calf birth BW, and percentage of pregnant cows on d 283 did not differ (P ≥ 0.19) among treatments. Calf pre-weaning ADG and BW at weaning did not differ (P ≥ 0.58) between LATE42 and CON calves, but LATE84 calves tended to have the greatest pre-weaning ADG (P = 0.10; 0.88, 0.83 and 0.82 ± 0.02 kg/d, respectively) and BW at weaning (P = 0.10; 263, 254 and 252 ± 4.4 kg, respectively). Therefore, timing of prepartum DDG supplementation did not impact cow reproductive performance. However, calf pre-weaning growth tended to be enhanced when cows received longer vs. shorter or no periods of prepartum DDG supplementation
121 Non-invasive monitoring of hot-iron brands on growing cattle
Authors: Tolleson Douglas R , Campbell Erika , Garza Nick , Moen Robert 
Published: July 2019

Abstract
Hot-iron branding is a traditional form of permanent cattle identification that produces a scar. There is a need for science-based determination of cattle brand age through non-invasive monitoring of the healing process. Near infrared reflectance spectroscopy (NIRS) has been used by medical forensic scientists to obtain such information. Healing of cutaneous injury involves inflammatory (1 to 3 d), proliferative (4 to 21 d), and remodeling (22 to 365 d) phases. Collagen changes as burn scars mature, i.e. there is an increase in the Type I/ Type III collagen ratio compared to normal skin. The altered ratio is evident in a transformation of collagen from a basketweave arrangement to small parallel bundles. During the remodeling phase, due largely to the Type I/III collagen ratio, scar tissue becomes visibly different than un-injured skin. Previous research has examined the differences in hot-iron brands applied to nursing (~30 d old) Bos taurus cross calves at 0, 33, and 153 d post-branding. Our objective was to continue this research by obtaining near infrared spectra (11001700nm) on hot-iron brands applied to 15 weaned (~ 270 d old; 238 ± 7kg) Bos taurus cross steers at 21, 32, and 51 d post-branding. Spectra were obtained on branded (n = 3) and unbranded (n = 3) skin tissue from each animal, each date. Hair was clipped to < 5mm on unbranded skin. Spectra were analyzed as log 1/reflectance with 1st derivative and scatter correction applied. Linear discriminant analysis and regression procedures were applied to distinguish between brand treatment and date post-branding. Spectra from branded and unbranded skin were correctly (P < 0.05) identified at ~95%. Brand age was predicted successfully (P < 0.05) but not accurately enough for forensic application (RSQ = 0.50; RMSE = 9.75 d). The NIRS technique can discriminate differences in the age of cattle brand scars but numerical prediction requires further investigation.
115 Safety in numbers: Social isolation increases behavioral responses of cattle during startle tests
Authors: Mathias Amanda , Daigle Courtney L 
Published: July 2019

Abstract
Social isolation confounds cattle fear tests. Fear tests often require individuals to be isolated from the herd, possibly eliciting other emotions (i.e. panic and grief) outside of fear. To test this, steer (n = 32) behavior during an individual startle test (IST) and a group startle test (GST) were video recorded. During IST, one steer was placed in a solid sided pen and, after 60 seconds, two umbrellas opened simultaneously. Behavior was video recorded for an additional four minutes. For GST, the same procedures were followed, except 4 familiar steers were in the pen simultaneously. Within each test, steers were evaluated in a random order. IST retesting (IST_2) occurred five weeks after the first IST (IST_1). The GST took place one week after IST_2 testing completed. Video recordings of IST_1, IST_2, and GST were decoded for frequency and duration of behavior (steps, standing bouts, time spent standing, eliminations). Relationships among behavior, test, and minute of test were analyzed using Generalized Linear Mixed Model (PROC MIXED). Minute and test affected number of steps (P < 0.0001) and time spent standing (P < 0.0001). During GST, steers performed fewer steps than in the IST_1 (P < 0.0001) and IST_2 (P = 0.0003). There were no differences in step count between IST_1 and IST_2. Steers spent more time standing during GST than IST_1 (P < 0.0001) and IST_2 (P = 0.004). No differences in time-spent standing were seen between IST_1 and IST_2. Number of standing bouts were impacted by test (P = 0.0008). Steers stood more during GST than in IST_1 (P = 0.0007) and IST_2 (PP = 0.03) with no differences observed between IST_1 and IST_2. The IST is repeatable, yet the strength of the behavioral response is reduced during GST. This is the first quantifiable representation of the impact of social isolation on the fear response in beef cattle.
123 Effect of a hydrolyzed yeast product on cow behavior, body eeight, body condition score, and hair coat score in cows grazing pastures containing endophyte-infected tall fescue
Authors: Harrelson Patricia L , Rogers C Brent , Martin Rick , Scott Johnna , Banks Brandi , McMillin Taelor , Sellinger Emory , Harrelson Flint W 
Published: July 2019

Abstract
The objective of this study was to determine if the addition of a hydrolyzed yeast product would alleviate the symptoms of fescue toxicosis in beef cattle. Thirty-eight Angus cow-calf pairs were stratified by cow age and body weight then randomly allotted to one of two treatments; control mineral (CON) or hydrolyzed yeast mineral (HYM). Cattle in both groups were allowed access to mineral for 126 d while they grazed the same eight pastures (1.21 ha each) in a rotational pattern. Mineral was provided at a target rate of 113.4 g·hd-1·d-1 every 28 d. Each pasture was grazed by cattle groups for 7 d. Mixed grass pastures containing tall fescue were utilized and evaluated for endophyte level prior to the start of the project. Due to pasture endophyte level variability (44%-73% endophyte), both groups of cattle rotationally grazed each pasture at least 2 times starting in June. Prior to entering a new pasture weekly, cows were weighed and assigned a BCS and hair coat score (HC) by two independent, trained personnel. Cow behavior was measured every 15-min within a 2-h block weekly. Percentage of cows active or inactive, outside or inside was recorded. Data were analyzed using the MIXED procedure of SAS. Cow BW change was unaffected by treatment (P > 0.23). Cow BCS significantly increased in HYM vs. CON cows (0.4 vs. 0.1; P = 0.02; SEM = 0.1) through the 126 d project. No treatment effect was observed on HC (P > 0.19). Cow behavior was impacted by treatment, as HYM cows spent more time outside compared to CON cows (P < 0.01). Results of this trial suggest that HYM cows may have experienced less heat stress as a result of fescue toxicosis as they were willing to spend more time outside and increased BCS.
122 The efficacy of extended-release eprinomectin for the reduction of horn flies, face flies, and fecal egg counts of parasitic nematodes in replacement beef heifers
Authors: Landers Sophia F , Tucker Christopher , Powell Jeremy G , Wray Eva , Cauble Reagan , Lester Toby D 
Published: July 2019

Abstract
The purpose of this study was to evaluate the efficacy of extended-release eprinomectin against horn flies, face flies, and fecal egg counts of parasitic nematodes in crossbreed replacement beef heifers. Fifty-four heifers were randomly placed into three treatment groups (n = 18 heifers/treatment). Group 1 was administered the labeled dosage of extended-release eprinomectin at the onset of fly season (day 0). Group 2 acted as the negative control. Group 3 received the anthelmintic injection once a quarter of the heifers in the group reached the threshold treatment level for horn flies (n = 200 flies/animal; day 41). All cattle were separately pastured for the duration of the study. Throughout the study, nematode infections were measured via fecal egg counts while horn and face flies were visually monitored. Evaluation of pregnancy status was recorded at the conclusion of the study (day 144). Due to the high number of face flies, heifers were treated with insecticide dusts twice during the study (day 54 and 69). Group 1 averaged a fecal egg count of (2.3), group 2 (7.4), and group 3 (9.1) (day 111). Low egg counts for all treatment groups reflected minimal parasite burden which likely had little effect on treatment groups. Extended release eprinomectin had little influence on face flies given that all groups were above threshold at days 40, 48, and 54. At study conclusion, horn fly population was lower (P < 0.05) for group 1 (405.6) compared to group 2 (680.6) and group 3 (680.6). Conception rates were greater (P < 0.05) in group 1 (89%) compared to the control heifers (56%) and group 3 exhibited an intermediate rate (72%). Results indicate that although treatment with extended-release eprinomectin did not decrease the face fly burdens, it did have an effect on fecal egg counts, horn fly burdens, and subsequent pregnancy status at the conclusion of the study
124 Finishing beef cattle under cover reduces heat load and improves efficiency during the summer
Authors: Sims William M , Stewart Lawton , Segers Jacob R , McKee Robert W , Rigdon Macc , Thomas Chevise L , Stelzleni Alexander M 
Published: July 2019

Abstract
Heat-stress in finishing cattle presents a significant risk to efficiency and economic viability. The project objective was to quantify the effects of long-term heat-stress when finishing cattle during the summer in the southeastern United States. Forty-five Angus crossbred steers (446±23 kg) were blocked by weight and randomly assigned to environmental finishing treatments including: covered with fan (CWF), covered without fan (CNF), or outside without shade (OUT). For 92 d steers were individually fed a typical feedlot ration. Environmental data were continuously recorded including: black globe temperature (BG), heat load index (HLI), and accumulated heat load units (AHLU). Feed intake was recorded daily, and steers were weighed every 20–25 days. When the first treatment averaged 613-kg all steers were slaughtered, and carcass data were collected. Data were analyzed as a Mixed Model (JMP V13; SAS Inst.) and means were separated (Least Squares Means). Average maximal BG was lower for covered finishing than OUT (P < 0.01); however, for HLI CWFCNF>OUT, while G:F was similar (P = 0.22) between CWF and CNF which were greater (P < 0.01) than OUT. Hot carcass weights were heavier for CWF than OUT (P < 0.01) and CNF was similar to both (P ≥ 0.11). There was no difference for USDA Yield Grade (2.6; P = 0.44), or marbling score (Modest20; P = 0.76). Steers finished under cover were more efficient than steers finished in open dry-lots. The addition of cooling fans further improved steer gains over those that were covered without fans.
126 Detection of fecal chemistry changes in cattle infested with the Southern Cattle Tick, Rhipicephalus (Boophilus) microplus
Authors: Rich Brian T , Teel Pete D , Thomas Donald B , Angerer Jay , Tolleson Douglas R , de Leon Adalberto A Perez 
Published: July 2019

Abstract
Bovine babesiosis, a highly fatal tick-borne disease of cattle, was eliminated from the United States in the last century through tick eradication programs against 2 species of cattle fever ticks. The threat to the U.S. cattle industry continues through tick introductions from Mexico. The standard method of detecting Cattle Fever Ticks [Rhipicephalus (B.) annulatus and R. (B.) microplus] in the state-federal tick eradication program is physical examination of restrained cattle to find attached ticks. New methods of detecting tick-infested cattle could improve reliability and reduce animal stress. The objective of this study was to determine whether changes in fecal chemistry induced by tick infestation was detectable using near-infrared reflectance spectroscopy (NIRS). Fecal samples were collected daily from 6 stanchioned Bos taurus yearling heifers (initial mean weight 163.3 kg +/- 4.7 kg) at the USDA Cattle Fever Tick Research Laboratory, Edinburg, TX, before, during, and after an infestation of 5000 R. (B.) microplus larvae. Cluster analyses were conducted using GRAMS IQ for NIR spectra in the 576–1126 nm range to test for fecal chemistry changes different from pre-infestation condition, and coincident with the biological phases of the tick infestation. The first three factors of spectral variation accounted for 87.87% of spectral variation among all samples. Factors 1, 2, and 3 had F-Ratios for the Reduced Eigenvalues of 941.59, 387.44, and 221.79, respectively. Three-dimensional analysis for these 3 factors shows shifts in sample clusters away from pre-infestation and coincident with progressive tick blood-feeding and post-infestation recovery. We conclude that fecal NIRS may provide a tool for detection of tick-infested cattle; however, further testing is needed to determine the sensitivity of detection on cattle with varying levels of tick burden, and a protocol developed and evaluated for fecal sampling under field conditions