bluuebunny/crossref_metadata_2025_split huggingface dataset:
Canine urinary bladder transitional cell carcinoma tumor volume is dependent on imaging modality and measurement technique
Authors: Leffler Andrew J. , Hostnik Eric T. , Warry Emma E. , Habing Gregory G. , Auld Danelle M. , Green Eric M. , Drost Wm Tod 
Published: July 2018

AbstractTransitional cell carcinoma is the most common cancer of the canine urinary tract. The inconsistent appearance of transitional cell carcinoma in patients introduces error if applying mathematic models for extrapolating total tumor volume from linear measurements. Reliable techniques to assess tumor size are important for monitoring treatment response. A method comparison study was performed comparing four techniques for calculating tumor volume were compared: (1 and 2) contoured tracing of tumor margins using serial computed tomography (CT) images using pre‐(1) and postintravenous (2) contrast medium studies, (3) longest three linear dimensions using CT, and (4) longest three linear dimensions on abdominal ultrasound. Volumes of the transitional cell carcinoma tumor calculated by CT tracing techniques were significantly smaller than volumes calculated with an ellipsoid mathematic model using the linear measurements (P < 0.01). Intravenous contrast medium did not significantly change the volumes calculated from tracing tumor margins on CT for observer B; however, volumes differed for observer A. The volumes extrapolated from linear measurements using CT and ultrasound did not differ significantly. The interobserver reliability was highest for the precontrast CT contoured technique and was lowest using the ultrasound linear technique. Tumor volumes differed significantly between techniques of contoured tracing of the tumor margins on serial CT images compared to calculation of tumor volume from linear dimensions. The calculated volume of a transitional cell carcinoma depends upon the technique used. Characterizing the response of urinary bladder transitional cell carcinoma tumor size to therapy differs based on the method and modality used.
Modeling the kinetics and cost performance index of high rate up‐flow anaerobic, anoxic, oxic bioreactor for meat wastewater treatment
Authors: Abyar Hajar , Younesi Habibollah , Bahramifar Nader , Zinatizadeh Ali Akbar 
Published: July 2018

AbstractThe simultaneous biological removal of COD, TN and phosphorus from meat industrial wastewater (MIW) using a lab‐scale high rate up‐flow anaerobic–anoxic–oxic bioreactor (A2O bioreactor) was investigated. The bioreactor was operated at different HRT, COD/TN ratio and aerated volume fractions to monitor the COD and nutrient removal efficiency. The maximum COD removal rate was detected at HRT of 5 h and COD/TN ratio of 100 : 12. The total nitrogen removal efficiency was changed between 65.8 and 94.4% and the lowest effluent nitrate concentration was measured as 1.6 mg/l. Seven kinetic models were applied to experimental data to determine the substrate utilization rate. The Monod and Haldane models were ideal to estimate the reactor performance. Moreover, the cost performance evaluation indicated the A2O bioreactor capability in low energy consumption for MIW treatment. According to the modeling results, integrating anaerobic and aerobic zones within a single bioreactor could be beneficial and cost‐effective due to the high effluent quality and low operational costs.
Real‐time optical manipulation of cardiac conduction in intact hearts
Authors: Scardigli M. , Müllenbroich C. , Margoni E. , Cannazzaro S. , Crocini C. , Ferrantini C. , Coppini R. , Yan P. , Loew L. M. , Campione M. , Bocchi L. , Giulietti D. , Cerbai E. , Poggesi C. , Bub G. , Pavone F. S. , Sacconi L. 
Published: July 2018

Key points
Although optogenetics has clearly demonstrated the feasibility of cardiac manipulation, current optical stimulation strategies lack the capability to react acutely to ongoing cardiac wave dynamics.
Here, we developed an all‐optical platform to monitor and control electrical activity in real‐time.
The methodology was applied to restore normal electrical activity after atrioventricular block and to manipulate the intraventricular propagation of the electrical wavefront.
The closed‐loop approach was also applied to simulate a re‐entrant circuit across the ventricle.
The development of this innovative optical methodology provides the first proof‐of‐concept that a real‐time all‐optical stimulation can control cardiac rhythm in normal and abnormal conditions.
AbstractOptogenetics has provided new insights in cardiovascular research, leading to new methods for cardiac pacing, resynchronization therapy and cardioversion. Although these interventions have clearly demonstrated the feasibility of cardiac manipulation, current optical stimulation strategies do not take into account cardiac wave dynamics in real time. Here, we developed an all‐optical platform complemented by integrated, newly developed software to monitor and control electrical activity in intact mouse hearts. The system combined a wide‐field mesoscope with a digital projector for optogenetic activation. Cardiac functionality could be manipulated either in free‐run mode with submillisecond temporal resolution or in a closed‐loop fashion: a tailored hardware and software platform allowed real‐time intervention capable of reacting within 2 ms. The methodology was applied to restore normal electrical activity after atrioventricular block, by triggering the ventricle in response to optically mapped atrial activity with appropriate timing. Real‐time intraventricular manipulation of the propagating electrical wavefront was also demonstrated, opening the prospect for real‐time resynchronization therapy and cardiac defibrillation. Furthermore, the closed‐loop approach was applied to simulate a re‐entrant circuit across the ventricle demonstrating the capability of our system to manipulate heart conduction with high versatility even in arrhythmogenic conditions. The development of this innovative optical methodology provides the first proof‐of‐concept that a real‐time optically based stimulation can control cardiac rhythm in normal and abnormal conditions, promising a new approach for the investigation of the (patho)physiology of the heart.
Nutritional Intake in Elite Cross-Country Skiers During Two Days of Training and Competition
Authors: Carr Amelia , McGawley Kerry , Govus Andrew , Andersson Erik P. , Shannon Oliver M. , Mattsson Stig , Melin Anna 
Published: July 2018

This study investigated the energy, macronutrient, and fluid intakes, as well as hydration status (urine specific gravity), in elite cross-country skiers during a typical day of training (Day 1) and a sprint skiing competition the following day (Day 2). A total of 31 (18 males and 13 females) national team skiers recorded their food and fluid intakes and urine specific gravity was measured on Days 1 and 2. In addition, the females completed the Low Energy Availability in Females Questionnaire to assess their risk of long-term energy deficiency. Energy intake for males was 65 ± 9 kcal/kg on Day 1 versus 58 ± 9 kcal/kg on Day 2 (p = .002) and for females was 57 ± 10 on Day 1 versus 55 ± 5 kcal/kg on Day 2 (p = .445). Carbohydrate intake recommendations of 10–12 g·kg−1·day−1 were not met by 89% of males and 92% of females. All males and females had a protein intake above the recommended 1.2–2.0 g/kg on both days and a postexercise protein intake above the recommended 0.3 g/kg. Of the females, 31% were classified as being at risk of long-term energy deficiency. In the morning of Day 1, 50% of males and 46% of females were dehydrated; on Day 2, this was the case for 56% of males and 38% of females. In conclusion, these data suggest that elite cross-country skiers ingested more protein and less carbohydrate than recommended and one third of the females were considered at risk of long-term energy deficiency. Furthermore, many of the athletes were dehydrated prior to training and competition.
Comparison of Body Composition Assessment Methods in Professional Urban Firefighters
Authors: Smee Disa J. , Walker Anthony , Rattray Ben , Cooke Julie A. , Serpell Ben G. , Pumpa Kate L. 
Published: July 2018

Given the importance of body composition in maintaining optimal physical and functional capacities, the use of appropriate, field-based assessment tools should be a priority to assist in maintaining the occupational safety of firefighters and the community. For ease, body mass index has often been used to assess these changes. However, it is limited in its accuracy. The purposes of this study were twofold: (a) to compare the validity of different measures of body composition against dual-energy X-ray absorptiometry (DXA) in urban firefighters and (b) to assess these measures in their ability to provide meaningful interpretation of criteria-driven categories of adiposity. A total of 64 male firefighters (age = 44.0 ± 9.5 years) underwent full anthropometric profiling (predictor equations used to determine body fat percentage [BF%]), bioelectrical impedance analysis (BIA), and DXA assessments. Participants’ body mass index was calculated, and BF% and lean mass were determined along with criteria-driven categorizations of adiposity. Anthropometric (skinfolds) predictor equations (e.g., mean bias = −4.4% for BF%) were typically closer to DXA measures, compared with BIA (9.4% for BF%). However, when determining categories of criteria-driven adiposity, BIA (42.9% overweight or obese) provided closer estimates to the DXA-determined distribution (44.6%) than anthropometric-based measures (up to 40%). Body mass index appears an inappropriate measure for accurately determining categories of adiposity with 64.1% classified as overweight or obese. Given the logistical constraints of anthropometric profiling, and the closeness of BIA to DXA in adiposity categories, BIA may be a suitable alternative to DXA for assessing body composition in professional urban firefighters.
Regulation of
Trypanosoma brucei
Acetyl Coenzyme A Carboxylase by Environmental Lipids
Authors: Ray Sunayan S. , Wilkinson Christina L. , Paul Kimberly S. 
Published: July 2018

Trypanosoma brucei
is a eukaryotic parasite that causes African sleeping sickness.
T. brucei
is transmitted by the blood-sucking tsetse fly. In order to adapt to its two very different hosts,
T. brucei
must sense the host environment and alter its metabolism to maximize utilization of host resources and minimize expenditure of its own resources. One key nutrient class is represented by fatty acids, which the parasite can either take from the host or make themselves. Our work describes a novel environmental regulatory pathway for fatty acid synthesis where the parasite turns off fatty acid synthesis when environmental lipids are abundant and turns on synthesis when the lipid supply is scarce. This pathway was observed in the tsetse midgut form but not the mammalian bloodstream form. However, pharmacological activation of this pathway in the bloodstream form to turn fatty acid synthesis off may be a promising new avenue for sleeping sickness drug discovery.
The 5′ Untranslated Region of the
EFG1
Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in
Candida albicans
Authors: Desai Prashant R. , Lengeler Klaus , Kapitan Mario , Janßen Silas Matthias , Alepuz Paula , Jacobsen Ilse D. , Ernst Joachim F. 
Published: July 2018

Many of the virulence traits that make
Candida albicans
an important human fungal pathogen are regulated on a transcriptional level. Here, we report an important regulatory contribution of translation, which is exerted by the extensive 5′ untranslated regulatory sequence (5′ UTR) of the transcript for the protein Efg1, which determines growth, metabolism, and filamentation in the fungus. The presence of the 5′ UTR is required for efficient translation of Efg1, to promote filamentation. Because transcripts for many relevant regulators contain extensive 5′ UTR sequences, it appears that the virulence of
C. albicans
depends on the combination of transcriptional and translational regulatory mechanisms.
Do the associations of sedentary behaviour with cardiovascular disease mortality and cancer mortality differ by physical activity level? A systematic review and harmonised meta-analysis of data from 850 060 participants
Authors: Ekelund Ulf , Brown Wendy J , Steene-Johannessen Jostein , Fagerland Morten Wang , Owen Neville , Powell Kenneth E , Bauman Adrian E , Lee I-Min 
Published: July 2018

Objective
To examine whether the associations between sedentary behaviours (ie, daily sitting/TV-viewing time) and mortality from cardiovascular disease (CVD) and cancer differ by different levels of physical activity (PA).

Design
Harmonised meta-analysis of prospective cohort studies. Data on exposure variables were harmonised according to a predefined protocol and categorised into four groups for sedentary behaviours and into quartiles of PA (MET-hour/week).

Data sources
PubMed, PsycINFO, Embase, Web of Science, Sport Discus and Scopus.

Eligibility criteria for selecting studies
Individual level data on both sedentary behaviours and PA and reported effect estimates for CVD or cancer mortality.

Results
Nine studies (n=850 060; deaths=25 730) and eight studies (n=777 696; deaths=30 851) provided data on sitting time and CVD and cancer mortality, respectively. Five studies had data on TV-viewing time and CVD (n=458 127; deaths=13 230) and cancer (n=458 091; deaths=16 430) mortality. A dose–response association between sitting time (9%–32% higher risk; p for trend <0.001) and TV time (3%–59% higher risk; p for trend <0.001) with CVD mortality was observed in the ‘inactive’, lowest quartile of PA. Associations were less consistent in the second and third quartiles of PA, and there was no increased risk for CVD mortality with increasing sedentary behaviours in the most active quartile. Associations between sedentary behaviours and cancer mortality were generally weaker; 6%–21% higher risk with longer sitting time observed only in the lowest quartile of PA.

Conclusion
PA modifies the associations between sedentary behaviours and CVD and cancer mortality. These findings emphasise the importance of higher volumes of moderate and vigorous activity to reduce, or even eliminate these risks, especially for those who sit a lot in their daily lives.
UDAY: A comprehensive diabetes and hypertension prevention and management program in India
Authors: Mohan Sailesh , Jarhyan Prashant , Ghosh Shreeparna , Venkateshmurthy Nikhil Srinivasapura , Gupta Ruby , Rana Ritu , Malhotra Cheena , Rao M Bhaskara , Kalra Sanjay , Tandon Nikhil , Srinath Reddy K , Prabhakaran Dorairaj 
Published: July 2018

IntroductionDiabetes and hypertension are two leading non-communicable conditions, which are suboptimally managed in India. Thus, innovative comprehensive approaches that can concomitantly improve their detection, prevention and control are warranted.Methods and analysisUDAY, a 5-year initiative, aims to reduce the risk of diabetes and hypertension and improve management by implementing a comprehensive intervention programme in the two selected study sites, Sonipat and Visakhapatnam (Vizag). It has a pre-post evaluation design with representative cross-sectional surveys before and after intervention. Within these two sites, urban and rural subsites each with a total population of approximately 100 000 people each were selected and a baseline and postintervention assessment was conducted deploying five surveys [among general population (including body measurements or biosamples), patients, healthcare providers including physicians and pharmacists, health facilities], which will determine the knowledge levels about diabetes and hypertension, the proportion treated and controlled; the patient knowledge and self-management skills; healthcare providers’ management practices; the level of access and barriers to obtaining care.The interventions will include: tailored health promotion for improving public knowledge; screening of adults aged ≥ 30 years for identifying those at high risk of diabetes and/or hypertension for linkage to the healthcare system; patient education using technology enabled community health workers, geographic information system (GIS) based mapping of the communities, healthcare provider training on management guidelines, community based diabetes registry and; advocacy to improve access to healthcare. The baseline surveys have been completed, the study areas mapped using GIS and the interventions are being implemented. UDAY is expected to increase over baseline the levels of: public knowledge about diabetes and hypertension; those treated and controlled; patient self-management skills; the use of guideline based management by providers and; access to healthcare, leading to improved health outcomes and inform development of a India relevant chronic care model.Ethics and disseminationEthical clearance for conduct of the study was obtained from the Institutional Ethics Committee (IEC) of the Public Health Foundation of India. The findings will be targeted primarily at public health policymakers and advocates, but will be disseminated widely through other mechanisms including conference presentations and peer-reviewed publications, as well as to the participating communities.
B Part of It protocol: a cluster randomised controlled trial to assess the impact of 4CMenB vaccine on pharyngeal carriage ofNeisseria meningitidisin adolescents
Authors: Marshall Helen S , McMillan Mark , Koehler Ann , Lawrence Andrew , MacLennan Jenny M , Maiden Martin C J , Ramsay Mary , Ladhani Shamez N , Trotter Caroline , Borrow Ray , Finn Adam , Sullivan Thomas , Richmond Peter , Kahler Charlene M , Whelan Jane , Vadivelu Kumaran 
Published: July 2018

IntroductionSouth Australia (SA) has the highest notification rate of invasive meningococcal disease in Australia with the majority of cases due to serogroup B.Neisseria meningitidisis carried in the pharynx, with adolescents having the highest rates of carriage. A vaccine designed to offer protection against serogroup B (4CMenB) is licensed in Australia. The SA MenB vaccine carriage study aims to assess the impact of 4CMenB on carriage ofN. meningitidisin adolescents.Methods and analysisThis is a parallel cluster randomised controlled trial enrolling year 10, 11 and 12 school students (approximately 16–18 years of age) throughout SA, in metropolitan and rural/remote areas. Schools are randomised to intervention (4CMenB vaccination at baseline) or control (4CMenB vaccination at study completion) with randomisation stratified by school size and socioeconomic status, as measured by the Index of Community Socio-Educational Advantage (Australian Curriculum). Oropharyngeal swabs will be taken from all students at visit 1, and 12 months later from year 11 and 12 students. Students unvaccinated in 2017 will receive vaccine at the 12-month follow-up. Carriage prevalence ofN. meningitidiswill be determined by PCR at baseline and 12 months following 4CMenB vaccination and compared with carriage prevalence at 12 months in unvaccinated students. A questionnaire will be completed at baseline and 12 months to assess risk factors associated with carriage. The primary outcome of carriage prevalence of disease causingN. meningitidisat 12 months will be compared between groups using logistic regression, with generalised estimating equations used to account for clustering at the school level. The difference in carriage prevalence between groups will be expressed as an OR with 95% CI.Ethics and disseminationThe study was approved by the Women’s and Children’s Health Network Human Research Ethics Committee (WCHN HREC). The protocol, informed consent forms, recruitment materials, social media and all participant materials have been reviewed and approved by the WCHN HREC and updated on ClinicalTrials.gov. Results will be published in international peer-reviewed journals and presented at national and international conferences. The study findings will be provided in public forums and to study participants and participating schools.Trial registration numberACTRN12617000079347.NCT03089086; Pre-results.
Association between proprotein convertase subtilisin/kexin type 9 and late saphenous vein graft disease after coronary artery bypass grafting: a cross-sectional study
Authors: Gao Jing , Wang Hai-Bo , Xiao Jian-yong , Ren Min , Reilly Kathleen Heather , Li Yu-Ming , Liu Yin 
Published: July 2018

ObjectiveThe study aims to explore the association between serum proprotein convertase subtilisin/kexin type 9 (PCSK9) level and saphenous vein grafts disease (SVGD) after coronary artery bypass grafting (CABG).DesignA cross-sectional study.SettingA secondary hospital in Tianjin City, China.ParticipantsA total of 231 participants were included in the study. Inclusion criteria were as follows: age ≥18 years, previous CABG surgery at least 12 months ago, at least one SVG for bypass during CABG, abnormal non-invasive test results or recurrent stable angina pectoris by coronary angiography indications, and willing to participate and sign informed consent. Participants with any of the following were excluded from the study: congenital valvular disease, decompensated heart failure, anaemia defined as a haemoglobin level of <12 g/dL in women or <13 g/dL in men, malignant neoplasms, renal failure, severe hepatic disease, thyroid disease, acute or chronic inflammatory disease and chronic obstructive lung disease.Primary outcome measureSVGD was defined as at least one SVG with significant stenosis (≥50%). Circulating PCSK9 levels were measured using commercial ELISA kits according to the manufacturer’s instructions.ResultsThe mean PCSK9 level in the SVGD group was significantly higher than that in the patent group (275.2±38.6 vs 249.3±37.7, p<0.01). The multivariate logistic regression model revealed a significant association between serum PCSK9 and SVGD (OR 2.08, 95% CI 1.46–2.95) per 1 SD increase in serum PCSK9.ConclusionsThe present study is the first to identify an independent association between PCSK9 and late SVGD after adjustment for established cardiovascular risk factors. A multicentre prospective cohort study with large sample size should be conducted in the future to further research this relationship.