bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of Apoptotic Circulating Tumor Cells Using in vivo Fluorescence Flow Cytometry
Authors: Nolan Jacqueline , Nedosekin Dmitry A. , Galanzha Ekaterina I. , Zharov Vladimir P. 
Published: December 2018

AbstractMost cancer patients die from metastatic disease as a result of a circulating tumor cell (CTC) spreading from a primary tumor through the blood circulation to distant organs. Many studies have demonstrated the tremendous potential of using CTC counts as prognostic markers of metastatic development and therapeutic efficacy. However, it is only the viable CTCs capable of surviving in the blood circulation that can create distant metastasis. To date, little progress has been made in understanding what proportion of CTCs is viable and what proportion is in an apoptotic state. Here, we introduce a novel approach toward in situ characterization of CTC apoptosis status using a multicolor in vivo flow cytometry platform with fluorescent detection for the real‐time identification and enumeration of such cells directly in blood flow. The proof of concept was demonstrated with two‐color fluorescence flow cytometry (FFC) using breast cancer cells MDA‐MB‐231 expressing green fluorescein protein (GFP), staurosporine as an activator of apoptosis, Annexin‐V apoptotic kit with orange dye color, and a mouse model. The future application of this new platform for real‐time monitoring of antitumor drug efficiency is discussed. © 2019 International Society for Advancement of Cytometry
Hair analysis to discriminate voluntary doping vs inadvertent ingestion of the aromatase inhibitor letrozole
Authors: Favretto Donata , Snenghi Rossella , Pertile Roberto , El Mazloum Rafi , Tucci Marianna , Visentin Sindi , Vogliardi Susanna 
Published: December 2018

AbstractLetrozole is an aromatase inhibitor, used to treat postmenopausal women with hormone receptor‐positive or unknown advanced breast cancer. It is prohibited in sport because it is used together with androgen anabolizing steroids to avoid their adverse effects. In the case of an adverse analytical finding, it may be important to distinguish between repetitive use due to voluntary administration and occasional use, possibly due to involuntary intake. With the objective to identify the dose capable of producing a positive hair test, and to apply these results to the scenarios of inadvertent letrozole ingestion by an athlete, this study investigates the urinary excretion and incorporation into hair of single doses of letrozole. Seven subjects were recruited for an excretion study of letrozole and its metabolite bis(4‐cyanophenyl) methanol (M1) in urine, after the consumption of 0.62 mg, 1.25 mg, and 2.5 mg of letrozole, and to investigate the incorporation in hair after ingestion of 0.62 mg and 2.5 mg of letrozole. Urine and hair samples were also obtained from two women in chronic therapy. Urinary concentrations of letrozole and its metabolite M1 were lower in subjects administered once with 0.62 mg, 1.25 mg, or 2.5 mg letrozole than in women in regular therapy with 2.5 mg/day. In hair collected after a single dosage, concentrations of 16–60 pg/mg were detected while in women in chronic therapy concentrations were higher than 160 pg/mg all along the hair shaft. Hair analysis turned to be a promising possibility for the discrimination of letrozole repetitive use vs occasional/inadvertent administration.
Ecosystem functions in mixed cropland–grassland systems influenced by soil legacies of past crop cultivation decisions
Authors: Turner Benjamin L. , Wuellner Melissa , Malo Douglas D. , Herrick Jeffrey E. , Dunn Barry H. , Gates Roger 
Published: December 2018

AbstractAs global food demand continues to grow, private landowners and agricultural managers have increased incentives to convert grasslands to expand crop production. These conversions are increasingly occurring on marginal soils susceptible to rapid degradation, which threatens delivery of diverse bundles of ecosystem goods and services (EGS). A growing number of studies have demonstrated that previous land management decisions continue to effect current soil ecosystem functions in the long‐term (i.e., soil legacies persist after previous management has ceased). Such legacies could further alterEGSdeliveries, especially in mixed‐use agroecosystems (grass and croplands) that are susceptible to large, rapid changes in land use. The objective of this work was therefore to identify potential soil legacy effects and recovery time delays after land transformation and to place those effects in the context ofEGStradeoffs. Our overall hypotheses were that soil legacies can be traced back to management'sEGSprioritization, that soil legacies persist due to the nature of land use (grass versus cultivation) and the time (years) under management, and that anthropomorphic manipulation from cultivation creates specific kinds of soil legacies. Using a systems approach that integrated ecosystem indicators, physical soil data, and human dimensions, we tested our hypotheses in South Dakota (USA), along the 100th Meridian (west), where recent and rapid cultivation expansion has reached historic highs. We conducted intensive interviews of four private land managers to identify historical land transformations, current goals, and strategies employed to achieve those goals on varying sites within their operations (nine grassland sites, nine cultivated sites;n = 18). Ecosystem assessments were conducted on each site using theUSinteragency assessment protocol Interpreting Indicators of Rangeland Health. Field aggregate stability and soil organic matter using loss‐on‐ignition were also measured. We found that (1) soil legacies continued to be detectable up to 20 yr after land transformation; (2) producers’ personal values ofEGSwere directly linked to observed land uses and ecosystem ratings; and (3) opportunities for reintroducing grasses into crop rotations or crop–livestock integration could likely improveEGSdelivery from converted lands while enhancing rural economic outcomes at a low‐ to no‐cost trade‐off between ecosystem functions.
Specificity of plant–plant communication for Baccharis salicifolia sexes but not genotypes
Authors: Moreira Xoaquín , Nell Colleen S. , Meza‐Lopez Maria M. , Rasmann Sergio , Mooney Kailen A. 
Published: December 2018

AbstractPlants are able to adjust their anti‐herbivore defenses in response to the volatile organic compounds (VOCs) emitted by herbivore‐damaged neighbors, and some of these changes increase resistance against subsequent herbivory. This phenomenon of plant–plant communication is thought to be widespread, but recent investigations have cautioned that it can be context dependent, including variation in the strength of communication based on the identity of plants and their associated herbivores. Here, we performed three greenhouse experiments using multiple male and female genotypes of the dioecious woody shrub Baccharis salicifolia and its specialist aphid Uroleucon macolai to test for specificity of plant–plant communication with respect to plant sex and genotype. Moreover, we evaluated plant sexual dimorphism and genotypic variation in VOC emissions (i.e., the “speaking” side of the interaction) and response of plants to VOC exposure (i.e., the “listening” side of the interaction) in order to identify the chemical mechanisms underlying such specificity. We did not find genotypic specificity of communication; emitter plants damaged by U. macolai significantly reduced subsequent U. macolai performance on receivers, but these effects were indistinguishable for communication within vs. among genotypes. In contrast, we found sex specificity of communication; male emitter plants reduced subsequent U. macolai performance on male and female receiver plants equally, while female emitter plants only did so for female receivers. We found sexual (but not genotypic) dimorphism in speaking but not listening; of the seven compounds induced by U. macolai feeding (speaking), pinocarvone was approximately fivefold greater in female than in male plants, while exposure of plants to pinocarvone emissions (listening) reduced U. macolai performance equally in both male and female plants. Together, our study demonstrates novel evidence for sexually dimorphic specificity of plant–plant communication and the chemical mechanism underlying this effect.
Quantification of bedform dynamics and bedload sediment flux in sandy braided rivers from airborne and satellite imagery
Authors: Strick Robert J.P. , Ashworth Philip J. , Sambrook Smith Gregory H. , Nicholas Andrew P. , Best James L. , Lane Stuart N. , Parsons Daniel R. , Simpson Christopher J. , Unsworth Christopher A. , Dale Jonathan 
Published: December 2018

AbstractImages from specially‐commissioned aeroplane sorties (manned aerial vehicle, MAV), repeat unmanned aerial vehicle (UAV) surveys, and Planet CubeSat satellites are used to quantify dune and bar dynamics in the sandy braided South Saskatchewan River, Canada. Structure‐from‐Motion (SfM) techniques and application of a depth‐brightness model are used to produce a series of Digital Surface Models (DSMs) at low and near‐bankfull flows. A number of technical and image processing challenges are described that arise from the application of SfM in dry and submerged environments. A model for best practice is presented and analysis suggests a depth‐brightness model approach can represent the different scales of bedforms present in sandy braided rivers with low‐turbidity and shallow (< 2 m deep) water.The aerial imagery is used to quantify the spatial distribution of unit bar and dune migration rate in an 18 km reach and three ~1 km long reaches respectively. Dune and unit bar migration rates are highly variable in response to local variations in planform morphology. Sediment transport rates for dunes and unit bars, obtained by integrating migration rates (from UAV) with the volume of sediment moved (from DSMs using MAV imagery) show near‐equivalence in sediment flux. Hence, reach‐based sediment transport rate estimates can be derived from unit bar data alone. Moreover, it is shown that reasonable estimates of sediment transport rate can be made using just unit bar migration rates as measured from 2D imagery, including from satellite images, so long as informed assumptions are made regarding average bar shape and height. With recent availability of frequent, repeat satellite imagery, and the ease of undertaking repeat MAV and UAV surveys, for the first time, it may be possible to provide global estimates of bedload sediment flux for large or inaccessible low‐turbidity rivers that currently have sparse information on bedload sediment transport rates. © 2018 The Authors. Earth Surface Processes and Landforms published by John Wiley & Sons Ltd.
Effects of grain‐size distribution and shape on sediment bed stability, near‐bed flow and bed microstructure
Authors: Staudt Franziska , Mullarney Julia C. , Pilditch Conrad A. , Huhn Katrin 
Published: December 2018

AbstractDifferent studies investigating the stability of mixed sediment have found that the fine fraction can either stabilize or mobilize the bed. This study aims to find where the transition between these two modes occurs for sandy sediment and to identify the underlying (grain‐scale) processes. Flume experiments with bimodal sediment were used to investigate near‐bed processes of a non‐cohesive sediment bed, and in particular how the grain shape and the ratio of different grain sizes influence bed mobility. Medium sand (D50,c ≈ 400 μm) was mixed with 40 % fine material of different diameters (D50,f = 53; 111; 193 μm) and subjected to increasing flow velocities (U = 1.3–22.2 cm s‐1). The bed mobility (i.e. the change of the bed level over time), turbidity and near‐bed hydrodynamics were analysed. Selected results were compared with similar previous experiments with spherical glass beads. The findings indicate that, due to the complex grain shapes of natural sediment, a sand bed is more stable than a bed composed of glass beads. The grain‐size ratio RD = Dc /Df between the coarse and fine grain diameters controls whether the mixed bed is stabilized or mobilized by the presence of fines, with the transition between the modes occurring at RD = 4–5.5. Mixed beds with a very low RD < 2 behave like a unimodal bed. The results suggest that RD and grain shape influence bed roughness, near‐bed flow, bed microstructure and the flow into and through the upper bed layers, which subsequently governs bed mobility. The interplay between all these processes can explain the transition between the stabilizing effect (high RD, small pore space) and the mobilizing effect (low RD, large pore space) of a fine fraction in a grain‐size mixture. © 2018 John Wiley & Sons, Ltd.
Target site model: Predicting mode of action and aquatic organism acute toxicity using Abraham parameters and feature-weighted k-nearest neighbors classification
Authors: Boone Kathleen S. , Di Toro Dominic M. 
Published: December 2018

Abstract

A database of 1480 chemicals with 47 associated modes of action compiled from the literature encompasses a wide range of chemical classes (alkanes, polycyclic aromatic hydrocarbons, pesticides, and polar compounds) and includes toxicity data for 79 different aquatic genera. The data were split into a calibration group and a validation group (80/20) to apply k-nearest neighbors (k-NN) methodology to predict the toxic mode of action for the compound. Other approaches were tested (support vector machines and linear discriminant analysis) as well as variations in the k-NN technique (distance weighting, feature weighting). Best-prediction results were found with k = 3, in a voting platform with optimized feature weighting. Using the predicted mode of action, the appropriate polyparameter target site model for that mode of action is applied to calculate the 50% lethal concentration (LC50). Predicted LC50s for the validation database resulted in a root-mean squared error (RMSE) of 0.752. This can be compared to an RMSE of 0.655 for the same validation set using the reference mode of action labels. The complete database resulted in an RMSE of 0.793 for reference mode of action labels. This confirms that the classification model has sufficient accuracy for predicting the mode of action and for determining toxicity using the target site model. Environ Toxicol Chem 2019;38:375–386. © 2018 SETAC
Synthesis of Ferrocene Derivatives Allowing Linear Free Energy Studies of Redox Potentials
Authors: Lim Byung Joon , Hwang Inhong , Ellington Andrew D. , Sessler Jonathan L. 
Published: December 2018

AbstractA series of ferrocene derivatives, which have diverse redox potentials modulated by functional groups, have been synthesized as potential ‘multi‐potential’ probes. A Hammett constant analysis revealed a linear free energy correlation between the redox potentials and the electron density of the ferrocene derivatives as determined by the choice of functional group used to modify the ferrocene core.
Numerical simulation of turbulent natural convection of nanofluid with thermal radiation inside a tall enclosure under the influence of magnetohydrodynamic
Authors: Rahmati Ahmad Reza , Sepehrnia Mojtaba , Motamedian Mahdi 
Published: December 2018

AbstractIn the present study, the natural convective heat transfer in the turbulent flow of water/CuO nanofluid with volumetric radiation and magnetic field inside a tall enclosure has been numerically investigated. The thermophysical properties of nanofluid have been considered variable with temperature and the effects of Brownian motion of nanoparticles have been considered. The main objective of this work is an investigation of the effect of using water/CuO nanofluid and presence of magnetic field on turbulent natural convection in three types of enclosures (vertical, inclined, and horizontal) by considering the volumetric radiation. The governing equations on turbulent flow domain under the influence of the magnetic field and by considering the combination of volumetric radiation and natural convection have been solved by a coupled algorithm. For validating the present research, a comparison has been carried out with the laminar natural convection flow under the influence of the magnetic field and radiation effects and also, the natural turbulent convection flow of previous studies and a proper coincidence has been achieved. The results indicated that by increasing volume fraction and Hartmann number the average Nusselt number enhances and reduces, respectively. By adding 1% CuO nanoparticles to the base fluid, heat transfer improves from 10.59% to 17.05%. However, by increasing the volume fraction from 1% to 4%, heat transfer improves from 1.35% to 4.90%. By increasing Hartmann number from 0 to 600, heat transfer reduces from 9.29% to 22.07%. Also, the results show that the ratio of deviation angle of the enclosure to the horizontal surface has considerable effects on heat transfer performance. Therefore, in similar conditions, the inclined enclosure with a deviation angle of 45° compared to the vertical and horizontal enclosure has better thermal performance.
Adomian decomposition solution for propulsion of dissipative magnetic Jeffrey biofluid in a ciliated channel containing a porous medium with forced convection heat transfer
Authors: Manzoor Naeema , Maqbool Khadija , Bég Osman Anwar , Shaheen Sidra 
Published: December 2018

AbstractPhysiological transport phenomena often feature ciliated internal walls. Heat, momentum, and multispecies mass transfer may arise and additionally non‐Newtonian biofluid characteristics are common in smaller vessels. Blood (containing hemoglobin) or other physiological fluids containing ionic constituents in the human body respond to magnetic body forces when subjected to external (extracorporeal) magnetic fields. Inspired by such applications, in the present work we have considered the forced convective flow of an electrically conducting viscoelastic physiological fluid through a ciliated channel under the action of a transverse magnetic field. The presence of deposits (fats, cholesterol, etc.) in the channel is mimicked with a Darcy porous medium drag force model. The effect of energy loss is simulated via the inclusion of viscous dissipation in the energy conservation (heat) equation. The velocity, temperature, and pressure distribution are computed in the form of infinite series constructed by Adomian decomposition method and numerically evaluated in a symbolic software (Mathematica). The influence of Hartmann number (magnetic parameter), Jeffrey first and second viscoelastic parameters, permeability parameter (modified Darcy number), and Brinkman number (viscous heating parameter) on velocity, temperature, pressure gradient, and bolus dynamics is visualized graphically.
Impact of the Cattaneo‐Christov thermal and solutal diffusion models on the stagnation point slip flow of Walters' B nanofluid past an electromagnetic sheet
Authors: Nayak M. K. , Oyelakin I. S. , Mondal S. , Sen S. S. 
Published: December 2018

AbstractThe present study investigates the effect of Cattaneo‐Christov thermal and solutal diffusion on the stagnation point flow of Walters' B nanofluid past an electromagnetic sheet subject to velocity, thermal, and solutal slips. The study analyzed the role of electromagnetic fields. In addition, the authors introduced the heat transfer aspect due to Brownian motion and thermophoretic force. The numerical solution of the transformed governing equations employed the spectral local linearization method. Comparisons showed an excellent agreement with the numerical data presented in previous notable works. The study reveals that the developed electromagnetic field due to the arrangement of the sheet causes accelerated fluid motion, and diminution of nondimensional temperature and concentration. In addition, augmented velocity, thermal, and solutal slips develop the corresponding descending boundary layers. The augmented short memory coefficient enhances the skin friction coefficient. The Cattaneo‐Christov thermal and solutal diffusion upsurge the heat and mass transfer rates from the electromagnetic sheet, respectively.
Nonlinear thermal radiation and chemical reaction effects on Maxwell fluid flow with convectively heated plate in a porous medium
Authors: Hosseinzadeh Kh. , Gholinia M. , Jafari B. , Ghanbarpour A. , Olfian H. , Ganji D. D. 
Published: December 2018

AbstractThis paper investigated the chemically reactive radiating flow by using a two‐dimensional Darcy‐Forchheimer model with the convectively heated plate. The nonlinear thermal radiation is described by Joule heating and heat generation. Also, Darcy‐Forchheimer equation is related to porous medium flows. For the solution of equations, we used the numerical method. Further, more physical interpretation of the parameters was demonstrated with figures. It is found that an increase in the Prandtl number had a direct effect on the Nusselt number and temperature, whereas the opposite scenario was observed in the Eckert number.
Sensitivity analysis of the effects of the thermal parameters of exhaust gases on the output of a thermoelectric generator
Authors: Mostafavi Seyed Alireza , Taheri Moein , Mahmoudi Mojtaba 
Published: December 2018

AbstractRecovering the dissipated heat from exhaust is a useful means of reducing the energy consumption level as well as cutting down on environmental pollution. An efficient technique for recovering this lost heat is the use of thermoelectric generators, which directly convert the dissipated heat into useful electrical energy. In this paper, a whole thermoelectric generator system installed on the exhaust pipe of a vehicle has been modeled, and the effects of thermal parameters on the output of this thermoelectric generator have been measured and evaluated by means of sensitivity analysis. The E‐Fast sensitivity analysis method has been used in this study. The sensitivity analysis results indicate that, among the thermal parameters examined, the temperature of gases entering the heat sink installed at exhaust pipe outlet () has the greatest influence (37%) and the flow rate of fluid entering the heatsink installed on the cold side of thermoelectric modules () has the second greatest influence (17%) on the output power of the considered thermoelectric generator. By using these results, the best cases of hot exhaust gases from various industries and vehicles with the highest potential of recovering the dissipated energy and heat from them by thermoelectric generators can be identified.
Correlation of abrin‐mediated inhibition of protein synthesis and apoptosis
Authors: Tiwari Vinita , Karande Anjali A. 
Published: December 2018

AbstractThe plant toxin, abrin, a type‐II ribosome inactivating protein, is extremely lethal, the human fatal dose being ~1 μg/kg body weight. Abrin has been classified as an agent for bioterrorism, which is of concern. Conversely, the high toxic property of abrin has been employed in generating immunotoxins, whereas its toxin moiety is conjugated to cell surface marker‐specific antibodies for cell‐targeted killing. Different cell types exhibit variable levels of sensitivity to abrin toxicity; therefore, adequate knowledge of the molecular mechanism that governs the activity of the protein would be a safeguard. To gain insights into this, two cell lines requiring strikingly different concentrations of abrin for inactivating ribosomes were studied. Employing conjugates of the wild‐type and active site mutant of abrin A chain with the ricin B chain, it was found that abrin‐induced apoptosis was dependent on inhibition of protein synthesis (PSI) leading to ER‐stress in Ovcar‐3 cells, but not in KB cells. Abrin was also observed to cause direct DNA damage in KB cells, while in Ovcar‐3 cells abrin‐induced DNA damage was found to be dependent on caspases. Overall, the study demonstrates that the correlation of abrin‐mediated PSI and apoptosis is cell‐specific and abrin can induce more than one pathway to cause cell death. © 2018 IUBMB Life, 71(3):357–363, 2019