bluuebunny/crossref_metadata_2025_split huggingface dataset:
MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR‐155
Authors: Li Zhenzhen , Han Shichao , Jia Yanhui , Yang Yunshu , Han Fu , Wu Gaofeng , Li Xiaoqiang , Zhang Wei , Jia Wenbin , He Xiang , Han Juntao , Hu Dahai 
Published: February 2019

AbstractLiver injury plays vital roles in the development of inflammation and organ dysfunction during sepsis. MCP‐1‐induced protein 1 (MCPIP1), as an endoribonuclease, is a critical regulator for the maintenance of immune homeostasis. However, whether MCPIP1 participates in the septic liver injury remains unknown. The aim of this study was to investigate the role of MCPIP1 in lipopolysaccharides‐induced liver injury and the underlying modulatory mechanisms. Quantitative real‐time polymerase chain reaction and immunoblotting were used to determine proinflammatory cytokines, MCPIP1, retinoid‐related orphan receptor α (RORα), miR‐155, and related protein from nuclear factor‐κB (NF‐κB) pathway expression. Dual luciferase reporter assay was used to analyze whether miR‐155 regulates RORα transcription. Secretion of inflammatory cytokines into sera in mice were measured by enzyme‐linked immunosorbent assay. Hematoxylin and eosin staining, alanine aminotransferase, and aspartate transaminase, assay were used to evaluate liver function. We found that MCPIP1 expression was notably upregulated and significantly downregulated inflammatory cytokine secretion and NF‐κB signaling activation in macrophages following exposure to lipopolysaccharide. Moreover, miR‐155, lowered by MCPIP1, directly targeted on 3′‐untranslated region of RORα to activate an inflammatory response. Importantly, MCPIP1 overexpression in mice alleviated septic liver injury symptoms following lipopolysaccharides stimulation. Collectively, these data highlight MCPIP1/miR‐155/RORα axis as a novel modulation of inflammation in liver injury and potential therapeutic target for future research.
Gene therapy in rheumatoid arthritis: Strategies to select therapeutic genes
Authors: Zavvar Mahdi , Assadiasl Sara , Soleimanifar Narjes , Pakdel Fatemeh Dadgar , Abdolmohammadi Kamal , Fatahi Yousef , Abdolmaleki Mohsen , Baghdadi Hamed , Tayebi Lobat , Nicknam Mohammad H. 
Published: February 2019

AbstractSignificant advances have been achieved in recent years to ameliorate rheumatoid arthritis (RA) in animal models using gene therapy approaches rather than biological treatments. Although biological agents serve as antirheumatic drugs with suppressing proinflammatory cytokine activities, they are usually accompanied by systemic immune suppression resulting from continuous or high systemic dose injections of biological agents. Therefore, gene transfer approaches have opened an interesting perspective to deliver one or multiple genes in a target‐specific or inducible manner for the sustained intra‐articular expression of therapeutic products. Accordingly, many studies have focused on gene transferring methods in animal models by using one of the available approaches. In this study, the important strategies used to select effective genes for RA gene therapy have been outlined. Given the work done in this field, the future looks bright for gene therapy as a new method in the clinical treatment of autoimmune diseases such as RA, and by ongoing efforts in this field, we hope to achieve feasible, safe, and effective treatment methods.
Surgical management of colorectal lung metastases
Authors: Phillips Joseph D. , Hasson Rian M. 
Published: February 2019

AbstractPulmonary metastasectomy for colorectal cancer is an established means of treatment for select patients. This article will highlight the recent evidence published in the literature related to current practices for the surgical management of colorectal lung metastases and propose a diagnostic algorithm for use in clinical practice. It will also discuss controversies related to pulmonary metastasectomy, including the optimal timing of surgery, the extent of lymph node sampling/dissection, and the extent of surgical resection.
Submicellar liquid chromatography with fluorescence detection improves the analysis of naproxen in plasma and brain tissue
Authors: García‐Herrero Víctor , Torrado‐Salmerón Carlos , García‐Rodríguez Juan José , Torrado Guillermo , Torrado‐Santiago Santiago 
Published: February 2019

AbstractRapid, simple, and sensitive submicellar liquid chromatography with fluorescence detection was developed and validated to quantify naproxen in plasma and brain samples after oral administration of Naproxen formulations. The method used tramadol as an internal standard. Different submicellar mobile phases with organic phases ranging from 40 to 60% were studied to improve the native fluorescence of the Naproxen and decrease retention times. Separation was done in a Zorbax SB C8 column (250 × 4.6 mm, 5 μm) with a mobile phase containing acidic 0.007 M sodium dodecyl sulfate/acetonitrile (50:50, v/v) at a flow rate of 1 mL/min. Detection was performed with an excitation wavelength of 280 nm and emission of 310 nm and 360 nm for internal standard and Naproxen, respectively. The method was validated by International Conference of Harmonization standards. The method is specific, accurate, and precise (relative standard deviation <3%). Limits of detection and quantification were 0.08 and 0.25 μg/mL, respectively, for biological samples. This method was applied to analyze brain/plasma ratios in mice that had received oral administrations of Naproxen micellar formulations containing 10% w/w of sodium dodecyl sulfate, Cremophor RH 40, or Tween 80. The sodium dodecyl sulfate micelles were faster and more widely distributed in the mouse brains.
Improvement in nasal obstruction and quality of life after septorhinoplasty and turbinate surgery
Authors: Yamasaki Alisa , Levesque Patricia A. , Bleier Benjamin S. , Busaba Nicolas Y. , Gray Stacey T. , Holbrook Eric H. , Sedaghat Ahmad R. , Lindsay Robin W. 
Published: February 2019

ObjectivesTo evaluate the long‐term impact of functional septorhinoplasty (SRP) with and without inferior turbinate reduction (ITR) on disease‐specific symptom severity and general health‐related quality of life (QOL).Study DesignProspective cohort study at a tertiary referral center.MethodsPatients undergoing functional SRP with and without ITR were administered the Nasal Obstruction Symptom Evaluation (NOSE) scale to assess severity of nasal obstruction and the EuroQol‐5 Dimension Questionnaire Visual Analog Scale (EQ‐5D VAS) to assess general health‐related QOL preoperatively and at 2, 4, 6, 12, 24, and 36 months postoperatively. Patient demographics, surgical technique, symptom severity, and QOL outcomes were analyzed.ResultsA total of 567 patients were included, with 391 patients undergoing functional SRP alone (54.0% female; mean age 36.0 years [standard deviation (SD):16.2]) and 176 patients undergoing functional SRP with ITR (50.0% female; mean age 35.6 years [SD:13.6]). There was a significant decrease in NOSE and increase in EQ‐5D VAS scores in both groups through at least 24 months postoperatively. Change in NOSE scores was negatively correlated with change in EQ‐5D VAS (r = −0.38, P < 0.01). Compared to patients undergoing SRP, patients also undergoing ITR had a statistically but nonclinically significant improvement in NOSE, with similar trends for EQ‐5D VAS that were not significant.ConclusionSRP results in a sustained, long‐term improvement in nasal obstruction based on disease‐specific and general health‐related QOL measures, with incremental improvement in outcomes with addition of ITR. This study provides the foundation for defining health outcomes and the health utility value of surgical interventions that address nasal obstruction.Level of Evidence2cLaryngoscope, 129:1554–1560, 2019
Single, high‐dose local injection of bFGF improves thyroarytenoid muscle atrophy after paralysis
Authors: Goto Takao , Ueha Rumi , Sato Taku , Fujimaki Yoko , Nito Takaharu , Yamasoba Tatsuya 
Published: February 2019

Objectives/HypothesisUnilateral vocal fold paralysis (UVFP) induces hoarseness due to progressive atrophy of the denervated thyroarytenoid (TA) muscle. Therefore, treatments aimed at regenerating the atrophied TA muscle are required. Basic fibroblast growth factor (bFGF) is involved in muscle development and regeneration. This study aimed to elucidate the effects of bFGF injection on atrophied TA muscle.Study DesignAnimal research.MethodsA recurrent laryngeal nerve–paralysis rat model was established, and low‐ (200 ng) or high‐dose (2,000 ng) bFGF or saline (control) was injected into the TA muscle 28 days later. The larynges were excised on day 1, 3, 7, 14, and 28 after treatment. The cross‐sectional area of the TA muscle in normal and paralyzed sides was compared, and the Ki67‐positive (Ki67+) dividing cells, paired box 7–positive (Pax7+) satellite cells (SCs), and myogenic differentiation–positive (MyoD+) myoblasts were counted.ResultsThe TA muscle area of animals administered high‐dose bFGF increased with time and was significantly larger than that of the saline‐injected controls 28 days after treatment (P < .05). The counts of Ki67+ and Pax7+ cells were the highest on day 1, whereas the MyoD+ myoblast count was highest on day 7. These results suggest that bFGF administration into the denervated TA muscles compensated for the atrophied TA muscles by inducing proliferation of SCs and their differentiation to myoblasts.ConclusionsA single injection of high‐dose bFGF augmented regeneration and differentiation of the atrophied TA muscle by enhancing proliferation and differentiation of muscle SCs, suggesting its possible clinical application in humans with UVFP.Level of EvidenceNA Laryngoscope, 130:159–165, 2020
Near‐infrared multispectral endoscopic imaging of deep artificial interproximal lesions in extracted teeth
Authors: Zhou Yaxuan , Lee Robert C. , Finkleman Sara , Sadr Alizera , Seibel Eric J. 
Published: February 2019

Background and ObjectiveA safer alternative method to radiographic imaging is needed. We present a multispectral near‐infrared scanning fiber endoscope (nirSFE) for dental imaging which is designed to be the smallest imaging probe with near‐infrared (NIR) imaging (1200–2000 nm).Materials and MethodsThe prototype nirSFE is designed for wide‐field forward viewing of scanned laser illumination at 1310, 1460, or 1550 nm. Artificial lesions with varying sizes and locations were prepared on proximal surfaces of extracted human teeth to examine capability and limitation of this new dental imaging modality. Nineteen artificial interproximal lesions and several natural occlusal lesions on extracted teeth were imaged with nirSFE, OCT, and microCT.ResultsOur nirSFE system has a flexible shaft as well as a probe tip with diameter of 1.6 mm and a rigid length of 9 mm. The small form factor and multispectral NIR imaging capability enables multiple viewing angles and reliable detection of lesions that can extend into the dentin. Among nineteen artificial interproximal lesions, the nirSFE reflectance imaging operating at 1460‐nm and OCT operating at 1310‐nm scanned illumination exhibited high sensitivity for interproximal lesions that were closer to occlusal surface. Diagnosis from a non‐blinded trained user by looking at real‐time occlusal‐side nirSFE videos indicate true positive rate of 78.9%. There were no false positives.ConclusionsThis study demonstrates that nirSFE may be used for detecting occlusal lesions and interproximal lesions located less than 4 mm under the occlusal surface. Major advantages of this imaging system include multiple viewing angles due to flexibility and small form factor, as well as the ability to capture real‐time video. The multispectral nirSFE has the potential to be employed as a low‐cost dental camera for detecting dental lesions without exposure to ionizing radiation. Lasers Surg. Med. 51:459–465, 2019. © 2019 Wiley Periodicals, Inc.
Age differences in photodynamic therapy‐mediated opening of the blood‐brain barrier through the optical clearing skull window in mice
Authors: Zhang Chao , Feng Wei , Li Yusha , Kürths Jurgen , Yu Tingting , Semyachkina‐Glushkovskaya Oxana , Zhu Dan 
Published: February 2019

BackgroundPhotodynamic therapy (PDT), a minimally invasive therapeutic tool, has been an important option for post‐surgical treatment of malignant gliomas (MGs) in both adult and young patients. Recent studies have shown that PDT can also open the blood‐brain barrier (BBB). However, there are no optimized parameters of PDT for patients at different ages. To determine whether there are age differences in PDT effects on the BBB, we studied PDT‐related BBB opening through the optical clearing skull window in healthy 4‐ and 8‐week‐old mice.MethodsIn this work, we realized BBB opening by combining PDT with the optical clearing skull window by using different radiant exposures (635 nm, 10–20–30–40 J/cm2) and 5‐aminole‐vulinic acid (5‐ALA, 20 mg/kg). Then, we evaluated BBB permeability by: (i) spectrofluorimetric measuring of Evans Blue dye (EBd) leakage; (ii) confocal imaging of 70 kDa FITC‐dextran extravasation and the BBB integrity; and (iii) histological analysis of brain tissues.ResultsUsing the skull optical clearing method, we demonstrated PDT‐induced BBB opening to EBd and FITC‐dextran in a radiant exposure manner. The histological analysis revealed the different severities of vasogenic edema corresponding to radiant exposures. Besides, the PDT‐related increase in the BBB permeability to high weight molecules (EBd and FITC‐dextran) and solutes (vasogenic edema) was more pronounced in 4‐week‐old mice than in 8‐week‐old mice.ConclusionsThe more pronounced PDT‐induced BBB disruption in juvenile mice compared with adult mice suggests age differences in PDT‐related BBB opening. This might be an important informative platform for a new application of PDT as a method for brain drug delivery, especially for post‐surgical treatment of MGs. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.
Surface Modification of Multiple Bioactive Peptides to Improve Endothelialization of Vascular Grafts
Authors: Peng Ge , Yao Danyu , Niu Yimeng , Liu Haifeng , Fan Yubo 
Published: February 2019

AbstractEndothelialization is an effective approach to prevent thrombus formation and enhance vascular graft survival. Surface modification of biomolecules has been proved to be effective in regulating endothelial cell behaviors. In this study, several peptides including YIGSR, RGD, and REDV sequences are covalently immobilized on the surface of electrospun silk fibroin scaffolds and the effects of combined application of these peptides on cell behaviors are studied. The results show that, compared with the scaffolds modified with single peptides, the scaffolds modified with dual peptides (YIGSR+RGD) could significantly enhance the proliferation of human umbilical vein endothelial cells (HUVECs). However, the combination of REDV+RGD or YIGSR+REDV does not promote the adhesion or proliferation of HUVECs. Notably, YIGSR‐modified scaffolds improved HUVEC migration significantly in comparison to REDV‐ or RGD‐modified groups. Moreover, its combination with either of these two peptides also presents excellent effect on cell migration. Thus, all the data suggest that the combined application of peptides might be a promising method to enhance the endothelialization of small‐diameter vascular grafts.
Comparative genomics of Paraburkholderia kururiensis and its potential in bioremediation, biofertilization, and biocontrol of plant pathogens
Authors: Dias Graciela M. , de Sousa Pires Araceli , Grilo Vinicius S. , Castro Michele R. , de Figueiredo Vilela Leonardo , Neves Bianca C. 
Published: February 2019

AbstractBurkholderia harbors versatile Gram‐negative species and is β‐Proteobacteria. Recently, it was proposed to split the genus in two main branches: one of animal and plant pathogens and another, Paraburkholderia, harboring environmental and plant‐beneficial species. Currently, Paraburkholderia comprises more than 70 species with ability to occupy very diverse environmental niches. Herein, we sequenced and analyzed the genome of Paraburkholderia kururiensis type strain KP23T, and compared to P. kururiensis M130, isolated in Brazil, and P. kururiensis susbp. thiooxydans, from Korea. This study focused on the gene content of the three genomes with special emphasis on their potential of plant‐association, biocontrol, and bioremediation. The comparative analyses revealed several genes related to plant benefits, including biosynthesis of IAA, ACC deaminase, multiple efflux pumps, dioxygenases, and degradation of aromatic compounds. Importantly, a range of genes for protein secretion systems (type III, IV, V, and VI) were characterized, potentially involved in P. kururiensis well documented ability to establish endophytic association with plants. These findings shed light onto bacteria‐plant interaction mechanisms at molecular level, adding novel information that supports their potential application in bioremediation, biofertilization, and biocontrol of plant pathogens. P. kururiensis emerges as a promising model to investigate adaptation mechanisms in different ecological niches.
Asymptotic analysis for Cahn–Hilliard type phase‐field systems related to tumor growth in general domains
Authors: Kurima Shunsuke 
Published: February 2019

This article considers a limit system by passing to the limit in the following Cahn–Hilliard type phase‐field system related to tumor growth as β↘0:

urn:x-wiley:mma:media:mma5520:mma5520-math-0001
in a bounded or an unbounded domain
with smooth‐bounded boundary. Here,
, T > 0, α > 0, β > 0, p ≥ 0, B is a maximal monotone graph, and π is a Lipschitz continuous function. In the case that Ω is a bounded domain, p and −Δ + 1 are replaced with p(φβ) and −Δ, respectively, and p is a Lipschitz continuous function; Colli, Gilardi, Rocca, and Sprekels (Discrete Contin Dyn Syst Ser S 2017; 10:37–54) have proved existence of solutions to the limit problem with this approach by applying the Aubin–Lions lemma for the compact embedding H1(Ω)↪L2(Ω) and the continuous embedding L2(Ω)↪(H1(Ω))∗. However, the Aubin–Lions lemma cannot be applied directly when Ω is an unbounded domain. The present work establishes existence of weak solutions to the limit problem along with uniqueness and error estimates in terms of the parameter β↘0. To this end, we construct an applicable theory by noting that the embedding H1(Ω)↪L2(Ω) is not compact in the case that Ω is an unbounded domain.
A compact W‐band mixer‐first receiver in 65 nm CMOS
Authors: Nam Hyohyun , Go Dong‐Sik , Park Jung‐Dong 
Published: February 2019

AbstractWe present a compact W‐band mixer‐first receiver consisting of a passive single‐balanced mixer, on‐chip balun, and a variable gain amplifier with 1.9 GHz of bandwidth over a gain range of 36 dB in 65 nm CMOS. The W‐band front‐end has been implemented aiming at a compact frequency‐modulated continuous‐wave (FMCW) radar as a proximity sensor, and a down‐converting chain for a total power radiometer combined with external low noise amplifier. The measured gain of the W‐band receiver is 25 dB, and the receiver achieves 2.57 dBm of IIP3. The integrated receiver consumes 14.56 mA from a supply voltage of 1.2 V, and the core chip occupies only 0.39 mm2.
Quasi‐elliptical band‐pass filters based on compact spiral resonators for C‐band applications
Authors: Cidronali Alessandro , Collodi Giovanni , Maddio Stefano , Pelosi Giuseppe , Selleri Stefano 
Published: February 2019

AbstractThis letter proposes a quasi‐elliptical passband filter in via‐less microstrip technology. The filter is based on the Archimedean spiral, enabling a compact and low‐loss response with sharp transitions to stop bands. The developed filters fabricated on a commercial substrate, present losses below 2 dB within a 3 dB bandwidth of 500 MHz, and isolation exceeding 20 dB. Its extremely compact dimension, about , and the low‐cost of fabrication, permits the integration within compact front‐end PCB.
Investigation of magnetic resonance coupling circuit topologies for wireless power transmission
Authors: Wang Jingchen , Leach Mark , Lim Eng Gee , Wang Zhao , Huang Yi 
Published: February 2019

AbstractMagnetic resonance coupling circuits have four general topologies; however, there is a lack of comprehensive theoretical analysis with experimental verification for each of these topologies regarding their attractiveness for wireless power transfer (WPT). This article provides this for each of the four topologies to fully understand their differences and allow the selection of the most appropriate type based on system requirements. In addition, a problem associated with the resonance coupling method is the phenomenon of frequency splitting, which can lead to a high‐power transfer efficiency but low‐load power at the resonant frequency. Reasons for frequency splitting and methods of circumventing the problem will be illustrated in this article. Of the four topologies, the series‐parallel (SP) (input‐output) circuit configuration is the most efficient for the realization of a WPT system with a large load impedance, in terms of achieving both a high power transfer efficiency and high‐load power.
Compact quad‐band second harmonic antenna based on metamaterial DRA load
Authors: Pedram Kumars , Naderi Mohammad , Jafari Fereshteh Sadat , Zarrabi Ferdows B. 
Published: February 2019

AbstractWe have developed a compact antenna for wireless applications with a new shape and special attribute. It is a dual‐band antenna with a second harmonic characteristic. The antenna has a special shape of the dielectric resonator with metamaterial meandered composite right/left‐hand (CRLH) structure. The Microstrip ring antenna is the right‐hand part. We used the Microstrip meandered line via inductance for the unit cells to achieve a circular polarization and to change the current distribution at the surface of the antenna. We added a parasitic ring to the antenna to achieve the second harmonic resonance. We have connected three unit cells at the 120° angle. This special shape provides the circular polarization and compact size. The antenna has quad‐band at the first harmonic, and the resonances occurred at 2.25 and 2.8 GHz. The second harmonics happened at 4.5 and 5.6 GHz.