bluuebunny/crossref_metadata_2025_split huggingface dataset:
DEVELOPMENT AND VALIDATION OF CLINICALLY RELEVANT ANIMAL MODEL OF MENISCAL DEGENERATION
Authors: D’Arrigo D. , Conte P. , Anzillotti G. , Giancamillo A. d. , Girolamo L. d. , Peretti G. , Crovace A. , Kon E. 
Published: November 2024

IntroductionDegenerative meniscal tears are the most common meniscal lesions, representing huge clinical and socio-economic burdens. Their role in knee osteoarthritis (OA) onset and progression is well established and demonstrated by several retrospective studies. Effective preventive measures and non-surgical treatments for degenerative meniscal lesions are still lacking, also because of the lack of specific and accurate animal models in which test them. Thus, we aim to develop and validate an accurate animal model of meniscus degeneration.MethodThree different surgical techniques to induce medial meniscus degenerative changes in ovine model were performed and compared. A total of 32 sheep (stifle joints) were subjected to either one of the following surgical procedures: a) direct arthroscopic mechanical meniscal injury; b) peripheral devascularization and denervation of medial meniscus; c) full thickness medial femoral condyle cartilage lesion. In all the 3 groups, the contralateral joint served as a control.ResultFrom a visual examination of the knee joint emerged a clear difference between control and operated groups, in the menisci but also in the cartilage, indicating the onset of OA-related cartilage degeneration. The meniscal and cartilaginous lesions were characterized by different severity and location in the different groups. For instance, a direct meniscal injury caused cartilaginous lesions especially in the medial part of the condyles, and the other approaches presented specific signature. Evaluation of scoring scales (e.g. ICRS score) allowed the quantification of the damage and the identification of differences among the four groups.ConclusionWe were effectively able to develop and validate a sheep model of meniscal degeneration which led to the onset of OA. This innovative model will allow to test in a pre-clinical relevant setting innovative approaches to prevent meniscal-related OA.FundingProject PNRR-MAD-2022-12375978 funded by Italian Ministry of Health
EXERKINE FNDC5 PRESERVES BONE ANABOLISM AND COUNTERACTS OESTROGEN DEFICIENCY-INDUCED OSTEOPOROSIS DEVELOPMENT
Authors: Lin Y. , Lian W. , Chen Y. , Wang F. 
Published: November 2024

IntroductionOsteoporosis accounts for a major risk factor of fracture-associated disability or premature death in the elderly. Enhancement of bone anabolism for slowing osteoporosis is highly demanding. Exerkine fibronectin type III domain containing 5 (FNDC5) regulates energy metabolism, inflammation, and aging. This study was aimed to investigate whether Fndc5 signaling in osteoblasts changed estrogen deficiency-mediated bone loss or microarchitecture deterioration.MethodFemale osteoblast-specific Fndc5 transgenic mice (Fndc5Tg), which overexpressed Fndc5 under the control of key osteoblast marker osteocalcin promoter, were given bilateral ovariectomy to induce estrogen deficiency-mediated osteoporosis. Bone mass, microstructures, and biomechanical properties were quantified using μCT imaging and material testing. Dynamic bone formation was traced using fluorescence calcein. Osteogenic differentiation and adipocyte formation of bone-marrow mesenchymal cells were investigated using von Kossa staining and Nile red staining, respectively. Serum osteocalcin, CTX-1 and TRAP5b levels were quantified using designated ELISA kits. Mitochondrial respiration was investigated using Seahorse Extracellular Flux Analyzer.ResultFndc5Tg mice developed relatively higher bone mass and microarchitecture than wild-type mice. Fndc5 overexpression attenuated the losses of bone mineral density and trabecular network, including trabecular volume, thickness, and trabecular number, and improved cortical thickness and porosity in ovariectomized mice. Gain of Fndc5 function preserved biomechanical characteristics (maximum load, breaking force, and energy), serum bone formation marker osteocalcin levels, and bone formation rate, whereas it reduced serum bone resorption makers CTX-1 and TRAP5b levels, osteoclast overburden, and marrow adiposis. In vitro, Fndc5 reversed the estrogen deficiency-mediated mineralized matrix underproduction and adipocyte formation of bone-marrow mesenchymal cells, and inhibited osteoclast formation in osteoporotic bone. Mechanistically, Fndc5 activated AMPK signaling, promoting mitochondrial respiration and ATP production to enhance osteoblastic activity.ConclusionFndc5 signaling exerted bone-protective actions delaying estrogen deficiency-mediated osteoporosis. This study highlighted a new molecular remedial option for osteoporosis development by manipulating Fndc5 functions.
A protocol for research on the use of acupuncture in the management of diabetic peripheral neuropathy in individuals with type 2 diabetes: A systematic review and meta-analysis
Authors: Fan Xuechun , Cao Jingsi , Yan Guanchi , Zhao Yunyun , Wang Ying , Wang Xiuge , Mi Jia 
Published: November 2024

Introduction
Diabetic peripheral neuropathy (DPN), a prevalent complication among individuals diagnosed with type 2 diabetes, has a significant impact on both the well-being of patients and their financial situation. Acupuncture has been employed for thousands of years within China and is regarded as one of the primary characteristic therapies of traditional Chinese medicine. Research has indicated that acupuncture has the potential to enhance microcirculation, decrease the generation of free radicals, and augment nerve conduction velocity. There had been several meta-analyses of acupuncture on DPN. Nevertheless, there has been inadequate attention given to the assessment of blood glucose control, and scores related to quality of life. Hence, we get additional evidence by enhancing the quantity and quality of studies to draw more distinct findings.

Methods
We will conduct a comprehensive search for reports published from the beginning until June 2023 using various databases including Web of Science, Embase, Cochrane Library, PubMed, AMED, Wanfang database, VIP database, China National Knowledge Infrastructure, and Chinese Biomedical Literature database. Only randomized controlled trials will be considered, with no exclusion of quasi-randomized control trials. Articles in both English and Chinese will be taken into account without any limitations on publication dates. The data will be extracted, managed, and analyzed by two researchers working independently. The primary outcomes will include improvement of symptom scores, change of nerve conduction velocity, and quality of life scores. Additional outcomes will encompass blood glucose levels after fasting and 2 hours after eating, levels of glycosylated hemoglobin, and any adverse events associated with acupuncture. We plan to use the RevMan V.5.4 application and the random-effects model for conducting the meta-analysis. The assessment of potential prejudice can be conducted by Cochrane’s ’risk of bias’ 2 (RoB 2) tool. Registration: PROSPERO (registration number: CRD42023425203).

Discussion
Our goal is to perform a meta-analysis that offers an unbiased approach to treating individuals with type 2 DPN. At the same time, it also provides doctors with more choices in the treatment of diabetes peripheral neuropathy.
Examining preventive occupational health and safety management in the Swedish welfare sector–questionnaire development, its validity and reliability, and initial findings on employers’ knowledge
Authors: Akerstrom Magnus , Severin Jonathan , Roczniewska Marta , Jonsdottir Ingibjörg H. , Hadzibajramovic Emina 
Published: November 2024

Introduction
A preventive approach to occupational health and safety management (OHSM) can improve working conditions, but more knowledge is needed on how this should be organised in practice. Here, we describe the development, validity and reliability of a questionnaire used to examine employers’ preventive OHSM within the Swedish welfare sector. Furthermore, employers’ knowledge of preventive OHSM was explored using the survey data.

Material and methods
A questionnaire was developed based on interviews with key actors (n = 7), experts (n = 6) and intended respondents (n = 5). Using the final questionnaire, 197 responses were collected from employer (n = 126) and employee representatives (n = 71) and used to assess the validity and reliability of the questionnaire. Qualitative and quantitative analyses of open-ended and multi-choice items were used to assess the response distribution, content validity and interrater reliability (i.e. employer-employee correspondence from 32 matched pairs from the same workplace). Quantitative and qualitative analyses of survey responses from employer representatives were performed to assess their knowledge of preventive OHSM.

Results
The final questionnaire included 91 items covering employers’ working routines, resources and work environment economics. Qualitative analyses indicated a high content validity in the questionnaire, with a satisfactory response distribution and very small proportion of missing data on individual survey items. Overall, the interrater reliability was high (>60%), but the employer representatives generally gave more examples within different areas of the OHSM compared to the employee representatives. Most of the employer representatives (74%, n = 90) assessed that their organisation had sufficient knowledge regarding preventive OHSM but that the knowledge of work environment economics was lower.

Conclusions
By combining work environment research with questionnaire design research, high validity and reliability was achieved for this questionnaire. Furthermore, the employers generally perceived that they have high knowledge of preventive OHSM but that more knowledge is needed on work environment economics.
Hedonic Responses to Meal Ingestion in Patients With Functional Dyspepsia and Postprandial Symptoms
Authors: Livovsky Dan M. , Barber Claudia , Azpiroz Fernando 
Published: November 2024

INTRODUCTION:
It is not clear whether patients with postprandial symptoms enjoy the meal.

METHODS:
Prospective trial in patients with postprandial distress syndrome and healthy controls, comparing their responses with stepwise ingestion of a comfort meal.

RESULTS:
Healthy individuals (N = 18) enjoyed the meal and experienced digestive well-being. Despite their symptoms, some patients enjoyed the meal as much as healthy individuals (n = 9), while others did not (n = 15), and both groups experienced the same degree of early satiety, fullness, and discomfort.

DISCUSSION:
The hedonic response to meal ingestion is preserved in a proportion of patients with postprandial distress syndrome.
Effectiveness of Ustekinumab for Patients With Moderate-to-Severe Ulcerative Colitis: A Multicenter Real-World Canadian Study
Authors: Solitano Virginia , Narula Neeraj , Ma Christopher , Nanayakkara Akithma , Suarez Kenneth Ernest , Zoughlami Amine , Guizzetti Leonardo , Bessissow Talat , Jairath Vipul 
Published: November 2024

INTRODUCTION:
We aimed to evaluate the real-world effectiveness and safety of ustekinumab for the treatment of both bio-naive and bio-exposed ulcerative colitis patients in a real-world setting.

METHODS:
Retrospective, Canadian multicenter cohort study. Primary outcomes were clinical remission and endoscopic remission. Key secondary outcomes included composite clinical and endoscopic response, safety, and persistence. Univariable logistic regression was conducted to examine associations between baseline factors and 12-month clinical and endoscopic response and remission. The primary focus was on the association between these outcomes and bioexposure status.

RESULTS:
A total of 198 patients were included, of whom 47.5% were female and 85.9% were younger than 65 years. The majority had severe endoscopic activity at baseline (47.5%, n = 87) and were bio-exposed (88.8%, n = 176; 33%, n = 66 previous failure of 3 or more biologics). Patients were followed for a median of 12.8 (interquartile range 8.2–22.1) months after induction. The overall clinical remission rates were 41.3% (69/167), 38.1% (56/147), and 43.6% (58/133) at 3, 6 and 12 months, respectively. Endoscopic remission rates (Mayo endoscopy score = 0) were 8.0% (7/88), 18.7% (23/123), and 12.5% (11/88) at 3, 6, and 12 months, respectively, and consistently higher in bio-naive patients compared with bio-exposed patients through 12 months (P < 0.05 at all time points). Bio-naive patients were more likely to achieve endoscopic remission compared with bio-exposed (hazard ratio = 5.40, 95% confidence interval [CI]: 1.08–26.93). Adverse events were reported in 14.1% (18/198).

DISCUSSION:
In a highly refractory and largely bio-exposed population, a substantial proportion of patients with ulcerative colitis treated with ustekinumab can achieve clinical and endoscopic outcomes of importance after 12 months. Previous biologic exposure is associated with lower rates of endoscopic remission.
Candidate Genetic Loci Modifying the Colorectal Cancer Risk Caused by Lifestyle Risk Factors
Authors: Barot Shabane , Vermani Litika , Blom Johannes , Larsson Susanna , Liljegren Annelie , Lindblom Annika 
Published: November 2024

INTRODUCTION:
65%–70% of colorectal cancer (CRC) cases are considered sporadic; they arise under the influence of environmental factors in individuals lacking a family history of CRC. Low-risk genetic variants are believed to contribute to CRC risk, in tandem with lifestyle factors.

METHODS:
Six hundred sixteen nonfamilial Swedish CRC cases with at least 1 of the following 5 risk factors: smoking, excessive alcohol consumption, physical inactivity, adherence to an unhealthy diet, and excess body weight were included in this study. A control group consisting of 1,642 healthy individuals was used. Cases and controls were genotyped from blood samples at the Centre for Inherited Disease Research at Johns Hopkins University within the Colorectal Transdisciplinary Study research collaboration, using the Illumina Infinium OncoArray-500 K BeadChip. Five separate genome-wide haplotype association analyses were performed, one for each risk factor. Logistic regression models were used to estimate associations between haplotypes (exposure) and CRC (outcome) in cases with lifestyle risk factors vs controls. Haplotypes with an odds ratio >1 were considered candidate risk markers, denoting an area of interest in the genome. A significance threshold of P < 5 × 10−8 was used.

RESULTS:
We found 17 haplotype regions significantly associated with CRC in cases vs controls. Several regions included genes linked to inflammation and tumor promotion.

DISCUSSION:
We concluded that having certain genetic variants was associated with an increased risk of CRC compared with healthy controls among cases with known lifestyle risk factors. The interplay of lifestyle and genetic risk factors calls for further elucidation.
Uji Karakteristik Bioetanol Campuran Kulit Nanas dan Buah Pisang Hutan
Authors: Sulaiman Dady , Huturlukitaningtyas Dhea Fambayun , Sari Ayu Lingga Ratna , Ulva Siti Maria 
Published: November 2024

Menipisnya persediaan energi fosil mendorong penelitian mengembangkan sumber energi alternatif dari sumber daya alam yang bisa diperbarui salah satunya yaitu biomassa. Biomassa yang ingin diteliti oleh peneliti disini berupa bioetanol. Bioetanol adalah cairan biokimia pada proses fermentasi gula dari sumber karbohidrat dengan menggunakan bantuan mikroorganisme dilanjutkan dengan proses destilasi. Penelitian ini menggunakan bahan campuran kulit nanas dan buah pisang hutan dengan harapan kadar bioetanol dapat meningkat. Penelitian ini bertujuan untuk mengetahui proses pembuatan bioetanol campuran kulit nanas dan buah pisang hutan. Jenis penelitian ini adalah penelitian ekperimen dengan metode kuantitatif yang akan menganalisis karakteristik bioetanol dengan variasi komposisi kulit nanas dan buah pisang hutan, variasi A (80% kulit nanas 20% pisang hutan) variasi B (50% kulit nanas 50% pisang hutan) variasi C (20% kulit nanas 80% pisang hutan). Adapun hasil yang diperoleh dari ekperimen ini, variasi A mendapatkan hasil kadar alkohol 60%, 85,75 kadar air, 0,73 gr/ml densitas, 210 J nilai kalor, variasi B mendapatkan hasil kadar alkohol 53%, 86,7% kadar air, 0,71 gr/ml densitas, variasi C mendapatkan hasil kadar alkohol 48%, 85,8% kadar air, 0,74 gr/ml densitas. Variasi A merupakan hasil terbaik yang menghasilkan 210 J, Kadar Alkohol 60%, Densitas 0,73 gr/ml, dan Nilai kadar air terendah sebesar 85,7%.
A decision support system to increase the compliance of diagnostic imaging examinations with imaging guidelines: focused on cerebrovascular diseases
Authors: Moghaddasi Hamid , Rahimi Fatemeh , Seddighi Amir Saied , Akbarpour Leila , Roshanpoor Arash 
Published: November 2024

Abstract

Objectives
Diagnostic imaging decision support (DI-DS) system has emerged as an innovative evidence-based solution to decrease inappropriate diagnostic imaging. The aim of the present study was to design and evaluate a DI-DS system for cerebrovascular diseases.

Methods
The present study was an applied piece of research. First, the conceptual model of the DI-DS system was designed based on its functional and non-functional requirements. Afterwards, to create the system’s knowledge base, cerebrovascular diseases diagnostic imaging algorithms were extracted from the American College of Radiology Appropriateness Criteria (ACR-AC). Subsequently, the system was developed based on the obtained conceptual model and the extracted algorithms. The software was programmed by means of the C#. After debugging the system, it was evaluated regarding its performance and also the users’ satisfaction with it.

Results
Assessing the users’ satisfaction with the system demonstrated that all the evaluation criteria met the acceptable threshold (85 %). The retrospective evaluation of the system’s performance indicated that from among 76 imaging examinations, which had previously been performed for 30 patients, 12 (15.78 %) were deemed inappropriate. And, the system accurately identified all the inappropriate physicians’ decisions. The concurrent evaluation of the system’s performance indicated that the system’s recommendations helped the physicians remove 100 % (4 out of 4) of the inappropriate and 40 % (2 out of 5) of the inconclusive imaging examinations from their initial choices.

Conclusions
A DI-DS system could increase the compliance of the physicians’ decisions with diagnostic imaging guidelines, and also improve treatment outcomes through correct diagnosis and providing timely care.
A Comprehensive Analysis Using Maximum Likelihood Estimation and Artificial Neural Networks for Modeling Arthritic Pain Relief Data
Authors: G S Deepthy , Areekara Sujesh , Sebastian Nicy 
Published: November 2024

Abstract
The primary motivation behind this study is to precisely predicting the behaviour of the distribution by employing neural networks and enhancing its performance through maximum likelihood estimation. The numerical findings were compared to the predictions derived from the multilayer artificial neural network model developed with seven neurons in the hidden layer. The R value was 0.999 and the deviation values were less than 0.045  for the artificial neural network models. Also, the results of a numerical investigation using maximum likelihood estimation agree exactly with those obtained from predictions made using artificial neural networks. The findings of this study reveal that neural networks might be a very promising tool for clinical data analysis.
On translations of certain subsets of uncountable commutative groups
Authors: Kharazishvili Alexander 
Published: November 2024

Abstract
For an uncountable commutative group

(
G
,
+
)

{(G,+)}

and for an infinite cardinal
κ, the notions of a

(
G
,
κ
)

{(G,\kappa)}

-thick set in G and of a

(
G
,
κ
)

{(G,\kappa)}

-thin set in G
are introduced and examined. It is shown that some statements about these sets are closely
connected with the existence of absolutely
nonmeasurable subsets of

(
G
,
+
)

{(G,+)}

and with additional set-theoretical axioms.
Data Transactions in China: Legislation, Promotion, and Regulation
Authors: Yang Li , Hu Jieren 
Published: November 2024

Abstract
The difficulty of deepening the promotion of “factor-level” data circulation and utilization lies in the feasibility of the legislation of data transaction. This involves meta-issues such as the definition of data ownership, the positioning of data assets, and privacy protection systems. From the perspective of inclination, i.e., focusing on promotion while giving consideration to regulation, the legislation of data transaction in China is established on the theoretical basis of an “incomplete contract”. Such perspective identifies different areas that can be the subject of regulation, ranging from data rights, behavior control to the separation of personal data and information. On this basis, the driving mode of the legislation of data circulation and utilization is not the simple and direct data transaction with market-oriented mechanism, but the exploration of data licensing under government authorized operation beforehand in China. Such approaches should be considered during the licensing of public data, and the government’s right to obtain necessary compensations for the costs of data processing should be reserved. What’s more, legislative clauses of data security are essential in transactions, but compliance and conditional liability exemptions can be adopted to safeguard the realization of the goals of data transaction to a great extent.
Tamarind seed powder as filler in polypropylene and its impact on the mechanical and biodegradability of the composites
Authors: Mohanty Sucheta , Shukla Shubham , Senapati Debasis , Sahoo Gourav , Sahoo Debanshu , Mohanty Shyama Prasad 
Published: November 2024

Abstract
In the present work, agro-industrial waste has been blended with plastic waste to fabricate value added product and also reduce the environmental pollution. Tamarind seed powder (TSP) has been melt mixed with polypropylene (PP) waste in various proportions to prepare composite sheets. The composite specimens have been evaluated for their tensile strength, impact strength and hardness. In composites, highest tensile strength of 20.6 MPa and Young’s modulus of 953.3 MPa have been achieved with 30 wt% and 40 wt% of TSP, respectively. Composites have lower strength but better hardness than PP. Maximum hardness of 69.7 HRL has been obtained in composites with 40 wt% TSP. Poor interfacial bonding as observed in fractured specimens is responsible for lower strength in composites. However, the composite specimen has better thermal stability than PP. Weight loss has been observed in composite specimens with higher loading of TSP due to degradation of TSP upon subjecting the specimens to biodegradability tests. Based on the results obtained in the study, 40 wt% of TSP in the composites conveys suitable mechanical and thermal properties.
Randomized vector algorithm with iterative refinement for solving boundary integral equations
Authors: Sabelfeld Karl K. , Agarkov Georgy 
Published: November 2024

Abstract
This study is a follow-up of two our papers (Appl. Math. Lett. 126 (2022) and MCMA 29:4 (2023)),
where we developed a vector randomized algorithms with iterative refinement for large system of linear algebraic equations.
We focus in this paper on the application of the vector randomized iterative refinement algorithm
to boundary integral equations that solve interior Dirichlet and exterior Neumann boundary value problems for
2D Laplace equation.