bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single nucleotide polymorphisms in transcription factor genes associated with susceptibility to oral cancer
Authors: Damani Shah Hetal , Saranath Dhananjaya , Pradhan Sultan 
Published: August 2019

AbstractOral cancer is a major public health concern in the Asian countries predominated by India which accounts for 33.81% of the annual global oral cancer burden. The well‐established high‐risk factors associated with oral cancer include tobacco, areca nut, alcohol consumption, and high‐risk human papilloma virus types 16/18. Additionally, in the past two decades, the critical role of the genomic constitution of individuals in oral cancer susceptibility has emerged. Accumulating evidence indicates the association of several single nucleotide polymorphisms (SNPs) with oral cancer risk. Thus in the current study, we assessed the association of thirteen SNPs in seven transcription factor genes along with HBB (a control SNP) to identify high‐risk genotypes associated with increased oral cancer risk in an Indian cohort of tobacco habitués. Fourteen SNPs were investigated in 500 patients with oral cancer and 500 clinically healthy long‐term tobacco users as controls of Indian ethnicity. Allelic discrimination real‐time polymerase chain reaction was the method of choice for genotyping the samples. Logistic regression analysis was performed and the association of SNPs with oral cancer risk was estimated using odds ratio (OR) and 95% confidence interval (CI). We observed five SNPs—rs2051526 (ETV6), rs6021247 (NFATC2), rs3757769 (SND1), rs7085532 (TCF7L2), and rs7778413 (SND1) indicating increased oral cancer risk with OR ranging from 1.61 to 34.60. Further, as a proof of concept, the coinheritance of high‐risk genotypes in rs6021247 (NFATC2) GG (OR, 2.77; CI, 2.09‐3.69) and rs7778413 (SND1) CC (OR, 34.60; CI, 17.32‐69.13) reflected further increase in the risk with OR‐49.94 (CI, 16.25‐153.48). The present study indicates the association of transcription factor SNPs with increased oral cancer risk constituting “predictive biomarkers” in oral cancers.
Comparative study of μ‐stat methanol feeding control in fed‐batch fermentation of Pichia pastoris producing HBsAg: an open‐loop control versus recurrent artificial neural network‐based feedback control
Authors: Beiroti Ahmad , Hosseini Seyed Nezamedin , Aghasadeghi Mohammad Reza , Norouzian Dariush 
Published: August 2019

AbstractBACKGROUNDIn recent decades, artificial neural network (ANN) has been shown to be a robust and promising tool in monitoring and controlling bioprocess systems. In a previous study, the authors designed a highly accurate and precise recurrent neural network (RNN) for predicting the biomass amount of recombinant Pichia pastoris Mut+ producing intracellular hepatitis B surface antigen (HBsAg) during fed‐batch methanol fermentation. In the current work, the aim was to compare the production efficiency of HBsAg between conventional predefined μ‐stat methanol feeding control (open‐loop control) – already established in large‐scale production – and methanol feeding based on a μ‐stat feedback control system using RNN. For this purpose, for each methanol feeding strategy, bench‐scale, fed‐batch fermentation processes were carried out twice.RESULTSAccording to the results, in contrast to the established μ‐stat predefined feeding strategy (open‐loop), the deviation of specific growth rate and biomass in μ‐stat feedback control based on RNN was negligible. Also, in the suggested methanol feeding control strategy, the HBsAg titer, specific productivity and yield between the performed fed‐batch fermentations – unlike the conventional method – were approximately identical, with average values of 110.8 μg mL−1, 1.52 μg g−1 dry biomass and 0.34 μg g−1 MeOH respectively.CONCLUSIONComparing the biomass growth pattern and HBsAg production efficiency with the conventional μ‐stat predefined feeding in open‐loop control, the new proposed feeding control system illustrated significantly high process efficiency. This reliable control system based on ANN can have many applications in the biopharmaceutical industry for the control of process key parameters as well as for enhancing process efficiency. © 2019 Society of Chemical Industry
Twenty‐year results after connective tissue grafts and guided tissue regeneration for root coverage
Authors: Petsos Hari , Eickholz Peter , Ratka‐Krüger Petra , Neukranz Erik , Nickles Katrin 
Published: August 2019

AbstractBackgroundEvaluation of clinical long‐term results 20 years after connective tissue grafting (CTG) or guided tissue regeneration (GTR) using bioabsorbable barriers for root coverage therapy.MethodsInitially, 15 patients with 38 Miller Class I and II recession defects underwent CTG or GTR according to random assignment. At baseline, 3, 120 ± 12, and 240 ± 12 months after surgery, data on probing depth, clinical attachment level, recession depth and width, amount of keratinized tissue, and bleeding on probing were obtained. Additionally, patients’ smoking habits and participation in supportive periodontal therapy were investigated.ResultsEight patients contributing 23 recessions were available at the 240 ± 12 months follow‐up. Three and 120 ± 12 months after therapy with CTG, significantly better root coverage was observed compared with baseline (3 months: 3.01 ± 1.74 mm; P = 0.003; 120 ± 12 months: 2.11 ± 1.86 mm; P < 0.024). GTR resulted in significantly better root coverage compared with baseline after 3 months (2.25 ± 1.89 mm; P < 0.012). Although there were no significant changes in the recession depth between 3 and 240 ± 12 months in both groups (CTG: P = 0.097; GTR: P = 0.190), 1.57 ± 2.12 mm (CTG) and 1.19 ± 2.31 mm (GTR) of the achieved coverage after 3 months were lost. CTG showed significantly better relative root coverage percentage than GTR after 3 (P = 0.026) and 120 (P = 0.038) months. This study failed to detect a significant difference in the stability of root coverage after 240 ± 12 months between CTG and GTR (P = 0.448) and patients’ assessments of their treatment outcomes (P = 0.503).ConclusionLong‐term stability of root coverage and patient‐perceived esthetic outcomes failed to show significant differences between CTG and GTR at 20 years post‐surgery.
Post‐surgical flap placement following osseous surgery: A short‐term clinical evaluation
Authors: Penner Jeffrey K. , Deas David E. , Mills Michael P. , Hanlon John , Gelfond Jonathan , Hernandez Brian , Mealey Brian L. 
Published: August 2019

AbstractBackgroundThe purpose of this study is to examine the relationship between immediate post‐surgical flap position and subsequent probing depth measurements following osseous surgery.MethodsTwenty‐four patients treatment planned for osseous surgery after completion of initial therapy and re‐evaluation were enrolled. Pressure molded stents were fabricated to serve as a reference for probing depth and relative attachment level measurements prior to surgery. After osseous recontouring was completed, flaps were sutured and compressed, and bone sounding measurements were made as designated by the stent. Patients returned at 3‐ and 6 months for repeat measurements of probing depth and attachment level.ResultsTwenty‐four patients completed surgical treatment and follow‐up measurements with a total of 402 treated sites. A statistically significant moderate correlation between immediate post‐surgical bone sounding measurements and subsequent probing depth was found at 6 months (R = 0.56, P < 0.001). There was no significant difference between this correlation at 3 and 6 months. The probability of having 6 month probing depth ≤3 mm was 93.5% when the surgical flap was placed within 3 mm of the alveolar crest (286/306 sites) as opposed to 50% when the surgical flap was >3 mm away from the alveolar crest (48/96 sites). Interproximal sites were significantly more likely (P < 0.01) to have probing depths > 3 mm at 3 and 6 months.ConclusionsResults suggest a statistically significant relationship between immediate post‐surgical flap placement and subsequent probing depths. Positioning the surgical flap more closely to the alveolar crest when performing osseous surgery resulted in shallower probing depths at 3 and 6 months.
Molecularly imprinted polymer coating on metal‐organic frameworks for solid‐phase extraction of fluoroquinolones from water
Authors: Zhao Shanwen , Sun Zhian , Liu Huachun , Zhou Yanqiang , Li Jianmin , Wang Xiaoxiao , Gong Bolin 
Published: August 2019

AbstractIn this work, a novel surface molecularly imprinted polymer with high adsorption capacity, high adsorption rate, and high selectivity for fluoroquinolones was prepared on the surface of UiO‐66‐NH2, which is a kind of metal‐organic framework. The surface morphology and adsorption properties of this molecularly imprinted polymer were investigated. The maximum adsorption capacity was 99.19 mg/g, and adsorption equilibrium was achieved within 65 s. Combined with reversed‐phase high‐performance liquid chromatography, the molecularly imprinted polymer was used to selectively enrich, separate and analyze fluoroquinolones present in lake water. The results showed that the recoveries of the four fluoroquinolones were 92.6–100.5%, and the relative standard deviations were 2.9–6.4% (n = 3). The novel molecularly imprinted polymer is an excellent adsorbent and has broad application prospects in the enrichment and separation of trace analytes in complex samples.
In the wake of a major hurricane: Differential effects on early vs. late successional seagrass species
Authors: Congdon Victoria M. , Bonsell Christina , Cuddy Meaghan R. , Dunton Kenneth H. 
Published: August 2019

AbstractAt least 18 major storms have struck the Gulf of Mexico and Caribbean in the past 50 yr including Hurricane Harvey, a Category 4 storm that passed over extensive seagrass beds in the western Gulf of Mexico and became the second‐most expensive U.S. hurricane. We sought to identify the effects of an extreme hurricane on sediment physicochemical characteristics and seagrass species with contrasting life histories and morphologies. Surprisingly, Harvey's intense wind speeds resulted in decreases in blade length, vegetative cover, and greater overall loss of Thalassia, a persistent climax species relative to Halodule, a prolific pioneer species. Sediment ammonium and grain size changed, but not organic carbon. Our results indicate that effects of wind intensity are not only restricted to the differential impacts on seagrasses, but on the physicochemical characteristics of the sediments. These changes, coupled with the slow colonization abilities of Thalassia, may prolong recovery of disturbed seagrass meadows.
Self‐Healing Properties of PDMS Elastomers via Guanine and Cytosine Base Pairs
Authors: Chen Ling , Peng Hao , Wei Yingying , Wang Xiaoyu , Jin Yajing , Liu Houtao , Jiang Yong 
Published: August 2019

AbstractOn account of the combination of base pairs interaction, DNA can realize, unlock, and repair. By introducing commercially available guanine and cytosine into poly(dimethyl siloxane) (PDMS), the self‐healing supramolecular polymer can be achieved, which will be of great interest. Herein, a kind of base pairs functionalized PDMS‐based elastomer is successfully developed and demonstrates great elasticity, tensile strength, and self‐healing abilities, such as GC‐PDMS‐10k, which can be stretched more than triple the original length and achieve 75% self‐healing efficiency. Changing the molecular weights of PDMS or the base pairs offers the desired mechanical properties. Importantly, the synthesis approach is simple enough with no external solvent.
Comparison of specific endophytic bacterial communities in different developmental stages of Passiflora incarnata using culture‐dependent and culture‐independent analysis
Authors: Goulart Marcela C. , Cueva‐Yesquén Luis G. , Hidalgo Martinez Kelly J. , Attili‐Angelis Derlene , Fantinatti‐Garboggini Fabiana 
Published: August 2019

AbstractPlants and endophytic microorganisms have coevolved unique relationships over many generations. Plants show a specific physiological status in each developmental stage, which may determine the occurrence and dominance of specific endophytic populations with a predetermined ecological role. This study aimed to compare and determine the structure and composition of cultivable and uncultivable bacterial endophytic communities in vegetative and reproductive stages (RS) of Passiflora incarnata. To that end, the endophytic communities were assessed by plating and Illumina‐based 16S rRNA gene amplicon sequencing. Two hundred and four cultivable bacterial strains were successfully isolated. From the plant’s RS, the isolated strains were identified mainly as belonging to the genera Sphingomonas, Curtobacterium, and Methylobacterium, whereas Bacillus was the dominant genus isolated from the vegetative stage (VS). From a total of 133,399 sequences obtained from Illumina‐based sequencing, a subset of 25,092 was classified in operational taxonomy units (OTUs). Four hundred and sixteen OTUs were obtained from the VS and 66 from the RS. In the VS, the most abundant families were Pseudoalteromonadaceae and Alicyclobacillaceae, while in the RS, Enterobacteriaceae and Bacillaceae were the most abundant families. The exclusive abundance of specific bacterial populations for each developmental stage suggests that plants may modulate bacterial endophytic community structure in response to different physiological statuses occurring at the different plant developmental stages.
Postnatal clinical phenotype of five patients with Pallister–Killian Syndrome (tetrasomy 12p): Interest of array CGH for diagnosis and review of the literature
Authors: Alqahtani Amerh Salem , Putoux Audrey , Bonnet Dupeyron Marie Noelle , Carneiro Maryline , Lion‐Francois Laurence , Rossi Massimiliano , Tevissen Hélène , Schluth Bolard Caroline , Labalme Audrey , Lesca Gaetan , Till Marianne , Edery Patrick , Sanlaville Damien 
Published: August 2019

AbstractBackgroundPallister–Killian syndrome (PKS) is a rare sporadic disorder caused by tetrasomy of the short arm of chromosome 12. The main clinical manifestations are global developmental delay, intellectual disability, epilepsy, dysmorphic features, hypopigmented and/or hyperpigmented lesions, and multiple congenital anomalies. PKS is associated with tissue mosaicism, which is difficult to diagnose through peripheral blood sample by conventional cytogenetic methods and fluorescence in situ hybridization.MethodsHere, we report five patients with PKS. We delineate their clinical phenotypes and we compare them with previously published cases. We used array Comparative Genomic Hybridization (aCGH) with DNA extracted from peripheral blood samples. The five patients have also been tested by conventional cytogenetics techniques.ResultsFour out of five patients showed tetrasomy 12p by aCGH. Three of the four patients have typical i(12p) and one of the four demonstrated atypical tetrasomy 12p. The percentage of mosaicism was as low as 20%. Our cohort exhibited the typical PKS phenotypes.ConclusionOur results demonstrate the efficacy of aCGH for the diagnosis of PKS from DNA extracted from lymphocytes. Thus, for patients suspected of PKS, we recommend performing aCGH on lymphocytes at an early age before proceeding to skin biopsy. aCGH on peripheral blood samples is sensitive in detecting low level of mosaicism and it is less invasive method than skin biopsy. We reviewed also the literature concerning the previously published PKS patients diagnosed by aCGH.
A start codon mutation of the TSPAN12 gene in Chinese families causes clinical heterogeneous familial exudative vitreoretinopathy
Authors: Li Wei , Wang Ziwei , Sun Yan , Wang Zhuoshi , Bai Jinyue , Xing Bo , Sun Xiao , Wang Lusheng , Li Jiankang , He Wei 
Published: August 2019

AbstractBackgroundFamilial exudative vitreoretinopathy (FEVR) is a severe clinically and genetically heterogeneous retinal disorder characterized with failure of vascular development of the peripheral retina. The symptoms of FEVR vary widely among patients in the same family, and even between the two eyes of a given patient. The purpose of this study was to investigate the molecular mechanisms by which the start codon mutation of the TSPAN12 causes difference in clinical manifestations between individuals in the same family.MethodsNext‐generation sequencing (NGS)‐based target capture sequencing was performed in proband with a diagnosis of FEVR and their normal visual acuity family members. Cosegregation analysis of the candidate causative variant was performed in additional family members by using Sanger sequencing. Complete fundus examination, fundus fluorescein angiography (FFA), and family history collection were performed in all family members. Potential candidate causative variants were verified with reference to guidelines and standards from the American College of Medical Genetics and Genomics.ResultsWe identified a novel heterozygous missense mutation (c.1A>G, p.M1V) localized in the start codon of the TSPAN12 and was detected as a potentially disease‐causing variant for the proband. Retrospective analysis of clinical data, fundus examination, and FFA showed that the mutant carrier presented peripheral retinal vascular anomalies in early stages, and visual acuity did not show significant effects. However, the proband who carried this mutation and his cousin showed typical high‐stage FEVR fundus changes coupled with a sharp decline in vision.ConclusionsWe report a novel start codon mutation (c.1A>G, p.M1V) in the TSPAN12 that causes clinically heterogeneous manifestations. Our results expand the mutation spectrums of TSPAN12, and will be valuable for disease diagnosis, prognosis, genetic counseling, and enriching our understanding of the role of the tetraspanin‐12 protein in the pathogenesis of FEVR.
Improved FBMC/OQAM uplink performance using an antenna array in a highly frequency selective fading channel
Authors: Kim Yekaterina , Ahn Heungseop , Kim Daejin , Choi Seungwon 
Published: August 2019

AbstractFilter bank multicarrier with offset quadrature amplitude modulation (FBMC/OQAM) is an attractive alternative to orthogonal frequency division multiplexing, as it yields a higher spectral efficiency and robustness to frequency dispersion; however, FBMC/OQAM suffers from multipath interference owing to the absence of the cyclic prefix, especially in the signal environments of widely spread multipaths. Although conventional techniques such as multiple‐input multiple‐output along with equalization can combat multipath fading, these techniques do not effectively resolve the multipath interference unless each subcarrier channel can be assumed to be of a flat fading. This article presents a systematic procedure of applying a receive antenna array to FBMC/OQAM systems that removes the multipath interferences using a nicely‐shaped beam pattern for each of the multipaths associated with a severe frequency selectivity. The performance of the proposed array system has been demonstrated in terms of its bit error rate in the adverse multipath‐spread environment of the Extended Typical Urban model of the 3rd Generation Partnership Project, in which each subcarrier channel suffers from a severe frequency‐selective fading. Using various computer simulations, we found that the proposed array system can completely resolve multipath interferences with an array of N = 10, 16, and 22 when Δθ = 15°, 10°, and 5°, respectively, where N and Δθ denote the number of antennas in each subarray and the interpath direction of arrival difference, respectively. In addition, the proposed array system also provides a signal‐to‐noise ratio gain that is proportional to N, once the multipath interferences are successfully resolved.
Natively fat‐suppressed 5D whole‐heart MRI with a radial free‐running fast‐interrupted steady‐state (FISS) sequence at 1.5T and 3T
Authors: Bastiaansen Jessica A.M. , Piccini Davide , Di Sopra Lorenzo , Roy Christopher W. , Heerfordt John , Edelman Robert R. , Koktzoglou Ioannis , Yerly Jérôme , Stuber Matthias 
Published: August 2019

PurposeTo implement, optimize, and test fast interrupted steady‐state (FISS) for natively fat‐suppressed free‐running 5D whole‐heart MRI at 1.5 tesla (T) and 3T.MethodsFISS was implemented for fully self‐gated free‐running cardiac‐ and respiratory‐motion‐resolved radial imaging of the heart at 1.5T and 3T. Numerical simulations and phantom scans were performed to compare fat suppression characteristics and to determine parameter ranges (number of readouts [NR] per FISS module and TR) for effective fat suppression. Subsequently, free‐running FISS data were collected in 10 healthy volunteers and images were reconstructed with compressed sensing. All acquisitions were compared with a continuous balanced steady‐state free precession version of the same sequence, and both fat suppression and scan times were analyzed.ResultsSimulations demonstrate a variable width and location of suppression bands in FISS that were dependent on TR and NR. For a fat suppression bandwidth of 100 Hz and NR ≤ 8, simulations demonstrated that a TR between 2.2 ms and 3.0 ms is required at 1.5T, whereas a range of 3.0 ms to 3.5 ms applies at 3T. Fat signal increases with NR. These findings were corroborated in phantom experiments. In volunteers, fat SNR was significantly decreased using FISS compared with balanced steady‐state free precession (P < 0.05) at both field strengths. After protocol optimization, high‐resolution (1.1 mm3) 5D whole‐heart free‐running FISS can be performed with effective fat suppression in under 8 min at 1.5T and 3T at a modest scan time increase compared to balanced steady‐state free precession.ConclusionAn optimal FISS parameter range was determined enabling natively fat‐suppressed 5D whole‐heart free‐running MRI with a single continuous scan at 1.5T and 3T, demonstrating potential for cardiac imaging and noncontrast angiography.
Prognostic value of needle electromyography in traumatic brachial plexus injury
Authors: Impastato David M. , Impastato Katherine A. , Dabestani Parinaz , Ko Jason H. , Bunnell Aaron E. 
Published: August 2019

AbstractIntroductionIn this study we aimed to determine whether needle electromyographic assessment of voluntary motor unit recruitment in traumatic brachial plexus injuries could predict spontaneous motor recovery.MethodsA retrospective study was performed on patients with brachial plexus injury affecting deltoid, supraspinatus/infraspinatus, and biceps brachii. The outcome measure was strength on manual muscle testing at least 1 year after injury. Good outcome was considered strength >3/5 on the Medical Research Council (MRC) scale.ResultsNo muscles with no recruitment (n = 27) at 1‐9 months improved to MRC 4/5 strength at a mean of 2.0 years postinjury. Twenty‐five percent of muscles with discrete or severely reduced recruitment (n = 8) regained strength to >3/5 at a mean of 1.4 years postinjury (P = .047).DiscussionAbsent voluntary motor unit potential recruitment at 1‐9 months predicted poor prognosis for spontaneous recovery. A high percentage of patients with discrete recruitment did not improve to >3/5 strength. These patients should be considered for early nerve transfer surgery.