bluuebunny/crossref_metadata_2025_split huggingface dataset:
MENISCAL RECONSTRUCTION WITH SEMITENDINOSUS TENDON AUTOGRAFT COMBINED TO COLLAGEN MEMBRANE: TECHNIQUE NOTE AND LITERATURE REVIEW
Authors: F Kupczik , MEG Schiavon , B Sbrissia , MD Brunet 
Published: November 2024

Objectives The authors describe a technique for meniscal reconstruction using an autologous semitendinosus graft combined to collagen membrane to treat a 39 yrs-old male patient with post-medial meniscectomy syndrome and show its results. Methods The tendon was duplicated and its ends were sutured with high-strength sutures and attached to an adjustable button on each side. The collagen membrane was sutured circumferentially to the graft and bone marrow aspirated was injected under the membrane. By arthroscopy, bone tunnels were drilled at the insertion of the meniscal roots with a retrograde drill. The graft was inserted in the meniscal position, fixed into root tunnels by the adjustable buttons and sutured to the joint capsule using inside-out and outside-in sutures. Results The patient was evaluated at 3, 6 and 12 months postoperatively and showed good clinical and radiological evolution, being asymptomatic at final evaluation. At MRI, there were a meniscal integration of the graft and no degenerative changes. Conclusion The described technique showed to be reproducible and an alternative to treat symptomatic patients undergoing previous meniscectomy. Besides, it may be performed along concomitant procedures like osteotomy, ligament reconstruction and chondral repair.
FIXATION OF OSTEOCHONDRAL PATELLAR FRACTURE BY TRANSOSSEOUS SUTURE: REPORT OF TWO CASES
Authors: Brunet MD , Kupczik F , Schiavon MEG , Sbrissia B , Nakamura EK , Medeiros MP 
Published: November 2024

Objectives The article aims to explore two cases of osteochondral patellar fracture after acute dislocation, emphasizing the importance of appropriate treatment to prevent early progression to patellofemoral osteoarthritis. The study describes a surgical approach involving transosseous suture fixation and fibrin glue adjuvant, presenting it as a potential alternative over existing techniques. Methods Two patients (a 38 years-old male, and a 12 years-old female) with patellar osteochondral fracture due acute patellar dislocation were underwent surgical intervention. The procedure was performed by medial parapatellar arthrotomy and a transosseous suture fixation by high-strength sutures in the male and absorbable sutures in the female, plus use of fibrin glue as an adjuvant in both. Additionally, reconstruction of the medial patellofemoral ligament (MPFL) with the medial third of the patellar tendon was performed in the male and MPFL and medial patellar tibial ligament using hamstrings in the female. Results Postoperative follow-up revealed good clinical and radiographic outcomes. The patients exhibited complete symptom resolution, full ROM, patellar stability and completely cartilage healing on MRI at 3 and 9 months, respectively. The technique showed potential advantages, including simple reproducibility, low cost, and minimal postoperative complications. Conclusion Fixation of osteochondral patellar fracture by transosseous sutures combined with fibrin glue emerges as a reproducible, cost-effective technique with excellent clinical and radiological outcomes. The method displayed advantages over traditional implants, offering a potential alternative for surgeons. Despite the positive results in these cases, the limited literature calls for further research to confirm the technique superiority compared to isolated sutures and other available methods.
Effectiveness of Treatment with Stem Cells in Injuries of the Anterior Cruciate Ligament: A Systematic Review
Authors: Buscariolo FF , Gholmia C , Milani A 
Published: November 2024

Objectives Due to the lack of consensus, the objective of this systematic review was to describe the results in the literature with greater evidence on the effectiveness of stem cell (SC) treatment in ACL injuries, understanding their indications and updates on recommendation level. Methods A systematic review was conducted in accordance with PRISMA guidelines. Two reviewers independently carried out a search with the same descriptors in the MEDLINE, EMBASE and Web of Science databases, for studies published up to 2023. The following descriptors were used: Stem cell; knee injuries; Anterior Cruciate Ligament; Treatment. The following filters were used to reach the expected final result: “Randomized Controlled Trial; “Randomized Clinical Trial”; “Meta-Analysis”; “Systematic Reviews”; “Pragmatic Clinical Trial”. Results A total of 11 studies met all inclusion/exclusion criteria and were included in this review. There was a very strong correlation between the searches of the two researchers (k=0.841). In the included clinical trials, patients were evaluated primarily based on patient-reported outcome scores, and validated instruments. Clinical trials demonstrated results, but not significantly, post-injection in patients who received SC compared to patients who received hyaluronic acid (HA). There were no significant differences in post-injection outcome scores when comparing patients with and without SC use. Conclusion The clinical application of stem cells for the treatment of ACL injuries may be a good adjunctive treatment option. However, published clinical studies to date have not identified a significant difference, even with apparent clinical improvement.
Epidemiological Analysis of Meniscal Repair in the Brazilian Unified Health System
Authors: Faria JLR , Mello CL , Vaqueiro ACV , Mozella AP , Salim R , Guimaraes JAM 
Published: November 2024

Objectives Analyze data related to the surgical treatment of meniscal lesions in Brazil's public system, observing changes and trends during the studied period (2008 – 2021), and to make future projections. Methods The study was conducted using the DATASUS database (Unified Health System – Ministry of Health, Brazil) to gather national data on the number of meniscectomies and meniscal sutures performed between 2008 and 2021, and the results obtained were stratified by region of the country. Future projections until 2050 were made using least squares regression. Results In Brazil, a total of 91,067 meniscal procedures were performed in the Unified Health System (SUS) from 2008 to 2021, of which 72,452 (80%) were partial or total meniscectomies and 18,615 (20%) were meniscal repairs. In 2019, there was an increase in both the total number of procedures and the number of meniscal repairs. However, the proportion remained the same as in 2008 (77% Meniscectomy / 23% Repair). There was a decrease in the number of procedures performed in 2020 and 2021, the period related to the COVID-19 pandemic, but with a higher proportion of meniscectomies performed (82% and 79%, respectively). The national trend indicates a linear increase in the number of procedures, maintaining a higher proportion of meniscectomies over meniscal repair. Conclusion Meniscal repairs show a trend towards an increase in their incidence in the Brazilian Unified Health System, which is compatible with similar studies conducted in other regions of the world. However, their percentage proportion compared to meniscectomy did not show a significant increase within the public health system.
Institutional experience, formal institutional quality, and firm performance: An analysis of firms from the European Union
Authors: Oliveira Alexandre , Carvalho Fernando , Reis Nuno Rosa 
Published: November 2024

Firms’ success depends on their ability to deal with formal institutional quality. Specifically, firms exposed to a diversified set of institutional profiles can achieve institutional competitive advantage that provides firms with diverse knowledge and broader learning opportunities. While prior studies argued that being exposed to a diversified set of institutional profiles negatively influences firms because institutional knowledge can only be replicated in similar institutional profiles, they missed the point of learning from diversity. The purpose of this study is to further understand how firms’ institutional experience moderates the relationship between formal institutional quality and firms’ performance. We argue that firms with higher and more diverse institutional experience will adapt more efficiently to formal institutional quality, thus improving firms’ performance. We test the hypotheses on 4,011 publicly traded firms from the European Union between 2010 and 2021, and our results show that firms that develop higher institutional experience are better able to efficiently adapt to and leverage formal institutional quality and achieve higher firm performance. We contribute to the ongoing discussion on how formal institutions influence firms’ performance by acknowledging the importance of developing a diversified institutional experience. JEL CLASSIFICATION: F23
Futility of dialysis in patients with tumor lysis syndrome in advanced hematological malignancy: A case series
Authors: Golbus Ashley L , Teruel Benjamin , McMahon Blaithin A 
Published: November 2024

Introduction: Type-B lactic acidosis and tumor lysis syndrome (TLS) can occur in patients with advanced hematological malignancies. In these patients, TLS can lead to an acute kidney injury (AKI) as hyperuricemia and hyperphosphatemia cause crystal nephropathy. Patients with this presentation are frequently started on dialysis. However, it is rare for patients to present with the constellation of hematological malignancy, type-B lactic acidosis, TLS, and AKI, and there is a lack of data on dialysis outcomes in these complex patients. Methods: We performed a single-center retrospective study at the Medical University of South Carolina, using the TriNetX search to identify adult patients presenting with hematological malignancy, type-B lactic acidosis, TLS, and AKI. After applying exclusion criteria, we identified 10 patients. Results: Nine patients had a stage 3 AKI and one patient had a stage 2 AKI. Dialysis was initiated in 70% of patients. The median time from TLS diagnosis to initiation of dialysis was 1 day and the median duration of dialysis was 2 days. Death occurred in 100% of patients within 12 months. The median time from onset of TLS to death was 4.5 days. There was no statistically significant difference in time to death between the dialysis and no dialysis groups ( p = 0.373). Discussion: The mortality rate and short time to death despite treatment optimization suggest that in patients with an underlying hematological malignancy, presentation with the constellation of type-B lactic acidosis, TLS, and AKI is an indicator of poor prognosis. Larger, well-designed studies are needed to determine the utility of dialysis in these patients.
Clinical impact of [18F]FDG-PET/CT in ARI0002h treatment, a CAR-T against BCMA for relapsed/refractory multiple myeloma
Authors: Zugasti Inés , Tormo-Ratera Marta , Oliver-Caldés Aina , Soler-Perromat Juan Carlos , González-Calle Verónica , Moreno David F. , Cabañas Valentín , López-Muñoz Nieves , Bartolomé-Solanas Álvaro , Español-Rego Marta , Reguera-Ortega Juan Luis , Rosiñol Laura , López-Corral Lucía , Tovar Natalia , Rodríguez-Lobato Luis Gerardo , Alvarez Perez Rosa Maria , Varea Sara , Olesti Eulàlia , Gomez-Grande Adolfo , Frutos Laura , Tamayo Pilar , Juan Manel , Moraleda José M. , Urbano-Ispizua Álvaro , González-Navarro E. Azucena , Martínez-López Joaquín , Mateos Maria-Victoria , Tomás Xavier , Setoain Xavier , Fernández de Larrea Carlos 
Published: November 2024

Abstract
Multiple myeloma (MM) remains incurable, with poor outcomes in heavily pretreated patients. Chimeric antigen receptor (CAR) T-cell therapy has emerged as a promising treatment; however, outcomes after such therapy in patients with soft-tissue plasmacytomas and other bone lesions remain poorly understood. This study included 63 patients with relapsed/refractory MM treated either in the CARTBCMA-HCB-01 clinical trial (ARI0002h; academic B-cell maturation antigen [BCMA]–targeted CAR T-cell therapy) or in compassionate use. The aim was to evaluate the impact of soft-tissue involvement (extramedullary [EMD] and paraskeletal [PS] plasmacytomas) in response, survival and safety. Baseline [18F]fluorodeoxyglucose (FDG)–positron emission tomography (PET)/computed tomography (CT) from 5 participating centers were reviewed centrally. Of 63 patients, 52.4% presented plasmacytomas at the time of inclusion (21 PS, exclusively; and 12 EMD). Per responses, there were no significant differences between patients with and without plasmacytomas. A correlation was present between International Myeloma Working Group responses and those obtained by [18F]FDG-PET/CT at day 100 (Bologna criteria). No differences were observed in progression-free survival (PFS) or overall survival (OS) between patients with or without plasmacytomas. However, both PFS and OS were significantly shorter in patients with EMD. Interestingly, [18F]FDG-PET/CT response assessed on day 100, in accordance with the Bologna criteria, was predictive of survival outcomes. A metabolic tumor volume of ≥25 cm3 at baseline [18F]FDG-PET/CT was associated with earlier disease progression and shorter OS. These results highlight the importance of EMD evaluation by [18F]FDG-PET/CT before and after CAR T-cell infusion. This trial was registered at www.ClinicalTrials.gov as #NCT04309981; and EudraCT, 2019-001472-11.
Purified α-Amylase from Bacillus cereus exhibits antibiofilm and antiquorum sensing activities against uropathogenic Escherichia coli, Downregulating fimH, and papC virulence genes: implications for urinary tract infections
Authors: Abo-Kamar Amal M. , Mustafa Abd-El-Rahman A. , Al-Madboly Lamiaa A. 
Published: November 2024

Abstract
Background and aim
Pathogenic Escherichia coli is a known harmful microorganism that takes advantage of favorable conditions to cause various infections in healthcare settings, such as bloodstream infections related to catheters, as well as infections in the urinary and respiratory tracts. E. coli utilizes biofilm development as a means to enhance its virulence and pathogenicity. This work aims to investigate the antibiofilm activity of α-amylase enzyme against uropathogenic E. coli (UPEC) and its effect on biofilm-regulatory genes.

Methodology
In this study, we evaluated the antibiofilm activity of α-amylase enzyme by spectrophotometric microtiter plate analysis and confocal laser scanning microscopy. Also, the antibacterial activity of the test enzyme was evaluated by measuring the MIC and MBC levels against UPEC. The quorum-quenching activity of α-amylase enzyme was assessed using a qRT-PCR to evaluate the impact on biofilm-regulatory genes.

Results
Based on our results, purified α-amylase showed MIC and MBC levels ranged between 128 and 512 µg /ml against UPEC isolates using broth microdilution assay. Crystal violet assay revealed MBIC of 128 µg/ml and MBEC of 256 µg/ml for the purified α-amylase. When the biofilm was analyzed by confocal laser scanning microscope, our results showed inhibition of biofilm thickness (56%) and live/dead cell percentages (43/55%). Furthermore, analysis of the effect on the expression of biofilm-encoding genes showed downregulation of both fimH and papC genes by 57 and 25%, respectively, upon treatment of UPEC with ½ of the MIC (64 µg/ml).

Conclusions
The results demonstrate that our purified α-amylase from B. cereus exhibits promising antibiofilm activities against UPEC at both phenotypic as well as genotypic levels. These findings suggest that this enzyme may serve as a natural effective tool for removing bacterial biofilms, potentially offering new therapeutic avenues for treating infections caused by UPEC.
Promoting healthy practices among schools and children in rural bangladesh: a randomised controlled trial of skill-based health education
Authors: Omura Makiko , Venkatesh Mohini , Khandaker Ikhtiar , Rahman Md. Ataur 
Published: November 2024

AbstractBackgroundPoor child health and hygiene practices are persistent issues in resource-constrained settings, particularly in low-income countries. This study assessed the impact of skill-based health education (SBHE) on school and child hygiene practices in rural Bangladesh.MethodsA cluster-randomised-controlled intervention with cross-cutting/factorial design was conducted in 180 randomly selected primary schools, stratified by school type, in Jhenaidah District, Bangladesh. Weekly SBHE sessions were delivered to half of the schools by locally recruited para-teachers for one year. A cross-cutting soap provision treatment was given monthly to half of the SBHE-treatment schools and half of the SBHE-control schools. Treatment assignment was masked to all baseline and endline surveyors. Data were collected at both the school and child levels, with child-level data aggregated at the school level. Outcome measures were grouped into five thematic families. The primary outcome families wereschool hygiene practice & maintenance,school-aggregated child handwashingandschool-aggregated child dentalcare. Utilising the difference-in-differences estimator with seemingly unrelated regression, we estimated the average treatment effect for each family of multiple outcomes. The intervention spillover effect to neighbouring schools along with the time-period effect were also evaluated. The project’s cost-effectiveness was additionally assessed.ResultsOur findings revealed that SBHE had a positive impact on primary outcomes related to healthy practices and behavioural changes, resulting in a 0.32SD improvement in school hygiene practices and maintenance (p < 0.001), a 0.47SD increase in child handwashing (p < 0.001), and a 0.43SD enhancement in child dentalcare (p < 0.01). Despite its imperfect implementation, the provision of soap itself showed no significant effect. Furthermore, significant spillover effects of healthy practices were observed in neighbouring non-treatment schools. The cost-effectiveness analysis indicated that our SBHE program was cost-effective.ConclusionsOur study provides compelling evidence of the positive impact of SBHE on school hygiene and child health practices in rural Bangladesh, with notable spillover effects. The cost-effectiveness analysis underscores the value of SBHE, affirming its potential as an effective intervention method in improving school health and hygiene practices in primary schools and beyond.
Clinical signs, morphological and phylogenetic characterization of Myxozoan spp. infecting Nile tilapia, Oreochromis niloticus and African catfish, Clarias gariepinus in Qalyubia Governorate, Egypt
Authors: Yassen Doaa A. , Abd El-Gawad Eman A. , Abd El-Razik Khaled A. , Mahrous Karima F. , Abbass Amany A. 
Published: November 2024

Abstract
Context
Myxosporean endoparasites (phylum cnidarian) are critical pathogens that affect both wild and cultured freshwater and marine water fishes globally causing huge economic losses and high mortalities.

Study objective
The present study investigated myxosporean infections in Nile tilapia and African catfish collected from the natural resources.

Methods
A total of four hundred Nile tilapia with an average weight (60 ± 5 g) and two hundred African catfish with an average weight (185 ± 30 g) were collected seasonally from Qalyubia Governorate, Egypt for parasitological and molecular diagnosis of isolated myxozoan species.

Results
Microscopic examination revealed Myxobolus heterosporous, Myxobolus brachysporus, Myxobolus tilapiae, and Myxobolus amieti in Nile tilapia and Henneguya suprabranchiae, and Myxobolus brachysporus in African catfish. Sequencing of 18S rDNA gene for isolated Myxozoan spp. from Nile tilapia revealed Myxobolus tilapiae deposited in GenBank under accession numbers (OR766325 and OR766326). In African catfish, the isolated Myxobolus brachysporus sequence was deposited under accession numbers (OR766327 and OR766328). Henneguya suprabranchiae was also identified in African catfish (accession. No. OR763724 and OR763433).

Conclusion
Overall, these results indicate a high prevalence of myxozoan infection in naturally inhabiting Nile tilapia and African catfish. Curiously, Henneguya suprabranchiae was detected in the digestive tract and kidneys of African catfish, which is considered a rare form.

Implication
This study highlighted the importance of parasitic surveys in natural resources that impact fish production.
Role of stretch-activated channels in light-generated action potentials mediated by an intramembrane molecular photoswitch
Authors: Florindi Chiara , Vurro Vito , Moretti Paola , Bertarelli Chiara , Zaza Antonio , Lanzani Guglielmo , Lodola Francesco 
Published: November 2024

Abstract
Background
The use of light to control the activity of living cells is a promising approach in cardiac research due to its unparalleled spatio-temporal selectivity and minimal invasiveness. Ziapin2, a newly synthesized azobenzene compound, has recently been reported as an efficient tool for light-driven modulation of excitation-contraction coupling (ECC) in human-induced pluripotent stem cells–derived cardiomyocytes. However, the exact biophysical mechanism of this process remains incompletely understood.

Methods
To address this, we performed a detailed electrophysiological characterization in a more mature cardiac model, specifically adult mouse ventricular myocytes (AMVMs).

Results
Our in vitro results demonstrate that Ziapin2 can photomodulate cardiac ECC in mature AMVMs without affecting the main transporters and receptors located within the sarcolemma. We established a connection between Ziapin2-induced membrane thickness modulation and light-generated action potentials by showcasing the pivotal role of stretch-activated channels (SACs). Notably, our experimental findings, through pharmacological blockade, suggest that non-selective SACs might serve as the biological culprit responsible for the effect.

Conclusions
Taken together, these findings elucidate the intricacies of Ziapin2-mediated photostimulation mechanism and open new perspectives for its application in cardiac research.
Loss of mitochondrial enzyme GPT2 leads to reprogramming of synaptic glutamate metabolism
Authors: Baytas Ozan , Davidson Shawn M. , Kauer Julie A. , Morrow Eric M. 
Published: November 2024

AbstractRecessive loss-of-function mutations in the mitochondrial enzyme Glutamate Pyruvate Transaminase 2 (GPT2) cause intellectual disability in children. Given this cognitive disorder, and because glutamate metabolism is tightly regulated to sustain excitatory neurotransmission, here we investigate the role of GPT2 in synaptic function. GPT2 catalyzes a reversible reaction interconverting glutamate and pyruvate with alanine and alpha-ketoglutarate, a TCA cycle intermediate; thereby, GPT2 may play an important role in linking mitochondrial tricarboxylic acid (TCA) cycle with synaptic transmission. In mouse brain, we find that GPT2 is enriched in mitochondria of synaptosomes (isolated synaptic terminals). Loss of Gpt2 in mouse appears to lead to reprogramming of glutamate and glutamine metabolism, and to decreased glutamatergic synaptic transmission. Whole-cell patch-clamp recordings in pyramidal neurons of CA1 hippocampal slices from Gpt2-null mice reveal decreased excitatory post-synaptic currents (mEPSCs) without changes in mEPSC frequency, or importantly, changes in inhibitory post-synaptic currents (mIPSCs). Additional evidence of defective glutamate release included reduced levels of glutamate released from Gpt2-null synaptosomes measured biochemically. Glutamate release from synaptosomes was rescued to wild-type levels by alpha-ketoglutarate supplementation. Additionally, we observed evidence of altered metabolism in isolated Gpt2-null synaptosomes: decreased TCA cycle intermediates, and increased glutamate dehydrogenase activity. Notably, alterations in the TCA cycle and the glutamine pool were alleviated by alpha-ketoglutarate supplementation. In conclusion, our data support a model whereby GPT2 mitochondrial activity may contribute to glutamate availability in pre-synaptic terminals, thereby highlighting potential interactions between pre-synaptic mitochondrial metabolism and synaptic transmission.