bluuebunny/crossref_metadata_2025_split huggingface dataset:
Altered mitochondrial mass and low mitochondrial membrane potential of immune cells in patients with HBV infection and correlation with liver inflammation
Authors: Ma Liling , Han Qingzhen , Cheng Longji , Song Huafeng , Qiang Rui , Xu Ping , Gao Fei , Zhu Li , Xu Junchi 
Published: November 2024

IntroductionMitochondrial membrane potential (MMP) and mitochondrial mass (MM) affect mitochondrial function and lymphocyte activation, but few studies on HBV infection exist. This study aimed to investigate the regulatory mechanism of mitochondrial dysfunction during HBV infection and its clinical significance by analyzing the alterations of MM and MMPlow in peripheral blood immune cells.MethodsThe study enrolled 90 participants, including healthy volunteers(HC) and patients with HBV infection, HBV patients were divided into chronic hepatitis B patients (CHB) and liver cirrhosis (LC) according to the study, and CHB was also divided into an inflammation group and a non-inflammation group. Flow cytometry was used to analyze the changes of MM and MMPlow in peripheral blood immune cells. These analyses were correlated with the presence of CHB and LC and indexes related to liver inflammation.ResultsThe study revealed significant variations in the percentage of MMPlow and MM of CD8+T cells associated with the progression of the disease. The MMPlow percentage of CD8+T cells in the LC group exhibited a notable decrease compared to the HC group and CHB groups. Moreover, MMPlow of CD8+T cells demonstrated potential in distinguishing CHB and LC (AUC=0.7341, P=0.0032). Furthermore, in exploring the link between mitochondrial function of immune cells and liver inflammation, the study found a negative correlation between the MMPlow ratio of CD4+T and CD8+T cells and AST (p=0.0039 and P=0.0070, r=-0.4405 and r=-0.4146), while the MM of CD8+T cells displayed a positive correlation with AST (p=0.0013, r=0.4865). In CHB patients with normal ALT but liver inflammation detected on B-scan ultrasonography, a significant decrease was observed in the MMPlow percentage of CD8+T (66.13 ± 14.27), CD56+NK(57.77 ± 17.40) and CD4-CD8-T (61.98 ± 15.98) cells. Furthermore, it was also found that the percentage of MMPlow in CD4-CD8-T cells could serve as an indicator for early liver inflammation and injury (AUC=0.8408, P=0.0052).DiscussionIn this study, we conducted a systematic analysis of the percentage of lymphocyte MMPlow and MM in various stages of HBV infection. Our findings revealed a correlation between MMPlow and MM and early liver inflammation, as well as the progression of the infection. This study marked the first demonstration of the clinical diagnostic value of MMPlow and MM in HBV infection. Furthermore, this was the first study to discuss the mitochondria of lymphocytes and liver inflammation in HBV infection. It enhanced the understanding of the role of T cells in liver inflammation, and elucidated potential markers for the early detection of liver injury and clinical cirrhosis.
Grain size of Quaternary sediments in the continental shelf-margin: implications for paleo-environment in the Northwestern South China Sea
Authors: Ge Jiawang , Li Qingping , Zhao Xiaoming , Pang Weixin , Fan Qi , Cheng Xiang , Zhang Xin 
Published: November 2024

IntroductionThe quantitative distribution of grain size of sediments could imply the hydrodynamic conditions as well as terrestrial material composition; and thus, it is indicative of sea-level fluctuations, regional sources and climate changes. The environmentally sensitive components extracted from grain size data serve as excellent indicators of the sedimentary environment and monsoon intensity.Material and methodsThe drilling data from the shelf margin of the northwestern Qiongdongnan Basin provide an excellent opportunity for studying hydrodynamics and climate change in the Quaternary South China Sea (SCS). The 49 obtained samples of Quaternary sediments are primarily composed of clay and silt, with a low sand content. The environmentally sensitive components are extracted from the sediment samples, based on multiple attempts including grain size-standard deviation, the end-member modelling analysis and the principal component factor analysis methods.ResultsThe increased grain size as supplemented by ratios of rolling movement on the sediment probability accumulation curves indicate enhanced hydrodynamic conditions in the Quaternary northwestern SCS. The alternative indicators of the Quaternary East Asian monsoon are obtained after a comprehensive comparative analysis. The changes in the content of the grain size components of 5.21-6.72 μm and 27.4-35.3 μm are used as the proxy indicators for the Quaternary East Asian summer and winter monsoon of the NW-SCS, respectively. It is likely indicated that the East Asian winter monsoon remarkably strengthened since 1.3 Ma but reached its maximum intensity around 0.8 Ma. During this period, the magnitude of both climatic temperature and sea-level fluctuations are significant, thus, the coarse-grained component increased at falling or low sea-level stages.DiscussionThe grain size characteristics of the Quaternary shelf margin sediments are indicative of hydrodynamic conditions, source-sink systems and environmental monsoon climate changes in the northwestern SCS.
Efficacy and safety of eravacycline versus tigecycline for complicated intra-abdominal infections in the ICU: a multicenter, single-blind, parallel randomized controlled trial study protocol
Authors: Liu Jin Jie , Guo Dong Dong , Wang Meng Xing , Li Yan Zhao , Li Hang , Liu Si Bo , Yang Rong Li , Zhang Dian Hong 
Published: November 2024

BackgroundComplicated intra-abdominal infections (cIAIs), often caused by multidrug-resistant bacteria such as carbapenem-resistant A. baumannii (CRAB) and carbapenem-resistant Enterobacteriaceae (CRE) are a critical challenge in ICUs. Owing to their high mortality and treatment failure rates, there is an urgent need for effective therapies. This trial will compare eravacycline to tigecycline for treating cIAIs in patients in the ICU, aiming to provide a superior treatment option.MethodsThis is a multicenter, single-blind, parallel randomized controlled trial. Adult patients in the ICU with complex abdominal infections who meet the eligibility criteria will be included. The main outcome is the all-cause 30-day mortality of patients in clinically evaluable and microbiologically evaluable populations. Secondary outcomes include the proportion of total responsive patients in the clinically evaluable population at the end of treatment and test of cure visits; the proportion of total responsive patients in the microbiologically evaluable population at the end of treatment and test of cure visits; and ICU hospitalization time and costs. Safety assessments include the incidence of various adverse events and changes in clinical laboratory test results. The subjects will be randomly assigned to receive treatment with either eravacycline or tigecycline at a 1:1 ratio. The all-cause mortality rates of patients treated with eravacycline and TGC were 17.7 and 18.7%, respectively, with an estimated actual mortality rate of 0.95. A total sample size of 262 subjects is required to reach 80% power with an α of 0.05. Considering a 10% loss rate, 292 patients will be enrolled and randomly assigned to the three groups in equal proportions.Ethics and communicationThis trial was approved by the Ethics Committee of Ansteel Group General Hospital. The communication plan includes presentations at scientific conferences, scientific publications, and presentations to the public through nonprofessional media.Clinical trial registrationhttps://clinicaltrials.gov/, ChiCTR2300078646.
Visualization of argininosuccinate synthetase by in silico analysis: novel insights into citrullinemia type I disorders
Authors: Gu Xia , Mo Wenhui , Zhuang Guiying , Shi Congcong , Wei Tao , Zhang Jinze , Tu Chiaowen , Cai Yao , Liao Biwen , Hao Hu 
Published: November 2024

BackgroundCitrullinemia type I disorders (CTLN1) is a genetic metabolic disease caused by argininosuccinate synthetase (ASS1) gene mutation. To date, the human genome mutation database has documented over 100 variants of the ASS1 gene. This study reported a novel deletion-insertion variant of ASS1 gene and employed various prediction tools to determine its pathogenicity.MethodsWe reported a case of early-onset CTLN1. Whole exome sequencing was conducted to identify genetic mutations. We employed various structure prediction tools to generate accurate 3D models and utilized computational biology tools to elucidate the disparities between the wild-type and mutant proteins.ResultsThe patient was characterized by severe clinical manifestations, including poor responsiveness, lethargy, convulsions, and cardiac arrest. Notably, the patient exhibited significantly elevated blood ammonia levels (655 μmol/L; normal reference: 10–30 μmol/L) and increased citrulline concentrations (936 μmol/L; normal reference: 5–25 μmol/L). Whole exome sequencing revealed a in-frame deletion-insertion mutation c.1128_1134delinsG in the ASS1 gene of unknown significance, which has not been previously reported. Our finding indicated that the C- terminal helix domain of the mutant protein structure, which was an important structure for ASS1 protein to form protein tetramers, was indeed more unstable than that of the wild-type protein structure.ConclusionThrough conducting an in silico analysis on this unique in-frame deletion-insertion variant of ASS1, our aim was to enhance understanding regarding its structure-function relationship as well as unraveling the molecular mechanism underlying CTLN1.
What specific exercise training is most effective exercise training method for patients on maintenance hemodialysis with sarcopenia: a network meta-analysis
Authors: Li Ying , Li Jingjing , Chen Xiaoan , Shi Yuegong , Shen Jie , Huang Ting 
Published: November 2024

ObjectiveThe present study aimed to investigate the influence of different exercise methods on sarcopenia patients receiving maintenance hemodialysis (MHD) by conducting a network meta-analysis.MethodsThe PubMed, Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure (CNKI), and Wanfang databases were searched online for relevant articles published until May 2024. Based on the inclusion and exclusion criteria, we selected 10 articles that compared the effects of 7 exercise interventions on sarcopenia patients receiving MHD.ResultsThe results of network meta-analysis showed that resistance training (RT) [standardized mean difference (SMD) = 4.54; 95% confidence interval (CI): 3.27–5.80] significantly improved the handgrip strength (HGS) of sarcopenia patients receiving MHD as compared to Baduanjin exercise (BAE) (SMD = 4.19; 95% CI: 2.31–6.07), bicycle exercise (BIE) (SMD = 4.06; 95% CI: 0.02–8.10), and combined movement (CE) (SMD = 3.50; 95% CI: 3.13–3.87). Compared to normal care (NC), BAE (SMD = 0.15; 95% CI: 0.07–0.23), RT (SMD = 0.34; 95% CI: 0.06–0.62), and CE (SMD = 0.37; 95% CI: 0.16–0.58) significantly improved skeletal muscle mass index (SMI) in sarcopenia patients receiving MHD.ConclusionThis study showed that RT has a positive effect on improving HGS in sarcopenia patients receiving MHD. CE also showed good results in enhancing SMI in MHD patients with sarcopenia. More randomized controlled trials are required to better understand the effectiveness of these exercise interventions and the potential underlying mechanisms.
Current status of short video as a source of information on lung cancer: a cross-sectional content analysis study
Authors: Zhao Xinyu , Yao Xinyi , Sui Binbin , Zhou Yutao 
Published: November 2024

BackgroundThe morbidity and mortality rates of lung cancer continue to rise, leading to a significant disease burden. Health education on lung cancer serves as an effective approach for prevention and treatment. With the increasing popularity of the Internet, an escalating number of patients are turning to video platforms for health information. Short videos facilitate better absorption and retention of information, thus becoming the primary channel for health education communication. However, the quality of information provided in videos on these platforms remains uncertain. Therefore, this study aims to assess the information quality pertaining to lung cancer in short videos available on a Chinese video platform.MethodsLung cancer-related videos on two short video platforms (TikTok and Kwai) were screened, and only Chinese (Mandarin) videos were included. The Global Quality Score (GQS) and modified DISCERN (mDISCERN) tools were then used to evaluate the quality and reliability of the information. A comparative analysis was conducted on videos from various sources. Additionally, correlation analysis was employed to investigate the factors influencing video quality.ResultsAfter screening, a total of 186 videos were included. The median GQS score and mDISCERN score were 3 (IQR: 3-4) and 2 (IQR: 2-4), respectively. A total of 44.1% of the lung cancer videos provided a comprehensive explanation of the symptoms, while only 3.2% fully explanation the complications associated with lung cancer. Health professionals, particularly specialists, demonstrated higher quality video information compared to individual users (P&lt;0.001). The correlation coefficient between GQS score and mDISCERN score was 0.340, showing a significant positive correlation (P&lt;0.001). In addition, GQS score was positively correlated with video duration (r=0.177, P=0.015)ConclusionThe information quality of the 186 videos screened by the two platforms in this study was generally unsatisfactory. However, videos provided by experts were deemed relatively reliable, with video duration being closely associated with information quality. Therefore, it is crucial to meticulously screen high-quality and dependable videos on the platform in order to effectively guide lung cancer prevention and treatment.
SLNP-based CDK4- targeted nanotherapy against glioblastoma
Authors: Ghani Uzma , Ghori Fareeha Khalid , Qamar Muhammad Usman , Khan Hina , Azad Basit , Habib Sabahat , Justin Saira , Khan Ishaq N. , Shah Tawaf Ali , Shazly Gamal A. , Bourhia Mohammed , Perveen Fouzia , Javed Aneela 
Published: November 2024

IntroductionGlioblastoma is a grade IV solid brain tumor and has a 15-month survival rate even after treatment. Glioblastoma development is heavily influenced by retinoblastoma protein (pRB) pathway changes. The blood–brain barrier, drug resistance, and severe toxicity of Temozolamide are key obstacles in treating glioblastoma. Innovative treatments targeting the pRB pathway with efficient delivery vehicles are required to treat glioblastoma.MethodsFor this purpose, a library of 691 plant extracts previously tested in vitro for anti-cancerous, anti inflammatory, and anti-proliferative characteristics was created after thorough literature investigations. Compounds were docked against pRB pathway protein ligands using molecular operating environment and chimera. Their nuclear structure and drug-like properties were predicted through Lipinski rule and density functional theory analysis. Physio-chemical characterizations of naked and drug-encapsulated SLNPs assessed size, stability, entrapment efficiency, and drug release rate. Anti-cancer potential of drug and drug- loaded SLNPs was evaluated using U87, U251, and HEK cell lines. Formulations were tested for cancer cell metastatic potential using cell migration assays.ResultsSilymarin (Sil) was identified as the most potent compound against CDK4, which was then encapsulated in stearic acid solid lipid nanoparticles (SLNP-Sil). Sil showed decreased cell viability 72 h after treatment against both U87 and U251 cell lines but had negligible cytotoxic effect on HEK-293. IC50 value of Sil was 155.14 µM for U87 and 195.93 µM for U251. Sil and SLNP-Sil effectively inhibited U87 and U251 cell migration 24 h after treatment.DiscussionOur results indicated that Sil and SLNP-Sil are promising therapeutic approaches against glioblastoma and merit in vivo experimental verification using orthotropic xenograft mouse models against glioblastoma.
Development and validation of a nomogram prediction model for clinically significant prostate cancer combined with PI-RADS V2.1, MRI quantitative parameters and clinical indicators: a two-center study
Authors: Chen Yunhui , Yan Long , Xianmei Jiang , Heyi Gu , Wei Xie , Chao Peng , Yanwen Dong , Shicun Dong , Chao Gao , Cui Yu , Peng Gu , Xiaodong Liu , xiaoyu Tuo , Bingbing Ling , Wenqing Ji , Kexian Gao , Qingqing Li , Linglin Zheng , Yun Zhu , Lei Zhao , Jihong Hu , Wei Zhao , Yaying Yang , Juan Hu 
Published: November 2024

ObjectiveTo develop and validate a multi-index nomogram prediction model for clinically significant prostate cancer(CSPCa) by combining the PI-RADS V2.1, quantitative magnetic resonance imaging (MRI) parameters and clinical indicators.MethodsA total of 1740 patients (75% in the derivation cohort and 25% in the internal validation cohort) and 342 patients (the external validation cohort) were retrospectively included in the MRI follow-up database of the First Affiliated Hospital of Kunming Medical University between January 2015 and April 2021,and Gejiu People’s Hospital between January 2020 and December 2022.Important predictors of CSPCa in MRI-related quantitative parameters, PSA-derived indicators, and clinical indicators, such as age, were screened. The Net Reclassification Improvement Index(NRI),Integrated Discrimination Improvement Index(IDI), and clinical decision curve analysis (DCA) were calculated to compare the performances of the different models. Receiver operating characteristic(ROC) curves and clinical calibration curves were used to analyze and compare diagnostic effects.ResultsThe AUC value, best cut-off value, specificity, sensitivity and accuracy of model 1(PI-RADS + PSAD) derivation cohort were 0.935, 0.304, 0.861, 0.895 and 0.872, respectively. The AUC values of the internal and external validation cohorts for model 1 were 0.956 and 0.955, respectively. The AUC value, best cut-off value, specificity, sensitivity and accuracy of model 2(PI-RADS +PSAD + ADCmean) derivation cohort were 0.939, 0.401, 0.895, 0.853 and 0.882, respectively. The AUC values of the internal and external validation cohorts for model 2 were 0.940 and 0.960,respectively. After adding the ADCmean to the model, the NRI(categorical), NRI(continuous) and IDI values were 0.0154, 0.3498 and 0.0222, respectively. There was no significant difference between the predicted probability and actual probability (p&gt; 0.05).ConclusionModels 1 and 2 had reliable, efficient and visual predictive value for CSPCa. The ADCmean is an important predictive indicator.
The efficacy of neoadjuvant immunotherapy in gastric cancer, adenocarcinoma of the esophagogastric junction, and esophageal cancer: a meta-analysis
Authors: Qian Mengyi , Fang Yingying , Xiang Zhiyi , Zhang Yueming , Zhan Hujie , Chen Xiaotong , Chen Yihang , Xu Tinghui 
Published: November 2024

BackgroundNeoadjuvant immunotherapy holds promise in managing resectable locally advanced gastric cancer (GC), adenocarcinoma of the esophagogastric junction (AEG), and esophageal cancer (EC). However, consensus is lacking regarding the efficacy of programmed death-1 (PD-1) and programmed death ligand 1 (PD-L1) inhibitors in neoadjuvant immunochemotherapy (NICT). This study aims to assess the added benefit of PD-1/PD-L1 inhibitors in neoadjuvant chemotherapy (NCT) for these malignancies.MethodsUp to October 2024, randomized controlled trials, case-control studies, and cohort studies that evaluated the addition of PD-1/PD-L1 inhibitors to NCT were systematically retrieved from electronic databases. The primary endpoints included pathologic complete response (pCR), major pathological response (MPR), overall survival (OS), and progression-free survival (PFS).ResultsThirteen studies published between 2021 and 2024 were analyzed. Statistical analyses revealed significantly higher pCR rates (OR: 2.73, P &lt; 0.001) and MPR rates (OR: 2.99, P &lt; 0.001) in the NICT group compared to NCT group. The PFS was also higher in the NICT group, although the difference did not reach statistical significance (HR: 0.50, P = 0.072).ConclusionThis meta-analysis demonstrates that NICT enhances pathological response rates in patients with resectable locally advanced GC, AEG, and EC. However, no significant long-term prognostic benefits were associated with NICT.Systematic review registrationhttps://www.crd.york.ac.uk/prospero, identifier CRD42024545725.
Peak systolic blood pressure during preparticipation exercise testing in 12,083 athletes: age, sex, and workload-indexed values and predictors
Authors: Pesova Petra , Jiravska Godula Bogna , Jiravsky Otakar , Jelinek Libor , Sovova Marketa , Moravcova Katarina , Ozana Jaromir , Ranic Ivan , Neuwirth Radek , Miklik Roman , Pekar Matej , Sknouril Libor , Tuka Vladimir , Sovova Eliska 
Published: November 2024

AimAssessment of blood pressure during exercise is routine in athletes, but normal values remain equivocal. This study examines the response of systolic blood pressure (SBP) to exercise in a large cohort of athletes and establishes normative values by sex and age.MethodsCompetitive athletes free of cardiovascular disease underwent pre-participation exercise testing on a bicycle ergometer. Resting (SBPrest) and peak blood pressure (SBPpeak), heart rate (HRrest and HRpeak), and power output (WR) were recorded. Workload indexed values were calculated.ResultsThe cohort included 12,083 athletes (median age 15 years, 26.9% female). Median peak exercise SBP was similar between sexes, but WR-indexed measures including SBP/WR ratio and SBP/(WR/kg) slope were higher in females (0.9 vs. 0.7, p &lt; 0.001; 10.94 vs. 9.52, p &lt; 0.001). Univariate analyses revealed significant associations between SBPpeak and several predictors, including sex, age, weight, height, SBPrest, DBPrest, HRrest, HRpeak, and WR (all p &lt; .001). Multivariate analysis showed that SBPrest (beta = 0.353, 95% CI [0.541, 0.609], p &lt; 0.001), height (beta = 0.303, 95% CI [0.360, 0.447], p &lt; 0.001), WR (beta = 0.171, 95% CI [0.029, 0.045], p &lt; 0.001), and age (beta = 0.093, 95% CI [0.162, 0.241], p &lt; 0.001) were the strongest predictors of SBPpeak.ConclusionThis study provides reference values for the interpretation of SBP responses to exercise in athletes. Multivariate analyses highlight the complex interplay of factors influencing peak SBP, including SBPrest, height, WR, age, DBPrest, sex, endurance sport category, and weight. In future studies, these findings may inform the development of personalised training strategies and risk stratification models in athletic populations.