bluuebunny/crossref_metadata_2025_split huggingface dataset:
WOR-TE: Ein Ja / Nein-Wortschatztest für Kinder verschiedener Altersgruppen
Authors: Trautwein Jutta , Schroeder Sascha 
Published: September 2018

Zusammenfassung. In dem vorliegenden Artikel wird der Wortschatztest WOR-TE für deutsche Grundschulkinder vorgestellt. Der Test basiert auf der Ja / Nein-Methode, bei der die Teilnehmerinnen und Teilnehmer aus einer Liste von Wörtern und Pseudowörtern diejenigen ankreuzen sollen, die sie kennen. Er wurde für verschiedene Altersgruppen (1./2. Klasse, 3./4. Klasse, 5./6. Klasse) konzipiert und Item-Response-Theory-basiert mit dem Rasch-Modell validiert. Anhand des Validitätskonzepts nach Messick (1995) wurden verschiedene Aspekte von Konstruktvalidität untersucht: Inhaltliche Aspekte, Relevanz, Repräsentativität, Technische Qualität, substantielle Aspekte, Generalisierbarkeit und externe Aspekte. Die Ergebnisse zeigen, dass der Test ein valides Instrument zur Erfassung des orthographischen Wortschatzes im Grundschulalter darstellt und durch kleine Veränderungen, insbesondere in Bezug auf die Item-Auswahl, profitieren würde. Möglichkeiten des Einsatzes sowie Einschränkungen des Verfahrens werden diskutiert, ebenso wie die Verwendung des Validitätskonzeptes mit IRT für Ja / Nein-Test im Allgemeinen.
Bottom‐Slope‐Induced Net Sediment Transport Rate Under Oscillatory Flows in the Rippled‐Bed Regime
Authors: Wang Dongxu , Yuan Jing 
Published: September 2018

AbstractFull‐scale experiments in the rippled‐bed regime were conducted in an oscillatory water tunnel to study the net sediment transport rate due to oscillatory flows on sloping bottoms (up to 2.6°). The tests cover a variety of sinusoidal flows, under which uniform two‐dimensional ripples are generated from a coarse sand movable bed. A laser‐based bottom profiler was developed to measure ripple geometry and net sediment transport rate. Under the same flow condition, a small bottom slope can slightly change the shape of the equilibrium ripples; for example, ripples become upslope leaning, but the ripple dimensions remain almost unchanged. Ripples are also found to migrate in the downslope direction with uniform speed and permanent shape, which is due to the spatial variation of net bedload transport rate. For a given flow condition, the slope‐induced net sediment transport rates are in the downslope direction and increase linearly with the bottom slope. A conceptual model, which is an extension of a sheet flow model (Yuan, Li, & Madsen, 2017, https://doi.org/10.1002/2016JC011996), was also developed. This calibration‐free model can reasonably predict the net transport rate, despite that a moderate underestimate (a factor slightly less than 2) is observed. Both our experiments and model suggest that the presence of ripples significantly enhances sediment mixing and leads to a large net suspended‐load transport rate, which will not occur under sheet flow conditions. Therefore, net transport rate in the ripple‐bed regime can be comparable to that in the sheet flow regime, although the flow condition is much weaker.
Lifetime cognition and late midlife blood metabolites: findings from a British birth cohort
Authors: Proitsi Petroula , Kuh Diana , Wong Andrew , Maddock Jane , Bendayan Rebecca , Wulaningsih Wahyu , Hardy Rebecca , Richards Marcus 
Published: September 2018

AbstractMaintenance of healthy cognitive ageing is vital for independence and wellbeing in the older general population. We investigated the association between blood metabolites and cognitive function and decline. Participants from the MRC National Survey of Health and Development (NSHD, the British 1946 birth cohort) were studied; 233 nuclear magnetic resonance circulating metabolite measures were quantified in 909 men and women at ages 60–64. Short-term and delayed verbal memory and processing speed were concurrently assessed and these tests were repeated at age 69. Linear regression analyses tested associations between metabolites and cognitive function at ages 60–64, and changes in these measures by age 69, adjusting for childhood cognition, education, socio-economic status and lifestyle factors. In cross-sectional analyses, metabolite levels, particularly fatty acid composition and different lipid sub-classes, were associated with short-term verbal memory (4 measures in females and 11 measures in the whole sample), delayed verbal memory (2 measures in females) and processing speed (8 measures in males and 2 measures in the whole sample) (p < 0.002). One metabolite was associated with change in cognition in females. Most of the observed associations were attenuated after adjustment for childhood cognition and education. A life course perspective can improve the understanding of how peripheral metabolic processes underlie cognitive ageing.
HMGB1 promotes ERK-mediated mitochondrial Drp1 phosphorylation for chemoresistance through RAGE in colorectal cancer
Authors: Huang Chih-Yang , Chiang Shu-Fen , Chen William Tzu-Liang , Ke Tao-Wei , Chen Tsung-Wei , You Ying-Shu , Lin Chen-Yu , Chao K. S. Clifford , Huang Chih-Yang 
Published: September 2018

AbstractDysfunctional mitochondria have been shown to enhance cancer cell proliferation, reduce apoptosis, and increase chemoresistance. Chemoresistance develops in nearly all patients with colorectal cancer, leading to a decrease in the therapeutic efficacies of anticancer agents. However, the effect of dynamin-related protein 1 (Drp1)-mediated mitochondrial fission on chemoresistance in colorectal cancer is unclear. Here, we found that the release of high-mobility group box 1 protein (HMGB1) in conditioned medium from dying cells by chemotherapeutic drugs and resistant cells, which triggered Drp1 phosphorylation via its receptor for advanced glycation end product (RAGE). RAGE signals ERK1/2 activation to phosphorylate Drp1 at residue S616 triggerring autophagy for chemoresistance and regrowth in the surviving cancer cells. Abolishment of Drp1 phosphorylation by HMGB1 inhibitor and RAGE blocker significantly enhance sensitivity to the chemotherapeutic treatment by suppressing autophagy. Furthermore, patients with high phospho-Drp1Ser616 are associated with high risk on developing tumor relapse, poor 5-year disease-free survival (DFS) and 5-year overall survival (OS) after neoadjuvant chemoradiotherapy (neoCRT) treatment in locally advanced rectal cancer (LARC). Moreover, patients with RAGE-G82S polymorphism (rs2070600) are associated with high phospho-Drp1Ser616 within tumor microenvironment. These findings suggest that the release of HMGB1 from dying cancer cells enhances chemoresistance and regrowth via RAGE-mediated ERK/Drp1 phosphorylation.
Metabolic profile and differentiation potential of extraembryonic endoderm-like cells
Authors: Gatie Mohamed I. , Kelly Gregory M. 
Published: September 2018

AbstractGlucose metabolism has a crucial role for providing substrates required to generate ATP and regulate the epigenetic landscape. We reported that F9 embryonal carcinoma stem-like cells require cytosolic reactive oxygen species to differentiate into extraembryonic endoderm; however, mitochondrial sources were not examined. To extend these studies, we examined the metabolic profile of early and late-passage F9 cells, and show that their ability to differentiate is similar, even though each population has dramatically different metabolic profiles. Differentiated early-passage cells relied on glycolysis, while differentiated late-passage cells transitioned towards oxidative phosphorylation (OXPHOS). Unexpectedly, electron transport chain protein stoichiometry was disrupted in differentiated late-passage cells, whereas genes encoding mitofusion 1 and 2, which promote mitochondrial fusion and favor OXPHOS, were upregulated in differentiated early-passage cells. Despite this, early-passage cells cultured under conditions to promote glycolysis showed enhanced differentiation, whereas promoting OXPHOS in late-passage cells showed a similar trend. Further analysis revealed that the distinct metabolic profiles seen between the two populations is largely associated with changes in genomic integrity, linking metabolism to passage number. Together, these results indicate that passaging has no effect on the potential for F9 cells to differentiate into extraembryonic endoderm; however, it does impact their metabolic profile. Thus, it is imperative to determine the molecular and metabolic status of a stem cell population before considering its utility as a therapeutic tool for regenerative medicine.
Cell cycle-resolved chromatin proteomics reveals the extent of mitotic preservation of the genomic regulatory landscape
Authors: Ginno Paul Adrian , Burger Lukas , Seebacher Jan , Iesmantavicius Vytautas , Schübeler Dirk 
Published: September 2018

AbstractRegulation of transcription, replication, and cell division relies on differential protein binding to DNA and chromatin, yet it is unclear which regulatory components remain bound to compacted mitotic chromosomes. By utilizing the buoyant density of DNA–protein complexes after cross-linking, we here develop a mass spectrometry-based approach to quantify the chromatin-associated proteome at separate stages of the cell cycle. While epigenetic modifiers that promote transcription are lost from mitotic chromatin, repressive modifiers generally remain associated. Furthermore, while proteins involved in transcriptional elongation are evicted, most identified transcription factors are retained on mitotic chromatin to varying degrees, including core promoter binding proteins. This predicts conservation of the regulatory landscape on mitotic chromosomes, which we confirm by genome-wide measurements of chromatin accessibility. In summary, this work establishes an approach to study chromatin, provides a comprehensive catalog of chromatin changes during the cell cycle, and reveals the degree to which the genomic regulatory landscape is maintained through mitosis.
Advanced sulfide solid electrolyte by core-shell structural design
Authors: Wu Fan , Fitzhugh William , Ye Luhan , Ning Jiaxin , Li Xin 
Published: September 2018

AbstractSolid electrolyte is critical to next-generation solid-state lithium-ion batteries with high energy density and improved safety. Sulfide solid electrolytes show some unique properties, such as the high ionic conductivity and low mechanical stiffness. Here we show that the electrochemical stability window of sulfide electrolytes can be improved by controlling synthesis parameters and the consequent core-shell microstructural compositions. This results in a stability window of 0.7–3.1 V and quasi-stability window of up to 5 V for Li-Si-P-S sulfide electrolytes with high Si composition in the shell, a window much larger than the previously predicted one of 1.7–2.1 V. Theoretical and computational work explains this improved voltage window in terms of volume constriction, which resists the decomposition accompanying expansion of the solid electrolyte. It is shown that in the limiting case of a core-shell morphology that imposes a constant volume constraint on the electrolyte, the stability window can be further opened up. Advanced strategies to design the next-generation sulfide solid electrolytes are also discussed based on our understanding.
Functional equivalence of genome sequencing analysis pipelines enables harmonized variant calling across human genetics projects
Authors: Regier Allison A. , Farjoun Yossi , Larson David E. , Krasheninina Olga , Kang Hyun Min , Howrigan Daniel P. , Chen Bo-Juen , Kher Manisha , Banks Eric , Ames Darren C. , English Adam C. , Li Heng , Xing Jinchuan , Zhang Yeting , Matise Tara , Abecasis Goncalo R. , Salerno Will , Zody Michael C. , Neale Benjamin M. , Hall Ira M. 
Published: September 2018

AbstractHundreds of thousands of human whole genome sequencing (WGS) datasets will be generated over the next few years. These data are more valuable in aggregate: joint analysis of genomes from many sources increases sample size and statistical power. A central challenge for joint analysis is that different WGS data processing pipelines cause substantial differences in variant calling in combined datasets, necessitating computationally expensive reprocessing. This approach is no longer tenable given the scale of current studies and data volumes. Here, we define WGS data processing standards that allow different groups to produce functionally equivalent (FE) results, yet still innovate on data processing pipelines. We present initial FE pipelines developed at five genome centers and show that they yield similar variant calling results and produce significantly less variability than sequencing replicates. This work alleviates a key technical bottleneck for genome aggregation and helps lay the foundation for community-wide human genetics studies.
Observing of the super-Planckian near-field thermal radiation between graphene sheets
Authors: Yang Jiang , Du Wei , Su Yishu , Fu Yang , Gong Shaoxiang , He Sailing , Ma Yungui 
Published: September 2018

AbstractThermal radiation can be substantially enhanced in the near-field scenario due to the tunneling of evanescent waves. Monolayer graphene could play a vital role in this process owing to its strong infrared plasmonic response, however, which still lacks an experimental verification due to the technical challenges. Here, we manage to make a direct measurement about plasmon-mediated thermal radiation between two macroscopic graphene sheets using a custom-made setup. Super-Planckian radiation with efficiency 4.5 times larger than the blackbody limit is observed at a 430-nm vacuum gap on insulating silicon hosting substrates. The positive role of graphene plasmons is further confirmed on conductive silicon substrates which have strong infrared loss and thermal emittance. Based on these, a thermophotovoltaic cell made of the graphene–silicon heterostructure is lastly discussed. The current work validates the classic thermodynamical theory in treating graphene and also paves a way to pursue the application of near-field thermal management.
Preventing acute asthmatic symptoms by targeting a neuronal mechanism involving carotid body lysophosphatidic acid receptors
Authors: Jendzjowsky Nicholas G. , Roy Arijit , Barioni Nicole O. , Kelly Margaret M. , Green Francis H. Y. , Wyatt Christopher N. , Pye Richard L. , Tenorio-Lopes Luana , Wilson Richard J. A. 
Published: September 2018

AbstractAsthma accounts for 380,000 deaths a year. Carotid body denervation has been shown to have a profound effect on airway hyper-responsiveness in animal models but a mechanistic explanation is lacking. Here we demonstrate, using a rat model of asthma (OVA-sensitized), that carotid body activation during airborne allergic provocation is caused by systemic release of lysophosphatidic acid (LPA). Carotid body activation by LPA involves TRPV1 and LPA-specific receptors, and induces parasympathetic (vagal) activity. We demonstrate that this activation is sufficient to cause acute bronchoconstriction. Moreover, we show that prophylactic administration of TRPV1 (AMG9810) and LPA (BrP-LPA) receptor antagonists prevents bradykinin-induced asthmatic bronchoconstriction and, if administered following allergen exposure, reduces the associated respiratory distress. Our discovery provides mechanistic insight into the critical roles of carotid body LPA receptors in allergen-induced respiratory distress and suggests alternate treatment options for asthma.
Total syntheses of shizukaols A and E
Authors: Wu Jian-Li , Lu Yin-Suo , Tang Bencan , Peng Xiao-Shui 
Published: September 2018

AbstractShizukaols possess a common heptacyclic framework containing more than ten contiguous stereocenters and potential biological activities. Here we report that the total syntheses of shizukaols A (1) and E (2), two lindenane-type dimers from the Chloranthaceae family, are achieved via a modified biomimetic Diels–Alder reaction. The common crucial biomimetic diene 23 and ethylene species (6, 17) are obtained through either a highly Z-selective olefination of α-siloxy ketone with ynolate anions or an intramolecular Horner–Wadsworth–Emmons olefination from commercially available Wieland–Miescher ketone (7). This synthetic approach here opens up practical avenues for the total syntheses of the intriguing Chloranthaceae family members, as well as the understanding of their relevant biological action in nature.
Zebra finches identify individuals using vocal signatures unique to each call type
Authors: Elie Julie E. , Theunissen Frédéric E. 
Published: September 2018

AbstractIndividual recognition is critical in social animal communication, but it has not been demonstrated for a complete vocal repertoire. Deciphering the nature of individual signatures across call types is necessary to understand how animals solve the problem of combining, in the same signal, information about identity and behavioral state. We show that distinct signatures differentiate zebra finch individuals for each call type. The distinctiveness of these signatures varies: contact calls bear strong individual signatures while calls used during aggressive encounters are less individualized. We propose that the costly solution of using multiple signatures evolved because of the limitations of the passive filtering properties of the birds’ vocal organ for generating sufficiently individualized features. Thus, individual recognition requires the memorization of multiple signatures for the entire repertoire of conspecifics of interests. We show that zebra finches excel at these tasks.
A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation
Authors: Sheng Chao , Jungverdorben Johannes , Wiethoff Hendrik , Lin Qiong , Flitsch Lea J. , Eckert Daniela , Hebisch Matthias , Fischer Julia , Kesavan Jaideep , Weykopf Beatrice , Schneider Linda , Holtkamp Dominik , Beck Heinz , Till Andreas , Wüllner Ullrich , Ziller Michael J. , Wagner Wolfgang , Peitz Michael , Brüstle Oliver 
Published: September 2018

AbstractRecent reports suggest that induced neurons (iNs), but not induced pluripotent stem cell (iPSC)-derived neurons, largely preserve age-associated traits. Here, we report on the extent of preserved epigenetic and transcriptional aging signatures in directly converted induced neural stem cells (iNSCs). Employing restricted and integration-free expression of SOX2 and c-MYC, we generated a fully functional, bona fide NSC population from adult blood cells that remains highly responsive to regional patterning cues. Upon conversion, low passage iNSCs display a profound loss of age-related DNA methylation signatures, which further erode across extended passaging, thereby approximating the DNA methylation age of isogenic iPSC-derived neural precursors. This epigenetic rejuvenation is accompanied by a lack of age-associated transcriptional signatures and absence of cellular aging hallmarks. We find iNSCs to be competent for modeling pathological protein aggregation and for neurotransplantation, depicting blood-to-NSC conversion as a rapid alternative route for both disease modeling and neuroregeneration.
RIP2 filament formation is required for NOD2 dependent NF-κB signalling
Authors: Pellegrini Erika , Desfosses Ambroise , Wallmann Arndt , Schulze Wiebke Manuela , Rehbein Kristina , Mas Philippe , Signor Luca , Gaudon Stephanie , Zenkeviciute Grasilda , Hons Michael , Malet Helene , Gutsche Irina , Sachse Carsten , Schoehn Guy , Oschkinat Hartmut , Cusack Stephen 
Published: September 2018

AbstractActivation of the innate immune pattern recognition receptor NOD2 by the bacterial muramyl-dipeptide peptidoglycan fragment triggers recruitment of the downstream adaptor kinase RIP2, eventually leading to NF-κB activation and proinflammatory cytokine production. Here we show that full-length RIP2 can form long filaments mediated by its caspase recruitment domain (CARD), in common with other innate immune adaptor proteins. We further show that the NOD2 tandem CARDs bind to one end of the RIP2 CARD filament, suggesting a mechanism for polar filament nucleation by activated NOD2. We combine X-ray crystallography, solid-state NMR and high-resolution cryo-electron microscopy to determine the atomic structure of the helical RIP2 CARD filament, which reveals the intermolecular interactions that stabilize the assembly. Using structure-guided mutagenesis, we demonstrate the importance of RIP2 polymerization for the activation of NF-κB signalling by NOD2. Our results could be of use to develop new pharmacological strategies to treat inflammatory diseases characterised by aberrant NOD2 signalling.
Two-dimensional amorphous NiO as a plasmonic photocatalyst for solar H2 evolution
Authors: Lin Zhaoyong , Du Chun , Yan Bo , Wang Chengxin , Yang Guowei 
Published: September 2018

AbstractAmorphous materials are usually evaluated as photocatalytically inactive due to the amorphous nature-induced self-trapping of tail states, in spite of their achievements in electrochemistry. NiO crystals fail to act as an individual reactor for photocatalytic H2 evolution because of the intrinsic hole doping, regardless of their impressive cocatalytic ability for proton/electron transfer. Here we demonstrate that two-dimensional amorphous NiO nanostructure can act as an efficient and robust photocatalyst for solar H2 evolution without any cocatalysts. Further, the antenna effect of surface plasmon resonance can be introduced to construct an incorporate antenna-reactor structure by increasing the electron doping. The solar H2 evolution rate is improved by a factor of 19.4 through the surface plasmon resonance-mediated charge releasing. These findings thus open a door to applications of two-dimensional amorphous NiO as an advanced photocatalyst.
Motor recruitment to the TIM23 channel’s lateral gate restricts polypeptide release into the inner membrane
Authors: Schendzielorz Alexander Benjamin , Bragoszewski Piotr , Naumenko Nataliia , Gomkale Ridhima , Schulz Christian , Guiard Bernard , Chacinska Agnieszka , Rehling Peter 
Published: September 2018

AbstractThe presequence translocase of the mitochondrial inner membrane (TIM23 complex) facilitates anterograde precursor transport into the matrix and lateral release of precursors with stop-transfer signal into the membrane (sorting). Sorting requires precursor exit from the translocation channel into the lipid phase through the lateral gate of the TIM23 complex. How the two transport modes are regulated and balanced against each other is unknown. Here we show that the import motor J-protein Pam18, which is essential for matrix import, controls lateral protein release into the lipid bilayer. Constitutively translocase-associated Pam18 obstructs lateral precursor transport. Concomitantly, Mgr2, implicated in precursor quality control, is displaced from the translocase. We conclude that during motor-dependent matrix protein transport, the transmembrane segment of Pam18 closes the lateral gate to promote anterograde polypeptide movement. This finding explains why a motor-free form of the translocase facilitates the lateral movement of precursors with a stop-transfer signal.