bluuebunny/crossref_metadata_2025_split huggingface dataset:
Masked hypertension and submaximal exercise blood pressure among adolescents from the Avon Longitudinal Study of Parents and Children (ALSPAC)
Authors: Huang Zhengzheng , Sharman James E. , Fonseca Ricardo , Park Chloe , Chaturvedi Nish , Davey Smith George , Howe Laura D. , Lawlor Deborah A. , Hughes Alun D. , Schultz Martin G. 
Published: July 2019

PurposeMasked hypertension is associated with increased cardiovascular risk but is undetectable by clinic blood pressure (BP). Elevated systolic BP responses to submaximal exercise reveal the presence of masked hypertension in adults, but it is unknown whether this is the case during adolescence. We aimed to determine if exercise BP was raised in adolescents with masked hypertension, and its association with cardiovascular risk markers.MethodsA total of 657 adolescents (aged 17.7 ± 0.3 years; 41.9% male) from the Avon longitudinal study of parents and children (ALSPAC) completed a step‐exercise test with pre‐, post‐, and recovery‐exercise BP, clinic BP and 24‐hour ambulatory BP. Masked hypertension was defined as clinic BP <140/90 mm Hg and 24‐hour ambulatory BP ≥130/80 mm Hg. Assessment of left‐ventricular (LV) mass index and carotid‐femoral pulse wave velocity (aortic PWV) was also undertaken. Thresholds of clinic, pre‐, post‐, and recovery‐exercise systolic BP were explored from ROC analysis to identify masked hypertension.ResultsFifty participants (7.8%) were classified with masked hypertension. Clinic, pre‐, post‐, and recovery‐exercise systolic BP were associated with masked hypertension (AUC ≥ 0.69 for all, respectively), with the clinic systolic BP threshold of 115 mm Hg having high sensitivity and specificity and exercise BP thresholds of 126, 150, and 130 mm Hg, respectively, having high specificity and negative predictive value (individually or when combined) for ruling out the presence of masked hypertension. Additionally, this exercise systolic BP above the thresholds was associated with greater left‐ventricular mass index and aortic PWV.ConclusionsSubmaximal exercise systolic BP is associated with masked hypertension and adverse cardiovascular structure in adolescents. Exercise BP may be useful in addition to clinic BP for screening of high BP and cardiovascular risk in adolescents.
AH2 encodes a MYB domain protein that determines hull fate and affects grain yield and quality in rice
Authors: Ren Deyong , Cui Yuanjiang , Hu Haitao , Xu Qiankun , Rao Yuchun , Yu Xiaoqi , Zhang Yu , Wang Yuexing , Peng Youlin , Zeng Dali , Hu Jiang , Zhang Guangheng , Gao Zhenyu , Zhu Li , Chen Guang , shen Lan , Zhang Qiang , Guo Longbiao , Qian Qian 
Published: July 2019

SummaryThe palea and lemma (hull) are grass‐specific organs, and determine grain size and quality. In the study, AH2 encodes a MYB domain protein, and functions in the development of hull and grain. Mutation of AH2 produces smaller grains and alters grain quality including decreased amylose content and gel consistency, and increased protein content. Meantime, part of the hull lost the outer silicified cells, and induces a transformation of the outer rough epidermis to inner smooth epidermis cells, and the body of the palea was reduced in the ah2 mutant. We confirmed the function of AH2 by complementation, CRISPR‐Cas9, and cytological and molecular tests. Additionally, AH2, as a repressor, repress transcription of the downstream genes. Our results revealed that AH2 plays an important role in the determination of hull epidermis development, palea identity, and grain size.
Comparative analysis of metabolome of rice seeds at three developmental stages using a recombinant inbred line population
Authors: Li Kang , Wang Dehong , Gong Liang , Lyu Yuanyuan , Guo Hao , Chen Wei , Jin Cheng , Liu Xianqing , Fang Chuanying , Luo Jie 
Published: July 2019

SummaryPlants are considered an important food and nutrition source for humans. Despite advances in plant seed metabolomics, knowledge about the genetic and molecular bases of rice seed metabolomes at different developmental stages is still limited. Here, using Zhenshan 97 (ZS97) and Minghui 63 (MH63), we performed a widely targeted metabolic profiling in seeds during grain filling, mature seeds and germinating seeds. The diversity between MH63 and ZS97 was characterized in terms of the content of metabolites and the metabolic shifting across developmental stages. Taking advantage of the ultra‐high‐density genetic map of a population of 210 recombinant inbred lines (RILs) derived from a cross between ZS97 and MH63, we identified 4681 putative metabolic quantitative trait loci (mQTLs) in seeds across the three stages. Further analysis of the mQTLs for the codetected metabolites across the three stages revealed that the genetic regulation of metabolite accumulation was closely related to developmental stage. Using in silico analyses, we characterized 35 candidate genes responsible for 30 structurally identified or annotated compounds, among which LOC_Os07g04970 and LOC_Os06g03990 were identified to be responsible for feruloylserotonin and l‐asparagine content variation across populations, respectively. Metabolite−agronomic trait association and colocation between mQTLs and phenotypic quantitative trait loci (pQTLs) revealed the complexity of the metabolite−agronomic trait relationship and the corresponding genetic basis.
Red blood cell alloimmunization is associated with lower expression of FcγR1 on monocyte subsets in patients with sickle cell disease
Authors: Balbuena‐Merle Raisa , Curtis Susanna A. , Devine Lesley , Gibb David R. , Karafin Matthew S. , Luckey Chance John , Tormey Christopher A. , Siddon Alexa J. , Roberts John D. , Hendrickson Jeanne E. 
Published: July 2019

BACKGROUNDDespite the clinical significance of red blood cell (RBC) alloantibodies, there are currently no laboratory tests available to predict which patients may be at risk of antibody formation after transfusion exposure. Given their phagocytic and inflammatory functions, we hypothesized that differences in circulating monocytes may play a role in alloimmunization.STUDY DESIGN AND METHODSForty‐two adults with sickle cell disease (SCD) were recruited, with data extracted from the electronic medical record and peripheral blood analyzed by flow cytometry for total monocytes, monocyte subsets (CD14 high/CD16 low+ classical monocytes, CD14 high/CD16 high+ intermediate monocytes, and CD14 intermediate/CD16 high+ non‐classical/inflammatory monocytes), and FcγR1 (CD64) expression. Thirteen “non‐responder” patients (non‐alloimmunized patients with documented RBC transfusion at the study institution) were compared to 20 alloimmunized “responder” patients, who had a total of 44 RBC alloantibodies identified.RESULTSThere were no significant differences in the percentages of total monocytes, monocyte subsets, or measured cytokines between non‐responders and responders. However, non‐responders had higher CD64 expression on classical monocytes (MFI mean 3424 ± standard deviation 1141) compared to responders (MFI mean 2285 ± 1501), p = 0.029, and on intermediate monocytes (MFI mean 3720 ± 1191) compared to responders (MFI mean 2497 ± 1640), p = 0.033.CONCLUSIONSMonocytes and the inflammatory milieu increasingly are being appreciated to play a role in some complications of SCD. The differences in FcγR1 expression on monocyte subsets noted between responders and non‐responders, which cannot be directly explained by the serum cytokines evaluated, warrant further investigation.
Fluorine‐18‐fluoro‐2‐deoxy‐d‐glucose PET‐CT aids in detection of soft‐tissue injuries for dogs with thoracic or pelvic limb lameness
Authors: Frank Ilan , Mann Kelly , Duerr Felix 
Published: July 2019

AbstractFluorine‐18‐fluoro‐2‐deoxy‐d‐glucose positron emission tomography (FDG PET) provides physiologic images of tissues based on their glucose metabolism. The combination of FDG PET and CT (FDG PET‐CT) has been utilized in human musculoskeletal imaging to localize soft tissue lesions, however, this modality has not been thoroughly investigated for the diagnosis of canine lameness. This prospective, descriptive study evaluated FDG PET‐CT findings in 25 client‐owned dogs with inconclusive origin of thoracic or pelvic limb lameness (thoracic limb n = 15/25, 60%; pelvic limb n = 6/25, 24%; and combination of both limbs n = 4/25, 16%). We hypothesized that FDG PET‐CT would aid the detection of soft tissue lesions not visible with other imaging modalities. Combined FDG PET‐CT detected soft tissue lesions in 40% (n = 10/25) and osteoarthritis in 64% (n = 16/25) of the patients. FDG PET detected more soft tissue lesions than contrast‐enhanced CT (n = 15/15, 100% and n = 12/15, 80%, respectively), while CT identified more osteoarthritis lesions than FDG PET (n = 26/26, 100% and n = 18/26, 69%, respectively). The three imaging‐diagnoses based on the FDG PET component included the following: flexor carpi ulnaris muscle tear, psoas major myopathy, and tarsal desmopathy. No diagnosis for the lameness was obtained in three dogs. Findings supported FDG PET‐CT as a useful adjunct imaging modality for detection of certain soft tissue injuries of the musculoskeletal system. Combined FDG PET‐CT should be considered for cases where the cause of lameness is thought to be of soft tissue origin and cannot be diagnosed by conventional means.
Post-operative pain management among surgical trauma patients in an acute ward: a best practice implementation project
Authors: García-Monasterio Emilia Irene , Alvárez-Vázquez Juan Carlos , Morado-Quiñoá Purificación , Pena-Pena Amparo , Mazón Mar Folgueira , Bouso Ana Murado , López Angeles Landeira , Cendán-Celeiro Elena 
Published: July 2019

ABSTRACT

Introduction:
Effective pain management should be one of the main goals of healthcare professionals. The trauma unit of a hospital in an urban area of Spain carried out an implementation project, guided by the evidence-based criteria from JBI, to put in place the processes and initiatives needed to reduce post-operative pain.

Objectives:
The aim of this implementation project was to promote evidence-based practice in managing post-operative pain.

Methods:
A pre-post implementation audit was implemented using the JBI Practical Application of Clinical Evidence System (PACES) and Getting Research into Practice (GRiP) tool. Each audit included 30 post-operative patients from a randomized sample who were evaluated before the project started and six months after key strategies had been implemented. The criteria were audited according to evidence-based process criteria.

Results:
At the baseline audit, the compliance percentages for the evidence-based criteria ranged from 10% to 43%. Seven obstacles were identified in relation to post-operative pain management. Following the GRiP table, the team established a series of strategies and resources to implement the improvement actions. When the implementation period ended, all the criteria had improved.

Conclusions:
The quality improvement cycle allowed us to implement the clinical best practice recommendations, with subsequent outcome improvements for patients. Future audits should be performed to drive new cycles of improvement in evidence-based practice.
Toward the Integrated Design of Organic Rankine Cycle Power Plants: A Method for the Simultaneous Optimization of Working Fluid, Thermodynamic Cycle, and Turbine
Authors: Lampe Matthias , De Servi Carlo , Schilling Johannes , Bardow André , Colonna Piero 
Published: July 2019

Abstract
The conventional design of organic Rankine cycle (ORC) power systems starts with the selection of the working fluid and the subsequent optimization of the corresponding thermodynamic cycle. More recently, systematic methods have been proposed integrating the selection of the working fluid into the optimization of the thermodynamic cycle. However, in both cases, the turbine is designed subsequently. This procedure can lead to a suboptimal design, especially in the case of mini- and small-scale ORC systems, since the preselected combination of working fluid and operating conditions may lead to infeasible turbine designs. The resulting iterative design procedure may end in conservative solutions after multiple trial-and-error attempts due to the strong interdependence of the many design variables and constraints involved. In this work, we therefore present a new design and optimization method integrating working fluid selection, thermodynamic cycle design, and preliminary turbine design. To this purpose, our recent 1-stage continuous-molecular targeting (CoMT)-computer-aided molecular design (CAMD) method for the integrated design of the ORC process and working fluid is expanded by a turbine meanline design procedure. Thereby, the search space of the optimization is bounded to regions where the design of the turbine is feasible. The resulting method has been tested for the design of a small-scale high-temperature ORC unit adopting a radial-inflow turbo-expander. The results confirm the potential of the proposed method over the conventional iterative design practice for the design of small-scale ORC turbogenerators.
Aerobic Training Performed at Ventilatory Threshold Improves Psychological Outcomes in Adolescents With Obesity
Authors: Fidelix Yara , Lofrano-Prado Mara C. , Fortes Leonardo S. , Hill James O. , Caldwell Ann E. , Botero João P. , do Prado Wagner L. 
Published: July 2019

Background: Physical activity may be as effective as some drugs for improving psychological outcomes; however, vigorous exercise may be needed for improving these outcomes in adolescents with obesity. The aim of this study is to examine the effects of low- and high-intensity training on self-esteem and symptoms of depression and anxiety in adolescents with obesity. Methods: A total of 62 pubertal adolescents with obesity (age 15 [1.5] y, body mass index 34.87 [4.22] kg/m2) were randomized into high-intensity group (HIG, n = 31) or low-intensity group (LIG, n = 31) for 24 weeks. All participants also received nutritional, psychological, and clinical counseling. Body composition and measures of depressive symptoms, anxiety, and self-esteem were assessed at baseline and after 24 weeks. Results: Depressive symptoms decreased significantly in both HIG (d = 1.16) and LIG (d = 0.45) (P ≤ .01). Trait anxiety decreased after 24 weeks for HIG (d = 0.81, P = .002) and LIG (d = 0.31, P = .002). No changes were observed in state anxiety or self-esteem. Conclusions: Results from the present study demonstrate that 24 weeks of multidisciplinary intervention improves depression and anxiety symptoms in adolescents with obesity; however, the magnitude of changes is higher in HIG compared with LIG.
Women’s occupational performance and quality of life during breast cancer treatment
Authors: Nascimento Pamela Ferreira , Mello Maria Julia Gonçalves , Correia Nancy de Barros , Lucena Nahãmi Cruz , Albuquerque Raquel Costa , de Matos Raisa Mayara Alves , Bergmann Anke 
Published: July 2019

ObjectivesTo analyse the association between occupational performance and women’s health-related quality of life (HRQoL) during breast cancer treatment.MethodsAn analytical cross-sectional study was conducted using the Canadian Occupational Performance Measure, the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire and its specific breast cancer-specific module (BR-23). The association between occupational performance scores and the HRQoL domains was evaluated using multiple linear regression analysis.ResultsThe sample included 97 women. Overall, occupational performance was poor and the women’s dissatisfaction with their performance resulted in a decrease in HRQoL. Following adjustment, satisfaction was associated with HRQoL in the global health status, functional, role, emotional and social functioning domains of the functional scale. In BR-23, a statistically significant association was found between satisfaction and HRQoL in the functional scale and in the body image domain.ConclusionsWomen’s occupational performance was impaired during breast cancer treatment, with limitations and/or restrictions in activities related to self-care, productivity and leisure that were ultimately associated with poorer HRQoL. During cancer treatment, rehabilitation strategies should focus on restoring occupational performance to levels as close as possible to that present prior to the beginning of the disease process, enabling the woman to preserve her occupational life, consequently resulting in an improvement in HRQoL.
010 Neuroimaging and CSF findings in autoimmune encephalitis with neuronal cell surface antibodies
Authors: Broadley James , Wesselingh Robb , Beech Paul , Seneviratne Udaya , Buzzard Katherine , Butzkueven Helmut , O’Brien Terence , Monif Mastura 
Published: July 2019

IntroductionNeuroimaging and CSF analysis compose essential steps in evaluating patients with autoimmune encephalitis (AE). No study has compared the magnitude and prognostic implications of these findings between different subtypes. Herein we examine cases of AE with neuronal cell surface antibodies, and contrast the results of early investigations.MethodsWe performed medical records search from 2008–2018 in 5 Victorian hospitals. Cases of AE were established in accordance with diagnostic criteria by Graus et al.1 Clinical and laboratory data was collected. All neuroimaging was evaluated independently by a neuroradiologist.ResultsWe identified 52 patients with AE with neuronal cell surface antibodies (21 NMDAR, 27 VGKC, 3 AMPAR, 1 GABAb).We found that among patients with anti-LGI1 antibodies the presence of abnormal CSF correlated with increased rates of mortality (50% vs 0%). This effect was largely mediated by CSF lymphocytosis, which was present in 2 patients who both died within 12 months of diagnosis.We found that the development of hippocampal atrophy was more common amongst patients with abnormal MRI findings, but in particular those with changes affecting the medial temporal lobe. This effect was evident both in the cases with anti-VGKC antibodies (60% vs 18%, 75% vs 17%) and the cohort as a whole (56% vs 15%, 83% vs 13%).ConclusionsThis is the first study of its kind in Australia, and identifies some unique correlation between early investigation findings, and the clinical and radiological outcome. A larger cohort is required to determine if these findings are statistically significant.ReferenceGraus F, Titulaer MJ, Balu R, Benseler S, Bien CG, Cellucci T, Cortese I, Dale RC, Gelfand JM, Geschwind M, Glaser CA, Honnorat J, Hoftberger R, Iizuka T, Irani SR, Lancaster E, Leypoldt F, Pruss H, Rae-Grant A, Reindl M, Rosenfeld MR, Rostasy K, Saiz A, Venkatesan A, Vincent A, Wandinger KP, Waters P, Dalmau J. A clinical approach to diagnosis of autoimmune encephalitis. Lancet Neurol 2016;15:391–404.
120 Prospective study determining the predictive value of inattention in the evaluation of suspected acute stroke; a territory wide study
Authors: Baird-Gunning Jonathan JD , Zhai Shaun , Jones Brett , Nandal Neha , Das Chandi , Patel Ronak , Gawarikar Yash , Lueck Christian 
Published: July 2019

Introduction25%-30% of patients admitted with acute stroke are stroke mimics. Clinical assessment plays a major role in diagnosis in the hyperacute clinical setting. Identifying physical signs that correctly identify stroke is therefore important. A retrospective study1 suggested that the presence of sensory inattention (or neglect) was seen exclusively in stroke patients, suggesting that inattention might be a reliable discriminator between stroke and mimics. This study aimed to test that hypothesis.MethodsProspective assessment of suspected stroke patients for the presence of neglect (NIHSS definition). Neglect could be visual and/or somatosensory. The presence of neglect was then correlated with eventual diagnosis at 48 hours. Sensitivity, specificity and predictive values were calculated. A post-hoc analysis evaluated the correlation of neglect with large vessel occlusion in patients who underwent angiography.Results115 patients were recruited, 70 ultimately with stroke and 45 with other diagnoses. Neglect was present in 27 patients (of whom 23 had stroke) and absent in 88. This yielded: sensitivity 32.9%, specificity 91.1%, positive predictive value 85.2%, and negative predictive value 41.9%. Two patients with neglect had a diagnosis of functional illness, one a seizure, and one a brain tumour. Neglect was present in 7 out of 8 patients with large vessel occlusion (sensitivity 87.5%) and was absent in all patients who did not have large vessel occlusion on angiogram.ConclusionWhen present, neglect is a strong predictor of organic pathology and large vessel occlusion. However, it is not 100% specific and can be seen in functional presentations.ReferenceGargalas S, Weeks R, Khan-Bourne N, Shotbolt P, Simblett S, Ashraf L, Doyle C, Bancroft V, David AS: Incidence and outcome of functional stroke mimics admitted to a hyperacute stroke unit. J Neurol Neurosurg Psychiatry 2017, 88:2–6.
012 Mechanical thrombectomy in pediatric stroke: systematic review, individual patient-data meta-analysis, and case series
Authors: Bhatia Kartik , Kortman Hans , Blair Chris , Parker Geoffrey , Brunacci David , Ang Tim , Worthington John , Muthusami Prakash , Krings Timo 
Published: July 2019

IntroductionIn adults, there is strong evidence demonstrating the superiority of mechanical thrombectomy (MT) plus intravenous thrombolysis over thrombolysis alone for the treatment of acute ischemic stroke due to large vessel occlusion (LVO). The role of MT in the paediatric stroke population is less clear. Here we present an updated systematic review addressing the use of MT in paediatric patients, including three cases from our centre in Sydney, Australia. We have also completed an individual participant data (IPD) meta-analysis of clinical and angiographic outcomes based on these results.MethodOur systematic review and IPD meta-analysis was performed according to PRISMA-IPD (Preferred Reporting Items for Systematic Reviews and Meta-Analyses: Individual Participant Data) guidelines. Primary outcomes measures were change in NIHSS (National Institute of Health Stroke Scale) score following MT, and mRS (modified Rankin Scale) score at final reported follow-up. The secondary outcome measure was final angiographic result using the mTICI (modified Treatment in Cerebral Ischemia) scale.ResultsMT resulted in good long-term neurological outcomes (mRS 0–2) in 60/67 cases (89.6%;follow-up timing µ=4.1 months: 95%CI 2.9–5.3), good short-term neurological outcomes (reduction in NIHSS by 8 or more points or post-MT NIHSS of 0–1) in 37/52 cases (71.2%), and successful recanalization (mTICI 2b/3) in 57/67 cases (85.1%).ConclusionsIn paediatric patients, MT is an effective treatment for ischaemic stroke due to LVO. In the absence of a dedicated prospective registry and with randomized control trails unfeasible, this report represents the best available evidence for the use of MT in the paediatric setting.