bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbial Dynamics during Industrial Rearing, Processing, and Storage of Tropical House Crickets (Gryllodes sigillatus) for Human Consumption
Authors: Vandeweyer Dries , Wynants Enya , Crauwels Sam , Verreth Christel , Viaene Nikolaas , Claes Johan , Lievens Bart , Van Campenhout Leen 
Published: April 2018

ABSTRACTIn this study, the microbiota during industrial rearing, processing, and storage of the edible tropical house cricket,Gryllodessigillatus, was investigated. To this end, we analyzed samples from the cricket feed, obtained before feeding as well as from the cages, and from the crickets during rearing, after harvest, and after processing into frozen, oven-dried, and smoked and oven-dried (smoked/dried) end products. Although the feed contained lower microbial numbers than the crickets, both were dominated by the same species-level operational taxonomic units, as determined by Illumina MiSeq sequencing. They corresponded, among others, to members ofPorphyromonadaceae,Fusobacterium,Parabacteroides, andErwinia. The harvested crickets contained high microbial numbers, but none of the investigated food pathogensSalmonellaspp.,Listeria monocytogenes,Bacillus cereus, or coagulase-positive staphylococci. However, some possible mycotoxin-producing fungi were isolated from the crickets. A postharvest heat treatment, shortly boiling the crickets, reduced microbial numbers, but an endospore load of 2.4 log CFU/g remained. After processing, an increase in microbial counts was observed for the dried and smoked/dried crickets. Additionally, in the smoked/dried crickets, a high abundance of aBacillussp. was observed. Considering the possible occurrence of food-pathogenic species from this genus, it is advised to apply a heat treatment which is sufficient to eliminate spores. Nevertheless, the microbial numbers remained constant over a 6-month storage period, whether frozen (frozen end product) or at ambient temperature (oven-dried and smoked/dried end products).IMPORTANCEThe need for sustainable protein sources has led to the emergence of a new food sector, producing and processing edible insects into foods. However, insight into the microbial quality of this new food and into the microbial dynamics during rearing, processing, and storage of edible insects is still limited. Samples monitored for their microbiota were obtained in this study from an industrial rearing and processing cycle. The results lead first to the identification of process steps which are critical for microbial food safety. Second, they can be used in the construction of a Hazard Analysis and Critical Control Points (HACCP) plan and of a Novel Food dossier, which is required in Europe for edible insects. Finally, they confirm the shelf-life period which was determined by the rearer.
Simian Immunodeficiency Virus Vif and Human APOBEC3B Interactions Resemble Those between HIV-1 Vif and Human APOBEC3G
Authors: Wang Jiayi , Shaban Nadine M. , Land Allison M. , Brown William L. , Harris Reuben S. 
Published: April 2018

ABSTRACT

Several members of the APOBEC3 DNA cytosine deaminase family can potently inhibit Vif-deficient human immunodeficiency virus type 1 (HIV-1) by catalyzing cytosine deamination in viral cDNA and impeding reverse transcription. HIV-1 counteracts restriction with the virally encoded Vif protein, which targets relevant APOBEC3 proteins for proteasomal degradation. HIV-1 Vif is optimized for degrading the restrictive human APOBEC3 repertoire, and, in general, lentiviral Vif proteins specifically target the restricting APOBEC3 enzymes of each host species. However, simian immunodeficiency virus SIV
mac239
Vif elicits a curiously wide range of APOBEC3 degradation capabilities that include degradation of several human APOBEC3s and even human APOBEC3B, a non-HIV-1-restricting APOBEC3 enzyme. To better understand the molecular determinants of the interaction between SIV
mac239
Vif and human APOBEC3B, we analyzed an extensive series of mutants. We found that SIV
mac239
Vif interacts with the N-terminal domain of human APOBEC3B and, interestingly, that this occurs within a structural region homologous to the HIV-1 Vif interaction surface of human APOBEC3G. An alanine scan of SIV
mac239
Vif revealed several residues required for human APOBEC3B degradation activity. These residues overlap HIV-1 Vif surface residues that interact with human APOBEC3G and are distinct from those that engage APOBEC3F or APOBEC3H. Overall, these studies indicate that the molecular determinants of the functional interaction between human APOBEC3B and SIV
mac239
Vif resemble those between human APOBEC3G and HIV-1 Vif. These studies contribute to the growing knowledge of the APOBEC-Vif interaction and may help guide future efforts to disrupt this interaction as an antiviral therapy or exploit the interaction as a novel strategy to inhibit APOBEC3B-dependent tumor evolution.

IMPORTANCE
Primate APOBEC3 proteins provide innate immunity against retroviruses such as HIV and SIV. HIV-1, the primary cause of AIDS, utilizes its Vif protein to specifically counteract restrictive human APOBEC3 enzymes. SIV
mac239
Vif exhibits a much wider range of anti-APOBEC3 activities that includes several rhesus macaque enzymes and extends to multiple proteins in the human APOBEC3 repertoire, including APOBEC3B. Understanding the molecular determinants of the interaction between SIV
mac239
Vif and human APOBEC3B adds to existing knowledge on the APOBEC3-Vif interaction and has potential to shed light on what processes may have shaped Vif functionality over evolutionary time. An intimate understanding of this interaction may also lead to a novel cancer therapy because, for instance, creating a derivative of SIV
mac239
Vif that specifically targets human APOBEC3B could be used to suppress tumor genomic DNA mutagenesis by this enzyme, slow ongoing tumor evolution, and help prevent poor clinical outcomes.
Seasonal temperature variability and emergency hospital admissions for respiratory diseases: a population-based cohort study
Authors: Sun Shengzhi , Laden Francine , Hart Jaime E , Qiu Hong , Wang Yan , Wong Chit Ming , Lee Ruby Siu-yin , Tian Linwei 
Published: April 2018

BackgroundClimate change increases global mean temperature and changes short-term (eg, diurnal) and long-term (eg, intraseasonal) temperature variability. Numerous studies have shown that mean temperature and short-term temperature variability are both associated with increased respiratory morbidity or mortality. However, data on the impact of long-term temperature variability are sparse.ObjectiveWe aimed to assess the association of intraseasonal temperature variability with respiratory disease hospitalisations among elders.MethodsWe ascertained the first occurrence of emergency hospital admissions for respiratory diseases in a prospective Chinese elderly cohort of 66 820 older people (≥65 years) with 10–13 years of follow-up. We used an ordinary kriging method based on 22 weather monitoring stations in Hong Kong to spatially interpolate daily ambient temperature for each participant’s residential address. Seasonal temperature variability was defined as the SD of daily mean summer (June–August) or winter (December–February) temperatures. We applied Cox proportional hazards regression with time-varying exposure of seasonal temperature variability to respiratory admissions.ResultsDuring the follow-up time, we ascertained 12 689 cases of incident respiratory diseases, of which 6672 were pneumonia and 3075 were COPD. The HRs per 1°C increase in wintertime temperature variability were 1.20 (95% CI 1.08 to 1.32), 1.15 (1.01 to 1.31) and 1.41 (1.15 to 1.71) for total respiratory diseases, pneumonia and COPD, respectively. The associations were not statistically significant for summertime temperature variability.ConclusionWintertime temperature variability was associated with higher risk of incident respiratory diseases.
Effect of Storage Containers on Quality of Household Drinking Water in Urban Communities in Ibadan, Nigeria
Authors: Onigbogi Olanrewaju , Ogunyemi Oluwatoyin 
Published: April 2018

<p>The method of storage is essential in maintaining water purity and safety for drinking purposes. This study assessed the effect of various storage containers on household drinking water quality in a resource-limited setting. A quasi-experimental design was adopted. Four communities using protected springs as household drinking water sources were purposively selected. Forty-four households were selected and randomly assigned to four treatment groups; namely Covered Buckets with Taps (CBT), Covered Buckets without Tap (CB), Covered Kegs with Taps (CKT) and Covered Kegs without Tap (CK). Physicochemical analysis and bacteriological analysis were carried out on the water samples before and after they were put in the containers. Data was analyzed using SPSS version 12. At baseline, mean pH values of water samples from the springs and RSC were 6.4±0.6 and 7.1±0.7 respectively which were above the recommended. Mean chloride concentration of springs (14.3±7.4mg/l) and RSC (19.3±10.0 mg/l) samples were below recommended. Mean Total Coliform Count (TCC) of the springs in the four communities was 18.0±4.0 and mean TCC of RSC was 12.7±4.9. Five percent of water samples from RSC had mean <em>E.coli</em> count of 100/100ml. The mean TCC after introducing CB, CBT, CK and CKT in all the communities were 10.0±4.0, 8.5±4.2, 6.9±2.8 and 7.3±3.7 respectively (p&lt;0.05). The use of covered kegs without tap was best in reducing contaminants in drinking water. Education on appropriate household drinking water storage and handling practices is recommended.</p>
Developing Comprehensive Diabetes Education Materials for Structured Patient Education Programs in Primary Care Setting
Authors: Hilman Agrimon Oryzati , Street Jackie 
Published: April 2018

Diabetes education is a key factor for a succesful diabetes care. Comprehensive diabetes education materials for conducting stuctured diabetes education programs were rarely found in primary care setting in Indonesia. There was a need for developing new, comprehensive diabetes education materials for low-literate readers. Developing these education materials followed standard steps in developing print materials, and took account tips for writing low literacy materials for poor readers. The new diabetes education materials consisted of tenvarious leaflets, also printed as14 posters and 14 x-banners. The ten diabetes leaflets were pre-tested to 5 people with type 2 diabetes (T2D). After minor revisions, the leaflets were printed and distributed to 88 people with T2D attending two structured diabetes education programs in Yogyakarta City. These 88 people were requested to evaluate the leaflets using an evaluation form consisting of four items on language usage, font size, use of pictures, and diabetes informationwith a 1-10 rating scale; and an open-ended question for improvement. Descriptive statistics were used to analyze the results<strong>. </strong>Most participants thought that the leaflets were easy to understand and read, interesting, and simple. Majority of participants (79.7%) gave favorable comments without providing suggestions for improvement, such as: “The diabetes leaflets are already good and easy to understand”<em>. </em>One third of the participants gave suggestions for improvement. The comprehensive diabetes leaflets developed were well received and highly appreciated by people with T2D attending diabetes education programs.
Investigations of latrine coverage and associated factors among Debretabor town, Amhara Region North west Ethiopia
Authors: Tafere Yilkal , Woldie Mirkuzie , Assefa Henok 
Published: April 2018

<span style="color: #000000; font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 10px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">Background: Studies in a number of countries have shown that wherever inappropraite waste disposal morbidity and mortality rates are high. Although latrine coverage is an important indicator for measuring success of the health service program; data on latrine coverage scarce in the study area. Objective: To investigate latrine coverage and associated factors in among households of Debretabor town, Amhara region, Ethiopia.<span> </span></span><span style="color: #000000; font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 10px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: #ffffff; text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none;">Methods: A community based cross sectional study was conducted in Debretabor town from September 1-30, 2013. A total of 422 households were included in the study using systematic sampling technique. Data were collected using structured questionnaire and analyzed using SPSS version 16.0. Degree of association between independent and dependent variables was assessed with a 95% confidence level and p-value less than 0.05 was used to detect statistical significance. Result: In this study. Ninety three point five percent of households have latrine. Graduated as model family was predictor for availability of latrine (AOR= 3.18,). Income and house ownership were also found to be predictors for availability of latrine (AOR=3.70), (AOR=8.46), respectively. Educational status of respondents was found to be predictors for availability of latrine (AOR= 2.65). Conclusion: latrine coverage was relatively lower from the national target of 100%, still there are households that use open defication. Educational status, house owner shipe, income and graduated as model family were main factors affecting latrine availability. Improving socio economic status of households, provision of continuous advice and technical support at household level on the availability of latrine are recommended.</span>
Periodontal Disease on Pregnant Women (Case Study: Pondok Labu Village, South Jakarta, Indonesia)
Authors: Rikawarastuti Rikawarastuti , Ngatemi Ngatemi , Harahap Nita Noviani 
Published: April 2018

During preganancy, women periodically concern about ANC due to fetus growth, but ignore their own periodontal health. This research was conducted to acknowledge the effect of age, educational background, parity, body mass index (BMI), diabetes mellitus, smoking habit and oral hygiene with periodontal disease on pregnant women. Analytical observation study with cross sectional design was conducted to pregnant women in Pondok Labu Village<em> (Kelurahan)</em>, South Jakarta. Purposive sampling was performed among 101 respondents. Chi Square and logistic regression were used for the analysis. Results shown that periodontal disease on pregnant women were 73.2% (66.3% were calculus and 6.9% were having periodontal pocket of 4-5 mm). It is proven that there is relationship between age, educational background, parity, BMI, diabetes mellitus, and smoking habit to the periodontal disease. On the other hand, oral hygiene highly affected the periodontal disease on pregnant woman. Poor oral hygiene is the dominant factor of periodontal disease on pregnant women after being controlled by age (OR= 21.33, p value 0.005). It is advised for pregnant women to improve their knowledge on how to care for their oral hygiene prior to and during pregnancy, as well as, go through treatments with dentists.
Knowledge and Attitude of Students on Antimicrobial Resistance at Debre Markos University, Ethiopia
Authors: Zelellw Desalegne Amare , Bizuayehu Habtamu Mellie 
Published: April 2018

Irrational use of medicines is a key reason for the increase and spread of antimicrobial resistance and it is a global concern. It is a fast mounting universal crisis. The main of this study of this study was to assess knowledge, attitude and practice of university students on antimicrobial resistance at Debre Markos University. Institutional based comparative cross-sectional survey was conducted from June to July with total sample size of 670. Participants were selected using simple random sampling method using random number table. Data clerk double entered the data into Epi data version 3.1 and transferred to SPSS Windows software version 21.0. Those variables that had associations at binary stage with p-value &lt; 0.1, not collinear and biologically important variables were entered in to multiple logistic regression models. The cut off point for association was p-value &lt; 0.05.<strong> </strong>About 14.8% study participants had adequate knowledge towards drug resistance. Rural residences of the participants were significantly associated with drug resistance as compared to urban residence. Knowledgeable participants were significantly protective to self- medication and drug resistance as compare to those who had inadequate knowledge and participants who had positive attitude were positively associated with self-medication as compare to those who had negative attitude. The overall knowledge of participants regarding to antimicrobial resistance was low. In the other hand, majority of participants had positive attitude. Those participants who had positive attitude were significantly associated with antimicrobial resistance as compared to who had negative attitude.
Ethnicity, Obesity, and Type 2 Diabetes of Adults in Urban Populations of Central America
Authors: Wuenstel William G , Johnson James A. , Humphries James , Samuel Cheryl 
Published: April 2018

The purpose of this meta-analysis was to examine the impact of ethnicity and obesity as it relates to Type-2 Diabetes (T2D) in specific Central American countries. A meta-analysis was conducted to determine the association of ethnicity, obesity, and T2D. Four studies that qualified for inclusion were identified by searching MEDLINE and PubMed databases. The studies on the association of ethnicity and T2D had a combined population resulted in 265,858 study participants. Two studies on the association of obesity and T2D had 197,899 participants. An analysis of the data was conducted utilizing the relative risk ration, odds ratio, and forest plots. The comparison of the relative risk of T2D across ethnic categories by studies range for Blacks was 1.59 to 2.74, Asians was 1.43 to 2.08, and Hispanics .92 to 2.91. The ethnic difference in the prevalence of diabetes was almost two-fold higher in all ethnic groups than among the Caucasians with a significance level of 95%. A comparison of relative risk of T2D across weight categories was significantly higher among those with a diagnosed of diabetes in all reported areas. The odds ratio was very close to the risk ratio in both ethnicity and obesity to the development of T2D.The meta-analysis findings documented that an association does exist between ethnicity and obesity to the development of type 2 diabetes.
Supplementation of Vitamin A and D in the Medication of Lung Tuberculosis
Authors: Pratiwi Rita Dian , Pramono Dibyo , Iswanto Iswanto , Junaedi Junaedi 
Published: April 2018

<p><span>At District of Wonosobo</span><span lang="EN-US">, Indonesia</span><span> incidence rate of lung </span><span lang="EN-US">tuberculosis</span><span lang="EN-US">(</span><span>TB</span><span lang="EN-US">)</span><span> is increasing over years. In 2011 sputum conversion rate had reached 83.8%. Incidence rate of lung TB is still relatively high. This is influenced by many factors, one of which is the process of transmission. Potential of transmission may still occur until end of intensive medication (2 months). Vitamin A and D as <em>immunoprotection</em> can be used as supplements that can accelerate sputum conversion. To find out effect of vitamin A and D supplementation in accelerating sputum conversion of lung TB patients during intensive phase.<strong> </strong>The study was true experimental <em>(</em>double blinded randomized controlled trial).<em> </em>Subject of the study consisted of two groups; one comprised as many as 30 positive acid fast bacillus lung TB patients supplemented with vitamin A at dosage of 1 x 5000 IU/day and vitamin D at dosage of 1 x 400 IU/day whereas another group was placebo during intensive phase. There was effect of vitamin A and D supplementation to acceleration of sputum conversion with score of p 0.003; HR 2.45. Sputum conversion acceleration occurred in the experiment group during the second week with vitamin A and D supplementation as much as 86%. Vitamin A and D supplementation could accelerate the incidence of sputum conversion as much as 2.45 times greater than placebo group; thus vitamin A and D could be used as complementary supplements in the medication of lung TB medication during intensive phase.</span></p>
Learning Divisions of Fractions through Sprint Running Pictures
Authors: Roni Abdul , Zulkardi Z. , Indra Putri Ratu Ilma 
Published: April 2018

This study aims to produce Learning Trajectory (LT) that can help students understand the division of fractions in grade 5 elementary schools. This study was conducted on 30 students in class V.e MIN 2 Palembang, South Sumatra Province, Indonesia. This study uses Design Research method with three research stages, namely: 1) Preliminary Design/ preparing for an experiment which is the stage of designing Hyphotecal Learning Trajectory (HLT) and instruments needed, 2) Design Experiment namely HLT test phase consisting of Pilot Experiment and Teaching Experiment and 3) Retrospective Analysis. Instruments for data collections are video recordings, documentation, and interviews, observation sheet, and pre-test and post-test. Hypothetical Learning Trajectory (HLT) of learning division of fractions has been designed into Learning Trajectory (LT) so it can function as a Local Instructional Theory (LIT) which can be developed to provide the materials of the division of fractions for students. The results of this study indicate that the trajectory of learning produced can help students understand the division of fractions. Therefore, it will be able to contribute positively to the various parties who have an interest in the education such as teachers, students, schools and the government. Furthermore, it is also can be used as a reference in the teaching and learning and developing materials of teaching mathematics.
Design of Personalized Blended Learning Environments Based On Web-Assisted Modelling In Science Education
Authors: Çetinkaya Murat 
Published: April 2018

<span lang="EN-US">Positive results of science teaching studies supported with the means provided by technology require the enrichment of the content of blended learning environments to provide more benefits. Within this context, it is thought that preparing a web-assisted model-based teaching, which is frequently used in science teaching, based on the “Matter and Heat” unit will be useful. A great number of studies on “Matter and Heat” unit have reported that many students have conceptual errors. It has been found that students have difficulties in associating concepts such as matter, heat, temperature and change of state with daily life. Studies have shown the necessity of designing and using effective teaching methods in solving such problems. It is thought that the modeling based web materials developed for the unit of matter and heat will be effective in decreasing students’ conceptual errors and their learning difficulties. ASSURE teaching design model, which aims to increase the efficiency of material choice and use with systematic prior planning of teaching, was used in creating the materials which were prepared based on web-assisted modeling. The purpose of this study is to design of personalized blended learning environments which are designed based on web-assisted modeling for “Matter and Heat” unit. </span>