bluuebunny/crossref_metadata_2025_split huggingface dataset:
MEG3 inhibits proliferation and promote apoptosis in osteoarthritis chondrocytes by miR-361-5p/wnt/β-catenin axis
Authors: Wang Anying , Hu Naixia , Zhang Yefeng , Chen Yuanzhen , Su Changhui , Lv Yao , Shen Yong 
Published: July 2019

Abstract
Background: This study aimed to investigate the role of long noncoding RNA (lncRNA) maternally expressed 3 (MEG3) and related molecular mechanisms in osteoarthritis (OA).
Methods: Patients with OA and patients undergoing thigh amputation were involved in OA group and control group, respectively. Cartilage tissues of all patients were isolated and cultured. Based on different transfection, MEG3 cells were grouped into Blank, pcDNA3.1-NC, pcDNA3.1-MEG3, si-NC, si-MEG3, pcDNA3.1-NC + mimics NC, pcDNA3.1-MEG3 + mimics NC, pcDNA3.1-NC + miR-361-5p mimics and pcDNA3.1-MEG3 + miR-361-5p mimics group. The cells transfected with pcDNA3.1-NC and pcDNA3.1-MEG3, and then cultured with XAV939 was named as pcDNA3.1-NC +XAV939 group and pcDNA3.1-MEG3 + XAV939 group respectively. The RT-qPCR was used to detect the expression of MEG3 and miR-361-5p. Moreover, Western blot, luciferase reporter assay, RIP, CCK-8 and flow cytometry analysis were performed to reveal the morphology, proliferation and apoptosis in cartilage cells. Finally, the histological analysis and immunostaining were performed on OA rat model.
Results: The expression of lncRNA MEG3 and miR-361-5p in OA was significantly decreased and increased respectively than that in normal. Meanwhile, MEG3 was competitive binding with miR-361-5p in OA chondrocytes. Moreover, the Western blot and CCK-8 assay showed that MEG3 might inhibit cell proliferation and promote cell apoptosis via Wnt/β-catenin pathway. Finally, rat model analysis showed that MEG3 contributed to the cartilage matrix degradation.
Conclusion: MEG3 and miR-361-5p might down-regulated and up-regulated respectively in the chondrocytes of OA patients. Furthermore, MEG3 might inhibit cell proliferation and promote cell apoptosis via miR-361-5p/Wnt/β-catenin axis in OA chondrocytes.
A lil3 chl p double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice
Authors: Li Chunmei , Liu Xin , Pan Jihong , Guo Jia , Wang Qian , Chen Congping , Li Na , Zhang Kuan , Yang Bin , Sun Changhui , Deng Xiaojian , Wang Pingrong 
Published: July 2019

Abstract
Background:
Phytyl residues are the common side chains of chlorophyll (Chl) and tocopherols. Geranylgeranyl reductase (GGR), which is encoded by CHL P gene, is responsible for phytyl biosynthesis. The light-harvesting like protein LIL3 was suggested to be required for stability of GGR and protochlorophyllide oxidoreductase in Arabidopsis.
Results:
In this study, we isolated a yellow-green leaf mutant, 637ys, in rice (Oryza sativa). The mutant accumulated majority of Chls with unsaturated geranylgeraniol side chains and displayed a yellow-green leaf phenotype through the whole growth period. The development of chloroplasts was suppressed, and the major agronomic traits, especially No. of productive panicles per plant and of spikelets per panicle, dramatically decreased in 637ys. Besides, the mutant exhibited to be sensitive to light intensity and deficiency of tocopherols without obvious alteration in tocotrienols in leaves and grains. Map-based cloning and complementation experiment demonstrated that a point mutation on OsLIL3 gene accounted for the mutant phenotype of 637ys. OsLIL3 is mainly expressed in green tissues and its encoded protein is targeted to the chloroplast. Furthermore, the 637ys 502ys (lil3 chl p) double mutant exclusively accumulated geranylgeranyl Chl and exhibited lethality at the three-leaf stage.
Conclusions:
Taken together, we succeeded to identify OsLIL3 gene through a map-based cloning approach. Meanwhile, we demonstrated that OsLIL3 is of extreme importance to the function of OsGGR, and that the complete replacement of phytyl side chain of chlorophyll by geranylgeranyl chain could be fatal to plant survival in rice.
Distinct molecular etiologies of male and female hepatocellular carcinoma
Authors: Natri Heini Maaret , Wilson Melissa A , Buetow Kenneth H 
Published: July 2019

Abstract
Sex-differences in cancer occurrence and mortality are evident across tumor types; men exhibit higher rates of incidence and often poorer responses to treatment. Targeted approaches to the treatment of tumors that account for these sex-differences require the characterization and understanding of the fundamental biological mechanisms that differentiate them. Hepatocellular Carcinoma (HCC) is the second leading cause of cancer death worldwide, with the incidence rapidly rising. HCC exhibits a male-bias in occurrence and mortality, but previous studies have failed to explore the sex-specific dysregulation of gene expression in HCC. Here, we characterize the sex-shared and sex-specific regulatory changes in HCC tumors in the TCGA LIHC cohort. By using a sex-specific differential expression analysis of tumor and tumor-adjacent samples, we uncovered etiologically relevant genes and pathways differentiating male and female HCC. While both sexes exhibited activation of pathways related to apoptosis and cell cycle, males and females differed in the activation of several signaling pathways, with females showing PPAR pathway enrichment while males showed PI3K, 305 PI3K/AKT, FGFR, EGFR, NGF, GF1R, Rap1, DAP12, and IL-2 signaling pathway enrichment. Using eQTL analyses, we discovered germline variants with differential effects on tumor gene expression between the sexes. 24.3% of the discovered eQTLs exhibit differential effects between the sexes, illustrating the substantial role of sex in modifying the effects of eQTLs in HCC. The genes that showed sex-specific dysregulation in tumors and those that harbored a sex-specific eQTL converge in clinically relevant pathways, suggesting that the molecular etiologies of male and female HCC are partially driven by differential genetic effects on gene expression. Overall, our results provide new insight into the role of inherited genetic regulation of transcription in modulating sex-differences in HCC etiology and provide a framework for future studies on sex-biased cancers.
Detecting adverse events in clinical care using natural language processing
Authors: Klein Dorthe , Rennenberg R.J.M.W. , Heuvel F.A.G. van den , Koopmans R.P. , Prins M.H. 
Published: July 2019

Abstract
Background
Current methods for retrospective review of medical records require both time- and cost-wise a substantial effort. Therefore, we wanted to find the best method (based on natural language processing (NLP)) to select cases out of the medical records for further investigation in search for a (potentially preventable) adverse event (AE) to the decrease this effort.
Methods
The basic dataset consisted of 2987 medical records of patients who died during their hospitalization. To gain insight into the signal to noise ratio of the various resources, several subsets of our basic dataset were tested. Thereafter, we tested the scalability. After the best subset was chosen, several NLP algorithms were tested to select the best performing algorithm for the detecting of AEs. In the last experiment we tested the performance of the computer algorithms to predict potentially preventable AEs. The results of the NLP were compared with the outcome of the original retrospective medical record review.
Results
The dataset which contained he last three letters of the medical record showed the biggest potential. The scalability experiment showed that more data leads to a better performance of the algorithm. The best performing algorithm in the third test was the one based on support vector machine (SVM), with a precision of 79%, a negative predictive value (NPV) of 95% and a specificity of 85%. The results of the preventability experiment showed that the performance of the algorithms was almost equal to the results of the AEs.
Conclusions
In this study, we have shown that the SVM algorithm generates the most accurate results for the selection of cases for further investigation in the search for a (potentially preventable) AE. The sensitivity of the algorithms was around 75%. However, the SVM algorithm selected fewer cases to be examined for AEs compared to the original method. Consequently, this would lead to a lower workload for the committee. At the same time, there are a substantial number of cases, with potentially preventable AEs, not detected by machine learning.
Development of a targeted client communication intervention to women using an electronic maternal and child health registry: a qualitative study
Authors: Bogale Binyam , Mørkrid Kjersti , O'Donnell Brian , Ghanem Buthaina , Ward Itimad Abu , Khader Khadija Abu , Isbeih Mervett , Frost Michael , Baniode Mohammad , Hijaz Taghreed , Awwad Tamara , Rabah Yousef , Frøen Frederik 
Published: July 2019

Abstract
Background

Targeted client communication (TCC) using text messages can inform, motivate and remind pregnant and postpartum women of timely utilization of care. The mixed results of the effectiveness of TCC interventions points to the importance of theory based co-design with users. Our aim is to describe the planning, development, and evaluation of a theory based TCC intervention, tailored to pregnant and postpartum women, and automated from an electronic maternal and child health registry in Palestine.

Methods

We used the Health Belief Model to develop interview guides to explore women’s perceptions of antenatal care (ANC), with a focus on high-risk pregnancy conditions (anemia, hypertensive disorders in pregnancy, gestational diabetes mellitus, and fetal growth restriction) and timely ANC attendance, issues predefined by a national expert panel as being of high interest. We performed 18 in-depth interviews with women and eight with healthcare providers in public primary healthcare clinics in the West Bank and Gaza. Following the results from the Health Belief Model, we used concepts from the Model of Actionable Feedback, social nudging and Enhanced Active Choice to compose the TCC content to be sent via SMS. We assessed the acceptability and understandability of the draft text messages through unstructured interviews with local health promotion experts, healthcare providers, and pregnant women.

Results

We found low awareness of the importance of timeliness in attending ANC and the benefits of ANC for pregnancy outcomes. We identified knowledge gaps and beliefs in the domains of low awareness of susceptibility to, and severity of, such complications in pregnancy. We composed actionable text messages following recommended message framing models and iteratively improved the contents with users to bridge the identified gaps. We developed algorithms to trigger tailored text messages with higher intensity for women with a higher risk profile.

Conclusions:
We developed an optimized text message based TCC intervention underpinned by behavior change theories and co-designed with users following an iterative process. The effectiveness of the TCC intervention is assessed in a cluster-randomized trial (ISRCTN10520687).
PREVALÃŠNCIA DE PEDICULOSE EM ESTUDANTES DO MUNICÃPIO DE NOVA IGUAÃ‡U, ESTADO DO RIO DE JANEIRO, BRASIL
Authors: De Oliveira AndrÃ© Fernandez , Norberg Antonio Neres , De Oliveira JosÃ© Tadeu Madeira , Ribeiro Paulo CÃ©sar , Norberg Paulo Roberto Blanco Moreira , Freire Nicolau MauÃ©s Serra 
Published: July 2019

A pediculose por Pediculus humanus capitis Ã© uma infestaÃ§Ã£o de distribuiÃ§Ã£o cosmopolita, em qualquer idade, mas prevalente em crianÃ§as, e se constitui um dos sÃ©rios problemas de saÃºde pÃºblica na atualidade. Objetivo: Avaliar a prevalÃªncia de pediculose em escolares da rede pÃºblica de ensino da cidade de Nova IguaÃ§u, estado do Rio de Janeiro. Pacientes e mÃ©todos: Estudo descritivo, transversal, realizado com 10.851 crianÃ§as com idades entre 4 e 12 anos, matriculadas em 72 escolas do municÃ­pio de Nova IguaÃ§u. A avaliaÃ§Ã£o consistiu na inspeÃ§Ã£o visual e palpaÃ§Ã£o das cabeÃ§as por dois minutos, com atenÃ§Ã£o Ã nuca, pescoÃ§o e regiÃ£o retroauricular, com a manipulaÃ§Ã£o do cabelo. O material encontrado foi identificado no LaboratÃ³rio de Parasitologia da Universidade IguaÃ§u. Resultados: Dos 10.851 estudantes examinados, 4.765 eram meninos e 6.086 eram meninas; do total, 4.023 estavam positivos para pediculose, correspondendo ao coeficiente de prevalÃªncia de 37,07%. A cor do cabelo influencia o parasitismo, mas o tipo de cabelo nÃ£o. O comprimento do cabelo sÃ³ influenciou no parasitismo dos meninos. ConclusÃµes: A pediculose capitis constitui um importante problema de saÃºde pÃºblica entre estudantes do ensino fundamental, e deve ser integrada em programas de prevenÃ§Ã£o e controle de doenÃ§as infecto-parasitÃ¡rias.
DA OCUPAÃ‡ÃƒO DOS LUGARES SINTÃTICOS DE SUJEITO E OBJETO - RETROSPECTIVAS
Authors: Buthers Christiane Miranda 
Published: July 2019

Este artigo objetiva realizar uma retrospectiva dos aspectos semÃ¢ntico-sintÃ¡ticos relacionados Ã ocupaÃ§Ã£o dos lugares sintÃ¡ticos de sujeito e objeto. AtravÃ©s da anÃ¡lise de textos de vÃ¡rios autores, objetiva-se chegar a uma constataÃ§Ã£o de que nÃ£o apenas aspectos relacionados Ã sintaxe influenciam na localizaÃ§Ã£o e identificaÃ§Ã£o do sujeito, como tambÃ©m fatores relacionados aos modos de enunciaÃ§Ã£o desempenham um papel considerado fundamental. Conclui-se que Ã© de extrema importÃ¢ncia o poder da enunciaÃ§Ã£o no trabalho de investigaÃ§Ã£o linguÃ­stica, tendo em vista que ela amplia as possibilidades do estabelecimento de uma gramÃ¡tica realmente diferenciada â€“ que considere aspectos exteriores como constitutivos dos fatos gramaticais, pois a grande questÃ£o para a LinguÃ­stica Ã© onde colocar a exterioridade.
VARIABILIDADE ESPACIAL DE ATRIBUTOS QUÃMICOS DE LATOSSOLO AMARELO DISTROCOESO SOB SISTEMA DE PLANTIO DIRETO NO RECÃ”NCAVO DA BAHIA
Authors: Barros TaÃ­za Nogueira , Melo Filho JosÃ© Fernandes , Peixoto Devison Souza , BrandÃ£o Victor Ferreira , Queiroz Maria Gabriela de , Machado Wilber Pereira 
Published: July 2019

O estudo da variabilidade espacial dos atributos quÃ­micos dos solos Ã© particularmente de grande importÃ¢ncia em Ã¡reas onde o solo estÃ¡ sob plantio direto. Sendo assim, este trabalho teve como objetivo avaliar a variabilidade espacial de trÃªs atributos quÃ­micos de um Latossolo Amarelo Distrocoeso, sob sistema de plantio direto. O trabalho foi realizado em campo experimental da Universidade Federal do RecÃ´ncavo da Bahia, localizado no municÃ­pio de Cruz das Almas â€“ BA, o esquema de amostragem utilizado foi um â€œgridâ€ formado pela interseÃ§Ã£o de 50 pontos, espaÃ§ados em intervalos regulares de 2 m, em cada ponto foram coletadas amostras deformadas, utilizadas para avaliar os seguintes atributos: pH em Ã¡gua (acidez efetiva), CTCefetiva e matÃ©ria orgÃ¢nica do solo. Os atributos quÃ­micos do solo foram avaliados por meio da estatÃ­stica descritiva e de tÃ©cnicas geoestatÃ­sticas. A acidez ativa do solo (pH em Ã¡gua) apresentou distribuiÃ§Ã£o normal e o coeficiente de variaÃ§Ã£o foi classificados como baixo. O modelo gaussiano foi o modelo que melhor ajustou-se Ã acidez ativa do solo. A CTCefetiva apresentou distribuiÃ§Ã£o normal, o coeficiente de variaÃ§Ã£o foi baixo e ajustou-se melhor ao modelo gaussiano apresentando forte grau de dependÃªncia espacial. A M.O.S. apresentou distribuiÃ§Ã£o normal, o CV foi classificado como baixo e apresentou efeito pepita puro.
ACOMPANHAMENTO SOCIOFAMILIAR DAS CRIANÃ‡AS E ADOLESCENTES EGRESSOS DA INSTITUIÃ‡ÃƒO DE ACOLHIMENTO CONCRIAD
Authors: Franca Silva Gustavo Thayllon , Ferreira Priscylla de Oliveira Vieira , Souza AndrÃ© Gomes de , Carvalho MÃ¡rcia Helena de 
Published: July 2019

Este estudo teve como principal objetivo demonstrar como a equipe tÃ©cnica dos equipamentos pÃºblicos estatais Centro de ReferÃªncia Especializado de AssistÃªncia Social e Centro de ReferÃªncia de AssistÃªncia Social realiza o acompanhamento sociofamiliar. Para tal foi desenvolvida pesquisa bibliogrÃ¡fica com os principais autores que discutem a temÃ¡tica no Brasil e entrevistas com uma profissional que atua no ConsÃ³rcio Intermunicipal de AtenÃ§Ã£o Ã CrianÃ§a e ao Adolescente, responsÃ¡vel pelo abrigamento institucional na Comarca de Tarumirim, alÃ©m de entrevistas com as 04 profissionais dos equipamentos pÃºblicos de proteÃ§Ã£o social conveniados A pesquisa realizada foi do tipo quali/quantitativa e os dados representados atravÃ©s de grÃ¡ficos com estatÃ­stica simples, a anÃ¡lise realizada utilizou-se como mÃ©todo o crÃ­tico dialÃ©tico. Os resultados obtidos demonstram que embora na maior parte dos casos de abrigamento as famÃ­lias tenham sido acompanhadas realizando um desligamento responsÃ¡vel.
Effects of two typical revegetation methods on soil moisture in the semi-arid Loess Plateau, China
Authors: Zhao Qian , Yang Lei , Wang Xin , Bi Runcheng , Zhang Qindi 
Published: July 2019

Abstract
Understanding the effects of vegetation on soil moisture is vital to the ecosystem restoration in water-restricted areas. For this study, the effects of introduced revegetation and natural revegetation on soil water (0–1.8 m) were investigated in the Chinese Loess Plateau, which was based on an in situ vegetation removal experiment and two years of soil moisture monitoring. The results indicated that under introduced revegetation, pasture grassland had lower soil moisture but higher temporal variations over the growing season. Compared with abandoned farmlands and native grasslands under natural revegetation, pasture grasslands revealed greater negative effects on deep soil moisture (1–1.8 m), which was difficult to recover following soil desiccation. In contrast, for abandoned farmlands and native grasslands, the surface soil moisture (0–0.4 m) was mainly impacted, which was easily replenished through rainfall events. These outcomes implied that natural revegetation, rather than introduced revegetation, should be the first choice in water-limited regions toward the rehabilitation of degraded ecosystems.
Understanding Comorbidities and Their Contribution to Predictors of Medical Resource Utilization for an Age- and Sex-Matched Patient Population Living With HIV: Cross-Sectional Study
Authors: Odlum Michelle , Yoon Sunmoo 
Published: July 2019

BackgroundMore than 60% of people aging with HIV are observed to have multiple comorbidities, which are attributed to a variety of factors (eg, biological and environmental), with sex differences observed. However, understanding these differences and their contribution to medical resource utilization remains challenging as studies conducted exclusively and predominantly among males do not translate well to females, resulting in inconsistent findings across study cohorts and limiting our knowledge of sex-specific comorbidities.ObjectiveThe objective of the study was to provide further insight into aging-related comorbidities, their associated sex-based differences, and their contribution to medical resource utilization, through the analysis of HIV patient data matched by sex.MethodsInternational Classification of Disease 9/10 diagnostic codes that comprise the electronic health records of males (N=229) and females (N=229) were categorized by individual characteristics, chronic and mental health conditions, treatment, high-risk behaviors, and infections and the codes were used as predictors of medical resource utilization represented by Charlson comorbidity scores.ResultsSignificant contributors to high Charlson scores in males were age (beta=2.37; 95% CI 1.45-3.29), longer hospital stay (beta=.046; 95% CI 0.009-0.083), malnutrition (beta=2.96; 95% CI 1.72-4.20), kidney failure (beta=2.23; 95% CI 0.934-3.52), chemotherapy (beta=3.58; 95% CI 2.16-5.002), history of tobacco use (beta=1.40; 95% CI 0.200-2.61), and hepatitis C (beta=1.49; 95% CI 0.181-2.79). Significant contributors to high Charlson scores in females were age (beta=1.37; 95% CI 0.361-2.38), longer hospital stay (beta=.042; 95% CI 0.005-0.078), heart failure (beta=2.41; 95% CI 0.833-3.98), chemotherapy (beta=3.48; 95% CI 1.626-5.33), and substance abuse beta=1.94; 95% CI 0.180, 3.702).ConclusionsOur findings identified sex-based differences in medical resource utilization. These include kidney failure for men and heart failure for women. Increased prevalence of comorbidities in people living long with HIV has the potential to overburden global health systems. The development of narrower HIV phenotypes and aging-related comorbidity phenotypes with greater clinical validity will support intervention efficacy.
QUALITATIVE PHYTOCHEMICAL SCREENING AND FTIR SPECTROSCOPIC ANALYSIS OF GREWIA TILIFOLIA (VAHL) LEAF EXTRACTS
Authors: DEVI DHARMASOTH RAMA , BATTU GANGA RAO 
Published: July 2019

Objective: The present study aim is to analyse the phytochemicals present in Grewia tilifolia (Vahl) leaf extracts by using Qualitative phytochemical analysis, Fourier-transform infrared spectroscopy (FTIR).
Methods: The leaf extracts were prepared using eight different solvents. The phytochemical analysis Fourier transform infrared spectroscopy (FT-IR) analysis were performed using standard methods.
Results: The FTIR spectroscopic studies revealed different characteristic peak values with various functional groups present in the compounds of respective extracts. The FT-IR analysis shows the presence of different functional groups such as alcohols, phenols, alkanes, carboxylic acids, aldehydes, ketones, alkenes, primary amines, aromatics, esters, ethers, alkyl halides and aliphatic amine compounds, which showed major compounds present in the leaf extracts. The present study generated the FTIR spectrum profile for the medicinally important plant Grewia tilifolia.
Conclusion: The present study provides evediences that different extracts of Grewia tilifolia leaf is useful to cure many serious diseases which remained still problematic and for further isolation of bioactive compounds from the plant which could be of interest for the development of the new drug.