bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymeric nanoparticles as an alternative for application of gibberellic acid in sustainable agriculture: a field study
Authors: Pereira Anderson do Espírito Santo , Oliveira Halley Caixeta , Fraceto Leonardo Fernandes 
Published: May 2019

AbstractNanocarrier systems for the encapsulation of agrochemicals can contribute to sustainable agriculture, but few nanosystems have been developed for plant growth regulators (PGRs). The present study evaluated the effects of seed priming using alginate/chitosan (nanoALG/CS) and chitosan/tripolyphosphate (nanoCS/TPP) containing GA3 on the growth and productivity of Solanum lycopersicum cultivated under field conditions. The results demonstrated that nanocarrier systems could improve fruit production, with the productivity increasing almost 4-fold using nanoALG/CS-GA3. This pioneering study demonstrates the potential of nanocarrier systems with PGRs for applications in agriculture.
Community profiling of the intestinal microbial community of juvenile Hammerhead Sharks (Sphyrna lewini) from the Rewa Delta, Fiji
Authors: Juste-Poinapen Natacha M. S. , Yang Lu , Ferreira Marta , Poinapen Johann , Rico Ciro 
Published: May 2019

AbstractFourteen juvenile scalloped hammerhead sharks (Sphyrna lewini; SHS) were captured between November and December 2014 in the Rewa Delta in Fiji, and assessed for intestinal microflora characterisation using 16S rRNA amplicon sequencing by Illumina Miseq. The microbial population revealed a fluctuating dominance between the Enterobacteriaceae and Vibrionaceae families, namely Citrobacter and Photobacterium spp. Other related marine operational taxonomic units were closely related to Afipia felis, Chloroflexus aggregans, Psychrobacter oceani, Pontibacter actiniarum and Shigella sonnei. Two sharks had distinctive profiles that were dominated by known pathogens, namely Aeromonas salmonicida and Klebsiella pneumonia. The presence of a Methanosaeta species, and of Shigella and Psychrobacter, would suggest sewage contamination because of a spill that occurred on the 6th of December 2014. This study successfully establishes a baseline for future research.
Real-world safety and efficacy of paritaprevir/ritonavir/ombitasvir plus dasabuvir ± ribavirin in patients with hepatitis C virus genotype 1 and advanced hepatic fibrosis or compensated cirrhosis: a multicenter pooled analysis
Authors: Chen Chun-Hsien , Chen Chien-Hung , Lin Chih-Lang , Lin Chun-Yen , Hu Tsung-Hui , Tung Shui-Yi , Hsieh Sen-Yung , Lu Sheng-Nan , Chien Rong-Nan , Hung Chao-Hung , Sheen I-Shyan 
Published: May 2019

AbstractParitaprevir/ritonavir, ombitasvir, and dasabuvir (PrOD) with or without ribavirin shows favorable results in hepatitis C virus genotype 1 (HCV-1) patients in terms of safety and efficacy, but real-world data remain limited for those with advanced hepatic fibrosis (fibrosis 3, F3) or compensated cirrhosis (F4). A total of 941 patients treated in four hospitals (the Keelung, the Linkuo, the Chiayi and the Kaohsiung Chang Gung Memorial Hospital) through a nationwide government-funded program in Taiwan were enrolled. Patients with HCV and advanced hepatic fibrosis or compensated cirrhosis received 12 weeks of PrOD in HCV-1b and 12 or 24 weeks of PrOD plus ribavirin therapy in HCV-1a without or with cirrhosis. Advanced hepatic fibrosis or compensated cirrhosis was confirmed by either ultrasonography, fibrosis index based on 4 factors (FIB-4) test, or transient elastography/acoustic radiation force impulse (ARFI). The safety and efficacy (sustained virologic response 12 weeks off therapy, SVR12) were evaluated. An SVR12 was achieved in 887 of 898 (98.8%) patients based on the per-protocol analysis (subjects receiving ≥1 dose of any study medication and HCV RNA data available at post-treatment week 12). Child-Pugh A6 (odds ratio: 0.168; 95% confidence interval (CI): 0.043–0.659, p = 0.011) was the only significant factor of poor SVR12. Fifty-four (5.7%) patients were withdrawn early from the treatment because of hepatic decompensation (n = 18, 1.9%) and other adverse reactions. Multivariate analyses identified old age (odds ratio: 1.062; 95% CI: 1.008–1.119, p = 0.024) and Child-Pugh A6 (odds ratio: 4.957; 95% CI: 1.691–14.528, p = 0.004) were significantly associated with hepatic decompensation. In conclusion, this large real-world cohort proved PrOD with or without ribavirin to be highly effective in chronic hepatitis C patients with advanced hepatic fibrosis or compensated cirrhosis. However, Child-Pugh A6 should be an exclusion criterion for first-line treatment in these patients.
Integration of bilateral nociceptive inputs tunes spinal and cerebral responses
Authors: Rustamov Nabi , Northon Stéphane , Tessier Jessica , Leblond Hugues , Piché Mathieu 
Published: May 2019

AbstractTogether with the nociceptive system, pain protects the body from tissue damage. For instance, when the RIII-reflex is evoked by sural nerve stimulation, nociceptive inputs activate flexor muscles and inhibit extensor muscles of the affected lower limb while producing the opposite effects on the contralateral muscles. But how do the spinal cord and brain integrate concurrent sensorimotor information originating from both limbs? This is critical for evoking coordinated responses to nociceptive stimuli, but has been overlooked. Here we show that the spinal cord integrates spinal inhibitory and descending facilitatory inputs during concurrent bilateral foot stimulation, resulting in facilitation of the RIII-reflex and bilateral flexion. In these conditions, high-gamma oscillation power was also increased in the dorsolateral prefrontal, anterior cingulate and sensorimotor cortex, in accordance with the involvement of these regions in cognitive, motor and pain regulation. We propose that the brain and spinal cord can fine-tune nociceptive and pain responses when nociceptive inputs arise from both lower limbs concurrently, in order to allow adaptable behavioural responses.
Differences in the molecular signatures of mucosal-associated invariant T cells and conventional T cells
Authors: Park Daeui , Kim Hong Gi , Kim Miok , Park Tamina , Ha Hyung-Ho , Lee Dae Ho , Park Kang-Seo , Park Seong Jun , Lim Hwan Jung , Lee Chang Hoon 
Published: May 2019

AbstractMucosal-associated invariant T (MAIT) cells exhibit different characteristics from those of TCRα7.2− conventional T cells. They play important roles in various inflammatory diseases, including rheumatoid arthritis and inflammatory bowel disease. MAIT cells express a single T cell receptor alpha chain, TCRα7.2 segment associated with Jα33 and CDR3 with fixed length, which recognizes bacteria-derived vitamin B metabolites. However, the characteristics of MAIT cells and TCRα7.2+ CD161− T cells have never been compared. Here, we performed RNA sequencing to compare the properties of MAIT cells, TCRα7.2− conventional T cells and TCRα7.2+ CD161− T cells. Genome-wide transcriptomes of MAIT cells, TCRα7.2− conventional T cells, and TCRα7.2+ CD161− T cells were compared and analyzed using causal network analysis. This is the first report comparing the transcriptomes of MAIT cells, TCRα7.2− conventional T cells and TCRα7.2+ CD161− T cells. We also identified the predominant signaling pathways of MAIT cells, which differed from those of TCRα7.2− conventional T cells and TCRα7.2+ CD161− T cells, through a gene set enrichment test and upstream regulator analysis and identified the genes responsible for the characteristic MAIT cell phenotypes. Our study advances the complete understanding of MAIT biology.
GABAergic neurons in the olfactory cortex projecting to the lateral hypothalamus in mice
Authors: Murata Koshi , Kinoshita Tomoki , Fukazawa Yugo , Kobayashi Kenta , Kobayashi Kazuto , Miyamichi Kazunari , Okuno Hiroyuki , Bito Haruhiko , Sakurai Yoshio , Yamaguchi Masahiro , Mori Kensaku , Manabe Hiroyuki 
Published: May 2019

AbstractOlfaction guides goal-directed behaviours including feeding. To investigate how central olfactory neural circuits control feeding behaviour in mice, we performed retrograde tracing from the lateral hypothalamus (LH), an important feeding centre. We observed a cluster of retrogradely labelled cells distributed in the posteroventral region of the olfactory peduncle. Histochemical analyses revealed that the majority of these retrogradely labelled projection neurons expressed glutamic acid decarboxylase 65/67 (GAD65/67), but not vesicular glutamate transporter 1 (VGluT1). We named this region containing GABAergic projection neurons the ventral olfactory nucleus (VON) to differentiate it from the conventional olfactory peduncle. VON neurons were less immunoreactive for DARPP-32, a striatal neuron marker, compared to neurons in the olfactory tubercle and nucleus accumbens, which distinguished the VON from the ventral striatum. Fluorescent labelling confirmed putative synaptic contacts between VON neurons and olfactory bulb projection neurons. Rabies-virus-mediated trans-synaptic labelling revealed that VON neurons received synaptic inputs from the olfactory bulb, other olfactory cortices, horizontal limb of the diagonal band, and prefrontal cortex. Collectively, these results identify novel GABAergic projection neurons in the olfactory cortex that may integrate olfactory sensory and top-down inputs and send inhibitory output to the LH, which may modulate odour-guided LH-related behaviours.
Heterogeneous flow inside threads of low viscosity fluids leads to anomalous long filament lifetimes
Authors: Recktenwald Steffen M. , Haward Simon J. , Shen Amy Q. , Willenbacher Norbert 
Published: May 2019

AbstractFormation and breakup of fluid threads is pervasive in nature and technology, where high extensibility of fluid filaments and extended filament lifetimes are commonly observed as a consequence of fluid viscoelasticity. In contrast, threads of low viscous Newtonian fluids like water rupture quickly. Here, we demonstrate that a unique banding instability during filament thinning of model surfactant solutions, with a viscosity close to water and no measurable elasticity, leads to extremely long filament lifetimes and to the formation of remarkably long threads. Complementary measurements in planar extension as well as in shear reveal that this flow instability is characterized by a multivalued stress, arising beyond a critical strain rate, irrespective of flow kinematics. Our work reports the first observation of such phenomena during extensional deformation and provides a unifying view on instabilities in complex flow fields.
Reconfigurable nanoscale spin-wave directional coupler using spin-orbit torque
Authors: Ren Zhiwei , Liu Shuang , Jin Lichuan , Wen Tianlong , Liao Yulong , Tang Xiaoli , Zhang Huaiwu , Zhong Zhiyong 
Published: May 2019

AbstractWe present a reconfigurable nanoscale spin-wave directional coupler based on spin-orbit torque (SOT). By micromagnetic simulations, it is demonstrated that the functionality and operating frequency of proposed device can be dynamically switched by inverting the whole or part of the relative magnetic configuration of the dipolar-coupled waveguides using SOT. Utilizing the effect of sudden change in coupling length, the functionality of power divider can be realized. The proposed reconfigurable spin-wave directional coupler opens a way for two-dimensional planar magnonic integrated circuits.
Interstitial hydrogen atoms in face-centered cubic iron in the Earth’s core
Authors: Ikuta Daijo , Ohtani Eiji , Sano-Furukawa Asami , Shibazaki Yuki , Terasaki Hidenori , Yuan Liang , Hattori Takanori 
Published: May 2019

AbstractHydrogen is likely one of the light elements in the Earth’s core. Despite its importance, no direct observation has been made of hydrogen in an iron lattice at high pressure. We made the first direct determination of site occupancy and volume of interstitial hydrogen in a face-centered cubic (fcc) iron lattice up to 12 GPa and 1200 K using the in situ neutron diffraction method. The transition temperatures from the body-centered cubic and the double-hexagonal close-packed phases to the fcc phase were higher than reported previously. At pressures <5 GPa, the hydrogen content in the fcc iron hydride lattice (x) was small at x < 0.3, but increased to x > 0.8 with increasing pressure. Hydrogen atoms occupy both octahedral (O) and tetrahedral (T) sites; typically 0.870(±0.047) in O-sites and 0.057(±0.035) in T-sites at 12 GPa and 1200 K. The fcc lattice expanded approximately linearly at a rate of 2.22(±0.36) Å3 per hydrogen atom, which is higher than previously estimated (1.9 Å3/H). The lattice expansion by hydrogen dissolution was negligibly dependent on pressure. The large lattice expansion by interstitial hydrogen reduced the estimated hydrogen content in the Earth’s core that accounted for the density deficit of the core. The revised analyses indicate that whole core may contain hydrogen of 80(±31) times of the ocean mass with 79(±30) and 0.8(±0.3) ocean mass for the outer and inner cores, respectively.
Mutant huntingtin disturbs circadian clock gene expression and sleep patterns in Drosophila
Authors: Faragó Anikó , Zsindely Nóra , Bodai László 
Published: May 2019

AbstractDeficiency of the sleep-wake cycle can accelerate the progression of Huntington’s disease (HD) and exacerbate symptoms making it a target of investigation to better understand the molecular pathology of the disorder. In this study we analyzed sleep defects in a Drosophila model of HD and investigated whether disturbed sleep coincides with alterations in the molecular mechanism controlling circadian rhythm. To analyze sleep defects we recorded the daily activity of flies in 12:12 hours light:dark entrainment and in regard to the underlying molecular mechanism measured circadian “clock” gene expression. In HD flies we observed reduced amount of sleep, sleep fragmentation and prolonged sleep latency. We found changes in gene expression patterns of both transcriptional feedback loops of circadian regulation. We detected prolonged expression of the core feedback loop components period and timeless, whilst the secondary feedback loop member vrille had lower expression rates in general. Our results show that the Drosophila HD model recapitulates most of the sleep related symptoms reported in patients therefore it can be a potential tool to study the molecular background of sleep defects in HD. Altered expression of circadian “clock” genes suggests that disturbed sleep pattern in HD might be the consequence of disturbed circadian regulation.
Raptor talon shape and biomechanical performance are controlled by relative prey size but not by allometry
Authors: Tsang Leah R. , Wilson Laura A. B. , Ledogar Justin , Wroe Stephen , Attard Marie , Sansalone Gabriele 
Published: May 2019

AbstractMost birds of prey (raptors), rely heavily on their talons for capturing prey. However, the relationship between talon shape and the ability to take prey is poorly understood. In this study we investigate whether raptor talons have evolved primarily in response to adaptive pressures exerted by different dietary demands, or if talon morphology is largely constrained by allometric or phylogenetic factors. We focus on the hallux talon and include 21 species in total varying greatly in body mass and feeding ecology, ranging from active predation on relatively large prey to obligate scavenging. To quantify the variation in talon shape and biomechanical performance within a phylogenetic framework, we combined three dimensional (3D) geometric morphometrics, finite element modelling and phylogenetic comparative methods. Our results indicate that relative prey size plays a key role in shaping the raptorial talon. Species that hunt larger prey are characterised by both distinct talon shape and mechanical performance when compared to species that predate smaller prey, even when accounting for phylogeny. In contrast to previous results of skull-based analysis, allometry had no significant effect. In conclusion, we found that raptor talon evolution has been strongly influenced by relative prey size, but not allometry and, that talon shape and mechanical performance are good indicators of feeding ecology.
The selective disruption of presynaptic JNK2/STX1a interaction reduces NMDA receptor-dependent glutamate release
Authors: Marcelli Serena , Iannuzzi Filomena , Ficulle Elena , Mango Dalila , Pieraccini Stefano , Pellegrino Sara , Corbo Massimo , Sironi Maurizio , Pittaluga Anna , Nisticò Robert , Feligioni Marco 
Published: May 2019

AbstractThe neuronal loss caused by excessive glutamate release, or ‘excitotoxicity’, leads to several pathological conditions, including cerebral ischemia, epilepsy, and neurodegenerative diseases. Over-stimulation of presynaptic N-methyl-D-aspartate (NMDA) receptors is known to trigger and support glutamate spillover, while postsynaptic NMDA receptors are responsible for the subsequent apoptotic cascade. Almost all molecules developed so far are unable to selectively block presynaptic or postsynaptic NMDA receptors, therefore a deeper knowledge about intracellular NMDA pathways is required to design more specific inhibitors. Our previous work showed that presynaptic c-Jun N-terminal kinase 2 (JNK2) specifically regulates NMDA-evoked glutamate release and here we demonstrate that an interaction between Syntaxin-1a and JNK2 is fundamental to this mechanism. Based on this evidence, a new cell permeable peptide (CPP), “JGRi1”, has been developed to disrupt the JNK2/STX1a interaction to indirectly, but specifically, inhibit presynaptic NMDA receptor signaling. JGRi1 reduces the NMDA-evoked release of glutamate both in in-vitro and ex-vivo experiments while also being able to widely diffuse throughout brain tissue via intraperitoneal administration. In conclusion, the JNK2/STX1 interaction is involved in presynaptic NMDA-evoked glutamate release and the novel CPP, JGRi1, acts as a pharmacological tool that promotes neuroprotection.
Mantle flow and deep electrical anisotropy in a main gateway: MT study in Tierra del Fuego
Authors: González-Castillo Lourdes , Bohoyo Fernando , Junge Andreas , Galindo-Zaldívar Jesús , Cembrowski Marcel , Torres-Carbonell Pablo , Ruiz-Constán Ana , Pedrera Antonio , Ibarra Pedro , Maestro Adolfo , Ruano Patricia 
Published: May 2019

AbstractAsthenospheric mantle flow drives lithospheric plate motion and constitutes a relevant feature of Earth gateways. It most likely influences the spatial pattern of seismic velocity and deep electrical anisotropies. The Drake Passage is a main gateway in the global pattern of mantle flow. The separation of the South American and Antarctic plates since the Oligocene produced this oceanic and mantle gateway connecting the Pacific and Atlantic oceans. Here we analyze the deep crustal and upper mantle electrical anisotropy of its northern margin using long period magnetotelluric data from Tierra del Fuego (Argentina). The influence of the surrounding oceans was taken into account to constrain the mantle electrical conductivity features. 3D electrical models were calculated to fit 18 sites responses in this area. The phase tensor pattern for the longest periods reveals the existence of a well-defined NW-SE electrical conductivity anisotropy in the upper mantle. This anisotropy would result from the mantle flow related to the 30 to 6 Ma West Scotia spreading, constricted by the subducted slab orientation of the Pacific plate, rather than the later eastward mantle flow across the Drake Passage. Deep electrical anisotropy proves to be a key tool for a better understanding of mantle flow.
VetTag: improving automated veterinary diagnosis coding via large-scale language modeling
Authors: Zhang Yuhui , Nie Allen , Zehnder Ashley , Page Rodney L. , Zou James 
Published: May 2019

AbstractUnlike human medical records, most of the veterinary records are free text without standard diagnosis coding. The lack of systematic coding is a major barrier to the growing interest in leveraging veterinary records for public health and translational research. Recent machine learning effort is limited to predicting 42 top-level diagnosis categories from veterinary notes. Here we develop a large-scale algorithm to automatically predict all 4577 standard veterinary diagnosis codes from free text. We train our algorithm on a curated dataset of over 100 K expert labeled veterinary notes and over one million unlabeled notes. Our algorithm is based on the adapted Transformer architecture and we demonstrate that large-scale language modeling on the unlabeled notes via pretraining and as an auxiliary objective during supervised learning greatly improves performance. We systematically evaluate the performance of the model and several baselines in challenging settings where algorithms trained on one hospital are evaluated in a different hospital with substantial domain shift. In addition, we show that hierarchical training can address severe data imbalances for fine-grained diagnosis with a few training cases, and we provide interpretation for what is learned by the deep network. Our algorithm addresses an important challenge in veterinary medicine, and our model and experiments add insights into the power of unsupervised learning for clinical natural language processing.
Caffeine-free hawk tea lowers cholesterol by reducing free cholesterol uptake and the production of very-low-density lipoprotein
Authors: Feng Juan , Yang Jian , Chang Yujun , Qiao Liansheng , Dang Honglei , Luo Kun , Guo Hongyan , An Yannan , Ma Chengmei , Shao Hong , Tian Jie , Yuan Yuan , Xie Lan , Xing Wanli , Cheng Jing 
Published: May 2019

AbstractMedicinal plants show important therapeutic value in chronic disease treatment. However, due to their diverse ingredients and complex biological effects, the molecular mechanisms of medicinal plants are yet to be explored. By means of several high-throughput platforms, here we show hawk tea extract (HTE) inhibits Niemann–Pick C1-like 1 (NPC1L1)-mediated free cholesterol uptake, thereby inducing the transcription of low-density lipoprotein receptor (LDLR) downstream of the sterol response element binding protein 2 (SREBP2) pathway. Meanwhile, HTE suppresses hepatocyte nuclear factor 4α (HNF4α)-mediated transcription of microsomal triglyceride transfer protein (MTP) and apolipoprotein B (APOB), thereby decreasing the production of very-low-density lipoprotein. The catechin EGCG ((−)-epigallocatechin gallate) and the flavonoids kaempferol and quercetin are identified as the bioactive components responsible for the effects on the NPC1L1-SREBP2-LDLR axis and HNF4α-MTP/APOB axis, respectively. Overall, hawk tea works as a previously unrecognized cholesterol-lowering agent in a multi-target and multi-component manner.