bluuebunny/crossref_metadata_2025_split huggingface dataset:
The variety of abomasal nematode communities of captive and free-roaming populations of European bison, Bison bonasus (L.): a morphometric and molecular approach
Authors: Gałązka Marta , Filip-Hutsch Katarzyna , Klich Daniel , Olech Wanda , Anusz Krzysztof , Pyziel Anna M. 
Published: November 2024

Abstract
Most studies concerning parasitic infections in European bison have been performed on free-ranging animals: comparatively little is known about the abomasal nematodes of captive wisents, which are widely used in reintroduction programmes. The aim of the study was to determine the infection level and species composition of abomasal nematodes in captive European bison in enclosures (including zoos) and breeding centres compared to free-ranging individuals. It also includes a morphological analysis of the parasites based on figures and measurement data. Altogether, 11 species of nematodes were detected, with both captive and free-ranging animals demonstrating similar species compositions. Among those, 2 species of blood-sucking nematodes were detected, including Ashworthius sidemi and Haemonchus contortus. Interestingly, A. sidemi was found in almost all free-roaming animals, but only in 1 captive European bison. In addition, H. contortus was predominant in captive animals. The morphological identification was confirmed molecularly for 5 nematode species: A. sidemi, H. contortus, Ostertagia kolchida, O. ostertagi and Spiculopteragia boehmi. The identification was performed using small subunit ribosomal rDNA. The study provides the first available set of specular lengths of the gastric nematodes of European bison, and the first molecular data of O. kolchida and S. boehmi derived from the same host species. Our findings may simplify the morphometrical and molecular identification of Trichostrongylidae species infecting European bison, and can be useful in developing new management strategies for populations of this near-threatened species in Europe.
New design and construction of a mechanical gripping device with a telescopic link of a fruit harvesting robot
Authors: Rybak Larisa , Carbone Giuseppe , Mohan Santhakumar , Gaponenko Elena , Malyshev Dmitry , Voloshkin Artem 
Published: November 2024

Abstract
Gripping devices for harvesting fruits have such types of work as cutting, tearing and unscrewing. For apples, it is preferable to use slicing or unscrewing, while the fruit leg should not remain, damaging the apple during storage. In this article, we are developing a grab for harvesting apples. The gripper is used both for holding the fruit and for jamming, followed by unscrewing. One of the advantages is that the proposed method of collecting apples allows you not to waste time moving the manipulator from the tree to the basket, but only to grab and tear them off. The fruit enters the gripper device; after which it enters the fruit collection container through a rigid or flexible pipe. The gripper device is built on the basis of a ball-screw transmission, which is supplemented by a gear drive along the helical surface. This allows for rotation and rectilinear movement of the held fruit. The gripping device has a ratchet mechanism that allows you to fix the fruit. A mathematical model of the gripper device has been developed, which allows determining the torque of the engine depending on the position of the fingers. The parameters of the mechanism were optimized using a genetic algorithm, and the results are presented in the form of a Pareto set. A 3D model of the gripper device has been built and a layout has been developed using 3D printing. Experimental laboratory and field tests of the gripping device were carried out.
Hopf bifurcations for a delayed discrete single population patch model in advective environments
Authors: Liu Weiwei , Shen Zuolin , Chen Shanshan 
Published: November 2024

Abstract
In this paper, we consider a delayed discrete single population patch model in advective environments. The individuals are subject to both random and directed movements, and there is a net loss of individuals at the downstream end due to the flow into a lake. Choosing time delay as a bifurcation parameter, we show the existence of Hopf bifurcations for the model. In homogeneous non-advective environments, it is well known that the first Hopf bifurcation value is independent of the dispersal rate. In contrast, for homogeneous advective environments, the first Hopf bifurcation value depends on the dispersal rate. Moreover, we show that the first Hopf bifurcation value in advective environments is larger than that in non-advective environments if the dispersal rate is large or small, which suggests that directed movements of the individuals inhibit the occurrence of Hopf bifurcations.
Recent kernel methods for interacting particle systems: first numerical results
Authors: Fiedler Christian , Herty Michael , Segala Chiara , Trimpe Sebastian 
Published: November 2024

Abstract
Interacting particle systems (IPSs) are a very important class of dynamical systems, arising in different domains like biology, physics, sociology and engineering. In many applications, these systems can be very large, making their simulation and control, as well as related numerical tasks, very challenging. Kernel methods, a powerful tool in machine learning, offer promising approaches for analyzing and managing IPS. This paper provides a comprehensive study of applying kernel methods to IPS, including the development of numerical schemes and the exploration of mean-field limits. We present novel applications and numerical experiments demonstrating the effectiveness of kernel methods for surrogate modelling and state-dependent feature learning in IPS. Our findings highlight the potential of these methods for advancing the study and control of large-scale IPS.
Associations between iron status and psychosocial wellbeing among pregnant women in Cape Coast, Ghana: a longitudinal study
Authors: Pobee Ruth A , Setorglo Jacob , Klevor Moses , Murray-Kolb Laura E. 
Published: November 2024

Abstract

Objective:
To determine the associations among iron status, depressive/anxiety symptoms, and quality of life (QoL) throughout pregnancy.

Design:
This longitudinal study recruited participants in their 1st trimester (< 13 weeks; n=116) and followed in their 2nd (n=71) and 3rd (n=71) trimesters. Sociodemographic, food security, anxiety, depressive symptoms, and QoL questions were collected. Hemoglobin (Hb), ferritin (Ft), and transferrin saturation (TSAT) were determined. Women were categorized as iron improvers or non-improvers based on changes in iron status. Associations were assessed using difference-in-difference analyses.

Setting:
Cape Coast, Ghana between October 2017 to September 2018.

Participants:
Pregnant women, 18-38 years.

Results:
Improvement in Ft levels from the 1st to 2nd trimester were associated with reduced depressive symptoms (-2.96 vs -0.58, p=0.028), and higher overall QoL (13.99 vs 1.92, p=0.006) particularly role physical (23.32 vs -2.55, p=0.025) and role emotional (27.50 vs 10.06, p=0.025) subscales. Improvement in Hb levels during the same period were linked to less anxiety, particularly fear factor (-2.62 vs -0.51, p=0.020); and worsened physical health aspect of QoL (-21.80 vs -3.75, p=0.005). Improvement in TSAT levels from 2nd to 3rd trimester were associated with increased total anxiety (1.56 vs -0.64, p=0.030) and panic factor (0.45 vs -0.26, p=0.004) and decreased total QoL (-1.08 vs 7.94, p=0.017), specifically role physical (-10.98 vs 11.93, p=0.018).

Conclusion:
Increases in iron status from first to second trimester were related to improvements in psychosocial wellbeing, implying potential benefit of iron supplementation on affect in early pregnancy. Larger studies are needed to confirm these findings.
Comparison of electronic and optoelectronic signal generation for (sub-)THz communications
Authors: Dittmer Joel , Tebart Jonas , Matalla Patrick , Wagner Sandrine , Tessmann Axel , Bhutani Akanksha , Koos Christian , Stöhr Andreas , Randel Sebastian 
Published: November 2024

Abstract
In recent years, the significance of terahertz (THz) and (sub-)THz communications has grown substantially due to its promising trade-off between higher capacity compared to microwave-based communication and better resilience against weather dependent influences (e.g., fog and rain). While electronic and optoelectronic techniques have been extensively explored, each offering distinct advantages and limitations, they have predominantly been demonstrated and discussed as individual experiments, making performance comparison challenging. This paper addresses this gap by systematically benchmarking electronic and optoelectronic signal generation approaches under comparable conditions. Our experiments incorporate various receiver types, revealing that best performance is achieved by combining optoelectronic signal generation techniques at the transmitter in combination with an all-electric intradyne receiver. This results in a remarkable line rate of 200 Gbit/s over a distance of 52 m. To our knowledge, this represents the highest line rate achieved for technically relevant transmission distances for indoor access or outdoor small cell networks.
Planar dual-band bandpass filter with regular harmonic suppression
Authors: Gupta Ajay , Khan Arani Ali 
Published: November 2024

Abstract
This paper presents a dual-band bandpass filter with regular harmonic suppression. The proposed third-order filter consists of two microstrip stepped impedance resonators and single substrate integrated waveguide resonator. The mismatches in their higher order resonant frequencies are utilized to suppress the regular harmonics. The passbands are centered at f1 = 2.4 GHz and f2 = 3.5 GHz with fractional bandwidths of 5% and 7.5%, respectively. The measured midband insertion and return losses are better than 2.55 and 14.5 dB for the first, whereas for the second band, they are better than 1.95 and 14 dB. The filter offers at least 33 dB suppression of first three higher order regular harmonics of f1 and f2.
SMAD4 Limits PARP1 dependent DNA Repair to Render Pancreatic Cancer Cells Sensitive to Radiotherapy
Authors: Wang Yang , Yu Tianyu , Zhao Zhangting , Li Xiaobing , Song Yiran , He Yazhi , Zhou Yingqun , Li Pu , An Liwei , Wang Feng 
Published: November 2024

AbstractDysregulation of SMAD4 (i.e. somatic mutation) is strongly associated with poor pancreatic ductal adenocarcinoma (PDAC) prognosis, yet the molecular mechanisms remain underlying this relationship obscure. Previously, we discovered that SMAD4 mutation renders pancreatic cancer resistant to radiotherapy via promotion of autophagy. In the current work, we observed a downregulation of the protein level of SMAD4 in PDAC as compared with adjacent normal tissue, and that such SMAD4low PDAC failed to benefit from chemotherapy. Furthermore, we observed that SMAD4 depletion dramatically enhanced DNA repair capacity in response to irradiation (IR) or a radiomimetic chemical. Interestingly, we found the radiomimetic chemical having induced a robust translocation of SMAD4 into the nucleus, where a direct interaction was shown to occur between the MH1 domain of SMAD4 and the DBD domain of PARP1. Functionally, the SMAD4-PARP1 interaction was found to perturb the recruitment of PARP1 to DNA damage sites. Accordingly, the combination of olaparib and radiotherapy was indicated in vivo and in vitro to specifically reduce the growth of SMAD4-deficient PDAC by attenuating PARP1 activity. Collectively, our results revealed a novel molecular mechanism for the involvement of the SMAD4-PARP1 interaction in DNA repair with a vital role in radiotherapy response in PDAC. Based on our set of findings, our findings offer a new combined therapeutic strategy for SMAD4 deficient PDAC that can significantly reduce pancreatic cancer radiotherapy resistance.
GNG5 is a novel regulator of Aβ42 production in Alzheimer’s disease
Authors: Li Chunyuan , Yang Yan , Luo Shiqi , Qiu Wenying , Wang Xia , Ge Wei 
Published: November 2024

AbstractThe therapeutic options for Alzheimer’s disease (AD) are limited, underscoring the critical need for finding an effective regulator of Aβ42 production. In this study, with 489 human postmortem brains, we revealed that homotrimer G protein subunit gamma 5 (GNG5) expression is upregulated in the hippocampal–entorhinal region of pathological AD compared with normal controls, and is positively correlated with Aβ pathology. In vivo and in vitro experiments confirm that increased GNG5 significantly promotes Aβ pathology and Aβ42 production. Mechanically, GNG5 regulates the cleavage preference of γ-secretase towards Aβ42 by directly interacting with the γ-secretase catalytic subunit presenilin 1 (PS1). Moreover, excessive GNG5 increases the protein levels and the activation of Rab5, leading to the increased number of early endosomes, the major cellular organelle for production of Aβ42. Furthermore, immunoprecipitation and immunofluorescence revealed co-interaction of Aβ42 with GPCR family CXCR2, which is known as the receptor for IL-8, thus facilitating the dissociation of G-proteins βγ from α subunits. Treatment of Aβ42 in neurons combined with structure prediction indicated Aβ42 oligomers as a new ligand of CXCR2, upregulating γ subunit GNG5 protein levels. The co-localizations of GNG5 and PS1, CXCR2 and Aβ42 were verified in eight human brain regions. Besides, GNG5 is significantly reduced in extracellular vesicles (EVs) derived from cerebral cortex or serum of AD patients compared with healthy cognition controls. In brief, GNG5 is a novel regulator of Aβ42 production, suggesting its clinical potential as a diagnosis biomarker and the therapeutic target for AD.
Irradiated whole cell Chlamydia vaccine confers significant protection in a murine genital tract challenge model
Authors: Broder Kieran C. , Matrosova Vera Y. , Tkavc Rok , Gaidamakova Elena K. , Ho Lam Thuy Vi Tran , Macintyre Andrew N. , Soc Anthony , Diallo Aissata , Darnell Stephen C. , Bash Sarah , Daly Michael J. , Jerse Ann E. , Liechti George W. 
Published: November 2024

AbstractChlamydia trachomatis infections are the most common bacterial STIs globally and can lead to serious morbidity if untreated. Development of a killed, whole-cell vaccine has been stymied by coincident epitope destruction during inactivation. Here, we present a prototype Chlamydia vaccine composed of elementary bodies (EBs) from the related mouse pathogen, Chlamydia muridarum (Cm). EBs inactivated by gamma rays (Ir-Cm) in the presence of the antioxidant Mn2+-Decapeptide (DEHGTAVMLK) Phosphate (MDP) are protected from epitope damage but not DNA damage. Cm EBs gamma-inactivated with MDP retain their structure and provide significant protection in a murine genital tract infection model. Mice vaccinated with Ir-Cm (+MDP) exhibited elevated levels of Cm-specific IgG and IgA antibodies, reduced bacterial burdens, accelerated clearance, and distinctive cytokine responses compared to unvaccinated controls and animals vaccinated with EBs irradiated without MDP. Preserving EB epitopes with MDP during gamma inactivation offers the potential for a polyvalent, whole-cell vaccine against C. trachomatis.
Analysis of abiotic and biotic stress-induced Ca2+ transients in the crop species Solanum tuberosum
Authors: van Dieren Annelotte , Schwarzenbacher Roland E. , Sonnewald Sophia , Bittner Andras , Vothknecht Ute C. 
Published: November 2024

AbstractSecondary messengers, such as calcium ions (Ca2+), are integral parts of a system that transduces environmental stimuli into appropriate cellular responses. Different abiotic and biotic stresses as well as developmental processes trigger temporal increases in cytosolic free Ca2+ levels by an influx from external and internal stores. Stimulus-specificity is obtained by a certain amplitude, duration, oscillation and localisation of the response. Most knowledge on stress-specific Ca2+ transient, called calcium signatures, has been gained in the model plant Arabidopsis thaliana, while reports about stress-related Ca2+ signalling in crop plants are comparatively scarce. In this study, we introduced the Ca2+ biosensor apoaequorin into potato (Solanum tuberosum, Lcv. Désirée). We observed dose-dependent calcium signatures in response to a series of stress stimuli, including H2O2, NaCl, mannitol and pathogen-associated molecular patterns (PAMPs) with stimuli-specific kinetics. Direct comparison with Arabidopsis revealed differences in the kinetics and amplitude of Ca2+ transients between both species, implying species-specific sensitivity for different stress conditions. The potato line generated in this work provides a useful tool for further investigations on stress-induced signalling pathways, which could contribute to the generation of novel, stress-tolerant potato varieties.
Bronze age supply chains between ancient Egypt and Nubia revealed by lead isotope analysis of kohl samples
Authors: Lemos Rennan , Tichindelean Matei , Kochergina Yulia V. Erban , Zaggia Caterina , Werkström Ludmila , Hocker Emma , Martinón-Torres Marcos 
Published: November 2024

AbstractWhile there is a considerable body of data regarding the sources of minerals employed in Bronze Age Egypt, the supply chains to Sudanese Lower Nubia are virtually unknown. This paper presents results of lead isotope analysis of 11 samples of kohl from C-group, Pan-grave and New Kingdom funerary contexts across the Debeira and Ashkeit areas in Sudanese Lower Nubia during the Bronze Age. The samples could be divided in two groups based on their lead isotope ratios. A comparison between the kohl samples from Sudanese Lower Nubia and galena ores from various mining sites on the Red Sea coast of Egypt indicated that some of the galena used in kohl mixtures in Sudanese Lower Nubia came from the Pharaonic mining site of Gebel el-Zeit. The second group of Nubian samples seems to have originated from an alternative galena source yet to be determined. This is the first time that kohl samples from Sudanese Lower Nubia are integrated into known northeast African networks of supply by using Lead isotope analysis.
Circadian desynchrony in early life leads to enduring autistic-like behavioral changes in adulthood
Authors: Mishra Abhishek , Lin Hao , Singla Rubal , Le Nam , Oraebosi Michael , Liu Dong , Cao Ruifeng 
Published: November 2024

AbstractCircadian rhythm regulates a variety of biological processes in almost all living organisms. Modern lifestyles, e.g. transmeridian travel, night shift, light at night, etc., frequently disrupt people’s regular sleep-wake cycles and create a misalignment (circadian desynchrony) between the natural environment and the endogenous body clock, and between different circadian oscillators within the body. The long-term consequences of circadian desynchrony on neurodevelopment and adult behavior remain elusive. Increasing clinical evidence supports a correlation between the disruption of the circadian system and neurodevelopmental disorders, such as autism spectrum disorders. Despite clinical correlations, experimental evidence is yet to establish a link between circadian disturbance in early life and adult behavioral changes. Here, using a “short day” (SD) mouse model, in which mice were exposed to an 8 h/8 h light/dark (LD) cycle mimicking a “shift work” schedule from gestation day 1 to postnatal day 21, we performed a battery of behavioral tests to assess changes in adult behaviors, including sociability, affective behaviors, stereotypy, cognition and locomotor functions. In contrast to the control mice kept in a 12 h/12 h LD cycle, the adult SD mice entrained to the 8 h/8 h LD cycle, but their free running rhythms remained normal in constant darkness. Interestingly, however, the SD mice displayed diminished sociability, a reduced preference for social novelty, excessive repetitive behaviors, and compromised cognitive functions, all of which resemble characteristics of autism-like behavioral alterations. In addition, the SD mice exhibited significant anxiety- and depressive-like behaviors and impaired motor functions. By western blotting and immunostaining analyses, hyperactivation of the mTORC1/S6K1 pathway was detected in multiple forebrain regions of SD mice. These findings underscore the enduring impact of early-life circadian disruption on neurochemical signaling and behavioral patterns into adulthood, highlighting a pivotal role for circadian regulation in neurodevelopment.
APOE from astrocytes restores Alzheimer’s Aβ-pathology and DAM-like responses in APOE deficient microglia
Authors: Preman Pranav , Moechars Daan , Fertan Emre , Wolfs Leen , Serneels Lutgarde , Shah Disha , Lamote Jochen , Poovathingal Suresh , Snellinx An , Mancuso Renzo , Balusu Sriram , Klenerman David , Arranz Amaia M , Fiers Mark , De Strooper Bart 
Published: November 2024

AbstractThe major genetic risk factor for Alzheimer’s disease (AD), APOE4, accelerates beta-amyloid (Aβ) plaque formation, but whether this is caused by APOE expressed in microglia or astrocytes is debated. We express here the human APOE isoforms in astrocytes in an Apoe-deficient AD mouse model. This is not only sufficient to restore the amyloid plaque pathology but also induces the characteristic transcriptional pathological responses in Apoe-deficient microglia surrounding the plaques. We find that both APOE4 and the protective APOE2 from astrocytes increase fibrillar plaque deposition, but differentially affect soluble Aβ aggregates. Microglia and astrocytes show specific alterations in function of APOE genotype expressed in astrocytes. Our experiments indicate a central role of the astrocytes in APOE mediated amyloid plaque pathology and in the induction of associated microglia responses.
Research on characteristic and DC voltage balancing control of a novel three‐phase line‐voltage cascaded unity power factor rectifier under unbalanced grid
Authors: Xia Liu , Cong Wang , Hong Cheng , Hua Chen , Jiayi Kong 
Published: November 2024

AbstractAccording to the operating modes of three‐phase line‐voltage cascaded boost high power factor rectifier topology, the operating characteristics of the rectified output voltage of each module and the characteristics of the topology for automatically balancing the input current under the unbalanced grid are analyzed in this article. Based on the analysis to the unique feature, a simple dual‐loop PI control strategy in the rotating coordinate system is designed with no need to deal with the negative sequence component of grid voltage. In response to the issue of unbalanced DC‐link capacitor voltage of each module caused by unbalanced grid, injection of the zero‐sequence voltage component is adopted in the designed control system, which make the output power of each module be able to be independently regulated, so that the balancing control to the DC‐link capacitor voltage of each module is realized. At the same time, the three‐phase input currents always remain in balance automatically operating under unity power factor. Simulations and experiments have verified the superiority of the topology structure and the feasibility of the control strategy.