bluuebunny/crossref_metadata_2025_split huggingface dataset:
LEVELS ANTIINFAMMATORY CYTOKINES (ІL-4, ІL-10) IN MAINTENCE HEMODIALYSIS PATIENTS DEPENDING ON EXPRESSED ANAEMIA AND CHARACTER DEFEAT OF KIDNEY
Authors: Dudar І. , Savchuk V. , Gonchar I. , Driyanska V. , Krasjuk E. , Khil M. 
Published: June 2018

Aims: The purpose of the present study was to compare the levels of anti-inflammatory cytokines in patients with chronic kidney disease, prolonged sessions of hemodialysis, depending on the severity of anemia.
Materials and methods: We investigated 155 patients with chronic kidney disease, prolonged sessions of hemodialysis and anemia (mean age 46.4±12.4). Patients were divided into 4 groups depending on expressed of anemia: in the I-st (n=22) group – patients with the level of hemoglobin over 110g/l, in the II (n=66) group hemoglobin 90 - 110g/l, in the III (n=44) group hemoglobin 70-89 g/l, in the IV (n=22) group hemoglobin <70 g/l.
Results: We have defined that level of IL-4 was clinically significantly lower (p <0.001) in patients, who have getting hemodialysis by comparison to control group. Levels of IL-4 had a significant differences between all of group of patients and controls (p <0.05). IL-10 had a positive correlation with the level of Hb (r=0.2405; p=0.003). ІL-4 had a negative correlation with the level of Hb (r=-0.4842; p <0.001).
The average level of IL-4 was significantly increased in diabetic patients compared with non-diabetic patients 92±17.7 vs 48.3±28.1 (p <0.05).
Conclusions: All of studied patients have had an increased level of anti- inflammatory cytokines. Serum IL-4 was higher in patients with diabetes. The levels of anti-inflammatory cytokines (IL-4 and IL-10) have had a significant correlation with the severity of anemia.
ASSOTIATIONS OF PECULIARITIES OF HLA-PHENOTYPE AND THE SENSIBILITY TO THE CORTICOSTEROID TREATMENT IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS WITH NEPHROTIC SYNDROME
Authors: Kolesnyk M. , Drannik G. , Driyanska V. , Petrina O. , Velychko M. 
Published: June 2018

The purpose of study was determination of HLA -antigens I and II classes as predictors of ineffectiveness of initial steroid therapy, and according prognozonegative markers of chronic glomerulonephritis with nephrotic syndrome.
Methods. In 59 chronic glomerulonephritis with nephrotic syndrome patients (steroid sensitive n=33 (1 gr.) and steroid resistant’s n= 26 (2 gr.)) and 350 healthy donors( control group) studied HLA antigens I and II classes of the special anti- HLA-antigens panel (20 antigens of locus A, 31 – of locus B and 9- of locus DR).
Result. In patients with chronic glomerulonephritis, nephrotic syndrome with hormone sensitivity relative risk is high at the presents of A28 (RR=8,5, r р <0,001), it made attributive risk (=0,37). In comparison with a control group, RR>2 for antigens A11 (RR=2,23), A23 (RR=4,28), A24 (RR=3,3), A29 (RR=10,78) that A30 (RR=11,23); attributive risk more than 0,1 for the antigen A11 (=0,16) ; A24
(=0,13), other did not differ from control. Subzero connection is exposed for the antigens of A2 (р<0,001), А9 (р=0,007). In locus antigen B14 (RR=5,65, р =0,001) are exposed, B44 (RR=48,25, р =0,004), B51(RR=12,32, р =0,006) and attributive risk of development of disease (according =0,24, 0,12 ; 0,14); and antigens B38 and B41 (RR=11,57, р=0,05). The steroid sensitivity was associated with the antigens B5 (p=0,033), B12 (p=0,005) and B35 (p=0,021). In locus DR made etiologic faction antigens DR4 (RR=7,0 and =0,24) DRw52 (RR=7,0 and =0,25).
Conclusions. For patients with chronic glomerulonephritis with a nephrotic syndrome antigens of HLA-B14,B38, B51, DRw52 are associated with steroid sensitivity. The attributive risk of steroid resistance is high for split A19+31+32, antigens B8, B55.
RELATIONSHIP OF LIPID PEROXIDATION PROCESSES AND CHRONIC INFLAMMATION WITH ATHEROSCLEROTIC VASCULAR CHANGES AND INDICATORS ENDOTHELIAL DYSFUNCTION IN PATIENTS WITH CKD STAGES II-IV
Authors: Loboda O.M. , Krasyuk І.V. , Alexeeva V.V. , Korol L.V. , Druanska V.Y. , Dudar I.O. 
Published: June 2018

The aim of our study was to determine the relationship of processes of atherogenesis and indicators of endothelial dysfunction with the processes of activation of lipid peroxidation (LPO) and chronic inflammation in patients with CKD stages II-IV.
Material and methods. Levels of proinflammatory and anti-inflammatory cytokines, indicators of lipid peroxidation, the thickness of the intima-media (IMT) of the carotid artery, ankle-brachial index (ABI) were measured in 90 patients with CKD stage II-IV (30 - CKD st. II, 31 patients with CKD st. III and 29 st. IV CKD patients) and 30 healthy subjects (control group). The correlation analysis was performed to identify the possible association between the obtained parameters of atherosclerotic vascular lesions and endothelial dysfunction and indices of lipid peroxidation and pro- and anti-inflammatory cytokines.
Results. The average levels of interferon-y, interleukin (IL) -1p and IL-10 in patients with CKD II-IV Art. were significantly increased compared with the corresponding values in the control group. The average levels of malondialde- hyde (MDA) of serum and MDA of erythrocyte in CKD patients was significantly increased compared with the corresponding values in the control group, and the antioxidant system indices - total peroxidase activity of erythrocytes and serum levels of the sulfhydrylgroups was significantly reduced relative to the corresponding values in the group control. 39 (43%) patients with CKD st. II-IV were recorded carotid atherosclerotic changes. The IMT (from 0.91 to 1.29 mm) defined in 22 (56%) patients and atherosclerotic plaques (IMT > 1,3 mm) defined in 17 (44%) patients. Bilateral carotid atherosclerotic lesions was observed in 14 (36%) patients with CKD st. II-IV. Endothelial dysfunction was observed in 71 (79%) patients with CKD st. II-IV. There was a statistically significant association between indicators of chronic inflammation, lipid peroxidation and indicators of atherosclerotic vascular lesions and endothelial dysfunction.
Conclusion. Pathological processes ofLPO activation, chronic inflammation are closely linked between themselves and progression of atherosclerotic vascular lesions in CKD.
THE ETIOLOGICAL SPECTRUM AND ANTIBIOTIC RESISTANCE PATTERN OF BACTERIA CAUSING UNCOMPLICATED URINARY TRACT INFECTIONS: A TEN-YEAR SURVEILLANCE STUDY (2005-2015)
Authors: Kolesnyk M. , Stepanova N. , Kruglikov V. , Rudenko A. 
Published: June 2018

Background and objective. Knowledge of local antimicrobial resistance pattern is very important for evidence-based empirical antibiotic prescribing. The main objective of the present study was to evaluate the prevalence and the antimicrobial resistance pattern of the main bacteria responsible for uncomplicated urinary tract infection (UTI) in Kyiv region (Ukraine), throughout a ten year period, in order to establish an appropriate empirical therapy.
Materials and methods. A retrospective analysis of the etiological spectrum and antimicrobial resistance of uropathogens in urine samples isolated over the 10-year period, 2005 to 2015, in a single center was performed.
Results. In total 380positive urine samples processed at our laboratory of which 193/380 (51 %) had E. coli as the infecting organism. Although E. coli was, as usual, the most common pathogen implicated in UTI, it were observed increasing the share of Enterococcus spp. - 82/380 (21.6%). Ampicillin and trimethoprim were the least-active agents against E. coli with resistance rates of 75% and 70%, respectively. Significant trends of increasing resistance over the 10-year period were identified for trimethoprim, fluoroquinolones ІІ and III generations, penicillins, and carbapenems. Nitrofuran derivative remains a reasonable empirical antibiotic choice in this community with a 10-year resistance rate of 8.3 %. Was
determined that recurrent UTI is an independent risk factor for bacterial multidrug-resistance.
Conclusions. Over the last 10 years, the proportions of fluoroquinolones resistant E. coli and multidrug-resistant bacteria have significantly increased. The fluoroquinolones shall not be used in the empirical treatment of uncomplicated
UTI in Kyiv region patients. For the empirical treatment of uncomplicated UTI in women should be used nitrofuran derivative (furazydyn K). If required of parenteral administration of antibiotics should be used cephalosporins IVgeneration. This data will enable evidence-based empirical prescribing which will ensure more effective treatment and lessen the emergence of resistant uropathogens in the community.
EFFECT OF COMBINATION THERAPY WITHFOSINOPRIL AND MELDONIUM ON THE INDICES OF 48-HOUR MONITORING OF BLOOD PRESSURE AND MYOCARDIUM GEOMETRY IN HEMODIALYSIS PATIENTS WITH CHRONIC HEART FAILURE
Authors: Salyzhyn T.I. 
Published: June 2018

Despite medical advances, cardiovascular complications continue to dominate in patients treated with hemodialysis. Therefore, the search for new methods of diagnosis and treatment is advisable.
The objective of our research was to study the effectiveness of combination therapy with Fosinopril and Meldonium in patients with stage У chronic kidney disease (CKD) with the signs of chronic heart failure.
Materials and methods of the research: 60 patients with stage У CKD were examined and treated. They were on out-patient treatment with program hemodialysis at the Department of extracorporal methods ofdetoxification and chronic dialysis.
Results. Significant decrease in left ventricular mass (LVM) by 17.20% (p<0.001) and LVM index by 13.40% (p<0.001) was detected in patients treated with combination of Fosinopril and Meldonium Dehydrate in addition to background therapy. Regression of both concentric and eccentric left ventricular hypertrophy equally occurred under the influence of combination therapy constituting 53.6% vs. 40.2% and 33.4% vs. 26.6%, respectively. The variability of systolic blood pressure (SBP) was found to decrease significantly by 36.02% (p<0.001). The survival analysis provided an opportunity to determine a reliable difference between the group treated with background therapy and the addition ofa combination of two drugs.
Conclusions. The addition of Fosinopril and Mel- donium to the background dialysis therapy significantly improved the structural and geometric cardiac indices, positively affected the variability of SBP, increased the prognosis of cumulative survival compared to the background therapy.
PYLONEPHRITIS IN GIRLS: FEATURES AT THE MODERN STAGE
Authors: Vakulenko L. I. 
Published: June 2018

Girls performed the vast majority of patients with urinary system infections. The prevalence of acute disease and specific gravity of chronic kidney impartments have been increasing with the age and were often accompanied with concomitant urological, gynecological and sexual pathologies.
The purpose of the work was to determine the etiological and clinical preconditions of relapse and chronic pyelonephritis in girls.
Materials and methods. An analysis of the clinical course of187 cases ofpyelonephritis in girls of the age of1 - 18 years had been performing in period from 2008 to 2018 years. The 60 patients in observed group had acute process and 127 ones were with the chronic course of the disease.
Rresults. E. coli was the main etiological factor of the inflammatory process in kidneys. Thus, cases of acute and chronic pyelonephritis composed 63,5% and 44,2% in observed group appropriately. Furthermore, the proportion of grampositive flora increased in the cases of chronic pyelonephritis. In addition, almost 56,7% ofall girls with pyelonephritis had sexually transmitted infections, more likely the patients with chronic forms of the disease (69,3% and 30,0%, respectively (p <0,01). There was domination of the Chlamydia infection in the structure of these pathogens. The research revealed Chlamydia pathogen in the majority of cases — in 29,2% of the examined for urogenital infections girls with pyelonephritis. The presence of mycoplasma infection - 22.1%, trichomonas - 11.5%, candidiasis - 19.5% was less common. 24.8% of girls had clinical signs of vulvitis without identifying the etiological factor
Conclusions. E. coli prevails among the etiological factors of pyelonephritis in girls. Most of the girls with pyelonephritis with the recurrent and chronic course have urogenital infections. Moreover, association of bacterial microflora and sexually transmitted infections complicates the course of chronic pyelonephritis in girls; promotes the development and maintenance of urodynamic disorders, frequent relapses and progression of chronic inflammatory process in kidneys. In conclusion, urogenital infection examination should be mandatory for the girls with pyelonephritis.
THE CONTENTS OF SCD40 AND INDICATORS OF DAMAGED ENDOTHELIUM IN PATIENTS WITH CHRONIC KIDNEY DISEASE
Authors: Topchii I. I. , Kirienko А. N. , Dunaevskaya M. M. , Schenyavskaya Е. N. , Birukova I. T. 
Published: June 2018

Introduction. Impairments of endothelial structure and functions of renal vessels are regarded as important factors of progression and chronization of chronic kidney disease (CKD). It is well known that the CD40/CD40L interaction is responsible for the aggregation of blood cells and their adhesion to the vascular wall, as well as its effect on the state of immune response. We investigated whether this interaction could be altered in patients with CKD and impaired immune status.
Materials and methods. We examined 48 patients with chronic glomerulonephritis (CGN), 38 patients with chronic pyelonephritis and 42 patients with essential hypertension (EH) - control group. All patients received standard therapy with an ACE inhibitor perindopril in combination with a calcium channel
antagonist amlodipine. Along with standard therapy the patients intravenously administered injections of "Kardioarginine." The degree of endothelial damage was assessed by the number of CEC and VE-cadherin content in blood plasma. In order to estimate changes in the immune status we determined the content of sCD40 in serum.
Results. Increase in the content of serum sCD40 was shown for the CGN patients compared with healthy subjects and EH patients. The level of sCD40 in serum correlated with the number of CEC and VE-cadherin content in plasma. The use of kardioarginine combined with standard therapy led to reduction of sCD40 and stabilized the structure of endothelium. The loss of endothelial integrity in patients with CKD is apparently due to the activation of apoptosis with subsequent accelerated desquamation under the influence of activated white blood cells and the system of cytokines.
Conclusions. The increase of sCD40 in patients with CKD may be a compensatory mechanism aimed to reduce the inflammatory activity by blocking the CD40/CD40L interaction at the cellular level.
HEART RATE VARIABILITY IN HEMODIALYSIS PATIENTS
Authors: Loboda O. M. , Dudar I. O. , Gonchar Y. I. 
Published: June 2018

There are a small number of works that have studied heart rhythm variability parameters in patients treated with hemodialysis.
Aim. To investigate the peculiarities of heart rhythm variability parameters in patients treated with hemodialysis.
Methods. The observational controlled study included 55 patients treated with hemodialysis, aged 24 to 75 years, including 29 men and 26 women. The control group consisted of 20 healthy individuals of comparable age and sex. Heart rhythm variability parameters were investigated in all patients.
Results. In the study of heart rhythm variability in patients treated with hemodialysis comparatively with the control group time parameters and most of frequency indexes were decreased.
Conclusions. Our study found the diminishment of the total power of neurohumoral regulation in patients treated hemodialysis, as evidenced by the low values SDNN, rNN50, CV.
THE CORRELATION OF MARKERS OF ENDOGENIC INTOXICATION AND IMMUNITY IN PATIENTS WITH CHRONIC PYELONEPHRITIS AND EFFICACY OF DIFFERENT SCHEMES OF TREATMENT
Authors: Mylnikova T. , Martynuk L. , Symko G. , Yakubyshyna I. 
Published: June 2018

The purpose of study was to analize the correlation between the rate of syndrom of endogenic intoxication (EI) and the markers of immunity in patients with chronic pyelonephritis (PN). To research the effectiveness of different schemes of treatment chronic pyelonephritis.
Methods. Researched were the syndrom of EI and the some markers of immunity in 135 patients with chronic PN. The correlation analysis was performed. For estimate of effectiveness of different schemes of treatment the patients were divided into 4 groups.
Results. A growing of deficit of T-cells immunity markers such as CD3+, CD4+ та CD8+ was found and the rising of rate Ig А, G and the circulated immune complexes with increasing of EI. The research showed the rising of spontaneous production TNF- ά and decrease of rate IL-10 with deepening of intoxication.
Conclusions. Decreasing of cell-mediated and increasing of humoral immunity markers in patients with exacerbation of chronic PN were found. It was found that there was faster regress of clinical symptomatic in patients that got prescriptions treatment including Kanephron and Chophytol.
MICROBIOCENOSIS IN CHILDREN WHITH CHRONIC RECURRENT PYELONEPHRITIS.
Authors: Lavrenchuk О. 
Published: June 2018

Introduction — the leading mechanism of the kidney infections is the lymphogenous pathogen migration associated with translocation from the mesenteric lymph nodes and blood stream. Reduction in the number of bifidobacteria in the bifidobacteria\enterobacteria balance is an adverse prognostic factor of probably bacteria persistence in the urinary system.
The aim of our study was to compare the urine microflora with the microbiocenosis of the intestinal mucous membrane as well as of the nose and throat mucosa in children with recurrent pyelonephritis (PN).
Material and methods. Qualitative and quantitative investigation of the microbiological spectrum was per- fomed. In patients with the revealed e. coli the intestinal biocenosis was compared with the biocenosis of the nose and throat mucousa. The repeteated examination of the urine and feces was conducted in 12patients after the probiotic treatment.
Results. The predominance of E. coli and conditionally pathogenic microorganismus in the urine tests for patients with chronic PN in active stage as well as in remission coincided with the indicators of the intestine biocenosis. Consistently high inoculation of S. aureus in the nasopharynx of children with acute and chronic PN correlated with the presence of this agent in the urine and feces. Prolonged use of probiotics led to decrease ofenterokoccus and eliminated pathogenic enteroflora, as well as to PN remission with lack of intercurrent respiratory infection in the
dynamics of observation.
Conclusion.Comparison of urine microflora with nasopharigeal mucosa as well as with intestinal mucosa microflora in patients with recurrent chronic PN gives the opportunity to predict the clinical bechaviour and to correct individually the treatment using probiotics whith antirecurrent purpose.
SPECTRUM OF MICROFLORA IN URINE OF PATIENTS WITH CHRONIC RECURRENT PYELONEPHRITIS (THE TEN YEARS FOLLOWING-UP)
Authors: Rudenko A. , Kruglikov V. , Stepanova N. , Lebid L. , Kolesnyk M. 
Published: June 2018

The aim of this work was to compare the specific spectrum of bacterial pathogens which stipulate chronic recurrent pyelonephritis during the longitudinal following-up.
Materials and methods. There were performed the complex microbiologic investigations of urine, scrapes from cervical canal and vaginal smears from 386 women with chronic pyelonephritis.
Results. The analysis of the spectrum of microflora separated out of the patients’ urine during 10 years showed the considerable growth of the specific weight of Enterococcus spp. (up to 18,6 %). In the process of chronic pyelonephritis recurrence in 64,3 % patients showed the changes in the specific spectrum ofpathogens with the prevalence of E.faecalis and S.epidermidis (14,3 % each). At the same time, there was stated the presence of opportunistic microflora in vaginal smears (40,1 %) and scrapes from cervical canal (30,8 %) of the examined women. The mechanism of bacteria persistence in urogenital tracts in women with chronic recurrent pyelonephritis was also under study.
Conclusion. There has been confirmed the importance of the comprehensive study of the spectrum and specificity of pathogens which can stipulate the aggravation of chronic pyelonephritis and mechanisms of its course.
EFFICACY OF SODIUM NUCLEINATE INCOMPLEX TREATMENT OFPATIENTS WITH RECURRENT PYELONEPHRITIS
Authors: Stepanova N. , Driyanska V. , Kruglikov V. , Lebid L. , Kornilina O. , Romanenko O. , Kolesnyk M. 
Published: June 2018

The aim of our study was to determine the effectiveness of Sodium nucleinate in treatment of patients with recurrent pyelonephritis.
Material and methods. This clinical study is a prospective, open, controlled, randomized clinical study. Two types of therapies were evaluated in parallel groups. 98patients with recurrent pyelonephritis were examined in the dynamics of treatment. We investigated the state of the genitourinary system microbiota, mucosal immunity performance of the urogenital tract and contents of blood cytokines. After the examination the patients were divided by the applied therapeutic approach. The first group of the women (n=55) received antibiotic therapy and Sodium nucleinate 0.25 g 4 times per day for 2 weeks. The second group of the women (n=43) received only antibiotics.
Results. The frequency of eradication of U. urealyticum (including in association with M. hominis) was significantly higher in patients of the first group. We have identified a significantly higher phagocytic activity and a phagocytosis intensity of neutrophils in samples from the vagina in women of the first group (46 [28-49] vs 17 [13-27.5] p = 0.002 and 3.19 [2.34-4.9] against 2.8 [1.23-3.6] p=0.02, respectively).The study of mucosal immunity has demonstrated a significant decrease the content of lactoferrin and increasing sIg A in the urine of patients of group (6.85 [0.2-80] vs 0 [0-0.4] ng/ml, p=0.01 and 0.42 [0-0,7] vs. 3.2 [0-3,7]g/ml, p=0.04, respectively).In addition, the use of Sodium nucleinate promoted to a significant reduction of high levels of all investigated cytokines: IL-4 (p=0.003), IL-17 (p=0.005), NGAL (p=0.02), TNF-$ (p=0.02) and MCP-1 (p=0.03). In the control group was achieved just the reduction in levels of IL-4 (p=0.007) and IL-17 (p=0.04).
Conclusions. Thus, the application of the proposed therapies can improve the effectiveness of the treatment of patients with recurrent pyelonephritis by normalization of mucosal immunity of the genitourinary system and the cytokine profile. We believe that the traditional antibiotic therapy ofpatients with recurrent pyelonephritis advisable to supplement of Sodium nucleinate at a dose of 0.25g 4 times / day for 2 weeks.