bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antagonistic Endophytic Fungi of Hedychium coronarium J. Koenig from Hutan Sibayak and Taman Hutan Raya, North Sumatra against Staphylococcus aureus ATCC® 29213TM
Authors: Lutfia A , Munir E , Yurnaliza  
Published: July 2019

Abstract
Possible role of endophytic microbes has been receiving much attention from different aspects and all plants are believed to have an endophytic association. Some medicinal plants have been reported to harbor endophytic microbes capable of producing bioactive compounds. Endophytes may benefit plant host by secreting similar bioactive compounds leading to systemic plant defenses through synthesis of antimicrobial compounds. In this study, we reported that rhizome of Hedychium coronarium J. Koenig (a member of Zingiberaceae), native of Hutan Sibayak and Taman Hutan Raya, North Sumatra, is inhabited by various endophytic fungi morphotypes. Total of 28 fungal morphotypes were recovered from rhizome parts and differentiated based on morphological characters. Most fungal strains displayed antibacterial activities ranging from 0,00 (no activity) to 30 mm (very strong activity) against Staphylococcus aureus ATCC® 29213™. Based on this preliminary result, we may propose some antagonistic strains as candidates for further study regarding to the production of antibacterial compounds.
Effectiveness of chitosan from crab shell as antibiotic for Escherichia coli
Authors: Boy E , Ummah Z A 
Published: July 2019

Abstract
Background: Research in finding a safe, effective and natural ingredient against Escherichia coli continues to be carried out as a result of an increasing antibiotic resistance. Chitosan has been known as a food preservative but the research for its effectivity against Eschericia coli is still inadequate. Indonesia is rich of natural animal resources including crustaceans, like Crab. Objective : How effective are chitosans extracted from crab shells as an antibiotic against Escherichia coli when compared with ceftriaxone. Method : Experimental method with a static group comparison where E. coli in Mueller Hinton Agar (MHA) on petri dishes was given chitosan intervention with initiating concentrations starting from 4% to 8% and 30mcg of ceftriaxone given by diffusion. The antimicrobial of E.coli was measured with Whatman paper and standard calipers measured in millimeters. Result : Results of normality and homogeneity variance tests with (p > 0,05) were inhomogenous and not distributed normally so it was analyzed with Kruskal Wallis Test continued with a Mann Whitney Test. Conclusion : Chitosan solution extracted from crab shells with concentrations of 4%, 5%, and 6% have an antibiotic effect against the growth of E.coli. The most effective concentration to inhibit E.coli is 4%.
Molecular subtyping and dna sequencing homology of Escherichia coli O157:H7 isolated from Aceh cattle
Authors: Abrar M , Ferasyi T R , Amiruddin  , Fakhrurrazi  , Erina  , Razali  , Sabri M , Abdullah H , Zainuddin  , Haris A , Safika  , Dewi M , Barus R A 
Published: July 2019

Abstract

Escherichia coli (E. coli) O157:H7 is one of bacteria that recognized as pathogenic agent, both in animals and human. Several isolates of this bacteria were obtained from local livestock, such as in bali cattle and aceh cattle. However, the bacteria found in aceh cattle has not been confirmed through molecular technique. The current study was aimed to confirm the finding of E. coli strain O157:H7 in aceh cattle using molecular analysis. Three isolates of E. coli O157 (MA-35, MA-44, and MA-58) obtained from aceh cattle were extracted for the DNA using Presto™ Mini gDNA bacteria kit (Geneaid) through several processing stages of sample preparation, lysis, DNA binding, washing and elution. Then, it was continued with DNA Amplification Using Polimerase Chain Reaction (PCR). DNA sequencing homology was conducted using BLASTN program. Data of this study were analysed descriptively. The results showed that one of isolate (MA-58) was detected for the presence of genes of rfb (339 bp), fliC (401 bp), and stx2 (115 bp). Then, this isolate has a homology of 100% to 16 different strains of E. coli O157:H7 sequence in the GenBank. In conclusion, the molecular analysis of the local isolate of bacteria of MA-58 obtained from aceh cattle confirmed that it is E. coli O157:H7 and detected with the presence of genes of rfb, fliC, and stx2.
Distribution of Nitrate, Phosphate, Dissolved Oxygen and Macrozoobenthos Density in Belawan River
Authors: Yeanny Mayang Sari , Barus Ternala Alexander 
Published: July 2019

Abstract
Belawan River has many community activities such as residential areas, industries, Steam-electric power station, PDAM (local water company) and aquaculture. With this activity the waste is directly discharged into the river water, causing negative impacts on its aquatic environment such as nutrients (Nitrate and Phosphate) and water quality (Dissolved Oxygen) and the state of aquatic biota (Macrozoobenthos). One approach is to use the Purposive Random Sampling method with 5 stations. The approach was in the form of Macrozoobenthos measurement, namely; measurement of density, relative density, and attendance frequency. Measurement of nutrients, namely nitrate, phosphate and measurement of water quality, namely dissolved oxygen. The research results showed that Macrozoobenthos obtained as many as 9 genera. The highest Macrozoobenthos density in the genera of Polymesodaerosa was 629.62 ind/m2 at station 4, and the lowest was in the genera of Melanoides sp (Station 1), Macrobrachium sp and Melanoides sp (Station 2), Hirudo (Station 4), and Scylla sp (Station 5) with a density of 3.703 ind/m2. Based on nutrient nitrate concentration of 1.18-7.57 mg/l, it is included as mesotrophic and eutrophic waters, based on phosphate concentrations of 0.14-0.18 mg/l, it is included as hypertrophic, and dissolved oxygen of 3.8-5.3 mg/l is included as good water oxygen conditions.
Microsatellite analysis on oil palm (Elaeis guineensis) tolerance to Ganoderma boninense
Authors: Basyuni M , Afandi D , Hayati R , Bimantara Y , Arifiyanto D , Syahputra I 
Published: July 2019

Abstract
Palm oil plants (Elaeis guineensis) are nowadays an economically significant crop. Oil palm plantations in Asia, especially in Indonesia and Malaysia encounter the threat of basal stem root (SBR) diseases caused by Ganoderma boninense. The research aimed to analyze the microsatellite markers of oil palm tolerance to G. boninense. The plant material used in this observation was two cross-series of genetic material belonging to PT Socfin Indonesia. The materials have been known as the level of resistance to Ganoderma, the first is cross-series of 15-year-old oil palm in the field, and the second is a new cross-series for early detection in the nursery stage. The quality and quantity test showed that DNA was successfully isolated and can then be used for DNA amplification. The seven primers with DNA banding patterns clearly detected visible in one band. The target genes associated with resistance to SBR disease are found in almost all individual oil palm plants. Furthermore, the low PIC (Polymorphic Informative Content) value of the seven primers except for the Rgen_Pto primer showing the high PIC in the leaf tissue and mature plant roots, suggested the potential molecular marker of this primer for selection of tolerant palm against G. boninense.
Clarification of Elaeis guineensis from NCBI databases
Authors: Hayati R , Basyuni M 
Published: July 2019

AbstractOil palm is the essential oil-producing of the plant in the world. Two species genus in oil palm wereElaeis oleíferaandElaeis guineensisJacq. The National Center of Biology Information (NCBI) contain databases of biology, related information aboutE. Guineensis, E. Oleiferaand many other plant species. Identification of genes, DNA, proteins, active compounds and important taxonomies updated central server for researchers in the world. There are five numbers that have been a file in the source literature, included Bookshelf, Mesh, NLM Catalog, PubMed and PubMed Central literature. It found four books and reports and one Medical Subject Headings (MeSH), one NLM catalog reported, 346 PubMed and 1035 full-text journal articles aboutE. guineensishave been published. Furthermore, three assemblies, 43 BioProject, and 507 BioSample also available. Only exist one genome sequencing available in oil palm with 250.064 total DNA and RNA sequenceE.guineensis.The NCBI collected 41.353 EST and 30.746 genes. There is also 51.353 groups of protein, and 30.746 sequences of protein have been reported.
The bioinformatics method of protein dehydration induced drought stress in Hevea brasiliensis Muell. Arg
Authors: Pasaribu SA , Basyuni M , Hayati R 
Published: July 2019

Abstract

Hevea brasiliensis Muell. Arg. (rubber plant) is one of the most significant sources natural rubber in the world. Planting material used is vegetative propagation that called a clone. Each rubber clone has specific adaptability. Adaptation of rubber in drought area is a complex trait. The current study assesses of the bioinformatics on the NCBI database as well as expected the physicochemical of protein dehydration. Several parameters of physicochemical protein dehydration in H. brasiliensis were varied among the genes observed. There is twenty (20) protein dehydration induced from H. brasiliensis deposited in NCBI. The length of the genes was alternated with the genes ascertained. Several lines of coded amino acids were 370 to 1490 bp. The mitochondria target peptide value diversified from 0.020 to 0.342, signifying that it is expected to be a presence. The present resulted indicated the prominence of the variation and role of a physical and chemical characteristic of the distinct amino acids in protein dehydration genes as drought stress in rubber.
The effect of Plesiochrysa ramburi (Schneider) (Neuroptera: Chrysopidae) to the resilience of papaya invested by Paracoccus marginatus
Authors: Pramayudi Nur , Bakti Darma , Tobing Maryani Cyccu , Husni  
Published: July 2019

Abstract

Paracoccus marginatus is one of the important pestson papaya. This study was conducted to evaluate the presence of one of the Chrysopa predators, Plesiochrysa ramburi to papaya invested by P. marginatus. The study used the non-factorial design with the factor of the number of predators (1, 3, 5, 7), which was released to oneovisac of P. marginatus. All treatments in the experiment had five replications. Descriptively, this study showed that the presence of Pl. ramburi, positively, affected the resilience of the plant, in this case through the mechanism of population control of P. marginatus. The presence of predators, negatively, affected P. marginatus eating behavior, which was reduced food intake behavior, which was influenced by feeding behavior. Thus it could increase plant resilience. If the presence of predators negatively affected pest eating behavior, the presence of predators could positively influence plant resilience.
The Application of Municipal Waste Compost to Improve the Physical Properties of Soil and Palm Oil Production in Silinda District, Serdang Bedagai
Authors: Simaremare Parlindungan , Rauf Abdul , Hanum Hamidah 
Published: July 2019

Abstract
This research had been done in Agricultural faculty of north Sumatera university, medan on april until oktober 2009. The aim of this research are studied The Application of Municipal Waste Compost to Improve the Physical Properties of Soil and Palm Oil Production in Silinda District, Serdang Bedagai. This research was designed by 2 factors and 4 replications. The first factor are uses a separate plot design (RPT) with 2 factors and 4 replications as follows: Main plot consists of 2 levels of treatment, namely:T1: 5 years of planting, T2: 20 years of planting. Child Plots consist of 3 levels of treatment, namely: K1: Without Conservation Measures + Without Giving Compost, K2: Concave Plate + Compost, K3:, Individual Rorak + Compost, and numbers of reports: 4 replications (each planting). This result showed that the application of Municipal Waste Compost not significant to the Physical Properties such as,bulkdensity,porosity, permeability.
Physical Quality of Longissimus Muscles of Kacang Goat After Supplemented With a Combination of Palm Kernel Meal and Powdered Katuk Leaf
Authors: Razali  , Ferasyi Teuku Reza , Azhari  , Rastina  , Sabri Mustafa , Abrar Mahdi , Amiruddin  , Anwar  , Hutasoit Rijanto , Tarigan Andi 
Published: July 2019

Abstract
The purpose of this research was to analyzed the effect of supplementation with palm kernel meal (BIS), katuk leaf powder (KAT), and both of those combined to the physical quality of Longissimus dorsi (L. dorsi) muscle in Kacang goat. Twenty male Kacang goat were used in this research; which were aged of ±1.5 years and weight of 15,42 ± 1.28 kg live body weight (BW). Complete randomized design was used in this research; with 4 treatments and 5 replications. All goats were acclimatized for 2 weeks. Group P0 as control was given distilled water. Group P1 was given BIS at dose of 100 gr/day/head. Then, Group P2 was administered BIS at dose of 100gr/day/head combined with powdered katuk leaf at dose of 15 gr/day/head, and Group P3 was given KAT 15gr/day/head, each goat was received treatments twice per day (at morning and afternoon) for 35 consecutive days. All animals were fed with leaves and grasses, and had access to water ad libitum. At the 25th and 50th day after treatment, goats from each group were slaughtered according to standard commercial halal guidelines. Meat samples derived from L. dorsi muscles were taken and to be tested for physical quality of pH, shear force, water holding capacity, cooking loss, meat and fat color. Data were analyzed by using analysis of variance. The administration of KAT tended to lower (P > 0,05) the shear force value of the L. dorsi muscle as compared to control group. In conclusion, the administration of powdered katuk leaves is appropriate to improve the physical quality of L. dorsi muscles in local Kacang Goat.
Antibacterial Ability of Endophytic Bacteria Isolated from Kemenyan (Styrax benzoin L.)
Authors: Siregar R V , Suryanto D , Yurnaliza  
Published: July 2019

Abstract

Kemenyan is one of plant commodities in North Sumatera that has been used as medicinal plants to treat infection and illness. The aim of this study was to isolate endophytic bacteria from kemenyan (Styrax benzoin) and evaluate their antibacterial properties against pathogenic bacteria. Sixteen isolates were found from leaf, bark and root samples of kemenyan. Two isolates, RS01 and RS13 showed considerable antibacterial activities against Staphyloccocus aureus, Escherichia coli and Streptococcus mutans.
Decolorization of Azo Synthetic Textile Dye by Halotolerant Bacteria And Identification Used By 16S rRNA
Authors: Hutagalung D. E , Hannum S , Priyani N 
Published: July 2019

Abstract
Textile dye has been used in textile industry to produce carpet, batik, cosmetics as well as pharmaceutical products. Utilizing of textile dye especially azo dye in textile industries is more efficient than natural dye. The high production of textile increases liquid wastes. If the liquid waste is not treated properly and discharged directly to environment, it causes pollution that could harm both organism and the environment itself. Azo textile dye is difficult to be degraded in the naturally environment. The aim of this research is to study the potency of halotolerant bacteria to decolorize azo textile dye. The isolates were isolated from the sea around of Mursala Island, Tapian Nauli village, Central Tapanuli district. In this research, the highest percentage of decolorization was shown by 2 isolates, DH2 and DH3. The quantitative decolorization test was performed in MSM liquid media which were amended by three different pH variations: 6, 7 and 8; Three different azo concentrations which were: 600, 800 dan 1000 ppm and three different salinity of media which were: 0 %, 5%, and 10%. The optimum conditions for isolate to degrade azo dye were shown by isolate DH2 at pH 6, salinity 5% and azo concentration of 600 ppm which was 49.64%. Based on 16S rRNA gene, it was known that DH2 was identified to close to Stenotrophomonas pavanii strain LMG 25348 with homology of 97%.
Identification of Blast Resistance Genes in Fifteen Rice Accessions (Oryza sativa L.) from North Sumatera
Authors: Hannum Saleha , Hasibuan Ummu Kulsum , Sinaga Riyanto , Wahyuningsih Hesti 
Published: July 2019

AbstractOne challenge in improving rice productivity is the occurrence of blast disease caused byPyricularia grisea. Development of current disease is affected by several factors, one of which is an expression of resistance gene towards blast by rice systemic defense. In order to construct blast-resistant variety, research has been conducted to identify resistance-controlling gene against blast in fifteen rice accessions native to North Sumatera. Identification of blast resistance gene performed using PCR amplification of rice genome with six specific primers:Pi-d2, Pupl, Pi-ta2, Pi-37, Pi-z, andPib.The results showed that genes,Pi-d2, Pi-z, andPibwere present on fifteen rice accessions, genePup1positively located in fourteen accessions (93%),Pi-ta2in thirteen accessions (87%), andPi-37in seven accessions (47%). Rice accessions with six blast resistance genes detected were:Ampari 3, Bagendi 1, Bagendi 3, Martabe 3, Siporang 2, andSilatian 2.The least number of genes were detected from two accesions namely:Ampari 2andSirara 2, with only four resistance genes.
Effect of Turmeric Rhizome Extract (Curcuma longa L.) on Kidney Histology of Preeclampsia Rats (Rattus norvegicus L.)
Authors: Ilyas S , Hutahaean S , Elimasni  , Panjaitan S R N 
Published: July 2019

Abstract
Preeclampsia (PE) is second death in pregnancy in Indonesia. This study aims to determine the effect of turmeric extract (Curcuma longa L.) on renal necrosis, narrowing of the proximal tubulus, renal weight and changed of renal color of rats. This study used a type of analytic study with an experimental quasi design in pregnant female rats (Rattus norvegicus) consisting of four treatments with six replications each. The study consisted of a negative control group (K-) with only feed given ad libitum, positive control (K +) in injection of LPS intervena with a dose of 0.2 / kgBW, and 2 group giving turmeric extract (Curcuma longa L.) with doses of 400 mg / kgBW (P1) and 600 mg / kgBW (P2). Rats (Rattus norvegicus) are disectedto take the kidneys. Kidneys are made into a preservative preparation with the paraffin method and staining Hematoxylin-Eosin (HE). The results of renal morphology observations showed a change in color in the treatment group P2. The histological features of the kidneys in the proximal tubulus showed a decrease in P1 compared to the P2 group and the percentage of renal necrosis in P2 had a significant change compared to the positive control group (K+).Extract of Turmericrhizome (Curcuma longa L.) is proven to be able to maintain and improve the structure of cells of the kidneys in the Rats preeclampsia model.
Antioxidant Activity of Haramounting Leaf Ethanol Extract (Rhodomytrus tomentosa) In Preventing Heart Damage of Mice (Mus musculus L.) after exposure to Electronic Cigarette
Authors: Ilyas S , Tanjung R S , Hutahaean S , Tanjung M , Elimasni  , Jamilah I , Murdela F 
Published: July 2019

Abstract
Cigarette smoke is one factor that increases free radicals in the blood will affect liver health in the body. The purpose of this study is to find outthe effect of giving leaves ethanol extract haramounting to histology of male mice liver exposed to electric cigarettes. The design of this study used Completely Randomized Design (CRD) consisting of + Group, - Group and three treatments of Haramounting Leaf Ethanol Extract (Rhodomyrtus tomentosa (Aiton) Hassk.) for 30 days at a dose of 100 mg/kg BW Body Weight, 200 mg/kg BW and 300 mg/kg BW for treament use of five replications. Liver organ preparations were made using paraffin block method and Hematoxylin Erlich-Eosin (HE) staining. The results of the study show on histological observations The liver has a tendency that electric cigarette smoke can cause damage in liver cells. The results of this study indicate that ethanol extract leaves of Haramounting after exposure to eletric cigarette smoke can effectively regenerate damaged liver cells.
Immunostimulatory Activity from Pirdot Leaves Ethanolic Extract (Saurauia vulcani Korth.) in Rats (Rattus norvegicus)
Authors: Sinaga Erlintan , Ilyas Syafruddin , Hutahaean Salomo , Sitorus Panal 
Published: July 2019

Abstract
Erythrocyte, lymphocyte quantities and spleen histology were observed to investigate the immunostimulatory effect Pirdor leaves ethanolic extract (Saurauia vulcani Korth.) (EES) in white rats (Rattus norvegicus L.) -induced with sheep red blood cell (SRBC) as antigen. Experimental design used in this study was Complete Randomized Design. Twenty four rats were classified into four groups: a control group fed with distilled water (G0), a group treated with sheep red blood cell (SRBC) (G1), a group treated with ethanol extract of pirdot leaves (G2), and a group treated with ethanol extract of pirdot leaves + SRBC (G3) for 31 days. Blood was withrawnThen the red blood cell in white rats were sized by using ABX micros 60. Spleen of white rats were taken and stained by using haematoxylin and eosin to visualize lymphoid follicles. Data were analyzed by one-way ANOVA at 5% significance level. Significant differences were observed in total erythrocytes and spleen histological image which showed a prospect of EES as immunostimulant.
The Ability of Fungi Isolated from Landfill in Decolorization of Liquid Waste of Batik Industry
Authors: Lubis M A Y , Munir E , Nurtjahja K 
Published: July 2019

Abstract
Isolation of soil fungi from landfill in Medan, North Sumatera, Indonesia was conducted. The aim was to investigate the fungal that potential to decolorize waste liquid batik home industry. Results showed that twenty-three species of filamentous fungi were successfuly isolated. All of the isolates were cultured in minimum salt medium containing waste liquid batik at concentration 25, 50, and 75%. At 75% waste, only 4 fungal isolates (Ys02, Ys13, Ys14, and Ys21) showed growth response. Cultured of the fungal isolates in minimum salt medium with 25% waste + 0.5% glucose were found three fungal isolates (Ys02, Ys14, and Ys21). Whereas, medium with 25% waste with no glucose were found only two fungal isolates (Ys14, and Ys21).
Keratinolytic fungi isolated from Asam Kumbang Crocodile Breeding Farm, Medan, North Sumatra
Authors: Mamangkey J , Suryanto D , Munir E , Mustopa A Z 
Published: July 2019

Abstract
Hydrolysis of keratin waste by fungi is an alternative biotechnology for recycling and valorization by utilizing its keratinolytic activities. The purpose of this study was to isolate the keratinolytic fungi and to test the degradation ability of chicken feather keratin. Crocodile feces and soil samples were collected from crocodile breeding farm in Asam Kumbang, North Sumatera. Casein and keratin of basal feather agar of 1% was used to isolate keratinolytic fungi. Fungal isolate was grown in feather meal broth incubated at 28°C and shake at 180 rpm using shaking orbital. Remain chicken feather was weighted after application of keratinolytic fungi. After 4 days of incubation two fungi showed to have clear zone around their colony. THB7 was found to have relatively high hydrolysis zone in casein, while FB4 degraded more keratin in keratin agar. Most feather was degraded in 10, 12, and 16 days in THB7, FB4, and THB4 application respectively. THB4 showed to degrade feather to 1.6 g, while FB3 and FB4 remained feather to 2 and 3.4 of 10 g respectively. Further study includes molecular identification, characterization and keratinase production should be done.