bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses
Authors:   The CRUK IMAXT Grand Challenge Team, O’Flanagan Ciara H. , Campbell Kieran R. , Zhang Allen W. , Kabeer Farhia , Lim Jamie L. P. , Biele Justina , Eirew Peter , Lai Daniel , McPherson Andrew , Kong Esther , Bates Cherie , Borkowski Kelly , Wiens Matt , Hewitson Brittany , Hopkins James , Pham Jenifer , Ceglia Nicholas , Moore Richard , Mungall Andrew J. , McAlpine Jessica N. , Shah Sohrab P. , Aparicio Samuel 
Published: October 2019

Abstract

Background
Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying complex biological systems, such as tumor heterogeneity and tissue microenvironments. However, the sources of technical and biological variation in primary solid tumor tissues and patient-derived mouse xenografts for scRNA-seq are not well understood.

Results
We use low temperature (6 °C) protease and collagenase (37 °C) to identify the transcriptional signatures associated with tissue dissociation across a diverse scRNA-seq dataset comprising 155,165 cells from patient cancer tissues, patient-derived breast cancer xenografts, and cancer cell lines. We observe substantial variation in standard quality control metrics of cell viability across conditions and tissues. From the contrast between tissue protease dissociation at 37 °C or 6 °C, we observe that collagenase digestion results in a stress response. We derive a core gene set of 512 heat shock and stress response genes, including FOS and JUN, induced by collagenase (37 °C), which are minimized by dissociation with a cold active protease (6 °C). While induction of these genes was highly conserved across all cell types, cell type-specific responses to collagenase digestion were observed in patient tissues.

Conclusions
The method and conditions of tumor dissociation influence cell yield and transcriptome state and are both tissue- and cell-type dependent. Interpretation of stress pathway expression differences in cancer single-cell studies, including components of surface immune recognition such as MHC class I, may be especially confounded. We define a core set of 512 genes that can assist with the identification of such effects in dissociated scRNA-seq experiments.
Pretreatment Vitamin D Deficiency Is Associated With Impaired Progression-Free and Overall Survival in Hodgkin Lymphoma
Authors: Borchmann Sven , Cirillo Melita , Goergen Helen , Meder Lydia , Sasse Stephanie , Kreissl Stefanie , Bröckelmann Paul Jan , von Tresckow Bastian , Fuchs Michael , Ullrich Roland Tillmann , Engert Andreas 
Published: October 2019

PURPOSE Vitamin D deficiency is described as a modifiable risk factor for the incidence of and mortality in many common cancers; however, data in Hodgkin lymphoma (HL) are lacking. PATIENTS AND METHODS We thus performed a study measuring pretreatment vitamin D levels in prospectively treated patients with HL and correlated this with clinical outcomes. A total of 351 patients from the German Hodgkin Study Group clinical trials (HD7, HD8, and HD9) were included. RESULTS Fifty percent of patients were vitamin D deficient (< 30 nmol/L) before planned chemotherapy. Pretreatment vitamin D deficiency was more common in relapsed/refractory patients than matched relapse-free controls (median baseline vitamin D, 21.4 nmol/L v 35.5 nmol/L; proportion with vitamin D deficiency, 68% v 41%; P < .001). Vitamin D–deficient patients had impaired progression-free survival (10-year difference, 17.6%; 95% CI, 6.9% to 28.4%; hazard ratio, 2.13; 95% CI, 1.84 to 2.48; P < .001) and overall survival (10-year difference, 11.1%; 95% CI, 2.1% to 20.2%; hazard ratio, 1.82; 95% CI, 1.53 to 2.15; P < .001), consistent across trials and treatment groups. We demonstrated that vitamin D status is an independent predictor of outcome and hypothesized that vitamin D status might be important for the chemosensitivity of HL. We subsequently performed experiments supplementing physiologic doses of vitamin D (calcitriol) to cultured HL cell lines and demonstrated increased antiproliferative effects in combination with chemotherapy. In an HL-xenograft animal model, we showed that supplemental vitamin D (dietary supplement, cholecalciferol) improves the chemosensitivity of tumors by reducing the rate of tumor growth compared with vitamin D or chemotherapy alone. CONCLUSION On the basis of our clinical and preclinical findings, we encourage that vitamin D screening and replacement be incorporated into future randomized clinical trials to properly clarify the role of vitamin D replacement therapy in HL.
Painting Prophecy
Authors: Cohen-Aponte Ananda , Diaz Ella Maria 
Published: October 2019

AbstractThis article examines the work of the Chicana artist Sandy Rodriguez, who created the Codex Rodriguez-Mondragón, an ongoing project begun in 2017 that consists of botanical illustrations and large-scale maps of California and northern Mexico. Rodriguez’s Codex draws on pre-Hispanic, colonial, and Chicana/o/x antecedents, most notably the Florentine Codex (sixteenth century) and the Chicana/o/x codices of the early 1990s, produced in the context of the quincentenary of Columbus’s voyage. This article posits Rodriguez’s Codex as a polyphonic text that exceeds both the linguistic of the literary and the visual of the artistic, drawing on a multiplicity of sources, both historical and contemporary, visual and textual, oral and aural, in her mapping of California’s land and history. The Codex Rodriguez-Mondragón collapses precolonial, colonial, and contemporary histories to underscore continuities between the ruptures of conquest and our dangerous geopolitical moment.
Trauma and the African Animist Imaginary in Aminatta Forna’s The Memory of Love and Delia Jarrett-Macauley’s Moses, Citizen, and Me
Authors: Topper Ryan 
Published: October 2019

Abstract
This essay intervenes in debates surrounding trauma theory and postcolonial studies, tracing how forms of African animism can lead to a decolonized discourse of trauma. Taking the postcolonial critique of trauma theory’s Eurocentrism as a point of departure, the essay focuses on two contemporary novels of the African diaspora: Aminatta Forna’s The Memory of Love and Delia Jarrett-Macauley’s Moses, Citizen, and Me. Narrating local forms of survival in post–civil war Sierra Leone, these novels use animist modes of consciousness to theorize the collective trauma of, and envision political futures for, Sierra Leone. Forna’s writing is emblematic of realism, while Jarrett-Macauley’s is an example of animist realism. Both novels are united, however, by an animism at the level of narrative process, drawing on the spirit world and possession rituals to counter therapeutic and humanitarian ideologies.
Refusing Historical Amnesia
Authors: Stamant Nicole 
Published: October 2019

Abstract
Performing what Michele Elam calls “a refusal of historical amnesia,” Danzy Senna and Emily Raboteau expose how social justice and hospitality are constructed in and around what Pierre Nora calls lieux de mémoire. Engaging particular sites of memory in the American South—places with national and personal significance—Raboteau and Senna negotiate and interrogate the interstitial spaces of racial ambiguity, liminality, and invisibility as they uncover different modes of commemoration and fend off historical forgetting. Writing about their experiences as biracial African Americans, Raboteau and Senna show readers how memorialization of black southern experience connects with communal or inherited familial memories. Their considerations of memory, and the attendant concerns about subjectivity and forgetting, demonstrate the central place of testimony to mnemonic restitution. In so doing, they also expose new ways to engage trauma: through the affect of what Lauren Berlant describes as “crisis ordinariness.”
The dive performance of immature king penguins following their annual molt suggests physiological constraints
Authors: Enstipp Manfred R. , Bost Charles-André , Le Bohec Céline , Bost Caroline , Laesser Robin , Le Maho Yvon , Weimerskirch Henri , Handrich Yves 
Published: October 2019

ABSTRACT
Like all birds, penguins undergo periodic molt, during which they replace old feathers. However, unlike other birds, penguins replace their entire plumage within a short period while fasting ashore. During molt, king penguins (Aptenodytes patagonicus) lose half of their initial body mass, most importantly their insulating subcutaneous fat and half of their pectoral muscle mass. The latter might challenge their capacity to generate and sustain a sufficient mechanical power output to swim to distant food sources and propel themselves to great depth for successful prey capture. To investigate the effects of the annual molt fast on their dive/foraging performance, we studied various dive/foraging parameters and peripheral temperature patterns in immature king penguins across two molt cycles, after birds had spent their first and second year at sea, using implanted data-loggers. We found that the dive/foraging performance of immature king penguins was significantly reduced during post-molt foraging trips. Dive and bottom duration for a given depth were shorter during post-molt and post-dive surface interval duration was longer, reducing overall dive efficiency and underwater foraging time. We attribute this decline to the severe physiological changes that birds undergo during their annual molt. Peripheral temperature patterns differed greatly between pre- and post-molt trips, indicating the loss of the insulating subcutaneous fat layer during molt. Peripheral perfusion, as inferred from peripheral temperature, was restricted to short periods at night during pre-molt but occurred throughout extended periods during post-molt, reflecting the need to rapidly deposit an insulating fat layer during the latter period.
СОЦИАЛЬНЫЙ КАПИТАЛ, МЕЖЛИЧНОСТНОЕ ОТЧУЖДЕНИЕ И ПСИХИЧЕСКОЕ ЗДОРОВЬЕ НАСЕЛЕНИЯ РЕГИОНА
Authors: Смолева Елена 
Published: October 2019

В статье представлены результаты исследования влияния социального капитала на состояние психического здоровья населения региона. Социальный капитал рассматривается через показатели межличностного взаимодействия и вхождения в социальные сети. Эмпирическое исследование проводилось с помощью социологического опроса населения Вологодской области с использованием модели многоступенчатой районированной выборки с квотным отбором единиц наблюдения. При помощи факторного анализа выявлено 5 латентных переменных, характеризующих включенность в социальные сети населения региона, выявлены варианты структуры социальных сетей. С помощью кластерного анализа на основе выделенных латентных переменных жители области разделились на три группы, которые различаются и по субъективным оценкам здоровья. Определено, что межличностное отчуждение в виде малоактивного общения и отсутствие социальной поддержки связано с более частными проявлениями негативных психических состояний в виде тревоги, депрессии, невроза.
A GH13 glycoside phosphorylase with unknown substrate specificity from Corallococcus coralloides
Authors: Franceus Jorick , Desmet Tom 
Published: October 2019

Abstract
Glycoside phosphorylases in subfamily GH13_18 of the carbohydrate-active enzyme database CAZy catalyse the reversible phosphorolysis of α-glycosidic bonds. They contribute to a more energy-efficient metabolism in vivo, and can be applied for the synthesis of valuable glucosides, sugars or sugar phosphates in vitro. Continuing our efforts to uncover new phosphorylase specificities, we identified an enzyme from the myxobacterium Corallococcus coralloides DSM 2259 that does not feature the signature sequence patterns of previously characterised phosphorylases. The enzyme was recombinantly expressed and subjected to substrate screening. Although it was confirmed that the Corallococcus phosphorylase does not have the same substrate specificity as other phoshorylases from subfamily GH13_18, its true natural substrate remains a mystery for now. Myxobacteria have been widely investigated as producers of numerous bioactive secondary metabolites for decades, but little research has been conducted on myxobacterial proteins. The present study exemplifies the untapped metabolic activities and functional diversity that these fascinating organisms may have left to show.
Post-Quantum Crystography System for Secure Electronic Voting
Authors: Junior Gabriel Arome , Alese Boniface Kayode , Adetunmbi Adebayo Olusola , Adewale Olumide Sunday , Sarumi Oluwafemi Abimbola 
Published: October 2019

Abstract
Security (privacy, confidentiality and integrity) of pre-electoral, electoral and post electoral phases of the electioneering process is fundamental to the success of Electronic Voting (E-Voting) Systems. Crystography, which is the combination of cryptography and steganography could be a fitting ‘tool kit’ for enhancing the security of sensitive election-related information transmitted over public networks, thereby also ensuring free, fair and credible election/voting. Most of the existing secure e-voting systems are based on public key cryptographic schemes like RSA and Elliptic Curve Cryptography (ECC), whose security depends on the difficulty of solving Integer Factorization Problem (IFP) and Discrete Logarithm problem (DLP) respectively. However, techniques for solving IFP and DLP problems, improves continually. One of such is the quantum algorithm discovered by Peter Shor in 1994, which can solve both IFP and DLP problems in polynomial time. Consequently, the existence of quantum computers in the range of 1000 bits would spell doom to systems based on those problems. This paper presents the development of a new crystographic system that combines Post Quantum Cryptography with steganography to ensure that the security of e-voting is maintained both in classical computing era as well as post-quantum computing era. Our experiments’ results shows that our proposed system performed better than existing ones.
Ramp secret sharing with cheater identification in presence of rushing cheaters
Authors: Pramanik Jyotirmoy , Adhikari Avishek 
Published: October 2019

Abstract
Secret sharing allows one to share a piece of information among n participants in a way that only qualified subsets of participants can recover the secret whereas others cannot.
Some of these participants involved may, however, want to forge their shares of the secret(s) in order to cheat other participants.
Various cheater identifiable techniques have been devised in order to identify such cheaters in secret sharing schemes.
On the other hand, Ramp secret sharing schemes are a practically efficient variant of usual secret sharing schemes with reduced share size and some loss in security.
Ramp secret sharing schemes have many applications in secure information storage, information-theoretic private information retrieval and secret image sharing due to producing relatively smaller shares.
However, to the best of our knowledge, there does not exist any cheater identifiable ramp secret sharing scheme.
In this paper we define the security model for cheater identifiable ramp secret sharing schemes and provide two constructions for cheater identifiable ramp secret sharing schemes.
In addition, the second construction is secure against rushing cheaters who are allowed to submit their shares during secret reconstruction after observing other participants’ responses in one round.
Also, we do not make any computational assumptions for the cheaters, i.e., cheaters may be equipped with unlimited time and resources, yet, the cheating probability would be bounded above by a very small positive number.
Water Oxidation Catalysts from Waste Metal Resources: A Facile Metal-Organic Electrochemical Approach
Authors: Lublow Michael , Schedel-Niedrig Thomas 
Published: October 2019

Abstract
A fabrication route is presented by a novel metal-organic electrochemical approach, allowing facile preparation of electrocatalytic metal and metal oxide thin films from solid metals at room temperature: divalent transition metals such as iron, nickel and cobalt can be deposited as amorphous oxyhydroxide films. Among them, nickel- and cobalt-prepared samples are showing high activity as water oxidation catalysts in alkaline electrolytes. The applicability to waste metal material is exemplified using one of the most abundant waste metal alloys, i.e. steel: with a suitable multi-layer architecture, comprising, a large surface-area iron oxyhydroxide core-geometry and a steel-derived catalytic overlayer, the overpotential for oxygen evolution (at 10 mA cm−2) could be improved to only 370 mV. Chemical analysis is provided to elucidate the reaction pathway, encompassing metal halogenation, formation of meta-stable metal-organic intermediates or direct electrochemical reduction, respectively. Structural peculiarities of the derived films are finally demonstrated upon development of a photoactive nickel oxyhydroxide/silicon junction realizing a photocurrent of 1 mA cm−2 at the thermodynamic potential for oxygen evolution and short-term stabilization in the range of several hours.
Variables de la digesta en rumen de novillos y toros de lidia con diferencias en la procedencia y estación del año en las corridas taurinas del estado de Tlaxcala, México
Authors: Figueroa Sara , Hernandez Luz M , Garcia Guillermina 
Published: October 2019

El objetivo del estudio fue evaluar el peso corporal (PC), la edad y las variables digestivas en el rumen de los animales lidiados en eventos taurinos del estado de Tlaxcala durante un año. En veinte eventos taurinos se registró la procedencia (ganadería de Tlaxcala [GL]; ganadería fuera del estado de Tlaxcala [GF]) y la época de estudio (sequía y lluvia), registrándose las variables: PC, edad (dentición), peso de la digesta del rumen, contenido de la fibra detergente neutro (FDN), pH, ácidos grasos volátiles (AGV), conteo de protozoarios en rumen y la presencia de abscesos hepáticos. Los toros tuvieron mayor PC que los novillos (456 vs 361, p ≤ 0.05). En la estación de sequía se registró mayor PC (432 vs 405, p ≤ 0.05) que durante la estación de lluvia; presentando interacción estadística entre la clasificación (novillo o toro) y la estación (p ≤ 0.05). Los animales del GF tuvieron mayor contenido de la digesta que el GL (8.1 vs 7.25%, p ≤ 0.05). El pH ruminal fue menor en los novillos que en los toros (5.86 vs 6.54, p ≤ 0.05), al igual que el contenido de FDN (61.2 vs 64.7, p < 0.05). En conclusión, los novillos presentaron mayores problemas de acidosis ruminal que los toros. El reglamento taurino, con base al peso y edad de los animales, no es aplicado correctamente por las autoridades.
Population genetics of lepidopteran (noctuidae) collected on transgenic and non-transgenic maize in Mexico
Authors: Alvarado-Canche Carmen Natividad , Castillo Reyes Francisco , González-Vázquez Víctor Manuel , Garcia-Martinez Oswaldo , Aguirre-Uribe Luis Alberto , Tiscareño-Iracheta Miguel Angel , Aguilar-González Cristóbal Noé , Rodríguez-Herrera Raúl 
Published: October 2019

Allele frequency of the resistance of the gene Onb3GalT5 to Cry proteins was analyzed in insect populations (Helicoverpa zea and Heliothis virescens), collected from maize transgenic and non-transgenic plants. The genetic parameters estimated in these populations were allele and genotype frequency, genetic balance and Wright statistics (Fis, Fit and Fst). Statistical analyses were performed using the package Genepop (4.0.10). Results showed that from the DNA of H. virescens, collected on non-transgenic maize, a higher number of bands were amplified in a range from 250 bp to 600 bp. In contrast, the bands amplified from insects collected on transgenic maize were in the range of 250 bp-500 bp, being the 400 bp band in both cases the most frequent, while in the DNA of Helicoverpa zea, collected on transgenic maize, three bands (350 bp, 400 bp and 450 bp) were amplified, and in H. zea, collected on non-transgenic maize, only two bands (350 bp and 400 bp) were observed. Genetic diversity in insect populations was higher than within these same populations.
Adaptación y validación de la Escala Orden, Bullicio y Confusión para padres de familia mexicanos
Authors: Sánchez-Mondragón Gabriela , Flores Herrera Luz María 
Published: October 2019

Las condiciones socio-físicas del entorno familiar desempeñan un papel fundamental en el desarrollo infantil y los procesos que se generan dentro del hogar. En los hogares desordenados o caóticos, se han encontrado resultados negativos en el desarrollo infantil y la conducta parental, por lo que es necesario contar con instrumentos para evaluar las características de este tipo de hogares. El objetivo de este estudio fue adaptar y validar la Escala de Orden, Bullicio y Confusión (CHAOS, por sus siglas en inglés) en padres de familia mexicanos. Participaron mujeres y hombres de 19 a 49 años (M = 31.0, DE = 6.31). Se reporta una versión de la escala con nueve reactivos cuya validez y confiabilidad es adecuada (α = 0.72). Se encontró apoyo empírico para la estructura de tres factores (confusión, orden y ausencia de implementación de rutinas) tras la ejecución del análisis factorial confirmatorio (Xi2= 30.097, p = 0.18, RMSEA = 0.051).
Risk of salinization and sodification of irrigated soils in the Lerma River basin
Authors: Pérez-Díaz José Pedro , Ortega-Escobar Héctor Manuel , Ramírez-Ayala Carlos , Flores-Magdaleno Héctor , Sánchez-Bernal Edgar Iván , Can-Chulim Álvaro , Mancilla-Villa Oscar Raúl 
Published: October 2019

Factors as agricultural development, the presence of important industrial centers and a population of more than 15 million inhabitants in the Lerma River basin generate wastewaters which are poured into the Lerma River course. This study was carried out to evaluate the quality of the Lerma River water and to estimate the risk of salinization and sodification of the irrigated soils in the basin. Electrical conductivity (EC) was less than 1.252 dS m-1 and water was classified as bicarbonated-sodic, with a medium-low risk of salinity-sodicity (C2-S1, C3-S1). The minimum and maximum sodium adsorption ratio (SAR) values were 2.80 mmol L-1 and 7.63 mmol L-1, and the estimated maximum exchangeable sodium percentage (ESP) was 11.42. Based on the results of this research, the Lerma River water may induce salinization and sodification processes in the irrigated soils.
Evaluación de tres compuestos fenólicos como blancos potenciales en el control de la antracnosis
Authors: Cruz Vázquez Julieta Karina , Contreras García Laura Itzel , Ávila Serrano Narcizo Ysac , Bernabé Pineda Margarita , Ruiz Francisco Gumaro Ruiz 
Published: October 2019

La búsqueda de alternativas para el control de la antracnosis es determinante para prevenir el desarrollo del agente causal en los cultivos que son susceptibles a dicha enfermedad. Se realizaron análisis de sensibilidad in vitro a una cepa de Colletotrichum gloeosporioides identificada con claves taxonómicas y técnicas moleculares con tres compuestos fenólicos (catecol, orcinol y resorcinol). Para evaluar la efectividad de cada compuesto se determinó el área de crecimiento micelial en milímetros cuadrados (mm2) con el software ImageJ®, validándolo con un análisis estadístico con el software SAS®. En los bioensayos el resorcinol a una concentración de 200 partes por millón (ppm) presentó menor acción de inhibición de crecimiento micelial a diferencia del catecol y orcinol. Los resultados mostrados sugieren al catecol y orcinol como posibles blancos de estudio como ingredientes activos de los fungicidas usados para el control del fitopatógeno asociado a la antracnosis en frutos de importancia económica.