bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns in On-time, Daily Submission of a Short Web-Based Personal Behavior Survey in a Longitudinal Women's Health Study
Authors: Crowder Hannah R. , Brown Sarah E. , Stennett Christina A. , Johnston Elizabeth , Wnorowski Amelia M. , Mark Katrina S. , Brotman Rebecca M. 
Published: March 2019

Abstract
We evaluated compliance with submitting a short Web-based personal behavior survey daily during a 10-week study (n = 52 women/3419 diaries). Time-stamped forms revealed that 50% of diaries were submitted within 24 hours of the email prompt, and 19% were missing or submitted more than 3 days late. Late submissions may affect data quality.
Case Report: Iridocorneal Endothelial Syndrome Progression Documented by Anterior Segment Optical Coherence Tomography
Authors: Hillard Jacob G. 
Published: March 2019

SIGNIFICANCE
Iridocorneal endothelial (ICE) syndrome is characterized by a difficult-to-see membrane of ectopic corneal endothelial cells, called an ICE membrane. This report demonstrates that ICE membranes are detectable by anterior segment optical coherence tomography (AS-OCT) and highlights the benefits of using AS-OCT in ICE syndrome.

PURPOSE
A case of ICE syndrome is reported to highlight the utility of AS-OCT in the characterization and diagnosis of the disease.

CASE REPORT
A 65-year-old African American man was diagnosed as having the Chandler variant of ICE syndrome of the left eye. He was treated topically for corneal edema. Anterior segment optical coherence tomography and anterior segment photography were used to aid in the diagnosis and document an ICE membrane and multiple peripheral anterior synechiae. Further examinations chronicled the progression of a peripheral anterior synechia. At first, only iridocorneal touch (sparing the trabecular meshwork) was present, but later, full apposition to the trabecular meshwork developed. The patient was diagnosed as having pre-perimetric glaucoma in the left eye and is being treated topically to reduce his intraocular pressure. The right eye remains unremarkable.

CONCLUSIONS
Anterior segment optical coherence tomography can support the diagnosis of ICE syndrome by visualizing ICE membranes and differentiating between true peripheral anterior synechiae and iridocorneal touch that does not block the trabecular meshwork. Chronicling ICE membranes longitudinally with AS-OCT will enhance understanding of disease progression. By correlating membranes features (such as location and extent) and patient outcomes, AS-OCT–based ICE membrane classifications can be developed to improve the management and prognosis of ICE syndrome.
Calmer: a robot for managing acute pain effectively in preterm infants in the neonatal intensive care unit
Authors: Holsti Liisa , MacLean Karon , Oberlander Timothy , Synnes Anne , Brant Rollin 
Published: March 2019

Abstract

Introduction:
For preterm infants in the neonatal intensive care unit, early exposure to repeated procedural pain is associated with negative effects on the brain. Skin-to-skin contact with parents has pain-mitigating properties, but parents may not always be available during procedures. Calmer, a robotic device that simulates key pain-reducing components of skin-to-skin contact, including heart beat sounds, breathing motion, and touch, was developed to augment clinical pain management.

Objective:
Our objective was to evaluate the initial efficacy of Calmer for mitigating pain in preterm infants. We hypothesized that, compared to babies who received a human touch–based treatment, facilitated tucking, infants on Calmer would have lower behavioural and physiological pain indices during a single blood test required for clinical care.

Methods:
Forty-nine preterm infants, born between 27 and 36 weeks of gestational age, were randomized either to facilitated tucking or Calmer treatment. Differences between groups in changes across 4 procedure phases (baseline 1, baseline 2, poke, and recovery) were evaluated using (1) the Behavioral Indicators of Infant Pain scored by blind coders from bedside videotape and (2) heart rate and heart rate variability continuously recorded from a single-lead surface ECG (lead II) (Biopac, Canada) sampled at 1000 Hz using a specially adapted portable computer system and processed using Mindware.

Results:
No significant differences were found between groups on any outcome measures.

Conclusion:
Calmer provided similar treatment efficacy to a human touch–based treatment. More research is needed to determine effects of Calmer for stress reduction in preterm infants in the neonatal intensive care unit over longer periods.
The Surface-Volume Coefficient of the Superficial and Deep Facial Fat Compartments: A Cadaveric Three-Dimensional Volumetric Analysis
Authors: Cotofana Sebastian , Koban Konstantin C. , Konstantin Frank , Green Jeremy B. , Etzel Lucas , Giunta Riccardo E. , Schenck Thilo L. 
Published: March 2019

Background:
Soft-tissue filler injections for the treatment of facial aging can result in different skin surface effects depending on the targeted facial fat compartment and fascial plane. This work investigates the tissue response of defined amounts of soft-tissue filler material injected into superficial and deep facial fat compartments by means of the calculation of the surface-volume coefficient.

Methods:
Four fresh frozen cephalic specimens obtained from human donors (three female and one male; mean age, 74.96 ± 22.6 years; mean body mass index, 21.82 ± 6.3 kg/m2) were studied. The superficial and deep lateral forehead compartments, deep temporal fat pad, sub–orbicularis oculi fat compartment, and deep medial cheek fat compartment were injected with aliquots of 0.1 cc of contrast-enhanced material and scanned using three-dimensional surface imaging, resulting in a total of 226 injection and scanning procedures.

Results:
The sub–orbicularis oculi fat compartment revealed the highest correlation coefficient (r

p
= 0.992; p < 0.001) and the highest surface-volume coefficient (0.94). The compartment with the lowest tissue response was the deep medial cheek fat compartment (r

p
= 0.745; p < 0.001; surface-volume coefficient = 0.29), followed by the deep lateral forehead compartment (r

p
= 0.814; p < 0.001; surface-volume coefficient = 0.68), superficial lateral forehead compartment (r

p
= 0.824; p < 0.001; surface-volume coefficient = 0.74), and deep temporal fat pad (r

p
= 0.947; p < 0.001; overall surface-volume coefficient = 0.64).

Conclusions:
These results, confirmatory in their nature to current injection strategies, provide evidence for the validity and reliability of the surface-volume coefficient. Injection procedures should be targeted in terms of facial fat compartments and fascial planes for a desired aesthetic outcome, as each fat compartment and fascial plane has unique tissue responses to injected soft-tissue fillers.
Synergistic Effect of Adipose-Derived Stem Cells and Fat Graft on Wrinkles in Aged Mice
Authors: Kim Kikap , Fan Yingfang , Lin Guang , Park Yoon Kyung , Pak Chang Sik , Jeong Jae Hoon , Kim Sukwha 
Published: March 2019

Background:
The authors investigated the synergistic effects of adipose-derived stem cells and fat graft on skin wrinkles in a nude mouse model of chronologic aging.

Methods:
After 50 weeks of chronologic aging, 44 female BALB/c nude mice were classified into four groups: (1) negative control, (2) mice injected subcutaneously with fat on the back skin (0.5 cm3), (3) mice injected with adipose-derived stem cells (1 × 105 cells in 0.5 cm3 Hanks balanced salt solution), and (4) mice injected with both fat (0.5 cm3) and adipose-derived stem cells (1 × 105 cells in 0.5 cm3 Hanks balanced salt solution). The degree of wrinkling was evaluated using replica analysis, and skin biopsies were performed after 4 weeks. The dermal thickness and density of collagen were determined. Type I procollagen and matrix metalloproteinase levels were determined using real-time polymerase chain reaction and Western blot analysis. Tropoelastin, fibrillin-1, and CD31 levels were evaluated using immunohistochemistry.

Results:
Based on the total wrinkle area, there was significant wrinkle reduction in the fat-treated and adipose-derived stem cell with fat–treated groups. Type I procollagen mRNA and collagen levels were significantly higher in the adipose-derived stem cell with fat–treated group than in the adipose-derived stem cell–treated and the fat-treated groups. In addition, the adipose-derived stem cells with fat graft group exhibited significantly higher CD31 expression level than the adipose-derived stem cell–treated and the fat-treated groups.

Conclusion:
Both adipose-derived stem cells and fat graft have a wrinkle-reducing effect and synergistically affect collagen synthesis and neovascularization.
The Role of Deferoxamine in Irradiated Breast Reconstruction: A Study of Oncologic Safety
Authors: Lynn Jeremy V. , Urlaub Kevin M. , Ranganathan Kavitha , Donneys Alexis , Nelson Noah S. , Subramanian Chitra , Cohen Mark S. , Buchman Steven R. 
Published: March 2019

Background:Radiotherapy plays an essential role in the oncologic management of breast cancer. However, patients who undergo radiotherapy experience significantly more wound complications during the reconstructive process. Deferoxamine has immense potential to up-regulate angiogenesis and improve reconstructive outcomes. The purpose of this study was to determine the impact of deferoxamine on breast cancer cell proliferation in vitro, to delineate oncologic safety concerns regarding the use of deferoxamine as a regenerative therapeutic.Methods:The dose-dependent effect of radiation and deferoxamine on two triple-negative breast cancer cell lines (MDA-MB-231 and MDA-MB-468) was determined by means of MTS (percentage cell viability) and tumorsphere (sphere number) analysis. Radiation therapy and deferoxamine were delivered both individually and in combination, and all experiments were completed in triplicate. Intracellular iron, nuclear factor-κB localization, and apoptosis/necrosis assays were performed to delineate mechanism. Analysis of variance statistical analysis was performed using SPSS (p< 0.05).Results:For both cell lines, percentage viability and sphere number significantly decreased following exposure to 10 Gy of radiation. Surprisingly, the administration of 25 µM deferoxamine also significantly decreased each metric. The administration of deferoxamine (100 µM) in combination with radiation (10 Gy) resulted in significantly reduced percentage viability and sphere number compared with the administration of radiation alone. Deferoxamine treatment decreased intracellular iron, suppressed nuclear factor-κB activation, and induced apoptosis.Conclusion:Radiation and deferoxamine significantly decrease breast cancer proliferation when delivered independently and in combination, suggesting deferoxamine may be safely used to facilitate improved reconstructive outcomes among triple-negative breast cancer survivors.CLINICAL QUESTION/LEVEL OF EVIDENCE:Therapeutic, V.
Managing Missing Data in the Hospital Survey on Patient Safety Culture: A Simulation Study
Authors: Boussat Bastien , François Olivier , Viotti Julien , Seigneurin Arnaud , Giai Joris , François Patrice , Labarère José 
Published: March 2019

Background
Case-wise analysis is advocated for the Hospital Survey on Patient Safety culture (HSOPS).

Objectives
Through a computer-intensive simulation study, we aimed to evaluate the accuracy of various imputation methods in managing missing data in the HSOPS.

Methods
Using the original data from a cross-sectional survey of 5064 employees at a single university hospital in France, we produced simulation data on two levels. First, we resampled 1000 completed data based on the original 3045 complete responses using a bootstrap procedure. Second, missing values were simulated in these 1000 completed case data for comparison purposes, using eight different missing data scenarios. Third, missing values were imputed using five different imputation methods (1, random imputation; 2, item mean; 3, individual mean; 4, multiple imputation, and 5, sparse nonnegative matrix factorization. The performance for each imputation method was assessed using the root mean square error and dimension score bias.

Results
The five imputation methods yielded close root mean square errors, with an advantage for the multiple imputation. The bias differences were greater regarding the dimension scores, with a clear advantage for multiple imputation. The worst performance was achieved by the mean imputation methods.

Discussion and Conclusions
We recommend the use of multiple imputation to handle missing data in HSOPS-based surveys, whereas mean imputation methods should be avoided. Overall, these results suggest the possibility of optimizing the HSOPS instrument, which should be reduced without loss of overall information.
An Ancillary Sign in the Diagnosis of Bankart and Bankart Variants in Patients With Glenohumeral Instability on Nonarthrographic Magnetic Resonance Imaging: Preglenoid Fat Thickness
Authors: Polat Gökhan , Oğul Hayri , Yalcin Ahmet , Tuncer Kutsi , Kaya Serhat , Pirimoğlu Berhan , Sade Recep , Kantarci Mecit 
Published: March 2019

Objective
We investigated whether preglenoid fat thickness can be used as an ancillary sign for preliminary diagnosis of Bankart and its variants on nonarthrographic magnetic resonance imaging.

Materials and Methods
Sixty-eight anterior dislocation patients were included in this retrospective study. Forty-eight patients with Bankart and its variants, as detected by arthroscopy and magnetic resonance arthrography, were included in the study group. The study group was divided into osseous Bankart, labral Bankart, Perthes, and anterior labroligamentous periosteal sleeve avulsion subgroups. Twenty patients with no detected labral pathologies by arthroscopy and magnetic resonance arthrography were included in the control group. In these patients, preglenoid fat tissue thickness at the anteroinferior portion was measured at the level of the glenoid tubercle. Thickness of preglenoid fat tissue was compared between groups with different labral pathologies and those with normal labrum.

Results
The preglenoid fat tissue thickness was 2.65 ± 1.05 mm in the control group and 0.90 ± 0.59 mm in the study group (P < 0.0001). Patients with preglenoid fat tissue thinner than 1.6 mm showed a significantly increased likelihood of having Bankart and its variants in receiver operating characteristic analysis (sensitivity of 95.8% and specificity of 85%). There was no significant difference in preglenoid fat tissue thickness between the subgroups of the study group (osseous Bankart, labral Bankart, Perthes, and anterior labroligamentous periosteal sleeve avulsion).

Conclusion
Preglenoid fat tissue measurement can be used as an ancillary sign in the diagnosis of Bankart and Bankart variants in glenohumeral instability patients.
Identification of Nonaggressive Pulmonary Nodules Using an Optimized Scoring System
Authors: Stephens Matthew J. , Rho Byung H. , Curran-Everett Douglas , Carr Laurie , Bowler Russell , Lynch David 
Published: March 2019

Purpose:
The purpose of this study was to define the optimal scoring method for identifying benign intrapulmonary lymph nodes.

Materials and Methods:
Subjects for this study were selected from the COPDGene study, a large multicenter longitudinal observational cohort study. A retrospective case-control analysis was performed using identified nodules on a subset of 377 patients who demonstrated 765 pulmonary nodules on their baseline computed tomography (CT) study. Nodule characteristics of 636 benign nodules (which resolved or showed <20% growth rate at 5 y follow-up) were compared with 51 nodules that occurred in the same lobe as a reported malignancy. Two radiologists scored each pulmonary nodule on the basis of intrapulmonary lymph node characteristics. A simple scoring strategy weighing all characteristics equally was compared with an optimized scoring strategy that weighed characteristics on the basis of their relative importance in identifying benign pulmonary nodules.

Results:
A total of 479 of 636 benign pulmonary nodules had the majority of lymph node characteristics, whereas only 1 subpleural nodule with the majority of lymph node characteristics appeared to be malignant. Only 279 of 479 (58%) of benign pulmonary nodules with the majority of lymph node characteristics were intrafissural or subpleural. The optimized scoring strategy showed improved performance compared with the simple scoring strategy with average area under the curve of 0.80 versus 0.55. Optimized cutoff scores showed negative likelihood values for both readers of <0.2. A simulation showed a potential reduction in CT utilization of up to 36% for Fleischner criteria and up to 5% for LUNG-RADS.

Conclusions:
Nodules with the majority of lymph node characteristics, regardless of location, are likely benign, and weighing certain lymph node characteristics greater than others can improve overall performance. Given the potential to reduce CT utilization, lymph node characteristics should be considered when recommending appropriate follow-up.
Is There a Change in Electroconvulsive Therapy Practice Following the New Mental Health Act 2014 in Victoria?
Authors: Das Partha , Jagadheesan Karuppiah , Walker Frances , Lakra Vinay , Lautenschlager Nicola T. , Ferraro Angelo , Rudolph Devapriya 
Published: March 2019

Objectives
The main objective of our study was to examine whether there has been any change to electroconvulsive therapy (ECT) practice since the new Mental Health Act 2014 (MHA) in a public metropolitan mental health service in Victoria.

Methods
This retrospective study examined any change in ECT rate following the new MHA. We compared sociodemographic, clinical, and ECT-related variables for patients treated before (July 1, 2013, and June 30, 2014) and after (1st July 2014 and the 30th June 2015) the new MHA.

Results
A reduction of 11.15% in ECT use per 1000 admissions and 16.4% in ECT use per 100,000 persons was observed subsequent to the new MHA. Hospital legal status at admission positively predicted the chance of starting ECT treatment under involuntary consent. Hospital legal status at admission and discharge, history of involuntary ECT, and final Clinical Global Impression–Severity scores positively predicted, but the year of treatment negatively predicted the chance of completing ECT treatment under involuntary consent.

Conclusions
The new MHA appeared to have been associated with reduced ECT use and lower rate of completing ECT under involuntary consent.
Simultaneous tracking of division and differentiation from individual hematopoietic stem and progenitor cells reveals within-family homogeneity despite population heterogeneity
Authors: Tak Tamar , Prevedello Giulio , Simon Gaël , Paillon Noémie , Duffy Ken R. , Perié Leïla 
Published: March 2019

AbstractThe advent of high throughput single cell methods such as scRNA-seq has uncovered substantial heterogeneity in the pool of hematopoietic stem and progenitor cells (HSPCs). A significant issue is how to reconcile those findings with the standard model of hematopoietic development, and a fundamental question is how much instruction is inherited by offspring from their ancestors. To address this, we further developed a high-throughput method that enables simultaneously determination of common ancestor, generation, and differentiation status of a large collection of single cells. Data from it revealed that while there is substantial population-level heterogeneity, cells that derived from a common ancestor were highly concordant in their division progression and share similar differentiation outcomes, revealing significant familial effects on both division and differentiation. Although each family diversifies to some extent, the overall collection of cell types observed in a population is largely composed of homogeneous families from heterogeneous ancestors. Heterogeneity between families could be explained, in part, by differences in ancestral expression of cell-surface markers that are used for phenotypic HSPC identification: CD48, SCA-1, c-kit and Flt3. These data call for a revision of the fundamental model of haematopoiesis from a single tree to an ensemble of trees from distinct ancestors where common ancestor effect must be considered. As HSPCs are cultured in the clinic before bone marrow transplantation, our results suggest that the broad range of engraftment and proliferation capacities of HSPCs could be consequences of the heterogeneity in their engrafted families, and altered culture conditions might reduce heterogeneity between families, possibly improving transplantation outcomes.
Beyond early development: observing zebrafish over 6 weeks with hybrid optical and optoacoustic imaging
Authors: Vetschera Paul , Koberstein-Schwarz Benno , Schmitt-Manderbach Tobias , Dietrich Christian , Hellmich Wibke , Chekkoury Andrei , Symvoulidis Panagiotis , Reber Josefine , Westmeyer Gil , López-Schier Hernán , Omar Murad , Ntziachristos Vasilis 
Published: March 2019

AbstractZebrafish animal models have traditionally been used in developmental biology studies but have recently become promising models of cancer, tissue regeneration and metabolic disorders, as well as efficient platforms for functional genomics and phenotype-based drug discovery. Most studies of zebrafish have examined only the embryonic or larval stages of development, yet many questions in developmental biology and biomedicine require analysis of adults, when zebrafish are large and opaque. Conventional microscopy methods are highly sensitive to light scattering and therefore cannot be applied to zebrafish older than a few weeks. We describe a novel multi-modality system that can observe zebrafish from the larval stage to adulthood. Using a hybrid platform for concurrent selective plane illumination microscopy (SPIM) and optoacoustic mesoscopy we show continuous imaging of fish growth over 47 days of development at a similar object size-to-resolution ratio. Using multiple wavelength illumination over the visible and short-wavelength infrared regions, we reveal that the optoacoustic method can follow GFP-based contrast used in SPIM, enabling molecular imaging interrogation in adult fish. Moreover we optoacoustically reveal many other features of zebrafish based on optical contrast not present in SPIM, including contrast from endogenous blood, water and lipids. The hybrid method presented can extend optical imaging to adult zebrafish employed as model systems for studying long-term processes in development, cancer, diabetes and other disorders.