bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cellular Composition and Proliferation Levels in the Hematopoietic Tissue of Black Scorpionfish (Scorpaena porcus L.) Head Kidney and Spleen During the Spawning and Wintering Periods
Authors: Andreyeva Aleksandra Y. , Kukhareva Tatiana A. , Soldatov Alexander A. 
Published: November 2018

ABSTRACTTo identify cells and analyze proliferative activity of hematopoietic tissue, black scorpionfish head kidney and spleen cells were characterized by light microscopy and flow cytometry. Hematopoiesis of black scorpionfish head kidney was formed by the following series: erythropoietic, granulopoietic, lymphopoietic, and thrombopoietic. Flow cytometric analysis allowed dividing blood cells in hematopoietic organs into subpopulations differing by size, granularity, and proliferative activity. Three distinct subpopulations were observed during the wintering period. The number of small low‐granulated cells, identified as lymphocytes and thrombocytes, was 41% ± 4% in both wintering and spawning fish. Proliferating subpopulation of blast (high‐granulated) cells amounted to about 36% of the total cell count with 50% ± 5% of proliferating cells; the largest low‐granulated cells (10% of total cells) comprised maturing white blood cells, monocytes, and macrophages. The spawning period was accompanied with an increase of maturing neutrophils and enhancement of blast cell proliferation. In the spleen three distinct subpopulations were observed. The subpopulation of small low‐granulated cells comprised lymphocytes and thrombocytes similar to the head kidney and amounted 33% ± 4%. Other cells with larger diameter were identified as red blood cells. No proliferation was observed during the wintering period in the spleen, however, spawning induced cell proliferation of erythroblasts (small granulated cells) with the number of dividing cells 84% ± 1%. Anat Rec, 302:1136–1143, 2019. © 2018 Wiley Periodicals, Inc.
Sensitivity and Specificity of Autoantibodies Against CD74 in Nonradiographic Axial Spondyloarthritis
Authors: Riechers Elke , Baerlecken Niklas , Baraliakos Xenofon , Achilles‐Mehr Bakhsh Katrin , Aries Peer , Bannert Bettina , Becker Klaus , Brandt‐Jürgens Jan , Braun Jürgen , Ehrenstein Boris , Euler Hans‐Hartwig , Fleck Martin , Hein Reinhard , Karberg Kirsten , Köhler Lars , Matthias Torsten , Max Regina , Melzer Adelheid , Meyer‐Olson Dirk , Rech Jürgen , Rockwitz Karin , Rudwaleit Martin , Schmidt Reinhold E. , Schweikhard Eva , Sieper Joachim , Stille Carsten , von Hinüber Ulrich , Wagener Peter , Weidemann Heike‐Franziska , Zinke Silke , Witte Torsten 
Published: November 2018

ObjectiveAutoantibodies against CD74 (anti‐CD74) are associated with ankylosing spondylitis (AS). The present multicenter study, the International Spondyloarthritis Autoantibody (InterSpA) trial, was undertaken to compare the sensitivity and specificity of anti‐CD74 and HLA–B27 in identifying patients with nonradiographic axial spondyloarthritis (axSpA).MethodsPatients ages 18–45 years with inflammatory back pain of ≤2 years’ duration and a clinical suspicion of axSpA were recruited. HLA–B27 genotyping and magnetic resonance imaging of sacroiliac joints were performed in all patients. One hundred forty‐nine patients with chronic inflammatory back pain (IBP) not caused by axSpA served as controls, and additional controls included 50 AS patients and 100 blood donors whose specimens were analyzed.ResultsOne hundred patients with inflammatory back pain received a diagnosis of nonradiographic axSpA from the investigators and fulfilled the Assessment of SpondyloArthritis international Society (ASAS) criteria. The mean age was 29 years, and the mean symptom duration was 12.5 months. The sensitivity of IgA anti‐CD74 and IgG anti‐CD74 for identifying the 100 axSpA patients was 47% and 17%, respectively. The specificity of both IgA anti‐CD74 and IgG anti‐CD74 was 95.3%. The sensitivity of HLA–B27 was 81%. The positive likelihood ratios were 10.0 (IgA anti‐CD74), 3.6 (IgG anti‐CD74), and 8.1 (HLA–B27). Assuming a 5% pretest probability of axSpA in chronic back pain patients, the posttest probability, after consideration of the respective positive test results, was 33.3% for IgA anti‐CD74, 15.3% for IgG anti‐CD74, and 28.8% for HLA–B27. A combination of IgA anti‐CD74 and HLA–B27 results in a posttest probability of 80.2%.ConclusionIgA anti‐CD74 may be a useful tool for identifying axSpA. The diagnostic value of the test in daily practice requires further confirmation.
Metallochaperone function of the self‐subunit swapping chaperone involved in the maturation of subunit‐fused cobalt‐type nitrile hydratase
Authors: Xia Yuanyuan , Peplowski Lukasz , Cheng Zhongyi , Wang Tianyi , Liu Zhongmei , Cui Wenjing , Kobayashi Michihiko , Zhou Zhemin 
Published: November 2018

AbstractThe transition metal (iron or cobalt) is a mandatory part that constitutes the catalytic center of nitrile hydratase (NHase). The incorporation of the cobalt ion into cobalt‐containing NHase (Co‐NHase) was reported to depend on self‐subunit swapping and the activator of the Co‐NHase acts as a self‐subunit swapping chaperone for subunit exchange. Here we discovered that the activator acting as a metallochaperone transferred the cobalt ion into subunit‐fused Co‐NHase. We successfully isolated two activators, P14K and NhlE, which were the activators of NHases from Pseudomonas putida NRRL‐18668 and the activator of low‐molecular‐mass NHase from
Rhodococcus rhodochrous J1, respectively. Cobalt content determination demonstrated that NhlE and P14K were two cobalt‐containing proteins. Substitution of the amino acids involved in the C‐terminus of the activators affected the activity of the two NHases, indicating that the potential cobalt‐binding sites might be located at the flexible C‐terminal region. The cobalt‐free NHases could be activated by either of the two activators, and both the two activators activated their cognate NHase more efficiently than did the noncognate ones. This study provided insights into the maturation of subunit‐fused NHases and confirmed the metallochaperone function of the self‐subunit swapping chaperone.
Factors associated with long‐term mortality for stroke unit patients in Latvia
Authors: Mihejeva Illa , Vētra Anita , Bērziņa Guna 
Published: November 2018

AbstractAimThe aim of this study was to evaluate how pre‐stroke risk factors, neurological symptoms, and the level of disability shortly after stroke are associated with poststroke mortality during a 7‐year period after stroke, for persons treated in a stroke unit.MethodsThe data of 231 patients were included in the study. Patients who were treated in the stroke unit at the Riga East University Hospital between February 1, 2009, and July 20, 2009, were included in this study. Three stepwise Cox proportional hazard analyses were performed to analyze mortality in the 7 years following stroke. Pre‐stroke risk factors (type of stroke, arterial hypertension, diabetes mellitus, atrial fibrillation, smoking, alcohol abuse, obesity, recurrent stroke, age, gender), neurological symptoms (motor deficit, sensory disturbance, aphasia, poststroke urinary incontinence (PSUI), mental status), and limitations of activity (feeding, bathing, grooming, dressing, toilet use, transfers, mobility, stairs) were evaluated as factors associated with mortality after stroke.ResultsA total of 145 (62.8%) patients died during the study period. The final model for each group of factors included only one of the factors used for the analysis. Patients who had alcohol abuse were 40% more likely to die earlier. The hazard for those with PSUI is 1.72 times higher than those without PSUI. The independence in grooming showed a 39% lower likelihood of dying earlier.ConclusionAlcohol abuse as a pre‐stroke risk factor, poststroke urinary incontinence as a neurological symptom, and dependence in grooming as a factor of disability were associated with earlier mortality in the first seven years after stroke.
Determinants of angiographic thrombus burden and impact of thrombus aspiration on outcome in young patients with ST‐segment elevation myocardial infarction
Authors: Ge Junhua , Li Jian , Dong Bingzi , Ning Xianfeng , Hou Bo 
Published: November 2018

AbstractObjectivesWe aimed to investigate the determinants of thrombus burden (TB) and the impact of thrombus aspiration (TA) on outcome in young adults with ST segment elevation myocardial infarction (STEMI).BackgroundThe determinants of TB in young STEMI patients are not fully understood now.MethodsThe 182 young (age ≤ 45 years) STEMI patients, who underwent coronary angiography and percutaneous coronary intervention (PCI) in our hospital from January 2013 to September 2016, were included. Angiographic TB and impact of TA on major adverse cardiac events (MACEs) were evaluated. Median clinical follow‐up period was 875 (641–1,052) days.ResultsAll patients were male, mean age was 40 ± 5 years. High thrombus burden (HTB) was evidenced in 100 (54.9%) patients. TA was performed in 62 out 100 (62%) patients with high TB (HTB) during PCI. The prevalence of hypertension was significantly higher in the HTB group than in the low thrombus burden (LTB) group (75 vs. 17%, P < 0.001). The proportion of smoking, alcohol consumption, and family history of premature coronary artery disease were similar between HTB and LTB groups. During follow‐up, 2 patients died and 31 patients underwent repeat PCI. MACE rate was significantly higher in the HTB group than in the LTB group (24.0 vs. 9.8%, P = 0.012) and significantly lower in HTB patients with TA than HTB patients without TA (14.5 vs. 39.5%, P = 0.018).ConclusionsHypertension is an independent determinant of HTB and TA could be considered as an effective therapeutic option in young male STEMI patients with HTB.
Spontaneous coronary artery dissection treated with magnesium‐made bioresorbable scaffold: 1‐Year angiographic and optical coherence tomography follow‐up
Authors: Quadri Giorgio , Cerrato Enrico , Rolfo Cristina , Varbella Ferdinando 
Published: November 2018

AbstractA young woman admitted with a diagnosis of NSTE‐ACS underwent coronary angiography which showed LAD spontaneous coronary artery dissection treated with magnesium‐made bioresorbable scaffold. We report 1‐year angiographic and OCT findings.
The Effect of pH and Biogenic Ligands on the Weathering of Chrysotile Asbestos: The Pivotal Role of Tetrahedral Fe in Dissolution Kinetics and Radical Formation
Authors: Walter Martin , Schenkeveld Walter D. C. , Reissner Michael , Gille Lars , Kraemer Stephan M. 
Published: November 2018

AbstractChrysotile asbestos is a soil pollutant in many countries. It is a carcinogenic mineral, partly due to its surface chemistry. In chrysotile, FeII and FeIII substitute Mg octahedra (Fe[6]), and FeIII substitutes Si tetrahedra (Fe[4]). Fe on fiber surfaces can generate hydroxyl radicals (HO.) in Fenton reactions, which damage biomolecules. To better understand chrysotile weathering in soils, net Mg and Si dissolution rates over the pH range 3.0–11.5 were determined in the presence and absence of biogenic ligands. Also, HO. generation and Fe bulk speciation of pristine and weathered fibers were examined by EPR and Mössbauer spectroscopy. Dissolution rates were increased by ligands and inversely related to pH with complete inhibition at cement pH (11.5). Surface‐exposed Mg layers readily dissolved at low pH, but only after days at neutral pH. On longer timescales, the slow dissolution of Si layers became rate‐determining. In the absence of ligands, Fe[6] precipitated as Fenton‐inactive Fe phases, whereas Fe[4] (7 % of bulk Fe) remained redox‐active throughout two‐week experiments and at pH 7.5 generated 50±10 % of the HO. yield of Fe[6] at pristine fiber surfaces. Ligand‐promoted dissolution of Fe[4] (and potentially Al[4]) labilized exposed Si layers. This increased Si and Mg dissolution rates and lowered HO. generation to near‐background level. It is concluded that Fe[4] surface species control long‐term HO. generation and dissolution rates of chrysotile at natural soil pH.
Phenanthroline—A Versatile Ligand for Advanced Functional Polymeric Materials
Authors: Rothfuss Hannah , Knöfel Nicolai D. , Tzvetkova Pavleta , Michenfelder Nadine C. , Baraban Sergej , Unterreiner Andreas‐Neil , Roesky Peter W. , Barner‐Kowollik Christopher 
Published: November 2018

AbstractInvited for the cover of this issue are the groups of Andreas‐Neil Unterreiner and Peter W. Roesky at the Karlsruhe Institute of Technology (KIT) and Christopher Barner‐Kowollik at the Queensland University of Technology (QUT). The image depicts the incorporation of phenanthroline ligands for fabricating metal‐ion‐containing functional polymeric materials. Read the full text of the article at 10.1002/chem.201803692.
Flow Cytometry Method Validation Protocols
Authors: Selliah Nithianandan , Eck Steven , Green Cherie , Oldaker Teri , Stewart Jennifer , Vitaliti Alessandra , Litwin Virginia 
Published: November 2018

AbstractAnalytical method validation provides a means to ensure that data are credible and reproducible. This unit will provide a brief introduction to analytical method validation as applied to cellular analysis by flow cytometry. In addition, the unit will provide practical procedures for three different types of validation. The first is a limited validation protocol that is applicable for research settings and non‐regulated laboratories. The second is validation protocol that presents the minimum validation requirements in regulated laboratories. The third is a transfer validation protocol to be used when methods are transferred between laboratories. The recommendations presented in this unit are consistent with the white papers published by the American Association of Pharmaceutical Scientists and the International Clinical Cytometry Society, as well as with Clinical Laboratory Standards Institute Guideline H62: Validation of Assays Performed by Flow Cytometry (currently in preparation). © 2018 by John Wiley & Sons, Inc.
Improve cloud manufacturing supply chain note‐enterprises optimize combination of the Cuckoo search
Authors: Liang Hejun , Sun Lily 
Published: November 2018

SummaryTo realize full sharing of mass enterprises in cloud manufacturing environment and free and fair cooperation among the supply chain note‐enterprises, this paper presents a method of combining multi‐Objective Cuckoo Search (MOCS) with Pareto to make optimization of task completion time and cost on supply chain in order to prove the viability and effectiveness of the method, doing the muti‐objective optimization model simulation experiment by MATLAB. The experimental results show that the algorithm performs better than present commonly used multi‐objective optimization genetic algorithm, particle swarm optimization, and artificial fish school algorithm, have better search capability and larger search range, escape from local optimal solution, and achieve the global minimum; the multi‐Objective Cuckoo Search can optimize the combination of supply chain enterprises on cloud manufacturing platform effectively.
Cobalt‐Catalyzed Reductive Alkylation of Amines with Carboxylic Acids
Authors: Emayavaramban Balakumar , Chakraborty Priyanka , Sundararaju Basker 
Published: November 2018

AbstractDirect reductive alkylation of amines with carboxylic acid is carried out by using an inexpensive, air‐stable cobalt/triphos catalytic system with molecular hydrogen as the reductant. This efficient synthetic method proceeds through reduction and condensation, followed by reduction of the in situ‐generated imine into the amine in a green catalytic process.
Comparison of urinary cytology and fluorescence in situ hybridization in the detection of urothelial neoplasia: An analysis of discordant results
Authors: McHale Teresa , Ohori N. Paul , Cieply Kathy M. , Sherer Carol , Bastacky Sheldon I. 
Published: November 2018

BackgroundWe examine the performance of cytology and FISH in the detection of urothelial carcinoma (UC), and explore the reasons for discrepant results, and potential clinical implications.MethodsUrine samples from 89 patients were prospectively collected for simultaneous cytology and UroVysion FISH, and results correlated with concurrent biopsies and/or clinical or histologic follow‐up data. Corresponding tissue biopsies, where available, were also evaluated by FISH.ResultsSensitivity and specificity of cytology and FISH for the detection of UC was 54.8% and 92% and 50% and 88%, respectively. Only one of seven false‐positive urinary FISH results proved to be an “anticipatory positive” on extended follow‐up. Five of eight (62.5%) high grade (HG) carcinomas with false‐negative urinary FISH, were negative due to the absence/paucity of FISH‐detectable changes in the tumor cells. In atypical cytology cases, the FISH result did not assist in identifying UC. There was no significant difference between an atypical cytology result and a positive FISH result, with respect to the identification of patients with UC.ConclusionsWe found urinary cytology to be more sensitivity and specific than FISH in the detection of UC, though the difference was not statistically significant. Up to 24% of HG UCs are FISH negative due to an absence of FISH‐detectable abnormalities in the tumor cells. Paucity of neoplastic cells in the urine also contributes to false‐negative FISH results in both HG and low grade tumors. Negative urinary FISH cannot be taken alone as indicating the absence of significant disease in patients with atypical cytology.
Effect of temperature on the biological parameters of the cabbage aphid Brevicoryne brassicae
Authors: Soh Bernard Steve Baleba , Kekeunou Sevilor , Nanga Nanga Samuel , Dongmo Michel , Rachid Hannah 
Published: November 2018

AbstractThe cabbage aphid, Brevicoryne brassicae, is a pest of many plants of the Brassicaceae family including cabbage, Brassica oleracea Linnaeus, 1753. We investigated the effect of temperature on the biological parameters of B. brassicae using different temperature‐based models incorporated in the Insect Life Cycle Modelling (ILCYM) software. Nymphs of first stage were individually placed in the incubators successively set at 10°C, 15°C, 20°C, 25°C, 30°C, and 35°C; 75 ± 5% RH; and L12: D12‐hr photoperiods. We found that first nymph reached the adult stage after 18.45 ± 0.04 days (10°C), 10.37 ± 0.26 days (15°C), 6.42 ± 0.07 days (20°C), 5.076 ± 0.09 days (25°C), and 5.05 ± 0.10 days (30°C), and failed at 35°C. The lower lethal temperatures for B. brassicae were 1.64°C, 1.57°C, 1.56°C, and 1.62°C with a thermal constant for development of 0.88, 0.87, and 0.08, 0.79 degree/day for nymphs I, II, III, and IV, respectively. The temperatures 10, 30, and 35°C were more lethal than 15, 20, and 25°C. Longevity was highest at 10°C (35.07 ± 1.38 days). Fertility was nil at 30°C and highest at 20°C (46.36 ± 1.73 nymphs/female). The stochastic simulation of the models obtained from the precedent biological parameters revealed that the life table parameters of B. brassicae were affected by the temperature. The net reproduction rate was highest at 20°C and lowest at 30°C. The average generation time decreased from 36.85 ± 1.5 days (15°C) to 6.86 ± 0.1 days (30°C); the intrinsic rate of increase and the finite rate of increase were highest at 25°C. In general, the life cycle data and mathematical functions obtained in this study clearly illustrate the effect of temperature on the biology of B. brassicae. This knowledge will contribute to predicting the changes that may occur in a population of B. Brassiace in response to temperature variation.
Effects of removing woody cover on long‐term population dynamics of a rare annual plant (Agalinis auriculata): A study comparing remnant prairie and oldfield habitats
Authors: Alexander Helen M. , Collins Cathy D. , Reed Aaron W. , Kettle W. Dean , Collis Daniel A. , Christiana Lucy D. , Salisbury Vaughn B. 
Published: November 2018

AbstractWorldwide, grasslands are becoming shrublands/forests. In North America, eastern red cedar (Juniperus virginiana) often colonizes prairies. Habitat management can focus on woody removal, but we often lack long‐term data on whether removal leads to population recovery of herbaceous plants without seeding. We undertook a long‐term study (17 years) of numbers of the rare annual plant Agalinis auriculata in a gridwork of 100 m2 plots in adjacent prairie and oldfield sites in Kansas, USA. We collected data before and after removal of Juniperus virginiana at the prairie. Plant population sizes were highly variable at both sites and over time. High numbers of plants in a plot 1 year were often followed by low numbers the following year, suggesting negative density‐dependence. Plant numbers were lowest with extensive woody cover and with low precipitation. After woody plant removal, A. auriculata increased dramatically in abundance and occupancy in most years; increases were also seen at the oldfield, suggesting later survey years were overall more favorable. Synthesis and applications: Removal of woody plants led to increased numbers of a rare annual prairie plant, without seeding. Multiple years of data were essential for interpretation given extreme temporal variability in numbers. The largest prairie population was 7 years following tree removal, showing that positive effects of management can last this long. This species also fared well in oldfield habitat, suggesting restoration opportunities. Given that land managers are busy, time‐efficient field methods and data analysis approaches such as ours offer advantages. In addition to general linear models, we suggest Rank Occupancy‐Abundance Profiles (ROAPs), a simple‐to‐use data visualization and analysis method. Creation of ROAPs for sites before and after habitat management helps reveal the degree to which plant populations are responding to management with changes in local density, changes in occupancy, or both.
Thermal tolerance of Mediterranean marine macrophytes: Vulnerability to global warming
Authors: Savva Ioannis , Bennett Scott , Roca Guillem , Jordà Gabriel , Marbà Núria 
Published: November 2018

AbstractThe Mediterranean Sea is warming at three times the rate of the global ocean raising concerns about the vulnerability of marine organisms to climate change. Macrophytes play a key role in coastal ecosystems, therefore predicting how warming will affect these key species is critical to understand the effects of climate change on Mediterranean coastal ecosystems. We measured the physiological performance of six dominant native Mediterranean macrophytes under ten temperature treatments ranging from 12 to 34°C to examine their thermal niche, and vulnerability to projected warming in the western Mediterranean up until 2100. Among the macrophytes tested, Cymodocea nodosa was the species with the highest thermal optima and it was beyond current summer temperature. Therefore, C. nodosa may benefit from projected warming over the coming century. The optimal temperature for growth of the other species (Posidonia oceanica, Cystoseira compressa, Padina pavonica, Caulerpa prolifera, and Halimeda tuna) was lower. Similarly, the species presented different upper lethal limits, spanning at least across 5.1°C between 28.9°C (P. oceanica) and >34°C (C. nodosa). Our results demonstrate the variable physiological responses of species within the same local community to temperature changes and highlight important potential differences in climate change vulnerability, among species within coastal marine ecosystems.