bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systems Genetics of Hepatic Metabolome Reveals Octopamine as a Target for Non-Alcoholic Fatty Liver Disease Treatment
Authors: Brial Francois , Le Lay Aurélie , Hedjazi Lyamine , Tsang Tsz , Fearnside Jane F. , Otto Georg W. , Alzaid Fawaz , Wilder Steven P. , Venteclef Nicolas , Cazier Jean-Baptiste , Nicholson Jeremy K. , Day Chris , Burt Alastair D. , Gut Ivo G. , Lathrop Mark , Dumas Marc-Emmanuel , Gauguier Dominique 
Published: March 2019

AbstractNon-alcoholic fatty liver disease (NAFLD) is often associated with obesity and type 2 diabetes. To disentangle etiological relationships between these conditions and identify genetically-determined metabolites involved in NAFLD processes, we mapped 1H nuclear magnetic resonance (NMR) metabolomic and disease-related phenotypes in a mouse F2 cross derived from strains showing resistance (BALB/c) and increased susceptibility (129S6) to these diseases. Quantitative trait locus (QTL) analysis based on single nucleotide polymorphism (SNP) genotypes identified diet responsive QTLs in F2 mice fed control or high fat diet (HFD). In HFD fed F2 mice we mapped on chromosome 18 a QTL regulating liver micro- and macrovesicular steatosis and inflammation, independently from glucose intolerance and adiposity, which was linked to chromosome 4. Linkage analysis of liver metabolomic profiling data identified a QTL for octopamine, which co-localised with the QTL for liver histopathology in the cross. Functional relationship between these two QTLs was validated in vivo in mice chronically treated with octopamine, which exhibited reduction in liver histopathology and metabolic benefits, underlining its role as a mechanistic biomarker of fatty liver with potential therapeutic applications.
Comparative analysis of toxicity reduction of wastewater in twelve industrial park wastewater treatment plants based on battery of toxicity assays
Authors: Yu Yue , Wu Bing , Jiang Linmiao , Zhang Xu-Xiang , Ren Hong-Qiang , Li Mei 
Published: March 2019

AbstractWastewater treatment plants (WWTPs) in industrial parks provide centralized treatment for industrial and domestic wastewater. However, the information on toxicity reduction of wastewater and its correlation with treatment process in industrial park is limited. This study compared the toxicity reduction of wastewater in 12 industrial park WWTPs based on battery of toxicity assays. Nine toxic endpoints involving microorganism, phytoplankton, zooplankton, plant and human cell lines were applied. All the influents of WWTPs induced high toxicities, which were significantly reduced after the treatments from 7 of the studied WWTPs. However, the effluents of five WWTPs induced higher toxicity in one or more toxic endpoints compared to the influents. This study also found that most of anaerobic-anoxic-oxic (A2/O)-based processes had good removal efficiency of wastewater toxicity, while the sequencing batch reactor (SBR)-based processes had the lowest removal efficiency. Moreover, low correlation coefficients were obtained among all toxic endpoints, indicating that battery of toxicity assays was necessary to completely characterize the toxicity and risk of wastewater in industrial parks. This study shed new lights to the toxicity reduction of wastewater and its correlation with treatment process, which is very useful for the design, management and operation of WWTPs in industrial parks.
Validity of an observational assessment tool for multifaceted evaluation of faecal condition
Authors: Ohno Harumi , Murakami Haruka , Tanisawa Kumpei , Konishi Kana , Miyachi Motohiko 
Published: March 2019

AbstractFaecal volume, form, colour, and odour are associated with various diseases, dietary habits, and the gut microbiome. Multifaceted assessment of faecal condition will be needed for future research and practice. Faecal observation has advantages, as it is non-invasive, frequent, and easy. We have developed and validated an illustrative card tool for comprehensively faecal assessment. In 38 healthy adults, observations of volume, form, colour, and odour of faeces using the tool were compared to the objective characteristics of the actual faeces determined using a weighing scale, moisture meter, hardness meter, colourimeter, and odour measuring device. A significant positive correlation (ρ = 0.778) was observed between the number of faecal model (2 cm × 10 cm) units and the actual weight. The Bristol Stool Form Scale showed a significant positive correlation with the moisture content (ρ = 0.717) and negative correlations with faecal hardness (ρ = −0.843) and adhesiveness (ρ = −0.761). The L*a*b* colour space values of the stool differed significantly among observational judgments using the colour card tool. No significant correlation was observed between the observation of odour and the measured odour index. In conclusion, the faecal volume, form, and colour can be estimated by observation using the multifaceted assessment card tool.
The degradation of levofloxacin in infusions exposed to daylight with an identification of a degradation product with HPLC-MS
Authors: Czyrski Andrzej , Anusiak Katarzyna , Teżyk Artur 
Published: March 2019

AbstractIn this paper the decomposition product of levofloxacin was identified. Levofloxacin was dissolved in 0.9% NaCl, 5% glucose, and Ringer’s solution. The solutions were divided into two batches: the first one was exposed to daylight and the second one was protected from it. The solutions were stored at the room temperature. The qualitative analysis of the degradation product was performed using MS and TOF detectors. The quantitative assay was done by a validated HPLC method. Visual inspection and pH assessment were done. Levofloxacin protected from daylight remained stable in 0.9% NaCl, 5% dextrose, and Ringer’s solution. A slight decomposition of the analyte was observed in the solutions exposed to daylight with the fastest decomposition rate in Ringer’s solution as compared with 0.9% NaCl and 5% dextrose solutions. The degradation product of levofloxacin detected with MS was levofloxacin N-oxide. Levofloxacin solutions should be protected from direct daylight to maintain drug stability. Levofloxacin N-oxide is formed regardless of the solvent used.
The Transmembrane Conformation of the Influenza B Virus M2 Protein in Lipid Bilayers
Authors: Mandala Venkata S. , Liao Shu-Yu , Gelenter Martin D. , Hong Mei 
Published: March 2019

AbstractInfluenza A and B viruses cause seasonal flu epidemics. The M2 protein of influenza B (BM2) is a membrane-embedded tetrameric proton channel that is essential for the viral lifecycle. BM2 is a functional analog of AM2 but shares only 24% sequence identity for the transmembrane (TM) domain. The structure and function of AM2, which is targeted by two antiviral drugs, have been well characterized. In comparison, much less is known about the structure of BM2 and no drug is so far available to inhibit this protein. Here we use solid-state NMR spectroscopy to investigate the conformation of BM2(1–51) in phospholipid bilayers at high pH, which corresponds to the closed state of the channel. Using 2D and 3D correlation NMR experiments, we resolved and assigned the13C and15N chemical shifts of 29 residues of the TM domain, which yielded backbone (φ, ψ) torsion angles. Residues 6–28 form a well-ordered α-helix, whereas residues 1–5 and 29–35 display chemical shifts that are indicative of random coil or β-sheet conformations. The length of the BM2-TM helix resembles that of AM2-TM, despite their markedly different amino acid sequences. In comparison, large15N chemical shift differences are observed between bilayer-bound BM2 and micelle-bound BM2, indicating that the TM helix conformation and the backbone hydrogen bonding in lipid bilayers differ from the micelle-bound conformation. Moreover, HNchemical shifts of micelle-bound BM2 lack the periodic trend expected for coiled coil helices, which disagree with the presence of a coiled coil structure in micelles. These results establish the basis for determining the full three-dimensional structure of the tetrameric BM2 to elucidate its proton-conduction mechanism.
Coherent Response of Vietnam and Sumatra-Java Upwellings to Cross-Equatorial Winds
Authors: Wu Chau-Ron , Wang Li-Chiao , Wang You-Lin , Lin Yong-Fu , Chiang Tzu-Ling , Hsin Yi-Chia 
Published: March 2019

AbstractUpwelling off Vietnam in the South China Sea (SCS) and the Sumatra–Java upwelling in the Indian Ocean significantly modulate regional variation in climate. Although located in different hemispheres, these upwellings nearly concur during the boreal summer; both are the result of wind-induced Ekman divergence. Beyond seasonal time scales, the two upwellings were not synchronous in 1998. In the summer of 1998, upwelling off Vietnam was almost absent, generating the warmest summer on record in the SCS. We demonstrated that the El Niño–Southern Oscillation, which was highly correlated with the upwelling in previous studies, was not solely responsible for this variability. Wind trajectory analyses revealed that cross-equatorial winds, which had passed over the Sumatra–Java upwelling site about 2 weeks earlier, were a rapid force acting on SCS summer upwelling. In the summer of 1998, SCS winds were greatly perturbed due to an anomalous wind path that blew toward the SCS through the Sulu Sea. Our findings suggest that not only the resulting weakening but also the perturbation of the SCS winds prevented the formation of summer upwelling off central Vietnam in that year.
Fabrication of nanoribbons by dielectrophoresis assisted cold welding of gold nanoparticles on mica substrate
Authors: Cha Song-Hyun , Kang Se-Hyeon , Lee You Jeong , Kim Jae-Hyun , Ahn Eun-Young , Park Youmie , Cho Seonho 
Published: March 2019

AbstractUsing alternating current electric fields, nanoribbons are fabricated from an aqueous suspension of gold nanoparticles (AuNPs) on mica substrate without resorting to further chemical functionalization of AuNPs. The potential and kinetic energies of AuNPs subjected to attractive forces from a mica substrate provide sufficient energy to pass the diffusion barrier of the gold atoms, which eventually leads to cold welding. A dielectrophoresis force exerted on polarizable particles in a non-uniform electric field contributes to the directed growth of the cold welding that occurs by adjusting the lattice structures of AuNPs. Depending on the concentration of the AuNP suspension, the frequency of the electric field, and the geometry of electrodes, various morphologies of nanoribbons are fabricated. It turns out that the welded region is nearly perfect to provide the same crystal orientation and strength as the rest of the nanostructures, which can be extensively utilized in the fabrication of various nanostructures.
Evaluation of offset of conjunctival hyperemia induced by a Rho-kinase inhibitor; 0.4% Ripasudil ophthalmic solution clinical trial
Authors: Sakamoto Emi , Ishida Waka , Sumi Tamaki , Kishimoto Tatsuma , Tada Kentaro , Fukuda Ken , Yoneda Tsuyoshi , Kuroiwa Hajime , Terao Etsuko , Fujisawa Yasuko , Nakakura Shunsuke , Jian Koji , Okumichi Hideaki , Kiuchi Yoshiaki , Fukushima Atsuki 
Published: March 2019

AbstractGlaucoma leads to irreversible blindness. Numerous anti-glaucoma eye drops have been developed. Unfortunately, many patients with glaucoma still suffer from progressive visual disorders. Recently, ripasudil hydrochloride hydrate, a selective Rho-associated protein kinase inhibitor, was launched for the treatment of glaucoma. However, adverse events, such as conjunctival hyperemia, are often noted in clinical trials using healthy subjects. Therefore, we investigated the onset, offset, and kinetic changes of conjunctival hyperemia induced by ripasudil ophthalmic solution in patients with open-angle glaucoma or ocular hypertension who had already been treated with anti-glaucoma eye drops other than ripasudil. Conjunctival hyperemia was evaluated by both clinical grading by 3 ophthalmic physicians and pixel coverage of conjunctival blood vessels determined by conjunctival hyperemia-analyzing software. Conjunctival hyperemia appeared within 10 min post-instillation in most of the participants. Clinical grade and pixel coverage increased significantly 10 min post-instillation and then decreased. In most of the participants, hyperemia resolved within 2 h. Median conjunctival hyperemia offset was 90 min. A tendency of monotonic increase was observed between clinical grade and pixel coverage. Taken altogether, hyperemia induced by ripasudil was transient in glaucoma patients who had already been treated with anti-glaucoma eye drops other than ripasudil.
Perovskite ferroelectric tuned by thermal strain
Authors: Tyunina M. , Pacherova O. , Peräntie J. , Savinov M. , Jelinek M. , Jantunen H. , Dejneka A. 
Published: March 2019

AbstractModern environmental and sustainability issues as well as the growing demand for applications in the life sciences and medicine put special requirements to the chemical composition of many functional materials. To achieve desired performance within these requirements, innovative approaches are needed. In this work, we experimentally demonstrate that thermal strain can effectively tune the crystal structure and versatile properties of relatively thick films of environmentally friendly, biocompatible, and low-cost perovskite ferroelectric barium titanate. The strain arises during post-deposition cooling due to a mismatch between the thermal expansion coefficients of the films and the substrate materials. The strain-induced in-plane polarization enables excellent performance of bottom-to-top barium titanate capacitors akin to that of exemplary lead-containing relaxor ferroelectrics. Our work shows that controlling thermal strain can help tailor response functions in a straightforward manner.
Description and Verification of the Fundamental Current Mechanisms in Silicon Carbide Schottky Barrier Diodes
Authors: Nicholls Jordan , Dimitrijev Sima , Tanner Philip , Han Jisheng 
Published: March 2019

AbstractAttempts to model the current through Schottky barrier diodes using the two fundamental mechanisms of thermionic emission and tunnelling are adversely impacted by defects and second order effects. This has led to the publication of countless different models to account for these effects, including some with non-physical parameters. Recently, we have developed silicon carbide Schottky barrier diodes that do not suffer from second order effects, such as excessive leakage, carrier generation and recombination, and non-uniform barrier height. In this paper, we derive the foundational current equations to establish clear links between the fundamental current mechanisms and the governing parameters. Comparing these equations with measured current–voltage characteristics, we show that the fundamental equations for tunnelling and thermionic emission can accurately model 4H silicon carbide Schottky barrier diodes over a large temperature and voltage range. Based on the obtained results, we discuss implications and misconceptions regarding barrier inhomogeneity, barrier height measurement, and reverse-bias temperature dependencies.
Adsorption mechanism of Cr(VI) onto GO/PAMAMs composites
Authors: Liu Han , Zhang Fan , Peng Zhiyuan 
Published: March 2019

AbstractGraphene oxide/polyamidoamine dendrimers (GO/PAMAMs) composites were used to remove Cr(VI) from simulated effluents, the adsorption kinetics and thermodynamics of Cr(VI) onto GO/PAMAMs were systematically investigated. The results showed that the optimum pH value was 2.5, the removal percentage reached 90.7% for 30 mg/L of Cr(VI) within 120 min. The adsorption process was well described by pseudo-second-order kinetic model. The maximum adsorption capacities of Cr(VI) onto GO/PAMAMs were found to be 131.58, 183.82 and 211.42 mg/g at 293.15, 303.15 and 313.15 K, respectively, which were calculated from the Langmuir model equation. The adsorption thermodynamic parameters indicate that the adsorption of Cr(VI) onto GO/PAMAMs is a spontaneous endothermic process. The XPS analysis reveals the adsorption and removal mechanism of Cr(VI) on GO/PAMAMs that first the Cr(VI) binds to the protonated amine of GO/PAMAMs, then Cr(VI) be reduced to Cr(III) with the assistance of π-electrons on the carbocyclic six-membered ring of GO in GO/PAMAMs, and then Cr(III) was released into solution under the electrostatic repulsion between the Cr(III) and the protonated amine groups.
B-cell translocation gene 2 enhances fibroblast growth factor 21 production by inducing Kruppel-like factor 15
Authors: Kim Yong Deuk , Hwang Seung-Lark , Jeon Hwang-Ju , Jeon Yong Hyun , Nedumaran Balachandar , Kim Kyeongsoon , Lee Sung-Eun 
Published: March 2019

AbstractFibroblast growth factor 21 (FGF21) is a hormone that is vital for the regulation of metabolic homeostasis. In the present study, we report that Kruppel-like factor 15 (KLF15) is a novel mediator of b-cell translocation gene 2 (BTG2)-induced FGF21 biosynthesis. The expression levels of hepatic Fgf21, Btg2, and Klf15, and the production of serum FGF21 increased significantly in fasted and forskolin (FSK)-treated mice. The overexpression of Btg2 using an adenoviral delivery system elevated FGF21 production by upregulating Klf15 transcription. Interaction studies indicated that BTG2 was co-immunoprecipitated with KLF15 and recruited by the Fgf21 promoter. The disruption of hepatic Btg2 and Klf15 genes markedly attenuated the induction of Fgf21 expression and FGF21 biosynthesis in fasted mice. Similarly, the FSK-mediated induction of Fgf21 promoter activity was strikingly ablated by silencing of Btg2 and Klf15. Taken together, these findings suggest that KLF15 and BTG2 are mediators of fasting-induced hepatic FGF21 expression. Therefore, targeting BTG2 and KLF15 might be a therapeutically important strategy for combat metabolic dysfunction.
Promotion of osteogenic differentiation by non-thermal biocompatible plasma treated chitosan scaffold
Authors: Li Ying , Kim Ji Hye , Choi Eun Ha , Han Ihn 
Published: March 2019

AbstractNon-thermal biocompatible plasma (NBP) has recently emerged as an attractive tool for surface modification of biomaterials in tissue engineering. Three dimensional chitosan scaffolds have been widely used in bone tissue engineering due to biodegradable and biocompatible properties. The present study aimed to evaluate osteogenic potential of NBP treated chitosan scaffold. The surface characteristics of scaffolds were analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD), cell proliferation and differentiation was tested with osteoprogenitor cell line MC3T3-E1. The results show that NBP modified scaffold increase cell metabolic by MTT assay and live/dead assay. More importantly, we evidenced enhancement of osteogenic differentiation on NBP treated scaffolds by an increase of alkaline phosphatase (ALP) activity, high degree of extracellular mineralization and up-regulated osteogenic marker genes expression level. The findings in our study highlighted NBP as the innovative method to modified chitosan scaffold and to fine-tuning the scaffold a more suitable and beneficial biomaterial for in vivo bone tissue engineering and clinical bone defects therapies.
Pyruvate-depleting conditions induce biofilm dispersion and enhance the efficacy of antibiotics in killing biofilms in vitro and in vivo
Authors: Goodwine James , Gil Joel , Doiron Amber , Valdes Jose , Solis Michael , Higa Alex , Davis Stephen , Sauer Karin 
Published: March 2019

AbstractThe formation of biofilms is a developmental process initiated by planktonic cells transitioning to the surface, which comes full circle when cells disperse from the biofilm and transition to the planktonic mode of growth. Considering that pyruvate has been previously demonstrated to be required for the formation of P. aeruginosa biofilms, we asked whether pyruvate likewise contributes to the maintenance of the biofilm structure, with depletion of pyruvate resulting in dispersion. Here, we demonstrate that the enzymatic depletion of pyruvate coincided with the dispersion of established biofilms by S. aureus and laboratory and clinical P. aeruginosa isolates. The dispersion response was dependent on pyruvate fermentation pathway components but independent of proteins previously described to contribute to P. aeruginosa biofilm dispersion. Using porcine second-degree burn wounds infected with P. aeruginosa biofilm cells, we furthermore demonstrated that pyruvate depletion resulted in a reduction of biofilm biomass in vivo. Pyruvate-depleting conditions enhanced the efficacy of tobramycin killing of the resident wound biofilms by up to 5-logs. Our findings strongly suggest the management of pyruvate availability to be a promising strategy to combat biofilm-related infections by two principal pathogens associated with wound and cystic fibrosis lung infections.
Large Liver Blood Vessels and Bile Ducts Are Not Damaged by Electrochemotherapy with Bleomycin in Pigs
Authors: Zmuc Jan , Gasljevic Gorana , Sersa Gregor , Edhemovic Ibrahim , Boc Nina , Seliskar Alenka , Plavec Tanja , Brloznik Maja , Milevoj Nina , Brecelj Erik , Kos Bor , Izlakar Jani , Jarm Tomaz , Snoj Marko , Stukelj Marina , Miklavcic Damijan , Cemazar Maja 
Published: March 2019

AbstractThe first clinical studies on the use of electrochemotherapy to treat liver tumours that were not amenable to surgery or thermal ablation techniques have recently been published. However, there is still a lack of data on the effects of electrochemotherapy on normal liver tissue. Therefore, we designed a translational animal model study to test whether electrochemotherapy with bleomycin causes clinically significant damage to normal liver tissue, with emphasis on large blood vessels and bile ducts. We performed electrochemotherapy with bleomycin or delivered electric pulses alone using a potentially risky treatment strategy in eight pigs. Two and seven days after treatment, livers were explanted, and histological analysis was performed. Blood samples were collected before treatment and again before euthanasia to evaluate blood biomarkers of liver function and systemic inflammatory response. We found no thrombosis or other clinically significant damage to large blood vessels and bile ducts in the liver. No clinical or laboratory findings suggested impaired liver function or systemic inflammatory response. Electrochemotherapy with bleomycin does not cause clinically significant damage to normal liver tissue. Our study provides further evidence that electrochemotherapy with bleomycin is safe for treatment of patients with tumours near large blood vessels in the liver.
Imaging Amyloplasts in the Developing Endosperm of Barley and Rice
Authors: Matsushima Ryo , Hisano Hiroshi 
Published: March 2019

AbstractAmyloplasts are plant-specific organelles responsible for starch biosynthesis and storage. Inside amyloplasts, starch forms insoluble particles, referred to as starch grains (SGs). SG morphology differs between species and SG morphology is particularly diverse in the endosperm of Poaceae plants, such as rice (Oryza sativa) and barley (Hordeum vulgare), which form compound SGs and simple SGs, respectively. SG morphology has been extensively imaged, but the comparative imaging of amyloplast morphology has been limited. In this study, SG-containing amyloplasts in the developing endosperm were visualized using stable transgenic barley and rice lines expressing amyloplast stroma-targeted green fluorescent protein fused to the transit peptide (TP) of granule-bound starch synthase I (TP-GFP). The TP-GFP barley and rice plants had elongated amyloplasts containing multiple SGs, with constrictions between the SGs. In barley, some amyloplasts were connected by narrow protrusions extending from their surfaces. Transgenic rice lines producing amyloplast membrane-localized SUBSTANDARD STARCH GRAIN6 (SSG6)-GFP were used to demonstrate that the developing amyloplasts contained multiple compound SGs. TP-GFP barley can be used to visualize the chloroplasts in leaves and other plastids in pollen and root in addition to the endosperm, therefore it provides as a useful tool to observe diverse plastids.