bluuebunny/crossref_metadata_2025_split huggingface dataset:
Goiter parenchyma, blood vessels and lymphatics contain Staphylococcus epidermidis - saprophyte or pathogen?
Authors: Durowicz Sergiusz , Zaleska Marzanna , Olszewski Waldemar L. , Stelmach Ewa , Piskorska Katarzyna , Swoboda-Kopeć Ewa , Tarnowski Wiesław 
Published: July 2019

AbstractBackgroundGoiter in its various clinical and histopathological forms is accompanied by an inflammatory process requiring intensive therapy. The thyroid gland is an organ specifically exposed to the microbial environment due to its close location to the mouth microbiome. A number of bacterial phenotypes has been detected in the inflamed thyroid gland. A question raises as to whether bacteria have not already been present in the thyroid gland before the clinical symptoms of goiter became evident.AimTo answer the questions: a) do the goiter tissue structures contain bacteria, b) if so, which bacterial phenotypes can be identified, c) what are the genetic similarities of the thyroid and periodontal bacterial strains.Material and methodsStudies were carried out in 60 patients with the non-toxic multinodular goiter in 40 cases, toxic multinodular goiter in 10, single adenoma in 3, Hashimoto’s disease with nodular changes in 4 and recurrent thyroid disease in 3. Tissue fragments harvested during surgery were placed on Columbia blood agar base enriched with 5% defibrinated sheep blood. In this method bacteria present in the tissue slowly proliferate in their in vivo transferred to ex-vivo environment, crawl out and form the on-plate colonies. It enables detection of single bacteria usually difficult in a standard planktonic culture.Resultsa) Coagulase-negative Staphylococci were shown growing on culture plates in above 50% of thyroid parenchyma, veins, arteries and adjacent lymphoid tissue specimens, b) tissue-originating colony-forming bacteria appeared on plates on day 3, but in some as late as after 12-21 days, c) all isolates were sensitive to the basic antibiotics, d) bacterial thyroid and oral DNA tests showed similarities indicating possibility of the oral origin, e) the on-plate time-prolonged cultures showed shrinking of the colonies and upon adding liquid medium formed the small variant colonies.ConclusionsThyroid gland tissues contained in above 50% of specimens the coagulase-negative Staphylococci. Over 88% similarity of the genetic pattern of Staphylococcus epidermidis strain from tooth, oropharyngeal and thyroid tissues, estimated with PCR MP technique, suggested their periodontium origin.
Capsicum cultivated under adverse conditions in Southern Japan produces higher concentrations of antioxidants and pungent components
Authors: Kajiya Katsuko , Yamanouchi Hiroki , Tanaka Yurika , Hayashi Hiroka , Minami Yuji 
Published: July 2019

AbstractGrowing crops in sabulous soils is often challenging due to their limited oligotrophy and weak water retention. However, some plants adapt to these adverse growth conditions, and in some cases, favorable properties are imparted to the fruit. This study investigated the influence of the cultivation environment on Capsicum by assessing the levels and functions of both pungent components and antioxidants when cultivated in sandy soils in Southern Japan; these parameters were then compared to those in traditional tropical-origin Capsicum. In seven varieties of Capsicum, the distribution of capsaicin and dihydrocapsaicin differed between the pericarp and seeds within the placenta. The leaves and fruits of Habanero orange and Tabasco, which are the most suitable for cultivation in sandy soil, were collected during the cultivation period and analyzed in terms of their size, color, and pungent component composition. Pungent components were detected in fruits only, and not in leaf or flower samples. In particular, we found that pungent components were generally present within the seeds and placenta. Antioxidant activity and nitric oxide production within human vascular endothelial cells were also evaluated to compare the differences in their functionality. Satsuma-Habanero orange cultivated under adverse conditions possessed the highest antioxidant activity. Furthermore, Satsuma-Capsicum cultivated under adverse conditions exhibited higher levels of antioxidants than traditional tropical-origin peppers, and induced similar levels of nitric oxide production in the vascular endothelial cells. We concluded that Capsicum cultivated in harsh environments produced beneficial effects such as higher levels of antioxidants and capsaicinoids in seeds and placenta. Moreover, the fruits from these plants could be harvested for a significantly longer period and took longer to spoil than traditional Capsicum; thus, they show merit as a viable commercial crop in Japan.
Homeodomain-interacting protein kinase (Hipk) is required for nervous system and muscle structure and function
Authors: Wang Simon , Sinclair Donald A.R. , Kim Hae-Yoon , Kinsey Stephen D. , Yoo Byoungjoo , Wong Kenneth Kin-Lam , Krieger Charles , Harden Nicholas , Verheyen Esther M. 
Published: July 2019

AbstractHomeodomain-interacting protein kinases (Hipk) have been previously associated with cell proliferation and cancer, however, their effects in the nervous system are less well understood. We have used Drosophila melanogaster to evaluate the effects of altered Hipk expression on the nervous system and muscle. Using genetic manipulation of Hipk expression we demonstrate that knockdown and over-expression of Hipk produces early adult lethality, partially due to the effects on the nervous system and muscle involvement. We find that optimal levels of Hipk are critical for the function of dopaminergic neurons and glial cells in the nervous system, as well as muscle. Furthermore, manipulation of Hipk affects the structure of the larval neuromuscular junction (NMJ) and increases motor neuron axonal branching. Hipk regulates the phosphorylation of the synapse-associated cytoskeletal protein Hts (adducin) and modulates the expression of two important protein kinases, Calcium-calmodulin protein kinase II (CaMKII) and Partitioning-defective 1 (PAR-1), all of which may alter neuromuscular function and influence lethality. Hipk also modifies the distribution of an important nuclear protein, TBPH, the fly orthologue of TAR DNA-binding protein 43 (TDP-43), which may have relevance for understanding motor neuron diseases.
Latent variable modelling and variational inference for scRNA-seq differential expression analysis
Authors: Godinho Joana , Carvalho Alexandra M. , Vinga Susana 
Published: July 2019

AbstractDisease profiling, treatment development, and the identification of new cell populations are some of the most relevant applications relying on differentially expressed genes (DEG) analysis. In this context, three leading technologies emerged; namely, DNA microarrays, bulk RNA sequencing (RNA-seq), and single-cell RNA sequencing (scRNA-seq), the main focus of this work. Although scRNA-seq tends to offer more accurate data, it is still limited by many confounding factors. We introduce two novel approaches to assess DEG: extended Bayesian zero-inflated negative binomial factorization (ext-ZINBayes) and single-cell differential analysis (SIENA). In addition, we benchmark the proposed methods with known DEG analysis tools for single-cell and bulk RNA data, using two real public datasets. One contains house mouse cells of two different types, while the other gathers human peripheral blood mononuclear cells divided into four types. The results show that the two procedures can be very competitive with existing methods (scVI, SCDE, MAST, and DEseq) in identifying relevant putative biomarkers. In terms of scalability and correctness, SIENA stands out from ext-ZINBayes and some of the existing methods. As single-cell datasets become increasingly larger, SIENA may emerge as a powerful tool to discover functional differences between two conditions. Both methods are publicly available (https://github.com/JoanaGodinho/SIENA, https://github.com/JoanaGodinho/ext-ZINBayes).
A new strategy for describing the characteristics of bending line in flexible push bending
Authors: Zhang Zengkun , Wu Jianjun , Shang Qi , Jiang Qiang , Yang Junzhou , Guo Ruichao 
Published: July 2019

Purpose
When manufacturing an arc-shaped tube product using push bending process, the transition zone and outfeed zone will inevitably occur. Transition zone and outfeed zone are caused by the kinematical motion of mobile tools. The existence of transition zone and outfeed zone will lead to a big deviation between the forming product and desired shape. To improve the forming quality of arc-shaped products in push bending, the transition zone and outfeed zone are investigated in this paper.

Design/methodology/approach
A piecewise function is used to describe the bending characteristics along bending line, in which a series of vibration parameters are extracted and considered as control values.

Findings
The new strategy is helpful for finding the relationship between tools motion and curvature distribution and improving the bending lines design procedure in flexible push bending.

Originality/value
The new strategy is helpful for finding the relationship between tools motion and curvature distribution and improving the bending lines design procedure in flexible push bending.
Isogeometric boundary integral formulation for Reissner’s plate problems
Authors: Abdelmoety Ahmed K. , Naga Taha H.A. , Rashed Youssef F. 
Published: July 2019

Purpose
This paper aims to develop a new isogeometric boundary element formulation based on non-uniform rational basis splines (NURBS) curves for solving Reissner’s shear-deformable plates.

Design/methodology/approach
The generalized displacements and tractions along the problem boundary are approximated as NURBS curves having the same rational B-spline basis functions used to describe the geometrical boundary of the problem. The source points positions are determined over the problem boundary by the well-known Greville abscissae definition. The singular integrals are accurately evaluated using the singularity subtraction technique.

Findings
Numerical examples are solved to demonstrate the validity and the accuracy of the developed formulation.

Originality/value
This formulation is considered to preserve the exact geometry of the problem and to reduce or cancel mesh generation time by using NURBS curves employed in computer aided designs as a tool for isogeometric analysis. The present formulation extends such curves to be implemented as a stress analysis tool.
Proposing a conceptual framework for customer identity and access management
Authors: Rasouli Hatef , Valmohammadi Changiz 
Published: July 2019

PurposeCustomer identity and access management (CIAM) is a sub-genre of traditional identity and access management (IAM) that has emerged in the past few years to meet evolving business requirements. CIAM focuses on the connectivity with the customer when accessing any type of systems, on-premises and in the cloud, from registration to track. The purpose of this study is to introduce different dimensions of CIAM toward exploiting them in organizations.Design/methodology/approachBased on a thorough review of the relevant literature and semi-structured interview with six experts in the field of digital IAM the necessary data were gathered. Then through the use of content analysis technique, analytic codes and also categories and sub-categories of the data were generated.FindingsResults indicate that four categories, namely, customer identity management, customer access management and information technology and business management are the most important factors affecting the identification of CIAM dimensions.Originality/valueOrganizations could avail of the proposed conceptual model toward identification and offering customized products and services solutions to their customers.
Parallel two-step finite element algorithm for the stationary incompressible magnetohydrodynamic equations
Authors: Ping Yuan , Su Haiyan , Feng Xinlong 
Published: July 2019

Purpose
The purpose of this paper is to propose a local parallel finite element algorithm based on fully overlapping domain decomposition technique to solve the incompressible magnetohydrodynamic equations.

Design/methodology/approach
The algorithm uses a lower-order element pair to compute an initial approximation by the Oseen-type iteration and uses a higher-order element pair to solve a linear system in each processor.

Findings
Besides, the convergence analysis of local parallel finite element algorithm is given. Finally, numerical experiments are presented to verify the efficiency of the proposed algorithm.

Originality/value
Compared with the numerical solution of the common two-step method, this method is easy to realize and can produce a more accurate solution. And, this approach is executed in parallel, so it saves a lot of computational time.
Headwinds in sports sponsoring
Authors: Schafraad Pytrik , Verhoeven Joost W.M. 
Published: July 2019

Purpose
The purpose of this paper is to investigate the effects of a crisis situation in a sports team on the credibility of their sponsor and how the sponsor’s use of various crises response strategies may repair the damage done to their credibility.

Design/methodology/approach
A scenario experiment was conducted with a 2 (pre-/post-crisis) × 4 (sponsor response strategy: denial/distancing/rebuild/no response) mixed factor design. Respondents (n=191) were recruited from a research panel.

Findings
The results confirmed the existence of a spill-over effect: the sponsor’s credibility dropped as a result of the crisis. More interestingly, the effects of the crisis on sponsor credibility were moderated by the response strategy of the sponsor: the harm that the crisis did to the sponsor credibility was aggravated by a denial strategy, but somewhat weakened by a diminishing strategy. A rebuild response unexpectedly improved the credibility of the sponsor.

Practical implications
While partnerships in sports can be risky, because crises can be contagious, such partners can also help one another to protect their credibility. Therefore, this study advocates an integral approach of crisis communication. Sponsors may improve their credibility when they frame their contribution to the solution to the problems as an authentic effort to do good.

Originality/value
Starting from an issue arena perspective, this contribution shows how crises in sports teams also affect sponsors and how sponsors can contribute to the restoration of the damaged credibility with suitable responses to the crisis situation.
Relationship between work motivation scale and TPACK
Authors: Fernandes Semila , Gupta Pooja , Kumar V.V. Ajith 
Published: July 2019

Purpose
The purpose of this paper is to measure the academic motives of faculty in higher education to understand the pattern of relationships between work motivation scale (WMS) and technological pedagogical content knowledge (TPACK).

Design/methodology/approach
The research adopts regression analysis to help model the data collected from 63 faculty members from India. The empirical study investigated the relationship of intrinsic motivation (IntrM), integrated motivation (InteM), identified motivation (IdenM), introjected motivation (IntrjM), external regulation motivation (ExtR) and amotivation (Amot) with each of technological knowledge (TK), (pedagogical knowledge (PK), content knowledge (CK) and TPACK.

Findings
Optimal functioning among faculty was due to “InteM” followed by “IntrM” and “IdenM” that yielded most positive consequence (mean values>3.9). “ExtR” and “Amot” were the negative outcomes that would be counterproductive and result in employee dejection (Mean values<3.1). The results proved that all TPACK variables were positively related to “IntrM” – a self-determined motivation. Additionally, “TK” and “PK’ were positively related to “IntrjM” – a behavior that is regulated by self-esteem and self-worth; “TPACK” depicted positive relationship with “IntrjM” and negative correlation with “IdenM.”

Research limitations/implications
The study supported the fact that TPACK and WMS when analyzed together can create better engagement models among the faculty for an enriching learner experience interaction. Furthermore, it would help identify specific strategies on teacher development training programs through the integration of technology.

Originality/value
The study is original and seeks to inspect the relationship between TPACK and WMS among faculty of higher education in Indian business schools.
Knowledge sharing in higher education institutes
Authors: Farrukh Muhammad , Sajid Muhammad , Zreen Aneeqa , Khalid Rimsha 
Published: July 2019

Purpose
The purpose of this paper is to investigate the relationship between individual characteristics and knowledge sharing (KS) in higher education institutes (HEIs) of Pakistan.

Design/methodology/approach
The study used a quantitative research methodology. The empirical data consisted of 370 responses from the academic staff of six HEIs of Pakistan.

Findings
The findings revealed a significant impact of dispositional factors on KS. More precisely, extroversion, openness to experience, agreeableness, emotional intelligence and religiosity were positively associated with KS, while neuroticism was found to be negatively associated with KS.

Practical implications
This micro-level model of KS has some potential implications for the decision makers in the context of HEIs. To enhance the KS in HEIs, the decision makers should take the findings of this study into consideration while hiring the academicians in the universities. The decision makers should give priority to the potential candidates who have a higher level of extroversion, openness and agreeableness. Further, while making hiring and other job-related strategies, religiosity and emotional intelligence of the potential candidates should not be ignored.

Originality/value
The paper tested a micro-level model of KS in HEIs and contributed to the body of knowledge by jointly investigating the relationship between religiosity, emotional intelligence, personality traits and KS. To the best of researchers’ knowledge, no study has been conducted, so far, which tested these variables jointly. Thus, the present research filled this knowledge gap.
A new methodology for customer behavior analysis using time series clustering
Authors: Abbasimehr Hossein , Shabani Mostafa 
Published: July 2019

Purpose
The purpose of this paper is to propose a new methodology that handles the issue of the dynamic behavior of customers over time.

Design/methodology/approach
A new methodology is presented based on time series clustering to extract dominant behavioral patterns of customers over time. This methodology is implemented using bank customers’ transactions data which are in the form of time series data. The data include the recency (R), frequency (F) and monetary (M) attributes of businesses that are using the point-of-sale (POS) data of a bank. This data were obtained from the data analysis department of the bank.

Findings
After carrying out an empirical study on the acquired transaction data of 2,531 business customers that are using POS devices of the bank, the dominant trends of behavior are discovered using the proposed methodology. The obtained trends were analyzed from the marketing viewpoint. Based on the analysis of the monetary attribute, customers were divided into four main segments, including high-value growing customers, middle-value growing customers, prone to churn and churners. For each resulted group of customers with a distinctive trend, effective and practical marketing recommendations were devised to improve the bank relationship with that group. The prone-to-churn segment contains most of the customers; therefore, the bank should conduct interesting promotions to retain this segment.

Practical implications
The discovered trends of customer behavior and proposed marketing recommendations can be helpful for banks in devising segment-specific marketing strategies as they illustrate the dynamic behavior of customers over time. The obtained trends are visualized so that they can be easily interpreted and used by banks. This paper contributes to the literature on customer relationship management (CRM) as the proposed methodology can be effectively applied to different businesses to reveal trends in customer behavior.

Originality/value
In the current business condition, customer behavior is changing continually over time and customers are churning due to the reduced switching costs. Therefore, choosing an effective customer segmentation methodology which can consider the dynamic behaviors of customers is essential for every business. This paper proposes a new methodology to capture customer dynamic behavior using time series clustering on time-ordered data. This is an improvement over previous studies, in which static segmentation approaches have often been adopted. To the best of the authors’ knowledge, this is the first study that combines the recency, frequency, and monetary model and time series clustering to reveal trends in customer behavior.
Hybrid additive manufacturing of precision engineered ceramic components
Authors: Hinton Jack , Basu Dejan , Mirgkizoudi Maria , Flynn David , Harris Russell , Kay Robert 
Published: July 2019

Purpose
The purpose of this paper is to develop a hybrid additive/subtractive manufacturing platform for the production of high density ceramic components.

Design/methodology/approach
Fabrication of near-net shape components is achieved using 96 per cent Al3O2 ceramic paste extrusion and a planarizing machining operations. Sacrificial polymer support can be used to aid the creation of overhanging or internal features. Post-processing using a variety of machining operations improves tolerances and fidelity between the component and CAD model while reducing defects.

Findings
This resultant three-dimensional monolithic ceramic components demonstrated post sintering tolerances of ±100 µm, surface roughness’s of ∼1 µm Ra, densities in excess of 99.7 per cent and three-point bending strength of 221 MPa.

Originality/value
This method represents a novel approach for the digital fabrication of ceramic components, which provides improved manufacturing tolerances, part quality and capability over existing additive manufacturing approaches.