bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengaruh Pemberian Ampas Kedelai dan Bungkil Inti Sawit (AKBIS) yang Difermentasi dengan Aspergillus niger terhadap Bakteri Usus Broiler
Authors: Nyak Adam Vhodzan Adzima , Nurliana Nurliana , Samadi Samadi 
Published: March 2019

ABSTRAK. Salah satu upaya dilakukan untuk mengganti penggunaan antibiotik adalah mencari imbuhan pakan alternatif seperti pro-prebiotik, bahan pakan herbal dan berbagai imbuhan pakan lainnya. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ampas kedela dan bungkil inti sawit (AKBIS) yang difermentasi dengan Aspergillus niger (A. niger) terhadap mikroflora saluran pencernaan broiler. Sampel saluran pencernaan ayam diperoleh dari 80 ekor broiler yang dibagi menjadi empat perlakuan. Setiap unit perlakuan terdiri dari empat ekor ayam. Setiap perlakuan terdiri dari 5 ulangan yang diberi pakan perlakuan selama 21 dan 35 hari dengan penambahan empat konsentrasi pakan fermentasi yang berbeda (0%, 2%, 4%, 6% dari total pakan). Saluran pencernaan broiler diperiksa ALT mikroba, E.coli, Salmonella dan BAL. Data dianalisis dengan uji Anava melalui program SPSS for windows versi 17.0 dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa AKBIS yang difermentasi dengan A. niger berpengaruh nyata (P0,05) terhadap jumlah mikroba saluran pencernaan broiler. Konsentrasi dan lama pemberian berpengaruh (P0,05) terhadap jumlah mikroba saluran pencernaan broiler. Pemberian AKBIS fermentasi mampu mempertahankan jumlah mikroba yang menguntungkan seperti E. coli dan mengurangi mikroba patogen yaitu Salmonella, serta mampu meningkatkan jumlah bakteri asam laktat dalam saluran pencernaan broiler. Hasil penelitian disimpulkan bahwa AKBIS fermentasi dapat digunakan sebagai sumber probiotik sehingga mampu meningkatkan kualitas broiler dan aman bagi konsumen.The effect of soybean by products and palm kernel meal fermented with Aspergillus niger on broilers intestinal bacteria ABSTRACT. One of the efforts to replace antibiotics in animal feed is by providing other sources of feed additives such as pre- pro biotics, phytogenics, and other feed additives. The aim of this study was to determine the effect of soybean by products and palm kernel meal that fermented with A. niger on the microflora digestive tract of broilers. Chicken digestive tract samples obtained from 80 species of broiler fed treatment for 21 and 35 days. The tretaments in this experiment were different concentrations of fermented soybean by products and palm kernel meal (0%, 2%, 4%, 6%) in the commercial feed. Digestive tract of broiler examined TPC of microbe, E. coli, Salmonella and lactic acid bacteria (LAB). Data were analyzed by Anova with SPSS for Windows version 17.0 and continued with Duncan test. The results showed that soybean by products and palm kernel meal fermented with A. Niger significantly affected (P 0.05) to the number of microbial digestive tract of broilers. Fermented feed was able to maintain the number of non-pathogenic microflora and reduced the number of pathogenic microflora. In conclusion, fermentation of soybean by products with palm kernel meal was able to improve the broiler quality and safe for consumers.
Penambahan Air Jeruk Sambal (Citrus microcarpa) pada Air Minum terhadap Kadar Lemak Daging Broiler
Authors: Adisti Rizqa , Setiawan Duta , Zakiatulyaqin Zakiatulyaqin , Purnomosidi Marjoko 
Published: March 2019

ABSTRAK. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan air jeruk sambal (Citrus microcarpa) pada air minum terhadap penurunan kadar lemak dan taraf lemak daging broiler. Materi yang digunakan adalah ayam umur 1 hari (DOC) strain CP 707 sebanyak 80 ekor yang ditempatkan dalam kandang berukuran (5,5 × 2,5 × 2 m) yang disekat menjadi 20 unit dengan setiap unit percobaan di isi 4 ekor broiler. Pakan yang digunakan adalah pakan komersial (BR10, BR11 dan BR12). Metode yang digunakan adalah rancangan acak lengkap (RAL), dengan 4 perlakuan dan 5 ulangan dengan taraf perlakuan P0 (0% jeruk sambal per 1 L air ), P1 (0,4% jeruk sambal per 1 L air), P2 (0,8% jeruk sambal per 1 L air) dan P3 (1,2% jeruk sambal per 1 L air). Data yang diperoleh dianalisis menggunakan analisis ragam (ANOVA) pada taraf 5% dilanjutkan dengan uji beda nyata jujur (BNJ). Hasil penelitian menunjukan bahwa perlakuan dapat meningkatkan persentase bobot karkas, penurunan persentase lemak abdominal dan persentase lemak daging broiler. Kesimpulan penambahan air jeruk sambal pada air minum dapat menurunkan lemak daging broiler dengan taraf pemberian terbaik 0,8%.(The effect of native orange water (Citrus microcarpa) in drink water on broiler meat fat)ABSTRAK. This research aim to determine the effect of native orange (Citrus microcarpa) to drink water on percentage meat fat broiler and the best level for degrade meat fat. The used materials were day old chick (DOC) strain CP707 as many as 80 birds, were placed in cages with size of (5,5 ×2,5×2 m) is partitioned into 20 units an each unit in the experiment with the contents of 4 chickens. Commercial feed was used, they were (BR10, BR11 and BR12). Completely randomized design (CRD) was applied, with 4 treatment and 5 replications. The level of treatments were P0 (0% native orange for 1 L water), P1 (0,4 native orange for 1 L water), P2 (0,8% native oramge for 1 L water) and P3 (1,2% native orange for 1 L water). Data were analyzed using analysis of variance at level 5% and followed by honesty significant different (HSD).The result showed that there were increasing percentage of carcass, abdominal fat and meat fat. As a conclusion, increment native orange to drink water can degrade broiler meat fat with the best level at 0,8%.
Hubungan antara Tingkah Laku Seksual dengan Produksi Spermatozoa Sapi Brahman
Authors: Sholikah Nisa'us , Sutomo Aris , Widiasmoro Ndaru Pranowo , Wahjuningsih Sri , Yekti Aulia Puspita Anugra , Kuswati Kuswati , Susilawati Trinil 
Published: March 2019

ABSTRAK. Pejantan untuk program inseminasi buatan adalah pejantan unggul yang diseleksi berdasarkan libido dan produksi spermatozoa. Penelitian bertujuan untuk menganalisa hubungan antara tingkah laku seksual dengan produksi spermatozoa sapi Brahman. Materi penelitian adalah 3 pejantan sapi Brahman yang ditampung menggunakan vagina buatan. Metode penelitian adalah observasional dengan mengamati waktu reaksi dan lama ejakulasi. Observasi laboratorium dengan mengamati volume, konsentrasi, dan motilitas individu untuk menghitung total spermatozoa (TS) dan total spermatozoa motil (TSM). Pengamatan dilakukan sebanyak 10 ulangan. Data dianalisa secara deskriptif serta hubungan antara tingkah laku seksual dan produksi spermatozoa dihitung dengan korelasi Pearson. Hasil penelitian menunjukkan terdapat variasi tingkah laku seksual dan produksi spermatozoa pada sapi Brahman. Individu Cipta mempunyai waktu reaksi dan lama ejakulasi paling singkat serta produksi spermatozoa paling rendah. Hubungan signifikan antara waktu reaksi dengan TS serta TSM pada individu Bizzyard dengan koefisien determinasi (R2) sebesar 14 % dan 20%. Hubungan sangat signifikan antara lama ejakulasi dengan TS serta TSM pada individu Cipta dengan R2 sebesar 46% dan 58%. Kesimpulan penelitian adalah terdapat hubungan antara tingkah laku seksual dengan produksi spermatozoa pada sapi Brahman, dengan koefisien korelasi (r) tertinggi sebesar 0,72 dan R2 sebesar 58% antara lama ejakulasi dan total spermatozoa motil pada individu Cipta. (The relationship between sexual behavior and semen production in Brahman bulls) ABSTRACT. Bulls for artificial insemination must be selected based on libido and sperm production. The study aimed to analyze the relationship between sexual behavior with sperm production on Brahman bulls. The material was 3 Brahman bulls collected using artificial vagina. The method was observational by observing reaction time and ejaculation duration. Laboratory observations by observing volume, concentration, and motility to calculate total sperm (TS) and total motile sperm counts (TMSC). Observations were replicated 10 times. The data were analyzed descriptively, the relationship between behavior and sperm production was examined using Pearson correlation. The results showed that there were variations in sexual behavior and sperm production in each bull. Cipta have the shortest reaction time and ejaculation duration and the lowest sperm production. A significant relationship between reaction time and TS and TMSC in Bizzyard with coefficient of determination (R2) of 14% and 20%. A very significant between ejaculation duration with TS and TMSC in Cipta with R2 of 46% and 58%. In conclusion, there was a relationship between sexual behavior and sperm production in Brahman bulls, with the highest correlation coefficient (r) of 0.72 and R2 of 58% between ejaculation duration and the total motile sperm counts in Cipta.
Pengaruh Keberadaan Corpus Luteum terhadap Kualitas dan Tingkat Maturasi Oosit Domba Lokal Umur Pubertas Awal Secara In Vitro
Authors: Widyastuti Rini , Syamsunarno Mas Rizky Anggun Adipurna , Yusuf Alvin , Ridhlo Muhammad Rosyid , Prastowo Sigit 
Published: March 2019

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh keberadaan corpus luteum (CL) pada ovarium domba umur pubertas awal terhadap kualitas oosit hasil koleksi dan tingkat maturasinya secara in vitro (IVM). Sebanyak 279 oosit digunakan, terbagi pada kelompok tanpa CL (CL-) sebanyak 143 dan dengan CL (CL+) sebanyak 136. Oosit dipilih berdasarkan homogenitas sitoplasma dan dikelompokkan sesuai jumlah lapisan sel kumulus yaitu grade 1 (4 lapis), grade 2 (3–4 lapis) dan grade 3 (0–2 lapis). Media IVM menggunakan Tissue Culture Media 199 ditambah antibiotik, Follicle Stimulating Hormone, dan 10% Fetal Bovine Serum. Maturasi dilakukan pada inkubator 38,5°C, 5% CO2 selama 24 jam. Pasca IVM, tingkat kematangan oosit dievaluasi berdasar kemunculan Polar Body I (PB I). Hasil menunjukkan bahwa keberadaan CL tidak berpengaruh terhadap kualitas oosit yang dikoleksi pada semua grade. Keberadaan CL berpengaruh pada tingkat kematangan oosit pada grade 1 sebesar 48,64% dibandingkan CL- sebesar 47,19% (p0,05). Berdasarkan hasil penelitian disimpulkan bahwa berpengaruh pada tingkat kematangan oosit setelah IVM. Hasil penelitian menggambarkan potensi penggunaan oosit ternak umur pubertas awal untuk digunakan lebih lanjut dalam program produksi embrio secara in vitro. (Effect of the presence of corpus luteum on oocytes quality and in vitro maturation rate of ewes at early puberty) ABSTRACT. This study aims to know effect of presence of corpus luteum (CL) to collected oocytes quality and its maturation rate post in vitro maturation (IVM), on local ewes ovary at early puberty. In total 279 oocytes were collected, 143 without CL (CL-) and 136 with CL present (CL+). Oocytes were selected according to sitoplasma hemogenity, divided into 3 grades according to cumulus cell (CC) layer namely Grade 1, 2 and 3 indicated by 4, 3–4, and 0–2 CC layers, respectively. The IVM media was Tissue Culture Media 199 supplemented with antibiotic, Follicel Stimulating Hormone, and 10% Fetal Bovine Serum following culture at 38.5°C and 5% CO2. Twenty four hours post IVM, oocytes were evaluated on the presence of Polar Body I. Result showed that oocytes quality were not different among group in all grades. The present of CL gives better maturation rate in grade 1 compared to CL- (48.64 vs 47.19%; p0.05). The present finding show that the presence of CL improves oocytes maturation rate post IVM. Moreover, this study shows the potency of using oocytes from ewes ovary at early puberty for further in vitro embryo production program
Giant Cell Arteritis in Ophthalmology Practice: a Case Report
Authors: Chernukha V. A. , Atarschikov D. S. , Khamnagdaeva N. V. , Pozharov I. V. 
Published: March 2019

Introduction. Giant cell (temporal) arteritis refers to a group of chronic and acute systemic vasculitis mainly affecting the extracranial and intracranial arteries of large and medium caliber. Loss of vision due to anterior ischemic optic neuropathy (AION) or occlusion of the central retinal artery is one of the most severe and most common complications of giant cell arteritis. This case report describes a patient with giant cell arteritis, the outcome of it was a total vision loss in the right eye. The patient repeatedly visited the ophthalmologists in the outpatient clinics with complaints of intermittent episodes of vision loss.Purpose. To present methods of diagnosis and treatment, through which the doctor at the initial reception will be able to suspect the disease and start treatment timely.Conclusions. The anamnestic criteria for the GCA diagnosis in the practice of ophthalmologist are: female, age over 50 years, headaches with paresthesia, intermittent lameness of the mandible, short-term episodes of vision loss. The necessary laboratory methods of research include: clinical blood test with determination of erythrocyte sedimentation rate, determination of C-reactive protein level.
Electron Microscopic Retina Changes in Rabbit Eyes with Perfluorocarbon Liquids Intravitreal Tamponade
Authors: Arslanov G. M. , Aznabaev B. M. , Mukhamadeev T. R. , Yanbukhtina Z. R. , Dibaev T. I. , Shakirova G. R. 
Published: March 2019

Purpose. Analysis of electron microscopic changes in rabbit eyes with intravitreal tamponade of perfluorocarbon liquids (PFCL) (perfluoro-1,3-dimethylcyclohexane and perfluorodecalin).Material and Methods. The study was performed on Chinchilla breed rabbits. 25G vitrectomy with PFCL intravitreal tamponade was performed on 12 eyes of 6 rabbits (perfluorodecalin (Bausch+Lomb «Dk-line», USA) and perfluoro-1,3-dimethylcyclohexane (ZAO “Optimedservis”, Russia). Standard three-port vitrectomy technique was used. After removal of the vitreous body 2.5 ml of PFCL were injected in vitreous cavity. Research studies were performed in 5, 14 and 30 days after surgery by electron microscopy. Eyes were enucleated in 20 minutes after animal was killed by air embolization. Intact eyes were used as a control. All samples were prepared in same conditions. The damage of the retina architectonics and the presence of intracellular inclusions were evaluated.Results. Tamponade of the vitreous cavity by both types of PFCL in 5, 14 and 30 day caused following similar electron microscopic changes at date: swelling ganglion layer and dystrophy of inner and outer nuclear layer. Electron microscopic changes in outer nuclear layer appeared at 30 days. The photoreceptor neurons were characterized by single ultrastructural changes. Retina pigment epithelium cells had a typical ultrastructure.Conclusion. Intravitreal perfluoro-1,3-dimethylcyclohexane tamponade caused similar electron microscopic changes as well as perfluorodecalin in the experiment and it was relatively harmless to rabbit retina for up to 14 days. Irreversible changes in the retinal ultrastructure were not observed.
Assessment of the 24-hour profle of blood pressure and arterial stiffness in patients with end-stage renal disease
Authors: Minyukhina I. E. , Praskurnichiy E. A. 
Published: March 2019

Objective. The purpose of our study was to research specifc features the daily changes of the vascular stiffness (VS) in patients with end-stage renal disease (ESRD) and to assess the feasibility of using the 24-hour vascular index Pulse Time Index of Norm (PTIN) (the percentage of the 24-hour period during which the pulse wave velocity (PWVao) does not exceed 10 m/second) in the management of arterial hypertension (HTN) in patients after renal transplantation (RT).Design and methods. We examined 158 people, divided into 4 comparable age groups: those receiving program hemodialysis (PGD), patients after RT, patients with essential HTN and healthy volunteers. All of them underwent 24-hour blood pressure (BP) monitoring with a daily evaluation of VS indices and central BP. At follow-up, 27 patients from the PG group underwent all assessments also 1 week and 6 months after transplantation.Results. Patients with ESRD compared with patients with essential HTN had elevated PWVao, night central BP and decrease PTIN. PTIN changes were the most signifcant. In 27 patients a week after the RT a decrease in the PTIN was found in most cases. After 6 months the mean PTIN in the whole group increased again. Our study demonstrates HTN persistence after kidney transplantation can be predicted. Two PTIN states could be predicted by the cutoff PTIN value that was determined in the study: a state of improvement and a state of decline/unchanged state. PTIN cutoff value at 45 % was characterized by 69 % sensitivity, 76 % specifcity and AUC of 0,65. Therefore, baseline PTIN ≥ 45 % (before RT) is associated with its further growth, and a favourable course of HTN.Conclusions. Patients receiving replacement therapy, compared to patients with essential HTN, showed a marked increase in the daily VS and the night central BP. The daily PTIN is the most accurate predictor of the changes in the VS index, the PTIN values before the RT at the PG stage allow predicting the course of HTN after the RT
Comparison of hemodynamic and infarct-limiting effects of preservation solution based on Krebs–Henseleit buffer and HTK solution in the rat model of heterotopic heart transplantation
Authors: Galagudza M. M. , Minasian S. M. , Dmitriev Yu. V. , Poleshenko Ya. I. , Shubina P. Yu. , Protsak E. S. , Uskov I. S. , Sonin D. L. , Kutenkov A. A. , Vlasov T. D. 
Published: March 2019

Objective. To compare the cardioprotective effcacy of a preservation solution based on Krebs–Henseleit buffer and HTK solution in the model of heterotopic heart transplantation in rat.Design and methods. A study was conducted on 12 Wistar rats. The animals were divided into groups depending on the preservation solution used: 1) Krebs–Henseleit buffer-based solution (n = 7), 2) HTK (n = 5). Each experiment consisted of collecting donor heart, preserving it with an appropriate cardioplegic solution, heterotopic transplantation into a recipient rat followed by explantation and evaluation of left ventricular contractility using the Langendorff model and histochemical assessment of the irreversible myocardial damage. Coronary blood ﬂow in the donor heart was assessed in vivo using ultrasound doppler ﬂowmetry. After 3 hours, the donor heart was explanted and connected to the Langendorff apparatus to assess left ventricular contractility, and the myocardium was subjected to histochemical staining with 1 % triphenyltetrazolium chloride for the assessment of the irreversible myocardial damage.Results. In the group of Krebs–Henseleit buffer-based cardioplegic solution, 7 experiments were performed. Myocardial infarct size was 3,5 ± 1,2%, the coronary ﬂow rate was 4,5 ± 1,3 ml/min, and the developed left ventricular pressure of the donor heart was 70 ± 6,3 mmHg at diastolic left ventricular pressure of 10 mmHg. In the HTK solution group (n = 5), in all of the experiments after the start of blood ﬂow, the transplanted heart did not begin to contract, and all 5 hearts remained in an asystole state. Therefore, after the end of the 3-hour reperfusion period, the assessment of the contractility of the left ventricle using the Langendorff apparatus was not carried out. Coronary ﬂow rate was only 0,4 ± 0,1 ml/min, probably resulting from inadequate cardioprotection with HTK solution. Due to the lack of adequate reperfusion, the accurate assessment of the irreversible myocardial damage was impossible in the HTK solution group.Conclusions. The technique of heterotopic heart transplantation in rats is adequate and informative for the study of ischemia-reperfusion myocardial damage as well as for the study of the effectiveness of cardioplegic and cardiac preservation solutions. Cardioplegic solution based on Krebs– Henseleit buffer demonstrated greater cardioprotective effectiveness in our model compared to HTK solution.
Risk factors for cardiovascular complications in women with hypertensive disorders during pregnancy
Authors: Tarasova O. A. , Chulkov V. S. , Sinitsin S. P. , Vereina N. K. , Chulkov Vl. S. 
Published: March 2019

Objective.We performed a cross-sectional study of risk factors and biomarkers of cardiovascular diseases among young women with hypertensive disorders during pregnancy in comparison with women without hypertension during pregnancy.Design and methods.Altogether 117 women were enrolled, 33 were included in the group of hypertensive disorders during pregnancy, and 44 formed the group without hypertension during pregnancy. The control group consisted of 40 patients. The time interval after delivery was on average 5 to 19 years.Results and conclusions.Women with hypertension during pregnancy developed changes in carbohydrate and lipid metabolism associated with hypertension and abdominal obesity, which are the components of the cluster of the metabolic syndrome. These changes are also accompanied by insulin resistance, hyperinsulinemia, adipokine imbalance, changes in fbrinolysis and more pronounced remodeling of the heart and vessels as target organs in hypertension.
Studi Korelasi antara Motivasi Kerja, Kompetensi Profesional Guru, dan Etos Kerja terhadap Kinerja Guru
Authors: Segantara I Gede Mahendra , Yudana I Made , Sunu I Gusti Ketut Arya 
Published: March 2019

The study was conducted in order to figure out and analyze the correlation coefficient between (1) work motivation and the teachers’ performances at SMP Negeri 2 Bebandem; (2) teachers’ professional competencies and the teachers’ performances at SMP Negeri 2 Bebandem; (3) work morale and the teachers’ performances at SMP Negeri 2 Bebandem; (4) work motivation, teachers’ professional competencies, and work morale toward the teachers’ performances at SMP Negeri 2 Bebandem.The study utilized an ex-post facto design, while the data were collected by using questionnaires ofachieving motivation, teachers’ professional competencies, and work morale, as well as the teachers’ performances. The data were analyzed by multiple regressions and correlation test. The population of the study is 43 teachers of SMP Negeri 2 Bebandem. Based on the findings that the variables of work motivation, teachers’ professional competencies, as well as work morale could become predictors to improve the teachers’ performances.