bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rabeprazole suppressed gastric intestinal metaplasia through activation of GPX4-mediated ferroptosis
Authors: Xie Jing , Liang Xinhua , Xie Fangfang , Huang Canxin , Lin Zijun , Xie Shuping , Yang Fangying , Zheng Fengfeng , Geng Lanlan , Xu Wanfu , Gong Sitang , Xiang Li 
Published: November 2024

BackgroundGastric intestinal metaplasia is a common pathological feature in patients with Helicobacter pylori (H. pylori) infection. Rabeprazole was widely used as the first-line regimen for H. pylori infectious treatment. The objective of this study is to explore the mechanism of rabeprazole in gastric intestinal metaplasia treatment.MethodsReal-time PCR, Western blotting (WB) and ROS analysis were conducted to confirm that rabeprazole could induce ferroptosis to suppress gastric intestinal metaplasia. Cellular fraction, luciferase and chromatin immunoprecipitation (ChIP) were used to identify the mechanism underlying rabeprazole modulated ferroptosis.ResultsHerein, we found rabeprazole treatment led to inhibit CDX2 and MUC2 expression, alleviating gastric intestinal metaplasia, which was attributed to enhanced ferroptosis characterized by decreased GPX4 expression. Inhibition of ferroptosis by ferrostatin-1 (Fer-1) could reverse decreased CDX2 and MUC2 expression caused by rabeprazole. Mechanically, Rabeprazole could inhibit CREB phosphorylation and nuclear translocation, which further decreased the binding of CREB to GPX4 promoter, reducing GPX4 transactivity. Moreover, endogenous PKA interacted with CREB, and this interaction was drastically destroyed in response to rabeprazole treatment. Most importantly, enhanced ferroptosis was observed in H. pylori-infected gastric intestinal metaplasia in comparison to HC control.ConclusionThese findings suggested that rabeprazole induced ferroptosis to reduce CDX2 expression in gastric epithelial cells through PKA/CREB cascade signaling, implying that targeting ferroptosis could be a promising strategy in improving gastric intestinal metaplasia during H. pylori-infected patients.
Suspended soils enrich local forest floor soils during the rainy season in a tropical monsoon rainforest of Hainan Island, South China
Authors: Xu Shitao , Wang Yachen , Yu Xudong , Cai Zeping , Ren Mingxun 
Published: November 2024

IntroductionEpiphytic plants are abundant in rainforests and often serve as traps for litter and dust falling from the canopy. As it accumulates, this material can form nutrient rich soils, which are likely involved in local nutrient cycling and ecological processes.MethodsTo explore spatial and temporal variation in the influence of suspended soils on local nutrient cycles, we compared the physical, chemical and biological properties of suspended soils from the locally-dominant epiphytic Bird’s nest fern (Asplenium nidus L.) to those of three types of forest floor soils (soil collected from upslope, downslope, and underneath the host tree) in a tropical monsoon rainforest in Bawangling National Nature Reserve on Hainan Island, China.ResultsSuspended and forest floor soils were all acidic, with suspended soils having much higher organic matter (66.84%) and water content (~ 300%) than forest floor soils. Suspended soils contained significantly more available nitrogen, phosphorous, and potassium and had much higher urease, cellulase, and catalase activities, indicating that they harbored diverse microbial communities with higher decomposition and biomineralization activity.DiscussionPhysicochemical traits of suspended soil and soil collected from under the host tree were significantly more similar in the rainy season than in the dry season, suggesting that suspended soils may contribute to local nutrient cycling as they are flushed out of epiphytic plants and enrich stemflow and forest floor soils.ConclusionThus, suspended soils play a role in local nutrient cycling, especially during the rainy season. This study provides empirical support for the seasonality and heterogeneity of forest floor soil enrichment by suspended soils in tropical monsoon rainforests.
Trends and determinants of nurses’ mental health following the COVID-19 pandemic in China: a longitudinal, comparative study over a two-year period
Authors: Liu Yan , Lin Yifei , Cui Wenyao , Gu Xianlin , Long Youlin , Liu Wenjie , Liao Ga , Du Liang , Huang Jin 
Published: November 2024

IntroductionThe COVID-19 pandemic has placed nurses at the forefront of healthcare, exposing them to various mental health challenges such as depression, anxiety, and post-traumatic stress disorder (PTSD). However the long-term effects and risk factors of the COVID-19 pandemic on nurses’ mental health are unknown. The objective of our study is to investigate the enduring impact of the COVID-19 pandemic on the psychological distress and PTSD of nurses, while also identifying the factors that influence these outcomesMethodsTo investigate this question, we conducted a self-reported questionnaire survey of 8785 registered nurses recruited using snowball sampling methodology from 22 provinces in China, at five time points (T0-T4). At all times, we took measures of general health, while at the last four times we also measured PTSD. And we used logistic regression analysis to explore their impacts.ResultsThe incidence of whole levels psychological distress among nurses was 27.7% at T0, peaking at 57.6% at T4. For severe psychological distress, it began at 5.5% at T0 and rose to 9.5% at T4. PTSD rates among nurses were recorded at 7.8% in T1, reaching a maximum of 14.7% in T4. They all got progressively worse in the volatility. In all times, coping strategies are independent factors for various levels of psychological distress and PTSD, while social support is a key factor for severe psychological distress and PTSD.ConclusionsOur findings indicated a gradual deterioration in the mental health of nurses following the COVID-19 outbreak. In all instances, coping strategies exhibit an independent correlation with all grades of psychological distress and post-traumatic stress disorder (PTSD), whereas social support emerges as an independent protective factor mitigating the risk of severe psychological distress and PTSD.
Age and gender differences in skin characteristics: a study of 2543 Japanese individuals using advanced skin imaging techniques
Authors: Osuji Yurika , Omatsu Jun , Sato Shinichi , Yoshizaki Ayumi 
Published: November 2024

Skin aging is a multifaceted process influenced by genetic, environmental, and immunological factors, resulting in visible changes such as wrinkles, pigmentation, and alterations in skin texture. Despite its high relevance, there is a paucity of large-scale studies focusing on visible age-related changes in facial skin among Japanese populations, particularly by region. This study aims to address these gaps by examining age- and sex-specific skin changes in a large cohort of 2,543 Japanese subjects using advanced skin imaging techniques. The study included subjects aged 17–71 years who provided informed consent. The imaging system captures facial images using standard light, UV light, and two types of polarized light, applying deep learning techniques to analyze various skin parameters. Statistical analysis was performed to evaluate correlations between age, gender, and skin characteristics. Results indicated significant correlations between age and skin color across all domains, with large pores on the nose showing the highest correlation with age. Sebum and porphyrin levels exhibited a decreasing trend with age, though correlation coefficients were low. Cheek gloss, both in area and color, showed relatively high correlation coefficients. Pigmentation-related items, such as spots and melanin, demonstrated significant age correlations, particularly in areas like the corners of the eyes, under the eyes, and cheeks. Wrinkles and fine wrinkles correlated with age in various regions, though not on the forehead. The findings highlight the importance of understanding regional and demographic variations in skin aging to develop effective anti-aging treatments.
Anti-Inflammatory and Osteogenic Effects of Vitamin K from Sargassum fulvellum Fermented by Lactococcus lactis KCCM12759P and Leuconostoc mesenteroides KCCM12756P
Authors: Sim Yejin , Jo Hyun-Sol , Kim Choong-Gon , Cho Young-Eun , Yang Jungwoo , Hong Sun-Mee 
Published: November 2024

Vitamin K (VitK) is a vital nutrient that is newly recognized to support bone and cardiovascular health. As a nutraceutical, VitK is produced via plant extraction and bacterial fermentation. This study examined the potential anti-inflammatory and osteogenic benefits of VitK, i.e., VitK1 (phylloquinone; PK) and VitK2 (menaquinone; MKs), derived from Sargassum fulvellum fermented by Lactococcus lactis and Leuconostoc mesenteroides (SfLlLm) using lipopolysaccharide (LPS)-induced Raw264.7, MC3T3-E1 cells, and ovariectomized (OVX) mice. MK4, MK7, and MK9, as well as PK, were effectively acquired from SfLlLm and analyzed. SfLlLm_VitK reduced levels of proinflammatory cytokine in LPS-induced Raw264.7 cells and induced an osteogenesis regulating factor in MC3T3-E1 cells. In OVX mice, SfLlLm feeding reduced plasma levels of alkaline phosphatase, phosphate, and the pro-collagen type I alpha 2 gene (pro-Col1a2) while elevating cancellous bone volume and trabecular numbers. Accordingly, SfLlLm, comprising MKs, may be a candidate for preventing and treating immune and bone diseases.
Artificial Induction of Spawning in Threeline Grunt, Parapristipoma trilineatum Under Controlled Environmental Conditions
Authors: Park Jin Woo , Cho Jeong-Hyeon , Baek Seong Il , Im Kang-Hee , Ryu Yong-Woon 
Published: November 2024

The threeline grunt (Parapristipoma trilineatum) is a recently introduced aquaculture species that has attracted considerable interest in Korea. However, research on its aquaculture potential and reproductive biology remains limited. This study aimed to investigate the natural reproductive cycle and the feasibility of manipulating the spawning period of the threeline grunt through environmental control. We designed an environmental changes protocol involving a gradual increase in water temperature (16 °C → 21 °C) and extension of photoperiod (10 L → 14 L) from January to April and compared the group exposed to these conditions to those maintained under natural conditions. The experimental group exhibited significantly higher plasma sex steroid hormone levels at 60 and 90 days and significantly higher gonadosomatic index at 100 days. In addition, the experimental group spawned between April and June, approximately 2 months earlier than that of the control group. Despite earlier spawning, no significant differences were found in egg diameter, hatching rate, and larval notochord length. These findings suggest that spawning can be accelerated through photothermal changes without negatively impacting egg and larval quality. This study provides valuable insights into the reproductive biology of the threeline grunt and highlights the potential of utilizing photothermal control to enhance its aquaculture production.
Observação do manejo da dor em pacientes com fibromialgia em uma cidade no interior da Bahia
Authors: Oliveira Jéssica Santos de , Miranda Mariana Oliveira , Silva Kelle Oliveira 
Published: November 2024

A fibromialgia apresenta uma etiologia incerta e está relacionada a fatores psicológicos como a depressão e a ansiedade. Seu tratamento é diverso necessitando de uma abordagem multidisciplinar, assim o tratamento para fibromialgia ainda carece de mais pesquisas e compreensão de sua propedêutica, já que a persistência dos sintomas afeta a qualidade de vida das pessoas que sofrem com essa síndrome. O presente estudo tem como objetivo identificar as formas terapêuticas farmacológicas e não farmacológicas empregadas em pacientes com fibromialgia e avaliar o possível padrão e o nível da dor que acomete as pessoas que apresentam essa enfermidade. A escolha dos participantes ocorreu por conveniência em uma clínica especializada no tratamento da dor, na cidade de Vitória da Conquista-Ba, no qual foram aplicados 2 questionários, para se obter informações sociodemográficos, perfil clínico e caracterização da dor do participante pelo Questionário McGill da dor em português (QMD – Br). Por meio de uma amostra de 25 pacientes, sendo 96% eram do sexo feminino e 68% casadas, 92% dos entrevistados realizavam terapia farmacológica para o controle da dor, com média de desvio padrão – (DP) ± 0,913. Além do mais, observou-se que índice de dor com média de 26,25 avaliado pelo QMD – Br com prevalência dos descritores como Exaustiva, Intensa, Insuportável e Irradiante. Concluímos que as características das dores da fibromialgia são individualizadas a cada participante, mas que pode predominar certas propriedades, é por conta disso que o tratamento também é adaptado para as necessidades de cada paciente.
Estresse ocupacional e Síndrome de Burnout em médicos que atuam em emergências
Authors: Sousa Ednan Cardoso de , Albuquerque Felipe José de Lima , Duque Luana Caroline Dinelli Oliveira , Lima Gustavo Aurino Queiroz de Lima , Silva Michelle Ribeiro da , Rodrigues Maria Luisa Lopes , Junior Apolonio Alves de Lima 
Published: November 2024

Introdução: A Síndrome de Burnout é o esgotamento da exposição ao estresse ocupacional. Sendo comumente relatado por médicos em todo mundo, com maior prevalência nos médicos de emergência, pois eles experimentam taxas significativamente aumentadas de Burnout. Objetivo: Identificar as evidências científicas produzidas sobre o estresse ocupacional e a Síndrome de Burnout desenvolvidos por médicos que atuam em emergências. Metodologia: Tratou-se de uma revisão integrativa da literatura. Para levantamento de dados científicos, foram utilizadas as bases de dados de artigos indexados em sites de busca como Scielo, LILACS, Pubmed e Medline, dos últimos dez anos. Resultados e Discussão: Após a busca de dados em consonância com os critérios de inclusão e exclusão, desse estudo, foram encontrados 35 artigos e desses utilizados nove. Os estudos utilizados para o escopo dessa pesquisa envolveram pesquisas realizadas na Polônia, Brasil, Canadá, Espanha, EUA, Peru e Sérvia. O desgaste emocional intenso vivenciado pelo profissional em seu trabalho, sendo este uma “extensão do lar”, acarreta ao desequilíbrio harmônico no prazer do que se faz, perdendo o sentido da importância social de sua atividade laboral e para o quotidiano pessoal, com perdas muitas vezes irreparáveis. Conclusão: Estima-se que o presente estudo contribuirá para aprimorar o conhecimento da comunidade científica acerca de estresse ocupacional e o desenvolvimento da Síndrome de Burnout por médicos que atuam nas unidades de emergências.
A Flexible Membrane May Improve Bone Regeneration by Increasing Hydrophilicity and Conformability in Lateral Bone Augmentation
Authors: Lee Dongseob , Ko Young-Chang , Koo Ki-Tae , Seol Yang-Jo , Lee Yong-Moo , Lee Jungwon 
Published: November 2024

Collagen membranes play a crucial role in guided bone regeneration (GBR) by preventing soft tissue infiltration and maintaining space for bone formation. This study investigated the impact of collagen membrane flexibility on GBR outcomes through in vitro and in vivo analyses. Flexible (0.3 mm in width) and stiff (0.5 mm in width) porcine collagen membranes were compared. In vitro tests assessed hydrophilicity, enzymatic degradation, conformability, space maintenance, and tensile strength. An in vivo study using a canine model evaluated bone regeneration in standardized mandibular defects filled with deproteinized porcine bone mineral and covered with no membrane, flexible membrane, or stiff membrane. Micro-computed tomography and histomorphometric analyses were performed at 8 and 16 weeks. The flexible membrane demonstrated superior hydrophilicity, faster enzymatic degradation, and greater conformability in vitro. In vivo, micro-computed tomography analysis revealed similar alveolar ridge widths across all groups. Histomorphometric analysis at 16 weeks showed significantly larger regenerated areas in the flexible membrane group compared to controls in coronal, middle, and apical regions. Both membrane groups exhibited higher regeneration ratios than controls, with significant differences in the coronal area. The flexible membrane group demonstrated significantly higher new bone formation in all regions compared to controls at 16 weeks. These findings suggest that flexible membrane substantially enhances GBR outcomes by increasing hydrophilicity and conformability. The study highlights the potential clinical benefits of incorporating flexible membranes in GBR procedures for improved bone regeneration outcomes.
Deciphering and Targeting the ESR2–miR-10a-5p–BDNF Axis in the Prefrontal Cortex: Advancing Postpartum Depression Understanding and Therapeutics
Authors: Luo Fan , Liu Liming , Guo Mei , Liang Jiaquan , Chen Lei , Shi Xiaojie , Liu Hua , Cheng Yong , Du Yang 
Published: November 2024

Postpartum depression (PPD) represents a important emotional disorder emerging after childbirth, characterized by its complex etiology and challenging management. Despite extensive preclinical and clinical investigations underscoring the role of estrogen fluctuations and estrogen receptor genes in PPD, the precise mechanisms underpinning this condition have remained elusive. In our present study, animal behavioral studies have elucidated a tight link between the aberrant expression of ESR2, miR-10a-5p, and BDNF in the prefrontal cortex of mice exhibiting postpartum depressive-like behavior, shedding light on the potential molecular pathways involved. Integrating bioinformatics, in vivo, and cell transfection methodologies has unraveled the intricate molecular interplay between ESR2, miR-10a-5p, and BDNF. We identified ESR2 as a negative transcription factor that down-regulates miR-10a transcription, while miR-10a-5p serves as a negative regulator that suppresses BDNF expression. This molecular triad contributes to the pathogenesis of PPD by affecting synaptic plasticity, as evidenced by alterations in synapse-related proteins (e.g., SYP, SYN, and PSD95) and glutamate receptor expression. Additionally, primary neuron culture studies have confirmed the critical roles of ESR2 and miR-10a-5p in maintaining neuronal growth and morphology. Therapeutic interventions, including stereotactic and intranasal administration of antagomir or BDNF, have demonstrated significant potential in treating PPD, highlighting the therapeutic implications of targeting the negative transcriptional and regulatory interactions between ESR2, miR-10a-5p, and BDNF. Our findings endorse the hypothesis that estrogen fluctuations and estrogen receptor gene activity are pivotal stressors and risk factors for PPD, affecting central nervous system functionality and precipitating depressive behaviors postpartum.
Realization of CMOS operation in 3-dimensional stacked FET with self-aligned direct backside contact
Authors: Park Juhun , Park Jaehyun , Park Jejune , Hwang Kyuman , Yun Jinchan , Kim Dahye , Park Sungil , Yang Jinwook , Jeong Jae Won , Yun Chuljin , Bae Jinho , Huh Daihong , Yeon Deukho , Kim Sanghyeon , Baek Seungeun , Son Soomin , Lee Junghan , Kim Tae-Sun , Lee Seungjun , Lee Sun-Jung , Park Sang Wuk , Kuh Bong Jin , Ha Daewon , Hyun Sangjin , Ahn Su Jin , Song Jaihyuk 
Published: November 2024

Abstract
Beyond MBCFETTM technology, the 3-dimensional stacked FET (3DSFET) emerges as a promising contender, featuring a structure that stacks NMOS and PMOS vertically to continue area scaling and improving the energy efficiency of logic technology. In this study, we report successful CMOS operations of 3DSFET by implementing vertical integration of a Source/Drain (SD), metal gate, and contacts including Self-Aligned Direct Backside Contact (SA-DBC) in an industry-compatible Contacted Poly Pitch (CPP) of 48 nm.
Development of the 1-D X-ray beam diffuser for multilayer monochromator and its application to X-ray phase tomography
Authors: Hoshino Masato , Uesugi Kentaro 
Published: November 2024

Abstract
The one-dimensional (1-D) X-ray beam diffuser was developed to reduce horizontal stripe-shaped intensity irregularities on the beam from a multilayer monochromator while keeping the horizontal spatial coherence of the beam required for phase measurements using a grating interferometer. The diffused beam showed a smoothed beam profile without degrading the visibility of Moiré fringes in the grating interferometer. In the X-ray phase tomographic measurement, a sectional image with the beam diffuser showed clear structure without artifacts while the sectional image without the diffuser suffered from the remaining striped-shaped artifacts.
AS ATIVIDADES DE ENFERMAGEM NA ASSISTÊNCIA Á MULHER COM DIABETES GESTACIONAL
Authors: Lima Melissa , Rezende da Silva Suelany , Lopes dos Santos Jéssica 
Published: November 2024

A diabetes gestacional é uma doença metabólica que ocorre por conta do aumento da resistência à insulina causada pelos hormônios gestacionais. A diabetes gestacional afeta cerca de 15% das gestações em todo o mundo, no Brasil, 7,6 % das mulheres com mais de 20 anos atendidas no Sistema Único de Saúde são acometidas pela diabetes gestacional, 94% dos casos apresentados apresentam apenas tolerância diminuída à glicose e 6% hiperglicemia no nível de diabetes fora da gravidez. O estudo visa analisar as atividades de enfermagem na assistência á mulher com diabetes gestacional. A metodologia se estrutura na revisão integrativa de literatura, de caráter narrativo e descritivo, com levantamento bibiliográfico nas platarformas Scielo, Biblioteca Virtual de Saúde (BVS) e PubMed, empregou-se na busca os descritores: ações de enfermagem, educação em saúde e cuidados com grávidas diabéticas, delimitando-se ao período de 2019 a 2024. Os estudos apontam para um abordagem terapêutica que consiste em estretégias que combinam a prática de exercícios físicos e monitoramento regular da glicemia, associado principalmente a mudança dietéticas, em alguns casos é necessária a terapia farmacológica. Quanto ás práticas de atividades físicas, estas devem ser apoiadas e incentivadas ao longo da gestação, sempre orientando as pacientes sobre os exercícios que não devem ser realizados e recomenda-se uma duração de 30 minutos ao dia 5 dias por semanas levando em consideração o estado de cada paciente. É impotasnte que as gestantes sejam orientadas a auto monitorar a glicemia de jejum e pós-prandial, mantendo um controle dessas medidas em um diário para ser apresentado ao médico e à equipe da saúde que a acompanha. O auto monitoramento é fundamental para as gestantes compreenderem melhor a influência da alimentação e exercícios nos valores glicêmicos e no bem- estar materno e fetal.