bluuebunny/crossref_metadata_2025_split huggingface dataset:
A reference map of the human proinsulin biosynthetic interaction network
Authors: Tran Duc T. , Pottekat Anita , Mir Saiful A. , Jang Insook , Loguercio Salvatore , Campos Alexandre Rosa , Lahmy Reyhaneh , Liu Ming , Arvan Peter , Balch William E. , Kaufman Randal J. , Itkin-Ansari Pamela 
Published: July 2019

AbstractThe beta-cell protein synthetic machinery is dedicated to the production of insulin, which plays a critical role in organismal homeostasis. Insulin synthesis requires the proper folding and trafficking of its precursor, proinsulin, yet the precise network of proinsulin protein interactions in the secretory pathway remains poorly defined. In the present study we conducted unbiased profiling of the proinsulin interactome in human islets, utilizing a human proinsulin-specific monoclonal antibody for affinity purification and mass spectrometry. Stringent analysis identified a central node of interactions between human proinsulin and sequential secretory pathway proteins that is remarkably conserved across 3 ethnicities and both genders. Among the most prominent proinsulin interactions was with ER-localized peroxiredoxin-4 (PRDX4). A functional role for PRDX4 in beta-cells was demonstrated by gene silencing that rendered proinsulin susceptible to misfolding, particularly in response to oxidative stress. Conversely, exogenous PRDX4 improved proinsulin folding. Notably, oxidative stress and even high glucose treatment alone induced proinsulin misfolding in human islets and MIN6 cells, and this was accompanied by sulfonylation of PRDX4, a modification known to inactivate peroxiredoxins. This finding prompted PRDX4 analysis in a panel of human islet samples that revealed significantly higher levels of sulfonylated (inactive) PRDX4 in islets from patients with T2D compared to that of healthy individuals. Taken together, these data highlight the importance of elucidating the complete proinsulin interactome in human islets in order to understand critical steps controlling insulin biosynthesis, beta cell function, and T2D.
Synthetic mycobacterial molecular patterns partially complete Freund’s adjuvant
Authors: Dubé Jean-Yves , McIntosh Fiona , Zarruk Juan G. , David Samuel , Nigou Jérôme , Behr Marcel A. 
Published: July 2019

AbstractComplete Freund’s adjuvant (CFA) has historically been one of the most useful tools of immunologists. Essentially comprised of dead mycobacteria and mineral oil, we asked ourselves what is special about the mycobacterial part of this adjuvant, and could it be recapitulated synthetically? Here, we demonstrate the essentiality of N-glycolylated peptidoglycan plus trehalose dimycolate (both unique in mycobacteria) for the complete adjuvant effect using knockouts and chemical complementation. A combination of synthetic N-glycolyl muramyl dipeptide and minimal trehalose dimycolate motif GlcC14C18 was able to upregulate dendritic cell effectors, plus induce experimental autoimmunity qualitatively similar but quantitatively milder compared to CFA. This research outlines how to substitute CFA with a consistent, molecularly-defined adjuvant which may inform the design of immunotherapeutic agents and vaccines benefitting from cell-mediated immunity. We also anticipate using synthetic microbe-associated molecular patterns (MAMPs) to study mycobacterial immunity and immunopathogenesis.
Mathematical model of molecular evolution through a stochastic analysis
Authors: Ortega Joel Valdivia 
Published: July 2019

1AbstractToday we are able to describe genome evolution but, there are still several open questions on this area such as: Which is the probability that a mutation occurs at a nucleotide level? Is it possible to predict the evolution of a particular genome?, or talking about preservation, is there a way to simulate the genetic diversity for endangered species? In this paper it is shown that it is possible to make a mathematical model not only of mutations on the genome of species, but of evolution itself, including factors such as artificial and natural selection. It is also presented the algorithm to obtain the probabilities of mutation for each specific part of the genome and for each specie.Even more, it is presented a mathematical method to estimate the amount of generations between two related genomes and a function capable of predict the amount of mutations through time a genome will suffer.The potential of having this tool is giantic going from genetic engineering applied to medicine to filling up blank spaces in phylogenetic studies or preservation of endangered species due to genetic diversity.
Quantifying the surveillance required to sustain genetic marker-based antibiotic resistance diagnostics
Authors: Hicks Allison L. , Kissler Stephen M. , Lipsitch Marc , Grad Yonatan H. 
Published: July 2019

AbstractDiagnostics that minimize the time to selection of an appropriate antibiotic treatment represent an important strategy in addressing the challenge of antimicrobial resistance (AMR). Among this class of diagnostics, the use of pathogen genotype to predict AMR phenotype has been facilitated by advances in rapid sequencing platforms. A longstanding objection to this approach, however, is that the emergence of novel resistance mechanisms will inevitably lead to a decline in the sensitivity of these diagnostics. Here, we show that while the sensitivities of some genetic markers of resistance remain stably high, sensitivities of other markers rapidly decline, as expected, due to the emergence of novel resistance variants. We then present a simple mathematical framework that defines the sampling and phenotypic testing rates needed for early detection of novel resistance variants and thus demonstrate how surveillance can help maintain the sensitivity and utility of sequence-based AMR diagnostics.One sentence summaryTargeted sampling strategies are necessary for early detection of novel resistance mechanisms and sustainability of genotype-based detection of novel resistance mechanisms and sustainability of genotype-based diagnostics.
Slums and prosperity: a complex, dynamic pathway of intervention
Authors: Abubakar Aisha , Romice Ombretta , Salama Ashraf M. 
Published: July 2019

Purpose
Slums have existed as long as cities have and are a growing context in the developing world. The challenge is in their efficient, effective and inclusive management. The purpose of this paper is to frame slums in the broad aim of urban development and the pursuit of prosperity, as active players and positive contributors in every right – a slum-prosperity framework. First, however, they need to be comprehensively defined in a non-exclusive, structured, dynamic and heuristic way; a previous publication was set to resolve this challenge.

Design/methodology/approach
Guided by a synthesized operative model for prosperity, such a definition for slums is used to precisely relate their characters to the pursuit of prosperity through a mapping process, whereby these characters are linked to potentials for prosperity, improvement goals, resources and intervention plans.

Findings
Both slums and prosperity are fuzzy, complex and variable terms; the only possibility to deal with them both is to break them down into simple and manageable yet operative units and establish the most influential and effective links between them to organize intervention according to patterns of change in both slums and city.

Research limitations/implications
An intervention agenda like the one proposed here, that gives room for contextual and temporal urban complexities, has the potential to augment urban practice and help curb the slum phenomenon. A final paper (forthcoming) will illustrate the application of both the comprehensive definition of slum and the implementation of a pathway toward prosperity.

Originality/value
The proposal in this paper is derived as part of research conducted for the award of a PhD at the University of Strathclyde Glasgow. This was in general set to contribute to the proactive and inclusive improvement of slums and cities. The proposal is further derived from the authors’ involvement and personal interest in developing regions and is designed on local experience and on wider expertise in urban renewal.
Rights, duties and spaces of agency amidst high-stakes testing
Authors: Taylor Laura A. 
Published: July 2019

PurposeBy recognizing high-stakes testing as a key constraint to teacher agency, this paper aims to provide a close analysis of one teacher’s testing narrative to illustrate how emerging positioning is relative to high-stakes testing shapes perception of pedagogical agency.Design/methodology/approachData were generated through a series of semi-structured interviews with an early career fourth-grade teacher, Ms Moore, in a school facing pressure to raise test scores. Using theoretical lenses of narrative positioning and a linguistic anthropological centering of constraint and emergence, 67 narratives of accountability were analyzed, with particular focus on how Ms Moore positioned herself relative to other actors involved in high-stakes testing and the consequent rights and duties these positions afforded.FindingsIn narrating the constraints of high-stakes testing, Ms Moore positioned herself relative to three groups involved in high-stakes testing: “purposefully tricky” test creators, “disjointed” administrators and “worried” students. The rights and duties associated with three positions varied with respect to two dimensions – proximity and hierarchy – in turn providing her distinct resources for responding to the pedagogical constraints of high-stakes testing.Practical implicationsTeachers might use positioning analysis as a tool to locate possibilities for agency amidst high-stakes testing, both by exploring the resources afforded by their positioning and by considering how alternative positions might afford different resources.Originality/valueThese findings suggest that high-stakes testing serves as a dynamic and perhaps malleable constraint to teacher agency. Teacher positioning, particularly relative to hierarchy and proximity, provides possible resource for responding to such constraints.
Local government and coastal damage: confusion, potential and dreams
Authors: Kelly Andrew H. , Brown Jasper , Strickland Aaron 
Published: July 2019

Purpose
This paper aims to not only disentangle the recently altered law and policy on coastal management in New South Wales (NSW), Australia, but also raise opportunities for fresh ideas to develop when dealing with both existing and future coastal damage. The focus is on the role of local government which is not only closer to concerned citizens but also faces costal damage on its own doorstep.

Design/methodology/approach
The paper explores the topic from the beginnings of relevant statutory law to the current situation, supported by a case study. It is transdisciplinary in nature, encompassing land use and coastal legislation.

Findings
The narrative encourages further attention to the key issues at the local level. This is underpinned by the need for planners to move beyond zoning and other restrictive mechanisms to more strategic approaches. All levels of government must recognise that regulatory planning on its own is insufficient. This leads to the need for champions to consider opportunities beyond the ordinary.

Originality/value
While this paper will add to a growing literature on coastal damage and action at the local level, its emphasis on the benefits and limitations of the changing statutory system will assist not only policy makers but professional officers at the local forefront.
Developing substantive theories into formal theories via disruption
Authors: Rosenbaum Mark Scott , Russell-Bennett Rebekah 
Published: July 2019

Purpose
The purpose of this paper is to encourage service researchers to engage in “theoretical disruption” by purposefully adding variance to existing substantive theories, and conceptual frameworks, to construct formal theories of buyer–seller marketplace behaviors. The authors put forth an original four-stage process that illustrates the way substantive theories may be developed into formal theories.

Design/methodology/approach
The authors provide their opinions regarding theoretical creation and their interpretations of Grounded Theory methodological techniques that support the development of general theories within the social sciences.

Findings
In general, the services marketing discipline is based on a foundation of substantive theories, and proposed conceptual frameworks, which emerged from samples, contexts and conditions that ensue within industrialized, upper-income locales. Rather than seek to expand substantive theories by generating new categories and relationships between categories, most researchers limit their verification studies within the scope of original theoretical frameworks. Resultantly, the services marketing domain has not developed a set of formal theories.

Research limitations/implications
The editors encourage researchers to reconsider the discipline’s substantive theories and to transform them into formal theories. Substantive theories expand into formal theories when researchers question original theoretical frameworks and show situations in which they require modification. Theoretical verification does not transform substantive theories into formal theories; rather, the discovery of negative cases suggests the need for theoretical modification.

Originality/value
This work suggests that researchers may be over-emphasizing the generalizability of their proposed theories in papers because of a lack of sample variance in empirical studies.
Multiple case study analysis and development of an interpretive structural model for greening of supply chains in Indian ceramic enterprises
Authors: Choudhary Kailash , Sangwan Kuldip Singh 
Published: July 2019

Purpose
The purpose of this paper is to aim at prioritizing and identifying the interrelations among the green supply chain management pressures, practices and performance measures. The study also compares the reasons of green supply chain management implementation, levels of implementation and improvement in performance for medium and large Indian ceramic enterprises because of green supply chain management implementation.

Design/methodology/approach
The most frequent constructs and measures of green supply chain management pressure, practice and performance are identified from the literature. The identified measures are corrected/added/removed, as per the suggestions of industry professionals, to develop a survey instrument. An interpretive structural model is developed to prioritize the constructs. Matriced “impacts croises-multiplication applique” and “classment” (MICMAC) analysis is done to identify the driving and dependence powers of the constructs. The identified results are validated through the descriptive analysis of responses obtained from multiple case studies.

Findings
It is observed that the informative pressures followed by coercive and mimetic pressures are the main drivers for the implementation of green supply chain management practices. Internal environmental management in enterprises is found to be the driving power behind the implementation of other green supply chain management practices. The implementation of these practices drives the environmental and operational performance. It is observed, through the multiple case study analysis that the impact of the pressures to adopt green supply chain management practices is high on Indian ceramic enterprises but the implementation of practices is in the early stage. The improvement in performance is also not found to be significant at this stage. This study also found that large ceramic enterprises are doing better than the medium enterprises in terms of green supply chain management implementation, largely because of higher pressures and availability of resources.

Originality/value
This study prioritizes the constructs of green supply chain management pressure, practice and performance for Indian ceramic enterprises and compares the large and medium enterprises on the basis of these constructs.
Effect of vacuum tumbling and red beetroot juice incorporation on quality characteristics of marinated chicken breast and leg meats
Authors: Singh Prashant , Yadav Sanjay , Pathera Ashok , Sharma Diwakar 
Published: July 2019

PurposeThe purpose of this study was to assess the effect of vacuum tumbling and red beetroot juice (RBJ) incorporation on quality characteristics of marinated chicken breast and leg meat cuts.Design/methodology/approachVacuum tumbling of breast and leg meat cuts was carried for a duration of 2 h and compared with control (0 h tumbling) and traditionally marinated (6 h) meat cuts. Vacuum-tumbled treatments were also marinated using RBJ in place of water to assess the combined effect of RBJ and vacuum tumbling. All the cuts were cooked in an oven at 170°C for 40 min. Quality characteristics of fresh and refrigerated products were analysed.FindingsVacuum tumbling for 2 h resulted in a significant (p < 0.05) increase in marinade pickup, water-holding capacity (WHC), cooking yield, moisture and ash content and a significant (p < 0.05) decrease in hardness, gumminess, chewiness and shear press values in both types of meat. Breast meat treatments had significantly (p < 0.05) higher WHC, moisture, protein, cohesiveness and gumminess and significantly lower fat content, hardness and shear press values than leg meat treatments. Control breast and leg meats had marinade pickup of 9.19 and 9.31 per cent and cooking yield of 69.08 and 68.31 per cent, respectively, whereas corresponding values for treated breast and leg meats varied from 11.28 to 11.98 per cent and 73.32 to 74.94 per cent, respectively. Sensory scores declined significantly (p < 0.05) during refrigerated storage. On the 12th day of storage, control breast and leg meat cuts had acceptability scores of around 5.0, whereas all other treatments had acceptability scores between 6.0 and 7.0 on an eight-point scale, with RBJ-treated products having the highest acceptability. All the products were also microbiologically safe up to the 12th day of refrigerated storage.Originality/valueThe research is valuable to meat processors because vacuum tumbling for 2 h using RBJ as marinade can be used in place of 6 h of traditional marination for developing marinated chicken breast and leg meat products.
Understanding the Flash Crash – state of the art
Authors: Virgilio Gianluca Piero Maria 
Published: July 2019

Purpose
The purpose of this paper is to provide the current state of knowledge about the Flash Crash. It has been one of the remarkable events of the decade and its causes are still a matter of debate.

Design/methodology/approach
This paper reviews the literature since the early days to most recent findings, and critically compares the most important hypotheses about the possible causes of the crisis.

Findings
Among the causes of the Flash Crash, the literature has propsed the following: a large selling program triggering the sales wave, small but not negligible delays suffered by the exchange computers, the micro-structure of the financial markets, the price fall leading to margin cover and forced sales, some types of feedback loops leading to downward price spiral, stop-loss orders coupled with scarce liquidity that triggered price reduction. On its turn leading to further stop-loss activation, the use of Intermarket Sweep Orders, that is, orders that sacrificed search for the best price to speed of execution, and dumb algorithms.

Originality/value
The results of the previous section are condensed in a set of policy implications and recommendations.
Fiscal sustainability in India: evidence from Markov switching and threshold regression models
Authors: Akram Vaseem , Rath Badri Narayan 
Published: July 2019

PurposeThe purpose of this study is to examine the fiscal sustainability issue by dividing the fiscal deficit into high and low regimes using the quarterly data from 1997: Q1 to 2013: Q3. Further, we obtain the optimum level of public debt at which fiscal sustainability can be achieved.Design/methodology/approachThis study uses the Markov Switching-Vector Error Correction Model (MS-VECM) for examining fiscal sustainability and threshold regression model to obtain the optimum level of debt.FindingsThe results derived from MS-VECM reveal the evidence in favor of fiscal sustainability during low fiscal deficit periods. Similarly, using a threshold regression model, the optimum public debt as a percentage to GDP seems to be around 21 per cent on a quarterly basis, beyond this level, public debt hurts economic growth.Practical implicationsFrom the policy front, the government of India should cut down the fiscal deficit only if debt reaches beyond a threshold level.Originality/valueNoting that the vast literature has focused on examining the fiscal sustainability in India, the novelty of this study is to examine the fiscal sustainability by considering high and low deficits regimes using a non-linear approach.
Laparoscopic stapled resection/plication of left hemi‐diaphragmatic eventration in an adult
Authors: Premchandani Dhiraj , Chitnis Akshay , Katara Avinash , Bhandarkar Deepraj 
Published: July 2019

AbstractWe report a 59‐year‐old man who presented with progressive dyspnea of 30 years. His pulmonary function tests showed reduced forced expiratory volume in the first second and forced vital capacity and computed tomography of the chest showed eventration of the left hemi‐diaphragm. He underwent successful laparoscopic stapled resection / plication of the lax hemi‐diaphragm. He was discharged on the fourth postoperative day with the intercostal drain in situ and this was removed 5 days later at follow up. Postoperatively the pulmonary function showed significant improvement and he remains well 2 years after surgery.
Beyond stereotypes of cerebral palsy: Exploring the lived experiences of young Canadians
Authors: Hanes Julia E. , Hlyva Oksana , Rosenbaum Peter , Freeman Matthew , Nguyen Tram , Palisano Robert J. , Gorter Jan Willem 
Published: July 2019

AbstractBackgroundHealth for people with cerebral palsy (CP) must extend beyond physical impairments to include social, environmental, and psychological factors that are rarely captured by quantitative research alone. This qualitative study sought to explore the lived experience of young people with CP with their physical, mental, and emotional health in the context of a larger longitudinal Canadian study focusing on brain function, physical and mental health, and well‐being.MethodsAn integrated research team (including people with CP or other impairments, clinicians, and researchers) was formed to study participant‐identified research needs. A purposive sample of 16 people with CP (seven female), aged 17–29, Gross Motor Function Classification System (GMFCS) levels I–V, participated in three focus groups that were conceptualized and analysed using interpretive description methodology.ResultsThis study reports the experiences of people with CP across GMFCS levels and identifies some consequences of growing up with the condition: physical and mental health issues, importance of meaningful participation, impact of the environment, and identity formation. Participants shared challenges related to accessibility, healthcare, social/environmental supports, relationships, and sustainable employment.DiscussionBody structure and function challenges impact participation in activities of daily living, threatening participants' ability to form positive identities and live meaningful lives. People with CP desire to work but may require additional training, accommodation, and support to do so. Environmental conditions, including relationships, supportive people, and accessibility, shape participants' health, well‐being, and social/civic engagement. This study confirms the need for improved care for adults with CP, including multidisciplinary adult health team(s) and community services.