bluuebunny/crossref_metadata_2025_split huggingface dataset:
A dual motif mediates outer-membrane translocation and packing of glycosidases intoBacteroidesOuter Membrane Vesicles
Authors: Valguarnera Ezequiel , Scott Nichollas , Feldman Mario F. 
Published: July 2018

ABSTRACTOuter membrane vesicles (OMV) are spherical structures derived from the outer membrane (OM) of Gram-negative bacteria.Bacteroides spp. areprominent components of the human gut microbiota, and OMV produced with these species are proposed to play key roles in gut homeostasis. OMV biogenesis inBacteroidesis a poorly understood process. Here, we revisited the protein composition ofB. thetaOMVs by mass spectrometry. We confirmed that OMVs produced by this organism contain large quantities of glycosidases and proteases, with most of them being lipoproteins. We found that most of these OMV-enriched lipoproteins are encoded by polysaccharide utilization loci (PULs), such as thesusoperon. We examined the subcellular localization of the components of the Sus system, and found that the alpha-amylase SusG is highly enriched in OMVs while the oligosaccharide importer SusC remains mostly in the OM. We show that all OMV-enriched lipoproteins possess a lipoprotein export sequence (LES) that mediates translocation of SusG from the periplasmic face of the OM towards the extracellular milieu and is required for SusG to localize preferentially to OMVs. We also show that surface-exposed SusG in OMVs is active and can rescue growth of bacterial cells incapable of growing on starch as only carbon source. Our results support the role of OMVs as “public goods” that can be utilized by other organisms with different metabolic capabilities.IMPORTANCESpecies from theBacteroidesgenus are predominant members of the human gut microbiota. OMVs inBacteroideshave been shown to be important for the homeostasis of complex host-commensal relationships, mainly involving immune tolerance and protection from disease. OMVs carry many enzymatic activities involved in the cleavage of complex polysaccharides and have been proposed as public goods that can provide growth to other bacterial species by release of polysaccharide breakdown products into the gut lumen. Nevertheless, the mechanistic nature of OMV biogenesis is unclear forBacteroidesspp. This works shows the presence of a negatively-charged rich amino acid dual motif that is required for efficient packing of the surface-exposed alpha-amylase SusG into OMVs. Discovery of this motif (LES) is the first step in the generation of tailor made probiotic interventions that can exploit LES-related sequences to generateBacteroidesstrains displaying proteins of interest in OMVs.
Human cytomegalovirus RNA2.7 regulates host cell cycle and facilitates viral DNA replication by inhibiting RNA polymerase II phosphorylation
Authors: Huang Yujing , Zhang Jing , Guo Xin , Wang Qing , Liu Zhongyang , Ma Yanping , Qi Ying , Ruan Qiang 
Published: July 2018

AbstractHuman cytomegalovirus (HCMV) is a ubiquitous pathogen belongs to the beta herpesvirus family. RNA2.7 is a viral long non-coding RNA accounting for more than 20% of total viral transcripts at early time of infection. By construction of RNA2.7 deleted mutant and genome transcriptomic analysis, RNA2.7 is demonstrated to repress host cellular RNA polymerase II (Pol II)-dependent transcription through inhibiting the phosphorylation of RNA polymerase II (Pol II). Co-immunoprecipitation, RNA immunoprecipitation and RNA electrophoretic mobility shift assay are followed to investigate its mechnism. A 145nt-in-length fragment in RNA2.7 is identified to bind to Pol II and block the interaction between Pol II and phosphorylated cyckin-dependent kinase 9 (phospho-CDK9). By inhibiting Pol II phosphorylation, RNA2.7 decreases the transcription and expression levels of chromatin licensing and DNA replication factor 1 (Cdt1) and cell division cycle gene 6 (Cdc6). Through above way, RNA2.7 prevents the entry of cells into S phase and facilitates viral DNA replication. Our results discover the functions of HCMV RNA2.7 in regulation of Pol II phosphorylation and cell cycle control during infection.Author summaryHuman cytomegalovirus (HCMV) RNA2.7 is a viral lncRNA that is most abundant during infection. Here we show that a 145nt-in-length fragment in RNA2.7 binds to RNA polymerase II (Pol II) and blocks the interaction between Pol II and phosphorylated cyckin-dependent kinase 9 (phospho-CDK9). By inhibiting Pol II phosphorylation, RNA2.7 decreases the transcription and expression levels of chromatin licensing and DNA replication factor 1 (Cdt1) and cell division cycle gene 6 (Cdc6), and blocks host cells entering into S phase. RNA2.7 is confirmed to facilitate viral DNA replication through decreasing Cdt1 and Cdc6. Therefore, our results discover the functions of HCMV RNA2.7 in regulation of Pol II phosphorylation and cell cycle control during infection.
The Future Museum shapes the museum future
Authors: Wang Michelle Chaotzu , Lin James Quo-Ping 
Published: July 2018

Purpose
The purpose of this paper is to introduce a progressive strategy of the National Palace Museum (NPM) using new media art exhibitions as a creative marketing tool to interpret its collection, generate cultural value and navigate the greater global context.

Design/methodology/approach
A review of museum marketing literature and the challenges presented by Taiwan’s sociopolitical situation contextualize discussions on marketing activities and the emergence of museum-commissioned new media art at the NPM within the past two decades.

Findings
Democratic potency inherent in the digital medium has enabled the NPM to transcend the conflicting cultural perceptions surrounding its collection and fulfill the function of market expansion and cultural transmission.

Originality/value
Specialized heritage museums, such as the NPM, do not traditionally create or collect contemporary artworks that engage in ongoing cultural dialogues. This paper brings into view the novelty of using the digital medium to generate cultural value as exemplified in the new media art commissioned by the NPM.
Which consumers opt for organic wine and why? An analysis of the attitude-behaviour link
Authors: Schäufele Isabel , Pashkova Daria , Hamm Ulrich 
Published: July 2018

Purpose
The purpose of this paper is to analyse the effect of attitudes and socio-demographics on wine consumers’ real purchase behaviour for organic wine.

Design/methodology/approach
This study is based on GfK household panel data, a real market data source of high population coverage. A two-part fractional model was applied as two distinct categories of wine buyers were observed. The first part of the two-part fractional model consisted of a standard binary choice model and defined the likelihood of belonging to the group of organic wine buyers. The second part of the model only took organic wine buyers into account and described their purchase intensity.

Findings
Preferences for organic products and sustainability concerns (e.g. environmental and social concerns) drive organic wine purchases. Proving a causal relation between attitudes and purchase behaviour gives evidence that stated preferences are a reliable indicator to predict consumer behaviour. However, the weak relation between attitudes and behaviour confirms the existence of an attitude-behaviour gap.

Practical implications
Quality benefits of organic wine production need to be communicated to attract new customers. Stronger focus should be put on sustainability issues with the aim of encouraging organic customers to also increase their expenditures for organic wine.

Originality/value
The influence of sustainability concerns on purchase behaviour is still controversial and no study, so far, has analysed real purchase data for organic wine. The results provide new insights on why attitudes do not fully transform into purchase behaviour.
Fostering knowledge sharing and knowledge utilization
Authors: Ouakouak Mohammed Laid , Ouedraogo Noufou 
Published: July 2018

PurposeThe purpose of this paper is to explore the influence of organizational commitment and trust on knowledge sharing and on knowledge utilization. Also, the study aims to examine the influence of knowledge sharing on knowledge utilization.Design/methodology/approachA quantitative study was conducted among 307 employees working at Canadian organizations.FindingsThe results reveal that both affective commitment and professional trust have positive influences on knowledge sharing and knowledge utilization, whereas personal trust and continuance commitment do not. The authors also found that business ethics moderates the relationship between knowledge sharing and knowledge utilization.Practical implicationsThese findings extend the literature on knowledge management and demonstrate, from a practical perspective, that in order to build a knowledge-sharing culture, managers must create conditions that allow affective commitment, professional trust and business ethics to flourish.Originality/valueThe current study offers an initial investigation of the effects of both kinds of commitment and trust on knowledge sharing and knowledge utilization.
Relative leader-member relationships within group context
Authors: Bakar Hassan Abu , Omilion-Hodges Leah M. 
Published: July 2018

PurposeAlthough the importance of group leader and group member dyadic relationships has been increasingly emphasized, only few studies have focused on the dyadic level analysis of leader–member relationships. By integrating theories of relational leadership and relational dyadic communication among workgroups, the purpose of this paper is to propose a theoretical model that links relative leader–member exchange quality (RLMX) and relative leader–member conversation quality (RLMCQ) to group performance, as mediated by group cooperation.Design/methodology/approachThe model was tested in a field study with multiple sources, including 232 leader–member dyads and 407 workgroup peer dyads among 70 intact workgroups. Data were collected on-site during paid working hours from four training sessions. Group members were surveyed four times (Time 1, Time 2 and Time 3) and group leaders were surveyed once (Time 4) to minimize common method bias. The hierarchical linear modeling and polynomial regression approach were used to determine the mediating effects of the group cooperation.FindingsIn this study, the authors found support for indirect effects of relative RLMX and RLMCQ on group performance through the mediating role of group cooperation.Research limitations/implicationsThe cross-sectional design of the current study is to be interpreted with caution, concerning any conclusions about the causal ordering of the variables in the model.Practical implicationsIn organizational situations with group leaders and group members already in high-quality relationships and conversation, management should endeavor to facilitate opportunities for cooperation among group members and a means to also enhance team–member exchange.Originality/valueBy introducing LMCQ and group member cooperative behavior in workgroups, this study actively respond to the scholars’ warnings that ignoring the workgroup context may hamper the progress in understanding the factors that will inhibit or enhance workgroup behavior.
Internationalisation at home for tourism training programmes: case studies from Vietnam
Authors: Khuong Cam Thi Hong , Thi Tran Ly 
Published: July 2018

Purpose
Tourism is one of the most notable features of the contemporary globalised world. The tourism industry is becoming increasingly vital to the economy of many developing and developed countries around the globe. The demand of the tourism industry has posed a challenge for tourism training providers to move towards a more responsive and internationalised curriculum to enhance work readiness for tourism graduates who are expected to work with an increased number of international tourists. The purpose of this paper is analyse whether and how internationalisation has been implemented in the tourism training programmes across six institutions in Vietnam.

Design/methodology/approach
The research deployed case studies as research strategy with interviews and document analysis as two instruments of data collection.

Findings
The major findings show that even though the tourism industry demands graduates to possess global competency, knowledge and skills, the curriculum does not prioritise the internationalisation dimensions and the faculty members are not facilitated to be internationally active in their roles. Overall, internationalisation is still fragmented and ad hoc in these institutions even though the private institutions in this research appear to be more responsive to the trend of internationalisation in education than their public counterparts.

Research limitations/implications
The paper provides recommendations on how to effectively embed internationalisation components into local tourism training programs in Vietnam.

Originality/value
The research bridges the gap in the literature on internationalisation of the local tourism programme in non-English-speaking countries.
Development and characterization of three-dimensional woven fabric for ultra violet protection
Authors: Nasreen Adeela , Umair Muhammad , Shaker Khubab , Hamdani Syed Talha Ali , Nawab Yasir 
Published: July 2018

Purpose
The purpose of this paper is to investigate the effect of materials, three dimensional (3D) structure and number of fabric layers on ultraviolet protection factor (UPF), air permeability and thickness of fabrics.

Design/methodology/approach
Total 24 fabrics samples were developed using two 3D structures and two weft materials. In warp direction cotton (CT) yarn and in weft direction polypropylene (PP) and polyester (PET) were used. Air permeability, thickness and UPF testings were performed and relationship among fabric layers, air permeability, thickness and UPF was developed.

Findings
UPF and thickness of fabrics increases with number of fabric layers, whereas air permeability decreases with the increase in number of fabric layers. Furthermore, change of multilayer structure from angle interlock to orthogonal interlock having same base weave does not give significant effect on UPF. However, change of material from polyester (PET) to polypropylene (PP) has a dominant effect on UPF. Minimum of three layers of cotton/polyester fabric, without any aid of ultraviolet radiation (UV) resistant coating, are required to achieve good. Cotton/polyester fabrics are more appropriate for outdoor application due to their long-term resistance with sunlight exposure.

Originality/value
Long-term exposure to UV is detrimental. So, there is need of proper selection of material and fabric to achieve ultraviolet protection. 3D fabrics have yarns in X, Y as well as in Z directions which provide better ultraviolet protection as compared to two dimensional (2D) fabrics. In literature, mostly work was done on ultraviolet protection of 2D fabrics and surface coating of fabrics. There is limited work found on UPF of 3D woven fabrics.
From good to great: nonlinear improvement of healthcare service
Authors: Liu Sandra , Chen Jie , Sun Zhaonan , Zhu Michael Yu 
Published: July 2018

Purpose
Increasing consumer choices in health care results in keener competition faced by providers. The existing nonlinear and asymmetric impact of patient perceived quality of care to their choices should provide insights for hospitals to deploy limited resources to areas that produces most significant and positive outcomes. This study aims to develop an algorithm for examining the nonlinear and asymmetrical relationships in the health-care domain with the hope to provide a more precise indication as to how specifically addressing patient experience with meaningful improvements of service quality.

Design/methodology/approach
Data were obtained through telephone surveys of 2,000 inpatients who had been hospitalized in a health system in 2006. After coding the original data, the authors conducted regression analysis and Z-test to investigate the nonlinear and asymmetrical relationship between patient recommendation and perceived hospital attributes.

Findings
“Spiritual care” is considered as a “delight” for patients. “Procedure efficiency”, “Compassionate care” and “Mutual communication” linearly related to patients’ likelihood to recommend the hospital (LTR) in the positive domains, but increasingly sensitive to LTR in negative domain.

Practical implications
Examining the asymmetry and the nonlinear relationship can detect diminishing effect of certain drivers for patient satisfaction. An emphasis on patients’ spiritual needs can provide the hospital with a unique opportunity to differentiate itself from other health-care providers which usually compete within the ordinary domain of services. The absence of “Procedure efficiency”, “Compassionate care” and “Mutual communication” will result in extremely negative word-of-mouth.

Originality/value
This study has developed an algorithm to examine the asymmetry and the nonlinear relationship between perceived hospital performance and patient satisfaction. The insights generated should help providers determine specific sets of priorities for improving services and hence strategize for optimal deployment of limited resources.
Quality management systems as indicators for stability and change in customer-supplier relationships
Authors: Hallberg Peter , Hasche Nina , Kask Johan , Öberg Christina 
Published: July 2018

Purpose
This paper extends the discussion on stability and change through focus on specific relationship characteristics. Quality management systems prescribe established routines for supplier selection and monitoring, and may thereby designate the nature and longevity of customer–supplier relationships. The purpose of this paper is to describe and discuss the effects of quality management systems on stability and change in different forms of customer–supplier relationships.

Design/methodology/approach
A number of illustrative examples based on participatory data and interviews help to capture different types of customer–supplier relationships (private/public; certified/non-certified) related to quality management systems.

Findings
While certified customers in most sectors only need to prove that their suppliers have procedures in place, many customers equate this with requiring that their suppliers should be certified. The paper further shows that customers replace deeper understandings for their suppliers’ procedures with the requirement that they be certified.

Originality/value
The paper contributes to the existing literature through integrating quality management systems literature with the business network approach. For business network studies, the discussion on quality management systems as constricting regimes is interesting and provides practical insights to the business network studies as such quality management systems increase in importance and spread.
Analysis of barriers to women entrepreneurship through ISM and MICMAC
Authors: Tripathi Kumari Amrita , Singh Saumya 
Published: July 2018

PurposeThis paper aims to study the impediments and difficulties that prevent Indian women from becoming entrepreneurs.Design/methodology/approachData were obtained through a survey involving 15 experts. Based on the feedback provided by the experts, ten relevant barriers in the context of Indian micro small and medium enterprises (MSMEs) were chosen. A structured questionnaire was used to gather data. These ten barriers create obstruction for Indian women as entrepreneurs. These barriers were ranked, and causal relationships among them established using interpretive structural modeling andMatrice d’Impacts croises-multiplication appliqúean classment(cross-impact matrix multiplication applied to classification) (ISM–MICMAC) approach.FindingsThis study identifies, on the basis of extant literature and experts’ opinion, ten barriers to female entrepreneurship. These barriers were ranked, and causal relationships among them established using the ISM–MICMAC approach. On the basis of ranking, women can move forward in MSMEs after removing these obstacles and it will have good results.Research limitations/implicationsIn this research, with literature reviews and experts opinion, ten barriers have been identified for women’s entrepreneurship and have been used to build the model.Practical implicationsTo bring Indian women forward in the field of entrepreneurship, both the society and the government should work together, and efforts should be made to overcome the obstacles coming in the way of entrepreneurs.Social implicationsFemale entrepreneurship in India faces many problems including negative attitude of authorities and society toward women. The society and authorities have no format or model for Indian women to move forward in the entrepreneurship sector.Originality/valueThis study seeks to identify, on the basis of a thorough review of literature and expert opinion, major barriers to female entrepreneurship in the context of Indian MSMEs.
Evaluating the cost and benefits of earthquake protection of buildings
Authors: Manganelli Benedetto , Vona Marco , De Paola Pierfrancesco 
Published: July 2018

Purpose
The purpose of this study is the evaluation of the cost and benefits of earthquake protection of buildings to verify whether the legislative push, through tax incentives, will produce results and lead to a redevelopment of private real estate assets.

Design/methodology/approach
Through contingent valuation, this research aims to measure the propensity of homeowners to invest in the seismic security of their properties. The sample of homeowners was selected in a southern Italy city, which was characterized by a medium-high seismic hazard. The willingness to pay, once made independent from the family income, was compared with the actual cost of a seismic retrofitting technique to assess its cost-effectiveness.

Findings
The analysis developed on an example case shows that the economic sustainability of the intervention is only verified when considering the current tax incentives for this type of intervention.

Practical implications
Choosing to introduce a system to compulsory insurance against seismic risk could certainly be a strong incentive for the implementation of retrofitting interventions on private real estate assets. In this direction, investigations like this can be fundamental to establish the fair risk premium.

Originality/value
The need for effective seismic risk mitigation policies is also based on the growing awareness of the, often fatal, effects of seismic events, emphasized by the recent medium and high intensity events that hit Italy. The issue of the security of residential buildings is therefore a very topical issue in view of their high seismic vulnerability and the vast number of buildings requiring major seismic retrofitting. Therefore, the propensity of owners to intervene in improving the seismic performance of their properties can be crucial in seismic risk mitigation.