bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery of a Novel Public Antibody Lineage Correlated With Inactivated SARS‐CoV‐2 Vaccine and the Resultant Neutralization Activity
Authors: Wang Jing , Ni Shuangshuang , Chen QianQian , Wang Chenchen , Liu Hao , Huang Lina , Nasir Muhammad Waqas , Wang Wei , Zhang Xiangyu , Wu Jianjun , Liu Zhirong , Wu Jiabing , Zhang Liangmin , Gao Yong 
Published: November 2024

ABSTRACTWhile the SARS‐CoV‐2 vaccine offers 70%–95% protection effectiveness against Coronavirus disease 2019 (COVID‐19), a portion of recipients do not produce adequate protective immune responses, particularly, neutralizing antibodies (nAbs). Previous studies of COVID‐19 patients have identified several public antibody lineages, such as IGHV3‐30, IGHV3‐33, IGHV3‐53, IGHV1‐58, and IGHV1‐24. However, it remains unclear how these public antibodies evolve during vaccination or whether there are any special antibody lineages correlated with SARS‐CoV‐2 vaccination. In this study, through a combination of single B cell sequencing and next‐generation sequencing analysis, we systemically studied the dynamic changes of antibody lineages derived from different B cell germlines in their sequence, frequency, and neutralization ability in different vaccinees before and after receiving inactivated SARS‐CoV‐2 vaccines. Our findings indicate that the frequency of antibodies derived from the IGHV4‐34 lineage increased in most individuals after vaccination, and the higher frequency of the antibody usually resulted in stronger binding affinity. Additionally, the ratio of IGHV4‐34 derived antibodies, when compared with other public antibodies, more strongly correlated with the neutralization activity of immune sera from vaccinees. Taken together, these results suggest that IGHV4‐34 is a novel vaccine‐elicited public nAb lineage that plays a crucial role in immune response following inactivated COVID‐19 vaccination.
Association Between NK Cell Genetic Variants and the Development of Long COVID Associated‐ and Prepandemic Small Fiber Neuropathy
Authors: Graninger Marianne , Endmayr Verena , Kühner Laura M. , Berger Sarah M. , Rommer Paulus , Klotz Sigrid , Gelpi Ellen , Vietzen Hannes , Höftberger Romana , Puchhammer‐Stöckl Elisabeth 
Published: November 2024

ABSTRACTLong coronavirus disease 2019 (COVID) (LC) symptoms including pain and autonomic dysfunction are in some patients associated with small‐fiber neuropathy (SFN). The pathomechanisms underlying SFN are mostly unclear. Natural killer (NK) cells play a crucial role in immune regulation, viral clearance and nerve metabolism. The aim of this study was to identify associations between development of small‐fiber dysfunction dependent and independent of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection, and human genetic markers associated with specific NK cell functions. The genetic markers assessed in all cohorts included: FCGR3A, IGHG1, HLA‐E, NKG2C, and rs9916629. Genotyping was performed using TaqMan assays, Sanger sequencing and touchdown polymerase chain reaction. We assessed human cytomegalovirus (HCMV) IgG serostatus in all participants, and screened for anti‐neuronal, anti‐glial and anti‐ganglioside autoantibodies in both patient cohorts. We included 50 LC patients with newly‐emerged symptoms of small‐fiber dysfunction after SARS‐CoV‐2 infection, 27 prepandemic SFN patients and 320 control persons. Markers associated with low NKG2C response, that is, deletion of the NKG2C gene and lack of prior HCMV infection (IgG seronegativity), occurred significantly more frequently in prepandemic SFN patients compared to LC patients and controls (p = 0.0109 and 0.0005, respectively). In conclusion, markers of impaired NKG2C pathways are associated with prepandemic SFN, but not with Long COVID‐associated small‐fiber dysfunction.
Decreased Water Diffusivity Along the Perivascular Space in Older Adults With Poor Sleep Quality
Authors: Kikuta Junko , Kamagata Koji , Takabayashi Kaito , Hayakawa Yayoi , Taoka Toshiaki , Saito Yuya , Uchida Wataru , Guo Sen , Yoshida Seina , Yamazaki Keigo , Wada Akihiko , Kaga Hideyoshi , Tamura Yoshifumi , Kawamori Ryuzo , Watada Hirotaka , Aoki Shigeki 
Published: November 2024

ABSTRACTThis study included 52 Japanese older adults with Pittsburgh Sleep Quality Index (PSQI) scores > 5 and 52 healthy controls (HCs) with PSQI score ≤ 5. Diffusion‐weighted imaging (DWI) and 3D T1‐weighted imaging were acquired using 3T magnetic resonance imaging. The diffusion tensor image analysis along the perivascular space (DTI‐ALPS) index was calculated using preprocessed DWI. The choroid plexus volume (CPV) was calculated using FreeSurfer 6.0. The mean ALPS index and CPV were compared between the older adults with poor sleep quality (PSQ) and HCs using a general linear model, adjusted for covariates including age, sex, years of education, total intracranial volume, systolic blood pressure, hemoglobin A1c, and white matter lesion volume. We also conducted a partial correlation analysis between the mean ALPS index and CPV, Montreal Cognitive Assessment (MoCA), and PSQI scores, adjusting for all the mentioned covariates. The PSQ group had a significantly lower mean ALPS index than HCs. The mean ALPS index in the PSQ group was negatively correlated with CPV and positively correlated with the MoCA score. Therefore, older adults with PSQ may experience dysfunction in the excretory pathway of the perivascular space around the medullary veins. This impairment may be associated with an increase in CPV and cognitive dysfunction.
A Method for Identifying Warm Fronts in Eurasia
Authors: Qin Yujing , Wang Jiachen , Lu Chuhan , Feng Mengru 
Published: November 2024

ABSTRACTWarm fronts often trigger significant weather changes, which also play a role in many extreme weather incidents. Therefore, it is crucial to understand the location and characteristics of warm fronts to accurately forecast weather changes. However, warm fronts are more difficult to identify than cold fronts on average, since the gradients can be weaker and shallower. This paper proposes a new objective method for identifying warm fronts in the Eurasia region using ERA‐5 hourly reanalysis data. The method uses the appropriate thermal front parameter and warm advection threshold to identify the potential warm frontal zone, then determines the corresponding warm boundary according to the predominant wind direction within the frontal zone, and finally locates the warm front line along the warm boundary. In various weather processes, the location and shape of the objective warm front correspond well with the distribution of weather systems and meteorological elements, indicating the effectiveness of the method. Meanwhile, the high agreement between objective and manual warm fronts further supports the reliability of the method. Furthermore, this method is applied to the long‐term datasets covering Eurasia, enabling an exploration of the climatological characteristics of warm fronts in the region. The study reveals distinct seasonal patterns in warm front frequency, with the highest frequency occurring during winter and the lowest during summer. Warm fronts are notably active in Europe, the Siberian Plain, the Northeast China Plain extending to the Western Pacific during winter, spring, and autumn. The dataset of warm fronts produced by this method proves valuable for climate change research.
Low intensity, high frequency vibration training to improve musculoskeletal function in a mouse model of volumetric muscle loss
Authors: Hoffman Daniel B. , Schifino Albino G. , Cooley Marion A. , Zhong Roger X. , Heo Junwon , Morris Courtney M. , Campbell Matthew J. , Warren Gordon L. , Greising Sarah M. , Call Jarrod A. 
Published: November 2024

AbstractThis study's objective was to investigate the extent to which two different levels of low‐intensity vibration training (0.6 g or 1.0 g) affected musculoskeletal structure and function after a volumetric muscle loss (VML) injury in male C57BL/6J mice. All mice received a unilateral VML injury to the posterior plantar flexors. Mice were randomized into a control group (no vibration; VML‐noTX), or one of two experimental groups. The two experimental groups received vibration training for 15‐min/day, 5‐days/week for 8 weeks at either 0.6 g (VML‐0.6 g) or 1.0 g (VML‐1.0 g) beginning 3‐days after induction of VML. Muscles were analyzed for contractile and metabolic adaptations. Tibial bone mechanical properties and geometric structure were assessed by a three‐point bending test and microcomputed tomography (µCT). Body mass‐normalized peak isometric‐torque was 18% less in VML‐0.6 g mice compared with VML‐noTx mice (p = 0.030). There were no statistically significant differences of vibration intervention on contractile power or muscle oxygen consumption (p ≥ 0.191). Bone ultimate load, but not stiffness, was ~16% greater in tibias of VML‐1.0 g mice compared with those from VML‐noTx mice (p = 0.048). Cortical bone volume was ~12% greater in tibias of both vibration groups compared with VML‐noTx mice (p = 0.003). Importantly, cross‐section moment of inertia, the primary determinant of bone ultimate load, was 44% larger in tibias of VML‐0.6 g mice compared with VML‐noTx mice (p = 0.006). These changes indicate that following VML, bones are more responsive to the selected vibration training parameters than muscle. Vibration training represents a possible adjuvant intervention to address bone deficits following VML.
Changes in the freshness of freshwater Japanese icefish (Salangichthys microdon) during chilled and super‐chilled storage
Authors: Ando Masashi , Kubo Hayato , Mok Wen Jye , Itoh Ichiro , Imaizumi Kensaku , Tanaka Teruyoshi , Fukuda Takashi 
Published: November 2024

AbstractBackgroundThe Japanese icefish (Salangichthys microdon) is a valuable food resource in Japan; however, its freshness rapidly diminishes during transport and cold storage. In this study, the freshness change during chilled (5°C) and super‐chilled (−1 and −2°C) of S. microdon samples collected from Kasumigaura lake in October and December was assessed by observing their surface structure and determining their drip loss, K values, and free amino acid content.ResultsThe results revealed that more wrinkles formed on the surface of S. microdon samples with increasing storage time. More drip loss occurred in fall than in winter. Higher K values and increased degradation of muscle proteins were observed in winter. Moreover, their free amino acid content increased with storage time at all temperatures.ConclusionThis study suggested that seasonal differences and storage temperature should be considered in selecting the appropriate fishing, handling, and storage methods to maintain the freshness and quality of S. microdon.
Expression, characterization and application of the deoxynivalenol dehydrogenase from Devosia yakushimensis
Authors: Xu Wei , Yao Jiayi , Shi Yan , Zhang Wenli , Guang Cuie , Mu Wanmeng 
Published: November 2024

AbstractBACKGROUNDDeoxynivalenol (DON) is one of the type‐B trichothecene mycotoxins generally produced by Fusarium fungi and widely pollutes wheat and other grains, causing a great loss in grain yield and significant threat to human health. Enzymatic treatment of DON has displayed a significant role in controlling its levels in recent years.RESULTSHere, the DON dehydrogenase from Devosia yakushimensis (DeDDH) is reported. DeDDH exhibited the highest activity in an environment of 35 °C and pH 7.5. In addition, the residual activity of DeDDH was about 40% after pre‐incubation at 80 °C for 0.5 h, suggesting a good thermostability. The presence of Ca2+ significantly boosted the activity of DeDDH, but substituting Ca2+ with other ions could hardly retain the activity. At the optimal conditions, DeDDH efficiently degraded 75% DON (45 μg mL−1) within 4 h. Notably, when three cofactors, PQQ, PMS and DCPIP, were added together, more than 90% DON (45 μg mL−1) was quickly degraded in the first 40 min.CONCLUSIONThe residues Thr485 and Leu521 were found to be significant to the activity of DeDDH by site‐directed mutagenesis, much different from other DON dehydrogenases. Nevertheless, further research on the crystal structure determination of DeDDH, key catalytic residue identification, catalytic activity and thermostability modification still needs to be conducted for application in industry. © 2024 Society of Chemical Industry.
Medicinal potential of adventitious root cultures of Hypericum perforatum: study on antioxidant stress and anti‐melanogenic properties
Authors: Guo Yu‐Qing , Li Xue‐Feng , Zhang Zhao , Li Han‐Xi , Zhang Ke‐Xin , Zhou Zhong‐Yu , Piao Xuan‐Mei , Lian Mei‐Lan 
Published: November 2024

AbstractBACKGROUNDAdventitious root (AR) culture is an effective method for the production of Hypericum perforatum raw materials. However, the ARs are seldom utilized in practical applications. Therefore, this study investigated the effects of H. perforatum ARs on antioxidative stress and anti‐melanogenesis, aiming for future applications in the development of related products.RESULTSIn the antioxidative stress experiment, tert‐butyl hydroperoxide‐stimulated HepG2 cells were pre‐treated with 12.5–50 μg mL−1 of AR ethanolic extract (HpE), which significantly increased (P < 0.05) cell viability and reduced reactive oxygen species levels. In alcohol‐stimulated mice, pre‐treatment with HpE (2.5–10 mg kg−1) enhanced the activities of superoxide dismutase, catalase, glutathione, ethanol dehydrogenase, and acetaldehyde dehydrogenase, while reducing the levels of alanine aminotransferase, aspartate aminotransferase, and triglycerides. In the anti‐melanogenesis experiment, α‐melanocyte‐stimulating hormone‐stimulated B16‐F10 mouse melanoma cells were pre‐treated with 25–100 μg mL−1 of HpE, resulting in increased tyrosinase activity and melanin content, along with a decrease in the expression levels of microphthalmia‐associated transcription factor, tyrosinase‐related proteins, and tyrosinase.CONCLUSIONHpE efficiently protects HepG2 cells from oxidative stress and safeguards the liver in mice by mitigating oxidative damage. Additionally, HpE inhibits melanin synthesis in B16‐F10 cells by suppressing tyrosinase activity. These findings suggest that H. perforatum ARs hold potential for use in the development of healthy foods, cosmetics, and pharmaceutical products aimed at antioxidative stress and anti‐melanogenesis. © 2024 Society of Chemical Industry.
Definition of Palliative Surgery in Cancer Care: A Systematic Review
Authors: Min Jolene Wong Si , Low Xinyi Casuarine , Farber Orly N. , Mack Jennifer W. , Cooper Zara , Lilley Elizabeth J. 
Published: November 2024

ABSTRACTPalliative surgery is commonly performed in cancer centers worldwide. Yet, there is little agreement on the definition of palliative surgery or its relevant outcomes. This systematic review sought to characterize the definitions of palliative surgery and outcomes for patients with cancer undergoing thoraco‐abdominal procedures. Following PRISMA guidelines, we conducted a search using PubMed, EMBASE and CINAHL databases to identify English‐language publications between August 1, 2005, and December 31, 2023 reporting palliative thoraco‐abdominal procedures for patients with cancer. Definitions of palliative surgery were coded and analyzed using an inductive approach. Outcomes were classified according to an outcome measures hierarchy. Among 92 articles met inclusion criteria and four themes emerged in how palliative surgery was defined throughout the literature: prognosis (incurable cancer diagnosis), purpose (intent to treat symptoms or improve quality of life), procedure type (specific operative interventions), or persistent disease following surgery (incomplete cytoreduction). Survival (90%) and perioperative complications/morbidity (72%) were the most commonly reported outcomes, whereas symptom relief, quality of life, and sustainability of success were infrequently reported. Definitions of palliative surgery vary across studies of patients with cancer undergoing thoracic or abdominal procedures and measured outcomes often do not align with the intent of surgery.
Chondrosarcoma of the Flat Bones: Differential Survival Between High‐Grade Lesions of the Pelvis and Scapula
Authors: Sullivan Mikaela H. , Broida Samuel E. , Cleary Emmett J. , Rose Peter S. , Houdek Matthew T. 
Published: November 2024

ABSTRACTBackgroundFlat bone chondrosarcomas have worse outcomes than extremity tumors, but there is no data directly comparing the different flat bones. The aim of this study was to examine differences in recurrence and survival between pelvic and scapular chondrosarcoma.MethodsOne hundred and sixty‐nine (42 scapula, 127 pelvic) patients with chondrosarcoma who underwent surgical resection were reviewed. High‐grade tumors were defined as lesions that were Grade 3 or dedifferentiated on surgical pathology.ResultsPatients with low‐intermediate grade lesions of the scapula were more likely to have positive margins during definitive surgical management (14% vs. 3%, OR 5, 95% CI [1.15, 22.6], p = 0.02), however, this did not translate to differences in recurrence or survival (p > 0.05). The presence of an associated soft tissue mass in low‐intermediate grade lesions was not associated with increased recurrence. Among high‐grade tumors, patients with scapular lesions had worse DSS (HR 2.99, 95% CI [1.05, 8.51], p = 0.04). One‐ and 2‐year DSS for high‐grade pelvic tumors was 75% and 57%, respectively, and 50% and 33% for scapular tumors.ConclusionSurvival for high‐grade chondrosarcoma of the flat bones is poor, particularly for those of the scapula. Despite a higher rate of positive margins for low‐intermediate grade lesions of the scapula, there was no significant difference in survival compared to low‐intermediate grade lesions of the pelvis. Additional studies with larger sample sizes are needed to further elucidate differences between these locations.
Development of a Novel Nomogram Based on Ultrasonic Radiomics for Predicting Intrauterine Pregnancy After Frozen Embryo Transfer Cycle
Authors: Yang Xin , Zheng Yu , Li Xiaohui , Wu Yuan , Fan Yao , Lv Wenzhi , Zeng Zhen , Xu Xiaoyan 
Published: November 2024

ObjectivesThis study aimed to develop a nomogram for predicting intrauterine pregnancy after an in vitro frozen embryo transfer cycle using endometrial ultrasound radiomics.MethodsA total of 211 patients who underwent ultrasound examination on the day of endometrial transformation before the frozen embryo transfer cycle were enrolled. The patients were divided into an intrauterine pregnancy group and a pregnancy failure group based on ultrasound results. Clinical characteristics and radiomic features were analyzed using univariate and multivariate logistic regression analyses. A nomogram prediction model was established based on radiomic signatures and significant clinical factors. The model's robustness was assessed in training and external validation cohorts.ResultsNine radiomic features were selected using least absolute shrinkage and selection operator (LASSO), and the radiomics score (Rad‐score) was calculated as the sum of each feature multiplied by the nonzero coefficient from LASSO. The area under the curve (AUC) of the receiver operating characteristic (ROC) curve based on the Rad‐score was 0.72, 0.65, and 0.69 in the training, validation, and combined cohorts, respectively. To improve diagnostic efficiency, the Rad‐score was further integrated with clinical factors to form a novel predictive nomogram. The results indicated that the AUC increased to 0.81, 0.67, and 0.77 in the training, validation, and combined cohorts, respectively. Decision curve analysis showed that the radiomics nomogram was clinically useful.ConclusionThe radiomics and clinical predictive nomogram can effectively predict intrauterine pregnancy after in vitro frozen embryo transfer and can be further applied in clinical strategy.
Digging for Literature on Tailoring Cultural Offers With and for Older People From Ethnic Minority Groups: A Scoping Review
Authors: Tierney Stephanie , Gorenberg Jordan , Santillo Marta , Westlake Debra , Wong Geoffrey , Husk Kerryn , Vougioukalou Sofia , Baxter Ruthanne , Dawson Shoba , Roberts Nia , Potter Caroline , Warburton Harriet , McDougall Beth , Latchem Johannah , Mahtani Kamal R 
Published: November 2024

ABSTRACTIntroductionSocial prescribing addresses non‐medical issues (e.g., loneliness, financial worries, housing problems) affecting physical and/or mental health. It involves connecting people to external support or services, including ‘cultural offers’–events, groups and activities run within or by cultural organisations. Such offers need to be acceptable and accessible to diverse populations if forming part of a social prescription.MethodsA scoping review was conducted to identify what existing literature, conducted in the United Kingdom, tells us about tailoring cultural offers for older people (aged 60+ years) from ethnic minority groups. Relevant literature was searched for on electronic databases, through Google, via a questionnaire to cultural organisations and by contacting the study's advisory group.ResultsScreening of 906 references–59 of which were read as full documents–resulted in six sources being included in the review. Some cultural activities described within them were run in traditional cultural spaces (e.g., museums, art galleries). Others were held in community centres. Data suggested that attending with others could reduce concerns about belonging. Barriers to engagement included low energy, language, poor confidence, accessing transport and unfamiliarity with a setting and/or activities. Provision of familiar food could help make people feel welcomed.ConclusionsReviewed papers showed that consulting with target groups is important to ensure that activities are inclusive and sympathetically delivered. The review also highlighted a paucity of published research on the topic; this means that cultural providers have little evidence to draw on when developing cultural offers for older people from ethnic minority groups.
Histologic Effects of Fractional Lasers and Energy‐Based Devices on Intradermally Injected Hyaluronic Acid Filler for Improving Skin Smoothness
Authors: Ge Shealinna , Chung Hye Jin , Weiss Robert A. 
Published: November 2024

ABSTRACTBackgroundPrior studies have shown that energy‐based devices (EBDs) over pre‐injected hyaluronic acid (HA) fillers do not significantly affect clinical outcomes. However, the impact of EBDs over newly FDA‐approved HA filler for improving skin smoothness is still undetermined.ObjectiveTo evaluate the immediate histologic changes after various popular EBDs are performed over pre‐injected, newly FDA‐approved intradermal HA filler.Materials and MethodsAbdominoplasty skin was injected with HA superficially. Zone 1 was used as untreated control, while the other zones treated with the 755‐nm picosecond laser with diffractive lens array, 1064‐nm picosecond and 1064‐nm Q‐switched lasers, radiofrequency with insulated microneedles, volumetric directional thermal impact ultrasound (VDTI), and thermomechanical fractional injury (TMFI) devices.ResultsHistology shows HA in the superficial to mid dermis. Treatment with fractional Q‐switched and picosecond lasers showed expected laser‐induced optical breakdown in the epidermis and dermis. RF microneedling, VDTI, and TMFI devices caused thermal damage of collagen bundles with dermal dehydration.ConclusionNo immediate morphological changes to HA were noted following device treatment. However, the effect of heat‐generating devices on the molecular integrity of HA fillers, which are composed of HA and water, remains uncertain.