bluuebunny/crossref_metadata_2025_split huggingface dataset:
iPNHOT: A knowledge-based approach for identifying protein-nucleic acid interaction hot spots
Authors: Zhu Xiaolei , Liu Ling , He Jingjing , Fang Ting , Xiong Yi , Mitchell Julie C. 
Published: May 2019

Abstract
Background

The interaction between proteins and nucleic acids plays pivotal roles in various biological processes such as transcription, translation, and gene regulation. Hot spots are a small set of residues that contribute most to the binding affinity of a protein-nucleic acid interaction. Compared to the extensive studies of the hot spots on protein-protein interfaces, the hot spot residues within protein-nucleic acids interfaces remains less well-studied, in part because mutagenesis data for protein-nucleic acids interaction are not as abundant as that for protein-protein interactions.

Results

In this study, we built a new computational model, iPNHOT, to effectively predict hot spot residues on protein-nucleic acids interfaces. One training data set and an independent test set were collected from dbAMEPNI and some recent literature, respectively. To build our model, we generated 97 different sequential and structural features and used a two-step strategy to select the relevant features. The final model was built based only on 9 features using a support vector machine (SVM). The features include three unique features such as ∆SASsa1/2, ∆SASna1/2, and esp3, which are newly proposed in this study. Based on the cross validation results, our model gave F1 score and AUC as 0.720 and 0.807 on the subset collected from ProNIT, respectively, compared to 0.407 and 0.670 of mCSM-NA, a state-of-the art model to predict the thermodynamic effects of protein-nucleic acid interaction. The iPNHOT model was further tested on the independent test set, which showed that our model outperformed other methods.

Conclusion

In this study, by collecting data from a recently published database dbAMEPNI, we proposed a new model, iPNHOT, to predict hotspots on protein-nucleic acid interfaces. The result shows that our model outperformed the existing state-of-art models. Our model is available for users through a webserver: http://zhulab.ahu.edu.cn/iPNHOT/.
Tratamento de Fratura Le fort I em paciente jovem: relato de caso
Authors: Mulinari-Santos Gabriel , Fernandes Breno dos Reis , Mendes Bruno Coelho , Queiroz Sormani Bento Fernandes de , Coléte Juliana Zorzi , Souza Francisley Ávila , Garcia Júnior Idelmo Rangel 
Published: May 2019

Em seu estudo, René Le Fort caracterizou três tipos de padrões fratura: Le Fort I, II e III. Na Le Fort I, observou um traço de fratura transverso na maxila acima do ápice dos dentes. Com o aumento da força de intensidade nos traumas, os três padrões de fratura Le Fort, são cada vez mais improváveis de acontecer de forma isolada. Com isso, este trabalho tem como objetivo apresentar um caso clínico de uma fratura do tipo Le Fort I em um paciente jovem, tratada com placas de titânio nos pilares caninos e zigomáticos da maxila.Descritores: Traumatologia; Maxila; Fixação de Fratura.ReferênciasHupp JR, Ellis III E, Myron R. Cirurgia oral e maxilo facial –Tucker. 6. Ed; 2015.Bailey BJ. Head and Neck Surgery-Otolaryngology. Philadelphia: JB Lippincott Company; 2001.Tessier P. The classic reprint: experimental study of fractures of the upper jaw. I and II. René Le Fort, MD. Plast Reconstr Surg. 1972; 50(5):497-506.Tessier P. The classic reprint: experimental study of fractures of the upper jaw. 3. René Le Fort, MD, Lille, France. Plast Reconstr Surg. 1972;50(6):600-7.Cummings CW. Otolaryngology-Head and Neck Surgery. St. Louis, USA, Mosby Year Book,1993, vol. 1Philadelphia: JB Lippincott Company; 2001.Miniti A. Bento,R.F. Butugan, O. ORL Clínica e cirúrgica. São Paulo: Atheneu; 1993.Birolini D, Utiyama E, Steinman E. Cirurgia de Emergência. São Paulo, Atheneu; 1997.Juhl JH. Crummy AB. Paul and Juhl´s Essential of Radiologic Imaging; 1996.Manson PN, Clark N, Robertson B, Slezak S, Wheatly M, Vander Kolk C et al. Subunit principles in midface fractures: the importance of sagittal buttresses, soft-tissue reductions, and sequencing treatment of segmental fractures. Plast Reconstr Surg. 1999;103(4):1287-306.
Virome and Inflammasomes, a Finely Tuned Balance with Important Consequences for the Host Health
Authors: Freer Giulia , Maggi Fabrizio , Pistello Mauro 
Published: May 2019

Background:The virome is a network of viruses normally inhabiting humans. It forms a conspicuous portion of the so-called microbiome, once generically referred to as resident flora. Indeed, viruses infecting humans without leading to clinical disease are increasingly recognized as part of the microbiome and have an impact on the development of our immune system. In addition, they activate inflammasomes, multiprotein complexes that assemble in cells and that are responsible for the downstream effects of sensing pathogens.Objective:This review aims at summarizing the evidence on the role of the virome in modulating inflammation and emphasizes evidence for Anelloviruses as useful molecular markers to monitor inflammatory processes and immune system competence.Method:We carried out a review of the literature published in the last 5 years and summarized older literature to take into account ground-breaking discoveries concerning inflammasome assembly and virome.Results:A massive amount of data recently emerging demonstrate that the microbiome closely reflects what we eat, and many other unexpected variables. Composition, location, and amount of the microbiome have an impact on innate and adaptive immune defences. Viruses making up the virome contribute to shaping the immune system. Anelloviruses, the best known of such viruses, are present in most human beings, persistently without causing apparent disease. Depending on their interplay with such viruses, inflammasomes instruct host defences to tolerate or forfeit a specific microorganism.Conclusion:The virome plays an important role in shaping human immune defences and contributes to inflammatory processes by quenching or increasing them.
Travelers’ Diarrhea: A Clinical Review
Authors: Leung Alexander K.C. , Leung Amy A.M. , Wong Alex H.C. , Hon Kam L. 
Published: May 2019

Background:
Travelers’ diarrhea is the most common travel-related malady. It affects millions of international travelers to developing countries annually and can significantly disrupt travel plans.

Objective:
To provide an update on the evaluation, diagnosis, treatment, and prevention of traveler’s diarrhea.

Methods:
A PubMed search was completed in Clinical Queries using the key term “traveler’s diarrhea”. The search strategy included meta-analyses, randomized controlled trials, clinical trials, observational studies, and reviews. The search was restricted to English literature. Patents were searched using the key term “traveler’s diarrhea” from www.freepatentsonline.com.

Results:
Between 10% and 40% of travelers develop diarrhea. The attack rate is highest for travelers from a developed country who visit a developing country. Children are at particular risk. Travelers’ diarrhea is usually acquired through ingestion of food and water contaminated by feces. Most cases are due to a bacterial pathogen, commonly, Escherichia coli, and occur within the first few days after arrival in a foreign country. Dehydration is the most common complication. Pretravel education on hygiene and on the safe selection of food items is important in minimizing episodes. For mild travelers’ diarrhea, the use of antibiotic is not recommended. The use of bismuth subsalicylate or loperamide may be considered. For moderate travelers’ diarrhea, antibiotics such as fluoroquinolones, azithromycin, and rifaximin may be used. Loperamide may be considered as monotherapy or adjunctive therapy. For severe travelers’ diarrhea, antibiotics such as azithromycin, fluoroquinolones, and rifaximin should be used. Azithromycin can be used even for the treatment of dysentery whereas fluoroquinolones and rifaximin cannot be used for such purpose. Recent patents related to the management of travelers’ diarrhea are discussed.

Conclusion:
Although travelers’ diarrhea is usually self-limited, many travelers prefer expedient relief
of diarrhea, especially when they are traveling for extended periods by air or ground. Judicious use of
an antimotility agent and antimicrobial therapy reduces the duration and severity of diarrhea.
Antiproliferative Effects of Ellagic Acid on DU145 Cells
Authors: Carota Giuseppe , Sferrazzo Giuseppe , Spampinato Mariarita , Sorrenti Valeria , Vanella Luca 
Published: May 2019

Background:Prostate Cancer (PC) represents a leading cause of tumor-related death among men in the Western world. Above all, DU145 cell line represents the most particular cells model of PC, derived from a central nervous system metastasis. In recent years, functional and healthy diet has gained a pivotal role in society, allowing the possibility to deal with cancer before its emergence or progression, profiting by anti-tumor properties of dietary phytochemicals. Among them, Ellagic Acid (EA) is found in several fruits and vegetables, whose juice demonstrated antioxidant, anti-carcinogenic and anti-fibrotic properties.Methods:DU145 prostate cancer cell line was used to determine the effects of ellagic acid on cell viability. In order to evaluate metastatic feature of DU145, VEGF-A and OPG levels by ELISA assay were assessed. Expression of β-catenin, HO-1, HO-2 and SIRT1, markers of proliferative and defense capacities, were determined by western blotting. To strengthen the study, cell transfection with siRNA β-catenin was performed.Results:In the presence of EA, the viability of DU145 cells was reduced by about 40 and 50%, respectively after the exposure to 50 and 100 μM concentrations. We also observed a reduction of both levels of VEGF-A and OPG, confirming the important role of EA in facing the metastasis development. EA treatment (50 μM) induced a significant reduction of β-catenin and SIRT1 levels and, similarly, there was a decrease of HO protein expression, more pronounced for HO-2, showing EA activity on the proliferative feature of DU145 cells. Knockdown of β-catenin by siRNA, in the presence of EA treatment, inhibited cell proliferation.Conclusion:Ellagic acid exhibits significant antiproliferative effects in ourin vitromodel of prostate cancer’s metastasis, suggesting that, the use of EA as a multitarget natural compound, may represent a possible strategy for cancer chemoprevention.
Intercambio cooperativo versus mercantilización competitiva: las políticas de movilidad académica en el MERCOSUR y la Unión Europea
Authors: Solanas Facundo 
Published: May 2019

La génesis de los procesos de internacionalización de la educación superior se vinculó estrechamente a la cooperación académica y científica entre las universidades, aunque el auge de la globalización y los procesos de mercantilización del sector transformaron en buena medida a ese intercambio en competitividad y competencia. Tanto el proceso de Bolonia como buena parte de las acciones de la Unión Europea (UE) pensadas para otros continentes, se enmarcan dentro de la estrategia para el mercado interior de servicios del Consejo Europeo de Lisboa de 2000, buscando una Europa más competitiva, donde el principal interés apuntaba a ampliar su “oferta” universitaria hacia los estudiantes de estos países. En este trabajo nos proponemos analizar cómo ha repercutido el cambio de paradigma sobre las políticas de movilidad académica en el MERCOSUR y la UE, apoyándonos en fuentes documentales y en la literatura existente en la materia.
El futuro de la educación superior en México
Authors: Acosta Silva Adrian 
Published: May 2019

Este es un ensayo sobre el futuro de la educación superior mexicana. La idea central a discutir es la naturaleza cambiante de la reflexión prospectiva no solamente de la educación superior, sino de la sociedad, la economía y la política que le sirven de escenario y contexto a la educación terciaria nacional. A partir de una revisión sobre el concepto de futuro, y de los diversos futuros que pueden ser identificados en los distintos campos de la acción pública, en el texto se exploran algunos de los discursos internacionales dominantes sobre el tema y sus posibles implicaciones para pensar el caso mexicano. Asimismo, se registran algunos de los problemas críticos de la educación superior mexicana contemporánea, y se proponen temas de lo que podría ser una política para el futuro de la educación terciaria nacional.
Motivos para la baja voluntaria definitiva de alumnos de licenciatura en instituciones de educación superior privadas. Un estudio en el Sureste de México
Authors: Barroso Tanoira Francisco Gerardo 
Published: May 2019

Las bajas voluntarias definitivas de alumnos pueden deberse a diversos factores. Sin embargo, en el caso de las instituciones de educación superior privadas, estas bajas generalmente se vinculan a problemas financieros de los alumnos o al nivel académico con el que éstos llegan desde la preparatoria. El presente estudio se realizó con participantes vinculados a cuatro instituciones de educación superior privadas en Mérida Yucatán, México, orientadas al segmento socioeconómico medio-alto. Los resultados muestran que, contrariamente a lo que comúnmente se piensa, el factor económico no fue el más importante para las bajas voluntarias definitivas de los alumnos, siendo más importantes la falta de apoyo de tutores, autoridades de la escuela y profesores (apoyo organizacional), lo que se traduce en bajo rendimiento académico, materias reprobadas, falta de orientación vocacional e insuficiente adaptación al medio, en el caso de los foráneos.Palabras clave: educación superior, desempeño del estudiante, deserción, educación privada.
La transnacionalización de la educación superior: ¿qué papel juegan los nuevos regionalismos en la difusión de estas ideas? El caso del MERCOSUR (1992-2012) en perspectiva comparada
Authors: Botto Mercedes Isabel 
Published: May 2019

El objetivo de este trabajo es contribuir a la comprensión de la reforma dela Educación Superiorde los años noventa en América Latina, analizando la influencia ejercida por los procesos de integración regional, más precisamente el MERCOSUR. ¿En qué medida ayudó a difundir y poner en marcha las ideas de transnacionalización o de internacionalización que estaban en debate a nivel global? Para responder a esta pregunta, usaremos la comparación de la experiencia del MERCOSUR con la de otros regionalismos identificando las iniciativas en torno a la movilidad de estudiantes y a la acreditación de carreras, dos iniciativas que abordan por igual todos los procesos de integración analizados. La conclusión a la que llegamos sugiere que los nuevos regionalismos avanzaron a una tercera posición: defenderse de la globalización a través de políticas de cooperación solidaria, al tiempo que buscaban conformar un espacio regional de educación superior desde donde competir globalmente. En América del Sur, sin embargo, los avances se han reducido hasta el momento, a consolidar las capacidades nacionales, promoviendo los procesos de reforma inconclusos en los países menos desarrollados y consolidar las posiciones estratégicas y dominantes de los más desarrollados en la región, sin lograr establecer un espacio regional de educación superior.
Reseña del libro La Educación Socialista en Chihuahua 1934-1940, una mirada desde la Escuela Normal del Estado, de Jesús Adolfo Trujillo Holguín
Authors: Aguirre-Lora María Esther 
Published: May 2019

En esta época donde fundamentalmente se ponderan más los artículos en revistas internacionales que las obras bien pensadas, el nacimiento de un libro es un hecho digno de celebración, tanto más si éste rescata del pasado una historia digna de contar. La educación socialista en Chihuahua, 1934-1940. Una mirada desde la Escuela Normal del Estado, de Jesús Adolfo Trujillo Holguín, es una contribución seria y destacada no sólo para la historia regional de la educación en el norte del país, sino también para la historiografía chihuahuense de nuestros días y la historiografía latinoamericana de la educación.
Comparar para construir política pública en tiempos de globalización
Authors: Guzmán-León Alicia 
Published: May 2019

El diseño de la política pública encarna la comparación y sistematización de la experiencia acumulada en lugares disímiles. Es el proceso que permite entender la experiencia ajena hasta llegar a la apropiación. La transnacionalización de las políticas demanda una metodología. Si bien existen importantes aportes sobre el método comparado desde la década de los setentas, parecería “que nos hemos embarcado en una vasta empresa comparada sin tener un método comparado” (Sartori, 2011). El halo de misterio que empapa los procedimientos y definición del método lo vuelve inaccesible. La propuesta de este artículo parte de asumir a la política pública desde una visión popperiana, donde las políticas son entendidas como teoría, es decir, no es la ingesta o suma de observaciones sino conjeturas o hipótesis puestas a prueba en la observación, con la posibilidad de ser eliminadas o mejoradas y fortalecidas en sus puntos débiles. De allí la relevancia de un marco de acción para la comparación. El objetivo de este artículo es presentar al método comparado de forma integral con el fin de facilitar su implementación en la arena académica o en la política pública. Este trabajo se divide en tres partes. La primera es la delimitación del método; la segunda sugiere un procedimiento para su implementación y finalmente; la tercera y última, establece la relevancia para el diseño y evaluación de la política pública.
Failure of Colorectal Anastomosis: Risk Factors, Prevention, Diagnosis, Therapeutic Tactics
Authors: Cherkasov M. F. , Dmitriev A. V. , Groshilin V. S. , Pereskokov S. V. , Kozyrevskiy M. A. , Urupina A. A. 
Published: May 2019

Aim. To analyse and generalize available literature data on the problem of colorectal anastomotic leakage after rectal resection. Key findings. Over the last decade, there has been an increasing trend towards sphincter-preserving operations in modern colorectal surgery. The widespread use of suturing devices of various diameters allows the formation of ultra-low anastomoses (at the level of the pelvic floor). One of the menacing complications after rectal resection is anastomotic leakage, which frequency can reach 21%. The mortality from anastomotic leakage can reach 40%. The analysed literature sources discuss a variety of risk factors, both preoperative and intraoperative, affecting the healing of the inter-intestinal anastomosis. In almost all studies, the height of the tumour and the anastomosis from the anus, preoperative radiotherapy and male sex are independent risk factors for the development of colorectal anastomotic leakage. Concerning other factors, there are conflicting opinions. The timely use of preventive measures and early diagnosis of colorectal anastomotic leakage can reduce the number and severity of postoperative complications.Conclusion. Individual consideration of risk factors and their adequate assessment in terms of possible complications are decisive in the choice of the extent of surgical intervention, which will undoubtedly improve the immediate and long-term results of the surgical treatment of colorectal cancer.
Use of Transparent Tips for Obtaining Tight Proximal Contacts in Direct Class II Composite Resin Restorations
Authors: Alonso V , Caserio M , Darriba IL 
Published: May 2019

SUMMARYThis article describes the clinical technique of using the transparent plastic tips of the LM-Contact Former system for obtaining tight proximal contacts in direct Class II composite resin restorations using noncontoured circumferential matrix bands.With this technique, the composite resin is pressed with the tip, which adapts intimately to the walls of the proximal box. Moreover, the total working time is reduced by filling the cavity, which remains after removing the transparent tip, with a bulk-fill flowable resin-based composite.
Die Analyse morphologische des adjektivs im roman ”eisprinzessin”
Authors: Marbun Rutmaida , Evianty Rina , Siahaan Jujur 
Published: May 2019

AuszugDas ZieldieserUntersuchung ist es, um Morphologische die Adjektiven herauszufinden und die Adjektive im Roman ‘Eisprinzessin’ zu beschreiben. In dieserUntersuchungwird deskriptive qualitative Methode verwendet. Die Datenquelle dieser Untersuchung ist die Adjektive im Roman ‘Eisprinzessin’ von Carla Blumberg. Die Untersuchung verwendet die Tecknik der Datensammlung, um die zu kennen, zu markieren, zu versammeln, zu analysieren, und mit dem Phänomen angesammelten die Adjektive zu verallgemeinen. Die Ergebnisse dieser Untersuchung gibt es 253 die Morphologische Adjektive, nämlich 46 die Morphologische Adjektive mit dem Präfix, 167 die Morphologische Adjektive mit dem Suffix, 5 die Morphologische Adjektive mit dem Interfiks und 35 die Morphologische Adjektive mit dem Komposition.Schlüsselwörter :Die Morphologische Des Adjektivs, Roman
Differentiation of bone marrow derived mesenchymal stem cells into male germ-like cells in co-culture with testicular cells
Authors: Malekmohamadi Nasim , Abdanipour Alireza , Ghorbanlou Mehrdad , Shokri Saeed , Shirazi Reza , Dimitriadis Eva , Nejatbakhsh Reza 
Published: May 2019

Abstract

Objective. Stem cell therapy, specifically, pre-induction of mesenchymal stem cells toward male germ-like cells may be useful in patients with azoospermia. The aim of this study was to evaluate in vitro differentiation of mouse bone marrow-derived mesenchymal stem cells (BMSCs) into male germ-like cells by indirect co-culture with testicular cells in the presence of bone morphogenetic protein 4 (BMP4).

Methods. Experimental groups included: control (mouse BMSCs), treatment group-1 (BMSCs treated with BMP4), treatment group-2 (indirect co-culture of BMSCs with mouse testicular cells in the presence of BMP4) and treatment group-3 (indirect co-culture of BMSCs with testicular cells). BMSCs-derived male germ-like cells were evaluated by the expression of Dazl, and Stra8 using RT-qPCR.

Results. Stra8 gene expression was significantly increased in the treatment group-2 and Dazl gene was significantly increased in the treatment group-1 compared to other groups. In conclusion, indirect co-culturing of BMSCs with testicular cells and BMP4 leads to the differentiation of BMSCs into male germ-like cells which express specific male germ-like genes. Testicular cells released factors that contributed to the differentiation of BMSCs into male germ progenitor cells.

Conclusion. This study suggests that mesenchymal stem cells may be differentiated into male germ-like cells and therefore, may be a novel treatment option for men with azoospermia.
Performance enhancement of split-phase induction motor by using fuzzy-based PID controller
Authors: Abdelwanis Mohamed I. , El-Sehiemy Ragab A. 
Published: May 2019

Abstract
This paper presents control and analysis of a split-phase induction motor (SPIM) to drive a centrifugal pumping system. An optimized proportional- integral and derivative (PID) controller, that is capable with a vector closed-loop split-phase induction motor control, is presented and its simulation results are discussed. The fine-tuning procedure is employed for fuzzy PID (FPID) controller parameters in order to sustain the motor speed at the predefined reference values. To assess the performance of the competitive controllers, conventional PID (CPID) and FPID, four operational indices for are suggested for measure the capability of the two controllers. These indices involve individual steady state error (ISSE) for each operating period, total steady state error (TSSE) for overall loading cycle, Individual oscillation index (IOI) and Total oscillation index (TOI), in order to measure the capability of the FPID compared with CPID. The performance of the SPIM accomplished with these performance indices is checked and tested on high and low speed levels. Pulse width modulation (PWM) based simulation studies were employed for SPIM using MATLAB/SIMULINK software. The results show that the overall performance of the SPIM operated with vector control that is tuned by FPID is enhanced compared with CPID.