bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety of Prasugrel loading in ruptured blister like aneurysm treated with a Pipeline device
Authors: Parthasarathy Rajsrinivas , Gupta Vipul , Gupta Aditya 
Published: March 2018

Objective:
Blister like aneurysm (BLA) is extremely challenging to treat; endoluminal reconstruction has emerged as the most promising treatment method. When to treat after the ictus, the timing of administration of antiplatelet and causal relationship between platelet function testing results and thrombo-embolism is unclear. We theorized that Prasugrel with a lower incidence of resistance may be a safe suitable alternative to clopidogrel in patients treated with a flow diverter (FD).

Methods:
Prospectively collected data from consecutive patients treated for a ruptured blister with an FD was reviewed. Device deployment was timed to be at 2 h following Prasugrel loading. Thrombo-embolic and hemorrhagic complications, and occlusion rates were documented.

Results:
Nine patients were included. Most were females (55%); the median age was 55 (43, 65). The median Fischer grade was 3 (2, 4). A single pipeline device was deployed in all within 24 h of admission; the median time from ictus to device deployment was 4 days (2, 30). There were no thrombo-embolic or hemorrhagic complication. Complete occlusion was noted in 89% (n = 9).

Conclusion:
Prasugrel loading timed 2 h prior to stent delivery did not increase thrombo-embolic or hemorrhagic complications. Single stent that is well apposed against the wall appears to be an effective treatment strategy to treat BLA.

Advances in knowledge:
Treatment of acutely ruptured BLA with a single pipeline device deployed at 2 h after Prasugrel loading appears to be safe.
Large–scale digitization of herbarium specimens: Development and usage of an automated, high–throughput conveyor system
Authors: Sweeney Patrick W. , Starly Binil , Morris Paul J. , Xu Yiming , Jones Aimee , Radhakrishnan Sridhar , Grassa Christopher J. , Davis Charles C. 
Published: March 2018

AbstractThe billions of specimens housed in natural science collections provide a tremendous source of under–utilized data that are useful for scientific research, conservation, commerce, and education. Digitization and mobilization of specimen data and images promises to greatly accelerate their utilization. While digitization of natural science collection specimens has been occurring for decades, the vast majority of specimens remain un–digitized. If the digitization task is to be completed in the near future, innovative, high–throughput approaches are needed. To create a dataset for the study of global change in New England, we designed and implemented an industrial–scale, conveyor–based digitization workflow for herbarium specimen sheets. The workflow is a variation of an object–to–image–to–data workflow that prioritizes imaging and the capture of storage container–level data. The workflow utilizes a novel conveyor system developed specifically for the task of imaging flattened herbarium specimens. Using our workflow, we imaged and transcribed specimen–level data for almost 350,000 specimens over a 131–week period; an additional 56 weeks was required for storage container–level data capture. Our project has demonstrated that it is possible to capture both an image of a specimen and a core database record in 35 seconds per herbarium sheet (for intervals between images of 30 minutes or less) plus some additional overhead for container–level data capture. This rate was in line with the pre–project expectations for our approach. Our throughput rates are comparable with some other similar, high–throughput approaches focused on digitizing herbarium sheets and is as much as three times faster than rates achieved with more conventional non–automated approaches used during the project. We report on challenges encountered during development and use of our system and discuss ways in which our workflow could be improved. The conveyor apparatus software, database schema, configuration files, hardware list, and conveyor schematics are available for download on GitHub.
A Gordian knot: Nomenclature and taxonomy of Heterocapsatriquetra (Peridiniales: Heterocapsaceae)
Authors: Prado Jefferson , Lendemer James , Tripp Erin , Gottschling Marc , Tillmann Urban , Kusber Wolf-Henning , Hoppenrath Mona , Elbrächter Malte 
Published: March 2018

AbstractIn the course of polyphasic taxonomic work in the dinophytes, we became aware of a fundamental misapplication of the name Glenodinium triquetrum (now represented conceptually by a species of Kryptoperidinium), when Stein assigned it to Heterocapsa. Possible solutions involve a conflict between retaining Ehrenberg’s epithet in its correct application in the interest of priority and preserving current usage of Heterocapsa. However, we do not achieve a consensus on how to disentangle this Gordian knot, underlining that this is not a regular case of taxonomic confusion. We intend to stimulate a more general discussion about best practices in such cases, balancing between the interest of nomenclatural practicability and the respectful acknowledgement of scientific work, even if it was conducted many years ago.
Areas of stigma and discrimination of mentally ill people among Internet respondents in Poland
Authors: Babicki Mateusz , Kotowicz Kamila , Piotrowski Patryk , Stramecki Filip , Kobyłko Agnieszka , Rymaszewska Joanna 
Published: March 2018

Cel pracyDane epidemiologiczne wskazują, że zaburzenia psychiczne coraz częściej występują w polskiej populacji. Świadomość dotycząca ich pochodzenia, rozpowszechnienia, objawów a także sposobów leczenia jest wciąż ograniczona. Związane z zaburzeniami psychicznymi zjawisko stygmatyzacji jest ważne ze względów moralnych a także praktycznych. Stygmatyzacja oraz dyskryminacja bardzo silnie wpływają na decyzję o podjęciu procesu terapeutycznego, a także jego przebiegu u osób chorych, w rezultacie więc na możliwość ich powrotu do normalnego funkcjonowania w życiu codziennym i pełnieniu ról społecznych.
Celem pracy było zbadanie opinii dotyczącej postrzegania osób chorych psychicznie, ich stygmatyzacji oraz dystansu wobec nich w Polsce.MetodaGrupa złożona z 1309 respondentów została przebadana autorskim kwestionariuszem rozpowszechnionym za pomocą mediów internetowych.WynikiWyniki badania wskazują na wysoki poziom stygmatyzacji pacjentów psychiatrycznych zarówno w subiektywnej ocenie ich wyglądu, intelektu, jak i poczucia wyższości nad nimi. Piętno pacjenta psychiatrycznego bardzo mocno ukazane jest również w aspekcie ekonomicznym wynikającym z niechęci respondentów do zatrudnienia, a nawet współpracy z osobami dotkniętymi schorzeniami z kręgu chorób psychicznych.WnioskiNiewątpliwie jednym z priorytetów w dziedzinie zdrowia psychicznego staje się przeciwdziałanie zjawisku stygmatyzacji oraz dyskryminacji osób dotkniętych problemem zdrowia psychicznego.
SEXUALIDADE, BRINCADEIRA E ESCOLA NO PROCESSO DE NORMALIZAÇÃO DA INFÂNCIA
Authors: Cassal Luan Carpes Barros , Chiaradia Cristiana De França 
Published: March 2018

Nos últimos anos, questões sobre infância, escola, sexualidade e gênero estão na mira de variadas proposições do Estado. Assim, este artigo propõe colocar em análise o processo de normalização das subjetividades na infância através da regulação da masturbação no espaço escolar. A construção da sexualidade das crianças, através de brincadeiras e regras, está atrelada a uma relação de gênero tradicional. Além disso, a masturbação é negada como um ato lúdico de prazer para a criança conhecer seu corpo, principalmente na escola. Porém, a tentativa de proibição de assuntos e práticas de construção do corpo e do prazer, mais do que reprimir, insiste em criar modos normalizados de existência. Assim, ao investigarmos relações entre sexualidade, infância e escola, propomos colocar em análise as proibições estabelecidas entre tais termos e a masturbação.
Using a modified Delphi methodology to gain consensus on the use of dressings in chronic wounds management
Authors: Russell David , Atkin Leanne , Betts April , Dowsett Caroline , Fatoye Francis , Gardner Sarah , Green Julie , Manu Chris , McKenzie Tracey , Meally Helena , Mitchell Louise , Mullings Julie , Odeyemi Isaac , Sharpe Andrew , Yeowell Gillian , Devlin Nancy 
Published: March 2018

Objective: Managing chronic wounds is associated with a burden to patients, caregivers, health services and society and there is a lack of clarity regarding the role of dressings in improving outcomes. This study aimed to provide understanding on a range of topics, including: the definition of chronicity in wounds, the burden of illness, clinical outcomes of reducing healing time and the impact of early interventions on clinical and economic outcomes and the role of matrix metalloproteinases (MMPs) in wound healing. Method: A systematic review of the literature was carried out on the role of dressings in diabetic foot ulcer (DFU), and venous leg ulcer (VLU) management strategies, their effectiveness, associated resource use/cost, and quality of life (QoL) impact on patients. From this evidence-base statements were written regarding chronicity in wounds, burden of illness, healing time, and the role of MMPs, early interventions and dressings. A modified Delphi methodology involving two iterations of email questionnaires followed by a face-to-face meeting was used to validate the statements, in order to arrive at a consensus for each. Clinical experts were selected, representing nurses, surgeons, podiatrists, academics, and policy experts. Results: In the first round, 38/47 statements reached or exceeded the consensus threshold of 80% and none were rejected. According to the protocol, any statement not confirmed or rejected had to be modified using the comments from participants and resubmitted. In the second round, 5/9 remaining statements were confirmed and none rejected, leaving 4 to discuss at the meeting. All final statements were confirmed with at least 80% consensus. Conclusion: This modified Delphi panel sought to gain clarity from clinical experts surrounding the use of dressings in the management of chronic wounds. A full consensus statement was developed to help clinicians and policy makers improve the management of patients with these conditions.
Flexible intramedullary nailing versus nonoperative treatment for paediatric displaced midshaft clavicle fractures
Authors: Mukhtar I. A. , Yaghmour K. M. , Ahmed A. F. , Ibrahim T. 
Published: March 2018

Purpose The treatment of displaced midshaft clavicle fractures in children remains controversial. The purpose of our study was to compare the outcome of displaced midshaft clavicle fractures in children who were managed operatively by flexible intramedullary nailing (FIN) with nonoperative treatment. Methods A prospective review of 31 children (mean age 10.5 years) with displaced midshaft clavicle fractures treated either by FIN or nonoperatively and with at least a six-month follow-up was undertaken. In all, 24 children underwent FIN and seven underwent nonoperative treatment. The patient outcomes included the Constant-Murley score, Customer Satisfaction Questionnaire (CSQ-8), numeric pain rating scale, time to union and time to return to activity. Surgical complications were recorded. Results The two groups were comparable with regards to age, gender and mechanism of injury. At six months of follow-up, the Constant-Murley (97.8 versus 94.7, p < 0.001) and CSQ-8 (29.1 versus 19.1, p < 0.001) scores were higher in the FIN group. Time to union and return to activity were significantly shorter in the FIN group (7.3 and 9.2 weeks versus 10.4 and 16.6 weeks respectively, p < 0.01). The only surgical complication was a FIN exchange for skin irritation due to nail prominence. Conclusion FIN is a minimally invasive procedure for children with displaced midshaft clavicle fractures associated with shorter time to union, quicker return to activity and higher Constant-Murley and CSQ-8 scores when compared with nonoperative treatment. However, the difference in Constant-Murley scores was not clinically significant. Furthermore, the advantages of FIN are at the expense of an increased complication rate of 12.5% (upper 95% confidence interval 33.3%). Level of Evidence Therapeutic, II
Western nightmares: Manifest Destiny and the representation of genocide in weird fiction
Authors: Manea Dragoş 
Published: March 2018

Abstract
This article focuses on the representation of genocide in Manifest Destiny (Dingess 2013–present), a comic book series loosely based on the 1804–06 Lewis and Clark expedition, replete with fantastical creatures, strange habitats and superhuman acts of heroism. The graphic narrative, published by Skybound/Image Comics, with art by Matthew Roberts and Owen Gieni, tells the story of the Corps of Discovery as they explore a quasi-mythical uncharted land, on the orders of President Jefferson. Their mission is to map and tame the newly purchased Louisiana Territory and prepare it for American settlers by killing the myriad monsters that still populate the strange land (including buffalotaurs, gigantic frogs, sentient anthropomorphic birds and a plant that infects humans and animals, turning their insides to plant-like material and robbing them of their will). By analysing it as a work of weird fiction, I explore how the presence of monstrous creatures creates a number of effects, from the horrific to the unsettling, outlines the killers’ awareness of the moral ambiguity of their actions and encourages readers’ identification with genocide perpetrators. The series, I argue, is grounded upon a formal realism that makes it highly effective in depicting scenes of weird fiction, whose defamiliarizing effect can rely on combining conventional, highly realistic settings, characters or tableaux with strange elements that pervert and yet do not render them unrecognizable. In conversation with critics and writers such as Ian Dawe, Darja Malcolm-Clarke, Simon Spiegel and Ann and Jeff VanderMeer, I attempt to explore the formal strategies adopted by the series in order to create scenes of weird fiction and their ethical implications.
Stress and ways of coping among first year nursing students: A Turkish perspective
Authors: Bektaş Hicran , Terkes Nurten , Özer Zeynep 
Published: March 2018

Aim: The aim of this descriptive study was to assess stress and ways of coping among first year nursing students.Methods: The sample consisted of 90 nursing students from baccalaureate degree programs at a university in Turkey. The research tool consisted of demographic questions, the Pagana Clinical StressQuestionnaire (CSQ) and the Ways of Coping Questionnaire (WCQ). The data collection form was performed at the end of the first clinical practice day and the re-test was performed at the end of the last clinical practice day.Results: The average age of the population was 19.72±1.32, 78.9% of the students were female. In the research, average point of the students’ CSQ and WCQ were calculated as 50.50±9.36 and 71.06±13.64 before the clinical practice, 52.07±9.87 and 77.63±17.03 after the clinical practice respectivelyand it was found that nursing students had significantly higher stress in their clinical practices.Conclusions: Nursing students experience varying degrees of stress across clinical practices and they consistently report that their clinical experiences are stressful.
Association analysis of calpain 10 gene variants/haplotypes with gestational diabetes mellitus among Mexican women
Authors: Castro-Martí­nez Anna Gabriela , Sánchez-Corona José , Vázquez-Vargas Adriana Patricia , Garcí­a-Zapién Alejandra Guadalupe , López-Quintero Andres , Villalpando-Velazco Héctor Javier , Flores-Martí­nez Silvia Esperanza 
Published: March 2018

Gestational diabetes mellitus (GDM) is a metabolically complex disease with major genetic determinants. GDM has been associated with insulin resistance and dysfunction of pancreatic beta cells, so the GDM candidate genes are those that encode proteins modulating the function and secretion of insulin, such as that for calpain 10 (CAPN10). This study aimed to assess whether single nucleotide polymorphism (SNP)-43, SNP-44, SNP-63, and the indel-19 variant, and specific haplotypes of the CAPN10 gene were associated with gestational diabetes mellitus. We studied 116 patients with gestational diabetes mellitus and 83 women with normal glucose tolerance. Measurements of anthropometric and biochemical parameters were performed. SNP-43, SNP-44, and SNP-63 were identified by polymerase chain reaction (PCR)-restriction fragment length polymorphisms, while the indel-19 variant was detected by TaqMan qPCR assays. The allele, genotype, and haplotype frequencies of the four variants did not differ significantly between women with gestational diabetes mellitus and controls. However, in women with gestational diabetes mellitus, glucose levels were significantly higher bearing the 3R/3R genotype than in carriers of the 3R/2R genotype of the indel-19 variant (p = 0.006). In conclusion, the 3R/3R genotype of the indel-19 variant of the CAPN-10 gene influenced increased glucose levels in these Mexican women with gestational diabetes mellitus.
TGFÎ²1-mediated expression and alternative splicing of Fibronectin Extra Domain A in human podocyte culture
Authors: Madne Tarunkumar Hemraj , Dockrell Mark Edward Carl 
Published: March 2018

Alternative splicing is a fundamental phenomenon to build protein diversity in health and diseases. Extra Domain A+ Fibronectin (EDA+Fn) is an alternatively spliced form of fibronectin protein present in the extra cellular matrix (ECM) in renal fibrosis. Podocytes are spectacular cell type and play a key role in filtration and synthesise ECM proteins in renal physiology and pathology. TGFÎ²1 is a strong stimulator of ECM proteins in renal injury. In this study, we have investigated alternative splicing of EDA+ Fn in human podocytes in response to TGFÎ²1. We have performed western blotting and immunofluorescence to characterise the expression of the EDA+Fn protein, real-time PCR for RNA expression and RT-PCR to look for alternative splicing of EDA+Fn in conditionally immortalised human podocytes culture.We used TGFÎ²1 as a stimulator and SB431542 and SRPIN340 for inhibitory studies. In this work, for the first time we have demonstrated in human podocytes culture EDA+Fn is expressed in the basal condition and TGFÎ²1 2.5ng/ml induced the Fn mRNA and EDA+Fn protein expression demonstrated by real-time PCR, western blotting and immunofluorescence. TGFÎ²1 2.5ng/ml induced the alternative splicing of EDA+Fn shown by conventional RT-PCR. Studies with ALK5 inhibitor SB431542 and SRPIN340 show that TGFÎ²1 induced alternative splicing of EDA+Fn was by the ALK5 receptor and the SR proteins. In human podocytes culture, alternative splicing of EDA+Fn occurs at basal conditions and TGFÎ²1 further induced the alternative splicing of EDA+Fn via ALK5 receptor activation and SR proteins. This is the first evidence of basal and TGFÎ²1 mediated alternative splicing of EDA+Fn in human podocytes culture.
Evaluation of anticancer, antioxidant activity and phenolic compounds of Artemisia absinthium L. Extract
Authors: Koyuncu Ismail 
Published: March 2018

In the treatment of cancer, which remains a fatal disease, increasingly successful treatment rates of alternative therapies using the power of plants have directed the scientific world towards natural plant resources. This study aimed to examine the anti-cancer and antioxidant properties and identify the phenolic content of the methanolic extract obtained from Artemisia absinthium L. (AR) species, which is used as folk-medicine. The antioxidant activity of the extract was investigated using total phenolics, flavonoids, ABTS and CUPRAC methods. Phenolic component analysis of the plant extract was performed by LC-MS/MS. The anti-cancer property of AR extract was investigated on human colon (DLD-1), endometrium (ECC-1) cancer cells and embryonic kidney (HEK-293) cells. Cytotoxic effects were defined with MTT, apoptotic activity with DNA fragmentation ELISA and AO/EB fluorescent staining, the genotoxic effect with the comet assay and the intracellular oxidative status with TAS and TOS methods. As a result of the study, it was determined that AR extract showed an antioxidant effect, and as a result of the content analysis made with LC-MS/MS, phenolic components were determined, the most abundant being chlorogenic acid, followed by quinic acid, cinnamic acid, rhoifolin and malic acide. AR extract showed cytotoxic activity on DLD-1 and ECC-1 cancer cells, while the cytotoxic effect on HEK-293 cells was determined to be low. It was determined that by increasing the intracellular amount of free radicals on cancer cells, this led to DNA damage, which consequently led to apoptosis of the cancer cells.
The Molecular Chaperone HSP90 Promotes Notch Signaling in the Germline ofCaenorhabditis elegans
Authors: Lissemore James L , Connors Elyse , Liu Ying , Qiao Li , Yang Bing , Edgley Mark L , Flibotte Stephane , Taylor Jon , Au Vinci , Moerman Donald G , Maine Eleanor M 
Published: March 2018

AbstractIn a genetic screen to identify genes that promote GLP-1/Notch signaling in Caenorhabditis elegans germline stem cells, we found a single mutation, om40, defining a gene called ego-3. ego-3(om40) causes several defects in the soma and the germline, including paralysis during larval development, sterility, delayed proliferation of germline stem cells, and ectopic germline stem cell proliferation. Whole genome sequencing identified om40 as an allele of hsp-90, previously known as daf-21, which encodes the C. elegans ortholog of the cytosolic form of HSP90. This protein is a molecular chaperone with a central position in the protein homeostasis network, which is responsible for proper folding, structural maintenance, and degradation of proteins. In addition to its essential role in cellular function, HSP90 plays an important role in stem cell maintenance and renewal. Complementation analysis using a deletion allele of hsp-90 confirmed that ego-3 is the same gene. hsp-90(om40) is an I→N conservative missense mutation of a highly conserved residue in the middle domain of HSP-90. RNA interference-mediated knockdown of hsp-90 expression partially phenocopied hsp-90(om40), confirming the loss-of-function nature of hsp-90(om40). Furthermore, reduced HSP-90 activity enhanced the effect of reduced function of both the GLP-1 receptor and the downstream LAG-1 transcription factor. Taken together, our results provide the first experimental evidence of an essential role for HSP90 in Notch signaling in development.