bluuebunny/crossref_metadata_2025_split huggingface dataset:
Smartcard: an integrated approach for contaminant monitoring, from field to laboratory
Authors: Geballa-Koukoula Ariadni , Willemsen Linda , Beij Erik , van Hoof Richard , Elferink Alexander , Geballa-Koukoulas Khalil , Peters Jeroen , Blokland Marco H. , Salentijn Gert IJ. 
Published: November 2024

AbstractEffective food safety monitoring requires a multi-step approach from farm to fork, involving different methods, ranging from convenient screening devices to sophisticated laboratory confirmatory testing. However, sample transportation to routine laboratories is time-consuming and expensive. Simplified on-site sampling followed by laboratory analysis offers a potential solution. Dried blood spot (DBS) cards ensure stability and ease of sample transportation and are used in clinical testing. However, the applicability of such an approach could be broader and include the storage of dried extract from more complex (solid) matrices. Therefore, a simplified approach is presented here, using DBS cards for on-site sampling and subsequent laboratory confirmation for food contaminants. To achieve this, an analytical tool (Smartcard) was designed using 3D-printing technology. As a proof of concept, the approach was applied to detect the pesticide fipronil, which is widely used in ornamental flower production to limit pests and on poultry farms. The Smartcard can securely store the sample extracts on a DBS card (dried extract spot (DExS) card), incorporate the lateral flow immunoassay (LFIA) and immediately provide an estimate of contamination levels. After simplified in-syringe extraction of the sample, the LFIA allows direct screening of fipronil (half maximum inhibitory concentration of 6.5 µg/l with calibration standards), and the same sample extract can be directly applied to the DExS card for storage and transport to the laboratory, where analyte re-extraction and instrumental analysis is performed using ultra high-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detecting fipronil down to 0.8 µg/kg.
Graphical Abstract
Surface monitoring of L. monocytogenes by real-time fluorescence and colorimetric LAMP
Authors: Abalo Maruxa , Lamas Alexandre , Teixeira Carla , Prado Marta , Garrido-Maestu Alejandro 
Published: November 2024

Abstract
Listeria monocytogenes is a major foodborne pathogen affecting developing, and developed countries. The analysis of food contact surfaces in food industries is key for better controlling this pathogen. The current study focused on the development, optimization, and evaluation of a rapid and simple method for the detection of L. monocytogenes on stainless steel surfaces, suitable for decentralized setups, taking advantage of Loop-mediated isothermal amplification (LAMP). This was accomplished using a general pre-enrichment broth (TSB), with a simple DNA extraction based on a chelating resin, and final isothermal amplification. Two different detection strategies were tested, real-time fluorescence and naked-eye colorimetric, which were evaluated after 5, 7, and 24 h of pre-enrichment. Regardless the detection chemistry selected, after 5–7 h of pre-enrichment, 103–104 CFU/cm2 were needed to obtain a positive result, while after 24 h, it was possible to detect concentrations below 10 CFU/cm2. Within each given time, all the performance parameters calculated, relative sensitivity, specificity, and accuracy, reached values higher than 80–90%; likewise, a Cohen’s k of concordance with a culture-based approach higher than 0.8. Overall, the most sensitive assay can be performed in roughly 25 h. This time-to-result outperforms commercial kits with the added value of specifically detecting L. monocytogenes instead of Listeria spp.

Key points
• Real-time fluorescence and naked-eye colorimetric, were compared for the novel assay.
• An LOD50 of 3.4 CFU/cm2 and 4.2 CFU/cm2 was calculated for the two assays.
• Three pre-enrichment times were compared providing 24 h better results.
EANM expert opinion: How can lessons from radiobiology be applied to the design of clinical trials? Part I: back to the basics of absorbed dose–response and threshold absorbed doses
Authors: Pouget Jean-Pierre , Gabina Pablo Minguez , Herrmann Ken , Deandreis Desirée , Konijnenberg Mark , Taieb David , van Leeuwen Fijs W. B. , Kurth Jens , Eberlein Uta , Lassmann Michael , Lückerath Katharina ,   For the EANM Radiobiology Working Group
Published: November 2024

Abstract

Purpose
This study by the EANM radiobiology working group aims to analyze the efficacy and toxicity of targeted radionuclide therapy (TRT) using radiopharmaceuticals approved by the EMA and FDA for neuroendocrine tumors and prostate cancer. It seeks to understand the correlation between physical parameters such as absorbed dose and TRT outcomes, alongside other biological factors.

Methods
We reviewed clinical studies on TRT, focusing on the relationship between physical parameters and treatment outcomes, and applying basic radiobiological principles to radiopharmaceutical therapy to identify key factors affecting therapeutic success.

Results
The analysis revealed that mean absorbed dose alone is insufficient to predict treatment response or toxicity. For absorbed doses below a certain threshold, outcomes are unpredictable, while doses above this threshold improve the likelihood of biological responses. However, even at higher absorbed doses, response plateaus indicate the need for additional parameters to explain outcome variability, including heterogeneity in target expression, anatomical disease location, (epi)genetics, DNA repair capacity, and the tumor microenvironment, aspects that will be discussed in Part II of this analysis.

Conclusion
Understanding radiobiology is crucial for optimizing TRT. More dosimetric data is needed to refine treatment protocols. While absorbed dose is critical, it alone does not determine TRT outcomes. Future research should integrate biological parameters with physical dosimetry to enhance efficacy and minimize toxicity.
Towards the clinical translation of a silver sulfide nanoparticle contrast agent: large scale production with a highly parallelized microfluidic chip
Authors: Mossburg Katherine J. , Shepherd Sarah J. , Barragan Diego , O Nathaniel H. , Berkow Emily K. , Maidment Portia S. N. , Rosario Berrios Derick N. , Hsu Jessica C. , Siedlik Michael J. , Yadavali Sagar , Mitchell Michael J. , Issadore David , Cormode David P. 
Published: November 2024

Abstract

Purpose
Ultrasmall silver sulfide nanoparticles (Ag2S-NP) have been identified as promising contrast agents for a number of modalities and in particular for dual-energy mammography. These Ag2S-NP have demonstrated marked advantages over clinically available agents with the ability to generate higher contrast with high biocompatibility. However, current synthesis methods for inorganic nanoparticles are low-throughput and highly time-intensive, limiting the possibility of large animal studies or eventual clinical use of this potential imaging agent.

Methods
We herein report the use of a scalable silicon microfluidic system (SSMS) for the large-scale synthesis of Ag2S-NP. Ag2S-NP produced using this system were compared to bulk synthesis and a commercially available microfluidic device through characterization, contrast generation, in vivo imaging, and clearance profiles.

Results
Using SSMS chips with 1 channel, 10 parallelized channels, and 256 parallelized channels, we determined that the Ag2S-NP produced were of similar quality as measured by core size, concentration, UV–visible spectrometry, and in vitro contrast generation. Moreover, by combining parallelized chips with increasing reagent concentration, we were able to increase output by an overall factor of 5,100. We also found that in vivo imaging contrast generation was consistent across synthesis methods and confirmed renal clearance of the ultrasmall nanoparticles. Finally, we found best-in-class clearance of the Ag2S-NP occurred within 24 h.

Conclusions
These studies have identified a promising method for the large-scale production of Ag2S-NP, paving the way for eventual clinical translation.
Vector-borne disease outbreak control via instant releases
Authors: Almeida Luis , Bellver-Arnau Jesús , Privat Yannick , Rebelo Carlota 
Published: November 2024

AbstractThis paper is devoted to the study of optimal release strategies to control vector-borne diseases, such as dengue, Zika, chikungunya and malaria. Two techniques are considered: the sterile insect one (SIT), which consists in releasing sterilized males among wild vectors in order to perturb their reproduction, and the Wolbachia one (presently used mainly for mosquitoes), which consists in releasing vectors, that are infected with a bacterium limiting their vectorial capacity, in order to replace the wild population by one with reduced vectorial capacity. In each case, the time dynamics of the vector population is modeled by a system of ordinary differential equations in which the releases are represented by linear combinations of Dirac measures with positive coefficients determining their intensity. We introduce optimal control problems that we solve numerically using ad-hoc algorithms, based on writing first-order optimality conditions characterizing the best combination of Dirac measures. We then discuss the results obtained, focusing in particular on the complexity and efficiency of optimal controls and comparing the strategies obtained. Mathematical modeling can help testing a great number of scenarios that are potentially interesting in future interventions (even those that are orthogonal to the present strategies) but that would be hard, costly or even impossible to test in the field in present conditions.
Evidence synthesis of medical cannabis research: current challenges and opportunities
Authors: Senator Ben , Pardal Mafalda , Vandam Liesbeth 
Published: November 2024

Abstract
As a wide group of medicines, the effectiveness and safety of ‘medical cannabis’ products is likely to vary in relation to product-specific dimensions such as potency, dosage, route of administration, and cannabinoid composition. Systematic reviews can perform a crucial role in analysing and synthesising the outcomes of medical cannabis interventions found in empirical research. We analysed 23 contemporary systematic reviews on the effectiveness and safety of medical cannabis to discern the extent to which this body of work aimed to capture, and ultimately captured, the differing outcomes of medical cannabis products by product-specific dimensions of treatment. We further highlighted the methodological reasons given by authors for an inability to describe this granular level of information. We found that a minority of systematic reviews explicitly aimed to perform a subgroup analysis to determine differences in treatment outcomes by product-specific dimensions of medical cannabis, with even fewer subsequently doing so. Authors’ stated reasons for this concerned either overly large or overly small levels of variation in the characteristics, compositions, and administrations of medical cannabis products used, rendering subgroup analyses methodologically inappropriate or inapplicable. Furthering systematic reviews’ abilities to capture granular information on medical cannabis treatment outcomes in relation to product-specific dimensions of treatments will require further standardisation of treatments in empirical studies.
Results of a German nationwide survey on perioperative cardiac management in vascular surgery
Authors: Dovzhanskiy Dmitriy I. , Bischoff Moritz S. , Passek Karola , Böckler Dittmar 
Published: November 2024

Abstract
Because of the lack of specific recommendations concerning cardiac risk stratification before vascular surgery, appropriate decisions remain individual. The aim of the present study was to evaluate the perioperative cardiac management in vascular surgery in Germany.

Methods
This article is based on a survey from 2018 of heads of German vascular surgical departments or units regarding their experience with perioperative cardiac management. The questionnaire asked about the experience with preoperative cardiac evaluation and its extension, awareness of perioperative myocardial ischemia, the art of postoperative monitoring and the routine use of the best medical treatment.

Results
In total, 62% of responders agreed that perioperative myocardial ischemia is a relevant postoperative problem in their clinic after open abdominal aortic surgery, while 47% stated the same after vascular surgery (VS) like carotid endarterectomy, peripheral arterial surgery or EVAR. Preoperative cardiological evaluations are performed routinely by 87% of responders before open abdominal aortic surgery and by 42% before VS. Preoperative cardiac evaluation included cardiac echography in 92% and stress diagnostics (stress echography, stress ECG) in 38%. Routine preoperative cardiac catheterisation is performed in 4% before OAS and only 0.5% before VS. In addition, 79% of participants initiate acetylsalicylic acid routinely and 68% use statins preoperatively. The serum troponin diagnostic test in asymptomatic patients was routinely applied by 19% of responders after OAS and by 6% after VS.

Conclusion
Perioperative myocardial ischemia is considered a relevant problem, primarily after aortic surgery. The preoperative cardiac stress diagnostics among vascular surgeons does not seem to be sufficiently widespread. The preoperative initiation of acetylsalicylic acid and statins is not routine in 30% of hospitals.
Intracellular cAMP signaling-induced Ca2+ influx mediated by calcium homeostasis modulator 1 (CALHM1) in human odontoblasts
Authors: Kimura Maki , Nomura Sachie , Ouchi Takehito , Kurashima Ryuya , Nakano Rei , Sekiya Hinako , Kuroda Hidetaka , Kono Kyosuke , Shibukawa Yoshiyuki 
Published: November 2024

Abstract
In odontoblasts, intracellular Ca2+ signaling plays key roles in reactionary dentin formation and generation of dentinal pain. Odontoblasts also express several Gs protein-coupled receptors that promote production of cyclic AMP (cAMP). However, the crosstalk between intracellular cAMP and Ca2+ signaling, as well as the role of cAMP in the cellular functions of odontoblasts, remains unclear. In this study, we measured intracellular cAMP levels and intracellular free Ca2+ concentration ([Ca2+]i). We also investigated the effect of intracellular cAMP on mineralization by the odontoblasts. In the presence of extracellular Ca2+, the application of forskolin (adenylyl cyclase activator) or isoproterenol (Gs protein-coupled beta-2 adrenergic receptor agonist) increased intracellular cAMP levels and [Ca2+]i in odontoblasts. The [Ca2+]i increases could not be observed by removing extracellular Ca2+, indicating that cAMP is capable to activate Ca2+ entry. Forskolin-induced [Ca2+]i increase was inhibited by a protein kinase A inhibitor in odontoblasts. The [Ca2+]i increase was sensitive to Gd3+, 2APB, or Zn2+ but not verapamil, ML218, or La3+. In immunofluorescence analyses, odontoblasts were immunopositive for calcium homeostasis modulator 1 (CALHM1), which was found close to ionotropic ATP receptor subtype, P2X3 receptors. When CALHM1 was knocked down, forskolin-induced [Ca2+]i increase was suppressed. Alizarin red and von Kossa staining showed that forskolin decreased mineralization. These findings suggest that activation of adenylyl cyclase elicited increases in the intracellular cAMP level and Ca2+ influx via protein kinase A activation in odontoblasts. Subsequent cAMP-dependent Ca2+ influx was mediated by CALHM1 in odontoblasts. In addition, the intracellular cAMP signaling pathway in odontoblasts negatively mediated dentinogenesis.
Investigating the effect of cognitive load on the intentionality bias
Authors: Eisenkoeck A. E. , de Fockert J. W. , Moore J. W. 
Published: November 2024

AbstractAccording to Rosset’s dual-process model of intention attribution, our judgements of intentionality can be guided either by an automatic process leading to intentional explanations of behaviour or by a higher-level and cognitively more demanding process enabling unintentional explanations of behaviour. Based on this model, under conditions of compromised cognitive capacity, individuals should judge more behaviour to be intentional rather than unintentional. This prediction was tested in one lab-based experiment and one online experiment. Specifically, we investigated whether increased working memory load would lead to higher intentionality endorsement of ambiguous action when controlling for individual differences in working memory. Results of both experiments indicated no effect of working memory load on intentionality endorsement. The implications of these results for the dual-process model of intention attribution are discussed.
Interactions involved in the adsorption of ethylene glycol and 2-hydroxyethoxide on the Au(111) surface: a Density Functional Theory study
Authors: Avelar Joana , Hernández-Esparza Raymundo , Garza Jorge , Vargas Rubicelia 
Published: November 2024

AbstractContext: The monolayers of ethylene glycol and 2-hydroxyethoxide on gold surfaces have been used in hybrid materials as biosensors. In this article, the adsorption of ethylene glycol and 2-hydroxyethoxide on the Au(111) surface was analyzed. For the first system, ethylene glycol on Au(111), there are Au$$\cdot \cdot \cdot $$

·
·
·

O and Au$$\cdot \cdot \cdot $$

·
·
·

H interactions. To the best of our knowledge, the Au$$\cdot \cdot \cdot $$

·
·
·

H interaction has been overlooked until now. However, in this work, there is strong evidence that this interaction is important to stabilize the system. For the second system, the atomic interactions mentioned previously are also predicted, although there is an additional interaction between 2-hydroxyethoxide molecules. Such an interaction induces the link -O-H-O-, with high values of the electron density at the critical points of the corresponding bond path of the O-H interaction. These links suggest the forming of ethylene glycol chains. Methods: The calculations were performed using two exchange-correlation functionals: BEEF-vdW and C09$$_{x}$$

x

-vdW; both functionals incorporate dispersion effects within the Kohn-Sham approach in Density Functional Theory as implemented in GPAW code and ASE computational packages. The contacts between the molecules considered in this article and the Au(111) surface were analyzed through the Quantum Theory of Atoms in Molecules implemented in GPUAM code.
Combined fractional CO2 laser with topical tioconazole versus Q-switched Nd-YAG laser in the treatment of onychomycosis; a randomized comparative trial
Authors: Sayed Khadiga , Khaled Manar , Gad Abdallah , Elbendary Amira 
Published: November 2024

Abstract
Treatment of onychomycosis includes topical and systemic agents. However, prolonged use of oral treatment could cause adverse effects and topical antifungal agents have limited penetration. To compare the clinical efficacy and the safety of fractional CO2 laser combined with topical tioconazole nail solution versus Q-switched 1064 Nd: YAG laser in the treatment of fingernail onychomycosis. This randomized comparative clinical trial was conducted on 13 patients (47 nails) with fingernail onychomycosis. Patients were randomized to receive either fractional CO2 laser combined with topical tioconazole or Q-Switched Nd: YAG 1064 nm laser every 2 weeks for 3 months followed by a 1-month follow up assessment. Onychomycosis severity index (OSI) score, Dermatology life Quality Index (DLQI) score, patient satisfaction score, dermoscopic evaluation and KOH examination were used for assessment of improvement. OSI showed improvement after treatment in both arms (from 16.17 to 10.92 in fractional CO2 arm (
p

= 0.026) and 23.13 to 22.43 (
p

= 0.92)). When comparing both groups OSI score significantly reduced in the fractional CO2 laser combined with tioconazole more than the Q-switched laser group (p = 0.002). The mean DLQI score significantly improved in both groups but no statistically significant difference between the two groups. Significant improvement in patient satisfaction score was noted in both groups. Mycological cure using KOH examination was detected in both groups (44.4% in the CO2 group and 56.5% in the Q-switched group) with no significant difference (P value < 0.05). Ruin pattern keratosis found to be the most dermoscopic pattern to be associated with poor OSI score improvement. Fractional CO2 laser combined with topical tioconazole is more efficient in treatment of onychomycosis than Q-Switched Nd: YAG 1064 nm laser group as regards clinical improvement but both have comparable effect on mycological cure. Their use as adjuvant treatment rather than alone is recommended to ensure mycological cure in onychomycosis.
Extended formulations for binary optimal control problems
Authors: Buchheim Christoph 
Published: November 2024

AbstractExtended formulations are an important tool in polyhedral combinatorics. Many combinatorial optimization problems require an exponential number of inequalities when modeled as a linear program in the natural space of variables. However, by adding artificial variables, one can often find a small linear formulation, i.e., one containing a polynomial number of variables and constraints, such that the projection to the original space of variables yields a perfect linear formulation. Motivated by binary optimal control problems with switching constraints, we show that a similar approach can be useful also for optimization problems in function space, in order to model the closed convex hull of feasible controls in a compact way. More specifically, we present small extended formulations for switches with bounded variation and for dwell-time constraints. For general linear switching point constraints, we devise an extended model linearizing the problem, but show that a small extended formulation that is compatible with discretization cannot exist unless P = NP.
Geodesic loops on tetrahedra in spaces of constant sectional curvature
Authors: Borisenko A. , Miquel V. 
Published: November 2024

Abstract
Geodesic loops on tetrahedra were studied only for the Euclidean space and it was known that there are no simple geodesic loops on regular tetrahedra. Here we prove that: 1) In the spherical space, there are no simple geodesic loops on tetrahedra with internal angles

$$\pi/3 < a_i<\pi/2$$

π
/
3
<

a
i

<
π
/
2

or regular tetrahedra with

$$a_i=\pi/2$$

a
i

=
π
/
2

, and there are three simple geodesic loops for each vertex of a tetrahedra with

$$a_i > \pi/2$$

a
i

>
π
/
2

and the lengths of the edges

$$a_i>\pi/2$$

a
i

>
π
/
2

. 2) We obtain also a new theorem on simple closed geodesics: If the angles

$$a_i$$

a
i

of the faces of a tetraedron satisfy

$$\pi/3 < a_i<\pi/2$$

π
/
3
<

a
i

<
π
/
2

and all faces of the tetrahedron are congruent, then there exist at least

$$3$$

3

simple closed geodesics.
3) In the hyperbolic space, for every regular tetrahedron

$$T$$

T

and every pair of coprime numbers

$$(p,q)$$

(
p
,
q
)

, there is one simple geodesic loop of type

$$(p,q)$$

(
p
,
q
)

through every vertex of

$$T$$

T

.
The geodesic loops that we have found on the tetrahedra in the hyperbolic space are also quasi-geodesics.