bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNF213 gene mutation of circulating tumor DNA in early diagnosis of NSCLC using targeted next-generation sequencing
Authors: Jiang Ning , Zhao Shukang , Li Peichao , Liu Yu , Shi Hubo , Zhang Chengke , Wang Yunshan , Zhou Chengjun , Zhang Wenhao , Peng Chuanliang , Zhang Weiquan , Hao Yingtao , Zhao Yunpeng , Sun Qifeng , Zhao Xiaogang 
Published: July 2019

Abstract
Introduction

To distinguish early stage lung cancer from benign disease of the lung nodules, especially the lesions with ground-glass opacity (GGO) or ground-glass nodule (GGN), we assessed gene mutations of the ctDNA in peripheral blood by using targeted next-generation sequencing (NGS).

Methods

Single lung nodule patients without mediastinal lymph nodes or symptoms hardly diagnosed by chest CT and biomarker of lung cancer were enrolled. All patients received minimally invasive surgery but refused preoperative biopsy. Gene mutations of pre-operative blood samples were detected by targeted NGS. Mutations with statistical differences were screened in lung cancer and benign disease grouped by postoperative pathology. Gene expression was determined by immunohistochemistry. Highly expressed genes were selected as biomarkers to verify the mutations in peripheral blood.

Results

In training set, RNF213, KMT2D, CSMD3 and LRP1B genes mutated more frequently in early stage lung cancer (25cases) than benign nodules (18cases) (P<0.05). High expressions of RNF213 gene in lung cancers and low expressions in benign diseases were evaluated by immunohistochemistry. RNF213 gene mutated in 25% lung cancer samples in the validation set of 28 samples and showed high specificity (100%) and low sensibility (25.9%). In GGO and GGN patients, RNF213 mutated more frequently in early stage lung cancer compared to benign diseases (P<0.05).

Conclusions

RNF213 gene mutation was observed more frequently in early stage lung cancer, but rather than benign nodules. Mutation of RNF213 gene in peripheral blood may be a high specificity biomarker and valuable for early diagnosis of lung cancer.
Prevalence of Shiga toxin-producing Escherichia coli isolated from chicken meat in west of Iran
Authors: zarei omid , Shokoohizadeh Leili , Hossainpour Hadi , Alikhani Mohammad Yousef 
Published: July 2019

Abstract
Objective: Shiga toxin producing Escherichia coli (STEC) has known as a crucial zoonotic food borne pathogen. A total of 257 row chicken meat samples were collected from different markets in Hamadan city from January 2016 to May 2017. Samples were cultured on selective and differential culture media, and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by disk diffusion method. The genetic relatedness of STEC isolates were analyzed by ERIC-PCR.

Results: Totally, 93(36%) of isolates were identified as E. coli in this current study. According serological and microbiological tests, 5(5.3%), 31(33.3%) and 7(7.5%) of E. coli isolates, characterized as Enterohemorrhagic E. coli (EHEC), STEC and attaching and effacing E. coli (AEEC) strains, respectively. High level resistance to tetracycline (89.8), ampicillin (82.8%) and sulfametoxazole-trimotoprime (71%) were detected among E. coli isolates. Analysis of ERIC-PCR results showed five different ERIC types among EHEC isolates. Based on our findings, chicken meat identified as a sources of STEC strains, therefore, the controlling and checkup the chicken meats for the resistance and virulent strains of E. coli should be consider as a crucial issues in public health.
Sildenafil attenuates pulmonary hypertension in acute porcine endotoxaemia
Authors: Kemper Daniella A.G. , Otsuki Denise A. , Maia Débora R.R. , Massoco Cristina O. , Marcasso Rogério A. , Júnior José Otávio Costa Auler , Fantoni Denise T. 
Published: July 2019

Abstract
Background

Pulmonary hypertension is a risk factor for morbidity and mortality in septic shock. Pulmonary hypertension increases the right ventricular afterload, which tends to reduce the right ventricular output, leading to right ventricular dysfunction. Sildenafil has been largely used in the treatment of pulmonary arterial hypertension, but its effects in sepsis are unknown. The aim of this study was to investigate the hypothesis that sildenafil is able to attenuate endotoxin-induced pulmonary hypertension in a porcine model. Further, we investigated the effects on hemodynamic and oxygenation functions. We also evaluated lung morphology and the effect on plasma cytokine and troponin response. Methods Twenty pigs in which endotoxeamia was induced through intravenous bacterial lipopolysaccharide endotoxin (LPS) infusion (4µg/kg/h) were randomly assigned to Control group (n=10) – which received saline solution; or to Sildenafil group (n=10) – which received sildenafil orally (100 mg) previous the endotoxeamia. Results LPS induced a significant pulmonary hypertension with an increase in pulmonary arterial pressure, pulmonary vascular resistance index, end-diastolic volume of the right ventricle and also a decrease in PaO2/FiO2. Pulmonary and systemic arterial pressures were significantly lower in the Sildenafil group and sildenafil prevented the LPS effect on the end-diastolic volume of the right ventricle. Sildenafil maintained oxygenation with superior PaO2/FiO2 and lower oxygen extraction rate, but had no effect on intrapulmonary shunt. All cytokines and troponin increased after LPS infusion in both groups similarly. The LPS induced lung lesions, but there were no intergroup differences in the histological scoring system. Conclusion Sildenafil attenuated endotoxin-induced pulmonary hypertension preserving right heart function and maintained oxygenation without effecting shunt fractioning. However, caution should be taken due to the potential systemic vasodilatory effect. Sildenafil did not attenuate lung lesions and did not presented an anti-inflammatory effect in a porcine model of endotoxaemia.
The potential role of network-oriented interventions for survivors of violence among refugees in Belgium
Authors: Ogbe Emilomo , Jbour Alaa , Rahbari Ladan , Unnithan Maya , Degomme Olivier 
Published: July 2019

Abstract
Introduction
Social support and social network members have been identified as an important factor in mitigating the effects of sexual and gender-based violence (SGBV) and improving the coping process for many survivors. Network oriented strategies have been advocated for among domestic violence survivors, as they help build on improving social support and addressing factors that alleviate repeat victimization. There are opportunities to implement such strategies among refugees, who are survivors of SGBV in asylum centres, however, this has not been fully explored. This study sought to identify key strategies and opportunities for developing peer-led and network-oriented strategies for mitigating the effects of SGBV among refugees at these centres.
Methods: 27 interviews, were conducted with service providers (n=14) and refugees (n=13) at three asylum centres in Belgium. Deductive coding was performed independently by two researchers based on pre-identified research questions, when new findings emerged outside the themes, open coding was done. Data triangulation was used with findings from observations at these centres over a period of a year.
Findings: Many of the refugees presented with PTSD or psychosomatic symptoms, because of SGBV or other trauma experienced during migration. Peer and family support were very influential in mitigating the effects and social costs of violence among the refugees by providing emotional and material support. Social assistants were viewed as an information resource that was essential for most of the refugees. Peer-peer support was identified as a potential tool for mitigating the effects of SGBV
Proteomics variations after short-term heat shock treatment reveal differentially expressed proteins involved in early microspore embryogenesis in cabbage (Brassica oleracea)
Authors: Su Henan , Chen Guo , Liu Xing , Yang Limei , Zhuang Mu , Zhang Yangyong , Wang Yong , Fang Zhiyuan , Lv Honghao 
Published: July 2019

Abstract
Background

Microspore embryogenesis (ME) provides an efficient way to breed crops. In many cases, short-term heat shock treatment can greatly increase the embryogenesis rate of brassicas. However, its molecular mechanism is largely unclear.

Results

To mine for the key genes, pathways and interplay in the underlying networks, we compared the proteomes of isolated microspores with samples pre-treated at 32 °C for 24 h and 25 °C for 24 h using two cabbage accessions (Zhonggan 628 and 87-534) showing extremely different embryogenic rates. The embryo yield was 0 and 19.7 embryos/bud for Zhonggan 628 at 25 °C and 32 °C, respectively, and was 0 for 87-534 at both temperatures. Using a label-free proteomics technology, more differentially expressed proteins (DEPs) were found for Zhonggan 628 (363 DEPs, 115 upregulated and 248 downregulated) than for 87-534 (282 DEPs, 162 upregulated and 120 downregulated). 97 DEPs specially identified only in Zhonggan 628 but not in 87-534 after heat-shock treatment were the key proteins that maybe related to heat shock-induced embryogenesis in vitro culture. Those 97 DEPs were mainly enriched in carbon metabolic process and protein synthesis and degradation process. Malate dehydrogenase (mMDH), sgt1 homolog B (SGT1), heat shock 70 kDa protein 5 (HSP70), and cell division control protein 48 homolog A (CDC48) may play an important role in cabbage embryogenesis and were identified based on pathway enrichment and protein−protein interaction analyses. In addition, changes in the abundance of 9 representative DEPs were correlated with their corresponding mRNA levels using qRT-PCR. Carbohydrate metabolism supplies the energy needed for the rapid growth that occurs during embryo development, and the folding of synthesized proteins or the refolding of damaged and unstable proteins occur, which may due to the stress induced by in vitro culture.

Conclusions

A set of putative proteins presumably specific for microspore embryogenesis induced by high temperature treatment were identified. In isolated microspore culture of cabbage, we present the first exposition of non-embryo and the embryo (induced by 32 °C heat shock treatment 24h) changes in the expression of specific proteins.
Prevalence of hypertension and related risk factors in central Iran: Results from Yazd Health Study
Authors: Mirzaei Masoud , Mirzaei Mohsen , Gholami Somayeh , Abolhosseini Hossein 
Published: July 2019

Abstract
Background: Hypertension, the most important risk factor for cardiovascular disease, is a public health concern in world. Its prevalence varies between countries, due to differences in lifestyle and other risk factors' profile across the regions. The aim of this study was to investigate the prevalence of hypertension and its related risk factors among adults in Yazd Greater Area.
Methods: This cross-sectional study is a community-based study of 20-69 year-old adults living in Yazd. The participants were selected by multi-stage random cluster sampling. A valid questionnaire completed in a home visit. Blood pressure (BP), Body Mass Index and Waist Circumference were measured by standard protocol. Self-reported hypertension was recorded. levels of Physical Activity (PA) were categorized by IPAQ-SF. Chi-square test was used for categorical variables to analyze the differences. Multivariate logistic regression was carried out to determine the association of risk factors to hypertension. All statistical analyses were performed using SPSS 16 software. A p-value less than 0.05 were considered statistically significant.
Results: With 95% responding(n=9975), half of adults have a low PA and two thirds were overweight or obese. Positive history of hypertension was 18.5% (21.9% in females vs. 15.2% in males). The mean systolic and diastolic BP were 126.5 ± 18.4 mmHg and 80.2 ± 12.5 mmHg, respectively. Prevalence of hypertension was 36% (34.7% for women and 37.3% for men). The prevalence of hypertension is reduced with higher education, more PA, lower BMI and no history of diabetes (P value < 0.0001). Higher BP is less common in smokers (P value < 0.0001). By logistic regression analysis, hypertension were higher among men (OR: 1.83, 95% CI: 1.64-2.03), eldest (OR: 5.15, 95% CI: 4.20-6.31), low-educated (OR: 1.40, 95% CI: 1.17-1.67) and diabetics (OR: 1.20, 95% CI: 1.05-1.38).Hypertension was doubled in obese. There was no significant relationship between PA level, place of residence, smoking and hypercholesterolemia with high BP.
Conclusion: Prevalence of hypertension in adults was high. By identifying common modifiable risk factors, health policy makers should prioritize health interventions to control risk factors. It is necessary to inform younger adult groups about ways to select healthy lifestyle and nutritional habits.
Zinc for Infection Prevention in Sickle Cell Anemia (ZIPS): Study Protocol for a Randomized Placebo-Controlled Trial in Ugandan children with Sickle Cell Anemia
Authors: Datta Dibyadyuti , Namazzi Ruth , Conroy Andrea L. , Cusick Sarah E. , Hume Heather A. , Tagoola Abner , Ware Russell E. , Opoka Robert O. , John Chandy C. 
Published: July 2019

Abstract
Background

Sickle cell anemia (SCA) is the most common inherited hemoglobinopathy worldwide. Infection is a major cause of illness and death in children with SCA, especially in sub Saharan Africa where an estimated 50-90% of affected children die before their fifth birthday. Interventions to reduce the incidence and severity of infections are needed urgently. A high proportion of adults and children with SCA are zinc-deficient, and zinc deficiency leads to impaired immunity and an increased risk of infection. Zinc supplementation has been shown to decrease the risk of infection in adolescents and adults, but there is no data on the effectiveness of zinc for prevention of infection in children <5 years of age with SCA.

Methods/Design

The study will be a randomized, placebo-controlled, double-blind clinical trial in which 250 Ugandan children 1.00-4.99 years of age with SCA will receive daily zinc supplementation (10 mg oral dispersible tablet) or identical placebo for 12 months.

Discussion

If this trial shows a reduction in severe or invasive infection incidence, it would be the basis for a multi-site, multi-country clinical trial to assess real-world safety and efficacy of zinc in African children with SCA. Since zinc is safe, inexpensive, and easy to administer, this trial has the potential to improve the health of hundreds of thousands of African children with SCA through reduction of infection-related morbidity and mortality.

Trial Registration

Clinicaltrials.gov identifier: NCT03528434. Registered on May 17, 2018
Protocol Version: 1.0. Date: Dec 11, 2017
Sponsor: Indiana University. Sponsor’s protocol identifier: 1712339562
Nutrient removal using spent coconut husks
Authors: Halfhide Trina , Lalgee Lorale J. , Singh Karen Seudat , Williams Joshua , Sealy Matthew , Manoo Anton , Mohammed Azad 
Published: July 2019

Abstract
Wastewater management poses major environmental and economic problems in many small island developing states (SIDs), such as Trinidad and Tobago. Conventional wastewater treatment using activated carbon (AC) from charcoal is very costly. However, using spent coconut husks can be a cheap, environmentally friendly alternative source of AC for removing nitrogen and phosphorus in the secondary effluent. This study considered thermal and non-thermal, chemical activation methods to create four different AC treatments. The treatments considered included: virgin AC (thermally activated only) and subsequent acid treatments. Acid treatments were considered as an acidic environment, optimized nitrate and phosphate removal and reduced hydroxyl ion competition. Although all treatments effectively removed nitrates, virgin AC was ineffective in PO43−-P removal when concentrations were less than 10 mg/L. This was probably due to naturally occurring PO43−-P leaching from the coconut husk. The differences in removal efficiencies and adsorption capacities were not significantly different between treatments. Thermal activation was sufficient to remove NO3−-N and PO43−-P greater than 10 mg/L in experiments with batch stock solutions and the secondary effluent. Virgin AC can therefore be used as a potential substitute for conventional AC.
Breast Cancer and Anesthesia
Authors: Raigon-Ponferrada Aida , Recio María E.D. , Guerrero-Orriach Jose L. , Malo-Manso Alfredo , Escalona-Belmonte Juan J. , Aliaga Marta R. , Fernández Alicia R. , García Francisco J.F. , Conejo Emilio A. , Cruz-Mañas Jose 
Published: July 2019

:
Breast cancer is a complex heterogeneous disease that is categorized into several histological and genomic
subtypes with relevant prognostic and therapeutical implications. Such diversity requires a multidisciplinary
approach for a comprehensive treatment that will involve surgeons, radiotherapists and medical oncologists.
Breast cancer is classified as either local (or locoregional), which stands for 90-95% of cases, or metastatic, representing
5% of cases.

:
The management of breast cancer will be determined by the stage of the disease. The treatment of local breast
cancer is based on surgery and/or radiotherapy. Systemic breast cancer requires chemotherapy and/or endocrine
and/or biological therapy.
Intraoperative Cultures in Reimplantation of a Two-Stage Protocol: Only 1 vs. At Least 2 Positive Microbiological Results
Authors: Cordero-Ampuero José , Ortega-Columbrans Ana , Garcia-Rey Eduardo , Garcia-Cimbrelo Eduardo 
Published: July 2019

Background:The main reason for using a two-stage exchange in Prosthetic Joint Infection (PJI) is that bacteria are completely eradicated in reimplantation surgery. However, reports of a positive culture in the second surgery are growing. The number of positive intraoperative cultures and their influence on final results is not well-established.Objectives:To compare epidemiological characteristics, infection recurrence and clinical evolution of patients with only onevs.at least two positive cultures based on our series of cases with positive cultures in reimplantation surgery.Material and Methods:Retrospective study of 55 patients was conducted prospectively. They were diagnosed with chronic PJI, treated with a two-stage protocol and at least three intraoperative cultures were obtained in the second stage. These cultures were negative in 28 patients. Fourteen patients showed two or more cultures with the same microorganism and they were denominated patients with positive cultures. Thirteen patients showed only one positive culture, and they were considered contaminated. Both groups of patients (positive cultures and contaminated ones) received the second cycle of oral antibiotics for 6 months. Functional results were evaluated with the Harris Hip Score (hips) or Knee Society Clinical Rating Score (KSCRS) (knees).Results:There were no significant differences between patients with positive or contaminated cultures for age (p=0.420) and sex (p=0.385). The knee was involved in 13/14 positive and in only 6/13 contaminated patients (p=0.013).Staphylococcus epidermidiswas the predominant isolate, but there were differences between positive (methicillin-resistant in 7/14 patients) and contaminated cultures (methicillin-sensitive in 6/13). There were no differences in the prevalence of polymicrobial cultures (p=0.785) or coincidence with cultures from the first stage (p=0.257). Three infection recurrences have appeared in patients with positive cultures (3/13, 21%) and none in patients with contaminated cultures. There are no differences in HSS or KSCRS when comparing final functional results between groups (p=0.411).Conclusion:The prevalence of positive cultures in reimplantation surgery is higher than expected (25%), and more frequent in women and in knee arthroplasties. The most frequently involved microorganism isStaphylococcus epidermidis, but antibiotic sensitivity varies between patients with positive cultures (methicillin-resistant) and those with contaminated cultures (methicillin-sensitive). There were no infection recurrences in patients with contaminated cultures, but those with positive cultures present a risk of over 20%.
Web Service Discovery Using Bio-Inspired Holistic Matching Based Linked Data Clustering Model for RDF Data
Authors: Mehrotra Manish K. , Kanungo Suvendu 
Published: July 2019

Introduction:
Resource Description Framework (RDF) is the de-facto standard language
model for semantic data representation on semantic web. Designing an efficient management of RDF
data with huge volume and efficient querying techniques are the primary research areas in semantic
web.

Methods:
So far, several RDF management methods have been offered with data storage designs and
query processing algorithms for data retrieval. However, these methods do not adequately address the
presence of irrelevant links that degrade the performance of web service discovery. In this paper, we
propose a Bio-inspired Holistic Matching based Linked Data Clustering (BHM-LDC) technique for efficient
management and querying of RDF data. This technique is essentially based on three algorithms
which are designed for RDF data storing, clustering the linked data and web service discovery respectively.
Initially, the BHM-LDC technique store the RDF dataset as graph based linked data.

Results and Discussion:
Then, an Integrated Holistic Entity Matching based Distributed Genetic
Algorithm (IHEM-DGA) is proposed to cluster the linked data. Finally, a sub-graph matching based
web Service discovery Algorithm that uses the clustered triples has been proposed to find the best web
services. Our experimental results reveal the performance of the proposed web service discovery approach
by applying on business RDF dataset.
Classification of Diabetes by Kernel Based SVM with PSO
Authors: Choubey Dilip K. , Tripathi Sudhakar , Kumar Prabhat , Shukla Vaibhav , Dhandhania Vinay K. 
Published: July 2019

Background:
The classification method is required to deduce possible errors and assist
the doctors. These methods are used to take suitable decisions in real world applications. It is
well known that classification is an efficient, effective and broadly utilized strategy in several applications
such as medical diagnosis, etc. The prime objective of this research paper is to achieve an
efficient and effective classification method for Diabetes.

Methods:
The proposed methodology comprises two phases: The first phase deals with t h e
description of Pima Indian Diabetes Dataset and Localized Diabetes Dataset, whereas in the second
phase, the dataset has been processed through two different approaches.

Results:
The first approach entails classification through Polynomial Kernel, RBF Kernel, Sigmoid
Function Kernel and Linear Kernel SVM on Pima Indian Diabetes Dataset and Localized Diabetes
Dataset. In the second approach, PSO has been utilized as a feature reduction method followed
by using the same set of classification methods used in the first approach. PSO_Linear
Kernel SVM provides the highest accuracy and ROC for both the above mentioned dataset.

Conclusion:
The present work consists of a comparative analysis of outcomes w.r.t. performance
assessment has been done PSO and without PSO for the same set of classification methods. Finally,
it has been concluded that PSO selects the relevant features, reduces the expense and computation
time while improving the ROC and accuracy. The used methodology could be implemented
in other medical diseases.