bluuebunny/crossref_metadata_2025_split huggingface dataset:
The expanded specificity and physiological role of a widespread N-degron recognin
Authors: Gao Xiaohui , Yeom Jinki , Groisman Eduardo A. 
Published: August 2019

Significance
Proteases control a variety of cellular processes. The protease adaptor ClpS recognizes substrates bearing particular amino acids at the N terminus of proteins in bacteria and bacteria-derived organelles. By identifying and characterizing natural ClpS substrates, we dramatically expand the specificity and physiological role of ClpS. We determined that ClpS recognizes a degron in proteins harboring isoleucine, leucine, phenylalanine, tryptophan, tyrosine, or valine at the fourth position from their N terminus and retaining the N-terminal methionine. Identified ClpS substrates have a variety of functions, including response to different stresses.
IFITM3 protects the heart during influenza virus infection
Authors: Kenney Adam D. , McMichael Temet M. , Imas Alexander , Chesarino Nicholas M. , Zhang Lizhi , Dorn Lisa E. , Wu Qian , Alfaour Omar , Amari Foued , Chen Min , Zani Ashley , Chemudupati Mahesh , Accornero Federica , Coppola Vincenzo , Rajaram Murugesan V. S. , Yount Jacob S. 
Published: August 2019

Significance
We discovered that IFITM3 prevents efficient dissemination and replication of influenza virus in heart tissue, thereby limiting cardiac fibrosis and electrical dysfunction during infection. Since IFITM3 polymorphisms are among the only human genetic factors that have been reproducibly associated with hospitalization and mortality during influenza virus infection, our findings are relevant to the serious threat of influenza virus infection to human health. Furthermore, IFITM3 KO mice provide one of the first models for studying cardiac complications of influenza.
Idiosyncratic neural coding and neuromodulation of olfactory individuality in
Drosophila
Authors: Honegger Kyle S. , Smith Matthew A.-Y. , Churgin Matthew A. , Turner Glenn C. , de Bivort Benjamin L. 
Published: August 2019

Significance
Individuality is a fundamental feature of behavior. For instance, the same smell or song may evoke very different responses in 2 individuals. What is the biological basis for these differences? While behavioral differences likely originate with differences in neural activity, little is known about how idiosyncratic behavioral differences are reflected in neural activity. We used statistical behavioral analysis and live brain imaging to assess idiosyncratic odor responses in fruit flies, and found that the same odors produce different behavioral responses across flies and that these odors evoke subtly different patterns of brain activity across flies. Moreover, neuromodulators and sets of neurons in the olfactory region of the fly’s brain directly modulate the degree of fly-to-fly behavioral variability in a flexible way.
Human-modified landscapes alter mammal resource and habitat use and trophic structure
Authors: Magioli Marcelo , Moreira Marcelo Zacharias , Fonseca Renata Cristina Batista , Ribeiro Milton Cezar , Rodrigues Márcia Gonçalves , Ferraz Katia Maria Paschoaletto Micchi de Barros 
Published: August 2019

SignificanceKnowledge of resource and habitat use by wildlife is essential to support conservation actions. Stable isotope analysis (SIA) is a useful method for the acquisition of this type of information. Samples for SIA can be obtained through indirect and noninvasive methods, which is favorable for studies of threatened species. We used SIA to compare the resource and habitat use and trophic structure of mammals between preserved areas and human-modified landscapes in a tropical rainforest. Our study shows that mammals in human-modified landscapes present an altered trophic structure and use food items from the agricultural matrix, while they depend on forest resources in preserved areas. Our findings stress the need for favorable management of the agricultural matrix to support wildlife survival.
Therapeutic genome editing of triple-negative breast tumors using a noncationic and deformable nanolipogel
Authors: Guo Peng , Yang Jiang , Huang Jing , Auguste Debra T. , Moses Marsha A. 
Published: August 2019

Significance
Triple-negative breast cancer (TNBC), which represents 12% of all breast cancers, is a devastating breast cancer subtype that occurs more frequently in women under 50 y of age, in African American women, and in individuals carrying a BRCA1 gene mutation. Because of the lack of therapeutic targets and limited treatment options, the prognosis for patients with TNBC remains the poorest of all patients with breast cancer. Here we report the synthesis and application of a novel CRISPR nanotherapeutic to effectively knock out Lipocalin 2 (Lcn2), a breast cancer-promoting gene, in TNBC tumors via in vivo genome editing, leading to a significant suppression of TNBC tumor growth. Our studies demonstrate that CRISPR genome editing is a promising targeted gene therapy approach for TNBC.
Encounter complexes and hidden poses of kinase-inhibitor binding on the free-energy landscape
Authors: Re Suyong , Oshima Hiraku , Kasahara Kento , Kamiya Motoshi , Sugita Yuji 
Published: August 2019

Significance
Molecular recognition via protein–ligand binding is essential for biomolecular functions. Current understanding of ligand recognition is mostly brought from a bound state structure resolved by X-ray crystallography. Using molecular dynamics simulation with enhanced sampling techniques, we here characterize multiple bound poses and transient encounter complexes of an inhibitor kinase on the statistically converged free-energy landscapes. Our simulations propose parallel binding pathways connected through encounter complexes, highlighting the importance of early binding processes. Determination of transient encounter complexes is still difficult experimentally, and the present finding helps to understand the process and widens the possibility of drug compound design.
Allosteric modulation of β-cell M3muscarinic acetylcholine receptors greatly improves glucose homeostasis in lean and obese mice
Authors: Zhu Lu , Rossi Mario , Cohen Amanda , Pham Jonathan , Zheng Hongchao , Dattaroy Diptadip , Mukaibo Taro , Melvin James E. , Langel Jennifer L. , Hattar Samer , Matschinsky Franz M. , Appella Daniel H. , Doliba Nicolai M. , Wess Jürgen 
Published: August 2019

SignificanceType 2 diabetes (T2D) has emerged as a major threat to human health worldwide. In T2D, pancreatic β cells are unable to release sufficient amounts of insulin to maintain physiological blood glucose levels. Acetylcholine (ACh) stimulates insulin release from mouse and human pancreatic β cells by activating a cell surface receptor known as M3muscarinic ACh receptor (M3R). In the present study, we show that a drug that binds to an allosteric site on the M3R (a site distinct from the ACh-binding site) greatly enhances ACh-induced, M3R-mediated insulin release and improves glucose homeostasis in obese, glucose-intolerant mice. This proof-of-concept study strongly suggests that positive allosteric modulators of M3R function may prove beneficial for the treatment of T2D.
Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice
Authors: Fujihara Yoshitaka , Noda Taichi , Kobayashi Kiyonori , Oji Asami , Kobayashi Sumire , Matsumura Takafumi , Larasati Tamara , Oura Seiya , Kojima-Kita Kanako , Yu Zhifeng , Matzuk Martin M. , Ikawa Masahito 
Published: August 2019

SignificanceWhile the emergence of gene modification technologies has produced major discoveries in biomedical sciences, the recent development of the CRISPR/Cas9 system has dramatically altered the trajectory of phenotypic analysis in animal models. In this study, we identified male-specific gene clusters (CstandPate) and family genes (GdpdandLypd) and found specific members to be required for male fertility, especially for sperm fertilizing ability. Our findings support the important roles of these proteins in sperm function and could be used to develop novel infertility treatments as well as contraceptives.
Positive selection in dNTPase SAMHD1 throughout mammalian evolution
Authors: Monit Christopher , Morris Elizabeth R. , Ruis Christopher , Szafran Bart , Thiltgen Grant , Tsai Ming-Han Chloe , Mitchison N. Avrion , Bishop Kate N. , Stoye Jonathan P. , Taylor Ian A. , Fassati Ariberto , Goldstein Richard A. 
Published: August 2019

Significance
Animals defend themselves from viral infection using innate immunity proteins that disrupt various stages of the virus life cycle. In response, viruses produce proteins that bind these host factors and compromise their activity, resulting in evolutionary conflict as immunity and virus proteins adapt to prevent and restore binding, respectively. We report that evolutionary conflict involving the host innate immunity protein SAMHD1 has occurred throughout mammalian evolution. We observe adaptation in a region of SAMHD1 that regulates its activity, and we demonstrate how mutations here influence its enzymatic properties, suggesting that evolutionary conflict has involved modulation of SAMHD1 regulation and function. This correlates with reduced restriction of HIV-1, indicating that positive selection has influenced both SAMHD1’s dNTPase and antiviral activities.
Carbon-13 dynamic nuclear polarization in diamond via a microwave-free integrated cross effect
Authors: Henshaw Jacob , Pagliero Daniela , Zangara Pablo R. , Franzoni María B. , Ajoy Ashok , Acosta Rodolfo H. , Reimer Jeffrey A. , Pines Alexander , Meriles Carlos A. 
Published: August 2019

Significance
Optical spin pumping of color centers promises to revolutionize the practice of nuclear magnetic resonance via efficient dynamic nuclear polarization protocols. Significant progress has been attained with systems such as the nitrogen-vacancy (NV) center in diamond, but extreme spectral broadening and nuclear spin-lattice relaxation from coexisting paramagnetic defects are hurdles still difficult to circumvent. The present technique addresses both issues through the implementation of magnetic-field-sweep–induced NV cross-relaxation with ancilla point defects, here acting as proxies to mediate the transfer of polarization to the nuclear spin ensemble. The governing spin dynamics is robust to field misalignment and ideally suited for shallow NVs, indirectly coupled to outside nuclei through paramagnetic defects at the diamond surface.
Neurodevelopmental mutation of giant ankyrin-G disrupts a core mechanism for axon initial segment assembly
Authors: Yang Rui , Walder-Christensen Kathryn K. , Lalani Samir , Yan Haidun , García-Prieto Irene Díez , Álvarez Sara , Fernández-Jaén Alberto , Speltz Laura , Jiang Yong-Hui , Bennett Vann 
Published: August 2019

Significance
Axon initial segments of vertebrate neurons integrate thousands of dendritic inputs and generate a single outgoing action potential. Giant ankyrin-G associates with most of the molecular components of axon initial segments and is required for their assembly. This study identified 3 human mutations of giant ankyrin-G resulting in impaired neurodevelopment in compound heterozygotes. These mutations prevent transition of giant ankyrin-G from a closed to an open conformation, which normally is regulated by phosphorylation of giant ankyrin-G during maturation of axon initial segments. Giant ankyrin-G thus functions in a signaling pathway that may contribute to activity-dependent plasticity of the axon initial segment as well as provide a therapeutic target for treatment of patients bearing giant ankyrin-G mutations.
Spontaneous generation of hydrogen peroxide from aqueous microdroplets
Authors: Lee Jae Kyoo , Walker Katherine L. , Han Hyun Soo , Kang Jooyoun , Prinz Fritz B. , Waymouth Robert M. , Nam Hong Gil , Zare Richard N. 
Published: August 2019

Significance
Water is considered to be a stable and relatively inert molecule in bulk solution. We report an exceptional behavior of water: Water molecules are spontaneously oxidized to form hydrogen peroxide near the water−air interface of micron-sized water droplets. This process does not require any chemical reagent, catalyst, applied electric potential, or radiation. Only pure water in the form of microdroplets in air is necessary for the appearance of hydrogen peroxide. We suggest that this discovery opens various innovative opportunities including green and inexpensive production of hydrogen peroxide, green chemical synthesis, safe cleaning, and food processing.
15 years of collostructions
Authors: Gries Stefan Th. 
Published: August 2019

AbstractThis paper discusses a variety of potential shortcomings of most of the most widely-used association measures as used in collocation research and collostructional analyses. To address these shortcomings, I then discuss a research program calledtupleization, an approach that does away with the usual kinds of information conflation by keeping relevant corpus-linguistic dimensions of information – e.g. frequency, association/contingency, dispersion, entropy, etc. – separate and analyzing them in a multidimensional way; I conclude with pointers towards how these dimensions could, if deemed absolutely necessary, be conflated for the simplest kinds of of rankings as well as strategies for future research.
A corpus perspective on the development of verb constructions in second language learners
Authors: Römer Ute 
Published: August 2019

AbstractThis article reports initial findings from a study that uses written data from second language (L2) learners of English at different proficiency levels (CEFR A1 to C1) in a large-scale investigation of verb-argument construction (VAC) emergence. The findings provide insights into first VACs in L2 learner production, changes in the learners’ VAC repertoire from low to high proficiency levels, and changes in learners’ dominant verb-VAC associations from low to high proficiency levels. The article also addresses the question what role formulaic sequences play in the L2 acquisition of VACs. Data analyses indicate that, from lowest to highest proficiency levels, the VAC repertoire of L2 English learners shows an increase in VAC types, growth in VAC productivity and complexity, and a development from predominantly fixed sequences to more flexible and productive ones. The findings help to expand our understanding of the processes that underlie construction acquisition in an L2 context.
Construction Grammar and the corpus-based analysis of discourses
Authors: Groom Nicholas 
Published: August 2019

AbstractConstruction grammar (CxG) initially arose as a usage-based alternative to nativist theoretical accounts of language, and remains to this day strongly associated with cognitive linguistic theory and research. In this paper, however, I argue that CxG can be seen as offering an equally viable general framework for socially-oriented linguists whose work focuses on the corpus-based analysis of discourses (CBADs). The paper begins with brief reviews of CxG and CBADs as distinctive research traditions, before going on to identify synergies (both potential and actual) between them. I then offer a more detailed case study example, focusing on a usage-based analysis of a newly identified construction, the WAY IN WHICH construction, as it occurs in corpora representing six different academic discourses. The paper concludes by rebutting some anticipated objections to the approach advocated here, and by proposing a new conceptual model for constructionist approaches to CBADs.
Towards an English Constructicon using patterns and frames
Authors: Perek Florent , Patten Amanda L. 
Published: August 2019

Abstract
Recent research in construction grammar has been marked by increasing efforts to create constructicons: detailed
inventories of form-meaning pairs to describe the grammar of a given language, following the principles of construction grammar.
This paper describes proposals for building a new constructicon of English, based on the combination of the COBUILD Grammar
Patterns and the semantic frames of FrameNet. In this case study, the valency information from FrameNet was automatically matched
to the verb patterns of COBUILD, in order to identify the frames that each pattern is associated with. We find that the automatic
procedure must be complemented by a good deal of manual annotation. We examine the “V that” pattern in particular, illustrating
how the frame information can be used to describe this pattern in terms of constructions.
Impolite viewer responses in Arabic political TV talk shows on YouTube
Authors: Hassan Bahaa-eddin A. 
Published: August 2019

Abstract
The article deals with the features of impolite responses of YouTube Arab viewers of political TV talk shows.
YouTube comments are written discourse of live commentary, a new genre of computer-mediated communication. Based on data from
comments of Arabic viewers of political TV talk shows on YouTube, the article argues that impolite responses appear to be a common
feature in Arabic comments in political talk shows on YouTube. Identity and power are reconsidered in this paper as variables that
trigger impoliteness in Arabic online responses in political talk shows on YouTube. It argues that obscuring identity online
incites the use of conventionalized impoliteness to exercise power on the TV presenter or the TV episode’s guest. The article also
shows how communication variables such as context, commentator’s identity and models of communication influence the realization of
impolite responses in those online interactions. The study draws on Spencer-Oatey
(2007) to correlate identity, power, and impoliteness. It also utilized Culpeper’s
(2011) bottom-up model of impoliteness triggers.
Combinaciones entre operadores argumentativos
Authors: Fuentes Rodríguez Catalina 
Published: August 2019

ResumenEl trabajo analiza la fijación de una construcción,si cabe, como operador escalar. Se describe su comportamiento como regulador de la escala: señala la posición superior y exige la combinación con otro intensificador. Se analiza también la aparición de la estructura en diferentes contextos, ya sea como construcción libre o construcción con valor periférico en el enunciado. La descripción sigue una perspectiva pragmática y macrosintáctica, se centra en el estudio de la gramaticalización de operadores discursivos e incorpora a su estudio una dimensión combinatoria de la que surgen nuevos elementos. La investigación se apoya en los corpus de la RAE (CREA, CORPES y CORDE) así como en el corpus MEsA, de discurso digital.
‘Is it always so fast?’
Authors: Zhang Leticia Tian , Cassany Daniel 
Published: August 2019

AbstractWhile much research has proved the benefits of subtitled audiovisuals for foreign language learning, few studies address such practices in out-of-classroom settings or focus on Asia-based video-sharing platforms. This study bridges this gap by introducing an increasingly popular viewing-commenting system in Japan and China, known asdanmuordanmaku, which displays viewers’ timeline-synchronized comments on video content. We analyse the metalinguistic comments which entail viewers’ knowledge of the language, their comprehension issues and sociolinguistic attitudes toward its use. Adopting an inductive or data-driven methodology, we extracted and manually coded 390 comments that are related to the Spanish language, Spanish–Chinese translation and learning Spanish. Results show that viewers are mostly interested in linguistic features that differ from Chinese or English (e.g. the complex grammar) and they usedanmuto access sociolinguistic issues that are central to daily communication such as the fast speech rate, language varieties, and frequent use of vulgarisms.
La campaña contra el ‘manspreading’ entre paisaje semiótico y eco mediático
Authors: Pontrandolfo Gianluca 
Published: August 2019

Resumen
El estudio analiza la campaña contra el ‘manspreading’ (el despatarre masculino en el transporte público) como
fenómeno semiótico. El punto de partida de la investigación es la decisión de la Empresa Municipal de Transportes de Madrid de
poner una pegatina contra el despatarre de los hombres en todos los autobuses madrileños. El tema despierta interés y desata
críticas desde distintas posiciones: la supuesta invasión del espacio público por parte de los varones engendra reflexiones acerca
del posicionamiento de los hombres y de las mujeres en la sociedad moderna; además, estos gestos de comunicación no verbal y los
signos lingüísticos a estos asociados revelan sesgos de género que es interesante observar bajo una perspectiva sociolingüística y
multimodal.
Desde el punto de vista metodológico, la investigación sienta sus bases en dos ejes: por un lado, los cimientos
teóricos del ‘paisaje lingüístico’, al analizar algunas imágenes contra el ‘manspreading’ como productos semióticos sociales de
corte multimodal; por el otro, el estudio del discurso enfocado bajo la perspectiva sociolingüística y pragmática, al examinar la
resonancia de la decisión en la prensa española.
Analysis of Risk Assessment of Mount Merapi Eruption in Settlement Area of Sleman Regency
Authors: Nugraha A L , Hani’ah  , Firdaus H S , Haeriah S 
Published: August 2019

Abstract
Mount Merapi is one of the most active volcanoes in Indonesia. Seeing the status of Mount Merapi which until now is still quite active, disaster mitigation action efforts are needed. One of the disaster mitigation actions is by making the Risk Map. This study aims to provide an assessment of risk assessment of eruption of Mount Merapi in settlement areas so as to minimize losses that can occur. Mapping of eruption risk of Mount Merapi using two parameters, namely hazard and vulnerabilities. Risk assessment is derived from Matrix comparison of Risk Assessment. Making the risk map using processing method and spatial analysis of Geographic Information System (GIS) with scoring method, weighting, and overlay. The addition of settlement information was obtained using the Normalized Difference Built-up Index (NDBI) method to know total settlement areas. This research will produce a risk settlement area map of Mount Merapi eruption and conclusion that there is still a large risk that occurs in Sleman regency. And Remote sensing technology and GIS can answer challenges in providing disaster analysis with a fast response and better accuracy so that the need for other implementations in building disaster maps in other regions with this technology.
Spatial Analysis of Flood and Landslide Vulnerable Areas (Case Study in Trenggalek Regency)
Authors: Riadi B , Windiastuti R , Suwarno y 
Published: August 2019

Abstract
Floods and landslides occurred almost every year in various regions in Indonesia, especially in hilly or bumpy areas. Floods and landslides as natural phenomena may cause severe disaster, due to high rainfall with small capacity of river cross section that resulted in water overflow through the dike and inundated lower areas. Flood and landslide risk mapping provides spatial information as initiation for mitigation purposes and is very beneficial in minimizing casualties and damages. To avoid landslide disaster, we need to reduce some causing factors, such as the factors that cause unstable slope conditions. The landslide distribution map contains information on geological maps, slope class maps, land cover maps and geomorphological maps; these maps are referred to as factor maps. Overlay method was implemented to analyse flood and landslide-prone areas. The purpose of this paper was to obtain information on areas in Trenggalek Regency that were vulnerable to flood and landslide. Risk analysis was done spatially with good accuracy, so that it can be used for disaster mitigation purposes.