bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kliničnopsihološka obravnava otrok in mladostnikov s tumorjem osrednjega živčnega sistema
Authors: Uršič Katarina , Bürger Lazar Martina 
Published: October 2019

V Sloveniji v povprečju letno zboli okoli 20 otrok in mladostnikov s tumorji osrednjega živčnega sistema (tumorji OŽS), kar predstavlja približno 20 % vseh bolnikov, zdravljenih na Kliničnem oddelku za otroško hematologijo in onkologijo Pediatrične klinike (KOOHO). Otroci s tumorji OŽS potrebujejo celostno interdisciplinarno obravnavo, del katere je tudi ustrezna kliničnopsihološka diagnostika in celostna nevrokognitivna rehabilitacija, ki je zaradi zelo pogostih kognitivnih in psihosocialnih posledic bolezni ter zdravljenja, bistvenega pomena. Izvaja jo klinični psiholog, ki je član stalnega tima KOOHO. Kliničnopsihološko in nevropsihološko spremljanje otroka se prične že med samim zdravljenjem, po zaključenem zdravljenju in nato sledi redno spremljanje na 2 – 3 leta oziroma ob opozorilu staršev ali lečečega pediatra onkologa na pomembne spremembe v funkcioniranju otroka. Ocenjujemo splošno inteligentnost, šolske dosežke, izvršilne funkcije, pozornost in koncentracijo, spomin in učenje, nevro-senzorično zaznavo, vidno-prostorske in vidno-gibalne sposobnosti, zaznavno procesiranje, hitrost procesiranja, govor, prilagoditvene spretnosti, čustvovanje, vedenje in kakovost življenja. S prepoznavo vrste in resnosti dolgoročnih posledic bolezni in zdravljenja, lahko otroku ali mladostniku nudimo individualna priporočila za ukrepe in prilagoditve ter ustrezno zgodnjonevrorehabilitacijo, ki ima pomembno vlogo pri razvoju neodvisnosti in avtonomnosti v odrasli dobi.
Kakovostna celostna obravnava otrok s tumorji OŽS vpliva na preživetje bolnikov, zmanjšuje pozne posledice zdravljenja in omogoča otrokom in kasneje odraslim čim boljšo kakovost življenja na področju zdravja, izobraževanja, zaposlitve, partnerskih in družinskih odnosov. Pri otrocih in mladostnikih z neozdravljivim možganskim tumorjem pa je pomembna zgodnja vključitev v interdisciplinarno paliativno oskrbo, z namenom vzdrževanja optimalne kakovosti življenja bolnika in svojcev v dani situaciji. Gre zaaktivno, celostno oskrbo bolnikov ter pomoč njihovim bližnjim tako v času bolezni kot tudi v procesu žalovanja.
CELSR3 mRNA expression is increased in hepatocellular carcinoma and indicates poor prognosis
Authors: Gu Xuefeng , Li Hongbo , Sha Ling , Mao Yuan , Shi Chuanbing , Zhao Wei 
Published: October 2019

Objective
Hepatocellular carcinoma (HCC) is a disease that is associated with high mortality; currently, there is no curative and reliable treatment. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) is the key signaling molecule in the wingless and INT-1/planar cell polarity (WNT/PCP) pathway. This study aimed to elucidate the prognostic significance of CELSR3 in HCC patients.

Methods
The Cancer Genome Atlas (TCGA) database, the Cancer Cell Line Encyclopedia (CCLE) database and the Gene Expression Omnibus (GEO) database were used to analyze the expression of CELSR3 mRNA in HCC samples and cells. The relationship between CELSR3 mRNA and clinical features was assessed by the chi-square test. the diagnostic and predictive value of CELSR3 mRNA expression were analyzed using the receiver operating characteristic (ROC) curve. Kaplan–Meier curve and Cox regression analyses were performed to assess the prognostic value of CELSR3 mRNA in HCC patients. Finally, all three cohorts database was used for gene set enrichment analysis(GSEA) and the identification of CELSR3-related signal transduction pathways.

Results
The expression of CELSR3 mRNA was upregulated in HCC, and its expression was correlated with age (P = 0.025), tumor status (P = 0.022), clinical stage (P = 0.003), T classification (P = 0.010), vital status (P = 0.001), and relapse (P = 0.005). The ROC curve assessment indicated that CELSR3 mRNA expression has high diagnostic value in HCC and in the subgroup analysis of stage. In addition, the Kaplan-Meier curve and Cox analyses suggested that patients with high CELSR3 mRNA expression have a poor prognosis, indicating that CELSR3 mRNA is an independent prognostic factor for the overall survival of HCC patients. GSEA showed that GO somatic diversification of immune receptors, GO endonuclease activity, GO DNA repair complex and GO somatic cell DNA recombination, were differentially enriched in the meta-GEO cohort, the HCC cell line cohort and the TCGA cohort of the high CELSR3 mRNA expression phenotype.

Conclusion
Our results indicate that CELSR3 mRNA is involved in the progression of cancer and can be used as a biomarker for the prognosis of HCC patients.
Magnetic and Electric Control of Circularly Polarized Emission through Tuning Chirality‐Generated Orbital Angular Momentum in Organic Helical Polymeric Nanofibers
Authors: Wang Zhongxuan , Gao Mingsheng , Ren Shenqiang , Hao Xiaotao , Qin Wei 
Published: October 2019

AbstractCircularly polarized light emission promotes the development of smart photonic materials for advanced applications in chiral sensing and information storage. The orbital angular momentum is a unique property for organic chiral helical materials. In this work, a type of organic chiral polymeric nanowires is designed with strong chirality induced orbital angular momentum. Under the stimulus of an external magnetic field of 600 mT, circularly polarized emission from the chiral polymeric nanowire becomes more pronounced, where the g factor increases from 0.21 to 0.3. The observed phenomena mainly originate from the chirality‐dependent orbital angular momentum. Moreover, the orbital angular momentum in helical chiral nanowire structures can be suppressed by inhibiting electron transport in a helical way to diminish circularly polarized light emission at room temperature.
Cofunction of Protons as Dopant and Reactant Activate the Electrocatalytic Hydrogen Evolution in Emeraldine‐Polyguanine
Authors: Coskun Halime , Aljabour Abdalaziz , Schöfberger Wolfgang , Hinterreiter Andreas , Stifter David , Sariciftci Niyazi Serdar , Stadler Philipp 
Published: October 2019

AbstractFunctional conductive polymers represent an emerging class of nonmetallic electrocatalysts attractive to substitute scarce and expensive elements used today. Here the synthesis of emeraldine‐polyguanine as structural‐molecular analog to polyaniline is shown and the conducting biopolymer as molecular hydrogen electrocatalyst is applied. The polymerized and protonated nucleobase aids the catalytic electroreduction of protons over a peculiar mechanism, where, over amino‐association, protons coserve as dopant and reactant. In acidic media the electrocatalytic evolution of hydrogen at a Tafel slope of 79 mV dec−1 at 290 mV overpotential (10 mA cm−2) is reported. The 140h initial stability test demonstrates that the functionality of polymerized nucleobases can be harnessed for heterogeneous catalysis, i.e., as here demonstrated for the electroconversion of hydrogen.
Bioactive Hydrogel Platforms for Spatiotemporal Delivery of Baculoviruses in Biomedical Applications
Authors: Whitlow Jonathan , Pacelli Settimio , Walston Towne , Paul Arghya 
Published: October 2019

AbstractBaculoviruses offer a multitude of advantages over other gene carriers due to their non‐pathogenicity and inability to replicate in mammalian cells, ease of production, and high transduction efficiencies. To expand their functionality in vivo, baculoviruses containing mammalian transcription promoters (BacMam) can be physically entrapped within biocompatible hydrogels to modulate their release and transduction efficiency. The present work demonstrates several techniques in which BacMam viruses can be encapsulated and delivered in fibrin gel, an FDA‐approved sealant and adhesive with numerous clinical applications, to provide spatiotemporal control over the delivery of BacMam viruses to mammalian cells. Fibrin is shown to be an effective biomaterial carrier for the delivery of BacMam viruses to mammalian cells growing in 2D culture, 3D culture, and in the co‐delivery of stem cells and baculoviruses in the aerosolized delivery of fibrin films. Overall, the results of this work provide a valuable insight on the therapeutic potential of fibrin gels for in vivo BacMam‐mediated gene delivery in regenerative medicine.
Molecular Architecture and Charging Effects Enhance the In Vitro and In Vivo Performance of Multi‐Arm Antimicrobial Agents Based on Star‐Shaped Poly(l‐lysine)
Authors: Lu Chao , Quan Guilan , Su Ma , Nimmagadda Alekhya , Chen Weidong , Pan Miao , Teng Peng , Yu Feiyuan , Liu Xi , Jiang Ling , Du Wenyi , Hu Wei , Yao Fen , Pan Xin , Wu Chuanbin , Liu Daojun , Cai Jianfeng 
Published: October 2019

AbstractMolecular architecture is a largely neglected and unexplored factor that could impart a significant difference to the antimicrobial activity of antimicrobial peptides. In this article, the advantages (i.e., improved charging effect and enhanced proteolytic stability) of star‐shaped poly(l‐lysine)s (PLLs) over their linear analogs are extensively explored by the methods of computational simulation and experiments. A series of PEI‐g‐PLL with a hyperbranched polyethylenimine (PEI) core and PLL peripheral chains are designed as a class of versatile molecular scaffold for the development of star‐shaped antimicrobial peptides. Computational simulations and zeta‐potential measurements reveal that the change in PLL conformation from linear to star‐shaped significantly increases the cationic charge density, allowing enhanced binding affinity toward the bacterial membrane. The minimum inhibitory concentration and killing kinetics measurements demonstrate that PEI‐g‐PLLs exhibit higher antimicrobial activity and bactericidal efficiency in vitro than the linear PLL counterparts. The absence of hydrophobic segments in PEI‐g‐PLLs weakens the nonspecific interactions with eukaryotic cells and offers remarkable selectivity, as evidenced by their negligible hemolytic activity. Furthermore, PEI‐g‐PLLs demonstrate enhanced proteolytic stability and unprecedented antimicrobial activity in vivo. PEI‐g‐PLLs, with their high antimicrobial activity, enormous selectivity, and remarkable proteolytic stability, represent a new series of potent antimicrobial peptides to treat drug‐resistant infections.
Gene‐Hydrogel Microenvironment Regulates Extracellular Matrix Metabolism Balance in Nucleus Pulposus
Authors: Chen Wei , Chen Hao , Zheng Dandan , Zhang Hongbo , Deng Lianfu , Cui Wenguo , Zhang Yuhui , Santos Hélder A. , Shen Hongxing 
Published: October 2019

AbstractGene therapy provides an ideal potential treatment for intervertebral disk degeneration by delivering synthetic microRNAs (miRNAs) to regulate the gene expression levels. However, it is very challenging to deliver miRNAs directly, which leads to inactivation, low transfection efficiency, and short half‐life. Here, Agomir is loaded in hydrogel to construct a gene‐hydrogel microenvironment for regulating the synthesis/catabolism balance of the tissue extracellular matrix (ECM) to treat degenerative diseases. Agomir is a cholesterol‐, methylation‐, and phosphorothioate‐modified miRNA, which can mimic the function of miRNA to regulate the expression of the target gene. Agomir874 that mimics miRNA874 is synthesized to down regulate the expression of matrix metalloproteinases (MMPs) in nucleus pulposus (NP). At the same time, a polyethylene glycol (PEG) hydrogel is synthesized through Ag‐S coordination of 4‐arm PEG‐SH and silver ion solution, which has injectable, self‐healing, antimicrobial, degradable, and superabsorbent properties and matches perfectly with the mechanism of intervertebral disk. By delivering Agomir‐loaded PEG‐hydrogel to a degenerative intervertebral disk, a gene‐hydrogel microenvironment is constructed in situ, which reduces the expression of MMPs, regulates the synthesis/catabolism balance of ECM in the NP of the intervertebral disk, and improves the tissue microenvironment regeneration.
Quantum Probing Topological Phase Transitions by Non‐Markovianity
Authors: Giorgi Gian Luca , Longhi Stefano , Cabot Albert , Zambrini Roberta 
Published: October 2019

AbstractUnderstanding the physical significance and probing the global invariants characterizing quantum topological phases in extended systems is a main challenge in modern physics with major impact in different areas of science. Here, a quantum‐information‐inspired probing method is proposed where topological phase transitions are revealed by a non‐Markovianity quantifier. The idea is illustrated by considering the decoherence dynamics of an external read‐out qubit that probes a Su–Schrieffer–Heeger (SSH) chain with either pure dephasing or dissipative coupling. Qubit decoherence features and non‐Markovianity measure clearly signal the topological phase transition of the SSH chain.
Preparation of novel hydrophobic magnetic Fe3O4/waterborne polyurethane nanocomposites
Authors: Yang Lei , Fu Qi , Fu Heqing 
Published: October 2019

ABSTRACTMagnetic Fe3O4/waterborne polyurethane nanocomposites were synthesized based on waterborne polyurethane (WPU) and amino‐functionalized Fe3O4 by in situ polymerization. The Fe3O4 nanoparticle was found to be uniformly distributed in Fe3O4/WPU nanocomposites with linear or crosslinked structure. In addition, the formation mechanism and magnetic conduction mechanism of stable inorganic–organic nanocomposites were discussed. The experimental results showed that the thermal stability, magnetic, and mechanical properties of magnetic Fe3O4/waterborne polyurethane nanocomposites were improved by amino functionalized Fe3O4. Furthermore, the defoaming property of the emulsion and the hydrophobic property of magnetic Fe3O4/waterborne polyurethane nanocomposites were improved by the 1‐hexadecanol‐terminated prepolymer. What more, polycaprolactone (PCL)‐based Fe3O4/WPU nanocomposites have excellent mechanical properties (The tensile strength is over 30 MPa, the elongation rate is above 300%.) and magnetic properties. Magnetic Fe3O4/waterborne polyurethane nanocomposites will be used in the field of hydrophobic and microwave absorbent materials. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48546.
Evaluating the ion exchange chromatography for matrix‐assisted PEGylation and purification of consensus interferon
Authors: Bajwa Fakiha , Ahmed Nadeem , Khan Mohsin Ahmad , Azam Farhana , Akram Muhammad , Tahir Saad , Zafar Ahmad Usman 
Published: October 2019

AbstractScientists have implemented protein‐PEGylation technology for boosting‐up the pharmacokinetics and stability of recombinant therapeutic proteins. In the present study, (a) matrix‐assisted PEGylation was compared with solution‐phase PEGylation and (b) matrix‐assisted PEGylation was performed with different ion exchange resins for impact of chromatography medium on yield and purity of PEGylated product. DEAE Sepharose CL 6B, DEAE Fracto gel, and Macro cap Q ion exchange chromatography medium were compared for on column PEGylation and purification of cIFN. A MSC‐PEG of 12.0 KDa was selected. cIFN was bound to ion exchange medium, and PEG solution was passed through resin for 180 Min, and protein was eluted by sodium chloride linear gradient. Yield and purity for mono‐PEGylated cIFN with Macro cap Q matrix was 75% and 99%, respectively, whereas for DEAE Sepharose was 45% and 60%. DEAE Fracto gelTM purity was 85% with 50% yield of mono‐PEGylated cIFN. Further investigation of in vitro biological activities demonstrated that about 30% antiviral activity was reduced as compared to unmodified cIFN. However, thermal stability was significantly improved. The present study proved that matrix‐assisted PEGylation can improve the yield and purity of mono‐PEGylated product, and Macro Cap resin provided the highest yield of a homogeneous product.In present study, (a) matrix‐assisted PEGylation was compared with solution‐phase PEGylation and (b) matrix‐assisted PEGylation was performed with different ion exchange resins for impact of chromatography medium on yield and purity of PEGylated product. Matrix‐assisted PEGylation increases the yield of mono‐PEGylated product and further Macro CapTM produced highest yield and purity of PEGylated cIFN.
Einbeziehung der Öffentlichkeit und Kommunikation bei Großprojekten
Authors: Knorr Susanne , Möller Christian , Schulz Christian 
Published: October 2019

AbstractDie Erwartungshaltung der Öffentlichkeit hat sich in den letzten Jahren geändert: Die formelle Beteiligung von Bürgerinnen und Bürgern, die durch Infrastrukturmaßnahmen betroffen sind, greift zu kurz. Betroffene und Nutzer möchten nicht nur frühzeitig und umfangreich informiert werden, sondern bisweilen sogar selbst an der Planung mitwirken. Dieser Anspruch, der positive Auswirkungen auf das jeweilige Vorhaben beinhaltet, muss in den Projektablauf integriert werden. Technische Planer, Projektmanager und Verantwortliche für die Kommunikation müssen gut zusammenarbeiten. Ein Projektdesign, das eine enge Verzahnung von Dialog und Projektmanagement über den gesamten Projektzeitraum vorsieht, lohnt sich. Die Vorteile der geänderten Herangehensweise sind weit mehr als die Steigerung der öffentlichen Akzeptanz von Infrastrukturprojekten. Mögliche Konflikte, die im weiteren Verfahren zu Protesten führen können, werden frühzeitig sichtbar, wenn ein Eingriff in die Planungen noch nicht ausgeschlossen ist. Verzögerungen im Projektablauf können verringert werden, was auch zur Wirtschaftlichkeit beiträgt.Doch wie können Stakeholder gewinnbringend in die Planung integriert werden? Welcher Ansatz ist für das jeweilige Projekt der vielversprechendste? Reicht umfangreiche Information der Stakeholder, ist ein kontinuierlicher Dialog sinnvoll oder sind Formate der Beteiligung am aussichtsreichsten? Eine gute Orientierung bieten die VDI Richtlinien 7000/7001.
Der Stahlüberbau des Hochmoselübergangs – konsequent ganzheitliche Lösung
Authors: Käsmaier Manfred , Heiland Uwe 
Published: October 2019

AbstractDie Errichtung der Hochmoselbrücke führte zu einer der größten Brückenneubauten Deutschlands der letzten Jahrzehnte. Mit Parametern wie Bauhöhe über Grund von 158 m, der Gesamtlänge von 1 704 m und der max. Einzelstützweite von rd. 210 m, die auch während des Montagevorgangs nicht durch Hilfsabstützungen zu reduzieren war, verlässt die Hochmoselbrücke übliche Dimensionen des Ganzstahlbrückenbaus. Durch den im Taktschiebeverfahren hergestellten Überbau wurde mit Überwindung der max. Einzelstützweite von 210 m ein in Europa bislang nicht erreichter freier Kragarm ausgeführt. Die hohen Pfeiler, die sehr große Schlankheiten aufweisen, stellten eine weitere Schwierigkeit beim Taktschieben dar. Es zeigte sich, dass technische Lösungen für Konstruktionen, bei denen maßgebliche Parameter den Bereich üblicher Dimensionen verlassen, als oft nicht kompatibel für die Anwendung der gewöhnlichen Konstruktions‐ und Dimensionierungsprinzipien sind. Bei scheinbarer phänomenologischer Analogie zweier zu vergleichender Konstruktionen bei gleichzeitiger offensichtlicher Maßstabsveränderung ist keine ungeprüfte Übertragung von Erfahrungswerten in Bezug auf die Auslegung einer Konstruktion oder Montageverfahren möglich, sondern sind sinnvolle Sonderlösungen anzuwenden. Durch die vorhandenen Dimensionen stellten Herstellung und Bau dieser Großbrücke in vieler Hinsicht eine Herausforderung für alle am Projekt Beteiligten dar und führten zu zahlreichen innovativen Entwicklungen und Lösungen in der Montagetechnologie.
Gründung und Unterbauten der Hochmoselbrücke – Planung und Ausführung
Authors: Schmidt‐Hurtienne Björn , Krumbein Olaf 
Published: October 2019

AbstractMit einer Höhe von 160 m über Grund und Einzelstützweiten bis 209,5 m befindet sich die Hochmoselbrücke im Grenzbereich der Brückenbaunormen. Geologisch anspruchsvoll wird die Brücke am Westufer der Mosel in einem rutschgefährdeten Steilhang tief gegründet. Die schlanken Pfeiler erfahren neben hohen Windbeanspruchungen auch eine Anregung durch wirbelerregte Querschwingungen. Um unter Beachtung dieser Faktoren konstruktive Lösungen zu entwickeln, waren bei der Planung und Ausführung der Gründungsbauteile und der Pfeiler im Bau‐ und Endzustand ergänzende Untersuchungen einschließlich Windkanalversuchen erforderlich. Die aeroelastische Stabilisierung der Pfeiler gegen wirbelerregte Querschwingungen im Bauzustand wurde mit Spoilern in Form von Gerüstkuben erreicht. Die Windbeanspruchung der frei stehenden hohen Pfeiler war maßgebend für die Bemessung und führte zu hohen Bewehrungsgraden in den Pfählen und Pfeilern. Die Bauausführung hat darauf mit einer optimierten Baulogistik und einer daran angepassten Kletterschalung reagiert, um den Vorlauf gegenüber dem Einschub des Stahlüberbaus zu gewährleisten.
Adjustment for exploratory cut‐off selection in randomized clinical trials with survival endpoint
Authors: Götte Heiko , Kirchner Marietta , Kieser Meinhard 
Published: October 2019

AbstractDefining the target population based on predictive biomarkers plays an important role during clinical development. After establishing a relationship between a biomarker candidate and response to treatment in exploratory phases, a subsequent confirmatory trial ideally involves only subjects with high potential of benefiting from the new compound. In order to identify those subjects in case of a continuous biomarker, a cut‐off is needed. Usually, a cut‐off is chosen that resulted in a subgroup with a large observed treatment effect in an exploratory trial. However, such a data‐driven selection may lead to overoptimistic expectations for the subsequent confirmatory trial. Treatment effect estimates, probability of success, and posterior probabilities are useful measures for deciding whether or not to conduct a confirmatory trial enrolling the biomarker‐defined population. These measures need to be adjusted for selection bias. We extend previously introduced Approximate Bayesian Computation techniques for adjustment of subgroup selection bias to a time‐to‐event setting with cut‐off selection. Challenges in this setting are that treatment effects become time‐dependent and that subsets are defined by the biomarker distribution. Simulation studies show that the proposed method provides adjusted statistical measures which are superior to naïve Maximum Likelihood estimators as well as simple shrinkage estimators.
Development and evaluation of the General Surgery Objective Structured Assessment of Technical Skill (GOSATS)
Authors: Halwani Y , Sachdeva A K , Satterthwaite L , de Montbrun S 
Published: October 2019

Abstract

Background
Technical skill acquisition is important in surgery specialty training. Despite an emphasis on competency-based training, few tools are currently available for direct technical skills assessment at the completion of training. The aim of this study was to develop and validate a simulated technical skill examination for graduating (postgraduate year (PGY)5) general surgery trainees.

Methods
A simulated eight-station, procedure-based general surgery technical skills examination was developed. Board-certified general surgeons blinded to the level of training rated performance of PGY3 and PGY5 trainees by means of validated scoring. Cronbach's α was used to calculate reliability indices, and a conjunctive model to set a pass score with borderline regression methodology. Subkoviak methodology was employed to assess the reliability of the pass–fail decision. The relationship between passing the examination and PGY level was evaluated using χ2 analysis.

Results
Ten PGY3 and nine PGY5 trainees were included. Interstation reliability was 0·66, and inter-rater reliability for three stations was 0·92, 0·97 and 0·76. A pass score of 176·8 of 280 (63·1 per cent) was set. The pass rate for PGY5 trainees was 78 per cent (7 of 9), compared with 30 per cent (3 of 10) for PGY3 trainees. Reliability of the pass–fail decision had an agreement coefficient of 0·88. Graduating trainees were significantly more likely to pass the examination than PGY3 trainees (χ2 = 4·34, P = 0·037).

Conclusion
A summative general surgery technical skills examination was developed with reliability indices within the range needed for high-stakes assessments. Further evaluation is required before the examination can be used in decisions regarding certification.