bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enterococcus faecalis exploits the human fibrinolytic system to drive excess collagenolysis: implications in gut healing and identification of druggable targets
Authors: Jacobson Richard A. , Wienholts Kiedo , Williamson Ashley J. , Gaines Sara , Hyoju Sanjiv , van Goor Harry , Zaborin Alexander , Shogan Benjamin D. , Zaborina Olga , Alverdy John C. 
Published: October 2019

Perforations, anastomotic leak, and subsequent intra-abdominal sepsis are among the most common and feared complications of invasive interventions in the colon and remaining intestinal tract. During physiological healing, tissue protease activity is finely orchestrated to maintain the strength and integrity of the submucosa collagen layer in the wound. We (Shogan, BD et al. Sci Trans Med 7: 286ra68, 2015.) have previously demonstrated in both mice and humans that the commensal microbe Enterococcus faecalis selectively colonizes wounded colonic tissues and disrupts the healing process by amplifying collagenolytic matrix-metalloprotease activity toward excessive degradation. Here, we demonstrate for the first time, to our knowledge, a novel collagenolytic virulence mechanism by which E. faecalis is able to bind and locally activate the human fibrinolytic protease plasminogen (PLG), a protein present in high concentrations in healing colonic tissue. E. faecalis-mediated PLG activation leads to supraphysiological collagen degradation; in this study, we demonstrate this concept both in vitro and in vivo. This pathoadaptive response can be mitigated with the PLG inhibitor tranexamic acid (TXA) in a fashion that prevents clinically significant complications in validated murine models of both E. faecalis- and Pseudomonas aeruginosa-mediated colonic perforation. TXA has a proven clinical safety record and is Food and Drug Administration approved for topical application in invasive procedures, albeit for the prevention of bleeding rather than infection. As such, the novel pharmacological effect described in this study may be translatable to clinical trials for the prevention of infectious complications in colonic healing. NEW & NOTEWORTHY This paper presents a novel mechanism for virulence in a commensal gut microbe that exploits the human fibrinolytic system and its principle protease, plasminogen. This mechanism is targetable by safe and effective nonantibiotic small molecules for the prevention of infectious complications in the healing gut.
BDNF downregulates β-adrenergic receptor-mediated hypotensive mechanisms in the paraventricular nucleus of the hypothalamus
Authors: Thorsdottir Daniella , Cruickshank Nicholas C. , Einwag Zachary , Hennig Grant W. , Erdos Benedek 
Published: October 2019

Brain-derived neurotrophic factor (BDNF) is upregulated in the paraventricular nucleus of the hypothalamus (PVN) in response to hypertensive stimuli such as stress and hyperosmolality, and BDNF acting in the PVN plays a key role in elevating sympathetic activity and blood pressure. However, downstream mechanisms mediating these effects remain unclear. We tested the hypothesis that BDNF increases blood pressure, in part by diminishing inhibitory hypotensive input from nucleus of the solitary tract (NTS) catecholaminergic neurons projecting to the PVN. Male Sprague-Dawley rats received bilateral PVN injections of viral vectors expressing either green fluorescent protein (GFP) or BDNF and bilateral NTS injections of vehicle or anti-dopamine-β-hydroxylase-conjugated saporin (DSAP), a neurotoxin that selectively lesions noradrenergic and adrenergic neurons. BDNF overexpression in the PVN without NTS lesioning significantly increased mean arterial pressure (MAP) in awake animals by 18.7 ± 1.8 mmHg. DSAP treatment also increased MAP in the GFP group, by 9.8 ± 3.2 mmHg, but failed to affect MAP in the BDNF group, indicating a BDNF-induced loss of NTS catecholaminergic hypotensive effects. In addition, in α-chloralose-urethane-anesthetized rats, hypotensive responses to PVN injections of the β-adrenergic agonist isoprenaline were significantly attenuated by BDNF overexpression, whereas PVN injections of phenylephrine had no effect on blood pressure. BDNF treatment was also found to significantly reduce β1-adrenergic receptor mRNA expression in the PVN, whereas expression of other adrenergic receptors was unaffected. In summary, increased BDNF expression in the PVN elevates blood pressure, in part by downregulating β-receptor signaling and diminishing hypotensive catecholaminergic input from the NTS to the PVN. NEW & NOTEWORTHY We have shown that BDNF, a key hypothalamic regulator of blood pressure, disrupts catecholaminergic signaling between the NTS and the PVN by reducing the responsiveness of PVN neurons to inhibitory hypotensive β-adrenergic input from the NTS. This may be occurring partly via BDNF-mediated downregulation of β1-adrenergic receptor expression in the PVN and results in an increase in blood pressure.
Simulation-Based Mastery Learning Improves Patient and Caregiver Ventricular Assist Device Self-Care Skills
Authors: Barsuk Jeffrey H. , Wilcox Jane E. , Cohen Elaine R. , Harap Rebecca S. , Shanklin Kerry B. , Grady Kathleen L. , Kim Jane S. , Nonog Gretchen P. , Schulze Lauren E. , Jirak Alison M. , Wayne Diane B. , Cameron Kenzie A. 
Published: October 2019

Background:
No recognized standards exist for teaching patients and their caregivers ventricular assist device (VAD) self-care skills. We compared the effectiveness of a VAD simulation-based mastery learning (SBML) self-care training curriculum with usual VAD self-care training.

Methods and Results:

VAD patients and their caregivers were randomized to SBML or usual training during their implant hospitalization. The SBML group completed a pretest on 3 VAD self-care skills (controller, power source, and dressing change), then viewed videos and participated in deliberate practice on a simulator. SBML participants took a posttest and were required to meet or exceed a minimum passing standard for each of the skills. The usual training group completed the existing institutional VAD self-care teaching protocol. Before hospital discharge, the SBML and usual training groups took the same 3 VAD self-care skills tests. We compared demographic and clinical information, self-confidence, total participant training time, and skills performance between groups. Forty participants completed the study in each group. There were no differences in demographic and clinical information, self-confidence, or training time between groups. More participants in the SBML group met the minimum passing standard compared with the usual training group for controller (37/40 [93%] versus 25/40 [63%];
P
=0.001), power source (36/40 [90%] versus 9/40 [23%];
P
<0.001), and dressing change skills (19/20 [95%] versus 0/20;
P
<0.001).

Conclusions:
SBML provided superior VAD self-care skills learning outcomes compared with usual training. This study has important implications for patients due to the morbidity and mortality associated with improper VAD self-care.

Clinical Trial Registration:

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT03073005.
The Abramtsevo Artistic Circle and the Search for a National Landscape
Authors: Hilton Alison 
Published: October 2019

Abstract
The strong national voice at Abramtsevo, based on a sense of harmony among native landscapes, religious and folk life, and estate culture was intrinsic to Slavic revival movements of the late nineteenth century. The estate and its surroundings were settings for Russian-themed paintings and inspired artists to seek and express a Russian “spirit of nature.” The search for a national landscape was connected with literary and intellectual culture fostered at Abramtsevo and neighboring estates, and with the presence of religious centers in the area. Local topography and collaboration among the Abramtsevo artists in the 1880s led to new ideas about a national landscape as artists ranged further afield in the next decade. Landscapes of mood and decorative works based on natural forms shifted the role of landscape from concrete subject to a source for formal experimentation.
Russia in Rome
Authors: Tonini Lucia 
Published: October 2019

Abstract
The presence of thirty-three Russian head-dresses, as well as other historical objects, in the collection of the American diplomat, George Wurts, and his wife, Henrietta Tower, is an uncommon example of collecting Russian folk objects abroad, and testifies to a universality of taste in international collecting during the late nineteenth century. The head-dress collection is part of a larger collection of around 4,000 pieces dating from antiquity to the early twentieth century, which was assembled at the Palazzo Antici Mattei and the Villa Sciarra in Rome between the end of the nineteenth and the beginning of the twentieth century. Wurts and Tower had a particular interest in arts and crafts, which was enabled by Wurts’s career as a diplomat and secretary at the American mission in St. Petersburg for a period of ten years (1882-93).
This article describes the key characteristics of the Wurts-Tower collection of folk objects, the circumstances of its formation, and its relation to the tendencies of taste during that time. It also testifies to the transformation of the kokoshnik in the eyes of collectors and viewers from a popular costume to a fashion accessory that was linked to a past world.
Invisible Women
Authors: Hardiman Louise 
Published: October 2019

Abstract
Maria Vasilievna Iakunchikova designed three works of applied art and craft in a Neo-Russian style for the Russian section of the Paris “Exposition Universelle” of 1900—a wooden dresser, a toy village in carved wood, and a large embroidered panel. Yet, so far as the official record is concerned, Iakunchikova’s participation in the exhibition is occluded. Her name does not appear in the catalogue, for it was the producers, rather than the designers, who were credited for her works. Indeed, her presence might have been entirely unknown, were it not for several reports of the Russian display in the periodical press by her friend Netta Peacock, a British writer living in Paris. The invisibility of the designer in this instance was not a matter of gender, but it had consequences for women artists. In general, women were marginalized in the mainstream of the nineteenth-century Russian art world—whether at the Academy of Arts or in prominent groups such as the Peredvizhniki—and, as a result, enjoyed fewer opportunities at the Exposition. But the Neo-national movement, linked closely with the revival of applied art and the promotion of kustar industries, was one in which women’s art had space to flourish. And, in the so-called village russe at the Exposition, which featured a display of kustar art, by far the larger contribution was made by women, both as promoters and as artists. In this article, I examine Iakunchikova’s contribution to the Exposition within a broader context of female artistic activity, and the significance of the Russian kustar pavilion for a gendered history of nineteenth-century art.
Artists at Play
Authors: Winestein Anna 
Published: October 2019

Abstract
The exhibition of Russian folk art at the Paris “Salon d’Automne” of 1913 has been generally overlooked in scholarship on folk art, overshadowed by the “All-Russian Kustar Exhibitions” and the Moscow avant-garde gallery shows of the same year. This article examines the contributions of its curator, Natalia Erenburg, and the project’s instigator, Iakov Tugendkhold, who wrote the catalogue essay and headed the committee—both of whom were artists who became critics, historians, and collectors. The article elucidates the show’s rationale and selection of exhibits, the critical response to it and its legacy. It also discusses the artistic circles of Russian Paris in which the project originated, particularly the Académie russe. Finally, it examines the project in the context of earlier efforts to present Russian folk art in Paris, and shows how it—and Russian folk art as a source and object of collecting and display—brought together artists, collectors, and scholars from the ranks of the Mir iskusstva [World of Art] group, as well as the younger avant-gardists, and allowed them to engage Parisian and European audiences with their own ideas and artworks.
Stronger Increase in the Frequency of Extreme Convective than Extreme Warm El Niño Events under Greenhouse Warming
Authors: Wang Guojian , Cai Wenju , Santoso Agus 
Published: October 2019

AbstractSince 1979, three extreme El Niño events occurred, in 1982/83, 1997/98, and 2015/16, with pronounced impacts that disrupted global weather patterns, agriculture, fisheries, and ecosystems. Although all three episodes are referred to as strong equatorial eastern Pacific (EP) El Niño events, the 2015/16 event is considered a mixed regime of both EP and central Pacific (CP) El Niño. During such extreme events, sea surface temperature (SST) anomalies peak over the EP region, hereafter referred to as an extreme warm El Niño (ExtWarmEN) event. Simultaneously, the intertropical convergence zone (ITCZ) moves southward to the usually dry and cold Niño-3 region, resulting in dramatic rainfall increases to more than 5 mm day−1 averaged over boreal winter, referred to as an extreme convective El Niño (ExtConEN) event. However, in climate models from phase 5 of the Coupled Model Intercomparison Project (CMIP5) that are able to simulate both types of events, ExtConEN events are found not to always coincide with ExtWarmEN events and the disassociation becomes more distinct under greenhouse warming when the increased frequency of ExtConEN events is notably larger than that of ExtWarmEN events. The disassociation highlights the role of eastward migration of western Pacific convection and equatorward shift of the South Pacific convergence zone associated with the faster warming over the EP region as a result of greenhouse warming.
Measuring User Experience With 3, 5, 7, or 11 Points
Authors: Lewis James R. 
Published: October 2019

Objective To assess versions of the shorter form variant of Usability Metric for User Experience (UMUX-LITE) questionnaire differing in the number of response options for the items (3, 5, 7, and 11). Background The UMUX-LITE is an efficient (two-item) standardized questionnaire that measures perceived usability. A growing body of evidence shows it closely corresponds to one of the most widely used standardized usability questionnaires, the System Usability Scale (SUS), with regard to both correlation and magnitude of concurrently collected means. Although the “standard” version of the UMUX-LITE uses items with seven response options, there is some variance in practice. Method Members of a corporate user experience panel ( n = 242) completed surveys rating a recent Web site experience with the SUS and UMUX-LITE, also providing ratings of overall experience and likelihood-to-recommend. Results Scale reliabilities were acceptable (coefficient α >.70) with the exception of UMUX-LITE with three response options. All UMUX-LITE correlations with SUS, overall experience, and likelihood-to-recommend were highly significant. For likelihood-to-recommend, there was a significant difference in the magnitude of correlations, with 11 response options higher than three. Although some statistically significant differences were observed in correspondence between SUS and UMUX-LITE scores, these did not seem to translate to practically significant differences. Conclusion The number of UMUX-LITE response options does not matter much, especially in practice. Because the version with three response options showed some weakness with regard to reliability and correlation with likelihood-to-recommend, practitioners should avoid it. Application Unless there is a strong reason to do otherwise, use the “standard” version with seven response options.
Intradialytic exercise with blood flow restriction is more effective than conventional exercise in improving walking endurance in hemodialysis patients: a randomized controlled trial
Authors: Cardoso Rodrigo Kohn , Araujo Aline Machado , Del Vechio Fabrício Boscolo , Bohlke Maristela , Barcellos Franklin Correa , Oses Jean Pierre , de Freitas Matheus Pintanel , Rombaldi Airton José 
Published: October 2019

Objective: This study aims to compare the effect of intradialytic aerobic exercise with blood flow restriction, without blood flow restriction (conventional) and no exercise (control) on muscle strength and walking endurance among chronic kidney disease patients. Design: Open label and parallel group randomized controlled trial. Subjects: Adult patients with chronic kidney disease on hemodialysis. Intervention: A 12-week intradialytic training with or without blood flow restriction compared with a control group. Main measures: Strength and walking endurance were measured using thoracolumbar dynamometry and a 6-minute walk test, respectively, before and after training. Results: A total of 66 patients were randomized into three groups: blood flow restriction group ( n = 22), conventional exercise group ( n = 22) and control group ( n = 22). There were seven dropouts, and 59 patients were included in the analysis. There was a significant increase in the 6-minute walking distance in the blood flow restriction group (from 412.7 (115.9) to 483.0 (131.0) m, P = 0.007) in comparison with the conventional exercise group (from 426.79 (115.00) to 433.2 (120.42) m, not significant) and the control group (from 428.4 (108.1) to 417.3 (100.2) m, not significant). The change in the walking distance over time was significantly different among groups (intervention group/time, P = 0.02). The simple effects test found a significant time effect only in the blood flow restriction group. There was no significant difference in strength change between the groups. Conclusion: Among chronic kidney disease patients, intradialytic exercise of low/moderate intensity with blood flow restriction was more effective in improving walking endurance than conventional exercise or no exercise.
miR-98 reduces endothelial dysfunction by protecting blood–brain barrier (BBB) and improves neurological outcomes in mouse ischemia/reperfusion stroke model
Authors: Bernstein David L , Zuluaga-Ramirez Viviana , Gajghate Sachin , Reichenbach Nancy L , Polyak Boris , Persidsky Yuri , Rom Slava 
Published: October 2019

Most neurological diseases, including stroke, lead to some degree of blood–brain barrier (BBB) dysfunction. A significant portion of BBB injury is caused by inflammation, due to pro-inflammatory factors produced in the brain, and by leukocyte engagement of the brain endothelium. Recently, microRNAs (miRNAs) have appeared as major regulators of inflammation-induced changes to gene expression in the microvascular endothelial cells (BMVEC) that comprise the BBB. However, miRNAs’ role during cerebral ischemia/reperfusion is still underexplored. Endothelial levels of miR-98 were significantly altered following ischemia/reperfusion insults, both in vivo and in vitro, transient middle cerebral artery occlusion (tMCAO), and oxygen–glucose deprivation (OGD), respectively. Overexpression of miR-98 reduced the mouse’s infarct size after tMCAO. Further, miR-98 lessened infiltration of proinflammatory Ly6CHI leukocytes into the brain following stroke and diminished the prevalence of M1 (activated) microglia within the impacted area. miR-98 attenuated BBB permeability, as demonstrated by changes to fluorescently-labeled dextran penetration in vivo and improved transendothelial electrical resistance (TEER) in vitro. Treatment with miR-98 improved significantly the locomotor impairment. Our study provides identification and functional assessment of miRNAs in brain endothelium and lays the groundwork for improving therapeutic approaches for patients suffering from ischemic attacks.
Positive Effect of Platelet-Rich Plasma on Pain in Plantar Fasciitis: A Double-Blind Multicenter Randomized Controlled Trial
Authors: Peerbooms Joost C. , Lodder Paul , den Oudsten Brenda L. , Doorgeest Kamiel , Schuller Hans M. , Gosens Taco 
Published: October 2019

Background: When nonoperative treatment for chronic plantar fasciitis fails, often a corticosteroid injection is given. Corticosteroid injection gives temporary pain reduction but no healing. Platelet-rich plasma (PRP) has proven to be a safe therapeutic option in the treatment of tendon, muscle, bone, and cartilage injuries. Purpose: To determine the effectiveness of PRP as compared with corticosteroid injections for chronic plantar fasciitis. Study Design: Randomized controlled trial; Level of evidence, 1. Methods: Patients with chronic plantar fasciitis were allocated to have steroid injection or PRP. The primary outcome measure was the Foot Function Index (FFI) Pain score. Secondary outcome measures were function, as scored by the FFI Activity, FFI Disability, and American Orthopaedic Foot & Ankle Society, and quality of life, as scored with the short version of the World Health Organization Quality of Life (WHOQOL-BREF). All outcomes were measured at baseline and at 4, 12, and 26 weeks and 1 year after the procedure. Results: Of the 115 patients, 63 were allocated to the PRP group, of which 46 (73%) completed the study, and 52 were allocated to the control group (corticosteroid injection), of which 36 (69%) completed the study. In the control group, FFI Pain scores decreased quickly and then remained stable during follow-up. In the PRP group, FFI Pain reduction was more modest but reached a lower point after 12 months than the control group. After adjusting for baseline differences, the PRP group showed significantly lower pain scores at the 1-year follow-up than the control group (mean difference, 14.4; 95% CI, 3.2-25.6). The number of patients with at least 25% improvement (FFI Pain score) between baseline and 12-month follow-up differed significantly between the groups. Of the 46 patients in the PRP group, 39 (84.4%) improved at least 25%, while only 20 (55.6%) of the 36 in the control group showed such an improvement ( P = .003). The PRP group showed significantly lower FFI Disability scores than the control group (mean difference, 12.0; 95% CI, 2.3-21.6). Conclusion: Treatment of patients with chronic plantar fasciitis with PRP seems to reduce pain and increase function more as compared with the effect of corticosteroid injection. Registration: NCT00758641 (ClinicalTrials.gov identifier).