bluuebunny/crossref_metadata_2025_split huggingface dataset:
A stereoscopic reading of Rosario Ferré’s “El cuento envenenado” and “The Poisoned Story”
Authors: Ayuso Mónica G. 
Published: April 2019

Abstract
Rosario Ferré has been a self-translator since the mid-1980s. For just as long, criticism of her work has been
snarled in essentialist arguments that assume that language embodies the values of the culture from which it derives and that
words transmit an essence regardless of context. Following this logic, English and Spanish are systems in opposition. This essay
compares “El cuento envenenado” (1986) to Ferré’s self-translation, “The Poisoned Story” (1991) in order to recast her as a bilingual writer. Using Marilyn Gaddis Rose’s concept of stereoscopic reading, the
essay places two autonomous but interrelated texts in a theory-informed relationship that renders them as one textual space and
captures the creative interliminality of a bilingual writer. This reading traces Ferré’s evolution as a writer from the late
seventies to the nineties and beyond, when she envisioned a short story of greater inscrutability and resistance that reflected
the legacy of colonialism within a Puerto Rican household.
Identifying translation problems in English-Chinese sight translation
Authors: Su Wenchao , Li Defeng 
Published: April 2019

Abstract
Translation problems have received considerable attention among translation process researchers and different
research methods have been used to identify them. Findings are sometimes inconsistent, and as these studies have mainly studied
translation between European languages, little research has been conducted to explore the issue concerning non-European languages.
To fill this gap, the present study investigates problem triggers in English-Chinese sight translation in both directions (L1 and
L2 translation). using eye-tracking data (Dragsted 2012). Results suggest that the type
and number of translation problems encountered by the translators are different in L1 and L2 sight translation and that
language-pair specificity is at play during the process, indicated by two identified Chinese-specific problem triggers, namely,
back-sloping comma and head-final noun phrase.
Promotion of alleles by genome engineering.
Authors: Cole J. B. 
Published: April 2019

Abstract
Traditional genetic selection programmes based on pedigree and performance information have been used to improve livestock populations for decades. The introduction of high-density single nucleotide polymorphism genotyping about 10 years ago supported increased rates of gain through more accurate prediction of genetic merit earlier in life. Recent continued technological advances enable the routine use of genetic engineering and gene editing tools in livestock research and, increasingly, production systems. Livestock geneticists have responded by proposing new breeding schemes that combine traditional selection methodology with these new tools to substantially increase rates of genetic gain while reducing harmful effects due to decreased heterozygosity. Genetic improvement strategies based on gene drives have the potential further increase rates of gain but pose risks that may not be acceptable to the public. Intense debate about the use of these technologies in the animal food chain is being driven by regulatory agencies and consumer advocates, and it is not clear if genetically modified animals will be acceptable to consumers. This review focuses on the application of genetic engineering and genome engineering tools to livestock population improvement through the management of genetic load and the promotion of desirable alleles in the population associated with both monogenic and polygenic traits. Limitations of the current technology, such as limited knowledge of true causal variants, are discussed, as are regulatory and consumer acceptance issues.
A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing
Authors: Ristola Mervi , Sukki Lassi , Azevedo Maria Manuela , Seixas Ana Isabel , Relvas João Bettencourt , Narkilahti Susanna , Kallio Pasi 
Published: April 2019

Abstract
Microfluidics devices for co-culturing neurons and oligodendrocytes represent an important in vitro research tool to decipher myelination mechanisms in health and disease and in the identification of novel treatments for myelin diseases. In reported devices using primary rodent cells, the spontaneous formation of myelin sheaths has been challenging and random orientation of neurites impede the analysis of myelination. Furthermore, fabrication methods for devices show limitations, highlighting the need for novel in vitro cell-based myelination models. In the present study, we describe a compartmentalized cell culture device targeted for neuron-oligodendrocyte co-culturing and myelination studies. In the device, neurites from primary rat dorsal root ganglion (DRG) neurons were capable of forming aligned dense networks in a specific compartment that was physically isolated from neuronal somas. Co-culture of rat DRG neurons and oligodendrocytes, a well-known model to study myelination in vitro, led to interactions between oligodendrocytes and neurites in the device, and the deposition of myelin segments in an aligned distribution was spontaneously formed. For the fabrication of the device, we present a new method that produces polydimethylsiloxane (PDMS)—based devices possessing an open compartment design. The proposed fabrication method takes advantage of an SU-8 photolithography process and 3D printing for mould fabrication. Both the microscale and macroscale features are replicated from the same mould, allowing devices to be produced with high precision and repeatability. The proposed device is applicable for long-term cell culturing, live-cell imaging, and by enhancing aligned myelin distribution, it is a promising tool for experimental setups that address diverse biological questions in the field of myelin research.
Tuning of impurity-bound interlayer complexes in a van der Waals heterobilayer
Authors: Vialla Fabien , Danovich Mark , Ruiz-Tijerina David A , Massicotte Mathieu , Schmidt Peter , Taniguchi Takashi , Watanabe Kenji , Hunt Ryan J , Szyniszewski Marcin , Drummond Neil D , Pedersen Thomas G , Fal’ko Vladimir I , Koppens Frank H L 
Published: April 2019

Abstract
Due to their unique 2D nature, charge carriers in semiconducting transition metal dichalcogenides (TMDs) exhibit strong unscreened Coulomb interactions and sensitivity to defects and impurities. The versatility of van der Waals layer stacking allows spatially separating electrons and holes between different TMD layers with staggered band structure, yielding interlayer few-body excitonic complexes whose nature is still debated. Here we combine quantum Monte Carlo calculations with spectrally and temporally resolved photoluminescence (PL) measurements on a top- and bottom-gated MoSe2/WSe2 heterostructure, and identify the emitters as impurity-bound interlayer excitonic complexes. Using independent electrostatic control of doping and out-of-plane electric field, we demonstrate control of the relative populations of neutral and charged complexes, their emission energies on a scale larger than their linewidth, and an increase of their lifetime into the microsecond regime. This work unveils new physics of confined carriers and is key to the development of novel optoelectronics applications.
Optimal control of hybrid optomechanical systems for generating non-classical states of mechanical motion
Authors: Bergholm Ville , Wieczorek Witlef , Schulte-Herbrüggen Thomas , Keyl Michael 
Published: April 2019

Abstract
Cavity optomechanical systems are one of the leading experimental platforms for controlling mechanical motion in the quantum regime. We exemplify that the control over cavity optomechanical systems greatly increases by coupling the cavity also to a two-level system, thereby creating a hybrid optomechanical system. If the two-level system can be driven largely independently of the cavity, we show that the nonlinearity thus introduced enables us to steer the extended system to non-classical target states of the mechanical oscillator with Wigner functions exhibiting significant negative regions. We illustrate how to use optimal control techniques beyond the linear regime to drive the hybrid system from the near ground state into a Fock target state of the mechanical oscillator. We base our numerical optimization on realistic experimental parameters for exemplifying how optimal control enables the preparation of decidedly non-classical target states, where naive control schemes fail. Our results thus pave the way for applying the toolbox of optimal control in hybrid optomechanical systems for generating non-classical mechanical states.
Association of Social Adversity with Comorbid Diabetes and Depression Symptoms in the Hispanic Community Health Study/Study of Latinos Sociocultural Ancillary Study: A Syndemic Framework
Authors: McCurley Jessica L , Gutierrez Angela P , Bravin Julia I , Schneiderman Neil , Reina Samantha A , Khambaty Tasneem , Castañeda Sheila F , Smoller Sylvia , Daviglus Martha L , O’Brien Matthew J , Carnethon Mercedes R , Isasi Carmen R , Perreira Krista M , Talavera Greg A , Yang Mingan , Gallo Linda C 
Published: April 2019

AbstractBackgroundU.S. Hispanics/Latinos experience high lifetime risk for Type 2 diabetes and concurrent psychological depression. This comorbidity is associated with poorer self-management, worse disease outcomes, and higher mortality. Syndemic theory is a novel social epidemiological framework that emphasizes the role of economic and social adversity in promoting disease comorbidity and health disparities.PurposeInformed by the syndemic framework, this study explored associations of socioeconomic and psychosocial adversity (low income/education, trauma history, adverse childhood experiences, ethnic discrimination, neighborhood problems [e.g., violence]) with comorbidity of diabetes and depression symptoms in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) and Sociocultural Ancillary Study.MethodsParticipants were 5,247 Latino adults, aged 18–74, enrolled in four U.S. cities from 2008 to 2011. Participants completed a baseline physical exam and measures of depression symptoms and psychosocial adversity. Multinomial logistic regression analyses were conducted to examine associations of adversity variables with comorbid diabetes and high depression symptoms.ResultsHousehold income below $30,000/year was associated with higher odds of diabetes/depression comorbidity (odds ratio [OR] = 4.61; 95% confidence interval [CI]: 2.89, 7.33) compared to having neither condition, as was each standard deviation increase in adverse childhood experiences (OR = 1.41; 95% CI: 1.16, 1.71), ethnic discrimination (OR = 1.23; 95% CI: 1.01, 1.50), and neighborhood problems (OR = 1.53; 95% CI: 1.30, 1.80).ConclusionLow household income, adverse childhood experiences, ethnic discrimination, and neighborhood problems are related to comorbid diabetes and depression in U.S. Latinos. Future studies should explore these relationships longitudinally.
A process-based model for leaf development and growth in hardneck garlic (Allium sativum)
Authors: Hsiao Jennifer , Yun Kyungdahm , Moon Kyung Hwan , Kim Soo-Hyung 
Published: April 2019

Abstract

Background and aims
Phenology and morphology are two major aspects of crop growth models. A new process-based model built for hardneck garlic (Allium sativum) is presented, focusing on phenology and morphology processes and how they translate to whole-plant growth. The tight coupling between the two processes and their dynamic changes throughout the growing season were captured while incorporating storage effects and reproductive aspects unique to bulbous crops.

Methods
Non-linear temperature dependences of leaf development were integrated into the model and dynamically coupled with changes in leaf growth throughout the growing season. Bulb storage effects on leaf development and photoperiod effects on the vegetative-to-reproductive transition were also incorporated. The model was parameterized with data from a set of experiments and the literature, while its performance was tested with additional observations that had not been used for parameterization under a range of environmental conditions, management practices and cultivar choices.

Key Results
The model successfully captured the dynamic nature of leaf development and growth in garlic plants throughout the growing season. It simulated with reasonable accuracy the timing of leaf initiation, maturation and senescence, as well as changes in green leaf area over time. Most parameters were relatively stable across cultivars, and parameter sensitivity tests revealed the importance of bulb storage effects.

Conclusions
The model embodies a novel approach to capture the phenology and morphology of garlic under a range of environments, genotypes and management practices. The process-oriented nature of the model and inclusion of storage effects set the foundation for bulbous crop growth simulations, allowing the understanding and discovery of key processes that coordinate and integrate the dynamics of growth and development from organ to whole plant, with implications for crop improvement programmes while opening opportunities for modelling other bulbous crops.
SolidBin: improving metagenome binning with semi-supervised normalized cut
Authors: Wang Ziye , Wang Zhengyang , Lu Yang Young , Sun Fengzhu , Zhu Shanfeng 
Published: April 2019

Abstract

Motivation
Metagenomic contig binning is an important computational problem in metagenomic research, which aims to cluster contigs from the same genome into the same group. Unlike classical clustering problem, contig binning can utilize known relationships among some of the contigs or the taxonomic identity of some contigs. However, the current state-of-the-art contig binning methods do not make full use of the additional biological information except the coverage and sequence composition of the contigs.

Results
We developed a novel contig binning method, Semi-supervised Spectral Normalized Cut for Binning (SolidBin), based on semi-supervised spectral clustering. Using sequence feature similarity and/or additional biological information, such as the reliable taxonomy assignments of some contigs, SolidBin constructs two types of prior information: must-link and cannot-link constraints. Must-link constraints mean that the pair of contigs should be clustered into the same group, while cannot-link constraints mean that the pair of contigs should be clustered in different groups. These constraints are then integrated into a classical spectral clustering approach, normalized cut, for improved contig binning. The performance of SolidBin is compared with five state-of-the-art genome binners, CONCOCT, COCACOLA, MaxBin, MetaBAT and BMC3C on five next-generation sequencing benchmark datasets including simulated multi- and single-sample datasets and real multi-sample datasets. The experimental results show that, SolidBin has achieved the best performance in terms of F-score, Adjusted Rand Index and Normalized Mutual Information, especially while using the real datasets and the single-sample dataset.

Availability and implementation
https://github.com/sufforest/SolidBin.

Supplementary information
Supplementary data are available at Bioinformatics online.
Possible reproductive benefits to female Carpetan rock lizards of pre-sensory bias towards chemical signals
Authors: Rodríguez-Ruiz Gonzalo , López Pilar , Martín José 
Published: April 2019

Abstract
Female Carpetan rock lizards (Iberolacerta cyreni) might assess the quality of males from their chemical signals. Females select areas scent marked by males with secretions containing high proportions of provitamin D to ensure mating with high-quality males. However, an alternative explanation might be that females are not choosing a mate, but that females have a pre-sensory bias for chemical cues of vitamin D in the food and are, in fact, assessing habitat quality to obtain direct benefits of increasing the intake of vitamin D. We evaluated experimentally the possible benefits of a nutritional supplement of provitamin D or vitamin D in pregnant females for their clutches and offspring. However, we did not find large differences between treatments, except in the lower body condition of juveniles of mothers supplemented with provitamin D. We also tested the chemosensory interest of females in the scent of males to study the existence of a sensory bias; this response decreased when the amount of dietary vitamin D exceeded their needs. The results suggest that there are no reproductive benefits in the intake of additional vitamin D and that the potential sensory bias to scents of males could be related to the physiological needs of the females.
GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice†
Authors: Shiromoto Yusuke , Kuramochi-Miyagawa Satomi , Nagamori Ippei , Chuma Shinichiro , Arakawa Tatsuhiko , Nishimura Toru , Hasuwa Hidetoshi , Tachibana Taro , Ikawa Masahito , Nakano Toru 
Published: April 2019

Abstract
PIWI-interacting RNAs (piRNAs), a subclass of germ cell-specific noncoding small RNAs, are essential for de novo DNA methylation of retrotransposon genes in embryonic testes. PIWIL2/MILI, one of three mouse PIWI family members, is indispensable for piRNA production, DNA methylation of retrotransposons presumably via piRNA, and normal spermatogenesis. In vitro analysis using germline stem cells (GS cells) revealed that glycerol-3-phosphate acyltransferase 2 (GPAT2), which is a mitochondrial outer membrane protein involved in generation of lysophosphatidic acid (LPA) and highly expressed in testes, plays important roles in spermatogenesis. Namely, GPAT2 binds to PIWIL2 and is closely involved in the biogenesis of piRNAs; this process is independent of its enzymatic activity on LPA. However, GS cells recapitulate only a limited phase of spermatogenesis and the biological functions of GPAT2 remain largely unknown. In this study, we generated GPAT2-deficient mice and conducted comprehensive analyses. The deficient mice showed defective piRNA production and subsequent de-silencing of IAP and Line-1 retrotransposons in fetal testes. In addition, apoptosis of pachytene spermatocytes was observed. These abnormalities were all common to the phenotype of PIWIL2-deficient mice, in which piRNA production was impaired. GPAT2-deficient mice exhibited apoptosis in spermatogonia at the neonatal stage, which was not observed in PIWIL2-deficient mice. These data show that GPAT2 plays a critical role in preventing apoptosis in spermatogonia.
Validated RP-HPLC Method for Simultaneous Determination of Ribavirin, Sofosbuvir and Daclatasvir in Human Plasma: A Treatment Protocol Administered to HCV Patients in Egypt
Authors: Youssef Aya A , Magdy N , Hussein Lobna A , El-Kosasy A M 
Published: April 2019

Abstract
Egypt has the highest prevalence of hepatitis C virus (HCV) in the world thus it launched a national program for eliminating HCV aiming to treat 300,000 HCV patients per year. Three anti-HCV co-administered drugs; ribavirin (RBV), sofosbuvir (SF) daclatasvir (DAC) were simultaneously determined in human plasma by a validated, simple and sensitive RP-HPLC method using propyl paraben as an internal standard. Liquid–liquid extraction using ethyl acetate was used for samples extraction. Chromatographic separation was achieved on Scharlau® C18 column (250 × 4.6 mm2, 5 μm). Gradient elution was employed with a mobile phase mixture of water and acetonitrile at a flow rate 1 mL/min. UV detection using photodiode array detector was carried out at 207, 260 and 312 nm for RBV, SF and DAC, respectively. Method validation was performed according to the FDA guidelines for bioanalytical method validation. The calibration curves were linear over the ranges (0.5–80, 0.1–40 and 0.5–80 μg/mL) with average recoveries (100.64–108.28%, 98.48–105.91% and 97.68–101.38%) for RBV, SF and DAC, respectively. The intra-day and inter-day precision and accuracy results were within the acceptable limits. Stability assays revealed that the three studied analytes were stable during sample storage, preparation and injection. The method can be successfully applied in routine analysis of plasma of HCV patients treated with this combination therapy which aids in therapeutic drug monitoring and patients’ follow-up especially in Egypt and other developing countries fighting HCV.
Low Reserve Prices in Auctions*
Authors: Hu Audrey , Matthews Steven A , Zou Liang 
Published: April 2019

AbstractA standard result in auction theory is that a seller’s profit-maximising reserve price is no less than his own value for the good. In practice, however, reserve prices often appear to be less than sellers’ values. This article revisits the theory of optimal reserves in the context of second-price auctions. The main result is that an optimal reserve is less than the seller’s value if the bidders are sufficiently risk averse and if their values are sufficiently interdependent. The resulting outcome may approximate that of an auction without reserve, i.e., an absolute auction.
Protease-activated receptor 2 deficiency mediates cardiac fibrosis and diastolic dysfunction
Authors: Friebel Julian , Weithauser Alice , Witkowski Marco , Rauch Bernhard H , Savvatis Konstantinos , Dörner Andrea , Tabaraie Termeh , Kasner Mario , Moos Verena , Bösel Diana , Gotthardt Michael , Radke Michael H , Wegner Max , Bobbert Peter , Lassner Dirk , Tschöpe Carsten , Schutheiss Heinz-Peter , Felix Stephan B , Landmesser Ulf , Rauch Ursula 
Published: April 2019

Abstract

Aims
Heart failure with preserved ejection fraction (HFpEF) and pathological cardiac aging share a complex pathophysiology, including extracellular matrix remodelling (EMR). Protease-activated receptor 2 (PAR2) deficiency is associated with EMR. The roles of PAR1 and PAR2 have not been studied in HFpEF, age-dependent cardiac fibrosis, or diastolic dysfunction (DD).

Methods and results
Evaluation of endomyocardial biopsies from patients with HFpEF (n = 14) revealed that a reduced cardiac PAR2 expression was associated with aggravated DD and increased myocardial fibrosis (r = −0.7336, P = 0.0028). In line, 1-year-old PAR2-knockout (PAR2ko) mice suffered from DD with preserved systolic function, associated with an increased age-dependent α-smooth muscle actin expression, collagen deposition (1.7-fold increase, P = 0.0003), lysyl oxidase activity, collagen cross-linking (2.2-fold increase, P = 0.0008), endothelial activation, and inflammation. In the absence of PAR2, the receptor-regulating protein caveolin-1 was down-regulated, contributing to an augmented profibrotic PAR1 and transforming growth factor beta (TGF-β)-dependent signalling. This enhanced TGF-β/PAR1 signalling caused N-proteinase (ADAMTS3) and C-proteinase (BMP1)-related increased collagen I production from cardiac fibroblasts (CFs). PAR2 overexpression in PAR2ko CFs reversed these effects. The treatment with the PAR1 antagonist, vorapaxar, reduced cardiac fibrosis by 44% (P = 0.03) and reduced inflammation in a metabolic disease model (apolipoprotein E-ko mice). Patients with HFpEF with upstream PAR inhibition via FXa inhibitors (n = 40) also exhibited reduced circulating markers of fibrosis and DD compared with patients treated with vitamin K antagonists (n = 20).

Conclusions
Protease-activated receptor 2 is an important regulator of profibrotic PAR1 and TGF-β signalling in the heart. Modulation of the FXa/FIIa-PAR1/PAR2/TGF-β-axis might be a promising therapeutic approach to reduce HFpEF.