bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paenarthrobacter
Authors: Busse Hans‐Jürgen , Wieser Monika 
Published: June 2018

Paen.ar.thro.bac'ter. L. adv.paene, nearly, almost; N.L. masc. n.Arthrobacter, a bacterial genus name; N.L. masc. n.Paenarthrobacter, almostArthrobacter.Actinobacteria / Actinobacteria / Micrococcales / Micrococcaceae / PaenarthrobacterThe genusPaenarthrobactercontains the speciesPaenarthrobacter aurescens, Paenarthrobacter histidinolovorans, Paenarthrobacter ilicis, Paenarthrobacter nicotinovorans, Paenarthrobacter nitroguajacolicus, andPaenarthrobacter ureafaciens. Cells are rod shaped when growing exponentially and are coccoid when reaching the stationary growth phase. The species of this genus share high 16S rRNA similarities (>97.5%). The peptidoglycan type is A3α (Lys–Ala–Thr–Ala; A11.17). The quinone system is menaquinone MK‐9(H2). Minor amounts of MK‐9(H2) and MK‐10(H2) may be present as well. Major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, dimannosylglyceride, and monogalactosyldiacylglycerol. Major fatty acid is anteiso‐C15:0. High amounts of iso‐C15:0, iso‐C16:0, anteiso‐C17:0, and iso‐C14:0 may be present as well. The habitat is soil.DNA G + C content (mol%): 61.3–63.6.Type species:Paenarthrobacter aurescensBusse 2016, 25VP.
A
naerolinea
Authors: Yamada Takeshi , Sekiguchi Yuji 
Published: June 2018

An.ae.ro.li.ne'a. Gr. pref.
an
not; Gr. masc. n.
aer
air; L. fem. n.
linea
line; N.L. fem. n.
Anaerolinea
line‐shaped organisms not living in air.

Chloroflexi / Anaerolineae / Anaerolineales / Anaerolineaceae / Anaerolinea
Cells are nonmotile and Gram‐negative. Multicellular filaments are longer than 100 µm in length and 0.2–0.4 µm in width. Spore formation is not observed under any culture conditions. Thermophilic and neutrophilic. Strictly anaerobic chemoorganoheterotroph. No photosynthetic growth is observed. Yeast extract is required for growth. In the presence of yeast extract, carbohydrates and proteinaceous carbon sources are fermented as energy and carbon sources. Nitrate, fumarate, sulfate, sulfite, elemental sulfur, thiosulfate, and Fe (III)‐nitrilotriacetate (NTA) are not utilized as electron acceptors. Growth is stimulated in cocultivation with hydrogenotrophic methanogens. Known habitats include thermophilic anaerobic sludge.

DNA G + C content (mol%)
: 53.7–53.9 (genome sequence).

Type species
:
Anaerolinea thermophila
Sekiguchi, Yamada, Hanada, Ohashi, Harada and Kamagata 2003, 1850
VP
.
Pelolinea
Authors: Imachi Hiroyuki , Takaki Yoshihiro , Nakahara Nozomi 
Published: June 2018

Pe.lo.li'ne.a. Gr. adj.pelosdark colored, hence anaerobic mud; L. fem. n.linea, line; N.L. fem. n.Pelolinealine‐shaped organism living in anaerobic environments.Chloroflexi / Anaerolineae / Anaerolineales / Anaerolineaceae / PelolineaCells arefilamentous. Individual cells are longer than 10 µm. Cell width is 130–150 nm. Gram‐stain‐negative. Spore formation and motility are not observed.Strictly anaerobic and chemoorganotrophic metabolism with sugars and polysaccharides. Yeast extract is required for growth. Optimum growth temperature range is 25–30°C. Optimum pH for growth is 7.0. NaCl concentration range of growth is between 0 and 50 g/l with optimum growth in 15 g/l. The major fatty acids are monounsaturated C18:1ω9 and C16:1ω7 and saturated C18:0and C16:0. The dominant intact polar lipids are diacylglyceride and sphingolipid core lipid structures with monoglycosidic, mixed phosphomonoglycosidic, and fatty‐acid‐modified monoglycosidic polar head groups. Members of the classAnaerolineaewithin the phylumChloroflexi. Habitats are a wide variety of anaerobic environments.DNA G + C content (mol%): 52.4 (HPLC)/50.6 (complete genome sequence).Type species:Pelolinea submarinaImachi, Sakai, Lipp, Miyazaki, Saito, Yamanaka, Hinrichs, Inagaki, and Takai 2014, 817VP.
Efficient and Enantioselective Rhodium(I)‐Catalyzed Arylation of α‐Ketoesters: Synthesis of (S)‐Flutriafol
Authors: Chang Chiung‐An , Uang Tsung‐Ying , Jian Jia‐Hong , Zhou Meng‐Yi , Chen Ming‐Liang , Kuo Ting‐Shen , Wu Ping‐Yu , Wu Hsyueh‐Liang 
Published: June 2018

AbstractAn enantioselective addition of arylboronic acids and α‐ketoesters promoted by a Rhodium(I)‐chiral diene catalyst is reported. The transformation proceeds regioselectively in the presence of as low as 0.5 mol% of the catalyst generated in situ from a Rhodium(I) salt and diene L1, which is a bicyclo[2.2.1] ligand scaffold bearing 2,5 dinaphthyl substituents, to afford tertiary chiral α‐hydroxy esters with high stereoselectivities (90–99% ee's) and in 50–>99% yields. The method provides an expeditious and enantioselective synthesis of (S)‐flutriafol.magnified image
Enantioselective Construction of Vicinal Sulfur‐containing Tetrasubstituted Stereocenters via Organocatalyzed Mannich‐Type Addition of Rhodanines to Isatin Imines
Authors: Huang Qiuhong , Zhang Lili , Cheng Yuyu , Li Pengfei , Li Wenjun 
Published: June 2018

AbstractThe catalyzed enantioselective nucleophilic addition of rhodanines has been established by employing isatin‐derived imines as suitable partners under chiral squaramide catalysis. By using this strategy, the resulting 3‐substituted 3‐amino‐2‐oxindoles featuring both rhodanine and vicinal sulfur‐containing tetrasubstituted stereocenters structural motifs were obtained in high yields with excellent enantioselectivities and diastereoselectivities.magnified image
Palladium‐Catalyzed Solvent‐Dependent Divergent Synthesis of Benzylformamides
Authors: Wu Fu‐Peng , Peng Jin‐Bao , Qi Xinxin , Ying Jun , Wu Xiao‐Feng 
Published: June 2018

AbstractA palladium‐catalyzed carbonylative cascade procedure for the synthesis of benzylformamides from aryl halides has been developed. The properties of the solvent chosen play a decisive role on the selectivity of the outcomes. Non‐coordinative solvent facilitates the coordination and insertion of CO to the amino‐palladium intermediate which then provides the formamides products. While the use of coordinative solvent leads to the formation of amines. A series of functional groups, including ester, ketone, nitro and cyano, were well tolerated under our conditions. The corresponding benzylformamides products were prepared in moderate to good yields without further optimization.magnified image
Pd‐Catalyzed Decarboxylative Asymmetric Protonation of Sterically Hindered α‐Aryl Lactones and Dihydrocoumarins
Authors: James Jinju , Akula Ramulu , Guiry Patrick J. 
Published: June 2018

AbstractPd‐catalyzed decarboxylative asymmetric protonation (DAP) has been developed for sterically hindered α‐aryl lactone and dihydrocoumarin substrates. Optimization studies were conducted using δ‐lactone‐ and dihydrocoumarin‐derived α‐aryl, β‐oxo‐allyl esters with 2,4,6‐trimethoxyphenyl as the aryl substituent. In the absence of a chiral P,N‐ligand, (1R,2S)‐(−)ephedrine, a cheap and readily available chiral proton donor, induced enantioselectivities of up to 92% ee and 88% ee with lactone and dihydrocoumarin substrates, respectively. Bulky aryl groups containing di‐ortho substitutions and naphthyl groups gave the highest enantioselectivities of up to 92% and 86%, respectively. A stereochemical rationale is proposed to explain the preferred sense of asymmetric induction.magnified image
An industrial‐scale synthesis of biodegradable soluble in organic solvents butyric–acetic chitin copolyesters
Authors: Draczynski Zbigniew , Bogun Maciej , Sujka Witold , Kolesinska Beata 
Published: June 2018

AbstractThe article presents the results of study related to upscaling chitin esterification reaction to the industrial scale. The obtained results allowed for creation of a product which can later be used in manufacturing biologically active dressing materials. The implementation of the laboratory scale showed that there is a possibility of upscaling the procedure to make it suitable for the needs of industry. Problems and risks associated with the transition to an industrial scale, such as process temperature control, time of the reaction and the method of isolation of product (emptying the industrial reactor from the reaction mixture), have been presented and discussed. The results of the tests showed the interdependence between the stages of production process, both in laboratory and in industrial scale. The realization of the presented project enabled solving the problems arising both from the preparation to the synthesis, carrying out the process itself, and obtaining the final product. An important factor in this case was to obtain the same physicochemical properties of the final product on both laboratory and industrial scale. The tests of biodegradation of butyric–acetic chitin copolyester in fresh human plasma and blood serum confirmed that two enzymatic degradation processes occur due to the presence of the enzymes in these body fluids. At the same time, enzymatic hydrolysis of ester bonds and enzymatic hydrolysis of the glycoside bonds of the main polysaccharide chain take place.
3D printed self‐expandable vascular stents from biodegradable shape memory polymer
Authors: Jia Han , Gu Shu‐Ying , Chang Kun 
Published: June 2018

AbstractConsidering the high in‐stent restenosis and stent thrombosis rate of stent implantation with balloon angioplasty, self‐expandable biodegradable vascular stent was prepared by 3D printing from shape memory polylactide (PLA). The printed stent could be compressed into a temporary shape with thinner diameter easy for implantation. The compressed stent had excellent shape fixity and could maintain its temporary shape at room temperature for storage. However, it could self‐expand to its initial shape after being implanted due to the heat triggering of shape memory polylactide. The longitudinal contraction or radical expansion under its working condition was carried out by finite element analysis. The combination of self‐expansion of biodegradable shape memory PLA with personalized designing of 3D printing provides the vascular stent a future solution for cardiovascular disease treatment.
Elucidating Self‐Assembling Peptide Aggregation via Morphoscanner: A New Tool for Protein‐Peptide Structural Characterization
Authors: Saracino Gloria A. A. , Fontana Federico , Jekhmane Shehrazade , Silva João Medeiros , Weingarth Markus , Gelain Fabrizio 
Published: June 2018

AbstractSelf‐assembling and molecular folding are ubiquitous in Nature: they drive the organization of systems ranging from living creatures to DNA molecules. Elucidating the complex dynamics underlying these phenomena is of crucial importance. However, a tool for the analysis of the various phenomena involved in protein/peptide aggregation is still missing. Here, an innovative software is developed and validated for the identification and visualization of b‐structuring and b‐sheet formation in both simulated systems and crystal structures of proteins and peptides. The novel software suite, dubbed Morphoscanner, is designed to identify and intuitively represent b‐structuring and b‐sheet formation during molecular dynamics trajectories, paying attention to temporary strand‐strand alignment, suboligomer formation and evolution of local order. Self‐assembling peptides (SAPs) constitute a promising class of biomaterials and an interesting model to study the spontaneous assembly of molecular systems in vitro. With the help of coarse‐grained molecular dynamics the self‐assembling of diverse SAPs is simulated into molten aggregates. When applied to these systems, Morphoscanner highlights different b‐structuring schemes and kinetics related to SAP sequences. It is demonstrated that Morphoscanner is a novel versatile tool designed to probe the aggregation dynamics of self‐assembling systems, adaptable to the analysis of differently coarsened simulations of a variety of biomolecules.
Immunotherapy for pancreatic cancer: Barriers and breakthroughs
Authors: Torphy Robert J. , Zhu Yuwen , Schulick Richard D. 
Published: June 2018

AbstractImmunotherapy is a rapidly growing field and represents a paradigm shift in the treatment of malignancies as it offers a new therapeutic approach beyond surgery, conventional chemotherapy, and radiation treatment. Targeting immune checkpoints, such as cytotoxic T‐lymphocyte‐associated antigen 4 and programmed death 1/programmed death ligand 1 has had immense clinical success resulting in sustained treatment response for a subset of patients with certain malignancies such as melanoma, non‐small‐cell lung cancer, urothelial carcinoma, squamous cell carcinoma of the head and neck, renal cell cancer, hepatocellular cancer, and metastatic colorectal cancer. Importantly, there has been limited success in the use of immunotherapy in the treatment of pancreatic cancer. Investigation into the complex tumor microenvironment of pancreatic cancer that is composed of immune cells, stromal cells, and extracellular matrix proteins has begun to shed light on important attributes of this microenvironment that act as barriers to the effective use of immunotherapy. In this review, we will discuss the progress that has been made in treating pancreatic cancer with immunotherapy, the barriers that have limited treatment success, and breakthroughs with combination treatments that hold promise for the future.
Clinical characteristics and prognostic factors of sinonasal undifferentiated carcinoma: a multicenter study
Authors: de Bonnecaze Guillaume , Verillaud Benjamin , Chaltiel Leonor , Fierens Sylvestre , Chapelier Mark , Rumeau Cécile , Malard Olivier , Gavid Marie , Dufour Xavier , Righini Christian , Uro‐coste Emmanuelle , Rives Michel , Bach Christine , Baujat Bertrand , Janot François , de Gabory Ludovic , Vergez Sebastien 
Published: June 2018

BackgroundSinonasal undifferentiated carcinoma (SNUC) is a very rare entity with a poor prognosis. Due to the lack of studies on the subject, evidence is lacking concerning its management.MethodsA multicenter collaborative study was conducted to assess treatment strategy, oncological outcome, and prognostic factors.ResultsDefinitive analyses focused on 54 patients with a majority of advanced stage; the 3‐year overall survival (OS) and 3‐year recurrence‐free survival (RFS) rates were, respectively, 62.4% and 47.8%. During the follow‐up, 18 patients (33.3%) died, 10 (18.5%) developed metastases, 7 had lymph‐node involvement (13%), and 12 (22.2%) showed recurrence or local progression. In univariate analyses, treatment modalities associated with improved RFS were induction chemotherapy (p = 0.02) and intensity‐modulated radiotherapy (p = 0.007). In the multivariate analyses, only induction chemotherapy (p = 0.047, hazard ratio [HR] = 0.39) was significantly associated with improved RFS.ConclusionMultimodal therapies including induction chemotherapy and intensity‐modulated radiotherapy may improve the prognosis of SNUC; surgery might improve local control. Further multicenter studies are required.
Total Synthesis Establishes the Biosynthetic Pathway to the Naphterpin and Marinone Natural Products
Authors: Murray Lauren A. M. , McKinnie Shaun M. K. , Pepper Henry P. , Erni Reto , Miles Zachary D. , Cruickshank Michelle C. , López‐Pérez Borja , Moore Bradley S. , George Jonathan H. 
Published: June 2018

AbstractThe naphterpins and marinones are naphthoquinone meroterpenoids with an unusual aromatic oxidation pattern that is biosynthesized from 1,3,6,8‐tetrahydroxynaphthalene (THN). We propose that cryptic halogenation of THN derivatives by vanadium‐dependent chloroperoxidase (VCPO) enzymes is key to this biosynthetic pathway, despite the absence of chlorine in these natural products. This speculation inspired a total synthesis to mimic the naphterpin/marinone biosynthetic pathway. In validation of this biogenetic hypothesis, two VCPOs were discovered that interconvert several of the proposed biosynthetic intermediates.
Enhanced HPLC‐MS/MS method for the quantitative determination of the co‐administered drugs ceftriaxone sodium and lidocaine hydrochloride in human plasma following an intramuscular injection and application to a pharmacokinetic study
Authors: Mohamed Dalia , Kamal Maha 
Published: June 2018

AbstractA sensitive HPLC–MS/MS method was established for the quantification of ceftriaxone sodium (CFT) and lidocaine HCl (LDC) in human plasma utilizing cefixime (CFX) and tadalafil (TDA) as internal standards. The analytes were extracted from human plasma by protein precipitation using acetonitrile. Chromatographic separation was performed on Kinetex C18 (50.0 × 4.6 mm, 5 μm particle size) column with methanol–0.01 M ammonium acetate pH 6.4 (70: 30, v/v) as mobile phase. Multiple reaction monitoring involving the transitions 555.10 → 396.20, 235.20 → 86.00, 454.20 → 284.80 and 390.20 → 268.20 was utilized to quantify CFT, LDC, CFX and TDA, respectively, using a triple quadrupole mass spectrometer which was operated in positive ion mode. The method revealed linearity in the concentration range of 3.0–300.0 μg/mL for CFT and 3.0–300.0 ng/mL for LDC. The validation of the method was achieved in accordance to the US Food and Drug Administration guidelines. A pharmacokinetic study was performed on healthy Egyptian volunteers after intramuscular injection of sterile ceftriaxone sodium (1 g CFT dissolved in 3.5 mL of 1% LDC) after approval from the ethics committee. The pharmacokinetic parameters were: Cmax 141.15 ± 39.84 (μg/mL) and 55.02 ± 9.36 (ng/mL); tmax (h) 2.50 ± 0.50 and 1.5 ± 0.50; t½ (h) 7.30 ± 2.98 and 4.23 ± 1.96; and Kel (h−1) 0.10 ± 0.04 and 0.20 ± 0.13 for CFT and LDC, respectively.
The prestimulus default mode network state predicts cognitive task performance levels on a mental rotation task
Authors: Kamp Tabea , Sorger Bettina , Benjamins Caroline , Hausfeld Lars , Goebel Rainer 
Published: June 2018

AbstractBackgroundLinking individual task performance to preceding, regional brain activation is an ongoing goal of neuroscientific research. Recently, it could be shown that the activation and connectivity within large‐scale brain networks prior to task onset influence performance levels. More specifically, prestimulus default mode network (DMN) effects have been linked to performance levels in sensory near‐threshold tasks, as well as cognitive tasks. However, it still remains uncertain how the DMN state preceding cognitive tasks affects performance levels when the period between task trials is long and flexible, allowing participants to engage in different cognitive states.MethodsWe here investigated whether the prestimulus activation and within‐network connectivity of the DMN are predictive of the correctness and speed of task performance levels on a cognitive (match‐to‐sample) mental rotation task, employing a sparse event‐related functional magnetic resonance imaging (fMRI) design.ResultsWe found that prestimulus activation in the DMN predicted the speed of correct trials, with a higher amplitude preceding correct fast response trials compared to correct slow response trials. Moreover, we found higher connectivity within the DMN before incorrect trials compared to correct trials.ConclusionThese results indicate that pre‐existing activation and connectivity states within the DMN influence task performance on cognitive tasks, both effecting the correctness and speed of task execution. The findings support existing theories and empirical work on relating mind‐wandering and cognitive task performance to the DMN and expand these by establishing a relationship between the prestimulus DMN state and the speed of cognitive task performance.
Synthesis of 3‐Methylidene‐1‐tosyl‐2,3‐dihydroquinolin‐4(1H)‐ones as Potent Cytotoxic Agents
Authors: Koszuk Jacek , Bartosik Tomasz , Wojciechowski Jakub , Wolf Wojciech M. , Janecka Anna , Drogosz Joanna , Długosz Angelika , Krajewska Urszula , Mirowski Marek , Janecki Tomasz 
Published: June 2018

An efficient synthetic strategy to 3‐methylidene‐2,3‐dihydroquinolin‐4(1H)‐ones variously substituted in position 2 has been developed. The title compounds were synthesized in the reaction sequence involving reaction of diethyl methylphosphonate with methyl 2‐(tosylamino)benzoate, condensation of thus formed diethyl 2‐oxo‐2‐(2‐N‐tosylphenyl)ethylphosphonate with various aldehydes followed by successful application of the obtained 3‐(diethoxyphosphoryl)‐1,2‐dihydroquinolin‐4‐ols as Horner–Wadsworth–Emmons reagents for the olefination of formaldehyde. Also, enantioselective approach to the target compounds has been evaluated using 3‐dimenthoxyphosphoryl group as a chiral auxiliary. Single X‐ray crystal analysis of (2S)‐3‐(dimenthoxyphosphoryl)‐2‐phenyl‐1‐tosyldihydroquinolin‐4‐ol revealed the presence of strong resonance‐assisted hydrogen bond (RAHB). The obtained 3‐methylidene‐2,3‐dihydroquinolin‐4(1H)‐ones were then tested for their cytotoxic activity against two leukemia cell lines NALM‐6 and HL‐60 and a breast cancer MCF‐7 cell line. All compounds showed very high cytotoxic activity with the IC50 values mostly below 1 μm in all three cancer cell lines. The selected analogs were also tested on human umbilical vein endothelial cells (HUVEC) and on human mammary gland/breast cells (MCF‐10A) to evaluate their influence on normal cells. Since one of the most serious problems in cancer chemotherapy is the development of drug resistance, the mRNA levels and activity of ABCB1 transporter considered to be the most important factor engaged in drug resistance, were evaluated in MCF‐7 cells treated with two selected analogs. Both compounds were strong ABCB1 transporter inhibitors that could prevent efflux of anticancer drugs from cancer cells.
A Bioluminescent Ca2+ Indicator Based on a Topological Variant of GCaMP6s
Authors: Qian Yong , Rancic Vladimir , Wu Jiahui , Ballanyi Klaus , Campbell Robert E. 
Published: June 2018

AbstractFluorescent genetically encoded calcium ion indicators (GECIs) enable Ca2+ dynamics to be monitored in a diverse array of cell types and tissues. One drawback of green fluorescent GECIs, such as the widely used GCaMP6, is that the blue wavelengths of light used to excite the GECI also activate optogenetic actuators such as channelrhodopsins. Accordingly, it is particularly challenging simultaneously to use both optogenetic actuators and GECIs to both control and image cell signaling. Bioluminescence is an alternative imaging modality that circumvents this problem by avoiding the need for illumination for fluorescence excitation. Here, we report the development of a bioluminescent GECI, designated LUCI‐GECO1, based on efficient bioluminescent resonance energy transfer (BRET) between the NanoLuc luciferase and a topological variant of GCaMP6s. LUCI‐GECO1 is a sensitive ratiometric GECI that retains the highly optimized properties of GCaMP6s, as we demonstrate by imaging of chemically and optogenetically induced Ca2+ concentration changes in cultured cells and neurons.