bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of Laboratory-Developed Tests
Authors: Genzen Jonathan R 
Published: June 2019

Abstract

Objectives
To provide a clinical laboratory perspective on the Verifying Accurate Leading-edge IVCT Development Act (VALID) discussion draft. This potential legislative effort, if enacted, would overhaul the regulatory oversight of in vitro diagnostics (IVDs) in the United States and create a single system for regulation of conventional IVDs and laboratory-developed tests (LDTs).

Methods
A concise literature-based review of LDT regulation is presented followed by a discussion of key concerns pertinent to clinical laboratories that should be considered in future IVD regulatory reform efforts.

Results
Key issues identified include the importance of fostering innovation, preserving patient safety, protecting the practice of laboratory medicine, and minimizing undue regulatory burden. Clinical laboratories are not equivalent to manufacturing facilities and would therefore encounter challenges in implementing device-centric regulatory oversight models.

Conclusions
It is imperative that a clinical laboratory perspective on LDTs is understood and incorporated prior to advancement of future legislative proposals.
Does Provider Specialty Matter?
Authors: Stubblefield Jeannie M , Lasley Mary V , Virant Frank S , Dickerson Jane A 
Published: June 2019

Abstract

Objectives
Multiple practice guidelines discourage indiscriminate use of broad panels of allergen-specific IgE (sIgE) tests due to increased risk of false positives and misinterpretation of results. We provide an analytical framework to identify specialty-specific differences in ordering patterns and effectiveness, which can be used to improve test utilization.

Methods
Test results from a tertiary pediatric hospital were analyzed by ordering specialty to evaluate size of allergen workups. Positivity rates were analyzed to determine effectiveness in selecting tests with high positive pretest probabilities. Laboratory test menu components were also evaluated.

Results
Our findings demonstrate 29% of sIgE tests are ordered as part of broad workups (>20 sIgE tests/date of service) contrary to the recommended testing approach. Detailed descriptions of ordering patterns and positivity rates are provided.

Conclusions
This study provides a framework for using a cross-sectional analytical approach to assess test utilization patterns and evaluate components of laboratory testing menus.
Attractiveness is positively related to World Cup performance in male, but not female, biathletes
Authors: Fawcett Tim W , Ewans Jack , Lawrence Alice , Radford Andrew N 
Published: June 2019

AbstractWhole-organism performance capacity is thought to play a key role in sexual selection, through its impacts on both intrasexual competition and intersexual mate choice. Based on data from elite sports, several studies have reported a positive association between facial attractiveness and athletic performance in humans, leading to claims that facial correlates of sporting prowess in men reveal heritable or nonheritable mate quality. However, for most of the sports studied (soccer, ice hockey, American football, and cycling), it is not possible to separate individual performance from team performance. Here, using photographs of athletes who compete annually in a multi-event World Cup, we examine the relationship between facial attractiveness and individual career-best performance metrics in the biathlon, a multidisciplinary sport that combines target shooting and cross-country skiing. Unlike all previous studies, which considered only male athletes, we report relationships for both sportsmen and sportswomen. As predicted by evolutionary arguments, we found that male biathletes were judged more attractive if (unknown to the raters) they had achieved a higher peak performance (World Cup points score) in their career, whereas there was no significant relationship for female biathletes. Our findings show that elite male athletes display visible, attractive cues that reliably reflect their athletic performance.
The Whole Story: The Dilemma of the Domestic Violence Protection Order Narrative
Authors: Fitzgerald Robin , Douglas Heather 
Published: June 2019

AbstractThe complaint narrative, in which victims describe their experiences of abuse as part of the domestic violence protection order application process, has been questioned on the basis that quality and outcome might vary depending on who completes the form. Using a mixed-methods narrative analysis approach, we advance this inquiry by examining whether distinct ‘types’ of narratives can be identified across a sample of protection order application narratives from the state of Queensland in Australia. We find three distinct narrative types that are differently associated with who makes the application and the protection order outcome. The results underscore the unevenness in the protection order process and equitable access to justice more broadly.
The antero-dorsal precuneal cortex supports specific aspects of bodily awareness
Authors: Herbet Guillaume , Lemaitre Anne-Laure , Moritz-Gasser Sylvie , Cochereau Jérôme , Duffau Hugues 
Published: June 2019

Abstract
The precuneus is a functionally heterogeneous area located on the medial face of the parietal cortex, wedged between the occipital cortex and the paracentral lobule. In view of its topological positioning, this associative cortex is well-placed to play an important role in multisensory integration, specific aspects of which participate to bodily awareness. However, this potential implication remains unestablished. We assessed bodily awareness longitudinally in 14 rare patients who underwent a surgery for a low-grade glioma mainly infiltrating the precuneus. To determine the brain locus the most frequently affected in patients showing bodily awareness disorders, we first contrasted the resection cavity distributions of patients with versus without bodily awareness disorders. We next applied ‘lesion network mapping’ to identify the networks functionally coupled with lesion locations causing bodily awareness disorder. Bodily awareness disorders were observed in half of patients after surgery, especially alien hand, macrosomatognosia and fading limb. Importantly, a dissociation was revealed between the antero-dorsal precuneus (bodily awareness disorders) and postero-dorsal precuneus (no bodily awareness disorders). Furthermore, bodily awareness disorder-related regions were specifically connected to a network of sensorimotor regions while others were connected with the default network. Altogether, the present findings indicate a critical role of the antero-dorsal precuneus in specific aspects of bodily awareness and in the maintenance of body schema.
Biomarkers for malignant pleural mesothelioma: a meta-analysis
Authors: Gillezeau Christina N , van Gerwen Maaike , Ramos Julio , Liu Bian , Flores Raja , Taioli Emanuela 
Published: June 2019

Abstract
Malignant pleural mesothelioma (MPM) is a rare but aggressive cancer, and early detection is associated with better survival. Mesothelin, fibulin-3 and osteopontin have been suggested as screening biomarkers. The study conducted a meta-analysis of the mean differences of mesothelin, osteopontin and fibulin-3 in blood and pleural samples. PubMed searches were conducted for studies that measured levels of mesothelin, osteopontin and fibulin-3 in participants with MPM compared with malignancy, benign lung disease or healthy participants. Thirty-two studies with mesothelin levels, 12 studies with osteopontin levels and 9 studies with fibulin-3 levels were included in the meta-analysis. Statistically significant mean differences were seen between MPM patients and all other comparison groups for mesothelin blood and pleural levels. Statistically significant differences in blood osteopontin levels were seen between participants with benign lung disease and healthy participants compared with participants with MPM, but not when comparing participants with cancer with MPM participants. There were not enough studies that reported osteopontin levels in pleural fluid to complete a meta-analysis. Statistically significant differences were seen in both blood and pleural levels of fibulin-3 in MPM patients compared with all other groups. On the basis of these results, mesothelin and fibulin-3 levels appear to be significantly lower in all control groups compared with those with MPM, making them good candidates for screening biomarkers. Osteopontin may be a useful biomarker for screening healthy individuals or those with benign lung disease but would not be useful for screening patients with malignancies.
Oral Targeted Delivery by Nanoparticles Enhances Efficacy of an Hsp90 Inhibitor by Reducing Systemic Exposure in Murine Models of Colitis and Colitis-Associated Cancer
Authors: Yang Mei , Zhang Fang , Yang Chunhua , Wang Lixin , Sung Junsik , Garg Pallavi , Zhang Mingzhen , Merlin Didier 
Published: June 2019

AbstractBackground and AimsHeat shock protein 90 [Hsp90]-targeted therapy has been proposed as a promising strategy for the treatment of ulcerative colitis [UC] and colitis-associated cancer [CAC]. Systemic administration of the Hsp90 inhibitor, 17-AAG, was found to be profoundly protective in preclinical mouse models of inflammatory bowel disease [IBD]. However, the therapeutic potential of 17-AAG is limited by potential side effects associated with its systemic exposure and the modest bioavailability afforded by its oral administration.MethodsTo address these issues, we used a versatile single-step surface-functionalizing technique to prepare a 17-AAG oral delivery system using PLGA/PLA-PEG-FA nanoparticles [NP-PEG-FA/17-AAG].ResultsNP-PEG-FA could be efficiently taken up by mouse Colon-26 cells and activated Raw 264.7 cells in vitro and by inflamed mouse colitis tissues in vivo. The therapeutic efficacy of orally administrated NP-PEG-FA/17-AAG was evaluated in in vivo models using dextran sulphate sodium [DSS]-induced UC and azoxymethane [AOM]/DSS-induced CAC, and the results indicated that NP-PEG-FA/17-AAG significantly alleviated the symptoms of UC and CAC. More importantly, our inflamed colitis-targeted 17-AAG nano-formulation reduced systemic exposure and provided a degree of therapeutic response similar to that obtained by systemic administration [intraperitoneal] of 17-AAG, but at a ten-fold lower dose.ConclusionsWe describe a convenient, orally administrated 17-AAG delivery system that exhibits enhanced efficacy in UC and CAC therapy while reducing systemic exposure. This system may represent a promising therapeutic approach for treating UC and CAC.
The Effect of Photoperiods and Light Intensity on Mating Behavior and Reproduction of Grapholita molesta (Lepidoptera: Tortricidae)
Authors: Li Xianwei , Jia Xiaoting , Xiang Huiming , Diao Hongliang , Yan Yu , Wang Yi , Ma Ruiyan 
Published: June 2019

Abstract
Oriental fruit moth, Grapholita molesta (Busck), is a worldwide pest and is the dominant pest of rosaceous plants in northern China. Little is known, however, about the influence of abiotic factors on the daily life history events of this economically important pest, so here we evaluated the effect of different photoperiods (L:D) (0:24; 2:22; 4:20; 6:18; 8:16; 10:14; 12:12; 14:10; 15:9 [control]; 16:8; 18:6; 20:4; 22:2; and 24:0 h) and light intensities (50, 100, 200, 300, 400, and 500 lux) on the mating behavior and reproduction of oriental fruit moth. We found that oriental fruit moth adults with an 8, 9, or 10 h scotophase showed considerably more courtship behavior than those in other photoperiod treatments. Meanwhile, male fanning and male ‘crawling in a circle’ behaviors significantly decreased with increasing light intensity. Neither female or male oriental fruit moth longevity nor the egg hatch rate were influenced by photoperiod (P = 0.146; 0.271; 0.107) or light intensity (P = 0.268; 0.08; 0.123). In both photoperiod and light intensity treatments (P < 0.05), the number of eggs laid daily by females was significantly influenced by female age, decreasing with greater age. Female oriental fruit moth lifetime fecundity was influenced by photoperiod, with the 9, 10, 16, 18, and 22 h scotophase periods having higher fecundity than other treatments. Meanwhile, fecundity decreased with increasing light intensity.
Rhythm control for patients with atrial fibrillation complicated with heart failure in the contemporary era of catheter ablation: a stratified pooled analysis of randomized data
Authors: Chen Shaojie , Pürerfellner Helmut , Meyer Christian , Acou Willem-Jan , Schratter Alexandra , Ling Zhiyu , Liu Shaowen , Yin Yuehui , Martinek Martin , Kiuchi Marcio G , Schmidt Boris , Chun K R Julian 
Published: June 2019

Abstract

Aims
The optimal treatment for patients with atrial fibrillation (AF) and heart failure (HF) has been a subject of debate for years. We aimed to evaluate the efficacy and safety of rhythm control strategy in patients with AF complicated with HF regarding hard clinical endpoints.

Methods and results
Up-to-date randomized data comparing rhythm control using antiarrhythmic drugs (AADs) vs. rate control (Subset A) or rhythm control using catheter ablation vs. medical therapy (Subset B) in AF and HF patients were pooled. The primary outcomes were all-cause mortality, re-hospitalization, stroke, and thromboembolic events. A total of 11 studies involving 3598 patients were enrolled (Subset A: 2486; Subset B: 1112). As compared with medical rate control, the AADs rhythm control was associated with similar all-cause mortality [odds ratio (OR): 0.96, P = 0.65], significantly higher rate of re-hospitalization (OR: 1.25, P = 0.01), and similar rate of stroke and thromboembolic events (OR: 0.91, P = 0.76,); however, as compared with medical therapy, catheter ablation rhythm control was associated with significantly lower all-cause mortality (OR: 0.51, P = 0.0003), reduced re-hospitalization rate (OR: 0.44, P = 0.003), similar rate of stroke events (OR: 0.59, P = 0.27), greater improvement in left ventricular ejection fraction [weighted mean difference (WMD): 6.8%, P = 0.0004], lower arrhythmia recurrence (29.6% vs. 80.1%, OR: 0.04, P &lt; 0.00001), and greater improvement in quality of life (Minnesota Living with Heart Failure Questionnaire score) (WMD: −9.1, P = 0.007).

Conclusion
Catheter ablation as rhythm control strategy substantially improves survival rate, reduces re-hospitalization, increases the maintenance rate of sinus rhythm, contributes to preserve cardiac function, and improves quality of life for AF patients complicated with HF.
General practitioners’ involvement during survivorship care of colon cancer in the Netherlands: primary health care utilization during survivorship care of colon cancer, a prospective multicentre cohort study
Authors: Duineveld Laura A M , Molthof Hanneke , Wieldraaijer Thijs , van de Ven Anthony W H , Busschers Wim B , van Weert Henk C P M , Wind Jan 
Published: June 2019

Abstract

Background
Primary health care use increases when cancer is diagnosed. This increase continues after cancer treatment. More generalist care is suggested to improve survivorship care. It is unknown to what extent cancer-related symptoms are currently presented in primary care in this survivorship phase.

Objective
To analyse primary health care utilization of colon cancer patients during and after treatment with curative intent.

Methods
In a prospective multicentre cohort study among patients with curatively treated colon cancer, we describe the primary health care utilization during the first 5 years of follow-up. Data were collected at general practitioner (GP) practices during 6 months.

Results
Of 183 included participants, 153 (84%) consulted their GP resulting in 606 contacts (mean 3.3, standard deviation 3.01) with on average 0.9 contact for colon-cancer-related (CCR) problems in the 6-month study period. Median time after surgery at inclusion was 7.6 months (range 0–58). Abdominal pain and chemotherapy-related problems were the most frequently reported CCR reasons. Of the CCR contacts, 83% was managed in primary care. As time after surgery passed, the number of CCR contacts declined in patients without chemotherapy and remained constant in patients who received chemotherapy.

Conclusion
Colon cancer survivors contact their GP frequently also for reasons related to cancer. Currently, a formal role for GPs in survivorship care is lacking, but nevertheless GPs provide a substantial amount of care. Working agreements between primary and secondary care are necessary to formalize the GP’s role in order to improve the quality of survivorship care.
Robust growth of archaeal cells lacking a canonical single-stranded DNA-binding protein
Authors: Suzuki Shoji , Kurosawa Norio 
Published: June 2019

Abstract
Canonical single-stranded DNA-binding proteins (SSBs) are universally conserved helix-destabilizing proteins that play critical roles in DNA replication, recombination and repair. Many biochemical and genetic studies have demonstrated the importance of functional SSBs for all life forms. Herein, we report successful deletion of the gene encoding the only canonical SSB of the thermophilic crenarchaeon Sulfolobus acidocaldarius. Genomic sequencing of the ssb-deficient strain using illumina sequencing revealed that the canonical ssb gene is completely deleted from the genome of S. acidocaldarius. Phenotypic characterization demonstrated robust growth of the thermophilic archaeal cells lacking a canonical SSB, thereby demonstrating tolerance to the loss of a universal protein that is generally considered to be essential. Therefore, our work provides evidence that canonical SSBs are not essential for all life forms. Furthermore, on the basis of universal distribution and essentiality pattern of canonical SSBs, our findings can provide a conceptual understanding of the characteristics of early life forms before the last universal common ancestor.
Effects of intensified silviculture on timber production and its economic profitability in boreal Norway spruce and Scots pine stands under changing climatic conditions
Authors: Routa J , Kilpeläinen A , Ikonen V -P , Asikainen A , Venäläinen A , Peltola H 
Published: June 2019

Abstract
The aim of this study was to examine how intensified silviculture affects timber production (sawlogs and pulpwood) and its economic profitability (net present value [NPV], with 2 per cent interest rate) based on forest ecosystem model simulations. The study was conducted on Norway spruce and Scots pine stands located on medium-fertile upland forest sites under middle boreal conditions in Finland, under current climate and minor climate change (the RCP2.6 forcing scenario). In intensified silviculture, improved regeneration materials were used, with 10–20 per cent higher growth than the unimproved materials, and/or nitrogen (N) fertilization of 150 kg ha−1, once or twice during a rotation of 50–70 years. Compared to the baseline management regime, the use of improved seedlings, alone or together with N fertilization, increased timber production by up to 26–28 per cent and the NPV by up to 32–60 per cent over rotation lengths of 60–70 years, regardless of tree species (although more in spruce) or climate applied. The use of improved seedlings affected timber yield and NPV more than N fertilization. Minor climate change also increased these outcomes in Scots pine, but not in Norway spruce.
Examining Cumulative Inequality in the Association Between Childhood Socioeconomic Status and Body Mass Index From Midlife to Old Age
Authors: Lee Chioun , Park Soojin 
Published: June 2019

Abstract

Objectives
Socioeconomic status (SES) is among the strongest determinants of body mass index (BMI), particularly for women. For older populations, selection bias due to attrition is a large barrier to assessing the accumulation of inequality. Under multiple missing data mechanisms, we investigated the extent to which childhood and midlife SES affects BMI from midlife to old age and gender differences in the association.

Method
Data come from a longitudinal national study of 2,345 U.S. adults aged 40–54 at baseline. We used latent growth models to estimate BMI trajectory over a period of 20 years. We examined results under different missing data patterns and applied methods that account for nonrandom-selection bias.

Results
Compared with individuals who had higher childhood SES, individuals who had lower childhood SES have higher BMI in midlife and experience a faster increase in BMI between midlife and old age. The observed associations remain significant even after controlling for midlife SES. After addressing nonrandom selection, the gap in BMI between high and low childhood SES widens from midlife to old age for women.

Discussion
The findings provide new evidence of cumulative inequality among older adults, documenting increasing BMI inequality from midlife to old age, particularly for women from low-SES families.
A validated collection of mouse monoclonal antibodies to human glycosyltransferases functioning in mucin-type O-glycosylation
Authors: Steentoft Catharina , Yang Zhang , Wang Shengjun , Ju Tongzhong , Vester-Christensen Malene B , Festari María F , King Sarah L , Moremen Kelley , Larsen Ida S B , Goth Christoffer K , Schjoldager Katrine T , Hansen Lars , Bennett Eric P , Mandel Ulla , Narimatsu Yoshiki 
Published: June 2019

AbstractComplex carbohydrates serve a wide range of biological functions in cells and tissues, and their biosynthesis involves more than 200 distinct glycosyltransferases (GTfs) in human cells. The kinetic properties, cellular expression patterns and subcellular topology of the GTfs direct the glycosylation capacity of a cell. Most GTfs are ER or Golgi resident enzymes, and their specific subcellular localization is believed to be distributed in the secretory pathway according to their sequential role in the glycosylation process, although detailed knowledge for individual enzymes is still highly fragmented. Progress in quantitative transcriptome and proteome analyses has greatly advanced our understanding of the cellular expression of this class of enzymes, but availability of appropriate antibodies for in situ monitoring of expression and subcellular topology have generally been limited. We have previously used catalytically active GTfs produced as recombinant truncated secreted proteins in insect cells for generation of mouse monoclonal antibodies (mAbs) to human enzymes primarily involved in mucin-type O-glycosylation. These mAbs can be used to probe subcellular topology of active GTfs in cells and tissues as well as their presence in body fluids. Here, we present several new mAbs to human GTfs and provide a summary of our entire collection of mAbs, available to the community. Moreover, we present validation of specificity for many of our mAbs using human cell lines with CRISPR/Cas9 or zinc finger nuclease (ZFN) knockout and knockin of relevant GTfs.