bluuebunny/crossref_metadata_2025_split huggingface dataset:
Creatine supplementation impairs airway inflammation in an experimental model of asthma involving P2 × 7 receptor
Authors: Garcia Monique , Santos‐Dias Alana , Bachi André Luis Lacerda , Oliveira‐Junior Manoel Carneiro , Andrade‐Souza Adilson Santos , Ferreira Sérgio César , Aquino‐Junior Jefferson Comin Jonco , Almeida Francine Maria , Rigonato‐Oliveira Nicole Cristine , Oliveira Ana Paula Ligeiro , Savio Luiz Eduardo Baggio , Coutinho‐Silva Robson , Müller Tobias , Idzko Marco , Siepmann Timo , Vieira Rodolfo Paula 
Published: March 2019

AbstractCreatine (Cr) is a substrate for adenosine triphosphate synthesis, and it is the most used dietary supplement among professional and recreative athletes and sportsmen. Creatine supplementation may increase allergic airway response, but the cellular and molecular mechanisms are unknown. We used murine model of OVA‐induced chronic asthma and showed that Cr supplementation increased total proteins, ATP level, lymphocytes, macrophages, and IL‐5 levels in BALF, as well as IL‐5 in the supernatant of re‐stimulated mediastinal lymph nodes. IL‐5 and IL‐13 expression by epithelial cells and by peribronchial leukocytes were increased by Cr. Cr augmented the expression of P2 × 7 receptor by peribronchial leukocytes and by epithelial cells, and increased the accumulation of eosinophils in peribronchial space and of collagen fibers in airway wall. In human cells, while Cr induced a release of ATP, IL‐6, and IL‐8 from BEAS‐2B cells, whole blood cells, such as eosinophils, and CD4+ T cells, P2 × 7 receptor inhibitor (A740003) reduced such effects, as denoted by reduced levels of ATP, IL‐6, and IL‐8. Therefore, Cr supplementation worsened asthma pathology due to activation of airway epithelial cells and peribronchial leukocytes, involving purinergic signaling.
The B cell novel protein 1 (BCNP1) regulates BCR signaling and B cell apoptosis
Authors: Hong Rongjian , Lai Nannan , Ouchida Rika , Xiong Ermeng , Zhou Yang , Min Qing , Liu Jun , Tang Yue , Hikida Masaki , Tsubata Takeshi , Wang Yanqing , Wang Ji‐Yang 
Published: March 2019

AbstractThe BCR plays a central role in B cell development, survival, activation, and differentiation. We have identified the B cell novel protein 1 (BCNP1) as a new regulator of BCR signaling. BCNP1 contains a pleckstrin homology domain, three proline‐rich motifs, and a potential SH2 binding site, and is predominantly expressed by B cells. We found that BCNP1 overexpression in WEHI231 immature B cells potentiated α‐IgM‐induced apoptosis. Conversely, BCNP1‐deficient WEHI231 cells, generated by CRISPR‐Cas9‐mediated genome editing, exhibited reduced apoptosis after BCR crosslinking. Biochemical analyses revealed that BCNP1 physically interacted with the B cell linker protein (BLNK), Grb2, and PLCγ2. Moreover, absence of BCNP1 resulted in accelerated dephosphorylation of BLNK, reduced phosphorylation of SYK and PLCγ2, and decreased Ca2+ influx after BCR crosslinking. These results demonstrate that BCNP1 promotes BCR signaling by modulating the phosphorylation of BLNK, SYK, and PLCγ2.
Peripheral and systemic antigens elicit an expandable pool of resident memory CD8+ T cells in the bone marrow
Authors: Pascutti Maria Fernanda , Geerman Sulima , Collins Nicholas , Brasser Giso , Nota Benjamin , Stark Regina , Behr Felix , Oja Anna , Slot Edith , Panagioti Eleni , Prier Julia E. , Hickson Sarah , Wolkers Monika C. , Heemskerk Mirjam H.M. , Hombrink Pleun , Arens Ramon , Mackay Laura K. , van Gisbergen Klaas P.J.M. , Nolte Martijn A. 
Published: March 2019

AbstractBM has been put forward as a major reservoir for memory CD8+ T cells. In order to fulfill that function, BM should “store” memory CD8+ T cells, which in biological terms would require these “stored” memory cells to be in disequilibrium with the circulatory pool. This issue is a matter of ongoing debate. Here, we unequivocally demonstrate that murine and human BM harbors a population of tissue‐resident memory CD8+ T (TRM) cells. These cells develop against various pathogens, independently of BM infection or local antigen recognition. BM CD8+ TRM cells share a transcriptional program with resident lymphoid cells in other tissues; they are polyfunctional cytokine producers and dependent on IL‐15, Blimp‐1, and Hobit. CD8+ TRM cells reside in the BM parenchyma, but are in close contact with the circulation. Moreover, this pool of resident T cells is not size‐restricted and expands upon peripheral antigenic re‐challenge. This works extends the role of the BM in the maintenance of CD8+ T cell memory to include the preservation of an expandable reservoir of functional, non‐recirculating memory CD8+ T cells, which develop in response to a large variety of peripheral antigens.
Determination of working resistance based on movement type of the first subordinate key stratum in a fully mechanized face with large mining height
Authors: Li Bo , Liang Yunpei , Zou Quanle 
Published: March 2019

AbstractThe increase in extraction height will increase the mining‐induced overlying strata failure height. In this scenario, the strata pressure behavior is strong in a fully mechanized face with large mining height (FMFLMH), which frequently causes coal wall falls, roof falls, and hydraulic support failure accidents (e.g., support closure and hydraulic column damage). The key to solving these issues is to determine support's working resistance of the FMFLMH. In this paper, comprehensive theoretical analysis, numerical simulation, and field observation were applied to determine the support's working resistance in the FMFLMH based on movement type of the first subordinate key stratum (SKS 1). First, six kinds of movement types of SKS 1 in the FMFLMH are found and defined by theoretical analysis and numerical simulation, which are the direct caving movement type of cantilever structure (direct caving), the double‐sided rotation movement type of cantilever structure (double‐side rotation), the quadratic rotation movement type of cantilever structure (quadratic rotation), the alternative movement type of cantilever structure hinged structure (alternate hinged), the voussoir beam structure movement type (voussoir), and the short voussoir beam structure movement type (short voussoir), respectively. Besides, based on this, the support load calculation model of each movement type was established, and a formula for the support working resistance of each movement type was obtained. Finally, the correctness of the formulae for the support working resistance under six types of movement of SKS 1 were verified using measurement data from four FMFLMHs in China. These research results have important guiding significance for reasonable selection of support and ensuring safe mining of the FMFLMH.
From Grain to Floodplain: Evaluating heterogeneity of floodplain hydrostatigraphy using sedimentology, geophysics, and remote sensing
Authors: Malenda H.F. , Sutfin N.A. , Guryan G. , Stauffer S. , Rowland J.C. , Williams K.H. , Singha K. 
Published: March 2019

AbstractStratigraphy is a fundamental component of floodplain heterogeneity and hydraulic conductivity and connectivity of alluvial aquifers, which affect hydrologic processes such as groundwater flow and hyporheic exchange. Watershed‐scale hydrological models commonly simplify the sedimentology and stratigraphy of floodplains, neglecting natural floodplain heterogeneity and anisotropy. This study, conducted in the upper reach of the East River in the East River Basin, Colorado, USA, combines point‐, meander‐, and floodplain‐scale data to determine key features of alluvial aquifers important for estimating hydrologic processes. We compare stratigraphy of two meanders with disparate geometries to explore floodplain heterogeneity and connectivity controls on flow and transport. Meander shape, orientation, and internal stratigraphy affected residence time estimates of laterally exchanged hyporheic water. Although the two meanders share a sediment source, vegetation, and climate, their divergent river migration histories resulted in contrasting meander hydrofacies. In turn, the extent and orientation of these elements controlled the effective hydraulic conductivity and, ultimately, estimates of groundwater transport and hyporheic residence times. Additionally, the meanders’ orientation relative to the valley gradient impacted the hydraulic gradient across the meanders—a key control of groundwater velocity. Lastly, we combine our field data with remotely sensed data and introduce a potential approach to estimate key hydrostratigraphic packages across floodplains. Prospective applications include contaminant transport studies, hyporheic models, and watershed models. © 2019 John Wiley & Sons, Ltd.
Integrating bioenergy and food production on degraded landscapes in Indonesia for improved socioeconomic and environmental outcomes
Authors: Rahman Syed Ajijur , Baral Himlal , Sharma Roshan , Samsudin Yusuf B. , Meyer Maximilian , Lo Michaela , Artati Yustina , Simamora Trifosa Iin , Andini Sarah , Leksono Budi , Roshetko James M. , Lee Soo Min , Sunderland Terry 
Published: March 2019

AbstractGrowing bioenergy crops on degraded and underutilized land is a promising solution to meet the requirement for energy security, food security, and land restoration. This paper assesses the socioeconomic and environmental benefits of agroforestry systems based on nyamplung (tamanu) (Calophyllum inophyllum L.) in the Wonogiri district of Central Java, Indonesia. Data were collected through field observations and focus group discussions involving 20 farmers who intercrop nyamplung with maize, rice, and peanuts and utilize the species in honey production. Calculating each crop's net present value (NPV) demonstrates that when grown as monocultures, staple crops rice and peanuts lead to negative profitability, while maize generates only a marginal profit; yet honey production utilizing nyamplung produces a NPV nearly 300 times greater than maize. However, when utilizing nyamplung, honey is also the commodity most sensitive to decreases in production, followed by nyamplung–peanut and nyamplung–rice combinations. While decreases in production have little effect on the NPVs of rice, peanuts, and maize, these annual crops can only be cultivated for a maximum of 6 years within the nyamplung's 35‐year cycle, due to canopy closure after this time. Nyamplung‐based agroforestry systems can provide economic, social, and environmental gains on different scales. However, when considering the high profit potential of nyamplung combined with honey production, further research is needed to improve and develop bee husbandry practices so this becomes a viable option for local farmers.
Why do EMD‐based methods improve prediction? A multiscale complexity perspective
Authors: Dong Jichang , Dai Wei , Tang Ling , Yu Lean 
Published: March 2019

AbstractEmpirical mode decomposition (EMD)‐based ensemble methods have become increasingly popular in the research field of forecasting, substantially enhancing prediction accuracy. The key factor in this type of method is the multiscale decomposition that immensely mitigates modeling complexity. Accordingly, this study probes this factor and makes further innovations from a new perspective of multiscale complexity. In particular, this study quantitatively investigates the relationship between the decomposition performance and prediction accuracy, thereby developing (1) a novel multiscale complexity measurement (for evaluating multiscale decomposition), (2) a novel optimized EMD (OEMD) (considering multiscale complexity), and (3) a novel OEMD‐based forecasting methodology (using the proposed OEMD in multiscale analysis). With crude oil and natural gas prices as samples, the empirical study statistically indicates that the forecasting capability of EMD‐based methods is highly reliant on the decomposition performance; accordingly, the proposed OEMD‐based methods considering multiscale complexity significantly outperform the benchmarks based on typical EMDs in prediction accuracy.
Constructing and evaluating core inflation measures from component‐level inflation data
Authors: Gamber Edward N. , Smith Julie K. 
Published: March 2019

AbstractThis paper undertakes a comprehensive examination of 10 measures of core inflation and evaluates which measure produces the best forecast of headline inflation out‐of‐sample. We use the Personal Consumption Expenditure Price Index as our measure of inflation. We use two sets of components (17 and 50) of the Personal Consumption Expenditure Price Index to construct these core inflation measures and evaluate these measures at the three time horizons (6, 12 and 24 months) most relevant for monetary policy decisions. The best measure of core inflation for both sets of components and over all time horizons uses weights based on the first principal component of the disaggregated (component‐level) prices. Interestingly, the results vary by the number of components used; when more components are used the weights based on the persistence of each component is statistically equivalent to the weights generated by the first principal component. However, those forecasts using the persistence of 50 components are statistically worse than those generated using the first principal component of 17 components. The statistical superiority of the principal component method is due to the fact that it extracts (in the first principal component) the common source of variation in the component level prices that accurately describes trend inflation over the next 6–24 months.
Chronic activation of the relaxin‐3 receptor on GABA neurons in rat ventral hippocampus promotes anxiety and social avoidance
Authors: Rytova Valeria , Ganella Despina E. , Hawkes David , Bathgate Ross A. D. , Ma Sherie , Gundlach Andrew L. 
Published: March 2019

AbstractAnxiety disorders are highly prevalent in modern society and better treatments are required. Key brain areas and signaling systems underlying anxiety include prefrontal cortex, hippocampus, and amygdala, and monoaminergic and peptidergic systems, respectively. Hindbrain GABAergic projection neurons that express the peptide, relaxin‐3, broadly innervate the forebrain, particularly the septum and hippocampus, and relaxin‐3 acts via a Gi/o‐protein‐coupled receptor known as the relaxin‐family peptide 3 receptor (RXFP3). Thus, relaxin‐3/RXFP3 signaling is implicated in modulation of arousal, motivation, mood, memory, and anxiety. Ventral hippocampus (vHip) is central to affective and cognitive processing and displays a high density of relaxin‐3‐positive nerve fibers and RXFP3 binding sites, but the identity of target neurons and associated effects on behavior are unknown. Therefore, in adult, male rats, we assessed the neurochemical nature of hippocampal RXFP3 mRNA‐expressing neurons and anxiety‐like and social behavior following chronic RXFP3 activation in vHip by viral vector expression of an RXFP3‐selective agonist peptide, R3/I5. RXFP3 mRNA detected by fluorescent in situ hybridization was topographically distributed across the hippocampus in somatostatin‐ and parvalbumin‐mRNA expressing GABA neurons. Chronic RXFP3 activation in vHip increased anxiety‐like behavior in the light–dark box and elevated‐plus maze, but not the large open‐field test, and reduced social interaction with a conspecific stranger. Our data reveal disruptive effects of persistent RXFP3 signaling on hippocampal GABA networks important in anxiety; and identify a potential therapeutic target for anxiety disorders that warrants further investigation in relevant preclinical models.
Numerical validation of natural convection heat transfer with horizontal rectangular fin array using straight knurling patterns on fins—correlation for Nusselt number
Authors: Chikurde Rahul C. , Kothavale Basavraj S. , Sane Narayan. K. 
Published: March 2019

AbstractNatural convection heat transfer from horizontal rectangular fin array with various knurling patterns has been studied experimentally by the present authors to investigate the effect of knurl‐produced surface roughness of fin on the heat transfer rate. The parameters like knurling height from base, knurling depth, fin spacing, and supply wattage were considered for parametric study. In the present paper, numerical method (CFD) is used to simulate natural convection phenomena with knurled fins and results are validated with the experimental data available from literature. The numerical results show similar trends compared with experimental data and one can use this method to study various fin configurations for knurling patterns. The flow patterns from experiments and numerical method are compared for different supply wattages and fin spacing to back up the conclusion. It is also observed that the variation in nondimensional roughness depth and spacing (D
kn/H and S/H) have more effect on Nusselt number than roughness height parameter (H
kn/H). Further, the method is extended to study numerically large number of fin configurations with knurling patterns to gather sufficient data for Nusselt number with respect to fin geometric parameters as above and establish correlation for heat transfer coefficient for such type of fins.
Design considerations and their effects on the operation and maintenance cost in solar‐powered desalination plants
Authors: Kabeel A. E. , El‐Said Emad M. S. , Dafea Salah A. 
Published: March 2019

AbstractThe most critical aspects of any desalination plant are maintenance and operation costs. For solar water desalination, cost reduction and production of superior water quality are still crucial factors that attract researchers. Faults in the design of desalination systems can lead to many maintenance and operational problems in these systems and may lead to increasing water costs and/or decreasing quality of water. This paper offers an analytical study of design considerations and its effects on the operation and maintenance of solar‐powered desalination plants such as plant capacity, location, technology, demands, product water specifications, lifetime, availability, reliability, and input resources. This paper also provides an overview of the factors and components of desalination costs compared with other water supply alternatives and traditional desalination methods; discusses challenges and perceptions; and highlights recent developments in the solar water desalination technology that affect the total cost of water delivery and relationships with operation and maintenance.
Surface‐enhanced Raman scattering‐active photonic crystal fiber probe: Towards next generation liquid biopsy sensor with ultra high sensitivity
Authors: Dinish U. S , Beffara Flavien , Humbert Georges , Auguste Jean‐Louis , Olivo Malini 
Published: March 2019

AbstractThe tremendous enhancement factors that surface‐enhanced Raman scattering (SERS) possesses coupled with the flexibility of photonic crystal fibers (PCFs) pave the way to a new generation of ultrasensitive biosensors. Thanks to the unique structure of PCFs, which allows direct incorporation of an analyte into the axially aligned air channels, interaction between the analyte and excitation light could be increased many folds leading to flexible, reliable and sensitive probes that can be used in preclinical or clinical biosensing. SERS‐active PCF probes provide unique opportunity to develop an opto‐fluidic liquid biopsy needle sensor that enables one‐step integrated sample collection and testing for disease diagnosis. Specificity being a key parameter to biosensors, the PCF inside the biopsy needle could be functionalized with targeting moieties to detect specific biomarkers. In this review article, we present some of the most promising recent biosensors based on PCFs including hollow‐core PCFs, suspended‐core PCFs and side‐channel PCFs. We provide a wide range of applications of such platform using Raman spectroscopy, label free SERS or labeled SERS detection and analyze some of the main challenges to be addressed for translating it to a clinically viable next generation sensitive biopsy needle sensing probe.
Assessing the acute effects of prenatal synthetic cannabinoid exposure on murine fetal brain vasculature using optical coherence tomography
Authors: Raghunathan Raksha , Liu Chih‐Hao , Kouka Amur , Singh Manmohan , Miranda Rajesh C. , Larin Kirill V. 
Published: March 2019

AbstractMarijuana is one of the most commonly abused substances during pregnancy. Synthetic cannabinoids (SCBs) are a group of heterogeneous compounds that are 40‐ to 600‐fold more potent than Δ9‐tetrahydrocannabinol, the major psychoactive component of marijuana. With SCBs being legally available for purchase and the prevalence of unplanned pregnancies, the possibility of prenatal exposure to SCBs is high. However, the effects of prenatal SCB exposure on embryonic brain development are not well understood. In this study, we use complex correlation mapping optical coherence angiography to evaluate changes in murine fetal brain vasculature in utero, minutes after maternal exposure to an SCB, CP‐55940. Results showed a significant decrease (P < 0.05) in fetal brain vessel diameter, length fraction and area density when compared to the sham group. This preliminary study shows that acute prenatal exposure to an SCB resulted in significant fetal brain vasoconstriction during the peak period for brain development.
Corneal relaxation time estimation as a function of tear oxygen tension in human cornea during contact lens wear
Authors: Del Castillo Luis Felipe , Ramírez‐Calderón Juanibeth G. , Del Castillo Roxana M. , Aguilella‐Arzo Marcel , Compañ Vicente 
Published: March 2019

AbstractThe purpose is to estimate the oxygen diffusion coefficient and the relaxation time of the cornea with respect to the oxygen tension at the cornea–tears interface. Both findings are discussed. From the experimental data provided by Bonanno et al., the oxygen tension measurements in vivo for human cornea–tears–contact lens (CL), the relaxation time of the cornea, and their oxygen diffusion coefficient were obtained by numerical calculation using the Monod‐kinetic model. Our results, considering the relaxation time of the cornea, observe a different behavior. At the time less than 8 s, the oxygen diffusivity process is upper‐diffusive, and for the relaxation time greater than 8 s, the oxygen diffusivity process is lower‐diffusive. Both cases depend on the partial pressure of oxygen at the entrance of the cornea. The oxygen tension distribution in the cornea–tears interface is separated into two different zones: one for conventional hydrogels, which is located between 6 and 75 mmHg, with a relaxation time included between 8 and 19 s, and the other zone for silicone hydrogel CLs, which is located at high oxygen tension, between 95 and 140 mmHg, with a relaxation time in the interval of 1.5–8 s. It is found that in each zone, the diffusion coefficient varies linearly with the oxygen concentration, presenting a discontinuity in the transition of 8 s. This could be interpreted as an aerobic‐to‐anaerobic transition. We attribute this behavior to the coupling formalism between oxygen diffusion and biochemical reactions to produce adenosine triphosphate. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:14–21, 2020.