bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Drosophila Afadin and ZO-1 homologs Canoe and Polychaetoid act in parallel to maintain epithelial integrity when challenged by adherens junction remodeling
Authors: Manning Lathiena A. , Perez-Vale Kia Z. , Schaefer Kristina N. , Sewell Mycah T. , Peifer Mark 
Published: April 2019

AbstractDuring morphogenesis cells must change shape and move without disrupting tissue integrity. This requires cell-cell junctions to allow dynamic remodeling while resisting force generated by the actomyosin cytoskeleton. Multiple proteins play roles in junctional-cytoskeletal linkage, but the mechanisms by which they act remain unclear. Drosophila Canoe maintains adherens junction-cytoskeletal linkage during gastrulation. Canoe’s mammalian homolog Afadin plays similar roles in cultured cells, working in parallel with ZO-1 proteins, particularly at multicellular junctions. We took these insights back into the fly embryo, exploring how cells maintain epithelial integrity when challenged by adherens junction remodeling during germband extension and dorsal closure. We found Canoe helps cells maintain junctional-cytoskeletal linkage when challenged by the junctional remodeling inherent in mitosis, cell intercalation and neuroblast invagination, or by forces generated by the actomyosin cable at the leading edge. However, even in the absence of Canoe many cells retain epithelial integrity. This is explained by a parallel role played by the ZO-1 homolog Polychaetoid. In embryos lacking both Canoe and Polychaetoid, cell junctions fail early, with multicellular junctions especially sensitive, leading to widespread loss of epithelial integrity. Our data suggest Canoe and Polychaetoid stabilize Bazooka/Par3 at cell-cell junctions, helping maintain balanced apical contractility and tissue integrity.
Integrating Protein Localization with Automated Signaling Pathway Reconstruction
Authors: Youssef Ibrahim , Law Jeffrey , Ritz Anna 
Published: April 2019

AbstractUnderstanding cellular responses via signal transduction is a core focus in systems biology. Tools to automatically reconstruct signaling pathways from protein-protein interactions (PPIs) can help biologists generate testable hypotheses about signaling. However, automatic reconstruction of signaling pathways suffers from many interactions with the same confidence score leading to many equally good candidates. Further, some reconstructions are biologically misleading due to ignoring protein localization information. We proposeLocPL, a method to improve the automatic reconstruction of signaling pathways from PPIs by incorporating information about protein localization in the reconstructions. The method relies on a dynamic program to ensure that the proteins in a reconstruction are localized in cellular compartments that are consistent with signal transduction from the membrane to the nucleus.LocPLand existing reconstruction algorithms are applied to two PPI networks and assessed using both global and local definitions of accuracy.LocPLproduces more accurate and biologically meaningful reconstructions on a versatile set of signaling pathways.LocPLis a powerful tool to automatically reconstruct signaling pathways from PPIs that leverages cellular localization information about proteins. The underlying dynamic program and signaling model are flexible enough to study cellular signaling under different settings of signaling flow across the cellular compartments.
Biofluid modeling of the coupled eye-brain system and insights into simulated microgravity conditions
Authors: Salerni Fabrizia , Repetto Rodolfo , Harris Alon , Pinsky Peter , Prud’homme Christophe , Szopos Marcela , Guidoboni Giovanna 
Published: April 2019

AbstractThis work aims at investigating the interactions between the flow of fluids in the eyes and the brain and their potential implications in the development of visual impairment in astronauts, a condition also known as spaceflight associated neuro-ocular syndrome (SANS). To this end, we propose a reduced (0-dimensional) mathematical model of fluid flow in the eyes and brain, which is embedded into a simplified whole-body circulation model. In particular, the model accounts for: (i) the flows of blood and aqueous humor in the eyes; (ii) the flows of blood, cerebrospinal fluid and interstitial fluid in the brain; and (iii) their interactions. The model is used to simulate variations in intraocular pressure, intracranial pressure and blood flow due to microgravity conditions, which are thought to be critical factors in SANS. Specifically, the model predicts that both intracranial and intraocular pressures increase in microgravity, even though their respective trends may be different. In such conditions, ocular blood flow is predicted to decrease in the choroid and ciliary body circulations, whereas retinal circulation is found to be less susceptible to microgravity-induced alterations, owing to a purely mechanical component in perfusion control associated with the venous segments. These findings indicate that the particular anatomical architecture of venous drainage in the retina may be one of the reasons why most of the SANS alterations are not observed in the retina but, rather, in other vascular beds, particularly the choroid. Thus, clinical assessment of ocular venous function may be considered as a determinant SANS factor, for which astronauts could be screened on earth and in-flight.
Evidence for Early European Neolithic Dog Dispersal: New Data on South-Eastern European subfossil dogs from Prehistory and Antiquity Ages
Authors: Marinov Miroslav , Yankova Iskra , Neov Boyko , Petrova Maria , Spassov Nikolai , Hristov Peter , Radoslavov Georgi 
Published: April 2019

AbstractObjectivesThe history of dog domestication is still under debate, but doubtlessly, it is a process of an ancient partnership between dogs (Canis familiaris) and humans. Although data on ancient DNA dog diversity are scarce, it is clear that several regional dog populations had been formed in Eurasia up to the Holocene. During the Neolithic Revolution and the transition from hunter-gatherer to farmer societies, followed by civilization changes in the Antiquity period, the dog population structure also changed. This process was due to replacement with newly formed dog breeds.MethodsIn this study we have presented for the first time mitochondrial data about South-Eastern Europe (the Balkans) ancient dog remains from the Early Neolithic (8 000 years BP) to the Late Antiquity ages (up to 3th century AD). A total of 25 samples were analyzed using the mitochondrial D-loop region (HVR1).ResultsThe results have shown the presence of A (70%) and B (25%) clades throughout the whole investigated period. In order to clarify the position of our results within the ancient dog population in Eneolithic Eurasia, we performed phylogenetic analysis with the available genetic data sets. This data revealed a similarity of the Bulgarian dogs’ structure to that of ancient Italian dogs (A, B, and C clades), which suggests a new prehistoric and historic Mediterranean dog population. A clear border can be seen between South-European genetic dog structure, on the one hand, and, on the other hand, Central-West (clade C), East (clade D) and North Europe (clades A and C). This corresponds to genetic data for European humans during the same period without admixture between dog populations.ConclusionsOur data have shown for the first time the presence of clade B in ancient Eurasia. This is not unexpected as the B haplogroup is widely distributed in extant Balkan dogs and wolves. The presence of this clade both in dogs and in wolves on the Balkans may be explained with hybridization events before the Neolithic period. The spreading of this clade across Europe together with the A clade is related to the possible dissemination of newly formed dog breeds from Ancient Greece, Thrace and the Roman Empire.
Promotion of adipogenesis by JMJD6 requires the AT hook-like domain and is independent of its catalytic function
Authors: Reyes-Gutierrez Pablo , Carrasquillo-Rodríguez Jake W. , Imbalzano Anthony N. 
Published: April 2019

AbstractJMJD6 is a member of the Jumonji C domain containing enzymes that demethylate and/or hydroxylate substrate proteins. It is a multi-functional protein that has been implicated in disparate aspects of transcriptional and post-transcriptional control of gene expression, including but not limited to enhancer and promoter binding, release of paused RNA polymerase II, control of splicing, and interaction with the translation machinery. JMJD6 contributes to multiple aspects of animal development, including adipogenesis modeled in culture. We mutated proposed or characterized domains in the JMJD6 protein to better understand the requirement for JMJD6 in adipogenic differentiation. Mutation of JMJD6 amino acids that mediate binding of iron and 2-oxogluterate, which are required cofactors for enzymatic activity, had no impact on JMJD6 function, showing that catalytic activity is not required for JMJD6 contributions to adipogenic differentiation. In addition, we documented the formation of JMJD6 oligomers and showed that catalytic activity is not required for oligomerization, as has been reported previously. We also observed no effect of mutations in the sumoylation site and in the poly-serine stretch. In contrast, mutation of the AT hook-like structure, which mediates interaction with DNA and/or RNA, compromised JMJD6 function. The ability of JMJD6 to interact with nucleic acids may be a critical requirement for its function in adipogenic differentiation. The requirement for the AT hook-like domain and the lack of requirement for catalytic activity giving rise to the idea that JMJD6 may be functioning as a scaffold protein that supports the interactions of other critical regulators.
Epidemiology of drug-resistant tuberculosis in Chongqing, China: a retrospective observational study from 2010 to 2017
Authors: Wu Bo , Yu Ya , Du Changting , Liu Ying , Hu Daiyu 
Published: April 2019

AbstractChina is one of the top 30 countries with high multidrug-resistant tuberculosis (MDR-TB) and rifampin-resistant tuberculosis (RR-TB) burden. Chongqing is a southwest city of China with a large rural population. A retrospective observational study has been performed based on routine tuberculosis (TB) surveillance data in Chongqing from 2010 to 2017. The MDR/RR-TB notification rate increased from 0.03 cases per 100,000 population in 2010 to 2.09 cases per 100,000 population in 2017. The extensively drug-resistant TB (XDR-TB) notification rate has increased to 0.09 cases per 100,000 population in 2017. There was a decreasing detection gap between the number of notified MDR/RR-TB cases and the estimate number of MDR/RR-TB cases among all notified TB cases. The treatment success rate of MDR/RR-TB was 50.66% in this period. The rate of MDR/RR-TB in new TB cases was 6.23%, and this rate in previously treated TB cases was 32.7%. Despite the progress achieved, the prevalence of MDR/RR-TB was still high facing challenges including detection gaps, the regional disparity, and the high risk for MDR/RR-TB in elderly people and farmers. Sustained government financing and policy support should be guaranteed in the future.
The role of client participation and psychological comfort in driving perceptions of audit quality
Authors: Sampet Jomjai , Sarapaivanich Naruanard , Patterson Paul 
Published: April 2019

Purpose
With increased competition under the establishment of the ASEAN Economic Community (AEC) in December 2015, CPA and Tax Auditors are free to export their services within AEC partner countries. Hence, it is crucial that the growing numbers of auditors in the region differentiate themselves by providing superior perceived audit quality and client value in order to retain (and attract) clients. Based on theoretical foundations of service-dominant logic and culture theory, the purpose of this paper is to investigate the role of client involvement in the audit process and client psychological comfort in influencing client perceptions of audit quality.

Design/methodology/approach
A survey was used to collect data from firms listed on the Thai Stock Exchange. The unit of analysis was the client firm. A key informant method was used whereby a senior manager, heavily involved in the auditor assessment and selection process answered all questions on behalf of their company. Data from 190 firms are subsequently analyzed with structural equation modeling.

Findings
Both client level of involvement and psychological comfort impact their perceptions of three dimensions of audit quality: service quality, independence and competence. Audit quality in turn is strongly associated with overall client satisfaction.

Originality/value
While various scholarly works have examined audit quality, this study does so in an emerging, highly collectivist culture (Thailand) where due to cultural norms, relationships take on added importance. More importantly, for the first time, the study shines the spotlight on the role that client involvement in the audit process, and client psychological comfort, play in influencing client perceptions of audit quality.
Could sustainability improve the promotion of luxury products?
Authors: Dekhili Sihem , Achabou Mohamed Akli , Alharbi Fatmah 
Published: April 2019

PurposeThis paper aims to examine the extent to which sustainability information unfavorably impacts consumers’ behavior in the case of luxury. In particular, it explores the effect of social and environmental attributes on the perceived quality.Design/methodology/approachA between-subject experimental design involving 973 French and Saudi consumers has been conducted.FindingsThe results indicate that sustainability information negatively impacts the perceived quality of luxury products. However, this result varies regarding the consumers’ country of origin. While no significant effect was observed in the case of French respondents, Saudi consumers lower the evaluation of quality when social information is provided. In addition, the negative effect of sustainability information is moderated by the consumers’ degree of liking of luxury and by the brand corporate social responsibility image.Research limitations/implicationsThis research fills a gap occurred in the previous literature. In effect, limited studies examined perceptions of the association between luxury and sustainability. In addition, it enriches the limited literature on sustainable consumption in the context of developing countries. However, further studies should focus on specific dimensions of quality and examine different sustainable practices and luxury goods.Practical implicationsFrom a practical point of view, this study suggests new applications with respect to the link between luxury and sustainability.Originality/valueNo study to date, as per the authors’ knowledge, has investigated empirically the impact of sustainability information on the perceived quality of luxury products. Contrary to the literature indicating a positive effect of sustainable attributes on consumers’ behavior, this study confirms the incompatibility between luxury and sustainability.
The moderating effect of corporate reputation on inter-firm alliance impact on company performance
Authors: Liu Xiaoyu , Vredenburg Harrie , Daellenbach Urs 
Published: April 2019

PurposeThe purpose of this paper is to untangle the impacts of a firm’s corporate reputation and its alliance partners’ social capital on its financial performance, drawing on the relational and the network points of view.Design/methodology/approachThis paper explored the moderating effect of corporate reputation on the relationship between partners’ social capital (e.g. resource heterogeneity, structural relations and partners’ social ties) and a focal firm’s performance. An OLS three-step regression model (controls, main effects and interaction effects) was used to test the proposed hypotheses based on 265 US joint ventures.FindingsThe influence of partners’ social capital on a focal firm’s performance is negatively moderated by the focal firm’s reputation at the firm and network levels; larger and more prestigious firms listed inFortunedatabase tend to choose partners with a higher level of resource heterogeneity, whereas smaller firms tend to choose partners in similar industries to increase economies of scale. The social capital factors of the partners will have different effects on the focal firm performance.Originality/valueThe value of this paper is in providing insight into the importance and nuances of corporate reputation in offsetting the advantages of inter-firm alliances and their impact on firm performance. In particular, the performance benefits of inter-firm alliance partners’ social ties and heterogeneous resources are negatively affected by the corporate reputation of a firm.
Development and validation of a measurement scale of the innovative culture in work teams
Authors: Solís Martín , Mora-Esquivel Ronald 
Published: April 2019

PurposeThis study aims to develop and validate a scale to measure innovative culture in work teams of the public sector (ICT).Design/methodology/approachA mixed research design was followed. An initial literature review and a qualitative study identify the possible dimensions that give content to the construct of the innovative culture. Subsequently, two quantitative studies are carried out to explore the measurement scale and provide evidence of its reliability and validity.FindingsThe results show that the ICT construct is composed of ten dimensions and likewise, exhibit internal consistency and evidence of validity.Originality/valueThis study attempts to provide an additional contribution to the conceptualization and measurement of innovative culture, through the development of a scale that consolidates the different dimensions proposed by the authors. Besides, the scale developed is the only one, as far as we know, that has been created to evaluate innovative culture in work teams.
Financial intermediation analysis from financial flows
Authors: De Moraes Claudio Oliveira , Antunes José Americo Pereira , Rodrigues Adriano 
Published: April 2019

Purpose
The purpose of this paper is to analyze the financial friction effect of non-performing loans (NPLs) on financial intermediation (FI) through empirical evidence from the Brazilian experience.

Design/methodology/approach
The authors develop a new variable, financial intermediation flow and a new indicator, FI, both measures of FI. To empirically test FI, the authors use a dynamic panel data framework that draws on 101 banks (December 2000 to December 2015).

Findings
An increase in NPL reduces FI. Thus, NPL amplifies financial friction in FI. This result holds in different time frames, such as the pre-crisis period, the crisis period and the post-crisis period.

Practical implications
The FI measure developed in this study offers the policymakers a possibility to monitor financial stability.

Originality/value
This study adds to this debate by proposing a measure of FI derived from financial flows. This measure allows one to estimate the role of NPL as a financial friction that can pose a threat to financial stability.
The importance of sources of meaning in life of community dwelling psychologically frail older people
Authors: Hoeyberghs Lieve Josée , Verté Emily , Verté Dominique , Schols Jos M.G.A. , De Witte Nico 
Published: April 2019

Purpose
Psychological frailty adds most to overall feelings of frailty, but is often neglected, although meaning in life is important for psychological well-being. The purpose of this paper is to explore the sources of meaning in life within psychologically frail older people.

Design/methodology/approach
Data (n= 16,872) generated from the Belgian Ageing Studies were collected, using the Comprehensive Frailty Assessment Instrument and the Sources of Meaning Profile (SOMP-R) instrument. Psychometric properties of the SOMP-R were explored using factor and reliability analysis and one-way-ANOVA analysis were used to asses mean differences.

Findings
Financial security, meeting basic needs and personal relations play an important role as sources of meaning in life. Moreover, the SOMP-R showed excellent psychometric properties.

Research limitations/implications
Due to the cross-sectional design of this study, evolution in time and causal links could not be assessed.

Practical implications
The findings of this study emphasize that sources of meaning in life are relevant and can be assessed using the SOMP-R upon which individually tailored care plans can be developed. The results show that, meaning in life as such plays an important role for psychologically frail older people. As a consequence, this offers insights to support these older people. Caregivers and policymakers might therefore take these results into account. Guarantee and/or follow up a frail individual’s financial security, assessing and enabling one’s personal relationships and meeting their basic needs are very important when taking care of psychologically frail older individuals.

Social implications
Besides the practical implications, the social inclusion of psychologically frail older people seems to be relevant.

Originality/value
To the best of the authors’ knowledge, the association between meaning in life and psychological frailty in later life is not yet investigated. Further the findings of this study emphasize that sources of meaning in life are relevant and can be assessed using the SOMP-R upon which individually tailored care plans can be developed.
Patient preference for biologic treatments of psoriasis in Japan
Authors: Tada Yayoi , Ishii Kanako , Kimura Junko , Hanada Keigo , Kawaguchi Isao 
Published: April 2019

AbstractPsoriasis is a chronic autoimmune disease affecting skin which may also manifest in nails and joints. Several biologic treatments have been approved in Japan for psoriasis. Each biologic has a different profile for efficacy and safety, including different dosing regimens and co‐payment considerations which may complicate treatment decisions made by patients and physicians during short consultations. Elucidating patient preference is expected to contribute to shared decision‐making between patients and physicians to optimize treatment satisfaction and outcomes. However, the number of studies investigating this in Japan is very limited. The study used a discrete choice experiment methodology to elicit patient preferences for hypothetical options in an experimental framework. Participants were asked to choose their preferred treatment option from two hypothetical choices, defined by different levels of six attributes (i.e. early onset of efficacy, long‐term efficacy, sustained efficacy after drug withdrawal, dosing convenience, co‐payment and risk of serious infection). The survey included 16 treatment choice scenarios and was completed by 395 participants. Across all participants, the attribute regarded as most important was sustained efficacy after drug withdrawal, followed by dosing convenience, co‐payment, long‐term efficacy, early onset of efficacy and risk of serious infection. The study found that patients prefer treatments which have durable efficacy and lower treatment burden characterized as fewer injection frequency and lower co‐payment. These results may be helpful to understand patient preference for biologic treatments used for psoriasis in Japan and contribute to shared decision‐making between patients and physicians to improve patient satisfaction and treatment outcomes.