bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing Loop Diuretic Efficacy and Safety in Patients with Acute Myeloid Leukemia and Differentiation Syndrome
Authors: Nethagani Sanjana , Fudacz Paul , Furner Mark , Rizwan Umer 
Published: December 2024

Background: Differentiation syndrome (DS) is a group of life-threatening complications associated with acute myeloid leukemia (AML) treatment. Loop diuretics are used to manage fluid overload and electrolyte imbalances in DS. The optimal choice of loop diuretics is unclear. Previous studies showed bumetanide and furosemide have similar efficacy and safety in patients with congestive heart failure. Recent research suggest torsemide may be non-inferior to furosemide in critically ill patients with acute kidney injury.
Methods: We performed a retrospective analysis of 5,670 patients diagnosed with AML and differentiation syndrome from a large federated network database. Patients were treated with furosemide (n=2,431), bumetanide (n=466), or torsemide (n=206), alongside glucocorticoids and allopurinol. Propensity score matching was utilized to control for confounding variables, including age, sex, and comorbidities such as chronic kidney disease, diabetes mellitus (types 1 and 2) and hypertension. Patients were matched by AML subtype, including acute myeloid leukemia and acute promyelocytic leukemia.
Results: No significant differences were seen in overall mortality or the incidence of acute renal failure among the three cohorts. Mean glomerular filtration rate, serum creatinine, blood urea nitrogen, serum uric acid, and serum potassium, did not differ significantly between the loop diuretic groups. However, a lower risk of hemodialysis utilization was observed in patients receiving furosemide compared to bumetanide (risk ratio 0.584, 95% CI 0.414-0.825).
Conclusion: Our findings suggest that furosemide, bumetanide, and torsemide have comparable efficacy and safety profiles in patients with AML and differentiation syndrome. Notably, furosemide was associated with a lower rate of hemodialysis utilization compared to bumetanide, with no significant difference in renal function or mortality. This may be due to bumetanide's higher potency, rapid absorption, and shorter half-life, potentially increasing the risk of electrolyte imbalances and acute kidney injury. Conversely, furosemide may facilitate greater cumulative sodium excretion in patients with chronic renal insufficiency.
Development of a PMGDNI model to predict the probability of three-month unfavorable outcome acute ischemic stroke after endovascular treatment: a cohort study
Authors: Yang Chao , Wang Jingying , Zhang Ruihai , Lu Yiyao , Hu Wei , Yang Peng , Jiang Yiqing , Hong Weijun , Shan Renfei , Xu Yinghe , Jiang Yongpo 
Published: December 2024

Abstract
Background
Patients with acute large vessel occlusion stroke (ALVOS) may exhibit considerable variability in clinical outcomes following mechanical thrombectomy (MT). This study aimed to develop a novel statistical model predicting functional independence three months post-endovascular treatment for acute stroke and validate its performance within the cohort.

Method
Consecutive patients undergoing endovascular treatment for acute stroke with large vessel occlusion were randomly divided into a modeling group and a validation group in a 7:3 ratio. Independent risk factors were identified through LASSO regression and multivariate logistic regression analyses, leading to the development of a prognostic model whose performance was rigorously validated.

Results
A total of 913 patients were screened, with 893 cases included. The modeling group comprised 625 cases, and the validation group included 268 cases. Identified independent factors for adverse outcomes after endovascular treatment of acute ischemic stroke (AIS) were pneumonia (OR = 4.517, 95% CI = 2.916–7.101, P < 0.001), mechanical ventilation (OR = 2.449, 95% CI = 1.475–5.148, P = 0.001), admission GCS ≥ 8 (OR = 0.365, 95% CI = 0.167–0.745, P = 0.008), dysphagia (OR = 2.074, 95% CI = 1.375–3.126, P < 0.001), and 72-hour highest Na ≥ 145 (OR = 2.794, 95% CI = 1.508–5.439, P = 0.002), along with intracranial hemorrhage (OR = 2.453, 95% CI = 1.408–4.396, P = 0.002). These factors were illustrated in a PMGDNI column chart. The area under the ROC curve for the modeling group was 82.5% (95% CI = 0.793–0.857), and for the validation group, it was 83.7% (95% CI = 0.789–0.885). The Hosmer-Lemeshow test indicates that there is no statistically significant difference (P > 0.05) between the predicted and actual probabilities of adverse outcomes. The clinical decision curve demonstrated optimal net benefits at thresholds of 0.30-1.00 and 0.25-1.00 for both training and validation sets, indicating effective clinical efficacy within these probability ranges.

Conclusion
We have successfully developed a new predictive model enhancing the accuracy of prognostic assessments for acute ischemic stroke following EVT. It provides an individual, visual, and precise prediction of the risk probability of a 90-day unfavorable outcome.
Lessons learned for pandemic preparedness in the neurodegenerative research and clinical fields: an advice report based on Parkinson’s disease as an example
Authors: Splinter Marije J. , Henderson Emily J. , Ben-Shlomo Yoav , Darweesh Sirwan K. L. , Sowa Pawel , Wolters Frank J. , Velek Premysl , Meijerink Hannie J. E. M. , Bakx Paulus , Ikram M. Arfan , de Schepper Evelien I. T. , Ikram M. Kamran , Licher Silvan 
Published: December 2024

Abstract
Background
A sustainable pandemic preparedness strategy is essential to ensure equitable access to healthcare for individuals with neurodegenerative diseases. Moreover, it is vital to provide clinicians and researchers in the neurodegenerative disease fields with resources and infrastructure to ensure continuity of their work during a (health) crisis.

Methods
We established an international collaboration between researchers, clinicians, and patient representatives from the Netherlands, Poland, and the United Kingdom. We co-created a pandemic preparedness plan primarily informed by examples from those affected by or working in the field of Parkinson’s disease, with potential application to other neurodegenerative diseases or the general population. This plan builds upon insights and experiences from four population-based studies during the COVID-19 pandemic. Between March and November 2023, we organised two hybrid meetings in Bristol (United Kingdom) and Rotterdam (the Netherlands), and two online meetings.

Results
Research recommendations included three core factors in questionnaire design during health crises: 1) using existing, validated questions, 2) questionnaire adaptability and flexibility, and 3) testing within and outside the research group. Additionally, we addressed burden of participation, and we advocated for robust data sharing practices, underlining the importance of regulatory measures extending beyond the COVID-19 pandemic. We also shared clinical perspectives, including strategies to mitigate social isolation; challenges in virtual versus in-person consultations; and systemic changes to recognise and prevent moral injury in healthcare professionals.

Conclusion
In this pandemic preparedness plan, we provide research and clinical recommendations tailored to the field of Parkinson’s disease, with broader relevance to other neurodegenerative diseases and the general population. This establishes an essential framework for setting up new studies and safeguarding research and clinical practices when a new pandemic or other (health) crisis emerges.

Graphical Abstract
Investigation of awareness, sanitation, and customer education practices among employees of pet and animal feed stores that sell live animals in the United States
Authors: Lord Jennifer , Pugh Sheri , Thompson Sharon R. 
Published: December 2024

Abstract
Background
Numerous zoonotic disease outbreaks have been associated with companion animals and poultry purchased at pet and animal feed stores. Employees are often the initial source of information for customers purchasing a new pet. Therefore, the objectives of this study were to: (1) investigate awareness, sanitation, and customer education practices related to zoonotic disease risk, and (2) identify predictors of providing customer education among employees of pet and animal feed stores that sell live animals.

Methods
A survey of pet and animal feed store employees was conducted to evaluate sanitation practices, training, and awareness of zoonotic disease risk. Differences in proportions of categorical variables were assessed using Chi-square tests. Wilcoxon rank-sum and Kruskal–Wallis tests were used to assess for differences in the values of ordinal variables based on the values of categorical variables. A partial proportional odds model was used to identify predictors of providing customer education.

Results
Surveys were completed by 206 respondents from the Southeast, Midwest, Southwest, and Western US, 146 of whom reported that their workplace sold live animals. Handwashing was more frequent among employees whose workplace had handwashing policies related to handling animals and their habitats (p < 0.001). Perceived zoonotic disease risk was higher among those who had received workplace training (p = 0.007). Higher odds of providing customer education related to zoonotic disease risk were associated with serving in a supervisory role (p = 0.005), higher perceived zoonotic disease risk (p = 0.001), and more frequent sanitation practices (handwashing, p = 0.031; surface disinfection, p < 0.001; and glove use, p < 0.001).

Conclusions
Pet and animal feed stores play an important role in minimizing occupational health hazards for employees and providing education for customers. These retailers should implement clear biosecurity protocols and provide training about zoonotic disease risk associated with handling live animals.
Arthroscopic anterior cruciate ligament reconstruction with and without tourniquet use: an updated systematic review and meta-analysis on clinical outcomes
Authors: Samei Mahdieh , Daliri Mahla , Sadeghi Masoumeh , Ganji Reza , Parsa Ali , Ebrahimzadeh Mohammad H. 
Published: December 2024

Abstract
Background
The use of a tourniquet is common during anterior cruciate ligament (ACL) reconstruction, offering convenience for the surgical procedure. However, the potential adverse effects of tourniquet use have gained increasing attention from clinical researchers. We conducted this systematic review and meta-analysis to compare the clinical outcomes of tourniquet application versus non-tourniquet approach during arthroscopic ACL reconstruction.

Methods
A comprehensive search of PubMed, Web of Science, Embase, and Cochrane Library databases, was performed through March 2023 to identify controlled clinical trials. The main outcomes assessed included post-operative drain output, post-operative pain using a visual analogue scale (VAS), operation time, calf girth, and thigh girth. A random-effects meta-analysis was performed to account for heterogeneity, with weighted mean difference (WMD) and 95% confidence intervals (CI) used as pooled estimates for clinical outcomes.

Results
Of the nine potentially related studies, seven eligible studies (sufficient quantitative data) were included in the meta-analysis. Postoperative drain output in the tourniquet group was on average 100 ml higher than in the non-tourniquet group (95% CI: 36 to 168). Pain, measured by the VAS at 24 h postoperatively, was 0.42 points higher in the tourniquet group (95% CI: 0.08 to 0.76), with the increase persisting at 48 h, averaging 0.40 points (95% CI: 0.12 to 0.69). Thigh girth in the tourniquet group was reduced by 1.8 cm (95% CI: -2.7 to -0.94). No significant differences were observed for calf girth and the operation time.

Conclusion
Our meta-analysis indicates that tourniquet use during arthroscopic ACL reconstruction is associated with higher pain levels, increased postoperative drain output, and reduced thigh girth. However, performing the surgery without a tourniquet does not significantly extend the operation time.

Trial registration
The protocol was registered in the International Prospective Register of Systematic Reviews, PROSPERO (CRD42023417604).
A qualitative exploration of the coordinator’s role in an intersectoral childhood overweight prevention programme in the Netherlands: ‘a lot is expected from one person’
Authors: Huiberts Irma , Slot-Heijs Jorien , Singh Amika , Collard Dorine 
Published: December 2024

Abstract
Background
Intersectoral collaboration and its coordination are vital for community health promotion. Given the diverse organisational contexts in which local coordinators build intersectoral collaboration, training and support needs of coordinators may vary widely. To date, there is limited insight into how coordinators tasked with building intersectoral collaboration apply their role given their specific organisational context. A more detailed understanding of this process will provide valuable guidance for training and supporting local coordinators. In the current study we focussed on coordinators involved in building intersectoral collaboration within the ‘Healthy Youth, Healthy Future’ (JOGG) approach in the Netherlands. The organisational contexts in which these local coordinators operate vary considerably. The aim of this study was to explore how local coordinators of the JOGG approach apply their role in building intersectoral collaboration and the competencies they require, taking into account their organisational context.

Methods
We conducted interviews with twelve local JOGG coordinators and two focus groups with eight community advisors from the national JOGG organisation. Data was analysed both inductively and deductively.

Results
JOGG coordinators appeared to take on seven different roles over time: implementer, project manager, networker, matchmaker, linchpin, politician and programme manager. These roles required different competencies and varied according to the organisational context, including: available resources, the position of the employing organisation in existing local stakeholder networks, the coordinators’ responsibilities in their employing organisation and professional background. In addition, the coordinators role depended on the implementation phase of the JOGG approach. During the first phase, roles at the operational level were more prominent, since they were important for engaging stakeholders and facilitating collaboration. In later phases, coordinators took on roles at a tactical and strategic level in order to mobilise their network.

Conclusions
This study highlights the diversity in how JOGG coordinators apply their role. Our findings highlight the importance of carefully selecting the organisation where coordinators are employed, as this influences their possibilities. In addition, our study provides directions for recruiting coordinators for an intersectoral health promotion approach and supporting them, taking into account the phase of the approach and organisational context.
Tailored culture strategies to promote antimicrobial secondary metabolite production in Diaporthe caliensis: a metabolomic approach
Authors: Hoyos Laura V. , Vasquez-Muñoz Luis E. , Osorio Yuliana , Valencia-Revelo Daniela , Devia-Cometa Daiana , Große Miriam , Charria-Girón Esteban , Caicedo-Ortega Nelson H. 
Published: December 2024

Abstract
Background
In the search for new antimicrobial secondary metabolites of fungi, optimizing culture conditions remains a critical challenge, as standard laboratory approaches often result in low yields. While non-selective methods, such as modifying culture media, have been effective in expanding the chemical diversity of fungal metabolites, they have not yet established a direct link to key process parameters crucial for further optimization. This study investigates the capacity of Diaporthe caliensis as a biofactory for biologically active secondary metabolites, employing tailored culture media to explore the relationship between chemical diversity and critical process variables.

Results
The metabolomic profiles, antibacterial activities, and production yields of the extracts were analyzed to progressively adjust the culture conditions. This study was conducted in five steps, evaluating carbon and nitrogen source concentration, nitrogen source type, salt supplementation, and pH adjustment. Altering the rice starch concentration affected biomass yield per unit of oxygen consumed, while modifications to the nitrogen source concentration influenced both the bioactivity and chemical space by Diaporthe caliensis. Despite changes at the metabolome level, the extracts consistently exhibited potent antibacterial activities, influenced by the nitrogen source, added salts and pH adjustments. For instance, when using corn steep liquor and rice starch, supplemented with micronutrients, different metabolites were produced depending on whether buffer or water was used, though both conditions showed similar antibacterial activities (IC50 ≈ 0.10 mg mL− 1 against Staphylococcus aureus and ≈ 0.14 mg mL− 1 against Escherichia coli). In the treatment where buffer was used to stabilize pH change, there was an increase in the production of phomol-like compounds which are associated with known antibiotic properties. In contrast, in the treatments using water, the drop in pH stimulated the production of previously unidentified metabolites with potential antimicrobial activity.

Conclusions
This study proposes a strategic methodology for the tailored formulation of culture media aiming to promote the biosynthesis of diverse secondary metabolites. This approach revealed the critical role of nutrient limitation and pH regulation in stimulating the production of polyketide-lactone derivatives, including the antibiotic phomol. Ultimately, the systematic, custom-designed culture conditions developed in this work offer a promising strategy for expanding the chemical diversity of Diaporthe caliensis, while providing valuable insights into the key parameters needed for optimizing this fungal biofactory.

Graphical Abstract
Exploring the innovative application of cerium oxide nanoparticles for addressing oxidative stress in ovarian tissue regeneration
Authors: Lakshmanan Maya , Saini Monika , Nune Manasa 
Published: December 2024

AbstractThe female reproductive system dysfunction considerably affects the overall health of women and children on a global scale. Over the decade, the incidence of reproductive disorders has become a significant source of suffering for women. Infertility in women may be caused by a range of acquired and congenital abnormalities. Ovaries play a central role in the female reproductive function. Any defect in the normal functioning of these endocrine organs causes health issues and reproductive challenges extending beyond infertility, as the hormones interact with other tissues and biological processes in the body. The complex pathophysiology of ovarian disorders makes it a multifactorial disease. The key etiological factors associated with the diseases include genetic factors, hormonal imbalance, environmental and lifestyle factors, inflammatory conditions, oxidative stress, autoimmune diseases, metabolic factors, and age. Oxidative stress is a major contributor to disease development and progression affecting the oocyte quality, fertilization, embryo development, and implantation. The choice of treatment for ovarian disorders varies among individuals and has associated complications. Reproductive tissue engineering holds great promise for overcoming the challenges associated with the current therapeutic approach to tissue regeneration. Furthermore, incorporating nanotechnology into tissue engineering could offer an efficient treatment strategy. This review provides an overview of incorporating antioxidant nanomaterials for engineering ovarian tissue to address the disease recurrence and associated pathophysiology. Cerium oxide nanoparticles (CeO2 NPs) are prioritized for evaluation primarily due to their antioxidant properties. In conclusion, the review explores the potential applications of CeO2 NPs for effective and clinically significant ovarian tissue regeneration.
Graphical Abstract
Effects of maternal feeding of clofibrate on hepatic fatty acid metabolism in suckling piglet
Authors: Zhao Jinan , Pike Brandon , Wang Feng , Yang Lin , Meisner Paige , Huang Yanling , Odle Jack , Lin Xi 
Published: December 2024

Abstract
Background
Energy deficiency is a leading cause of the high pre-weaning mortality of neonatal piglets in the swine industry. Thus, optimal energy metabolism is of crucial importance for improving the survivability of neonatal piglets. The effective utilization of milk fat as primary energy is indispensably required.

Methods
Pregnant sows (n = 27) were randomly assigned into 3 treatments. Each treatment received a standard diet (3,265 kcal ME/kg) supplemented with either 0, 0.25% or 0.5% clofibrate (w/w) from d 107 of gestation to d 7 of lactation. The effects of maternal clofibrate on their milk fatty acid (FA) and performance of the piglets were evaluated. The evaluations were performed via measuring sow productive performance, milk FA composition, and hepatic FA oxidation of the piglets at birth and d 1, 7, 14 and 19 after birth.

Results
Maternal supplementation of clofibrate had no effect on reproductive performance of the sows at farrowing and weaning (P > 0.05). However, the mortality at weaning was reduced for piglets from sows with 0.25% of clofibrate, and the average weekly (and daily) gain was higher in piglets from sows that received clofibrate than sows without clofibrate in the first week (P < 0.0001). Maternal clofibrate increased percentage of milk C12:0 and C14:0 FAs but decreased C18:2 and n-6 polyunsaturated FAs. Maternal clofibrate also increased plasma ketone body levels and hepatic FA oxidation measured at the first day of birth, but the increase was not detected in piglets on d 7, 14 or 19. Clofibrate was not detected in milk collected from the clofibrate-treated sows. The percentage of FA oxidation decreased, and the percentage of FA esterification increased with increasing in postnatal age. Supplemental carnitine increased FA oxidation regardless of succinate dehydrogenase inhibition, and the increase had no effect on FA esterification.

Conclusions
Maternal supplementation of clofibrate during late gestation and early lactation increases hepatic FA oxidative metabolism at birth and improves growth performance of newborn piglets. Maternal clofibrate transfer to suckling piglets via milk was not detected. Carnitine availability is critical for piglets to maintain a high FA oxidation rate during the suckling period.