bluuebunny/crossref_metadata_2025_split huggingface dataset:
Male sterility in soybean: Occurrence, molecular basis and utilization
Authors: Li Jiajia , Nadeem Muhammad , Sun Genlou , Wang Xiaobo , Qiu Lijuan 
Published: October 2019

AbstractIn plants, male sterility (MS) is a specific breeding target trait. With the advancements in agriculture, utilization of heterosis breeding in hybrid production through MS lines has become the main breeding tool of various cross‐pollinated and even self‐pollinated crops. Soybean is an essential source of oil and protein; however, the low yield is a major factor limiting its development. Soybean MS mainly comprises cytoplasmic‐nuclear MS and nuclear/genic MS (NMS/GMS), which can effectively utilize heterosis to improve soybean yield. This review outlines the recent research progress on the development of new genetically MS lines, exploring the underlying molecular mechanism of MS, identification and cloning of MS and fertility restoration genes, and the application of MS lines. We further discussed and prospected the future developmental scenario direction of the soybean MS, based on the previous studies of other crops sterility system. Moreover, this review also provides comprehensive information for better application of MS to soybean breeding programme.
Characterization of the Q.Ymym region on wheat chromosome 2D associated with wheat yellow mosaic virus resistance
Authors: Kobayashi Fuminori , Kojima Hisayo , Tanaka Tsuyoshi , Saito Mika , Kiribuchi‐Otobe Chikako , Nakamura Toshiki 
Published: October 2019

AbstractYellow mosaic disease, caused by wheat yellow mosaic virus (WYMV), is one of the most serious diseases of winter wheat in Japan and China. A single major QTL for WYMV resistance in the Japanese wheat variety 'Yumechikara', designated Q.Ymym, has been mapped on a 43.6 cM linkage block between the two markers Xcfd233 and Xgwm349 on chromosome 2D. We were able to obtain two recombinants within the block, which facilitated reducing the size of the linkage block. The pseudomolecule sequence of 'Chinese Spring' (CS) indicated that the original Q.Ymym region of 43.6 cM corresponded to 68.5 Mb and the narrowed Q.Ymym region represents a size of 27.3 Mb. The sequence features of the Q.Ymym region were unique in comparison with CS sequences, which may have led to the low recombination rate within the block. The Q.Ymym haplotype block was detected in other WYMV‐resistant varieties but not in the susceptible varieties used in this study. The unique sequence structure of the Q.Ymym region allowed the development of co‐dominant markers for use in marker‐assisted selection.
Monitoring trough levels in cyclosporine for atopic dermatitis: A systematic review
Authors: Blake Stephanie C. , Murrell Dedee F. 
Published: October 2019

AbstractBackgroundCyclosporine is a useful immunosuppressive agent for achieving disease control in moderate to severe atopic dermatitis in children and adults. However, it carries the potential for nephrotoxicity. Monitoring of drug levels is performed in other patient groups, such as transplant recipients, but is not commonplace in the management of atopic dermatitis.ObjectivesTo investigate levels of nephrotoxicity associated with cyclosporine use in atopic dermatitis and assess potential correlation with trough levels of cyclosporine.MethodsAn electronic search was conducted on MEDLINE, EMBASE, and Cochrane databases for randomized controlled trials and cohort studies assessing the safety profile of cyclosporine compared to placebo or other atopic dermatitis treatments, in adult and pediatric atopic dermatitis patients from 1966 to May 2019. Studies that did not assess renal toxicity were excluded from analysis.ResultsThirty‐eight trials were included for analysis, excluding 11 that did not assess renal toxicity. Descriptive statistical analysis only was performed, due to the high heterogeneity between study methodologies. Significant renal toxicity was seen in 0%‐9% of pediatric participants. Monitoring of trough cyclosporine levels was performed in only 10 of the studies, and their correlation to toxicity or disease activity was not explored.ConclusionThere is limited evidence in atopic dermatitis regarding trough level monitoring of cyclosporine. Currently, the practice is not commonplace, particularly in pediatrics, and this is reflected in trial methodology. Monitoring may be useful in specific pediatric groups, such as those on multiple concurrent medications, patients with hepatic or renal dysfunction and non‐responders to therapy.
Trends in the off‐label use of β‐blockers in pediatric patients
Authors: Kaley Vishal R , Aregullin E Oliver , Samuel Bennett P , Vettukattil Joseph J 
Published: October 2019

AbstractThe use of US Food and Drug Administration (FDA)‐approved drugs for the treatment of an unapproved indication or in an unapproved age group, or at doses or route of administration not indicated on the label is known as off‐label use. Off‐label use may be beneficial in circumstances when the standard‐of‐care treatment has failed, and/or no other FDA‐approved medications are available for a particular condition. In pediatric patients, off‐label use may increase the risk of adverse events as pharmacokinetic and pharmacodynamic data are limited in children. Approximately 73% of off‐label drugs currently prescribed for various conditions do not have sufficient scientific evidence for safety and efficacy. For example, β‐blockers are a class of drugs with FDA‐approval for very few indications in pediatrics but are commonly used for various off‐label indications. Interestingly, the proportion of off‐label use of β‐blockers in adults is at about 52% (66.2 million) of the total number of β‐blockers prescribed. The frequency of off‐label use of β‐blockers in children is also high with limited data on the indications as well as safety and efficacy. We present trends in off‐label use of β‐blockers in children to discuss drug safety and efficacy and include recommendations for pediatric providers.
Living donor liver transplantation in Alagille syndrome—Single center experience from south Asia
Authors: Valamparampil Joseph J. , Reddy Mettu Srinivas , Shanmugam Naresh , Vij Mukul , Kanagavelu Rathnavel Govindaraju , Rela Mohamed 
Published: October 2019

AbstractTo analyze the clinical characteristics and the outcomes of living donor liver transplantation in children with Alagille syndrome (AGS). Clinical data of children with AGS who underwent liver transplantation between July 2009 and May 2019 in our unit were retrospectively analyzed. Primary end‐points were patient and graft survival. Ten children with AGS underwent living donor liver transplantation at a median age of 28 months (range, 12‐84 months). Jaundice was the most common initial symptom and was noted after a median duration of 20 days after birth (range, 7‐60 days). Two patients had undergone Kasai porto‐enterostomy for misdiagnosis of biliary atresia. The most common indication for transplantation was severe pruritus with poor quality of life. Explant livers in three children showed cirrhosis with early well‐differentiated hepatocellular carcinoma. We have 100% patient and graft survival at a mean follow‐up of 32 months (range 3‐72 months). The median z‐score for weight and height at liver transplantation was −2.66 (range: −6.44 to −0.9) and −3.6 (range: −7.96 to −0.93) while at follow‐up was −1.7 (range: −3.4 to −0.35) and −2.1 (range: −3.9 to −1.4), respectively. The estimated glomerular filtration rate was normal pretransplant and follow‐up. This is the first series of LDLT for Alagille syndrome in the Indian sub‐continent. We report excellent post‐transplant outcomes in contrast to outcomes reported from Western literature.
Development of a culturally informed protocol for hair cortisol sampling in Black women
Authors: Manns‐James Laura , Neal‐Barnett Angela 
Published: October 2019

AbstractThis article describes the development of a culturally informed hair collection protocol for cortisol research with Black women. We first conducted a formative research study investigating the acceptability of hair cortisol sampling for African‐American college women, where acceptance reached 54%. Findings, including the reasons reported for participation, were then used to develop a culturally informed hair sampling protocol for two studies in a community setting where acceptance improved to 75%. Specific barriers to hair sampling for research in this population were supported. Recommendations to increase participation in hair sampling are provided.
Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm
Authors: Ramírez‐Vasquez Rafael , Cesari Andreina , Greco Micaela B. , Cano Adriana , Hozbor Federico 
Published: October 2019

AbstractSeminal plasma (SP) proteins interact with sperm plasma membrane (PM) modulating its functionality. It has been shown that SP proteins can reverse the damage caused by freeze‐thaw; however in these studies, SP has been added to washed sperm (i.e., cells depleted from homologous SP and extender). The aim of the current study was to assess whether the egg yolk‐based extender (EY) modifies SP ability to ameliorate sperm parameters in frozen‐thawed ram spermatozoa. Ejaculates were diluted in EY or soybean lecithin‐based extender (SL) and evaluated before and after freezing to measure the cell damage according to the extender. Even when all classical parameters decreased after freezing, as expected (p < .05), there was no effect of the extender. SP treatment was applied after freeze‐thaw. Sperm were incubated with SP (20% v/v) in the presence of either EY or SL, and sperm parameters were assessed after thawing compared with the same treatments after Percoll sperm selection (washed). Treatments with 20% SP improved sperm total and progressive motility compared with controls regardless of washing and extender (p < .05); however, washed sperm showed higher percentage of total sperm motility compared with those unwashed (p < .05). Moreover, treatment with 20% SP showed significantly higher percentages of PM integrity, sperm with intact acrosomes, integrity of chromatin and non‐capacitated sperm in samples diluted with EY when washed before treatment compared with the other conditions (p < .05). It was concluded that the presence of the extenders and particularly egg yolk alters the SP capacity to reduce the cryodamage.
A Dryland Cropping Revolution? Linking an Emerging Soil Health Paradigm with Shifting Social Fields among Wheat Growers of the High Plains
Authors: Rosenzweig Steven T. , Carolan Michael S. , Schipanski Meagan E. 
Published: October 2019

AbstractOnce reliant on year‐long periods of unvegetated fallow, dryland farmers are reaping environmental and economic benefits by replacing fallow with a crop, a practice called cropping system intensification. However, in the U.S. High Plains, transitions to intensified cropping systems have been slow relative to other regions, and cropping systems have stratified into varying degrees of intensity. Prior attempts to explain the wave of cropping system intensification have largely focused on simple economic rationales, and thus we lack a critical understanding of the social dynamics underlying the revolution in semi‐arid cropping systems. We examined the motivations, perceptions, and social interactions of dryland farmers that practice different levels of cropping system intensity in Colorado and Nebraska. Building on Carolan's application of Bourdieusian social fields to agriculture, we identify overlapping fields expressed among interviewees. While these fields are reflected in farms' different degrees of intensification, they can be used to help identify and locate farmers associated with the emerging soil health (or regenerative agriculture) movement. The paper concludes by identifying strategies for change, some which would serve to reshape social fields, and others which leverage existing social positions and relationships to enable farmers to overcome the barriers constraining cropping system intensification.
Collaboration for Sustainable Intensification: The Underpinning Role of Social Sustainability
Authors: Wynne‐Jones Sophie , Hyland John , Williams Prysor , Chadwick Dave 
Published: October 2019

AbstractSustainable Intensification (SI) has been popularised in recent years as an approach seeking to balance the potentially conflicting demands of enhancing agricultural outputs with reducing the negative impacts arising from the current food system. Proponents have argued that SI can benefit from collaboration between farmers, but understanding is limited by a lack of data on current collaborative practices. Questions have also been raised as to whether the SI agenda pays sufficient attention to social sustainability as part of a fully integrated conception of SI. Tackling these issues, this paper reports on mixed methods data collection from seven case areas across the UK, with a particular focus on the experience of upland livestock farmers in north Wales. We evidence: (1) The extent, forms and preferences associated with farmers’ collaboration; with findings demonstrating higher levels of collaboration than anticipated and a preference for informal forms of co‐working. (2) The underpinning and mutually reinforcing role of social interconnectedness in the delivery of diverse outcomes from collaboration. (3) How SI is perceived to threaten social sustainability, and thus work against a more integrated model of delivery. The paper concludes by arguing for a genuinely integrative model of SI to secure collaborations going forwards.
Soil fertility assessment and management from the perspective of farmers in four villages of eastern South Africa
Authors: Buthelezi‐Dube Nkosinomusa Nomfundo , Hughes Jeffrey Charles , Muchaonyerwa Pardon , Caister Karen Fern , Modi Albert Thembinkosi 
Published: October 2019

AbstractUnderstanding farmer perceptions of soil fertility is necessary for the development of appropriate assessment methods for sustainable agro‐ecosystems. This study investigated farmer perceptions of soil fertility and management in four villages of eastern South Africa. A questionnaire was administered to 50 farmers from each village to obtain a general overview of local soil knowledge as well as soil fertility perceptions and assessment. Ten farmers were then chosen from each village for in‐field walks and to gather in‐depth knowledge of local soil fertility concepts, soil‐crop associations and soil management. During in‐field walks, farmers were asked to identify fertile, moderately fertile and low soil fertility plots in their fields from which topsoil samples were taken for laboratory fertility analysis. Local soil fertility descriptors included crop performance and yield, soil texture, stoniness and consistence. Using these descriptors, farmers have developed specific soil use and management practices. There was generally poor agreement between farmer qualitative assessment and measured chemical fertility parameters. However, the study revealed that the generally ignored local qualitative soil knowledge of farmers could be linked to crop performance and potentially supports laboratory soil analysis in terms of its implication for smallholder agriculture in remote areas. The descriptors identified and overall assessment used by farmers in this study reflected considerable soil knowledge employed in daily decision‐making. Action learning and research that links local soil fertility descriptors to soil acidity and specific soil nutrient levels is thus recommended for effective identification of yield‐limiting factors for sustainable crop production in low‐input agriculture.
Gene duplication and transposition of mobile elements drive evolution of the Rpv3 resistance locus in grapevine
Authors: Foria Serena , Copetti Dario , Eisenmann Birgit , Magris Gabriele , Vidotto Michele , Scalabrin Simone , Testolin Raffaele , Cipriani Guido , Wiedemann‐Merdinoglu Sabine , Bogs Jochen , Di Gaspero Gabriele , Morgante Michele 
Published: October 2019

SummaryA wild grape haplotype (Rpv3‐1) confers resistance to Plasmopara viticola. We mapped the causal factor for resistance to an interval containing a TIR‐NB‐LRR (TNL) gene pair that originated 1.6–2.6 million years ago by a tandem segmental duplication. Transient coexpression of the TNL pair in Vitis vinifera leaves activated pathogen‐induced necrosis and reduced sporulation compared with control leaves. Even though transcripts of the TNL pair from the wild haplotype appear to be partially subject to nonsense‐mediated mRNA decay, mature mRNA levels in a homozygous resistant genotype were individually higher than the mRNA trace levels observed for the orthologous single‐copy TNL in sensitive genotypes. Allelic expression imbalance in a resistant heterozygote confirmed that cis‐acting regulatory variation promotes expression in the wild haplotype. The movement of transposable elements had a major impact on the generation of haplotype diversity, altering the DNA context around similar TNL coding sequences and the GC‐content in their proximal 5′‐intergenic regions. The wild and domesticated haplotypes also diverged in conserved single‐copy intergenic DNA, but the highest divergence was observed in intraspecific and not in interspecific comparisons. In this case, introgression breeding did not transgress the genetic boundaries of the domesticated species, because haplotypes present in modern varieties sometimes predate speciation events between wild and cultivated species.
Maximizing leaf carbon gain in varying saline conditions: An optimization model with dynamic mesophyll conductance
Authors: Qiu Rangjian , Katul Gabriel G. 
Published: October 2019

SUMMARY
While the adverse effects of elevated salinity levels on leaf gas exchange in many crops are not in dispute, representing such effects on leaf photosynthetic rates (A) continues to draw research attention. Here, an optimization model for stomatal conductance (gc) that maximizes A while accounting for mesophyll conductance (gm) was used to interpret new leaf gas exchange measurements collected for five irrigation water salinity levels. A function between chloroplastic CO2 concentration (cc) and intercellular CO2 concentration (ci) modified by salinity stress to estimate gm was proposed. Results showed that with increased salinity, the estimated gm and maximum photosynthetic capacity were both reduced, whereas the marginal water use efficiency λ increased linearly. Adjustments of gm, λ and photosynthetic capacity were shown to be consistent with a large corpus of drought‐stress experiments. The inferred model parameters were then used to evaluate the combined effects of elevated salinity and atmospheric CO2 concentration (ca) on leaf gas exchange. For a given salinity level, increasing ca increased A linearly, but these increases were accompanied by mild reductions in gc and transpiration. The ca level needed to ameliorate A reductions due to increased salinity is also discussed using the aforementioned model calculations.
Clinical impact of amotosalen‐ultraviolet A pathogen‐inactivated platelets stored for up to 7 days
Authors: Infanti Laura , Holbro Andreas , Passweg Jakob , Bolliger Daniel , Tsakiris Dimitrios A. , Merki Ramona , Plattner Alexandra , Tappe David , Irsch Johannes , Lin Jin‐Sying , Corash Laurence , Benjamin Richard J. , Buser Andreas 
Published: October 2019

BACKGROUNDUniversal pathogen inactivation of platelet concentrates (PCs) using amotosalen/ultraviolet A with 7‐day storage was implemented in Switzerland in 2011. Routine‐use data were analyzed at the University Hospital Basel, Switzerland.STUDY DESIGNA retrospective two‐cohort study of patient and PC characteristics, component usage, patient outcomes, count increments (CIs), and adverse events were analyzed for two consecutive 5‐year periods with either 0‐ to 5‐day‐old conventional PC (C‐PC) (n = 14,181) or 0‐ to 7‐day‐old pathogen‐inactivated PC (PI‐PC) (n = 22,579).RESULTSIn both periods, PCs were issued for transfusion on a “first in, first out” basis. With 7‐day PI‐PC, wastage was reduced from 8.7% to 1.5%; 16.6% of transfused PI‐PCs were more than 5 days old. Transfusion of PI‐PC more than 5 days old compared with 5 days old or less did not increase platelet and RBC use on the same or next day as an indirect measure of hemostasis and did not increase transfusion reactions. Mean corrected count increments (CCIs) for PI‐PC stored for 5 days or less were 22.6% lower than for C‐PC (p < 0.001), and declined with increasing storage duration for both, although the correlation was weak (r2 = 0.005‐0.014). Mean number of PCs used per patient and duration of PC support were not different for hematology/oncology, allogeneic and autologous hematopoietic stem cell transplant (HSCT), and general medical/surgical patients, who used the majority (~92.0%) of PI‐PCs. Five‐year treatment‐related mortality in allogeneic HSCT was unchanged in the PI‐PC period.CONCLUSIONSPI‐PCs with 7‐day storage reduced wastage and did not increase PC or red blood cell utilization or adverse reactions compared with fresh PI‐PC or a historical control group, demonstrating preserved efficacy and safety.
Freeze‐dried plasma stability under prehospital field conditions
Authors: Zur Meital , Glassberg Elon , Gorenbein Pavel , Epstein Elad , Eisenkraft Arik , Misgav Mudi , Avramovich Eva 
Published: October 2019

BACKGROUNDThis study evaluated the effect of routine, uncontrolled, Israeli field storage conditions on the stability and efficacy of Lyo‐Plas N freeze‐dried plasma (FDP). We evaluated clotting factors V, VIII, and XI; proteins S and C; fibrinogen; partial thromboplastin time (PTT); antithrombin III (ATIII); von Willebrand factor (VWF); and international normalized ratio (INR) in FDP stored at 4°C, 25°C, and 40°C for 6 and 12 months, as well as FDP returned from field units after uncontrolled storage for 15 months (manufacturer's shelf life).METHODS AND MATERIALSAfter reconstitution, clotting factor levels were compared to those of freshly supplied FDP doses.RESULTSAt 4°C for 12 months, factor V decreased slightly. At 25°C, average fibrinogen and factor V content were significantly lower at both periods, and INR was higher after 12 months. At 40°C, all samples were out of normal range in at least one clotting factor after 6 or 12 months. After field storage for 15 months, fibrinogen, factors V and XI, PTT, and protein S were significantly decreased, and INR increased. However, these levels were still within laboratory norms. Statistically significant difference in clotting factors compared to laboratory normal range was found in INR (higher) and factor V (lower).CONCLUSIONSOur data show minimal decreases in clotting factors in FDP after storage under field conditions, when compared to laboratory normal ranges. Along with the many advantages of FDP, this supports its use at the point of injury under battlefield conditions, despite uncontrolled storage environments. Under controlled storage conditions at 4°C, shelf life could possibly be extended, although further study is required.