bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gestational pertussis vaccination and the infant’s cellular immune response against whole-cell pertussis vaccine in the first year of life
Authors: Argondizo-Correia Carolina , Rehder de Andrade Vaz-de-Lima Lourdes , Uchima Uehara Elaine , Gatti Fernandes Eder , Keico Sato Helena , Sayuri Sato Ana Paula , José de Albuquerque Luna Expedito , Ayres de Castilho Euclides , Alves de Brito Cyro 
Published: July 2019

AbstractPertussis resurgence worldwide calls for new prevention strategies, as the recently incorporated vaccine booster dose during pregnancy, whose aim is to protect newborns from infection. In Brazil, maternal Tdap vaccination is recommended since 2014, and we reported that this strategy promotes high transplacental transfer of anti-PT IgG and it is effective in protecting infants early in life. Young children are the most susceptible group and with higher mortality rates, however, it is not well known whether the elicited anti-pertussis maternal antibodies could influence in the children’s immune responses further in life, especially after their own vaccination against pertussis. Considering this scenario, we conducted a study with children born to mothers who either received or not the booster dose during pregnancy, after their primary pertussis vaccination, in order to investigate the first impact of maternal immunisation on the response to infant immunisation regarding the cellular immune response, while comparing with data from the literature. As transfer of maternal antibodies could result in attenuation of the immune response to vaccination in infants, this study performed to determine whether higher levels of maternal antibodies could influence in the immune response of infants to the whole-pertussis vaccination series. Results showed no difference in cytokine production between groups, a first suggestion that maternal vaccination may not interfere with recognition and cellular response generation to vaccination. This data, together with humoral immunity and epidemiological studies, is important for the implementation of maternal immunisation strategies nationwide and will contribute to assure public policies regarding vaccination schemes.ImportancePertussis, or whopping cough, is a respiratory infectious disease caused by a bacterial agent, resulting in violent coughs and possibly death in vulnerable groups, such as young children and neonates. It is known that pregnant mothers transfer antibodies to their developing foetuses for protection in early life, however anti-pertussis antibodies are not highly detected in young children. Thus, a pertussis maternal vaccination was implemented to increase maternal anti-pertussis antibodies levels in pregnant women and therefore the transference to the foetus. However, maternal antibodies can also interfere in the child immune response in the first months of life. The significance of our research is in analysing the cellular immune response of children born from pertussis-vaccinated mothers, which will give a first glimpse on how maternal antibodies could modulate the child’s response to pertussis in early life.
Model Plasma Membrane exhibits a Microemulsion in both Leaves providing a Foundation for “Rafts”
Authors: Allender D. W. , Giang H. , Schick M. 
Published: July 2019

AbstractWe consider a model plasma membrane, one that describes the outer leaf as consisting of sphingomyelin, phosphatidylcholine, and cholesterol, and the inner leaf of phosphatidylethanolamine, phosphatidylserine, phosphatidyl-choline, and cholesterol. Their relative compositions are taken from experiment, and the cholesterol freely interchanges between leaves. Fluctuations in composition are coupled to fluctuations in the membrane height as in the Leibler-Andelman mechanism. Provided that the membrane is of relatively constant thickness, this coupling of fluctuations also provides a coupling between the composition fluctuations of the two leaves. Structure functions display, for components in both leaves, a peak at non-zero wavevector. This indicates that the disordered fluid membrane is characterized by structure on a scale given by membrane properties. From measurements on the plasma membrane, this scale is on the order of 100 nm. The theory provides a tenable basis for the origin of “rafts”.Statement of SignificanceThe hypothesis that the plasma membrane is not homogeneous, but rather is heterogeneous, with rafts” of one composition floating in a sea of another, has overturned conventional views of this membrane and how it functions. Proteins prefer either the raft or the sea, and so are not uniformly distributed. Hence they perform more efficiently. From experiment, rafts are thought to be about 100 nm. However there is no realistic model that provides: a length scale for the rafts; a raft in both leaves of the membrane; the composition of the raft. We provide such a model. In contrast to other theories, the raft and sea are distinguished not only by composition, but also by a difference in curvature.
Physiological basis of noise-induced hearing loss in a tympanal ear
Authors: Warren Ben , Fenton Georgina E , Klenschi Elizabeth , Windmill James FC , French Andrew S 
Published: July 2019

AbstractAcoustic overexposure, such as listening to music too loud and too often, results in noise-induced hearing loss. The pathologies of this prevalent sensory disorder begin in the synapses of the primary auditory receptors, their postsynaptic partners and supporting cells. The extent of noise-induced damage, however, is determined by over-stimulation of primary auditory receptors. When over-stimulated, an excessive amount of positive ions flood into the primary auditory receptors, triggering the activation of ion channels and possibly disrupting their ability to encode sound. A systematic characterisation of the electrophysiological function of primary auditory receptors is warranted to understand how noise-exposure impacts on downstream targets, where the pathologies of hearing loss begin. Here, we used the experimentally-accessible locust ear to characterise noise-induced changes in the auditory receptors. Although, we found a decrease in ability of the primary auditory neurons to encode sound, this is probably due to pathologies of their supporting cells.
Establishment and validation of an endoplasmic reticulum stress reporter to monitor zebrafish ATF6 activity in development and disease
Authors: Clark Eric M. , Nonarath Hannah J.T. , Bostrom Jonathan R. , Link Brian A. 
Published: July 2019

AbstractInduction of endoplasmic reticulum (ER) stress is associated with diverse developmental and degenerative diseases. Modified ER homeostasis causes activation of conserved stress pathways at the ER called the unfolded protein response (UPR). ATF6 is a transcription factor activated during ER stress as part of a coordinated UPR. ATF6 resides at the ER, and upon activation is transported to the Golgi apparatus where it is cleaved by proteases to create an amino-terminal cytoplasmic fragment (ATF6f). ATF6f translocates to the nucleus to activate transcriptional targets. Here, we describe establishment and validation of zebrafish reporter lines forATF6activity. These transgenic lines are based on a defined and multimerizedATF6consensus site which drives either eGFP or destabilized eGFP (d2GFP), enabling dynamic study ofATF6activity during development and disease. The results show that the reporter is specific for the ATF6 pathway, active during development, and induced in disease models known to engage UPR. Specifically, during development,ATF6activity is highest in the lens, skeletal muscle, fins, and gills. The reporter is also activated by common chemical inducers of ER stress including tunicamycin, thapsigargin, and brefeldin A, as well as by heat shock. In both an ALS and a cone dystrophy model,ATF6reporter expression is induced in spinal cord interneurons or photoreceptors, respectively, suggesting a role for ATF6 response in multiple neurodegenerative diseases. Collectively our results show theseATF6reporters can be used to monitorATF6activity changes throughout development and in zebrafish models of disease.Summary StatementWe have established and validated transgenic zebrafish reporter lines to quantitatively measure the ATF6 branch of the endoplasmic reticulum stress pathway in development and disease.
Molecular Architecture of the Bardet-Biedl Syndrome Protein 2-7-9 Subcomplex
Authors: Ludlam W. Grant , Aoba Takuma , Cuéllar Jorge , Bueno-Carrasco M. Teresa , Makaju Aman , Moody James D. , Franklin Sarah , Valpuesta José M. , Willardson Barry M. 
Published: July 2019

SummaryBardet-Biedl syndrome (BBS) is a genetic disease caused by mutations that disrupt the function of the BBSome, an eight-subunit complex that plays an important role in transport of proteins in primary cilia. To better understand the molecular basis of the disease, we analyzed the structure of a BBSome subcomplex consisting of three homologous BBS proteins (BBS2, BBS7, and BBS9) by an integrative structural modeling approach using electron microscopy and chemical crosslinking coupled with mass spectrometry. The resulting molecular model revealed an overall structure that resembles a flattened triangle. Within the structure, BBS2 and BBS7 form a tight dimer based on a coiled-coil interaction, and BBS9 associates with the dimer via an interaction with the α-helical domain of BBS2. Interestingly, a BBS-linked mutation of BBS2 (R632P) is located in the α-helical domain at the interface between BBS2 and BBS9, and binding experiments showed that this mutation disrupted the interaction of BBS2 with BBS9. This finding suggests that BBSome assembly is disrupted by the R632P substitution, providing a molecular explanation for BBS in patients harboring this mutation.
First identification of trombiculid mites (Acari: Trombiculidae) on rodents captured on Chiloé Island, an endemic region of scrub typhus in southern Chile
Authors: Acosta-Jamett Gerardo , Beltrami Esperanza , de La Fuente María Carolina Silva , Martinez-Valdebenito Constanza , Weitzel Thomas , Abarca Katia 
Published: July 2019

AbstractBackgroundScrub typhus in an emerging vector-borne zoonosis, caused by Orientia spp. and transmitted by larvae of trombiculid mites, called chiggers. It mainly occurs within a certain region of the Asia-Pacific, called tsutsugamushi triangle, where rodents are known as the most relevant hosts for the trombiculid vector. The disease has recently been discovered on Chiloé Island in southern Chile. Still, the reservoir(s) and vector(s) of the scrub typhus outside Asia-Pacific are unknown. The aim of the present work was to study the prevalence of chiggers on different rodent species captured in sites identified as probably hot spots of scrub typhus on Chiloé Island in southern Chile.Methodology/Principal FindingsDuring austral summer 2018, rodents were live-trapped in six sites and examined for chigger infestation. During a total of 4,713 trap-nights, 244 rodents of seven species were captured: the most abundant was Abrothrix olivacea. All study sites were rural areas on Chiloé Island, previously identified as localities of probable human infection with scrub typhus. Chiggers were detected on all seven rodent species with a 55% prevalence rate. Chiggers showed low host specificity and varied according to site specific host abundance. We identified trombiculids of three genera. Colicus was the most abundant chigger (93%), prevalent in five of six sites, followed by Quadraseta (7%) and Paratrombicula (7%), which were in only one site. Infestation rates showed site specific differences, which were statistically different using a GLM model with binomial errors.Conclusions/SignificanceThis study firstly reports the presence of different rodent-associated chigger mites in a region with endemic scrub typhus in southern Chile. Colicus and two other genera of mites were found with high infestation rates in sites previously identified as hot spots of scrub typhus, suggesting their role as vectors and reservoirs of this emerging zoonosis in South America.Author SummaryScrub typhus is a chigger-transmitted zoonotic infection, which is endemic in the tsutsugamushi triangle in Asia-Pacific. Recently, a first focus of scrub typhus in South America has been confirmed on Chiloé Island in southern Chile. Still, the vectors of scrub typhus in this region remain unknown. We undertook a survey to study the prevalence of chiggers on different rodent species in areas identified as probable hot spots of scrub typhus on Chiloé Island. The study showed that 55% of rodents were infested by trombiculids. Three chigger genera were identified, of which Colicus was the most abundant. Chiggers showed low host specificity, but spatial differences. This first demonstration of rodent-associated chigger mites in hot spots of scrub typhus suggests their possible role as vectors of this infection in Chile.
Viral expression of a SERCA2a-activating PLB mutant improves calcium cycling and synchronicity in dilated cardiomyopathic hiPSC-CMs
Authors: Stroik Daniel R. , Ceholski Delaine K. , Mleczko Justyna , Thanel Paul F. , Bidwell Philip A. , Autry Joseph M. , Cornea Razvan L. , Thomas David D. 
Published: July 2019

AbstractThere is increasing momentum toward the development of gene therapy for heart failure (HF), cardiomyopathy, and other progressive cardiac diseases that correlate with impaired calcium (Ca2+) transport and reduced contractility. We have used FRET between fluorescently-tagged SERCA2a (the cardiac Ca2+ pump) and PLB (its ventricular peptide inhibitor) to test directly the effectiveness of loss-of-inhibition/gain-of-binding (LOI/GOB) PLB mutants (PLBM) that were engineered to compete with the binding of inhibitory wild type PLB (PLBWT). Our therapeutic strategy is to relieve PLBWT inhibition of SERCA2a by utilizing the reserve adrenergic capacity of PLB to enhance baseline cardiac contractility. Using a FRET assay, we determined that the combination of a LOI PLB mutation (L31A) and a GOB PLB mutation (I40A) results in a novel engineered LOI/GOB PLBM (L31A/I40A) that effectively competes with PLBWT binding to cardiac SERCA2a in HEK293-6E cells. We demonstrated that co-expression of L31A/I40A-PLBM enhances SERCA Ca-ATPase activity by increasing enzyme Ca2+ affinity (1/KCa) in PLBWT-inhibited HEK cell homogenates. For an initial assessment of PLBM physiological effectiveness, we used human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) from a healthy individual. In this system, we observed that adeno-associated virus 2 (rAAV2)-driven expression of L31A/I40A-PLBM enhances the amplitude of SR Ca2+ release and the rate of SR Ca2+ re-uptake. To assess therapeutic potential, we used an hiPSC-CM model of dilated cardiomyopathy (DCM) containing PLB mutation R14del, where we observed that rAAV2-driven expression of L31A/I40A-PLBM rescues arrhythmic Ca2+ transients and alleviates decreased Ca2+ transport. Based on these results, PLBM transgene expression is a promising gene therapy strategy for cardiomyopathies associated with impaired Ca2+ transport and decreased contractility.
Counter accounts of profit: outrage to action through “just” calculation
Authors: Himick Darlene , Ruff Kate 
Published: July 2019

Purpose
Profit is often moralized by activists, but scant research has carefully examined what profit is for these activists or how they use it to create a more just world. The purpose of this paper is to investigate how social movements use counter accounts of profit as tools of resistance.

Design/methodology/approach
A multiple case study design, informed by framing theory, is used to trace the framing of profit from activists’ counter accounts to actions they precipitated. Specifically, the study examines counter accounts of profit from the UK abolition movement, Médecines Sans Frontières access to essential medicines campaign and Brigitte Bardot Foundation’s opposition to the Canadian seal hunt, and how their framings of profit influenced change.

Findings
Activists reframe profit to create visibilities and bridges to the suffering of distant others. Reframing the calculation and boundary of profit is a strategy to elicit moral outrage, hope and ultimately a more just world. Through these reframings, activists in three different social movements were able to change the possibilities of who and what can be profitable, and how.

Social implications
The inherently incomplete nature of accounting frames give rise to accounting’s vulnerability to non-accountants to assert their views of a moral profit. Accounting therefore is both a means of control at a distance but also “emancipation at a distance.”

Originality/value
Scholars have asserted that accounting can be used for resistance, few studies have examined how. By examining how activists assert what profit is – and should be – the paper documents and theorizes profit as contested and highlights accounting’s emancipatory potential.
Non-equilibrium natural convection in a differentially-heated nanofluid cavity partially filled with a porous medium
Authors: Sheremet Mikhail A. , Pop Ioan , Baytas A. Cihat 
Published: July 2019

Purpose
This study aims to numerically analyze natural convection of alumina-water nanofluid in a differentially-heated square cavity partially filled with a heat-generating porous medium. A single-phase nanofluid model with experimental correlations for the nanofluid viscosity and thermal conductivity has been considered for the description of the nanoparticles transport effect in the present study. Local thermal non-equilibrium approach for the porous layer with the Brinkman-extended Darcy model has been used.

Design/methodology/approach
Dimensionless governing equations formulated using stream function, vorticity and temperature have been solved by the finite difference method. The effects of the Rayleigh number, Ostrogradsky number, Nield number and nanoparticles volume fraction on nanofluid flow, heat and mass transfer have been analyzed.

Findings
It has been revealed that the dimensionless heat transfer coefficient at the fluid/solid matrix interface can be a very good control parameter for the convective flow and heat transfer intensity. The present results are original and new for the study of non-equilibrium natural convection in a differentially-heated nanofluid cavity partially filled with a porous medium.

Originality/value
The results of this paper are new and original with many practical applications of nanofluids in the modern industry.
Motivation to teach
Authors: Leech Nancy L. , Viesca Kara Mitchell , Haug Carolyn A. 
Published: July 2019

Purpose
The purpose of this paper is to investigate higher education faculty’s motivation to teach and to validate the Factors Influencing Teaching Choice (FIT-Choice) survey with this population.

Design/methodology/approach
Confirmatory factor analysis and t-tests on data from 101 higher education faculty and data from K-12 teachers show that the two samples fit the model similarly.

Findings
Results show that the similarities between the two groups are important to note as it suggests both the value of the FIT-Choice instrument as a research tool in higher education as well as the similarities in motivating factors between higher education faculty and in-service K-12 teachers.

Originality/value
This is one of the first studies to use the FIT-Choice scale with university education faculty.
Impact of employees’ job control perceptions on their work-related responses in the hospitality industry
Authors: Lee Seonjeong (Ally) , Ravichandran Swathi 
Published: July 2019

PurposeThis study investigates the relationships among three types of employees’ perceived job control, employee commitment, employees’ well-being, and job performance.Design/methodology/approachThe proposed relationships were investigated based on a cross-section, online, self-administered survey.FindingsThe results confirmed the positive role of employees’ job control perceptions on work-related responses.Practical implicationsResults suggested hospitality managers implement practices to improve job control perceptions of employees to enhance their well-being and job performance.Originality/valueThis study was the first to investigate the roles of three types of job control perceptions on employees’ well-being and commitment, based on positive organizational behavior literature and control theory. This study was also the first attempt to explore three types of job control focusing on employees’ perspectives in the hospitality industry.
Recalibrating talent management for hospitality: a youth development perspective
Authors: Golubovskaya Maria , Solnet David , Robinson Richard N.S. 
Published: July 2019

Purpose
This paper aims to challenge existing assumptions in talent management (TM) research, showcasing a misalignment between commonly held assumptions and the characteristics of the youth-intensive hospitality sector workforce.

Design/methodology/approach
Following a review of the TM literature, Piirto’s educational talent pyramid is adopted to conceptualize a recalibration. Drawing on multidisciplinary literatures (i.e. adolescent development, youth employment, positive psychology), and adopting a (talent) developmental approach, a reframing of prevalent TM discourses is enunciated based on the logic that the hospitality workforce is predominantly in a developmental state.

Findings
TM discourses are misaligned with the workforce composition of the hospitality industry, which is dominated by young, often unexperienced, workers. The need for dramatically recalibrated TM structures and underlying assumptions, centred around a greater attention to the “development” of talent and a more employee-focused and inclusive approach, can facilitate greater alignment between TM and hospitality.

Research limitations/implications
This paper extends a body of work advocating for more inclusive TM and developmental postures. The contribution, via a hospitality industry context, has been to create linkages between talent- and youth-development discourses.

Practical implications
This paper outlines a number of implications, among which are a pathway forward for hospitality industry to rebuild its poor HRM image and conversion of “transient” hospitality jobs to career jobs (for youth).

Originality/value
This paper identifies youth as a distinct workforce entity and suggests that hospitality jobs represent a critical developmental context for young people, resulting in a series of critical implications for TM practice and theorizing.