bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Robotic Coordinated Control System for Bowl Food Packaging
Authors: Yequan Bao , Changsheng Ai , Dunyang Geng , Gangchang Ren , Hong Ju 
Published: October 2019

Abstract
The processing of the bowled food is relatively mechanical, repetitive and cumbersome, resulting in high work intensity, low efficiency, and high defect rate, and meets the working cycle:13.5s, positioning accuracy: 2mm.A PLC-based automatic feeding control system for food packaging line was designed. Including the conveyor line for bowl feeding, the numerical controlled manipulator and package process following control system for reclaiming and discharging functions, the system control scheme was proposed and 3D modeling construction was carried out. The design of hardware and software for feeding, reclaiming and discharging in the system is elaborated in detail. Finally, through the actual on-site commissioning and production operation, it is proved that the equipment has fully met the design requirements and greatly improved the production efficiency.
Numerical Analysis of Influence of Welding Sequence on Welding Deformation of Magnetic Separator Dielectric Box
Authors: Sun Xuan , Wang Zhiyong , Yingjun Li , Changsheng Ai 
Published: October 2019

Abstract
Aiming at the problem that the welding sequence has a great influence on the welding deformation of the dielectric box, this paper uses the nonlinear finite element method to numerically simulate the four welding sequences of the magnetic separator dielectric box, and analyzes the transverse deformation and longitudinal deformation. The welding sequence with the smallest amount of deformation was obtained, and the correctness of the simulation results was verified by experiments.
Septin 2/6/7 complexes tune microtubule plus-end growth and EB1 binding in a concentration- and filament-dependent manner
Authors: Nakos Konstantinos , Radler Megan R. , Spiliotis Elias T. 
Published: October 2019

Septins (SEPTs) are filamentous guanosine-5′-triphosphate (GTP)-binding proteins, which affect microtubule (MT)-dependent functions including membrane trafficking and cell division, but their precise role in MT dynamics is poorly understood. Here, in vitro reconstitution of MT dynamics with SEPT2/6/7, the minimal subunits of septin heteromers, shows that SEPT2/6/7 has a biphasic concentration-dependent effect on MT growth. Lower concentrations of SEPT2/6/7 enhance MT plus-end growth and elongation, while higher and intermediate concentrations inhibit and pause plus-end growth, respectively. We show that SEPT2/6/7 has a modest preference for GTP- over guanosine diphosphate (GDP)-bound MT lattice and competes with end-binding protein 1 (EB1) for binding to guanosine 5′- O-[γ-thio]triphosphate (GTPγS)-stabilized MTs, which mimic the EB1-preferred GDP-Pi state of polymerized tubulin. Strikingly, SEPT2/6/7 triggers EB1 dissociation from plus-end tips in cis by binding to the MT lattice and in trans when MT plus ends collide with SEPT2/6/7 filaments. At these intersections, SEPT2/6/7 filaments were more potent barriers than actin filaments in pausing MT growth and dissociating EB1 in vitro and in live cells. These data demonstrate that SEPT2/6/7 complexes and filaments can directly impact MT plus-end growth and the tracking of plus end–binding proteins and thereby may facilitate the capture of MT plus ends at intracellular sites of septin enrichment.[Media: see text]
Post-operative delirium associated with metabolic alterations following hemi-arthroplasty in older patients
Authors: Guo Yong , Li Yingchuan , Zhang Yinan , Fang Shudong , Xu Xiaotao , Zhao Aihua , Zhang Junfeng , Li Jia V , Ma Daqing , Jia Wei , Jiang Wei 
Published: October 2019

Abstract

Background
post-operative delirium (POD) is a common complication in older patients, though a possible link between metabolic changes and POD development has yet to be investigated.

Methods
older patients with hip fracture who underwent hemi-arthroplasty were recruited, and delirious states were assessed for 3 days after surgery using the confusion assessment method-Chinese revision. Simultaneously, fasting blood samples were collected on the morning of surgery and on the first post-operative day. Ultimately, 244 older patients who met the inclusion and exclusion criteria were assessed. Blood samples from 60 patients with POD and 60 matched controls were analysed using metabolomics platforms.

Results
sixty patients (24.6%) developed POD. Principal component analysis scores plot and cross-validated scores plots from orthogonal partial least squares-discriminant analysis were implemented to visualise the differences in metabolites between the two groups before and after surgery (P &lt; 0.05). Our data indicate that levels of ω3 and ω6 fatty acids were lower in the POD group than in the NPOD (non-POD) group both before and after surgery; tricarboxylic cycle intermediate levels were lower in the POD group than in the NPOD group, but glycolysis products were higher in the POD group than in the NPOD group after surgery. Furthermore, the branched-chain amino acid (BCAA)/aromatic amino acid ratio was lower in the POD group than in the NPOD group after surgery.

Conclusions
metabolic abnormalities, including deficiencies in ω3 and ω6 fatty acids, perturbations in tricarboxylic cycle and oxidative stress and metabolic imbalances in BCAA and AAA might contribute to POD development.
Distinguishing Causation from Correlation in the Use of Correlates of Protection to Evaluate and Develop Influenza Vaccines
Authors: Lim Wey Wen , Leung Nancy H L , Sullivan Sheena G , Tchetgen Tchetgen Eric J , Cowling Benjamin J 
Published: October 2019

Abstract
There is increasing attention to the need to identify new immune markers for the evaluation of existing and new influenza vaccines. Immune markers that could predict individual protection against infection and disease, commonly called correlates of protection (CoPs), play an important role in vaccine development and licensing. Here, we discuss the epidemiologic considerations when evaluating immune markers as potential CoPs for influenza vaccines and emphasize the distinction between correlation and causation. While an immune marker that correlates well with protection from infection can be used as a predictor of vaccine efficacy, it should be distinguished from an immune marker that plays a mechanistic role in conferring protection against a clinical endpoint—the latter might be a more reliable predictor of vaccine efficacy and a more appropriate target for rational vaccine design. To clearly distinguish mechanistic and nonmechanistic CoPs, we suggest using the term “correlates of protection” for nonmechanistic CoPs, and ‘‘mediators of protection’’ for mechanistic CoPs. Furthermore, because the interactions among and relative importance of correlates or mediators of protection can vary according to age or prior vaccine experience, the effect sizes and thresholds for protective effects for CoPs could also vary in different segments of the population.
The SAUR41 subfamily of SMALL AUXIN UP RNA genes is abscisic acid inducible to modulate cell expansion and salt tolerance in Arabidopsis thaliana seedlings
Authors: Qiu Ting , Qi Mengyuan , Ding Xiaohui , Zheng Yanyan , Zhou Tianjiao , Chen Yong , Han Ning , Zhu Muyuan , Bian Hongwu , Wang Junhui 
Published: October 2019

Abstract

Background and Aims
Most primary auxin response genes are classified into three families: AUX/IAA, GH3 and SAUR genes. Few studies have been conducted on Arabidopsis thaliana SAUR genes, possibly due to genetic redundancy among different subfamily members. Data mining on arabidopsis transcriptional profiles indicates that the SAUR41 subfamily members of SMALL AUXIN UP RNA genes are, strikingly, induced by an inhibitory phytohormone, abscisic acid (ABA). We aimed to reveal the physiological roles of arabidopsis SAUR41 subfamily genes containing SAUR40, SAUR41, SAUR71 and SAUR72.

Methods
Transcriptional responses of arabidopsis SAUR41 genes to phytohormones were determined by quantitative real-time PCR. Knock out of SAUR41 genes was carried out with the CRISPR/Cas9 (clustered regulatory interspaced short palindromic repeats/CRISPR-associated protein 9) genome editing technique. The saur41/40/71/72 quadruple mutants, SAUR41 overexpression lines and the wild type were subjected to ultrastructural observation, transcriptome analysis and physiological characterization.

Key Results
Transcription of arabidopsis SAUR41 subfamily genes is activated by ABA but not by gibberellic acids and brassinosteroids. Quadruple mutations in saur41/40/71/72 led to reduced cell expansion/elongation in cotyledons and hypocotyls, opposite to the overexpression of SAUR41; however, an irregular arrangement of cell size and shape was observed in both cases. The quadruple mutants had increased transcription of calcium homeostasis/signalling genes in seedling shoots, and the SAUR41 overexpression lines had decreased transcription of iron homeostasis genes in roots and increased ABA biosynthesis in shoots. Notably, both the quadruple mutants and the SAUR41 overexpression lines were hypersensitive to salt stress during seedling establishment, whereas specific expression of SAUR41 under the ABA-responsive RD29A (Responsive to Desiccation 29A) promoter in the quadruple mutants rescued the inhibitory effect of salt stress.

Conclusions
The SAUR41 subfamily genes of arabidopsis are ABA inducible to modulate cell expansion, ion homeostasis and salt tolerance. Our work may provide new candidate genes for improvement of plant abiotic stress tolerance.
Breaking Down the Pseudo-Pacification Process: Eight Critiques of Ultra-Realist Crime Causation Theory
Authors: Wood Mark A , Anderson Briony , Richards Imogen 
Published: October 2019

Abstract
This paper critically examines ultra-realist criminology’s two central crime causation theories: the breakdown of the pseudo-pacification process and special liberty. We identify a number of shortcomings in these theories pertaining to (1) their explanation of gender-related disparities in criminal offending; (2) their explanation of violence reduction through Freudian notions of drives, libidinal energy, and sublimation; and (3) their explication of crime as an expression of capitalist values. Fundamentally, we suggest that in treating political economy as the underlying source of all causative power in society, both theories engage in what Margaret Archer terms ‘downwards conflationism’. To this end, ultra-realism offers what we term a ‘direct expression theory of crime’, in which crime is a synecdoche and direct unmediated expression of political-economic conditions alone. Drawing on Margaret Archer’s realist social theory, we conclude by sketching out several potential principles of an ‘indirect expression theory’ that avoid the shortcomings of ultra-realism in explaining the complicated relationship between political economy and crime.
Trans Women’s Perceptions of Residential Aged Care in Australia
Authors: Waling Andrea , Lyons Anthony , Alba Beatrice , Minichiello Victor , Barrett Catherine , Hughes Mark , Fredriksen-Goldsen Karen , Edmonds Samantha 
Published: October 2019

AbstractMany older people in trans communities in Australia and elsewhere have experienced long histories of violence and discrimination in the health and social care sectors, making some of them fearful of interacting with contemporary health and social care providers. This study explored older trans women’s perceptions of these services. It involved a qualitative, thematic analysis of semi-structured, one-on-one audio-recorded interviews with ten trans women aged sixty years and older in Australia. Participants expressed a number of concerns about using residential facilities for older people in Australia, including potential for abuse and discrimination as a result of being trans, and not having access to appropriate treatments. Participants indicated a range of alternatives in using services, such as renovating the home, relocating to areas with greater access to trans-inclusive services and potential euthanasia. Participants perceived that service providers were not adequately trained for trans and gender diverse needs, and highlighted a number of ways aged care services could better support the trans and gender diverse community. The findings provide important information to assist health and social care professionals, including social workers, as well as residential care service providers, in supporting the health and well-being of older trans women.
Phylogeography of Excoecaria acerifolia (Euphorbiaceae) suggests combined effects of historical drainage reorganization events and climatic changes on riparian plants in the Sino–Himalayan region
Authors: Wang Zhi-Wei , Zhang Ti-Cao , Luo Dong , Sun Wen-Guang , Sun Hang 
Published: October 2019

AbstractIt has been hypothesized that geological and climatic changes in the Sino–Himalayan region played a significant role in evolutionary history. In this study, we tested this hypothesis by investigating the phylogeography of Excoecaria acerifolia (Euphorbiaceae), a riparian plant species that is widely distributed in the hot/warm-dry river valleys of the Sino–Himalayan region. Spatial analysis of molecular variance, a median-joining network and a Bayesian phylogenetic tree based on plastid DNA, all suggested three major lineages corresponding to the Jialing-Min-Dadu (JMD lineage), Jinsha-Yalong-Salween (JY lineage) and Yarlung Tsangpo-Mekong-Red-Nanpan (YMRN lineage) drainage basins. This was also generally supported by the results based on nuclear DNA. The divergence times of these three major lineages based on both datasets fell in the early Pleistocene, coinciding with the period of drainage reorganization events in the Sino–Himalayan region. The diversification times in the lineages were, however, dated back to the mid Pleistocene, corresponding to the Naynayxungla glaciation (0.72–0.50 Mya) and the penultimate glaciation (0.30–0.13 Mya), which were the most and second most severe glaciations in this region, respectively. Furthermore, mismatch analyses, neutrality test and ecological niche modelling suggest long-term demographic stability of the JY and JMD populations, with expansion only in the YMRN populations during the period(s) from the late penultimate glaciation (138.12 Kya) to the Last Interglacial (95.79 Kya), probably because of less extensive glaciations since the late Pleistocene and the gradually warming interglacial stage. Our study provides one of the few pieces of evidence indicating that combined historical drainage reorganization and climatic change since the Pleistocene might also have acted as important factors in the evolutionary history of riparian plants in the region.
The structural connectivity of discrete networks underlies impulsivity and gambling in Parkinson’s disease
Authors: Mosley Philip E , Paliwal Saee , Robinson Katherine , Coyne Terry , Silburn Peter , Tittgemeyer Marc , Stephan Klaas E , Breakspear Michael , Perry Alistair 
Published: October 2019

See O’Callaghan (doi:10.1093/brain/awz349) for a scientific commentary on this article.
Mosley et al. examine impulsivity and naturalistic gambling behaviours in patients with Parkinson’s disease. They link within-patient differences to the structural connectivity of networks subserving reward evaluation and response inhibition, and reveal pivotal roles for the ventral striatum and subthalamic nucleus within these networks.
Phospho-valproic acid (MDC-1112) reduces pancreatic cancer growth in patient-derived tumor xenografts and KPC mice: enhanced efficacy when combined with gemcitabine
Authors: Luo Dingyuan , Digiovanni Matthew G , Wei Ran , Lacomb Joseph F , Williams Jennie L , Rigas Basil , Mackenzie Gerardo G 
Published: October 2019

Abstract
New chemotherapeutic agents are needed for pancreatic cancer (PC). We have previously shown that phospho-valproic acid (MDC-1112) is effective in cell-line xenografts of PC. Here, we explored whether MDC-1112 is effective in additional clinically relevant animal models of PC and whether MDC-1112 enhances the anticancer effect of clinically used chemotherapeutic agents. MDC-1112 alone strongly reduced patient-derived pancreatic tumor xenograft growth, and extended survival of LSL-KrasG12D/+; LSL-Trp53R172H/+; Pdx1-Cre (KPC) mice. In both models, MDC-1112 inhibited STAT3 activation and its downstream signals, including Bcl-xL and cyclin D1. In human PC cell lines, P-V enhanced the growth inhibitory effect of gemcitabine (GEM), Abraxane and 5-FU, but not that of irinotecan. Normal human pancreatic epithelial cells were more resistant to the cytotoxic effects of MDC-1112/GEM combination. Furthermore, MDC-1112 enhanced GEM’s effect on colony formation, apoptosis, cell migration, and cell invasion. In vivo, MDC-1112 and GEM, given alone, reduced patient-derived pancreatic tumor xenograft growth by 58% and 87%, respectively; whereas MDC-1112/GEM combination reduced tumor growth by 94%, inducing tumor stasis. In conclusion, MDC-1112 should be further explored as a potential agent to be used in combination with GEM for treating PC.
Ketamine Restores Thalamic-Prefrontal Cortex Functional Connectivity in a Mouse Model of Neurodevelopmental Disorder-Associated 2p16.3 Deletion
Authors: B Hughes Rebecca , Whittingham-Dowd Jayde , Simmons Rachel E , Clapcote Steven J , Broughton Susan J , Dawson Neil 
Published: October 2019

Abstract2p16.3 deletions, involving heterozygous NEUREXIN1 (NRXN1) deletion, dramatically increase the risk of developing neurodevelopmental disorders, including autism and schizophrenia. We have little understanding of how NRXN1 heterozygosity increases the risk of developing these disorders, particularly in terms of the impact on brain and neurotransmitter system function and brain network connectivity. Thus, here we characterize cerebral metabolism and functional brain network connectivity in Nrxn1α heterozygous mice (Nrxn1α+/− mice), and assess the impact of ketamine and dextro-amphetamine on cerebral metabolism in these animals. We show that heterozygous Nrxn1α deletion alters cerebral metabolism in neural systems implicated in autism and schizophrenia including the thalamus, mesolimbic system, and select cortical regions. Nrxn1α heterozygosity also reduces the efficiency of functional brain networks, through lost thalamic “rich club” and prefrontal cortex (PFC) hub connectivity and through reduced thalamic-PFC and thalamic “rich club” regional interconnectivity. Subanesthetic ketamine administration normalizes the thalamic hypermetabolism and partially normalizes thalamic disconnectivity present in Nrxn1α+/− mice, while cerebral metabolic responses to dextro-amphetamine are unaltered. The data provide new insight into the systems-level impact of heterozygous Nrxn1α deletion and how this increases the risk of developing neurodevelopmental disorders. The data also suggest that the thalamic dysfunction induced by heterozygous Nrxn1α deletion may be NMDA receptor-dependent.
Multiple Morphological Factors Underlie Experience-Dependent Cross-Modal Plasticity in the Developing Sensory Cortices
Authors: Wang Miao , Yu Zixian , Li Guangying , Yu Xiang 
Published: October 2019

Abstract
Sensory experience regulates the structural and functional wiring of sensory cortices. In previous work, we showed that whisker deprivation (WD) from birth not only reduced excitatory synaptic transmission of layer (L) 2/3 pyramidal neurons of the correspondent barrel cortex in mice, but also cross-modally reduced synaptic transmission of L2/3 pyramidal neurons in other sensory cortices. Here, we used in utero electroporation, in combination with optical clearing, to examine the main morphological components regulating neural circuit wiring, namely presynaptic bouton density, spine density, as well as dendrite and axon arbor lengths. We found that WD from P0 to P14 reduced presynaptic bouton density in both L4 and L2/3 inputs to L2/3 pyramidal neurons, as well as spine density across the dendritic tree of L2/3 pyramidal neurons, in the barrel field of the primary somatosensory cortex. The cross-modal effects in the primary auditory cortex were manifested mostly as reduced dendrite and axon arbor size, as well as reduced bouton density of L2/3 inputs. Increasing sensory experience by rearing mice in an enriched environment rescued the effects of WD. Together, these results demonstrate that multiple morphological factors contribute to experience-dependent structural plasticity during early wiring of the sensory cortices.
Decreases in Antimicrobial Use Associated With Multihospital Implementation of Electronic Antimicrobial Stewardship Tools
Authors: Graber Christopher J , Jones Makoto M , Goetz Matthew Bidwell , Madaras-Kelly Karl , Zhang Yue , Butler Jorie M , Weir Charlene , Chou Ann F , Youn Sarah Y , Samore Matthew H , Glassman Peter A 
Published: October 2019

AbstractBackgroundAntimicrobial stewards may benefit from comparative data to inform interventions that promote optimal inpatient antimicrobial use.MethodsAntimicrobial stewards from 8 geographically dispersed Veterans Affairs (VA) inpatient facilities participated in the development of antimicrobial use visualization tools that allowed for comparison to facilities of similar complexity. The visualization tools consisted of an interactive web-based antimicrobial dashboard and, later, a standardized antimicrobial usage report updated at user-selected intervals. Stewards participated in monthly learning collaboratives. The percent change in average monthly antimicrobial use (all antimicrobial agents, anti-methicillin-resistant Staphylococcus aureus [anti-MRSA] agents, and antipseudomonal agents) was analyzed using a pre–post (January 2014–January 2016 vs July 2016–January 2018) design with segmented regression and external comparison with uninvolved control facilities (n = 118).ResultsIntervention sites demonstrated a 2.1% decrease (95% confidence interval [CI], −5.7% to 1.6%) in total antimicrobial use pre–post intervention vs a 2.5% increase (95% CI, 0.8% to 4.1%) in nonintervention sites (absolute difference, 4.6%; P = .025). Anti-MRSA antimicrobial use decreased 11.3% (95% CI, −16.0% to −6.3%) at intervention sites vs a 6.6% decrease (95% CI, −9.1% to −3.9%) at nonintervention sites (absolute difference, 4.7%; P = .092). Antipseudomonal antimicrobial use decreased 3.4% (95% CI, −8.2% to 1.7%) at intervention sites vs a 3.6% increase (95% CI, 0.8% to 6.5%) at nonintervention sites (absolute difference, 7.0%; P = .018).ConclusionsComparative data visualization tool use by stewards at 8 VA facilities was associated with significant reductions in overall antimicrobial and antipseudomonal use relative to uninvolved facilities.