bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Epistasis among QTLs on Heading Date based on Single Segment Substitution Lines in Rice
Authors: Yang Zifeng , Jin Lingling , Zhu Haitao , Wang Shaokui , Zhang Guiquan , Liu Guifu 
Published: February 2018

AbstractHeading date directly determines the planting districts and seasons, and thus plays an important role for producing and introducing of varieties. Limited to the materials and methodologies, analysis of epistasis still presents an obvious challenge. This thesis estimated effectively four types of epistatic components among dual QTLs on heading date based on eight single segment substitution lines (SSSLs) in rice. The results confirmed that they carried truly with heading date QTLs. Eleven pairs of QTLs were with 50.0% of significant epistatic effects, of which additive-additive, additive-dominance or dominance-additive, and dominance-dominance interaction components occupied 40.9%, 50.0% and 59.1%, respectively. One QTL always interacted with multiple QTLs in various components. Several characteristics of epistasis on heading date were found that 1) different epistatic components had almost consistent directions; 2) dominance-dominance epistasis was perhaps most important in the four epistatic components; 3) epistasis was mostly positive, delaying rice heading; and 4) all epistatic components were seasonal sensitive. Two flowering pathways were further confirmed via a network constructed among these QTLs. These results have further confirmed the prevalence of epistatic interactions, deepened the understanding of genetic and flowering mechanism, and excavated several advantageous genes on heading date in rice.
An in silico argument for mitochondrial microRNA as a determinant of primary non function in liver transplantation
Authors: Khorsandi Shirin Elizabeth , Salehi Siamak , Cortes Miriam , Vilca-Melendez Hector , Menon Krishna , Srinivasan Parthi , Prachalias Andreas , Jassem Wayel , Heaton Nigel 
Published: February 2018

AbstractMitochondria have their own genomic, transcriptomic and proteomic machinery but are unable to be autonomous, needing both nuclear and mitochondrial genomes. The aim of this work was to use computational biology to explore the involvement of Mitochondrial microRNAs (MitomiRs) and their interactions with the mitochondrial proteome in a clinical model of primary non function (PNF) of the donor after cardiac death (DCD) liver. Archival array data on the differential expression of miRNA in DCD PNF was re-analyzed using a number of publically available computational algorithms. 10 MitomiRs were identified of importance in DCD PNF, 7 with predicted interaction of their seed sequence with the mitochondrial transcriptome that included both coding, and non coding areas of the hypervariability region 1 (HVR1) and control region. Considering miRNA regulation of the nuclear encoded mitochondrial proteome, 7 hypothetical small proteins were identified with homolog function that ranged from co-factor for formation of ATP Synthase, REDOX balance and an importin/exportin protein. In silico, unconventional seed interactions, both non canonical and alternative seed sites, appear to be of greater importance in MitomiR regulation of the mitochondrial genome. Additionally, a number of novel small proteins of relevance in transplantation have been identified which need further characterization.
Neutralization of IL-15 abrogates experimental immune-mediated cholangitis in diet-induced obese mice
Authors: Reyes José L. , Vannan Danielle T. , Vo Tina , Gulamhusein Aliya , Beck Paul L. , Reimer Raylene A. , Eksteen Bertus 
Published: February 2018

AbstractObesity is a global epidemic affecting chronic inflammatory diseases. Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease that can occur as an extraintestinal manifestation of inflammatory bowel disease (IBD). Previously we reported that patients with PSC who are obese have a higher risk of advanced liver disease. Currently it is unknown how obesity accelerates or worsens PSC. We evaluated the progression of PSC in an antigen-driven cholangitis mouse model of diet-induced obesity. Obesity was induced in our murine model of immune-mediated cholangitis (OVAbil). OVAbil mice were fed standard chow or high-fat/sucrose diet for twelve weeks followed by induction of biliary inflammation by OVA-specific T cell transfer. Histopathological damage in portal tracts was scored and serum collected. Neutralizing antibodies against IL-15 were administered daily until study termination. Obese mice developed exacerbated liver inflammation and damage. Immune cell phenotyping in liver revealed greater numbers of neutrophils and CD8+ T cells in obese mice. Higher levels of cytokines and chemokines were found in obese mice with cholangitis. Immuno-neutralizing antibodies against IL-15 greatly attenuated cholangitis in obese mice. Obesity exacerbated experimental PSC in part by overproduction of IL-15. Timely targeting of IL-15 may slow the progression of PSC.
Prime and Boost Vaccination Elicit a Distinct Innate Myeloid Cell Immune Response
Authors: Palgen Jean-Louis , Tchitchek Nicolas , Elhmouzi-Younes Jamila , Delandre Simon , Namet Inana , Rosenbaum Pierre , Dereuddre-Bosquet Nathalie , Martinon Frédéric , Cosma Antonio , Lévy Yves , Le Grand Roger , Beignon Anne-Sophie 
Published: February 2018

AbstractUnderstanding the innate immune response to vaccination is critical in vaccine design. Here, we studied blood innate myeloid cells after first and second immunization of cynomolgus macaques with the modified vaccinia virus Ankara. The inflammation at the injection site was moderate and resolved faster after the boost. The blood concentration of inflammation markers increased after both injections but was lower after the boost. The numbers of neutrophils, monocytes, and dendritic cells were transiently affected by vaccination, but without any major difference between prime and boost. However, phenotyping deeper those cells with mass cytometry unveiled their high phenotypic diversity with subsets responding differently after each injection, some enriched only after the primary injection and others only after the boost. Actually, the composition in subphenotype already differed just before the boost as compared to just before the prime. Multivariate analysis identified the key features that contributed to these differences. Cell subpopulations best characterizing the post-boost response were more activated, with a stronger expression of markers involved in phagocytosis, antigen presentation, costimulation, chemotaxis, and inflammation. This study revisits innate immunity by demonstrating that, like adaptive immunity, innate myeloid responses differ after one or two immunizations.
Threshold driven contagion on weighted networks
Authors: Unicomb Samuel , Iñiguez Gerardo , Karsai Márton 
Published: February 2018

AbstractWeighted networks capture the structure of complex systems where interaction strength is meaningful. This information is essential to a large number of processes, such as threshold dynamics, where link weights reflect the amount of influence that neighbours have in determining a node's behaviour. Despite describing numerous cascading phenomena, such as neural firing or social contagion, the modelling of threshold dynamics on weighted networks has been largely overlooked. We fill this gap by studying a dynamical threshold model over synthetic and real weighted networks with numerical and analytical tools. We show that the time of cascade emergence depends non-monotonously on weight heterogeneities, which accelerate or decelerate the dynamics, and lead to non-trivial parameter spaces for various networks and weight distributions. Our methodology applies to arbitrary binary state processes and link properties, and may prove instrumental in understanding the role of edge heterogeneities in various natural and social phenomena.
Nuclear targeting of the betanodavirus B1 protein via two arginine-rich domains induces G1/S cell cycle arrest mediated by upregulation of p53/p21
Authors: Su Yu-Chin , Reshi Latif , Chen Lei-Jia , Li Wei-Han , Chiu Hsuan-Wen , Hong Jiann-Ruey 
Published: February 2018

AbstractThe molecular functions of betanodavirus non-structural protein B and its role in host cell survival remain unclear. In the present study, we examined the roles of specific nuclear targeting domains in B1 localization as well as the effect of B1 nuclear localization on the cell cycle and host cell survival. The B1 protein of the Red spotted grouper nervous necrosis virus (RGNNV) was detected in GF-1 grouper cells as early as 24 hours post-infection (hpi). Using an EYFP-B1 fusion construct, we observed nuclear localization of the B1 protein (up to 99%) in GF-1 cells at 48 hpi. The nuclear localization of B1 was mediated by two arginine-rich nuclear targeting domains (B domain: 46RRSRR51; C domain: 63RDKRPRR70) and domain C was more important than domain B in this process. B1 nuclear localization correlated with upregulation of p53 and p21(wef1/cip1); downregulation of Cyclin D1, CDK4 and Mdm2; and G1/S cell cycle arrest in GF-1 cells. In conclusion, nuclear targeting of the RGNNV B1 protein via two targeting domains causes cell cycle arrest by up-regulating p53/p21 and down-regulating Mdm2, thereby regulating host cell survival.
Probing Conformal Invariant of Non-unitary Two-Dimensional Systems by Central Spin Decoherence
Authors: Wei Bo-Bo 
Published: February 2018

AbstractUniversality classes of non-unitary critical theories in two-dimensions are characterized by the central charge. However, experimental determination of the central charge of a non-unitary critical theory has not been done before because of the intrinsic difficulty that complex parameters usually occur in non-unitary theory, which is not physical. Here we propose to extract the effective central charge of the non-unitary critical point of a two-dimensional lattice model from the quantum coherence measurement of a probe spin which is coupled to the lattice model. A recent discovery shows that quantum coherence of a probe spin which is coupled to a bath is proportional to the partition function of the bath with a complex parameter. Thus the effective central charge of a non-unitary conformal field theory may be extracted from quantum coherence measurement of a probe spin which is coupled to a bath. We have applied the method to the Yang-Lee edge singularity of the two-dimensional Ising model and extracted the effective central charge of the Yang-Lee edge singularity with good precision and tested other predictions of non-unitary conformal field theory. This work paves the way for the first experimental observation of the effective central charge of non-unitary conformal field theory.
Incidence and clinical characteristics of ciguatera fish poisoning in Guadeloupe (French West Indies) between 2013 and 2016: a retrospective cases-series
Authors: Boucaud-Maitre Denis , Vernoux Jean-Paul , Pelczar Stéphane , Daudens-Vaysse Elise , Aubert Lyderic , Boa Sylvie , Ferracci Serge , Garnier Robert 
Published: February 2018

AbstractThis retrospective case study analysed the incidence and symptoms of ciguatera fish poisoning (ciguatera) in Guadeloupe (French West Indies) between 2013 and 2016. Cases attending the emergency departments of the two public hospitals and the reports received by the regional health authority in charge of monitoring (ARS) were compiled. Two hundred and thirty-four cases of poisoning were observed, with a mean annual incidence of 1.47/10,000 (95% CI): 1.29–1.66), i.e 5 times higher than the previously reported incidence (1996–2006). The main species described as being responsible for poisoning were fish from the Carangidae family (n = 47) (jack), followed by fish from the Lutjanidae family (n = 27) (snapper), Serranidae family (n = 15) (grouper), Sphyraenidae family (n = 12) (barracuda), and Mullidae family (n = 12) (goatfish). One case of lionfish ciguatera was observed. 93.9% of patients experienced gastrointestinal symptoms, 76.0% presented neurological signs (mainly paresthesia, dysesthesia and pruritus) and 40.3% presented cardiovascular symptoms (bradycardia and/or hypotension). A high frequency (61.4%) of hypothermia (body temperature <36.5 °C) was observed. This study reports for the first time the relatively high frequency of cardiac symptoms and low body temperature. The monitoring of ciguatera poisoning throughout the Caribbean region must be improved, notably after reef disturbance due to Irma and Maria major cyclones.
Assessment of interaction between maternal polycyclic aromatic hydrocarbons exposure and genetic polymorphisms on the risk of congenital heart diseases
Authors: Li Nana , Mu Yi , Liu Zhen , Deng Ying , Guo Yixiong , Zhang Xuejuan , Li Xiaohong , Yu Ping , Wang Yanping , Zhu Jun 
Published: February 2018

AbstractThe major causes of congenital heart diseases (CHDs) are the interactions of genetic and environmental factors. We conducted a case–control study in 357 mothers of CHDs fetuses and 270 control mothers to investigate the association of maternal PAHs exposure, AHR, CYP1A1, CYP1A2, CYP1B1 and CYP2E polymorphisms, the interaction between PAHs exposure and genetic variants with the risk of CHDs. The higher level PAHs exposure was associated with the risk of CHDs (aOR = 2.029, 95% CI: 1.266, 3.251) or subtypes. The haplotypes of AHR or CYP1A2 were associated with the risk of CHDs: AHR: C-G-A-C: aOR = 0.765; T-A-G-A: aOR = 1.33; CYP1A2: A-T:aOR = 1.75; C-C: aOR = 0.706. When exposed to higher level PAHs, the risk of CHDs among the mothers carrying rs2158041 “C/T or T/T” genotype or rs7811989 “G/A or A/A” genotype in AHR was 1.724 (χ2 = 7.209, P = 0.007) or 1.735 (χ2 = 7.364, P = 0.007) times greater than the aOR in the mothers carrying wild genotype. The multiplicative-scale interactions between PAHs exposure and polymorphisms of CYP1A2 rs4646425 (P = 0.03) or CYP2E1 rs915908 (P = 0.0238) on the risk of CHDs were observed. Our study suggests that maternal AHR polymorphisms may modify the association of PAHs exposure with CHDs, CYP1A2 or CYP2E1 polymorphisms significantly interact with PAHs exposure on CHDs.
A Multifunctional Nanocage-based MOF with Tri- and Tetranuclear Zinc Cluster Secondary Building Units
Authors: Zhou Zhongyuan , Xing Xiushuang , Tian Chongbin , Wei Wei , Li Dejing , Hu Falu , Du Shaowu 
Published: February 2018

AbstractA new Zn-cluster based MOF, [Zn21(BTC)11(μ3-OH)3(μ4-O)3(H2O)18]·21EtOH (1) (H3BTC = 1,3,5-benzenetricarboxylic acid), with two different types of cluster nodes has been successfully synthesized from Zn2+ and H3BTC under the solvothermal conditions. Single crystal X-ray diffraction studies reveal that 1 is a 3D trinodal (3,5,6)-c framework which features a large octahedral cage organized by nine Zn3O and nine Zn4O clusters SBUs and twenty-four triangular BTC3− linkers. The Eu3+/Tb3+-incorporated derivative of 1 with 0.251% Eu3+ and 0.269% Tb3+ exhibits tunable luminescence from yellow to white and then to blue-green by changing the excitation wavelength from 308 to 315 nm. Metal ion exchange with Cu2+ affords isomorphous Cu-based MOF with enhanced N2 and CO2 adsorption capacity. In addition, 1 can act as a selective luminescent sensor for Cu2+ and Al3+ ions.
Neural activity induced by visual food stimuli presented out of awareness: a preliminary magnetoencephalography study
Authors: Takada Katsuko , Ishii Akira , Matsuo Takashi , Nakamura Chika , Uji Masato , Yoshikawa Takahiro 
Published: February 2018

AbstractObesity is a major public health problem in modern society. Appetitive behavior has been proposed to be partially driven by unconscious decision-making processes and thus, targeting the unconscious cognitive processes related to eating behavior is essential to develop strategies for overweight individuals and obese patients. Here, we presented food pictures below the threshold of awareness to healthy male volunteers and examined neural activity related to appetitive behavior using magnetoencephalography. We found that, among participants who did not recognize food pictures during the experiment, an index of heart rate variability assessed by electrocardiography (low-frequency component power/high-frequency component power ratio, LF/HF) just after picture presentation was increased compared with that just before presentation, and the increase in LF/HF was negatively associated with the score for cognitive restraint of food intake. In addition, increased LF/HF was negatively associated with increased alpha band power in Brodmann area (BA) 47 caused by food pictures presented below the threshold of awareness, and level of cognitive restraint was positively associated with increased alpha band power in BA13. Our findings may provide valuable clues to the development of methods assessing unconscious regulation of appetite and offer avenues for further study of the neural mechanisms related to eating behavior.
Substructure-activity relationship studies on antibody recognition for phenylurea compounds using competitive immunoassay and computational chemistry
Authors: Zhang Fuyuan , Liu Bing , Liu Guozhen , Zhang Yan , Wang Junping , Wang Shuo 
Published: February 2018

AbstractBased on the structural features of fluometuron, an immunizing hapten was synthesized and conjugated to bovine serum albumin as an immunogen to prepare a polyclonal antibody. However, the resultant antibody indicated cross-reactivity with 6 structurally similar phenylurea herbicides, with binding activities (expressed by IC50 values) ranging from 1.67 µg/L to 42.71 µg/L. All 6 phenylurea herbicides contain a common moiety and three different substitutes. To understand how these three different chemical groups affect the antibody-phenylurea recognition activity, quantum chemistry, using density function theory (DFT) at the B3LYP/6-311++ G(d,p) level of theory, was employed to optimize all phenylurea structures, followed by determination of the 3D conformations of these molecules, pharmacophore analysis, and molecular electrostatic potential (ESP) analysis. The molecular modeling results confirmed that the geometry configuration, pharmacophore features and electron distribution in the substituents were related to the antibody binding activity. Spearman correlation analysis further elucidated that the geometrical and electrostatic properties on the van der Waals (vdW) surface of the substituents played a critical role in the antibody-phenylurea recognition process.
Phaeophleospora vochysiae Savi & Glienke sp. nov. Isolated from Vochysia divergens Found in the Pantanal, Brazil, Produces Bioactive Secondary Metabolites
Authors: Savi Daiani C. , Shaaban Khaled A. , Gos Francielly Maria Wilke Ramos , Ponomareva Larissa V. , Thorson Jon S. , Glienke Chirlei , Rohr Jürgen 
Published: February 2018

AbstractMicroorganisms associated with plants are highly diverse and can produce a large number of secondary metabolites, with antimicrobial, anti-parasitic and cytotoxic activities. We are particularly interested in exploring endophytes from medicinal plants found in the Pantanal, a unique and widely unexplored wetland in Brazil. In a bio-prospecting study, strains LGMF1213 and LGMF1215 were isolated as endophytes from Vochysia divergens, and by morphological and molecular phylogenetic analyses were characterized as Phaeophleospora vochysiae sp. nov. The chemical assessment of this species reveals three major compounds with high biological activity, cercoscosporin (1), isocercosporin (2) and the new compound 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone (3). Besides the isolation of P. vochysiae as endophyte, the production of cercosporin compounds suggest that under specific conditions this species causes leaf spots, and may turn into a pathogen, since leaf spots are commonly caused by species of Cercospora that produce related compounds. In addition, the new compound 3-(sec-butyl)-6-ethyl-4,5-dihydroxy-2-methoxy-6-methylcyclohex-2-enone showed considerable antimicrobial activity and low cytotoxicity, which needs further exploration.
Familial Risks Between Urolithiasis and Cancer
Authors: Hemminki Kari , Hemminki Otto , Försti Asta , Sundquist Jan , Sundquist Kristina , Li Xinjun 
Published: February 2018

AbstractUrolithiasis (UL, urinary tract stone disease) has been reported to increase subsequent cancers in the urinary tract. Recently, we showed data that surveillance bias may be an important confounder in the reported associations. In the present approach we want to address the question of possible cancer risk posed by UL mechanistically. Both UL and cancer have strong genetic components and we hypothesize that familial association between UL and cancer may be plausible. We thus assess familial risks between UL and cancer, hoping to find an explanation why UL may pose a risk of cancer. UL patients were identified from hospital inpatient and outpatient records and they were organized in families based on the Multigeneration Register into which also national cancer data were linked. Standardized incidence ratios were calculated for cancer in the offspring generation when parents were diagnosed with UL, and conversely for UL when parents were diagnosed with cancer. Familial risks between UL and cancer were generally small and inconsistent providing no convincing support of genetic sharing between UL and cancer. However, bladder UL was associated weakly with prostate cancer, and ureter and bladder UL were associated with salivary gland cancer. Potential mechanisms for these findings are proposed.
Kinesthetic stimulation for obstructive sleep apnea syndrome: An “on-off” proof of concept trial
Authors: Hernández Alfredo I. , Pérez Diego , Feuerstein Delphine , Loiodice Corinne , Graindorge Laurence , Guerrero Gustavo , Limousin Nadège , Gagnadoux Frédéric , Dauvilliers Yves , Tamisier Renaud , Prigent Arnaud , Mabo Philippe , Amblard Amel , Senhadji Lotfi , Pépin Jean-Louis 
Published: February 2018

AbstractObstructive sleep apnea (OSA) occurs when the upper airway narrows or collapses due to the loss of upper airway muscle activation at sleep onset. This study investigated the effectiveness of triggered kinesthetic stimulation in patients with OSA. This proof-of-concept, open-label, multicenter prospective study was conducted on 24 patients with severe OSA. During a one night evaluation, kinesthetic stimulation was intermittently delivered in 30 minute periods. The duration of apneas and hypopneas during Stim
on
and Stim
off
periods were compared. Five hospital-based university centers in France participated. Sleep studies were evaluated by a single scorer at a core laboratory (CHU Grenoble). Results show that during the Stim
on
phases, statistically significant decreases in durations of apneas and hypopneas were observed in 56% and 46% of patients, respectively. Overall, 75% of patients showed an improvement in apneas or hypopneas durations. The mean reduction in durations for patients with a significant decrease was 4.86 seconds for apneas and 6.00 seconds for hypopneas. This proof of concept study is the first to identify kinesthetic stimulation as a potentially effective therapy for OSA. These data justify evaluation in a controlled study.