bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogrouping, Virulence Profile and Antibiogram of Escherichia coli Strains Associated with Canine Pyometra
Authors: Archana T.S. , Sankar Surya , Mani Binu K. , Harshan Hiron M. , Vidya P. , Anand Amrutha , Abraham Bincy K. 
Published: December 2024

Background: Multi-drug resistant bacterial strains with biofilm-forming ability are one of the significant reasons contributing to the failure of medical management and recurrence of canine pyometra. The predominant bacterial agent implicated in canine pyometra is Escherichia coli, the different strains of which can be grouped into distinct phylogroups with the same ecological niches, characteristics and tendency to cause disease. The identification of a phylogroup of an unknown strain can facilitate the control, prevention and treatment of infections. Methods: The isolates of E.coli obtained from 25 clinical cases of pyometra were identified based on the cultural, morphological and biochemical characteristics. The antibiogram of the isolates were done employing Kirby-Bauer disk diffusion method. Biofilm forming ability of theisolates were detected by tissue culture plate method and the presence of virulence genes among them were detected using a multiplex PCR. The phylogrouping of E. coli was done employing the novel quadruplex PCR method. Result: In the antibiogram, most isolates were found sensitive to Amoxycillin-clavulanate (90%), Gentamicin (80%),Tetracycline (50%) and Amikacin (50%), while 100% resistance were shown towards ceftazidime-clavulanate, enrofloxacin,ceftriaxone-tazobactam, ciprofloxacin and cefotaxime-clavulanate. Among the E. coli isolates, strong and moderate biofilm producers showed resistance to a wide range of antibiotics compared to non-biofilm producers. Around 80% of the isolates belonged to the phylogroup B2 and all of these had the presence of fimH, sfa, and csgA genes. Among the isolates belonged to the phylogroup B2, 87.5% of the isolates had pap gene while none of them possessed afa gene.
A Method of Applying Mixtures to Optimize Grounding during Installation of Vertical Composite Grounding Devices
Authors: Pavlovich I. A. , Baraishuk S. M. , Murodov M. , Nabiev M. 
Published: December 2024

The article considers the method of using mixtures to optimize the electrophysical parameters of grounding devices in conjunction with vertical composite grounding conductors. It has been found that fluctuations in soil resistivity caused by changes in weather and climatic conditions can lead to instability of the ground loop resistance. The study shown that without appropriate measures, the resistance of the loop as a result of seasonal changes in soil properties may exceed acceptable values. This is fraught with deviations in the resistance to current spreading of grounding devices beyond the limits of acceptable parameters. To compensate for these fluctuations, a method is proposed to reduce the seasonality factor. Reducing seasonality plays an important role in ensuring the safety of service personnel and farm animals by maintaining the resistance of the grounding device within the limits of regulatory values. The authors discuss methods for artificially reducing the resistance of the ground loop, including increasing its size and using deep ground electrodes. The results of vertical electrode probing of the soil at the sites of grounding conductors are presented, the effect of humidity on the resistivity of the soil is shown, the influence of soil layering and the presence of moisture-saturated soil layers is considered. A method is proposed that allows the mixture to be introduced together with a vertical composite grounding device, the design of the coupling, tip and auxiliary device, experimental studies of the proposed designs are carried out and the results of measuring the current spreading resistance of such a grounding device with both standard and proposed couplings are presented. A comparison was made with a grounding device without the use of mixtures. The measurement results demonstrate that with an increase in the length of the grounding device, its diameter and the volume of the injected mixture, the resistance decreases. It is shown that the proposed solution makes it possible to reduce seasonality by 1.64–2.1 times, depending on the couplings used, and to obtain a grounding conductor with an equivalent diameter dozens of times larger than the diameter of a composite grounding conductor. The authors propose the use of soil-replacing mixtures to reduce soil resistivity and ensure the stability of the grounding loop throughout the entire service life. The proposed method of applying mixtures without pre-drilling makes it possible to reduce the cost of constructing grounding devices.
Advances in Engineering Education with an Analysis of the Effect on the Oil and Gas Industry
Authors: Kirkpatrick C. V. 
Published: December 2024

This paper presents a comprehensive survey of the current status of engineering education in the United States and analyzes the effect of some of the recent advances on high schools, colleges and universities, and industry. It is noted that many constructive and critical appraisals of engineering education in the United States were made several years prior to Sputnik I and that many of the recommendations incident thereto had already been put into effect at leading colleges and universities. A commonly accepted engineering core curricula is given and the dynamics of its content are reviewed. A follow-up survey is presented on changes in engineering education in academic institutions which have occurred since the ASEE "Report on Evaluation of Engineering Education".
It is concluded that, although college and university curricula are still undergoing significant changes, many constructive advances have been made. In general, the engineering graduate of tomorrow will be a more highly creative and imaginative individual-better equipped, academically, to handle the problems presented by our dynamic technology. Industry will face problems created as a result of this rapid transition to maintain its status as one of the most vigorous and dynamic of industries.
A word of caution is voiced against proceeding too rapidly and too far in a direction which may be leading away from our common educational concepts and philosophy in the United States. Although indirect reference is made to Soviet and European engineering education, no direct comparisons are made; however, references to this subject are given.
Amino Acid-Titanium (III) Complex to Control and Synthesize Different Structures of TiO2 Nanoparticles; Usage as a Photocatalysts for Alcohols Oxidization into Aldehyde
Authors: Shad Nazanin Noroozi , Gholizadeh Mostafa , Absalan Yahya , Kopylov Vladimir Viktorovich , Butsov Leonid Alekseevich 
Published: December 2024

Abstract

Different types of amino acids (Glutamine, Glycine, Alanine) were used to coordinate TiCl3 to find the best precursor for synthesizing TiO2. A full investigation was also carried out to synthesize four different structures of TiO2 nanoparticles (TiO2 (A0.8R0.2), TiO2 (A0.6R0.4), TiO2 (Anatase), and TiO2 (Rutile)). Oxidation of alcohol derivatives to their corresponding aldehyde was assessed using the developed nanoparticles as the photocatalyst, under UV light to find the best structure of TiO2. Different physical-chemical analyses were applied to characterize the samples. The results showed that the titanium oxide nanoparticle can be synthesized by the use of glycine at the lowest temperature; thus it was chosen as a precursor to synthesis four different types of TiO2. All the synthesized TiO2 were applied to oxidize benzyl alcohols into benzaldehyde. TiO2 (A0.6R0.4) led to the best efficiency (87%). Hence, it was employed to oxidize benzyl alcohol, 4-cholorobenzyl alcohol, 4-nitrobenzyl alcohol and 4-methoxybenzyl alcohol to their corresponding aldehyde which resulted in the efficiencies of 74, 92, 87, and 65%, respectively.
Fluorescence Imaging-Assisted Thoracoscopic Anatomical Lesion Resection in Treating Congenital Lung Malformation
Authors: He Taozhen , Sun Xiaoyan , Che Guowei , Luo Dengke , Yuan Miao , Yang Gang , Cheng Kaisheng , Xu Chang 
Published: December 2024

Abstract

Identification of lesion demarcation during thoracoscopic anatomical lesion resection is fundamental for treating children with congenital lung malformation. Existing lesion demarcations do not always meet the needs of clinical practice. This study aimed to explore the safety and efficacy of near-infraredfluorescence imaging with nebulized inhalation of indocyanine green for thoracoscopic anatomical lesion resection in children with congenital lung malformation. Under the fluorescence scope, the area of uniform green fluorescence was produced when indocyanine green was distributed into normal lung tissue, and lesions showed little or no green fluorescence, thus delineating a clear fluorescent lesion demarcation. Under the guidance of fluorescent demarcation, all the patients except one case of extralobar sequestration underwent thoracoscopic anatomical lesion resection successfully.
Ante-mortem Diagnosis of Unilateral Pulmonary Vein Stenosis in a Cat: A Case Report
Authors: Aoki Takuma , Miyamoto Takashi , Kizaki Kota , Ueshima Asuka , Iwasaki Kentaro , Kusaka Takuya , Terui Haruko 
Published: December 2024

Abstract

Background: Pulmonary hypertension (PH) in cats may be challenging to detect. Pulmonary venous stenosis (PVS) is rare in cats and can lead to PH. The only reported case of PVS received a postmortem diagnosis. Imaging during the cat's lifetime established the diagnosis in this case.
Case presentation: A 2-year-old Norwegian Forest cat was diagnosed with pulmonary oedema and PH secondary to cor triatriatum sinister(CTS) and showed improved breathing following twice subcutaneous furosemide treatment, 1 and 2 mg/kg, during an overnight stay at the referral veterinary hospital. Sildenafil alone (0.69 mg/kg, PO, BID) was prescribed post-discharge to address PH without diuretics. Following discharge from the referral veterinary hospital, collapse and pre-syncope were suspected to be due to PH. Consequently, sildenafil was titrated weekly, starting at 1.09 mg/kg BID and then increasing to 1.63 mg/kg BID. Pre-syncope and collapse resolved, and pulmonary opacities reduced considerably, although concerns remained that increased pulmonary blood flow to suspected CTS from sildenafil might worsen cardiogenic pulmonary oedema. The patient was also treated with rivaroxaban (2.5 mg/head, SID), considering the increased risk of thrombus formation due to blood flow stasis and endothelial damage. Thirty-eight days later, the cat presented to our hospital for examination. On echocardiography, the continuous mosaic blood flow (maximum and minimum velocity, 3.14 m/s; estimated pressure gradient, 39.4 mmHg) was observed in two enlarged pulmonary veins. Pulmonary artery enlargement (main pulmonary artery to thoracic aorta ratio: 1.90), pulmonary vein stenosis (PVS), and diffuse bilateral ground-glass lung opacities were observed using computed tomography. PH with unilateral PVS involving two out of the three right pulmonary veins, specifically the right cranial and right middle pulmonary veins, along with pulmonary parenchymal disease, was diagnosed. The cat was further treated with furosemide (1 mg/kg, BID, PO) with no clinical signs but succumbed to acute dyspnoea 51 days after the first visit.
Conclusions: Unilateral PVS should be considered in young cats with a localised alveolar pattern, as the prognosis may be poor. Severe PH with PVS may coexist with lung disease. If sildenafil is used, it should be started at a low dose and monitored closely.
Development of a Nomogram for Predicting Malignancy in BI-RADS 4 Breast Lesions Using Contrast-Enhanced Ultrasound and Shear Wave Elastography Parameters
Authors: Ren Tiantian , Gao Zhenzhen , Yang Lufeng , Cheng Weibo , Luo Xiao 
Published: December 2024

Abstract

Background
This study aimed to construct a Least Absolute Shrinkage and Selection Operator (LASSO) logistic regression (LR) model using Shear Wave Elastography (SWE) and Contrast-Enhanced Ultrasound (CEUS) quantitative imaging features to accurately assess the malignant probability of Breast Imaging Reporting and Data System (BI-RADS) 4 breast lesions (BLs).
Methods
Female patients (n = 111) with BI-RADS 4 BLs detected via routine ultrasound at Ma'anshan People's Hospital underwent SWE, CEUS, and histopathological examinations. Histopathological results served as the benchmark. LASSO LR analysis with 10-fold cross-validation identified significant imaging features for malignancy prediction. A predictive nomogram was developed and validated with bootstrap sampling. Performance was assessed using calibration curves, Receiver Operating Characteristic (ROC) analysis, and decision curve analysis (DCA).
Results
Histopathological analysis revealed 35 malignant and 76 benign BLs. Significant features included peak intensity (PI) from CEUS (odds ratio [OR] = 5.788, p < 0.05), the area under the curve (AUC) from CEUS (OR = 6.920, p < 0.05), and Maximum Young’s Modulus (SWE_Max) from SWE (OR = 10.802, p < 0.05). The nomogram showed an AUC of 0.875 (95% CI: 0.805–0.945), sensitivity of 0.886, and specificity of 0.684, with good calibration and clinical utility.
Conclusion
The nomogram outperformed traditional BI-RADS methods, providing excellent predictive performance for distinguishing malignant from benign BI-RADS 4 BLs and reducing unnecessary biopsies.
Exploration of treatment burden through examination of workload and patient capacity during transition onto kidney replacement therapy: a systematic review of qualitative research
Authors: Jones Catrin , Cairns Ross , Walker Heather , Welsh Silje , Edgar Benjamin , Stevenson Karen , Jani Bhautesh D. , Mark Patrick B. , Kingsmore David , Gallacher Katie I. 
Published: December 2024

Abstract

Background:
Patients with advanced chronic kidney disease requiring initiation of Kidney Replacement Therapy (KRT) are frequently asked to enact complex management plans. Treatment burden has been defined as the effect of healthcare workload and the capacity a person has to manage this workload has on wellbeing. The aim of this review is to examine the experience of healthcare workload and the factors that affect capacity to meet that workload for people transitioning onto KRT for the first time, using a framework synthesis of published literature informed by Normalisation Process Theory (NPT) and Theory of Patient Capacity (TPC).
Methods: Medline, Scopus and CINAHL were systematically searched with manual citation and reference searching. Studies were included if meeting the criteria of adults aged 18 or over transitioning for the first time onto any modality of KRT (haemodialysis, peritoneal dialysis or kidney transplantation), using qualitative methodologies to describe any aspect of experiences of healthcare workload or any factors that affect capacity to manage workload were included. Abstracts and full papers were independently screened by two reviewers and data extraction and quality appraisal were also independently conducted by two reviewers. Qualitative data were analysed using framework synthesis informed by NPT and TPC.
Results: 24,380 studies were screened, 406 full texts were reviewed and 18 studies were included. There were four broad categories of workload described: making sense of KRT, working out what to do and how to do it, meeting the challenges of KRT, and reflecting on work done. Patient capacity influenced the experience of all types of workload and the treatment burden generated by the work.
Conclusion: Transitioning onto KRT is a period of very high healthcare workload and potentially high treatment burden. The relationship between healthcare workload and capacity to handle workload is complex, multifactorial and changes over time. By better understanding workload, capacity and burden during transition we can develop better ways of measuring these important aspects of care and develop interventions to reduce treatment burden in those transitioning onto KRT.
RvD1 Combined with Exercise Promotes Mitophagy and Reduces Neuronal Apoptosis in Mice After Intracerebral Hemorrhage via the BDNF/TrkB/PI3K/AKT Pathway
Authors: Lv Xiaoyu , Dandan Li , Tianzhao Ouyang , Ziyou Zhang , Zhuang Li , Mingrui Liu , Yusong He , Yangyang Zhong , Yanjiao Li , Chun Shi , Siqi Wang , Tong Li , Bensi Zhang 
Published: December 2024

Abstract

Resolvin D1 (RvD1) is an endogenous anti-inflammatory mediator that can modulate the inflammatory response and promote inflammation resolution. RvD1 has been shown to exert neuroprotective effects in various central nervous system contexts; however, its role in the pathophysiological processes of Intracerebral hemorrhage (ICH) and the potential protective mechanisms when combined with exercise rehabilitation training remain unclear. In this study, we aimed to investigate whether RvD1 combined with exercise rehabilitation training could protect against neurological deficits in ICH mice by activating the BDNF/TrkB/PI3K/AKT signaling pathway, improve cognitive function, reduce neuronal apoptosis and inflammatory response, and explore its relationship with mitochondrial autophagy. A mouse model of ICH was established using collagenase, and treatment with RvD1 combined with three weeks of exercise rehabilitation significantly improved neurological deficits, muscle strength, learning and memory in the cerebral hemorrhage mice while reducing anxiety-like behavior. RvD1 combined with exercise rehabilitation training can up-regulate anti-inflammatory factors, inhibit inflammatory state and activate BDNF/TrkB/PI3K/ akt related pathway.TUNEL staining confirmed a decrease in residual apoptotic neurons, while transmission electron microscopy showed an increase in mitochondrial autophagosomes with combined treatment. Mendelian randomization and molecular docking further confirmed the association of RvD1 with targets related to mitophagy and inflammatory factors, thereby clarifying the mechanism of RvD1 involvement.In summary, RvD1 combined with exercise rehabilitation can activate the BDNF/TrkB/PI3K/AKT signaling pathway, effectively reduce neuronal apoptosis and inflammatory responses following ICH in mice, and participate in mitochondrial autophagy-related states. This comprehensive therapeutic strategy promotes neurological recovery and provides insights for the clinical management of this condition.
Crystal structure analysis of four 2-pyridineformamide thiosemicarbazone derivatives using non-spherical atomic form factors
Authors: Shakya Bhushan , Mahiya Kuldeep , Shrestha Ramina Maharjan , Oli Hari Bhakta , Yadav Paras Nath 
Published: December 2024

Abstract

The crystal structures of four 2-pyridineformamide thiosemicarbazone derivatives viz, (Z,Z’)-4-methyl-N'-(morpholine-4-carbonothioyl)picolinohydrazonamide (1), (Z,Z’)-4-methyl-N'-(thiomorpholine-4-carbonothioyl) picolinohydrazonamide (2) and (Z,Z’)-6-methyl-N'-(thiomorpholine-4-carbonothioyl)picolinohydrazonamide (3) and (Z,Z’)-N'-(azepane-1-carbonothioyl)-4-methylpicolinohydrazonamide (4) has been determined by single crystal X-ray diffraction analysis. Combined quantum mechanical methods (ORCA) and computation of non-spherical scattering form factors (NoSpherA2) were used for the refinement with anisotropic hydrogen atoms. The results of using independent atom model (IAM) and Hirshfield atom refinement (HAR) method based R2SCAN/ def2-TZVP functional were compared. All compounds show an improvement in the model after HAR refinement compared to independent atom model (IAM). There is a significant decrease in the residual factors with the use of anisotropic hydrogen refinement. HAR refinement provides a clearer understanding of electron delocalization, lone pairs, and the accumulation and depletion of electron density. The information obtained came from standard resolution single crystal X-ray diffraction data, indicating that high-quality structural analysis can now be performed without the need for neutron or synchrotron facilities.
One sentence essence:
Hirshfield atom refinement provides a clearer understanding of electron delocalization, lone pairs, and the accumulation and depletion of electron density.
Cerebral Venous Thrombosis Presenting as Lower Limb Action Myoclonus: A Case Report
Authors: Osayan Andre Anthony A. , Autentico Alicia Rose T. , Villamor Reynaldo Benedict V. , Saranza Gerard Raimon M. 
Published: December 2024

Abstract

Introduction:
Cerebral venous thrombosis is a rare stroke caused by occlusion of venous sinuses. Most cases usually present with headaches, seizures, or focal deficits, but rarely as movement disorders.
Case Report:
Herein we describe a 57-year-old female who presented with action myoclonus in the right lower extremity. Venous thrombosis involving the straight and inferior superior sagittal sinuses was noted on CT angiography. She was then treated with Apixaban and Levetiracetam, with good control of the myoclonic jerks. However, she later developed an action myoclonus and weakness of the left leg due to venous infarction. Cerebral angiography showed clearing of thrombosis in the previously affected sinuses. Workup for prothrombotic conditions predisposing to venous thrombosis was unremarkable. Anticoagulation with Warfarin and a higher dose of Levetiracetam eventually afforded a gradual improvement of the weakness and complete resolution of her myoclonus.
Conclusion
Although rare, cerebral venous sinus thrombosis should also be considered as an acquired cause of action myoclonus.
The Impact of Family Care on Sleep Quality in Perimenopausal Women: Mediating Roles of Anxiety and Depression
Authors: Liang Nana , Zhao Juan , Ren JiShun , Shi Xinyi , Zheng Haixia 
Published: December 2024

Abstract

Background
Family systems theory posits that the family serves as the most intimate social support network, offering patients practical care, companionship, and emotional communication. Effective family functioning can significantly enhance patients' sleep quality. This study aimed to investigate the effects of family care on sleep quality and its mechanism of action in perimenopausal women in Gansu Province, and to provide theoretical support for alleviating anxiety and depression levels and improving sleep quality in perimenopausal women.
Methods
Perimenopausal women (n = 860) in Gansu Province were surveyed using the Self-Rating Scale of Sleep, Self-Rating Anxiety Scale, Self-Rating Depression Scale, and Family Adaptation Partnership Growth Affection Resolve.
Results
The sleep quality, anxiety, and depression scores of perimenopausal women in Gansu Province were 25.33 ± 5.00, 40.74 ± 10.89, and 40.77 ± 10.03, respectively, and the family care score was 6.64 ± 2.36. Family care was negatively correlated with sleep quality, anxiety, and depression scores; anxiety was positively correlated with depression and sleep quality scores, and depression was positively correlated with sleep quality scores. Anxiety and depression in perimenopausal women partially mediated family care and sleep quality, with mediating effects accounting for 15.74% and 60.69% of the total effect, respectively.
Conclusion
Family care has a direct predictive effect on sleep quality in perimenopausal women, and anxiety and depression mediate the relationship between family care and sleep quality. Lowering anxiety and depression levels in perimenopausal women is an effective way to improve sleep quality.