bluuebunny/crossref_metadata_2025_split huggingface dataset:
Highly Multiplexed Tissue Imaging in Precision Oncology and Translational Cancer Research
Authors: Bollhagen Alina , Bodenmiller Bernd 
Published: November 2024

Abstract
Precision oncology tailors treatment strategies to a patient’s molecular and health data. Despite the essential clinical value of current diagnostic methods, hematoxylin and eosin morphology, immunohistochemistry, and gene panel sequencing offer an incomplete characterization. In contrast, highly multiplexed tissue imaging allows spatial analysis of dozens of markers at single-cell resolution enabling analysis of complex tumor ecosystems; thereby it has the potential to advance our understanding of cancer biology and supports drug development, biomarker discovery, and patient stratification. We describe available highly multiplexed imaging modalities, discuss their advantages and disadvantages for clinical use, and potential paths to implement these into clinical practice.
Significance: This review provides guidance on how high-resolution, multiplexed tissue imaging of patient samples can be integrated into clinical workflows. It systematically compares existing and emerging technologies and outlines potential applications in the field of precision oncology, thereby bridging the ever-evolving landscape of cancer research with practical implementation possibilities of highly multiplexed tissue imaging into routine clinical practice.
Early Cancer Detection Through Comprehensive Mapping of Dynamic Tumorigenesis
Authors: Chang Jiang , Zheng Tongsen , Wu Chen 
Published: November 2024

Summary:
Current strategies for early cancer detection and diagnosis need updating to achieve greater precision, necessitating the creation of a comprehensive evolutionary map of tumorigenesis. This requires establishing high-quality prospective cohorts, systematically collecting samples for integrated spatiotemporal multiomics analyses, and efficiently translating laboratory findings into clinical applications.
Targeting Noncoding cis-Regulatory Elements for Cancer Therapy in the Context of the 3D Genome
Authors: Li Kailong , Wei Gong-Hong , Yin Yuxin , Feng Jiawen 
Published: November 2024

Summary:
Significant efforts have been made to identify and validate oncoproteins and ncRNAs as therapeutic targets for cancer therapy; however, emerging observations suggest that noncoding cis-regulatory elements, which orchestrate the 3D organization of the genome and thus the transcriptional landscape, are potential therapeutic targets as well. In this commentary, we envisage that further efforts to decipher the noncoding cis-regulatory code and performing systematic surveys of functional noncoding cis-regulatory elements and recurrent 3D genome alterations in both cancerous and nonmalignant cells within tumor tissues will pave the way to the development of novel therapeutic strategies.
A Call for Innovative Translational and Clinical Research to Address China’s Unique Cancer Landscape
Authors: Zhang Chaoqi , Wu Peng , Li Dongyu , Gu Xuanyu , Lin Chuqi , Zhou Junhan , Shang Dexin , Liu Jingjing , Ma Ruijie , Zhao Bohui , Sun Nan , He Jie 
Published: November 2024

Summary:
The Chinese government has, in recent decades, implemented various administrative laws and regulatory policies to expedite cancer therapeutic development, boosting research and development pipelines for domestic pharmaceutical companies and clinical trials; however, China faces unique challenges given the high prevalence of certain cancer types and distinct disease burdens, some of which are frequently overlooked by international pharmaceutical companies. Given the substantial unmet need for China-specific cancer care, it is crucial to promote the development of innovative pharmaceutical and clinical research in China, with a particular emphasis on addressing tumors most prevalent in its population.
Combination Diagnostics: Adding Blood-Based ctDNA Screening to Low-Dose CT Imaging for Early Detection of Lung Cancer
Authors: Haber Daniel A. , Skates Steven J. 
Published: November 2024

Summary:
Annual low-dose CT screening of individuals with a smoking history identifies early curable lung tumors and reduces cancer mortality by 20%, yet only a minority of eligible patients undergo such monitoring. Mazzone and colleagues apply a blood-based cfDNA fragmentomic assay as a high-sensitivity/low-specificity pre-screen to help stratify individuals who may benefit most from more definitive low-dose CT imaging.
See related article by Mazzone et al., p. 2224
Evolvability in Artificial Development of Large, Complex Structures and the Principle of Terminal Addition
Authors: Fontana Alessandro , Wróbel Borys 
Published: November 2024

Abstract
Epigenetic tracking (ET) is a model of development that is capable of generating diverse, arbitrary, complex three-dimensional cellular structures starting from a single cell. The generated structures have a level of complexity (in terms of the number of cells) comparable to multicellular biological organisms. In this article, we investigate the evolvability of the development of a complex structure inspired by the “French flag” problem: an “Italian Anubis” (a three-dimensional, doglike figure patterned in three colors). Genes during development are triggered in ET at specific developmental stages, and the fitness of individuals during simulated evolution is calculated after a certain stage. When this evaluation stage was allowed to evolve, genes that were triggered at later stages of development tended to be incorporated into the genome later during evolutionary runs. This suggests the emergence of the property of terminal addition in this system. When the principle of terminal addition was explicitly incorporated into ET, and was the sole mechanism for introducing morphological innovation, evolvability improved markedly, leading to the development of structures much more closely approximating the target at a much lower computational cost.
Attentional Refreshing in Working Memory and Its Interplay with Long-term Memory: A Behavioral and Electroencephalographic Study
Authors: Labaronne Maximilien , Caclin Anne , Plancher Gaën 
Published: November 2024

Abstract
Despite the growing interest in the study of attentional refreshing, the functioning of this working memory maintenance mechanism, including its cerebral underpinnings, is still debated. In particular, it remains unclear whether refreshing promotes long-term memory and whether it, in return, depends on long-term memory content to operate. Here, we used direct maintenance instructions and measured brain activity to investigate working memory maintenance with two objectives: (1) test if different behavioral and oscillatory patterns could be observed when participants were instructed to use attentional refreshing versus verbal rehearsal, and (2) observe whether and how refreshing is modulated when maintaining novel (pseudowords) versus familiar (words) memoranda. We conducted an EEG experiment using a modified Brown-Peterson task, in which we manipulated the type of maintenance engaged through explicit instructions (verbal rehearsal vs. refreshing), the type of memoranda (words vs. pseudowords), and the memory load (2 vs. 6). Using scalp EEG, we measured both neural oscillations during working memory maintenance and ERPs during the concurrent parity judgment task. For words, we showed that verbal rehearsal benefited more short-term recall whereas refreshing benefited more delayed recall. In keeping with these behavioral differences between maintenance instructions, frontal–midline theta power increased with memory load only when using verbal rehearsal, whereas occipito-parietal alpha desynchronization was larger with refreshing than verbal rehearsal. When maintaining pseudowords, verbal rehearsal also benefitted short-term recall more than refreshing. However, no long-term memory benefit of refreshing was observed for pseudowords, and oscillatory activity was not different under the two maintenance instructions. Our results provide new evidence supporting the independence between attentional refreshing and verbal rehearsal, and bring new insight into refreshing functioning. We discuss the possible interpretations of these results and the implications for the attentional refreshing literature.
Finger Tapping as a Behavioral Indicator of Presence in VR
Authors: Hinkle Sean , Soares Shayna , Bohil Corey 
Published: November 2024

Abstract
The sense of spatial presence in virtual environments (VEs) is typically measured using self-report methods after task completion. Recent years have seen efforts to measure presence via physiological markers, with mixed results. Behavioral indicators might better track variations in presence over time. We propose finger tapping as an indicator of changing attentional demands corresponding to low and high presence conditions in VEs. Participants completed “virtual plank” (raised vs lowered to reflect high or low presence) and VR video (90° vs 360°) conditions while attempting to tap their fingers at a specified rate. Participants wore a data glove to measure finger taps, and we analyzed variations in the inter-tap intervals. We trained a neural net classifier to distinguish between low and high presence conditions using features derived from the positional finger-tap timeseries (e.g., power spectral density, autocorrelation). The trained network classified test epochs with a success rate of 80% for both video and plank conditions.
Make it a standard? The creation and variability assessment of a consensus standard protocol for Tenebrio molitor larvae feeding trials
Authors: Deruytter D. , Rumbos C.I. , Adamaki-Sotiraki C. , Tournier L. , Ageorges V. , Coudron C.L. , Yakti W. , Ulrichs C. , Spranghers T. , Berrens S. , Van Peer M. , Bellezza Oddon S. , Biasato I. , Resconi A. , Paris N. , Hotte N. , Hénault-Ethier L. , Gasco L. , Athanassiou C.G. 
Published: November 2024

Abstract
Interest in the nutrition of the yellow mealworm (Tenebrio molitor L.) larvae is on the rise, leading to an increase in publications on this topic. The absence of a standard protocol and resulting differences in experimental designs reduces comparability among studies and impedes research on mealworm nutrition. To address this, a consensus standardised protocol was developed specifically for the evaluation of mealworm larval growth and performance in feeding trials. The efficacy of this protocol was evaluated through an international ring test involving seven partners using two wheat brans as dry feed (a standard bran and a local bran) at 27 °C and 60% relative humidity. As experimental units, plastic crates filled with 2.1 kg of bran and 10,000 4-week-old larvae were used with six replicates. Agar gel was provided as wet feed ad libitum. The mean individual larval weight and the number of larvae per crate were determined weekly until either three or more replicates ran out of feed or pupation exceeded 10%. At harvest, the total larval fresh biomass and amount of frass was determined. Larval samples were taken for chemical analysis. To assess the protocol, the within (repeatability) and between (reproducibility) laboratory variability was calculated for each parameter. The repeatability was good (limit at 12% (standard) and 14% (local)). The reproducibility was poorer with a limit 2.7 times higher for the standard feed (36%) and 3.8 times higher for the local feed (55%). For both feeds, the total larval fresh harvest, amount of frass and the larval protein concentration were the most consistent both within and among laboratories. The highest variability was observed at the early life stages and for the larvae ash content. The detailed consensus standard protocol and repeatability/reproducibility estimates can be used as basis for future mealworm feeding trials, comparing results and future improvements.
Revictimization of women victims of intimate partner violence in the French press: A social representations approach
Authors: Ortega Pacheco Yesid José , Le Barbenchon Emmanuelle 
Published: November 2024

Objective: This study aims to analyze how the press portrays cases of revictimization experienced by women in France, through the lens of social representation theory. Method: An exploratory, descriptive study was conducted using a corpus of 157 online press articles. A total of 187,773 words and 5,240 segments were analyzed using Iramuteq version 7, employing top-down hierarchical classification and lexical similarity analysis. Results: The analysis identified four classes: 1. Violent incidents (25.5%); 2. Political actions against violence (26%); 3. Institutional revictimization (26.6%) and 4. Violence and breaking the silence (21.9%). Conclusions: The findings illustrate how media portrayals contribute to the construction of social representations surrounding revictimization. Class 1 reveals a focus on extreme cases of violence, such as femicides, and highlights a potential gender bias in media reporting through the omission of terms like ‘femicide’. Class 2 demonstrates the influence of ideological perspectives on the portrayal of political measures against violence, with conservative outlets framing such actions within traditional values, while progressive newspapers advocate for systemic reform. Class 3 shows a gap in the conceptualization of institutional revictimization in the French press compared to other countries, revealing how conservative portrayals can minimize systemic issues and perpetuate harmful stereotypes. Class 4 shows the role of female journalists in addressing various forms of violence, despite the limited impact of the #MeToo movement in recent coverage. Collectively, these findings elucidate how media representations shape public attitudes and institutional responses to gender-based violence through the lens of social representations.
Ecofriendly hybrid natural fiber reinforced polypropylene composites from biowastes
Authors: Techawinyutham Laongdaw , Techawinyutham Wiroj , Ayyappan Vinod , Sanjay Mavinkere Rangappa , Siengchin Suchart 
Published: November 2024

The used ground coffee waste (GCW) and tea leaf waste (TLW), the large wastes from the beverage process, were selected as natural reinforcement and natural pigment in the polypropylene (PP) matrix. In this work, the maleic anhydride polypropylene (MAPP) was added to the composites to develop the compatibility between fillers, fibers, and polymer matrix. The TLW/PP composites, GCW/PP composites, and GCW/TLW/PP hybrid composites were prepared in the ratio of TLW and GCW ranging from 0 to 30 wt% with the addition of MAPP at 5 wt% of fillers. The presence of GCW and TLW in the PP matrix decreased tensile properties and impact resistance but increased flexural modulus and hardness of PP at high concentrations of GCW and TLW. The increase in GCW and TLW concentration slightly reduced melting temperature but it enhanced the tensile and flexural modulus. Furthermore, it enhanced the hardness, thermal and rheological properties of the composites. The TLW/PP composites had better mechanical properties, and rheological properties than GCW/PP composites but thermal properties were quite similar. The mechanical properties of GCW/TLW/PP hybrid composites were better than those of GCW/PP composites but lower than those of TLW/PP composites. The rheological and thermal behaviour of hybrid composites were like that of TLW/PP composites. Incorporating MAPP improves the mechanical and rheological behavior of PP composites by enhancing the interaction among PP, GCW, and TLW. However, this addition does not significantly enhance the composites’ thermal properties. The optimum composite was hybrid composites of GCW/TLW/PP consisting of GCW of 10 wt%, TLW of 20 wt%, and MAPP of 1.5 wt% which presented good mechanical, thermal, and rheological properties. The mechanical properties of this hybrid composites were as follows: a tensile strength of 26.35 MPa, elongation at break of 10.91%, tensile modulus of 636.69 MPa, flexural strength of 45.57 MPa, flexural modulus of 3632.86 MPa, impact resistance of 12.72 kJ/m2, and a Shore D hardness of 76.15. The overall results showed that GCW and TLW can be used as reinforcement material and natural pigment in the polymer matrix.
Understanding the help-seeking behaviours of women at risk of postnatal depression
Authors: Siak Junpei Elizabeth , Wang Biwen Jemie , Chua Tze-Ern , Ch’ng Ying Chia , Chen Helen 
Published: November 2024

Background: Postnatal depression, which affects between 10 and 25% of women worldwide, is a major public health problem. However, studies have shown that patients with postnatal depression engage poorly with treatment. Objectives: This qualitative study aims to examine the help-seeking behaviours amongst women who were screened to be at risk of postnatal depression in a public maternity hospital. Methods: From April 2008 to January 2010, 214 women who returned to a public maternity hospital for their postnatal obstetric check-ups were screened to be at risk of postnatal depression, with scores of 13 and above on the Edinburgh Postnatal Depression Scale (EPDS). Of these, 48 women agreed to participate in an open-ended phone interview which explored facilitators and barriers to help-seeking. The interviews were audio-recorded, transcribed and analysed to identify recurrent themes. Results: Three overarching themes and corresponding sub-themes were identified: personal motivations for seeking treatment (wish to be a better mother, desire for emotional support, wish for diagnosis), external facilitators for seeking treatment (family support, referral by obstetrician, postnatal emotional health screening) and barriers to seeking treatment (practical concerns, stigma, lack of knowledge about postnatal depression). Conclusions: The barriers and facilitators reported by study participants were consistent with those in existing literature, though participants’ personal motivations for seeking treatment have not been widely reported. Potential interventions to encourage help-seeking in women at risk of postnatal depression should address barriers and facilitators of help-seeking, and women’s personal motivations for seeking treatment should be explored in greater detail.
Genetic characteristics and antimicrobial resistance of Staphylococcus aureus isolates from pig farms in Korea: emergence of cfr-positive CC398 lineage
Authors: Lee Jun Bong , Lim Ji Hyun , Park Ji Heon , Lee Gi Yong , Park Kun Taek , Yang Soo-Jin 
Published: November 2024

Abstract
Background
Livestock-associated Staphylococcus aureus (LA-SA) has gained global attention because of its ability to colonize farm animals and transmit to the environment and humans, leading to symptomatic infections and the spread of antimicrobial resistance (AMR). In the last decade, numerous studies have reported a high prevalence of S. aureus clonal complex (CC) 398 in pig farms.

Results
In this study, 163 S. aureus isolates were collected from healthy pigs (n = 110), farm environments (n = 42), and farm workers (n = 11), and their AMR profiles and epidemiological characteristics were analyzed. We identified 51 (31.3%) methicillin-resistant S. aureus (MRSA) and 112 (68.7%) methicillin-susceptible S. aureus (MSSA), with 161 (98.8%) isolates belonging to the CC398 lineage. The highest prevalence of spa type t571 was observed among the CC398 isolates. All 47 sequence type (ST) 398 MRSA isolates carried staphylococcal cassette chromosome mec (SCCmec) V, while four ST541 isolates carried SCCmec IV. High levels of resistance to commonly used antibiotics, including phenicols, quinolones, lincosamides, macrolides, aminoglycosides, and tetracyclines, have been observed on Korean pig farms. Notably, 21 cfr-positive CC398 isolates (four ST541-SCCmec IV MRSA and 17 ST398 MSSA) displaying increased resistance to linezolid were identified in healthy pigs.

Conclusions
In summary, these findings suggest that the multidrug-resistant CC398 S. aureus lineage predominantly colonizes healthy pigs and farm environments in Korea. The emergence of cfr-positive S. aureus at human-animal interfaces presents a significant threat to food safety and public health.
Sensory processing and child appetitive traits: findings from the ROLO longitudinal birth cohort study
Authors: Delahunt Anna , Callanan Sophie , O’ Brien Eileen C. , Geraghty Aisling A. , O’ Reilly Sharleen L. , McDonnell Ciara M. , Hokey Emma , McAuliffe Fionnuala M. 
Published: November 2024

Abstract
Background
Oral sensory hypersensitivity has been linked with fussy eating predominantly in non-typically developing children. We hypothesized that child ‘Oral’ (touch, smell and taste in the mouth) and ‘Social-Emotional’ (response to social expectations) sensory processing are associated with child appetitive traits in typically developing preteen children. Additionally, we explored relationships between maternal sensory profiles and their offspring’s sensory profile.

Methods
This is secondary analysis of 130 mother-child dyads from the 9–11-year-old follow-up of the ROLO longitudinal birth cohort study. The Dunn Sensory Profile (Adolescent/Adult) and the Dunn Child Sensory Profile 2 were used to assess maternal and child sensory profiles, respectively. The Children’s Eating Behaviour Questionnaire was used to assess child appetitive traits. Multiple linear regression examined associations between ‘Oral’ and ‘Social-Emotional’ segments of the child sensory profile and child appetitive traits. Associations between maternal and child sensory profiles were examined using sensory profile quadrants (Dunn’s sensory processing framework). These refer to four distinct patterns of sensory processing that indicate how an individual responds to sensory input.

Results
In total, 130 mother-child dyads were included. In adjusted analysis child ‘Oral’ sensory processing was associated with higher mean scores in the appetitive traits ‘Desire to Drink’ (B = 0.044, 95% CI = 0.025,0.062) and ‘Food Fussiness’ (B = 0.080, 95% CI = 0.059,0.101) and with lower mean scores in ‘Enjoyment of Food’ (B=-0.038, 95% CI -0.055,-0.022). Child ‘Social-Emotional’ responses were associated with higher mean scores in ‘Desire to Drink’ and ‘Food Fussiness’. Higher scores in the maternal sensory profile quadrants of ‘Sensitivity’, ‘Avoiding’ and ‘Registration’ were positively associated with higher scores in the corresponding child sensory profile quadrants.

Conclusion
Our findings suggest that in typically developing children presenting with fussy eating, oral sensory hypersensitivity and higher scores in social-emotional responses to sensory processing may be an underlying determinant. The relationship observed between ‘Oral’ and ‘Social-Emotional’ sensory processing segments and ‘Desire to Drink’ requires further research to ascertain the type of drinks being consumed and how this impacts appetite. Further research is also required to explore the influence of maternal sensory profile on their offsprings response to sensory input.