bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prehospital Blood Product Administration Opportunities in Ground Transport ALS EMS – A Descriptive Study
Authors: Mix Felicia M. , Zielinski Martin D. , Myers Lucas A. , Berns Kathy S. , Luke Anurahda , Stubbs James R. , Zietlow Scott P. , Jenkins Donald H. , Sztajnkrycer Matthew D. 
Published: April 2018

AbstractIntroductionHemorrhage remains the major cause of preventable death after trauma. Recent data suggest that earlier blood product administration may improve outcomes. The purpose of this study was to determine whether opportunities exist for blood product transfusion by ground Emergency Medical Services (EMS).MethodsThis was a single EMS agency retrospective study of ground and helicopter responses from January 1, 2011 through December 31, 2015 for adult trauma patients transported from the scene of injury who met predetermined hemodynamic (HD) parameters for potential transfusion (heart rate [HR]≥120 and/or systolic blood pressure [SBP]≤90).ResultsA total of 7,900 scene trauma ground transports occurred during the study period. Of 420 patients meeting HD criteria for transfusion, 53 (12.6%) had a significant mechanism of injury (MOI). Outcome data were available for 51 patients; 17 received blood products during their emergency department (ED) resuscitation. The percentage of patients receiving blood products based upon HD criteria ranged from 1.0% (HR) to 5.9% (SBP) to 38.1% (HR+SBP). In all, 74 Helicopter EMS (HEMS) transports met HD criteria for blood transfusion, of which, 28 patients received prehospital blood transfusion. Statistically significant total patient care time differences were noted for both the HR and the SBP cohorts, with HEMS having longer time intervals; no statistically significant difference in mean total patient care time was noted in the HR+SBP cohort.ConclusionsIn this study population, HD parameters alone did not predict need for ED blood product administration. Despite longer transport times, only one-third of HEMS patients meeting HD criteria for blood administration received prehospital transfusion. While one-third of ground Advanced Life Support (ALS) transport patients manifesting HD compromise received blood products in the ED, this represented 0.2% of total trauma transports over the study period. Given complex logistical issues involved in prehospital blood product administration, opportunities for ground administration appear limited within the described system.MixFM, ZielinskiMD, MyersLA, BernsKS, LukeA, StubbsJR, ZietlowSP, JenkinsDH, SztajnkrycerMD. Prehospital blood product administration opportunities in ground transport ALS EMS – a descriptive study. Prehosp Disaster Med. 2018;33(3):230–236.
Needle Thoracostomy: Does Changing Needle Length and Location Change Patient Outcome?
Authors: Weichenthal Lori A. , Owen Scott , Stroh Geoffory , Ramos John 
Published: April 2018

AbstractBackgroundNeedle thoracostomy (NT) is a common prehospital intervention for patients in extremis or cardiac arrest due to trauma. The purpose of this study is to compare outcomes, efficacy, and complications after a change in policy related to NT in a four-county Emergency Medical Services (EMS) system with a catchment area of greater than 1.6 million people.MethodsThis is a before and after observational study of all patients who had NT performed in the Central California (USA) EMS system. The before, anterior midclavicular line (MCL) group consisted of all patients who underwent NT from May 7, 2007 through February 28, 2013. The after, midaxillary line (MAL) axillary group consisted of all patients who underwent NT from March 1, 2013 through January 30, 2016, after policy revisions changed the timing, needle size, and placement location for NT. All prehospital and hospital records where NT was performed were queried for demographics, mechanism of injury, initial status and post-NT clinical change, reported complications, and final outcome. The trauma registry was accessed to obtain Injury Severity Scores (ISS). Information was manually abstracted by study investigators and examined utilizing univariate and multivariate analyses.ResultsThree-hundred and five trauma patients treated with NT were included in this study, of which, 169 patients (the MCL group) were treated with a 14-guage intravenous (IV) catheter at least 5.0-cm long at the second intercostal space (ICS), MCL after being placed in the ambulance; and 136 patients (the MAL group) were treated with a 10-guage IV catheter at least 9.5-cm long at the fifth ICS, MAL on scene. The mean ISS was lower in the MAL cohort (64.5 versus 69.2; P=.007). The mortality rate was 79% in both groups. The multivariate model with regard to survival supported that a lower ISS (P<.001) and reported clinical change after NT (P=.003) were significant indicators of survival. No complications from NT were reported.ConclusionsChanging the timing, length of needle, and location of placement did not change mortality in patients requiring NT. Needle thoracostomy was used more frequently after the change in policy, and the MAL cohort was less injured. No increase in reported complications was noted.WeichenthalLA, OwenS, StrohG, RamosJ. Needle thoracostomy: does changing needle length and location change patient outcome?Prehosp Disaster Med. 2018;33(3):237–244.
Does a modified mindfulness-based cognitive therapy (MBCT) course have the potential to reduce stress and burnout in NHS GPs? Feasibility study
Authors: Hamilton-West Kate , Pellatt-Higgins Tracy , Pillai Neil 
Published: April 2018

AbstractAimTo explore, for the first time, whether a modified mindfulness-based cognitive therapy (MBCT) course has the potential to reduce stress and burnout among National Health Service (NHS) General Practitioners.BackgroundThere is a crisis of low morale among NHS GPs, with most describing their workload as ‘unmanageable’. MBCT has been demonstrated to improve stress and burnout in other populations, but has not yet been evaluated in a cohort of NHS GPs.MethodsNHS GPs in South East England (n=22) attended a modified version of the MBCT course approved by National Institute for Health and Care Excellence for prevention of depressive relapse. This comprised eight weekly 2-h sessions with homework (mindfulness practice) between sessions. Participants completed the Maslach Burnout Inventory (MBI) and Perceived Stress Scale (PSS) before (baseline) and then again one month (T2) and three months (T3) after attending the course. We also obtained qualitative data on participants’ experiences of the course.FindingsCompliance with the intervention was very high. All GPs attended at least six sessions and all completed baseline questionnaires. At T2, data were obtained from 21 participants (95%); PSS scores were significantly lower than at baseline (P<0.001), as were MBI emotional exhaustion (P<0.001) and depersonalization scores (P=0.0421). At T3 we obtained data for 13 participants (59%); PSS scores and MBI emotional exhaustion scores were significantly lower (P<0.001; P=0.0024, respectively) and personal accomplishment scores were significantly higher (P<0.001) than at baseline. Participants reported that the course helped them to manage work pressures, feel more relaxed, enjoy their work and experience greater empathy and compassion (for self, colleagues and patients). Findings of this preliminary evaluation are promising. Further research is needed to evaluate this approach within a larger randomized-controlled trial.
Machine Learning Predictions of a Multiresolution Climate Model Ensemble
Authors: Anderson Gemma J. , Lucas Donald D. 
Published: April 2018

AbstractStatistical models of high‐resolution climate models are useful for many purposes, including sensitivity and uncertainty analyses, but building them can be computationally prohibitive. We generated a unique multiresolution perturbed parameter ensemble of a global climate model. We use a novel application of a machine learning technique known as random forests to train a statistical model on the ensemble to make high‐resolution model predictions of two important quantities: global mean top‐of‐atmosphere energy flux and precipitation. The random forests leverage cheaper low‐resolution simulations, greatly reducing the number of high‐resolution simulations required to train the statistical model. We demonstrate that high‐resolution predictions of these quantities can be obtained by training on an ensemble that includes only a small number of high‐resolution simulations. We also find that global annually averaged precipitation is more sensitive to resolution changes than to any of the model parameters considered.
Anomalous Complex Electrical Conductivity of a Graphitic Black Schist From the Himalayas of Central Nepal
Authors: Börner Jana H. , Girault Frédéric , Bhattarai Mukunda , Adhikari Lok Bijaya , Deldicque Damien , Perrier Frédéric , Spitzer Klaus 
Published: April 2018

AbstractWe analyzed in the laboratory the frequency‐dependent, complex‐valued, electrical conductivity of a graphitic black schist and an augen gneiss, both collected in the Main Central Thrust shear zone in the Himalayas of central Nepal, which was heavily affected by the deadly Mw7.8 Gorkha earthquake in 2015. We focused on anisotropy and salinity dependence of both cores and crushed material as well as the impact of CO2 on conductivity. This black schist possesses an extraordinarily high polarizability and a highly frequency‐dependent conductivity. Its anisotropy is very pronounced. The investigations can relate the main polarization feature to disseminated, aligned plates of graphite. By contrast, the augen gneiss shows low polarizability and a moderately anisotropic conductivity dominated by the pore‐filling brine. We further demonstrate that neglecting the complex and frequency‐dependent nature of conductivity can lead to serious misinterpretation of magnetotelluric data during inversion if highly polarizable rocks are present.
Variations in Gas and Water Pulses at an Arctic Seep: Fluid Sources and Methane Transport
Authors: Hong W.‐L. , Torres M. E. , Portnov A. , Waage M. , Haley B. , Lepland A. 
Published: April 2018

AbstractMethane fluxes into the oceans are largely dependent on the methane phase as it migrates upward through the sediments. Here we document decoupled methane transport by gaseous and aqueous phases in Storfjordrenna (offshore Svalbard) and propose a three‐stage evolution model for active seepage in the region where gas hydrates are present in the shallow subsurface. In a preactive seepage stage, solute diffusion is the primary transport mechanism for methane in the dissolved phase. Fluids containing dissolved methane have high 87Sr/86Sr ratios due to silicate weathering in the microbial methanogenesis zone. During the active seepage stage, migration of gaseous methane results in near‐seafloor gas hydrate formation and vigorous seafloor gas discharge with a thermogenic fingerprint. In the postactive seepage stage, the high concentration of dissolved lithium points to the contribution of a deeper‐sourced aqueous fluid, which we postulate advects upward following cessation of gas discharge.
Changes in the Response of the Northern Hemisphere Carbon Uptake to Temperature Over the Last Three Decades
Authors: Yin Yi , Ciais Philippe , Chevallier Frederic , Li Wei , Bastos Ana , Piao Shilong , Wang Tao , Liu Hongyan 
Published: April 2018

AbstractThe CO2 seasonal cycle amplitude (SCA) in the Northern Hemisphere has increased since the 1960s—a feature attributed mainly to enhanced vegetation activity along climate warming and CO2 increase. We identified a temporal change in the sign of the correlation between SCA and air temperature (T) from positive to negative around the year 2000 at most Northern Hemisphere ground stations, consistent with signals from satellite column CO2 measurements since the mid‐2000s. Further, we explored potential causes of this change using net biome productivity estimates from three atmospheric inversions for the period 1980–2015. The change in the SCA‐T relationship is primarily attributable to changes in the net biome productivity‐T relationship: positive correlations weakened in the spring in the high latitudes, confirming a limit to the “warmer spring‐bigger carbon sink” mechanism; negative correlations diminished in the autumn/winter in the mid‐to‐high latitudes, challenging the “warmer winter‐larger carbon release” assumption and highlighting the complexity of carbon processes outside the peak growing season.
Soil Moisture Sensing Using Spaceborne GNSS Reflections: Comparison of CYGNSS Reflectivity to SMAP Soil Moisture
Authors: Chew C. C. , Small E. E. 
Published: April 2018

AbstractThis paper quantifies the relationship between forward scattered L‐band Global Navigation Satellite System (GNSS) signals, recorded by the Cyclone Global Navigation Satellite System (CYGNSS) constellation and Soil Moisture Active Passive (SMAP) soil moisture (SM). Although designed for tropical ocean surface wind sensing, the CYGNSS receivers also record GNSS reflections over land. The CYGNSS observations of reflection power are compared to SMAP SM between March 2017 and February 2018. A strong, positive linear relationship exists between changes in CYGNSS reflectivity and changes in SMAP SM, but not between the absolute magnitudes of the two observations. The sensitivity of CYGNSS reflectivity to SM varies spatially and can be used to convert reflectivity to estimates of SM. The unbiased root‐mean‐square difference between daily averaged CYGNSS‐derived SM and SMAP SM is 0.045 cm3/cm3 and is similarly low between CYGNSS and in situ SM. These results show that CYGNSS, and future GNSS reflection missions, could provide global SM observations.
Toward personalised diffusion MRI in psychiatry: improved delineation of fibre bundles with the highest-ever angular resolution in vivo tractography
Authors: Callaghan Fraser , Maller Jerome J. , Welton Thomas , Middione Matthew J. , Shankaranarayanan Ajit , Grieve Stuart M. 
Published: April 2018

AbstractDiffusion MRI (dMRI) tractography is a uniquely powerful tool capable of demonstrating structural brain network abnormalities across a range of psychiatric disorders; however, it is not currently clinically useful. This is because limitations on sensitivity effectively restrict its application to scientific studies of cohorts, rather than individual patients. Recent improvements in dMRI hardware, acquisition, processing and analysis techniques may, however, overcome these measurement limitations. We therefore acquired the highest-ever angular resolution in vivo tractographic data set, and used these data to ask the question: 'is cutting-edge, optimised dMRI now sensitive enough to measure brain network abnormalities at a level that may enable personalised psychiatry?' The fibre tracking performance of this 'gold standard' data set of 1150 unique directions (11 shells) was compared to a conventional 64-direction protocol (single shell) and a clinically practical, highly optimised and accelerated 9-min protocol of 140 directions (3 shells). Three major tracts of relevance to psychiatry were evaluated: the cingulate bundle, the uncinate fasciculus and the corticospinal tract. We found up to a 34-fold improvement in tracking accuracy using the 1150-direction data set compared to the 64-direction data set, while 140-direction data offered a maximum 17-fold improvement. We also observed between 20 and 50% improvements in tracking efficiency for the 140-direction data set, a finding we then replicated in a normal cohort (n = 53). We found evidence that lower angular resolution data may introduce systematic anatomical biases. These data highlight the imminent potential of dMRI as a clinically meaningful technique at a personalised level, and should inform current practice in clinical studies.
Helicobacter pylori-induced IL-33 modulates mast cell responses, benefits bacterial growth, and contributes to gastritis
Authors: Lv Yi-pin , Teng Yong-sheng , Mao Fang-yuan , Peng Liu-sheng , Zhang Jin-yu , Cheng Ping , Liu Yu-gang , Kong Hui , Wang Ting-ting , Wu Xiao-long , Hao Chuan-jie , Chen Weisan , Yang Shi-ming , Zhao Yong-liang , Han Bin , Ma Qiang , Zou Quan-ming , Zhuang Yuan 
Published: April 2018

AbstractInterleukin (IL)-induced inflammatory responses are critical for the pathogenesis of Helicobacter pylori (H. pylori)-induced gastritis. IL-33 represents a recently discovered proinflammatory cytokine involved in inflammatory diseases, but its relevance to H. pylori-induced gastritis is unknown. Here, we found that gastric IL-33 mRNA and protein expression were elevated in gastric mucosa of both patients and mice infected with H. pylori, which is positively correlated with bacterial load and the degree of gastritis. IL-33 production was promoted via extracellular regulated protein kinases (ERK) signaling pathway activation by gastric epithelial cells in a cagA-dependent manner during H. pylori infection, and resulted in increased inflammation and bacteria burden within the gastric mucosa. Gastric epithelial cell-derived IL-33 promoted TNF-α production from mast cells in vitro, and IL-33 increased TNF-α production in vivo. Increased TNF-α inhibited gastric epithelial cell proliferation, conducing to the progress of H. pylori-associated gastritis and bacteria colonization. This study defined a patent regulatory networks involving H. pylori, gastric epithelial cell, IL-33, mast cell, and TNF-α, which jointly play a pathological effect within the gastric circumstances. It may be a valuable strategy to restrain this IL-33-dependent pathway in the treatment of H. pylori-associated gastritis.
BAP1 induces cell death via interaction with 14-3-3 in neuroblastoma
Authors: Sime Wondossen , Niu Qiankun , Abassi Yasmin , Masoumi Katarzyna Chmielarska , Zarrizi Reihaneh , Køhler Julie Bonne , Kjellström Sven , Lasorsa Vito Alessandro , Capasso Mario , Fu Haian , Massoumi Ramin 
Published: April 2018

AbstractBRCA1-associated protein 1 (BAP1) is a nuclear deubiquitinating enzyme that is associated with multiprotein complexes that regulate key cellular pathways, including cell cycle, cellular differentiation, cell death, and the DNA damage response. In this study, we found that the reduced expression of BAP1 pro6motes the survival of neuroblastoma cells, and restoring the levels of BAP1 in these cells facilitated a delay in S and G2/M phase of the cell cycle, as well as cell apoptosis. The mechanism that BAP1 induces cell death is mediated via an interaction with 14-3-3 protein. The association between BAP1 and 14-3-3 protein releases the apoptotic inducer protein Bax from 14-3-3 and promotes cell death through the intrinsic apoptosis pathway. Xenograft studies confirmed that the expression of BAP1 reduces tumor growth and progression in vivo by lowering the levels of pro-survival factors such as Bcl-2, which in turn diminish the survival potential of the tumor cells. Patient data analyses confirmed the finding that the high-BAP1 mRNA expression correlates with a better clinical outcome. In summary, our study uncovers a new mechanism for BAP1 in the regulation of cell apoptosis in neuroblastoma cells.
Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s
Authors: Benoit Matthieu P.M.H. , Asenjo Ana B. , Sosa Hernando 
Published: April 2018

AbstractKinesin-13s constitute a distinct group within the kinesin superfamily of motor proteins that promote microtubule depolymerization and lack motile activity. The molecular mechanism by which kinesin-13s depolymerize microtubules and are adapted to perform a seemingly very different activity from other kinesins is still unclear. To address this issue, here we report the near atomic resolution cryo-electron microscopy (cryo-EM) structures of Drosophila melanogaster kinesin-13 KLP10A protein constructs bound to curved or straight tubulin in different nucleotide states. These structures show how nucleotide induced conformational changes near the catalytic site are coupled with movement of the kinesin-13-specific loop-2 to induce tubulin curvature leading to microtubule depolymerization. The data highlight a modular structure that allows similar kinesin core motor-domains to be used for different functions, such as motility or microtubule depolymerization.
Integrative network analyses of wilt transcriptome in chickpea reveal genotype dependent regulatory hubs in immunity and susceptibility
Authors: Ashraf Nasheeman , Basu Swaraj , Narula Kanika , Ghosh Sudip , Tayal Rajul , Gangisetty Nagaraju , Biswas Sushmita , Aggarwal Pooja R. , Chakraborty Niranjan , Chakraborty Subhra 
Published: April 2018

AbstractHost specific resistance and non-host resistance are two plant immune responses to counter pathogen invasion. Gene network organizing principles leading to quantitative differences in resistant and susceptible host during host specific resistance are poorly understood. Vascular wilt caused by root pathogen Fusarium species is complex and governed by host specific resistance in crop plants, including chickpea. Here, we temporally profiled two contrasting chickpea genotypes in disease and immune state to better understand gene expression switches in host specific resistance. Integrative gene-regulatory network elucidated tangible insight into interaction coordinators leading to pathway determination governing distinct (disease or immune) phenotypes. Global network analysis identified five major hubs with 389 co-regulated genes. Functional enrichment revealed immunome containing three subnetworks involving CTI, PTI and ETI and wilt diseasome encompassing four subnetworks highlighting pathogen perception, penetration, colonization and disease establishment. These subnetworks likely represent key components that coordinate various biological processes favouring defence or disease. Furthermore, we identified core 76 disease/immunity related genes through subcellular analysis. Our regularized network with robust statistical assessment captured known and unexpected gene interaction, candidate novel regulators as future biomarkers and first time showed system-wide quantitative architecture corresponding to genotypic characteristics in wilt landscape.
Multi-lectin Affinity Chromatography and Quantitative Proteomic Analysis Reveal Differential Glycoform Levels between Prostate Cancer and Benign Prostatic Hyperplasia Sera
Authors: Totten Sarah M. , Adusumilli Ravali , Kullolli Majlinda , Tanimoto Cheylene , Brooks James D. , Mallick Parag , Pitteri Sharon J. 
Published: April 2018

AbstractCurrently prostate-specific antigen is used for prostate cancer (PCa) screening, however it lacks the necessary specificity for differentiating PCa from other diseases of the prostate such as benign prostatic hyperplasia (BPH), presenting a clinical need to distinguish these cases at the molecular level. Protein glycosylation plays an important role in a number of cellular processes involved in neoplastic progression and is aberrant in PCa. In this study, we systematically interrogate the alterations in the circulating levels of hundreds of serum proteins and their glycoforms in PCa and BPH samples using multi-lectin affinity chromatography and quantitative mass spectrometry-based proteomics. Specific lectins (AAL, PHA-L and PHA-E) were used to target and chromatographically separate core-fucosylated and highly-branched protein glycoforms for analysis, as differential expression of these glycan types have been previously associated with PCa. Global levels of CD5L, CFP, C8A, BST1, and C7 were significantly increased in the PCa samples. Notable glycoform-specific alterations between BPH and PCa were identified among proteins CD163, C4A, and ATRN in the PHA-L/E fraction and among C4BPB and AZGP1 glycoforms in the AAL fraction. Despite these modest differences, substantial similarities in glycoproteomic profiles were observed between PCa and BPH sera.