bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrochemical Monitoring of Saos‐2 Cell Differentiation on Pyrolytic Carbon Electrodes
Authors: Mohamed Hassan Yasmin , Massa Leonardo , Caviglia Claudia , Sylvest Keller Stephan 
Published: September 2018

AbstractIn this study we establish an electrochemical platform based on two dimensional (2D) pyrolytic carbon electrodes for in vitro analysis of osteoblast differentiation. Electrochemical impedance spectroscopy (EIS) was used to monitor cell adhesion and proliferation, while an electrochemical assay based on square wave voltammetry (SWV) was applied to measure the activity of the differentiation marker alkaline phosphatase (ALP). 2D pyrolytic carbon electrodes were fabricated and used to monitor Saos‐2 cell differentiation for a period of up to 21 days. With this method it was possible to detect a faster increase of ALP activity for cells cultured in medium supplemented with differentiation factors compared to cells cultured in growth medium. This was confirmed by the results obtained with Alizarin Red staining, showing that cells subjected to osteogenic medium went through the entire differentiation process, from proliferation to mineralization. Finally, for the first time, real‐time monitoring of ALP activity combined with continuous EIS monitoring of the same cell culture was achieved using the pyrolytic carbon electrodes.
Bismuth Vanadate/Reduced Graphene Oxide Nanocomposite Electrode for Photoelectrochemical Determination of Diclofenac in Urine
Authors: do Prado Thiago Martimiano , Cincotto Fernando Henrique , Fatibello‐Filho Orlando , Cruz de Moraes Fernando 
Published: September 2018

AbstractThe nanocomposite material bismuth vanadate and reduced graphene oxide BiVO4/rGO was prepared and deposited onto a fluorine‐doped tin oxide glass surface for application of a photoelectrochemical sensor for the determination of sodium diclofenac in urine samples. This drug is considered as an emergent pollutant because of its ecotoxicological risk when discarded in wastewater. Electrochemistry Impedance Spectroscopy experiments shown that the rGO contributed to the efficiency of the DCF electro‐oxidization and, under irradiation, the resistance in the charge transfer in the work electrode decreased. The sensor presented photocurrent responses during the diclofenac electro‐oxidation under visible light irradiation, with a linear concentration range from 9.6×10−9 mol L−1 to 9.2×10−6 mol L−1 and a LOD of 4.2×10−9 mol L−1. The presence of the drug in urine was successfully determined by recovery in spiked samples.
Understanding the Behavior of Electrosprayed Carbon Black‐Nafion Composite Layers
Authors: Conde J. J. , Chaparro A. M. , Ferreira‐Aparicio P. 
Published: September 2018

AbstractElectrosprayed composite films prepared from carbon and Nafion are especially suitable to be applied as microporous layers and protective coatings for a number of for proton exchange membrane fuel cell (PEMFC) components, due to of their particular properties. The electrospray deposition of carbon inks is a complex process that requires further study. In order to gain insight into the factors influencing the characteristics of electrosprayed films, the effect of some parameters has been analyzed. The solvent nature, the Nafion to carbon ratio in the ink, the ionization voltage (positive or negative) and the polymer distribution on the carbon surface are some of the studied variables. Surface characterization of these layers by techniques, such as XPS, SEM or thermal decomposition has provided very useful information about the structure of the layers and the interaction between their components. The results show that electrospray ionization induces a strong adsorption of the sulfonic groups of the ionomer on the carbon black surface, which is probably responsible for the high stability and durability of the layers and their superhydrophobic character. To our knowledge, this is the first study devoted to the analysis of the particular carbon‐Nafion interactions in electrosprayed films.
Vertically Aligned Platinum Copper Nanotubes as PEM Fuel Cell Cathode: Elaboration and Fuel Cell Test
Authors: Marconot O. , Pauc N. , Buttard D. , Morin A. 
Published: September 2018

AbstractThis paper describes the elaboration via an electrochemistry route of a PEM carbon‐free cathode made of a periodic array of vertically aligned platinum copper nanotubes. Nanotubes synthesis is assisted with a porous alumina mold made on a silicon substrate to perform growth of copper nanowires. After optimization of an original confined galvanic displacement with H2PtCl6 salt, PtCu nanotubes are embedded into porous alumina template and directly integrated onto a Nafion® membrane. Therefore, our process does not need any step of purification or catalyst dispersion. The performances in fuel cell of these new electrodes are compared to conventional structures made of Tanaka Pt/C with the same electrocatalytic surface area. Significant improvement in the specific activity at 0.9 V and reduction in mass transport limitations are observed compared with standard Tanaka Pt/C catalyst in real operating conditions.
New frontiers in community initiatives to increase vegetable consumption
Authors: Noy Sue , Patrick Rebecca , Henderson‐Wilson Claire , Nuttman Sonia , Ryan Isabella 
Published: September 2018

AbstractIssue addressedPublic health concerns about insufficient consumption of vegetables across all demographics in Australia have led to 20 years of behaviour change interventions ranging from social marketing to interactive small group programs, with modest results. To maximise health promotion intervention outcomes, practitioners need up‐to‐date information that helps them navigate the complexity of food systems and eating behaviours.MethodsThis scoping review of Australian and international research, including peer‐reviewed and grey literature, provides a picture of health promotion nutrition interventions, as well as other initiatives that may promote increased vegetable consumption. Search terms related to nutrition and vegetable consumption, type of intervention or initiative, for example, campaign; and consumer values and behaviour. A wide range of data sources were used including scholarly papers, market research reports and publicly available websites of community organisations (eg, OOOOBY). A broad food systems typology was developed to provide a framework for the review.ResultsThe review finds an emerging group of community‐driven initiatives within local food systems that appear to have positive impacts on vegetable consumption. These initiatives sit within a multi‐faceted approach to health and well‐being that is consistent with the tenets of the Ottawa Charter for Health Promotion, including community engagement, social justice and sustainability goals.ConclusionsMore research into the impact of these new frontiers is needed, but our preliminary findings point to the potential for health promotion practitioners to collaborate on local/community food system initiatives that are not motivated primarily by health goals, but have the potential to deliver multiple health and environmental outcomes.So what?This review demonstrated community‐driven initiatives around local food systems show the most promise in promoting vegetable consumption and addressing the determinants of health. Health promotion efforts to encourage food security and healthy eating could be strengthened through collaborations within these new frontiers.
Health insurance in Myanmar: Knowledge, perceptions, and preferences of Social Security Scheme members and general adult population
Authors: Myint Chaw‐Yin , Pavlova Milena , Groot Wim 
Published: September 2018

SummaryObjectiveOur study explores the knowledge, perceptions, willingness to pay, and preferences of potential health insurance beneficiaries about health insurance in Myanmar.MethodsCross‐sectional survey data were collected among two samples: the general population and Social Security Scheme (SSS) member. Mann‐Whitney U test and independent sample t test were applied to compare the two samples. The data on willingness to pay for health insurance were analyzed using regression analysis.ResultsLow level of knowledge and weak positive perception are found in both samples. More than 90% of the SSS sample and 75% of the general sample are willing to pay health insurance premiums. The largest shares of both samples are willing to pay for monthly premiums between 2000 and 4000 MMK (1.8‐3.6 USD). Health status, age, gender, income, and trust are significantly associated with willingness to pay for health insurance among general sample while occupation, civil status, income, and positive perception on prepayment principle are found among SSS sample.ConclusionsThe government of Myanmar should be aware of the preferences of beneficiaries to pay a relatively low level of monthly health insurance premiums without co‐payment.
Rural‐urban disparities in family physician practice patterns: A nationwide survey in Taiwan
Authors: Cheng Bo‐Ren , Chang Hsiao‐Ting , Lin Ming‐Hwai , Chen Tzeng‐Ji , Chou Li‐Fang , Hwang Shinn‐Jang 
Published: September 2018

SummaryIntroductionIn a world with increasing urbanization, rural‐urban disparities in health care utilization have been a long‐term concern. However, the details regarding the practice patterns of family physicians in Taiwan have not received sufficient attention thus far.MethodsThe National Health Insurance Research Database of Taiwan offered 0.2% of the total ambulatory visit records for Taiwan in 2013. Records from community clinics of family medicine were collected, with the clinics categorized as rural, suburban, or urban area clinics according to their locations.ResultsAmong 100 334 visits to family medicine clinics, the median patient age was 50 years for urban clinics, 51 for suburban clinics, and 58 for rural clinics. The distributions of patient ages differed in the three areas (P < 0.001). Four types of chronic diseases (cardiovascular diseases, diabetes, chronic respiratory diseases, and cancers) accounted for 10.8%, 11.3%, and 13.6%, of the visits to urban, suburban, and rural clinics, respectively. The most common procedure was wound treatment, and the pattern of the top 10 procedures was similar in the three areas.ConclusionAlthough rural patients in Taiwan were older and had more chronic diseases than urban and suburban patients, the pattern of procedures undertaken by rural family physicians did not differ from those of urban and suburban family physicians.
Ideal embryo transfer position and endometrial thickness in IVF embryo transfer treatment
Authors: Wang Yao , Zhu Yanwen , Sun Yun , Di Wen , Qiu Meiting , Kuang Yanping , Shen Haoran 
Published: September 2018

AbstractObjectiveTo establish an ideal transfer strategy by investigating the relationships among embryo transfer (ET) depth, endometrial thickness, and subsequent in vitro fertilization treatment clinical pregnancy outcomes.MethodsIn the present retrospective analysis, data from in vitro fertilization‐ET treatment cycles conducted at a fertility center in Shanghai, China, between October 2014 and March 2015 were analyzed. Women were divided into groups 1–4 according to transfer depth (<10; 10–15, 15–20, and >20 mm, respectively), as measured by air bubbles. Additionally, 391 women were divided into groups A–C according to endometrial thickness (<7, 1–12, and >12 mm, respectively). Clinical pregnancy outcomes were assessed by group.ResultsData from 501 cycles were included. Clinical pregnancy and live delivery rates were significantly higher in group 2 (P=0.009 and P=0.002, respectively) and group 3 (P=0.008 and P=0.001, respectively) than in group 4. Among the 394 patients with endometrial thickness data available, clinical pregnancy and live delivery rates were higher in group B (P=0.028 and P=0.015, respectively) and group (P=0.013 and P=0.013, respectively) than in group A.ConclusionCorrect transfer depth and endometrial thickness can increase the rates of clinical pregnancy, implantation, and live delivery. Placing the embryos at 10–20 mm from the fundus and at an endometrial thickness of more than 7 mm is recommended for optimal clinical pregnancy outcomes.
Bladder urothelial carcinoma producing insulin‐like growth factor II: A case report
Authors: Funada Satoshi , Kita Yuki , Okada Yoshiyuki , Kobayashi Takashi , Teramoto Yuki , Shibuya Shinsuke , Saito Ryoichi , Murakami Kaoru , Matsumoto Keiyu , Yang Feng , Dimitrov Dimiter S , Inoue Takahiro , Ogawa Osamu 
Published: September 2018

IntroductionNon‐islet cell tumor hypoglycemia is a rare paraneoplastic syndrome associated with tumors. Although it mainly occurs in solid tumors of mesenchymal and epithelial origin, but rarely also in hematopoietic and neuroendocrine origin.Case presentationWe describe a 65‐year‐old man with a muscle‐invasive bladder urothelial carcinoma, which rapidly progressed against systemic chemotherapy consisting of gemcitabine and cisplatin. Notably, the patient developed hypoglycemia at the terminal stage of the disease. Pathological diagnosis was giant cell urothelial carcinoma, which was strongly positive for insulin‐like growth factor‐II in immunohistochemistry. We established patient‐derived xenograft from insulin‐like growth factor‐II producing bladder urothelial carcinoma that caused non‐islet cell tumor hypoglycemia. Although we evaluated the efficacy of the neutralizing antibody, there was no statistically significant inhibitory effect on tumor growth.ConclusionTo the best of our knowledge, this is the first report of insulin‐like growth factor‐II‐producing urothelial carcinoma that have been recapitulated in a patient‐derived xenograft model.
Non‐ischemic priapism following recurrent idiopathic ischemic priapism treated successfully with selective arterial embolization and postoperative vacuum therapy before delayed inflatable penile prosthesis placement: A single case report
Authors: Del Giudice Francesco , Busetto Gian Maria , Chung Benjamin I , Conti Simon L , Ferro Matteo , Maggi Martina , Sciarra Alessandro , Perito Paul E , Gross Martin S , Antonini Gabriele , De Berardinis Ettore 
Published: September 2018

IntroductionPriapism is defined as a persistent tumescence or erection of the penis not associated to sexual desire and/or stimulation. Idiopathic recurrent priapism may also occasionally follow treatment of veno‐occlusive priapism and represents a diagnostic and therapeutic challenge.Case presentationWe report a single case of non‐ischemic priapism that resulted after distal shunting procedure for severe and prolonged ischemic priapism and yet occurred without evidence of a cavernosal–sinusoidal fistula that was successfully treated with a bilateral selective arterial embolization. Our protocol of delayed inflatable penile prosthesis placement after a vacuum erectile device program was implemented.ConclusionsThe continuing use of a vacuum erectile device represented a bridge and an adjuvant therapy useful to facilitate later prosthesis placement. Anatomical and functional outcomes were optimal. No postoperative complaints or complications were reported.
Critical realism and ICT4D research
Authors: Heeks Richard , Wall P.J. 
Published: September 2018

AbstractThere is little overt engagement with research paradigms in ICT4D research, but what there is shows a dominance of positivism and interpretivism. In this paper, we explore the value of a “third way” research paradigm: critical realism. We concisely review the main features of critical realism: its ontological realism combined with epistemological relativism; its iterative, pluralist, and reflexive methodology; and its emancipatory values. Alongside the general value of explicit use of any research paradigm, we argue two particular types of value of critical realism for ICT4D research. First, generic values including exposure of context, a contingent causality that reflects real‐world ICT4D experiences, support for use of theoretical frames in ICT4D, legitimisation of different stakeholder views, and reduction of research bias and support for ICT4D's interventionist approach and its goal of delivering international development. Second, specific value in addressing current trends in ICT4D research: The growing search for causal links between “ICT” and “D,” and the political and ethical turns in ICT4D that are spurring researchers to engage more with issues of power, rights, and justice. But delivery of critical realism's utility will require the ICT4D research community to take actions that enable this emergent research paradigm to flourish.
Low‐dose bisphenol A induces RIPK1‐mediated necroptosis in SH‐SY5Y cells: Effects on TNF‐α and acetylcholinesterase
Authors: Ayazgök Beyza , Tüylü Küçükkılınç Tuba 
Published: September 2018

AbstractBisphenol A (BPA) is an endocrine disruptor chemical, which is commonly used in everyday products. Adverse effects of its exposure are reported even at picomolar doses. Effects of picomolar and nanomolar concentrations of BPA on cytotoxicity, nitric oxide (NO) levels, acetylcholinesterase (AChE) gene expression and activity, and tumor necrosis factor‐α (TNF‐α) and caspase‐8 levels were determined in SH‐SY5Y cells. The current study reveals that low‐dose BPA treatment induced cytotoxicity, NO, and caspase‐8 levels in SH‐SY5Y cells. We also evaluated the mechanism underlying BPA‐induced cell death. Ours is the first report that receptor‐interacting serine/threonine‐protein kinase 1–mediated necroptosis is induced by nanomolar BPA treatment in SH‐SY5Y cells. This effect is mediated by altered AChE and decreased TNF‐α levels, which result in an apoptosis‐necroptosis switch. Moreover, our study reveals that BPA is an activator of AChE.
Development and validation of serum exosomal microRNAs as diagnostic and prognostic biomarkers for hepatocellular carcinoma
Authors: Xue Xiaofeng , Zhao Yubin , Wang Xiaona , Qin Lei , Hu Rongkuan 
Published: September 2018

AbstractHepatocellular carcinoma (HCC) is the second leading cause of cancer‐related death worldwide. China accounts for over half of the new cases and deaths. Diagnostic imprecision and a lack of complimentary molecular biomarkers are partially responsible for this lack of progress. Herein, serum‐derived exosomal microRNA (miRNA) profiling was performed on 80 patients which histologically confirmed HCC and 30 normal controls. A classification of 8 exosomal miRNAs had biologically and statistically significant differences between HCC and normal serum samples, including miR‐122, miR‐125b, miR‐145, miR‐192, miR‐194, miR‐29a, miR‐17‐5p, and miR‐106a. Online algorithm showed strong independent classification accuracy (area under the curve) reached 0.535 to 0.850, separately. The significant correlation between serum exosomal miRNAs and tumor size was observed. In addition, the survival difference of HCC patients with high or low exosomal miR‐106a was statistically significant using Kaplan‐Meier analysis. Besides, we also measured the proliferation and invasion ability of HCC cells following exosomal miR‐106a mimics or inhibitor treatment. After prediction with algorithms, mitogen‐activated protein kinase and c‐Jun N‐terminal kinase pathways were identified associated with miR‐106a’s function. In summary, differentially expressed serum exosomal miRNAs can be helpful for diagnostic and prognostic of HCC.
Structural organization of the olfactory organ in an amphihaline migratory fish Hilsa, Tenualosa ilisha
Authors: Malick Chandan , Chatterjee Subhendu Kumar , Bhattacharya Samir , Suresh Vettath Raghavan , Kundu Rakesh , Saikia Surjya Kumar 
Published: September 2018

AbstractThe histological as well as ultramicroscopic structures of olfactory system of an amphihaline migratory fish hilsa Tenualosa ilisha, were studied. The sexually matured riverine fish were collected from a common breeding habitat—the Hooghly, a tributary of river Ganga, West Bengal, India. This study revealed that the riverine hilsa has larger olfactory bulb compared to marine hilsa with the olfactory lobes well exposed through nostrils. The olfactory lamellae (OL) are 40–45 in number and posses three distinct layers of sensory cells across each lamellae, namely, outer receptor cells (RC), middle sensory cells, and inner basal cells (BC). Besides the above arrangement, the sensory part of olfactory epithelium (OE) also bears rich microvillous cells exposed to the surface of the OE. The sensory and non‐sensory surfaces on OL are distinguishable, with clear dendritic cells on sensory epithelium and solitary chemosensory cells on non sensory OE. Abundance of both types of cells in the OE is an indication of its chemoattraction ability towards molecules of amino acid origin. The feature of having abundant, dense, and large dendritic knobs on the surface of OE describes resemblance to the typical morphology of the chemosensory septal organs neuron. The expression of four G protein subunits, like Gαs/olf, Gαq, Gαo, and Gαi‐3 in OE indicate that its olfaction is a functional attributes of two olfactory systems, namely main olfactory system and Vomaronasal Olfactory System. Expression of ACIII and PLCβ2 in OE further confirms two signaling pathways involved in odorant reception in hilsa.Research HighlightsThe olfactory bulb in the amphihaline migratory fish hilsa is big in size, with 40–45 lamellae. Its sensory areas showed multilayered cellular features with prominent sensory as well as microvillous cells, whereas non‐sensory area possesses solitary chemosensory cells. The expression of four G protein subunits, Gαs/olf, Gαq, Gαo, and Gαi‐3 in olfactory epithelium indicates that its olfaction is a functional attributes of two olfactory systems, namely main olfactory system and vomaronasal olfactory system.
Facilitating disclosure in intelligence interviews: The joint influence of helpfulness priming and interpersonal approach
Authors: Neequaye David Amon , Ask Karl , Granhag Pär Anders , Vrij Aldert 
Published: September 2018

AbstractThis study examined the joint influence of helpfulness priming and a helpfulness‐focused interpersonal approach on information disclosure in an intelligence interview. We based the research on the theoretical proposition that consistency between an interviewee's primed dispositions and an interviewer's interpersonal approach would facilitate disclosure. Participants (N = 116) took on the role of an informant with information about an upcoming terror attack. Afterwards, an interviewer solicited information about the attack using an interpersonal approach that exhibited either high (helpfulness‐focused) or low (control) fit with helpfulness concerns. Prior to the interview, in a seemingly unrelated experiment, we primed participants' helpfulness motivation and assessed their cognitive accessibility to helpfulness‐related constructs. We observed that helpfulness priming increased information disclosure when the helpfulness‐focused interpersonal approach was used but not when the control protocol was used. This research suggests that implementation of an interpersonal approach that complements an interviewee's primed dispositions may function symbiotically with the previous priming to facilitate information disclosure.