bluuebunny/crossref_metadata_2025_split huggingface dataset:
7-O-Methylwogonin from Scutellaria baicalensis Disturbs Mitotic Progression by Inhibiting Plk1 Activity in Hep3B Cells
Authors: Woo Sang-Uk , Jang Hay-Ran , Chin Young-Won , Yim Hyungshin 
Published: September 2018

AbstractPolo-like kinase 1, a mitotic Ser/Thr kinase, has emerged as a molecular target for the development of anticancer drugs. In this study, we found that polo-like kinase 1 activity was inhibited by 7-O-methylwogonin and related flavones, including baicalein, dihydrobaicalein, and viscidulin II, isolated from Scutellaria baicalensis. Although dihydrobaicalein exhibited the highest polo-like kinase 1 inhibitory activity among the four compounds, it also inhibited other kinases, such as vaccinia-related kinase 2 and polo-like kinase 2. Baicalein and viscidulin II also showed low selectivity to polo-like kinase 1 since they inhibited polo-like kinase 3 and polo-like kinase 2, respectively. However, 7-O-methylwogonin exhibited selective polo-like kinase 1 inhibitory activity, as evidenced from in vitro kinase assays based on fluorescence resonance energy transfer assays and ADP-Glo kinase assays. In addition, examination of mitotic morphology and immunostaining using specific antibodies for the mitotic markers, p-histone H3 and mitotic protein monoclonal 2, in Hep3B cells showed that 7-O-methylwogonin treatment increased mitotic cell populations due to inhibition of mitotic progression as a result of polo-like kinase 1 inhibition. The pattern of 7-O-methylwogonin-induced mitotic arrest was similar to that of BI 2536, a specific polo-like kinase 1 inhibitor. Thus, it was suggested that 7-O-methylwogonin disturbed mitotic progression by inhibiting polo-like kinase 1 activity. These data suggest that 7-O-methylwogonin, a polo-like kinase 1 inhibitor, may be a useful anticancer agent because of its polo-like kinase 1 selectivity and effectiveness.
Retaining Alkyl Nucleophile Regiofidelity in Transition-Metal-Mediated Cross-Couplings to Aryl Electrophiles
Authors: Cornella Josep , O’Neill Matthew 
Published: September 2018

While the advent of transition-metal catalysis has undoubtedly transformed synthetic chemistry, problems persist with the introduction of secondary and tertiary alkyl nucleophiles into C(sp2) aryl electrophiles. Complications arise from the delicate organometallic intermediates typically invoked by such processes, from which competition between the desired reductive elimination event and the deleterious β-H elimination pathways can lead to undesired isomerization of the incoming nucleophile. Several methods have integrated distinct combinations of metal, ligand, nucleophile, and electrophile to provide solutions to this problem. Despite substantial progress, refinements to current protocols will facilitate the realization of complement reactivity and improved functional group tolerance. These issues have become more pronounced in the context of green chemistry and sustainable catalysis, as well as by the current necessity to develop robust, reliable cross-couplings beyond less explored C(sp2)–C(sp2) constructs. Indeed, the methods discussed herein and the elaborations thereof enable an ‘unlocking’ of accessible topologically enriched chemical space, which is envisioned to influence various domains of application.1	Introduction2	Mechanistic Considerations3	Magnesium Nucleophiles4	Zinc Nucleophiles5	Boron Nucleophiles6	Other Nucleophiles7	Tertiary Nucleophiles8	Reductive Cross-Coupling with in situ Organometallic Formation9	Conclusion
A De Novo Missense Variant in POU3F2 Identified in a Child with Global Developmental Delay
Authors: Riedhammer Korbinian , Kovacs-Nagy Reka , Meitinger Thomas , Hoefele Julia , Wagner Matias , Westphal Dominik 
Published: September 2018

Many genetic and nongenetic causes for developmental delay in childhood could be identified. Often, however, the molecular basis cannot be elucidated. As next-generation sequencing is becoming more frequently available in a diagnostic context, an increasing number of genetic variations are found as causative in children with developmental delay.We performed trio exome sequencing in a girl with developmental delay and minor dysmorphological features. Using a filter for de novo variants, the heterozygous missense variant c.812A>T, p.(Glu217Val) was found in the candidate gene POU3F2 in our patient.
POU3F2 plays an important role in neuronal differentiation and hormonal regulation. To date, it has not been associated with monogenic disorders. Studies on Pou3f2 knockout mice highlighted the importance of this protein in the development of the brain. Furthermore, microdeletions with an overlapping region including only POU3F2 and FBXL4 were linked to developmental delay in six unrelated families. Therefore, POU3F2 is a strong candidate gene for developmental delay, although functional assays proving this assumption still have to be done.
Stand der Therapiestrategien bei älteren Myelom-Patienten
Authors: Schmale Ine 
Published: September 2018

Zusammenfassung
In Turin trafen sich in diesem Jahr zum ersten Mal Ärzte und Wissenschaftler, die sich mit dem multiplen Myelom befassen, auf dem European Myeloma Network Meeting. Im Fokus standen unter anderem der Stellenwert der autologen Stammzelltransplantation und die Behandlung des älteren Patienten mit neu diagnostiziertem multiplen Myelom (NDMM).
Kontinenz und Potenz nach radikaler Prostatektomie
Authors: Schmale Ine 
Published: September 2018

Zusammenfassung
Gibt es einen Zusammenhang zwischen Kontinenz und erektiler Potenz nach radikaler Prostatektomie? Dr. Marius Cristian Butea-Bocu, Kliniken Hartenstein, Bad Wildungen, stellte auf dem Deutschen Krebskongress hierzu Ergebnisse einer Untersuchung vor, welche dieser Frage nachging.
Trends beim Ösophagus- und Magenkarzinom
Authors: Schmale Ine 
Published: September 2018

Zusammenfassung
Die Arbeitsgemeinschaft Deutsche Tumorzentren e. V. greift auf 19 klinische und epidemiologische Register aus 12 Bundesländern zu. In die Auswertung der Jahre 2000 bis 2016 wurden 101 559 Erstdiagnosen für Tumoren des Ösophagus und Magens auf-genommen. Richard Hummel, Lübeck, teilte im Rahmen des Deutschen Krebskongresses Erkenntnisse zur Versorgungsqualität in Deutschland.
Die kardiale Amyloidose – ein Krankheitsbild im Wandel
Authors: Mahrholdt Heiko , Klingel Karin , Sechtem Udo 
Published: September 2018

AbstractA fast and reliable diagnosis of cardiac amyloidosis requires a significant amount of clinical awareness. It is especially important to come to an early diagnosis in patients with cardiac AL amyloidosis in order to improve the otherwise unfavourable clinical course in these patients. There is a significant increase in the number of patients with cardiac amyloidosis of the ATTR wild-type variety. These patients are often elderly males presenting with predominantly right sided heart failure. We present a diagnostic pathway enabling a structured approach to these patients using multimodality cardiac imaging and endomyocardial biopsy. Early chemotherapy is the key to improving symptoms in patients with AL amyloidosis. In contrast, pharmacologic approaches for treating cardiac ATTR amyloidosis need further research and clinical trials.
Chronische Myeloische Leukämie
Authors: Hochhaus Andreas , Eigendorff Ekkehard , Ernst Thomas 
Published: September 2018

AbstractThe advent of tyrosine kinase inhibitors (TKI) has improved prognosis and outcome of patients with chronic myelogenous leukemia (CML) considerably. In comparison to imatinib, first line use of second generation inhibitors nilotinib, dasatinib and bosutinib lead to faster and deeper molecular remissions accompanied by a novel adverse event profile. Essential part of the management of CML patients is a consequent use of cytogenetic and molecular follow up with standardized methods to regularly assess the remission status. Long lasting remission without treatment in an important minority of patients prompted the hope for curability of CML. The use of interferon alpha in parallel with or after TKI therapy is associated with the induction of an immune response against the leukemic clone with further improved remission rate. The cooperative management of CML patients permits the early use of novel treatment options in patients at risk.
Aber klar! Das kann ich! – Selbstbewusstsein
stärken
Authors: Jürgens Ute 
Published: September 2018

ZusammenfassungMit mehr Selbstvertrauen klappt alles besser. Wie kann ich mein inneres Wachstum
und Bewusstsein fördern? Ein positives Selbstbild erleichtert das Leben
beruflich und privat. Weniger Mühsal und mehr Schwung resultieren.
Systematic mapping of free energy landscapes of a growing filamin domain during biosynthesis
Authors: Waudby Christopher A. , Wlodarski Tomasz , Karyadi Maria-Evangelia , Cassaignau Anaïs M. E. , Chan Sammy H. S. , Wentink Anne S. , Schmidt-Engler Julian M. , Camilloni Carlo , Vendruscolo Michele , Cabrita Lisa D. , Christodoulou John 
Published: September 2018

SignificanceEfficient synthesis and folding of proteins, avoiding misfolded states, are central to cell function. As folding may be initiated in parallel with translation, key experimental challenges are to map changes that occur in folding free energy landscapes as translation proceeds and to understand how these landscapes might be modulated by the ribosome and auxiliary factors. Here, we study the length-dependent folding of a domain from a tandem repeat protein and solve the structure of a stable folding intermediate. Although destabilized by the ribosome at equilibrium, modeling of the nonequilibrium folding pathway nevertheless indicates a significant role for proline isomerization during translation. We develop a simple model to explore the impact of cotranslational folding kinetics on misfolding hazards.
Cross-cultural invariances in the architecture of shame
Authors: Sznycer Daniel , Xygalatas Dimitris , Agey Elizabeth , Alami Sarah , An Xiao-Fen , Ananyeva Kristina I. , Atkinson Quentin D. , Broitman Bernardo R. , Conte Thomas J. , Flores Carola , Fukushima Shintaro , Hitokoto Hidefumi , Kharitonov Alexander N. , Onyishi Charity N. , Onyishi Ike E. , Romero Pedro P. , Schrock Joshua M. , Snodgrass J. Josh , Sugiyama Lawrence S. , Takemura Kosuke , Townsend Cathryn , Zhuang Jin-Ying , Aktipis C. Athena , Cronk Lee , Cosmides Leda , Tooby John 
Published: September 2018

SignificanceThis set of experiments shows that in 15 traditional small-scale societies there is an extraordinarily close correspondence between (i) the intensity of shame felt if one exhibited specific acts or traits and (ii) the magnitude of devaluation expressed in response to those acts or traits by local audiences, and even foreign audiences. Three important and widely acknowledged sources of cultural variation between communities—geographic proximity, linguistic similarity, and religious similarity—all failed to account for the strength of between-community correlations in the shame–devaluation link. This supplies a parallel line of evidence that shame is a universal system, part of our species’ cooperative biology, rather than a product of cultural evolution.
Measurement invariance explains the universal law of generalization for psychological perception
Authors: Frank Steven A. 
Published: September 2018

Significance
When an animal is presented with two stimuli, it may consider them similar or different. Similarity often expresses a generalized notion of a category, such as two circles with different sizes, shadings, and colors both being circles. In many studies, perception of similarity declines exponentially with the measure of separation, a pattern often called the universal law of generalization. This article shows that the universal exponential law can be explained by simple properties any reasonable perceptual scale must have. A shift of the scale by a constant amount, or a stretch by a constant amount, should not change the animal’s ability to perceive generalities or differences. Those invariant measurement properties by themselves explain why perceived generalization follows an exponential pattern.
Mapping mammalian replication domains using the ion torrent semiconductor sequencing platform
Authors: Takebayashi Shin-ichiro , Ogata Shin , Ogata Masato , Okumura Katsuzumi 
Published: September 2018

ABSTRACT
Here, we show that semiconductor-based sequencing technology can be used to map mammalian replication domains, chromosomal units with similar DNA replication timing. Replicating DNA purified from mammalian cells was successfully sequenced by the Ion Torrent platform. The resultant replication domain map of mouse embryonic stem cells is comparable to those obtained by the conventional microarray-based method.
At Play in the Republic of Letters: The Correspondence of Lapo da Castiglionchio the Younger
Authors: Kircher Timothy 
Published: September 2018

AbstractThis article examines the letter collection of Lapo da Castiglionchio the Younger (1406–38), an important translator of classical Greek. While scholars have edited the letters chronologically or analyzed them piecemeal, my study shows that as an ensemble the work artfully conveys a cultural and philosophical statement. By playing with time, circumstance, and persona, Lapo reveals the shortcomings of the humanist program, since it associated learning and virtue with public recognition; in addition, the letters elicit the readers’ empathy, as both patronage and Stoic self-reliance are found wanting. The humanist reawakening of the classics was witnessed here in conversation.
Leonardo and the Etruscan Tomb
Authors: Hillard Caroline S. 
Published: September 2018

AbstractLeonardo da Vinci’s drawing of an Etruscan mausoleum has long puzzled scholars. Although they agree that the discovery of an Etruscan tomb at Castellina in Chianti inspired the work, questions remain about the master’s interpretation of Etruscan architecture and its place in his broader oeuvre. Through a reading of early documents related to the tomb discovery, this study offers a new interpretation of the work’s origin, content, and purpose. It situates the work within an epistolary exchange between some of Florence’s leading citizens, and posits that it reflects contemporary views of Etruscan architecture drawn from textual and archaeological sources.
The Role of Research in Economic Development
Authors: Okokpujie I. P. , Fayomi O.S.I. , Leramo R. O. 
Published: September 2018

Abstract
This study channels via the association between analysis utilizations, and financial advancement at the national level in creating country. Innovative works are basic driving advancement, national improvement and financial development, yet there likewise should be objective will to succeed. There must be a solid collaboration amongst the scholarly world and the industries. At the same time, hypothetical, as well as experimental writing has radically demanded that the thing of interests in examine is an irreplaceable part of economic development. The affiliation between inquire about uses and monetary execution has pulled in a lot of consideration in the scholastic field for a long while. Notwithstanding that, the subject has been for some time stressed by governments and private undertakings. The vast majority of the modern financial experts have ascribed the sustained development in developing nations to their escalated inquiresin research works. The dialog of the connection between economic development and research expenses has an uncommon direness, for instance Turkey’s aspiring financial targets. The emphasis on human capital as a driver of economic development for developing nations has prompted undue consideration on school accomplishment. Developing nations have made impressive advance in shutting the hole with regarding school accomplishment, yet ongoing exploration has underscored the significance of subjective aptitudes for economic development. This outcome shifts regard for issues of school quality around the developing nations has been significantly less fruitful in shutting the holes. Without enhancing school quality, developing nations will think that it is hard to enhance their long run financial performance. This paper exposes the impact of research in economic development of developing nations where it is taken in high-regard, shows where research has taken them to, influencing their GNP, NDP, new inventions, established standards.
Elevation of serum creatinine in a renal transplant patient following oral creatine supplementation
Authors: Law Jonathan Philip , Di Gerlando Saveria , Pankhurst Tanya , Kamesh Lavanya 
Published: September 2018

Abstract
We report the case of a renal transplant recipient presenting with elevated serum creatinine levels whilst taking oral creatine ethyl ester (CEE), but not creatine monohydrate (CM). Standard investigations for allograft dysfunction, including Doppler ultrasound and renal biopsy, were normal. Serum creatinine normalized following cessation of the supplement. CM is poorly absorbed and does not affect creatinine. In contrast, CEE is converted and absorbed as creatinine, elevating serum levels. In such cases, creatinine is not a valid surrogate for glomerular filtration rate (GFR). Alternate methods of GFR measurement should be considered and a rigorous clinical and drug history taken.
Impact of modelled PM2.5, NO2 and O3 annual air concentrations on some causes of mortality in Tuscany municipalities
Authors: Uccelli Raffaella , Mastrantonio Marina , Altavista Pierluigi , Pacchierotti Francesca , Piersanti Antonio , Ciancarella Luisella 
Published: September 2018

Abstract

Background
In 2014, the European Environment Agency estimated 59 630 premature deaths in Italy attributable to long-term exposure to PM2.5, 17 290 to NO2 and 2900 to O3. The aim of this study was to test an approach for assessing health impact of the above pollutants analyzing possible associations between annual municipal concentrations, estimated by the national dispersion model developed by ENEA, and mortality rates for trachea, bronchus and lung (TBL) cancer, total respiratory diseases (RD) and chronic obstructive pulmonary diseases (COPD). Tuscany was selected as test case.

Methods
For the 287 municipalities, 2009–13 standardized mortality rates (SMRates) for each cause of death were calculated by the ENEA epidemiological database. The SMRates of municipalities, aggregated on the basis of the 2003 or 2010 estimated pollutant concentration tertiles, were also computed.

Results
TBL cancer SMRate in municipalities with 2003 PM2.5 levels >15.2 μg/m3 was significantly higher than the SMRates of the two lowest tertiles and COPD SMRates in the two highest O3 tertiles were significantly higher than that of the lower tertile. No association between PM2.5 or NO2 concentrations and RD and COPD was detected. Approximately 625 TBL cancer deaths attributable to PM2.5 levels above 10 μg/m3 in 2003 were estimated in the region. Smoking habits and deprivation index were homogeneously distributed among municipalities.

Conclusion
This methodological approach allowed detecting associations between mortality and specific air pollutants even at levels below the Italian normative limits and could be employed to evaluate the potential health impact of air pollution in areas where direct measures of concentration are unavailable.
Structure in a sea of sand: fish abundance in relation to man-made structures in the North Sea
Authors: Wright Serena R , Lynam Christopher P , Righton David A , Metcalfe Julian , Hunter Ewan , Riley Ainsley , Garcia Luz , Posen Paulette , Hyder Kieran 
Published: September 2018

Abstract
Artificial structures in the marine environment may have direct and/or indirect impact on the behaviour and space use of mobile foragers. This study explores whether environmental and physical features in the North Sea—including artificial structures (wrecks, wind turbines, cables, and oil and gas structures) were associated with local abundance of three fish species: cod (Gadus morhua), plaice (Pleuronectes platessa), and thornback ray (Raja clavata). Generalized additive models (GAMs) were used to compare distributions between data collected by fisheries surveys and electronic tags. Distributions of cod, plaice, and ray were correlated with environmental variables including temperature, depth, and substrate, matching findings from previous studies. All species showed seasonal increases in their abundance in areas with high densities of artificial structures, including oil and gas platforms and wrecks. Independent of whether fish purposefully associate with these features or whether structures happen to coincide with locations frequented by these populations, the strong association suggests that greater consideration needs to be given to regulation of habitat alterations, including decommissioning.
Hide and seek in the Bay of Biscay—a functional investigation of marine megafauna and small pelagic fish interactions
Authors: Lambert Charlotte , Authier Matthieu , Doray Mathieu , Dorémus Ghislain , Spitz Jérome , Ridoux Vincent 
Published: September 2018

AbstractPrey and predator distributions influence one another. Understanding the scale and the orientation of predator–prey spatial correlations is crucial in foraging ecology. Growing evidence suggests that predator–prey interactions are more constrained by functional characteristics of both the predator and the prey. Unfortunately, in marine pelagic systems, the scale and orientation of spatial correlations between predators and prey have been only little explored from a functional point of view. We tested the existence of fine-scale association between predators and fish functional groups. Visual predator sightings and acoustic fish records were collected synchronously during oceanographic surveys from 2004 to 2014. Prey biomass was integrated by nautical miles and split into four size classes (&lt;10 cm; 10–20 cm; 20–30 cm; &gt;30 cm) and two depth layers (surface, deep). We computed the relative biomass by prey size and depth category from 0 to 12 nm around predator sightings to determine the predators’ proximity to local prey biomass. Two cetaceans (common, bottlenose dolphins) and three seabirds (northern gannets, auks, northern fulmars) were studied. No association was found in fulmars, indicating they probably do not feed on considered fishes in the area. Gannets and auks were positively correlated with local prey biomass for sizes &lt;20 cm at both depth layers. Significant negative relationships were found between common dolphins and prey size classes &lt;20 cm at both depth layers, and between bottlenose dolphins and all size ranges at the deeper layer. Our results suggest that the fine-scale spatial overlap of predator and prey is influenced by their functional traits, and that prey exhibit predator avoidance behaviour in presence of swimming predators but not of flying ones.