bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spectroscopic Studies of Asparaginyl-tRNA Synthetase from Entamoeba histolytica
Authors: Biswas Priyanka , Sahu Dillip K. , Sahu Kalyanasis , Banerjee Rajat 
Published: July 2019

Background:
Aminoacyl-tRNA synthetases play an important role in catalyzing the first
step in protein synthesis by attaching the appropriate amino acid to its cognate tRNA which then
transported to the growing polypeptide chain. Asparaginyl-tRNA Synthetase (AsnRS) from Brugia
malayi, Leishmania major, Thermus thermophilus, Trypanosoma brucei have been shown to play
an important role in survival and pathogenesis. Entamoeba histolytica (Ehis) is an anaerobic eukaryotic
pathogen that infects the large intestines of humans. It is a major cause of dysentery and has
the potential to cause life-threatening abscesses in the liver and other organs making it the second
leading cause of parasitic death after malaria. Ehis-AsnRS has not been studied in detail, except the
crystal structure determined at 3 Å resolution showing that it is primarily α-helical and dimeric. It is
a homodimer, with each 52 kDa monomer consisting of 451 amino acids. It has a relatively short
N-terminal as compared to its human and yeast counterparts.

Objective:
Our study focusses to understand certain structural characteristics of Ehis-AsnRS using
biophysical tools to decipher the thermodynamics of unfolding and its binding properties.

Methods:
Ehis-AsnRS was cloned and expressed in E. coli BL21DE3 cells. Protein purification
was performed using Ni-NTA affinity chromatography, following which the protein was used for
biophysical studies. Various techniques such as steady-state fluorescence, quenching, circular dichroism,
differential scanning fluorimetry, isothermal calorimetry and fluorescence lifetime studies
were employed for the conformational characterization of Ehis-AsnRS. Protein concentration for
far-UV and near-UV circular dichroism experiments was 8 µM and 20 µM respectively, while 4
µM protein was used for the rest of the experiments.

Results:
The present study revealed that Ehis-AsnRS undergoes unfolding when subjected to increasing
concentration of GdnHCl and the process is reversible. With increasing temperature, it
retains its structural compactness up to 45ºC before it unfolds. Steady-state fluorescence, circular
dichroism and hydrophobic dye binding experiments cumulatively suggest that Ehis-AsnRS undergoes
a two-state transition during unfolding. Shifting of the transition mid-point with increasing
protein concentration further illustrate that dissociation and unfolding processes are coupled indicating
the absence of any detectable folded monomer.

Conclusion:
This article indicates that GdnHCl induced denaturation of Ehis-AsnRS is a two –
state process and does not involve any intermediate; unfolding occurs directly from native dimer to
unfolded monomer. The solvent exposure of the tryptophan residues is biphasic, indicating selective
quenching. Ehis-AsnRS also exhibits a structural as well as functional stability over a wide
range of pH.
Characteristics and Function of the Chitin Binding Protein from Xenorhabdus nematophila
Authors: Liu Jia , Song Ping , Zhang Jie , Nangong Ziyan , Liu Xiaobei , Gao Yue , Wang Qinying 
Published: July 2019

Background:
Genome sequence analysis (GenBank access No.: FN667742.1) shows that
Xenorhabdus nematophila ATCC19061 contains one gene (Xn-cbp) encoding chitin binding protein
(Xn-CBP).

Objective:
The present work aims to clarify the characteristics and function of Xn-CBP from
X. nematophila HB310.

Methods:
In this study, the Xn-cbp gene was cloned and expressed in Escherichia coli BL21 (DE3).
Substrate binding assays were performed to explain the ability of Xn-CBP combined with the polysaccharide.
The insecticidal toxicity of Xn-CBP against the second-instar larvae of Helicoverpa
armigera was determined by feeding method. Besides, the antifungal activity of Xn-CBP against
Coniothyrium diplodiella, Verticillium dahlia, and Fusarium oxysporum was tested by spore germination
assay and hyphal extension assay.

Results:
Xn-CBP encoded 199 amino acids with a calculated mass of 28 kDa, which contained a
signal peptide and a chitin binding domain. The Bmax and Kd values of Xn-CBP to colloidal chitin
were 2.46 and 4.08, respectively. Xn-CBP had insecticidal activity against the H. armigera with a
growth inhibition rate of 84.08%. Xn-CBP had the highest spore germination inhibitory effect on C.
diplodiella with the inhibition rate of 83.11%. The hyphal growth inhibition rate of Xn-CBP to F.
oxysporum, 41.52%, was higher than the other two fungi.

Conclusion:
The Xn-CBP had the highest binding ability to colloidal chitin and it showed insecticidal
activity and antifungal activity. The present study laid a foundation for further exploitation
and utilization of X. nematophila.
Natural Products Derived from the Mediterranean Diet with Antidiabetic Activity: from Insulin Mimetic Hypoglycemic to Nutriepigenetic Modulator Compounds
Authors: Deligiannidou Georgia-Eirini , Philippou Elena , Vidakovic Melita , Berghe Wim V. , Heraclides Alexandros , Grdovic Nevena , Mihailovic Mirjana , Kontogiorgis Christos 
Published: July 2019

Background:
The Mediterranean diet is a healthy eating pattern that protects against the development
of Type 2 diabetes mellitus (T2DM), a metabolic disease characterized by elevated blood sugar levels due to
pancreatic beta-cell functional impairment and insulin resistance in various tissues. Inspired by the ancient communities,
this diet emphasizes eating primarily plant-based foods, including vegetables, legumes, fruits, cereals,
and nuts. Importantly, virgin olive oil is used as the principal source of fat. Red meat is consumed in low amounts
while wine and fish are consumed moderately.

Objective:
Here, we review the most beneficial components of the Mediterranean Diet and tentative mechanisms
of action for prevention and/or management of T2DM, based on research conducted within the last decade.

Methods:
The references over the last five years have been reviewed and they have been selected properly according
to inclusion/ exclusion criteria.

Results:
Several bioactive diet components were evaluated to prevent inflammation and cytokine-induced oxidative
damage, reduce glucose concentration, carbohydrate absorption and increase insulin sensitivity and related
gene expression.

Conclusion:
The adherence to a healthy lifestyle, including diet, exercise and habits remains the best approach
for the prevention of diabetes as well as frequent check-ups and education. Though diabetes has a strong genetic
component, in recent years many reports strongly point to the critical role of lifestyle specific epigenetic modifications
in the development of T2DM. It remains to be established how different components of the Mediterranean
Diet interact and influence the epigenetic landscape to prevent or treat the disease.
Profile of Fatty Acids, Tocopherols, Phytosterols and Polyphenols in Mediterranean Oils (Argan Oils, Olive Oils, Milk Thistle Seed Oils and Nigella Seed Oil) and Evaluation of their Antioxidant and Cytoprotective Activities
Authors: Zarrouk Amira , Martine Lucy , Grégoire Stéphane , Nury Thomas , Meddeb Wiem , Camus Emmanuelle , Badreddine Asmaa , Durand Philippe , Namsi Amira , Yammine Aline , Nasser Boubker , Mejri Mondher , Bretillon Lionel , Mackrill John J. , Cherkaoui-Malki Mustapha , Hammami Mohamed , Lizard Gérard 
Published: July 2019

Background:
The effects of vegetable oils on human health depend on their components. Therefore,
their profiles of lipid nutrients and polyphenols were determined.

Objective:
To establish and compare the fatty acid, tocopherol, phytosterol and polyphenol profiles of Mediterranean
oils: cosmetic and dietary argan oils (AO; Morocco: Agadir, Berkane); olive oils (OO; Morocco, Spain,
Tunisia); milk thistle seed oils (MTSO; Tunisia: Bizerte, Sousse, Zaghouane); nigella seed oil (NSO).

Methods:
The biochemical profiles were determined by gas chromatography-flame ionization, high performance
liquid chromatography and gas chromatography, coupled with mass spectrometry as required. The antioxidant
and cytoprotective activities were evaluated with the KRL (Kit Radicaux Libres) and the fluorescein diacetate
tests on nerve cells treated with 7-ketocholesterol (7KC).

Results:
The fatty acid profile revealed high linoleic acid (C18:2 n-6) content in AO, OO, MTSO and NSO. The
highest levels of oleic acid (C18:1 n-9) were found in AO and OO. The tocopherol profile showed that Agadir
AO contained the highest amount of α-tocopherol, also present at high level in MTSO and Tunisian OO; Berkane
AO was rich in γ-tocopherol. The phytosterol profile indicated that β-sitosterol was predominant in the oils, except
AO; spinasterol was only present in AO. Polyphenol profiles underlined that OO was the richest in polyphenols;
hydroxytyrosol was only found in OO; few polyphenols were detected in AO. The oils studied have antioxidant
activities, and all of them, except NSO, prevented 7KC-induced cell death. The antioxidant characteristics of
AO were positively correlated with procatechic acid and compestanol levels.

Conclusion:
Based on their biochemical profiles, antioxidant and cytoprotective characteristics, AO, OO, and
MTSO are potentially beneficial to human health.
Evaluasi Pembelajaran Mata Kuliah Menejemen Usaha Busana Modiste
Authors: Sintawati Esin , Nurdiansyah Rudi , Purwaningsih Nur Endah 
Published: July 2019

Menejemen Usaha Busana (MUB) Modiste merupakan mata kuliah yang diselenggarakan Program Studi Tata Busana D3 maupun S1.Tujuan penelitian untuk (1) mengetahui pelaksanaan model pembelajaran pada MUB Modiste berkaitan dengan komponen konteks, (2) mengetahui efektivitas model pembelajaran Teaching Factory- enam Model pada tahap input yang berhubungan dengan teknis pengelolaan, administrasi, alokasi waktu serta sarana dan prasarana. Penelitian ini merupakan penelitian evaluasi yang bertujuan untuk mengetahui tingkatketerlaksanaan suatu kebijakan secaracermat dengan cara mengetahui efektivitasmasing-masing komponen yang dievaluasidengan pendekatan model CIPP (Context, Input, Process, Product). Hasil penelitian menunjukkan bahwa (1) pembelajaran MUB Modiste terlaksana karena telah memiliki landasan yuridis berupa kurikulum, sesuai dengan visi misi program studi, (2) pembelajaran MUB Modiste didukung oleh aspek teknis pelaksanaan yang rinci dan konsisten, alokasi waktu pelaksanaan yang memadai untuk pelaksanaan pembelajaran usaha, administrasi yang standar sesuai yang berlaku serta sarana prasarana yang memadai, (3) aspek proses pelaksanaan pembelajaran MUB Modiste telah didukung oleh kompetensi dosen yang sesuai dan memadai, serta dalam prosesnya memiliki model yang sesuai dengan standar dan tujuan pembelajaran.
Penerapan Kesehatan dan Keselamatan Kerja (K3) pada Laboratorium Tata Rias dan Kecantikan
Authors: Tritanti Asi , Siregar Ika Pranita 
Published: July 2019

Penelitian bertujuan: (1) mengetahui penerapan K3 di prodi tata rias untuk diri sendiri, alat dan bahan praktik, serta area kerja, (2) membuat Standar Operasional Prosedur (SOP) yang sesuai K3 untuk kegiatan praktik (2) tata kecantikan kulit, (3) tata kecantikan rambut, dan (4) tata rias pengantin. Penelitian ini adalah penelitian deskriptif kualitatif model interaktif Miles dan Huberman. Subjek penelitian adalah mahasiswa prodi tata rias semester genap 2015/2016 dan semester ganjil 2016/2017, dosen dan laboran Prodi Tata Rias. Data dikumpulkan dengan lembar observasi, kuisioner dan wawancara. Teknik analisis data yang digunakan adalah deskriptif kualitatif. . Hasil penelitian menunjukkan: (1) Kesehatan dan Keselamatan Kerja (K3) di prodi tata rias pada diri sendiri, alat dan bahan praktik, serta area kerja praktik sudah diterapkan. Mahasiswa, laboran dan dosen pengampu memiliki komitmen melakukan K3, memiliki pengetahuan dasar K3, dan bersedia menerapkan K3 sesuai dengan SOP yang berlaku, Standar Operasional Prosedur yang sesuai prinsip K3 untuk (2) bidang keahlian tata kecantikan kulit dibuat dengan memperhatikan prinsip dasar dan kompetensi tata kecantikan kulit, (3) tata kecantikan rambut dibuat dengan memperhatikan prinsip dasar dan kompetensi tata kecantikan rambut, dan (4) praktik tata rias pengantin dibuat dengan memperhatikan konsep dasar dan jenis tata rias pengantin dengan kompetensi rias pengantin Indonesia, pengantin Barat, dan Pengantin Muslim
Pembuatan Tempe Bekatul Dan Kandungan Gizinya
Authors: Auliana Rizqie 
Published: July 2019

Penelitian bertujuan: 1) mengetahui formula tempe bekatul yang tepat sesuai dengan karakteristik bekatul, 2) mengetahui daya terima konsumen terhadap tempe bekatul, dan 3) mengetahui kandungan gizi tempe bekatul berdasarkan analisis proksimat. Jenis penelitian adalah eksperimen di Laboratorium Program Studi Pendidikan Teknik Boga, Universitas Negeri Yogyakarta dan Chemix Pratama. Disain penelitian adalah rancangan blok lengkap 3 kali ulangan percobaan dan 2 kali sampel. Formula tempe bekatul 1 dan 2 menggunakan 7½ % , 10%, dan 12½ %. Hasil penelitian: 1) Formula tempe bekatul yang tepat baik tempe bekatul 1 maupun 2 adalah 10% bekatul, 90% kedelai dan 8% ragi tempe. 2) Tempe bekatul yang disukai adalah formula 2 dengan substitusi 10%. 3) Kandungan gizi tempe bekatul 1 dan 2 tidak berbeda jauh dengan tempe kedelai. Kadar air tempe bekatul 60-61%. Kadar abu tertinggi adalah tempe bekatul 2 sebesar 1,5452%. Kandungan protein tempe kedelai adalah 17,7712%, tempe bekatul 1 adalah 16,1638%, dan tempe bekatul 2 adalah 17,8622%. Kandungan lemak tertinggi dimiliki tempe bekatul 1 sebesar 10,3032% . Serat kasar tempe bekatul 2 lebih rendah 5,4824% dibandingkan tempe bekatul 1. Kandungan karbohidrat dan energi lebih tinggi pada tempe bekatul 1 sebesar 4,7637%
Rancangan Pembelajaran Teaching Factory Di Smk Tata Busana
Authors: Fitrihana Noor 
Published: July 2019

Peraturan Pemerintah Republik Indonesia Nomor 41 Tahun 2015 tentang pembangunan sumber daya industri menyatakan bahwa penyelenggaraan pendidikan vokasi industri berbasis kompetensi harus dilengkapi dengan Lembaga Sertifikasi Profesi (LSP), pabrik dalam sekolah (teaching factory), dan tempat uji kompetensi (TUK). Peraturan Menteri Pendidikan dan Kebudayaan Nomor 22 tahun 2016 tentang standar proses pendidikan dasar dan menengah menyatakan bahwa salah satu model pembelajaran yang perlu dikembangkan adalah pembelajaran yang menghasilkan karya berbasis pemecahan masalah (project based learning) disesuaikan dengan karakteristik kompetensi dan jenjang pendidikan. Kedua peraturan ini menjadi landasan implementasi teaching factory (TEFA) di sekolah menengah kejuruan (SMK). Makalah ini selanjutnya akan membahas tentang rancangan pembelajaran TEFA di SMK kompetensi keahlian Tata Busana
Predicting Dropouts From an Electronic Health Platform for Lifestyle Interventions: Analysis of Methods and Predictors
Authors: Pedersen Daniel Hansen , Mansourvar Marjan , Sortsø Camilla , Schmidt Thomas 
Published: July 2019

Background
The increasing prevalence and economic impact of chronic diseases challenge health care systems globally. Digital solutions can potentially improve efficiency and quality of care, but these initiatives struggle with nonusage attrition. Machine learning methods have been proven to predict dropouts in other settings but lack implementation in health care.

Objective
This study aimed to gain insight into the causes of attrition for patients in an electronic health (eHealth) intervention for chronic lifestyle diseases and evaluate if attrition can be predicted and consequently prevented. We aimed to build predictive models that can identify patients in a digital lifestyle intervention at high risk of dropout by analyzing several predictor variables applied in different models and to further assess the possibilities and impact of implementing such models into an eHealth platform.

Methods
Data from 2684 patients using an eHealth platform were iteratively analyzed using logistic regression, decision trees, and random forest models. The dataset was split into a 79.99% (2147/2684) training and cross-validation set and a 20.0% (537/2684) holdout test set. Trends in activity patterns were analyzed to assess engagement over time. Development and implementation were performed iteratively with health coaches.

Results
Patients in the test dataset were classified as dropouts with an 89% precision using a random forest model and 11 predictor variables. The most significant predictors were the provider of the intervention, 2 weeks inactivity, and the number of advices received from the health coach. Engagement in the platform dropped significantly leading up to the time of dropout.

Conclusions
Dropouts from eHealth lifestyle interventions can be predicted using various data mining methods. This can support health coaches in preventing attrition by receiving proactive warnings. The best performing predictive model was found to be the random forest.
Rapid Creation of an Online Discussion Space (r/nipah) During a Serious Disease Outbreak: Observational Study
Authors: Cole Jennifer , Ezziane Saphia , Watkins Chris 
Published: July 2019

Background
During health emergencies, the people within affected communities ask many questions at a time when professional medics and health agencies are overstretched and struggling to cope. Our previous research has shown that, during the 2014-2015 West Africa Ebola crisis, volunteer-moderated online discussion forums were able to provide peer-to-peer reliable, trustworthy, and well-managed information. We speculated that with the right mix of epistemic and experiential knowledge, such a discussion forum could be set up rapidly during a future serious disease outbreak.

Objective
The aim of this study was to set up a peer-to-peer health information exchange forum within the shortest time possible after the emergence of a real outbreak of a serious infectious disease. An outbreak of Nipah virus in Kerala, India, in May 2018 provided the opportunity to test our theories.

Methods
We initiated a Nipah virus discussion forum on the platform Reddit, recruiting volunteer moderators from within the existing Reddit community. This facilitated posts and comments to the forum from genuine Reddit users. We gathered and analyzed data on the number of posts, comments, page views, and subscribers during the period of May 24 to June 23, 2018, by using the data analysis tools embedded in the Reddit platform.

Results
We were able to set up a functioning health information exchange platform by May 24, 2018, within two weeks of the index case and one week of the official World Health Organization verification of a Nipah virus outbreak. Over the following five weeks, the forum received a steady flow of traffic including posts (36) and comments (21) submitted, page views (840), and subscribers (33). On the busiest day, 368 page views were recorded. The forum provided information in the languages spoken in the outbreak region as well as in English on how the virus spreads, symptoms of the disease, and how to take measures to avoid contracting it. Information on government helpline numbers and frequently asked questions was also provided to the community at risk.

Conclusions
The delivery of a fully functional discussion forum within a short space of time during an actual health emergency demonstrates that our suggestion is fully practical. Our theory that Reddit could provide a suitable platform to host such a forum was upheld. This offers great potential for public health communication during future serious disease outbreaks.
A Mobile Phone App Featuring Cue Exposure Therapy As Aftercare for Alcohol Use Disorders: An Investigator-Blinded Randomized Controlled Trial
Authors: Mellentin Angelina Isabella , Nielsen Bent , Nielsen Anette Søgaard , Yu Fei , Mejldal Anna , Nielsen Dorthe Grüner , Stenager Elsebeth 
Published: July 2019

Background
Cue exposure therapy (CET) is a psychological approach developed to prepare individuals with alcohol use disorder (AUD) for confronting alcohol and associated stimuli in real life. CET has shown promise when treating AUD in group sessions, but it is unknown whether progressing from group sessions to using a mobile phone app is an effective delivery pathway.

Objective
The objectives of this study were to investigate (1) whether CET as aftercare would increase the effectiveness of primary treatment with cognitive behavior therapy, and (2) whether CET delivered through a mobile phone app would be similarly effective to CET via group sessions.

Methods
A total of 164 individuals with AUD were randomized to one of three groups: CET as group aftercare (CET group), CET as fully automated mobile phone app aftercare (CET app), or aftercare as usual. Study outcomes were assessed face-to-face at preaftercare, postaftercare, and again at 6 months after aftercare treatment. Generalized mixed models were used to compare the trajectories of the groups over time on drinking, cravings, and use of urge-specific coping skills (USCS).

Results
In all, 153 of 164 individuals (93%) completed assessments both at posttreatment and 6-month follow-up assessments. No differences in the trajectories of predicted means were found between the experimental groups (CET group and app) compared with aftercare as usual on drinking and craving outcomes over time. Both CET group (predicted mean difference 5.99, SE 2.59, z=2.31, P=.02) and the CET app (predicted mean difference 4.90, SE 2.26, z=2.31, P=.02) showed increased use of USCS compared to aftercare as usual at posttreatment, but this effect was reduced at the 6-month follow-up. No differences were detected between the two experimental CET groups on any outcomes.

Conclusions
CET with USCS delivered as aftercare either via group sessions or a mobile phone app did not increase the effectiveness of primary treatment. This suggests that CET with USCS may not be an effective psychological approach for the aftercare of individuals treated for AUD.

Trial Registration
ClinicalTrials.gov NCT02298751; https://clinicaltrials.gov/ct2/show/NCT02298751