bluuebunny/crossref_metadata_2025_split huggingface dataset:
Static magnetic stimulation of the primary motor cortex impairs online but not offline motor sequence learning
Authors: Lacroix Angélina , Proulx-Bégin Léa , Hamel Raphaël , De Beaumont Louis , Bernier Pierre-Michel , Lepage Jean-François 
Published: July 2019

AbstractStatic magnetic fields (SMFs) are known to alter neural activity, but evidence of their ability to modify learning-related neuroplasticity is lacking. The present study tested the hypothesis that application of static magnetic stimulation (SMS), an SMF applied transcranially via a neodymium magnet, over the primary motor cortex (M1) would alter learning of a serial reaction time task (SRTT). Thirty-nine participants took part in two experimental sessions separated by 24 h where they had to learn the SRTT with their right hand. During the first session, two groups received SMS either over contralateral (i.e., left) or ipsilateral (i.e., right) M1 while a third group received sham stimulation. SMS was not applied during the second session. Results of the first session showed that application of SMS over contralateral M1 impaired online learning as compared to both ipsilateral and sham groups, which did not differ. Results further revealed that application of SMS did not impair offline learning or relearning. Overall, these results are in line with those obtained using other neuromodulatory techniques believed to reduce cortical excitability in the context of motor learning and suggest that the ability of SMS to alter learning-related neuroplasticity is temporally circumscribed to the duration of its application.
Enhanced production of sulforaphane by exogenous glucoraphanin hydrolysis catalyzed by myrosinase extracted from Chinese flowering cabbage (Brassica rapa var. parachinensis)
Authors: Sangkret Supakarn , Pongmalai Patsaporn , Devahastin Sakamon , Chiewchan Naphaporn 
Published: July 2019

AbstractSulforaphane formation via endogenous route is known to be less effective. Exogenous hydrolysis of the sulforaphane precursor is therefore of interest. Here, myrosinase activity was first determined to identify a suitable source of the enzyme from selectedBrassicavegetables. Extracted enzyme was then evaluated for its thermal stability to establish a condition for extraction. Chinese flowering cabbage was selected as the source of myrosinase; suitable extraction condition was at 40 °C for 90 min. Enzyme extract was used to hydrolyze glucoraphanin standard into sulforaphane at 30 °C and pH 6. Exogenous hydrolysis reached the equilibrium with the reverse reaction after 30 min; sulforaphane concentration remained unchanged afterward. Molar fractional conversion of glucoraphanin into sulforaphane at 30-min hydrolysis was around 48%. In comparison with exogenous hydrolysis by myrosinase extracted from broccoli, which indeed exhibits higher activity than the enzyme extracted from Chinese flowering cabbage, no conversion of glucoraphanin into sulforaphane was unexpectedly observed.
Whole genome sequencing of amplified Plasmodium knowlesi DNA from unprocessed blood reveals genetic exchange events between Malaysian Peninsular and Borneo subpopulations
Authors: Benavente Ernest Diez , Gomes Ana Rita , De Silva Jeremy Ryan , Grigg Matthew , Walker Harriet , Barber Bridget E. , William Timothy , Yeo Tsin Wen , de Sessions Paola Florez , Ramaprasad Abhinay , Ibrahim Amy , Charleston James , Hibberd Martin L. , Pain Arnab , Moon Robert W. , Auburn Sarah , Ling Lau Yee , Anstey Nicholas M. , Clark Taane G. , Campino Susana 
Published: July 2019

AbstractThe zoonotic Plasmodium knowlesi parasite is the most common cause of human malaria in Malaysia. Genetic analysis has shown that the parasites are divided into three subpopulations according to their geographic origin (Peninsular or Borneo) and, in Borneo, their macaque host (Macaca fascicularis or M. nemestrina). Whilst evidence suggests that genetic exchange events have occurred between the two Borneo subpopulations, the picture is unclear in less studied Peninsular strains. One difficulty is that P. knowlesi infected individuals tend to present with low parasitaemia leading to samples with insufficient DNA for whole genome sequencing. Here, using a parasite selective whole genome amplification approach on unprocessed blood samples, we were able to analyse recent genomes sourced from both Peninsular Malaysia and Borneo. The analysis provides evidence that recombination events are present in the Peninsular Malaysia parasite subpopulation, which have acquired fragments of the M. nemestrina associated subpopulation genotype, including the DBPβ and NBPXa erythrocyte invasion genes. The NBPXb invasion gene has also been exchanged within the macaque host-associated subpopulations of Malaysian Borneo. Our work provides strong evidence that exchange events are far more ubiquitous than expected and should be taken into consideration when studying the highly complex P. knowlesi population structure.
Tissue amino acid profiles are characteristic of tumor type, malignant phenotype, and tumor progression in pancreatic tumors
Authors: Hiraoka Nobuyoshi , Toue Sakino , Okamoto Chisato , Kikuchi Shinya , Ino Yoshinori , Yamazaki-Itoh Rie , Esaki Minoru , Nara Satoshi , Kishi Yoji , Imaizumi Akira , Ono Nobukazu , Shimada Kazuaki 
Published: July 2019

AbstractTissue amino acid profiles depend on the cell types and extracellular components that constitute the tissue, and their functions and activities. We aimed to characterize the tissue amino acid profiles in several types of pancreatic tumors and lesions. We examined tissue amino acid profiles in 311 patients with pancreatic tumors or lesions. We used newly developed LC-MS/MS methods to obtain the profiles, which were compared with clinicopathological data. Each tumor or lesion presented a characteristic tissue amino acid profile. Certain amino acids were markedly altered during the multistep pancreatic carcinogenesis and pancreatic ductal adenocarcinoma (PDAC) progression. A tissue amino acid index (TAAI) was developed based on the amino acids that were notably changed during both carcinogenesis and cancer progression. Univariate and multivariate survival analyses revealed that PDAC patients with a high TAAI exhibited a significantly shorter survival rate, and these findings were validated using a second cohort. We suggest that tissue amino acid profiles are characteristic for normal tissue type, tumor histological type, and pathological lesion, and are representative of the cancer grade or progression stage in multistep carcinogenesis and of malignant characteristics. The TAAI could serve as an independent prognosticator for patients with PDAC.
Co-expression of key gene modules and pathways of human breast cancer cell lines
Authors: Wu Yadong , liu Feng , Luo Siyang , Yin Xinhai , He Dengqi , Liu Jianguo , Yue Zhaohui , Song Jukun 
Published: July 2019

Abstract
Breast cancer (BC) is the most common leading cause of cancer-related death in women worldwide. Gene expression profiling analysis for human BCs has been studied previously. However, co-expression analysis for BC cell lines is still devoid to date. The aim of the study was to identify key pathways and hub genes that may serve as a biomarker for BC and uncover potential molecular mechanism using weighted correlation network analysis. We analyzed microarray data of BC cell lines (GSE 48213) listed in the Gene Expression Omnibus database. Gene co-expression networks were used to construct and explore the biological function in hub modules using the weighted correlation network analysis algorithm method. Meanwhile, Gene ontology and KEGG pathway analysis were performed using Cytoscape plug-in ClueGo. The network of the key module was also constructed using Cytoscape. A total of 5000 genes were selected, 28 modules of co-expressed genes were identified from the gene co–expression network, one of which was found to be significantly associated with a subtype of BC lines. Functional enrichment analysis revealed that the brown module was mainly involved in the pathway of the autophagy, spliceosome, and mitophagy, the black module was mainly enriched in the pathway of colorectal cancer and pancreatic cancer, and genes in midnightblue module played critical roles in ribosome and regulation of lipolysis in adipocytes pathway. Three hub genes CBR3, SF3B6, and RHPN1 may play an important role in the development and malignancy of the disease. The findings of the present study could improve our understanding of the molecular pathogenesis of breast cancer.
Tension induces intervertebral disc degeneration via endoplasmic reticulum stress-mediated autophagy
Authors: Chen Jiangwei , Lin Zunwen , Deng Kui , Shao Bin , Yang Dong 
Published: July 2019

Abstract
Background: Intervertebral disc degeneration is a common degenerative disease. The present study aimed to explore the role and mechanism of tension-induced endoplasmic reticulum stress in intervertebral disc degeneration.
Methods: Intervertebral disc degeneration models of SD rat were analyzed for apoptosis, the expression of Poly(ADP-ribose) polymerase (PARP), Caspase-12, Caspase-3, LC3, Beclin-1 and CHOP using immunohistochemistry, qPCR and Western blot analysis. Annulus fibrosus cells of intervertebral disc were isolated, subjected to cyclic deformation stress and analyzed for ROS and apoptosis, lysosome activity and expression of genes. The cells were knockdown with siRNA or treated with endoplasmic reticulum stress inhibitor 4-PBA and assayed for ROS, apoptosis, lysosome activity and gene expression.
Results: Compared with the controls, intervertebral disc degeneration was observed through X-rays examinations and HS staining. Apoptosis and expression of PARP, Caspase-12, Caspase-3, LC3, Beclin-1 and CHOP were significantly increased in the intervertebral disc tissue of the models. In mechanic mimic experiments, the primary annulus fibrosus cells were subjected to 18% cyclic deformation, ROS and apoptosis as well as the activity of lysosome were increased. Similarly, the expression of PARP, Caspase-12, Caspase-3, LC3, Beclin-1 and CHOP was also increased significantly after deformation treatment. On other hand, when the cells were treated with 9 mM 4-PBA and/or CHOP-siRNA4, the apoptosis rate, ROS level, lysosome activity and expression of PARP, Caspase-12, Caspase-3, LC3, Beclin-1 and CHOP were significantly reduced.
Conclusions: Autophagy reaction mediated by endoplasmic reticulum stress plays important rale in tension-induced intervertebral disc degeneration. Intervertebral disc degeneration likely results from interactions between autophagy, apoptosis and reticulum stress, and is ROS-dependent.
Safety and efficacy of digital single-operator pancreatoscopy for obstructing pancreatic ductal stones
Authors: Brewer Gutierrez Olaya I. , Raijman Isaac , Shah Raj J. , Elmunzer B. Joseph , Webster George J.M. , Pleskow Douglas , Sherman Stuart , Sturgess Richard P. , Sejpal Divyesh V. , Ko Christopher , Maurano Attilio , Adler Douglas G. , Mullady Daniel K. , Strand Daniel S. , DiMaio Christopher J. , Piraka Cyrus , Sharahia Reem , Dbouk Mohamad H. , Han Samuel , Spiceland Clayton M. , Bekkali Noor L.H. , Gabr Moamen , Bick Benjamin , Dwyer Laura K. , Han Dennis , Buxbaum James , Zulli Claudio , Cosgrove Natalie , Wang Andrew Y. , Carr-Locke David , Kerdsirichairat Tossapol , Aridi Hanaa Dakour , Moran Robert , Shah Shawn , Yang Juliana , Sanaei Omid , Parsa Nasim , Kumbhari Vivek , Singh Vikesh K. , Khashab Mouen A. 
Published: July 2019

Abstract
Background and study aims The role of the digital single-operator pancreatoscopy (D-SOP) with electrohydraulic (EHL) or laser lithotripsy (LL) in treating pancreatic ductal stones is unclear. We investigated the safety and efficacy of D-SOP with EHL or LL in patients with obstructing pancreatic duct stones.
Patients and methods Retrospective analysis of 109 patients who underwent D-SOP for pancreatic stones at 17 tertiary centers in the United States and Europe from February 2015 to September 2017. Logistic regression was performed to identify factors associated with the need for more than one D-SOP with EHL/LL.
Results Most patients were males (70.6 %),mean age 54.7 years. Fifty-nine (54.1 %) underwent EHL and 50 (45.9 %) underwent LL. Mean procedure time was longer in the EHL group (74.4 min vs 53.8 min; P < 0.001). Ducts were completely cleared (technical success) in 89.9 % of patients (94.1 % in EHL vs 100 % in LL; P = 0.243), achieved in a single session in 73.5 % of patients (77.1 % by EHL and 70 % by LL; P= 0.5).D-SOP failed in 11 patients (10.1 %); 6 patients were treated with extracorporeal shockwave lithotripsy (ESWL), 1 with surgery,1 with combined treatment (ESWL + D-SOP EHL) and 3 with other. Fourteen adverse events occurred in 11 patients (10.1 %). Patients with more than three ductal stones were more likely to have technical failure compared to those with less than three stones (17 % vs. 4.8 %; P = 0.04). Having more than three stones was independently associated with the need for more than one D-SOC EHL/LL session (OR 2.94, 95 % CI 1.13 – 7.65).
Conclusion D-SOP with EHL or LL is effective and safe in patients with pancreatic ductal stones.
Electrophilic Amination: An Update
Authors: Zhou Zhe , Kürti László 
Published: July 2019

In this account, we provide an overview of some recent advances in electrophilic amination methodologies that have been developed in the Kürti group over the last seven years. Our group’s focus has been to develop novel amination methodologies that directly yield N-unprotected amine products.1	Introduction2	Amination of Boronic Acids3	Aziridination of Unactivated Olefins4	Rhodium-Catalyzed C–H Amination of Arenes5	Synthesis of Carbazoles6	Amination of Aryl- and Alkylmetals7	Doubly Electrophilic N-Linchpin Reagents8	Aza-Rubottom Oxidation of Silyl Enol Ethers9	Summary
New approach to evaluating the effects of a drug on protein complexes with quantitative proteomics, using the SILAC method and bioinformatic approach
Authors: Kurokawa Natsuki , Kishimoto Taro , Tanaka Kohei , Kondo Jun , Takahashi Nobuhiro , Miura Yutaka 
Published: July 2019

ABSTRACTProtein–protein interactions (PPIs) lead the formation of protein complexes that perform biochemical reactions that maintain the living state of the living cell. Although therapeutic drugs should influence the formation of protein complexes in addition to PPI network, the methodology analyzing such influences remain to be developed. Here, we demonstrate that a new approach combining HPLC (high performance liquid chromatography) for separating protein complexes, and the SILAC (stable isotope labeling using amino acids in cell culture) method for relative protein quantification, enable us to identify the protein complexes influenced by a drug. We applied this approach to the analysis of thalidomide action on HepG2 cells, assessed the identified proteins by clustering data analyses, and assigned 135 novel protein complexes affected by the drug. We propose that this approach is applicable to elucidating the mechanisms of actions of other therapeutic drugs on the PPI network, and the formation of protein complexes.
Green’s function molecular dynamics including viscoelasticity
Authors: van Dokkum Jan Steven , Nicola Lucia 
Published: July 2019

Abstract
The contact mechanical response of various polymers is controlled by the viscoelastic behavior of their bulk and the adhesive properties of their interface. Due to the interplay between viscoelasticity and adhesion it is difficult to predict the contact response, even more when surfaces are rough. Numerical modeling could be of assistance in this task, but has so far mostly dealt with either adhesion or viscoelasticity and focused on simple geometries. Ideally, one would need a model that can concurrently describe viscoelasticity, surface roughness, and interfacial interactions. The numerical technique named Green’s function molecular dynamics (GFMD) has the potential to serve this purpose. To date, it has been used to model contact between adhesive elastic bodies with self-affine surfaces. Here, as a first step, we extend the GFMD technique to include the transient contact response of frictionless viscoelastic bodies. To this end, we derive the constitutive equation for a viscoelastic semi-infinite body in reciprocal space, then integrate it using the semi-analytical method, and find the quasi-static solution through damped dynamics of the individual modes. The new model is then applied to study indentation as well as rolling of a rigid cylinder on a frictionless isotropic half-plane that follows the Zener model when loaded in shear. Extension of the method to a generalized viscoelastic model is straightforward, but the computational effort increases with the number of time-scales required to describe the material. The steady-state response of the rolling cylinder was provided analytically by Hunter in the sixties. Here, we use his analytical solution to validate the steady-state response of our model and provide additionally the transient response for bodies with various shear moduli.
Grouping the districts in Indonesia based on value of science subjects of National Exam using K-Means clustering method
Authors: Ferdhiana R , FAbidin T , Mardhiah A 
Published: July 2019

Abstract
Implementation of the National Examination (NE) for senior high schools for each district in Indonesia is done as one way to mapping the success of education in each the district. Mapping can be done by grouping districts based on attribute values obtained on the implementation of the NE, which in this study using the K-means clustering method. The attribute of NE values used are the values for the science majors, which consist of 6 subjects, where from 514 districts only 510 districts own it. The purpose of the study in addition to grouping districts, will also predict the group of 4 districts that have no NE values. Prediction is done by entering the value of the district Human Development Index (HDI) that has no UN value into 95% confidence interval of HDI from groups formed from k-means clustering. Once known where the district is in a group based on the HDI group then the value of its UN will follow the confidence interval of the UN value of the group. The results of this study indicate that the number of clusters (k) selected to classify districts in Indonesia as many as 5 clusters. Group prediction results show that one district belongs to the V cluster, while the other three districts are predicted to be members of the I cluster.
The potential of five therapeutic medicinal herbs for dental treatment : A review
Authors: Ningsih D S , Idroes R , Bachtiar B M , Khairan  
Published: July 2019

Abstract
Indonesia, especially in Aceh Province, has a huge biodiversity of natural resources in herbal medicine. Calotrophis gigantea (L), Acrostichum aureum (L), Plucheaindica (L), Cissus adnata and Abutilon indicum (L) are considered to have secondary metabolite that are potential for dental therapeutic treatments because the compounds have anti-inflammatory, antibacterial, antifungal, antioxidant, antiseptic and wound healing ability. The utilization of medicinal herbs in dentistry is still limited, thus in this study we provide valuable information of each five medicinal herbs for dental therapeutic treatments. The results indicated that all the five medicinal herbs have different abilities and potentials in treating diseases / abnormalities in the oral cavity. The selection and the use of appropriate medicinal herbs can give an effective therapeutic treatment to improve the health of oral cavity. The qualities of the five herbs are directly proportional to their ability as an alternative therapeutics in dentistry. To produce a good herbal medicines in dentistry, a lot of supporting researches are needed.
1
Geochemistry of warm springs in the Ie Brôuk hydrothermal areas at Aceh Besar district
Authors: Idroes R , Yusuf M , Alatas M , Subhan  , Lala A , Muslem  , Suhendra R , Idroes G M , Suhendrayatna  , Marwan  , Riza M 
Published: July 2019

Abstract
Indonesia is a country with the largest geothermal potential resource in the world, estimated of 28,617 MW or equivalent to 40% of the world’s geothermal potential. Geothermal energy is environmentally friendly and quite economical compared to fossil energy. Seulawah Agam is one of Indonesia’s geothermal fields that has several manifestation zones, including Ie Brôuk (BH1 and BH2) manifestations. The analysis for this study was conducted by acid-base titration, UV-Vis Spectrophotometry and Atomic Absorption Spectroscopy (AAS). The data analysis of cations and anions were plotted in liquid chemistry worksheet plotting spreadsheet version 3 Powell geoscience September 2012 by Powell and Cumming. The type of manifestation fluid is bicarbonate, obtained from the geoindicator Cl-SO4-HCO3, while the water condition is immature based on Na-K-Mg ternary diagram. The Ie Brôuk reservoir temperature was 289 °C and 291 °C obtained from Na/K geothermometer Tonani (1980) and Na/K Giggenbach (1988) respectively. Therefore, the Ie Brôuk manifestation classified as a high-temperature geothermal system.
Antibacterial cream formulation of ethanolic Pliek U extracts and ethanolic residue hexane Pliek U extracts against Staphylococcus aureus
Authors: Rahmad R. , Earlia N , Nabila C , Inayati I , Amin M , Prakoeswa C R S , Khairan K , Idroes R 
Published: July 2019

Abstract
Indonesia as the largest archipelago country in the world is also known as a country with plenty of natural resources that can be used as traditional medicinal plants. Pliek U is a fermented crude coconut meat has been used as an ingredient in Aceh traditional food and also used as a traditional topical medicine in Aceh. Pliek U is potential as a source of free fatty acids (FFAs) such as lauric acid and monolauric acid which have antimicrobial properties. The aim of this study is to determine the activity ethanolic Pliek U extracts (EPUE) and ethanolic of residue hexane of Pliek U extracts (ERHPUE) and also the activities of their cream formulation against Staphylococcus aureus The antibacterial activity was determined by disc difussion method with clindamycin as a positive control. The antibacterial activity results showed that EPUE has antibacterial activity against Staphylococcus aureus with diameter inhibition zone of 8.66±0.57 mm, while ERHPUE and their cream have no activity against Staphylococcus aureus.
Isolation and identification of Endophytic Fungus Fusarium sp from Agarwood (Aquilaria sp) population originated from the forest of Aceh Tamiang district, Indonesia
Authors: Zulfendi  , Idroes Rinaldi , Khairan  
Published: July 2019

Abstract

Aquilaria sp is one of higher plant types that can produce agarwood. Agarwood is commonly stimulated by the biological response of endophytic fungal infection of Fusarium sp. This research aims to isolate and identify endophytic fungus Fusarium sp from Aquilaria sp populations originated from the forest of Aceh Tamiang District. The pure isolates of Fusarium sp derived from Aquilaria sp that reinfected on Aquilaria sp populations to stimulate agarwood formation. The endophytic fungal isolates of Fusarium sp were growth on a selective media of Sabouraud Dextrose Agar (SDA) containing antibiotics Streptomycin 10 µg/dl and Chloramphenicol 25µg/dl. Ten percent of infected Aquilaria sp from the population was taken aseptically. Isolation and identification of endophytic fungus Fusarium sp were conducted from June to August 2016. The results showed that within 10 infected Aquilaria sp, several microbe species were found such as three Fusarium sp, six Aspergillus sp, two Rhizopus sp, two Penicillium sp, one Bacillus sp, one Actinomyces sp and one Streptomyces sp species. The result of identification towards three species of endophytic fungus Fusarium sp that has been purified were srongly suspected to be Fusarium solani, Fusarium oxysporum, and Fusarium monyliformae.