bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel Neuroprotective Loci Modulating Ischemic Stroke Volume in Wild-Derived Inbred Mouse Strains
Authors: Lee Han Kyu , Widmayer Samuel J , Huang Min-Nung , Aylor David L , Marchuk Douglas A 
Published: September 2019

AbstractTo identify genes involved in cerebral infarction, we have employed a forward genetic approach in inbred mouse strains, using quantitative trait loci (QTL) mapping for cerebral infarct volume after middle cerebral artery occlusion. We had previously observed that infarct volume is inversely correlated with cerebral collateral vessel density in most strains. In this study, we expanded the pool of allelic variation among classical inbred mouse strains by utilizing the eight founder strains of the Collaborative Cross and found a wild-derived strain, WSB/EiJ, that breaks this general rule that collateral vessel density inversely correlates with infarct volume. WSB/EiJ and another wild-derived strain, CAST/EiJ, show the highest collateral vessel densities of any inbred strain, but infarct volume of WSB/EiJ mice is 8.7-fold larger than that of CAST/EiJ mice. QTL mapping between these strains identified four new neuroprotective loci modulating cerebral infarct volume while not affecting collateral vessel phenotypes. To identify causative variants in genes, we surveyed nonsynonymous coding SNPs between CAST/EiJ and WSB/EiJ and found 96 genes harboring coding SNPs predicted to be damaging and mapping within one of the four intervals. In addition, we performed RNA-sequencing for brain tissue of CAST/EiJ and WSB/EiJ mice and identified 79 candidate genes mapping in one of the four intervals showing strain-specific differences in expression. The identification of the genes underlying these neuroprotective loci will provide new understanding of genetic risk factors of ischemic stroke, which may provide novel targets for future therapeutic intervention of human ischemic stroke.
Preishöhenmissbrauch durch Verweigerung einer Entgeltanpassung in langfristigen Verträgen
Authors: Fuchs Andreas 
Published: September 2019

Zusammenfassung
Das Urteil des BGH vom 24. 1. 2017 (KZR 2/15) im Fall „Kabelkanalanlagen“ hat den langjährigen Streit um die Höhe der Mietzahlungen für den Zugang zu Kabelschächten zwischen den Betreibern von Breitbandkabelnetzen und der Telekom Deutschland nicht beendet. Die Entscheidung ist nicht nur in der Literatur auf ein geteiltes Echo gestoßen, sondern hat in der Sache auch nicht die Gefolgschaft des OLG Frankfurt/M. und – in einem Parallelverfahren – des OLG Düsseldorf gefunden. Vielmehr haben diese Berufungsgerichte im Gegensatz zum BGH einen Marktmachtmissbrauch durch das Festhalten an dem noch vor Erlangung einer marktbeherrschenden Stellung des Eigentümers der Kabelkanalanlagen vereinbarten Mietzins, der inzwischen erheblich über dem aktuellen wettbewerbsanalogen Preis liegt, verneint. Dabei haben sie sich im Rahmen der gebotenen umfassenden Interessenabwägung auch auf grundsätzliche Überlegungen zum Verhältnis der kartellrechtlichen Preiskontrolle zur Vertragsfreiheit sowie zum Grundsatz pacta sunt servanda gestützt, die ihrerseits Fragen aufwerfen. Vor diesem Hintergrund geht der vorliegende Beitrag der Problematik nach, ob und ggf. unter welchen Voraussetzungen langfristige Verträge, die noch unter Wettbewerbsbedingungen abgeschlossen worden sind, nach Erlangung einer marktbeherrschenden Stellung der kartellrechtlichen Preishöhenkontrolle gem. § 19 Abs. 1, 2 Nr. 2 GWB unterliegen und die Verweigerung der Anpassung eines ungekündigten Vertrags als missbräuchliche Verhaltensweise qualifiziert werden kann.
Die Neujustierung der fusionskontrollrechtlichen Aufgreifschwellen in der 10. GWB-Novelle
Authors: Stadler Christoph 
Published: September 2019

Zusammenfassung
Dieser Beitrag geht der Frage nach, wie sich die Fallbelastung und die Interventionsrate des Bundeskartellamts sowie die Aufgreifschwellen der deutschen Fusionskontrolle im historischen Zusammenhang und internationalen Vergleich darstellen, und von welchen Erwägungen sich der Gesetzgeber bei der anstehenden Neujustierung der Schwellenwerte leiten lassen sollte. Dabei liegt der Fokus auf den „traditionellen“ Aufgreifschwellen des § 35 Abs. 1 GWB. Die Überlegungen bewegen sich innerhalb des bestehenden Koordinatensystems der präventiven Fusionskontrolle und verzichten auf eine Diskussion grundsätzlich bestehender Alternativen, wie z. B. eine von Aufgreifschwellen unabhängige Untersuchungsbefugnis der Kartellbehörde.
Celiac Disease Autoimmunity and Emotional and Behavioral Problems in Childhood
Authors: Wahab Rama J. , Beth Sytske A. , Derks Ivonne P.M. , Jansen Pauline W. , Moll Henriëtte A. , Kiefte-de Jong Jessica C. 
Published: September 2019

BACKGROUND AND OBJECTIVES:
Celiac disease (CeD) is associated with psychopathology in children. It is unknown whether this association is present in children with celiac disease autoimmunity (CDA) identified by screening. We examined the associations between subclinical CDA and emotional and behavioral problems in children without previous CeD diagnosis.

METHODS:
In a population-based cohort study of 3715 children (median age: 6 years), blood titers of tissue transglutaminase autoantibodies were analyzed. CDA was defined as a measurement of tissue transglutaminase autoantibodies ≥7 U/mL (n = 51). Children with previous CeD diagnosis or children on a gluten-free diet, were excluded. The Child Behavior Checklist (CBCL) was filled in by parents and was used to assess behavioral and emotional problems of children at a median age of 5.9 years. Multiple linear regression models were applied to evaluate the cross-sectional associations between CDA and CBCL scores. Sensitivity analyses were done in a subgroup of children who were seropositive carrying the HLA antigen risk alleles for CeD.

RESULTS:
In basic models, CDA was not associated with emotional and behavioral problems on the CBCL scales. After adjustment for confounders, CDA was significantly associated with anxiety problems (β = .29; 95% confidence interval 0.02 to 0.55; P = .02). After exclusion of children who did not carry the HLA-DQ2 and/or HLA-DQ8 risk alleles (n = 4), CDA was additionally associated with oppositional defiant problems (β = .35; 95% confidence interval 0.02 to 0.69). Associations were not explained by gastrointestinal complaints.

CONCLUSIONS:
Our results reveal that CDA, especially combined with the HLA-DQ2 and HLA-DQ8 risk alleles, is associated with anxiety problems and oppositional defiant problems. Further research should be used to establish whether behavioral problems are a reflection of subclinical CeD.
Background for the formation and predictors for the implementation of state anti-corruption policy in Ukraine
Authors: Крушельницька Т. А. 
Published: September 2019

Corruption in the public administration of Ukraine, which is a socio-economic phenomenon, is investigated as a plurality of actions of public service officials, which is arising in the process of realization of their authorities and building relationships and connecting with abuse of political or public authority for the sake of personal gain. An analysis of the preconditions for formation and theoretical substantiation of the predictors of the state anti-corruption policy implementation is conducted. Corruption is manifested through such institutional deformations as institutional constraints, legal burdens, distortions of moral and ethical standards of officials, and tolerance of corruption by society.The main preconditions for the formation of anticorruption policy are as follows: the set of external and internal factors of the mechanisms of public and managerial activity, which is burdened by the historical legacy of the administrative-command system of the Soviet era; formation of the outlook of a civil servant, in which the common purpose of occupying a post is a phenomenon like sinecure; a feature of professional state-management activity, which is determined by the state-power authorities of individuals, which are the exclusive privilege and responsibility of officials; the existence of legal preferences that imply inequality of citizens in the access to socially useful benefits that distort understanding of the legal equality of citizens and are the basis of corruption; the presence of effective foreign experience in the implementation of anticorruption policy, based on the cooperation of government institutions with the private sector and the public, is long-term and complex.The main predictors that will become qualitative and quantitative benchmarks for changing the trend in the implementation of anti-corruption policies are reducing the proportion of actions committed to attract corruption leverage, reducing the share of the shadow economy, raising the index of perceived corruption in Ukraine for five years at least to an average of 60-65, the creation of prerequisites for absolute rejection of corruption, complete lack of tolerance to any, even minimal, manifestations of corruption.
Features of modern models of public administration
Authors: Сьомич М. І. 
Published: September 2019

The emergence of the latest nature of public administration is one of the main instruments for the approval of a public governance model, which implies the need to replace bureaucratic mechanisms with socially-oriented market relations.
Level of scientific reflection there is an expansion of the range of applicability of existing concepts and the development of new, increasing hybridization of public administration systems.
The purpose of the article is to identify features and generalized characteristics of the main models of public administration and to formulate proposals for the formation of the most optimal model of public administration in accordance with the stages of transformation of the domestic society.
Old Public Management – the classic bureaucratic form of organization described by Max Weber as the ideal type of rational form of government based on the principle of law-making, with a high level of predictability for both policy in general and for citizens.
New Public Management is a modern model of public administration reform. An important place within the framework of this model is the concept of Digital Government, Network model of government, Anti-crisis Public Administration, Open Government Partnership.
It should be noted that in the "pure" form, these models of public administration are almost never found in any country in the world, although this or that set of elements of certain models is introduced into the practice of modern state regulation. In the majority of foreign countries there is currently a certain tendency towards convergence, a kind of diffusion of various approaches to public administration.
From the elements of these concepts one can form the theoretical basis for the "ideal model" of public administration, which can then be used to study administrative reforms in different countries.
The main essential features of the "ideal model" of a public administration model are to justify the following: implementation of the concept of "good" public administration as part of the Good Governance model, system simplification of legislation (as one of the elements and inherent tools of a "new" public management model), reduction the total number and narrowing of segments of the subject-object area of ​​public administration with the transfer of public functions and public authority by the public administration, ensuring subsidiarity in public-private services development, public-private partnership development, de-bureaucratization and de-concentration of public sector entities, activation of the interaction of public administration and other types of social management, realization and progressive development of the concept of "electronic state", significant increase of requirements for transparency and accountability of public administration, development tools of public administration, increase the variety of their modifications and complication of their combinations, implementation of the concept of management of results, increase ting susceptibility and complementarity against the public interest.
Qualidade de vida de um grupo de idosos vinculados a um plano de saúde na cidade de São Paulo
Authors: Oliveira Henrique Souza Barros de , Fenner Fernanda , Manso Maria Elisa Gonzalez 
Published: September 2019

AIM: This study aims to assess the life quality of a group of elderly people linked to a health plan in the city of São Paulo and to verify which variables affect this construct. METHODS: This is an exploratory cross-sectional study carried out in 2017 with a group of elderly people over 60 years of age and without cognitive deficits, linked to a health plan operator, all residents of the city of São Paulo, SP. The WHOQOL-BREF and WHOQOL-Old self-filled questionnaires were applied and the elderly participants also answered questionnaires relating to sociodemographic, morbidities and the usage of services developed by the researchers. RESULTS: The research group evaluates their quality of life as good and is satisfied with their health, but some points stand out. For said group, living by themselves, having cardiocirculatory diseases or having more than one chronic disease influenced negatively their quality of life, whereas being in a stable unionship and having access to consultations with intervals below six months interfered positively. It was observed that the group was concerned about issues related to independence, communication capacity, social life, and death. The environment and intimacy were shown as highlights for this group. CONCLUSIONS: It was verified in this group of elderly people that the best quality of life performance was obtained in the Environment and Social Relations domains and in the Intimacy and Past, Present and Future aspects. In addition, sociodemographic, morbidity and health service use variables significantly affected this construct. The characteristics of the research group do not allow us to generalize the findings, but it is hoped to have contributed with the look on a group as diverse as the Brazilian elderly.
Genotoxic effects of mercury chloride on the Neotropical fish Andinoacara rivulatus (Cichlidae: Cichlasomatini)
Authors: Nirchio Mauro , Choco Ventimilla Oscar Jesús , Quizhpe Cordero Patricio Freddy , Hernández José Gregorio , Oliveira Claudio 
Published: September 2019

Understanding the effects of heavy metals in aquatic ecosystems is of significant importance due to their potential to bioaccumulate at various trophic levels and induce damage in DNA. Mercury is considered one of the most dangerous heavy metals, causing chromosomal breakage (clastogenic event) or spindle dysfunction (aneugenic event), that can lead to the formation of encapsulated chromatin into a separate smaller nucleus, generally referred to as a micronucleus. We evaluated the sensitivity of the micronucleus test in the neotropical cichlid Andinoacara rivulatus (Günther 1860). The fish were divided into four groups of 16 individuals, and each group was placed in separate aquaria (140 L) provided with filtered water and constant aeration. Fish were exposed to mercury chloride (HgCl2) at doses 0.1, 0.25, and 0.50 mg/Kg body weight, administered by intraperitoneal (IP) injection. Fish from the control group were injected with a physiologic solution. The following erythrocyte anomalies were identified: erythrocytes with micronuclei varying to some extent in size and position in the cytoplasm, blebbed nucleus, binucleated cell, nuclei showing a deep invagination toward the center (notched nuclei). Examination of blood smears demonstrated a higher level of micronucleus and notched erythrocytes in fish injected with HgCl2 than in the controls. There were significant differences in the frequency of micronucleated and notched erythrocyte among the groups exposed to mercury. Linear regression analysis revealed a positive relationship between the frequency of micronucleated and notched erythrocytes (P < 0.0001), with a moderately strong correlation coefficient (r=0.737). We propose that, in addition to the two so far known mechanisms of micronucleus formation (spindle apparatus damage and chromosomal ruptures), chromatin fragmentation in notched nuclei resulting from a combination of the cytotoxic effects of mercury and mechanical stress, may be a third mechanism of micronuclei genesis. It is emphasized that although the micronucleus test is a cheap and technically simple method that requires very basic equipment, direct counting of erythrocytes using a microscope is a time-consuming task because it requires counting a high number of cells (a minimum of 1,000 to 2,000 cells) per blood smear, leading to visual fatigue. We recommend, photographing the stained blood smears at several areas at random and scoring the digital images using Adobe Photoshop software as an easy way to evaluate a large number of erythrocytes for minimizing the visual fatigue caused by many hours of direct observation through a microscope
Rapid test for the qualitative simultaneous determination of cardiac fatty acid-binding protein and cardiac troponin I in the diagnosis of acute coronary syndrome
Authors: Kokorin V. A. , Gordeev I. G. , Arefyev M. N. , Goncharova A. Ya. , Yakovtsova A. A. 
Published: September 2019

Aim.To study the diagnostic characteristics of the rapid test for qualitative simultaneous determination of cardiac fatty acid-binding protein (FABPs) and cardiac troponin I (cTnI) CARD-INFO 1+1 in patients with acute coronary syndrome (ACS).Material and methods.The study included 168 patients undergoing inpatient treatment after ACS, with typical anginal pain lasting at least 20 minutes occurred in the previous 1-24 hours. In addition to routine diagnostic procedures, on admission, we determined FABPs and cTnI concentrations using the high-quality immunochromatographic rapid test CARD-INFO 1 1 (OOO CARDIO-Plus, Russia).Results.The sensitivity of the CARD-INFO 1+1 rapid test was 88,1%, specificity — 89,8%, diagnostic accuracy — 88,7%. The indicators of the diagnostic effectiveness of CARD-INFO 1+1 test in patients with STE-ACS and NSTE-ACS did not significantly differ (p>0,05). The sensitivity of the rapid test reached a maximum in the period from 3 to 6 hours from the onset of pain. Compared with the determination of cTnI performed on admission to the hospital, a higher sensitivity of the CARD-INFO 1+1 test was revealed in patients with STE-ACS (87,7% vs 75,3%; p=0,044), in the first 1-3 hours after the beginning of clinical manifestations (86,8% vs 60,5%; p=0,041) and in the entire sample as a whole (88,1% vs 77,1%; p=0,033), with comparable specificity (89,8% and 93,2%, respectively; p=0,741).Conclusion.Qualitative immunochromatographic CARD-INFO 1+1 rapid test for the simultaneous determination of the content of FABPs and cTnI I is highly effective in the diagnosis of various forms of ACS. The highest diagnostic characteristics of the test were observed in patients in the early stages of the disease (the first 1-6 hours after the onset of pain). Carrying out the CARD-INFO 1+1 test revealed 12 MI cases more (11%) than the first determination of cTnI. Further studies will clarify the place of this technique in the modern algorithm for the management of patients with ACS and evaluate the possibility of using the rapid test in predicting the course of the disease.
Clinical Benefit of Sorafenib Combined with Paclitaxel and Carboplatin to a Patient with Metastatic Chemotherapy-Refractory Testicular Tumors
Authors: Lian Bijun , Zhang Wenhui , Wang Tiegong , Yang Qingsong , Jia Zepeng , Chen Huan , Wang Lei , Xu Jing , Wang Wei , Cao Kai , Gao Xu , Sun Yinghao , Shao Chengwei , Liu Zhiyong , Li Jing 
Published: September 2019

Abstract

Testicular cancer is one of the few tumor types that have not yet benefited from targeted therapy. Still no new active agents for treating this cancer have been identified over the past 15 years. Once patients are refractory to cisplatin-based chemotherapy, they will be expected to die from testicular cancer. This report describes a 21-year-old man who was refractory to chemotherapy and immunotherapy. Whole exome sequencing and low-depth whole genome sequencing confirmed the KRAS gene amplification, which may lead to the tumor cells’ progression and proliferation. After discussion at the molecular tumor board, the patient was offered paclitaxel, carboplatin, and sorafenib (CPS) based on a phase III clinical trial of melanoma with KRAS gene copy gains. After treatment with CPS, the patient achieved excellent curative effects. Because of a nearly 50% frequency of KRAS amplification in chemotherapy-refractory testicular germ cells, CPS regimen may provide a new therapy, but it still warrants further validation in clinical studies.

Key Points
Chemotherapy-refractory testicular cancer has a very poor prognosis resulting in a lack of effective targeted therapies. KRAS gene amplification occurs in nearly 20% of testicular cancer and 50% of chemotherapy-refractory testicular cancer. KRAS amplification may activate the MAPK signaling pathway, and inhibition of MAPK by sorafenib combined with paclitaxel and carboplatin could be a viable option based on a phase III clinical trial of melanoma. To the authors’ knowledge, this is the first report of response to sorafenib-based combination targeted therapy in a patient with chemotherapy-refractory testicular cancer. Clinical genomic profiling can confirm copy number variation of testicular cancer and provide insights on therapeutic options.
Optimization of early diagnosis of ovarian cancer using an original software ScOv
Authors: Khabarov S. V. , Nikogosyan S. O. , Volkov V. G. , Chibisova G. M. 
Published: September 2019

The most common causes of treatment failure in ovarian cancer (ОС) include late diagnosis due to the absence of screening programs for its early detection and lack of vigilance by general practitioners and gynecologists. World experience suggests that screening of 100% of women has a minimal impact on mortality. Examination of women at high risk for OC is a sensible alternative to mass screening. This examination can be performed using universal computerized screening, which is a very promising and cost-effective method.To identify the symptoms significantly associated with early-stage OC, we questionnaired 100 patients with morphologically verified stage IA–IC OC, admitted to the Department of Gynecology, Tula Regional Oncology Dispensary between 2010 and 2017 (experimental group). The control group included 200 women without malignant diseases, who underwent preventive medical examination in outpatient units of Tula. We analyzed the significance of 22 clinical symptoms and developed original computer software ScOv, which was subsequently used for identifying patients at high risk of OC among women aged 40 years and older by evaluating their complaints. The sensitivity and specificity of ScOv were 73.7 % and 88.8 % respectively. A retrospective check of the developed program demonstrated its high efficiency and prognostic value.
Clinical application of minimal invasive arthroscope on patella fracture surgery
Authors: Jiang Bo , Liao Lele 
Published: September 2019

<p class="p1">The aim of the research is to perform the application of minimal invasive arthroscope on patella fracture surgery. A total of 100 patients with the cases of patella fracture were selected from our hospital and the Second Xiangya Hospital’s Orthopaedic Ward. These patients were divided into ‘Observation Group’ and ‘Comparison Group’. The former was treated with traditional open surgery whereas the latter used the arthroscopic surgery. The postsurgical score indicated statistically significant differences (P &lt; 0.05) in the Lysholm Knee and Oswestry Low Back pain scale. By performing arthroscopic surgery on patella fractures, the patients’ recovery was accelerated while the pain was greatly reduced, which in turn, improved the quality of patients’ life and provided valuable clinical value. </p>