bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-sectional survey on adult acute kidney injury in Chinese ICU: the study protocol (CARE-AKI)
Authors: Yang Zhenyu , Wang Cong , Wang Hongliang , Wang Sicong , Liu Ruijin , Wang Xu , Yu Kaijiang 
Published: June 2018

IntroductionAcute kidney injury (AKI) is one of the most serious syndromes in intensive care unit (ICU) patients, and is a mysterious problem in clinical practice worldwide. Due to unknown aetiology and mechanism, awareness of AKI diagnosis and treatment in China varies, resulting in underestimated incidence and poor prognosis. To solve this problem, we design this national survey of AKI in adult ICUs. Various indexes are included and analysed to classify the epidemiology of adult AKI in Chinese ICUs, including AKI aetiology, risk factors, mortality, prognosis, therapeutic strategies and cognition of ICU medical staff.MethodsA multicentre, cross-sectional survey, which will involve about 35 hospitals and 6147 patients from 23 provinces, 4 municipalities and 5 autonomous regions, is planned. All patients who meet the inclusion criteria are eligible to apply for enrolment in the study, which cover baseline demographics, clinical performance, and follow-up related to diagnosis and treatment.ConclusionThe study is expected to fill the gap between China and developed countries, and to provide a theoretical foundation for developing more scientific and standardised approaches to AKI diagnosis and treatment.Ethics and disseminationEthical approval was obtained from the ethics committee of Harbin Medical University Cancer Hospital (registration number KY2017-21). The findings of this review will be communicated through peer-reviewed publications and scientific presentations.Trial registration numberChiCTR-EOC-17013133; Pre-results.
Feasibility study to assess the delivery of a lifestyle intervention (TreatWELL) for patients with colorectal cancer undergoing potentially curative treatment
Authors: Macleod Maureen , Steele Robert J C , O’Carroll Ronan E , Wells Mary , Campbell Anna , Sugden Jacqui A , Rodger Jackie , Stead Martine , McKell Jennifer , Anderson Annie S 
Published: June 2018

ObjectivesTo assess the feasibility of delivering and evaluating a lifestyle programme for patients with colorectal cancer undergoing potentially curative treatments.Study designNon-randomised feasibility trial.SettingNational Health Service (NHS) Tayside.ParticipantsAdults with stage I–III colorectal cancer.InterventionThe programme targeted smoking, alcohol, physical activity, diet and weight management. It was delivered in three face-to-face counselling sessions (plus nine phone calls) by lifestyle coaches over three phases (1: presurgery, 2: surgical recovery and 3: post-treatment recovery).Primary outcomeFeasibility measures (recruitment, retention, programme implementation, achieved measures, fidelity, factors affecting protocol adherence and acceptability).Secondary outcomesMeasured changes in body weight, waist circumference, walking and self-reported physical activity, diet, smoking, alcohol intake, fatigue, bowel function and quality of life.ResultsOf 84 patients diagnosed, 22 (26%) were recruited and 15 (18%) completed the study. Median time for intervention delivery was 5.5 hours. Coaches reported covering most (>70%) of the intervention components but had difficulties during phase 2. Evaluation measures (except walk test) were achieved by all participants at baseline, and most (<90%) at end of phase 2 and phase 3, but <20% at end of phase 1. Protocol challenges included limited time between diagnosis and surgery and the presence of comorbidities. The intervention was rated highly by participants but limited support from NHS staff was noted. The majority of participants (77%) had a body mass index>25 kg/m2and none was underweight. Physical activity data showed a positive trend towards increased activity overall, but no other changes in secondary outcomes were detected.ConclusionsTo make this intervention feasible for testing as a full trial, further research is required on (a) recruitment optimisation, (b) appropriate assessment tools, (c) protocols for phase 2 and 3, which can build in flexibility and (d) ways for NHS staff to facilitate the programme.Trial registration numberISRCTN52345929; Post-results.
Well-being among survivors of out-of-hospital cardiac arrest: a cross-sectional retrospective study in Sweden
Authors: Viktorisson Adam , Sunnerhagen Katharina S , Pöder Ulrika , Herlitz Johan , Axelsson Åsa B 
Published: June 2018

ObjectivesThe psychological outcome of out-of-hospital cardiac arrest (OHCA) has been studied more extensively in recent years. Still, not much is known about the well-being among OHCA survivors. In this retrospective cross-sectional study, we aim to investigate post-OHCA well-being among patients with a good neurological outcome, 3 months after the cardiac event. To assess well-being, we analyse the frequency of anxiety, depression, post-traumatic stress disorder (PTSD) and health within this group. Further, we aim to evaluate the importance of five prognostic factors for post-OHCA well-being.MethodsData collection took place between 2008 and 2012, and every OHCA survivor within one region of Sweden, with a cerebral performance category (CPC) score of ≤2 at discharge, was asked to participate. Survivors were identified through the Swedish Cardiopulmonary Resuscitation Registry, and postal questionnaires were sent out 3 months after the OHCA. The survey included Hospital Anxiety and Depression scale (HADS), PTSD Checklist Civilian version (PCL-C) and European Quality of Life 5 Dimensions 3 level (EQ-5D-3L).ResultsOf 298 survivors, 150 were eligible for this study and 94 responded. The mean time from OHCA to follow-up was 88 days. There was no significant difference between respondents and non-respondents in terms of sex, age, cardiac arrest circumstances or in-hospital interventions. 48 participants reported reduced well-being, and young age was the only factor significantly correlated to this outcome (p=0.02). Women reported significantly higher scores in HADS (p=0.001) and PCL-C (p<0.001). Women also reported significantly lower EQ-5D index values (p=0.002) and EQ-visual analogue scale scores (p=0.002) compared with men.ConclusionReduced well-being is experienced by half of OHCA survivors with a CPC score ≤2, and young age is negatively correlated to this outcome. The frequency of anxiety and PTSD is higher among women, who also report worse health.
Poisson Sigma Model and Semiclassical Quantization of Integrable Systems
Authors: Cattaneo Alberto S. , Mnev Pavel , Reshetikhin Nicolai 
Published: June 2018

In this paper, we outline the construction of semiclassical eigenfunctions of integrable models in terms of the semiclassical path integral for the Poisson sigma model with the target space being the phase space of the integrable system. The semiclassical path integral is defined as a formal power series with coefficients being Feynman diagrams. We also argue that in a similar way one can obtain irreducible semiclassical representations of Kontsevich’s star product. Dedicated to the memory of L. D. Faddeev
Wilson Loops in Antisymmetric Representations from Localization in Supersymmetric Gauge Theories
Authors: Russo Jorge G. , Zarembo Konstantin 
Published: June 2018

Large-[Formula: see text] phase transitions occurring in massive [Formula: see text] theories can be probed by Wilson loops in large antisymmetric representations. The logarithm of the Wilson loop is effectively described by the free energy of a Fermi distribution and exhibits second-order phase transitions (discontinuities in the second derivatives) as the size of representation varies. We illustrate the general features of antisymmetric Wilson loops on a number of examples where the phase transitions are known to occur: [Formula: see text] SQCD with various mass arrangements and [Formula: see text] theory. As a byproduct, we solve planar [Formula: see text] SQCD with three independent mass parameters. This model has two effective mass scales and undergoes two phase transitions. In memory of Ludvig Dmitrievich Faddeev
Lifestyle intervention for morbid obesity: effects on liver steatosis, inflammation, and fibrosis
Authors: Hohenester Simon , Christiansen Simon , Nagel Jutta , Wimmer Ralf , Artmann Renate , Denk Gerald , Bischoff Monika , Bischoff Gert , Rust Christian 
Published: June 2018

The prevalence of obesity-related nonalcoholic fatty liver disease (NAFLD) is rising. NAFLD may result in nonalcoholic steatohepatitis (NASH), progressing to liver cirrhosis. Weight loss is recommended to treat obesity-related NASH. Lifestyle intervention may improve NASH; however, pertinent trials have so far focused on overweight patients, whereas patients with obesity are at highest risk of developing NAFLD. Furthermore, reports of effects on liver fibrosis are scarce. We evaluated the effect of lifestyle intervention on NAFLD in a real-life cohort of morbidly obese patients. In our observational study, 152 patients underwent lifestyle intervention, with a follow-up of 52 weeks. Noninvasive measures of obesity, metabolic syndrome, liver steatosis, liver damage, and liver fibrosis were analyzed. Treatment response in terms of weight loss was achieved in 85.1% of patients. Dysglycemia and dyslipidemia improved. The proportion of patients with fatty liver dropped from 98.1 to 54.3% ( P < 0.001). Weight loss >10% was associated with better treatment response ( P = 0.0009). Prevalence of abnormal serum transaminases fell from 81.0 to 50.5% ( P < 0.001). The proportion fibrotic patients, as determined by the NAFLD fibrosis score, dropped from 11.8 to 0% ( P < 0.05). Low serum levels of adiponectin correlated with degree of liver damage, i.e., serum liver transaminases ( r = −0,32, P < 0.05). Serum levels of adiponectin improved with intervention. In conclusion, lifestyle intervention effectively targeted obesity and the metabolic syndrome. Liver steatosis, damage and fibrosis were ameliorated in this real-life cohort of morbidly obese patients, mediated in part by changes in the adipokine profile. Patients with weight loss of >10% seemed to benefit most.NEW & NOTEWORTHY We demonstrate new evidence that lifestyle intervention is effective in treating NAFLD in the important group of patients with (morbid) obesity. Although current guidelines on the therapy of NASH recommend weight loss of 5–7%, weight reduction >10% may be favorable in morbid obesity. Serum levels of adipokines correlate with liver damage, which is indicative of their pathogenetic importance in human NASH. Our study adds to the limited body of evidence that NAFLD-associated liver fibrosis may resolve with lifestyle intervention.
Effect of healthy aging on cerebral blood flow, CO2 reactivity, and neurovascular coupling during exercise
Authors: Nowak-Flück Daniela , Ainslie Philip N. , Bain Anthony R. , Ahmed Amar , Wildfong Kevin W. , Morris Laura E. , Phillips Aaron A. , Fisher James P. 
Published: June 2018

We sought to make the first comparisons of duplex Doppler ultrasonography-derived measures of cerebral blood flow during exercise in young and older individuals and to assess whether healthy aging influences the effect of exercise on neurovascular coupling (NVC) and cerebral vascular reactivity to changes in carbon dioxide (CVRco2). In 10 healthy young (23 ± 2 yr; mean ± SD) and 9 healthy older (66 ± 3 yr) individuals, internal carotid artery (ICA) and vertebral artery (VA) blood flows were concurrently measured, along with middle and posterior cerebral artery mean blood velocity (MCAvmean and PCAvmean). Measures were made at rest and during leg cycling (75 W and 35% maximum aerobic workload). ICA and VA blood flow during dynamic exercise, undertaken at matched absolute (ICA: young 336 ± 95, older 352 ± 155; VA: young 95 ± 43, older 100 ± 30 ml/min) and relative (ICA: young 355 ± 125, older 323 ± 153; VA: young 115 ± 48, older 110 ± 32 ml/min) intensities, were not different between groups ( P > 0.670). The PCAvmean responses to visual stimulation (NVC) were blunted in older versus younger group at rest (16 ± 6% vs. 23 ± 7%, P < 0.026) and exercise; however, these responses were not changed from rest to exercise in either group. The ICA and VA CVRco2 were comparable in both groups and unaltered during exercise. Collectively, our findings suggest that 1) ICA and VA blood flow responses to dynamic exercise are similar in healthy young and older individuals, 2) NVC is blunted in healthy older individuals at rest and exercise but is not different between rest to exercise in either group, and 3) CVRco2 is similar during exercise in healthy young and older groups. NEW & NOTEWORTHY Internal carotid artery and vertebral artery blood flow responses to dynamic exercise are similar in healthy young and older individuals. Neurovascular coupling and cerebrovascular carbon dioxide reactivity, two key mechanisms mediating the cerebral blood flow responses to exercise, are generally unaffected by exercise in both healthy young and older individuals.
Critical power is positively related to skeletal muscle capillarity and type I muscle fibers in endurance-trained individuals
Authors: Mitchell Emma A. , Martin Neil R. W. , Bailey Stephen J. , Ferguson Richard A. 
Published: June 2018

The asymptote [critical power (CP)] and curvature constant ( W′) of the hyperbolic power-duration relationship can predict performance within the severe-intensity exercise domain. However, the extent to which these parameters relate to skeletal muscle morphology is less clear, particularly in endurance-trained individuals, who, relative to their lesser-trained counterparts, possess skeletal muscles that can support high levels of oxygen transport and oxidative capacity, i.e., elevated type I fiber proportion and cross-sectional area (CSA) and capillarity. Fourteen endurance-trained men performed a maximal incremental test to determine peak oxygen uptake (V̇o2peak; 63.2 ± 4.1 ml·min−1·kg−1, mean ± SD) and maximal aerobic power (406 ± 63 W) and three to five constant-load tests to task failure for the determination of CP (303 ± 52 W) and W′ (17.0 ± 3.0 kJ). Skeletal muscle biopsies were obtained from the vastus lateralis and analyzed for percent proportion of fiber types, CSA, and indexes of capillarity. CP was positively correlated with the percent proportion ( r = 0.79; P = 0.001) and CSA ( r = 0.73; P = 0.003) of type I fibers, capillary-to-fiber ratio ( r = 0.88; P < 0.001), and capillary contacts around type I fibers ( r = 0.94; P < 0.001) and type II fibers ( r = 0.68; P = 0.008). W′ was not correlated with any morphological variables. These data reveal a strong positive association between CP and skeletal muscle capillarity. Our findings support the assertion that CP is an important parameter of aerobic function and offer novel insights into the physiological bases of CP. NEW & NOTEWORTHY This investigation demonstrated very strong positive correlations between critical power and skeletal muscle capillarity, particularly around type I fibers, and type I fiber composition. These correlations were demonstrated in endurance-trained individuals expected to possess well-adapted skeletal muscles, such as high levels of oxygen transport structures and high oxidative capacities, supporting the view that critical power is an important parameter of aerobic function. In contrast, the curvature constant W′ was not associated with fiber type composition or capillarity.
Hypoxic Tumor-Derived Exosomal miR-301a Mediates M2 Macrophage Polarization via PTEN/PI3Kγ to Promote Pancreatic Cancer Metastasis
Authors: Wang Xiaofeng , Luo Guangtao , Zhang Kundong , Cao Jun , Huang Chen , Jiang Tao , Liu Bingya , Su Liping , Qiu Zhengjun 
Published: June 2018

Abstract
Exosomes are emerging as important mediators of the cross-talk between tumor cells and the microenvironment. However, the mechanisms by which exosomes modulate tumor development under hypoxia in pancreatic cancer remain largely unknown. Here, we found that hypoxic exosomes derived from pancreatic cancer cells activate macrophages to the M2 phenotype in a HIF1a or HIF2a–dependent manner, which then facilitates the migration, invasion, and epithelial–mesenchymal transition of pancreatic cancer cells. Given that exosomes have been shown to transport miRNAs to alter cellular functions, we discovered that miR-301a-3p was highly expressed in hypoxic pancreatic cancer cells and enriched in hypoxic pancreatic cancer cell–derived exosomes. Circulating exosomal miR-301a-3p levels positively associated with depth of invasion, lymph node metastasis, late TNM stage, and poor prognosis of pancreatic cancer. Hypoxic exosomal miR-301a-3p induced the M2 polarization of macrophages via activation of the PTEN/PI3Kγ signaling pathway. Coculturing of pancreatic cancer cells with macrophages in which miR-301a-3p was upregulated or treated with hypoxic exosomes enhanced their metastatic capacity. Collectively, these data indicate that pancreatic cancer cells generate miR-301a-3p–rich exosomes in a hypoxic microenvironment, which then polarize macrophages to promote malignant behaviors of pancreatic cancer cells. Targeting exosomal miR-301a-3p may provide a potential diagnosis and treatment strategy for pancreatic cancer.
Significance: These findings identify an exosomal miRNA critical for microenvironmental cross-talk that may prove to be a potential target for diagnosis and treatment of pancreatic cancer.
Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/16/4586/F1.large.jpg. Cancer Res; 78(16); 4586–98. ©2018 AACR.
Hexavalent Chromium–Induced Chromosome Instability Drives Permanent and Heritable Numerical and Structural Changes and a DNA Repair–Deficient Phenotype
Authors: Wise Sandra S. , Aboueissa Abou El-Makarim , Martino Julieta , Wise John Pierce 
Published: June 2018

Abstract
A key hypothesis for how hexavalent chromium [Cr(VI)] causes cancer is that it drives chromosome instability (CIN), which leads to neoplastic transformation. Studies show chronic Cr(VI) can affect DNA repair and induce centrosome amplification, which can lead to structural and numerical CIN. However, no studies have considered whether these outcomes are transient or permanent. In this study, we exposed human lung cells to particulate Cr(VI) for three sequential 24-hour periods, each separated by about a month. After each treatment, cells were seeded at colony-forming density, cloned, expanded, and retreated, creating three generations of clonal cell lines. Each generation of clones was tested for chromium sensitivity, chromosome complement, DNA repair capacity, centrosome amplification, and the ability to grow in soft agar. After the first treatment, Cr(VI)-treated clones exhibited a normal chromosome complement, but some clones showed a repair-deficient phenotype and amplified centrosomes. After the second exposure, more than half of the treated clones acquired an abnormal karyotype including numerical and structural alterations, with many exhibiting deficient DNA double-strand break repair and amplified centrosomes. The third treatment produced new abnormal clones, with previously abnormal clones acquiring additional abnormalities and most clones exhibiting repair deficiency. CIN, repair deficiency, and amplified centrosomes were all permanent and heritable phenotypes of repeated Cr(VI) exposure. These outcomes support the hypothesis that CIN is a key mechanism of Cr(VI)-induced carcinogenesis.
Significance: Chromium, a major public health concern and human lung carcinogen, causes fundamental changes in chromosomes and DNA repair in human lung cells. Cancer Res; 78(15); 4203–14. ©2018 AACR.
MicroRNA-Related Genetic Variants Associated with Survival of Head and Neck Squamous Cell Carcinoma
Authors: Wilkins Owen M. , Titus Alexander J. , Salas Lucas A. , Gui Jiang , Eliot Melissa , Butler Rondi A. , Sturgis Erich M. , Li Guojun , Kelsey Karl T. , Christensen Brock C. 
Published: June 2018

Abstract

Background:
Head and neck squamous cell carcinoma (HNSCC) is commonly diagnosed at an advanced stage, and prognosis for such patients is poor. There remains a gap in our understanding of genetic variants related with HNSCC prognosis. miRNA-related single nucleotide polymorphisms (miR-SNPs) are a class of genetic variants with gene-regulatory potential.

Methods:
We used a genome-scale approach and independent patient populations in a two-stage approach to test 40,286 common miR-SNPs for association with HNSCC survival in the discovery population (n = 847), and selected the strongest associations for replication in validation phase cases (n = 1,236). Furthermore, we leveraged miRNA interaction databases and miRNA expression data from The Cancer Genome Atlas, to provide functional insight for the identified and replicated associations.

Results:
Joint population analyses identified novel miR-SNPs associated with overall survival in oral and laryngeal cancers. rs1816158, located within long noncoding RNA MIR100HG, was associated with overall survival in oral cavity cancer (HR, 1.56; 95% confidence interval (CI), 1.21–2.00). In addition, expression of MIR100HG-embedded miRNA, miR-100, was significantly associated with overall survival in an independent cohort of HNSCC cases (HR, 1.25; 95% CI, 1.06–1.49). A SNP in the 3′UTR of SH3BP4 (rs56161233) that overlaps predicted miRNA-binding sites and is predicted to disrupt several miRNA–mRNA interactions was associated with overall survival of laryngeal cancer (HR, 2.57; 95% CI, 1.71–3.86).

Conclusions:
This work reveals novel miR-SNPs associated with HNSCC survival, and utilizes miRNA-mRNA interaction and expression data to provide functional support for these associations.

Impact:
These findings extend our understanding of how genetic variation contributes to HNSCC survival, and may contribute to future prognostic models for improved risk stratification.
PD-L1 Binds to B7-1 Only In Cis on the Same Cell Surface
Authors: Chaudhri Apoorvi , Xiao Yanping , Klee Alyssa N. , Wang Xiaoxu , Zhu Baogong , Freeman Gordon J. 
Published: June 2018

Abstract
Programmed death ligand 1 (PD-L1)–mediated immunosuppression regulates peripheral tolerance and is often co-opted by tumors to evade immune attack. PD-L1 binds to PD-1 but also binds to B7-1 (CD80) to regulate T-cell function. The binding interaction of PD-L1 with B7-1 and its functional role need further investigation to understand differences between PD-1 and PD-L1 tumor immunotherapy. We examined the molecular orientation of PD-L1 binding to B7-1 using cell-to-cell binding assays, ELISA, and flow cytometry. As expected, PD-L1–transfected cells bound to PD-1–transfected cells, and B7-1 cells bound to CD28 or CTLA-4–transfected cells; however, PD-L1 cells did not bind to B7-1 cells. By ELISA and flow cytometry with purified proteins, we found PD-L1 and B7-1 had a strong binding interaction only when PD-L1 was flexible. Soluble PD-1 and B7-1 competed for binding to PD-L1. Binding of native PD-L1 and B7-1 in cis on the same cell surface was demonstrated with NanoBiT proximity assays. Thus, PD-L1–B7-1 interaction can occur in cis on the same cell but not in trans between two cells, which suggests a model in which PD-L1 can bend via its 11-amino acid, flexible stalk to bind to B7-1 in cis, in a manner that can competitively block the binding of PD-L1 to PD-1 or of B7-1 to CD28. This binding orientation emphasizes the functional importance of coexpression of PD-L1 and B7-1 on the same cell. We found such coexpression on tumor-infiltrating myeloid cells. Our findings may help better utilize these pathways in cancer immunotherapy. Cancer Immunol Res; 6(8); 921–9. ©2018 AACR.