bluuebunny/crossref_metadata_2025_split huggingface dataset:
Portable WIM Systems: Comparison of Sensor Installation Methods for Site-Specific Traffic Data Measurements
Authors: Walubita Lubinda F. , Mahmoud Enad , Fuentes Luis , Komba Julius J. , Teshale Eyoab Zegeye , Faruk Abu N. M. 
Published: July 2019

Abstract
As an alternative to costly permanent weigh-in-motion (WIM) stations that are mostly limited to major interstate highways, portable WIM systems are often used as a substitute or supplement to routinely collect site-specific traffic data (both volume and weight) for pavement design and analysis applications. By comparison, portable WIM systems are cost effective and much easier to install at any desired highway site/location. However, accuracy, reliability, and data quality have been some of the key challenges of portable WIM systems. As a means of addressing these challenges, this field pilot study was undertaken to comparatively evaluate two different sensor installation methods for routine traffic data measurements: the pocket tape and metal plate methods. The two methods were comparatively evaluated in terms of their practicality, simplicity of installation, cost effectiveness, resource/manpower needs, environmental sensitivity and endurance, consistency, data accuracy, and statistical reliability of the traffic data measurements. Along with a side-by-side field validation using permanent WIM data, the findings from the study indicated that the metal plate sensor installation method is superior to the pocket tape method, particularly in terms of data accuracy, data quality, statistical reliability, and endurance. Its traffic data accuracy rate was found to be 87∼91 % compared with 79 % for the pocket tape method, which exhibited a significant loss of sensitivity and data accuracy after 7 d of traffic measurements. Overall, the conclusions of this study provide technical merit and preference to the metal plate method over the pocket tape sensor installation method, particularly for traffic data measurements exceeding 7 d.
Utilization of Organic Waste and Inorganic Waste in Development of Green Hybrid Composite Material
Authors: Dwivedi Shashi Prakash , Dwivedi Garima 
Published: July 2019

Abstract
Air pollution and soil pollution from organic and inorganic wastes are one of the major problems all over the world. Furthermore, disposal of these organic and inorganic wastes is very costly. In the present investigation, the organic waste considered is rice husk ash (RHA). Rice husk was collected from rice mill industries. The inorganic waste considered is fly ash. Fly ash was collected from thermal power plant industries. By utilizing these wastes in the development of composite materials, some environmental problems can be reduced. In this study, an attempt was made to develop AA6061 base composite material reinforced with different weight percentages of RHA and fly ash. Microstructure of RHA- and fly ash–reinforced composite shows proper distribution of reinforcements. Maximum tensile strength was found to be 145.5 MPa for AA6061 + 5 % RHA + 10 % fly ash composite. Minimum porosity and maximum hardness was found to be 0.80 % and 88 HRB, respectively, for AA6061 +7.5 wt% RHA + 7.5 wt% fly ash hybrid metal matrix composite. In the same way, minimum corrosion loss and minimum thermal expansion were 0.12 mg and 20 mm3 for AA6061 +7.5 wt% RHA + 7.5 wt% fly ash hybrid metal matrix composite, respectively. Minimum water absorption and minimum soil degradation was also found for AA6061 + 7.5 wt% RHA +7.5 wt% fly ash hybrid metal matrix composite. However, density of composites continuously decreases by increasing the percentage of RHA and fly ash in the development of composites.
Combining Transcriptomics and Proteomics Reveals Potential Post-transcriptional Control of Gene Expression After Light Exposure in Metarhizium acridum
Authors: Brancini Guilherme T P , Ferreira Márcia E S , Rangel Drauzio E N , Braga Gilberto Ú L 
Published: July 2019

Abstract
Light is an important stimulus for fungi as it regulates many diverse and important biological processes. Metarhizium acridum is an entomopathogenic fungus currently used for the biological control of insect pests. The success of this approach is heavily dependent on tolerance to environmental stresses. It was previously reported that light exposure increases tolerance to ultraviolet radiation in M. acridum. There is no information in the literature about how light globally influences gene expression in this fungus. We employed a combination of mRNA-Sequencing and high-throughput proteomics to study how light regulates gene expression both transcriptionally and post-transcriptionally. Mycelium was exposed to light for 5 min and changes at the mRNA and protein levels were followed in time-course experiments for two and four hours, respectively. After light exposure, changes in mRNA abundance were observed for as much as 1128 genes or 11.3% of the genome. However, only 57 proteins changed in abundance and at least 347 significant changes at the mRNA level were not translated to the protein level. We observed that light downregulated subunits of the eukaryotic translation initiation factor 3, the eIF5A-activating enzyme deoxyhypusine hydroxylase, and ribosomal proteins. We hypothesize that light is perceived as a stress by the cell that responds to it by reducing translational activity. Overall, our results indicate that light acts both as a signal and a stressor to M. acridum and highlight the importance of measuring protein levels in order to fully understand light responses in fungi.
STUDIES OF WATER FREEZING FEATURES IN ICE CREAM WITH STARCH SYRUP
Authors: Polischuk G. , Sharahmatova T. , Breus N. , Bass O. , Shevchenko I. 
Published: July 2019

The purpose of the study is to research the process of water freezing in new types of ice cream with starch syrup at certain stages of the technological process. Starch syrup as a degradation product of corn starch is characterized by different values of the dextrose equivalent (DE). Starch syrup is a source of solids, sweetener, cryoprotectants (at high DE) and thickener (for low values of DE). The starch syrups with fundamentally different functional and technological properties are chosen for the study: high glucose-fructose syrup HGFS-98 (DE = 98) and low-sugar starch syrup GFS-30 (DE = 30). To determine the size of ice crystals in ice cream, a light microscope of the brand XS-2610 with a cooling chamber is used for an increase of x600, and the cryoscopic temperature is measured by cryostat and Beckmann thermometer (TL-1) to calculate the content of frozen out water. The regularities of the process of water freez-out in ice cream with milk fat content of 3.5%, creamy fat content of 10% and filling with fat content of 15% in the temperature range from minus 6ºС to minus 40ºС are established. In particular, the content of frozenwater in ice-cream at certain stages of the technological process is determined. The results are used to optimize the prescription composition of ice cream with starch syrup. It is recommended to use hydrocarbon complexes consisting of HGFS-98 and GFS-30 in the ratio of 30:70 to 80:20 to reduce the content of frozen water in ice cream of different chemical compositions. The results of the study are of practical importance and allow to obtain in production conditions the fine-crystalline structure of ice cream with starch syrup.
THE FATTY ACIDS CONTENT IN THE LIVER OF JAPANESE QUAILS AFTER THE CHEMICAL TREATMENT OF HATCHING EGGS
Authors: Danchuk V. , Trach V. , Midyk S. , Danchuk О. , Ushkalov V. , Kepple O. 
Published: July 2019

The five groups of Pharaoh quail (100 animals per group) were formed to fulfill the tasks. Quails of the control group were fed by the standard mixed fodder for quail (DSTU 4687:2006). The bird of the research groups (I–IV) received the same feed, but with the addition of 20 g/t of vitamin E. We selected the hatching eggs after 4 weeks of feeding research fodders. Eggs were weighed and laid for incubation using the standard mode after pre-incubation storage of the eggs of the quails obtained in the peak of egg production for 5 days. On the 14th day of incubation, the eggs of the quails were divided into 5 groups. Treatment of eggs I, II and III of the test group was carried out on the 14th day of incubation, respectively, with solutions of 1% sodium hypochlorite 2% perchloric acid 0,5% hydrogen peroxide. Egg of control and IV experimental group were not subject to chemical treatment. The material for research was hatching eggs of quail, liver tissue of 14 daily embryos and 1-day quail. The fatty acid composition of the lipids of tissues and egg yolk was determined in this biological material. Analysis of methyl esters of LC was carried out on a Gas chromatograph Trace GC Ultra (USA) with a flame ionization detector. Identification of fatty acids was carried out using a standard Supelco 37 Component FAME Mix. Quantitative assessment of the spectrum of fatty acids of yolk lipids was carried out by the method of internal normalization, determining their percentage content. Ontogenetic differences in the fatty acid composition of tissues are established. Processing of the eggshell on the fourteenth day of incubation with H2O2 solution is accompanied by an increase in the proportion of stearic acid in the liver of the 1-day quail and a decrease in the proportion of Neukosatrienoic and pre-fatty acids. Treatment of the shell with sodium hypochlorite and HCl is accompanied by an increase in the proportion of stearic acid by 0.96–1.00 % and аrachinic acid, with a decrease in the proportion of gondoinic and eicosatrienic fatty acids. At the same time, when treating with HCl and sodium hypochlorite, the ratio of the sum of saturated to unsaturated fatty acids decreases by 3.2–7.9% (p<0.05). So, the established changes in the fatty acid composition of the liver one-day quail indicate a significant effect of the chemical treatment of the egg shell on the exchange of fatty acids in the embryonic period.
Low Level of Genetic Variation and Signs of Isolation in the Native Hungarian Sea Buckthorn Population Compared to Cultivated Specimens
Authors: HÖHN Mária , SZELÉNYI Magdolna , HALÁSZ Júlia 
Published: July 2019

Sea Buckthorn population from Újpest Nature Reserve, the only one assumed to be native in Hungary sustains at the periphery of Budapest city on the sandy substrate deposited by the Danube river. The study aims to characterize the gene stock preserved on this small edaphic habitat by comparing with the genetic material of planted specimens of known origin used in horticultural and gardening practice. Fragment length variations of seven nuclear microsatellite markers were evaluated and a phylogenetic tree was constructed based on sequence variation of an intron from nuclear gene encoding the chalcone synthase enzyme. Gender rate was evaluated by female specific SCAR marker. Microsatellite analysis pointed out the extremely low variation of the natural sea buckthorn population from Újpest Nature Reserve compared to the cultivated specimens, while the phylogenetic tree revealed that the natural population belongs to the genetic lineage distributed mostly in Western and Middle-Europe, and in Romania, region, which has strong links to the Balkan and the Black Sea. The analysis clearly separated the genetic material of the native Újpest population from the planted individuals from the vicinity that proved to be of allochthonous origin. The HrX2 SCAR marker revealed the dominance of the males (1:1.42). The study provides support for the autochthonous origin of the Hungarian remnant population being in reproductive isolation that suggest for its long persistence and relict status. Ex situ conservation and nature protection measures are highly recommended to maintain one of the last native sea buckthorn populations of the Pannonian region.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Effect of Different DNA Demethylating Agents on In vitro Cultures of Peach Rootstock GF 677
Authors: BARÁNEK Miroslav , OTMAR Miroslav , KREČMEROVÁ Marcela , EICHMEIER Aleš , MOUDRÁ Jana , MYNARZOVÁ Zuzana 
Published: July 2019

The appearance of somaclonal variability induced by in vitro cultivation is relatively frequent and, in some cases, provides a valuable source of new phenotypes suitable for crop improvement. Numerous studies have confirmed that these changes can be explained by alterations of DNA methylation. Interestingly, a group of chemical compounds termed ‘demethylating agents’ (DMT agents) enable artificial changes to be made in the DNA methylation state. Thus, these agents are theoretically able to induce new phenotypes or more favourable properties. The objective of the present study was to verify suitable conditions for the application of different DMT agents within in vitro protocols for micropropagation using the stone fruit rootstock GF 677 as an example. The impact of these agents on the properties of plant regenerants was evaluated, and their DNA methylation state was controlled by using an AFLP protocol based on a restriction endonuclease that differed in its sensitivity to methylated cytosines. Moreover, the effect of newly synthesised derivates was compared with that of conventional compounds with a well-documented DNA-demethylating impact. Based on the results, the suitable concentration for treatment by a DMT agent was established as approximately 50 µM. Promising results were generated using a combination of DMT agents with different mechanisms of action, such as azacytidine and dihydroxypropyladenine; under these conditions, probable synergy between methyltransferase interception by the cytosine analogue and interruption of methyl group donation by dihydroxypropyladenine significantly changed the DNA methylation state of treated plants. Regarding newly synthesised compounds, the 5,6-dihydro-5-azacytosine nucleoside showed the most promising results, which can likely be explained by its higher stability in the media used for in vitro cultivation.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Phytosociology, Distribution and Ecology of a Willow Community with False Tamarisk from the Lotru Valley (Romanian Carpathians)
Authors: NUȚĂ Ilie Silvestru , NICULESCU Mariana 
Published: July 2019

The overall objective of this research was to contribute to a better knowledge of the phytosociology, ecology and distribution of the plant community formed by Myricaria germanica (false tamarisk) and the willow species Salix purpurea. This plant community has an important role in reducing the speed and strength of the water flow during floods. The description was based on cover‐abundance data for the faithful, dominant, characteristic and companion species of this plant community. A predominance of Myricaria germanica was observed when analysing the phytocoenoses, as the species finds in this area favourable ecological conditions for an abundant development. Salix purpurea presents a small abundance-dominance or in some phytocoenoses may even lack. The field research also revealed the excessive development of the species Calamagrostis pseudophragmites in some areas, related mostly with the effect of human activities. The anthropogenic disorders have induced modifications in the floristic physiognomy and compositions of the phytocoenoses, favouring also the invasion of opportunistic species such as Ambrosia artemisiifolia, Impatiens glandulifera, Phytolacca amearicana, Amorpha fruticosa, Erigeron annuus, which continuously extend in the studied territory. As Myricaria germanica and Salix purpurea phytocoenoses suffer a great decline throughout Europe, the habitat built by these species was declared of interest and was included in the Habitats Directive.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Arbuscular Mycorrhiza Improves Leaf Food Quality of Tea Plants
Authors: SHAO Ya-Dong , ZHANG De-Jian , HU Xian-Chun , WU Qiang-Sheng , JIANG Chang-Jun , GAO Xiu-Bing , KUČA Kamil 
Published: July 2019

Tea (Camellia sinensis) plants inhabit arbuscular mycorrhizal fungi (AMF) in rhizosphere, whereas it is not clear whether AMF improves leaf food quality of tea plants. A potted study was conducted to determine effects of Claroideoglomus etunicatum, Diversispora spurca, D. versiformis and a mixture of the three AMF species on leaf sugar, amino acid, soluble protein, tea polyphenol, catechuic acid, and flavonoid contents of Camellia sinensis ‘Fuding Dabaicha’ seedlings. After 12 weeks of AMF inoculation, mycorrhizal plants recorded significantly higher shoot biomass and total leaf area, whilst the effect was ranked as C. etunicatum > D. spurca > mixed-AMF > D. versiformis in the decreasing order. AMF treatments significantly increased leaf total amino acid concentrations, accompanied with up-regulation of amino acid synthetic enzymes genes glutamine synthetase (CsGS), glutamate synthase (CsGOGAT) and glutamate dehydrogenase (CsGDH). Leaf glucose, sucrose, total soluble protein, tea polyphenol, catechuic acid, and flavonoid contents were significantly higher in AMF- than in non-AMF-inoculated plants. In addition, mycorrhizal inoculation notably up-regulated the expression level of leaf 3-hydroxy-3-methylglutaryl coenzyme gene (CsHMGR), ascorbate peroxidase gene (CsAPX), and tea caffeine synthase 1 gene (CsTCS1). These results implied that AMF inoculation had positive effects on leaf food quality partly by means of up-regulation of relevant gene expression in ‘Fuding Dabaicha’ seedlings.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Effects of Different Shading Rates on the Photosynthesis and Corm Weight of Konjac Plant
Authors: QIN Yaoguo , YAN Zesheng , GU Honghui , WANG Zhengxiang , JIANG Xiong , CHEN Zhizhou , YANG Fuqing , YANG Cuiqin 
Published: July 2019

To study the effects of shading level on the photosynthesis and corm weight of konjac plant, the chlorophyll fluorescence parameters, daily variation of relative electron transport rate (rETR), net photosynthetic rate (Pn), and corm weight of konjac plants under different treatments were measured and comparatively analyzed through covered cultivation of biennial seed corms with shade nets at different shading rates (0%, 50%, 70%, and 90%). The results showed that with the increase in shading rate, the maximum photochemical efficiency, potential activity, and non-photochemical quenching of photosystem Ⅱ (PSⅡ) of konjac leaves constantly increased, whereas the actual photosynthetic efficiency, rETR, and photochemical quenching of PSⅡ initially increased and then decreased. This result indicated that moderate shading could enhance the photosynthetic efficiency of konjac leaves. The daily variation of rETR in konjac plants under unshaded treatment showed a bimodal curve, whereas that under shaded treatment displayed a unimodal curve. The rETR of plants with 50% treatment and 70% treatment was gradually higher than that under unshaded treatment around noon. The moderate shading could increase the Pn of konjac leaves. The stomatal conductance and transpiration rate of the leaves under shaded treatment were significantly higher than those of the leaves under unshaded treatment. Shading could promote the growth of plants and increase corm weight. The comprehensive comparison shows that the konjac plants had strong photosynthetic capacity and high yield when the shading rate was 50%-70% for the area.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis
Authors: CAI Xiao Dong , CHENG Jin Jin , ZHANG Yuan Shan 
Published: July 2019

Trichosanthes kirilowii Maxim is a perennial plant possessing great medicinal and edible value. In this study, an efficient rapid propagation system was developed for in vitro production of high-quality plantlets of T. kirilowii cv. ‘Yuelou-2’ via organogenesis. Shoots were established from the nodal stems of this plant by cultured on Murashige and Skoog (MS) basal medium containing different concentrations of naphthalene acetic acid (NAA), indole-3-butyric acid (IBA) and 6-benzyladenine (6-BA) according to a L9 (34) orthogonal array design. MS medium supplemented with 0.5 mg l-1 6-BA alone conferred significant enhancement of shoot induction and development by variance and statistical analysis of shoot induction frequency, shoot length, as well as lateral shoot number and total node number per explant. Rooting percentage, root morphological characteristics, plantlet survival rate, as well as plantlet growth performance in vitro and ex vitro were comprehensively assessed, and results showed that the addition of 1.0 mg l-1 NAA to 1/2 MS medium was the most responsive for root induction and production of high-quality plantlets from the regenerated shoots. The in vitro propagation system described here has the potential for large-scale and rapid production of in vitro plantlets of seed variety of T. kirilowii in a sustainable manner.

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.
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