bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enzymatic activity of palmitoyl‐protein thioesterase‐1 in serum from schizophrenia significantly associates with schizophrenia diagnosis scales
Authors: Wu Yaoyao , Zhang Qianqian , Qi Yawei , Gao Jingjing , Li Wenqiang , Lv Luxiang , Chen Guanjie , Zhang Zhongjian , Yue Xuyi , Peng Shiyong 
Published: July 2019

AbstractGenome‐wide association studies have confirmed that schizophrenia is an inheritable multiple‐gene mental disorder. Longitudinal studies about depression, first episode psychosis (FEP) and acute psychotic relapse have mostly searched for brain imaging biomarkers and inflammatory markers from the blood. However, to the best of our knowledge, the association between enzymatic activities with diagnosis or prediction of treatment response in people with schizophrenia has barely been validated. Under the Longitudinal Study of National Mental Health Work Plan (2015‐2020), we have studied a subsample of approximately 36 individuals from the cohort with data on palmitoyl‐protein thioesterase‐1 enzymatic activity from FEP and performed a bivariate correlation analysis with psychiatric assessment scores. After adjusting for sex, age, body mass index (BMI) and total serum protein, our data demonstrated that PPT1 enzymatic activity is significantly associated with schizophrenia and its Positive and Negative Syndrome Scale (PANSS) scores. This longitudinal study compared the PPT1 enzymatic activity in FEP schizophrenia patients and healthy volunteers, and the former exhibited a significant 1.5‐fold increase in PPT1 enzymatic levels (1.79 mmol/L/h/mL, and 1.18 mmol/L/h/mL; P < 0.05; 95% CI, 2.3‐2.9 and 1.4‐1.8). The higher PPT1 enzymatic levels in FEP schizophrenia patients were positively associated with larger PANSS scaling scores (r = 0.32, P = 0.0079 for positive scaling; r = 0.41, P = 0.0006 for negative scaling; r = 0.45, P = 0.0001 for general scaling; and r = 0.34, P = 0.0048 for PNASS‐S scaling). Higher enzymatic PPT1 in FEP schizophrenia patients is significantly associated with increased PANSS scaling values, indicating more serious rates of developing psychosis. Enzymatic activity of PPT1 may provide an important new view for schizophrenia disorders.
The Akt/FoxO/p27Kip1 axis contributes to the anti‐proliferation of pentoxifylline in hypertrophic scars
Authors: Yang Fangfang , Chen Erfei , Yang Yunshu , Han Fu , Han Shichao , Wu Gaofeng , Zhang Min , Zhang Jian , Han Juntao , Su Linlin , Hu Dahai 
Published: July 2019

AbstractHypertrophic scars (HS) are characterized by the excessive production and deposition of extracellular matrix (ECM) proteins. Pentoxifylline (PTX), a xanthine derived antioxidant, inhibits the proliferation, inflammation and ECM accumulation of HS. In this study, we aimed to explore the effect of PTX on HS and further clarify the mechanism of PTX‐induced anti‐proliferation. We found that PTX could significantly attenuate proliferation of HS fibroblasts and fibrosis in an animal HS model. PTX inhibited the proliferation of HSFs in a dose‐ and time‐dependent manner, and this growth inhibition was mainly mediated by cell cycle arrest. Transcriptome sequencing showed that PTX affects HS formation through the PI3K/Akt/FoxO1 signalling pathway to activate p27Kip1. PTX down‐regulated p‐Akt and up‐regulated p‐FoxO1 in TGF‐β1 stimulated fibroblasts at the protein level, and simultaneously, the expression of p27Kip1 was activated. In a mouse model of HS, PTX treatment resulted in the ordering of collagen fibres. The results revealed that PTX regulates TGFβ1‐induced fibroblast activation and inhibits excessive scar formation. Therefore, PTX is a promising agent for the treatment of HS formation.
Protection against diabetic cardiomyopathy is achieved using a combination of sulforaphane and zinc in type 1 diabetic OVE26 mice
Authors: Wang Jiqun , Wang Shudong , Wang Wanning , Chen Jing , Zhang Zhiguo , Zheng Qi , Liu Quan , Cai Lu 
Published: July 2019

AbstractSulforaphane (SFN) can effectively induce nuclear factor E2–related factor 2 (Nrf2), and zinc (Zn) can effectively induce metallothionein (MT), both of which have been shown to protect against diabetic cardiomyopathy (DCM). However, it is unclear whether combined treatment with SFN and Zn offers better cardiac protection than either one alone. Here, we treated 5‐week‐old OVE mice that spontaneously develop type 1 diabetes with SFN and/or Zn for 18 weeks. Cardiac dysfunction, by echocardiography, and pathological alterations and remodelling, shown by cardiac hypertrophy, fibrosis, inflammation and oxidative damage, examined by histopathology, Western blotting and real‐time PCR, were observed in OVE mice. All these dysfunction and pathological abnormalities seen in OVE mice were attenuated in OVE mice with treatment of either SFN, Zn or SFN/Zn, and the combined treatment with SFN/Zn was better than single treatments at ameliorating DCM. In addition, combined SFN and Zn treatment increased Nrf2 function and MT expression in the heart of OVE mice to a greater extent than SFN or Zn alone. This indicates that the dual activation of Nrf2 and MT by combined treatment with SFN and Zn may be more effective than monotherapy at preventing the development of DCM via complementary, additive mechanisms.
Risk factors for recurrence of periodontal disease in patients in maintenance care in a private practice
Authors: Müller Campanile Véronique , Megally Andrew , Campanile Gaetano , Gayet‐Ageron Angèle , Giannopoulou Catherine , Mombelli Andrea 
Published: July 2019

AbstractAimTo assess periodontal and dental conditions in individuals in maintenance care after periodontal therapy in private practice, and to identify risk factors for recurrence of disease and tooth loss.Materials and MethodsOne hundred patients attending a routine recall visit were included. All had been treated for periodontal disease and were in maintenance since ≥ 2 years.ResultsExaminations took place 18.0 (±8.71) years after the start of periodontal therapy. A total of 40.1 ± 22.5 recall visits were registered during this time. 91% of the participants had an initial diagnosis of chronic, 9% of aggressive periodontitis. The average participant was 46 years old and had 26 teeth. 283 of 2,549 initially present teeth were lost, half of them being molars. Periodontal and endo‐periodontal complications accounted for only 16 lost teeth. The prevalence of all probing depth (PD) categories decreased significantly. The longer the time, the more frequent the recall visits, and the more was spent during the maintenance phase, the greater was the reduction. Multivariate analysis rendered BMI and smoking as factors influencing number of sites with PD ≥ 4 mm and bleeding on probing.ConclusionTooth loss and periodontal tissue damage can be contained over prolonged periods if periodontal disease is treated and patients attend regular maintenance care.
Transmission, relatedness, and the evolution of cooperative symbionts
Authors: Leeks Asher , dos Santos Miguel , West Stuart A. 
Published: July 2019

AbstractCooperative interactions between species, termed mutualisms, play a key role in shaping natural ecosystems, economically important agricultural systems, and in influencing human health. Across different mutualisms, there is significant variation in the benefit that hosts receive from their symbionts. Empirical data suggest that transmission mode can help explain this variation: vertical transmission, where symbionts infect their host's offspring, leads to symbionts that provide greater benefits to their hosts than horizontal transmission, where symbionts leave their host and infect other hosts in the population. However, two different theoretical explanations have been given for this pattern: firstly, vertical transmission aligns the fitness interests of hosts and their symbionts; secondly, vertical transmission leads to increased relatedness between symbionts sharing a host, favouring cooperation between symbionts. We used a combination of analytical models and dynamic simulations to tease these factors apart, in order to compare their separate influences and see how they interact. We found that relatedness between symbionts sharing a host, rather than transmission mode per se, was the most important factor driving symbiont cooperation. Transmission mode mattered mainly because it determined relatedness. We also found evolutionary branching throughout much of our simulation, suggesting that a combination of transmission mode and multiplicity of infections could lead to the stable coexistence of different symbiont strategies.
Intragastric and intracoelomic injection challenge models of tilapia lake virus infection in Nile tilapia (Oreochromis niloticus L.) fingerlings
Authors: Pierezan Felipe , Yun Susan , Surachetpong Win , Soto Esteban 
Published: July 2019

AbstractTo gain a better understanding of the pathogenesis of tilapia lake virus (TiLV) infections in Nile tilapia (Oreochromis niloticus), fingerlings were challenged with a single dose of 1 × 104 TCID50/fish of TiLV utilizing intracoelomic/intraperitoneal (ICch) or intragastric (IGch) routes. Acute mortalities were present in both groups, reaching 70 and 40% in ICch and IGch after 10 days, respectively. Challenged fish presented erratic swimming, lethargy, anorexia, exophthalmia and cutaneous petechiae and ecchymoses. Histological changes in challenged groups included syncytial formation, intracytoplasmic inclusion bodies and multifocal hepatocellular degeneration and necrosis. In addition, multifocal areas of mild proliferation of glial cells and lymphocytic perivascular cuffing were observed in the brain of exposed challenged groups. TiLV RNA was detected in gills and faeces of challenged fish using quantitative reverse transcriptase–PCR, as well as in the tank water holding challenged fish. Moreover, TiLV RNA was detected in scrolls obtained from formalin‐fixed paraffin‐embedded tissue blocks from challenged fish. Results from this study suggest that IG methods represent an additional method to study the pathogenesis of the disease in this species, as it results in infection and diseases as in naturally occurring cases and does not bypass important mucosal immune responses as injectable routes do.
Association between advanced fibrosis in fatty liver disease and overall mortality based on body fat distribution
Authors: Chung Goh Eun , Heo Nam Ju , Kim Donghee , Kwak Min‐Sun , Yim Jeong Yoon , Kim Joo Sung , Ahmed Aijaz 
Published: July 2019

AbstractBackgroundStudies on association between fatty liver disease and overall mortality have yielded conflicting results. We evaluated the impact of fatty liver disease and advanced fibrosis on overall morality with a focus on body size and abdominal fat distribution measured by computed tomography.MethodsWe performed a prospective cohort study including 34 080 subjects (mean age, 51.4 years; 58.6% men) who underwent abdominal ultrasonography and fat computed tomography, from 2007 to 2015. Fatty liver was diagnosed by ultrasonography, and advanced fibrosis was defined as high probability of advanced fibrosis based on three noninvasive methods, aspartate aminotransferase‐to‐platelet ratio index, non‐alcoholic fatty liver disease fibrosis score, and fibrosis‐4 score. Body size was categorized by body mass index into obese (≥ 25 kg/m2) or nonobese (< 25 kg/m2). Multivariate proportional Cox hazard regression analyses were performed.ResultsThe prevalence of fatty liver disease was 37.5%, while the prevalence of advanced fibrosis in fatty liver disease was 1.8%. During a median follow‐up of 87 months (interquartile range, 62–110), 296 deaths occurred. Fatty liver disease was not associated with higher overall mortality (multivariate‐adjusted hazard ratio [HR] 1.02, 95% confidence interval [CI] 0.77–1.34), while increased subcutaneous adiposity was associated with decreased mortality (HR 0.72, 95% CI 0.60–0.88). Advanced fibrosis resulted in a 3.5‐fold increase in overall mortality (adjusted HR 3.52, 95% CI 1.86–6.65), which was more pronounced in the nonobese.ConclusionsWhile fatty liver disease did not impact overall mortality, subcutaneous adiposity was associated with reduced overall mortality. Advanced fibrosis was an independent predictor of increase in overall mortality.
Impact of different exercise training modalities on energy and nutrient intake and food consumption in sedentary middle‐aged adults: a randomised controlled trial
Authors: Jurado‐Fasoli L. , Amaro‐Gahete F. J. , De‐la‐O A. , Castillo M. J. 
Published: July 2019

AbstractBackgroundExercise could influence energy and macronutrient intake, which could have an important role on body composition changes in response to exercise. The present study aimed to investigate the effects of different training modalities in energy and macronutrient intake, and whether changes in energy and macronutrient intake influences changes in body composition in response to different training modalities.MethodsA 12‐week randomised controlled trial was conducted. Eighty middle‐aged sedentary adults were randomised to: (i) a control group; (ii) physical activity recommendation from the World Health Organization; (iii) high‐intensity interval training; and (iv) whole‐body electromyostimulation training. Dietary intake was assessed using the average of three 24‐h recalls.ResultsHigh‐intensity interval training and whole‐body electromyostimulation training groups showed lower fibre intake and higher dietary energy density. Our results showed a negative association was found between changes in energy intake and changes in lean mass index. No association was found between changes in protein intake and changes in lean mass index.ConclusionsIn conclusion, we observed a higher dietary energy density and lower fibre intake in high‐intensity training groups.
A laboratory‐based evaluation of tube blocking and microbial risks associated with one blended enteral feed recipe
Authors: Madden A. M. , Baines S. , Bothwell S. , Chen E. , Goh S. , Jerome L. , Sommariva‐Nagle C. , Szychta M. 
Published: July 2019

AbstractBackgroundConcerns associated with blended enteral feeds include the risk of blocked tubes and microbial contamination, although the available evidence is limited. The present laboratory‐based investigation aimed to examine these risks in a blended feed providing a nutritionally adequate intake for a hypothetical patient.MethodsA one‐blended feed recipe was made using three different methods (professional, jug and stick blenders) and three storage procedures. Feed samples were syringed via 10‐, 12‐ and 14‐French (Fr) enteral feeding tubes and both blockages and the time taken were recorded. Feed samples were diluted, plated on agars, incubated and bacterial colony‐forming units (CFU) counted. After storage at −80 °C, identification was undertaken using 16S rRNA polymerase chain reaction sequencing.ResultsTwo blockages occurred during 27 administrations of feed made using a professional blender, although they were resolved with a water flush. No blockages occurred with the 14‐Fr tube and administration was quicker with wider tubes (P < 0.00001). There was no significant difference between the total bacterial CFU of feeds prepared using different methods (P = 0.771) or stored differently. The genus of bacteria identified included Enterococcus, Bacillus, lactose‐fermenting Enterobacteriaceae, Pseudomonas and Staphylococcus. Pathogens, such as Clostridium spp., Salmonella spp. and Vibrio spp., were not identified by phenotypic tests used. Sequencing identified Escherichia coli, Shigella spp., Streptococcus lutetiensis and Staphylococcus epidermidis.ConclusionsThe present study found no risk of tube blockages when one blended feed recipe made using three methods was delivered via a 14‐Fr tube. There is concern about bacterial contamination, although this was not influenced by the methods of preparation or storage used in the present study.
Pledgeability, Industry Liquidity, and Financing Cycles
Authors: DIAMOND DOUGLAS W. , HU YUNZHI , RAJAN RAGHURAM G. 
Published: July 2019

ABSTRACTWhy do firms choose high debt when they anticipate high valuations, and underperform subsequently? We propose a theory of financing cycles where the importance of creditors’ control rights over cash flows (“pledgeability”) varies with industry liquidity. The market allows firms take on more debt when they anticipate higher future liquidity. However, both high anticipated liquidity and the resulting high debt limit their incentives to enhance pledgeability. This has prolonged adverse effects in a downturn. Because these effects are hard to contract upon, higher anticipated liquidity can also reduce a firm's current access to finance.
‘Rational use of high‐flow therapy in infants with bronchiolitis. What do the latest trials tell us?’ A Paediatric Research in Emergency Departments International Collaborative perspective
Authors: O'Brien Sharon , Craig Simon , Babl Franz E , Borland Meredith L , Oakley Ed , Dalziel Stuart R ,   on behalf of the Paediatric Research in Emergency Departments International Collaborative (PREDICT) Network, Australasia
Published: July 2019

AbstractBronchiolitis is the most common reason for infants to be hospitalised. Over the past decade, the use of high‐flow nasal cannulae (HFNC) therapy has increased markedly and it is now utilised not only in the intensive care unit setting but in general paediatric wards and emergency departments. The aim of this systematic review was to summarise and critique the current evidence‐base for the use of HFNC in infants with bronchiolitis. We searched Ovid Medline, OvidEmbase, PubMed, Cinahl, Cochrane Library, Cochrane Database of Systematic Reviews and Cochrane Central Register of Controlled Trials for systematic reviews and randomised controlled trials of HFNC therapy in infants with bronchiolitis from 1 January 2000 to 27 June 2018. We identified four randomised controlled trials (n = 1891) of HFNC in infants with bronchiolitis: three of these studies involved infants treated in emergency departments and inpatient paediatric wards in Spain, Australia and New Zealand, and one study involved infants treated in paediatric intensive care units in France. The findings of this review suggest that HFNC should be used as a rescue treatment for hypoxaemic infants who have not responded to standard sub nasal oxygen therapy. The use of HFNC for work of breathing in the absence of hypoxaemia, and severe disease, is not currently supported by the evidence, and should only be considered in the context of an appropriate research trial.
Vertical size structure is associated with productivity and species diversity in a short‐stature grassland: Evidence for the importance of height variability within herbaceous communities
Authors: Brown Charlotte , Cahill James F. 
Published: July 2019

AbstractQuestionsLocal size structure is frequently measured in plant populations and forested systems due to its association with population‐ and community‐level processes. In contrast, size structure is rarely examined within herbaceous communities despite evidence for size‐dependent processes in these systems, suggesting an important functional vegetation property may be widely overlooked. Here, we test whether vertical size structure (a) varies within a short‐stature grassland, (b) varies along a productivity gradient, and (c) enhances the understanding of a foundational ecological pattern: the diversity–productivity relationship.LocationRough fescue grassland in Alberta, Canada.MethodsWe measured the heights of individuals within 32 plots and used average height, the Gini coefficient, and a two‐parameter Weibull distribution to estimate community‐level vertical size structure. Linear mixed models were used to test whether size structure parameters were significantly associated with productivity, species richness, and species evenness.ResultsVertical size structure varied significantly among local communities, such that some were dominated by short individuals while others were comprised of a more equal number of taller individuals. With increasing productivity, there was an increase in average plant height and a decrease in height inequality. Decreased height inequality was associated with reduced species richness with evidence that loss was not size‐dependent. The inclusion of vertical size structure parameters increased the explained variance of the diversity–productivity relationship by ~35% while also increasing its parsimony.ConclusionsEven within a short‐stature grassland, there is substantial functional variation in vertical size structure. Productivity is strongly associated with size structure patterns, and the inclusion of height inequalities greatly enhances productivity–diversity relationships, likely because they are the product of assembly mechanisms such as size‐asymmetric competition, assemblage‐level thinning, and niche complementarity. Overall, this demonstrates that vertical size structure has been a missing mechanism in most community assembly theories and models and should be included in the future.
Fungal infection models: Current progress ofex vivomethods
Authors: Maciel Quatrin Priscilla , Flores Dalla Lana Daiane , Andrzejewski Kaminski Taís Fernanda , Meneghello Fuentefria Alexandre 
Published: July 2019

SummaryExperimental alternativeex vivomodels that simulate infectious processesin vivoare of fundamental importance for the evaluation of new drugs, since in some cases, their execution does not depend on the approval of an ethics committee in research. Although studies using alternative infectious models to evaluate the efficacy of antifungal molecules have been increasingly described and reported, there is no critical consensus that establishes the most appropriate ones regarding the type of infection. Numerous studies contemplateex vivoprotocols of fungal infections on nails, corneas, dentinal tubules and skin and reveal counterpoints and concordances not yet finely confronted. In this minireview, we propose a critical analysis of the mainex vivomodels of fungal infections for the evaluation of new antifungal candidates for both topical and systemic use, as opposed to the advantages and disadvantages of the traditional in vivo models employed in preclinical research.
Impatiens maculifera sp. nov. (Balsaminaceae) Yunnan, China
Authors: Xia Chang‐Ying , Sudhindra R. Gadagkar , Zhao Xin‐Lei , Do Truong Van , Zhu Xiang‐Yun , Qin Ying , Deng Hong‐Ping , Yu Sheng‐Xiang 
Published: July 2019

Impatiens (Balsaminaceae) is a notoriously difficult genus to study for reasons that include small, remote and endemic areas of occurrence, and difficulty in obtaining well‐dried herbarium specimens and separating and reconstructing floral parts from available dried specimens. Impatiens has also been notoriously difficult when it comes to phylogenetic resolution at the infrageneric level, but a new system with two subgenera (Impatiens and Clavicarpa) was recently proposed by Yu et al. and it has made it easier to classify newly discovered species.Impatiens maculifera, a new species of Balsaminaceae from Malipo County, Yunnan, China described here, is similar to I. parvisepala S.X. Yu & Y.T. Hou in having racemose inflorescences, four lateral sepals, clavate capsules and ellipsoid seeds, but differs in having leaves narrowly oval or with elliptic–lanceolate blades (versus obovate or obovate–lanceolate), obvious petioles (versus leaves subsessile), racemose inflorescence with 8–12 flowers (versus 6–8 flowers), flowers greenish yellow (versus yellow), lateral united petals and lower sepal with dense red spots (versus red spots absent), and dorsal petal with apparent stalk at base (versus unapparent stalk). Morphological characters and molecular phylogenetic analyses of DNA sequences from both nuclear ribosomal and plastid genes show that the new species differs distinctively from I. parvisepala. Furthermore, I. maculifera is distinguished from other Impatiens species based on morphological, micromorphological and palynological evidence, and molecular data.