bluuebunny/crossref_metadata_2025_split huggingface dataset:
Network construction of aberrantly expressed miRNAs and their target mRNAs in ventricular myocardium with ischemia–reperfusion arrhythmias
Authors: Tang Jian , Gao Hong , Liu Yanqiu , Song Jing , Feng Yurong , Wang Guilong , He Youqin 
Published: July 2019

Abstract
Background: Increasing evidences have verified that microRNAs (miRNAs) play an important role in formation and progression of various cardiac diseases including arrhythmias. Existing research has showed that certain miRNAs exhibit significantly different expressions and effects in arrhythmias. However, the effect of miRNAs in the progression of hypothermic ischemic–reperfusion arrhythmias (RA) and its potential mechanism remains to be further discussed. Methods: By utilizing a model for hypothermic ischemia–reperfusion of rats, miRNAs expression profiles of ventricular myocardium with global hypothermic ischemia–reperfusion and those of ventricular myocardium with hypothermic ischemia–RA were established through high-throughput sequencing. Furthermore, the aberrantly expressed miRNAs in myocardium with and without hypothermic ischemia–RA were screened and verified. By applying RNAhybrid, MiRanda, and TargetScan software, the target genes of these aberrantly expressed miRNAs were predicted. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, the mRNA targets associated with these miRNAs were determined and the miRNA–mRNA interaction during the progression of cardiovascular diseases was explored. The aberrantly expressed miRNAs related to hypothermic ischemia–RA were validated by employing quantitative fluorescence polymerase chain reaction (PCR). Results: Eight significantly aberrantly expressed miRNAs were identified(novel-miR-1 、novel-miR-16、novel-miR-17、novel-miR-19、novel-miR-30、novel-miR-43、rno-miR-122-5p、rno-miR-429), in which six were up-regulated and two were down-regulated. Moreover, target genes and signaling pathways associated with these aberrantly expressed miRNAs were predicted and analyzed. According to miRNA–mRNA interaction network graph, it was revealed that GJA1 gene was considered as the target of novel-miR-17. Conclusions: Aberrantly expressed miRNAs were possibly associated with the formation mechanism of hypothermic ischemia–RA. Specific miRNAs, such as novel-miR-17 and rno-miR-429 are probably new potential targets for further conducting functional study on hypothermic ischemia–RA.
Evaluation of antagonistic actinomycetes isolates as biocontrol agents against wastewater-associated bacteria
Authors: Salah El-Din Mohamed Walaa , Zaki Dina Fouad Ahmed 
Published: July 2019

AbstractIn wastewater treatment plants the antagonistic activities of actinomycetes could be contributing signiﬁcantly in microbe-removing mechanisms, which are a combination of different factors. For this purpose, out of 58 actinomycetes isolates from a wastewater treatment plant in Al-Fayoum Governorate, Egypt, only 36 different morphological isolates were selected for further studies. Although 35 isolates (97.23%) were active against one or more of tested bacteria, WD5 isolate had broad-spectrum antagonistic activity against both Gram-negative and Gram-positive tested bacteria. WD5 ethyl acetate extract recorded the highest antibacterial activity against Staphylococcus aureus MTCC 96 (23 mm) and the lowest antibacterial activity against Pseudomonas aeruginosa MTCC 2453 (11 mm). Gas chromatography-mass spectrometry analysis of extract using available NIST (National Institute of Standards and Technology) library data identified seven bioactive compounds: 2-methylbutyl acetate, 3′,5′-dinitrobenzoic acid, 1-octadecene, tetradecane, dodecane, docosane, and methylamine,N,N-bis(N.-decyl). Many earlier studies mentioned the antibacterial activity of all above seven compounds. The 16S rRNA sequence of WD5 was recorded in GenBank under the accession number MK491056 and confirmed by comparing it with the known 16S rRNA sequences in GenBank as Streptomyces sp. These results indicated antagonistic activity of actinomycetes in the removal of wastewater-associated bacteria.
Stimuli-responsive Carriers for Controlled Intracellular Drug Release
Authors: Sheng Yan , Hu Jiaming , Shi Junfeng , Lee Ly James 
Published: July 2019

Background:
Stimuli-responsive carriers are a class of drug delivery systems which can
change their physicochemical properties and/or structural conformations in response to specific stimuli.
Although passive and active drug targeting has proved to reduce the side effects to normal cells,
controlled intracellular drug release should be included in drug carriers to enhance the bioavailability
of drugs at the disease site.

Methods:
This review focuses on several recent advances in the development of stimuli-responsive
carriers for spatially and temporally controlled release of therapeutic agents in response to intracellular
stimuli, such as pH, redox potential, reactive oxygen species, enzyme and temperature.

Results:
Among the different types of stimuli, pH-responsive carriers have been mostly used to design
intracellular controlled release system. The sharp difference of redox potential between inside and outside
cells is attributed to the high variation in concentration of glutathione. ROS-responsive carriers
are gaining much attention for selective release of therapeutic agents by sensing oxidative conditions at
different levels. The advantages of utilizing enzymes as the trigger of stimuli-responsive carriers include
diverse types of enzymes, high selectivity of enzyme catalyzed reactions and the mild reaction
conditions involved. Abnormal temperature is another unique stimulus and has been widely used to
trigger controlled release of drug in tumor cells.

Conclusion:
Recent developments highlighted in this paper demonstrate that stimuli-responsive carriers
possess great potential as a new platform for controlled intracellular drug release.
Pengembangan Komik Animasi Digital Untuk Simulasi American Service
Authors: Ekawatiningsih Prihastuti , Rinawati Wika , Mustaqim Ilmawan 
Published: July 2019

Penelitian ini bertujuan untuk mengembangkan media komik animasi untuk simulasi pembelajaran melayani makan dan minum (food and beverage service) di restoran, khususnya materi American Service. Penelitian dilakukan untuk merancang alur cerita American Service dalam bentuk komik, khusunya memilih materi American Service yang sesuai, membuat alur cerita dan mengembangan komik American Service. Penelitian ini menggunakan metode penelitian dan pengembangan model 4D (define, design, developt and dissemination). Define dilakukan untuk menetapkan materi pembelajaran yang dapat disimulasikan dalam cerita komik. Desain dibuat bentuk komik dan developt dilakukan untuk menguji dan memperbaiki komik sekaligus menambah animasi. Disseminasi dilakukan dengan media on-line melalui situs internet Jurusan Pendidikan Teknik Boga dan Busana. Subjek penelitian untuk menguji produk ditetapkan 5 pakar dan 40 orang calon pengguna. Metode pengumpulan data pengujian produk menggunakan teknik Delphi, Focus Group Discussion (FGD) dan observasi tampilan komik. Hasil pengujian dianalisis secara deskriptif kuantitatif dan kualitatif. Penelitian menghasilkan rancangan media komik digital American service dengan sistem American Service, menggunakan aplikasi Comicize-thecomics maker dan Photo talks:speech bubbles. Pada tahap ini dapat diidentifikasi materi untuk pengembangan komik berupa American service.
Heart Rate Measures From Wrist-Worn Activity Trackers in a Laboratory and Free-Living Setting: Validation Study
Authors: Müller Andre Matthias , Wang Nan Xin , Yao Jiali , Tan Chuen Seng , Low Ivan Cherh Chiet , Lim Nicole , Tan Jeremy , Tan Agnes , Müller-Riemenschneider Falk 
Published: July 2019

Background
Wrist-worn activity trackers are popular, and an increasing number of these devices are equipped with heart rate (HR) measurement capabilities. However, the validity of HR data obtained from such trackers has not been thoroughly assessed outside the laboratory setting.

Objective
This study aimed to investigate the validity of HR measures of a high-cost consumer-based tracker (Polar A370) and a low-cost tracker (Tempo HR) in the laboratory and free-living settings.

Methods
Participants underwent a laboratory-based cycling protocol while wearing the two trackers and the chest-strapped Polar H10, which acted as criterion. Participants also wore the devices throughout the waking hours of the following day during which they were required to conduct at least one 10-min bout of moderate-to-vigorous physical activity (MVPA) to ensure variability in the HR signal. We extracted 10-second values from all devices and time-matched HR data from the trackers with those from the Polar H10. We calculated intraclass correlation coefficients (ICCs), mean absolute errors, and mean absolute percentage errors (MAPEs) between the criterion and the trackers. We constructed decile plots that compared HR data from Tempo HR and Polar A370 with criterion measures across intensity deciles. We investigated how many HR data points within the MVPA zone (≥64% of maximum HR) were detected by the trackers.

Results
Of the 57 people screened, 55 joined the study (mean age 30.5 [SD 9.8] years). Tempo HR showed moderate agreement and large errors (laboratory: ICC 0.51 and MAPE 13.00%; free-living: ICC 0.71 and MAPE 10.20%). Polar A370 showed moderate-to-strong agreement and small errors (laboratory: ICC 0.73 and MAPE 6.40%; free-living: ICC 0.83 and MAPE 7.10%). Decile plots indicated increasing differences between Tempo HR and the criterion as HRs increased. Such trend was less pronounced when considering the Polar A370 HR data. Tempo HR identified 62.13% (1872/3013) and 54.27% (5717/10,535) of all MVPA time points in the laboratory phase and free-living phase, respectively. Polar A370 detected 81.09% (2273/2803) and 83.55% (9323/11,158) of all MVPA time points in the laboratory phase and free-living phase, respectively.

Conclusions
HR data from the examined wrist-worn trackers were reasonably accurate in both the settings, with the Polar A370 showing stronger agreement with the Polar H10 and smaller errors. Inaccuracies increased with increasing HRs; this was pronounced for Tempo HR.
Concurrent validity and reliability of cephalometric analysis using smartphone apps and computer software
Authors: Livas Christos , Delli Konstantina , Spijkervet Frederik K. L. , Vissink Arjan , Dijkstra Pieter U. 
Published: July 2019

ABSTRACT

Objectives:
To assess the diagnostic accuracy of two smartphone cephalometric analysis apps compared with Viewbox software.

Materials and Methods:
Pretreatment digital lateral cephalograms of 50 consecutive orthodontic patients (20 males, 30 females; mean age, 19.1 years; SD, 11.7) were traced twice using two apps (ie, CephNinja and OneCeph), with Viewbox used as the gold standard computer software program. Seven angular and two linear measurements, originally derived from Steiner cephalometric analysis, were performed.

Results:
Regarding validity, intraclass correlation coefficients (ICCs) ranged from .903–.983 and .786–.978 for OneCeph vs Viewbox and CephNinja vs Viewbox, respectively. The ICC values for intratool reliability ranged from .647–.993. None of the CephNinja measurements was below the recommended cutoff values of ICCs for reliability.

Conclusions:
OneCeph has a high validity compared with Viewbox, while CephNinja is the best alternative to Viewbox regarding reliability. Smartphone apps may have a great potential in supplementing traditional cephalometric analysis.
Effect of Ferrule Thickness on Fracture Resistance of Teeth Restored With a Glass Fiber Post or Cast Post
Authors: Fontana PE , Bohrer TC , Wandscher VF , Valandro LF , Limberger IF , Kaizer OB 
Published: July 2019

SUMMARY

Purpose:
To investigate the influence of ferrule thickness on fracture resistance after mechanical cycling of teeth restored with different intracanal posts.

Methods and Materials:
One hundred twenty bovine incisor teeth were randomized into six study groups, based on the intracanal post used (fiber post or cast post and core) and the presence and thickness of a ferrule (without ferrule, presence of 0.5-mm or 1-mm-thick ferrule, retaining unaltered 2-mm ferrule height). The root posts and the metal crowns were cemented using an adhesive cement. The samples were subjected to mechanical cycling (at 37°C, 45°, 130 N, 2.2 Hz, and 2 × 106 pulses). Afterward, they were subjected to a fracture load test at a speed of 0.5 mm/min and on a 45° slope until failure occurred. The failures were classified as favorable or unfavorable. The fracture resistance data were analyzed with two-way analysis of variance and Tukey test. The chi-square test was used to analyze the pattern of failure.

Results:
When considering the cast post and core, the 1-mm ferrule thickness group presented a higher resistance to fracture than did the group in which a ferrule was not used (p=0.001). When using a glass fiber post, the groups showed no differences in fracture resistance. Overall, 96.7% of the specimens survived the mechanical cycling. Of the fractures, 58.6% of the fractures were unfavorable, while 41.6% were favorable.

Conclusions:
A thicker ferrule statistically increased the fracture resistance only for cast post and core when it was at least 1 mm thick, despite causing more unfavorable failures. Thus, ferrule thickness should be considered when choosing different intracanal posts, to reduce the occurrence of unfavorable failures. In the absence of a ferrule, the use of a cast post and core presents more favorable failures, and in the presence of a 1-mm-thick ferrule, the use of a glass fiber post seems to be the best clinical decision.
RANCANGAN SISTEM HIDROPONIK BUDIDAYA BAWANG MERAH (Allium Ascalonicum L.) DAN SIMULASI ANALISIS BIAYA
Authors: Rachmawaty Annisa Nur , Triyono Sugeng , Suharyatun Siti , Telaumbanua Mareli 
Published: July 2019

Traditional cultivation of shallot is subject to uncertainty both in productivity and price. This seasonal situation was primarily due to climatic factors. Hydroponics cultivation offers a potential solution to that problem because hydroponics was not dependant to climate. Therefore, production can be maintained throughout a year around. This research aims to design hydroponics system for shallot cultivation, to simulate cost analysis, and to estimate profit. The research was conducted by constructing a hydroponics module with dimension as the following: 100 cm high, 3 m long and 60 cm wide. Growth medium made from rice hush char as deep as 15 cm was used in the module. 114 cloves of shallot were nursed, and transplanted to the bed after shoots developed about 5 cm, with 10x15 cm spacing. Parameters observed in this study included pH, EC, moisture content, and plant growth. In addition, three scenarios of the hydroponics systems were simulated to elaborate cost and profit estimation. The three scenarios included scaling up the cultivation beds, ten year cultivation, and productivity from three types of hydroponics modules. The results showed that during hydroponics cultivation of shallot, EC of nutrient solution was elevated to the last level of 3106 μS/cm, while pH was found to be 7.58. The yield of the shallot was 0.0154 kg/m2 with average tuber diameter of 10-15 mm. This production was suboptimal, yet profit and cost comparisons could be clearly described through the simulations of three types of hydroponics modules. Keywords: cost and profit analysis, hydroponics cultivation, nutrition solution, shallot
VAPOR HEAT TREATMENT AND ITS EFFECT ON MELON (Cucumis melo L.) QUALITIES DURING STORAGE
Authors: Hutabarat Michael Alexander , Hasbullah Rokhani , Solahudin Mohamad 
Published: July 2019

Melon is very popular among Indonesians because of its sweet taste and rich in nutrients and also very potential as export comodities. Every export comodities including melon need proper handlings of quarantine to disinfestation pests / diseases. One of quarantine technique which is appliable for melon is vapor heat treatment (VHT). Aims of this research are to make a simulation of heat distribution during VHT process inside melons, to observe VHT technique effects on melon qualities during storage, and to determine the optimum time of VHT process for melon. Finite difference method is used in designing the simulation using Visual Basic 6.0. To observe melon qualities, this research used complete randomized design (CRD) with 4 level of treatment based on VHT process duration which was 10 minutes, 20 minutes, 30 minutes and control every 4 days for 24 days. The result showed that finite difference method can be used for simulating heat distribution inside melon during VHT with coefficient determination (R2) value of 0.9903. Beside that, the result also showed that there were no signifficant difference between each treatments. Based on these results, VHT with 46.5oC temperature and 10 minutes duration time considered as the best treatment.
Manajemen Irigasi Pembibitan Sawit (Elaeis guineensis) Presisi dengan Cropwat 8.0
Authors: Safitri Lisma 
Published: July 2019

The accurate water use information at each stage of plant growth is important to better understand the efficient and precise crop water requirement for optimal plant productivity. Nurseries of palm oil are a phase where young palm oil requires extra maintenance, particularly in meeting the plant water needs. The palm oil in the nursery phase require the regular irrigation schedule due to the vulnerable root systems. The purpose of this study was to calculate the oil palm water requirement with Cropwat 8.0 toward the precise irrigation management and provide a scenario for irrigation scheduling in palm oil nursery. The study was conducted in palm oil main nurseries at KP2 Instiper Yogyakarta with site-specific climate data and soil properties. The method used is analyzing climate data and soil properties and simulating crop water requirements, actual water use and irrigation scheduling with Cropwat 8.0. Based on the results, the average of crop water requirement (ETP) of palm oil in main nursery is 3.4 mm / day. Based on the water deficit scenario from rainfall and crop water requirements, irrigation is scheduling in April for 1.4 mm, May for 18.3 mm, June for 3.5 mm, July for 44.1 mm and August for 42.8 mm. On a daily scale and taking into account the availability of soil moisture and the water retention of plant roots, the net irrigation scheduling is given at an average of 2.2 mm / day and gross irrigation of 6 mm / day which is given daily depending on rainfall and plant age.
QUALITY CHARACTERISTICS OF BEEF HAMBURGER ENRICHED WITH RED GRAPE POMACE POWDER DURING FREEZING STORAGE
Authors: Abdelhakam O , Elsebaie E , Ghazi A , Gouda M 
Published: July 2019

Integrating the consumption of agro-industrial residues with satisfactory consumers is an opposition that, may affect the financial dividend of the frozen meat manufacturing, aside from producing products with diversified nutritional values and satisfying technical characteristics. Thence, we aimed in this research to elaborate and appraise the physicochemical and microbiological characteristics of beef hamburger with the addendum 2 and 4 % of red grape pomace powder (RGPP) throughout its stockpiling at (-18 ± 2°C) over a period 12 weeks. A significant influence on pH value was observed at the beginning (zero time), however the control sample, 2% and 4% had pH values of 6.68, 6.41 and 6.22, respectively. Meantime, there was a significant influence of (RGPP) on thiobarbituric acid (TBA) evidenced on 4th week. The results showed that the microbial growth increased as a function to storage period for all and the addendum of RGPP in beef burger caused a reduction in total bacterial count in comparison with control. Thus, in general, it can be concluded that addendum of RGPP is an efficacious mean to improve burger quality and increasing its storage stability.Key words: beef burger; red grape pomace; storage stability
OCCURRENCE AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM BETA-LACTAMASE PRODUCING Enterobacteriaceae IN MILK AND SOME DAIRY PRODUCTS
Authors: Gaffer Walaa , Gwida Mayada , Abo Samra Randa , Al- Ashmawy Maha 
Published: July 2019

In the present study a total of one hundred samples were randomly purchased from different supermarkets in Damietta city, Egypt, including 25 samples each of raw milk, ice cream, Kareish and Domiati cheese. The collected samples were screened initially for cefotaxime-resistant bacteria using selective enrichment. The suspected colonies were further characterized by analysis of resistance genes in ESBL-producing isolates. A total of 59 ESBLs producers colonies (24 from raw milk, 15 from kareish cheese, 13 from Domiati cheese and 7 from ice cream) were recovered and identified as Escherichia coli (n = 29), Klebsiella pneumonia (n = 8), Klebsiella oxytoca (n = 5), Enterobacter aerogenes (n =8), Citrobacter diversus (n = 8) and Serratia liquefaciens (n = 1). Resistance to two or more antibiotics was observed among the recovered isolates. E. coli isolates showed high resistance pattern against cefaclor (100%), cefoxitin (100%), cefalaxim (96.5%), ceftazidime (93%), pencillin (93%) and 79% for Pipercillin/Tazobactam. All the recovered Klebseilla spp. isolates were resistant to Cefepime and Pipercillin/Tazobactam (84.6% each) and exhibited 100% resistant to other antimicrobials agents. Meanwhile, all the recovered isolates were sensitive to imipenem and meropenem. Gene encoding blaCTX-M1 was mostly predominant among screened genes, being present in 13 (48%) dairy samples for blaTEM and blaSHV, they were detected in 12 (44%) and 4 (14.8%) isolates, respectively. In conclusion, there is clear evidence of circulation of antibiotic-resistant food borne ESBL producing Enterobacteriaceae in the examined dairy samples. The concern about increasing the risk of dissemination of such multi-drug resistant pathogens rises with a potential asymptomatic colonization and complication of systemic infection in human subjects. There is also a possible interface for the exchange of resistance genes within and across species and with commensal bacteria of the human and animals.Key words: dairy products; ESBL producing Enterobacteriaceae; Egypt; public health
IMPACT OF L-CARNITINE SUPPLEMENTATION ON GROWTH OF BROILER CHICKEN THROUGH DETERMINATION OF CHANGES IN THE EXPRESSION OF CAT2, MYOD AND MYF5 GENES
Authors: Abouzed Tarek , Dorghamm Doaa , Kahilo Khaled , Elkattawy Azza , Nassef Eldsokey , El-sawy Hanan 
Published: July 2019

This study explored the impact of supplementation of L- carnitine on the performance of broiler chicken, biochemical parameters and expression of cationic amino acid transporter (CAT2), myogenic determining factor (MYOD) and myogenic factor 5 (MYF5) genes. This study included two groups; the first one is the control group and the second one received L-carnitine at a dose 50 mg/kg/day in drinking water. After 35 day, the sera and muscle tissue were obtained for biochemical and real time polymerase chain reaction (qPCR) analysis. The obtained results revealed a significant increase in the body weight, total body weight gain and a significant decrease in food consumption, food conversion rate in L-carnitine-supplemented group as compared to the control group. Moreover, there was no significant changes in serum cholesterol, triacylglycerol, AST, ALT, total protein and uric acid levels which showed a slight decrease in L-carnitine-supplemented group in comparison with the control group. This study also revealed a significant up regulation for the expression of CAT2, MYOD and MYF5 genes in muscles of broiler chicken-supplemented with L- carnitine as compared to the control one. The present study concluded that supplementation of L- carnitine to broiler chicken was useful to increase the total body weight gain and muscle mass and this beneficial effect was associated with up regulation of CAT2, MYOD, and MYF5 genes.Key words: L-carnitine; broiler chicken; growth traits; CAT2; MYOD; MYF5