bluuebunny/crossref_metadata_2025_split huggingface dataset:
High‐Throughput Single‐Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC)
Authors: Meteling Marieke , Johnbosco Castro , Wolfel Alexis , Conceição Francisco , Govindaraj Kannan , Moreira Teixeira Liliana , Leijten Jeroen 
Published: December 2024

AbstractExtracellular matrix (ECM) guides cell behavior and tissue fate. Cell populations are notoriously heterogeneous leading to large variations in cell behavior at the single‐cell level. Although insights into population heterogeneity are valuable for fundamental biology, regenerative medicine, and drug testing, current ECM analysis techniques only provide either averaged population‐level data or single‐cell data from a limited number of cells. Here, extracellular protein identification cytometry (EPIC) is presented as a novel platform technology that enables high‐throughput measurements of local nascent protein deposition at single‐cell level. Specifically, human primary chondrocytes are microfluidically encapsulated in enzymatically crosslinked microgels of 16 picoliter at kHz rates, forming large libraries of discrete 3D single‐cell microniches in which ECM can be deposited. ECM proteins are labeled using fluorescence immunostaining to allow for nondestructive analysis via flow cytometry. This approach reveals population heterogeneity in matrix deposition at unprecedented throughput, allowing for the identification and fluorescent activated cell sorting‐mediated isolation of cellular subpopulations. Additionally, it is demonstrated that inclusion of a second cell into microgels allows for studying the effect of cell‐cell contact on matrix deposition. In summary, EPIC enables high‐throughput single‐cell analysis of nascent proteins in 3D microenvironments, which is anticipated to advance fundamental knowledge and tissue engineering applications.
Hypoxia Induced Lnc191 Upregulation Dictates the Progression of Esophageal Squamous Cell Carcinoma by Activating GRP78/ERK Pathway
Authors: Wei Sisi , Fan Xinyi , Li Xiaoya , Zhou Wei , Zhang Zhihua , Dai Suli , Lv Huilai , Liu Yueping , Shan Baoen , Zhao Lianmei , Zhan Qimin , Song Yongmei 
Published: December 2024

AbstractHypoxia is a typical hallmark of solid tumors and plays a crucial role in the progression of esophageal squamous cell carcinogenesis (ESCC). Nevertheless, the precise mechanisms underlying the involvement of hypoxia in tumor development remain unclear. In the present study, a novel hypoxia‐induced long noncoding RNA (lncRNA) is identified first, lnc191, which is highly expressed in clinical ESCC tissues and is positively correlated with poor prognosis of ESCC patients. These findings provide evidence that the hypoxia‐inducible factor‐1α (HIF‐1α)‐mediated transcriptional activation of lnc191 enhances the growth and metastasis of ESCC cells both in vitro and in vivo. Mechanistically, lnc191 interacts with GRP78 (78‐kDa glucose‐regulated protein), one of the endoplasmic reticulum chaperone proteins, leading to its translocation to the membrane, where GRP78 binds with EGFR and enhances its phosphorylation (Y845), further activates ERK/MAPK signaling pathway, and thereby in favor of the progression of ESCC. Overall, this data proposes lnc191 as a key driver during the development of ESCC and reveals the participation of the activated GRP78/ERK/MAPK axis in the ESCC progression mediated by lnc191. These findings indicate the potential of lnc191 as a promising diagnostic biomarker and therapeutic target in ESCC.
Unraveling the Role of the Tumor Extracellular Matrix to Inform Nanoparticle Design for Nanomedicine
Authors: Cassani Marco , Fernandes Soraia , Pagliari Stefania , Cavalieri Francesca , Caruso Frank , Forte Giancarlo 
Published: December 2024

AbstractThe extracellular matrix (ECM)—and its mechanobiology—regulates key cellular functions that drive tumor growth and development. Accordingly, mechanotherapy is emerging as an effective approach to treat fibrotic diseases such as cancer. Through restoring the ECM to healthy‐like conditions, this treatment aims to improve tissue perfusion, facilitating the delivery of chemotherapies. In particular, the manipulation of ECM is gaining interest as a valuable strategy for developing innovative treatments based on nanoparticles (NPs). However, further progress is required; for instance, it is known that the presence of a dense ECM, which hampers the penetration of NPs, primarily impacts the efficacy of nanomedicines. Furthermore, most 2D in vitro studies fail to recapitulate the physiological deposition of matrix components. To address these issues, a comprehensive understanding of the interactions between the ECM and NPs is needed. This review focuses on the main features of the ECM and its complex interplay with NPs. Recent advances in mechanotherapy are discussed and insights are offered into how its combination with nanomedicine can help improve nanomaterials design and advance their clinical translation.
Synergetic Phase Modulation and N‐Doping of MoS2 for Highly Sensitive Flexible NO2 Sensors
Authors: Kim Jiyun , Li Mengyao , Lin Chun‐Ho , Hu Long , Wan Tao , Saeed Ayad , Guan Peiyuan , Feng Zijian , Kumeria Tushar , Tang Jianbo , Su Dawei , Wu Tom , Chu Dewei 
Published: December 2024

AbstractMolybdenum disulfide (MoS2) is a promising electronic material owing to its excellent electrochemical features, high carrier mobility at room temperature, and widely tunable electronic properties. Here, through precursor engineering and post‐treatments to tailor their phase and doping, electronic characteristics of MoS2 are significantly modified. It is found that 2H semiconductor phase with nitrogen doping (N‐doping) in flexible gas sensors constructed with Ag electrodes exhibits the highest sensitivity of ≈2500% toward 10 ppm of NO2. This sensitivity is ≈17‐ and 417‐folds higher than that of 2H MoS2 without N‐doping, and mixed phases with metallic 1T and semiconductor 2H phase, respectively. Comprehensive experimental investigations reveal mechanisms underlying this record sensitivity, that is, the use of N‐doped 2H MoS2 sensors not only significantly suppresses dark current but also effectively enhances electron transfer to NO2 molecules. Moreover, density function theory calculations underpin the experimental results, confirming that N2H4 molecules from the precursor solution not only promote phase transition but also enable N‐doping during post‐treatments, thus boosting sensing capability. This work, for the first time, reveals the synergistic effect of phase modulation and N‐doping of MoS2, which can be readily used in other flexible electronic applications, advancing MoS2‐based electronics to a new stage.
Popcorn Effect–inspired Self‐propagating Formation of High‐conductivity Cement Composite for Multifunctional Applications
Authors: Wu Haiping , Huang Jiaqi , Qu Zhengyao , Zheng Xueling , Tan Sirui , Du Wei , Cai Guanming , Zhao Zhong , Wu Jing , Li Daiqi 
Published: December 2024

AbstractThe surge in modern civil technologies demands a transformation in cement composites to surpass traditional roles and integrate smart functionalities. In this regard, enhancing the electrical conductivity of cement composites is a critical challenge. This study introduces a novel strategy for the self‐propagating formation of expandable graphite‐based high‐conductivity cement composites through a simple thermal treatment with 3 wt.% expandable graphite and 1 wt.%carbon fiber as conductive fillers. Inspired by the popcorn effect, this method leverages the rapid expansion of graphite at high temperatures, promoting contact between conductive fillers and forming new conductive networks. The obtained composites demonstrate a remarkable reduction of 60% in electrical resistance after heat treatment compared to the electrical resistance of standard cement composites, and the enhancing mechanisms is explored. The conductive properties endow the material with excellent electrothermal (>100 °C at 10 V), electrothermochromic (response time of 2 s), and electromagnetic interference shielding (42 dB at 12.4 GHz) performance. This innovative approach provides vast opportunities for developing smart infrastructure with enhanced electrical properties, regarded as a promising candidate for promoting next‐generation buildings and infrastructures.
Water‐Triggered Reconfigurable Glycerogels for Sustainable All‐Gel Supercapacitors
Authors: Mredha Md. Tariful Islam , Rama Varma Adith Varma , Gupta Tanish , Jeon Insu 
Published: December 2024

AbstractThe polymeric structures of synthetic gels are typically static, which makes them damage‐prone and nonrecyclable. Inspired by the dynamic reconfigurability of biological tissues, which eliminate old/damaged cells and regenerate new ones via biological triggers/signals, a reconfigurable biopolymer gel is presented based on a glycerol‐mediated supramolecular gelation strategy. In response to an eco‐friendly triggering agent water, this gel undergoes on‐demand molecular‐level reconfiguration. The versatility of the approach enables the development of reconfigurable gels with modulated functionality. As a proof‐of‐concept, a reconfigurable glycerogel electrode and electrolyte are developed and used to prototype an all‐gel supercapacitor that exhibits exceptional self‐healing, degradation, and rebuilding abilities. Furthermore, it can tolerate extreme mechanical deformations (e.g., stretching, bending, and twisting) and temperatures (−20 to 80 °C). The device exhibits excellent energy storage performance, with a maximum areal capacitance of 450 mF cm−2 (at 0.035 mA cm−2) and remarkable capacitance retention of 89% following 20 000 charge/discharge cycles (at 0.35 mA cm−2). Moreover, following self‐healing and rebuilding, the capacitance remains at 91% and 110% (at 0.35 mA cm−2) of the original value, respectively. This generalized strategy for preparing multifunctional reconfigurable gels will facilitate the development of high‐performance flexible and wearable devices with improved durability and recyclability.
Metal‐Mediated Chlorine Transfer for Molten Salt‐Driven Thermodynamic Change on Silicon Production
Authors: Je Minjun , Kim Jin Chul , Kim Jiyeon , Kim Sungho , Ryu Sunmin , Ryu Jaegeon , Kwak Sang Kyu , Park Soojin 
Published: December 2024

AbstractThe development of silicon (Si) material poses a great challenge with profound technological advancements for semiconductors, photo/photoelectric systems, solar cells, and secondary batteries. Typically, Si production involves the thermochemical reduction of silicon oxides, where chloride salt additives help properly revamp the reaction mechanism. Herein, we unravel the chemical principles of molten AlCl3 salt in metallothermic reduction. Above its melting temperature (Tm ≈ 192 °C), three AlCl3 molecules coordinate with each metal (M) atom (e.g., conventional Al and Mg, or even thermodynamically unfeasible Zn) to form metal‐AlCl3 complexes, M(AlCl3)3. In the molten AlCl3 salt media, all complexes directly lead to the universal formation of AlOCl byproduct and as‐reduced Si spheres through internal Cl* transfer during the reduction reaction. Intriguingly, highly oxophilic metal (i.e., Mg) establishes additional energetic shortcuts in reaction pathways, where AlCl3 directly detaches an oxygen atom, accompanied by strong metal‐oxygen interactions and Cl* transfer within the same complex. Moreover, the thermodynamic stability of the metal‐AlCl3 complex residue (MAl2Cl8) and the microstructure of post‐treated Si do change according to the metal choice, imparting disparate physicochemical properties for Si. This work offers insights into the scalable production of tailored Si materials for industrial applications, along with cost‐effective operations at 250 °C.
High Catalytic Selectivity of Electron/Proton Dual‐Conductive Sulfonated Polyaniline Micropore Encased IrO2 Electrocatalyst by Screening Effect for Oxygen Evolution of Seawater Electrolysis
Authors: Shen Yuhan , Zhao Shengqiu , Wu Fanglin , Zhang Hao , Zhu Liyan , Wu Mingjuan , Tian Tian , Tang Haolin 
Published: December 2024

AbstractAcidic seawater electrolysis offers significant advantages in high efficiency and sustainable hydrogen production. However, in situ electrolysis of acidic seawater remains a challenge. Herein, a stable and efficient catalyst (SPTTPAB/IrO2) is developed by coating iridium oxide (IrO2) with a microporous conjugated organic framework functionalized with sulfonate groups (‐SO3H) to tackle these challenges. The SPTTPAB/IrO2 presents a ‐SO3H concentration of 5.62 × 10−4 mol g−1 and micropore below 2 nm numbering 1.026 × 1016 g−1. Molecular dynamics simulations demonstrate that the conjugated organic framework blocked 98.62% of Cl− in seawater from reaching the catalyst. This structure combines electron conductivity from the organic framework and proton conductivity from ‐SO3H, weakens the Cl− adsorption, and suppresses metal‐chlorine coupling, thus enhancing the catalytic activity and selectivity. As a result, the overpotential for the oxygen evolution reaction (OER) is only 283 mV@10 mA cm−2, with a Tafel slope of 16.33 mV dec−1, which reduces 13.8% and 37.8% compared to commercial IrO2, respectively. Impressively, SPTTPAB/IrO2 exhibits outstanding seawater electrolysis performance, with a 35.3% improvement over IrO2 to 69 mA cm−2@1.9 V, while the degradation rate (0.018 mA h−1) is only 24.6% of IrO2. This study offers an innovative solution for designing high‐performance seawater electrolysis electrocatalysts.
Microwave Ablation Combined with Flt3L Provokes Tumor‐Specific Memory CD8+ T Cells‐Mediated Antitumor Immunity in Response to PD‐1 Blockade
Authors: Wang Meixiang , Sang Jing , Xu Fengkuo , Wang Shulong , Liu Peng , Ma Ji , Chen Zhengtao , Xie Qi , Wei Zhigang , Ye Xin 
Published: December 2024

AbstractFor medically inoperable non‐small cell lung cancer, microwave ablation (MWA) represents a super minimally invasive alternative treatment. However, tumor recurrence remains a concern. Here, it is demonstrated that the combination of MWA with Flt3L significantly inhibits tumor recurrence by CD8+ central memory T (TCM)‐like cell‐dependent antitumor immune responses within the tumor‐draining lymph nodes (TdLN). TdLN‐TCM‐like cells encompassed both tumor‐specific memory T (TTSM) and progenitor‐exhausted T (TPEX) cells. The expansion of these cells markedly altered the differentiation of exhausted T cells within the tumor microenvironment (TME). TPEX predominantly differentiated into transitory effector‐like exhausted T cells (TEX‐int). The expansion of TTSM cells elicited by the combined therapy was reliant on conventional dendritic cells (cDCs) and was likely specifically dependent on the migratory cDC1s (Mig cDC1s) within the TdLN. The upregulation of ICOSL on migratory cDC1s was pivotal in initiating TTSM‐like cell‐mediated antitumor responses. Slc38a2 may be a critical gene responsible for the upregulation of ICOSL in Mig cDC1s following combined treatment. Finally, the combined treatment significantly enhanced the antitumor efficacy of immunotherapy based on PD‐1 blockade. The research thereby afforded a novel strategic approach to forestall tumor recurrence after MWA therapy, while also providing the foundational proof‐of‐concept for impending clinical investigations.
Off‐Flavour Removal With Advanced Oxidation Process and Hydrogen Peroxide Treatments in Recirculating Aquaculture Systems
Authors: Pettersson Samu , Lindholm‐Lehto Petra C. , Pulkkinen Jani T. , Tuhkanen Tuula 
Published: December 2024

ABSTRACTAquaculture is becoming increasingly important for the world's food production. Recirculating aquaculture system (RAS) has a reduced water requirement and better possibilities for waste handling. Unfortunately, off‐flavours can be formed in RAS and concentrate on fish flesh. Off‐flavour compounds cause earthy, musty or other unwanted flavours to fish flesh that consumers find objectionable. Typically, off‐flavours are removed by depurating the fish in clean water, but it often takes from days to weeks to fully remove these unwanted flavours that causes additional costs to fish producers. Therefore, reliable methods to reduce the need for depuration are needed. In this study, two methods were investigated for the removal of off‐flavours in RAS rearing rainbow trout Oncorhynchus mykiss: an advanced oxidation process (AOP) using a combination of ozone (O3) and hydrogen peroxide (H2O2), and a treatment with H2O2 alone. Two treatments (AOP and H2O2) and a control without oxidants were applied across nine identical experimental RASs for 8 h day−1 over 10 days, and selected off‐flavour compounds in water and fish were analysed. In fish, the concentrations of GSM and MIB were on average 776 and 962 ng kg−1 (AOP) and 688 and 919 ng kg−1 (H2O2) compared to 1071 and 1205 ng kg−1 in the controls. The results showed that intensive oxidant treatments reduced the off‐flavour concentrations in the recirculating water and in fish, which can potentially lead to reduced depuration time and production costs. Further optimization of the treatment is needed to improve off‐flavour removal efficiencies.
Using red–green–blue vegetation indices to evaluate complex agronomical traits in soybean breeding
Authors: Vianna Mariana Silva , Matias Filipe Inácio , Galli Giovanni , Martins Emanoel Sanches , Oliveira Maiara , Pinheiro José Baldin 
Published: December 2024

AbstractHigh‐throughput phenotyping is an emerging tool that allows access to identify simple and complex traits, accelerating genetic discoveries and selection. Vegetation indices strongly correlate with several economic crop traits, allowing plant breeders to detect variation in breeding populations. Thus, this study used red–green–blue (RGB) vegetation indices to evaluate the influence of the stink bug complex (Euschistus heros, Piezodorus guildinii, Nezara viridula, Dichelops melacanthus, and Edessa meditabunda) on the agronomical traits of soybean (Glycine max) lineages. For instance, two experiments were conducted to assess soybean resistance to the stink bug complex, (1) with and (2) without pesticide control. An unmanned aerial vehicle coupled with an RGB camera acquired aerial photography over the field during the R5 stage. Four vegetation indices and canopy were estimated from the orthomosaic, and the genotypes were evaluated based on agronomical traits. Linear mixed models were used to estimate the variance and significance test of each trait using the likelihood ratio test, and the principal component analysis was performed to verify the multivariate pattern among genotypes. The results showed significant genotypic effects for most traits with high broad‐sense heritability for agronomical traits and moderate for vegetation indices. Significant correlations using best linear unbiased predictions were observed among the agronomical traits with the vegetation indices and canopy coverage, which can be used as a tool for the indirect selection of soybean lineages in the breeding pipeline.
Risk factors for postoperative complications in laparoscopic and robot‐assisted surgery for octogenarians with colorectal cancer: A multicenter retrospective study
Authors: Yamamoto Takehito , Hida Koya , Goto Kentaro , Fukuda Meiki , Inamoto Susumu , Hashida Hiroki , Matsusue Ryo , Takahashi Ryo , Mizuno Rei , Terajima Hiroaki , Obama Kazutaka 
Published: December 2024

AbstractAimMinimally invasive surgery for colorectal cancer is increasing globally. However, the safety in older patients have not been thoroughly examined.MethodsPatients with colorectal cancer who underwent laparoscopic or robot‐assisted surgery at Kyoto University Hospital and 18 affiliated institutions in Japan that participated in the Kyoto Colorectal Surgery Group between 2018 and 2023 were enrolled. Focusing on patients ≥80 y, we investigated the risk factors for postoperative complications.ResultsIn total, 7303 patients were enrolled in this study. The mean age was 71 ± 11 y, with 1665 patients (22.8%) ≥80 y old. Older patients (≥80 y) had significantly higher ASA and ECOG‐PS scores and more comorbidities including diabetes, chronic obstructive pulmonary disease (COPD), hypertension, heart disease, and cerebrovascular disease than patients ≤79 y old (all p < 0.05). In the older group, postoperative complications (Clavien–Dindo grade ≥II) occurred in 210 patients (12.6%). After adjusting for covariates using the multivariable logistic regression model, rectal cancer (odds ratio [OR]: 1.84, 95% confidence interval [CI]: 1.30–2.60, p = 0.001), operation time ≥300 min (OR: 1.52, 95% CI: 1.07–2.16, p = 0.020), and blood loss ≥100 mL (OR: 2.19, 95% CI: 1.80–3.24, p < 0.001) were associated with the occurrence of complications, whereas their comorbidities showed no association.ConclusionIn minimally invasive colorectal cancer surgery for older patients (≥80 y old), prioritizing shorter operation time and blood loss control is crucial, especially for patients with rectal cancer because of their high risk of complications.
Visible‐Light Driven Photocatalytic Strategies for the Synthesis of Pyrrolines
Authors: Kesharwani Nitya , Garima Km , Srivastava Vishal , Singh Praveen P. , Singh Pravin K. 
Published: December 2024

AbstractPyrroline is a five‐membered nitrogen‐containing heterocyclic compound found in various biologically active compounds, pharmaceuticals, and agrochemicals. Several synthetic methods have been reported for the preparation of these heterocycles, along with their notable biological activities. Visible light and photoredox catalysis have become powerful and enduring tools in organic synthesis. This review highlights the impact of visible light‐driven chemistry on the synthesis and modification of heterocyclic compounds containing pyrroline ring for their medicinal importance.
TRIB3 suppresses milk fatty acids metabolism by inhibiting p‐AKT/PPARG signaling in goat mammary epithelial cells
Authors: Gong Xinglong , Pan Tan , Xiong Tiantian , Zhu Yifan , Loor Juan J. , Han Chengming , Li Yifan , Lei Huan , Luo Jun , Li Cong 
Published: December 2024

AbstractTribbles pseudokinase 3 (TRIB3) interacts with a variety of proteins and plays a key role in the regulation of glucose metabolism and glycolysis in nonruminants, but whether it has a specific role in goat mammary lipid metabolism has still been kept unknown. In this study, we observed that TRIB3 is highly expressed in the mammary tissues of lactating dairy goats. Overexpressing TRIB3 in goat mammary epithelial cells (GMECs) suppressed the mRNA expression of GPAM, DGAT1, and PLIN1, which are associated with the formation of triacylglycerol and lipid droplets (p < 0.05). The fatty acid‐sensitive transcription regulator PPARG was also downregulated. Interfering TRIB3 had the opposite effect and decreased Akt phosphorylation. The TRIB3 gene influenced fatty acid composition in GMECs, and its overexpression reduced the total concentration of intracellular triacylglycerol (p < 0.01), this response was verified using BODIPY staining. Overall, these data indicated that TRIB3 suppresses milk fatty acids metabolism through inhibiting p‐AKT/PPARG signaling in GMECs.