bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heart Rate Variability During Nocturnal Sleep and Daytime Naps in Patients With Narcolepsy Type 1 and Type 2
Authors: Aslan Sabina , Erbil Nurhan , Tezer F. Irsel 
Published: November 2018

Purpose:
Heart rate variability is a noninvasive measure of autonomic function. The aim of this study was to determine the risk of cardiovascular autonomic dysfunction during sleep in patients with narcolepsy. The effect of daytime napping was also examined in patients with narcolepsy type 1 and type 2.

Methods:
Polysomnography recordings and multiple sleep latency tests from 11 patients with narcolepsy type 1 (N1) and 20 patients with narcolepsy type 2 (N2) were included and compared with 12 healthy controls. Heart rate variability values (measured by time- and frequency-domain parameters) were calculated using electrocardiography data from the polysomnography–multiple sleep latency test recordings.

Results:
As an indicator of increasing sympathovagal imbalance, the low-frequency/high-frequency ratio was higher in all patients than in controls during non–rapid eye movement (NREM)-2 stage sleep (P ≤ 0.01). The root mean square of successive RR interval differences was lower (indicating parasympathetic tone) in N1 patients compared with N2 patients during REM sleep (P ≤ 0.04). In addition, decreased heart rate variability values were observed during sleep-onset REM–negative multiple sleep latency test periods compared with sleep-onset REM–positive periods.

Conclusions:
Heart rate variability abnormalities during sleep and the role of these changes on the development of cardiovascular diseases must be investigated in prospective follow-up studies of patients with narcolepsy. Heart rate variability changes during night sleep, daytime napping, and presence of sleep-onset REM periods may affect the life-threatening events.
Beyond a ribosomal RNA methyltransferase, the wider role of MraW in DNA methylation, motility and colonization inEscherichia coliO157:H7
Authors: Xu Xuefang , Zhang Heng , Huang Ying , Zhang Yuan , Wang Xiaoyuan , Wang Dai , Pu Ji , Zhao Hongqing , Lu Xuancheng , Lu Shuangshuang , Xiong Yanwen , Ye Changyun , Dong Yuhui , Lan Ruiting , Xu Jianguo 
Published: November 2018

ABSTRACTMraW (RsmH) is an AdoMet-dependent 16S rRNA methyltransferase conserved in bacteria and plays a role in the fine-tuning of the ribosomal decoding center. It was recently found to contribute to the virulence ofStaphylococcus aureusin host animals. In this study, we examined the function of MraW inEscherichia coliO157:H7 and found that deletion ofmraWled to decreased motility and flagellar production. Whole-genome bisulfite sequencing showed genome wide decrease of methylation of 336 genes and 219 promoters in themraWmutant. The methylation level of 4 flagellar gene sequences were further confirmed by bisulfite PCR sequencing. Quantitative reverse transcription PCR results indicated the transcription of these genes was also affected. MraW was observed to directly bind to the four flagellar gene sequences by electrophoretic mobility shift assay (EMSA). A common motif in differentially methylated regions of promoters and coding regions of the 4 flagellar genes was identified. Reduced methylation was correlated with altered expression of 21 of the 24 genes tested. DNA methylation activity of MraW was confirmed by DNA methyltransferase (DNMT) activity assayin vitro. ThemraWmutant colonized poorer than wild type in mice. we further found that the expression ofmraZin themraWmutant was increased confirming the antagonistic effect ofmraWonmraZ. In conclusion,mraWwas found to be a DNA methylase and has a wide-ranging effect onE.coliO157:H7 including motility and virulencein vivovia genome wide methylation andmraZantagonism.IMPORTANCEMraW is a well-studied 16S rRNA methyltransferase and was recently found have an impact on bacterial virulence. Here we demonstrated its new function as a DNA methylase and effect on motility, colonization in mice, DNA methylation in genome wide and contribution to virulence. Its direct binding of differentially methylated flagellar-encoding DNA sequences was observed, indicating a correlation between DNA methylation and regulation of flagellar genes. In addition, the expression ofmraZwhich function as an antagonist ofmraWwas increased in themraWmutant.mraWplays an important role in gene regulation likely through DNA methylation. Clearly it plays a role in virulence inE. coliO157:H7. It also opens a new research field for virulence study in bacteria.
Very rapid flow cytometric assessment of antimicrobial susceptibility during the apparent lag phase of microbial (re)growth
Authors: Jindal Srijan , Thampy Harish , Day Philip J. , Kell Douglas B. 
Published: November 2018

AbstractCells ofE. coliwere grown in LB medium, taken from a stationary phase of 2-4h, and reinoculated into fresh media at a concentration (105.mL-1or lower) characteristic of bacteriuria. Flow cytometry was used to assess how quickly we could detect changes in cell size, number, membrane energisation (using a carbocyanine dye) and DNA distribution. It turned out that while the lag phase observable macroscopically via bulk OD measurements could be as long as 4h, the true lag phase could be less than 15-20 min, and was accompanied by many observable biochemical changes. Antibiotics to which the cells were sensitive affected these changes within 20 min of reinoculation, providing the possibility of a very rapid antibiotic susceptibility test, on a timescale compatible with a visit to a GP clinic. The strategy was applied successfully to genuine potential Urinary Tract Infection (UTI) samples taken from a doctor’s surgery. The methods developed could prove of considerable value in ensuring the correct prescription and thereby lowering the spread of antimicrobial resistance.
TET-catalyzed 5-carboxylcytosine promotes CTCF binding to suboptimal sequences genome-wide
Authors: Nanan Kyster K. , Sturgill David M. , Prigge Maria F. , Thenoz Morgan , Dillman Allissa A. , Mandler Mariana D. , Oberdoerffer Shalini 
Published: November 2018

SummaryThe mechanisms supporting dynamic regulation of CTCF binding sites remain poorly understood. Here we describe the TET-catalyzed 5-methylcytosine derivative, 5-carboxylcytosine (5caC) as a factor driving new CTCF binding within genomic DNA. Through a combination of in vivo and in vitro approaches, we reveal that 5caC generally strengthens CTCF association with DNA and facilitates binding to suboptimal sequences. Dramatically, profiling of CTCF binding in a cellular model that accumulates genomic 5caC identified ∼13,000 new CTCF sites. The new sites were enriched for overlapping 5caC and were marked by an overall reduction in CTCF motif strength. As CTCF has multiple roles in gene expression, these findings have wide-reaching implications and point to induced 5caC as a potential mechanism to achieve differential CTCF binding in cells.
Human Navigation Without and With Vision - the Role of Visual Experience and Visual Regions
Authors: Maidenbaum Shachar , Chebat Daniel-Robert , Amedi Amir 
Published: November 2018

AbstractHuman navigation relies on a wide range of visual retinotopic cortical regions yet the precise role that these regions play in navigation remains unclear. Are these regions mainly sensory input channels or also modality-independent spatial processing centers? Accordingly, will they be recruited for navigation also without vision, such as via audition? Will visual experience, or the lack thereof, affect this recruitment? Sighted, congenitally blind and sighted-blindfolded participants actively navigated virtual mazes during fMRI scanning before and after navigating them in the real world. Participants used the EyeCane visual-to-auditory navigation aid for non-visual navigation.We found that retinotopic regions, including both dorsal stream regions (e.g. V6) and primary regions (e.g. peripheral V1), were selectively recruited for non-visual navigation only after the participants mastered the EyeCane demonstrating rapid plasticity for non-visual navigation. The hippocampus, considered the navigation network’s core, displayed negative BOLD in all groups.Our results demonstrate the robustness of the retinotopic nodes modality-independent spatial role in non-visual human navigation to lifelong visual-deprivation, demonstrating that visual input during development is not required for their recruitment. Furthermore, our results with the blindfolded group demonstrate this recruitment’s robustness even to brief blindfolding, but only after brief training, demonstrating rapid task based plasticity. These results generalize the wider framework of task-selectivity rather than input-modality as a brain organization principle to dorsal-stream retinotopic areas and even for the first time to the primary visual cortex.HighlightsBoth visual and non-visual navigation recruit retinotopic regionsAfter training blindfolded subjects selectively recruit V1 & V6 for navigationThis holds also for participants with no visual experience (congenitally blind)The medial temporal lobe showed non-selective Negative BOLD in all groupsDeclaration of interestsAll authors declare that they have no conflicts of interests.
A multi-site MicroLED Optrode Array for Neural Interfacing
Authors: McAlinden Niall , Cheng Yunzhou , Scharf Robert , Xie Enyuan , Gu Erdan , Dawson Martin D. , Reiche Christopher , Sharma Rohit , Tathireddy Prashant , Rieth Loren , Blair Steve , Mathieson Keith 
Published: November 2018

AbstractWe present an electrically addressable optrode array capable of delivering light to 181 sites in the brain, each providing sufficient light to optogenetically excite hundreds of neurons in vivo, developed with the aim to allow behavioural studies in large mammals. The device is a glass microneedle array directly integrated with a custom fabricated microLED device, which delivers light to 100 needle tips and 81 interstitial surface sites, giving 2-level optogenetic excitation of neurons in vivo. Light delivery and thermal properties are evaluated, with the device capable of peak irradiances > 80 mW/mm2 per needle at 50 ms pulse widths with tissue temperature increase less than 1 °C. Future designs are explored through optical and thermal modelling and benchmarked against the current device.
Flexible, fast and selective genetic manipulation of the vertebrate CNS with misPiggy
Authors: Slezak Michal , Vin Filip de , Shinmyo Yohei , Batiuk Mykhailo Y. , Rincon Melvin Y. , Pando Carmen Menacho , Urschitz Johann , Moisyadi Stefan , Schnütgen Frank , Kawasaki Hiroshi , Holt Matthew G. 
Published: November 2018

AbstractIn utero electroporation is increasingly used for manipulation of gene expression in the mammalian central nervous system. Here we created a transposon-based single plasmid system, that allows long-term conditional loss- and gain-of-function experiments across major CNS cell types. The system is widely applicable across species, allowing fast, flexible and cheap cell manipulation in previously non-transgenic species.
Non-uniform genetic effect sizes of variants associated with refractive error suggests gene-gene or gene-environment interactions are pervasive
Authors: Pozarickij Alfred , Williams Cathy , Hysi Pirro , Guggenheim Jeremy A. ,   the UK Biobank Eye and Vision Consortium
Published: November 2018

AbstractRefractive error is a complex ocular trait controlled by genetic and environmental factors. Genome-wide association studies (GWAS) have identified approximately 150 genetic variants associated with refractive error. Among the known environmental factors, education, near-work and time spent outdoors have been demonstrated to have the strongest associations. Currently, the extent of gene-environment or gene-gene interactions in myopia is unknown. Here we show that the majority of genetic variants associated with refractive error show evidence of effect size heterogeneity, which is a hallmark feature of genetic interactions. Using conditional quantile regression, we observed that 88% of genetic variants associated with refractive error have at least nominally-significant non-uniform, non-linear profiles across the refractive error distribution. SNP effects tend to be strongest at the phenotype extremes and have weaker effects in emmetropes. A parsimonious explanation for these findings is that gene-environment or gene-gene interactions in refractive error are pervasive.Author summaryThe prevalence of myopia (nearsightedness) in the United States and East Asia has almost doubled in the past 30 years. Such a rapid rise in prevalence cannot be explained by genetics, which implies that environmental (lifestyle) risk factors play a major role. Nevertheless, diverse approaches have suggested that genetics is also important, and indeed approximately 150 distinct genetic risk loci for myopia have been discovered to date. One attractive explanation for the evidence implicating both genes and environment in myopia is gene-environment (GxE) interaction (a difference in genetic effect in individuals exposed to a high vs. low level of an environmental risk factor). Past studies aiming to discover GxE interactions in myopia have met with limited success, perhaps because information on lifestyle exposures during childhood has rarely been available. Here we used an agnostic approach that does not require information about specific lifestyle exposures in order to detect ‘signatures’ of GxE interaction. We found compelling evidence for widespread genetic interactions in myopia, with 88% of 150 known myopia genetic susceptibility loci showing an interaction signature. These findings suggest that GxE interactions in myopia are pervasive.
The shaping of sustainability assurance through the competition between accounting and non-accounting providers
Authors: Farooq Muhammad Bilal , de Villiers Charl 
Published: November 2018

PurposeThe purpose of this paper is to examine the competition between accounting sustainability assurance providers (ASAPs) and non-accounting sustainability assurance providers (NASAPs), and how this competition influences the institutionalization of the evolving field of sustainability assurance.Design/methodology/approachAn interpretivist research methodology, guided by an institutional work perspective, is used to analyze interviews with 15 SAPs and 35 sustainability reporting managers (SRMs) in Australia and New Zealand.FindingsASAPs prefer to use International Standard on Assurance Engagements 3000 (ISAE3000), because it is well recognized in the profession, adheres to ASAPs’ regulatory requirements, and mirrors their financial audit methodologies. This preference influences ASAPs’ institutional work as they compete against NASAPs and how they institutionalize sustainability assurance. ASAPs’ institutional works include presenting sustainability assurance as similar to a financial audit, arguing in support of a single provider for financial audits and sustainability assurance, and undermining NASAPs and their preferred sustainability assurance standard, AA1000 Assurance Standard (AA1000AS), by appealing to senior management. In comparison, NASAPs promote AA1000AS as a specialist standard among SRMs, emphasizing the standard’s sustainability enhancing qualities and its flexibility, while discrediting ASAPs and ISAE3000 as out of touch with sustainability objectives.Research limitations/implicationsA new conceptual model is constructed that can be used in institutional work research.Practical implicationsThe accounting profession is encouraged to consider more flexible, innovative methods in new assurance markets. This involves using new assurance standards as well as developing specialist standards for new forms of assurance. Regulation over sustainability assurance could be helpful, but regulators should be careful not to stifle competition in this evolving field.Originality/valueThis paper examines how competition between ASAPs and NASAPs influences the institutionalization of sustainability assurance. The paper offers a new model for the analysis of institutional work, which could be used by researchers, new insights into the emerging field of sustainability assurance, as well as a figure and discussion that clarifies the broader implications of the findings.
Role of phytic acid in the corrosion protection of epoxy-coated rusty steel
Authors: Zhang Yingjun , Cui Xue-Jun , Shao Yawei , Wang Yanqiu , Meng Guozhe , Lin Xiu-Zhou , Zhong Dongquan , Wang Dajian 
Published: November 2018

PurposeThis paper aims to prepare a residual rust epoxy coating by adding different quantities of phytic acid (PA) on the surface of the rusty steel and investigate the corrosion protection of PA and its action mechanisms.Design/methodology/approachA residual rust epoxy coating by adding different quantities of PA was prepared on the surface of the rusty steel. The influence of PA on the corrosion resistance of epoxy-coated rusty steel was investigated by means of electrochemical impedance spectroscopy and adhesion testing.FindingsResults indicated that PA can substantially improve the corrosion resistance of epoxy-coated rusty steel. This improvement is due to the reaction of PA with residual rust and generation of new compounds with protection properties and increased adhesive strength effects on the coating/metal interface. The coating showed better protection performance when 2 per cent PA was added.Originality/valueConsidering the structure of the active groups, PA has strong chelating capability with many metal ions and can form stable complex compounds on the surface of a metal substrate, thereby improving corrosion resistance. In recent years, PA has been reported to be useful in the conversion of coatings or as green corrosion inhibitor. To the best of the authors’ knowledge, few studies have reported the use of PA as a rust converter or residual rust coating. The present work aims to improve the corrosion resistance of residual rust epoxy coating by adding PA.
Exploratory analysis of Publons metrics and their relationship with bibliometric and altmetric impact
Authors: Ortega Jose Luis 
Published: November 2018

Purpose
The purpose of this paper is to analyse the metrics provided by Publons about the scoring of publications and their relationship with impact measurements (bibliometric and altmetric indicators).

Design/methodology/approach
In January 2018, 45,819 research articles were extracted from Publons, including all their metrics (scores, number of pre and post reviews, reviewers, etc.). Using the DOI identifier, other metrics from altmetric providers were gathered to compare the scores of those publications in Publons with their bibliometric and altmetric impact in PlumX, Altmetric.com and Crossref Event Data.

Findings
The results show that: there are important biases in the coverage of Publons according to disciplines and publishers; metrics from Publons present several problems as research evaluation indicators; and correlations between bibliometric and altmetric counts and the Publons metrics are very weak (r<0.2) and not significant.

Originality/value
This is the first study about the Publons metrics at article level and their relationship with other quantitative measures such as bibliometric and altmetric indicators.
A comparative study on economic production quantity (EPQ) model under space constraint with different kinds of data
Authors: Karbassi Yazdi Amir , Kaviani Mohamad Amin , Sarfaraz Amir Homayoun , Cárdenas-Barrón Leopoldo Eduardo , Wee Hui-Ming , Tiwari Sunil 
Published: November 2018

PurposeThe purpose of this paper is to develop a multi-item economic production quantity (EPQ) strategy under grey environment and space constraint. Since the “demand” cannot be predicted with certainty, it is assumed that data behave under grey environment and compare the proposed inventory model with other studies using crisp or fuzzy environments.Design/methodology/approachThis paper is to optimise the cycle time and total cost of the multi-item EPQ inventory model. For this purpose, the Lagrangian coefficient is used to solve the constrained optimisation problem. The grey relational analysis approach and grey data are applied in developing the EPQ inventory model.FindingsThe results are compared with the analysis using crisp and fuzzy data. Sensitivity analysis is done to illustrate the effect of parameter variations on the optimal solution. The results of the study demonstrate that crisp data outperform the other two data in all scales problems in terms of cycle time and cost; grey data perform better in all scales problems than fuzzy data.Originality/valueThe contribution of this research is the use of grey data in developing the EPQ inventory model with space constraint.
An energy efficient load balanced cluster-based routing using ant colony optimization for WSN
Authors: Guleria Kalpna , Verma Anil Kumar 
Published: November 2018

Purpose
Wireless sensor networks (WSNs) have emerged as one of the most promising technology in our day-to-day life. Limited network lifetime and higher energy consumption are two most critical issues in WSNs. The purpose of this paper is to propose an energy-efficient load balanced cluster-based routing protocol using ant colony optimization (LB-CR-ACO) which ultimately results in enhancement of the network lifetime of WSNs.

Design/methodology/approach
The proposed protocol performs optimal clustering based on cluster head selection weighing function which leads to novel cluster head selection. The cluster formation uses various parameters which are remaining energy of the nodes, received signal strength indicator (RSSI), node density and number of load-balanced node connections. Priority weights are also assigned among these metrics. The cluster head with the highest probability will be selected as an optimal cluster head for a particular round. LB-CR-ACO also performs a dynamic selection of optimal cluster head periodically which conserves energy, thereby using network resources in an efficient and balanced manner. ACO is used in steady state phase for multi-hop data transfer.

Findings
It has been observed through simulation that LB-CR-ACO protocol exhibits better performance for network lifetime in sparse, medium and dense WSN deployments than its peer protocols.

Originality/value
The proposed paper provides a unique energy-efficient LB-CR-ACO for WSNs. LB-CR-ACO performs novel cluster head selection using optimal clustering and multi-hop routing which utilizes ACO. The proposed work results in achieving higher network lifetime than its peer protocols.