bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measurable residual disease, conditioning regimen intensity, and age predict outcome of allogeneic hematopoietic cell transplantation for acute myeloid leukemia in first remission: A registry analysis of 2292 patients by the Acute Leukemia Working Party European Society of Blood and Marrow Transplantation
Authors: Gilleece Maria H. , Labopin Myriam , Yakoub‐Agha Ibrahim , Volin Liisa , Socié Gerard , Ljungman Per , Huynh Anne , Deconinck Eric , Wu Depei , Bourhis Jean Henri , Cahn Jean Yves , Polge Emmanuelle , Mohty Mohamad , Savani Bipin N. , Nagler Arnon 
Published: July 2018

AbstractPatients with acute myeloid leukemia (AML) in morphological first complete remission (CR1) pre‐allogeneic hematopoietic cell transplantation (HCT) may have measurable residual disease (MRD) by molecular and immunophenotyping criteria. We assessed interactions of MRD status with HCT conditioning regimen intensity in patients aged <50 years (y) or ≥50y. This was a retrospective study by the European Society for Blood and Marrow Transplantation registry. Patients were >18y with AML CR1 MRD NEG/POS and recipients of HCT in 2000‐2015. Conditioning regimens were myeloablative (MAC), reduced intensity (RIC) or non‐myeloablative (NMA). Outcomes included leukemia free survival (LFS), overall survival (OS), relapse incidence (RI), non‐relapse mortality (NRM), chronic graft‐vs‐host (cGVHD), and GVHD‐free and relapse‐free survival (GRFS). The 2292 eligible patients were categorized into four paired groups: <50y MRD POS MAC (N = 240) vs RIC/NMA (N = 58); <50y MRD NEG MAC (N = 665) vs RIC/NMA (N = 195); ≥50y MRD POS MAC (N = 126) vs RIC/NMA (N = 230), and ≥50y MRD NEG MAC (N = 223) vs RIC/NMA (N = 555). In multivariate analysis RIC/NMA was only inferior to MAC for patients in the <50y MRD POS group, with worse RI (HR 1.71) and LFS (HR 1.554). Patients <50Y MRD NEG had less cGVHD after RIC/NMA HCT (HR 0.714). GRFS was not significantly affected by conditioning intensity in any group. Patients aged <50y with AML CR1 MRD POS status should preferentially be offered MAC allo‐HCT. Prospective studies are needed to address whether patients with AML CR1 MRD NEG may be spared the toxicity of MAC regimens. New approaches are needed for ≥50y AML CR1 MRD POS.
The Structure of the Cobalt Oxide/Au Catalyst Interface in Electrochemical Water Splitting
Authors: Fester Jakob , Makoveev Anton , Grumelli Doris , Gutzler Rico , Sun Zhaozong , Rodríguez‐Fernández Jonathan , Kern Klaus , Lauritsen Jeppe V. 
Published: July 2018

AbstractThe catalytic synergy between cobalt oxide and gold leads to strong promotion of the oxygen evolution reaction (OER)—one half‐reaction of electrochemical water splitting. However, the mechanism behind the enhancement effect is still not understood, in part due to a missing structural model of the active interface. Using a novel interplay of cyclic voltammetry (CV) for electrochemistry integrated with scanning tunneling microscopy (STM) and X‐ray photoelectron spectroscopy (XPS) on an atomically defined cobalt oxide/Au(111) system, we reveal here that the supporting gold substrate uniquely favors a flexible cobalt‐oxyhydroxide/Au interface in the electrochemically active potential window and thus suppresses the formation of less active bulk cobalt oxide morphologies. The findings substantiate why optimum catalytic synergy is obtained for oxide coverages on gold close to or below one monolayer, and provide the first morphological description of the active phase during electrocatalysis.
Der Mackay‐Cluster [Cu43Al12](Cp*)12: Ein offenschaliges 67‐Elektronen‐Superatom mit metallähnlicher elektronischer Struktur
Authors: Weßing Jana , Ganesamoorthy Chelladurai , Kahlal Samia , Marchal Rémi , Gemel Christian , Cador Olivier , Da Silva Augusto C. H. , Da Silva Juarez L. F. , Saillard Jean‐Yves , Fischer Roland A. 
Published: July 2018

AbstractDer paramagnetische Cluster [Cu43Al12](Cp*)12 entsteht bei der Reaktion von [CuMes]5 mit [AlCp*]4 (Cp*=η5‐C5Me5; Mes=Mesityl). Dieser ausschließlich durch Kohlenwasserstoff‐Liganden stabilisierte “magische” Cluster mit 55 Atomen im Clusterkern weist eine ummantelte Ikosaeder‐Struktur des Mackay‐Typs auf. Die Superatomkonfiguration von 67 Elektronen und offener Valenzschale ist einzigartig, wobei drei ungepaarte Elektronen schwach antibindende Jellium‐Zustände besetzen. Diese Situation deutet auf die Bildung eines Leitungsbands hin, was durch die Temperaturunabhängigkeit des Magnetismus bestätigt wird. Die sterische Abschirmung durch 12 Cp*‐Liganden unterdrückt die intrinsische polyradikalische Reaktivität des Cu43Al12‐Kerns.
Techniques for chromosomal integration and expression optimization in Escherichia coli
Authors: Ou Bingming , Garcia Carolina , Wang Yejun , Zhang Weiping , Zhu Guoqiang 
Published: July 2018

AbstractDue to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins.
Dynamic microRNA activity identifies therapeutic targets in trastuzumab‐resistant HER2+breast cancer
Authors: Decker Joseph T. , Hall Matthew S. , Blaisdell Rachel B. , Schwark Kallen , Jeruss Jacqueline S. , Shea Lonnie D. 
Published: July 2018

AbstractMicroRNAs (miRNAs) are implicated in numerous physiologic and pathologic processes, such as the development of resistance to chemotherapy. Determining the role of miRNAs in these processes is often accomplished through measuring miRNA abundance by polymerase chain reaction, sequencing, or microarrays. We have developed a system for the large‐scale monitoring of dynamic miRNA activity and have applied this system to identify the contribution miRNA activity to the development of trastuzumab resistance in a cell model of HER2+breast cancer. MiRNA activity measurements identified significantly different activity levels between BT474 cells (HER2+breast cancer) and BT474R cells (HER2+breast cancer cells selected for resistance to trastuzumab). We created a library of 32 miRNA reporter constructs, which were delivered by lentiviral transduction into cells, and miRNA activity was quantified by bioluminescence imaging. Upon treatment with the bioimmune therapy, trastuzumab, the activity of 11 miRNAs were significantly altered in parental BT474 cells, and 20 miRNAs had significantly altered activity in the therapy‐resistant BT474R cell line. A combination of statistical, network and classification analysis was applied to the dynamic data, which identified miR‐21 as a controlling factor in trastuzumab response. Our data suggested downregulation of miR‐21 activity was associated with resistance, which was confirmed in an additional HER2+breast cancer cell line, SKBR3. Collectively, the dynamic miRNA activity measurements and analysis provided a system to identify new potential therapeutic targets in treatment‐resistant cancers.
Dearomatization of the PCP Pincer Ligand in a ReV Oxo Complex
Authors: Kosanovich Alex J. , Komatsu Christopher H. , Bhuvanesh Nattamai , Pérez Lisa M. , Ozerov Oleg V. 
Published: July 2018

AbstractA high‐valent, rhenium(V) oxo complex (PCP)ReOCl2 (1; PCP=bis(2,6‐di‐tert‐butylphosphinomethyl)phenyl) undergoes a deprotonation and “dearomatization” upon treatment with LiN(SiMe3)2 to give (P*CP)ReOCl (2 a), in which Re is bound to a new dianionic P*CP ligand. Compound 2 a was studied spectroscopically, structurally, and computationally and was determined to have non‐negligible Re=C multiple bond character, leading to its formulation as a new pseudo‐carbenoid species. Reaction of 2 a or its iodo analogue (P*CP)ReOI (2 b) with CO2 provided access to (PCP)ReOX(CO2) (X=Cl or I, 3 a/b), the product of 1,3‐cycloaddition and C−C bond formation.
Two‐Step Synthesis of Unsymmetrical Diaryl Sulfides by Electrophilic Thiolation of Non‐functionalized (Hetero)arenes
Authors: Böhm Marvin J. , Golz Christopher , Rüter Isabelle , Alcarazo Manuel 
Published: July 2018

AbstractThis article reports the efficient preparation of a series of unsymmetrically substituted thioethers through a two‐step procedure consisting of an initial metal‐free C−H sulfenylation of electron‐rich (hetero)arenes with newly prepared succinylthioimidazolium salts. Subsequent reaction of the arylthioimidazolium intermediates with Grignard reagents afford the desired thioethers. The synthetic protocol described is modular, scalable, and high yielding, and provides access to sulfides that are not easy to obtain through the existing methodologies. Importantly, no prefunctionalization of the initial (hetero)arene is required.
Cholesterol: A Key in the Pathogenesis of Alzheimer's Disease
Authors: Banerjee Samya , Mukherjee Sanjoy 
Published: July 2018

AbstractHerein we highlight recent advances in our understanding of the role of cholesterol in Alzheimer′s disease (AD). It has been proposed that cholesterol could enhance the risk of AD, and the interaction between cholesterol and amyloid‐β peptide 42 (Aβ42) has been studied extensively, yet until recently, the specific interaction mechanisms between them and how this affects Aβ42 aggregation had not yet been fully explored and had remained ambiguous. Vendruscolo and co‐workers addressed these issues in their recent article entitled “Cholesterol catalyses Aβ42 aggregation through a heterogeneous nucleation pathway in the presence of lipid membranes” (Habchi et al., Nat. Chem. 2018, 10, 673). In this article, the authors revealed the mechanism behind cholesterol‐catalyzed Aβ42 aggregation, providing the potential to address the molecular origins of AD, thereby opening a new avenue for effective AD therapy.
CoO/Co‐Activated Porous Carbon Cloth Cathode for High Performance Li–S Batteries
Authors: Ren Wenchen , Ma Wei , Umair Malik Muhammad , Zhang Shufen , Tang Bingtao 
Published: July 2018

AbstractLi–S batteries are one of the most promising candidates for the next generation of batteries because of their high theoretical specific capacity of 1675 mA h g−1. However, the dissolution of polysulfide causes severe capacity fading during cycling. Herein, a 3 D CoO/Co‐activated free‐standing porous carbon fiber cloth (CoO/Co@PCF) is designed to suppress polysulfide diffusion from the cathode. The metallic Co and polar CoO can anchor polysulfide through Co−S ionic bonding and the pores of the carbon cloth can provide abundant active sites for electrochemical reactions. Specifically, sulfur in the cathode is loaded by sulfur vapor deposition, which is scalable for fabrication and adjustable to the sulfur‐loading amount. The CoO/Co@PCF cathode with sulfur loading of 3 mg cm−2 displays high discharge capacities of 1214.2 and 684.3 mA h g−1 at 0.1 and 2 C, respectively. When the sulfur loading is increased to 5.4 mg cm−2, low capacity fading of 0.075 % per cycle over 100 cycles at 0.5 C is still observed.
Facilely Synthesized spiro[fluorene‐9,9′‐phenanthren‐10′‐one] in Donor–Acceptor–Donor Hole‐Transporting Materials for Perovskite Solar Cells
Authors: Chen Yih‐Chun , Huang Shao‐Ku , Li Shao‐Sian , Tsai Yao‐Yu , Chen Chih‐Ping , Chen Chun‐Wei , Chang Yuan Jay 
Published: July 2018

AbstractWe have demonstrated two novel donor–acceptor–donor (D–A–D) hole‐transport material (HTM) with spiro[fluorene‐9,9′‐phenanthren‐10′‐one] as the core structure, which can be synthesized through a low‐cost process in high yield. Compared to the incorporation of the conventional HTM of commonly used 2,2′,7,7′‐tetrakis[N,N‐di(4‐methoxyphenyl)amino]‐9,9′‐spirobifluorene (Spiro‐OMeTAD), the synthesis process is greatly simplified for the presented D–A–D materials, including a minimum number of purification processes. This results in an increased production yield (>55 %) and suppressed production cost (<30 $ g−1), in addition to high power conversion efficiency (PCE) in perovskite solar cells (PSCs). The PCE of a PSC using our D–A–D HTM reaches 16.06 %, similar to that of Spiro‐OMeTAD (16.08 %), which is attributed to comparable hole mobility and charge‐transfer efficiency. D–A–D HTMs also provide better moisture resistivity to prolong the lifetime of PSCs under ambient conditions relative to their Spiro‐OMeTAD counterparts. The proposed new type of D–A–D HTM has shown promising performance as an alternative HTM for PSCs and can be synthesized with high production throughput.
Disordered Bilayered V2O5 ⋅ nH2O Shells Deposited on Vertically Aligned Carbon Nanofiber Arrays as Stable High‐Capacity Sodium Ion Battery Cathodes ⋅
Authors: Brown Emery , Acharya Jagaran , Elangovan Ayyappan , Pandey Gaind P. , Wu Judy , Li Jun 
Published: July 2018

AbstractDue to the large radius of Na+ ions, it has been challenging to find suitable host materials for sustainable sodium ion batteries (SIBs). This study investigates sputter‐coated thin V2O5 shells on vertically aligned carbon nanofiber (VACNF) arrays as a novel three‐dimensional (3D) core‐shell material for SIB cathodes. SEM, TEM, XRD and Raman spectroscopy revealed that the as‐deposited V2O5 shell has a highly disordered bilayered V2O5 ⋅ nH2O structure with a large interlayer spacing of 11.0 Å, which can accommodate Na+ ions better than orthorhombic α‐V2O5 crystals. This hydrated metastable structure has been systematically characterized for Na+ storage. A high initial insertion capacity of 277 mAh g−1 can be achieved at a current density of 250 mA g−1 in the potential window of 4.0–1.0 V (vs Na/Na+). Using higher charge‐discharge rate or narrower potential windows, the electrode becomes more reversible, able to reach a coulombic efficiency of ∼99 %. Cyclic voltammetry, galvanostatic charge‐discharge and electrochemical impedance spectroscopy measurements indicate that the Na+ storage is dominated by a large pseudocapacitive contribution due to fast surface reactions, which facilitates the improved stability and high power density. Such highly disordered bilayered V2O5 ⋅ nH2O material in the 3D core‐shell architecture provides new insights for developing future SIB materials.
Age‐associated changes in waking hippocampal sharp‐wave ripples
Authors: Cowen Stephen L. , Gray Daniel T. , Wiegand Jean‐Paul L. , Schimanski Lesley A. , Barnes Carol A. 
Published: July 2018

AbstractHippocampal sharp‐wave ripples are brief high‐frequency (120–250 Hz) oscillatory events that support mnemonic processes during sleep and awake behavior. Although ripples occurring during sleep are believed to facilitate memory consolidation, waking ripples may also be involved in planning and memory retrieval. Recent work from our group determined that normal aging results in a significant reduction in the peak oscillatory frequency and rate‐of‐occurrence of ripples during sleep that may contribute to age‐associated memory decline. It is unknown, however, how aging alters waking ripples. We investigated whether characteristics of waking ripples undergo age‐dependent changes. Sharp‐wave ripple events were recorded from the CA1 region of the hippocampus in old (n = 5) and young (n = 6) F344 male rats as they performed a place‐dependent eyeblink conditioning task. Several novel observations emerged from this analysis. First, although aged rats expressed more waking ripples than young rats during track running and reward consumption, this effect was eliminated, and, in the case of track‐running, reversed when time spent in each location was accounted for. Thus, aged rats emit more ripples, but young rats express a higher ripple rate. This likely results from reduced locomotor activity in aged animals. Furthermore, although ripple rates increased as young rats approached rewards, rates did not increase in aged rats, and rates in aged and young animals were not affected by eyeblink conditioning. Finally, although the oscillatory frequency of ripples was lower in aged animals during rest, frequencies in aged rats increased during behavior to levels indistinguishable from young rats. Given the involvement of waking ripples in memory retrieval, a possible consequence of slower movement speeds of aged animals is to provide more opportunity to replay task‐relevant information and compensate for age‐related declines in ripple rate during task performance.
Modification of α2,6‐sialylation mediates the invasiveness and tumorigenicity of non‐small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway
Authors: Yuan Qingmin , Chen Xixi , Han Yang , Lei Ting , Wu Qiang , Yu Xiao , Wang Liping , Fan Zhe , Wang Shujing 
Published: July 2018

AbstractThe alterations of sialylation on cell surface N‐glycans due to overexpression of different sialyltransferases play a vital role in tumorigenesis and tumor progression. The β‐galactoside α2–6‐sialyltransferase 1 (ST6Gal‐I) has been reported to be highly expressed in several cancers, including breast cancer, hepatocellular cancer and colon carcinoma. However, the roles and underlying mechanisms of ST6Gal‐I in non‐small cell lung cancer (NSCLC) still need to be elucidated. In this study, we determined that mRNA levels of ST3GAL1, ST6GALNAC3 and ST8SIA6 were remarkably reduced in lung cancer tissues and cells, whereas ST6GAL1 level significantly increased. The mRNA, protein and glycan levels of ST6Gal‐I were higher in lung cancer tissues and cells. Moreover, down‐regulation of ST6Gal‐I decreased protein levels of Jagged1, DLL‐1, Notch1, Hes1, Hey1, matrix‐metalloproteinases (MMPs) and VEGF, and suppressed proliferation, migration and invasion capabilities of A549 and H1299 cells in vitro. In vivo, ST6Gal‐I silencing suppressed tumorigenicity of NSCLC cells in athymic nude mice via the Notch1/Hes1/MMPs pathway. In addition, overexpression of Notch1 rescued the reduced growth and metastasis of A549 and H1299 cells resulted by ST6Gal‐I silencing. Modification of α2,6‐sialylation positively associates with lung cancer progression, thereby indicating that ST6Gal‐I may mediate the invasiveness and tumorigenicity of NSCLC cells via the Notch1/Hes1/MMPs pathway both in vitro and in vivo. Thus, our results provide a novel therapeutic approach for blocking metastasis in lung cancer patients.
Seroprevalence of hepatitis C virus among the newcomer students, Kafrelsheikh University, Egypt
Authors: El Batae Hassan , Amer Ibrahim , Kobtan Abdelrahman , Saied Shimaa M. , Ghazy Amany , Elkalla Ferial , El Sharawy Shimaa 
Published: July 2018

BackgroundHepatitis C virus (HCV) constitutes a global public health problem in Egypt, as it has the highest worldwide prevalence. This study aimed at determining the seroprevalence of HCV among the newcomer students of Kafrelsheikh University, Egypt.MethodsA cross‐sectional serosurvey was conducted including 9049 students. Medical examination, ultrasonography, and laboratory investigations were done. Liver function tests and HCV antibody testing were carried out for all students who gave an informed consent; HCV‐RNA polymerase chain reaction was performed for students with positive HCV antibody testing.ResultsThe mean age of screened students were 18.6 ± 0.39 years. In total, 4233 (46.8%) were males and 4816 (53.2%) were females. Using HCV antibody testing, only 25 students (0.0028%) had positive antibodies; among them, 24 students (0.0026%) had HCV RNA positive; the study showed none statistically significant higher percentage of HCV infection among males (13 out of 24, 54.2%) than females (11 out of 24, 48.5%), P > 0.05. The results of liver function tests were not significantly different between the HCV‐positive and HCV‐negative students. However, the liver transaminase enzymes were significantly higher (
P < 0.0001) in HCV positive students compared to the negative ones, despite its mean values did not exceed the upper normal level. HCV infection among young Egyptian generations showed a marked decline.ConclusionPrevalence of HCV infection among young Egyptian generations had markedly decline, indicating the start of successful control of HCV infection.
Prevalence of C‐C chemokine receptor type 5 tropism among human immunodeficiency virus 1–infected patients in South Korea
Authors: Song Je Eun , Ahn Mi Young , Kim Woo Joo , Kim Shin‐Woo , Lee Jin Soo , Ku Nam Su , Kim Joon Hyung , Kim Ki‐Hyon , Ann Heawon , Choi Jun Yong 
Published: July 2018

ObjectiveThe discovery of two main coreceptors for human immunodeficiency virus (HIV), C‐C chemokine receptor type 5 (CCR5), and C‐X‐C chemokine receptor type 4 has led to a better understanding of the interaction between HIV envelope and host cells, and development of new therapeutic approaches. The purpose of this study was to estimate the prevalence of CCR5 tropism among HIV‐1–infected Koreans and identify the predictors for CCR5 tropism.MethodsWe enrolled 250 HIV‐1–infected subjects from four medical centers of three different cities in South Korea between April 2013 and May 2014. Genotypic assay for identifying coreceptor tropism of HIV‐1 was performed with HIV RNA or HIV DNA. Nested polymerase chain reaction and population‐based sequencing for the V3 region (HXB2 position 6225‐7758) of the envelope were performed with HIV RNA or proviral DNA. Proviral DNA was used if the viral load of the subject was below 2000 copies/mL. Genotypic tropism was determined by a web‐based bioinformatics tool (http://coreceptor.bioinf.mpi‐inf.mpg.de/).ResultsAmong 250 individuals enrolled, only 143 subjects could be analyzed for genotypic tropism assay with HIV RNA or proviral DNA. The prevalence of CCR5 tropism was 69.2% (N = 99). We could not identify any significant clinical or epidemiological predictors for CCR5 tropism among enrolled subjects.ConclusionsThe prevalence of CCR5 tropism in HIV‐1–infected Korean individuals was 69.2%. Since we cannot predict coreceptor tropism by clinical factors, tropism assay should be performed before treatment with the CCR5 antagonist.
Impact of expiratory strength training in amyotrophic lateral sclerosis: Results of a randomized, sham‐controlled trial
Authors: Plowman Emily K. , Tabor‐Gray Lauren , Rosado K. Michelle , Vasilopoulos Terrie , Robison Raele , Chapin Jennifer L. , Gaziano Joy , Vu Tuan , Gooch Clifton 
Published: July 2018

ABSTRACTIntroductionThe purpose of this study was to determine the impact of an in‐home expiratory muscle strength training (EMST) program on pulmonary, swallow, and cough function in individuals with amyotrophic lateral sclerosis (ALS).MethodsEMST was tested in a prospective, single‐center, double‐blind, randomized, controlled trial of 48 ALS individuals who completed 8 weeks of either active EMST (n = 24) or sham EMST (n = 24). The primary outcome to assess treatment efficacy was change in maximum expiratory pressure (MEP). Secondary outcomes included: cough spirometry; swallowing; forced vital capacity; and scoring on the ALS Functional Rating Scale—Revised.ResultsTreatment was well tolerated with 96% of patients completing the protocol. Significant differences in group change scores were noted for MEP and Dynamic Imaging Grade of Swallowing Toxicity scores (P < 0.02). No differences were noted for other secondary measures.DiscussionThis respiratory training program was well‐tolerated and led to improvements in respiratory and bulbar function in ALS. Muscle Nerve 59:40–46, 2019