bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penerapan Model Children Learning in Science Untuk Meningkatkan Hasil Belajar Siswa Kelas VIII C SMP Muhammadiyah 1 Banjarmasin
Authors: Septiyani Trias , Hartini Sri , Salam Abdul 
Published: August 2019

Penelitian ini bertujuan untuk meningkatkan hasil belajar siswa kelas VIII SMP Muhammadiyah 1 Banjarmasin dengan menerapkan model pembelajaran Children Learning In Science (CLIS). Jenis penelitian ini adalah PTK terdiri dari 2 siklus. Subjek penelitian merupakan seluruh siswa kelas VIII C SMP Muhammadiyah 1 Banjarmasin yang berjumlah 20 orang. Instrumen penelitian ini adalah lembar pengamatan keterlaksanaan RPP, lembar pengamatan aktivitas siswa, dan tes hasil belajar. Temuan membuktikan : (1) keterlaksanaan RPP siklus I sebesar 67,16% yang berkategori baik dan siklus II sebesar 70,56% yang berkategori baik,(2) aktivitas siswa pada siklus I berkategori cukup aktif dan pada siklus II mengalami peningkatan menjadi berkategori aktif, (3) hasil belajar siswa siklus I sebesar 55,00% (tidak tuntas) dan pada siklus II sebesar 75,00% (tuntas). Berdasarkan hasil temuan, model CLIS mampu meningkatkan hasil belajar serta aktivitas siswa.
Meningkatkan Keterampilan Proses Sains dan Hasil Belajar IPA Melalui Model Pembelajaran Inkuiri Terbimbing
Authors: Rafiah Rafiah , Arifuddin Muhammad , Mahardika Andi Ichsan 
Published: August 2019

Proses belajar mengajar hanya berpusat pada guru, akibatya siswa tidak terlatih untuk mengembangkan keterampilan proses sains dan berimbas pada rendahnya hasil belajar siswa. Penelitian ini bertujuan untk mendeskripsikan peningkatkan hasil belajar dan keterampilan proses sains siswa melalui model pembelajaran inkuiri terbimbing pada mata pelajaran IPA di kelas VIII A SMP Muhammadiyah 1 Banjarmasin. Tujuan khususnya adalah mendeskripsikan: 1) keterlaksanaan Rencana Pelaksanaan Pembelajaran, 2) keterampilan proses sains siswa, dan 3) hasil belajar siswa. Jenis penelitian ini adalah model penelitian tindakan kelas Kemmis dan Mc Taggart yang terdiri dari 2 siklus. Subjek penelitian adalah 20 siswa kelas VIII A SMP Muhammadiyah 1 Banjarmasin. Teknik pengumpulan data berupa observasi dan tes. Hasil penelitian menunjukkan bahwa: 1) Keterlaksanaan RPP meningkat setiap siklusnya, yaitu dengan reliabilitas 99,64% dan 99,77%; 2) Peningkatan keterampilan proses sains siswa (merumuskan masalah, merumuskan hipotesis, melakukan percobaan, menganalisis data, menyimpulkan, dan memprediksi) pada siklus I selama proses pembelajaran sebesar 79,5% dan pada akhir siklus senilai 85,6% dengan kategori sangat baik; dan 3) Peningkatan hasil belajar siswa setelah menggunakan model pembelajaran inkuiri terbimbing adalah 40% yang tuntas pada siklus I dan 80% pada siklus II.
Penerapan Model Pembelajaram 5E Untuk Meningkatkan Aktivitas Siswa
Authors: Soraya Siva , Hartini Sri , Miriam Sarah 
Published: August 2019

Penelitian ini dilatar belakangi oleh rendahnya aktivitas dan hasil belajar siswa. Oleh karena itu dilakukan penelitian yang bertujuan untuk meningkatkan aktivitas siswa dengan menggunakan model pembelajaran 5E (Engangement, Exploration, Explanation, Elaboration, Evaluation). Tujuan khusus penelitian mendeskripsikan: 1) keterlaksanaan rencana pelaksanaan pembelajaran (RPP), 2) aktivitas dan 3) hasil belajar. Jenis penelitian ini menggunakan penelitian tindakan kelas model Kemmis dan Mc Taggart yang terdiri dari dua siklus, setiap siklus terdiri dari dua kali pertemuan yang meliputi perencanaan, tindakan & observasi, dan refleksi. Subjek penelitian ini adalah siswa kelas X B Multimedia SMK Negeri 1 Banjarmasin yang berjumlah 26 orang. Data diperoleh melalui observasi dan tes. Hasil penelitian siklus I dan siklus II yaitu: 1) keterlaksanaan RPP pada siklus I dan II bergategori sangat baik 2) aktivitas siswa pada siklus I dan II berkategori cukup aktif, cukup aktif, aktif, dan sangat aktif 3) hasil belajar siswa pada siklus I dan II berkategori tuntas. Diperoleh simpulan bahwa model pembelajaran 5E dapat meningkatkan aktivitas siswa kelas X B Multimedia SMK Negeri 1 Banjarmasin.
Population Genetic Structure and Habitat Connectivity for Jaguar (Panthera onca) Conservation in Belize
Authors: Menchaca Angelica , Rossi Natalia , Froidevaux Jeremy , Dias-freedman Isabela , Rabinowitz Salisa , Foster Rebecca , Harmsen Bart , Amato George 
Published: August 2019

Abstract
Background: Effective connectivity between jaguar ( Panthera onca ) populations across the American continent will ensure the natural gene flow and the long-term survival of the species throughout its range. Jaguar conservation efforts have focused primarily on connecting suitable habitat in a broad-scale. However, accelerated habitat reduction, limited funding, and the complexity of jaguar behaviour have proven challenging to maintain connectivity between populations effectively. Here we used individual-based genetic analysis in synthesis with landscape permeability models to assess levels of current genetic connectivity and identify alternative corridors for jaguar movement between two core areas in central and southern Belize. Results: We use 12 highly polymorphic microsatellite loci to identify 50 distinct individual jaguars, including 41 males, 3 females and 6 undetermined animals, from scat samples collected in The Cockscomb Basin Wildlife Sanctuary and The Central Belize Corridor. Using Bayesian and multivariate models of genetic structure, we identified one single group across two sampling sites with low genetic differentiation between them. We used fine-scale data on biodiversity features as vegetation types to predict the most probable corridors using least-cost path analysis and circuit theory. Conclusions: The results of our study highlight the importance of expanding the boundaries of the Central Belize Corridor to effectively cover areas that would more easily facilitate jaguar movement between locations.
A high-density genetic linkage map for Chinese perch (Siniperca chuatsi) using genotyping-by-sequencing (GBS)
Authors: Guo Wen-jie , He Shan , Liang Xu-fang , Tian Chang-xu , Dou Ya-qi , Lv Li-yuan 
Published: August 2019

Abstract
Background: Chinese perch, Siniperca chuatsi (Basilewsky), is one of the most commercially important cultured fishes in China. In the present study, a high-density genetic linkage map of Chinese perch was constructed by genotyping-by-sequencing (GBS) technique with an F1 mapping panel containing 190 progenies. Result: A total of 2328 SNPs were assigned to 24 linkage groups (LGs), agreeing with the chromosome haploid number in this species (n = 24). The sex-averaged map covered 97.9% of the Chinese perch genome, with the length of 1694.3 cM and a marker density of 0.7 cM/locus. The number of markers per LG ranged from 57 to 222, with a mean of 97. The length of LGs varied from 43.2 cM to 108.2 cM, with a mean size of 70.6 cM. The recombination rate of females was 1.50 : 1, which was higher than that of males. In order to better understand the distribution pattern of segregation distortion between the two sexes of Chinese perch, the skewed markers were retained and used to reconstruct the sex-specific maps. The 16 SDRs were identified on 10 LGs of the female map, while 12 SDRs on 8 LGs of the male map. Among these LGs, 6 LGs matched between the sex-specific maps. Conclusion: This high-density linkage map could provide a solid basis for identifying QTLs associated with economically important traits, and for implementing marker-assisted selection breeding of Chinese perch.
Characterizing clinical carbapenem-resistant Klebsiella pneumoniae phenotypes and genomic information reveals its molecular epidemiology
Authors: Yu Xiaoling , Zhang Wen , Zhao Zhiping , Ye Chengsong , Zhou Shuyan , Wu Shaogui , Han Lifen , Han Zhaofang , YE HANHUI 
Published: August 2019

Abstract
The enhancing incidence of carbapenem-resistant Klebsiella pneumoniae (CRKP)-mediated infections in Mengchao Hepatobiliary Hospital of Fujian Medical University in 2017 promoted this investigation to study gene phenotypes and resistance genes of emergence regarding the CRKP strains. In current study, seven inpatients are enrolled in the hospital with complete treatments. The carbapenem-resistant K. pneumoniae whole genome is sequenced using MiSeq short-read and Oxford Nanopore long-read sequencing technology. Prophages are identified to assess genetic diversity within CRKP genomes. The investigation encompassed eight CRKP strains that collected from the patients enrolled as well as the environment, which illustrate that bla KPC-2 is responsible for phenotypic resistance in six CRKP strains that K . pneumoniae sequence type (ST-11) is inferred. The plasmid with IncR, ColRNAI and pMLST type with IncF[F33:A-:B-] co-exist in all ST-11 with KPC-2-producing CRKP strains. Along with carbapenemases, all K. pneumoniae strains harbor two or three extended spectrum β-lactamase (ESBL)-producing genes. F osA gene is detected amongst all the CRKP strains. The oqxA and oqxB expressions in CRKP strains may lead to carbapenem resistance since antimicrobials are expelled from pathogenic bacteria by efflux pump. The single nucleotide polymorphisms (SNP) markers are indicated and validated among all CRKP strains, providing valuable clues for distinguishing carbapenem-resistant strains from conventional K. pneumoniae .
Genomic-wide analysis and prediction of genes involved in biosynthesis of polysaccharide and bioactive secondary metabolites in high-temperature-tolerance of wild Flammulina filiformis
Authors: Chen Juan , Li Jia-Mei , Tang Yan-Jing , Ma Ke , Li Bing , Zeng Xu , Liu Xiao-Bin , Li Yang , Yang Zhu-Liang , Xu Wei-Nan , Xie Bao-Gui , Guo Shun-Xing 
Published: August 2019

Abstract
Background: Flammulina filiformis (=Asian “F.velutipes”) is a popular commercial edible mushroom. Many bioactive compounds such as polysaccharides and sesquiterpenoids with medicinal effects have been isolated and identified, but their biosynthesis and regulation in molecular level is unclear. In this study, we sequenced the genome of the wild strain F. filiformis Liu 355, predicated the biosynthetic gene clusters (BGCs) and profiled these genes expression between wild and cultivar strains and among different development stages of the wild strain of F. filiformis by a comparative transcriptomic analysis.
Results: The results revealed that the genome of the F. filiformis was 35.01 M bp in length and annotated with 10396 gene models. 12 putative terpeniod gene clusters were predicted, 12 sesquiterpenes synthase genes belonged to four different groups and two type I PKS (polyketide synthase) gene clusters were identified from F. filiformis genome. The gene number related terpeniod biosynthesis is higher in wild strain (119 genes) than cultivar strain (81 genes) and most of them are up regulated in primodium and fruiting body of the wild strain, while PKS genes are usually up-regulated in the mycelium of wild strain. Moreover, genes encoding UDP-glucose pyrophosphorylase and UDP-glucose dehydrogenase involved in polysaccharide biosynthesis have relative high transcripts both in mycelium and fruiting bodies of F. filiformis.
Conclusions: We identified candidate genes involved in the biosynthesis of polysaccharide and terpenoid bioactive compounds and profiled these genes expression during the development of F. filiformis. This study expends our knowledge for understanding the biology of F. filiformis and provides valuable data for elucidating the secondary metabolism regulation of the special strain of F. filiformis.
The Molecular basis of N-acetylmuramoyl-L-alanine amidase (Rv3915) and Protein Kinase B (PknB) essentiality in Mycobacterium tuberculosis
Authors: Ezechukwu Ifunanya N , Onyekwelu Kenechukwu , Nwokolo Loreta , Turapov Obolbek 
Published: August 2019

Abstract
Background: Protein kinase B (PknB) is critical for the survival of Mycobacterium tuberculosis (M. tuberculosis) in vitro and in hosts. It phosphorylates various enzymes involved in biosynthesis of cell wall and a particular autolysin classified as CwIM or Rv3915 has been recently identified as a PknB substrate. However, in-depth knowledge of this protein is still unknown. The aims of this study were to purify and investigate the activity of Rv3915, as well as monitor the phosphorylation of Rv3915 and the influence of phosphorylation on the activity of this protein. Results: Using the C41 E. coli strain containing a plasmid, with a gene encoding the protein, Rv3915 was either expressed alone or co-expressed with PknB. Sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) was used to observe the amount of Rv3915 purified, anti-poly His and anti-phosphothreonine western blot techniques were used to confirm the presence and phosphorylation of Rv3915 and zymogram assays were run to examine its activity. The results showed that Rv3195 was successfully expressed and was deemed soluble when observed in soluble fraction of E. coli lysate. It was confirmed that Rv3915 is produced as a ~45kDa protein, which does not possess any muralytic activity. However a shorter version of the protein (~25kDA) was active in zymogram, suggesting that Rv3915 is activated by cleavage. Conclusion: Rv3915 was phosphorylated by PknB and phosphorylation apparently controls stability of the protein.
Second-line treatment strategy for urothelial cancer patients who progress or are unfit for cisplatin therapy: a network meta-analysis
Authors: Wang Huitao , Liu Jianhe , Fang Kewei , Ke Changxing , Jiang Yongming , Wang Guang , Yang Tongxin , Chen Tao , Shi Xin 
Published: August 2019

Abstract
Background Second-line treatment for urothelial carcinoma (UC) patients is used if progression or failure after platinum-based chemotherapy occurs or if patients are cisplatin-unfit. However, there is still no widely accepted treatment strategy. We aimed to analyze the effectiveness and safety of second-line treatment strategies for UC patients. Methods The PubMed, Embase, and Cochrane databases were searched for randomized controlled trials (RCTs) that included UC patients who were cisplatin-ineligible or unfit up to April 19, 2019. The primary outcomes were progression-free survival (PFS), overall survival (OS), and objective response rate (ORR). Results Thirteen trials that assessed 3502 UC patients were included. This study divided the network comparisons into three parts. The first part contained studies comparing taxanes and other interventions; the second part assessed investigator’s choice chemotherapy (ICC)-related comparisons; and the third part assessed best support care (BSC). In the OS results of the first part, pembrolizumab (87.5%), ramucirumab plus docetaxel (74.6%), and atezolizumab (71.1%) had a relative advantage. Pembrolizumab also had advantages in ORR and severe adverse effect (SAE) results. Vinflunine and ramucirumab plus docetaxel had a relatively high surface under the cumulative ranking (SUCRA) rank by exploratory cluster analysis. Conclusions This study concluded that atezolizumab and pembrolizumab are superior to other treatments, mainly in OS results, but no treatment confers a significant advantage in PFS. Pembrolizumab still has relative advantages in ORR and SAE results compared to ICC.
Prevalence and determinants of undernutrition among school children of Ethiopia: a systematic review and meta-analysis
Authors: Hailegebreil Tamirat 
Published: August 2019

Abstract
Background: Ethiopia is the second populous countries in Africa where millions of children are affected by malnutrition. Several original works are available online about child malnutrition in Ethiopia. The aim of this study was to summarize the existing epidemiological data on undernutrition and possible determinants among school children in Ethiopia. Methods: We conducted a systematic review using open access articles which report prevalence of stunting and wasting/thinness from PubMed Central, Scopus and Science direct. From 358 articles accessed online, 39 of them were eligible for this meta-analysis. The heterogeneities among studies were evaluated using I2 test and the pooled prevalence was calculated using a random effect model. Results: A total of 24, 716 school children were included in 39 eligible articles for this meta-analysis. The pooled prevalence of stunting and wasting/thinness among school children in Ethiopia were 23.1% (95% CI: 19.0 - 27.0%) and 22% (95% CI: 17% - 26%), respectively. The risk of stunting was increased among the student with meal frequency ≤ 3 times a day (OR= 3.02, 95% CI: 1.90 - 4.14), age > 10 years (OR= 2.16, 95% CI: 1.47 - 2.85) and family size ≥ 5 (OR= 1.88, 95% CI: 1.40 - 2.35). Similarly, the risk of wasting/thinness was increased with male sex (OR= 2.06, 95% CI: 1.54 - 2.58), low family income (OR= 2.16, 95% CI: 1.30 - 3.01) and age > 10 years (OR= 1.78, 95% CI: 0.74 - 2.82). High heterogeneity about the prevalence of stunting and wasting/thinness was observed among studies included in this meta-analysis (I2 > 98.6). Conclusions: This study showed that stunting and wasting/thinness are common nutritional problems in school children in Ethiopia. Large family size, male sex and age above 10 years were consistently associated with stunting and wasting/thinness in children. The finding of this study highlights the importance of new strategies to reduce undernutrition in Ethiopia. Keywords: stunting, wasting, thinness, undernutrition, systematic review, meta-analysis
Determinants of incomplete immunization among children age 12-23 months in Southwest Ethiopia
Authors: G/Mariam Meseret , Ayele Gistane , Shegaze Mulugeta , Wassihun biresaw 
Published: August 2019

Abstract
Background Vaccination is recognized as an important immunization tool which is used in preventing and eradicating communicable diseases. Even though increase in global vaccination coverage, many children in the world particularly in low resource countries still are left unvaccinated and it remains to be a public health problem.Objective To identify determinants of incomplete vaccination among children 12-23 months of age in Semen Bench district, Bench Maji Zone, Southwest Ethiopia, 2018.Methods Community based case-control study design was employed. By using simple random sampling a total 312 children aged between 12-23 months from households were selected from 7 kebeles in Semen Bench district, Bench Maji Zone southern regional state. Data was entered into Epi Inof version 7 and was exported into SPSS version 21.0 for analysis. . Both bivariate and multivariate logistic regression analysis were performed to identify associated factors. P values <0.05 with 95% confidence level were used to declare statistical significance.Results A total of 312 respondents participated in the study with a response rate of 100%. The multivariable analysis indicated that respondents having no antenatal visit [AOR=8.3(95%CI; 1.87-36.91], giving birth in home delivery AOR=4.5(95%CI; 1.411-14.27], and respondents having no postnatal visit AOR=4.2(95%CI; 1.67-10.41], were some of the variables continued to have statistically significant predictors of incomplete vaccination.Conclusion This study was identified some of the factors associated with incomplete vaccination. In order to decrease neonatal mortality resulted from communicable diseases Strengthen antenatal care visit and prompting postnatal service and institutional delivery is very important in addition to this it is crucial that, local programmatic intervention should be strengthened to promote awareness of the community on the importance of Vaccination.
Safety and Efficacy of 25G vitrectomy surgery for malignant glaucoma in pseudophakia
Authors: you fei 
Published: August 2019

Abstract
Background: malignant glaucoma after cataract surgery is still one of the serious complications, if not handled properly，it may lead to serious consequences. It is notoriously difficult to treat. 25G vitrectomy was performed to evaluate the safety and efficacy for the treatment of malignant glaucoma in pseudophakia. Methods: This is a retrospective, comparative case series study. A total of 20 eyes of 20 patients with malignant glaucoma after phacoemulsification were analyzed retrospectively in The First Affiliated Hospital Of Anhui Medical University from May 2015 to January 2018. All Medical Data including the best corrected visual acuity (BCVA), Change of intraocular pressure (IOP), the length of eye axis, and the depth of anterior chamber were recorded. SPSS 17.0 statistical software was used for analysis .Before surgery, the best corrected visual acuity (BCVA) was 1.8±0.6. The intraocular pressure was between 18-57mmHg, with an average of 35.2±10.4mmHg.The depth of anterior chamber was between 0.9-1.9mm, with an average of 1.3±0.2mm.The length of eye axis was 19.7-22.5mm,with an average of 20.6±0.5mm.All the patients were accomplished with 25G vitrectomy. Besides, anterior chamber inflammatory reaction and other complications were also observed postoperation. Results: The patients were followed up for 6-12 months with an average of 9 months. BCVA at the last follow up improved to 0.8±0.1, and there was significant difference compared to that before operation (P<0.01).IOP was from 12-19mmHg, an average of 16.1±2.5mmHg, there was significant difference compared to that before operation (t=7.6, p<0.01).Only one patient occurred low IOP (6mmHg) after surgery, IOP returned to normal level (14mmHg) after conservative treatment. No serious complications including corneal endothelium decompensation, intraocular lens (IOL) capture, intraocular hemorrhage, endophthalmitis and uncontrolled IOP were observed. Conclusions: 25G minimally invasive vitrectomy can treat malignant glaucoma after cataract surgery safely and effectively
The clinicopathologic characteristics of Immunoglobulin A nephropathy related to C3 deposits
Authors: Wu Lingzhi , Liu Di , Chen Guochun , Liu Yu , You Jing , Tang Xiaofang , Xia Ming , Zhu Xuejing , Liu Hong 
Published: August 2019

Abstract
Background

C3 deposits are widely detected in patients with immunoglobulin A nephropathy (IgAN). However, the relationship between the location and intensity of the C3 deposition and prognosis of IgAN is uncertain.

Methods

244 patients diagnosed with IgAN were enrolled in our study during 2010- 2016. Patients were divided into groups according to the location of C3 deposits: mesangial area only versus mesangial area and glomerular capillary loops. We also divided those patients into 4 groups according to the intensity of C3 deposition at the base of location. Renal outcomes were end-stage renal disease(ESRD)or a ≥50% reduction in estimated glomerular filtration rate(eGFR), performed by Kaplan–Meier analysis and Cox proportional hazards models.

Results

Among the 244 recruited patients with IgAN, 139(56.97%) were female. Compared to patients with C3 deposition in the mesangial area only, those with C3 deposition in the mesangial area and glomerular capillary loops had significantly older, lower levels of serum albumin and eGFR, higher levels of uric acid (UA), severe proteinuria, more intense C3 deposits, were more prone to receive corticosteroids and immunosuppression. There were no other significant differences between C3 deposition in the mesangial area only (≤2+ in intensity) group and C3 deposition in the mesangial area and glomerular capillary loops (≤2+ in intensity) group except for age. Patients in C3 deposition in the mesangial area and glomerular capillary loops (>2+ in intensity) group were associated with lower levels of serum albumin, serum IgG and eGFR, higher levels of serum IgM, UA and blood urea nitrogen (BUN),heavier proteinuria and higher interstitial fibrosis/tubular atrophy scores than C3 deposition in the mesangial area deposits only(>2+ in intensity) group. After a median follow-up of 41.86 months,28(11.48%) patients reached the renal outcomes. Higher intensity of C3 deposition in the mesangial area and glomerular capillary loops (95%CI:1.262-76.832, p=0.029) remained an independent risk factor of renal outcomes by multivariate Cox proportional hazards models.

Conclusions

IgAN patients who had glomerular capillary loops C3 deposits had worse clinical outcomes. Furthermore, the effect of the C3 deposition site on IgA nephropathy is affected by the intensity of C3 deposits.