bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface passivation engineering strategy to fully-inorganic cubic CsPbI3 perovskites for high-performance solar cells
Authors: Li Bo , Zhang Yanan , Fu Lin , Yu Tong , Zhou Shujie , Zhang Luyuan , Yin Longwei 
Published: March 2018

AbstractOwing to inevitable thermal/moisture instability for organic–inorganic hybrid perovskites, pure inorganic perovskite cesium lead halides with both inherent stability and prominent photovoltaic performance have become research hotspots as a promising candidate for commercial perovskite solar cells. However, it is still a serious challenge to synthesize desired cubic cesium lead iodides (CsPbI3) with superior photovoltaic performance for its thermodynamically metastable characteristics. Herein, polymer poly-vinylpyrrolidone (PVP)-induced surface passivation engineering is reported to synthesize extra-long-term stable cubic CsPbI3. It is revealed that acylamino groups of PVP induce electron cloud density enhancement on the surface of CsPbI3, thus lowering surface energy, conducive to stabilize cubic CsPbI3 even in micrometer scale. The cubic-CsPbI3 PSCs exhibit extra-long carrier diffusion length (over 1.5 μm), highest power conversion efficiency of 10.74% and excellent thermal/moisture stability. This result provides important progress towards understanding of phase stability in realization of large-scale preparations of efficient and stable inorganic PSCs.
A non-conserved amino acid variant regulates differential signalling between human and mouse CD28
Authors: Porciello Nicla , Grazioli Paola , Campese Antonio F. , Kunkl Martina , Caristi Silvana , Mastrogiovanni Marta , Muscolini Michela , Spadaro Francesca , Favre Cédric , Nunès Jacques A. , Borroto Aldo , Alarcon Balbino , Screpanti Isabella , Tuosto Loretta 
Published: March 2018

AbstractCD28 superagonistic antibodies (CD28SAb) can preferentially activate and expand immunosuppressive regulatory T cells (Treg) in mice. However, pre-clinical trials assessing CD28SAbs for the therapy of autoimmune diseases reveal severe systemic inflammatory response syndrome in humans, thereby implying the existence of distinct signalling abilities between human and mouse CD28. Here, we show that a single amino acid variant within the C-terminal proline-rich motif of human and mouse CD28 (P212in human vs. A210in mouse) regulates CD28-induced NF-κB activation and pro-inflammatory cytokine gene expression. Moreover, this Y209APP212sequence in humans is crucial for the association of CD28 with the Nck adaptor protein for actin cytoskeleton reorganisation events necessary for CD28 autonomous signalling. This study thus unveils different outcomes between human and mouse CD28 signalling to underscore the importance of species difference when transferring results from preclinical models to the bedside.
Thyroid Dysfunction, Neurological Disorder and Immunosuppression as the Consequences of Long-term Combined Stress
Authors: Zhang Jingping , Huang Jingjing , Aximujiang Kasimujiang , Xu Chenbo , Ahemaiti Abulaiti , Wu Guixia , Zhong Li , Yunusi Kurexi 
Published: March 2018

AbstractStress is a powerful modulator of neuroendocrine, behavioral, and immunological functions. So far, the molecular mechanisms of response to stressors still remain elusive. In the current study, after 10 days of repeated chronic stress (hot-dry environment and electric foot-shock), a murine model of combined-stress (CS) was created in the SPF Wistar rats. Meanwhile, we established an ulcerative-colitis (UC) rat model induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol enema according to previous studies. The blood, hypothalamus, and colon tissues of these rats from CS, normal control (NC), UC and sham (SH) groups, were collected for further investigations. Comparing to the NC group, the serum levels of T3, T4, fT3 and fT4 were obviously decreased in the CS group after chronic stress, indicating that thyroid dysfunction was induced by long-term combined stress. Moreover, the application of RNA-seq and subsequent analyses revealed that neurological disorder and immunosuppression were also caused in the hypothalamus and colon tissues, respectively. Comparing with SH group, besides the induced colon inflammation, thyroid dysfuntion and neurological disorder were also produced in the UC group, suggesting that hypothalamic-pituitary-thyroid (HPT) axis and gastrointestinal system might not function in isolation, but rather, have intricate crosstalks.
Mutant Best1 Expression and Impaired Phagocytosis in an iPSC Model of Autosomal Recessive Bestrophinopathy
Authors: Marmorstein Alan D. , Johnson Adiv A. , Bachman Lori A. , Andrews-Pfannkoch Cynthia , Knudsen Travis , Gilles Benjamin J. , Hill Matthew , Gandhi Jarel K. , Marmorstein Lihua Y. , Pulido Jose S. 
Published: March 2018

AbstractAutosomal recessive bestrophinopathy (ARB) is caused by mutations in the gene BEST1 which encodes bestrophin 1 (Best1), an anion channel expressed in retinal pigment epithelial (RPE) cells. It has been hypothesized that ARB represents the human null phenotype for BEST1 and that this occurs due to nonsense mediated decay (NMD). To test this hypothesis, we generated induced pluripotent stem cells (iPSCs) from a patient with ARB and her parents. After differentiation to retinal pigment epithelial (iPSC-RPE) cells, both BEST1 mRNA and Best1 protein expression were compared to controls. BEST1 mRNA expression levels, determined by quantitative PCR, were similar in ARB iPSC-RPE, parental cells, and genetically unrelated controls. Western blotting revealed that CRALBP and RPE65 were expressed within the range delineated by unrelated controls in iPSC-RPE from the ARB donor and her parents. Best1 protein was detected in different clones of ARB iPSC-RPE, but at reduced levels compared to all controls. When tested for the ability to phagocytose photoreceptor outer segments, ARB iPSC-RPE exhibited impaired internalization. These data suggest that impaired phagocytosis is a trait common to the bestrophinopathies. Furthermore, ARB is not universally the result of NMD and ARB, in this patient, is not due to the absence of Best1.
Neonatal nephron loss during active nephrogenesis – detrimental impact with long-term renal consequences
Authors: Menendez-Castro Carlos , Nitz Dörte , Cordasic Nada , Jordan Jutta , Bäuerle Tobias , Fahlbusch Fabian B. , Rascher Wolfgang , Hilgers Karl F. , Hartner Andrea 
Published: March 2018

AbstractNeonatal nephron loss may follow hypoxic-ischemic events or nephrotoxic medications. Its long-term effects on the kidney are still unclear. Unlike term infants, preterm neonates less than 36 weeks gestational age show ongoing nephrogenesis. We hypothesized that nephron loss during nephrogenesis leads to more severe renal sequelae than nephron loss shortly after the completion of nephrogenesis. Rats show nephrogenesis until day 10 of life resembling the situation of preterm infants. Animals were uninephrectomized at day 1 (UNX d1) resulting in nephron reduction during nephrogenesis and at day 14 of life (UNX d14) inducing nephron loss after the completion of nephrogenesis. 28 days after uninephrectomy the compensatory renal growth was higher in UNX d1 compared to UNX d14. Nephrin was reduced and collagen deposition increased in UNX d1. At 1 year of age, glomerulosclerosis and markers of tubulointerstitial damage were most prevalent in UNX d1. Moreover, the number of desmin-positive podocytes was higher and nephrin was reduced in UNX d1 indicating podocyte damage. Infiltration of inflammatory cells was heightened after UNX d1. Uninephrectomized animals showed no arterial hypertension. We conclude that neonatal nephron loss during active nephrogenesis leads to more severe glomerular and tubulointerstitial damage, which is not a consequence of compensatory arterial hypertension.
Recent high-resolution Antarctic ice velocity maps reveal increased mass loss in Wilkes Land, East Antarctica
Authors: Shen Qiang , Wang Hansheng , Shum C. K. , Jiang Liming , Hsu Hou Tse , Dong Jinglong 
Published: March 2018

AbstractWe constructed Antarctic ice velocity maps from Landsat 8 images for the years 2014 and 2015 at a high spatial resolution (100 m). These maps were assembled from 10,690 scenes of displacement vectors inferred from more than 10,000 optical images acquired from December 2013 through March 2016. We estimated the mass discharge of the Antarctic ice sheet in 2008, 2014, and 2015 using the Landsat ice velocity maps, interferometric synthetic aperture radar (InSAR)-derived ice velocity maps (~2008) available from prior studies, and ice thickness data. An increased mass discharge (53 ± 14 Gt yr−1) was found in the East Indian Ocean sector since 2008 due to unexpected widespread glacial acceleration in Wilkes Land, East Antarctica, while the other five oceanic sectors did not exhibit significant changes. However, present-day increased mass loss was found by previous studies predominantly in west Antarctica and the Antarctic Peninsula. The newly discovered increased mass loss in Wilkes Land suggests that the ocean heat flux may already be influencing ice dynamics in the marine-based sector of the East Antarctic ice sheet (EAIS). The marine-based sector could be adversely impacted by ongoing warming in the Southern Ocean, and this process may be conducive to destabilization.
Effect of tillage and crop residue on soil temperature following planting for a Black soil in Northeast China
Authors: Shen Yan , McLaughlin Neil , Zhang Xiaoping , Xu Minggang , Liang Aizhen 
Published: March 2018

AbstractCrop residue return is imperative to maintain soil health and productivity but some farmers resist adopting conservation tillage systems with residue return fearing reduced soil temperature following planting and crop yield. Soil temperatures were measured at 10 cm depth for one month following planting from 2004 to 2007 in a field experiment in Northeast China. Tillage treatments included mouldboard plough (MP), no till (NT), and ridge till (RT) with maize (Zea mays L.) and soybean (Glycine max Merr.) crops. Tillage had significant effects on soil temperature in 10 of 15 weekly periods. Weekly average NT soil temperature was 0–1.5 °C lower than MP, but the difference was significant (P < 0.05) only in 2007 when residue was not returned in MP the previous autumn. RT showed no clear advantage over NT in increasing soil temperature. Higher residue coverage caused lower soil temperature; the effect was greater for maize than soybean residue. Residue type had significant effect on soil temperature in 9 of 15 weekly periods with 0–1.9 °C lower soil temperature under maize than soybean residue. Both tillage and residue had small but inconsistent effect on soil temperature following planting in Northeast China representative of a cool to temperate zone.
Genetic variations in the fusion protein of respiratory syncytial virus isolated from children hospitalized with community-acquired pneumonia in China
Authors: Chen Xiangpeng , Xu Baoping , Guo Jiayun , Li Changchong , An Shuhua , Zhou Yunlian , Chen Aihuan , Deng Li , Fu Zhou , Zhu Yun , Liu Chunyan , Xu Lili , Wang Wei , Shen Kunling , Xie Zhengde 
Published: March 2018

AbstractTo identify the variations in fusion (F) protein gene of RSV in China, a molecular epidemiological study was conducted. A total of 553 RSV positive specimens were collected from 2338 pediatric patients hospitalized with community-acquired pneumonia during a multi-center study conducted during 2014–2016. A total of 252 samples (183 RSV A, 69 RSV B) were selected for F gene sequencing, and analyzed together with 142 F gene sequences downloaded from GenBank. The result showed that all the Chinese RSV A and RSV B strains could be divided respectively into three branches. Compared with RSV A/B prototype sequences respectively, there were significant amino acid (AA) mutations at multiple antigenic sites. For RSV A, changes were found at AA residues 122, 124, 125, 276 and 384, and for RSV B at AA residues 45, 116, 125, 172, 173 and 202. Variations in human histocompatibility leukocyte antigen-restricted CTL epitopes were also observed. In total, 56 amino acid differences for the complete F protein were found between the RSV A and B groups in China, while several mutations were only found in the RSV B strains during 2015–2016. The RSV F gene is relatively conserved in China, however, limited mutations are still occurring with time.
Sexual transmission of Zika virus enhances in utero transmission in a mouse model
Authors: Duggal Nisha K. , McDonald Erin M. , Ritter Jana M. , Brault Aaron C. 
Published: March 2018

AbstractZika virus (ZIKV) is an emerging mosquito-borne virus that can cause ZIKV congenital syndrome when a pregnant woman is infected. Sexual transmission has also been described for ZIKV, though the relationship between sexual transmission and vertical transmission has not been investigated. Here, viral dissemination to the female reproductive tract and fetuses was assessed in immunodeficient (AG129) female mice that were exposed to ZIKV by subcutaneous (s.c.) inoculation, intravaginal (ivag.) inoculation, or sexual transmission from infected male AG129 mice. Pregnant females had significantly increased ZIKV dissemination to the female reproductive tract compared to non-pregnant females when exposed by s.c. or ivag. inoculation. Sexual transmission resulted in significantly greater morbidity and mortality in females and higher ZIKV titers in the female reproductive tract than s.c. or ivag. inoculation. Ovaries from females infected sexually contained ZIKV RNA within the ovarian follicles. Furthermore, ZIKV titers were significantly higher in fetuses from dams exposed sexually compared to fetuses from dams exposed by s.c. or ivag. inoculation. These results demonstrate that sexual transmission enhances dissemination of ZIKV to the female reproductive tract and developing fetuses in a mouse model.
Performing radiosynthesis in microvolumes to maximize molar activity of tracers for positron emission tomography
Authors: Sergeev Maxim , Lazari Mark , Morgia Federica , Collins Jeffrey , Javed Muhammad Rashed , Sergeeva Olga , Jones Jason , Phelps Michael E. , Lee Jason T. , Keng Pei Yuin , van Dam R. Michael 
Published: March 2018

AbstractPositron emission tomography (PET) is a molecular diagnostic imaging technology to quantitatively visualize biological processes in vivo. For many applications, including imaging of low-tissue density targets (e.g., neuroreceptors), imaging in small animals, and evaluation of novel tracers, the injected PET tracer must be produced with high molar activity to ensure low occupancy of biological targets and avoid pharmacologic effects. Additionally, high molar activity is essential for tracers with lengthy syntheses or tracers transported to distant imaging sites. Here we show that radiosynthesis of PET tracers in microliter volumes instead of conventional milliliter volumes results in substantially increased molar activity, and we identify the most relevant variables affecting this parameter. Furthermore, using the PET tracer [18F]fallypride, we illustrate that molar activity can have a significant impact on biodistribution. With full automation, microdroplet platforms could provide a means for radiochemists to routinely, conveniently, and safely produce PET tracers with high molar activity.
Hyperthermostable cube-shaped assembly in water
Authors: Zhan Yi-Yang , Ogata Kazuho , Kojima Tatsuo , Koide Takuya , Ishii Kentaro , Mashiko Takako , Tachikawa Masanori , Uchiyama Susumu , Hiraoka Shuichi 
Published: March 2018

AbstractProteins in hyperthermophiles exhibit extremely high thermal stability unlike general proteins. These thermostable proteins are stabilized by weak molecular interactions such as hydrogen bonding, charge interactions and van der Waals (vdW) interactions, along with the hydrophobic effect. An in-depth understanding of the stabilization mechanisms will enable us to rationally design artificial molecules with very high thermal stability. Here we show thermally stable supramolecular assemblies composed of six identical amphiphilic molecules having an indented hydrophobic surface, held together by weak intermolecular interactions (vdW and cation-π interactions) and the hydrophobic effect in water. The disassembly temperature of one of the assemblies is over 150 °C, which is higher than that of the most hyperthermophilic protein reported to date (PhCutA1). Study of the relationship between the structure of the components and the stability of the assemblies indicates that the hyperthermostability is achieved only if all the weak interactions and the hydrophobic effect work cooperatively.
Versatile bimetallic lanthanide metal-organic frameworks for tunable emission and efficient fluorescence sensing
Authors: Su Yan , Yu Junhong , Li Youbing , Phua Soo Fiona Zeng , Liu Guofeng , Lim Wei Qi , Yang Xiaozhan , Ganguly Rakesh , Dang Cuong , Yang Chaolong , Zhao Yanli 
Published: March 2018

AbstractDeveloping novel lanthanide metal-organic frameworks (Ln-MOFs) to rapidly and reliably differentiate both metal ions in solution and volatile organic compounds (VOCs) in vapor is highly challenging. Here, we describe versatile Eu3+/Tb3+-MOFs based on a flexible ligand. It is noteworthy that the film fabricated using bimetallic Eu0.47Tb0.63-MOF and polyvinyl alcohol could serve as an easy and convenient luminescent platform for distinguishing different metal ions and VOCs. The luminescent film exhibits notable fingerprint correlation between the metal ions/VOCs and the emission intensity ratio of Eu3+/Tb3+ ions in Ln-MOFs. As a result, the bimetallic Ln-MOFs show fast recognition of Fe3+ ion with a response time of <10 s, and can effectively probe styrene vapor within 4 min. Since the developed Ln-MOF film is stable and reliable, this work presents a promising strategy to explore luminescent platforms capable of effectively sensing different metal ions and VOCs.
Wide‐band signal‐interference duplexer with contiguous single/dual‐band channels and its application to quasi‐absorptive bandpass filters
Authors: Gómez‐García Roberto , Muñoz‐Ferreras José‐María , Feng Wenjie , Psychogiou Dimitra 
Published: March 2018

A type of wide‐band signal‐interference planar duplexer with single‐ and dual‐band channels is presented. It exploits transversal‐signal‐interference‐section‐based channels with contiguous single‐ and dual‐passband filtering transfer functions. Furthermore, by ending the dual‐band channel in a reference‐impedance resistor, a wide‐band bandpass filter (BPF) with quasi‐absorptive stopbands is realised. As experimental validation, microstrip prototypes of a duplexer and a two‐stage quasi‐absorptive BPF that operate in the 1.6–4.4 GHz range are built and tested.
A Review of the Traditional Uses, Phytochemistry and Biological Activities of the Genus Santolina
Authors: Tundis Rosa , Loizzo Monica 
Published: March 2018

AbstractThe genus Santolina is a taxonomically complex group of plant species widely distributed in the Mediterranean flora and used in traditional medicine since ancient times for their biological properties, including antimicrobial, anti-inflammatory, antispasmodic, digestive, and analgesic activities. Phytochemical investigations of Santolina species have revealed the presence of terpenoids as the main bioactive constituents of the genus. Coumarins and flavonoids were also identified. This review deals, for the first time, with information on the traditional uses, chemical profile, and biological properties of plants of the genus Santolina in order to provide input for future research prospects.
Essentiality and function of WalK/WalR two-component system: the past, present, and future of research*
Authors: Takada Hiraku , Yoshikawa Hirofumi 
Published: March 2018

Abstract
The WalK/WalR two-component system (TCS), originally identified in Bacillus subtilis, is very highly conserved in gram-positive bacteria, including several important pathogens. The WalK/WalR TCS appears to be involved in the growth of most bacterial species encoding it. Previous studies have indicated conserved functions of this system, defining this signal transduction pathway as a crucial regulatory system for cell wall metabolism. Because of such effects on essential functions, this system is considered a potential target for anti-infective therapeutics. In this review, we discuss the role of WalK/WalR TCS in different bacterial cells, focusing on the function of the genes in its regulon as well as the variations in walRK operon structure, its auxiliary proteins, and the composition of its regulon. We also discuss recent experimental data addressing its essential function and the potential type of signal being sensed by B. subtilis. This review also focuses on the potential future research.
Testing Representational Advantage in the Argentine Supreme Court
Authors: Muro Sergio , Chehtman Alejandro , Méndez Jorge Silva , Durán Nelson Amaya 
Published: March 2018

AbstractEven if party capability theory has been well documented, parsing out the reasons why “haves” come out ahead has been challenging. Our study takes advantage of the Argentine Supreme Court’s power to dismiss appeals because they contain formal errors to ascertain the existence of representational advantage. We show that representational advantage plays a significant role, as individual appellants represent a larger proportion of appeals rejected on formal grounds than of those analyzed on their merits. In addition, certain areas of law where asymmetrical capability is prevalent and consistent, particularly labor law, are significantly overrepresented in appeals rejected on formal grounds.
Infrasound from the 2009 and 2017 DPRK rocket launches
Authors: Evers L G , Assink J D , Smets P SM 
Published: March 2018

SummarySupersonic rockets generate low-frequency acoustic waves, that is, infrasound, during the launch and re-entry. Infrasound is routinely observed at infrasound arrays from the International Monitoring System, in place for the verification of the Comprehensive Nuclear-Test-Ban Treaty. Association and source identification are key elements of the verification system. The moving nature of a rocket is a defining criterion in order to distinguish it from an isolated explosion. Here, it is shown how infrasound recordings can be associated, which leads to identification of the rocket. Propagation modelling is included to further constrain the source identification. Four rocket launches by the Democratic People's Republic of Korea in 2009 and 2017 are analysed in which multiple arrays detected the infrasound. Source identification in this region is important for verification purposes. It is concluded that with a passive monitoring technique such as infrasound, characteristics can be remotely obtained on sources of interest, that is, infrasonic intelligence, over 4500+ km.