bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fetal and organ development at gestational days 45, 90, 135 and at birth of lambs exposed to under- or over-nutrition during gestation1,2,3
Authors: Pillai S. M. , Jones A. K. , Hoffman M. L. , McFadden K. K. , Reed S. A. , Zinn S. A. , Govoni K. E. 
Published: December 2018

Abstract
To determine the effects of poor maternal nutrition on offspring body and organ growth during gestation, pregnant Western White-faced ewes (n = 82) were randomly assigned into a 3 × 4 factorial treatment structure at d 30.2 ± 0.2 of gestation (n = 5 to 7 ewes per treatment). Ewes were individually fed 100% (control), 60% (restricted) or 140% (over) of NRC requirements for TDN. Ewes were euthanized at d 45, 90 or 135 of gestation or underwent parturition (birth) and tissues were collected from the offspring (n = 10 to 15 offspring per treatment). Offspring from control, restricted and overfed ewes are referred to as CON, RES and OVER, respectively. Ewe data were analyzed as a completely randomized design and offspring data were analyzed as a split-plot design using PROC MIXED. Ewe BW did not differ at d 30 (P ≥ 0.43), however restricted ewes weighed less than overfed and overfed were heavier than controls at d 45, and restricted weighed less and overfed were heavier than controls at d 90 and 135 and birth (P ≤ 0.05). Ewe BCS was similar at d 30, 45 and 90 (P ≤ 0.07), however restricted ewes scored lower than control at d 135 and birth (P ≤ 0.05) and over ewes scored higher than control at d 135 (P ≤ 0.05) but not at birth (P = 0.06). A maternal diet by day of gestation interaction indicated that at birth the body weight (BW) of RES offspring was less than CON and OVER (P ≤ 0.04) and heart girth of RES was smaller than CON and OVER (P ≤ 0.004). There was no interaction of maternal diet and day of gestation on crown-rump, fetal, or nose occipital length, or orbit or umbilical diam. (P ≥ 0.31). A main effect of maternal diet indicated that the RES crown-rump length was shorter than CON and OVER (P ≤ 0.05). An interaction was observed for liver, kidney and renal fat (P ≤ 0.02). At d 45 the liver of RES offspring was larger than CON and OVER (P ≤ 0.002), but no differences observed at d 90, 135 or birth (P ≥ 0.07). At d 45, the kidneys of OVER offspring were larger than CON and RES (P ≤ 0.04), but no differences observed at d 90, 135 or birth (P ≥ 0.60). At d 135, OVER had more perirenal fat than CON and RES (P ≤ 0.03), and at birth RES had more perirenal fat than CON and OVER (P ≤ 0.04). There was no interaction observed for offspring heart weight, length or width, kidney length, adrenal gland weight, loin eye area or rib width (P ≥ 0.09). In conclusion, poor maternal nutrition differentially alters offspring body size and organ growth depending on the stage of gestation.
A summary review of carcass cutability data comparing primal value of immunologically and physically castrated barrows
Authors: Harsh B. N. , Cowles B. , Johnson R. C. , Pollmann D. S. , Schroeder A. L. , Dilger A. C. , Boler D. D. 
Published: December 2018

Abstract
The objectives were to 1) assess cutability, quality, and value of carcasses from immunologically castrated (IC) barrows compared with carcasses from physically castrated (PC) barrows and 2) evaluate the effect of hot carcass weight (HCW) on cutability and value of IC barrows summarizing U.S. data. Lean cutting yield (LCY) was defined as: LCY = [(whole ham + trimmed loin + Boston butt + picnic + spareribs)/chilled side wt] x 100. Carcass cutting yield (CCY) was determined using the following equation: CCY = [(lean cutting yield components + natural fall belly)/chilled side wt] x 100. To evaluate the effects of HCW of IC barrows on carcass cutting yields, IC barrows were grouped by HCW: light ( &lt; 90.9 kg), average (90.9–97.7 kg), or heavy ( &gt; 97.7 kg). Differences in the value of the carcass components for IC and PC barrow carcasses were calculated using a 5 yr average of meat prices from the USDA Agriculture Marketing Service and the carcass cutting yield estimates generated from this summary. Data were analyzed using the MIXED procedure of SAS with fixed effects of Improvest treatment or HCW group. Study was included as a random effect. This review allowed for a summarization of the treatment averages of 851 IC and PC barrow carcasses. Lean cutting yield of IC barrows was 1.41 units greater (P &lt; 0.0001) than PC barrows (70.97 vs. 69.56%). Similarly, CCY of IC barrows was 1.29 units greater (P &lt; 0.001) compared with PC barrows (87.27 vs. 85.98%). As HCW of IC barrows increased, both CCY and LCY declined (P &lt; 0.01), with light IC barrow carcasses having a 1.43 unit advantage in CCY compared with heavy IC barrow carcasses (P &lt; 0.01). Natural fall bellies of PC barrows comprised a greater (P &lt; 0.05) percentage of side weight than those from IC barrows (15.81 vs. 15.50%). A reduction in belly primal value was confirmed by a 3.43 unit reduction in the commercial bacon slicing yields of IC barrows. However, belly yield and slicing yield differences were minimized when IC barrows were marketed at a heavier weight. Using carcass cutout estimates determined in the summary as the foundation for value calculations, lean cuts of IC barrow carcasses were worth $2.66 to $3.80 more than PC barrow carcasses. Therefore, after adjustment for the reduction in belly primal value, the primal value of an IC barrow carcass was $2.08 to $3.13 greater than a PC barrow carcass.
Effects of exogenous phytase supplementation on phosphorus metabolism and digestibility of beef cattle
Authors: Long C. J. , Kondratovich L. B. , Westphalen M. F. , Stein H. H. , Felix T. L. 
Published: December 2018

Abstract
Objectives were to determine interactions between phytase inclusion and dietary P concentration on P utilization by beef cattle fed a starch-based diet. Six ruminally-fistulated steers (BW = 750 ± 61 kg) were allotted to a 6 × 6 Latin square design with a 3 × 2 factorial arrangement of treatments. Factors included phytase inclusion, at 0, 500, or 2,000 phytase units (FTU)/kg of diet DM, and dietary P concentrations, at 0.10% and 0.30% of total diet DM. Feed ingredients, fecal samples, and orts were composited within period, lyophilized and ground. Samples were analyzed for NDF, ADF, CP, fat, ash, total P, and other minerals. Data were analyzed using the MIXED procedure of SAS with animal as the experimental unit. The CORR procedure was used to compare blood and urinary P concentrations. There were no treatment interactions (P ≥ 0.30) for any parameter measured. There were no main effects (P ≥ 0.45) of phytase inclusion on DMI, total fecal output, apparent DM digestibility, water intake, or urinary output. Steers fed 0.10% P had decreased (P &lt; 0.01) DMI and total fecal output, but increased (P &lt; 0.01) apparent DM digestibility compared with steers fed 0.30% P. Although N intake and retention were not affected by treatment, steers fed the 0.10% P diet tended (P = 0.10) to absorb more N compared with steers fed 0.30% P; and, steers fed the 0.10% P diets excreted more N in the urine (P = 0.02) and less N in the feces (P &lt; 0.01) compared with steers fed the 0.30% P diets. Steers fed the 0.10% P diets also consumed 70.1% less (P &lt; 0.01) total P each day, and excreted 51.9% less (P &lt; 0.01) P in feces and 94.6% less P in the urine (P &lt; 0.01) compared with steers fed 0.30% P. Excretion of water-soluble P in the feces was greater (P &lt; 0.01) on a g/d basis for steers fed 0.30% P when compared with steers fed 0.10% P. However, the proportion of total fecal P excreted as water-soluble P increased (P &lt; 0.05) by 23.0% in steers fed 0.10% P compared with steers fed 0.30% P, regardless of phytase inclusion level. There was no effect of dietary phytase concentration on blood or urinary (P ≥ 0.27) P concentrations. Blood P concentration was positively correlated (r = 0.60; P &lt; 0.01) to urinary P concentration when steers were fed 0.10% P; however, when steers were fed 0.30% P, there was no correlation (r = 0.36; P = 0.16) between blood and urine P. Regardless of dietary P concentration, phytase supplementation did not increase calculated P absorption or retention.
Response to a Salmonella Typhimurium challenge in piglets supplemented with protected sodium butyrate or Bacillus licheniformis: effects on performance, intestinal health and behavior1,2
Authors: Barba-Vidal E. , Roll V. F. B. , Castillejos L. , Guerra-Ordaz A. A. , Manteca X. , Mallo J. J. , Martín-Orúe S. M. 
Published: December 2018

Abstract
Salmonella spp. is one of the worldwide leading causes of food-borne illnesses for which the inclusion of probiotics or organic acids in animal feeds can be useful control methods. Experimental models are utilized to test the efficacy of strategies against pathogens, but they exhibit limitations which may preclude finding sensible evaluation parameters. The objective of this work is to evaluate the efficacy of 2 different feed additives; a Bacillus licheniformis based probiotic and a protected sodium butyrate (SB) salt, using an experimental model of salmonellosis and, second, to explore if behavior analysis can be used as a sensible evaluation tool for additives evaluation. A total of 78 piglets weaned at 24 d, 8.3 kg BW, were used. Seventy-two were placed in 3 rooms of 8 pens (3 animals/pen) with evenly distributed treatments (n = 8): CON, control group with plain diet; PRO, plain diet with 1 kg/t of Proporc (109 cfu of B. licheniformis/kg of feed), and BUT, plain diet with 3 kg/t of Gustor BP70 (2.1 g of partially protected SB salt/kg of feed). Remaining piglets (n = 6) were separated and used as a challenge negative control. The experiment lasted 16 d. After 1 wk of adaptation, animals were challenged with 5 × 108 cfu of Salmonella Typhimurium. One pig per pen was euthanized and sampled at d 4 and 8 post-inoculation (PI). There were no significant differences among treatments for ADFI, ADG, G:F, rectal temperature, fecal consistency, pH, ammonia, short-chain fatty acids and lactic acid concentrations, cytokine TNF-α, Pig-MAP acute-phase proteins and histological parameters. However, both products were equally able to reduce colonization and shedding of Salmonella (P = 0.016 for PRO and BUT vs. CON). In addition, PRO treatment had a positive effect on behavioral displays, particularly exploring (P &lt; 0.05 vs. CON), feeding (P &lt; 0.05 vs. CON and BUT) and other active behaviors (P &lt; 0.05 vs. CON and BUT) in the morning period (0830 to 1030 h). In the afternoon (1400 to 1600 h), the challenge effect was most significant. Pigs were less active after the challenge (P &lt; 0.001), with a decrease in positive contacts (P = 0.004), exploration (P &lt; 0.001) and feeding behaviors (P &lt; 0.001) on d 3 PI, in comparison with before the challenge. Accordingly, many lying conducts increased at d 3 PI (P &lt; 0.05). In conclusion, both treatments had positive effects against Salmonella, and behavior analysis appears to be a sensible tool to be considered.
Use of a novel oleaginous microorganism as a potential source of lipids for weanling pigs1,2
Authors: Carroll J. A. , Schmidt T. B. , Callaway T. R. , Wilson J. G. , Donaldson J. R. 
Published: December 2018

AbstractWeanling pigs are at risk of succumbing to illness due to an immature immune system and insufficient supply of available energy at the time of weaning. This study was aimed at determining whether oleaginous bacteria could serve as a source of lipids to weanling pigs. Weanling pigs were provided a daily dose of 1×109 colony fomring unit (CFU) = kg−1 of the novel oleaginous Enterobacter cloacae strain JD6301 or JD8715 (which is a variant form of JD6301 capable of producing extracellular triglycerides) via oral gavage for 5 d. Serum was collected every 6 h and intestinal samples were collected at 6 d. Providing pigs with JD6301 or JD8715 significantly increased serum concentrations of triglycerides and non-esterified fatty acids (NEFA) within 72 h. Additionally, the JD6301 and JD8715 strains were able to survive within the gastrointestinal tract throughout the duration of the study. These results suggest that providing Enterobacter cloacae can increase the serum lipids in the pigs, thus potentially providing an additional source of energy to animals during times of stress. This could potentially help improve the metabolic response of animals during times of stress.
A systematic review and meta-analysis of tenderness metrics in control groups used in comparative nutrition experiments1,2
Authors: Powell M. J. , Yuan C. , Dzikamunhenga R. S. , Tarté R. , Huff-Lonergan E. , Lonergan S. M. , O'Connor A. M. 
Published: December 2018

Abstract
This review reports the pork quality attributes, Warner-Bratzler Shear Force, Slice Shear Force, Star Probe, pH, marbling, color (Minolta L*/L or Hunter L*/L), and sensory tenderness evaluation, in control groups used in comparative nutrition experiments over the past 20 yr. The original aim of this study was to evaluate if changes in pork quality based on the above metrics occurred over time. To address this question, it was anticipated that data may come from 3 sources with decreasing relevance: representative retail pork surveys, representative post-harvest carcass surveys, and control groups from comparative nutrition experiments. To identify the study population, a review of studies reported in Centre for Agricultural Biosciences International Abstracts (Web of Knowledge; 1994–2014) was conducted. Two national level surveys of retail pork and 146 relevant nutritional experiments studies, with 228 control groups, were identified by the search. It was not possible to conduct a meta-analysis of the retail pork surveys based on only 2 time points. For the comparative studies, a random effects meta-analysis was conducted with year as a covariate to assess the impact of time on the outcome. In the absence of modifiers, there was no evidence of meaningful change in the mean Warner-Bratzler Shear Force, pH, color, marbling, or sensory scores over the study period. There was evidence of substantial between-study heterogeneity in the characteristics of control pigs used over the years for Warner-Bratzler Shear Force and measures of color. The absence of publicly-available representative surveys of pork quality meant the changes in pork quality over time were not clear. If changes in pork quality have occurred, the data suggest that pigs used as controls in experiments may have become less representative of commercial pigs over time and the translatability of study findings from nutrition experiments might be reduced over time. Alternately, if commercial pigs have not changed, then control pigs reflect this. The study does not address if control groups in other experimental intervention studies had similar tenderness patterns as reported here for nutritional interventions. A large amount of potentially available data was excluded from the analysis due to incomplete reporting in the original study reports.
Effects of specialty proteins as alternatives to bovine or porcine spray-dried plasma in non-medicated diets fed to weaned pigs housed in an unsanitary environment
Authors: Crenshaw J. D. , Campbell J. M. , Polo J. , Stein H. H. 
Published: December 2018

AbstractTwo experiments were done to compare growth performance of pigs weaned at 21 ± 2 d of age that were housed in unsanitary pens and fed non-medicated diets containing alternative proteins versus spray-dried porcine (SDPP; Exp. 1) or bovine (SDBP; Exp. 2) plasma. Experiment 1 used 360 pigs fed 1 of 6 experimental diets from d 0 to 15, followed by a common diet fed to all pigs from d 15 to 28 post-weaning with 11 pens of 5 or 6 pigs/pen allotted per treatment. Experimental diets were based on 8.04% soy protein concentrate (SPC) as the control protein or a similar diet with either 2.50 or 5.00% SDPP or 0.17, 0.33, or 1.00% activated porcine plasma (APP) replacing SPC on an equal Lys basis. Experiment 2 used 300 pigs that were fed 1 of 6 experimental diets from d 0 to 14 post-weaning with 10 pens of 4 to 6 pigs/pen allotted per diet. Experiment 2 diets were based on 8.04% SPC as the control protein source or similar diets with the following specialty proteins replacing SPC on an equal Lys basis: 0.40% APP; 10.66% enzymatically hydrolyzed soy and yeast protein (EHSY); a combination (CB) of 6.36% EHSY, 0.40% APP, and 2.50% fish meal; 0.44% spray-dried whole egg from hyper-immunized hens (IEGG); or 5.00% SDBP. Results of Exp. 1 indicated pigs fed SDPP diets had greater (P &lt; 0.05) ADG and ADFI at d 7 and 15 compared with pigs fed SPC or APP diets. Gain:feed at d 7 was higher (P &lt; 0.05) for pigs fed diets with SDPP compared with other diets. Average BW at d 7 was greater (P &lt; 0.05) for pigs fed diets with SDPP compared to other diets and pigs fed the 5.00% SDPP diet had greater BW at d 15 compared to diets without SDPP. At d 28 BW was greater (P &lt; 0.05) for pigs fed the 2.50% SDPP diet compared with pigs fed diets with SPC, 0.33% APP, or 1.00% APP. Performance of pigs fed the SPC diet did not differ from APP diets at any period of the study. In Exp. 2, pigs fed the SDBP diet had greater (P &lt; 0.05) BW, ADG, and ADFI at d 7 and 14 compared with pigs fed the other diets. Gain:feed did not differ significantly among diets. Average daily gain and ADFI of pigs did not differ among diets that did not contain SDBP. In conclusion, during the initial 2 wk post-weaning, pigs housed in unsanitary pens and fed non-medicated diets with APP in Exp. 1, or APP, EHSY, CB, or IEGG in Exp. 2 had equivalent performance to pigs fed SPC; however, performance of pigs fed diets with the alternatives was not equivalent to diets containing SDPP in Exp. 1 or SDBP in Exp. 2.
Modeling the effects of standardized ileal digestible isoleucine to lysine ratio on growth performance of nursery pigs1,2
Authors: Clark A. B. , Tokach M. D. , DeRouchey J. M. , Dritz S. S. , Goodband R. D. , Woodworth J. C. , Touchette K. J. , Bello N. M. 
Published: December 2018

Abstract
Two experiments evaluated the effects of increasing standardized ileal digestible (SID) Ile:Lys ratio on growth performance of nursery pigs. In both experiments, dietary treatments consisted of 40, 44, 48, 52, 54, 58, or 63% SID Ile:Lys ratio. Diets were formulated using analyzed ingredient AA values and NRC (2012) SID coefficients. A combination of field peas and spray dried blood cells were used to ensure a low enough Ile diet concentration while minimizing the excess of Leu. The experiments consisted of 8 pens per dietary treatment with 5 pigs per pen for a total of 280 nursery pigs per experiment (Exp. 1: PIC 327 × 1,050, initially 6.7 ± 1.0 kg BW; Exp. 2: DNA 600 × 241, initially 6.0 ± 0.97 kg BW). Data were analyzed using mixed models with heterogeneous variance, where appropriate. The dose response was further characterized using quadratic polynomial (QP), broken-line linear (BLL), or broken-line quadratic (BLQ) functional forms. For Exp. 1, diets were initiated 6-d post-weaning and fed for 12-d followed by a common diet from d 12 to 28. From d 0 to 12, increasing dietary SID Ile:Lys ratio increased ADG (linear, P &lt; 0.005) and ADFI (quadratic, P &lt; 0.017) but G:F decreased (quadratic, P &lt; 0.043). For ADG, the QP, BLL, and BLQ models resulted in maximum ADG at 64.7, 52.0, and 52.0 SID Ile:Lys ratios, respectively. For ADFI, the BLL breakpoint occurred at 50.6 and the QP predicted maximum ADFI at 56.2 SID Ile:Lys ratio. In Exp. 2, diets were initiated 6-d post-weaning for 7 pens and 3-d post-weaning for one heavier block and fed for 18-d followed by a common diet from d 18 to 32. From d 0 to 18, ADG and ADFI increased (quadratic, P &lt; 0.016) with no evidence for difference in G:F as SID Ile:Lys ratio increased. For ADG, the QP and BLL had similar fit with breakpoints or maximums occurring at 58.3 and 51.8% SID Ile:Lys ratio, respectively. For ADFI, the BLQ breakpoint occurred at 52.0 SID Ile:Lys and the QP maximum ADFI at 57.2% SID Ile:Lys ratio. In conclusion, broken-line models reported maxima of 52.0% Ile:Lys ratio while quadratic models were as high as 64% of Lys to maximize ADG and ADFI of 6- to 11-kg nursery pigs. However, for the QP models 99% of the maximum response was achieved with a dose comparable to that from the broken line models. Therefore, these results are similar to the NRC (2012) requirement estimate of 51.1 Ile:Lys ratio.
Modeling the effects of standardized ileal digestible valine to lysine ratio on growth performance of nursery pigs1,2
Authors: Clark A. B. , Tokach M. D. , DeRouchey J. M. , Dritz S. S. , Goodband R. D. , Woodworth J. C. , Touchette K. J. , Bello N. M. 
Published: December 2018

Abstract
Two experiments evaluated the effects of increasing Lys and Val on growth performance of nursery pigs. In Exp. 1,300 nursery pigs (PIC 327 × 1,050, initially 6.7 ± 1.4 kg BW) were randomly allotted to 1 of 6 diets containing 1.10, 1.20, 1.30, 1.40, 1.50, or 1.60% standardized ileal digestible (SID) Lys, with 10 pens per dietary treatment and 5 pigs per pen. Linear and nonlinear mixed models were fitted to estimate dose responses. From d 0 to 14, and for the overall 28 d period, ADG and G:F increased (linear, P &lt; 0.001) as SID Lys increased, with no evidence of differences in ADFI. Dose response modeling indicated the SID Lys requirement for ADG and G:F was at 1.45% using a broken line linear (BLL) and greater than 1.60% using a quadratic polynomial (QP) model. In Exp. 2, 280 nursery pigs (PIC 327 × 1,050, initially 6.5 ± 1.3 kg BW) were allotted to 1 of 7 diets containing SID Val:Lys ratios of 50, 57, 63, 68, 73, 78, or 85%. The dietary SID Lys concentration 1.24% SID Lys which was below the estimated requirement from Exp. 1 and ensured the Val:Lys ratio was not underestimated. From d 0 to 14, ADG, ADFI, and G:F increased (quadratic, P &lt; 0.039) with increasing SID Val:Lys. For ADG, the best fitting model was a BLL, with a breakpoint estimate of 62.9% SID Val:Lys [52.2, 73.7] ratio while for G:F the best fit model was a quadratic polynomial with a maximum G:F at 71.7% SID Val:Lys (95%CI:[58, &gt; 85]). Average daily feed intake was also modeled with a quadratic polynomial and maximized at 73.7% Val:Lys (95% CI: [61, &gt; 85]). In conclusion, the Val requirement ranged from approximately 63 to 74% of Lys depending on the response criteria modeled.