bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Reference Genome Sequence of
Corynebacterium mastitidis
16-1433, Isolated from a Mouse
Authors: Cheleuitte-Nieves Christopher , Gulvik Christopher A. , Humrighouse Ben W. , Bell Melissa E. , Villarma Aaron , Westblade Lars F. , Lipman Neil S. , Fischetti Vincent A. , McQuiston John R. 
Published: February 2018

ABSTRACT

We report here a nearly complete draft genome sequence for a
Corynebacterium mastitidis
isolate from a mouse. The total read coverage is 198×, and the genome size is 2,264,319 bp with a 69.04% GC content. This genome complements the only other genome available for
C. mastitidis
, which was obtained from a sheep.
Complete Genome Sequence of the First Isolate of Hibiscus Latent Singapore Virus Detected in Japan
Authors: Yoshida Tetsuya , Kitazawa Yugo , Neriya Yutaro , Hosoe Naoi , Fujimoto Yuji , Hagiwara-Komoda Yuka , Maejima Kensaku , Yamaji Yasuyuki , Namba Shigetou 
Published: February 2018

ABSTRACT
The complete genome sequence of the first Japanese isolate of hibiscus latent Singapore virus (HLSV-J) was determined. The genomes of HLSV-J and a reported isolate from Singapore had only 86.7% nucleotide identity, while the encoded proteins shared amino acid identities of more than 95%.
Full-Length Genome Sequences of Two Chinese Porcine Circovirus Type 3 Strains, NWHEB21 and NWHUN2
Authors: Liu Can , Chen Shasha , Meng Fanwei , Chen Rui , Zhang Zhigang , Du Enqi , Xue Qinghong 
Published: February 2018

ABSTRACT
Two porcine circovirus 3 (PCV3) strains, named NWHEB21 and NWHUN2, were identified in heart and brain tissues of aborted piglets. Their complete genome sequences were sequenced and analyzed to further characterize PCV3 in China and worldwide.
Draft Genome Sequence of
Listeria monocytogenes
Strain CIIMS-PH-1, a Serovar 4b Isolate from Infant Septicemia
Authors: Kishnani Pooja M. , Tiwari Ankita A. , Gautam Vikas , Sharma Megha , Barbuddhe Sukhadeo B. , Doijad Swapnil P. , Chakraborty Trinad , Nayak Amit R. , Bhartiya Nidhi M. , Daginawala Hatim F. , Singh Lokendra R. , Kashyap Rajpal S. 
Published: February 2018

ABSTRACT

We report here the draft genome sequence of
Listeria monocytogenes
CIIMS-PH-1, an isolate obtained from a 16-day-old infant with septicemia. The draft genome of CIIMS-PH-1 consisted of 2,939,183 bp and is a member of sequence type 308, clonal complex 1, and lineage I.
Characterization of a Vesivirus Associated with an Outbreak of Acute Hemorrhagic Gastroenteritis in Domestic Dogs
Authors: Renshaw Randall W. , Griffing Jennifer , Weisman Jaime , Crofton Lisa M. , Laverack Melissa A. , Poston Robert P. , Duhamel Gerald E. , Dubovi Edward J. 
Published: February 2018

ABSTRACT

Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence homology to a vesivirus within the group colloquially known as the vesivirus 2117 strains that were originally identified as contaminants in CHO cell bioreactors.
In situ
hybridization and reverse transcription-PCR assays of tissues from the four deceased dogs confirmed the presence of canine vesivirus (CaVV) nucleic acids that localized to endothelial cells of arterial and capillary blood vessels. CaVV nucleic acid corresponded to areas of necrosis and hemorrhage primarily in the intestinal tract, but also in the brain of one dog with nonsuppurative meningoencephalitis. This is the first report of an atypical disease association with a putative hypervirulent vesivirus strain in dogs, as all other known strains of CaVV appear to cause nonclinical infections or relatively mild disease. After identification of the CU-296 vesivirus strain from this outbreak, four additional CaVV strains were amplified from unrelated fecal specimens and archived stocks provided by other laboratories. Broader questions include the origins, reservoir(s), and potential for reemergence and spread of these related CaVVs.
Efficacy and safety of the Shexiang Baoxin Pill for the treatment of coronary artery disease not amenable to revascularisation: study protocol for a randomised, placebo-controlled, double-blinded trial
Authors: Tian Pan-pan , Li Jun , Gao Jian , Li Ying 
Published: February 2018

IntroductionCoronary artery disease (CAD) not amenable to revascularisation indicates that the coronary arteries have severe diffuse lesions or calcifications, or that CAD is complicated with severe multiple-organ disease. Currently, Western medicines available for the treatment of CAD not amenable to revascularisation are limited. Shexiang Baoxin Pill (SBP), a type of Chinese patent medicine, has been widely used to treat CAD in China for many years. Previous studies have shown that long-term administration of SBP (1–2 pills three times daily, for at least 6 months) for treatment of CAD is effective and safe, with a significant, long-term effect. This study aims to evaluate the efficacy and safety of SBP in patients with CAD not amenable to revascularisation.Methods and analysisThis is a multicentre, randomised, double-blinded, placebo-controlled clinical trial. A total of 440 participants will be randomly allocated to two groups: the intervention group and the placebo group. Based on conventional treatment with Western medicine, the intervention group will be treated with SBP and the placebo group will be treated with SBP placebo. The primary outcomes include major adverse cardiovascular events (including angina, acute myocardial infarction, pulmonary embolism and aortic dissection). The secondary outcomes include C reactive protein, B-type natriuretic peptide, ECG, echocardiographic parameters (ejection fraction percentage and the E/A ratio) and hospital readmission rates due to CAD. Assessments will be performed at baseline (before randomisation) and at 24 weeks after randomisation.Ethics and disseminationThe protocol has been approved by the Research Ethics Committee of Guang’anmen Hospital, China Academy of Chinese Medical Sciences in Beijing, China (reference: 2016-129-KY-01). The results of this study will be published in a peer-reviewed journal and will be used as a basis for a multisite trial.Trial registration numberNCT03072121; Pre-results.
The possible link between coeliac and Kawasaki diseases in Brazil: a cross-sectional study
Authors: dos Santos Domingues Alessandra , Selleski Nicole , Uenishi Rosa Harumi , Medeiros Ribeiro de Magalhães Cristina , Gandolfi Lenora , Pratesi Claudia B 
Published: February 2018

BackgroundKawasaki disease (KD) is a self-limited acute systemic vasculitis of unknown aetiology that predominantly affects infants and young children eventually associated with immunological abnormalities. Coeliac disease (CD) is an inflammatory autoimmune disease characterised by a permanent gluten intolerance, which affects genetically susceptible individuals of any age group, and can cause intestinal and systemic symptoms. Association of CD with KD has been previously described in a single study that disclosed a surprisingly high prevalence of CD in children with a history of KD.ObjectiveTo confirm the existence of a higher prevalence of CD among individuals with a history of KD, which would turn the screening for CD in patients with history of KD highly advisable.SettingChildren with history of KD, diagnosed and followed at the Rheumatology Clinic of the Children’s Hospital of Brasilia (Brasilia, Brazil).ParticipantsThis study included 110 children with history of KD and a control group composed of 110 presumably healthy children.InterventionsParticipants underwent anti-transglutaminase and anti-endomysial antibodies tests and genetic typing for the presence of CD predisposing alleles (HLA-DQ2 and DQ8). Jejunal biopsy was performed when necessary, according the European Society of Paediatric Gastroenterology, Hepatology and Nutrition guidelines.ResultsDiagnosis of CD was confirmed in one (0.91%) patient with KD by positive serological tests, presence of predisposing alleles and CD typical lesions on duodenal biopsy. All serological tests were negative among the controls. The prevalence of CD predisposing alleles among patients with KD was 29.09%, similar to the prevalence found among controls, 33.64%.ConclusionThe detected CD prevalence (0.91%) does not confirm the existence of an association between KD and CD since this prevalence is similar to that found in the general population (≃1%).
A scoping review protocol to map the evidence on interventions to prevent overweight and obesity in children
Authors: Bussiek Peer-Benedikt Vincent , De Poli Chiara , Bevan Gwyn 
Published: February 2018

IntroductionObesity has become one of the biggest public health problems of the 21st century. Prevalence of obesity in children and adolescents has increased dramatically worldwide over the last 20 years, and this trend is expected to continue. Obesity in childhood is concerning as it predicts obesity in adulthood, a common risk factor for a wide array of chronic diseases and poor health outcomes. Obesity is preventable and a vast but fragmented body of evidence on preventative interventions is now available. This article outlines the protocol for a scoping review of published literature reviews on interventions to prevent obesity in children. The scoping review addresses the broad research question ‘What is the evidence on interventions to prevent childhood obesity?’. It aims to give an overview of the various interventions available, understand those which are effective and identify barriers and facilitators to their effectiveness.Methods and analysisThe six-staged Arksey and O’Malley methodology framework is used to guide the scoping review process: following the definition of the research questions (stage 1); the eligibility criteria and search strategy are defined (stage 2); the study selection process based on the eligibility criteria identified will follow (stage 3); a framework developed for this review will then inform the extraction and charting of data from the included reviews (stage 4); results will be aggregated and summarised with criteria relevant for health professionals and policy-makers (stage 5); and the optional consultation (stage 6) exercise is not planned.Ethics and disseminationSince the scoping review methodology aims at synthetising information from available publications, this study does not require ethical approval. An article reporting the results of the scoping review will be submitted for publication to a scientific journal, presented at relevant conferences and disseminated as part of future workshops with professionals involved in obesity prevention.
Comparison of Glasgow Admission Prediction Score and Amb Score in predicting need for inpatient care
Authors: Cameron Allan , Jones Dominic , Logan Eilidh , O’Keeffe Colin A , Mason Suzanne M , Lowe David J 
Published: February 2018

AimWe compared the abilities of two established clinical scores to predict emergency department (ED) disposition: the Glasgow Admission Prediction Score (GAPS) and the Ambulatory Score (Ambs).MethodsThe scores were compared in a prospective, multicentre cohort study. We recruited consecutive patients attending ED triage at two UK sites: Northern General Hospital in Sheffield and Glasgow Royal Infirmary, between February and May 2016. Each had a GAPS and Ambs calculated at the time of triage, with the triage nurses and treating clinicians blinded to the scores. Patients were followed up to hospital discharge. The ability of the scores to discriminate discharge from ED and from hospital at 12 and 48 hours after arrival was compared using the area under the curve (AUC) of their receiving-operator characteristics (ROC).Results1424 triage attendances were suitable for analysis during the study period, of which 567 (39.8%) were admitted. The AUC for predicting admission was significantly higher for GAPS at 0.807 (95% CI 0.785 to 0.830), compared with 0.743 (95% CI 0.717 to 0.769) for Ambs, P<0.00001. Similar results were seen when comparing ability to predict hospital stay of >12 hour and >48 hour. GAPS was also more accurate as a binary test, correctly predicting 1057 outcomes compared with 1004 for Ambs (74.2vs70.5%, P=0.012).ConclusionThe GAPS is a significantly better predictor of need for hospital admission than Ambs in an unselected ED population.
Hypoxic Challenge Testing (Fitness to Fly) in children with complex congenital heart disease
Authors: Naqvi Nitha , Doughty Victoria L , Starling Luke , Franklin Rodney C , Ward Simon , Daubeney Piers E F , Balfour-Lynn Ian M 
Published: February 2018

ObjectiveCommercial airplanes fly with an equivalent cabin fraction of inspired oxygen of 0.15, leading to reduced oxygen saturation (SpO2) in passengers. How this affects children with complex congenital heart disease (CHD) is unknown. We conducted Hypoxic Challenge Testing (HCT) to assess need for inflight supplemental oxygen.MethodsChildren aged <16 years had a standard HCT. They were grouped as (A) normal versus abnormal baseline SpO2 (≥95% vs <95%) and (B) absence versus presence of an actual/potential right-to-left (R–L) shunt. We measured SpO2, heart rate, QT interval corrected for heart rate and partial pressure of carbon dioxide measured transcutaneously (PtcCO2). A test failed when children with (1) normal baseline SpO2 desaturated to 85%, (2) baseline SpO285%–94% desaturated by 15% of baseline; and (3) baseline SpO275%–84% desaturated to 70%.ResultsThere were 68 children, mean age 3.3 years (range 10 weeks–14.5 years). Children with normal (n=36) baseline SpO2 desaturated from median 99% to 91%, P<0.0001, and 3/36 (8%) failed the test. Those with abnormal baseline SpO2 (n=32) desaturated from median 84% to 76%, P<0.0001, and 5/32 (16%) failed (no significant difference between groups). Children with no R–L shunt (n=25) desaturated from median 99% to 93%, P<0.0001, but 0/25 failed. Those with an actual/potential R–L shunt (n=43) desaturated from median 87% to 78%, P<0.0001, and 8/43 (19%) failed (difference between groups P<0.02). PtcCO2, heart rate and QT interval corrected for heart rate were unaffected by the hypoxic state.ConclusionsThis is the first evidence to help guide which children with CHD need a preflight HCT. We suggest all children with an actual or potential R–L shunt should be tested.
Systematic review of caregiver burden in spouses and partners providing informal care to wounded, injured or sick (WIS) military personnel
Authors: Thandi Gursimran , Harden L , Cole L , Greenberg N , Fear N T 
Published: February 2018

IntroductionFor the purposes of this review, caregivers are individuals who provide care that is typically unpaid and usually takes place at home. This systematic review aims to identify burden among spouses/partners caring for wounded, injured or sick military personnel and the factors associated with caregiver burden.MethodsA systematic review was undertaken using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses reporting guidelines. Five electronic databases and relevant websites were searched. Two reviewers appraised the quality of the studies and carried out data extraction.ResultsTen original papers were identified, of which eight were quantitative studies and two were qualitative. These papers highlighted the potential negative impact caregiving can have on spouses/partners and also some of the positive aspects of caring that can strengthen intimate relationships.ConclusionsCaring for an injured or ill military spouse or partner is a difficult task, compounded by the complexity of dealing with potentially both their physical and mental health problems. However, research has also identified some positive aspects of caring that can strengthen intimate relationships.
Organisational commitment as a moderating role in the relationship between power distance perception and outcome variables
Authors: Rafiei Sima , Abdollahzade Sina 
Published: February 2018

BackgroundHealthcare organisations have mainly emphasised on significant role of human resources in improving organisational performance and obtaining competitive advantage. This study aimed to investigate the relationship between perception of justice among hospital employees and outcome variables such as organisational commitment, job satisfaction and readiness to accept job responsibilities. It further examined the impact of job commitment on the relationship between perception of power distance, job satisfaction and readiness to accept responsibilities.MethodsA cross-sectional, descriptive design study was conducted among hospital employees affiliated by Qazvin University of Medical Sciences, Qazvin, Iran. A standard questionnaire taken from Francesco and Chen study was applied to gather data. Descriptive statistics, correlation coefficient, linear regression and moderated multiple regression were used to analyse study data using SPSS V.20.ResultsStudy findings showed that the level of power distance perceived by employees had a significant relationship with job satisfaction, employee participation, organisational commitment and readiness to accept job responsibilities (P<0.05). Moderated multiple regression results supported the hypothesis that job commitment had a significant moderating effect on the relationship between perception of justice, job satisfaction and readiness to work (β1=0.7, β2=0.4, P<0.05).ConclusionApplying strengthening strategies to improve employee commitment to their work and job responsibilities help organisations maintain more satisfied workforce who have positive perception towards justice in the workplace.
Detection of QTL underlying seed quality components in soybean [Glycine max (L.) Merr.]
Authors: Akond Masum , Yuan Jiazheng , Liu Shiming , Kantartzi Stella K. , Meksem Khalid , Bellaloui Nacer , Lightfoot David A. , Kassem My Abdelmajid 
Published: February 2018

Improving seed composition and quality, including protein, oil, fatty acid, and amino acid contents, is an important goal of soybean farmers and breeders. The aim of this study was to map the quantitative trait loci (QTL) underlying the contents of protein, oil, fatty acids, and amino acids with 1510 single nucleotide polymorphism (SNP) markers using the ‘Hamilton’ × ‘Spencer’ recombinant inbred line population (H × S; n = 93). A total of 13 QTL for the traits studied have been mapped on 3 chromosomes (Chr.) of the soybean genome. Three major QTL have been mapped to a 7–13 cM region on Chr. 6. One major QTL for oil content (qOIL001) explained approximately 76% of the total phenotypic variation in this population; the second major QTL for amino acid alanine (Ala; qALA001) explained approximately 74% of the total variation in Ala content; moreover, two major QTL for palmitic acid (qPAL001 and qPAL002) were identified on Chr. 6 and explained approximately 21% of the phenotypic variation in this population. The SNP markers flanking the QTL identified here will be very useful for soybean breeders to develop and select soybean lines with higher seed composition qualities using marker-assisted selection.
Tank mixture of glyphosate with 2,4-D accentuates 2,4-D injury in glyphosate-resistant corn
Authors: Soltani Nader , Shropshire Christy , Sikkema Peter H. 
Published: February 2018

Six field trials were conducted at Ridgetown, ON, over a 2-yr period (2015 and 2016) to determine the tolerance of two corn hybrids to 2,4-D (560 and 1120 g a.i. ha−1) and glyphosate (1800 g a.e. ha−1) applied alone or in combination at stage V1, V3, or V5. In DeKalb DKC52-61 corn, 2,4-D caused as much as 24%, 16%, 11%, and 11% visible injury at 1 wk after each postemergence herbicide application (WAT), 2 WAT, 4 wk after the last postemergence herbicide application (WA-C), and 8 WA-C, respectively. Plant stand was not affected, but plant height decreased by 5 cm at 560 g a.i. ha−1 and 7% at 1120 g a.i. ha−1. As the application timing was delayed from stage V1 to V5, there was a trend to increased injury at both 2,4-D rates. Corn yield decreased 8% with 2,4-D applied at 560 g a.i. ha−1 and 12% at 1120 g a.i. ha−1. In Pioneer P0094AM corn, 2,4-D caused as much as 16%, 9%, 7%, and 7% visible injury at 1 WAT, 2 WAT, 4 WA-C, and 8 WA-C, respectively. Plant height was not affected, but goosenecking and brace root malformation were increased as the rate of 2,4-D was increased. There was generally no difference between glyphosate rates (1800 vs 0 g a.e. ha−1) at the V1 corn stage but visible injury, goosenecking, and brace root malformation at other application timings was as much as 15%, 3%, and 19% greater when 2,4-D was applied in a tank mixture with glyphosate, respectively. Yield was reduced by 12% when 2,4-D (1120 g a.i. ha−1) was applied with glyphosate in the tank mixture.
Study on brain glucose metabolic networks in Parkinson’s disease patients with visual spatial dysfunction by 18F-FDG PET imaging
Authors: Tan Haibo , Li Xiuming , Wei Kai , Guan Yihui 
Published: February 2018

Aim: To assess the changes of brain glucose metabolism and abnormal intracerebral loop in early Parkinson’s disease (PD) patients with visual spatial dysfunction by [Formula: see text]F-fluorodeoxyglucose ([Formula: see text]F-FDG) positron emission tomography (PET) imaging. Materials and Methods: This study includes three groups: early PD patients with visual spatial dysfunction ([Formula: see text]), early PD patients without visual spatial dysfunction ([Formula: see text]) and healthy controls ([Formula: see text]). Resting-state [Formula: see text]F-FDG PET was performed to obtain the imaging of brain glucose metabolism. Statistical Parametric Mapping (SPM) was used for data analyses to compare the brain glucose metabolic changes among different groups. Results: Compared with the healthy controls, early PD patients (with/without visual spatial dysfunction) showed hypermetabolism in putamen, globus pallidus, thalamus, pons, cerebellum and primary motor cortex, and hypometabolism in part of the occipital and temporal lobes. Compared with early PD patients without visual spatial dysfunction, those with visual spatial dysfunction further showed hypometabolism in visual regions including bilateral lateral prefrontal cortices and posterior parietal lobules, besides occipital and temporal lobes. Conclusion: The occurrence of abnormal glucose metabolism in the brain visual processing areas was closely associated with visual spatial dysfunction in PD patients.
Body mass index interaction with SCN10A effects on cardiovascular autonomic neuropathy risk in a Chinese Han population
Authors: Wang Guo-Zhuo , Liu Zhi-Jun , Xia Kai , Zhang Ke-Qin , Tang Zi-Hui 
Published: February 2018

Background: The aim of this study was to estimate the extent to which the interaction between SCN10A (rs7375096) and body mass index (BMI) affects susceptibility to cardiovascular autonomic neuropathy (AN) in a Chinese Han sample. Method: We conducted a large-scale, population-based study including 791 overweight patients and 1186 controls to analyze the interaction between SCN10A and BMI with regard to CAN. All participants were genotyped for the presence of SCN10A (rs7375036) polymorphisms. Univariate and multiple linear regression analyses were employed to detect these relationships. The interaction between rs7375036 and BMI with regard to CAN susceptibility on an additive scale was estimated by using the relative excess risk due to interaction, the proportion attributable to interaction, and the synergy index. Results: Univariate analyses found no significant association between rs7375036 single nucleotide polymorphism and CAN. After controlling for potential confounding factors, the multiple linear regression analysis showed a significant association between BMI and CAN ([Formula: see text], OR = 1.121). In addition, a significant positive interaction effect of BMI and rs7375036 on CAN was detected ([Formula: see text]). Conclusion: Our findings suggest that BMI and SCN10A (rs7375036) have interaction effects that influence the CAN.