bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neurological involvement in patients with systemic autoimmune rheumatic diseases: a descriptive study in an Egyptian cohort
Authors: Attia Doaa Hassan Sayed , Fotouh Alshaimaa Aboul , Mohammed Reem Hamdy Abdellatif 
Published: December 2024

Abstract
Background
Neurologic manifestations in the systemic autoimmune rheumatic diseases (SARDs) are protean. They add to the disease burden and could contribute to mortality. Increasing awareness about the neuro-rheumatologic syndromes might help with early diagnosis and effective therapy. Our aim is to survey the clinical and imaging patterns of neurological involvement in Egyptian patients with SARDs.

Results
Neurological involvement is common in Behçet’s disease (BD) (12.7%) and systemic lupus erythematosus (SLE) (6.4%) patients compared with other SARDs. Compared with SLE, neurological involvement in BD tends to develop at an older age (31 ± 7.1 versus 28.3 ± 9.6 years = 0.022) with a greater progression risk (13.8% versus 2.6%, P = 0.003). A higher proportion of SLE patients had abnormal neuroimaging without neurological symptoms (15.7% versus 4.3%, P = 0.026, OR = 4.9, 95%CI 1.1–22.4). SLE patients had a higher frequency of seizures (31.3% versus 6.4%, P < 0.001, OR = 6.7, 95%CI 2.7–16.7) and benign intracranial hypertension (9.6% versus 1.1%, P = 0.009, OR = 9.8, 95%CI 1.2–77.7) but a lower prevalence of quadriplegia due to brain insult (1.7% versus 3.2%, P = 0.045, OR = 0.2, 95%CI 0.04–0.9), dural sinus thrombosis (13% versus 33%, P = 0.001, OR = 0.3, 95%CI 0.2–0.6), brainstem syndrome (0.9% versus 6.4%, P = 0.047, OR = 0.1, 95%CI 0–1.1) and cranial neuropathies (9.6% versus 31.9%, P < 0.001, OR = 0.2, 95%CI 0.1–0.5). Concerning neuroimaging, brain atrophic changes were more common (27.4% versus 9.5%, P = 0.002, OR = 3.6, 95%CI 1.6–8.3) while thrombosis was less prevalent (36.3% versus 53.6%, P = 0.016, OR 0.5, 95%CI = 0.3–0.9) in lupus patients. The cerebral cortex was more commonly affected (20.4 versus 4.8%, P = 0.002, OR = 5.1, 95%CI 1.7–15.4) while dural sinuses (14.2% versus 40.5%, P < 0.001, OR = 0.2, 95%CI 0.1–0.5), basal ganglia (1.8% versus 10.7%, P = 0.010, OR = 0.2, 95%CI 0–0.7), diencephalon (0% versus 13.1%, P < 0.001) and brainstem (1.8% versus 22.6%, P < 0.001, OR = 0.1, 95%CI 0–0.3) were less frequently involved in SLE patients. Concerning other SARDs, cranial neuropathies were the most common neurological presentations. Abnormalities in neuroimaging did not correlate with the patients’ clinical presentations.

Conclusions
Neurological presentations associated with SARDs are protean. Neuroimaging abnormalities should be interpreted within the context of the clinical picture and the results of other investigations.
Transitioning from the Green Revolution to the Gene Revolution: strengthening nutritional security using climate resilient traditional crops
Authors: Tahakik Rushikesh R. , Deshmukh Amit G. , Moharil Mangesh P. , Jadhav Pravin V. , Kogade Vinod T. , More Kalyani D. , Shinde Vijay P. 
Published: December 2024

Abstract
Background
World food production has significantly increased, largely attributed to advancements in breeding methods pioneered in previous decades. These advancements can be categorized into three major eras—the pre-Green Revolution era, the Green Revolution era, and the post-Green Revolution era—which we call the Gene Revolution. The pre-Green Revolution era was characterized by the utilization of mutation breeding and forward genetics approaches.

Main body
The Green Revolution (1950s–1980s) brought about major improvements in crop breeding, irrigation, and fertilizer use, leading to a substantial rise in food production and reduced rural poverty. However, the focus was primarily on yield, with less focus on nutritional quality. After the Green Revolution, the production of wheat and rice rapidly increased from 0.4–2 metric tonnes per hectare to 2–4 metric tonnes per hectare due to government initiatives. Nevertheless, the cultivation of other food crops, including indigenous rice varieties and millet, has decreased. In the Gene Revolution era, while advanced genome editing technologies such as CRISPR‒Cas9 and TALENs have enhanced the nutritional content and climate resilience of crops, there has also been a renewed focus on reviving indigenous crops.

Conclusion
This review discusses methods to bring these orphan nutria crops back into cultivation and emphasizes the importance of passing this knowledge to future generations, ensuring their place in sustainable agriculture and food and nutritional security.
Outcome predictors in patients with temporal lobe epilepsy after temporal resective surgery
Authors: Yu Jiabin , Li Yinchao , Xie Xuan , Cheng Liming , Zhu Shaofang , Sui Lisen , Wu Youliang , Xie Xuemin , Xie Haitao , Zhang Xiaojing , Chen Chun , Liu Yingying 
Published: December 2024

Abstract
Background
Temporal lobe epilepsy is one of the most common types of partial epilepsy. Although surgical treatment has led to significant improvements in seizure-free rates, nearly one-third of patients still have poor seizure control after surgery. Moreover, the long-term outcome is less favorable compared to short-term outcome, with 48–58% of patients experiencing seizures five years after surgery. The aim of this study was to investigate the surgical outcomes and the predictive value of prognostic factors associated with poor surgical outcomes in temporal lobe epilepsy patients receiving surgery.

Methods
We retrospectively reviewed 94 patients undergoing temporal resective surgery in the Epilepsy Center of Guangdong Provincial Hospital of Traditional Chinese Medicine between July 2016 and July 2020. Patient information including age, gender, personal and family history, as well as preoperative and postoperative clinical data (clinical type and duration of disease) was collected.

Results
The differences of postoperative clinical efficacy in both seizure free group and non-seizure free group patients were observed. A log-rank test was used for univariate analysis, and a Cox proportional hazard model was used for multivariate analysis. Ninety-four patients were followed up for at least 1 years. At 12 months of follow-up, 71 (75.5%) patients achieved Engel class I, 5 (5.3%) patients were classified as Engel class II, 5 (5.3%) patients were classified as Engel class III, and 13 (13.8%) patients were classified as Engel class IV. Univariate analysis and multivariate Cox regression analysis indicated that the postoperative EEG abnormalities were significantly correlated with seizure recurrence and were significant independent predictive factors, with a hazard ratio of 12.940.

Conclusions
The relapse rate in our study was similar to commonly reported overall rates in temporal lobe epilepsy patients receiving surgery. Anterior temporal lobectomy is a reliable treatment option for temporal lobe epilepsy patients. Postoperative electroencephalograph abnormalities are independent risk factors for poor surgical prognosis.
A rare case of Gorlin–Goltz syndrome
Authors: Hassan Sara R. , Youssef Ayda A. , ElMallah Esraa S. 
Published: December 2024

Abstract
Background
The Gorlin–Goltz syndrome (the Nevoid basal cell carcinoma syndrome (NBCCS)) is reviewed as a rare autosomal dominant syndrome caused by gene mutations located on chromosome arm 9 q. It has a variety of presentations and is characterized by basal cell carcinomas (BCC), odontogenic keratocysts (OKCs), and ectopic calcifications of the falx cerebri or plantar pits. The diagnosis depends mainly on a number of minor and major criteria. This syndrome can be misdiagnosed, which makes its early detection important in preventing consensual complications.

Case presentation
In our case a 50-year-old male was reported and presented with one minor and four major criteria of the Gorlin–Goltz syndrome, the first presentation was pathologically proven facial basosquamous carcinoma post-surgical excision, subsequent contrast-enhanced maxillofacial MRI as well as CT Head, Neck revealed OKCs of the maxillary and mandible as well as calcifications of the falx and vertebral fusion with palmar pits on physical examination with definite diagnosis of Gorlin–Goltz syndrome.

Conclusion
Gorlin–Goltz syndrome is an unusual inherited multi-systemic syndrome, which may be misdiagnosed. It is crucial that health care specialists have the necessary knowledge of the fundamental features of NBCC syndrome and the determination of its associated disorders. All patients should undergo genetic screening as well as their first-degree relatives. Morbidity can be avoided by early treatment of BCC and OKCs to avoid deformities of surrounding dermal and osseous structures.
Bioactive phytochemicals from leaf extract of Erlangea tomentosa S. Moore (Bothriocline longipes (Oliv. & Hiern.) used to treat bacterial infections in Uganda
Authors: Namukobe Jane , Ekyibetenga Yeremiah , Oyedeji Adebola Omowunmi , Byamukama Robert , Heydenrech Mathias 
Published: December 2024

Abstract
Background
The leaves of Erlangea tomentosa (ET) are used to treat several bacterial infections like respiratory tract infections, skin infections, diarrhea and gastrointestinal disorders. However, there is limited information about the compounds responsible for its bacterial activities. The study aimed at isolation and characterisation of phytochemicals from the leaf extracts of ET.

Results
Three compounds were isolated from leaf extracts of ET and characterised. Two compounds; eriodictyol-7-O-β-glucoside (1) and 5, 7, 3′, 4′-tetrahydroxy-7-O-[6"-O-(acetyl)-β-D-glucopyrancsyloxy]-flavanone (coccinoside A) (2) were identified from ethyl acetate extract while one compound; di-(2-ethylhexyl) phthalate (3) was identified from dichloromethane extract.

Conclusion
Compounds; 1, 2 and 3 were isolated and identified from ET and genus Erlangea for the first time. The reported antibacterial properties of compound 3 in literature could be responsible for the antibacterial activities of ET and the traditional use of ET.
Acetyltransferase NAT10 promotes an immunosuppressive microenvironment by modulating CD8+ T cell activity in prostate cancer
Authors: Liu Ji , Gu Zhuoran , Zou Libin , Zhang Zhijin , Shen Liliang , Wang Ruiliang , Xue Shaobo , Geng Jiang , Mao Shiyu , Zhang Wentao , Yao Xudong 
Published: December 2024

AbstractN-acetyltransferase 10 (NAT10), an enzyme responsible for ac4C acetylation, is implicated in cancer progression, though its specific biological function in prostate cancer remains insufficiently understood. This study clarifies NAT10’s role in prostate cancer and its effects on the tumor immune microenvironment. NAT10 expression and clinical relevance were assessed through bioinformatics, RT-qPCR, and IHC analyses, comparing prostate cancer tissues with normal controls. The impact of NAT10 on tumor cell proliferation, migration, and invasion was investigated via in vitro assays—including CCK-8, EdU, wound healing, and 3D-Transwell—as well as in vivo mouse xenograft models and organoid studies. Further, NAT10’s influence on immune cell infiltration was examined using flow cytometry, IHC, cell co-culture assays, and ELISA to elucidate downstream chemokine effects, specifically targeting CD8+ T cells. Findings indicated significant upregulation of NAT10 in prostate cancer cells, enhancing their proliferative and invasive capacities. Notably, NAT10 suppresses CD8+ T cell recruitment and cytotoxicity through the CCL25/CCR9 axis, fostering an immunosuppressive microenvironment that exacerbates tumor progression. An ac4C modification score was also devised based on NAT10’s downstream targets, providing a novel predictive tool for evaluating immune infiltration and forecasting immunotherapy responses in patients with prostate cancer. This study underscores NAT10’s pivotal role in modulating the prostate cancer immune microenvironment, offering insights into the immune desert phenomenon and identifying NAT10 as a promising therapeutic target for improving immunotherapy efficacy.
Diverse immune cell profiles in ASFV-associated lymphopenia
Authors: Xiong Wenjing , Chen Haowei , Chen Yanru , Wang Ke , Lian Tingting , Zhang Weijia , Yu Qing , Gao Xiaochen , Su Jie , He Qigai , Wang Xiangru , Yu Junping , Cui Min 
Published: December 2024

AbstractPathogenic African swine fever virus (ASFV) remains a lethal causative agent in the domestic pig industry, which poses a burden on the swine market and causes substantial socioeconomic losses worldwide. Currently, there are no commercially efficacious vaccines or specific treatments available for ASF prevention and control. Unfortunately, little is known about the swine immune response upon ASFV infection. Here, we investigated the host immune response discrepancy induced by the field moderately virulent strain ASFV HB-2208 among healthy, diseased and asymptomatic pigs. In the peripheral blood of diseased swine, lymphopenia is caused by the massive loss of bystander lymphocytes, such as γδ T cells, B cells and CD4+ T cells. Conversely, ASFV has a strong tropism for the mononuclear phagocyte system (MPS) and partial dendritic cells (DCs), whose antigen-presenting ability is impeded by the downregulation of CD80 and MHC I. However, no significant difference in the number of CD8αhigh T cells was detected, whereas the frequencies of NK cells, NKT cells, and regulatory T cells (Tregs) were significantly increased. Additionally, an in vitro model was established with a coculture of primary pulmonary alveolar macrophages (PAMs) and peripheral blood mononuclear cells (PBMCs), which significantly reduced γδ T cells, B cells and CD4+ T cells and increased Tregs. The differentiated immune response might aid in enhancing the understanding of ASFV pathogenesis in suids and provide insights into the mechanism of ASFV-induced lymphopenia for further studies.
Volumetric Breast Density Estimation From Three-Dimensional Reconstructed Digital Breast Tomosynthesis Images Using Deep Learning
Authors: Ahluwalia Vinayak S. , Doiphode Nehal , Mankowski Walter C. , Cohen Eric A. , Pati Sarthak , Pantalone Lauren , Bakas Spyridon , Brooks Ari , Vachon Celine M. , Conant Emily F. , Gastounioti Aimilia , Kontos Despina 
Published: December 2024

PURPOSE
Breast density is a widely established independent breast cancer risk factor. With the increasing utilization of digital breast tomosynthesis (DBT) in breast cancer screening, there is an opportunity to estimate volumetric breast density (VBD) routinely. However, current available methods extrapolate VBD from two-dimensional (2D) images acquired using DBT and/or depend on the existence of raw DBT data, which is rarely archived by clinical centers because of storage constraints.

METHODS

We retrospectively analyzed 1,080 nonactionable three-dimensional (3D) reconstructed DBT screening examinations acquired between 2011 and 2016. Reference tissue segmentations were generated using previously validated software that uses 3D reconstructed slices and raw 2D DBT data. We developed a deep learning (DL) model that segments dense and fatty breast tissue from background. We then applied this model to estimate %VBD and absolute dense volume (ADV) in cm
3
in a separate case-control sample (180 cases and 654 controls). We created two conditional logistic regression models, relating each model-derived density measurement to likelihood of contralateral breast cancer diagnosis, adjusted for age, BMI, family history, and menopausal status.

RESULTS

The DL model achieved unweighted and weighted Dice scores of 0.88 (standard deviation [SD] = 0.08) and 0.76 (SD = 0.15), respectively, on the held-out test set, demonstrating good agreement between the model and 3D reference segmentations. There was a significant association between the odds of breast cancer diagnosis and model-derived VBD (odds ratio [OR], 1.41 [95 % CI, 1.13 to 1.77];
P
= .002), with an AUC of 0.65 (95% CI, 0.60 to 0.69). ADV was also significantly associated with breast cancer diagnosis (OR, 1.45 [95% CI, 1.22 to 1.73];
P
< .001) with an AUC of 0.67 (95% CI, 0.62 to 0.71).

CONCLUSION
DL-derived density measures derived from 3D reconstructed DBT images are associated with breast cancer diagnosis.
An Infant With DHX37 Variant: A Novel Etiology of 46,XY DSD and Literature Review
Authors: Turk Yilmaz R Sena , Hittelman Adam B , Vash-Margita Alla , Dinauer Catherine , Weinzimer Stuart A , Gujral Jasmine 
Published: December 2024

Abstract
46,XY sex reversal 11 (SRXY11) is a rare and recently identified form of 46,XY difference in sexual development (DSD), caused by variants in the DEAH-Box Helicase 37 gene (DHX37). DHX37 is crucial for ribosome biogenesis, but its specific role in gonadal development remains unclear. The genital phenotype varies widely, ranging from typical female to typical male. We present a 46,XY infant with prenatal ultrasound findings of atypical genitalia. Amniotic fluid gene analysis revealed a known heterozygous pathogenic variant in DHX37, p.R308Q (c.923G&gt;A), confirmed postnatally. The patient was born with markedly undervirilized genitalia with posteriorly fused labioscrotal folds, a single introitus, no clitoromegaly, and nonpalpable gonads. Laboratory evaluation at multiple points showed undetectable anti-Müllerian hormone (AMH) and inhibin B levels, elevated gonadotropin levels, and negligible testosterone levels. Clinical course was complicated by urine retention in the vagina and uterus and hydronephrosis requiring catheterization. Endoscopy revealed a urogenital sinus with separate urethral and vaginal openings and 2 cervices leading into 2 separate uteri suggestive of a bicornuate bicollis uterus. Laparoscopy revealed 2 intra-abdominal gonads adjacent to the fallopian tubes. Evidence for inheritance, penetrance, genotype-phenotype correlation, and risk of malignancy in SRXY11 is limited to case reports.
Altered Ca2+ responses and antioxidant properties in Friedreich's ataxia-like cerebellar astrocytes
Authors: Marullo Chiara , Croci Laura , Giupponi Iris , Rivoletti Claudia , Zuffetti Sofia , Bettegazzi Barbara , Cremona Ottavio , Giunti Paola , Ambrosi Alessandro , Casoni Filippo , Consalez Gian Giacomo , Codazzi Franca 
Published: December 2024

ABSTRACT
Friedreich's ataxia (FRDA) is a neurodegenerative disorder characterized by severe neurological signs, affecting the peripheral and central nervous system, caused by reduced frataxin protein (FXN) levels. Although several studies have highlighted cellular dysfunctions in neurons, there is limited information on the effects of FXN depletion in astrocytes and on the potential non-cell autonomous mechanisms affecting neurons in FRDA. In this study, we generated a model of FRDA cerebellar astrocytes to unveil phenotypic alterations that might contribute to cerebellar atrophy. We treated primary cerebellar astrocytes with an RNA interference-based approach, to achieve a reduction of FXN comparable to that observed in individuals with FRDA. These FRDA-like astrocytes display some typical features of the disease, such as an increase of oxidative stress and a depletion of glutathione content. Moreover, FRDA-like astrocytes exhibit decreased Ca2+ responses to purinergic stimuli. Our findings shed light on cellular changes caused by FXN downregulation in cerebellar astrocytes, likely impairing their complex interaction with neurons. The potentially impaired ability to provide neuronal cells with glutathione or to release neuromodulators in a Ca2+-dependent manner could affect neuronal function, contributing to neurodegeneration.
A Qualitative Study on the Experiences of Patients with Pulmonary Hypertension Undergoing Polypharmacy
Authors: Wang Yundan , Huang Run , Zhuang Yiyu 
Published: December 2024

Aims/Background Pulmonary hypertension (PH) is a severe progressive disease characterized by elevated blood pressure in the lungs. Medications are a critical form of treatment for patients with PH. This study aims to explore the experiences of patients with PH undergoing polypharmacy, thereby providing a more concrete foundation for formulating targeted intervention measures. Methods A purposive sampling method was used to select 13 PH patients treated in a grade III hospital in Zhejiang Province from December 2023 to February 2024 as the research subjects. This study is a descriptive qualitative research design, where the patients were interviewed face-to-face in a semi-structured manner. The data were analyzed, summarized and extracted using the traditional content analysis method. Results There were five themes and ten sub-themes surrounding the polypharmacy experiences of the PH patients: (1) Negative emotional experience of multidrug use, including lack of disease-related medication knowledge and insufficient ability to obtain medication information; (2) Complex physical testing of multiple drugs, including adverse reactions and complex dose titration; (3) Economic burden; (4) Coping difficulties, including insufficient drug safety, excessive reliance on medical personnel, and lack of opportunities for deep participation; (5) Desire for supports, including professional and social support, as well as those from family, colleagues and classmates. Conclusion Disease, treatment, psychological factors, and social customs affect PH patients’ drug use post-diagnosis, with varying experiences and degrees. Healthcare providers must offer tailored care and practical strategies for managing multiple drug use, considering patients’ unique experiences and needs.
Effectiveness of a BOPPPS Teaching Model in Standardized Training for Nephrology Resident Physicians: A Retrospective Cohort Study
Authors: Li Yuting , Ren Fei , Qiu Jin , Li Defu , Wang Jiangmei , Lin Shan 
Published: December 2024

Aims/Background Based on Bridge-in, Objective, Pre-assessment, Participatory learning, Post-assessment and Summary (BOPPPS), the teaching model has gained increasing attention in the field of medical education. This study aimed to evaluate the effectiveness of the BOPPPS teaching model in standardized training for nephrology residents, particularly in educating on hyperkalemia in chronic kidney disease (CKD). Methods This retrospective cohort study included students undergoing standardized training in the nephrology department at the Shanghai Traditional Chinese Medicine-Integrated Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from 2021 to 2024. The observation group (n = 55) received instructions using the BOPPPS teaching model, while the control group (n = 64) was educated using the traditional teaching methods. The study evaluated learning outcomes and teaching satisfaction through theoretical and practical assessments, as well as self-assessment by the students. Results The observation group demonstrated significantly higher scores in theoretical exams and practical assessments compared to the control group (p < 0.05). Additionally, the observation group reported higher self-assessment scores (p < 0.05) and greater teaching satisfaction (p < 0.05) than the control group. Conclusion The BOPPPS teaching model is an effective approach to enhancing theoretical knowledge, practical skills, and teaching satisfaction in standardized training for nephrology residents. Compared to traditional teaching methods, the BOPPPS model improves learning outcomes, thereby strengthening the quality of medical education.
Vorgehen bei der Einführung einer Organisation für das Management von Enterprise Systems
Authors: Krause Sven , Jung Jürgen 
Published: December 2024

Zusammenfassung
Der vorliegende Beitrag beschreibt die Einführung einer Organisationeinheit für das Enterprise Architecture Management in einem deutschen Logistikunternehmen. Die Anwendungslandschaft besteht aus wenigen Eigenentwicklungen sowie verschiedenen Standardsoftwaresystemen, von denen einige durch Konfiguration, andere aber durch Nicht-Standard-Erweiterungen angepasst werden. Die Einführung der Organisationseinheit erfolgt im Rahmen eines Action-Research-Projects, welches sich über drei Phasen gezogen hat. Ziel des Beitrags ist die Ableitung von Empfehlungen für ähnliche Vorhaben auf Basis der Erprobung von Konzepten aus der Literatur sowie den Erfahrungen aus ihrer praktischen Umsetzung.