bluuebunny/crossref_metadata_2025_split huggingface dataset:
SPHK1‐induced autophagy in peritoneal mesothelial cell enhances gastric cancer peritoneal dissemination
Authors: Yin Songcheng , Miao Zhifeng , Tan Yuen , Wang Pengliang , Xu Xiaoyu , Zhang Chao , Hou Wenbin , Huang Jinyu , Xu Huimian 
Published: February 2019

AbstractGastric cancer peritoneal dissemination (GCPD) has been recognized as the most common form of metastasis in advanced gastric cancer (GC), and the survival is pessimistic. The injury of mesothelial cells plays an important role in GCPD. However, its molecular mechanism is not entirely clear. Here, we focused on the sphingosine kinase 1 (SPHK1) in human peritoneal mesothelial cells (HPMCs) which regulates HPMCs autophagy in GCPD progression. Initially, we analyzed SPHK1 expression immunohistochemically in 120 GC peritoneal tissues, and found high SPHK1 expression to be significantly associated with LC3B expression and peritoneal recurrence, leading to poor prognosis. Using a coculture system, we observed that GC cells promoted HPMCs autophagy and this process was inhibited by blocking TGF‐β1 secreted from GC cells. Autophagic HPMCs induced adhesion and invasion of GC cells. We also confirmed that knockdown of SPHK1 expression in HPMCs inhibited TGF‐β1‐induced autophagy. In addition, SPHK1‐driven autophagy of HPMCs accelerated GC cells occurrence of GCPD in vitro and in vivo. Moreover, we explored the relationship between autophagy and fibrosis in HPMCs, observing that overexpression of SPHK1 induced HPMCs fibrosis, while the inhibition of autophagy weakened HPMCs fibrosis. Taken together, our results provided new insights for understanding the mechanisms of GCPD and established SPHK1 as a novel target for GCPD.
Family‐Based Recovery: An Innovative Collaboration between Community Mental Health Agencies and Child Protective Services to Treat Families Impacted by Parental Substance Use
Authors: Hanson Karen E. , Duryea Elizabeth R. , Painter Mary , Vanderploeg Jeffrey J. , Saul Dale H. 
Published: February 2019

To meet the needs of families who have very young children and are involved with child protective services due to substance use, the State of Connecticut Department of Children and Families, Yale Child Study Center and Johns Hopkins University created an innovative treatment model. This public–private collaboration required a paradigm shift for both child protective services staff and treatment providers. This brief description of the Family‐Based Recovery model highlights the family‐focused practice elements that allow children to remain safely at home with parents who are in treatment. Outcomes suggest that Family‐Based Recovery is a promising practice, and collaborations between child protective services and substance use treatment providers can yield positive results for families with young children.‘Highlights the family‐focused practice elements that allow children to remain safely at home with parents who are in treatment’Key Practitioner Messages

Strong collaboration between child protective services and treatment providers can mitigate the risk of out‐of‐home placement for children impacted by parental substance use.
In‐home treatment that provides concurrent psychotherapy, substance use treatment and parent–child dyadic therapy is an important part of the service array for families involved with child protective services.
Temporal trends of survival and utilization of mechanical circulatory support devices in patients with in‐hospital cardiac arrest secondary to ventricular tachycardia/ventricular fibrillation
Authors: Patel Nileshkumar J. , Atti Varunsiri , Kumar Varun , Panakos Andrew , Anantha Narayanan Mahesh , Bhardwaj Bhaskar , Arora Shilpkumar , Deshmukh Abhishek J , Patel Nish , Basir Mir B. , Cohen Mauricio G. , Kini Annapoorna S. , Sharma Samin K. , Dangas George , O'Neill William W. , Alfonso Carlos E. 
Published: February 2019

AbstractBackgroundPulseless ventricular tachycardia/ventricular fibrillation (VT/VF) is the initial rhythm in a third of in‐hospital cardiac arrest patients. Mechanical circulatory support (MCS) device use remains poorly understood in this population.MethodsWe conducted an observational analysis of temporal trends in the utilization of MCS in VT/VF IHCA between January 2008 and December 2014 utilizing the Nationwide Inpatient Sample (NIS) database. Using multivariable analysis, we assessed factors associated with MCS use and survival to discharge.ResultsAmong 151,628 hospitalizations with VT/VF IHCA, 14,981 (9.9%) received MCS. Intra‐aortic balloon pump (IABP) was the most commonly used MCS (9.1%). From 2008 to 2014, there was significant increase in the utilization of MCS (8.7–11%; ptrend < 0.0001). On multivariable analysis, there was 12‐fold increase and three‐fold increase in the utilization of PVAD and ECMO respectively; however, there was no significant change in the use of IABP. Over the seven‐year sample period, there was significant increase in the overall survival to hospital discharge (35.4–43.5%; ptrend < 0.0001). Survival to hospital discharge increased in both MCS and non‐MCS groups.ConclusionThere was significant increase in utilization of MCS after VT/VF IHCA during the study period. IABP was the most commonly utilized MCS. The survival to hospital discharge increased in the overall study population including both MCS and non‐MCS groups. Future studies are needed to identify patient population most likely to benefit from the use of MCS after VT/VF IHCA.
Outcomes in patients with peripheral vascular disease following percutaneous coronary intervention
Authors: Ramzy John , Andrianopoulos Nick , Roberts Louise , Duffy Stephen J. , Clark David , Teh Andrew W. , Ajani Andrew E. , Reid Christopher M. , Brennan Angela , Freeman Melanie ,   On behalf of the Melbourne Interventional Group (MIG)
Published: February 2019

AbstractObjectivesTo evaluate the clinical characteristics and outcomes of patients with peripheral vascular disease (PVD) undergoing percutaneous coronary intervention (PCI) in a contemporary setting, and to determine whether use of drug‐eluting stents (DESs) improves outcomes.BackgroundPVD was an independent risk factor for adverse outcomes following PCI in the bare‐metal stent (BMS) era. It is not known whether outcomes in these patients have improved with advances in interventional techniques and stent technology, as they have for the general population.MethodsEighteen thousand three hundred and eighty patients undergoing PCI from an Australian registry between 2005 and 2013 were studied. Clinical and procedural data, 30‐day and 12‐month outcomes were compared in those with and without a reported history of PVD. Outcomes were also compared between patients with PVD who received DES and those who received BMS. Long‐term mortality was compared using Australian National Death Index (NDI) linkage.ResultsPatients with PVD (n = 1,251, 6.8%) were older and had more prevalent diabetes, hypertension, cerebrovascular disease, heart failure, renal impairment, ostial lesions, left main, and multi‐vessel disease (p < 0.001). Patients with PVD had significantly higher rates of major adverse cardiovascular events (MACEs) compared with those without PVD, in‐hospital (5.7% vs. 4.1%, p < 0.008), at 30‐days (8.6% vs. 5.8%, p < 0.001) and at 12‐months (24.6% vs. 13.2%, p < 0.001). At 4.9 ± 2.6 years follow‐up, there was significantly greater mortality in the PVD group. PVD patients who received DES experienced significantly less MACE than PVD patients treated with BMS at 30‐days (4.8 vs. 10.1%, p < 0.001) and 12‐months (19.4 vs. 26.4%, p < 0.005).ConclusionsPVD is an independent predictor of adverse outcomes in patients undergoing PCI. PVD patient who received DES had improved outcomes compared with those receiving BMS.
Photoisomerization of PtII Complexes Containing Two Different Photochromic Chromophores: Boron Chromophore versus Dithienylethene Chromophore
Authors: Li Xue , Shi Yonggang , Wang Nan , Peng Tai , Wang Suning 
Published: February 2019

AbstractIn order to examine competitive photoisomerization, a series of novel photochromic PtII molecules that contain both dithienylethene (DTE) and B(ppy)Mes2 units (ppy=2‐phenylpyridine, Mes=mesityl) were successfully synthesized and fully structurally characterized. Their photochromic properties were examined by UV/Vis, emission and NMR spectroscopy. It was found that the DTE unit in all three compounds is the preferred photoisomerization site, exhibiting reversible photochromism with irradiation. The B(ppy)Mes2 unit does not undergo photoisomerization in these molecules, but likely enhances the photoisomerization quantum efficiency of the DTE moiety through the antenna effect. Extended irradiation with UV light leads to the rearrangement of the ring‐closed isomers of DTE. TD‐DFT computational studies indicate that the DTE photocyclization proceeds via a triplet pathway through an efficient energy transfer process.
Metal‐Free Electrochemical Reduction of Carbon Dioxide Mediated by Cyclic(Alkyl)(Amino) Carbenes
Authors: Lieske Lauren E. , Freeman Lucas A. , Wang Guocang , Dickie Diane A. , Gilliard Robert J. , Machan Charles W. 
Published: February 2019

AbstractCarbenes are known to activate carbon dioxide to form zwitterionic adducts. Their inherent metal‐free redox activity remains understudied. Herein, we demonstrate that zwitterionic adducts of carbon dioxide formed with cyclic(alkyl)(amino) carbenes are not only redox active, but they can mediate the stoichiometric reductive disproportionation of carbon dioxide to carbon monoxide and carbonate. Infrared spectroelectrochemical experiments show that the reaction proceeds through an intermediate radical anion formed by one‐electron reduction, ultimately generating a ketene product and carbonate in the absence of additional organic or inorganic reagents.
Anti‐Mi‐2 antibody titers and cutaneous manifestations in dermatomyositis
Authors: Ogawa‐Momohara Mariko , Muro Yoshinao , Akiyama Masashi 
Published: February 2019

AbstractThe anti‐Mi‐2 antibody is a dermatomyositis (DM)‐specific autoantibody. It is thought to be associated with photosensitivity in patients with DM because higher expression levels of Mi‐2 antigens have been found in UV‐irradiated cultured cells and because epidemiological studies have supported this association. This study aims to identify the relations between anti‐Mi‐2 antibody titers measured by ELISA and clinical characteristics in patients with DM. We focused on analyzing associations between anti‐Mi‐2 antibody titers and cutaneous manifestations. This retrospective analysis covered 160 adult DM patients. Anti‐Mi‐2 antibodies were detected by an in‐house ELISA, and anti‐Mi‐2 titers were measured by a manufactured ELISA. Twelve of the 160 (7.5%) patients were anti‐Mi‐2 positive. The anti‐Mi‐2‐positive patients showed extremely elevated muscle enzyme levels (P < 0.0001), less frequent interstitial lung disease complication (P = 0.031), and favorable survival rate (100%) (P = 0.07). No correlations were found between anti‐Mi‐2 antibody titers and clinical characteristics or skin manifestations, including photosensitive rashes. Further investigation is necessary to elucidate the pathological significance of the anti‐Mi‐2 antibody in DM.
Efficient spectrum sensing framework for cognitive networks
Authors: Jacob T. Prem , Pravin A. , Nagarajan G. 
Published: February 2019

SummaryCognitive network is a modern networking technology that utilizes both the radio spectrum and the wireless station. The resources can be effectively utilized based on availability of knowledge gathered from the past experiences. By using cognitive radio technology, the new network is constructed to every node. Wireless devices' necessity is being increased nowadays, which leads to the demand for spectrum. The problems are high fluctuations of the different spectrum bands and the disconnections of the network. The cognitive radio network is proposed to be an optimal solution for both the spectrum scarcity and the spectrum inefficiency problems. Using the cognitive radio network, the wireless channel is shared with the primary users. The cognitive radio network provides high bandwidth for the mobile users by using specialized heterogeneous wireless architecture. These challenges are overcome by CR networks that define the concept of spectrum sensing. While using the spectrum, the performance of the network should not degrade. Hence, the framework planned will able to do this situation.
Detection and Quantification of Multiple RNA Sequences Using Emerging Ultrasensitive Fluorescent In Situ Hybridization Techniques
Authors: Erben Larissa , Buonanno Andres 
Published: February 2019

AbstractFluorescent detection of transcripts using RNAscope has quickly become a standard in situ hybridization (ISH) approach in neuroscience with over 400 publications since its introduction in 2012. RNAscope's sensitivity and specificity allow the simultaneously detection of up to three low abundance mRNAs in single cells (i.e., multiplexing) and, in contrast to other ISH techniques, RNAscope is performed in 1 day. BaseScope, a newer ultrasensitive platform, uses improved amplification chemistry of single oligonucleotide probe pairs (∼50 bases). This technique allows discrimination of single nucleotide polymorphisms or splice variants that differ by short exons. A present limitation of BaseScope is that expression analysis is limited to a single gene (i.e., single‐plexing). This article outlines detailed protocols for both RNAscope and BaseScope in neuronal tissue. We discuss how to perform ISH experiments using either fresh‐frozen or formalin‐fixed paraffin‐embedded sections, as well as dissociated cultured neurons. We also outline how to obtain quantitative data from hybridized tissue sections. © 2019 by John Wiley & Sons, Inc.
Efficient Bioconversion of Sucrose to High‐Value‐Added Glucaric Acid by In Vitro Metabolic Engineering
Authors: Su Hui‐Hui , Guo Ze‐Wang , Wu Xiao‐Ling , Xu Pei , Li Ning , Zong Min‐Hua , Lou Wen‐Yong 
Published: February 2019

AbstractGlucaric acid (GA) is a major value‐added chemicals feedstock and additive, especially in the food, cosmetics, and pharmaceutical industries. The increasing demand for GA is driving the search for a more efficient and less costly production pathway. In this study, a new in vitro multi‐enzyme cascade system was developed, which converts sucrose efficiently to GA in a single vessel. The in vitro system, which does not require adenosine triphosphate (ATP) or nicotinamide adenine dinucleotide (NAD+) supplementation, contains seven enzymes. All enzymes were chosen from the BRENDA and NCBI databases and were expressed efficiently in Escherichia coli BL21(DE3). All seven enzymes were combined in an in vitro cascade system, and the reaction conditions were optimized. Under the optimized conditions, the in vitro seven‐enzyme cascade system converted 50 mm sucrose to 34.8 mm GA with high efficiency (75 % of the theoretical yield). This system represents an alternative pathway for more efficient and less costly production of GA, which could be adapted for the synthesis of other value‐added chemicals.
Stilbene derivatives as new perspective in antifungal medicinal chemistry
Authors: De Filippis Barbara , Ammazzalorso Alessandra , Amoroso Rosa , Giampietro Letizia 
Published: February 2019

AbstractThe high incidence and mortality of invasive fungal infections and serious drug resistance have become a global public health issue. There is an urgent need for alternative antimicrobials to control fungal infections and targeting it by antifungal substances from the natural sources represents a promising new strategy for the development of novel antifungal agents. Resveratrol (3,5,4′‐trihydroxy‐trans‐stilbene) is a phytoalexin produced by plant species in response to environmental stress or pathogenic attacks. It has many known and potential therapeutic applications in human general homeostasis; it mediates a great number of biological responses relevant for human health such as anticancer, cardio and neuroprotective, antioxidant, and antimicrobial activities. Resveratrol is a natural antifungal agent, therefore it can be considered as a scaffold for designing structural relatives potentially capable of mediating more intense responses in a more specific way. Also, stilbenes produced by several plants may be useful lead structure for the chemical synthesis of antifungal. Their antifungal potential represents a useful solution to the drug resistance and side effect complications that occur after pharmacological treatment of infectious diseases. The purpose of this review is to present an overview on resveratrol derivatives, both natural and synthetic, with antifungal activity and summarize the chemical structure and the therapeutic versatility of stilbene‐containing compounds.
Shared and unique features of bacterial communities in native forest and vineyard phyllosphere
Authors: Miura Toshiko , Sánchez Roland , Castañeda Luis E. , Godoy Karina , Barbosa Olga 
Published: February 2019

AbstractPhyllosphere bacteria have received little attention despite their important roles in shaping plant performance traits. In this study, we characterize the bacterial communities on leaves of native trees inhabiting sclerophyllous forests in central Chile, one of the world's biodiversity hotspots. Additionally, we provide profiles of bacterial communities on grape leaves and berries of organic and conventional vineyards. Results of 16S rRNA gene amplicon sequence analysis showed that 45% of OTUs were shared across forest leaves, grape leaves, and grape berries. Conventional management had higher number of OTUs shared with forest leaves than organic management. In addition, grape leaves subjected to conventional management had higher alpha diversity than those with organic management, while no significant effect of agricultural management was observed in grape berries. Indicator analysis showed that Bdellovibrio, Beijerinckia, and Spirosoma were typical for forest leaves, whereas Enhydrobacter, Delftia, Proteiniclasticum, Arsenicicoccus, and Alkaliphilus were typical for the vineyard phyllosphere. Regarding agricultural managements, Beijerinckia, Sedimentibacter, Nesterenkonia, Gluconobacter, Conexibacter, and Anaeromyxobacter were typical for conventional grape leaves, whereas no genus‐level indicator was found for organic vineyard leaves. These results provide new insights of the diversity patterns of the phyllosphere microbiome in native and cultivated lands and suggest that both of these microbiomes are connected and integrated systems.
NT‐proBNP is a weak indicator of cardiac function and haemodynamic response to exercise in chronic heart failure
Authors: Parovic Milos , Okwose Nduka C. , Bailey Kristian , Velicki Lazar , Fras Zlatko , Seferovic Petar M. , MacGowan Guy A. , Jakovljevic Djordje G. 
Published: February 2019

AbstractAimsN‐terminal prohormone of brain natriuretic peptide (NT‐proBNP) plays an important role in diagnosis and management of heart failure. The aim of the present study was to assess haemodynamic response to exercise and to evaluate the relationship between NT‐proBNP, cardiac function, and exercise tolerance in chronic heart failure.Methods and resultsA single‐centre, cross‐sectional pilot study recruited 17 patients with chronic heart failure with reduced left ventricular ejection fraction (age 67 ± 7 years) and 20 healthy volunteers (age 65 ± 12 years). The NT‐proBNP was measured in the heart failure group. All participants completed maximal graded cardiopulmonary exercise stress testing coupled with gas exchange (using metabolic analyser for determination of exercise tolerance, i.e. peak O2 consumption) and continuous haemodynamic measurements (i.e. cardiac output and cardiac power output) using non‐invasive bioreactance technology. Heart failure patients demonstrated significantly lower peak exercise cardiac function and exercise tolerance than healthy controls, i.e. cardiac power output (5.0 ± 2.0 vs. 3.2 ± 1.2 W, P < 0.01), cardiac output (18.2 ± 6.3 vs. 13.5 ± 4.0 L/min, P < 0.01), heart rate (148 ± 23.7 vs. 111 ± 20.9 beats/min, P < 0.01), and oxygen consumption (24.3 ± 9.5 vs. 16.8 ± 3.8 mL/kg/min, P < 0.01). There was no significant relationship between NT‐proBNP and cardiac function at rest, i.e. cardiac power output (r = −0.28, P = 0.28), cardiac output (r = −0.18, P = 0.50), and oxygen consumption (r = −0.18, P = 0.50), or peak exercise, i.e. cardiac power output (r = 0.18, P = 0.49), cardiac output (r = 0.13, P = 0.63), and oxygen consumption (r = −0.05, P = 0.84).ConclusionsLack of a significant and strong relationship between the NT‐proBNP and measures of cardiac function and exercise tolerance may suggest that natriuretic peptides should be considered with caution in interpretation of the severity of cardiac dysfunction and functional capacity in chronic heart failure.
Rationale and design of the European multicentre study on Stem Cell therapy in IschEmic Non‐treatable Cardiac diseasE (SCIENCE)
Authors: Paitazoglou Christina , Bergmann Martin W. , Vrtovec Bojan , Chamuleau Steven A.J. , van Klarenbosch Bas , Wojakowski Wojtek , Michalewska‐Włudarczyk Aleksandra , Gyöngyösi Mariann , Ekblond Annette , Haack‐Sørensen Mandana , Jaquet Kai , Vrangbæk Karsten , Kastrup Jens ,   on behalf of the SCIENCE Investigators
Published: February 2019

AbstractAimsIschaemic heart failure (IHF) patients have a poor prognosis even with current guideline‐derived therapy. Intramyocardial injections of autologous or allogeneic mesenchymal stromal cells might improve cardiac function leading to better clinical outcome.MethodsThe SCIENCE (Stem Cell therapy in IschEmic Non‐treatable Cardiac diseasE) consortium has initiated a Horizon 2020 funded multicentre phase II study in six European countries. It is a double‐blind, placebo‐controlled trial testing the safety and efficacy of allogeneic Cardiology Stem Cell Centre Adipose‐derived Stromal Cells (CSCC_ASC) from healthy donors or placebo in 138 symptomatic IHF patients. Main inclusion criteria are New York Heart Association class II–III, left ventricular ejection fraction < 45% and N‐terminal pro‐B‐type natriuretic peptide levels > 300 pg/mL. Patients are randomized in a 2:1 pattern to receive intramyocardial injections of either CSCC_ASC or placebo. CSCC_ASC and placebo treatments are prepared centralized at Rigshospitalet in 5 mL vials as an off‐the‐shelf product. Vials are distributed to all clinical partners and stored in nitrogen vapour tanks ready to be used directly after thawing. A total of 100 × 106 CSCC_ASC or placebo are injected directly into viable myocardium in the infarct border zone using the NOGA XP system (BDS, Cordis, Johnson & Johnson, USA). Primary endpoint is a centralized core‐laboratory assessed change in left ventricular end‐systolic volume at 6‐month follow‐up measured by echocardiography. The trial started in January 2017, 58 patients were included and treated until July 2018.ConclusionThe SCIENCE trial will provide clinical data on efficacy and safety of intramyocardial cell therapy of allogeneic adipose‐derived stromal cells from healthy donors in patients with IHF.