bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of ageing and osteoarthritis on the mechanical properties of cartilage and bone in the human knee joint
Authors: Peters Abby E. , Akhtar Riaz , Comerford Eithne J. , Bates Karl T. 
Published: April 2018

AbstractOsteoarthritis is traditionally associated with cartilage degeneration although is now widely accepted as a whole-joint disease affecting the entire osteochondral unit; however site-specific cartilage and bone material properties during healthy ageing and disease are absent limiting our understanding. Cadaveric specimens (n = 12; 31–88 years) with grades 0–4 osteoarthritis, were dissected and spatially correlated cartilage, subchondral and trabecular bone samples (n = 8 per cadaver) were harvested from femoral and tibial localities. Nanoindentation was utilised to obtain cartilage shear modulus (G′) and bone elastic modulus (E). Cartilage G′ is strongly correlated to age (p = 0.003) and osteoarthritis grade (p = 0.007). Subchondral bone E is moderately correlated to age (p = 0.072) and strongly correlated to osteoarthritis grade (p = 0.013). Trabecular bone E showed no correlation to age (p = 0.372) or osteoarthritis grade (p = 0.778). Changes to cartilage G′ was significantly correlated to changes in subchondral bone E (p = 0.007). Results showed preferential medial osteoarthritis development and moderate correlations between cartilage G′ and sample location (p = 0.083). Also demonstrated for the first time was significant correlations between site-matched cartilage and subchondral bone material property changes during progressive ageing and osteoarthritis, supporting the role of bone in disease initiation and progression. This clinically relevant data indicates a causative link with osteoarthritis and medial habitual loading.
High-resolution computed tomographic analysis of tooth replacement pattern of the basal neoceratopsian Liaoceratops yanzigouensis informs ceratopsian dental evolution
Authors: He Yiming , Makovicky Peter J , Xu Xing , You Hailu 
Published: April 2018

AbstractThe dental morphology and tooth replacement pattern of Liaoceratops yanzigouensis, the earliest known neoceratopsian, are important for our understanding of the evolution of the ceratopsian dental system. Here we describe the dental morphology and tooth replacement of Liaoceratops yanzigouensis based on high-resolution computed tomographic (CT) scan data of three specimens including the holotype, the first study for basal ceratopsian. The three-dimensional reconstructions reveal some important new information, including: three teeth in the premaxilla in one side, two more teeth in the dentary than in the maxilla, incipiently developed mesial grooves on some crowns, two generations of replacement teeth within some tooth families; and most functional teeth were under heavy resorption by the replacement process, but still remained functional. Comparisons of tooth pair positions from opposite sides in the four jaw quadrants of three specimens revealed a degree of bilateral symmetry in replacement pattern. Reconstruction of Zahnreihen yields an avergae z-spacing of 2.58 with simultaneous front-to-back tooth replacement. Our study presents the earliest evidence of derived neoceratopsian traits of the complex dental batteries in ceratopsids. Most significantly, our models reveal the tracts of partially resorbed functional teeth which appears to track the growth of the jaws, traits previously undocumented in Ceratopsia.
Spinal cord gray matter segmentation using deep dilated convolutions
Authors: Perone Christian S. , Calabrese Evan , Cohen-Adad Julien 
Published: April 2018

AbstractGray matter (GM) tissue changes have been associated with a wide range of neurological disorders and were recently found relevant as a biomarker for disability in amyotrophic lateral sclerosis. The ability to automatically segment the GM is, therefore, an important task for modern studies of the spinal cord. In this work, we devise a modern, simple and end-to-end fully-automated human spinal cord gray matter segmentation method using Deep Learning, that works both on in vivo and ex vivo MRI acquisitions. We evaluate our method against six independently developed methods on a GM segmentation challenge. We report state-of-the-art results in 8 out of 10 evaluation metrics as well as major network parameter reduction when compared to the traditional medical imaging architectures such as U-Nets.
Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis
Authors: Paerl Ryan W. , Bertrand Erin M. , Rowland Elden , Schatt Phillippe , Mehiri Mohamed , Niehaus Thomas D. , Hanson Andrew D. , Riemann Lasse , Bouget Francois-Yves 
Published: April 2018

AbstractAlmost all cells require thiamin, vitamin B1 (B1), which is synthesized via the coupling of thiazole and pyrimidine precursors. Here we demonstrate that 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET) is a useful in vivo B1 precursor for representatives of ubiquitous marine picoeukaryotic phytoplankton and Escherichia coli – drawing attention to cHET as a valuable exogenous micronutrient for microorganisms with ecological, industrial, and biomedical value. Comparative utilization experiments with the terrestrial plant Arabidopsis thaliana revealed that it can also use exogenous cHET, but notably, picoeukaryotic marine phytoplankton and E. coli were adapted to grow on low (picomolar) concentrations of exogenous cHET. Our results call for the modification of the conventional B1 biosynthesis model to incorporate cHET as a key precursor for B1 biosynthesis in two domains of life, and for consideration of cHET as a microbial micronutrient currency modulating marine primary productivity and community interactions in human gut-hosted microbiomes.
Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis
Authors: Hamasaki Shinichi , Kobori Takuro , Yamazaki Yui , Kitaura Atsuhiro , Niwa Atsuko , Nishinaka Takashi , Nishibori Masahiro , Mori Shuji , Nakao Shinichi , Takahashi Hideo 
Published: April 2018

AbstractAdvanced glycation end-products (AGEs), which comprise non-enzymatically glycosylated proteins, lipids, and nucleic acid amino groups, play an important role in several diseases and aging processes including angiopathy, renal failure, diabetic complications, and neurodegenerative diseases. Among AGE-associated phenotypes, toxic AGEs, glyceraldehyde-derived AGE-2, and glycolaldehyde-derived AGE-3 are involved in the pathogenesis of diabetic complications. In addition, macrophages are reported to remove extracellular AGEs from tissues via scavenger receptors, leading to the progression of atherosclerosis. In the present study, we found that AGE-2 and AGE-3 enhanced their own endocytic uptake by RAW264.7 mouse macrophage-like cells in a concentration-dependent manner. Furthermore, we demonstrated, for the first time, the morphology of phagocytic macrophages and the endocytosis of AGE particles. The toxic AGEs induced the expression of a scavenger receptor, CD204/scavenger receptors-1 class A (SR-A). Notably, an antibody against CD204 significantly prevented toxic AGE uptake. Moreover, an SR-A antagonistic ligand, fucoidan, also attenuated the AGE-2- and AGE-3-evoked uptake in a concentration-dependent manner. These results indicated that SR-A stimulation, at least in part, plays a role in AGE uptake.
Charge mobility retrieval approach from apparent charge packet movements based on the negative differential resistance theory
Authors: Meng Jia , Zhang Yewen , Holé Stéphane , Zheng Feihu , An Zhenlian 
Published: April 2018

AbstractSpace charge migration characteristics play an important role in the evaluation of polymer insulation performance. However, an accurate description of charge carrier mobility in several typical insulating polymers such as polyethylene, polypropylene is currently not available. Recently, with the observation of a series of negative charge packet movements associated with the negative differential resistance characteristic of charge mobility in LDPE films, the extraction of charge mobility from the apparent charge packet movement has been attempted using appropriate methods. Based on the previous report of the successful derivation of charge mobility from experimental results using numerical methods, the present research improves the derivation accuracy and describes the details of the charge mobility derivation procedure. Back simulation results under several typical polarizing fields using the derived charge mobility are exhibited. The results indicate that both the NDR theory and the simulation models for the polyethylene materials are reasonable. A significant migration velocity difference between the charge carrier and the charge packet is observed. Back simulations of the charge packet under several typical polarizing fields using the obtained E-v curve show good agreement with the experimental results. The charge packet shapes during the migrations were also found to vary with the polarizing field.
Endogenous reference RNAs for microRNA quantitation in formalin-fixed, paraffin-embedded lymph node tissue
Authors: Inada Katsushige , Okoshi Yasushi , Cho-Isoda Yukiko , Ishiguro Shingo , Suzuki Hisashi , Oki Akinori , Tamaki Yoshio , Shimazui Toru , Saito Hitoaki , Hori Mitsuo , Iijima Tatsuo , Kojima Hiroshi 
Published: April 2018

AbstractLymph node metastasis is one of the most important factors for tumor dissemination. Quantifying microRNA (miRNA) expression using real-time PCR in formalin-fixed, paraffin-embedded (FFPE) lymph node can provide valuable information regarding the biological research for cancer metastasis. However, a universal endogenous reference gene has not been identified in FFPE lymph node. This study aimed to identify suitable endogenous reference genes for miRNA expression analysis in FFPE lymph node. FFPE lymph nodes were obtained from 41 metastatic cancer and from 16 non-cancerous tissues. We selected 10 miRNAs as endogenous reference gene candidates using the global mean method. The stability of candidate genes was assessed by the following four statistical tools: BestKeeper, geNorm, NormFinder, and the comparative ΔCt method. miR-103a was the most stable gene among candidate genes. However, the use of a single miR-103a was not recommended because its stability value exceeded the reference value. Thus, we combined stable genes and investigated the stability and the effect of gene normalization. The combination of miR-24, miR-103a, and let-7a was identified as one of the most stable sets of endogenous reference genes for normalization in FFPE lymph node. This study may provide a basis for miRNA expression analysis in FFPE lymph node tissue.
Response to climate change of montane herbaceous plants in the genus Rhodiola predicted by ecological niche modelling
Authors: You Jianling , Qin Xiaoping , Ranjitkar Sailesh , Lougheed Stephen C. , Wang Mingcheng , Zhou Wen , Ouyang Dongxin , Zhou Yin , Xu Jianchu , Zhang Wenju , Wang Yuguo , Yang Ji , Song Zhiping 
Published: April 2018

AbstractClimate change profoundly influences species distributions. These effects are evident in poleward latitudinal range shifts for many taxa, and upward altitudinal range shifts for alpine species, that resulted from increased annual global temperatures since the Last Glacial Maximum (LGM, ca. 22,000 BP). For the latter, the ultimate consequence of upward shifts may be extinction as species in the highest alpine ecosystems can migrate no further, a phenomenon often characterized as “nowhere to go”. To predict responses to climate change of the alpine plants on the Qinghai-Tibetan Plateau (QTP), we used ecological niche modelling (ENM) to estimate the range shifts of 14 Rhodiola species, beginning with the Last Interglacial (ca. 120,000–140,000 BP) through to 2050. Distributions of Rhodiola species appear to be shaped by temperature-related variables. The southeastern QTP, and especially the Hengduan Mountains, were the origin and center of distribution for Rhodiola, and also served as refugia during the LGM. Under future climate scenario in 2050, Rhodiola species might have to migrate upward and northward, but many species would expand their ranges contra the prediction of the “nowhere to go” hypothesis, caused by the appearance of additional potential habitat concomitant with the reduction of permafrost with climate warming.
Degradation Kinetics of Inverted Perovskite Solar Cells
Authors: Alsari Mejd , Pearson Andrew J. , Wang Jacob Tse-Wei , Wang Zhiping , Montisci Augusto , Greenham Neil C. , Snaith Henry J. , Lilliu Samuele , Friend Richard H. 
Published: April 2018

AbstractWe explore the degradation behaviour under continuous illumination and direct oxygen exposure of inverted unencapsulated formamidinium(FA)0.83Cs0.17Pb(I0.8Br0.2)3, CH3NH3PbI3, and CH3NH3PbI3−xClx perovskite solar cells. We continuously test the devices in-situ and in-operando with current-voltage sweeps, transient photocurrent, and transient photovoltage measurements, and find that degradation in the CH3NH3PbI3−xClx solar cells due to oxygen exposure occurs over shorter timescales than FA0.83Cs0.17Pb(I0.8Br0.2)3 mixed-cation devices. We attribute these oxygen-induced losses in the power conversion efficiencies to the formation of electron traps within the perovskite photoactive layer. Our results highlight that the formamidinium-caesium mixed-cation perovskites are much less sensitive to oxygen-induced degradation than the methylammonium-based perovskite cells, and that further improvements in perovskite solar cell stability should focus on the mitigation of trap generation during ageing.
Mediators of the Effects of Gender on Uric Acid Nephrolithiasis: A Novel Application of Structural Equation Modeling
Authors: Chen Hao-Wei , Chen Yu-Chen , Yang Frances M. , Wu Wen-Jeng , Li Ching-Chia , Chang Yong-Yuan , Chou Yii-Her 
Published: April 2018

AbstractNumerous epidemiological studies have shown that male patients with uric acid nephrolithiasis outnumber female patients. To our knowledge, no research exists evaluating the reasons gender affects the development of uric acid nephrolithiasis. We hereby used a novel application of structural equation modeling to analyze the mediators of the effects of gender on uric acid nephrolithiasis. In 1,098 patients with nephrolithiasis between 2012 and 2016, male gender was found to have a statistically significant positive indirect effect on the development of uric acid nephrolithiasis, which was mediated by lower urine pH (estimate: 0.010, standard error: 0.005, critical ratio: 2.135, 95% confidence interval: 0.002–0.023, P = 0.017), lower estimated glomerular filtration rate (estimate: 0.014, standard error: 0.005, critical ratio: 2.993, 95% confidence interval: 0.006–0.025, P < 0.001), and higher incidence rate of gout (estimate: 0.009, standard error: 0.005, critical ratio: 2.028, 95% confidence interval: 0.002–0.021, P = 0.009). We conclude that low urine pH, impaired renal function, and gout are the mediators of the effect of male gender on the development of uric acid nephrolithiasis. The survey, treatment, and follow-up of kidney diseases, acidic urine, and uric acid metabolism disorders should be considered in men with uric acid nephrolithiasis.
Psoriasis patients demonstrate HLA-Cw*06:02 allele dosage-dependent T cell proliferation when treated with hair follicle-derived keratin 17 protein
Authors: Yunusbaeva Milyausha , Valiev Ruslan , Bilalov Fanil , Sultanova Zilya , Sharipova Leyla , Yunusbayev Bayazit 
Published: April 2018

AbstractIt is broadly accepted that psoriasis is an immune-mediated disease with a heritable component, but it is not clear what causes inflammation in the skin. Previous research suggests that fragments of the keratin 17 (K17) protein, which are constitutively expressed in hair follicles, could act as autoantigens. In this study, we synthesized the K17 protein from mRNA derived from hair follicles and tested whether it elicited T cell responses depending on the patient genotype at the major susceptibility locus HLA-Cw*06:02. We treated peripheral blood-derived cells with the K17 protein and its short fragments to assess the T cell proliferation response using flow cytometry. Our analyses show a significantly stronger increase in cell proliferation among patients but not in healthy controls. We then examined whether the variation in T cell proliferation correlated with the patient HLA-Cw*06:02 risk genotype. Considering the affected status and patient genotype as two independent predictors, we fitted a linear model and showed that the HLA-Cw*06:02 allele dosage strongly predicted the T cell response. Our study findings suggest that the K17 protein likely acts as an autoantigen in psoriasis and that patients’ risk genotype is strongly correlated with the magnitude of the response to this putative autoantigen.
On the use of Parylene C polymer as substrate for peripheral nerve electrodes
Authors: de la Oliva Natàlia , Mueller Matthias , Stieglitz Thomas , Navarro Xavier , del Valle Jaume 
Published: April 2018

AbstractParylene C is a highly flexible polymer used in several biomedical implants. Since previous studies have reported valuable biocompatible and manufacturing characteristics for brain and intraneural implants, we tested its suitability as a substrate for peripheral nerve electrodes. We evaluated 1-year-agedin vitrosamples, where no chemical differences were observed and only a slight deviation on Young’s modulus was found. The foreign body reaction (FBR) to longitudinal Parylene C devices implanted in the rat sciatic nerve for 8 months was characterized. After 2 weeks, a capsule was formed around the device, which continued increasing up to 16 and 32 weeks. Histological analyses revealed two cell types implicated in the FBR: macrophages, in contact with the device, and fibroblasts, localized in the outermost zone after 8 weeks. Molecular analysis of implanted nerves comparing Parylene C and polyimide devices revealed a peak of inflammatory cytokines after 1 day of implant, returning to low levels thereafter. Only an increase of CCL2 and CCL3 was found at chronic time-points for both materials. Although no molecular differences in the FBR to both polymers were found, the thick tissue capsule formed around Parylene C puts some concern on its use as a scaffold for intraneural electrodes.
Characterisation and structure analysis of taxanes‐loaded human serum albumin nanoparticles prepared by self‐assembly method
Authors: Fang Ru , Wang Yanbin , Liu Bentong , Qian Hua , Yang Shaozong 
Published: April 2018

In this work, human serum albumin (HSA) was used to prepare core‐shell taxanes‐loaded nanoparticle (NP) by self‐assembly method. Various experimental technologies were used to character two different taxanes‐loaded HSA NPs and analyse the structure of them. The obtained NPs had a spherical morphology and core‐shell structure. The data revealed that one paclitaxel (PTX)‐loaded HSA NP (∼40 nm) consisted of six HSA molecules and 34 PTX molecules, and one docetaxel (DOC)‐loaded HSA NP (∼30 nm) consisted of five HSA molecules and 40 DOC molecules. The infrared, three‐dimensional fluorescence and ultraviolet absorption spectra showed that intermolecular hydrogen bond occurred between taxanes and polypeptide chain of HSA and resulted in change of its secondary structure. The fluorescence spectra experiment results suggested that PTX and DOC interacted with tryptophan (Trp)‐214, which lie on site I of HSA, and the type of interactions were hydrogen bond and hydrophobic interaction, respectively. It suggested that the binding mode of taxanes with Trp‐214 is the primary factor that determined the size and loading capacity of taxanes‐loaded HSA NPs. This work indicates that such HSA NP is very promising in the field of nanoscale drug delivery systems research.
Vein of Galen Aneurysmal Malformation: An Updated Review
Authors: Cao Li-Rong , Cai Chun-Quan 
Published: April 2018

AbstractVein of Galen aneurysmal malformation (VGAM) is a rare intracranial vascular anomaly in the choroidal arteries characterized by the persistence of the embryonic median prosencephalic vein of Markowski (MProsV). It is common in children with high mortality and disability rates. VGAM-associated clinical presentations include heart failure, hydrocephalus, and neurological symptoms, which make it difficult for diagnosis. Simultaneously, the complex VGAM vascular architecture, characterized by a wide interindividual variability, makes the management of this condition challenging to clinicians. Thus, timely diagnosis and treatment of VGAM are crucial. In this review article, we elucidated the latest research progresses of VGAM in the aspects of the pathogenesis, classification, clinical features, imaging, management, and prognosis based on recent relevant literatures. It is beneficial to raise the awareness of VGAM and improve the level of management and treatment.
Phosphorus recovery: a need for an integrated approach
Authors: Sarvajayakesavalu Suriyanarayanan , Lu Yonglong , Withers Paul. J. A. , Pavinato Paulo Sergio , Pan Gang , Chareonsudjai Pisit 
Published: April 2018

ABSTRACT

Introduction:
Phosphorus (P) is an essential element in food production. P consumption is increasing over the years due to increasing population and increasing demand for agricultural yields. Managing the agricultural P through the understanding of bioavailability, transport, and runoff will maximize the soil productivity and minimize the environmental effects. Efficient management in agriculture, governance, and lack of integrated international governance need to be addressed to overcome the P scarcity issue.

Results and Discussions:
This article is focusing one such efficient management of P resource addressing the major portion of phosphorus which is unnoticed in agricultural residues, manures, and other sources. Increasing cost of phosphate fertilizer, a scarcity of high-quality phosphate rock (PR), and increasing surface water pollution are driving a need to accelerate the recovery and reuse of phosphorus (P) from various waste sectors. Options to recover P occur all along the open P cycle from mining to households to oceans. However, P recovery as a regional and global strategy toward P sustainability and future food, bio energy, and water security are in its infancy because of a number of technological, socioeconomic, and institutional constraints. Resolving these constraints requires concerted collaboration between relevant stakeholders and an integrated approach combining successful business models with socioeconomic and institutional changes.
Wave propagation problem for a micropolar elastic waveguide
Authors: Kovalev V A , Murashkin E V , Radayev Y N 
Published: April 2018

Abstract
A propagation problem for coupled harmonic waves of translational displacements and microrotations along the axis of a long cylindrical waveguide is discussed at present study. Microrotations modeling is carried out within the linear micropolar elasticity frameworks. The mathematical model of the linear (or even nonlinear) micropolar elasticity is also expanded to a field theory model by variational least action integral and the least action principle. The governing coupled vector differential equations of the linear micropolar elasticity are given. The translational displacements and microrotations in the harmonic coupled wave are decomposed into potential and vortex parts. Calibrating equations providing simplification of the equations for the wave potentials are proposed. The coupled differential equations are then reduced to uncoupled ones and finally to the Helmholtz wave equations. The wave equations solutions for the translational and microrotational waves potentials are obtained for a high-frequency range.