bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix
Authors: Zielinski Marcin S. , Vardar Elif , Vythilingam Ganesh , Engelhardt Eva-Maria , Hubbell Jeffrey A. , Frey Peter , Larsson Hans M. 
Published: February 2019

AbstractBy analyzing isolated collagen gel samples, we demonstrated in situ detection of spectrally deconvoluted auto-cathodoluminescence signatures of specific molecular content with precise spatial localization over a maximum field of view of 300 µm. Correlation of the secondary electron and the hyperspectral images proved ~40 nm resolution in the optical channel, obtained due to a short carrier diffusion length, suppressed by fibril dimensions and poor electrical conductivity specific to their organic composition. By correlating spectrally analyzed auto-cathodoluminescence with mass spectroscopy data, we differentiated spectral signatures of two extracellular matrices, namely human fibrin complex and rat tail collagen isolate, and uncovered differences in protein distributions of isolated extracellular matrix networks of heterogeneous populations. Furthermore, we demonstrated that cathodoluminescence can monitor the progress of a human cell-mediated remodeling process, where human collagenous matrix was deposited within a rat collagenous matrix. The revealed change of the heterogeneous biological composition was confirmed by mass spectroscopy.
Interaction between GDF5 gene polymorphisms and environment factors increased the risk of knee osteoarthritis: a case–control study
Authors: Zhang Sujie , Wang Juan , Ji Hongliang , Jia Helei , Guan Dongsheng 
Published: February 2019

Abstract
Using a case–control design, we assessed the association between single nucleotide polymorphisms (SNPs) of growth and differentiation factor 5 (GDF5)/rs143383 gene and interaction with environments and knee osteoarthritis (KOA). We recruited 288 KOA patients from the First Clinical College, Henan University of Chinese Medicine between June 2017 and May 2018. There was significant difference in genotype distribution between case group and control group (χ2 = 22.661, P=0.000). The minor C allele was significantly higher in the case group than that in the control group (20.5 vs 8.1%, P=0.000, odds ratio (OR) = 1.62, 95% confidence interval (CI): 1.29–2.03). Significant differences were also observed in other gene models. For age, all models show significant differences (P&lt;0.05) for those whose age was more than 60 years, and no significant difference was observed for those under 60 years. For non-smoking group, there were significant differences between case group and control group, and for smoker, significance level was found in TT compared with CC and allele gene models. Patients with drinking and Bbody mass index (MI )≥ 24 also showed significant relationship between rs143383 and osteoarthritis (OA) under the following models: TT vs CC (P=0.000, P=0.018), TT/CT vs CC (P=0.043), TT vs CT/CC (P=0.000, P=0.009), and T vs C (P=0.024, P=0.000). Other gene models indicated no significance (P&gt;0.05). Our results revealed a possible genetic association between GDF5 and KOA, and the TT genotype of rs143383 increased the risk of KOA in Chinese Han population. The interaction between GDF5 gene and drinking, smoking, and obesity further increased the risk of KOA.
Behavioral state-dependent lateralization of dorsal dentate gyrus c-Fos expression in mice
Authors: Jordan Jake T. , Shanley M. Regis , Pytte Carolyn L. 
Published: February 2019

Abstract
Hemispheric lateralization is a fundamental organizing principle of nervous systems across taxonomic groups with bilateral symmetry. The mammalian hippocampus is lateralized anatomically, physiologically, and chemically; however, functional asymmetries are not yet well understood. Imaging studies in humans have implicated the left and right hippocampus in specialized processing. However, it is not clear if lateralized activity occurs in the rodent hippocampus. c-Fos imaging in animals provides a measure of neuronal activity with a resolution at the level of single cells. The aim of the present study was to determine whether lateralized activity-dependent c-Fos expression occurs in the rodent hippocampus. To understand functional lateralization of hippocampal processing, we compared interhemispheric expression of c-Fos in the dentate gyrus (DG), a structure involved in encoding new experiences, in mice that ran on a wheel, encoded a novel object, or remained in home cages. We found that wheel running (WR) induced the greatest amount of DG c-Fos expression in both hemispheres, with no difference between hemispheres. Object exploration (OB) resulted in left-lateralized DG c-Fos expression, whereas control (CON) mice were not lateralized. We then sought to determine whether differential consideration of hemispheres might influence the conclusions of a study by simulating common cell quantitation methods. We found that different approaches led to different conclusions. These data demonstrate lateralization of neuronal activity in the mouse DG corresponding to the experience of the animal and show that differentially considering hemisphere leads to alternative conclusions.
Trois chercheurs, dont un Français, récompensés pour leurs travaux sur les lasers
Authors: Mordon Serge R. 
Published: February 2019

Six ans après le physicien Serge Haroche, un autre Français, qui détient aussi la nationalité américaine, reçoit le prix Nobel de physique. Gérard Mourou, né en 1944 à Albertville (France), est professeur émérite à l’École polytechnique (Palaiseau, France) et ancien professeur à l’université du Michigan (Ann Arbor, États-Unis). Il partage la moitié du prix Nobel avec Donna Strickland, de l’université de Waterloo (Ontario, Canada). Née en 1959 à Guelph, au Canada, elle était alors étudiante à l’époque de ces travaux portant sur les lasers. Elle est la troisième femme seulement à être récompensée en physique, après Marie Curie en 1903 et Maria Goeppert-Mayer en 1963. L’autre moitié de cette récompense prestigieuse revient à l’Américain Arthur Ashkin, 96 ans, de l’université Cornell (Ithaca, État de New York, États-Unis). Arthur Ashkin est désormais la personne la plus âgée à voir été lauréat d’un prix Nobel, toutes catégories confondues. Ces trois chercheurs ont un point commun : ils manipulent la lumière et leurs recherches ont abouti à de nouvelles utilisations des lasers en médecine.
Avant-propos
Authors: Jouault Thierry 
Published: February 2019

Pour éclairer la série que nous vous proposons, il nous a semblé important de rappeler les règles définissant ce que sont les organismes-modèles ainsi que la réglementation en vigueur quant à leur utilisation en recherche biomédicale. Les études in vitro ou ex vivo, sur cellules ou tissus, ou les analyses in silico ne permettent pas d’aborder des problématiques complexes. Elles sont certes utiles pour des analyses mécanistiques, mais ne permettent pas l’étude de réponses biologiques intégrées qui nécessitent l’analyse d’un organisme entier. Ces organismes sont soumis à une réglementation stricte. Elle les distingue selon qu’ils sont vertébrés ou invertébrés. Si les mammifères modélisent nombre de pathologies humaines, certains organismes simples permettent également de mener ce type d’études et de répondre à des questions liées à la génétique. Leur intérêt est leur rapidité et leur capacité de reproduction qui facilitent les expérimentations. Le poisson zèbre en est un bon exemple avec les nombreuses versions de morphants qui ont été développés et qui ont permis de révéler les conséquences d’altérations génomiques sur un phénotype particulier. Cet organisme-modèle est donc particulièrement utilisé pour l’étude de pathologies monogéniques ou pour déterminer le rôle d’un gène dans l’étiologie de diverses pathologies. La transparence du poisson zèbre rend de plus les investigations plus faciles. Caenorhabditis elegans est également un excellent modèle d’organisme simple et qui, contrairement au poisson zèbre, ne relève pas de la réglementation concernant l’expérimentation animale. Son utilisation est ainsi, comme pour les insectes, plus aisée.
Connaître les différences entre les organismes-modèles, leurs avantages et leurs limites, et les règles dictant leur utilisation est donc primordial pour la réalisation d’expérimentations qui restent nécessaires à l’acquisition de nouvelles connaissances dans le domaine de la biologie et de la santé.
Körperliche Aktivität und deren Effekte auf das Burn-out-Syndrom – Ein systematisches Review
Authors: Dreher Matthias , Dößereck Nils , Lachtermann Ella 
Published: February 2019

ZusammenfassungDas vorliegende Review legt dar, dass regelmäßige körperliche Aktivität einen positiven Einfluss auf die Burn-out-Symptomatik hat. Mittels einer systematischen Literaturrecherche wurden die möglichen Effekte untersucht und die Evidenz mithilfe des Standard Index of Convergence (SIC) bewertet. Der SIC lag in den Längsschnittstudien bei SIC (3)=− 1, während er in den Interventionsstudien bei SIC (6)=− 0,50 lag. Daher ist jeweils von einer moderat starken Evidenz auszugehen, dass körperliche Aktivität einen positiven gesundheitlichen Effekt auf das Burn-out-Syndrom hat. Aufgrund der Heterogenität der Erhebungstools und der Studienlage ist es notwendig, weitere qualitativ hochwertige Studien zur Burn-out-Thematik durchzuführen, um repräsentative Aussagen zu gewährleisten. Unabhängig von den positiven präventiven und therapeutischen Effekten ist die regelmäßige körperliche Aktivität ein grundlegender, jedoch kein alleiniger Ansatzpunkt, um der Erschöpfungssymptomatik vorzubeugen und entgegenzuwirken. Eine ausgewogene Life-Balance (privates Umfeld, berufliches Umfeld und Hygiene der Grundbedürfnisse) ist daher unerlässlich.
Prevention of Cervical Cancer
Authors: Hillemanns Peter , Friese Klaus , Dannecker Christian , Klug Stefanie , Seifert Ulrike , Iftner Thomas , Hädicke Juliane , Löning Thomas , Horn Lars , Schmidt Dietmar , Ikenberg Hans , Steiner Manfred , Freitag Ulrich , Siebert Uwe , Sroczynski Gaby , Sauerbrei Willi , Beckmann Matthias , Gebhardt Marion , Friedrich Michael , Münstedt Karsten , Schneider Achim , Kaufmann Andreas , Petry K. , Schäfer Axel , Pawlita Michael , Weis Joachim , Mehnert Anja , Fehr Mathias , Grimm Christoph , Reich Olaf , Arbyn Marc , Kleijnen Jos , Wesselmann Simone , Nothacker Monika , Follmann Markus , Langer Thomas , Jentschke Matthias 
Published: February 2019

Abstract
Aims Annual opportunistic screening for cervical carcinoma has been done in Germany since 1971. The creation of this S3 guideline meets an important need, outlined in the National Cancer Plan, with regard to screening for cervical cancer, as this guideline aims to provide important information and support for planned organized screening for cervical cancer in Germany.
Methods With the financial support of German Cancer Aid, 21 professional societies developed evidence-based statements and recommendations (classified using the GRADE system) for the screening, management and treatment of precancerous conditions of the cervix. Two independent scientific institutes compiled systematic reviews for this guideline.
Recommendations The second part of this short summary deals with the triage, treatment and follow-up care of cervical dysplasia. With regard to those women who do not participate in screening, the guideline authors recommend sending out repeat invitation letters or an HPV self-collection kit. Colposcopy should be carried out for further investigation if cytology findings are Pap II-p and HPV test results are positive or if the results of an HPV 16 or HPV 18 screening test are positive. A single abnormal Pap smear should be triaged and investigated using HPV testing or p16/Ki67 dual staining.
Kosteneffektivität – Welche Auswirkungen haben Wartelisten bei
Operationen?
Authors: Thiem Helena 
Published: February 2019

Ribera A et al. The impact of waiting for intervention on costs and
effectiveness: the case of transcatheter aortic valve replacement. Eur J Health
Econ 2018; 19: 945–956
Hohe Kosten innovativer Operations- und Behandlungsmethoden können trotz eines
Zusatznutzens den Zugang für die Patienten erschweren. Eine zeitliche
Verzögerung kann dabei negative Auswirkungen auf die Mortalität und
Lebensqualität der Betroffenen haben. Aufschluss über mögliche klinische wie
ökonomische Folgen von Beschränkungen des Zugangs zu wirksamen Innovationen
soll eine Analyse der Kosteneffektivität am Beispiel von Patienten mit
Aortenklappenstenose geben.
ATP- and ADP-Induced Rat Platelet Aggregation: Significance of Plasma in ATP-Induced Aggregation
Authors: Haskel Ethan J , Agarwal Kailash C , Parks Robert E 
Published: February 2019

SummaryATP caused platelet aggregation in rat platelet-rich plasma (PRP) but in contrast strongly inhibited ADP-induced human platelet aggregation. ADP-induced aggregation of rat platelets suspended in human plasma was strongly inhibited by ATP, whereas human platelets in rat plasma were aggregated by ADP. The ATP analog β,γ-methylene ATP which is not dephosphorylated did not induce aggregation in rat PRP. Adenosine, AMP, 2- chloroadenosine, α,β-methylene ADP and β,γ-methylene ATP each inhibited ATP-induced aggregation of platelets in rat PRP to a similar extent as ADP-induced aggregation. A solution containing creatine kinase and creatine phosphate (which converts ADP to ATP) rapidly reversed both ADP- and ATP-induced aggregation in rat PRP; preincubation with this solution completely inhibited rat platelet aggregation induced by both ADP and ATP. Adenosine-8-14C-triphosphate ([14C]-ATP) conversion to [14C]-ADP was about five-fold faster in rat plasma than in human plasma. Addition of creatine phosphate to rat PRP strongly inhibited ATP-induced aggregation, while creatine or creatine kinase slightly potentiated aggregation by ATP. Creatine phosphate, creatine, or creatine kinase individually had minimal and varying effects on ADP-induced rat platelet aggregation. These results suggest that the observed phenomenon of ATP-induced aggregation in rat PRP is caused by a higher activity of creatine kinase in rat plasma than in human plasma, which converts the added ATP to ADP, a potent aggregator.
Relative Activities of Heparin Fractions Obtained by Gel Chromatography
Authors: Graham David T , Pomeroy Alan R 
Published: February 2019

SummaryIn order to investigate the reported heterogeneity of commercial heparin injections, lung and mucous preparations were fractionated by gel chromatography according to molecular size. Eluent pools were characterized by measuring electrophoretic mobility, anticoagulant activity using a reactivation assay and an anti-Xa assay, lipolytic activity and the protamine neutralization value. The only biological activity to show a marked relationship with the molecular size of the heparin fraction was the anti-Xa activation activity. This effect was more pronounced with the mucous heparins than with the lung heparin preparation studied.
Microsurgical Resection of Cerebellopontine Angle Meningioma
Authors: Cândido Duarte N. C. , Passos Gustavo A. R. , Rassi Marcio S. , de Oliveira Jean Gonçalves , Borba Luis A. B. 
Published: February 2019

Meningiomas of the cerebellopontine angle (CPA) are the second most frequent lesions related to this region (around 10–15%),1 being the vestibular schwannomas the first (around 85%). This lesions arise from the dura of the petrosal surface of the temporal bone, lateral to the trigeminal nerve (Fig. 1). Variable attachment sites and directions of growth make different clinical presentations and operative challenges. This pathologies can be classified accordingly to they're extension related to the internal acoustic meatus in: postmeatal, premeatal, and large meningiomas with pre- and postmeatal extension (Fig. 2). We present an operative video performed by the senior author (L.A.B.B.). A 64-year-old woman with 3 months of complaint of left facial pain on the V2 territory of the trigeminal nerve and diplopia secondary to VI nerve paresis. Magnetic resonance imaging (MRI) scans demonstrated a large homogeneous enhancing lesion at the left CPA, extending pre- and postmeatal and from the tentorium cerebeli to the jugular foramen region, highly suggestive of CPA meningioma. Surgery was offered to the patient as a first option. In our point of view, neurophysiological monitoring with somatosensory and motor evoked potentials is mandatory while dealing with such large tumors around the CPA. The surgery was performed after a standard retrosigmoid craniotomy, with careful dissection and debulking while devascularizing the tumor from its petrosal attachment. Near-total resection was achieved and the patient had a remarkable postoperative outcome with improvement of the diplopia and facial pain with preservation of VII and VIII nerves function. The pathology demonstrated a grade 1 meningioma.The link to the video can be found at: https://youtu.be/UVVyEhq8Fu0.
The Ultimate Skull Base Maneuver Does Not Involve Removing Bone: Quantifying the Benefits of the Interfascial Dissection
Authors: Effendi Sabih T. , Momin Eric N. , Basma Jaafar , Michael L Madison , Duckworth Edward A.M. 
Published: February 2019

Introduction Several adjunctive osteal skull base maneuvers have been proposed to increase surgical exposure of the anterolateral approach. However, one of the easiest methods does not involve bone: the interfascial temporalis muscle dissection.
Methods Sequential dissections were performed bilaterally on five fixed silicone-injected cadaver heads. The amount of sphenoid drilling, scalp retraction, and brain retraction was standardized in all specimens. For each approach, surgical angles were measured for four deep targets: the tip of the anterior clinoid process, the internal carotid artery terminus, the origin of the posterior communicating artery, and the anterior communicating artery. Five surgical angles were measured for each target.
Results There were increases on the order of 20% in the anteroposterior (AP)-mid, AP–lateral, and mediolateral–anterior angles for all deep targets with interfascial approach versus a myocutaneous flap. An orbitozygomatic osteotomy additionally increased almost all the angles, but incrementally less so.
Conclusion An interfascial dissection increases the surgical exposure to a larger degree than additional osteotomies for several surgically relevant working angles. The addition of an orbitozygomatic osteotomy affords a particular benefit for the suprachiasmatic region. Increased adoption of interfascial mobilization or the temporalis muscle—an easily performed and low-risk maneuver—during anterolateral craniotomies may obviate the need for more involved skull base drilling.
Approaching the Sella through the Nonpneumatized Sphenoid in Pediatric Patients
Authors: Oviedo Parisa , Levy Michael L. , Nation Javan 
Published: February 2019

Objective The purpose of this study is to specifically assess pediatric patients with nonpneumatized sphenoid sinuses who have undergone transsphenoidal resections of skull base tumors and assess the complications and outcomes.
Methods Data was collected by a retrospective chart review done on children ages 7 and under who underwent endoscopic tumor resection and had a partially or completely nonpneumatized sphenoid sinus on preoperative computed tomography imaging. Surgical data collected included surgical corridor, gross total versus subtotal resection, repair method, use of septal flap, intraoperative and postoperative cerebrospinal fluid leak, and estimated blood loss.
Results Six patients were identified that fit our inclusion criteria who underwent surgery between November 2015 and April 2018 (3 males, 3 females; average age = 4.28 years). Tumor pathologies include three craniopharyngiomas, Rathke cleft cyst, meningocele, and neuroblastoma. All cases involved varying percentages of sphenoid sinus pneumatization. All cases except one craniopharyngioma and the neuroblastoma required removal of cancellous bone to access pathology. Degree of sphenoid pneumatization tended to be greater with age and resulted in less intraoperative bleeding. The two youngest patients with completely nonpneumatized sphenoid sinuses lost 61.73 and 17.52% of their total blood volume intraoperatively. Procedures were able to be adequately performed and pathology completely addressed with minimal postoperative complications and no postoperative CSF leaks.
Conclusions Intraoperative challenges including hemorrhage are likely in nonpneumatized patients, and thus surgeons should be prepared with adequate vascular access and blood products. Hemorrhage and total blood volume loss are increased in the younger patients with no sphenoid pneumatization. Despite the additional challenges, a nonpneumatized sphenoid sinus is not a contraindication for an endoscopic resection in the pediatric population.
On a Plasminogen Activator from Human Plasma
Authors: Diaz Batista A , Hernandez Solana G , Corral Almonte J F 
Published: February 2019

SummaryA plasminogen activating substance was purified from the dialysates of the eluates of glass adsorbed kallikrein from fresh human plasma, by chromatography on QAE-Sephadex A-50 and gel filtration in Sephadex G-25. The preparation was concentrated by lyophilization. Its electrophoretic mobility was found to be similar to that of prealbumin. Its molecular weight appeared to be 15000–18000 daltons. The analysis of aminoacids of this activator showed that it contains a high proportion of acid amino acids. The purified activator showed esterase activity, fibrinolytic activity and kininogenase activity on heated human plasma. These activities were respectively equivalent to 150 μM BAEe/mg protein, 19 × 103 units of streptokinase/μg protein and 250 μg bradykinin/mg protein.
Toward perfect reads: self-correction of short reads via mapping on de Bruijn graphs
Authors: Limasset Antoine , Flot Jean-François , Peterlongo Pierre 
Published: February 2019

Abstract

Motivation
Short-read accuracy is important for downstream analyses such as genome assembly and hybrid long-read correction. Despite much work on short-read correction, present-day correctors either do not scale well on large datasets or consider reads as mere suites of k-mers, without taking into account their full-length sequence information.

Results
We propose a new method to correct short reads using de Bruijn graphs and implement it as a tool called Bcool. As a first step, Bcool constructs a compacted de Bruijn graph from the reads. This graph is filtered on the basis of k-mer abundance then of unitig abundance, thereby removing most sequencing errors. The cleaned graph is then used as a reference on which the reads are mapped to correct them. We show that this approach yields more accurate reads than k-mer-spectrum correctors while being scalable to human-size genomic datasets and beyond.

Availability and implementation
The implementation is open source, available at http://github.com/Malfoy/BCOOL under the Affero GPL license and as a Bioconda package.

Supplementary information
Supplementary data are available at Bioinformatics online.