bluuebunny/crossref_metadata_2025_split huggingface dataset:
Initial Safety of Total Talus Replacement Used to Treat Talar Avascular Necrosis
Authors: Abar Bijan , Kim Michael S. , Adams Samuel B. , Adams William R. , Amendola Annunziato , Easley Mark E. , Ellington John Kent , Ford Samuel E. , Hanselman Andrew E. , Highlander Peter , Kwon John Y. , Miller Christopher P. , Nunley James A. , Parker Claire , Parekh Selene G. , Schweitzer Karl M. , Shawen Scott B. , Mann Tara , Kelly Cambre 
Published: October 2024

Background: Total talus replacement (TTR) implants are designed to replace the diseased talar anatomy, reduce pain, maintain ankle range of motion, and restore ankle function after conservative treatments have failed. Currently TTR implants are produced by 3D printing a patient-specific implant designed from the patient’s preoperative anatomy. TTR surgery using patient-specific implants is a relatively new technique that remains understudied in the literature. Therefore, the purpose of this investigation was to determine the early safety and potential benefit of the TTR implant in patients with talar avascular necrosis. Methods: This retrospective, multicenter, cohort study evaluates the safety and potential benefits of TTR using 3D-printed patient-specific implants across 4 US centers. The primary outcome was the occurrence of early adverse events after TTR surgery. Secondary outcomes including, pain, and physical function were assessed using the pain visual analog scale (VAS), and Patient-Reported Outcomes Measurement Information System (PROMIS) physical function (PF), respectively. Results: The study team analyzed 15 patients with more than 1 year of follow-up. The mean duration of follow-up was 25.9 months (range: 18.3-41 months). Although 33.3% (5 of 15) of patients experienced adverse events, primarily occurring within the initial 6 months postoperatively, 93% (14 of 15) of patients reported implant survivorship. Of the 5 cases (33.3%) resulting in an adverse event, 3 (60.0%) were determined to be unrelated to the subject device, 2 (40.0%) were determined to be possibly procedure-related, and none (0%) were determined to be device-related. Conclusion: Although further studies are needed to compare TTR with the standard of care, the results of this study demonstrate the relative early safety of TTR surgery using a 3D-printed implant for the treatment of challenging talar pathologies. A larger and longer clinical study is required to see if the efficacy of this approach will be statistically and clinically meaningful.
Intensity-Dependent Effects of Low-Frequency Subthreshold rTMS on Primary Motor Cortex Excitability and Interhemispheric Inhibition in Elderly Participants: A Randomized Trial
Authors: Wischnewski Miles , Edwards Lauren , Revill Kate P. , Drake Daniel , Hobbs Gerald , Buetefisch Cathrin M. 
Published: October 2024

Background Low-frequency repetitive transcranial magnetic stimulation (LF-rTMS) protocols targeting primary motor cortex (M1) are used in rehabilitation of neurological diseases for their therapeutic potential, safety, and tolerability. Although lower intensity LF-rTMS can modulate M1 neurophysiology, results are variable, and a systematic assessment of its dose effect is lacking. Objectives To determine the dose-response of LF-rTMS on stimulated and non-stimulated M1. Methods In a sham-controlled randomized double-blind crossover study the effect of LF-TMS protocols were determined in 20 right-handed older healthy participants. In 3 sessions, 1 Hz rTMS at 80% (rTMS80), 90% (rTMS90) of motor threshold or sham stimulation were applied to left upper extremity M1. Outcome measures were curve parameters of the stimulus-response curve (maximum motor evoked potential [MEPMAX], slope and the intensity to evoke 50% MEPMAX), short-interval intracortical inhibition (SICI), and interhemispheric inhibition (IHI). Results Within LF-rTMS sessions, rTMS90, increased MEPMAX in the stimulated M1. Furthermore, rTMS90, increased the slope in the non-stimulated M1. LF-rTMS effects on SICI were dependent on the participants’ baseline SICI, hemisphere, and intensity of conditioning pulse. Finally, rTMS90 increased whereas rTMS80 decreased IHI, for both IHI directions. These changes were dependent on baseline IHI and hemisphere and were no longer significant when baseline IHI was accounted for. Conclusions Intensity of subthreshold LF-rTMS has differential effects on excitation and inhibition of stimulated and non-stimulated M1. The effects were small and were only demonstrated within the LF-rTMS sessions but were not different when compared to sham. rTMS related changes in SICI and IHI were dependent on baseline level. ClinicalTrials.gov Identifier: NCT02544503, NCT01726218.
COLODETECT 1: comparative evaluation of endocuff with computer-aided detection versus computer-aided detection alone versus standard colonoscopy for enhancing adenoma detection rates during screening colonoscopy—a pilot study
Authors: Caillo Ludovic , Delliot Clément , Chevallier Thierry , Bourgaux Jean-Francois , Prost Ardavan , Brunaud-Gagniard Bénédicte , Phoutthasang Valérie , Clerc Clémentine , Borderie Thomas , Daniel Jules , Pouderoux Philippe , Debourdeau Antoine 
Published: October 2024

Background: Independent use of artificial intelligence with computer-aided detection (CADe) and Endocuff Vision (ECV) has demonstrated enhanced adenoma detection rates (ADRs). Objective: Our pilot study aimed to define the necessary participant number for future randomized controlled trials (RCTs) by comparing the ADR of combined CADe + ECV against CADe alone and standard colonoscopy. Design: This single-center pilot study retrospectively analyzed a prospectively maintained database, where patients underwent screening colonoscopies sequentially by standard method, CADe alone, and then CADe + ECV. Method: The allocation of the technique depended on the study period. Patients were randomly selected from the cohort to form three groups of 30 patients, with stratification based on factors influencing the ADR. The primary endpoint was the ADR. Results: From April to June 2021, 244 patients underwent screening colonoscopy. 198 were eligible, and after randomization, 90 patients were included across three groups (colonoscopy n = 30, CADe n = 30, CADe + ECV = 30). The ADR was higher in the CADe + ECV group compared to the CADe and colonoscopy groups: 60% versus 40%, and 30%, respectively ( p = 0.03). The number of polyps ⩽3 mm detected was greater in the CADe + ECV group ( n = 23) versus CADe ( n = 7) and colonoscopy ( n = 12) groups, respectively ( p = 0.03). CADe + ECV identified more polyps in the cecum/right colon ( n = 26) compared to CADe ( n = 18) and colonoscopy ( n = 12) groups ( p = 0.04), and in the left colon/sigmoid ( n = 14) compared to CADe ( n = 5) and colonoscopy ( n = 2) ( p = 0.02). Conclusion: These findings underscore the synergic potential of combining CADe with ECV to enhance ADR and enable us to perform sample size calculations for future RCTs. Registration: Clinical Trials number: NCT05080088. Registration 06/06/2021.
Use of an injection of aripiprazole given once every 2 months (Abilify Asimtufii®) in people with bipolar I disorder: a Plain Language Summary of Publication
Authors: McIntyre Roger S. , Such Pedro , Yildirim Murat , Madera-McDonough Jessica , Jin Na , Watkin Suzanne , Zhang Zhen , Larsen Frank , Harlin Matthew 
Published: October 2024

The purpose of this summary is to explain key findings from a study that included people with bipolar I disorder, as described in two separate articles (see the ‘Further Information’ section for more details). The study compared a new formulation of aripiprazole, given as an injection once every 2 months, with a once‑monthly injection of aripiprazole.
Ostial LCx: Achilles Heel of Left Main Bifurcation Angioplasty and How We Evolved in Its Management
Authors: Sharma Richa 
Published: October 2024

In-stent restenosis (ISR) within bifurcation lesions of the left main (LM) coronary artery (LMCA) is challenging due to its complex anatomy and critical function. This case series highlights 3 patients with LM bifurcation ISR (Medina 1,1,1) post-index intervention using different stent strategies as follows: Case 1 A 58-year-old male developed distal LM bifurcation stenosis after ostial left anterior descending artery (LAD) stenting. Mini-culotte bifurcation angioplasty effectively managed this. Case 2 A 62-year-old male presented with neo-atherosclerosis-induced distal LM bifurcation ISR post-provisional LM-left anterior descending artery (LAD) stenting. A single-stent crossover with a drug-coated balloon in a large side branch achieved optimal flow restoration. Case 3 A chronic total occlusion in the right coronary artery and distal LM bifurcation ISR due to stent overhang into LMCA in a 72-year-old male was resolved with a string culotte technique. These cases underscore ISR management strategies tailored to patient-specific anatomy and clinical needs, advocating advanced imaging techniques like intravascular ultrasound and optical coherence tomography for procedural precision and safety.
Safety of an Accelerated Ferric Gluconate Inpatient Infusion Regimen
Authors: Gray Erica , Parrott Tate , McCluggage Lauren 
Published: October 2024

Background: Inpatient use of intravenous iron has been increasing. Ferric gluconate is traditionally given once daily. Twice-daily dosing provides faster iron repletion, but there are limited data to support the safety of twice-daily dosing. Objective: The aim of this study was to investigate the safety of twice-daily dosing for ferric gluconate compared with daily dosing. Methods: This was an institutional review board-approved retrospective observational study of hospitalized adult patients who received intravenous ferric gluconate 250 mg daily or twice daily between January 1 and April 3, 2022. The primary composite safety outcome included hypotension, infusion reaction, rapid response alert, or escalation in level of care. Secondary outcomes included total amount of iron received, hospital length of stay, and changes in laboratory values. Results: A total of 126 patients were included in this study, with 63 patients in each group. The primary outcome occurred in 29 patients (46%) in the twice-daily group compared with 36 patients (57.1%) in the daily group (relative risk = 0.81; 95% CI, 0.57-1.13; P = 0.212). Changes in iron, hemoglobin, and transferrin saturation were similar between the 2 groups. Median length of stay was statistically shorter in the twice-daily group (7.79 days) compared with the daily group (12.9 days; p = 0.006). Conclusions: In this retrospective single-center study of hospitalized adult patients, those who received intravenous ferric gluconate twice daily did not experience an increased rate of a composite safety outcome of hypotension, infusion reactions, or escalation in level of care compared with those with daily dosing.
Current myeloproliferative neoplasm scoring systems for clinical practice
Authors: Pasquer Hélène , Kiladjian Jean-Jacques , Benajiba Lina 
Published: October 2024

Abstract
BCR::ABL1-negative myeloproliferative neoplasms (MPNs) are clonal hematologic malignancies that are caused by the proliferation of myeloid cells that harbor a JAK-STAT pathway activating driver mutation. MPN management recommendations are based on the evaluation of different risks to prevent disease evolution–associated events while preserving patients’ quality of life. Such risks can be common across all MPNs or specific to each subtype (polycythemia vera [PV], essential thrombocythemia [ET], prefibrotic myelofibrosis [MF], and primary MF). Patients with MF harbor the worse prognosis, and hematopoietic stem cell transplantation (HSCT) is the only curative treatment at the expense of a high rate of morbidity and mortality. Therefore, accurate scoring systems to estimate overall survival are crucial for the management of patients with MF and the selection for HSCT. In PV and ET, the prediction of vascular events is prioritized given their higher incidence and related morbidity and mortality. Finally, quality of life evaluation is important for all subtypes. To predict these risks and adapt MPN therapeutic strategies, clinical risk scores have been developed over the past decades and more recently have incorporated molecular risk factors for more accurate risk stratification. The large number of scoring systems available, combined with disease heterogeneity and the necessity to predict diverse outcomes, make it difficult for clinicians to choose the most appropriate score to evaluate their patients’ risk in 2024. Here, we provide an overview of MPN disease evolution–associated event incidence and conduct an exhaustive comparative review of the scoring systems currently available for each risk. Finally, we propose an algorithm for the use of these scores in clinical practice in each MPN subtype.
Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection
Authors: Yang Li , Yang Lingli , Zhao Chuanji , Bai Zetao , Xie Meili , Liu Jie , Cui Xiaobo , Bouwmeester Klaas , Liu Shengyi 
Published: October 2024

Abstract
Background
Rapeseed (Brassica napus L.) is an important oil and industrial crop worldwide. Black rot caused by the bacterial pathogen Xanthomonas campestris pv. campestris (Xcc) is an infectious vascular disease that leads to considerable yield losses in rapeseed. Resistance improvement through genetic breeding is an effective and sustainable approach to control black rot disease in B. napus. However, the molecular mechanisms underlying Brassica-Xcc interactions are not yet fully understood, especially regarding the impact of post-transcriptional gene regulation via alternative splicing (AS).

Results
In this study, we compared the AS landscapes of a susceptible parental line and two mutagenized B. napus lines with contrasting levels of black rot resistance. Different types of AS events were identified in these B. napus lines at three time points upon Xcc infection, among which intron retention was the most common AS type. A total of 1,932 genes was found to show differential AS patterns between different B. napus lines. Multiple defense-related differential alternative splicing (DAS) hub candidates were pinpointed through an isoform-based co-expression network analysis, including genes involved in pathogen recognition, defense signalling, transcriptional regulation, and oxidation reduction.

Conclusion
This study provides new insights into the potential effects of post-transcriptional regulation on immune responses in B. napus towards Xcc attack. These findings could be beneficial for the genetic improvement of B. napus to achieve durable black rot resistance in the future.
Response to "Neglecting normalization impact in semi-synthetic RNA-seq data simulation generates artificial false positives" and "Winsorization greatly reduces false positives by popular differential expression methods when analyzing human population samples"
Authors: Ge Xinzhou , Li Yumei , Li Wei , Li Jingyi Jessica 
Published: October 2024

AbstractTwo correspondences raised concerns or comments about our analyses regarding exaggerated false positives found by differential expression (DE) methods. Here, we discuss the points they raise and explain why we agree or disagree with these points. We add new analysis to confirm that the Wilcoxon rank-sum test remains the most robust method compared to the other five DE methods (DESeq2, edgeR, limma-voom, dearseq, and NOISeq) in two-condition DE analyses after considering normalization and winsorization, the data preprocessing steps discussed in the two correspondences.
Almost 40 years of studying homeobox genes in C. elegans
Authors: Kratsios Paschalis , Hobert Oliver 
Published: October 2024

ABSTRACT
Homeobox genes are among the most deeply conserved families of transcription factor-encoding genes. Following their discovery in Drosophila, homeobox genes arrived on the Caenorhabditis elegans stage with a vengeance. Between 1988 and 1990, just a few years after their initial discovery in flies and vertebrates, positional cloning and sequence-based searches showed that C. elegans contains HOX cluster genes, an apparent surprise given the simplicity and non-segmented body plan of the nematode, as well as many other non-clustered homeobox genes of all major subfamilies (e.g. LIM, POU, etc.). Not quite 40 years later, we have an exceptionally deep understanding of homeodomain protein expression and function in C. elegans, revealing their prevalent role in nervous system development. In this Spotlight, we provide a historical perspective and a non-comprehensive journey through the C. elegans homeobox field and discuss open questions and future directions.
Characterization of a rapid avoidance behavior in Manduca sexta larvae in response to noxious stimuli
Authors: Kondakath Gayathri , Trimmer Barry A. 
Published: October 2024

ABSTRACT
This study focuses on the nociceptive responses observed in the tobacco hornworm (Manduca sexta). While prior investigations have described the sensory neurons and muscle activation patterns associated with the ‘strike’ behavior, there remains a gap in our understanding of the alternative ‘withdrawal’ movement, wherein the animal bends its head and thorax away from the stimulus. Our results show that stimulus location determines which nocifensive behavior is elicited. Interestingly, stimulation of specific mid-body segments could result in either withdrawal or strike, indicating a decision process rather than a hard-wired circuit. The withdrawal behavior was characterized using high-speed videography and electromyography. The results show that withdrawal in M. sexta is driven by contralateral ventral muscles, followed by an increase in ipsilateral muscle activation just before the bending stops. Dorsal muscles are co-activated throughout the movement. Although both withdrawal and strike behaviors involve sequential activation of lateral muscles, these behaviors involve different muscle groups. This discovery provides a novel model system to investigate the context dependence and decision-making processes triggered by stressful or noxious stimuli.
The glycoprotein hormone receptor (LGR1) influences Malpighian tubule secretion rate in Rhodnius prolixus
Authors: Al-Dailami Areej N. , Lange Angela B. , Orchard Ian 
Published: October 2024

ABSTRACT
In the hemipteran Rhodnius prolixus, successful post-prandial diuresis is accomplished through the synergistic actions of the peptidergic diuretic hormone RhoprCRF/DH and the biogenic amine 5-hydroxytryptamine (5-HT), and by an antidiuretic hormone RhoprCAPA-2 that terminates diuresis by inhibiting this synergy. Lateral neurosecretory cells (NSCs) in the mesothoracic ganglionic mass release RhoprCRF/DH, while midline NSCs release RhoprCAPA-2 during blood feeding. These NSCs co-express GPA2/GPB5, a conserved glycoprotein hormone involved in various physiological processes across bilaterians. This study investigated the influence of GPA2/GPB5 signaling on Malpighian tubule (MT) fluid secretion in R. prolixus. GPB5-like immunoreactivity in lateral and midline NSCs decreased following a blood meal, suggesting release and a role in diuresis. Downregulating the GPA2/GPB5 receptor LGR1 via RNA interference resulted in an increased basal fluid secretion rate in MTs, which was inhibited by the antidiuretic hormone RhoprCAPA-2. dsLGR1 treatment reduced the effects of RhoprCRF/DH and 5-HT on MT secretion and eliminated their synergism. RT-qPCR revealed that the expression of the diuretic and antidiuretic hormone receptors decreased in MTs of dsLGR1-injected insects, indicating that GPA2/GPB5 influences the expression of these other receptors. Downregulating LGR1 resulted in a smaller blood meal size and disrupted the normal time course of diuresis. As LGR1 is the most abundantly expressed G protein-coupled receptor gene in R. prolixus MTs, our results suggest that GPA2/GPB5 signaling has a critical role in regulating the timing and success of water retention in the unfed state, and in the complex processes associated with feeding and diuresis in R. prolixus.
Bayesian Ensembles of Exponentially Smoothed Life-Cycle Forecasts
Authors: Guo Xiaojia , Lichtendahl Kenneth C. , Grushka-Cockayne Yael 
Published: October 2024

Problem definition: We study the problem of forecasting an entire demand distribution for a new product before and after its launch. Firms need accurate distributional forecasts of demand to make operational decisions about capacity, inventory, and marketing expenditures. We introduce a unified, robust, and interpretable approach to producing these pre- and postlaunch distributional forecasts. Methodology/results: Our approach is inspired by Bayesian model averaging. Each candidate model in our ensemble is a life-cycle model fitted to the completed life cycle of a comparable product. A prelaunch forecast is an ensemble with equal weights on the candidate models’ forecasts, whereas a postlaunch forecast is an ensemble with weights that evolve according to Bayesian updating. Our approach is part frequentist and part Bayesian, resulting in a novel approach tailored to the demand forecasting challenge. We also introduce a new type of life-cycle or product diffusion model with states that can be updated using exponential smoothing. The trend in this model follows the density of an exponentially tilted Gompertz random variable. For postlaunch forecasting, this model is attractive because it can adapt itself to the most recent changes in a product’s life cycle. We provide closed-form distributional forecasts from our model. In two empirical studies, we show that when the ensemble’s candidate models are all in our new type of exponential smoothing model, this version of the ensemble outperforms several leading approaches in both point and quantile forecasting. Managerial implications: In a data-driven operations environment, our model can produce accurate forecasts frequently and at scale. When quantile forecasts are needed, our model has the potential to provide meaningful economic benefits. In addition, our model’s interpretability should be attractive to managers who already use exponential smoothing and ensemble methods for other forecasting purposes. Supplemental Material: The online appendix is available at https://doi.org/10.1287/msom.2022.0359 .