bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parallel Analyses by Mass Spectrometry (MS) and Reverse Phase Protein Array (RPPA) Reveal Complementary Proteomic Profiles in Triple‐Negative Breast Cancer (TNBC) Patient Tissues and Cell Cultures
Authors: Wang Nan , Zhu Yiying , Wang Lianshui , Dai Wenshuang , Hu Taobo , Song Zhentao , Li Xia , Zhang Qi , Ma Jianfei , Xia Qianghua , Li Jin , Liu Yiqiang , Long Mengping , Ding Zhiyong 
Published: November 2024

ABSTRACTHigh‐plex proteomic technologies have made substantial contributions to mechanism studies and biomarker discovery in complex diseases, particularly cancer. Despite technological advancements, inherent limitations in individual proteomic approaches persist, impeding the achievement of comprehensive quantitative insights into the proteome. In this study, we employed two widely used proteomic technologies, mass spectrometry (MS) and reverse phase protein array (RPPA) to analyze identical samples, aiming to systematically assess the outcomes and performance of the different technologies. Additionally, we sought to establish an integrated workflow by combining these two proteomic approaches to augment the coverage of protein targets for discovery purposes. We used 14 fresh frozen tissue samples from triple‐negative breast cancer (TNBC: seven tumors versus seven adjacent non‐cancerous tissues) and cell line samples to evaluate both technologies and implement this dual‐proteomic strategy. Using a single‐step protein denaturation and extraction protocol, protein samples were subjected to reverse‐phase liquid chromatography (LC) followed by electrospray ionization (ESI)‐mediated MS/MS for proteomic profiling. Concurrently, identical sample aliquots were analyzed by RPPA for profiling of over 300 proteins and phosphoproteins that are in key signaling pathways or druggable targets in cancer. Both proteomic methods demonstrated the expected ability to differentiate samples by groups, revealing distinct proteomic patterns under various experimental conditions, albeit with minimal overlap in identified targets. Mechanism‐based analysis uncovered divergent biological processes identified with the two proteomic technologies, capitalizing on their complementary exploratory potential.
Knee joint mechanics during gait after anterior cruciate ligament reconstruction using a partial or full thickness quadriceps tendon autograft at 2 years after surgery
Authors: Ripic Zachary , Letter Michael , Parrino Rosalia , Adams William , Kaplan Lee D. , Baraga Michael G. , Best Thomas M. , Signorile Joseph F. , Eltoukhy Moataz 
Published: November 2024

AbstractBackgroundDespite quadriceps weakness in individuals after quadriceps tendon anterior cruciate ligament reconstruction (QT‐ACLR), and its association with knee joint mechanics, no studies have addressed gait mechanics in both partial‐thickness (PT‐Q) and full‐thickness (FT‐Q) options for QT‐ACLR.ObjectiveTo assess gait mechanics across a QT‐ACLR cohort. We hypothesized that QT‐ACLR would show changes in knee joint mechanics compared to control participants (CON) and nonoperated limbs. Additionally, we hypothesized that FT‐Q operated limbs would show greater changes compared to PT‐Q and CON.DesignRetrospective cohort study.SettingUniversity‐affiliated sports medicine institute.ParticipantsSixteen patients who underwent QT‐ACLR (7 FT‐Q: Age (years) = 28.6 ± 7.3, post‐op (months) = 23.5 ± 10.7, 9 PT‐Q: Age = 25.2 ± 4.3, post‐op = 24.4 ± 11.7) were recruited and compared to 11 CON (age = 23.4 ± 4.8).InterventionParticipants underwent gait testing with force plate integrated motion capture.Main Outcome MeasuresMixed repeated‐measures analyses of covariance, adjusted for gait speed, were used to determine significant main effects or interactions in peak knee flexion angle, sagittal knee range of motion, peak internal knee extension moment (KEM), and peak internal knee flexion moment.ResultsWhen measured an average of 2 years after surgery, no main effect for limb or limb by depth interaction were detected. A significant effect by group was observed for peak KEM (p = .03, η2 = .27) and peak knee flexion angle (p = .04, η2 = .24) in the loading response phase. FT‐Q (p = .02) and PT‐Q (p = .03) showed lower KEM compared to the CON group in both limbs. The FT‐Q group showed lower peak knee flexion angle compared to the CON group (p = .01).ConclusionsKnee joint symmetry may be recovered 2 years following QT‐ACLR, but lower KEM compared to CON for both graft options and lower peak knee flexion angle than CON for the FT‐Q group may indicate a need for further investigation in QT‐ACLR.
Superior Conductive, Large Diameter CNT Composite Yarn of Insulative Polymer: Inferences of a Unidirectional Compression‐Stretching Technique
Authors: Farha Farial Islam , Chen Wei , Xu Fujun 
Published: November 2024

ABSTRACTLarge diameter yet highly conductive Carbon Nanotube (CNT) yarn could have prospective control on high‐tech application field spanning from electronic devices to wearable textiles; although the development of such macroscopic CNTs is extremely challenging. Especially, while fabricating CNT composite yarn with polymer, insulative nature of polymeric materials inherently propagates poor electrical conductivity to CNT yarn. To address this, we have proposed an exciting approach to fabricate large dimension, highly conductive yet flexible CNT‐Thermoplastic Polyurethane (TPU) composite yarn through unidirectional compression‐stretching process. Our technique allowed TPU molecules to be well dispersed into inner and inter‐CNT bundles facilitating well flexibility while the simultaneous functions of pressure and tension allowed more closely packed CNTs network within densified CNT yarn reducing the inherent voids and contact resistance. The consequent yarn possessed high conductivity of 1613 S/cm, attractive mechanical performance (tensile strength 1.3 GPa, Young's modulus 12 GPa, toughness 100.75 MJ/m3), exceptional anti‐abrasive ability (up to 46,350 cycles) endowing its multidirectional adaptability for smart textiles. Moreover, the desirable E‐heating performance together with excellent electrical stability allows successful exploitation of the prepared CNT yarn as stretchable heater. Such amazing integrated characteristics may allow the resultant yarn to replace traditional carbon fiber in various high‐tech arena as well.
The potent PHL4 transcription factor effector domain contains significant disorder
Authors: Fonda Blake D. , Murray Dylan T. 
Published: November 2024

AbstractThe phosphate‐starvation response transcription‐factor protein family is essential to plant response to low‐levels of phosphate. Proteins in this transcription factor (TF) family act by altering various gene expression levels, such as increasing levels of the acid phosphatase proteins which catalyze the conversion of inorganic phosphates to bio‐available compounds. There are few structural characterizations of proteins in this TF family, none of which address the potent TF activation domains. The phosphate‐starvation response‐like protein‐4 (PHL4) protein from this family has garnered interest due to the unusually high TF activation activity of the N‐terminal domain. Here, we demonstrate using solution nuclear magnetic resonance (NMR) measurements that the PHL4 N‐terminal activating TF effector domain is mainly an intrinsically disordered domain of over 200 residues, and that the C‐terminal region of PHL4 is also disordered. Additionally, we present evidence from size‐exclusion chromatography, diffusion NMR measurements, and a cross‐linking assay suggesting full‐length PHL4 forms a trimeric or tetrameric assembly. Together, the data indicate the N‐ and C‐terminal disordered domains in PHL4 flank a central folded region that likely forms the ordered oligomer of PHL4. This work provides a foundation for future studies detailing how the conformations and molecular motions of PHL4 change as it acts as a potent activator of gene expression in phosphate metabolism. Such a detailed mechanistic understanding of TF function will benefit genetic engineering efforts that take advantage of this activity to boost transcriptional activation of genes across different organisms.
Novel dysregulated long non‐coding RNAs in the acute kidney injury‐to‐chronic kidney diseases transition unraveled by transcriptomic analysis
Authors: Puri Bhupendra , Majumder Syamantak , Gaikwad Anil Bhanudas 
Published: November 2024

AbstractAcute kidney injury (AKI)‐to‐chronic kidney disease (CKD) transition involves a complex pathomechanism, including inflammation, apoptosis, and fibrosis where long non‐coding RNAs (lncRNAs) play a crucial role in their regulation. However, to date, only a few lncRNAs have been discovered to be involved in the AKI‐to‐CKD transition. Therefore, this study aims to investigate the dysregulated lncRNAs in the AKI‐to‐CKD transition in vitro and in vivo. To mimic AKI‐to‐CKD transition both in vivo and in vitro, bilateral ischemia‐reperfusion (IR) kidney injury was performed in Wistar rats (male), and normal rat kidney epithelial cell (NRK52E) cells were treated with exogenous transforming growth factor‐β1 (TGF‐β1). Further processing and analysis of samples collected from these studies (e.g., biochemical, histopathology, immunofluorescence, and RNA isolation) were also performed, and transcriptomic analysis was performed to identify the dysregulated lncRNAs. Rats subjected to IR showed a significant increase in kidney injury markers (creatinine, blood urea nitrogen (BUN), kidney injury molecule‐1(KIM‐1), and neutrophil gelatinase‐associated lipocalin (NGAL) along with altered cell morphology). Apoptosis, inflammation, and fibrosis markers were markedly increased during the AKI‐to‐CKD transition. Furthermore, transcriptomic analysis revealed 62 and 84 unregulated and 95 and 92 downregulated lncRNAs in vivo and in vitro, respectively. Additionally, functional enrichment analysis revealed their involvement in various pathways, including the tumor necrosis factor (TNF), wingless‐related integration site (Wnt), and hypoxia‐inducible factor‐1 (HIF‐1) signaling pathways. These identified dysregulated lncRNAs significantly contribute to AKI‐to‐CKD transition, and their knockin/out can aid in developing targeted therapeutic interventions against AKI‐to‐CKD transition.
Hydrogen‐Bonded Organic Framework and Metal–Organic Framework Assembly of Waterwheel PgC‐Noria Molecule: Regulating Microstructure Enables Iodine Transfer
Authors: Ren Wei‐Bo , Fu Yaomei , Zheng Haiyan , Hou Baoshan , Cui Dongxu , Zhao Liang , Zang Hong‐Ying , Wang Xinlong 
Published: November 2024

AbstractHydrogen‐bonded organic frameworks (HOFs) are a type of crystalline porous materials self‐assembled from organic or metal–organic building blocks via intermolecular hydrogen bonding, which have received increasing attention due to their reversible and flexible hydrogen bonding properties. Currently, it remains a challenge to construct HOFs based on complex or porous organic cages as molecular building blocks. Herein, a 3D HOF (PgC‐HOF) featuring honeycomb‐shaped channels is crafted utilizing a sizable waterwheel‐like PgC‐noria organic molecule cage. The pivotal role of intermolecular multipoint hydrogen bonding interactions in upholding structural integrity and stability is underscored by the possession of 36 phenolic hydroxyl groups in PgC‐HOF. Interestingly, the introduction of calcium ions into the reaction system results in the formation of the metal–organic framework (PgC‐MOF), with the channel dimensions increasing from 6.8 to 9.1 Å. Furthermore, I2 sorption/release experiments are conducted on PgC‐HOF and PgC‐MOF, achieving an increase in the optimal adsorption amount from 1.45 to 2.19 g g−1 and a transition from an irreversible adsorbent to a reversible adsorbent.
Construction and Multifunctional Photonic Applications of Light Absorption‐Enhanced Silicon‐Based Schottky Coupled Structures
Authors: Wu Huijuan , Lian Shanshui , Zhang Jinqiu , Wang Bingkun , Bai Wenjun , Ding Guqiao , Yang Siwei , Liu Zhiduo , Zheng Li , Ye Caichao , Wang Gang 
Published: November 2024

AbstractTo expand the detection capabilities of silicon (Si)‐based photodetector and address key scientific challenges such as low light absorption efficiency and short carrier lifetime in Si‐based graphene photodetector. This work introduces a novel Si‐based Schottky coupled structure by in situ growth of 3D‐graphene and molybdenum disulfide quantum dots (MoS2 QDs) on Si substrates using chemical vapor deposition (CVD) and plasma‐enhanced chemical vapor deposition (PECVD) techniques. The findings validate the “dual‐enhanced absorption” effect, enhancing the understanding of the mechanisms that improve optoelectronic performance. The synergistic effect of 3D‐graphene's natural nano‐resonant cavity and MoS2 QDs enhances light absorption efficiency and extends carrier lifetime. Introducing MoS2 QDs broadens and intensifies the built‐in electric field, promoting the separation of photogenerated electrons and holes. The photodetector exhibits a wideband light response in the wavelength range of 380–2200 nm. It stably outputs photocurrent under high‐frequency (1 kHz) modulated laser (2200 nm), with a responsivity (R) of 40 mA W−1 and detectivity (D*) of 1.15 × 109 Jones. Photodetectors show the ability to process and encrypt complex binary signals and achieve versatility in “AND” gate and “OR” gate logic operations, as well as image sensing (240 × 200 pixels).
Polar Molecule Intercalation to Weaken P2─S Bonding in MnPS3 Toward Ultrahigh‐Capacity Sodium Storage
Authors: Tu Xueyang , Fan Ke , Peng Baixin , Zhang Shaoning , Ma Yiran , Fang Yuqiang , Huang Haitao , Huang Fuqiang 
Published: November 2024

AbstractLayered transition metal trithiophosphates (TMPS3, TM = Mn, Fe, Co, etc.) with high theoretical capacity (>1 300 mAh g−1) are potential anode materials for sodium‐ion batteries (SIBs). However, the strong bonding between P2 dimers and S atoms in TMPS3 hinders the efficient alloying reaction between P2 dimers and Na+, resulting in practical capacities much lower than theoretical values. Herein, a polar molecule diisopropylamine (DIPA) is intercalated into MnPS3 for the first time to improve the sodium storage performance effectively. Theoretical calculations show that the electron transfer between DIPA and MnPS3 induces more delocalized S p states and weaker P─S bonds, significantly enhancing the electrochemical activity and sodiation/desodiation reaction kinetics. Moreover, the expanded interlayer spacing from 6.48 to 10.75 Å enables faster Na+ diffusion and more active sites for Na+ adsorption. As expected, the DIPA‐MnPS3 exhibits an ultrahigh capacity of 1,023 mAh g−1 at 0.2 A g−1 and excellent cycling performance (≈100% capacity retention after 4 200 cycles at 10 A g−1), far outperforming those metal thiophosphates anodes reported for SIBs. Interestingly, in situ and ex situ characterizations reveal a quasi‐topological intercalation mechanism of DIPA‐MnPS3. This work provides a novel strategy for the design of high‐performance anode materials for SIBs.
Controllable Ultrathin Nickel Nanoislands With Dense Discrete Space Charge Regions: Steering Hole Extraction for High‐Performance Underwater Multispectral Weak‐Light Photodetection
Authors: Wang Shuijing , Miao Tangying , Wang Yang , Xu Jinshan , Jia Fengyuan , Li Yang , Kou Jiahui , Xu Zhongzi 
Published: November 2024

AbstractUndersea optical communication (UOC) is vital for ocean exploration and military applications. In the dim‐light underwater environment, photodetectors must maximize photon utilization by minimizing optical losses and carrier recombination. This can be achieved by integrating ultrathin metal nanostructures with photocatalysts to form Schottky junctions, which enhance charge separation and injection while mitigating metal‐induced light shading. The strategic design of discrete metal nanostructures providing numerous high‐depth space charge regions (SCRs) without overlap offers a promising approach to optimize hole transport paths and further suppress recombination. Here, a facile phase‐separation lithography technique is explored to fabricate tunable ultrathin Ni nanoislands atop n‐Si, yielding high‐performance photoelectrochemical photodetectors (PEC PDs) tailored for underwater weak‐light environments. This results indicate that key determinant of hole extraction behavior is the relationship between the spacing distance of adjacent Ni nanostructures (ds) and twice the SCR radius (Ws). PEC PDs with optimized 8 nm ultrathin Ni nanostructures featuring closely but non‐overlapping SCRs, exhibit a 55‐fold increase in photoresponsivity (2.2 mA W−1) and a 128‐fold enhancement in detection sensitivity (3.2 × 1011 Jones) at 0 V over Ni film, revealing the exceptional stability. Furthermore, this approach enables effective detection across UV–vis‐near infrared spectrum, supporting reliable multispectral UOC and underwater imaging capabilities.
Dynamic Self‐Healing of the Reconstructed Phase in Perovskite Oxides for Efficient and Stable Electrocatalytic OER
Authors: Zhai Yiyue , Ren Xiangrong , Zhang Jing , Gan Tao , Yang Na , Wang Bolun , Liu Shengzhong (Frank) 
Published: November 2024

AbstractNeither electrocatalytic activity nor structural stability is inconsequential in water electrolysis. Unfortunately, they have to be compromised in practice, especially in the anodic redox chemistry of lattice oxygen. Herein, the discovery of a La1−xCexFeO3 perovskite is presented which shows both good stability and high catalytic activity. Using advanced operando characterizations, it is identified that the self‐healing evolution of the La1−xCexFeO3 perovskite plays a key role during water oxidation in the lattice oxygen‐mediated mechanism (LOM) pathway. Unlike irreversible reconstruction, the formation of reconstructed active‐phase α‐FeOOH is reversed by re‐crystallization of surface La1−xCexFeO3 upon return to noncatalytic conditions. The self‐healing transformation of the α‐FeOOH termination layer on the stable La1−xCexFeO3 core imparts remarkable long‐term stability as well as excellent electrocatalytic performance. As a result, a composition La0.9Ce0.1FeO3@FeOOH is designed that exhibits ultralow overpotentials of 257 and 312 mV to achieve 10 and 100 mA cm−2, respectively. The findings provide insight into self‐healing behavior toward engineering perovskite oxides for efficient and stable oxygen electrocatalysis.
Probing the Interplay of Protein Self‐Assembly and Covalent Bond Formation in Photo‐Crosslinked Silk Fibroin Hydrogels
Authors: Tran Hien A. , Maraldo Anton , Ho Trinh Thi‐Phuong , Thai Mai Thanh , van Hilst Quinn , Joukhdar Habib , Kordanovski Marija , Sahoo Jugal Kishore , Hartsuk Onur , Santos Miguel , Wise Steven G. , Kaplan David L. , Do Thanh Nho , Kilian Kristopher A. , Lim Khoon S. , Rnjak‐Kovacina Jelena 
Published: November 2024

AbstractCovalent crosslinking of silk fibroin via native tyrosine residues has been extensively explored; however, while these materials are very promising for biomedical, optical, soft robotics, and sensor applications, their structure and mechanical properties are unstable over time. This instability results in spontaneous silk self‐assembly and stiffening over time, a process that is poorly understood. This study investigates the interplay between self‐assembly and di‐tyrosine bond formation in silk hydrogels photo‐crosslinked using ruthenium (Ru) and sodium persulfate (SPS) with visible light. The effects of silk concentration, molecular weight, Ru/SPS concentration, and solvent conditions are examined. The Ru/SPS system enables rapid crosslinking, achieving gelation within seconds and incorporating over 90% of silk into the network, even at very low protein concentrations (≥0.75% wt/v). A model emerges where silk self‐assembly both before and after crosslinking affects protein phase separation, mesoscale structure, and dynamic changes in the hydrogel network over time. Silk concentration has the greatest impact on hydrogel properties, with higher silk concentration hydrogels experiencing two orders of magnitude increase in stiffness within 1 week. This new understanding and ability to tune hydrogel properties and dynamic stiffening aids in developing advanced materials for 4D biofabrication, sensing, 3D cancer models, drug delivery, and soft robotics.
Charge Separation‐Engineered Piezoelectric Ultrathin Nanorods Modulate Tumor Stromal Microenvironment and Enhance Cell Immunogenicity for Synergistically Piezo‐Thermal‐Immune Therapy
Authors: Wang Qian , Du Jun , Yang Fujun , Wu Sijia , Zhu Luna , Li Xueyu , Yang Han , Miao Yuqing , Li Yuhao 
Published: November 2024

AbstractThe tumor microenvironment (TME) is characterized by hypoxia and low immunogenicity, with a dense and rigid extracellular matrix (ECM) that impedes the diffusion of therapeutic agents and immune cells, thereby limiting the efficacy of immunotherapy. To overcome these challenges, an oxygen defect piezoelectric‐photothermal sensitizer, bismuth vanadate nanorod‐supported platinum nanodots (BVP) is developed. The integration of platinum enhances the photothermal effect and improves charge separation efficiency under ultrasound, leading to increased heat generation and the production of reactive oxygen species (ROS) and oxygen. Platinum also catalyzes the conversion of hydrogen peroxide in the TME to oxygen, which serves as both a ROS source and a means to alleviate tumor hypoxia, thereby reversing the immunosuppressive TME. Moreover, the coordination of bismuth ions with glutathione further amplifies cellular oxidative stress. The generated heat and ROS not only denature the collagen in the ECM, facilitating the deeper penetration of BVP into the tumor but also induce immunogenic cell death in tumor cells. Through the “degeneration and penetration” strategy, photoacoustic therapy effectively activates immune cells, inhibiting both tumor growth and metastasis. This study introduces a pioneering approach in the design of antitumor nanomedicines aimed at reversing the immunosuppressive characteristics of the TME.
Synthesis Framework for Designing PtPdCoNiMn High‐Entropy Alloy: A Stable Electrocatalyst for Enhanced Alkaline Hydrogen Evolution Reaction
Authors: Chandran M Athira , Sahoo Sudeshna , Singh Ashutosh K. , Prasad Bhagavatula L. V. 
Published: November 2024

AbstractHigh entropy alloys (HEAs) are an emerging class of advanced materials characterized by their multifunctionality and potential to replace commercial catalysts in electrocatalytic water splitting. The synergy among the various alloyed elements in HEAs makes them particularly promising for applications in electrocatalysis. However, preparation of HEA via bottom‐up approaches by avoiding the formation of mono, di, and tri metallic alloys in the nanoscale is challenging. This aspect is addressed, in this study by exploring the logical selection of solvents, reducing agents, and capping agents, along with their relative fractions, in the solvothermal synthesis of the HEA comprising platinum‐palladium‐cobalt‐nickel‐manganese (PtPdCoNiMn). It is established that the reducing capabilities of both the solvent and reducing agent are crucial for the reduction of each metal to form a single‐phase HEA. The synthesized HEA (20 wt.%)/functionalized carbon (FC) demonstrates excellent performance as an HER catalyst, exhibiting a low overpotential of 48.7 mV at −10 mA cm−2 in an alkaline electrolyte. This performance is characterized by high reaction kinetics and stability at elevated current densities. Furthermore, the catalyst shows impressive performance in both simulated and actual seawater. This development reduces the reliance on platinum while enhancing the long‐term durability and catalytic efficiency of the electrocatalyst.