bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human-Induced Pluripotent Stem Cell Model of Trastuzumab-Induced Cardiac Dysfunction in Patients With Breast Cancer
Authors: Kitani Tomoya , Ong Sang-Ging , Lam Chi Keung , Rhee June-Wha , Zhang Joe Z. , Oikonomopoulos Angelos , Ma Ning , Tian Lei , Lee Jaecheol , Telli Melinda L. , Witteles Ronald M. , Sharma Arun , Sayed Nazish , Wu Joseph C. 
Published: March 2019

Background:
Molecular targeted chemotherapies have been shown to significantly improve the outcomes of patients who have cancer, but they often cause cardiovascular side effects that limit their use and impair patients’ quality of life. Cardiac dysfunction induced by these therapies, especially trastuzumab, shows a distinct cardiotoxic clinical phenotype in comparison to the cardiotoxicity induced by conventional chemotherapies.

Methods:
We used the human induced pluripotent stem cell–derived cardiomyocyte (iPSC-CM) platform to determine the underlying cellular mechanisms in trastuzumab-induced cardiac dysfunction. We assessed the effects of trastuzumab on structural and functional properties in iPSC-CMs from healthy individuals and performed RNA-sequencing to further examine the effect of trastuzumab on iPSC-CMs. We also generated human induced pluripotent stem cells from patients receiving trastuzumab and examined whether patients’ phenotype could be recapitulated in vitro by using patient-specific iPSC-CMs.

Results:
We found that clinically relevant doses of trastuzumab significantly impaired the contractile and calcium-handling properties of iPSC-CMs without inducing cardiomyocyte death or sarcomeric disorganization. RNA-sequencing and subsequent functional analysis revealed mitochondrial dysfunction and altered the cardiac energy metabolism pathway as primary causes of trastuzumab-induced cardiotoxic phenotype. Human iPSC-CMs generated from patients who received trastuzumab and experienced severe cardiac dysfunction were more vulnerable to trastuzumab treatment than iPSC-CMs generated from patients who did not experience cardiac dysfunction following trastuzumab therapy. It is important to note that metabolic modulation with AMP-activated protein kinase activators could avert the adverse effects induced by trastuzumab.

Conclusions:
Our results indicate that alterations in cellular metabolic pathways in cardiomyocytes could be a key mechanism underlying the development of cardiac dysfunction following trastuzumab therapy; therefore, targeting the altered metabolism may be a promising therapeutic approach for trastuzumab-induced cardiac dysfunction.
Is Marriage Incompatible with Political Liberalism?
Authors: Toop Alison 
Published: March 2019

This paper examines three arguments that claim marriage, as a political institution, is incompatible with political liberalism. These arguments are drawn from Elizabeth Brake
1
and Clare Chambers.
2
My purpose here is to determine which, if any, of the arguments show marriage to be incompatible with political liberalism.
The “Neutrality Argument” claims that the political institution of marriage violates the political liberal principle of neutrality. I claim that no such violation occurs. The “Unjustified Discrimination Argument” alleges that marriage involves the state in unjustified discrimination. I suggest there are grounds for the differential treatment identified. The “Public Reason Argument” argues that marriage, as it is currently structured, violates the political liberal principle of public reason. I claim that its current structure can be justified by appeal to public reasons. I therefore conclude that none of these arguments successfully demonstrate that marriage is incompatible with political liberalism.
Escherichia coli-induced granulomatous colitis in a cat
Authors: Matsumoto Isao , Nakashima Ko , Morita Hajime , Kasahara Koichi , Kataoka Osamu , Uchida Kazuyuki 
Published: March 2019

Case summary A 10-year-old castrated male domestic shorthair cat presented with a 6 month history of diarrhoea that responded poorly to medical treatment. Ultrasonography revealed moderate thickening of the colonic wall (4.8 mm) and right colic and jejunal lymphadenomegalies. Endoscopic examination revealed partial circumferential narrowing of the transverse colon and friable colonic mucosa with multiple haemorrhagic regions. Histopathological and immunohistochemical examinations revealed a large number of Escherichia coli phagocytosed by periodic acid–Schiff-positive macrophages. Bacterial culture also yielded enrofloxacin-sensitive E coli. The cat was initially treated with prednisolone, which resulted in little improvement. Following histopathological examination and bacterial culture, treatment with enrofloxacin was commenced. Antibacterial therapy resulted in remission of the diarrhoea and an increase in body weight within 14 days. Relevance and novel information Granulomatous colitis (GC) or histiocytic ulcerative colitis has been rarely described in cats. There has only been one previously published case study involving a cat, and the aetiology remains largely unknown. The current article describes the regression of E coli-related GC following antibacterial treatment in a cat. Clinical signs, histopathological appearance and response to enrofloxacin were similar to those in canine GC. The current findings suggest that E coli also plays an important role in the development of feline GC.
Patient-Reported Satisfaction Following Radiation of Implant-Based Breast Reconstruction
Authors: Thiboutot Eva , Craighead Peter , Webb Carmen , Temple-Oberle Claire 
Published: March 2019

Background: Little is known with regard to patient-reported outcomes (PROs) in the setting of implant-based reconstruction (IBR) with post-mastectomy radiation therapy (PMRT). Methods: We identified patients who underwent immediate IBR from a prospectively compiled database. The Breast Reconstruction Satisfaction Questionnaire (BRECON-31) was scored and compared between patients with and without PMRT. Results: Sixty-four women met the study criteria. Forty-eight did not receive PMRT and 16 did. Nine women had an unanticipated indication for PMRT. The PMRT group was similar to the control group with regard to baseline characteristics (ie, age, marital status, body mass index, tobacco use, and comorbidities). However, treatment and oncologic characteristics (eg, diagnosis, tumour characteristics, systemic therapy use) differed. Of all complications, only capsular contracture rates differed (1.2% vs 13%; P = .01). Of the 9 subscales, 7 showed no difference in satisfaction between the groups. Radiated women scored lower in the arm concerns and breast appearance subscales. Scores were similar whether the indication for PMRT had been anticipated or not. Discussion: Women with immediate IBR scored similarly to their nonradiated counterparts across 7 of 9 domains of satisfaction. Arm concerns and breast appearance scores are lower with PMRT, likely secondarily to more extensive nodal procedures in higher stage patients and to the side effect profile of radiotherapy. Our findings are in line with the few available studies using other PRO tools to evaluate the impact of PMRT on patient satisfaction and studies objectively measuring the effect of PMRT on arm morbidity and cosmetic outcomes.
The Association Between Facial Proportions and Patient Satisfaction After Rhinoplasty: A Prospective Study
Authors: Zojaji Ramin , Sobhani Ehsan , Keshavarzmanesh Mojdeh , Dehghan Parastoo , Meshkat Mojtaba 
Published: March 2019

Hypothesis: Rhinoplasty is one of the most common aesthetic surgeries. The aim of the study was to evaluate facial proportions and patient satisfaction with the appearance of their nose after rhinoplasty compared to preoperatively. Methods: This cross-sectional study was conducted at Islamic Azad University, Mashhad Branch. Eighty-two candidates with indications for primary cosmetic rhinoplasty were selected. Facial proportions and patient satisfaction with their nasal appearance were evaluated before and 6 months after rhinoplasty. Patient satisfaction was assessed using the Rhinoplasty Outcome Evaluation questionnaire. Results: Eighty-two patients referred for cosmetic rhinoplasty (13.4% men and 86.6% women) with a mean age of 28.5 (6.4) years underwent open rhinoplasty and were evaluated. Facial proportions, including nasofrontal, nasolabial, and nasomental ratios increased significantly, while the nasofacial ratio, nasal tip projection, columellar show, alar base, and nasal length reduced significantly after rhinoplasty ( P < .05). Furthermore, patient satisfaction with the appearance of their nose increased significantly after surgery ( P < .001). Conclusion: All of the facial proportions changed significantly following rhinoplasty. Although patient satisfaction with nose appearance increases significantly after the operation, there is no significant association between patient satisfaction and facial proportions. As beauty is a subjective, relative and qualitative issue, facial proportions may not be a proper tool to determine facial beauty and predict the satisfaction rate following rhinoplasty.
Infantile myofibromatosis treated by mandibulectomy and staged reconstruction with submental flap and free fibula flap: A case report
Authors: Maby Alexandra , Guay Benoit , Thuot François 
Published: March 2019

Background Infantile myofibromatosis is the most common benign fibrous tumor in infants. Three different types have been reported in the literature. The most commonly affected areas are the head, the neck and the trunk. Our patient showed a very high level of mandibular destruction resistant to all mandibular sparing treatment strategies requiring segmental mandibulectomy and complex reconstruction. Case presentation We describe a rare case of multicentric infantile myofibromatosis with mandibular bone destruction. The treatment required a succession of chemotherapy, a subtotal transoral resection and a hemi-mandibulectomy. The mandibular reconstruction was staged with initial bridging titanium plate with a submental flap, followed later by a fibula free flap. Conclusion Mandibular involvement by myofibromatosis is rare, and the extend of bone destruction and reconstruction make this case unique. To our knowledge, this is the only reported case of fibula free flap mandibular reconstruction in a patient with infantile myofibromatosis , as well as one of the youngest reported submental island flaps for any pathology. We describe the clinical presentation and management, including relevant imaging, histopathology, medical and surgical treatment as well as a review of relevant literature.
MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex
Authors: Capmany Anahi , Yoshimura Azumi , Kerdous Rachid , Caorsi Valentina , Lescure Aurianne , Del Nery Elaine , Coudrier Evelyne , Goud Bruno , Schauer Kristine 
Published: March 2019

ABSTRACT
In this study, we aimed to identify the myosin motor proteins that control trafficking at the Golgi complex. In addition to the known Golgi-associated myosins MYO6, MYO18A and MYH9 (myosin IIA), we identified MYO1C as a novel player at the Golgi in a human cell line. We demonstrate that depletion of MYO1C induces Golgi complex fragmentation and decompaction. MYO1C accumulates at dynamic structures around the Golgi complex that colocalize with Golgi-associated actin dots. MYO1C depletion leads to loss of cellular F-actin, and Golgi complex decompaction is also observed after inhibition or loss of the actin-related protein 2/3 complex, Arp2/3 (also known as ARPC). We show that the functional consequence of MYO1C depletion is a delay in the arrival of incoming transport carriers, both from the anterograde and retrograde routes. We propose that MYO1C stabilizes actin at the Golgi complex, facilitating the arrival of incoming transport carriers at the Golgi.
This article has an associated First Person interview with the first author of the paper.
Intraoperative evaluation of blood flow for soft tissues in orthopaedic surgery using indocyanine green fluorescence angiography
Authors: Doi N. , Izaki T. , Miyake S. , Shibata T. , Ishimatsu T. , Shibata Y. , Yamamoto T. 
Published: March 2019

Objectives Indocyanine green (ICG) fluorescence angiography is an emerging technique that can provide detailed anatomical information during surgery. The purpose of this study is to determine whether ICG fluorescence angiography can be used to evaluate the blood flow of the rotator cuff tendon in the clinical setting. Methods Twenty-six patients were evaluated from October 2016 to December 2017. The participants were categorized into three groups based on their diagnoses: the rotator cuff tear group; normal rotator cuff group; and adhesive capsulitis group. After establishing a posterior standard viewing portal, intravenous administration of ICG at 0.2 mg/kg body weight was performed, and fluorescence images were recorded. The time from injection of the drug to the beginning of enhancement of the observed area was measured. The hypovascular area in the rotator cuff was evaluated, and the ratio of the hypovascular area to the anterolateral area of the rotator cuff tendon was calculated (hypovascular area ratio). Results ICG fluorescence angiography allowed for visualization of blood flow in the rotator cuff in all groups. The adhesive capsulitis group showed significantly earlier enhancement than the other groups. Furthermore, the adhesive capsulitis group had a significantly smaller hypovascular area ratio than the other groups. Conclusion ICG fluorescence angiography allowed for evaluation of real-time blood flow of the rotator cuff in arthroscopic shoulder surgery. The techniques of ICG fluorescence angiography are simple and easy to observe, observer reliability is high, and it has utility for evaluating blood flow during surgery. Cite this article: N. Doi, T. Izaki, S. Miyake, T. Shibata, T. Ishimatsu, Y. Shibata, T. Yamamoto. Intraoperative evaluation of blood flow for soft tissues in orthopaedic surgery using indocyanine green fluorescence angiography: A pilot study. Bone Joint Res 2019;8:118–125. DOI: 10.1302/2046-3758.83.BJR-2018-0151.R1.
Generation of Highly Biomimetic Quality Control Materials for Noninvasive Prenatal Testing Based on Enzymatic Digestion of Matched Mother–Child Cell Lines
Authors: Zhang Rui , Ding Jiansheng , Gao Peng , Li Ziyang , Tan Ping , Li Jinming 
Published: March 2019

Abstract

BACKGROUND
Noninvasive prenatal testing (NIPT) based on cell-free DNA (cfDNA) is widely used. However, biomimetic quality control materials that have properties identical to clinical samples and that are applicable to a wide range of methodologies are still not available to support assay development, internal quality control, and proficiency testing.

METHODS
We developed a set of dual enzyme-digested NIPT quality control materials (DENQCMs) that comprise simulated human plasma and mixtures of mother cell line-derived cfDNA based on DNA fragmentation factor digestion (D-cfDNA) and the matched child cell line-derived cfDNA based on micrococcal nuclease digestion (M-cfDNA). Serially diluted samples positive for trisomies 21, 18, and 13 were included in the materials. To evaluate the biomimetics, DENQCMs were analyzed using random massively parallel sequencing (MPS), targeted MPS, and imaging single DNA molecule methods, and the estimated fetal fractions (FFs) were compared with expected FFs. Genome-wide analysis of cfDNA fragmentation patterns was performed to confirm their biological characteristics.

RESULTS
The genetic status of each DENQCM was correctly detected by 4 routine NIPT assays for the samples with FFs &gt;5%. The chromosome Y-based and single-nucleotide polymorphism-based estimations of FFs were linearly related to those expected FFs. The MPS results exhibited a concordance of quality metrics between DENQCMs and maternal plasma, such as GC contents of cfDNA and unique read ratios.

CONCLUSIONS
The DENQCMs are universally applicable for different platforms. We propose DENQCMs as an approach to produce matched maternal and fetal cfDNA that will be suitable for the preparation of quality control materials for NIPT.
De la asignatura al taller de literatura como estrategia didáctica
Authors: Triana Castro Yesny Milena 
Published: March 2019

Este artículo es el resultado de una intervención pedagógica realizada en la Escuela Normal Superior Distrital María Montessori (ENSDMM) de Bogotá, D.C., con estudiantes de grado undécimo durante el segundo semestre de 2015 en el marco de un proyecto de aula titulado “Ritos y rituales, un viaje por el mundo a través de la literatura universal”, en el que participó un equipo de maestros en formación de la Licenciatura en Educación Básica con énfasis en Humanidades y Lengua Castellana de la Universidad Distrital Francisco José de Caldas. El trabajo confirma como problema la necesidad de movilizar la concepción de asignaturade español como punto donde se imparten saberes acerca de la lengua y la literatura, al de taller literario, como espacio para la creación colectiva y la reflexión crítica conjunta. Se evidencia entonces, una propuesta para la mejora de la enseñanza de la literatura, en la que se presenta, en orden, el diseño del taller de literatura, la importancia de un enfoque curricular y la sistematización de la experiencia del maestro en formación. Se concluye que el taller literario puede llegar a reemplazar varios componentes de la asignatura de español ya que efectivamente permite la construcción de conocimiento tanto del maestro como del estudiante. del estudiante.
Analyzing the dynamics in the attitudes of youth in the post-Soviet countries towards CIS intergration processes
Authors: Karabchuk Tatiana S. , Poplavskaya Anita A. 
Published: March 2019

The paper provides an analysis of the dynamics of youth perceptions of integration processes in the post-Soviet countries. The study is focused on the opinions and preferences of young persons aged 18-24 living in Armenia, Belarus, Kazakhstan, Kyrgyzstan, Moldova, Russia and Tajikistan. Which countries are the most attractive for the young generations to live, work and study? Which countries are good to develop economic relations with, to exchange technologies with and to attract specialists from? What attitudes do youngsters have towards Russia, the U.S.A. and China? What do young people think about the Eurasian Economic Union, and how do they assess the potential for economic rapprochement between the CIS countries for the next five years? The paper provides the answers to these questions using the data of the annual Integration Barometer conducted by the Eurasian Development Bank in cooperation with the Eurasian Monitor (2012-2017 waves). The results show that the attitudes vary substantially depending on the economic and political situation in the world. At the same time, there has been a drop in youth interest in Russia and an increase in their preferences for Germany, the U.S., Turkey and China, whether it be an internship, courses or a job. However, young people from the Central Asia view positively the Eurasian Economic Union and appreciate the integration with the post-Soviet states. On the contrary, young Moldovans and Armenians increasingly turn their eyes toward Western countries and are less likely to cooperate with Russia in social, cultural and economic spheres.
One erroneous attribution of Defence of Eunuchs
Authors: Todorović Darko 
Published: March 2019

Abstract
The paper traces a three-century-long tradition of a mistaken attribution of the Defence of Eunuchs by Theophylact of Ohrid. Since Peter Lambeck, chief librarian of the Hofbibliothek in Vienna, identified in 1671 the author of the treatise as Theodore Pedagogue, a poorly known tutor to the emperor Constantine VII Porphyrogenitus, the incorrect attribution was readily adopted and further disseminated by a series of scholars of the next generations. Although the issue of the authorship was successfully resolved as early as 1768 by Angelo Bandini, head of the Laurentian Library in Florence, the information remained unfamiliar to writers of the following centuries, leaving the entrenched error to persist until the late 1970s, on the eve of the first critical editions of the Defence (Gautier 1980, Spadaro 1981). The article follows the tangled history of the erroneous attribution, attempting to establish a kind of stemmatic regularity between several branches of the abortive tradition.
Degrees of the approximations by some special matrix means of conjugate Fourier series
Authors: Kranz Radosława , Rzepka Aleksandra , Sylwestrzak-Maślanka Ewa 
Published: March 2019

AbstractIn this paper we will present the pointwise and normwise estimations of the deviations considered by W. Łenski, B. Szal, [Acta Comment. Univ. Tartu. Math., 2009, 13, 11-24] and S. Saini, U. Singh, [Boll. Unione Mat. Ital., 2016, 9, 495-504] under general assumptions on the class considered sequences defining the method of the summability. We show that the obtained estimations are the best possible for some subclasses of Lpby constructing the suitable type of functions.
Assessment of compliance level of ICH guidelines for organic volatile impurities in common ayurvedic hepatic formulations
Authors: Maithani M. , Raturi R. , Gupta V. , Bansal P. 
Published: March 2019

Abstract

Background
Herbal medicines have been used in the treatment of liver diseases for a long time. In recent years, the use of herbal medicines for protection from other strong antibiotics as well as drugs that can damage the liver during their metabolism in liver and for treatment of liver diseases has increased all over the world. It is important to mention that a number of organic solvents are used at different stages of extraction/formulation development for these traditional preparations in industries/pharmacies. In addition, some of these solvents possess established carcinogenic properties and may enter the formulation as residual solvents. Hence as per ICH guidelines it is mandatory to keep the level of these solvents up to permissible limits. There has been a lot of hue and cry that ayurvedic formulations available in the market are not properly standardized for their quality due to lack of stringent regulations and standards from regulatory authorities. Therefore the aim of present work was to assess the compliance of ICH guidelines for level of organic volatile impurities in common marketed ayurvedic hepatic formulations.

Methods
In this study, 25 ayurvedic herbal formulations available as OTC product have been assessed for presence of residual solvents using gas chromatography with flame ionization detector.

Results
This study on 25 fast moving hepatic formulations in the market reflects that no residual solvents were detected in any of the formulations however if present were within prescribed permissible limits of ICH guidelines. The data was also subjected to statistical analysis (F-test and t-test at 95% confidence level).

Conclusions
Results indicate the safety of these hepatic formulations with respect to residual solvents. In addition presents a simple, linear, specific, accurate, precise and rugged gas chromatographic method for estimation of residual solvents.
Characterizations of the Solution Sets of Generalized Convex Fuzzy Optimization Problem
Authors: Chen Wang , Zhou Zhiang 
Published: March 2019

AbstractThis paper provides some new characterizations of the solution sets for non-differentiable generalized convex fuzzy optimization problem. Firstly, we introduce some new generalized convex fuzzy functions and discuss the relationships among them. Secondly, some properties of these new generalized convex fuzzy functions are given. Finally, as applications, some characterizations of the solution sets for non-differentiable generalized convex fuzzy optimization problem are obtained.