bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcome of labor with epidural analgesia in primigravida at term
Authors: Mehra Reeti , Baweja Namrata , Mitra Sukanya 
Published: February 2019

Background: There is widespread acceptance of epidural analgesia among many physicians and patients, but disagreement remains regarding the effect of intrapartum epidural analgesia on the subsequent progress of labour and the mode of delivery. This study was designed to look into the effects of labour analgesia on maternal and fetal outcomes and compare the same with that of opioid analgesics.Methods: It was a prospective randomized controlled trial on 100 patients where parturients were randomly allocated to either group 1-( n=50 patients) who received epidural analgesia or group 2 (n=50) who received intramuscular tramadol. The two groups were compared for duration of labour, analgesic efficacy as assessed by using Visual Analogue Scale (VAS) ,type of delivery – normal/instrumental/operative interventions ,1 and 5 min neonatal Apgar score and neonatal breast-feeding behavior between the two groups.Results: Epidural analgesic gave better pain relief than tramadol which was found to be stastically significant (p<0.001) on comparing the VAS scores and also gave statistically significantly higher satisfaction levels. Duration of both first and second stage of labor was comparable between the two groups and instrumental deliveries and caesarean sections were also comparable to that in tramadol group. There was a trend of higher neonatal Apgar scores in the epidural group than in the tramadol group.Conclusions: Use of effective analgesia in labour, does not prolong second stage of labor and may facilitate good labour outcome both for the mother and the fetus and should be offered to patients in tertiary setups and equipped centres when the patients are apprehensive about labor pains.
Progress in plant genome sequencing: research directions
Authors: Bragina M. K. , Afonnikov D. A. , Salina E. A. 
Published: February 2019

Since the first plant genome of Arabidopsis thaliana has been sequenced and published, genome sequencing technologies have undergone significant changes. New algorithms, sequencing technologies and bioinformatic approaches were adopted to obtain genome, transcriptome and exome sequences for model and crop species, which have permitted deep inferences into plant biology. As a result of an improved genome assembly and analysis methods, genome sequencing costs plummeted and the number of high-quality plant genome sequences is constantly growing. Consequently, more than 300 plant genome sequences have been published over the past twenty years. Although many of the published genomes are considered incomplete, they proved to be a valuable tool for identifying genes involved in the formation of economically valuable plant traits, for marker-assisted and genomic selection and for comparative analysis of plant genomes in order to determine the basic patterns of origin of various plant species. Since a high coverage and resolution of a genome sequence is not enough to detect all changes in complex samples, targeted sequencing, which consists in the isolation and sequencing of a specific region of the genome, has begun to develop. Targeted sequencing has a higher detection power (the ability to identify new differences/variants) and resolution (up to one basis). In addition, exome sequencing (the method of sequencing only protein-coding genes regions) is actively developed, which allows for the sequencing of non-expressed alleles and genes that cannot be found with RNA-seq. In this review, an analysis of sequencing technologies development and the construction of “reference” genomes of plants is performed. A comparison of the methods of targeted sequencing based on the use of the reference DNA sequence is accomplished.
Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis
Authors: Illarionova N. B. , Petrovski D. V. , Razumov I. A. , Zavyalov E. L. 
Published: February 2019

Gliomas are the most common type of malignant brain tumors. Standard treatment of gliomas consists of surgical excision of the tumor with subsequent chemotherapy and radiotherapy. Tumor cells are characterized by rapid division with an increased uptake of glucose and its catabolism during glycolysis. To maintain rapid division, the level of glycolysis of the tumor cell is significantly increased, compared with normal cells. It is known that some nanoparticles (NP) have the property of accumulating in tumors. In particular, NPs of manganese oxide can penetrate into the brain and, with considerable accumulation, cause toxic effects. These facts served as a prerequisite for studying the effects of manganese oxide NPs on the viability of glioma cells. The purpose of this work was to study the effects of manganese oxide NPs, as well as their combination with gamma irradiation on the glycolysis of glioma cells. The cells were irradiated using the research radiobiological gamma-installation IGUR-1 based on 137Cs. The level of cell glycolysis was determined using the standard glycolytic stress test on a Seahorse XFp platform. Cell viability was determined using the ViaCount reagent staining of living and dead cells. Their count was performed using flow cytometry. We showed that the glycolysis of U-87 MG glioma cells was significantly reduced when incubated for 48 hours with manganese oxide NPs. Irradiation in combination with NPs or alone did not have significant effects on glycolysis of gliomas. Glioma incubation with manganese oxide NPs for 72 hours led to a significant reduction in cell viability. This study may be useful for the development of new therapies and diagnosis of gliomas.
Microspore embryogenesis in vitro: the role of stresses
Authors: Djatchouk T. I. , Khomyakova O. V. , Akinina V. N. , Kibkalo I. A. , Pominov A. V. 
Published: February 2019

Gametic embryogenesis is one form of totipotency of plant cells, in which either male or female gametes are induced to form embryoids (sporophytes). Regeneration of haploid plants from embryoids and subsequent chromosome duplication result in doubled haploids and DH-lines. The production of haploids and doubled haploids (DHs) through gametic embryogenesis allows a single-stage development of complete homozygous lines from heterozygous plants. The development of effective haploid protocols to produce homozygous plants has a significant impact on plant breeding, shorting the time and costs required to establish new cultivars. There are several available methods to obtain haploids and DHs-lines, of which anther or isolated microspore culture in vitro are the most effective. Microspore embryogenesis is more commonly applied. This is in part because more male gametophytes are contained in a single anther compared to the single female gametophyte per embryo sac. Microspore embryogenesis is regarded as one of the most striking examples of plant cell totipotency. The switch of cultured microspores from gametophytic to sporophytic mode of development has been induced by stress treatments of various kinds applied to donor plants, inflorescences, buds, anthers or isolated microspores both in vivo and in vitro. Physical or chemical pretreatments (cold and heat shock, sugar starvation, colchicine, n-butanol, gametocydes) act as a trigger for inducing the sporophytic pathway, preventing the gametophytic pathway development of microspore. The recent investigations have revealed that cold pretreatment during microspore reprogramming acts rather as an anti-stress factor alleviating the real stress caused by nutrient starvation of anthers or microspores isolated from donor plants. Under stress pretreatment a vacuolated and polarized microspore transformed into a depolarized and dedifferentiated cell, which is an obligatory condition for reprogramming their development. We summarize data concerning the role of various stresses in the induction of microspore embryogenesis and possible mechanisms of their action at cellular and molecular levels. Identification of new stresses allows creating efficient protocols of doubled haploid production for end-user application in the breeding of many important crops.
Challenges of the differential diagnosis between the subtypes of the junctional epidermolysis bullosa: presentation of two clinical cases
Authors: Kotalevskaya Yu. Yu. , Marycheva N. M. 
Published: February 2019

Background: Epidermolysis bullosa (EB) is a rare hereditary skin disease. It is subdivided into EB simplex (EBS), junctional EB (JEB), dystrophic EB (DEB) and Kindler syndrome. JEB is diagnosed in 2 per 1,000,000 of the population. There are few descriptions of clinical JEB cases in the literature. Clinical diagnosis of JEB and its subtypes is a challenge, especially in the early age. The paper presents 2 clinical cases of JEB in patients of the West Slavonic origin. Clinical case No. 1 was a girl of Ukrainian ethnicity, with confirmed definitive diagnosis of severe generalized JEB. Molecular genetic tests identified mutations of the LAMA3 gene that had not been described previously. The patient died at the age of 24 months from acute respiratory failure. When the patient was alive, her EB type and subtype was not possible to identify, because she had a combination of clinical manifestations typical for various JEB subtypes. Despite such symptoms as hoarse voice, stenoses, granulation tissue of typical location, laryngeal granulations, the girl was steadily gaining weight, with some periods of relative stabilization of the skin disease; she also had longer life longevity than was common for patients with severe generalized JEB. All this made a precise diagnosis difficult. Clinical case No. 2: an ethnic Russian boy with non-classified JEB. Molecular genetic testing helped to identify a homozygote mutation in the LAMA3 gene that had not been previously described; reliable determination of the subtype was not possible. The patient had mixed clinical manifestation similar both to generalized severe JEB and to laryngo-onycho-cutaneous (LOC) syndrome. During his lifetime, the patient was clinically diagnosed with Hallopeau acrodermatitis and LOC syndrome. The differential diagnostic problems were associated with the presence of signs not typical for each of the subtypes. Significant life longevity of the proband is not characteristic for severe generalized JEB (at the time of the publication the patient is 13 years old), whereas for LOC syndrome the absence of eye involvement is not typical, as well as severe laryngeal involvement at adolescence.Conclusion: Detailed descriptions of phenotype of JEB subtypes including rare and minimal clinical signs can be useful to study the clinical manifestations and natural course of the disease, including its differential diagnosis.
Değişik Zamanlarda Hasat Edilen Farklı Tipteki Şeker Pancarı (Beta vulgaris var. saccharifera L.) Çeşitlerinin Verim ve Bazı Kalite Özelliklerinin Belirlenmesi
Authors: Tosun Bekir , Karadoğan Tahsin , Şanlı Arif 
Published: February 2019

Isparta
ekolojik koşullarında 2012 ve 2013 yıllarında yürütülen bu araştırmada farklı
zamanlarda hasat edilen (çıkıştan 140, 155, 170, 185 ve 200 gün sonra) değişik
tiplerdeki şeker pancarı çeşitlerinin (Begonia, Aranka ve Esperanza (N tipi),
Sandrina ve Esperia (NZ tipi), Felicita ve Agnessa (Z tipi) verim ve bazı
kalite özellikleri incelenmiştir. En yüksek kök gövde ve biyolojik verim erken
sökümde Aranka, geç sökümde ise Esperia çeşitlerinden elde edilmiştir. En düşük
α-amino
azot miktarı erken sökümde Aranka (5.68 mmol/100g), geç sökümde ise Esperia
(2.05 mmol/100g) ve Esperanza (2.11 mmol/100g) çeşitlerinde belirlenmiştir. Erken
ve geç sökümlerde Z tipi olan Felicita ve Agnessa çeşitleri en yüksek polar
şeker oranına sahip olmuştur. En yüksek
polar şeker verimi erken sökümde Aranka (1490 kg/da), geç sökümde ise Esperia
(1867 kg/da) ve Sandrina (1860 kg/da) çeşitlerinden elde edilmiştir. Çalışmada,
Isparta koşullarında yüksek verim ve teknolojik olgunluk bakımından Aranka ve
Begonia çeşitlerinin 200 gün, diğer çeşitlerin ise en az 185 günlük vejetasyon
dönemini tamamlaması gerektiği sonucuna varılmıştır.
Pengaruh Konsentrasi Fosfat Terhadap Perbandingan Ca/P Hidroksiapatit dari Limbah Gipsum Industri Keramik
Authors: Kurniawan Asep Mufti , Hartini Sri , Cahyanti Margareta Novian 
Published: February 2019

Gypsum waste has the potential to be made into hydroxyapatite (HAp). In this study synthesis of HAp with hydrothermal method using microwave at heating temperature of 100 ºC was carried out. The purpose of this study was to obtain hydroxyapatite bioceramic compounds from gypsum waste, determine the ratio of Ca/P in obtained HAp and characterizing the obtained HAp. HAp characterization has been carried out using FTIR and XRD. The result showed that 2,5 grams of gypsum powder produced HAp powder yield of 65,61% to 84,43%. Calcium levels are 0,02440 % to 0,03466 %, while phosphate levels are 0,038 % to 0,055 %. The Ca/P ratio are 0,28 to 0,39. FTIR HAp characterization results indicate the presence of phosphate groups (PO43-), hydroxyl groups (OH-), and Ca-O groups. But based on XRD analysis, The HAp obtained is not a standard HAp.
Diagnosing Heart Disease using Wavelet Transformation and Adaptive Neuro Fuzzy Inference System (ANFIS) Based on Electrocardiagram (ECG)
Authors: Puspita Indah , Abadi Agus Maman 
Published: February 2019

Heart disease is the leading cause of death in the world. Heart disease is called the silent killer, because it often occcurs suddenly. Therefore, periodic cardiac examination is very necessary to reduce cases of death from heart disease.Heart disease can be known through electrocardiogram (ECG) examination. This study aims to explain the process of diagnosing heart disease through ECG using wavelet transformation and Adaptive Neuro Fuzzy Inference System (ANFIS).The process of diagnosing heart disease begins with cutting ECG signal consisting of 9-11 waves into one ECG wave, then decomposition and extraction are performed using wavelet transformation to obtain 6 parameters. The parameters will be used as input in ANFIS model. Data obtained from ECG extraction are divided into 70% training data and 30% testing data The output from the ANFIS model is a diagnosis of heart diseases, such as left bundle branch block (LBBB), right bundle branch block (RBBB), and normal. ANFIS learning is divided into 6 stages, namely clustering data with Fuzzy C-Means method, computing the degree of membership of each data, determining fixed neurons, looking for normalized firing strength, calculating the consequent parameter values, and determining network output.The results of the study obtained the best ANFIS model with 10 clusters. The level of accuracy, specificity, and sensitivity for training data is 100%, 100%, and 100%, respectively and for the testing data, the level of accuracy, specificity, and sensitivity is 100%, 100%, and 100%, respectively.
PENGARUH GAYA KEPEMIMPINAN TRANSAKSIONAL DAN TRANSFORMASIONAL, MOTIVASI SERTA KOMPETENSI TERHADAP KINERJA KARYAWAN PT. SANJAYATAMA LESTARI
Authors: Oktora Jublina , Rizan Mohamad , Situngkir Sihol 
Published: February 2019

Abstrak: Dalam penelitian ini tujuannya adalah untuk melakukan pengujian secara sistematis Pengaruh Gaya Kepemimpinan Transaksional Dan Transformasional, Motivasi Serta Kompetensi Terhadap Kinerja Karyawan PT. Sanjayatama Lestari. Sampel yang digunakan adalah karyawan PT. Sanjayatama Lestari dengan jumlah responden sebanyak 95 orang. Analisis yang digunakan adalah regresi linear berganda. Penelitian ini menguji 5 variabel yaitu Gaya Kepemimpinan Transaksional (X1), Gaya Kepemimpinan Transformasional (X2), Motivasi (X3) dan Kompetensi (X4) sebagai variabel bebas dan kinerja (Y) sebagai variabel terikat. Pengujian secara parsial menunjukkan bahwa gaya kepemimpinan transaksional berpengaruh sebesar 3,876 (positif dan signifikan) terhadap kinerja. Gaya kepemimpinan transformasional juga berpengaruh secara signifikan sebesar 3,128. Selanjutnya, motivasi juga mempunyai pengaruh secara signifikan sebesar 4,210 dan yang terakhir yaitu kompetensi berpengaruh secara signifikan sebesar 6,283.

Kata Kunci: Gaya kepemimpinan transaksional, gaya kepemimpinan transformasional, motivasi, kompetensi, kinerja dan regresi linear berganda.
PENGARUH FAKTOR EKONOMI MAKRO, STRATEGI PERLUASAN MARKET SHARE DAN KARAKTERISTIK BANK TERHADAP PROFITABILITAS PT. BANK PEMBANGUNAN DAERAH JAWA BARAT DAN BANTEN, TBK
Authors: Andriansyah Novi , Rizan Mohamad , Wiralaga Harya Kuncara 
Published: February 2019

Abstrak : Penelitian ini dilakukan untuk menguji pengaruh faktor ekonomi makro yang diproksi dengan tingkat inflasi dan pertumbuhan GDP, strategi perluasan market share yang diukur dengan penambahan jaringan kantor untuk meningkatkan penghimpunan Dana Pihak Ketiga (DPK) dan penyaluran kredit serta karakteristik bank yang diukur dengan LDR, CAR, NPL, BOPO, SIZE terhadap Profitabilitas yang diproksi dengan Return On Asset (ROA) Bank BJB. Data yang digunakan dalam penelitian ini diperoleh dari Laporan Keuangan publikasi Bank BJB selama periode triwulan I tahun 2005 sampai dengan triwulan IV tahun 2013, serta laporan publikasi Statistik Perbankan Indonesia melalui website. Teknik analisis data yang digunakan dalam penelitian ini adalah analisis regresi linier berganda dimana sebelumnya data telah diuji dengan pengujian asumsi klasik yang meliputi uji normalitas, uji heteroskedastisitas, uji autokorelasi dan uji multikolinieritas. Selama periode penelitian menunjukan bahwa data penelitian berdistribusi normal. Kemudian berdasarkan hasil uji normalitas, uji heteroskedastisitas, uji autokorelasi dan uji multikolinieritas tidak ditemukan variabel yang menyimpang dari asumsi klasik. Hal ini menunjukkan bahwa data yang tersedia telah memenuhi syarat untuk menggunakan model persamaan regresi linier berganda. Hasil penelitian ini menunjukkan bahwa variabel tingkat inflasi dan pertumbuhan GDP tidak menunjukkan pengaruh signifikan terhadap profitabilitas (ROA) Bank BJB. Begitu juga dengan variabel market share, LDR, CAR, NPL, maupun Size tidak berpengaruh signifikan terhadap profitabilitas (ROA) Bank BJB. Sedangkan variabel BOPO berpengaruh negatif signifikan terhadap profitabilitas (ROA) Bank BJB. Nilai koefisien determinasi (R2 adjusted) sebesar 0.6798 artinya bahwa variasi variabel-variabel eksogen/ independen mampu menjelaskan profitabilitas (ROA) sebesar 67.98 persen, sisanya oleh variabel lain di luar model.
Kata kunci : Faktor Ekonomi Makro, Market Share, Karakteristik Bank, Profitabilitas, Regresi Linier Berganda.
DEFANSİF TIP UYGULAMALARINA YÖNELİK BİR DERLEME
Authors: YEŞİLTAŞ Aysun , ERDEM Ramazan 
Published: February 2019

Defansif uygulamalar, hekimlerin kendilerini korumak için gösterdikleri kaçınma davranışlarıdır. Bu uygulamaların dünyada olduğu gibi Türkiye’de de hekimler tarafından yaygın olarak uygulandığı görülmektedir Bu çalışmada defansif tıp uygulamalarının nedenleri ve yaygınlığına ilişkin değerlendirme yapmak amaçlanmıştır. Defansif uygulamalar kaçınma davranışı olarak değerlendirilen negatif defansif tıp ve daha çok girişime yönlenilen pozitif defansif tıp olma üzere iki başlık altında toplanmaktadır. Her iki uygulamada da hekimleri defansif tıbba yönlendiren nedenler bulunmaktadır. Bunlar hasta-hekim iletişimi, medyanın etkisi, şiddet, hekimlerin iş yükünün artması, malpraktis davaları, sağlık sistemi, mesleki deneyimin az olması, şikâyet edilme korkusu olarak sıralanmaktır. Sonuç olarak defansif uygulamalar, hekimler ve hastalar açısından olumsuz durumlara sebep olmaktadır. Zira sağlık hizmetlerinin etkili sunumunun sağlanması için hekimleri bu uygulamalara yönelten sebeplerin irdelenmesi, sorunların çözümlenmesi gerekmektedir. Dolayısıyla defansif tıbbı önlemek için başta yöneticiler olmak üzere sağlık çalışanları ile birlikte çözümler aranmalıdır.
DEFANSİF TIBBIN NEDENLERİNİN HEKİM GÖZÜYLE DEĞERLENDİRİLMESİ
Authors: TORAMAN Aynur , ÇARIKÇI İlker Hüseyin 
Published: February 2019

Son zamanlarda sağlık sektöründe karşımızı çıkan en önemli problemlerden biri olan defansif tıp, hekimlerin malpraktis davalarından kendilerini korumak ve riskli işlemlerden kaçınmak olarak sergilemiş olduğu davranışları ifade etmektedir. Defansif tıp uygulamaları, hekimleri, hasta ve yakınlarını olumsuz etkilemekle kalmamakta aynı zamanda sistemleri de olumsuz yönde etkilemektedir. Bu bağlamda araştırmanın amacı doğrultusunda hekimleri defansif tıbba iten nedenler hakkında bilgi edinmek üzere 23 öğretim üyesi hekim ile görüşülmüştür. Nitel bir araştırma olan bu çalışma fenomenoloji deseni ile dizayn edilmiş ve amaçlı örneklem yönteminden ölçüt örnekleme tipi kullanılarak tasarlanmıştır. Araştırmada elde edilen veriler betimsel ve içerik analiz yöntemleriyle analiz edilmiştir. Verilerin analizinde, nitel veri analiz programı NVivo 11 Pro kullanılmıştır. Hekimlerin malpraktis davaları başta olmak üzere hasta şikayetleri, sağlık politikaları, performans sistemi ve hekime karşı şiddet gibi birçok sebepten dolayı defans yaptıkları belirlenmiştir.
The efficacy and safety of Sipjeondaebo-tang in Korean patients with cold hypersensitivity in the hands and feet: A protocol for a pilot, randomized, double-blind, placebo-controlled, parallel-group clinical trial
Authors: Ko Youme , Sun Seung-Ho , Han In-Sik , Go Ho-Yeon , Kim Tae-Hun , Lee Jin-Moo , Jang Jun-Bok , Park Kyoung Sun , Song Yun-Kyung , Lee Kyou-Young , Jeon Chan-Yong , Ko Seong-Gyu 
Published: February 2019

Abstract
Background

Cold hypersensitivity in the hands and feet (CHHF) is frequent in Asian countries including Korea. The quality of life can be degraded by the symptoms of CHHF. In particular, gynecological disorders such as menstrual pain, infertility, leucorrhea, and irregular bleeding may be related to CHHF. Sipjeondaebo-tang(SDT) is widely used in the treatment of various diseases including CHHF by balancing Yin and Yang, restoring the deterioration of physiological function, and improving immunity. However, the efficacy of SDT in the treatment of CHHF has not been assessed in clinical trials. Therefore, we aimed to investigate the feasibility of a full randomized clinical trial of SDT for the treatment of CHHF in Korean women through this trial.

Methods

This study will be a pilot, randomized, double-blind, two-arm, placebo-controlled, parallel-group, multicenter clinical trial. Women aged 19-59 years who present with CHHF will be recruited from five university hospitals. A total of 60 subjects will be randomly assigned to a treatment group (SDT) or a placebo group at a 1:1 ratio. The subjects will receive 3 g of either SDT or placebo three times daily for 8 weeks. The primary outcome measures will be the Visual Analogue Scale scores of CHHF. The secondary outcome measures will be changes in body temperature in both the hands and the feet as measured by using a thermometer and the Korean version of the World Health Organization Quality of Life Scale Abbreviated Version.

Discussion

This will be the first trial to investigate the efficacy and safety of SDT in the treatment of CHHF. This study will provide basic clinical information regarding Korean herbal medicine treatment of CHHF and a clinical basis for designing a full randomized clinical trial.