bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomics analysis of round and wrinkled pea (Pisum sativum L.) seeds during different development periods
Authors: Daba Sintayehu D. , Panda Punyatoya , Aryal Uma K. , Kiszonas Alecia M. , Finnie Sean M. , McGee Rebecca J. 
Published: October 2024

AbstractSeed development is complex, influenced by genetic and environmental factors. Understanding proteome profiles at different seed developmental stages is key to improving seed composition and quality. We used label‐free quantitative proteomics to analyze round and wrinkled pea seeds at five growth stages: 4, 7, 12, 15, and days after anthesis (DAA), and at maturity. Wrinkled peas had lower starch content (30%) compared to round peas (47%–55%). Proteomic analysis identified 3659 protein groups, with 21%–24% shared across growth stages. More proteins were identified during early seed development than at maturity. Statistical analysis found 735 significantly different proteins between wrinkled and round seeds, regardless of the growth stage. The detected proteins were categorized into 31 functional classes, including metabolic enzymes, proteins involved in protein biosynthesis and homeostasis, carbohydrate metabolism, and cell division. Cell division‐related proteins were more abundant in early stages, while storage proteins were more abundant later in seed development. Wrinkled seeds had lower levels of the starch‐branching enzyme (SBEI), which is essential for amylopectin biosynthesis. Seed storage proteins like legumin and albumin (PA2) were more abundant in round peas, whereas vicilin was more prevalent in wrinkled peas. This study enhances our understanding of seed development in round and wrinkled peas.The study highlighted the seed growth patterns and protein profiles in round and wrinkled peas during seed development. It showed how protein accumulation changed, particularly focusing on proteins implicated in cell division, seed reserve metabolism, as well as storage proteins and protease inhibitors. These findings underscore the crucial role of these proteins in seed development. By linking the proteins identified to Cameor‐based pea reference genome, our research can open avenues for deeper investigations into individual proteins, facilitate their practical application in crop improvement, and advance our knowledge of seed development.
Thiol‐Acrylate Gel Systems For Frontal Polymerization
Authors: Adrewie Dominic , Rocha Monica , Fuller Mason , Pojman John A. 
Published: October 2024

ABSTRACTA trithiol‐triacrylate gel system for frontal polymerization was explored to establish the gelation time, shelf life, and frontal kinetics. The free‐standing gels were created by triethylamine‐catalyzed Michael addition of trimethylolpropane tris(3‐mercaptopropionate) to trimethylolpropane triacrylate such that sufficient acrylate functional groups were left unreacted to allow free‐radical frontal polymerization with the initiator 1,1‐bis(tert‐butylperoxy)‐3,3,5‐trimethylcyclohexane (Luperox 231). Systems with gelation times between 30 and 60 min that support frontal polymerization after up to 28 days of storage were achieved. The front velocity was found to depend on the 1,1‐bis(tert‐butylperoxy)‐3,3,5‐trimethylcyclohexane concentration. However, the amount of triethylamine, which was used to catalyze gel formation, did not significantly affect front velocity. The gel diameter and addition of milled carbon fiber (Zoltek px35) affected the front velocity. Cracks during frontal polymerization were reduced when Zoltek px35 was added to the formulation, which also increased the mechanical strength. Complex geometries of free‐standing gels were successfully polymerized. This system is potentially useful in situations where molding and reshaping gels are required prior to frontal polymerization, as well as enabling the ability to examine how mechanical forces like stretching and compression can affect front kinetics.
Understanding LC/MS‐Based Metabolomics: A Detailed Reference for Natural Product Analysis
Authors: Sarkar Jyotirmay , Singh Rajveer , Chandel Shivani 
Published: October 2024

ABSTRACTLiquid chromatography, when used in conjunction with mass spectrometry (LC/MS), is a powerful tool for conducting accurate and reproducible investigations of numerous metabolites in natural products (NPs). LC/MS has gained prominence in metabolomic research due to its high throughput, the availability of multiple ionization techniques and its ability to provide comprehensive metabolite coverage. This unique method can significantly influence various scientific domains. This review offers a comprehensive overview of the current state of LC/MS‐based metabolomics in the investigation of NPs. This review provides a thorough overview of the state of the art in LC/MS‐based metabolomics for the investigation of NPs. It covers the principles of LC/MS, various aspects of LC/MS‐based metabolomics such as sample preparation, LC modes, method development, ionization techniques and data pre‐processing. Moreover, it presents the applications of LC/MS‐based metabolomics in numerous fields of NPs research such as including biomarker discovery, the agricultural research, food analysis, the study of marine NPs and microbiological research. Additionally, this review discusses the challenges and limitations of LC/MS‐based metabolomics, as well as emerging trends and developments in this field.
Evaporation, spreading, and possible uptake of droplets on sorghum (Sorghum bicolor) and cowpea (Vigna unguiculata) leaves using an imaging‐based technology
Authors: Makhnenko Iaroslav , Hoerning Cody , Sawall Dustyn D , Fredericks Steven A , Alonzi Elizabeth R , Dutcher Cari S 
Published: October 2024

AbstractBACKGROUNDSprayed agrochemical droplets have a dynamic evolution on the leaf surface, undergoing changes in shape and volume due to spreading, evaporation, and adsorption. To better understand these processes, an accessible imaging‐based experimental methodology is presented to precisely measure droplet spreading, evaporation, and potential uptake by a leaf within a controlled relative humidity environment. Laboratory experiments were conducted to determine the effect of hydrocarbon surfactants, accelerators (light mineral oil), and humectants (high fructose corn syrup) on droplet spread, evaporation, and potential uptake when applied to sorghum (Sorghum bicolor ‘Tricker’) and cowpea (Vigna unguiculata) leaves.RESULTSExperiments on cowpea leaves showed uniform spreading and no change in evaporation compared to the predicted rate. In contrast, on sorghum leaves, results suggest that the volume loss rate exceeds the predicted evaporation rate (up to 23%), indicating a potential uptake by the leaves. Some accelerated dynamics on sorghum can be attributed to the lateral spreading observed on hairy leaves along the veins, increasing the contact area by an average of 65%. However, samples containing light mineral oil, typically considered an accelerant to aid in uptake, demonstrated the highest rate but exhibited minimal spreading.CONCLUSIONSThe study demonstrates how droplet composition affects droplet dynamics on waxy and hairy leaves by using an imaging‐based methodology to measure evaporation rates, volume loss, contact angle, wetted area, and spreading behavior. The findings highlight some of the complex coupling between the crop protection product composition and droplet life cycle on a leaf. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Ectopic expression of the mitochondrial protein COXFA4L3 in human sperm acrosome and its potential application in the selection of male infertility treatments
Authors: Fujisawa Yusuke , Kikuchi Sayaka , Kuba Fujino , Oishi Kosei , Murayama Soushi , Sugiyama Tomoya , Tokito Reiji , Ueno Hiroe , Kashiwabara Shin‐ichi , Yumura Yasushi , Kurihara Yasuyuki 
Published: October 2024

AbstractPurposeSpermatogenesis requires a large amount of energy, which is primarily produced by the mitochondrial electron transfer chain. Mitochondrial dysfunction affects male infertility, suggesting a relationship between the electron transfer chain and male infertility. COXFA4L3 (C15ORF48) is an emerging subunit protein of cytochrome oxidase specifically expressed in germ cells during spermatogenesis, and it may be involved in male infertility. Therefore, to investigate whether COXFA4L3 could be a marker of mitochondrial dysfunction in the sperm, this study examined the protein expression and localization profile of COXFA4L3 in the sperm of male patients with infertility.MethodsTwenty‐seven semen samples from a male infertility clinic at the Reproductive Center of Yokohama City University Medical Center were used to analyze sperm quality parameters and the expression and localization of energy production‐related proteins. These data were compared with the outcomes of infertility treatment.ResultsThe expression levels of COXFA4L3 varied significantly between samples. Furthermore, COXFA4L3 was ectopically localized to the acrosome.ConclusionsEctopic expression of COXFA4L3 and PNA‐stained acrosomes may be useful parameters for fertility treatment selection. Assessing the acrosomal localization of COXFA4L3 will expedite pregnancy treatment planning.
H∞$$ {\boldsymbol{H}}_{\mathbf{\infty}} $$ Control With Event‐Triggered Mechanism for T‐S Fuzzy System Under Multiple Cyber‐Attacks
Authors: Tan Cheng , Gao Chengzhen , Guo Ge 
Published: October 2024

ABSTRACTThe problem of event‐triggered control for networked Takagi–Sugeno (T‐S) fuzzy system under aperiodic DoS attacks and deception attacks is investigated. First, an event generator is introduced in the Sensor‐to‐Controller channel to determine the transmission of data. At the same time, the Sensor‐to‐Controller channel is assumed to be subjected to deception attacks that are randomly distributed but not Bernoulli distributed. Next, the impact of aperiodic DoS attacks on the Controller‐to‐Actuator channel is further considered, and the DoS attack behavior is described in terms of attack period and frequency. The article designs an adaptive resilience event‐triggered mechanism (ARETM), which is aimed at circumventing the ineffective data updating in the “active” phase of the DoS attacks, thereby realizing the effective saving of communication resources and mitigating the adverse effects of DoS attacks. Then, the switched fuzzy system is established to cope with the different states of the DoS attackers. Using the piecewise Lyapunov function, a design method for the controller gains and the ARETM matrix is obtained, which allows the system to be stabilizable and obtain performance under the control action. Finally, the effectiveness of the ARETM‐based control strategy is confirmed by simulation experiment.
Dynamic Self‐Reconstruction Heterostructures: Boosting the High‐Stability for Hydrogen Evolution of NiMo Alloys in Acidic Environments
Authors: Li Jieyao , Hu Wenhui , Sun Lingna , Zhang Lei , Zhang Qianling , Ren Xiangzhong , Li Yongliang 
Published: October 2024

AbstractElectrocatalytic water splitting is considered one of the most promising approaches for large‐scale hydrogen production. However, designing transition metal catalysts with high durability under acidic conditions remains a significant challenge. The durability of the catalyst is closely related to the changes of the catalyst during its operation, and constructing effective surface reconstruction strategies can help address the durability issues of transition metals in acidic hydrogen evolution reactions (HER). Herein, the spontaneous formation of surface‐reconstructed heterostructures of NiMo alloys is reported during the HER in acidic media. The surface of the catalyst is characterized by the presence of Ni/Mo metal nanoparticles and NixMoyOz nanosheets, which coexist as HER proceeds. Notably, the E‐Ni90Mo10/CC After 96 h catalyst demonstrates a significantly reduced overpotential of 56.84 mV at 10 mA cm⁻2 in 0.5 m sulfuric acid, which is better than other E‐Ni90Mo10/CC counterparts. Both experimental data and theoretical calculations suggest that these spontaneously formed heterostructures are helpful for optimizing hydrogen adsorption. Furthermore, the downward shift of the d‐band center within the heterostructure (Ni90Mo10/NixMoyOz) is found to facilitate the desorption of intermediate products, thereby enhancing the overall HER activity. This work provides a new perspective for designing highly durable transition metal catalysts for acidic HER.
Chemical‐Physical Synergistic Assembly of MXene/CNT Nanocoatings in Silicone Foams for Reliable Piezoresistive Sensing in Harsh Environments
Authors: Chen Zuan‐Yu , Liu Shuai‐Chi , Wu Yu‐Xi , Wu Yu‐Yue , Peng Li‐Dong , Wang Ye‐Jun , Nie Feng , Zhao Li , Lv Pei‐Yuan , Cao Cheng‐Fei , Li Yang , Zhang Guo‐Dong , Bae Joonho , Cao Kun , Tang Long‐Cheng 
Published: October 2024

AbstractOwing to their high sensitivity across a wide stress range, mechanical reliability, and rapid response time, flexible polymer foam piezoresistive sensors have been extensively used in various fields. The reliable application of these sensors under harsh environments, however, is severely limited by structural devastation and poor interfacial bonding between polymers and conductive nanoparticles. To address the above issues, robust MXene/CNT nanocoatings on the foam surface, where the chemical assembly of MXene nanosheets and the physical anchoring of CNTs lead to strong interfacial bonding, are designed and described, which endows foams with structural reliability and unexpected multi‐functionalities without compromising their instinct properties. The optimized foam nanocomposites thus maintain outstanding wide‐temperature flexibility (−60–210 °C) and elasticity (≈3% residual strain after 1000 cycles). Moreover, the nanocomposites display good sensitivity at a relatively wide stress range of 0–70% and remarkable stability under acidic and alkaline settings. Furthermore, the foams with exceptional fire resistance (UL‐94 V‐0 rating) can provide stable sensing behavior (over 300 cycles) even after being exposed to flames for 5 s, making them one of the most reliable sensing materials so far. Clearly, this work widens applications of flexible piezoresistive sensors based on silicone foam nanocomposites for various harsh environments.
Boosting FRET Efficiency of Chromophores with Aggregation‐Caused Quenching by a Crystallization‐Induced Precise Co‐assembly Strategy
Authors: Zhou Qian , Zhang Xiaomin , Ning Lijian , Song Yuhui , Wang Yanli , Feng Jinkun , Sun Chun‐Lin , Li Jun , Gong Qiuyu , Zhang Qichun , Huang Yinjuan 
Published: October 2024

AbstractFörster resonance energy transfer (FRET) plays a critical role in organic optoelectronic materials. However, developing facile and effective strategies to achieve high‐efficiency energy harvesting of chromophores with aggregation‐caused quenching (ACQ) remains an appealing yet challenging task, that has not yet been explored. Herein, a subtly strategy, crystallization‐induced precise co‐assembly (CIPCA) involving a molecular “lightening agent,” to effectively improve FRET efficiency of ACQ chromophores is developed. Bis(phenylethynyl)anthracene (BPA) and bis(phenylethynyl)naphthacene (BPN) with significant ACQ effect are chosen as representative FRET donor and acceptor, respectively, and weakly‐fluorescent octafluoronaphthalene (OFN) acted as the “lightening agent.” Thanks to precise co‐assembly with OFN, the PLQY of solid BPA is enhanced by 107%, and the BPN powder can be unprecedentedly lighted. More importantly, through such powerful CIPCA, the monotonous and weak emission for BPA@BPN can be remarkably regulated to colorful and much brighter ones with FRET efficiency improvement of as high as 180–270%. An in‐depth understanding of FRET regulation is elucidated through a precise correlation of the supramolecular structures and properties. Such achievements allow to successfully fabricate distinct multi‐stimuli‐responsive fluorescent patterns and highly‐emissive colorful flowers with high flexibility. This research provides an efficient strategy to improve the FRET efficiency of ACQ pairs.
Quality by Design Approach Driven Development and Validation of Reverse Phase‐High‐Performance Liquid Chromatography Methodology for Dual Quantification of Aspirin and Rivaroxaban
Authors: Patel Mehul , Patel Hetali , Patel Rashmin , Shah Umang , Thakkar Mansi , Patel Ashish , Patel Swayamprakash , Solanki Nilay 
Published: October 2024

ABSTRACTClinical trials have shown that combining a modest dose of Rivaroxaban (RIV) with Aspirin (ASP) is more effective than ASP alone in reducing cardiovascular mortality, myocardial infarction, and stroke. A high‐performance liquid chromatography method developed using Quality by Design (QbD) effectively estimates ASP and RIV for routine pharmaceutical analysis. The Plackett‐Burman design assessed key factors including organic phase volume, mobile phase pH, flow rate, temperature, and injection volume using an Ishikawa fish‐bone diagram for risk assessment. These parameters were then optimized via Box‐Behnken design in 15 trials. The method meets the International Council For Harmonization standards with a mobile phase ratio of 45:55 (v/v) acetonitrile: buffer (pH 3, adjusted with orthophosphoric acid), a flow rate of 1 mL/min, a column temperature of 30°C, and an injection volume of 20 µL. A Phenomenex Luna C18 column (250 × 4.6 mm, 5 µm particle size) with detection at 251 nm ultra‐violet wavelength. The retention times of 4.54 min for ASP and 5.81 min for RIV were obtained. Validation confirmed linear ranges: 40–200 µg/mL for ASP and 2–10 µg/mL for RIV, with excellent recovery rates (ASP: 99.30%–101.9%; RIV: 98.52%–101.84%). Developed using QbD principles, the method proved specific, accurate, precise, sensitive, and robust for routine quality control of both compounds in pharmaceutical formulations.
Genome‐wide association study uncovers pea candidate genes and metabolic pathways involved in rust resistance
Authors: Osuna‐Caballero Salvador , Rubiales Diego , Rispail Nicolas 
Published: October 2024

AbstractPea (Pisum sativum L.) is an important temperate legume crop providing plant‐based proteins for food and feed worldwide. Pea yield can be limited by several biotic stresses, among which rust represents a major limiting factor in many temperate and subtropical regions. Some efforts have been made to assess the natural variation in pea resistance to rust, but its efficient exploitation in breeding is limited since the resistance loci identified so far are scarce and their responsible gene(s) unknown. To overcome this knowledge gap, a comprehensive genome‐wide association study (GWAS) has been performed on pea rust, caused by Uromyces pisi, to uncover genetic loci associated with resistance. Utilizing a diverse collection of 320 pea accessions, we evaluated phenotypic responses to two rust isolates using both traditional methods and advanced image‐based phenotyping. We detected 95 significant trait‐marker associations using a set of 26,045 Diversity Arrays Technology‐sequencing polymorphic markers. Our in silico analysis identified 62 candidate genes putatively involved in rust resistance, grouped into different functional categories such as gene expression regulation, vesicle trafficking, cell wall biosynthesis, and hormonal signaling. This research highlights the potential of GWAS to identify molecular markers associated with resistance and candidate genes against pea rust, offering new targets for precision breeding. By integrating our findings into current breeding programs, we can facilitate the development of pea varieties with improved resistance to rust, contributing to sustainable agricultural practices and food security. This study sets the stage for future functional genomic analyses and the application of genomic selection approaches to enhance disease resistance in peas.
Consumer Perception of Personal Care Products and Cosmetics on Health and Environmental Effects
Authors: Gamage Sachith Gihan , Mahagamage Mahagama Gedara Yohan Lasantha 
Published: October 2024

ABSTRACTPersonal care products and cosmetics have become an essential part of present society. Since the composition of these products directly correlates with public health and the environment, understanding consumer behaviors and attitudes is a significant aspect in controlling their negative impacts. The present study surveyed 300 consumers in Sri Lanka to attain insights into the selection, usage, disposal, and participants' attitudes and knowledge of these products. The majority of the respondents were female (58%) and 71% were between the ages of 18 and 65. Most of them (48%) selected products by their evaluations and 34% were highly concerned about the ingredients. Removal of applied products was mainly done by washing (97%) and in many households, generated wastewater ended up in wastewater gullies (69%). The remaining plastic packages were discarded into municipal garbage trucks (42%) or burned (31%). Alarmingly, 29% of the participants have experienced some kind of adverse reaction by using these products during their lifetime. Therefore, proper monitoring, waste management, and regulation of these products could be beneficial to control many related health and environmental consequences.
Water Quality Analysis and Risk Assessment of Potential Toxic Elements in Selected Surface Water Around Mining Sites in Abuakwa South Municipal, Ghana
Authors: Baah Douglas Siaw , Amissah Lucy , Foli Gordon , Gikunoo Emmanuel , Atambire James 
Published: October 2024

ABSTRACTSurface water quality at Abuakwa South Municipal has received threats from surrounding mining communities, which are becoming a major concern to the public. As a result, this study aimed to assess water quality by examining the concentration of lead (Pb), cadmium (Cd), and zinc (Zn) pollution in surface water. Fifteen samples were obtained from surface water using a 1.5 L plastic bottle. The mean concentrations of Pb and Cd were above WHO guidelines of 0.01 and 0.003 mg/l, respectively, except for Zn which was below the stipulated limit of 3.0 mg/l. The geochemical process model shows that Cd, Pb, and Zn are adsorbing (released from the drainage to the surrounding environment) in sites 1 and 3 whereas Pb is desorbing (added to the drainage) in site 2. A hazard quotient greater than 1 was recorded for Cd in adults through the ingestion route of exposure at all the sites. Cancer risk also shows that the inhabitants are at risk through the ingestion route of exposure. The findings of this study suggest intake of water draining within the vicinity is unsafe and poses severe health risks.