bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Low‐Cost Microfluidic Device For the On‐Line Counting of Microparticle/Bacteria
Authors: Xun Zhen‐Yu , Liu Lv , Zhang Bai‐Chuan , Fan Liang‐Liang , Zhang Lu‐Wei , Zhao Hong , Iqbal Sohail , Shakoor Rana Iqtidar , Zhao Liang 
Published: December 2024

ABSTRACTOn‐line counting of the microparticle/bacteria in the liquid medium has great potential in the food safety and biomedical fields. A new low‐cost microfluidic device is proposed for the on‐line counting of the microparticles/bacteria in the liquid medium. The gradually contracted microchannel and the viscoelastic fluid are combined to achieve the efficient elastic focusing of the particle/bacteria, which significantly improves the counting accuracy by aligning all particles/bacteria in a single position at the center of the microchannel. A simple light resistance‐based counting method is designed and integrated with the microchannel, where the low‐cost elements including the laser pointer, convex lens, diaphragm, and photodiode, are used to build the optical path of detection. The influence of the flow rate, the particle size, the property of fluid, and the channel structure on the focusing of the particle/bacteria are investigated by the experiment, and the counting ability of the integrated microfluidic device is validated by using different‐sized microparticles and the bifidobacterium. With its simple structure, low cost, easy operation, and high efficiency, this microfluidic device is suitable for the commercial applications, such as the on‐line counting of the plastic microparticle in water or the colony‐forming units (CFU) of bacteria in food.
Navigating the reality of school shootings with adolescents through middle grade novels
Authors: DeFauw Danielle L. 
Published: December 2024

AbstractPersonally and professionally, the author shares experiences with school safety and how the English Language Arts (ELA) classroom may utilize middle grade novels to address gun violence with adolescents. Highlighting five middle grade novels that address school shootings—Katherine Erskine's (2011) Mockingbird, Emily Barth Isler's (2021) Aftermath, J. S. Puller's (2022) The Lost Things Club, Jasmine Warga's (2022) The Shape of Thunder, and Erin Bow's (2023) Simon Sort of Says, this discussion provides ELA teachers and stakeholders with before, during, and after reading prompts to facilitate students' engagement with the topic through ELA tasks that support critical thinking and open dialog.
Shear Wave Optical Coherence Elastography and Structural Analysis of the Postnatal Mouse Cornea through Development
Authors: Lopez Andrew L. , Dehshiri Mohammad , Schill Alexander W. , Aglyamov Salavat , Larin Kirill 
Published: December 2024

ABSTRACTThe cornea is a transparent lens at the forefront of the eye, serving as a structural barrier that protects the eye and provides the majority of the eye's refractive power. In this study, we utilized the postnatal mouse eye to characterize corneal structure and biomechanics. Between postnatal day (PN) 6 and PN24, we observed that elastic wave speeds are highest at PN6, gradually decrease through PN24, and then start to rise in adulthood (6 months). We found that corneal thickness is uncoupled from elastic wave speed and that the content and organization of the cornea primarily influence its mechanical properties. To the best of our knowledge, this work is the first detailed assessment of the postnatal mouse cornea's structure and biomechanics and warrants further investigation into the dynamic properties of the postnatal eye.
Assessing the impact of online collaborative problem solving on a calculus class for first‐year engineering students: A communities of practice lens
Authors: Díaz Brayan , Luengo‐Aravena Daniela , Barahona Pia , Felmer Patricio 
Published: December 2024

AbstractBackgroundWe examine the efficacy of an online collaborative problem‐solving (CPS) teaching approach in academic performance and student connections with other peers, among first‐year engineering calculus students at a Latin American university. Our research uses communities of practice (CoP) to emphasize the social nature of learning and the importance of participation and interaction within a community.MethodsThe work applies a quasi‐experimental design and social network analysis (SNA). A total of 202 engineering students were instructed using CPS methodology (experimental group), while 380 students received traditional online teaching methods (control group) during one semester in the first calculus class for engineers.ResultsResults show no significant difference in the grades obtained between the experimental and control groups. However, students exposed to CPS reported a statistically significant higher passing rate, as well as larger and more significant academic and social connections. Additionally, SNA results suggest that CPS facilitated stronger peer connections and promoted a more equitable distribution of participation among students, particularly women, compared to students taught under traditional online teaching methods.ConclusionsThe study underscores the importance of fostering collaborative learning environments and highlights CPS as a strategy to enhance student performance and network formation. Findings suggest that CPS can improve academic outcomes and promote more equitable learning practices, potentially reducing dropout rates among women engineering students. These findings contribute to the ongoing efforts to address systematic biases and enhance learning experiences in engineering education.
Study on the Clinical Characteristics, Persistent Infection Ability and Viral Load of Human Papillomavirus Type 73 Single Infection
Authors: Chen Zuyi , Nie Shuangshuang , Ran Jing , Wang Ying , Li Qiongyao , Zhao Hongjuan , Chen Xiaoyan , Xiong Dake , Xiao Daimin 
Published: December 2024

ABSTRACTHuman papillomavirus (HPV) infection is a main cause of cervical cancer. Different HPV types exhibit significant differences in pathogenicity. Clinical data on HPV73 single infection is limited, this study aimed to analyze the clinical characteristics of HPV73 single infections. We collected cervical exfoliated cells to examine the infection rate and the clinical characteristics of patients with single HPV73 infection, and to investigate the association of viral load, persistent infection, and pathogenicity. The positive rate of HPV73 among women tested was approximately 0.14% (609/435 072). Among 214 HPV73 single infection cases, the number of patients with normal cytology, cervical intraepithelial neoplasia (CIN)1, CIN2, CIN3, and cervical cancer were 182, 24, 5, 1, and 1, respectively. Follow‐up data showed that 14.29% (3/21) of CIN1 patients developed to higher‐grade lesions, with 3.73% (5/134) of normal patients progressed to CIN. The viral load in the CIN group was significantly higher than that in the normal group (p = 0.001), and the viral load in the persistent infection group was significantly higher than that of the viral‐clearance group (p < 0.001). HPV73 single infection can lead to cervical cancer, with relatively low pathogenicity. And high viral load is a crucial factor in its persistent infection and pathogenicity.
Neurologic Music Therapy's Impact on Neurological Disorders
Authors: Wei Yaming , Qiao Zhen 
Published: December 2024

ABSTRACTNeurologic music therapy (NMT) represents a groundbreaking, interdisciplinary approach that combines the therapeutic properties of music with neuroscientific principles to treat a range of neurological and psychiatric conditions. This interdisciplinary approach, increasingly recognized in clinical and research settings, leverages advances in neuroimaging to explore how music affects the structure and activity of the brain. This review provides an in‐depth exploration of the multifaceted effects of NMT on brain function, highlighting its role in promoting neuroplastic changes and enhancing cognitive, emotional and motor functions in diverse patient groups. This review consolidates current knowledge on NMT and provides insights into how music affects brain structure and function and the mechanisms of action. The article then discusses the application and research results of NMT in various diseases such as stroke, Alzheimer's disease and Parkinson's disease. Its potential in personalizing therapeutic interventions and its ability to improve treatment access and effectiveness in various settings are highlighted.
Beyond One‐Size‐Fits‐All: Understanding the Impacts Of Heterogeneous Stakeholders On Firm Performance
Authors: Bischoff Lena , López Manuel Lucas , Vázquez Vicente Xosé Henrique 
Published: December 2024

ABSTRACTDrawing on stakeholder and transaction cost theory, we empirically investigate how managerial efforts to engage five different key stakeholder groups affect firm financial performance. Building upon the existing literature on stakeholder heterogeneity, we propose a framework that highlights inter‐stakeholder differences to empirically test whether the commonly used overarching stakeholder groupings are sufficient. This approach allows us to hypothesize about the financial value creation and value destruction potential of integrating each stakeholder individually. Our propositions are tested using a panel dataset consisting of 4926 multinational companies from 2005 to 2020. The results show that the integration of minority shareholders and communities negatively affects financial performance. Conversely, integrating customers and suppliers has a positive impact on financial performance in the long run, while integrating employees has a positive impact in the short run. These findings underscore the particularities of each stakeholder group, pointing to the need to consider heterogeneity in future stakeholder research.
Diagnostic value of sFlt‐1/PlGF‐1 ratio and plasma PROK1 for adverse pregnancy outcomes in women with hypertensive disease of pregnancy
Authors: Lv Ping , Lu Lin‐Fei 
Published: December 2024

AbstractHypertensive disease of pregnancy (HDP) is one of the most important causes of increased maternal mortality and perinatal complications during pregnancy. We investigated the pregnancy outcomes of 156 HDP patients (65 gestational hypertension [GH], 13 chronic hypertension [CH], 74 preeclampsia‐eclampsia [PE‐EC], and 4 superimposed on PE [CH with PE]). In patients with different types of HDP, levels of soluble fms like tyrosine kinase‐1 (sFlt‐1), placental growth factor (PlGF)‐1, and prokinin‐1 (PROK1) were measured and compared. The predictive efficacy of these indicators was evaluated using receiving operating characteristics curves and area under the curve. Results showed that the PE cohort had a higher sFlt‐1/PlGF ratio (46.12 [39.24, 68.85]) and PROK1 (498.84 [213.67, 678.30] pg/mL) level than the GH (sFlt‐1/PlGF, 32.3 [21.98, 58.00], PROK1 300.77[250.0, 345.29]pg/mL) and CH cohort (sFlt‐1/PlGF, 37.49 [32.68, 39.68], PROK1, 281.48 [229.25, 453.94]pg/mL). In the HDP cohort, 54 patients experienced adverse pregnancy events, and the sFlt‐1/PlGF ratio, PROK1, and the combined indicators (sFlt‐1/PlGF ratio and PROK1) were excellent predictors of adverse pregnancy events, especially for PE patients.
Low‐frequency noise affects development and movement patterns of a calanoid copepod
Authors: Tremblay Nelly , Juma Gabriel A. , Herstoff Emily M. , Meunier Cédric L. , Boersma Maarten 
Published: December 2024

AbstractAnthropogenic stressors are omnipresent in marine environments and interfere with organisms of all sizes, from large whales to small organisms. We investigated potential interactive multistressor effects of increased temperature with chronic low‐frequency sound on the development and movement patterns of the calanoid copepod Acartia tonsa, a model species for small marine zooplankton. Copepods were reared while exposed to chronic low‐frequency sound around 15 and 22 dB re 1 μPa2 Hz−1 above control sound pressure levels at frequencies between 110 and 120 Hz, at 21°C (increased temperature) and 18°C (control temperature). For each sound and temperature scenario, we assessed after‐incubation development rate, stage distribution, and movement patterns. We found that fewer copepods reached the developmental stages copepodites IV to VI in low‐frequency sound conditions, even though warmer conditions increased developmental rate. By using high‐speed videography in both control and low‐frequency sound conditions, we observed that copepods showed more escape behaviors (drops) and fewer feeding‐associated behaviors (helical swimming) when exposed to low‐frequency sound (~ 42 dB higher than normal at 142 Hz). Copepods reared with added low‐frequency sound showed fewer feeding‐associated behaviors and did not reverse these trends despite the absence of added sound in their feeding environment. These significant behavioral changes suggest detrimental negative, life‐long, consequences for copepods exposed to low‐frequency sound.
Differences in factors determining taxon‐based and trait‐based community structures: a field test using zooplankton
Authors: Suzuki Hiromichi , Ichiyanagi Hidetaka , Kass Jamie M. , Urabe Jotaro 
Published: December 2024

AbstractEcological community structure, which has traditionally been described in terms of taxonomic units, is driven by dispersal and environmental filters. Traits have recently been recognized as alternative units for quantifying community parameters, but they may have important differences with taxonomic units. For example, as taxon‐based community structure is determined by the local species pool, it may be more dispersal‐limited, whereas trait‐based community structure may be more regulated by environmental conditions because traits are less tied to specific habitat locations. This implies that the relative importance of these two filters may vary depending on the units describing community structure, but no study has yet quantified how the contributions of these filters can differ. In this study, we examined zooplankton assemblages in 87 artificial reservoirs throughout the Japanese archipelago to quantify the relative importance of two filters by examining the effects of spatial configuration (reflecting dispersal filters) and biotic and abiotic variables (reflecting environmental filters) for taxon‐ and trait‐based community structure. Variation in the taxon‐based community structure was explained equally well by the spatial and the environmental variables, while variation in the trait‐based community structure was explained more by environmental variables. These results support the idea that environmental filters play a more central role in determining trait‐based community structures, and show that the relative importance of spatial and environmental filters changes with the way we define community structure.
Consistent cell‐specific carbon fixation rates by small eukaryotic phytoplankton in contrasting nutrient‐limited conditions
Authors: Ong Denise Rui Ying , Gutiérrez‐Rodríguez Andrés , Safi Karl A. , Marie Dominique , Selph Karen E. , Stukel Michael R. , Décima Moira , Lopes dos Santos Adriana 
Published: December 2024

AbstractSmall phytoplankton, consisting of pico and nano size fractions, are diverse in size and taxonomy. Yet, the differences in their productivity and taxonomic diversity are poorly described. Here, we measured the cell‐specific carbon fixation rates of picocyanobacteria Synechococcus, picoeukaryote, and nanoeukaryote populations, while unveiling their taxonomic composition in oligotrophic subtropical and high‐nutrient low‐chlorophyll subantarctic waters. We coupled 24 h in situ radiolabeled 14C incubations to flow cytometry sorting and DNA metabarcoding from the same incubated samples, offering a direct account of the community associated with the carbon fixation rates measured. In both water masses, nanoeukaryotes had the highest cell‐specific carbon fixation rate, followed by picoeukaryotes and Synechococcus (2.24 ± 29.99, 2.18 ± 2.08, and 0.78 ± 0.55 fgC cell−1 h−1, respectively). The cell‐specific carbon fixation rates and growth rates of Synechococcus were threefold higher in subtropical compared to subantarctic waters, while the rates of picoeukaryotes and nanoeukaryotes had no significant difference between the biogeochemically‐contrasting water masses. Sorted picoeukaryote populations were dominated by Mamiellophyceae, Pelagophyceae, Prymnesiophyceae, and Chrysophyceae, while nanoeukaryote populations were dominated by Dinophyceae and Prymnesiophyceae. Despite significant differences in their taxonomic composition, the sorted picoeukaryote populations in subantarctic waters and nanoeukaryote populations in subtropical and subantarctic waters were dominated by taxa reported in the literature as able to engage in phago‐mixotrophic strategies (Prymnesiophyceae, Chrysophyceae, and Dinophyceae), suggesting that such trophic strategy might be applied by discrete small photosynthetic eukaryote populations to alleviate macronutrient and iron stress.
Sodium dodecyl sulfate‐coated silver nanoparticles accelerate antimicrobial potentials by targeting amphiphilic membranes
Authors: Jin Xiuyan , Peng Na , Cui Aoran , Liu Yue , Peng Xianqi , Huang Linlin , Ed‐Dra Abdelaziz , He Fang , Li Yan , Yang Shikuan , Yue Min 
Published: December 2024

AbstractCompelling concerns about antimicrobial resistance and the emergence of multidrug‐resistant pathogens call for novel strategies to address these challenges. Nanoparticles show promising antimicrobial activities; however, their actions are hindered primarily by the bacterial hydrophilic–hydrophobic barrier. To overcome this, we developed a method of electrochemically anchoring sodium dodecyl sulfate (SDS) coatings onto silver nanoparticles (AgNPs), resulting in improved antimicrobial potency. We then investigated the antimicrobial mechanisms and developed therapeutic applications. We demonstrated SDS‐coated AgNPs with anomalous dispersive properties capable of dispersing in both polar and nonpolar solvents and, further, detected significantly higher bacteriostatic and bactericidal effects compared to silver ions (Ag+). Cellular assays suggested multipotent disruptions targeting the bacterial membrane, evidenced by increasing lactate dehydrogenase, protein and sugar leakage, and consistent with results from the transcriptomic analysis. Notably, the amphiphilic characteristics of the AgNPs maintained robust antibacterial activities for a year at various temperatures, indicating long‐term efficacy as a potential disinfectant. In a murine model, the AgNPs showed considerable biocompatibility and could alleviate fatal Salmonella infections. Collectively, by gaining amphiphilic properties from SDS, we offer novel AgNPs against bacterial infections combined with long‐term and cost‐effective strategies.
Genetics of Nonsyndromic Microtia and Congenital Aural Atresia: A Scoping Review
Authors: Govindan Aparna , Fiest Carly , Chou David W. , Saade Mia , Gray Mingyang , Cosetti Maura 
Published: December 2024

AbstractObjectiveTo review the literature on genetics of nonsyndromic microtia and congenital aural atresia (CAA).Data SourcesEmbase, Ovid (Medline), and Web of Science.Review MethodsThe search was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines for scoping reviews. Included studies were original research studies discussing the genetics or pattern of inheritance of non‐syndromic microtia and/or CAA, defined as microtia and/or CAA that was completely isolated except for the presence of hearing loss.ResultsThirty studies met inclusion criteria, describing 40 unique genes and one susceptibility gene locus (4p15.32‐4p16.2) associated with nonsyndromic microtia, CAA, or microtia and CAA. The 3 most cited genes describing microtia genetics alone were HOXA2, MUC6, and GSC. A single article describing nonsyndromic CAA alone identified the TSHZ1 as a candidate gene. Among 194 subjects from 18 manuscripts describing mendelian inheritance for non‐syndromic microtia or microtia and CAA, 49% of the individuals were found to have autosomal dominant transmission, 4% had autosomal recessive, 5% had X‐linked recessive, and 42% had no reported pattern of inheritance.ConclusionCurrent literature on the genetics of microtia and CAA is largely derived from genetic analysis of syndromic patients. Despite comprising over half of the clinical population, available data on non‐syndromic patients remains limited. Understanding genetic polymorphisms and their correlation to phenotypic data more readily available to otolaryngologists offers the prospect of categorizing severity of anatomic malformation and hearing loss to guide future intervention, and improve ability to provide patient‐ and family‐centered counseling.
Flexible, Lightweight, and Hydrophobic TPU/CNT Nanocomposite Foam With Different Surface Microstructures for High‐Performance Wearable Piezoresistive Sensors
Authors: Huang An , Gu Shengguo , Yang Zhenyu , Chen Xin , He Minghui , Peng Xiangfang 
Published: December 2024

ABSTRACTFlexible piezoresistive pressure sensors, due to their lightweight, bendable, and highly sensitive characteristics, have been widely used in fields such as wearable devices, electronic skin, and intelligent robotics. Besides the development of various high‐performance materials, the performance of these sensors is closely related to the design of their surface microstructures. Different surface microstructures can significantly enhance the sensitivity, stability, and durability of piezoresistive sensors. In this paper, three types of flexible thermoplastic polyurethane (TPU)/carbon nanotube (CNT) nanocomposite foam piezoresistive sensors with different surface microstructures and internal porous structures were prepared using supercritical carbon dioxide (sc‐CO₂) foaming process. The effects of the three surface microstructures on the piezoresistive sensing performance of TPU/CNT nanocomposite foams were studied in detail. The results show that the foam sensor with a double‐ridge surface microstructure exhibits significantly enhanced sensing performance, including high sensitivity (0.309 kPa−1), fast response time (~40 ms), wide working range (0–80 kPa), and stability over more than 600 cycles. Additionally, the prepared flexible piezoresistive sensors can be integrated into smartwatches, fitness bands, and smart clothing, enabling real‐time monitoring of heart rate, blood pressure, respiratory rate, and physical activity. This provides precise data support, demonstrating the promising application prospects of these flexible surface microstructure foam piezoresistive sensors in the future.
ECMHA‐PP: A Breast Cancer Prognosis Prediction Model Based on Energy‐Constrained Multi‐Head Self‐Attention
Authors: Zhang Fan , Liu Chaoyang , Zhang Xinhong 
Published: December 2024

ABSTRACTPurposeBreast cancer is a significant threat to women's health. Precise prognosis prediction for breast cancer can help doctors implement more rational treatment strategies. Artificial intelligence can assist doctors in decision‐making and enhance prediction accuracy.Experimental DesignIn this paper, a deep learning model ECMHA‐PP (Energy Constrained Multi‐Head Self‐Attention based Prognosis Prediction) is proposed to predict the prognosis of breast cancer. ECMHA‐PP utilizes patients' clinical data and extracts features through a cross‐position mix and a channel mix multi‐layer perceptron. Then, it incorporates an energy‐constrained multi‐head self‐attention layer to improve feature extraction capability. The source code of ECMHA‐PP has been hosted on GitHub and is available at https://github.com/xiaoliu166370/ECMHA‐PP.ResultsTo evaluate our proposed method, prognostic prediction experiments were performed on the METABRIC dataset, yielding outstanding results with an average accuracy of 93.0% and an average area under the curve of 0.974. To further validate the model's performance, we conducted tests on another independent dataset, BRCA, achieving an accuracy of 87.6%.Conclusions and Clinical RelevanceIn comparison with other widely used advanced methods, ECMHA‐PP demonstrated higher comprehensive performance, making it a reliable prognostic prediction model for breast cancer. Given its robust feature extraction and prediction capabilities.