bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unsalted tomato juice intake improves blood pressure and serum low‐density lipoprotein cholesterol level in local Japanese residents at risk of cardiovascular disease
Authors: Odai Tamami , Terauchi Masakazu , Okamoto Daisaku , Hirose Asuka , Miyasaka Naoyuki 
Published: May 2019

AbstractThe aim of this study was to investigate the effects of unsalted tomato juice intake on cardiovascular risk markers in local Japanese residents. Four hundred and eighty‐one local residents in Kuriyama, Japan, were enrolled in this study. Throughout the year of the study, they were provided with as much unsalted tomato juice as they wanted. Participants were screened for cardiovascular risk markers, such as blood pressure (BP), serum lipid profile, and glucose tolerance, before and after the study period. Of the study participants, 260 participated in a detailed study of their lifestyle factors. The average ages of the 184 male and 297 female participants were 56.3 ± 13.3 (mean ± SD) and 58.4 ± 11.7 years, respectively. BP in 94 participants with untreated prehypertension or hypertension was significantly lowered (systolic BP, 141.2 ± 12.1–137.0 ± 16.3 mmHg, p = 0.003; diastolic BP, 83.3 ± 10.1–80.9 ± 11.1 mmHg, p = 0.012, paired t test). Further, the serum low‐density lipoprotein cholesterol (LDL‐C) level in 125 participants with untreated dyslipidemia significantly decreased (155.0 ± 23.2–149.9 ± 25.0 mg/dl, p = 0.005, paired t test). These beneficial effects were not different between sexes and among the different age groups. No significant difference in lifestyle was found before and after the study. Unsalted tomato juice intake improved systolic and diastolic BP and serum LDL‐C level in local Japanese residents at risk of cardiovascular conditions.
Ore geology, fluid inclusions, and C–H–O–S–Pb isotopes of Nagengkangqieergou Ag‐polymetallic deposit, East Kunlun Orogen, NW China
Authors: Chen Xiao‐Dong , Li You‐Guo , Li Min‐Tong , Zhou Hong‐Bing , Sun Chong‐Bo , Zhao Yun‐Chuan , Lai Chun‐Kit 
Published: May 2019

Several large Ag‐polymetallic deposits have been newly discovered in the eastern segment of the East Kunlun Orogen, with the largest one being the high‐grade Nagengkangqieergou deposit (3,017 tons Ag at 310.04 g/t). The Ag ore veins are hosted in the Palaeoproterozoic Jinshuikou Group and Upper Triassic Elashan Formation, principally along NW‐trending faults and volcanogenic ring fractures. Alteration and mineralization processes occurred in two periods (J and E), which are hosted in two different ore‐bearing sequences (Jinshuikou Group and Elashan Formation respectively). These two periods can be subdivided into Stages J‐I, J‐II, J‐III, and J‐IV and Stages E‐I, E‐II, and E‐III. Fluid inclusions in the ore‐bearing quartz and calcite comprise predominantly of vapour‐rich two‐phase (vapour + liquid) type and minor pure‐liquid type. Fluid inclusions in Stage J quartz and calcite homogenized mainly at 270–330°C, 250–310°C, 250–290°C, and 170–250°C, with salinities of 5–6 wt.%, 4–6 wt.%, 4–5 wt.%, and 3–5 wt.% NaCl equiv., respectively. Fluid inclusions in Stage E quartz homogenized mainly at 210–250°C, 170–230°C, and 150–170°C, with salinities of 6–7 wt.%, 4–5 wt.%, and 1–2 wt.% NaCl equiv., respectively. δ13C and δ18OSMOW values of the calcite samples are of −6.0‰ to −2.8‰ and 5.2‰ to 20.5‰, respectively. The results indicate a mantle‐derived magmatic source and low temperature alteration. and δDV–SMOW values of the quartz and calcite from the Jinshuikou Group vary from −1.9‰ to 9.0‰ and −116.5‰ to −69.3‰, respectively, whilst those of
quartz from the Elashan Formation vary from −11.6‰ to 7.8‰ and −99.7‰ to −86.7‰. The results indicate that the ore‐forming fluids are of medium‐low temperatures and low salinities and were originated from magmatic water to meteoric water. Sulfide δ34SV–CDT values of the Jinshuikou Group and the Elashan Formation are of −6.1‰ to 0.8‰ and −3.4‰ to 0.9‰, respectively, suggesting a mantle‐derived magmatic source. Sulfide 206Pb/204Pb, 207Pb/204Pb, and 208Pb/204Pb ratios of the J‐period ores (18.190–18.622, 15.598–15.725, and 38.383–39.103, respectively) and E‐period ores (18.408–18.420, 15.746–15.758, and 38.879–38.895, respectively) are similar to those of granodiorite porphyry and Elashan Formation (sub)‐volcanic rocks, respectively, indicative of genetic connections between them. Integrating new evidence from fluid inclusions and C–H–O–S–Pb isotopes, we suggest that (a) mantle‐derived materials and their mixture with crust‐derived ones were critical to the ore formation, (b) the Nagengkangqieergou deposit was formed in a magmatic system and a (sub)‐volcanic system under the syn‐ and post‐collisional tectonics of the East Kunlun Orogen, respectively.
Stress and coping among early childhood intervention professionals receiving reflective supervision: A qualitative analysis
Authors: Frosch Cynthia A. , Mitchell Yolanda T. , Hardgraves Lauren , Funk Sadie 
Published: May 2019

ABSTRACTReflective supervision/consultation (RS/C) is an important component of infant mental health training and practice. Given high levels of job stress reported by a variety of early childhood professionals, the present study offers a qualitative examination of early childhood intervention professionals’ perceptions of stress and coping before and after receiving regular RS/C. Thirty‐one professionals received 9 months of RS/C and completed semistructured interview questionnaires at the pre‐/postassessments. Questionnaires focused on job‐related experiences, including what participants found stressful and how they coped with job‐related stress. Inductive analysis techniques were used to identify themes that arose from the data. Relationships between themes were discovered through axial coding. Three key themes of individual, relational, and organizational stress were identified across the pre‐/postassessments. Similar themes were evident in reports of coping. Following 9 months of RS/C, analysis revealed greater detail and reflection among the majority of participants. These results contribute to the literature through identification of multiple levels of stress and coping as well as areas of continuity and change among participants receiving RS/C. Future research should consider how professionals’ reports of stress and coping relate to reported self‐efficacy and observed competence with young children and families.
Downregulation of IQGAP1 inhibits epithelial‐mesenchymal transition via the HIF1α/VEGF‐A signaling pathway in gastric cancer
Authors: Liu Junqiang , Ni Xiufan , Li Yafang , Chen Mengjiao , Chen Wei , Wu Ying , Chen Baoding , Wu Yan , Xu Min 
Published: May 2019

AbstractAs an oncogene, IQ‐domain GTPase‐activating protein 1 (IQGAP1) regulates the epithelial‐mesenchymal transition (EMT) of several cancers, such as breast cancer, thyroid cancer, and esophageal squamous cell carcinoma. However, the role of the scaffold protein IQGAP1 on EMT in gastric cancer remains unclear. Therefore, the present work was performed to address the question.Our results showed that IQGAP1 expression is upregulated in human gastric cancer specimens and cell lines. Furthermore, IQGAP1 knockdown inhibited the migratory ability of gastric cancer cells and reduced the expression of mesenchymal phenotype markers, including Slug, β‐catenin, Snail, Vimentin, and N‐cadherin, as well as vascular endothelial growth factor‐A (VEGF‐A) secretion in gastric cancer cells. Conversely, IQGAP1 downregulation increased the epithelial phenotype marker E‐cadherin. Furthermore, IQGAP1 silencing not only downregulated hypoxia‐inducible transcription factor 1α (HIF1α) but also limited its translocation from the cytosol to the nucleus.Collectively, our results indicated that EMT was regulated by IQGAP1, which was associated with VEGF‐A, since other data demonstrated that HIF1α was involved in VEGF‐A expression. Therefore, we speculated that IQGAP1 regulated EMT of gastric cancer partially via the HIF1α/VEGF‐A signaling pathway. IQGAP1 may serve as an effective therapeutic biomarker for gastric cancer.
Identification of a nuclear localization signal and importin beta members mediating NUAK1 nuclear import inhibited by oxidative stress
Authors: Palma Mario , Riffo Elizabeth N. , Suganuma Tamaki , Washburn Michael P. , Workman Jerry L. , Pincheira Roxana , Castro Ariel F. 
Published: May 2019

AbstractNUAK1 is a serine/threonine kinase member of the AMPK‐α family. NUAK1 regulates several processes in tumorigenesis; however, its regulation and molecular targets are still poorly understood. Bioinformatics analysis predicted that the majority of NUAK1 localizes in the nucleus. However, there are no studies about the regulation of NUAK1 subcellular distribution. Here, we analyzed NUAK1 localization in several human cell lines, mouse embryo fibroblasts, and normal mouse tissues. We found that NUAK1 is located in the nucleus and also in the cytoplasm. Through bioinformatics analysis and studies comparing subcellular localization of wild type and NUAK1 mutants, we identified a conserved bipartite nuclear localization signal at the N‐terminal domain of NUAK1. Based on mass spectrometry analysis, we found that NUAK1 interacts with importin‐β members including importin‐β1 (KPNB1), importin‐7 (IPO7), and importin‐9 (IPO9). We confirmed that importin‐β members are responsible for NUAK1 nuclear import through the inhibition of importin‐β by Importazole and the knockdown of either IPO7 or IPO9. In addition, we found that oxidative stress induces NUAK1 cytoplasmic accumulation, indicating that oxidative stress affects NUAK1 nuclear transport. Thus, our study is the first evidence of an active nuclear transport mechanism regulating NUAK1 subcellular localization. These data will lead to investigations of the molecular targets of NUAK1 according to its subcellular distribution, which could be new biomarkers or targets for cancer therapies.
The diagnostic efficacy of circulating miRNAs in monitoring the early development of colitis‐induced colorectal cancer
Authors: El‐Daly Sherien M. , Morsy Safaa M. , Medhat Dalia , El‐Bana Mona A. , Latif Yasmin Abdel , Omara Enayat A. , Awadallah Jackleen R. , Gamal‐Eldeen Amira M. 
Published: May 2019

AbstractEarly detection of colorectal cancer and monitoring the progress in colon carcinogenesis stages is essential to reduce mortality. Therefore, there is continuous search for noninvasive biomarkers with high stability and good sensitivity and specificity. miRNAs have attracted attention as promising biomarkers as they are stably expressed in circulation. The aim of our study is to evaluate the aberrant expression of circulating miRNAs during the stepwise progress of colitis‐associated colon cancer. This was accomplished through assessing the expression levels of five miRNAs (miR‐141, miR‐15b, miR‐17‐3p, miR‐21, and miR‐29a) in serum and their corresponding tissue samples through the different cycles of colorectal carcinogenesis cascade using the azoxymethane/dextran sulfate sodium murine model. We also compared the diagnostic performance of these selected miRNAs with the conventional tumor biomarkers CEA and CA 19‐9. The results of our study revealed that the expression levels of those miRNAs were dynamically changing in accordance with the tumor development state. Moreover, their aberrant expression in serum was statistically correlated with that in tissue. Our data also revealed that serum miR‐15b, miR‐21, and miR‐29a showed the best performance in terms of diagnostic power. Our findings highlight the efficiency of these circulating miRNAs not only for early diagnostics purposes, but also for monitoring progress in the colorectal carcinogenesis process, and therefore encouraging integrating these noninvasive biomarkers into the clinical diagnostic settings beside the traditional diagnostic markers for accurate screening of the early progress of colon carcinogenesis.
Coculture with noncardiac cells promoted maturation of human stem cell–derived cardiomyocyte microtissues
Authors: Varzideh Fahimeh , Mahmoudi Elena , Pahlavan Sara 
Published: May 2019

AbstractCardiomyocytes derived from human pluripotent stem cells (hPSC‐CM) provided a promising cell source for cell therapy, drug screening, and disease modeling. However, hPSC‐CM are immature and phenotypically more similar to fetal rather than adult cardiomyocytes in vitro. We explored the impact of coculture of human embryonic stem cell–derived mesenchymal stem cells (hESC‐MSC) and endothelial cells (ECs) with human embryonic stem cells–derived cardiac progenitor cells (hESC‐CPC) on the gene expression and electrophysiological properties of hESC‐CPC in 3D culture (microtissue spheroid). In this regard, hESC‐CPC were cultured either alone (CM microtissue) or in coculture with EC and hESC‐MSC (CMEM microtissue) on agar‐coated 96‐well round‐bottomed plates for 1 week. Lumen‐like structures were formed in CMEM but not in CM microtissue. Cardiac progenitor markers (TBX5, GATA4) were downregulated and cardiac sarcomeric transcripts (MLC2v and β‐MHC) were upregulated in CMEM compared with CM microtissue. Furthermore, beating frequencies, beating cycles, and field potential durations of CMEM resided in the range of adult cardiomyocytes rather than fetal like phenotypes observed in CM microtissue. These findings demonstrated that CPC spheroids in coculture with EC and hESC‐MSC may undergo greater maturation toward an adult‐like cardiomyocyte.
Titanium with nanotopography induces osteoblast differentiation through regulation of integrin αV
Authors: Lopes Helena Bacha , Freitas Gileade Pereira , Fantacini Daianne Maciely Carvalho , Picanço‐Castro Virginia , Covas Dimas Tadeu , Rosa Adalberto Luiz , Beloti Marcio Mateus 
Published: May 2019

AbstractTopographical modifications of titanium (Ti) at the nanoscale level generate surfaces that regulate several signaling pathways and cellular functions, which may affect the process of osseointegration. Here, we investigated the participation of integrin αV in the osteogenic capacity of Ti with nanotopography. Machined titanium discs (untreated) were submitted to treatment with H2SO4/H2O2 to produce the nanotopography (nanostructured). First, the greater osteogenic capacity of the nanotopography that increased osteoblast differentiation of mesenchymal stem cells compared with untreated topography was shown. Also, the nanostructured surface increased (regulation ≥ 1.9‐fold) the gene expression of 6 integrins from a custom array plate utilized to evaluate the gene expression of 84 genes correlated with cell adhesion signaling pathway, including integrin αV, which is involved in osteoblast differentiation. By silencing integrin αV in MC3T3‐E1 cells cultured on nanotopography, the impairment of osteoblast differentiation induced by this surface was observed. In conclusion, it was shown that nanotopography regulates the expression of several components of the cell adhesion signaling pathway and its higher osteogenic potential is, at least in part, due to its ability to upregulate the expression of integrin αV. Together with previous data that showed the participation of integrins α1, β1, and β3 in the nanotopography osseoinduction activity, we have uncovered the pivotal role of this family of membrane receptors in the osteogenic potential of this surface.
S100A8 acts as an autocrine priming signal for heme-induced human Mϕ pro-inflammatory responses in hemolytic inflammation
Authors: Silveira Angélica A A , Mahon Olwyn R , Cunningham Clare C , Corr Emma M , Mendonça Rafaela , Saad Sara T O , Costa Fernando F , Dunne Aisling , Conran Nicola 
Published: May 2019

Abstract
Intravascular hemolysis, in addition to reducing red cell counts, incurs extensive vascular inflammation and oxidative stress. One product of hemolysis, heme, is a potent danger associated molecular pattern (DAMP), activating leukocytes and inducing cytokine expression and processing, among other pro-inflammatory effects. We explored pathways by which heme-induced inflammation may be amplified under sterile conditions. Incubation of human Mϕs, differentiated from CD14+ cells, with heme induced time- and concentration-dependent gene and protein expression of S100A8, a myeloid cell-derived alarmin. Human Mϕ stimulation with recombinant S100A8, in turn, induced robust pro-IL-1β expression that was dependent upon NF-κB activation, gene transcription, and partially dependent upon TLR4-mediated signaling. Moreover, heme itself stimulated significant Mϕ pro-IL-1β gene and protein expression via an S100A8-mediated mechanism and greatly amplified S100A8-driven NLRP3 inflammasome-mediated IL-1β secretion. In vivo, induction of acute intravascular hemolysis in mice induced a rapid elevation of plasma S100A8 that could be abolished by hemopexin, a heme scavenger. Finally, plasma S100A8 levels were found to be significantly elevated in patients with the inherited hemolytic anemia, sickle cell anemia, when compared with levels in healthy individuals. In conclusion, we demonstrate that hemolytic processes are associated with S100A8 generation and that some of the inflammatory effects of heme may be amplified by autocrine S100A8 production. Findings suggest a mechanism by which hemolytic inflammation could be propagated via leukocyte priming by endogenous proteins, even in sterile inflammatory environments such as those that occur in the hemolytic diseases. S100A8 may represent a therapeutic target for reducing inflammation in hemolytic disorders.
Argon plasma effects on maize: pesticide degradation and quality changes
Authors: Feng Xinxin , Ma Xin , Liu Hongxia , Xie Jinzhuo , He Chi , Fan Rui 
Published: May 2019

AbstractBACKGROUNDDuring planting, storage and transportation of maize excessive amounts of pesticides are used to ensure production, resulting in pesticide residues on the maize that can threaten human health. Plasma, compared with other technologies, has been widely regarded as a green, safe and promising technology for surface decontamination to ensure maize safety and quality.RESULTSThe aim of this study is to discuss plasma effects on the degradation of chlorpyrifos and carbaryl on maize surface and the changes of treated maize quality. Results achieved the largest degradation efficiency of chlorpyrifos and carbaryl, up to 91.5% and 73.1%, respectively. The physical changes of maize were observed by scanning electron microscopy (SEM), showing a decrease in contact angle, an increase in surface free energy and polar component, leading to improved hydrophilicity of the treated maize. There was no significant change of vitamin B2 content of maize. A significant increase of acid value and decrease of moisture content and starch content were observed within acceptable limits.CONCLUSIONIt is reasonable to believe that argon plasma treatment enhances the edible safety of maize while maintaining maize quality. © 2019 Society of Chemical Industry
Higher species diversity improves soil water infiltration capacity by increasing soil organic matter content in semiarid grasslands
Authors: Liu Yu , Miao Hai‐Tao , Chang Xiaofeng , Wu Gao‐Lin 
Published: May 2019

AbstractUnderstanding the mechanisms mediating biodiversity effects on ecosystem functions and services is considered a core issue in ecological and environmental sciences. We studied the direct and indirect effects of plant diversity on soil organic carbon storage and soil infiltration capacity in semiarid grasslands. Plant species diversity enhances soil organic carbon and soil infiltration capacity via multiple plant–soil feedback mechanisms in long‐term natural restoration grasslands. Plant species diversity increases community productivity, resulting in increasing soil carbon storage, which improves soil infiltration capacity by influencing soil aggregate stability and porosity. The present study indicates that plant species diversity is conducive to increasing atmospheric CO2 sequestration and reducing the risk of soil erosion. Our study provides a framework for interpreting the relationships among plant species diversity, soil organic carbon, and soil infiltration capacity to understand plant–soil feedback mechanisms in semiarid grasslands.
Efficacy of Combined Intense Pulsed Light (IPL) With Fractional CO2‐Laser Ablation in the Treatment of Large Hypertrophic Scars: A Prospective, Randomized Control Trial
Authors: Daoud Alexander A. , Gianatasio Chloe , Rudnick Ashley , Michael Mary , Waibel JillS 
Published: May 2019

Background and ObjectivesScar rehabilitation is a complex process that incorporates medical, surgical, and physical therapeutic measures to best restore function and visual normalcy. Lasers have emerged as essential tools in the management of scars, with devices available to address scar size, dyschromia, and contour irregularities. As different lasers treat these different features, multi‐laser, same session therapeutic approaches may offer a more comprehensive approach to scar revision. In this study, we aim to demonstrate the effect of a combinatorial, same session treatment with intense pulsed light (IPL) and fractional ablative CO2 as compared to single laser treatment with fractional ablative CO2 laser alone or control in the treatment of mature hypertrophic scars.Materials and MethodsThis institutional review board‐approved, randomized controlled trial, enrolled 23 healthy adults with large (>100 cm2) hypertrophic scars who were randomized to one of three treatment arms: (i) IPL and CO2 ablative fractional laser (AFL), (ii) CO2 AFL alone, and (iii) control (no laser treatment). Subjects underwent a total of four treatment sessions at 6–8‐week intervals with follow‐up visits at 1, 3, and 6 months following the last treatment session. Primary endpoints included blinded scoring of before and after photographs via the Manchester Scar Scale (MSS). Secondary endpoints included the Patient‐Observer Scar Assessment Scale (POSAS).ResultsAs compared to control and CO2 AFL laser alone, the combination of CO2 AFL and IPL demonstrated statistically significant improvement across a greater number of scar domains, as assessed by the MSS. 100% of subjects in both treatment groups showed a statistically significant decrease in the POSAS scale after the series of four treatment sessions. No adverse events were reported.ConclusionTreatment with combined IPL and CO2 AFL demonstrated higher average improvements across the majority of assessed scar domains, as compared to both control and CO2 laser alone. The difference was not statistically significant in overall MSS score as expected due to the role of CO2 as the main source of improvement. However, only the combination group had statistically significant improvement in both color and texture. These findings support the assertion that a multi‐photo‐thermolytic approach with combined IPL and CO2 AFL can have a positive impact on the treatment of hypertrophic scars by using multiple wavelengths to maximize laser‐skin interactions in targeting the different chromophores expressed in scar tissue. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.