bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tumor‐derived lactic acid promotes acetylation of histone H3K27 and differentiation of IL‐10‐producing regulatory B cells through direct and indirect signaling pathways
Authors: Muraoka Satoshi , Baba Takashi , Akazawa Takashi , Katayama Kei‐ichi , Kusumoto Hiroki , Yamashita Shimpei , Kohjimoto Yasuo , Iwabuchi Sadahiro , Hashimoto Shinichi , Hara Isao , Inoue Norimitsu 
Published: November 2024

AbstractTumor cells are known to enhance glycolysis, even under normoxic conditions, via the Warburg effect, producing excess lactic acid in the tumor microenvironment. Lactic acid enhances the IL‐23/IL‐17 pathway and induces chronic inflammation. The acidic microenvironment formed by lactic acid suppresses immune cell proliferation and activation. In the present study, we clarified that lactic acid had two novel activities for immune cells. First, lactic acid specifically enhanced acetylation at lysine 27 of histone H3 (H3K27ac) in splenic B cells and monocytes/macrophages, and this epigenetically up‐regulates the expression of genes. Acetylation and methylation of other residues of histone H3 were rarely induced. Second, lactic acid induced a particularly‐marked enhancement of Il10 gene expression in B cells, leading to an increase in IL‐10‐producing regulatory B (Breg) cells. Furthermore, two pathways should be involved in both the enhancement of H3K27ac and the induction of Breg cells by lactic acid: a direct pathway that enhances the CD40 signal in B cells, and an indirect pathway that affects B cells by activating the exchange protein directly activated by cAMP (EPAC) 1/2 in non‐B cells. In tumor‐bearing mice, the levels of H3K27ac of tumor‐infiltrating B cells were significantly higher than splenic B cells and were suppressed by intraperitoneal injection of the EPAC1/2 inhibitor. In conclusion, tumor‐derived lactic acid increases H3K27ac and IL‐10‐producing Breg cells, causing the suppression of anti‐tumor immunity.
Patient experiences of cancer genetic testing by non‐genetics providers in the surgical setting
Authors: Fiallos Katie , Selznick Erica , Owczarzak Jill , Camp Melissa , Euhus David , Habibi Mehran , Jacobs Lisa , Johnson Angelisa , Klein Catherine , Lange Julie , Njoku Patricia , Visvanathan Kala 
Published: November 2024

AbstractAs indications for hereditary cancer genetic testing (GT) for patients with breast cancer (BC) expand, breast surgery teams offer GT to newly diagnosed patients to inform surgical plans. There is, however, limited data on the experiences of patients undergoing cancer GT by non‐genetic providers. This study used in‐depth interviews with 21 women recently diagnosed with BC at a large academic health system to capture their experiences. Post‐positivist codebook thematic analysis was used to identify major themes from the interviews. Participants reported an overall positive experience of this GT process, stating that they prefer GT at an existing appointment shortly after their diagnosis, even though they described the conversation as brief. Many participants indicated thinking about or desiring GT before the offer was made. Interestingly, most participants did not see surgical decision‐making as the main reason for GT and were instead motivated by concern for relatives and to have complete information. Interview data indicated areas for improvement in patient‐provider communication, and most participants agreed that additional reference information on GT in the form of written or video materials would be helpful. Offering GT at an initial breast surgery appointment is acceptable and desired by patients with a new BC diagnosis and should be considered as a way to increase access to GT for these patients. However, additional information for patients is needed to close gaps in communication and provide a trustworthy reference following a busy medical appointment.
Water, Sanitation, Hygiene Facilities and Economic Well‐Being: A Multilevel and Spatial Analysis in Punjab, Pakistan
Authors: Hussain Muhammad Munawar , Iqbal Asim , Abbas Syed Jaffar 
Published: November 2024

ABSTRACTThis study meticulously explores the intricate relationship between water, sanitation and hygiene (WASH) facilities and economic well‐being in Punjab, employing a comprehensive approach that utilizes Multiple Indicator Cluster Surveys (MICS) data. Existing research lacks comprehensive multilevel and spatial analyses of the effect of WASH facilities on economic well‐being. Addressing a gap in existing research, this study employs both linear and multilevel regression models to analyse household and district‐level factors. The study finds significant associations between improved sanitation, hygiene practices and economic well‐being. A 1% increase in WASH facilities is associated with approximately a 0.52% increase in economic well‐being. Moreover, the enduring influence of parental education and the socio‐economic dynamics within households play pivotal roles. At the district level, factors such as healthcare institutions, a lower multidimensional poverty index and enhanced access to water and sanitation infrastructure emerge as crucial contributors to economic well‐being. The spatial analysis, utilizing Moran's I, further enriches the geographical understanding of these intricate connections. The results suggest a positive spatial correlation of WASH facilities within Punjab. This study attempts to unravel this complex relationship of WASH facilities with economic well‐being, which is very important for further understanding the broader dimensions of socio‐economic factors forming these dynamics. The insights gleaned from this research hold pivotal implications for policymaking, guiding interventions toward inclusive and sustainable socio‐economic well‐being.
Timing of cartilage articulation following impact injury affects the response of surface zone chondrocytes
Authors: Thompson Caroline L. , Bonassar Lawrence J. 
Published: November 2024

AbstractPost‐traumatic osteoarthritis develops following an inciting injury to a joint and results in cartilage degeneration. Mechanical loading, including articulation, drives anabolic responses in cartilage clinically, in vivo, and in vitro. Tribological articulation, or sliding of cartilage on a glass counterface, has long been used as an in vitro tool to study cartilage tissue behavior. However, it is unclear if tribological articulation affects chondrocyte fate following injury, and if the timing of articulation impacts the resultant effect. The goal of this study was to investigate the effect of tribological articulation on injured cartilage tissue at two time points: (i) performed immediately after injury and (ii) 24 h after injury. Neonatal bovine femoral cartilage explants were injured using a rapid spring‐loaded impactor and subsequently subjected to tribological articulation. Cell death due to impact injury was highest near the articular surface, suggesting a strain‐dependent mechanism. Immediate articulation following injury mitigated cell death compared to injury alone or delayed articulation; markers for both general cell death and early‐stage apoptosis were markedly decreased in the explants that were immediately slid. Interestingly, mitigation of cell death due to sliding was most predominant at the cartilage surface. Tribological articulation is known to create fluid flow within the tissue, predominantly at the articular surface, which could drive the protective response seen here. Altogether, this work shows that perturbations to the cellular environment immediately following cartilage injury significantly impact chondrocyte fate.
Postoperative Morbidity Outcomes Associated With Superficial Temporal Versus Cervical Vessels as Recipient Vessels in Head and Neck Reconstruction: A Systematic Review and Meta‐Analysis
Authors: Jeong Woo Shik , Jeong Woonhyeok 
Published: November 2024

ABSTRACTBackgroundThe purpose of this meta‐analysis was to compare the surgical outcomes of head and neck reconstruction via free flap surgery, with neck vessels versus superficial temporal vessels as recipient vessels.MethodsThe PubMed, Embase, and Scopus databases were systematically searched via the following keywords: (“superficial temporal” OR “temporal”) AND (“free flap” OR “free tissue transfer”) AND (“head and neck” OR “face”). The following data were extracted: first author, publication year, flap type, reconstruction region, concordant vein graft, recipient vessel, and postoperative complications, including thrombosis, partial necrosis, and flap failure. The recipient vessels were divided into two groups: the superficial temporal artery (STA)/V group and the neck group.ResultsSix hundred and thirty‐five studies that met the inclusion criteria were included and reviewed systematically for a meta‐analysis. Compared with the neck vessel group, the STA/V vessel group had a significantly greater risk of flap failure (odds ratio: 2.18; 95% CI: 1.32–3.60; p = 0.002), with low heterogeneity (p = 0.84; I2 = 0%). However, there were no significant differences in the rates of thrombosis or partial necrosis.ConclusionsCompared with the use of neck vessels, the use of STA/V vessels as recipient vessels for head and neck reconstruction could increase the risk of total flap necrosis. Considering these findings, surgeons should exercise caution when selecting the STV as the recipient site, and as some authors have suggested, proximal dissection may be necessary during surgery.
Investigations of all optical 2×4 decoder based on PhC structure and nonlinear ring resonators
Authors: Askarian Asghar , Parandin Fariborz 
Published: November 2024

AbstractIn this paper, all optical 2 × 4 decoder (AODEC) is provided using threshold switching method in 2D photonic crystal (PC) structures which has low optical loss and most widely used in photonic integrated circuits (PICs). The proposed optical decoder is designed using silicon pillars with a refractive index of 3.46 perforated in the air background in a cubic lattice with a lattice constant of 585 nm. The proposed structure is composed of seven optical waveguides and three nonlinear ring resonators. Plane wave expansion (PWE) and finite difference time domain (FDTD) methods have been used to obtain and analyze the photonic band gap (PBG) range and performance of the designed AODEC, respectively. The proposed optical device achieves a contrast ratio (CR) of 8.98 dB, time delay of 2 ps and switching speed of 500 GHz at the optical wavelengths of 1550 nm. According to results, high data bit rate and contrast ratio, compactness, low power consumption and ultra‐fast operation are the main advantages of designed 2 × 4 AODEC compared with the previous structures.
Investigation of risk factors associated with the development of depressive symptoms in healthy subjects exposed to long‐term stress: A prospective study of the Japanese Antarctic research expedition wintering party
Authors: Fukunishi Takashi , Ono Miki , Kasuya Kazuhiko , Ishikawa Takashi , Honyashiki Mina , Masuya Jiro , Inoue Takeshi 
Published: November 2024

AbstractBackgroundStressors induce depression together with parenting experienced in childhood, personality traits, and sleep. In this study, we investigated factors associated with the development of depression in a long‐term stressful environment, namely, the Antarctic Research Expedition wintering party, by comparing 2 groups, the depression and nondepression groups.MethodsA self‐administered questionnaire was used to survey 91 members of the Japanese Antarctic Research Expedition who spent winters in the Antarctic base. Psychological evaluations of depression, anxiety, and sleep were performed using a questionnaire every 3 months during the participants’ stay in Antarctica. The primary endpoint was the occurrence of minor or major depression, as evaluated by the PHQ‐9 score.ResultsParticipants with a PHQ‐9 score of 5 or more during their stay in Antarctica were defined as the depression group (25 subjects), and participants with a PHQ score of 4 or less were defined as the nondepression group (43 subjects). Compared with the nondepression group, the depression group had significantly higher scores for predeparture PHQ‐9, state and trait anxiety, sleep disturbance, and neuroticism. Multivariable logistic regression analyses showed that higher predeparture scores of subthreshold depressive symptoms and neuroticism were found to be significant predictors of the occurrence of depression during their stay in Antarctica.ConclusionsThis study prospectively showed that subthreshold depressive symptoms and neuroticism, which were suggested as risk factors in previous studies, were confirmed to be risk factors for depression. The results of our study are expected to contribute to the understanding of depression in harsh environments.
An intelligent system for determining the degree of tree bark beetle damage based on the use of generative‐adversarial neural networks
Authors: Victor Sineglazov , Mykhailo Hordun , Samuli Junttila 
Published: November 2024

AbstractThe increasing prevalence of tree bark beetle infestations poses a significant threat to forest ecosystems, leading to detrimental impacts on biodiversity, carbon storage, and timber resources. This study addresses the pressing need for innovative solutions to enhance forest management practices through early detection and assessment of tree health. We developed an intelligent system leveraging multispectral images and generative‐adversarial networks (GANs) to accurately determine the extent of bark beetle damage. Recognizing the challenges posed by traditional neural networks, which require vast amounts of labeled training data, we proposed a novel approach that utilizes a GAN architecture. In this system, a discriminator functions as a classifier, effectively trained on both real and synthetically generated data. Our methodology not only reduces the dependency on extensive labeled datasets but also enhances the robustness of the classification process. The results indicate a classification accuracy of 87.5%, demonstrating significant promise for improving detection capabilities even with limited training resources. The implications of this research are profound, offering potential benefits for the forestry industry through optimized management strategies and economic gains. Furthermore, our findings contribute to the preservation of critical ecosystem services by providing a means to monitor forest health effectively. However, the responsible implementation of this technology is paramount. Continuous refinement of the model, integration with traditional ecological knowledge, and ensuring transparency and equitable access to the developed system are essential for maximizing societal benefits. Additionally, addressing risks such as misinterpretation of data, overreliance on technology, and privacy concerns is crucial to minimize unintended consequences. Overall, this research presents a significant advancement in the field of forest health monitoring and establishes a foundation for future developments in intelligent ecological management systems.
Cover Image, Volume 62, Issue 21
Authors: Tan Runcheng , Huang Guangqi , Wei Cong , He Zepeng , Zhao Tianyu , Shi Yi , Liu Zhijia , Chen Yongming 
Published: November 2024

The cover image is based on the Article Influence of structural variations in polysarcosine functionalized lipids on lipid nanoparticle‐mediated mRNA delivery by Runcheng Tan et al., https://doi.org/10.1002/pol.20240154
image
SMC for Discrete 2‐D Markov Switching Systems: Genetic Algorithm
Authors: Li Shaowei , Qi Wenhai , Park Ju H. , Cheng Jun , Shi Kaibo 
Published: November 2024

ABSTRACTThe sliding mode control (SMC) strategy based on genetic algorithm is proposed for discrete two‐dimensional (2‐D) Markov switching systems with the Roesser model. By means of common 2‐D sliding surface, the 2‐D SMC law is designed for the underlying system. According to Lyapunov stability criteria, sufficient conditions are established for the asymptotic mean‐square stability of the underlying 2‐D Markov switching systems and the reachability of the sliding region is guaranteed. Utilizing an iteration optimizing algorithm, an effective SMC strategy under genetic algorithm is constructed to minimize the sliding region by searching an ideal sliding gain. Finally, the applicability of the proposed 2‐D SMC strategy is verified through an example.
Dealing with dissention in the ranks: A phylogenetic study of Odontadenieae (Apocynaceae)
Authors: Morales J. Francisco , Endress Mary E. 
Published: November 2024

AbstractThe circumscription of Odontadenieae (Apocynaceae) and its constituent genera (including the infrageneric classifications for Odontadenia) are tested here based on the phylogenetic analyses of 33 nuclear and 164 chloroplast sequences from 33 species, 84 available for the first time. As currently circumscribed, Odontadenieae is monophyletic, with the genera divided into three main clades. However, Odontadenia and the subgenera and sections of its three earlier infrageneric classifications are all non‐monophyletic. Secondatia is nested within Odontadenia, and O. laxiflora is nested in Pentalinoninae. In order to achieve a monophyletic Odontadenia, we propose the recognition of Codonechites and its transfer to Pentalinoninae. Secondatia species are transferred to Odontadenia, and Angadenia is reduced to a synonym of Pentalinon. Our results support the inclusion of Elytropus in Mesechiteae and the recognition of three sections in Odontadenia. The status of O. sect. Anomalae cannot be evaluated at this time, and is treated as incertae sedis. Eight genera and 39 species are recognized in the tribe here. In addition to the previously published Pentalinoninae, two new subtribes, Cycladeniinae and Odontadeniinae, are published here to accommodate the taxa in the corresponding clades recovered in this study.
Growth velocity of fetal sacrococcygeal teratoma as predictor of perinatal morbidity and mortality: multicenter study
Authors: Vinit N. , Benachi A. , Rosenblatt J. , Jouannic J.‐M. , Rousseau V. , Bonnard A. , Irtan S. , Fouquet V. , Ville Y. , Khen‐Dunlop N. , Lapillonne A. , Jais J.‐P. , Beaudoin S. , Salomon L. J. , Sarnacki S. 
Published: November 2024

ABSTRACTObjectiveTo identify prenatal predictors of poor perinatal outcome in fetuses with isolated sacrococcygeal teratoma (SCT).MethodsThis was a retrospective study of fetuses with isolated (non‐syndromic) SCT managed at one of five pediatric surgery and/or fetal medicine centers between January 2007 and December 2017. The primary outcome was the occurrence of poor perinatal outcome, defined as prenatal death (including termination), or neonatal death or severe compromise (hemorrhagic shock). Data regarding prenatal diagnosis (sonographic features both at referral and at the last ultrasound examination before pregnancy outcome, assessment of SCT growth velocity), perinatal complications and outcome, and neonatal course were analyzed to determine prenatal SCT characteristics associated with adverse perinatal outcome.ResultsFifty‐five fetuses were included, diagnosed with isolated SCT at a median gestational age of 22 (interquartile range, 18–23) weeks. There was a poor perinatal outcome in 31% (n = 17) of these cases, including intrauterine fetal demise (4%, n = 2), pregnancy termination (13%, n = 7) and neonatal severe compromise (15%, n = 8), leading to neonatal death in five cases. The overall survival rate after prenatal diagnosis of isolated SCT was 75% (n = 41 of 55). Earlier gestational age at diagnosis (P = 0.02), large tumor volume at referral (P < 0.001), presence of one or more hemodynamic complications (P = 0.02), fast tumor growth velocity (P < 0.001) and high tumor grade (highest tumor grade ≥ 3) (P = 0.049) were associated with poor perinatal outcome on univariate analysis. On stepwise logistic regression analysis, tumor growth velocity was the only remaining independent factor associated with poor perinatal outcome (odds ratio (OR) (per 1‐mm/week increase), 1.48 (95% CI, 1.22–1.97), P = 0.001). The best predictive cut‐off of tumor growth velocity for poor perinatal outcome was 7 mm/week (OR, 25.7 (95% CI, 5.6–191.3), P < 0.001), yielding a sensitivity of 88% and a specificity of 77%.ConclusionsApproximately 30% of fetuses with a diagnosis of isolated SCT have poor perinatal outcome. Tumor growth velocity ≥ 7 mm/week appears to be an appropriate discriminative cut‐off for poor perinatal outcome. These results could help to inform prenatal management and counseling of parents with an affected pregnancy. © 2024 The Author(s). Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.
Spatial variability of hydraulic parameters of a cropped soil using horizontal crosshole ground penetrating radar
Authors: Lärm Lena , Weihermüller Lutz , Rödder Jan , van der Kruk Jan , Vereecken Harry , Klotzsche Anja 
Published: November 2024

AbstractSoil hydraulic parameters (SHP) play a crucial role controlling the spatiotemporal distribution of water in the soil–plant continuum and thus affect water availability for crops. To provide reliable information on the SHP at different scales, measurement techniques with a good spatial resolution and low labor costs are required. In this study, we used crosshole ground penetrating radar (GPR)‐derived soil water contents (SWCs) measured along horizontal rhizotubes under a controlled experimental test site cropped with winter wheat to estimate the unimodal and dual‐porosity soil hydraulic characteristics with different soil layer setups. Therefore, sequential inversion of the GPR‐derived SWCs was performed using the hydrological model HYDRUS‐1D, whereby the SWC data were either averaged prior inversion or used in a spatially distributed way. To analyze if the time‐lapse gathered GPR data contain enough information to estimate the SHP, additional synthetic studies were performed increasing the data resolution to daily GPR measurements. The results showed that the time‐lapse data contained enough information to estimate the SHP accurately. Additionally, spatially distributed soil hydraulic characteristics differed from the one estimated based on averaged SWCs derived from spatially distributed GPR data. Finally, we derived spatially resolved SHP, which can be used for 3D process rhizosphere processes and root–soil interaction modeling.