bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of a complex workplace dietary intervention on Irish employees’ off-duty dietary intakes
Authors: Fitzgerald Sarah , Buckley Lynn , Perry Ivan J , Geaney Fiona 
Published: May 2019

AbstractA paucity of evidence exists regarding the impact of workplace dietary interventions on employees’ off-duty dietary intakes. This study assessed the impact of workplace dietary interventions that included nutrition education and environmental dietary modification both alone and in combination on employees’ dietary intakes inside (on-duty) and outside (off-duty) of work. A pre–post study on employees’ on and off-duty dietary intakes was undertaken. Data were obtained from a complex workplace dietary intervention study (Food Choice at Work Trial). Four manufacturing workplaces were allocated to: Control (n = 111), nutrition education (n = 226), environmental dietary modification (n = 113) and nutrition education and environmental dietary modification combined (n = 400) (2013–14). Seven- to nine-month follow-up data were obtained for 517 employees (61% response) [Control (n = 67), Education (n = 107), Environment (n = 71) and Combined (n = 272)]. Dietary intakes were measured using 24-h dietary recalls. Differences between on and off-duty mean dietary intakes were compared and regression analyses adjusted for potential confounders. Significant reductions in on-duty intakes of total fat (−14.2 g/day, p = 0.000), saturated fat (−7 g/day, p = 0.000), salt (−1.4 g/day, p = 0.000) and total sugars (−8.9 g/day, p = 0.003) were observed in the Combined and in the Environment [total fat (−11.4 g/d, p = 0.017) and saturated fat (−8.8 g/day, p = 0.000)]. In the Combined, significant changes were also observed in off-duty intakes of total fat (−10.0 g/day, p = 0.001), saturated fat (−4.2 g/day, p = 0.001), salt (−0.7 g/day, p = 0.020) and total sugars (−8.1 g/day, p = 0.020). Food service can have a positive impact in our everyday environments, including inside and outside of work. Dietary interventions combining nutrition education and environmental dietary modification can improve employees’ on and off-duty dietary intakes.
To fight another day: France between the fight against terrorism and future warfare
Authors: Pannier Alice , Schmitt Olivier 
Published: May 2019

Abstract
This article examines the ways in which recent military experiences have affected France's approach to the use of military power, the role of allies and its vision of future warfare. In its management of strategic challenges, we identify the persistence of many traits of France's historical habits and practices. France remains a distinct, outward looking, and militarily willing and able European power. However, the threats that France has sought to address and the operational and financial constraints it has faced in the past decade in particular, have led to significant changes in its approach to and conduct of warfare. In particular, the threat of Islamist terrorism has led to a reframing of French governments' priorities around more narrowly-defined national interests. It has translated into a ‘pragmatic’, or ‘realist’ turn in foreign policy, and a move from ‘wars of choice’ to ‘wars of necessity’. In this context, France's military alliances are being rethought around a core number of functional partnerships to compensate for capability gaps and military overstretch. Meanwhile, French armed forces are getting prepared to face the challenges posed by emerging technologies and the future of Great-Power competition. Overall, the multiple security challenges faced by successive French governments have confirmed, yet redefined, the contours of France's traditional dilemma between a desire for an autonomous defense policy and the reality of a necessary reliance on allies.
Compliant Substrates Disrupt Elastic Energy Storage in Jumping Tree Frogs
Authors: Reynaga Crystal M , Eaton Caitrin E , Strong Galatea A , Azizi Emanuel 
Published: May 2019

AbstractArboreal frogs navigate complex environments and face diverse mechanical properties within their physical environment. Such frogs may encounter substrates that are damped and absorb energy or are elastic and can store and release energy as the animal pushes off during take-off. When dealing with a compliant substrate, a well-coordinated jump would allow for the recovery of elastic energy stored in the substrate to amplify mechanical power, effectively adding an in-series spring to the hindlimbs. We tested the hypothesis that effective use of compliant substrates requires active changes to muscle activation and limb kinematics to recover energy from the substrate. We designed an actuated force platform, modulated with a real-time feedback controller to vary the stiffness of the substrate. We quantified the kinetics and kinematics of Cuban tree frogs (Osteopilus septentrionalis) jumping off platforms at four different stiffness conditions. In addition, we used electromyography to examine the relationship between muscle activation patterns and substrate compliance during take-off in a knee extensor (m. cruralis) and an ankle extensor (m. plantaris). We find O. septentrionalis do not modulate motor patterns in response to substrate compliance. Although not actively modulated, changes in the rate of limb extension suggest a trade-off between power amplification and energy recovery from the substrate. Our results suggest that compliant substrates disrupt the inertial catch mechanism that allows tree frogs to store elastic energy in the tendon, thereby slowing the rate of limb extension and increasing the duration of take-off. However, the slower rate of limb extension does provide additional time to recover more energy from the substrate. This work serves to broaden our understanding of how the intrinsic mechanical properties of a system may broaden an organism’s capacity to maintain performance when facing environmental perturbations.
Subsampled inexact Newton methods for minimizing large sums of convex functions
Authors: Bellavia Stefania , Krejić Nataša , Krklec Jerinkić Nataša 
Published: May 2019

Abstract
This paper deals with the minimization of a large sum of convex functions by inexact Newton (IN) methods employing subsampled functions, gradients and Hessian approximations. The conjugate gradient method is used to compute the IN step and global convergence is enforced by a nonmonotone line-search procedure. The aim is to obtain methods with affordable costs and fast convergence. Assuming strongly convex functions, R-linear convergence and worst-case iteration complexity of the procedure are investigated when functions and gradients are approximated with increasing accuracy. A set of rules for the forcing parameters and subsample Hessian sizes are derived that ensure local q-linear/q-superlinear convergence of the proposed method. The random choice of the Hessian subsample is also considered and convergence in the mean square, both for finite and infinite sums of functions, is proved. Finally, the analysis of global convergence with asymptotic R-linear rate is extended to the case of the sum of convex functions and strongly convex objective function. Numerical results on well-known binary classification problems are also given. Adaptive strategies for selecting forcing terms and Hessian subsample size, streaming out of the theoretical analysis, are employed and the numerical results show that they yield effective IN methods.
Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation1
Authors: Xu Qiushi , Li Xiaobing , Ma Li , Loor Juan J , Coleman Danielle N , Jia Hongdou , Liu Guowen , Xu Chuang , Wang Yazhe , Li Xinwei 
Published: May 2019

AbstractKetosis is a major metabolic disorder of high-yielding dairy cows during the transition period. Although metabolic adaptations of the adipose tissue are critical for a successful transition, beyond lipolysis, alterations within adipose tissue during ketosis are not well known. The objective of this study was to investigate the adipose tissue proteome of healthy or ketotic postpartum cows to gain insights into biological adaptations that may contribute to disease outcomes. Adipose tissue biopsy was collected on 5 healthy and 5 ketotic cows at 17 (±4) d postpartum and ketosis was defined according to the clinical symptoms and serum β-hydroxybutyrate concentration. Morphology micrographs stained by hematoxylin–eosin showed that adipocytes were smaller in ketotic cows than in healthy cows. The isobaric tag for relative and absolute quantification was applied to quantitatively identify differentially expressed proteins (DEP) in the adipose tissue. We identified a total of 924 proteins, 81 of which were differentially expressed between ketotic and healthy cows (P < 0.05 and fold changes >1.5 or <0.67). These DEP included enzymes and proteins associated with various carbohydrate, lipid, and amino acid metabolism processes. The top pathways differing between ketosis and control cows were glycolysis/gluconeogenesis, glucagon signaling pathway, cysteine and methionine metabolism, biosynthesis of amino acids, and the cGMP–PKG signaling pathway. The identified DEP were further validated by western blot and co-immunoprecipitation assay. Key enzymes associated with carbohydrate metabolism such as pyruvate kinase 2, pyruvate dehydrogenase E1 component subunit α), lactate dehydrogenase A , phosphoglucomutase 1, and 6-phosphofructokinase 1 were upregulated in ketotic cows. The expression and phosphorylation state of critical regulators of lipolysis such as perilipin-1 and hormone-sensitive lipase were also upregulated in ketotic cows. Furthermore, key proteins involved in maintaining innate immune response such as lipopolysaccharide binding protein and regakine-1 were downregulated in ketotic cows. Overall, data indicate that ketotic cows during the transition period have altered carbohydrate, lipid metabolism, and impaired immune function in the adipose tissue. This proteomics analysis in adipose tissue of ketotic cows identified several pathways and proteins that are components of the adaptation to ketosis.
Dynamic modeling of nutrient use and individual requirements of lactating sows1
Authors: Gauthier Raphaël , Largouët Christine , Gaillard Charlotte , Cloutier Laetitia , Guay Frédéric , Dourmad Jean-Yves 
Published: May 2019

AbstractNutrient requirements of sows during lactation are related mainly to their milk yield and feed intake, and vary greatly among individuals. In practice, nutrient requirements are generally determined at the population level based on average performance. The objective of the present modeling approach was to explore the variability in nutrient requirements among sows by combining current knowledge about nutrient use with on-farm data available on sows at farrowing [parity, BW, backfat thickness (BT)] and their individual performance (litter size, litter average daily gain, daily sow feed intake) to estimate nutrient requirements. The approach was tested on a database of 1,450 lactations from 2 farms. The effects of farm (A, B), week of lactation (W1: week 1, W2: week 2, W3+: week 3 and beyond), and parity (P1: 1, P2: 2, P3+: 3 and beyond) on sow performance and their nutrient requirements were evaluated. The mean daily ME requirement was strongly correlated with litter growth (R2 = 0.95; P < 0.001) and varied slightly according to sow BW, which influenced the maintenance cost. The mean daily standardized ileal digestible (SID) lysine requirement was influenced by farm, week of lactation, and parity. Variability in SID lysine requirement per kg feed was related mainly to feed intake (R2 = 0.51; P < 0.001) and, to a smaller extent, litter growth (R2 = 0.27; P < 0.001). It was lowest in W1 (7.0 g/kg), greatest in W2 (7.9 g/kg), and intermediate in W3+ (7.5 g/kg; P < 0.001) because milk production increased faster than feed intake capacity did. It was lower for P3+ (6.7 g/kg) and P2 sows (7.3 g/kg) than P1 sows (8.3 g/kg) due to the greater feed intake of multiparous sows. The SID lysine requirement per kg of feed was met for 80% of sows when supplies were 112 and 120% of the mean population requirement on farm A and B, respectively, indicating higher variability in requirements on farm B. Other amino acid and mineral requirements were influenced in the same way as SID lysine. The present modeling approach allows to capture individual variability in the performance of sows and litters according to farm, stage of lactation, and parity. It is an initial step in the development of new types of models able to process historical farm data (e.g., for ex post assessment of nutrient requirements) and real-time data (e.g., to control precision feeding).
Sublethal Effects of Insect Growth Regulators on Boll Weevil (Coleoptera: Curculionidae)
Authors: Rolim Guilherme G , Barros Eduardo M , Barbosa Paulo R R , Arruda Lucas S , Torres Jorge B 
Published: May 2019

Abstract
Immature stages of the boll weevil, Anthonomus grandis grandis Boh. (Coleoptera: Curculionidae), develop protected inside cotton fruiting structures. Therefore, the adult beetles have become the main target of insecticide applications. The use of insect growth regulators (IGRs) is recommended against immatures, even though they may also affect the survival and reproductive traits of adult insects. The present study evaluated the impact of a juvenile hormone analog (pyriproxyfen), an ecdysteroid agonist (methoxyfenozide), and a chitin biosynthesis inhibitor (lufenuron) on adult cotton boll weevils, a key cotton pest. Mated and virgin beetles were treated by feeding them contaminated squares and cotton leaf discs that were previously immersed into pyriproxyfen, methoxyfenozide, and lufenuron solutions at field-rate concentrations. After exposure, treated couples were caged onto cotton plants, and survival, fecundity, and egg viability were evaluated. The IGRs neither affected the survival nor fecundity of adult boll weevils. On the other hand, egg viability was significantly reduced by lufenuron, regardless of whether the females were treated premating or postmating or whether their pairs were either treated or untreated. However, egg viability increased as the females aged since the initial exposure date to lufenuron, indicating a potential transovarial effect of this insecticide. Our results indicate that pyriproxyfen and methoxyfenozide do not affect adult boll weevils, whereas lufenuron temporarily reduces the egg viability of this key cotton pest.
Phytoglobin overexpression promotes barley growth in the presence of enhanced level of atmospheric nitric oxide
Authors: Zhang Jiangli , Buegger Franz , Albert Andreas , Ghirardo Andrea , Winkler Barbro , Schnitzler Jörg-Peter , Hebelstrup Kim Henrik , Durner Jörg , Lindermayr Christian 
Published: May 2019

AbstractTo investigate the effect of high atmospheric NO concentrations on crop plants and the role of phytoglobins under these conditions, we performed a long-term study on barley ‘Golden Promise’ wild type (WT), class 1 phytoglobin knockdown (HvPgb1.1−) and class 1 phytoglobin overexpression (HvPgb1.1+) lines. Plants were cultivated with nitrogen-free nutrient solution during the entire growth period and were fumigated with different NO concentration (ambient, 800, 1500, and 3000 ppb). Analysis of fresh weight, stem number, chlorophyll content, and effective quantum yield of PSII showed that NO fumigation promoted plant growth and tillering significantly in the HvPgb1.1+ line. After 80 d of NO fumigation, dry matter weight, spikes number, kernel number, and plant kernel weight were significantly increased in HvPgb1.1+ plants with increasing NO concentration. In contrast, yield decreased in WT and HvPgb1.1− plants the higher the NO level. Application of atmospheric 15NO and 15NO2 demonstrated NO specificity of phytoglobins. 15N from 15NO could be detected in RNA, DNA, and proteins of barley leaves and the 15N levels were significantly higher in HvPgb1.1+ plants in comparison with HvPgb1.1− and WT plants. Our results demonstrate that overexpression of phytoglobins allows plants to more efficiently use atmospheric NO as N source.
Enzyme-free optical DNA mapping of the human genome using competitive binding
Authors: Müller Vilhelm , Dvirnas Albertas , Andersson John , Singh Vandana , KK Sriram , Johansson Pegah , Ebenstein Yuval , Ambjörnsson Tobias , Westerlund Fredrik 
Published: May 2019

Abstract
Optical DNA mapping (ODM) allows visualization of long-range sequence information along single DNA molecules. The data can for example be used for detecting long range structural variations, for aiding DNA sequence assembly of complex genomes and for mapping epigenetic marks and DNA damage across the genome. ODM traditionally utilizes sequence specific marks based on nicking enzymes, combined with a DNA stain, YOYO-1, for detection of the DNA contour. Here we use a competitive binding approach, based on YOYO-1 and netropsin, which highlights the contour of the DNA molecules, while simultaneously creating a continuous sequence specific pattern, based on the AT/GC variation along the detected molecule. We demonstrate and validate competitive-binding-based ODM using bacterial artificial chromosomes (BACs) derived from the human genome and then turn to DNA extracted from white blood cells. We generalize our findings with in-silico simulations that show that we can map a vast majority of the human genome. Finally, we demonstrate the possibility of combining competitive binding with enzymatic labeling by mapping DNA damage sites induced by the cytotoxic drug etoposide to the human genome. Overall, we demonstrate that competitive-binding-based ODM has the potential to be used both as a standalone assay for studies of the human genome, as well as in combination with enzymatic approaches, some of which are already commercialized.
Differential trajectories of neurocognitive functioning in females versus males following treatment for pediatric brain tumors
Authors: Bledsoe Jesse C , Breiger David , Breiger Micah , Shonka Sophia , Ermoian Ralph P , Ojemann Jeffrey G , Werny David M , Leary Sarah E S , Geyer J Russell 
Published: May 2019

AbstractBackgroundFemale and male trajectories of cerebellar and lobar brain structures are sexually dimorphic, making sex a potential candidate moderator of neurocognitive late effects from radiation treatment. We sought to evaluate longitudinal neurocognitive functioning in male versus female children treated for posterior fossa brain tumors.MethodsFifty-one female and 63 male survivors of posterior fossa tumors completed neuropsychological testing at 2 timepoints. We included patients treated with surgical resection, chemotherapy, and radiation therapy. Multilevel mixed modeling was used to predict IQ score as a function of patient sex following treatment (~2 or ~4 years post treatment). Effect sizes were used as a measure of clinical significance.ResultsMultilevel models resulted in a significant sex by time interaction (F = 6.69, P = 0.011). Females’ cognitive scores were considerably higher compared with males at 4 years posttreatment. Females demonstrated an average improvement of 7.61 standard score IQ points compared with a decline of 2.97 points for males at 4 years follow-up. Effect sizes for female IQ compared with male IQ at 4 years posttreatment were between 0.8 and 0.9.ConclusionTrajectories of neurocognitive functioning following posterior fossa tumor treatment differed between female and male children. Sexual dimorphism in radiation late effects may alter treatment decisions in children. Research into sex-specific neuroprotective mechanisms underlying neurocognitive development following pediatric brain tumor treatments is warranted.
How Is Exposure to Tobacco Outlets Within Activity Spaces Associated With Daily Tobacco Use Among Youth? A Mediation Analysis
Authors: Kowitt Sarah D , Lipperman-Kreda Sharon 
Published: May 2019

Abstract

Introduction
We investigated associations between exposure to tobacco outlets within activity spaces and daily tobacco use, and whether exposure to adults or peers using tobacco mediate these relationships.

Methods
We used Geographic Ecological Momentary Assessment data over 14 days from 85 youth aged 16–20 years in eight mid-sized California city areas. Tobacco outlet addresses and global positioning systems locations were geocoded and activity spaces were constructed by joining sequential points. We assessed daily number of tobacco outlets within 50 or 100 m of activity space polylines and number of minutes participants were within 50 or 100 m of tobacco outlets each day; daily use of tobacco; and whether participants saw (1) adults and (2) people their age (peers) using tobacco each day.

Results
Controlling for demographics, results of multilevel structural equation models showed no association between number of tobacco outlets within 50 m of polylines and tobacco use (probit regression coefficient: 0.01, p = .82). However, we found evidence of an indirect effect (p = .001) through daily exposure to peers using tobacco. Specifically, greater number of tobacco outlets within 50 m of polylines was positively associated with seeing peers use tobacco (probit regression coefficient: 0.10, p &lt; .001). In turn, seeing peers use tobacco was positively associated with tobacco use on that day (probit regression coefficient: 2.23, p &lt; .001). Similar results were found for number of tobacco outlets within 100 m of polylines.

Conclusions
Exposure to tobacco outlets within activity spaces affects youth tobacco use through daily exposure to peers who use tobacco.

Implications
Using real-time-ordered data, this article examines whether exposure to adults and peers using tobacco mediate associations between exposure to tobacco outlets within activity spaces and daily tobacco use among youth. Results suggest that exposure to tobacco outlets within activity spaces affects daily tobacco use through exposure to peers who use tobacco. These findings provide additional significant support for policy makers who are considering regulating the number and density of tobacco retailers and point to the importance of interventions focused on peer tobacco use and youths’ daily environments to reduce tobacco use.
Rice DNA-Binding One Zinc Finger 24 (OsDOF24) Delays Leaf Senescence in a Jasmonate-Mediated Pathway
Authors: Shim Yejin , Kang Kiyoon , An Gynheung , Paek Nam-Chon 
Published: May 2019

AbstractLeaf senescence is the final stage of leaf development and in cereal crops, the timing of senescence relative to grain filling has major effects on agronomic traits such as yield. Although many genetic factors are involved in the regulation of leaf senescence in cereals, the key regulators remain to be determined. Plant transcription factors with a conserved DOF (DNA-binding one zinc finger) domain play roles in multiple physiological processes. Here, we show a novel function for OsDOF24 as a repressor of leaf senescence in rice (Oryza sativa). In wild-type leaves, OsDOF24 expression rapidly decreased during natural senescence (NS) and dark-induced senescence (DIS). The gain-of-function mutant osdof24-D, which contains an enhancer-trap T-DNA in the OsDOF24 promoter, exhibited delayed leaf yellowing during NS and DIS. Transgenic plants overexpressing OsDOF24 showed the same phenotype during DIS. Reverse-transcription quantitative real-time PCR analysis revealed that senescence-associated genes (Osl85, Osl57 and OsNAP) and chlorophyll degradation genes (NYC1, NYC3 and SGR) were downregulated in the osdof24-D mutant during dark incubation. Among the phytohormones, only methyl jasmonate induced OsDOF24 expression. Furthermore, the reduced expression of jasmonate biosynthesis-related genes (OsLOX2, OsLOX8, OsHI-LOX, OsAOS1 and OsAOS2) in osdof24-D decreased endogenous jasmonate levels, resulting in delayed leaf senescence under DIS conditions. Yeast one-hybrid assays showed that OsDOF24 binds to the promoter region of OsAOS1. Taken together, our results demonstrate that OsDOF24 suppresses the induction of leaf senescence during vegetative growth by deactivating jasmonate biosynthetic pathways.