bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phototropin switches between cis‐ and trans‐autophosphorylation in light‐induced chloroplast relocation in Marchantia polymorpha
Authors: Noguchi Minoru , Noda Saki , Matsubayashi Yoshikatsu , Kodama Yutaka 
Published: December 2024

SUMMARYIn the accumulation response, chloroplasts move toward weak blue light (BL) to maximize photosynthetic efficiency; in the avoidance response, they move away from strong BL to reduce photodamage. The BL receptor kinase phototropin (phot) mediates these chloroplast relocation responses, and the chloroplast relocation response requires phot kinase activity. Upon receiving BL, phot undergoes autophosphorylation; however, the molecular mechanisms that regulate chloroplast relocation through phot autophosphorylation remain unclear. In this study, we conducted biochemical experiments using phot in the liverwort Marchantia polymorpha and revealed that phot employs cis‐autophosphorylation under weak BL and both cis‐ and trans‐autophosphorylation under strong BL. Inhibiting trans‐autophosphorylation reduced phot autophosphorylation and suppressed the avoidance response, but not the accumulation response. These findings suggest that phot employs two modes of autophosphorylation to alternate between the accumulation and avoidance responses in plants.
The number of positive solutions for n$n$‐coupled elliptic systems
Authors: Jing Yongtao , Liu Haidong , Liu Yanyan , Liu Zhaoli , Wei Juncheng 
Published: December 2024

AbstractWe study the number of positive solutions to the ‐coupled elliptic system
where , , , , , and for . We prove new multiplicity and uniqueness results for positive solutions of the system, whether the system has a variational structure or not. In some cases, we provide a rather complete characterization on the exact number of positive solutions. The results we obtain reveal that the positive solution set of this system has very different structures in the three cases , , and . Moreover, when , very different structures of the positive solution set can also be seen in the case where close to 2 and the case where close to . Similar results are given for elliptic systems with subcritical Sobolev exponents. These results substantially generalize and improve existing results in the literature. To show the effect of the uniqueness result, we apply it to prove existence of a positive solution to a 2‐coupled nonlinear Schrödinger system with critical exponent and potentials.
COVID‐19 and Pregnancy Outcomes: A Descriptive Study From a Tertiary Hospital in Ras Al Khaimah, UAE
Authors: M. Sami Manal , Al Zuheiri Shatha , Sabaneh Nour K. , Amir Abdul Latif Mustafa , Al-Blooshi Shooq Yousef , Osman Mira 
Published: December 2024

Background: Over 768 million cases of COVID‐19 infection have been reported worldwide, with pregnant women being the most vulnerable members of society during such an infectious disease outbreak. In the United Arab Emirates, there are limited studies explaining the effects of COVID‐19 on pregnant women and their fetuses. In this study, the maternal and fetal outcomes in pregnant women with COVID‐19 in a tertiary maternal hospital, United Arab Emirates, were examined.Materials and Methods: A descriptive study was conducted in a tertiary hospital for Obstetrics and Gynecology in Ras Al Khaimah, UAE. The study included all pregnant women who tested positive for COVID‐19 infection from April 2020 to September 2021.Results: The study revealed that a higher number of COVID‐19‐infected pregnant patients presented in their third trimester (69.1%). The comorbidity of body mass index (BMI) had the most effect on the severity/hospitalization status of the patients (p = 0.018). In the nonhospitalized group, fever was the most common symptom (26%), whereas in the hospitalized group, cough was the most common symptom (94%). Emergency cesarean delivery was found to be significant (p = 0.0007) in hospitalized patients. COVID‐19 pneumonia was the prevailing adverse maternal outcome. NICU admission and prematurity were the most frequent neonatal outcomes.Conclusions: In conclusion, our findings show that adverse maternal outcomes, obesity, and mode of delivery were related to COVID‐19 severity in pregnant patients. However, there was no effect generally on the adverse fetal outcomes except for jaundice and birth weight.
Figure 5 from Ruthenium Drug BOLD-100 Regulates <i>BRAF</i>MT Colorectal Cancer Cell Apoptosis through AhR/ROS/ATR Signaling Axis Modulation
Authors: Griffin Daryl , Carson Robbie , Moss Debbie , Sessler Tamas , Lavin Deborah , Tiwari Vijay K. , Karelia Shivaali , Kennedy Richard , Savage Kienan I. , McDade Simon , Carie Adam , Pankovich Jim , Bazett Mark , Van Schaeybroeck Sandra 
Published: December 2024

<p>Oncogenic <i>BRAF</i> regulates BOLD-100 induced ROS, ATR/CHK1 activation and cell death. <b>A,</b> HT-29 and VACO432 cells were treated with BOLD-100 for the indicated times and pATR<sup>T1989</sup>, ATR, pKAP1<sup>S824</sup>, KAP1, pCKH1<sup>S345</sup>, CHK1, and γH2AX levels determined by WB. <b>B,</b> HT-29 cells were preincubated with 1umol/l vemurafenib for 3 hours and thereafter treated with 50 μmol/L BOLD-100 for 48 hours and pATR<sup>T1989</sup>, ATR, pKAP1<sup>S824</sup>, KAP1, pCKH1<sup>S345</sup>, CHK1, γH2AX, pMEK1/2<sup>S217/221</sup>, MEK, PARP, and cleaved caspase-3 expression determined by WB. <b>C,</b> WB analyzes for pATR<sup>T1989</sup>, ATR, pCKH1<sup>S345</sup>, CHK1, γH2AX, PARP, and BRAF in VT1 cells transiently transfected with 1 µg of BRAFV600E expression construct for 12 hours followed by treatment with BOLD-100 for the indicated times. <b>D,</b> VACO432 and HT-29 cells (top) were treated with BOLD-100 for indicated times and ROS levels assessed using the ROS-Glo H<sub>2</sub>O<sub>2</sub> Assay Kit. ROS detection (bottom left) by flow cytometry in VACO432 and VT1 cells following treatment with 50 μmol/L BOLD-100 for 48 hours. ROS detection (bottom middle/right) by flow cytometry in VACO432 and HT-29 cells, preincubated with vemurafenib for 3 hours, followed by 48 hours treatment with 50 μmol/L BOLD-100. MFI, mean fluorescence intensity. <b>E,</b> Cells were preincubated with NAC for 6 hours and thereafter treated with BOLD-100 for 48 hours and ROS levels assessed using the ROS-Glo H<sub>2</sub>O<sub>2</sub> Assay Kit. <b>F,</b> Cells were preincubated with 5 mmol/L NAC for 6 hours and thereafter treated with BOLD-100 for the indicated times and γH2AX, pATR<sup>T1989</sup>, ATR, pKAP1<sup>S824</sup>, KAP1, pCKH1<sup>S345</sup>, and CHK1 levels determined by WB. <b>G,</b> Colorectal cancer cells were preincubated with 5 mmol/L NAC for 6 hours and thereafter treated with BOLD-100 for 48 hours and apoptosis was assessed by WB analysis for PARP (left) and caspase-3/7 activity assays (right). CONT, control; VEM, vemurafenib; B-100, BOLD-100.</p>
Figure S15 from CD91 and Its Ligand gp96 Confer Cross-Priming Capabilities to Multiple APCs during Immune Responses to Nascent, Emerging Tumors
Authors: Nayak Devanshi A. , Sedlacek Abigail L. , Cillo Anthony R. , Watkins Simon C. , Binder Robert J. 
Published: December 2024

<p>Supplementary figure 15. Separation of gp96-mediated anti-tumor immune function within APC subsets.</p>
Integrating Out-of-Pocket Costs Into Shared Decision-Making for Heart Failure With Reduced Ejection Fraction: A Stepped-Wedge Trial (POCKET-COST-HF)
Authors: Dickert Neal W. , Speight Candace D. , Balser Madeline , Biermann Henry , Davis J. Kelly , Halpern Scott D. , Ko Yi-An , Krishnan Advaita , Matlock Daniel D. , Mitchell Andrea R. , Moore Miranda A. , Montembeau Sarah C. , Morris Alanna A. , Noonan Kathleen , Rao Birju R. , Scherer Laura D. , Sloan Caroline E. , Ubel Peter A. , Allen Larry A. 
Published: December 2024

BACKGROUND:
Guideline-directed medical therapy for heart failure (HF) with reduced ejection fraction can entail high out-of-pocket (OOP) costs, prompting concerns about financial toxicity and access. OOP costs are generally unavailable during encounters. This trial assessed the impact of providing patient-specific OOP costs to patients and clinicians.

METHODS:
This trial was conducted between June 2021 and August 2023 at 6 clinics in 2 health systems using a stepped-wedge, clinic-level cluster-randomized design. Adult patients with HF with reduced ejection fraction (left ventricular ejection fraction ≤40%) were enrolled. The intervention was built upon the EPIC-HF (Electronically Delivered, Patient-Activation Tool for Intensification of Medications for Chronic Heart Failure with Reduced Ejection Fraction) checklist of approved HF with reduced ejection fraction medications. Patients and clinicians received this checklist with (intervention) or without (control) patient-specific OOP cost estimates for higher-cost medications at the time of encounter. Estimates were obtained by providing pharmacy benefit information to a financial navigation firm. Encounters were audio-recorded, and patients were surveyed 2 weeks later. The primary outcome was cost-informed decision-making, defined by mentioning HF medication cost during the encounter. The primary analysis used a generalized linear mixed model. Secondary outcomes were assessed via transcript subcoding and analysis of survey responses.

RESULTS:

Demographic characteristics of 247 patients (mean age, 62.9 years; 29.5% female; 26.3% Black; and 3.2% Hispanic/LatinX) treated by 39 clinicians in intervention and control periods were similar. In the primary model, the rate of cost-informed decision-making was higher in the intervention group than the control group (68% versus 49%;
P
=0.021). Baseline rates of cost discussions and the impact of the intervention varied across sites. When cost discussions were present, fewer discussions in the intervention group involved contingency plans to address potential costs (16.5% versus 31.9%;
P
=0.028). Most other secondary outcomes were not significantly different.

CONCLUSIONS:
Disclosing comprehensive OOP medication costs to patients with HF with reduced ejection fraction increased cost-informed decision-making. Further work is needed to optimize implementation and assess the impact on medication choices and adherence.

REGISTRATION:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT04793880.
The State of Earth Observing System Today: Updates Compiled from Recent EUMETSAT Meteorological Satellite Conferences
Authors: Shenolikar Justin , Ruti Paolo M. , Lee Daniel , Thonipparambil Sreerekha , Tervo Roope , Fierli Federico , Obligis Estelle , Cacciari Alessandra , Raspaud Martin , Bouchet Veronique , Abellan Xavier , McNally Tony , Bessho Kotaro , Boukabara Sid , Brocca Luca , Puca Silvia , Pinardi Nadia , Schulz Joerg , Saalmueller Joachim , Bojkov Bojan 
Published: December 2024

Abstract
This work presents highlights from the 2021 and 2022 EUMETSAT conferences, drawn from their presentations, posters, and panel discussions and placed into the wider context of the global Earth Observing (EO) system. These highlights collectively reveal much about the current state of knowledge about the EO system, its potential future evolutions, and how that system can be used to produce useful products and services. European, American, and Asian space agencies presented their visions for the next generation of operational satellite programs and demonstrated how these will continue to improve environmental forecasting and monitoring products. User communities presented updates on the use of satellite data, including climate records, novel precipitation retrievals, drought monitoring, weather forecasting, and retrievals of a broadening range of trace gases. On the technology side, discussion on the impact of artificial intelligence (AI) on Earth observation was a major theme, particularly for weather forecasting, data assimilation, or other environmental predictions. Cloud computing was another topic due to its potential to streamline the workflow of EO scientists, enhancing collaborations and unlocking access to previously unavailable data or computing resources. Finally, discussion on the miniaturization of observational instruments was another major theme of both conferences, highlighting both the possibility of novel or enhanced observations and the emerging economic case for commercial entities to operate fleets of meteorological satellites.
Relationship between the start time of treatment and patient prognosis in cases of acute wild mushroom poisoning in a certain region of Guizhou Province, China from 2013 to 2020: A retrospective Observational Study and forecast
Authors: Xia Caiyun , Lai Fuping , Wu Jin , Zhan Jiangshan , Zhang Xiaojun , Yu Xian , Liao Ya , Zhang Guiyi , Hu Jie , Wang Tinggang , Lu Yuanlan 
Published: December 2024

Mushroom poisoning has been identified as a primary cause of both foodborne disease outbreaks and related fatalities in China. Depending on the mushroom species ingested, target-organ damage can usually be attributed to the toxins presenting in the mushroom species ingested, which can ultimately lead to organ failure and death. Developing a joint risk prediction model for death caused by acute mushroom poisoning could accurately predict the risk of mortality in such cases, early initiation of bundled treatment could significantly reduce mortality. Methods This single-center, retrospective observational study was conducted on 455 patients with Undergoing Treatment for Mushroom Poisoning at Affiliated Hospital of Zunyi Medical University (AHZMU), the tertiary governmental hospital of China, between January 2013 and December 2020. We investigated the impact of prognostic factors, including the mortality rate of patients who completed treatment at AHZMU versus those transferred to AHZMU, average length of hospital stay, mortality rate for a latency period of > 6-h, major damaged organs, HOPE6-TALK scoring and established a predictive model to assess the severity of acute mushroom poisoning. Results In 2013-2020, there are 455 patients of mushroom poisoning at AHZMU. Mushroom poisonings mainly concentrated in the summer and autumn months, resulted in 47 patients deaths. The first diagnosis cases at AHZMU resulting in a case fatality rate of 12.77% (6/47), Non-first diagnosis patients fatality accounting for 87.23% (41/47). The majority of deaths (89.36%) were attributed to liver injury. Death with incubation period >6-h accounting for 70.21% (33/47) of the total mortality rate. Logistic regression analysis revealed age and HOPE6 scores as independent risk factors, thereby establishing the logistic model equation, an examination via the ROC curve analysis indicates that a combination predictor values (Ycoalition) of 289.6 is the cut-off values for death resulting from acute mushroom poisoning. Conclusion The attending physician should conduct an early HOPE6-TALK scoring and calculate the Ycoalition for patients with acute mushroom poisoning, as well as promptly identify the toxic mushrooms through morphological and molecular biological identification. Identify mushroom species and further infer the clinical type and clinical characteristics. For example, amanitoxion can cause acute liver injury with high mortality. Identify mushroom species that may cause organ damage so that timely implementation of the bundled therapy for poisonous mushrooms will increase the cure rate and reduce the mortality rate (Lu et al., 2019).
The wounded healer: The history and implications of lived experience in mental health care
Authors: Taylor Sarah 
Published: December 2024

Objective The first-hand expertise of people with lived experience has gained prominence in the provision of mental health services over the last 20 years. Unfortunately, due to ongoing stigma, there appears to be an ongoing belief that people with lived experience and mental health professionals are two separate entities. Research suggests otherwise, with a significant proportion of mental health professionals having their own experience of mental crisis or mental illness. Indeed, there are numerous prominent figures, littered through the history of psychiatry, all with lived experience of trauma or mental illness which shaped their contributions to the field. The history and prevalence of lived experience among our clinicians and the benefits brought by their expertise will be discussed in this article. Conclusions Research demonstrates that acknowledging clinicians’ own lived experience has significant benefits in mental health care and outcomes. Perhaps a little more acceptance of our own ‘wounded healers’ is the first step to truly incorporating the wisdom of those with personal lived experience into our mental health services.
Altered platelet phenotype in patients with type II endoleaks following abdominal aortic aneurysm repair
Authors: Pratama Muhammad Yogi , King Benjamin , Teter Katherine A , Bi Christina , Presedo Natalie , Harish Keerthi B , Giardinetto Catarina Santos , Belleca Sheehan , Chervonski Ethan , Maldonado Thomas S , Ramkhelawon Bhama 
Published: December 2024

Background: Endovascular abdominal aortic aneurysm repair (EVAR) is a preferred surgery to prevent aneurysm sac enlargement and minimize the risk of life-threatening rupture in patients with AAA. Serious complications of type II endoleaks following EVAR can cause sac expansion and increase rupture risk. This study focused on evaluating clinical and blood characteristics in patients with type II endoleaks to refine our understanding of systemic fluctuations associated with unsuccessful EVAR. Methods: This retrospective study included 146 patients with AAA who underwent primary elective endovascular procedures (EVAR/fEVAR) between 2013 and 2021. Clinical characteristics, complete blood count (CBC) and imaging data were analyzed from patients who did and did not develop type II endoleaks. Results: Mean platelet volume (MPV) was significantly increased in patients who developed type II endoleaks after EVAR. Receiver operating characteristic analysis showed that MPV has a satisfactory discriminatory performance in distinguishing post-EVAR patients who developed type II endoleaks, yielding an area under the curve (AUC) value of 0.64. A risk stratification panel incorporating MPV, type II diabetes history, and administration of dual antiplatelet therapies yielded an AUC of 0.70 and predicted an endoleak-free survival rate with a hazard ratio of 2.94. A nomogram revealed that MPV had the highest scoring weight among all significant variables. Conclusion: Patients with type II endoleaks following EVAR have elevated MPV indicative of different phenotypes of circulating platelets. MPV presents an attractive predictive criteria for assessing the occurrence of type II endoleaks in patients with AAA.
Validation of the Black-&-White sign to predict intracerebral hematoma expansion in the multi-center PREDICT study cohort
Authors: Pensato Umberto , Tanaka Koji , Ospel Johanna M , Aviv Richard I , Rodriguez-Luna David , Hill Micheal D , Molina Carlos A , Silva Blas Yolanda , Boulanger Jean-Martin , Gord Gubitz , Bhatia Rohit , Padma Vasantha , Roy Jayanta , Dzialowski Imanuel , Kase Carlos S , Kobayashi Adam , Dowlatshahi Dar , Demchuk Andrew M 
Published: December 2024

Background: Hematoma expansion (HE) occurs in one-fourth to one-third of patients with acute intracerebral hemorrhage (ICH) and is associated with worse outcomes. The co-localization of non-contrast computed tomography (NCCT) hypodensity and computed tomography angiography (CTA) spot sign, the so-called Black-&-White (B&W) sign, has been shown to have high predictive accuracy for HE in a single-center cohort. In this analysis, we aimed to validate the predictive accuracy of the B&W sign for HE in a multicenter cohort. Methods: Acute ICH patients from the multicenter, observational PREDICT study (Predicting Hematoma Growth and Outcome in Intracerebral Hemorrhage Using Contrast Bolus CT) were included. Outcomes included HE (⩾6 mL or ⩾33%) and severe HE (⩾12.5 mL or >66%). The association between B&W sign and outcomes was assessed with multivariable regression analyses adjusted for baseline factors. Results: Three hundred four patients were included, with 106 (34.9%) showing HE. The spot sign was present in 76 (25%) patients, the hypodensity sign in 119 (39.1%), and the B&W sign in 29 (9.5%). In the stratum with positive spot signs, patients with B&W signs experienced more frequent HE (79.3% vs 46.8%, p = 0.008), hematoma absolute growth (19.1 mL (interquartile range (IQR) = 6.4–40) vs 3.2 mL (IQR= 0–23.3), p = 0.018), and hematoma relative growth (92% (IQR = 16–151%) vs 24% (IQR= 0–69%), p = 0.038). There was a strong association between B&W sign and HE (adjusted odds ratio (OR) = 7.83 (95% confidence interval (CI) = 2.93–20.91)) and severe HE (adjusted OR = 5.67 (95% CI = 2.41–13.36)). The B&W sign yielded a positive predictive value of 79.3% (IQR = 61.7–90.1) for HE. Inter-rater agreement was moderate (k = 0.54). Conclusion: The B&W sign is associated with an increased likelihood of HE and severe HE by approximately eightfold and fivefold, respectively.
Post-traumatic stress disorder in mother’s of children hospitalized in the neonatal intensive care
Authors: Sellouti Mohamed , Agadr Aomar , Abilkassem Rachid 
Published: December 2024

Background: The progress of neonatal intensive care units has notably enhanced the chances of survival for infants. Despite this, post-traumatic stress disorder (PTSD) in mothers of neonates receiving care in the Neonatal Intensive Care Units (NICU) remains a widely underestimated issue. Aims and objectives: Our objective was to pinpoint the possible risk factors contributing to the development of post-traumatic stress disorder in mothers of neonates receiving care in the NICU. Methods: A prospective, descriptive study was carried out at the Mohamed V Moroccan Military teaching hospital over a 1-year period from January 1st, 2023, to December 31st, 2023, concentrating on neonatal medicine and resuscitation. Mothers were interviewed, and the presence of PTSD after preterm birth and NICU admission was assessed using the Clinician Administered PTSD Scale (CAPS) at 28–30 days following NICU admission or at the time of discharge. Results: The findings revealed that mothers of premature infants displayed more stress symptoms compared to mothers of full-term newborns. Through multivariate analysis, we observed that the gestational age of newborns admitted to the NICU significantly ( p = 0.001) influenced the occurrence of PTSD. Conclusion: Post-traumatic stress disorder in mothers of preterm newborns is a pathological condition that demands proper management from the very first days after birth. The NICU environment remains a significant risk factor for PTSD.