bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Fine Structure of the Comparative
Authors: Caha Pavel , De Clercq Karen , Vanden Wyngaerd Guido 
Published: January 2019

AbstractThe paper provides evidence for a more articulated structure of the comparative as compared with the one in Bobaljik (2012). We propose to split up Bobaljik's cmpr head into two distinct heads, C1 and C2. Looking at Czech, Old Church Slavonic and English, we show that this proposal explains a range of facts about suppletion and allomorphy. A crucial ingredient of our analysis is the claim that adjectival roots are not a‐categorial, but spell out adjectival functional structure. Specifically, we argue that adjectival roots come in various types, differing in the amount of functional structure they spell out. In order to correctly model the competition between roots, we further introduce a Faithfulness Restriction on Cyclic Override, which allows us to dispense with the Elsewhere Principle.
APX001 Pharmacokinetic/Pharmacodynamic Target Determination against
Aspergillus fumigatus
in an
In Vivo
Model of Invasive Pulmonary Aspergillosis
Authors: Zhao Miao , Lepak Alexander J. , Marchillo Karen , Vanhecker Jamie , Sanchez Hiram , Ambrose Paul G. , Andes David R. 
Published: January 2019

APX001, the prodrug of APX001A, is a first-in-class antifungal agent that has a potent activity against
Aspergillus fumigatus
. The goal of current study was to determine the pharmacodynamic (PD) index and target of APX001 in an immunocompromised murine model of invasive pulmonary aspergillosis against 6
A. fumigatus
isolates.
Spinal cord stimulator education during pain fellowship: unmet training needs and factors that impact future practice
Authors: Pak Daniel J , Gruber Jesse , Deer Timothy , Provenzano David , Gulati Amitabh , Xu Yifan , Tangel Virginia , Mehta Neel 
Published: January 2019

Background and objectivesWith a growing need for non-opioid chronic pain treatments, pain physicians should understand the proper utilization of neuromodulation therapies to provide the most comprehensive care. We aimed to identify the unmet training needs that deter physicians from using spinal cord stimulation (SCS) devices.MethodsInternet-based surveys were fielded to fellows enrolled in pain fellowships during the 2016–2017 academic year accredited by the Accreditation Council for Graduate Medical Education and past pain fellows identified through pain medicine societies and SCS manufacturers.ResultsCurrent fellows were more likely to have received SCS training during fellowship compared with past fellows (100.0% vs 84.0%), yet there was variability in fellows’ SCS experiences with a wide range of trials and implants performed. Forty-six percent of current fellows felt there was an unmet training need regarding SCS. Deficiency in SCS case volume was the most common barrier that was noted (38.5%), followed by lack of SCS curriculum (30.8%) and lack of faculty with SCS expertise (23.1%). Lack of training was a predominant reason for past fellows choosing not to use SCS devices postfellowship. The majority of current and past fellows (79.5% and 55.4%, respectively) strongly supported direct training of fellows by SCS manufacturers.ConclusionsWhile SCS training during pain fellowship has become more universal, the experiences that fellows receive are highly variable, and most rely on industry-sponsored programs to supplement training deficiencies. Standardization of SCS procedures may also enable less experienced providers to navigate the SCS treatment algorithm.
Cochlear compound action potentials from high-level tone bursts originate from wide cochlear regions that are offset toward the most sensitive cochlear region
Authors: Lee C. , Guinan J. J. , Rutherford M. A. , Kaf W. A. , Kennedy K. M. , Buchman C. A. , Salt A. N. , Lichtenhan J. T. 
Published: January 2019

Little is known about the spatial origins of auditory nerve (AN) compound action potentials (CAPs) evoked by moderate to intense sounds. We studied the spatial origins of AN CAPs evoked by 2- to 16-kHz tone bursts at several sound levels by slowly injecting kainic acid solution into the cochlear apex of anesthetized guinea pigs. As the solution flowed from apex to base, it sequentially reduced CAP responses from low- to high-frequency cochlear regions. The times at which CAPs were reduced, combined with the cochlear location traversed by the solution at that time, showed the cochlear origin of the removed CAP component. For low-level tone bursts, the CAP origin along the cochlea was centered at the characteristic frequency (CF). As sound level increased, the CAP center shifted basally for low-frequency tone bursts but apically for high-frequency tone bursts. The apical shift was surprising because it is opposite the shift expected from AN tuning curve and basilar membrane motion asymmetries. For almost all high-level tone bursts, CAP spatial origins extended over 2 octaves along the cochlea. Surprisingly, CAPs evoked by high-level low-frequency (including 2 kHz) tone bursts showed little CAP contribution from CF regions ≤ 2 kHz. Our results can be mostly explained by spectral splatter from the tone-burst rise times, excitation in AN tuning-curve “tails,” and asynchronous AN responses to high-level energy ≤ 2 kHz. This is the first time CAP origins have been identified by a spatially specific technique. Our results show the need for revising the interpretation of the cochlear origins of high-level CAPs-ABR wave 1.NEW & NOTEWORTHY Cochlear compound action potentials (CAPs) and auditory brain stem responses (ABRs) are routinely used in laboratories and clinics. They are typically interpreted as arising from the cochlear region tuned to the stimulus frequency. However, as sound level is increased, the cochlear origins of CAPs from tone bursts of all frequencies become very wide and their centers shift toward the most sensitive cochlear region. The standard interpretation of CAPs and ABRs from moderate to intense stimuli needs revision.
2-Deoxy-d-glucose reduces epileptiform activity by presynaptic mechanisms
Authors: Pan Yu-Zhen , Sutula Thomas P. , Rutecki Paul A. 
Published: January 2019

2-Deoxy-d-glucose (2DG), a glucose analog that inhibits glycolysis, has acute and chronic antiepileptic effects. We evaluated 2DG’s acute effects on synaptic and membrane properties of CA3 pyramidal neurons in vitro. 2DG (10 mM) had no effects on spontaneously occurring postsynaptic currents (PSCs) in 3.5 mM extracellular potassium concentration ([K+]o). In 7.5 mM [K+]o, 2DG significantly reduced the frequency of epileptiform bursting and the charge carried by postsynaptic currents (PSCs) with a greater effect on inward excitatory compared with outward inhibitory charge (71% vs. 40%). In 7.5 mM [K+]o and bicuculline, 2DG reduced significantly the excitatory charge by 67% and decreased the frequency but not amplitude of excitatory PSCs between bursts. In 7.5 mM [K+]o, 2DG reduced pharmacologically isolated inhibitory PSC frequency without a change in amplitude. The frequency but not amplitude of inward miniature PSCs was reduced when 2DG was applied in 7.5 mM [K+]o before bath application of TTX, but there was no effect when 2DG was applied after TTX, indicating a use-dependent uptake of 2DG was required for its actions at a presynaptic locus. 2DG did not alter membrane properties of CA3 neurons except for reducing the slow afterhyperpolarization in 3.5 but not 7.5 mM [K+]o. The reduction in frequency of spontaneous and inward miniature PSCs in elevated [K+]o indicates a presynaptic mechanism of action. 2DG effects required use-dependent uptake and suggest an important role for glycolysis in neuronal metabolism and energetics in states of high neural activity as occur during abnormal network synchronization and seizures. NEW & NOTEWORTHY 2-Deoxy-d-glucose (2DG) is a glycolytic inhibitor and suppresses epileptiform activity acutely and has chronic antiepileptic effects. The mechanisms of the acute effects are not well delineated. In this study, we show 2DG suppressed abnormal network epileptiform activity without effecting normal synaptic network activity or membrane properties. The effects appear to be use dependent and have a presynaptic locus of action. Inhibition of glycolysis is a novel presynaptic mechanism to limit abnormal neuronal network activity and seizures.
Sleeping Beauty Insertional Mutagenesis Reveals Important Genetic Drivers of Central Nervous System Embryonal Tumors
Authors: Beckmann Pauline J. , Larson Jon D. , Larsson Alex T. , Ostergaard Jason P. , Wagner Sandra , Rahrmann Eric P. , Shamsan Ghaidan A. , Otto George M. , Williams Rory L. , Wang Jun , Lee Catherine , Tschida Barbara R. , Das Paramita , Dubuc Adrian M. , Moriarity Branden S. , Picard Daniel , Wu Xiaochong , Rodriguez Fausto J. , Rosemarie Quincy , Krebs Ryan D. , Molan Amy M. , Demer Addison M. , Frees Michelle M. , Rizzardi Anthony E. , Schmechel Stephen C. , Eberhart Charles G. , Jenkins Robert B. , Wechsler-Reya Robert J. , Odde David J. , Huang Annie , Taylor Michael D. , Sarver Aaron L. , Largaespada David A. 
Published: January 2019

Abstract

Medulloblastoma and central nervous system primitive neuroectodermal tumors (CNS-PNET) are aggressive, poorly differentiated brain tumors with limited effective therapies. Using Sleeping Beauty (SB) transposon mutagenesis, we identified novel genetic drivers of medulloblastoma and CNS-PNET. Cross-species gene expression analyses classified SB-driven tumors into distinct medulloblastoma and CNS-PNET subgroups, indicating they resemble human Sonic hedgehog and group 3 and 4 medulloblastoma and CNS neuroblastoma with FOXR2 activation. This represents the first genetically induced mouse model of CNS-PNET and a rare model of group 3 and 4 medulloblastoma. We identified several putative proto-oncogenes including Arhgap36, Megf10, and Foxr2. Genetic manipulation of these genes demonstrated a robust impact on tumorigenesis in vitro and in vivo. We also determined that FOXR2 interacts with N-MYC, increases C-MYC protein stability, and activates FAK/SRC signaling. Altogether, our study identified several promising therapeutic targets in medulloblastoma and CNS-PNET.

Significance:
A transposon-induced mouse model identifies several novel genetic drivers and potential therapeutic targets in medulloblastoma and CNS-PNET.
Depression Stresses the Immune Response and Promotes Prostate Cancer Growth
Authors: Mohammadpour Hemn , Bucsek Mark J. , Hylander Bonnie L. , Repasky Elizabeth A. 
Published: January 2019

Abstract
Depression induces secretion of neuropeptide Y from prostate cancer cells, which, in turn, recruits myeloid-derived suppressor cells (MDSC) to the tumor; tumor cells and MDSCs secrete IL6, which activates STAT3 within cancer cells. Prostate cancer samples from depressed patients reveal a similar phenotype, suggesting new treatment strategies based upon blockade of β2-adrenergic receptors and/or neuropeptide Y.
See related article by Cheng et al., p. 2621
EFFECT OF INVASIVE Ageratina adenophora ON SPECIES RICHNESS AND COMPOSITION OF SAPROTROPHIC AND PATHOGENIC SOIL FUNGI
Authors: Balami Sujan , Thapa Lal B. , Jha Sanjay Kumar 
Published: January 2019

Belowground modification of soil microbial community by invasive plants is well evident. Similar instances of Ageratina adenophora invasion have been reported. This study was aimed to determine the effect of A. adenophora invasion on species richness, species or community composition and occurrence frequency of soil fungi. TheseÂ parameters were analyzed using culture method on invaded and uninvaded soils. Species richness of soil fungi wasÂ lower in the A. adenophora invaded soil compared to the uninvaded soil. The occurrence frequency of particular fungiÂ was different for those two soil conditions. A. adenophora also altered soil fungi species composition in the invaded soil by replacing saprophytic fungi and accumulating pathogenic fungi. Thus, A. adenophora is associated to lower speciesÂ richness of saprophytic soil fungi and high occurrence frequency of pathogenic soil fungi. This study concluded that the invasive A. adenophora modifies belowground soil fungi communities as one of the mechanisms involved in theÂ successful invasion of A. adenophora.
PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF MULTISHOOTS DEVELOPMENT IN TRANSGENIC Phalaenopsis amabilis (L.) BLUME HARBORING 35S::KNAT1 (KNOTTED-LIKE Arabidopsis thaliana 1)
Authors: Saputro Triono Bagus , Semiarti Endang , Purwantoro Aziz 
Published: January 2019

Phalaenopsis amabilis (L.) Blume is one of Indonesian natural orchid which has an aesthetic flower and possesses high economic value. The low multiplication rate and long periods of life cycle are the main obstacles to conventionally propagate this orchid. The aims of this research were to analyze the stability of transgenic plant P. amabilis harboring 35S::KNAT1 based on morpho-genomic characterization. KNAT1 gene is reported as a gene that involved in the shoot formation, and it Â had been successfully introduced into Phalaenopsis amabilis (L.) Blume genome. After seven times regeneration, the confirmation of the transgene existence in the genom is needed to ensure whether the plant could consistently maintain the transgene in its genome and to characterize the shoot development. The experiment was carried out in 3 steps:Â 1) Co-integration analysis of 35S::KNAT1 into P. amabilis genom; 2) Phenotypic analysis on the multiplication rate, morphological variation and venation pattern; and 3) Protein profile analysis of transgenic plants. The results showed that the survival rate of putative transgenic was 58.7% on NP0 medium and 62.5% on NP SIM medium. PCR analysis confirmed that 82.5% transgenic growth on NP0 and 93.33% on NP SIM contained DNA fragment of KNAT1 gene, NPTII gene and trnL-F intergenic spacer, indicating that those plants are positive transgenic. The 35S::KNAT1 transgenes and phytohormone were independently involved in multishoots formation of P. amabilis transgenic plants. The phenotypic of plantlets were classified into six main criteria, i.e. normal shape, lobed leaves, rosette, elongated stem, cup shoot and widened leaves. The normal type was the most abundant type of variation (Â± 29%) in both medium. Protein profile showed that all transgenic plants produced 45,8 kDa protein and that was equivalent with molecular weight of KNAT1 protein. Taken together, all those data indicated that 35S::KNAT1 transgene were consistently integrated into the transgenic plant genome.
IDENTIFICATION OF ENDOPHYTIC BACTERIA FROM Curcuma zedoaria BASED ON PROTEIN PROFILE USING MALDI-TOF MASS SPECTROMETRY
Authors: Sulistiyani Tri Ratna , Lisdiyanti Puspita 
Published: January 2019

Valid identification of microorganisms is a vital information to establish culture collections. Currently, molecular approach based on 16S rDNA is widely used for bacterial identification. This approach Â is however, time consuming and expensive. Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) allows the identification of microorganisms directly from colonies and it only takes some few minutes. The interest of this study was to identify endophytic bacteria associated with Curcuma zedoaria based on protein profile using MALDI-TOF MS system and compare with 16S rDNA sequence results. Endophytic bacteria were isolated from part of medicinal plant C. zedoaria collected from Bogor, West Java Indonesia. The identification of selected bacteria was performed by protein profile using MALDI-TOF MS. A total of 66 endophytic bacteria from C. zedoaria plant, were selected for identification. The result of MALDI-TOF MS analysis showed that eleven isolates (16.67%) were correctly identified to the species level and 23 isolates (34.85%) matched on genus level of molecular approach. These results demonstrates that the MALDI-TOF system is suitable and feasible approach for the bacterial identification, mainly for screening and grouping of large number isolates.
Barotropic Instability across the Moat and Inner Eyewall Dissipation: A Numerical Study of Hurricane Wilma (2005)
Authors: Lai Tsz-Kin , Menelaou Konstantinos , Yau M. K. 
Published: January 2019

Abstract
Radar imagery of some double-eyewall tropical cyclones shows that the inner eyewalls become elliptical prior to their dissipation. These elliptical features indicate that the barotropic instability (BI) across the moat (aka, type-2 BI) may play a role in the process. To investigate the mechanism for dissipation, a WRF simulation of Hurricane Wilma (2005) is performed. The results reveal an elliptical elongation of the inner eyewall and a change in the structure of the radial flow from wavenumber (WN) 1 to WN 2 at the lower levels. A linear stability analysis as well as idealized nonlinear experiments using a nondivergent barotropic vorticity model initialized with the vorticity fields before the change in the dominant wavenumber of the radial flow are presented with the results supporting the presence of a type-2 BI at the lower levels. The accompanying WN-2 radial flow is also found to dilute the vorticity within the inner eyewall and the eye. However, this dilution is not seen at higher levels as the type-2 BI becomes weak and short lived at the middle levels and reaches its weakest strength at the upper levels. This phenomenon is traced to the fact that a higher growth rate comes with a narrower moat for type-2 BI. As the outward slope of the outer eyewall is larger than that of the inner eyewall, the moat width increases with height so that the growth rate decreases with height. The results presented here thus highlight the potential role played by the barotropic instability across the moat in inner eyewall dissipation.
Using Atmospheric Energy Transport to Quantitatively Constrain South Pacific Convergence Zone Shifts during ENSO
Authors: Lintner Benjamin R. , Boos William R. 
Published: January 2019

AbstractThe South Pacific convergence zone (SPCZ) exhibits well-known spatial displacements in response to anomalous sea surface temperatures (SSTs) associated with the El Niño–Southern Oscillation (ENSO). Although dynamic and thermodynamic changes during ENSO events are consistent with observed SPCZ shifts, explanations for these displacements have been largely qualitative. This study applies a theoretical framework based on generalizing arguments about the relationship between the zonal-mean intertropical convergence zone (ITCZ) and atmospheric energy transport (AET) to 2D, permitting quantification of SPCZ displacements during ENSO. Using either resolved atmospheric energy fluxes or estimates of column-integrated moist energy sources, this framework predicts well the observed SPCZ shifts during ENSO, at least when anomalous ENSO-region SSTs are relatively small. In large-amplitude ENSO events, such as the 1997/98 El Niño, the framework breaks down because of the large change in SPCZ precipitation intensity. The AET framework permits decomposition of the ENSO forcing into various components, such as column radiative heating versus surface turbulent fluxes, and local versus remote contributions. Column energy source anomalies in the equatorial central and eastern Pacific dominate the SPCZ shift. Furthermore, although the radiative flux anomaly is larger than the surface turbulent flux anomaly in the SPCZ region, the radiative flux anomaly, which can be viewed as a feedback on the ENSO forcing, accounts for slightly less than half of SPCZ precipitation anomalies during ENSO. This study also introduces an idealized analytical model used to illustrate AET anomalies during ENSO and to obtain a scaling for the SPCZ response to an anomalous equatorial energy source.
Numerical Evaluation of Energy Transfers in Internal Gravity Wave Spectra of the Ocean
Authors: Eden Carsten , Pollmann Friederike , Olbers Dirk 
Published: January 2019

AbstractSpectral energy transfers by internal gravity wave–wave interactions for given empirical energy spectra are evaluated numerically from the kinetic equation that is derived from the assumption of weak interactions. Wave spectrum parameters, such as bandwidth, spectral slope, and Coriolis frequency f, are varied, as is the spectral resolution. In agreement with previous studies, we find in all cases a forward energy cascade toward smaller vertical and horizontal wavelengths. Energy sinks due to the transfers are predominantly at frequencies between 2f and 3f. While the mechanism of the energy transfer differs partly from findings of previous studies, a parameterization for internal wave dissipation—which is used in the fine structure parameterization to estimate dissipation and mixing rates from observations—agrees well with the numerical evaluation of the energy transfers. We also find a dependency of the energy transfers on the spectral slope, offering the possibility to decrease the bias of the fine structure parameterization by improving the knowledge about the spatial variations of this (and other) spectral parameter.
Commonwealth Digest
Authors: Bradshaw J.T. , Meagher M.V. , Buchanan P. , Thynne J.T. 
Published: January 2019

This digest covers the period 26 March 1963 to 30 October 1963, and is compiled from the published debates of the Parliament of the Commonwealth of Australia.The page references to Parliamentary Debates (‘S. Deb. ’ and ‘H.R. Deb.’) are to the published debates of the Senate and the House of Representatives, for the first Session of the Twenty-fourth Parliament, third and fourth periods.
Total Wage — An Analysis
Authors: Hawke R. J. 
Published: January 2019

If it is in any sense true that the establishment of the federal arbitration system created “a new province for law and order” as Higgins asserted, then it is certainly true that the concept of the basic wage as a foundational element in all awards of that system made in settlement of interstate industrial disputes became the single most important factor in the development of that law and of that order. From the Harvester judgment of 1907 emerged the structure of:
(i)a basic wage common to all workers irrespective of the work upon which, or the industry in which, they were employed and(ii)a secondary wage or margin for the skill, responsibility, or particular circumstances of the work, or industry, in questionwhich was the distinguishing feature of wage determination in Australia for the next sixty years. All federal and State industrial tribunals operated with in this structural framework, either by legislative direction, or as a result of their own deliberate decisions based upon a common acceptance of this structure by the parties coming before these tribunals.