bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polianthes tuberose Breeding in Indonesia
Authors: Sihombing Donald 
Published: March 2018

Tuberose (Polianthus tuberosa) is an important ornamental plant in Indonesia that has been cultivated by farmers for a long time mainly in Java and North Sumatra provinces. However, the availability of new superior varieties is only a few so that the consumers only have a limited choice. Until now, there are three types of tuberose in Indonesia, which is distinguished by the type of flower namely single, semi-double and double flower. Some breeding efforts to obtain new varieties have been done through crossbreeding, mutation induction and selection to local cultivars. But the success on the crossbreeding of tuberose was only about 0.05%, as it can only be done in the direction of the single flower with double flowers. Because in the double flowers pistils are not found, the variability is very low. Nevertheless from these crossbreeding obtained 29 genotypes that showed differences in several characters, especially in the arrangements of flowers; and flower stalks straightness and rigidness. The other study indicates that each genotype has a different resistance levels against leaf spot disease (Xanthomonas sP.). Meanwhile, breeding of tuberose through mutation induction technique has not been widely studied. Application of gamma ray irradiation on tubers caused morphological damaged on Polyanthes, reduced bulb growth less than 30% and reduced the plant height more 400% than no treated plants (control). Polyploidy induced mutations by using colchicine led to changes in the length and diameter of the flowers and harvesting time. Until now, there is no result of the crossbreeding and mutation techniques that released as a new variety. Despite this, based on the positive selection breeding on local cultivars, there have been two superior genotypes released as new varieties namely Dian Arum (double flower type; origin of Cianjur, West Java) and Roro (semi double flower type; origin of Pasuruan, East Java). Keywords: Polianthes tuberosa ; breeding; local cultivars, new varieties,Dian Arum, Roro Anteng
Agrobacterium tumefaciens-mediated Transformation of Embryogenic Callus and Somatic Embryos of the Banana cv “Ambon Lumut” (Musa acuminata)
Authors: Kusumastuti Tifa R. , Esyantia Rizkita R. , Dwivany Fenny M. 
Published: March 2018

Banana is one of the major fruit crops, though its conventional breeding has limitations, such as sterility and high polyploidy levels. Biotechnological approach using genetic transformation crop for improvement offers an alternative solution. In this study a protocol was developed for establishing genetic transformation from embryogenic callus and somatic embryos of the banana cv Ambon Lumut . Embryogenic callus was obtained in ID4 medium (MS-based medium) supplemented with 1 mg L-1 IAA, 4 mg L-1 2,4D, and 0.03 g L-1 active charcoal. Embryogenic callus was transferred into liquid mediu m to establish somatic embryos. Embryogenic callus and somatic embryos were used for Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain A GL1, containing pART-TEST7 p lasmid with gfp gene as a reporter and CaM V35S as a promoter, was used for transformations. The embryogenic callus and somatic embryos were transformed using heat-shock method followed by centrifugation (2000 rpm) and co-cult ivation in liquid medium containing acetosyringone (100 M) for 3 days. Results of the GFP analysis showed transient expression from gfp gene reporter in transformed embryogenic callus and somatic embryos. Transformation efficiency in somatic embryos (85,9%) was higher than that in embryogenic callus (32.09%). PCR analysis using CaMV primer showed bands that compatible with CaMV35S promoter at 507 bp. This is a report showing establisment of embryogenic callus and somatic embryo culture transformation by using A. tumefaciens-mediated transformation protocol of the local banana cv Ambon Lumut. This study proved the huge potential for genetic transformation of banana cv Ambon Lumut for crop improvement, such as pest or disease resistance and abiotic factor stress tolerance. Keywords: banana; embryogenic callus; somatic embryos.
Performance of New Rice Variety (Inpari 24) at Subak Amerta Nadi Kerta Village Payangan Sub-district of Gianyar Bali
Authors: Kariada I Ketut , Aribawa I. B. 
Published: March 2018

Model development of rice farming in the production centre area at Payangan Sub-district of Gianyar is designed to introduce a model called rice field laboratory. The principle of the model used is "build, operate and transfer (BOT), refering to a the disseminated model of innovation which is a large scale pilot model at the grass root level. The study introduced new varieties “Inpari”24 as an attempt to replace the old varieties that has lower productivity. Cultivation technology by implementing Legowo System 2: 1. The aim of this study was to determine the performance of new variety Inpari 24 in the cropping system in Subak Amerta Nadi, Kerta Village, Sub-district of Payangan Gianyar in the growing season 2014. This study used a randomized block design with two treatments of different cropping systems, namely: Legowo 2: 1 (40 x 20 x 10 cm) and tiles (20 x 20 cm), repeated 10 times. Variables observed in this study were: plant height, number of tillers, panicle length, number of grains per panicle and empty grains and yield per hectare. Data were analysed through analysis of variance, then followed by LSD test at 5% level. It was concluded that: (a) cropping systems significantly affected all variables of rice production, (b) the highest productivity was Legowo System which was 7.50 tons / ha, increased by 26.68% compared to tiles cropping system (the method used by farmers).Keywords: Inpari 24; planting system; rice
Grafting of Tomato with Eggplant Rootstock at Penyabangan Village Payangan Subdistrict of Gianyar Bali
Authors: Kariada I. Ketut , Aribawa I. B. 
Published: March 2018

Grafting on tomatoes is performed to obtain tomato plants that are resistant to bacterial wilt, waterlogging resistant, resistant to fusarium wilt and nematodes. Bacterial wilt caused by Ralstonia solanacearum causes the tomatoes grown in the rainy season to wilt and die. These bacteria live in the soil and invade plants through roots. To overcome this disease, grafting was carried out by using resistant eggplant as rootstock. This method is expected to reduce the attack of bacterial wilt diseases on tomato plants. The study was conducted in the village of Penyabangan Payangan Gianyar in FY 2014. This area was located at the plateau of AEZ wet climate. The experimental design used was a randomized block design (RBD) with two treatments and 10 replications. Materials used were tomato plants that have been joined with eggplant rootstock (grafted) and tomato plants without root stock (non-grafted) for comparison. The varieties used were varieties F1 Marta. In field applications, the scion was prevented from touching the ground while the rootstock was fully embedded into the ground. Based on the plant height, there was a slower growth of the grafted tomato plants compared with that of non-grafted one in the early stages of growth but became normal in generative phase. It was also found that the yield of the grafted tomato was lower (2.54 kg. tree-1) than that of the non-grafted one (3.19 kg.tree-1). Keywords: grafting; tomatoes; eggplant; stem rot; bacterial wilt (alphabetic in order).
Monitoring of Artificial Nests in Horticultural Ecosystems-Observation of Woodpeckers Ethology
Authors: C.A. Mihai , F. Stănică , M.R. Ionescu 
Published: March 2018

In order to increase the integrated protection of horticultural ecosystems by using the biological fight, the installation on artificial nests, is an important measure to help the insectivore birds. In a research program, a number of 166 artificial nets for Passeriformes birds were installed in different ecosystems as parks, botanical gardens and orchards in the South-Eastern part of Romania. During the nests monitoring period an interesting, but in the same time warring phenomenon, was noticed: a certain number of artificial nets were prayed by woodpeckers, especially by Dendrocopos major (Great Spotted Woodpecker). In Romania, all the ten European woodpecker species are nesting, nine being sedentary and one (Jynx torquilla) migratory, leaving the country in autumn. Woodpeckers in general, are very useful in woody plant biological protection, having a predominant insectivore nutrition and being the only one to keep under control the insects that are leaving on, inside and under the trees bark. The paper presents some data regarding the woodpeckers ethology in the studied ecosystems. More research are needed to understand the woodpeckers behaviour and to find protection methods of useful birds' nests against the woodpeckers attack. Keywords: useful birds; Dendrocopos major; Dendrocopos syriacus; Passer montanus; Picus viridis.
Recent Status of Pests and Diseases on Cut Roses in Batu East Java
Authors: Handayati Wahyu , Sihombing Donald 
Published: March 2018

Rose is one of the most important ornamental plants in Indonesia. Almost 57.02% of the national cut rose’s demand was supplied from East Java, mainly from Batu. However, in the production process, there are various problems, especially pest and disease damages. In this regard, a study was conducted to determine recent status of the pests and diseases on roses in cultivation center of Batu, East Java. Observation was carried out in the farmers’ field randomly, from October to December 2014. Observation was performed on type and damage intensity of the pests and diseases. The pests and plant damage were sampled for further identification. An interview was made with some farmers to get secondary data about the type and the importance of the pests and diseases as well as the effort for controlling them. The result showed that some pests and diseases found included aphid, armored scale insect, spider mite, thrips, black spot, crown gall and powdery mildew. Based on morphological identification, there were two families of armored scale insect, i.e., Coccidae and Diaspidae. The highest damage intensity was due to armored scale insect, thrips, black spot and crown gall with the percentage of damage intensities of 50.50%, 20.00%, 45.50% and 65.00%, respectively. Based on those data, scale insect, thrips, black spot and crown gall were classified as important pests and diseases on roses in this area. Some pesticides and other materials like petroleum oil and detergent were used by the farmers to control either pests or diseases, but they were ineffective, especially in controlling armored scale insect and crown gall. Keywords: Cut roses, pest, disease, inventory, observation, status
Bio-ecology of Slender Black Rice Bug, Paraeucosmetus pallicornis in South Sulawesi
Authors: Rahmini Rahmini , Munawar Dede , Senoaji Wasis , Baliadi Yuliantoro 
Published: March 2018

A study on the bio-ecology of slender black rice bug, Paraeucosmetus pallicornis, was conducted in the research farm of Lolit Tungro, Lanrang, South Sulawesi, Indonesia. This pest is considered as new rice pest, attacking rice plant especially during generative stage. This pest inserts its stylet and then sucks the sap of the developing rice grain. Light trap was used to catch this pest. Yellow sticky trap and pitfall trap were used to determine the insect population and to find out when the pest infests the plant. Fifteen yellow sticky traps were set diagonally on rice field, and 10 pitfall traps were placed on the ground. The traps were placed on three plots as replication. On the first week of the study, it was found that the number of captured insects from the light trap during harvesting was 193. On the 2nd to 4th weeks, during fallow stubble, the captured insects were 135 -740. In the early of May, the field started to be ploughed as preparation for the next planting season. As the result, the number of insects captured decreased to 53 – 152 insects. The 2013 planting season was started in June. During this period, the bugs captured were only 1 – 3. This indicates that the bugs have already moved or migrated out of the rice field. The average number of eggs laid were 53.3 (1 pair), 124.8 (2 pairs), 142.5 (3 pairs), 202.3 (4 pairs), and 284 (5 pairs) and the average of hatch rate was 29.9%. The damaged rice grain was 38% grains/panicle (ranged 24.2-57.4%). This level of damage indicates that the P. pallicornis contributes to the reduction of rice yield. Keywords: Paraeucosmetus pallicornis, rice pest
Assessment of Food Security Determinants Among Rice Farming Households in Bali Province
Authors: Suharyanto Suharyanto , Indrasti Rita 
Published: March 2018

This study aimed to investigate food security at household level and to identify the socio-economic factors that affect the levels of food security among households of lowland rice farmers. Research was conducted in three district centers of rice production in the province of Bali, i.e. Buleleng, Gianyar and Tabanan. Samples were taken by multistage sampling of 216 respondents consisting of 122 farmers ICM program and 94 farmers non-ICM program. The data for the study were obtained with the aid of a structured questionnaire survey randomly administered to rural farming households in the districts. Household food security was measured by cross classification of the share of expenditure on food and consumption of energy. The socio-economic factors that affected household food security levels were estimated using ordered logistic regression. The result showed that in the aggregate 49.07% of the households were categorized as secure, 37.9% as vulnerable, 8.79% as insufficient, and 4.17% as insecure. The analysis showed that housewives education, incomes and household food reserves had a significant positive effect on the level of food security whereas family size, the prices of rice and instant noodles had a significant negative effect. The food security level of the ICM-program farmers was higher than that of the non-ICM program farmers Keywords : food security; households; lowland rice; integrated crop management (ICM)
Knowledge, Attitude and Behaviour of Farmer on Integrated Crop Management for Healthy Citrus Orchard (ICMHCO) in Gianyar District
Authors: Suharyanto Suharyanto , Arya Nyoman Ngurah , Sutami Putu 
Published: March 2018

One cause of low productivity and quality of citrus in Indonesia is not free of CVPD (Citrus Vein Phloem Degeneration) or Huang Lung Bin (HLB) disease in several citrus production centers. Integrated Crop Management for Healthy Citrus Orchard (ICMHCO) is a HLB control strategy consisted of (a) the used of labeled and free diseases planting materials, (b) pest and diseases control especially for the HLB vector, (c) good field sanitation, (d) optimum cultural practices, and (e) field management consolidation. The research objective was to determine the knowledge, attitudes and behavior of farmers in the control HLB. The research was conducted in the village of Pupuan, Tegallalang Sub-district, Gianyar District on August 2015, using a survey of 30 farmers of respondents. Data were analyzed descriptively uses the cross tabulation. The results showed that the knowledge of farmers on ICMHCO could be classified as moderate, relatively neutral attitude of farmers and farmers' behavior is low. Efforts should be made to improve the implementation of ICMHCO, among others, by increasing the availability of seed labeled and free of disease and other production inputs, training and institutional capacity building of farmer groups. Keywords: knowledge; attitude; behavior; citrus farmer
The Effect of CMC Addition on the Characteristics of Sweet Potato (Ipomoea Batatas L. Cv Cilembu) Velva
Authors: Djali Mohamad , Firbiani Maulisa , Marsetio Marsetio 
Published: March 2018

Indonesia is the fourth largest sweet potato producing country in the world. However, the number of sweet potato utilization is still non optimal. One of the most popular sweet potato cultivars is Cilembu sweet potato. Velva is a frozen dessert which is made from mashed fruit (puree) with addition of water and sucrose. Fat content in velva is much lower than the ice cream, it is suitable to be consumed by vegetarian and those who are on low-fat diet. The velva making process may serve as an alternative of sweet potato utilization and will increase its economic value. One of the factors that affects the texture of velva is the type and amount of stabilizers used. This research aims to determine the optimal amount of Carboxy Methyl Cellulose(CMC) to yield Cilembu sweet potato velva with good characteristics and organoleptic properties of the panelists preferences. The method used is the experimental method with completely randomized design (CRD) consisted of 6 treatments of CMC addition (0.00%, 0.20%, 0.40%, 0.60%, 0.80%, and 1.00%) and replicated 4 times. The final results showed that the sweet potato velva with addition of 0.60% CMC was the best treatment. The velva has rate of melting 712.50 seconds, overrun 19.69 %, and the amount of total soluble solids 27obrix. This velva has qualified the standard of rate of melting, according to Arbuckle and Marshall (1996) the ice cream’s rate of melting in 20oC is 10-15 minutes. Keywords: velva; sweet potato; CMC; characteristics
Functional and Amylographic Properties of Physically-Modified Sweet Potato Starch
Authors: Marta Herlina , Tensiska Tensiska 
Published: March 2018

In general, native sweet potato starch has inferior characteristics such as it swells easily, does not gel firmly and low paste clarity. The characteristics of native sweet potato starch cause limitation in its utilization. This research aimed to study the effect of physically modified starch on the functional and amylographic properties of native sweet potato starch. The study used a descriptive method with 4 treatments and 2 replications: a) a native sweet potato starch, b) sweet potato modified starch by heat moisture treatment, c) sweet potato starch modified by annealing and d) sweet potato starch modified by pre-gelatinization. The results showed that all three treatments modified starches largely alter the functional and amylographic properties of native sweet potato starch. Heat moisture treated and annealed sweet potato had starches with decreased swelling volume, solubility, peak viscosity, and breakdown viscosity, increased pasting temperature and setback viscosity than its native starch. Pre-gelatinized sweet potato starch has lower bulk density, peak viscosity, breakdown viscosity, setback viscosity and increased swelling volume, solubility and water absorption capacity than its native starch. Key word: functional properties; amylographic properties; sweet potato starch; physically modified
Urban Agriculture Development : A Strategy to Support Food Security
Authors: Juniawati Juniawati , Hayuningtyas Maulida 
Published: March 2018

The enhancement of rural-urban migration flows impacts on the enhancement of urban population. It takes more efforts to fulfill the food demand. Now, 80% food stock in urban area is fulfilled from rural area and imports. Urban agriculture development is a strategy to improve food availability, food access and also to support food security. Some of urban agriculture models that have implemented and developed in many country were metropolitan food cluster, rooftop garden, community garden, and vertical farming. This paper is a literature review from various study, research and document relates with urban agriculture. Based on this study, urban agriculture can increase urban community prosperity, environmental sustainability and quality of health. Urban agriculture productivity needs agricultural technology innovation to ensure sustainability of production. Urban agricultural models that is integrated with agricultural technology innovation able to create a resilient urban food systems to accelerate the realization of food security. Keywords: urban agriculture; technological innovaton; food security
Toll-Like Receptors (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Proteins
Authors: Lastuti Nunuk Dyah Retno , Rantam Fedik Abdul , Hastutiek Poedji , Chrismanto Dony 
Published: March 2018

Sarcoptic mange is one of the most economically important diseases in goats in Indonesia, and increasing number of cases of treatment failure is being reported because of drug resistance. Nowadays, it is considered as an emerging/re-emerging parasitic disease that threatens human and animal health globally. Toll-Like Receptors (TLR) is a receptor which plays role in innate immunity due to microbial infection. TLR-5 is a receptor that can recognize ligand produced by bacterial component, and TLR-7 is involved in the recognition of ligand that similar to ssRNA virus. This research aims are to detect Sarcoptes scabiei antigenic protein which can induce cellular immune response in rabbit as adaptive immunity with TLR-5 and TLR-7 as marker.This research was performed in several stages: isolation and identification S. scabiei from scabies infected goats; extraction of soluble protein S.scabiei mite, the rabbit immunization by inoculating protein antigen S. scabiei with dosage of 500µg, repeated five times as booster in two weeks, the examination of TLR-5 and TLR-7 expression using direct immunofluorescence technique. The result of cellular immune response is shown by TLR-5 and TLR-7 expression in rabbit T limphocytes which appear yellow to green fluorescence color using fluorescence microscope. The amount of fluoresnce T lymphocytes showed a significant difference (p<0.05) between control and various boosters, and significantly increased in 3rd booster or 42 days post immunization. The conclusion showed protein from S. scabiei mites taken from goat contains ligands, acting as receptors which involve in pathogen associated molecular pattern (PAMP) that can induce adaptive immunity and recognized by TLR-5 and TLR-7. It shows that TLR is not only involved in innate immunity but also in adaptive immunity, and can be used as alternative adjuvant development. Keywords: Sarcoptes scabiei, TLR- 5, TLR-7, goats, rabbits.
Preservation Effect of Grouper (Epinephelus sp) Fillet Against Survival of Anisakidae
Authors: Raharjo Hartanto M. , Koesdarto Setiawan , Estoepangestie A.T Soelih , L.D Kusuma Wardhani 
Published: March 2018

This study was aimed to determine the effect of time and preservation method that the most effective in killing larvae Anisakidae, and still be able to maintain the quality post-preservation of fillet from three groups, combination of pickling with heating (50°C and 75°C ); pickling with cooling (ice and dry ice); and pickling with salting (dry salting and brine salting). This study was a laboratory experimental research carried out in four phases, phase identification, preservation, organoleptic test and protein test. The results can be concluded that the time of preservation and preservation methods affect the survival of anisakidae larvae. The most effective heating method preservation to kill the larvae Anisakidae was heating at temperature of 75°C compared to 50°C, for cooling the use of dry ice is more effective than ice, and for methods of salting with 25% concentration, brine salting and dry salting effective in killing larvae Anisakidae , The effect of the method preservation for the quality of fillets in organoleptic test still considered adequate and still can be accepted by the public. As for the protein test a slight decline of the fillet quality post-preservation. Keyword : Preservation; Fillet of Grouper; Larva Anisakidae