bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collaborating With Teacher Librarians to Support Adolescents’ Literacy and Literature Learning
Authors: Merga Margaret Kristin 
Published: February 2019

AbstractThe author explores teacher librarians’ ideas about teacher collaborations around literacy and literature learning, roles in collaborations, and the characteristics of good collaborations. Productive and strong collaborations between librarians in schools and their teacher colleagues can enhance students’ learning experiences and outcomes. Semistructured interview data were collected from teacher librarians at 25 schools. The author reports on teacher librarians’ identification of a range of barriers to collaboration in contemporary literacy‐learning contexts and explores the multiple roles that teacher librarians may adopt in collaborative relationships, identifying 11 characteristics of strong collaborations as identified by teacher librarians, which encompass diverse interpersonal qualities and professional activities. Findings offer ideas to enhance the quality of teacher–librarian collaboration, improving literacy outcomes for adolescent students.
microRNA‐510‐5p promotes thyroid cancer cell proliferation, migration, and invasion through suppressing SNHG15
Authors: Liu Yongcun , Li Junli , Li Meng , Li Feng , Shao Yuan , Wu Liping 
Published: February 2019

AbstractSNHG15 has been suggested to be correlated with clinical progression and prognosis, and function as tumor suppressive long noncoding RNA in thyroid cancer at our previous study. SNHG15 was proposed to be a potential target for miR‐510‐5p at LncBase Predicted database. Thus, the aim of this study was to explore the relationship between miR‐510‐5p and SNHG15 in thyroid cancer, and the clinical significance of miR‐510‐5p in patients with thyroid cancer. In our results, levels of miR‐510‐5p expression were increased in thyroid cancer tissues and cell lines compared with adjacent normal thyroid tissues and normal thyroid cell line, respectively. There was a statistically negative correlation between SNHG15 expression and miR‐510‐5p expression in thyroid cancer tissues. Moreover, miR‐510‐5p directly bound to SNHG15, and negatively regulated SNHG15 expression in thyroid cancer cells. Furthermore, miR‐510‐5p promoted thyroid cancer cell proliferation, migration, and invasion through suppressing SNHG15. Finally, high miR‐510‐5p expression was observed in tumor tissues with advanced clinical stage or lymph node metastasis. In conclusion, we provide evidence to support a pivotal role for miR‐510‐5p in regulating thyroid cancer cell proliferation, migration, and invasion.
Introduction of cell‐free DNA screening is associated with changes in prenatal genetic counseling indications
Authors: Stevens Blair K. , Noblin Sarah J. , Chen Han‐Yang , Czerwinski Jennifer , Friel Lara A. , Wagner Chelsea 
Published: February 2019

AbstractThe introduction of cell‐free DNA screening, or non‐invasive prenatal testing (NIPT), for chromosome abnormalities has greatly impacted prenatal care since its introduction in late 2011. We aimed to evaluate the association between the introduction of cell‐free DNA screening and indication and referral patterns for genetic counseling at a large US academic medical center by comparing the percentage of each counseling indication between the time period prior to the introduction of cell‐free DNA screening (2006–2011) and following its introduction (2012–2016) using multivariable Poisson regression models. Genetic counseling indications for positive carrier screens, average risk patients, abnormal ultrasound findings, and family history indications were significantly higher following the introduction of NIPT while advanced maternal age and abnormal maternal serum screening indications dropped significantly. We also showed that the uptake of amniocentesis dropped significantly after the introduction of cell‐free DNA screening, while chorionic villus sampling uptake increased. These results provide evidence that the introduction of new genetic screening technologies is associated with a shift in genetic counseling referral indications and an increased uptake in genetic screening. Additional research is needed to explore the impact of expanded testing options on the need for genetic counseling services.
Aqueous extracts of Syzygium brazzavillense can inhibit the infection with coxsackievirus B4 in vitro
Authors: Badia‐Boungou Francis , Sane Famara , Alidjinou Enagnon Kazali , Hennebelle Thierry , Roumy Vincent , Ngakegni‐Limbili Adolphe Christian , Nguimbi Etienne , Moukassa Donatien , Abena Ange Antoine , Hober Didier 
Published: February 2019

AbstractTraditional practitioners commonly use plant crude extracts to treat various diseases in patients with symptoms that can be seen during enterovirus infections. In this study, the antienteroviral activity of medicinal plants from the Republic of Congo has been evaluated in vitro. Through an ethnopharmacological approach, seven plants grouped into six families were identified. Aqueous and organic extracts of various organs from these plants were prepared. The organic extracts at subcytotoxic concentrations did not inhibit the cytopathic effect (CPE) induced by coxsackievirus (CV)B1‐5, CVA6, poliovirus type 1, and enterovirus 71. The aqueous extract of Syzygium brazzavillense, but not those of other plants, inhibited the CPE induced by CVB3 and CVB4 at 30 µg/mL (CC50; 2800 µg/mL, IC50; 0.8 µg/mL) and by CVB2 and poliovirus type 1 at higher concentrations. When aqueous extract of this plant was mixed with CVB4, the replication of the virus was inhibited. In conclusion, aqueous extracts of Syzygium brazzavillense can inhibit the infection with CVB4 and other enteroviruses in vitro. The present ethnopharmacological investigation helped to identify a plant with potential properties useful to combat enterovirus infections.
On/off resonance Raman optical activity of human serum transferrin
Authors: Bogaerts Jonathan , Johannessen Christian 
Published: February 2019

AbstractThe transferrin proteins exhibit two high affinity Fe(III) binding pockets and are responsible for the iron transport into cells of higher order organisms. Previously, transferrins have been studied as possible transporter molecules for drugs delivery. In this study, Raman optical activity (ROA) was employed to investigate human serum transferrin. Due to the presence of Fe(III) in the holo form of the protein, it is in resonance with the the laser excitation wavelength (of 532 nm) used in the experiments. Consequently, resonance enhanced ROA bands were observed in the spectrum of the holo form. Nevertheless, far from resonance bands, arising from the protein backbone, could simultaneously be observed in the ROA spectrum of the holo form. This implies that ROA can be used to provide simultaneously information on the metal binding pocket as well as on the secondary structure of the protein. Furthermore, it was found that the amount of observed resonance enhanced ROA signals can be tuned by partially removing the iron present in the protein. Electronic circular dichroism (ECD) was employed to verify the Raman/ROA results.
New sights into lipid metabolism regulation by low temperature in harvested Torreya grandis nuts
Authors: Zhou Minying , Song Lili , Ye Shan , Zeng Wei , Hännien Heikki , Yu Weiwu , Suo Jinwei , Hu Yuanyuan , Wu Jiasheng 
Published: February 2019

AbstractBACKGROUNDTorreya grandis, a large evergreen coniferous tree with oil‐rich nuts, undergoes a crucial ripening stage after harvest that results in oil accumulation, finally giving rise to the nut that is edible in roasted form. To understand lipid metabolism in T. grandis nuts during the post‐harvest ripening period, the effects of low temperature on lipid content, fatty acid composition, lipid biosynthesis and degradation were investigated.RESULTSThe lipid content increased during ripening at room temperature and a low temperature slowed down this increase. Linoleic acid content increased at low temperature, which was accompanied by an increase in the microsomal oleate desaturase (FAD2) activity and FAD2 expression. Furthermore, a low temperature attenuated lipid peroxidation as indicated by lower contents of malondialdehyde, hydroperoxide and total free fatty acid in T. grandis nuts during the ripening stage, as well as the down‐regulation of gene expression of lipid degradation‐related enzymes such as phospholipase D and lipoxygenases.CONCLUSIONThe findings of the present study indicate that a low temperature increased polyunsaturated fatty acid contents by increasing FAD2 biosynthesis and decreasing lipid peroxidation, thereby improving the oil yield in T. grandis nuts during the post‐harvest ripening period. © 2019 Society of Chemical Industry
Spatial regularity of semigroups generated by Lévy type operators
Authors: Liang Mingjie , Wang Jian 
Published: February 2019

AbstractWe apply the probabilistic coupling approach to establish spatial regularity of semigroups associated with Lévy type operators, by assuming that the corresponding martingale problem is well posed. In particular, we can prove the Lipschitz continuity of the associated semigroups, when the coefficients are Hölder continuous but the corresponding Lévy kernel may be singular.
Targeted next generation sequencing identified novel loss‐of‐function mutations in MERTK gene in Chinese patients with retinitis pigmentosa
Authors: Liu Song , Bi Jian Gang , Hu Yunlong , Tang Donge , Li Bo , Zhu Peng , Peng Wujian , Du Dong , He Huiyan , Zeng Jun , Dai Yong 
Published: February 2019

AbstractBackgroundRetinitis pigmentosa (RP) is one of the major types of hereditary retinal dystrophies with extreme genotypic heterogeneity. To date, more than 80 genes have been identified to be associated with RP in human.MethodHere, we presented a clinical genetic study of three Chinese man manifested with night vision blindness and complete loss of midperipheral visual field. All of these three probands have been identified with loss of both central vision and far peripheral visual field. Gradual loss of rod cells followed by subsequent loss of cone cells have been identified in these probands. Targeted next generation sequencing and Sanger sequencing have been performed to understand the pathogenic variants underlying the disease phenotype in these three unrelated Chinese probands.ResultsTargeted next generation sequencing and Sanger sequencing identified three homozygous novel mutations (c.1880C>A; c.1459_1460delGA, and c.392G>A) in the MERTK gene in these three unrelated Chinese proband. In the first proband, the identified mutation (c.1880C>A) leads to the formation of a premature stop codon followed by the formation of a truncated mer‐tyrosine kinase (MERTK) protein (p.Ser627*) product which predicted to be disease causing. In the second proband, the identified deletion (c.1459_1460delGA) leads to the formation of a frameshift which also finally results in the formation of a truncated MERTK protein (p.Asp487Leufs*57) product which also predicted to be disease causing. In the third proband, the identified mutation (c.392G>A) leads to the formation of a premature stop codon followed by the formation of a truncated MERTK protein (p.Trp131*) product which also predicted to be disease causing. Hence, these three mutations are loss‐of‐function mutations. These three mutations were absent in unaffected family members and in 100 normal healthy controls.ConclusionOur present study also demonstrates the significance of targeted next generation sequencing in determining the genetic basis of RP.
Young adult patients’ experience of living with mechanical circulatory support: A phenomenological hermeneutical study
Authors: Lachonius Maria , Hederstedt Karl , Axelsson Åsa B. 
Published: February 2019

AbstractAimTo describe young adult patients’ experiences of living with a mechanical circulatory support (MSC) as a bridge to heart transplantation and impact of self‐efficacy.DesignA qualitative and explorative interview study.MethodsEight interviews with adult participants were conducted and analysed using the phenomenological hermeneutical method.ResultsAn overall theme, “Navigating from helplessness to feeling strong in the new reality,” and three themes were identified: “Feeling homeless in a changed reality” describes the experience of suddenly falling ill and the loneliness caused by the disease; “Finding my own inner resources” shows that the interviewees found the strength to fight for their lives and began to regain control of their situation; and “Adapting to my new reality” describes the importance of finding strength from others and being able to see MCS as a friend providing respite from the disease. Self‐efficacy beliefs play a significant role in the process that the participants went through.
Anticipating the effects of restricting high‐dose preparations of strong opioids in Australia: A population‐based analysis to inform the current policy debate
Authors: Zheng Danni , Narayan Sujita W. , Zoega Helga , Litchfield Melisa , Buckley Nicholas A. , Pearson Sallie‐Anne , Schaffer Andrea L. 
Published: February 2019

AbstractPurposeCountries worldwide are developing a variety of strategies to combat the opioid epidemic, such as restricting access to high‐strength opioid formulations. We aimed to examine the dispensing patterns of strong opioids by dose units (DUs), age, and sex.MethodsWe used Australian population‐level dispensing data from January 2003 to December 2015 and categorised strong opioids by DU: very low, low, moderate, and high, corresponding to total daily doses of less than or equal to 25, 26 to 50, 51 to 100, and greater than 100 morphine milligramme equivalents, respectively. We measured trends in strong opioid use as dispensings/1000 population/year and stratified dispensing in 2015 by patient age and sex.ResultsFrom 2003 to 2015, strong opioid dispensing of very low, low, moderate, and high DU increased 6.7‐, 6.2‐, 2.2‐, and 1.8‐fold, respectively. The increase in very low and low DU dispensing was driven primarily by oxycodone (5, 10, and 15 mg tablets and capsules) and buprenorphine transdermal patches. In 2015, the number of dispensings/1000 population for very low, low, moderate, and high DU were 180.3, 77.0, 52.7, and 34.8, respectively. Females aged greater than or equal to 85 years had the highest opioid use, ranging from 157.1 dispensings/1000 population for high DU to 2104.5 dispensings/1000 population for very low DU. In contrast, the high DU dispensings in males aged 25 to 64 years exceeded their female counterparts by approximately 1.3‐fold.ConclusionRelative to moderate and high DU strong opioids, dispensing of very low and low DU strong opioids increased dramatically during the study period in Australia. Future studies investigating opioids use and harms in elderly females and males between 25 to 64 years are warranted.
Proteomic and Post‐Translational Modification Profiling of Exosome‐Mimetic Nanovesicles Compared to Exosomes
Authors: Nasiri Kenari Amirmohammad , Kastaniegaard Kenneth , Greening David W. , Shambrook Mitch , Stensballe Allan , Cheng Lesley , Hill Andrew F. 
Published: February 2019

AbstractIssues associated with upscaling exosome production for therapeutic use may be overcome through utilizing artificial exosomes. Cell‐derived mimetic nanovesicles (M‐NVs) are a potentially promising alternative to exosomes for clinical applicability, demonstrating higher yield without incumbent production and isolation issues. Although several studies have shown that M‐NVs have similar morphology, size and therapeutic potential compared to exosomes, comprehensive characterization and to what extent M‐NVs components mimic exosomes remain elusive. M‐NVs were generated through the extrusion of cells and proteomic profiling demonstrated an enrichment of proteins associated with membrane and cytosolic components. The proteomic data herein reveal a subset of proteins that are highly abundant in M‐NVs in comparison to exosomes. M‐NVs contain proteins that largely represent the parental cell proteome, whereas the profile of exosomal proteins highlight their endosomally derived origin. This advantage of M‐NVs alleviates the necessity of endosomal sorting of endogenous therapeutic proteins or RNA into exosomes. This study also highlights differences in protein post‐translational modifications among M‐NVs, as distinct from exosomes. Overall this study provides key insights into defining the proteome composition of M‐NVs as a distinct from exosomes, and the potential advantage of M‐NVs as an alternative nanocarrier when spontaneous endosomal sorting of therapeutics are limited.
Inspecting fluctuation and coordination around chromophore inside green fluorescent protein from water to nonpolar solvent
Authors: Dai Liqiang , Zhang Bo , Cui Shuxun , Yu Jin 
Published: February 2019

AbstractGreen fluorescent protein (GFP) is a widely used biomarker that demands systematical rational approaches to its structure function redesign. In this work, we mainly utilized atomistic molecular dynamics simulations to inspect and visualize internal fluctuation and coordination around chromophore inside GFP, from water to nonpolar octane solvent. We found that GFP not only maintains its β‐barrel structure well into the octane, but also sustains internal residue and water coordination to position the chromophore stably while suppress dihedral fluctuations of the chromophore, so that functional robustness of GFP is achieved. Our accompanied fluorescence microscope measurements accordingly confirmed the GFP functioning into the octane. Furthermore, we identified that crucial water sites inside GFP along with permeable pores on the β‐barrel of the protein are largely preserved from the water to the octane solvent, which allows sufficiently fast exchanges of internal water with the bulk or with the water layer kept on the surface of the protein. By additionally pulling GFP from bulk water to octane, we suggest that the GFP function can be well maintained into the nonpolar solvent as long as, first, the protein does not denature in the nonpolar solvent nor across the polar‐nonpolar solvent interface; second, a minimal set of water molecules are in accompany with the protein; third, the nonpolar solvent molecules may need to be large enough to be nonpermeable via the water pores on the β‐barrel.
On return period and probability of failure in hydrology
Authors: Volpi Elena 
Published: February 2019

The return period measures the rareness of extreme events such as floods and droughts that might cause huge damages to the society and the environment; hence, it lies at the heart of hydraulic design and risk assessment problems. Indeed, return period is a commonly applied probabilistic concept in the hydrologic literature, which has attracted renewed interest stimulated by the need of efficiently dealing with complex processes in a changing environment. In this study, the concept of return period and the related risk of failure are presented by making use of a general mathematical framework. It helps for a better understanding of the return period and risk of failure formulations that are commonly adopted in practical engineering applications. The framework can be further applied under more general conditions. In particular, the extension of the return period concept to nonstationary and time‐dependent cases is discussed herein, by relaxing the hypotheses commonly (and sometimes implicitly) assumed that allows to derive simple analytic formulations.This article is categorized under:

Engineering Water > Planning Water
Engineering Water > Methods
Science of Water > Methods
Description of Tylodelphys darbyi n. sp. (Trematoda: Diplostomidae) from the threatened Australasian crested grebe (Podiceps cristatus australis, Gould 1844) and linking of its life-cycle stages
Authors: Presswell B. , Blasco-Costa I. 
Published: February 2019

Abstract
Species of the genus Tylodelphys (Diplostomidae) have a cosmopolitan distribution. Metacercariae of these species infect the eye, brain, pericardial sac or body cavity of fish second intermediate hosts, and the adults are found in piscivorous birds of many orders. An unnamed species of Tylodelphys from the eyes of bullies (Gobiomorphus cotidianus) was characterized molecularly and morphologically as a metacercaria in a previous study, in which it was predicted that the adult of this species would be found in the Australasian crested grebe. Two specimens of this bird became available and specimens of the unnamed Tylodelphys species were, indeed, found in them, confirmed by identity of genetic sequence data. Found to differ morphologically from its congeners, the new species is here described as Tylodelphys darbyi n. sp. Three species are closest to the new species in morphology: Tylodelphys glossoides, T. immer and T. podicipina robrauschi. Compared with T. darbyi n. sp. these three species are slightly larger and possess longer eggs. Tylodelphys glossoides also differs in having a wider oral sucker and T. podicipina robrauschi in having comma- or kidney-shaped pseudosuckers and an ovary that reaches a larger size, along with higher upper limits for body width, hind body and sucker width, holdfast and oesophagus length, and pharynx, pseudosucker and testes length and width. Tylodelphys immer also differs from T. darbyi n. sp. in having a shorter ventral sucker and the largest pseudosuckers of any Tylodelphys species.
Intergenerational response of steroidogenesis-related genes to maternal malnutrition
Authors: Harrath Abdel Halim , Alrezaki Abdulkarem , Alwasel Saleh H. , Semlali Abdelhabib 
Published: February 2019

AbstractWe sought to examine whether rat maternal food restriction (MFR) affects the expression of steroidogenesis-related genes Cyp19, Cyp17a1, Insl3 and Gdf-9 in the ovaries of offspring from the first (FRG1) and second (FRG2) generations at pre-pubertal age (week 4) and during adulthood (week 8). At week 4, MFR significantly increased the expression of RNAs for all analyzed genes in both FRG1 and FRG2 females, which may indicate that MFR affects the onset of the reproductive lifespan, by inducing early pubertal onset. At week 8, the Cyp19 gene was still upregulated in MRF-subjected animals (Cyp19: P=0.0049 and P=0.0508 in FRG1 and FRG2, respectively), but MFR induced a significant decrease in Cyp17 and Gdf-9 gene expression in the offspring of both FRG1 and FRG2 females when compared with the controls (Cyp17: P=0.0018 and P=0.0016, respectively; Gdf-9: P=0.0047 and P=0.0023, respectively). This suggests that females at week 8, which should normally be in their optimal reproductive capacity, experience premature ovarian aging. At week 4, the activation of Cyp19 and Cyp17 was higher in the FRG1 ovaries than in the FRG2 ovaries, whereas the extent of Insl3 and Gdf-9 activation was lower in the FRG1 ovaries. This may indicate that FRG2 females were more vulnerable to MFR than their mothers (FRG1) and grandmothers, which is consistent with the ‘predictive adaptive response’ hypothesis. Our findings reveal that MFR may induce intergenerational ovarian changes as an adaptive response to ensure reproductive success before death.