bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal smoking during pregnancy and autism: using causal inference methods in a birth cohort study
Authors: Caramaschi Doretta , Taylor Amy E. , Richmond Rebecca C. , Havdahl Karoline Alexandra , Golding Jean , Relton Caroline L. , Munafò Marcus R. , Davey Smith George , Rai Dheeraj 
Published: November 2018

AbstractAn association between maternal smoking in pregnancy and autism may be biologically plausible, but the evidence to date is inconsistent. We aimed to investigate the causal relationship between maternal smoking during pregnancy and offspring autism using conventional analysis and causal inference methods. In the Avon Longitudinal Study of Parents and Children we investigated the association of maternal smoking during pregnancy (exposure) with offspring autism spectrum disorder (ASD) or possible ASD diagnosis (n = 11,946) and high scores on four autism-related traits (outcomes) (n = 7402–9152). Maternal smoking was self-reported and also measured using an epigenetic score (n = 866–964). Partner’s smoking was used as a negative control for intrauterine exposure (n = 6616–10,995). Mendelian randomisation (n = 1002–2037) was carried out using a genetic variant at the CHRNA3 locus in maternal DNA as a proxy for heaviness of smoking. In observational analysis, we observed an association between smoking during pregnancy and impairments in social communication [OR = 1.56, 95% CI = 1.29, 1.87] and repetitive behaviours, but multivariable adjustment suggested evidence for confounding. There was weaker evidence of such association for the other traits or a diagnosis of autism. The magnitude of association for partner’s smoking with impairments in social communication was similar [OR = 1.56, 95% CI = 1.30, 1.87] suggesting potential for shared confounding. There was weak evidence for an association of the epigenetic score or genetic variation at CHRNA3 with ASD or any of the autism-related traits. In conclusion, using several analytic methods, we did not find enough evidence to support a causal association between maternal smoking during pregnancy and offspring autism or related traits.
Therapeutic faecal microbiota transplantation controls intestinal inflammation through IL10 secretion by immune cells
Authors: Burrello Claudia , Garavaglia Federica , Cribiù Fulvia Milena , Ercoli Giulia , Lopez Gianluca , Troisi Jacopo , Colucci Angelo , Guglietta Silvia , Carloni Sara , Guglielmetti Simone , Taverniti Valentina , Nizzoli Giulia , Bosari Silvano , Caprioli Flavio , Rescigno Maria , Facciotti Federica 
Published: November 2018

AbstractAlteration of the gut microbiota has been associated with different gastrointestinal disorders. Normobiosis restoration by faecal microbiota transplantation (FMT) is considered a promising therapeutic approach, even if the mechanisms underlying its efficacy are at present largely unknown. Here we sought to elucidate the functional effects of therapeutic FMT administration during experimental colitis on innate and adaptive immune responses in the intestinal mucosa. We show that therapeutic FMT reduces colonic inflammation and initiates the restoration of intestinal homeostasis through the simultaneous activation of different immune-mediated pathways, ultimately leading to IL-10 production by innate and adaptive immune cells, including CD4+ T cells, iNKT cells and Antigen Presenting Cells (APC), and reduces the ability of dendritic cells, monocytes and macrophages to present MHCII-dependent bacterial antigens to colonic T cells. These results demonstrate the capability of FMT to therapeutically control intestinal experimental colitis and poses FMT as a valuable therapeutic option in immune-related pathologies.
H2 roaming chemistry and the formation of H3+ from organic molecules in strong laser fields
Authors: Ekanayake Nagitha , Severt Travis , Nairat Muath , Weingartz Nicholas P. , Farris Benjamin M. , Kaderiya Balram , Feizollah Peyman , Jochim Bethany , Ziaee Farzaneh , Borne Kurtis , Raju P. Kanaka , Carnes Kevin D. , Rolles Daniel , Rudenko Artem , Levine Benjamin G. , Jackson James E. , Ben-Itzhak Itzik , Dantus Marcos 
Published: November 2018

AbstractRoaming mechanisms, involving the brief generation of a neutral atom or molecule that stays in the vicinity before reacting with the remaining atoms of the precursor, are providing valuable insights into previously unexplained chemical reactions. Here, the mechanistic details and femtosecond time-resolved dynamics of H3+ formation from a series of alcohols with varying primary carbon chain lengths are obtained through a combination of strong-field laser excitation studies and ab initio molecular dynamics calculations. For small alcohols, four distinct pathways involving hydrogen migration and H2 roaming prior to H3+ formation are uncovered. Despite the increased number of hydrogens and possible combinations leading to H3+ formation, the yield decreases as the carbon chain length increases. The fundamental mechanistic findings presented here explore the formation of H3+, the most important ion in interstellar chemistry, through H2 roaming occurring in ionic species.
ATP-dependent membrane remodeling links EHD1 functions to endocytic recycling
Authors: Deo Raunaq , Kushwah Manish S. , Kamerkar Sukrut C. , Kadam Nagesh Y. , Dar Srishti , Babu Kavita , Srivastava Anand , Pucadyil Thomas J. 
Published: November 2018

AbstractEndocytic and recycling pathways generate cargo-laden transport carriers by membrane fission. Classical dynamins, which generate transport carriers during endocytosis, constrict and cause fission of membrane tubes in response to GTP hydrolysis. Relatively, less is known about the ATP-binding Eps15-homology domain-containing protein1 (EHD1), a dynamin family member that functions at the endocytic-recycling compartment. Here, we show using cross complementation assays in C. elegans that EHD1’s membrane binding and ATP hydrolysis activities are necessary for endocytic recycling. Further, we show that ATP-bound EHD1 forms membrane-active scaffolds that bulge tubular model membranes. ATP hydrolysis promotes scaffold self-assembly, causing the bulge to extend and thin down intermediate regions on the tube. On tubes below 25 nm in radius, such thinning leads to scission. Molecular dynamics simulations corroborate this scission pathway. Deletion of N-terminal residues causes defects in stable scaffolding, scission and endocytic recycling. Thus, ATP hydrolysis-dependent membrane remodeling links EHD1 functions to endocytic recycling.
Layer-edge device of two-dimensional hybrid perovskites
Authors: Cheng Bin , Li Ting-You , Wei Pai-Chun , Yin Jun , Ho Kang-Ting , Retamal José Ramón Durán , Mohammed Omar F. , He Jr-Hau 
Published: November 2018

AbstractTwo dimensional layered organic-inorganic hybrid perovskites (2D perovskites) are potential candidates for next generation photovoltaic device. Especially, the out-of-plane surface perpendicular to the superlattice plane of 2D perovskites (layer-edge surface) has presented several exotic behaviors, such as layer-edge states which are found to be crucial for improving the efficiency of 2D perovskite solar cells. However, fundamental research on transport properties of layer-edge surface is still absent. In this report, we observe the electronic and opto-electronic behavior in layer-edge device of 2D perovskites. The dark and photo currents are demonstrated to strongly depend on the crystallographic orientation in layer-edge device, and such anisotropic properties, together with photo response, are related to the thickness of inorganic layers. Finally, due to the abundant hydroxyl groups, water molecules are easy to condense on the layer-edge surface, and the conductance is extremely sensitive to the humidity environment, indicating a potential application of humidity sensor.
Population coding of valence in the basolateral amygdala
Authors: Zhang Xian , Li Bo 
Published: November 2018

AbstractThe basolateral amygdala (BLA) plays important roles in associative learning, by representing conditioned stimuli (CSs) and unconditioned stimuli (USs), and by forming associations between CSs and USs. However, how such associations are formed and updated remains unclear. Here we show that associative learning driven by reward and punishment profoundly alters BLA population responses, reducing noise correlations and transforming the representations of CSs to resemble the valence-specific representations of USs. This transformation is accompanied by the emergence of prevalent inhibitory CS and US responses, and by the plasticity of CS responses in individual BLA neurons. During reversal learning wherein the expected valences are reversed, BLA population CS representations are remapped onto ensembles representing the opposite valences and predict the switching in valence-specific behaviors. Our results reveal how signals predictive of opposing valences in the BLA evolve during learning, and how these signals are updated during reversal learning thereby guiding flexible behaviors.
Wavepacket insights into the photoprotection mechanism of the UV filter methyl anthranilate
Authors: Rodrigues Natércia d. N. , Cole-Filipiak Neil C. , Blodgett Karl N. , Abeysekera Chamara , Zwier Timothy S. , Stavros Vasilios G. 
Published: November 2018

AbstractMeradimate is a broad-spectrum ultraviolet absorber used as a chemical filter in commercial sunscreens. Herein, we explore the ultrafast photodynamics occurring in methyl anthranilate (precursor to Meradimate) immediately after photoexcitation with ultraviolet radiation to understand the mechanisms underpinning Meradimate photoprotection. Using time-resolved photoelectron spectroscopy, signal from the first singlet excited state of methyl anthranilate shows an oscillatory behavior, i.e., quantum beats. Our studies reveal a dependence of the observed beating frequencies on photoexcitation wavelength and photoelectron kinetic energy, unveiling the different Franck-Condon overlaps between the vibrational levels of the ground electronic, first electronic excited, and ground cationic states of methyl anthranilate. By evaluating the behavior of these beats with increasing photon energy, we find evidence for intramolecular vibrational energy redistribution on the first electronic excited state. Such energy redistribution hinders efficient relaxation of the electronic excited state, making methyl anthranilate a poor choice for an efficient, efficacious sunscreen chemical filter.
Enrichment of the fetal fraction in non-invasive prenatal screening reduces maternal background interference
Authors: Liang Bo , Li Hong , He Quanze , Li Haibo , Kong Lingyin , Xuan Liming , Xia Yingying , Shen Jingjing , Mao Yan , Li Yixue , Wang Ting , Zhao Yi-Lei 
Published: November 2018

AbstractMeasurement of cell-free fetal DNA (cffDNA) is an indispensable process for non-invasive prenatal screening (NIPS). According to recent studies, cffDNA in maternal plasma can be enriched for various lengths of fragments, and a sufficient amount of cffDNA can effectively eliminate background interference on the part of maternal DNA. Therefore, we developed a simple and effective separation method, improved NIPS (iNIPS), that enriches the fetal fraction and improves the accuracy of NIPS for fetal aneuploid detection. We adopted a novel strategy to achieve enrichment of 125–135 bp cell-free DNA (cfDNA) by e-gel electrophoresis. To evaluate clinical performance, we compared NIPS and iNIPS results from 2153 retrospective clinical samples. Of the 22 samples with NIPS results of “no call”, 17 samples were reclassified as “unaffected” (9 cases of chr13, 5 cases of chr18, and 3 cases of chr21); 2 samples remained classified as “no call” (1 case of chr18 and 1 case of chr21); and 3 samples were identified as T21 by iNIPS. The average increase in abundance of cfDNA fragments of 125–135 bp was 2.5 times, and the average decrease in maternal background interference was 1.3 times. On this basis, the detection of fetal aneuploidy was highly improved with the fetal fraction as low as 2%; iNIPS achieved 100% sensitivity and 99.90% specificity in retrospective samples.
Tuning structure and mechanical properties of Ta-C coatings by N-alloying and vacancy population
Authors: Glechner T. , Mayrhofer P. H. , Holec D. , Fritze S. , Lewin E. , Paneta V. , Primetzhofer D. , Kolozsvári S. , Riedl H. 
Published: November 2018

AbstractTailoring mechanical properties of transition metal carbides by substituting carbon with nitrogen atoms is a highly interesting approach, as thereby the bonding state changes towards a more metallic like character and thus ductility can be increased. Based on ab initio calculations we could prove experimentally, that up to a nitrogen content of about 68% on the non-metallic sublattice, Ta-C-N crystals prevail a face centered cubic structure for sputter deposited thin films. The cubic structure is partly stabilized by non-metallic as well as Ta vacancies – the latter are decisive for nitrogen rich compositions. With increasing nitrogen content, the originally super-hard fcc-TaC0.71 thin films soften from 40 GPa to 26 GPa for TaC0.33N0.67, accompanied by a decrease of the indentation modulus. With increasing nitrogen on the non-metallic sublattice (hence, decreasing C) the damage tolerance of Ta-C based coatings increases, when characterized after the Pugh and Pettifor criteria. Consequently, varying the non-metallic sublattice population allows for an effective tuning and designing of intrinsic coating properties.
The Effect of Memory in Inducing Pleasant Emotions with Musical and Pictorial Stimuli
Authors: Maksimainen Johanna , Wikgren Jan , Eerola Tuomas , Saarikallio Suvi 
Published: November 2018

AbstractMusic is known to evoke emotions through a range of mechanisms, but empirical investigation into the mechanisms underlying different emotions is sparse. This study investigated how affective experiences to music and pictures vary when induced by personal memories or mere stimulus features. Prior to the experiment, participants were asked to select eight types of stimuli according to distinct criteria concerning the emotion induction mechanism and valence. In the experiment, participants (N = 30) evaluated their affective experiences with the self-chosen material. EEG was recorded throughout the session. The results showed certain interaction effects of mechanism (memory vs. stimulus features), emotional valence of the stimulus (pleasant vs. unpleasant), and stimulus modality (music vs. pictures). While effects were mainly similar in music and pictures, the findings suggest that when personal memories are involved, stronger positive emotions were experienced in the context of music, even when the music was experienced as unpleasant. Memory generally enhanced social emotions specifically in pleasant conditions. As for sadness and melancholia, stimulus features did not evoke negative experiences; however, these emotions increased strongly with the involvement of memory, particularly in the condition of unpleasant music. Analysis of EEG-data corroborated the findings by relating frontomedial theta activity to memory-evoking material.
iTRAQ-based quantitative tissue proteomic analysis of differentially expressed proteins (DEPs) in non-transgenic and transgenic soybean seeds
Authors: Liu Weixiao , Xu Wentao , Li Liang , Dong Mei , Wan Yusong , He Xiaoyun , Huang Kunlun , Jin Wujun 
Published: November 2018

AbstractThe unintended effects of transgenesis have increased food safety concerns, meriting comprehensive evaluation. Proteomic profiling provides an approach to directly assess the unintended effects. Herein, the isobaric tags for relative and absolute quantitation (iTRAQ) comparative proteomic approach was employed to evaluate proteomic profile differences in seed cotyledons from 4 genetically modified (GM) and 3 natural genotypic soybean lines. Compared with their non-GM parents, there were 67, 61, 13 and 22 differentially expressed proteins (DEPs) in MON87705, MON87701 × MON89788, MON87708, and FG72. Overall, 170 DEPs were identified in the 3 GM soybean lines with the same parents, but 232 DEPs were identified in the 3 natural soybean lines. Thus, the differences in protein expression among the genotypic varieties were greater than those caused by GM. When considering ≥2 replicates, 4 common DEPs (cDEPs) were identified in the 3 different GM soybean lines with the same parents and 6 cDEPs were identified in the 3 natural varieties. However, when considering 3 replicates, no cDEPs were identified. Regardless of whether ≥2 or 3 replicates were considered, no cDEPs were identified among the 4 GM soybean lines. Therefore, no feedback due to GM was observed at the common protein level in this study.
Lecithin-Stabilized Polymeric Micelles (LsbPMs) for Delivering Quercetin: Pharmacokinetic Studies and Therapeutic Effects of Quercetin Alone and in Combination with Doxorubicin
Authors: Chang Chia-En , Hsieh Chien-Ming , Huang Sheng-Chin , Su Chia-Yu , Sheu Ming-Thau , Ho Hsiu-O. 
Published: November 2018

AbstractIn this study, lecithin-stabilized polymeric micelles (LsbPMs) were prepared to load quercetin (QUE) in order to improve its bioavailability and increase its antitumor activity. Its combination with doxorubicin (DOX) to minimize DOX-mediated cardiac toxicity and increase the antitumor activity of QUE-loaded LsbPMs was also examined. LsbPMs were prepared following a previously reported procedure. Results demonstrated that optimal QUE-loaded LsbPMs contained quercetin, D-α-tocopheryl polyethylene glycol succinate, and lecithin at a weight ratio of 6:40:80. Drug-release studies showed that QUE released from LsbPMs followed a controlled release pattern. A cytotoxicity assay revealed that QUE-loaded LsbPMs had significant anticancer activities against MCF-7, SKBR-3, and MDA-MB-231 human breast cancer cells and CT26 mouse colon cancer cells. In animal studies, intravenous administration of QUE-loaded LsbPMs resulted in efficient growth inhibition of CT26 colon cancer cells in a Balb/c mice model. In a pharmacokinetics study compared to free QUE, intravenous and oral administration of QUE-loaded LsbPMs was found to have significantly increased the relative bioavailability to 158% and 360%, respectively, and the absolute bioavailability to 5.13%. The effect of QUE-loaded LsbPMs in combination with DOX resulted in efficient growth inhibition of CT26 colon cancer cells and reduced cardiac toxicity in the Balb/c mice model.
Akinetic swept-source optical coherence tomography based on a pulse-modulated active mode locking fiber laser for human retinal imaging
Authors: Lee Hwi Don , Kim Gyeong Hun , Shin Jun Geun , Lee Boram , Kim Chang-Seok , Eom Tae Joong 
Published: November 2018

AbstractOptical coherence tomography (OCT) is a noninvasive imaging modality that can provide high-resolution, cross-sectional images of tissues. Especially in retinal imaging, OCT has become one of the most valuable imaging tools for diagnosing eye diseases. Considering the scattering and absorption properties of the eye, the 1000-nm OCT system is preferred for retinal imaging. In this study, we describe the use of an akinetic swept-source OCT system based on a pulse-modulated active mode locking (AML) fiber laser at a 1080-nm wavelength for in-vivo human retinal imaging. The akinetic AML wavelength-swept fiber laser was constructed with polarization-maintaining fiber that has an average linewidth of 0.625 nm, a spectral bandwidth of 81.15 nm, and duty ratio of 90% without the buffering method. We successfully obtained in-vivo human retinal images using the proposed OCT system without the additional k-clock and the frequency shifter that provides a wide field of view of 43.1°. The main retina layers, such as the retinal pigment epithelium, can be distinguished from the OCT image with an axial resolution of 6.3 μm with this OCT system.
BK channel blocker paxilline attenuates thalidomide-caused synaptic and cognitive dysfunctions in mice
Authors: Choi Tae-Yong , Lee Seung-Hyun , Kim Soo-Jeong , Jo Youhwa , Park Chul-Seung , Choi Se-Young 
Published: November 2018

AbstractThalidomide is a widely prescribed immunomodulatory drug (iMiD) for multiple myeloma, but causes reversible memory loss in humans. However, how thalidomide causes cognitive dysfunction at a cellular and molecular level has not been demonstrated. We studied the effect of thalidomide on synaptic functions and cognitive behaviors using a mouse model. Thalidomide led to cognitive deficits in learning behavior in a passive avoidance test and in a novel object recognition test, increased anxiety in an elevated plus maze test, and increased depressive behaviors in a tail suspension test. Interestingly, thalidomide elevated big- or large-conductance, calcium-activated K+ (BK) channel expression in the plasma membrane and BK channel activity in the hippocampus. Thalidomide also increased the paired pulse ratio of excitatory postsynaptic current (EPSC), which suggests a decreased probability of glutamate release. Furthermore, the changes in the paired pulse ratio and in BK channel activity were blocked by paxilline, a BK channel blocker. Finally, we found that thalidomide-induced cognitive dysfunctions were restored by paxilline treatment. These results suggest that thalidomide-mediated BK channel hyperfunction is responsible for the pathological mechanism of thalidomide-associated reversible memory loss.
Asteroseismology of evolved stars to constrain the internal transport of angular momentum
Authors: Eggenberger P. , Deheuvels S. , Miglio A. , Ekström S. , Georgy C. , Meynet G. , Lagarde N. , Salmon S. , Buldgen G. , Montalbán J. , Spada F. , Ballot J. 
Published: November 2018

Context. The observations of solar-like oscillations in evolved stars have brought important constraints on their internal rotation rates. To correctly reproduce these data, an efficient transport mechanism is needed in addition to the transport of angular momentum by meridional circulation and shear instability. The efficiency of this undetermined process is found to increase both with the mass and the evolutionary stage during the red giant phase.
Aims. We study the efficiency of the transport of angular momentum during the subgiant phase.
Methods. The efficiency of the unknown transport mechanism is determined during the subgiant phase by comparing rotating models computed with an additional corresponding viscosity to the asteroseismic measurements of both core and surface-rotation rates for six subgiants observed by the Kepler spacecraft. We then investigate the change in the efficiency of this transport of angular momentum with stellar mass and evolution during the subgiant phase.
Results. The precise asteroseismic measurements of both core and surface-rotation rates available for the six Kepler targets enable a precise determination of the efficiency of the transport of angular momentum needed for each of these subgiants. These results are found to be insensitive to all the uncertainties related to the modelling of rotational effects before the post-main sequence (poMS) phase. An interesting exception in this context is the case of young subgiants (typical values of log(g) close to 4), because their rotational properties are sensitive to the degree of radial differential rotation on the main sequence (MS). These young subgiants constitute therefore perfect targets to constrain the transport of angular momentum on the MS from asteroseismic observations of evolved stars. As for red giants, we find that the efficiency of the additional transport process increases with the mass of the star during the subgiant phase. However, the efficiency of this undetermined mechanism decreases with evolution during the subgiant phase, contrary to what is found for red giants. Consequently, a transport process with an efficiency that increases with the degree of radial differential rotation cannot account for the core-rotation rates of subgiants, while it correctly reproduces the rotation rates of red giant stars. This suggests that the physical nature of the additional mechanism needed for the internal transport of angular momentum may be different in subgiant and red giant stars.