bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D-Printed Pneumatic Microfluidic Mixer for Colorimetric Detection of Listeria monocytogenes
Authors: Yao Ping , Wang Ronghui , Xi Xinge , Li Yanbin , Tung Steve 
Published: June 2019

Abstract. 3D printing can significantly improve the current fabrication techniques for microfluidic devices due to its ability to create truly 3D structures in a single step. In this study, an active pneumatic microfluidic mixer was designed and fabricated using an extrusion-based 3D printer and used for rapid detection of . The printed material of the mixer is flexible, semi-transparent, and inexpensive. The fabrication time is significantly shorter than the traditional micromolding process. The printed mixer consists of two pneumatic air chambers and one mixing chamber designed for a fluidic sample size of 100 µL. The length, width, and height of the mixer chip are 13, 12.7, and 9 mm, respectively. The performance of the mixer was tested for different actuation frequencies and pneumatic pressures. The completed 3D-printed mixer was successfully applied to the colorimetric detection of for a concentration range from 102 to 108 cfu mL-1 using an enzyme-linked immunosorbent assay. The experimental results showed that the microfluidic mixer could enhance the mixing efficiency of the fluidic sample through pneumatically actuated diaphragms. In addition, the mixer could accelerate the color development caused by target , and the observed color changes could be discriminated within 5 min by naked eye. The present work will contribute to the development and optimization of a prototype for rapid detection of in food samples. It provides an effective technical approach to realize the fabrication of low-cost microfluidic chips for efficient reagent mixing in microscale biochemical detection systems. Keywords: 3D printing, Listeria monocytogenes, Microfluidic mixer, Rapid detection.
Oscillating Magnetic Field (OMF) Based Supercooling Preservation of Fresh-Cut Honeydew Melon
Authors: Her Jae-Young , Kang Taiyoung , Hoptowit Raymond , Jun Soojin 
Published: June 2019

Abstract. An oscillating magnetic field (OMF) was used to extend the supercooled state in honeydew melon samples. The internal temperature of the samples under OMF treatment was kept at -5.5°C during a 21-day storage period without freezing. To inhibit ice crystal nucleation, the OMF treatment was controlled using a repeated sequence of on/off cycles with durations of 120/420 s. Quality factors of the supercooled samples were measured and compared with fresh, refrigerated, and frozen samples. Factors such as microbiological analysis, drip loss, pH, titratable acidity, and soluble solids contents indicated that the overall quality of the supercooled samples after 21 days was not significantly different (p &gt; 0.05) from that of fresh samples. Although a few cells in the supercooled samples had undulated walls compared to fresh samples under microscopic analysis, the cells were closely bonded to each other, and distorted cells were not found. Keywords: Fresh-cut fruits, Honeydew melon, Oscillating magnetic fields, Quality assessment, Subzero preservation.
Retrospective analysis of 73 cases of elastofibroma
Authors: Haihua R , Xiaobing W , Jie P , Xinxin H 
Published: June 2019

Objective Elastofibroma is a rare soft-tissue tumour. This study retrospectively analysed and summarised the clinical, imaging and typical pathological features, together with the short- and long-term surgical outcomes of patients with pathologically confirmed soft-tissue elastofibroma to improve their management. Materials and methods We enrolled 73 patients with pathologically confirmed soft-tissue elastofibroma from January 2010 to December 2018. The general, clinical, diagnostic and treatment-related data, operation notes, pathological examination results and follow-up status were obtained by reviewing inpatient medical records. Disease onset age, sex, tumour location and size were statistically analysed using the chi square and rank sum tests. Results A total of 90 lesions from 73 patients were examined. Among these, 56 patients had single lesions: 27 were under the right scapula, 26 were under the left scapula, 1 at the umbilicus, 1 on the aortic valve, 1 on the right hip and 17 at the bilateral inferior angles of the scapula. The average age at onset was 56.4 years (range: 6–82 years). The male-to-female incidence ratio was about one to three. Tumour diameter and follow-up duration ranged from 2cm to 12cm and from one month to nine years, respectively; recurrence was not observed. The main postoperative complication was wound effusion, occurring in 24 sites among the 90 lesions, corresponding to an incidence rate of 26.7%. Conclusions A correct diagnosis of elastofibroma can be made prior to surgical resection by examining typical clinical features and characteristic imaging findings. Short- and long-term outcomes of local excision are good, with no further recurrence.
Factors affecting patient flow in a neurosurgery department
Authors: Irvine S , Awan M , Chharawala F , Bhagawati D , Lawrance N , Peck G , Peterson D , Banerjee S , Camp S 
Published: June 2019

Introduction Patient flow is the process by which movement of patients and clinical productivity is achieved. The objectives of this study were to implement and evaluate the NHS Improvement SAFER patient flow bundle, evaluate the impact of the Red2Green initiative, and assess the impact of frailty on patient flow. Materials and methods All patients admitted to a neurosurgery unit from 1 September to 30 November 2017 were included. Using guidance set out by NHS, data were prospectively collected from daily ward lists and patient notes, including demographics, admission and discharge details, length of stay, anticipated discharge date, red days with reasons and frailty (Rockwood Clinical Frailty Scale). NHS reference costs were used for cost analyses. Results A total of 420 patients (55% elective) were included, totalling 3909 bed days. All patients received daily senior reviews before midday, and anticipated discharge dates were set at daily multidisciplinary team meetings. Ten per cent of patients were discharged before midday. There were 21% (837) red days, significantly more (76%) for emergency patients (639 vs 198 elective; P < 0.001); 63% red days were attributed to awaiting a bed in a local hospital; 25% (106) patients were classed as frail (50 elective), which was associated with a significantly longer length of stay (17.3 vs 6; P < 0.01), and more red days (615 vs 222; p<0.01). Considering excess bed charges and lost revenue (with penalties), red days cost over £1 million per year. Conclusions SAFER has identified areas for improvement in patient flow, with obvious cost implications. It has created a platform for discussion within the referral network and identified a role for a geriatric liaison service.
A multicentre, prospective, observational cohort study of variation in practice in perioperative analgesia strategies in elective laparoscopic colorectal surgery (the LapCoGesic study)
Authors: Brown L , Gray M , Griffiths B , Jones M , Madhavan A , Naru K , Shaban F , Somnath S , Harji D ,   on behalf of NoSTRA (Northern Surgical Trainees Reseach Association)
Published: June 2019

Introduction Enhanced recovery programmes are established as an essential part of laparoscopic colorectal surgery. Optimal pain management is central to the success of an enhanced recovery programme and is acknowledged to be an important patient reported outcome measure. A variety of analgesia strategies are employed in elective laparoscopic colorectal surgery ranging from patient-controlled analgesia to local anaesthetic wound infiltration catheters. However, there is little evidence regarding the optimal analgesia strategy in this cohort of patients. The LapCoGesic study aimed to explore differences in analgesia strategies employed for patients undergoing elective laparoscopic colorectal surgery and to assess whether this variation in practice has an impact on patient-reported and clinical outcomes. Materials and methods A prospective, multicentre, observational cohort study of consecutive patients undergoing elective laparoscopic colorectal resection was undertaken over a two-month period. The primary outcome measure was postoperative pain scores at 24 hours. Data analysis was conducted using SPSS version 22. Results A total of 103 patients undergoing elective laparoscopic colorectal surgery were included in the study. Thoracic epidural was used in 4 (3.9%) patients, spinal diamorphine in 56 (54.4%) patients and patient-controlled analgesia in 77 (74.8%) patients. The use of thoracic epidural and spinal diamorphine were associated with lower pain scores on day 1 postoperatively (P < 0.05). The use of patient-controlled analgesia was associated with significantly higher postoperative pain scores and pain severity. Discussion Postoperative pain is managed in a variable manner in patients undergoing elective colorectal surgery, which has an impact on patient reported outcomes of pain scores and pain severity.
Migraine and other headaches in transient ischemic attacks
Authors: Lebedeva E. R. , Gurary N. M. , Olesen J. 
Published: June 2019

Headache is a common symptom in acute cerebrovascular diseases; however, no studies have evaluated the prevalence of specific headache types in patients with transient ischemic attacks (ТIАs).Objective: to analyze all headaches within the last year and the last week before and during ТIАs.Patients and methods. TIA patients included in the study (female 55% (n=120); mean age, 56.1 years) according to the existing definition of TIAs had a transient neurological dysfunction episode caused by focal brain damage or retinal ischemia for up to 24 hours without forming a new acute heart attack on diffusion-weighted MRI (n=112) or CT (n=8). All the patients were examined by one neurologist within one day after their admission. Patients (female 64% (n=192); mean age, 58.7 years) who had been admitted with a diagnosis of lumbago, lumbar spine osteochondrosis, or gastrointestinal ulcer were examined as a control group. A clinical semistructured face-to-face interview with the patients of both groups was carried out to analyze headache.Results and discussion. The prevalence of migraine without aura during one year before TIA was substantially higher in patients with TIA than in control ones: 20.8 and 7.8%, respectively (p=0.002). Twenty-two (18.3%) patients had sentinel or warning headache within the last week before a TIA that manifested as an increase in and greater frequency of previous headache, as lack of effect of painkillers, and as the emergence of a new type of headaches, which were previously absent. During TIAs, 16 (13.3%) patients developed a new type of headache. Twelve of these 16 patients had migraine-like headache; three patients had headache resembling tension headache; one patient had a thunderclap headache. None of the control patients was found to have a new type of headache. TIAs were significantly more common in the vertebrobasilar basin than in the carotid artery one in patients with headache during the last week before and during TIA.Conclusion. The one year prevalence of migraine was significantly higher in ТIА patients than in control patients, and so was the prevalence of headache within the last week before and during TIA. Migraine-like headache prevailed among the new types of headaches in the development of TIA. A previous headache with a change in characteristics and a new type of headache can be predictors for TIA.
The Basolateral Nucleus of the Amygdala Executes the Parallel Processes of Avoidance and Palatability in the Retrieval of Conditioned Taste Aversion in Male Rats
Authors: Inui Tadashi , Sugishita Tomoaki , Inui-Yamamoto Chizuko , Yasoshima Yasunobu , Shimura Tsuyoshi 
Published: June 2019

AbstractConditioned taste aversion (CTA) is an essential behavior for animal survival. Conditioned animals show avoidance and decreased palatability to a conditioned stimulus (CS) on CTA retrieval. In this study, we aimed to determine whether the basolateral nucleus of the amygdala (BLA) is involved in CTA retrieval and whether avoidance and palatability in CTA retrieval are processed in the BLA. We developed an experimental chamber for time-course analysis of the behavior to approach a spout and lick a CS. In this experimental chamber, we analyzed the behavior of male rats following microinjections of GABAAreceptor agonist muscimol or saline into the BLA. The rats showed two types of approach behavior: they either (1) approached and licked the spout or (2) approached but did not lick the spout. Muscimol injection into the BLA decreased the frequency of the latter type of approach behavior, indicating that BLA inactivation reduced avoidance to the CS. The muscimol injection into the BLA also significantly increased the consumption of the CS. Lick microstructure analysis demonstrated that intra-BLA muscimol significantly increased licking burst number and size, indicating that BLA inactivation attenuated aversion to the CS as large burst licking is an indicator of high palatability. These results suggest that the increase in CS consumption with intra-BLA muscimol injection was due to alterations in approach and aversive responses to the CS. Therefore, we conclude that the BLA plays an essential role in CTA retrieval by parallel processing of avoidance and palatability.
Adolescent Connectedness and Adult Health Outcomes
Authors: Steiner Riley J. , Sheremenko Ganna , Lesesne Catherine , Dittus Patricia J. , Sieving Renee E. , Ethier Kathleen A. 
Published: June 2019

BACKGROUND:
Because little is known about long-term effects of adolescent protective factors across multiple health domains, we examined associations between adolescent connectedness and multiple health-related outcomes in adulthood.

METHODS:
We used weighted data from Waves I and IV of the National Longitudinal Study of Adolescent to Adult Health (n = 14 800). Linear and logistic models were used to examine associations between family and school connectedness in adolescence and self-reported health risk behaviors and experiences in adulthood, including emotional distress, suicidal thoughts and attempts, physical violence victimization and perpetration, intimate partner physical and sexual violence victimization, multiple sex partners, condom use, sexually transmitted infection (STI) diagnosis, prescription drug misuse, and other illicit drug use.

RESULTS:
In multivariable analyses, school connectedness in adolescence had independent protective associations in adulthood, reducing emotional distress and odds of suicidal ideation, physical violence victimization and perpetration, multiple sex partners, STI diagnosis, prescription drug misuse, and other illicit drug use. Similarly, family connectedness had protective effects for emotional distress, all violence indicators, including intimate partner violence, multiple sex partners, STI diagnosis, and both substance use indicators. Compared to individuals with low scores for each type of connectedness, having high levels of both school and family connectedness was associated with 48% to 66% lower odds of health risk behaviors and experiences in adulthood, depending on the outcome.

CONCLUSIONS:
Family and school connectedness may have long-lasting protective effects across multiple health outcomes related to mental health, violence, sexual behavior, and substance use. Increasing both family and school connectedness during adolescence has the potential to promote overall health in adulthood.
PENGUASAAN GURU MATEMATIKA SEKOLAH MENENGAH TERHADAP PEMBELAJARAN INOVATIF BERBASIS PENELITIAN MELALUI KEGIATAN LESSON STUDY
Authors: Rohaeti Euis Eti , Hendriana Heris 
Published: June 2019

This is a descriptive study aimed to examine mathematics teachers’ ability to master various research-based innovative teaching approaches and their ability to plan, implementi, and assess their teaching and learning procesess. The subjects of this study are 16 mathematics teachers from four different districts in West Java who collaborated with 8 post-gradute students of STKIP Siliwangi Bandung as a part of Lesson Study program. The instruments of this study are an observation sheet and writen and oral interview with the teachers. The study revealed that:1) The teachers’ ability on mastering various innovative teaching learning approaches based on a research was at low level; 2) Teachers’ ability on planning, implementing, and assessing their teaching and learning process was at medium level; 3) There is high association between teachers’ ability on mastering various innovative teaching learning approaches based on a research and their abilities on planning and implementing teaching-learning process; 4) There is medium associationbetween teachers’ ability on mastering various innovative teaching learning approaches based on a research and their ability on assessing their teaching-learning process; 5) Lesson Study Activities between teachers and students as an effort to share reseacrh outcomes of university lecturers with school teachers. ABSTRAKPenelitian ini merupakan penelitian deskriptif yang bertujuan menelaah penguasaan guru matematika terhadap beragam pembelajaran inovatif berbasis penelitian yang dikaitkan dengan kemampuan guru dalam merencanakan, melaksanakan dan mengevaluasi pembelajaran yang dilakukannya. Subjek penelitian terdiri dari 16 orang guru sekolah menengah yang berasal dari 4 kabupaten yang berbeda di Jawa Barat yang berkolaborasi dengan 8 mahasiswa pasca sarjana Pendidikan Matematika dari sebuah STKIP di Badung dalam rangka kegiatan Lesson Study. Pengambilan data dilakukan melalui observasi pembelajaran dan wawancara lisan dan tertulis dengan guru tersebut. Hasil penelitian menunjukkan bahwa: 1) Penguasaan guru matematika terhadap beragam pembelajaran inovatif berbasis penelitian masih berada pada level rendah; 2) Kemampuan merencanakan, melaksanakan, dan mengevaluasi pembelajaran berada pada level sedang; 3) Terdapat asosiasi yang tinggi antara penguasaan guru matematika terhadap beragam pembelajaran inovatif berbasis penelitian dengan kemampuan merencanakan dan melaksanakan pembelajaran; 4) Terdapat asosiasi yang sedang antara penguasaan guru matematika terhadap beberapa pembelajaran inovatif berbasis penelitian dengankemampuan mengevaluasi pembelajaran; 5) KegiatanLesson Study antara guru dan mahasiswa merupakan upaya mendiseminasikan hasil penelitian para akademisi kepada guru di sekolah.
Short- and long-term results of angioplastic lobectomy in lung cancer surgery
Authors: Kirshin A. A. , Napolskikh V. M. , Styazhkina S. N. 
Published: June 2019

Aim. To study the oncological expediency of using vascular techniques in organ-saving treatment of lung cancer.
Materials and methods. Over the period of 2009–2016, there were performed 92 lobectomies with resection and reconstruction of the pulmonary artery in patients with non-small carcinoma of the lung.
Results. No intraoperative complications and lethal outcomes were fixed. The postoperative complications were registered in 23 cases (25.3 %). Lethality was 3.3 %. The overall one-year survival rate in patients with stage I–III lung cancer after organ-saving surgery was 96.7 %, three-year – 65.1 %, five-year – 36.6 %, median survival rate – 53.1 months. The recurrence-free survival rate in patients with stage I–III lung cancer was: one-year – 89.9 %, three-year – 65.3 %, five-year – 48.8 %. The median recurrence-free survival rate was 49.8 months.
Conclusions.Angioplastic lobectomy provides an acceptable short and long-term result, which does not yield to the analogous indices for pulmonectomy. It confirms our opinion on the expediency of implementing this procedure regarding non-small carcinoma of the lung.
Application of combined technique for laparotomy wound closure in urgent surgery
Authors: Inyutin A. S. , Fedoseev A. V. , Krymov O. V. , Muravyov S. Yu. , Rustamov V. I. ogly 
Published: June 2019

Aim. To improve the results of patients’ treatment after midline laparotomy by reducing the number of the postoperative ventral hernias and eventrations.
Materials and methods.There were examined 111 urgent patients, operated through the median laparotomic approach. The main group included 48 persons, for whom,on completion of intervention,the white line was sutured with a chess-staggered suture(patent of RF № 2644846, 14.02.18). In the control group, for median aponeurosis interrupted sutures were used.
Results. In the early postoperative period, in the control group there were 2 (3 %) eventrations, in the main group – no eventrations. After one year of observations, in the main group – 3 (6 %) patients with aponeurosis defects by the postoperative scar USI and 2 (4 %) patients with hernias were found out. In the control, one year after the surgery, there were 6 (9.5 %) aponeurosis defects and 5 (8 %) postoperative hernias.
Conclusions. Application of interrupted chess suture in combination with staggered sutures is the way to prevent eventrations and postoperative ventral hernias in urgent surgery.
Morphological characteristic of inguinal lymph nodes in HIV infection
Authors: Gulyaeva N. I. , Freind G. G. , Shmagel N. G. , Korolevskaya L. B. , Saidakova E. V. , Shmagel K. V. 
Published: June 2019

Aim. To assess the morphological changes in inguinal lymph nodes among patients with stage 4a HIV infection, who receive antiretroviral therapy.
Materials and methods. There were examined 12 HIV-infected patients with stage 4a, treated at “Perm Regional Center for Prevention and Fight against AIDS and Infectious Diseases”, who received antiretroviral therapy for 2 years. Two groups of patients were formed: group I – 6 persons, whose CD+ blood lymphocyte number was more than 350 in 1 mcl; group II – 6 persons with CD+ blood lymphocyte number less than 350 in 1 mcl. Inguinal lymph nodes (ILN) were taken under local anesthesia, histological preparations were prepared by traditional scheme, stained with hematoxylin and eosin using Masson three-colour staining. In immunohistochemical reactions expression of Ki-67 and CD+ markers was estimated.
Results.Histoarchitectonics of lymph nodes was changed as a result of massive development of sclerosis in the region of hilum, capsule and trabecules. Against the background of sclerosis, there occurred lymphocyte depletion, change in structure of stromal cells and neoangiogenesis in all the zones of the lymph node. In the regions of sclerosis, death of lymphocytes was revealed. In patients of the second group, more active development of follicles with the centers of reproduction in the lymph node cortical substance, as well as growth of the number of Ki-67 maker-expressing cells was established
Conclusions.In the inguinal lymph nodes, the development of sclerotic processes causes the death of T-lymphocytes, which, in their turn, are the source of lymphotoxin-β formation. Loss of CD+-T-lymphocytes is accompanied by deficit of lymphotoxin-β and induces the loss of fibroblastic reticular cells themselves, which through the production of IL-7 support the vital activity of T-cells.