bluuebunny/crossref_metadata_2025_split huggingface dataset:
Addressing Flow Assurance and Annulus Pressure Management Challenges in HPHT Well
Authors: Mazurenko N. , Riyanto L. 
Published: November 2024

Abstract
HPHT well required open-up after being drilled, completed, and unloaded without impacting topside facilities and existing wells. Flow assurance (i.e., hydrates formation prediction), potential wellhead growth, and annulus management during well open-up required a detailed study. The paper describes a comprehensive approach in HPHT gas well management, involving flow assurance evaluation, backpressure modelling, annulus management plan preparation, and implementation of unified workflow to minimize associated risks and unlock technical potential of HPHT well.
A comprehensive plan was established to address potential backpressure evaluation from HPHT well to existing wells producing from reservoirs with lower formation pressures; flow assurance prediction via hydrates formation modelling; annulus pressure management via nitrogen cushion injection and installation of pressure bleeding lines for respective annuluses; topside management via wellhead growth monitoring during open-up.
Numeric Simulation was carried out to predict potential hydrates formation due to Joule–Thomson effect across the choke and run sensitivity cases for concentrations of hydrate inhibitors and injection rates. Wellhead growth evaluation was carried out via modelling to address risks associated with topside facilities.
Based on hydrates modelling prediction, a 4 bbl of methanol solution was selected for injection at 0.0277 m3/h rate via wellhead to minimize risks associated with hydrates formation due to Joule–Thomson effect across the choke during the early stage of well open-up until pressure drop across the choke reduces and flowline temperature raises. Nitrogen was injected in the Annulus A (at 200 psig) as a cushion and monitored throughout the well bean-up. Maximum allowable and maximum operating pressures were established for Annulus A, B, and C with bleeding lines connected to reduce any potential pressure increase due to thermal expansion. Based on the thermal growth study, the safe limits of minimum 3" and maximum 10" of wellhead growth, were monitored and adhered to safeguard topside facilities and operations. All findings and results of multidisciplinary studies were summarized in a comprehensive well open-up procedure with critical precautions put in place.
As a result, HPHT well open-up successfully commenced in a safe manner, unlocking the well technical potential of 75 MMscfd of gas and 1900 blpd of condensate rate. The success of this pilot is crucial for future HPHT gas well developments with flow assurance and annulus pressure management challenges.
Current approach summarizes the best practices and lessons learned from the successful implementation of hydrates management via methanol injection, backpressure evaluation, and wellhead growth prediction via numerical modelling, and annulus pressure build-up management via nitrogen cushion injection during HPHT gas well start-up that can be applied to future HPHT developments.
Study on the Flow Characteristics of Non-Newtonian Drilling Fluid Through Vibrating Screen Meshes
Authors: Zhang Yang , Han Yongliang , Wu Baokang , Sun Tengfei 
Published: November 2024

Summary
Composite screens are a critical component of drilling fluid shale shakers, and their performance directly affects the recovery efficiency of drilling fluid. This study focuses on the composite screens of shale shakers and simulates the flow of non-Newtonian drilling fluids through different structures of composite screens to investigate the impact of drilling fluid rheological parameters and screen compositions on screening performance. The research results indicate that the flow velocity of the drilling fluid is the highest while passing through the lower screen, and the turbulent kinetic energy (TKE) is at its maximum in the lower screen area, where energy dissipation is also the greatest. An increase in the rheological parameters of the four-parameter model drilling fluid generally increases the difficulty of passing through the screen, with the flow behavior index and viscosity coefficient having a particularly significant impact on the pressure drop coefficient. Compared with the plain composite screen, the herringbone composite screen and plain patterned composite screen have lower pressure drop coefficients, with the herringbone composite screen demonstrating the best screening performance. These findings provide theoretical references for flow analysis and structural optimization of shale shaker screens in drilling fluid applications.
The zinc finger protein560(ZNF560) functions as a novel oncogenic gene in osteosarcoma
Authors: Dong Xiong , Xu Guanhua , Hong Hongxiang , Zhang Jinlong , Cui Zhiming , Yu ZiLiang 
Published: November 2024

Abstract

Background
Abnormal expression of Zinc finger (ZNF) genes is commonly observed in osteosarcoma (OS), the most prevalent malignant bone tumor in children and teenagers. This project focused on the role of ZNF560 in the progress of OS.
Methods
The published datasets including TCGA-SARC and GSE99671 was utilized to screen out the abnormal expression of ZNF560 and associated gene patterns in sarcoma and OS tissues. Prognosis value of ZNF560 was identified in TCGA-SARC and OS cohorts. In order to manipulate ZNF560 expression in HOS and MG63 osteosarcoma (OS) cells, genetic strategies such as shRNA constructs were utilized. The expression patterns of ZNF560 were analyzed through techniques such as immunohistochemistry, Western blotting, and qRT-PCR.
Results
By analyzing data from both the GEO and the Cancer Genome Atlas (TCGA) databases, increased expression of ZNF560 in OS tissues was verified, which was significantly associated with poorer outcomes in osteosarcoma patients both in TCGA-SARC and our own OS cohorts. Additionally, downregulation of ZNF560 resulted in decreased cell viability, fewer colonies, and induced apoptosis of osteosarcoma cells. Moreover, ZNF560 was found to be essential for migration of human osteosarcoma HOS and MG63 cells.
Conclusion
Collectively, these findings suggest that ZNF560 has the potential to serve as a predictive biomarker for osteosarcoma.
First Report of Yambean Mosaic Virus Infection in Butterfly Pea (Clitoria ternatea) in Thailand
Authors: Jenjai Khomkrit , Samarakoon Milan C. 
Published: November 2024

Abstract

Butterfly pea (Clitoria ternatea) plants displaying mottling, vein banding, blistering, and wrinkling symptoms were observed in Chiang Mai province, Thailand, indicating potential potyvirus infection. Three symptomatic leaf samples, immature and mature seed samples, were collected for RNA extraction and tested via RT-PCR using degenerate primers targeting the coat protein (CP) gene of potyviruses. Four samples, excluding mature seeds, tested positive for potyvirus. Mechanical inoculation reproduced symptoms in butterfly pea, senna (Senna occidentalis), and yardlong bean (Vigna unguiculata subsp. sesquipedalis). Phylogenetic analysis confirmed infection by yambean mosaic virus (YBMV), marking the first report of YBMV in butterfly peas and in Thailand.
Higher spatial resolution is not always better: evaluating satellite-sensed sea surface temperature products for a west Pacific coral reef system
Authors: Lachs Liam , Donner Simon , Edwards Alasdair J. , Golbuu Yimnang , Guest James 
Published: November 2024

Abstract

As marine heatwaves and mass coral bleaching events rise in frequency and severity, there is an increasing need for high-resolution satellite products that accurately predict reef thermal environments over large spatio-temporal scales. Deciding which global sea surface temperature (SST) dataset to use for research or management depends on desired spatial resolution. Here, we evaluate two SST datasets – the lower-resolution CoralTemp v3.1 (0.05° ~5km grid) and the Multiscale Ultra-high Resolution MUR v4.1 (0.01° ~1km grid) – in their ability to predict in situ reef thermal environments (nightly mean and daily maximum) and the severity of past bleaching in Palau, western Pacific Ocean. We expected higher-resolution data to improve prediction accuracy, yet CoralTemp data explained 10% additional variability of in situ temperatures and 70% additional variance in past bleaching. This likely relates to differential data protocols; despite MUR using a higher spatial resolution grid, CoralTemp achieves higher raw satellite observation density in coastal areas by utilising geostationary satellites. MUR SSTs were also consistently more variable. These results reinforce calls to develop more accurate high-resolution SST products for coral reefs. Our paper demonstrates that higher spatial resolution SST data is not necessarily better at predicting in situ thermal environments and local marine heatwave impacts.
CO2 adsorption on a water-resist HKUST-1 by incorporation of Graphene Oxide
Authors: Loera-Serna Sandra , Cortés-Suárez Jonathan , Sanchez-Salas Roque , Ramírez-Rosales D. , Oliver-Tolentino M. , Ramos-Fernández Enrique V. 
Published: November 2024

Abstract

This paper aims to improve the aqueous stability and electrochemical activity of HKUST-1 (or Cu3(BTC)2, BTC = benzene 1,3,5-tricarboxylate; and HKUST from Hong Kong University of Science and Technology) by the incorporation of Graphene Oxide (GO). The synthesis was carried out in two steps; first, CuII ions were pre-adsorbed on the surface of GO, and then the BTC organic linker was added to form the HKUST-1 structure dispersed on GO sheets. Two concentrations of copper were used in the synthesis, 3.57 and 14.27 mmol to obtain the samples: GO@HKUST-1low and GO@HKUST-1high, respectively. N2 adsorption properties of GO@HKUST-1high suggest an increase in surface area compared to HKUST-1 up to 1082.0 m2·g-1. In addition, the CO2 capture of GO@HKUST-1high increased from 5.34 (HKUST-1) to 6.92 mmol·g-1 at 273 K and 100 kPa. This improvement is associated with the dispersion of the HKUST-1 on the GO sheets achieved through the synthesis strategy used, which also increased the surface area, H2O adsorption capacity of the composite material, and electrochemical stability. After the H2O adsorption tests, XRD confirmed that the material was stable under aqueous conditions, showing that the material did not undergo any structural modification.
Gene clusters-based pathway enrichment analysis identifies four pan-cancer subtypes with distinct molecular and clinical features
Authors: Xu Mengli , Ai Hongjing , Wang Danni , Wang Xiaosheng 
Published: November 2024

Abstract

Background
Pathways-based clustering methods have been proposed to explore tumor heterogeneity. However, such methods are currently disadvantageous in that specific pathways need to be explicitly claimed.
Methods
We developed the PathClustNet algorithm, a pathway-based clustering method to identify cancer subtypes. This method first detects gene clusters and identifies overrepresented pathways associated with these gene clusters. Based on the enrichment scores of the pathways, reveals cancer subtypes by clustering analysis. We applied the method to TCGA pan-cancer to identify cancer subtypes.
Results
We identified four pan-cancer subtypes, termed C1, C2, C3 and C4. C1 exhibited high metabolic activity, favourable survival and the lowest TP53 mutation rate. C2 had high immune, developmental, and stromal pathway activities, the lowest tumor purity and intratumor heterogeneity. C3 overexpressed cell cycle and DNA repair pathways were most genomically instable and had the highest TP53 mutation rate. C4 overrepresented neuronal pathways, with the lowest response rate to chemotherapy, and the highest tumor purity and genomic stability. Furthermore, ages showed positive correlations with most pathways but a negative correlation with neuronal pathways. Smoking, Viral infections and alcohol use variously affect neuron, cell cycle, immune, stromal, developmental, and metabolic pathway activities.
Conclusion
The novel PathClustNet clustering algorithm unveils a new classification of pan-cancer based on metabolic, immune, stromal, developmental, cell cycle, and neuronal pathways. These subtypes display different molecular and clinical features to warrant the investigation of precision oncology.
Efficient charge separation in Z-scheme heterojunctions induced by chemical bonding-enhanced internal electric field for promoting photocatalytic conversion of corn stover to C1/C2 gases
Authors: Gao Guoyang , Dai Yuxin , Lin Ying , Qi Houjuan , Huang Zhanhua 
Published: November 2024

Abstract

The direct conversion of corn stover into high value-added C1/C2 gases using photocatalysis is a challenging and prospective endeavor. In this work, a sulfur/oxygen dual-vacancies CdS/Co3O4 (CdS-Sv/Co3O4-Ov) Z-scheme heterojunction was designed for direct raw corn stover powder (RCSP) conversion in a photoreactive system. The internal electric field (IEF) formed in CdS-Sv/Co3O4-Ov can effectively promote the photogenerated charge separation and transfer, and the chemical bond formed at the heterogeneous interface can be used as a channel for the directional migration of photogenerated charges to accelerate the inter-interface charge transfer. Experimental results combined with DFT calculations confirmed the formation of Z-scheme heterojunction and IEF. The results of the photocatalytic RCSP reaction showed that the CO, CH4, C2H6, and C2H4 production rates of the proposed catalytic system were as high as 691.99, 2057.69, 202.93 and 187.29 µmol/g, with the corresponding CH4 selectivity and total hydrocarbon selectivity of 65.53% and 77.96%, respectively. What’s more, we propose a photocatalytic reaction mechanism in which raw biomass undergoes depolymerization and cascading oxidation to high value-added products. This study provides a new idea for high-performance photocatalytic direct conversion of RCSP into high-value-added C1/C2 gases through the rational design of photocatalysts and reaction systems.
Skin regeneration underlies cancer resistance in Acomys
Authors: White Andrew , Donahue Leanne , Hsu Chia-Hsin , Yang Youngeun 
Published: November 2024

Abstract

Cancer and regeneration share common characteristics, including cell dedifferentiation, proliferation, migration, and immune cell activation. These processes proceed, however, during tumorigenesis without the strict control exhibited during regeneration4. Most common in animals without an advanced adaptive immune system, regeneration is not widespread in mammals. The Spiny Mouse (Acomys spp.) is a notable exception, possessing the unique ability to regenerate large wounds in the skin, ear, and other complex organs completely and without the presence of scar tissue. Here, we employ Acomys to test the hypothesis that enhanced mammalian regeneration significantly alters cancer incidence and/or initiation compared to the non-regenerative laboratory mouse (Mus musculus). We found that Acomys has drastically reduced tumor incidence and increased survival following chemical carcinogenesis compared to Mus, despite a prolonged exposure period. Acomys undergoes a regenerative response including widespread cell death and increased epithelial cell proliferation in response to chemical carcinogenesis. During this process, we identify a subset of treatment-dependent Tenascin C (Tnc)-expressing regenerative fibroblasts and Tumor necrosis factor (Tnf) expressing T cells as the likely drivers of regeneration. Finally, we found that Erk phosphorylation, a key molecular mechanism of tissue regeneration in Acomys, is a significant mediator of epidermal cell death following carcinogenesis. These findings establish a new perspective on cancer initiation and the mechanistic link between tissue regeneration and intrinsic cancer resistance.
Hemoadsorption therapy for calcium channel blocker (CCB) overdose at a tertiary-level Intensive care unit in South Africa. A retrospective study
Authors: Omar Shahed , Shukla Varada , Khan Ayesha , Dangor Ziyaad 
Published: November 2024

Abstract

In this retrospective study, we describe the admission frequency, outcomes and management of adult participants with clinically confirmed CCB overdose admitted to the ICU from 2020 to 2022. Of 1719 ICU admissions over the study period, 24 (1,4%) had CCB overdose with a case fatality rate of 12.5% (3/24). Interventions included mechanical ventilation (MV) (71%), vasopressors (92%), high-dose euglycemic therapy (HIET) (71%), calcium (42%), methylene blue (4%) and fluid therapy (100%). Thirteen patients (54%) received hemoadsorption therapy (HA), and eleven received standard care (SoC) based on current guidelines. The HA group had a higher SAPS II score (p=0.002), and a greater total maximal vasopressor dose (p=0.001) compared to SoC. The HA group also had a lower admission mean arterial pressure (MAP), (p=0.014), a greater MAP increase at 48 hours (p=0.044) and a longer ICU length of stay (LOS) (p=0.004) vs. SoC. There was one death in the HA group (7.7%) and two in the SoC group (18.2%). CCB overdose is a life-threatening contributor to toxicology admissions in the ICU. Hemoadsorption therapy may provide a safe and important rescue therapy in cases with refractory shock. Well-designed studies are required to confirm its role in enhancing drug clearance and clinical outcomes.
Using Copulas to Model Random Coefficients in Panel Data Settings
Authors: Zimmer David 
Published: November 2024

Abstract

This paper considers nonlinear panel data models with random intercepts and random slopes. Existing implementations of such models always assume joint normality for random intercepts and slopes. This paper demonstrates that, in certain situations, linking the intercepts and slopes via copula functions potentially offers improved fits and more accurate estimates of treatment effects. To demonstrate, a case study investigates the effect of mental health problems on visits to outpatient departments. Normal-based and copula-based models both find positive effects. But results also show that, at the 85th percentile, a better-fitting copula-based model uncovers an effect more than 30 percent larger than its normal-based counterpart.
JEL Codes: C23; C51; I12
A Cross-sectional Study on Posttraumatic Growth and Influencing Factors among Parents of Premature Infants
Authors: Wang Xingyanan , Xiao Zhitiao , Lv yuanhong , Liu yang 
Published: November 2024

Abstract

Objective
Preterm birth is a significant public health concern that negatively affects parents' mental health. Posttraumatic growth (PTG) is a positive response to trauma that can effectively help parents cope with the stress of preterm birth. However, few studies have investigated PTG among parents with preterm infants. This study aimed to examine the level and influencing factors of PTG among parents with preterm infants and to explore its relationship with social support coping styles.
Methods
A cross-sectional study was conducted among 160 parents of premature infants in a hospital in Shenzhen, China, from May 2022 to August 2022. Parents' demographic information and birth-related characteristics were collected through a self-designed general information questionnaire. PTG was assessed using the Chinese version of the Posttraumatic Growth Inventory (PTGI). Social support and coping style were measured using the Social Support Scale(SSS) and the Ways of Coping Questionnaire(WCQ). Pearson correlation analysis was used to investigate the relationship between PTGI and WCQ and SSS .A stepwise multiple linear regression analysis was performed to explore independent influencing factors of PTG.
Results
The parents had an average PTG score of 66.41 ± 10.37, with 81.11% having a low and medium level of PTG. The total score of coping style of parents of premature infants was (34.28 ± 4.97) points, which was in the middle and upper level; the total score of social support of parents of premature infants was (14.28 ± 4.97) points, which was in the middle level. In addition, older gestational age at birth (B = 2.396, P < 0.001) ,higher Apgar score at 5 minutes at birth (B = 2.117, P = 0.023), positive coping style (B = 0.850, P < 0.001), and higher social support score (B = 1.978, P < 0.001) were associated with higher PTG scores.
Conclusion
Parents of preterm infants have low to medium levels of PTG, indicating much room for improvement. Older gestational age ,higher Apgar score at five minutes at birth, positive coping styles, and high levels of social support were associated with higher PTG levels. Our findings provide helpful guidance for the development of effective and targeted intervention programs to improve parents' PTG and well-being.
Comparative Analysis of S100β Expression on Spinal Cord Tissue of Injured Vs. Uninjured Rat Models (Rattus Norvegicus Sprague-Dawley)
Authors: Nasution Uji Kuatro , Setyono Hanis , Yarsa Kristanto Yuli , Setyawan Novan Adi 
Published: November 2024

Abstract

Background: S100β is a Ca2+-binding protein found in glial astrocytes of the central nervous system. It is known to increase in various pathological conditions, including spinal cord injury (SCI), and is commonly detected in cerebrospinal fluid and serum. However, no study has examined histopathological S100β expression in spinal cord tissue after SCI.
Objective:This study aimed to assess differences in S100β expression in spinal cord tissue of rat (Rattus norvegicus Sprague-Dawley) SCI models compared to uninjured controls.
Method: An experimental post-test only randomized control group design was used. Thirty Sprague-Dawley rats were divided into three groups: control (K), SCI induction with termination at 6 hours (P1), and SCI induction with termination at 12 hours (P2). S100β expression was analyzed using one-way ANOVA and post-hoc Tukey HSD tests.
Results: Significant differences in S100β expression were found among the groups (p = 0.006). Post-hoc analysis showed a significant increase in S100β expression in the P1 group compared to the control group (p = 0.005). No significant differences were observed between the control and P2 groups (p = 0.480) or between P1 and P2 (p = 0.077).
Conclusion: S100β expression increases significantly 6 hours after SCI but decreases by 12 hours, approaching control levels. This suggests that astrocyte activity and cell destruction are most prominent early after injury, with recovery occurring over time.