bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of a Hybrid Statistical–Dynamical System to Seasonal Prediction of North American Temperature and Precipitation
Authors: Strazzo Sarah , Collins Dan C. , Schepen Andrew , Wang Q. J. , Becker Emily , Jia Liwei 
Published: December 2018

Abstract
Recent research demonstrates that dynamical models sometimes fail to represent observed teleconnection patterns associated with predictable modes of climate variability. As a result, model forecast skill may be reduced. We address this gap in skill through the application of a Bayesian postprocessing technique—the calibration, bridging, and merging (CBaM) method—which previously has been shown to improve probabilistic seasonal forecast skill over Australia. Calibration models developed from dynamical model reforecasts and observations are employed to statistically correct dynamical model forecasts. Bridging models use dynamical model forecasts of relevant climate modes (e.g., ENSO) as predictors of remote temperature and precipitation. Bridging and calibration models are first developed separately using Bayesian joint probability modeling and then merged using Bayesian model averaging to yield an optimal forecast. We apply CBaM to seasonal forecasts of North American 2-m temperature and precipitation from the North American Multimodel Ensemble (NMME) hindcast. Bridging is done using the model-predicted Niño-3.4 index. Overall, the fully merged CBaM forecasts achieve higher Brier skill scores and better reliability compared to raw NMME forecasts. Bridging enhances forecast skill for individual NMME member model forecasts of temperature, but does not result in significant improvements in precipitation forecast skill, possibly because the models of the NMME better represent the ENSO–precipitation teleconnection pattern compared to the ENSO–temperature pattern. These results demonstrate the potential utility of the CBaM method to improve seasonal forecast skill over North America.
Using a Multiyear Temporal Climate-Analog Approach to Assess Climate Change Impacts on Park Visitation
Authors: Hewer Micah J. , Gough William A. 
Published: December 2018

AbstractBecause of the perceived weather sensitivity of park visitation in Ontario, Canada, several previous assessments have examined the impact of climate change. However, these assessments have predominantly been based on modeling approaches (regression analysis). The current study uses a multiyear temporal climate-analog approach to reassess the impact of climate change on visitation to Pinery Provincial Park in southwestern Ontario based on the observed effects of historical climatic anomalies on park visitation from 2000 to 2016. Consideration was also given to major events such as the North American terror attacks on 11 September 2001 and the confounding effect that events such as this may have had on the results. There were no statistically significant relationships (at the 95% confidence level) between seasonal climatic anomalies and park visitation in Ontario during the winter or spring seasons. There was a weak statistical relationship between anomalously warm summer seasons and park visitation, when compared to summer seasons with climatically normal temperatures; however, the presence of nonclimatic variables may have confounded these results, producing a false positive. Autumn-season park visitation was most sensitive to climatic anomalies, with the warmest temperatures causing visitation to increase by 37%, the wettest conditions causing visitation to decrease by 11%, and the driest conditions resulting in a 24% increase. These observed seasonal temperature anomalies represent temporal climate analogs for projected climate change across the span of the twenty-first century. Thus, the results of this study suggest that previous assessments may have overestimated the positive impacts of projected climate change on park visitation in this region.
Practice variations in salivary gland imaging and utility of virtual unenhanced dual energy CT images for the detection of major salivary gland stones
Authors: Beland Benjamin , Levental Mark , Srinivasan Ashok , Forghani Reza 
Published: December 2018

Background There are variations in computed tomography (CT) protocols used for evaluating sialolithiasis, with some protocols including acquisitions before and after IV contrast administration. Dual-energy CT (DECT) can be used to generate virtual unenhanced (VUE) images, potentially precluding the need for an uninfused scan. Purpose In this study, we performed a survey in order to assess variations in the imaging approach for sialolithiasis and evaluated the accuracy of DECT VUE images for the detection of salivary stones. Material and Methods Practice variations were evaluated by an online survey of the membership of the American Society of Neuroradiology. We then identified 28 patients with salivary gland calcifications matched with 28 negative controls that had both an unenhanced and a contrast-enhanced acquisition performed as DECT. A total of 123 major salivary gland calcifications and 85 tonsilloliths were evaluated and the true unenhanced series was used as gold standard. Results The survey revealed substantial variations in CT protocols used for sialolithiasis. On a per-patient basis, DECT VUE had 96.4% sensitivity and 100% specificity. On a per-calcification basis, sensitivity and specificity was 100% for stones > 2 mm but dropped for smaller calcifications. The false-negative cases corresponded to clinically insignificant, intra-glandular parotid calcifications. Inter-reader agreement was excellent (0.9256). Conclusion This study confirms that there are significant variations in CT protocols used for evaluation of sialolithiasis. A single contrast-enhanced DECT acquisition with reconstruction of VUE may represent an attractive, streamlined alternative and enable the elimination of the true unenhanced phase and associated radiation exposure.
Patient-specific design process and evaluation of a hip prosthesis femoral stem
Authors: Abdelaal Osama , Darwish Saied , El-Hofy Hassan , Saito Yoshio 
Published: December 2018

Introduction: There are several commercially available hip implant systems. However, for some cases, custom implant designed based on patient-specific anatomy can offer the patient the best available implant solution. Currently, there is a growing trend toward personalization of medical implants involving additive manufacturing into orthopedic medical implants’ manufacturing. Methods: This article introduces a systematic design methodology of femoral stem prosthesis based on patient’s computer tomography data. Finite element analysis is used to evaluate and compare the micromotion and stress distribution of the customized femoral component and a conventional stem. Results: The proposed customized femoral stem achieved close geometrical fit and fill between femoral canal and stem surfaces. The customized stem demonstrated lower micromotion (peak: 21 μm) than conventional stem (peak: 34 μm). Stress results indicate up to 89% increase in load transfer by conventional stem than custom stem because the higher stiffness of patient-specific femoral stem proximally increases the custom stem shielding in Gruen’s zone 7. Moreover, patient-specific femoral stem transfers the load widely in metaphyseal region. Conclusion: The customized femoral stem presented satisfactory results related to primary stability, but compromising proximo-medial load transfer due to increased stem cross-sectional area increased stem stiffness.
Bloodstream infections during post-cardiotomy extracorporeal membrane oxygenation: Incidence, risk factors, and outcomes
Authors: Silvetti Simona , Ranucci Marco , Pistuddi Valeria , Isgrò Giuseppe , Ballotta Andrea , Ferraris Laurenzia , Cotza Mauro 
Published: December 2018

Introduction: Veno-arterial extracorporeal membrane oxygenation after heart surgery is a relatively common procedure. It is easily applicable but associated with a number of complications, including bloodstream infections. The aim of this study is to determine the current rate and the risk factors related to bloodstream infections acquired during post-cardiotomy veno-arterial extracorporeal membrane oxygenation. Methods: Single-center retrospective study. From the overall population receiving any kind of extracorporeal membrane oxygenation from March 2013 through December 2017, the post-cardiotomy patient population was extracted, with a final sample of 92 veno-arterial extracorporeal membrane oxygenations. The risk of developing bloodstream infections as a function of extracorporeal membrane oxygenation exposure was analyzed with appropriate statistical analyses, including a Kaplan–Meier analysis. Results: Overall, 14 (15.2%) patients developed a bloodstream infection during extracorporeal membrane oxygenation or within the first 48 h after extracorporeal membrane oxygenation removal. The total extracorporeal membrane oxygenation duration in the population was 567 days, and the incidence of bloodstream infections was 24.7 bloodstream infections/1000 extracorporeal membrane oxygenation days. There was a progressive increase in the cumulative hazard ratio during the first 7 days, reaching a value of 20% on day 7; from day 7 and day 15, the hazard ratio remained stable, with a second increase after day 15. The independent risk factors associated with bloodstream infections were adult age, pre-implantation serum total bilirubin level, and the amount of chest drain blood loss. Discussion: Infections acquired during veno-arterial extracorporeal membrane oxygenation are common. Identify the risk factors that may improve strategies for treatment and prevention.
A Randomized Controlled Trial of Animal-Assisted Therapy as an Adjunct to Intensive Family Preservation Services
Authors: Flynn Erin , Roguski Julia , Wolf Julie , Trujillo Kate , Tedeschi Philip , Morris Kevin N. 
Published: December 2018

This study examined the effects of animal-assisted therapy delivered as an adjunct to standard-of-care intensive family preservation services, compared with usual care alone. Method: Families referred by Child Protective Services ( M child age = 6 years ± 4; M parent age = 32 years ± 8.26) were individually randomized to experimental ( n = 14) or control ( n = 14) intervention. Family functioning outcomes were measured using the North Carolina Family Assessment Scale for Reunification. Results: All four targeted family functioning outcomes were significantly increased for participants who received animal-assisted therapy as an adjunct to intensive family preservation services ( n = 14) with medium to large effect sizes. These improvements were sustained in two of the subscales through discharge. No significant differences were measured for the distal clinical outcome of disposition of children at discharge. Conclusions: Findings suggest that adding animal-assisted therapy as an adjunct can improve evidence-based clinical interventions aimed at enhancing the caregiving contexts of children.
Methicillin-Resistant Staphylococcus aureus and Other Multidrug-Resistant Colonizations/Infections in an Intensive Care Unit: Predictive Factors
Authors: Ochotorena Elena , Hernández Morante Juan José , Cañavate Rubén , Villegas Roberto Andrés , Viedma Inmaculada 
Published: December 2018

Introduction and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is the most prevalent pathogen causing nosocomial infections in hospitals and health centers. This work is an effort to understand the epidemiology of MRSA and other multidrug-resistant pathogens in an intensive care unit (ICU) and to analyze characteristics that might determine the risk of MRSA colonization/infection in this unit. Method: An observational, 1-year prospective longitudinal study was conducted to obtain information about MRSA and other multidrug-resistant colonizations/infections. The study was conducted with ICU patients with an artificial airway. Data were obtained from the National Study of the Control of Nosocomial Infections in Intensive Care Units database. Results: MRSA colonization was highly prevalent (33%); however, other pathogens like gram(−) Bacillus showed a higher infectious potency. Acute Physiology and Chronic Health Evaluation (APACHE-II) score >15 and hospital stay of >4 days were the main variables that significantly predicted the risk of developing MRSA colonization ( p < .001 in both cases). Moreover, the presence of MRSA increased the risk of developing a second multidrug-resistant colonization/infection, especially with methicillin-resistant Pseudomona. Discussion: The high prevalence of MRSA emphasizes the need to continue studying risk factors for MRSA colonization/infection, which may allow early identification of this pathogen. Therefore, we propose the use of the APACHE-II score and length of hospital stay to predict increased risk of MRSA colonization. Awareness of the heightened risk in particular patients could lead to early detection and prevention.
Detrimental Association of Hypogammaglobulinemia With Chronic Lung Allograft Dysfunction and Death Is Not Mitigated by On-Demand Immunoglobulin G Replacement After Lung Transplantation
Authors: Lichvar Alicia B. , Ensor Christopher R. , Zeevi Adriana , Morrell Matthew R. , Pilewski Joseph M. , Hayanga J. W. Awori , D’Cunha Jonathan , McDyer John F. , Petrov Andrej A. 
Published: December 2018

Background: Hypogammaglobulinemia (HGG), immunoglobulin G (IgG) <700 mg/dL, is associated with infections, chronic lung allograft dysfunction, and death following lung transplantation. This study evaluates the use of on-demand intravenous IgG in lung transplant recipients with HGG. Materials and Methods: This single-center retrospective cohort study of adult lung recipients evaluated 3 groups, no, untreated (u), or treated (t) HGG at first IgG administration or a matched time posttransplant. Primary outcome was freedom from allograft dysfunction. Secondary outcomes included development of advanced dysfunction, rejection, infection burden, and mortality. Results: Recipients included 484 (no HGG: 76, uHGG: 192, tHGG: 216). Freedom from chronic allograph dysfunction was highest in the non-HGG group 2 years post-enrollment (no HGG 77.9% vs uHGG 56.4% vs tHGG 52.5%; P = .002). Freedom from advanced dysfunction was significantly different 2 years post-enrollment (no HGG 90.5% vs uHGG 84.7% vs tHGG 75.4%; P = .017). Patients without HGG and those with uHGG had less mortality at 2 years post-enrollment (no HGG 84.2% vs uHGG 81.3% vs tHGG 64.8%; P < .001). Gram-negative pneumonias occurred more often in the tHGG group ( P = .02). Conclusions: Development of chronic lung allograft dysfunction, patient survival, rejection burden, and key infectious outcomes in lung transplant recipients were still problematic in the context of on-demand IgG therapy. Prospective studies are warranted.
Renal Function as a Predictor of Early Transmetatarsal Amputation Failure
Authors: Ahn Junho , Raspovic Katherine M. , Liu George T. , Lavery Lawrence A. , La Fontaine Javier , Nakonezny Paul A. , Wukich Dane K. 
Published: December 2018

Chronic kidney disease (CKD) is a major concern in patients with foot disease because it is associated with high rates of neuropathy, peripheral vascular disease, and poor wound healing. The purpose of this study was to evaluate renal dysfunction as a risk factor for reamputation after initial transmetatarsal amputation (TMA). Patients who underwent a TMA were retrospectively identified in the American College of Surgeons National Surgical Quality Improvement Program database. Of 2018 patients, reamputation after TMA occurred in 4.4%. End-stage renal disease (ESRD) was associated with 100% increased odds of TMA failure (adjusted odds ratio [OR] = 2.00; 95% CI = 1.10, 3.52), 128% increased odds of major amputation (adjusted OR = 2.28; 95% CI = 1.27, 3.96), and 182% increased odds of 30-day mortality (adjusted OR = 2.82; 95% CI = 1.69, 4.64). In addition, white blood cell count >10 000/mm3 and deep infection at the time of surgery were independently associated with TMA failure. In conclusion, severe renal dysfunction is associated with TMA failure in the short-term, perioperative period. There was no incremental increase in risk of TMA failure with worsening level of renal function before ESRD. A multidisciplinary approach should be implemented in patients with CKD to prevent foot-related pathologies that may necessitate lower-extremity amputation. Levels of Evidence: Level III: Retrospective cohort study
Baicalin promotes apoptosis and inhibits proliferation and migration of hypoxia-induced pulmonary artery smooth muscle cells by up-regulating A2a receptor via the SDF-1/CXCR4 signaling pathway
Authors: Huang Xiaoying , Mao Wei , Zhang Ting , Wang Meibin , Wang Xuetao , Li Yaozhe , Zhang Lin , Yao Dan , Cai Xueding , Wang Liangxing 
Published: December 2018

Abstract
Background
Baicalin is a flavonoid compound that exerts specific pharmacological effect in attenuating the proliferation, migration, and apoptotic resistance of hypoxia-induced pulmonary artery smooth muscle cells (PASMCs). However, the underlying mechanism has not been fully elucidated yet. Although our previous studies had indicated that activation of A2aR attenuates CXCR expression, little is known about the relationship between A2aR and SDF-1/CXCR4 axis in hypoxic PASMCs. In this study, we aimed to investigate the effect of A2aR on the SDF-1/CXCR4 axis in hypoxic PASMCs, the mechanism underlying this effect, and whether baicalin exerts its protective functions though A2aR.

Methods
Rat PASMCs were cultured under normoxia/hypoxia and divided into nine groups: normoxia, hypoxia, hypoxia + AMD3100 (a CXCR4 antagonist), hypoxia + baicalin, hypoxia + negative virus, normoxia + A2aR knockdown, hypoxia + A2aR knockdown, hypoxia + CGS21680 (an A2aR agonist), and hypoxia + A2aR knockdown + baicalin. Lentiviral transfection methods were used to establish the A2aR knockdown model in PASMCs. Cells were incubated under hypoxic conditions for 24 h. Expression levels of A2aR, SDF-1, and CXCR4 were detected using RT-qPCR and western blot. The proliferation and migration rate were observed via CCK-8 and Transwell methods. Cell cycle distribution and cell apoptosis were measured by flow cytometry (FCM) and the In-Situ Cell Death Detection kit (Fluorescein).

Results
Under hypoxic conditions, levels of A2aR, SDF-1, and CXCR4 were significantly increased compared to those under normoxia. The trend of SDF-1 and CXCR4 being inhibited when A2aR is up-regulated was more obvious in the baicalin intervention group. Baicalin directly enhanced A2aR expression, and A2aR knockdown weakened the function of baicalin. SDF-1 and CXCR4 expression levels were increased in the hypoxia + A2aR knockdown group, as were the proliferation and migration rates of PASMCs, while the apoptotic rate was decreased. Baicalin and CGS21680 showed opposite effects.

Conclusions
Our data indicate that baicalin efficiently attenuates hypoxia-induced PASMC proliferation, migration, and apoptotic resistance, as well as SDF-1 secretion, by up-regulating A2aR and down-regulating the SDF-1/CXCR4 axis.
RETRACTED ARTICLE: Tspan8 and Tspan8/CD151 knockout mice unravel the contribution of tumor and host exosomes to tumor progression
Authors: Zhao Kun , Wang Zhe , Hackert Thilo , Pitzer Claudia , Zöller Margot 
Published: December 2018

Abstract

Background
The tetraspanins Tspan8 and CD151 promote metastasis, exosomes (Exo) being suggested to be important in the crosstalk between tumor and host. The contribution of Tspan8 and CD151 to host versus tumor-derived exosome (TEX) activities being not defined, we approached the questions using 3-methylcholanthrene-induced (MCA) tumors from wt, Tspan8ko, CD151ko and Tspan8/CD151 (db)ko mice, implanted into tetraspanin-competent and deficient hosts.

Methods
Tumor growth and dissemination, hematopoiesis and angiogenesis were surveyed in wild type (wt), Tspan8ko, CD151ko and dbko mice bearing tetraspanin-competent and -deficient MCA tumors. In vitro studies using tumor cells, bone marrow cells (BMC) and endothelial cells (EC) elaborated the mechanism of serum (s)Exo- and TEX-induced target modulation.

Results
Tumors grew in autochthonous and syngeneic hosts differing in Tspan8- and/or CD151-competence. However, Tspan8ko- and/or CD151ko-tumor cell dissemination and settlement in metastatic organs was significantly reduced in the autochthonous host, and less severely in the wt-host. Impaired wt-MCA tumor dissemination in the ko-host confirmed a contribution of host- and tumor-Tspan8/-CD151 to tumor cell dissemination, delivery of sExo and TEX being severely impaired by a Tspan8ko/CD151ko. Coculturing tumor cells, BMC and EC with sExo and TEX revealed minor defects in epithelial mesenchymal transition and apoptosis resistance of ko tumors. Strongly reduced migratory and invasive capacity of Tspan8ko/CD151ko-MCA relies on distorted associations with integrins and CAM and missing Tspan8/CD151-promoted recruitment of proteases. The defects, differing between Tspan8ko- and CD151ko-MCA, were rescued by wt-TEX and, less efficiently Tspan8ko- and CD151ko-TEX. Minor defects in hematopoietic progenitor maturation were based on the missing association of hematopoietic growth factors /− receptors with CD151 and, less pronounced, Tspan8. Rescue of impaired angiogenesis in ko mice by wt-sExo and promotion of angiogenesis by TEX depended on the association of Tspan8 and CD151 with GPCR and RTK in EC and tumor cells.

Conclusions
Tspan8-/CD151-TEX play central roles in tumor progression. Tspan8-/CD151-sExo and TEX contribute by stimulating angiogenesis. Tspan8 and CD151 fulfill these tasks by associating with function-relevant proteins, the additive impact of Tspan8 and CD151 relying on differences in preferred associations. The distinct Tspan8 and CD151 contributions suggest a blockade of TEX-Tspan8 and -CD151 promising for therapeutic intervention.
The absence of retinal input disrupts the development of cholinergic brainstem projections in the mouse dorsal lateral geniculate nucleus
Authors: Sokhadze Guela , Seabrook Tania A. , Guido William 
Published: December 2018

Abstract
Background
The dorsal lateral geniculate nucleus (dLGN) of the mouse has become a model system for understanding thalamic circuit assembly. While the development of retinal projections to dLGN has been a topic of extensive inquiry, how and when nonretinal projections innervate this nucleus remains largely unexplored. In this study, we examined the development of a major nonretinal projection to dLGN, the ascending input arising from cholinergic neurons of the brainstem. To visualize these projections, we used a transgenic mouse line that expresses red fluorescent protein exclusively in cholinergic neurons. To assess whether retinal input regulates the timing and pattern of cholinergic innervation of dLGN, we utilized the math5-null (math5−/−) mouse, which lacks retinofugal projections due to a failure of retinal ganglion cell differentiation.

Results
Cholinergic brainstem innervation of dLGN began at the end of the first postnatal week, increased steadily with age, and reached an adult-like pattern by the end of the first postnatal month. The absence of retinal input led to a disruption in the trajectory, rate, and pattern of cholinergic innervation of dLGN. Anatomical tracing experiments reveal these disruptions were linked to cholinergic projections from parabigeminal nucleus, which normally traverse and reach dLGN through the optic tract.

Conclusions
The late postnatal arrival of cholinergic projections to dLGN and their regulation by retinal signaling provides additional support for the existence of a conserved developmental plan whereby retinal input regulates the timing and sequencing of nonretinal projections to dLGN.