bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contrasting Responses of Midlatitude Jets to the North Pacific and North Atlantic Warming
Authors: Matsumura Shinji , Ueki Satoaki , Horinouchi Takeshi 
Published: April 2019

AbstractMidlatitude atmospheric circulation is projected to shift poleward, yet the Northern Hemisphere jet shift is absent. Competing thermodynamic responses between tropical and Arctic warming have opposing influences on the jets and increase the uncertainties in future projections. This study shows, however, that sea surface temperature (SST) warming in the midlatitude is a major driver for the future midlatitude jet. Coupled Model Intercomparison Program phase 5 models indicate different SST warming between the midlatitude oceans, which induces a weakening of the North Pacific jet and a poleward shift of the North Atlantic jet. Our atmospheric model experiments enable to quantify the relative roles of Arctic, midlatitude, and tropical warming. The competing effects of midlatitude and tropical warming play a substantial role in the future midlatitude jet, hindering any poleward shift of the North Pacific jet, whereas for the North Atlantic jet, midlatitude SST warming is likely to win the competition.
Srlp is crucial for the self-renewal and differentiation of germline stem cells via RpL6 signals in Drosophila testes
Authors: Yu Jun , Yan Yidan , Luan Xiaojin , Qiao Chen , Liu Yuanyuan , Zhao Dan , Xie Bing , Zheng Qianwen , Wang Min , Chen Wanyin , Shen Cong , He Zeyu , Hu Xing , Huang Xiaoyan , Li Hong , Shao Qixiang , Chen Xia , Zheng Bo , Fang Jie 
Published: April 2019

AbstractSelf-renewal and differentiation in germline stem cells (GSCs) are tightly regulated by the stem cell niche and via multiple approaches. In our previous study, we screened the novel GSC regulatory gene Srlp in Drosophila testes. However, the underlying mechanistic links between Srlp and the stem cell niche remain largely undetermined. Here, using genetic manipulation of the Drosophila model, we systematically analyze the function and mechanism of Srlp in vivo and in vitro. In Drosophila, Srlp is an essential gene that regulates the self-renewal and differentiation of GSCs in the testis. In the in vitro assay, Srlp is found to control the proliferation ability and cell death in S2 cells, which is consistent with the phenotype observed in Drosophila testis. Furthermore, results of the liquid chromatography-tandem mass spectrometry (LC-MS/MS) reveal that RpL6 binds to Srlp. Srlp also regulates the expression of spliceosome and ribosome subunits and controls spliceosome and ribosome function via RpL6 signals. Collectively, our findings uncover the genetic causes and molecular mechanisms underlying the stem cell niche. This study provides new insights for elucidating the pathogenic mechanism of male sterility and the formation of testicular germ cell tumor.
NASICON-type air-stable and all-climate cathode for sodium-ion batteries with low cost and high-power density
Authors: Chen Mingzhe , Hua Weibo , Xiao Jin , Cortie David , Chen Weihua , Wang Enhui , Hu Zhe , Gu Qinfen , Wang Xiaolin , Indris Sylvio , Chou Shu-Lei , Dou Shi-Xue 
Published: April 2019

AbstractThe development of low-cost and long-lasting all-climate cathode materials for the sodium ion battery has been one of the key issues for the success of large-scale energy storage. One option is the utilization of earth-abundant elements such as iron. Here, we synthesize a NASICON-type tuneable Na4Fe3(PO4)2(P2O7)/C nanocomposite which shows both excellent rate performance and outstanding cycling stability over more than 4400 cycles. Its air stability and all-climate properties are investigated, and its potential as the sodium host in full cells has been studied. A remarkably low volume change of 4.0% is observed. Its high sodium diffusion coefficient has been measured and analysed via first-principles calculations, and its three-dimensional sodium ion diffusion pathways are identified. Our results indicate that this low-cost and environmentally friendly Na4Fe3(PO4)2(P2O7)/C nanocomposite could be a competitive candidate material for sodium ion batteries.
NRG1 type I dependent autoparacrine stimulation of Schwann cells in onion bulbs of peripheral neuropathies
Authors: Fledrich Robert , Akkermann Dagmar , Schütza Vlad , Abdelaal Tamer A. , Hermes Doris , Schäffner Erik , Soto-Bernardini M. Clara , Götze Tilmann , Klink Axel , Kusch Kathrin , Krueger Martin , Kungl Theresa , Frydrychowicz Clara , Möbius Wiebke , Brück Wolfgang , Mueller Wolf C. , Bechmann Ingo , Sereda Michael W. , Schwab Markus H. , Nave Klaus-Armin , Stassart Ruth M. 
Published: April 2019

AbstractIn contrast to acute peripheral nerve injury, the molecular response of Schwann cells in chronic neuropathies remains poorly understood. Onion bulb structures are a pathological hallmark of demyelinating neuropathies, but the nature of these formations is unknown. Here, we show that Schwann cells induce the expression of Neuregulin-1 type I (NRG1-I), a paracrine growth factor, in various chronic demyelinating diseases. Genetic disruption of Schwann cell-derived NRG1 signalling in a mouse model of Charcot-Marie-Tooth Disease 1A (CMT1A), suppresses hypermyelination and the formation of onion bulbs. Transgenic overexpression of NRG1-I in Schwann cells on a wildtype background is sufficient to mediate an interaction between Schwann cells via an ErbB2 receptor-MEK/ERK signaling axis, which causes onion bulb formations and results in a peripheral neuropathy reminiscent of CMT1A. We suggest that diseased Schwann cells mount a regeneration program that is beneficial in acute nerve injury, but that overstimulation of Schwann cells in chronic neuropathies is detrimental.
Direct reciprocity and model-predictive rationality explain network reciprocity over social ties
Authors: Dercole Fabio , Della Rossa Fabio , Piccardi Carlo 
Published: April 2019

AbstractSince M. A. Nowak & R. May’s (1992) influential paper, limiting each agent’s interactions to a few neighbors in a network of contacts has been proposed as the simplest mechanism to support the evolution of cooperation in biological and socio-economic systems. The network allows cooperative agents to self-assort into clusters, within which they reciprocate cooperation. This (induced) network reciprocity has been observed in several theoreticalmodels and shown to predict the fixation of cooperation under a simple rule: the benefit produced by an act of cooperation must outweigh the cost of cooperating with all neighbors. However, the experimental evidence among humans is controversial: though the rule seems to be confirmed, the underlying modeling assumptions are not. Specifically, models assume that agents update their strategies by imitating better performing neighbors, even though imitation lacks rationality when interactions are far from all-to-all. Indeed, imitation did not emerge in experiments. What did emerge is that humans are conditioned by their own mood and that, when in a cooperative mood, they reciprocate cooperation. To help resolve the controversy, we design a model in which we rationally confront the two main behaviors emerging from experiments—reciprocal cooperation and unconditional defection—in a networked prisoner’s dilemma. Rationality is introduced by means of a predictive rule for strategy update and is bounded by the assumed model society. We show that both reciprocity and a multi-step predictive horizon are necessary to stabilize cooperation, and sufficient for its fixation, provided the game benefit-to-cost ratio is larger than a measure of network connectivity. We hence rediscover the rule of network reciprocity, underpinned however by a different evolutionary mechanism.
The potential probiotic Lactobacillus rhamnosus CNCM I-3690 strain protects the intestinal barrier by stimulating both mucus production and cytoprotective response
Authors: Martín Rebeca , Chamignon Celia , Mhedbi-Hajri Nadia , Chain Florian , Derrien Muriel , Escribano-Vázquez Unai , Garault Peggy , Cotillard Aurélie , Pham Hang Phuong , Chervaux Christian , Bermúdez-Humarán Luis G. , Smokvina Tamara , Langella Philippe 
Published: April 2019

AbstractThe gut barrier plays an important role in human health. When barrier function is impaired, altered permeability and barrier dysfunction can occur, leading to inflammatory bowel diseases, irritable bowel syndrome or obesity. Several bacteria, including pathogens and commensals, have been found to directly or indirectly modulate intestinal barrier function. The use of probiotic strains could be an important landmark in the management of gut dysfunction with a clear impact on the general population. Previously, we found that Lactobacillus rhamnosus CNCM I-3690 can protect intestinal barrier functions in mice inflammation model. Here, we investigated its mechanism of action. Our results show that CNCM I-3690 can (i) physically maintain modulated goblet cells and the mucus layer and (ii) counteract changes in local and systemic lymphocytes. Furthermore, mice colonic transcriptome analysis revealed that CNCM I-3690 enhances the expression of genes related to healthy gut permeability: motility and absorption, cell proliferation; and protective functions by inhibiting endogenous proteases. Finally, SpaFED pili are clearly important effectors since an L. rhamnosus ΔspaF mutant failed to provide the same benefits as the wild type strain. Taken together, our data suggest that CNCM I-3690 restores impaired intestinal barrier functions via anti-inflammatory and cytoprotective responses.
Sequential, but not Concurrent, Incubation of Cathepsin K and L with Type I Collagen Results in Extended Proteolysis
Authors: Parks Akia N. , Nahata Juhi , Edouard Naomi-Eliana , Temenoff Johnna S. , Platt Manu O. 
Published: April 2019

AbstractDegradation of extracellular matrix (ECM) during tendinopathy is, in part, mediated by the collagenolytic cathepsin K (catK) and cathepsin L (catL), with a temporal component to their activity. The objective of this study was to determine how catK and catL act in concert or in conflict to degrade collagen and tendon ECM during tissue degeneration. To do so, type I collagen gels or ECM extracted from apolipoprotein E deficient mouse Achilles tendons were incubated with catK and catL either concurrently or sequentially, incubating catK first, then catL after a delayed time period. Sequential incubation of catK then catL caused greater degradation of substrates over concurrent incubation, and of either cathepsin alone. Zymography showed there were reduced amounts of active enzymes when co-incubated, indicating that cannibalism, or protease-on-protease degradation between catK and catL was occurring, but incubation with ECM could distract from these interactions. CatK alone was sufficient to quickly degrade tendon ECM, but catL was not, requiring the presence of catK for degradation. Together, these data identify cooperative and conflicting actions of cathepsin mediated collagen matrix degradation by considering interactive effects of multiple proteases during tissue degeneration.
Regression models for monitoring trace metal accumulations by Faba sativa Bernh. plants grown in soils amended with different rates of sewage sludge
Authors: Eid Ebrahem M. , Alrumman Sulaiman A. , Galal Tarek M. , El-Bebany Ahmed F. 
Published: April 2019

AbstractThe present study was conducted using a pot experiment to develop regression models for the prediction of trace metal concentrations in faba bean (Faba sativa Bernh.) plants cultivated in soils amended with different rates of sewage sludge to monitor possible human health risks. The trace metal concentrations in the different tissues of faba bean showed that most of the investigated trace metals were accumulated in the plant roots rather than in the other tissues. Meanwhile, the fruits accumulated the lowest concentration of most trace metals. The trace metal concentrations of the faba bean plants had a significant positive correlation with the organic matter content and a significant negative correlation with the soil pH. Transfer of trace metals from the soil to faba bean roots indicated that Al, Cu, Pb and Zn had a transfer factor that exceeded one, whereas the TF of the investigated trace metals from the roots to the fruits did not exceed one. The daily intake rate of the investigated trace metals did not exceed one in both adults and children. On the other side, the hazard quotient of trace metals from consuming faba bean fruits had values <1 for most investigated trace metals except Al and Mn in adults and in children. It is worth mentioning that the predicted trace metal concentrations via the established regression models and measured values from the validation data set were not significantly different (P > 0.05). Therefore, these developed models will be useful for prediction of trace metals uptake by faba bean grown in soil amended with sewage sludge so possible human risks can be identified.
The protonation state of an evolutionarily conserved histidine modulates domain swapping stability of FoxP1
Authors: Medina Exequiel , Villalobos Pablo , Coñuecar Ricardo , Ramírez-Sarmiento César A. , Babul Jorge 
Published: April 2019

AbstractForkhead box P (FoxP) proteins are members of the versatile Fox transcription factors, which control the timing and expression of multiple genes for eukaryotic cell homeostasis. Compared to other Fox proteins, they can form domain-swapped dimers through their DNA-binding –forkhead– domains, enabling spatial reorganization of distant chromosome elements by tethering two DNA molecules together. Yet, domain swapping stability and DNA binding affinity varies between different FoxP proteins. Experimental evidence suggests that the protonation state of a histidine residue conserved in all Fox proteins is responsible for pH-dependent modulation of these interactions. Here, we explore the consequences of the protonation state of another histidine (H59), only conserved within FoxM/O/P subfamilies, on folding and dimerization of the forkhead domain of human FoxP1. Dimer dissociation kinetics and equilibrium unfolding experiments demonstrate that protonation of H59 leads to destabilization of the domain-swapped dimer due to an increase in free energy difference between the monomeric and transition states. This pH–dependence is abolished when H59 is mutated to alanine. Furthermore, anisotropy measurements and molecular dynamics evidence that H59 has a direct impact in the local stability of helix H3. Altogether, our results highlight the relevance of H59 in domain swapping and folding stability of FoxP1.
Bistable behaviour and medium-dependent post-translational regulation of the tryptophanase operon regulatory pathway in Echerichia coli
Authors: Orozco-Gómez David I. , Sosa-Hernández Juan Eduardo , Gallardo-Navarro Óscar Adrián , Santana-Solano Jesús , Santillán Moisés 
Published: April 2019

AbstractThe present work is aimed at studying the dynamic behaviour of the tryptopnanase (tna) operon, which encodes the proteins necessary to uptake and metabolise tryptophan to use it as a carbon source in the absence of glucose. To this end, we designed a micro-bioreactor capable of driving a bacterial culture to a stationary state. This allowed us to explore (at the single cell level) the tna operon steady-state dynamics under multiple culture conditions. Our experimental results suggest that the tna operon is bistable for a specific range of environmental tryptophan and glucose concentrations, and evidence that both reagents play a role on the activation of the enzyme in charge of metabolising tryptophan: tryptophanase (TnaA). Based on our experimental data and the already known regulatory mechanisms, we developed a mathematical model for the tna operon regulatory pathway. Our modelling results reinforce the claim that the tna operon is bistable, and further suggest that the activity of enzyme TnaA is regulated by the environmental levels of glucose and tryptophan via a common signalling pathway. Possible biological implications of our findings are further discussed.
A novel optical tracer for VMAT2 applied to live cell measurements of vesicle maturation in cultured human β-cells
Authors: Pecic Stevan , Milosavic Nenad , Rayat Gina , Maffei Antonella , Harris Paul E. 
Published: April 2019

AbstractThe islet β-cells integrate external signals to modulate insulin secretion to better regulate blood glucose levels during periods of changing metabolic demand. The vesicular monoamine transporter type 2 (VMAT2), an important regulator of CNS neurotransmission, has an analogous role in the endocrine pancreas as a key control point of insulin secretion, with additional roles in regulating β-cell differentiation and proliferation. Here we report on the synthesis and biological characterisation of a fluorescent ligand for VMAT2 suitable for live cell imaging. Staining for VMAT2 and dopamine in live β-cell cultures show colocalisation in specific vesicles and reveal a heterogeneous population with respect to cell size, shape, vesicle number, size, and contents. Staining for VMAT2 and zinc ion, as a surrogate for insulin, reveals a wide range of vesicle sizes. Immunohistochemistry shows larger β-cell vesicles enriched for proinsulin, whereas smaller vesicles predominantly contain the processed mature insulin. In β-cell cultures obtained from nondiabetic donors, incubation at non-stimulatory glucose concentrations promotes a shift in vesicle diameter towards the more mature insulin vesicles at the expense of the larger immature insulin secretory vesicle population. We anticipate that this probe will be a useful reagent to identify living β-cells within complex mixtures for further manipulation and characterisation.
Survey of 17 elements, including rare earth elements, in chilled and non-chilled cauliflower cultivars
Authors: Kalisz Andrzej , Sękara Agnieszka , Smoleń Sylwester , Grabowska Aneta , Gil Joanna , Komorowska Monika , Kunicki Edward 
Published: April 2019

AbstractThis study investigated if genetic diversity among cauliflower cultivars (white ‘Xenia’ F1, green ‘Vitaverde’ F1, purple ‘Graffiti’ F1, orange ‘Sunset’ F1, romanesco ‘Celio’ F1) and transplant chilling are reflected in the content of 17 elements in mature curds. Transplants at 40 days after sowing were exposed to 4 °C (chilling) and 18 °C (control) for 7 days and then planted in the field till harvest maturity. The lowest Ag, Al, Co, and Li contents were found in ‘Celio’ F1cauliflower, which also had the highest Ba and Sr levels. Orange curds of ‘Sunset’ F1were the richest in Al, and high in Li, Sc, and Sn. Chilling applied to the transplants increased Ag, Ba, Co, Sc, Sr, and Tb, and decreased the Y content of mature curds. Transplant chilling can permanently alter plant metabolism, and subsequently may affect the mineral composition of the curds.
Neural cell adhesion molecule Negr1 deficiency in mouse results in structural brain endophenotypes and behavioral deviations related to psychiatric disorders
Authors: Singh Katyayani , Jayaram Mohan , Kaare Maria , Leidmaa Este , Jagomäe Toomas , Heinla Indrek , Hickey Miriam A. , Kaasik Allen , Schäfer Michael K. , Innos Jürgen , Lilleväli Kersti , Philips Mari-Anne , Vasar Eero 
Published: April 2019

AbstractNeuronal growth regulator 1 (NEGR1) belongs to the immunoglobulin (IgLON) superfamily of cell adhesion molecules involved in cortical layering. Recent functional and genomic studies implicate the role of NEGR1 in a wide spectrum of psychiatric disorders, such as major depression, schizophrenia and autism. Here, we investigated the impact of Negr1 deficiency on brain morphology, neuronal properties and social behavior of mice. In situ hybridization shows Negr1 expression in the brain nuclei which are central modulators of cortical-subcortical connectivity such as the island of Calleja and the reticular nucleus of thalamus. Brain morphological analysis revealed neuroanatomical abnormalities in Negr1−/− mice, including enlargement of ventricles and decrease in the volume of the whole brain, corpus callosum, globus pallidus and hippocampus. Furthermore, decreased number of parvalbumin-positive inhibitory interneurons was evident in Negr1−/− hippocampi. Behaviorally, Negr1−/− mice displayed hyperactivity in social interactions and impairments in social hierarchy. Finally, Negr1 deficiency resulted in disrupted neurite sprouting during neuritogenesis. Our results provide evidence that NEGR1 is required for balancing the ratio of excitatory/inhibitory neurons and proper formation of brain structures, which is prerequisite for adaptive behavioral profiles. Therefore, Negr1−/− mice have a high potential to provide new insights into the neural mechanisms of neuropsychiatric disorders.
Meta-Analysis of Asymmetric Dimethylarginine Concentrations in Rheumatic Diseases
Authors: Erre Gian Luca , Mangoni Arduino Aleksander , Castagna Floriana , Paliogiannis Panagiotis , Carru Ciriaco , Passiu Giuseppe , Zinellu Angelo 
Published: April 2019

AbstractRaised circulating concentrations of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase (NOS), have been reported in several rheumatic diseases (RDs). However, the strength of this relationship is unclear. Therefore, the aim of this systematic review and meta-analysis was to evaluate the magnitude and the robustness of the association between ADMA concentrations and RDs. We calculated standardized mean differences (SMD, with 95% confidence intervals, CI). Study heterogeneity was evaluated by meta-regressions and sensitivity analyses according to type of RDs, conventional cardiovascular risk factors, inflammatory markers, and type of ADMA assessment methodology. Thirty-seven studies with a total of 2,982 subjects (1,860 RDs patients and 1,122 healthy controls) were included in our meta-analysis. Pooled results showed that ADMA concentrations were significantly higher in patients with RDs than in healthy controls (SMD = 1.27 µmol/L, 95% CI 0.94–1.60 µmol/L; p < 0.001). However, the between-studies heterogeneity was high. Differences in ADMA concentrations between controls and RDs patients were not significantly associated with inflammatory markers, increasing age, lipid concentrations, body mass index, blood pressure, or methodology used to assess ADMA. Furthermore, subgroup analysis showed no difference across RDs. This meta-analysis showed that, in the context of significant between-study heterogeneity, circulating concentrations of ADMA are positively related to RDs.
Manipulating nitrogen regulation in diazotrophic bacteria for agronomic benefit
Authors: Bueno Batista Marcelo , Dixon Ray 
Published: April 2019

Abstract
Biological nitrogen fixation (BNF) is controlled by intricate regulatory mechanisms to ensure that fixed nitrogen is readily assimilated into biomass and not released to the environment. Understanding the complex regulatory circuits that couple nitrogen fixation to ammonium assimilation is a prerequisite for engineering diazotrophic strains that can potentially supply fixed nitrogen to non-legume crops. In this review, we explore how the current knowledge of nitrogen metabolism and BNF regulation may allow strategies for genetic manipulation of diazotrophs for ammonia excretion and provide a contribution towards solving the nitrogen crisis.