bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of PICALM Gene Polymorphisms with Alzheimer's Disease: Evidence from an Updated Meta-Analysis
Authors: Zeng Fang-Fang , Liu Jun , He Hong , Gao Xu-Ping , Liao Min-Qi , Yu Xiao-Xuan , Liu Yan-Hua , Zhu Sui , Jing Chun-Xia 
Published: August 2019

Background:
Previous studies have examined the roles of three polymorphisms (rs3851179,
rs541458, and rs592297) of the PICALM gene in susceptibility to Alzheimer's disease (AD) with inconclusive
findings.

Objective:
We performed a meta-analysis to explore whether these three polymorphisms in the PICALM
gene were associated with susceptibility to AD.

Methods:
Bibliographical searches were conducted in the PubMed, Embase, Web of Science, and China
National Knowledge Infrastructure (CNKI) databases. Summary Odds Ratios (ORs) with 95% Confidence
Intervals (CIs) were used to assess the strength of association in a random effects model. Potential
sources of heterogeneity were identified by subgroup and meta-regression analyses.

Results:
Twenty studies (9,017 cases and 15,448 controls) on rs3851179, 12 studies (8,077 cases and
12,022 controls) on rs541458, and 4 studies (2,106 cases and 2,234 controls) on rs592297 were considered
eligible for meta-analyses. For both rs3851179 and rs541458, the overall ORs were significant under
all genetic models with mild heterogeneity. Compared with G carriers, A carriers of rs3851179 were
associated with a decreased risk of AD (OR = 0.88; 95% CI 0.84, 0.91, P for Z-test <0.001, I2 = 0.0%).
Compared with T carriers, C carriers of rs541458 were inversely associated with AD risk (OR = 0.86;
95% CI 0.81, 0.92, P for Z-test <0.001, I2 = 39.5%). No association was observed for rs592297. Subgroup
and meta-regression analyses indicated that the protective effect of the rs541458 C allele was observed
only among Caucasians, not among Asians (P for interaction: 0.021~<0.001).

Conclusion:
rs3851179 and rs541458 appear to be associated with decreased AD risk. The null associations
for rs592297 with AD risk need further confirmation with a larger number of participants.
Antitumoral Potential of Lansbermin-I, a Novel Disintegrin from Porthidium lansbergii lansbergii Venom on Breast Cancer Cells
Authors: Montealegre-Sánchez Leonel , Gimenes Sarah N.C. , Lopes Daiana S. , Teixeira Samuel C. , Solano-Redondo Luis , de Melo Rodrigues Veridiana , Jiménez-Charris Eliécer 
Published: August 2019

Background:
Disintegrins from snake venoms bind with high specificity cell surface integrins,
which are important pharmacological targets associated with cancer development and progression.

Objective:
In this study, we isolated a disintegrin from the Porthidium lansbergii lansbergii venom and
evaluated its antitumoral effects on breast cancer cells.

Methods:
The isolation of the disintegrin was performed on RP-HPLC and the inhibition of platelet aggregation
was evaluated on human platelet-rich plasma. The inhibition of cell adhesion was also evaluated
in vitro on cultures of cell lines by the MTT method as well as the inhibition of breast cancer cell
migration by the wound healing assay. The binding of the disintegrin to integrin subunits was verified by
flow cytometry and confocal microscopy. Finally, inhibition of angiogenesis was assessed in vitro on
HUVEC cells and the concentration of VEGF was measured in the cellular supernatants.

Results:
The disintegrin, named Lansbermin-I, is a low molecular weight protein (< 10 kDa) that includes
an RGD on its sequence identified previously. Lansbermin-I showed potent inhibition of ADP and
collagen-induced platelet aggregation on human plasma and also displayed inhibitory effects on the adhesion
and migration of breast cancer MCF7 and MDA-MB 231cell lines, without affecting nontumorigenic
breast MCF-10A and lung BEAS cells. Additionally, Lansbermin-I prevented MCF7 cells to
adhere to fibronectin and collagen, and also inhibited in vitro angiogenesis on human endothelial HUVEC
cells.

Conclusion:
Our results display the first report on the antitumor and anti-metastatic effects of an RGDdisintegrin
isolated from a Porthidium snake venom by possibly interfering with α2 and/or β1-containing
integrins. Thus, Lansbermin-I could be an attractive model to elucidate the role of disintegrins against
breast cancer development.
A Validated DBS Method for Quantitation of a Novel Mutant IDH1/2 Inhibitor, Vorasidenib Using 10 μL Mice Blood: Application to a Pharmacokinetic Study in Mice
Authors: Dittakavi Sreekanth , Jat Rakesh Kumar , Mullangi Ramesh 
Published: August 2019

Background:
Vorasidenib is a pan-IDH inhibitor, undergoing clinical trials for the treatment
of acute myeloid leukemia.

Methods:
In this paper, we present the data of method validation to quantify vorasidenib in the mice
blood mice using dried blood spot (DBS) method on LC-MS/MS as per FDA bioanalytical method validation
guideline. Using methanol (enriched with internal standard) as an extraction solvent followed by
sonication, vorasidenib was extracted from DBS quality control samples, calibration curve samples and
pharmacokinetic study samples. Baseline separation of vorasidenib and the IS in a 2.0 μL injected sample
was accomplished by delivering 0.2% formic acid and acetonitrile (25:75, v/v) at a constant flowrate
(1.00 mL/min) on a C18 column. The total run time was 2.0 min. Using the transition pair of m/z
415.4→260.4 for vorasidenib and m/z 583.1→186.1 for the IS, the quantitation was performed. The
method linearity range was 1.00-3008.00 ng/mL.

Results:
The recovery of vorasidenib ranged between 71.28%-78.14% across the tested concentrations.
No matrix effect was seen. Intra- and inter-day precisions were ≤7.23% and intra- and inter-accuracies
ranged between 97.1%-107%. Vorasidenib was stable for three freeze/thaw cycles, up to 7 days at room
temperature and for one month at -80°C. Following intravenous and oral administration of vorasidenib
to mice, it was quantifiable up to 72 h. The oral bioavailability was 51.6%.

Conclusions:
All the validation parameters met the acceptance criteria as specified in the FDA regulatory
guideline. The results suggest that validated DBS method can be used for pharmacokinetic studies in
mice to characterize the pharmacokinetic parameters of vorasidenib post intravenous and oral administration.
Sensitive Detection of Levocetirizine as a new Generation Antihistamine by Stripping Square Wave Voltammetry
Authors: Ozturk Kubra , Bakirhan Nurgul K. , Ozkan Sibel A. , Uslu Bengi 
Published: August 2019

Background::
new and selective electrochemical sensor was developed for the determination
of levocetirizine dihydrochloride, which is an antihistaminic drug.

Method::
The investigation was performed by using cyclic, differential pulse and square wave voltammetric
methods on the β-cyclodextrin modified glassy carbon electrode. It is thereby planned to obtain
information about levocetirizine determination and its mechanism.

Result::
The efficiency of experimental parameters including pH, scan rate, and accumulation potential
and time on the anodic response of levocetirizine dihydrochloride was studied. By employing the developed
method and under optimized conditions, the current showed linear dependence with a concentration
in the range between 2 × 10-8 M and 6 × 10-6 M in pH 2.0 Britton Robinson (BR) buffer.

Conclusion::
The achieved limits of detection and quantification were found as 3.73 × 10-10 M and
1.24 × 10-9 M, respectively. In addition, the possibility of applying the developed sensor for real sample
analysis was investigated, so β-cyclodextrin modified glassy carbon electrode was used to determine
levocetirizine dihydrochloride in Xyzal® tablet dosage form. Finally, this sensor was successfully applied
to the real sample as a selective, simple, reproducible, repeatable electrochemical sensor.
Dimeric NGF Mimetic Attenuates Hyperglycaemia and DNA Damage in Mice with Streptozotocin-Induced Early-Stage Diabetes
Authors: Yagubova Svetlana , Zhanataev Aliy , Ostrovskaya Rita , Anisina Еlena , Gudasheva Тatiana , Durnev Аndrey , Seredenin Sergey 
Published: August 2019

Background:NGF deficiency is one of the reasons for reduced β-cells survival in diabetes. Our previous experiments revealed the ability of low-weight NGF mimetic, GK-2, to reduce hyperglycaemia in a model of advanced diabetes. The increase in DNA damage in advanced diabetes was repeatedly reported, while there were no data about DNA damage in the initial diabetes.Aim:The study aimed to establish whether DNA damage occurs in initial diabetes and whether GK-2 is able to overcome the damage.Methods:The early-stage diabetes was modelled in Balb/c mice by streptozotocin (STZ) (130 mg/kg, i.p.). GK-2 was administered at a dose of 0.5 mg/kg, i.p., subchronically. The evaluation of DNA damage was performed using the alkaline comet assay; the percentage of DNA in the tail (%TDNA) and the percentage of the atypical DNA comets (“ghost cells”) were determined.Results:STZ at this subthreshold dose produced a slight increase in glycemia and MDA. Meanwhile, pronounced DNA damage was observed, concerning mostly the percentage of “ghost cells” in the pancreas, the liver and kidneys. GK-2 attenuated the degree of hyperglycaemia and reduced the % of “ghost cells” and %TDNA in all the organs examined; this effect continued after discontinuation of the therapy.Conclusion:Early-stage diabetes is accompanied by DNA damage, manifested by the increase of “ghost cells” percentage. The severity of these changes significantly exceeds the degree of hyperglycaemia and MDA accumulation. GK-2 exerts an antihyperglycaemic effect and attenuates the degree of DNA damage. Our results indicate that the comet assay is a highly informative method for search of antidiabetic medicines.
Role of Moderators on Engagement of Adolescents With Depression or Anxiety in a Social Media Intervention: Content Analysis of Web-Based Interactions
Authors: Windler Carolyn , Clair Maeve , Long Cassandra , Boyle Leah , Radovic Ana 
Published: August 2019

Background
The Supporting Our Valued Adolescents (SOVA) intervention aims to use a moderated social media website to encourage peer discussion about negative health beliefs, which may prevent treatment uptake. Web moderators with a background in behavioral health are used to facilitate peer conversation to promote a sense of community, provide social support, and ensure safety.

Objective
Although moderation is a core component of this intervention, little is known on best practices for moderators to ensure safety while encouraging engagement. This study sought to describe interactions between moderators and peer users and understand moderator experiences through individual interviews.

Methods
Adolescents and young adults aged 14 to 26 years with depression or anxiety history were recruited for a usability study of the SOVA intervention. During this study, 14 moderators were trained to regularly review comments to blog posts for safety, facilitate conversation, and correct misinformation. A total of 110 blog posts and their associated comments were extracted and coded using a codebook based on items from the supportive accountability model and a peer social support analysis. Closing interviews with 12 moderators assessing their experience of moderating were conducted, recorded, and transcribed. Blog post text and comments as well as transcripts of moderator interviews were assessed using a thematic analysis approach, and blog posts were examined for trends in content of moderator comments comparing blog posts with differences in comment contributor order.

Results
There were no safety concerns during the study, and moderators only intervened to remove identifiable information. Web moderators exhibited elements of supportive accountability (such as being perceived as experts and using verbal rewards as well as offering informational and emotional support). When the moderators provided the last comment under a blog post, thereby potentially ending contribution by users, they were at times found to be commenting about their own experiences. Moderators interviewed after completing their role expressed challenges in engaging users. A cohort of moderators who received more extensive training on supportive accountability and peer social support felt their ability to engage users improved because of the training.

Conclusions
Moderators of a Web-based support site for adolescents with depression or anxiety were able to ensure safety while promoting user engagement. Moderators can elicit user engagement by offering gratitude and encouragement to users, asking users follow-up questions, and limiting their own opinions and experiences when responding to comments.
<b>USO DEL HÁBITAT, DINÁMICA POBLACIONAL Y ESTACIONALIDAD REPRODUCTIVA DE ROEDORES EN EL ALTIPLANO POTOSINO, MÉXICO
Authors: Mellink Bijtel Eric 
Published: August 2019

RESUMEN. En este trabajo se reportan las preferencias de hábitat, dinámica poblacional y estacionalidad repoductiva de Perognathus jlavus, Chaetodipus hispidus, C. nelsoni, C. penicillatus, Dipodomys ordii, D. merriami, Liomys irroratus, Reithrodontomys fulvescens, Peromyscus maniculatus, Onychomys arenicola y Sigmodon hispidus en asociación con tres sistemas agrícolas áridos, sus bordes y sus alrededores no cultivados, en el Altiplano de San Luis Potosí, México. Al parecer, la cubierta herbácea es la característica más importante del hábitat. Los roedores presentaron dos patrones estacionales diferentes: la mayoría tuvo el nivel poblacional más alto durante el verano, aunque algunos lo presentaron en invierno. A pesar de las diferencias en la estacionalidad de la población, todos los roedores tuvieron su pico reproductivo durante el verano, relacionado en alguna forma con la época de lluvias. ABSTRACT. This work reports on the habitat preferences, population dynamics and reproductive seasonality of Perognatusflavus, Chaetodipus hispidus, C. nelsoni, C. penicillatus, Dipodomys ordii. D. merriami, Liomys irroratus, Reithrodontomys fulvescens, Peromyscus maniculatus. Onychomys arenicola and Sigmodon hispidus in association with three dry farming systems and their unfarmed neighboring areas and borders, in the San Luis Potosí Plateau, Mexico. lt was found that herbaceaous cover seemed to be the most important habitat characteristic. Rodents presented two different seasonal patterns: most had their highest populations during the summer. But some did so during the winter. Despite differences in population seasonality, all rodents had their highest reproductive rates during the summer, related in some form with the rainy season. Palabras clave: San Luis Potosí, México, roedores, dinámica poblacional.
<b>COMMUNITY STRUCTURE OF BATS ALONG AN ALTITUDINAL GRADIENT IN TROPICAL EASTERN MEXICO </b>
Authors: Navarro López Daniel , León Paniagua Livia 
Published: August 2019

ABSTRACT. The changes in species richness, relative abundance, and biomass of bats were monitored along an altitudinal gradient (800 to 2,560 m) in the Sierra Madre Oriental in eastern Mexico. In general, species richness, density, and biomass were correlated with elevation, but the insectivorous bats were more numerous at mid-elevation sites. The frugivorous bats contributed with the majority of the biomass along the gradient. One migratory species (Tadarida brasiliensis) was only detected during the summer months. It is hypothesized that the observed changes are correlated to the amount of food availability and thermoregulatory abilities of bats. RESUMEN. Los cambios en riqueza de especies, abundancia relativa y biomasa de los murciélagos fueron monitoreados a lo largo de un gradiente altitudinal (800 a 2,560 m) en la Sierra Madre Oriental en el este de México. En general, la riqueza de especies, densidad y biomasa estuvieron correlacionadas con la altitud; sin embargo, los murciélagos insectívoros fueron más numerosos en altitudes intermedias. Las especies frugívoras contribuyeron con la mayor proporción de la biomasa. Una especie migratoria (Tadarida brasiliensis) sólo fue colectada en el verano. Se sugiere que los patrones observados están correlacionados con la disponibilidad de alimento y las habilidades termorregulatorias de los murciélagos. Key words: Chiroptera, community structure, altitudinal gradients, Queretaro.
<b>DIVERSIDAD Y CONSERVACIÓN DE LOS MAMIFEROS MARINOS</b>
Authors: Torres G. Alejandro , Esquivel M. Carlos , Ceballos González Gerardo 
Published: August 2019

Resumen. En México se han registrado un total de 47 especies de mamíferos marinos distribuidos en tres órdenes: Cetacea (40 spp), Carnívora (6 spp) y Sirenia (1 spp). La mayor riqueza de especies se presenta en la costa occidental de Baja California, seguida del Golfo de California, el Golfo de México-Mar Caribe y finalmente el Pacífico Sur Mexicano. La mastofauna marina de México presenta organismos con afinidades biogeográficas muy diversas ya que se encuentran especies del Pacífico, del Atlántico Norte, pantropicales y endémicas. El análisis de la situación actual de los mamíferos marinos del país mostró que, por lo menos, siete especies se encuentran en riesgo de extinción. Se analizan los factores más importantes de riesgo en el país para cada especie. Abstract. A total of 47 species of marine mammals has been recorded from Mexico. They belong to three orders: Cetacea (40 spp). Carnívora (6 spp) and Sirenia (1 spp). The highest species richness is found on Baja California's west coast, followed by the Gulf of California, the Gulf of Mexico-Caribbean Sea and, at the en d. the southern part of the Mexican Pacific. The marine mammal fauna in Mexico has a variety of biogeographical affinities because there are species from the Pacific, the North Atlantic, pantropical, and endemic ones. An analysis of the current situation of the marine mammals in Mexico showed that at least seven species are at risk of extinction. We analyze the most important risk factors for each species in the country. Palabras clave: Distribución, mamíferos marinos, México, Cetacea. Sirenia.
<i>Eumops underwoodii</i> (Chiroptera: Molossidae) en Campeche
Authors: Bolaños Jorge , Naranjo Eduardo , Escalona Griselda , Lorenzo Consuelo 
Published: August 2019

Abstract: We document a record of Underwood's bonneted bat (Eumops underwoodii) in the state of Campeche, Mexico. The specimen was found close to a water hole called "Cacatucha" within the natural protected area of Balam-Kin, Municipality of Hopelchén. There is a previous record of the species in the central region of Peten "El Remate" in the Municipality of Calkini, Campeche. The bat captured is on the Mammals Colletion of El Colegio de la Frontera Sur, San Cristobal de las Casas, Chiapas. Key words: Underwood's bonneted bat, Balam-kin, Eumops underwoodii, Campeche. Palabras clave: murciélago mastín, Balam-kin, Eumops underwoodii, Campeche.
Nuevos registros de murciélagos para el Estado de Nayarit, México.
Authors: Arroyo-Cabrales Joaquín , Polaco Oscar J. , Wilson Don E. , Gardner Alfred L. 
Published: August 2019

RESUMEN: Basados en el estudio de ejemplares depositados en museos y colectas de campo recientes, se documenta la presencia de 19 especies de murciélagos (Mammalia: Chiroptera) para el estado de Nayarit, en el centro occidente de México. Las especies registradas pertenecen a las familias Phyllostomidae (3), Vespertilionidae (11) y Molossidae (5), de ellas once son nuevos registros para el estado. Dichas especies, sumadas a las 47 previamente conocidas, ubican a Nayarit como uno de los estados más diversos del país con el 42% de la biodiversidad chiropterofaunística.PALABRAS CLAVE: Chiroptera, México, Nayarit, nuevos registros.ABSTRACT: Based on the study of museum specimens and results from recent field collections, 19 species of bats (Mammalia, Chiroptera) are newly recorded for the state of Nayarit in west-central México. Eleven of these are new records for the state. Those species pertain to the bat families Phyllostomidae (3), Vespertilionidae (11), and Molossidae (5). Those new records, along with the previous 47 known species, give the state 42% of the bat diversity of México, making it one of the most diverse states in the country.KEY WORDS: Chiroptera, México, Nayarit, new records.
<b>LA MOSKITIA HONDUREÑA, EL LÍMITE MÁS AL NORTE DE LA DISTRIBUCIÓN ACTUAL DEL OSO HORMIGUERO GIGANTE (<i>Myrmecophaga tridactyla</i>)</b>
Authors: Portillo Reyes Hector Orlando 
Published: August 2019

RESUMENEl oso hormiguero gigante también conocido en Centro América como oso caballo (Myrmecophaga tridactyla), es una de las especies carismáticas del mundo. Se ha documentado en la literatura que los límites de la distribución más septentrional para esta especie son Belice y Guatemala. El mapa de su distribución para Centro América da inicio en toda la franja Caribe de Panamá e incluye todo el territorio de Costa Rica, Nicaragua, Honduras, El Salvador y una mínima porción de la costa del Caribe de Guatemala y Belice. Sin embargo, M. tridactyla no se registra en los últimos 100 años para Guatemala, Belice y El Salvador. En Honduras se realizaron diferentes monitoreos en la región Caribe y Moskitia registrándose fotocapturas del oso caballo únicamente en la Reserva de la Biosfera del Río Plátano y la Reserva propuesta de Rus Rus. Este análisis propone como límite septentrional de la distribución del M. tridactyla la región de la Moskitia hondureña, basados en la ausencia de esta especie en los listados actuales oficiales de los países de Guatemala, El Salvador y Belice, en la revisión bibliográfica para Centro América y en los monitoreos que se realizaron en la región Caribe y Moskitia hondureña. Palabras clave: Centro América, oso caballo, septentrional, Caribe, Moskitia.ABSTRACTThe giant anteater also known in Central America as oso caballo (Myrmecophaga. tridactyla), is one of the world’s charismatic species. It has been mentioned for different sources that giant anteater most northern limit distributions are Belize and Guatemala. The distribution map for Central America begins and extends throughout the Caribbean of Panama and includes the entire territory of Costa Rica, Nicaragua, Honduras, El Salvador and a minimal portion of the Caribbean coast of Guatemala and Belize. However M. tridactyla is not recorded in the last 100 years in Guatemala, Belize and El Salvador. In Honduras several biological monitoring were performed in the Caribbean and the Moskitia region recorded evidence for the specie only for the Biosphere Reserve of Río Plátano and the biological Reserve of Rus Rus in the Moskitia region. This analysis propose as most northerly for M. tridactyla the Honduran Moskitia region, based in the absence on the most recent check list for Guatemala, El Salvador, and Belize, also literature review for Central America and the biological monitoring performed in the Caribbean and Moskitia region. Keywords: Central America, giant anteater, Northern, Caribbean, Moskitia.