bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of lncRNA Gm2691 attenuates apoptosis and inflammatory response after myocardial infarction through PI3K/Akt signaling pathway
Authors: Li Tingting , Tian Hongbo , Li Jun , Zuo Anju , Chen Jiying , Xu Dan , Guo Yuan , Gao Haiqing 
Published: June 2019

AbstractAcute myocardial infarction is one of the most threatening disease in the world. In previous studies, numerous dysregulated lncRNAs exposed to ischemic reperfusion injury have been identified. In this differential lncRNAs, Gm2691 attracted our attention due to its high fold change. The aim of the study was to investigate the function and mechanism of lncRNA Gm2691 in ischemic reperfusion injury. AnaeroPack anaerobic system treated neonatal rat ventricular cardiomyocytes were used to analyze the function of lncRNA Gm2691 in vitro. Tunel, Caspase3, and inflammation markers were detected to evaluate apoptosis and inflammatory response. Rat acute myocardial infarction was performed to elucidate the function of lncRNA Gm2691 in vivo. The results showed that LncRNA Gm2691 improved the cardiac function and attenuated the inflammatory response in vivo. We also found that lncRNA Gm2691 reduced the apoptosis and improved cell survival rates in anaeroPack anaerobic system treated neonatal rat ventricular cardiomyocytes. Western blot analysis revealed that lncRNA Gm2691 decreased Akt and ERK1/2 activities, suggesting that lncRNA Gm2691 may functioned through Akt signaling pathway. We verified the function and mechanism of lncRNA Gm2691 and provide evidence that lncRNA Gm2691 may play important role in ischemic reperfusion injury, and understanding the precise role of Gm2691 will undoubtedly shed new light on the clinical treatment.
Volume intensified dilution of a ring‐closing metathesis in ethyl acetate by means of a membrane‐assisted process in solvent recycle
Authors: Porto‐Carrero Wim , Cupani Anna , Veenstra Marieke J , Dorbec Matthieu , Eykens Lies , Ormerod Dominic 
Published: June 2019

AbstractBACKGROUNDRing‐closing metathesis (RCM) for the synthesis of macrocycles has been used more and more often over recent years, including some interesting applications on industrial scale. However, like all macrocyclization strategies RCM is plagued by the traditional issue of low volume efficiency. To‐date this is typically addressed in a molecule specific manner with varying degrees of success. Here we report a process intensification method of metathesis macrocyclization that reduces the solvent load required for the reaction significantly.RESULTSMetathesis macrocylizations were successfully carried out in a solvent volume of upto 82% lower than an equivalent batch reaction, with only minimal impact upon the reaction outcome. A switch of reaction solvent to ethyl acetate renders the process more benign and applicable to large scale.CONCLUSIONA membrane‐assisted processing method that relies upon organic solvent nanofiltration permitting an internal solvent recycling and concumittent in situ product removal. The method is also designed to be applicable to a wide range of metathesis cyclizations. © 2019 Society of Chemical Industry
ELK1‐activated GPC3‐AS1/GPC3 axis promotes the proliferation and migration of cervical cancer cells
Authors: Hu Rong , Zhu Zongming 
Published: June 2019

AbstractBackgroundLong non‐coding RNA (lncRNA) is reported to be involved in multiple biological processes in numerous human tumors. Furthermore, an increasing number of studies have confirmed the involvement of lncRNA in the initiation and development of human cancers, including cervical cancer (CC).MethodsThe present study explored the potential role of lncRNA glypican 3 antisense transcript 1 (GPC3‐AS1) with respect to regulating CC cell proliferation and migration.ResultsA quantitative reverse transcriptase‐polymerase chain reaction confirmed that GPC3‐AS1 was up‐regulated in CC cells compared to normal CRL‐2614 cells. Loss‐of‐function assays demonstrated the negative effect of GPC3‐AS1 depletion on CC cell proliferation and migration. GPC3‐AS1 positively regulated its nearby gene glypican 3 (GPC3). Significant up‐regulation of GPC3 was also observed in CC cells, consistently with GPC3‐AS1. In addition, GPC3‐AS1 and GPC3 synergistically promoted the proliferative and migratory abilities of CC cells. Mechanistic investigation showed that ELK1 acted as the transcription activator of GPC3‐AS1 and GPC3, thus contributing to their up‐regulation in CC cells. Rescue assays indicated that the ELK1‐induced GPC3‐AS1/GPC3 axis promoted cell proliferation and migration in CC.ConclusionsThe results of the present study have revealed a novel molecular pathway that can regulate CC cell proliferation and migration, thus providing a new basis for investigating the molecular mechanism associated with CC progression.
Cationic micelle: A promising nanocarrier for gene delivery with high transfection efficiency
Authors: Wang Haili , Ding Shenggang , Zhang Ze , Wang Longhai , You Yezi 
Published: June 2019

AbstractMicelles have demonstrated an excellent ability to deliver several different types of therapeutic agents, including chemotherapy drugs, proteins, small‐interfering RNA and DNA, into tumor cells. Cationic micelles, comprising self‐assemblies of amphiphilic cationic polymers, have exhibited tremendous promise with respect to the delivery of therapy genes and gene transfection. To date, research in the field has focused on achieving an enhanced stability of the micellar assembly, prolonged circulation times and controlled release of the gene. This review focuses on the micelles as a nanosized carrier system for gene delivery, the system‐related modifications for cytoplasm release, stability and biocompatibility, and clinic trials. In accordance with the development of synthetic chemistry and self‐assembly technology, the structures and functionalities of micelles can be precisely controlled, and hence the synthetic micelles not only efficiently condense DNA, but also facilitate DNA endocytosis, endosomal escape, DNA uptake and nuclear transport, resulting in a comparable gene transfection of virus.
The CYP17A1 gene polymorphisms are associated with hypercholesterolemia in Han Chinese
Authors: Zhang Ning , Chen Huimei , Jia Jian , Ye Xiaoman , Ding Haixia , Zhan Yiyang 
Published: June 2019

AbstractBackgroundThe CYP17A1 gene has been identified to associate with hypertension in Chinese population. However, the association between CYP17A1 polymorphisms and hypertension‐related factors is unclear. The present study aimed to investigate the relation between CYP17A1 single nucleotide polymorphisms (SNPs) and serum lipid profiles.MethodsIn total, 1350 participants were included in the study. Six SNPs in or near CYP17A1 gene were genotyped in a Han Chinese population in two stages.ResultsThere was a statistically significant association of rs1004467 (adjusted odds ratio = 0.783, 95% confidence interval = 0.667–0.919, p < 0.05) and rs11191548 (adjusted odds ratio = 0.788, 95% confidence interval = 0.672–0.925, p < 0.05) with hypercholesterolemia after adjustment for potential factors. Additionally, the rs1004467 minor G‐allele and the rs11191548 minor C‐allele were significantly associated with the lower serum total cholesterol levels (p < 0.05 for all).ConclusionsThe rs1004467 and rs11191548 in the CYP17A1 gene are associated with a decreased risk of hypercholesterolemia and lower serum TC levels in Han Chinese.
Radiomics nomogram for differentiating between benign and malignant soft‐tissue masses of the extremities
Authors: Wang Hexiang , Nie Pei , Wang Yujian , Xu Wenjian , Duan Shaofeng , Chen Haisong , Hao Dapeng , Liu Jihua 
Published: June 2019

BackgroundPreoperative differentiation between malignant and benign tumors is important for treatment decisions.Purpose/HypothesisTo investigate/validate a radiomics nomogram for preoperative differentiation between malignant and benign masses.Study TypeRetrospective.PopulationImaging data of 91 patients.Field Strength/SequenceT1‐weighted images (570 msec repetition time [TR]; 17.9 msec echo time [TE], 200–400 mm field of view [FOV], 208–512 × 208–512 matrix), fat‐suppressed fast‐spin‐echo (FSE) T2‐weighted images (T2WIs) (4331 msec TR; 87.9 msec TE, 200–400 mm FOV, 312 × 312 matrix), slice thickness 4 mm, and slice spacing 1 mm.AssessmentFat‐suppressed FSE T2WIs were selected for extraction of features. Radiomics features were extracted from fat‐suppressed T2WIs. A radiomics signature was generated from the training dataset using least absolute shrinkage and selection operator algorithms. Independent risk factors were identified by multivariate logistic regression analysis and a radiomics nomogram was constructed. Nomogram capability was evaluated in the training dataset and validated in the validation dataset. Performance of the nomogram, radiomics signature, and clinical model were compared.Statistical Tests1) Independent t‐test or Mann–Whitney U‐test: for continuous variables. Fisher's exact test or χ2 test: comparing categorical variables between two groups. Univariate analysis: evaluating associations between clinical/morphological characteristics and malignancy. 2) Least absolute shrinkage and selection operator (LASSO)‐logistic regression model: selection of malignancy features. 3) Significant clinical/morphological characteristics and radiomics signature were input variables for multiple logistic regression analysis. Area under the curve (AUC): evaluation of ability of the nomogram to identify malignancy. Hosmer–Lemeshow test and decision curve: evaluation and validation of nomogram results.ResultsThe radiomics nomogram was able to differentiate malignancy from benignity in the training and validation datasets with an AUC of 0.94. The nomogram outperformed both the radiomics signature and clinical model alone.Data ConclusionThis radiomics nomogram is a noninvasive, low‐cost preoperative prediction method combining the radiomics signature and clinical model.Level of Evidence: 3Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2020;51:155–163.
The key role of decadal modulated oscillation in recent cold phase
Authors: Luo Wen , Guan Xiaodan , Xie Yongkun , Liu Jingchen , Zhou Yubin , Zhang Beidou 
Published: June 2019

AbstractGlobal temperature change is strongly affected by internal climate variability (ICV). The temporal change of the ICV on the decadal to multi‐decadal scales is referred as the decadal modulated oscillation (DMO) that plays a dominated role in the occurrence of enhanced warming and warming hiatus. However, investigation on the DMO in modern historical period has received limited attention. In this study, the ensemble empirical mode decomposition (EEMD) method was applied to the surface air temperature (SAT) during the boreal cold season to extract the DMO signal in the past century. Two most sensitive areas of DMO trend over northern Eurasia and northwestern North America were identified and used to build a time series of regionally enhanced DMO. It showed an obvious decadal periodic oscillation at 11–23 years and exhibited increasing amplitude. In addition, regression analysis using Niño3.4, Pacific Decadal Oscillation (PDO), Atlantic Multi‐decadal Oscillation (AMO), and Arctic Oscillation (AO) revealed a major role of the AO in DMO over the mid‐to‐high latitudes in the Northern Hemisphere (NH). However, such strong oscillation signal has not been detected in most of the Coupled Model Intercomparison Project Phase 5 (CMIP5) models, and the extracted regionally enhanced DMO are capable of improving the predictability of SAT over the mid‐to‐high latitudes in the NH.
Effect of smoking on the DNA methylation pattern of the SOCS1 promoter in epithelial cells from the saliva of patients with chronic periodontitis
Authors: de J.H. Martinez Cristhiam , Villafuerte Kelly R.V. , Luchiari Heloise R. , de O. Cruz Juliana , Sales Mariana , Palioto Daniela B. , Messora Michel R. , Souza Sergio L.S. , Taba Mario , Ramos Ester S. , Novaes Arthur B. 
Published: June 2019

AbstractBackgroundThe aim of the present study was to evaluate the methylation pattern in the suppressor of cytokine signaling 1 (SOCS1) gene in smokers and non‐smokers with chronic periodontitis (CP).MethodsMethylation‐specific polymerase chain reaction (PCR) was performed to determine the methylation status of the SOCS1 promoter in 45 saliva samples from smokers and non‐smokers with CP.ResultsCells from the saliva of CP patients who smoked were 7.08 times more likely to have a methylated SOCS1 promoter than cells from the saliva of non‐smoking patients.ConclusionsSOCS1 gene promoter methylation, with its potential effects on the expression of this gene, seems to be a consequence of exposure to tobacco and not to periodontal disease. Further studies are needed to elucidate the relationship between the epigenetic control of immune response gene expression, exposure to environmental factors, and the development, progression, and prognosis of CP.
Minimally invasive manganese‐enhanced magnetic resonance imaging for the sciatic nerve tract tracing used intra‐articularly administrated dextran–manganese encapsulated nanogels
Authors: Eguchi Yawara , Murayama Shuhei , Kanamoto Hirohito , Abe Koki , Miyagi Masayuki , Takahashi Kazuhisa , Ohtori Seiji , Aoki Ichio 
Published: June 2019

AbstractManganese‐enhanced magnetic resonance imaging (MEMRI) enables tract tracing to follow neural pathways through axonal transport. However, the method is problematic because of the high local concentrations of Mn2+ involved. We developed a tetrananogel containing a dextran‐manganese complex (Dex‐Mn‐Gel) and applied this nanogel to rats. MnCl2 (n = 5), Dex‐Mn‐Gel (n = 5), or saline control (n = 3) was injected into the left knee joint of the rats (n = 13). Inflammation and tissue alterations in the knee joint were also evaluated histologically. T1‐weighted images were obtained on a 7 T MRI system 24 hours after the administration and compared across groups. The sciatic nerve in both legs and the surrounding musculature were used as regions of interest (ROI). No swelling was found in the knee joint infused with Dex‐Mn‐Gel, although prominent swelling of the knee joint was observed with MnCl2. White blood cells inside the knee joint tissue infused with the Dex‐Mn‐Gel were significantly less abundant (45%, P < .05) compared with the knee joints infused with MnCl2. Visualization of the sciatic nerve was significantly enhanced in rats treated with both forms of Mn2+ compared with controls (P < .01). This study is the first to attempt intra‐articular administration of a manganese contrast agent into joint‐capsule and demonstrate tract visualization. The Dex‐Mn‐Gel can be taken up by the nerve endings and reduce Mn2+ toxicity. Dex‐Mn‐Gel will provide a minimally invasive method for visualizing nerve tracts in vivo.
Unique phenolic constituent in Cimicifuga dahurica (Turcz.) Maxim. through Box–Behnken design and response surface methodology
Authors: Yu Yang , Lv Chong‐Ning , Qin Ru‐Lan , Lv Meng‐Ya , Li Yang , Lu Jin‐Cai 
Published: June 2019

AbstractCimicifuga dahurica (Turcz.) Maxim. exerts significant antioxidative due to its high phenolic constituent content. In this study, the extraction condition of the phenolic constituents and antioxidant effect was optimized by the Box‐Behnken design and response surface methodology. Eleven main bioactive analytes of Cimicifuga dahurica (Turcz.) Maxim. were simultaneously quantified by high‐performance capillary electrophoresis with diode‐array detector to assess the effect of extraction technology. The optimal extraction parameters were determined as: the concentration of ethanol 56.21%, liquid/solid ratio 14.65:1, and extraction time 1.64 h for 2.67 times. According to the results, a maximal value of total phenolic acids (3.67 mg/g) was obtained. Meanwhile, the influence of different extraction technology on antioxidant activities were evaluated by 1,1‐diphenyl‐2‐picrylhydrazyl, 2,2′‐azino‐bis‐(3‐ethylbenzthiazoline‐6‐sulfonate), ferric reducing antioxidant power and hydroxyl radical scavenging method. The results presented here showed that the content of phenolic acid and antioxidant effect was much higher than the European Pharmacopoeia. Altogether, this method successfully applied response surface methodology to optimize the Cimicifuga dahurica (Turcz.) Maxim. extract with high antioxidant activities.
Associations Among Exposure to Potentially Morally Injurious Experiences, Spiritual Injury, and Alcohol Use Among Combat Veterans
Authors: Battles Allison R. , Kelley Michelle L. , Jinkerson Jeremy D. , C. Hamrick Hannah , F. Hollis Brittany 
Published: June 2019

AbstractPotentially morally injurious experiences (PMIEs) are events that may violate deeply held values or belief systems. Combat engagement places service members at a heightened risk for PMIE exposure. Exposure to PMIEs may elicit internal conflict between moral beliefs and experiences and, if unresolved, conflict may manifest as feelings of guilt, shame, and spiritual or existential crisis. Further, distress caused by these experiences may promote harmful behaviors (e.g., excessive alcohol use), which may serve as attempts to cope with PMIEs veterans have witnessed or participated in. The present study examined a sequential mediation model in which combat exposure was associated with alcohol use (i.e., alcohol consumption, dependence symptoms, and alcohol‐related problems) via PMIE exposure and spiritual injury (e.g., alienation from and/or anger towards respective higher power) in a community sample of 380 recent‐era combat veterans. Multiple‐group sequential mediation was then used to examine whether the model fit similarly across men and women. Exposure to PMIEs and spiritual injury sequentially mediated the association between combat and alcohol; higher levels of PMIE exposure and spiritual injury were associated with increased alcohol use, R2 = .17, f2 = 0.07. The multiple‐group model showed that these associations significantly varied between genders such that the mediation was only significant among men. The results indicated that PMIEs and spiritual injury were associated with increased alcohol use, but these associations differed as a function of gender. Future research is needed to refine our understanding of moral and spiritual injury and explore possible risk and protective factors.
Endoscopic sinus surgery for pediatric patients: Prognostic factors related to revision surgery
Authors: Wu Pei‐Wen , Huang Chien‐Chia , Yang Shih‐Wei , Huang Yenlin , Huang Chi‐Che , Chang Po‐Hung , Lee Yun‐Shien , Lee Ta‐Jen 
Published: June 2019

ObjectivesIn the past two decades, endoscopic sinus surgery (ESS) has been performed frequently in children with medically recalcitrant chronic rhinosinusitis (CRS). However, surgical success rates vary according to age. The causes of failure and prognostic factors for revision ESS remain unclear. The aims of the present study were to evaluate prognostic factors related to revision surgery after ESS, and to determine the most optimal timing for surgery in the pediatric population.MethodsThis was a retrospective review in a tertiary medical center. Children who underwent ESS for CRS between 2004 and 2017 were enrolled. Demographics, sinonasal examination findings, previous operation history, laboratory data, comorbidities, and computed tomography (CT) results were collected from medical records. Prognostic factors for revision surgery were analyzed.ResultsA total of 188 pediatric patients were enrolled in this study. Twenty‐four patients (12.8%) required revision surgery. Multivariate logistic regression analysis identified patients with nasal allergy (OR = 6.258; P = .010) and higher Lund‐Mackay score on preoperative sinus CT (OR = 1.658; P = .043) had worse outcomes, while older age was a positive prognostic factor (OR = 0.702; P = .018). A cut‐off point of 15.68 years of age and mean Lund‐Mackay score of 10.5 showed the best predictive power for revision surgery.ConclusionBecause ESS is performed with increasing frequency in children, knowledge of prognostic factors for revision surgery is important. In this study, patients with younger age, nasal allergy, and higher Lund‐Mackay score on preoperative sinus CT had worse outcomes.Level of Evidence4 Laryngoscope, 130:1051–1055, 2020
Determining the relative importance of factors affecting the success of innovations in forest technology using AHP
Authors: Blagojevic Bosko , Athanassiadis Dimitris , Spinelli Raffaele , Raitila Jyrki , Vos John 
Published: June 2019

AbstractA number of factors affect the success, or otherwise, of any technological innovation. In this paper, we consider factors that affect the success of innovations in the context of forest machinery manufacturing (FMM). We report the results of a study that seeks to determine the relative importance of 19 different factors affecting the success of innovations in the FMM sector. The first stage of the study makes use of the analytic hierarchy process to determine the relative importance (weights) of these factors, based on the responses of 15 decision makers (DMs) employed by different FMM companies. The study then considers three different ways of weighting the responses of DMs, based on different assumptions about how the responses of outliers—those DMs whose views differ significantly from the majority—should be handled. The first method assumes that the responses of all DMs are of equal value. The second assumes that the influence of outliers' responses should be reduced and makes use of a Euclidean distance metric to determine the weight that should be ascribed to each DM's responses. The third assumes that the influence of outliers' responses should be increased and makes use of hierarchical clustering techniques. Although the weight assignment methods were based on very different assumptions about how to treat the views of outliers, they produced similar rankings of factors. Results showed that for FMM companies, customer satisfaction, proportion of total sales by new products, influence on the overall profit of the company, and growth of demand for the company products were the most important factors for measuring the success of innovations.