bluuebunny/crossref_metadata_2025_split huggingface dataset:
Security Audit of The Public Sector Application Using COBIT 5 and ITIL V4 Framework
Authors: Alavio Julianti , Mawarni Rima , Minarti Sulas 
Published: October 2024

The use of information system-based applications has become commonplace in various sectors, including government agencies such as the Social Service and hundreds of villages in North Lampung. One of the applications used is SIKS-NG (Social Welfare Information System - Next Generation) which aims to facilitate the management of population data and community welfare programs. However, along with technological developments, data security is increasingly important to pay attention to. Cybercrime attacks, data leaks, and privacy violations are threats that must be taken seriously. The aim of this final assignment is to determine the extent of the security of the SIKS-NG application based on the results of COBIT 5 and ITIL V4 calculations and provide recommendations for improvements to increase information system security. The method used is the COBIT 5 and ITIL V4 method using the Align, Plan and Organize (APO13), Deliver, Support and Service (DSS05), Investment Management (IM14) domains. Data analysis uses the Capability Level, Maturity Level and Linkert Scale methods. The results of this research show that the Capability Level for the APO13 subdomain process obtained an average of 4.24 with a Gap of -0.24, DSS05 obtained an average value of 4.20 with a Gap of -0.20. From the overall results, the APO13 and DSS05 subdomain framework is at level 4 and has a Gap of -O.22, which means that at this level it shows that this subdomain has been achieved and fulfilled. Meanwhile, the Maturity Level for the IM13 subdomain process is on Scale 4 with an average value of 4.17 with a Gap of 0.83, which means it has not reached the target as expected. So this means that the security of the SIKS-NG application information system has the status of being well managed and must continue to be optimized, it's just that the program performance must be repaired and improved further.
Keywords— System Security, COBIT 5, ITIL V4, Capability Level, Maturity Level
Information System for Data Collection on Building Tools and Materials Using the Rational Unified Process (RUP) Method
Authors: Siregar Sahnas Wulandari , Putri Raissa Amanda 
Published: October 2024

UD. FLORA records the re-inventory of building equipment that has been sold by reading the transactions one by one in the ledger. A proper way is needed to overcome the problems that occur. This research aims to create an information system software. In making information system software, a method is needed that can carry out the stages from initial design to implementation of the software. This research uses the Rational Unified Process (RUP) method, namely a software development approach that is carried out repeatedly (iterative), focuses on architecture (architecture-centered), is more directed based on use cases (use case driven). This research produces a tool data collection application at UD. FLORA using the RUP method. The existence of an information system for data collection on building equipment using the Rational Unified Process method means that UD. FLORA can be helped in managing building equipment data collection. The system enables real-time transaction recording, providing better insight into inventory status, and supporting more accurate decision-making.
Enhancing wound healing through innovative technologies: microneedle patches and iontophoresis
Authors: Jin Yong Xun , Ngoc Chien Pham , Thi Nga Pham , Zhang Xin Rui , Ngan Giang Nguyen , Thi Thuy Le Linh , Trinh Thuy-Tien Thi , Zhou Shu Yi , Nam Sun Young , Heo Chan Yeong 
Published: October 2024

IntroductionWound healing is a complex process involving multiple stages, including inflammation, proliferation, and remodeling. Effective wound management strategies are essential for accelerating healing and improving outcomes. The CELLADEEP patch, incorporating iontophoresis therapy and microneedle technology, was evaluated for its potential to enhance the wound healing process.MethodsThis study utilized a full-thickness skin defect model in Sprague-Dawley rats, researchers compared wound healing outcomes between rats treated with the CELLADEEP Patch and those left untreated. Various histological staining techniques were employed to examine and assess the wound healing process, such as H&amp;E, MT and immunofluorescence staining. Furthermore, the anti-inflammatory and proliferative capabilities were further investigated using biochemical assays.ResultsMacroscopic and microscopic analyses revealed that the CELLADEEP patch significantly accelerated wound closure, reduced wound width, and increased epidermal thickness and collagen deposition compared to an untreated group. The CELLADEEP patch decreased nitric oxide and reactive oxygen species levels, as well as pro-inflammatory cytokines IL-6 and TNF-α, indicating effective modulation of the inflammatory response. Immunofluorescence staining showed reduced markers of macrophage activity (CD68, F4/80, MCP-1) in the patch group, suggesting a controlled inflammation process. Increased levels of vimentin, α-SMA, VEGF, collagen I, and TGF-β1 were observed, indicating enhanced fibroblast activity, angiogenesis, and extracellular matrix production.DiscussionThe CELLADEEP patch demonstrated potential in promoting effective wound healing by accelerating wound closure, modulating the inflammatory response, and enhancing tissue proliferation and remodeling. The CELLADEEP patch offers a promising non-invasive treatment option for improving wound healing outcomes.
Safe and favorable prognosis of thoracic endovascular aortic repair for the low-risk patients with non-acute type B aortic dissection
Authors: Nakamura Ken , Kobayashi Kimihiro , Nakai Shingo , Sho Ri , Arai Shusuke , Ishizawa Ai , Watanabe Daisuke , Hirooka Shuto , Ohba Eiichi , Mizumoto Masahiro , Kuroda Yoshinori , Kim Cholsu , Uchino Hideaki , Shimanuki Takao , Uchida Tetsuro 
Published: October 2024

ObjectivePreemptive thoracic endovascular aortic repair (TEVAR) has the potential to improve the prognosis of Stanford type B aortic dissection (TBAD), however it is important to determine whether it could be safely performed as a prophylactic treatment. This study aimed to determine the short- and long-term outcomes of preemptive TEVAR for uncomplicated TBAD with a small aortic aneurysm.DesignRetrospective multicenter analysis.MethodsWe analyzed 212 patients with medically treated uncomplicated subacute TBAD between July 2004 and October 2019 in two Japanese academic centers. The short- and long-term prognosis of patients who underwent preemptive TEVAR and the changes in aortic diameter over time after TEVAR were analyzed. Aorta-related complications, aortic-related death and postoperative complications were recorded and analyzed. Analysis was performed on an intension-to-treat basis.ResultsDuring follow-up, patients were divided into two groups: optimal medical treatment [OMT; n = 185 (87%)] and preemptive TEVAR [n = 27 (13%)]. In all cases, aortic enlargement was the reason for therapeutic intervention in the preemptive TEVAR group. Propensity score matching yielded a cohort of 27 control patients with OMT (group A) and 27 patients who underwent preemptive TEVAR (group B). Preoperative characteristics were similar between groups. In group B, only one patient developed type A dissection at a late stage and died from aortic rupture. Freedom from aortic-related death at 1/5/10 years was 100%/92%/92% in group B. Overall growth (mm/year) of max aorta was significantly smaller in the TEVAR group than in the control group (−3.7 ± 2.9 vs. 0.4 ± 5.6, p &lt; 0.01), and the diameter of the false lumen was reduced (−8 ± 4.8 vs. −1.3 ± 8.0, p &lt; 0.001).ConclusionsShort- and long-term outcomes of TEVAR for uncomplicated TBAD with a small aortic aneurysm were excellent, with few postoperative complications. After TEVAR, aortic remodeling was observed in the short term, suggesting that it may contribute to the prevention of aortic-related death due to rupture.
The inverse relationship between Life’s Essential 8 and risk of metabolic syndrome: evidence from NHANES 2005-2018
Authors: Liu Yuhang , Tang Jialing , Gao Siyao 
Published: October 2024

BackgroundMetabolic syndrome (MetS) has a close association with cardiovascular diseases. Few studies have investigated the association of Life’s Essential 8 (LE8), the updated measurement of cardiovascular health (CVH), with MetS.MethodsThe National Health and Nutrition Examination Survey (2005–2018) data was extracted. The LE8 comprised 4 health behaviors (diet, physical activity, nicotine exposure, and sleep health) and 4 health factors [body mass index (BMI), blood lipids, blood glucose, and blood pressure (BP)]. The total LE8 score is the average of 8 metric scores (0-100), categorized into low (0–49), moderate (50–79), and high CVH (80–100) levels. Multivariable logistic regression models, restricted cubic spline models and stratified analyses were performed to examine the relationship between LE8 and MetS.ResultsIn this study, a total of 21,543 participants represented 146.6 million non-institutionalized U.S. adults. Following adjustment for various potential covariates, participants who attained a moderate [adjusted odds ratio (AOR) = 0.234, 95% CI: 0.209, 0.262] or a high CVH level (AOR = 0.026, 95% CI: 0.021, 0.032) exhibited an inverse correlation with MetS risks when comparing those with a low CVH level. An inverse linear dose-response relationship between LE8 scores and MetS risks was also identified (P for nonlinearity &gt; 0.05).ConclusionsLE8 was inversely associated with the risk of MetS. Adhering to LE8 guidelines to sustain a higher CVH level may be beneficial for preventing MetS.
Computational staining of CD3/CD20 positive lymphocytes in human tissues with experimental confirmation in a genetically engineered mouse model
Authors: Li Xiang , Heirman Casey C. , Rickard Ashlyn G. , Sotolongo Gina , Castillo Rico , Adanlawo Temitayo , Everitt Jeffery I. , Hodgin Jeffery B. , Watts Tammara L. , Janowczyk Andrew , Mowery Yvonne M. , Barisoni Laura , Lafata Kyle J. 
Published: October 2024

IntroductionImmune dysregulation plays a major role in cancer progression. The quantification of lymphocytic spatial inflammation may enable spatial system biology, improve understanding of therapeutic resistance, and contribute to prognostic imaging biomarkers.MethodsIn this paper, we propose a knowledge-guided deep learning framework to measure the lymphocytic spatial architecture on human H&amp;E tissue, where the fidelity of training labels is maximized through single-cell resolution image registration of H&amp;E to IHC. We demonstrate that such an approach enables pixel-perfect ground-truth labeling of lymphocytes on H&amp;E as measured by IHC. We then experimentally validate our technique in a genetically engineered, immune-compromised Rag2 mouse model, where Rag2 knockout mice lacking mature lymphocytes are used as a negative experimental control. Such experimental validation moves beyond the classical statistical testing of deep learning models and demonstrates feasibility of more rigorous validation strategies that integrate computational science and basic science.ResultsUsing our developed approach, we automatically annotated more than 111,000 human nuclei (45,611 CD3/CD20 positive lymphocytes) on H&amp;E images to develop our model, which achieved an AUC of 0.78 and 0.71 on internal hold-out testing data and external testing on an independent dataset, respectively. As a measure of the global spatial architecture of the lymphocytic microenvironment, the average structural similarity between predicted lymphocytic density maps and ground truth lymphocytic density maps was 0.86 ± 0.06 on testing data. On experimental mouse model validation, we measured a lymphocytic density of 96.5 ± %1% in a Rag2+/- control mouse, compared to an average of 16.2 ± %5% in Rag2-/- immune knockout mice (p&lt;0.0001, ANOVA-test).DiscussionThese results demonstrate that CD3/CD20 positive lymphocytes can be accurately detected and characterized on H&amp;E by deep learning and generalized across species. Collectively, these data suggest that our understanding of complex biological systems may benefit from computationally-derived spatial analysis, as well as integration of computational science and basic science.
Hypofractionated radiotherapy with simultaneous integrated boost for localized prostate cancer patients: effects on immune system and prediction of toxicity
Authors: D’Auria Fiorella , Valvano Luciana , Calice Giovanni , D’Esposito Vittoria , Cabaro Serena , Formisano Pietro , Bianchino Gabriella , Traficante Antonio , Bianculli Antonella , Lazzari Grazia , Statuto Teodora , Rago Luciana 
Published: October 2024

BackgroundThe other side of radiotherapy (RT), in addition to the cytotoxic effect, is the ability to modulate the immune system in terms of activation or suppression, also depending on the dose and fractionation delivered. This immune RT effect can be detected both locally in the irradiated tumor site and in the peripheral blood. The aim of this study was to assess the consequence of pelvic irradiation on peripheral immune cells and cytokine secretions in localized prostate cancer (PC) patients undergoing pelvic irradiation with a simultaneous moderately hypofractionated prostate/prostate bed boost by Volumetric Modulated Arc Therapy (VMAT). Furthermore, we analyzed whether there was a correlation between these peripheral immune parameters and acute and late genitourinary (GU) and gastrointestinal (GI) toxicity.MethodsThirty-eight PC patients were treated with pelvis irradiation (dose per fraction 1.8 Gy) and simultaneous hypofractionated (median dose per fraction: 2.7 Gy) prostate/prostate bed boost. A longitudinal analysis was performed for 12 months on peripheral blood to assess changes in 9 different lymphocyte subpopulations by flow cytometry and 10 circulating cytokines by Multiplex Luminex assay and ELISA.ResultsOur analysis revealed that basal IFN-γ serum values were significantly lower in the definitive (curative intent for patients with prostate) patient group respect to the post-operative one. All the lymphocyte subsets and IFN-α, IFN-β and Il-2 peripheral concentrations displayed significant variations between the different time points considered. The immune cell population that suffers the greatest RT toxicity in the blood was B lymphocyte. We found an interesting correlation between basal TGF-β1 and late GU toxicity. In particular, TGF-β1 concentrations before RT were significantly higher in patients that experienced grade 2-3 of late GU toxicity, respect to grade 0-1. Exploring possible correlations between some clinical/biological findings and radiation planning parameters, we found no statistical significance.ConclusionsOur study analyzed, in the context of hypofractionated radiotherapy in prostate cancer, different parameters of the peripheral immune system. We have highlighted longitudinally the peripheral behavior of the different lymphocyte subpopulations and of a group of 10 cytokines during the first year after RT. One of the analyzed cytokines, such as TGF-β1, has proven to be promising predictive factor of severe late GU toxicity.
Orbital decompression improves visual function and macular blood perfusion status in patients with thyroid-related eye disease
Authors: Wu Bingxuan , Zhu Min , Zhang Feng , Cao Jiamin , Xie Bingyu , Zhu Ziyi , Xiong Wei 
Published: October 2024

BackgroundOrbital decompression surgery is a treatment option for Thyroid-associated Ophthalmopathy (TAO). However, its effects on visual function and macular perfusion status remain unclear. This study examined how orbital decompression surgery affects visual acuity and macular blood flow in TAO patients and the variation between these two factors.MethodsThis study involved cross-sectional and longitudinal analyses of 54 TAO patients, who were categorized into a normal vision group (n = 40) and an impaired vision group (n = 14) based on the presence of visual impairment before surgery (LogMAR BCVA &gt;0.097). Among the participants, 20 underwent orbital decompression surgery (normal vision group: n = 13, impaired vision group: n = 7). BCVA and IOP were assessed at baseline in patients with TAO, and macular retinal blood perfusion indices were measured using OCTA. One month post-operatively, changes in these indices were compared between the two groups, and the correlation between visual acuity and macular perfusion indices was analyzed.ResultsThe LogMar BCVA in the visual impairment group was significantly worse than that in the normal vision group (p &lt; 0.001), while the W-MVD was significantly lower (p = 0.01). In the visual impairment group, post-operative LogMar BCVA improved significantly (p = 0.038), and W-MVD increased significantly (p = 0.008) compared to baseline. However, there were no significant changes in LogMar BCVA, S-MVD, D-MVD, or W-MVD in the normal vision group from preoperative to postoperative assessments. A strong negative correlation was found between the changes in W-MVD and changes in LogMar BCVA in the eyes of the visual impairment group treated with orbital decompression (Pearson correlation coefficient, R = −0.812, p = 0.05).ConclusionThis study found that TAO patients with visual impairment had lower macular blood flow perfusion than those with normal vision. Endoscopic orbital decompression surgery was found to improve both the best-corrected visual acuity and macular blood flow perfusion in TAO patients with visual impairment, and the improvement in visual acuity was correlated with the improvement in macular blood flow perfusion.
Unraveling the key mechanisms of Gastrodia elata continuous cropping obstacles: soil bacteria Massilia, Burkholderia-Caballeronia-Paraburkholderia, and Dyella along with soil metabolites 4-hydroxy-benzenemethanol and N-(2-butyl)-N-octadecyl-, ethyl ester as crucial indicators
Authors: Duan Mingzheng , Yang Chengcui , Bao Liuyuan , Han Duo , He Haiyan , Zhang Yongzhi , Dong Li , Yang Shunqiang 
Published: October 2024

BackgroundTian-ma (Gastrodia elata) is a traditional medicinal herb found in China. It is used in healthy food and to treat various diseases, therefore cultivated extensively in southwest China. However, continuous cropping of this species has led to various obstacles, such as microbial disease and pest infestation, significantly affecting the production and development of valuable medicinal and food resources. As per the growth habit, soil is presumed to be the primary factor contributing to these obstacles, despite the known issues of continuous cropping obstacles in Gastrodia elata, such as microbial disease, there is a lack of comprehensive understanding of the specific soil bacterial communities and metabolites involved in these processes.MethodsWe analyzed soil samples collected during the year of Tian-ma cultivation (0 Year), after the Tian-ma harvest (1 Year), after two years (2 Year), and three years (3 Year) of fallowing post-cultivation using soil 16S rRNA metabarcoding sequencing by illumina platform and metabolomics (GC–MS/MS). Soil sample collected from the uncultivated field was used as the control (CK).ResultsMetabarcoding sequencing showed high bacterial alpha diversity during the cultivation of Tian-ma (0 Year) and the period of deterioration of soil bacterial community. (1 Year), with decreased anaerobic bacterial abundance and increased copiotrophic bacterial abundance. Bacteria associated with sulfur metabolism also showed increased abundance during the year of cropping obstacles. Further metabolomics approach identified 4-hydroxy-benzenemethanol as an indicator of Tian-ma continuous cropping obstacles. Besides, metabolites of the carbohydrate class were found to be the most abundant during the occurrence of continuous cropping obstacles of Gastrodia elata, suggesting that regulation of soil microbial diversity may be a critical factor in addressing these obstacles. Finally, the correlation analysis indicated a positive association between the abundance of some metabolite, e.g., carbamic acid, N-(2-butyl)-N-octadecyl-, ethyl ester detected after Tian-ma cultivation and the abundance of bacteria capable of degrading toxic metabolites, such as Massilia, Burkholderia-Caballeronia-Paraburkholderia, and Dyella.ConclusionThis study has revealed the specific soil bacteria and metabolic factors related to the continuous cropping obstacles of Gastrodia elata. These findings not only deepen our understanding of the continuous cropping issues but also pave the way for developing effective strategies to overcome them.
The golden batch-driven root cause analysis for anomalies in bioreactor fermentation process
Authors: Luo Dennis , He Meiling , Darko Justice , Ly Seymour Fatime , Maturana Francisco 
Published: October 2024

Bioreactors are essential for the production of biopharmaceuticals and bioproducts, requiring continuous monitoring to ensure quality assurance. Manual processes in manufacturing plants often lead to anomalies such as out-of-trend and out-of-spec incidents, necessitating extensive root cause analysis that typically takes 2–8 weeks. This paper introduces an innovative methodology that uses the golden batch profile as a benchmark to identify deviations and root causes in subsequent industrial batches. The methodology involves normalizing the data and calculating the variances of a specified batch from the golden batch profile. By examining the contribution of each critical process parameter to these variances, the study highlights their importance in root cause analysis. The application of this methodology to the IndPenSim dataset demonstrated its effectiveness by significantly reducing false positives and negatives compared to traditional PCA-based methods. Emphasis on the deviations of critical quality attributes and critical process parameters from the specified batch compared to the golden batch profile offers valuable insights into industrial process analysis. This approach not only enhances anomaly detection accuracy but also improves the efficiency and reliability of biopharmaceutical and bioproduct manufacturing processes.
Neuronal Intranuclear Inclusion Disease Presenting with Acute-Onset Dementia and Cortical Edema: A Case Report
Authors: Feng Xiao , Li Yue , Zhao Qin , Xu Shabei 
Published: October 2024

BackgroundNeuronal Intranuclear Inclusion Disease (NIID) is a neurodegenerative disorder characterized by the formation of eosinophilic inclusions in the neurons, visceral and skin cells. The cause is associated with the GGC nucleotide repeat expansion in the NOTCH2NLC gene. The imaging hallmark of NIID is hyperintensities on diffusion-weighted imaging (DWI) at the corticomedullary junction. Clinical manifestations of NIID are highly heterogeneous. Here, we report a case of NIID presenting with acute-onset dementia and cortical edema.Case presentationWe describe an elderly male patient who presented with sudden dementia within a day. Considering the abrupt onset and the stroke history, we initially diagnosed vascular disease. However, further imaging revealed cortical edema in the temporo-parieto-occipital lobes. Blood and cerebrospinal fluid tests ruled out immunological, metabolic, infectious, or neoplastic etiologies. Genetic testing ultimately confirmed the diagnosis of NIID. Intravenous immunoglobulin (IVIG) therapy did not improve the patient’s symptoms; However, about 1 month after treatment, spontaneous improvement was observed. It is noteworthy that 22 months before the onset of cognitive impairment, the patient’s MRI for headaches already exhibited the typical imaging lesions of this disease in the cerebellum paravermal region.ConclusionPatients with encephalopathy syndrome exhibiting imaging features resembling mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome or Creutzfeldt-Jakob disease should consider the NIID as differential diagnosis. Chronic headaches and symmetric lesions in the cerebellar paravermal region on MRI may be noteworthy indicators of NIID during non-episodic phases.