bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Combined Therapy, Percutaneous Concentrated Autologous Bone Marrow Transplantation, and Low-Intensity Pulsed Ultrasound (LIPUS) in Treatment of Femoral Head Osteonecrosis
Authors: Sugaya Hisashi , Yoshioka Tomokazu , Aoto Katsuya 
Published: June 2018

Objective:
We describe a novel, combined approach to the treatment of osteonecrosis of the femoral head (ONFH) based on concentrated autologous bone marrow aspirates (CABMAT) and low-intensity pulsed ultrasound (LIPUS).

Patients and Methods:
The 7 male and 7 female patients (mean age: 40 years; 22 hips) underwent CABMAT followed by 6 months of continuous LIPUS. The mean follow-up period was 26 months. All patients were assessed using the visual analogue scale (VAS) for pain and the Japanese Orthopedic Association (JOA) hip score. Clinical and plain radiographic evaluations were performed before transplantation and at the most recent follow-up. Computed tomography (CT) scans were obtained before and 12 months after grafting.

Results:
The VAS and JOA scores improved in all patients. Collapse progressed in 8 of the 22 hips, but none required total hip arthroplasty. The mean volume of new bone formation 12 months' post-transplantation as seen on CT was 1256 mm3. New bone formation was observed in all patients.

Discussion:
The multiple benefits of LIPUS include improved local blood flow, accelerated production of angiogenesis-inducing cytokines, accelerated differentiation of mesenchymal stem cells to form osteoblasts, and the inhibition of osteoclast production and differentiation. This combined therapy is noninvasive, feasible, and joint-preserving in most ONFH patients.
The Present Situation of the Low-Intensity Pulsed Ultrasound Treatments According to the Japanese National Open Data
Authors: Jingushi Seiya 
Published: June 2018

Objective:
The change of the current Japanese population structure due to an aging society with a low birthrate influences the status of fracture patients. This may cause the change about the usage of the low-intensity pulsed ultrasound (LIPUS) treatments to accelerate a fracture repair. The present situation was examined using the Japanese National open big data.

Materials and Methods:
The first open data since April 2015 till March 2016 was used from the homepage of the Ministry of Health, Labour and Welfare. Among the data about K-code of the various operations, the total number of K044, K045, K046, K046-2, K073 and K073-2 was used as the number of fracture treatments. The number of the LIPUS treatments was counted as K047-2 and K047-3.

Results:
LIPUS treatment was used most in the patients more than 20 year old. The ratio of the LIPUS treatments per the fracture treatments was approximately 6% in the male patients more than 20 year old. In the female patients more than 20 year old, the older they were, the higher the ratio was. The rate at more than 90 year old was over 7%.

Discussion:
The current change of the population structure may cause an increase of the necessity of LIPUS treatment for the fracture patients.
Differences in Morbidity and Mortality Rates in Black, White, and Hispanic Very Preterm Infants Among New York City Hospitals
Authors: Howell Elizabeth A. , Janevic Teresa , Hebert Paul L. , Egorova Natalia N. , Balbierz Amy , Zeitlin Jennifer 
Published: June 2018

(Abstracted from JAMA Pediatr 2018;172:269–277)
Currently, 98% of neonatal intensive care units (NICUs) have a risk-adjusted rate of mortality equal to the best 10% of units in 2005, a statistic showcasing the significant improvements in neonatal care over the past decade. Despite improvements, research shows significant racial disparities in neonatal mortality and morbidity, with non-Hispanic black neonates and Hispanic neonates experiencing a disproportionate amount of these adverse events.
Congenital Heart Defects in IVF/ICSI Pregnancy: Systematic Review and Meta-analysis
Authors: Giorgione V. , Parazzini F. , Fesslova V. , Cipriani S. , Candiani M. , Inversetti A. , Sigismondi C. , Tiberio F. , Cavoretto P. 
Published: June 2018

(Abstracted from Ultrasound Obstet Gynecol 2018;51:33–42)
Despite congenital heart disease (CHD) being the most common type of congenital defect, in the majority of cases, the etiology remains unclear. Previous studies analyzing the risk of CHD after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) show a slightly elevated risk when compared with spontaneously conceived infants.
Ovarian Cancer Screening With the Risk Of Ovarian Cancer Algorithm (ROCA): Good, Bad, or Just Expensive?
Authors: Naumann R. Wendel , Brown Jubilee 
Published: June 2018

(Abstracted from Gynecol Oncol 2018;149:117–120)
Although treatment of early-stage ovarian cancer has an excellent prognosis, the majority of women present with advanced disease. A minority of patients present with stage I disease and screening of these patients could potentially increase detection or detect patients with advanced disease at a point where surgery might be more effective.
Cancer Risk After Midurethral Sling Surgery Using Polypropylene Mesh
Authors: Altman Daniel , Rogers Rebecca G. , Yin Li , Tamussino Karl , Ye Weimin , Iglesia Cheryl B. 
Published: June 2018

(Abstracted from Obstet Gynecol 2018;131:469–474)
The criterion standard surgery for stress urinary incontinence (SUI) is the midurethral sling procedure. Since its introduction in 1997, this procedure has been widely used for pelvic reconstructive surgery.
Methicillin-Resistant Staphylococcus aureus Parotitis Leading to Mortality in an Adolescent Male
Authors: Basinger Janet M. , Fiester Steven E. , Fulcher James W. 
Published: June 2018

Abstract
A case of toxic shock syndrome associated with methicillin-resistant Staphylococcus aureus parotitis in a 13-year-old male is presented. He was initially diagnosed with left-sided parotitis by his primary care physician, was started on sulfamethoxazole/trimethoprim, and became severely ill the following day. He was transported to the hospital after a syncopal episode at home and was found to have altered mental status, hypotension, and hypoxia. He was transferred to a larger care facility and died en route despite aggressive resuscitation. At autopsy, he was found to have a severe left-sided parotitis, severe pulmonary congestion, edema, and pneumonia, as well as bilateral lower limb hemorrhagic lesions. Blood cultures from the time of admission and at autopsy grew methicillin-resistant Staphylococcus aureus, which is rarely reported as the sole cause of parotitis. In addition, although S. aureus bacteremia is not necessarily a rare complication of a parotid gland infection, it is exceedingly rare in an immunocompetent adolescent.
Andreev reflection spectroscopy in transition metal oxides
Authors: Yates Karen A. , Cohen Lesley F. 
Published: June 2018

Here we review the literature concerning measurement of the Andreev reflection between a superconductor (S) and ferromagnet (F), with particular attention to the case where the ferromagnet is a transition metal oxide. We discuss the practicality of utilization of the current models for determination of the transport current spin polarization and examine the evidence for Andreev bound states.
This article is part of the theme issue ‘Andreev bound states’.
Genome-wide CNV study and functional evaluation identified CTDSPL as tumour suppressor gene for cervical cancer
Authors: Chen Dan , Wang Shuai , Li Zhenli , Cui Tao , Chen Yao , Song Junjiao , Magnusson Patrik KE , Wang Huibo , Zhang Dandan , Gyllensten Ulf 
Published: June 2018

AbstractWe have investigated copy number variations (CNVs) in relation to cervical cancer by analyzing 731,422 single-nucleotide polymorphisms (SNPs) in 1,034 cervical cancer cases and 3,948 controls, followed by replication in 1,396 cases and 1,057 controls. We found that a 6367bp deletion in intron 1 of the CTD small phosphatase like gene (CTDSPL) was associated with 2.54-fold increased risk of cervical cancer (odds ratio =2.54, 95% confidence interval =2.08-3.12, P=2.0×10−19). This CNV is one of the strongest genetic risk variants identified so far for cervical cancer. The deletion removes the binding sites of zinc finger protein 263, binding protein 2 and interferon regulatory factor 1, and hence downregulates the transcription of CTDSPL. HeLa cells expressing CTDSPL showed a significant decrease in colony-forming ability. Compared with control groups, mice injected with HeLa cells expressing CTDSPL exhibited a significant reduction in tumour volume. Furthermore, CTDSPL-depleted immortalized End1/E6E7 could form tumours in NOD-SCID mice.
Predicting bacterial growth conditions from mRNA and protein abundances
Authors: Caglar Mehmet U. , Hockenberry Adam J. , Wilke Claus O. 
Published: June 2018

AbstractCells respond to changing nutrient availability and external stresses by altering the expression of individual genes. Condition-specific gene expression patterns may provide a promising and low-cost route to quantifying the presence of various small molecules, toxins, or species-interactions in natural environments. However, whether gene expression signatures alone can predict individual environmental growth conditions remains an open question. Here, we used machine learning to predict 16 closely-related growth conditions using 155 datasets of E. coli transcript and protein abundances. We show that models are able to discriminate between different environmental features with a relatively high degree of accuracy. We observed a small but significant increase in model accuracy by combining transcriptome and proteome-level data, and we show that stationary phase conditions are typically more difficult to distinguish from one another than conditions under exponential growth. Nevertheless, with sufficient training data, gene expression measurements from a single species are capable of distinguishing between environmental conditions that are separated by a single environmental variable.
Miniscule differences between the sex chromosomes in the giant genome of a salamander,Ambystoma mexicanum
Authors: Keinath Melissa C. , Timoshevskaya Nataliya , Timoshevskiy Vladimir A. , Voss S. Randal , Smith Jeramiah J. 
Published: June 2018

ABSTRACTIn the Mexican axolotl (Ambystoma mexicanum) sex is known to be determined by a single Mendelian factor, yet the sex chromosomes of this model salamander do not exhibit morphological differentiation that is typical of many vertebrate taxa that possess a single sex-determining locus. Differentiated sex chromosomes are thought to evolve rapidly in the context of a Mendelian sex-determining gene and, therefore, undifferentiated chromosomes provide an exceptional opportunity to reconstruct early events in sex chromosome evolution. Whole chromosome sequencing, whole genome resequencing (48 individuals from a backcross of axolotl and tiger salamander) andin situhybridization were used to identify a homomorphic chromosome that carries anA. mexicanumsex determining factor and identify sequences that are present only on the W chromosome. Altogether, these sequences cover ~300 kb, or roughly 1/100,000thof the ~32 Gb genome. Notably, these W-specific sequences also contain a recently duplicated copy of the ATRX gene: a known component of mammalian sex-determining pathways. This gene (designatedATRW) is one of the few functional (non-repetitive) genes in the chromosomal segment and maps to the tip of chromosome 9 near the markerE24C3, which was previously found to be linked to the sex-determining locus. These analyses provide highly predictive markers for diagnosing sex inA. mexicanumand identifyATRWas a strong candidate for the primary sex determining locus or alternately a strong candidate for a recently acquired, sexually antagonistic gene.AUTHOR SUMMARYSex chromosomes are thought to follow fairly stereotypical evolutionary trajectories that result in differentiation of sex-specific chromosomes. In the salamander A. mexicanum (the axolotl), sex is determined by a single Mendelian locus, yet the sex chromosomes are essentially undifferentiated, suggesting that these sex chromosomes have recently acquired a sex locus and are in the early stages of differentiating. Although Mendelian sex determination was first reported for the axolotl more than 70 years ago, no sex-specific sequences have been identified for this important model species. Here, we apply new technologies and approaches to identify and validate a tiny region of female-specific DNA within the gigantic genome of the axolotl (1/100,000th of the genome). This region contains a limited number of genes, including a duplicate copy of the ATRX gene which, has been previously shown to contribute to mammalian sex determination. Our analyses suggest that this gene, which we refer to as ATRW, evolved from a recent duplication and presents a strong candidate for the primary sex determining factor of the axolotl, or alternately a recently evolved sexually antagonistic gene.
Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors
Authors: Nishino Koichiro , Arai Yoshikazu , Takasawa Ken , Toyoda Masashi , Yamazaki-Inoue Mayu , Sugawara Tohru , Akutsu Hidenori , Nishimura Ken , Ohtaka Manami , Nakanishi Mahito , Umezawa Akihiro 
Published: June 2018

AbstractHuman induced pluripotent stem cells (iPSCs) are established by introducing several reprogramming factors, such as OCT3/4, SOX2, KLF4, c-MYC. Because of their pluripotency and immortality, iPSCs are considered to be a powerful tool for regenerative medicine. To date, iPSCs have been established all over the world by various gene delivery methods. All methods induced high-quality iPSCs, but epigenetic analysis of abnormalities derived from differences in the gene delivery methods has not yet been performed. Here, we generated genetically matched human iPSCs from menstrual blood cells by using three kinds of vectors, i.e., retrovirus, Sendai virus, and episomal vectors, and compared genome-wide DNA methylation profiles among them. Although comparison of aberrant methylation revealed that iPSCs generated by Sendai virus vector have lowest number of aberrant methylation sites among the three vectors, the iPSCs generated by non-integrating methods did not show vector-specific aberrant methylation. However, the differences between the iPSC lines were determined to be the number of random aberrant hyper-methylated regions compared with embryonic stem cells. These random aberrant hyper-methylations might be a cause of the differences in the properties of each of the iPSC lines.
Assessment of Metagenomic MinION and Illumina sequencing as an approach for the recovery of whole genome sequences of chikungunya and dengue viruses directly from clinical samples
Authors: Kafetzopoulou Liana E. , Efthymiadis Kyriakos , Lewandowski Kuiama , Crook Ant , Carter Dan , Osborne Jane , Aarons Emma , Hewson Roger , Hiscox Julian A. , Carroll Miles W. , Vipond Richard , Pullan Steven T. 
Published: June 2018

AbstractThe recent global emergence and re-emergence of arboviruses has caused significant human disease. Common vectors, symptoms and geographical distribution make differential diagnosis both important and challenging. We performed metagenomic sequencing using both the Illumina MiSeq and the portable Oxford Nanopore MinION to study the feasibility of whole genome sequencing from clinical samples containing chikungunya or dengue virus, two of the most important arboviruses. Direct metagenomic sequencing of nucleic acid extracts from serum and plasma without viral enrichment allowed for virus and coinfection identification, subtype determination and in the majority of cases elucidated complete or near-complete genomes adequate for phylogenetic analysis. This work demonstrates that metagenomic whole genome sequencing is feasible for over 90% and 80% of chikungunya and dengue virus PCR-positive patient samples respectively. It confirms the feasibility of field metagenomic sequencing for these and likely other RNA viruses, highlighting the applicability of this approach to front-line public health.
Lysophosphatidic acid provokes fibroblast chemotaxis through combinatorial regulation of myosin II
Authors: Asokan Sreeja B. , Johnson Heath E. , Sondek John , Shutova Maria S. , Svitkina Tatyana M. , Haugh Jason M. , Bear James E. 
Published: June 2018

SUMMARYLysophophatidic acid (LPA), a biologically active phospholipid that is ubiquitously present in tissues and organs, provokes cellular responses such as proliferation, apoptosis, differentiation and migration via activation of G-protein coupled receptors. These receptors activate a broad range of intracellular signaling cascades to mediate these responses. Using microfluidic chambers that generate and maintain stable gradients, we observed that chemotaxis of fibroblasts to LPA has higher directional fidelity than chemotaxis provoked by the receptor tyrosine kinase (RTK) ligand platelet-derived growth factor (PDGF). Unlike fast moving amoeboid cells, mesenchymal cells such as fibroblasts do not require PI3K for chemotaxis to a GPCR ligand. In addition, the Arp2/3 complex is not required for fibroblast GPCR-based chemotaxis in either 2D or 3D environments. Our data indicate that combinatorial regulation of myosin II involving global activation by RhoA/ROCK and local inhibition of myosin II at the leading edge by PKC results in highly efficient chemotaxis of fibroblasts to LPA. Based on these observations, we develop a simple mathematical model to explain how dual regulation of myosin II is responsible for enhanced chemotaxis in LPA gradients relative to PDGF. Using pharmacological approaches, we test predictions of this model and modulate the fidelity of LPA and PDGF chemotaxis.
An Optically Decodable Bead Array for Linking Imaging and Sequencing with Single-Cell Resolution
Authors: Yuan Jinzhou , Sheng Jenny , Sims Peter A. 
Published: June 2018

AbstractOptically decodable beads link the identity of an analyte or sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and flow-based expression profiling technologies. We have combined microfluidics with optically decodable beads to link phenotypic analysis of living cells to sequencing. As a proof-of-concept, we applied this to demonstrate an accurate and scalable tool for connecting live cell imaging to single-cell RNA-Seq called Single Cell Optical Phenotyping and Expression (SCOPE-Seq).
Ambidextrous organization and agility in big data era
Authors: Rialti Riccardo , Marzi Giacomo , Silic Mario , Ciappei Cristiano 
Published: June 2018

PurposeThe purpose of this paper is to explore the effect of big data analytics-capable business process management systems (BDA-capable BPMS) on ambidextrous organizations’ agility. In particular, how the functionalities of BDA-capable BPMS may improve organizational dynamism and reactiveness to challenges of Big Data era will be explored.Design/methodology/approachA theoretical analysis of the potential of BDA-capable BPMS in increasing organizational agility, with particular attention to the ambidextrous organizations, has been performed. A conceptual framework was subsequently developed. Next, the proposed conceptual framework was applied in a real-world context.FindingsThe research proposes a framework highlighting the importance of BDA-capable BPMS in increasing ambidextrous organizations’ agility. Moreover, the authors apply the framework to the cases of consumer-goods companies that have included BDA in their processes management.Research limitations/implicationsThe principal limitations are linked to the need to validate quantitatively the proposed framework.Practical implicationsThe value of the proposed framework is related to its potential in helping managers to fully understand and exploit the potentiality of BDA-capable BPMS. Moreover, the implications show some guidelines to ease the implementation of such systems within ambidextrous organizations.Originality/valueThe research offers a model to interpret the effects of BDA-capable BPMS on ambidextrous organizations’ agility. In this way, the research addresses a significant gap by exploring the importance of information systems for ambidextrous organizations’ agility.
Firm-specific corporate governance and analysts’ earnings forecast characteristics
Authors: Yu Minna , Wang Yanming 
Published: June 2018

Purpose
The purpose of this paper is to examine the impact of corporate governance on the capital market participants’ abilities to forecast future performance, as measured by the properties of analysts’ earnings forecasts in Asian stock markets.

Design/methodology/approach
This paper hypothesizes that higher corporate governance is associated with lower forecast errors, lower forecast dispersion and lower forecast revision volatility.

Findings
These predictions are supported with a sample of companies across eleven Asian economies over 2004-2012. The results of this paper suggest that corporate governance plays a significant role in the predictability of firm’s future performance and, therefore, improves the financial environment in Asian stock markets. Furthermore, the impact of corporate governance on analysts’ forecast properties is more pronounced in countries with strong investor protection.

Research/limitations/implications
The authors acknowledge the following limitations of this paper. First, the results of this paper may be subject to omitted-variable bias and endogeneity issue. The authors have used control variables in the regressions to reduce the omitted variable bias. The authors have run lead-lag regressions to address causality issue. Second, CLSA corporate governance scores are collected for largest companies in each jurisdiction. Therefore, the sample is biased towards the largest companies in those jurisdictions and may not be representative of the average firm in the Asia.

Originality/value
The results of this paper speak to the benefit of having strong corporate governance in terms of reducing the information asymmetry between investors and corporate management.
The effect of managerial and institutional ownership on corporate social responsibility disclosure
Authors: Nurleni Nurleni , Bandang Agus , Darmawati Darmawati , Amiruddin Amiruddin 
Published: June 2018

Purpose
This study aims to analyze the effect of ownership structure that consists of managerial ownership and institutional ownership of the extensive of corporate social responsibility (CSR) disclosure.

Design/methodology/approach
The population in this study is manufacturing companies listed in Indonesia Stock Exchange (BEI), as the manufacturing companies are considered to have great potential on environmental damage (Mathews, 2000). The selected sample were the companies which meet certain criteria (purposive sampling) which published the complete annual financial statements from 2011 to 2015. This study used an analysis method using partial least square (WarpPLS) to assess the effect of the structure of ownership consists of managerial ownership and institutional ownership on the extent of the CSR disclosure.

Findings
The results showed that there is a direct effect of a negative and significant correlation between managerial ownership on CSR disclosure, and there is a direct effect of a positive and significant correlation between institutional ownership on CSR disclosure.

Originality/value
Originality of this paper shows PLS (WarpPLS) that applied to determine the effect between variables managerial and institutional ownership on CSR disclosure. This research is collected data financial statements and annual reports of manufacturing companies obtained from the Indonesia Capital Market Reference Center (PRPM), which is located in the Indonesia Stock Exchange (IDX), which there has not been research by the methods and the same location.