bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel High‐Throughput Immunoaffinity LC–MS/MS Assay for P‐III‐NP and Other Fragments of Type III Procollagen in Human Serum
Authors: Huynh Huu‐Hien , Barahona‐Carrillo Lili , Moncrieffe Danielle , Cowan David A. , Forrest Katrina , Becker Jessica O. , Emrick Michelle A. , Thomas Andreas , Thevis Mario , Eichner Daniel , Byers Peter H. , Miller Geoffrey D. , Hoofnagle Andrew N. 
Published: October 2024

ABSTRACTThe amino‐terminal propeptide of type III procollagen (P‐III‐NP) is used with IGF‐I to detect the illicit use of growth hormone and to monitor growth hormone therapy. However, the only currently available assays for P‐III‐NP are immunoassays, which are not well harmonized. In addition, other fragments of type III procollagen may better evaluate collagen turnover. We aimed to develop a high‐throughput assay using immunoaffinity enrichment coupled to ultra‐high‐performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) to quantify peptides belonging to three different regions of type III procollagen in human serum simultaneously. To facilitate higher throughput, we transferred the assay from microcentrifuge tubes to a 96‐well plate format with partially automated pipetting. The method was linear (Pearson's R ≥ 0.994) over an estimated concentration range of 1.35–13.3 nM, 0.04–2.28 nM, and 0.26–5.1 nM for each surrogate peptide of P‐III‐NP, collagen degradation products, and the carboxyl‐terminal propeptide, respectively. Intra‐day and inter‐day imprecision were both < 13.6%, and the results of robustness testing were also encouraging. The method was successfully applied to capillary blood samples obtained using Tasso+ microsampling devices. Modest correlation of P‐III‐NP concentration was observed between our new method and a WADA‐approved immunoassay (N = 40, Pearson's R = 0.789) with a significant bias of −87.8%. Our method simultaneously quantifies four peptides belonging to three regions of type III procollagen in human serum. High bias between assays highlights the need for common higher‐order calibrators or reference materials to help improve the comparability of results across laboratories.
Proteomic biomarkers and pathway analysis for progression to heart failure in three epidemiological representative cohorts
Authors: Dieden Anna , Girerd Nicolas , Ottosson Filip , Molvin John , Pareek Manan , Melander Olle , Bachus Erasmus , Råstam Lennart , Lindblad Ulf , Daka Bledar , Leósdóttir Margrét , Nilsson Peter M. , Olsen Michael H. , Clark Andrew L. , Cleland John G.F. , Delles Christian , González Arantxa , Lamiral Zohra , Duarte Kevin , Rossignol Patrick , Zannad Faiez , Gudmundsson Petri , Jujić Amra , Magnusson Martin 
Published: October 2024

ABSTRACTAimsBiomarkers associated with asymptomatic ventricular dysfunction might improve risk stratification and identify pathways leading to heart failure (HF). We explored the association between proteomic biomarkers and left ventricular hypertrophy (LVH), diastolic dysfunction (DD) and incident HF in three population‐based cohorts.Methods and resultsA chip was used to measure 92 protein biomarkers in blood samples from >1500 Malmö Preventive Project (MPP) participants, of whom 514 had LVH (34%), 462 had DD (32.4%) and, over a median follow‐up of 13 (11–14) years, 130 developed HF (7.7%). Findings were confirmed in the STANISLAS (n > 1500, 238 participants with LVH, 76 with DD) and HOMAGE case‐control (562 cases of incident HF, 871 controls) cohorts. In multivariable logistic or Cox regression analyses adjusted for age, sex and cardiovascular risk factors, N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) was associated with LVH, DD and incident HF in all cohorts: MPP (LVH odds ratio [OR] [95% confidence interval] 1.48 [1.28–1.71]; DD OR 1.71 [1.53–1.92]; HF HR 1.98 [1.66–2.36]); STANISLAS (LVH OR 1.20 [1.02–1.41]; DD OR 1.46 [1.12–1.90]); HOMAGE (HF HR 1.85 [1.62–2.12]). Galectin‐4, growth differentiation factor 15 and suppression of tumorigenicity‐2 were associated with incident HF in MPP and HOMAGE. A pathway enrichment analysis suggested that inflammation and viral infection were related to incident HF.ConclusionIn conclusion, our study reinforces the role of NT‐proBNP as a key biomarker for asymptomatic cardiac dysfunction and incident HF, consistent with its established use in clinical practice. This underscores the value of NT‐proBNP for identifying patients at high risk for HF, and provides insights into pathways leading to HF and potential therapeutic targets.
A Bibliometric and Knowledge‐Map Analysis of Macrophage Polarization in Insulin Resistance From 1999 to 2023
Authors: Lin Chuning , Chen Yuan , Ge Yao , Niu Huimin , Zhang Xinyi , Jiang Feng , Wu Chuyan 
Published: October 2024

ABSTRACTBackgroundDespite numerous studies confirming the association between insulin resistance (IR) and macrophage polarization, there is a lack of bibliometric analysis in this area. Therefore, our objective is to conduct a comprehensive analysis of published literature and identify potential future research trends using bibliometrics.MethodPublications on the topic of macrophage polarization in IR were gathered from the Web of Science Core Collection database (WoSCC) spanning the years 1999–2023. Bibliometric analysis and visualization were conducted using VOSviewers, CiteSpace, the R package “bibliometrix” and Tableau Public.ResultA total of 3435 articles published between 1999 and 2023 were included in the analysis. These articles originated from 75 countries, with the United States and China leading in contributions. The top five research institutions are the University of California, San Diego, Harvard University, the University of Michigan, Shanghai Jiao Tong University, and Huazhong University of Science and Technology. In this research domain, Diabetes is the most frequently published journal, and the Journal of Clinical Investigation is the most co‐cited. Among the 19,398 authors contributing to these publications, Lumeng CN. not only authored the most papers but also received the highest number of co‐citations. “Insulin resistance” emerges as a primary keyword in the analysis of emerging research hotspots.ConclusionFor the first time, bibliometric methods have been employed to conduct a comprehensive summary of papers relevant to macrophage polarization in IR. This study aims to identify the current research direction and future research hotspots, offering valuable guidance and insights for scholars in the field.
Retinoic Acid (RA): A Critical Immunoregulatory Molecule in Asthma and Allergies
Authors: Lotfi Ramin 
Published: October 2024

ABSTRACTIntroductionAsthma and allergies are chronic inflammatory disorders that are triggered owing to aberrant responses of the immune system against typically innocent environmental substances. Retinoic acid (RA) represents a biologically active metabolite of vitamin A (VA) and high‐affinity ligand for RA receptor (RAR) that is implicated in a wide variety of biological processes, including cell proliferation, differentiation, apoptosis, organogenesis, reproduction, and immune responses. In the immune system, RA contributes to the induction of regulatory T (Treg) cells, adhesion molecules required for homing of B and T cells in the gut, and tolerance. Noteworthy, RA has a pivotal role in maintaining the balance of Th17‐Treg cells and is also indispensable for appropriate responses of T helper (Th) cells.AimsThis mini‐review article intends to expose the immune functions of RA, with an emphasis on the enzymatic pathways converting VA into RA and its receptor‐dependent actions in asthma and allergies.ConclusionsRecent findings have depicted that RA levels are reduced in asthma and allergies and that treatment with RA alleviates allergy symptoms and airway inflammation. RA also modulates allergic airway disorders by inhibiting Th2/Th17 response and increasing Treg cells. Therefore, RA could be considered a novel and promising therapeutic agent to be studied and used for treating these diseases.
Examining self‐efficacy among recent graduates of postgraduate dental education programs
Authors: Irwin Scott P. , Durning Steven J. , Dong Ting 
Published: October 2024

AbstractPurposeTo examine the perceived self‐efficacy of dentists who recently completed Graduate Dental Education (GDE) programs and identify how closely it aligns with their supervisors’ assessments of them. Self‐efficacy has been associated with academic pursuits, motivation, and engagement, which may affect how dental providers practice, seek continuing education, and pursue future opportunities. MethodsRecent graduates of military GDE programs rated their self‐efficacy on specific tasks within each of the seven domains of dental competencies. Their supervisors completed a similar survey, rating the graduate's performance in the same tasks. Graduates’ mean ratings were calculated for each domain, spearman correlations were calculated for all graduate‐supervisor task ratings, and the magnitude of differences between graduate and supervisor domain means were examined.ResultsGraduates’ perceived self‐efficacy ranged from 3.57 to 4.41 out of 5.0. Correlations for each task were universally weak (ρ  =  ‐0.04–0.27). Correlations for domain means were also weak (ρ  =  0.06–0.14). Overall, graduates rated themselves lower than their supervisors, with mean differences ranging from ‐0.17 (p = 0.003, Cohen's d = 0.20) for Professionalism to ‐0.95 (p < 0.001, Cohen's d = 0.90) for Health Promotion.ConclusionsOverall, graduates’ perceived self‐efficacy was moderate to high for 26 tasks across seven domains. However, in aggregate, graduates underestimated their abilities compared to performance measures from their current supervisors, although effect sizes were small. The accuracy of graduates’ self‐efficacy varied by program length and the clinical specialty of their supervisors. High‐performing graduates always underestimated themselves while low‐performing graduates often overestimated themselves.
The role of regulatory focus types in the improvement of perinatal oral health knowledge among dental residents
Authors: Kar Kumari Saswati , Cui Shasha , Sanders Michelle , Meng Yuntian , Jallad Nisreen Al , Wu TongTong , Cacciato Rita , Xiao Jin 
Published: October 2024

AbstractObjectivesThis study aims to assess dental residents’ perinatal oral health (POH) knowledge and Regulatory Focus Types' (RFT) impact on their knowledge before and after education.Materials and methodsA total of 53 dental residents from upstate New York participated from 2019 to 2021. A validated questionnaire collected demographic data, resident's POH knowledge, RFT, and self‐reported burnout levels. In the wave 1 cross‐sectional setting, 22 residents participated; while in the wave 2 longitudinal setting, 31 residents completed the questionnaire at the baseline and an 1‐hour POH lecture. Multiple linear regression assesses factors related to baseline and post‐education POH knowledge and burnout, and logistic regression examined factors related to the RFT types (high‐on‐promotion and high‐on‐prevention).ResultsOverall, 43.40% of the dental residents were high‐on‐promotion focus, and 47.17% were high‐on‐prevention focus. Baseline POH knowledge was relatively low, with an average score of 2.57 ± 1.05 out of the maximum score of 5. Residents with high promotion‐focus had statistically significant lower scores on the baseline POH (p < 0.05), while high prevention‐focused residents demonstrated less improvement of POH following perinatal oral health education (p < 0.05). Residents who were high on‐promotion appeared to have a lower burnout rate (p < 0.001).ConclusionsThe study revealed that education effectively enhanced POH knowledge among participating dental residents. RFT significantly influenced knowledge improvement, with high promotion‐focused residents showing more substantial gains. To encourage adherence to perinatal oral health policies and guidelines among dental practitioners, future educational approach should consider practitioners’ RFT to improve knowledge adoption.
Cross‐cultural adaptation and validation of a French version of the perceived personal control questionnaire as an outcome measure instrument for genetic counseling
Authors: Raîche Camille , Lapointe Julie , Villafane‐Bernier Célia , Chiquette Jocelyne , Bouchard Karine , Pelletier Sylvie , Omeranovic Arian , Fortier Philippe , Brousseau Claire , Lauzier Sophie , Hébert Johanne , Dorval Michel , Nabi Hermann 
Published: October 2024

AbstractThe perceived personal control (PPC) questionnaire serves as an instrument to assess the concept of PPC, which refers to a person's perception of their ability to achieve positive outcomes while avoiding the negative effects of a given situation. Developed and used as a patient‐reported outcome measure (PROM) in genetic counseling, the PPC questionnaire has been translated and validated in several languages, but not in French. The aim of this study was to cross‐culturally adapt and validate a French version of the PPC questionnaire to evaluate genetic counseling services for hereditary breast and ovarian cancer (HBOC). After the translation into French, cognitive interviews were conducted with nine participants who had attended genetic counseling for HBOC to examine the adequacy of this French version and to verify participants' understanding of the questionnaire items. Cognitive interview data suggested that slight modifications should be made to four of the nine items and that it would be beneficial to add a short introduction to ensure that participants' interpretation corresponded to the intended meaning. Psychometric validation was then conducted with 99 participants who had also attended genetic counseling for HBOC. Counselees completed the questionnaire before and after their genetic consultation. The acceptability of the questionnaire was demonstrated by the presence of few missing items. The original three‐factor solution was not confirmed by our exploratory factor analysis, suggesting that the questionnaire should be used as a one‐dimensional instrument. The internal consistency of the questionnaire was high, with Cronbach's alpha of 0.89 before genetic counseling and 0.88 after. The significant increase in PPC scores before and after genetic counseling supports the responsiveness of the questionnaire. Convergent validity was confirmed by positive association with counselees' satisfaction with genetic counseling. These properties suggest the French PPC questionnaire is a valuable instrument for use as a PROM in genetic counseling research.
Preferences of parents from diverse backgrounds on genomic screening of apparently healthy newborns
Authors: Gold Nina B. , Omorodion Jacklyn O. , del Rosario Maya C. , Rivera‐Cruz Greysha , Hsu Celeste Y. , Ziniel Sonja I. , Holm Ingrid A. 
Published: October 2024

AbstractGenomic sequencing has been proposed as a strategy to expand newborn screening. Perspectives on genomic newborn screening from parents of diverse racial, ethnic, and socioeconomic backgrounds are needed to shape equitable implementation of this modality. We conducted 20 semi‐structured interviews (15 English, 5 Spanish) and seven focus groups (4 English, 3 Spanish) with parents from diverse backgrounds to assess their perspectives regarding which disorders and variants might be screened, data privacy, and barriers to pursuing specialized care. Parents felt that genomic newborn screening would provide them with improved understanding of their children's health and had the potential to yield health and personal benefits. Themes that became evident included: interest in childhood and family health risks, the value of emotional preparation and personal planning, understanding of uncertain and low‐risk results, concerns regarding data privacy, and concerns about support following the receipt of a positive newborn screening result. The expected benefits and concerns expressed by parents of diverse backgrounds regarding genomic newborn screening should guide future policy decisions. Their preferences should be considered prior to the implementation of large‐scale genomic newborn screening programs.
3D Vortex‐Energetics in the Left Pulmonary Artery for Differentiating Pulmonary Arterial Hypertension and Pulmonary Venous Hypertension Groups Using 4D Flow MRI
Authors: Elbaz Mohammed S.M. , Shafeghat Melika , Freed Benjamin H. , Sarnari Roberto , Zilber Zachary , Avery Ryan , Markl Michael , Allen Bradley D. , Carr James 
Published: October 2024

BackgroundPulmonary hypertension (PH) is a life‐threatening. Differentiation pulmonary arterial hypertension (PAH) from pulmonary venous hypertension (PVH) is important due to distinct treatment protocols. Invasive right heart catheterization (RHC) remains the reference standard but noninvasive alternatives are needed.Purpose/HypothesisTo evaluate 4D Flow MRI‐derived 3D vortex energetics in the left pulmonary artery (LPA) for distinguishing PAH from PVH.Study TypeProspective case–control.Population/SubjectsFourteen PAH patients (11 female) and 18 PVH patients (9 female) diagnosed from RHC, 23 healthy controls (9 female).Field Strength/Sequence1.5 T; gradient recalled echo 4D flow and balanced steady‐state free precession (bSSFP) cardiac cine sequences.AssessmentLPA 3D vortex cores were identified using the lambda2 method. Peak vortex‐contained kinetic energy (vortex‐KE) and viscous energy loss (vortex‐EL) were computed from 4D flow MRI. Left and right ventricular (LV, RV) stroke volume (LVSV, RVSV) and ejection fraction (LVEF, RVEF) were computed from bSSFP. In PH patients, mean pulmonary artery pressure (mPAP), pulmonary capillary wedge pressure (PCWR) and pulmonary vascular resistance (PVR) were determined from RHC.Statistical TestsMann–Whitney U test for group comparisons, Spearman's rho for correlations, logistic regression for identifying predictors of PAH vs. PVH and develop models, area under the receiver operating characteristic curve (AUC) for model performance. Significance was set at P < 0.05.ResultsPAH patients showed significantly lower vortex‐KE (37.14 [14.68–78.52] vs. 76.48 [51.07–120.51]) and vortex‐EL (9.93 [5.69–25.70] vs. 24.22 [12.20–32.01]) than PVH patients. The combined vortex‐KE and LVEF model achieved an AUC of 0.89 for differentiating PAH from PVH. Vortex‐EL showed significant negative correlations with mPAP (rho = −0.43), PCWP (rho = 0.37), PVR (rho = −0.64). In the PAH group, PVR was significantly negatively correlated with LPA vortex‐KE (rho = −0.73) and vortex‐EL (rho = −0.71), and vortex‐KE significantly correlated with RVEF (rho = 0.69), RVSV, (rho = 0.70). In the PVH group, vortex‐KE (rho = 0.52), vortex‐EL significantly correlated with RVSV (rho = 0.58).Data ConclusionThese preliminary findings suggest that 4D flow MRI‐derived LPA vortex energetics have potential to noninvasively differentiate PAH from PVH and correlate with invasive hemodynamic parameters.Evidence Level1Technical EfficacyStage 3
Improving Accuracy and Reproducibility of Cartilage T2 Mapping in the OAI Dataset Through Extended Phase Graph Modeling
Authors: Barbieri Marco , Gatti Anthony A. , Kogan Feliks 
Published: October 2024

BackgroundThe Osteoarthritis Initiative (OAI) collected extensive imaging data, including Multi‐Echo Spin‐Echo (MESE) sequences for measuring knee cartilage T2 relaxation times. Mono‐exponential models are used in the OAI for T2 fitting, which neglects stimulated echoes and B1 inhomogeneities. Extended Phase Graph (EPG) modeling addresses these limitations but has not been applied to the OAI dataset.PurposeTo assess how different fitting methods, including EPG‐based and exponential‐based approaches, affect the accuracy and reproducibility of cartilage T2 in the OAI dataset.Study TypeRetrospective.PopulationFrom OAI dataset, 50 subjects, stratified by osteoarthritis (OA) severity using Kellgren–Lawrence grades (KLG), and 50 subjects without OA diagnosis during OAI duration were selected (each group: 25 females, mean ages ~61 years).Field Strength/Sequence3‐T, two‐dimensional (2D) MESE sequence.AssessmentFemoral and tibial cartilages were segmented from DESS images, subdivided into seven sub‐regions, and co‐registered to MESE. T2 maps were obtained using three EPG‐based methods (nonlinear least squares, dictionary matching, and deep learning) and three mono‐exponential approaches (linear least squares, nonlinear least squares, and noise‐corrected exponential). Average T2 values within sub‐regions were obtained. Pair‐wise agreement among fitting methods was evaluated using the stratified subjects, while reproducibility using healthy subjects. Each method's T2 accuracy and repeatability varying signal‐to‐noise ratio (SNR) were assessed with simulations.Statistical TestsBland–Altman analysis, Lin's concordance coefficient, and coefficient of variation assessed agreement, repeatability, and reproducibility. Statistical significance was set at P‐value <0.05.ResultsEPG‐based methods demonstrated superior T2 accuracy (mean absolute error below 0.5 msec at SNR > 100) compared to mono‐exponential methods (error > 7 msec). EPG‐based approaches had better reproducibility, with limits of agreement 1.5–5 msec narrower than exponential‐based methods. T2 values from EPG methods were systematically 10–17 msec lower than those from mono‐exponential fitting.Data ConclusionEPG modeling improved agreement and reproducibility of cartilage T2 mapping in subjects from the OAI dataset.Evidence Level3Technical EfficacyStage 1
NMPC‐Based Control of Underactuated Spacecraft: A Neighboring Extremal Approach Extended to SE(3)
Authors: Bagherzadeh Shiva , Karimpour Hossein , Keshmiri Mehdi 
Published: October 2024

ABSTRACTThe present work revolves around employing the benefits of neighboring extremal approach in the nonlinear model predictive control of mechanical systems evolving on SE(3). In the NMPC process, necessary conditions of optimality are extracted based on some discrete‐time version of the equations of motion referred as LGVI and the obtained TPBVP equations are solved using simplified sensitivity derivatives by means of an indirect shooting method. Taking into consideration that the repetitive optimization process of the NMPC is time consuming, making its implementation challenging, a method of neighboring extremal is proposed here for recalculating the responses of the systems in scenarios that face some unexpected changes in their parameters or are encountering some last‐minute re‐planning schemes. Based on the existing responses of the system to the first set of initial conditions, the reaction of the system to the altered set of initial conditions can be reconstructed without the need to solve the whole optimization process from scratch by utilizing the features of the NE method. A spacecraft model evolving on SE(3) with actuation constraints confirms the efficiency of the whole process in term of accuracy versus computation burden. Forasmuch as actuator failure is a probable yet important event in aerial/spatial missions, the performance of the control system in dealing with such situations is examined. The algorithm robustness and its capability to compensate the effects of the actuator loss is checked.