bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reactive oxygen species prevent lysosome coalescence during PIKfyve inhibition
Authors: Saffi Golam T. , Tang Evan , Inpanathan Subothan , Mammad Sami , Fountain Aaron , Salmena Leonardo , Botelho Roberto J. 
Published: July 2019

AbstractLysosomes are terminal, degradative organelles of the endosomal pathway that undergo repeated fusion-fission cycles with themselves, endosomes, phagosomes, and autophagosomes. Lysosome number and size depends on balanced fusion and fission rates. Thus, conditions that favour fusion over fission can reduce lysosome numbers while enlarging their size. Conversely, favouring fission over fusion may cause lysosome fragmentation and increase their numbers. PIKfyve is a phosphoinositide kinase that generates phosphatidylinositol-3,5-bisphosphate to modulate lysosomal functions. PIKfyve inhibition causes an increase in lysosome size and reduction in lysosome number, consistent with lysosome coalescence. This is thought to proceed through reduced lysosome reformation and/or fission after fusion with endosomes or other lysosomes. Previously, we observed that photo-damage during live-cell imaging prevented lysosome coalescence during PIKfyve inhibition. Thus, we postulated that lysosome fusion and/or fission dynamics are affected by reactive oxygen species (ROS). Here, we show that ROS generated by various independent mechanisms all impaired lysosome coalescence during PIKfyve inhibition and accelerated lysosome fragmentation during re-activation. However, depending on the ROS species or mode of production, lysosome dynamics were affected distinctly. H2O2 impaired lysosome motility and reduced lysosome fusion with phagosomes, suggesting that H2O2 reduces lysosome fusogenecity. In comparison, inhibitors of oxidative phosphorylation, glutathione, and thioredoxin that produce superoxide, did not impair lysosome motility but instead promoted clearance of actin puncta on lysosomes formed during PIKfyve inhibition. Additionally, actin depolymerizing agents prevented lysosome coalescence during PIKfyve inhibition. Thus, we discovered that ROS can generally prevent lysosome coalescence during PIKfyve inhibition using distinct mechanisms depending on the type of ROS.
Membrane proximal F-actin restricts local membrane protrusions and directs cell migration
Authors: Bisaria Anjali , Hayer Arnold , Garbett Damien , Cohen Daniel , Meyer Tobias 
Published: July 2019

AbstractA major component of cell migration is F-actin polymerization driven membrane protrusion in the front. However, F-actin proximal to the plasma membrane also has a scaffolding role to support and attach the membrane. Here we developed a fluorescent reporter to monitor changes in the density of membrane proximal F-actin during membrane protrusion and cell migration. Strikingly, unlike total F-actin concentration, which is high in the front of migrating cells, the density of membrane proximal F-actin is low in the front and high in the back. Furthermore, local membrane protrusions only form following local decreases in membrane proximal F-actin density. Our study suggests that low density of membrane proximal F-actin is a fundamental structural parameter that locally directs membrane protrusions and globally stabilizes cell polarization during cell migration.One Sentence SummaryMembrane protrusion and cell migration are directed by local decreases in the density of membrane proximal F-actin
Evaluation of 21 Brassica microgreens growth and nutritional profile grown under diffrenet red, blue and green LEDs combination
Authors: Kamal Khaled Y. , El-Tantawy Ahmed A. , Moneim Diaa Abdel , Salam Asmaa Abdel , Qabil Naglaa , Ash-shormillesy Salwa M. A. I. , Attia Ahmed , Ali Mohamed A. S. , Herranz Raúl , El-Esawi Mohamed A. , Nassrallah Amr A. 
Published: July 2019

AbstractMicrogreens are rich functional crops with valuable nutritional elements that have health benefits when used as food supplants. Growth characterization, nutritional composition profile of 21 varieties representing 5 species of the Brassica genus as microgreens were assessed under light-emitting diodes (LEDs) conditions. Microgreens were grown under four different LEDs ratios (%) (R80:B20, R20:B80, R70:G10:B20, and R20:G10:B70). Results indicated that supplemental lighting with green LEDs (R70:G10:B20) enhanced vegetative growth and morphology, while blue LEDs (R20:B80) increased the mineral composition and vitamins content. Interestingly, combining the nutritional content with the growth yield to define the optimal LEDs setup, we found that the best lighting to promote the microgreen growth was supplying the green LEDs combination (R70:G10:B20). Remarkably, under this proper conditions, Kohlrabi purple, Cabbage red, Broccoli, Kale Tucsan, Komatsuna red, Tatsoi, and Cabbage green had the highest growth and nutritional content profile as microgreens which being a health-promoting in a diet support strategy required for the human health under certain isolated of limited food conditions.
Bilateral auditory processing studied by selective cold-deactivation of cricket hearing organs
Authors: Zhang Xinyang , Hedwig Berthold 
Published: July 2019

ABSTRACTWe studied bilateral processing in the auditory ON neurons of crickets using reversible cold-deactivation of the hearing organs by means of Peltier elements. Intracellular recordings of the neurons’ activity in response to acoustic stimuli were obtained, while either the ipsilateral or the contralateral hearing organ was cold-deactivated. Afferent activity was abolished at a temperature of about 10°C. In ON1 contralateral inhibition has no effect on the latency and amplitude of the phasic onset activity, it enhances the decline of the onset activity and decreases the subsequent tonic spiking response to acoustic stimuli. As a consequence the phasic onset activity becomes more salient and reciprocal inhibition may support the detection of sound pulses. Contralateral inhibition had a significant impact on the tonic ON1 response, in line with its presumed function to enhance the bilateral auditory contrast. In ON2, experiments confirmed a bilateral excitatory input, with the ipsilateral input dominating the response, and no inhibitory coupling between the ON2 neurons.
Examining cultural impacts on consumers’ environmental CSR outcomes
Authors: Bhalla Nandini , Overton Holly K. 
Published: July 2019

PurposeThe purpose of this paper is to examine the impact of cultural factors on environmental CSR attitudes and purchase intentions among publics in a developed (USA) country and a developing (India) country.Design/methodology/approachUsing a 2 (location of the company: India vs USA) × 2 (location of the CSR activity: India vs USA) between-subjects experimental design, the study examines individuals’ attitudes and behavioral intentions toward a company’s environmental CSR activities in each respective country.FindingsTwo structural equation models were created for US publics and Indian publics. Results indicated that cultural factors, specifically the uncertainty avoidance dimension, play an important role among both Indian and US residents’ attitudes toward a company’s environmental CSR efforts and their intention to purchase its products/services. Among Indians, the power distance dimension acted as a mediating factor.Originality/valueThis study is novel in its examination of the impact of cultural factors among residents in India and the USA. This information can be utilized by multinational companies to implement effective CSR activities and enhancing their global CSR communication efforts.
Facilitating or inhibiting?
Authors: Chen Hui , Liang Qiao-zhuan , Zhang Yue 
Published: July 2019

PurposeThe current research studies are inconclusive about the positive or negative effects of group faultlines, especially in the Chinese context. To address this issue, this study aims to adopt an interactive perspective to explore the group interaction process. Specifically, this study proposes a new construct “interactive faultlines” to integrate overall faultlines and separate faultlines, and based on categorization-elaboration model (CEM), develops an integrated moderated mediation model to examine when and how interactive faultlines facilitate or inhibit group creativity.Design/methodology/approachThis study tests the model with the samples of 405 employees from 95 groups in China, carrying out confirmatory factor analysis, regression analysis and process.FindingsThis study finds that the indirect effect of informational faultlines on group creativity through information elaboration is positive when social faultlines are low, but negative when social faultlines are high.Practical implicationsThis research provides some practical implications on how to manage group compositions and coordinate group interaction process to make full use of the potential benefits of diverse information and avoid the possible detriment from social categorization.Originality/valueThis study adopts an interactive perspective to consider informational faultlines and social faultlines simultaneously, and constructs a focal concept “interactive faultlines.” Based on CEM, it also offers a fine-grained picture of the double-edged relationship between informational faultlines and group creativity by identifying social faultlines as a moderator and information elaboration as a mediator, which advances knowledge about the linkages between interactive faultlines and group creativity. Particularly, this study is rooted in the Chinese context and brings in indigenous attributes derived from an analysis of Eastern cultures to elucidate the particular effect of informal social connections.
Site managers’ ICT tools for monitoring resources in refurbishment
Authors: Sezer Ahmet Anıl , Bröchner Jan 
Published: July 2019

Purpose
The purpose of this paper is to analyse site managers’ ICT preferences for monitoring resource use in refurbishment projects.

Design/methodology/approach
Information and communication technologies (ICT) developments for the construction industry are increasing the scope for more efficient planning and monitoring of refurbishment projects. The analysis is based on the unified theory of acceptance and the use of technology model. After a short initial survey, a Swedish web/postal questionnaire has received 78 responses from refurbishment site managers, implying a 34 per cent response rate.

Findings
Managerial choices related to ICT depend more on perceived performance expectancy than on effort expectancy. Large projects and larger firms are associated with more extensive ICT use. Site managers see little need to link to refurbishment clients’ ICT systems. Performance expectancy and age are found to influence ICT choices.

Practical implications
Site managers play a crucial role in everyday use of ICT tools in the construction industry. The outcome of this investigation is useful for developing digital support, including applications of building information modelling, to improve refurbishment site practices.

Originality/value
Much has been written about ICT support for new construction practices, but not much attention has been paid to refurbishment site managers’ media choices.
Cultural orientation, perceived support and participation of female students in formal entrepreneurship in the sub-Saharan economy of Ghana
Authors: Anlesinya Alex , Adepoju Oluwayemisi Ajoke , Richter Ulf Henning 
Published: July 2019

Purpose
This purpose of this paper is to examine cultural orientations and intention of Ghanaian women to engage in entrepreneurship while assessing the role of perceived support system. The aim is to contribute to the literature in the sub-Saharan African context where women entrepreneurs are generally under-researched, despite their increasing significant roles in socio-economic development in the continent even in the face of huge cultural barriers.

Design/methodology/approach
The study uses a hierarchical regression analysis and Hay’s PROCESS moderation technique to analyze survey data from 190 female students from Ghana, Africa.

Findings
The results indicate that uncertainty avoidance and power distance cultural orientations have significant positive and negative effects, respectively, on women’s participation in formal entrepreneurship. However, collectivism and masculine cultural orientations do not have any effect on their intention to engage in formal entrepreneurial activity. The study further shows that perceived support system has a buffering effect on the destructive consequences of power distance culture on formal entrepreneurship intentions. On the contrary, perceived support does not moderate the relationship between uncertainty avoidance, collectivism and masculine cultural and formal entrepreneurial intention.

Practical implications
Given the fact that most African governments are making efforts to accelerate the growth and development of their economies via entrepreneurship and economic empowerment, this study’s findings encourage stakeholders to implement measures to leverage on the positive dimensions of cultures to facilitate the development of formal entrepreneurship among Ghanaian women while mitigating the negative consequences of cultural practices. The findings further highlight the need to evaluate the current level of support given to women in Ghana. The study suggests that provision of sufficient level of support can make women more willing to challenge the status quo in power distance cultures and take personal initiatives, thereby leading to more formal entrepreneurial actions.

Originality/value
This study is a significant addition to women entrepreneurship literature because the role of culture in females’ intention to participate in entrepreneurship is generally an under-researched area. Besides, our examination of national cultural variation at the individual level on formal entrepreneurship intention in a heterogeneous setting is novel. The study also highlights the buffering roles of perceived support on the destructive consequences of power distance cultural orientation on formal entrepreneurial development among women.
Technology adoption and gender-inclusive entrepreneurship education and training
Authors: Orser Barbara , Riding Allan , Li Yanhong 
Published: July 2019

PurposeDrawing on social feminist theory, this paper aims to close gaps between knowledge about gender-related barriers to information, communication and technology (ICT) adoption and the provision of entrepreneurship education and training (EET) programs.Design/methodology/approachEmpirical findings are drawn from 21 semi-structured interviews (22 informants) possessing differing training expertise regarding digital technology among women entrepreneurs. An open-coding technique was adopted where descriptive codes were first assigned to meaningful statements. Interpretive and pattern codes were then assigned to indicate common themes and patterns, which were reduced to higher-order categories to inform the research questions.FindingsThe findings specify and validate further gender influences in the digital economy. Digital skills are identified, and strategies to close gender barriers to ICT adoption with EET are described. The findings are discussed in reference to a large-scale, Canadian ICT adoption program.Research limitations/implicationsPerceptual data may be idiosyncratic to the sample. The work did not control for type of technology. Gender influences may differ by type of technology.Practical implicationsFindings can be used to construct gender-inclusive ICT supports and inform ICT adoption policies. This includes program eligibility and evaluation criteria to measure the socio-economic impacts.Originality/valueThe study is among the first to examine the intersection between knowledge about gender-related barriers to ICT adoption and EET. The findings can be adopted to ICT support programs targeted at small business owners and entrepreneurs.
Lesson Study effectiveness for teachers’ professional learning: a best evidence synthesis
Authors: Willems Iris , Van den Bossche Piet 
Published: July 2019

PurposeThe purpose of this paper is to gather evidence for the effectiveness of Lesson Study (LS) for teachers’ professional learning.Design/methodology/approachA systematic review of relevant papers published between January 2010 and April 2018 was performed. To accomplish the best evidence synthesis, relevant studies were selected based upon well-chosen keywords and inclusion criteria. Afterwards, the quality of the remaining studies was assessed by using a critical appraisal checklist combined with a strength and weakness analysis. Data sources included relevant articles identified through digital searches on Education Research Information Centre and Web of Science, as well through snowball sampling and personal contacts. A total of five studies meeting inclusion criteria were retained for this review.FindingsThese five studies describe LS as a powerful professional development approach because of its positive impact on teachers’ professional learning. A significant improvement or change for knowledge, skills, behaviour and beliefs has been reported among teachers.Originality/valueOnly studies with a design that shows high effectiveness were selected. The sample of relevant studies is currently small. As a consequence more large-scale and long-term research which illustrates the short and long-term effects of the LS approach on teachers’ and students’ learning is desirable.
Finite element approaching analysis of load localization and identification for smart structures
Authors: Li Hua , Jia Lufeng 
Published: July 2019

Purpose
The purpose of this paper is to propose a numerical approaching analysis method combining the sequential unconstrained minimization technique and finite element method to identify the loading condition and geometry of smart structures accurately.

Design/methodology/approach
A new load identification model is built and the finite element approaching method is proposed by the combination of finite element method and optimization technique.

Findings
The approaching algorithm has good convergence and fast approximation speed; the accuracy can meet the engineering requirements. The approaching model is simple, and the precision is controllable and it can be used to solve the load identification problem of the smart material structure.

Originality/value
In view of the cited papers, the information sensed by the smart structure is limited, discrete and contains certain errors. How to derive the cause from the limited, error-containing discrete information is an important problem that needs to be solved by the self-diagnosis function. A load identification model based on structural displacement response is established and a numerical approximation method is proposed by combining the finite element method with the optimization technique; the load magnitude and position of the structure are identified according to the displacement measurement values of the internal finite point in the structure under the load condition.
Analysis of loss in flexural stiffness of in-service prestressed hollow plate beam
Authors: Zhang Guanhua , Wang Jiawei , Liu Jinliang , Jia Yanmin , Han Jigang 
Published: July 2019

Purpose
During service, cracks are caused in prestressed concrete beams owing to overload or other non-load factors. These cracks significantly affect the safety of bridge structures. The purpose of this paper is to carry out a non-linear iterative calculation for a section of a prestressed concrete beam and obtain the change in stiffness after the section cracks.

Design/methodology/approach
The existing stress of prestressed reinforcement was measured by performing a boring stress release test on two pieces of an in-service 16 m prestressed concrete hollow plate. Considering the non-linear effects of materials, the calculation model of the loss in the flexural stiffness of the prestressed concrete beam was established based on the existing prestress. The accuracy of the non-linear calculation method and the results obtained for the section were verified by conducting a bending destruction test on two pieces of the 16 m prestressed concrete hollow plate in the same batch and by utilising the measured strain and displacement data on the concrete at the top edge of the midspan section under all load levels.

Findings
The flexural stiffness of the section decreases rapidly at first and then gradually, and structural rigidity is sensitive to the initial cracking of the beam. The method for calculating the loss in the flexural stiffness of the section established with the existing stress of prestressed reinforcement as a parameter is accurate and feasible. It realizes the possibility of assessing the loss in the rigidity of a prestressed concrete structure by adopting the existing stress of prestressed reinforcement as a parameter.

Originality/value
A method for quickly determining the loss in the stiffness of structures using existing prestress is established. By employing this method, engineers can rapidly determine whether a bridge is dangerous or not without performing a loading test. Thus, this method not only ensures the safety of human life, but also reduces the cost of testing.
Contact modeling with a finite element model in piston ring‒liner conjunction under dry conditions
Authors: Nikolakopoulos Pantelis G. , Grigoriadis Kyriakos , Zavos Anastasios 
Published: July 2019

Purpose
The purpose of this paper is to focus on the creation of an isothermal elastic ring-liner model to highlight, through stresses, the occurrence of the plastic deformation in certain crank angles under extreme dry lubrication conditions.

Design/methodology/approach
The stresses that are exported from this analysis are pointing out not only the necessity for an elastoplastic model to be created, but also the importance of predicting the correct friction coefficient, as pointed out by both the contact surface stress and that in depth of the two bodies in contact.

Findings
The comparison between the finite element model and the adhesion mathematical model of Johnson, Kendall and Roberts seals the importance to calculate the interaction forces, acting on the common solid surface, in the pursuit of defining a propriate contact patch. Additionally, a three-dimensional ring model is built, highlighting the importance of the modeling surface’s micro asperities for a solid stress analysis. Also, numerical experiments are conducted, in contact with the cylinder and a piston ring made of an iron alloy and of two different plating materials, such us Chromium (Cr) and Chromium‒Nickel Alloy (CrN). The ability to calculate the stress concentration factor is also described.

Originality/value
A three-dimensional ring model is built, highlighting the importance of the modeling surface’s micro asperities for a solid stress analysis. Also, numerical experiments are conducted, in contact with the cylinder and a piston ring made of an iron alloy and of two different plating materials, such us Chromium (Cr) and Chromium‒Nickel Alloy (CrN). The ability to calculate the stress concentration factor is also described.