bluuebunny/crossref_metadata_2025_split huggingface dataset:
N‐glycosylation by N‐acetylglucosaminyltransferase V enhances the interaction of CD147/basigin with integrin β1 and promotes HCC metastasis
Authors: Cui Jian , Huang Wan , Wu Bo , Jin Jin , Jing Lin , Shi Wen‐Pu , Liu Zhen‐Yu , Yuan Lin , Luo Dan , Li Ling , Chen Zhi‐Nan , Jiang Jian‐Li 
Published: February 2018

AbstractWhile the importance of protein N‐glycosylation in cancer cell migration is well appreciated, the precise mechanisms by which N‐acetylglucosaminyltransferase V (GnT‐V) regulates cancer processes remain largely unknown. In the current study, we report that GnT‐V‐mediated N‐glycosylation of CD147/basigin, a tumor‐associated glycoprotein that carries β1,6‐N‐acetylglucosamine (β1,6‐GlcNAc) glycans, is upregulated during TGF‐β1‐induced epithelial‐to‐mesenchymal transition (EMT), which correlates with tumor metastasis in patients with hepatocellular carcinoma (HCC). Interruption of β1,6‐GlcNAc glycan modification of CD147/basigin decreased matrix metalloproteinase (MMP) expression in HCC cell lines and affected the interaction of CD147/basigin with integrin β1. These results reveal that β1,6‐branched glycans modulate the biological function of CD147/basigin in HCC metastasis. Moreover, we showed that the PI3K/Akt pathway regulates GnT‐V expression and that inhibition of GnT‐V‐mediated N‐glycosylation suppressed PI3K signaling. In summary, β1,6‐branched N‐glycosylation affects the biological function of CD147/basigin and these findings provide a novel approach for the development of therapeutic strategies targeting metastasis. © 2018 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
Discrimination of Cynanchum wilfordii and Cynanchum auriculatum by terahertz spectroscopic analysis
Authors: Ham Woo Sik , Kim Jinju , Park Dae Joon , Ryu Han‐Cheol , Jang Young Pyo 
Published: February 2018

AbstractIntroductionPrecise identification of botanical origin of plant species is crucial for the quality control of herbal medicine. In Korea, the root part of Cynanchum auriculatum has been misused for C. wilfordii in the herbal drug market due to their morphological similarities. Currently, DNA analysis using the polymerase chain reaction (PCR) method is employed to discriminate between these species.ObjectiveIn order to develop a new analytical tool for the rapid discrimination of C. wilfordii and C. auriculatum, terahertz (THz) spectroscopy was employed.MethodologyAuthentic samples of C. wilfordii and C. auriculatum were provided from the National Institute and standardized pellets for each species were prepared to get optimum results with terahertz time‐domain spectroscopy (THz‐TDS) in frequency range 0.2–1.20 THz.ResultsThe C. wilfordii pellet showed longer time delay compare to the sample of C. auriculatum and this was due to the difference in permittivity. The pellet samples of C. wilfordii and C. auriculatum showed a permittivity difference of about 0.08 at 0.2–1.20 THz.ConclusionThe experimental results indicated that THz‐TDS analysis can be an effective and rapid method for the discrimination of C. wilfordii and C. auriculatum, and this application can be expanded for the discrimination of other similar herbal medicines.
Use of dye complexation dynamics to determine α‐cyclodextrin host:guest stability constants
Authors: Rodríguez María , Silva Lara , Parajó Mercedes , Rodríguez‐Dafonte Pedro , García‐Río Luis 
Published: February 2018

AbstractDynamics of host:dye complexation have been used, as an alternative to displacement assays, for determination of additional host:guest stability constants. Requirement of this new methodology is host:guest complexation being much faster than host:dye in order to guaranty that host:guest system is in equilibrium at each time during dye inclusion into the host cavity. Methodology was tested by studying α‐CD:surfactant complexation using alkyltrimethylammonium surfactants with different alkyl chain length. Experimental results confirm the formation of 1:1 α‐CD:surfactant complexes with binding constants strongly dependent on the surfactant hydrophobicity.
Expecting the worst? The relationship between retrospective and prospective appraisals of illness on quality of life in prostate cancer survivors
Authors: Maguire Rebecca , Hanly Paul , Drummond Frances J. , Gavin Anna , Sharp Linda 
Published: February 2018

AbstractObjectiveDespite a generally good prognosis, many prostate cancer survivors have poor quality of life (QOL). A greater understanding of how psychological appraisals influence QOL is merited given their potentially modifiable nature. In this study, we considered how elements of survivors' retrospective and prospective appraisals relate to QOL.MethodsA total of 1229 prostate cancer survivors between 2 and 5 years post‐diagnosis, identified from a population‐based National Cancer Registry, were asked questions on their socio‐demographics, health, treatment received, and adverse‐effects using a cross‐sectional design. QOL was assessed using the EORTC QLQ‐C30. Retrospective appraisals were assessed by asking survivors to reflect on their experience of treatment‐related adverse‐effects compared with their prior expectations. A fear of recurrence scale assessed prospective appraisals of future disease course. A multiple regression model explored the impact of psychological appraisals on QOL, after controlling for socio‐demographic, treatment, and health‐related factors.ResultsThe model was significant explaining 37% of variance in QOL. The strongest associate with QOL was fear of recurrence (β = −.29; P < .001). Survivors who experienced side effects that were worse than expected had significantly lower QOL (β = −.10; P = .002). Other significant correlates of lower QOL were presence of comorbidities, having undergone a less invasive treatment, and having more advanced disease. Working at diagnosis and having a higher level of education were significantly associated with higher QOL.ConclusionsResults suggest both retrospective and prospective appraisals are independently related to QOL in prostate cancer. Providing survivors with more information about possible adverse effects of treatment, as well as providing appropriate information regarding future disease progression, may improve QOL.
Factors associated with romantic relationship formation difficulties in women with breast cancer
Authors: Shaw L.‐K. , Sherman K.A. , Fitness J. , Elder E. ,   Breast Cancer Network Australia
Published: February 2018

AbstractObjectiveMany un‐partnered women report difficulty in forming romantic relationships after breast cancer, characterized by high dating‐related anxiety and low perceived interpersonal competence. This study examined the relationship between poor body image (appearance investment and body dissatisfaction) and self‐compassion, and women's ability to form romantic relationships post‐breast cancer.MethodsWomen (N = 152) diagnosed with breast cancer, who were either un‐partnered and expressed interest in romantic dating, or who had commenced a relationship post‐diagnosis, completed an online survey. Assessments included the Interpersonal Competence Questionnaire, Dating Anxiety Scale, Self‐compassion Scale, Appearance Schemas Inventory‐Revised, Body Image Scale, and Experiences in Close Relationships Scale. Multiple regression analyses assessed the relationships between these variables.ResultsPartnered and un‐partnered women differed in levels of dating anxiety, interpersonal competence, anxious attachment, and the self‐evaluative salience facet of appearance investment. Analyses revealed a significant model for dating anxiety, with high self‐evaluative salience, body image dissatisfaction, and attachment avoidance independently associated with this outcome. The model for interpersonal competence was also significant, with low attachment avoidance and high self‐compassion independently associated with this outcome.ConclusionsUn‐partnered women who place high importance on appearance for their self‐worth and who report poor body image and low self‐compassion are at risk of experiencing difficulties in forming new romantic relationships after breast cancer. Future interventions should target these variables to facilitate romantic dating during cancer survivorship.
Solution structure of the PHD finger from the human KIAA1045 protein
Authors: Miyamoto Kazuhide , Yamashita Ayumi , Saito Kazuki 
Published: February 2018

AbstractCross‐brace structural motifs are required as a scaffold to design artificial RING fingers (ARFs) that function as ubiquitin ligase (E3) in ubiquitination and have specific ubiquitin‐conjugating enzyme (E2)‐binding capabilities. The Simple Modular Architecture Research Tool database predicted the amino acid sequence 131–190 (KIAA1045ZF) of the human KIAA1045 protein as an unidentified structural region. Herein, the stoichiometry of zinc ions estimated spectrophotometrically by the metallochromic indicator revealed that the KIAA1045ZF motif binds to two zinc atoms. The structure of the KIAA1045ZF motif bound to the zinc atoms was elucidated at the atomic level by nuclear magnetic resonance. The actual structure of the KIAA1045ZF motif adopts a C4HC3‐type PHD fold belonging to the cross‐brace structural family. Therefore, the utilization of the KIAA1045ZF motif as a scaffold may lead to the creation of a novel ARF.
Circulating tumor cells and survival in abiraterone‐ and enzalutamide‐treated patients with castration‐resistant prostate cancer
Authors: De Laere Bram , Oeyen Steffi , Van Oyen Peter , Ghysel Christophe , Ampe Jozef , Ost Piet , Demey Wim , Hoekx Lucien , Schrijvers Dirk , Brouwers Barbara , Lybaert Willem , Everaert Els , Van Kerckhove Piet , De Maeseneer Daan , Strijbos Michiel , Bols Alain , Fransis Karen , Beije Nick , de Kruijff Inge , van Dam Valerie , Brouwer Anja , van Dam Pieter‐Jan , Van den Eynden Gert , Rutten Annemie , Sleijfer Stefan , Vandebroek Jean , Van Laere Steven , Dirix Luc 
Published: February 2018

BackgroundThe outcome to treatment administered to patients with metastatic castration‐resistant prostate cancer (mCRPC) greatly differs between individuals, underlining the need for biomarkers guiding treatment decision making.ObjectiveTo investigate the prognostic value of circulating tumor cell (CTC) enumeration and dynamics, in the context of second‐line endocrine therapies (ie, abiraterone acetate or enzalutamide), irrespective of prior systemic therapies.Design, Settings, and ParticipantsIn a prospective, multicentre study blood samples for CTC enumeration were collected from patients with mCRPC at baseline (n = 174). In patients who responded for minimally 10‐12 weeks a follow‐up sample was collected.Outcome Measurements and Statistical AnalysisFor baseline analysis, patients were stratified in <5 or ≥5 CTCs/7.5 mL, whereas for the analysis of CTC dynamics at 10‐12 weeks, in patients with stable, increasing or decreasing CTC counts. Progression‐free survival (PFS), overall survival (OS), and PSA changes at 10‐12 weeks were compared between groups.ResultsPatients demonstrating increasing CTCs on therapy had a shorter median PFS (4.03 vs 12.98 vs 13.67 months, HR 3.6, 95%CI 1.9‐6.8; P < 0.0001) and OS (11.2 months vs not reached, HR 9.5, 95%CI 3.7‐24; P < 0.0001), compared to patients with decreasing or stable CTCs. Multivariable Cox regression showed that prior chemotherapy (HR 4.1, 95%CI 1.9‐8.9; P = 0.0003), a high baseline CTC count (HR 1.5, 95%CI 1.2‐1.9; P = 0.002) and increasing CTCs at follow‐up (HR 3.3, 95%CI 1.4‐7.6; P = 0.005) were independent predictors of worse PFS. Previous chemotherapy (HR 7, 95%CI 1.9‐25; P = 0.003), high baseline CTC counts (HR 2.2, 95%CI 1.4‐3.7; P = 0.002) and increasing CTCs during therapy (HR 4.6, 95%CI 1.4‐15; P = 0.01) were independently associated with shorter OS. ≥30% and ≥50% PSA responses less frequently occurred in patients with CTC inclines at 10‐12 weeks on therapy (χ2 test: P < 0.01).ConclusionsCTC dynamics during therapy are associated with PSA response and provide independent clinical prognostication over PSA declines. Hence the study demonstrates the pharmacodynamic properties of CTCs.
Thermodynamic properties of diatomic molecule systems under SO(2,1)‐anharmonic Eckart potential
Authors: Valencia‐Ortega Gabriel , Arias‐Hernandez Luis‐Antonio 
Published: February 2018

AbstractDue to one of the most representative contributions to the energy in diatomic molecules being the vibrational, we consider the generalized Morse potential (GMP) as a typical interaction for one‐dimensional microscopic systems, which describes local anharmonic effects. From the Eckart potential (EP) model, it is possible to find a connection with the GMP model, as well as obtaining the analytical expression for the energy spectrum because it is based on
algebras. This gives the macroscopic properties such as vibrational mean energy U, specific heat C, Helmholtz free energy F, and entropy S for a heteronuclear diatomic system, as well as with the exact partition function and its approximation for the high temperature region. Finally, a comparison is made between the graphs of some thermodynamic functions obtained with the GMP and the Morse potential (MP) for
molecules.
Formamidinesulfinic Acid‐Functionalized Fe3O4@SiO2 as a Green and Magnetic Recyclable Catalyst for Synthesis of Pyrano[2, 3‐d] pyrimidinone Derivatives
Authors: Ghandi Leila , Miraki Maryam Kazemi , Radfar Iman , Yazdani Elahe , Heydari Akbar 
Published: February 2018

AbstractFormamidine sulfonic acid (FSA) stabilized on silica‐coated Fe3O4 magnetic nanoparticles as an efficient and hydrogen bonding catalyst was employed for Knoevenagel condensation and synthesis of pyrano[2, 3‐d]pyrimidinone derivatives by the one‐pot three‐component condensation reaction in water at room temperature. The catalyst can be separated from the mixture reaction which is in close agreement with green chemistry disciplines.
Dynamically PEGylated and Borate‐Coordination‐Polymer‐Coated Polydopamine Nanoparticles for Synergetic Tumor‐Targeted, Chemo‐Photothermal Combination Therapy
Authors: Liu Shucheng , Pan Jianming , Liu Jinxin , Ma Yue , Qiu Fengxian , Mei Lin , Zeng Xiaowei , Pan Guoqing 
Published: February 2018

AbstractMultifunctional nanomaterials with efficient tumor‐targeting and high antitumor activity are highly anticipated in the field of cancer therapy. In this work, a synergetic tumor‐targeted, chemo‐photothermal combined therapeutic nanoplatform based on a dynamically PEGylated, borate‐coordination‐polymer‐coated polydopamine nanoparticle (PDA@CP‐PEG) is developed. PEGylation on the multifunctional nanoparticles is dynamically achieved via the reversible covalent interaction between the surface phenylboronic acid (PBA) group and a catechol‐containing poly(ethylene glycol) (PEG) molecule. Due to the acid‐labile PBA/catechol complex and the weak‐acid‐stable PBA/sialic acid (SA) complex, the nanoparticles can exhibit a synergetic targeting property for the SA‐overexpressed tumor cells, i.e., the PEG‐caused “passive targeting” and PBA‐triggered “active targeting” under the weakly acidic tumor microenvironment. In addition, the photothermal effect of the polydopamine core and the doxorubicin‐loading capacity of the porous coordination polymer layer endow the nanoparticles with the potential for chemo‐photothermal combination therapy. As expected, the in vitro and in vivo studies both verify that the multifunctional nanoparticles possess relatively lower systematic toxicity, efficient tumor targeting ability, and excellent chemo‐photothermal activity for tumor inhibition. It is believed that these multifunctional nanoparticles with synergetic tumor targeting property and combined therapeutic strategies would provide an insight into the design of a high‐efficiency antitumor nanoplatform for potential clinical applications.
Prescribing 6-weeks of running training using parameters from a self-paced maximal oxygen uptake protocol
Authors: Hogg James S. , Hopker James G. , Coakley Sarah L. , Mauger Alexis R. 
Published: February 2018

Abstract
Purpose
The self-paced maximal oxygen uptake test (SPV) may offer effective training prescription metrics for athletes. This study aimed to examine whether SPV-derived data could be used for training prescription.

Methods
Twenty-four recreationally active male and female runners were randomly assigned between two training groups: (1) Standardised (STND) and (2) Self-Paced (S-P). Participants completed 4 running sessions a week using a global positioning system-enabled (GPS) watch: 2 × interval sessions; 1 × recovery run; and 1 × tempo run. STND had training prescribed via graded exercise test (GXT) data, whereas S-P had training prescribed via SPV data. In STND, intervals were prescribed as 6 × 60% of the time that velocity at $$\dot {V}{{\text{O}}_{{\text{2max}}}}$$

V
˙

O
2max

($$_{{\text{v}}}\dot {V}{{\text{O}}_{{\text{2max}}}}$$

v

V
˙

O
2max

) could be maintained (Tmax). In S-P, intervals were prescribed as 7 × 120 s at the mean velocity of rating of perceived exertion 20 (vRPE20). Both groups used 1:2 work:recovery ratio. Maximal oxygen uptake ($$\dot {V}{{\text{O}}_{{\text{2max}}}}$$

V
˙

O
2max

), $$_{{\text{v}}}\dot {V}{{\text{O}}_{{\text{2max}}}}$$

v

V
˙

O
2max

, Tmax, vRPE20, critical speed (CS), and lactate threshold (LT) were determined before and after the 6-week training.

Results
STND and S-P training significantly improved $$\dot {V}{{\text{O}}_{{\text{2max}}}}$$

V
˙

O
2max

by 4 ± 8 and 6 ± 6%, CS by 7 ± 7 and 3 ± 3%; LT by 5 ± 4% and 7 ± 8%, respectively (all P < .05), with no differences observed between groups.

Conclusions
Novel metrics obtained from the SPV can offer similar training prescription and improvement in $$\dot {V}{{\text{O}}_{{\text{2max}}}}$$

V
˙

O
2max

, CS and LT compared to training derived from a traditional GXT.
Characterization of Loading Responses and Failure Loci of a Boron Steel Spot Weld
Authors: Raath N. D. , Norman D. , McGregor I. , Hepple S. , Dashwood R. , Hughes D. J. 
Published: February 2018

Abstract
Boron steel, classed as an ultra high-strength steel (UHSS), has been utilized in anti-intrusion systems in automobiles, providing high strength and weight-saving potential through gage reduction. UHSS spot welds exhibit unique hardness distributions, with a hard nugget and outlying base material, but with a soft heat-affected zone in-between these regions. This soft zone reduces the strength of the weld and makes it susceptible to failure. Due to the interaction of various weld zones that occurs during loading, there is a need to characterize the loading response of the weld for accurate failure predictions. The loading response of certain weld zones, as well as failure loci, was obtained through physical simulation of the welding process. The results showed a significant difference in mechanical behavior through the weld length. An important result is that instrumented indentation was shown to be a valid, quantitative method for verifying the accuracy with which weld microstructure has been recreated with regard to the target weld microstructure.
Study of Solidification Cracking in a Transformation-Induced Plasticity-Aided Steel
Authors: Agarwal G. , Kumar A. , Gao H. , Amirthalingam M. , Moon S. C. , Dippenaar R. J. , Richardson I. M. , Hermans M. J. M. 
Published: February 2018

Abstract

In situ high-temperature laser scanning confocal microscopy is applied to study solidification cracking in a TRIP steel. Solidification cracking was observed in the interdendritic region during the last stage of solidification. Atom probe tomography revealed notable enrichment of phosphorus in the last remaining liquid. Phase field simulations also confirm phosphorus enrichment leading to severe undercooling of more than 160 K in the interdendritic region. In the presence of tensile stress, an opening at the interdendritic region is difficult to fill with the remaining liquid due to low permeability and high viscosity, resulting in solidification cracking.
Healthy minds 0–100 years: Optimising the use of European brain imaging cohorts (“Lifebrain”)
Authors: Walhovd Kristine B. , Fjell Anders M. , Westerhausen René , Nyberg Lars , Ebmeier Klaus P. , Lindenberger Ulman , Bartrés-Faz David , Baaré William F.C. , Siebner Hartwig R. , Henson Richard , Drevon Christian A. , Strømstad Knudsen Gun Peggy , Ljøsne Isabelle Budin , Penninx Brenda W.J.H. , Ghisletta Paolo , Rogeberg Ole , Tyler Lorraine , Bertram Lars ,   Lifebrain Consortium
Published: February 2018

AbstractThe main objective of “Lifebrain” is to identify the determinants of brain, cognitive and mental (BCM) health at different stages of life. By integrating, harmonising and enriching major European neuroimaging studies across the life span, we will merge fine-grained BCM health measures of more than 5000 individuals. Longitudinal brain imaging, genetic and health data are available for a major part, as well as cognitive and mental health measures for the broader cohorts, exceeding 27,000 examinations in total. By linking these data to other databases and biobanks, including birth registries, national and regional archives, and by enriching them with a new online data collection and novel measures, we will address the risk factors and protective factors of BCM health. We will identify pathways through which risk and protective factors work and their moderators. Exploiting existing European infrastructures and initiatives, we hope to make major conceptual, methodological and analytical contributions towards large integrative cohorts and their efficient exploitation. We will thus provide novel information on BCM health maintenance, as well as the onset and course of BCM disorders. This will lay a foundation for earlier diagnosis of brain disorders, aberrant development and decline of BCM health, and translate into future preventive and therapeutic strategies. Aiming to improve clinical practice and public health we will work with stakeholders and health authorities, and thus provide the evidence base for prevention and intervention.
ALIMENTAL FOOD CONSUMPTION AMONG URBAN HOUSEHOLDS: AN EMPIRICAL STUDY OF GHANA
Authors: MENG TING , FLORKOWSKI WOJCIECH J. , SARPONG DANIEL B. , CHINNAN MANJEET S. , RESURRECCION ANNA V.A. 
Published: February 2018

AbstractIn developing countries, problems such as malnutrition and food insecurity are shifting from rural to urban areas because of rapid urbanization. However, regional variations in alimental food consumption within urban settings have often been ignored. Using survey data, our study examines regional patterns of expenditure on fresh vegetables, fruits, and peanut products in urban households of Ghana. After accounting for socioeconomic and demographic factors, food expenditure on fresh vegetables and peanut products and income elasticity vary significantly across major cities. Food distributors may adjust their marketing strategies, while policy makers should pay attention to possible disparities in urban areas.