bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cbsoverdosage is necessary and sufficient to induce cognitive phenotypes in mouse models of Down syndrome and interacts genetically withDyrk1a
Authors: Marechal Damien , Brault Véronique , Leon Alice , Martin Dehren , Pereira Patricia Lopes , Loaёc Nadege , Birling Marie-Christine , Friocourt Gaelle , Blondel Marc , Herault Yann 
Published: August 2018

ABSTRACTIdentifying dosage sensitive genes is a key to understand the mechanisms underlying intellectual disability in Down syndrome (DS). The Dp(17Abcg1-Cbs)1Yah DS mouse model (Dp1Yah) show cognitive phenotype and needs to be investigated to identify the main genetic driver. Here, we report that, in the Dp1Yah mice, 3 copies of the Cystathionine-beta-synthase gene (Cbs)are necessary to observe a deficit in the novel object recognition (NOR) paradigm. Moreover, the overexpression ofCbsalone is sufficient to induce NOR deficit. Accordingly targeting the overexpression of human CBS, specifically in Camk2a-expressing neurons, leads to impaired objects discrimination. Altogether this shows thatCbsoverdosage is involved in DS learning and memory phenotypes. In order to go further, we identified compounds that interfere with the phenotypical consequence of CBS overdosage in yeast. Pharmacological intervention in the Tg(CBS) with one selected compound restored memory in the novel object recognition. In addition, using a genetic approach, we demonstrated an epistatic interaction betweenCbsandDyrk1a, another human chromosome 21 gene encoding the dual-specificity tyrosine phosphorylation-regulated kinase 1a and an already known target for DS therapeutic intervention. Further analysis using proteomic approaches highlighted several pathways, including synaptic transmission, cell projection morphogenesis, and actin cytoskeleton, that are affected by DYRK1A and CBS overexpression. Overall we demonstrated that CBS overdosage underpins the DS-related recognition memory deficit and that bothCBSandDYRK1Ainteract to control accurate memory processes in DS. In addition, our study establishes CBS as an intervention point for treating intellectual deficiencies linked to DS.SIGNIFICANT STATEMENTHere, we investigated a region homologous to Hsa21 and located on mouse chromosome 17. We demonstrated using three independent genetic approaches that the overdosage of the Cystathionine-beta-synthase gene (Cbs) gene, encoded in the segment, is necessary and sufficient to induce deficit in novel object recognition (NR).In addition, we identified compounds that interfere with the phenotypical consequence of CBS overdosage in yeast and in mouse transgenic lines. Then we analyzed the relation between Cbs overdosage and the consequence of DYRK1a overexpression, a main driver of another region homologous to Hsa21 and we demonstrated that an epistatic interaction exist betweenCbsandDyrk1aaffecting different pathways, including synaptic transmission, cell projection morphogenesis, and actin cytoskeleton.
Mitochondrial genome annotation and phylogenetic placement ofOreochromis andersoniiandO. macrochiramong the cichlids of southern Africa
Authors: Bbole Ian , Zhao Jin-Liang , Tang Shou-Jie , Katongo Cyprian 
Published: August 2018

AbstractGenetic characterization of southern African cichlids has not received much attention. Here, we describe the mitogenome sequences and phylogenetic positioning ofOreochromis andersoniiandO. macrochiramong the cichlids of southern Africa. The complete mitochondrial DNA sequences were determined forO. andersoniiandO. macrochir, two important aquaculture and fisheries species endemic to southern Africa. The complete mitogenome sequence lengths were 16642 bp and 16644 bp forO. andersoniiandO. macrochirrespectively. The general structural organization follows that of other teleost species with 13 protein–coding genes, 2rRNAs, 22tRNAsand a non-coding control region. Phylogenetic placement of the two species among other African cichlids was performed using Maximum Likelihood (ML) and Bayesian Markov-Chain-Monte-Carlo (MCMC). The consensus trees confirmed the relative positions of the two cichlid species withO. andersoniibeing very closely related toO. mossambicusandO. macrochirshowing a close relation to both species. Among the 13 mitochondrial DNA protein coding genesND6may have evolved more rapidly andCOIIIwas the most conserved. There are signs thatND6may have been subjected to positive selection in order for these cichlid lineages to diversity and adapt to new environments. More work is needed to characterize the southern Africa cichlids as they are important species for capture fisheries, aquaculture development and understanding biogeographic history of African cichlids. Bioconservation of some endangered cichlids is also essential due to the threat by invasive species.
Title: Determinants of Minimum Acceptable Diet among 6–23 Months Age Children in Ethiopia: A Multilevel Analysis of The Ethiopian Demographic Health Survey
Authors: abay Aberash , Yemane Dejen , Bekele Abate , Meressa Beyene 
Published: August 2018

AbstractBackgroundThough infant and young children should be fed according to a minimum acceptable diet to ensure appropriate growth and development, only 7% of Ethiopian 6-23 months age children meet the minimum acceptable dietary standards, which is lower than the national target of 11% set for 2016. Therefore, this study aims to assess the individual and community level determinants of minimum acceptable diet among 6–23 months age children in Ethiopia.MethodsThis study analyzed retrospectively a cross-sectional data on a weighted sample of 2919 children aged 6-23 months nested within 617 clusters after extracting from Ethiopian Demographic and Health Survey 2016 via the link www.measuredhs.com. By employing bi-variate multilevel logistic regression model, variables which were significant at the p-value < 25 were included in multivariable multilevel logistic regression analysis. Finally, variables with p-value < 0.05 were considered as significant predictors of minimum acceptable diet.ResultsOnly 6.1% of 6-23 months age children feed minimum acceptable diet in Ethiopia. Children 18-23 months age (AOR=3.7, 95%CI 1.9, 7.2), father’s with secondary or higher education (AOR=2.1, 95%CI 1.2, 3.6), Employed mothers (AOR=1.7, 95%CI 1.2, 2.5), mothers have access to drinking water (AOR=1.9, 95%CI 1.2, 2.9), mothers with media exposure (AOR=2.1 95%CI 1.1, 2.7) were positive individual level predictors. Urban mothers (AOR=4.8, 95%CI 1.7, 13.2)) and agrarian dominant region (AOR=5.6, 95%CI 2.2, 14.5) were community level factors that significantly associated with minimum acceptable diet of 6–23 months age children.ConclusionBoth individual and community level factors were significantly associated with minimum acceptable diet of 6-23 months age children in Ethiopia, suggesting that nutritional interventions designed to improve child health should not only be implemented at the individual level but tailored to community context as well.
Kinematics of wings fromCaudipteryxto modern birds
Authors: Talori Yaser Saffar , Zhao Jing-Shan , O’Connor Jingmai Kathleen 
Published: August 2018

AbstractThis study seeks to better quantify the parameters that drove the evolution of flight from nonvolant winged dinosaurs to modern birds. In order to explore this issue, we used fossil data to model the feathered forelimb ofCaudipteryx, the most basal non-volant maniraptoran dinosaur with elongate pennaceous feathers that could be described as forming proto-wings. In order to quantify the limiting flight factors, we created three hypothetical wing profiles forCaudipteryxrepresenting incrementally larger wingspans, which we compared to the actual wing morphology as what revealed through fossils. These four models were analyzed under varying air speed, wing beat amplitude, and wing beat frequency to determine lift, thrust potential and metabolic requirements. We tested these models using theoretical equations in order to mathematically describe the evolutionary changes observed during the evolution of modern birds from a winged terrestrial theropod likeCaudipteryx. Caudipteryxcould not fly, but this research indicates that with a large enough wing spanCaudipteryx-like animal could have flown, the morphology of the shoulder girdle would not actually accommodate the necessary flapping angle and metabolic demands would be much too high to be functional. The results of these analyses mathematically confirm that during the evolution of energetically efficient powered flight in derived maniraptorans, body weight had to decrease and wing area/wing profile needed to increase together with the flapping angle and surface area for the attachment of the flight muscles. This study quantifies the morphological changes that we observe in the pennaraptoran fossil record in the overall decrease in body size in paravians, the increased wing surface area inArchaeopteryxrelative toCaudipteryx, and changes observed in the morphology of the thoracic girdle, namely the orientation of the glenoid and the enlargement of the sternum.
Discovery of tandem and interspersed segmental duplications using high throughput sequencing
Authors: Soylev Arda , Le Thong , Amini Hajar , Alkan Can , Hormozdiari Fereydoun 
Published: August 2018

AbstractMotivationSeveral algorithms have been developed that use high throughput sequencing technology to characterize structural variations. Most of the existing approaches focus on detecting relatively simple types of SVs such as insertions, deletions, and short inversions. In fact, complex SVs are of crucial importance and several have been associated with genomic disorders. To better understand the contribution of complex SVs to human disease, we need new algorithms to accurately discover and genotype such variants. Additionally, due to similar sequencing signatures, inverted duplications or gene conversion events that include inverted segmental duplications are often characterized as simple inversions; and duplications and gene conversions in direct orientation may be called as simple deletions. Therefore, there is still a need for accurate algorithms to fully characterize complex SVs and thus improve calling accuracy of more simple variants.ResultsWe developed novel algorithms to accurately characterize tandem, direct and inverted interspersed segmental duplications using short read whole genome sequencing data sets. We integrated these methods to our TARDIS tool, which is now capable of detecting various types of SVs using multiple sequence signatures such as read pair, read depth and split read. We evaluated the prediction performance of our algorithms through several experiments using both simulated and real data sets. In the simulation experiments, using a 30× coverage TARDIS achieved 96% sensitivity with only 4% false discovery rate. For experiments that involve real data, we used two haploid genomes (CHM1 and CHM13) and one human genome (NA12878) from the Illumina Platinum Genomes set. Comparison of our results with orthogonal PacBio call sets from the same genomes revealed higher accuracy for TARDIS than state of the art methods. Furthermore, we showed a surprisingly low false discovery rate of our approach for discovery of tandem, direct and inverted interspersed segmental duplications prediction on CHM1 (less than 5% for the top 50 predictions).AvailabilityTARDIS source code is available at https://github.com/BilkentCompGen/tardis, and a corresponding Docker image is available at https://hub.docker.com/r/alkanlab/tardis/Contactfhormozd@ucdavis.edu and calkan@cs.bilkent.edu.tr
The core genome m5C methyltransferase JHP1050 (M.Hpy99III) plays an important role in orchestrating gene expression in Helicobacter pylori
Authors: Estibariz Iratxe , Overmann Annemarie , Ailloud Florent , Krebes Juliane , Josenhans Josenhans , Suerbaum Sebastian 
Published: August 2018

ABSTRACTHelicobacter pylori encodes a large number of Restriction-Modification (R-M) systems despite its small genome.R-M systems have been described as “primitive immune systems” in bacteria, but the role of methylation in bacterial gene regulation and other processes is increasingly accepted. Every H.pylori strain harbours a unique set of R-M systems resulting in a highly diverse methylome. We identified a highly conserved GCGC-specific m5C MTase (JHP1050) that was predicted to be active in all of 459 H.pylori genome sequences analyzed. Transcriptome analysis of two H.pylori strains and their respective MTase mutants showed that inactivation of the MTase led to changes in the expression of 225 genes in strain J99, and 29 genes in strain BCM-300.10 genes were differentially expressed in both mutated strains. Combining bioinformatic analysis and site-directed mutagenesis, we demonstrated that motifs overlapping the promoter influence the expression of genes directly, while methylation of other motifs might cause secondary effects.Thus, m5C methylation modifies the transcription of multiple genes, affecting important phenotypic traits that include adherence to host cells, natural competence for DNA uptake, bacterial cell shape, and susceptibility to copper.
Self-confidence and fear of failure among university students and their relationship with entrepreneurial orientation
Authors: Martins Izaias , Monsalve Juan Pablo Pérez , Martinez Andres Velásquez 
Published: August 2018

PurposeThe purpose of this paper is to identify the impact of features of personality such as self-confidence and fear of failure on the entrepreneurial orientation (EO) of university students enrolled on entrepreneurial education courses.Design/methodology/approachVariables related to risk-taking, innovativeness, proactiveness, as well as those related to self-confidence and fear of failure, are taken into account. Using linear regression, the authors investigate how self-confidence and fear of failure affect the EO of university students.FindingsAs pointed out by results, both self-confidence and fear of failure are determinants of the EO of university students. Self-confidence has a positive and consistent effect on the three dimensions of EO, whereas fear of failure has a negative effect on EO.Research limitations/implicationsIt is not possible to assure that, in the medium and long term, individuals more prone to taking risks, innovating and proactivity will in fact become entrepreneurs. Also, even though it does not affect the relevance of the findings, it must be highlighted that this study has been carried out with a specific sample of students and results may vary in different contexts.Originality/valueThis study offers a new insight relating individual’s self-perceptions and their impact on EO. Equally important, the findings of this paper offer relevant information for the design of academic programs aimed at strengthening students’ personal aspects to promote self-confidence and tolerance to fear of failure as predictors of the EO in this collective.
Embracing localization: evidence from Western MNEs in Ghana
Authors: Azungah Theophilus , Michailova Snejina , Hutchings Kate 
Published: August 2018

Purpose
Despite the growing economic importance of Africa, the region has received scant attention in the international human resource management literature. The purpose of this paper is to address the gap in examining human resource management (HRM) practices in Western multinational enterprises’ (MNEs) subsidiary operations in Ghana, which is a significant foreign direct investment market in Africa. Focusing on recruitment and selection, training and development, performance management, and rewards management viewed through the ability, motivation and opportunity (AMO) framework, the research emphasizes that effectiveness of the MNEs’ cross-cultural operations has necessitated embracing localization across a range of practices in accordance with the Ghanaian cultural landscape and specificities.

Design/methodology/approach
The paper draws on 37 in-depth interviews with managers and employees in eight subsidiaries of British, European and US MNEs in Ghana. Interviews were conducted in 2015 in three locations – the capital city Accra, Tema (in the south) and Tamale (in the north).

Findings
The research reinforces earlier literature emphasizing the importance of paternalism and family and to a lesser extent patronage, but presents new findings in highlighting the erstwhile unexplored role of local chiefs in influencing HRM practices in Western MNEs in Ghana. Utilizing the AMO framework, this paper highlights practices within each HR area that influences performance through impact on employee AMO.

Practical implications
The research informs MNE managers about the strategic importance of observing local cultural practices and designing appropriate strategies for ensuring both operational effectiveness and successful cross-cultural collaboration with local managers and employees in Ghana. It is suggested that if managers implement practices that foster and enhance employee AMO, subsidiaries may benefit from employee potential and discretionary judgment.

Originality/value
This paper contributes to a dearth of literature on HRM practices of Western MNEs’ subsidiaries in Africa by examining the extent to which MNEs strategically localize their practices to accommodate specificities of the host country cultural context and operate successfully.
Design of polyester-elastane-cotton plated knitted components for hot and dry environment
Authors: Ramakrishnan Varadaraju , Jagannathan Srinivasan 
Published: August 2018

PurposeThe purpose of this paper is to optimize the linear densities of polyester yarn and filament for inner layer and elastane for middle layer with cotton yarn outer layer in plain knitted plated structure for hot and dry environment clothing.Design/methodology/approachThree levels of polyester yarn linear densities (11.1, 8.4 and 5.6 Tex), filament linear densities (0.8, 1.55 and 2.3 Decitex) and elastane (0, 4 and 8 percent) with 14.75 Tex cotton yarn have been used to knit 15 single jersey plated fabrics based on Box and Benhens experimental design with same loop length. Three cotton–elastane core-spun fabrics were also produced. All the fabrics were analyzed for moisture and ergonomic comfort properties and wet fabric coefficient of friction.FindingsThe increase in elastane content and yarn linear density decreases water vapor and air permeability; the increase in filament linear density decreases wicking rate and water absorbency. The optimum solution is 5.55 Tex polyester yarn of 0.8 Decitex filament as inner layer and 14.75 Tex cotton yarn as outer layer which gives good heat and moisture transfer without stickiness.Research limitations/implicationsThe implication of this paper is to study thinner polyester, polypropylene and polyethylene fabrics with more micro pores as skin contact layer for quicker heat and moisture transfer.Practical implicationsOutward wickability of sweat from the skin is the prime requirement of all skin contact layer fabrics.Social implicationsIt shifts the social attitude of most comfortable fabric to polyester–cotton plated for hot and dry climate.Originality/valueThis paper employs a more practical method for the selection of fabric.
Double bind in loan access in China: the reification of gender differences in business loans
Authors: Xu Huacen (Brin) , Zhan Heying Jenny , James Claire Elizabeth-Ellen , Fannin Lauren Denise , Yin Yue 
Published: August 2018

PurposeThis paper aims to examine gender differences in credit access and credit default.Design/methodology/approachUsing panel data drawn from 917 valid credit borrowers covering the period 2012 to 2015 drawn from among 6,849 study subjects and a national household financial survey (n = 29,500) conducted in China, this study focuses on gender differences in small and micro entrepreneurs’ financial behavior, specifically with respect to credit access and credit default.FindingsThe study revealed the following: Women expressed having more barriers to obtaining a business loan than men; gender had a significant effect on women’ credit default; and women were less likely to default a loan than male loan borrowers did. An exploration of the reasons for credit access and default found that female loan applicants were more likely to display a lack of knowledge and confidence in loan application.Originality/valueThe study contributes to literature by using the Marxian concept of reification in explaining women and their financial behaviors in China.
Interactions in sustainable supply chain management: a framework review
Authors: Yun Gawon , Yalcin Mehmet G. , Hales Douglas N. , Kwon Hee Yoon 
Published: August 2018

PurposeThe purpose of this paper is to evaluate the research conducted among the interim, dyadic interactions that bridge the stand-alone measures of economic, environmental and social performance and the level of sustainability, as suggested in the Carter and Rogers (2008) framework.Design/methodology/approachThis paper conducts a systematic literature review based on the Tranfieldet al.(2003) method of the articles published in 13 major journals in the area of supply chain management between the years 2010 and 2016. Results were analyzed using an expert panel.FindingsThe area of research between environmental and social performance is sparse and relegated to empirical investigation. As an important area of interaction, this area needs more research to answer the how and why questions. The economic activity seems to be the persistent theme among the interactions.Research limitations/implicationsThe literature on the “environmental performance and social performance (ES)” interactions is lacking in both theoretical and analytical content. Studies explaining the motivations, optimal levels and context that drive these interactions are needed. The extant research portrays economic performance as if it cannot be sacrificed for social welfare. This approach is not in line with the progressive view of sustainable supply chain management (SSCM) but instead the binary view with an economic emphasis.Practical implicationsTo improve sustainability, organizations need the triple bottom line (TBL) framework that defines sustainability in isolation. However, they also need to understand how and why these interactions take place that drive sustainability in organizations.Originality/valueBy examining the literature specifically dedicated to the essential, interim, dyadic interactions, this study contributes to bridging the gap between stand-alone performance and the TBL that creates true sustainability. It also shows how the literature views the existence of sustainability is progressive, but many describe sustainability as binary. It is possible that economic sustainability is binary, and progressive characterizations of SSCM could be the reason behind the results favoring economic performance over environmental and social.