bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogeny of the plant bug subfamily Mirinae (Hemiptera: Heteroptera: Cimicomorpha: Miridae) based on total evidence analysis
Authors: Kim Junggon , Jung Sunghoon 
Published: March 2019

AbstractThe first comprehensive phylogenetic analyses of the most diverse subfamily of plant bugs, Mirinae, is presented in this study, for 110 representative taxa based on total evidence analysis. A total of 85 morphological characters and 3898 bp of mitochondrial (16S, COI) and nuclear (18S, 28S) sequences were analysed for each partitioned and combined dataset based on parsimony, maximum likelihood and Bayesian inference. Major results obtained in this study include monophyly of the tribe Mecistoscelini. The largest tribe, Mirini, was recovered as polyphyletic, and Stenodemini was recovered as paraphyletic. The clade of Stenodemini + Mecistoscelini is the sister group of the remaining Mirinae. The monophyly of two complexes composed of superficially similar genera were tested; the Lygus complex was recovered as nonmonophyletic, and the Adelphocoris–Creontiades–Megacoelum complex was confirmed to be monophyletic. The generic relationships of the main clades within each tribe based on the phylogeny, as well as their supported morphological characters, are discussed.
Post‐Ebola psychosocial experiences and coping mechanisms among Ebola survivors: a systematic review
Authors: James P. B. , Wardle J. , Steel A. , Adams J. 
Published: March 2019

AbstractObjectiveA myriad of physical and psychosocial sequelae have been reported among Ebola survivors from previous Ebola virus disease (EVD) outbreaks, including the most recent in West Africa. This review examines the various forms of psychological distress experienced by EVD survivors, family and community reactions to EVD survivors and EVD survivors’ coping mechanisms.MethodsWe conducted a literature search of original articles employing Medline (Ovid), PubMed, Web of Science, Scopus, CINAHL, EBSCO host academic search complete, PsycINFO (EBSCO) and Embase databases.ResultsOur search identified 1890 articles of which 24 met our inclusion criteria. Various forms of psychological distress were prevalent among EVD survivors including depression, anxiety, anger, grief, guilt, flashbacks, sadness, worthlessness, substance addiction, suicidal tendencies and self‐stigmatisation. Family and community responses to EVD survivors ranged from acceptance to rejection, isolation, stigmatisation and discrimination. EVD survivors’ coping strategies included engagement with religious faith, EVD survivors associations and involvement in EVD prevention and control interventions.ConclusionPsychological distress, including that resulting from family and community stigma and discrimination, appears common among EVD survivors. Community‐based mental health and psychosocial interventions integrated within a broader package of care for EVD survivors that also recognises the physical health challenges are required. Well‐designed longitudinal studies can provide clear insights on the nature and trajectory of the psychosocial issues currently experienced by EVD survivors.
The Arabidopsis pentatricopeptide repeat protein LPE1 and its maize ortholog are required for translation of the chloroplast psbJ RNA
Authors: Williams‐Carrier Rosalind , Brewster Carolyn , Belcher Susan E. , Rojas Margarita , Chotewutmontri Prakitchai , Ljungdahl Sonja , Barkan Alice 
Published: March 2019

SummaryThe expression of chloroplast genes relies on a host of nucleus‐encoded proteins. Identification of such proteins and elucidation of their functions are ongoing challenges. We used ribosome profiling to revisit the function of the pentatricopeptide repeat protein LPE1, reported to stimulate translation of the chloroplast psbA mRNA in Arabidopsis. Mutation of the maize LPE1 ortholog causes a photosystem II (PSII) deficiency and a defect in translation of the chloroplast psbJ open reading frame (ORF) but has no effect on psbA expression. To reflect this function, we named the maize LPE1 ortholog Translation of psbJ 1 (TPJ1). Arabidopsis lpe1 mutants likewise exhibit a loss of psbJ translation, and have, in addition, a decrease in psbN translation. We detected a small decrease in ribosome occupancy on the psbA mRNA in Arabidopsis lpe1 mutants, but ribosome profiling analyses of other PSII mutants (hcf107 and hcf173) in conjunction with in vitro RNA binding data strongly suggest that this is a secondary effect of their PSII deficiency. We conclude that maize TPJ1 promotes PSII synthesis by activating translation of the psbJ ORF, that this function is conserved in Arabidopsis LPE1, and that an additional role for LPE1 in psbN translation contributes to the PSII deficiency in lpe1 mutants.
Survival of human cadaver skin on severe combined immune deficiency pigs: Proof of concept
Authors: Singer Adam J. , Tuggle Christopher , Ahrens Amanda , Sauer Mary , McClain Steve A. , Tredget Edward , Rosenberg Lior 
Published: March 2019

AbstractTransplantation of human xenografts onto immunocompromised mice is a powerful research tool for studying wound healing. However, differences in healing between humans and mice and their small size limit this model. We determined whether human cadaver skin xenografts transplanted onto pigs with severe combined immune deficiency (SCID) would survive and not be rejected. Meshed (1:1.5), cryopreserved human cadaver skin was transplanted onto 10 partial thickness dermatome wounds in each of two normal domestic pigs and two SCID pigs. Autografts (n = 2/animal) from the four animals were used as controls. In normal pigs, all autografts were engrafted and healed with a minimal, if any, inflammation and scarring. All human xenografts were rejected by the normal pigs within 5–11 days and associated with an intense T‐cell inflammatory response. In contrast, both autografts and xenografts were engrafted and survived the 28‐day study in the SCID pigs with a minimal inflammation and no gross scarring.
Association of genetically predicted testosterone with thromboembolism, heart failure, and myocardial infarction: mendelian randomisation study in UK Biobank
Authors: Luo Shan , Au Yeung Shiu Lun , Zhao Jie V , Burgess Stephen , Schooling C Mary 
Published: March 2019

Abstract

Objective
To determine whether endogenous testosterone has a causal role in thromboembolism, heart failure, and myocardial infarction.

Design
Two sample mendelian randomisation study using genetic variants as instrumental variables, randomly allocated at conception, to infer causality as additional randomised evidence.

Setting
Reduction by Dutasteride of Prostate Cancer Events (REDUCE) randomised controlled trial, UK Biobank, and CARDIoGRAMplusC4D 1000 Genomes based genome wide association study.

Participants
3225 men of European ancestry aged 50-75 in REDUCE; 392 038 white British men and women aged 40-69 from the UK Biobank; and 171 875 participants of about 77% European descent, from CARDIoGRAMplusC4D 1000 Genomes based study for validation.

Main outcome measures
Thromboembolism, heart failure, and myocardial infarction based on self reports, hospital episodes, and death.

Results

Of the UK Biobank participants, 13 691 had thromboembolism (6208 men, 7483 women), 1688 had heart failure (1186, 502), and 12 882 had myocardial infarction (10 136, 2746). In men, endogenous testosterone genetically predicted by variants in the
JMJD1C
gene region was positively associated with thromboembolism (odds ratio per unit increase in log transformed testosterone (nmol/L) 2.09, 95% confidence interval 1.27 to 3.46) and heart failure (7.81, 2.56 to 23.8), but not myocardial infarction (1.17, 0.78 to 1.75). Associations were less obvious in women. In the validation study, genetically predicted testosterone (based on
JMJD1C
gene region variants) was positively associated with myocardial infarction (1.37, 1.03 to 1.82). No excess heterogeneity was observed among genetic variants in their associations with the outcomes. However, testosterone genetically predicted by potentially pleiotropic variants in the
SHBG
gene region had no association with the outcomes.

Conclusions
Endogenous testosterone was positively associated with thromboembolism, heart failure, and myocardial infarction in men. Rates of these conditions are higher in men than women. Endogenous testosterone can be controlled with existing treatments and could be a modifiable risk factor for thromboembolism and heart failure.
PD-L1 expression on routine samples of non-small cell lung cancer: results and critical issues from a 1-year experience of a centralised laboratory
Authors: Vigliar Elena , Malapelle Umberto , Iaccarino Antonino , Acanfora Gennaro , Pisapia Pasquale , Clery Eduardo , De Luca Caterina , Bellevicine Claudio , Troncone Giancarlo 
Published: March 2019

AimsOur laboratory is a centralised centre receiving routine non-small cell lung cancer (NSCLC) samples for programmed death ligand-1 (PD-L1) immunohistochemical (IHC) evaluation. Since literature data are not concordant here we review our clinical records to assess the rate of PD-L1 positive and negative NSCLC cases in real-world practice.MethodsPD-L1 expression was evaluated by a validated 22C3 IHC laboratory developed test on 211 prospectively collected routine NSCLC samples, received from 10 outside institutions. PD-L1 expression was assessed by the tumour proportion score (TPS) and reported by using a three cut-point system: TPS<1, TPS 1%–49% and TPS≥50%.ResultsOverall, 193 out of 211 samples (91.5%) meet the criteria for adequacy (more than 100 viable neoplastic cells). In 62.7% (121/193) of samples TPS was <1%; 17.6% of samples (34/193) expressed PD-L1 with a TPS of 1%–49% and 19.7% (38/193) with a TPS of >50%. There was no significant difference in PD-L1 expression rates between different histotypes and site of sampling. Instead, a statistically significant difference was associated to the type of samples: in fact, cytological samples were more frequently negative for PD-L1 expression (TPS<1%) and less often displayed PD-L1 expression at high levels (TPS>50%) than surgical resections and biopsies.ConclusionsWe present a referral laboratory experience on IHC PD-L1 expression of prospectively collected routine NSCLC samples. Data from the real-world practice can better clarify the percentage of PD-L1 positive and negative cases, to establish benchmarks for good practice standards.
A multi-parameter integrated chip system for water quality detection
Authors: Wang Jiaqi , Bian Chao , Li Yang , Sun Jizhou , Tong Jianhua , Xia Shanhong 
Published: March 2019

Water pollution has always been an important issue threatening human health. Water quality monitoring is an important step to ensure water quality safety. Water quality parameters such as pH, temperature and conductivity are important indicators in water quality monitoring. In this paper, a multi-parameter water quality detection integrated chip system is studied, and multiple sensors are integrated on the same chip to realize the monitoring of conventional parameters such as pH, temperature and conductivity in drinking water. The chip system was processed by MEMS technology. A ruthenium oxide (RuO2) film is used for pH detection, and the sensor exhibits a super-Nernst response (62.88 mV/pH) in the range of pH 1.92–11.74. The patterned micro Pt film is used as the temperature sensor, and the three-wire orthogonal structure is used for temperature detection. The sensitivity of this temperature sensor is 5.517 [Formula: see text]/[Formula: see text]C in the range of 6 to 80[Formula: see text]C. Conductivity was measured by a four-electrode system, and the electrode constant was 1.667 cm[Formula: see text] measured between 0.994 and 21.167 mS/cm. The test results show that the multi-parameter integrated chip system can realize the simultaneous detection of pH, temperature and conductivity in water, and has the characteristics of miniaturization and low cost, and provides an effective way for simultaneous detection of water quality.
Interaction between the folded structures of the Western Papua New Guinea Highlands: an example of how surface observations can assist in subsurface understanding
Authors: Ollarves Reinaldo , Zhao Siyuan , Gilby Fleur 
Published: March 2019

Abstract
We defined the folding sequence of the fold–thrust belt of the Western Papua New Guinea Highlands by analysing the surface expression of the structures and the response of the drainage system to the active fold–thrust belt. The interaction between structures is typically assessed by examining the syn-kinematic strata preserved; however, in our study area, this is problematic as these strata are poorly imaged on seismic lines. This study found common morphological features that allowed grouping and mapping of three different structural settings: group I, basement-involved tectonics; group II, thin-skinned anticlines which sole near the Koi–Iangi Sandstone; and group III, thin-skinned folds associated with the intra-Ieru Formation detachment. Fold Front Sinuosity analysis supports the idea that the fold–thrust belt propagates from the NW to the SE. Considering the detailed morphotectonics and drainage analyses, we interpret that the group II folds developed as out-of-sequence thrusting and folding, associated with buttressing against the group I larger structures.
Variation in syn-subduction sedimentation patterns from inner to outer portions of deep-water fold and thrust belts: examples from the Hikurangi subduction margin of New Zealand
Authors: McArthur A. D. , Claussmann B. , Bailleul J. , Clare A. , McCaffrey W. D. 
Published: March 2019

Abstract

The structure and distribution of accommodation in fold and thrust belts vary both laterally and longitudinally. Here we integrate gravity, bathymetry and 2D seismic datasets to investigate the structural and stratigraphic variation in the southern part of the Hikurangi subduction wedge, onshore and offshore North Island, New Zealand. Three morphostructural portions are recognized:

The inner portion demonstrates reactivation of inherited structures, producing thick-skinned deformation. Pre-subduction rocks are represented by kilometres of acoustically chaotic seismofacies. Thick-skinned deformation and readily deformable substrate lead to the development of wide trench-slope sub-basins, infilled with >5 km of syn-subduction sediments.

The mid portion typically demonstrates thrust faults with connections to deeper structures, leading to the development of an imbricate system with asymmetrical sub-basins typically <5 km thick developed on the back-limb of thrust related folds.

An antiformal stack marks the transition from the thick-skinned interior of the basin to the thin-skinned accretionary prism. Beyond this, the relatively non-deformed outer portion demonstrates frontal folds, propagating thrusts and up to 3 km thickness of syn-subduction strata.

Structural variation across the subduction wedge controls the generation of accommodation with implications for sediment distribution within fold and thrust belts and for petroleum system development.
Temporal Relational Ranking for Stock Prediction
Authors: Feng Fuli , He Xiangnan , Wang Xiang , Luo Cheng , Liu Yiqun , Chua Tat-Seng 
Published: March 2019

Stock prediction aims to predict the future trends of a stock in order to help investors make good investment decisions. Traditional solutions for stock prediction are based on time-series models. With the recent success of deep neural networks in modeling sequential data, deep learning has become a promising choice for stock prediction.However, most existing deep learning solutions are not optimized toward the target of investment, i.e., selecting the best stock with the highest expected revenue. Specifically, they typically formulate stock prediction as a classification (to predict stock trends) or a regression problem (to predict stock prices). More importantly, they largely treat the stocks as independent of each other. The valuable signal in the rich relations between stocks (or companies), such as two stocks are in the same sector and two companies have a supplier-customer relation, is not considered.In this work, we contribute a new deep learning solution, namedRelational Stock Ranking(RSR), for stock prediction. Our RSR method advances existing solutions in two major aspects: (1) tailoring the deep learning models for stock ranking, and (2) capturing the stock relations in a time-sensitive manner. The key novelty of our work is the proposal of a new component in neural network modeling, namedTemporal Graph Convolution, which jointly models the temporal evolution and relation network of stocks. To validate our method, we perform back-testing on the historical data of two stock markets, NYSE and NASDAQ. Extensive experiments demonstrate the superiority of our RSR method. It outperforms state-of-the-art stock prediction solutions achieving an average return ratio of 98% and 71% on NYSE and NASDAQ, respectively.
Antitumor effects of all-trans retinoic acid and its synergism with gemcitabine are associated with downregulation of p21-activated kinases in pancreatic cancer
Authors: Wang Kai , Baldwin Graham S. , Nikfarjam Mehrdad , He Hong 
Published: March 2019

Pancreatic ductal adenocarcinoma (PDA) is one of the most lethal malignancies worldwide. All-trans retinoic acid (ATRA) has been used as an antistromal agent in PDA, and its antitumor effect has also been reported in various kinds of cancer, including PDA. Inhibition of p21-activated kinases (PAKs) is associated with decreased tumor growth and increased gemcitabine sensitivity. The aim of this study was to evaluate the inhibitory effects of ATRA alone and in combination with gemcitabine on cell growth and migration of wild-type and gemcitabine-resistant PDA cells and the potential mechanism(s) involved. Human (MiaPaCa-2) and murine (TB33117) PDA cell lines were incubated in increasing concentrations of gemcitabine to establish resistant clones. Cell growth, clonogenicity, and migration/invasion were determined using a sulforhodamine B assay, a colony formation assay, and a Boyden chamber assay, respectively. Protein expression was measured by Western blotting. ATRA reduced cell proliferation, colony formation, and migration/invasion in both wild-type and gemcitabine-resistant cell lines. PAK1 expression was significantly increased in resistant cells. Cells treated with ATRA showed decreased expression of PAK1, PAK2, PAK4, and α-smooth muscle actin. The combination of ATRA and gemcitabine synergistically reduced cell growth in both wild-type and gemcitabine-resistant cell lines. Depletion of PAK1 enhanced ATRA sensitivity in MiaPaCa-2 cells. In conclusion, the antitumor effects of ATRA and its synergism with gemcitabine are associated with downregulation of PAKs.NEW & NOTEWORTHY The inhibitory effect of all-trans retinoic acid (ATRA) on cell proliferation, colony formation, and migration/invasion was associated with downregulation of p21-activated kinases (PAKs), and depletion of PAK1 enhanced ATRA sensitivity in MiaPaCa-2 cells. The combination of ATRA and gemcitabine synergistically reduced cell growth in both wild-type and gemcitabine-resistant pancreatic ductal adenocarcinoma cells. As an important prognostic marker, α-smooth muscle actin also can be downregulated by ATRA in pancreatic ductal adenocarcinoma cells.
Plasma free fatty acid concentration is closely tied to whole body peak fat oxidation rate during repeated exercise
Authors: Frandsen Jacob , Vest Stine Dahl , Ritz Christian , Larsen Steen , Dela Flemming , Helge Jørn W. 
Published: March 2019

Plasma free fatty acids (FFA) are a major contributor to whole body fat oxidation during exercise. However, the extent to which manipulating plasma FFA concentrations will influence whole body peak fat oxidation rate (PFO) during exercise remains elusive. In this study we aimed to increase plasma FFA concentrations through a combination of fasting and repeated exercise bouts. We hypothesized that an increase in plasma FFA concentration would increase PFO in a dose-dependent manner. Ten healthy young (31 ± 6 yr) (mean ± SD) well-trained (maximal oxygen uptake 65.9 ± 6.1 ml·min−1·kg−1) men performed four graded exercise tests (GXTs) on 1 day. The GXTs were interspersed by 4 h of bed rest. This was conducted either in a fasted state or with the consumption of a standardized carbohydrate-rich meal 3.5 h before each GXT. Fasting and previous GXTs resulted in a gradual increase in PFO from 0.63 ± 0.18 g/min after an overnight fast (10 h) to 0.93 ± 0.17 g/min after ∼22 h of fasting and three previous GXTs. This increase in PFO coincided with an increase in plasma FFA concentrations ( r2 = 0.73, P < 0.0001). Ingestion of a carbohydrate-rich meal 3.5 h before each GXT resulted in unaltered PFO. This was also reflected in unchanged plasma FFA, glucose, and insulin concentrations. In this study we show that plasma FFA availability is closely tied to whole body PFO and that the length of fasting combined with previous exercise are robust stimuli toward increasing plasma FFA concentration, highlighting the importance for preexercise standardization when conducting GXTs measuring substrate oxidation. NEW & NOTEWORTHY We show that peak fat oxidation is increased in close relationship with plasma free fatty acid availability after combined fasting and repeated incremental exercise tests in healthy highly trained men. Therefore it may be argued that whole body fat oxidation rate measured in most cases after an overnight fast indeed does not represent whole body maximal fat oxidation rate but a whole body peak fat oxidation rate within the context of the preexercise standardization obtained in the study design.