bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laboratory management of Crimean-Congo haemorrhagic fever virus infections: perspectives from two European networks
Authors: Bartolini Barbara , Gruber Cesare EM , Koopmans Marion , Avšič Tatjana , Bino Sylvia , Christova Iva , Grunow Roland , Hewson Roger , Korukluoglu Gulay , Lemos Cinthia Menel , Mirazimi Ali , Papa Anna , Sanchez-Seco Maria Paz , Sauer Aisha V. , Zeller Hervè , Nisii Carla , Capobianchi Maria Rosaria , Ippolito Giuseppe , Reusken Chantal B. , Di Caro Antonino 
Published: January 2019

BackgroundCrimean-Congo haemorrhagic fever virus (CCHFV) is considered an emerging infectious disease threat in the European Union. Since 2000, the incidence and geographic range of confirmed CCHF cases have markedly increased, following changes in the distribution of its main vector,Hyalommaticks.AimsTo review scientific literature and collect experts’ opinion to analyse relevant aspects of the laboratory management of human CCHF cases and any exposed contacts, as well as identify areas for advancement of international collaborative preparedness and laboratory response plans.MethodsWe conducted a literature review on CCHF molecular diagnostics through an online search. Further, we obtained expert opinions on the key laboratory aspects of CCHF diagnosis. Consulted experts were members of two European projects, EMERGE (Efficient response to highly dangerous and emerging pathogens at EU level) and EVD-LabNet (Emerging Viral Diseases-Expert Laboratory Network).ResultsConsensus was reached on relevant and controversial aspects of CCHF disease with implications for laboratory management of human CCHF cases, including biosafety, diagnostic algorithm and advice to improve lab capabilities. Knowledge on the diffusion of CCHF can be obtained by promoting syndromic approach to infectious diseases diagnosis and by including CCHFV infection in the diagnostic algorithm of severe fevers of unknown origin.ConclusionNo effective vaccine and/or therapeutics are available at present so outbreak response relies on rapid identification and appropriate infection control measures. Frontline hospitals and reference laboratories have a crucial role in the response to a CCHF outbreak, which should integrate laboratory, clinical and public health responses.
Lyric Text Mining Of Dangdut: Visualizing The Selected Words And Word Pairs Of The Legendary Rhoma Irama’s Dangdut Song In The 1970s Era
Authors: Fahrudin Tresna Maulana , Barakbah Ali Ridho 
Published: January 2019

Dangdut is a new genre of music introduced by Rhoma Irama, Indonesian popular musician who was the Legendary dangdut singer in the 1970s era until now. The expression of Rhoma Irama’s lyric has themes of the human being, the way of life, love, law and human right, tradition, social equality, and Islamic messages. But interestingly, the song lyrics were written by Rhoma Irama in the 1970s were mostly on the love song themes. In order to prove this, it is necessary to identify the songs through several approaches to explore the selected word and the relationship between word pairs. If each Rhoma Irama’s lyric is identified in text mining field, the lyric text extraction will be an interesting knowledge pattern. We collected the lyric from web were used as datasets, and then we have done the data extraction to store the component of lyric including the part and line of the song. We successfully applied the most word frequencies in the form of data visualization including bar chart, word cloud, term frequency-inverse document frequency, and network graph. As a results, several word pairs that often was used by Rhoma Irama in writing his song including heart-love (19 lines), heart-longing (13 lines), heart-beloved (12 lines), love-beloved (12 lines), love-longing (11 lines).
Antioksidan KUMARIN TERISOPRENILASI DAN ALKALOID INDOL DARI KULIT BATANG Zanthoxylum ovalifolium TUTCHER
Authors: Tanjung Mulyadi 
Published: January 2019

Two isoprenylated coumarins, imperatorin (1), isopimpinelin (2) and one indol alkaloid compound, canthine-6-one (3) were isolated from the stem bark of Zanthoxylum ovalifolium Tutcher. Their structures were determined based on spectroscopic data such as UV, IR, MS and NMR. Imperatorin (1) showed high activity toward DPPH radical and isopimpinelin (2) and chantine-6-one (3) has moderate activity.
PENGARUH LAJU ALIR DALAM PROSES PENGEMASAN KOLOM KROMATOGRAFI PENUKAR ION
Authors: Simanjuntak Vino Soaduon Hamonangan 
Published: January 2019

Resin DEAE Separose Fast Flow adalah salah satu resin yang digunakan dalam kromatografi penukar ion. Dalam aplikasi resin diinstruksikan penggunaan konstan laju alir dalam proses packing kolom sangat terbatas yaitu dengan ketentuan bed height (tinggi resin dalam kolom) 15 cm. Di sisi lain, pengguna resin tidak selalu menggunakan jumlah resin yang sama banyak dan jenis kolom yang berbeda. Sehingga, proses kemas pada kolom gelas kaca (SAC 100/500 mm) maka, diperlukan studi lebih lanjut untuk menentukan tinggi kolom. Penelitian ini menggunakan laju alir yang direkomendasikan dari pemasok dan juga menggunakan laju alir yang berada di bawah rekomendasi pemasok, karena semakin tinggi laju alir membuat hasil kemas pada kolom tidak efisien. Hasil penelitian menunjukkan bahwa laju alir yang baik adalah 150 mL/menit yang memberikan nilai asimetri 1,8 dan nilai HETP (angka lempeng teoritis) yang memenuhi criteria dibanding dengan menggunakan laju alir yang lainnya.
Taxonomic composition and microecological indices of skin and subcutaneous tissue purulent-inflammatory processes
Authors: R. I. Sydorchuk  , O. Y. Khomko  , R. P. Knut  , P. M. Volyanyuk  , L. I. Sydorchuk  , O. M. Plehutsa  , S. I. Railanu  , I. Y. Sydorchuk  
Published: January 2019

The aim — to study the taxonomic composition and microecological indicators of causative agents of acute purulent‑inflammatory diseases of the skin and subcutaneous tissue.Materials and methods. A total of 97 patients with acute purulent‑inflammatory diseases of skin and subcutaneous tissue were examined, in 50 (51.55 %) patients were diagnosed boils, 47 (48.45 %) had chronic ulcers, hidradenitis, carbuncles, and common furunculosis. Intraoperatively or during the redressing, the sample was taken and a bacteriological examination was performed within 2 hours.Results and discussion. 197 strains of conditionally pathogenic gram‑positive (89.85 %) and gram‑negative (20 — 10.15 %) bacteria were investigated and identified in patients. The coagulase‑positive S. aureus is the main microbial group in the constancy index, frequency of occurrence, Margalef species richness index, Simpson species diversity index and Berger‑Parker species dominance.Conclusions. Acute purulent‑inflammatory diseases of the skin and subcutaneous tissue are polyetiological diseases that are caused by Gram‑positive and Gram‑negative, facultative anaerobic and aerobic bacteria with a significant predominance of Gram‑positive cocci in association with other conditionally pathogenic microorganisms. In terms of taxonomic composition, microecological indicators, the leading causative agents of acute purulent‑inflammatory diseases of the skin and subcutaneous tissue are S. aureus, P. aeruginosa, S. pyogenes and P. vulgaris, E. coli, E coli Hly+, and E. aerogenes (1.05 %).
Estimation of Cutoff Values by Using Regression Lines Method in Mishrif Reservoir/ Missan oil Fields
Authors: Al Jawad Mohammed Salih , Tariq Baraa Ziad 
Published: January 2019

Net pay is one of the most important parameters used in determining initial oil in place of a reservoir. It can be delineated through the using of limiting values of the petrophysical properties of the reservoir. Those limiting values are named as the cutoff. This paper provides an insight into the application of regression line method in estimating porosity, clay volume and water saturation cutoff values in Mishrif reservoir/ Missan oil fields. The study included 29 wells distributed in seven oilfields of Halfaya, Buzurgan, Dujaila, Noor, Fauqi, Amara and Kumait.
This study is carried out by applying two types of linear regressions: Least square and Reduce Major Axis Regression.
The Mishrif formation was divided into three main units. They are MA, MB, and MC. The methods were applied to each unit of Mishrif formation individually and as one unit. The division of Mishrif formation into subunits led to a great improvement in the accuracy of the porosity-permeability correlations. The Results indicated that the regression lines method of defining cutoffs gives unrealistic values with the common assumption of permeability cutoff = 0.1 md.
Another assumption for permeability cutoff = 1 is made and it was chosen due to lithology and hydrocarbon type which are limestone and oil respectively. This assumption led to more realistic and higher porosity cutoff and smaller water saturation and clay volume cutoff values using the two types of regression lines.
Surgical site infection in Laparoscopic versus Open appendicectomy
Authors: Koirala Ashok , Thakur Dipendra , Agrawal Sunit , Chaudhary Bhuwan Lal , Poudel Sagar 
Published: January 2019

Background: Acute appendicitis is very common surgical cause of acute abdomen and needs surgical removal either by laparoscopic or open appendicectomy. The aim of this study is to compare frequency of surgical site infection (SSI) in patients undergoing laparoscopic and open appendicectomy.
Materials and Methods: The study was prospective study conducted in NMCTH, Biratnagar. Total 200 patients with diagnosis of acute appendicitis admitted through the emergency department of our hospital were included in the study. The patients were randomly allocated in two groups: Laparoscopic appendicectomy group (LA) and Open appendicectomy group (OA). Both groups underwent successful emergency appendicectomy. Wound infections in terms of surgical site infection (SSI) if present were recorded. All age groups and both sexes were included.
Results: Two hundred patients underwent appendicectomy, one hundred Laparoscopic appendicectomy (LA) and another hundred open appendicectomy (OA). The mean age of patients with acute appendicitis was 30.63±16.14 years with minimum of 6 years and maximum of 77 years. The highest number of patients were in age group of 10 to 20 years (29.5%). In LA group SSI noted in 3 patients (3%) whereas in OA group it was found in 12 patients (12%).
Conclusion: Laparoscopic appendicectomy is better and offers great advantages in terms of SSI as compared to Open appendicectomy.
Effects of curcumin on testis microvascular complication in streptozotocin-induced diabetes rats
Authors: Khimmaktong Wipapan , Komolkriengkrai Manaras , Boonyoung Piyakorn , Anupunpisit Vipavee 
Published: January 2019

Background: Diabetes has a significant impact on the impaired function of the reproductive system in the testis.Objective: The aim of this study was to investigate the effects of curcumin from turmeric on structural changes of testicular tissues and microvasculature in STZ-induced diabetic rats.Methods: Rats were injected with a single dose of streptozotocin (60 mg/kg BW) to induce a diabetic condition. Three days after the injection, rats with a blood sugar level > 250 mg/dl were considered diabetic. Rats were allocated to one of three groups, diabetic (DM), diabetic rats receiving curcumin (DMC) (200 mg/kg BW), and control (C). Testicular tissues were studied eight weeks after the treatment using the histological technique. Testicular microvasculature was examined using the vascular corrosion cast technique under a scanning electron microscope (SEM).Results: The testicular tissue of DM group showed damaged seminiferous tubules, spermatocytes, and spermatids with necrosis and germ cells degeneration. Destruction of testicular arteries and veins in pampiniform plexus were demonstrated. The destroyed capillaries, arterioles, and venules demonstrated stenosis and shrinkage. The vessel diameter in all DM groups decreased. In the DMC group which received curcumin, the appearance of all seminiferous tubules, spermatogenic, and sertoli cell was normal. The reconstruction and restoration of testicular artery, pampiniform plexus, capillary network, arterioles, and venules were clear. As a result of curcumin supplementation, the standard and healthy testis microvasculature was restored. The DMC vessels diameter increased back to a quasi normal condition, particularly at the artery, arteriole, and capillary levels.Conclusions: The results provided evident for effects of curcumin could be associated with recovering and improvement of the testicular tissues and microvasculatures in diabetic condition.Keywords: Diabetes mellitus, Streptozotocin, Curcumin, Testis, Microvasculature, Vascular corrosion cast, Scanning electron microscope
The anti-inflammatory effects of Indonesian and Japanese bitter melon (Momordica charantia L.) fruit extracts on interleukin-1β-treated hepatocytes
Authors: Dwijayanti Dinia Rizqi , Okuyama Tetsuya , Okumura Tadayoshi , Ikeya Yukinobu , Nishizawa Mikio 
Published: January 2019

Background: Bitter melon, Momordica charantia L. (Cucurbitaceae), grows in tropical to temperate regions in Asia, including Indonesia and Japan. Bitter melon contains triterpenoids, steroidal glycosides, flavonoids, and alkaloids. The green immature fruit of bitter melon is a popular vegetable in Indonesia and Japan and has been used as a traditional Indonesian medicine (Jamu) to treat microbial infections and diabetes mellitus. However, there are currently few reports about the pharmacological effects of the constituents of bitter melon.Objective: To compare the effects of Indonesian and Japanese bitter melon, the production of pro-inflammatory mediators, such as nitric oxide (NO) and pro-inflammatory cytokines, was monitored.Methods: Immature fruits of bitter melons collected from Indonesia and Japan were lyophilized and extracted with methanol. The resultant extracts were fractionated by hydrophobicity into ethyl acetate-soluble (A), n-butanol-soluble (B), and water-soluble (C) fractions. Primary cultured hepatocytes prepared from rat liver were treated with interleukin-1β (IL-1β) and each extract or fraction. Nitrite in the medium was measured, and the half-maximal inhibitory concentration (IC50) value was determined. Lactate dehydrogenase activity in the medium was measured to monitor cytotoxicity. The expression of inducible nitric oxide synthase (iNOS) was detected by western blot analysis. The mRNA levels were measured by reverse transcription-polymerase chain reaction. Results: Indonesian and Japanese bitter melon extracts (BMEs) suppressed NO production by IL-1β-treated hepatocytes without showing cytotoxicity. The IC50 values of the BMEs were 86 μg/mL (Indonesian) and 171 μg/mL (Japanese). Both BMEs suppressed iNOS protein expression. Fractions A and B of both BMEs suppressed NO production. The IC50 values of the Indonesian BME fractions A and B were comparable to those of Japanese BME. Fraction A of both BMEs decreased the levels of iNOS protein and mRNA, as well as those of pro-inflammatory cytokine mRNAs. Fraction C of both BMEs did not affect NO production.Conclusion: The ethyl acetate-soluble fraction (A) of Indonesian and Japanese BMEs exhibited a comparable ability to suppress the induction of the iNOS and pro-inflammatory cytokine genes. These data suggest that hydrophobic constituents in fraction A, such as cucurbitane-type triterpenoids, may be responsible for the observed anti-inflammatory effects of bitter melon from Indonesia and Japan.Keywords: Momordica charantia, nitric oxide, inflammation, Jamu, Goya.
Toksisitas moluskisida fentin asetat terhadap hematologi dan pertumbuhan ikan nila, Oreochromiss niloticus (Linnaeus, 1758)
Authors: Lukmini Aisyah , Supriyono Eddy , Budiardi Tatag 
Published: January 2019

Fentin acetate (C20H18O2Sn) as pesticide is extensively used for killing golden apple snail (Pomacea sp.) in paddy field. This study was aimed to determine effect of sublethal molluscicide fentin acetate toxicity on the haematological charac-teristics (erythrocyte, hemoglobin, hematocrit, and leucocyte) and growth of juvenile Oreochromis niloticus. This re-search was conducted from May to July 2015 in Environment Laboratory of Aquaculture Department, Bogor Agricul-tural University. The research used twelve glass aquariums of 100x50x50 cm3 filled with 160 L water and put 30 juve-niles per aquarium. Fish were fed at satiation during the treatment and water exchange for every 24 hour. Research design was complete experimental randomized with four treatments and three replications of different fentin acetate concentrations i.e. 0.00; 0.003; 0.008; 0.015 mg L-1 for 21 days. The haematological characteristics and growth of fish were compared with ANOVA. The result indicates that sublethal concentration of 0.003 mg.L-1fentin acetate was significantly (p< 0.05) decrease the haematological characteristics and growth of tilapia. AbstrakFentin asetat (C20H18O2Sn) digunakan sebagai pestisida di sawah secara intensif untuk mematikan keong mas (Pomacea sp.). Tujuan penelitian ini adalah untuk menentukan pengaruh toksisitas subletal moluskisida fentin asetat terhadap ka-rakteristik hematologi (eritrosit, hemoglobin, hematokrit, dan leukosit) dan pertumbuhan yuwana ikan nila (Oreo-chromis niloticus). Penelitian dilaksanakan pada bulan Mei sampai Juli 2015 di Laboratorium Lingkungan Departemen Akuakultur, Institut Pertanian Bogor. Penelitian ini menggunakan 12 akuarium berukuran 100x50x50 cm3. Ikan nila berukuran 8,90±0,13 g dipelihara dengan kepadatan 30 ekor dalam volume air 160 L. Ikan uji diberi pakan secara at satiation serta dilakukan penggantian air setiap 24 jam dengan konsentrasi bahan uji yang sama. Rancangan penelitian yang digunakan adalah rancangan acak lengkap dengan empat perlakuan dan tiga ulangan dengan konsentrasi fentin asetat, yaitu: 0,00 (kontrol); 0,003; 0,008; dan 0,015 mg.L-1 selama 21 hari. Analisis terhadap karakteristik hematologi dan pertumbuhan ikan nila menggunakan anova. Konsentrasi sublethal moluskisida fentin asetat berpengaruh nyata terhadap penurunan karakteristik hematologi dan pertumbuhan ikan nila.
Struktur komunitas sumber daya ikan demersal berdasarkan kedalaman perairan di Laut Cina Selatan (WPP-NRI 711) [Community structure of demersal fish resources based on the depth of the waters in the South China Sea (Indonesia Fisheries Management Zone 711)]
Authors: Angin Robet Perangin , Sulistiono NFN , Kurnia Rahmat , Fahrudin Achmad , Suman Ali 
Published: January 2019

Information on distribution and community structure of demersal fish resources are important to be known as an input to the management of demersal fisheries. This study aimed to analyze the diversity and distribution of demersal fish resources based on the differences in the depth of the waters and the linkages to the environment. Research conducted in the South China Sea in May to June 2015 by operating a trawl gear in the station preset. The method of analysis of demersal fish diversity use some ecological indices i.e Margalef species richness index, Shannon-Wiener diversity index, Pielou evenness index, and Simpson dominance index. The ecological index value then associated with environmental conditions, using principal component analysis. Distribution of the ecological index indicated the stability of communities demersal fish resources getting better with the increase of depth. The most affected to the level of species richness and distribution of demersal fish were the parameters of depth, temperature and salinity, while the abundance distribution of fishes were associated with dissolved oxygen, and water transparency. The implication, that the water environmental conditions greatly affected the distribution and abundance of demersal fish. AbstrakInformasi mengenai persebaran dan struktur komunitas sumber daya ikan demersal penting sebagai bahan masukan untuk pengelolaan perikanan demersal. Penelitian ini bertujuan untuk menganalisis tingkat keanekaragaman dan persebaran sumber daya ikan demersal berdasarkan perbedaan kedalaman perairan, serta keterkaitannya dengan lingkungan. Penelitian dilaksanakan di Laut Cina Selatan pada bulan Mei sampai Juni 2015 dengan mengoperasikan alat tangkap pukat ikan di stasiun yang telah ditetapkan. Metode analisis keanekaragaman hayati ikan demersal menggunakan beberapa indeks ekologi yaitu indeks kekayaan jenis Margalef, indeks keanekaragaman Shannon-Wiener, indeks keseragaman Pielou, dan indeks dominansi Simpson. Nilai indeks ekologi tersebut kemudian dikaitkan dengan kondisi lingkungan, menggunakan analisis komponen utama. Hasil penelitian menunjukkan tingkat kestabilan komunitas sumber daya ikan demersal semakin baik seiring dengan meningkatnya kedalaman. Kedalaman, suhu, dan salinitas merupakan parameter yang paling memengaruhi tingkat kekayaan jenis serta persebaran sumber daya ikan demersal, sedangkan persebaran kelimpahan ikan sangat terkait dengan oksigen terlarut dan kecerahan perairan. Implikasinya, kondisi lingkungan perairan sangat memengaruhi persebaran dan kelimpahan ikan demersal.
Pengangkutan juvenil ikan gabus Channa striata (Bloch 1793) dengan kepadatan berbeda pada media bersalinitas 3 ppt [Transportation of juvenile striped snakehead (Bloch 1793) with different densities in 3 ppt salinity media]
Authors: Nasmi Jannesa , Nirmala Kukuh , Affandi Ridwan 
Published: January 2019

The present study aimed to ensure the effect 3 ppt of salt addition into the transportation media water to the water quality, suvival rate, daily growth rate, and stress level after transportation. This study was consisted of two phase, the first phase was fish transportation for 24 hours and the second phase was 21 days rearing after-transportation. Snake-head larvae’s transported with five treatments i.e a transportation media with no salt addition and stocked with 30 larvae L-1 as the control, and four transportation media with 3 ppt salt addition at different densities (30, 45, 60, and 75 larvae L-1). During the transportation, the water quality and survival rate were determined. The first phase experiment showed that salt addition in transportation media maintained the water quality and higher survival rate than control. After 24 hours, 30 larvae of each treatment were stocked into tank with 0 ppt salinity media. The results showed no significant differences in the survival rate and growth rate at the different densities without salt addition. The pH and blood glucose levels were slowly back to normal in day 21. AbstrakTujuan penelitian ini adalah untuk menganalisis pengaruh pemberian garam 3 ppt dalam media pengangkutan terhadap perubahan kualitas air dan laju sintasan selama pengangkutan, laju pertumbuhan harian, glukosa darah, dan pH darah pascapengangkutan. Penelitian ini terdiri atas dua tahap kegiatan, yaitu tahap satu adalah pengangkutan selama 24 jam dan tahap dua adalah pemeliharaan pascapengangkutan selama 21 hari. Pada tahap satu kantong plastik diisi air 1 L dan dilakukan pengepakan sesuai dengan perlakuan, yaitu perlakuan kontrol tanpa garam (kepadatan 30 ekor L-1) dan perla-kuan penambahan garam 3 ppt (kepadatan 30, 45, 60, dan 75 ekor L"1). Penelitian ini menggunakan metode rancangan acak lengkap dengan lima perlakuan dan empat ulangan. Hasil penelitian menunjukkan perlakuan dengan penambahan garam dalam media pengangkutan dapat mempertahankan kondisi kualitas air dan menghasilkan laju sintasan yang le-bih tinggi dibandingkan pada perlakuan tanpa garam. Tahap dua adalah pemeliharaan pascapengangkutan selama 21 hari. Setelah masa pengangkutan juvenil dari setiap perlakuan dan ulangan dipelihara 30 ekor per akuarium pada media bersalinitas 0 ppt. Hasil penelitian menunjukkan laju sintasan dan laju pertumbuhan pascapengangkutan tidak berbeda nyata pada setiap perlakuan. Glukosa darah dan pH darah perlahan kembali normal hingga pemeliharaan hari ke-21.