bluuebunny/crossref_metadata_2025_split huggingface dataset:
No evidence of association between apo E polymorphism and BMI and lipids profile in Turkmen population
Authors: Ahani Dr , S Eshghinia , NM Samaei , N Mamizade 
Published: August 2018

Apolipoprotein E (apoE) is a glycoprotein that plays an essential role in lipid transport and metabolism.The present study purposed to determine the distribution of apoE alleles in a Turkman population and its association with lipid profile. In a cross-sectional study 354 healthy Turkman were recruited. Fasting blood samples were collected for biochemical tests and DNA extracting. Genomic DNA was amplified using PCR-RFLP method. The resulting fragments of PCR product digestion were detected byÂ polyacrylamide gel electrophoresis. This study showed that Îµ3 allele and E3/E3 genotype are the most common allele and genotype, while Îµ2 and Îµ4 alleles had lower frequencies, respectively. Two homozygote genotypes E2E2 and E4E4 were not detected in our sample. Results didnâ€™t confirm any association between apo E polymorphism with lipids concentration in Turkmen population.Â
Biogenic synthesis of Iron oxide nanoparticles via optimization of nitrate reductase Enzyme using statistical experimental design
Authors: Zaki Sahar 
Published: August 2018

Statistically designed experiments were proceeded to evaluate nutritional and environmental parameters that affect nitrate reductase enzyme (NR) activity in Achromobacter sp. KT735046. Plackett-Burman design was performed for screening and identifying efficiently the significance of 15 culture conditions influencing NR activity. FeCl3Â·6H2O, Na2MoO4Â·2H2O and CuSO4.5H2O were the most significant variables positively influencing, whereas pH was the most significant negative contributors. The optimal levels of significant factors were further predicted from five level factorial designs, Central Composite Design (CCD). The optimum parameter values were CuSO4.5H2O (60.375) mg/l, FeCl3Â·6H2O (240 mg /L), pH (6.135) and Na2MoO4Â·2H2O (150 mg /L). The biogenic Iron oxide nanoparticles "IONPs" were characterized; the dark brown IONPs exhibit maximum absorption from 400 to 464 nm, XRD reveals crystallite rhombohedral hematite, EDX confirms presence of 62% of iron, TEM illustrates formation of tiny IONPs, 1.4 and 2.8 nm in size, Î¾ potential and PDI recorded -42.9 mV and 0.268 respectively exhibiting high stability and monodispersed with no agglomeration by the help of biomolecules. This study conclude that Achromobacter sp. KT735046 consider being biofactory for synthesis nanoparticles by the help of NR enzyme andÂ it may be the first time to optimize Achromobacter sp. KT735046 for IONPs biosynthesis using statistical designs.Â
Involvement of the Selenium Level in Plasma Glutathione Peroxidase Activity in Newly Diagnosed Patients with Gravesâ€™ Disease and Hashimoto Thyroiditis
Authors: MSEDDI Malek , GARGOURI Bochra , MNIF Fatma , ABID Mohamed , GUERMAZI Fadhel , ATTIA Hamadi , LASSOUED Saloua 
Published: August 2018

Disturbed antioxidant enzymes activities was known to be associated to Auto-Immune Thyroid Diseases (AITD), but it is noteworthy that the selenoenzyme glutathione peroxidase (GPx) showed the lowest activity and a selenium (Se) deficiency may be the major cause.Â Most studies conducted on AITD are focused on the effect of the Se supplementation on the evolution of these pathologies. The aim of this study was to evaluate the Se level in plasma of Gravesâ€™ disease (GD) and Hashimoto Thyroiditis (HT) patients and its relationship with the redox status of these AITD. For this purpose, the Se levels as well as the GPx activity were evaluated in the plasmas of 33 patients with AITD. Results were compared with those of 27 healthy controls and among the two groups of patients. Results concerning GD and HT patients revealed lower Se levels by comparison with healthy controls (p<0.01 and p<0.05 respectively). Just like the Se, the GPx activity was found to be significantly lower in GD and HT patients compared to controls (p<0.01, p<0.001 respectively). The comparison between AITD groups showed higher Se level for the HT group (p<0.05), whereas, no significant differences were noted for the GPx activity. The correlation study showed positive correlation between Se level and GPx activity in HT group (r=0.64, p<0.01); and negative correlations between Se and anti-thyroglobuline and anti-Thyroid Stimulating Hormone Receptor levels in GD group (r= -0.71, p<0.05 and r= -0.73, p<0.05 respectively). As a conclusion, our findings have shown that the Se deficiency may be involved in the redox misbalance in HT; further investigations are mandatory to elucidate the origin of the reduced GPx activity in GD patients.
Study genetic diversity in maize inbreds and improved hybrid vigor in hybrids produce to determine commercial hybrids in Iraq
Authors: Abed Ziyad  A. 
Published: August 2018

Â A field experiments was conducted in greenhouse to determinate the genetic diversity among 7 genotypes from maize(4 inbreds and 3hybrids) by using molecular markers with Random Amplified polymorphic DNA(RAPD),that shown high level of polymorphism among genotypes of maize ,where the percentage of polymorphism ranged from(66%) and (83.33%) the highest number of polymorphism band (16) and size fragment ranged between (3800 bp) with the primer ( Bnlg 1185 ) and the lowest 180 with the primer( Bnlg 1464).The genetic distance value ranged between (0.3451) and (0.6534) ,where the lowest genic distance between (k1 and k2),while the highest genetic distance between(k4) and (k3xk4).In this study RAPD markers were shown to be powerful to detect genetic diversity and provided us high polymorphism values within genotypes of maize ,also we can conclude for useful those primers for genetic studies in plant breeding programs for developing synthetic cultivars or improved inbreds of maize.Â
Comparative Proteomics Study of Outer Membrane Proteins from three Pathogenic Leptospira Species used in Vaccine
Authors: Aghaiypour Kolyani Khosrow , Shokri Rahman 
Published: August 2018

Â Background and Objective: Outer Membrane Proteins (OMPs) have an important role in pathogenecity and immunogenecity of Leptospira introgans. The aim of this study was chemical and immunological analysis of OMP extracted from three vaccinal strains of pathogenic Leptospira (L. canicola, L. grippotyphosa & L. sejroae hardjo) by electrophoresis and western blotting.Materials and Methods: Outer membrane enriched fractions that are insoluble in sodium N- lauryl sarcosinate isolated and studied by Sodium dodecyl sulfate â€“ polyacrylamide gel electrophoresis. For identifying of antigenic properties of different proteins in three strains, antiserums developed in rabbit against the whole three valent vaccine and also Â specific OMPs rom each of three Leptospira strains. The antiserum was used for immunological studies with immuno double diffusion and western blotting.Results: in SDS-PAGE five common protein bands with approximate molecular masses of 75,36,25,23 and 19 KDa were identified. After western blot studies identified four or five immunogenic band. In general, antiserum against L. grippotyphosa OMP has the highest ability in detecting common bands between three strains.Conclusion: Chemical and immunological comparisons between OMPs of the three strains which are being used in the commercial vaccine, provided useful documents to assess and maybe improve vaccine quality. Â It indicated that OMPs are one of the major Leptospira component contributing in immunity and protectivity. Comparison between individual OMPs of each serotype revealed that L. grippotyphosa ones could contribute more in vaccine induced protectively in comparison with the other two serotypes.
BIOSYNTHESIS OF pH RESPONSIVE SHAPE MEMORY HYDROGEL AND ITS BIOMEDICAL POTENTIAL
Authors: GUMEL AHMAD MOHAMMED 
Published: August 2018

Multifunctional hydrogels combining the capabilities of cellular pH responsiveness and shape memory, are highly promising for the realization of smart membrane filters, controlled drug released devices, and functional tissue-engineering scaffolds. Free radical biocatalytic polymerization catalyzed by immobilized Candida antarctica lipase B was used to fabricate the pH-responsive and shape memory hydrogel using medium-chain-length poly-3-hydroxyalkanoates-co-polyethylene glycol methacrylate (PHA-PEGMA) as macromer. The accelerative wound healing potential of the biosynthesized smart PHA based hydrogel was evaluated herein. The thermal stability of the macromere highly depends on the PEGMA fraction from 10 to 50% (mass). Similarly, the change in PEGMA fraction was also found to highly influence the hydrogelâ€™s hydration rate (r) from 2.83 x 10-5 to 7.63 x 10-5 mL/s. The hydrogelâ€™s equilibrium weight swelling ratio (qe), protein release and its diffusion coefficient (Dm) were all found to be pH dependent. For example, increasing the phosphate buffer pH from 2.4 to 13 resulted in increased qe from 2 to 16 corresponding to the enlarging of network pore size (Î¾) from 150 to 586 nm.The biomedical potential of the synthesized material based on its accelerative wound healing in rats was observed. Biochemical, histomorphometric and immunohistomorphometric analyses revealed a significant difference in area closure and re-epithelialization on days 7 and 14 in PPH or IntrasiteÂ® gel group compared to gum acacia or PEGMA-alone groups. Furthermore, wounds dressed with PPH or IntrasiteÂ® gel showed evident collagen deposition, enhanced fibrosis and extensively organized angiogenesis on day 14 compared to the negative control group. The findings suggested that topical application of PPH accelerated the ratsâ€™ wound healing process by improving angiogenesis attributed to the increased microvessel density (MVD) and expressions of VEGF-A in tissue samples. Thus, PPH has been demonstrated to be effective in the treatment of cutaneous wounds in rats, and could be a potential novel agent in the management and acceleration of wound healing in humans and animals.
Extraction and Characterization of Chitin and Chitosan from Aspergillus niger, Synthesis of Silver-Chitosan Nanocomposites and Evaluation of their Antimicrobial Potential
Authors: Madhusudhan KN 
Published: August 2018

The fungal species such as Aspergillus niger are the rich source of antimicrobial compounds. The chitin which is the cell wall component are being isolated and used as potential source of antimicrobial along with its derivates. In the present study, the chitin and chitosan were purified from the mycelial mat of the A. niger. By using the chitosan isolated from the fungi, the chitosan-silver nanocomposite was synthesized under lab conditions. The comparative antibacterial potential of chitin, chitosan and nanocomposites were screened against Bacillus subtilis and Bacillus cereus, which are infecting mulberry silkworm (Bombyx mori) and humans respectively. The in vitro inhibition study results revealed that, Nanocomposites showed more inhibition of pathogens in comparison with other treatments along with positive control. Further, the highest antibacterial activity of chitosan-silver nanocomposites may be due to presence of two potential antimicrobial compounds in combination. Based on the results of the present study, chitosan based nanocomposites can be used for the management of the bacterial diseases of silkworm. The nanocomposite derived in the present work can be used for management of medically important pathogens infecting humans.
A comparative Analytical Studies onAcaciapolyacantha gum Samples collected from three different locations in Sudan
Authors: Elhenawy Ahmed , A. Omer Elgaili. , A. Alomari Abdulaziz. , E. Osman Mohammed. 
Published: August 2018

Acacia polyacanthagum(Kakamut gum) samples were collected as natural exudates, from three different locations in SudanÂ named Abugarin, Gargadah and Layoon forest. Physicochemical properties of gum samples were studied (moisture , ash ,nitrogen , protein, specific rotation, intrinsic viscosity , refractive index, equivalent weight, pH , uronic acid and reducing sugars . Results showed significant differences within each location in most parameters studied, refractive index values were found to be constant in all samples (1.3337) . The effect of location on the properties of gum samples was studied and the analysis showed generally insignificant difference (p 0.05) in all properties studied, except moisture and uronic acid showed significant differences. The general characteristics of Acacia gum might be described as a mean value of all properties studied of all gum samples of the three different locations as follows ; 8.3% moisture, 2.9% ash ,0.35%nitrogen, 2.30% protein,-16.8 specific rotation,10.3ml/g intrinsic viscosity, 4.9 pH , 1348.68 equivalent weight,14.5%uronic acid, 1.3337 refractive index and 0.20%reducing sugar . UV absorption spectra of gum samples were determined and the maximum absorption points were found the same ranging between wavelength (208 and 211nm) . The cationic composition of gum samples was also determined and the results showed that sodium(43.075ppm) has the highest value in all samples followed by iron(27.667ppm) ,zinc(14.297ppm),nickel(4.650ppm),copper(4.025),manganese(2.645ppm),calcium(1.284ppm) and trace amount of lead(0.667ppm),potassium(0.344ppm)andmagnesium(0.274ppm). Accordingly, these parameters, therefore, cannot be recommended as qualifying indices.
Children's misconceptions and conceptual change in Physics Education: the concept of light
Authors: Grigorovitch Alyona 
Published: August 2018

The aim of this research project is to investigate the effect of a constructivist Â teaching strategy on 11-12 old children's understanding of light. It explores their understanding of the concept of light as an entity that is transmitted independently if the light source and the final receiver. The study was conducted in three phases: pre-test, teaching intervention, and post-test. The sample consisted of 120 children who were assigned to two groups. The children in the experimental group participated in activities which adopted a constructivist perspective, while the children in the control group participated in activities with the same objectives, but adopting an classical teaching perspective. A statistically significant difference was found, between the pre-test and the post-test, providing evidence for the effect of the constructivist strategy on facilitating children in create model for the concept of light.
SCREENING OF SOME MEDICINAL PLANT EXTRACTS AGAINST Thielaviopsis spp-A DISEASE CAUSING PLANT FUNGUS
Authors: Rajan DR. Jenifer Solomon 
Published: August 2018

Medicinal plants have been used in the prevention, treatment and cure disorders and diseases in ancient times and they provide abundant resources of antimicrobial compounds, has been used for centuries to inhibit the microbial growth. Diseases of medicinal plants were caused by the pathogenic microbes: especially fungi are common throughout the world. Microbial diseases of plants cause malfunctions such as disturb normal functions by degrading enzymes, toxin and growth substances, reduce the yield, survival capacity and resulting to death. In recent times, the medicinal plants were affected by exogenous, endogenous and microbial infections. The medicinal plant plant Argemone mexicana L. (Mexican poppy) is an annual exotic weed flora used for medicine in several countries throut the world and chosen for this study.This plant was frequently affected by many fungal pathogens. The present investigation focuses the isolation of frequent disease causing fungal pathogen(ThielaviopsisÂ spp)from infected leaves and their control measures by using herbal extracts(Acalypha indica ,Catharanthus roseusÂ Â and Murraya koenigii Â ) via. Invitro approach.
RESPONSE OF YIELD AND ITS QUALITY OF SWEET PEPPER PLANT TO CERTAIN BIO-STIMULANTS UNDER TWO TYPES OF SALINITY
Authors: A.A Arafa , M.A Khafagy , A.M Abo-El Kheer , El-Banna M.F 
Published: August 2018

Number of fruits and its fresh weight per plant were significantly increased under low salinity level of NaCl+CaCl2 in the early 4th picking and total yield. While, plants grown under NaCl salinity did not produced fruits in the early 4th pickings. However, increasing salinity levels caused a significant decrease in this respect. In addition, pre-soaking seeds in selected chemicals used at both levels caused a significant increase in sweet pepper fruits number and its fresh weight per plant (early 4th pickings and total yield) under non-saline conditions. Moreover, salicylic acid at 75 mg/L or ascorbic acid at 50 mg/L was more effective in this respect under non-saline or salinity conditions.
Ascorbic acid as well as total carbohydrates concentrations in sweet pepper fruits were increased significantly with increasing salinity levels. In addition, ascorbic acid and total carbohydrates concentrations were greatly increased in plants growing under NaCl followed by NaCl+CaCl2 and CaCl2 as compared to control. On the other hand, pre-soaking seeds in SA, AsA, toco and yeast extract at both levels increased ascorbic acid and total carbohydrates concentrations under non-saline or salinity conditions.Â Furthermore, AsA at both levels or SA at 75 mg/L was more effective in this respect.
Total soluble solids (TSS %) were significantly increased under low salinity level (2000 mg/L), thereafer decreased with increasing salinity level to 4000 mg/L. In addition, the great reduction in TSS % occurred under NaCl at high salinity levels as compared with the other salinity type. Moreover, pre-soaking seeds in chemicals used at both levels increased TSS % under non-saline or saline conditions. Furthermore, SA at 75 mg/L or AsA at 50 mg/L was more effective in this respect.
RESPONSE OF CERTAIN PHYTOCHEMICAL CONSTITUENTS IN SWEET PEPPER LEAVES TO SOME BIO-STIMULANTS UNDER TWO TYPES OF SALINITY
Authors: A.A Arafa , M.A Khafagy , A.M Abo-El Kheer , El-Banna M.F 
Published: August 2018

High salinity level of NaCl and CaCl2 (4000 mg/L) markedly increased whereas the low levels and its combination decreased electrolyte leakage percentage (E.L. %) in sweet pepper leaves as compared to the unstressed plants. In addition, application of all selected bio-regulators used at both applied levels, in most cases, alleviated the harmful effect of salinity on E.L. % especially ascorbic acid at 50 mg/L.
Relative water content (RWC) and photosynthetic pigments were significantly increased thereafter decreased as salinity levels increased. In addition, CaCl2 at low level caused a great increase in RWC % followed by NaCl+CaCl2 and NaCl (2000 mg/L). While, pre-soaking seeds in both levels of the applied bio-stimulants caused a significant increase in RWC % and the photosynthetic pigments concentrations . In addition, AsA at 50 mg/L or SA at 75 mg/L was more effective in this respect as compared to the untreated plants.
Ascorbic acid, proline, total phenols as well as total soluble carbohydrates concentrations in sweet pepper shoot were increased with increasing salinity levels from 2000 to 4000 mg/L of all salinity types. In addition, NaCl led to a great increase followed by NaCl+CaCl2 and CaCl2 as compared to the unstressed plants. In addition, pre-soaking seeds in SA, AsA, tocopherol and yeast extract at both levels increased ascorbic acid, proline, total phenols as well as total soluble carbohydrates concentrations under saline conditions. Moreover, AsA at 50 mg/L or SA at 75 mg/L was more effective in this respect as compared with the other treatments
Kinetics of Electron Exchange between Bromopyrogallol Red and Periodate Ion in Aqueous Acidic Medium
Authors: Myek Bako , Idris S. O , Adeyemo G.B , Iyun J. F 
Published: August 2018

The kinetics of the electron transfer reaction between Bromopyrogallol (BPR2-) and periodate ion in aqueous acidic solution has been studied in the acid range 0.1 — 10-4 â‰¤ [H+] â‰¤ 2 — 10-4 mol dm-3, ionic strength 0.01 â‰¤ [I] â‰¤ 0.18 mol dm-3 (NaCl) and T = 29 ± 1.ºC. The reaction shows a first order dependence on reductant and half order on oxidant concentration respectively. It displayed an inverse dependence on the acid concentration of the reaction medium. The reaction conforms to the rate law:
-d[BPR2-]/dt = k2[BPR2-][IO4-]1/2[H+]-1/2
The stoichiometry of the reaction is 1:1. Added anions had no effect on the rate of the reaction. Spectroscopic investigation indicates that an intermediate complex is probably formed in the course of this reaction. The reaction is believed to proceed via the innersphere mechanistic pathway.
A study on Volumetric and Viscometric Properties for the Mixtures of DMF and Other Alcohols from Temperatures ranges 303.15K to 323.15K
Authors: Khan Md. Zaved Hossain , Uddin Md Helal , Islam Md Shahrul , Khan G.M Arifuzzaman , Muhit Md. Abdullah Al , Ferdaus Jannatul , Momin Md. Abdul , Rahman SK Shahinur 
Published: August 2018

Densities, p, and viscosities, ?, of four binary systems: N,N-dimethyl formamide (DMF) + 1-propanol, DMF + 2-propanol, DMF + butanol and DMF + 1-pentanol have been measured at five different temperatures ranging form 303.15K to 323.15K at an interval of 5K. Excess molar volumes were found to be negative over the entire concentration ranges. The negative excess molar volume decreases with the increase of chain length of alcohol, the magnitude follows the order DMF+ 1-propanol >, + 2-propanol >, +butanol >, + 1-pentanol. The change of viscosity has been found to be sensitive to the chain length of alcohols. Excess viscosities and Grunberg- Nissan interaction parameters have been found to be positive for DMF + 1-propanol and DMF + 2-propanol systems, and negative for other two systems DMF + butanol and DMF + 1-pentanol. The thermodynamic energy of activation; such as, positive ?G#E each also compatible with the observation.
EFFECT OF VAM FUNGI ON PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY IN SOLANUM SURATTENSE L.
Authors: Pria S , Shanmugapriya O.N. 
Published: August 2018

In this study six fungal species were isolated from soil samples. Among the six species Glomus fasiculatum and Gigaspora marigarita noted in dominant level. So both are used as bioinoculant and were mass multiplied in onion plant by pot culture. After pot culture, biochemical and antibacterial activity were analysed from the treated plant Solanum surattense. In the phytochemical analysis falvonoids, tannins , terpenoids, cardiac glycosides and saponins were noted. Antibacterial activity of ethanolic extract of Solanum surattenseÂ against clinical isolates were analyzed and show the more activity noted in K.pneumoniae when compared with Bacillus subtilis, Streptococcus pyogens, E.coli respectively.
SENSITIVITY PATTERNS OF SOME FLOWERING PLANTS AGAINST SALMONELLA TYPHI AND PSEUDOMONAS AERUGINOSA
Authors: Rajan DR. Jenifer Solomon 
Published: August 2018

The medicinal plants have been collected form wild resources and only some species used in larger quantities are cultivated systematically. Many medicinal plants, which were ignored in the past years, but the number of plants still formed in the wild is progressively declining the medicinal value with this situation to acquiring from the wild sources in more important recent trends bening. Reported in cresingly the antimicrobial properties of medicinal plants from different parts of the world .On this basis, the present investigation was focused on the screening of antimicrobial properties ofAsystasia indica Asystasia gangetica Thunbergia alata on selected Pathogenic bacteriaThe main objectives of this work use as follow To Screen the antimicrobial properties of these plants on selected micro organisms.To determine the effectiveness of inhibition on microbes by comparing its activity with known antibiotics.