bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimated potassium intake and the progression of chronic kidney disease
Authors: Suenaga Tatsuya , Tanaka Shigeru , Kitamura Hiromasa , Tsuruya Kazuhiko , Nakano Toshiaki , Kitazono Takanari 
Published: November 2024

ABSTRACT

Background
Lower potassium intake is associated with a higher risk of chronic kidney disease (CKD) in the general population. However, there are no stated recommendations on potassium intake in the CKD population owing to limited evidence of benefits from potassium intake and concerns about the risk of hyperkalaemia. This study aimed to investigate the relationship between potassium intake and CKD progression.

Methods
A total of 4314 patients aged 18 years or older in Japan were prospectively followed for 5 years using data from the Fukuoka Kidney Disease Registry study. The patients were divided into quartiles according to estimated potassium intake levels assessed by the Tanaka formula from spot urine samples. The primary outcome was CKD progression, which was defined as a composite of a 1.5-fold increase in creatinine concentrations from baseline and development of end-stage kidney disease. We evaluated the relationship between estimated potassium intake and CKD progression using Cox proportional hazards models.

Results
A total of 1490 patients showed CKD progression during the follow-up with an incidence rate of 90.1/1000 person-years. Patients in the lowest estimated potassium intake quartile had higher hazard ratios for CKD progression than those in the highest quartiles in the multivariable-adjusted Cox models [hazard ratio (95% confidence interval) 1.24 (1.03–1.48)]. Similarly, each 1-standard deviation decrease in estimated potassium intake as a continuous variable was associated with a higher risk of CKD progression [hazard ratio (95% confidence interval) 1.10 (1.03–1.19)].

Conclusions
Lower estimated potassium intake is associated with CKD progression in patients with CKD. Therefore, we recommend adequate potassium intake, taking care not to cause serious adverse events.
Association Between Monounsaturated Fatty Acid Intake and Risk of Total Stroke and Its Subtypes: A Systematic Review and Dose–Response Meta-analysis of Prospective Cohort Studies
Authors: Mehrabani Amirmasoud , Jalalzadeh Moharam , Jannati Nooshin , Lotfi Keyhan , Arzhang Pishva , Azadbakht Leila 
Published: November 2024

Abstract

Context
There are contradictory results regarding the relationship between dietary intakes of monounsaturated fatty acids (MUFAs) and risk of stroke.

Objective
The purpose of this study was to review the existing body of research on the relationship between MUFA consumption and stroke risk through a systematic review and meta-analysis.

Data Source
PubMed, Scopus, and ISI Web of Science were all systematically searched up to January 2023.

Data Extraction
A total of 11 prospective cohort studies that investigated MUFA consumption in relation to stroke risk were included.

Data Analysis
Relative risk (RR) and 95% CIs for the highest vs lowest category of dietary MUFA intake were combined using a random-effects model. Linear and nonlinear dose–response relations were assessed through 1-stage weighted mixed-effects meta-analysis. These studies included 475 207 participants and 8438 cases. In highest vs lowest consumption analysis, MUFA intake was inversely associated with total stroke (RR: 0.80; 95% CI: 0.67, 0.96; I2 =65.2%, Q-test P = .001; n = 11) and hemorrhagic stroke (RR: 0.80; 95% CI: 0.68, 0.95; I2 = 0.0%, Q-test P = .77; n = 5) risk. Conversely, this association was not significant for ischemic stroke (RR: 0.86; 95% CI: 0.69, 1.07; I2 =62.3%, Q-test P = .01; n = 8). An inverse association was observed between each 10-g/d MUFA intake and hemorrhagic stroke (RR: 0.79; 95% CI: 0.65, 0.96) but not total and ischemic stroke. We did not find any evidence for a nonlinear association between MUFA intake and total stroke and its subtypes.

Conclusion
This meta-analysis shows that higher MUFA intake could lower the risk of total and hemorrhagic, but not ischemic, stroke. This could be important for public health.

Systematic Review Registration
PROSPERO registration no. CRD42023458555.
Levofloxacin Prophylaxis in Pediatric and Young Adult Allogeneic Hematopoietic Stem Cell Transplantation Recipients Does not Prevent Infective Complications and Infections-related Deaths
Authors: Leardini Davide , Gambuti Giacomo , Muratore Edoardo , Baccelli Francesco , Gottardi Francesca , Venturelli Francesco , Belotti Tamara , Prete Arcangelo , Fabbrini Marco , Brigidi Patrizia , Turroni Silvia , Masetti Riccardo 
Published: November 2024

Abstract

Background
The prophylactic use of quinolones in the setting of allogeneic hematopoietic stem cell transplantation (allo-HCT) is controversial and solid evidence is missing, particularly in children.

Methods
In this single-center retrospective study, we compared outcomes in patients receiving (n = 74) or not receiving (n = 70) levofloxacin (LVX) prophylaxis, assessing overall survival, event-free survival, acute graft-versus-host disease (aGvHD) and bloodstream infection incidence, and infection-related mortality. Gut microbiota composition was analyzed in a subgroup using 16S rRNA sequencing of stool samples collected pre-HCT and at engraftment.

Results
We analyzed 144 allo-HCT in 143 patients performed for any indication. No differences were found in the 2 groups regarding main HCT outcomes, namely, cumulative incidence of aGvHD (37.9% vs 43.5%; P = .733), grade III-IV aGvHD (12.2% vs 8.7%; P = .469), gut aGVHD (12.2% vs 17.5%; P = .451), bloodstream infections (25.6% vs 34.1%; P = .236) and death from bacterial infection (9.5% vs 4.3%; P = 0.179). In patients experiencing bacterial infections, those receiving prophylaxis showed higher incidence of quinolone-resistant strains (P = .001). On a subgroup of 50 patients, we analyzed the gut microbiota composition, showing a lower abundance of Blautia (P = .015), Enterococcus (P = .011), and Actinomyces (P = .07) at neutrophil engraftment in patients receiving LVX prophylaxis.

Conclusions
LVX prophylaxis in the setting of allo-HCT does not prevent infective complications and increases the prevalence of antibiotic-resistant strains.
Triage performance of DNA methylation for women with high-risk human papillomavirus infection
Authors: Kong Linghua , Xiao Xiaoping , Wu Huanwen , You Yan , Jin Xitong , Liou Yuligh , Liu Pei , Lang Jinghe , Li Lei 
Published: November 2024

Abstract

Objective
DNA methylation is a promising biomarker for cervical cancer screening. This study aimed to validate the triage performance of cytological DNA methylation for detecting cervical intraepithelial neoplasia of grade 3 or worse (CIN3+) in women with high-risk human papillomavirus (hrHPV) infection from a large prospective cohort undergoing opportunistic screening in China (METHY3).

Methods
The triage performance for detecting CIN3+ lesions was compared between HPV16/18 genotyping, a liquid-based cytology (LBC) test, and the PAX1 and JAM3 methylation (PAX1m/JAM3m) test according to cervical pathologic outcomes. Among the 4394 women infected with hrHPV, 1105 had definitive cervical histological findings that were analyzed.

Results
For detecting CIN3+, the specificity of HPV16/18(+), the LBC result of ≥atypical squamous cells of undetermined significance (ASCUS), and PAX1m/JAM3m(+) was 66.4%, 23.9%, and 89.6%, respectively, with odds ratios of 4.24 (95% confidence interval [CI], 2.85-6.40), 4.44 (2.27-10.1), and 18.5 (12.1-28.7) (P &lt; .001), respectively. PAX1m/JAM3m(+) had the highest area under the receiver operating characteristic curve (0.790, 95% CI, 0.747-0.832) in the whole cohort and in women of various ages. PAX1m/JAM3m (+) was detected in all patients with cancer (n = 28). Compared with HPV16/18 genotyping and the LBC test, PAX1m/JAM3m testing reduced referrals to colposcopy by 20.64 percentage points and 61.18 percentage points, respectively.

Conclusions
PAX1 m /JAM3 m testing is highly specific for detecting CIN3+. As a triage biomarker, it is superior to HPV 16/18 genotyping and LBC testing for women with hrHPV infection.
Amide conjugates of the jasmonate precursor cis-(+)-12-oxo-phytodienoic acid regulate its homeostasis during plant stress responses
Authors: Široká Jitka , Ament Anita , Mik Václav , Pospíšil Tomáš , Kralová Michaela , Zhang Chao , Pernisová Markéta , Karady Michal , Nožková Vladimira , Nishizato Yuho , Kaji Takuya , Saito Rina , Htitich Mohamed , Floková Kristýna , Wasternack Claus , Strnad Miroslav , Ueda Minoru , Novák Ondřej , Brunoni Federica 
Published: November 2024

Abstract
Jasmonates are a family of oxylipin phytohormones regulating plant development and growth and mediating “defense versus growth” responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently of CORONATIVE INSENSITIVE 1-mediated JA signaling in several stress-induced and developmental processes. However, its perception and metabolism are only partially understood. An isoleucine analog of the biologically active JA-Ile, OPDA-Ile, was detected years ago in wounded leaves of flowering plants, opening up the possibility that conjugation of cis-OPDA to amino acids might be a relevant mechanism for cis-OPDA regulation. Here, we extended the analysis of amino acid conjugates of cis-OPDA and identified naturally occurring OPDA-Val, OPDA-Phe, OPDA-Ala, OPDA-Glu, and OPDA-Asp accumulating in response to biotic and abiotic stress in Arabidopsis (Arabidopsis thaliana). The OPDA amino acid conjugates displayed cis-OPDA-related plant responses in a JA-Ile-dependent manner. We also showed that the synthesis and hydrolysis of cis-OPDA amino acid conjugates are mediated by members of the amidosynthetase GRETCHEN HAGEN 3 and the amidohydrolase INDOLE-3-ACETYL-LEUCINE RESISTANT 1/ILR1-like families. Thus, OPDA amino acid conjugates function in the catabolism or temporary storage of cis-OPDA in stress responses instead of acting as chemical signals per se.
Co-exposure impact of nickel oxide nanomaterials and Bacillus subtilis on soybean growth and nitrogen assimilation dynamics
Authors: Khan Kashif , Li Zhen Wei , Khan Rayyan , Ali Shahid , Ahmad Haseeb , Shah Muhammad Ali , Zhou Xun Bo 
Published: November 2024

Abstract
Nickel oxide nanoparticles (NiO-NPs) pose potential threats to agricultural production. Bacillus subtilis has emerged as a stress-mitigating microbe that alleviates the phytotoxicity caused by NiO-NPs. However, the mechanisms underlying its effectiveness, particularly in root-nodule symbiosis and biological N2-fixation (BNF), remain unclear. Here, we tested the combined exposure of NiO-NPs (50 mg kg−1) and B. subtilis on soybean (Glycine max L.) growth and BNF. Combined exposure increased root length, shoot length, root biomass, and shoot biomass by 19% to 26%, while Ni (200 mg kg−1) reduced them by 38% to 53% compared to the control. NiO-NPs at 100 and 200 mg kg−1 significantly (P &lt; 0.05) reduced nodule formation by 16% and 58% and Nitrogen assimilation enzyme activities levels (urease, nitrate reductase, glutamine synthetase, and glutamate synthetase) by 13% to 57%. However, co-exposure with B. subtilis improved nodule formation by 22% to 44%. Co-exposure of NiO-NPs (200 mg kg−1) with B. subtilis increased peroxidase, catalase, and glutathione peroxidase activity levels by 20%, 16%, and 14% while reducing malondialdehyde (14%) and hydrogen peroxide (12%) levels compared to NiO-NPs alone. Additionally, co-exposure of NiO-NPs (100 and 200 mg kg−1) with B. subtilis enhanced the relative abundance of Stenotrophomonas, Gemmatimonas, and B. subtilis, is associated with N2-cycling and N2-fixation potential. This study confirms that B. subtilis effectively mitigates NiO-NP toxicity in soybean, offering a sustainable method to enhance BNF and crop growth and contribute to addressing global food insecurity.
Do Financial Concerns Make Workers Less Productive?
Authors: Kaur Supreet , Mullainathan Sendhil , Oh Suanna , Schilbach Frank 
Published: November 2024

Abstract
Workers who are worried about their personal finances may find it hard to focus at work. If so, reducing financial concerns could increase productivity. We test this hypothesis in a sample of low-income Indian piece-rate manufacturing workers. We stagger when wages are paid out: some workers are paid earlier and receive a cash infusion while others remain liquidity constrained. The cash infusion leads workers to reduce their financial concerns by immediately paying off debts and buying household essentials. Subsequently, they become more productive at work: their output increases by 7% (0.11 std. dev.), and they make fewer costly, unintentional mistakes. Workers with more cash on hand thus not only work faster but also more attentively, suggesting improved cognition. These effects are concentrated among more financially constrained workers. We argue that mechanisms such as gift exchange or nutrition cannot account for our results. Instead, our findings suggest that financial strain, at least partly through psychological channels, has the potential to reduce earnings exactly when money is most needed.
A Scoping Review of Multimodal, Dyadic Early Relational Health Interventions in NICUs in the United States
Authors: Darilek Umber , Graw Jasmine , Sisk Laura , Crawford Allison D. , Lopez Emme , Howe Rebecca , McGlothen-Bell Kelly 
Published: November 2024

Background:
Early relational health (ERH) interventions can buffer toxic stress and improve the developmental trajectories of neonatal intensive care unit (NICU) infants.

Purpose:
The purpose was to examine the current state of the science related to multimodal, dyadic ERH interventions implemented in the NICU setting in the United States and identify gaps in the current literature.

Data Sources:
PubMed, Scopus, Cumulative Index to Nursing and Allied Health Literature (CINAHL), and PsycInfo were searched in November 2022 and November 2023 for original studies and conference proceedings from 1970 to present in the English language. Gray literature searches were performed in February 2023 and December 2023.

Study Selection:
English language, original research, with a focus on multimodal, dyadic ERH interventions that took place primarily in a NICU in the United States were included. Outcome measures could be related to implementation, relational health, or physical and/or mental health outcomes of parent and/or infant. Eighteen of 2021 reviewed articles met the inclusion criteria.

Data Extraction:
Data were extracted for author, year, intervention, purpose, methods, sample, paternal inclusion, dyadic components, non-dyadic components, and major outcomes/results and distilled for study characteristics, multimodal, dyadic intervention characteristics, and outcome measures.

Results:
Several multimodal dyadic interventions exist to aid ERH in the NICU, providing evidence of improved outcomes for infants and families. More research is required using higher sample sizes and replication studies.

Implications for Practice and Research:
ERH interventions show promise in improving neurodevelopmental, behavioral, and maternal mental health outcomes and should be considered for implementation into NICU services.
Giving a voice to patient and caregiver perspectives on equity in transplantation
Authors: Reed Rhiannon D. , Locke Jayme E. 
Published: November 2024

Purpose of review
While prior reviews have assessed barriers to transplantation experienced by patients and their families, they have not summarized how these stakeholders feel about whether and how those barriers impact equity in transplantation. We seek to comprehensively present the literature of patients and family perspectives on disparities in transplant access and potential solutions.

Recent findings
Some patients and their families report experiences of discrimination, while others from traditionally marginalized groups feel the system is equitable and do not perceive any different treatment as a result of their race/ethnicity, sex, age, religion, or geography. This summary suggests that not all of the disparities observed in transplant access are attributable to systemic or unconscious bias and may be partially alleviated through enhanced education and communication.

Summary
Lack of understanding and transparency lead to mistrust of organ transplantation, further exacerbating existing disparities. Patient and family engagement is paramount for ensuring the trust of the public who are the source of organs and those who directly benefit from this field. Partnerships between patient advocacy groups, professional societies, and policymakers have the potential to increase transparency while ensuring that the patient voice is represented in any system-level change and assessment.
Flowers that cool themselves: thermal ecology of summer-blooming thistles in hot Mediterranean environments
Authors: Herrera Carlos M. 
Published: November 2024

AbstractFlower exposure to high temperature reduces the production, viability and performance of pollen, ovules and seeds, which in turn reduces individual fecundity and risks the survival of populations. Autonomous flower cooling could help to alleviate exposure of pollen and ovules to harmful temperatures, yet investigations on the thermal ecology of flowers in hot environments are still needed to evaluate the frequency, magnitude and ecological significance of floral thermoregulatory cooling. This paper reports a study on the thermal ecology of flower heads (=capitula) of 15 species of summer-blooming Asteraceae, tribe Cardueae, from hot-dry habitats in the southern Iberian Peninsula. Temperature inside (Tin) and outside (Tout) capitula were assessed under natural field conditions by two complementary sampling and measurement procedures, which provided information on the relationships between the two temperatures at the levels of individual capitula (“continuous recording”) and local plant populations (“instantaneous measurements”). Baselines for theTin–Toutrelationship in absence of physiological activity were obtained by exposing dehydrated capitula to variable ambient temperatures. To assess whether the co-flowering capitula of summer-blooming Asteraceae collectively defined a distinct thermal layer, the vertical distribution of capitula relative to the ground was quantified. Bees visiting capitula were watched and the temperature of the air beside the visited capitulum was measured. Capitula of all species were exposed to high ambient temperatures during long periods, yet their interior was cooler than the air for most of the time, with temperature differentials (ΔT=Tin-Tout) quite often approaching, and sometimes exceeding −10°C. The relationship betweenTinandToutwas best described by a composite of steep and shallow linear relationships separated by a breakpoint (Ψ, interspecific range=25°C–35°C). Capitula were weakly or not thermoregulated forTout<Ψ, but switched to intense, thermoregulated cooling whenTout>Ψ. Narrow vertical distribution of capitula above ground and similar cooling responses by all species produced a refrigerated floral layer where most bees foraged atTout>Ψ and presumably visited cooled capitula. Thermoregulatory cooling of capitula can benefit plant reproduction by reducing pollen and ovule exposure to high temperature, but also the populations of insect mutualists and antagonists through habitat improvement via a refrigerated flower layer.OPEN RESEARCH STATEMENTData, metadata and R script will be made available at figshare.
Identification and genomic characterization of a novel bisegmented coronavirus in the lesser panda
Authors: Chen Xiaoyuan , Guo Hua , Zhou Niu , Shi Zhengli , Cui Xinyuan , Dong Tianyi , Qin Mengjie , Liang Xianghui , Shan Fen , Wang Hai , Zuo Qian , Peng Shiming , You Wenjie , Irwin David M. , Ding Chan , Zeng Leiping , Chen Wu , Shen Yongyi 
Published: November 2024

AbstractCoronaviruses (CoVs) are enveloped positive-sense single-stranded RNA viruses and are renowned for their capacity to infect a diverse range of animals, including humans. In this study, we report the identification and characterization of a novel bisegmented coronavirus, designated LpCoV, from a dead lesser panda, which exhibited severe clinical manifestations and lesions in multiple organs. The LpCoV represents the first documented coronavirus with a unique bisegmented genome while maintaining the typical coronavirus morphology. One genomic segment encodes the ORF1ab polyprotein, while the other segment encodes the spike and nucleocapsid proteins, notably lacking the envelope (E) and membrane (M) genes. The pairwise patristic distances of the five concatenated domains in the replicase region (3CLpro, NiRAN, RdRP, ZBD, and HEL1) between LpCoV and α-δ coronaviruses range from 1.19 to 1.70. Based on the classification criteria established by the International Committee on Taxonomy of Viruses (ICTV), LpCoV is proposed to constitute a new genus within the Coronaviridae family. An epidemiological investigation identified five LpCoV-like viruses in lesser pandas from two different provinces (Sichuan and Jiangsu) indicating the long-term circulation and expansion of bisegmented coronaviruses in wildlife. These findings highlight the imperative for comprehensive viral surveillance in wildlife, which is essential for understanding and mitigating the risk of animal diseases and zoonotic spillover.
Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins
Authors: Xu Da , Besselink Swen , Ramadoss Gokul N. , Dierks Philip H. , Lubin Justin P. , Pattali Rithu K. , Brim Jinna I. , Christenson Anna E. , Colias Peter J. , Ornelas Izaiah J. , Nguyen Carolyn D. , Chasins Sarah E. , Conklin Bruce R. , Nuñez James K. 
Published: November 2024

AbstractProgrammable epigenome editors modify gene expression in mammalian cells by altering the local chromatin environment at target loci without inducing DNA breaks. However, the large size of CRISPR-based epigenome editors poses a challenge to their broad use in biomedical research and as future therapies. Here, we present Robust ENveloped Delivery of Epigenome-editor Ribonucleoproteins (RENDER) for transiently delivering programmable epigenetic repressors (CRISPRi, DNMT3A-3L-dCas9, CRISPRoff) and activator (TET1-dCas9) as ribonucleoprotein complexes into human cells to modulate gene expression. After rational engineering, we show that RENDER induces durable epigenetic silencing of endogenous genes across various human cell types, including primary T cells. Additionally, we apply RENDER to epigenetically repress endogenous genes in human stem cell-derived neurons, including the reduction of the neurodegenerative disease associated V337M-mutated Tau protein. Together, our RENDER platform advances the delivery of CRISPR-based epigenome editors into human cells, broadening the use of epigenome editing in fundamental research and therapeutic applications.