bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRISPR-Cas off-target detection using Oxford Nanopore sequencing - is the mitochondrial genome more vulnerable to off-targets?
Authors: Chakraborty Sandeep 
Published: August 2019

AbstractOxford Nanopore sequencing of DNA molecules is fast gaining popularity for generating longer reads, albeit with higher error rates, in much lesser time, and without the error introduced by PCR-amplification. Recently, CRISPR-Cas9 has been used to enrich genomic regions (nCATS [1]). This was applied on 10 genomic loci (median length=18kb). Here, using the sequencing data (Accid:PRJNA531320), it is shown that the same flow can be used to identify CRISPR-Cas9 off-target edits (OTE). OTEs are an important, but unfortunately underestimated, aspect of CRISPR-Cas gene-editing. An OTE in the mitochondrial genome is shown having 7 mismatches with one of the 10 gRNAs used (GPX1), having as much enrichment as the targeted genomic loci in some samples. Previous study has shown that Cas9 bind to off-targets having as many as 10 mismatches in the PAM-distal region. This OTE has not been reported in the original study (still a pre-print), which states that sequences from parts other than the target locations arise ‘from ligation of nanopore adaptors to random breakage points, with no clear evidence of off-target cleavage by Cas9’ [1], Furthermore, a lot of reads aligning to the mitochondrial genome (sometimes full length) are inverted after the edit. It remains to be seen if these are bona fide translocations after the Cas9 edit, or ONP sequencing artifacts. This also raises the question whether the mitochondrial genome is more prone to off-targets by virtue of being non-nuclear. Another locus in ChrX (13121412) has only 1 mismatch with the second BRAF gRNA (GACCAAGGATTTCGTGGTGA). Although the number of reads for this OTE is less, its very unlikely this is random since it happens 8 out of 11 samples. With the increasing use of (TALEN/ZFN/CRISPR-Cas9) on human subjects, this provides a fast method to quickly query gRNAs for off-targets in cells obtained from the patient, which will have their own unique off-targets due to single nucleotide polymorphism or other variants.
Novel implementation of IoT based non-invasive sensor system for real-time monitoring of intravenous fluid level for assistive e-healthcare
Authors: Ray Partha Pratim , Thapa Nishant , Dash Dinesh , De Debashis 
Published: August 2019

PurposeThe purpose of the study is to design and develop an pervasive and smart Internet of Things (IoT)-based sensor system to monitor he real-time intravenous (IV) fluid bag level.Design/methodology/approachThis paper investigates such issue and performs several experiments to develop a non-invasive, semi-automatic system to monitor IoT-based IV fluid level in real-time.FindingsThe outcome of this study is a prototype hardware that includes an ESP8266 based embedded Web server to disseminate the fluid exhaust status flag to its connected users. Nurses can get the prompt intimation about the status of IV fluid bag whether it is about to get empty.Research limitations/implicationsIoT is the backbone of the proposed system. Multi-master system need to be studied in future.Practical implicationsNon-invasive and real-time IoT-based novel technique is developed with power-efficient and cost-effective pervasive sensors.Social implicationsThis is applicable for pervasive and assistive e-health-care services by care givers and medical professionals.Originality/valueThe deployed system is controlled by ATtiny85 with help of LM35 temperature sensor. The results show a promising future of the proposed development in enhancing IoT-based smart health-care service in the coming days.
A low-PDAP and high-PSNR approximate 4:2 compressor cell in CNFET technology
Authors: Safaei Mehrabani Yavar , Bagherizadeh Mehdi , Shafiabadi Mohammad Hossein , Ghasempour Abolghasem 
Published: August 2019

Purpose
This paper aims to present an inexact 4:2 compressor cell using carbon nanotube filed effect transistors (CNFETs).

Design/methodology/approach
To design this cell, the capacitive threshold logic (CTL) has been used.

Findings
To evaluate the proposed cell, comprehensive simulations are carried out at two levels of the circuit and image processing. At the circuit level, the HSPICE software has been used and the power consumption, delay, and power-delay product are calculated. Also, the power-delaytransistor count product (PDAP) is used to make a compromise between all metrics. On the other hand, the Monte Carlo analysis has been used to scrutinize the robustness of the proposed cell against the variations in the manufacturing process. The results of simulations at this level of abstraction indicate the superiority of the proposed cell to other circuits. At the application level, the MATLAB software is also used to evaluate the peak signal-to-noise ratio (PSNR) figure of merit. At this level, the two primary images are multiplied by a multiplier circuit consisting of 4:2 compressors. The results of this simulation also show the superiority of the proposed cell to others.

Originality/value
This cell significantly reduces the number of transistors and only consists of NOT gates.
The opportunity and challenge of trust and decision-making uncertainty
Authors: Wu Li-Wei , Wang Chung-Yu , Rouyer Ellen 
Published: August 2019

Purpose
Value has been conceptualized as the result of co-creation involving service firms and customers. Currently, however, little is known about why and how customers engage in value co-creation with a service firm. Thus, the purpose of this paper is to explore the role of co-production in value co-creation in the context of banking services from the customers’ viewpoint. The literature has consistently examined the linear effects of trust and decision-making uncertainty on co-production. The study extends this research stream by considering the negative quadratic effects of trust and decision-making uncertainty on co-production. Therefore, this study not only examines the linear and negative quadratic effects of trust and decision-making uncertainty on co-production within a single, simultaneous model but also tests the effect of co-production on value co-creation. Moreover, this study includes and explores the moderating effects of service innovativeness and service effort on co-production in determining value co-creation.

Design/methodology/approach
The hierarchical moderated regression was used to test the hypotheses.

Findings
The findings support the positive linear effects and negative quadratic effects among trust, decision-making uncertainty and co-production. Meanwhile, the results indicate that co-production positively affect value co-creation. Service innovativeness and service effort enhance the effect of co-production on value co-creation.

Originality/value
This study shows the presence of the opportunity of trust and decision-making uncertainty, which confirms the existing literature, and the challenge of trust and decision-making uncertainty, which extends the literature. This study is the first one to shed light on the negative quadratic effects of trust and decision-making uncertainty on co-production. This study also offers insights into value co-creation and thus enhances the current understanding of value phenomena. Academics and practitioners would greatly benefit from a comprehensive understanding of co-production and the associated value co-creation for the parties involved.
Towards a hierarchy of hotel guests’ in-room needs
Authors: Ineson Elizabeth M. , Čomić Djordje , Kalmić Lazar 
Published: August 2019

Purpose
This paper aims to examine, discuss and intertwine theory and practice related to hotel guests’ home and lodging environments to identify their in-room psychological and physical needs.

Design/methodology/approach
The research design is qualitative, with a focus on understanding social phenomena through direct observation, communication and textual analysis; contextual subjective accuracy is stressed over generality. Using interpretative phenomenology, the psychological and sociological aspects of the individual’s journey are deliberated including: travelling through one’s own room; virtual travels from the room via electronic media; the hotel room as a reconstruction of the intimate sphere; the return to, and transformation of, one’s own room; and the accumulation of souvenirs. The tabulated findings are linked to hotel guests’ in-room needs using inductive thematic coding and content analysis.

Findings
The psychological and physical in-room needs of individual hotel guests are identified; safety, security and control emerge as paramount. Practical suggestions to complement, expand and enrich guests’ in-room experiences are offered.

Research limitations/implications
As the focus is limited predominantly to the debate and rationalisation of concepts and the methodology is exploratory as opposed to scientific, the validity of the findings may be questioned; primary data triangulation modifies this criticism. Future researchers are advised to consider the evidence when developing hypotheses to establish a theory of lodging. Research on improving the quality of facilities and services should centre on an analysis of the concept of “being in one’s own room”, followed by an analysis of the transposition to “being in a hotel room” from both physiological and psychological perspectives.

Practical implications
Referring to the developed hierarchy, accommodation/lodging providers are recommended to increase their efforts to exceed the expectations of individual guests. Guest profiling should focus on individual needs and preferences, ideally at the booking stage.

Social implications
Accommodation/lodging providers are encouraged to demonstrate social awareness and empathy through individual social responsibility efforts to enhance the experiences and quality of life of their guests.

Originality/value
The paper makes a valuable contribution by debating and connecting philosophical and psychological literature to the practical needs of hotel guests. It recognises and concludes that, as they are merely temporary residents, hotel guests’ experiences embrace a series of journeys through different, individual interiors. Based on this premise, an innovative hierarchy of hotel guests’ in-room needs is developed.
Leadership curricula in UAE business and education management programmes
Authors: ElKaleh Eman 
Published: August 2019

Purpose
The purpose of this paper is to investigate leadership curricula in UAE business and education management programmes and examine the extent to which they are derived from and linked to students’ cultural and Islamic values using Habermas’ critical theory.

Design/methodology/approach
The study employs a mixed methods approach that takes classical pragmatism as its philosophical foundation and critical theory as a theoretical lens. Data are collected in four sequential phases using critical discourse analysis of course materials, class observations, student survey and faculty interviews. Results are integrated at the interpretative level and abductive reasoning is used as the logic of justification.

Findings
Results show that despite the increasing efforts to incorporate cultural and Islamic values into the curriculum, it is still mainly dominated by Western theories and models of leadership, especially in the leadership courses offered by business schools, mainly because of accreditation requirements and the lack of English resources and theories on UAE and Islamic models of leadership.

Research limitations/implications
The study is limited to leadership curricula in the UAE. Researchers may extend and broaden the scope of the study by investigating leadership curricula in the Gulf and/or the Middle East. Future studies may also look at other theoretical frameworks recommended by other management scholars such as Mezirow’s transformational learning and the socio-constructivist approach (Hotho and Dowling, 2010). This study aims to open an ongoing debate and further investigation on the topic.

Practical implications
The results of the current study may inspire faculty members and programme coordinators to develop critical and culturally relevant curricula that are informed by Habermas’ critical theory and best teaching practices.

Originality/value
The study adds to the current knowledge base through its research design and approach that address an under-investigated topic. None of the current studies empirically investigated leadership curricula in the UAE. The theoretical framework and research findings can be used to develop culturally relevant and value-oriented leadership curricula that reflect indigenous and Western perspectives of leadership.
Calibration of a six-axis parallel manipulator based on BP neural network
Authors: Zhang Dianjin , Zhang Guangyu , Li Longqiu 
Published: August 2019

Purpose
This paper aims to provide a simple and flexible calibration method of parallel manipulators for improving the position accuracy only using partial pose information.

Design/methodology/approach
The overall idea of this method is to use BP neural network to fit the relationship between calibration parameters and measurement parameters and then adjust calibration parameters according to measurements.

Findings
The calibration method significantly improves the position accuracy of the six-axis parallel manipulator. Simulation shows that the accuracy can be improved by increasing the number of positions consisted of samples to train BP neural network, and when the position number is increased, the descent velocity of fitting error is decreased.

Originality/value
The method is general for various parallel mechanisms and simple for measurement process. It can be applied to the calibration of various mechanisms without analyzing the mathematical relationship between measurements and calibration parameters. The measurement parameters can be flexibly selected to simplify measurement process, which saves calibration cost and time.
Diversity and inclusion: corporate communications advances the practice
Authors: Pellecchia Ray 
Published: August 2019

Purpose
The purpose of this paper is to make the case that corporate communications (CorpComms) can and should play an important role in diversity and inclusion (D&I). The paper identifies a number of benefits from CorpComms becoming “invested” in D&I and several ways to help communicators advance from just “reporting” on diversity to becoming an engaged part of the effort.

Design/methodology/approach
This study offers perspectives and recommendations based on the author’s three decades in CorpComms, two decades of which have also included serving as a member on corporate diversity councils.

Findings
D&I is often viewed as human resources’ (HR’s) “job” rather than an integral part of everyone’s own work. Such a view risks limiting the success of D&I programs and that of departments and organizations. Nowhere is this more apparent than with CorpComms, a function that involves new and different ideas, active listening and articulating the authentic story of an organization – all activities that align with D&I. Becoming a committed part of the diversity effort can offer communicators new perspectives, fuller context, deeper understanding and the opportunity to give voice to people and ideas that otherwise might not be heard.

Originality/value
For a group of professionals who are animated by the meaningful connection among people, it is difficult to conceive of more affirming and essential work.
Teacher collaboration, differing expectations, and emotions in school improvement
Authors: Weddle Hayley , Lockton Marie , Datnow Amanda 
Published: August 2019

PurposeWhile the benefits of teacher collaboration are well documented, less is known about how emotions intersect with teachers’ collective work. Educational change is an emotional process, as reform efforts often involve shifts in teachers’ daily routines and professional identities. To better understand these complexities, the purpose of this paper is to investigate the emotional dimensions of teachers’ collaborative efforts to improve instruction.Design/methodology/approachDrawing on qualitative data, this longitudinal case study of one teacher team explores how teacher collaboration for instructional improvement intersects with emotional geographies. Data analyzed include three years of meeting observations and annual interviews with teachers and school leaders.FindingsAn analysis of data reveals how emotions both shaped and were shaped by teachers’ collaboration experiences. Varying beliefs about practice, expectations about collective work and identity (in this case, gender) impacted collaboration and subsequently opportunities for instructional improvement.Practical implicationsThis study demonstrates how attending to the emotional aspects of teacher collaboration could serve as an effective strategy for bolstering capacity-building efforts. Findings highlight the interplay between emotional geographies, suggesting that common ground across one geography could potentially be built upon to close gaps across others.Originality/valueThis study provides a unique longitudinal exploration of the emotional dimensions of teachers’ collective work. The study also contributes to new knowledge about the ways in which teachers’ emotions and collaborative experiences intersect, including the interplay between emotional geographies.
Are smart beta funds really smart? Evidence from rational and quasi-rational investor sentiment data
Authors: Verma Rahul , Soydemir Gökçe , Huang Tzu-Man 
Published: August 2019

Purpose
The purpose of this paper is to examine the relative effects of rational and quasi-rational sentiments of individual and institutional investors on a set of smart beta fund returns. The magnitudes of the impacts of institutional investor sentiments are greater than those of individual investor sentiments. In addition, both rational and quasi-rational sentiments of individual and institutional investors have significant impacts on smart beta fund returns. The magnitudes of the impacts of quasi-rational sentiments are greater than those of the rational sentiments for both types of investors (quasi-rational sentiments of institutional investors have the maximum impact). These results are consistent with the arguments that professional investors consider the sentiments of individual investors as contrarian leading indicators which are mainly driven by noise while conform the sentiments of institutional investors which are driven by more rational factors. A majority of smart beta funds in the sample outperform the S&P500 returns in the short term but fail to consistently beat the market. The authors find evidence that smart beta funds with consistently high returns are relatively less (more) driven by individual (institutional) investor sentiments. Overall, the authors argue that smart beta funds appear to follow quasi-rational sentiments of both individual and institutional investors that are not rooted in economic fundamentals.

Design/methodology/approach
The results of the impulse functions generated from a multivariate model suggest that the smart beta fund returns are negatively (positively) impacted by individual (institutional) investor sentiments.

Findings
The magnitudes of the impacts of institutional investor sentiments are greater than those of individual investor sentiments. In addition, both rational and quasi-rational sentiments of individual and institutional investors have significant impacts on smart beta fund returns. The magnitudes of the impacts of quasi-rational sentiments are greater than those of the rational sentiments for both types of investors (quasi-rational sentiments of institutional investors have the maximum impact).

Originality/value
These results are consistent with the arguments that professional investors consider the sentiments of individual investors as contrarian leading indicators which are mainly driven by noise while conform the sentiments of institutional investors which are driven by more rational factors. A majority of smart beta funds in the sample outperform the S&P500 returns in the short term but fail to consistently beat the market. The authors find evidence that smart beta funds with consistently high returns are relatively less (more) driven by individual (institutional) investor sentiments. Overall, the authors argue that smart beta funds appear to follow quasi-rational sentiments of both individual and institutional investors that are not rooted in economic fundamentals.
Are national tourism organisations past their sell-by date? A perspective article
Authors: Hay Brian 
Published: August 2019

Purpose The purpose of this paper is to explore the sustainability of National Tourism Organisations (NTOs) in light of the increasing power of cities.

Design/methodology/approach Based on a review of major societal trends and the author's interpretation of such trends, a tourism futurists’ perspective was applied to better understand the implications of such trends.

Findings The future growth of tourism will be constrained by some of the greatest challenges facing society, including unrelenting population growth, ageing demographics, shortages of natural resources, devolution of power and increasing urbanisation. As a result of such trends, this review suggests there will be resurgence in the power of cities, leading to an increase in tourism between not only global cities but also, more importantly, secondary cities.

Research limitations/implications The results are limited by selection of the trends and the author's interpretations of such trends.

Practical implications The review suggests that city-city marketing will be a key driver in future tourism marketing, over country-country marketing.

Originality/value The paper concludes that NTOs are no longer fit for purpose, as power, trust and relevance have shifted in favour of individual city marketing bureaus, over state controlled NTOs.