bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting PFKFB3 alleviates cerebral ischemia-reperfusion injury in mice
Authors: Burmistrova Olga , Olias-Arjona Ana , Lapresa Rebeca , Jimenez-Blasco Daniel , Eremeeva Tatiana , Shishov Dmitry , Romanov Sergei , Zakurdaeva Kristina , Almeida Angeles , Fedichev Peter O. , Bolaños Juan P. 
Published: August 2019

AbstractThe glycolytic rate in neurons is low in order to allow glucose to be metabolized through the pentose-phosphate pathway (PPP), which regenerates NADPH to preserve the glutathione redox status and survival. This is controlled by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3), the pro-glycolytic enzyme that forms fructose-2,6-bisphosphate, a powerful allosteric activator of 6-phosphofructo-1-kinase. In neurons, PFKFB3 protein is physiologically inactive due to its proteasomal degradation. However, upon an excitotoxic stimuli, PFKFB3 becomes stabilized to activate glycolysis, thus hampering PPP mediated protection of redox status leading to neurodegeneration. Here, we show that selective inhibition of PFKFB3 activity by the small molecule AZ67 prevents the NADPH oxidation, redox stress and apoptotic cell death caused by the activation of glycolysis triggered upon excitotoxic and oxygen-glucose deprivation/reoxygenation models in mouse primary neurons. Furthermore, in vivo administration of AZ67 to mice significantly alleviated the motor discoordination and brain infarct injury in the middle carotid artery occlusion ischemia/reperfusion model. These results show that pharmacological inhibition of PFKFB3 is a suitable neuroprotective therapeutic strategy in excitotoxic-related disorders such as stroke.
HER2, chromosome 17 polysomy and DNA ploidy status in breast cancer; a translational study
Authors: Halilovic Altuna , Verweij Dagmar I. , Simons Annet , Stevens-Kroef Marian J. P. L. , Vermeulen Susan , Elsink Janet , Tops Bastiaan B. J. , Otte-Höller Irene , van der Laak Jeroen A. W. M. , van de Water Carlijn , Boelens Oliver B. A. , Schlooz-Vries Margrethe S. , Dijkstra Jeroen R. , Nagtegaal Iris D. , Tol Jolien , van Cleef Patricia H. J. , Span Paul N. , Bult Peter 
Published: August 2019

AbstractBreast cancer treatment depends on human epidermal growth factor receptor-2 (HER2) status, which is often determined using dual probe fluorescence in situ hybridisation (FISH). Hereby, also loss and gain of the centromere of chromosome 17 (CEP17) can be observed (HER2 is located on chromosome 17). CEP17 gain can lead to difficulty in interpretation of HER2 status, since this might represent true polysomy. With this study we investigated whether isolated polysomy is present and how this effects HER2 status in six breast cancer cell lines and 97 breast cancer cases, using HER2 FISH and immunohistochemistry, DNA ploidy assessment and multiplex ligation dependent probe amplification. We observed no isolated polysomy of chromosome 17 in any cell line. However, FISH analysis did show CEP17 gain in five of six cell lines, which reflected gains of the whole chromosome in metaphase spreads and aneuploidy with gain of multiple chromosomes in all these cases. In patients’ samples, gain of CEP17 indeed correlated with aneuploidy of the tumour (91.1%; p < 0.001). Our results indicate that CEP17 gain is not due to isolated polysomy, but rather due to widespread aneuploidy with gain of multiple chromosomes. As aneuploidy is associated with poor clinical outcome, irrespective of tumour grade, this could improve future therapeutic decision making.
Subwavelength acoustic monopole source emission enhancement through dual gratings
Authors: Mei Jun , Wu Ying 
Published: August 2019

AbstractAcoustic source emission rate is generally low at low frequencies. In this work, we propose a simple design of ‘LEGO’-type acoustic metamaterial that can significantly enhance the low frequency emission rate of an acoustic monopole source. Such enhancement is resulted from the coupling between resonances of a cavity and a dual grating comprised of two concentric layers of periodically distributed narrow slits. We develop an effective medium model to characterize the enhancement. Because of its simple structure, the metamaterial is easy to fabricate and thus facilitates the applications in various domains such as oil exploration.
Flexible model of network embedding
Authors: Fernández-Gracia Juan , Onnela Jukka-Pekka 
Published: August 2019

AbstractThere has lately been increased interest in describing complex systems not merely as single networks but rather as collections of networks that are coupled to one another. We introduce an analytically tractable model that enables one to connect two layers in a multilayer network by controlling the locality of coupling. In particular we introduce a tractable model for embedding one network (A) into another (B), focusing on the case where network A has many more nodes than network B. In our model, nodes in network A are assigned, or embedded, to the nodes in network B using an assignment rule where the extent of node localization is controlled by a single parameter. We start by mapping an unassigned “source” node in network A to a randomly chosen “target” node in network B. We then assign the neighbors of the source node to the neighborhood of the target node using a random walk starting at the target node and with a per-step stopping probability q. By varying the parameter q, we are able to produce a range of embeddings from local (q = 1) to global (q → 0). The simplicity of the model allows us to calculate key quantities, making it a useful starting point for more realistic models.
Fine-grained simulations of the microenvironment of vascularized tumours
Authors: Fredrich Thierry , Rieger Heiko , Chignola Roberto , Milotti Edoardo 
Published: August 2019

AbstractOne of many important features of the tumour microenvironment is that it is a place of active Darwinian selection where different tumour clones become adapted to the variety of ecological niches that make up the microenvironment. These evolutionary processes turn the microenvironment into a powerful source of tumour heterogeneity and contribute to the development of drug resistance in cancer. Here, we describe a computational tool to study the ecology of the microenvironment and report results about the ecology of the tumour microenvironment and its evolutionary dynamics.
Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis
Authors: Keshtkar Somayeh , Kaviani Maryam , Jabbarpour Zahra , Geramizadeh Bita , Motevaseli Elahe , Nikeghbalian Saman , Shamsaeefar Alireza , Motazedian Nasrin , Al-Abdullah Ismail H. , Ghahremani Mohammad Hossein , Azarpira Negar 
Published: August 2019

AbstractIslets transplantation, as a treatment of type 1 diabetes, faces challenges, including the loss of islets in the process of isolation and pre-transplantation due to cellular stresses-induced apoptosis. Accordingly, the optimization of culture plays a decisive role in the transplantation success. In this study, we evaluated the effect of nobiletin on the cultured human islets. Isolated human islets were treated by different concentrations of nobiletin and cultured for 24 and 72 hours. Then, the islets viability, apoptosis, insulin and C-peptide secretion, and apoptosis markers were evaluated. Also, the production of reactive oxygen species (ROS), hypoxia inducible factor 1 alpha (HIF-1α), and its target genes in the islets were examined. Our findings showed that the islets were encountered with hypoxia and oxidative stress after isolation and during culture. These insults induced apoptosis and reduced viability during culture period. Moreover, the secretion of insulin and C-peptide decreased. Nobiletin treatments significantly improved the islets survival through reduction of HIF-1α and ROS production and suppression of apoptosis, along with increased islets function. Islet protective effect of nobiletin might be related to its anti-oxidant, anti-apoptotic and insulinotropic properties. Hence, in order to achieve viable and functional islets for clinical transplantation, the application of nobiletin during pre-transplantation period is useful.
Temperature dependence of nanoscale dynamic processes measured in living millipedes by high resolution inelastic and elastic neutron scattering
Authors: Mamontov Eugene , Osti Naresh C. , Tyagi Madhusudan 
Published: August 2019

AbstractWe have used high energy-resolution neutron scattering to probe nanoscale dynamic processes in living millipedes (Narceus americanus). We have measured the temperature dependence of the intensity of scattered neutrons that do not exchange energy with the living samples on the 1.5 ns time scale, thereby excluding the signal from the highly mobile intra- and extra-cellular bulk-like aqueous constituents in the sample. This measured “elastic” scattering intensity exhibits a non-monotonic temperature dependence, with a noticeable systematic decrease detected between 295 and 303 K on warming up from 283 to 310 K. This decrease demonstrates an excellent inverse correlation with the non-monotonic, as a function of temperature, increase in the slow diffusivity previously observed in planarian flatworms and housefly larvae. This correlation suggests the existence of a biological mechanism, possibly common between different classes (Insects and Myriapods) and even phyla (Arthropods and Platyhelminthes), that dampens the slow nanoscopic dynamics in ectothermic organisms in response to the temperature of the environment exceeding the physiologically optimal range.
Change of Deformation Mechanisms Leading to High Strength and Large Ductility in Mg-Zn-Zr-Ca Alloy with Fully Recrystallized Ultrafine Grained Microstructures
Authors: Zheng Ruixiao , Bhattacharjee Tilak , Gao Si , Gong Wu , Shibata Akinobu , Sasaki Taisuke , Hono Kazuhiro , Tsuji Nobuhiro 
Published: August 2019

AbstractRecently, we have found that fully recrystallized ultrafine-grained (UFG) microstructures could be realized in a commercial precipitation-hardened Magnesium (Mg) alloy. The UFG specimens exhibited high strength and large ductility under tensile test, but underlying mechanisms for good mechanical properties remained unclear. In this study, we have carried out systematic observations of deformation microstructures for revealing the influence of grain size on the change of dominant deformation modes. We found that plastic deformation of conventionally coarse-grained specimen was predominated by {0001} <11–20> slip and {10–12} <10–11> twinning, and the quick decrease of work-hardening rate was mainly due to the early saturation of deformation twins. For the UFG specimens, {10–12} <10–11> twinning was dramatically suppressed, while non-basal slip systems containing <c> component of Burgers vector were activated, which contributed significantly to the enhanced work-hardening rate leading to high strength and large ductility. It was clarified by this study that limited ductility of hexagonal Mg alloys could be overcome by activating unusual slip systems (<c + a> dislocations) in fully recrystallized UFG microstructures.
Telescopic synthesis of cellulose nanofibrils with a stable dispersion of Fe(0) nanoparticles for synergistic removal of 5-fluorouracil
Authors: Sajab Mohd Shaiful , Mohan Denesh , Santanaraj Jude , Chia Chin Hua , Kaco Hatika , Harun Shuhaida , Kamarudin Nur Hidayatul Nazirah 
Published: August 2019

AbstractThe recognition of cellulose nanofibrils (CNF) in the past years as a high prospect material has been prominent, but the impractical cellulose extraction method from biomass remained as a technological barrier for industrial practice. In this study, the telescopic approach on the fractionation of lignin and cellulose was performed by organosolv extraction and catalytic oxidation from oil palm empty fruit bunch fibers. The integration of these techniques managed to synthesize CNF in a short time. Aside from the size, the zeta potential of CNF was measured at −41.9 mV, which allow higher stability of the cellulose in water suspension. The stability of CNF facilitated a better dispersion of Fe(0) nanoparticles with the average diameter size of 52.3–73.24 nm through the formulation of CNF/Fe(0). The total uptake capacity of CNF towards 5-fluorouracil was calculated at 0.123 mg/g. While the synergistic reactions of adsorption-oxidation were significantly improved the removal efficacy three to four times greater even at a high concentration of 5-fluorouracil. Alternatively, the sludge generation after the oxidation reaction was completely managed by the encapsulation of Fe(0) nanoparticles in regenerated cellulose.
The in vivo fate of 225Ac daughter nuclides using polymersomes as a model carrier
Authors: Kruijff R. M. de , Raavé R. , Kip A. , Molkenboer-Kuenen J. , Morgenstern A. , Bruchertseifer F. , Heskamp S. , Denkova A. G. 
Published: August 2019

AbstractIncreasing attention is given to personalized tumour therapy, where α-emitters can potentially play an important role. Alpha particles are ideal for localized cell killing because of their high linear energy transfer and short ranges. However, upon the emission of an α particle the daughter nuclide experiences a recoil energy large enough to ensure decoupling from any chemical bond. These ‘free’ daughter nuclides are no longer targeted to the tumour and can accumulate in normal tissue. In this paper, we used polymersomes as model carrier to evaluate the retention of recoiling daughters of225Acin vivo, and assessed their suitability as therapeutic agents. Vesicles containing225Ac were injected intravenously in healthy mice, and intratumourally in tumour-bearing mice, and the relocation of free213Bi was assessed in different organs upon the injection [225Ac]Ac-polymersomes. The therapeutic effect of225Ac-containing vesicles was studied upon intratumoural injection, where treatment groups experienced no tumour-related deaths over a 115 day period. While polymersomes containing225Ac could be suitable agents for long-term irradiation of tumours without causing significant renal toxicity, there is still a significant re-distribution of daughter nuclides throughout the body, signifying the importance of careful evaluation of the effect of daughter nuclides in targeted alpha therapy.
Prevention and Therapy of Preterm Birth. Guideline of the DGGG, OEGGG and SGGG (S2k Level, AWMF Registry Number 015/025, February 2019) – Part 2 with Recommendations on the Tertiary Prevention of Preterm Birth and the Management of Preterm Premature Rupture of Membranes
Authors: Berger Richard , Abele Harald , Bahlmann Franz , Bedei Ivonne , Doubek Klaus , Felderhoff-Müser Ursula , Fluhr Herbert , Garnier Yves , Grylka-Baeschlin Susanne , Helmer Hanns , Herting Egbert , Hoopmann Markus , Hösli Irene , Hoyme Udo , Jendreizeck Alexandra , Krentel Harald , Kuon Ruben , Lütje Wolf , Mader Silke , Maul Holger , Mendling Werner , Mitschdörfer Barbara , Nicin Tatjana , Nothacker Monika , Olbertz Dirk , Rath Werner , Roll Claudia , Schlembach Dietmar , Schleußner Ekkehard , Schütz Florian , Seifert-Klauss Vanadin , Steppat Susanne , Surbek Daniel 
Published: August 2019

Abstract
Aims This is an official guideline of the German Society for Gynecology and Obstetrics (DGGG), the Austrian Society for Gynecology and Obstetrics (ÖGGG) and the Swiss Society for Gynecology and Obstetrics (SGGG). The aim of this guideline is to improve the prediction, prevention and management of preterm birth based on evidence obtained from recently published scientific literature, the experience of the members of the guideline commission and the views of self-help groups.
Methods The members of the participating medical societies and organizations developed Recommendations and Statements based on the international literature. The Recommendations and Statements were adopted following a formal consensus process (structured consensus conference with neutral moderation, voting done in writing using the Delphi method to achieve consensus).
Recommendations Part 2 of this short version of the guideline presents Statements and Recommendations on the tertiary prevention of preterm birth and the management of preterm premature rupture of membranes.
Overcoming the Charged Ion Competition in Triple Quad Chemical Ionization Ion Source for Estimation of Celecoxib in Biological Matrix and its Application to Bioequivalence Study
Authors: Dubey Naveen Kr , Haq Kashif Ul , Fozdar Bharat I. 
Published: August 2019

AbstractThe purpose of the study was to develop and validate a sensitive, selective and reproducible method for the estimation of Celecoxib in human plasma. During the development, ion competition phenomenon observed in electrospray ionization ion source of liquid chromatography mass spectrometer system and in order to overcome it, different approaches applied. The Celecoxib and Celecoxib D7 in plasma were extracted by liquid-liquid extraction and separated in less than 4.0 min on a reverse phase C18 column, using isocratic elution with a binary mobile phase in the ratio of 70:30 v/v (Acetonitrile: 0.1% Ammonia Solution). Mass spectrometer was used as detector to quantitate the analytes in positive ion mode, using Atmospheric Pressure Chemical Ionization mode. Since the method was developed to perform the pharmacokinetic end point study for United States and Europe market, the method was fully validated, complying Food and Drug Administration, European Medicines Agency guideline and recommendations of American Association of Pharmaceutical Scientists white papers.
De Novo Subtelomeric 6p25.3 Deletion with Duplication of 6q23.3-q27: Genotype–Phenotype Correlation
Authors: Atli Emine Ikbal , Gurkan Hakan , Atli Engin , Vatansever Ulfet , Acunas Betul , Mail Cisem 
Published: August 2019

AbstractDuplications of 6q and deletions of 6p have been reported in more than 30 cases of live born infants and given rise to widespread abnormalities recognizable as a specific clinical syndrome. Different phenotypes have been described with variable clinical signs. Most cases involve the coexistence of unbalanced translocations affecting one or the other of the chromosomes. However, duplication of both chromosome 6q and deletion of 6p regions have been reported in only a few cases. Here, we report the first duplication of chromosome band 6q23.3–q27 with deletion of 6p25.3. This is the first case in the literature involving changes to these specific chromosomal regions; a medium size duplication of the distal long arm and smaller deletion of the terminal short arm of chromosome 6. In the literature, there are no other cases where these two specific chromosomal aberrations are observed together. Conventional chromosome analysis was performed to investigate the patient. Chromosome structure was identified using fluorescence in situ hybridization for subtelomeric regions of chromosome 6 and array comparative genomic hybridization analysis (array-CGH).
PHACE Syndrome: A Rare Case
Authors: Taslicay Ceylan Altintas , Dervisoglu Elmire , Ciftci Ercument , Corapcioglu Funda , Anik Yonca 
Published: August 2019

AbstractPHACE syndrome (OMIM 606519) is a rare neurocutaneous vascular disorder, characterized by posterior fossa malformations, large cervicofacial infantile hemangiomas, arterial anomalies, aortic coarctation, cardiac abnormalities, and eye abnormalities. The long-term outcome of PHACE syndrome patients is unclear; however, it seems that they are at risk for childhood stroke. The radiologist has an important role on diagnosis of PHACE syndrome and in the assessment of potential complications. Investigation of infants with segmental craniofacial hemangiomas should include cranial magnetic resonance imaging (MRI) and magnetic resonance angiography (MRA) of the cerebral and cervical arteries. Brain MRI and MRA findings of a 5-year-old female patient with PHACE syndrome are presented.
Initiating Opioid Agonist Treatment for Opioid use Disorder Nationally in the Veterans Health Administration: Who Gets What?
Authors: Manhapra Ajay , Stefanovics Elina , Rosenheck Robert 
Published: August 2019

Background Despite substantial benefits associated with opioid agonist treatment (OAT) with buprenorphine and methadone for opioid use disorder (OUD), only a small proportion of patients with OUD initiate OAT. There is a lack of studies addressing the correlates of OAT initiation among patients with OUD. Methods Using Veterans Health Administration (VHA) national administrative data, we identified veterans with OUD who started OAT with either buprenorphine or methadone maintenance treatment (MMT) in fiscal year (FY) 2012 (first prescription of buprenorphine or first methadone clinic visit after the first 60 days of FY) and those who received no OAT that year. Multivariate logistic regression models including sociodemographic characteristics, diagnoses, and service and psychotropic drug use variables were used to identify independent predictors of OAT initiation. Results Greater age (10-year increments; odds ratio [OR]: 0.96, 95% confidence interval [CI]: 0.0.9–0.97) and black race (OR: 0.46, 95% CI: 0.38–0.55) were associated with lower odds of being started on buprenorphine compared with no OAT, but not with MMT initiation. Veterans with cocaine and anxiolytic-sedative hypnotic use disorders had higher odds of being started on both buprenorphine and methadone compared with no OAT. Receipt of any mental health inpatient treatment was associated with higher odds of being started on buprenorphine but not methadone. Overall, we were unable to identify a robust set of patient characteristics associated with initiation of OAT. Conclusion This study points out the stark reality that in the middle of an opioid crisis, we have very little insight into which patients with OUD initiate OAT.
On a definition of the SI second with a set of optical clock transitions
Authors: Lodewyck Jérôme 
Published: August 2019

Abstract
The current SI second based on the atomic hyperfine transition in the ground state of 133Cs is expected to be replaced by a new definition based on optical frequency standards, whose estimated uncertainty has now been established two orders of magnitude lower than the accuracy of the best Cs primary standards. However, such a redefinition of the second is hindered by the fact that many atomic species are potential contenders to become the new primary frequency standard. In this paper, we propose to resolve this issue by defining a composite frequency unit based on the weighted geometric mean of the individual frequencies of different atomic transitions. This unit has the property to be realisable with any single clock whose transition composes the unit, provided that at least a few frequency ratios are available, with an accuracy that marginally differs from the nominal clock uncertainty. We show that the unit can be updated as the performances of the contributing transitions evolve, without incurring a drift on the unit itself.
Analytical Review of the Reports Presented at the 14th International Conference on Films and Coatings (ICFC14)
Authors: Kuznetsov V G , Kostrin D K 
Published: August 2019

Abstract
In this preface main trends in the development of films and coatings technology presented at the 14th International Conference on Films and Coatings (ICFC14) that was held on 14–16 May 2019 in Saint Petersburg, Russia are discussed.
Effect of electrolyte composition on electrochemical formation and properties of ceramic-like coatings on aluminum alloys
Authors: Borisov A M , Krit B L , Lyudin V B , Peretyagin P Yu , Suminov I V , Apelfeld A V , Anikin K A , Kondratsky I O 
Published: August 2019

Abstract
The electrochemical formation of ceramic-like coatings on D16 aluminum alloy was carried out using microarc oxidation method in phosphate (1.5 g/L NaOH + 9 g/L Na2SiO3 + 3 g/L Na3PO4) and molybdate (5 g/L NaOH + 30 g/L Na2SiO3 + 5 g/L (NH4)6Mo7O24) electrolytes. White and black ceramic-like coatings with thickness of 200–300 microns were obtained in phosphate and molybdate electrolytes, respectively.