bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of hepatitis E infection in HIV/HCV-coinfected patients in Spain (2012–2014)
Authors: Vázquez-Morón Sonia , Berenguer Juan , González-García Juan , Jiménez-Sousa Ma Ángeles , Canorea Isabel , Guardiola Josep M. , Crespo Manuel , Quereda Carmen , Sanz José , Carrero Ana , Hontañón Victor , Avellón Ana , Resino Salvador 
Published: February 2019

AbstractHepatitis E virus (HEV) has emerged as a relevant pathogen for HIV-infected patients. However, there is scarce data on HEV infection in HIV/HCV-coinfected individuals with advanced fibrosis, which seems to increase the risk of HEV infection and worsen the prognosis of liver disease. We aimed to determine the prevalence of anti-HEV antibodies, acute hepatitis E, resolved hepatitis E, and exposure to HEV in HIV/HCV-coinfected patients and to evaluate associations with clinical and epidemiological characteristics. We performed a cross-sectional study on 198 HIV/HCV-coinfected patients, 30 healthy controls and 36 HIV-monoinfected patients. We found a low concordance between techniques used for detection of anti-HEV antibodies (ELISA versus Immunoblot), particularly in HIV/HCV-coinfected patients. HIV/HCV-coinfected patients showed the highest prevalence of IgG against HEV, resolved hepatitis E, and exposure to HEV (19.2%, 17.2%, and 22.2% respectively). However, we did not find any samples positive for HEV-RNA nor significant differences between groups. Moreover, HIV/HCV-coinfected patients with CD4 T-cells <350 cells/mm3 had higher prevalence for anti-HEV IgG antibodies, resolved hepatitis E, and exposure to HEV than healthy controls or those with CD4 T-cells ≥ 350 cells/mm3 (p = 0.034, p = 0.035, and p = 0.053; respectively). In conclusion, HIV/HCV-coinfected patients in Spain have a high prevalence for IgG anti-HEV antibodies, resolved hepatitis E, and exposure to HEV; particularly patients with CD4+T-cells <350 cells/mm3.
Development of High-Performance Supercapacitor based on a Novel Controllable Green Synthesis for 3D Nitrogen Doped Graphene
Authors: Elessawy Noha A. , El Nady J. , Wazeer W. , Kashyout A. B. 
Published: February 2019

Abstract3D sponge nitrogen doped graphene (NG) was prepared economically from waste polyethylene-terephthalate (PET) bottles mixed with urea at different temperatures using green approach via a novel one-step method. The effect of temperature and the amount of urea on the formation of NG was investigated. Cyclic voltammetry and impedance spectroscopy measurements, revealed that nitrogen fixation, which affects the structure and morphology of prepared materials improve the charge propagation and ion diffusion. The prepared materials show outstanding performance as a supercapacitor electrode material, with the specific capacitance going up to 405 F g−1 at 1 A g−1. An energy density of 68.1 W h kg−1 and a high maximum power density of 558.5 W kg−1 in 6 M KOH electrolytes were recorded for the optimum sample. The NG samples showed an appropriate cyclic stability with capacitance retention of 87.7% after 5000 cycles at 4 A g−1 with high charge/discharge duration. Thus, the prepared NG herein is considered to be promising, cheap material used in energy storage applications and the method used is cost-effective and environmentally friendly method for mass production of NG in addition to opening up opportunities to process waste materials for a wide range of applications.
Structure and function of the Ts2631 endolysin of Thermus scotoductus phage vB_Tsc2631 with unique N-terminal extension used for peptidoglycan binding
Authors: Plotka Magdalena , Sancho-Vaello Enea , Dorawa Sebastian , Kaczorowska Anna-Karina , Kozlowski Lukasz P. , Kaczorowski Tadeusz , Zeth Kornelius 
Published: February 2019

AbstractTo escape from hosts after completing their life cycle, bacteriophages often use endolysins, which degrade bacterial peptidoglycan. While mesophilic phages have been extensively studied, their thermophilic counterparts are not well characterized. Here, we present a detailed analysis of the structure and function of Ts2631 endolysin from thermophilic phage vB_Tsc2631, which is a zinc-dependent amidase. The active site of Ts2631 consists of His30, Tyr58, His131 and Cys139, which are involved in Zn2+ coordination and catalysis. We found that the active site residues are necessary for lysis yet not crucial for peptidoglycan binding. To elucidate residues involved in the enzyme interaction with peptidoglycan, we tested single-residue substitution variants and identified Tyr60 and Lys70 as essential residues. Moreover, substitution of Cys80, abrogating disulfide bridge formation, inactivates Ts2631, as do substitutions of His31, Thr32 and Asn85 residues. The endolysin contains a positively charged N-terminal extension of 20 residues that can protrude from the remainder of the enzyme and is crucial for peptidoglycan binding. We show that the deletion of 20 residues from the N-terminus abolished the bacteriolytic activity of the enzyme. Because Ts2631 exhibits intrinsic antibacterial activity and unusual thermal stability, it is perfectly suited as a scaffold for the development of antimicrobial agents.
Mind-wandering, or the allocation of attentional resources, is sleep-driven across childhood
Authors: Spruyt Karen , Herbillon Vania , Putois Benjamin , Franco Patricia , Lachaux Jean-Philippe 
Published: February 2019

AbstractMind-wandering or the spontaneous, uncontrolled changes in the allocation of attention resources (lapses) may cause variability in performance. In childhood, the relationship between the activation state of the brain, such as in attentional performance, and sleep has not been explored in detail. We investigated the role of sleep in attentional performance, and explored the most important parameters of their relationship. We objectively measured momentary lapses of attention of 522 children and correlated them with sleep schedules. In the subgroup of young children (age 7.1 ± 0.6 years; 60.8% girls), increasing age, long sleep duration and assessment closer to the previous night’s sleep period was associated with impaired performance speed and consistency. From pre-adolescence (age 9.4 ± 0.8 years; 50.5% girls) onwards somno-typologies may develop. As a result, in adolescence (age 13.4 ± 1.2 years; 51.3% girls) not only sleep duration but also sleep midpoint and sleep regularity influence the individual speed and stability of attention. Across development, regularity of sleep, individual sleep midpoint and bedtime become increasingly important for optimal performance throughout the day. Attentional performance and sleep shared almost half of their variance, and performance was sleep-driven across childhood. Future studies should focus on intra- and inter-individual differences in sleep-wake behavior to improve performance or decrease mind-wandering in youth by targeting sleep habits.
Effect of Bi-functional Hierarchical Flower-like CoS Nanostructure on its Interfacial Charge Transport Kinetics, Magnetic and Electrochemical Behaviors for Supercapacitor and DSSC Applications
Authors: Ashok Kumar K , Pandurangan A , Arumugam S , Sathiskumar M 
Published: February 2019

AbstractMetal sulfides are of great interest for future electrode materials in supercapacitor and solar cell applications owing to their superior electrochemical activity and excellent electrical conductivity. With this scope, a binary transition metal sulfide (CoS) is prepared via one-step hydrothermal synthesis. Hexagonal phase of CoS with space group of P63/mmc(194) is confirmed by XRD analysis. Additional cubic Co3S4 phase in the prepared sample originates the mixed valence state of Co (Co2+ and Co3+) is affirmed from XPS analysis. Morphological features are visualized using HRSEM images that shows nanoflower shaped star-anise structure. Employing the prepared CoS as active electrode material, interfacial charge transport kinetics is examined by EIS-Nyquist plot. The supercapacitive performances are tested in two and three-electrode system which exhibited respective specific capacitances of 57 F/g and 348 F/g for 1 A/g. Further, the fabricated asymmetric CoS//AC supercapacitor device delivers an appreciable energy density of 15.58 Wh/kg and power density of 700.12 W/kg with excellent cyclic stability of 97.9% and Coulombic efficiency of 95% over 2000 charge-discharge cycles. In addition, dye-sensitized solar cells are fabricated with CoS counter electrode and the obtained power conversion efficiency of 5.7% is comparable with standard platinum based counter electrode (6.45%). Curie-Weiss plot confirms the transition of paramagnetic nature into ferrimagnetic behavior at 85 K and Pauli-paramagnetic nature at 20 K respectively. Temperature dependent resistivity plot affirms the metallic nature of CoS sample till 20 K and transition to semiconducting nature occurs at <20 K owing to Peierl’s transition effect.
Mycobacterium tuberculosis Rv0366c-Rv0367c encodes a non-canonical PezAT-like toxin-antitoxin pair
Authors: Tandon Himani , Sharma Arun , Sandhya Sankaran , Srinivasan Narayanaswamy , Singh Ramandeep 
Published: February 2019

AbstractToxin-antitoxin (TA) systems are ubiquitously existing addiction modules with essential roles in bacterial persistence and virulence. The genome of Mycobacterium tuberculosis encodes approximately 79 TA systems. Through computational and experimental investigations, we report for the first time that Rv0366c-Rv0367c is a non-canonical PezAT-like toxin-antitoxin system in M. tuberculosis. Homology searches with known PezT homologues revealed that residues implicated in nucleotide, antitoxin-binding and catalysis are conserved in Rv0366c. Unlike canonical PezA antitoxins, the N-terminal of Rv0367c is predicted to adopt the ribbon-helix-helix (RHH) motif for deoxyribonucleic acid (DNA) recognition. Further, the modelled complex predicts that the interactions between PezT and PezA involve conserved residues. We performed a large-scale search in sequences encoded in 101 mycobacterial and 4500 prokaryotic genomes and show that such an atypical PezAT organization is conserved in 20 other mycobacterial organisms and in families of class Actinobacteria. We also demonstrate that overexpression of Rv0366c induces bacteriostasis and this growth defect could be restored upon co-expression of cognate antitoxin, Rv0367c. Further, we also observed that inducible expression of Rv0366c in Mycobacterium smegmatis results in decreased cell-length and enhanced tolerance against a front-line tuberculosis (TB) drug, ethambutol. Taken together, we have identified and functionally characterized a novel non-canonical TA system from M. tuberculosis.
Schizophrenia risk factors in exceptional achievers: a re-analysis of a 60-year-old database
Authors: Szöke Andrei , Pignon Baptiste , Schürhoff Franck 
Published: February 2019

AbstractCurrent medical research has focused on diseases and their associated risk factors. As such, these factors are assumed to have a deleterious effect. An alternative hypothesis is that some of these risk factors would also increase the chance for an opposite, positive outcome. To test this hypothesis, we considered exceptional social achievement and schizophrenia as opposite outcomes. Sixty years ago, researchers in France collected data on socio-demographic factors associated with exceptional social achievement. As the number of female subjects in the original database was very limited, we restricted our analyses to men. We tested the odds of achieving prominence in the presence of factors known to be associated with an increased risk of schizophrenia, namely migration, urbanicity, seasonality of birth, birth order, and paternal age. Three of the five factors tested significantly increased the odds for exceptional social achievement (urban birth, being the first-born and father’s age over 35). Our findings suggest that some of the factors that are currently considered as risk factors for schizophrenia could diversifying factors. Widening the focus of research to include all potential effects of factors associated with disease could have important consequences on our understanding of causal mechanisms and for designing public health interventions.
Rectangular plasmonic interferometer for high sensitive glycerol sensor
Authors: Khajemiri Zahra , Lee Dukhyung , Hamidi Seyedeh Mehri , Kim Dai-Sik 
Published: February 2019

AbstractA novel plasmonic interferometric sensor intended for application to biochemical sensing has been investigated experimentally and theoretically. The sensor was included a slit surrounded by rectangular grooves using a thick gold film. A three-dimensional finite difference time-domain commercial software package was applied to simulate the structure. The Focused ion beam milling has been used as a mean to fabricate series of rectangular plasmonic interferometer with varying slit-groove distance L. Oscillation behavior is shown by transmission spectra in a broadband wavelength range between 400 nm and 800 nm in the distance between slit and grooves. Red-shifted interference spectrum is the result of increasing refractive indices. The proposed structure is functional from visible to near-infrared wavelength range and yields a sensitivity of 4923 nm/RIU and a figure of merit as high as 214 at 729 nm wavelength. In conclusion, this study indicates the possibility of fabricating a low cost, compact, and real-time high-throughput plasmonic interferometer.
Combining MALDI-TOF and genomics in the study of methicillin resistant and multidrug resistant Staphylococcus pseudintermedius in New Zealand
Authors: Nisa Shahista , Bercker Clément , Midwinter Anne C. , Bruce Ian , Graham Chris F. , Venter Pierre , Bell Allan , French Nigel P. , Benschop Jackie , Bailey Karen M. , Wilkinson David A. 
Published: February 2019

AbstractStaphylococcus pseudintermedius is an opportunistic and emerging zoonotic pathogen that primarily colonises the skin of dogs. Many common variants are methicillin resistant (MRSP) or multidrug resistant (MDR), and drug resistance is increasingly reported across the globe. In New Zealand, MRSP isolation remains rare in clinics. To pre-emptively inform diagnostic and antimicrobial stewardship practices, we examine isolates of S. pseudintermedius, MRSP and MDR-MRSP from New Zealand dogs using a combination of methodologies. Genetic and genomic data combined with antimicrobial susceptibility screening identify common drug-resistance profiles and their genetic determinants. We demonstrate that sensitive and specific species-level identification of S. pseudintermedius can be achieved using Bruker MALDI-TOF MS and, further, that this technique can be used to identify some common subtype variants, providing a level of categorical precision that falls somewhere between single-locus and multi-locus sequence typing. Comparative genomics analysis of global S. pseudintermedius data shows that MRSP moves frequently across the globe, but that horizontal gene transfer events resulting in the acquisition of the SCCmec cassette (responsible for beta-lactam antibiotic resistance) are infrequent. This suggests that biosecurity and surveillance in addition to antibiotic stewardship should play important roles in mitigating the risk of MRSP, especially in countries such as New Zealand where MRSP is still rare.
Influences of scan-position on clinical ultra-high-resolution CT scanning: a preliminary study
Authors: Li Lu , Li HuiMin , Shu JinEr , Pan JiangFeng , Chen XiaoRong , Ying MingLiang , Xu YiBin , Wang Dingjun , Pang Peipei 
Published: February 2019

AbstractThe aim of this study is to access influences of scan-position on clinical ultra-high-resolution CT scanning. We proposed a breath-hold assisted ultra-high-resolution scanning technology (scan scheme G) and compared with scan scheme A (regular CT plain scan) and scheme B (1024 ultra-high-resolution scan with patients stay in supine position). A total of 30 patients with fGGO were included in this study. Three highly experienced chest imaging doctors were employed to score the image and to select regions of interest (ROIs) for CT value and signal-to-noise ratio (SNR) calculation. In comparison with scan A and B, this new scan scheme G shows more clear CT images and higher SNRs at overall lung field (the p-values of A versus G and B versus G are 0.041 and 0.065, respectively). These findings suggest that scan-G provides a better image quality and contributes significantly to clinical detection accuracy of fGGO.
Baduanjin exercise intervention for community adults at risk of ischamic stroke: A randomized controlled trial
Authors: Zheng Guohua , Chen Bai , Fang Qianying , Lin Qiu , Tao Jing , Chen Lidian 
Published: February 2019

AbstractThe aim of current study was to assess the effects of Baduanjin exercise on cerebrovascular function, cardiac structure and cardiac function, static pulmonary function, traditional risk factors of CVD and the related psychological outcomes in older community adults at risk for ischaemic stroke. A randomized controlled trial was conducted in three community between November 2013 and October 2015. Older community-dwelling adults (N = 170) were randomly allocated into either a Baduanjin training (5 × 60 min/weekly) or control group who kept their unaltered lifestyle during a 12-week intervention period. Primary (cerebral haemodynamic parameters) and secondary outcomes (cardiac structure, cardiac function, static pulmonary function, traditional risk factors and the related psychological outcomes) were measured at baseline, after a 12-week intervention period and after an additional 12-week follow-up period. After the 12-week intervention period and additional 12-week follow-up period, the Baduanjin exercise group displayed significant changes in most cerebral haemodynamic parameters compared to the control group: lower systolic blood pressure, diastolic blood pressure, plasma total cholesterol levels, waist circumference, hip circumference and waist/hip ratio; and improved mood, self-confidence, self-esteem, quality of life and sleep quality. A supervised 12-week Baduanjin exercise intervention was effective and safe in modulating cerebral haemodynamics, reducing blood pressure and improving anthropometric parameters and related psychological outcomes in older community adults at risk for ischaemic stroke.
Evolutionary history and postglacial colonization of an Asian pit viper (Gloydius halys caucasicus) into Transcaucasia revealed by phylogenetic and phylogeographic analyses
Authors: Asadi Atefeh , Montgelard Claudine , Nazarizadeh Masoud , Moghaddasi Akram , Fatemizadeh Faezeh , Simonov Evgeniy , Kami Haji Gholi , Kaboli Mohammad 
Published: February 2019

AbstractIt has been generally acknowledged that glacial climates at the time of the Pleistocene altered the patterns of species distributions, prompting latitudinal and altitudinal distribution shifts in several species, including poikilothermic species commonly known for their thermal sensitivity. However, the historical phylogeographic patterns of such species have remained largely unknown. Here, we present the historical biogeographic, phylogenetic, and phylogeographic relationships of the Caucasian pit viper, G. h. caucasicus, based on two mtDNA (cyt b and ND4) and one nDNA (c-mos) genes. This pit viper represents the westernmost member of the Crotalinae subfamily in the Palearctic and occurs in a variety of habitats, from 30 m to 3,000 m above sea level. In Iran, it is distributed on the northern and southern slopes of the Alborz Mountains, rendering it a target for phylogenetic and phylogeographic studies of a terrestrial poikilothermic animal. Our study identified four Iranian lineages of G. h. caucasicus along the northeastern to northwestern slopes of the Alborz Mountains and southern Azerbaijan (Talysh Mountains). Diversification of the Iranian lineages highlights population expansion and subsequent isolation into four plausible refugial areas during the Quaternary paleo-climatic oscillations, confirmed by our molecular dating and historical biogeographic analyses. The results of coalescence-based simulations support the incursion of the species from northeastern Iran to the western end of the Alborz, and then toward Transcaucasia via two directions: northern and southern slopes of the Alborz Mountains. Furthermore, our results clearly implied that G. h. caucasicus should be elevated to species rank and further referred to as G. caucasicus (Nikolsky, 1916).
Statistical analysis of timeseries data reveals changes in 3D segmental coordination of balance in response to prosthetic ankle power on ramps
Authors: Pickle Nathaniel T. , Silverman Anne K. , Wilken Jason M. , Fey Nicholas P. 
Published: February 2019

AbstractActive ankle-foot prostheses generate mechanical power during the push-off phase of gait, which can offer advantages over passive prostheses. However, these benefits manifest primarily in joint kinetics (e.g., joint work) and energetics (e.g., metabolic cost) rather than balance (whole-body angular momentum, H), and are typically constrained to push-off. The purpose of this study was to analyze differences between active and passive prostheses and non-amputees in coordination of balance throughout gait on ramps. We used Statistical Parametric Mapping (SPM) to analyze time-series contributions of body segments (arms, legs, trunk) to three-dimensional H on uphill, downhill, and level grades. The trunk and prosthetic-side leg contributions to H at toe-off when using the active prosthesis were more similar to non-amputees compared to using a passive prosthesis. However, using either a passive or active prosthesis was different compared to non-amputees in trunk contributions to sagittal-plane H during mid-stance and transverse-plane H at toe-off. The intact side of the body was unaffected by prosthesis type. In contrast to clinical balance assessments (e.g., single-leg standing, functional reach), our analysis identifies significant changes in the mechanics of segmental coordination of balance during specific portions of the gait cycle, providing valuable biofeedback for targeted gait retraining.
Deactivation of default mode network during touch
Authors: Strauss Timmy , Kämpe Robin , Hamilton J. Paul , Olausson Hakan , Rottstädt Fabian , Raue Claudia , Croy Ilona 
Published: February 2019

AbstractInterpersonal touch possesses a strong affective component, which immediately evokes attention. The neural processing of such touch is moderated by specialized C-tactile nerve fibers in the periphery and results in central activation of somatosensory areas as well as regions involved in social processing, such as the superior temporal gyrus (STG). In the present functional neuroimaging investigation, we tested the hypothesis that the attention grasping effect of interpersonal touch as compared to impersonal touch is reflected in a more-pronounced deactivation of the default mode network (DMN). Using functional magnetic resonance imaging, we investigated the neural processing of interpersonal relative to impersonal touch conditions that were furthermore modulated by stroking velocity in order to target c-tactile nerve fibers to a different extent. A sample of 30 healthy participants (19 women, mean age 40.5 years) was investigated. In the impersonal touch, participants were stroked with a brush on the forearm. In the interpersonal touch condition, the experimenter performed the stroking with the palm of his hand. Interpersonal touch was rated as more pleasant and intense than impersonal touch and led to a stronger blood oxygen level dependent (BOLD) signal increase in the somatosensory cortex SII extending to the superior temporal cortex. Over all touch conditions, this activation was coupled in time to the deactivation of prominent nodes of the DMN. Although deactivation of the DMN was most pronounced for interpersonal touch conditions, the direct comparison did not show significant differences in DMN deactivation between interpersonal and impersonal touch or between different stroking velocities. We therefore conclude that all applied touch conditions deactivate the DMN and the strong connection to areas which code the contextual and social characteristics of affective touch may explain the attention grasping effect of touch.