bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of GOCI-II satellite remote sensing products in Lake Taihu
Authors: Zhao Min , Li Huaming , Li Hao , Zhang Xuan , Ding Xiaosong , Gong Fang 
Published: November 2024

IntroductionThe Geostationary Ocean Color Imager-II (GOCI-II), launched on February 19, 2020, offers increased observation times throughout the day and higher spatial resolution compared to its predecessor, the Geostationary Ocean Color Imager (GOCI), launched in 2010. To ensure the reliability of GOCI-II data for practical applications, the accuracy of remote sensing products needs to be validated. This study uses in situ data from Lake Taihu for validation.MethodsWe assessed the accuracy of GOCI-II remote sensing products, including remote sensing reflectance derived using two atmospheric correction algorithms: ultraviolet (UV) and near-infrared (NIR). The study also evaluated the accuracy of derived parameters, such as chlorophyll-a (Chl-a) concentration, total suspended matter (TSM) concentration, and phytoplankton absorption coefficient (aph), based on these atmospheric correction algorithms. In situ measurements from Lake Taihu were used as ground truth data for validation.ResultsOur results revealed that the UV atmospheric correction algorithm provided higher accuracy in Lake Taihu compared to the NIR algorithm. The average absolute percentage deviations (APDs) for remote sensing reflectance across different bands were: 25.17% (412 nm), 29.69% (443 nm), 22.27% (490 nm), 19.38% (555 nm), 36.83% (660 nm), and 33.0% (680 nm). Compared to NIR-derived products, the UV algorithm showed improved accuracy for Chl-a concentration, TSM concentration, and aph, with reductions in APD values by 16.92%, 3.32%, and 10.91%, respectively. When applying UV correction, the 412 nm band performed better than the 380 nm band, likely due to a lower signal-to-noise ratio at 380 nm and smaller extrapolation errors at 412 nm.DiscussionWhile the NIR algorithm is suitable for open ocean waters, the UV algorithm demonstrated higher accuracy in turbid environments such as Lake Taihu. Therefore, a combined UV-NIR atmospheric correction algorithm may be more effective for handling various types of water environments. Additionally, further research is needed to develop more suitable retrieval algorithms for Chl-a concentration and aph in eutrophic waters to improve accuracy.
Vitamin K intake levels are associated with bone health in people aged over 50 years: a NHANES-based survey
Authors: Guo Jiankui , Zhou Ziqi , Gong Jie , Hu Wen , Liu Yuan 
Published: November 2024

BackgroundBone health is important for older adults, and vitamin K (VK) is central to regulating bone formation and promoting bone health. However, whether VK can reduce the risk of osteoporosis and bone loss is unclear. This study hypothesized that different levels of VK intake exert varying effects on bone health in people aged over 50 years.MethodsIndividuals aged above 50 years were recruited from the National Health and Nutrition Examination Survey. VK intake, based on 24-h dietary recall, was divided into three groups, namely the high, medium, and low groups, by sex and tertile. Weighted multiple logistic regression was used to investigate the effects of VK intake on the risk of osteoporosis and bone loss at the femoral neck, trochanter, intertrochanter, total femur, lumbar spine, and overall.ResultsThis study included 5,075 individuals. Of them, 1,001 (18%) had osteoporosis (808 women, 83%) and 2,226 (46%) had osteopenia (1,076 women, 54%). Overall, a medium level of VK intake was associated with a reduced risk of bone loss. In women, medium- [odds ratio, OR (95% confidence interval, CI): 0.66(0.47, 0.93)] and high-level [OR (95% CI): 0.71(0.52, 0.98)] VK intake were associated with a decreased risk of osteoporosis. In contrast, only medium-level VK intake was associated with a reduced risk of bone loss [OR (95% CI): 0.58(0.41, 0.81)]. Similar results were obtained for the trochanter, intertrochanter, total femur, and lumbar spine. In men, only medium-level VK intake was associated with a reduced risk of bone loss at the femoral neck [OR (95% CI): 0.66(0.48, 0.90)], whereas high-level VK intake corresponded to a reduced risk of bone loss to the lumbar spine [OR (95% CI): 0.68(0.47, 0.99)]. Nonetheless, VK intake levels did not affect the risk of osteoporosis.ConclusionThis study demonstrates sex- and bone-site-specific variations in the associations between VK intake levels and bone health in individuals aged over 50 years. Further large-scale cohort studies or randomized controlled trials are warranted to explore the effects of different VK intake levels on bone health in people regardless of their sex and bone site.
Influence of specific tobacco endophytic Bacillus on tobacco leaf quality enhancement during fermentation
Authors: Wei Jinbin , Song Kai , Zang Zhipeng , Yang Hongjing , Gao Yuzhen , Zhang Jiandong , Wang Zhen , Liu Chen 
Published: November 2024

IntroductionThis study aimed to investigate the potential role of endophytic bacteria in tobacco leaves during the fermentation process to enhance the quality of tobacco.MethodsWe isolated 11 endophytic bacteria from fresh tobacco leaves and selected Bacillus halotolerans NS36 and Bacillus mycoides NS75 based on sensory evaluation, both of which significantly improved the sensory quality of tobacco leaves.ResultsSpecifically, NS36 decreased offensive taste in tobacco leaves, while NS75 improved the quality by increasing the aroma. Chemical analysis revealed that fermentation with B. halotolerans NS36 significantly decreased the content of irritant compounds such as lignin, cellulose, starch, and pectin. In contrast, fermentation with B. mycoides NS75 reduced the content of cellulose, starch, and protein, while significantly increasing the content of Amadori compounds and glycosides. Through whole-genome sequencing, we predicted enzyme systems related to these chemical changes. B. halotolerans NS36 mainly secreted enzyme systems associated with the degradation of lignin, cellulose, starch, and pectin, thereby reducing irritants in tobacco leaves, diminishing unpleasant tastes, and achieving a more balanced sensory quality. B. mycoides NS75, on the other hand, secreted enzyme systems related to protein and glycoside hydrolysis, increasing Maillard reaction products and glycosylated compounds in tobacco leaves, thus enhancing the aroma quality and quantity.DiscussionThe findings of this study offer a new perspective for the tobacco industry, namely, the use of endophytic bacilli to improve the off-flavors and aroma of tobacco leaves, which could not only enhance the industrial applicability of tobacco leaves but also potentially strengthen the market competitiveness of products. These discoveries lay the foundation for further research and application, especially in the development of new biotechnologies to improve the quality of tobacco products.
Body mass index and fat influences the role of Bifidobacterium genus in lupus patients concerning fibrinogen levels
Authors: Chero-Sandoval Lourdes , Higuera-Gómez Andrea , Cuevas-Sierra Amanda , de Cuevillas Begoña , Castejón Raquel , Martínez-Urbistondo María , Mellor-Pita Susana , Moreno-Torres Víctor , de Luis Daniel , Martínez J. Alfredo 
Published: November 2024

IntroductionMetabolic disorders and autoimmune diseases elicit distinct yet interconnected manifestations of inflammation, which may be boosted by an excess of body adiposity. The purpose of this investigation was to analyze anthropometric, biochemical, and inflammatory/coagulation variables concerning patients diagnosed with systemic lupus erythematosus (SLE) exploiting low-grade metabolic inflammation (MI), as reference.MethodsA population stratification by body mass index (BMI), allowed to assess the impact of adiposity on the putative role of gut microbiota composition on coagulation markers. A total of 127 participants with MI and SLE were categorized into two main groups based on their BMI, following WHO criteria: a low BMI group (&lt;30 kg/m2) and a high BMI group (≥30 kg/m2). Each group included recorded data on demographics, comorbidities, and key clinical markers. Anthropometric and body composition variables, clinical features, and inflammatory/coagulation markers were measured while fecal 16S rRNA sequencing was examined at the genus Bifidobacterium. Regression models were fitted to evaluate the relationship between gut microbiota, inflammatory/coagulation markers, and body weight in these types of diseases.ResultsThe study revealed worse clinical outcomes in anthropometric, body composition, and clinical markers in low-grade MI conditions as compared to SLE. However, inflammatory and coagulation markers such as C-reactive protein (CRP) and fibrinogen were significantly more elevated in patients with SLE, which was exacerbated by high BMI/ body fat as compared to the other screened groups. An interaction analysis revealed that fibrinogen levels showed different trends when Bifidobacterium was increased depending on BMI/adiposity, which evidenced an effect modification by this microorganism in patients with SLE.DiscussionThese findings underline that gut microbiota composition, particularly the presence of Bifidobacterium, may play a crucial role in modulating inflammation and coagulation processes in patients with SLE and high fat. These insights highlight the potential of targeting gut microbiota as a therapeutic strategy to mitigate inflammation and improve clinical outcomes in SLE patients.
Associations of motor and neuropsychiatric symptoms with comorbidities in prodromal Parkinson’s disease
Authors: Chen Jia-Ru , Sun Yan , Ma Yu-Ju , Tan Lan 
Published: November 2024

ObjectiveTo investigate the associations between comorbidities and multimorbidity patterns with motor and neuropsychiatric symptoms in patients with Parkinson’s disease (PD) in prodromal PD.MethodsMultimorbidity is defined as the coexistence of two or more long-term conditions (LTCs) (also known as multiple comorbidities). A total of 921 participants without PD were included in the Parkinson’s Progression Markers Initiative (PPMI) database and were categorized according to the LTC count. Participants were evaluated on motor and psychiatric symptoms. Pearson correlation to examine relationship of comorbidities and target symptoms. The baseline population was analyzed using Multiple linear regression model, while mixed effects model was utilized for longitudinal analysis. Fuzzy C-means clustering analysis was conducted to identify comorbidity patterns, followed by multiple linear regression for further analysis.ResultsAt baseline, a higher LTC count was significantly correlated with more severe motor (MDS-UPDRS I, II, ADL, all P &lt; 0.05) and neuropsychiatric symptoms (QUIP, P &lt; 0.001). Three multimorbidity patterns were identified. Among them, the cardiometabolic multimorbidity pattern (CAR) had the most significant correlation with the aforementioned symptoms. Our longitudinal analysis indicated that an increase in the LTC count was associated with the exacerbation of motor and neuropsychiatric symptoms.ConclusionComorbidities were cross-sectionally and longitudinally associated with the motor and neuropsychiatric symptoms of patients with prodromal PD. Among the three multimorbidity patterns, CAR posed the highest threat to the risk of more severe motor and neuropsychiatric symptoms.
Restoration of natural somatic sensations to the amputees: finding the right combination of neurostimulation methods
Authors: Soghoyan Gurgen , Biktimirov Artur R. , Piliugin Nikita S. , Matvienko Yury , Kaplan Alexander Y. , Sintsov Mikhail Y. , Lebedev Mikhail A. 
Published: November 2024

Limb amputation results in such devastating consequences as loss of motor and sensory functions and phantom limb pain (PLP). Neurostimulation-based approaches have been developed to treat this condition, which provide artificial somatosensory feedback such as peripheral nerve stimulation (PNS), spinal cord stimulation (SCS), and transcutaneous electrical nerve stimulation (TENS). Yet, the effectiveness of different neurostimulation methods has been rarely tested in the same participants. Meanwhile, such tests would help to select the most effective method or a combination of methods and could contribute to the development of multisensory limb prostheses. In this study, two transhumeral amputees were implanted with stimulating electrodes placed in the medial nerve and over the spinal cord epidurally. PNS and SCS were tested in each participant as approaches to enable tactile and proprioceptive sensations and suppress PLP. Both PNS and SCS induced sensation in different parts of the phantom hand, which correlated with cortical responses detected with electroencephalographic (EEG) recordings. The sensations produced by PNS more often felt natural compared to those produced by SCS. Еvoked response potentials (ERPs) were more lateralized and adapted faster for PNS compared to SCS. In the tasks performed with the bionic hand, neurostimulation-induced sensations enabled discrimination of object size. As the participants practiced with neurostimulation, they improved on the object-size discrimination task and their sensations became more natural. А combination of PNS and TENS enabled sensations that utilized both tactile and proprioceptive information. This combination was effective to convey the perception of object softness. In addition to enabling sensations, neurostimulation led to a decrease in PLP.Clinical trial registrationhttps://clinicaltrials.gov/, identifier, #NCT05650931.
Semen Cuscutae flavonoids activated the cAMP-PKA-CREB-BDNF pathway and exerted an antidepressant effect in mice
Authors: Shao Qianfeng , Li Yue , Jin Lin , Zhou Sheng , Fu Xiaowei , Liu Tong , Luo Guangbin , Du Shaohui , Chen Che 
Published: November 2024

BackgroundSemen Cuscutae flavonoids (SCFs) constitute a class of metabolites of Semen Cuscutae, a botanical drug that was recently found to have an anti-depression effect. This study aimed to evaluate the anti-depression effects of SCFs in chronic unpredictable mild stress (CUMS)-induced mice and to interrogate the underlying mechanisms.Materials and methodsThe CUMS mice were used for assessing the effects of SCFs treatments on depression. Mice were randomly divided into five groups. Four groups were subjected to the CUMS induction and concomitantly administered orally with either the vehicle or with a high-, medium-, and low-dose of SCFs, once per day for 4 weeks. One group was kept untreated as a control. The mice were then assessed for their statuses of a number of depression-related parameters, including body weight, food intake, sucrose preference test (SPT), open field test (OFT), tail suspension test (TST), and forced swim test (FST). In addition, a day after the completion of these tests, biopsies from the hippocampus were harvested and used to perform metabolomics by HPLC-MS/MS and to assess the levels of cAMP by ELISA and the levels of PKA, CREB, p-CREB, and BDNF by Western blot analyses.ResultsSCFs resulted in significant increases in both body weight and food intake and in the amelioration of the depressive-like behaviors in CUMS mice. A high-dose SCFs treatment led to significant alterations in 72 metabolites, of which 26 were identified as potential biomarkers for the SCFs treatment. These metabolites are associated with lipid, amino acid, and nucleotide metabolism. Among 26 metabolites, cAMP was positively correlated with body weight, SPT, OFT-total distance, and OFT-central residence time, while negatively correlated with immobility time in TST and FST, linking a change in cAMP with the SCFs treatment and the significant improvement in depressive symptoms in CUMS mice. Further analyses revealed that the levels of cAMP, PKA, CREB, p-CREB, and BDNF were reduced in the hippocampus of CUMS mice but were all increased following the SCFs treatments.ConclusionSCFs could ameliorate hippocampal metabolic disturbances and depressive behaviors and cause the activation of the cAMP-PKA-CREB-BDNF signaling pathway in the hippocampus of CUMS mice.
Comparing commercial pharmacogenetic testing results and recommendations for antidepressants with established CPIC guidelines
Authors: Nguyen Tiffany T. , Leary Emili J. W. , Lee Joshua T. , Shukla Sanjay K. , Griesbach Sara A. 
Published: November 2024

IntroductionIncreasingly, pharmacogenetic testing helps providers with medication selection based upon patient-specific DNA results. While several government-funded organizations work towards consensus and standardization for testing and interpretation, compliance to these best practices remains inconsistent. Pharmacogenetic testing companies often develop proprietary practices for interpreting and reporting, which can lead to incongruency of reported results among companies and potential discrepancies in interpretation.MethodsTo identify the differences of commercial pharmacogenetic testing vendors’ interpretation of genotype-to-phenotype translations and medication recommendations from the Clinical Pharmacogenetic Implementation Consortium (CPIC) guidelines, a retrospective manual chart review was completed in a large rural healthcare system that utilizes two institution-approved pharmacogenetic vendors. One hundred patients were evaluated: 50 who completed testing through Company A and 50 who completed testing through Company B. Genes of interest for genotype-to-phenotype translation included CYP2B6, CYP2C19, and CYP2D6. Comparison of medication recommendations for drug-gene pairs sertraline (CYP2B6 and/or CYP2C19), escitalopram (CYP2C19), and paroxetine (CYP2D6) were compared with recommendations from CPIC, with consideration of the CPIC Serotonin Reuptake Inhibitor Antidepressants (SSRI) guideline 2023 update. This was accomplished via a novel binning process to enable comparison of company-provided binned medication recommendations with CPIC guideline recommendations. Briefly, the binning system included three categorizations based upon the relevant CPIC guideline recommendations–no action needed (green), recommend monitoring (yellow) and therapeutic intervention or alternative recommended (red).ResultsThere were 32/250 (12.8%) genotype-to-phenotype translation discrepancies from CPIC guidelines, all from Company A. Of 266 evaluated binned medication recommendations, there were 114 (42.9%) discrepancies between the pharmacogenetic testing companies (Company A: 93 discrepancies, Company B: 21 discrepancies) and CPIC’s guideline based upon comparison with the novel binning system.DiscussionSignificant differences were observed between testing companies’ interpretations and recommendations, which is concerning as these discrepancies could lead to providers making medication decisions that are not supported by CPIC’s clinical practice guidelines. This may result in suboptimal outcomes for patients, leading to patient and provider dissatisfaction and erosion of trust with pharmacogenetic testing. A proposed resolution for the discrepancies in company-to-company interpretation is adherence to the CPIC guidelines and transparency in interpretation practices.
Assessing real-world safety of plecanatide: a pharmacovigilance study based on the FDA adverse event reporting system
Authors: Zhang Zhiyuan , Yao Yifan , Zhu Li 
Published: November 2024

BackgroundPlecanatide is a selective gastrointestinal peptide used for the treatment of chronic idiopathic constipation (CIC) and irritable bowel syndrome with constipation (IBS-C). Given its widespread use, understanding the long-term safety of plecanatide in real-world settings is essential.MethodsData for this study were sourced from the United States Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS) from the first quarter of 2017 to the second quarter of 2024. Disproportionality analysis methods were employed to quantify adverse event signals associated with plecanatide. Additionally, a Weibull distribution analysis was conducted to assess changes in adverse events over time. Sensitivity analyses were performed to enhance the robustness of the findings.ResultsA total of 861 cases associated with plecanatide were identified, including 2057 adverse event reports. Common positive adverse events included diarrhea, constipation, abdominal distension, dissatisfaction with treatment, rectal tenesmus, increased fecal volume, abnormal gastrointestinal sounds, and gastrointestinal motility disorders. The majority of adverse events related to plecanatide occurred within the first 7 days of treatment. Findings were consistent across sensitivity analyses.ConclusionThis study preliminarily explores the safety of plecanatide in real-world applications, revealing significant new adverse event signals. These findings provide important safety references for clinicians prescribing plecanatide for CIC and IBS-C.
The impact of flywheel resistance squat training on lower limb strength in female college basketball players
Authors: Xie Lin , Qu Wenhao , Dai Jing , Xu Jiamin , Zhang Wenfeng , Sun Jian , Song Wenfeng , Li Duanying 
Published: November 2024

ObjectiveThis study compared the effects of Flywheel Resistance Squat Training (FRST) vs. Traditional Resistance Squat Training (TRST) on lower body strength in female collegiate basketball players.MethodsNineteen participants were randomly assigned to either the FRST group (n = 9) or the TRST group (n = 10) through a random number draw. Both groups underwent a 6-week intervention with training sessions conducted twice a week. The FRST group utilized an inertia of 0.075 kg ·m2, while another group trained at 80% of their one-repetition maximum (1RM). Each training session consisted of 4 sets of 8 repetitions with a 3-minute rest between sets. Both groups performed standardized warm-ups and stretches before and after each training session. The effectiveness of the training methods was assessed through the Countermovement Jump (CMJ), Reactive Strength Index (RSI), Eccentric Utilization Ratio, Running Vertical Jump, and 1RM squat.ResultsThe FRST and TRST groups showed differences within groups in both CMJ and 1RM squat (p &lt; 0.01), with the FRST group demonstrating moderate effect sizes in CMJ (Hedges’ g = 0.59) and 1RM (Hedges’ g = 1.01). However, there were no differences between groups (p &gt; 0.05). The Eccentric Utilization Ratio showed a small effect size (p = 0.78; ηp2=0.01). Additionally, Reactive Strength Index and Running Vertical Jump exhibited low test-retest reliability.ConclusionThe two groups did not exhibit a statistically significant difference. Nonetheless, both FRST and TRST demonstrated positive effects on 1RM squat and CMJ performance compared to baseline values for each method. Therefore, flywheel resistance training can be considered an effective alternative to traditional resistance training for enhancing strength levels in female basketball players.