bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is Capillary Electrophoresis a New Tool to Monitor Acute Lithium Poisoning in Human?†
Authors: Jamal Tsandni , Hennequin Carole , Gahoual Rabah , Leyris Annie , Beaudeux Jean-Louis , Baud Frédéric J , Houzé Pascal 
Published: February 2019

Abstract
A 38-year-old man was admitted in the intensive care unit (ICU) after supposed ingestion of 504 sustained-release tablets of Theralithe™ corresponding ~200 g of lithium carbonate. At the admission, ~19.5 h after ingestion, the patient was conscious with trembling limbs, intense thirst, profuse sweats and vomiting and lithium serum concentration was 14.2 mmol/L. Toxicological screenings performed in urine and serum, were negative. Patient was treated with continuous extrarenal epuration by continue veno-venous hemodiafiltration starting (CCVHDF) 24 h post-admission and was carried on until 64 h. After 11 days in ICU, the patient was dismissed to the service without sequelae, and transferred to a psychiatric unit. To follow lithium concentrations in serum, urines and dialysates, we developed a simple, rapid and reliable method by capillary zone electrophoresis (CZE). Separation was achieved in 7 min. The method was linear between 0.14 and 1.44 mmol/L for serum samples, and between 0.07 and to 1.44 mmol/L for urines and dialysates. Limits of quantification were 0.15 mmol/L and 0.07 mmol/L for serum and others fluids, respectively. Intra- and inter-day precisions expressed as CV were systematically inferior to 12.1% for serum and 8.2% for other fluids. Results obtained regarding precision, accuracy, recovery and stability were satisfying, with recoveries ranging from 91.0 to 102.0%. Serum, urine and dialysate samples were measured using CZE and flame photometry. We observed a strong correlation between both methods as assessed by linear regression and Bland–Altman analysis. For the intoxicated patient, the assay was successfully applied to serum, urine and dialysates to determine the amount of lithium present in circulation and excreted. Lithium amounts in dialysates were estimated to correspond to 89% of total lithium excreted during CCVHF session while urine excretion account only for 11%.
Did Bioethics Matter? A History of Autonomy, Consent, and Intersex Genital Surgery
Authors: Reis Elizabeth 
Published: February 2019

Abstract
This article argues that the rise of bioethics in the post-WWII era and the emergence of the legal doctrine of informed consent in the late 1950s should have had a greater impact on patients with intersex traits (atypical sex development) than they did, given their emphasis on respect for autonomy and beneficence toward patients. Instead, these progressive trends collided with a turn in intersex management toward infants, who were unable to provide autonomous consent about their medical care. Patient autonomy took a back seat as parents heeded physicians’ advice in an environment even more hierarchical than we know today. Intersex care of both infants and adults continues to need improvement. It remains an open question whether the abstract ideals of bioethics—respect, patient autonomy, and the requirement of informed consent—are alone adequate to secure that improvement, or whether legal actions (or the threat of litigation) or some other reforms will be required to effect such change.
A systematic study of soft X-ray pulse profiles of magnetars in quiescence
Authors: Hu Chin-Ping , Ng C-Y , Ho Wynn C G 
Published: February 2019

Abstract
Magnetars are neutron stars with extremely high surface magnetic fields. They show diverse X-ray pulse profiles in the quiescent state. We perform a systematic Fourier analysis of their soft X-ray pulse profiles. We find that most magnetars have a single-peaked profile and hence have low amplitudes of the second Fourier harmonics (A2). On the other hand, the pulsed fraction (PF) spreads over a wide range. We compared the results with theoretical profiles assuming various surface hotspot asymmetries, viewing geometries, and beaming functions. We found that a single value of the intensity ratio r between two antipodal hotspots is unable to reproduce the observed distribution of A2 and PF for all magnetars. The inferred r is probably anticorrelated with the thermal luminosity, implying that high-luminosity magnetars tend to have two symmetric hotspots. Our results are consistent with theoretical predictions, for which the existence of an evolving toroidal magnetic field breaks the symmetry of the surface temperature.
Emerging Technologies in Optimizing Pre-Intervention Workflow for Acute Stroke
Authors: Yaeger Kurt A , Shoirah Hazem , Kellner Christopher P , Fifi Johanna , Mocco J 
Published: February 2019

Abstract
Over the last several years, thrombectomy for large vessel occlusions (LVOs) has emerged as a standard of care for acute stroke patients. Furthermore, the time to reperfusion has been identified as a predictor of overall patient outcomes, and much effort has been made to identify potential areas to target in enhancing preintervention workflow. As medical technology and stroke devices improve, nearly all time points can be affected, from field stroke triage to automated imaging interpretation to mass mobile stroke code communications. In this article, we review the preintervention stroke workflow with specific regard to emerging technologies in improving time to reperfusion and overall patient outcomes.
History of Depression and Increased Risk of Sternal Wound Infection After Cardiothoracic Surgery: A Novel and Potentially Modifiable Risk Factor
Authors: Theodore Deborah A , Goodwin Renee D , Zhang Yuan (Vivian) , Schneider Nancy , Gordon Rachel J 
Published: February 2019

Abstract

Background
Sternal wound infection (SWI) is a leading cause of postoperative disease and death; the risk factors for SWI remain incompletely understood. The goal of the current study was to investigate the relationship between a preoperative history of depression and the risk of SWI after cardiothoracic surgery.

Methods
Among patients undergoing cardiothoracic surgery in a major academic medical center between 2007 and 2012, those in whom SWI developed (n = 129) were matched, by date of surgery, with those in whom it did not (n = 258). Multivariable logistic regression was used to examine the strength of relationships between risk factors and development of infection. History of depression was defined as a composite variable to increase the sensitivity of detection.

Results
History of depression as defined by our composite variable was associated with increased risk of SWI (adjusted odds ratio, 2.4; 95% confidence interval, 1.2–4.7; P = .01). Staphylococcus aureus was the most common organism isolated.

Conclusions
History of depression was associated with increased risk of SWI. Future prospective studies are warranted to further investigate this relationship. Depression is highly treatable, and increased efforts to identify and treat depression preoperatively may be a critical step toward preventing infection-related disease and death.
In Vitro Activity of Eravacycline in Combination with Colistin Against Carbapenem-Resistant A. baumannii Isolates
Authors: Selcuk Ozger H. , Cuhadar Tugba , Yildiz Serap Suzuk , Gulmez Zehra Demirbas , Dizbay Murat , Tunccan Ozlem Guzel , Kalkanci Ayşe , Simsek Husniye , Unaldi Ozlem 
Published: February 2019

AbstractThe synergistic activity of eravacycline in combination with colistin on carbapenem-resistant A.baumannii (CRAB) isolates was evaluated in this study. Minimum inhibitory concentrations (MICs) of eravacycline and colistin were determined by the broth microdilution method. MICs values ranged between 1 to 4 mg and 0,5 to 128 mg/L for eravacycline and colistin, respectively. In-vitro synergy between eravacycline and colistin was evaluated by using the chequerboard methodology. Synergistic activity was found in 10 % of the strains, and additive effect in 20 %. No antagonism was detected. Similar activity was also observed in colistin resistant CRAB isolates. The result of this study indicates that eravacycline and colistin combination may be a potential therapeutic option for the treatment of CRAB related infections.
A global synthesis reveals biodiversity-mediated benefits for crop production
Authors: Dainese Matteo , Martin Emily A. , Aizen Marcelo A. , Albrecht Matthias , Bartomeus Ignasi , Bommarco Riccardo , Carvalheiro Luisa G. , Chaplin-Kramer Rebecca , Gagic Vesna , Garibaldi Lucas A. , Ghazoul Jaboury , Grab Heather , Jonsson Mattias , Karp Daniel S. , Kennedy Christina M. , Kleijn David , Kremen Claire , Landis Douglas A. , Letourneau Deborah K. , Marini Lorenzo , Poveda Katja , Rader Romina , Smith Henrik G. , Tscharntke Teja , Andersson Georg K.S. , Badenhausser Isabelle , Baensch Svenja , Bezerra Antonio Diego M. , Bianchi Felix J.J.A. , Boreux Virginie , Bretagnolle Vincent , Caballero-Lopez Berta , Cavigliasso Pablo , Ćetković Aleksandar , Chacoff Natacha P. , Classen Alice , Cusser Sarah , da Silva e Silva Felipe D. , de Groot G. Arjen , Dudenhöffer Jan H. , Ekroos Johan , Fijen Thijs , Franck Pierre , Freitas Breno M. , Garratt Michael P.D. , Gratton Claudio , Hipólito Juliana , Holzschuh Andrea , Hunt Lauren , Iverson Aaron L. , Jha Shalene , Keasar Tamar , Kim Tania N. , Kishinevsky Miriam , Klatt Björn K. , Klein Alexandra-Maria , Krewenka Kristin M. , Krishnan Smitha , Larsen Ashley E. , Lavigne Claire , Liere Heidi , Maas Bea , Mallinger Rachel E. , Pachon Eliana Martinez , Martínez-Salinas Alejandra , Meehan Timothy D. , Mitchell Matthew G.E. , Molina Gonzalo A.R. , Nesper Maike , Nilsson Lovisa , O’Rourke Megan E. , Peters Marcell K. , Plećaš Milan , Potts Simon G. , Ramos Davi de L. , Rosenheim Jay A. , Rundlöf Maj , Rusch Adrien , Sáez Agustín , Scheper Jeroen , Schleuning Matthias , Schmack Julia , Sciligo Amber R. , Seymour Colleen , Stanley Dara A. , Stewart Rebecca , Stout Jane C. , Sutter Louis , Takada Mayura B. , Taki Hisatomo , Tamburini Giovanni , Tschumi Matthias , Viana Blandina F. , Westphal Catrin , Willcox Bryony K. , Wratten Stephen D. , Yoshioka Akira , Zaragoza-Trello Carlos , Zhang Wei , Zou Yi , Steffan-Dewenter Ingolf 
Published: February 2019

ABSTRACTHuman land use threatens global biodiversity and compromises multiple ecosystem functions critical to food production. Whether crop yield-related ecosystem services can be maintained by few abundant species or rely on high richness remains unclear. Using a global database from 89 crop systems, we partition the relative importance of abundance and species richness for pollination, biological pest control and final yields in the context of on-going land-use change. Pollinator and enemy richness directly supported ecosystem services independent of abundance. Up to 50% of the negative effects of landscape simplification on ecosystem services was due to richness losses of service-providing organisms, with negative consequences for crop yields. Maintaining the biodiversity of ecosystem service providers is therefore vital to sustain the flow of key agroecosystem benefits to society.
Localization microscopy at doubled precision with patterned illumination
Authors: Cnossen Jelmer , Hinsdale Taylor , Thorsen Rasmus Ø. , Schueder Florian , Jungmann Ralf , Smith Carlas S. , Rieger Bernd , Stallinga Sjoerd 
Published: February 2019

AbstractMINFLUX offers a breakthrough in single molecule localization precision, but suffers from a tiny field-of-view and a lack of practical parallelism. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron sized field-of-view. We show a near twofold improvement in precision over standard localization with the same photon count on DNA-origami nano-structures.
Classification of visual cortex plasticity phenotypes following treatment for amblyopia
Authors: Balsor Justin L. , Jones David G. , Murphy Kathryn M. 
Published: February 2019

AbstractMonocular deprivation (MD) during the critical period (CP) has enduring effects on visual acuity and the functioning of the visual cortex (V1). This experience-dependent plasticity has become a model for studying the mechanisms, especially glutamatergic and GABAergic receptors, that regulate amblyopia. Less is known, however, about treatment-induced changes to those receptors and if those changes differentiate treatments that support the recovery of acuity versus persistent acuity deficits. Here we use an animal model to explore the effects of 3 visual treatments started during the CP (n=24, 10 male and 14 female); binocular vision (BV) that promotes good acuity versus reverse occlusion (RO) and binocular deprivation (BD) that causes persistent acuity deficits. We measured recovery of a collection of glutamatergic and GABAergic receptor subunits in V1 and modeled recovery of kinetics for NMDAR and GABAAR. There was a complex pattern of protein changes that prompted us to develop an unbiased data-driven approach for these high-dimensional data analyses to identify plasticity features and construct plasticity phenotypes. Cluster analysis of the plasticity phenotypes suggests that BV supports adaptive plasticity while RO and BD promote a maladaptive pattern. The RO plasticity phenotype appeared more similar to adults with high expression of GluA2 and the BD phenotypes were dominated by GABAAα1, highlighting that multiple plasticity phenotypes can underlie persistent poor acuity. After 2-4 days of BV the plasticity phenotypes resembled normals, but only one feature, the GluN2A:GluA2 balance, returned to normal levels. Perhaps, balancing Hebbian (GluN2A) and homeostatic (GluA2) mechanisms is necessary for the recovery of vision.
MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure
Authors: Wu Zhihao , Tantray Ishaq , Lim Junghyun , Chen Songjie , Li Yu , Davis Zoe , Sitron Cole , Dong Jason , Gispert Suzana , Auburger Georg , Brandman Onn , Bi Xiaolin , Snyder Michael , Lu Bingwei 
Published: February 2019

SUMMARYMitochondrial dysfunction and proteostasis failure frequently coexist as hallmarks of neurodegenerative disease. How these pathologies are related is not well understood. Here we describe a phenomenon termed MISTERMINATE (mitochondrial stress-induced translational termination impairment and protein carboxyl terminal extension), which mechanistically links mitochondrial dysfunction with proteostasis failure. We show that mitochondrial dysfunction impairs translational termination of nuclear-encoded mitochondrial mRNAs including complex-I 30kD subunit (C-I30) mRNA, occurring on mitochondrial surface in Drosophila and mammalian cells. Ribosomes stalled at the normal stop codon continue to add to the C-terminus of C-I30 certain amino acids non-coded by mRNA template. C-terminally-extended C-I30 is toxic when assembled into C-I and forms aggregates in the cytosol. Enhancing co-translational quality control prevents C-I30 C-terminal extension and rescues mitochondrial and neuromuscular degeneration in a Parkinson’s disease model. These findings emphasize the importance of efficient translation termination and reveal unexpected link between mitochondrial health and proteome homeostasis mediated by MISTERMINATE.
Complete genome sequence of Bacillus velezensis JT3-1, a microbial germicide isolated from yak feces
Authors: Li Youquan , Li Xuan , Jia Dan , Liu Junlong , Wang Jinming , Liu Aihong , Liu Zhijie , Guan Guiquan , Liu Guangyuan , Luo Jianxun , Yin Hong 
Published: February 2019

AbstractBacillus velezensis JT3-1 is a probiotic strain isolated from feces of the domestic yak (Bos grunniens) in the Gansu province of China. It has strong antagonistic activity against Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, Mannheimia haemolytica, Staphylococcus hominis, Clostridium perfringens, and Mycoplasma bovis. These properties have made the JT3-1 strain the focus of commercial interest. In this study, we describe the complete genome sequence of JT3-1, with a genome size of 3,929,799 bp, 3761 encoded genes and an average GC content of 46.50%. Whole genome sequencing of Bacillus velezensis JT3-1 will lay a good foundation for elucidation of the mechanisms of its antimicrobial activity, and for its future application.
Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex
Authors: Mich John K. , Graybuck Lucas T. , Hess Erik E. , Mahoney Joseph T. , Kojima Yoshiko , Ding Yi , Somasundaram Saroja , Miller Jeremy A. , Weed Natalie , Omstead Victoria , Bishaw Yemeserach , Shapovalova Nadiya V. , Martinez Refugio A. , Fong Olivia , Yao Shenqin , Mortrud Marty , Chong Peter , Loftus Luke , Bertagnolli Darren , Goldy Jeff , Casper Tamara , Dee Nick , Opitz-Araya Ximena , Cetin Ali , Smith Kimberly A. , Gwinn Ryder P. , Cobbs Charles , Ko Andrew. L. , Ojemann Jeffrey G. , Keene C. Dirk , Silbergeld Daniel. L. , Sunkin Susan M. , Gradinaru Viviana , Horwitz Gregory D. , Zeng Hongkui , Tasic Bosiljka , Lein Ed S. , Ting Jonathan T. , Levi Boaz P. 
Published: February 2019

SummaryViral genetic tools to target specific brain cell types in humans and non-genetic model organisms will transform basic neuroscience and targeted gene therapy. Here we used comparative epigenetics to identify thousands of human neuronal subclass-specific putative enhancers to regulate viral tools, and 34% of these were conserved in mouse. We established an AAV platform to evaluate cellular specificity of functional enhancers by multiplexed fluorescent in situ hybridization (FISH) and single cell RNA sequencing. Initial testing in mouse neocortex yields a functional enhancer discovery success rate of over 30%. We identify enhancers with specificity for excitatory and inhibitory classes and subclasses including PVALB, LAMP5, and VIP/LAMP5 cells, some of which maintain specificityin vivoorex vivoin monkey and human neocortex. Finally, functional enhancers can be proximal or distal to cellular marker genes, conserved or divergent across species, and could yield brain-wide specificity greater than the most selective marker genes.
Self-advocacy and self-determination of autistic students: a review of the literature
Authors: Zuber William James , Webber Colin 
Published: February 2019

PurposeThe purpose of this paper is to examine current research on self-advocacy and self-determination of autistic students in order to provide an overview of the research and to critically evaluate researcher’s methods of inclusivity of autistic people. Additionally, this paper will critically analyse the discourse of the current research to assess the extent of deficit, stigma and pathology discourse.Design/methodology/approachResearch will be selected from a list of criteria which is to seek research that is inclusive of autistic people. The research will be analysed using elements of critical discourse analysis, critical disability studies and critical autism studies. The critical autism studies approach used in this paper is emancipatory to promote autistic scholarship, autistic inclusivity and autistic led research methods.FindingsThe result of this paper is that by prioritising, and including autistic individuals in the studies about them provides valuable educational insights and often challenges assumptions, stigmas and stereotypes of autistic individuals.Research limitations/implicationsThe findings of the paper may be limited by the selection of literature reviewed and generalizability, therefore, researchers are encouraged to explore further.Practical implicationsThis paper holds potential implications that question the consistency of current discourse and research into self-advocacy for autistic individuals in addition to providing effective research, teaching and support strategies based on insight. This paper also highlights some research that challenges assumptions of autistic individuals.Social implicationsThis paper challenges assumptions and stigmas associated with autistic individuals and demonstrates the importance of self-advocacy and self-determination. This research transforms the paradigm of autism and education practice that has the potential to improve autistic individuals’ education and ultimately, improve their lives.Originality/valueThis research is important and valuable as there is limited research in this area. The potential of this research is that it can shift the broad perceptions of autism and make improvements in education and autistic individuals lives.
Prosocial process fit: normatively expected purchasing increases the prosocial premium
Authors: Mathmann Frank , Pohlmeyer Lisa , Higgins E. Tory , Weeks Clinton 
Published: February 2019

PurposeThis paper aims to investigates the effect of normative expectations in the purchase process on consumers’ value perceptions for prosocial products (e.g. environmentally friendly products) relative to conventional non-prosocial products. It extends the literature on both prosocial products and regulatory fit.Design/methodology/approachFive factorial experiments are employed, testing diverse samples, including Dutch university students and American online panel participants from the general population.FindingsFindings show that regulatory fit between the prosocial product orientation and an emphasis on normative expectations in the purchase process (termed prosocial process fit) increases perceptions of prosocial product value (relative to conventional products). This effect is mediated by engagement.Research limitations/implicationsThe current research is limited to investigating how value perceptions of prosocial products can be increased (i.e. through prosocial process fit). Future research is warranted that analogously considers conditions that would increase value for non-prosocial products as well (e.g. by creating a fit with a non-prosocial process).Practical implicationsThe research shows how prosocial manufacturers and retailers can redesign the purchase process to increase customers’ engagement, perceptions of prosocial product value and prosocial product purchase.Social implicationsThis work serves to explain differences in consumers’ value perceptions for prosocial products. Hence, it shows how socially responsible consumption can be better supported in society.Originality/valueThis work demonstrates a new kind of regulatory fit based on fit between prosocial products and normative expectations in the purchase process (i.e. moving beyond the types of regulatory fit previously examined in this context, such as with fit between regulatory focus orientation and goal pursuit). The authors use this to provide a much needed explanation for the heterogeneity in the literature regarding the value that consumers experience for prosocial products relative to conventional ones.