bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors related to the outcome of prophylactic platelet transfusions in patients with hematologic malignancies: an observational study
Authors: Seigeot Audrey , Desmarets Maxime , Rumpler Anne , Leroux Franck , Deconinck Eric , Monnet Elisabeth , Bardiaux Laurent 
Published: March 2018

BACKGROUNDA better knowledge of the connections between platelet concentrate (PC) characteristics and transfusion outcomes in day‐to‐day practice would help improve the selection process of the most appropriate PC.STUDY DESIGN AND METHODSIn this study of prophylactic platelet transfusions in patients with hematologic malignancies between 2002 and 2012, outcome criteria were corrected count increments (CCIs) and platelet transfusion intervals (TIs, in days). Studied characteristics were ABO matching status, platelet source, dose, storage duration, irradiation, washing, and transfusion sequence number (TSN). The analysis consisted of multivariable linear mixed‐effects models with adjustments for patient diagnosis, sex, and type of treatment.RESULTSOverall, 869 patients and 6662 platelet transfusions were analyzed. For each day after the second day of storage, the CCI and TI decreased by 0.88 and 0.06 day, respectively. Compared to ABO‐identical, transfusion with major ABO‐incompatible PCs decreased the CCI and TI by 0.79 and 0.21 day, respectively. Platelet washing reduced the CCI and TI by 2.28 and 0.24 day, respectively. There was no significant association between platelet source or irradiation and CCI or TI. TI increased as the platelet dose per kg increased. Both CCI and TI decreased as the TSN increased.CONCLUSIONTransfusion outcomes were significantly related to several PC‐related factors. Associations for ABO matching status and storage duration were stronger than previously reported. Taking into account such factors when selecting a PC for transfusion could be beneficial to the recipient.
Radiographic and computed tomographic appearance of caseous lymphadenitis in a goat
Authors: Vilaplana Grosso Federico , Tinkler Stacy , Sola Mario , Miller Margaret , Heng Hock Gan 
Published: March 2018

AbstractA 2‐year‐old Boer doe was presented with respiratory distress and severe inspiratory dyspnea. Cervical and thoracic radiographs revealed a marked retropharyngeal soft tissue mass effect, several mineralized pharyngeal and retropharyngeal structures, and a pulmonary mass. Computed tomography (CT) revealed a severely enlarged right medial retropharyngeal lymph node, which caused laryngeal compression, leading to upper airway obstruction. Multiple cervical and thoracic lymph nodes, and the pulmonary mass had an irregular layered/laminated pattern of mineralization. Imaging findings were consistent with caseous lymphadenitis, and further confirmed with culture, necropsy, and histopathology. This is the first report of CT appearance of caseous lymphadenitis in a goat.
Long‐term precipitation trend analysis in Europe and in the Mediterranean basin
Authors: Caloiero Tommaso , Caloiero Paola , Frustaci Francesco 
Published: March 2018

AbstractThe spatiotemporal analysis of a region's precipitation climate regime could be particularly interesting for many fields of applied sciences, such as climatology, hydrology and water resources management. In this study, the precipitation trends at seasonal and annual scale, over a large area of the northern Hemisphere have been analysed based on a globally gridded precipitation data sets of monthly observations. In particular, among the several data sets available in literature, with spatial resolutions of 0.5° longitude/latitude, in this work the Global Precipitation Climatology Centre Full Data Reanalysis Version 6 data set has been used. The broad extension of the area under investigation allowed a better understanding of trend precipitation patterns over space. In fact, yearly results evidenced a marked negative rainfall tendency in the eastern Mediterranean (more than −20 mm/10 years) and in North Africa (until −16 mm/10 years), while a relatively large positive trend (more than 20 mm/10 years) in central and northern Europe has been observed.
Multiple genetically modified GTKO/hCD46/HLA‐E/hβ2−mg porcine hearts are protected from complement activation and natural killer cell infiltration during ex vivo perfusion with human blood
Authors: Abicht Jan‐Michael , Sfriso Riccardo , Reichart Bruno , Längin Matthias , Gahle Katja , Puga Yung Gisella L. , Seebach Jörg D. , Rieben Robert , Ayares David , Wolf Eckhard , Klymiuk Nikolai , Baehr Andrea , Kind Alexander , Mayr Tanja , Bauer Andreas 
Published: March 2018

AbstractBackgroundIn pig‐to‐human xenotransplantation, early cellular rejection reactions are mediated by natural killer cells (NK cells). Human NK cells are inhibited by HLA‐E via CD94/NKG2A receptors. To protect porcine grafts against human NK cell responses, transgenic GTKO pigs expressing hCD46 and HLA‐E have been generated. The aim of this study was to test the effect of this genetic modification on xenogeneic, and in particular human NK cell response, using an ex vivo perfusion model of pig hearts with human blood.MethodsCardiopleged and explanted genetically modified (gm) pig hearts (GTKO/hCD46/HLA‐E/hβ2‐microglobulin) and wild‐type (wt) controls (n = 6 each) were reperfused and tested in an 8 hours ex vivo perfusion system using freshly drawn human blood. Cardiac function was evaluated during a 165‐minute period in working heart mode. Myocardial damage, antibody deposition, complement activation, and coagulation parameters were evaluated histologically at the end of perfusion. The number of NK cells in the perfusate was determined by flow cytometry at baseline and at 8 hours; tissue infiltration by NK cells was quantified by immunofluorescence microscopy using NKp46 staining of frozen sections.ResultsDeposition of IgG (1.2 ± 1 × 107 vs 8.8 ± 2.9 × 106; P < .01), IgM (4.4 ± 3.7 × 106 vs 1.7 ± 1.2 × 106; P < .01), and the complement activation product C4b/c (3.5 ± 1.3 × 106 vs 2.3 × 106 ± 9.4 × 105; P > .01) was lower in gm than wt hearts. NK cell percentages of leukocytes in the perfusate decreased from 0.94 ± 0.77% to 0.21 ± 0.25% (P = .04) during xenoperfusion of wt hearts. In contrast, the ratio of NK cells did not decrease significantly in the gm hearts. In this group, NK cell myocardial infiltration after 480 minutes of perfusion was lower than in wt organs (2.5 ± 3.7 × 104/mm3 vs 1.3 ± 1.4 × 105/mm3; P = .0001). The function of gm hearts was better preserved compared to wt organs, as demonstrated by higher cardiac index during the first 2 hours of ex vivo perfusion.ConclusionGTKO, hCD46, and HLA‐E expression in porcine hearts reduced complement deposition, complement dependent injury, and myocardial NK cell infiltration during perfusion with human blood. This tested combination of genetic modifications may minimize damage from acute human‐anti‐pig rejection reactions and improve myocardial function after xenotransplantation.
Improvement of Prediction of Noncontact Injury in Elite Australian Footballers With Repeated Exposure to Established High-Risk Workload Scenarios
Authors: Colby Marcus J. , Dawson Brian , Peeling Peter , Heasman Jarryd , Rogalski Brent , Drew Michael K. , Stares Jordan 
Published: March 2018

Objectives: To assess the effect of multiple high-risk-scenario (HRS) exposures on noncontact injury prediction in elite Australian footballers. Design: Retrospective cohort study. Methods: Sessional workload data (session rating of perceived exertion, global positioning system–derived distance, sprint distance, and maximum velocity) from 1 club (N = 60 players) over 3 seasons were collated; several established HRSs were also defined. Accumulated HRS sessional exposures were calculated retrospectively (previous 1–8 wk). Noncontact injury data were documented. Univariate and multivariate Poisson regression models determined injury incidence rate ratios (IRRs) while accounting for moderating effects (preseason workload volume and playing experience). Model performance was evaluated using receiver operating characteristics (area under curve). Results: Very low (0–8 sessions: IRR = 5.76; 95% confidence interval [CI], 1.69–19.66) and very high (>15 sessions: IRR = 4.70; 95% CI, 1.49–14.87) exposures to >85% of an individual’s maximal velocity over the previous 8 wk were associated with greater injury risk compared with moderate exposures (11–12 sessions) and displayed the best model performance (area under curve = 0.64). A single session corresponding to a very low chronic load condition over the previous week for all workload variables was associated with increased injury risk, with sprint distance (IRR = 3.25; 95% CI, 1.95–5.40) providing the most accurate prediction model (area under curve = 0.63). Conclusions: Minimal exposure to high-velocity efforts (maximum speed exposure and sprint volume) was associated with the greatest injury risk. Being underloaded may be a mediator for noncontact injury in elite Australian football. Preseason workload and playing experience were not moderators of this effect.
The Influence of Match-Day Napping in Elite Female Netball Athletes
Authors: O’Donnell Shannon , Beaven Christopher M. , Driller Matthew 
Published: March 2018

Purpose: To assess the effect of match-day napping and duration of naps on perceptual and performance indices in elite female netball players over 2 consecutive netball seasons. Methods: A total of 14 elite female netball athletes (mean [SD]; age = 23 [6] y) participated in an observational study over 26 competition matches. On each match day, athletes provided information on their napping habits and perceived energy levels, then performed 3 countermovement jumps 3 h:30 min prior to the start of the match. One hour after the match, subjective player performance ratings from the players and 2 members of the coaching staff were obtained. Naps were characterized into 3 conditions for analysis: no nap (NN), <20-min nap (SHORT), and ≥20-min nap (LONG). Results: A significant difference in peak jump velocity was observed between the SHORT and the NN condition in favor of the shorter nap (3.23 [0.26] and 3.07 [0.36] m·s−1, respectively, d = 0.34, P < .05). A moderate, significant difference (d = 0.85; P < .05) was observed for the coach rating of performance (out of 10) between the SHORT and the NN condition (7.2 [0.8] and 6.4 [0.9], respectively) in favor of SHORT. Conclusions: The findings from the study would suggest that a short nap (<20 min) on the day of competition can enhance jump velocity and improve subjective performance in elite netball players, as assessed by coaching staff.
Upper Trunk–Pelvis Coordination During Running Using the Continuous Relative Fourier Phase Method
Authors: Li Yumeng , Kakar Rumit S. , Walker Marika A. , Guan Li , Simpson Kathy J. 
Published: March 2018

The upper trunk–pelvic coordination patterns used in running are not well understood. The purposes of this study are to (1) test the running speed effect on the upper trunk–pelvis axial rotation coordination and (2) present a step-by-step guide of the relative Fourier phase algorithm, as well as some further issues to consider. A total of 20 healthy young adults were tested under 3 treadmill running speeds using a 3-dimensional motion capture system. The upper trunk and pelvic segmental angles in axial rotation were calculated, and the coordination was quantified using the relative Fourier phase method. Results of multilevel modeling indicated that running speed did not significantly contribute to the changes in coordination in a linear pattern. A qualitative template analysis suggested that participants displayed different change patterns of coordination as running speed increased. Participants did not significantly change the upper trunk and pelvis coordination mode in a linear pattern at higher running speeds, possibly because they employed different motion strategies to achieve higher running speeds and thus displayed large interparticipant variations. For most of our runners, running at a speed deviated from the preferred speed could alter the upper trunk–pelvis coordination. Future studies are still needed to better understand the influence of altered coordination on running performance and injuries.
Individuals With Knee Osteoarthritis Demonstrate Interlimb Asymmetry in Pedaling Power During Stationary Cycling
Authors: Buddhadev Harsh H. , Crisafulli Daniel L. , Suprak David N. , San Juan Jun G. 
Published: March 2018

Cycling is commonly prescribed for physical rehabilitation of individuals with knee osteoarthritis (OA). Despite the known therapeutic benefits, no research has examined interlimb symmetry of power output during cycling in these individuals. We investigated the effects of external workload and cadence on interlimb symmetry of crank power output in individuals with knee OA versus healthy controls. A total of 12 older participants with knee OA and 12 healthy sex- and age-matched controls were recruited. Participants performed 2-minute bouts of stationary cycling at 4 workload-cadence conditions (75 W at 60 rpm, 75 W at 90 rpm, 100 W at 60 rpm, and 100 W at 90 rpm). Power output contribution of each limb toward total crank power output was computed over 60 crank cycles from the effective component of pedal force, which was perpendicular to the crank arm. Across the workload-cadence conditions, the knee OA group generated significantly higher power output with the severely affected leg compared with the less affected leg (10% difference;P = .02). Healthy controls did not show interlimb asymmetry in power output (0.1% difference;P> .99). For both groups, interlimb asymmetry was unaffected by external workload and cadence. Our results indicate that individuals with knee OA demonstrate interlimb asymmetry in crank power output during stationary cycling.
TV Viewing in 60,202 Adults From the National Brazilian Health Survey: Prevalence, Correlates, and Associations With Chronic Diseases
Authors: Werneck André O. , Cyrino Edilson S. , Collings Paul J. , Ronque Enio R.V. , Szwarcwald Célia L. , Sardinha Luís B. , Silva Danilo R. 
Published: March 2018

Background: This study describes the levels and patterns of television (TV) viewing in Brazilian adults and investigates associations of TV viewing with hypertension, type 2 diabetes, and heart disease. Methods: Data from the Brazilian Health Survey, a nationally representative survey that was conducted in 2013 (N = 60,202 men and women aged ≥18 y), were used. Information regarding TV viewing, physician diagnoses of type 2 diabetes, hypertension, and heart disease was collected via interview-administered questionnaire. Data on covariables (including chronological age, educational status, skin color, sodium consumption, sugar consumption, tobacco smoking, alcohol consumption, and leisure-time physical activity) were also self-reported. Logistic regression models and population attributable fractions were used for the etiological analyses. Results: The prevalence (95% confidence interval) of >4 hours per day of TV viewing was 12.7% (12.0–13.4) in men and 17.5% (16.8–18.3) in women. Men and women being younger or older, moderately educated, living alone, smoking tobacco, and drinking alcohol were associated with higher reported TV viewing time. Odds ratios (95% confidence interval) revealed that >4 hours per day of TV viewing was associated with type 2 diabetes [male: 1.64 (1.23–2.17) and female: 1.33 (1.09–1.63)], hypertension [male: 1.36 (1.14–1.63) and female: 1.20 (1.05–1.37)], and heart disease [male: 1.96 (1.43–2.69) and female: 1.30 (1.00–1.68)]. Exceeding 4 hours per day of TV viewing was responsible for 6.8% of type 2 diabetes, 3.7% of hypertension, and 7.5% of heart disease cases. Conclusions: Independent of covariates, >4 hours per day of TV viewing was associated with type 2 diabetes, hypertension, and heart disease. High volumes of TV viewing are prevalent and appear to contribute to chronic disease burden.
“You Can’t Just Start and Expect It to Work”: An Investigation of Strategic Capacity Building in Community Sport Organizations
Authors: Millar Patti , Doherty Alison 
Published: March 2018

Capacity building is a targeted approach to addressing organizational challenges by focusing development efforts on specific needs. Utilizing Millar and Doherty’s process model of capacity building, the purpose of this study was to (a) gain insight into the nature of the conditions and processes of capacity building in the community sport context and (b) examine the veracity of the proposed model. Interviews were conducted with organizational members from two community sport organizations that were purposefully chosen and happened to have introduced new programs: one that experienced successful capacity building that enhanced program and service delivery and one that experienced unsuccessful capacity building where organizational needs were not effectively addressed. Findings revealed that the thoroughness of the needs assessment, the selection of appropriate capacity building strategies, and readiness to build capacity were key factors in the (lack of) success of the capacity building efforts. Implications for practice and future research on organizational capacity building are presented.
Baculovirus AC102 Is a Nucleocapsid Protein That Is Crucial for Nuclear Actin Polymerization and Nucleocapsid Morphogenesis
Authors: Hepp Susan E. , Borgo Gina M. , Ticau Simina , Ohkawa Taro , Welch Matthew D. 
Published: March 2018

ABSTRACT
The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the type species of alphabaculoviruses, is an enveloped DNA virus that infects lepidopteran insects and is commonly known as a vector for protein expression and cell transduction. AcMNPV belongs to a diverse group of viral and bacterial pathogens that target the host cell actin cytoskeleton during infection. AcMNPV is unusual, however, in that it absolutely requires actin translocation into the nucleus early in infection and actin polymerization within the nucleus late in infection coincident with viral replication. Of the six viral factors that are sufficient, when coexpressed, to induce the nuclear localization of actin, only AC102 is essential for viral replication and the nuclear accumulation of actin. We therefore sought to better understand the role of AC102 in actin mobilization in the nucleus early and late in infection. Although AC102 was proposed to function early in infection, we found that AC102 is predominantly expressed as a late protein. In addition, we observed that AC102 is required for F-actin assembly in the nucleus during late infection, as well as for proper formation of viral replication structures and nucleocapsid morphogenesis. Finally, we found that AC102 is a nucleocapsid protein and a newly recognized member of a complex consisting of the viral proteins EC27, C42, and the actin polymerization protein P78/83. Taken together, our findings suggest that AC102 is necessary for nucleocapsid morphogenesis and actin assembly during late infection through its role as a component of the P78/83-C42-EC27-AC102 protein complex.

IMPORTANCE
The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an important biotechnological tool for protein expression and cell transduction, and related nucleopolyhedroviruses are also used as environmentally benign insecticides. One impact of our work is to better understand the fundamental mechanisms through which AcMNPV exploits the cellular machinery of the host for replication, which may aid in the development of improved baculovirus-based research and industrial tools. Moreover, AcMNPV's ability to mobilize the host actin cytoskeleton within the cell's nucleus during infection makes it a powerful cell biological tool. It is becoming increasingly clear that actin plays important roles in the cell's nucleus, and yet the regulation and function of nuclear actin is poorly understood. Our work to better understand how AcMNPV relocalizes and polymerizes actin within the nucleus may reveal fundamental mechanisms that govern nuclear actin regulation and function, even in the absence of viral infection.
Can virtual autopsy with postmortem CT improve clinical diagnosis of cause of death? A retrospective observational cohort study in a Dutch tertiary referral centre
Authors: Sonnemans Lianne J P , Kubat Bela , Prokop Mathias , Klein Willemijn M 
Published: March 2018

ObjectiveTo investigate whether virtual autopsy with postmortem CT (PMCT) improves clinical diagnosis of the immediate cause of death.DesignRetrospective observational cohort study. Inclusion criteria: inhospital and out-of-hospital deaths over the age of 1 year in whom virtual autopsy with PMCT and conventional autopsy were performed. Exclusion criteria: forensic cases, postmortal organ donors and cases with incomplete scanning procedures. Cadavers were examined by virtual autopsy with PMCT prior to conventional autopsy. The clinically determined cause of death was recorded before virtual autopsy and was then adjusted with the findings of virtual autopsy. Using conventional autopsy as reference standard, we investigated the increase in sensitivity for immediate cause of death, type of pathology and anatomical system involved before and after virtual autopsy.SettingTertiary referral centre.Participants86 cadavers that underwent conventional and virtual autopsy between July 2012 and June 2016.InterventionPMCT consisted of brain, cervical spine and chest–abdomen–pelvis imaging. Conventional autopsy consisted of thoracoabdominal examination with/without brain autopsy.Primary and secondary outcome measuresIncrease in sensitivity for the immediate cause of death, type of pathology (infection, haemorrhage, perfusion disorder, other or not assigned) and anatomical system (pulmonary, cardiovascular, gastrointestinal, other or not assigned) involved, before and after virtual autopsy.ResultsUsing PMCT, the sensitivity for immediate cause of death increased with 12% (95% CI 2% to 22%) from 53% (41% to 64%) to 64% (53% to 75%), with 18% (9% to 27%) from 65% (54% to 76%) to 83% (73% to 91%) for type of pathology and with 19% (9% to 30%) from 65% (54% to 76%) to 85% (75% to 92%) for anatomical system.ConclusionWhile unenhanced PMCT is an insufficient substitute for conventional autopsy, it can improve diagnosis of cause of death over clinical diagnosis alone and should therefore be considered whenever autopsy is not performed.