bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patient-Derived Cancer Organoid Cultures to Predict Sensitivity to Chemotherapy and Radiation
Authors: Pasch Cheri A. , Favreau Peter F. , Yueh Alexander E. , Babiarz Christopher P. , Gillette Amani A. , Sharick Joe T. , Karim Mohammad Rezaul , Nickel Kwangok P. , DeZeeuw Alyssa K. , Sprackling Carley M. , Emmerich Philip B. , DeStefanis Rebecca A. , Pitera Rosabella T. , Payne Susan N. , Korkos Demetra P. , Clipson Linda , Walsh Christine M. , Miller Devon , Carchman Evie H. , Burkard Mark E. , Lemmon Kayla K. , Matkowskyj Kristina A. , Newton Michael A. , Ong Irene M. , Bassetti Michael F. , Kimple Randall J. , Skala Melissa C. , Deming Dustin A. 
Published: June 2019

Abstract

Purpose:
Cancer treatment is limited by inaccurate predictors of patient-specific therapeutic response. Therefore, some patients are exposed to unnecessary side effects and delays in starting effective therapy. A clinical tool that predicts treatment sensitivity for individual patients is needed.

Experimental Design:
Patient-derived cancer organoids were derived across multiple histologies. The histologic characteristics, mutation profile, clonal structure, and response to chemotherapy and radiation were assessed using bright-field and optical metabolic imaging on spheroid and single-cell levels, respectively.

Results:
We demonstrate that patient-derived cancer organoids represent the cancers from which they were derived, including key histologic and molecular features. These cultures were generated from numerous cancers, various biopsy sample types, and in different clinical settings. Next-generation sequencing reveals the presence of subclonal populations within the organoid cultures. These cultures allow for the detection of clonal heterogeneity with a greater sensitivity than bulk tumor sequencing. Optical metabolic imaging of these organoids provides cell-level quantification of treatment response and tumor heterogeneity allowing for resolution of therapeutic differences between patient samples. Using this technology, we prospectively predict treatment response for a patient with metastatic colorectal cancer.

Conclusions:
These studies add to the literature demonstrating feasibility to grow clinical patient-derived organotypic cultures for treatment effectiveness testing. Together, these culture methods and response assessment techniques hold great promise to predict treatment sensitivity for patients with cancer undergoing chemotherapy and/or radiation.
A Novel Oxoglutarate Dehydrogenase-Like Mediated miR-214/TWIST1 Negative Feedback Loop Inhibits Pancreatic Cancer Growth and Metastasis
Authors: Liu Yao , Meng Fanzheng , Wang Jiabei , Liu Mingyang , Yang Guangchao , Song Ruipeng , Zheng Tongsen , Liang Yingjian , Zhang Shugeng , Yin Dalong , Wang Jizhou , Yang Haiyan , Pan Shangha , Sun Bo , Han Jihua , Sun Jing , Lan Yaliang , Wang Yan , Liu Xirui , Zhu Mingxi , Cui Yifeng , Zhang Bo , Wu Dehai , Liang Shuhang , Liu Yufeng , Song Xuan , Lu Zhaoyang , Yang Jingxuan , Li Min , Liu Lianxin 
Published: June 2019

Abstract

Purpose:
As a main rate-limiting subunit of the 2-oxoglutarate dehydrogenase multienzyme complex, oxoglutarate dehydrogenase like (OGDHL) is involved in the tricarboxylic acid cycle, and frequently downregulated in human carcinoma and suppresses tumor growth. However, little is known about the role of OGDHL in human cancer, especially pancreatic cancer. Our goal is to study the underlying mechanism and define a novel signaling pathway controlled by OGDHL modulating pancreatic cancer progression.

Experimental Design:
The expression and functional analysis of OGDHL, miR-214, and TWIST1 in human pancreatic cancer tissues, cell lines, and xenograft tumor model were investigated. The correlations between OGDHL and those markers were analyzed.

Results:
OGDHL was downregulated in human pancreatic cancer and predicted poor prognosis. OGDHL overexpression inhibited migration and invasion of pancreatic cancer cells and suppressed pancreatic cancer tumor growth. OGDHL was shown to be negatively regulated by miR-214. TWIST1 upregulation induced miR-214 expression in pancreatic cancer. OGDHL suppressed TWIST1 expression through promoting ubiquitin-mediated proteasomal degradation of HIF1α and regulating AKT pathways. A combination of OGDHL downregulation and TWIST1 and miR-214 overexpression predicted worse prognosis in patients with pancreatic cancer.

Conclusions:
We demonstrated the prognostic value of OGDHL, miR-214, and TWIST1 in pancreatic cancer, and elucidated a novel pathway in OGDHL-regulated inhibition of pancreatic cancer tumorigenesis and metastasis. These findings may lead to new targeted therapy for pancreatic cancer through regulating OGDHL, miR-214, and TWIST1.
Distinguishing Tumor from Associated Fibrosis to Increase Diagnostic Biopsy Yield with Polarization-Sensitive Optical Coherence Tomography
Authors: Hariri Lida P. , Adams David C. , Applegate Matthew B. , Miller Alyssa J. , Roop Benjamin W. , Villiger Martin , Bouma Brett E. , Suter Melissa J. 
Published: June 2019

Abstract

Purpose:
With recent advancements in personalized medicine, biopsies must contain sufficient tumor for histologic diagnosis and molecular testing. However, inadvertent biopsy of tumor-associated fibrosis compromises tumor yield, resulting in delayed diagnoses and/or repeat procedures when additional tumor is needed. The ability to differentiate tumor from fibrosis intraprocedurally during biopsy could significantly increase tumor yield. Polarization-sensitive optical coherence tomography (PS-OCT) is an imaging modality that is endoscope- and/or needle-compatible, and provides large volumetric views of tissue microstructure with high resolution (∼10 μm) while simultaneously measuring birefringence of organized tissues such as collagen. We aim to determine whether PS-OCT can accurately detect and distinguish tumor-associated fibrosis from tumor.

Experimental Design:
PS-OCT was obtained ex vivo in 64 lung nodule samples. PS-OCT birefringence was measured and correlated to collagen content in precisely matched histology, quantified on picrosirius red (PSR) staining.

Results:
There was a strong positive correlation between PS-OCT measurement of birefringent fibrosis and total collagen content by PSR (r = 0.793; P &lt; 0.001). In addition, PS-OCT was able to accurately classify tumor regions with &gt;20% fibrosis from those with low fibrosis (≤20%) that would likely yield higher tumor content (P &lt; 0.0001).

Conclusions:
PS-OCT enables accurate fibrosis detection and can distinguish tumor regions with low fibrosis. PS-OCT has significant potential for clinical impact, as the ability to differentiate tumor from fibrosis could be used to guide intraprocedural tissue sampling in vivo, or for rapid biopsy adequacy assessment ex vivo, to increase diagnostic tumor yield essential for patient care and research.
Epidemiology of Cardiac Amyloidosis–Associated Heart Failure Hospitalizations Among Fee-for-Service Medicare Beneficiaries in the United States
Authors: Gilstrap Lauren G. , Dominici Francesca , Wang Yun , El-Sady M. Samir , Singh Amitoj , Di Carli Marcelo F. , Falk Rodney H. , Dorbala Sharmila 
Published: June 2019

Background:
Cardiac amyloidosis is a substantially underdiagnosed disease, and contemporary estimates of the epidemiology of amyloidosis are lacking. This study aims to determine the incidence and prevalence of cardiac amyloidosis among Medicare beneficiaries from 2000 to 2012.

Methods and Results:

Medicare beneficiaries were counted in the prevalence cohort in each year they had (1) ≥1 principal or secondary
International Classification of Diseases
,
Ninth Revision
code for amyloidosis and (2) ≥1 principal or secondary
International Classification of Diseases, Ninth Revision
code for heart failure or cardiomyopathy within 2 years after the systemic amyloidosis code. A beneficiary was counted in the incidence cohort only during the first year in which they met criteria. Primary outcomes included the prevalence and incidence of hospitalizations for cardiac amyloidosis. There were 4746 incident cases of cardiac amyloidosis in 2012 and 15 737 prevalent cases in 2012. There was also a significant increase in the prevalence rate (8 to 17 per 100 000 person-years) and incidence rate (18 to 55 per 100 000 person-years) from 2000 to 2012, most notable after 2006. Incidence and prevalence increased substantially more among men, the elderly, and in blacks.

Conclusions:
The incidence and prevalence rates of cardiac amyloidosis are higher than previously thought. The incidence and prevalence rates of cardiac amyloidosis among hospitalized patients have increased since 2000, particularly among specific patient subgroups and after 2006, suggesting improved amyloidosis awareness and higher diagnostic rates with noninvasive imaging. In light of these trends, cardiac amyloidosis should be considered during the initial work up of patients ≥65 years old hospitalized with heart failure.
RNA Sequencing Reveals the Activation of Wnt Signaling in Low Flow Rate Brain Arteriovenous Malformations
Authors: Huo Ran , Fu Weilun , Li Hao , Jiao Yuming , Yan Zihan , Wang Linjian , Wang Jie , Wang Shuo , Cao Yong , Zhao Jizong 
Published: June 2019

Background

The blood flow rate of brain arteriovenous malformations (
bAVM
s) is an important clinical characteristic closely associated with the hemorrhage risk and radiosurgery obliteration rate of
bAVM
s. However, the underlying molecular properties remain unclear. To identify potential key molecules, signaling pathways, and vascular cell types involved, we compared gene expression profiles between
bAVM
s with high flow rates and low flow rates (
LFR
) and validated the functions of selected key molecules in vitro.

Methods and Results

We performed
RNA
‐sequencing analysis on 51 samples, including 14 high flow rate
bAVM
s and 37
LFR bAVM
s. Functional pathway analysis was performed to identify potential signals influencing the flow rate phenotype of
bAVM
s. Candidate genes were investigated in
bAVM
specimens by immunohistochemical staining. Migration, tube formation, and proliferation assays were used to test the effects of candidate genes on the phenotypic properties of cultured human umbilical vein endothelial cells and human brain vascular smooth muscle cells. We identified 250 upregulated and 118 downregulated genes in
LFR bAVM
s compared with high flow rate
bAVM
s. Wnt signaling was activated in the
LFR
group via upregulation of
FZD
10 and
MYOC
. Immunohistochemical staining showed that vascular endothelial and smooth muscle cells of
LFR bAVM
s exhibited increased
FZD
10 and
MYOC
expression. Experimentally elevating these genes promoted human umbilical vein endothelial cells and migration and tube formation by activating canonical Wnt signaling in vitro.

Conclusions

Our results suggest that canonical Wnt signaling mediated by
FZD
10 and
MYOC
is activated in vascular endothelial and smooth muscle cells in
LFR bAVM
s.
Jung’s Fantasies of Africa and Africa’s Healing of Analytical Psychology
Authors: Brooke Roger 
Published: June 2019

Abstract
Jung’s dreams about Africa reveal the Whiteness and colonialist assumptions typical of the twentieth century educated European. Jung’s visits to Africa and New Mexico, and his dreams are critically discussed, showing how, even decades later, Jung failed to use his own theory of dreaming with regard to his own dreams. The compensatory function of his dreams was never effected, and his transference fantasies of Africa and blackness were reinforced rather than analyzed. There were unfortunate consequences for the development of his thinking and his understanding of the individuation process, since his oppositional thinking in terms of White and Black remained as a concrete transference fantasy as well as a colonialist attitude towards his internal world. The Nguni term ubuntu, will be used to reimagine individuation in more explicitly ethical and socially embedded ways. With regard to the development of consciousness, a distinction is developed between the withdrawal of projections and as a helpful therapeutic issue and as an epistemological approach to the place of meaning. If Jung’s dreams of Africa had managed to “heal” him, Jungian psychology would look rather like it does today, because the way out of Jung’s Colonialism is to be found in Jung’s life and work, especially in his alchemical studies.
The Love-Hate Relationship between Jung and Modern Art
Authors: Dib Roula-Maria 
Published: June 2019

Abstract
This paper takes Jung’s essay ‘Ulysses’ as a case study in order to elucidate his opinion on the relationship between modern artists and mental insanity. The study seeks to contradict a common misconception that Jung ‘diagnoses’ modern artists such as Pablo Picasso and James Joyce with schizophrenia; instead, the paper sheds light on the cultural (not pathological) connections he makes between modern artists and art. The study examines Jung’s writings on modern art and aesthetics, aiming to dismantle some misinterpretations that might make his attitude toward them appear to be a diagnostic one. Above all, I suggest that through his reading of Ulysses, Jung shows how the feelings of confusion that arose in him can actually be reconstructive; this leads to what I would dub a ‘psychic synthesis’ through modern art. Finally, after clarifying Jung’s standpoint on modern art and insanity, this paper seeks to significantly contribute a revaluation of Jung on modernism.
The “Fused Horizon” of Abolitionism and Islam
Authors: Mathews Nathaniel 
Published: June 2019

Abstract
This article considers slavery and abolition in Muslim societies globally as a historical and historicist problem. I argue that the changes in popular consensus among Muslims about the desirability and permissibility of owning slaves is primarily due to a Gadamerian “fused horizon” of abolitionism and Islam. I theorize one site of its emergence from interreligious African cooperation in New World slave rebellions. By studying slavery as a global process and parochializing the boundaries between the civilizational and regional histories of Islam, Europe, Africa, Asia and the Americas, there emerges a radical critique of slavery and capitalism that combines elements of both abolitionism and Islam. The historical experience of enslaved people provides an experiential and evidential basis for this new hermeneutical horizon.
RETRACTED ARTICLE: LncRNA TUBA4B functions as a competitive endogenous RNA to inhibit gastric cancer progression by elevating PTEN via sponging miR-214 and miR-216a/b
Authors: Guo Jianbo , Li Yan , Duan He , Yuan Lu 
Published: June 2019

Abstract
Background
Emerging evidence demonstrates that long non-coding RNA (lncRNA) is an important regulator in tumorigenesis and development. Tubulin Alpha 4B (TUBA4B), a novel lncRNA, was recently proposed as a tumor suppressor in several human cancers. However, its role in gastric cancer (GC) remains unclear. In this study, we aimed to investigate the expression level, clinical implication, biological function and potential regulatory mechanism of TUBA4B in GC.

Methods
qRT-PCR was employed to detect the expression of TUBA4B in GC tissues, cell lines and plasma. In vitro and in vivo experiments were carried out using colony formation/CCK-8/transwell invasion/cell apoptosis assay and xenograft tumor model, respectively. mRNA sequencing was used to identify the TUBA4B-related downstream genes.

Results
TUBA4B was significantly decreased in GC tissues, cells and plasma. Low TUBA4B was positively correlated with larger tumor size, lymph node metastasis and advanced TNM stage. Moreover, TUBA4B was identified as an effective biomarker for the diagnosis and prognosis of patients with GC. Functionally, ectopic expression of TUBA4B inhibited GC cell proliferation, invasion and induced apoptosis in vitro as well as dampened tumor growth and metastasis in vivo. Furthermore, TUBA4B was found to be a competitive endogenous RNA (ceRNA) that could physically bind to and sequester miR-214 and miR-216a/b to increase the expression of their common downstream target PTEN, resulting in inactivation of PI3K/AKT signaling pathway, thereby retarding GC progression.

Conclusion
Our data highlight the compelling regulatory role of TUBA4B in GC, and reactivation of TUBA4B may be a promising therapeutic avenue for GC patients.
Suicidal behaviour among persons with attention-deficit hyperactivity disorder
Authors: Fitzgerald Cecilie , Dalsgaard Søren , Nordentoft Merete , Erlangsen Annette 
Published: June 2019

BackgroundPersons diagnosed with attention-deficit hyperactivity disorder (ADHD) have been found to have an increased risk of suicidal behaviour, but the pathway remains to be thoroughly explored.AimsTo determine whether persons with ADHD are more likely to present with suicidal behaviour (i.e. suicide attempts and deaths by suicide) if they have a comorbid psychiatric disorder.MethodUsing nationwide registers covering the entire population of Denmark, this cohort study of 2.9 million individuals followed from 1 January 1995 until 31 December 2014, covers more than 46 million person-years. All persons aged ≥10 years with Danish-born parents were identified and persons with a diagnosis of ADHD were compared with persons without. Incidence rate ratios (IRRs) were calculated by Poisson regression, with adjustments for sociodemographics and parental suicidal behaviour.ResultsPersons with ADHD were followed for 164 113 person-years and 697 suicidal outcomes were observed. This group was found to have an IRR of suicidal behaviour of 4.7 (95% CI, 4.3–5.1) compared with those without ADHD. Persons with ADHD only had a 4.1-fold higher rate (95% CI, 3.5–4.7) when compared with those without any psychiatric diagnoses. For persons with ADHD and comorbid disorders the IRR was higher yet (IRR: 10.4; 95% CI, 9.5–11.4).ConclusionsThis study underlines the link between ADHD and an elevated rate of suicidal behaviour, which is significantly elevated by comorbid psychiatric disorders. In sum, these results suggest that persons with ADHD and comorbid psychiatric disorders are targets for suicide preventive interventions.Declaration of interestNone.
Water- and Thermal-Stable Silver-Based Photoluminescent Metal-Organic Coordination Polymer for Highly Selective Lead Ion Sensing
Authors: Van Nguyen Chi , Chiang Wei-Hung , Wu Kevin C-W 
Published: June 2019

Abstract
Photoluminescent metal-organic coordination polymers (PLMOCPs) have recently become an attractive material for sensing applications, however, fundamental study of PLMOCP for selective metal ion sensing is still lacking. Here, we report a highly water-stable silver-based photoluminescent metal-organic coordination polymer (PLMOCP) of Ag(BPY)(IPA-NH2) with 404 nm emission as a single probe for selectively detecting Pb2+ in aqueous solutions. The as-synthesized Ag(BPY)(IPA-NH2) was characterized with a high yield, well-defined crystalline structure, extraordinary water and thermal stabilities, and unique optical properties. A high quenching constant of 2*10^4 M−1 and low limit of detection (LOD) of 2.1 µM are determined. Systematic absorbance and PL spectroscopic studies suggest that the PL quenching caused by Pb2+ is the simultaneous dynamic quenching mechanism.
Epidemiology of erectile dysfunction: A cross-sectional web-based survey conducted in an Indonesian national referral hospital
Authors: Birowo Ponco , Deswanto Isaac Ardianson , Rasyid Nur 
Published: June 2019

Background: Many epidemiological studies have demonstrated a high prevalence of erectile dysfunction (ED) in different parts of the world. The objective of the present study was to establish the prevalence of ED in a healthy population from Indonesia and risk factors associated with ED. Methods: We performed a cross-sectional study to estimate the prevalence of erectile dysfunction in relatively healthy males in Jakarta that aged 20 to 80 years old. This cross-sectional study utilizes a web-based survey containing a translated version of The International Index of Erectile Function (IIEF-5) in Indonesian. Sexual domain functions in the IIEF-5 include 4 domains of erectile function starting from erection confidence, erection firmness, erection maintenance and sexual satisfaction. Results: The mean age of respondents is 38.7±12.6 years old. Most of the respondents were married (77.2%), had completed or undertaking tertiary education (66.3%) and worked in privately established companies (35.7%). The prevalence of ED was 35.6% (22.3% mild, 13.7% mild to moderate, 3.1% moderate and 0.8% severe). The prevalence of ED ranges from 6.5% in the 20-29 year old group, to as high as 88.0% in respondents aged 60 years old and above. Age, hypertension, stroke, history of heart disease, diabetes, kidney disease, history of prostate operations and interpersonal stress are significantly associated with ED (p-value = <0.001, <0.001, 0.015 0.000, 0.01, 0.002, <0.001 and 0.022 respectively). Conclusion: The prevalence of ED in Indonesia is about 35.6%. The prevalence of ED in this study ranges from 6.5% to as high as 88.0%. Age, hypertension, stroke, history of heart disease, diabetes, kidney disease, history of prostate operation and interpersonal stress are significantly associated with ED.
Strategic Consumers, Revenue Management, and the Design of Loyalty Programs
Authors: Chun So Yeon , Ovchinnikov Anton 
Published: June 2019

We study the interaction between the design of a premium-status loyalty program, revenue management, and strategic consumer behavior. Specifically, we consider a contemporaneous change where firms across several industries switch their loyalty programs from quantity-based toward spending-based designs. This change has been met with fierce opposition from the media and consumers. Building on the microfoundations of strategic, forward-looking, and status-seeking consumer behavior, we endogenize strategic consumer response to firms’ pricing and loyalty program design decisions, and we characterize conditions under which, by coordinating these decisions, firms can benefit from strategic consumer behavior. We further show that by switching to a spending-based design, firms can benefit from strategic behavior even more, under broader conditions, and in a Pareto-improving way. Finally, we also analyze combined designs, which utilize a combination of quantity and/or spending requirements, and show how they can be used to better manage the transition toward spending-based designs, possibly minimizing negative consumer reactions. This paper was accepted by Serguei Netessine, operations management.
Computer Self-Efficacy Perceptions of Music Teacher Candidates and Their Attitudes towards Digital Technology
Authors: Gudek Bahar 
Published: June 2019

<p style="text-align:justify">The aim of this study was to investigate the attitudes of music teacher candidates on computer self-efficacy perceptions and digital technology according to different variables. In this research, correlational survey model is used as a general survey model and also during data collection process of research, criterion sampling is used as purposive sampling. The focus group of the research consists of 102 students studying music education at Ondokuz Mayis University in the 2018-2019 academic year. Computer Self-Efficacy Perception Scale and Attitude Scale towards Digital Technology Scale were used as data collection tools for the study. According to the results of the study, it was found that there was a significant relationship between the levels of computer self-efficacy perception and the attitudes towards digital technology of the music teacher candidates who participated in the research. The self-efficacy perceptions of the students significantly differed according to their gender, to have a personal computer and the time they first used the computer for educational purpose and their attitudes towards digital technology significantly differed according to their gender, class level and computer ownership.</p>