bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression profiling of the mature C. elegans nervous system by single-cell RNA-Sequencing
Authors: Taylor Seth R , Santpere Gabriel , Reilly Molly , Glenwinkel Lori , Poff Abigail , McWhirter Rebecca , Xu Chuan , Weinreb Alexis , Basavaraju Manasa , Cook Steven J , Barrett Alec , Abrams Alexander , Vidal Berta , Cros Cyril , Rafi Ibnul , Sestan Nenad , Hammarlund Marc , Hobert Oliver , Miller David M. 
Published: August 2019

AbstractA single neuron and its synapses define the fundamental structural motif of the brain but the underlying gene expression programs that specify individual neuron types are poorly understood. To address this question in a model organism, we have produced a gene expression profile of >90% of the individual neuron classes in the C. elegans nervous system, an ensemble of neurons for which both the anatomy and connectivity are uniquely defined at single cell resolution. We generated single cell transcriptomes for 52,412 neurons that resolve as clusters corresponding to 109 of the canonical 118 neuron classes in the mature hermaphrodite nervous system. Detailed analysis revealed molecular signatures that further subdivide identified classes into specific neuronal subtypes. Notably, neuropeptide-related genes are often differentially expressed between subtypes of the given neuron class which points to distinct functional characteristics. All of these data are publicly available at our website (http://www.cengen.org) and can be interrogated at the web application SCeNGEA (https://cengen.shinyapps.io/SCeNGEA). We expect that this gene expression catalog will spur the goal of delineating the underlying mechanisms that define the developmental lineage, detailed anatomy, synaptic connectivity and function of each type of C. elegans neuron.
Impact of serum calcium levels on local and total body bone mineral density: A Mendelian randomization study and an age stratum analysis
Authors: Sun Jing-yi , Zhang Haihua , Zhang Yan , Wang Longcai , Oh Jin Rok , Sun Bao-liang , Liu Guiyou 
Published: August 2019

AbstractObjectivesUntil recently, randomized controlled trials and meta-analyses have not demonstrated convincing conclusions regarding the association of calcium intake with bone mineral density (BMD). Until now, it remains unclear whether high serum calcium levels are causally associated with BMD. This study aimed to investigate the genetic association between serum calcium levels and BMD using a large-scale serum calcium GWAS dataset and four large-scale BMD GWAS datasets in individuals of European descent.MethodsWe performed a Mendelian randomization study to investigate the association of increased serum calcium levels with BMD using a large-scale serum calcium genome-wide association study (GWAS) dataset (including up to 61,079 individuals) and four large-scale BMD GWAS datasets (including minimum 4,180 individuals and maximum 142,487 individuals) regarding the total body, forearm, femoral neck, lumbar spine, and heel BMD. Here, we selected three Mendelian randomization methods including inverse-variance weighted meta-analysis (IVW), weighted median, and MR-Egger.ResultsIn specific site analysis, we found that increased serum calcium levels could reduce BMD at forearm (OR=0.59, 95% CI: 0.36-0.95, P=0.029) and lumbar spine (OR=0.65, 95% CI: 0.49-0.86, P=0.002). We did not identify any suggestive association of genetically increased serum calcium levels with BMD of total body, femoral neck, and heel BMD. In specific age stratum analysis, we found that genetically increased serum calcium levels were statistically significantly associated with reduced total body BMD in age stratum 60 or more years (OR=0.58, 95% CI: 0.41-0.82, P=0.002).ConclusionsWe provide genetic evidence that increased serum calcium levels could not improve BMD in the general population. The elevated serum calcium levels in generally healthy populations, especially adults older than 60 years, may even reduce the BMD, and further cause osteoporosis.
Comparative analysis reveals adaptive evolution of bat IFITMs and a novel antiviral determinant
Authors: Benfield Camilla T O , MacKenzie Farrell , Ritzefeld Markus , Mazzon Michela , Weston Stuart , Tate Edward , Han Teo Boon , Smith Sarah E , Kellam Paul , Holmes Edward C , Marsh Mark 
Published: August 2019

ABSTRACTHost interferon-induced transmembrane proteins (IFITMs) are broad-spectrum antiviral restriction factors. Of these, IFITM3 potently inhibits viruses that enter cells through acidic endosomes, many of which are zoonotic and emerging viruses with bats (order Chiroptera) as natural hosts. We previously demonstrated that microbat IFITM3 is antiviral. Here we show that bat IFITMs are characterized by strong adaptive evolution and identify a highly variable and functionally important site - codon 70 - within the conserved CD225 domain of IFITMs. Mutation of this residue in microbat IFITM3 impairs restriction of four different virus families that enter cells via endosomes. This mutant shows altered subcellular localization and reduced S-palmitoylation, a phenotype copied by mutation of conserved cysteine residues in microbat IFITM3. Furthermore, we show that microbat IFITM3 is S-palmitoylated on cysteine residues C71, C72 and C105, mutation of each cysteine residue individually impairs virus restriction, and a triple C71-C72-C105 mutant loses all restriction, concomitant with subcellular re-localization of microbat IFITM3 to Golgi-associated sites. Thus, we propose that S-palmitoylation is critical for Chiropteran IFITM3 function and identify a key molecular determinant of IFITM3 S-palmitoylation.
A modular cloning toolkit for genome editing in plants
Authors: Hahn Florian , Korolev Andrey , Loures Laura Sanjurjo , Nekrasov Vladimir 
Published: August 2019

AbstractBackgroundCRISPR/Cas has recently become a widely used genome editing tool in various organisms, including plants. Applying CRISPR/Cas often requires delivering multiple expression units into plant and hence there is a need for a quick and easy cloning procedure. The modular cloning (MoClo), based on the Golden Gate (GG) method, has enabled development of cloning systems with standardised genetic parts, e.g. promoters, coding sequences or terminators, that can be easily interchanged and assembled into expression units, which in their own turn can be further assembled into higher order multigene constructs.ResultsHere we present an expanded cloning toolkit that contains ninety-nine modules encoding a variety of CRISPR/Cas-based nucleases and their corresponding guide RNA backbones. Among other components, the toolkit includes a number of promoters that allow expression of CRISPR/Cas nucleases (or any other coding sequences) and their guide RNAs in monocots and dicots. As part of the toolkit, we present a set of modules that enable quick and facile assembly of tRNA-sgRNA polycistronic units without a PCR step involved. We also demonstrate that our tRNA-sgRNA system is functional in wheat protoplasts.ConclusionsWe believe the presented CRISPR/Cas toolkit is a great resource that will contribute towards wider adoption of the CRISPR/Cas genome editing technology and modular cloning by researchers across the plant science community.
Study on the interfacial bonding behaviour of corroded steel bars and TRC constrained concrete
Authors: Hu Chang-Shun , Yin Shiping , Yin Meng-Ti 
Published: August 2019

Purpose
This paper aims to evaluate the bonding properties of textile reinforced concrete (TRC)-confined concrete and corroded plain round bars.

Design/methodology/approach
The bonding performance of three types of specimens (not reinforced, reinforced after corrosion and reinforced before corrosion) was studied by a central pull out test.

Findings
The ultimate bond strength between the corroded steel bars and the concrete is improved when the corrosion ratio is small. After cracking, the degree of corrosion continues to grow and the ultimate bond strength decreases. TRC reinforcement has no detectable effect on the interfacial bonding properties between concrete and plain round bars when the corrosion of steel bars is small; however, when the concrete cracks under the action of rust corrosion, the TRC constraints can effectively improve the bonding performance of the two components.

Practical implications
TRC layer significantly delayed the chloride penetration rate, which can effectively limit the development of corrosion cracking.
Sanitary and phytosanitary regulations and international red meat trade
Authors: Shang Xia , Tonsor Glynn T. 
Published: August 2019

Purpose
The purpose of this paper is to provide an ex post econometric examination of SPS measures and their influences on red meat trade.

Design/methodology/approach
The authors conduct multiple new assessments to further assess the particular effects of specific SPS measures related to animal health, human health and maximum residue limits on red meat trade values. This finer assessment provides updated and more detailed insights into the marginal trade impacts of different SPS measures.

Findings
The current study sheds important light on the determinants of red meat trade. The economic conditions of destination countries and production capability of suppliers are key to determining trade values. Factors including personal income and exporters’ meat supply are identified as trade facilitators. Since the restrictiveness of SPS measures vary across beef and pork sectors, maintaining commodity-specific SPS measures is essential for accurate assessment of trade determinants.

Originality/value
This paper provides multiple contributions to the existing literature and more broadly the authors’ economic understanding on the increasingly contentious issue of global meat trade. Combined, this study yields several implications for food policy, trade negotiators and industry leaders given the growing role and surrounding controversies of trade in meat and livestock markets around the world. The authors further believe the paper would be of notable interest to fellow researchers consistent with the existence of a sizable published literature and ongoing debates in international meat trade.
Consumer behaviour towards suboptimal food products: a strategy for food waste reduction
Authors: Stangherlin Isadora do Carmo , Duarte Ribeiro Jose Luis , Barcellos Marcia 
Published: August 2019

Purpose
Food waste has received attention during the last decade, especially due to its environmental and social impacts. An important contributor to food waste is consumers’ low preference for purchase fruits and vegetables with unusual appearance, products with damaged package and products close to the expiration date, technically called suboptimal food products. Researches show that consumers tend to reject these products when buying food, increasing avoidable food waste. However, consumer considerations when deciding to buy or not to buy suboptimal food are still unknown. The purpose of this paper is to use two different approaches to investigate consumers’ perceptions towards suboptimal food and how they impact their acceptance.

Design/methodology/approach
The first part of the study involved a qualitative analysis of participants’ open-ended responses (282 answers), where participants were asked to write down the impressions they had about three suboptimal food images. The second phase explored consumers acceptance of suboptimal food through a focus group discussion.

Findings
Results reveal that considerations about suboptimal food are divergent, with some participants rejecting them because they are impelled to search for perfection when buying food products. However, some individuals are disposed to accept suboptimal products, mainly because they have concern with the environment and cook abilities.

Originality/value
As a whole, this study contributes for food waste reduction strategies and has implications for marketing actions.
Transforming wood energy in Sweden and Chile
Authors: Alarcón Cristián 
Published: August 2019

Purpose
The purpose of this paper is to critically analyse and problematize the relations between international forestry companies and wood energy in the context of climate change in Chile and Sweden.

Design/methodology/approach
Based on interviews, field observations and analysis of documents, case studies of international forestry companies and wood energy in local areas of Chile and Sweden are examined comparatively. A conceptual framework combining political ecology and environmental communication is developed to approach the cases.

Findings
The paper finds that the two international forestry companies studied here have widely incorporated the use of wood energy as a renewable and carbon neutral energy strategy for their forestry business. Second, the paper finds that wood energy is used as a way to reproduce forestry development in the two countries, which is contested by NGOs and activists which are today articulating critical approaches to forestry development in the two countries. Third, related to the former finding, the paper finds that the incorporation of wood energy into the forest sector’s interests in Chile and Sweden takes place in the context of important social-ecological conflicts related to industrial forestry development.

Originality/value
The paper’s analytical framework helps to analyse the social-ecological nature of international business and the way they organise material practices and communicative meaning around renewable energy. The paper’s findings and analysis shed light on important problematic aspects of the material and symbolic struggles around renewable energy in the context of climate change. The comparative dimension of the analysis has the value to offer a cross-border analysis to improve the understanding of some of the most important aspects of international businesses concerning wood energy today.
Improving work-life policy and practice with an intersectionality lens
Authors: Ryan Ann Marie , Briggs Caitlin Q. 
Published: August 2019

PurposeWork-life research has been critiqued for focusing on the experiences of middle and upper class, younger, White, western and heterosexual women. The purpose of this paper is to provide a critical lens to conceptualizations that take an intersectionality approach, or at least consider multiple identities, in examining work-life conflict and balance.Design/methodology/approachA brief review of the current status of intersectionality research within the work-life realm is provided before discussing the implications of primarily using a single-identity approach to work-life issues. The advantages and challenges of adopting a multiple identity approach are discussed.FindingsThis paper highlights the problems of a lack of an intersectional focus in terms of unidentified needs, ignored values, unresolved conflicts and unhelpful advice. Tensions inherent in trying to adopt an intersectional perspective when dealing with practice and policy issues, particularly with regard to visibility and authenticity, are noted. The paper concludes with a discussion of how considerations of identity and power in work-family research connect to the broader concept of inclusion in the workplace, noting the possible challenges of stereotyping and ambiguity in doing so.Originality/valueApplying an intersectionality lens to efforts to promote work-life balance in organizations can increase inclusivity, but there are tensions and pitfalls associated with this that are particularly of note for practitioners and policy. A research agenda is outlined as a starting point for addressing these issues.
From Anita Hill to Christine Blasey Ford: a reflection on lessons learned
Authors: Heck Sarah E. 
Published: August 2019

Purpose
The purpose of this paper is to offer a critical reflection on congressional testimony in the #MeToo era from the standpoint of a millennial graduate student.

Design/methodology/approach
This essay is based on observational data from a roundtable discussion between Anita Hill and Kimberlé Crenshaw moderated by Dr Dorothy Roberts and connects to themes in research on sex-based harassment.

Findings
The findings of this essay suggest there is still much work to be done in operationalizing intersectionally in the #Metoo era.

Originality/value
The thoughts and opinions expressed in this essay are the author’s own.
Content-based image retrieval based on combination of texture and colour information extracted in spatial and frequency domains
Authors: Tadi Bani Neda , Fekri-Ershad Shervan 
Published: August 2019

Purpose
Large amount of data are stored in image format. Image retrieval from bulk databases has become a hot research topic. An alternative method for efficient image retrieval is proposed based on a combination of texture and colour information. The main purpose of this paper is to propose a new content based image retrieval approach using combination of color and texture information in spatial and transform domains jointly.

Design/methodology/approach
Various methods are provided for image retrieval, which try to extract the image contents based on texture, colour and shape. The proposed image retrieval method extracts global and local texture and colour information in two spatial and frequency domains. In this way, image is filtered by Gaussian filter, then co-occurrence matrices are made in different directions and the statistical features are extracted. The purpose of this phase is to extract noise-resistant local textures. Then the quantised histogram is produced to extract global colour information in the spatial domain. Also, Gabor filter banks are used to extract local texture features in the frequency domain. After concatenating the extracted features and using the normalised Euclidean criterion, retrieval is performed.

Findings
The performance of the proposed method is evaluated based on the precision, recall and run time measures on the Simplicity database. It is compared with many efficient methods of this field. The comparison results showed that the proposed method provides higher precision than many existing methods.

Originality/value
The comparison results showed that the proposed method provides higher precision than many existing methods. Rotation invariant, scale invariant and low sensitivity to noise are some advantages of the proposed method. The run time of the proposed method is within the usual time frame of algorithms in this domain, which indicates that the proposed method can be used online.
Workplace ostracism and employee creativity: role of defensive silence and psychological empowerment
Authors: Chenji Kalaa , Sode Raghavendra 
Published: August 2019

Purpose
The purpose of this paper is to study the impact of workplace ostracism and defensive silence on employee behavior within an organization. The paper attempts to study the impact of workplace ostracism on employee creativity. The paper also aims to train the employees to withstand the negative effects of workplace ostracism.

Design/methodology/approach
This is an empirical study. Primary data were collected from 454 employees working in the service sector in India. The data were collected from an IT sector unit, an insurance company and a bank. Utmost care was taken in order to select organizations where there was frequent interaction between the supervisors and co-workers in order to measure the relevant variables. Mean, standard deviation and correlation were used for descriptive studies, and structural equation modeling was used to measure several dimensions of the hypothesized model. A bootstrap method is used to measure the impact of workplace ostracism on employee creativity.

Findings
The study found that defensive silence mediates between workplace ostracism and employee creativity and psychological empowerment moderates between workplace ostracism and employee creativity. Workplace ostracism negatively effects employee creativity and positively influences defensive silence. The study reveals the moderating effect of psychological empowerment on workplace ostracism and employee creativity such that low levels of psychological empowerment negatively influences employee creativity and high level of psychological empowerment has a positive effect on employee creativity.

Practical implications
Workplace ostracism is proved to be a stressor. The study contributes to the literature on stressor–strain relationship workplace ostracism and employee creativity. Psychological empowerment has indirect influence on workplace ostracism and employee creativity. Organizations may train employees to combat the negative effects of workplace ostracism through concepts like psychological empowerment.

Originality/value
The study is one of its kind as it aimed to measure the impact of workplace ostracism on employee creativity. Though there are several studies on workplace ostracism, its influence on employee creativity perhaps is covered by the present study.
Quality assurance for private universities in Bangladesh
Authors: Alam Gazi Mahabubul 
Published: August 2019

PurposeCommitment of governments and development partners in achieving both qualitative and quantitative measurements for primary and secondary provisions is the key reason for the development of private higher education (HE) and its rapid expansion in the developing world. A considerable amount of attention towards primary and secondary provisions has produced a large numbers of graduates who are theoretically qualified, but have questionable competences in meeting the needs of the market. This has a significant impact on the quality of HE which is delivered by private and public provisions. The purpose of this paper is to examine quality assurance (QA) mechanism set-up for the private HE sector in Bangladesh.Design/methodology/approachQualitative methods were used because interviewees can express their views in a candid way, with a primary focus on the desired themes. Semi-structured interviews were conducted with personnel from the Ministry of Education, the University Grants Commission, students, staff and management from public and private universities. Documents review and secondary data also supplemented.FindingsThe mechanism set-up for QA is yet to mature. Formal arrangements for the governance and regulatory control of private HE provision in Bangladesh are neither sufficient nor up-to-date to help the sector function effectively. The current rules and regulations are suitable only for public HE, but not for the private sector HE.Originality/valueA number of studies have been conducted in the area of private HE in Bangladesh. Only one of them covers QA, and none covers the impact of governance on QA. In the light of this background, this paper is the first of its kind.