bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reusable Immobilized Lactobacillus futsaii CS3 for Enhanced GABA Synthesis using Low-Cost Substrates in Fermenter-Scale Batch and Fed-Batch Fermentations
Authors: Thongruck Kanokwan , Maneerat Suppasil 
Published: October 2024

On the industrial scale, the cost of commercial culture media for the production of gamma-aminobutyric acid (GABA) is a very important factor. This study employed a low-cost substrates and by-product from agri-food industry for GABA synthesis by Lact. futsaii CS3 at the fermenter-scale. Lact. futsaii CS3 cells were immobilized in 3 % (w/v) sodium alginate and employed for GABA synthesis in the optimal modified MRS medium (3.48 % (w/v) cane sugar, 3.84 % (w/v) tuna condensate waste and 10.77 % (w/v) monosodium glutamate (MSG)) with the initial pH medium of 5, fermentation temperature at 37 °C and agitation speed at 30 rpm. During the 60th h of batch fermentation without pH control, immobilized Lact. futsaii CS3 efficiently transformed MSG into the highest GABA of 19.05 g/L, achieving the volumetric productivity of 0.32 g/L/h with a bioconversion rate of 29.01 %. To further enhance GABA production, MSG (7.5 % w/v) was fed into the fermenter during the 48th h of fed-batch fermentation, aiming to amplify GABA synthesis. The maximum GABA (26.28 g/L) was synthesized at 84 h of fed-batch fermentation, and the volumetric productivity and a bioconversion rate of 0.31 g/L/h and 23.59 %, respectively, were obtained. Fed-batch fermentation significantly outperformed batch fermentation, resulting in a 37.95 % increase in GABA production. Moreover, immobilized Lact. futsaii CS3 cells could be reused in 2 batch cycles. In the 1st reusability, the maximum GABA synthesis reached 25.94 g/L, and the volumetric productivity and a bioconversion rate of 0.31 g/L/h and 23.29 %, respectively, were achieved at 84 h of fed-batch fermentation. While in the 2nd reusability, GABA synthesis decreased to 23.29 g/L, with the volumetric productivity and a bioconversion rate of 0.28 g/L/h and 20.91 %, respectively, at 84 h of fed-batch fermentation.
HIGHLIGHTS

GABA production utilizing agri-food industry by-product.
GABA production was enhanced by cane sugar and tuna condensate waste.
Immobilized futsaii CS3 produced GABA 19.05 g/L (batch).
Immobilized futsaii CS3 produced GABA 26.28 g/L (fed-batch).
Immobilized futsaii CS3 could be reused in 2 batch cycles on GABA production.

GRAPHICAL ABSTRACT
Rapid Method for Simultaneous Determination of γ-Oryzanol Compounds in Rice (Oryza sativa) Grains using UV-Vis Spectroscopy and Chemometrics
Authors: Dewi Alfian Mey Pramita , Setyaningsih Widiastuti , Palma Miguel 
Published: October 2024

Rice (Oryza sativa) contains γ-oryzanol, which consists of 4 main compounds: Cycloartenol ferulate, 2,4-methylenecycloartanyl ferulate, campesterol ferulate, and β-sitosterol ferulate, that contribute to the health benefits of rice. This research aimed to develop a rapid method to determine the 4 major γ-oryzanol compounds in 45 varieties covering pigmented (black and red) and non-pigmented (white) rice grain. The method was developed by integrating UV-Vis spectroscopy with chemometrics, specifically principal component analysis (PCA) and partial least square (PLS) regression. Sampling was performed across the Indonesian archipelago collecting 180 samples, which comprises 60 samples for each type of rice in form of rice husk, bran, whole grain, and polished rice. The results of PCA on the spectroscopic data successfully identified distinction for the 3 types of rice attributable by different type and levels of chemical compound in the grain. White rice exhibited a characteristic absorption at 325 nm while in pigmented (red and black) rice showed a maximum absorbance at 280 nm, indicating the presence of different composition of chemical compounds. A reliable model to predict the 4 major γ-oryzanol compounds was established with R2 calibration, R2 cross-validation, and R2 prediction higher than 0.9 was obtained using the spectroscopic data in the range from 200 to 400 nm. However, the PLS modeling was unsuccessful for red and black rice samples most likely due to the interfering high absorption of red colored compounds. Compared to the existing techniques for analyzing individual compounds of the γ-oryzanol by high-performance liquid chromatography, the newly developed approach using UV-Vis spectroscopy combined with chemometrics is more practical, faster, and cost-efficient and mainly, solvent and residues free.
HIGHLIGHTS

A rapid method was developed to determine 4 major γ-oryzanol compounds in rice.
This method integrates UV-Vis spectroscopy with PCA and PLS regression chemometrics.
PCA successfully identified the distinction among white, red, and black rice types.
Reliable PLS models were established for white rice with R2 values above 0.9.
The developed approach is more practical, faster, and residue-free than HPLC.

GRAPHICAL ABSTRACT
Structural Stability of Vacancy and Substitutional Defects in g-GaN: A First-Principles Study
Authors: Fadlliyana Malika , Fajariah Nurul , Nurwantoro Pekik , Prayogi Harmon , Purnawati Diki , Oktavina Cristina Wulan , Nugraheni Ari Dwi , Sholihun Sholihun 
Published: October 2024

Herein, the first-principles calculations of defected monolayer graphene-like gallium nitride (g-GaN) were elucidated. The optimized geometric revealed that the nearest-neighbor distances of N-N (between N atoms) and Ga-Ga (between Ga atoms) were narrowing in most configurations. Interestingly, the VGa has a similar atomic symmetry as the pure g-GaN, which is D3h. Most defected configurations are degraded into C2V symmetries, while the Stone-Wales configuration has the lowest symmetry of CS. The formation energies of V­­Na systems are lower, which implies better energetic stability. For the divacancies, the VGa system is more stable than the V­­Na system. Moreover, the Stone-Wales configuration is energetically more stable than the interchange configuration. Furthermore, the reaction coordinates represent the geometric evolution of each defected system. The result revealed that the N-atoms are consistent with moving outward while the Ga-atoms move inward only for monovacancies and divacancies configurations. In contrast, the N-atoms move inward while the Ga-atoms move outward for substitutions, interchanges, and Stone-Wales configurations. We believe that this finding will be beneficial as the groundwork for future 2D g-GaN-based semiconductor devices.
HIGHLIGHTS

The V­­Na defective system is more stable than the VGa system in monovacancies and substitutions.
The VGaGa defective system is more stable than the V­­Na system in divacancies.
The Stone-Wales system is a stable configuration with a Cs symmetry.

GRAPHICAL ABSTRACT
Application of Mendelian randomization to explore the genetic association between drinking habits of different beverages and sleep disorder
Authors: Zhang Chao , Du Xiaoxun 
Published: October 2024

IntroductionThis study aims to provide genetic evidence linking Drinking Habits of Different Beverages (DHDB) to Sleep Disorder Liability (SDL), potentially informing clinical treatments to improve lifestyle factors.Material and methodsA two-sample Mendelian randomization (MR) method was employed to explore the genetic associations between DHDB and SDL. The DHDB variables studied encompassed Alcohol Intake (ALI), Alcohol Intake Frequency (ALF), High-Frequency Drinking with Meals (HAL), Tea Intake (TEAI), Coffee Intake (COFI), and Red Wine Intake (RWI). The SDL variables analyzed were Insomnia (INS), Inhibit Excitability (IE), and Sleep Duration (SD).ResultsThe MR analysis identified genetic links between ALI and INS, ALF and IE, ALF and SD, HAL and INS, HAL and IE, HAL and SD, TEAI and IE, TEAI and SD, and COFI and IE (P(IVW)<0.05). Nonetheless, no significant genetic links were detected between ALI and IE, ALI and SD, ALF and INS, TEAI and INS, COFI and INS, COFI and SD, RWI and INS, RWI and IE, or RWI and SD (P(IVW)>0.05).ConclusionsAlcohol intake may elevate insomnia risk without affecting daytime sleepiness or sleep duration. Frequent alcohol consumption may lead to daytime sleepiness and reduced sleep duration but not insomnia. Alcohol with meals might reduce insomnia and daytime sleepiness while improving sleep duration. Tea appears unlinked to insomnia and may reduce daytime drowsiness. Coffee may alleviate daytime drowsiness without causing insomnia, and red wine shows no significant association with sleep disorders. Reverse MR suggests potential links between sleep disorders and alcohol or caffeine intake.
High Levels of Uric Acid Upregulate Endothelin Receptors: The Role of MAPK Pathways in an in vitro Study
Authors: Han Yu-jiao , Li Sen 
Published: October 2024

IntroductionUric acid (UA) is the end product of purine compounds' metabolism. There is overwhelming evidence linking hyperuricemia (high levels of UA) and cerebrovascular diseases, but the effect of high levels of UA on cerebral vessels is not fully understood. The aim of this research is to clarify how UA affect the endothelin (ET) receptor in rat cerebral arteries and the related mechanism.Material and methodsIn an in vitro setting, segments of rat cerebral arteries (n=12) were exposed to high levels of UA, either alone or in conjunction with MAPK pathway inhibitors. ET agonists were used to induce contractions that were then measured with a myograph. ET receptor expression was measured using RT-PCR (n=6), Western Blot (n=3), or immunohistochemistry (n=3) to quantify mRNA and protein levels.ResultsThe study revealed that high levels of UA notably increase ETA and ETB receptor-induced contractions and boost the expression of ET receptors in cerebral arteries when compared to that are fresh or cultured alone, suggesting that UA enhances ETA and ETB receptors. Additionally, the up-regulation of ETB receptors induced by UA was inhibited by the p38 inhibitor SB203580, the JNK inhibitor SP600125, and the ERK1/2 inhibitor U0126. SB203580 significantly blocked the increase in ETA receptor-mediated contractions induced by UA and the upregulation of ETA receptor. Neither SP600125 nor U0126 had such effect.ConclusionsHigh levels of UA stimulate the up-regulation of ET receptors in rat cerebral arteries in vitro through MAPK pathways. This study may offer novel perspectives on hyperuricemia-associated cerebrovascular diseases.
Inflammation response index (SIRI) is associated with all-cause and cardiovascular mortality in maintenance hemodialysis patients
Authors: Shi Qiuhong , Lv Bo , Feng Sheng , Jiang Shan , Zeng Ying , Song Kai 
Published: October 2024

IntroductionThe aim of this study was to investigate the association between inflammation response index (SIRI) on all-cause and cardiovascular (CVD) mortality in maintenance hemodialysis (MHD) patients.Material and methods371 MHD patients were included in this retrospective study. Time-dependent receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive value of SIRI. Patients were categorized into two groups based on the median value of SIRI. Kaplan-Meier survival analysis was used to compare the difference in survival rate. Cox regression analysis was used to analyze all-cause and CVD mortality risk factors, and nomograms were established.ResultsThe average age of the patients was (60.71±15.34), and the median follow-up was 42 months. 157 patients died. SIRI had a predictive value for all-cause and CVD mortality. The AUC of the SIRI to predict the all-cause mortality was 0.698, 0.664, 0.713, and 0.900 at 1, 3, 5, and 10 years, respectively. For CVD mortality, the AUC of the SIRI was 0.678, 0.667, 0.717, and 0.906 at 1, 3, 5, and 10 years, respectively. Kaplan-Meier survival analysis showed that patients with high SIRI levels show a significantly lower survival rate. Multivariate Cox regression analysis showed that SIRI > 1.88 was an independent risk factor for all-cause and CVD mortality.ConclusionsSIRI can independently predict all-cause and CVD mortality in MHD patients, which has important value for prognosis.
Cellular therapy for traumatic brain injury in adults: a meta-analysis of controlled clinical trials
Authors: Alizada Mujahid --- , Lin Shu , Gao Hongzhi , Wu Jin-xiang , Alizada Mirwais , Mbori Ngwayi James Reeves , Alizada Muhebullah 
Published: October 2024

IntroductionTraumatic brain injury (TBI) has got no effective treatment. Cellular therapy is the transfer of autologous or allogeneic cells or cellular material into patient(s) for treatment, showed better outcomes in TBI in several clinical and preclinical studies. We performed a meta-analysis on published articles on the topic of cellular therapy for the treatment of TBI in adult patients.Material and MethodsThe literature search was done via selected database with no restrictions on publication year. Studies were included based on selection criteria and quality assessment. The following data were extracted from included articles; author names, publication year and place, type of study, number, sex and age of participants, type of cells used, and post-treatment follow up. The required data related to Fugl-Meyer Motor Scale (FMMS), Disability Rating Scale (DRS) and patients. Overall improvement was pooled and analyzed using RevMan (Ver.5.4.1).ResultsFive studies that met the selection criteria and considered as high quality, containing 367 participants, with an average follow up time of (7.58±6.93) months, were included in meta-analysis. The results showed that cellular therapy significantly improves (OR=0.26; 95% CI=0.15 to 0.48; P=0.0001) the overall performance of the patients. Moreover, the improvements in FMMS (MD=3.79; 95% CI= -2.53 to 10.10; P=0.24) and DRS (MD= -0.16; 95% CI= -1.51 to 1.19; P=0.82) were not statically significant, but they are obviously clinically significant.ConclusionsThis meta-analysis suggests that cellular therapy improves the condition of TBI patients in clinic. Larger, multi-central trials are required to further confirm and detail the use of stem cells in TBI.
A IMPORTÂNCIA DO ALONGAMENTO ESTÁTICO E DINÂMICO NA RECUPERAÇÃO DO ARCO DE MOVIMENTO DE FLEXÃO DE COXOFEMORAL PÓS FRATURA DE COLO DE FÊMUR EM IDOSOS
Authors: Souza Gabriel Antônio de , Januário Júnior Ivo Gabriel , Conceição Karolline Cristina da , Mendonça Michelle Cristina de Souza de , Reis Thayane Marlene Ribeiro , Tuza Fabio Augusto D' Alegria , Azevedo Elaine Aparecida Pedrozo , Portella Claúdio Elídio 
Published: October 2024

Introdução: As fraturas de fêmur no Brasil são frequentes na população idosa. Isso está ligado diretamente ao processo de envelhecimento populacional e afeta a qualidade de vida do indivíduo, como perda de autonomia, dependência funcional e problemas de ordem social e econômica. A fisioterapia no pós-operatório de fratura de fêmur é de extrema importância e necessária para esses pacientes reaprenderem a coordenar seu equilíbrio estático, dinâmico e postural, como a transferência de peso durante a deambulação, iniciando assim o ciclo da marcha com o mínimo de esforço energético e o máximo de eficiência corporal durante o deslocamento. Objetivou-se, por meio deste estudo, comparar o efeito de duas técnicas de alongamento muscular (balístico e estático) no ganho imediato de flexibilidade e melhorar ADM dos músculos. Objetivo: Analisar a efetividade Dos alongamentos estático e dinâmico na recuperação do arco de movimento de coxofemoral pós fratura de fêmur. Métodos: Revisão integrativa da literatura de caráter descritivo e analítico. Este trabalho de pesquisa utilizou as bases de dados da plataforma GOOGLE ACADÊMICO, SCIELO, BVS. Os idiomas usados foram inglês e português. No período de 2014 a 2024. Resultados: A avaliação dos artigos resultou na seleção final de 8 trabalhos, após descartar 87 dos 105 inicialmente obtidos. Entre os artigos aproveitados, 5 (27,77%) mencionaram o alongamento dinâmico e 10 (55,55%) referiram o alongamento estático foram selecionados para compor este estudo. Conclusão: O alongamento dinâmico mostrou mais benéfico na reabilitação de idosos pós fratura de colo de fêmur, pois proporciona menor desconforto e apresenta aumento da ADM articular ao se tratar de flexibilidade. A contribuição verificada pode ser considerada importante para a realização de AVDs
PELATIHAN PEMANFAATAN BAHAN BEKAS SEBAGAI MEDIA PEMBELAJARAN BAGI GURU DI PAUD AN’NASAR
Authors: Risnajayanti Risnajayanti , Usman Usman , Hasan Saali Yunissyah , Selviani Nagandi , Riani Riani 
Published: October 2024

Pengabdian masyarakat ini bertujuan untuk meningkatkan keterampilan guru, serta memanfaatkan bahan bekas sebagai media pembelajaran yang dibentuk secara kreatif. Program pengabdian masyarakat ini melibatkan guru untuk memudahkan pelaksanaan pembelajaran secara efektif dan berkualitas. Penggunaan bahan bekas untuk membuat media pembelajaran, yaitu: stik es krim, botol minuman, kardus, kertas, dan lain-lain. Bahan bekas ini digunakan untuk menyediakan media pembelajaran yang dapat digunakan sesuai dengan kebutuhan guru dan peserta didik dilingkungan sekitar sekolah. Untuk itu, adanya kreativitas guru dalam pembelajaran dengan memanfaat bahan bekas. Bahan bekas merupakan bahan yang sudah tidak terpakai, dan dapat ditemui dilingkungan sekitar yang terkadang hanya menumpuk dan seringkali dibuang ataupun dibakar. Adapun manfaat bahan bekas, yaitu pengolahan atau daur ulang seperti membuat kardus dengan bentuk geometri, stik es krim yang ditempelkan angka, botol minuman yang dibuat dengan bowling huruf, tutup botol yang diberi warna secara bervariasi, dan gelas minuman yang dijadikan wadah untuk memasukan angka atau huruf. Evaluasi yang dilakukan untuk mengevaluasi pengetahuan serta keterampilan guru terkait pelatihan pembuatan media pembelajaran dengan menggunakan bahan bekas setelah program pengabdian masyarakat dilaksanakan. Hasil Pengabdian Masyarakat ini memberikan manfaat bagi guru dan peserta didik dalam memperoleh pengetahuan dan keterampilan yang dapat diterapkan dalam pembelajaran dengan mengkondisikan lingkungan sekitar. Dengan menggunakan bahan bekas, dapat meningkatkan kreativitas dan keterampilan guru dalam mendesain media pembelajaran secara efektif dan berkualitas.
Bł. Józef i Wiktoria Ulmowie jako wzór odpowiedzialności za drugiego człowieka
Authors: Picur Tomasz 
Published: October 2024

Dnia 10 września 2023 r. w Markowej k. Łańcuta miała miejsce beatyfikacja Józefa i Wiktorii Ulmów oraz ich siedmiorga dzieci. Nowi błogosławieni zginęli w czasie II wojny światowej z rąk nazistów za udzielenie Żydom schronienia w swoim domu. Zeznania świadków, jak również zachowane dokumenty i pamiątki jednoznacznie potwierdzają, że ratując ich życie, Ulmowie kierowali się wiarą, a szczególnie ewangelicznym ideałem miłości bliźniego wyrażonym w przypowieści o miłosiernym Samarytaninie. Wynosząc ich do chwały ołtarzy, Kościół pragnie nie tylko docenić ich heroiczną postawę i ofiarę życia, ale również ukazać jako przykład do naśladowania dla współczesnych i przyszłych pokoleń. Wśród wielu cech składających się na duchowo-moralny profil rodziny z Markowej, jednym z najbardziej charakterystycznych rysów jej świętości jest bez wątpienia żywe i głęboko ewangeliczne poczucie odpowiedzialności za bliźniego. Niniejszy artykuł przybliża teologiczno-moralną wizję odpowiedzialności za drugiego człowieka, ukazuje jej realizację w życiu rodziny Ulmów oraz zwraca uwagę na jej aktualizację w kontekście współczesnych wyzwań i problemów.
Zmartwychwstanie Pańskie w pasterskim nauczaniu Abpa Józefa Michalika
Authors: Zawałeń Radosław 
Published: October 2024

Celebrowanie Misterium Paschalnego Jezusa Chrystusa stoi w centrum życia Kościoła. Tym samym przepowiadanie prawdy o wydarzeniach poranka wielkanocnego zdaje się być pierwszą powinnością tych, którzy dzisiaj głoszą Ewangelię. Stojący przed Sanhedrynem apostołowie bez wahania zresztą wyznają: „Bo my nie możemy nie mówić tego, cośmy widzieli i słyszeli” (Dz 4,20). Świadomość zmartwychwstania w pierwotnym Kościele zasadza się na formułach wiary, katechezach paschalnych św. Piotra i św. Pawła. Samo zaś uzasadnienie faktu zmartwychwstania oparte jest na pustym grobie, chrystofaniach paschalnych i ukonstytuowaniu się wspólnoty wyznającej żywą wiarę w zmartwychwstałego Chrystusa. Stąd też zadaniem niniejszego artykułu jest zaprezentowanie pasterskiego nauczania Abpa Józefa Michalika, metropolity przemyskiego w latach 1993-2016, na temat zmartwychwstania Pańskiego, który uwzględnia w swoim przepowiadaniu wiarygodność rezurekcji oraz jej znaczenie w życiu chrześcijańskim.
Jan Tabiński – malarz z Galicji
Authors: Matyaszewska Elżbieta 
Published: October 2024

Niniejszy artykuł poświęcony został Janowi Tabińskiemu, malarzowi kościelnemu, działającemu na terenie Galicji w drugiej połowie XIX i pierwszym dziesięcioleciu XX wieku. Artyście, który był autorem bardzo wielu malowideł ściennych i sztalugowych o tematyce religijnej, wykonywanych dla kościołów ówczesnej archidiecezji lwowskiej, a zwłaszcza diecezji przemyskiej. Zajmował się też restauracją wcześniej powstałych dzieł sztuki, sporadycznie malował też portrety. Jan Tabiński był absolwentem krakowskiej Szkoły Sztuk Pięknych i nie jest wykluczone, że swój artystyczny warsztat doskonalił też w Wiedniu. W początkowym okresie swej działalności pracował przy renowacji i ozdabianiu wnętrz zamku w Łańcucie, później poświęcił się głównie pracy dla Kościoła, malując wnętrza galicyjskich świątyń i wykonując dlań obrazy sakralne, utrzymane w realistycznym stylu. Od 1880 roku na stałe mieszkał w Rzeszowie, gdzie zmarł w wieku 79 lat.
Загадка Саратока: проблемы локализации царства во времени и пространстве
Authors: Анисимов Константин Александрович 
Published: October 2024

В статье рассматривается проблема правления Саратока, фракийского правителя, известного только по монетам. Эти монеты неоднократно привлекали внимание специалистов, благодаря чему был накоплен значительный набор фактов. Однако имеющиеся гипотезы, на наш взгляд, не раскрывают исторического контекста появления этих монет. В рамках работы рассматриваются основные гипотезы локализации его владений и датировки правления. Отвергаются предположения о том, что Сараток был не царем, а могущественным «парадинастом», а также попытка отождествления его со знаменитым царем одрисов Ситалком. На основании исследований нумизматов и данных письменных источников предлагается следующая гипотеза: Сараток – царь одрисов, соправитель Севта I, правивший около 424–407 гг. до н. э. в верхнем течении рек Марица и Тунджа. Исходя из этого, с его правлением связываются важные структурные изменения в Одрисском царстве.
«Уютная катастрофа» в произведениях японских писателей конца XX — начала XXI веков: ужас и эстетизм апокалипсиса
Authors: А. Борькина 
Published: October 2024

В статье рассматривается использование современными японскими писателями мотива «уютной катастрофы», характерного для британской научно-фантастической литературы 50-х годов XX века. В числе его особенностей выделяются ничем не примечательный главный герой; сосредоточенность персонажей, оказавшихся в условиях катастрофы, на тривиальных бытовых вопросах; фокус на «внутреннем пространстве» произведения. Сходные черты наблюдаются и в работах японских писателей, где центральное место занимают бытописание и понятие «персонального пространства». Для японских авторов характерно также изображение эмоционального переживания, часто выраженного категориями традиционной японской эстетики.
The article discusses the usage of “cozy catastrophe” motive, which emerged in British science fiction in 1950s, in the works of contemporary Japanese writers. Its features include an ordinary protagonist; the concentration of the characters, finding themselves in the middle of the catastrophe, on trivial everyday matters; the focus on the “inner space” of the literary work. Similar points can be found in contemporary Japanese fiction, where depiction of ordinary life and “personal space” are considered essential. Japanese authors also pay attention to the emotional experience, which is often expressed through traditional Japanese aesthetic categories.