bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virtual reality objects improve learning efficiency and retention of diagnostic ability in fetal ultrasound
Authors: Ebert J. , Tutschek B. 
Published: July 2018

ABSTRACTObjectiveVirtual reality (VR) objects of fetal ultrasound volumes have been proposed for teaching and learning diagnostic ultrasound. The aim of this study was to determine if VR objects improve learning efficiency and retention of diagnostic ability in fetal ultrasound.MethodsMedical students and junior doctors were taught normal and abnormal sonographic fetal brain anatomy using conventional means (video lectures and review articles; control group) or additionally with selected VR objects from a novel fetal brain atlas (Pocket Brain,
http://pb.fetal.ch; study group). Knowledge, speed of recognition and retention of diagnostic ability were tested in multiple‐choice questionnaires 1 and 4 months after teaching, and the results were compared between those taught using conventional means only and those taught using VR objects.ResultsParticipants taught using VR objects answered significantly more questions correctly and solved the tests quicker than those taught using conventional methods only, both 1 and 4 months after teaching.ConclusionThe use of VR objects in teaching fetal ultrasound significantly improves learning efficiency and knowledge retention. Copyright © 2018 ISUOG. Published by John Wiley & Sons Ltd.
Quantitative systems models illuminate arrhythmia mechanisms in heart failure: Role of the Na+‐Ca2+‐Ca2+/calmodulin‐dependent protein kinase II‐reactive oxygen species feedback
Authors: Morotti Stefano , Grandi Eleonora 
Published: July 2018

Quantitative systems modeling aims to integrate knowledge in different research areas with models describing biological mechanisms and dynamics to gain a better understanding of complex clinical syndromes. Heart failure (HF) is a chronic complex cardiac disease that results from structural or functional disorders impairing the ability of the ventricle to fill with or eject blood. Highly interactive and dynamic changes in mechanical, structural, neurohumoral, metabolic, and electrophysiological properties collectively predispose the failing heart to cardiac arrhythmias, which are responsible for about a half of HF deaths. Multiscale cardiac modeling and simulation integrate structural and functional data from HF experimental models and patients to improve our mechanistic understanding of this complex arrhythmia syndrome. In particular, they allow investigating how disease‐induced remodeling alters the coupling of electrophysiology, Ca2+ and Na+ handling, contraction, and energetics that lead to rhythm derangements. The Ca2+/calmodulin‐dependent protein kinase II, which expression and activity are enhanced in HF, emerges as a critical hub that modulates the feedbacks between these various subsystems and promotes arrhythmogenesis.This article is categorized under:

Physiology > Mammalian Physiology in Health and Disease
Models of Systems Properties and Processes > Mechanistic Models
Models of Systems Properties and Processes > Cellular Models
Models of Systems Properties and Processes > Organ, Tissue, and Physiological Models
Colonic fibromatosis − a case report and review of the literature
Authors: Linardoutsos Dimitrios , Patel Neal , Patel Hitesh 
Published: July 2018

AbstractIntra-abdominal desmoids tumours are very rare and usually occur in patients with familiar adenomatous polyposis and previous surgery. They represent fibroepithelial growths with varied biologic behavior and therefore different prognosis. We report a case of a 60-year-old patient with a large right colonic mass who underwent right hemicolectomy. Histology proved morphological and immuno-histochemical features indicating fibromatosis. This desmoid tumour appeared growing from the colonic wall rather than the mesocolon, confirming a true colonic wall fibromatosis, a deep isolated form of intra-abdominal fibromatosis. Surgical resection is the treatment of choice in isolated well confirmed lesions. Multidisciplinary team approach is crucial for treatment and prognosis.
Migration networks and narratives in Ghana: a case study from the Zongo
Authors: Casentini Giulia 
Published: July 2018

AbstractThe historical presence of Zongo communities in contemporary Ghana is analysed through archival documents and ethnography, with the aim of highlighting their social and political value as migrant communities, and their possible inclusion in the urban strategy of the country. Zongos have been present in Ghana since precolonial and colonial times, depending on specific cases, and are historically connected to the presence of Muslim trade communities in the market areas of various urban settlements. I argue that their role in the Ghanaian socio-political landscape goes beyond this common definition: Zongos act as interlocutors between the urban centre and peripheral rural areas, and they have a potentially effective role in dealing with migrant flows. Zongo people elaborate their memory of migration in particular ways, revealing both an inherent mode of producing a common group identity, and a conscious strategy of inclusion in the contemporary political dynamics of Ghana.
Monetary Union: A Desperate Gamble
Authors: Minford Patrick 
Published: July 2018

Dr Tim Bunch (President, Manchester Actuarial Society): Good afternoon, ladies and gentlemen. Welcome to Manchester Town Hall for this special meeting of the Manchester Actuarial Society, which is being held to commemorate the 150th anniversary of the actuarial profession in the UK.I should like to welcome particularly various guests. There are guests invited by the Manchester Actuarial Society, and also guests of actuarial firms in the north of England. I would particularly like to welcome Paul Thornton, the current President of the Institute.Our speaker today is Professor Patrick Minford, who is Professor of Economics at Cardiff Business School, which is at the University of Wales. He has been in that position since October 1997. Prior to that, he was Professor of Economics at Liverpool University, and he maintains his contacts with the University of Liverpool through being Director of the Liverpool research group in macro-economics. He has held economic positions in a number of places, including HM Treasury, at Manchester University and at the National Institute for Economic and Social Research.
‘I don't want to be, feel old’: older Canadian men's perceptions and experiences of physical activity
Authors: Hurd Clarke Laura , Currie Lauren , Bennett Erica V. 
Published: July 2018

AbstractRelatively few older adults are physically active despite extensive research exploring barriers and facilitators and concomitant interventions designed to enhance participation rates. Building on the growing literature that considers the subjective experience of being physically active, we explored the meanings that older Canadian men attributed to physical activity broadly defined. Thus, we examined their experiences and perceptions of exercise, sport and/or leisure-time physical activities. Data are presented from qualitative interviews with 22 community-dwelling Canadian men aged 67–90. Our analysis resulted in three overarching categories that subsumed the men's understanding of physical activity. ‘I do it for my health’ described how the men stated that their primary reason for engaging in exercise was to maintain their health and body functionality so that they could age well and continue to participate in sport and leisure. ‘It feels good’ referred to the various ways that the men derived pleasure from being active, including the physical sensations, psychological benefits and social connections they derived from their participation. ‘It gets tougher’ detailed the ways that the men were finding physical activity to be increasingly difficult as a result of the onset of health problems, declining body functionality and the social realities of ageing. We discuss our findings in light of the extant literature concerning age relations, ageism, and the third and fourth ages.
Was that my name? Infants’ listening in conversational multi-talker backgrounds
Authors: BERNIER Dana E. , SODERSTROM Melanie 
Published: July 2018

AbstractThis study tested infants’ ability to segregate target speech from a background of ecologically valid multi-talker speech at a 10 dB SNR. Using the Headturn Preference Procedure, 72 English-learning 5-, 9-, and 12-month-old monolinguals were tested on their ability to detect and perceive their own name. At all three ages infants were able to detect the presence of the target speech, but only at 9 months did they show sensitivity to the phonetic details that distinguished their own name from other names. These results extend previous findings on infants’ speech perception in noise to more naturalistic forms of background speech.
Subtractive analysis of S. franciscanus and S. purpuratus ovary mRNA: what kinds of genes determine species-specificity?
Authors: Kamei Noriko , Glabe Charles G. 
Published: July 2018

Our goal is to identify and understand points of regulation in sperm–egg recognition as well as in steps of early development. These processes are species-specific and are the key to understanding speciation. In both vertebrates and invertebrates, the interaction of the sperm and egg displays a wide range of species-specificity. The questions we would like to answer are: What kinds of molecules determine the specificity and control the fertilisation process? Are early steps in development regulated in a species-specific manner?As an approach to identifying genes that determine species-specificity, in two different species, S. purpuratus (S. p.) and S. franciscanus (S. f.), we used a new subtractive hybridisation method known as RDA (representational difference analysis) (Lisitsyn et al., 1993; Hubank et al., 1994). Several species-specific clones were isolated from S. f. ovary mRNA by this method using mRNA from another species but the same genus of sea urchin, S. p. Four different clones were obtained and the species-specificity of the sequence was confirmed by hybridisation. One of them has four tandem EGF repeats and is homologous to the S. p. EGF-II gene (Yang et al., 1989) and A. crassispina EGIP (exogastrula inducing protein) gene (Ishihara et al., 1982). The first three EGF repeats (EGF 1–3) have 60% similarity among these species, but the fourth EGF domain (EGF 4) is highly divergent. The EGF-II protein is believed to be involved in the signalling events of early development, because purified EGF causes exogastrulation when it is added to the seawater prior to gastrulation (Ishihara et al., 1982). Recombinant EGF 3 from S. f. induces exogastrulation in both S. f. and S. p.
Cellular analyses of sea urchin metamorphosis
Authors: Sato Yukiko , Yazaki Ikuko 
Published: July 2018

Larvae of marine invertebrates undergo metamorphosis in response to environmental cues (Chia & Burke, 1978). In sea urchins, free fatty acids (Kitamura et al., 1993), dibromomethane (Taniguchi et al., 1994), pheromonal peptides (Burke, 1984) and L-glutamine (Yazaki & Harashima, 1994; Yazaki, 1995) have been known as metamorphosis-inducing substances. The mechanisms by which cells respond to these cues and how the larval tissues are absorbed have not been clear, however. In the present study, we used L-glutamine (Gln) and a natural cue, green algae (Ulvella sp.), to induce metamorphosis of Hemicentrotus pulcherrimus and Anthocidaris crassispina, and investigated the intracellular changes during metamorphosis.After being subjected to 10−5–10−3 M Gln for 10–24 h, larvae cease swimming, settle, begin to retract their larval arms, extrude the primary podia and finally evert their echinus rudiment (ER). In H. pulcherrimus, larvae retracted their arms from 6 h to 24 h after the start of Gln treatment and then everted the ER. A. crassispina larvae underwent similar processes to those of H. pulcherrimus. The larval surface is composed of squamous epithelium and columnar epithelium. The epithelium of the ciliary bands or epaulets is columnar.In the squamous epithelium, the nuclear chromatin in the larval arms and body, and in the oesophagus, markedly condensed after treatment with Gln for 24 h. Electron microscopy revealed swelling of both nuclei and mitochondria, while their membranes seemed to be intact. In the cytoplasm, lipid-like structures and electron-dense substances appeared. A further 24 h after Gln treatment, the chromatin condensation had progressed. Most nuclei in which chromatin had condensed were positive to the TUNEL assay, which detects DNA fragmentation. These results suggest that cell death in the squamous epithelium is apoptotic rather than necrotic.
Competence of the animal cap to react with the inductive signal from micromere descendants in the hatching blastula stage of echinoid embryos
Authors: Ishizuka Yasuhiro , Amemiya Shonan 
Published: July 2018

Micromere signalling is a key to understanding the developmental mechanisms underlying endomesoderm differentiation along the A-V axis in sea urchin embryos. A recent study has shown that micromere activity in inducing endo-mesoderm differentiation of mesomere descendants is, unexpectedly, maximal at the hatching blastula (HB) stage in the echinoids Scaphechinus mirabilis and Hemicentrotus pulcherrimus. This study focused mainly on the timing of emission of the inductive signal by the micromere
descendants. The timing of animal cap competent to react to the inductive signal from micromeres was not specifically investigated.In the present study, we examined the competence of mesomere descendants at the HB stage to react to the inductive signal of micromere descendants by producing recombinant embryos of the descendants of mesomeres and micromeres.Adults of the sand dollar Scaphechinus mirabilis and the sea urchin Hemicentrotus pulcherrimus were collected along the shore of Shiraishi Island, Okayama Prefecture and in the vicinity of the Misaki Marine Biological Station, Kanagawa Prefecture, respectively. The fertilised eggs were separated into two groups immediately after removal of the fertilisation membranes. One group was cultured in normal artificial seawater (ASW), and the other in ASW containing 50 mg/ml of rhodamine B isothiocyanate (RITC). At the early 16-cell stage unlabelled embryos were transferred to calcium-free seawater (CFSW) and dissected in the equatorial plane with a glass needle to isolate animal caps consisting of eight mesomeres. Embryos labeled with RITC were also transferred to CFSW at the same stage and dissected to isolate four micromeres. An isolated animal cap and a quartet of micromeres were cultured separately in ASW and recombined at the stage corresponding to the HB of control (undisturbed) embryos.
Inversion of left–right asymmetry in the formation of the adult rudiment in sea urchin larvae: removal of a part of embryos at the gastrula stage
Authors: Aihara Mizuki , Amemiya Shonan 
Published: July 2018

At the late pluteus stage, sea urchin larvae form an adult rudiment left–right (LR) asymmetrically, on only the left side. Little is known about how the LR asymmetry of the adult rudiment is established in earlier stages during which the larval body is basically LR symmetric. To investigate how the different regions of the embryo function to establish LR asymmetry, we removed different regions at the gastrula stage and assessed the effects of the operation on the establishment of LR asymmetry of the adult rudiment.Surgery was performed on mid- to late-gastrula embryos of two indirect developing species: Scaphechinus mirabilis and Hemicentrotus pulcherrimus. The embryos were placed under a dissecting microscope (SMZ8, Leica) and ectodermal epithelium together with underlying mesenchyme cells was dissected out with a glass needle. The region along the midline of the embryo at the width of the archenteron was designated the ‘midline region’, and the region lateral to the ‘midline region’ was designated the ‘lateral region’. When the left and/or the right side was excised, the whole lateral region was precisely removed on the animal side, but the plane of incision deviated more laterally on the vegetal side to avoid the ventro-lateral cluster of primary mesenchyme cells, so that a part of the defined ‘lateral region’ was left on the vegetal side. The vertical excision was made by cutting mid-gastrula embryos vertically to the archenteron at a level just superior to the tip of the archenteron. In the sham operation, embryos were pressed with the needle as in the vertical excision, but the incision was stopped slightly before the animal and vegetal halves of the embryos were completely divided from each other. The operated embryos were examined through an optical microscope (Optiphoto, Nikon) to confirm that the excision was correct, and they were then cultured with a food supply (diatoms, Chaetoceros gracilis). The handedness of the adult rudiment was examined at the six-armed or eight-armed pluteus stage through an optical microscope (Table 1).
Analyzing the political survival prospects of Kim Jong-un's North Korean regime through the framework of selectorate theory
Authors: Lee Hanhee 
Published: July 2018

AbstractThe political survival of Kim Jong-un's regime in North Korea has been a major international concern since his accession in 2012. While international research has endeavored to diagnose the stability of the regime based on its limitations and weaknesses due to political, economic, and international factors, studies based on baseline theoretical models of leadership stability have rarely been undertaken. This paper employs selectorate theory to address this issue, by identifying the selectorate and winning coalition in North Korea and illustrating their relationships with Kim Jong-un's political survival. To reexamine the analytical framework and results, this study has undertaken in-depth interviews with high-level North Korean defectors who have served in key power apparatuses of Kim Jong-il and/or Kim Jong-un's regime. It analyzes how frequent co-optation of top aides, economic ideologies, and policies are utilized for political survival. It further explains the correlation of his political survival with the party-dependent bureaucracy, internal reign of terror, development of nuclear capacity, and continuous military provocations. However, as the regime further intensifies the reign of terror, the possibility of acoup d’étatby the selectorate and coalition cannot be completely ruled out.
Breeding for soil-borne pathogen resistance impacts active rhizosphere microbiome of common bean
Authors: Mendes Lucas William , Mendes Rodrigo , Raaijmakers Jos M , Tsai Siu Mui 
Published: July 2018

Abstract
Over the past century, plant breeding programs have substantially improved plant growth and health, but have not yet considered the potential effects on the plant microbiome. Here, we conducted metatranscriptome analysis to determine if and how breeding for resistance of common bean against the root pathogen Fusarium oxysporum (Fox) affected gene expression in the rhizobacterial community. Our data revealed that the microbiome of the Fox-resistant cultivar presented a significantly higher expression of genes associated with nutrient metabolism, motility, chemotaxis, and the biosynthesis of the antifungal compounds phenazine and colicin V. Network analysis further revealed a more complex community for Fox-resistant cultivar and indicated Paenibacillus as a keystone genus in the rhizosphere microbiome. We suggest that resistance breeding in common bean has unintentionally co-selected for plant traits that strengthen the rhizosphere microbiome network structure and enrich for specific beneficial bacterial genera that express antifungal traits involved in plant protection against infections by root pathogens.
Microbial biogeography of 925 geothermal springs in New Zealand
Authors: Power Jean F. , Carere Carlo R. , Lee Charles K. , Wakerley Georgia L. J. , Evans David W. , Button Mathew , White Duncan , Climo Melissa D. , Hinze Annika M. , Morgan Xochitl C. , McDonald Ian R. , Cary S. Craig , Stott Matthew B. 
Published: July 2018

AbstractGeothermal springs are model ecosystems to investigate microbial biogeography as they represent discrete, relatively homogenous habitats, are distributed across multiple geographical scales, span broad geochemical gradients, and have reduced metazoan interactions. Here, we report the largest known consolidated study of geothermal ecosystems to determine factors that influence biogeographical patterns. We measured bacterial and archaeal community composition, 46 physicochemical parameters, and metadata from 925 geothermal springs across New Zealand (13.9–100.6 °C and pH < 1–9.7). We determined that diversity is primarily influenced by pH at temperatures <70 °C; with temperature only having a significant effect for values >70 °C. Further, community dissimilarity increases with geographic distance, with niche selection driving assembly at a localised scale. Surprisingly, two genera (Venenivibrio and Acidithiobacillus) dominated in both average relative abundance (11.2% and 11.1%, respectively) and prevalence (74.2% and 62.9%, respectively). These findings provide an unprecedented insight into ecological behaviour in geothermal springs, and a foundation to improve the characterisation of microbial biogeographical processes.
Middle Eocene greenhouse warming facilitated by diminished weathering feedback
Authors: van der Ploeg Robin , Selby David , Cramwinckel Marlow Julius , Li Yang , Bohaty Steven M. , Middelburg Jack J. , Sluijs Appy 
Published: July 2018

AbstractThe Middle Eocene Climatic Optimum (MECO) represents a ~500-kyr period of global warming ~40 million years ago and is associated with a rise in atmospheric CO2 concentrations, but the cause of this CO2 rise remains enigmatic. Here we show, based on osmium isotope ratios (187Os/188Os) of marine sediments and published records of the carbonate compensation depth (CCD), that the continental silicate weathering response to the inferred CO2 rise and warming was strongly diminished during the MECO—in contrast to expectations from the silicate weathering thermostat hypothesis. We surmise that global early and middle Eocene warmth gradually diminished the weatherability of continental rocks and hence the strength of the silicate weathering feedback, allowing for the prolonged accumulation of volcanic CO2 in the oceans and atmosphere during the MECO. These results are supported by carbon cycle modeling simulations, which highlight the fundamental importance of a variable weathering feedback strength in climate and carbon cycle interactions in Earth’s history.