bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spirituality, infertility‐related stress, and quality of life in Brazilian infertile couples: Analysis using the actor‐partner interdependence mediation model
Authors: Casu Giulia , Ulivi Giulia , Zaia Victor , Fernandes Martins Maria do Carmo , Parente Barbosa Caio , Gremigni Paola 
Published: February 2018

ABSTRACTInfertility has a stressful impact on both partners, with adverse effects on the quality of life of infertile couples. Spirituality is a meaning‐based strategy that can protect couples against infertility's negative impact on quality of life, but analysis of this mediator relationship in infertile couples has not been reported. We adopted a dyadic approach and used the actor‐partner interdependence mediation model to examine whether and how women's and men's spirituality was associated with their own and their partners’ infertility‐related stress and quality of life. In 2014, 152 infertile couples starting their first fertility treatment at a private clinic in Brazil were recruited and completed self‐reports of spirituality, infertility‐related stress, and quality of life. Results indicated that women's and men's level of spirituality was positively associated with their own quality of life directly and indirectly, by reducing their own infertility‐related stress. Their spirituality was associated with an increase in their partners’ quality of life only indirectly, by reducing their partners’ infertility‐related stress. Findings highlight the importance of assessing and promoting spirituality as a coping resource that infertile women and men might use to deal with the stress of infertility and reduce its adverse effects on quality of life.
Ruthenium counterstaining for imaging mass cytometry
Authors: Catena Raúl , Montuenga Luis M , Bodenmiller Bernd 
Published: February 2018

AbstractImaging mass cytometry is a novel imaging modality that enables simultaneous antibody‐based detection of >40 epitopes and molecules in tissue sections at subcellular resolution by the use of isotopically pure metal tags. Essential for any imaging approach in which antigen detection is performed is counterstaining, which reveals the overall structure of the tissue. Counterstaining is necessary because antigens of interest are often present in only a small subset of cells, and the rest of the tissue structures are not visible. As most biological tissues are nearly transparent or non‐fluorescent, chromogenic reagents such as haematoxylin (for immunohistochemistry) or fluorescent dyes such as 4′,6‐diamidino‐2‐phenylindole (which stains nuclei for epifluorescence and confocal microscopy) are utilized. Here, we describe a metal‐based counterstain for imaging mass cytometry based on simple oxidation and subsequent covalent binding of the tissue components to ruthenium tetroxide (RuO4). RuO4 counterstaining reveals general tissue structure both in areas with high cell content and in stromal areas with low cellularity and fibrous or hyaline material in a manner analogous to haematoxylin in immunohistochemical counterstaining or eosin or other anionic dyes in conventional histology. Our new counterstain approach is applicable to any metal‐based imaging technique, and will facilitate the adaptation of imaging mass cytometry for routine applications in clinical and research laboratories. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Impulsive second‐order sliding mode control in reduced information environment
Authors: Aldukali Fathi M. , Shtessel Yuri B. , Plestan Franck 
Published: February 2018

SummaryThe perturbed system with input‐output dynamics of arbitrary and well‐defined relative degree is considered in a reduced information environment. A novel impulsive second‐order sliding mode control in the reduced information environment is proposed. The almost instantaneous convergence to the origin is achieved via impulsive control acting in a concert with second‐order sliding mode control, specifically supertwisting and twisting algorithms. The impulsive actions are implemented in a piecewise constant format. Numerical examples illustrate the efficiency of the proposed control algorithms.
Rapid Osteogenic Enhancement of Stem Cells in Human Bone Marrow Using a Glycogen-Synthease-Kinase-3-Beta Inhibitor Improves Osteogenic Efficacy In Vitro and In Vivo
Authors: Clough Bret H. , Zeitouni Suzanne , Krause Ulf , Chaput Christopher D. , Cross Lauren M. , Gaharwar Akhilesh K. , Gregory Carl A. 
Published: February 2018

Abstract
Non-union defects of bone are a major problem in orthopedics, especially for patients with a low healing capacity. Fixation devices and osteoconductive materials are used to provide a stable environment for osteogenesis and an osteogenic component such as autologous human bone marrow (hBM) is then used, but robust bone formation is contingent on the healing capacity of the patients. A safe and rapid procedure for improvement of the osteoanabolic properties of hBM is, therefore, sought after in the field of orthopedics, especially if it can be performed within the temporal limitations of the surgical procedure, with minimal manipulation, and at point-of-care. One way to achieve this goal is to stimulate canonical Wingless (cWnt) signaling in bone marrow-resident human mesenchymal stem cells (hMSCs), the presumptive precursors of osteoblasts in bone marrow. Herein, we report that the effects of cWnt stimulation can be achieved by transient (1–2 hours) exposure of osteoprogenitors to the GSK3β-inhibitor (2′Z,3′E)-6-bromoindirubin-3′-oxime (BIO) at a concentration of 800 nM. Very-rapid-exposure-to-BIO (VRE-BIO) on either hMSCs or whole hBM resulted in the long-term establishment of an osteogenic phenotype associated with accelerated alkaline phosphatase activity and enhanced transcription of the master regulator of osteogenesis, Runx2. When VRE-BIO treated hBM was tested in a rat spinal fusion model, VRE-BIO caused the formation of a denser, stiffer, fusion mass as compared with vehicle treated hBM. Collectively, these data indicate that the VRE-BIO procedure may represent a rapid, safe, and point-of-care strategy for the osteogenic enhancement of autologous hBM for use in clinical orthopedic procedures.
Copper(II) Complex Supported on Fe3O4@SiO2 Coated by Polyvinyl Alcohol as Reusable Nanocatalyst in N‐Arylation of Amines and N(H)‐ Heterocycles and Green Synthesis of 1H‐Tetrazoles
Authors: Sardarian Ali Reza , Eslahi Hassan , Esmaeilpour Mohsen 
Published: February 2018

AbstractIn this paper, copper(II) based catalyst bearing polyvinyl alcohol immobilized on Fe3O4@SiO2 was synthesized and fully characterized by FT‐IR, XRD, TEM, FE‐SEM, DLS, UV‐Vis, XPS, EDX, TGA, VSM, NMR, ICP and elemental analysis techniques. Then, the performance of this magnetic heterogeneous system was investigated in the N‐arylation of nitrogen heterocycles and alkyl amines with aryl halides to produce the desired coupled products in good to excellent yields without using external ligands or additives as promoters. Also, this reusable copper based magnetic nanocatalyst was employed in one‐pot three component and green synthesis of 5‐substituted 1H‐tetrazoles in excellent yields through the click reaction of aliphatic and aromatic aldehydes with hydroxylamine hydrochloride and azide salt. This catalytic system was easily recovered by a magnetic field and reused for at least 7 times without distinct deterioration in catalytic activity. Also, the analysis of the reaction mixture by the ICP technique showed that the leaching of the catalyst was negligible.
Role of Electronegative Atom Present on Ligand Backbone and Substrate Binding Mode on Catecholase‐ and Phosphatase‐Like Activities of Dinuclear NiII Complexes: A Theoretical Support
Authors: Adhikary Jaydeep , Majumdar Ishani , Kundu Priyanka , Kornweitz Haya , Kara Hulya , Das Debasis 
Published: February 2018

AbstractThe reaction of two pentadentate compartmental ligands HL1 and HL2 [ HL1=2,6‐bis((E)‐(2‐morpholinoethylimino)methyl)‐4‐tert‐butylphenol; HL2=2,6‐bis((E)‐(2‐(piperidin‐1‐yl)ethylimino)methyl)‐4‐tert‐butylphenol] with nickel acetate followed by addition of NaSCN afforded two discrete dinuclear complexes, [Ni2L1(CH3COO)2(SCN)].(H2O)2.(0.5CH3OH) (1) and [Ni2L2(CH3COO)(SCN)2(CH3OH)].(CH3OH) (2). Single crystal structure reveals that the complexes are NiII dimer with triple‐mixed phenoxo and acetate/isothiocyanate bridges. Variable‐temperature (3‐300 K) magnetic studies have been performed and data analyses reveal that the dinuclear nickel(II) units show a weak ferromagnetic coupling in complex 1 (J=+ 3.70) and a weak antiferromagnetic coupling in complex 2 (J=‐ 0.87 cm– 1). The catalytic promiscuity of the complexes in terms of two different bio‐relevant catalytic activities like oxidation (catecholase) and hydroxylation (phosphatase) has been thoroughly explored. Role of an auxiliary electronegative atom present on the ligand backbone and binding approach of the substrate to the metal centres during the catalytic activities have been scrutinized by DFT calculation. Several experimental techniques have been utilised to evaluate the mechanistic interpretation of catecholase like activity. And finally, mechanistic pathway of both the bio activities are demonstrated.
Synthesis, Structure and Configuration of Streptanoate
Authors: Li Mei‐Mei , Xu Ze‐Jun , Wu Yikang , Liu Bo 
Published: February 2018

AbstractDiastereomers (of different relative configurations) of the reported gross structure and all its possible alternatives were synthesized. However, none of them showed NMR and optical rotation consistent with those in the literature. The very confusing data discrepancies and the unavoidable doubt about the identity of the natural sample caused by the deficiencies (e. g. inadequate sample purity, unsatisfactory quality of NMR spectra and human errors) hidden in the previous work were eventually resolved through reveal of a so far unnoticed effect of CDCl3 on the NMR of streptanoate in DMSO‐d6. The new systematic spectral data show that streptanoate should have a hydroxybutanoylamino acid gross structure with a (2R,2’R) absolute configuration.
A Spontaneous 3D Bone‐On‐a‐Chip for Bone Metastasis Study of Breast Cancer Cells
Authors: Hao Sijie , Ha Laura , Cheng Gong , Wan Yuan , Xia Yiqiu , Sosnoski Donna M. , Mastro Andrea M. , Zheng Si‐Yang 
Published: February 2018

AbstractBone metastasis occurs at ≈70% frequency in metastatic breast cancer. The mechanisms used by tumors to hijack the skeleton, promote bone metastases, and confer therapeutic resistance are poorly understood. This has led to the development of various bone models to investigate the interactions between cancer cells and host bone marrow cells and related physiological changes. However, it is challenging to perform bone studies due to the difficulty in periodic sampling. Herein, a bone‐on‐a‐chip (BC) is reported for spontaneous growth of a 3D, mineralized, collagenous bone tissue. Mature osteoblastic tissue of up to 85 µm thickness containing heavily mineralized collagen fibers naturally formed in 720 h without the aid of differentiation agents. Moreover, co‐culture of metastatic breast cancer cells is examined with osteoblastic tissues. The new bone‐on‐a‐chip design not only increases experimental throughput by miniaturization, but also maximizes the chances of cancer cell interaction with bone matrix of a concentrated surface area and facilitates easy, frequent observation. As a result, unique hallmarks of breast cancer bone colonization, previously confirmed only in vivo, are observed. The spontaneous 3D BC keeps the promise as a physiologically relevant model for the in vitro study of breast cancer bone metastasis.
Scalable 2D Mesoporous Silicon Nanosheets for High‐Performance Lithium‐Ion Battery Anode
Authors: Chen Song , Chen Zhuo , Xu Xingyan , Cao Chuanbao , Xia Min , Luo Yunjun 
Published: February 2018

AbstractConstructing unique mesoporous 2D Si nanostructures to shorten the lithium‐ion diffusion pathway, facilitate interfacial charge transfer, and enlarge the electrode–electrolyte interface offers exciting opportunities in future high‐performance lithium‐ion batteries. However, simultaneous realization of 2D and mesoporous structures for Si material is quite difficult due to its non‐van der Waals structure. Here, the coexistence of both mesoporous and 2D ultrathin nanosheets in the Si anodes and considerably high surface area (381.6 m2 g−1) are successfully achieved by a scalable and cost‐efficient method. After being encapsulated with the homogeneous carbon layer, the Si/C nanocomposite anodes achieve outstanding reversible capacity, high cycle stability, and excellent rate capability. In particular, the reversible capacity reaches 1072.2 mA h g−1 at 4 A g−1 even after 500 cycles. The obvious enhancements can be attributed to the synergistic effect between the unique 2D mesoporous nanostructure and carbon capsulation. Furthermore, full‐cell evaluations indicate that the unique Si/C nanostructures have a great potential in the next‐generation lithium‐ion battery. These findings not only greatly improve the electrochemical performances of Si anode, but also shine some light on designing the unique nanomaterials for various energy devices.
Intracerebroventricular administration of growth hormone induces morphological changes in pyramidal neurons of the hippocampus and prefrontal cortex in adult rats
Authors: Olivares‐Hernández Juan David , García‐García Fabio , Camacho‐Abrego Israel , Flores Gonzalo , Juárez‐Aguilar Enrique 
Published: February 2018

AbstractA growing body of evidence suggests that growth hormone (GH) affects synaptic plasticity at both the molecular and electrophysiological levels. However, unclear is whether plasticity that is stimulated by GH is associated with changes in neuron structure. This study investigated the effect of intracerebroventricular (ICV) administration of GH on the morphology of pyramidal neurons of the CA1 region of the dorsal hippocampus and layer III of the prefrontal cortex. Male Wistar rats received daily ICV injections of GH (120 ng) for 7 days, and they were euthanized 21 days later. Changes in neuronal morphology were evaluated using Golgi‐Cox staining and subsequent Sholl analysis. GH administration increased total dendritic length in the CA1 region of the dorsal hippocampus and prefrontal cortex. The Sholl analysis revealed an increase in dendritic length of the third to eighth branch orders in the hippocampus and from the third to sixth branch orders in the prefrontal cortex. Interestingly, GH treatment increased the density of dendritic spines in both brain regions, favoring the presence of mushroom‐like spines only in the CA1 hippocampal region. Our results indicated that GH induces changes in the length of dendritic trees and the density of dendritic spines in two high‐plasticity brain regions, suggesting that GH‐induced synaptic plasticity at the molecular and electrophysiological levels may be associated with these structural changes in neurons.
Phenotypic and genetic divergence among island populations of sika deer (Cervus nippon) in southern Japan: a test of the local adaptation hypothesis
Authors: Terada Chisato , Saitoh Takashi 
Published: February 2018

AbstractTransplant and common garden experiments have been used in studies on local adaptation, but are difficult to be conducted for large animals with long life span. A previous study on the southern Japanese islands demonstrated that relative limb lengths of sika deer (Cervus nippon) were short on islands with steep slopes. We hypothesized that this morphological variation was evidence for local adaptation, and tested this hypothesis by comparing phenotypic divergence with neutral genetic divergence among eight populations of the sika deer in the southern Japanese islands. Divergence patterns differed between the phenotypic and neutral genetic features. Genetic similarity was high among individuals on Kyushu (OI, KGS, and KGK). Individuals on Tanegashima (TN) and Yakushima (YK) also constituted a group, whereas individuals on Tsushima (TS), Wakamatsujima (WM), and Kuchinoerabujima (KE) formed a genetically distinct group. Phenotypic data indicated that individuals from TS, OI, KGS, and KGK exhibited similarity, whereas individuals on YK formed an isolated group that was separated from the other populations. The degree of phenotypic divergence was larger than that of neutral genetic divergence between TN and YK. These results suggest that divergent selection worked between two of the eight island populations (TN and YK). The morphological trait of captive‐bred individuals from TN and YK, which had never experienced their original environments, retained their original morphological features. By combining the results of multiple analyses, we found that the difference in relative limb length between the two populations was consistent with local adaptation hypothesis, although conclusive results were not obtained for the other populations.
Antibacterial applications of α-Fe2O3/Co3O4 nanocomposites and study of their structural, optical, magnetic and cytotoxic characteristics
Authors: Bhushan Mayank , Kumar Yogesh , Periyasamy Latha , Viswanath Annamraju Kasi 
Published: February 2018

Abstract
Owing to their multiple mechanisms of bactericidal activity, inorganic metal oxides and hybrid metal oxide nanocomposites may serve as a new class of effective disinfectants. Among metal oxide nanoparticles, iron oxide nanoparticles exhibit minimal or no cytotoxicity to human cells with very efficient bactericidal properties over a wide spectrum of bacteria. This paper presents the very first report on antibacterial properties of novel nanocomposites of iron oxide and cobalt oxide nanoparticles against pathogenic bacterial strains B. subtilis, S. aureus, E.coli and S. typhi. The enhanced bactericidal activity of the Fe/Co oxide nanocomposite was the result of synergistic effect of iron oxide and cobalt oxide nanoparticles. The nanocomposites were synthesized using co-precipitation route with increasing cobalt content in the sample and further characterized using XRD, TEM, Raman and VSM to investigate structural, optical and magnetic properties of the prepared nanocomposites, respectively. Also, the prepared nanocomposites were highly biocompatible and found non-toxic to human cell line MCF7.
Tissue sterol composition in Atlantic salmon (Salmo salarL.) depends on the dietary cholesterol content and on the dietary phytosterol:cholesterol ratio, but not on the dietary phytosterol content
Authors: Sissener Nini H. , Rosenlund Grethe , Stubhaug Ingunn , Liland Nina S. 
Published: February 2018

AbstractThe aim of the study was to investigate how the dietary sterol composition, including cholesterol, phytosterol:cholesterol ratio and phytosterols, affect the absorption, biliary excretion, retention, tissue storage and distribution of cholesterol and individual phytosterols in Atlantic salmon (Salmo salarL.). A feeding trial was conducted at two different temperatures (6 and 12°C), using nine different diets with varying contents of phytosterols, cholesterol and phytosterol:cholesterol ratio. Cholesterol retention values were clearly dependent on dietary cholesterol, and showed that fish fed cholesterol levels <1000 mg/kg feed produced considerable quantities of cholesterolde novo. Despite this production, cholesterol content increased with increasing dietary cholesterol in liver, plasma, bile, muscle, adipose tissue and whole fish at 12°C, and in plasma, bile and whole fish at 6°C. The tissue sterol composition generally depended on the dietary cholesterol content and on the dietary phytosterol:cholesterol ratio, but not on the dietary phytosterol content in itself. Campesterol and brassicasterol appeared to be the phytosterols with the highest intestinal absorption in Atlantic salmon. There was a high biliary excretion of campesterol, but not of brassicasterol, which accumulated in tissues and particularly in adipose tissue, with 2-fold-higher retention at 12°C compared with 6°C. Campesterol had the second highest retention of the phytosterols in the fish, but with no difference between the two temperatures. Other phytosterols had very low retention. Although brassicasterol retention decreased with increasing dietary phytosterols, campesterol retention decreased with increasing dietary cholesterol, indicating differences in the uptake mechanisms for these two sterols.
Fostering adoption of conservation technologies: a case study with wildlife law enforcement rangers
Authors: Sintov Nicole , Seyranian Viviane , Lyet Arnaud 
Published: February 2018

AbstractNew technologies can aid the success of conservation outcomes. Technology alone will not however guarantee conservation success; this hinges on user adoption. Hence, there is a need to understand users’ adoption decisions and how to account for these to streamline the introduction of new technologies. Wildlife law enforcement rangers constitute a key end-user group for conservation technologies, and although some studies have focused on ranger experiences, and on the impacts of policing technologies on crime rates, few have addressed technology adoption among law enforcement personnel, and none among rangers. To address this gap we conducted a case study focused on a new technology called the Protection Assistant for Wildlife Security (PAWS), which was developed to deter poaching by improving the deployment of wildlife law enforcement ranger foot patrols. We evaluated the impacts of an educational programme on the willingness of Indonesia-based rangers to adopt the tool. Following the programme, rangers reported high levels of willingness to adopt PAWS. Furthermore, the more engaged rangers were in the programme, the more useful and easy to use they perceived PAWS to be, and the stronger their adoption intentions. In contrast, rangers who were more resistant to technology from the outset were less engaged in the programme, highlighting the importance of identifying and addressing sources of resistance. Overall, the findings of this case study stress the significance of accounting for and educating end users in disseminating conservation technologies, reinforcing the importance of accounting for human dimensions of conservation.
Intrauterine growth restriction combined with a maternal high-fat diet increased adiposity and serum corticosterone levels in adult rat offspring
Authors: Zinkhan E. K. , Yu B. , Callaway C. W. , McKnight R. A. 
Published: February 2018

AbstractIntrauterine growth restriction (IUGR) and fetal exposure to a maternal high-fat diet (HFD) independently increase the risk of developing obesity in adulthood. Excess glucocorticoids increase obesity. We hypothesized that surgically induced IUGR combined with an HFD would increase adiposity and glucocorticoids more than in non-IUGR offspring combined with the same HFD, findings that would persist despite weaning to a regular diet. Non-IUGR (N) and IUGR (I) rat offspring from dams fed either regular rat chow (R) or an HFD (H) were weaned to either a regular rat chow or an HFD. For non-IUGR and IUGR rats, this study design resulted in three diet groups: offspring from dams fed a regular diet and weaned to a regular diet (NRR and IRR), offspring rats from dams fed an HFD and weaned to a regular diet (NHR and IHR) and offspring from dams fed an HFD and weaned to an HFD (NHH and IHH). Magnetic resonance imaging or fasting visceral and subcutaneous adipose tissue collection occurred at postnatal day 60. IHH male rats had greater adiposity than NHH males, findings that were only partly normalized by weaning to a regular chow. IHH male rats had a 10-fold increase in serum corticosterone levels. IHH female rats had increased adiposity and serum triglycerides. We conclude that IUGR combined with an HFD throughout life increased adiposity, glucocorticoids and triglycerides in a sex-specific manner. Our data suggest that one mechanism through which the perinatal environment programs increased adiposity in IHH male rats may be via increased systemic glucocorticoids.