bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Benefits of Photodynamic Therapy Using Talaporfin Sodium in Patients With Isocitrate Dehydrogenase-Wildtype Diagnosed Glioblastoma: A Retrospective Study of 100 Cases
Authors: Fujimoto Yosuke , Fujita Yuichi , Tanaka Kazuhiro , Nagashima Hiroaki , Yamanishi Shunsuke , Ikeuchi Yusuke , Iwahashi Hirofumi , Sanada Shoji , Muragaki Yoshihiro , Sasayama Takashi 
Published: November 2024

BACKGROUND AND OBJECTIVES:
Photodynamic therapy (PDT) with talaporfin sodium is an intraoperative local therapy administered after the surgical removal of malignant gliomas. However, its clinical efficacy in a large patient population has not been determined. To analyze the clinical outcomes and prognosis in isocitrate dehydrogenase (IDH)-wildtype glioblastoma patients treated with PDT.

METHODS:
This retrospective study included patients with newly diagnosed IDH-wildtype glioblastoma treated at Kobe University Hospital between January 2013 and December 2022. PDT involves irradiation of the resection cavity with a 664-nm semiconductor laser after an intravenous infusion of talaporfin sodium. The main outcome measures were the recurrence patterns and survival times, which were compared between the PDT and non-PDT groups. Univariate and multivariate analyses were used to determine the prognostic factors. In addition, adverse events and prognostic factors in the PDT group were analyzed.

RESULTS:
A total of 44 and 56 patients were included in the PDT and non-PDT groups, respectively. The local recurrence rate was significantly lower in the PDT group than in the non-PDT group (51.3% vs 83.9%), whereas the distant recurrence and dissemination rates were significantly higher in the PDT group than in the non-PDT group (48.7% vs 16.1%). Two grade 3 adverse events were observed in the PDT group. The median progression-free survival and overall survival times were significantly longer in the PDT group than in the non-PDT group (progression-free survival: 10.8 vs 9.3 months, respectively, and overall survival: 24.6 vs 17.6 months, respectively). Multivariate analysis of the PDT groups revealed that younger age was an independent prognostic factor.

CONCLUSION:
PDT with talaporfin sodium provided effective local control with minimal adverse effects. The survival time of the patients treated with PDT was significantly longer than that of the patients who did not receive PDT. Therefore, a randomized controlled clinical trial on PDT is warranted.
A genome-engineered tool set for Drosophila TGF-β/BMP signaling studies
Authors: Ell Clara-Maria , Safyan Abu , Chayengia Mrinal , Kustermann Manuela M. M. , Lorenz Jennifer , Schächtle Melanie , Pyrowolakis George 
Published: November 2024

ABSTRACT
Ligands of the TGF-β/BMP superfamily are crucially involved in the regulation of growth, patterning and organogenesis and can act as long-range morphogens. Essential for understanding TGF-β/BMP signaling dynamics and regulation are tools that allow monitoring and manipulating pathway components at physiological expression levels and endogenous spatiotemporal patterns. We used genome engineering to generate a comprehensive library of endogenously epitope- or fluorescent-tagged versions of receptors, co-receptors, transcription factors and key feedback regulators of the Drosophila BMP and Activin signaling pathways. We demonstrate that the generated alleles are biologically active and can be used for assessing tissue and subcellular distribution of the corresponding proteins. Furthermore, we show that the genomic platforms can be used for in locus structure-function and cis-regulatory analyses. Finally, we present a complementary set of protein binder-based tools, which allow visualization as well as manipulation of the stability and subcellular localization of epitope-tagged proteins, providing new tools for the analysis of BMP signaling and beyond.
Retinal ganglion cell-derived semaphorin 6A segregates starburst amacrine cell dendritic scaffolds to organize the mouse inner retina
Authors: James Rebecca E. , Hamilton Natalie R. , Huffman Lola Nicole , Brown Matthew P. , Neckles Victoria N. , Pasterkamp R. Jeroen , Goff Loyal A. , Kolodkin Alex L. 
Published: November 2024

ABSTRACT
To form functional circuits, neurons must settle in their appropriate cellular locations, and then project and elaborate neurites to contact their target synaptic neuropils. Laminar organization within the vertebrate retinal inner plexiform layer (IPL) facilitates pre- and postsynaptic neurite targeting, yet the precise mechanisms underlying establishment of functional IPL subdomains are not well understood. Here, we explore mechanisms defining the compartmentalization of OFF and ON neurites generally, and OFF and ON direction-selective neurites specifically, within the developing mouse IPL. We show that semaphorin 6A (Sema6A), a repulsive axon guidance cue, is required for delineation of OFF versus ON circuits within the IPL: in the Sema6a null IPL, the boundary between OFF and ON domains is blurred. Furthermore, Sema6A expressed by retinal ganglion cells (RGCs) directs laminar segregation of OFF and ON starburst amacrine cell dendritic scaffolds, which themselves serve as a substrate upon which other retinal neurites elaborate. These results demonstrate that RGCs, the first type of neuron born within the retina, play an active role in functional specialization of the IPL.
The role of mesenchymal cells in cholangiocarcinoma
Authors: Sueca-Comes Mireia , Rusu Elena Cristina , Ashworth Jennifer C. , Collier Pamela , Probert Catherine , Ritchie Alison , Meakin Marian , Mongan Nigel P. , Egbuniwe Isioma U. , Andersen Jesper Bøje , Bates David O. , Grabowska Anna M. 
Published: November 2024

ABSTRACT
The tumour microenvironment (TME) significantly influences tumour formation and progression through dynamic interactions. Cholangiocarcinoma (CCA), a highly desmoplastic tumour, lacks early diagnostic biomarkers and has limited effective treatments owing to incomplete understanding of its molecular pathogenesis. Investigating the role of the TME in CCA progression could lead to better therapies. RNA sequencing was performed on seven CCA patient-derived xenografts (PDXs) and their corresponding patient samples. Differential expression analysis was conducted, and Qiagen Ingenuity Pathway Analysis was used to predict dysregulated pathways and upstream regulators. PDX- and cell line-derived spheroids, with and without immortalised mesenchymal stem cells, were grown and analysed for morphology, growth and viability. Histological analysis confirmed biliary phenotypes. RNA sequencing indicated upregulation of extracellular matrix-receptor interaction and PI3K-AKT pathways in the presence of mesenchymal cells, with several genes linked to poor survival. Mesenchymal cells restored the activity of inhibited cancer-associated kinases. Thus, adding mesenchymal cells to CCA spheroid models restored key paracrine signalling pathways lost in PDXs, enhancing tumour growth and viability. These findings highlight the importance of including stromal components in cancer models to improve pre-clinical studies.
Spiski etnoperformans (cz. 2)
Authors: Barański Janusz 
Published: November 2024

Przedmiotem artykułu są dwie imprezy regionalne, konkurs i przegląd spiskiego folkloru: Spiskie Zwyki i Spiska Watra. Organizowane są każdego roku – odpowiednio – zimą i latem na Zamku Dunajec w Niedzicy i w Gminnym Ośrodku Kultury w Łapszach Niżnych z siedzibą w Niedzicy. Dyskutowany region jest niewielkim fragmentemkrainy historycznej, której główna część znajduje się na terytorium Słowacji. Stanowił przez setki lat część Królestwa Węgier, stąd jego specyfika i odrębność kulturowa. W świetle przeprowadzonych badań etnograficznych analizowane imprezy należą do praktyk typuetnoperformansu, który łączy aspekt artystyczny ze społecznym. Pierwszy jest wyrazem regionalnej estetyki i artyzmu (folklor słowno-muzyczny, taniec, strój regionalny), drugi – rytualnego charakteru imprez służących procesom tożsamościowym. W świetle poczynionych ustaleń omawiane przypadki etnoperformansu reprezentują spuściznę kulturową w trybie dziedzictwa. W tym rozumieniu są formą odmienną od dawniejszej tradycji i można je uznać za rodzaj metakulturowej praktyki oraz metakulturowego komentarza wobec kultury regionalnej.
Supervisão de enfermagem hospitalar: do ingresso na função ao planejamento organizacional
Authors: Santos Yago Hernandez Ferreira dos , Bolina Alisson Fernandes , Bueno Alexandre de Assis , Paranaguá Thatianny Tanferri de Brito , Souza Maressa Aguiar de , Aguiar Shena Carolina da Silva 
Published: November 2024

Objetivo: analisar a supervisão de enfermagem hospitalar e suas implicações para o planejamento organizacional, na perspectiva de enfermeiros supervisores. Método: estudo qualitativo-exploratório, conduzido com 13 supervisores de enfermagem de um hospital de ensino na Região Centro-oeste do Brasil, no ano de 2020. Realizada análise temática indutiva. Resultados: foram identificados três temas: Ingresso na função de supervisão, Competências para a supervisão e Planejamento na função de supervisão. Observou-se perspectiva normativa do planejamento, com enfoque em atividades burocráticas e ausência de participação dos demais integrantes da equipe. Os dados revelaram limitações relacionadas à descrição de um referencial para sistematizar a prática do planejamento. Conclusão: na percepção dos enfermeiros supervisores, os incentivos e o fomento à capacitação são fundamentais para o ingresso na função, o que permite e exige o desenvolvimento de competências. O processo é pouco democrático, carece de capacitação e, por conseguinte, reflete na capacidade responsiva do planejamento da unidade.
Spatiotemporal distribution and meteorological factors of hemorrhagic fever with renal syndrome in Hubei province
Authors: Li Hang , Yang Rui , Guan Xuhua , Huang Xiaobo , Jiang Honglin , Tan Liangfei , Xiong Jinfeng , Peng Mingjun , Zhang Tianbao , Yao Xuan 
Published: November 2024

Background
Hemorrhagic fever with renal syndrome (HFRS) is a vital rodent-borne disease, and poses a serious public health threat in Hubei province. We aimed to explore the spatiotemporal distribution of HFRS in Hubei province during 2005–2022, and the effects of meteorological factors.

Methods
Data on HFRS cases at the county level in Hubei province during 2005–2022 were obtained from the Chinese Center for Disease Prevention and Control Information System. The monthly meteorological data at the city level was extracted from the China Meteorological Data Sharing Service System from 2016 to 2020. Descriptive analyses, joinpoint regression model, spatial correlation analyses, Geodetector model and autoregressive integrated moving average (ARIMA) model were conducted to investigate the epidemic characteristics, temporal trend, spatial distribution, influencing factors of HFRS and predict its trend.

Results
A total of 6,295 cases were reported in Hubei province during 2005–2022, with an average incidence of 6/1,000,000. Most cases were males (74.52%) and aged 40–69 years (71.87%). The monthly HFRS cases showed two seasonal peaks, which were summer (May to June) and winter (November to December). The HFRS incidence remained fluctuating at a low level during 2005–2015, followed an increasing trend during 2015–2018, and then decreased during 2018–2022. Hotspots were concentrated in the center of Hubei province in all 3 periods, including Qianjiang, Tianmen and some counties from Xiangyang, Jingmen and Jingzhou cities. The distribution of HFRS had a positive association with wind speed, while a “V”-shaped correlation with mean temperature, with an explanatory power of 3.21% and 1.03% respectively (both P <0.05). The ARIMA model predicted about 1,223 cases occurred in the next 3 years.

Conclusions
HFRS cases showed seasonal fluctuation and spatial clustering in Hubei province. Central plain areas showed high risk of HFRS. Wind speed and mean temperature had significant effects on the transmission of HFRS in Hubei province. The results alert health authorities to conduct disease-climate surveillance and comprehensive prevention strategies, especially in high-risk counties.
Bridging the “know-do” gap to improve active case finding for tuberculosis in India: A qualitative exploration into national tuberculosis elimination program staffs’ perspectives
Authors: Shewade Hemant Deepak , Ravichandran Prabhadevi , Pradeep S. Kiran , Kiruthika G. , Shanmugasundaram Devika , Chadwick Joshua , Iyer Swati , Chowdhury Aniket , Tumu Dheeraj , Shah Amar N. , Vadera Bhavin , Roddawar Venkatesh , Mattoo Sanjay K. , Rade Kiran , Rao Raghuram , Murhekar Manoj V. 
Published: November 2024

Background
In 2022, India’s national tuberculosis (TB) elimination program (NTEP) commissioned a national level evaluation of active case finding (ACF) for TB to guide evidence-based strategic planning. As part of this evaluation, based on secondary data analysis we observed that the quality of ACF was suboptimal in 2021. Hence, this study aimed to understand the enablers, barriers, and suggested solutions to improve ACF for TB in India from NTEP staff (provider) perspective.

Methods
This was a descriptive qualitative study involving key informant interviews from six districts and eight states, conducted between February and August 2023. We purposively selected key state- district- and sub-district-level program managers and implementers who were experienced and vocal. The interviews were audio recorded and transcribed verbatim by research interns and investigators. Two investigators independently did manual descriptive thematic analysis, and a third investigator resolved inconsistencies. The themes and categories emerged by collating together the results of the coding process.

Results
A total of 34 key informant interviews were conducted and of these, four were repeat interviews. Adequate budgets for ACF including incentives, performance review mechanism, engagement of all stakeholders, adopting a community friendly approach, use of rapid diagnostic tests and digitalization were the perceived enablers. In some states ACF was implemented in general population (not restricted to high-risk population) following directives at state level. There were limited mechanisms to ensure ACF quality indicators were met before disbursing incentives and cross-verification of the aggregate ACF care cascade numbers that were reported in Ni-kshay (electronic TB information management system under NTEP). In addition to the state and district level implementers having limited understanding of concepts around ACF (quality indicators, number needed to screen and yield), we also inferred the presence of a ‘know-do’ gap for many activities under ACF. The suggested solutions were around capacity building and quality improvement strategies.

Conclusion
The existing national ACF guidance should be revised to emphasize capacity building, need to carry out ACF in high-risk (not general) population, quality control-linked incentives, and regular implementation monitoring of the activities. This should contribute towards better coverage and improved quality translating into better ACF outcomes.
Myroides species, pathogenic spectrum and clinical microbiology sight in Mexican isolates
Authors: Colín-Castro Claudia Adriana , Ortiz-Álvarez Jossue Mizael , Hernández-Pérez Cindy Fabiola , Hernández-Durán Melissa , García-Hernández María de Lourdes , Martínez-Zavaleta María Guadalupe , Becerra-Lobato Noé , Cervantes-Hernández Mercedes Isabel , Rosas-Alquicira Graciela , Cerón-González Guillermo , Méndez-Sotelo Braulio Josué , García-Contreras Rodolfo , Franco-Cendejas Rafael , López-Jácome Luis Esaú 
Published: November 2024

Introduction
Myroides is a bacterial genus of opportunistic bacteria responsible for diverse infections including in the skin and soft tissues, urinary tract, cardiovascular system, and bacteremia, although the incidence of its reported infections is low, it is increasing, likely due the use of better bacterial identification methods, but also perhaps due an increase in its prevalence. In addition, their pathogenic role is limited in terms of reporting their microbial physiology, so the present work provides information in this regard in addition to the information that is available in the international literature.

Objective
To describe the microbiological and genetic characteristics of seven different Myroides spp. clinical strains and comment on their phylum, pathogenic and resistance characteristics.

Methods
Seven Myroides spp., strains associated with infections were included from 1/January/2012 to 1/January/20 and identified by miniaturized biochemistry and MALDI-ToF. Susceptibility tests were performed according to CLSI recommendations by broth microdilution. Whole genome sequencing was performed for each strain and bioinformatics analysis were performed.

Results
Strains were identified at genus level by two methodologies. Our results revealed that likely four strains belong to the species Myroides odoratimimus, while the other two may be undescribed ones. Remarkably, all isolates harbored several genes encoding antibiotic resistance determinants for ß-lactams, aminoglycosides and glycopeptides and in concordance, presented high levels of resistance, against these antibiotics (AK and GN both 100%, ATM, CAZ and FEP 100%, e.g.); moreover, the presences of carbapenemases were evidenced by meropenem (mCIM) and imipenem (CARBA NP) degrading activity in six isolates and two strains possessed plasmids harboring mainly ribosomal RNA genes, tRNAs and genes encoding proteins with unknown functions.

Conclusions
Our study increases the knowledge about the biology of this understudied genus and highlights the potential of Myroides to emerge as a broader cause of recalcitrant opportunistic infections.
Quality of life, physical symptoms, and psychological symptoms according to the status of chronic vestibulopathy
Authors: Han Sang-Yoon , Lee Sang-Yeon , Suh Myung-Whan , Lee Jun Ho , Park Moo Kyun 
Published: November 2024

Objectives
Symptomatic vestibulopathy impairs patients’ lives. However, few studies have explored the lives of patients with compensated or asymptomatic vestibulopathy. This study investigated the quality of life (QOL), psychological health, and physical function of patients with vestibulopathy.

Materials and methods
Using the eighth Korea National Health and Nutrition Examination Survey database, we included individuals with data on demographic factors, diabetes, hypertension, dizziness experiences, pure-tone audiometry, video head impulse test (vHIT), Health-related Quality of Life Instrument with 8 Items, General Anxiety Disorder 7-item scale, stress, and walking and sitting times. Participants were classified into the following groups: an uncompensated group with abnormal vHIT result and chronic dizziness, a compensated group with abnormal vHIT result and a history of dizziness, an asymptomatic group with abnormal vHIT result and no history of dizziness, and a normal group without abnormal vHIT result or a history of dizziness.

Results
Uncompensated vestibulopathy was more common in older individuals and women. The uncompensated group showed impairments in climbing stairs (P < 0.001), pain (P < 0.001), vitality (P = 0.001), working (P < 0.001), depression (P < 0.001), sleep (P = 0.001), happiness (P = 0.002), anxiety (P = 0.006), and stress (P = 0.003). The compensated group showed deficits in pain (P < 0.001), work (P = 0.006), sleep (P = 0.001), and happiness (P = 0.001). The asymptomatic group had no deficits in QOL, psychological health, or physical function. These tendencies were similar after controlling for age and gender.

Conclusion
Vestibulopathy with a history of dizziness has a long-lasting impact on QOL and emotional status, even after compensation. Uncompensated vestibulopathy has a significant effect on QOL and mental health. Notably, though, the compensated group also showed a reduction in QOL. Appropriate interventions for each category of patients should be provided based on their impaired functions.
Genetic background of high myopia in children
Authors: Šenk Urh , Čižman Bernard , Writzl Karin , Tekavčič Pompe Manca 
Published: November 2024

Objective
High myopia is a significant risk factor for irreversible vision loss and can occur in isolation or as a component of various syndromes. However, the genetic basis of early-onset high myopia remains poorly understood. We aimed to identify the causative genetic variants for high myopia in a cohort of Slovenian children.

Methods
The study included children referred to a tertiary paediatric ophthalmology centre at the University Eye Clinic in Ljubljana between 2010 and 2022. The participants met the following inclusion criteria: age ≤ 15 years and high myopia ≤-5.0 D before the age of 10 years. Genetic analysis included exome sequencing and/or molecular karyotyping. Participants were categorized based on clinical presentation: high myopia with systemic involvement, high myopia with ocular involvement, and isolated high myopia.

Results
Genetic analysis of 36 probands revealed a genetic cause of high myopia in 22 (61.1%) children. Among those with systemic involvement (50.0%), genetic causes were identified in 13 out of 18 children, with Stickler’s and Pitt-Hopkins being the most common syndromes. Among cases of high myopia with ocular involvement (38.9%), a genetic cause was found in 8 out of 14 probands, including (likely) pathogenic variants in genes related to retinal dystrophies (CACNA1F, RPGR, RP2, NDP). The non-syndromic ARR3- associated high myopia was identified in the isolated high myopia group.

Conclusions
A genetic cause of high myopia was identified in 61.1% of children tested, demonstrating the value of genetic testing in this population for diagnosis and proactive counseling.
Educator’s role in preventing ageism
Authors: Mihevc Špela , Grušovnik Mušič Anja , Rotar Pavlič Danica 
Published: November 2024

Aims
This study aims to contribute to a better understanding of the educator’s role in imparting knowledge to healthcare students regarding ageism in Slovenia.

Methods
Educators in Slovenian secondary nursing schools and in medical and health science faculties were asked to evaluate their practical experience in working with older people, their knowledge of gerontology and working with older people, and their attitude toward working with older people using an online questionnaire. They were also asked to rate their opinion about ageism on a Likert scale, along with reasons for ageism, skills that would help reduce ageism, their opinion about trainees’ attitudes toward older people, and their assessment of certain facts about planning training. The reliability of the questionnaire was tested using Cronbach’s alpha. The Kruskal‒Wallis test, Mann‒Whitney U test, and independent samples t-test were used to determine differences between the groups.

Results
The participants fully agree that the knowledge imparted could contribute to reducing age discrimination among healthcare students. Those that responded that their gerontological knowledge is good had significantly higher ranked responses regarding the reasons for ageism. On average, they agreed with the statements about planning their teaching activities and facilitating the acquisition of knowledge.

Conclusions
Ageism is a challenge for modern society that requires a comprehensive approach to prevent and combat this form of discrimination. Awareness-raising, education, and policy change can create a fairer and more respectful society for all generations. Educators are insufficiently aware of their role in preventing ageism. Practitioners recognize it when they work directly with students. There are opportunities to update curricula and teaching methods.