bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengembangan Protokol Isolasi DNA Genom Tanaman Durian Dengan Menggunakan Modifikasi Bufer CTAB
Authors: Sundari Sundari 
Published: January 2019

AbstrakProtokol dan metode sederhana, efisien untuk isolasi DNA genom tanaman durian yang banyak mengandung phenol dan residu polisakarida telah dihasilkan. Pada penelitian ini, digunakan protokol isolasi DNA tumbuhan dengan metode CTAB yang dimodifikasi sebagai protokol yang efisien untuk membuang polisakarida, phenol dan lendir yang sangat melimpah pada tanaman durian. Obyek penelitian ini terdiri dari protocol CTAB yang dimodifikasi tahap inkubasi dan presipitasi pemurnian DNA genom dari phenol dan polisakarida. Perbandingan 2 protokol isolasi DNA durian dengan CTAB standard an CTAB modifikasi menunjukkan bahwa metode CTAB modifikasi menghasilkan whole genom durian cukup murni rata rata 1,99 dan berhasil diamplifikasi dengan PCR-RAPD.Kata kunci: isolasi, DNA, polisakarida, CTAB, modifikasi, . AbstractThe simple and efficient method for genomic DNA isolation protochol from durian , its woody fruit crops containing high polysaccharide levels has been described here. In the present study, using modified CTAB for plant DNA isolation protocols were studied for removing the highly concentrated polysaccharides from genomic DNA of woody fruit crops.This method involves the modified CTAB at the incubate and precipitate procedure employing DNA purification step to remove polysaccharides and phenol residu. Compared with the two studied DNA isolation protocols of durian using standart CTAB and modified CTAB the everage yield high quality DNA whole genom is 1,99 purity and DNA was suitable for PCR and RAPD analyses.Keyword: isolation, DNA, polysaccharides, phenol residu, CTAB
Sampah Rumah Tangga di Ternate
Authors: Bahtiar Bahtiar , Ahmad Zulkifli , Pobi Wiyana 
Published: January 2019

Abstrak Jumlah penduduk Kota Ternate 212.997 jiwa, luas wilayah 111,39 km2 dengan kepadatan 1.865,42 jiwa/ km2. Apabila kita menggunaan pendekatan paradigma “sampah sebagai barang berguna” maka jumlah penduduk sebanyak itu menghasilkan volume sampah yang berpotensi untuk dimanfaatkan dan diolah kembali. Terkait kepentingan pengelolaan dan pemanfaatan sampah di Kota Ternate ke depan, maka kedudukan sampah merupakan sebuah potensi. Deskripsi potensi sampah harus diawali dengan pengenalan komposisi dan timbulan sampah yang ada, terutama sampah rumah tangga. Gambaran komposisi akan memberikan informasi tentang komponen yang terdapat dalam buangan padat beserta distribusinya. Timbulan sampah memberikan informasi banyaknya sampah rumah tangga yang dihasilkan. Data timbulan sampah juga diperlukan untuk desain sistem pengelolaan persampahan, seleksi jenis peralatan untuk transportasi sampah dan desain tempat pembuangan sampah. Kata Kunci:, komposisi sampah, rumah tangga, Kota Ternate Abstract The population of Ternate City is 212,997 people, the area of 111.39 km2 with the density of 1,865.42 people/km2. If we use the paradigm approach of "waste as a useful item" then the amount of population as much as it produces the volume of waste that has the potential to be utilized and reprocessed. Related to the management and utilization of waste in Ternate City in the future, the position of waste is a potential. Description of potential waste should be started with the introduction of composition and waste generation, especially household waste. The composition description will provide information on the components contained in solid waste and its distribution. Waste generation provides information on the amount of household waste generated. Waste generation data is also required for the design of waste management systems, the selection of equipment types for waste transport and the design of landfills. Keywords: composition of waste, household, Ternate city
IDENTIFIKASI STRUKTUR SENYAWA ANTIJAMUR DARI RUMPUT LAUT
Authors: Rahmawati Rahmawati 
Published: January 2019

Screening of plants antifungal compounds from Algae has been resulted. Screening was preceded by extraction of its fresh substance with benzene, methanol, and chloroform solvents and followed examination towards fungi Fusarium oxysforum, Sclerotium rolfsii, Aspergillus flavus, and Pythium spp. The antifungal effects were tested by well methode in potato dextrosa agar (PDA) medium. Analysis compound of benzene extract with preparative TLC methode (n-hexene : ethyl acetate : methanol = 7 : 2 : 1) obtained 5 compounds. Antifungal activity test of the fractions was done by the disk diffution methode with PDA medium. Identification of active compounds with spectroscophy method (IR and NMR) indicated the benzene extract analogue with fungicide Metalaxyl with the benzene ring and substituens of ester, alkyl, and amide. Identification of fractions showed that the compounds analogue with fungicides belong to carboxamide with the main substituen of benzene ring and ester/aldehids formed, alkyl, and amide.
MRI and Dose Selection in a Phase II Trial of Baricitinib with Conventional Synthetic Disease-modifying Antirheumatic Drugs in Rheumatoid Arthritis
Authors: Peterfy Charles , DiCarlo Julie , Emery Paul , Genovese Mark C. , Keystone Edward C. , Taylor Peter C. , Schlichting Doug E. , Beattie Scott D. , Luchi Monica , Macias William 
Published: January 2019

Objective.Magnetic resonance imaging (MRI) was used in a phase IIb study of baricitinib in patients with RA to support dose selection for the phase III program.Methods.Three hundred one patients with active RA who were taking stable methotrexate were randomized 2:1:1:1:1 to placebo or once-daily baricitinib (1, 2, 4, or 8 mg) for up to 24 weeks. One hundred fifty-four patients with definitive radiographic erosion had MRI of the hand/wrist at baseline and at weeks 12 and 24. Two expert radiologists, blinded to treatment and visit order, scored images for synovitis, osteitis, bone erosion, and cartilage loss. Combined inflammation (osteitis + 3× synovitis score) and total joint damage (erosion + 2.5× cartilage loss score) scores were calculated. Treatment groups were compared using ANCOVA adjusting for baseline scores.Results.Mean changes from baseline to Week 12 for synovitis were −0.10, −1.50, and −1.60 for patients treated with placebo, baricitinib 4 mg, and baricitinib 8 mg, respectively (p = 0.003 vs placebo for baricitinib 4 and 8 mg). Mean changes for osteitis were 0.00, −3.20, and −2.10 (p = 0.001 vs placebo for baricitinib 4 mg and p = 0.037 for 8 mg), respectively. Mean changes for bone erosion were 0.90, 0.10, and 0.40 (p = 0.089 for 4 mg and p = 0.275 for 8 mg), respectively, in these treatment groups.Conclusion.MRI findings in this subgroup of patients suggest suppression of synovitis, osteitis, and combined inflammation by baricitinib 4 and 8 mg. This corroborates previously demonstrated clinical efficacy of baricitinib and increases confidence that baricitinib 4 mg could reduce the radiographic progression in phase III studies. [Clinical trial registration number (www.ClinicalTrials.gov):NCT01185353]
Predictors of Effectiveness of Anakinra in Systemic Juvenile Idiopathic Arthritis
Authors: Saccomanno Benedetta , Tibaldi Jessica , Minoia Francesca , Bagnasco Francesca , Pistorio Angela , Guariento Andressa , Caorsi Roberta , Consolaro Alessandro , Gattorno Marco , Ravelli Angelo 
Published: January 2019

Objective.To seek predictors of therapeutic response to the interleukin (IL)-1 inhibitor anakinra in children with systemic-onset juvenile idiopathic arthritis (sJIA).Methods.The clinical charts of all patients with sJIA who were newly treated with anakinra at our center between 2004 and 2017 were reviewed retrospectively. Predictors included baseline demographic, clinical, and laboratory variables as well as previous or concomitant therapies. The effectiveness of anakinra was assessed at 1 year after treatment start. Complete clinical response (CCR) was defined as absence of fever, physician’s global assessment ≤ 1, count of active joints ≤ 1, negative C-reactive protein, and ≥ 75% reduction of corticosteroid dose. According to the intention-to-treat principle, patients who had anakinra discontinued before 1 year for any reasons other than disease remission were classified as nonresponders. Statistics included univariate and multivariable analyses.Results.Of the 62 patients included in the study, 24 (39%) met the criteria for CCR at 1 year, whereas 38 (61%) did not. On multivariable analysis, independent correlations with achievement of CCR were identified for shorter disease duration, lower active joint count, higher ferritin level, and greater activity of systemic manifestations. The area under the curve of the model was 0.83.Conclusion.Our findings help to delineate the clinical profile of patients with sJIA who are more likely to benefit from IL-1 blockade. They also underscore the need for studies aimed at examining the therapeutic role of early IL-1 inhibition and to identify biomarkers predicting response to either IL-1 or IL-6 antagonists.
Association of Smoking and Obesity on the Risk of Developing Primary Sjögren Syndrome: A Population-based Cohort Study
Authors: Servioli Luisa , Maciel Gabriel , Nannini Carlotta , Crowson Cynthia S. , Matteson Eric L. , Cornec Divi , Berti Alvise 
Published: January 2019

Objective.To explore the role of smoking and obesity in primary Sjögren syndrome (pSS).Methods.Olmsted County (Minnesota, USA) residents (n = 106) diagnosed with pSS from 2000 to 2015 were compared to 3 controls without pSS and matched for age and sex who were randomly selected from Olmsted County residents.Results.Current smokers were less likely to be pSS cases (OR 0.34, 95% CI 0.14–0.85), while there was no association between former smoking and case/control status (OR 1.27, 95% CI 0.80–2.03) compared to never smokers. Smoking status was not associated with antinuclear antibody, anti-SSA, anti-SSB, or rheumatoid factor positivity (p > 0.05). OR for obesity was 0.79 (95% CI 0.48–1.30).Conclusion.In this population-based study, current smoking was inversely associated with case/control status, while body mass index lacked any association.
Comorbidities in Patients with Rheumatoid Arthritis and Their Association with Patient-reported Outcomes: Results of Claims Data Linked to Questionnaire Survey
Authors: Luque Ramos Andres , Redeker Imke , Hoffmann Falk , Callhoff Johanna , Zink Angela , Albrecht Katinka 
Published: January 2019

Objective.To investigate the prevalence of comorbidities in a population-based cohort of persons with rheumatoid arthritis (RA) compared to matched controls and to examine their association with patient-reported outcomes in a survey sample.Methods.Data of 96,921 persons with RA [International Classification of Diseases, 10th ed (ICD-10) M05/M06] and 484,605 age- and sex-matched controls without RA of a German statutory health fund were studied regarding 26 selected comorbidities (ICD-10). A self-reported questionnaire, comprising joint counts [(tender joint count (TJC), swollen joint count (SJC)], functional status (Hannover Functional Ability Questionnaire), effect of the disease (Rheumatoid Arthritis Impact of Disease), and well-being (World Health Organization 5-item Well-Being Index; WHO-5) was sent to a random sample of 6193 persons with RA, of whom 3184 responded. For respondents who confirmed their RA (n = 2535), associations between comorbidities and patient-reported outcomes were analyzed by multivariable linear regression.Results.Compared to controls, all investigated comorbidities were more frequent in persons with RA (mean age 63 yrs, 80% female). In addition to cardiovascular risk factors, the most common were osteoarthritis (44% vs 21%), depression (32% vs 20%), and osteoporosis (26% vs 9%). Among the survey respondents, 87% of those with 0–1 comorbidity but only 77% of those with ≥ 8 comorbidities were treated by rheumatologists. Increasing numbers of comorbidities were associated with poorer values for TJC, SJC, function, and WHO-5.Conclusion.Compared to a matched population, persons with RA present with increased prevalence of numerous comorbidities. Patients with RA and multimorbidity are at risk of insufficient rheumatological care and poorer patient-reported outcomes.
Efficacy of Glucocorticoids and Calcineurin Inhibitors for Anti-aminoacyl-tRNA Synthetase Antibody–positive Polymyositis/dermatomyositis–associated Interstitial Lung Disease: A Propensity Score–matched Analysis
Authors: Hozumi Hironao , Fujisawa Tomoyuki , Nakashima Ran , Yasui Hideki , Suzuki Yuzo , Kono Masato , Karayama Masato , Furuhashi Kazuki , Enomoto Noriyuki , Inui Naoki , Nakamura Yutaro , Mimori Tsuneyo , Suda Takafumi 
Published: January 2019

Objective.The optimal treatment strategy for anti-aminoacyl-tRNA synthetase antibody–positive polymyositis/dermatomyositis-associated interstitial lung disease (anti-ARS-PM/DM-ILD) is yet to be established. We aimed to evaluate the efficacy of glucocorticoids and calcineurin inhibitors (CNI) in patients with ARS-PM/DM-ILD.Methods.Progression-free survival (PFS) and overall survival rates were retrospectively evaluated in 32 consecutive patients with ARS-PM/DM-ILD. Disease progression was defined as deterioration in PM/DM-ILD (including recurrence). Predictive factors associated with PFS were analyzed by Cox hazards analysis. The efficacy of first-line prednisolone (PSL) plus CNI therapy was compared with that of PSL monotherapy using propensity score–matched analysis.Results.Overall, 20 (62.5%) and 12 (37.5%) patients received first-line therapy with PSL + CNI and PSL, respectively. The 2-year PFS and 5-year survival rates in the overall cohort were 68.8% and 96.9%, respectively. On multivariate analysis, arterial oxygen pressure (HR 0.86) and PSL monotherapy (vs PSL + CNI; HR 7.29) showed an independent association with PFS. Baseline characteristics of propensity score-matched PSL + CNI and PSL groups were similar. The 2-year PFS rate was significantly higher in the matched PSL + CNI group than in the matched PSL group. All patients who experienced disease progression during first-line therapy were subsequently treated with second-line therapies. The 5-year survival rates of both the matched PSL + CNI and PSL groups were favorable.Conclusion.Propensity score–matched analysis demonstrated that first-line PSL + CNI therapy for patients with ARS-PM/DM-ILD significantly improved the PFS compared with PSL monotherapy, although there was no significant difference regarding longterm survival.
Report from the Hand Osteoarthritis Working Group at OMERACT 2018: Update on Core Instrument Set Development
Authors: Wittoek Ruth , Kroon Féline P.B. , Kundakci Burak , Abhishek Abhishek , Haugen Ida K. , Berenbaum Francis , Conaghan Philip G. , Ishimori Mariko L. , Smeets Wilma , van der Heijde Désirée , Kloppenburg Margreet 
Published: January 2019

Objective.To evaluate hand osteoarthritis tools for core instrument set development.Methods.For OMERACT 2018, a systematic literature review and advances in instrument validation were presented.Results.Visual analog and numerical rating scales were considered valuable for pain and patient’s global assessment, despite heterogeneous phrasing and missing psychometric evidence for some aspects. The Modified Intermittent and Constant Osteoarthritis Pain scale was lacking evidence. The Michigan Hand Outcomes Questionnaire had advantages above other pain/function questionnaires. The Hand Mobility in Scleroderma scale was valid, although responsiveness was questioned. Potential joint activity instruments were evaluated.Conclusion.The development of the core instrument set is progressing, and a research agenda was also developed.
The 2018 OMERACT Synovial Tissue Biopsy Special Interest Group Report on Standardization of Synovial Biopsy Analysis
Authors: Wechalekar Mihir D. , Najm Aurélie , Veale Douglas J. , Strand Vibeke 
Published: January 2019

Objective.The Outcome Measures in Rheumatology (OMERACT) synovial tissue biopsy (STB) working group initiated an international effort to standardize STB analyses, define consensual items to inform treatment choices, and predict responses in rheumatoid arthritis (RA).Methods.(1) A Delphi survey to determine items for STB analyses. (2) A multicenter retrospective study of STB data in patients with RA posttreatment with biological disease-modifying antirheumatic drugs.Results.The Delphi survey identified 18 STB analyses items. Consensus on histological markers was achieved in the OMERACT 2018 SIG.Conclusion.Six markers were identified for examination in a multicenter study designed to define an OMERACT-endorsed set of STB markers to predict responses to treatment.
Combined Resection and Anterior Transposition of the Inferior Oblique Muscle for the Treatment of Moderate to Large Dissociated Vertical Deviation Associated With Inferior Oblique Muscle Overaction
Authors: Farvardin Majid , Attarzadeh Abbas 
Published: January 2019

ABSTRACT
Purpose: To evaluate the efficacy of combined resection and anterior transposition of the inferior oblique muscle for the treatment of eyes with a moderate to large amount of dissociated vertical deviation (DVD; 10 or more PD) associated with overaction of the inferior oblique muscle.
Patients and Methods: Surgery was performed on 15 eyes of 9 patients with moderate to large DVD associated with overaciion of the inferior oblique muscle. The inferior oblique muscle was disinserted from the sclera and 4 mm of its distal end was resected. It was transposed to a position 1 mm anterior to the lateral border of the inferior rectus muscle insertion. The prism under cover test was used to measure the DVD. Overaction of the inferior oblique muscle was graded on a 4-point scale of 1+ to 4+. The surgical results were evaluated at 1 month and 1 year after surgery.
Results: Preoperativety, the mean DVD measured 16.6 PD. It had decreased to 1.3 PD 1 month after the operation and to 2.6 PD 1 year after the operation. Before the operation, 5 eyes had 4+ and 10 eyes had 3+ inferior oblique muscle overaction. One year after the operation, only 3 eyes had 1+ inferior oblique muscle overaction. None of the patients developed diplopia or hypotropia in the primary position. Mild limitation of elevation has been noticed in 20% of the eyes.
Conclusion: Combined resection and anterior transposition of the inferior oblique muscle is an effective treatment for moderate to large DVD associated with overaciion of the inferior oblique muscle.
J Pediatr Ophthalmol Strabismus 2002;39:268-272.
Decreased Central Corneal Thickness in Children With Down Syndrome
Authors: Evereklioglu Cem , Yilmaz Kutluhan , Bekir Necdet A 
Published: January 2019

ABSTRACT
Purpose: A cross-sectional, masked, case-control study was undertaken to investigate whether central corneal thickness is affected in children with Down syndrome and to focus on its clinical significance.
Participants and Methods: Twenty-eight children with Down syndrome (15 boys and 13 girls) aged 5 to 15 years and 20 age-matched and gendermatched healthy control subjects (1 1 boys and 9 girls) from a similar ethnic background were enrolled in the study. Central corneal thickness was measured by ultrasound pachymetry. Onfy the right eye of each child in each group was included in the statistical analysis. The Mann-Whitney U test was used and a P value of less than .05 was considered significant.
Results: The mean ages of the children with Down syndrome (9.28 ± 3.47 years) and the healthy control subjects (8.75 ± 3.30 years) were comparable (P > .05). Central corneal thickness values were below 500 µ?t? in 19 (67.8%) of the 28 children with Down syndrome, 4 of which were less than 450 µm. However, all central corneal thickness measurements in the control eyes were more than 500 µm. The mean central corneal thickness in the children with Down syndrome was significantly (P < .001 ) less (488.39 ± 39.87 µm) than that in the healthy control subjects (536.25 ± 20.70 µm). Mean keratometric values were significantly (P < .001) higher in the eyes of the children with Down syndrome (46.35 ± 1 .28 D) than in the eyes of the control subjects (43.32 ± 1.15 D).
Conclusions: Children with Down syndrome had a decreased central corneal thickness compared with healthy control subjects. Decreased central cornea! thickness may give an artificially low intraocular pressure measurement by applanation tonometry. Central corneal thickness must be considered when developing approaches for keratorefractive treatment of patients with Down syndrome.
J Pediatr Opthalmol Strabismus 2002;39:274-277.
An Italian Family Affected by Autosomal Dominant Microcephaly With Chorioretinal Degeneration
Authors: Simonelli Francesca , Testa Francesco , Nesti Anna , de Crecchio Giuseppe , Bifani Mario , Cavaliere Maria Luisa , Rinaldi Ernesto , Rinaldi Maria Michela 
Published: January 2019

ABSTRACT
Purpose: We studied an Italian family affected by the autosomal dominant form of microcephaly and Chorioretinal degeneration that was characterized by various degrees of clinical expression.
Methods: An ophthalmologic examination, including visual acuity, visual field testing, an electroretinogram, and fundus photography, and a neurologic examination, including neurodevelopmental status and neuroimaging studies, were performed for all subjects. Skeletal radiography, chromosome studies, and serologie investigations were also performed.
Results: In this family, only two of the six affected members had an association of microcephaly, myopia, and chorioretinal degeneration. The other family members showed microcephaly, slight mental retardation, and short stature, but not chorioretinopathy.
Conclusions: The significant finding in members from this dominant pedigree of microcephaly was the association of short stature and high myopia, heretofore seen only in families with recessive microcephaly. These findings could be useful for genetic counseling in the apparently isolated forms of microcephaly with chorioretinopathy.
J Pediatr Ophthalmol Strabismus 2002;39:288292.
Long-Term Follow-Up of L-dopa Treatment in Children With Amblyopia
Authors: Leguire Lawrence E , Komaromy Kelly L , Nairus Theresa M , Rogers Gary L 
Published: January 2019

ABSTRACT
Purpose: To assess regression of visual acuity in children who previously participated in three longitudinal studies of therapy with levodopa-carbidopa (L-dopa) plus occlusion for amblyopia.
Subjects and Methods: Thirty (91%) of 33 subjects contacted who participated in three similar 7-week, longitudinal dosing studies returned for follow-up. The three previous studies were undertaken approximately 27 (study 1 ), 2 1 (study 2), and 9 (study 3) months prior to this follow-up test session. All subjects received L-dopa for 7 weeks combined with part-time occlusion of the dominant eye after the termination of standard, occlusion only, therapy. Some subjects in study 2 received L-dopa without occlusion. Sixteen subjects with amblyopia who received occlusion only served as a control group.
Results: Subjects who received L-dopa plus occlusion demonstrated significant, but similar, amounts of regression of visual acuity from the end of their respective Ldopa studies to the follow-up test session. Subjects in study 1 regressed 1.3 lines, subjects in study 2 regressed 1.5 lines, and subjects in study 3 regressed 1.4 lines. The control group regressed, on average, 1 .1 lines. This was similar to the overall regression found in the groups receiving L-dopa plus occlusion. Subjects in study 2 who received L-dopa without occlusion regressed 2.1 lines, significantly more than the occlusion only group or the L-dopa plus occlusion group.
Conclusions: Children with amblyopia show similar amounts of regression of visual acuity after therapy with L-dopa plus occlusion and after therapy with occlusion only. Given that therapy with L-dopa plus occlusion initially improved visual acuity by approximately 1.7 lines following the termination of standard therapy, L-dopa plus occlusion may reset baseline visual acuity and lead to long-term improvement in visual acuity after recidivism.