bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineered receptors for soluble cellular communication and disease sensing
Authors: Piraner Dan I. , Abedi Mohamad H. , Duran Gonzalez Maria J. , Chazin-Gray Adam , Lin Annie , Zhu Iowis , Ravindran Pavithran T. , Schlichthaerle Thomas , Huang Buwei , Bearchild Tyler H. , Lee David , Wyman Sarah , Jun Young-wook , Baker David , Roybal Kole T. 
Published: November 2024

Abstract
Despite recent advances in mammalian synthetic biology, there remains a lack of modular synthetic receptors that can robustly respond to soluble ligands and, in turn, activate bespoke cellular functions. Such receptors would have extensive clinical potential to regulate the activity of engineered therapeutic cells, but so far only receptors against cell-surface targets have approached clinical translation1. To address this gap, here we adapt a receptor architecture called the synthetic intramembrane proteolysis receptor (SNIPR) for activation by soluble ligands. Our SNIPR platform can be activated by both natural and synthetic soluble factors, with notably low baseline activity and high fold activation, through an endocytic, pH-dependent cleavage mechanism. We demonstrate the therapeutic capabilities of the receptor platform by localizing the activity of chimeric antigen receptor (CAR) T cells to solid tumours in which soluble disease-associated factors are expressed, bypassing the major hurdle of on-target off-tumour toxicity in bystander organs. We further apply the SNIPR platform to engineer fully synthetic signalling networks between cells orthogonal to natural signalling pathways, expanding the scope of synthetic biology. Our design framework enables cellular communication and environmental interactions, extending the capabilities of synthetic cellular networking in clinical and research contexts.
A comprehensive human embryo reference tool using single-cell RNA-sequencing data
Authors: Zhao Cheng , Plaza Reyes Alvaro , Schell John Paul , Weltner Jere , Ortega Nicolás M. , Zheng Yi , Björklund Åsa K. , Baqué-Vidal Laura , Sokka Joonas , Trokovic Ras , Cox Brian , Rossant Janet , Fu Jianping , Petropoulos Sophie , Lanner Fredrik 
Published: November 2024

Abstract
Stem cell-based embryo models offer unprecedented experimental tools for studying early human development. The usefulness of embryo models hinges on their molecular, cellular and structural fidelities to their in vivo counterparts. To authenticate human embryo models, single-cell RNA sequencing has been utilized for unbiased transcriptional profiling. However, an organized and integrated human single-cell RNA-sequencing dataset, serving as a universal reference for benchmarking human embryo models, remains unavailable. Here we developed such a reference through the integration of six published human datasets covering development from the zygote to the gastrula. Lineage annotations are contrasted and validated with available human and nonhuman primate datasets. Using stabilized Uniform Manifold Approximation and Projection, we constructed an early embryogenesis prediction tool, where query datasets can be projected on the reference and annotated with predicted cell identities. Using this reference tool, we examined published human embryo models, highlighting the risk of misannotation when relevant references are not utilized for benchmarking and authentication.
Revisiting the scorpion central nervous system using microCT
Authors: Loria Stephanie F. , Ehrenthal Valentin L. , Esposito Lauren A. 
Published: November 2024

AbstractThe central nervous system (CNS) of Chelicerata has remained conserved since the Cambrian, yet few studies have examined its variability within chelicerate orders including Scorpiones. The scorpion CNS comprises the prosomal ganglion and opisthosomal ventral nerve cord. We visualize the scorpion CNS with microCT, explore morphological variation across taxa, compare the scorpion CNS to other arachnids, and create a terminology glossary and literature review to assist future studies. Six scorpion species were microCT scanned. Scan quality varied and most structures in the prosomal ganglion could only be observed in Paruroctonus becki (Vaejovidae). Major nerves and the first opisthosomal ganglion were visible in nearly all taxa. We present the most detailed 3D-rendering of the scorpion prosomal ganglion to date. Our results corroborate existing research and find the scorpion CNS to be conserved. Nearly all structures reported previously in the prosomal ganglion were located in similar positions in P. becki, and nerve morphology was conserved across examined families. Despite similarities, we report differences from the literature, observe taxonomic variation in prosomal ganglion shape, and confirm positional variation for the first opisthosomal ganglion. This study serves as a starting point for microCT analysis of the scorpion CNS, and future work should include more distantly related, size variable taxa to better elucidate these findings.
A new scoring system to predict fatal accidents in General Aviation and to facilitate emergency control centre response
Authors: Hinkelbein Jochen , Hippler Catherina , Liebold Felix , Schmitz Jan , Rothschild Markus , Schick Volker 
Published: November 2024

AbstractNumerous accidents occur with General Aviation aircraft every year. To date, pre-emptive prediction of survival or death is impossible. The current study aims to identify significant factors elementary to predict survival after General Aviation (GA) aircraft accidents. The Implementation of a scoring system, including these factors, may facilitate emergency control centre response. Data of flight accidents over a 20-year period (extracted from the German Federal Bureau of Aircraft Accident Investigation [BFU]) was analysed for fixed-wing motorized small aircrafts below 5,700 kg MTOW. Factors of interest were analysed using Chi2- and Mann–Whitney-U-Tests. Logistic regression was used to establish a score to calculate the probability of a fatal outcome after an aircraft accident. The BFU lists 1,595 GA aircraft accidents between 2000 and 2019. The factors “third quarter of the year” (p = 0.04), “last quarter of the year” (p = 0.002), “fire” (p < 0.0001), “distance from airport > 10 km” (p < 0.0001), “landing” (p < 0.0001) and “cruise” (p < 0.0001), significantly correlated positively or negatively with a fatal outcome. “Take-off”, “approach”, “month”, “day of the week”, “persons on board above three”, “night-time” and “icing conditions” showed no significant correlation. Using logistic regression “third quarter of the year” and “cruise” were excluded when using the B-STEP method. Including the four significant parameters, the score showed a strong effect with f2 = 0.709. The analysis of GA aircraft accidents in Germany enabled the identification of relevant factors and establishment of a new scoring system for survival prediction after small aircrafts accidents below 5,700 kg MTOW. The implementation of the scoring system in emergency control centres in the context of digital development and artificial intelligence can improve emergency response planning and distribution.
Immuno-physiological role of exogenous enzymes supplementation in heat stressed growing calves
Authors: Eid Sherif Yousif , Emara Sana Sayed , Sharaf Ahmed Kamel , Ahmed-Farid Omar A. , El-Zaher Hussein Mostafa 
Published: November 2024

AbstractTwenty Crossbred (Baladi× Brown-Swiss) male growing-calves, 6 to 8 months old, weighing an average of 115 to 125 kg were subjected for evaluating ZADO supplementary effects on blood biochemical parameters, antioxidant status, thyroid activity, and growth efficiency of calves under semi-arid conditions. The experimental units were pinned equally in two groups. The control (G1) fed the basal ration, and the 2nd, treatment group (G2) fed the basal ration + a daily supplement of 10 g ZADO (exogenous enzymes) calf− 1 day− 1 in a powder form mixed well with ration. The results showed that ZADO treatment reduced (P < 0.001) each of respiration rate (RR), rectal temperature (RT), serum malondialdehyde (MDA), urea, creatinine, and cortisol. Also, caused a marked decline in oxidized glutathione (GSSG) and alanine aminotransferase (ALT) activities. However, supplemented ZADO to growing calves diets improved antioxidant status including reduced glutathione (GSH) and superoxide dismutase (SOD) activities, total antioxidant capacity, serum total protein, albumin, and globulin, as well as IgG and IgM besides. ZADO enhanced (P < 0.01) thyroid activity, and feed efficacy parameters. Finally, supplementing growing calves with ZADO under semi-arid circumstances alleviates the heat stress effect, and leads to an improvement in calves’ growth performance.
Innovative application of green surfactants as eco-friendly scale inhibitors in industrial water systems
Authors: Khamis E. , Abd-El-Khalek D. E. , Fawzy Manal , Soliman Kamal A. , Abdel-Gaber A. M. , Anwar J. M. 
Published: November 2024

AbstractScale deposition poses significant challenges in various industrial utilities, necessitating the development of eco-friendly scale inhibitors in line with environmental regulations. This study investigates the potential of two natural surfactants, Casein and Rhamnolipid, as innovative inhibitors for calcium carbonate (CaCO3) scale formation, offering an alternative to traditional water treatment chemicals. The anti-scaling characteristics of these two green surfactants were performed using conductivity and electrochemical impedance spectroscopy (EIS) techniques. Additionally, X-ray diffraction (XRD) and scanning electron microscopy (SEM) were utilized to analyze the morphology of CaCO3 crystals and understand the structural changes induced by surfactant interaction. The results revealed that Rhamnolipids significantly outperform Casein in suppressing scale formation, attributed to the adsorption of their multiple functional groups onto scale microcrystals. This adsorption modifies the crystal structure and inhibits further growth. Computational studies were employed to investigate the inhibition mechanism of these surfactants. The spatial and electrical configurations of optimal molecular structures are also analyzed using the Density Functional Theory (DFT) approach. Monte Carlo simulations on the CaCO3 (104) surface demonstrated that Rhamnolipids exhibit superior inhibition compared to Casein, as evidenced by their higher adsorption energy, indicating a more stable binding to the surface. Overall, this research highlights the potential of these natural surfactants as sustainable scale inhibitors, particularly in industries such as food and pharmaceuticals, thereby contributing to environmentally friendly water treatment solutions.
Energy-resolved neutron imaging study of a Japanese sword signed by Bishu Osafune Norimitsu
Authors: Oikawa Kenichi , Matsumoto Yoshihiro , Watanabe Kenichi , Sato Hirotaka , Parker Joseph Don , Shinohara Takenao , Kiyanagi Yoshiaki 
Published: November 2024

Abstract
Our research focuses on elucidating the crystallographic structure of Japanese swords in a nondestructive manner using the neutron imaging instrument RADEN at the Materials and Life Science Experimental Facility of the Japan Proton Accelerator Research Complex (J-PARC). We developed an analysis method combining wavelength-resolved Bragg-edge imaging and wavelength-selective neutron tomography with a new strategy and applied it to an approximately 45-cm blade length Japanese sword signed by Bishu Osafune Norimitsu. Computed tomography was performed, and the three-dimensional analysis captured the characteristic internal structure of Kobuse. Kobuse is the most famous steel-combining structure of Japanese swords, where an outer steel with high-carbon content (Kawagane) covers a core steel with low-carbon content (Shingane). The crystallite size distribution obtained through Bragg-edge analysis could consistently explain the internal structure of two steels observed in neutron tomograms. Our nondestructive imaging revealed deep hardening, forming a wavy pattern more than 5 mm from the cutting edge.
Mummy of a juvenile sabre-toothed cat Homotherium latidens from the Upper Pleistocene of Siberia
Authors: Lopatin A. V. , Sotnikova M. V. , Klimovsky A. I. , Lavrov A. V. , Protopopov A. V. , Gimranov D. O. , Parkhomchuk E. V. 
Published: November 2024

AbstractThe frozen mummy of the large felid cub was found in the Upper Pleistocene permafrost on the Badyarikha River (Indigirka River basin) in the northeast of Yakutia, Russia. The study of the specimen appearance showed its significant differences from a modern lion cub of similar age (three weeks) in the unusual shape of the muzzle with a large mouth opening and small ears, the very massive neck region, the elongated forelimbs, and the dark coat color. Tomographic analysis of the mummy skull revealed the features characteristic of Machairodontinae and of the genus Homotherium. For the first time in the history of paleontology, the appearance of an extinct mammal that has no analogues in the modern fauna has been studied.
Facilitators and Barriers to Integrating Patient-Generated Blood Pressure Data into Primary Care EHR Workflows
Authors: Canfield Shannon M. , Koopman Richelle J. 
Published: November 2024

Abstract
Background Evidence supports using patient-generated blood pressure data for better outcomes in hypertension management. However, obstacles like dealing with home-generated paper data sets and questions of validity slowed the meaningful incorporation of home blood pressure into clinical care. As clinicians value patient data more, reliance on digital health solutions for data collection and shared decision-making grows.
Objectives The purpose of this study is to evaluate the design and early implementation of an electronic health record (EHR)-based data visualization tool and explore the barriers or facilitators to integrating) patients' home blood pressure data into the electronic workflow in the clinical setting. Findings can inform potential next steps for implementation and provide recommendations for leveraging patient-generated health data (PGHD) in hypertension management.
Methods We qualitatively explored pre- and early-implementation factors for integrating PGHD into clinicians' EHR interfaces intended to support shared decision-making using the Consolidated Framework for Implementation Research (CFIR). We collected data in the form of notes and transcripts from clinician focus groups, administrative leadership feedback sessions, research team observations, and recurring team meetings. This study took place at a midwestern academic health center.
Results We identify implementation facilitating factors, adoption considerations, and next steps across CFIR domains focusing on large-scale implementation. Key recommendations include aligning internal and external priorities, empowering champions to facilitate uptake, using intuitive design, and anticipating and planning for unintended consequences.
Conclusion These findings can guide future efforts to include PGHD in workflows, thus enhancing shared decision-making and laying the groundwork for larger implementations. Understanding the implementation barriers and facilitators to connect PGHD to clinician apps in the EHR workspace can promote their adoption and maintenance.
Trans* & gender identity in the premodern Mediterranean
Authors: McDonough Susan , Armstrong-Partida Michelle 
Published: November 2024

Abstract
This paper explores the intersection and imbrication of transness and Mediterraneaness in the premodern period. How did Mediterranean mobility, spaces, and creativity inform and make possible the ‘transing’ of gender? Re-examining previously considered sources with the benefit of recent scholarship on archival silences and trans history, we suggest that Mediterranean culture, migration, local community, and race shaped possibilities for transgender people. Prioritizing the agency of people who lived non-binary and trans lives, we make them legible to a contemporary audience while refraining from imposing our own labels upon them. The messy and contradictory lives of our subjects show the complexity of personhood and identity. We consider unrecorded suffering and reflect on how the physical bodies of those punished for transgressing gender norms were inscribed with meaning that resonated with hegemonic constructions of sexuality and identity. We center the bodies, identities, and experiences of trans people rather than the elite male discourses of a heteronormative society.
Upconversion of phonon modes into microwave photons in a lithium niobate bulk acoustic wave resonator coupled to a microwave cavity
Authors: Parashar S. , Campbell W. M. , Bourhill J. , Ivanov E. , Goryachev M. , Tobar M. E. 
Published: November 2024

The coupling between acoustic vibrations in a lithium niobate bulk acoustic wave resonator and microwave photons of a re-entrant microwave cavity was investigated at a temperature close to 4 K. Coupling was achieved by placing the acoustic resonator in the location of the re-entrant cavity electric field maxima in a symmetric “split-post” configuration with a large overlap between the microwave field and the acoustic mode, allowing acoustic modulations of the microwave frequency. We show that the acoustic modes in this setup retain large inherent quality factors of greater than 106. A maximum optomechanical coupling rate was determined to be g0 = 0.014 mHz, four orders of magnitude larger than previous results obtained using a quartz BAW at 4 K in a similar experimental setup but using a single post-re-entrant cavity resonator.
Development of a compact and portable diamond-based detection system for dosimetry and microdosimetry in ion beam therapy
Authors: Verona C. , Fabbri A. , Fazzi A. , Bianchi L. , Conte V. , Petringa G. , Raso A. , Rinati G. Verona 
Published: November 2024

Ion beam therapy techniques have advanced significantly in the past two decades. However, the development of dosimetric verification methods has lagged. Traditional dosimetry, which offers a macroscopic view of the absorbed dose, fails to address the micrometric-scale stochastic effects crucial for understanding biological responses. To bridge this gap, microdosimeters are used to assess physical quantities correlated with radiation effects. This work reports on the design and testing of a novel detection system based on synthetic single crystal diamond. The system is capable of simultaneously performing dosimetric and microdosimetric characterizations of clinical ion beams. The detector incorporates two active components configured as diamond Schottky diodes, both integrated on a single crystal diamond substrate. In particular, one very small element (sensitive area 0.0078 mm2) was designed to evaluate microdosimetric metrics, while the other large one (sensitive area 4.2 mm2) was designed to measure the absorbed dose to water. Diamond detectors were characterized using the ion beam induced charge (IBIC) technique, employing a 1 MeV protons microbeam. The IBIC map of the diamond detector shows two distinct sensitive areas with quite uniform sensitivity, well contained within the metallic contact regions. Dedicated front-end electronic circuits were designed and implemented for both the dosimetric and microdosimetric signals. These circuits, along with the integrated diamond detector, were embedded in an aluminum waterproof housing to minimize electronic interference. This configuration enables a compact, portable setup compatible with water phantoms. Laboratory tests with alpha particles yielded promising results, demonstrating stable and reproducible responses with a good signal-to-noise ratio.
EFL students’ beliefs and practices regarding pronunciation self-regulated learning
Authors: Nguyen Loc Tan 
Published: November 2024

Abstract
The past three decades have witnessed growing interest in pronunciation research focused on a range of topics such
as the effects of explicit phonetic instruction on second/foreign language (L2) learners’ pronunciation uptake, teachers’
pronunciation teaching practices and beliefs, pronunciation representation in prescribed textbooks, and L2 students’ perceptions
of their teachers’ pronunciation instruction. However, L2 learners’ beliefs and practices regarding pronunciation self-regulated
learning (PSRL) is under-explored. The present study examines how Vietnamese tertiary EFL learners practice PSRL and what beliefs
they hold about it. Data were collected from a questionnaire completed by 215 EFL students at a Vietnamese public university and
semi-structured interviews with ten of the students. Results showed that the students demonstrated a strong commitment to
self-practicing English pronunciation and that their PSRL practices centered on segmental features through listen-and-repeat
activities from YouTube pronunciation lesson videos and textbook audio-recordings. The study further showed that the students
believed PSRL had a positive impact on their own pronunciation but called for phonetic instruction to guide their self-practice,
followed by teachers giving feedback on their pronunciation. Implications for L2 pronunciation instruction and PSRL are
discussed.
Spin photo detector by using a CoFeB magnetic tunnel junction
Authors: Shibata Tetsuya , Mizuno Tomohito , Nojiri Takeshi , Yamane Takekazu , Hirata Yusho , Zhang Weihao , Fukuzawa Hideaki , Kasatani Yuichi , Tsukamoto Arata 
Published: November 2024

Abstract
Ultra-fast photoelectric conversion devices are a crucial element in photonics applications and are the subject of intense research and development. In conventional high-speed photo detectors, photoelectric charge generation in semiconductors is rooted in a mechanism that directly outputs current. Owing to the dilemma of the amount of generated charge and high-speed output, it is being faced with fundamental difficult to achieve a higher response. Spin order, rather than charge generation, also exists as a phenomenon that enables ultrafast photoresponses, and magnetic tunnel junction (MTJ) are well-known high-speed electrical detection elements for magnetic states. In this study, we experimentally confirmed the magnetic switching of photoresponsive MTJ using a practical single CoFeB free layer with a high MR ratio of 80% by irradiation with laser light. Furthermore, we experimentally confirmed the operation of the reversible photo detector, and its rise time reached an ultrafast speed of 20 ps. We named it spin photo detector and it has huge potential applications that require ultrafast photo detection.
Variational inference of effective range parameters for 3He−4He scattering
Authors: Burnelis Andrius , Kejzlar Vojtech , Phillips Daniel R 
Published: November 2024

Abstract
We use two different methods, Monte Carlo sampling and variational inference (VI), to perform a Bayesian calibration of the effective-range parameters in 3He–4He elastic scattering. The parameters are calibrated to data from a recent set of 3He–4He elastic scattering differential cross section measurements. Analysis of these data for E
lab ≤ 4.3 MeV yields a unimodal posterior for which both methods obtain the same structure. However, the effective-range expansion amplitude does not account for the 7/2− state of 7Be so, even after calibration, the description of data at the upper end of this energy range is poor. The data up to E
lab = 2.6 MeV can be well described, but calibration to this lower-energy subset of the data yields a bimodal posterior. After adapting VI to treat such a multi-modal posterior we find good agreement between the VI results and those obtained with parallel-tempered Monte Carlo sampling.