bluuebunny/crossref_metadata_2025_split huggingface dataset:
High throughput photogrammetric measurement of morphological traits in free-ranging phototropic insects
Authors: Mungee Mansi , Athreya Ramana 
Published: July 2019

AbstractRemote measurement of morphological traits in free-ranging animals is very useful for many studies, but such non-invasive photogrammetric methods are limited to large mammals and have yet to be successfully applied to insects which dominate terrestrial ecosystem diversity and dynamics. Currently, insect traits are measured using collected specimens; the process of collection and maintenance of specimens can impose a heavy and unnecessary cost when such specimens themselves are not needed for the study.We propose a rapid, simple, accurate, and semi-automated method for high-throughput morphometric measurements of phototropic insects from shape and size calibrated digital images without having to collect a specimen. The method only requires inexpensive, off-the-shelf, consumer equipment and freely available programming (R) and image processing (ImageMagick) tools.We demonstrate the efficacy of the method using a data set of 3675 images of free-ranging hawkmoths (Lepidoptera:Sphingidae) attracted to a light screen. Comparison of trait values from a subset of these images with direct measurements of specimens using a scale showed a high degree of correspondence. We have also identified several error metrics which help in assessing the method in an objective manner.Although this method was developed for nocturnal phototropic insects, it can be used for any other (small) animal that can be imaged on a simple graph paper. While this technique will be generally useful for a variety of studies of insect traits, we suggest that it is particularly suited as a commensal on multi-epoch and multi-location population monitoring of insects in the context of climate and land-use change, where repeated sampling obviates the necessity of collecting specimen every time. It will help in accumulating a large amount of reliable trait data on hundreds of thousands of individual insects without an overwhelming expenditure on collection, handling, and maintenance of specimens.
Circular RNAs in the ageing African turquoise killifish
Authors: Metge Franziska , Kim Yumi , Boucas Jorge , Dieterich Christoph , Valenzano Dario Riccardo 
Published: July 2019

AbstractCircRNAs are a subgroup of RNAs which form a circular molecule. During the splicing process the 3’ splice donor loops back to form a covalent bond with an upstream 5’ splice acceptor instead of the downstream 5’ acceptor. The majority of circRNAs are non-coding splice isoforms of protein coding genes showing tissue and time specific expression. Because circRNAs have no 5’ cap nor 3’ poly-A tail, they degrade slower than their linear host genes. Only few studies were able to show a specific function for circRNAs.In this work we sequenced 23 samples from three tissues (brain, muscle, and gut) at three (two for gut) time points throughout the life of the naturally short-lived African turquoise killifish (Nothobranchius furzeri). We identified 1810 unique circRNAs, half of which are conserved with humans and mice. With this study we provide a comprehensive atlas to the circRNA landscape in the ageing African turquoise killifish, which serves as a novel resource to the circRNA as well as the ageing community.
MRI-based Parcellation and Morphometry of the Individual Rhesus Monkey Brain: a translational system referencing a standardized ontology
Authors: Rushmore R. Jarrett , Bouix Sylvain , Kubicki Marek , Rathi Yogesh , Yeterian Edward H. , Makris Nikos 
Published: July 2019

AbstractThe rhesus macaque is the closest animal model to the human, and investigations into the brain of the rhesus monkey has shed light on the function and organization of the primate brain at a scale and resolution not yet possible in studies of the human brain A cornerstone of the linkage between non-human primate and human studies of the brain is magnetic resonance imaging, which allows for an association to be made between the detailed structural and physiological analysis of the non-human primate and that of the human brain. To further this end, we present a novel parcellation system for the rhesus monkey brain, referred to as the monkey Harvard Oxford Atlas (mHOA) which is based on the human Harvard-Oxford Atlas (HOA) and grounded in an ontological and taxonomic framework. Consistent anatomical features were used to delimit and parcellate brain regions in the macaque, which were then categorized according to functional systems. This system of parcellation will be expanded with advances in technology and like the HOA, will provide a framework upon which the results from other experimental studies (e.g., functional magnetic resonance imaging (fMRI), physiology, connection, graph theory) can be interpreted.
BOLD differences normally attributed to inhibitory control predict symptoms, not task-directed inhibitory control in ADHD
Authors: Chevrier Andre , Schachar Russell J. 
Published: July 2019

AbstractBackgroundAltered brain activity that has been observed in attention deficit hyperactivity disorder (ADHD) while performing cognitive control tasks like the stop signal task (SST), has generally been interpreted as reflecting either weak (under-active) or compensatory (over-active) versions of the same functions as in healthy controls. If so, then regional activities that correlate with the efficiency of inhibitory control (i.e. stop signal reaction time, SSRT) in healthy subjects should also correlate with SSRT in ADHD. Here we test the alternate hypothesis that BOLD differences might instead reflect the redirection of neural processing resources normally used for task-directed inhibitory control, toward actively managing symptomatic behavior. If so, then activities that correlate with SSRT in TD should instead correlate with inattentive and hyperactive symptoms in ADHD.MethodsWe used fMRI in 14 typically developing (TD) and 14 ADHD adolescents performing the SST, and in a replication sample of 14 healthy adults. First we identified significant group BOLD differences during all phases of activity in the SST (i.e. warning, response, reactive inhibition, error detection and post-error slowing). Next, we correlated these phases of activity with SSRT in TD, and with SSRT, inattentive and hyperactive symptom scores in ADHD. We then identified whole brain significant correlations in regions of significant group difference in activity.ResultsOnly three regions of significant group difference were correlated with SSRT in TD and replication groups (left and right inferior frontal gyri (IFG) during error detection, and hypothalamus during post-error slowing). Consistent with regions of altered activity managing symptomatic behavior instead of task-directed behavior, left IFG correlated with greater inattentive score, right IFG correlated with lower hyperactive score, and hypothalamus correlated with greater inattentive score and oppositely correlated with SSRT compared to TD.ConclusionsResults are consistent with stimuli that elicit task-directed integration of neural processing in healthy subjects, instead directing integrated function towards managing symptomatic behavior in ADHD. The ability of the current approach to determine whether altered neural activities reflect comparable functions in ADHD and control groups has broad implications for the development and monitoring of therapeutic interventions.
Impact of Ferumoxytol Magnetic Resonance Imaging on the Rhesus Macaque Maternal-Fetal Interface
Authors: Nguyen Sydney M. , Wiepz Gregory J. , Schotzko Michele , Simmons Heather A. , Mejia Andres , Ludwig Kai D. , Zhu Ante , Brunner Kevin , Hernando Diego , Reeder Scott B. , Wieben Oliver , Johnson Kevin , Shah Dinesh , Golos Thaddeus G. 
Published: July 2019

AbstractFerumoxytol is a superparamagnetic iron oxide nanoparticle (SPION) used off-label as an intravascular magnetic resonance imaging (MRI) contrast agent. Additionally, ferumoxytol-uptake by macrophages facilitates detection of inflammatory sites by MRI through ferumoxytol-induced image contrast changes. Therefore, ferumoxytol-enhanced MRI holds great potential for assessing vascular function and inflammatory response, critical to determine placental health in pregnancy. This study sought to assess the fetoplacental unit and selected maternal tissues, pregnancy outcomes, and fetal well-being after ferumoxytol administration. In initial developmental studies, pregnant rhesus macaques were imaged with and without ferumoxytol administration. Pregnancies went to term with vaginal delivery and infants showed normal growth rates compared to control animals born the same year that did not undergo MRI. To determine the impact of ferumoxytol on the maternal-fetal interface, fetal well-being, and pregnancy outcome, four pregnant rhesus macaques at ∼100 gd (gestational day) underwent MRI before and after ferumoxytol administration. Collection of the fetoplacental unit and selected maternal tissues was performed 3-4 days following ferumoxytol administration. A control group that did not receive ferumoxytol or MRI was used for comparison. Iron levels in fetal and maternal-fetal interface tissues did not vary between groups. There was no significant difference in tissue histopathology with or without exposure to ferumoxytol, and no effect on placental hormone secretion. Together, these results suggest that the use of ferumoxytol and MRI in pregnant rhesus macaques will not introduce a detectable risk to the mother or fetus at the time of imaging or up to one year following normal vaginal delivery.Summary SentenceFerumoxytol magnetic resonance imaging for non-invasive pregnancy monitoring of the rhesus macaque does not impact histopathology or iron content of the maternal-fetal interface.
From reporters to endogenous genes: the impact of the first five codons on translation efficiency in Escherichia coli
Authors: Moreira Mariana H. , Barros Géssica C. , Requião Rodrigo D. , Rossetto Silvana , Domitrovic Tatiana , Palhano Fernando L. 
Published: July 2019

ABSTRACTTranslation initiation is a critical step in the regulation of protein synthesis, and it is subjected to different control mechanisms, such as 5’ UTR secondary structure and initiation codon context, that can influence the rates at which initiation and consequentially translation occur. For some genes, translation elongation also affects the rate of protein synthesis. With a GFP library containing nearly all possible combinations of nucleotides from the 3rd to the 5th codon positions in the protein coding region of the mRNA, it was previously demonstrated that some nucleotide combinations increased GFP expression up to four orders of magnitude. While it is clear that the codon region from positions 3 to 5 can influence protein expression levels of artificial constructs, its impact on endogenous proteins is still unknown. Through bioinformatics analysis, we identified the nucleotide combinations of the GFP library in Escherichia coli genes and examined the correlation between the expected levels of translation according to the GFP data with the experimental measures of protein expression. We observed that E. coli genes were enriched with the nucleotide compositions that enhanced protein expression in the GFP library, but surprisingly, it seemed to affect the translation efficiency only marginally. Nevertheless, our data indicate that different enterobacteria present similar nucleotide composition enrichment as E. coli, suggesting an evolutionary pressure towards the conservation of short translational enhancer sequences.
Biochemical reconstitution of branching microtubule nucleation
Authors: Alfaro-Aco Raymundo , Thawani Akanksha , Petry Sabine 
Published: July 2019

AbstractMicrotubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways still need to be identified along with their mode of action. Here, using purified Xenopus laevis proteins we biochemically reconstitute branching microtubule nucleation, a nucleation pathway where microtubules originate from pre-existing microtubules, which is essential for spindle assembly and chromosome segregation. We found that besides the microtubule nucleator gamma-tubulin ring complex (γ-TuRC), the two branching effectors augmin and TPX2 are required to efficiently nucleate branched microtubules. Specifically, TPX2 generates regularly-spaced patches that recruit augmin and γ-TuRC to microtubules, which then nucleate new microtubules at preferred branching angles of less than 90 degrees. Our work demonstrates how γ-TuRC is brought to its nucleation site for branching microtubule nucleation. It provides a blueprint for other microtubule nucleation pathways and for generating a particular microtubule architecture by regulating microtubule nucleation.
RP3V kisspeptin neurons mediate neuroprogesterone induction of the luteinizing hormone surge in female rat
Authors: Delhousay Lauren K. , Chuon Timbora , Mittleman-Smith Melinda , Micevych Paul , Sinchak Kevin 
Published: July 2019

ABSTRACTTo induce ovulation, neural circuits are sequentially activated by estradiol and progesterone. In female rodents, estradiol-induced neuroprogesterone, synthesized in astrocytes, is essential for the luteinizing hormone (LH) surge and subsequently, ovulation. However, the gonadotropin-releasing hormone (GnRH) neurons do not express the steroid receptors necessary for reproduction: progesterone receptors (PGR) or estrogen receptor-α (ERα). Steroid information is transduced by rostral periventricular (RP3V) kisspeptin neurons that express ERα and PGR and innervate GnRH neurons in the diagonal band of Broca (DBB) and the medial septum. In this study, we tested the hypothesis that estradiol induced neuroprogesterone needed for the LH surge is mediated by kisspeptin. Neuroprogesterone synthesis was inhibited with aminoglutethimide (AGT; s.c.) in 17β-estradiol benzoate (EB)-primed, ovariectomized (ovx) and adrenalectomized (adx) rats. Kisspeptin-10 (20 nmol/µl) was infused into the DBB, trunk blood was collected 53 hours post-EB injection, and serum LH levels were analyzed by ELISA. AGT inhibition of neuroprogesterone synthesis blocked the EB-induced LH surge. Subsequent treatment with either progesterone or DBB kisspeptin-10 infusion restored the LH surge. Kisspeptin restored the LH surge, which was blocked by DBB infusion of kisspeptin receptor (GPR54) antagonist (kisspeptin-234). Finally, knockdown of kisspeptin protein levels in the RP3V with kisspeptin antisense oligodeoxynucleotide (ODN) significantly lowered LH levels in EB-primed rats compared to scrambled ODN, demonstrating the importance of endogenous RP3V kisspeptin for the LH surge. These results support the hypothesis that neuroprogesterone induces both kisspeptin release from RP3V neurons impacting the LH surge.
Automated data extraction from historical city directories: the rise and fall of mid-century gas stations in Providence, RI
Authors: Bell Samuel , Marlow Thomas , Wombacher Kai , Hitt Anina , Parikh Neev , Zsom Andras , Frickel Scott 
Published: July 2019

AbstractThe location of defunct environmentally hazardous businesses like gas stations has many implications for modern American cities. To track down these locations, we present the directoreadr code (github.com/brown-ccv/directoreadr). Using scans of Polk city directories from Providence, RI, directoreadr extracts and parses business location data with a high degree of accuracy. The image processing pipeline ran without any human input for 94.4% of the pages we examined. For the remaining 5.6%, we processed them with some human input. Through hand-checking a sample of three years, we estimate that ~94.6% of historical gas stations are correctly identified and located, with historical street changes and non-standard address formats being the main drivers of errors. As an example use, we look at gas stations, finding that gas stations were most common early in the study period in 1936, beginning a sharp and steady decline around 1950. We are making the dataset produced by directoreadr publicly available. We hope it will be used to explore a range of important questions about socioeconomic patterns in Providence and cities like it during the transformations of the mid-1900s.
Eyeing the past: synchrotron µ-XANES and XRF imaging of tarnish distribution on 19th century daguerreotypes
Authors: Kozachuk M. S. , Sham T. K. , Martin R. R. , Nelson A. J. , Coulthard I. 
Published: July 2019

Louis-Jacques-Mandé Daguerre introduced the first successful photographic process, the daguerreotype, in 1839. Tarnished regions on daguerreotypes supplied by the National Gallery of Canada were examined using scanning electron microscopy energy-dispersive X-ray spectroscopy and synchrotron-radiation analysis. Synchrotron X-ray fluorescence imaging visualized the distribution of sulfur and chlorine, two primary tarnish contributors, and showed that they were associated with the distribution of image particles on the surface. X-ray absorption near-edge structure spectroscopy determined the tarnish to be primarily composed of AgCl and Ag2S. Au2S, Au2SO4, HAuCl4and HgSO4were also observed to be minor contributors. Environmental contamination may be a source of these degradation compounds. Implications of these findings will be discussed.
Factors influencing a long-term relationship between healthcare providers and patients – perspectives of patients at a public regional hospital, Ghana
Authors: Adomah-Afari Augustine , Doris Darkoa Mantey Doris , Awuah-Werekoh Kwasi 
Published: July 2019

Purpose
The purpose of this paper is to determine the factors that influence patients’ long-term relationship with healthcare providers in healthcare delivery at hospitals.

Design/methodology/approach
Data were gathered using 170 patients in a cross-sectional survey with quantitative research methods at a public regional hospital. Results were obtained using descriptive analysis and regression analysis.

Findings
Generally, the study found that the health-related factors (the reception of staff, providers’ attitude, waiting time, competence and expertise and the hospital environment) that influence patients’ long-term relationship with the healthcare providers/hospital were statistically significant (p < 0.001). The findings showed that overall 90.0 per cent of the patients were very satisfied with the overall healthcare services at the hospital.

Research limitations/implications
Limited sample size, lack of examination of healthcare providers’ perspectives and non-application of qualitative methods make it difficult to give a true picture of how these can enhance patients’ intent to keep a long-term relationship with the healthcare providers/hospital.

Practical implications
The paper suggests that health policymakers and practitioners need to enhance measures that will make patients satisfied leading to their long-term commitment and cordial relationship with the healthcare providers/hospital.

Social implications
The study demonstrated how health-related factors will be associated with the patients’ agreement/intent to keep a long-term relationship with their service providers at hospitals. Thus, the overall hypothesis was true that there is a relationship between patients’ satisfaction with the healthcare experienced and their long-term relationship with healthcare providers/hospital.

Originality/value
This is one of the few studies conducted on the topic in the context of Ghana’s health sector. It recommends that there should be a good interpersonal relationship between healthcare providers and patients, as patients’ satisfaction is not based on only receiving treatment at the health facility.
Are attractive reviewers more persuasive? Examining the role of physical attractiveness in online reviews
Authors: Ozanne Marie , Liu Stephanie Q. , Mattila Anna S. 
Published: July 2019

PurposeWhile online reviews are of paramount importance in brand evaluations and purchase decisions, the impact of a reviewer’s attractiveness is not well understood. To bridge that gap, this paper aims to explore how physical attractiveness cues through profile photos influence customers’ brand evaluations.Design/methodology/approachThe first study assesses the impact of attractiveness and review valence on brand evaluations. The authors used an experimental design and tested the model with an ANCOVA. Study 2 examines the impact of attractiveness in the context of multiple reviews and tests attractiveness heuristic as the underlying mechanism.FindingsThe findings indicate that when an attractive (vs less-attractive) reviewer writes a positive review, brand evaluations are enhanced. However, such an effect does not occur with a negative review. With multiple reviews varying in valence, cognitive load activates the use of an attractiveness heuristic when a positive review is written by an attractive (vs less-attractive) reviewer, thus leading to enhanced brand evaluations.Originality/valueThese findings highlight the presence of the attractiveness halo effect in online reviews and offer important implications to social media marketers. While previous studies have largely focused on review characteristics (e.g. star ratings, strength of the argument, etc.), this study focuses on reviewer characteristics (i.e. attractiveness) and cognitive biases associated with online brand evaluations.
Highway to Hell? Managing expatriates in crisis
Authors: McNulty Yvonne , Lauring Jakob , Jonasson Charlotte , Selmer Jan 
Published: July 2019

PurposeThe purpose of this paper is to develop a conceptual framework of severe expatriate crises focusing on the occurrence of “fit-dependent” crisis events, which is when the crisis is “man made” and triggered by expatriates’ maladjustment or acculturation stress in the host country. The authors focus on the causes, prevention and management of fit-dependent expatriate crises.Design/methodology/approachThe authors develop a conceptual framework of fit-dependent expatriate crises that involves different levels of analysis.FindingsThe conceptual framework shows that crises can be triggered at micro, meso and macro levels ranging from the personal and family domains (micro), to the network and organisational domains (meso) as well as the host country domain (macro). The authors conceptualise these “domains of causes” as triggering maladjustment and acculturation stress that ultimately leads to a severe crisis event with correspondingly serious and potentially life-changing consequences. Furthermore, using a process perspective, the authors outline strategies for preventing and managing crises before, during and after the crisis occurs, discussing the support roles of various internal (organisational) and external (specialist) stakeholders.Originality/valueStudying the link between expatriation and crises is a highly relevant research endeavour because severe crisis events will impact on HRM policies, processes and procedures for dealing with employees living abroad, and will create additional challenges for HRM beyond what could normally be expected. Using attribution theory to explain why organisational support and intervention to assist expatriates during a crisis is not always forthcoming, and theories of social networks to elucidate the “first responder” roles of various support actors, the authors contribute to the expatriate literature by opening up the field to a better understanding of the dark side of expatriation that includes crisis definition, prevention, management and solutions.