bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of active Fel d1 from cats using an antiFel d1 egg IgY antibody
Authors: Satyaraj Ebenezer , Gardner Cari , Filipi Ivan , Cramer Kerry , Sherrill Scott 
Published: March 2019

AbstractBackgroundFel d1 is the most important allergen from cats. Fel d1 is produced primarily in saliva and spread to the haircoat during grooming and then transferred to the environment via hair and dander.ObjectivesA novel approach to reducing allergenic Fel d1 exposure was evaluated, involving binding the Fel d1 with an anti‐Fel d1 polyclonal egg IgY antibody. The hypothesis was that hair from cats who had been fed foods containing anti‐Fel d1 IgY would show a significant reduction in active Fel d1 (aFel d1).MethodsHair collected from 105 cats completing a 12‐week study was evaluated for aFel d1 via ELISA. Hair was collected four times over a 2‐week baseline period, then weekly during the 10 week treatment period during which cats consumed a food containing the anti‐Fel d1 IgY.ResultsBaseline aFel d1 (μg/g hair) varied greatly among the cats in this study. From week 3, there was a significant reduction in mean aFel d1 with an overall average decrease of 47% by week 10, ranging from a 33–71% decrease vs baseline. Cats with the highest baseline aFel d1 showed the greatest decrease in aFel d1.Conclusions & Clinical ImplicationsFeeding anti‐Fel d1 IgY to cats successfully reduced aFel d1 on their haircoat with the greatest decreases observed in cats with initially high levels. Feeding a diet with anti Fel d1 IgY significantly reduced the active Fel d1 on the hair of cats.
Long noncoding RNA LINC00961 inhibited cell proliferation and invasion through regulating the Wnt/β‐catenin signaling pathway in tongue squamous cell carcinoma
Authors: Zhang Lixia , Shao Linqin , Hu Ying 
Published: March 2019

AbstractTongue squamous cell carcinoma (TSCC) is the most frequent style of oral squamous cell carcinoma. However, the molecular mechanisms and function of LINC00961 in the TSCC progression remain unknown. In this study, we proved that LINC00961 expression was downregulated in TSCC cells (Tca8113, SCC1, SCC‐4, and SCC‐15) compared with normal tissue. In addition, we showed that LINC00961 expression was downregulated in TSCC samples compared with matched normal tissues. Moreover, ectopic expression of LINC00961 decreased TSCC cell growth and invasion and suppressed epithelial‐mesenchymal transition in TSCC cell. Furthermore, we indicated that overexpression of LINC00961 decreased β‐catenin expression. Knockdown of LINC00961 promoted cell proliferation and invasion partly via promoting the Wnt/β‐catenin signaling pathway. These results suggested that LINC00961 was downregulated in TSCC tissues and acted as a tumor suppressor gene in the development of TSCC.
Secreting oviduct epithelial cells of Coturnix coturnix japonica (QOEC) and changes to their proteome after nonviral transfection
Authors: Stadnicka Katarzyna , Dębowska Michalina , Dębski Janusz , Bajek Anna 
Published: March 2019

AbstractThe quail oviduct (Coturnix c. japonica) is a natural candidate avian bioreactor, while the secretive quail oviduct epithelial cells (QOECs) are potential in vitro producers of recombinant proteins and vaccines. In view of the need for highly performing and transformable cell lines, QOEC may potentially act as an alternative bioreactor platform to the existing ones, for example, to the Chinese hamster ovary. The aim of this work was to characterize QOECs and their response to nucleofection with a nonviral plasmid DNA carrying the human interferon‐α 2a gene (hIFNλ2a), in vitro. Primary QOEC cultures from laying quails (10‐15 weeks old) were characterized by their proliferation rate, doubling time, and multilineage differentiation. Electroporation to cell nuclei (nucleofection) was used to deliver nonviral plasmid DNA containing a reporter GFP and hIFN under the ovalbumin promoter. The posttransfection analysis included polymerase chain reaction, Western blot analysis, and liquid chromatography coupled to tandem mass spectrometry. QOEC showed a typical epithelial characteristic in a primary 2D monolayer culture system and retained secretive potential up to the first passage. QOEC showed differentiation into osteoblastic lineage after stimulation. The nucleofection mean efficiency was low (2.3%). Differences of up to 10% in the proteomic profiles between nontransfected and transfected QOEC were found, the most important of these were related to the absence of keratins and cell‐adhesion proteins in the transfected QOEC. Concluding, with the practical information provided here, QOEC have the potential to serve as an avian secreting cellular platform. QOEC may be further transformed to cell lineage to meet the requirement for a stable, electrocompetent, and transfectable model. The first proteomic comparison of QOEC delivered in this study showed, in the majority, a stable proteome of the nontransfected vs transfected QOEC.
Comparison of osteogenic differentiation potential of induced pluripotent stem cells on 2D and 3D polyvinylidene fluoride scaffolds
Authors: Mirzaei Ali , Moghadam Abbas Shapouri , Abazari Mohamad Foad , Nejati Fatemeh , Torabinejad Sepehr , Kaabi Mohamad , Enderami Seyed Ehsan , Ardeshirylajimi Abdolreza , Darvish Maryam , Soleimanifar Fatemeh , Saburi Ehsan 
Published: March 2019

AbstractIn recent decades, tissue engineering has been the most contributor for introducing 2D and 3D biocompatible osteoinductive scaffolds as bone implants. Polyvinylidene fluoride (PVDF), due to the unique mechanical strength and piezoelectric properties, can be a good choice for making a bone bioimplant. In the present study, PVDF nanofibers and film were fabricated as 3D and 2D scaffolds, and then, osteogenic differentiation potential of the human induced pluripotent stem cells (iPSCs) was investigated when grown on the scaffolds by evaluating the common osteogenic markers in comparison with tissue culture plate. Biocompatibility of the fabricated scaffolds was confirmed qualitatively and quantitatively by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and scanning electron microscopy assays. Human iPSCs cultured on PVDF nanofibers showed a significantly higher alkaline phosphate activity and calcium content compared with the iPSCs cultured on PVDF film. Osteogenic‐related genes and proteins were also expressed in the iPSCs seeded on PVDF nanofibers significantly higher than iPSCs seeded on PVDF film, when investigated by real‐time reverse transcription polymerase chain reaction and western blot analysis, respectively. According to the results, the PVDF nanofibrous scaffold showed a greater osteoinductive property compared with the PVDF film and due to the material similarity of the scaffolds, it could be concluded that the 3D structure could lead to better bone differentiation. Taken together, the obtained results demonstrated that human iPSC‐seeded PVDF nanofibrous scaffold could be considered as a promising candidate for use in bone tissue engineering applications.
Conditioned media from adipocytes promote proliferation, migration, and invasion in melanoma and colorectal cancer cells
Authors: Ko Jeong‐Hyeon , Um Jae‐Young , Lee Seok‐Geun , Yang Woong Mo , Sethi Gautam , Ahn Kwang Seok 
Published: March 2019

AbstractEpidemiological evidence suggests that obesity can significantly increase the risk of various cancers, although the mechanisms underlying this link are completely unknown. Here, we analyzed the effect of adipocytes on melanoma and colon cancer cells proliferation, migration, and invasion. The potential effects of conditioned media (CM) obtained from differentiated mouse 3T3‐L1 cells and human adipose tissue‐derived mesenchymal stem cells (hAMSC) on the proliferation, migration, and invasion of B16BL6 melanoma and colon 26‐L5 cancer cells were investigated. The 3T3‐L1 and hAMSC CM increased cell proliferation, migration, and invasion in both the cell lines. In addition, adipocytes CM increased matrix metalloproteinase 9 (MMP‐9) and MMP‐2 activity in both B16BL6 and colon 26‐L5 cells. These effects were found to be associated with an increased expression of various oncogenic proteins in B16BL6 and colon 26‐L5 cells. Also, adipocyte CM induced Akt and mTOR activation in both tumor cell lines, and the pharmacological inhibition of Akt and mTOR blocked the CM induced Akt as well as mTOR activation and CM‐stimulated melanoma and colon cancer cell proliferation, migration, and invasion. These data suggest that adipocyte promotes melanoma and colon cancer progression through modulating the expression of diverse proteins associated with cancer growth and metastasis as well as modulation of the Akt/mTOR signaling.
Efficiency of two constructed wetland systems for wastewater treatment: removal of bacterial indicators and enteric viruses
Authors: Kaliakatsos Andreas , Kalogerakis Nicolas , Manios Thrassyvoulos , Venieri Danae 
Published: March 2019

AbstractBACKGROUNDPathogenic microorganisms at the effluents of treatment plants entail a potential risk to public health. Enteric viruses such as adenoviruses (AdVs) and enteroviruses (EVs) are responsible for many waterborne diseases. Wastewater treatment methods eliminate successfully the load of indicators but other pathogens such as viruses have been detected in high concentrations at the effluents of wastewater plants. In this perspective, two constructed wetlands (CWs) systems (S1 and S2) were employed and fed with primary wastewater. S1 consists of a free water surface (FWS) wetland and a horizontal subsurface flow (HSSF) wetland working in series, while S2 consists only of the HSSF. Also, both systems included a sand filter (SF) after the HSSF.RESULTSResults showed that these low‐cost systems are capable of eliminating effectively the bacterial indictors (total coliforms, Escherichia coli, Enterococci) achieving removal rates of almost 99% for S1 (3.2–4 Log units) and 89–98% for S2 (1.9–2.7 Log units). Regarding viruses, AdVs, EVs and phages were detected at all sampling points and during all seasons, and they were only partly removed in S1 and/or S2. For example, the virus load was decreased by 2.5 Log units for AdVs and 3.4 Log units for EVs in the case of S1, while the respective values were 4.3 and 1.9 Log units for S2.CONCLUSIONSGenerally, CWs eliminated successfully the bacterial load, while the remaining virus load was significant, thus strengthening the argument that viruses could be a reliable indicator for fecal contamination. © 2019 Society of Chemical Industry
Organic/inorganic nanohybrids as multifunctional gene delivery systems
Authors: Guo Kangli , Zhao Xiaoyi , Dai Xiaoguang , Zhao Nana , Xu Fu‐Jian 
Published: March 2019

AbstractIn this review, we summarize the rational design and versatile application of organic/inorganic hybrid gene carriers as multifunctional delivery systems. Organic/inorganic nanohybrids with both organic and inorganic components in one nanoparticle have attracted intense attention because of their favorable properties. Particularly, nanohybrids comprising cationic polymers and inorganic nanoparticles are considered to be promising candidates as multifunctional gene delivery systems. In this review, we begin with an introduction of gene delivery and gene carriers to demonstrate the incentive for fabricating nanohybrids as multifunctional carriers. Next, the construction strategies and morphology effects of organic/inorganic hybrid gene carriers are summarized and discussed. Both sections provide valuable information for the design and synthesis of hybrid gene carriers with superior properties. Finally, an overview is provided of the application of nanohybrids as multifunctional gene carriers. Diverse therapies and versatile imaging‐guided therapies have been achieved via the rational design of nanohybrids. In addition to a simple combination of the functions of organic and inorganic components, the performances arising from the synergistic effects of both components are considered to be more intriguing. In summary, this review might offer guidance for the understanding of organic/inorganic nanohybrids as multifunctional gene delivery systems.
Novel insights into the clinical and molecular spectrum of congenital disorders of autophagy
Authors: Teinert Julian , Behne Robert , Wimmer Miriam , Ebrahimi‐Fakhari Darius 
Published: March 2019

AbstractAutophagy is a fundamental and conserved catabolic pathway that mediates the degradation of macromolecules and organelles in lysosomes. Autophagy is particularly important to postmitotic and metabolically active cells such as neurons. The complex architecture of neurons and their long axons pose additional challenges for efficient recycling of cargo. Not surprisingly autophagy is required for normal central nervous system development and function. Several single‐gene disorders of the autophagy pathway have been discovered in recent years giving rise to a novel group of inborn errors of metabolism referred to as congenital disorders of autophagy. While these disorders are heterogeneous, they share several clinical and molecular characteristics including a prominent and progressive involvement of the central nervous system leading to brain malformations, developmental delay, intellectual disability, epilepsy, movement disorders, and cognitive decline. On brain magnetic resonance imaging a predominant involvement of the corpus callosum, the corticospinal tracts and the cerebellum are noted. A storage disease phenotype is present in some diseases, underscoring both clinical and molecular overlaps to lysosomal storage diseases. This review provides an update on the clinical, imaging, and genetic spectrum of congenital disorders of autophagy and highlights the importance of this pathway for neurometabolism and childhood‐onset neurological diseases.
A signal amplification system on a lateral flow immunoassay detecting for hepatitis e‐antigen in human blood samples
Authors: Si Jinhong , Li Jinfeng , Zhang Ling , Zhang Weiyun , Yao Jinxiu , Li Tingting , Wang Wenjing , Zhu Weihang , Allain Jean‐Pierre , Fu Yongshui , Li Chengyao 
Published: March 2019

AbstractHepatitis B e‐antigen (HBeAg) is the secretory form of the nucleocapsid of the hepatitis B virus (HBV), which is a marker of viral replication. In this study, a novel signal amplification system (SAS) based on the lateral flow immunoassay (LFIA) was used for rapid detection of HBeAg in blood samples from patients or blood donors. In this assay, the detection antibody was conjugated with gold nanoparticles (GNPs), and the capture antibody was labeled with biotin. The presence of targeting antigen HBeAg in blood sample would act as a bridge with biotinylated captured antibody and GNP‐conjugated detection antibody to form the dendritic nanoparticle complex. The dendritic complexes in the sample solution were migrated and immobilized on the testing line of strip coated with antibiotin antibodies. Signal intensity was massively amplified by the SAS, which was positively correlated with the concentration of targeting antigen in the blood sample and was assessed by eyes or strip scanner. The SAS worked only when targeting antigens were present in the sample. By using this SAS‐LFIA, we were able to detect a very low concentration of HBeAg (9 ng/mL), which was 27‐fold sensitive than that by conventional LFIA (cLFIA). A number of 420 blood samples were detested by this novel SAS‐LFIA, the results were in accordance with those of enzyme‐linked immunosorbent assay (ELISA) completely, while the cLFIA missed an HBeAg‐positive sample. In conclusion, the novel SAS has high specificity and sensitivity, which can be used to replace the conventional rapid test and ELISA in clinical diagnosis.
Spillover and crossover of work resources: A test of the positive flow of resources through work–family enrichment
Authors: Carlson Dawn S. , Thompson Merideth J. , Crawford Wayne S. , Kacmar K. Michele 
Published: March 2019

SummaryIn their seminal model of work–family enrichment, Greenhaus and Powell (2006) theorized five categories of work resources to have an impact on work–family enrichment. Using a matched set of 351 dual‐career spouses, we explored how these unique work resources contribute to work–family enrichment and how they spill over to shape job incumbents' family outcomes and cross over to spouse outcomes. Results support indirect effects for the work resources of developmental experiences, supervisor support, schedule control, and self‐efficacy, but not salary, on marital satisfaction and family functioning through the job incumbent's work–family enrichment. In addition, we found support for the crossover of these indirect effects to the spouse through the spouse's positive crossover transmission, which facilitated the spouse's marital satisfaction and commitment to the job incumbent's organization. Our results suggest that multiple work resources have an impact on work–family enrichment and that the flow of positive work resources through work–family enrichment spills over to impact outcomes for the job incumbent and crosses over to the spouse.
Correlation between MTHFR polymorphisms and glaucoma: A meta‐analysis
Authors: Zhang Ling , Chen Bin 
Published: March 2019

AbstractBackgroundWhether methylenetetrahydrofolate reductase (MTHFR) polymorphisms are implicated in glaucoma remains controversial. Therefore, we performed this study to better assess the relationship between MTHFR polymorphisms and the likelihood of glaucoma.MethodsA systematic research of PubMed, Medline, and Embase was performed to retrieve relevant articles. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated.ResultsA total of 18 studies with 7,168 participants were analyzed. In overall analyses, a significant association with the likelihood of glaucoma was detected for the rs1801133 polymorphism in dominant (p = 0.04, OR = 0.90, 95%CI 0.81–1.00) and allele (p = 0.02, OR = 0.91, 95%CI 0.84–0.98) comparisons. Further, subgroup analyses by ethnicity revealed that both rs1801131 and rs1801133 polymorphisms were significantly associated with the likelihood of glaucoma in West Asians. However, no positive results were detected for two investigated polymorphisms in East Asians and Caucasians.ConclusionOur findings indicated that rs1801131 and rs1801133 polymorphisms may serve as genetic biomarkers of glaucoma in West Asians.
Preparation of magnetite and silver poly(2‐acrylamido‐2‐methyl propane sulfonic acid‐co‐acrylamide) nanocomposites for adsorption and catalytic degradation of methylene blue water pollutant
Authors: Atta Ayman M , Gafer Amany K , Al‐Lohedan Hamad A , Abdullah Mahmood MS , Ezzat Abdelrahman O 
Published: March 2019

AbstractThe aim of the present work was to prepare microgel nanocomposites based on silver and magnetite to apply as adsorbents and heterogeneous catalysts for removal of methylene blue (MB) cationic dye from aqueous solution. For this, 2‐acrylamido‐2‐methylpropane sulfonic acid (AMPS) and acrylamide (AAm) monomers were used to prepare AMPS/AAm microgel based on the emulsion technique. Ag and Fe3O4 nanoparticles were embedded into the AMPS/AAm microgel using the in situ technique. Their particle sizes, surface charges, crystalline lattice structure, morphology, magnetic properties and thermal stability were investigated. The AMPS/AAm hydrogel nanocomposites were used as an adsorbent to remove MB dye. The AMPS/AAm microgel nanocomposites were tested as catalysts to reduce MB and degrade its chemical structure with heterogeneous Fenton oxidation using Ag and Fe3O4 nanocomposites, respectively. This study presents promising data as the prepared materials used as adsorbents and catalysts show competitive features compared with the data presented in the literature. © 2019 Society of Chemical Industry
Novel porous organic triazine‐based polyimide with high nitrogen levels for highly efficient removal of Ni(II) from aqueous solution
Authors: Mokhtari Nazanin , Dinari Mohammad , Rahmanian Omid 
Published: March 2019

AbstractIn this study, a porous organic triazine‐based polyimide (PPI network) was prepared from 2,4,6‐tris(hydrazino)‐s‐triazine and 3,4,9,10‐perylenetetracarboxylic dianhydride. TGA, Fourier transform infrared spectroscopy, field emission SEM, XRD and a nitrogen sorption study confirmed the PPI network structure. Then, the synthesized PPI network was used to evaluate Ni2+ ion removal from aqueous solution and the effective parameters on adsorption functions of Ni2+ ions such as initial concentration, contact time and pH of the solution in batch adsorption experiments was studied. The results showed that the maximum adsorption capacity (qm) of Ni2+ ions was 36.1 mg g−1 in only 30 min with a pH of 7. The kinetics and adsorption isotherm were identified to be better fitted by the pseudo‐second‐order model and the Langmuir model, respectively. Based on the results, the proposed adsorbent has good potential for removing Ni2+ ions from aqueous solutions. © 2019 Society of Chemical Industry
Optimized peptide functionalization of thiol‐acrylate emulsion‐templated porous polymers leads to expansion of human pluripotent stem cells in 3D culture
Authors: Ratcliffe Jessie L. , Walker Marc , Eissa Ahmed M. , Du Shengrong , Przyborski Stefan A. , Laslett Andrew L. , Cameron Neil R. 
Published: March 2019

ABSTRACTHighly porous polymers produced by polymerization of the continuous phase of a high internal phase emulsion have been developed as scaffolds for 3D culture of human pluripotent stem cells. These emulsion‐templated polymerized high internal phase emulsion (polyHIPE) materials have an interconnected network of pores that provide support for the cells, while also allowing both cell ingress and nutrient diffusion. Thiol‐acrylate polyHIPE materials were prepared by photopolymerization, which, due to a competing acrylate homopolymerization process, leads to a material with residual surface thiols. These thiols were then used as a handle to allow postpolymerization functionalization with both maleimide and a maleimide‐derivatized cyclo‐RGDfK peptide, via Michael addition under benign conditions. Functionalization was evaluated using an Ellman's colorimetric assay, to monitor the residual thiol concentration, and X‐ray photoelectron spectroscopy. Maleimide was used as a model molecule to optimize conditions prior to peptide‐functionalization. The use of triethylamine as a catalyst and a mixed ethanol‐aqueous solvent system led to optimized reaction between surface‐bound thiols and maleimide. Peptide‐functionalized materials showed improved attachment and infiltration of human pluripotent stem cells over 7 days, demonstrating their promise as a scaffold for 3D stem cell culture and expansion. © 2019 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2019, 57, 1974–1981