bluuebunny/crossref_metadata_2025_split huggingface dataset:
SNF2L maintains glutathione homeostasis by initiating SLC7A11 transcription through chromatin remodeling
Authors: Zhang Jiaguan , Gao Zeshou , Yang Yi , Li Zhenhao , Wu Binjie , Fan Chunxin , Zheng Yuyan , Yang Ruohan , Zhang Fangrong , Lin Xiaohuang , Zheng Daoshan 
Published: November 2024

AbstractSNF2L encodes an ISWI chromatin remodeling factor that promotes gene transcription and is consistently elevated in cancers. Previous studies have shown that inhibiting SNF2L expression in cancer cells leads to significant growth suppression, DNA damage, and cell death. However, the underlying mechanisms remain poorly understood. In this study, we demonstrated that cancer cells lacking SNF2L show significantly decreased glutathione (GSH) levels, leading to elevated reactive oxygen species (ROS) and increased oxidative stress. SNF2L deficiency also heightened the sensitivity of cancer cells to APR-246, a drug that depletes GSH and induces oxidative stress, consequently decreasing cell viability and increasing ROS levels, regardless of p53 status. Mechanistically, we found that NRF2 recruits SNF2L to the SLC7A11 promoter, leading to increased chromatin accessibility and facilitating SLC7A11 transcription. This results in decreased cystine uptake and impaired GSH biosynthesis. These findings suggest that targeting the SNF2L/SLC7A11 axis could enhance the effectiveness of APR-246 by depleting GSH and increasing ROS level in cancer cells, highlighting SNF2L as a promising therapeutic target.
Triggering the 2022 eruption of Mauna Loa
Authors: Lynn Kendra J. , Downs Drew T. , Trusdell Frank A. , Wieser Penny E. , Rangel Berenise , McDade Baylee , Hotovec-Ellis Alicia J. , Bennington Ninfa , Anderson Kyle R. , Ruth Dawn C. S. , DeVitre Charlotte L. , Ellis Andria P. , Nadeau Patricia A. , Clor Laura , Kelly Peter , Dotray Peter J. , Chang Jefferson C. 
Published: November 2024

Abstract
Distinguishing periods of intermittent unrest from the run-up to eruption is a major challenge at volcanoes around the globe. Comparing multidisciplinary monitoring data with mineral chemistry that records the physical and spatio-temporal evolution of magmas fundamentally advances our ability to forecast eruptions. The recent eruption of Mauna Loa, Earth’s largest active volcano, provides a unique opportunity to differentiate unrest from run-up and improve forecasting of future eruptions. After decades of intermittent seismic and geodetic activity over 38 years of repose, Mauna Loa began erupting on 27 November 2022. Here we present a multidisciplinary synthesis that tracks the spatio-temporal evolution of precursory activity by integrating mineral and melt chemistry, fluid inclusion barometry, numerical modeling of mineral zoning, syn-eruptive gas plume measurements, the distribution and frequency of earthquake hypocenters, seismic velocity changes, and ground deformation. These diverse data indicate that the eruption occurred following a 2-month period of sustained magma intrusion from depths of 3–5 km up to 1–2 km beneath the summit caldera, providing a new model of the plumbing system at this very high threat volcano. Careful correlation of both the geochemistry and instrumental monitoring data improves our ability to distinguish unrest from the run-up to eruption by providing deeper understanding of the both the monitoring data and the magmatic system—an approach that could be applied at other volcanic systems worldwide.
Neutralizing antibody correlate of protection against severe-critical COVID-19 in the ENSEMBLE single-dose Ad26.COV2.S vaccine efficacy trial
Authors: Carpp Lindsay N. , Hyrien Ollivier , Fong Youyi , Benkeser David , Roels Sanne , Stieh Daniel J. , Van Dromme Ilse , Van Roey Griet A. , Kenny Avi , Huang Ying , Carone Marco , McDermott Adrian B. , Houchens Christopher R. , Martins Karen , Jayashankar Lakshmi , Castellino Flora , Amoa-Awua Obrimpong , Basappa Manjula , Flach Britta , Lin Bob C. , Moore Christopher , Naisan Mursal , Naqvi Muhammed , Narpala Sandeep , O’Connell Sarah , Mueller Allen , Serebryannyy Leo , Castro Mike , Wang Jennifer , Petropoulos Christos J. , Luedtke Alex , Lu Yiwen , Yu Chenchen , Juraska Michal , Hejazi Nima S. , Wolfe Daniel N. , Sadoff Jerald , Gray Glenda E. , Grinsztejn Beatriz , Goepfert Paul A. , Bekker Linda-Gail , Gaur Aditya H. , Veloso Valdilea G. , Randhawa April K. , Andrasik Michele P. , Hendriks Jenny , Truyers Carla , Vandebosch An , Struyf Frank , Schuitemaker Hanneke , Douoguih Macaya , Kublin James G. , Corey Lawrence , Neuzil Kathleen M. , Follmann Dean , Koup Richard A. , Donis Ruben O. , Gilbert Peter B. ,   On behalf of the Immune Assays Team,   the Coronavirus Vaccine Prevention Network (CoVPN)/ENSEMBLE Team, Van Dromme Ilse , Van Roey Griet A. ,   the United States Government (USG)/CoVPN Biostatistics Team
Published: November 2024

AbstractAssessment of immune correlates of severe COVID-19 has been hampered by the low numbers of severe cases in COVID-19 vaccine efficacy (VE) trials. We assess neutralizing and binding antibody levels at 4 weeks post-Ad26.COV2.S vaccination as correlates of risk and of protection against severe-critical COVID-19 through 220 days post-vaccination in the ENSEMBLE trial (NCT04505722), constituting ~4.5 months longer follow-up than our previous correlates analysis and enabling inclusion of 42 severe-critical vaccine-breakthrough cases. Neutralizing antibody titer is a strong inverse correlate of severe-critical COVID-19, with estimated hazard ratio (HR) per 10-fold increase 0.35 (95% CI: 0.13, 0.90). In a multivariable model, HRs are 0.31 (0.11, 0.89) for neutralizing antibody titer and 1.22 (0.49, 3.02) for anti-Spike binding antibody concentration. VE against severe-critical COVID-19 rises with neutralizing antibody titer: 63.1% (95% CI: 40.0%, 77.3%) at unquantifiable [<4.8975 International Units (IU)50/ml], 85.2% (47.2%, 95.3%) at just-quantifiable (5.2 IU50/ml), and 95.1% (81.1%, 96.9%) at 90th percentile (30.2 IU50/ml). At the same titers, VE against moderate COVID-19 is 32.5% (11.8%, 48.4%), 33.9% (19.1%, 59.3%), and 60.7% (40.4%, 76.4%). Protection against moderate vs. severe disease may require higher antibody levels, and very low antibody levels and/or other immune responses may associate with protection against severe disease.
Label-free biosensor assay decodes the dynamics of Toll-like receptor signaling
Authors: Holze Janine , Lauber Felicitas , Soler Sofía , Kostenis Evi , Weindl Günther 
Published: November 2024

AbstractThe discovery of Toll-like receptors (TLRs) represented a significant breakthrough that paved the way for the study of host-pathogen interactions in innate immunity. However, there are still major gaps in understanding TLR function, especially regarding the early dynamics of downstream TLR pathways. Here, we present a label-free optical biosensor-based assay as a method for detecting TLR activation in a native and label-free environment and defining the dynamics of TLR pathway activation. This technology is sufficiently sensitive to detect TLR signaling and readily discriminates between different TLR signaling pathways. We define pharmacological modulators of cell surface and endosomal TLRs and downstream signaling molecules and uncover TLR signaling signatures, including potential biased receptor signaling. These findings highlight that optical biosensor assays complement traditional assays that use a single endpoint and have the potential to facilitate the future design of selective drugs targeting TLRs and their downstream effector cascades.
Hepatic SerpinA1 improves energy and glucose metabolism through regulation of preadipocyte proliferation and UCP1 expression
Authors: Okagawa Shota , Sakaguchi Masaji , Okubo Yuma , Takekuma Yuri , Igata Motoyuki , Kondo Tatsuya , Takeda Naoki , Araki Kimi , Brandao Bruna Brasil , Qian Wei-Jun , Tseng Yu-Hua , Kulkarni Rohit N. , Kubota Naoto , Kahn C. Ronald , Araki Eiichi 
Published: November 2024

AbstractLipodystrophy and obesity are associated with insulin resistance and metabolic syndrome accompanied by fat tissue dysregulation. Here, we show that serine protease inhibitor A1 (SerpinA1) expression in the liver is increased during recovery from lipodystrophy caused by the adipocyte-specific loss of insulin signaling in mice. SerpinA1 induces the proliferation of white and brown preadipocytes and increases the expression of uncoupling protein 1 (UCP1) to promote mitochondrial activation in mature white and brown adipocytes. Liver-specific SerpinA1 transgenic mice exhibit increased browning of adipose tissues, leading to increased energy expenditure, reduced adiposity and improved glucose tolerance. Conversely, SerpinA1 knockout mice exhibit decreased adipocyte mitochondrial function, impaired thermogenesis, obesity, and systemic insulin resistance. SerpinA1 forms a complex with the Eph receptor B2 and regulates its downstream signaling in adipocytes. These results demonstrate that SerpinA1 is an important hepatokine that improves obesity, energy expenditure and glucose metabolism by promoting preadipocyte proliferation and activating mitochondrial UCP1 expression in adipocytes.
Antithrombotic coating with sheltered positive charges prevents contact activation by controlling factor XII–biointerface binding
Authors: Ji Haifeng , Yu Kai , Abbina Srinivas , Xu Lin , Xu Tao , Cheng Shengjun , Vappala Sreeparna , Arefi S. M. Amin , Rana Md Mohosin , Chafeeva Irina , Drayton Matthew , Gonzalez Kevin , Liu Yun , Grecov Dana , Conway Edward M. , Zhao Weifeng , Zhao Changsheng , Kizhakkedathu Jayachandran N. 
Published: November 2024

AbstractAntithrombotic surfaces that prevent coagulation activation without interfering with haemostasis are required for blood-contacting devices. Such materials would restrain device-induced thrombogenesis and decrease the need for anticoagulant use, thereby reducing unwanted bleeding. Here, by optimizing the interactions with coagulation factor XII rather than preventing its surface adsorption, we develop a substrate-independent antithrombotic polymeric coating with sheltered positive charges. The antithrombic properties of the coating were demonstrated in vitro with human blood and in vivo using a carotid artery–jugular vein shunt model in rabbits. The coating exhibits a strong interaction with factor XII, but results in a low reciprocal activation of the contact pathway that triggers clot formation. These findings contradict the prevailing strategy of designing antithrombotic materials through protein-repelling surfaces. Overall, the polymeric coating we describe can benefit most blood-contacting devices and is a useful engineering guideline for designing surfaces with improved antithrombotic properties.
Analysis of behavioral flow resolves latent phenotypes
Authors: von Ziegler Lukas M. , Roessler Fabienne K. , Sturman Oliver , Waag Rebecca , Privitera Mattia , Duss Sian N. , O’Connor Eoin C. , Bohacek Johannes 
Published: November 2024

AbstractThe accurate detection and quantification of rodent behavior forms a cornerstone of basic biomedical research. Current data-driven approaches, which segment free exploratory behavior into clusters, suffer from low statistical power due to multiple testing, exhibit poor transferability across experiments and fail to exploit the rich behavioral profiles of individual animals. Here we introduce a pipeline to capture each animal’s behavioral flow, yielding a single metric based on all observed transitions between clusters. By stabilizing these clusters through machine learning, we ensure data transferability, while dimensionality reduction techniques facilitate detailed analysis of individual animals. We provide a large dataset of 771 behavior recordings of freely moving mice—including stress exposures, pharmacological and brain circuit interventions—to identify hidden treatment effects, reveal subtle variations on the level of individual animals and detect brain processes underlying specific interventions. Our pipeline, compatible with popular clustering methods, substantially enhances statistical power and enables predictions of an animal’s future behavior.
Spatially integrated cortico-subcortical tracing data for analyses of rodent brain topographical organization
Authors: Øvsthus Martin , van Swieten Maaike M. H. , Puchades Maja A. , Tocco Chiara , Studer Michèle , Bjaalie Jan G. , Leergaard Trygve B. 
Published: November 2024

AbstractThe cerebral cortex extends axonal projections to several subcortical brain regions, including the striatum, thalamus, superior colliculus, and pontine nuclei. Experimental tract-tracing studies have shown that these subcortical projections are topographically organized, reflecting the spatial organization of sensory surfaces and body parts. Several public collections of mouse- and rat- brain tract-tracing data are available, with the Allen mouse brain connectivity atlas being most prominent. There, a large body of image data can be inspected, but it is difficult to combine data from different experiments and compare spatial distribution patterns. To enable co-visualization and comparison of topographical organization in mouse brain cortico-subcortical projections across experiments, we represent axonal labelling data as point data in a common 3D brain atlas space. We here present a collection of point-cloud data representing spatial distribution of corticostriatal, corticothalamic, corticotectal, and corticopontine projections in mice and exemplify how these spatially integrated point data can be used as references for experimental investigations of topographic organization in transgenic mice, and for cross-species comparison with corticopontine projections in rats.
High-resolution topography reveals morphological changes of Stromboli volcano following the July 2024 eruption
Authors: Civico Riccardo , Ricci Tullio , Cecili Alessandro , Scarlato Piergiorgio 
Published: November 2024

AbstractThe July 2024 eruption of Stromboli volcano has been characterised by the manifestation, at variable intensity, of the entire repertoire of volcanic events that Stromboli volcano is capable of, and is by far the one that has most changed the morphology of the crater terrace and of the Sciara del Fuoco slope in the last decades. We present the results of an Unoccupied Aircraft System (UAS) survey performed on 14 July 2024 and consisting of 4,988 visible and thermal photographs coupled with Structure-from-Motion photogrammetry that allowed us to produce a high-resolution (0.2 m/pixel) Digital Surface Model (DSM). We documented the profound morphological changes of the Stromboli volcano resulting from the 4–11 July 2024 eruption and obtained elevation and volume change estimates by differencing our survey and a UAS-derived pre-event surface (24 May 2024).
A novel pillar-layered MOF with urea linkers as a capable catalyst for synthesis of new 1,8-naphthyridines via the anomeric-based oxidation
Authors: Beiranvand Masoumeh , Habibi Davood , Khodakarami Hosein 
Published: November 2024

AbstractMetal-based catalysts play an essential role in organic chemistry and the chemical industry. This research designed and successfully synthesized a pillar-layered metal–organic framework (MOF) with the urea linkers, namely Basu-HDI, as a novel and efficient heterogeneous catalyst. Various techniques such as FT-IR, EDX, elemental mapping, SEM, XRD, BET, and TGA/DTA studied its structure and morphology. Then, we investigated the synthesis of new 1,8-naphthyridines utilizing Basu-HDI in mild conditions via a one‐pot, three‐component tandem Knoevenagel/Michael/ cyclization/anomeric-based oxidation reaction. Final products were achieved by anomeric-based oxidation without employing an oxidation agent. Remarkably, this tandem process gave a good range of new 1,8-naphthyridines with high yields in a short reaction time. The pure products were confirmed by FT-IR, 1H NMR, 13C NMR, and mass spectrometry techniques. Moreover, the introduced catalyst showed good efficiency and stability and can be reused four times without significantly reducing efficiency.
Exposure to constant artificial light alters honey bee sleep rhythms and disrupts sleep
Authors: Kim Ashley Y. , Velazquez Aura , Saavedra Belen , Smarr Benjamin , Nieh James C. 
Published: November 2024

AbstractArtificial light at night (ALAN) changes animal behavior in multiple invertebrates and vertebrates and can result in decreased fitness. However, ALAN effects have not been studied in European honey bees (Apis mellifera), an important pollinator in which foragers show strong circadian rhythmicity. Colonies can be exposed to ALAN in swarm clusters, when bees cluster outside the nest on hot days and evenings, and, in limited cases, when they build nests in the open. We captured and maintained foragers in incubated cages and subjected them to constant light (LL), constant dark (DD), or 12 h light:12 h dark (LD) cycle, and observed them with infrared cameras. After 79 h, there was a significant interaction of treatment and time because LL bees slept less. In detail, the bees maintained a regular sleep pattern for three days but LL bees showed a shift on the fourth day. LL bees had the largest sleep differences from LD controls, with trends of lengthened periods and increased phase misalignment from both LD and DD bees. LL bees also experienced significantly more disturbances from their nestmates and preferred to sleep in the lower portion of the cages, which had significantly lower light intensity. These findings suggest that ALAN can disrupt the sleep of honey bee foragers, which has implications for their behavior and overall colony health.
Thermal and flow dynamics of an inclined air heat exchanger equipped with spring turbulators in the transition flow regime
Authors: Vishwakarma Devendra Kumar , Bhattacharyya Suvanjan , Soni Manoj K. 
Published: November 2024

AbstractThe research involves an experimental investigation into the performance of a flow assisting air heat exchanger under varying angular orientation and uniform external heat fluxes without and with spring turbulators. The investigation was performed for Reynolds numbers ranging from 511 to 9676 and inclination angle 15° and 30°. Three heat fluxes (2, 3, and 4 kW/m2) were applied to the test section to investigate the effect of external surface heating on the range of transition flow regime and thermohydraulic performance. Transition from laminar to turbulent flow for plain channel at different heat fluxes and inclinations occurs within specific Reynolds number ranges: 2436–4446 for 15° inclination at 4 kW/m2, 2574–4289 at 3 kW/m2, and 2850–4152 at 2 kW/m2; for 30° inclination, the ranges are 2518–4151, 2712–4361, and 2992–4346 at the respective heat fluxes. When it comes to the effect of inclination on Nusselt number, the transition occurs sooner at lower angles, but is delayed as the angle increases. Additionally, the Nusselt number decreases as the angle of inclination increases. When comparing the Nusselt numbers of plain tubes to those with spring turbulators, the latter shows a significantly greater enhancement. In laminar flow, a maximum 100% deviation exists between highest and lowest friction factors, decreasing to 75% with increasing Reynolds number; all insert configurations exhibit highest friction factor at 15° due to stronger buoyancy forces.
Time-varying trends from Arctic ozonesonde time series in the years 1994–2022
Authors: Nilsen K. , Kivi R. , Laine M. , Poyraz D. , Van Malderen R. , von der Gathen P. , Tarasick D. W. , Thölix L. , Jepsen N. 
Published: November 2024

AbstractAlthough evidence of recovery in Antarctic stratospheric ozone has been found, evidence of recovery in Arctic ozone is still elusive, even though 25 years have passed since the peak in ozone depleting substances. Here we have used a Dynamic Linear Model to derive time-varying trends over 20-year periods in the Arctic ozone time series, measured in-situ by ozonesondes from 6 stations, from 1994 to 2022. The model accounts for seasonality, external forcing and 1st-order correlation in the residuals. As proxies for the external forcing, we have used tropopause pressure (replaced with Arctic Oscillation in the troposphere), eddy heat flux, the volume of polar stratospheric clouds multiplied by effective equivalent stratospheric chlorine, and solar radio flux at 10.7 cm for the 11-year solar cycle. Our results indicate that the ozone recovery in the lower Arctic stratosphere is not detectable. Though significant positive trends have been detected prior to 2017 at some stations, there are no statistically significant positive trends after 2017. Moreover, at a number of stations the trends after 2019 are rather negative and significant, varying between − 0.30 ± 0.25 and − 1.00 ± 0.85% per decade. Furthermore, the Arctic troposphere exhibited only statistically significant negative trends over 20-year periods ending in 2017 or later, varying between − 0.31 ± 0.27 and − 1.76 ± 0.41% per decade. These results highlight the importance of continued monitoring of the Arctic ozone.
Design, analysis, and demonstration of the COAST guidewire robot with middle tube rotation for endovascular interventions
Authors: Ravigopal Sharan R. , Konda Revanth , Malhotra Nidhi , Desai Jaydev P. 
Published: November 2024

AbstractMinimally invasive procedures for endovascular interventions involve manual navigation of a guidewire. Endovascular interventions encompassing highly tortuous vessels would benefit from guidewires which exhibit higher dexterity. This paper introduces a version of the COAST (COaxially Aligned STeerable) guidewire system capable of exhibiting higher dexterity. The system presented in this paper consists of three coaxially aligned tubes with a tendon to actuate the middle tube. Furthermore, it is possible to independently rotate the middle tube with respect to the outer tube. This variation enables the guidewire to achieve curvature in different planes while avoiding rotation of the entire structure. We also present the simulated stability of the guidewire with different outer tube geometries and experimentally validate the model. Experimental analysis and modeling of the kinematic behavior of the system is presented. A model to calculate the curvature vs. tendon stroke relationship for the optimal notch geometry is presented with an average RMSE of 0.16 mm. A control strategy addressing the snapping instabilities to ensure reliable operation is discussed. A custom phantom vessel and an aortic arch phantom model were used to demonstrate the ability of the system to safely navigate through tortuous pathways without exhibiting these elastic instabilities.