bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiplex ligation dependent probe amplification - A useful, fast and cost-effective method for identification of small supernumerary marker chromosome in children with developmental delay and congenital heart defect
Authors: Crauciuc George Andrei , Tripon Florin , Bogliş Alina , Făgărăşan Amalia , Bănescu Claudia 
Published: October 2018

Abstract
Small supernumerary marker chromosome (sSMC) is a rare chromosomal abnormality and is detected in about 0.3% in cases with multiple congenital anomalies (MCA) and/or developmental delay. Different techniques for investigation of cases with MCA and/or developmental delay are available ranging from karyotyping to molecular cytogenetic technique and ultimately multiplex ligation dependent probe amplification (MLPA). Here we present a patient with multiple congenital anomalies for which classical cytogenetic technique was used as a first step in diagnosis and the results being confirmed by MLPA. The karyotype disclosed a sSMC considered to be a fragment of chromosome 22. The MLPA analysis using SALSA MLPA probemix P064-C2 Microdeletion Syndromes-1B confirmed the karyotype results, and according to the manufacturer’s recommendation we performed another confirmation analysis with MLPA probemix P311-B1 Congenital Heart Disease and MLPA probemix P250-B2 DiGeorge. We also suspected an Emanuel syndrome and performed another MLPA analysis with SALSA MLPA probemix P036-E3 Subtelomeres Mix 1 and probemix P070-B3 Subtelomeres Mix 2B for investigation of subtelomeric region that revealed a duplication of 11q25 region and the confirmation was performed using SALSA MLPA probemix P286-B2 Human Telomere-11.
In conclusion, we consider that MLPA is a valuable method for identification of sSMC in children with developmental delay and congenital anomalies. Genetic diagnosis using different molecular techniques, such as MLPA, for increasing accuracy in identification of chromosomal structural aberrations has an important role in clinical diagnosis and in genetic counselling and our case explain the importance of using a specific laboratory technique for each stage of diagnosis.
The utility of molecular genetic techniques in craniosynostosis cases associated with intellectual disability
Authors: Bogliş Alina , Tripon Florin , Bănescu Claudia 
Published: October 2018

Abstract
Molecular genetic testing in craniosynostosis leads to the detection of the mutations in the genes encoding fibroblast growth factor receptors (FGFR), providing information about the etiology of the genetic disorder. Muenke syndrome is produced by p.Pro250Arg mutation in FGFR3 gene with evidence of variable expressivity, representing 8% of the syndromic craniosynostoses.
Here, we present the identification of a p.Pro250Arg pathogenic mutation (c.749C>G) in the FGFR3 gene using Multiplex Ligation-dependent Probes Amplification (MLPA) analysis in conjunction with Sanger sequencing in a patient with craniosynostosis and mild intellectual disability. The MLPA analysis detected a reduced signal of the probe, at the site of the c.749C>G mutation, defined by the presence of one allele of C749>G mutation in the FGFR3 gene, exon 7. Sanger sequencing was performed for confirmation and identified heterozygous p.Pro250Arg pathogenic variant (c.749C>G) in exon 7 of the FGFR3.
In conclusion, we assessed the validity and clinical utility of the combined molecular genetic techniques, MLPA analysis, and Sanger sequencing, for craniosynostosis and intellectual disability, improving not only the diagnostic testing but also the genetic counseling and management of the disorder.
Expanding PVL positive MRSA healthcare-associated infections outbreak in a newborns unit, caused by hospital hygiene shortcomings
Authors: Drăgulescu Elena-Carmina , Oprea Mihaela , Zorescu Cătălina , Şerban Roxana , Codiţă Irina 
Published: October 2018

Abstract
A prolonged outbreak of Healthcare-Associated Infections (HCAIs) evolved since December 2013, in a Newborns Unit from Hospital A, sited in the North-Eastern development region, Romania. A first cluster consisted of 19 cases, of which 18 infections in newborns and 1 labour infectious complication in a mother. Except for five cases declared and treated in the Neonatology Unit as hospital-acquired infections, the other cases were discharged and further required rehospitalisation and treatment.
Eight of these innitialy discharged cases were readmitted to the Pediatric Surgery Unit and two others to the Pediatrics Unit of Hospital B, while three others were readmitted to three hospitals: one to the Pediatrics Unit of Hospital C, and other two to Hospital A and Hospital D, respectively. The mother with the labour infectious complication was readmitted to the Gynecology Unit of the Hospital A.
A number of fifteen Staphylococcus aureus (S. aureus) strains isolated from the HCAI first episode and 8 strains from 7 HCWs were received by „Cantacuzino” Institute, Nosocomial Infections and Antibiotic Resistance Laboratory from the County Public Health Directorate, for confirmation and molecular typing.
After a first round of interventions for infection control, a second episode bursted in Hospital A and our laboratory received six other S. aureus isolates from newborns, hospital environment, and HCWs.
Public Health interventions based on epidemiologic data and molecular microbiology results were finally successful. The evolution of all cases was favorable.
An important factor favoring the outbreak was the moving of the Birth Unit of Hospital A to an innapropriate location for an 18-month interval, more than innitially estimated, in relation to rehabilitation of the ward.
We considered to report this episode taking into account the unusual evolution, the risk of multiresistant bacterial strains spreading, and multiple unwanted consequences caused by shortcomings in providing appropriate hygiene conditions.
Instrumental Assessment of the Face Skin Aging in Women
Authors: Manturova Natalia Evgenievna , Stupin Victor Aleksandrovich , Smirnova Galina Olegovna , Silina Ekaterina Vladimirovna 
Published: October 2018

Abstract
The aim of this study is to conduct several non-invasive methods for assessing the level of circulatory disturbance, elasticity and aging of skin in patients of different age groups in order to expand the diagnostic capabilities and evaluate the effectiveness of current research in aesthetic medicine. Clinical and instrumental exploration of 160 women aged 17 to 75 years with varying degrees of involutional skin changes was carried out. To objectify the assessment of skin condition, in all group of patients modern instrumental methods were used, such as: elastometry, ultrasound examination of the skin, laser Doppler flowmetry, transcutaneous oxygen tension. Concurrent implementation of several non-invasive methods for assessing the level of circulatory disturbance, elasticity and aging of the skin, allowed us to find new possibilities for studying the functional state of the skin. These methods extend the possibilities of ultrasonic research methods used today in aesthetic cosmetology. The obtained comparative data of elastometry, ultrasonography, laser Doppler flowmetry and transcutaneous oximetry in patients of different age groups showed the presence of elasticity and structure defect, skin thickness and subcutaneous fat, as well as microcirculation changes since 25 years and marked changes after 40 years.
Effect of Various Agents on the Direction of THP-1 Cell Differentiation
Authors: Zubova Svetlana V. , Radzyukevich Yaroslav V. , Grachev Sergey V. , Prokhorenko Isabela R. 
Published: October 2018

Abstract
The ability of physiological (1α,25-dihydroxyvitamin D3, retinoic acid) and non-physiological (various LPS) agents and their combinations to influence the direction of promonocytic THP-1 cell differentiation was studied.
The differentiating activity of the agents was evaluated by the expression and the ratio of surface receptors (TLR4, CD11b, and CD14) as well as by the change in THP-1 cell phagocytic activity of different degree of differentiation by Flow cytometry.
The THP-1 cell differentiation by VD3 was shown to lead probably to the formation of classical monocytes.
Summarizing we can conclude that VD3 induces the THP-1 cells differentiation with the formation of classical monocytes and the sequence of 1α, 25-dihydroxyvitamin D3 and non-toxic LPS R. capsulatus PG causes the THP-1 cells differentiation with the formation of inflammatory or intermediate monocytes.
Values and Valuations in Judicial Discourse. A Corpus-Assisted Study of (Dis)Respect in US Supreme Court Decisions on Same-Sex Marriage
Authors: Goźdź-Roszkowski Stanisław 
Published: October 2018

Abstract
This paper investigates the role of (DIS)RESPECT a value premise in two landmark civil rights cases given by the United States Supreme Court. It adopts a corpus-assisted approach whereby a keyword analysis and the analysis of key semantic domains are used to identify potential values relied upon by judges in their justifications. The two categories of NO RESPECT and RESPECTED have been selected and examined as one domain of (DIS)RESPECT. (DIS)RESPECT turns out to be the only value marked by strong evaluative polarity and it is found in the majority, as well as in dissenting opinions. The analysis shows how the notion of (DIS)RESPECT has been integrated into the arguments of judges and it highlights the central importance of values and the related evaluative language for legal argumentation.
Frame Modeling Method in Teaching and Learning Legal Terminology
Authors: Ignatkina Anastasia 
Published: October 2018

Abstract
Law is known to exist only being articulated in a language and discourse, and the students’ ability to comprehend and use its meta-language is one of the main goals for English for Legal Purposes (ELP) teaching. The knowledge of terminology enables students to fit new information (linguistic, disciplinary, factual, cultural, etc.) into the framework of the legal system they are studying. The acquisition of terminology in a foreign language implies knowledge of both conceptual content and the means of its verbalization. This article argues for a cognitive approach to teaching Legal English, and frame modelling as an effective method of teaching and learning legal terminology. The heterogeneous structure of legal concepts (a permanent core and dynamic periphery) suggests the possibility of framing their verbal representations. From this perspective, legal terminology is viewed as a frame structure. Depending on the instructional objective, frame modelling may be circumscribed around a specific concept or frame level.
Affixed Terms in Cognitive Categorization of the Legal Picture of the World and in LSP Teaching
Authors: Khizhnyak Sergey 
Published: October 2018

Abstract
The interdisciplinary notion picture of the world makes research works devoted to this area of studies challenging from the point of view of finding interconnections between linguistic and extra-linguistic factors in the process of structuring categories of words, including those functioning in terminological systems and subsystems. Legal pictures of the world are specific cultural phenomena that may differ in various countries due to the nationally specific features of law and legal culture development. One of the most complicated problems of representing specific knowledge through linguistic, terminological, signifiers is the problem of linguistic categorization of concepts. The article considers the problem of forming legal categories based on word-building suffixes from the point of view of the development of the legal language ‘picture of the world’ [reflection of the world] closely associated with its conceptual picture. The conclusion is drawn that commonly used derivational suffixes transform their meanings in accordance with the legal taxonomies, forming the conceptual legal picture of the world. The results of theoretical studies in terminological conceptualization may be used in teaching legal English vocabulary in the form of systematic presentation of term-building patterns expressing certain conceptual categories.
The Journey from Untouchable to Dalit: Pioneering Literary Landmarks and Dissident Dalit Voices of Contemporary India
Authors: García-Arroyo Ana 
Published: October 2018

AbstractThis paper analyses the situation of Untouchable / Dalit people in India through intersecting literature and social realities. It focuses on the most relevant and pioneering literary works of colonial and postcolonial times and how these landmarks of fiction function as a mimetic expression of everyday life. Then, the main objective is 1) to give an overview of the representation of untouchability and its evolution into the Dalit consciousness within the interrelated contexts of literature and real life; and 2) to demonstrate that in much less than a century India has witnessed astonishing changes as far as the social stratification of caste-gender is concerned. Keywords: untouchability, Dalit identity, pioneering Dalit literature, gender-caste discrimination ResumenEste artículo analiza la situación de los/as intocables o Dalits en India, interrelacionando realidades literarias y sociales. Se examinan las obras literarias pioneras de la etapa colonial y postcolonial y cómo éstos textos de ficción mimetizan la realidad diaria. Así, el objetivo principal es 1) exponer cómo se ha representado la intocabilidad y cuál ha sido su evolución hasta la formación de la consciencia Dalit, en el contexto literario y social; y 2) demostrar que en menos de un siglo se han producido asombrosos cambios en lo que se refiere a la categoría social de casta-género. Palabras clave: intocabilidad, identidad Dalit, literatura pionera Dalit, discriminación de género-casta.
La corrección política y el control ideológico-cognitivo de la realidad
Authors: Chamizo Domínguez Pedro Jesus , Reutner Ursula 
Published: October 2018

Resumen Aunque el término corrección política es relativamente reciente, el asunto de la propia corrección política parece estar extendido en todas las culturas y en todas las épocas. No obstante, la consideración de qué sea lo políticamente correcto varía en función de las diferentes época, a otra, culturas (o lenguas) y de cómo nos enfrentamos cognitivamente a la realidad. Y esto ocurre por cuanto que cada grupo social intenta imponer al resto de los grupos sociales sus propios criterios sobre qué términos son políticamente correctos –y por tanto deben ser usados para expresar las propias creencias ideológicas– y qué términos son políticamente incorrectos –y por tanto deben ser censurados en la medida en que parecen atentar contra las propias creencias ideológicas. Y ello a pesar del famoso adagio latino “Verba non mutant substantiam rei”. Palabras clave: corrección política, control ideológico, cambios semánticos. Abstract In spite of the fact that the term political correctness is relatively recent, the matter of political correctness itself seems to be widespread all over the cultures and all over the epochs. However, the consideration of what is politically correct differs according to the different epochs, cultures (or languages) and according to how we cognitively face up to reality. And this happens inasmuch as every social group tries to impose the rest of social groups its own criteria about what terms are politically correct – and consequently have to be used in order to show their own ideological beliefs – and what terms are politically incorrect – and consequently have to be censored to the extent that they seem to infringe upon their own ideological beliefs. And this despite the well-known Latin adage: “Verba non mutant substantiam rei”. Key words: political correctness, ideological control, semantic changes.
PENGEMBANGAN PEMAHAMAN DAN KEMAMPUAN RESOLUSI KONFLIK DENGAN METODE STUDI KASUS PADA MATA KULIAH PENDIDIKAN KEWARGANEGARAAN DI STKIP PGRI SIDOARJO
Authors: Kurniawan Agung 
Published: October 2018

<p class="E-JOURNALAbstractBody">Tujuan dari penelitian ini adalah untuk Mengetahui pemahaman dan kemampuan resolusi konflik mahasiswa. Penelitian ini dilatarbelakangi dengan meningkatnya tingkat konflik di semua jenjang pendidikan. Selain itu, rendahnya pemahaman terhadap cara-cara dalam menyelesaikan konflik. Penelitian ini menggunakan penelitian tindakan kelas. data dianalisa dengan cara deskriptif kualitatif dengan metode triangulasi data<span lang="IN">.</span> Pada siklus I pemahaman dan kemampuan resolusi konflik mahasiswa sekitar 50%. Berdasarkan hasil evaluasi dari siklus I tersebut, perlu diadakan penambahan dan perubahan pada materi dan metode. Pada siklus II tingkat pemahaman dan kemampuan resolusi konflik mahasiswa meningkat menjadi 79.5%. Hal tersebut dikarenakan adanya penambahan materi dan perubahan metode yaitu dengan memakai studi kasus. </p>
Turfan Uygurs’ Contributions to Mahāyāna Buddhist Material Culture and Art Renaissance
Authors: ZEREN Münevver Ebru 
Published: October 2018

Turks contributed highly in development of Buddhist religion, philosophy and culture in Turkestan and China starting from the dynasties they established in North China. Many of the first Buddhist sūtras were translated from Indian languages to Chinese and the first Buddhist cave temples were built under the patronage of these Turks’ sovereign. Buddhist Uygurs made great contributions, especially to Mahāyāna Buddhism in Turfan and Dunhuang. The renaissance of Mahāyāna Buddhist Art which flourished in Northern India by Kushans happened in Turfan 10th century onwards. This artistic revival in the ancient Buddhist center stressed especially Buddhahood and paradise themes. Pranidhi scenes gained their gigantic dimensions with Uygurs. The emphasized artistic themes and their new representations have strong links with old Turkic culture and religion. In this paper, we tried to analyze the emergence of some important Mahāyāna Buddhist material culture themes and symbols used by Turfan Uygurs and their contribution to Mahāyāna Buddhist Art Renaissance. As we tried to interpret this Buddhist culture links to the past, we expect to shed light on cultural history of Turfan Uygurs, which continued some of their old beliefs and religious ideas after adopted Buddhism and merged them into Buddhist material culture.
Beyaz ve kahverengi ticari yumurtacı tavuklarda, tavuk yaşı ve kafes katının yumurta dış kalite parametreleri üzerine etkileri
Authors: AKKUŞ Bayram , YILDIRIM İskender 
Published: October 2018

Bu
çalışmanın amacı, değişik yaş gruplarında ve farklı kafes katlarında
yetiştirilen beyaz ve kahverengi yumurta tavuklarından elde edilen yumurtaların
dış kalite özelliklerinin belirlenmesi/karşılaştırılmasıdır.&nbsp; Çalışmalarda ticari yumurtacı Nick-Chick ve
Nick- Brown hibritlerinde farklı kafes katlarında (1, 3, 5. katlarda) 28, 52 ve
70 haftalık yaşlarda elde edilen yumurtaların dış kalite özellikleri arasındaki
farklılıklar incelenmiştir. Dış kalite kriterleri olarak,&nbsp; yumurta ağırlığı, özgül ağırlık, şekil
indeksi, kabuk kalınlığı, kabuk kırılma mukavemeti ve yumurta kabuk yüzey alanı
değerleri belirlenmiştir. Sonuçta, tavuklarda genotip, yaş ve kafes katının
yumurta ağırlığı, özgül ağırlık ve kabuk özelliklerine önemli etkileri
bulunmuştur (P&lt;0,01). Kafes katlarının yumurta kalitesine etkileri, birinci
katın yumurta ağırlığı, şekil indeksi, kabuk kalınlığı, kabuk yüzey alanı,
parametreleri bakımından diğer katlara (3-5) göre daha yüksek olarak tespit
edilmiştir (P&lt;0.01). Yumurta tavuklarında yaş ilerledikçe yumurta
ağırlığının ve özgül ağırlığın arttığı tespit edilmiştir.
To Determine the Correlation between Anthropometric Variables of Obesity and Hypertension at NGMC: A Tertiary Care Hospital
Authors: Neupane Pant Bhawana , Pant Manoj , Goit Rajesh Kumar , Neupane Ashish , Neupane Ganesh Prasad , Bhargava Pushpa 
Published: October 2018

Aim: Obesity is associated with the metabolic risk factors such as high blood pressure, body fat abnormality, and glucose intolerance which may influence the morbidity and mortality due to cardiovascular diseases. The present study is to determine the correlation of common anthropometric indices of obesity with blood pressure among population in mid-western region of Nepal. The result will provide us insight on relevant anthropometric indices for predicting hypertension based on linear correlation calculated from our data. Methods: We screened 500 healthy subjects to study the correlation between anthropometric indices and blood pressure.Result: Our result showed strong correlation between Sum of Skin fold, waist circumference, and waist hip ratio with blood pressure (systolic blood pressure, diastolic blood pressure and mean arterial blood pressure). However some of the anthropometric indices like hip circumference and body mass index did not have significant correlation with blood pressure. Conclusion: Provided obesity related morbidity and mortality is burgeoning in our society it is imperative to identify right indicators of cardiovascular disease for specific population. Relevant anthropometric indices can serve as excellent indicators if used based of scientific validation. JNGMC, Vol. 14 No. 2 December 2016, Page:70-74
The corrosion behavior of porous Ni–Cr–Fe alloy in 2 mol L−1 alkaline solution
Authors: Wu Liang , Li Xiaona , Yang Ge , Tang Zhi , Guo Xiaohua , Xu Yang , Xiao Yifeng , Qian Jinwen , Xu Yanfei , He Yuehui 
Published: October 2018

Abstract
Ni–Cr–Fe alloy samples with different porosities were prepared via the Kirkendall effect, with a reactive synthesis of Ni, Cr, and Fe elemental powders. The microstructure, surface morphology and the compositions were analyzed with X-ray diffraction and scanning electron microscopy. The corrosion behavior was explored in a 2 mol L−1 KOH solution using electrochemical methods and an immersion test. The polarization resistance indicated that the free corrosion potential and the lower corrosion current of the porous Ni–Cr–Fe alloy were −0.18528 V and 3.5998 × 10−7 A cm−2. The immersion test showed that after 25 days of immersion, the weight change of porous Ni–Cr–Fe alloy was 0.132 %, which was less than the porous Ni (0.346 %). X-ray photoelectron spectroscopy results revealed the corrosion products of the porous Ni–Cr–Fe alloy and the porous Ni. The corrosion mechanism of the porous Ni–Cr–Fe alloy was discussed.
The grain growth in alumina compacts sintered under high pressure
Authors: Ozturk Zeynep Taslicukur , Kuskonmaz Nilgun 
Published: October 2018

Abstract
In this study, alumina ceramics were produced with high density and nanograin size without additives from nano-size γ-Al2O3. The effects of sintering pressure (3 GPa and 7 GPa) and temperature (600 °C and 1 200 °C) on the grain size of sintered samples were studied. It was shown that high pressure increases the nucleation rate while reducing the growth rate of the transformed α phase. Pressure-assisted densification decreases grain size, so that nano-scale grains can be achieved in the sintered structure. The effect of various sintering conditions on grain growth in alumina was investigated during high-pressure sintering for better understanding of pressure and temperature effects on microstructural properties.