bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interactive effects of dietary fishmeal level and plant essential oils supplementation on European sea bass, Dicentrarchus labrax: Growth performance, nutrient utilization, and immunological response
Authors: Gonçalves Rui A. , Serradeiro Renata , Machado Marina , Costas Benjamin , Hunger Christine , Dias Jorge 
Published: May 2019

AbstractThe inclusion of phytogenics in fish feed is a promising strategy to compensate for the negative performance effects of replacing fishmeal (FM) with vegetable sources. The present work assessed the interactive effects of different dietary FM levels (22.5 and 10% of formulation) and the supplementation of a commercial blend of anise, citrus, and oregano essential oils (Digestarom PEP M.G.E 150) on European sea bass, Dicentrarchus labrax growth performance, nutrient utilization, gut morphology, antioxidant status, and immunological response over a 60‐day growth trial. Results showed decreased growth and protein efficiency ratio and increased feed conversion ratio with a low dietary FM level. In contrast, supplementation of the phytogenic product demonstrated improved performance and nutrient utilization together with increased protein and energy retention. Supplementation with the plant essential oils fully compensated for the negative intestinal changes observed in sea bass fed a low‐FM diet but showed little improvement in fish immunological response, except for the 30% increase in lysozyme activity observed in fish fed the low FM‐supplemented diet compared to those fed the standard high‐FM diet. Overall, this study supports the use of this phytogenic product in low‐FM diets as a possible tool to decrease feed costs associated with FM without compromising fish performance, nutrient utilization, and health.
Lineage specific histories of Mycobacterium tuberculosis dispersal in Africa and Eurasia
Authors: O'Neill Mary B. , Shockey Abigail , Zarley Alex , Aylward William , Eldholm Vegard , Kitchen Andrew , Pepperell Caitlin S. 
Published: May 2019

AbstractMycobacterium tuberculosis (M.tb) is a globally distributed, obligate pathogen of humans that can be divided into seven clearly defined lineages. An emerging consensus places the origin and global dispersal of M.tb within the past 6,000 years: identifying how the ancestral clone of M.tb spread and differentiated within this timeframe is important for identifying the ecological drivers of the current pandemic. We used Bayesian phylogeographic inference to reconstruct the migratory history of M.tb in Africa and Eurasia and to investigate lineage specific patterns of spread from a geographically diverse sample of 552 M.tb genomes. Applying evolutionary rates inferred with ancient M.tb genome calibration, we estimated the timing of major events in the migratory history of the pathogen. Inferred timings contextualize M.tb dispersal within historical phenomena that altered patterns of connectivity throughout Africa and Eurasia: trans‐Indian Ocean trade in spices and other goods, the Silk Road and its predecessors, the expansion of the Roman Empire, and the European Age of Exploration. We found that Eastern Africa and Southeast Asia have been critical in the dispersal of M.tb. Our results further reveal that M.tb populations have grown through range expansion, as well as in situ, and delineate the independent evolutionary trajectories of bacterial subpopulations underlying the current pandemic.
Unravelling the lipoyl‐relay of exogenous lipoate utilization in Bacillus subtilis
Authors: Rasetto Natalí B. , Lavatelli Antonela , Martin Natalia , Mansilla María Cecilia 
Published: May 2019

SummaryLipoate is an essential cofactor for key enzymes of oxidative and one‐carbon metabolism. It is covalently attached to E2 subunits of dehydrogenase complexes and GcvH, the H subunit of the glycine cleavage system. Bacillus subtilis possess two protein lipoylation pathways: biosynthesis and scavenging. The former requires octanoylation of GcvH, insertion of sulfur atoms and amidotransfer of the lipoate to E2s, catalyzed by LipL. Lipoate scavenging is mediated by a lipoyl protein ligase (LplJ) that catalyzes a classical two‐step ATP‐dependent reaction. Although these pathways were thought to be redundant, a ∆lipL mutant, in which the endogenous lipoylation pathway of E2 subunits is blocked, showed growth defects in minimal media even when supplemented with lipoate and despite the presence of a functional LplJ. In this study, we demonstrate that LipL is essential to modify E2 subunits of branched chain ketoacid and pyruvate dehydrogenases during lipoate scavenging. The crucial role of LipL during lipoate utilization relies on the strict substrate specificity of LplJ, determined by charge complementarity between the ligase and the lipoylable subunits. This new lipoyl‐relay required for lipoate scavenging highlights the relevance of the amidotransferase as a valid target for the design of new antimicrobial agents among Gram‐positive pathogens.
Resource‐ratio theory predicts mycorrhizal control of litter decomposition
Authors: Smith Gabriel R. , Wan Joe 
Published: May 2019

Summary

Ecosystems with ectomycorrhizal plants have high soil carbon : nitrogen ratios, but it is not clear why. The Gadgil effect, where competition between ectomycorrhizal and saprotrophic fungi for nitrogen slows litter decomposition, may increase soil carbon. However, experimental evidence for the Gadgil effect is equivocal.

Here, we apply resource‐ratio theory to assess whether interguild fungal competition for different forms of organic nitrogen can affect litter decomposition. We focus on variation in resource input ratios and fungal resource use traits, and evaluate our model's predictions by synthesizing prior experimental literature examining ectomycorrhizal effects on litter decomposition.

In our model, resource input ratios determined whether ectomycorrhizal fungi suppressed saprotrophic fungi. Recalcitrant litter inputs favored the former over the latter, allowing the Gadgil effect only when such inputs predominated. Although ectomycorrhizal fungi did not always hamper litter decomposition, ectomycorrhizal nitrogen uptake always increased carbon : nitrogen ratios in litter.

Our meta‐analysis of empirical studies supports our theoretical results: ectomycorrhizal fungi appear to slow decomposition of leaf litter only in forests where litter inputs are highly recalcitrant. We thus find that the specific contribution of the Gadgil effect to high soil carbon : nitrogen ratios in ectomycorrhizal ecosystems may be smaller than predicted previously.
Asymmetric nasomaxillary expansion induced by tooth‐bone‐borne expander producing differential craniofacial changes
Authors: Kim Kyung‐A , Oh Song‐Hee , Kim Byoung‐Ho , Kim Su‐Jung 
Published: May 2019

AbstractObjectivesTo evaluate three‐dimensional (3D) craniofacial changes induced by a non‐surgical tooth‐bone‐borne rapid palatal expander (TBB‐RPE) according to the symmetrical pattern of expansion, to investigate the 3D changes between the sides in patients with asymmetric expansion, and to identify the related factors of asymmetric expansion.Setting and sample populationSixty‐six patients (mean age: 19.3 ± 5.7 years) treated with TBB‐RPE were divided into a symmetric expansion group (Group S, n = 46) or asymmetric expansion group (Group A, n = 20). Group S was subdivided into Group Ss (n = 27), with bilateral frontomaxillary suture (FMS) split, and Group Sn (n = 19), with no FMS split.Materials and methodsPre‐ and post‐expansion cone‐beam computed tomography images were superimposed, and the common coordinated system was set. All landmarks were designated as coordinate pairs, and treatment changes were automatically calculated. Analysis of variance was conducted for intergroup comparison of craniofacial changes, and logistic regression analysis was performed to identify the related factors of asymmetric expansion.ResultsThe frequency of asymmetric expansion was 30.3%. Group A with unilateral FMS split showed less craniofacial changes than Group Ss and more changes than Group Sn. Group A exhibited different nasomaxillary displacement between the two halves, showing greater changes in the FMS‐split side. Among the tested six variables (age, gender, Angle's classification, unilateral crossbite, maxillary cant and chin deviation), chin deviation was uniquely associated with asymmetric expansion.ConclusionsTooth‐bone‐borne rapid palatal expander had a risk of asymmetric expansion, especially in facial asymmetric patients with chin deviation, producing different craniofacial changes from symmetric expansion.
Intraoperative blood loss and transfusion during primary pediatric liver transplantation: A single‐center experience
Authors: Villarreal Joshua A. , Yoeli Dor , Ackah Ruth L. , Sigireddi Rohini R. , Yoeli Jordan K. , Kueht Michael L. , Galvan N Thao N. , Cotton Ronald T. , Rana Abbas , O’Mahony Christine A. , Goss John A. 
Published: May 2019

AbstractChildren undergoing liver transplantation are at a significant risk for intraoperative hemorrhage and thrombotic complications, we aim to identify novel risk factors for massive intraoperative blood loss and transfusion in PLT recipients and describe its impact on graft survival and hospital LOS. We reviewed all primary PLTs performed at our institution between September 2007 and September 2016. Data are presented as n (%) or median (interquartile range). EBL was standardized by weight. Massive EBL and MT were defined as greater than the 85th percentile of the cohort. 250 transplantations were performed during the study period. 38 (15%) recipients had massive EBL, and LOS was 31.5 (15‐58) days compared to 11 (7‐21) days among those without massive EBL (P < 0.001). MT median LOS was 34 (14‐59) days compared to 11 (7‐21) days among those without MT (P = 0.001). Upon backward stepwise regression, technical variant graft, operative time, and transfusion of FFP, platelet, and/or cryoprecipitate were significant independent risk factors for massive EBL and MT, while admission from home was a protective factor. Recipient weight was a significant independent risk factor for MT alone. Massive EBL and MT were not statistically significant for overall graft survival. MT was, however, a significant risk factor for 30‐day graft loss. PLT recipients with massive EBL or MT had significantly longer LOS and increased 30‐day graft loss in patients who required MT. We identified longer operative time and technical variant graft were significant independent risk factors for massive EBL and MT, while being admitted from home was a protective factor.
Engineered Functional Recovery of Microbial Rhodopsin Without Retinal‐Binding Lysine
Authors: Yamauchi Yumeka , Konno Masae , Yamada Daichi , Yura Kei , Inoue Keiichi , Béjà Oded , Kandori Hideki 
Published: May 2019

AbstractDefinition of rhodopsin is the retinal‐binding membrane protein with the Schiff base linkage at a lysine on the 7th transmembrane helix. However, ~ 600 microbial rhodopsins lack retinal‐binding lysine at the corresponding position (Rh‐noK) among ~ 5500 known microbial rhodopsins, suggesting that Rh‐noK has each functional role without chromophore. Here, we report successful functional recovery of Rh‐noK. Two Rh‐noKs from bacteria were heterologously expressed in Escherichia coli, which exhibited no color. When retinal‐binding lysine was introduced, one of them gained visible color. Additional mutation of the Schiff base counterion further gained proton‐pumping activity. Successful engineered functional recovery such as visible color and proton‐pump activity suggests that the Rh‐noK protein forms a characteristic structure of microbial rhodopsins.
Persistence and determinants of income inequality: The Brazilian case
Authors: Signor Diogo , Kim Jongsung , Tebaldi Edinaldo 
Published: May 2019

AbstractLatin America, which is a region known for its high and persistent income inequality levels, experienced a significant decline in income inequality since the second half of the 1990s. Brazil is a particularly interesting case in Latin America. While the country presented notable economic growth and improvements in income distribution in the early 2000s, Brazil continues to experience high levels of income inequality in comparison with other Latin American or advanced economies. This research contributes to the literature by examining the key drivers of income distribution and the degree of persistence of income inequality among Brazilian states. This research also improves upon previous works by using more recent and comprehensive data and addressing concerns regarding heterogeneity and endogeneity by using the system GMM estimation method. Our findings show that income inequality is highly persistent across Brazilian states and that government policies including income transfer programs made important contributions to reduce income inequality in Brazil. This study also shows that the decline in labor income ratios between different ethnic groups and the increase of the share of formal jobs in the labor market contributed to reduce income inequality.
Remittances and healthcare expenditure: Human capital investment or responses to shocks? Evidence from Peru
Authors: Berloffa Gabriella , Giunti Sara 
Published: May 2019

AbstractThis paper estimates the effect of international remittances on healthcare expenditures, taking into account both the interdependence with other consumption goods and the effects of health shocks. More precisely, we assess whether the budget allocation decisions of remittance‐receiving households reveal different preferences to invest in health capital, even when the simultaneous effect that health shocks may have on the demand of remittances and on other types of nondurable expenditures is accounted for. Using data from the “Peruvian National Survey of Households,” we find that remittances have a positive impact on healthcare budget shares, net of the remittance‐related income effect and independently of the occurrance of a health shock. They also have a positive impact on housing and a negative one on other expenditure items, that is, clothing, transport, and education. Hence, our results indicate a “pure” tendency of remittance‐receiving households to devote larger shares of their budget to health capital investment, rather than to other types of consumption goods.
Outcomes of processing of bone marrow harvests for hematopoietic stem cell transplantation in pediatric patients utilizing a novel red blood cell sedimentation kit
Authors: Akel Salem , Murray Christianna , Ferguson William , Babic Aleksandar 
Published: May 2019

BACKGROUNDBone marrow (BM) grafts are often subject to manipulation to minimize infusion volume, to deplete red blood cells (RBCs), to deplete plasma, and/or to achieve optimal concentration of nucleated cells (NCs) for freezing. Here, we report results of processing and infusion of four major ABO‐incompatible BM grafts for allogeneic hematopoietic transplantation in pediatric patients.CASES AND METHODSPatients ranging in age from 5 months to 18 years received hematopoietic stem cell transplantation for benign or malignant indications. Allogeneic BM grafts were processed using PrepaCyte‐CB kits, approved by the Food and Drug Administration for enrichment of mononuclear cells in cord blood units for hematopoietic transplantation. Resulting grafts were dosed and infused using standard institutional protocol. After transplantation patients were evaluated for hematologic recovery.RESULTSThe PrepaCyte‐CB processing method achieved on average 97.1% depletion of RBCs and 68.5% volume reduction, with a fairly efficient NC recovery (total NCs, 85.6%; white blood cells, 89.7%; CD34+ cells, 103.3%; and colony‐forming unit cells, 87.5%). No microbial contamination was detectable in any of the processed grafts. Infusions were well tolerated, and all four patients achieved durable hematopoietic engraftment within expected time interval.CONCLUSIONOur results suggest that PrepaCyte‐CB is safe and effective for minimal processing of BM grafts. Engraftment outcomes were comparable with other published BM processing methods. In addition, NC recovery achieved using this method appears to exceed that of previously described sedimentation reagents such as hetastarch and Ficoll.
Mercado del suelo urbano y producción del espacio residencial en la ciudad de Tandil, Argentina
Authors: Migueltorena Alejandro , Linares Santiago 
Published: May 2019

El siguiente trabajo se propone interpretar la situación actual del mercado del suelo y la vivienda en la ciudad de Tandil. Para este fin, se utilizará la teoría de la renta del suelo urbano, la cual permite conocer los procesos subyacentes en la conformación del mercado de la vivienda en las ciudades, la presencia de las distintas clases de rentas en la conformación de los precios de los inmuebles y la incidencia que éstas poseen para explicar la disposición espacial de las áreas residenciales.
Se propone, además, una tipología habitacional que permite caracterizar y dar cuenta de las diversas fuerzas que convergen en las áreas residenciales de la ciudad de Tandil, una aglomeración intermedia de la provincia de Buenos Aires, Argentina. Con este objetivo, se utilizan fuentes de información primaria y datos del Censo Nacional de Población, Hogares y Viviendas de 2010 (Instituto Nacional de Estadísticas y Censos [Indec], 2010). La cartografía es confeccionada mediante Sistemas de Información Geográfica.
Syn-collisional exhumation of hot middle crust in the Adirondack Mountains (New York, USA): Implications for extensional orogenesis in the southern Grenville province
Authors: Regan S.P. , Walsh G.J. , Williams M.L. , Chiarenzelli J.R. , Toft M. , McAleer R. 
Published: May 2019

Abstract
Extensional deformation in the lower to middle continental crust is increasingly recognized and shown to have significant impact on crustal architecture, magma emplacement, fluid flow, and ore deposits. Application of the concept of extensional strain to ancient orogenic systems, like the Grenville province of eastern North America, has helped decipher the structural evolution of these regions. The Marcy massif is a ∼3000 km2 Mesoproterozoic anorthosite batholith in the Adirondack Mountains (New York, USA) of the southern Grenville province. Bedrock geology mapping at 1:24,000 scale paired with characterization of bedrock exposed by recent landslides provides a glimpse into the structural architecture of the massif and its margin. New data demonstrate granulite- to amphibolite-facies deformational fabrics parallel the margin of the batholith, and that the Marcy massif is draped by a southeast-directed detachment zone. Within the massif, strain is localized into mutually offsetting conjugate shear zones with antithetic kinematic indicators. These relationships indicate that strain was coaxial within the Marcy massif, and that subsimple shear components of strain were partitioned along its margin. In situ U–Th–total Pb monazite analysis shows that deformation around and over the Marcy massif occurred from 1070 to 1060 Ma during granulite-facies metamorphism, and monazite from all samples record evidence for fluid-mediated dissolution reprecipitation from 1050 to 980 Ma. We interpret that rocks cooled isobarically after accretionary orogenesis and emplacement of the anorthosite-mangerite-charnockite-granite plutonic suite at ca. 1160–1140 Ma. Gravitational collapse during the Ottawan phase of the Grenville orogeny initiated along a southeast-directed detachment zone (Marcy massif detachment zone), which accommodated intrusion of the Lyon Mountain Granite Gneiss, and facilitated substantial fluid flow that catalyzed the formation of major ore deposits in the Adirondack Highlands.
Early Paleozoic post-breakup magmatism along the Cordilleran margin of western North America: New zircon U-Pb age and whole-rock Nd- and Hf-isotope and lithogeochemical results from the Kechika group, Yukon, Canada
Authors: Campbell Roderick W. , Beranek Luke P. , Piercey Stephen J. , Friedman Richard 
Published: May 2019

AbstractPost-breakup magmatic rocks are recognized features of modern and ancient passive margin successions around the globe, but their timing and significance to non-plume-related rift evolution is generally uncertain. Along the Cordilleran margin of western North America, several competing rift models have been proposed to explain the origins of post-breakup igneous rocks that crop out from Yukon to Nevada. New zircon U-Pb age and whole-rock geochemical studies were conducted on the lower Paleozoic Kechika group, south-central Yukon, to test these rift models and constrain the timing, mantle source, and tectonic setting of post-breakup magmatism in the Canadian Cordillera. The Kechika group contains vent-proximal facies and sediment-sill complexes within the Cassiar platform, a linear paleogeographic high that developed outboard of continental shelf and trough basins. Chemical abrasion (CA-TIMS) U-Pb dates indicate that Kechika group mafic rocks were generated during the late Cambrian (488–483 Ma) and Early Ordovician (473 Ma). Whole-rock trace-element and Nd- and Hf-isotope results are consistent with the low-degree partial melting of an enriched lithospheric mantle source during margin-scale extension. Equivalent continental shelf and trough rocks along western North America are spatially associated with transfer-transform zones and faults that were episodically reactivated during Cordilleran rift evolution. Post-breakup rocks emplaced along the magma-poor North Atlantic margins, including those near the Orphan Knoll and Galicia Bank continental ribbons, are proposed modern analogues for the Kechika group. This scenario calls for the release of in-plane tensile stresses and off-axis, post-breakup magmatism along the nascent plate boundary prior to the onset of seafloor spreading.
Embolisation of branches of the superior mesenteric artery in the treatment of haemosuccus pancreaticus
Authors: Raymundo Selma Regina de Oliveira , da Silva Gabriela Leopoldino , Reis Luiz Fernando , Freire Antonio Fernandes 
Published: May 2019

Haemosuccus pancreaticus (HP) is an uncommon cause of upper gastrointestinal (GI) bleeding, most often intermittent, making it difficult to diagnose, becoming fatal. It usually occurs in patients with chronic pancreatitis and is caused by the rupture of a visceral aneurysm within the main pancreatic duct. The association between pseudoaneurysm formation and pancreatitis is well established. Pseudoaneurysm occurs in 3.5%–10% of pancreatitis cases and its rupture is a rare but life-threatening complication of chronic pancreatitis occurring in 6%–8% of patients with pseudocysts and corresponds to less than 1% of cases of GI bleeding.Its diagnosis is challenging, given the intermittent nature of bleeding. Angiographic therapy is considered the first-choice treatment, especially in patients who are stable haemodynamically. We present a case of embolisation of inferior pancreaticoduodenal branches with polyvinyl alcohol microparticles in the treatment of HP.