bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative functional genomics decodes herpes simplex virus 1
Authors: Whisnant Adam W. , Jürges Christopher S. , Hennig Thomas , Wyler Emanuel , Prusty Bhupesh , Rutkowski Andrzej J , L’hernault Anne , Göbel Margarete , Döring Kristina , Menegatti Jennifer , Antrobus Robin , Matheson Nicholas J. , Künzig Florian W.H. , Mastrobuoni Guido , Bielow Chris , Kempa Stefan , Chunguang Liang , Dandekar Thomas , Zimmer Ralf , Landthaler Markus , Grässer Friedrich , Lehner Paul J. , Friedel Caroline C. , Erhard Florian , Dölken Lars 
Published: April 2019

SummarySince the genome of herpes simplex virus 1 (HSV-1) was first sequenced more than 30 years ago, its predicted 80 genes have been intensively studied. Here, we unravel the complete viral transcriptome and translatome during lytic infection with base-pair resolution by computational integration of multi-omics data. We identified a total of 201 viral transcripts and 284 open reading frames (ORFs) including all known and 46 novel large ORFs. Multiple transcript isoforms expressed from individual gene loci explain translation of the vast majority of novel viral ORFs as well as N-terminal extensions (NTEs) and truncations thereof. We show that key viral regulators and structural proteins possess NTEs, which initiate from non-canonical start codons and govern subcellular protein localization and packaging. We validated a novel non-canonical large spliced ORF in the ICP0 locus and identified a 93 aa ORF overlapping ICP34.5 that is thus also deleted in the FDA-approved oncolytic virus Imlygic. Finally, we extend the current nomenclature to include all novel viral gene products. Taken together, this work provides a valuable resource for future functional studies, vaccine design and oncolytic therapies.
Preparation and characterization of phospholipid stabilized nanoemulsions in small-scale
Authors: Gurung Shila , Holzer Martin , Barnert Sabine , Schubert Rolf 
Published: April 2019

AbstractPhospholipids have been used to prepare liposomes. The use of phospholipids to stabilize nanoemulsions may cause spontaneous formation of liposomes. The main objective of this study is to develop a method to prepare phospholipid stabilized nanoemulsions in small scale (< 1 mL) and to minimize the formation of liposomes.A combination of hand extrusion and detergent removal methods was used in this study. Extrusion through polycarbonate membranes was performed in two steps, firstly using membranes of 400 nm followed by 200 nm membranes as the second step. Sodium cholate was used as a detergent to solubilize the formed liposomes which was later removed via dialysis. Nanoemulsions were characterized by measuring their particle size, polydispersity index and zeta-potential using Photon Correlation Spectroscopy and Cryo-TEM pictures. The stability of nanoemulsion stored under refrigeration was also studied.Fifty-one extrusion cycles through polycarbonate membrane of 400 nm pore size followed by one-hundred fifty-three cycles through polycarbonate membrane of 200 nm produced nanoemulsions having particle size below 200 nm (diameter). The nanoemulsions were found to be homogenous as depicted by polydispersity index (PDI) value below 0.1. Similarly, the zeta-potential was measured to be above −30 mV which is sufficient to keep nanoemulsions stable for as long as 7 months when stored under refrigeration. The Cryo-TEM pictures revealed 30 mM to be an optimum concentration of sodium cholate to prepare homogenous nanoemulsions with negligible proportion of liposomes.It was concluded that this method could be established as a small scale method of preparing nanoemulsions which will not only reduce the cost of preparation but also the disposal cost of toxic chemicals used for functionalizing nanoemulsions for scientific research.
Modulated Zika virus NS1 conjugate offers advantages for accurate detection of Zika virus specific antibody in double antigen binding and Ig capture enzyme immunoassays
Authors: Tedder Richard S , Dicks Steve , Ijaz Samreen , de Souza Nathalia Caroline Santiago , de Paula Anderson Vincente , Levy Flavia , Medialdea-Carrera Raquel , Levi José Eduardo , Pannuti Claudio S , de Sequeira Patrícia Carvalho , Brown David W G , Lumb Ines Ushiro 
Published: April 2019

AbstractThe accurate diagnosis and seroprevalence investigations of Zika virus (ZKV) infections remain complex due to cross reactivity with other flaviviruses. Two assay formats, both using labelled Zika virus NS1 antigen as a revealing agent (a double antigen binding assay, DABA, and an immunoglobulin Ig capture assay, IgG capture) were initially developed and compared with the indirect EuroimmunZ assay for the detection of anti-Zika antibody. Of 147 pre-Zika period serum samples, 39 (27%) were reactive in the EuroimmunZ or the DABA assays, 28 sera concordantly so. Such false reactivity was influenced by the serotype of Dengue virus (DV) to which individuals had been exposed to. Thus, of sera from patients undergoing secondary Dengue virus infection of known serotype, 91%, 45% and 28% of Dengue virus serotype 2, 3 and 4 respectively were reactive in one or more of the three assays. A novel method of quenching false sero-reactivity was therefore developed for the DABA and IgG capture assays. Initial addition of a single homologous Dengue virus serotype 3 NS1Ag quench significantly ablated false reactivities in the pre-Zika period sera. An equipotent quadrivalent quench comprising homologous Dengue virus serotypes 1 to 4 NS1Ag was shown to be optimum yet retained sensitivity for the detection of specific anti-Zika antibody. Comparing DABA and IgG capture assays using quenched and unquenched conjugates in comparison with EuroimmunZ early in the course of PCR-confirmed infection indicated that a significant component of the apparent early anti-ZIKA antibody response is likely to be due to a Zika virus-driven anamnestic anti-Dengue virus response. The increased specificity provided by homologous antigen quenching is likely to provide a significant improvement in sero-diagnostics and to be of clinical value.
The physiological response in the mammary gland to Escherichia coli infection reflects a conflict between glucose need for milk production and immune response
Authors: Blum Shlomo E. , Heller Dan E. , Jacoby Shamay , Krifucks Oleg , Merin Uzi , Silanikove Nissim , Leitner Gabriel 
Published: April 2019

AbstractUpon entry into the mammary gland, mammary pathogenic strains of Escherichia coli rapidly grow using milk as a nutrient source, in a manner highly dependent on the expression of the ferric-dicitrate system by these bacteria. Intra-mammary challenge with distinct mammary pathogenic E. coli (MPEC) strains result in development of clinical acute mastitis, with peak bacterial counts in milk at 16-24 h post-challenged and profound immune changes found in the milk. The main biochemical changes measured in milk were partially in accord with lipopolysaccharide-induced mastitis, with increased glucose-6-phosphate and lactate dehydrogenase activity or prolonged lactate dehydrogenase, and Glu6P/Glu alterations. Changes also reflect physiological responses to inflammation in the mammary gland, as in the balance between aerobic and anaerobic metabolism (citrate to lactate ratios). Some alterations measured in milk resolved with days after challenge but other remained for above one month, regardless of bacterial clearance. The results suggest that E. coli mastitits can be divided into two stages: an acute, clinical phase, as an immediate response to bacterial infection in the mammary gland and a chronic phase, independent of bacteria clearance, and in response to the tissue damage caused in the first phase.
How does customer participation in service influence customer satisfaction? The mediating effects of role stressors
Authors: Jiang Yiran , Xu Lan , Cui Nan , Zhang Hui , Yang Zhilin 
Published: April 2019

PurposeThe purpose of this paper is to examine the impact of customer participation on role behaviors and customer satisfaction. The mediating role of role stressors is also examined.Design/methodology/approachBased on literature reviews, a survey of 317 bank customers was conducted in Central China, using a structured questionnaire. Structural equation modeling was used for data analysis to test research hypotheses.FindingsThe current work found that the inconsistency between the role expectations from participating customers and service providers would increase the customer perceived role stress. Therefore, customer participating width and depth can affect customer satisfaction in two different ways. On the one hand, role stressors (i.e. role ambiguity and role conflict) in customer participation have a negative effect on customer compliance, decreasing customer satisfaction. On the other hand, role stressors have a positive effect on customer creativity, increasing customer satisfaction.Originality/valueNo prior studies, thus far, have examined how customer perceived role stressors in service participation affect customers’ role performance and satisfaction in the service process. The current research identifies the characteristics of customer participation from the perspectives of task role set. On the basis of role stressor theory, this research examines the effects of customer participation width and depth on customer satisfaction using customer perceived role stressors as mediating variables. This research also investigates the mixed effect of role stressors on customer satisfaction. It provides empirical support for the role of customers as “co-creators” by distinguishing customers’ creative behaviors from customer compliance and finds the positive effect of role stressors on customer satisfaction via customer creativity.
Interpretive research
Authors: Darby Jessica L. , Fugate Brian S. , Murray Jeff B. 
Published: April 2019

PurposeScholars have called for diversity in methods and multi-method research to enhance relevance to practice. However, many of the calls have only gone so far as to suggest the use of multiple methods within the positivism paradigm, which dominates the discipline and may constrain the ability to develop middle-range theory and propose workable solutions to today’s supply chain challenges. The purpose of this paper is to present a rationale for expanding the methodological toolbox of the field to include interpretive research methods.Design/methodology/approachThis research conceptually illustrates how positivist and interpretive philosophies translate into different research approaches by reviewing an extant positivist qualitative study that uses grounded theory and then detailing how an interpretive researcher would approach the same phenomenon using the hermeneutic method.FindingsThis research expands the boundaries and impact of the field by broadening the set of questions research can address. It contributes a detailed illustration of the interpretive research process, as well as applications for the interpretive approach in future research, particularly theory elaboration, middle-range theorizing, and emerging domains such as the farm-to-fork supply chain and the consumer-based supply chain.Research limitations/implicationsThe development of alternative ways of seeking knowledge enhances the potential for creativity, expansion, and progress in the field.Practical implicationsPractical implications of this research include enabling researchers to elaborate theory and develop middle-range theories through an alternative philosophical paradigm. This paradigm facilitates practical insights that are directly relevant to particular domains and move beyond general theories seeking generalizability.Social implicationsSocial implications of this research are much more indirect in nature. This research encourages supply chain management (SCM) scholars to look at phenomena (including those with social implications) from a different philosophical perspective, which can reveal new insights.Originality/valueThis research contributes a rationale for expanding the methodological toolbox of the field to include interpretive research methods and also contributes a methodological operationalization of the interpretive approach. By reflecting on the nature of science and method in SCM, the study opens the door for creativity and progress to expand the boundaries and impact of the field.
The relationship between social presence and cognitive load
Authors: Costley Jamie 
Published: April 2019

Purpose
As online learning has become more prevalent, how learners interact with each other in those learning environments has become more salient. To develop effective levels of interaction, students must feel comfortable to express their ideas and views. For this reason, this paper aims to look at how individual students’ levels of social presence affect germane cognitive load. Germane cognitive load is the amount that students are able to construct schema and can be seen as analogous to learning.

Design/methodology/approach
This study looks at the results of survey data (n = 433) that investigate the relationship between social presence and germane cognitive load. The students were surveyed from the Open Cyber University of Korea in the fall semester of 2018.

Findings
The present study found a statistically significant positive relationship between social presence and germane cognitive load. The study found a Spearman’s correlation coefficient of 0.595. Furthermore, the sample was divided into a high, medium and low grouping of social presence. Among these groupings, the high level social presence had the highest level of germane cognitive load, and the low level social presence had the lowest level of germane cognitive load.

Originality/value
This result shows the importance and value of developing levels of social presence in online environments. Some research has shown relationships between student interaction and learning, but the present study looks directly at social presence and germane cognitive load. From this research, the authors can see the value of encouraging higher levels of social interaction in online learning environments.
Using the ARDL-ECM approach to investigate the nexus between support price and wheat production
Authors: Chandio Abbas Ali , Jiang Yuansheng , Rehman Abdul 
Published: April 2019

PurposeThe purpose of this paper is to examine the effect of support price on wheat production in Pakistan during the period 1971–2016.Design/methodology/approachTo capture the effect of support price on wheat production, the authors estimated the long-run linkage by using the ARDL bounds testing approach to cointegration.FindingsThis study confirmed the presence of a positive and long-term effect of area under cultivation, support price and fertilizer consumption on wheat production through ARDL bounds test. The results showed that both in the long run and short run, support price plays an important role in the enhancement of wheat production. The authors also found that the coefficients of the area under cultivation and fertilizer consumption variables were statistically significant and positive both in the long run and short run.Originality/valueThe use of the ARDL approach that examines the long-run and short-run effects of support price on wheat production in Pakistan makes the current study unique. An emerging economic literature suggests that only limited research has been conducted in this area.
Strategy beyond the business unit level: corporate parenting in focus
Authors: Galpin Timothy 
Published: April 2019

Purpose
The gap between management theory and practice has been much criticized. To help bridge the divide, a synthesis of empirical, theoretical and practice literature is offered, along with an application of the widely used VRIO framework, to contend that developing a focused corporate parenting approach as a core competence serves as a source of competitive advantage for diversified companies.

Design/methodology/approach
A synthesis of empirical, theoretical and practice literature is presented, beginning with a discussion of why and how firms diversify; the relative performance of firms that pursue related and unrelated diversification; an application of the resource-based view, core competencies and the VRIO framework; a description of focused corporate parenting as a core competency; a prescription for how diversified firms can implement a focused corporate parenting approach; and implications for research.

Findings
Developing a focused corporate parenting approach as a core competence serves as a source of competitive advantage for diversified companies.

Research limitations/implications
The synthesis of empirical, theoretical and practice literature presented provides a foundation for future research into the impact of focused corporate parenting on diversified firm performance.

Practical implications
The paper includes a prescription for how diversified firms can implement a focused corporate parenting approach.

Originality/value
The application of the resource-based view and core competency theories to corporate parenting provides managers with the rationale for and methodology to focus their corporate parenting activities.
The Bayer–Monsanto fusion: countering brand tarnishing and rebuilding reputation
Authors: Rebière Philippe , Mavoori Hareesh 
Published: April 2019

PurposeThe purpose of this paper is to investigate the antecedents, internal/external drivers and circumstances that diminish the importance of reputation and permit it to be circumvented in the context of a fusion (Monsanto-Bayer) and propose a comprehensive stakeholder perception-molding (“sense-giving”) model to counter reputation-damaging factors.Design/methodology/approachThe present study is a longitudinal case study of merging entities using an intra-organizational evolutionary perspective combined with external analysis.FindingsReputational hurdles can be successfully circumvented by a multifaceted strategy leveraging timely and tailored combinations of cognitive, conative and linguistic perception-molding strategies for effective management of diverse stakeholder perception processes spanning across identity orientation, legitimacy, posture, consistency, commitment, justification and transparency.Research limitations/implicationsThe research is susceptible to the general limitations of case studies such as omission bias in terms of focusing on only two purposefully chosen market-leader firms involved in a merger, though every effort was made to track competitor movements, broad trends in markets and the micro- and macro-environments.Practical implicationsThe proposed heptagonal reputation circumvention perception-molding framework (Figure 1) summarizes various actionable strategies to help managers develop a global vision and portfolio of strategies to proactively or reactively manage attacks on reputation.Originality/valueAn in-depth and multi-decade study of antecedents and internal/external drivers of the successful mega-fusion of two companies with a long history of reputational shocks was leveraged to provide unique insights into the interplay of various strategies targeting diverse stakeholder perceptions. These insights were then generalized to create a comprehensive perception-molding strategic framework to help firm managers circumvent reputational tarnishment hurdles.
“It would be pretty immoral to choose a random algorithm”
Authors: Webb Helena , Patel Menisha , Rovatsos Michael , Davoust Alan , Ceppi Sofia , Koene Ansgar , Dowthwaite Liz , Portillo Virginia , Jirotka Marina , Cano Monica 
Published: April 2019

Purpose
The purpose of this paper is to report on empirical work conducted to open up algorithmic interpretability and transparency. In recent years, significant concerns have arisen regarding the increasing pervasiveness of algorithms and the impact of automated decision-making in our lives. Particularly problematic is the lack of transparency surrounding the development of these algorithmic systems and their use. It is often suggested that to make algorithms more fair, they should be made more transparent, but exactly how this can be achieved remains unclear.

Design/methodology/approach
An empirical study was conducted to begin unpacking issues around algorithmic interpretability and transparency. The study involved discussion-based experiments centred around a limited resource allocation scenario which required participants to select their most and least preferred algorithms in a particular context. In addition to collecting quantitative data about preferences, qualitative data captured participants’ expressed reasoning behind their selections.

Findings
Even when provided with the same information about the scenario, participants made different algorithm preference selections and rationalised their selections differently. The study results revealed diversity in participant responses but consistency in the emphasis they placed on normative concerns and the importance of context when accounting for their selections. The issues raised by participants as important to their selections resonate closely with values that have come to the fore in current debates over algorithm prevalence.

Originality/value
This work developed a novel empirical approach that demonstrates the value in pursuing algorithmic interpretability and transparency while also highlighting the complexities surrounding their accomplishment.
Forsake me not: balcony spaces in codes and cues among on-campus apartment dwellers
Authors: Subasinghe Chamila 
Published: April 2019

Purpose
While some people are mindful of what a personal space that also belongs to a common façade portrays to outsiders, why other people treat this personal space as a mere utility space invisible to the public eye must be determined. International students who live in single-bedroom apartments with balconies and were mostly married were investigated regarding the meaning they attach to their balcony spaces. The paper aims to discuss this issue.

Design/methodology/approach
This work also hypothesized that residents of these units perceived their balconies as a liminal space that oscillates between a spatial repertoire for familiar memories and a versatile, utilitarian device for temporary storage. A naturalistic inquiry was then conducted among purposefully sampled apartment dwellers via in-depth, open-ended and semi-structured interviews.

Findings
While offering much needed spatial economy to dwellers, the cues and codes revealed that the balcony space could furnish a sense of membership to established social cohorts. The balcony space further brings an element of escape and ease into impecunious student life by means of its ability to offer a broad spectrum of spatial-aspatial needs that manifested in forms of personalizations and exploitations.

Originality/value
A knowledge gap in socio-cultural appropriation of on-campus apartments for sustainable redevelopment where the majority of consumers were married/partnered, international students has been investigated.