bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spirituality: What Does it Mean and to Whom?
Authors: Steensland Brian , Wang Xiaoyun , Schmidt Lauren Chism 
Published: August 2018

AbstractWhile there is increasing interest in the topic of spirituality, scholars have limited data on its meaning among ordinary Americans. Based on an open‐ended question in a new nationally representative survey, this article documents the elements that make up people's views of spirituality. We find that theism is the dominant focus of American spirituality, with a relatively small percentage of people offering exclusively immanent descriptions. Cognitive and relational orientations are more prominent than behavioral or ethical orientations. Using latent class analysis, we identify seven distinctive views of spirituality that vary considerably in their prevalence and social profiles. Binary logit regression shows that spiritual self‐identification, belief in God, and worship attendance are the religious factors most strongly associated with views of spirituality. Among sociodemographic predictors, significant associations with gender, race, education, and income are limited or absent. In contrast, the influences of age and political ideology are more substantial.
Gall bladder mucoceles in Border terriers
Authors: Allerton F. , Swinbourne F. , Barker L. , Black V. , Kathrani A. , Tivers M. , Henriques T. , Kisielewicz C. , Dunning M. , Kent A. 
Published: August 2018

BackgroundGall bladder mucoceles (GBM) are a leading cause of biliary disease in dogs with several breeds, including the Shetland Sheepdog, American Cocker Spaniel, Chihuahua, Pomeranian, and Miniature Schnauzer apparently predisposed.ObjectiveTo determine risk factors, clinical features, and response to treatment of GBM in Border terriers (BT).AnimalsMedical records of 99 dogs (including 51 BT) with an ultrasonographic (±histopathologic) diagnosis of GBM from three referral centers in the United Kingdom were collected. A control group of 87 similar‐aged BT with no ultrasonographic evidence of gall bladder disease was selected for comparison.MethodRetrospective case‐control study. Odds ratios were calculated to establish breed predisposition. Signalment, presence of endocrine disease, clinicopathologic results, and outcome were compared between the BT, other breeds, and control BTs.ResultsThe odds of identifying a GBM in a BT in this hospital population was 85 times that of all other breeds (95% confidence interval 56.9‐126.8). BT had similar clinical signs and clinicopathologic changes to other breeds with GBM. There was no evidence that endocrinopathies were associated with GBM in BT.Clinical SignificanceA robust breed predisposition to GBM is established for the BT.
Relationship of the mucosal microbiota to gastrointestinal inflammation and small cell intestinal lymphoma in cats
Authors: Garraway Kayode , Johannes Chad M. , Bryan Angela , Peauroi John , Rossi Giacomo , Zhang Min , Wang Chong , Allenspach Karin , Jergens Albert E. 
Published: August 2018

BackgroundThe gastrointestinal (GI) microbiota in healthy cats is altered in IBD. Little research has been performed to identify whether specific bacterial groups are associated with small cell GI lymphoma (LSA).HypothesisMucosal bacteria, including Enterobacteriaceae and Fusobacterium spp., are abundant in intestinal biopsies of cats with small cell GI LSA compared to cats with IBD.AnimalsFourteen cats with IBD and 14 cats with small cell GI LSA.MethodsRetrospective case control study. A search of the medical records was performed to identify cats diagnosed with IBD and with GI LSA. Bacterial groups identified by FISH in GI biopsies were compared between cohorts and correlated to CD11b+ and NF‐κB expression.Results
Fusobacterium spp. (median; IQR bacteria/region) were higher in cats with small cell GI LSA in ileal (527; 455.5 – 661.5; P = .046) and colonic (404.5; 328.8 – 455.5; P = .016) adherent mucus, and combined colonic compartments (free mucus, adherent mucus, attaching to epithelium) (8; 0 – 336; P = .017) compared to cats with IBD (ileum: 67; 31.5 – 259; colon: 142.5; 82.3 – 434.5; combined: 3; 0 – 34). Bacteroides spp. were higher in ileal adherent mucus (P = .036) and 3 combined ileal compartments (P = .034) of cats with small cell GI LSA. There were significant correlations between Fusobacterium spp. totals and CD11b+ cell (P = .009; rs .476) and NF‐κB expression (P = .004; rs .523).ConclusionsThe bacterial alterations appreciated might be influential in development of small cell GI LSA, and should drive further studies to elucidate the effects of microbial‐mediated inflammation on GI cancer progression.
The pharmacokinetics of orally administered butylscopolamine in greyhound dogs
Authors: Morris Tim , Paine Stuart W. , Viljanto Marjaana , Hudson Simon , Gower Simon , Pittorino Melissa , Colgan Sally 
Published: August 2018

AbstractThe oral tablet formulation of butylscopolamine, which is available without prescription, is commonly used by trainers of racing greyhounds to treat functional urethral obstruction. As medication control of butylscopolamine is therefore required for such use to ensure the integrity of greyhound racing, an administration study was performed in six greyhounds to determine the pharmacokinetics of orally administered butylscopolamine. A single dose of one 10 mg butylscopolamine tablet was administered orally to simulate this use in greyhound racing. Blood, urine and faeces were collected at regular intervals from the greyhounds for up to 9 days and butylscopolamine concentrations determined. There was some, but very limited, absorption of butylscopolamine, with rapid elimination from plasma with a mean half‐life of 2 hr. Urine concentrations initially declined in a similar manner to the plasma pharmacokinetics but then entered a much longer half‐life of approximately 50 hr. Faecal concentrations declined to very low levels between 48 and 120 hr. The use of orally administered butylscopolamine for functional urethral obstruction in greyhounds is unjustified due to this very limited drug absorption. Medication control of butylscopolamine's antispasmodic effect on the digestive tract is possible by setting screening limits based on the urinary and faecal drug levels as determined in this study.
Static hedging and pricing of exotic options with payoff frames
Authors: Kirkby Justin Lars , Deng Shijie 
Published: August 2018

AbstractWe develop a general framework for statically hedging and pricing European‐style options with nonstandard terminal payoffs, which can be applied to mixed static–dynamic and semistatic hedges for many path‐dependent exotic options including variance swaps and barrier options. The goal is achieved by separating the hedging and pricing problems to obtain replicating strategies. Once prices have been obtained for a set of basis payoffs, the pricing and hedging of financial securities with arbitrary payoff functions is accomplished by computing a set of “hedge coefficients” for that security. This method is particularly well suited for pricing baskets of options simultaneously, and is robust to discontinuities of payoffs. In addition, the method enables a systematic comparison of the value of a payoff (or portfolio) across a set of competing model specifications with implications for security design.
On the path to extinction: Inbreeding and admixture in a declining grey wolf population
Authors: Gómez‐Sánchez Daniel , Olalde Iñigo , Sastre Natalia , Enseñat Conrad , Carrasco Rafael , Marques‐Bonet Tomas , Lalueza‐Fox Carles , Leonard Jennifer A. , Vilà Carles , Ramírez Oscar 
Published: August 2018

AbstractAllee effects reduce the viability of small populations in many different ways, which act synergistically to lead populations towards extinction vortexes. The Sierra Morena wolf population, isolated in the south of the Iberian Peninsula and composed of just one or few packs for decades, represents a good example of how diverse threats act additively in very small populations. We sequenced the genome of one of the last wolves identified (and road‐killed) in Sierra Morena and that of another wolf in the Iberian Wolf Captive Breeding Program and compared them with other wolf and dog genomes from around the world (including two previously published genome sequences from northern Iberian wolves). The results showed relatively low overall genetic diversity in Iberian wolves, but diverse population histories including past introgression of dog genes. The Sierra Morena wolf had an extraordinarily high level of inbreeding and long runs of homozygosity, resulting from the long isolation. In addition, about one‐third of the genome was of dog origin. Despite the introgression of dog genes, heterozygosity remained low because of continued inbreeding after several hybridization events. The results thus illustrate the case of a small and isolated wolf population where the low population density may have favoured hybridization and introgression of dog alleles, but continued inbreeding may have resulted in large chromosomal fragments of wolf origin completely disappearing from the population, and being replaced by chromosomal fragments of dog origin. The latest population surveys suggest that this population may have gone extinct.
Haemoplasmas in wild rodents: Routes of transmission and infection dynamics
Authors: Cohen Carmit , Shemesh Merav , Garrido Mario , Messika Irit , Einav Monica , Khokhlova Irina , Tasker Séverine , Hawlena Hadas 
Published: August 2018

AbstractThe way that some parasites and pathogens persist in the hostile environment of their host for long periods remains to be resolved. Here, longitudinal field surveys were combined with laboratory experiments to investigate the routes of transmission and infection dynamics of such a pathogen—a wild rodent haemotropic bacterium, specifically a Mycoplasma haemomuris‐like bacterium. Fleaborne transmission, direct rodent‐to‐rodent transmission and vertical transmission from fleas or rodents to their offspring were experimentally quantified, and indications were found that the main route of bacterial transmission is direct, although its rate of successful transmission is low (~20%). The bacterium's temporal dynamics was then compared in the field to that observed under a controlled infection experiment in field‐infected and laboratory‐infected rodents, and indications were found, under all conditions, that the bacterium reached its peak infection level after 25–45 days and then decreased to low bacterial loads, which persist for the rodent's lifetime. These findings suggest that the bacterium relies on persistency with low bacterial loads for long‐term coexistence with its rodent host, having both conceptual and applied implications.
Seasonally varying marine influences on the coastal ecosystem detected through molecular gut analysis
Authors: Verschut Vasiliki , Strandmark Alma , Esparza‐Salas Rodrigo , Hambäck Peter A. 
Published: August 2018

AbstractTerrestrial predators on marine shores benefit from the inflow of organisms and matter from the marine ecosystem, often causing very high predator densities and indirectly affecting the abundance of other prey species on shores. This indirect effect may be particularly strong if predators shift diets between seasons. We therefore quantified the seasonal variation in diet of two wolf spider species that dominate the shoreline predator community, using molecular gut content analyses with general primers to detect the full prey range. Across the season, spider diets changed, with predominantly terrestrial prey from May until July and predominantly marine prey (mainly chironomids) from August until October. This pattern coincided with a change in the spider age and size structure, and prey abundance data and resource selection analyses suggest that the higher consumption of chironomids during autumn is due to an ontogenetic diet shift rather than to variation in prey abundance. The analyses suggested that small dipterans with a weak flight capacity, such as Chironomidae, Sphaeroceridae, Scatopsidae and Ephydridae, were overrepresented in the gut of small juvenile spiders during autumn, whereas larger, more robust prey, such as Lepidoptera, Anthomyidae and Dolichopodidae, were overrepresented in the diet of adult spiders during spring. The effect of the inflow may be that the survival and growth of juvenile spiders is higher in areas with high chironomid abundances, leading to higher densities of adult spiders and higher predation rates on the terrestrial prey next spring.
Reconstruction of female heterogamety from admixture of XX‐XY and ZZ‐ZW sex‐chromosome systems within a frog species
Authors: Ogata Mitsuaki , Lambert Max , Ezaz Tariq , Miura Ikuo 
Published: August 2018

AbstractSex‐determining mechanisms change repeatedly throughout evolution, and it is difficult to track this continual process. The Japanese soil‐frog Glandirana rugosa is a remarkable evolutionary witness to the ongoing process of the evolution of sex‐determining modes. The two geographic groups, designated XY and Neo‐ZW, have homologous sex chromosomes, yet display opposite types of sex chromosomes, XX‐XY and ZZ‐ZW, respectively. These two groups are sympatric at the edges of their respective ranges in Central Japan. In this study, we discovered molecular evidence that the eastern part of the Neo‐ZW group (Neo‐ZW2 subgroup), which is found near the sympatric area, shares mitochondrial haplotypes with the XY group. By analysing single nucleotide polymorphism (SNP) loci, we have also discovered that the representative nuclear genome of the Neo‐ZW2 subgroup shares allele clusters with both the XY group and another part of the Neo‐ZW group (Neo‐ZW1 subgroup), indicating a hybrid origin of the Neo‐ZW2. Further analysis of sex‐linked SNP loci revealed that the alleles on the W chromosomes of the Neo‐ZW2 were derived mostly from X chromosomes, while alleles on the Z chromosomes originated from the Z chromosomes of the Neo‐ZW1 subgroup and partly from the Y chromosomes of the XY group. Our study revealed that admixture of the two opposite sex‐chromosome systems reconstructed a female heterogametic system by recycling the X chromosomes into new W chromosomes. This work offers an illustrative example of how de novo sex‐chromosome systems can arise by recycling material from ancestral sex chromosomes.
A two‐way road: novel roles for fungal extracellular vesicles
Authors: Rodrigues Marcio L. , Casadevall Arturo 
Published: August 2018

SummaryThe biological functions of fungal extracellular vesicles (EVs) or exosomes have been mostly determined on the basis of the assumption that they are vehicles of trans‐cell wall transport and molecular export. The possibility that fungal cells can bind to and internalize EVs remained largely unaddressed. Recent studies, however, demonstrated that fungal cells can internalize host‐derived and/or fungal EVs through processes that profoundly modify their regular physiology. To illustrate this novel view, we discuss (i) the uptake of plant EVs by phytopathogenic fungi culminating in growth defects and virulence attenuation, (ii) the influence of EV internalization in prion transmission and biofilm formation in yeast cells, and (iii) the EV‐mediated transfer of virulence in isolates of Cryptococcus gattii. These recent observations indicate that the functions exerted by EVs in fungal cells result from previously unknown mechanisms of bidirectional transport, opening new venues for the investigation of how EVs impact fungal physiology.
Transformation by growth onto agro‐infiltrated tissues (TGAT), a simple and efficient alternative for transient transformation of the cucurbit powdery mildew pathogen Podosphaera xanthii
Authors: Martínez‐Cruz Jesús , Romero Diego , De Vicente Antonio , Pérez‐García Alejandro 
Published: August 2018

SummaryA major limitation of molecular studies in powdery mildew fungi (Erysiphales) is their genetic intractability. This is because they are obligate biotrophs. In these parasites, biotrophy is determined by the presence of haustoria, which are specialized structures of parasitism that play an essential role in the acquisition of nutrients and the deliverance of effectors. Podosphaera xanthii is the main causal agent of cucurbit powdery mildew and a major limitation for crop productivity. In a previous study using P. xanthii conidia, we showed, for the first time, the transformation of powdery mildew fungi by Agrobacterium tumefaciens. In this work, we hypothesized that the haustorium could also act as a natural route for the acquisition of DNA. To test our hypothesis, melon cotyledons were agro‐infiltrated with A. tumefaciens that contained diverse transfer DNA (T‐DNA) constructs harbouring different marker genes under the control of fungal promoters and, after elimination of the bacterium, the cotyledons were subsequently inoculated with P. xanthii conidia. Our results conclusively demonstrated the transfer of different T‐DNAs from A. tumefaciens to P. xanthii, including two fungicide resistance markers (hph and tub2), a reporter gene (gfp) and a translational fusion (cfp‐PxEC2). These results were further supported by the co‐localization of translational fluorescent fusions of A. tumefaciens VirD2 and P. xanthii Rab5 proteins into small vesicles of haustorial and hyphal cells, suggesting endocytosis as the mechanism for T‐DNA uptake, presumably by the haustorium. From our perspective, transformation by growth onto agro‐infiltrated tissues (TGAT) is the easiest and most reliable method for the transient transformation of powdery mildew fungi.
Novel insights on colonization routes and evolutionary potential of Colletotrichum kahawae, a severe pathogen of Coffea arabica
Authors: Vieira Ana , Silva Diogo Nuno , Várzea Victor , Paulo Octávio Salgueiro , Batista Dora 
Published: August 2018

SummaryPathogenic fungi are emerging at an increasing rate on a wide range of host plants, leading to tremendous threats to the global economy and food safety. Several plant pathogens have been considered to be invasive species, rendering large‐scale population genomic analyses crucial to better understand their demographic history and evolutionary potential. Colletotrichum kahawae (Ck) is a highly aggressive and specialized pathogen, causing coffee berry disease in Arabica coffee in Africa. This pathogen leads to severe production losses and its dissemination out of Africa is greatly feared. To address this issue, a population genomic approach using thousands of single nucleotide polymorphisms (SNPs) spaced throughout the genome was used to unveil its demographic history and evolutionary potential. The current study confirms that Ck is a true clonal pathogen, perfectly adapted to green coffee berries, with three completely differentiated populations (Angolan, Cameroonian and East African). Two independent clonal lineages were found within the Angolan population as opposed to the remaining single clonal populations. The most probable colonization scenario suggests that this pathogen emerged in Angola and immediately dispersed to East Africa, where these two populations began to differentiate, followed by the introduction in Cameroon from an Angolan population. However, the differentiation between the two Angolan clonal lineages masks the mechanism for the emergence of the Cameroonian population. Our results suggest that Ck is completely differentiated from the ancestral lineage, has a low evolutionary potential and a low dispersion ability, with human transport the most likely scenario for its potential dispersion, which makes the fulfilment of the quarantine measures and management practices implemented crucial.
Deciphering the functions of the outer membrane porin OprBXo involved in virulence, motility, exopolysaccharide production, biofilm formation and stress tolerance in Xanthomonas oryzae pv. oryzae
Authors: Bae Nahee , Park Hye‐Jee , Park Hanbi , Kim Minyoung , Han Sang‐Wook 
Published: August 2018

Summary
Xanthomonas oryzae pv. oryzae (Xoo) is a Gram‐negative bacterium causing bacterial leaf blight disease in rice. Previously, proteomic analysis has shown that the outer membrane protein B in Xoo (OprBXo) is more abundant in the wildtype strain than is the outer membrane protein 1 in the Xoo (Omp1X) knockout mutant. OprBXo shows high homology with OprB, which has been well characterized as a carbohydrate‐selective porin in X. citri ssp. citri and Pseudomonas species. However, the functions of OprBXo in Xoo have not yet been documented. To elucidate the functions of OprBXo, we generated the OprBXo‐overexpressing mutant, Xoo(OprBXo), and the knockout mutant, XooΔoprBXo(EV). We found that the virulence and migration of Xoo(OprBXo), but not XooΔoprBXo(EV), were markedly reduced in rice. To postulate the mechanisms affected by OprBXo, comparative proteomic analysis was performed. Based on the results of proteomics, we employed diverse phenotypic assays to characterize the functions of OprBXo. Abnormal twitching motility and reduction in swarming motility were observed in Xoo(OprBXo). Moreover, Xoo(OprBXo) decreased, but XooΔoprBXo(EV) enhanced, exopolysaccharide production and biofilm formation. The chemotactic ability of XooΔoprBXo(EV) was dramatically lower than that of Xoo(EV) in the presence of glucose and xylose. Xoo(OprBXo) was resistant to sodium dodecylsulphate and hydrogen peroxide, but XooΔoprBXo(EV) was highly sensitive compared with Xoo(EV). Thus, OprBXo is not only essential for chemotaxis and stress tolerance, but also for motility, biofilm formation and exopolysaccharide production, which may contribute to the virulence of Xoo. These results will lead to new insights into the functions of a sugar‐selective porin in Xoo.
Impact of genetic drift, selection and accumulation level on virus adaptation to its host plants
Authors: Rousseau Elsa , Tamisier Lucie , Fabre Frederic , Simon Vincent , Szadkowski Marion , Bouchez Olivier , Zanchetta Catherine , Girardot Gregory , Mailleret Ludovic , Grognard Frederic , Palloix Alain , Moury Benoit 
Published: August 2018

SUMMARY
The efficiency of plant major resistance genes is limited by the emergence and spread of resistance‐breaking mutants. Modulation of the evolutionary forces acting on pathogen populations constitutes a promising way to increase the durability of these genes. We studied the effect of four plant traits affecting these evolutionary forces on the rate of resistance breakdown (RB) by a virus. Two of these traits correspond to virus effective population sizes (Ne) at either plant inoculation or during infection. The third trait corresponds to differential selection exerted by the plant on the virus population. Finally, the fourth trait corresponds to within‐plant virus accumulation (VA). These traits were measured experimentally on Potato virus Y (PVY) inoculated to a set of 84 pepper doubled‐haploid lines, all carrying the same pvr23 resistance gene, but having contrasting genetic backgrounds. The lines showed extensive variation for the rate of pvr23 RB by PVY and for the four other traits of interest. A generalized linear model showed that three of these four traits, with the exception of Ne at inoculation, and several pairwise interactions between them had significant effects on RB. RB increased with increasing values of Ne during plant infection or VA. The effect of differential selection was more complex because of a strong interaction with VA. When VA was high, RB increased as the differential selection increased. An opposite relationship between RB and differential selection was observed when VA was low. This study provides a framework to select plants with appropriate virus evolution‐related traits to avoid or delay RB.