bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synchronization in time-varying random networks with vanishing connectivity
Authors: Faggian Marco , Ginelli Francesco , Rosas Fernando , Levnajić Zoran 
Published: July 2019

AbstractA sufficiently connected topology linking the constituent units of a complex system is usually seen as a prerequisite for the emergence of collective phenomena such as synchronization. We present a random network of heterogeneous phase oscillators in which the links mediating the interactions are constantly rearranged with a characteristic timescale and, possibly, an extremely low instantaneous connectivity. We show that with strong coupling and sufficiently fast rewiring the network reaches partial synchronization even in the vanishing connectivity limit. In particular, we provide an approximate analytical argument, based on the comparison between the different characteristic timescales of our system in the low connectivity regime, which is able to predict the transition to synchronization threshold with satisfactory precision beyond the formal fast rewiring limit. We interpret our results as a qualitative mechanism for emergence of consensus in social communities. In particular, our result suggest that groups of individuals are capable of aligning their opinions under extremely sparse exchanges of views, which is reminiscent of fast communications that take place in the modern social media. Our results may also be relevant to characterize the onset of collective behavior in engineered systems of mobile units with limited wireless capabilities.
The Effect of Electronic Cigarette User Modifications and E-liquid Adulteration on the Particle Size Profile of an Aerosolized Product
Authors: Mulder Haley A. , Patterson Jesse L. , Halquist Matthew S. , Kosmider Leon , Turner Joseph B. McGee , Poklis Justin L , Poklis Alphonse , Peace Michelle R. 
Published: July 2019

AbstractElectronic cigarettes (e-cigarettes) are an alternate nicotine delivery system that generate a condensation aerosol to be inhaled by the user. The size of the droplets formed in the aerosol can vary and contributes to drug deposition and ultimate bioavailability in the lung. The growing popularity of e-cigarette products has caused an increase in internet sources promoting the use of drugs other than nicotine (DOTNs) in e-cigarettes. The purpose of this study was to determine the effect of various e-cigarette and e-liquid modifications, such as coil resistance, battery voltage, and glycol and drug formulation, on the aerosol particle size. E-liquids containing 12 mg/mL nicotine prepared in glycol compositions of 100% propylene glycol (PG), 100% vegetable glycerin (VG), or 50:50 PG:VG were aerosolized at three voltages and three coil resistances. Methamphetamine and methadone e-liquids were prepared at 60 mg/mL in 50:50 PG:VG and all e-liquids were aerosolized onto a 10 stage Micro-Orifice Uniform Deposit Impactor. Glycol deposition correlated with drug deposition, and the majority of particles centered between 0.172–0.5 μm in diameter, representing pulmonary deposition. The 100% PG e-liquid produced the largest aerosol particles and the 100% VG and 50:50 PG:VG e-liquids produced ultra-fine particles <0.3 μm. The presence of ultrafine particles indicates that drugs can be aerosolized and reach the pulmonary alveolar regions, highlighting a potential for abuse and risk of overdose with DOTNs aerosolized in an e-cigarette system.
Experimental study on flow characteristics of gas transport in micro- and nanoscale pores
Authors: Shen Weijun , Song Fuquan , Hu Xiao , Zhu Genmin , Zhu Weiyao 
Published: July 2019

AbstractGas flow behavior in porous media with micro- and nanoscale pores has always been attracted great attention. Gas transport mechanism in such pores is a complex problem, which includes continuous flow, slip flow and transition flow. In this study, the microtubes of quartz microcapillary and nanopores alumina membrane were used, and the gas flow measurements through the microtubes and nanopores with the diameters ranging from 6.42 μm to 12.5 nm were conducted. The experimental results show that the gas flow characteristics are in rough agreement with the Hagen-Poiseuille (H-P) equation in microscale. However, the flux of gas flow through the nanopores is larger than the H-P equation by more than an order of magnitude, and thus the H-P equation considerably underestimates gas flux. The Knudsen diffusion and slip flow coexist in the nanoscale pores and their contributions to the gas flux increase as the diameter decreases. The slip flow increases with the decrease in diameter, and the slip length decreases with the increase in driving pressure. Furthermore, the experimental gas flow resistance is less than the theoretical value in the nanopores and the flow resistance decreases along with the decrease in diameter, which explains the phenomenon of flux increase and the occurrence of a considerable slip length in nanoscale. These results can provide insights into a better understanding of gas flow in micro- and nanoscale pores and enable us to exactly predict and actively control gas slip.
Metasurface Antennas: New Models, Applications and Realizations
Authors: Faenzi Marco , Minatti Gabriele , González-Ovejero David , Caminita Francesco , Martini Enrica , Della Giovampaola Cristian , Maci Stefano 
Published: July 2019

AbstractThis paper presents new designs, implementation and experiments of metasurface (MTS) antennas constituted by subwavelength elements printed on a grounded dielectric slab. These antennas exploit the interaction between a cylindrical surface wave (SW) wavefront and an anisotropic impedance boundary condition (BC) to produce an almost arbitrary aperture field. They are extremely thin and excited by a simple in-plane monopole. By tailoring the BC through the shaping of the printed elements, these antennas can be largely customized in terms of beam shape, bandwidth and polarization. In this paper, we describe new designs and their implementation and measurements. It is experimentally shown for the first time that these antennas can have aperture efficiency up to 70%, a bandwidth up to 30%, they can produce two different direction beams of high-gain and similar beams at two different frequencies, showing performances never reached before.
Phosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand
Authors: Adams Nathan B. P. , Robertson Angus J. , Hunter C. Neil , Hitchcock Andrew , Bisson Claudine 
Published: July 2019

AbstractPhosphorus acquisition is critical for life. In low phosphate conditions, some species of bacteria have evolved mechanisms to import reduced phosphorus compounds, such as phosphite and hypophosphite, as alternative phosphorus sources. Uptake is facilitated by high-affinity periplasmic binding proteins (PBPs) that bind cargo in the periplasm and shuttle it to an ATP-binding cassette (ABC)-transporter in the bacterial inner membrane. PtxB and HtxB are the PBPs responsible for binding phosphite and hypophosphite, respectively. They recognize the P-H bond of phosphite/hypophosphite via a conserved P-H...π interaction, which confers nanomolar dissociation constants for their respective ligands. PtxB also has a low-level binding affinity for phosphate and hypophosphite, whilst HtxB can facilitate phosphite uptake in vivo. However, HtxB does not bind phosphate, thus the HtxBCDE transporter has recently been successfully exploited for biocontainment of genetically modified organisms by phosphite-dependent growth. Here we use a combination of X-ray crystallography, NMR and Microscale Thermophoresis to show that phosphite binding to HtxB depends on the protonation state of the ligand, suggesting that pH may effect the efficiency of phosphite uptake by HtxB in biotechnology applications.
Loss of Cubilin, the intrinsic factor-vitamin B12 receptor, impairs visceral endoderm endocytosis and endodermal patterning in the mouse
Authors: Perea-Gomez Aitana , Cases Olivier , Lelièvre Vincent , Pulina Maria V. , Collignon Jérôme , Hadjantonakis Anna-Katerina , Kozyraki Renata 
Published: July 2019

AbstractThe visceral endoderm is a polarized epithelial monolayer necessary for early embryonic development in rodents. A key feature of this epithelium is an active endocytosis and degradation of maternal nutrients, in addition to being the source of various signaling molecules or inhibitors required for the differentiation and patterning of adjacent embryonic tissues. Endocytosis across the visceral endoderm epithelium involves specific cell surface receptors and an extensive sub-membrane vesicular system with numerous apical vacuoles/lysosomes. We previously reported that Cubilin, the endocytic receptor for intrinsic factor-vitamin B12, albumin and apolipoproteinA-I/HDL allows maternal nutrient uptake by the visceral endoderm. In the present study, we show that the germline ablation of Cubilin impairs endodermal and mesodermal patterning, and results in developmental arrest at gastrulation. Notably, visceral endoderm dispersal is impeded in Cubilin null embryos. We further confirm the essential role of Cubilin in nutrient internalization by the early visceral endoderm and highlight its involvement in the formation of apical vacuoles. Our results reveal essential roles for Cubilin in early embryonic development, and suggest that in addition to its nutritive function, Cubilin sustains signaling pathways involved in embryonic differentiation and patterning.
Peripartum depression and infant care, sleep and growth
Authors: Iwata Sachiko , Kinoshita Masahiro , Fujita Fumie , Tsuda Kennosuke , Unno Mitsuaki , Horinouchi Takashi , Morokuma Seiichi , Saitoh Shinji , Iwata Osuke 
Published: July 2019

AbstractPeripartum depression is a common, serious complication in mothers. To assess the influence of infant care, sleep and growth on the risk of peripartum depression, 1,271 mothers of healthy one-month-old infants completed comprehensive questionnaires including the Edinburgh Postnatal Depression Scale. Relationships between high depression scores and variables related to infants’ care, sleep and growth were assessed adjusting for other variables. High depression scores were found in 233 mothers, which were associated with variables related to infants’ care (poor satisfaction with infant care support, p < 0.001; mothers’ passive response to night-time fussing, p = 0.001), sleep (dim bedroom lighting, p < 0.05; short sleep duration, p < 0.05) and growth (poor weight gain, p < 0.05), as well as maternal variables (primiparas, p < 0.001; poor income satisfaction, p < 0.001; poor sleep satisfaction, p < 0.001; daytime sleepiness, p = 0.001). Considering the observed association between high depression scores and infants’ care, sleep and growth, a multidisciplinary approach accounting for infant care would be required to prevent peripartum depression.
Intrabody-based FRET probe to visualize endogenous histone acetylation
Authors: Chung Chan-I , Sato Yuko , Ohmuro-Matsuyama Yuki , Machida Shinichi , Kurumizaka Hitoshi , Kimura Hiroshi , Ueda Hiroshi 
Published: July 2019

AbstractPost-translational histone modifications are major regulators of gene expression. However, conventional immunoassays do not provide sufficient information regarding their spatial and temporal dynamic changes. Fluorescence/Förster resonance energy transfer (FRET)-based probes are capable of monitoring the dynamic changes associated with histone modifications in real-time by measuring the balance between histone-modifying enzyme activities. Recently, a genetically encoded histone-modification fluorescent probe using a single-chain variable region (scFv) fragment of a specific antibody was developed. The probe, modification-specific intracellular antibody, is capable of monitoring histone-acetylation levels in both cultured cells and living organisms based on the ratio of fluorescence intensities between the cell nucleus and cytoplasm. In this study, we constructed a FRET probe composed of yellow fluorescent protein attached at the N-terminus of an acetyl H3K9-specific scFv, tethered to a cyan fluorescent protein. When the FRET probe was expressed in human cells, both FRET efficiency and fluorescence intensity in the nucleus increased following histone-deacetylase inhibitor treatment. Using these two parameters, endogenous histone-acetylation levels were quantified over a high dynamic range. This probe provides a simple approach to quantify spatial and temporal dynamic changes in histone acetylation.
Temperature rising would slow down tropical forest dynamic in the Guiana Shield
Authors: Aubry-Kientz Mélaine , Rossi Vivien , Cornu Guillaume , Wagner Fabien , Hérault Bruno 
Published: July 2019

AbstractIncreasing evidence shows that the functioning of the tropical forest biome is intimately related to the climate variability with some variables such as annual precipitation, temperature or seasonal water stress identified as key drivers of ecosystem dynamics. How tropical tree communities will respond to the future climate change is hard to predict primarily because several demographic processes act together to shape the forest ecosystem general behavior. To overcome this limitation, we used a joint individual-based model to simulate, over the next century, a tropical forest community experiencing the climate change expected in the Guiana Shield. The model is climate dependent: temperature, precipitation and water stress are used as predictors of the joint growth and mortality rates. We ran simulations for the next century using predictions of the IPCC 5AR, building three different climate scenarios (optimistic RCP2.6, intermediate, pessimistic RCP8.5) and a control (current climate). The basal area, above-ground fresh biomass, quadratic diameter, tree growth and mortality rates were then computed as summary statistics to characterize the resulting forest ecosystem. Whatever the scenario, all ecosystem process and structure variables exhibited decreasing values as compared to the control. A sensitivity analysis identified the temperature as the strongest climate driver of this behavior, highlighting a possible temperature-driven drop of 40% in average forest growth. This conclusion is alarming, as temperature rises have been consensually predicted by all climate scenarios of the IPCC 5AR. Our study highlights the potential slow-down danger that tropical forests will face in the Guiana Shield during the next century.
Strength enhancement and slip behaviour of high-entropy carbide grains during micro-compression
Authors: Csanádi Tamás , Castle Elinor , Reece Michael J. , Dusza Ján 
Published: July 2019

AbstractBulk polycrystalline high-entropy carbides are a newly developed group of materials that increase the limited compositional space of ultra-high temperature ceramics, which can withstand extreme environments exceeding 2000 °C in oxidizing atmospheres. Since the deformability of grains plays an important role in macromechanical performance, in this work we studied the strength and slip behaviour of grains of a spark-plasma sintered (Hf-Ta-Zr-Nb)C high-entropy carbide in a specific orientation during micropillar compression. For comparison, identical measurements were carried out on the monocarbides HfC and TaC. It was revealed that (Hf-Ta-Zr-Nb)C had a significantly enhanced yield and failure strength compared to the corresponding base monocarbides, while maintaining a similar ductility to the least brittle monocarbide (TaC) during the operation of $${\boldsymbol{\{}}{\bf{110}}{\boldsymbol{\}}}{\boldsymbol{\langle }}{\bf{1}}\bar{{\bf{1}}}{\bf{0}}{\boldsymbol{\rangle }}$$
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slip systems. Additionally, it was concluded that the crystal orientation and stress conditions determine the operation of slip systems in mono- and high-entropy carbides at room temperature.
BK channel clustering is required for normal behavioral alcohol sensitivity in C. elegans
Authors: Oh Kelly H. , Kim Hongkyun 
Published: July 2019

AbstractThe large conductance, calcium- and voltage-activated potassium channel, known as the BK channel, is one of the central proteins that mediate alcohol intoxication and tolerance across species. Although ethanol targets BK channels through direct interaction, how ethanol-mediated BK channel activation causes behavioral intoxication is poorly understood. In. C. elegans, loss of function in SLO-1, the BK channel ortholog, confers profound ethanol resistance in movement and egg-laying behaviors. Here, we show that depletion of SLO-1 channels clustered at the active zones with no change in the overall channel expression level results in locomotory resistance to the intoxicating effect of ethanol, equivalent to that of slo-1 loss-of-function mutants. Likewise, depletion of clustered SLO-1 channels in the sarcolemma and neurons leads to ethanol-resistant egg-laying behavior. By contrast, reduction in the overall SLO-1 channel level by over 70% causes only moderate ethanol resistance in movement, and minimal, if any, resistance in egg laying. Our findings strongly suggest that behavioral ethanol sensitivity is conferred by local, but not global, depression of excitability via clustered BK channels. Given that clustered BK channels are functionally coupled to, and localize near, calcium channels, ethanol may mediate its behavioral effects by targeting BK channels and their coupled calcium channels.
Accumulation of protein aggregates induces autolytic programmed cell death in hybrid tobacco cells expressing hybrid lethality
Authors: Ueno Naoya , Kashiwagi Megumi , Kanekatsu Motoki , Marubashi Wataru , Yamada Tetsuya 
Published: July 2019

AbstractHybrid cells of Nicotiana suaveolens x N. tabacum grow normally at 36 °C, but immediately express lethality due to probable autoimmune response when transferred from 36 to 28 °C. Our recent study showed that the temperature-sensitive lethality of these hybrid cells occurs through autolytic programmed cell death (PCD). However, what happens in hybrid cells following the induction of autoimmune response to autolytic PCD is unclear. We hypothesized that accumulation of protein aggregates in hybrid cells induces autolytic PCD and examined detergent-insoluble protein (protein aggregates) isolated from hybrid cells expressing lethality. The amount of insoluble proteins increased in hybrid cells. Sodium 4-phenylbutyrate, a chemical chaperone, inhibited both the accumulation of insoluble proteins and irreversible progression of cell death. In contrast, E-64, a cysteine protease inhibitor, accelerated both the accumulation of insoluble proteins and cell death. Moreover, proteome analysis revealed that proteasome-component proteins were accumulated specifically in cells treated with E-64, and proteasome activity of hybrid cells decreased after induction of lethality. These findings demonstrate that accumulation of protein aggregates, including proteasome subunits, eventually cause autolytic PCD in hybrid cells. This suggests a novel process inducing plant PCD by loss of protein homeostasis and provides clues to future approaches for elucidating the whole process.
Adversity and cooperation in heterogeneous pairs
Authors: De Jaegher Kris 
Published: July 2019

AbstractThis paper provides a game-theoretic model of the effect of higher adversity on the evolution of cooperation. The focus lies on how this effect of higher adversity is impacted when there is transient, non-genetic heterogeneity in the form of differences in the players’ capabilities of contributing to the public good, in the benefits they obtain from the public good, or in their cooperation costs. A framework is provided that identifies the common mechanisms that are at work across two models of cooperation (jointly producing a public good, and jointly defending an existing public good), and across the mentioned types of heterogeneity. With relatively small heterogeneity, higher adversity generates a common-enemy effect for large cooperation costs and a deterrence effect for small cooperation costs. Yet, these results on the effect of higher adversity are completely reversed for relatively large heterogeneity.
Revealing epilepsy type using a computational analysis of interictal EEG
Authors: Lopes Marinho A. , Perani Suejen , Yaakub Siti N. , Richardson Mark P. , Goodfellow Marc , Terry John R. 
Published: July 2019

AbstractSeizure onset in epilepsy can usually be classified as focal or generalized, based on a combination of clinical phenomenology of the seizures, EEG recordings and MRI. This classification may be challenging when seizures and interictal epileptiform discharges are infrequent or discordant, and MRI does not reveal any apparent abnormalities. To address this challenge, we introduce the concept of Ictogenic Spread (IS) as a prediction of how pathological electrical activity associated with seizures will propagate throughout a brain network. This measure is defined using a person-specific computer representation of the functional network of the brain, constructed from interictal EEG, combined with a computer model of the transition from background to seizure-like activity within nodes of a distributed network. Applying this method to a dataset comprising scalp EEG from 38 people with epilepsy (17 with genetic generalized epilepsy (GGE), 21 with mesial temporal lobe epilepsy (mTLE)), we find that people with GGE display a higher IS in comparison to those with mTLE. We propose IS as a candidate computational biomarker to classify focal and generalized epilepsy using interictal EEG.
Poorly controlled diabetes during pregnancy and lactation activates the Foxo1 pathway and causes glucose intolerance in adult offspring
Authors: Inoguchi Yukihiro , Ichiyanagi Kenji , Ohishi Hiroaki , Maeda Yasutaka , Sonoda Noriyuki , Ogawa Yoshihiro , Inoguchi Toyoshi , Sasaki Hiroyuki 
Published: July 2019

AbstractExposure to maternal diabetes during pregnancy results in diabetes in offspring, but its underlying mechanisms are unclear. Here, we investigated the phenotype and molecular defects of the offspring of poorly controlled diabetic female mice generated by streptozotocin (STZ) administration. Offspring was exposed to maternal diabetes during pregnancy and lactation. The body weight of STZ offspring was lower than that of control offspring at birth and in adulthood, and glucose tolerance was impaired in adult STZ offspring. Interestingly, the phenotype was more pronounced in male offspring. We next investigated the morphology of islets and expression of β cell-related genes, but no significant changes were observed. However, transcriptome analysis of the liver revealed activation of the fork head box protein O1 (Foxo1) pathway in STZ male offspring. Notably, two key gluconeogenesis enzyme genes, glucose 6 phosphatase catalytic subunit (G6pc) and phosphoenolpyruvate carboxykinase 1 (Pck1), were upregulated. Consistent with this finding, phosphorylation of Foxo1 was decreased in the liver of STZ male offspring. These changes were not obvious in female offspring. The activation of Foxo1 and gluconeogenesis in the liver may have contributed to the impaired glucose tolerance of STZ male offspring.