bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying patients likely to be referred for a clinical pharmacist intervention in a family medicine residency‐training clinic
Authors: Vande Griend Joseph P. , Saseen Joseph J. , Sullivan Katherine J. , Anderson Heather D. 
Published: October 2018

IntroductionLittle is known about patient populations within family medicine residency‐training clinics that should be targeted for clinical pharmacy interventions. The objective of this study was to identify patient characteristics associated with receiving an intervention by a clinical pharmacist.MethodsThis retrospective cohort study included 4870 patients aged 40 to 89 years with at least one office visit at the University of Colorado AF Williams Family Medicine clinic from August 1, 2014 to July 31, 2015. Electronic health record data were used to examine associations between 23 previously identified patient‐specific elements and receipt of a clinical pharmacist intervention. Presence of a clinical pharmacist consultation note in the patient's record in the past 12 months was evidence of an intervention. Multivariable logistic regression was used to estimate adjusted odds ratios.ResultsFour percent (n = 182) of patients received at least one clinical pharmacist intervention. Multivariable logistic regression indicated that ≥10 active medications, A1c ≥ 9%, hypertension, depression/anxiety/panic disorder, asthma, indication for anticoagulant therapy, hospitalization/emergency department visit in the last 30 days, age ≥ 65, and Medicaid/uninsured increased the likelihood of receiving a clinical pharmacist intervention, while non‐English speaking significantly decreased the likelihood of receiving a clinical pharmacist intervention. Patients with 7 or more elements were seven times more likely to have a clinical pharmacist intervention than patients with 6 or fewer items (odds ratio = 7.0, 95% confidence interval = 5.2‐9.5).ConclusionsPatients with more elements were more likely to have received a clinical pharmacist intervention. The accumulation of these elements could be used for targeted population‐based clinical pharmacist activities in residency training clinics.
Toxicity of iron oxide nanoparticles: Size and coating effects
Authors: Abakumov Maxim A. , Semkina Alevtina S. , Skorikov Alexander S. , Vishnevskiy Daniil A. , Ivanova Anna V. , Mironova Elena , Davydova Galina A. , Majouga Alexander G. , Chekhonin Vladimir P. 
Published: October 2018

AbstractToxicological research of novel nanomaterials is a major developmental step of their clinical approval. Since iron oxide magnetic nanoparticles have a great potential in cancer treatment and diagnostics, the investigation of their toxic properties is very topical. In this paper we synthesized bovine serum albumin‐coated iron oxide nanoparticles with different sizes and their polyethylene glycol derivative. To prove high biocompatibility of obtained nanoparticles the number of in vitro toxicological tests on human fibroblasts and U251 glioblastoma cells was performed. It was shown that albumin nanoparticles’ coating provides a stable and biocompatible shell and prevents cytotoxicity of magnetite core. On long exposure times (48 hours), cytotoxicity of iron oxide nanoparticles takes place due to free radical production, but this toxic effect may be neutralized by using polyethylene glycol modification.
Autophagy inhibition‐enhanced assembly of the NLRP3 inflammasome is associated with cisplatin‐induced acute injury to the liver and kidneys in rats
Authors: Qu Xiaoyu , Gao Huan , Tao Lina , Zhang Yueming , Zhai Jinghui , Song Yanqing , Zhang Sixi 
Published: October 2018

AbstractThe nucleotide‐binding oligomerization domain‐like receptor family, pyrin domain containing 3 (NLRP3) inflammasome has a key role in the inflammatory response. We found that cisplatin (7.5, 15 mg/kg, IV) could induce acute injury to the liver and kidneys of rats. Western blot and immunohistochemical analyses showed that expression of NLRP3, caspase‐1 and interleukin‐1β was upregulated significantly in a dose‐dependent manner after cisplatin exposure. Autophagy could inhibit NLRP3 expression and assembly of the NLRP3 inflammasome. Expression of light chain 3 II/I and p62 suggested that autophagy was inhibited during injury to the liver and kidneys. These data suggested that cisplatin might activate NLRP3 by inhibiting autophagy in the liver and kidneys of rats.
Thymoquinone attenuates lead‐induced nephropathy in rats
Authors: Mabrouk Aymen 
Published: October 2018

AbstractKidney hazards from lead (Pb) exposure are one of the fastest growing areas of concern in toxicology today. The thymoquinone (TQ) renoprotective effect against Pb‐induced nephropathy has not previously been studied. Therefore, adult male Wistar rats were treated with Pb (2000 ppm of Pb acetate in drinking water) and/or TQ (5 mg/kg/day,
per os). All treatments were applied for 5 weeks. The results indicated that Pb exposure produced metal deposition, histopathological changes, functional impairment (significant elevation in plasma urea, uric acid, and creatinine levels), total antioxidant status decrease, and lipid peroxidation stimulation in the kidneys. Interestingly, TQ supplementation remarkably improved the Pb‐induced renal adverse effects without significantly reducing the tissue metal accumulation. In conclusion, our data indicate for the first time a protective effect of TQ against Pb‐induced nephropathy, most likely through an antioxidant mechanism. On this basis, TQ deserves more consideration and further examination as a potential therapeutic option.
Performance evaluation of five commercial assays for detection of acetaminophen
Authors: Chan Bao‐Yum , Tsang Hing‐Man , Ng Candy Wai‐Yan , Ling William Hin‐Wing , Leung Daniel Cheuk‐Wa , Lee Hencher Han‐Chih , Mak Chloe Miu 
Published: October 2018

BackgroundTo evaluate the analytical performance of five commercial acetaminophen assays and select the best method for routine use.MethodsImprecision, accuracy, linearity, and interferences of three enzymatic assays (Beckman Coulter AU Paracetamol, Abbott MULTIGENT Acetaminophen, and Sekisui Acetaminophen L3K) and two immunoassay‐based assays (Beckman Coulter SYNCHRON ACTM (Acetaminophen) Reagent and Siemens SYVA Emit‐tox Acetaminophen) were evaluated on a Beckman Coulter AU680 chemistry analyzer. Hook effect for immunoassay‐based assays and recovery in ultrafiltrate for enzymatic methods were studied.ResultsWithin‐run and between‐run imprecision of the enzymatic assays ranged 0.26%‐0.82% and 0.53%‐2.86%, respectively, while that for the immunoassay‐based methods ranged 0.96%‐6.34% and 1.50%‐11.33%, respectively. All assays except the SYNCHRON assay fell within the program analytical performance specifications (±20 µmol/L or 10%) for external quality assurance (EQA) samples, with the highest positive bias (31.7%) observed in the SYNCHRON assay. Icteric interference was demonstrated most significantly in the Abbott assay (up to 88 μmol/L positive bias in blank serum). The lipemic interference on the SYNCHRON was significant (up to 110% positive bias at level of 100 μmol/L). The immunoassay‐based methods were less susceptible to hemolytic interference, while the Abbott and AU assays were more susceptible to N‐acetylcysteine interference. Both immunoassay‐based methods showed no hook effect up to 18 000 μmol/L. Ultrafiltration recoveries for enzymatic methods were satisfactory, ranging from 80.0% ± 5.1% to 89.5% ± 3.0%.ConclusionsProportional bias was observed in the SYNCHRON assay, while the Siemens and Sekisui assays were minimally affected by bilirubin interferences.
Pilot evaluation of a targeted intervention for peer‐victimized youth
Authors: Fite Paula J. , Cooley John L. , Poquiz Jonathan , Williford Anne 
Published: October 2018

AbstractObjectiveDue to the limited effectiveness of extant prevention and intervention strategies, the current study is an initial evaluation of a cognitive behavioral group intervention, originally designed to treat symptoms of depression and anxiety, for youth who experienced peer victimization.MethodsTwelve third‐ through fifth‐grade youth participated in the intervention, and their data were compared with 12 youth who were a part of a naturalistic control group. Additionally, school‐wide data are reported to provide overall school trends.ResultsWhereas the intervention group participants exhibited decreases in relational victimization, depressive symptoms, and passive coping, the control group participants exhibited nonsignificant increases in relational victimization, depressive symptoms, and passive coping. School‐wide data also indicated overall increases in relational victimization and depressive symptoms, but no changes in passive coping.ConclusionFindings suggest that cognitive behavioral group interventions may provide a promising avenue for addressing the mental health needs of victimized elementary school‐age youth.
Convergence between Rorschach and self‐report: A new look at some old questions
Authors: Morey Leslie C. , McCredie Morgan N. 
Published: October 2018

AbstractObjectiveThis study presents an examination of the influence of response format on convergence between performance‐based and self‐report assessments of similar mental health constructs, to determine if such method variance might account for prior findings of lack of relationship.MethodsAn online sample of 455 participants (57% male; average age, 35.5) completed a multiple‐choice version of the Rorschach and two self‐report instruments, the Personality Assessment Inventory (PAI) and the International Personality Item Pool (IPIP) representation of the domain traits of the five‐factor model (FFM).ResultsSeveral significant interrelationships emerged between the Rorschach Amplified Multiple Choice Test and the PAI and IPIP five‐factor scales.ConclusionsThese findings suggest that the Rorschach can correlate meaningfully with similar constructs assessed using self‐report methodology when comparable response formats are utilized.
Impact of hydraulic retention time and current on the microbial community and denitrification genes in a continuous‐flow biofilm electrode reactor
Authors: Zhai Siyuan , Ji Min , Pavlostathis Spyros G , Zhao Yingxin 
Published: October 2018

AbstractBACKGROUNDA biofilm‐electrode reactor (BER) was developed on the basis of hydrogenotrophic denitrification. The BER can be strengthened by adding organic matter, and the nitrate removal will be enhanced by a combination of heterotrophic and autotrophic denitrification. Denitrification in a biological system can be affected by operating parameters, and the microbial community and functional genes can be changed at the same time. In this study, the effect of hydraulic retention time (HRT) and current on the denitrification of a low chemical oxygen demand to nitrogen ratio (COD/N), saline wastewater in a continuous‐flow BER was evaluated and compared to a conventional biofilm reactor (BR).RESULTSThe highest extent of nitrate removal was obtained at the optimum HRT (8 h) and current (10 mA). Low levels of ammonia (0.5–4 mg L−1), produced through dissimilatory nitrate reduction to ammonia (DNRA), were observed. Quantitative real‐time polymerase chain reaction analysis revealed that the proportion of nirS‐type denitrifiers (3.75–8.30%) exceeded nirK‐type denitrifiers (0.24–0.90%) in both BR and BER. The genera of T78, Paracoccus and Azoarcus were identified as the dominant denitrifying bacteria in the BER. The cooperative activity of nitrate, nitrite and nitrous oxide reductase is necessary to reduce nitrate to dinitrogen in the denitrification process. The highest ratio of nosZ/nir genes in the bacterial community was 0.25 when the applied current was 10 mA.CONCLUSIONCompared to other denitrification reactors, the BER is more effective to treat low‐COD/N, saline wastewater with short HRT and low current. © 2018 Society of Chemical Industry
Sonographic diagnosis of coexisting ectopic thyroid and fourth branchial cleft cyst
Authors: Oh Jeong Hee , Chang Yun Woo , Lee Eun Ji 
Published: October 2018

AbstractEctopic thyroid and fourth branchial cleft anomaly are rare congenital anomalies of the neck. This is a case report of the coexistence of these two rare congenital anomalies in a 1‐year‐old girl. She had ectopic lingual thyroid and asymptomatic abscess in the fourth branchial cleft cyst, which was found in ultrasonography carried out to evaluate congenital hypothyroidism. To the best of our knowledge, this is the first reported case of ectopic thyroid coexisting with fourth branchial cleft anomaly in the same patient.
Ultrastructural description of fresh and frozen/thawed sperm derived from collared peccaries (Pecari tajacu Linnaeus, 1,758)
Authors: Bezerra Luana Grasiele Pereira , Souza Ana Liza Paz , Silva Herlon Victor Rodrigues , Vasconcelos Fábio Roger , Moura Arlindo de Alencar Araripe , Pereira Alexsandra Fernandes , Oliveira Moacir Franco de , Silva Alexandre Rodrigues 
Published: October 2018

AbstractThe aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen–thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane integrity, membrane functionality, chromatin integrity, and morphology through light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) and glycerol (6%). Then, fresh and frozen–thawed semen samples were mixed in different sperm pools that were processed for SEM and TEM. Sperm motility, membrane integrity, and functionality were impaired (p < .05) by freezing–thawing procedures, but sperm morphology, and chromatin integrity evaluated by light microscopy were not significantly affected. The SEM revealed that peccaries’ sperm presents a flattened head in a paddle format, measuring 6.07 μm in length and 3.84 μm in width, with a vastus acrosome (4.46 μm). Normal tails measure 38.11 μm, being formed by an extensive midpiece with 15.52 μm in length. In frozen–thawed samples, both SEM and TEM provide us information about damage undetected through light microscopy as the presence of vesicles in the acrosome, loose plasma membrane, vacuolized mitochondria, dense fibers disorganized, and decondensed chromatin. In conclusion, we provide the first description of the sperm ultrasctruture in collared peccaries. Moreover, SEM and TEM help us to identify some nanometric damage provoked by freezing–thawing procedures, thus providing valuable information for the improvement of such important protocols used for biobanking formation.
Increased iron deposition of deep cerebral gray matter structures in hemodialysis patients: A longitudinal study using quantitative susceptibility mapping
Authors: Chai Chao , Wang Huiying , Liu Saifeng , Chu Zhi‐Qiang , Li Jinping , Qian Tianyi , Haacke E.M. , Xia Shuang , Shen Wen 
Published: October 2018

BackgroundThe cerebral iron overload in hemodialysis patients has been reported in a previous study, in which the evaluation of the changes in iron content could be affected by the cross‐sectional analysis.PurposeTo investigate the longitudinal changes of iron deposition in hemodialysis patients using quantitative susceptibility mapping (QSM) and correlate these findings with the longitudinal changes of neurocognitive function and clinical factors.Study TypeProspective; longitudinal.PopulationIn all, 34 patients and 30 healthy controls (HCs); the mean follow‐up interval was 22 ± 7 months.Field Strength/Sequence3.0T, susceptibility‐weighted imaging (SWI).AssessmentQSM reconstructed from original phase data of SWI was used to measure the susceptibility of gray matter structures including bilateral caudate nucleus (CN), globus pallidus (GP), putmen (PUT), red nucleus (RN), substantia nigra (SN), dentate nucleus (DN), thalamus (THA), pulvinar of thalamus (PT). The Mini‐Mental State Examination (MMSE) test and clinical factors were recorded.Statistical TestingAnalysis of covariance adjusting for age and gender as covariates or a paired t‐test for the differences in susceptibility, MMSE scores, and clinical factors among baseline, follow‐up patients, and HCs. Correlation and stepwise regression analysis for the relationship between susceptibility, MMSE scores, and clinical factors.ResultsThe susceptibility of bilateral CN, GP, PUT, RN, SN, DN, THA, PT in follow‐up patients was significantly higher than that in baseline between patients and HCs except for left THA (all P < 0.05; Bonferroni corrected). MMSE scores significantly negatively correlated with the susceptibility of bilateral CN, PUT, and RRN in the baseline examination and bilateral CN, PUT, RN, and DN in the follow‐up examination (all P < 0.05; false discovery rate [FDR] corrected). The follow‐up interval, creatinine, phosphorus, and calcium were independent factors for the increased susceptibility of some nuclei (all P < 0.05).Data ConclusionThe iron deposition of gray matter nuclei in hemodialysis patients increased over roughly a 2‐year period and may be a risk factor for neurocognitive impairment. Creatinine and abnormal calcium‐phosphorus metabolism were independent risk factors for abnormal iron deposition.Level of Evidence: 2Technical Efficacy: Stage 1J. Magn. Reson. Imaging 2019;49:786–799.
Anatomical location, sex, and age influence murine arterial circumferential cyclic strain before and during dobutamine infusion
Authors: Castle Paige E. , Scheven Ulrich M. , Crouch A. Colleen , Cao Amos A. , Goergen Craig J. , Greve Joan M. 
Published: October 2018

BackgroundOne of the primary biomechanical factors influencing arterial health is their deformation across the cardiac cycle, or cyclic strain, which is often associated with arterial stiffness. Deleterious changes in the cardiovascular system, e.g., increased arterial stiffness, can remain undetected until the system is challenged, such as under a cardiac stressor like dobutamine.PurposeTo quantify cyclic strain in mice at different locations along the arterial tree prior to and during dobutamine infusion, while evaluating the effects of sex and age.Study TypeControl/cohort study.Animal ModelTwenty C57BL/6 mice; male, female; ∼12 and 24 weeks of age; n = 5 per group.Field Strength/Sequence7T; CINE MRI with 12 frames, velocity compensation, and prospective cardiac gating.AssessmentPrior to and during the infusion of dobutamine, Green–Lagrange circumferential cyclic strain was calculated from perimeter measurements derived from CINE data acquired at the carotid artery, suprarenal and infrarenal abdominal aorta, and iliac artery.Statistical TestsAnalysis of variance (ANOVA) followed by post‐hoc tests was used to evaluate the influence of dobutamine, anatomical location, sex, and age.ResultsHeart rates did not differ between groups prior to or during dobutamine infusion (P = 0.87 and P = 0.08, respectively). Dobutamine increased cyclic strain in each group. Within a group, increases in strain were similar across arteries. At the suprarenal aorta, strain was reduced in older mice at baseline (young 27.6 > mature 19.3%, P = 0.01) and during dobutamine infusion (young 53.0 > mature 36.2%, P = 0.005). In the infrarenal aorta, the response (dobutamine – baseline) was reduced in older mice (young 21.9 > mature 13.5%, P = 0.04).Data ConclusionDobutamine infusion increases circumferential cyclic strain throughout the arterial tree of mice. This effect is quantifiable using CINE MRI. The results demonstrate that strain prior to and during dobutamine is influenced by anatomical location, sex, and age.Level of Evidence: 3Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;49:69–80.
Anthocyanidins structural study using positive electrospray ionization triple quadrupole mass spectrometry and H/D exchange
Authors: Poliseli Camila B. , Ribeiro Marcos , Tonin Angelica P.P. , Vagula Julianna M. , Santos Oscar O. , Visentainer Jesuí V. , Pontes Rodrigo M. , Moraes Luiz A.B. , Meurer Eduardo C. 
Published: October 2018

AbstractWe report herein a detailed structural study by collision‐induced dissociation (CID) of nonglycosylated anthocyanins (anthocyanidins) using electrospray ionization triple quadrupole mass spectrometry (ESI‐QqQ) and isotope labeling experiments to understand the fragmentation process often used in mass spectrometry analysis of this class of compounds. Tandem mass spectrometric product ion spectra for three anthocyanidins (cyanidin, delphynidin, and pelargonin) were evaluated to propose fragmentation mechanisms to this natural colorant class of organic compounds. The proposed rearrangements, retro Diels‐Alder reaction, water loss, CO losses, and stable acylium ion formation, were evaluated based on tandem mass spectrometric experiments of normal and labeled precursor ions together to computational thermochemistry. B3LYP/6‐311 + G** ab initio calculations studies were carried out to obtain energy diagrams to show the viability of the proposed mechanisms. The CO losses fragmentation channels have lower energies when compared with water losses and the other proposed fragmentations. The isotope labeling experiments indicate the H/D exchange of the hydroxyl protons and corroborate the proposed general fragmentation mechanism for anthocyanidins.
Interfacial Behavior of Anionic/Cationic Flotation Collectors in Mixed Aqueous Solutions and Their Effect on Flotation Recovery of Quartz
Authors: Özün Savaş , Ulutaş Şemsettin 
Published: October 2018

AbstractIn this study, the effects of anionic/cationic collector mixtures on surface tension (γ) were investigated as a function of pH using the pendant drop method. For the tests, long‐chain tallow amine and petroleum sulfonate, which are the most common anionic and cationic flotation collectors, were used individually and as binary mixtures with different mixing ratios. The results show that pH of the solution had crucial effects on surface tension values of the amine whose species distribution varied with pH but not on petroleum sulfonate that is found in the ionized form over the entire pH range. In the case of binary anionic/cationic collector mixtures, the minimum surface tension values were obtained under highly acidic pH conditions with a mixing ratio of 1:3, while the collector mixtures with 3:1 and 1:1 mixing ratios displayed similar surface tension values at all pH values. For all binary‐collector mixtures with different mixing ratios, the surface tension values were found to be higher than those of single‐collector solutions with equal collector concentrations. In the case of microflotation tests, the flotation recoveries of quartz in the presence of the binary anionic/cationic collector mixtures dropped more than 25% when compared with the results in the presence of single‐collector solutions having equal collector concentrations.
Critical evaluation of microextraction pretreatment techniques – Part 1: Single drop and sorbent‐based techniques
Authors: Havlikova Martina , Cabala Radomir , Pacakova Vera , Bursova Miroslava , Bosakova Zuzana 
Published: October 2018

AbstractSample pretreatment techniques or preconcentration constitute a very important step before the analysis of environmental, clinical, pharmaceutical, and other complex samples. Thanks to extraction techniques it is possible to achieve higher method sensitivities and selectivities. Miniaturization microextraction methods make them more environmentally friendly and only small amounts of samples are required. In the past 30 years, a number of microextraction methods have been developed and used and are documented in thousands of articles. Many reviews have been written focusing on their use in specified professional fields or on the latest trends. Unfortunately, no uniform nomenclature has been introduced for these methods. Therefore, this review attempts to classify all the essential microextraction techniques and describes their advantages, disadvantages, and the latest innovations. The methods are divided into two main groups: single drop and sorbent‐based techniques according to the type of extraction phase.