bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cost‐utility analysis of once‐weekly insulin icodec and once‐daily insulin glargine in patients with type 2 diabetes receiving basal‐bolus insulin therapy in China
Authors: Dai Nan , Su Xiaorong , Wang Yong 
Published: October 2024

AbstractObjectiveThe purpose of this study is to explore the rational pricing range for the once‐weekly administration of insulin icodec in the treatment of type 2 diabetes patients in China who have already received basal insulin therapy.MethodsThe data foundation of this study originates from the ONWARDS 4 clinical trial and research materials on Chinese type 2 diabetes patients. By comprehensively applying cost‐utility analysis methods and binary search techniques, the appropriate price positioning of insulin icodec was determined from the perspective of China's healthcare system.ResultsIn the long‐term treatment simulation, we found that insulin icodec and insulin glargine performed similarly in terms of quality‐adjusted life years (QALYs), with 10.15 and 10.07 years, respectively. Although the annual cost of insulin icodec was initially assumed to be equivalent to that of insulin glargine, in‐depth analysis revealed that insulin icodec may have higher cost‐effectiveness potential. Further price sensitivity analysis indicated that the reasonable cost range of insulin icodec lies between $851.95 and $1358.25. After fine‐tuning through univariate sensitivity analysis, this cost range was revised to $784.90 to $1145.96, a conclusion that was robustly validated in subsequent probabilistic sensitivity analysis and scenario simulations.ConclusionThe conclusion drawn from this study is that, with insulin glargine as the cost reference, the economic cost of insulin icodec for Chinese type 2 diabetes patients is expected to range from $784.90 to $1145.96, providing a reference basis for clinical decision‐making and healthcare policy formulation.
The alteration of serotonergic markers in the amygdala and raphe nuclei of oestrogen receptor β knock‐out female mice
Authors: Kalinowski Daniel , Bogus‐Nowakowska Krystyna 
Published: October 2024

AbstractThe amygdala and raphe nuclei, which play crucial roles in mood regulation, are influenced by serotonergic neurotransmission. Alterations in this neurotransmission are associated with mood disorders. Therefore, using immunohistochemistry and quantitative methods this study was designed to evaluate potential alterations in the expression of serotoninergic markers in the amygdala and raphe nuclei of mice with oestrogen receptor β (ERβ) knock out which exhibit increased anxiety as evidenced by reduced locomotion and increased thigmotaxis. These alterations could either contribute to heightened anxiety or serve as a compensatory strategy for reducing it. The results show that in ERβ knock‐out mice, 5‐HT1B expression is significantly increased in the amygdala, while 5‐HTT expression is significantly decreased in both the amygdala and raphe nuclei. Furthermore, ERβ deficiency does not affect TPH2. In conclusion, serotonin signalling is altered in the amygdala and raphe nuclei of ERβ knock‐out females. It seems that an increase in 5‐HTT levels has been associated with reduced anxiety, whereas a decrease in 5‐HT1B receptors may encourage fear. However, further studies are required to determine the exact role of ERβ in anxiety‐related behaviour.
Prevalence and predictors of help‐seeking steps in a nationally representative Dutch sample of romantic couples
Authors: Conradi Henk Jan , Noordhof Arjen , Kamphuis Jan H. 
Published: October 2024

AbstractCouples appear to frequently experience relationship problems, yet estimates of the prevalence and prediction of three distinct help‐seeking steps, (1) recognition of serious relationship dissatisfaction, (2) considering help, and (3) receiving relationship help, are unknown for representative population samples. This is unfortunate as such knowledge may inform policy makers in the development of strategies to motivate couples to seek help. The prevalence of these steps along with reasons for not acquiring help was studied in a representative population sample of 1014 Dutch heterosexual couples. Multilevel Actor‐Partner Interdependence Modeling (APIM) analyses estimated the extent to which static socio‐demographic factors were predictive of help‐seeking behaviors. Of all partners, 28.6% reported having ever been seriously dissatisfied with their relationship (step 1), of which 86.2% had told their partner they were dissatisfied, on average 1.5 years after onset of the dissatisfaction. Of the seriously dissatisfied partners 36.4% considered professional relationship help (step 2) and 19.5% eventually received help (step 3), on average 3.7 years after the onset of dissatisfaction. Of these, 88.5% consulted a (couple) therapist. Main reasons for not acquiring help were that “things got better” (48.8%) and “the partner did not want relationship help” (35.4%), whereas financial considerations and shame were rarely endorsed. Although serious relationship dissatisfaction was common (i.e., 40.1% of all couples consisted of at least one partner who was ever dissatified), few couples sought help and they did so rather late. Waxing and waning of dissatisfaction often guided the decision to not seek help. Women and younger partners were more proactive in help‐seeking. Marital status, having children, and education were largely unrelated to help‐seeking.
Knowledge, practices and perceptions of energy labelling of nut products among Australian consumers and stakeholders: an online survey
Authors: Nikodijevic Cassandra J. , Probst Yasmine C. , Tan Sze‐Yen , Neale Elizabeth P. 
Published: October 2024

AbstractBackgroundNut consumption in Australia is low, perhaps due to concerns with weight gain. However, nut consumption is not associated with increased weight, in part because of their lower metabolisable energy. Current energy labelling for nuts use Atwater factors, which over‐represent the available energy from nuts. Therefore, this research aimed to examine stakeholders' knowledge, practices and perceptions of displaying true metabolisable energy on nutrition labels and its perceived impact on nut consumption.MethodsAn anonymous and voluntary survey was conducted online. Eligible respondents lived in Australia, were aged 18 years or older and had either no formal nutrition education (consumer group) or had formal training and were working as a stakeholder (stakeholder group; working in nutrition/dietetics, public health, food industry, food regulation or nut growing). Convenience sampling and snowballing were used to recruit respondents primarily via online advertisement.ResultsThree hundred and forty‐eight respondents (63.2% female; 71.0% aged 18–39 years; 69.0% Bachelor's degree or higher) were included in the analyses. A larger proportion of stakeholders agreed that nut consumption assists with weight management (67.9%) compared with consumers (47.6%). Theoretical food packaging showing both Atwater and metabolisable energy in the nutrition information panel was favoured among consumers and stakeholders, though 62.3% of consumers reported that a lower metabolisable energy of nuts would not impact their consumption.ConclusionsThis study indicates a need to further investigate the impact of metabolisable energy labelling on nut intake. Further education about the relationship between nuts and body weight is required for consumers and stakeholders.
Bertiella mucronata in Black‐and‐Gold Howler Monkey (Alouatta caraya) in Minas Gerais State, Brazil
Authors: Debortoli Gabriele Zaine Teixeira , Gomes‐Batista Victor Luiz , Ribeiro Cibelli Silva , Ramos Dirceu Guilherme de Souza , Moraes Iago de Sá , Castilho Maíra Sales , Zanfagnini Leticia Gomes , Dias Neto Ramiro das Neves , Sousa Carlos Augusto dos Santos , Santos‐Doni Thaís Rabelo 
Published: October 2024

ABSTRACTThis is the first reported case of Bertiella mucronata infection in a black‐and‐gold howler monkey (Alouatta caraya) in Brazil. The present study confirms the widespread of B. mucronata in South America and suggest that black‐and‐gold howler monkeys may serve as viable reservoirs for B. mucronata.
The Growth Performance, Haematological Parameters and Serum Biochemistry of the Flathead Grey Mullet Mugil cephalus Under Recirculating Aquaculture System and Traditional Culture Systems
Authors: Badran Mohamed F. , Ali Mervat A. M. , Yusuf Mohamed S. 
Published: October 2024

ABSTRACTThe present investigation explored the growth performance, haematological parameters and serum biochemistry of flathead grey mullet under recirculating aquaculture system (RAS) and traditional culture systems. Nine hundred healthy fish, weighing 43.80 ± 3.55 g and length 16.54 ± 0.82 cm were randomly distributed to three treatments of RAS and traditional culture systems (T1: 20% of water changed daily; T2: once‐through system 250 L/h; T3: RAS—20% of water changed weekly) in nine concrete tanks of 4000 L at stocking density of 100 fish/tank and each treatment had three replicates. Fish were fed (33.1% crude protein, 3 mm pellets) twice a day at a rate of 3% for 12 weeks. These findings suggest that the T3 followed by T2 led to notable improvements in growth performance compared to other culture systems. Significantly elevated values of weight gain, length gain, specific growth rate, average daily gain and survival % were observed in T3. The RBCs exhibited a significant decrease under T2. Haemoglobin concentrations and WBC counts significantly increased under RAS. Significant values of mean cell haemoglobin and mean cell haemoglobin concentration were recorded in T2. Total protein, albumin and globulin enhanced significantly under RAS, whereas the highest values of cortisol were observed in T2 and T3. These findings demonstrate that the RAS followed by once‐through system significantly improve growth performance, feed efficiency and survival, as well as enhancing the haematology and serum biochemical status of flathead grey mullet. In conclusion, RAS provides ideal conditions for optimizing physiological functions, growth performance and fish health.
Restricted Dispersal and Phenotypic Response to Water Depth in a Foundation Seagrass
Authors: Sotka Erik E. , Hughes A. Randall , Hanley Torrance C. , Hays Cynthia G. 
Published: October 2024

ABSTRACTSpecies conservation and management benefit from precise understanding of natural patterns of dispersal and genetic variation. Using recent advances in indirect genetic methods applied to both adult plants and dispersed seeds, we find that the mean seed dispersal in a threatened marine foundation plant (the eelgrass Zostera marina) is approximately 100–200 m. This distance is surprisingly more similar to that of wind‐dispersed terrestrial seeds (~10s to 100s of meters) than the passive dispersal of marine propagules via currents (~10s to 100s of kilometres). Because nearshore marine plants like Zostera are commonly distributed across strong selective gradients driven by bathymetry (depth) even within these restricted spatial scales, seeds are capable of dispersing to novel water depths and experiencing profound shifts in light availability, temperature and wave exposure. We documented strong phenotypic variation and genome‐wide differentiation among plants separated by approximately the spatial scale of mean realised dispersal. This result suggests genetic isolation by environment in response to depth‐related environmental gradients as one plausible explanation for this pattern. The ratio of effective to census size (or Ne/Nc) approximated 0.1%, indicating that a fraction of existing plants provides the genetic variation to allow adaptation to environmental change. Our results suggest that successful conservation of seagrass meadows that can adapt to microspatial and temporal variation in environmental conditions will be low without direct and persistent intervention using large numbers of individuals or a targeted selection of genotypes.
Orthopteran Neo‐Sex Chromosomes Reveal Dynamics of Recombination Suppression and Evolution of Supergenes
Authors: Jayaprasad Suvratha , Peona Valentina , Ellerstrand Simon J. , Rossini Roberto , Bunikis Ignas , Pettersson Olga V. , Olsen Remi‐André , Rubin Carl‐Johan , Einarsdottir Elisabet , Bonath Franziska , Bradford Tessa M. , Cooper Steven J. B. , Hansson Bengt , Suh Alexander , Kawakami Takeshi , Schielzeth Holger , Palacios‐Gimenez Octavio M. 
Published: October 2024

ABSTRACTThe early evolution of sex chromosomes has remained obscure for more than a century. The Vandiemenella viatica species group of morabine grasshoppers is highly suited for studying the early stages of sex chromosome divergence and degeneration of the Y chromosome. This stems from the fact that neo‐XY sex chromosomes have independently evolved multiple times by X‐autosome fusions with different autosomes. Here, we generated new chromosome‐level assemblies for two chromosomal races representing karyotypes with and without neo‐sex chromosomes (P24XY and P24X0), and sequence data of a third chromosomal race with a different neo‐XY chromosome system (P25XY). Interestingly, these two neo‐XY chromosomal races are formed by different X‐autosome fusions (involving chr1 and chrB, respectively), and we found that both neo‐Y chromosomes have partly ceased to recombine with their neo‐X counterpart. We show that the neo‐XY chromosomes have diverged through accumulation of SNPs and structural mutations, and that many neo‐Y‐linked genes have degenerated since recombination ceased. However, the non‐recombining regions of neo‐Y chromosomes host non‐degenerated genes crucial for sex determination, such as sex‐lethal and transformer, alongside genes associated with spermatogenesis, fertility, and reproduction, illustrating their integrative role as a masculinizing supergene. Contrary to expectations, the neo‐Y chromosomes showed (slightly) lower density of transposable elements (TEs) compared to other genomic regions. The study reveals the unique dynamics of young sex chromosomes, with evolution of recombination suppression and pronounced decay of (some) neo‐sex chromosome genes, and provides a compelling case illustrating how chromosomal fusions and post‐fusion mutational processes contribute to the evolution of supergenes.
How does religion influence an emerging nationalism? Evidence from the Kurdish context in Turkey
Authors: Caglayan Muttalip 
Published: October 2024

AbstractBased on qualitative interviews with 66 Sunni Muslim Kurdish elites, this study reveals that Kurdish Islamic circles in Turkey are not monolithic, homogeneous or fixed. Some willingly or unwillingly maintain their Islamic identity as a primary reference point for self‐consciousness, motivation for collective action and political aspirations, often at the expense of ethnopolitical mobilization. Others combine Kurdish national claims with an Islamic orientation, pursuing a path of coexistence between the secular and the religious in the expedition towards nationhood. In the competitive interplay between religion and nationalism, Islam acts as a restraining force on the rise of national sentiment among Muslim Kurds. In the symbiotic interplay, Islam no longer serves as an obstacle to delay Kurdish mobilization but rather accommodates secular norms and values in favour of welcoming national zeals.
Effect of BMI on Caries Assessed by Structural Equation Modelling in an Adult Brazilian Population
Authors: dos Santos Moura Maurício , Rup Ariel Goulart , Jardim Juliana Jobim , Haas Alex Nogueira , Rios Fernando Silva , Costa Ricardo dos Santos Araújo , Alves Luana Severo , Celeste Roger Keller , Maltz Marisa 
Published: October 2024

ABSTRACTObjectivesCompare different behavioural, environmental and socioeconomic factors for caries with transversal data to decompose the direct and indirect effects of body mass index (BMI) in relation to coronal and root caries.MethodsThis cross‐sectional study used a representative sample of 1002 individuals aged ≥ 35 years living in Porto Alegre. Questionnaires recorded age, sex, educational level, tooth brushing frequency and access to dental services. Oral examination assessed gingival bleeding and recession, coronal and root caries. Height and weight were collected to calculate the BMI. The structural equation modelling approach was used, and standardised coefficients (SC) to direct, indirect and total effects were calculated.ResultsThe prevalence of excessive body weight was 71.17%, of which 40.09% were overweight and 31.07% were obese. The overall prevalence of coronal and root caries was 99.83 and 36.95, respectively. No direct link between BMI and coronal or root caries was observed. For coronal caries, positive SC was found for age (0.56; p < 0.01) and sex (female) (0.14; p < 0.01). For root caries, positive SC was detected for age (0.34; p < 0.01), smoking exposure (0.17; p < 0.01) and gingivitis (0.08; p < 0.01).ConclusionsOverweight and obese adults should not be regarded at higher risk for dental caries.
“All the horrible emotions have passed, I still remained, and I was safe”: A qualitative study of Lesbian and Gay people's lived experience of completing a full Dialectical Behaviour Therapy programme
Authors: Harding Charlotte , Pratt Daniel , Lea James 
Published: October 2024

AbstractObjectivesLesbian and Gay people (LGP) experience higher rates of mental health difficulties, including self‐harm, suicidal behaviours, as well as inequalities in health care, than their heterosexual peers. Dialectical Behaviour Therapy (DBT) is an evidence‐based treatment for self‐harm and suicidal behaviours, though there is little research on LGP's experiences of DBT. This research aims to explore LGP's experiences of completing a full DBT programme.DesignA qualitative design with semi‐structured individual interviews was utilised. The results were analysed using Interpretative Phenomenological Analysis.MethodsSix lesbian and two gay adults, aged between 22 and 47 years, living in the United Kingdom took part. All participants had completed a full programme of DBT within the 2 years prior to the interview.ResultsFour superordinate themes emerged from the data: (1) The DBT journey; (2) Connections and Sense of Community during DBT; (3) Sexuality both visible and invisible in DBT and (4) A Gender, Sexuality and Relationship Diverse (GSRD) affirmative future for DBT.ConclusionsSeveral clinical implications are suggested to improve DBT for LGP, for example to consider having other LGP within DBT groups, to create a more GSRD‐affirming DBT (changes to the DBT manual, DBT therapists, DBT programme and physical DBT space), to adapt DBT techniques to manage sexuality‐related difficulties and to adopt a GSRD‐centric framework. Overall, DBT appears to be beneficial for LGP.
Modulation of Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus by Sphingomonas Sp Y503 via the CrMAPKKK1‐CrMAPKK1/CrMAPKK2‐CrMPK3 Signaling Cascade
Authors: Gao Xiaoxiao , Zhu Xiaona , Wang Zhiqin , Liu Xuejing , Guo Rui , Luan Jing , Liu Zhiwen , Yu Fang 
Published: October 2024

ABSTRACTCatharanthus roseus is a highly relevant model for investigating plant defense mechanisms and the biosynthesis of therapeutically valuable compounds, including terpenoid indole alkaloids (TIAs). It has been demonstrated that beneficial microbial interactions can regulate TIA biosynthesis in C. roseus, highlighting the need to fully comprehend the molecular mechanisms involved to efficiently implement eco‐friendly strategies. This study explores the effects of a novel microbial strain, Y503, identified as Sphingomonas sp., on TIA production and the underlying mechanisms in C. roseus. Through bioinformatics analysis, we have identified 17 MAPKKKs, 7 MAPKKs, and 13 MAPKs within the C. roseus genome. Further investigation has verified the presence of the MAPK module (CrMAPKKK1‐CrMAPKK1/CrMAPKK2‐CrMPK3) mediating Y503 in regulating TIA biosynthesis in C. roseus. This study provides foundational information for strengthening the plant defense system in C. roseus through advantageous microbial interactions, which could contribute to the sustainable cultivation of medicinal plants such as C. roseus.
UVA Irradiation Promotes Melanoma Cell Proliferation Mediated by OPN3 Independently of ROS Production
Authors: Zhang Yulei , Zeng Wen , Zhang Wei , Wang Yu , Jin Shuqi , Liu Ting , Luo Huanhuan , Lu Hongguang 
Published: October 2024

ABSTRACTUVA radiation, a primary risk factor in melanoma progression, partly acts through the mediation of reactive oxygen species (ROS). The role of ROS in driving cutaneous melanoma toward an invasive phenotype and whether it occurs through opsins (OPNs), which are photosensitive G protein‐coupled receptors, is not fully understood. This study focuses on the impact of UVA radiation on melanoma cell proliferation, with a special emphasis on OPN3. Investigating the biphasic response to various UVA doses (0.75–9 J/cm2) in A375 and MV3 cell lines, and using EdU and CCK‐8 assays, we observed dose‐dependent changes in cell proliferation. Interestingly, UVA irradiation at these doses of 0.75, 1.5 and 3 J/cm2 did not significantly induce ROS production. Our study further delves into the role of OPN3, a photosensitive receptor, in melanoma progression. Following UVA exposure, an increase in OPN3 expression was observed in melanoma cells lines A375 and MV3, indicating its role as a UVA‐sensitive sensor and its influence on cell proliferation. Additionally, UVA‐induced calcium flux in two melanoma cells lines pointed to a calcium‐dependent G protein‐coupled pathway in melanoma proliferation, mediated by OPN3 and not reliant on ROS. This research sheds light on the mechanism of UVA‐induced melanoma progression, underscoring OPN3 as a pivotal regulator and advancing our understanding of UVA's interaction with opsins in melanoma progression.
Metabolite analysis of peach (Prunus persica L. Batsch) branches in response to freezing stress
Authors: Li Y. , Wang Y. , Wang Z. , Liu G. , Chang R. , Chen H. , Li J. , Tian Q. 
Published: October 2024

ABSTRACT
Cold resistance in fruit trees has a direct impact on food production and scientific studies. ‘Donghe No.1’ is an excellent cold‐tolerant peach variety. Metabolomic changes under freezing stress were examined to understand the mechanisms of cold adaptation.
The UPLC–MS/MS system was used to identify differentially expressed metabolites (DEMs) in branches of ‘Donghe No.1’ under freezing stress for 12 h at −5°C, −20°C, −25°C, or −30°C.
In total, 1096 metabolites and 196 DEMs were obtained at −5°C vs −20°C, −25°C, and − 30°C, while 179 DEMs and eight shared DEMs obtained at −5°C vs −20°C, −20°C vs −25°C, and −25°C vs −30°C. KEGG enrichment identified 196 DEMs associated with amino acid metabolism, linoleic acid metabolism, alpha‐linolenic acid metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis under freezing stress.
A metabolic network in 1‐year‐old peach branches under freezing stress is proposed. Moreover, these results enhance understanding of metabolite responses and mechanisms to freezing stress in peach and will help in future breeding of freezing‐tolerant varieties and investigating tolerance mechanisms.