bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reconfiguring surface functions using visible-light-controlled metal-ligand coordination
Authors: Xie Chaoming , Sun Wen , Lu Hao , Kretzschmann Annika , Liu Jiahui , Wagner Manfred , Butt Hans-Jürgen , Deng Xu , Wu Si 
Published: September 2018

AbstractMost surfaces are either static or switchable only between “on” and “off” states for a specific application. It is a challenge to develop reconfigurable surfaces that can adapt to rapidly changing environments or applications. Here, we demonstrate fabrication of surfaces that can be reconfigured for user-defined functions using visible-light-controlled Ru–thioether coordination chemistry. We modify substrates with Ru complex Ru-H2O. To endow a Ru-H2O-modified substrate with a certain function, a functional thioether ligand is immobilized on the substrate via Ru–thioether coordination. To change the surface function, the immobilized thioether ligand is cleaved from the substrate by visible-light-induced ligand dissociation, and then another thioether ligand with a distinct function is immobilized on the substrate. Different thioethers endow the surface with different functions. Based on this strategy, we rewrite surface patterns, manipulate protein adsorption, and control surface wettability. This strategy enables the fabrication of reconfigurable surfaces with customizable functions on demand.
Positive cardiac inotrope omecamtiv mecarbil activates muscle despite suppressing the myosin working stroke
Authors: Woody Michael S. , Greenberg Michael J. , Barua Bipasha , Winkelmann Donald A. , Goldman Yale E. , Ostap E. Michael 
Published: September 2018

AbstractOmecamtiv mecarbil (OM) is a positive cardiac inotrope in phase-3 clinical trials for treatment of heart failure. Although initially described as a direct myosin activator, subsequent studies are at odds with this description and do not explain OM-mediated increases in cardiac performance. Here we show, via single-molecule, biophysical experiments on cardiac myosin, that OM suppresses myosin’s working stroke and prolongs actomyosin attachment 5-fold, which explains inhibitory actions of the drug observed in vitro. OM also causes the actin-detachment rate to become independent of both applied load and ATP concentration. Surprisingly, increased myocardial force output in the presence of OM can be explained by cooperative thin-filament activation by OM-inhibited myosin molecules. Selective suppression of myosin is an unanticipated route to muscle activation that may guide future development of therapeutic drugs.
Controlling factor of incoming plate hydration at the north-western Pacific margin
Authors: Fujie Gou , Kodaira Shuichi , Kaiho Yuka , Yamamoto Yojiro , Takahashi Tsutomu , Miura Seiichi , Yamada Tomoaki 
Published: September 2018

AbstractHydration of the subducting oceanic plate determines the amount of water transported from Earth’s surface into its interior, and plate bending-related faulting (bend faulting) just prior to subduction is considered to promote hydration. Bend faulting shows significant spatial variation, but its contribution to hydration is still poorly understood. Here we present the results of controlled-source seismic surveys around the junction of the Japan and Kuril trenches. We found structural changes caused by bend faulting before subduction differed distinctly between both trenches and were well correlated with plate hydration after subduction, suggesting the bend faulting controls spatial variations in plate hydration. Differences in bend faulting are closely related to the angle between the current trench and the ancient spreading ridge, and the hydration is more extensive where this trench-ridge angle is oblique in the study area. Thus, we propose this angle is a major factor controlling plate hydration.
Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo
Authors: Kotini Maria , Barriga Elias H. , Leslie Jonathan , Gentzel Marc , Rauschenberger Verena , Schambony Alexandra , Mayor Roberto 
Published: September 2018

AbstractConnexins are the primary components of gap junctions, providing direct links between cells under many physiological processes. Here, we demonstrate that in addition to this canonical role, Connexins act as transcriptional regulators. We show that Connexin 43 (Cx43) controls neural crest cell migration in vivo by directly regulating N-cadherin transcription. This activity requires interaction between Cx43 carboxy tail and the basic transcription factor-3, which drives the translocation of Cx43 tail to the nucleus. Once in the nucleus they form a complex with PolII which directly binds to the N-cadherin promoter. We found that this mechanism is conserved between amphibian and mammalian cells. Given the strong evolutionary conservation of connexins across vertebrates, this may reflect a common mechanism of gene regulation by a protein whose function was previously ascribed only to gap junctional communication.
Androgen receptor expression in normal breast tissue and subsequent breast cancer risk
Authors: Kensler Kevin H. , Beca Francisco , Baker Gabrielle M. , Heng Yujing J. , Beck Andrew H. , Schnitt Stuart J. , Hazra Aditi , Rosner Bernard A. , Eliassen A. Heather , Hankinson Susan E. , Brown Myles , Tamimi Rulla M. 
Published: September 2018

AbstractSex steroid hormone signaling is critical in the development of breast cancers, although the role of the androgen receptor remains unclear. This study evaluated androgen receptor (AR) expression in normal breast tissue as a potential marker of breast cancer risk. We conducted a nested case–control study of women with benign breast disease (BBD) within the Nurses’ Health Studies. Epithelial AR expression was assessed by immunohistochemistry in normal tissue from the BBD biopsy and the percent of positive nuclei was estimated in ordinal categories of 10% for 78 breast cancer cases and 276 controls. Logistic regression models adjusting for the matching factors and BBD lesion type were used to calculate odds ratios (ORs) for the association between AR expression (tertiles: ≤10%, 11–30%, and >30%) and breast cancer risk. AR expression in normal breast tissue was not associated with subsequent breast cancer risk (ORT3vsT1 = 0.9, 95% CI = 0.4–1.8, p trend = 0.68). In comparison with low AR/low ER women, ORs of 0.4 (95% CI = 0.1–1.2) for high AR/high ER women, 1.8 (95% CI = 0.4–7.8) for low AR/high ER women, and 0.7 (95% CI = 0.3–1.6) for high AR/low ER women were observed (p interaction = 0.21). Ki67 did not modify the association between AR expression and breast cancer risk (p interaction = 0.75). There was little evidence for an overall association between AR expression in normal breast tissue and breast cancer risk. These findings did not show that the AR association varied by Ki67 expression in normal breast tissue, though there was suggestive heterogeneity by ER expression.
Dvl3 polymorphism interacts with life events and pro-inflammatory cytokines to influence major depressive disorder susceptibility
Authors: Zhang Jian , Yang Jiarun , Han Dong , Zhao Xueyan , Ma Jingsong , Ban Bo , Zhu Xiongzhao , Yang Yanjie , Cao Depin , Qiu Xiaohui 
Published: September 2018

AbstractThe purpose of this study is to explore Dvl3 variants and their interaction with negative life events on MDD susceptibility in a Chinese Han population. Additionally, we also attempted to identify whether there is an association between Dvl3 variants and pro-inflammatory cytokines. A total of 1102 participants, consisting of 550 patients with MDD and 552 healthy subjects, were recruited for genotyping by TaqMan allelic discrimination assay. Pro-inflammatory cytokine mRNA levels in peripheral blood were measured by QPCR. After the assessment of negative life events by the Life Events Scale, the Dvl3 gene–environment interaction (G × E) and risk factors were evaluated using generalized multifactor dimensionality reduction method (GMDR) and logistic regression analysis, respectively. This study is the first to reveal the interaction between Dvl3 allelic variations and negative life events as well as pro-inflammatory cytokines on MDD susceptibility in a Chinese Han population.
Superstatistical distribution of daily precipitation extremes: A worldwide assessment
Authors: De Michele Carlo , Avanzi Francesco 
Published: September 2018

AbstractMaximum annual daily precipitation is a fundamental hydrologic variable that does not attain asymptotic conditions. Thus the classical extreme value theory (i.e., the Fisher-Tippett’s theorem) does not apply and the recurrent use of the Generalized Extreme Value distribution (GEV) to estimate precipitation quantiles for structural-design purposes could be inappropriate. In order to address this issue, we first determine the exact distribution of maximum annual daily precipitation starting from a Markov chain and in a closed analytical form under the hypothesis of stochastic independence. As a second step, we formulate a superstatistics conjecture of daily precipitation, meaning that we assume that the parameters of this exact distribution vary from a year to another according to probability distributions, which is supported by empirical evidence. We test this conjecture using the world GHCN database to perform a worldwide assessment of this superstatistical distribution of daily precipitation extremes. The performances of the superstatistical distribution and the GEV are tested against data using the Kolmogorov-Smirnov statistic. By considering the issue of model’s extrapolation, that is, the evaluation of the estimated model against data not used in calibration, we show that the superstatistical distribution provides more robust estimations than the GEV, which tends to underestimate (7–13%) the quantile associated to the largest cumulative frequency. The superstatistical distribution, on the other hand, tends to overestimate (10–14%) this quantile, which is a safer option for hydraulic design. The parameters of the proposed superstatistical distribution are made available for all 20,561 worldwide sites considered in this work.
High-Resolution AMS Dating of Architecture, Boulder Artworks and the Transition to Farming at Lepenski Vir
Authors: Borić Dušan , Higham Thomas , Cristiani Emanuela , Dimitrijević Vesna , Nehlich Olaf , Griffiths Seren , Alexander Craig , Mihailović Bojana , Filipović Dragana , Allué Ethel , Buckley Michael 
Published: September 2018

AbstractThe archaeological site of Lepenski Vir is widely known after its remarkable stone art sculptures that represent a unique and unprecedented case of Holocene hunter-gatherer creativity. These artworks were found largely associated with equally unique trapezoidal limestone building floors around their centrally located rectangular stone-lined hearths. A debate has raged since the discovery of the site about the chronological place of various discovered features. While over years different views from that of the excavator about the stratigraphy and chronology of the site have been put forward, some major disagreements about the chronological position of the features that make this site a key point of reference in European Prehistory persist. Despite challenges of re-analyzing the site’s stratigraphy from the original excavation records, taphonomic problems, and issues of reservoir offsets when providing radiocarbon measurements on human and dog bones, our targeted AMS (Accelerator Mass Spectrometry) dating of various contexts from this site with the application of Bayesian statistical modelling allows us to propose with confidence a new and sound chronological framework and provide formal estimates for several key developments represented in the archaeological record of Lepenski Vir that help us in understanding the transition of last foragers to first farmers in southeast Europe as a whole.
Comparative Analysis of TM and Cytoplasmic β-barrel Conformations Using Joint Descriptor
Authors: Thangappan Jayaraman , Wu Sangwook , Lee Sun-Gu 
Published: September 2018

AbstractMacroscopic descriptors have become valuable as coarse-grained features of complex proteins and are complementary to microscopic descriptors. Proteins macroscopic geometric features provide effective clues in the quantification of distant similarity and close dissimilarity searches for structural comparisons. In this study, we performed a systematic comparison of β-barrels, one of the important classes of protein folds in various transmembrane (TM) proteins against cytoplasmic barrels to estimate the conformational features using a joint-based descriptor. The approach uses joint coordinates and dihedral angles (β and γ) based on the β-strand joints and loops to determine the arrangements and propensities at the local and global levels. We then confirmed that there is a clear preference in the overall β and γ distribution, arrangements of β-strands and loops, signature patterns, and the number of strand effects between TM and cytoplasmic β-barrel geometries. As a robust and simple approach, we determine that the joint-based descriptor could provide a reliable static structural comparison aimed at macroscopic level between complex protein conformations.
A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins
Authors: Li Xiufeng , Fan Beiyuan , Liu Lixing , Chen Deyong , Cao Shanshan , Men Dong , Wang Junbo , Chen Jian 
Published: September 2018

AbstractThis study presents a microfluidics based cytometry capable of characterizing cell sizes and counting numbers of specific cytosolic proteins where cells were first bound by antibodies labelled with fluorescence and then aspirated into a constriction microchannel in which fluorescent levels were measured. These raw fluorescent pulses were further divided into a rising domain, a stable domain and a declining domain. In addition, antibody solutions with labelled fluorescence were aspirated through the constriction microchannel, yielding curves to translate raw fluorescent levels to protein concentrations. By using key parameters of three domains as well as the calibration curves, cell diameters and the absolute number of β-actins at the single-cell level were quantified as 14.2 ± 1.7 μm and 9.62 ± 4.29 × 105 (A549, ncell = 14 242), 13.0 ± 2.0 μm and 6.46 ± 3.34 × 105 (Hep G2, ncell = 35 932), 13.8 ± 1.9 μm and 1.58 ± 0.90 × 106 (MCF 10 A, ncell = 16 650), and 12.7 ± 1.5 μm and 1.09 ± 0.49 × 106 (HeLa, ncell = 26 246). This platform could be further adopted to measure numbers of various cytosolic proteins, providing key insights in proteomics at the single-cell level.
Acoustic properties of vowel production in Mandarin-speaking patients with post-stroke dysarthria
Authors: Mou Zhiwei , Chen Zhuoming , Yang Jing , Xu Li 
Published: September 2018

AbstractThis study investigated the acoustic features of vowel production in Mandarin-speaking patients with post-stroke dysarthria (PSD). The subjects included 31 native Mandarin-speaking patients with PSD (age: 25–83 years old) and 38 neurologically normal adults in a similar age range (age: 21–76 years old). Each subject was recorded producing a list of Mandarin monosyllables that included six monophthong vowels (i.e., /a, i, u, ɤ, y, o/) embedded in the /CV/ context. The patients’ speech samples were evaluated by two native Mandarin speakers. The evaluation scores were then used to classify all patients into two levels of severity: mild or moderate-to-severe. Formants (F1 and F2) were extracted from each vowel token. Results showed that all vowel categories in the patients with PSD were produced with more variability than in the healthy speakers. Great overlaps between vowel categories and reduced vowel space were observed in the patients. The magnitude of the vowel dispersion and overlap between vowel categories increased as a function of the severity of the disorder. The deviations of the vowel acoustic features in the patients in comparison to the healthy speakers may provide guidance for clinical rehabilitation to improve the speech intelligibility of patients with PSD.
Stationary Computed Tomography for Space and other Resource-constrained Environments
Authors: Cramer Avilash , Hecla Jake , Wu Dufan , Lai Xiaochun , Boers Tim , Yang Kai , Moulton Tim , Kenyon Steven , Arzoumanian Zaven , Krull Wolfgang , Gendreau Keith , Gupta Rajiv 
Published: September 2018

AbstractComputed tomography (CT) is used to diagnose many emergent medical conditions, including stroke and traumatic brain injuries. Unfortunately, the size, weight, and expense of CT systems make them largely inaccessible for patients outside of major hospitals. We have designed a module containing multiple miniature x-ray sources that could allow for CT systems to be significantly lighter, smaller, and cheaper, and to operate without any moving parts. We have developed a novel photocathode-based x-ray source, created by depositing a thin film of magnesium on an electron multiplier. When illuminated by a UV LED, this photocathode emits a beam of electrons, with a beam current of up to 1 mA. The produced electrons are accelerated through a high voltage to a tungsten target. These sources are individually addressable and can be pulsed rapidly, through electronic control of the LEDs. Seven of these sources are housed together in a 17.5 degree arc within a custom vacuum manifold. A full ring of these modules could be used for CT imaging. By pulsing the sources in series, we are able to demonstrate x-ray tomosynthesis without any moving parts. With a clinical flat-panel detector, we demonstrate 3D acquisition and reconstructions of a cadaver swine lung.
Site-directed mutagenesis of Campylobacter concisus respiratory genes provides insight into the pathogen’s growth requirements
Authors: Benoit Stéphane L. , Maier Robert J. 
Published: September 2018

AbstractCampylobacter concisus is an emerging human pathogen found throughout the entire human oral-gastrointestinal tract. The ability of C. concisus to colonize diverse niches of the human body indicates the pathogen is metabolically versatile. C. concisus is able to grow under both anaerobic conditions and microaerophilic conditions. Hydrogen (H2) has been shown to enhance growth and may even be required. Analysis of several C. concisus genome sequences reveals the presence of two sets of genes encoding for distinct hydrogenases: a H2-uptake-type (“Hyd”) complex and a H2-evolving hydrogenase (“Hyf”). Whole cells hydrogenase assays indicate that the former (H2-uptake) activity is predominant in C. concisus, with activity among the highest we have found for pathogenic bacteria. Attempts to generate site-directed chromosomal mutants were partially successful, as we could disrupt hyfB, but not hydB, suggesting that H2-uptake, but not H2-evolving activity, is an essential respiratory pathway in C. concisus. Furthermore, the tetrathionate reductase ttrA gene was inactivated in various C. concisus genomospecies. Addition of tetrathionate to the medium resulted in a ten-fold increase in cell yield for the WT, while it had no effect on the ttrA mutant growth. To our knowledge, this is the first report of mutants in C. concisus.
Dose-dependent behavioural fever responses in desert locusts challenged with the entomopathogenic fungus Metarhizium acridum
Authors: Clancy Lisa M. , Jones Rory , Cooper Amy L. , Griffith Gareth W. , Santer Roger D. 
Published: September 2018

AbstractBehavioural fever is a common response to immune challenge in ectotherms and confers survival benefits. However, costs accrue rapidly as body temperature rises. Thus, the magnitude of adaptive fever responses might reflect the balance of costs and benefits. We investigated behavioural fever in desert locusts, Schistocerca gregaria, infected with the entomopathogenic fungus Metarhizium acridum. We first tracked the time course of behavioural fever in infected locusts, demonstrating that body temperatures rose on the day following inoculation (day 1), and reached peak intensity on the day after that (day 2). Subsequently, the magnitude of fever responses varied during a day, and locusts tended to exhibit high-intensity fever responses in the mornings when basking was first possible. We speculate that this may have resulted from increased fungal load caused by unimpeded growth overnight when locusts could not fever. We next inoculated locusts with different M. acridum doses ranging from 0 to ca. 75,000 conidia. The magnitude of their behavioural fever responses on day 2 post-inoculation was positively related to fungal dose. Thus, we demonstrate dose-dependency in the behavioural fever responses of desert locusts and suggest that this may reflect the adaptive deployment of behavioural fever to minimize costs relative to benefits.
In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption
Authors: Anderson Annette Carola , Rothballer Michael , Altenburger Markus Jörg , Woelber Johan Peter , Karygianni Lamprini , Lagkouvardos Ilias , Hellwig Elmar , Al-Ahmad Ali 
Published: September 2018

AbstractCaries is associated with shifts of microbiota in dental biofilms and primarily driven by frequent sucrose consumption. Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm. Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples. After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase. The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region). In addition, roughness of the enamel surface was measured with laser scanning microscopy. The sucrose phase resulted in significant differences in beta-diversity and significantly decreased species richness. It was marked by a significant increase in abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis. Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface. The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.