bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Docking Analysis of Herbal Medicines for Diabetes Mellitus Treatment
Authors: Sethi Amna , Haleem Madiha , Shar Nisar Ahmed 
Published: December 2024

Background:
Diabetes Mellitus is a global health concern affecting millions of individuals
worldwide. It is characterized by hyperglycemia resulting from insufficient insulin production
or insulin resistance. Its high prevalence, especially in developing countries, such as Pakistan,
demands effective and economically viable treatments.

Objectives:
The objective of this study was to identify herbal remedies for effectively targeting
gluconeogenic enzymes in the treatment of diabetes mellitus. It aimed to explore herbal remedies
for providing cost-effective alternatives with lesser side effects for the treatment of diabetes by
targeting gluconeogenic enzymes.

Methods:
The proteins of the already-known diabetic herb Momordica Charantia (Bitter melon)
were initially investigated through molecular docking. Then, the proteins similar to the bestdocked
protein of Momordica Charactia were identified, and their molecular docking analysis
was performed.

Results:
The results showed three plants having similar proteins to the Momrodica Charantia
best-docked protein, namely Luffa Aegyptiaca (sponge gourd), Cucumis Sativus (cucumber),
and Cucurbita Maxima (Pumpkin). The molecular docking analysis of these proteins revealed
Cucumis Sativus (Cucumber) as a potential herbal candidate for the treatment of diabetes
mellitus.

Conclusion:
The results of this study highlight the potential of Cucumis Sativus (Cucumber) for
treating diabetes mellitus by targeting gluconeogenic enzymes. However, further research is necessary
regarding the therapeutic potential of cucumber.
Viability of Extracorporeal Membrane Oxygenation Circuits during Arterio-venous Shunting: An Observational Study of ECMO Circuits
Authors: Shrimpton Nicole YR , Black Anthony P , Venugopal Prem S , Alphonso Nelson , Mattke Adrian C 
Published: December 2024

Introduction
Trial off veno-arterial extracorporeal membrane oxygenation is often performed by diversion of circuit flow through an arterio-venous shunt. Separation from the patient presents an augmented risk for microbiological growth and deterioration of the circuit components and blood circulating within it. It is unclear how long mechanical components and blood circulating within the circuit remain intact during arterio-venous shunting.

Methods
Following decannulation, the arterial and venous lines were connected simulating an arterio-venous shunt. Flow was maintained at 500 ml/min. Observations at 11 intervals were paired with blood culture, blood gases, chemistry and coagulation studies. Heparin was not provided in two circuits, and four circuits received 30 IU/hour.

Results
Of six circuits studied, five maintained flows of 500 ml/min for 48 hours. One circuit developed extensive clot after two hours and blood flow ceased. Microbial growth was not detected. Glucose, fibrinogen and platelet levels reduced progressively from the outset. Cell breakdown was evident by four hours (increase in plasma potassium and phosphate) and lactate rose above 6.3 mmol/L at four hours in all circuits.

Conclusion
Arterio-venous shunting longer than four hours carries a risk of blood decomposition and cell energy pathway depletion. Re-establishing extracorporeal membrane oxygenation support post arterio-venous shunting carries a risk of physiological derangement, given the blood composition changes during arterio-venous shunting. Consideration should be given to an alternate method to assess the physiological stability of patients off extracorporeal membrane oxygenation prior to decannulation. Alternately, consider a crystalloid flush of the circuit to remove decomposed blood if the trial-off exceeds four hours.
Pengaruh Penambahan Infusa Daun Pandan Wangi	(Pandanus amaryllifolius) dalam Air Minum terhadap Organ Hati, Jantung, Limpa dan Usus
Authors: Sihombing Sarah Nadia , Malvin Toni , Fati Nelzi , Nilawati Nilawati 
Published: December 2024

Latar Belakang: Daun pandan wangi (Pandanus amaryllifolius) memiliki kandungan senyawa bioaktif seperti alkaloid, flavonoid, dan tanin yang memiliki sifat antioksidan, antimikroba, dan anti-inflamasi. Tujuan: Tujuan penelitian adalah untuk mengetahui pengaruh penambahan infusa daun pandan wangi dalam air minum terhadap persentase hati, jantung, limpa, panjang usus dan berat usus broiler. Metode: Penelitian ini menggunakan 100 ekor broiler strain cobb umur satu hari tanpa pemisahan jenis kelamin yang dibagi secara acak menjadi 20 unit percobaan. Masing- masing unit percobaan terdiri dari 5 ekor. Ayam dipelihara selama 4 minggu, dan pengumpulan data dilakukan pada minggu ke 4 saat panen. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan 4 perlakuan infusa daun pandan wangi dalam air minum dan dengan 5 ulangan. Masing-masing perlakuan yaitu: P0: tanpa penambahan infusa daun pandan wangi, P1: penambahan infusa daun pandan wangi 1% dalam air minum, P2: penambahan infusa daun pandan wangi 2% dalam air minum, dan P3: penambahan infusa daun pandan wangi 3% dalam air minum. Hasil: Hasil penelitian menunjukkan bahwa perlakuan penambahan infusa daun pandan wangi dalam air minum berpengaruh nyata terhadap bobot limpa (P<0,05). Hasil penelitian menunjukkan bahwa perlakuan penambahan infusa daun pandan wangi dalam air minum tidak berpengaruh nyata (P>0,05) terhadap bobot hati, jantung, panjang usus dan bobot usus broiler. Kesimpulan: Berdasarkan hasil penelitian dan pembahasan dapat disimpulkan bahwa respon fisiologis broiler pemberian infusa daun pandan wangi dalam air minum berpengaruh nyata terhadap bobot limpa (P<0,05), tetapi berpengaruh tidak nyata terhadap bobot hati, jantung, bobot usus dan panjang usus (P>0,05). Penambahan infusa daun pandan wangi sampai 3% dalam air minum dapat ditolerir terhadap bobot hati, jantung, usus dan panjang usus broiler.
Historically Informed Soundscape: Mediating Past and Present
Authors: D. Linda Pearse  
Published: December 2024

Imagine for a moment that we engage with early-music manuscripts only through inspection of the musical text, without any attempt to perform it. Imagine again that musicians who perform early music made no attempt to engage with the historical context and performance practice of the past. It is mind-boggling to consider what we would miss if we did not make these attempts through performative artistic research. Can we engage with auditory culture over time by using methods from early-music performance practice to create soundscape? Will the process and product of creating a historically informed soundscape provoke new conversations and generate a new type of knowledge to engage both scholars of auditory culture and audiences? We want to complement scholarly narratives that seek to better understand the sounds of the past beyond music itself by borrowing methods and models from early-music performance and adapting them to create historically informed soundscape.
Plasma Proteomic Signatures of Adiposity Are Associated With Cardiovascular Risk Factors and Type 2 Diabetes Risk in a Multiethnic Asian Population
Authors: Lim Charlie G.Y. , Ozkan Bige , Liang Yujian , Chen Jingsha , Yao Jiali , Khaing Nang Ei Ei , Rooney Mary R. , Ndumele Chiadi E. , Tai E Shyong , Coresh Josef , Sim Xueling , van Dam Rob M. 
Published: December 2024

The biomarkers connecting obesity and cardiometabolic diseases are not fully understood. We aimed to 1) evaluate the associations between BMI, waist circumference (WC), and ∼5,000 plasma proteins (SomaScan V4), 2) identify protein signatures of BMI and WC, and 3) evaluate the associations between the protein signatures and cardiometabolic health, including metabolically unhealthy obesity and type 2 diabetes incidence in the Singapore Multi-Ethnic Cohort Phase 1 (MEC1). Among 410 BMI-associated and 385 WC-associated proteins, we identified protein signatures of BMI and WC and validated them in an independent data set across two time points and externally in the Atherosclerosis Risk in Communities (ARIC) study. The BMI and WC protein signatures were highly correlated with total and visceral body fat, respectively. Furthermore, the protein signatures were significantly associated with cardiometabolic risk factors and metabolically unhealthy obesity. In prospective analyses, the protein signatures were strongly associated with type 2 diabetes risk in MEC1 (odds ratio per SD increment in WC protein signature 2.84; 95% CI 2.47–3.25) and ARIC (hazard ratio 1.98; 95% CI 1.88–2.08). Our protein signatures have potential uses in the monitoring of metabolically unhealthy obesity.

Article Highlights
We evaluated the associations between ∼5,000 plasma proteins and BMI and waist circumference (WC) in a multiethnic Asian population. We identified 410 proteins associated with BMI and 385 proteins associated with WC and derived protein signatures of BMI and WC, which we validated externally in a U.S. cohort. Both the BMI and WC protein signatures were strongly associated with cardiometabolic risk factors, metabolically unhealthy obesity, and risk of obesity, metabolic syndrome, and type 2 diabetes. Our protein signatures have potential uses in monitoring metabolically unhealthy obesity.
Blocking Adipocyte YY1 Decouples Thermogenesis From Beneficial Metabolism by Promoting Spermidine Production
Authors: Qiu Chen , Lu Yu , Wu Suyang , Guo Wenli , Ni Jiahao , Song Jiyuan , Liu Zichao , Chang Xiaoai , Wang Kai , Sun Peng , Zhang Qian , Yang Shufang , Li Kai 
Published: December 2024

The accumulation of mitochondria in thermogenic adipose tissue (i.e., brown and beige fat) increases energy expenditure, which can aid in alleviating obesity and metabolic disorders. However, recent studies have shown that knocking out key proteins required to maintain mitochondrial function inhibits the energy expenditure in thermogenic fat, and yet the knockout (KO) mice are unexpectedly protected from developing obesity or metabolic disorders when fed a high-fat diet (HFD). In the current study, nonbiased sequencing-based screening revealed the importance of Yin Yang 1 (YY1) in the transcription of electron transport chain genes and the enhancement of mitochondrial function in thermogenic adipose tissue. Specifically, YY1 adipocyte-null (YAKO) mice showed lower energy expenditure and were intolerant to cold stress. Interestingly, YAKO mice showed alleviation of HFD-induced metabolic disorders, which can be attributed to a suppression of adipose tissue inflammation. Metabolomic analysis revealed that blocking YY1 directed glucose metabolism toward lactate, enhanced the uptake of glutamine, and promoted the production of anti-inflammatory spermidine. Conversely, blocking spermidine production in YAKO mice reversed their resistance to HFD-induced disorders. Thus, although blocking adipocyte YY1 impairs the thermogenesis, it promotes spermidine production, alleviates adipose tissue inflammation, and therefore leads to an uncoupling of adipose tissue energy expenditure from HFD-induced metabolic disorders.

Article Highlights
Chromatin open atlas profiling in white, beige, and brown adipocytes identified Yin Yang 1 (YY1) as a key transcription factor governing electron transport chain gene expression and mitochondrial function in thermogenic adipocytes. Knocking out adipocyte YY1 leads to impaired thermogenesis under cold stress while protecting the mice from diet-induced obesity and metabolic disorders. YY1-null adipocytes undergo metabolic reprogramming, with increased glutamine use and spermidine generation that combat adipose tissue inflammation and insulin resistance, resulting in an uncoupling of thermogenic capacity and metabolic benefits.
Effect of The Duration of The Diagnosis on DNA Methylation Changes in Three Different Diabetic Genes in Type 2 Diabetes Mellitus
Authors: Smail Harem Othman 
Published: December 2024

The primary epigenetic alteration is DNA methylation. This study examined variations in DNA methylation in patients with type 2 diabetes at various time intervals, concentrating on the Calpain-10 (CAPN10), ATP binding cassette subfamily C member 8 (ABCC8), and Transcription factor 7-like 2 (TCF7L2) genes. Since persistent diabetes can lead to aberrant methylation patterns, it is interested in how long these changes may last. The blood samples from 140 individuals with Type 2 Diabetes Mellitus (T2DM) were collected, and the patients were grouped according to how long they had been diagnosed. Four groups of individuals were created based on their time on the disease: those with T2DM <1 year, those with T2DM 1-3 years, and those with T2DM 3-5 years. The genders and ages of the participants were also noted. Using the Promega technique, bisulfite conversion and DNA extraction were completed. Methylation-specific PCR amplification was used to detect DNA methylation. In the study, ROC curve analysis, Chi-square and Spearman's correlation coefficients, as well as non-parametric tests, were employed to analyze the methylation percentage variation and methylation patterns among groups. A significant threshold of p < 0.05 was established. The results of the study indicated that the DNA methylation rates of T2DM patients and the control group differed significantly. Patients with type 2 diabetes, particularly those who had just received a diagnosis, showed higher levels of methylation than the control group. The study also found that the length of diagnosis may have an impact on the discriminative strength of the DNA methylation status of the ABCC8 gene, resulting in varying degrees of T2DM prediction.
ASPIRASI WARGA BINAAN TERHADAP PROGRAM TERPADU PENINGKATAN PERANAN WANITA MENUJU KELUARGA SEHAT SEJAHTERA
Authors: Novita Elvina 
Published: December 2024

Tujuan penelitian ini adalah (1) untuk mengetahui aspirasi warga binaan terhadap program terpadu peningkatan peranan wanita menuju keluarga sejahtera (2) mengetahui aspirasi positif yang merupakan keinginan warga masyarakat untuk meraih kemajuan di masa yang akan datang. Metode yang digunakan dalam penelitian ini adalah deskriptif asosiatif denga menggunakan metode survey untuk mendapatkan data dari tempat tertentu yang alamiah (bukan buatan) tetapi dengan melakukan perlakuan dalam pengumpulan data misalnya dengan mengedarkan kuesioner, test, wawancara terstruktur dan sebagainya. Penelitian korelasional yang merupakan bertujuan untuk mengetahui atau memperoleh informasi tentang hubungan variabel bebas dengan variabel terikatPopulasi dalam penelitian ini seluruh orangtua yang memiliki anak usia 15-18 tahun yang berjumlah 127, sampel 32 orang. Hasil penelitian ini menunjukkan terdapat hubungan yang positif dan signifikan antara sosial ekonomi orang tua dengan kelanjutan pendidikan anak di Desa Germenia Klambir Lima Kebun Kabupaten Deli Serdang dengan tingkat koefisien korelasi 0,970 yaitu termasuk dalam kategori sangat tinggi. Hal ini memperjelas bahwa semakin tinggi tingkat sosial ekonomi orang tua maka semakin besar peluang anaknya untuk melanjutkan pendidikan. Kata Kunci: anak, pendidikan, sosial ekonomi, orang tua
Tritrichomonas foetus is not located in the accessory glands, epididymis, or testicles of infected bulls, and post mortem changes influence recovery of organism
Authors: Koziol Jennifer H. , Roof Clinton J. , Myers Stephanie , Cruz Penn Michael , Williams Ryan B. 
Published: December 2024

Abstract

OBJECTIVE
To determine the presence of Tritrichomonas foetus in the accessory sex glands, epididymis, and testicular tissue of T foetus–positive bulls and to assess the impact of post mortem tissue acidity on quantitative reverse-transcription PCR results.

METHODS
8 bulls were euthanized from June 12 through June 15, 2023, and samples from their accessory sex glands, testicles, and epididymis were collected for T foetus testing. Preputial smegma was obtained before and after euthanasia and tested for T foetus by quantitative reverse-transcription PCR. The pH of the penile and preputial epithelial surfaces was measured.

RESULTS
Tritrichomonas foetus was not detected in samples from the testicle, epididymis, or epididymal semen. A post mortem decrease in the pH of the penis and prepuce correlated with a rise in cycle threshold values.

CONCLUSIONS
Tritrichomonas foetus was not present in samples taken, suggesting its absence in these specific reproductive tissues. A decrease in the pH of the penis and prepuce over time post mortem correlated with an increase in cycle threshold values in the samples. This relationship indicates that changes in pH may impact the detectability or stability of genetic material in these tissues, possibly affecting diagnostic outcomes as time from death progresses.

CLINICAL RELEVANCE
This research enhances the understanding of T foetus pathology in bulls and suggests that epididymal semen recovery could be a viable method for preserving genetic material from valuable bulls that test positive for T foetus. It also emphasizes the need for timely post mortem sampling to ensure accurate detection and management of T foetus in the cattle industry.
A CNN–LSTM-based deep learning model for early prediction of student’s performance
Authors: Arya Monika , Motwani Anand , Prasad Kauleshwar , Dewangan Bhupesh Kumar , Choudhury Tanupriya , Chauhan Piyush 
Published: December 2024

Abstract
In issues pertaining to higher education, deep learning (DL), and its connection to educational data, it is crucial to forecast students’ success. The ability to predict a student’s success aids in choosing courses and developing future study schedules. Apart from forecasting children’s performance, it also assists educators and administrators in keeping an eye on pupils, offering them support, and incorporating training initiatives to maximize outcomes. Student prediction has the advantage of lowering official warning flags and removing ineffective pupils from the classroom. By helping students select courses and study schedules that are suited for their skill levels, prediction supports the students directly. In the proposed approach, a methodology based on the integration of convolutional neural network (CNN) and long short-term memory (LSTM) is proposed to optimize students’ performance prediction systems. This study utilized a student performance dataset from the UCI ML Repository. It includes information on student achievements in secondary education from two Portuguese schools. The suggested method overcomes the following three problems in model development: an imbalanced dataset, a lack of feedback mechanism to enhance the quality of learning, and an inadequate mechanism to extract the learning patterns/relevant features to predict student performance. The suggested system’s effectiveness has been demonstrated by the accuracy (98.45) and loss (0.1989) obtained to achieve the best prediction.
Analysis of Academic Stress Factors and Their Impact on Academic Achievement of Mathematics Education Students
Authors: Yunarti Yuyun , Ikashaum Fertilia , Wulantina Endah , Mustika Juitaning , Sari Dwi Retno Puspita 
Published: December 2024

Changes in the learning system and high academic demands put pressure on first- and second-year bachelor's mathematics education students and, therefore, can affect students' academic achievement. The purpose of this study was to examine academic stress factors and their impact on the educational achievement of mathematics education students in 80 respondents using a descriptive quantitative approach. This study discusses adolescent academic stress factors and covers students' physical and psychological well-being. The data obtained were analyzed using factor analysis. Exploratory factor analysis extracted 30 daily stress factors of students into three specific stress factors: stress on lecture material, environment, and time management. The results of this study indicate that students have moderate levels of stress. Then, Pearson analysis was conducted to see the correlation between stress factors and cumulative grade point average. The results showed that the three factors did not significantly correlate to grades with a correlation power below 0.5. This means that students can manage their academic stress well. Student stress management approaches can focus on each factor separately, depending on individual needs. Internal and external support, strengthening academic literacy, improving conducive learning environment facilities, relevant skills development programs, and strengthening social networks can help reduce student stress.
ASSESSMENT OF PEATLAND ECOSYSTEM CHARACTERISTICS IN THE PEAT HYDROLOGICAL UNIT OF GONGAN RIVER- NILO RIVER, RIAU PROVINCE
Authors: Susetyo Budi , Waluyo Waluyo , Hermawan Erwin 
Published: December 2024

Minister of Environment and Forestry Regulation No. P.14/MENLHK/SETJEN/KUM.1/2/2017 establishes protocols for assessing and defining the function of peat ecosystems, involving 13 criteria. Among these, survey points must align with map coordinates, with a displacement limit of 200 meters. The research aims to meticulously control the characteristics of peatlands in the Peat Hydrological Unit (PHU) of Gongan River-Nilo River, Riau Province, following the work plan to ensure that observations align with actual conditions. The Haversine method gauges the distance between planned and actual observation points, revealing an average shift of 19.9 meters, ranging from 3.38 to 198.9 meters, indicating surveyor accountability. Field data shows 68% of the area with groundwater levels below 30 cm, and 32% between 30 and 60 cm. Channel water heights are predominantly between 50 and 100 cm (68%), with 22% lower and 10% higher. Soil pH consistently measures below 4 (100%), similar to channel water (73%), and substratum (81%) with most below 4. Only 3.1% of groundwater exceeds 300 μS/cm in electrical conductivity, while 96% are below 300 μS/cm, including low substratum conductivity. Total dissolved solids are mostly below 75 ppm (97%), with 3% between 75 and 150 ppm. The average peat thickness is 367.9 cm, primarily clay/river sediment at the hemic level. It can be concluded that all field survey results in the PHU Gongan-Nilo River can be accepted as valid data that can be used as a basis for development planning/regional determination in this area.
Evaluation of Syndecan-1 Expression in Iraqi Patients with Papillary Thyroid Carcinoma
Authors: Al-Timimi Noor Mohammed Naji , Baraaj Abed Hassan 
Published: December 2024

Papillary thyroid carcinoma (PTC) represents the most prevalent kind of thyroid gland cancer, making up around 80% of all occurrences of thyroid cancer. Evidence shows that Syndecan-1 (SDC-1) expression is lost in a number of benign and malignant epithelial neoplasms, although its expression profile in thyroid gland neoplasms is yet unknown. Therefore, the aim of this study was to assess SDC-1 expression in papillary thyroid carcinoma patients, as well as the relationship between age and gender and SDC-1 expression. To undertake a detailed investigation of SDC-1 in normal and malignant tissues, tissue sections were used to examine SDC-1 expression in 70 tissue samples, 50 distinct PTC (6 males and 44 females) and 20 normal tissue types (10 from each gender) as control. Ages in each group ranged from 20 to 60 years. The results showed a statistically different level of SDC-1 expression in patients and control. There was no significant relationship between SDC-1 expression and gender or age, in accordance with recent research findings which suggest that SDC- 1 might be exploited as a prognostic indicator or a target in cancer treatment in the future.