bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oxidized products ofα-linolenic acid negatively regulate cellular survival and motility of breast cancer cells
Authors: Gutierrez-Pajares Jorge L. , Hassen Celine Ben , Oger Camille , Galano Jean-Marie , Durand Thierry , Frank Philippe G. 
Published: January 2019

AbstractBackgroundCancer is a major cause of death in the world, and more than six million new cases are reported every year. Despite recent advances in our understanding of the biological processes leading to the development and progression of cancer, there is still a need for new and effective agents to treat this disease. Phytoprostanes (PhytoPs) and phytofurans (PhytoFs) are non-enzymatically oxidized products of α-linolenic acid that are present in seeds and vegetable oils. They have been shown to possess anti-inflammatory and apoptosis-promoting activities in macrophages and leukemia cells, respectively.MethodsIn this work, seven PhytoPs (PP1-PP7) and one PhytoFs (PF1) were evaluated for their cytotoxic, chemosensitization and anti-migratory activities using the MCF-7 and MDA-MB-231 breast cancer cell lines.ResultsAmong the compounds tested, only three PhytoPs had a significant effect on cell viability compared to the control group:Ent-9-L1-PhytoP (PP6) decreased cell viability in both cell lines, while 16-F1t-PhytoP (PP1) and 9-L1-PhytoP (PP5) decreased viability in MCF-7 and MDA-MB-231 cells, respectively. When combined with a sub-cytotoxic dose of doxorubicin, these three PhytoPs significantly enhanced the cytotoxic effect on MCF-7 cells while the chemotherapeutic drug alone had no effect. In cellular motility assays,Ent-9-(RS)-12-epi-ST-Δ10-13-PhytoF could significantly inhibit cellular migration of MDA-MB-231 cells in a wound-healing and a transwell assays. In addition,Ent-9-(RS)-12-epi-ST-Δ10-13-PhytoF also enhanced cellular adhesion of MDA-MB-231 cells.ConclusionsThis study shows for the first time that the plant-derived compounds PhytoPs and PhytoFs could be further exploited alone or in combination with chemotherapy to improve the arsenal of therapies available against breast cancer.
A novel role for the tumor suppressor geneITF2in lung tumorigenesis and chemotherapy response
Authors: Pernía Olga , Sastre-Perona Ana , Rodriguez-Antolín Carlos , García-Guede Alvaro , Palomares-Bralo María , Rosas Rocío , Sanchez-Cabrero Darío , Cruz Patricia , Rodriguez Carmen , Diestro MDolores , Martín-Arenas Rubén , Pulido Verónica , Santisteban Pilar , de Castro Javier , Vera Olga , Ibáñez de Cáceres Inmaculada 
Published: January 2019

SUMMARYDespite often leading to platinum resistance, platinum-based chemotherapy continues to be the standard treatment for many epithelial tumors. In this study we analyze the cytogenetic alterations that arise after cisplatin treatment providing novel insights into the molecular biology and the cellular mechanisms involved in the acquired resistance in these tumor types.MethodsIn this study, we used 1 million array-CGH and qRT-PCR methodologies to identify and validate cytogenetic alterations that arise after cisplatin treatment in four lung and ovarian paired cisplatin-sensitive/resistant cell lines. We used whole transcriptome sequencing (RNA-seq), functional transfection assays and gene-pathway activity analysis in our experimental cellular models and in fresh frozen primary NSCLC tumors to identify genes with a potential role in the development of this malignancy. Results were further explored in 55 lung and ovarian primary tumors and control samples and in two extensive in silico databases (TCGA and KMplotter) with 1,926 NSCLC and 1,425 additional epithelial tumors.ResultsLong-term cell exposure to platinum induces the frequent deletion ofITF2gene. Restoration ofITF2expression re-sensitizes tumor cells to platinum and recovers the levels of Wnt/β-catenin transcriptional activity.ITF2expression was also frequently downregulated in NSCLC, ovarian and other epithelial tumors, predicting a worse overall survival. We also identified an inverse correlation in expression betweenITF2andHOXD9, revealing that NSCLC patients with lower expression ofHOXD9have a better overall survival rate that was independent of the tumor histology.ConclusionWe have defined the implication ofITF2as a molecular mechanism behind the development of cisplatin resistance probably through the activation of the Wnt-signaling pathway. Our translational data suggest thatITF2could be used as a general epithelial tumor platinum-predictive marker and have identifiedHOXD9as a potential prognostic biomarker in NSCLC, a gene which expression is induced byWntsignaling. Furthermore, this data highlights the possible role ofITF2andHOXD9as a novel therapeutic target for platinum resistant tumors.
Stem-cell-ubiquitous genes spatiotemporally coordinate division through regulation of stem-cell-specific gene networks
Authors: Clark Natalie M , Buckner Eli , Fisher Adam P , Nelson Emily C , Nguyen Thomas T , Simmons Abigail R , de Luis Balaguer Maria A , Butler-Smith Tiara , Sheldon Parnell J , Bergmann Dominique C , Williams Cranos M , Sozzani Rosangela 
Published: January 2019

AbstractStem cells are responsible for generating all of the differentiated cells, tissues, and organs in a multicellular organism and, thus, play a crucial role in cell renewal, regeneration, and organization. A number of stem cell type-specific genes have a known role in stem cell maintenance, identity, and/or division. Yet, how genes expressed across different stem cell types, referred here as stem-cell-ubiquitous genes, contribute to stem cell regulation is less understood. Here, we find that, in the Arabidopsis root, a stem-cell-ubiquitous gene, TESMIN-LIKE CXC2 (TCX2), controls stem cell division by regulating stem cell-type specific networks. Development of a mathematical model of TCX2 expression allowed us to show that TCX2 orchestrates the coordinated division of different stem cell types. Our results highlight that genes expressed across different stem cell types ensure cross-communication among cells, allowing them to divide and develop harmonically together.
The Relationship between Parasitic Fleas and Small Mammals in Household of Western Yunnan Province, China
Authors: Yin Jia-Xiang , Cheng Xiao-Ou , Zhao Qiu-Fang , Wei Zhao-Fei , Xu Dan-Dan , Wang Meng-Di , Zhou Yun , Wang Xiu-Fang , Liu Zheng-Xiang 
Published: January 2019

AbstractThe Yunnan Province has one of the most serious outbreaks of the plague epidemic in China. Small mammals and fleas are risk factors for the occurrence of plague in commensal plague foci. Understanding the relationship between parasitic fleas and small mammals will help control fleas and prevent the onset of the plague. Four hundred and twenty-one small mammals, belonging to 9 species, were captured. Of these, 170 small mammals (40.4%) were infested with fleas. A total of 992 parasitic fleas (including 5 species) was collected. The number of Leptopsylla Segnis and Xenopsylla Cheopis was 91.0%. The final multiple hurdle negative binomial regression model showed that when compared with Rattus Tanezumi, the probability of flea infestation on Mus musculus and other host species decreased from 58% to 99%, while the infestation with fleas from other host species increased 4.7 fold. The probability of flea prevalence in adult hosts increased by 74%, while the number of fleas decreased by 76%. The number of flea infestations in small male mammals increased by 62%. The number of fleas in small mammals weighing more than 59 grams has been multiplied by about 4. Rattus Tanezumi is the predominant species in households in West Yunnan Province, while Leptopsylla Segnis and Xenopsylla Cheopis are dominant parasitic fleas. There is a strong relationship between the abundance of parasitic fleas and the characteristics of small mammals (e.g. Species, age, sex, and body weight).
E6 proteins from high-risk HPV, low-risk HPV, and animal papillomaviruses activate the Wnt/β-catenin pathway through E6AP-dependent degradation of NHERF1
Authors: Drews Camille M. , Case Samuel , Vande Pol Scott B. 
Published: January 2019

AbstractHigh-risk human papillomavirus (HPV) E6 proteins associate with the cellular ubiquitin ligase E6-Associated Protein (E6AP), and then recruit both p53 and certain cellular PDZ proteins for ubiquitination and degradation by the proteasome. Low-risk HPV E6 proteins also associate with E6AP, yet fail to recruit p53 or PDZ proteins; their E6AP-dependent targets have so far been uncharacterized. We found a cellular PDZ protein called Na+/H+ Exchanger Regulatory Factor 1 (NHERF1) is targeted for degradation by both high and low-risk HPV E6 proteins as well as E6 proteins from diverse non-primate mammalian species. NHERF1 was degraded by E6 in a manner dependent upon E6AP ubiquitin ligase activity but independent of PDZ interactions. A novel structural domain of E6, independent of the p53 recognition domain, was necessary to associate with and degrade NHERF1, and the NHERF1 EB domain was required for E6-mediated degradation. Degradation of NHERF1 by E6 activated canonical Wnt/β-catenin signaling, a key pathway that regulates cell growth and proliferation. Expression levels of NHERF1 increased with increasing cell confluency. This is the first study in which a cellular protein has been identified that is targeted for degradation by both high and low-risk HPV E6 as well as E6 proteins from diverse animal papillomaviruses. This suggests that NHERF1 plays a role in regulating squamous epithelial growth and further suggests that the interaction of E6 proteins with NHERF1 could be a common therapeutic target for multiple papillomavirus types.Author summaryPapillomaviruses cause benign squamous epithelial tumors through the action of virally encoded oncoproteins termed E6 and E7, which are classified as either high or low-risk based upon the propensity of the tumor to evolve into cancer. E6 proteins from both high and low-risk HPVs interact with a cellular ubiquitin ligase called E6AP. High-risk E6 proteins hijack E6AP ubiquitin ligase activity to target p53 for degradation. Degradation targets of the low-risk E6 proteins in complex with E6AP have not been described. Here, we describe a protein called NHERF1 that is targeted for degradation by both high and low-risk E6 proteins, as well as E6 proteins from diverse animal species. Degradation of NHERF1 resulted in activation of an oncogenic cellular signaling pathway called Wnt. Identification of NHERF1 as a highly conserved E6 degradation target could inform therapies directed against both low-risk HPVs and cancer-inducing high-risk HPVs.
On the hot-rolled recycled carbon steels: their oxide formation, pickling ability and scale adhesion
Authors: Chandra-ambhorn Somrerk , Tungtrongpairoj Jennarong , Jutilarptavorn Anuwat , Nilsonthi Thanasak , Somphakdee Tanongsak 
Published: January 2019

Purpose
The purpose of this study is to investigate the formation, pickling ability and adhesion of thermal oxide scales on the hot-rolled recycled steels produced from the medium and thin slabs. Because the scale on the steel produced from the medium slab was relatively thick of about 11 µm, it contained cracks after hot-rolling. Thus during pickling, the scale was uniformly attacked with the simultaneous dissolution of the inner scale because of the penetration of acid through cracks. However, the scale on the steel produced from the thin slab was thinner of about 6 µm and thus, nearly crack-free. The pickling solution thus attacked the scale surface uniformly. At longer pickling periods, pits were also nucleated and propagated. Concurrently, the tensile testing machine with a CCD camera has been applied to observe scale adhesion.

Design/methodology/approach
The formation, pickling ability and adhesion of thermal oxide scales on the hot-rolled recycled steels produced from the recycled slab, e.g. medium slab and thin slab, were investigated. The morphology and phase identification were examined by using scanning electron microscopy, X-ray diffraction and Raman spectroscopy. Furthermore, the adhesion behaviour of oxide scale was investigated by immersion test and tensile test with a CCD camera.

Findings
For the scale formation, it was found that the hematite and magnetite were formed on the hot-rolled recycled steels produced from the medium and thin slabs. For the immersion test, it was found that the scale on hot-rolled recycled steels produced from the medium slab was more difficult to be pickled as represented by the longer time for the complete pickling. This was consistent with the result of tensile test; the steel produced from the medium slab had better scale adhesion as represented by the higher strain initiating the first spallation of scale.

Originality/value
The effects of slab types and its alloying element were investigated to understand the scale adhesion behaviour. The empirical pickling mechanisms and the mechanical adhesion energy were proposed. It led to the understanding in the control of alloying element in the hot-rolled steel.
The role of central government and local government and the moderating effect of good governance on forest fire policy in Indonesia
Authors: Brotestes Panjaitan Raffles , Sumartono Sumartono , Sarwono Sarwono , Saleh Choirul 
Published: January 2019

Purpose
The purpose of this paper is to investigate forest fires and their relationship to prevention and mitigation strategies based on the empirical problems raised by this study. Public policy implementation (in this case, the policy of forest fire management) is influenced by the role played by government and by the participation of the public and stakeholders (in this case, companies), as well as the effects of good governance. Thus, from the empirical problems associated with theoretical problems and normative problems, this study raises the influence of the role of central and local government on the implementation of forest fire prevention policy in Indonesia, which is moderated by the good governance variable.

Design/methodology/approach
This study used a quantitative approach by adopting survey methodology. The study has aimed to assess both large and small population groups, by selecting and reviewing carefully chosen samples of the population to find the incidence, distribution and relative interrelation of the variables considered (Kerlinger and Lee, 2000). The survey was undertaken in areas of Indonesia that have a high level of vulnerability to forest fires. There are currently six provinces – Riau, Jambi, South Sumatra, West Kalimantan, East Kalimantan and South Kalimantan – that have the highest intensity of forest fires. The study population was taken from 105 villages in those six major provinces experiencing forest fires. Sample size precision was determined by using Slovin’s formula with a precision of 10 percent and, thus, a sample size of 52 was obtained.

Findings
The central government’s activities have no significant effect on regional forest fire prevention. However, the results found that there is a significant effect caused by the interaction between the central and local governments and their governance of forest fire prevention. Even though the direct effect is not significant, the interaction effect significantly influences the forest fire prevention governance variable, which is a pure moderator. This study found that the role of central government has no effect on forest fire prevention. If the role of the central government is high, it will not impact the effectiveness of forest fire prevention, which is reflected in the aspects of prevention and early warning, reward and punishment, the improvement and management of ecosystems by reviewing courts, law enforcement and national and regional synergy.

Originality/value
This is one of the few public administration science studies to have investigated the relationship between good governance and forest fire policy in Indonesia, particularly the combined roles played by central and local governments.
The content disseminated on social media by public secondary school libraries as a reflection of society
Authors: Faba-Pérez Cristina , Infante-Fernández Lara María 
Published: January 2019

Purpose
The purpose of this paper is to analyze the type of content disseminated by school libraries through social media and what topics are the most commonly used, to discover if, in addition to topics concerning libraries, information with a wide social scope is also disseminated.

Design/methodology/approach
This paper reviews the use of social media as well as the implementation of a content analysis on the most used 2.0 platforms, to locate which content is the most relevant in the school libraries of public secondary schools in Extremadura.

Findings
In the Extremadura region of Spain, the results of the 752 publications posted during the period 2014-2017 by the libraries of the 86 public secondary schools on six selected social media platforms generated a total of 4 categories and 14 subcategories, and point to a predominance of topics related to encouraging and promoting reading and writing, and to the library’s support function for both the classroom and the school. However, shortcomings are detected in content related to social aspects of special interest, such as bullying or education in equality.

Originality/value
The originality of this paper lies in the fact that although there are some works on the analysis of the content of the social web of libraries in general, especially university libraries, the same does not apply to the evaluation of social media in school libraries, and much less about the analysis of social media content in these types of libraries.
Governing major transformation portfolios in practice: illustrations from the UK central government
Authors: Kortantamer Dicle 
Published: January 2019

Purpose
The purpose of this paper is to examine major transformation portfolio governing practices. Previous research focuses on the structure of project portfolio governance and is relatively silent on micro-practices of governing. This paper intends to respond to this gap.

Design/methodology/approach
This qualitative case study draws upon practice approaches and the theoretical lens of organisational routines. The empirical inquiry is conducted across six major transformation portfolios in two UK central government departments. The data are collected through conducting semi-structured interviews and gathering documentary reports and analysed by adopting an abductive approach.

Findings
The findings highlight that two governing practices that have not been previously discussed in the literature: structuring the policy relationship and structuring the business relationship. These practices can be associated with the complex ownership system of the UK central government. The findings also show that in complex ownership systems owners may have different governing and supporting orientations. The micro-dynamics of these governing practices illustrate that the ecology of governing practices is complex, both in terms of the co-existence of complementary and competitive relationships between practices, and in terms of bundles of practices enacting different control regimes.

Research limitations/implications
This qualitative case study supports further inquiry into major transformation portfolio governing and complex ownership systems.

Originality/value
The paper adopts a governance-as-practice approach and examines governing major transformation portfolios.
Maintenance performance improvement analysis using Fuzzy Delphi method
Authors: Ghazali Zulkipli , Lim M. Ridhuan Tony , A. Jamak Abu Bakar Sedek 
Published: January 2019

Purpose
The purpose of this paper is to analyze issues pertaining to maintenance performance and to develop a framework that addresses challenges of maintenance management of an international lube blending plant in Malaysia. This study capitalizes on the contribution of selected maintenance department stakeholders from within the plant to develop “tailor-made” intervention strategies for maintenance performance improvement.

Design/methodology/approach
The study employed two focus group workshops to ascertain issues facing the maintenance department and identify intervention strategies. The final phase of the study employed fuzzy Delphi method (FDM) to prioritize and rank the intervention strategies for performance improvement.

Findings
The first focus group workshop identified 106 issues which could be classified under aspects of spare parts (n=8), equipment (n=14), communication (n=12), non-technical resource (n=8), health, safety and environment (n=4), technical skills and recruitment (n=27), and handling and procedures (n=33). Based on these findings, the second focus group revealed 28 significant performance initiatives to improve the issues identified for maintenance performance improvement. Through the FDM, 18 performance initiatives were ranked and prioritized. Performance improvement through leadership category leads the overall initiatives followed by equipment maintenance management, talent management, work environment and vendor management.

Research limitations/implications
Interesting implications for maintenance management theory would be realized if future research were able to demonstrate that certain aspects or dimensions were related to high performing plant maintenance, and not with low performing ones. Apparently, the present study is not able to provide this clue because it is merely a case study of a single company.

Practical implications
As ILBP attempts to implement maintenance performance improvement, it is pertinent for the management to understand the relevant performance issues and concerns. The appropriate enablers have been identified and must be initiated to chart the strategic role of their maintenance organization.

Social implications
This study contributes toward further understanding of the maintenance performance management. It has demonstrated the need of organizations to make infrastructural investments such as quality leadership, employee training and empowerment, to name a few, to strategically enhance their maintenance capabilities.

Originality/value
This study uses the FDM for the decision-making process of improving plant maintenance performance. It adds value to the body of knowledge of plant operation management.