bluuebunny/crossref_metadata_2025_split huggingface dataset:
DIVERSIFIKASI DAN KARAKTERISASI CITARASA BAKSO IKAN TENGGIRI (Scomberomus commerson) DENGAN PENAMBAHAN ASAP CAIR TEMPURUNG KELAPA
Authors: Parnanto Nur Her Riyadi , Atmaka Windi 
Published: July 2019

<span class="fontstyle0">Kecenderungan konsumen terhadap produk pangan bercitarasa asap semakin meningkat. Bahkan akhir-akhir ini<br />muncul produk pangan bercitarasa asap yang dahulu tidak lazim diperdagangkan seperti nasi bakar, tahu bakar<br />dan kelapa bakar. Namun, pengasapan cara langsung memungkinkan terbentuknya senyawa karsinogenik.<br />Penggunaan asap cair merupakan salah satu alternatif untuk memperbaiki proses tersebut sehingga dihasilkan<br />produk pangan bercitarasa asap yang aman bagi kesehatan. Ikan tenggiri mempunyai tingkat produksi dan<br />kandungan gizi yang cukup tinggi. Bakso ikan merupakan salah satu bentuk diversifikasi produk perikanan.<br />Bakso merupakan salah satu produk yang paling digemari. Namun, sejauh ini citarasa bakso masih standar<br />sehingga berpotensi untuk dikembangkan. Salah satunya adalah dengan cara menambahkan citarasa asap pada<br />produk bakso ikan tenggiri.<br />Tujuan penelitian ini adalah untuk mengetahui metode dan konsentrasi pemberian asap cair pada bakso ikan<br />tenggiri yang memiliki tingkat kesukaan paling tinggi, mengetahui karakter sensoris (warna, aroma asap, rasa<br />asap, kekenyalan dan keseluruhan) serta untuk mengetahui karakter fisik (tekstur) dan karakter kimia (kadar air,<br />lemak, protein dan fenol) terhadap bakso ikan tenggiri citarasa asap yang paling disukai. Rancangan yang<br />digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor yaitu variasi metode<br />penambahan asap cair (pencampuran, pencelupan dan penyemprotan) serta variasi konsentrasi asap cair (3%, 5%<br />dan 7%). Analisis data secara statistik dengan ANOVA pada α=5% serta dilanjutkan dengan uji DMRT apabila<br />ada beda nyata.<br />Hasil penelitian menunjukkan bahwa variasi metode serta konsentrasi pemberian asap cair pada bakso ikan<br />tenggiri tidak memberikan pengaruh beda nyata terhadap sifat sensori warna, kekenyalan serta keseluruhan tetapi<br />memberikan pengaruh bervariasi terhadap sifat sensori aroma asap dan rasa asap. Bakso ikan tenggiri dengan<br />metode penyemprotan asap cair 3% merupakan metode serta konsentrasi pemberian asap cair yang memiliki<br />tingkat kesukaan paling tinggi. Karakteristik kimia bakso ikan tenggiri dengan penyemprotan asap cair 3% telah <span class="fontstyle0">sesuai dengan persyaratan SNI bakso ikan yaitu: kadar air 75,06%, kadar protein 10,16% dan kadar lemak<br />0,86%. Mengandung fenol alami sebesar 0,07061% dan masih jauh di bawah batas maksimal kadar fenol yang<br />diperbolehkan dalam bahan makanan (0,02-0,1%) sehingga aman untuk dikonsumsi serta dapat diterima<br />konsumen dari karakter sensoris, fisik dan kimia.</span> </span>
Adenocarcinoma with mixed subtypes is a rare but aggressive histologic subtype in colorectal cancer
Authors: Sheng Hui , Wei Xiaoli , Mao Minjie , He Jincan , Luo Tianqi , Lu Shilin , Zhou Liye , Huang Zhixin , Yang Anli 
Published: July 2019

Abstract
Background: Though there have been studies investigating the clinicopathologic and prognostic relevance of mucinous adenocarcinoma (MAC) and signet-ring cell carcinoma (SRCC) compared with classic adenocarcinoma (CA), little is known about the prognosis of adenocarcinoma with mixed subtypes (AM) and the differences among these four subtypes.

Methods: The statistics of colorectal cancer registered in the Surveillance, Epidemiology and End Results (SEER) database were retrieved and analyzed. We also compared the clinicopathologic and prognostic relevance between CA, SRCC, MAC and AM.

Results: The frequencies of these four subtypes were 69.9% (CA, n=15,812), 25.1% (MAC, n=5,689), 3.6% (SRCC, n=814) and 1.4% (AM, n=321). All of MAC, SRCC and AM were significantly related with aggressive features. Only SRCC and AM were independent poor prognostic markers for overall survival by multivariate analysis. The malignancy degree of AM was between MAC and SRCC according to the clinicopathologic associations. The prognosis of AM was significantly worse than MAC but comparable with SRCC.

Conclusions: We confirmed the clinicopathologic relevance with aggressive features of MAC and SRCC, as well as the poor prognostic relevance of SRCC by analyzing a large study population. Furthermore, we identified AM as a rare but aggressive histologic subtype in colorectal cancer, which particular attention should be given in clinical practice.
MPPa-PDT suppresses breast tumor migration/invasion through inhibiting AKT-NF-κB-dependent MMP-9 expressions via ROS
Authors: Huang Liyi , Lin Haidan , Chen Qing , Yu Lehua , Bai Dingqun 
Published: July 2019

Abstract
Background: breast cancer is the most commonly women cancer and most breast cancer deaths are related to tumor metastasis. Therefore, inhibiting metastasis may provide a therapeutic treatment for breast cancer. In the present study, pyropheophorbide-α methyl ester mediated photodynamic therapy (MPPa-PDT) was used to inhibit metastasis in breast cancer cells MCF-7. Methods: Uptake of MPPa was detected by fluorescence microscope. Cell viability was evaluated by CCK-8. Generation of ROS were detected by DCFH-DA. Migration of cells was assessed by wound healing assay and invasion ability was assessed by Matrigel invasion assay. Levels of MMP2 and MMP9 were measured by PCR. Akt, Phospho-Akt, Phospho-NF-kB p65 and NF-kB p65 were measured by western blotting. F-actin cytoskeleton was observed by immunofluorescence. Lung organs were stained with Hematoxylin and Eosin. Results: Following MPPa-PDT, migration and invasion were decreased in the MCF-7 cells. MPPa-PDT down-regulated expression of MMP2 and MMP9 which is responsible for metastasis. MPPa-PDT reduced the phosphorylation of AKT and NF-κB. MPPa-PDT also destroyed cytoskeleton F-actin in MCF-7 cells. These effects were blocked by the reactive oxygen scavenger NAC or AKT activator SC79 while PI3K inhibitor LY294002 or AKT inhibitor Triciribine increased these effects. Moreover, MPPa-PDT inhibited tumor metastasis and destroyed F-actin in vivo. Conclusion: taken together, these results demonstrated that MPPa-PDT inhibits metastasis of MCF-7 cells both in vitro and vivo, and that may involve in AKT-NF-κB-dependent MMP-9 signaling pathway. Thus, MPPa-PDT may be a promising therapeutic treatment to inhibit metastasis.
Rapid identification and characterization of genetic loci for defective kernel in bread wheat
Authors: Fu Chao , Du Jiuyuan , He Zhonghu , Tian Xiuling , Wang Yue , Fu Luping , Xu Xiaoting , Xu Dengan , Xia Xianchun , Zhang Yan , Cao Shuanghe 
Published: July 2019

Abstract
Background

Wheat is a momentous crop and feeds billions of people in the world. The improvement of wheat yield is very important to ensure world food security. Normal development of grain is the essential guarantee for wheat yield formation. The genetic study of grain phenotype and identification of key genes for grain filling are of great significance upon dissecting the molecular mechanism of wheat grain morphogenesis and yield potential.

Results

Here we identified a pair of defective kernel (Dek) isogenic lines, BL31 and BL33, with plump and shrunken mature grains, respectively, and constructed a genetic population from the BL31/BL33 cross. Ten chromosomes had higher frequency of polymorphic single nucleotide polymorphism (SNP) markers between BL31 and BL33 using Wheat660K chip. Totally 783 simple sequence repeat (SSR) markers were chosen from the above chromosomes and 15 of these were integrated into two linkage groups using the genetic population. Genetic mapping identified three QTL, QDek.caas-3BS.1, QDek.caas-3BS.2 and QDek.caas-4AL, explaining 14.78 - 18.17%, 16.61 - 21.83% and 19.08 - 28.19% of phenotypic variances, respectively. Additionally, five polymorphic SNPs from Wheat660K were successfully converted into cleaved amplified polymorphic sequence (CAPS) markers and enriched the target regions of the above QTL. Biochemical analyses revealed that BL33 has significantly higher grain sucrose contents at filling stages and lower mature grain starch contents than BL31, indicating that the Dek QTL may be involved in carbohydrate metabolism. As such, the candidate genes for each QTL were determined according to International Wheat Genome Sequence Consortium (IWGSC) RefSeq v1.0.

Conclusions

Overall, we achieved a rapid QTL mapping and reasonable predication of causal genes for Dek, laying foundation to conduct fine mapping and dissect the regulatory mechanism underlying Dek trait in wheat.
Comparative transcriptome analysis between resistant and susceptible tomato lines uncovers the response mechanism of Cf-16-mediated resistance to Cladosporium fulvum
Authors: Zhang Dongye , Yang Huanhuan , Xu Xiangyang 
Published: July 2019

Abstract
Background

Leaf mold disease caused by Cladosporium fulvum is a major disease in cultivated tomato plants and affects global tomato production. Some Cf genes, of which Cf-16 is an effective gene for resisting tomato leaf mold, are associated with leaf mold resistance; however, the molecular mechanism is largely unknown.

Results

we used comparative transcriptome analysis of C. fulvum-resistant (cv. Ontario7816, including the Cf-16 gene) and C. fulvum-susceptible (cv. Moneymaker) tomato lines to identify differentially expressed genes (DEGs) at 4 and 8 days postinfection with C. fulvum. We found that the number of DEGs in the Cf-16 tomatoes was significantly higher than the number of DEGs in the Moneymaker tomatoes. In addition, 1,350 DEGs were shared among Cf-16 groups at 4 and 8 dpi, suggesting the existence of a common core of DEGs in response to C. fulvum infection. Upregulated DEGs were mainly associated with defense processes and phytohormone signaling, including salicylic acid (SA) and jasmonic acid (JA), in the Cf-16 tomato. Moreover, the SA and JA contents significantly increased in the Cf-16 tomato at the early stages of C. fulvum infection. Comprehensively, more upregulated DEGs were found in the Cf-16 tomato than in the Cf-10 and Cf-12 tomatoes at the early stage of C. fulvum infection. However, the significantly enriched defense-signaling pathways involved in Cf-16 had some distinctions from those in Cf-10 and Cf-12.

Conclusion

Our results provide new insights into the resistance response mechanism of Cf genes to C. fulvum, especially the unique characteristics of Cf-16 in response to C. fulvum infection.
Body mass index and lifetime healthcare utilization
Authors: Edwards Christina Hansen , Aas Eline , Kinge Jonas Minet 
Published: July 2019

Abstract
Background: Overweight and obesity is a major global public health challenge, and understanding the implications for healthcare systems is essential for policy planning. Past studies have typically found positive associations between obesity and healthcare utilization, but these studies have not taken into consideration that obesity is also associated with early mortality. We examined associations between body mass index (BMI, reported as kg/m2) and healthcare utilization with and without taking BMI-specific survival into consideration.

Methods: We used nationally representative data on 33 882 adults collected between 2002 and 2015. We computed BMI- and age-specific primary and secondary care utilization and multiplied the estimated values with gender-, age-, and BMI-specific probabilities of surviving to each age. Then, we summed the average BMI-specific utilization between 18 and 85 years.

Results: During a survival-adjusted lifetime, males with normal weight (BMI: 18.5–24.9) had, on average, 167 primary care, and 77 secondary care contacts. In comparison, males with overweight (BMI: 25.0–29.9), category I obesity (BMI: 30.0–34.9), and category II/III obesity (BMI ≥35.0) had 11%, 41%, and 102% more primary care, and 14%, 29%, and 78% more secondary care contacts, respectively. Females with normal weight had, on average, 210 primary care contacts and 91 secondary care contacts. Females with overweight, category I obesity, and category II/III obesity had 20%, 34%, and 81% more primary care contacts, and 26%, 16%, and 16% more secondary care contacts, respectively.

Conclusion: The positive association between BMI and healthcare utilization was reduced, but not offset, when BMI-specific survival was taken into consideration. Our findings underpin previous research and suggest that interventions to offset the increasing prevalence of overweight, and especially obesity, are warranted.
Knowledge and attitudes among health professionals about poliomyelitis and post-poliomyelitis syndrome: a cross-sectional study in Brazil
Authors: Lira Claudio Andre Barbosa de , Santos Douglas Assis Teles , Viana Ricardo Borges , Guimarães Juliana Moreira , Oliveira Jéssica Nathalia Soares , Sousa Bolivar Saldanha , Santana Marcos Gonçalves , Vancini Rodrigo Luiz , Andrade Marília Santos 
Published: July 2019

Abstract
Background

Post-poliomyelitis syndrome is a clinical condition that can affect poliomyelitis survivors. Our aim was to evaluate post-poliomyelitis syndrome knowledge among Brazilian health professionals.

Methods

A self-administered questionnaire (30 questions) was designed to probe knowledge about poliomyelitis and post-poliomyelitis syndrome. From the questionnaire, we created a structured exam to evaluate the performance of the professionals. The exam was composed of 20 questions and a score was provided, varying from 0 (totally ill advised) to 20 (totally well-informed).

Results

In general, physicians, physiotherapists and nurses demonstrated a better understanding of poliomyelitis and post-polio syndrome. The health professionals who had received previous information about poliomyelitis and PPS had significantly higher scores than those that had never received information (p<0.001). On average, this difference was approximately 28.6%.

Conclusions

The findings of the present study indicate a critical need for the improvement of PPS knowledge among Brazilian health professionals.
Pulmonary Metastasectomy in Colorectal Cancer (PulMiCC): a multi-centre randomized clinical trial
Authors: Treasure Tom , Farewell Vern , Macbeth Fergus , Monson Kathryn , Williams Norman R , Graves Chris-Brew , Lees Belinda , Grigg Olivia , Fallowfield Lesley 
Published: July 2019

Abstract
Background: Lung metastasectomy in the treatment of advanced colorectal cancer has been adopted and established without control data. Our aim was to test its effectiveness in a randomised trial.

Methods: Multidisciplinary teams in 13 hospitals recruited participants with potentially resectable lung metastases to a multicentre 2-arm randomised trial comparing active monitoring with or without metastasectomy. Other treatments were as decided by the local team. Randomization was centralised with stratification by site and minimisation for age, sex, primary cancer stage, interval since primary resection, prior liver involvement, the number of metastases, and carcinoembryonic antigen. The assigned arm was not disclosed to the trial management group until completion of analysis. Analysis was on intention to treat with a margin for non-inferiority of 10%.

Findings: Between December 2010 and December 2016, 65 participants were randomised. Characteristics were well-matched in the two arms and similar to those in reported studies: age 35 to 86 (IQR 60 to 74); primary resection IQR 16 to 35 months previously; stage at resection T1, 2 or 3 in 3, 8 and 46; N1 or N2 in 31 and 26; unknown in 8. Lung metastases 1 to 5 (median 2); 16/65 had previous liver metastases; carcinoembryonic antigen normal in 55/65. There were no other interventions in the first 6 months, no cross overs from control to treatment, and no treatment related deaths or major adverse events. Hazard ratio for death within 5 years, comparing metastasectomy with control, was 0.82 (95%CI 0.43, 1.56).

Interpretation: The small number (N=65) precludes a conclusive answer to the research question but the similar survival in metastasectomy and control arms challenges beliefs that there is a 35% survival difference that can be attributed to lung metastasectomy.

Funding: Cancer Research UK funding Grant No. C7678/A11393
Name of the registry: Clintrial.gov
Registration number: NCT01106261
Date 19th April 2010
https://clinicaltrials.gov/ct2/show/NCT01106261
Usefulness of intra-operative neuromuscular blockade monitoring and reversal agents for postoperative residual neuromuscular blockade: a retrospective observational study
Authors: Domenech Gonzalo , Kampel Matias , Guzzo Maria Eugenia Garcia , Novas Delfina Sanchez , Terrasa Sergio , Fornari Gustavo Garcia 
Published: July 2019

Abstract
Background: Current neuromuscular blockade (NMB) management techniques cannot completely prevent residual NMB (RNMB) during the postoperative period. Evidently, compliance with NMB monitoring is persistently low, and the risk of RNMB during the perioperative period remains underestimated. To our knowledge, no publications have reported the incidence of RNMB in a university hospital where access to quantitative NMB monitoring and sugammadex is unlimited and where NMB management is not protocolised. The primary aim of this study was to estimate the incidence of RNMB in patients managed with or without sugammadex or neostigmine as antagonists and quantitative NMB monitoring in the operating room. The secondary aim was to explore the associations between RNMB and potentially related variables.
Methods: This retrospective observational cohort study was conducted at a tertiary referral university hospital in Buenos Aires, Argentina. Records created between June 2015 and December 2015 were reviewed. In total, 240 consecutive patients who had undergone elective surgical procedures requiring NMB were included. All patients were monitored via acceleromyography at the adductor pollicis muscle within 5 min of arrival in the postanaesthesia care unit (PACU). Scheduled recovery in the intensive care unit was the only exclusion criterion.
Results: RNMB was present in 1.6% patients who received intra-operative quantitative NMB monitoring and 32% patients whose NMB was not monitored (P < 0.01). Multivariable analysis revealed that the use of intra-operative quantitative NMB monitoring and sugammadex were associated with a lower incidence of RNMB, with calculated odds ratios of 0.04 (95% confidence interval [CI]: 0.005 to 0.401) and 0.18 (95% CI: 0.046 to 0.727), respectively.
Conclusions: The results of the present study suggest that intra-operative quantitative NMB monitoring and use of sugammadex are associated with a decreased incidence of RNMB in the PACU, reinforcing the contention that the optimal strategy for RNMB avoidance is the use of quantitative NMB monitoring and eventual use of reversal agents, if needed, prior to emergence from anaesthesia.
Effect of CYP3A5*3 polymorphism on anti-inflamatory therapy in children with bronchial asthma
Authors: Zastrozhina A. K. , Zaitseva S. V. , Grishina E. A. , Ryzhykova K. A. , Panfeorova O. O. , Kalenov S. Y. , Soboleva O. I. , Sychyov D. A. , Zakharova I. N. 
Published: July 2019

Previous pharmacogenetic studies demonstrate significant genes’ polymorphisms effect on the efficacy and safety of pharmacotherapy, including in bronchial asthma (BA). According to the literature, there are data on the effect of polymorphisms CYP3A4*22 and CYP3A5*3 on the efficacy of inhaled corticosteroids in children with BA. Further research on the effect of pharmacogenetic features on the efficacy and safety of drugs is one of the way to optimize asthma therapy in children.Purpose. Identification of possible ways to optimize asthma therapy by the analysis of CYP3A5 (A6986G) gene polymorphism effect on the asthma therapy efficacy.Materials and methods. The study was conducted on the basis of three children’s polyclinics of Moscow. 100 children aged 6–17 years with an established diagnosis of BA were included. Dynamic assessment of asthma control and the amount of therapy needed was carried out. All patients underwent genotyping for the A6986G polymorphism of CYP3A5 gene by real-time PCR. Statistical data analysis was carried out using a programming language for statistical data processing R.3.4.0.Results. It was found that 8% of children with asthma were heterozygous for the A6986G polymorphism of the CYP3A5 gene, 92% of respondents were homozygous with the GG genotype. In 6 out of 8 heterozygotes, the amount of control therapy corresponded the third and fourth therapy stages according to GINA criteria. In the group of moderate and severe BA, the number of heterozygotes for the A6986G polymorphic marker of the CYP3A5 gene was statistically higher compared to the group of children with mild BA (p = 0,048).Conclusion. Thus, we identified a statistically significant difference in the occurrence of heterozygotes for the A6986G polymorphism of the CYP3A5 gene between groups of children with mild asthma and patients with moderate and severe asthma. The AG genotype and the presence of the A allele (CYP3A5 gene (A6986G)) are associated with more severe BA and the need for more anti-inflammatory therapy.
The Contribution of the Infill Walls to the Lateral Strength of Concrete Frames
Authors: Christou Petros , Venizelou Christos 
Published: July 2019

Background:
Recent research studies conclude that the contribution of the infill walls to the overall lateral strength of frames is significant. The current state of the art includes two main approaches for the idealization of the behavior of the infill walls and their implementation in software. Micro modelling includes the use of the finite element method whereas the macro modelling, includes the use of one-dimensional compressive diagonal strut elements to replace the infill wall and provide the equivalent lateral stiffness.

Objective:
The aim of this study was to compare various methods for the simulation of the infill walls with the finite element method and propose an alternative approach which makes use of the rigid end offset which is a feature available in most of the finite element software.

Methods:
A reinforced concrete frame model with an infill wall was created. The model was modified to form combinations of infill wall thicknesses and values of Young’s modulus. The models were analyzed using the finite element method. The results were utilized to develop equations for the calculation of the length of rigid end offsets for the beams and columns of the frame. The rigid end offsets were then used in the analysis to numerically stiffen the frame and simulate an effective lateral strength contribution from the infill wall.

Results:
The results of the implementation of the rigid end offsets to simulate the contribution of the infill walls to the lateral stiffness of the frame were compared to the results of the results from the finite element analysis. Specifically, the results for the walls normally found in construction (less or equal to 3m in height and with thickness less or equal to 25cm) showed a very good agreement while the remaining results were very close.

Conclusion:
This work proposes equations for calculation of the length for the rigid end offsets which can be used in the analysis of frames with infill walls. The results show that the utilization of this feature from the structural analysis software in the analysis of frames, results in adequate stiffening of the overall frame, thus, providing an equivalent stiffness which accounts for the presence of the infill walls.