bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of BMPR2 ubiquitination-dependent degradation suppresses bladder cancer cell growth via downregulating Smurf1 expression with 8003-1841
Authors: Su Qiongli , Zhang Yuqing , Ling Wu , Tan Qiuhong , Wei Long , Xiao Di , Huang Li 
Published: October 2024

Abstract

Background
There is still a lack of effective treatment options for advanced bladder cancer (BC) in clinical practice. Smurf1 is an E3 ubiquitin ligase that enhances cancer cell growth and drug resistance through facilitating the ubiquitination and degradation of tumor suppressor proteins. However, there are no Smurf1 inhibitors approved for clinical use in cancer treatment at present. Similarly, there are currently no studies to confirm whether Smurf1 can be an effective therapeutic target for BC.
Methods
The biological effects of Smurf1 in BC were evaluated using MTT assay and colony formation assay. The mechanism by which Smurf1 promotes BC cell proliferation was investigated using Western blotting and Immunofluorescence. The Inhibitor of Smurf1 were screened using molecular docking. The changes of bladder cancer cells growth in vivo after Smurf1 inhibitors treatment were observed using a tumor xenograft model. The safety and mechanism of action of Smurf1 inhibitors in vivo were assessed using immunohistochemistry (IHC).
Results
In this study, our results revealed Smurf1 is high expressed in BC tissues, and the correlation between Smurf1 expression and poor prognosis in BC patients was evident. A set of in vitro and in vivo assays confirmed the role of Smurf1 in enhancing the proliferation of BC cells. Mechanistic studies revealed that Smurf1 promotes tumor progression by binding to BMPR2, facilitating BMPR2 ubiquitination and degradation in proteasome. Therefore, we further screened for effective inhibitors of Smurf1 from a ChemDiv database of 1,535,478 compounds in an attempt to explore new targeted drugs for BC. The results identified 8003 − 1841 as the strongest binder to Smurf1, which inhibited BC cell proliferation in vitro and in vivo by stabilizing the expression of BMPR2 through downregulation of Smurf1.
Conclusion
We have discovered a specific inhibitor, 8003 − 1841, that targets Smurf1 and effectively downregulates its expression. This inhibition of Smurf1 leads to the suppression of BC cell proliferation both in in vitro and in in vivo. Thus, our findings suggest that targeting Smurf1 could be a promising therapeutic approach for BC treatment. The inhibitor 8003 − 1841 shows potential as a treatment option for BC patients.
Novel Digital Markers of Sleep Dynamics: A CausalInference Approach Revealing Age and GenderPhenotypes in Obstructive Sleep Apnea
Authors: Bechny Michal , Kishi Akifumi , Fiorillo Luigi , Meer Julia van der , Schmidt Markus , Bassetti Claudio , Tzovara Athina , Faraci Francesca 
Published: October 2024

Abstract

Most individuals with sleep-disorders remain undiagnosed due to unawareness of symptoms or the high cost of polysomnographic (PSG) studies, impacting quality of life. Despite evidence that sleep-disorders alter sleep-stage-dynamics, clinical practice resists including these parameters in PSG-reports. We introduce a novel digital sleep-fingerprint, leveraging the matrix of sleep-stage-transition-proportions, enabling the derivation of several novel digital-markers and investigation of dynamics mechanisms. Using causal inference we address confounding in an observational clinical database and estimate personalized markers across ages, genders, and Obstructive-Sleep-Apnea (OSA) severities. Notably, our approach adjusts for five categories of sleep-wake-related-comorbidities, an aspect ignored in existing research, impacting 48.6% of OSA-subjects in our data. Key markers proposed, including NREM-REM-oscillations and sleep-stage-specific-fragmentations, were significantly increased across all OSA-severities and demographics. We also identified several OSA-gender-phenotypes, suggesting higher vulnerability of females to awakening and REM-sleep disruptions. Considering advances in automated-sleep-scoring and wearables, our approach can enable novel, low-cost screening tools.
Sociocultural Determinants of Antimicrobial Resistance in the Islamic Republic of Iran: A Qualitative Study
Authors: Mehtarpour Mojtaba , najafi zahra , Jaafaripooyan Ebrahim 
Published: October 2024

Abstract

Introduction:
Addressing antimicrobial resistance (AMR) requires consideration of the One Health approach and the sociocultural context unique to each country. This study aimed to examine the sociocultural factors influencing the development of AMR in Iran.
Methods
This qualitative study employed in-depth, face-to-face interviews. A total of 57 policymakers, managers, and service providers from both human and animal health sectors were interviewed using purposive and snowball sampling methods. The data were analyzed using framework analysis.
Findings
The study identified several factors contributing to the development of AMR, including demographic changes, improper drug use by the public, cultural challenges for service providers, lack of awareness and knowledge among livestock farmers, and general public attitudes toward drug use.
Conclusion
Sociocultural factors in both the human and animal health sectors significantly influence the use of antimicrobial drugs and the escalation of AMR across different ecosystems. The wide range of sociocultural influences among various stakeholders—such as service providers (physicians and veterinarians), the general public, and livestock farmers—highlights the necessity for policymakers to consider these groups when planning interventions to control AMR.
Data mining and function analysis of a key ferroptosis target FANCD2 positively correlated with PD-L1 expression
Authors: Chen Han , Huang Xueyuan , Li Lin , Nian Siji , Ye Yingchun , Yuan Qing , Huang Zhanwen , Xu Xiaomei , Liao Jue 
Published: October 2024

Abstract

Iron is required for the control of ferroptosis, a pattern of programmed cell death brought on by the buildup of reactive oxygen species and lipid peroxidation. Inducing ferroptosis may have great therapeutic promise for tumor cells, according to an increasing number of studies. However, it is unclear how ferroptosis works to treat lung cancer. For prognostic analysis of expression of 25 ferroptosis-related genes, consensus clustering analysis, LASSO model development, and association analysis with PD-L1, we gathered clinical and molecular data of LUAD patients from the TCGA database and the GEO database. We discovered that the ferroptosis gene FANCD2 had the best prognostic value of any gene based on the findings. Then, using multicolor immunofluorescence to identify the mutual regulatory link between FANCD2 and PD-L1 in LUAD tissues, we discovered that both of these genes were highly expressed and co-expressed in LUAD tissues. Finally, using four study axes—mutation, tumor microenvironment, immune infiltration, and pan-cancer—we discovered that FANCD2 may have a direct or indirect role in the aforementioned processes, influencing how well LUAD patients would fare. In conclusion, this research offers proof in favor of finding novel, potential immune targets for treatment as well as refining PD-L1 antibody immunotherapy for LUAD. In-depth analysis of the FANCD2 gene and the creation of associated medications may enhance prognosis and increase median survival time in LUAD patients.
Home-based continuous passive motion versus conventional physical therapy: a randomized follow-up trial after release of degenerative elbow stiffness
Authors: Li Haibin , Wang Ming , Deng Guangming , Fu Hongrui , Chen Bingbing , Yang Xueqiao 
Published: October 2024

Abstract

Background/Objective:Continuous Passive Motion (CPM) has been used in rehabilitation for several decades. However, there is a lack of randomized controlled trials (RCTs) comparing CPM with conventional physical therapy (PT) for rehabilitation after the release of elbow contracture. Although some studies suggest that CPM may improve postoperative range of motion, its effectiveness compared to PT in functional recovery remains unclear. This study aims to evaluate the effectiveness of home-based immersive CPM training for rehabilitation following elbow contracture release, compared to manual physical therapy, and to determine its role in promoting functional recovery of the elbow.
Methods:This single-blind, single-center RCT retrospectively analyzed 40 patients with moderate to severe elbow osteoarthritis who underwent standardized arthroscopic release around September 1, 2023. The patients were divided into two groups: 20 received home-based immersive CPM rehabilitation, while 20 underwent conventional PT, with comparisons made across baseline characteristics, surgical techniques, and rehabilitation protocols.
Results:The average follow-up time was 3 months. The CPM group showed greater improvements in ROM, increasing by 83.03% (from 69.25$^\circ$ pre-op to 126.75$^\circ$), compared to a 61.29% increase in the PT group (from 69.75$^\circ$ to 112.50$^\circ$). MEPS scores in the CPM group rose by 30.41% (from 74.00 to 96.50), while the PT group saw a 24.83% improvement (from 72.50 to 90.50). Additionally, the CPM group had significantly lower rehabilitation costs (500 RMB/month vs. 6000-9000 RMB for PT).
Conclusion: Home-based immersive CPM rehabilitation proved more effective and cost-efficient than conventional PT, showing greater improvements in ROM, superior pain management, and better functional recovery following elbow surgery.
WITHDRAWN: Postoperative Joint Scores of Comparison between total and unicondylar knee arthroplasty：A matched comparison
Authors: Liu Chen-ci , Zhang Zhen-dong , Shi Chen-hui 
Published: October 2024

Abstract

Objective：To investigate the patient self-reported outcomes and clinical results of unicondylar arthroplasty versus total knee arthroplasty for osteoarthritis of the knee. To evaluate the early functional recovery of patients after both surgical modalities.
Methods：In this retrospective study, 52 patients (26 patients in the unilateral UKA group; 26 patients in the unilateral TKA group) with osteoarthritis of the knee were treated with unicondylar arthroplasty and total knee arthroplasty to compare the baseline and perioperative data of the patients in the UKA group and the TKA group, the modified Postoperative Joint Forgetting Score (MFJS), Oxford Early Recovery Score for Knee Arthroplasty (OARS) at 1, 2, 6 week and 3 month postoperatively, and Oxford Early Change Score for Knee Arthroplasty (OACS) at 1, 2, 6 week, and 3, 6 and 12 month postoperatively.
Results：The general clinical data (age, gender, body mass index) of patients in the UKA and TKA groups were not statistically significant (P>0.05) and were comparable.The operation time and intraoperative bleeding in the UKA group were lower than those in the TKA group (P<0.001). At 3, 6 and 12 month after surgery, the MFJS scores of patients in the UKA group were higher than those of patients in the TKA group (P<0.001). OARS scores at 1, 2, 6 week and 3 month postoperatively were higher in the UKA group than in the TKA group (P<0.05). There was no statistically significant difference in the comparison of OACS scores between the unilateral UKA and TKA groups at 1 week postoperatively (P>0.05), and the scores of the unilateral UKA group were higher than those of the unilateral TKA group at 2, 6 week, and 3, 6, and 12 month (P<0.05).
Conclusion：Both UKA and TKA are effective in improving functional scores in end-stage osteoarthritis of the knee.UKA has better knee proprioception and faster postoperative recovery compared to TKA.
ANX-Net: A Fast and Resource Optimized Network for Image Dehazing for Driving in Haze Weather Conditions
Authors: Zhu Yun , Niu Shaoshan , Jia Guo , Su Yan 
Published: October 2024

Abstract

Severe weather conditions such as haze will bring serious problems to the safe driving of autonomous vehicle. In order to ensure that autonomous vehicle can still run safely in frequent bad weather, the research of image dehazing algorithm is very important. The key to safe and reliable driving is that autonomous vehicle can obtain clear images in severe haze weather conditions. Therefore, ensuring the dehazing performance of the dehazing algorithm is very important. In this paper, we propose ANX-Net, which is a robust and reliable dehazing network for autonomous vehicle. The network uses components such as feature extraction module, channel attention module, multi-scale spatial attention module and gsconv module to effectively dehaze the images taken by the autonomous vehicle camera. Through a detailed qualitative and quantitative evaluation of the road traffic dataset AAR in hazy weather, the effectiveness of the proposed network was analyzed, demonstrating its good dehazing performance.
The vascular anatomy and harvesting of the medial femoral condyle flap in pigs
Authors: Zuo Yanhai , Yi Lei 
Published: October 2024

Abstract

Introduction Clinically, there has been increasing employment of the medial femoral condyle flap. Pigs have been employed as a model of the medial femoral condyle flap. However, related anatomical studies from the perspective of comparative medicine are rare. The objective of this study was to explore the vascular anatomy of the medial femoral condyle in pigs, explore the surgical procedure of the medial femoral condyle flap in pigs, and compare the medial femoral condyle flap in pigs and humans.Materials and Methods 20 fresh cadaveric hindlimbs of hybrid pigs were used in this study. The origination, course, and branches of the vessels nourishing the medial femoral condyle were observed in 15 specimens. The variability in the anatomy of the vessels and the length and outer diameter of the vessels were evaluated. Surgical procedures for the medial femoral condyle flap were conducted on five specimens.Results The nourishing artery of the medial femoral condyle in pigs was the second descending geniculate artery, which was observed in all 15 pigs and had a diameter and length of 2.013 ± 0.316 mm and 1.527 ± 0.212 cm, respectively. A skin incision of approximately 10 cm was used to harvest the medial femoral condyle flap of the pig in the supine position. After the adductor muscle was cut, the whole course of the second descending geniculate artery artery and its venae comitantes were exposed.Conclusions The vascular anatomy of the medial femoral condyle in pigs and that of humans exhibited great similarities. The medial femoral condyle flap in pigs was as easily harvested as that in humans. Pigs could serve as a suitable animal model for the medial femoral condyle flap.
Comparison of Central and Paracentral Targeting in Ultrasound-Guided Stellate Ganglion Block: A Randomized Controlled Study
Authors: Peng Zhiyou , Zhang Yanfeng , Han Lulu 
Published: October 2024

Abstract

Objective
Ultrasound-guided stellate ganglion block (SGB) plays a crucial role in managing chronic pain syndromes. This study aims to compare the procedure times and therapeutic efficacy of ultrasound-guided SGB when the targeting is centered versus paracentral on the ultrasound screen.
Methods
A total of 80 patients in need of SGB were randomly divided into two groups. In the central targeting group, the target was placed precisely at the center of the ultrasound screen. In the paracentral targeting group, the target was positioned slightly off-center. The time taken to complete the procedure, the Horner Syndrome and adverse reactions were recorded and analyzed.
Results
The paracentral group showed shorter SGB operation duration than the central group(P < 0.05). Horner Syndrome occurred in all patients of two groups after SGB. One case of hoarseness and 1 cases of slowed pulse occurred in the paracentral group, while 2 case of hoarseness and 1 cases of neural stimulation paresthesia occurred in the central group (P > 0.05).
Conclusion
The study results indicate that paracentral targeting in ultrasound-guided SGB may offer shorter procedure times and compared to central targeting. Nevertheless, both methods demonstrated comparable therapeutic efficacy. These findings can guide clinicians in optimizing SGB techniques to improve patient outcomes.
Shock Index Identifies Compensated Shock in the ‘Normotensive’ Trauma Patient
Authors: Lin Theodore M , Memon Ali M. , Reeson Emily A. , Tolan Grace C. , Low Trevor M. , Kupanoff Kristina M. , Huang Dih-Dih , Jones Michael D. , Czarkowski Brian R. , Soe-Lin Hahn , Bogert James N. , Weinberg Jordan A. 
Published: October 2024

Abstract

Introduction:
Hemorrhagic shock is a life-threatening condition that requires rapid identification for timely intervention. Although shock is easily discernible in the hypotensive patient, compensated shock in the "normotensive" patient is not. This study aimed to evaluate the utility of shock index (SI) in trauma patients with compensated shock.
Methods
Patients with SBP > 90 mmHg on arrival were identified from our trauma center registry. SI was calculated by arrival heart rate divided by arrival SBP. Patients were stratified by SI using the following thresholds: < 0.7, > 0.7 to 0.9, > 0.9 to 1.1, > 1.1 to 1.3, and > 1.3. Logistic regression was used to estimate the odds of transfusion within 1 hour of arrival for each SI category with < 0.7 as the referent.
Results
5964 trauma patients were included. Blood products were transfused within 1 hour of arrival in 221 (3.7%) patients. A main effect was observed for shock index with increased risk for required transfusion for patients with admission shock index > 0.7 (P < 0.001). In comparison to shock index of < 0.7, odds ratios were 2.4(1.6–3.6), 8.3(5.5–12.3), 24.7(15.1–40.5), 63.3(35.0–114.4) for each categorical increase in SI.
Discussion
SI can identify trauma patients with compensated shock; among patients presenting without hypotension, arrival SI was associated with an increase in odds of receiving transfusion within one hour. Arrival SI should be considered as a criterion for activation of massive transfusion protocol and highest level of trauma activation for non-hypotensive patients.
СТЕПНОЙ ЛАНДШАФТ СЕВЕРНОГО ПРИАЗОВЬЯ КАК ФАКТОР В СУДЬБАХ НАСЕЛЕНИЯ
Authors: Л.Г. ШЕПКО 
Published: October 2024

Ретроспективный взгляд на исторические этапы южнорусских степей позволяет увидеть взаимодействие природно-географических условий и социальных факторов в процессе развития цивилизаций. Длительное время Северное Приазовье было территорией, где достаточно успешно осуществлялись стратегии кочевников, набеги и нашествия, совершаемые в отношении соседних народов и государств. В эпоху хазарского каганата население испытывает воздействия нескольких цивилизаций (распространении монотеистических религий, участие государства в организации пространственной структуры, формирование однородной материальной культуры). В рамках Российской империи шло целенаправленное упорядочивание приазовских территорий. Территориальное расширение пределов империи закономерно влекло за собой и усложнение административно-территориальной структуры и развитие урбанистических элементов.
A retrospective look at the historical stages of the southern Russian steppes allows for witnessing the interaction of natural and geographical conditions and social factors in the process of civilization development. Over centuries the Northern Azov region was a territory where nomadic strategies, raids and invasions against neighboring peoples and states were being implemented quite successfully. During the era of the Khazar Khaganate, the population experienced the influence of several civilizations, the spread of monotheistic religions, the participation of the state in the organization of the spatial structure, and the formation of a homogeneous material culture. Within the framework of the Russian Empire, a targeted organization of the Azov territories occurred. The territorial expansion of the empire's borders naturally entailed the complication of the administrative-territorial structure and the development of urban elements.
ВЛИЯНИЕ КЛИМАТА НА ВОЕННУЮ АКТИВНОСТЬ НОМАДОВ ВОСТОЧНОЙ ЕВРОПЫ В РАННЕМ ЖЕЛЕЗНОМ ВЕКЕ
Authors: Е.В. ВДОВЧЕНКОВ 
Published: October 2024

В числе факторов, определяющих жизнь кочевников, важнейшими были природно-климатические условия. В главе анализируется влияние климата на военно-политическую активность кочевников Восточной Европы раннего железного века. В настоящее время реконструкция климата наиболее успешно реализуется почвоведами при анализе погребенных почв в курганных могильниках. Подтверждена зависимость активности номадов от природно-климатических условий. На период гумидизации приходится пик активности кочевых объединений. В период аридизации, при неблагоприятных условиях, уменьшается активность номадов, ослабляются их объединения, проявляется тенденция к седентаризации кочевников. Ключевые слова: ранний железный век, степной ландшафт, Восточная Европа, климат, аридизация, гумидизация, скифы, сарматы, экзополитарный способ производства.
Among the factors determining the life of nomads,the most important were the naturaland climatic conditions. The article analyzes the influence of climate onthe military and political activityof the nomads of Eastern Europeof the Early Iron Age. Currently, climate reconstructionis most successfully implemented by soil scientists when analyzing buried soils in burial mounds. The dependenceof nomad activity on natural and climatic conditionshas been confirmed. During the period of humanization, the peak activityof nomadic associations occurs. During the period of aridization, under unfavorable conditions, the activity of nomads decreases, their associations weaken, and the tendency to sedentarization of nomads manifestsitself. Keywords: early Iron Age, steppe landscape, Eastern Europe, climate, aridization, humanization, Scythians, Sarmatians, exopolitan mode of production.
Anais da 49ª Semana Científica Odontológica - Faculdade de Odontologia, Universidade Federal de Alfenas - UNIFAL - MG, Edição 2024
Authors: Reis Leonardo Amaral dos , Freitas Amanda Beatriz Dahdah Aniceto de , Haddad Danziger Marcela Filié , Almeida Daniel Augusto de Faria , Fernandes Leandro Araújo 
Published: October 2024

Caro(a) Leitor(a)
Nos dias 2, 3 e 4 de outubro de 2024, a Faculdade de Odontologia da Universidade Federal de Alfenas sediou a 49ª Semana Científica Odontológica da UNIFAL. Este evento teve como objetivo promover atividades na área de Odontologia, conduzidas por professores renomados, que trouxeram atualizações valiosas para alunos e profissionais, tanto na prática clínica quanto na pesquisa científica. O evento contou com a participação de mais de 300 inscritos e ofereceu palestras e minicursos que enriqueceram ainda mais a experiência dos participantes. Contamos com cerca de 140 trabalhos apresentados, distribuídos nos três pilares da aprendizagem: pesquisa, ensino e extensão. Os estudantes do curso de Odontologia tiveram a oportunidade de apresentar seus trabalhos em formato de painel e apresentação oral. Os membros da banca examinadora estabeleceram notas para os trabalhos, e ao final, 12 deles receberam menções honrosas, divulgadas no encerramento do evento. Além disso, recebemos a participação de alunos de graduação e pós-graduação de instituições externas, assim como de profissionais da odontologia da região de Alfenas. Aproveito a oportunidade para agradecer ao periódico Archives of Health Investigation, na pessoa da Profa. Associada Maria Cristina Rosifini Alves Rezende, pela honrosa oportunidade de publicar os resumos dos trabalhos apresentados no evento. Meus sinceros agradecimentos. Atenciosamente,
Prof. Dr. Leonardo Amaral dos Reis
Presidente - 49ª Semana científica Odontológica da UNIFAL, Edição 2024