bluuebunny/crossref_metadata_2025_split huggingface dataset:
PDLIM1 Inhibits Tumor Metastasis Through Activating Hippo Signaling in Hepatocellular Carcinoma
Authors: Huang Zhao , Zhou Jian‐Kang , Wang Kui , Chen Haining , Qin Siyuan , Liu Jiayang , Luo Maochao , Chen Yan , Jiang Jingwen , Zhou Li , Zhu Lei , He Juan , Li Jiao , Pu Wenchen , Gong Yanqiu , Li Jianbo , Ye Qin , Dong Dandan , Hu Hongbo , Zhou Zongguang , Dai Lunzhi , Huang Canhua , Wei Xiawei , Peng Yong 
Published: September 2019

Background and Aims
Tumor metastasis is a major factor of high recurrence and mortality in hepatocellular carcinoma (HCC), but its underlying mechanism remains elusive. We report that PDZ and LIM domain protein 1 (PDLIM1) is significantly down‐regulated in metastatic human HCC tissues, which predicts unfavorable prognosis, suggesting that PDLIM1 may play an important inhibitory role during HCC metastasis.

Approach and Results
Functional studies indicate that PDLIM1 knockdown induces epithelial‐to‐mesenchymal transition (EMT) of HCC cells, elevates their invasive capacity, and promotes metastasis in vitro and in vivo, whereas overexpression of PDLIM1 exhibits opposite phenotypes. Mechanistically, PDLIM1 competitively binds to the cytoskeleton cross‐linking protein alpha‐actinin 4 (ACTN4), leading to the disassociation of ACTN4 from F‐actin, thus preventing F‐actin overgrowth. In contrast, loss of PDLIM1 induces excessive F‐actin formation, resulting in dephosphorylation of large tumor suppressor kinase 1 and activation of Yes‐associated protein, thereby promoting HCC metastasis. Moreover, Asn145 (N145) of PDLIM1 is critical for its interaction with ACTN4, and N145A mutation abolishes its regulatory function in Hippo signaling and HCC metastasis.

Conclusions
Our findings indicate that PDLIM1 suppresses HCC metastasis by modulating Hippo signaling, suggesting that PDLIM1 may be a potential prognostic marker for metastatic HCC.
Development of atypical parental behavior during an inpatient family preservation intervention program
Authors: Vischer Anne‐Fleur W. K. , Post Wendy J. , Grietens Hans , Knorth Erik J. , Bronfman Elisa 
Published: September 2019

ABSTRACTSince failed reunification is a detrimental outcome for children, particularly infants and toddlers, the aim of this study was to gain insight into support to families in multiple‐problem situations to help them achieve sustainablegood‐enoughparenting. Therefore, we examined outcomes of an assessment‐based inpatient family preservation program. We prepared a thorough target‐population description (n= 70) using file analysis. Next, we examined atypical parental behavior during the intervention using the Atypical Maternal Behavior Instrument for Assessment and Classification with a repeated measures design (n= 30). The family files revealed a great number of issues at the family, parent, and child levels, such as practical matters, problems in parent functioning and between parents, and difficulties in the broader environment. We found a significant decline in three dimensions of atypical parental behavior over time. This program has great potential in supporting vulnerable families in their pursuit of family preservation.
Exercise Degrades Bone in Caloric Restriction, Despite Suppression of Marrow Adipose Tissue (MAT)
Authors: McGrath Cody , Sankaran Jeyantt S , Misaghian-Xanthos Negin , Sen Buer , Xie Zhihui , Styner Martin A , Zong Xiaopeng , Rubin Janet , Styner Maya 
Published: September 2019

ABSTRACT
Marrow adipose tissue (MAT) and its relevance to skeletal health during caloric restriction (CR) is unknown: It remains unclear whether exercise, which is anabolic to bone in a calorie-replete state, alters bone or MAT in CR. We hypothesized that response of bone and MAT to exercise in CR differs from the calorie-replete state. Ten-week-old female B6 mice fed a regular diet (RD) or 30% CR diet were allocated to sedentary (RD, CR, n = 10/group) or running exercise (RD-E, CR-E, n = 7/group). After 6 weeks, CR mice weighed 20% less than RD, p &lt; 0.001; exercise did not affect weight. Femoral bone volume (BV) via 3D MRI was 20% lower in CR versus RD (p &lt; 0.0001). CR was associated with decreased bone by μCT: Tb.Th was 16% less in CR versus RD, p &lt; 0.003, Ct.Th was 5% less, p &lt; 0.07. In CR-E, Tb.Th was 40% less than RD-E, p &lt; 0.0001. Exercise increased Tb.Th in RD (+23% RD-E versus RD, p &lt; 0.003) but failed to do so in CR. Cortical porosity increased after exercise in CR (+28%, p = 0.04), suggesting exercise during CR is deleterious to bone. In terms of bone fat, metaphyseal MAT/ BV rose 159% in CR versus RD, p = 0.003 via 3D MRI. Exercise decreased MAT/BV by 52% in RD, p &lt; 0.05, and also suppressed MAT in CR (−121%, p = 0.047). Histomorphometric analysis of adipocyte area correlated with MAT by MRI (R2 = 0.6233, p &lt; 0.0001). With respect to bone, TRAP and Sost mRNA were reduced in CR. Intriguingly, the repressed Sost in CR rose with exercise and may underlie the failure of CR-bone quantity to increase in response to exercise. Notably, CD36, a marker of fatty acid uptake, rose 4088% in CR (p &lt; 0.01 versus RD), suggesting that basal increases in MAT during calorie restriction serve to supply local energy needs and are depleted during exercise with a negative impact on bone. © 2019 The Authors. Journal of Bone and Mineral Research published by American Society for Bone and Mineral Research.
Detection of copy number disorders associated with congenital anomalies of the kidney and urinary tract in fetuses via single nucleotide polymorphism arrays
Authors: Cai Meiying , Lin Na , Su Linjuan , Wu Xiaoqing , Xie Xiaorui , Li Ying , Chen Xuemei , Dai Yifang , Lin Yuan , Huang Hailong , Xu Liangpu 
Published: September 2019

AbstractBackgroundWhile congenital anomalies of the kidney and urinary tract (CAKUT) constitute one‐third of all congenital malformations, the mechanisms underlying their development are poorly understood. Some studies have reported an association between CAKUT and copy number variations (CNVs) in children and adults, but few have focused on chromosomal microarray analysis (CMA) findings in fetuses with CAKUT. Therefore, we aimed to perform a CMA on fetuses with CAKUT and normal karyotypes in the presence and absence of other structural anomalies.MethodThe study was conducted in 147 fetuses with CAKUT and normal karyotypes between January 2016 and January 2019 in the Fujian Provincial Maternal and Child Health Hospital. Single nucleotide polymorphism (SNP) analysis was performed using the Affymetrix CytoScan HD platform.ResultsThe SNP array identified abnormal CNVs in 13 cases (8.8%): Six were pathogenic, and seven were variations of uncertain clinical significance (VOUS). The detection rate of abnormal CNVs in non‐isolated CAKUT was higher than that in isolated CAKUT (22.7% vs 6.4%, P = .038). Within the abnormal CNV groups, the highest frequency of CNVs was identified in fetuses with polycystic kidney dysplasia (13.5%), followed by those with renal agenesis (10.5%).ConclusionSNP array is effective for identifying chromosomal abnormalities in CNVs in fetuses with CAKUT and normal karyotypes, and help counseling.
The loss of innocence: Confronting class differences in the practice of psychotherapy
Authors: Bodnar Susan 
Published: September 2019

AbstractA young woman sought psychotherapy for complaints that initially framed her as quite troubled, presenting as an individual with a borderline personality disorder. Over the course of therapy, it became clear that she more accurately suffered from a form of status anxiety. In working with people from different class backgrounds, it is very important for the clinician to differentiate psychological problems that arise from the stressors associated with class status from those that emerge from more internal disturbance.
Understanding the role of service providers, land use, and resident characteristics on the occurrence of mental health crisis calls to the police
Authors: White Clair , Goldberg Victoria , Hibdon Julie , Weisburd David 
Published: September 2019

AbstractSimilar to concentrations of crime, mental health calls have been found to concentrate at a small number of places, but few have considered the context of places where mental health calls occur. The current study examines the influence of the physical and social context of street segments, particularly the role of service providers, land use features of the street and nearby area, and characteristics of residents on the likelihood of a mental health crisis call to the police occurring on the street. The findings demonstrate that the social context, such as offending and drug use among residents, levels of social cohesion and community involvement, and drug and violent crime influenced the occurrence of mental health crisis calls. Findings from this study make theoretical and practical contributions to a number of disciplines by improving our understanding of where mental health crisis calls occur and why they are found at specific places.
MicroRNA‐181a exerts anti‐inflammatory effects via inhibition of the ERK pathway in mice with intervertebral disc degeneration
Authors: Sun Yanpeng , Shi Xiangqin , Peng Xiaodong , Li Yanzhou , Ma Husheng , Li Dongfang , Cao Xiangyang 
Published: September 2019

AbstractEnzymatic decomposition of extracellular matrix and possibly local inflammation may cause intervertebral disc degeneration (IDD). MicroRNAs have been reported to correlate with the development of IDD. In this experiment, we aim at finding out the role of miR‐181a in the inflammation of IDD and the underlying mechanism. The targeting relationship between miR‐181a and tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) was verified. Following the establishment of IDD mouse models, disc height index (DHI) and the change of DHI (%DHI) were measured. The functional role of miR‐181a in IDD was determined using ectopic expression and depletion and reporter assay experiments. Expression of miR‐181a, TRAIL, extracellular signal‐regulated kinase (ERK) pathway‐related genes and inflammatory factors was evaluated. Also, the expression of collagen I and collagen II was observed. miR‐181a directly targeted TRAIL. IDD mice exhibited significant degeneration of the intervertebral disc. miR‐181a was downregulated while TRAIL was upregulated in mice with IDD. miR‐181a upregulation and the ERK pathway inhibition could reduce expression of TRAIL, ERK pathway‐related genes, inflammatory factors, and collagen I, but promote collagen II expression. Our results reveal that upregulation of miR‐181a protects against inflammatory response by inactivating the ERK pathway via suppression of TRAIL in IDD mice. These results point to miR‐181a as a potential therapeutic target for the clinical management of IDD.
Differentially methylated gene patterns between age‐matched sarcopenic and non‐sarcopenic women
Authors: He Lingxiao , Khanal Praval , Morse Christopher I. , Williams Alun , Thomis Martine 
Published: September 2019

AbstractBackgroundSarcopenia is characterized by progressive decreases in muscle mass, muscle strength, and muscle function with ageing. Although many studies have investigated the mechanisms of sarcopenia, its connection with epigenetic factors, such as DNA methylation, still remains poorly understood. The aim of this study was to explore sarcopenia‐related DNA methylation differences in blood samples between age‐matched sarcopenic and non‐sarcopenic older women.MethodsA sarcopenic group (n = 24) was identified and selected from a set of 247 older Caucasian women (aged 65–80 years) based on cut‐off points of skeletal muscle index at 6.75 kg/m2 and grip strength at 26 kg (the lower quintile of grip strength in the set). A non‐sarcopenic group (n = 24) was created with a similar age distribution as that of the sarcopenic group. DNA methylation patterns of whole blood samples from both groups were analysed using Infinium MethylationEPIC BeadChip arrays. Differentially methylated cytosin–phosphate–guanine sites (dmCpGs) were identified at a P value threshold of 0.01 by comparing methylation levels between the sarcopenic and non‐sarcopenic groups at each CpG site. dmCpG‐related genes were annotated based on Homo sapiens hg19 genome build. The functions of these genes were further examined by GO and KEGG pathway enrichment analysis.ResultsThe global methylation level of all analysed CpG sites (n = 788 074) showed no significant difference between the sarcopenic and non‐sarcopenic groups (0.812), while the average methylation level of dmCpGs (n = 6258) was significantly lower in the sarcopenic group (0.004). The sarcopenic group had significantly higher methylation levels in TSS200 (the region from transcription start site to 200 nucleotides upstream of the site) and lower methylation levels in gene body and 3'UTR regions. In respect of CpG regions, CpG islands in promoters and some intragenic regions showed greater levels of methylation in the sarcopenic group. dmCpG‐related KEGG pathways were mainly associated with muscle function, actin cytoskeleton regulation, and energy metabolism. Seven genes (HSPB1, PBX4, CNKSR3, ORMDL3, MIR10A, ZNF619, and CRADD) were found with the same methylation direction as previous studies of blood sample methylation during ageing. Fifty‐four genes were shared with previous studies of resistance training.ConclusionsOur results improve understanding of epigenetic mechanisms of sarcopenia by identifying sarcopenia‐related DNA methylation differences in blood samples of older women. These methylation differences suggest underlying alterations of gene expression and pathway function, which can partially explain sarcopenia‐related muscular changes.
Mechanochemical synthesis and cycloaddition reactions of fluoro nitrone under solvent‐free conditions and potential antimicrobial activities of the cycloadducts
Authors: Chakraborty Bhaskar 
Published: September 2019

AbstractSynthesis and cycloaddition reactions of fluoro nitrone under solvent‐free conditions using ball‐milling technique have been reported. Significant change in rate and yields of the cycloadducts have been noticed compared with solvent‐free microwave‐induced reactions of fluoro nitrones. The present study reports synthesis of N‐benzyl fluoro nitrone and its cycloaddition reactions with maleimides and few electron deficient alkynes under solvent‐free conditions. The synthesized fluoro cycloadducts were found to exhibit potential antimicrobial activities.
RNF144B inhibits LPS-induced inflammatory responses via binding TBK1
Authors: Zhang Zhen , Zhang Luoyan , Wang Bin , Zhu Xiaoxiao , Zhao Lin , Chu Chu , Guo Qiang , Wei Ran , Yin Xunqiang , Zhang Yunhong , Li Xia 
Published: September 2019

Abstract
Innate immune responses need to be precisely controlled to avoid prolonged inflammation and prevent unwanted damage to the host. Here, we report that RNF144B responded dynamically to LPS stimulation and negatively regulated LPS-induced inflammation. We found that RNF144B interacted with the scaffold/dimerization domain (SDD) of TANK binding kinase 1 (TBK1) through the in between RING (IBR) domain to inhibit its phosphorylation and K63-linked polyubiquitination, which led to TBK1 inactivation, IRF3 dephosphorylation, and IFN-β reduction. RNF144B knockdown with siRNA increased IRF3 activation and IFN-β production in response to LPS stimulation. Our study identifies that RNF144B interaction with TBK1 is sufficient to inactivate TBK1 and delineates a previously unrecognized role for RNF144B in innate immune responses.
Intensified hydroclimatic regime in Korean basins under 1.5 and 2°C global warming
Authors: Kim Jeong‐Bae , Im Eun‐Soon , Bae Deg‐Hyo 
Published: September 2019

AbstractThis study assesses the potential changes in regional hydroclimates over South Korea in response to 1.5 and 2.0°C of global warming above preindustrial levels based on multimodel ensemble projections forced by a representative concentration pathway (RCP4.5) scenario. The meteorological inputs, which are derived from five global climate models after removing systematic bias using quantile mapping, are fed into a distributed hydrological model, the variable infiltration capacity model, to estimate the hydrologic responses to different levels of greenhouse gas concentrations in future periods. The changes in seasonal mean precipitation differ between monsoon and intermonsoon seasons. An increase in summer precipitation and a decrease in winter precipitation commonly occur under 1.5 and 2.0°C of global warming, resulting in intensified precipitation seasonality. However, changes in spring and fall precipitation show opposite change signals or relatively little robustness (as measured by model agreement) in response to different degrees of warming. Spatial and seasonal changes in precipitation are directly transferred to runoff patterns, increasing the disparity between wet and dry seasons. Global warming also leads to changes in the distributions of daily precipitation and streamflow, and the projected changes systematically involve an increase in high‐intensity precipitation and a decrease in relatively low‐intensity precipitation. This behaviour tends to be amplified under 2.0°C in comparison to 1.5°C of global warming, with potential implications for increased water stress under a much warmer climate. More importantly, under 2.0°C of global warming, the magnitude of extremes such as the annual maximum day flow in Korean basins is likely to be enhanced. This study demonstrates that changes in precipitation characteristics can explicitly modulate runoff and subsequently streamflow patterns, suggesting positive benefits of half a degree less warming in terms of the frequency and intensity of extreme streamflow.
Review of various measurement methodologies of migration ion influence on LCD image quality and new measurement proposal beyond LCD materials
Authors: Inoue Masaru 
Published: September 2019

AbstractNowadays, thin‐film transistor liquid crystal displays (TFT‐LCDs) have realized high reliability of display characteristics by improving liquid crystal (LC) materials and cell fabrication processes. In order to improve display reliability, measurement methodologies are important to see the progress of improvement of materials and processes; thus, our group has proposed voltage holding ratio (VHR), ion impurity, residual direct current (DC) and elastic constants for LC cells, and the optical anisotropy of an alignment layer on indium tin oxide (ITO) glass substrate for LCD industry. In case of an ion impurity, we have succeeded in measuring the ion impurity amount in TFT‐LCD. Furthermore, we have recently proposed ion impurity measurement methodology for beyond LCD applications that are organic light emitting diode (OLED) and organic photovoltaics (OPV).In this review, I introduce each measurement methodology for LCDs and beyond LCDs in detail.
Multicentric study applying the european laryngological society classification of benign laryngotracheal stenosis in adults treated by tracheal or cricotracheal resection and anastomosis
Authors: Fiz Ivana , Monnier Philippe , Koelmel Jan C. , Di Dio Diana , Fiz Francesco , Missale Francesco , Piazza Cesare , Peretti Giorgio , Sittel Christian 
Published: September 2019

Objectives/HypothesisIntroduction and widespread use of cricotracheal resection and anastomosis (CTRA) as routine treatment for high‐grade benign laryngotracheal stenosis (LTS) led to the need for a new classification system that could accurately predict surgical outcomes by integrating crucial stenosis and patient‐related information. In 2015, the European Laryngological Society (ELS) proposed a new classification for benign LTS. We retrospectively tested it in adults treated at three referral centers to assess its reliability in predicting surgical outcomes.Study DesignRetrospective cohort study.MethodsWe included 166 adults treated by open tracheal resection and anastomosis (TRA) and CTRA procedures, restaged according to the ELS classification evaluating grade of stenosis (I–IV, Myer‐Cotton), number of subsites involved, and presence of systemic comorbidities. We correlated these parameters with decannulation, number of retreatments, and complications.ResultsFinal decannulation was predicted by a proposed ELS score ≥ IIIb, history of previous treatment, and length of resection (P < .05). Decannulation was achieved in 99% of patients without and in 88% of patients with surgical complications (P < .01). The incidence of surgical complications was related to the proposed ELS score (P < .01); an ELS score < IIIb showed a lower complication rate compared to patients with a ≥ IIIb score (32.8% vs. 57.7%, P < .01). Additional treatment was required in 73 (44%) patients (mean = 2.7 ± 2.2, range = 1–11). ELS score ≥ IIIb, length of resection, and occurrence of surgical complications predicted the number of such treatments (P < .05, P < .05, and P < .001, respectively).ConclusionsELS classification of benign LTS is able to accurately predict success in adult TRA/CTRA procedures and may be helpful in choice of therapy and patient counseling.Level of Evidence2bLaryngoscope, 130:1640–1645, 2020
Impact of an Upper Respiratory Tract Infection on Botulinum Toxin Efficacy in Spasmodic Dysphonia Patients
Authors: Kirke Diana N. , Kaye Rachel , Blitzer Andrew 
Published: September 2019

ObjectiveTo determine whether the presence of a concomitant upper respiratory tract infection (URI) impacts upon Botulinum toxin (BoNT) efficacy in spasmodic dysphonia (SD) patients.Study DesignCase series and literature review.MethodsAll SD patients with a concurrent URI, presenting for BoNT therapy at a clinical research center from November 2016 to December 2017 were included. A total of 12 patients were identified. Patients were followed for at least two BoNT treatment cycles (approximately 6 months). The primary outcome measure was efficacy of the initial BoNT injection and the secondary outcome measure was the efficacy of the subsequent BoNT injection.ResultsAll subjects had adductor type SD (ADSD). There were 10 females and two males with a median age of 55 years (±19.5). All patients were well established on a consistent BoNT treatment regime, with an average administered dose of 1.0 unit (range 0.2–1.80 units). Bilateral injections were administered to 10 patients. Regarding the primary outcome measure, five failed to have any response to BoNT (41.7%), four had a partial response (33.3%), and three had a positive response to treatment (25.0%). When patients had their follow‐up injection in the absence of URI symptoms, 11 patients had a positive response to treatment (91.7%).ConclusionWhile the interplay between illness and BoNT efficacy is yet to be elucidated, we report that some patients are affected. We recommend that SD patients presenting for BoNT administration with a concomitant URI, should be counseled that their treatment might have decreased effect.Level of Evidence4 Laryngoscope, 130:1746–1749, 2020