bluuebunny/crossref_metadata_2025_split huggingface dataset:
Confinement of magnetism in atomically thin La0.7Sr0.3CrO3/La0.7Sr0.3MnO3 heterostructures
Authors: Koohfar Sanaz , Georgescu Alexandru B. , Penn Aubrey N. , LeBeau James M. , Arenholz Elke , Kumah Divine P. 
Published: May 2019

AbstractAt crystalline interfaces where a valence-mismatch exists, electronic, and structural interactions may occur to relieve the polar mismatch, leading to the stabilization of non-bulk-like phases. We show that spontaneous reconstructions at polar La0.7Sr0.3MnO3 interfaces are correlated with suppressed ferromagnetism for film thicknesses on the order of a unit cell. We investigate the structural and magnetic properties of valence-matched La0.7Sr0.3CrO3/La0.7Sr0.3MnO3 interfaces using a combination of high-resolution electron microscopy, first principles theory, synchrotron X-ray scattering and magnetic spectroscopy and temperature-dependent magnetometry. A combination of an antiferromagnetic coupling between the La0.7Sr0.3CrO3 and La0.7Sr0.3MnO3 layers and a suppression of interfacial polar distortions are found to result in robust long-range ferromagnetic ordering for ultrathin La0.7Sr0.3MnO3. These results underscore the critical importance of interfacial structural and magnetic interactions in the design of devices based on two-dimensional oxide magnetic systems.
Heterologous prime-boost with A(H5N1) pandemic influenza vaccines induces broader cross-clade antibody responses than homologous prime-boost
Authors: Levine Min Z. , Holiday Crystal , Jefferson Stacie , Gross F. Liaini , Liu Feng , Li Sheng , Friel Damien , Boutet Philippe , Innis Bruce L. , Mallett Corey P. , Tumpey Terrence M. , Stevens James , Katz Jacqueline M. 
Published: May 2019

AbstractHighly pathogenic avian influenza (HPAI) A(H5Nx) viruses continue to pose a pandemic threat. US national vaccine stockpiles are a cornerstone of the influenza pandemic preparedness plans. However, continual genetic and antigenic divergence of A(H5Nx) viruses requires the development of effective vaccination strategies using stockpiled vaccines and adjuvants for pandemic preparedness. Human sera collected from healthy adults who received either homologous (2 doses of a AS03A-adjuvanted A/turkey/Turkey/1/2005, A/Turkey), or heterologous (primed with AS03A-adjuvanted A/Indonesia/5/2005, A/Indo, followed by A/Turkey boost) prime-boost vaccination regimens were analyzed by hemagglutination inhibition and microneutralization assays against 8 wild-type HPAI A(H5Nx) viruses from 6 genetic clades. Molecular, structural and antigenic features of the A(H5Nx) viruses that could influence the cross-clade antibody responses were also explored. Compared with homologous prime-boost vaccinations, priming with a clade 2.1.3.2 antigen (A/Indo) followed by one booster dose of a clade 2.2.1 antigen (A/Turkey) administered 18 months apart did not compromise the antibody responses to the booster vaccine (A/Turkey), it also broadened the cross-clade antibody responses to several antigenically drifted variants from 6 heterologous clades, including an antigenically distant A(H5N8) virus (A/gyrfalcon/Washington/410886/2014, clade 2.3.4.4) that caused recent outbreaks in US poultry. The magnitude and breadth of the cross-clade antibody responses against emerging HPAI A(H5Nx) viruses are associated with genetic, structural and antigenic differences from the vaccine viruses and enhanced by the inclusion of an adjuvant. Heterologous prime-boost vaccination with AS03A adjuvanted vaccine offers a vaccination strategy to use existing stockpiled vaccines for pandemic preparedness against new emerging HPAI A(H5Nx) viruses.
Longitudinal multi-omics of host–microbe dynamics in prediabetes
Authors: Zhou Wenyu , Sailani M. Reza , Contrepois Kévin , Zhou Yanjiao , Ahadi Sara , Leopold Shana R. , Zhang Martin J. , Rao Varsha , Avina Monika , Mishra Tejaswini , Johnson Jethro , Lee-McMullen Brittany , Chen Songjie , Metwally Ahmed A. , Tran Thi Dong Binh , Nguyen Hoan , Zhou Xin , Albright Brandon , Hong Bo-Young , Petersen Lauren , Bautista Eddy , Hanson Blake , Chen Lei , Spakowicz Daniel , Bahmani Amir , Salins Denis , Leopold Benjamin , Ashland Melanie , Dagan-Rosenfeld Orit , Rego Shannon , Limcaoco Patricia , Colbert Elizabeth , Allister Candice , Perelman Dalia , Craig Colleen , Wei Eric , Chaib Hassan , Hornburg Daniel , Dunn Jessilyn , Liang Liang , Rose Sophia Miryam Schüssler-Fiorenza , Kukurba Kim , Piening Brian , Rost Hannes , Tse David , McLaughlin Tracey , Sodergren Erica , Weinstock George M. , Snyder Michael 
Published: May 2019

AbstractType 2 diabetes mellitus (T2D) is a growing health problem, but little is known about its early disease stages, its effects on biological processes or the transition to clinical T2D. To understand the earliest stages of T2D better, we obtained samples from 106 healthy individuals and individuals with prediabetes over approximately four years and performed deep profiling of transcriptomes, metabolomes, cytokines, and proteomes, as well as changes in the microbiome. This rich longitudinal data set revealed many insights: first, healthy profiles are distinct among individuals while displaying diverse patterns of intra- and/or inter-personal variability. Second, extensive host and microbial changes occur during respiratory viral infections and immunization, and immunization triggers potentially protective responses that are distinct from responses to respiratory viral infections. Moreover, during respiratory viral infections, insulin-resistant participants respond differently than insulin-sensitive participants. Third, global co-association analyses among the thousands of profiled molecules reveal specific host–microbe interactions that differ between insulin-resistant and insulin-sensitive individuals. Last, we identified early personal molecular signatures in one individual that preceded the onset of T2D, including the inflammation markers interleukin-1 receptor agonist (IL-1RA) and high-sensitivity C-reactive protein (CRP) paired with xenobiotic-induced immune signalling. Our study reveals insights into pathways and responses that differ between glucose-dysregulated and healthy individuals during health and disease and provides an open-access data resource to enable further research into healthy, prediabetic and T2D states.
Copper sulfide nanoparticles as high-performance cathode materials for Mg-ion batteries
Authors: Kravchyk Kostiantyn V. , Widmer Roland , Erni Rolf , Dubey Romain J.-C. , Krumeich Frank , Kovalenko Maksym V. , Bodnarchuk Maryna I. 
Published: May 2019

AbstractRechargeable magnesium batteries are appealing as safe, low-cost systems with high-energy-density storage that employ predominantly dendrite-free magnesium metal as the anode. While significant progress has been achieved with magnesium electrolytes in recent years, the further development of Mg-ion batteries, however, is inherently limited by the lack of suitable cathode materials, mainly due to the slow diffusion of high-charge-density Mg-ions in the intercalation-type host structures and kinetic limitations of conversion-type cathodes that often causes poor cyclic stability. Nanostructuring the cathode materials offers an effective means of mitigating these challenges, due to the reduced diffusion length and higher surface areas. In this context, we present the highly reversible insertion of Mg-ions into nanostructured conversion-type CuS cathode, delivering high capacities of 300 mAh g−1 at room temperature and high cyclic stability over 200 cycles at a current density of 0.1 A g−1 with a high coulombic efficiency of 99.9%. These materials clearly outperform bulk CuS, which is electrochemically active only at an elevated temperature of 50 °C. Our results not only point to the important role of nanomaterials in the enhancement of the kinetics of conversion reactions but also suggest that nanostructuring should be used as an integral tool in the exploration of new cathodes for multivalent, i.e., (Mg, Ca, Al)-ion batteries.
Climate controls on snow reliability in French Alps ski resorts
Authors: Spandre P. , François H. , Verfaillie D. , Lafaysse M. , Déqué M. , Eckert N. , George E. , Morin S. 
Published: May 2019

AbstractSki tourism is a major sector of mountain regions economy, which is under the threat of long-term climate change. Snow management, and in particular grooming and artificial snowmaking, has become a routine component of ski resort operations, holding potential for counteracting the detrimental effect of natural snow decline. However, conventional snowmaking can only operate under specific meteorological conditions. Whether snowmaking is a relevant adaptation measure under future climate change is a widely debated issue in mountainous regions, with major implications on the supply side of this tourism industry. This often lacks comprehensive scientific studies for informing public and private decisions in this sector. Here we show how climate change influences the operating conditions of one of the main ski tourism markets worldwide, the French Alps. Our study addresses snow reliability in 129 ski resorts in the French Alps in the 21st century, using a dedicated snowpack model explicitly accounting for grooming and snowmaking driven by a large ensemble of adjusted and downscaled regional climate projections, and using a geospatial model of ski resorts organization. A 45% snowmaking fractional coverage, representative of the infrastructures in the early 2020s, is projected to improve snow reliability over grooming-only snow conditions, both during the reference period 1986–2005 and below 2 °C global warming since pre-industrial. Beyond 3 °C of global warming, with 45% snowmaking coverage, snow conditions would become frequently unreliable and induce higher water requirements.
Systemic and intrathecal immune activation in association with cerebral and cognitive outcomes in paediatric HIV
Authors: Blokhuis C. , Peeters C. F. W. , Cohen S. , Scherpbier H. J. , Kuijpers T. W. , Reiss P. , Kootstra N. A. , Teunissen C. E. , Pajkrt D. 
Published: May 2019

AbstractDespite treatment, immune activation is thought to contribute to cerebral injury in children perinatally infected with human immunodeficiency virus (HIV). We aimed to characterize immune activation in relation to neuroimaging and cognitive outcomes. We therefore measured immunological, coagulation, and neuronal biomarkers in plasma and cerebrospinal fluid (CSF) samples of 34 perinatally HIV-infected children aged 8–18 years, and in plasma samples of 37 controls of comparable age, sex, ethnicity, and socio-economic status. We then compared plasma biomarker levels between groups, and explored associations between plasma/CSF biomarkers and neuroimaging and cognitive outcomes using network analysis. HIV-infected children showed higher plasma levels of C-reactive protein, interferon-gamma, interferon-gamma-inducible protein-10, and monocyte chemoattractant protein-1 than controls. In HIV-infected participants, plasma soluble CD14 was positively associated with microstructural white matter (WM) damage, and plasma D-dimer was negatively associated with WM blood flow. In CSF, IL-6 was negatively associated with WM volume, and neurofilament heavy-chain (NFH) was negatively associated with intelligence quotient and working memory. These markers of ongoing inflammation, immune activation, coagulation, and neuronal damage could be used to further evaluate the pathophysiology and clinical course of cerebral and cognitive deficits in perinatally acquired HIV.
Varying levels of X chromosome coalescence in female somatic cells alters the balance of X-linked dosage compensation and is implicated in female-dominant systemic lupus erythematosus
Authors: Laskowski Agnieszka I. , Neems Daniel S. , Laster Kyle , Strojny-Okyere Chelsee , Rice Ellen L. , Konieczna Iwona M. , Voss Jessica H. , Mathew James M. , Leventhal Joseph R. , Ramsey-Goldman Rosalind , Smith Erica D. , Kosak Steven T. 
Published: May 2019

AbstractThe three-dimensional organization of the genome in mammalian interphase nuclei is intrinsically linked to the regulation of gene expression. Whole chromosome territories and their encoded gene loci occupy preferential positions within the nucleus that changes according to the expression profile of a given cell lineage or stage. To further illuminate the relationship between chromosome organization, epigenetic environment, and gene expression, here we examine the functional organization of chromosome X and corresponding X-linked genes in a variety of healthy human and disease state X diploid (XX) cells. We observe high frequencies of homologous chromosome X colocalization (or coalescence), typically associated with initiation of X-chromosome inactivation, occurring in XX cells outside of early embryogenesis. Moreover, during chromosome X coalescence significant changes in Xist, H3K27me3, and X-linked gene expression occur, suggesting the potential exchange of gene regulatory information between the active and inactive X chromosomes. We also observe significant differences in chromosome X coalescence in disease-implicated lymphocytes isolated from systemic lupus erythematosus (SLE) patients compared to healthy controls. These results demonstrate that X chromosomes can functionally interact outside of embryogenesis when X inactivation is initiated and suggest a potential gene regulatory mechanism aberration underlying the increased frequency of autoimmunity in XX individuals.
Assessing the freshwater flux from the continents to the Mediterranean Sea
Authors: Wang Fuxing , Polcher Jan 
Published: May 2019

AbstractPrecipitation minus evaporation over continents is the freshwater flux which can be sustainably exploited by mankind. Over a catchment and longer time periods, this flux is also the amount of water which flows into the ocean. An essential question for semi-arid areas of the world is how well this freshwater flux can be estimated and predicted to evolve under climate change and human water use. Knowing this flux is thus an essential element in regional water resources management. Here we examine this question over the catchment of the Mediterranean Sea. Using a novel data assimilation methodology that incorporates observed discharges of rivers in a land surface model, new estimates of the freshwater flux to the Mediterranean Sea for the period 1980–2013 are proposed. We find that more freshwater (40–60%) flows into the sea than previously estimated. The hypothesis we advance is that previous estimates have underestimated the discharges of the large number of unmonitored coastal basins and neglected submarine ground water flows. The proposed error bars on the estimate indicate that the degrading river gauging station network limits our ability to monitor this branch of the water cycle reliably. Nevertheless, the uncertainty is small enough to allow the identification of regions in which non-climatic decreases in the freshwater flows exist over the period.
Generation of new hair cells by DNA methyltransferase (Dnmt) inhibitor 5-azacytidine in a chemically-deafened mouse model
Authors: Deng Xin , Liu Zhenjie , Li Xiaoyang , Zhou Yang , Hu Zhengqing 
Published: May 2019

AbstractRegeneration of mature mammalian inner ear hair cells remains to be a challenge. This study aims to evaluate the ability of DNA methyltransferase (Dnmt) inhibitor 5-azacytidine (5-aza) to generate outer hair cells (OHCs) in a chemically-deafened adult mouse model. 5-aza was administrated into the mouse inner ear via the round window. Immunofluorescence was used to examine the expression of hair cell specific proteins following 5-aza treatment. The results showed that in the chemically-deafened mouse cochlea, new OHCs were found post 5-aza treatment, whereas OHCs were completely lost in saline-treated mice. New hair cells expressed multiple hair cell markers included Myosin VIIa, Pou4f3 and Myosin VI. Newly-generated hair cells presented in three cochlear turns and were able to survive for at least six weeks. The effects of new hair cells generation by 5-aza were concentration dependent. Quantitative PCR study indicates that 5-aza may function through Dnmt1 inhibition. The results of this report suggest that the Dnmt inhibitor 5-aza may promote hair cell regeneration in a chemically-deafened mouse model.
Intermittent parathyroid hormone 1–34 induces oxidation and deterioration of mineral and collagen quality in newly formed mandibular bone
Authors: Yoshioka Yohsuke , Yamachika Eiki , Nakanishi Makoto , Ninomiya Tadashi , Akashi Sho , Kondo Sei , Moritani Norifumi , Kobayashi Yasuhiro , Fujii Tatsuo , Iida Seiji 
Published: May 2019

AbstractIntermittent parathyroid hormone (PTH) administration is known to promote bone healing after surgical procedures. However, the mechanism and influence of PTH on the mineral and collagen quality of the jaw are not well understood. Most studies have focused on analyzing the bone density and microstructure of the mandible, and have insufficiently investigated its mineral and collagen quality. Oxidative stress activates osteoclasts, produces advanced glycation end products, and worsens mineral and collagen quality. We hypothesized that PTH induces oxidation and affects the mineral and collagen quality of newly formed mandibular bone. To test this, we examined the mineral and collagen quality of newly formed mandibular bone in rats administered PTH, and analyzed serum after intermittent PTH administration to examine the degree of oxidation. PTH administration reduced mineralization and worsened mineral and collagen quality in newly formed bone. In addition, total anti-oxidant capacity in serum was significantly decreased and the oxidative-INDEX was increased among PTH-treated compared to vehicle-treated rats, indicating serum oxidation. In conclusion, intermittent administration of PTH reduced mineral and collagen quality in newly formed mandibular bone. This effect may have been induced by oxidation.
Metabolomics reveals diet-derived plant polyphenols accumulate in physiological bone
Authors: Alldritt Isabelle , Whitham-Agut Beatrice , Sipin Miguel , Studholme Jacob , Trentacoste Angela , Tripp Jennifer A. , Cappai Maria Grazia , Ditchfield Peter , Devièse Thibaut , Hedges Robert E. M. , McCullagh James S. O. 
Published: May 2019

AbstractPlant-derived secondary metabolites consumed in the diet, especially polyphenolic compounds, are known to have a range of positive health effects. They are present in circulation after ingestion and absorption and can be sequestered into cells within particular organs, but have rarely been investigated systematically in osteological tissues. However, a small number of polyphenols and similar molecules are known to bind to bone. For example alizarin, a plant derived anthraquinone and tetracycline (a naturally occurring antibiotic), are both absorbed into bone from circulation during bone formation and are used to monitor mineralization in osteological studies. Both molecules have also been identified serendipitously in archaeological human bones derived from natural sources in the diet. Whether an analogous mechanism of sequestration extends to additional diet-derived plant-polyphenols has not previously been systematically studied. We investigated whether a range of diet-derived polyphenol-like compounds bind to bone using untargeted metabolomics applied to the analysis of bone extracts from pigs fed an acorn-based diet. We analysed the diet which was rich in ellagitannins, extracts from the pig bones and surrounding tissue, post-mortem. We found direct evidence of multiple polyphenolic compounds in these extracts and matched them to the diet. We also showed that these compounds were present in the bone but not surrounding tissues. We also provide data showing that a range of polyphenolic compounds bind to hydroxyapatite in vitro. The evidence for polyphenol sequestration into physiological bone, and the range and specificity of polyphenols in human and animal diets, raises intriguing questions about potential effects on bone formation and bone health. Further studies are needed to determine the stability of the sequestered molecules post-mortem but there is also potential for (palaeo)dietary reconstruction and forensic applications.
Engineering the vasculature of decellularized rat kidney scaffolds using human induced pluripotent stem cell-derived endothelial cells
Authors: Ciampi Osele , Bonandrini Barbara , Derosas Manuela , Conti Sara , Rizzo Paola , Benedetti Valentina , Figliuzzi Marina , Remuzzi Andrea , Benigni Ariela , Remuzzi Giuseppe , Tomasoni Susanna 
Published: May 2019

AbstractGenerating new kidneys using tissue engineering technologies is an innovative strategy for overcoming the shortage of donor organs for transplantation. Here we report how to efficiently engineer the kidney vasculature of decellularized rat kidney scaffolds by using human induced pluripotent stem cell (hiPSCs)-derived endothelial cells (hiPSC-ECs). In vitro, hiPSC-ECs responded to flow stress by acquiring an alignment orientation, and attached to and proliferated on the acellular kidney sections, maintaining their phenotype. The hiPSC-ECs were able to self-organize into chimeric kidney organoids to form vessel-like structures. Ex vivo infusion of hiPSC-ECs through the renal artery and vein of acellular kidneys resulted in the uniform distribution of the cells in all the vasculature compartments, from glomerular capillaries to peritubular capillaries and small vessels. Ultrastructural analysis of repopulated scaffolds through transmission and scanning electron microscopy demonstrated the presence of continuously distributed cells along the vessel wall, which was also confirmed by 3D reconstruction of z-stack images showing the continuity of endothelial cell coverage inside the vessels. Notably, the detection of fenestrae in the endothelium of glomerular capillaries but not in the vascular capillaries was clear evidence of site-specific endothelial cell specialisation.
Top-down Effects on Empathy for Pain in Adults with Autistic Traits
Authors: Meng Jing , Shen Lin , Li Zuoshan , Peng Weiwei 
Published: May 2019

AbstractWhile empathic responses of individuals with autism-spectrum disorder have been reported to be modulated by top-down attention, it remains unclear whether empathy for pain in typically developing individuals with autistic traits also involves such top-down modulation mechanisms. This study employed the autism-spectrum quotient (AQ) to quantify autistic traits in a group of 1,231 healthy adults. Two subset groups (High-AQ and Low-AQ groups) were randomly selected from the highest and lowest 10% AQ scores respectively. We explored whether participants in both groups would differ in their response to others’ pain when their attention was directed toward (A-P tasks) or away (A-N tasks) from pain cues in auditory and visual experimental modalities. Compared to Low-AQ individuals, High-AQ individuals exhibited more suppressed N1 and P2 amplitudes in response to painful vocal cues in auditory A-N tasks. This suggests suppressed attentional and emotional processes of empathy for pain when High-AQ individuals have their attention directed away from others’ pain cues. No significant difference was found between both groups in the auditory A-P task, nor in the visual A-P and A-N tasks. These results suggest that top-down attention modulation of cortical empathic responses to others’ vocal pain is influenced by autistic traits.