bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development, validation, and application of a liquid chromatography–tandem mass spectrometry method for quantitative determination of trans-4-hydroxy-l-proline concentration in the serum of dogs with chronic hepatitis
Authors: Lawrence Yuri A. , Rodrigues-Hoffmann Aline , Steiner Jörg M. , Suchodolski Jan S. , Shankar Smriti , Klemashevich Cory L. , Lidbury Jonathan A. 
Published: April 2019

Abstract

OBJECTIVE
To develop and analytically validate a liquid chromatography–tandem mass spectrometry method for measurement of endogenous trans-4-hydroxy-l-proline concentrations in canine serum and to assess serum trans-4-hydroxy-l-proline concentrations in dogs with chronic hepatitis.

SAMPLE
Serum samples obtained from 20 dogs with histopathologically confirmed chronic hepatitis and 20 healthy control dogs.

PROCEDURES
A liquid chromatography–tandem mass spectrometry method for quantification of trans-4-hydroxy-l-proline concentration was developed and assessed for analytic sensitivity, linearity, accuracy, precision, and reproducibility. Serum concentration of trans-4-hydroxy-l-proline in dogs with chronic hepatitis and healthy control dogs was measured.

RESULTS
Observed-to-expected ratios for dilutional parallelism ranged from 72.7% to 111.5% (mean ± SD, 91.3 ± 19.6%). Intra-assay and interassay coefficients of variation ranged from 2.1% to 3.0% and 3.2% to 5.3%, respectively. Relative error ranged from −2.3% to 7.8%. Trans-4-hydroxy-l-proline concentrations were significantly lower in serum obtained from dogs with chronic hepatitis (median, 0.24 ng/mL; range, 0.06 to 1.84 ng/mL) than in serum obtained from healthy control dogs (median, 0.78 ng/mL; range, 0.14 to 4.90 ng/mL).

CONCLUSIONS AND CLINICAL RELEVANCE
The method described here for the quantification of trans-4-hydroxy-l-proline concentration in canine serum was found to be sensitive, specific, precise, accurate, and reproducible. Dogs with chronic hepatitis had significantly lower serum trans-4-hydroxy-l-proline concentrations than did healthy control dogs, possibly as a result of altered hepatic metabolism of amino acids.
Identification of reference genes for microRNAs of extracellular vesicles isolated from plasma samples of healthy dogs by ultracentrifugation, precipitation, and membrane affinity chromatography methods
Authors: Narita Momoko , Nishida Hidetaka , Asahina Ryota , Nakata Kohei , Yano Hirohito , Ueda Tomoyuki , Inden Masatoshi , Akiyoshi Hideo , Maeda Sadatoshi , Kamishina Hiroaki 
Published: April 2019

Abstract

OBJECTIVE
To compare ultracentrifugation, precipitation, and membrane affinity chromatography methods for isolation of extracellular vesicles (EVs) from canine plasma samples and to identify suitable reference genes for incorporation into a quantitative reverse transcription PCR assay of microRNA expression in plasma EVs of healthy dogs.

ANIMALS
6 healthy Beagles.

PROCEDURES
Plasma samples were obtained from each dog, and EVs were isolated from 0.3 mL of these samples via ultracentrifugation, precipitation, and membrane-affinity chromatographic methods. Nanoparticle tracking analysis was performed to determine the concentration and size distribution of EVs isolated by the ultracentrifugation method. Expression levels (cycle threshold values) of 4 microRNAs (let-7a, miR-16, miR-26a, and miR-103) were then compared by means of quantitative reverse transcription PCR assay. Three statistical programs were used to identify the microRNAs most suitable for use as reference genes.

RESULTS
Results indicated that ultracentrifugation was the most stable of all 3 methods for isolating microRNAs from 0.3 mL of plasma. Nanoparticle tracking revealed that EV samples obtained by the ultracentrifugation method contained a mean ± SD of approximately 1.59 × 1010 vesicles/mL ± 4.2 × 108 vesicles/mL. Of the 4 microRNAs in plasma EVs isolated by ultracentrifugation, miR-103 was the most stable.

CONCLUSIONS AND CLINICAL RELEVANCE
The ultracentrifugation method has potential as a stable method for isolating EVs from canine plasma samples with a high recovery rate, and miR-103 may provide the most stable reference gene for normalizing microRNA expression data pertaining to plasma EVs isolated by ultracentrifugation.
DILEMA INDEPENDENSI AUDITOR SATUAN PENGAWASAN INTERNAL PERGURUAN TINGGI: STUDI FENOMENOLOGI
Authors: Malle Syarif Syahrir , Alimudidin Alimudidin , Nirwana Nirwana 
Published: April 2019

This research is motivated by a "unique" phenomenon from of the role of the profession internal auditors. For Internal auditors of Internal Control Unit (SPI) in a non-profit organization, especially in an Higher Education Institution, the uniqueness has the potential to place it in a situation of independence dilemma. The purpose of this study is to understand the reality of the independence dilemma faced by SPI auditors as a result of the conditions and work environment. In order to explore the auditor's understanding of SPI, phenomenology is employed as methodology, under interpretive paradigm as the umbrella of this research. Exploration of understanding and awareness is done using Epoche. The results of the study revealed that the SPI auditor faced a situation of independence in various forms: (1) the negative auditing stigma in the form of the awarding of "kambing hitam" to SPI raises a dilemma for auditors and disturbs the working spirit of the SPI auditor, on the other hand if implemented improperly will be contrary to the obligation and further aggravate the negative stigma already attached; (2) there is a phenomenon of impropriety (abuse) on audit guidelines that has not been fully implemented and not evaluated to provide ethical consideration in the future; (3) the existence of obedience pressure in the form of "suspension" the rector signs the SPI examination report. due to the term audit conception that has not been agreed upon.
Protective role of eclipta alba against hyperlipidemia induced by high-fat diet in albino rats
Authors: Satheesh Naik K  , Gurushanthaiah M  , Nagesh Raju G  , Lokanadham S  , Seshadri Reddy V  
Published: April 2019

Eclipta Alba has been used in traditional and folklore medicine to treat Hyperlipidemia and hepatic disorders. The present study was aimed to investigate the Antihyperlipidemic and hepatoprotective potentials of Eclipta Alba in high-fat diet -induced Albino rats and to determine the underlying mechanism. A total of 30 adult albino rats of Wistar strain weighing 165–215 g were utilized. Animals were treated with high-fat diet for 8 weeks followed by post-treatment of E. Alba for 1 week, 2 weeks, and 3 weeks, respectively. After 12 h of fasting on the last day of the experiment, serum blood samples were collected in EDTA vials and processed for biochemical analysis. A significant decrease in levels of total cholesterol and triglycerides was noted on animals treated with E. alba compared to high-fat diet animals. Treatment of hypercholesterolemic rats with E. Alba showed a marked decrease of serum low-density lipoprotein (LDL) and very LDL cholesterol concentrations compared to the hypercholesterolemic rats. High-fat diet feeding worsened the levels of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, and alkaline phosphatase enzymes, whereas the same markers were significantly improved by supplementation with E. alba compared to the normal group. E. alba acts as an antihyperlipidemic agent in hyperlipidemic conditions and helps for better health.
COLONIALIDADE E EDUCAÇÃO:
Authors: Flor do Nascimento Wanderson , Botelho Denise 
Published: April 2019

O presente texto busca discutir o silenciamento de produções nacionais, latino-americanas e africanas nos currículos de filosofia brasileiros como um sintoma de uma internalização dos discursos coloniais que constituíram a maneira moderna de lidarmos com a filosofia, com a educação, com o ensino de filosofia. Algumas das principais categorias dos estudos sobre a colonialidade serão utilizadas como roteiro para essa discussão.
Rough Spaces on Covering Based Rough Sets
Authors: Alharbi Nof , Aydi Hassen , Ã–zel Cenap 
Published: April 2019

In this paper, we discuss open (closed) sets, rough interiors, rough closures, continuous mappings,Â open (closed) mappings and homeomorphism mappings ofÂ covering based rough topology of Akduman, Ã–zcelik and Ã–zel. We also construct the nano topology on a given covering based rough set.
Toleransi yang Canggung; Menyingkap Toleransi Beragama Kelompok Kristen di Samarinda
Authors: Ijal Syamsurijal 
Published: April 2019

Perspektif dan praktik toleransi agama Kristen selama ini dianggap bisa menjadi contoh dalam membangun relasi yang harmoni antara pemeluk agama yang berbeda. Pemeluk agama Kristen diasumsikan bisa menerima perbedaan agama dengan cukup baik. Landasannya adalah ajaran cinta kasih yang kemudian diimplementasikan dalam bentuk “mencintai untuk semua”. Namun acap kali landasan cinta kasih tidak bisa diterapkan dengan baik. Faktor di luar agama, seperti persoalan budaya, politik, dan ekonomi membuat ajaran cinta memiuh menjadi sikap yang intoleran. Penelitian yang bertujuan mengungkap perspektif dan praktik toleransi Agama Kristen dengan metode kualitatif ini, menemukan kasus perspektif dan praktik toleransi umat Kristen di Samarinda justru berjalan canggung. Di satu sisi mereka meyakini ajaran cinta dan kasih tapi dalam praktiknya mereka dibatasi oleh persoalan hubungan kuasa mayoritas-minoritas. Hal ini setidaknya terlihat pada Gereja Kemah Injil dan Saksi Yehuwa di Samarinda. Kedua kelompok ini tidak bisa membangun hubungan yang lebih dalam dan aktif dengan kelompok agama yang berbeda. Mereka merasa bahwa kelompok lain, khususnya kelompok agama mayoritas (muslim) membatasi berbagai ekspresi keagamaan mereka atas nama dominasi mayoritas. Jadilah perspektif dan praktik toleransinya tidak seideal yang dibayangakan. Saksi Yehuwa misalnya cenderung melihat kebenaran semata hanya pada dirinya. Sementara jemaat Gereja Kemah Injili kesulitan membangun hubungan yang lebih akrab dengan agama lain, khususnya Islam.
CORRELATION OF SERUM VITAMIN D LEVEL WITH MORTALITY IN PATIENTS WITH SEPSIS
Authors: Sood Pramod , Kaur Manpreet 
Published: April 2019

Background: Vitamin D insufficiency is a common global phenomenon. Its insufficiency is also reported to be common in hospitalized patients and particularly in critically ill patients.
Methods: Following ethical approval, consent will be sought from either the patient or assent from a near relative. Successive patients admitted to the medical emergency and ICU at tertiary care health center who fulfill the following criteria for sepsis, within a 24 h time window, were included in the study.
Results: A total of 50 patients were enrolled in the study, 37 (74.00%) were discharged whereas 13(26.00%) patients expired. The majority of patients, irrespective of outcome had Vitamin D deficiency. Although normal Vitamin D levels were observed in relatively higher proportion of patients who were discharged (29 patients) as compared to those who expired (12 patients) yet this difference was not significant statistically (P = 0.481).
Conclusion: Vitamin D deficiency leads to increased risk of mortality in the critically ill patients.
Keywords: Sepsis, Vitamin D deficiency, Mortality.
Street Children's Experience in Maintaining Reproductive Health
Authors: Kusumaningtiyas Dwi Prima Hanis , Dedi Blacius , Ropei Oop 
Published: April 2019

Introduction. Street children are children who spend most of their time doing day-to-day activities on the streets, including in the market environment, shops and other hubs. Street children are less concerned about their own health. Access to health services is good for children less than normal people. It affects their health behavior style. This study aims to deeply exploit the juvenile street children's experience in maintaining reproductive health in the Bahtera Child Protection Home Foundation Bandung City. Methods. This research is a qualitative research with phenomenology approach. Participants in this study are adolescent street children under the auspices of the Bahtera Child Protection Home Foundation Bandung City with a total of 7 people. Data collection by indepth interview. Data analysis using thematic analysis according to Collaizzi's method. Results. The research result are six themes; The experience of get reproductive health knowledge, Behavior of reproductive hygiene, Food intake, Drug dependence behavior, psychotropic, and addictive substances, Forms of social violence
Polityka konkurencji Unii Europejskiej w warunkach globalizacji gospodarki światowej
Authors: Latoszek Ewa , Kłos Agnieszka 
Published: April 2019

The aim of this article is to present the essence of competition policy and its implementation in the European Union in the context of ongoing globalization of the world economy. The paper will present selected factors that stimulate the process of globalization, main objectives and tools supporting the functioning of the EU internal market, and the place of the European Commission as a body that enforces compliance with the rules of competition by companies and the Member States.
Na ile innowacyjny jest eksport usług z Polski?
Authors: Czarny Elżbieta , Kuźnar  Andżelika , Śledziewska  Katarzyna 
Published: April 2019

We analyse Polish export of services, examining how innovative it is. We examine both the changes in the size of Polish exports of services as well as the importance of branches and geographical directions. We stress the relatively small economic and, consequently, export potential of Poland, as compared to the EU and the USA. At the same time, similar potentials of the EU and the USA, and different geographic distances and characteristics of their connections with Poland were the reason for comparing Poland's exports to both partners. To analyse Poland's total export of services, we add to the two previously mentioned trade partners a third group, which consists of relatively less developed countries. Consequently, the recipients of services from Poland are divided into the European Union, the United States and other trading partners defined as non-EU markets minus the USA. Due to absence of data and limited research framework, we limit the overall analysis to the years 2004-2013, while the detailed (industry) analysis to the years 2004-2012. In either case, the last year of the survey is also the last year for which annual Eurostat data is available.
External and internal liberalization that ENP promotes as transmission belts of democratization and political stability: success and failure revisited - the Southern Dimension
Authors: Zajaczkowski Małgorzata 
Published: April 2019

The European Neighborhood Policy (ENP) was launched in 2004 with the aim of building new political and economic relations between the EU and its Southern and Eastern neighboring states through support for introduction of reforms and systemic changes in the partner states. In the South, the initiative covered ten countries: Algeria, Egypt, Israel, Jordan, Lebanon, Libya, Morocco, Palestine, Syria and Tunisia. It was associated with better political and economic relations with the EU, which should result in a wider access to the European market for Mediterranean goods and a larger foreign direct investment in$ow to the partner countries' markets. The aim of the paper is to analyze the effects of ENP on the Southern Mediterranean neighbors in the context of economic liberalization and pro-democratic changes. The paper analyses two problems: economic development and trade liberalization offered by ENP through deeper integration with the EU and marketoriented regulations. The second problem analyzed in the paper concerns the process of democratic changes that the EU committed to promote. Due to the highly controversial nature of democratization for the Mediterranean states, it was replaced by the idea of good governance. For this purpose, general trends within the Mediterranean societies in their approach to that notion of governance are presented.
Cybergenic Presidential Candidates of Third Parties in the United States: The Analysis of Selected Political Campaigns with the Key Role of New Media
Authors: Popielec Dominika 
Published: April 2019

This article focuses on selected political campaigns of the Third Parties in the United States. The main purpose of this work is to present how these candidates promote themselves and communicate with voters. Traditional and new media are playing a crucial role in public life during presidential elections. Not only do they inform society but also are a tool of political communication. Voters can get to know a candidate and his/her election program via the media. Journalists are conducting interviews with main candidates, especially the Democrats and Republicans, which are leaders in the polls. But what should the candidates from other parties do? Those who do not appear very often in the mainstream media? How do they gain public support for their ideas? Do we insist on a telegenic president, as William F. Buckley indicated, or a cybergenic president in the contemporary world? Considering the increasing role of the Internet in modern society, these candidates use new new media to promote themselves. Therefore, in this article I will describe the importance of new media, their effectiveness in presidential campaigns and a cybergenic candidate as a standard of modern political communication
Międzynarodowa konkurencyjność sieci popytu - innowacje poprzez cloud computing
Authors: Nowicka Katarzyna 
Published: April 2019

In today's economy supply chains are frequently transforming into international demand networks. Cloud computing is the answer to the main challenges posed by creation and management of flexible and adaptive global demand networks. Cloud computing is a tool that forms a link between technology and business, and thus innovatively improves competitiveness of international supply chains while supporting development of individual economic entities regardless of their size and scale of activities. Cloud computing is the basis for the development of the economy of sharing, mass customization, social networks, systems infrastructure and communications of individual companies and those of international supply chains. The article presents the possibilities of using cloud computing in supporting development of innovation in management of international demand networks. The article elaborates on whether cloud computing is an innovation, or rather just a tool to introduce innovations and what is the extent of the impact of its use on the level of innovation in international demand networks
Kreowanie zielonych miejsc pracy w polskim sektorze energetyki odnawialnej
Authors: Kryk Barbara 
Published: April 2019

The concept of green jobs began to spread with the development of the theory of green economy, which is conceptually linked to the idea of sustainable development. Its introduction requires structural changes, including the creation of green jobs. Green jobs (colloquially referred to as green-collar jobs) exist in various sections and sectors. Their creation is important from an economic, ecological and social point of view. Hence, the aim of this article is to evaluate the development of green jobs in Poland in the years 2010–2015 against the EU, with an emphasis on renewable energy. In the study the author used desk research and indicator analysis for changes in the number, structure and dynamics of green jobs to demonstrate progress in their creation and in meeting Poland’s commitments under the Europe 2020 strategy. Although the study has shown positive changes in green employment, they are insufficient.
Foreign Direct Investment in Real Estate and Related Business Services:Poland vs OECD Countries​
Authors: Humanicki Marcin , Olszewski Krzysztof 
Published: April 2019

Poland has experienced a relatively large inflow of foreign direct investment (FDI) in the last two decades. FDI has been flowing mainly into services and manufacturing similarly to the trend observed in global capital movement. Within the services, the financial intermediation played the biggest role in terms of attracting FDI, but real estate and related business activities (legal, accounting, auditing, tax, marketing, etc.) also saw a high inflow of foreign capital. The paper analyzes the evolution of inward FDI stock in Poland over the period 1998–2012 with a particular emphasis on real estate and related business activities. It also discusses how Poland differs in terms of sectoral decomposition in the services of inward FDI stock from other Organization for Economic Cooperation and Development (OECD) countries representing both Central and Eastern Europe (CEE) and advanced economies. Finally, the paper answers the question how Poland is performing relative to other OECD members in terms of FDI attractiveness.
IDENTIFIKASI KOMPONEN PENDUKUNG DAYA TARIK WISATA LOANG BALOQ SEBAGAI WISATA PESISIR DI KOTA MATARAM
Authors: Nugroho M Setyo 
Published: April 2019

Daya tarik wisata Loang Baloq di Kota Mataram memiliki diversifikasi produk wisata yang terbagi menjadi tiga area, yakni darat, laut dan muara. Namun secara praktiknya, daya tarik wisata Loang Baloq hanya dikenal oleh wisatawan lokal, tidak demikian untuk wisatawan mancanegara dan nusantara, sehingga manfaat pariwisata belum sepenuhnya dirasakan oleh masyarakat lokal. Penelitian ini bertujuan untuk mengidentifikasi komponen pendukung daya tarik wisata Loang Baloq di Kota Mataram. Data dikumpulkan dengan teknik observasi, wawancara (purposive sampling) dan studi dokumentasi. Teknik analisis data menggunakan deskriptif kualitatif. Hasil penelitian ini menunjukkan bahwa DTW Loang baloq memiliki keunikan dan ciri khas atraksi alam dan budaya sebagai modal dasar untuk menarik wisatawan doemestik dan mancanegara. Namun masih terdapat permasalahan yang menjadi penghambat perkembangan daya tarik wisata, seperti rendahnya SDM, manajemen pengelolaan sampah yang kurang baik dan fasilitas yang rusak.
Quelques aspects remarquables de la météorite achondritique de Malvern (Afrique du Sud)
Authors: Jérôme Dominique Yves , Christophe Michel-Lévy Mireille 
Published: April 2019

The chemical analyses of major elements suggest that this achondrite belongs to the howardites.
However in addition to interesting lithic fragments, the presence of glasses in numerous debris up to several millimeters and in spherules around 50 µm in diameter has been found in the course of a preliminary mineralogical study. By these charactetistics the Malvern meteorite resembles lunar breccias more than howardites.
La permingeatite Cu2Sb Se4, un nouveau minéral du groupe de la luzonite
Authors: Johan Zdenek , Picot Paul , Pierrot Roland , Kvacek Milan 
Published: April 2019

La permingeatite, Cu₃SbSe₄, un nouveau séléniure du groupe de la luzonite et de la germanite a été trouvé à Predborice, Bohême, Tchécoslovaquie. Quadratique, D¹¹/2d — I[-4]2 m, a = 5,63, c = 11,23 Å, c/a = 1,994, Z = 2, dₓ = 5,82.
Les raies les plus intenses du diagramme de poudre sont les suivantes : 3,251 (10) ; 1,986 (9) ; 1,697 (8) ; 1,290 (6) ; 1,148 (7) Å. Analyse chimique (microsonde électronique) : Cu — 30,7 ; Sb — 17,4 ; As — 1,5 ; Se — 50,4 ; total 100,0. En section polie, elle est brun rose clair, fortement anisotrope. Macles en lamelles monosynthétiques. Microdureté (Vickers) 234 kg/mm². Pouvoir réflecteur : 28,8-30,4 (420 nm) ; 27.9-27,9 (500 nm) ; 25,4-25,2 (600 nm) ; 23,8-25,4 (640 nm). Trouvée dans un filon de calcite avec berzelianite, hakite, eskebornite, umangite, clausthalite, ferroselite, klockmannite, or natif, pechblende, hématite, goethite, chalcopyrite, pyrite et d'autres minéraux non encore identifiés. Dédiée à François Permingeat.