bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of optical projection tomography and light‐sheet fluorescence microscopy
Authors: LIU A. , XIAO W. , LI R. , LIU L. , CHEN L. 
Published: April 2019

SummaryWe present a numerical analysis and experimental characterisation of spatial resolution in optical projection tomography (OPT) and light‐sheet fluorescence microscopy (LSFM) using their ‘standard’ systems. Although both techniques provide spatial resolution at the micrometre scale for mesoscopic (millimetre to centimetre) samples, LSFM provides higher lateral (∼3 μm, ∼34% of OPT) but lower axial (∼25.8 μm, 295% of OPT) resolution as compared to OPT (∼8.75 μm, 100%) when imaging the same sample (∼2 mm). Moreover, OPT provides isotropic spatial resolution due to its rotational scanning which may reduce the ambiguity in 3D analysis, so it is more practically appropriate for relatively large samples. We also demonstrate the application performances of both techniques by imaging various biological tissues, illustrating their imaging ability at different spatial scales.Lay DescriptionOptical projection tomography (OPT) and light‐sheet fluorescence microscopy (LSFM) are generally used to extract 3D information from relatively large biological tissues/organs/embryos or even some small animals. Both techniques have made a great progress in recent decades and have been widely applied in life science, medical research and so on. The different implementation features of these two techniques results in isotropic and anisotropic spatial resolution respectively, making a dilemma for the researchers to choose the appropriate system when imaging the samples with different size. So far, there is no study to numerically discuss the differences between their image formation properties and to adequately quantify their own strengths and limitations.In our work, we quantified the imaging behaviour in ‘standard’ OPT and LSFM using both numerical analysis and experimental characterisations, showing the relationship between spatial resolution and sample size in each system. We also demonstrated the detailed structure differences when imaging various biological tissues. We believe this work will be useful and can provide a reference for the 3D fluorescence‐imaging‐based researchers.
TEM diffraction contrast images simulation of dislocations
Authors: WU W. , SCHAEUBLIN R. 
Published: April 2019

SummaryFor end‐on screw dislocations and inclined dislocations within thin transmission electron microscopy (TEM) foil, TEM diffraction contrast image is largely modified around piercing point due to free surface relaxation. Based on many‐beam Schaeublin–Stadelmann equations, TEM diffraction contrast images simulation of inclined dislocations within thin pure Fe TEM foil are performed and the difference between isotropic and anisotropic dislocation models are studied. Image force are superposed onto bulk elastic field of dislocation in thin foil, the elastic distortions nearby the two emerging points of a straight inclined dislocation located in an elastically isotropic/anisotropic thin foil are expressed as semianalytical solution in Fourier space, and the semianalytical image stress solutions in Fourier space are implemented into CUFOUR for studying free surface relaxation effect on TEM diffraction contrast of dislocation. Simulation results suggest that the diffraction contrast at the ends of dislocation is modified drastically by image forces, and effects of anisotropy cannot be neglected.Lay DescriptionFor end‐on screw dislocations and inclined dislocations within thin transmission electron microscopy (TEM) foil, TEM diffraction contrast image is largely modified around piercing point due to free surface relaxation. Based on many‐beam Schaeublin–Stadelmann equations, TEM diffraction contrast images simulation of inclined dislocations within thin pure Fe TEM foil are performed and the difference between isotropic and anisotropic dislocation models are studied. Image force are superposed onto bulk elastic field of dislocation in thin foil, the elastic distortions nearby the two emerging points of a straight inclined dislocation located in an elastically isotropic/anisotropic thin foil are expressed as semianalytical solution in Fourier space, and the semianalytical image stress solutions in Fourier space are implemented into CUFOUR for studying free surface relaxation effect on TEM diffraction contrast of dislocation. Simulation results suggest that the diffraction contrast at the ends of dislocation is modified drastically by image forces, and effects of anisotropy cannot be neglected.
Oral health‐related quality of life, oral aesthetics and oral function in head and neck cancer patients after oral rehabilitation
Authors: Fromm Lærke , Gotfredsen Klaus , Wessel Irene , Øzhayat Esben Boeskov 
Published: April 2019

AbstractHead and neck cancer (HNC) is diagnosed in more than 500 000 patients every year worldwide with increasing prevalence. Oral rehabilitation is often needed after HNC treatment to regain oral function, aesthetics and oral health‐related quality of life (OHRQoL). The objectives were to evaluate OHRQoL, oral aesthetics and oral function after oral rehabilitation in HNC patients and compare it to that of non‐HNC patients. Eighteen patients treated for HNC who subsequently had oral rehabilitation (2014‐2017), and a control group of eighteen age‐ and gender‐matched non‐HNC patients treated with removable prostheses (2014‐2018) were included in a cross‐sectional study. The OHRQoL was assessed by the Oral Health Impact Profile 49 questionnaire (OHIP‐49), the oral aesthetics by the Prosthetic Esthetic Index (PEI) and the Orofacial Esthetic Scale (OES), and the oral function by the Nordic Orofacial Test‐Screening (NOT‐S). The HNC patients had worse oral function and OHRQoL than the control patients (mean NOT‐S score 4.56 vs 0.56, P < 0.01 and mean OHIP‐49 score 42.50 vs 20.94, P = 0.050). When including number of replaced teeth and type of prosthesis in the tests, no significant difference in OHRQoL was found between the groups. No difference was found in the overall aesthetic outcomes (mean PEI total score 32.28 vs 30.67, P = 0.367 and mean OES total score 48.78 vs 53.56, P = 0.321). Multiple regression analyses showed that being HNC patient compared to control patient impaired the oral function. Oral function is significantly impaired in HNC patients compared to non‐HNC patients after oral rehabilitation.
Impact of feeding method on overall activity of indoor, client‐owned dogs
Authors: Su D. K. , Murphy M. , Hand A. , Zhu X. , Witzel‐Rollins A. 
Published: April 2019

ObjectiveTo compare the total daily activity time, walking time and running time using food‐dispensing toys versus bowls in a group of client‐owned, primarily indoor dogs.Materials and MethodsA two‐way, two period, randomised repeated measures mixed‐effects crossover study performed on 26 client‐owned, primarily indoor dogs.ResultsToy feeding increased average daily total activity time by 12% and walking time by 26%. Average daily total activity time and walking time were reduced by 8 and 7% respectively with each increase in year of age. Gender, body condition and muscle condition had no significant effect on average daily total activity or walking time. Toy feeding, time, their interaction, age, gender, body condition and muscle condition had no significant effect on average daily running time.Clinical SignificanceFeeding toys may be helpful during weight loss programs to achieve the goal of increasing daily exercise duration in dogs that need to lose weight.
The return of the frogs: The importance of habitat refugia in maintaining diversity during a disease outbreak
Authors: McKnight Donald T. , Lal Monal M. , Bower Deborah S. , Schwarzkopf Lin , Alford Ross A. , Zenger Kyall R. 
Published: April 2019

AbstractRecent decades have seen the emergence and spread of numerous infectious diseases, often with severe negative consequences for wildlife populations. Nevertheless, many populations survive the initial outbreaks, and even undergo recoveries. Unfortunately, the long‐term effects of these outbreaks on host population genetics are poorly understood; to increase this understanding, we examined the population genetics of two species of rainforest frogs (Litoria nannotis and Litoria serrata) that have largely recovered from a chytridiomycosis outbreak at two national parks in the Wet Tropics of northern Australia. At the wetter, northern park there was little evidence of decreased genetic diversity in either species, and all of the sampled sites had high minor allele frequencies (mean MAF = 0.230–0.235), high heterozygosity (0.318–0.325), and few monomorphic markers (1.4%–4.0%); however, some recovered L. nannotis populations had low Ne values (59.3–683.8) compared to populations that did not decline during the outbreak (1,537.4–1,756.5). At the drier, southern park, both species exhibited lower diversity (mean MAF = 0.084–0.180; heterozygosity = 0.126–0.257; monomorphic markers = 3.7%–43.5%; Ne = 18.4–676.1). The diversity patterns in this park matched habitat patterns, with both species having higher diversity levels and fewer closely related individuals at sites with higher quality habitat. These patterns were more pronounced for L. nannotis, which has lower dispersal rates than L. serrata. These results suggest that refugia with high quality habitat are important for retaining genetic diversity during disease outbreaks, and that gene flow following disease outbreaks is important for re‐establishing diversity in populations where it was reduced.
Facilitators and barriers to teaching undergraduate medical students in general practice
Authors: Barber John Richard George , Park Sophie Elizabeth , Jensen Kim , Marshall Hannah , McDonald Paula , McKinley Robert Kee , Randles Hannah , Alberti Hugh 
Published: April 2019

ContextGlobally, primary health care is facing workforce shortages. Longer and higher‐quality placements in primary care increase the likelihood of medical students choosing this specialty. However, the recruitment and retention of community primary care teachers are challenging. Relevant research was predominantly carried out in the 1990s. We seek to understand contemporary facilitators and barriers to general practitioner (GP) engagement with undergraduate education. Communities of practice (CoP) theory offers a novel conceptualisation, which may be pertinent in other community‐based teaching settings.MethodsSemi‐structured interviews were undertaken with 24 GP teachers at four UK medical schools. We purposively sampled GPs new to teaching, established GP teachers and GPs who had recently stopped teaching. We undertook NVivo‐assisted deductive and inductive thematic analysis of transcripts. We used CoP theory to interpret data.ResultsCommunities of practice theory illustrated that teachers negotiate membership of three CoPs: (i) clinical practice; (ii) the medical school, and (iii) teaching. The delivery of clinical care and teaching may be integrated or exist in tension. This can depend upon the positioning of the teaching and teacher as central or peripheral to the clinical CoP. Remuneration, workload, space and the expansion of GP trainee numbers impact on this. Teachers did not identify strongly as members of the medical school or a teaching community. Perceptions of membership were affected by medical school communication and support. The findings demonstrate gaps in medical school recruitment.ConclusionsThis research demonstrates the marginalisation of primary care‐based teaching and proposes a novel explanation rooted in CoP theory. Concepts including identity and membership may be pertinent to other community‐based teaching settings. We recommend that medical schools review and broaden recruitment methods. Teacher retention may be improved by optimising the interface between medical schools and teachers, fostering a teaching community, increasing professional rewards for teaching involvement and altering medical school expectations of learning in primary care.
NbALD1 mediates resistance to turnip mosaic virus by regulating the accumulation of salicylic acid and the ethylene pathway in Nicotiana benthamiana
Authors: Wang Shu , Han Kelei , Peng Jiejun , Zhao Jinping , Jiang Liangliang , Lu Yuwen , Zheng Hongying , Lin Lin , Chen Jianping , Yan Fei 
Published: April 2019

SummaryAGD2‐LIKE DEFENCE RESPONSE PROTEIN 1 (ALD1) triggers plant defence against bacterial and fungal pathogens by regulating the salicylic acid (SA) pathway and an unknown SA‐independent pathway. We now show that Nicotiana benthamiana ALD1 is involved in defence against a virus and that the ethylene pathway also participates in ALD1‐mediated resistance. NbALD1 was up‐regulated in plants infected with turnip mosaic virus (TuMV). Silencing of NbALD1 facilitated TuMV infection, while overexpression of NbALD1 or exogenous application of pipecolic acid (Pip), the downstream product of ALD1, enhanced resistance to TuMV. The SA content was lower in NbALD1‐silenced plants and higher where NbALD1 was overexpressed or following Pip treatments. SA mediated resistance to TuMV and was required for NbALD1‐mediated resistance. However, on NahG plants (in which SA cannot accumulate), Pip treatment still alleviated susceptibility to TuMV, further demonstrating the presence of an SA‐independent resistance pathway. The ethylene precursor, 1‐aminocyclopropanecarboxylic acid (ACC), accumulated in NbALD1‐silenced plants but was reduced in plants overexpressing NbALD1 or treated with Pip. Silencing of ACS1, a key gene in the ethylene pathway, alleviated the susceptibility of NbALD1‐silenced plants to TuMV, while exogenous application of ACC compromised the resistance of Pip‐treated or NbALD1 transgenic plants. The results indicate that NbALD1 mediates resistance to TuMV by positively regulating the resistant SA pathway and negatively regulating the susceptible ethylene pathway.
Leadership for public value: Political astuteness as a conceptual link
Authors: Hartley Jean , Sancino Alessandro , Bennister Mark , Resodihardjo Sandra L. 
Published: April 2019

AbstractBoth leadership and public value are increasingly seen as concepts highly relevant to public administration, not only because of complex societal challenges but also as ways to address pluralistic interests in society. This article explores in detail the varied conceptualizations of public value and of public leadership. Furthermore, we argue that political astuteness provides an important conceptual linkage between leadership and public value, enabling actors to read, understand and foster coalitions around diverse and sometimes competing interests. In this introduction to the symposium, we analyse the different conceptualizations of public value, of leadership, and also show how the six articles explicitly or implicitly draw on the linking concept of political astuteness. The article assesses how the six articles of the symposium contribute to each of these three concepts.
Changing healthcare provider and parent behaviors in the pediatric post‐anesthesia‐care‐unit to reduce child pain: Nurse and parent training in postoperative stress
Authors: Jenkins Brooke N. , Fortier Michelle A. , Stevenson Robert , Makhlouf Mai , Lim Paulina , Converse Remy , Kain Zeev N. 
Published: April 2019

AbstractBackgroundChildren who undergo surgery experience significant pain in the post anesthesia care unit. Nurse and parent behaviors in the post anesthesia care unit directly impact child postoperative pain. Therefore, we have developed and evaluated (Phase 1) and then tested (Phase 2) the feasibility of a new intervention (Nurse and Parent Training in Postoperative Stress) to alter parent and nurse behaviors in a way consistent with reducing child postoperative pain.MethodsIn Phase 1, a multidisciplinary team of experts (physicians, nurses, and psychologists) developed an empirically‐based intervention which was then evaluated by experienced nurses (N = 8) and parents (N = 9) during focus groups. After revising the intervention based on focus group feedback, it was tested in Phase 2 using a pre‐post study design. Nurses (N = 23) who worked in the recovery room were recruited to be part of both pre‐ and post‐intervention data collection periods. Parents were recruited to be part of either the pre‐ (N = 52) or post‐intervention (N = 60) data collection periods. Nurses and parent‐child dyads were recorded in the post anesthesia care unit and videos were coded for the desired (ie, behaviors that may decrease child pain) and non‐desired (ie, behaviors that may increase child pain) behaviors. Pain data was collected from the children's medical records to assess pain after surgery. The intervention was given to the nurses and parents in the post‐intervention data collection period.ResultsNurses significantly increased their rate of desired behaviors by 231% (P = 0.001; Somer's D = 1) and significantly decreased their rate of non‐desired behaviors by 62% (P = 0.004, Somer's D = −0.88, 95% CI [−1.74, −0.03]). Parents significantly increased their rate of desired behaviors by 124% (P = 0.033). Moreover, the intervention significantly decreased child pain in the post anesthesia care unit (b = −2.19, SE = 0.63, z = −3.46, P = 0.001, 95%CI [−3.43, −0.95]).ConclusionThe intervention was effective in changing nurse and parent behaviors as well as child pain after surgery.
Successful lung donation at the age of 6 weeks: Challenges and lessons learned
Authors: Pavlushkov Evgeny , Muthialu Nagarajan , Spencer Helen , Ellis Clair , Davies Ben , Claydon Sarah , Berman Marius 
Published: April 2019

AbstractA clinical case of successful procurement and transplantation of bilateral lungs from 6‐week‐old infant with sepsis secondary to bacterial meningitis is reported. Forty‐one‐day‐old male infant (height 60 cm, weight 4 kg) died of cerebral edema secondary to Escherichia coli meningitis and bacteremia. Preretrieval assessment included the following: arterial gases; pO2 50.4 kPa (378 mm Hg), pCO2 4.9 kPa (37 mm Hg), on FiO2 100%, PEEP 5 cm H2O. Fiberoptic bronchoscopy showed no secretions nor mucosal inflammation; CXR revealed clear lung fields and pleural spaces. Inspection revealed dense adhesions in pericardial cavity and purulent left hemithorax effusion (urgent Gram‐stain came back as negative) but there was no consolidation in the lung. Good compliance of the lungs on inflation/deflation test was observed. The lungs were retrieved using the technique described. The recipient was a 4‐month‐old infant with alveolar capillary dysplasia and malaligned pulmonary veins. Implantation of the lungs was performed via bilateral thoracosternotomy on cardiopulmonary bypass, cooling to 30°C. Elective support with nitric oxide was used postoperatively. Two years after the transplantation, the recipient doing well with normal lung function. Lung procurement from a 6‐week donor with infectious complications and prolonged ventilation is a challenging undertaking but can be successful and should be attempted whenever possible given the paucity of organs available for pediatric recipients.
Automatic EPAP intelligent volume‐assured pressure support is effective in patients with chronic respiratory failure: A randomized trial
Authors: Orr Jeremy E. , Coleman John , Criner Gerard J. , Sundar Krishna M. , Tsai Sheila C. , Benjafield Adam V. , Crocker Maureen E. , Willes Leslee , Malhotra Atul , Owens Robert L. , Wolfe Lisa F. 
Published: April 2019

ABSTRACTBackground and objectivePatients with chronic respiratory failure are increasingly managed with domiciliary non‐invasive ventilation (NIV). There may be limited ability to provide NIV titration for these complex patients, and ventilatory requirements and upper airway support needs may change over time. Therefore, an automatically adjusting expiratory positive airway pressure (AutoEPAP) algorithm may offer advantages over manually adjusted EPAP for treating these patients. This study compared 4% oxygen desaturation index (ODI4%) values during the use of an AutoEPAP algorithm versus manual EPAP titration with the intelligent volume‐assured pressure support (iVAPS) algorithm.MethodsThis prospective, single‐blind, randomized, crossover study was conducted at six US sites. Patients with chronic respiratory failure (neuromuscular disease, chronic obstructive pulmonary disease, obesity hypoventilation and other aetiologies) and an apnoea–hypopnoea index of >5/h who were already established NIV users underwent a single night of NIV with the iVAPS manual EPAP and iVAPS AutoEPAP in the sleep laboratory in random order.ResultsA total of 38 patients constituted the study population. Mean ODI4% was statistically non‐inferior with AutoEPAP versus manual EPAP (P < 0.0001). There was no difference in the effect on ODI4% across respiratory failure subgroups. Ventilation parameters and gas exchange were similar with either NIV mode, indicating equally effective treatment of respiratory failure. Sleep parameters were improved during AutoEPAP versus manual EPAP.ConclusionA single night of NIV using the iVAPS with AutoEPAP algorithm was non‐inferior to a single night of iVAPS with manual EPAP titration in patients with respiratory failure.Clinical Trial Registration: NCT02683772 at clinicaltrials.gov
Three‐fold nature of coastal progradation during the Holocene eustatic highstand, Po Plain, Italy – close correspondence of stratal character with distribution patterns
Authors: Amorosi Alessandro , Barbieri Giulia , Bruno Luigi , Campo Bruno , Drexler Tina M. , Hong Wan , Rossi Veronica , Sammartino Irene , Scarponi Daniele , Vaiani Stefano Claudio , Bohacs Kevin M. 
Published: April 2019

AbstractAlthough general trends in transgressive to highstand sedimentary evolution of river‐mouth coastlines are well‐known, the details of the turnaround from retrogradational (typically estuarine) to aggradational–progradational (typically coastal/deltaic) stacking patterns are not fully resolved. This paper examines the middle to late Holocene eustatic highstand succession of the Po Delta: its stratigraphic architecture records a complex pattern of delta outbuilding and coastal progradation that followed eustatic stabilization, since around 7·7 cal kyr bp. Sedimentological, palaeoecological (benthic foraminifera, ostracods and molluscs) and compositional criteria were used to characterize depositional conditions and sediment‐dispersal pathways within a radiocarbon‐dated chronological framework. A three‐stage progradation history was reconstructed. First, as soon as eustasy stabilized (7·7 to 7·0 cal kyr bp), rapid bay‐head delta progradation (ca5 m year−1), fed mostly by the Po River, took place in a mixed, freshwater and brackish estuarine environment. Second, a dominantly aggradational parasequence set of beach‐barrier deposits in the lower highstand systems tract (7·0 to 2·0 cal kyr bp) records the development of a shallow, wave‐dominated coastal system fed alongshore, with elongated, modestly crescent beaches (ca2·5 m year−1). Third, in the last 2000 years, the development of faster accreting and more rapidly prograding (up toca15 m year−1) Po delta lobes occurred into 30 m deep waters (upper highstand systems tract). This study documents the close correspondence of sediment character with stratal distribution patterns within the highstand systems tract. Remarkable changes in sediment characteristics, palaeoenvironments and direction of sediment transport occur across a surface named the ‘A–P surface’. This surface demarcates a major shift from dominantly aggradational (lower highstand systems tract) to fully progradational (upper highstand systems tract) parasequence stacking. In the Po system, this surface also reflects evolution from a wave‐dominated to river‐dominated deltaic system. Identifying the A–P surface through detailed palaeoecological and compositional data can help guide interpretation of highstand systems tracts in the rock record, especially where facies assemblages and their characteristic geometries are difficult to discern from physical sedimentary structures alone.