bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artificial neural network risk prediction of chronic obstructive pulmonary disease (COPD) exacerbations using urine biomarkers
Authors: Yousuf Ahmed J. , Parekh Gita , Farrow Malcolm , Ball Graham , Graziadio Sara , Wilson Kevin , Lendrem Clare , Carr Liesl , Watson Lynne , Parker Sarah , Finch Joanne , Glover Sarah , Mistry Vijay , Porter Kate , Duvoix Annelyse , O'Brien Linda , Rees Sarah , Lewis Keir E. , Davis Paul , Brightling Christopher E. 
Published: November 2024

BackgroundChronic obstructive pulmonary disease (COPD) exacerbations cause considerable morbidity and mortality. We sought to identify a panel of urine biomarkers that can distinguish between stable and exacerbation states and predict risk of future exacerbations.MethodsA retrospective discovery study was done measuring 35 biomarkers implicated in COPD pathogenesis in paired urine samples from 55 COPD subjects during stable and exacerbation states. A logistic regression model combining the 10 most discriminatory biomarkers in distinguishing between stable and exacerbation states was developed as a near-patient dipstick test with an opto-electronic reader. This biomarker panel was tested in a prospective study of 105 COPD subjects who undertook daily home urine testing over 6 months. The regression model was validated in paired samples from 26 individuals out of 105. An artificial neural network (ANN) using the urine biomarkers from 85 out of 105 subjects was developed and tested as a clinical decision tool to predict risk of an exacerbation.ResultsThe 10-biomarker panel (NGAL, TIMP1, CRP, fibrinogen, CC16, fMLP, TIMP2, A1AT, B2M, and MMP8) was able to distinguish exacerbationversusstable state in the discovery study (ROC with an AUC 0.84 [95% CI, 0.76 to 0.92; p <0.01]), and validation study (AUC 0.81 [95% CI; 0.70 to 0.92, p<0.01]). The ANN model predicted an exacerbation within a 13-day window frame with an AUC 0.89 (95% CI 0.89–0.90) and identified an exacerbation median (interquartile range) 7 (5 to 9) days prior to clinical diagnosis.ConclusionWe identified a panel of biomarkers that can distinguish between stable and exacerbation state and using an artificial neural network model, it can predict exacerbations before symptoms occur.
A double knockout for zinc transporter 8 and somatostatin in mice reveals their distinct roles in regulation of insulin secretion and obesity
Authors: Yang Zhongyue , Kirschke Catherine P. , Cai Yimeng , Huang Liping 
Published: November 2024

Abstract
Background
Both zinc transporter 8 (ZnT8) and somatostatin (Sst) play crucial roles in the regulation of insulin and glucagon secretion. However, the interaction between them in controlling glucose metabolism was not well understood. The aim of this study was to explore the interactive effects of a double knockout of Znt8 and Sst on insulin and glucose metabolism in mice.

Methods
Co-expression of ZnT8 with hormones secreted from gastrointestinal endocrine cells of mice was determined using immunofluorescence. Male Znt8 knockout (Znt8KO), Sst knockout (SstKO), double knockout for Sst and Znt8 (DKO), and the wild-type (WT) mice were fed a regular chow diet (CD) or a high-fat diet (HFD) at 3 weeks old for 15 weeks. Weights and fasting or fed glucose levels were determined. Glucose and insulin tolerance tests were performed; metabolic-relevant hormone levels including insulin, glucagon, glucagon-like peptide 1, Pyy, and leptin were determined.

Results
ZnT8 is co-expressed with Sst in a subpopulation of endocrine D cells in the gastrointestinal tract. The absence of ZnT8 expression resulted in an increased density of the dense cores in the secretory granules of the D cell. DKO mice had reduced weight compared to WT when maintained on the CD. Compared to Znt8KO and SstKO, DKO mice did not show significant differences in fed or fasting blood glucose level regardless of dietary conditions. However, the CD-fed DKO mice had impaired insulin secretion without alterations in islet morphology or numbers. Moreover, DKO mice displayed diet-induced insulin resistance and disrupted secretion of metabolic-related hormones.

Conclusions
Somatostatin as well as a normal insulin sensitivity are required for normalizing glucose metabolism in Znt8KO mice. ZnT8 may play a role in regulating fat mass and leptin secretion. These findings shed light on the multifaceted nature of Znt8 and Sst interactions, opening new avenues to understand their roles in controlling glucose metabolism and fat mass.
Comparison of heart rate variability, hemodynamic, metabolic and inflammatory parameters in various phases of decompansatory hemorrhagic shock of normal and vagotomized conscious male rats
Authors: Khodadadi Fateme , Punait Sujata , Ketabchi Farzaneh , Khodabandeh Zahra , Bahaoddini Aminollah , Lewis Gregory F. 
Published: November 2024

Abstract
Background
Heart rate variability (HRV) analysis has shown promise as a valuable complementary tool for clinical assessment in trauma cases. This study aims to evaluate the utility of HRV in monitoring different severities of hemorrhagic shock (HS) and its correlation with traditional hemodynamic and metabolic parameters.

Methods
Male Sprague–Dawley rats were divided into different experimental groups, including those with and without vagotomy, and were exposed to different classes of decompensatory HS. To induce varying severities of HS, volume resuscitation was delayed by gradually returning 0%, 20%, or 50% of the shed blood volume at the end of the compensation phase, referred to as 0% DFR, 20% DFR, and 50% DFR class, respectively. Hemodynamic parameters were monitored, and HRV was calculated. Levels of TNF-α and IL-10 were determined in lung tissue at the end of the experiments. Correlations between HRV, hemodynamic parameters, inflammatory gene expression and arterial blood gas variables were evaluated.

Results
HRV showed increased power of the low-frequency (LF) and respiratory sinus arrhythmia (RSA) in all groups during the hypotension phase of HS (Nadir 1). Subdiaphragmatic vagotomy blunted the increase in the LF component in the Nadir 1. After volume resuscitation, systolic blood pressure (SBP), RSA and LF returned to baseline in the 0% DFR and 20% DFR classes. However, animals in 50% DFR class exhibited a reduced SBP and LF and lower pH. Notably, strong correlations were found between LF and SBP as well as tissue hypoperfusion markers. The expression of TNF-α in the lung was increased in all HS groups, while this gene expression was significantly higher in the vagotomized animals.

Conclusion
The alterations in HRV components were found to be significantly correlated with the hemodynamic and metabolic status of the animals, while showing no association with inflammatory responses. Additionally, the intervention of subdiaphragmatic vagotomy significantly impacted both HRV components and inflammatory responses. Collectively, these findings suggest the potential of HRV components for the assessment of the presence and severity of HS.
Family events and child behavior in late childhood: a cross-sectional study
Authors: Hosokawa Rikuya , Tomozawa Riho , Fujimoto Megumi , Katsura Toshiki 
Published: November 2024

Abstract
Background
Children’s social behavior develops through diverse experiences. However, the relationship between children’s experiences of celebratory events and their behavioral development has not been previously quantified. Therefore, this study aimed to quantitatively explore this relationship.

Methods
In 2020, a self-reported questionnaire was completed by 653 sixth-grade students (aged 11–12 years) and/or their caregivers in Nagoya, Aichi, a major metropolitan area in Japan. The main items surveyed were children’s experiences with events celebrated by their family and their behavioral development. The Strengths and Difficulties Questionnaire was used to assess children’s behavior. This questionnaire identifies behavioral and emotional problems and prosocial behavior. Multiple regression analysis was conducted with the number of family events experienced by children as the explanatory variable and their emotional and prosocial behavior as the objective variables.

Results
Children from families who participated in fewer family events tended to have higher problematic behavior risk scores and lower prosociality scores compared with their counterparts. Compared with children who participated in more than 20 family events, those who participated in fewer than 10 family events had more than three times the risk for exhibiting problem behaviors (odds: 3.558, 95% CI: 1.252–10.111, p = .017) and prosocial problems (odds: 3.184, 95% CI: 1.726–5.876, p < .001). Conversely, children from families who participated in more family events tended to have lower problematic behavior risk scores and higher prosociality scores.

Conclusions
A higher number of family events may reduce the risk of behavioral problems in children and increase their prosociality. Enjoying family events may be beneficial for social adjustment.
Improvement of persistent impairments in executive functions and attention following electroconvulsive therapy in a case control longitudinal follow up study
Authors: Hammar Åsa , Ronold Eivind Haga , Spurkeland Malene Alden , Ueland Rita , Kessler Ute , Oedegaard Ketil J. , Oltedal Leif 
Published: November 2024

Abstract
Background
How cognition is influenced by electroconvulsive treatment (ECT) and major depressive disorder (MDD) is still debated. The development and etiology of neurocognitive impairment in MDD were examined by investigating the cognitive profile following ECT related to the state, scar, and trait perspectives, with the former predicting improvements parallel with depressive symptoms, while the two latter expected persisting impairments. Executive functions (EF) and attention are central to cognition and alterations in these functions could influence other domains like memory. The main aims of the present study were to examine the short and long-term effects of ECT on EF and attention in patients with major depressive disorder by exploiting the rapid antidepressant effect of this treatment.

Methods
A case-control longitudinal follow-up design was used to investigate the effects of unilateral brief-pulse ECT on EF and attention in patients with depression (n = 36) compared to untreated healthy controls (n = 16). EF and attention were measured pre-treatment, approximately two weeks, and six months post-treatment.

Results
The patient group showed significantly worse performance on most tests compared to healthy controls pre-treatment, and no short- or long-term worsening of EF and attention following ECT was found. Significant improvement was identified in patients’ attention, processing speed and inhibition after ECT.

Conclusions
The present study showed that there was no cognitive worsening after ECT treatment. An improvement in several of the tests measuring inhibition, attention, and processing speed was parallel to symptom reduction, with the former showing associations to symptom change, suggesting state-related effects from improved mood. Still, the patient group performed significantly worse on most measures both pre-treatment and at the short and long-term follow-ups, indicating prevailing trait or scar effects on cognitive functions and potential lack of practice effects.

Clinical trial number
NCT04348825 (14.04.20).
Parenteral prostacyclin utilization in patients with pulmonary arterial hypertension in the intermediate-risk strata: a retrospective chart review and cross-sectional survey
Authors: Vaidya Anjali , Sketch Margaret R. , Broderick Meredith , Shlobin Oksana A. 
Published: November 2024

Abstract
Background
Current clinical guidelines support use of parenteral prostacyclin therapy for patients with pulmonary arterial hypertension (PAH) at intermediate risk. The objective of this study was to assess parenteral prostacyclin therapy use among patients at intermediate risk according to the Comparative, Prospective Registry of Newly Initiated Therapies for Pulmonary Hypertension (COMPERA) 2.0 four-strata risk assessment model.

Methods
This was a retrospective chart review and cross-sectional online survey of healthcare professionals (HCPs). Included patients were classified as intermediate-low or intermediate-high risk per COMPERA 2.0 between 2016 and 2020 (index visit), initiated on a parenteral prostacyclin any time following intermediate risk assessment, and had World Health Organization (WHO) Functional Class (FC), 6-minute walk distance (6MWD), and B-type natriuretic peptide/N-terminal pro B-type natriuretic peptide (BNP/NT-proBNP) assessments at index and first comprehensive follow-up visits (follow-up).

Results
A total of 139 HCPs (53% community-based, 47% Pulmonary Hypertension Care Center-based) participated in the survey and provided 350 patient records; among these, mean age (SD) was 54.1 (15.3) years and 52% were female. Median (IQR) time from parenteral prostacyclin initiation to follow-up was 3.0 months (2.0, 7.0). At parenteral prostacyclin initiation for the 280 patient records with available COMPERA 2.0 assessments, 62% of patients were intermediate-high risk, 33% were intermediate-low risk and 3% were low risk, improving to 38%, 53%, and 8%, respectively, at follow-up.

Conclusions
Improvements were seen for the individual COMPERA 2.0 risk calculator parameters and for several other clinical parameters. Findings from this study substantiate recent guidelines suggesting earlier use of this treatment in intermediate-risk patients with PAH.

Clinical trial number
Not applicable.
Global collaborative research in metabolic and bariatric surgery (GCRMBS): current status and directions for the future
Authors: Pouwels Sjaak , Thaher Omar , Vladimirov Miljana , Felsenreich Daniel Moritz , Pascotto Beniamino , Taha Safwan , Bausch Dirk , Oviedo Rodolfo J. 
Published: November 2024

AbstractObesity has been recognized as a chronic disorder by the World Health Organisation (WHO) and was first reported in the Paleolithic age. In the recent years there has not been an international collaborative that facilitates professional cooperation on a worldwide level to increase the output of high-level evidence in the fields of obesity treatment and metabolic and bariatric surgery (MBS). In other surgical and medical fields, international collaborative research networks have shown to increase the quality and amount of treatment-changing evidence. In general, Global Collaborative Research in MBS (GCRMBS) should have the following goals: (1) clinical specialty–based research in obesity and MBS, (2) designing research protocols and studies to generate long-term data in obesity and MBS, (3) understanding the uncommon/rare complications and events associated with obesity and MBS, (4) increasing the number of participants in research and (5) investigating ethical and racial disparities in bariatric research. This review gives an overview of the current status and the future of international collaborative research in MBS.
Eurasian spruce bark beetle detects lanierone using a highly expressed specialist odorant receptor, present in several functional sensillum types
Authors: Yuvaraj Jothi Kumar , Kandasamy Dineshkumar , Roberts Rebecca E. , Hansson Bill S. , Gershenzon Jonathan , Andersson Martin N. 
Published: November 2024

Abstract

Background
Insects detect odours using odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs) in the antennae. Ecologically important odours are often detected by selective and abundant OSNs; hence, ORs with high antennal expression. However, little is known about the function of highly expressed ORs in beetles, since few ORs have been functionally characterized. Here, we functionally characterized the most highly expressed OR (ItypOR36) in the bark beetle Ips typographus L. (Coleoptera, Curculionidae, Scolytinae), a major pest of spruce. We hypothesized that this OR would detect a compound important to beetle fitness, such as a pheromone component. We next investigated the antennal distribution of this OR using single sensillum recordings (SSR) and in situ hybridization, followed by field- and laboratory experiments to evaluate the behavioural effects of the discovered ligand.

Results
We expressed ItypOR36 in HEK293 cells and challenged it with 64 ecologically relevant odours. The OR responded exclusively to the monoterpene-derived ketone lanierone with high sensitivity. Lanierone is used in chemical communication in North American Ips species, but it has never been shown to be produced by I. typographus, nor has it been studied in relation to this species’ sensory physiology. Single sensillum recordings revealed a novel and abundant lanierone-responsive OSN class with the same specific response as ItypOR36. Strikingly, these OSNs were co-localized in sensilla together with seven different previously described OSN classes. Field experiments revealed that low release rates of lanierone inhibited beetle attraction to traps baited with aggregation pheromone, with strongest effects on males. Female beetles were attracted to lanierone in laboratory walking bioassays.

Conclusions
Our study highlights the importance of the so-called ‘reverse chemical ecology’ approach to identify novel semiochemicals for ecologically important insect species. Our discovery of the co-localization pattern involving the lanierone OSN class suggests organizational differences in the peripheral olfactory sense between insect orders. Our behavioural experiments show that lanierone elicits different responses in the two sexes, which also depend on whether beetles are walking in the laboratory or flying in the field. Unravelling the source of lanierone in the natural environment of I. typographus is required to understand these context-dependent behaviours.
Layers of inequality: gender, medicalisation and obstetric violence in Ghana
Authors: Yalley Abena Asefuaba 
Published: November 2024

Abstract
Background
This study explored how gender inequalities in health systems influence women’s experiences of obstetric violence in Ghana. Obstetric violence is recognised as a major public health concern and human rights violation. In particular, it reduces women’s trust and use of health facilities for childbirth, thereby increasing the risks of maternal and neonatal mortality. In Ghana, obstetric violence is pervasive and normalised; yet, little is known about the gendered dynamics of this phenomenon.

Methodology
A qualitative study was conducted in eight public health facilities in Ghana. Specifically, semi-structured interviews were conducted with 30 midwives who work in the maternity units and 35 women who have utilised the obstetric services of the hospitals for childbirth. The midwives and women were selected using the purposive sampling technique. The transcripts of the interviews were coded using NVivo qualitative data analysis software and were thematically analysed. Secondary materials such as existing data on the medical profession in Ghana were utilised to complement the primary data.

Results
The study revealed that there are huge structural inequalities that keep women at the lower cadres of the health system. Five major themes depicting how gender inequalities contribute to women’s experiences of obstetric violence emerged: gender inequality in the medical profession, unequally and heavily tasked, feminisation of midwifery, patriarchal pressures and ideologies, and gender insensitivity in resource provision. These inequalities impact the kind of care midwives provide, which is often characterised by mistreatment and abuse of women during childbirth. The study also discovered that patriarchal ideologies about women and their bodies lead to power and control in the delivery room and violence has become a major instrument of domination and control.

Conclusion
The hierarchical structure of the healthcare profession puts the midwifery profession in a vulnerable position, with negative consequences for maternity care (obstetric violence). The study recommends that gender-responsive approaches that address structural inequalities in health systems, women’s empowerment over their bodies and male involvement in women’s reproductive care are crucial in dealing with obstetric violence in Ghana.
Resistance intensity test (RIT): a novel bioassay for quantifying the level of acaricide resistance in Rhipicephalus microplus ticks
Authors: Jongejan Frans , Berger Laura , Homminga Laura , Hulsebos Iris , Petersen Alita , Ferreira Priscila Teixeira , Reck José , Klafke Guilherme 
Published: November 2024

Abstract
Background
One bioassay for detecting acaricide resistance in livestock ticks is the adult immersion test (AIT), wherein engorged ticks are briefly immersed into a solution of a particular acaricidal compound and examined for mortality, their egg-laying capacity and offspring hatchability in vitro. Usually, the recommended label dose or an established discriminating dose of an acaricide is used to determine high mortality (≥ 95%) of susceptible tick strains. Such a test intends to detect the presence of resistance in a tick population. However, the adult immersion test does not directly translate the bioassay results to the predicted efficacy in the field. In this paper, we used the AIT as an initial screening bioassay supplemented with the resistance intensity test (RIT), a novel larval-based bioassay, wherein the resistance level can be determined and translated to the expected field efficacy. This was done by adopting World Health Organisation (WHO) guidelines for resistance detection in mosquitoes, which combines a 1 × recommended dose with 5 × and 10 × concentrated doses to reveal low, moderate and high resistance intensity, respectively.

Methods
Engorged Rhipicephalus microplus ticks were collected from cattle at six different ranches across Rio Grande do Sul, Brazil, as part of the state’s acaricide resistance surveillance program. Groups of adult ticks from each field collection were subjected to the AIT from each field sample. Additionally, engorged female ticks from each ranch were allowed to lay eggs, and their larval progeny aged 14 to 28 days were then used in the RIT. Deltamethrin and a combination of cypermethrin, chlorpyrifos and piperonyl butoxide were used in both tests, and the results were statistically analysed.

Results
The in vitro efficacy of deltamethrin against adult ticks in the AIT ranged between 8.74% and 25.38%. The corresponding RIT results on their larval progeny indicated a high resistance level. In the immersion test, the in vitro efficacy of the combination of cypermethrin, chlorpyrifos, and piperonyl butoxide against adult ticks ranged between 49.31% and 100%. The corresponding RIT results on their larval progeny indicated a similar response ranging from fully susceptible to low or moderate resistance. The Pearson correlation coefficient (r = 0.883) showed a high correlation between tick mortality at the 1 × recommended concentrations of acaricides in both tests.

Conclusions
The resistance intensity test is a valuable addition to the range of bioassays currently available for detecting acaricide resistance by determining the level of acaricide resistance. This is relevant to whether or not to continue using a particular acaricidal class for controlling cattle ticks.

Graphical Abstract