bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping and validation of a major QTL, QTkw.cau-3DL, controls kernel weight by regulating kernel length in wheat
Authors: He Xi , Liu Jilu , Ren Xiaomeng , Wei Shurong , Zhu Zhenzhen , Zhang Fuping , Hu Sijia , Ding Yanpeng , Sun Fangyao , Han Dong , Bai Guihua , Ni Zhongfu , Sun Qixin , Su Zhenqi 
Published: November 2024

Abstract

Increasing kernel weight is an effective way for improving grain-yield in wheat. Identification of major QTLs for kernel weight without negative effects on other yield-related traits is important to continuous yield improvement. We developed a population of F6 recombinant inbred lines (RILs) from Jimai120 x Jimai325 and identified eight QTLs for thousand-kernel weight (TKW), kernel length (KL) and kernel width (KW) in five environments. One QTL on chromosome arm 3DL consistently showed major effects on TKW (QTkw.cau-3DL) and KL in five field experiments, accounting for up to 16.43% and 13.87% of phenotypic variation, respectively. QTkw.cau-3DL was confined to a 5.72 Mb (3.48 cM) interval between 554.39 and 560.11 Mb. This QTL was validated in a pair of near-isogenic lines (NILs) and in a new double haploid (DH) population. QTkw.cau-3DL increased kernel weight per spike (KWPS) without any negative effect on heading data (HD), plant height (PH), spike length (SL), spikelet number per spike (SNS) and kernels number per spike (KNPS). It increased 6.2% of grain yield under regular field production conditions. Analyses on its marker haplotype and geographical distribution in a nationwide collection of 630 Chinese wheat cultivars and breeding lines showed that QTkw.cau-3DL has not been widely deployed in Chinese wheat breeding programs. QTkw.cau-3DL is a novel QTL for increasing TKW through increasing KL, therefore, it is an important locus for enhancing wheat grain yield. The tightly linked user-friendly markers developed in this study should facilitate map-based cloning and marker-assisted selection of the QTL in wheat breeding programs.
Embryonic Transfer Post-Vaginal Bleeding: Analysis of the Necessity of Tuberculosis Screening
Authors: Kexin Liu , Weina Gao , Xianlan Zhao 
Published: November 2024

Abstract

Objective: To analyze the clinical manifestations and outcomes of 10 cases of post-transplant tuberculosis and to explore the necessity of screening for tuberculosis in patients with vaginal bleeding after in-vitro fertilization-embryo transfer (IVF-ET).
Methods: A retrospective analysis was conducted on 10 clinical cases of active tuberculosis during pregnancy following IVF-ET treated at the First Affiliated Hospital of Zhengzhou University from January 2018 to October 2024. The relationship between vaginal bleeding and active tuberculosis was assessed, and the potential value of tuberculosis screening in such patients was discussed.
Results: (1) General situation: Among the 10 pregnant patients with confirmed active tuberculosis after IVF-ET, 3 had a history of tuberculosis before pregnancy (3/10), 6 were diagnosed with tuberculosis for the first time during pregnancy (6/10), and 1 was diagnosed with tuberculosis after termination of pregnancy (1/10); all but one were primiparous (9/10). (2) Clinical symptoms: Among the 10 patients, fever of varying degrees was the main symptom in 9 cases (9/10), accompanied by vaginal bleeding in 8 cases (8/10), with the lung being the main affected organ, and the most common types of pulmonary tuberculosis were acute miliary pulmonary tuberculosis and hematogenous disseminated pulmonary tuberculosis. (3) Maternal and fetal outcomes: It took about 2 weeks from the onset of tuberculosis to diagnosis, and only 4 patients (4/10) had viable fetuses; all live birth cases were terminated by cesarean section, with 1 term live birth and 3 newborns born prematurely and transferred to the neonatal department.
Conclusion: Pregnancy outcomes in active tuberculosis after embryo transfer are poor. Screening for tuberculosis in patients with vaginal bleeding after IVF-ET is necessary to some extent. Clinically, it is necessary to be vigilant about the possibility of tuberculosis onset in patients after IVF-ET who are preventing miscarriage. Early diagnosis and regular anti-tuberculosis treatment can improve clinical prognosis.
Community perspectives regarding preparedness, response and prevention of Ebola Virus Disease amidst COVID-19 pandemic, an exploratory descriptive qualitative study using a community engagement approach in Mbale City
Authors: Kagoya Enid Kawala , Nsubuga Allan G. , Ewing Helen , Atuhairwe Irene , Ijangolet Esther , Otim Eric , Ronald Kibuuka , Jesca Atugonza , Okibure Ambrose , Okello Francis , Nakattudde Prossy , Nyangoma Faith , Namara Racheal , Nakabaale Mark , Nakawuka Betty , Nakawuki Ashley , Kizito Prisca , Waako Paul , Wandabwa Julius , Makoko Tonny Brian , Musaba Milton , Ahaisibwe Bonaventure , Barugahare Banson 
Published: November 2024

Abstract

Background: Ebola virus disease (EVD) is a rare and deadly infectious hemorrhagic fever disease. Four of the 6 known Ebola virus species can cause human disease. Since 1976, EVD outbreaks have been recorded sporadically in East, Central, and West Africa. Uganda has experienced 6 documented outbreaks of EVD since 2000 that can be attributed to 3 species of Ebola viruses (Zaire, Sudan, and Bundibugyo). Despite substantial advances in clinical management, the case fatality ratio for EVD remains high, reaching more than 60% for the Zaire Ebola virus in 2018–2020.
Methods. The study followed a descriptive qualitative study design. Data were collected using an interviewer-administered questionnaire with both closed and open-ended questions. Data were analysed using ATLAS Pro.
Results: Our results illustrate the lack of health education in the community among the different categories of people and this comes from an emic perspective. We identified 4 high-level themes and 19 subthemes, (1) Knowledge of Ebola Virus Disease, (2) Prevention of EVD in the community, (3) Preparedness for Ebola Virus Disease, and (4) Community response to Ebola Virus Disease and the final thematic area (4) which included giving first aid, using traditional medicines, and approaching a health facility among others.
Conclusions: With all the available information about the previous outbreaks in Uganda, it is surprising that less effort was made this time round to take local sensibilities and culture into account among other social factors contributing to the poor practices of masses toward the EVD, preparedness, prevention, and response.
Enhanced Automated Identification of Human Activity Using an Adaptive Framework
Authors: Bansal Neha , Bansal Atul , Gupta Manish 
Published: November 2024

Abstract

The incorporation of human-computer interface technologies into daily life has garnered the interest of researchers in developing more advanced autonomous systems. Human-computer interaction systems can succeed in actual applications by addressing the shortcomings of existing techniques. This study focuses on a crucial application of human-computer interaction known as human activity recognition. Human Activity Recognition (HAR) pertains to the identification and classification of human gestures, activities, and many forms of interactions. The system is taught to recognize human activities using video-based data streams and photos as input. The sensor data use any available touch or touchless sensing devices. Gyroscopes, Bluetooth, sound sensors, accelerometers, and other integrated sensors found in contemporary sophisticated smartphones exemplify sensing technologies. This research combines video-based and sensor-based activity recognition. Four models are proposed for the HAR system, comprising two sensor-based models and two video-based models. The utilized models are the Machine Learning-based Voting Classifiers for Human Activity Recognition (MLVC) and the Ensemble Convolutional Neural Networks. The suggested models are assessed against the prevailing leading tactics utilizing the metric of recognition accuracy.
Car Safety System With Airbag Notification
Authors: Student VENUSREE. T , Student Tanushree RS , Student Praveen j 
Published: November 2024

Abstract

Every year the lives of approximately 1.3 million people are cut short because of a road traffic crash. Between 20 and 50 million more people suffer non-fatal injuries, with many incurring a disability because of their injury. The first feature detects whether the driver of the car is feeling drowsy while driving or not. This is done using a camera which is controlled by the Raspberry Pi. If the driver is drowsy the system alerts using speakers that calls out a unique line and indicates by a sound which can be useful in alerting other passengers in the car about the Drivers Drowsiness. The second feature includes a vibration sensor which can detect any jerks that the car may receive after its impact or accident. The vibration sensing is paired with a GSM alert notification to the hospital and a police station whose number will be registered at the start of the system. The system aims to demonstrate a safety system for driver of the car. It has these features which will either alert or notify the concerned party about unfortunate accidents happened to the car and its passengers.
Leeuwenhoekiella obamensis sp. nov., a novel marine bacterium isolated from the surface water of a Japanese fishing port
Authors: Machii Yuka , Tsukamoto Mao , Kataoka Takafumi , Kondo Ryuji 
Published: November 2024

Abstract

A novel aerobic marine bacterium, FRT2T, was isolated from the surface water of a fishing port in Fukui, Japan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain FRT2T clustered with the genus Leeuwenhoekiella. The closest relatives of the strain FRT2T were Leeuwenhoekiella palythoae KMM 6264T and Leeuwenhoekiella nanhaiensis G18T with 16S rRNA gene sequence identities of 95.1% and 94.5%, respectively, suggesting that FRT2T is a novel species of the genus Leeuwenhoekiella. The values of digital DNA-DNA hybridisation and average nucleotide identity between FRT2T strain and type strains of species of the genus Leeuwenhoekiella were lower than the threshold for species delineation, indicating that FRT2T is a novel species of the genus Leeuwenhoekiella. Cells were Gram-negative, strictly aerobic, yellow-orange-pigmented, motile by gliding, and had a rod shape with 0.3–0.8 µm width and 1.6–4.1 µm length. Saline was needed to grow FRT2T with optimal growth in the presence of 3.0–4.0% (w/v) NaCl. Growth was observed from pH 5.5 to 8.5 (optimum pH 6.5–7.5) and at temperatures between 10 and 39°C (optimum 25–35°C). Major cellular fatty acids were iso-C15:0, iso-C15:1, iso-C17:0 3-OH and summed feature 3 (C16:1ω6c and/or C16:1ω7c). The only respiratory quinone was MK-6. The DNA G + C content of FRT2T was 38.9 mol%. Based on its genetic and phenotypic features, the strain FRT2T represents a novel species, for which the name Leeuwenhoekiella obamensis sp. nov. is proposed, with the type strain FRT2T (= BCRC 81451T = JCM 36940T). The discovery of this novel bacterial species provides the scope for future research.
Microfacies, Depositional Settings, Diagenesis and Diagenetic Evolution of the Ordovician to Devonian Homoclinal Ramp-Type Carbonate Platform Setul Group, Northern Peninsular Malaysia
Authors: Radzir Nurul Afifah Mohd , Samah Mohammad Ezanie Abu , Ali Che Aziz , Mohamed Kamal Roslan 
Published: November 2024

Abstract

The Ordovician to Devonian strata of the Setul Group in the northern region of Peninsular Malaysia represent medium- to thick-bedded, white to grayish limestone rich in biota components. A detailed study of microfacies analysis and diagenetic investigation revealed eleven (11) microfacies and five (5) diagenetic histories. The recognized microfacies include 2 mudstone, 2 wackestone, 2 packestone, 3 grainstone, chert and carbonaceous shale. The microfacies and their associations represent a homoclinal ramp depositional setting designated on the basis of the rock components. The Setul Group has undergone significant diagenetic alteration due to various diagenetic events that have altered the primary and secondary porosities. The recognized diagenetic alterations include micritization, cementation, neomorphism, compaction and dolomitization. The paragenetic sequence reveals that the carbonate rock is modified in phreatic zones and involves early to late diagenetic evolution. The microfacies and diagenetic alteration revealed that the Setul Group was deposited in a shallow marine to deep ocean environment dominated by siliciclastic and carbonate sediments.
Latent class analysis of academic adjustment and mental health among Brazilian college students: association with depression and suicide ideation
Authors: Altavini Camila Siebert , Santana Geilson Lima , Andrade Laura Helena , Oliveira Lúcio Garcia , Andrade Arthur Guerra , Gorenstein Clarice , Wang Yuan-Pang 
Published: November 2024

Abstract

Purpose
Suicide is a leading cause of death among 15-29-year-olds. Effective prevention strategies are urgent, particularly for university students, where knowledge gaps regarding suicide-related factors hinders preventative efforts. The present study aimed to identify subgroups within Brazilian college students to examine the relationship of identified subgroups with suicidal ideation (SI) and depression.
Methods
Using academic and mental health indicator from a national survey of Brazilian college students, a latent class analysis was conducted to identify subgroups of students based on similar characteristics. Meaningful classes were subjected to logistic regression to identify potential associations with SI and depressive symptoms.
Results
Four distinct classes were identified, labeled as: “ordinary”, “psychologically distressed”, “dissatisfied”, and “binge drinkers”. The subgroups experiencing psychological distress and dissatisfaction were associated with a higher likelihood of presenting SI and depressive symptoms.
Conclusion
The impact of academic life on students' mental health must be closely monitored by the universities’ pedagogical and health services. Early identification of students in psychological distress is essential for appropriate referral to supportive services. Assessment of the relationship between suicide-related vulnerabilities is still very necessary to develop adequate prevention plans in educational settings.
Probiotic-Derived Exopolysaccharide as a Natural Stabilizer for Limonene Emulsions: A Novel Approach to Enhancing Stability and Bioactive Properties
Authors: Balyan Sangeeta , Dhowlaghar Nitin , Dadwal Vikas , Bhattarai Samikshya , Jha Deepak Kumar , Patil Bhimanagouda S. 
Published: November 2024

Abstract

Lactobacillus-derived (LAB) exopolysaccharide (EPS) was used as an emulsifier to encapsulate limonene enantiomers, including S- (SL) and D- (DL). EPS and emulsions were analyzed for physiochemical and rheological behavior, followed by emulsifying stability and antimicrobial activity against food-borne pathogens. EPS was extracted and yielded 1.1 g/L amount with an optimized supplementation of 5 % carbon source at pH 6.1 and characterized using fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermo-gravimetric analysis (TGA), along with physiochemical properties. At 2 % EPS in 60/40 O/W emulsion, a desired mean particle size and polydispersity index (PDI) were acquired by comparing different O/W ratios. Optimized emulsions showed higher stability under different physiological parameters: temperatures (-20 to 60 ºC), pH (2-9), and salinity. In FTIR analysis, emulsions showed OH and C=O stretching acquired from EPS and the presence of cyclohexane-1-ene group from encapsulated limonene. Notably, our study is among the first to demonstrate LAB-derived EPS as a natural and effective emulsifier for stabilizing limonene, providing both improved stability and antimicrobial efficacy against E. coli and L. monocytogenes compared to free oils. These findings illustrate that LAB-derived EPS is a promising natural emulsifier for encapsulating volatiles, with significant applications in food safety.
Sonochemistry-assisted sol-gel synthesis of Hydroxyapatite
Authors: Cruz-Ávila Camila , Hernández-Padrón Genoveva , Meneses Víctor Manuel Castaño 
Published: November 2024

Abstract

Sonochemistry was employed to assist the in-situ synthesis hydroxyapatite by sol-gel process aqueous using Ca(NO3)2·4H2O and (NH4)H2PO4 (HApS). The synthesis by assisted sonochemistry reduced the reaction time from 3 h to 11 min. The formation of HApS, hidroxiapatite (HAp) and other compounds during heat treatment at 0, 120, 350 and 700 0C were identified and characterized by FTIR, Raman Dispersive, X-ray and SEM. The spectroscopy characterization showed that chemical bonds in the HApS are the same position that HAp on the main phosphate group peaks appeared at 1098, 1034, 962, 604 and 562 cm− 1 for IR while at 796 cm− 1 for Raman, which was attributed to the asymmetric stretching mode of PO43−. By means of X-ray diffraction, the hexagonal structure of HApS obtained by sonochemistry could be verified. The morphology characterized by SEM showed the similar structure satisfying the porosity and needles of the scaffold bone. This study compared the formation hydroxyapatite with and without sonochemistry.
ANN-based modeling and isotherm-kinetic study of dye adsorption on acid-modified manila tamarind seed biomass
Authors: Karishma S. , Deivayanai V. C. , Thamarai P. , Saravanan A. , Yaashikaa P. R. , Vickram A. S. 
Published: November 2024

Abstract

Plant biomass has been increasingly utilized as an adsorbent material alternate to chemically synthesized activated carbon. Manila tamarind seed has been acid-modified in order to improve the biomass's remediation capabilities. Scanning electron microscopy analysis showed that chemically activated manila seed biomass was rough and porous, with voids present. The ideal circumstances for the eriochrome black and brilliant orange dye removal were found through batch investigations. The optimal values are adsorbent dosage – 3.5 g/L, temperature – 303 K, contact time – 50 min and 40 min, and pH – 6 and 9 for EB and BO dye respectively. Adsorption capacity of AAPDB for EB and BO dye was observed to be 97.12 mg/g and 113.43 mg/g respectively. The best fitting isotherm and kinetic models were Freundlich and Pseudo-first order denoting the multilayer adsorption. The artificial neural network model of both dyes' adsorption was used to link the experimental dye removal values with the anticipated values of the ANN. Better correlation was demonstrated by the neural network model, with values more than 0.94 suggesting that the model is appropriate for the current study. The potential of acid-modified manila tamarind seed biomass in the dye removal process was validated by modelling studies.
Reduction of blue and total water footprints per unit biomass yield of silage maize with grey water footprint input in subsurface drip irrigation
Authors: Cakmakci Talip , Sahin Ustun 
Published: November 2024

ABSTRACT

Reducing blue and total water footprint outputs in irrigated agriculture with greywater footprint input from irrigation with recycled wastewater is an issue that needs to be investigated in protecting freshwater resources by increasing water availability. Therefore, the effect of three different irrigation levels of recycled wastewater and freshwater in the subsurface drip irrigation (SSDI), surface drip irrigation (SDI), and furrow irrigation (FI) methods on the blue, green, grey, and total water footprints per unit yield of silage maize, which is widely produced worldwide and has high water consumption, was investigated with a 2-year field study. The blue and total water footprints per unit fresh and dry biomass yields in the SSDI were 1.20–1.23-fold lower than that in the SDI and 1.69–1.76-fold lower than that in the FI. Full wastewater irrigation provided the lowest blue, green, and total water footprints per unit yield across all methods. Full wastewater irrigation under SSDI provided the lowest total water footprint per unit fresh biomass yield, similar to the 33% deficit irrigation practice with wastewater. It was concluded that full irrigation with recycled wastewater as a greywater resource under SSDI may be the most suitable application for the sustainable management of scarce blue water resources.
Aloperine Attenuates UVB-induced Damage in Skin Fibroblasts via Activating TFE3/Beclin-1-Mediated Autophagy
Authors: Qiu Mingning , Li Jianchang , Zhang Shuai , Liang Jinglan , Wang Xuguang , Liu Jie 
Published: November 2024

Background:
Aloperine (ALO) is an important active ingredient in the traditional Chinese
medicinal plant Sophora alopecuroides L and has a significant autophagy-stimulating effect.
The effect of ALO on cytotoxicity caused by UVB radiation in skin fibroblasts and the potential
mechanism remains unclear.

Objective:
The present study aimed to assess the effect of ALO on UVB-induced damage in skin
fibroblasts and investigate its possible mechanism.

Methods:
Cell viability, cytotoxicity, caspase-Glo 3/7 activity, apoptosis, and protein expression
were measured in UVB-treated skin fibroblasts in the presence or absence of ALO. Autophagy inhibitors
(chloroquine and bafilomycin A1) and TFE3 siRNA transfection were used to elucidate
the potential mechanisms further.

Results:
These data demonstrate that ALO attenuated cell viability inhibition, apoptosis, cytotoxicity,
and alterations in autophagy-related proteins caused by UVB exposure in skin fibroblasts.
ALO stimulates autophagy activation and TFE3 nuclear localization in UVB-treated skin fibroblasts.
Furthermore, treatment with autophagy inhibitors and TFE3 siRNA reversed the effects
of ALO on UVB-treated skin fibroblasts.

Conclusion:
These results suggest that ALO protects skin fibroblasts against UVB-induced cytotoxicity
by stimulating TFE3/Beclin-1-mediated autophagy.
Comparative Analysis of IMT-P8 and LDP12 Cell-Penetrating Peptides in Increasing Immunostimulatory Properties of HIV-1 Nef-MPER-V3 Antigen
Authors: Sadat Seyed Mehdi , Jahedian Shekoufa , Sabaghzadeh Sahar , Larijani Mona Sadat , Bolhassani Azam 
Published: November 2024

Background:
There have been great efforts in vaccine design against HIV-1 since
1981. Various approaches have been investigated, including optimized delivery systems and effective
adjuvants to enhance the efficacy of selective antigen targets. In this study, we evaluated the
efficiency of IMT-P8 and LDP12 cell penetrating peptides in eliciting immune responses against
HIV-1 Nef-MPER-V3 fusion protein as an antigen candidate. Moreover, the potency of HP91 and
HSP27 was compared as an adjuvant in female BALB/c mice through different regimens.

Methods:
For this purpose, the recombinant Nef-MPER-V3, IMT-P8-Nef-MPER-V3 and LDP-Nef-
MPER-V3 proteins were generated on a large scale. After mice immunization with different regimens,
the secretion of antibodies, cytokines and granzyme B was evaluated by ELISA.

Results:
Our results demonstrated that immunized mice receiving the Nef-MPER-V3 linked to
IMT-P8 exhibited significantly higher levels of IgG compared to other groups. The IMT-P8-Nef-
MPER-V3 with the Hp91 group showed the highest level of humoral response, which was significantly
stronger than the LDP12 formulation using the same antigen (LDP-Nef-MPER-V3). Additionally,
the combination of IMT-P8-Nef-MPER-V3 with either Hp91 or Hsp27 resulted in robust
induction of IFN-γ compared to the LDP-Nef-MPER-V3 group. Furthermore, cytotoxic T lymphocyte
(CTL) activation and proliferation assays indicated that IMT-P8 served as a more effective
CPP, particularly when used in conjunction with the Hp91 adjuvant

Conclusion:
Altogether, the data indicated that Nef-MPER-V3 antigen in different formulations
was effective in eliciting immune responses. This fusion protein has the high potency to induce
both immunity arms, specifically when incorporated with IMT-P8, which showed priority to LDP12.
Moreover, HP91 resulted in a greater humoral and cellular immune activation compared to
HSP27. These findings suggest the potential of IMT-P8 as a superior delivery system for enhancing
immune responses in vaccine development.