bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of uteroplacental vascularisation in early first-trimester pregnancy with contrast-enhanced ultrasound and 3D power Doppler angiography: protocol for a prospective, cross-sectional, multicentre and non-randomised open study (“HOPE Study”)
Authors: Bertholdt Charline , Eszto Marie-Laure , Tournier Mathilde , Hossu Gabriela , Mellouki Naoual , Cherifi Aboubaker , Morel Olivier 
Published: September 2019

IntroductionKnowledge about the mechanisms leading to the establishment of uteroplacental vascularisation is inadequate, and some of what has been thought to be known for decades has recently been challenged by showing that the intervillous space, the major area of maternal-fetal exchange, appears to be perfused by maternal blood at as early as 6 weeks of gestation. The vascular flow then seems relatively constant until 13 weeks when it appears to increase suddenly.ObjectivesThe principal objective is to quantify the perfusion of the intervillous space by contrast-enhanced ultrasonography (CEUS) during the first-trimester at three different gestational ages (8, 11 and 13 weeks). The secondary objectives are to: (1) describe the indicators of vascularisation of the placenta (intervillous space) and the myometrium at the three gestational ages, measured by CEUS and three-dimensional power Doppler (3DPD) angiography; (2) compare the diagnostic performance of CEUS and 3DPD for the demonstration and quantification of uteroplacental vascularisation and (3) establish a biological collection of placentas to increase knowledge about placental development and functions during pregnancy.Methods and analysisThis is a prospective, cross-sectional, multicentre and non-randomised open study. We will include 42 women with ongoing pregnancy and divided into three groups of gestational ages (ie, 14 women by per group): 8, 11 and 13 weeks of gestation. 3DPD and then CEUS will be performed and the data about the perfusion kinetics and the 3DPD indices will be calculated and then compared with each other and for each gestational age.Ethics and disseminationThe appropriate French Ethics Committee Est III approved this study and the related consent forms on 5 April 2016, and the competent authority (Agence Nationale de Sécurité du Médicament et des Produits de Santé) authorised the study on 21 June 2016. The results of this study will be published in a peer-reviewed journal and will be presented at relevant conferences.Trial registration numbersClinicalTrials.gov registry (NCT02884297); EudraCT registry (2015-005655-27).
Mental health informed physical activity for first responders and their support partner: a protocol for a stepped-wedge evaluation of an online, codesigned intervention
Authors: McKeon Grace , Steel Zachary , Wells Ruth , Newby Jill M , Hadzi-Pavlovic Dusan , Vancampfort Davy , Rosenbaum Simon 
Published: September 2019

BackgroundFirst responders (police, fire and ambulance officers) are at a significantly increased risk of experiencing poor mental health, including depression and post-traumatic stress disorder. These conditions are associated with high rates of cardiovascular disease, in part due to low levels of physical activity (PA) and high levels of sedentary behaviour. Using a person’s social support system may be an effective solution to help increase PA levels to improve mental and physical health outcomes. We will examine the efficacy of a group-based online intervention in increasing PA in first responders and their support partners, iteratively codesigned with advisors with lived experience of mental illness among first responders.MethodsThis study will recruit a convenience sample of self-identified sedentary first responders and their self-selected support partners to a 10-week PA programme delivered through a private Facebook group. We will deliver education on predetermined topics related to PA and diet and provide participants with an activity tracker (Fitbit). A stepped-wedged design will be applied to compare multiple baselines to intervention and follow-up phases within subjects. Five cohorts of n=20 will be recruited, with each cohort randomised to a different baseline length. Our primary outcome will be psychological distress (Kessler-6). Secondary outcomes include feasibility, self-report and objective PA data (Simple Physical Activity Questionnaire and Fitbit accelerometry), depression and anxiety (Depression Anxiety and Stress Scale-21 items), post-traumatic stress disorder symptoms (PTSD Checklist for DSM-5), quality of life Assessment of Quality of Life-6 dimensions, sleep quality (The Pittsburgh Sleep Quality Index), suicidal ideation (Suicidal Ideation Attributes Scale) and social support for exercise. The mobile data collection platform MetricWire will be used.Ethics and disseminationEthical approval was obtained from the University of New South Wales, Deupty Vice-Chancellor Research, Human Research Ethics Committee on 3 June 2019, HC180561. Findings will be published in peer-reviewed journals and disseminated at national conferences.Trial registration numberACTRN12619000877189.
Health needs of refugee children identified on arrival in reception countries: a systematic review and meta-analysis
Authors: Baauw Albertine , Kist-van Holthe Joana , Slattery Bridget , Heymans Martijn , Chinapaw Mai , van Goudoever Hans 
Published: September 2019

BackgroundThe worldwide number of refugees has considerably increased due to ongoing wars, national instability, political persecution and food insecurity. In Europe, about one-third of all refugees are children, an increasing number of which are travelling alone. There are often no systematic medical health assessments for these refugee children on entry in reception countries despite the fact that they are recognised as an at-risk population due to increased burden of physical and mental health conditions. We aimed to perform a systematic review of the literature to describe the health status of refugee children on entering reception countries.MethodsA systematic search of published literature was conducted using the terms refugee, immigrant or migrant, medical or health, and screening.ResultsOf the 3487 potentially relevant papers, 53 population-based studies were included in this review. This systematic review showed that refugee children exhibit high estimated prevalence rates for anaemia (14%), haemoglobinopathies (4%), chronic hepatitis B (3%), latent tuberculosis infection (11%) and vitamin D deficiency (45%) on entry in reception countries. Approximately one-third of refugee children had intestinal infection. Nutritional problems ranged from wasting and stunting to obesity.ConclusionsRefugee children entering reception countries should receive comprehensive health assessments based on the outcomes of this systematic review, national budgets, cost-effectiveness and personal factors of the refugees. The health assessment should be tailored to individual child health needs depending on preflight, flight and postarrival conditions.A paradigm shift that places focus on child health and development will help this vulnerable group of children integrate into their new environments.PROSPEROregistrationnumber122561.
Interpregnancy intervals and women’s knowledge of the ideal timing between birth and conception
Authors: Yang Jenny M , Cheney Kate , Taylor Rebecca , Black Kirsten 
Published: September 2019

BackgroundShort interpregnancy intervals (IPIs) are associated with adverse obstetric outcomes. However, few studies have explored women’s understanding of ideal IPIs or investigated knowledge of the consequences of short IPIs.MethodsWe performed a prospective questionnaire-based study at two hospitals in Sydney, Australia. We recruited women attending antenatal clinics and collected demographic data, actual IPI, ideal IPI, contraceptive use, and education provided on birth-spacing and contraception following a previous live birth. We explored associations between an IPI <12 months and a selection of demographic and health variables.ResultsData were collected from 467 women, of whom 344 were pregnant following a live birth. Overall, 72 (20.9%) women had an IPI <12 months only 7.5% of whom believed this was ideal, and the remaining stating their ideal IPI was over 12 months (52.3%) or they had no ideal IPI (40.3%). IPI <12 months following a live birth was significantly associated with younger age (p=0.043) but not with ethnicity, relationship status, education, religion, parity nor previous mode of delivery. IPI <12 months was associated with non-use of long-acting reversible contraception (LARC) (p<0.001), breastfeeding <12 months (p=0.041) and shorter ideal IPI (p=0.03). Less than half of the women (43.3%, n=149) reported having received advice about IPI and less than half about postnatal contraception (44.2%, n=147).ConclusionsYounger age and non-use of LARC are significantly associated with IPIs <12 months. A minority of women with a short IPI perceived it to be ideal. Prevention of short IPIs could be achieved with improved access to postnatal contraception.
Survey on conduct of anaesthetic monitoring in small animal practice in the UK
Authors: Richardson Elisabeth Marie , McMillan Matthew 
Published: September 2019

BackgroundAn online survey was used to investigate current anaesthetic monitoring practices and the confidence level of personnel monitoring anaesthetics in small animal practices within the UK.MethodsVeterinary surgeons (VSs), registered veterinary nurses (RVNs) and student veterinary nurses (SVNs) working in the UK were invited to participate in an anonymous, internet‐based survey. To gather data, the questions used free text, multiple choice or scales measuring respondent attitude or opinion. No questions were mandatory and data were analysed with descriptive statistics or inductive thematic analysis.Results524 valid surveys were completed and included in the data analysis (VS n=136, RVN n=307, SVN n=81). The results indicated mainly RVNs perform pre‐anaesthetic monitoring equipment checks, set‐up the monitoring equipment and monitor anaesthesia and are more confident than VSs monitoring anaesthetics. VSs, RVNs and SVNs were all recognised to interpret and address changes in parameters monitored. Critical tasks pertaining to anaesthetic monitoring are being performed by personnel other than a VS, RVN or SVN. Respondents recognised the importance of monitoring in relation to patient outcome; however, a considerable proportion of respondents indicated that improving standards of monitoring was not a priority in their practice. Most respondents felt that standards of monitoring could be improved and that financial constraints were the major factor limiting improvement. Most respondents felt they would benefit from further training in anaesthetic monitoring.ConclusionVariability exists in how anaesthetic monitoring is conducted. Workplace pressures afflicting veterinary staff can influence the conduct of anaesthetic monitoring and initiating change within a veterinary practice can be difficult.
QED challenges at FCC-ee precision measurements
Authors: Jadach S. , Skrzypek M. 
Published: September 2019

Abstract
The expected experimental precision of the rates and asymmetries in the Future Circular Collider with electron–positron beams (FCC-ee) in the center of the mass energy range 88–365 GeV considered for construction in CERN, will be better by a factor 5–200. This will be thanks to the very high luminosity, a factor up to $$10^5$$105 higher than in the past LEP experiments. Consequently, it poses the extraordinary challenge of improving the precision of the Standard Model predictions by a comparable factor. In particular the perturbative calculations of the trivial QED effects, which have to be removed from the experimental data, are considered to be a major challenge for almost all quantities to be measured at FCC-ee. The task of this paper is to summarize the “state of the art” in this class of the calculations left over from the LEP era and to examine what is to be done to match the precision of the FCC-ee experiments – what kind of technical advancements are necessary. The above analysis will be done for most important observables of the FCC-ee, like the total cross sections near Z and WW threshold, charge asymmetries, the invisible width of Z boson, the spin asymmetry from $$\tau $$τ lepton decay and the luminosity measurement.
Anisotropic neutron stars and perfect fluid’s energy conditions
Authors: Setiawan A. M. , Sulaksono A. 
Published: September 2019

Abstract
It is reported (Estevez-Delgado and Estevez-Delgado in Eur Phys J C 78:673, 2018) recently, that the absence of anisotropic in compact object pressure leads to a solution which is not physically acceptable due to the energy density and speed of sound can not be positive at the origin at the same time. Here, we calculate the pressure and energy density of NSs using realistic EOS predicted by a relativistic mean-field model including hyperons for isotropic and three different anisotropic pressure models such as the one of Doneva and Yazadjiev (Phys Rev D 85:124023, 2012), the one of Herrera and Barreto (Phys Rev D 88:084022, 2013), and the one of Bowers and Liang (Astrophys J 188:657, 1974). We obtain that the all of energy conditions of perfect fluid are satisfied not only by anisotropic NSs profiles but also the one of isotropic NSs.
Aloe vera and its Components Inhibit Influenza A Virus-Induced Autophagy and Replication
Authors: Choi Jang-Gi , Lee Heeeun , Kim Young Soo , Hwang Youn-Hwan , Oh You-Chang , Lee Bonggi , Moon Kyoung Mi , Cho Won-Kyung , Ma Jin Yeul 
Published: September 2019

Aloe vera ethanol extract (AVE) reportedly has significant anti-influenza virus activity, but its underlying mechanisms of action and constituents have not yet been completely elucidated. Previously, we have confirmed that AVE treatment significantly reduces the viral replication of green fluorescent protein-labeled influenza A virus in Madin-Darby canine kidney (MDCK) cells. In addition, post-treatment with AVE inhibited viral matrix protein 1 (M1), matrix protein 2 (M2), and hemagglutinin (HA) mRNA synthesis and viral protein (M1, M2, and HA) expressions. In this study, we demonstrated that AVE inhibited autophagy induced by influenza A virus in MDCK cells and also identified quercetin, catechin hydrate, and kaempferol as the active antiviral components of AVE. We also found that post-treatment with quercetin, catechin hydrate, and kaempferol markedly inhibited M2 viral mRNA synthesis and M2 protein expression. A docking simulation suggested that the binding affinity of quercetin, catechin hydrate, and kaempferol for the M2 protein may be higher than that of known M2 protein inhibitors. Thus, the inhibition of autophagy induced by influenza virus may explain the antiviral activity of AVE against H1N1 or H3N2. Aloe vera extract and its constituents may, therefore, be potentially useful for the development of anti-influenza agents.
Human pancreatic afferent and efferent nerves: mapping and 3-D illustration of exocrine, endocrine, and adipose innervation
Authors: Chien Hung-Jen , Chiang Tsai-Chen , Peng Shih-Jung , Chung Mei-Hsin , Chou Ya-Hsien , Lee Chih-Yuan , Jeng Yung-Ming , Tien Yu-Wen , Tang Shiue-Cheng 
Published: September 2019

The pancreas consists of both the exocrine (acini and ducts) and endocrine (islets) compartments to participate in and regulate the body’s digestive and metabolic activities. These activities are subjected to neural modulation, but characterization of the human pancreatic afferent and efferent nerves remains difficult because of the lack of three-dimensional (3-D) image data. Here we prepare transparent human donor pancreases for 3-D histology to reveal the pancreatic microstructure, vasculature, and innervation in a global and integrated fashion. The pancreatic neural network consists of the substance P (SP)-positive sensory (afferent) nerves, the vesicular acetylcholine transporter (VAChT)-positive parasympathetic (efferent) nerves, and the tyrosine hydroxylase (TH)-positive sympathetic (efferent) nerves. The SP+ afferent nerves were found residing along the basal domain of the interlobular ducts. The VAChT+ and TH+ efferent nerves were identified at the peri-acinar and perivascular spaces, which follow the blood vessels to the islets. In the intrapancreatic ganglia, the SP+ (scattered minority, ~7%) and VAChT+ neurons co-localize, suggesting a local afferent-efferent interaction. Compared with the mouse pancreas, the human pancreas differs in 1) the lack of SP+ afferent nerves in the islet, 2) the lower ganglionic density, and 3) the obvious presence of VAChT+ and TH+ nerves around the intralobular adipocytes. The latter implicates the neural influence on the pancreatic steatosis. Overall, our 3-D image data reveal the human pancreatic afferent and efferent innervation patterns and provide the anatomical foundation for future high-definition analyses of neural remodeling in human pancreatic diseases. NEW & NOTEWORTHY Modern three-dimensional (3-D) histology with multiplex optical signals identifies the afferent and efferent innervation patterns of human pancreas, which otherwise cannot be defined with standard histology. Our 3-D image data reveal the unexpected association of sensory and parasympathetic nerves/neurons in the intrapancreatic ganglia and identify the sympathetic and parasympathetic nerve contacts with the infiltrated adipocytes. The multiplex approach offers a new way to characterize the human pancreas in remodeling (e.g., fatty infiltration and duct lesion progression).
Dietary bovine milk exosomes elicit changes in bacterial communities in C57BL/6 mice
Authors: Zhou Fang , Paz Henry A. , Sadri Mahrou , Cui Juan , Kachman Stephen D. , Fernando Samodha C. , Zempleni Janos 
Published: September 2019

Exosomes and exosome-like vesicles participate in cell-to-cell communication in animals, plant, and bacteria. Dietary exosomes in bovine milk are bioavailable in nonbovine species, but a fraction of milk exosomes reaches the large intestine. We hypothesized that milk exosomes alter the composition of the gut microbiome in mice. C57BL/6 mice were fed AIN-93G diets, defined by their content of bovine milk exosomes and RNA cargos: exosome/RNA-depleted (ERD) versus exosome/RNA-sufficient (ERS) diets. Feeding was initiated at age 3 wk, and cecum content was collected at ages 7, 15, and 47 wk. Microbial communities were identified by 16S rRNA gene sequencing. Milk exosomes altered bacterial communities in the murine cecum. The abundance of three phyla, seven families, and 52 operational taxonomic units (OTUs) was different in the ceca from mice fed ERD and ERS ( P < 0.05). For example, at the phylum level, Tenericutes had more than threefold abundance in ERS mice at ages 15 and 47 wk compared with ERD mice ( P < 0.05). At the family level, Verrucomicrobiaceae were much less abundant in ERS mice compared with ERD mice age 47 wk ( P < 0.05). At the OTU level, four OTUs from the family of Lachnospiraceae were more than two times more abundant in ERS mice compared with ERD at age 7 and 47 wk ( P < 0.05). We conclude that exosomes in bovine milk alter microbial communities in nonbovine species, suggesting that exosomes and their cargos participate in the crosstalk between bacterial and animal kingdoms. NEW & NOTEWORTHY This is the first report that exosomes from bovine milk alter microbial communities in mice. This report suggests that the gut microbiome facilitates cell-to-cell communication by milk exosomes across species boundaries, and milk exosomes facilitate communication across animal and bacteria kingdoms.
Rifaximin prevents ethanol-induced liver injury in obese KK-Ay mice through modulation of small intestinal microbiota signature
Authors: Kitagawa Ryuta , Kon Kazuyoshi , Uchiyama Akira , Arai Kumiko , Yamashina Shunhei , Kuwahara-Arai Kyoko , Kirikae Teruo , Ueno Takashi , Ikejima Kenichi 
Published: September 2019

Exacerbation of alcoholic hepatitis (AH) with comorbid metabolic syndrome is an emerging clinical problem, where microbiota plays a profound role in the pathogenesis. Here, we investigated the effect of rifaximin (RFX) on liver injury following chronic-binge ethanol (EtOH) administration in KK-Ay mice, a rodent model of metabolic syndrome. Female, 8-wk-old KK-Ay mice were fed Lieber–DeCarli diet (5% EtOH) for 10 days, following a single EtOH gavage (4 g/kg body wt). Some mice were given RFX (0.1 g/L, in liquid diet) orally. Small intestinal contents were collected from mice without binge. Intestinal microbiota was quantified using aerobic and anaerobic culturing techniques and further analyzed by 16S rRNA sequencing in detail. EtOH feeding/binge caused hepatic steatosis, oxidative stress, and induction of inflammatory cytokines in KK-Ay mice, which were markedly prevented by RFX treatment. Hepatic mRNA levels for cluster of differentiation 14, Toll-like receptor (TLR) 4, TLR2, and NADPH oxidase 2 were increased following EtOH feeding/binge, and administration of RFX completely suppressed their increase. The net amount of small intestinal bacteria was increased over threefold after chronic EtOH feeding as expected; however, RFX did not prevent this net increase. Intriguingly, the profile of small intestinal microbiota was dramatically changed following EtOH feeding in the order level, where the Erysipelotrichales predominated in the relative abundance. In sharp contrast, RFX drastically blunted the EtOH-induced increases in the Erysipelotrichales almost completely, with increased proportion of the Bacteroidales. In conclusion, RFX prevents AH through modulation of small intestinal microbiota/innate immune responses in obese KK-Ay mice. NEW & NOTEWORTHY Here we demonstrated that rifaximin (RFX) prevents chronic-binge ethanol (EtOH)-induced steatohepatitis in KK-Ay mice. Chronic EtOH feeding caused small intestinal bacterial overgrowth, with drastic alteration in the microbiota profile predominating the order Erysipelotrichales. RFX minimized this EtOH induction in Erysipelotrichales with substitutive increases in Bacteroidales. RFX also prevented EtOH-induced increases in portal lipopolysaccharide, and hepatic cluster of differentiation 14, toll-like receptor (TLR) 2, and TLR4 mRNA levels, suggesting the potential involvement of microbiota-related innate immune responses.
Recalibration of auditory perception of speech due to orofacial somatosensory inputs during speech motor adaptation
Authors: Ohashi Hiroki , Ito Takayuki 
Published: September 2019

Speech motor control and learning rely on both somatosensory and auditory inputs. Somatosensory inputs associated with speech production can also affect the process of auditory perception of speech, and the somatosensory-auditory interaction may play a fundamental role in auditory perception of speech. In this report, we show that the somatosensory system contributes to perceptual recalibration, separate from its role in motor function. Subjects participated in speech motor adaptation to altered auditory feedback. Auditory perception of speech was assessed in phonemic identification tests before and after speech adaptation. To investigate a role of the somatosensory system in motor adaptation and subsequent perceptual change, we applied orofacial skin stretch in either a backward or forward direction during the auditory feedback alteration as a somatosensory modulation. We found that the somatosensory modulation did not affect the amount of adaptation at the end of training, although it changed the rate of adaptation. However, the perception following speech adaptation was altered depending on the direction of the somatosensory modulation. Somatosensory inflow rather than motor outflow thus drives changes to auditory perception of speech following speech adaptation, suggesting that somatosensory inputs play an important role in tuning of perceptual system. NEW & NOTEWORTHY This article reports that the somatosensory system works not equally with the motor system, but predominantly in the calibration of auditory perception of speech by speech production.