bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding Parents’ Experiences and Information Needs on Pediatric Acute Otitis Media: A Qualitative Study
Authors: Meherali Salima , Campbell Alyson , Hartling Lisa , Scott Shannon 
Published: April 2018

Background: Acute otitis media (AOM) is the most common pediatric bacterial ear infection, affecting up to 75% of children younger than 5 years. Despite the high incidence of AOM in children, the condition presents a number of challenges to parents. The objectives of this study were to describe parents’ experiences of taking care of a child with AOM and to identify their information needs to manage their child with AOM. Methods: A qualitative, descriptive design was used to gain insight into information needs of parents’ of children with AOM. Participants were recruited from a specialized pediatric emergency department in a major Canadian urban center (Edmonton, Alberta). Individual semi structured interviews were conducted with 16 parents. Results: Seven major themes were identified and described: (1) frequency of AOM, (2) symptoms of AOM experienced by children and parents, (3) AOM symptom management strategies used by parents, (4) parent’s beliefs about AOM, (5) parent’s satisfaction with treatment prescribed by physicians, (6) the effect of AOM on family’s quality of life, and (7) parent’s information needs about AOM. Findings indicate that AOM has considerable negative outcomes for both children and families and that parents would benefit from having more evidence-based resources. Conclusions: This study provides important information around parents’ experiences and information needs for pediatric AOM. Identifying parents’ information needs and developing innovative and communicatively responsive educational approaches for parents are warranted that reflect patient-centered nursing care.
Red pulp macrophages in the human spleen are a distinct cell population with a unique expression of Fc-γ receptors
Authors: Nagelkerke Sietse Q. , Bruggeman Christine W. , den Haan Joke M. M. , Mul Erik P. J. , van den Berg Timo K. , van Bruggen Robin , Kuijpers Taco W. 
Published: April 2018

Key PointsHuman red pulp macrophages are distinct from monocytes and monocyte-derived macrophages in the expression of FcγRs and other surface markers. Red pulp macrophages phagocytose IgG-opsonized blood cells by activating FcγRs and are sensitive to IV immunoglobulin blocking
A systematic review of the epidemiology of carbapenem-resistant Enterobacteriaceae in the United States
Authors: Livorsi Daniel J. , Chorazy Margaret L. , Schweizer Marin L. , Balkenende Erin C. , Blevins Amy E. , Nair Rajeshwari , Samore Matthew H. , Nelson Richard E. , Khader Karim , Perencevich Eli N. 
Published: April 2018

Abstract
Background
Carbapenem-resistant Enterobacteriaceae (CRE) pose an urgent public health threat in the United States. An important step in planning and monitoring a national response to CRE is understanding its epidemiology and associated outcomes. We conducted a systematic literature review of studies that investigated incidence and outcomes of CRE infection in the US.

Methods
We performed searches in MEDLINE via Ovid, CDSR, DARE, CENTRAL, NHS EED, Scopus, and Web of Science for articles published from 1/1/2000 to 2/1/2016 about the incidence and outcomes of CRE at US sites.

Results
Five studies evaluated incidence, but many used differing definitions for cases. Across the entire US population, the reported incidence of CRE was 0.3–2.93 infections per 100,000 person-years. Infection rates were highest in long-term acute-care (LTAC) hospitals. There was insufficient data to assess trends in infection rates over time. Four studies evaluated outcomes. Mortality was higher in CRE patients in some but not all studies.

Conclusion
While the incidence of CRE infections in the United States remains low on a national level, the incidence is highest in LTACs. Studies assessing outcomes in CRE-infected patients are limited in number, small in size, and have reached conflicting results. Future research should measure a variety of clinical outcomes and adequately adjust for confounders to better assess the full burden of CRE.
Continuous increase of vancomycin resistance in enterococci causing nosocomial infections in Germany − 10 years of surveillance
Authors: Remschmidt Cornelius , Schröder Christin , Behnke Michael , Gastmeier Petra , Geffers Christine , Kramer Tobias Siegfried 
Published: April 2018

Abstract
Background
Enterococci are frequent pathogens causing nosocomial infections in Germany. Infections due to strains with vancomycin resistance are high when compared with other European states. Therefore, the study aimed to describe the recent progression of nosocomial infections due to vancomycin-resistant enterococci (VRE) in Germany.

Methods
We analyzed data from two components of the German national nosocomial infection surveillance system for the period 2007–2016. For primary bloodstream infections (BSIs) and urinary tract infections (UTIs) we used data from intensive care units and for surgical site infections (SSIs) data from surgical departments. In a sensitivity analysis, we considered only data from participants that participated continuously from 2007 to 2016 (“core group”). We calculated proportions of VRE among all nosocomial enterococcal infections with 95% confidence intervals (95% CIs) and trends over time. A multivariable logistic regression was used to compare occurrence of VRE proportions among German federal states.

Results
Enterococcal infections from 857 ICUs and 1119 surgical departments were analyzed. On ICUs, the proportion of vancomycin resistance in enterococci causing nosocomial infections significantly increased for BSIs from 5.9 to 16.7% and for UTIs from 2.9 to 9.9%; for surgical site infections, the proportion of VRE increased from 0.9 to 5.2% (P < 0.001 for all). In the core group, the increase of VRE was more pronounced in ICUs (BSIs: 5.5 to 21.6%; UTIs: 2 to 11.2%) but was not seen in surgical departments (SSIs: 1.5 to 2.8%). Compared with the most populous German federal state North Rhine Westphalia, enterococcal infections in Hesse (Odds Ratio (OR) 2.3, 95% CI 1.7–3.1), Saxony (OR 2.5, 95% CI 1.8–3.5) and Thuringia (OR 1.9, 95% CI 1.4–2.6) were more likely to be caused by vancomycin-resistant strains.

Conclusion
In Germany, the proportion of VRE in nosocomial infection due to enterococci is still increasing. It remains unclear, why a large variation in the proportion of VRE exists between German federal states.
Diabetes After Hormone Therapy in Breast Cancer Survivors: A Case-Cohort Study
Authors: Hamood Rola , Hamood Hatem , Merhasin Ilya , Keinan-Boker Lital 
Published: April 2018

Purpose Breast cancer treatments have been associated with an increased risk of multiple health-related adverse outcomes, but the relationship with diabetes remains unclear. This study investigated the association between hormone therapy and diabetes risk in breast cancer survivors. Patients and Methods We performed a case-cohort study of 2,246 female survivors recruited from the Leumit health care fund who were diagnosed with primary nonmetastatic invasive breast cancer in 2002 through 2012. A 20% random subcohort was sampled at baseline, and all diabetes cases were identified. Adjusted hazard ratios (HRs) with 95% CIs were estimated by weighted Cox proportional hazards regression models. Results Of 2,246 breast cancer survivors, 324 developed diabetes over a mean follow-up of 5.9 years. The crude cumulative incidence of diabetes that accounted for death as a competing risk was 20.9% (95% CI, 18.3% to 23.7%). In multivariable-adjusted models, hormone therapy was associated with increased diabetes risk (HR, 2.40; 95% CI, 1.26 to 4.55; P = .008). The hazard for tamoxifen use (HR, 2.25; 95% CI, 1.19 to 4.26; P = .013) was less pronounced than the use of aromatase inhibitors (HR, 4.27, 95% CI, 1.42 to 12.84; P = .010). Conclusion Active hormone therapy is a significant risk factor of diabetes among breast cancer survivors. Although cessation of treatment is not recommended because the survival benefits of hormone therapy outweigh the risks, preventive strategies aimed at lifestyle modifications may minimize the risk.
Multicenter Phase IB Trial of Carboxyamidotriazole Orotate and Temozolomide for Recurrent and Newly Diagnosed Glioblastoma and Other Anaplastic Gliomas
Authors: Omuro Antonio , Beal Kathryn , McNeill Katharine , Young Robert J. , Thomas Alissa , Lin Xuling , Terziev Robert , Kaley Thomas J. , DeAngelis Lisa M. , Daras Mariza , Gavrilovic Igor T. , Mellinghoff Ingo , Diamond Eli L. , McKeown Andrew , Manne Malbora , Caterfino Andrew , Patel Krishna , Bavisotto Linda , Gorman Greg , Lamson Michael , Gutin Philip , Tabar Viviane , Chakravarty Debyani , Chan Timothy A. , Brennan Cameron W. , Garrett-Mayer Elizabeth , Karmali Rashida A. , Pentsova Elena 
Published: April 2018

PurposeCarboxyamidotriazole orotate (CTO) is a novel oral inhibitor of non–voltage-dependent calcium channels with modulatory effects in multiple cell-signaling pathways and synergistic effects with temozolomide (TMZ) in glioblastoma (GBM) models. We conducted a phase IB study combining CTO with two standard TMZ schedules in GBM.MethodsIn cohort 1, patients with recurrent anaplastic gliomas or GBM received escalating doses of CTO (219 to 812.5 mg/m2once daily or 600 mg fixed once-daily dose) combined with TMZ (150 mg/m25 days during each 28-day cycle). In cohort 2, patients with newly diagnosed GBM received escalating doses of CTO (219 to 481 mg/m2/d once daily) with radiotherapy and TMZ 75 mg/m2/d, followed by TMZ 150 mg to 200 mg/m25 days during each 28-day cycle.ResultsForty-seven patients were enrolled. Treatment was well tolerated; toxicities included fatigue, constipation, nausea, and hypophosphatemia. Pharmacokinetics showed that CTO did not alter TMZ levels; therapeutic concentrations were achieved in tumor and brain. No dose-limiting toxicities were observed; the recommended phase II dose was 600 mg/d flat dose. Signals of activity in cohort 1 (n = 27) included partial (n = 6) and complete (n = 1) response, including in O6-methylguanine–DNA methyltransferase unmethylated and bevacizumab-refractory tumors. In cohort 2 (n = 15), median progression-free survival was 15 months and median overall survival was not reached (median follow-up, 28 months; 2-year overall survival, 62%). Gene sequencing disclosed a high rate of responses among EGFR-amplified tumors ( P = .005), with mechanisms of acquired resistance possibly involving mutations in mismatch-repair genes and/or downstream components TSC2, NF1, NF2, PTEN, and PIK3CA.ConclusionCTO can be combined safely with TMZ or chemoradiation in GBM and anaplastic gliomas, displaying favorable brain penetration and promising signals of activity in this difficult-to-treat population.
Building a plant cell wall at a glance
Authors: Lampugnani Edwin R. , Khan Ghazanfar Abbas , Somssich Marc , Persson Staffan 
Published: April 2018

ABSTRACT
Plant cells are surrounded by a strong polysaccharide-rich cell wall that aids in determining the overall form, growth and development of the plant body. Indeed, the unique shapes of the 40-odd cell types in plants are determined by their walls, as removal of the cell wall results in spherical protoplasts that are amorphic. Hence, assembly and remodeling of the wall is essential in plant development. Most plant cell walls are composed of a framework of cellulose microfibrils that are cross-linked to each other by heteropolysaccharides. The cell walls are highly dynamic and adapt to the changing requirements of the plant during growth. However, despite the importance of plant cell walls for plant growth and for applications that we use in our daily life such as food, feed and fuel, comparatively little is known about how they are synthesized and modified. In this Cell Science at a Glance article and accompanying poster, we aim to illustrate the underpinning cell biology of the synthesis of wall carbohydrates, and their incorporation into the wall, in the model plant Arabidopsis.
CRL7SMU1 E3 ligase complex-driven H2B ubiquitylation functions in sister chromatid cohesion by regulating SMC1 expression
Authors: Shah Varun Jayeshkumar , Maddika Subbareddy 
Published: April 2018

ABSTRACT
Cullin–RING-type E3 ligases (CRLs) control a broad range of biological processes by ubiquitylating numerous cellular substrates. However, the role of CRL E3 ligases in chromatid cohesion is unknown. In this study, we identified a new CRL-type E3 ligase (designated as CRL7SMU1 complex) that has an essential role in the maintenance of chromatid cohesion. We demonstrate that SMU1, DDB1, CUL7 and RNF40 are integral components of this complex. SMU1, by acting as a substrate recognition module, binds to H2B and mediates monoubiquitylation at the lysine (K) residue K120 through CRL7SMU1 E3 ligase complex. Depletion of CRL7SMU1 leads to loss of H2B ubiquitylation at the SMC1a locus and, thus, subsequently compromised SMC1a expression in cells. Knockdown of CRL7SMU1 components or loss of H2B ubiquitylation leads to defective sister chromatid cohesion, which is rescued by restoration of SMC1a expression. Together, our results unveil an important role of CRL7SMU1 E3 ligase in promoting H2B ubiquitylation for maintenance of sister chromatid cohesion during mitosis.
This article has an associated First Person interview with the first author of the paper.
The Problem with “Magnitude-based Inference”
Authors: SAINANI KRISTIN L. 
Published: April 2018

ABSTRACT

Purpose
A statistical method called “magnitude-based inference” (MBI) has gained a following in the sports science literature, despite concerns voiced by statisticians. Its proponents have claimed that MBI exhibits superior type I and type II error rates compared with standard null hypothesis testing for most cases. I have performed a reanalysis to evaluate this claim.

Methods
Using simulation code provided by MBI’s proponents, I estimated type I and type II error rates for clinical and nonclinical MBI for a range of effect sizes, sample sizes, and smallest important effects. I plotted these results in a way that makes transparent the empirical behavior of MBI. I also reran the simulations after correcting mistakes in the definitions of type I and type II error provided by MBI’s proponents. Finally, I confirmed the findings mathematically; and I provide general equations for calculating MBI’s error rates without the need for simulation.

Results
Contrary to what MBI’s proponents have claimed, MBI does not exhibit “superior” type I and type II error rates to standard null hypothesis testing. As expected, there is a tradeoff between type I and type II error. At precisely the small-to-moderate sample sizes that MBI’s proponents deem “optimal,” MBI reduces the type II error rate at the cost of greatly inflating the type I error rate—to two to six times that of standard hypothesis testing.

Conclusions
Magnitude-based inference exhibits worrisome empirical behavior. In contrast to standard null hypothesis testing, which has predictable type I error rates, the type I error rates for MBI vary widely depending on the sample size and choice of smallest important effect, and are often unacceptably high. Magnitude-based inference should not be used.
Evaluation of an internet-based animated preparatory video for children undergoing non-sedated MRI
Authors: McGlashan Hannah L , Dineen Rob A , Szeszak Sofia , Whitehouse William P , Chow Gabriel , Love Andrew , Langmack Gill , Wharrad Heather 
Published: April 2018

Objectives:
We evaluate the value of an internet-based educational animated video designed to prepare children for MRI scans, and whether this video reduces scan-related anxiety in children with a neurological disorder, and healthy controls.

Methods:
Participants completed a pre- and post-scan questionnaire evaluating participant online viewing behaviour, understanding of the MRI procedure, anxiety regarding the MRI, impact of animation in preparing the child and whether the child’s expectation of the MRI scan matched their experience.

Results:
21 children were recruited (12 healthy controls) ranging in age from 6.5 to 11.5 years. The animation was successfully accessed by participants on a range of digital devices and had high levels of approval. Children who viewed the animation had a good understanding of the MRI procedure and low anxiety levels prior to the scan, and reported that their expectations broadly matched the real-life MRI experience. Children reported that the animation positively impacted on their preparation with similar ratings before and after the scan, and the impact on preparation was rated greater by younger children. There were no group differences between healthy children and those with the neurological disorder for ratings of anxiety, impact on preparation and expectation of the experience.

Conclusion:
This evaluation demonstrates accessibility, acceptability and relevance of internet-based educational animation for typically developing children, and children with a neurodisability aged 6 to 11 years, with positive impact on preparation for MRI.

Advances in knowledge:
The internet-based educational animation provides a widely accessible tool to support preparation of children for non-sedated MRI.
Emerging therapies for hemophilia: controversies and unanswered questions
Authors: Arruda Valder R. , Doshi Bhavya S. , Samelson-Jones Benjamin J. 
Published: April 2018

Several new therapies for hemophilia have emerged in recent years. These strategies range from extended half-life factor replacement products and non-factor options with improved pharmacokinetic profiles to gene therapy aiming for phenotypic cure. While these products have the potential to change hemophilia care dramatically, several challenges and questions remain regarding broader applicability, long-term safety, and which option to pursue for each patient. Here, we review these emerging therapies with a focus on controversies and unanswered questions in each category.
Stage 1 Registered Report: Variation in neurodevelopmental outcomes in children with sex chromosome trisomies: protocol for a test of the double hit hypothesis
Authors: Newbury Dianne F. , Simpson Nuala H. , Thompson Paul A. , Bishop Dorothy V. M. 
Published: April 2018

Background: The presence of an extra sex chromosome is associated with an increased rate of neurodevelopmental difficulties involving language. Group averages, however, obscure a wide range of outcomes. Hypothesis: The 'double hit' hypothesis proposes that the adverse impact of the extra sex chromosome is amplified when genes that are expressed from the sex chromosomes interact with autosomal variants that usually have only mild effects. Neuroligin-4 genes are expressed from X and Y chromosomes; they play an important role in synaptic development and have been implicated in neurodevelopment. We predict that the impact of an additional sex chromosome on neurodevelopment will be correlated with common autosomal variants involved in related synaptic functions. We describe here an analysis plan for testing this hypothesis using existing data. The analysis of genotype-phenotype associations will be conducted after this plan is published and peer-reviewed Methods: Neurodevelopmental data and DNA are available for 130 children with sex chromosome trisomies (SCTs: 42 girls with trisomy X, 43 boys with Klinefelter syndrome, and 45 boys with XYY). Children from a twin study using the same phenotype measures will form two comparison groups (Ns = 184 and 186). Three indicators of a neurodevelopment disorder phenotype will be used: (i) Standard score on a test of nonword repetition; (ii). A language factor score derived from a test battery; (iii) A general scale of neurodevelopmental challenges based on all available information. Autosomal genes were identified by literature search on the basis of prior association with (a) speech/language/reading phenotypes and (b) synaptic function. Preselected regions of two genes scoring high on both criteria, CNTNAP2 and NRXN1, will be tested for association with neurodevelopmental outcomes using Generalised Structural Component Analysis. We predict the association with one or both genes will be detectable in children with SCTs and stronger than in the comparison samples.
Analysis of Microclimate and Cucumber Fruit Yield in a Screenhouse and an Evaporatively Cooled Greenhouse in a Semi-Arid Location
Authors: Al-Mulla Yaseen A. , Al-Balushi Mohammed I. , Al-Busaidi Hamad A. , Al-Mahdouri Adil A. , Kittas Constantinos , Katsoulas Nikolaos 
Published: April 2018

Abstract. The microclimate and cucumber crop response in a screenhouse and in an evaporatively cooled greenhouse were studied in Oman during winter/spring and spring/summer cultivation periods. Measurements were carried out in two similarly shaped structures: (1) a greenhouse equipped with a pad-and-fan system for evaporative cooling of the greenhouse environment and (2) a screenhouse with no cooling system. Analysis of the spring/summer period climate data showed that the evaporative cooling in the greenhouse reduced the mean air temperature by about 4.5°C compared to outside and maintained the leaf temperature close to the greenhouse air temperature. The 24 h mean leaf and air temperatures in the greenhouse reached 25.8°C ±1.3°C and 25.9°C ±0.8°C, respectively. On the other hand, the 24 h mean leaf and air temperatures in the screenhouse were higher by 1.0°C and 1.3°C, respectively, compared to outside. The 24 h mean leaf and air temperatures in the screenhouse reached 32.8°C ±1.2°C and 31.8°C ±1.5°C, respectively. Furthermore, the evaporative cooling in the greenhouse maintained the 24 h mean air vapor pressure deficit (VPD) values at levels lower than 1.1 kPa, while the 24 h mean air VPD in the screenhouse reached values up to 4.5 kPa. These differences resulted in a 50% decrease in crop fruit yield during the spring/summer period. The radiation and water use efficiency (WUE) values observed in the two structures were similar during the winter/spring period but were higher in the greenhouse during the spring/summer period. However, for the greenhouse, when the water evaporated in the wet pad was also considered, the overall WUE was at the same level in both structures during summer. Furthermore, the evaporative cooling applied in the greenhouse enhanced the mean values of fruit quality characteristics measured during the spring/summer, such as fruit dry matter content (5.6%), fruit firmness (5.0 kg cm-2), and chroma (18.6), compared to that of the screenhouse (5.0%, 4.9 kg cm-2 and 16.3, respectively), but did not significantly affect other fruit quality characteristics, such as mean fruit weight (128 g for greenhouse and 123 g for screenhouse), total soluble solids content (3.9 °Brix for both structures), and juice pH (5.7 for greenhouse and 5.6 for screenhouse). Overall, it can be concluded that under the weather conditions of Oman, although greenhouses are still needed during spring/summer, screenhouses can be used during winter without jeopardizing crop production quantity and quality. Keywords: Evaporative cooling, Evapotranspiration, Radiation use efficiency, Water user efficiency. Total water use efficiency, Climate.