bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rational Identification of a Colorectal Cancer Targeting Peptide through Phage Display
Authors: Ferreira Débora , Silva Ana P. , Nobrega Franklin L. , Martins Ivone M. , Barbosa-Matos Catarina , Granja Sara , Martins Sandra F. , Baltazar Fátima , Rodrigues Ligia R. 
Published: March 2019

AbstractColorectal cancer is frequently diagnosed at an advanced stage due to the absence of early clinical indicators. Hence, the identification of new targeting molecules is crucial for an early detection and development of targeted therapies. This study aimed to identify and characterize novel peptides specific for the colorectal cancer cell line RKO using a phage-displayed peptide library. After four rounds of selection plus a negative step with normal colorectal cells, CCD-841-CoN, there was an obvious phage enrichment that specifically bound to RKO cells. Cell-based enzyme-linked immunosorbent assay (ELISA) was performed to assess the most specific peptides leading to the selection of the peptide sequence CPKSNNGVC. Through fluorescence microscopy and cytometry, the synthetic peptide RKOpep was shown to specifically bind to RKO cells, as well as to other human colorectal cancer cells including Caco-2, HCT 116 and HCT-15, but not to the normal non-cancer cells. Moreover, it was shown that RKOpep specifically targeted human colorectal cancer cell tissues. A bioinformatics analysis suggested that the RKOpep targets the monocarboxylate transporter 1, which has been implicated in colorectal cancer progression and prognosis, proven through gene knockdown approaches and shown by immunocytochemistry co-localization studies. The peptide herein identified can be a potential candidate for targeted therapies for colorectal cancer.
Decreased alpha-band oscillatory brain activity prior to movement initiated by perception of fatigue sensation
Authors: Ishii Akira , Matsuo Takashi , Nakamura Chika , Uji Masato , Yoshikawa Takahiro , Watanabe Yasuyoshi 
Published: March 2019

AbstractFatigue is a health problem prevalent in modern societies. Fatigue sensation plays an important role as a biological alarm urging rest to maintain homeostasis, and clarifying the neural mechanisms related to fatigue sensations by which we decide to engage in rest is therefore essential. This study enrolled healthy male volunteers and showed that the decrease in alpha-band power as assessed by magnetoencephalography of the left Brodmann’s area (BA) 6 before perception of fatigue when a button-press based on the level of fatigue was required was smaller than that before perception of the intention to move when a voluntary button-press was required. In addition, the decrease of alpha-band power in the left BA 6 before the perception of fatigue was not altered compared with that in the right BA 6 when a button-press based on the level of fatigue was required. These results suggest that the button-press based on the perception of fatigue is not prepared before the perception of fatigue. These findings will advance the understanding of the neural mechanisms related to subjective feelings such as fatigue sensation.
Infection pattern and negative effects of a facultative endosymbiont on its insect host are environment-dependent
Authors: Liu Xiang-Dong , Lei Hai-Xia , Chen Fang-Fang 
Published: March 2019

AbstractRegiella insecticolais a bacterial endosymbiont in insects that exhibits a negative effect on the fitness of hosts. Thus, it is not clear why this costly endosymbiont can persist in host populations. Here, we tested a hypothesis that the infection pattern and negative roles of the endosymbiont were not constant but environmentally dependent. The grain aphidsSitobion avenae, belonging to different genotypes and infected withRegiellaor not, were used in this study. We found thatS.avenaepopulations were infected withRegiella,Hamiltonella defensa,Serratia symbioticaandRickettsia. The predominant endosymbionts in the aphid populations varied with season.SerratiaandRickettsiawere predominant from December to February whileRegiellapredominated from March to May. The vertical transmission ofRegiellawas poorer at high temperature, but following conditioning for seven generations, the transmission rate improved.Regiellainhibited the production of winged aphids at 25 °C, but it did not affect winged morph production at the higher temperatures of 28 °C and 31 °C.Regiellainfection decreased the intrinsic rate of increase (rm) of aphids at 25 °C and 28 °C. However, at 31 °C, the effect ofRegiellaon thermvaried depending on the aphid genotype and density. Thus, the negative effects of this endosymbiont on its host were environmentally dependent.
A Novel Lactate Dehydrogenase Inhibitor, 1-(Phenylseleno)-4-(Trifluoromethyl) Benzene, Suppresses Tumor Growth through Apoptotic Cell Death
Authors: Kim Eun-Yeong , Chung Tae-Wook , Han Chang Woo , Park So Young , Park Kang Hyun , Jang Se Bok , Ha Ki-Tae 
Published: March 2019

AbstractThe Warburg effect, wherein cancer cells prefer glycolysis rather than oxidative phosphorylation even under normoxic conditions, is a major characteristic of malignant tumors. Lactate dehydrogenase A (LDHA) is the main enzyme regulating the Warburg effect, and is thus, a major target for novel anti-cancer drug development. Through our ongoing screening of novel inhibitors, we found that several selenobenzene compounds have inhibitory effects on LDHA activity. Among them, 1-(phenylseleno)-4-(trifluoromethyl) benzene (PSTMB) had the most potent inhibitory effect on the enzymatic activity of LDHA. The results from biochemical assays and computational modeling showed that PSTMB inhibited LDHA activity. In addition, PSTMB inhibited the growth of several tumor cell lines, including NCI-H460, MCF-7, Hep3B, A375, HT29, and LLC. In HT29 human colon cancer cells, PSTMB dose-dependently inhibited the viability of the cells and activity of LDHA, without affecting the expression of LDHA. Under both normoxic and hypoxic conditions, PSTMB effectively reduced LDHA activity and lactate production. Furthermore, PSTMB induced mitochondria-mediated apoptosis of HT29 cells via production of reactive oxygen species. These results suggest that PSTMB may be a novel candidate for development of anti-cancer drugs by targeting cancer metabolism.
Systematic Review and Meta-analysis on the Effect of Soy on Thyroid Function
Authors: Otun Jemiliat , Sahebkar Amirhossein , Östlundh Linda , Atkin Stephen L. , Sathyapalan Thozhukat 
Published: March 2019

AbstractSoy foods have had an important dietary role in Asian countries for centuries, and in recent years they have become increasingly popular in Western countries as a result of their suggested health benefits. Nevertheless, there are some concerns that soy can have a negative effect on thyroid function and can alter the levels of thyroid hormones. The aim of this systematic review was to investigate the link between soy or soy product consumption and thyroid function via the measurement of thyroid hormone levels. A systematic review and meta-analysis was undertaken on all randomised controlled trials of studies including soy as an intervention and where free triiodothyronine (fT3), free thyroxine (fT4) and thyroid stimulating hormone (TSH) was measured. The search included PubMed, MEDLINE, EMBASE, Cochrane and sources for the grey literature. Quantitative data synthesis was performed using a random-effects model, with standardized mean difference (SMD) and 95% confidence interval as summary statistics. A total of 18 articles were suitable for review. The meta-analysis showed no significant changes in fT3 (WMD: 0.027 pmol/L, 95% CI: −0.052, 0.107, p = 0.499; I2: 55.58%), fT4 (WMD: −0.003 pmol/L, 95% CI: −0.018, 0.011, p = 0.656; I2: 87.58%) while an elevation in TSH levels was observed (WMD: 0.248 mIU/L, 95% CI: 0.001, 0.494, p = 0.049; I2: 80.31%) levels with soy supplementation. There was no evidence of publication bias. Soy supplementation has no effect on the thyroid hormones and only very modestly raises TSH levels, the clinical significance, if any, of the rise in TSH is unclear.
Vitrification of canine ovarian tissues with polyvinylpyrrolidone preserves the survival and developmental capacity of primordial follicles
Authors: Fujihara Mayako , Kaneko Takehito , Inoue-Murayama Miho 
Published: March 2019

AbstractOvarian tissue cryopreservation combined with immature follicle development can preserve female fertility in wildlife, regardless of age or reproductive timing. To investigate the effects of different cryopreservation methods and cryoprotectants on follicular survival and developmental capacity, ovarian cortical pieces from 15 dogs were cryopreserved by slow freezing or vitrification with different additional cryoprotectants as follows: dimethyl sulfoxide (DMSO), polyvinylpyrrolidone (PVP), combined DMSO and PVP (each at half the concentration of when used independently), or none (control). Cryopreserved ovarian tissues were evaluated by neutral red staining, histology, and xenotransplantation assays. Among cryopreservation conditions tested, vitrification with combined DMSO and PVP significantly improved the maintenance of follicular morphology compared to that in control. Furthermore, ovarian tissues vitrified using this condition maintained follicle morphology and developmental capacity 9 weeks after grafting, as shown by an increased percentage of primary and secondary follicles and a significant decrease in the transition stage from primordial to primary stage follicles 5 and 9 weeks after grafting. In contrast, slow freezing and control groups lost intact follicles by 5 weeks after grafting. The described cryopreservation techniques, which preserve canine follicle development, will build the foundation of ovarian tissue cryopreservation to preserve female fertility in wild canids.
Cholecystokinin induces crowing in chickens
Authors: Shimmura Tsuyoshi , Tamura Mai , Ohashi Shosei , Sasaki Asuka , Yamanaka Takamichi , Nakao Nobuhiro , Ihara Kunio , Okamura Shinsaku , Yoshimura Takashi 
Published: March 2019

AbstractAnimals that communicate using sound are found throughout the animal kingdom. Interestingly, in contrast to human vocal learning, most animals can produce species-specific patterns of vocalization without learning them from their parents. This phenomenon is called innate vocalization. The underlying molecular basis of both vocal learning in humans and innate vocalization in animals remains unknown. The crowing of a rooster is also innately controlled, and the upstream center is thought to be localized in the nucleus intercollicularis (ICo) of the midbrain. Here, we show that the cholecystokinin B receptor (CCKBR) is a regulatory gene involved in inducing crowing in roosters. Crowing is known to be a testosterone (T)-dependent behavior, and it follows that roosters crow but not hens. Similarly, T-administration induces chicks to crow. By using RNA-sequencing to compare gene expression in the ICo between the two comparison groups that either crow or do not crow, we found that CCKBR expression was upregulated in T-containing groups. The expression of CCKBR and its ligand, cholecystokinin (CCK), a neurotransmitter, was observed in the ICo. We also showed that crowing was induced by intracerebroventricular administration of an agonist specific for CCKBR. Our findings therefore suggest that the CCK system induces innate vocalization in roosters.
Multi-metal 4D printing with a desktop electrochemical 3D printer
Authors: Chen Xiaolong , Liu Xinhua , Ouyang Mengzheng , Chen Jingyi , Taiwo Oluwadamilola , Xia Yuhua , Childs Peter R. N. , Brandon Nigel P. , Wu Billy 
Published: March 2019

Abstract4D printing has the potential to create complex 3D geometries which are able to react to environmental stimuli opening new design possibilities. However, the vast majority of 4D printing approaches use polymer based materials, which limits the operational temperature. Here, we present a novel multi-metal electrochemical 3D printer which is able to fabricate bimetallic geometries and through the selective deposition of different metals, temperature responsive behaviour can thus be programmed into the printed structure. The concept is demonstrated through a meniscus confined electrochemical 3D printing approach with a multi-print head design with nickel and copper used as exemplar systems but this is transferable to other deposition solutions. Improvements in deposition speed (34% (Cu)–85% (Ni)) are demonstrated with an electrospun nanofibre nib compared to a sponge based approach as the medium for providing hydrostatic back pressure to balance surface tension in order to form a electrolyte meniscus stable. Scanning electron microscopy, X-ray computed tomography and energy dispersive X-ray spectroscopy shows that bimetallic structures with a tightly bound interface can be created, however convex cross sections are created due to uneven current density. Analysis of the thermo-mechanical properties of the printed strips shows that mechanical deformations can be generated in Cu-Ni strips at temperatures up to 300 °C which is due to the thermal expansion coefficient mismatch generating internal stresses in the printed structures. Electrical conductivity measurements show that the bimetallic structures have a conductivity between those of nanocrystalline copper (5.41 × 106 S.m−1) and nickel (8.2 × 105 S.m−1). The potential of this novel low-cost multi-metal 3D printing approach is demonstrated with the thermal actuation of an electrical circuit and a range of self-assembling structures.
The Escherichia coli multiple antibiotic resistance activator protein represses transcription of the lac operon
Authors: Lankester Anna , Ahmed Shafayeth , Lamberte Lisa E. , Kettles Rachel A. , Grainger David C. 
Published: March 2019

Abstract
In Escherichia coli, the marRAB operon is a determinant for antibiotic resistance. Such phenotypes require the encoded transcription factor MarA that activates efflux pump expression. To better understand all genes controlled by MarA, we recently mapped binding of the regulator across the E. coli genome. As expected, many MarA targets were adjacent to genes encoding stress response systems. Surprisingly, one MarA-binding site overlapped the lac operon regulatory region. Here, we show that MarA specifically targets this locus and can block transcription of the lac genes. Repression is mediated by binding of MarA to a site overlapping the lacP1 promoter −35 element. Control of the lac operon by MarA does not impact antibiotic resistance.
Innenohrschwerhörigkeit und
Gleichgewichtsstörungen
Authors: Merkt W. , Lorenz H. M. , Blank N. 
Published: March 2019

EinleitungDas Cogan-Syndrom ist eine seltene Erkrankung, die durch eine bilaterale
Innenohrschwerhörigkeit, eine Störung des Gleichgewichtsorganes mit Ataxie und
eine interstitielle Keratitis charakterisiert ist. In einigen Fällen wurde eine
Assoziation mit einer systemischen Vaskulitis beschrieben. Eine wirksame
Therapie zur Erhaltung des Hörvermögens ist bisher nicht bekannt. Der Verlust
des Hörvermögens wird häufig durch eine Versorgung mit Hörgeräten kompensiert.
In diesem Fallbericht beschreiben wir eine deutliche Besserung des Hörvermögens
durch eine Therapie mit dem Interleukin (IL)-1-Rezeptorantagonisten
Anakinra.
Winkelstabile Plattenosteosynthese
Authors: Rußow Gabriele , Schütz Michael , Berner Arne 
Published: March 2019

ZusammenfassungDie winkelstabile Plattenosteosynthese ist aus der modernen Unfallchirurgie und
Orthopädie nicht mehr wegzudenken. Sie hat unterschiedliche Entwicklungsschritte
durchgemacht, bis sie schließlich bei dem Produkt angekommen ist, das heute so
weit verbreitet ist. Dieser Artikel liefert eine Einführung in die Grundlagen
der winkelstabilen Plattenosteosynthese.
Was essen nach Tonsillektomie?
Authors: Gerstner Andreas O. H. 
Published: March 2019

Faramarzi M et al. Comparing Cold/Liquid Diet vs Regular Diet on
Posttonsillectomy Pain and Bleeding. Otolaryngol Head Neck Surg 2018; 159:
755–760
Die Tonsillektomie ist eine häufige Operation. Hinsichtlich
Nahrungsmittelauswahl und Art der Ernährung nach der Tonsillektomie gehen
die Meinungen aber auseinander. Ärzte des HNO-Forschungszentrums in Schiras
im Iran verglichen die Auswirkungen einer kalt/flüssigen Diät und einer
normalen Ernährung auf Schmerzen und Blutungen im Bereich der
Tonsillektomie.
Wie viele Pflegende braucht eine Klinik?
Authors: Eck Ingo 
Published: March 2019

ZusammenfassungEine bessere Personalausstattung bringt Entlastung für alle Pflegenden – doch wie
lässt sich der tatsächlich erforderliche Personalbedarf einer Klinik ermitteln?
Speziell für Intensivstationen hat ein Pflegeteam unter der Leitung von Ingo Eck
ein EDV-basiertes System zur genauen Leistungserfassung entwickelt.
INPULS® kommt mittlerweile in 26 Kliniken deutschlandweit zum
Einsatz und wird ständig weiterentwickelt. Wir stellen das System vor.
Perioperative Care of Free Flap Patients
Authors: Vincent Aurora , Sawhney Raja , Ducic Yadranko 
Published: March 2019

AbstractMicrovascular free tissue transfer is an indispensable reconstructive option in head and neck reconstruction. Flap failure is relatively rare, but it is nonetheless very morbid and psychologically devastating to patients when it does occur. Further, complications after free tissue transfer to the head and neck remain common. There are numerous ongoing debates about various facets of preoperative, intraoperative, and postoperative care of patients undergoing free flap reconstruction of the head and neck, all ultimately searching for the optimal treatment algorithm to further improve flap success, minimize complications, and maximize patient outcomes. Herein, the authors review current literature surrounding optimal preoperative nutritional support, intraoperative vasopressor use, perioperative fluid management, use of antithrombotic agents, antibiotic use, and other facets of the care of head and neck free flap patients to provide a guide to surgeons.
RNA-Sequencing of Umbilical Cord Blood to Investigate Spontaneous Preterm Birth: A Pilot Study
Authors: Vora Neeta , Parker Joel , Mieckowski Piotr , Smeester Lisa , Fry Rebecca , Boggess Kim 
Published: March 2019

Objective To analyze the transcriptomic gene expression of umbilical cord blood leukocytes using RNA-sequencing from preterm birth (PTB) and term birth (TB).
Study Design Eight women with spontaneous PTB (sPTB) and eight women with unlabored TB were enrolled prospectively. The sPTB and TB cohorts were matched for maternal age, race, mode of delivery, and fetal sex. Cord blood RNA was extracted and a globin depletion protocol was applied, then sequenced on the Illumina HiSeq 4000. Raw read counts were analyzed with DESeq2 to test for gene expression differences between sPTB and TB.
Results 148 genes had significant differential expression (q < 0.01). Cell cycle/metabolism gene expression was significantly higher and immune/inflammatory signaling gene expression significantly lower in the sPTB cohort compared with term. In African American (AA) infants, 18 genes specific to cell signaling, neutrophil activity, and major histocompatibility complex type 1 had lower expression in preterm compared with term cohort; the opposite pattern was seen in non-Hispanic Whites (NHWs).
Conclusion Compared with term, preterm fetuses have higher cell cycle/metabolism gene expression, suggesting metabolic focus on growth and development. Immune function gene expression in this pilot study is lower in the sPTB group compared with term and differs in AA compared with NHW infants.
A Pushed Descending Aorta due to Hiatal Hernia
Authors: Gecmen Cetin , Kahyaoglu Muzaffer , Kalayci Arzu , Cakmak Ender , Candan Ozkan , Guner Ahmet , Izgi Ibrahim , Kirma Cevat 
Published: March 2019

AbstractA 91-year-old female patient was admitted to hospital for evaluation of transcatheter aortic valve implantation. A chest radiography showed a hiatal hernia, and the stomach and duodenum appeared in the thoracic cavity. The descending aorta was pushed by the stomach and duodenum.
Chondroblastoma of the Femoral Head: A Case Series and Literature Review
Authors: Hoskins Tyler , Sonnylal Laura , O'Connor Ian , Patel Jay , Issa Kimona , Wittig James 
Published: March 2019

AbstractChondroblastoma is a rare bone tumor predominantly affecting patients during the first and second decades of life. The purpose of this case series is to assist clinicians in establishing a proper diagnosis and treatment method for patients presenting with chondroblastoma of the femoral head. Symptoms of this tumor in the hip joint can be nonspecific, resulting in the critical delay of a proper diagnosis. Due to the aggressive nature of this tumor, an early diagnosis is vital to prevent significant local bone destruction and the potential loss of a mobile hip. The authors present a series of three adolescent patients treated by the primary investigator between 2014 and 2017 for chondroblastoma of the femoral head to help illustrate several diagnostic and treatment principles and considerations for clinicians. The mean Musculoskeletal Tumor Society score at the latest follow-up was 26 points (range: 25–27). No local recurrence was reported in any patient and all individuals have returned to preoperative function, and are participating in daily activities and sports.
Health-related Quality of Life in Women with Cervical Cancer
Authors: dos Santos Larissa Nascimento , Castaneda Luciana , de Aguiar Suzana Sales , Thuler Luiz Claudio Santos , Koifman Rosalina Jorge , Bergmann Anke 
Published: March 2019

Objective To analyze the factors associated with health-related quality of life (HRQoL) in women with cervical cancer (CC) in a single center in Rio de Janeiro, state of Rio de Janeiro, Brazil.
Methods A cross-sectional study in women with a diagnosis of CC followed-up in the gynecology outpatient clinic of the Hospital do Câncer II (HCII, in the Portuguese acronym) of the Instituto Nacional de Câncer (INCA, in the Portuguese acronym). The data were collected from March to August 2015. Women with palliative care, communication/cognition difficulty, undergoing simultaneous treatment for other types of cancer, or undergoing chemotherapy and/or radiation therapy were excluded. For the evaluation of the HRQoL, a specific questionnaire for women with CC was used (Functional Assessment of Cancer Therapy – Cervix Cancer [FACT-Cx]). The total score of the questionnaire ranges from 0 to 168, with higher scores indicating a better HRQoL.
Results A total of 115 women were included in the present study, with a mean age of 52.64 years old (standard deviation [SD] = 12.13). The domains of emotional (16.61; SD = 4.55) and functional well-being (17.63; SD = 6.15) were those which presented the worst scores. The factors that had an association with better HRQoL in women with CC were having a current occupation, a longer time since the treatment and diagnosis, and women who had undergone hysterectomy.
Conclusion Considering the domains of HRQoL of the women treated for cervical cancer, a better score was observed in the domains of physical and social/family well-being. For most domains, better scores were found between those with a current occupation, with a longer time after the diagnosis and treatment, and among those who had undergone a hysterectomy.