bluuebunny/crossref_metadata_2025_split huggingface dataset:
Numerical Version of the Complex-Scaling Green’s Operator Method
Authors: Yamasaki N , Fumimoto T , Maeda T , Ito M 
Published: November 2024

Abstract
A new treatment of scattering problems is formulated by improving the complex-scaling Green’s operator (CSGO) method, which can calculate the transition or scattering matrix from the spectral representation of the complex-scaled Green’s function and the asymptotic solution expressed in terms of the analytic function. In the present formulation, the CSGO method is extended to handle the general scattering problem in charged-particle systems by employing the Coulomb wave function numerically calculated under the complex-scaled asymptotic Hamiltonian. We call the new method the CSGO-numerical (CSGON) method, and it is applied to the scattering problem of the $^4$He + proton system to check its validity. The continuum level density (CLD) method, which is another method to solve the scattering problem, is also applied to the same system. The numerical precision and the applicability of the CSGON method is discussed by comparing it with the CLD and CSGO methods.
eIF6 modulates skin wound healing by upregulating keratin 6B
Authors: Wang Xiaoyan , Xu Guangchao , Zhang Fangyingnan , Wei Yating , Deng Jiawen , Mu Lan , He Jinqing , He Dehua , Yin Meifang , Dal Pra Ilaria , Liu Xiaofang , Cai Weichao , Yang Linjing , Han Chunmao , Huang Guangtao , Wu Jun 
Published: November 2024

Abstract
Eukaryotic translation initiation factor 6 (eIF6) plays a crucial role in 60S ribosome biogenesis and protein translation, as well as in hypertrophic scar formation, but its potential role in epithelialization is still poorly understood. Herein, we found that eIF6 negatively correlated with the wound healing process. Mice with genetically knockdown eIF6 (eIF6+/−) showed faster re-epithelization as shown by the longer tongue of the newly formed epidermis. Furthermore, eIF6 ablation accelerated the wound healing process by targeting basal keratinocytes in the eIF6 keratinocyte-conditional knockout (eIF6f/+; Krt5-Cre+) mice. Mechanistically, keratin 6B, an important wound-activated protein, was significantly upregulated in eIF6f/+; Krt5-Cre+ mice skin as proved by RNA-seq, western immunoblots, and immunofluorescence staining. Moreover, an elevated level of KRT6B and accelerated proliferative capacity were also observed in stable knockdown eIF6 HaCaT cells. Taken together, eIF6 downregulation could accelerate epithelialization by upregulating KRT6B expression and promoting keratinocyte proliferation. Our results for the first time indicate that eIF6 might be a novel target to regulate re-epithelialization.
#PorkandPigs: an online media listening analysis of public perception of the U.S. swine industry
Authors: Fisk Alexandra E , Smith Michael L , Richert Brian T , Olynk Widmar Nicole J 
Published: November 2024

Abstract
With the majority of the U.S. swine industry being generally bounded by social licensing, there is a growing need to understand social perspectives to better adapt to consumer demands. Online and social media data are rich datasets that researchers are leveraging to tackle economic and societal challenges. The information that can be gleaned from online media regarding public perceptions makes it an important tool for producers to understand driving topics of interest to the public. This study utilized a prominent online and social media listening and data analytics platform to identify and quantify online and social media mentions related to the U.S. swine industry from January 1, 2020, to December 31, 2023. A general search for online media referencing pork and pigs was designed resulting in a total of 41,093,309 mentions. The search was further queried to specific topics of food and cooking (20,580,068 mentions), price (2,866,548 mentions), welfare (1,615,208 mentions), sustainability (771,819 mentions), and top authors/organizations within the U.S. swine industry (1,430 mentions). The study spanned the geographic locations of the overall US (including U.S. Minor Outlying Islands), the top 5 most populous states, and the top 5 pork-producing states as of 2024. Of all mentions, X/Twitter was the largest domain for nearly all mentions related to pork and pigs. Major organizations designed to spread information between the general public and pork producers had a minor impact on the overall conversation and no online media presence in the top 5 most populous states. Net sentiment was overall positive across all 4 yr and all geographies except for major events such as the COVID-19 pandemic, the H1N1 Swine Flu, the passing of California Proposition 12, environmental events, and inflation-associated pork prices. Overall, most media presence had positive net sentiments and with most interest surrounding ways to cook pork and how to keep bacon cheap. Understanding public perceptions of the U.S. swine industry provides the opportunity to make informed decisions on marketing strategies and production practices.
Young Black and White adults prefer in-person to telehealth for primary care visits and group health promotion programs
Authors: Gavrilova Lyubov , Watson Mellisa C , Eshera Yasmine M , Ridgel Angela L , Hughes Joel W 
Published: November 2024

Abstract
Telehealth utilization has increased since the coronavirus disease 2019 pandemic, reducing barriers to healthcare and, potentially, reducing participation in group health-promotion interventions. However, preferences for telehealth versus in-person formats have not been established. To examine preferences for telehealth and in-person format for primary care and group health-promotion interventions among Black and White women and men aged 20–39. We hypothesized that respondents would report a higher preference for telehealth than in-person appointments. This cross-sectional survey study recruited participants to answer questions about access to technology and preferences for telehealth and in-person formats of primary care and group health promotion. Respondents (n = 404) included similar proportions of White women (24.3%, 26.4 ± 4.3 years), Black women (25.0%, 29.0 ± 6.1 years), White men (25.9%, 32.8 ± 4.5 years), and Black men (24.8%, 30.6 ± 5.2 years). About 98.5% reported having a smartphone, and 80.4% had access to a computer with a camera. Preference ratings were higher for in-person visits, compared to telehealth visits, for both primary care (M = 3.86 ± 1.13 vs. M = 2.87 ± 1.18) and group health promotion (M = 3.72 ± 1.12 vs. M = 3.04 ± 1.20) F’s(1,400) &gt; 59.0, P’s &lt; .001. Most young adults have access to technology, supporting the feasibility of telehealth interventions. However, telehealth preference ratings were lower than in-person appointments. Preferences for delivery formats should be considered when designing behavioral interventions to promote health and prevent disease.
A unique compound ameliorating endoplasmic reticulum stress and insulin resistance by binding to β tubulin
Authors: Horiuchi Keigo , Matsushima Goshi , Wada Momoe , Thon Mina , Sobhy Mohamed Hossam , Ayoub Ahmed Taha , Hara Misato , Yoshii Michiko , Arakawa Kenji , Ozawa Koichiro , Hosoi Toru 
Published: November 2024

AbstractInsulin is secreted by the pancreatic β‐cells and regulates glucose uptake. Endoplasmic reticulum (ER) stress is known to induce insulin resistance. Identifying novel compounds, which can ameliorate ER stress and insulin resistance may be beneficial in the treatment of diabetes. Since treatment with compounds sourced from edible plants is relatively safe, this study aimed to identify a plant‐derived potential compound attenuating insulin resistance. In the present study, we identified apigenin as an effective compound for ameliorating ER stress and insulin resistance. It attenuated ER stress‐induced cell death and hepatic insulin resistance and improved abnormal glucose tolerance in a db/db diabetic model. The molecular mechanism of apigenin involved direct binding to β‐tubulin and improving tubulin stability, thereby recovering insulin resistance and developing diabetes. To our knowledge, no known antidiabetic drugs are yet known to target β‐tubulin. Edible plants containing apigenins, such as onions, oranges, and parsley, have been consumed since a long time. Therefore, the use of natural edible plants as a source may offer a safe strategy for the prevention of diabetes.
Hemin promotes platelet activation and plasma membrane disintegration regulated by the subtilisin‐like proprotein convertase furin
Authors: Schaale David , Laspa Zoi , Balmes Aylin , Sigle Manuel , Dicenta‐Baunach Valerie , Hochuli Ravi , Fu Xiaoqing , Serafimov Kristian , Castor Tatsiana , Harm Tobias , Müller Karin Anne Lydia , Rohlfing Anne‐Katrin , Laufer Stefan , Schäffer Tilman E. , Lämmerhofer Michael , Gawaz Meinrad 
Published: November 2024

AbstractPlatelet activation plays a critical role in thrombosis and hemostasis. Several pathophysiological situations lead to hemolysis, resulting in the liberation of free ferric iron‐containing hemin. Hemin has been shown to activate platelets and induce thrombo‐inflammation. Classical antiplatelet therapy failed to prevent hemin‐induced platelet activation. Thus, the aim of the present study was to characterize the mechanism of hemin‐induced platelet death (ferroptosis). We evaluated the in vitro effect of hemin on platelet activation, signaling, oxylipins, and plasma membrane destruction using light transmission aggregometry, ex vivo thrombus formation, multiparametric flow cytometry, micro‐UHPLC mass spectrometry for oxylipin profiling, and scanning ion conductance microscopy (SICM). We found that hemin induces platelet cell death indicated by increased ROS levels, phosphatidyl serine (PS) exposure, and loss of mitochondrial membrane potential (ΔΨm). Further, hemin causes lipid peroxidation and generation of distinct oxylipins, which strongly affects plasma membrane integrity leading to generation of platelet‐derived microvesicles. Interestingly, hemin‐dependent platelet death (ferroptosis) is specifically regulated by the subtilisin‐like proprotein convertase furin. In summary, platelet undergo a non‐apoptotic cell death mediated by furin. Inhibition of furin may offer a therapeutic strategy to control hemin‐induced thrombosis and thrombo‐inflammation at a site of hemolysis.
Prime editing: A gene precision editing tool from inception to present
Authors: Liu Zhihao , Guo Dong , Wang Dawei , Zhou Jinglin , Chen Qi , Lai Junzhong 
Published: November 2024

AbstractGenetic mutations significantly contribute to the onset of diseases, with over half of the cases caused by single‐nucleotide mutations. Advances in gene editing technologies have enabled precise editing and correction of mutated genes, offering effective treatment methods for genetic disorders. CRISPR/Cas9, despite its power, poses risks of inducing gene mutations due to DNA double‐strand breaks (DSB). The advent of base editing (BE) and prime editing (PE) has mitigated these risks by eliminating the hazards associated with DNA DSBs, allowing for more precise gene editing. This breakthrough lays a solid foundation for the clinical application of gene editing technologies. This review discusses the principles, development, and applications of PE gene editing technology in various genetic mutation‐induced diseases.
The whole‐body and skeletal muscle metabolic response to 14 days of highly controlled low energy availability in endurance‐trained females
Authors: Caldwell Hannah G. , Jeppesen Jan S. , Lossius Lone O. , Atti Jesper P. , Durrer Cody G. , Oxfeldt Mikkel , Melin Anna K. , Hansen Mette , Bangsbo Jens , Gliemann Lasse , Hellsten Ylva 
Published: November 2024

AbstractThis study investigated the effects of 14 days low energy availability (LEA) versus optimal energy availability (OEA) in endurance‐trained females on substrate utilization, insulin sensitivity, and skeletal muscle mitochondrial oxidative capacity; and the impact of metabolic changes on exercise performance. Twelve endurance‐trained females (V̇O2max 55.2 ± 5.1 mL × min−1 × kg−1) completed two 14‐day randomized, blinded, cross‐over, controlled dietary interventions: (1) OEA (51.9 ± 2.0 kcal × kg fat‐free mass (FFM)−1 × day−1) and (2) LEA (22.3 ± 1.5 kcal × kg FFM−1 × day−1), followed by 3 days OEA. Participants maintained their exercise training volume during both interventions (approx. 8 h × week−1 at 79% heart rate max). Skeletal muscle mitochondrial respiratory capacity, glycogen, and maximal activity of CS, HAD, and PFK were unaltered with LEA. 20‐min time trial endurance performance was impaired by 7.8% (Δ −16.8 W, 95% CI: −23.3 to −10.4, p < .001) which persisted following 3 days refueling post‐LEA (p < .001). Fat utilization was increased post‐LEA as evidenced by: (1) 99.4% (p < .001) increase in resting plasma free fatty acids (FFA); (2) 270% (p = .007) larger reduction in FFA in response to acute exercise; and (3) 28.2% (p = .015) increase in resting fat oxidation which persisted during submaximal exercise (p < .001). These responses were reversed with 3 days refueling. Daily glucose control (via CGM), HOMA‐IR, HOMA‐β, were unaffected by LEA. Skeletal muscle O2 utilization and carbohydrate availability were not limiting factors for aerobic exercise capacity and performance; therefore, whether LEA per se affects aspects of training quality/recovery requires investigation.
CD3D silencing alleviates diabetic nephropathy via inhibition of JAK/STAT pathway
Authors: Lei Xianghong , Zou Fangqin , Tang Xianhu , He Fengxia , Wang Jiyang , Cheng Shengyu , Lei Xiangxin 
Published: November 2024

AbstractDiabetic nephropathy (DN) is a severe microvascular complication of diabetes that poses a significant burden to global health. This investigation aims to illustrate the functional role of CD3D and its relevant mechanisms in DN progression. The pivotal genes between the GSE47183 and GSE30528 datasets were identified using bioinformatics methods. The effects of CD3D silencing on renal damage, inflammatory response, and lipid metabolism were validated in DN mice. Furthermore, the impacts of CD3D knockdown on cell viability, apoptotic rate, inflammation, and lipid levels were investigated in HK‐2 cells under high glucose (HG) conditions. Additionally, RO8191 was employed to investigate the role of CD3D in the JAK/STAT pathway in HG‐treated cells. A total of 5 focal genes were identified through bioinformatics and were found to be upregulated in renal tissues from DN mice. CD3D silencing mitigated pathological damage to kidneys, reduced inflammatory response, and decreased lipid accumulation in DN mice. HG stimulation restrained viability, increased apoptosis, promoted the release of inflammatory cytokines, and affected expressions of hallmarks related to lipid metabolism in HG‐treated cells; these changes were partially abolished by CD3D knockdown. Mechanistically, CD3D downregulation ameliorated HG‐induced injury in HK‐2 cells by blocking the JAK/STAT pathway. This study underscores that CD3D silencing has significant potential as a promising candidate in the treatment of DN.
EHD1 promotes breast cancer metastasis through upregulating HIF2a expression via activating mTOR pathway
Authors: Gao Xiaoqian , Li Juan , Feng Xuefei , Xie Yuchen , Zhang Juan , Liu Jie , Wang Bo , Liu Peijun 
Published: November 2024

AbstractThe multistep dynamic process of metastasis is the primary cause of breast cancer deaths. C‐terminal Eps15‐homology domain‐containing protein 1 (EHD1), a translocator associated with endocytic recycling, has been implicated in various oncogenic processes. However, the precise molecular mechanisms of EHD1‐induced breast cancer metastases remain largely unexplored. Here we found that the upregulation of EHD1 in breast cancer was positively associated with distant lymph node metastasis in patients. Meanwhile, EHD1 promoted epithelial‐mesenchymal transition (EMT), invasion, and metastasis of breast cancer cells in both two‐dimensional (2D) and three‐dimensional (3D) culture models in vitro, as well as in vivo. Remarkably, EHD1 can activate the AKT–mTOR pathway to upregulate the protein expression of hypoxia‐inducible factor 2α (HIF2α) under normoxic conditions and subsequently enhance the invasive and metastatic breast cancer. Our findings indicated EHD1 as a new regulator of HIF2α and a potential therapeutic target for inhibiting breast cancer metastasis.
Surgical Treatment Intensity at the End of Life in Patients With Cancer: A Systematic Review
Authors: Lawday Samuel , Zucker Benjamin E. , Gardner Shona , Robb James , Leandro Lorna , Hollingworth William , Blazeby Jane , McNair Angus G.K. , Chamberlain Charlotte 
Published: November 2024

Objective:
To synthesize evidence of surgical treatment intensity, defined as a measure of the quantity of invasive procedures, received by patients in patients with cancer within a defined time period around the ‘end of life’ (EoL).

Background:
Concern regarding overly ‘aggressive’ care or high health care utilization at the EoL, particularly in cancer, is growing. The contribution surgery makes to the quality and cost of EoL care in cancer has not yet been quantified.

Methods:
This PROSPERO registered systematic review used PRIMSA guidelines to search electronic databases for observational studies detailing surgical intensity at the EoL in adult cancer patients. Intensity was compared by disease, individual characteristics, geographical region, and palliative care involvement. A risk of bias tool assessed quality and a narrative synthesis of findings was completed.

Results:
In total, 39 papers were identified in this search. Up to 79% of patients underwent invasive procedures in the last month of life. Heterogeneity in patient groups, inclusion criteria, and EoL time periods lead to huge variation in results, with treatment intention often not identified. Patient, geographical, and pathological factors, alongside involvement of palliative/hospice care, were all identified as contributors to treatment intensity variation.

Conclusions:
A significant proportion of patients with cancer undergo invasive and costly invasive procedures at the EoL. There is significant reporting heterogeneity, with variation in patient inclusion criteria and EoL timeframes, demonstrating uncertainty within the literature. Identification of the context where surgical treatment intensity at the EoL is potentially inappropriate is not currently possible.
The Effects of a Stepped-Care Mental Health Program on Trauma Recidivism at a Level 1 Trauma Center
Authors: Rivas Gabriella A. , Gammel Juliette , George Kola , Ruggiero Kenneth J. , Davidson Tatiana , Espeleta Hannah C. , Hartsock Langdon , Reid Kristoff 
Published: November 2024

OBJECTIVES:
This study aimed to analyze the effects of a stepped-care mental health program (TRRP) on trauma recidivism at a single institution.

METHODS:

Designs:
Retrospective review.

Setting:
Single level 1 trauma center.

Patient Selection Criteria:
Trauma activation patients presenting at a single level 1 trauma center between 2017 and 2021 were selected. Data on demographics, mechanism of injury, readmissions, and TRRP participation were recorded.

Outcome Measures and Comparisons:
Main outcomes included recidivism rates, risk factors for representation, and determining whether a change in readmission risk existed after participation in TRRP.

RESULTS:
Intotal, 4484 patients were included, with a mean age of 44.8 years. 65.9% were men and 34.1% were women. Overall, 14.2% (637/4484) patients presenting to the same institution have experienced an unrelated traumatic incident after their index injury. Overall, 75.6% patients identified by TRRP participated in at least 1 TRRP service. Patients who did not participate in TRRP were statistically significantly more likely to be recidivists (15.6%) than those who did (10.9%, P < 0.001). Multivariable binary logistic regression analysis demonstrated statistically significant associations between increased trauma recidivism and being unmarried, Black/African American race, no use of seatbelts, alcohol intoxication at index injury, history of depression, alcohol use disorder, smoking, and government insurance (P ≤ 0.024). The multivariable regression model predicted a decrease in trauma recidivism by 33% in privately insured patients (P = 0.002), by 33% after participation in TRRP (P < 0.001), and by 18% after participation in each additional step of the TRRP program (P < 0.001).

CONCLUSIONS:
Results demonstrate reduced rates of trauma recidivism with involvement of the Trauma Resilience and Recovery Program. Although programs focused solely on psychosocial interventions may be sufficient to reduce the risk of subsequent traumatic events, further development of trauma rehabilitation programs should address both the psychosocial consequences of traumatic injuries and reinjury and readmission prevention.

LEVEL OF EVIDENCE:
Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
Efferocytosis: A new therapeutic target for stroke
Authors: Gao Li , Manaenko Anatol , Zeng Feng , Li Jingchen , Liu Lele , Xie Ruichuan , Zhang Xiaohua , Zhang John H. , Mei Qiyong , Tang Jiping , Hu Qin 
Published: November 2024

Abstract
Efferocytosis refers to the process that phagocytes recognize and remove the apoptotic cells, which is essential for maintaining tissue homeostasis both in physiological and pathological conditions. Numerous studies have demonstrated that efferocytosis can prevent secondary necrosis and proinflammatory factor release, leading to the resolution of inflammation and tissue immunological tolerance in numerous diseases such as stroke. Stroke is a leading cause of death and morbidity for adults worldwide. Persistent inflammation triggered by the dead cells or cell debris is a major contributor to post-stroke brain damage. Effective efferocytosis might be an efficient strategy to minimize inflammation and restore brain homeostasis for neuronal regeneration and function recovery. In this review, we will discuss the phagocytes in the brain, the molecular mechanisms underlying efferocytosis, the role of efferocytosis in inflammation resolution, and the potential therapeutic applications targeting efferocytosis in stroke.
Snapping of the Thumb Caused by Supernumerary Extensor Pollicis Longus Tendon
Authors: Oh Sangho , Son Daegu 
Published: November 2024

Summary:
Supernumerary extensor tendons of the hand are relatively common, whereas supernumerary extensor pollicis longus (EPL) tendons are rarely reported. Herein, we present the case of a 17-year-old boy with painful snapping at the anatomical snuffbox after repetitive pushups. Preoperative dynamic ultrasonography and magnetic resonance imaging revealed the bifurcation of EPL tendons. Through explorative surgery under wide-awake anesthesia, we found 2 EPL tendons with a single EPL muscle, which pass through the single extensor compartment and EPL canal distally. Intraoperatively, after releasing the extensor compartment and EPL canal, each EPL tendon was rerouted to a separate gliding plane to resolve the snapping symptom and recurrence. Six months postoperatively, the snapping symptom was resolved, and the patient resumed activities of daily living without any complications. This clinical case may be useful for examining anatomical variations of the thumb extensor tendon and detecting extraordinary pathological symptoms.