bluuebunny/crossref_metadata_2025_split huggingface dataset:
Late Quaternary (≥MIS 3 to MIS 1) stratigraphic transitions in a highland Beringian landscape along the Kuskokwim River, Alaska
Authors: Reuther Joshua D. , Rogers Jason , Druckenmiller Patrick , Bundtzen Thomas K. , Wallace Kristi , Bowman Robert , May Kevin , Feathers James , Cherkinsky Alexander 
Published: October 2019

AbstractStratigraphic records extending to Marine Oxygen Isotope Stage (MIS) 3 (57,000–29,000 cal yr BP) or older in Beringia are extremely rare. Three stratigraphic sections in interior western Alaska show near continuous sedimentological and environmental progressions extending from at least MIS 3, if not older, through MIS 1 (14,000 cal yr BP–present). The Kolmakof, Sue Creek, and VABM (vertical angle bench mark) Kuskokwim sections along the central Kuskokwim River, once a highland landscape at the fringe of central and eastern Beringia, contain aeolian deposition and soil sequences dating beyond 50,00014C yr BP. Thick peaty soil, shallow lacustrine, and tephra deposits represent the MIS 3 interstade (or older). Sand sheet and loess deposits, wedge cast development, and very thin soil development mark the later MIS 3 period and the transition into the MIS 2 stade (29,000–14,000 cal yr BP). Loess accumulation with thicker soil development occurred between ~16,000–13,500 cal yr BP at the MIS 2 and MIS 1 transition. After ~13,500 cal yr BP, loess accumulation waned and peat development increased throughout MIS 1. These stratigraphic sequences represent transitions between a warm and moist period during MIS 3, to a cooler and more arid period during MIS 2, then a return to warmer and moister climates in MIS 1.
Effects of alternative feed additives and flint maize grain particle size on growth performance, carcass traits and nutrient digestibility of finishing beef cattle
Authors: Gouvêa V. N. , Meschiatti M. A. P. , Moraes J. M. M. , Batalha C. D. A. , Dórea J. R. R. , Acedo T. S. , Tamassia L. F. M. , Owens F. N. , Santos F. A. P. 
Published: October 2019

AbstractThe current study evaluated growth performance and digestion responses of finishing bulls fed diets containing 825 g/kg flint maize [dry matter (DM) basis] ground to medium (1.66 mm; MG) or coarse particle sizes (2.12 mm; CG), with added monensin (26 mg/kg; DM basis; MON) or a blend of essential oils (BEO) + exogenousα-amylase (AM; 90 mg/kg + 560 mg/kg commercial product, respectively, DM basis). In Expt 1, 256 Nellore bulls were blocked by initial body weight (BW) (360 ± 11.7 kg) and assigned to 48 pens in a 2 × 2 factorial arrangement of treatments. Effect of a maize particle size × feed additive interaction was not detected for final BW, DM intake (DMI), average daily gain (ADG) and feed efficiency. The DMI was greater for bulls fed BEO + AMv.MON. Final BW and ADG tended to be greater for bulls fed CG than MG maize. An interaction was detected for hot carcass weight which was 11 kg heavier for bulls fed BEO + AMv.MON in diets containing CG, but not MG particle size. In Expt 2, four ruminally cannulated Nellore steers were offered the same treatments as Expt 1, in a 4 × 4 Latin Square design. Intake of most nutrients was greater for steers fed CG than steers fed MG maize. In summary, feeding bulls CG maize increased growth performance and carcass characteristics compared with MG. The combination of BEO + AM resulted in heavier carcass weights compared with MON supplementation when included in diets containing CG maize.
Generation of terahertz radiation from beating of two intense cosh-Gaussian laser beams in magnetized plasma
Authors: Purohit Gunjan , Rawat Vinod , Rawat Priyanka 
Published: October 2019

AbstractAn analytical and numerical study has been carried out for the generation of terahertz (THz) radiation by beating of two intense cosh-Gaussian laser beams (decentered Gaussian beams) in the rippled density magnetized plasma under the relativistic–ponderomotive regime. In this process, both laser beams exert a relativistic–ponderomotive force on plasma electrons at the beat frequency and impart them an oscillatory velocity in the presence of a static magnetic field. Due to coupling between this nonlinear oscillatory velocity with density ripple, nonlinear current is generated that excites the THz radiation at the different frequency. Higher-order paraxial-ray approximation (non-paraxial theory) has been used in this study. The effects of the decentered parameter, magnetic field, and density ripple on the THz radiation generation in ripple density magnetized plasma have been investigated. Further, the effect of beating of laser beams on the THz field amplitude and the efficiency of THz radiation have been studied. The amplitude and efficiency of the emitted radiation are found to be highly sensitive to the decentered parameter, magnetic field, and density ripple. It has been found that the amplitude and efficiency of the generated THz radiation increase significantly with increasing the values of decentered parameter, magnetic field, and density ripple.
Kinesthetic Guidance Utilizing DMP Synchronization and Assistive Virtual Fixtures for Progressive Automation
Authors: Papageorgiou Dimitrios , Dimeas Fotios , Kastritsi Theodora , Doulgeri Zoe 
Published: October 2019

SUMMARYThe progressive automation framework allows the seamless transition of a robot from kinesthetic guidance to autonomous operation mode during programming by demonstration of discrete motion tasks. This is achieved by the synergetic action of dynamic movement primitives (DMPs), virtual fixtures, and variable impedance control. The proposed DMPs encode the demonstrated trajectory and synchronize with the current demonstration from the user so that the reference generated motion follows the human’s demonstration. The proposed virtual fixtures assist the user in repeating the learned kinematic behavior but allow penetration so that the user can make modifications to the learned trajectory if needed. The tracking error in combination with the interaction forces and torques is used by a variable stiffness strategy to adjust the progressive automation level and transition the leading role between the human and the robot. An energy tank approach is utilized to apply the designed controller and to prove the passivity of the overall control method. An experimental evaluation of the proposed framework is presented for a pick and place task and results show that the transition to autonomous mode is achieved in few demonstrations.
Metronidazole therapy as initial treatment of Clostridium difficile infection in patients with chronic kidney disease in Korea
Authors: Shin Jaeuk , Wi Yu Mi , Lee Yu-Ji 
Published: October 2019

Abstract
The risk of metronidazole treatment failure in Clostridium difficile infection (CDI) patients with chronic kidney disease (CKD) or end-stage renal disease in Korea has not been established. We evaluated 481 patients who had been admitted to two secondary hospitals with a diagnosis of, and treatment for, CDI during 2010–2016. CDI patients were divided into three groups according to CKD status: non-CKD (n = 363), CKD (n = 55) and those requiring dialysis (n = 63). Logistic regression analyses were performed to examine the association of CKD status with treatment failure. CDI patients receiving dialysis tended to have increased odds of metronidazole and overall treatment failure compared to non-CKD patients; adjusted odds ratios and 95% confidence intervals were 2.09 (1.03–4.21) and 2.18 (1.11–4.32) for metronidazole and overall treatment failure, respectively. However, CKD patients did not have increased odds of metronidazole or overall treatment failure compared to non-CKD patients, even where severe CDI was more prevalent in CKD patients. The incidence of symptomatic ileus or toxic megacolon did not differ among groups. Our results suggest that initial metronidazole therapy may be considered in CDI patients with non-dialysis CKD, but should not be considered in CDI patients undergoing dialysis.
Better diet quality scores are associated with a lower risk of hypertension and non-fatal CVD in middle-aged Australian women over 15 years of follow-up
Authors: Jackson Jacklyn K , MacDonald-Wicks Lesley K , McEvoy Mark A , Forder Peta M , Holder Carl , Oldmeadow Christopher , Byles Julie E , Patterson Amanda J 
Published: October 2019

AbstractObjective:To explore if better diet quality scores as a measure of adherence to the Australian Dietary Guidelines (ADG) and the Mediterranean diet (MedDiet) are associated with a lower incidence of hypertension and non-fatal CVD.Design:Prospective analysis of the 1946–1951 cohort of the Australian Longitudinal Study on Women’s Health (ALSWH). The Australian Recommended Foods Score (ARFS) was calculated as an indicator of adherence to the ADG; the Mediterranean Diet Score (MDS) measured adherence to the MedDiet. Outcomes included hypertension and non-fatal CVD. Generalised estimating equations estimated OR and 95 % CI across quartiles of diet quality scores.Setting:Australia, 2001–2016.Participants:1946–1951 cohort of the ALSWH (n 5324), without CVD, hypertension and diabetes at baseline (2001), with complete FFQ data.Results:There were 1342 new cases of hypertension and 629 new cases of non-fatal CVD over 15 years of follow-up. Multivariate analysis indicated that women reporting better adherence to the ARFS (≥38/74) had 15 % (95 % CI 1, 28 %;P= 0·05) lower odds of hypertension and 46 % (95 % CI 6, 66 %;P= 0·1) lower odds of non-fatal CVD. Women reporting better adherence to the MDS (≥8/17) had 27 % (95 % CI 15, 47 %;P= 0·0006) lower odds of hypertension and 30 % (95 % CI 2, 50 %;P= 0·03) lower odds of non-fatal CVD.Conclusions:Better adherence to diet quality scores is associated with lower risk of hypertension and non-fatal CVD. These results support the need for updated evidenced based on the ADG as well as public health nutrition policies in Australia.
SOCIALLY DESIRABLE REPORTING AND THE EXPRESSION OF BIOLOGICAL CONCEPTS OF RACE
Authors: Morning Ann , Brückner Hannah , Nelson Alondra 
Published: October 2019

AbstractIn recent decades, dramatic developments in genetics research have begun to transform not only the practice of medicine but also conceptions of the social world. In the media, in popular culture, and in everyday conversation, Americans routinely link genetics to individual behavior and social outcomes. At the same time, some social researchers contend that biological definitions of race have lost ground in the United States over the last fifty years. At the crossroads of two trends—on one hand, the post-World War II recoil from biological accounts of racial difference, and on the other, the growing admiration for the advances of genetic science—the American public’s conception of race is a phenomenon that merits greater attention from sociologists than it has received to date. However, survey data on racial attitudes has proven to be significantly affected by social desirability bias. While a number of studies have attempted to measure social desirability bias with regard to racial attitudes, most have focused on racial policy preferences rather than genetic accounts of racial inequality. We employ a list experiment to create an unobtrusive measure of support for a biologistic understanding of racial inequality. We show that one in five non-Black Americans attribute income inequality between Black and White people to unspecified genetic differences between the two groups. We also find that this number is substantially underestimated when using a direct question. The magnitude of social desirability effects varies, and is most pronounced among women, older people, and the highly-educated.
Avera Twin Register Growing Through Online Consenting and Survey Collection
Authors: Kittelsrud Julie M. , Ehli Erik A. , Petersen Vikki , Jung Tammy , Beck Jeffrey J. , Kallsen Noah , Huizenga Patricia , Holm Brittany , Davies Gareth E. 
Published: October 2019

AbstractThe aim of the Avera Twin Register (ATR) is to establish a prospective longitudinal repository of twins, multiples, siblings and family members’ biological samples to study environmental and genetic influences on health and disease. Also, it is our intention to contribute to international genome-wide association study (GWAS) twin consortia when appropriate sample size is achieved within the ATR. The ATR is young compared with existing registers and continues to collect a longitudinal repository of biological specimens, survey data and health information. Data and biological specimens were originally collected via face-to-face appointments or the postal department and consisted of paper-informed consents and questionnaires. Enrollment of the ATR began on May 18, 2016 and is located in Sioux Falls, South Dakota, a rural and frontier area in the Central United States with a regional population of approximately 880,000. The original target area for the ATR was South Dakota and the four surrounding states: Minnesota, Iowa, North Dakota and Nebraska. The ATR has found a need to expand that area based on twin and multiple siblings who live in various areas surrounding these states. A description of the state of the ATR today and its transition to online data collection and informed consent will be presented. The ATR collects longitudinal data on lifestyle, including diet and activity levels, aging, plus complex traits and diseases. All twins and multiples participating in the ATR are genotyped on the Illumina Global Screening Array and receive zygosity results.
The New West Japan Twins and Higher Order Multiple Births Registry
Authors: Yokoyama Yoshie 
Published: October 2019

AbstractThe new West Japan Twins and Higher Order Multiple Births Registry was established by recruiting young twins and multiple births and by referrals from public health centers in the 1990s. The participants included in the survey comprised over 7800 twins and 4241 higher order multiples, and their families. Specifically, the present registry contains one of the largest triplet samples in the world. For these twins and multiples, data on year of delivery, mode of delivery, gestational age, intrapartum complications, longitudinal physical measures, motor milestones, cerebral palsy and feeding methods were obtained from records in the Maternal and Child Health Handbooks and schools. Participating mothers were asked to indicate family structure, parental educational history, maternal sleeping time, maternal health condition, maternal and paternal age at multiple delivery, complications during pregnancy, handedness of multiples and age at menarche of multiples. However, the zygosity differed among the various collaborating public health centers according to factors such as the time of investigation. Follow-up questionnaires have been mailed out every 3–4 years for longitudinal studies. This article describes the goals of this registry, recruitment of multiples and the focus of the study. The goals of this registry are not only to conduct research on human genetics and maternal and child health, but also to contribute to providing appropriate information for families with multiples.
CCL2 promotes macrophages-associated chemoresistance via MCPIP1 dual catalytic activities in multiple myeloma
Authors: Xu Ruyi , Li Yi , Yan Haimeng , Zhang Enfan , Huang Xi , Chen Qingxiao , Chen Jing , Qu Jianwei , Liu Yang , He Jingsong , Yi Qing , Cai Zhen 
Published: October 2019

AbstractWe previously showed that the chemokine CCL2 can recruit macrophages (Mφs) to the bone marrow (BM) in multiple myeloma (MM) and that myeloma-associated Mφs are important in drug resistance. Here, we explore the role of increased CCL2 expression in the BM microenvironment of MM and elucidate the underlying mechanism. Our results show that CCL2 expression is associated with the treatment status of MM patients. Mφs interact with MM cells and further upregulate their expression of CCL2. These increased level of CCL2 polarizes Mφs toward the M2-like phenotype and promotes Mφs to protect MM cells from drug-induced apoptosis. Mechanistically, CCL2 upregulated the expression of the immunosuppressive molecular MCP-1-induced protein (MCPIP1) in Mφs. MCPIP1 mediates Mφs’ polarization and protection via dual catalytic activities. Additionally, we found that CCL2 induces MCPIP1 expression via the JAK2-STAT3 signaling pathway. Taken together, our results indicate that increased CCL2 expression in MM patients’ BM polarizes Mφs toward the M2-like phenotype and promotes the protective effect of Mφs through MCPIP1, providing novel insight into the mechanism of Mφs-mediated drug resistance in MM.
Transduction optimization of AAV vectors for human gene therapy of glaucoma and their reversed cell entry characteristics
Authors: Rodriguez-Estevez Laura , Asokan Priyadarsini , Borrás Teresa 
Published: October 2019

Abstract
The trabecular meshwork (TM) of the eye is responsible for maintaining physiological intraocular pressure (IOP). Dysfunction of this tissue results in elevated IOP, subsequent optic nerve damage and glaucoma, the world’s leading cause of irreversible blindness. IOP regulation by delivering candidate TM genes would offer an enormous clinical advantage to the current daily-drops/surgery treatment. Initially, we showed that a double-stranded AAV2 (scAAV2) transduced the human TM very efficiently, while its single-stranded form (ssAAV2) did not. Here, we quantified transduction and entry of single- and double-strand serotypes 1, 2.5, 5, 6, 8, and 9 in primary, single individual-derived human TM cells (HTM). scAAV2 exhibited highest transduction in all individuals, distantly followed by scAAV2.5, scAAV6, and scAAV5. Transduction of scAAV1, scAAV8, and scAAV9 was negligible. None of the ssAAV serotypes transduced, but their cell entries were significantly higher than those of their corresponding scAAV. Tyrosine scAAV2 capsid mutants increased transduction in HTM cultured cells and all TM-outflow layers of perfused postmortem human eyes. These studies provide the first serotype optimization for gene therapy of glaucoma in humans. They further reveal biological differences between the AAV forms in HTM cells, whose understanding could contribute to the development of gene therapy of glaucoma.
Comparative proteomic analysis of cucumber powdery mildew resistance between a single-segment substitution line and its recurrent parent
Authors: Xu Xuewen , Liu Xueli , Yan Yali , Wang Wei , Gebretsadik Kiros , Qi Xiaohua , Xu Qiang , Chen Xuehao 
Published: October 2019

Abstract
Powdery mildew (PM) is considered a major cause of yield losses and reduced quality in cucumber worldwide, but the molecular basis of PM resistance remains poorly understood. A segment substitution line, namely, SSL508-28, was developed with dominant PM resistance in the genetic background of PM-susceptible cucumber inbred line D8. The substituted segment contains 860 genes. An iTRAQ-based comparative proteomic technology was used to map the proteomes of PM-inoculated and untreated (control) D8 and SSL508-28. The number of differentially regulated proteins (DRPs) in SSL508-28 was almost three times higher than that in D8. Fourteen DRPs were located in the substituted segment interval. Comparative gene expression analysis revealed that nodulin-related protein 1 (NRP1) may be a good candidate for PM resistance. Gene Ontology enrichment analysis showed that DRPs functioning in tetrapyrrole biosynthetic process, sulfur metabolic process and cell redox homeostasis were specifically enriched in the resistant line SSL508-28. DRPs categorized in the KEGG term photosynthesis increased in both lines upon PM infection, suggesting that the strategies used by cucumber may be different from those used by other crops to react to PM attacks at the initial stage. The measurement of hydrogen peroxide and superoxide anion production and net photosynthetic rate were consistent with the changes in protein abundance, suggesting that the proteomic results were reliable. There was a poor correlation between DRPs measured by iTRAQ and the corresponding gene expression changes measured by RNA-seq with the same experimental design. Taken together, these findings improve the understanding of the molecular mechanisms underlying the response of cucumber to PM infection.
Genetic and genomic resources for Rubus breeding: a roadmap for the future
Authors: Foster Toshi M. , Bassil Nahla V. , Dossett Michael , Leigh Worthington Margaret , Graham Julie 
Published: October 2019

AbstractRubusfruits are high-value crops that are sought after by consumers for their flavor, visual appeal, and health benefits. To meet this demand, production of red and black raspberries (R. idaeusL. andR. occidentalisL.), blackberries (R. subgenusRubus), and hybrids, such as Boysenberry and marionberry, is growing worldwide.Rubusbreeding programmes are continually striving to improve flavor, texture, machine harvestability, and yield, provide pest and disease resistance, improve storage and processing properties, and optimize fruits and plants for different production and harvest systems. Breeders face numerous challenges, such as polyploidy, the lack of genetic diversity in many of the elite cultivars, and until recently, the relative shortage of genetic and genomic resources available forRubus. This review will highlight the development of continually improving genetic maps, the identification of Quantitative Trait Loci (QTL)s controlling key traits, draft genomes for red and black raspberry, and efforts to improve gene models. The development of genetic maps and markers, the molecular characterization of wild species and germplasm, and high-throughput genotyping platforms will expedite breeding of improved cultivars. Fully sequenced genomes and accurate gene models facilitate identification of genes underlying traits of interest and enable gene editing technologies such as CRISPR/Cas9.
Harmonization of Zika neutralization assays by using the WHO International Standard for anti-Zika virus antibody
Authors: Mattiuzzo Giada , Knezevic Ivana , Hassall Mark , Ashall James , Myhill Sophie , Faulkner Valwynne , Hockley Jason , Rigsby Peter , Wilkinson Dianna E. , Page Mark ,   the collaborative study participants, Donolato Marco , Baylis Sally , Yue Constanza , Elgner Fabian , Yoon In-Kyu , Yang Jae Seung , Song Manki , Gonzalez-Escobar Gabriel , Brindle Richard , Tedder Richard , Dicks Steve , Ushiro-Lumb Ines , Williams-McDonald Sarah , Kumar Sanjai , Peden Keith , Golding Hana , Khurana Surender , Bonaparte Matthew , Durbin Anna , Dean Hansi , Sonnberg Stephanie , Hogrefe Wayne , Crowe James , Voss Thomas , Collins Matthew , Pierson Theodore , Burgomaster Katherine , Dowd Kimberly , Sigismondi Louise , Gunasekera Dhammika , Wassenberg James , Eckels Kenneth , De La Barrera Rafael 
Published: October 2019

AbstractDuring outbreaks of emerging viruses, such as the Zika outbreak in 2015–2016, speed and accuracy in detection of infection are critical factors to control the spread of the disease; often serological and diagnostic methods for emerging viruses are not well developed and validated. Thus, vaccines and treatments are difficult to evaluate due to the lack of comparable methods. In this study, we show how the 1st WHO International Standard for anti-Zika antibody was able to harmonize the neutralization titres of a panel of serological Zika-positive samples from laboratories worldwide. Expression of the titres in International Unit per millilitre reduced the inter-laboratory variance, allowing for greater comparability between laboratories. We advocate the use of the International Standard for anti-Zika virus antibodies for the calibration of neutralization assays to create a common language, which will permit a clear evaluation of the results of different clinical trials and expedite the vaccine/treatment development.