bluuebunny/crossref_metadata_2025_split huggingface dataset:
Normal development and fertility of Fut1, Fut2, and Sec1 triple knockout mice
Authors: Chen Jiaxi , Su Zhipeng , Zhang Chunlei , Li Fenge , Hwu Patrick , Wang Zhen , Wang Yanping , Li Yunsen , Tong Jiao , Chen Chunchao , Zhou Dapeng 
Published: April 2019

AbstractThe fucose alpha(1,2) galactose structure (H antigen) is synthesized by α1,2 fucosyltransferases Fut1, Fut2 and Sec1. H antigen has been reported to be involved in cancer progression, neurite migration, synaptic plasticity, host-microbe interaction, blastocyst implantation, and the maintenance of gut microbiome. Genetic depletion of Fut1 or Fut2 only cause defects of alpha1,2 fucosylation in limited tissues because of enzyme redundancy. In this study, we generated mice with deficiencies in Fut1, Fut2, and Sec1 genes to deplete H antigen through BAC Engineering for the generation of ES Cell-Targeting construct. The homogenous triple knockout mice showed no significant decrease of viability or development. Mass spectrometry and Western blot analysis confirmed the absence of H blood group antigen in multiple organs. These results indicate normal development and fertility of mice devoid of blood group H. The fine pathophysiological alterations in these mice remain to be carefully studied, and they may serve as valuable tools to study gut microbiome and host-microbe interactions.
Intertwined trade-offs coordinateDrosophilamidgut mitosis vs. endoreplication and host defense vs. dysplasia
Authors: Tamamouna Vasilia , Panagi Myrofora , Theophanous Andria , Demosthenous Maria , Michail Maria , Papadopoulou Markella , Teloni Savvas , Pitsouli Chrysoula , Apidianakis Yiorgos 
Published: April 2019

AbstractInflammatory signaling supports host defense against infection, not only through immune cells, but also via regeneration of damaged tissue. Heightened regeneration, nevertheless, predisposes for all types of cancer and thus a trade-off exists between regeneration capacity and long-term tissue homeostasis. Here, we study the role of tissue-intrinsic regenerative inflammatory signaling in stem cell mitosis of the adultDrosophilamidgut at the baseline and the infected state and its impact on intestinal host defense to infection and stem cell-mediated dysplasia. Through a quantitative genetics screen we find that stem cell mitosis is positively linked with the expression ofeiger, Delta, upd3andveinin the midgut, as well as with dysplasia and host defense, but negatively with enterocyte endoreplication. We provide evidence that intertwined trade-offs fine-tune midgut homeostasis, according to which stem cell mitosis throughcyclin Ein stem cells promotes the optimal host defense to infection, unless dysplasia ensues. However,cyclin Ein enteroblasts promotes enterocyte endoreplication and counterbalances stem cell mitosis and dysplasia, providing an alternative but less efficient mechanism to support host defense.
UNC-45A is preferentially expressed in epithelial cells and binds to and co-localizes with interphase MTs
Authors: Habicht Juri , Mooneyham Ashley , Shetty Mihir , Zhang Xiaonan , Shridhar Vijayalakshmi , Winterhoff Boris , Zhang Ying , Cepela Jason , Starr Timothy , Lou Emil , Bazzaro Martina 
Published: April 2019

AbstractUNC-45A is a ubiquitously expressed protein highly conserved throughout evolution. Most of what we currently know about UNC-45A pertains to its role as a regulator of the actomyosin system. However, emerging studies from both our and other laboratories support a role of UNC-45A outside of actomyosin regulation. This includes studies showing that UNC-45A: regulates gene transcription, co-localizes and biochemically co-fractionates with gamma tubulin and regulates centrosomal positioning, is found in the same subcellular fractions where MT-associated proteins are, and is a mitotic spindle-associated protein with MT destabilizing activity in absence of the actomyosin system.Here, we extended our previous findings and show that UNC45A is variably expressed across a spectrum of cell lines with the highest level being found in HeLa cells and in ovarian cancer cells inherently paclitaxel-resistant. Furthermore, we show that UNC-45A is preferentially expressed in epithelial cells, localizes to mitotic spindles in clinical tumor specimens of cancer and co-localizes and co-fractionates with MTs in interphase cells independent of actin or myosin.In sum, we report alteration of UNC45A localization in the setting of chemotherapeutic treatment of cells with paclitaxel, and localization of UNC45A to MTs both in vitro and in vivo. These findings will be important to ongoing and future studies in the field that further identify the important role of UNC45A in cancer and other cellular processes.
A rapid and accurate MinION-based workflow for tracking species biodiversity in the field
Authors: Maestri Simone , Cosentino Emanuela , Paterno Marta , Freitag Hendrik , Garces Jhoana M. , Marcolungo Luca , Alfano Massimiliano , Njunjić Iva , Schilthuizen Menno , Slik Ferry , Menegon Michele , Rossato Marzia , Delledonne Massimo 
Published: April 2019

AbstractGenetic markers (DNA barcodes) are often used to support and confirm species identification. Barcode sequences can be generated in the field using portable systems based on the Oxford Nanopore Technologies (ONT) MinION platform. However, to achieve a broader application, current proof-of-principle workflows for on-site barcoding analysis must be standardized to ensure reliable and robust performance under suboptimal field conditions without increasing costs. Here we demonstrate the implementation of a new on-site workflow for DNA extraction, PCR-based barcoding and the generation of consensus sequences. The portable laboratory features inexpensive instruments that can be carried as hand luggage and uses standard molecular biology protocols and reagents that tolerate adverse environmental conditions. Barcodes are sequenced using MinION technology and analyzed with ONTrack, an original de novo assembly pipeline that requires as few as 500 reads per sample. ONTrack-derived consensus barcodes have high accuracy, ranging from 99,8% to 100%, despite the presence of homopolymer runs. The ONTrack pipeline has a user-friendly interface and returns consensus sequences in minutes. The remarkable accuracy and low computational demand of the ONTrack pipeline, together with the inexpensive equipment and simple protocols, make the proposed workflow particularly suitable for tracking species under field conditions.
Exploring the role of risk and corruption on bank stability: evidence from Pakistan
Authors: Ali Muhammad , Sohail Amna , Khan Lubna , Puah Chin-Hong 
Published: April 2019

Purpose
This paper aims to explore the impact of liquidity risk, credit risk, funding risk and corruption on bank stability of the banking system in Pakistan.

Design/methodology/approach
The empirical analysis is confined to 24 retail banks, which include 5 Islamic and 19 conventional banks during the period of 2007-2015.

Findings
The findings of this study suggest that bank size, liquidity risk, funding risk and corruption exert a positive impact on bank stability. Additionally, the authors find a negative relationship between credit risk and bank stability.

Originality/value
As per the knowledge of the authors, the present research is the first attempt that discusses the issues of bank stability related to risk and corruption faced by the banking system.
Sustaining open innovation through a “Center of Excellence”
Authors: Richard Elizabeth E. , Davis Jeffrey R. , Paik Jin H. , Lakhani Karim R. 
Published: April 2019

Purpose
This paper presents NASA’s experience using a Center of Excellence (CoE) to scale and sustain an open innovation program as an effective problem-solving tool and includes strategic management recommendations for other organizations based on lessons learned.

Design/methodology/approach
This paper defines four phases of implementing an open innovation program: Learn, Pilot, Scale and Sustain. It provides guidance on the time required for each phase and recommendations for how to utilize a CoE to succeed. Recommendations are based upon the experience of NASA’s Human Health and Performance Directorate, and experience at the Laboratory for Innovation Science at Harvard running hundreds of challenges with research and development organizations.

Findings
Lessons learned include the importance of grounding innovation initiatives in the business strategy, assessing the portfolio of work to select problems most amenable to solving via crowdsourcing methodology, framing problems that external parties can solve, thinking strategically about early wins, selecting the right platforms, developing criteria for evaluation, and advancing a culture of innovation. Establishing a CoE provides an effective infrastructure to address both technical and cultural issues.

Originality/value
The NASA experience spanned more than seven years from initial learnings about open innovation concepts to the successful scaling and sustaining of an open innovation program; this paper provides recommendations on how to decrease this timeline to three years.
Case of severe bullous erythema including intertrigo‐like eruptions with angioedema induced by pegylated liposomal doxorubicin
Authors: Totsuka Michiru , Watanabe Yuko , Asai Chika , Takahashi Saki , Ishikawa Hideyuki , Takamura Naoko , Hagiwara Mayumi , Aihara Michiko 
Published: April 2019

AbstractPegylated liposomal doxorubicin (PLD) is an anthracycline anticancer agent used in ovarian cancer and a form of doxorubicin enclosed in pegylated liposomes. There are only a few reports on intertrigo‐like eruptions caused by PLD. We describe the first case of severe bullous erythema, including intertrigo‐like eruptions with angioedema, induced by PLD in Japan. We present the case of a 53‐year‐old woman who was diagnosed with stage IIIC ovarian cancer. After receiving three cycles of PLD, the patient developed swelling of the upper lip and painful erythema with blisters and erosions on the axilla, upper back, flank and wrists. The patient was diagnosed with angioedema and severe skin lesions, including intertrigo‐like eruptions induced by PLD. Although treatment with oral prednisolone and topical steroids was effective against these eruptions, the administration of PLD was discontinued because of its ineffectiveness against the primary disease. Several risk factors, such as obesity, perspiration and racial differences, may contribute toward a severe manifestation such as that seen in our patient. Moreover, our case was the first accompanied by angioedema. The mechanism of coexistence of intertrigo‐like eruptions and angioedema is not clear; further studies are required to clarify the pathological mechanism of intertrigo‐like eruptions.
Effects of stand age, richness and density on productivity in subtropical forests in China
Authors: Ouyang Shuai , Xiang Wenhua , Wang Xiangping , Xiao Wenfa , Chen Liang , Li Shenggong , Sun Han , Deng Xiangwen , Forrester David I. , Zeng Lixiong , Lei Pifeng , Lei Xiangdong , Gou Mengmeng , Peng Changhui 
Published: April 2019

Abstract

Forest productivity may be determined not only by biodiversity but also by environmental factors and stand structure attributes. However, the relative importance of these factors in determining productivity is still controversial for subtropical forests.

Based on a large dataset from 600 permanent forest inventory plots across subtropical China, we examined the relationship between biodiversity and forest productivity and tested whether stand structural attributes (stand density in terms of trees per ha, age and tree size) and environmental factors (climate and site conditions) had larger effects on productivity. Furthermore, we quantified the relative importance of environmental factors, stand structure and diversity in determining forest productivity.

Diversity, together with stand structure and site conditions, regulated the variability in forest productivity. The relationship between diversity and forest productivity did not vary along environmental gradients. Stand density and age were more important modulators of forest productivity than diversity.

Synthesis. Diversity had significant and positive effects on productivity in species‐rich subtropical forests, but the effects of stand density and age were also important. Our work highlights that while biodiversity conservation is often important, the regulation of stand structure can be even more important to maintain high productivity in subtropical forests.
Specific class of intrapartum antibiotics relates to maturation of the infant gut microbiota: a prospective cohort study
Authors: Coker MO , Hoen AG , Dade E , Lundgren S , Li Z , Wong AD , Zens MS , Palys TJ , Morrison HG , Sogin ML , Baker ER , Karagas MR , Madan JC 
Published: April 2019

ObjectiveTo evaluate the potential impact of intrapartum antibiotics, and their specific classes, on the infant gut microbiota in the first year of life.DesignProspective study of infants in the New Hampshire Birth Cohort Study (NHBCS).SettingsRural New Hampshire, USA.Population or sampleTwo hundred and sixty‐six full‐term infants from the NHBCS.MethodsIntrapartum antibiotic use during labour and delivery was abstracted from medical records. Faecal samples collected at 6 weeks and 1 year of age were characterised by 16S rRNA sequencing, and metagenomics analysis in a subset of samples.ExposuresMaternal exposure to antibiotics during labour and delivery.Main outcome measureTaxonomic and functional profiles of faecal samples.ResultsInfant exposure to intrapartum antibiotics, particularly to two or more antibiotic classes, was independently associated with lower microbial diversity scores as well as a unique bacterial community at 6 weeks (GUnifrac, P = 0.02). At 1 year, infants in the penicillin‐only group had significantly lower α diversity scores than infants not exposed to intrapartum antibiotics. Within the first year of life, intrapartum exposure to penicillins was related to a significantly lower increase in several taxa including Bacteroides, use of cephalosporins was associated with a significantly lower rise over time in Bifidobacterium and infants in the multi‐class group experienced a significantly higher increase in Veillonella dispar.ConclusionsOur findings suggest that intrapartum antibiotics alter the developmental trajectory of the infant gut microbiome, and specific antibiotic types may impact community composition, diversity and keystone immune training taxa.Tweetable abstractClass of intrapartum antibiotics administered during delivery relates to maturation of infant gut microbiota.
Clomiphene citrate and neural tube defects: a meta‐analysis of controlled observational studies
Authors: Auffret M , Cottin J , Vial T , Cucherat M 
Published: April 2019

BackgroundAt the end of the 1980s, several studies suggested a potential increased risk of neural tube defects (NTDs) with ovulation induction/fertility drugs, especially with clomiphene citrate (CC). A previous meta‐analysis of observational studies evaluating the risk of NTDs associated with the use of CC performed in 1995 found a risk ratio of 1.08 (95% CI 0.76–1.51). Since then, additional studies have been published and the risk of NTDs associated with periconceptional CC exposure may have changed.ObjectiveTo perform an updated quantitative meta‐analysis of the risk of NTDs associated with periconceptional CC exposure.Search strategyMEDLINE, Web of Science, and Scopus were searched (October 2018).Selection criteriaComparative cohort and case–control studies investigating the risk of NTDs after periconceptional CC exposure.Data collection and analysisPooled effect sizes with corresponding 95% CIs were calculated using random effects models, comparing the risk of NTDs between pregnancies exposed and not exposed to CC.Main resultsThirteen studies met the inclusion criteria, totalling 218 819 pregnancies. Periconceptional exposure to CC was not significantly associated with an increased risk of NTDs (pooled odds ratio 1.21, 95% CI 0.88–1.66). No heterogeneity between studies was observed (I2 = 26%). A funnel plot and asymmetry test were not suggestive of publication bias.ConclusionOur meta‐analysis confirms that exposure to CC before or in early pregnancy was associated with a 21% increased risk of NTD in relation to CC exposure; however, this increased risk is not statistically significant.Tweetable abstractA new meta‐analysis finds that clomiphene citrate exposure before or in early pregnancy is not associated with an increased risk of NTDs.
Reimagining Sutherland 80 years after white‐collar crime*
Authors: Simpson Sally S. 
Published: April 2019

AbstractEighty years ago, Edwin H. Sutherland conceptualized and defined white‐collar crime. In this article, I engage retrospectively with Sutherland's ideas and work to emphasize important aspects that continue to guide research today; to note where he was prescient as well as shortsighted. I center this discussion around “corporate crime” or crimes by business. Four main themes are discussed: 1) law and official responses to corporate offending—the data problem, 2) corporate crime and the life cycle of organizations, 3) psychological and trait‐based explanations, and 4) consequences of definitional ambiguity.
Innovative microscale workflow from fungi cultures to Cell Wall‐Degrading Enzyme screening
Authors: Raulo Roxane , Heuson Egon , Siah Ali , Phalip Vincent , Froidevaux Renato 
Published: April 2019

SummaryThis study aimed at developing a complete miniaturized high‐throughput screening workflow for the evaluation of the Cell Wall‐Degrading Enzyme (CWDE) activities produced by any fungal strain directly cultivated on raw feedstock in a submerged manner. In this study, wheat straw was selected as model substrate as it represents an important carbon source but yet poorly valorised to yield high added value products. Fungi were grown in a microbioreactor in a high‐throughput (HT) way to replace the fastidious shaking flask cultivations. Both approaches were compared in order to validate our new methodology. The range of CWDE activities produced from the cultures was assayed using AZO‐died and pNP‐linked substrates in an SBS plate format using a Biomek FXp pipetting platform. As highlighted in this study, it was shown that the CWDE activities gathered from the microbioreactor cultivations were similar or higher to those obtained from shake flasks cultures, with a lower standard deviation on the measured values, making this new method much faster than the traditional one and suitable for HT CWDE production thanks to its pipetting platform compatibility. Also, the results showed that the enzymatic activities measured were the same when doing the assay manually or using the automated method.
Clinical analysis of EGFR‐positive non‐small cell lung cancer patients treated with first‐line afatinib: A Nagano Lung Cancer Research Group
Authors: Sonehara Kei , Kobayashi Takashi , Tateishi Kazunari , Morozumi Nobutoshi , Yoshiike Fumiaki , Hachiya Tsutomu , Ono Yasushi , Takasuna Keiichirou , Agatsuma Toshihiko , Masubuchi Takeshi , Matsuo Akemi , Tanaka Hozumi , Morikawa Akio , Hanaoka Masayuki , Koizumi Tomonobu 
Published: April 2019

BackgroundIn the LUX‐Lung 3 and LUX‐Lung 6 trials, afatinib improved overall survival in previously untreated patients with EGFR 19del mutated non‐small cell lung cancer (NSCLC) compared to chemotherapy. The appropriate management of adverse events and dose reduction of afatinib are important for EGFR‐positive NSCLC patients. We conducted a retrospective and observational study of patients treated with first‐line afatinib for EGFR‐positive NSCLC in Nagano prefecture, Japan, focusing on efficacy and toxicities.MethodsWe retrospectively collected the medical records of NSCLC patients initially treated with afatinib between May 2014 and March 2018.ResultsA total of 62 patients with a median age of 67 years and a median body surface area (BSA) of 1.57 m2 were included. The overall response rate was 87.7% and median progression‐free survival (PFS) was 15.7 months. The median PFS was similar between standard initial dose (40 mg) and reduced initial doses (30 and 20 mg) (15.7 vs. 14.2 months; P = 0.978). The frequency of dose reduction and the discontinuation rate in the 40 mg daily dose group was higher in patients with BSA < 1.58 m2 (100%) compared to BSA ≥ 1.58 m2 (68.2%) (P = 0.014). The frequency of diarrhea was higher in patients with BSA < 1.58 m2 (93.5%) compared to BSA ≥ 1.58 m2 (71.0%) (P = 0.02).ConclusionIn real‐world clinical practice, first‐line afatinib was well managed and was equally as effective as in previous clinical trials of EGFR‐positive NSCLC. BSA is considered a predictive marker for appropriate afatinib dose reduction.
Chronic epididymitis and leptin and their associations with semen characteristics in men with infertility
Authors: Xin Song , Hao Ye , Zhi‐Peng Ma , Nanhe Lin , Bin Chen 
Published: April 2019

AbstractProblemThe aim of this study was to ascertain the associations between serum leptin levels and chronic epididymitis and semen parameters in patients with infertility.Method of studyA total of 846 patients who were diagnosed as primary infertility were enrolled and divided into four groups. The general information, blood lipids, reproductive hormones, and semen parameters were collected. Receiver operating characteristic (ROC) curves of leptin were plotted for diagnosis of the poor sperm quality. We used Student'sttest and the chi‐squared test to analyze their relationships and used logistic regression analysis to evaluate potential confounding factors.ResultsReceiver operating characteristic curve revealed that leptin had better sensitivity and specificity at the concentration of 6.02 (0.565 and 0.917). Isolated epididymitis or elevated leptin had no effect on sperm concentration and sperm membrane function, but the combination of these conditions would reduce the concentration and normal morphology rate both (P = 0.002,P = 0.005). Epididymitis or elevated leptin can affect the motility of sperms, the former presented more significance (P = 0.000), and the co‐existence would further reduce the sperm motility (P = 0.001).ConclusionLow sperm motility and sperm normal morphology were found to be associated with chronic epididymitis and high leptin. Simultaneously suffering from chronic epididymitis and high leptin could produce a more serious effect on sperm quality.