bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health and social care costs at the end of life: a matched analysis of linked patient records in East London
Authors: Jayatunga Wikum , Lewer Dan , Shand Jenny , Sheringham Jessica , Morris Stephen , George Julie 
Published: October 2019

Abstract

Background
care in the final year of life accounts for 10% of inpatient hospital costs in UK. However, there has been little analysis of costs in other care settings. We investigated the publicly funded costs associated with the end of life across different health and social care settings.

Method
we performed cross-sectional analysis of linked electronic health records of residents aged over 50 in a locality in East London, UK, between 2011 and 2017. Those who died during the study period were matched to survivors on age group, sex, deprivation, number of long-term conditions and time period. Mean costs were calculated by care setting, age and months to death.

Results
across 8,720 matched patients, the final year of life was associated with £7,450 (95% confidence interval £7,086–£7,842, P &lt; 0.001) of additional health and care costs, 57% of which related to unplanned hospital care. Whilst costs increased sharply over the final few months of life in emergency and inpatient hospital care, in non-acute settings costs were less concentrated in this period. Patients who died at older ages had higher social care costs and lower healthcare costs than younger patients in their final year of life.

Conclusions
the large proportion of costs relating to unplanned hospital care suggests that end-of-life planning could direct care towards more appropriate settings and lead to system efficiencies. Death at older ages results in an increasing proportion of care costs relating to social care than to healthcare, which has implications for an ageing society.
Life-Expectancy Disparities Among Adults With HIV in the United States and Canada: The Impact of a Reduction in Drug- and Alcohol-Related Deaths Using the Lives Saved Simulation Model
Authors: Althoff Keri N , Chandran Aruna , Zhang Jinbing , Arevalo Wendy Miranda , Gange Stephen J , Sterling Timothy R , Gill M John , Justice Amy C , Palella Frank J , Rebeiro Peter F , Silverberg Michael J , Mayor Angel M , Horberg Michael A , Thorne Jennifer E , Rabkin Charles S , Mathews W Christopher , Klein Marina B , Humes Elizabeth , Lee Jennifer , Hogg Robert , Moore Richard D ,   North American AIDS Cohort Collaboration on Research and Design (NA-ACCORD) of IeDEA
Published: October 2019

Abstract
Improvements in life expectancy among people living with human immunodeficiency virus (PLWH) receiving antiretroviral treatment in the United States and Canada might differ among key populations. Given the difference in substance use among key populations and the current opioid epidemic, drug- and alcohol-related deaths might be contributing to the disparities in life expectancy. We sought to estimate life expectancy at age 20 years in key populations (and their comparison groups) in 3 time periods (2004–2007, 2008–2011, and 2012–2015) and the potential increase in expected life expectancy with a simulated 20% reduction in drug- and alcohol-related deaths using the novel Lives Saved Simulation model. Among 92,289 PLWH, life expectancy increased in all key populations and comparison groups from 2004–2007 to 2012–2015. Disparities in survival of approximately a decade persisted among black versus white men who have sex with men and people with (vs. without) a history of injection drug use. A 20% reduction in drug- and alcohol-related mortality would have the greatest life-expectancy benefit for black men who have sex with men, white women, and people with a history of injection drug use. Our findings suggest that preventing drug- and alcohol-related deaths among PLWH could narrow disparities in life expectancy among some key populations, but other causes of death must be addressed to further narrow the disparities.
A Counter-Culture of Law: Jurisprudential Change and the Intellectual Origins of the Critical Legal Studies Movement
Authors: Salojärvi Juhana 
Published: October 2019

Abstract
Critical Legal Studies Movement (CLS) was a critical jurisprudential movement that developed during the 1970s, and came into being in 1977 when the first conference on critical legal studies was held. This article traces the intellectual origins of CLS, providing a comprehensive analysis on its history. The movement was, this article argues, a radical extension of the changes that had occurred in legal scholarship during the 1960s and early 1970s. Moreover, it was a result of a complex mixture of intellectual, scholarly, and political aspects, and it should not be reduced into any one of them.
Computerized stimuli for studying oddity effects
Authors: Dobbinson Khia E , Skarratt Paul A , Morrell Lesley J 
Published: October 2019

Abstract
Visually hunting predators must overcome the challenges that prey groups present. One such challenge is the confusion effect where an overburdened visual system means predators are unable to successfully target prey. A strategy to overcome confusion is the targeting of distinct, or odd, individuals (the oddity effect). In live prey experiments, manipulation of group member phenotypes can be challenging and prey may differ on more than the single feature one intends to define as odd. The use of highly controllable computerized stimuli to study predator–prey interactions is increasingly popular in the field of behavioral ecology. However, to our knowledge, the validity of computerized stimuli to study the oddity effect has not been established. Predator choice experiments were conducted using naive stickleback predators to ascertain whether the oddity effect could be demonstrated in the absence of live prey. We found evidence for both the oddity effect and preferential targeting of group edges and low-density regions, as would be predicted if predators targeted prey individuals to minimize confusion. The oddity effect was evident at a low threshold, above which dots were no longer perceived as odd and no longer attacked more often than expected by chance. We conclude that computerized stimuli are an improved, practical method for studying oddity effects while further validating the use of similar methods for studying other aspects of visual predation. In addition to higher control of “prey” appearance, the replacement of live prey animals with digital stimuli is ethically beneficial and reusing code improves experimental efficiency.
Order and disorder: abnormal 3D chromatin organization in human disease
Authors: Anania Chiara , Lupiáñez Darío G 
Published: October 2019

Abstract
A precise three-dimensional (3D) organization of chromatin is central to achieve the intricate transcriptional patterns that are required to form complex organisms. Growing evidence supports an important role of 3D chromatin architecture in development and delineates its alterations as prominent causes of disease. In this review, we discuss emerging concepts on the fundamental forces shaping genomes in space and on how their disruption can lead to pathogenic phenotypes. We describe the molecular mechanisms underlying a wide range of diseases, from the systemic effects of coding mutations on 3D architectural factors, to the more tissue-specific phenotypes resulting from genetic and epigenetic modifications at specific loci. Understanding the connection between the 3D organization of the genome and its underlying biological function will allow a better interpretation of human pathogenesis.
Feature selection may improve deep neural networks for the bioinformatics problems
Authors: Chen Zheng , Pang Meng , Zhao Zixin , Li Shuainan , Miao Rui , Zhang Yifan , Feng Xiaoyue , Feng Xin , Zhang Yexian , Duan Meiyu , Huang Lan , Zhou Fengfeng 
Published: October 2019

Abstract

Motivation
Deep neural network (DNN) algorithms were utilized in predicting various biomedical phenotypes recently, and demonstrated very good prediction performances without selecting features. This study proposed a hypothesis that the DNN models may be further improved by feature selection algorithms.

Results
A comprehensive comparative study was carried out by evaluating 11 feature selection algorithms on three conventional DNN algorithms, i.e. convolution neural network (CNN), deep belief network (DBN) and recurrent neural network (RNN), and three recent DNNs, i.e. MobilenetV2, ShufflenetV2 and Squeezenet. Five binary classification methylomic datasets were chosen to calculate the prediction performances of CNN/DBN/RNN models using feature selected by the 11 feature selection algorithms. Seventeen binary classification transcriptome and two multi-class transcriptome datasets were also utilized to evaluate how the hypothesis may generalize to different data types. The experimental data supported our hypothesis that feature selection algorithms may improve DNN models, and the DBN models using features selected by SVM-RFE usually achieved the best prediction accuracies on the five methylomic datasets.

Availability and implementation
All the algorithms were implemented and tested under the programming environment Python version 3.6.6.

Supplementary information
Supplementary data are available at Bioinformatics online.
Disruptive selection of shell colour in land snails: a mark–recapture study of Euhadra peliomphala simodae
Authors: Ito Shun , Konuma Junji 
Published: October 2019

AbstractMany theoretical studies have suggested that disruptive selection plays an important role in phenotypic divergence, but few studies have determined the action of disruptive selection on phenotypic divergence via field studies. This study investigated the effect of disruptive selection on shell colour polymorphism in the Japanese land snail Euhadra peliomphala simodae to determine whether extreme phenotypes of snail shell colour are favoured over intermediate phenotypes. We conducted field surveys on an oceanic island with black, yellow and intermediate-coloured E. p. simodae snails. We captured and marked ~1800 individual snails and monitored their survival over 18 months. We quantified shell colours against images and examined the frequency distribution of shell colour variation. The variation exhibited a bimodal distribution with a far lower frequency of intermediate-coloured snails than of black or yellow snails. The population sizes of the three snail groups fluctuated synchronously with the changing seasons. Bayesian estimates showed lower survival rates for juvenile intermediate-coloured snails than for juvenile black and yellow snails, implying there was disruptive selection associated with shell colour. We suggest this disruptive selection may have resulted in the evolutionary divergence of the snail’s shell colour within the lineage having high shell colour variation.
Chinese Practice of Foucault’s ‘Disciplinary Power’ and its Effects on the Rehabilitation of Female Prisoners in China
Authors: Zhang Ao 
Published: October 2019

AbstractThe prison system of the People’s Republic of China (PRC) has been viewed by some scholars as effective in achieving the primary target of Chinese imprisonment: rehabilitation. This article aims to redress this argument. Drawing on interviews undertaken with 30 female parolees/ex-prisoners and 10 prison officers, this article argues that the Jifen Kaohe System in prison, which is strongly imprinted with Foucault’s theory of ‘disciplinary power’, is ineffective and is unable to make accurate judgements on the achievement of prisoners’ rehabilitation at selected women’s prisons. This article suggests that Foucault’s disciplinary power is theoretically problematic in producing a ‘new person’, not the least of its unawareness of contextualized culture, values and situations, and women’s agency in prison.
Multiple myeloma BM-MSCs increase the tumorigenicity of MM cells via transfer of VLA4-enriched microvesicles
Authors: Dabbah Mahmoud , Jarchowsky-Dolberg Osnat , Attar-Schneider Oshrat , Tartakover Matalon Shelly , Pasmanik-Chor Metsada , Drucker Liat , Lishner Michael 
Published: October 2019

Abstract
Multiple myeloma (MM) cells accumulate in the bone marrow (BM) where their interactions impede disease therapy. We have shown that microvesicles (MVs) derived from BM mesenchymal stem cells (MSCs) of MM patients promote the malignant traits via modulation of translation initiation (TI), whereas MVs from normal donors (ND) do not. Here, we observed that this phenomenon is contingent on a MVs’ protein constituent, and determined correlations between the MVs from the tumor microenvironment, for example, MM BM-MSCs and patients’ clinical characteristics. BM-MSCs’ MVs (ND/MM) proteomes were assayed (mass spectrometry) and compared. Elevated integrin CD49d (X80) and CD29 (X2) was determined in MM-MSCs’ MVs and correlated with patients’ staging and treatment response (free light chain, BM plasma cells count, stage, response to treatment). BM-MSCs’ MVs uptake into MM cell lines was assayed (flow cytometry) with/without integrin inhibitors (RGD, natalizumab, and anti-CD29 monoclonal antibody) and recipient cells were analyzed for cell count, migration, MAPKs, TI, and drug response (doxorubicin, Velcade). Their inhibition, particularly together, attenuated the uptake of MM-MSCs MVs (but not ND-MSCs MVs) into MM cells and reduced MM cells’ signaling, phenotype, and increased drug response. This study exposed a critical novel role for CD49d/CD29 on MM-MSCs MVs and presented a discriminate method to inhibit cancer promoting action of MM-MSCs MVs while retaining the anticancer function of ND-MSCs-MVs. Moreover, these findings demonstrate yet again the intricacy of the microenvironment involvement in the malignant process and highlight new therapeutic avenues to be explored.
Separation of 3-Hydroxy-3-Methylglutaryl Flavonoid Glycosides from Oxytropis falcata Using 2D Preparative Chromatography
Authors: Chen Chen , Zhao Jingyang , Chen Jilin , Chi Ming , Liu Yuchao 
Published: October 2019

Abstract
Oxytropis falcata Bunge, known as “the King of Herbs” in Tibetan medicine, is used for treatment of hyperpyrexia, pain, wounds, inflammation and anthrax. However, it is difficult to isolate compound with high-purity from O. falcata because of its complexity. In this work, an efficient method was successfully established for the separation of 3-hydroxy-3-methylglutaryl (HMG) flavonoid glycosides from O. falcata by 2D preparative chromatography (2D-HPLC). An ODS C18 preparative column was used for the first-dimension preparation, and the XCharge C18 preparative column with different separation selectivity to the ODS C18 stationary phase was used for the second-dimension preparation. Four HMG flavonoid glycosides (oxytroflavosides A–D) and two flavonoid glycosides (oxytroflavosides F and G) were separated with purities over 98%. Their structures were elucidated by nuclear magnetic resonance spectroscopy. The 2D-HPLC method used in this study is effective for preparative separation of HMG flavonoid glycosides from O. falcata. Additionally, this method shows great potential for the separation of flavonoid glycosides from other plant extracts.
Molecularly Imprinted Polymer Nanoparticles for Selective Solid Phase Extraction of Fluvoxamine in Human Urine and Plasma
Authors: Soleimani Mojtaba , Daryasari Ameneh Porgham , Joshani Parisa 
Published: October 2019

Abstract
In this work, the molecularly imprinted polymer nanoparticles (MIP-NPs) for the selective determination of fluvoxamine have been described. The polymer nanoparticles were synthesized by the polymerization of methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, 2,2-azobisisobutyronitrile as an initiator and fluvoxamine as a template molecule. The MIP-NPs were characterized using techniques that included Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). Imprinted fluvoxamine molecules were removed from the polymeric structure using acetonitrile in methanol (2:8; v/v) as the eluting solvent. The linear dynamic range for fluvoxamine was 10–1200 μg L−1. The developed method was successfully applied to the extraction of fluvoxamine in complex biological samples.
Hypoxia alters vulnerability to capture and the potential for trait-based selection in a scaled-down trawl fishery
Authors: Thambithurai Davide , Crespel Amelie , Norin Tommy , Rácz Anita , Lindström Jan , Parsons Kevin J , Killen Shaun S 
Published: October 2019

Lay summarySelective harvest of wild organisms by humans can influence the evolution of plants and animals, and fishing is recognized as a particularly strong driver of this process. Importantly, these effects occur alongside environmental change. Here we show that aquatic hypoxia can alter which individuals within a fish population are vulnerable to capture by trawling, potentially altering the selection and evolutionary effects stemming from commercial fisheries.
A key role for the novel coronary artery disease gene JCAD in atherosclerosis via shear stress mechanotransduction
Authors: Douglas Gillian , Mehta Vedanta , Al Haj Zen Ayman , Akoumianakis Ioannis , Goel Anuj , Rashbrook Victoria S , Trelfa Lucy , Donovan Lucy , Drydale Edward , Chuaiphichai Surawee , Antoniades Charalambos , Watkins Hugh , Kyriakou Theodosios , Tzima Ellie , Channon Keith M 
Published: October 2019

Abstract

Aims
Genome-wide association studies (GWAS) have consistently identified an association between coronary artery disease (CAD) and a locus on chromosome 10 containing a single gene, JCAD (formerly KIAA1462). However, little is known about the mechanism by which JCAD could influence the development of atherosclerosis.

Methods and results
Vascular function was quantified in subjects with CAD by flow-mediated dilatation (FMD) and vasorelaxation responses in isolated blood vessel segments. The JCAD risk allele identified by GWAS was associated with reduced FMD and reduced endothelial-dependent relaxations. To study the impact of loss of Jcad on atherosclerosis, Jcad−/− mice were crossed to an ApoE−/− background and fed a high-fat diet from 6 to16 weeks of age. Loss of Jcad did not affect blood pressure or heart rate. However, Jcad−/−ApoE−/− mice developed significantly less atherosclerosis in the aortic root and the inner curvature of the aortic arch. En face analysis revealed a striking reduction in pro-inflammatory adhesion molecules at sites of disturbed flow on the endothelial cell layer of Jcad−/− mice. Loss of Jcad lead to a reduced recovery perfusion in response to hind limb ischaemia, a model of altered in vivo flow. Knock down of JCAD using siRNA in primary human aortic endothelial cells significantly reduced the response to acute onset of flow, as evidenced by reduced phosphorylation of NF-КB, eNOS, and Akt.

Conclusion
The novel CAD gene JCAD promotes atherosclerotic plaque formation via a role in the endothelial cell shear stress mechanotransduction pathway.
Resistant hypertension: new insights and therapeutic perspectives
Authors: Ruilope Luis M , Rodríguez-Sánchez Elena , Navarro-García José Alberto , Segura Julian , Órtiz Alberto , Lucia Alejandro , Ruiz-Hurtado Gema 
Published: October 2019

Abstract
Resistant hypertension (RH) is a concept that currently goes beyond the classical definition of blood pressure ≥140/90 mmHg in subjects receiving three or more drugs of different classes at maximally tolerated doses. Here, we review the clinical relevance of RH and the different types of RH-associated phenotypes, namely refractory hypertension, controlled resistant hypertension, and masked uncontrolled hypertension. We also discuss current drug strategies and future treatments for these high-risk phenotypes.
Full sternotomy and minimal access approaches for surgical aortic valve replacement: a multicentre propensity-matched study
Authors: Paparella Domenico , Malvindi Pietro Giorgio , Santarpino Giuseppe , Moscarelli Marco , Guida Piero , Fattouch Khalil , Margari Vito , Martinelli Luigi , Albertini Alberto , Speziale Giuseppe 
Published: October 2019

AbstractOBJECTIVESSurgical aortic valve replacement (AVR) can be performed via a full sternotomy or a minimal access approach (mini-AVR). Despite long-term experience with the procedure, mini-AVR is not routinely adopted. Our goal was to compare contemporary outcomes of mini-AVR and conventional AVR in a large multi-institutional national cohort.METHODSA total of 5801 patients from 10 different centres who had a mini-AVR (2851) or AVR (2950) from 2011 to 2017 were evaluated retrospectively. Standard aortic prostheses were used in all cases. The use of the minimally invasive approach has increased over the years. The primary outcome is the incidence of 30-day deaths following mini-AVR and AVR. Secondary outcomes are the occurrence of major complications following both procedures. Propensity-matched comparisons were performed based on the multivariable logistic regression model.RESULTSIn the overall population patients who had AVR had an increased surgical risk based on the EuroSCORE, and the 30-day mortality rate was higher (1.5% and 2.3% in mini-AVR and AVR, respectively; P = 0.048). Propensity scores identified 2257 patients per group with similar baseline profiles. In the matched groups, patients who had mini-AVR, despite longer cardiopulmonary bypass (81 ± 32 vs 76 ± 28 min; P = 0.004) and cross-clamp (64 ± 24 vs 59 ± 21 min; P ≤ 0.001) times, had lower 30-day mortality rates (1.2% vs 2.0%; P = 0.036), reduced low cardiac output (0.8% vs 1.4%; P = 0.046) and reduced postoperative length of stay (9 ± 8 vs 10 ± 7 days; P = 0.004). Blood transfusions (36.4% vs 30.8%; P ≤ 0.001) and atrial fibrillation (26.0% vs 21.5%, P ≤ 0.001) were higher in patients who had the mini-AVR.CONCLUSIONSIn a large multi-institutional recent cohort, minimal access approach aortic valve replacement is associated with reduced 30-day mortality rates and shorter postoperative lengths of stay compared to standard sternotomy. A prospective randomized trial is needed to overcome the possible biases of a retrospective study.
CEPI: Driving Progress Toward Epidemic Preparedness and Response
Authors: Gouglas Dimitrios , Christodoulou Mario , Plotkin Stanley A , Hatchett Richard 
Published: October 2019

Abstract
The Coalition for Epidemic Preparedness Innovations (CEPI) was formed in the aftermath of the 2014–2015 Ebola outbreak in west Africa to support the development of vaccines that could improve the world’s preparedness against outbreaks of epidemic infectious diseases. Since its launch in 2017, CEPI has mobilized more than US$750 million to support its mission to develop vaccines against agents such as Lassa virus, Middle East respiratory syndrome coronavirus, and Nipah virus, as well as several rapid-response vaccine platforms to accelerate response times to unexpected epidemic threats. CEPI has also played a leading role in fostering institutional partnerships between public- and private-sector organizations to optimize allocation of resources for vaccine development against its priority pathogens. CEPI’s priorities include diversification of its current vaccine research and development investment portfolio to include additional pathogens, such as Rift Valley fever and chikungunya; establishment of technical and regulatory pathways for vaccine development across CEPI’s portfolio; development of sustainable manufacturing solutions for vaccine candidates nearing completion of safety and immunogenicity testing in humans; and creation of investigational stockpiles of its vaccine candidates for use in emergency situations. This commentary provides an overview of the global health challenges CEPI was established to address and its achievements to date, and indicates priorities for funding and coordination in the coming years.