bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of Ibuprofen and Phenylephrine in Tablets by High-Performance Thin Layer Chromatography and in Plasma by High-Performance Liquid Chromatography with Diode Array Detection
Authors: Ragab Marwa A A , Abdel-Hay Mohamed H , Ahmed Hytham M , Mohyeldin Sara M 
Published: March 2019

Abstract
Two chromatographic methods (high performance thin layer chromatography (HPTLC) and high performance liquid chromatography–diode array detector (HPLC-DAD)), were addressed for the analysis of a mixture consisted of phenylephrine hydrochloride and ibuprofen in two forms bulk and their combined dosage form. This binary mixture is considered to be a challenging one as the two drugs differ greatly in their chemical and physical properties. Not only this affects their simultaneous analysis, but also hinders their simultaneous extraction from biological fluids as plasma. That is the reason the literature lacks any report for the simultaneous extraction and analysis of these drugs from biological fluids. The concentration ranges of both drugs were 0.1–2.5 μg/spot and 0.1–100 μg/mL by HPTLC and HPLC, respectively. Not only was the HPLC-DAD method applied to the investigated drugs determination in pharmaceutical preparations, but also in spiked human plasma. Extensive study was conducted to optimize their simultaneous extraction from plasma as it was a crucial step for the in vivo analysis. The results obtained by proposed methods and a reference one were statistically comparable by analysis of variance test. No significant difference was recorded between the mean percent levels determined by the proposed methods and the reference one.
Emerging zoonotic viral infections of occupational health importance
Authors: Vonesch Nicoletta , Binazzi Alessandra , Bonafede Michela , Melis Paola , Ruggieri Anna , Iavicoli Sergio , Tomao Paola 
Published: March 2019

ABSTRACTEmerging viral infections represent a public health risk pointed out by the spreading of pathogens with potential zoonotic risk. Moreover, the risk of zoonosis has probably been underestimated in occupational settings. A literature review between 2007 and 2018 was performed to identify evidences concerning the epidemiological associations between some emerging viruses and occupational diseases. Observational studies and case-reports were selected and analyzed. West Nile Virus (WNV) disease, Crimean-Congo Hemorrhagic Fever (CCHF) disease and Hepatitis E virus (HEV) infection were included in the review for their potential zoonotic transmission. The most important risk factor for acquiring WNV infection and CCHF infection is the exposure to infected mosquitoes and ticks, respectively; therefore, outdoor workers are at risk of infection. HEV is responsible for epidemics and endemics of acute hepatitis in humans, that can become infected through waterborne, foodborne and zoonotic transmission routes. A total of 10, 34 and 45 eligible studies for WNV, CCHF virus (CCFHV) and HEV, respectively, were analyzed by year, country, study design, risk group and outcomes. The occupational risk groups mainly included farm and agricultural workers, veterinarians, slaughterers, animal handlers, healthcare workers and soldiers. These findings support the need to develop effective interventions to prevent transmission of emerging viruses.
Intestinal dysbiosis promotes epithelial-mesenchymal transition by activating tumor-associated macrophages in ovarian cancer
Authors: Xu Shuyun , Liu Zhenzhen , Lv Meihua , Chen Yuli , Liu Ya 
Published: March 2019

ABSTRACT
We aimed to investigate the relationship of intestinal dysbiosis (IDB) and ovarian cancer progression, and understand its underlying signaling mechanisms. IDB was induced with high dose antibiotics. The xenograft mouse model was used to assess the tumor progression. Real-time polymerase chain reaction and immunoblotting are commonly used quantitative methods, and they were used to quantify epithelial-mesenchymal transition (EMT) markers in this paper. Meanwhile, cellular proliferation was also measured. First, IDB could promote the growth of xenograft tumors and induce the EMT. Serum levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6 also increased remarkably. In addition, the production and secretion of TNF-α and IL-6 in macrophages isolated from IDB model mice were observably higher. In vitro, conditioned medium could significantly stimulate the development of EMT in ovarian cancer cells. Loss of macrophages completely offset the pro-tumor effects of IDB. IDB can stimulate the activation of tumor-associated macrophages in ovarian cancer, which is achieved by secreting pro-inflammatory cytokines IL-6 and TNF-α, and ultimately induces the development of EMT.
Use of Chemical and Nonchemical Methods for the Control of Varroa destructor (Acari: Varroidae) and Associated Winter Colony Losses in U.S. Beekeeping Operations
Authors: Haber Ariela I , Steinhauer Nathalie A , vanEngelsdorp Dennis 
Published: March 2019

Abstract
The parasitic mite Varroa destructor (Acari: Varroidae) is a major cause of overwintering honey bee (Apis mellifera) colony losses in the United States, suggesting that beekeepers must control Varroa populations to maintain viable colonies. Beekeepers have access to several chemical varroacides and nonchemical practices to control Varroa populations. However, no studies have examined large-scale patterns in Varroa control methods in the United States. Here we used responses from 4 yr of annual surveys of beekeepers representing all regions and operation sizes across the United States to investigate use of Varroa control methods and winter colony losses associated with use of different methods. We focused on seven varroacide products (amitraz, coumaphos, fluvalinate, hop oil, oxalic acid, formic acid, and thymol) and six nonchemical practices (drone brood removal, small-cell comb, screened bottom boards, powdered sugar, mite-resistant bees, and splitting colonies) suggested to aid in Varroa control. We found that nearly all large-scale beekeepers used at least one varroacide, whereas small-scale beekeepers were more likely to use only nonchemical practices or not use any Varroa control. Use of varroacides was consistently associated with the lowest winter losses, with amitraz being associated with lower losses than any other varroacide product. Among nonchemical practices, splitting colonies was associated with the lowest winter losses, although losses associated with sole use of nonchemical practices were high overall. Our results suggest potential control methods that are effective or preferred by beekeepers and should therefore inform experiments that directly test the efficacy of different control methods. This will allow beekeepers to incorporate Varroa control methods into management plans that improve the overwintering success of their colonies.
The course of the Orphan Stream in the Northern Galactic hemisphere traced with Gaia DR2
Authors: Fardal Mark A , van der Marel Roeland P , Sohn Sangmo Tony , del Pino Molina Andres 
Published: March 2019

Abstract
The Orphan Stream is one of the most prominent tidal streams in the Galactic halo. Using data on red giants, RR Lyrae, and horizontal branch stars from Gaia and other surveys, we determine the proper motion of the Orphan Stream over a path of more than 90° on the sky. We also provide updated tracks for the sky position, distance, and radial velocity of the stream. Our tracks in these latter dimensions mostly agree with previous results. However, there are significant corrections to the earlier distance and latitude tracks as the stream approaches the Galactic disc. Stream stars selected with three-dimensional kinematics display a very tight red giant sequence. Concordantly, we find that applying a proper motion cut removes the most metal-rich stars from earlier spectroscopic samples of stream stars, though a significant dispersion remains, indicating a dwarf galaxy origin. The deceleration of the stream towards its leading end suggests a circular velocity of $\sim \!200 \, \mbox{km}\, \mbox{s}^{-1}$ at a galactocentric radius $\sim \!30 \, \mbox{kpc}$, consistent with other independent evidence. However, the track of the stream departs significantly from an orbit; the spatial track does not point along the same direction as the velocity vector, and it exhibits a lateral wiggle that is unlikely to match any reasonable orbit. The low metallicity and small dispersion of the stream in the various coordinates point to a progenitor with a relatively low dynamical mass $\sim \!10^8 \, \mathrm{ M}_{\odot }$.
Opacity, variability, and kinematics of AGN jets
Authors: Kutkin A M , Pashchenko I N , Sokolovsky K V , Kovalev Y Y , Aller M F , Aller H D 
Published: March 2019

ABSTRACT
Synchrotron self-absorption in active galactic nuclei (AGN) jets manifests itself as a time delay between flares observed at high and low radio frequencies. It is also responsible for the observing frequency-dependent change in size and position of the apparent base of the jet, aka the core shift effect, detected with very long baseline interferometry (VLBI). We measure the time delays and the core shifts in 11 radio-loud AGN to estimate the speed of their jets without relying on multi-epoch VLBI kinematics analysis. The 15–8 GHz total flux density time lags are obtained using Gaussian process regression, the core shift values are measured using VLBI observations and adopted from the literature. A strong correlation is found between the apparent core shift and the observed time delay. Our estimate of the jet speed is higher than the apparent speed of the fastest VLBI components by the median coefficient of 1.4. The coefficient ranges for individual sources from 0.5 to 20. We derive Doppler factors, Lorentz factors, and viewing angles of the jets, as well as the corresponding de-projected distance from the jet base to the core. The results support evidence for acceleration of the jets with bulk motion Lorentz factor Γ ∝ R0.52±0.03 on de-projected scales R of 0.5–500 parsecs.
Milky Way globular clusters in γ-rays: analysing the dynamical formation of millisecond pulsars
Authors: de Menezes Raniere , Cafardo Fabio , Nemmen Rodrigo 
Published: March 2019

ABSTRACT
Globular clusters (GCs) are evolved stellar systems containing entire populations of millisecond pulsars (MSPs), which are efficient γ-ray emitters. Observations of this emission can be used as a powerful tool to explore the dynamical processes leading to binary system formation in GCs. In this work, 9 yr of Fermi Large-Area Telescope data were used to investigate the γ-ray emission from all GCs in the Milky Way. Twenty-three clusters were found as γ-ray bright, with two of them never having been reported before. It was also found that magnetic braking probably has a smaller impact on the formation rate of binary systems in metal-rich GCs than previously suggested, while a large value for the two-body encounter rate seems to be a necessary condition. The influence of the encounter rate per formed binary was for the first time explored in conjunction with γ-ray data, giving evidence that if this quantity is very high, binary systems will get destroyed before having time to evolve into MSPs, thus decreasing the total number of MSPs in a GC. No extended emission was found even for clusters whose optical extent is ≈0.5°; all of them are point-like sources spatially in agreement with the optical cores of the GCs, supporting previous X-ray results of heavier objects sinking into the clusters’ cores via dynamical friction. The possibility of extrapolating these results to ultra-compact dwarf galaxies is discussed, as these systems are believed to be the intermediate case between GCs and dwarf galaxies.
Snake envenomation: is the 20 min whole blood clotting test (WBCT20) the optimum test for management?
Authors: Dsilva A A , Basheer A , Thomas K 
Published: March 2019

Abstract

Background
The 20 min whole blood clotting test (WBCT20) is a simple bedside test recommended by World Health Organization (WHO) to assess hemotoxic envenomation and guide administration of polyvalent anti-snake venom (ASV). However, reliability and validity of this test has not been well documented in literature.

Methods
Sixty consecutive patients with history of snake bite were prospectively evaluated at a teaching hospital in India over 2 years. Envenomation was established by clinical and laboratory criteria. WBCT20 was done at 0, 4 and 12 h using standardized protocol. Prothrombin time (PT) with international normalized ratio (INR) was estimated at similar intervals to detect venom-induced consumption coagulopathy. Sensitivity, specificity and likelihood ratios (LR) were determined for WBCT20 using envenomation criteria as gold standard. WBCT20 was compared with PT/INR at cutoff values of ≥1.4 and ≥1.2. Two observers performed test–retest correlation to determine inter-observer variability of WBCT20.

Results

Seventeen of 60 patients had evidence of hemotoxic envenomation. Four patients had combined neurotoxicity and hemotoxicity. Sensitivity and specificity of WBCT20 were 94 and 76%; positive and negative LR were 3.9 and 0.08, respectively. No inter-observer variability was noted.

Conclusions
WBCT20 is a highly sensitive test with excellent reliability for detecting envenomation. However, the false positive rate in this study was 24%. Asymptomatic snake bite patients with a positive WBCT20 but no corresponding clinical signs of envenomation should be tested using PT/INR before receiving ASV to prevent unnecessary waste of anti-venom.
The impact of journal re-grading on perception of ranking systems: Exploring the case of the Academic Journal Guide and Business and Management scholars in the UK
Authors: Walker James T , Salter Ammon , Fontinha Rita , Salandra Rossella 
Published: March 2019

AbstractThe marked increase in the use of metrics, such as journal lists, to assess research has had a profound effect on academics’ working lives. While some view the diffusion of rankings as beneficial, others consider their diffusion as a malicious development, which further acerbates a tendency towards managerialism in academia, and undermine the integrity and diversity of academic research. Using data from a large-scale survey and a re-grading of journals in a ranking used by Business and Management UK scholars—the Academic Journal Guide—as a pseudo-experiment, we examine what determines negative and positive perceptions of rankings. We find that the individuals who published in outlets that were upgraded were less hostile to the ranking than those who did not benefit from these changes, and that individuals were also less hostile to the ranking if outlets in their field had benefited from re-grading in the new list. We also find that the individuals who published in outlets that were upgraded were more positive to the ranking than those who did not benefit from these changes, and that individuals were also more positive to the ranking if outlets in their field had benefited from re-grading in the new list.
Increased expression of Fas on group 2 and 3 innate lymphoid cells is associated with an interferon signature in systemic lupus erythematosus and Sjögren’s syndrome
Authors: Blokland Sofie L M , van den Hoogen Lucas L , Leijten Emmerik F A , Hartgring Sarita A Y , Fritsch Ruth , Kruize Aike A , van Roon Joel A G , Radstake Timothy R D J 
Published: March 2019

Abstract

Objective
The role of innate lymphoid cells (ILCs) in the pathophysiology of rheumatic diseases is emerging. Evidence from animal studies implicate type I IFN, produced by plasmacytoid dendritic cells, to be involved in regulating the survival of group 2 and group 3 ILCs (ILC2s and ILC3s) via the upregulation of Fas (CD95) expression. For the first time, we explored the frequency and phenotype of circulating ILCs in SLE and primary Sjögren’s syndrome (pSS) in relationship to the IFN signature.

Methods
Frequencies and phenotypes of ILC subsets and plasmacytoid dendritic cells were assessed by flow cytometry in peripheral blood of patients with SLE (n = 20), pSS (n = 20) and healthy controls (n = 17). Patients were stratified by the presence or absence of an IFN signature as assessed by RT-qPCR on circulating mononuclear cells.

Results
ILC1 frequencies were increased in peripheral blood of patients with SLE as compared with healthy controls and correlate with disease activity in pSS patients. Overall, the frequencies of ILC2s or ILC3s did not differ between patients with SLE, pSS and healthy controls. However, patients with a high type I IFN signature expressed elevated levels of Fas on ILC2s and ILC3s, which coincided with decreased frequencies of these cells in blood.

Conclusion
The presence of a type I IFN signature is related to Fas expression and frequencies of circulating ILC2s and ILC3s in patients with SLE and pSS, potentially altering the homeostatic balance of ILCs.
LOCAL DRLS FOR PAEDIATRIC CT EXAMINATIONS BASED ON SIZE-SPECIFIC DOSE ESTIMATES IN KERMANSHAH, IRAN
Authors: Mohammadbeigi Ahmad , Khoshgard Karim , Haghparast Abbas , Eivazi Mohammad Taghi 
Published: March 2019

AbstractDue to the radiosensitivity of paediatric patients to X-ray, it is necessary to survey the paediatric DRLs using size-specific dose estimates (SSDE). In the present study, we determined the local diagnostic reference levels (DRLs) for paediatric chest, head and abdomen-pelvis CT examinations and their Surview scans in Kermanshah city, Iran. For ≤1 year, 1–5 years, 5–10 years and 10–15 years the DRLs (mGy) based on SSDE were determined N/A, 6.00, 6.25, 8.27 for abdomen-pelvis, and 8.74, 7.45, 11.15, 10.45 for chest and 19.05, 18.33, 18.22, 20.14 for head examinations, respectively. The differences between body size and default phantom defined in CT scanners are significant and should be considered when determining the DRLs. Based on our findings, use of CTDIv and SSDE parameters for determining DRLs leads to significant different results in children; thus SSDE is suggested as a more accurate index than CTDIV for establishing DRLs in paediatric CT examinations.
Support for My Video is Support for Me:
Authors: Forgeron Paula A. , McKenzie Emily , O’Reilly Jenna , Rudnicki Erika , Caes Line 
Published: March 2019

Objectives:
Adolescents are heavy users of social media as a venue to share experience and obtain information. Adolescents with chronic pain may be no different. Given that adolescents with chronic pain report feelings of social isolation, of being different, and lack peer understanding, social media may help them obtain social support. We conducted a scoping review of YouTube to identify how adolescents with chronic pain use this platform to connect with other adolescents.

Materials and Methods:
The terms “youth with chronic pain” and “teens with chronic pain” were entered into the YouTube search bar to locate videos. Videos in English, targeted at and including an adolescent with chronic pain were included. Videos were screened for eligibility until 20 consecutive videos listed on the main page were excluded. For each included video the first 5 related videos suggested by YouTube in the sidebar were also screened for eligibility.

Results:
This selection process resulted in 18 included videos, with a total of 936 viewer comments. Recurring comment themes were identified using qualitative content analysis. Video content mainly covered multidisciplinary treatment options, alternative treatments, and impact of pain on daily life. Although a variety of treatment options were discussed, details of treatment were lacking. Comments reflected the overarching message “you are not alone!” and mainly focused on providing and receiving support, sharing suffering, and revealing the impact of pain on relationships and daily life.

Discussion:
Despite potential challenges associated with social media, YouTube may be a promising platform for provision of social support for adolescents with chronic pain.
Educating Obstetrics and Gynecology Residents on Transgender Patients
Authors: Vinekar Kavita , Rush Shannon K. , Chiang Seine , Schiff Melissa A. 
Published: March 2019

OBJECTIVE:
To describe education on transgender health provided by obstetrics and gynecology residency programs and to identify the facilitators and barriers to providing this training.

METHODS:
We conducted a cross-sectional survey to evaluate transgender health education in residency among a representative sample of 100 of the 236 obstetrics and gynecology residency programs listed in the 2015 Electronic Residency Application Service catalogue. We compared programs that did and did not offer transgender education on demographics, presence and type of transgender education offered, and reasons for and barriers to offering transgender health education using χ2 or Fisher's exact testing.

RESULTS:
We found that 31 out of 61 (51%, 95% CI 38–64%) programs completing the survey offered transgender health education in residency. Compared with programs that offered no education, residency programs offering education were more likely to report that transgender health education was very important (19/31, 61% [95% CI 42–78%] vs 7/30, 23% [95% CI 9–42%]), resident interest (20/31, 64% [95% CI 45–81%] vs 11/30, 37% [95% CI 20–56%]), and the presence of a transgender population requesting services (28/31 or 90% [95% CI 74–98%] vs 16/30 or 53% [95% CI 34–72%]). Among the 31 programs that offered transgender health education, 30 (97%; 95% CI 83–99%) provided formal didactic sessions and 20 (64%; 95% CI 45–81%) offered health screening for both male-to-female and female-to-male transgender patients, but 17 (55%; 95% CI 36–73%) did not offer gender-affirming hormone therapy. Among the 30 programs that did not currently offer transgender health education, 24 (80%; 95% CI 61–92%) planned to establish a transgender education program in the next year.

CONCLUSION:
Our survey of obstetrics and gynecology residency programs highlights the interest in transgender health education for a systemically underserved population of patients.