bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous Determination of Four Monoamine Neurotransmitters and Seven Effective Components of Zaoren Anshen Prescription in Rat Tissue using UPLC-Ms/Ms
Authors: Li Rong , Wang Lin , Wang Xiao , Zhang Dian , Zhang Yafeng , Li Zhuo , Fang Minfeng 
Published: July 2019

Background:
Zaoren Anshen Prescription (ZAP) is widely used as a classic Chinese Traditional
Medicine (TCM) prescription for the treatment of palpitations and insomnia in China. Some
studies have identified the main active components for its anti-insomnia effect and observed changes
of some endogenous components that are closely related to its anti-insomnia effect. However, simultaneous
determination of four monoamine neurotransmitters and seven effective components of ZAP and
the investigation of their distribution in tissues by using ultra-performance liquid chromatography
with tandem mass spectrometry (UPLC-MS/MS) have not been reported.

Methods:
An ultra-performance liquid chromatography with tandem mass spectrometry method was
developed and validated for simultaneous quantification of four monoamine neurotransmitters (norepinephrine,
dopamine, 5-hydroxy tryptamine and 5-hydroxyindoleacetic acid) and seven prescription
components (danshensu, protocatechualdehyde, spinosin, 6´´´ -feruylspinosin, salviaolic acid B, schisandrin
and deoxyschisandrin) in rats’ tissues. Tissue samples were prepared by protein precipitation
with acetonitrile. Chromatographic separation was carried out on a C18 column with a gradient mobile
phase consisting of acetonitrile and 0.01% formic acid water. An electrospray ionization triple quadrupole
concatenation mass spectrometer was set to switch between positive and negative modes in single
run time. All the components were quantitated by multiple-reaction monitoring scanning.

Results:
: The lower limits of quantitation for all analytical components were 0.78 ng/mL-1.99 ng/mL in
the heart, liver, spleen, lung, kidney and brain. All the calibration curves displayed good linearity (r >
0.99544). The precision was evaluated by intra-day and inter-day assays, and the relative standard
deviation (RSD) values were all within 12.67%. The relative errors of the accuracy were all within ±
19.88%. The recovery ranged from 76.00% to 98.78% and the matrix effects of eleven components
were found to be between 85.10% and 96.40%.

Conclusion:
This method was successfully applied to study the distribution of seven components from
ZAP and the concentration changes of four monoamine neurotransmitters after oral ZAP in six tissues.
Electrolytic colouring of anodized aluminium on tin basis
Authors: Šopová Barbora , Zemanová Matilda 
Published: July 2019

Abstract
The aim of the study was to find parameters of electrolytic colouring on tin basis to form uniform black coatings on anodized aluminium. A two steps electrolytic process consisting of aluminium anodization in a sulphuric acid electrolyte and colouring in tin acidic electrolyte was used. Among parameters influencing the colouring process, AC colouring voltage, composition of the counter electrode and agitation of the colouring electrolyte were studied. Spectrocolorimetry was applied to analyse the quality of the colouring. Thickness of the coloured and sealed anodized specimens was also evaluated. Optimal parameters for uniform colouring of anodized specimens were found. Unfortunately, colouring electrolyte on tin basis is susceptible to oxidation which negatively influences the colouring.
Influence of hydrotalcite preparation conditions on its physico-chemical properties
Authors: Mališová Miroslava , Horňáček Michal , Hudec Pavol , Mikulec Jozef , Jorík Vladimír , Hájeková Elena 
Published: July 2019

Abstract
The aim of the research was to prepare and characterize hydrotalcite synthesized under different preparation conditions. The most common hydrotalcite preparation is the co-precipiaton method. The preparation process strongly influences the catalytic properties of hydrotalcite; therefore, optimal conditions have to be determined. During the study, seven samples of the catalyst were prepared in the pH range from 8 to 12 and the synthesis temperature range from 25 to 55 °C. Based on several catalyst properties, optimal synthesis pH 10 was found. Ideal temperature of the preparation was determined to be 35 °C, but the temperature does not have a significant effect on the catalyst properties.
Use of zeolites for macronutrients removal from wastewater
Authors: Urminská Barbora , Derco Ján , Zakhar Ronald , Korpicsová Adriana 
Published: July 2019

Abstract
Natural or synthetic zeolites have unique physical, chemical and structural properties that predetermine their use in many processes, including wastewater treatment. This study presents the results of our preliminary research in the field of nitrogen and phosphorus removal using adsorption and adsorptive ozonation with natural and modified zeolites. Iron-modified zeolite was the most efficient for the removal of ammonium nitrogen by adsorption. Phosphorus removal efficiency using adsorption was relatively low and natural zeolite was not suitable for the phosphorus removal at all. Ozone had no significant impact on the removal efficiency. Regeneration of loaded zeolites with ozone has also been studied. This method was partly efficient but it needs be further examined.
Budget transparency innovation in the Czech local government
Authors: Sedmihradská Lucie 
Published: July 2019

Abstract
Budget transparency innovations bring new extent and forms of transparency. The aim of the article is to explore the diffusion of budget explorers, that is, a budget transparency innovation extremely popular in the Czech Republic, and to evaluate their impact on voluntary budget information disclosed.
Careful mapping of the diffusion using a survey of budget explores in 72 former Czech district towns and media analysis shows that the key success factor was its convenience for politicians, as it is attractive, easy to implement and up-to-date demonstration of their transparency. Budget explorers are nowadays a standard extension to accounting software, and their usage is evaluated in several government transparency competitions.
The major benefit of the budget explorers is that they made for the first time publicly available detailed public financial information, changed the standard of best practice and drew some public attention. At the same time, they, unfortunately, narrowed the scope of the budget transparency debate by omitting the importance of the draft budget and introduction of performance measurement.
Local Political Business Cycles and Administration Spending in Polish Local Governments
Authors: Olejnik Łukasz Wiktor 
Published: July 2019

Summary

Subject and purpose of work: The purpose of this article is to verify the hypothesis of the occurrence of political business cycles at the self-governmental level in Poland.

Materials and methods: The article presents a quantitative analysis of expenditures on remuneration in the administration of Polish self-governments at the county (district) level (in the period 2007–2018) and at province (regional) level (in the period 1999–2018) with the use of Arellano-Bond and LSDVC estimators.

Results: The panel data analysis makes it possible to conclude that in Polish self-governments cyclical fluctuations of expenditure on remuneration can be observed. The increase in salaries depends on the power of the ruling coalition as well as unemployment and the output gap in a region. In the case of provinces and counties, also the increase of investment expenditures significantly affects the increase of salaries.

Conclusions: The results of the estimation of models clearly point to the presence of cyclical distortions in remuneration in public administration, which are caused by the upcoming elections.
Nectar and pollen production in ornamental cultivars of Prunus serrulata (Rosaceae)
Authors: Strzałkowska-Abramek Monika 
Published: July 2019

Abstract
Planting ornamental species can help to create pollinator-friendly habitats. In the present study the flowering phenology and floral reward (nectar and pollen) in two cultivars of Prunus serrulata (‘Kanzan’ and ‘Amanogawa’) were evaluated in 2014-2015 in the city of Lublin (south-east Poland). On average, the onset of flowering of P. serrulata occurred in the first ten days of April (‘Kanzan’) or at the end of April (‘Amanogawa’), and lasted 8-17 days. In each year of the study, P. serrulata ‘Kanzan’ started flowering 11-18 days earlier than P. serrulata ‘Amanogawa’. The double flowers of P. serrulata ‘Kanzan’ offered only tiny amounts of nectar and pollen. The total mass of sugars was 0.59 mg per flower and that of pollen 0.08 mg per flower. The semi-double flowers of P. serrulata ‘Amanogawa’ produced 4.5 mg of sugars per flower and 0.17 mg of pollen per flower. The frequency of pollinator visits to flowers was low in P. serrulata ‘Amanogawa’ and very low in P. serrulata ‘Kanzan’. Therefore, if pollinator-friendly arrangements are made in urban areas, these cultivars should not be planted.
Changes in the synthetic index of sustainable forest management at the level of regional directorates of the State Forests in 1993–2013
Authors: Kożuch Anna , Banaś Jan , Zięba Stanisław , Bujoczek Leszek 
Published: July 2019

AbstractThe aim of our work was to assess the direction of change taking place in the forests of the regional directorates of State Forests (rdSF) based on measurable indicators used to assess sustainable development. Based on a synthetic index (Z), changes in the years 1993−2013 were evaluated for individual directorates. We identified the regions with the highest and lowest rates of change in terms of sustainable development dynamics. The analysis was performed using spatiotemporal variables and the main criterion for selecting the diagnostic variables was their availability and comparability over the analysed period. The rdSF variation was assessed with the synthetic index (Z), using the method of zero unitarisation. In 1993−2013, favourable changes over time were indeed recorded, reflecting the progress in implementing practices supporting sustainable development in forestry. However, large differences exist between the regional managements in this respect. For the analysed period, the most favourable conditions from the perspective of sustainable forest management were maintained in rdSF Kraków, rdSF Białystok and rdSF Toruń, while the least desirable conditions were found in rdSF Zielona Góra, rdSF Piła and rdSF Warsaw. The greatest rates of beneficial change, on the other hand, were found in rdSF Szczecin, rdSF Kraków and rdSF Wrocław. In turn, the lowest rates of change of the synthetic index (Z) were observed in the directorates of Katowice, Piła and Łódź. In summary, measurable indicators of sustainable development are a good instrument for measuring the pace of change in sustainable forestry. They are an effective tool for assessing and reporting progress over time and should also be used when planning and implementing development strategies.
Spirocercosis in Dogs in Israel
Authors: Geva N. , Štrkolcová G. 
Published: July 2019

Abstract

Spirocerca lupi is a nematode causing spirocercosis disease that affects mostly carnivores and especially canidae. The life cycle of S. lupi includes a coprophagous beetle as an obligatory intermediate host and a variety of facultative paratenic hosts. In Israel, spirocercosis is considered to be a serious condition with a variety of clinical signs comprising a great risk to canine populations. The diagnosis at an early infective stage is unreliable and the vast majority of infected dogs are diagnosed only when the disease has advanced. In advanced stages of the disease, treatment is difficult and there is a high risk for complications. A study was carried out to compare the prevalence of S. lupi in the central region of Israel with a previous investigation and by that consequently try to estimate the efficacy of preventative treatment used nowadays in Israel. The study was done by the use of two different methods: looking for the infective larvae (L3) in the main intermediate host in Israel, Ontophagus sellatus, and searching for the eggs of S. lupi by performing flotation methods on faecal samples. Beetles and faecal samples were collected from four different locations in the winter and summer of 2017, 2018, and 2019. According to the literature review and collection of data from case studies, the prevalence of spirocercosis is increasing in Israel, despite the negative results from the dissections of beetles and faecal samples.
Assessment of Potential Clastogenic Effect of the Insecticide Mospilan® 20SP in Human Peripheral Blood Lymphocytes After in Vitro Exposure
Authors: Ničová K. , Schwarzbacherová V. , Galdíková M. , Holečková B. 
Published: July 2019

Abstract
Acetamiprid, that is known as the commercial formulation Mospilan® 20SP is the part of the neonicotinoid insecticide group and is widely used against various pests. In our study we assessed the potential clastogenic effects of Mospilan® in human peripheral blood lymphocytes in vitro using a chromosome aberration test. The lymphocytes were treated with acetamiprid in the concentration range of 5, 10, 25 and 50 µg.ml−1 for 24 and 48 h. After 24 h exposure, the insecticide induced statistically significant higher levels of chromosome aberrations from the concentration of 10 µg.ml−1 (P < 0.05 and P < 0.001) and a significant decrease in mitotic index (MI) at the concentrations of 25 and 50 µg.ml−1 (P < 0.05 and P < 0.01), respectively. After a 48 h exposure, we found a dose dependent increase in the percentage of chromosome aberrations at all concentrations (P < 0.05; P < 0.01 and P < 0.001) and a decrease in MI at concentrations of 25 and 50 µg.ml−1 (P < 0.05 and P < 0.01). Our results indicated that neonicotinoid insecticide formulations containing acetamiprid may have potential cytotoxic and genotoxic effects.
Analysis of Chromosomal Damage Caused by Acetamiprid
Authors: Stupáková K. , Galdíková M. , Schwarzbacherová V. , Holečková B. 
Published: July 2019

Abstract
Different chemicals can have genotoxic effects on the body, as confirmed by chromosome damage detection. Using conventional cytogenetic analysis and fluorescence in situ hybridization, we tested the extent of chromosome damage caused by the acetamiprid-based insecticide Mospilan 20SP on bovine peripheral blood lymphocytes at concentrations of, 2.5, 5, 25 and 50 µg.ml−1 after a 24 h incubation period. During the experiment, the presence of unstable aberrations—chromosomal and chromatid breaks and gaps—were detected by conventional cyto-genetic analysis. With increasing insecticide concentrations, we observed a statistically significant increase in chromosome damage frequency after 24 hours of exposure. Fluorescence in situ hybridization was used to detect stable structural aberrations; whole-chromosome painting probes for bovine chromosomes 1 and 7 (BTA 1 and BTA 7) were used for this purpose. As a result of exposure to the insecticide, neither BTA 1/BTA 7 translocations nor other types of translocations were observed.
Edible Spruce (Morchella esculenta), Accumulator of Toxic Elements in the Environment
Authors: Strapáč I. , Bedlovičová Z. , Baranová M. 
Published: July 2019

Abstract
In this study we examined the dried fruiting bodies of Morchella esculenta, collected in the area of the coal and biomass based thermal power plant in Vojany from the nearby Bahoň marsh, in the Slovak Republic. The area is characterized by a high environmental burden, especially because of air pollutant emissions from the power plant operation. Twenty-three (23) chemical elements were found in the dried fruiting bodies after microwave-assisted sample preparation using an Inductively Coupled Plasma Mass Spectrophotometer ICP-MS AGILENT 7500c system. The mercury content was determined employing a special AMA 254 apparatus intended for the determination of Hg directly in dry powdered fruiting bodies without microwave digestion. The content of toxic elements expressed in mg.kg−1 DW (dry weight) were as follows: Hg 0.048—0.052 (RSD—Relative Standard Deviation = 4.80 %); Cd 4.543—6.169 (RSD = 3.35 %); Pb 0.261—0.291 (RSD = 2.67 %); As 0.455—0.469 (RSD = 5.79 %); Cr 1.585—1.616 (RSD = 2.33 %); and Ni 8.166—9.276 (RSD = 3.03 %). The contents of cadmium, nickel and mercury exceeded the hygiene limits, while the contents of arsenic and lead approached the hygiene limits. Due to the high levels of toxic elements, the fruiting bodies collected in the location are not suitable for culinary purposes. The mushroom Morchella esculenta acts as an accumulator of toxic elements from the environment in which it grows and can be considered as an indicator of environmental pollution.
The role of folate receptor and reduced folate carrier polymorphisms in osteoporosis development
Authors: Mrozikiewicz Aleksandra E. , Bogacz Anna , Barlik Magdalena , Górska Aleksandra , Wolek Marlena , Kalak Małgorzata 
Published: July 2019

Summary

Introduction: Osteoporosis is a chronic metabolic disease with multifactorial etiology. One of possible osteoporosis causes may be impairment of osteoclasts function which leads to increased bone resorption. This may be a result of many metabolic changes. It is believed that changes of folate-methionine metabolism in osteoporosis play an essential role in the etiology of this disease.

Objective: The aim of this study was to examine how polymorphisms of SLC19A1 and FOLR3 genes may play the key role in folate-methionine pathway and influence on the etiology of osteoporosis.

Results: The statistically overrepresentation of mutated GG genotype of FOLR3 (rs11235449) was observed in the control group compared to the osteopenia (34.9% in osteopenia vs. 37.8% in controls, p=0.025, OR=0.61). As to the SLC19A1 (rs3788200) polymorphism we have noted the statistically significant over-representation of wild-type GG genotype (35.8% vs. 26.2%, p=0.046, OR=1.57) and overrepresentation of wild-type G allele (56.9% vs. 50.2%, p=0.061, OR=1.31) in osteopenia group if compared to the controls.

Conclusions: In our study we shown the protective role of mutated GG genotype of FOLR3 (rs11235449) polymorphism to osteopenia progress and possible role of wild-type GG genotype and wild-type G allele of SLC19A1 (rs3788200) polymorphism in osteopenia development.
Contribution of Polish agrotechnical studies on Cannabis sativa L. to the global industrial hemp cultivation and processing economy
Authors: Poniatowska Joanna , Wielgus Karolina , Szalata Milena , Szalata Marlena , Ożarowski Marcin , Panasiewicz Katarzyna 
Published: July 2019

Summary
Industrial hemp (Cannabis sativa L.) attracts increasing interest of growers, in relation to both industrial and medical purposes. Construction is the most promising area of the economy for hemp, and specifically, the fast-growing production of insulation and bio-composite materials.
The most significant factors affecting the final yield of hemp seeds and biomass include: climatic factors, i.e. influence of weather conditions that determines the content of cannabinoids in plants; genetic predisposition of the variety used as well as agrotechnical factors. The article provides information on the botanical characteristics of fiber hemp, hemp cultivation area, Polish monoecious varieties of industrial hemp, hemp's agricultural requirements, including: the demand for macroelements (nitrogen, phosphorus, potassium, calcium); choice of soil, pioneer crop, and field preparation; sowing timing and density; harvest time; water conditions; heat and light requirements for hemp. The review article is focused mainly on the results of studies carried out at the Institute of Natural Fibres and Medicinal Plants, data of the Agency for Restructuring and Modernization of Agriculture (ARiMR), Research Centre for Cultivar Testing (COBORU), European Industrial Hemp Association (EIHA), published in 2008 - 2018.
Changes in Triathletes’ Performance and Body Composition During a Specific Training Period for a Half-Ironman Race
Authors: Sellés-Pérez Sergio , Fernández-Sáez José , Férriz-Valero Alberto , Esteve-Lanao Jonathan , Cejuela Roberto 
Published: July 2019

Abstract
The number of recreational athletes completing a Half-Ironman triathlon has increased exponentially in recent years. However, there is a lack of research on how to train for this kind of an event. The purpose of this study was thus to analyse triathletes’ changes in performance and body composition following a triathlon-specific training period. Fourteen male amateur triathletes completed a 7-week period of general training and a 13-week period of specific training for a Half-Ironman triathlon. Anthropometric measures and performance tests were carried out to assess the effects of the specific training program. Results showed that the pre-test value of VO2max for cycling was inversely correlated not only with the percentage of change in cycling performance, but also with the percentage change in several variables of running performance. In swimming, inverse correlations were observed between the time of the first 800 m test and the time percentage change for this test, but not with the percentage change in the performance of other segments of the race. Moreover, the somatotype component of endomorphy and the fat mass percentage of the first anthropometry were highly correlated with the percentage change in VO2max in the run segment. These results highlight the importance of providing individualised training, considering that the same training program had a different impact on recreational triathletes belonging to the same group. Amateur athletes with higher initial performance levels probably need a greater amount of training to achieve improved adaptation.
Physical Performance During Soccer-7 Competition and Small-Sided Games in U12 Players
Authors: Sanchez-Sanchez Javier , Sanchez Mario , Hernández Daniel , Gonzalo-Skok Oliver , Casamichana David , Ramirez-Campillo Rodrigo , Nakamura Fabio Y. 
Published: July 2019

Abstract
The aim of this study was to compare the activity profile (external loads) during soccer-7 competition versus 6 vs 6 small-sided games ( SSGs) in U12 players. Peak velocity (Vmax), total distance completed (DT), total distance relative to match duration, the percentage of DT in acceleration (%DAC) and in deceleration (%DEA), and the percentage of DT at different speeds were recorded. Six types of SSGs were randomly implemented: without pitch orientation-delimitation and with a limit of three ball-contacts per player (3TOU), with no limit of ball-contacts (MAN), with a greater number of players as internal-offensive wildcard players (2WI) or external-offensive wildcard players (4WE); and with pitch orientation-delimitation and crossing the rival goal-line while dribbling the ball without goalkeepers (INV) or using official goalkeepers (GKP). The physical demands of SSGs were compared with the average of two soccer-7 match plays. During soccer-7 match plays a lower %DAC and %DEA (p < 0.05) were observed compared to 2WI, 4WE, INV and GKP, and to INV and GKP, respectively. The Vmax and %HI were greater (p < 0.05) in soccer-7 match plays compared to all SSGs. In conclusion, the demands imposed on U12 players during different formats of SSGs differ from the soccer-7 match play demands, presenting a low stimulation of the actions performed at high-speed and an adequate simulation of acceleration-deceleration actions.