bluuebunny/crossref_metadata_2025_split huggingface dataset:
Voltammetric Electronic Tongue Based on Carbon Paste Electrodes Modified with Biochar for Phenolic Compounds Stripping Detection
Authors: Kalinke Cristiane , de Oliveira Paulo Roberto , Bonet San Emeterio Marta , González‐Calabuig Andreu , del Valle Manel , Salvio Mangrich Antonio , Humberto Marcolino Junior Luiz , Bergamini Márcio F. 
Published: July 2019

AbstractBiochar is a charcoal produced from the biomass pyrolysis process that presents a highly porous and functionalized surface. In the present work an array of carbon paste electrodes (CPE) made of different forms of carbon (graphite, carbon nanotubes and activated biochar) was evaluated in the development of an electronic tongue for discrimination and stripping voltammetric determination of catechol (CAT), 4‐ethylcatechol (4‐EC) and 4‐ethylguaiacol (4‐EG) phenolic compounds. Morphological characterization of carbon materials and electrodes surfaces was performed by scanning electron microscopy (SEM) and semi‐quantitative elemental composition by energy dispersive spectroscopy (EDS). Electrochemical Impedance Spectroscopy (EIS) measurements were used for electrochemical characterization of electrodes. Cyclic voltammetry measurements were performed for the phenolic compounds evaluated using different concentrations. Principal component analysis (PCA) was performed to evaluate the qualitative analysis. Quantitative data modeling was done using artificial neural networks (ANN). The proposed sensor array presented analytical potentiality allowing the distinction and determination of CAT, 4‐EC and 4‐EG by using chemometric processing. The method showed sensibility, reproducibility and a good linearity (R2>0.9940) for three compounds evaluated. Spontaneous preconcentration of three compounds was possible using all three sensors, which can allow the application of these as passive samplers for remote determinations of phenolic compounds in wine and food samples.
Batch Injection Analysis with Amperometric Detection for DNA Biosensing Applications
Authors: Svitková Veronika , Labuda Ján , Vyskočil Vlastimil 
Published: July 2019

AbstractIn this work, batch injection analysis with the amperometric detection (BIA‐AD), employing a detection cell designed to adapt a screen‐printed carbon electrode (SPCE) was used for the first time as a robust electroanalytical system for DNA biosensing applications. The sensitive amperometric detection was used to evaluate the structural changes in double‐stranded DNA (dsDNA) after UV‐C irradiation of its solution for a given time. Batching of DNA samples was performed by precise electronic pipette microinjection of an irradiated sample aliquot onto the unmodified activated SPCE surface incorporated in the BIA‐AD system. Using the optimized experimental conditions (40 μL of 1 mg mL−1 dsDNA in a 0.1 M phosphate buffer of pH 7.4 sampled at the injection speed degree of 6 and detected at the potential of +1.5 V vs silver pseudo‐reference electrode), a time‐dependent response (gradual decrease of amperometric signal up to 58 % after 10 min of the irradiation) was found for the detection of damage to low molecular weight salmon sperm dsDNA. The advantages of this low‐dimensional and cost‐effective measuring system can be utilized not only for the quantification of DNA damage/degradation by UV irradiation, but they are also promising for studying other types of DNA interactions.
Electroanalysis of the Anthelmintic Drug Bithionol at Edge Plane Pyrolytic Graphite Electrode
Authors: Brycht Mariola , Konecka Kinga , Sipa Karolina , Skrzypek Sławomira , Mirčeski Valentin 
Published: July 2019

AbstractAn electrochemical study of the anthelmintic drug bithionol using edge plane pyrolytic graphite electrode (EPPGE) is presented for the first time by applying different electrochemical techniques, such as cyclic voltammetry (CV), square‐wave voltammetry (SWV), square‐wave adsorptive stripping voltammetry (SWAdSV), and alternating current (AC) impedance spectroscopy. Mechanistic aspects of the electrode reaction were studied implying a quasireversible electrode reaction from an adsorbed state of the reactant, coupled with a follow‐up chemical reaction to a final electroinactive product. The overall mechanism appears totally irreversible under conditions of CV at moderate scan rate, while being quasireversible under conditions of the fast SWV. Furthermore, an optimisation of the analytical procedure for quantitative determination of bithionol was conducted by applying SWV in an adsorptive stripping mode. The calibration curve was constructed in the concentration range of 0.1–1.0 μmol L−1 (R2=0.9984) with a sensitivity of 3.6 μA L μmol−1 and LOD of 26.7 nmol L−1. The simple and sensitive SWAdSV procedure was proved to be suitable for the analysis of spiked urine samples.
Coupling Electrochemical Adsorption and Long‐range Electron Transfer: Label‐free DNA Mismatch Detection with Ultramicroelectrode (UME)
Authors: Basu Nivedita , Ho Thu Huong , Guillon François‐Xavier , Zhang Yuanyuan , Bigey Pascal , Navakanta Bhat , Bedioui Fethi , Lazerges Mathieu 
Published: July 2019

AbstractA new electrochemical hybridization transduction pathway, obtained by coupling electrochemical adsorption and long‐range electron transfer through double‐stranded DNA, was investigated using ultramicroelectrode (UME). The results show that long‐range electron transfer does not occurs exclusively throws well‐packed and organized self‐assembled DNA monolayers. This approach is used to investigate long‐range electron transfer properties of both single‐ and double‐ stranded short synthetic DNA and DNA plasmids. Single mismatch electrochemical detection protocol of non‐labelled short synthetic DNA, without heating or probe labelling, in a 10 minutes protocol, was in fine performed.
Maximization of piezoelectric vibration energy harvesting of vehicle based on double‐gear drive
Authors: Li Yang , Xie Changjun , Quan Shuhai , Li Wenlian , Shi Ying 
Published: July 2019

AbstractA cantilever‐based piezoelectric energy harvesting generator driven by an automatically ‐switchable double‐gear is designed, to assemble the linear‐to‐rotary motion translation and achieve a continuous recovery efficiently by piecewise harvesting. On the basis of identifying the road tolerance grade, an investigation is conducted into the relationship between the gear pitch, vehicle speeds and the energy. A value‐taking method and switching principle are proposed for the gear pitch of the double‐gear under comprehensive driving conditions. According to the tests of two road sections, the results of the simulations show that the automatically ‐switchable double gear can maximize the piezoelectric vibration energy harvesting efficiency under comprehensive driving conditions and road surface with varying tolerances. Meanwhile, results concerning the device experiments indicate that the harvesting efficiency could be improved by 131.66% (76.70%) and 9.37% (22.38%) compared with the single gear with its pitch of 3 mm and 10 mm, respectively.
Genomics of rapid ecological divergence and parallel adaptation in four tidal marsh sparrows
Authors: Walsh Jennifer , Benham Phred M. , Deane-Coe Petra E. , Arcese Peter , Butcher Bronwyn G. , Chan Yvonne L. , Cheviron Zachary A. , Elphick Chris S. , Kovach Adrienne I. , Olsen Brian J. , Shriver W. Gregory , Winder Virginia L. , Lovette Irby J. 
Published: July 2019

AbstractTheory suggests that different taxa having colonized a similar, challenging environment will show parallel or lineage-specific adaptations to shared selection pressures, but empirical examples of parallel evolution in independent taxa are exceedingly rare. We employed comparative genomics to identify parallel and lineage-specific responses to selection within and among four species of North American sparrows that represent four independent, post-Pleistocene colonization events by an ancestral, upland subspecies and a derived salt marsh specialist. We identified multiple cases of parallel adaptation in these independent comparisons following salt marsh colonization, including selection of 12 candidate genes linked to osmoregulation. In addition to detecting shared genetic targets of selection across multiple comparisons, we found many novel, species-specific signatures of selection, including evidence of selection of loci associated with both physiological and behavioral mechanisms of osmoregulation. Demographic reconstructions of all four species highlighted their recent divergence and small effective population sizes, as expected given their rapid radiation into saline environments. Our results highlight the interplay of both shared and lineage-specific selection pressures in the colonization of a biotically and abiotically challenging habitat and confirm theoretical expectations that steep environmental clines can drive repeated and rapid evolutionary diversification in birds.
Lactobacillus paracasei Jlus66 extenuate oxidative stress and inflammation via regulation of intestinal flora in rats with non alcoholic fatty liver disease
Authors: Wang Wei , Li Qian , Chai Wenhui , Sun Chunyan , Zhang Tiehua , Zhao Changhui , Yuan Yuan , Wang Xinyu , Liu Huiqin , Ye Haiqing 
Published: July 2019

AbstractThe nonalcoholic fatty liver disease (NAFLD) is a progressive liver disease that affects the health of people in an increasing rate. In the current research, we investigated the beneficial effect of a novel probiotic strain L. paracasei Jlus66 (Jlus66) on rats with high‐fat diet (HFD)‐induced NAFLD. The intestinal flora of rats was analyzed based on V3‐V4 region 16S rDNA sequencing. Moreover, we measured the oxidative stress and inflammation factors in the liver using commercial ELISA kit, and the lipopolysaccharide (LPS) in serum with chromogenic end‐point tachypheus amebocyte lysate. Compared with the HFD‐induced group, Jlus66 treatment significantly decreased the malondialdehyde (MDA) level in the serum (p < 0.05). Additionally, Jlus66 significantly enhanced the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH‐Px) in the liver and serum (p < 0.05). Jlus66 administration also reduced the levels of tumor necrosis factor (TNF‐α) and interleukin‐6 (IL‐6), and inversely increased the interleukin‐10 (IL‐10) level in serum (p < 0.05). Intestinal flora analysis results showed that Jlus66 can improve intestinal flora structure by increasing the abundance of gram‐positive flora such as Firmicutes, and decreasing gram‐negative flora such as Bacteroidetes, Proteobacteria, and Fusobacteria, and then reduced LPS concentration in the serum. So we concluded that Jlus66 can improve NAFLD by modulating the intestinal flora and followed reduction of oxidative stress (OxS) and inflammation.
Development of quantitative metrics of plume migration at geologic CO2 storage sites
Authors: Harp Dylan , Onishi Tsubasa , Chu Shaoping , Chen Bailian , Pawar Rajesh 
Published: July 2019

AbstractWe develop plume migration metrics based on spatial moment analysis methods that quantify the spatio‐temporal evolution of plumes at geologic CO2 storage sites. The metrics are generalized to handle any 3‐D scalar attribute field values. Within the geologic CO2 storage context, these can be parameters such as CO2 saturation, effective pressure, overpressure, dissolved CO2 concentration, total dissolved solids, pH, and other attributes that are critical for assessing risks. The metrics are comprehensive in that they can effectively handle and account for complex continuous and discontinuous plumes and intra‐plume migration. We demonstrate the metrics on simulated CO2 plumes injected into flat and tilted reservoirs with homogeneous and heterogeneous permeability fields. Using these idealized reservoir scenarios, we demonstrate the information that the metrics extract, showing that the metrics elucidate nuances in plume migration not apparent by standard approaches to the scalar fields values. Published 2019. This article is a U.S. Government work and is in the public domain in the USA.
The Roots of Bio‐Molecular Simulation: The Eight‐Week CECAM Workshop ‘Models for Protein Dynamics’ of 1976
Authors: van Gunsteren Wilfred F. 
Published: July 2019

AbstractThis year, the Centre Européen de Calcul Atomique et Moléculaire (CECAM) celebrates its 50‐th anniversary. Founded in 1969 in Orsay near Paris, it later moved to Lyon and in 2008 to Lausanne. It is an organization devoted to the promotion of fundamental research on advanced computational methods and their application in condensed matter science. Its main vehicle to this end is the organization of workshops. The key role of an eight‐week workshop held forty‐three years ago, characterized by an open exchange of scientific ideas and a foresight regarding the topics relevant to a proper dynamic simulation of bio‐molecules such as proteins, is remembered, together with the issues discussed at the time. These are still relevant today.
A Probabilistic Co-Occurrence Approach for Estimating Likelihood of Spatial Overlap Between Listed Species Distribution and Pesticide Use Patterns
Authors: Richardson Leif , Bang JiSu , Budreski Katherine , Dunne Jonnie , Winchell Michael , Brain Richard A , Feken Max 
Published: July 2019

Abstract
Characterizing potential spatial overlap between federally threatened and endangered (“listed”) species distributions and registered pesticide use patterns is important for accurate risk assessment of threatened and endangered species. Because accurate range information for such rare species is often limited and agricultural pesticide use patterns are dynamic, simple spatial co-occurrence methods may overestimate or underestimate overlap and result in decisions that benefit neither listed species nor the regulatory process. Here, we demonstrate a new method of co-occurrence analysis that employs probability theory to estimate spatial distribution of rare species populations and areas of pesticide use to determine the likelihood of potential exposure. Specifically, we 1) describe a probabilistic method to estimate pesticide use based on crop production patterns; 2) construct species distribution models for 2 listed insect species whose ranges were previously incompletely described, the rusty-patched bumble bee (Bombus affinis) and the Poweshiek skipperling (Oarisma poweshiek); and 3) develop a probabilistic co-occurrence methodology and assessment framework. Using the principles of the Bayes' theorem, we constructed probabilistic spatial models of pesticide use areas by integrating information from land-cover spatial data, agriculture statistics, and remote-sensing data. We used maximum entropy methods to build species distribution models for 2 listed insects based on species collection and observation records and predictor variables relevant to the species' biogeography and natural history. We further developed novel methods for refinement of these models at spatial scales relevant to US Fish and Wildlife Service (FWS) regulatory priorities (e.g., critical habitat areas). Integrating both probabilistic assessments and focusing on USFWS priority management areas, we demonstrate that spatial overlap (i.e., potential for exposure) is not deterministic but instead a function of both species distribution and land use patterns. Our work serves as a framework to enhance the accuracy and efficiency of threatened and endangered species assessments using a data-driven likelihood analysis of species co-occurrence. Integr Environ Assess Manag 2019;00:1–12. © 2019 SETAC
Evaluation of neuroprotective activity of digoxin and semisynthetic derivatives against partial chemical ischemia
Authors: de Souza Gonçalves Bruno , de Moura Valadares Jéssica M. , Alves Silmara L. G. , Silva Simone C. , Rangel Luciana P. , Cortes Vanessa F. , Villar José A. F. P. , Barbosa Leandro A. , de Lima Santos Hérica 
Published: July 2019

AbstractRecently, cardiotonic steroids (CTS) have been shown to lead to the activation of Na,K‐ATPase at low concentrations in brain, promoting neuroprotection against ischemia. We report here the results of the use of digoxin and its semisynthetic derivatives BD‐14, BD‐15, and BD‐16 against partial chemical ischemic induction followed by reperfusion in murine neuroblastoma cells neuro‐2a (N2a). For chemical ischemic induction, sodium azide (5 mM) was used for 5 hours, and then reperfusion was induced for 24 hours. Na,K‐ATPase activity and protein levels were analyzed in membrane preparation of N2a cells pretreated with the compounds (150 nM), in the controls and in induced chemical ischemia. In the Na,K‐ATPase activity and protein levels assays, the steroids digoxin and BD‐15 demonstrated a capacity to modulate the activity of the enzyme directly, increasing its levels of expression and activity. Oxidative parameters, such as superoxide dismutase (SOD) activity, lipid peroxidation (thiobarbituric acid reactive substance), glutathione peroxidase (GPx), glutathione (GSH) levels, hydrogen peroxide content, and the amount of free radicals (reactive oxygen species) during induced chemical ischemia were also evaluated. Regarding the redox state, lipid peroxidation, hydrogen peroxide content, and GPx activity, we have observed an increase in the chemical ischemic group, and a reduction in the groups treated with CTS. SOD activity increased in all treated groups when compared to control and GSH levels decreased when treated with sodium azide and did not change with CTS treatments. Regarding the lipid profile, we saw a decrease in the content of phospholipids and cholesterol in the chemical ischemic group, and an increase in the groups treated with CTS. In conclusion, the compounds used in this study demonstrate promising results, since they appear to promote neuroprotection in cells exposed to chemical ischemia.
Molecular docking studies of chloroquine and its derivatives against P23pro‐zbd domain of chikungunya virus: Implication in designing of novel therapeutic strategies
Authors: Kumar Maneesh , Topno Roshan Kamal , Dikhit Manas Ranjan , Bhawana  , Sahoo Ganesh Chandra , Madhukar Major , Pandey Krishna , Das Pradeep 
Published: July 2019

AbstractThe arthropod‐transmitted chikungunya virus has emerged as an epidemic menace that causes debilitating polyarthritis. With this life‐threatening impact on humans, the possible treatment requires to cure the viral infectivity. But, devoid of any vaccine against the chikungunya virus (CHIKV), there is a need to develop a novel chemotherapeutic strategy to treat this noxious infection. CHIKV carries highly compact P23pro‐zbd structure that possesses potential RNA‐binding surface domains which extremely influences the use of RNA template during genome replication at the time of infection and pathogenesis. Therefore, computational approaches were used to explore the novel small molecule inhibitors targeting P23pro‐zbd domain. The tertiary structure was modeled and optimized using in silico approaches. The results obtained from PROCHECK (93.1% residues in favored regions), ERRAT (87.480 overall model quality) and ProSA (Z‐score: −11.72) revealed the reliability of the proposed model. Interestingly, a previously reported inhibitor, chloroquine possesses good binding affinities with the target domain. In‐depth analysis revealed that chloroquine derivatives such as didesethyl chloroquine hydroxyacetamide, cletoquine, hydroxychloroquine exhibited a better binding affinity. Notably, MD simulation analysis exhibited that Thr1312, Ala1355, Ala1356, Asn1357, Asp1364, Val1366, Cys1367, Ala1401, Gly1403, Ser1443, Tyr1444, Gly1445, Asn1459, and Thr1463 residues are the key amino acid responsible for stable ligand‐protein interaction. The results obtained from this study provide new insights and advances the understanding to develop a new approach to consider effective and novel drug against chikungunya. However, a detailed in vivo study is required to explore its drug likeliness against this life‐threatening disease.
LncRNA SNHG15 promotes hepatocellular carcinoma progression by sponging miR‐141‐3p
Authors: Ye Junfeng , Tan Ludong , Fu Yu , Xu Hongji , Wen Lijia , Deng Yu , Liu Kai 
Published: July 2019

AbstractSmall nucleolar RNA host gene 15 (SNHG15) is a long noncoding RNA (lncRNA), which promotes progression of multiple cancers. Its specific function in hepatocellular carcinoma (HCC), however, is uncertain. The aims of our study were, therefore, to explore the role of SNHG15 in HCC. SNHG15 and miR‐141‐3p expression were assessed via quantitative real‐time PCR (qRT‐PCR) in 58 paired HCC samples and adjacent matched adjacent normal tissues. CCK‐8 assay, flow cytometric examination, and wound healing/invasion assays were used to respectively assess how SNHG15 influences cell proliferation, the cell cycle, and the migratory and invasive potential of HCC cells. MicroRNA (miRNAs) that targeted SNHG15 was screened by Starbase2.0 and identified by RNA immunoprecipitation and luciferase reporter assays. SNHG15 expression was markedly increased, whereas miR‐141‐3p expression was substantially reduced in HCC cells and tissue samples relative to normal controls. When SNHG15 was knocked down, this resulted in a significant disruption to the proliferation, as well as the invasive and migratory ability of these HCC cells. miR‐141‐3p was also found to be an SNHG15 target in HCC cells. Furthermore, miR‐141‐3p inhibitor partially reversed the observed SNHG15 depletion‐mediated reduction in HCC proliferation, migration, and invasion. By repressing miR‐141‐3p, SNHG15 could modulate zinc finger E‐box binding homeobox 2 (ZEB2) and E2F transcription factor 3 (E2F3) expression, both of which are miR‐141‐3p targets. These finding suggested that SNHG15 promoted HCC progression via negative regulation of miR‐141‐3p, thus identifying a potential novel HCC treatment pathway.
Integrated mRNA‐Seq and miRNA‐Seq analysis of PLCγ2‐overexpressing hepatocarcinoma cells and identification of the associated miRNA‐mRNA network
Authors: Chen Xiaoguang , Zhu Xuemin , Wei Zhiguo , Lv Qiongxia 
Published: July 2019

AbstractOur previous study has discovered the positive effect of phospholipase Cγ 2 (PLCγ2) on the growth of hepatocarcinoma cells; however, the underlying mechanism is far from being understood. For this reason, this study attempts to identify the differently expressed microRNAs (miRNAs) and messenger RNAs (mRNAs) in PLCγ2‐overexpressing hepatocarcinoma cells. The results showed that totally 596 differently‐expressed genes (DEGs) were identified in PLCγ2‐expressed cells, including 314 upregulated and 282 downregulated ones; according to gene ontology analysis, these DEGs were involved in different cellular processes. Concurrently, 34 differently‐expressed miRNAs (DEMs) were also detected in PLCγ2‐expressing hepatocarcinoma cells. Moreover, the integrative analysis of miRNA and mRNA expression profiles identified the potential regulatory network linked to hepatocarcinoma‐related biological processes, including metabolic activity, gene expression, cell cycle, cell migration, and so on. To our knowledge, it is the first study on the effect of PLCγ2 on miRNA and mRNA expressions in hepatocarcinoma cells, and the findings provide new insights into the mechanism supporting the growth‐promoting effect of PLCγ2 in hepatocarcinoma cells.