bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of the ICF‐Incontinence Assessment Form (ICF‐IAF) to identify problems and resources for planning and evaluation of interventions using the Classification of Functioning, Disability and Health of the World Health Organization: Preliminary study
Authors: König Irene , Kuhn Manuel , Radlinger Lorenz , Koehler Barbara 
Published: February 2019

AbstractAimsCurrent level‐A recommended questionnaires that provide a valid assessment of urinary incontinence (UI) and/or faecal incontinence (FI), but do not cover all aspects of the burden of the disease in the sense of the bio‐psycho‐social model of the World Health Organization's International Classification of Functioning, Disability and Health (ICF). Therefore, an ICF oriented questionnaire for patients with UI and/or FI to assess treatment barriers (problems) as well as positive influencing factors (resources), the “ICF‐Incontinence Assessment Form” will be developed. The aim of this preliminary study was (a) to identify problems and resources of UI and/or FI in current level‐A recommended questionnaires and (b) to evaluate problems and resources of patients with UI and/or FI from the perspective of experienced physiotherapists.Methods(a) Current level‐A recommended questionnaires for UI and/or FI were analyzed. Questions were linked to the ICF categories. (b) A 3‐round Delphi technique survey among 262 physiotherapists from five German‐speaking countries was used to evaluate patients' problems and resources relevant to physiotherapy.Results(a) A total of 27 identified questionnaires were linked to 110 ICF categories. No positive influencing factors could be identified. (b) After Delphi‐round 3, 110 categories of problems and 71 resources were identified.ConclusionSince there is a lack of resource items in the present validated questionnaires, there is a need for a short, practical questionnaire to assess and monitor both, problems and resources of patients with UI and/or FI.
Fire truck relocation during major incidents
Authors: Usanov Dmitrii , Guido Legemaate G.A. , van de Ven Peter M. , van der Mei Rob D. 
Published: February 2019

AbstractThe effectiveness of a fire department is largely determined by its ability to respond to incidents in a timely manner. To do so, fire departments typically have fire stations spread evenly across the region, and dispatch the closest truck(s) whenever a new incident occurs. However, large gaps in coverage may arise in the case of a major incident that requires many nearby fire trucks over a long period of time, substantially increasing response times for emergencies that occur subsequently. We propose a heuristic for relocating idle trucks during a major incident in order to retain good coverage. This is done by solving a mathematical program that takes into account the location of the available fire trucks and the historic spatial distribution of incidents. This heuristic allows the user to balance the coverage and the number of truck movements. Using extensive simulation experiments we test the heuristic for the operations of the Fire Department of Amsterdam‐Amstelland, and compare it against three other benchmark strategies in a simulation fitted using 10 years of historical data. We demonstrate substantial improvement over the current relocation policy, and show that not relocating during major incidents may lead to a significant decrease in performance.
Log Often, Lose More: Electronic Dietary Self‐Monitoring for Weight Loss
Authors: Harvey Jean , Krukowski Rebecca , Priest Jeff , West Delia 
Published: February 2019

ObjectiveDietary self‐monitoring is consistently related to both short‐ and long‐term weight loss. The purpose of this study was to quantify the time spent and the daily frequency of self‐monitoring necessary for weight‐loss success.MethodsParticipants in a 24‐week, online, behavioral weight‐control intervention recorded daily dietary intake using a Web‐based dietary analysis program. Time spent self‐monitoring and frequency of dietary journal page access were captured. Weight loss (kilograms) and the proportion of participants losing ≥ 5% and ≥ 10% of baseline weight were assessed at 6 months.ResultsParticipants (n = 142; BMI 35.8 kg/m2; 90.8% female; 23.2% African American) spent an average of 23.2 minutes per day self‐monitoring in month 1 and 14.6 minutes in month 6. For those still recording any minutes self‐monitoring by month 6 (65.5%), there were no significant differences in time spent based on weight loss; however, those losing either ≥ 5% or ≥ 10% logged in to the journal Web page significantly more times per day (1.6 vs. 2.4, P < 0.001 for < 5% vs. ≥ 5%; 1.7 vs. 2.7, P < 0.001 for < 10% vs. ≥ 10%).ConclusionsThe frequency of self‐monitoring is significantly related to weight loss, with the time needed to be successful diminishing during the intervention.
Characterizing adolescents with heavy menstrual bleeding and generalized joint hypermobility
Authors: Kendel Nicole E. , Haamid Fareeda W. , Christian‐Rancy Myra , O'Brien Sarah H. 
Published: February 2019

AbstractPatients with generalized joint hypermobility (JHM) may experience excessive bruising/bleeding, with heavy menstrual bleeding (HMB) commonly reported. We performed a retrospective review of 30 adolescents seen in a Young Women's Hematology Clinic with both HMB and JHM. We found that (1) a significant delay (mean 36 months, range 5–72) occurred between menarche and referral to specialty care, (2) HMB had moderate to severe impact on school and physical activities in 60% of patients, and (3) most patients (68%) required escalation of their initial therapy. We suggest providers consider JHM as a risk factor for a more complex clinical course.
An injectable and self‐healing novel chitosan hydrogel with low adamantane substitution degree
Authors: Liu Cheng , Yang Liquan , Qiao Liyuan , Liu Chengde , Zhang Manxia , Jian Xigao 
Published: February 2019

AbstractChitosan (CS) is a semi‐natural polymer with supreme biological function, while the strong interchain hydrogen bonds cause poor water solubility and limit its broader use. To break the semi‐rigid structure of CS, a kind of CS modified by adamantane (AD‐CS) was successfully synthesized by amidation reaction with 1‐(3‐dimethylaminopropyl)‐3‐ethylcarbodiimide hydrochloride as catalyst. The chemical structure of AD‐CS was characterized by Fourier transform infrared spectroscopy and 1H NMR. The AD substitution degree of CS is around 2%, calculated by 1H NMR. A soft and transparent hydrogel composed of hydrogen bonds was obtained directly by simply adding a certain amount of water under mild conditions. Rheological measurements were carried out to research the mechanism of hydrogel formation by measuring the influence of different additives and conditions on the AD‐CS hydrogel. Reinforced hydrogels were prepared by freezing and thawing. The mechanical strength and self‐healing property of reinforced and pristine hydrogels were assessed with an oscillatory rheometer. The modulus of the reinforced hydrogel was obviously enhanced without much loss of self‐healing property. Other properties such as adhesion, injectability and temperature response were also studied. These injectable and self‐healing hydrogels show potential value in medical care. Additionally, this is a new method to design CS hydrogels with their original interchain hydrogen bonds. © 2019 Society of Chemical Industry
Optimization of colistin dosing regimen for cystic fibrosis patients with chronic Pseudomonas aeruginosa biofilm lung infections
Authors: Hengzhuang Wang , Green Kent , Pressler Tacjana , Skov Marianne , Katzenstein Terese L , Wu Xiaojie , Høiby Niels 
Published: February 2019

AbstractObjectiveThe present study was performed to explore dosing regimens of colistin in patients of cystic fibrosis (CF) with Pseudomonas aeruginosa chronic biofilm lung infection.MethodsTen CF patients were involved. One dose colistimethate sodium (CMS) of 6 MIU (million international units) and 9 MIU were administered by intravenous infusion over 45 and 90 min. Venous blood was collected at different time points after the infusion of CMS. Pharmacokinetic parameters of colistin were calculated. Minimum inhibitory concentration for planktonic P. aeruginosa, minimum biofilm inhibitory concentration and minimum biofilm eradication concentration of P. aeruginosa were determined. Monte Carlo simulation was performed to determine the clinical probability of target attainment of different dosing regimens of colistin in CF patients.ResultsFor 90 min (6 MIU), 45 min (6 MIU), and 45 min (9 MIU) intravenous infusion of colistin, Cmax was 8.9 ± 1.8, 15 ± 5.5, and 31.7 ± 5.3 μg/mL, respectively; Tmax was 1.2 ± 0.4, 0.7 ± 0.2, and 0.8 ± 0.2 h, respectively; AUCtot were 31 ± 3.8, 34 ± 10, and 135 ± 31mg · h/L, respectively; t1/2 was 2.1 ± 0.4, 2 ± 0.3, and 3.3 ± 0.4 h, respectively. MBIC and MBEC of colistin on biofilms at 24 h period treatment were 16‐128 μg/mL for non‐mucoid and mucoid biofilms of P. aeruginosa. For 90 min (6 MIU), 45 min (6 MIU) and 45 min iv infusion (9 MIU) with one dose colistin, PTA was 49.8%, 53.8%, 99.4% for planktonic infection, and 11.3%, 14.6%, 65.3%, respectively for biofilm infection.Conclusionscolistin treatment using 45 min iv infusion is better than 90 min iv infusion in this study. Colistin dosage of 9 MIU is better than 6 MIU on both planktonic and biofilm infections of P. aeruginosa in this study.
PEOPLE: PatiEnt prOstate samPLes for rEsearch, a tissue collection pathway utilizing magnetic resonance imaging data to target tumor and benign tissue in fresh radical prostatectomy specimens
Authors: Heavey Susan , Costa Helena , Pye Hayley , Burt Emma C. , Jenkinson Sophia , Lewis Georgina‐Rose , Bosshard‐Carter Leticia , Watson Fran , Jameson Charles , Ratynska Marzena , Ben‐Salha Imen , Haider Aiman , Johnston Edward W. , Feber Andrew , Shaw Greg , Sridhar Ashwin , Nathan Senthil , Rajan Prabhakar , Briggs Timothy P. , Sooriakumaran Prasanna , Kelly John D. , Freeman Alex , Whitaker Hayley C. 
Published: February 2019

AbstractBackgroundOver 1 million men are diagnosed with prostate cancer each year worldwide, with a wide range of research programs requiring access to patient tissue samples for development of improved diagnoses and treatments. A random sampling of prostate tissue is sufficient for certain research studies; however, there is growing research need to target areas of the aggressive tumor as fresh tissue. Here we set out to develop a new pathway “PEOPLE: PatiEnt prOstate samPLes for rEsearch” to collect high‐quality fresh tissue for research use, using magnetic resonance imaging (MRI) to target areas of tumor and benign tissue.MethodsProstate tissue was sampled following robotic radical prostatectomy, using MRI data to target areas of benign and tumor tissue. Initially, 25 cases were sampled using MRI information from clinical notes. A further 59 cases were sampled using an optimized method that included specific MRI measurements of tumor location along with additional exclusion criteria. All cases were reviewed in batches with detailed clinical and histopathological data recorded. For one subset of samples, DNA was extracted and underwent quality control. Ex vivo culture was carried out using the gelatin sponge method for an additional subset.ResultsTumor was successfully fully or partially targeted in 64% of the initial cohort and 70% of the optimized cohort. DNA of high quality and concentration was isolated from 39 tumor samples, and ex vivo culture was successfully carried out in three cases with tissue morphology, proliferation, and apoptosis remaining comparable before and after 72 hours culture.ConclusionHere we report initial data from the PEOPLE pathway; using a method for targeting areas of tumor within prostate samples using MRI. This method operates alongside the standard clinical pathway and minimizes additional input from surgical, radiological, and pathological teams, while preserving surgical margins and diagnostic tissue.
Effects of PI(4,5)P2 concentration on the F‐BAR domain membrane binding as revealed by coarse‐grained simulations
Authors: Stanishneva‐Konovalova Tatiana B. , Sokolova Olga S. 
Published: February 2019

AbstractBin/Amphyphysin/Rvs (BAR) domain proteins form a key link between membrane remodeling and cytoskeleton dynamics. They are dimers that bind to membranes via electrostatic interactions with different preferences toward negatively charged lipids. In the present article, we examine the interactions of the F‐BAR domain of nervous wreck (Nwk) with phosphatidylinositol 4,5‐bisphosphate (PI(4,5)P2)‐containing membranes using coarse‐grained molecular dynamics. We demonstrated PI(4,5)P2 concentration effects, identified the sequence of events that underlies the protein binding and identified amino acids involved in protein–lipid interactions. Our simulations point out the primary role of the basic stretch at the tips of the dimer, which anchors the protein to the membrane and initiates the binding process. When the PI(4,5)P2 concentration is high, the protein stably associates with the membrane by its concave surface or by the opposite side. At low PI(4,5)P2 concentration, the former orientation becomes more favorable; also a state with only one tip bound is observed, due to the weaker attachment and more pronounced association/dissociation events. Our results provide a theoretical model that describes the lipid‐binding behavior of Nwk observed in vitro.
Compounds from the root of Pueraria peduncularis (Grah. ex Benth.) Benth. and their antimicrobial effects
Authors: Chen Huabao , Zhao Xiaomin , Lv Tianxing , Qiu Xiaoyan , Luo Liya , Zhang Min , Yang Hui , Chang Xiaoli , Li Peili , Wu ChuanLei , Xie Deshan , Qian Yong , Yang Chunping 
Published: February 2019

ABSTRACTBACKGROUNDPueraria peduncularis belongs to the genus Pueraria DC, and has a wide range of medical and agricultural activities. Previous studies have shown that P. peduncularis extracts have broad bioactivities against phytopathogens. In this paper, we systematically studied the fungicidal activity of root methanol extracts and further isolated the active compounds.RESULTSThe root methanol extract inhibited the mycelial growth of the five tested phytopathogens to different degrees. Among these phytopathogens, the inhibitory effect was greatest against R. solani, with an EC50 value of 324.72 mg L−1. Eight compounds were subsequently isolated and identified from P. peduncularis. Among them, puercarpan A and medicarpin showed strong fungicidal activity, with MIC values against Rhizoctonia solani of 1.6 and 6.25 mg L−1, respectively. Puercarpan A is a new compound, and its structure was established as (6aR,6bS,11aR)‐6b‐hydroxy‐3‐methoxypterocarpan‐10‐ene‐7‐one.CONCLUSIONThe P. peduncularis extracts exhibit high antimicrobial activity against R. solani and have great potential value of P. peduncularis as a fungicide. © 2019 Society of Chemical Industry
Rapid screening methods for yeast sub‐metabolome analysis with a high‐resolution ion mobility quadrupole time‐of‐flight mass spectrometer
Authors: Mairinger Teresa , Kurulugama Ruwan , Causon Tim J. , Stafford George , Fjeldsted John , Hann Stephan 
Published: February 2019

RationaleThe wide chemical diversity and complex matrices inherent to metabolomics still pose a challenge to current analytical approaches for metabolite screening. Although dedicated front‐end separation techniques combined with high‐resolution mass spectrometry set the benchmark from an analytical point of view, the increasing number of samples and sample complexity demand for a compromise in terms of selectivity, sensitivity and high‐throughput analyses.MethodsPrior to low‐field drift tube ion mobility (IM) separation and quadrupole time‐of‐flight mass spectrometry (QTOFMS) detection, rapid ultrahigh‐performance liquid chromatography separation was used for analysis of different concentration levels of dansylated metabolites present in a yeast cell extract. For identity confirmation of metabolites at the MS2 level, an alternating frame approach was chosen and two different strategies were tested: a data‐independent all‐ions acquisition and a quadrupole broad band isolation (Q‐BBI) directed by IM drift separation.ResultsFor Q‐BBI analysis, the broad mass range isolation was successfully optimized in accordance with the distinctive drift time to m/z correlation of the dansyl derivatives. To guarantee comprehensive sampling, a broad mass isolation window of 70 Da was employed. Fragmentation was performed via collision‐induced dissociation, applying a collision energy ramp optimized for the dansyl derivatives. Both approaches were studied in terms of linear dynamic range and repeatability employing ethanolic extracts of Pichia pastoris spiked with 1 μM metabolite mixture. Example data obtained for histidine and glycine showed that drift time precision (<0.01 to 0.3% RSD, n = 5) compared very well with the data reported in an earlier IM‐TOFMS‐based study.ConclusionsChimeric mass spectra, inherent to data‐independent analysis approaches, are reduced when using a drift time directed Q‐BBI approach. Additionally, an improved linear dynamic working range was observed, representing, together with a rapid front‐end separation, a powerful approach for metabolite screening.
Ultrasound‐Mediated Synthesis of Novel α‐Aminophosphonates Using Graphene Nanosheets‐Silver Nanoparticles (GNS‐AgNPs) as a Recyclable Heterogeneous Catalyst
Authors: Rasal Sarika A , Tamore Milind S , Shimpi Navinchandra G 
Published: February 2019

AbstractA series of novel α‐aminophosphonate derivatives were synthesized via simple and an efficient method from the three‐component condensation reaction of 5‐aminoindan or 3,4‐(methylenedioxy)aniline, aromatic aldehydes and diethyl phosphite by employing graphene nanosheets‐silver nanoparticles (GNS‐AgNPs) as catalyst under ultrasonication and solvent‐free condition. GNS‐AgNPs was prepared in situ by simultaneous reduction of graphene oxide (GO) and silver nitrate (AgNO3) using sodium borohydride (NaBH4) as a reducing agent. The surface characterization of GNS‐AgNPs was done using X‐ray diffraction (XRD), Fourier transform infrared (FTIR), UV–Vis spectroscopy (UV–Vis), Thermogravimetric analysis (TGA), Raman spectroscopy and Field emission scanning electron microscopy (FE‐SEM). The GNS‐AgNPs are recyclable up to fifth run with minimal loss of its activity. Convenient operational simplicity, mild conditions, excellent yields of products, consistent performance and the reusability of catalyst makes this protocol feasible and attractive.
Tailoring Catalytic Properties of CuMgAl Hydrotalcites for Selective hydrogenolysis of Cellulose
Authors: Xiao Zihui , Ma Pengfei , Jin Shaohua , Liang Changhai 
Published: February 2019

AbstractThe structure and catalytic properties of CuMgAl ternary hydrotalcites were tailored by adjusting MII/MIII molar ratios. Due to the Jahn‐Teller distortion effect, co‐crystallized impurity phase assigned to Cu2(NO3)(OH)3 was formed for the catalyst with high MII/MIII molar ratio. The formation of impurity phase resulted in the significant change of morphologies and low dispersion of copper species in the hydrotalcites. Nevertheless, the corresponding catalyst exhibited great catalytic proformance in term of ethylene glycol (EG) selectivity for the hydrogenolysis of cellulose. Besides, higher activity was obtained over the catalysts with higher content of Mg due to an increase of the alkalinity.
Advances of Proteomics in Novel PTM Discovery: Applications in Cancer Therapy
Authors: Wang Yang , Zhang Jing , Li Bin , He Qing‐Yu 
Published: February 2019

AbstractProtein posttranslational modification (PTM) is a critical mechanism to enhance the diversity of protein species and functions in organisms. Currently, many different PTMs are discovered and found to be involved in a wide range of cellular processes. The aberrant regulation of PTM dynamics is reported to contribute to cancer development. Phosphorylation and acetylation are the best studied PTMs, and the application of high‐resolution mass spectrometry‐based proteomics and specific antibody‐based enrichment technologies significantly promotes novel PTM discovery. This review discusses methods for global PTM identification, including antibody‐based enrichment and chemical probe‐based identification. Several acylations, such as propionylation, butyrylation, succinylation, malonylation, and crotonylation, are recently found to influence both histone and nonhistone proteins and to be intimately linked with the progression of cancer. It is proposed that targeting novel PTM dynamics by pharmacological inhibitors has great potential for cancer therapy.
Somatic MIWI2 Hinders Direct Lineage Reprogramming From Fibroblast to Hepatocyte
Authors: Shi Xiaojie , Xiao Zipei , Zonta Francesco , Wang Wei , Wan Yue , Li Yu , Wang Nan , Kuang Yuanyuan , Du Mingjuan , Dong Jian , Wang Ju , Yang Guang 
Published: February 2019

Abstract
Remodeling of the gene regulatory network in cells is believed to be a prerequisite for their lineage reprogramming. However, its key regulatory factors are not yet elucidated. In this article, we investigate the role of PIWI proteins and provide evidence that one of them, MIWI2, is elicited during transdifferentiation of fibroblasts into hepatocyte-like cells. In coincidence with the peak expression of MIWI2, we identified the appearance of a unique intermediate epigenetic state characterized by a specific Piwi-interacting RNA (piRNA) profile consisting of 219 novel sequences. Knockout of MIWI2 greatly improved the formation of the induced hepatocytes, whereas overexpression of exogenous MIWI2 completely abolished the stimulated effect. A bioinformatics analysis of piRNA interaction network, followed by experimental validation, revealed the Notch signaling pathway as one of the immediate effectors of MIWI2. Altogether, our results show for the first time that temporal expression of MIWI2 contributes negatively to cell plasticity not only in germline, but also in developed cells, such as mouse fibroblasts. Stem Cells 2019;37:803–812
Regorefenib induces extrinsic/intrinsic apoptosis and inhibits MAPK/NF‐κB‐modulated tumor progression in bladder cancer in vitro and in vivo
Authors: Chiang Chih‐Hung , Chung Jing‐Gung , Hsu Fei‐Ting 
Published: February 2019

AbstractThe aim of the present study is to investigate anticancer effect and mechanism of regorafenib in bladder cancer in vitro and in vivo. Human bladder cancer TSGH 8301 cells were treated with regorafenib, NF‐κB, AKT, or mitogen‐activated protein kinase (MAPK) inhibitors for different time. The changes of cell viability, NF‐κB activation, apoptotic signaling transduction, and expression of tumor progression‐associated proteins were evaluated with MTT, NF‐κB reporter gene assay, flow cytometry, and Western blotting assay. TSGH 8301 tumor bearing mice were established and treated with vehicle (140 μL of 0.1% DMSO) or regorafenib (10 mg/kg/day by gavage) for 15 days. The changes of tumor volume, body weight, NF‐κB activation, MAPK activation, and tumor progression‐associated proteins (MMP‐9, XIAP, VEGF, and Cyclin‐D1) after regorafenib treatment were evaluated with digital caliper, digital weight, and ex vivo Western blotting assay. Our results demonstrated NF‐κB activation and protein levels of MMP‐9, XIAP, VEGF, and Cyclin‐D1 were significantly reduced by NF‐κB (QNZ), ERK (PD98059), and P38 (SB203580) inhibitors. Regorafenib also significantly induced extrinsic and intrinsic apoptotic signaling transduction in bladder cancer in vitro. In addition, regorafenib significantly inhibited tumor growth, NF‐κB, p38, ERK activation and expression of tumor progression‐associated proteins in bladder cancer in vitro and in vivo. Taken together, these results proved that regorafenib not only induced apoptosis through extrinsic and intrinsic pathways and but suppressed MAPK/ NF‐κB‐modulated tumor progression in bladder cancer.