bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromatin-dependent regulation of secondary metabolite biosynthesis in fungi: is the picture complete?
Authors: Collemare Jérôme , Seidl Michael F 
Published: June 2019

ABSTRACT
Fungal secondary metabolites are small molecules that exhibit diverse biological activities exploited in medicine, industry and agriculture. Their biosynthesis is governed by co-expressed genes that often co-localize in gene clusters. Most of these secondary metabolite gene clusters are inactive under laboratory conditions, which is due to a tight transcriptional regulation. Modifications of chromatin, the complex of DNA and histone proteins influencing DNA accessibility, play an important role in this regulation. However, tinkering with well-characterised chemical and genetic modifications that affect chromatin alters the expression of only few biosynthetic gene clusters, and thus the regulation of the vast majority of biosynthetic pathways remains enigmatic. In the past, attempts to activate silent gene clusters in fungi mainly focused on histone acetylation and methylation, while in other eukaryotes many other post-translational modifications are involved in transcription regulation. Thus, how chromatin regulates the expression of gene clusters remains a largely unexplored research field. In this review, we argue that focusing on only few well-characterised chromatin modifications is significantly hampering our understanding of the chromatin-based regulation of biosynthetic gene clusters. Research on underexplored chromatin modifications and on the interplay between different modifications is timely to fully explore the largely untapped reservoir of fungal secondary metabolites.
The whole is more than the sum of the parts: establishing an enabling health system environment for reducing acute child malnutrition in a rural South African district
Authors: Schneider Helen , van der Merwe Maria , Marutla Beauty , Cupido Joseph , Kauchali Shuaib 
Published: June 2019

Abstract
There is a gap in understanding of how national commitments to child nutrition are translated into sub-national implementation. This article is a mixed methods case study of a rural South African health district which achieved accelerated declines in morbidity and mortality from severe acute malnutrition (SAM) in young children, following a district health system strengthening (HSS) initiative centred on real-time death reporting, analysis and response. Drawing on routine audit data, the declining trends in under-five admissions and in-hospital mortality for SAM over a 5-year period are presented, comparing the district with two others in the same province. Adapting Gillespie et al.’s typology of ‘enabling environments’ for Maternal and Child Nutrition, and based on 41 in-depth interviews and a follow-up workshop, the article then presents an analysis of how an enabling local health system environment for maternal-child health was established, creating the conditions for achievement of the SAM outcomes. Embedded in supportive policy and processes at national and provincial levels, the district HSS interventions and the manner in which they were implemented produced three kinds of system-level change: knowledge and use of evidence by providers and managers (‘ways of thinking’), leadership, participation and coordination (‘ways of governing’) and inputs and capacity (‘ways of resourcing’). These processes mainstreamed responsibility, deepened accountability and triggered new service delivery and organizational practices and mindsets. The article concludes that it is possible to foster enabling district environments for the prevention and management of acute malnutrition, emphasizing the multilevel and simultaneous nature of system actions, where action on system ‘software’ complements the ‘hardware’ of HSS interventions, and where the whole is more than the sum of the parts.
Comparative spallation performance of silicone versus Tygon extracorporeal circulation tubing
Authors: Ippoliti Francesco , Piscioneri Fernando , Sartini Patrizio , Peruzzi Mariangela , Domenico Marina Di , Dannhauser David , Rossi Domenico , Causa Filippo , Netti Paolo Antonio , Miraldi Fabio , Greco Ernesto , Marullo Antonino , Iaccarino Alessandra , Cavarretta Elena , Zoccai Giuseppe Biondi , Sciarretta Sebastiano , Frati Giacomo 
Published: June 2019

AbstractOBJECTIVESReports ranged from mixed to marginal tubing wear and spallation effects as a complication of roller pumps in cardiopulmonary bypass (CPB). Because the rollers constantly compress part of the tubing, we sought to determine whether circuit materials behave differently under a 3-h simulation of CPB.METHODSTwo different tubing materials (silicone and Tygon) were tested with a customized experimental circuit, designed to allow in vitro simulation of CPB with priming volumes, pressures, revolutions per minute and temperatures equivalent to the clinical scenario. Samples were analysed with optical and field-emission scanning electron microscopy. We collected 200-ml fluid samples at 4 different times: before starting the CPB (T0), when the predicted revolutions per minute corresponded to about 2 min of CPB (T1), at 90 min (T2) and at 180 min (T3). At the end of CPB, we harvested 2 samples of tubing. Lastly, optical investigations and field-emission scanning electron microscopy observations were used for qualitative and quantitative analysis of circulating fragments.RESULTST2 and T3 fluid samples showed more particles than T1 samples. Significant differences in terms of particle numbers were detected: silicone tubing released more fragments per millilitre than Tygon tubing, with both materials releasing particles from 5 to 500 µm. Silicone tubing was associated with a time-dependent increase in small particles released (P = 0.04), whereas this did not apply to large particles or to Tygon tubing. Yet, bootstrap estimates suggested that silicone tubing was associated with the release of more small particles whereas Tygon tubing released more large particles (both P < 0.01). Unlike silicone, Tygon samples taken from the portion of the circuit not subjected to the action of the roller pump did not show any erosion on their surfaces. Samples of both materials taken from the portion subjected to the compression of the roller pump showed signs of significant deterioration.CONCLUSIONSSilicone showed a worse spallation performance than Tygon, thus appearing less safe for more complex surgery of prolonged duration or for patients with a prior cerebral ischaemic event. Additional risk and cost-effectiveness comparisons to determine the potential benefits of one type of tubing material over the other are warranted to further expand our findings.
Updated good practice recommendations for outpatient parenteral antimicrobial therapy (OPAT) in adults and children in the UK
Authors: Chapman Ann L N , Patel Sanjay , Horner Carolyne , Green Helen , Guleri Achyut , Hedderwick Sara , Snape Susan , Statham Julie , Wilson Elizabeth , Gilchrist Mark , Seaton R Andrew 
Published: June 2019

AbstractUK good practice recommendations for outpatient parenteral antimicrobial therapy (OPAT) were published in 2012 and 2015 for adult and paediatric patients, respectively. Here we update the initial good practice recommendations in a combined document based on a further review of the OPAT literature and an extensive consultation process. As with the previous good practice recommendations, these updated recommendations are intended to provide pragmatic guidance for new and established OPAT services across a range of settings and to act as a set of quality indicators for service evaluation and quality improvement.
Genetic parameters for hair characteristics and core body temperature in a multibreed Brahman–Angus herd1
Authors: Sarlo Davila Kaitlyn M , Hamblen Heather , Hansen Peter J , Dikmen Serdal , Oltenacu Pascal A , Mateescu Raluca G 
Published: June 2019

Abstract
Thermal stress in hot humid conditions limits cattle production. The objectives for this study were to estimate genetic parameters for hair characteristics and core body temperature under low and high temperature humidity index (THI) conditions. Hair samples were collected and measured for length and diameter. Core body temperature was measured as vaginal temperature every 15 min over a 5-d period using an iButton temperature measuring device implanted in a blank CIDR in 336 heifers from the University of Florida multibreed herd (ranging from 100% Angus to 100% Brahman). Restricted maximum likelihood procedures were used to estimate heritabilities from multiple bivariate animal models using the WOMBAT program. Estimates of heritability for hair diameter, undercoat length, topcoat length, body temperature under low THI conditions, and body temperature under high THI conditions were 0.50, 0.67, 0.42, 0.32, and 0.26, respectively. The genetic parameters estimated in this study indicate a large, exploitable genetic variance which can be selected upon to improve tolerance in cattle. Breed effects for differing compositions of Brahman and Angus were also estimated. As Brahman breed composition increased by 25% undercoat length, topcoat length, body temperature under low THI conditions, and body temperature under high THI conditions decreased by 1.32 mm, 2.94 mm, 0.11 °C, and 0.14 °C, respectively. Under both low and high THI conditions, cattle with 25% Brahman breed composition or greater maintained a significantly lower body temperature than the 100% Angus breed group. The incorporation of Brahman germplasm is recommended for herds that often experience heat stress conditions in order to increase resilience to heat stress.
Risk factor for wound complications following wide resection of soft tissue sarcoma in the adductor compartment of the thigh
Authors: Kito Munehisa , Ae Keisuke , Koyanagi Hirotaka , Gokita Tabu , Furuoka Hideto , Okamoto Masanori , Tanaka Atsushi , Suzuki Shuichiro , Takazawa Akira , Aoki Kaoru , Yoshimura Yasuo 
Published: June 2019

AbstractBackgroundThe purpose of this study was to investigate deep-seated soft tissue sarcoma (STS) occurring in the adductor compartment of the thigh that underwent wide resection and to clarify the high-risk group for wound complications.Patients and methodsFrom 2000 to 2017, we reviewed 104 cases of deep-seated STS occurring in the adductor compartment of the thigh that were treated at four specialized facilities with expertise in sarcoma treatment.ResultsWound complications occurred in 40 cases (38.5%), of which 23 cases (22.1%) were cases with major wound complications (MWC). In univariate analysis, BMI (P &lt; 0.01), maximum tumor diameter (P &lt; 0.01), operation time (P &lt; 0.01), amount of intraoperative bleeding (P &lt; 0.01), and intraoperative intervention to the femoral artery and vein (P &lt; 0.01) were significantly associated with wound complications. In multivariate analysis, the associated parameters were BMI (P &lt; 0.01), maximum tumor diameter (P = 0.02), and intraoperative intervention to the femoral artery and vein (P = 0.01). When limited to cases with MWC, univariate analysis showed that maximum tumor diameter (P &lt; 0.01), diabetes mellitus (P = 0.03), operation time (P &lt; 0.01), amount of intraoperative bleeding (P &lt; 0.01), and intraoperative intervention to the femoral artery and vein (P = 0.02) were significantly associated parameters. In multivariate analysis, maximum tumor diameter (P = 0.02) and amount of intraoperative bleeding (P = 0.04) were associated parameters.ConclusionsFor patients with risk factors for wound complications, control of bleeding are crucial when resecting deep-seated STS in the adductor compartment of the thigh. In cases with large tumors, surgeons should be especially cautious of cases requiring interventions that surround the femoral artery and vein in order to attain an appropriate surgical margin.
Laparoscopic anatomical segment 3 segmentectomy for hepatocellular carcinoma accompanied by hypoplasia of the right hepatic lobe
Authors: Yamane Hisoka , Yoshida Sachiko , Yoshida Toshihiko , Nishi Masayasu , Yamagishi Takashi , Goto Hironobu , Otsubo Dai , Furutani Akinobu , Matsumoto Taku , Fujino Yasuhiro , Tominaga Masahiro 
Published: June 2019

Abstract
We report a case of laparoscopic anatomical segment 3 segmentectomy for hepatocellular carcinoma (HCC) accompanied by hypoplasia of the right hepatic lobe. An 80-year-old man was admitted with a suspicion of HCC diagnosed by computed tomography during follow-up for thyroid cancer. Dynamic computed tomography showed 40-mm HCC in segment 3 and hypoplasia of the right hepatic lobe with the Chilaiditi sign. We performed laparoscopic anatomical segment 3 segmentectomy. There were no postoperative complications, and the patient was discharged 6 days postoperatively. This procedure can be performed safely and is technically feasible, but special attention should be paid to anatomical alterations to avoid fatal surgical complications.
Sulfated plant peptide hormones
Authors: Kaufmann Christine , Sauter Margret 
Published: June 2019

Abstract
Sulfated peptides are plant hormones that are active at nanomolar concentrations. The sulfation at one or more tyrosine residues is catalysed by tyrosylprotein sulfotransferase (TPST), which is encoded by a single-copy gene. The sulfate group is provided by the co-substrate 3´-phosphoadenosine 5´-phosphosulfate (PAPS), which links synthesis of sulfated signaling peptides to sulfur metabolism. The precursor proteins share a conserved DY-motif that is implicated in specifying tyrosine sulfation. Several sulfated peptides undergo additional modification such as hydroxylation of proline and glycosylation of hydroxyproline. The modifications render the secreted signaling molecules active and stable. Several sulfated signaling peptides have been shown to be perceived by leucine-rich repeat receptor-like kinases (LRR-RLKs) but have signaling pathways that, for the most part, are yet to be elucidated. Sulfated peptide hormones regulate growth and a wide variety of developmental processes, and intricately modulate immunity to pathogens. While basic research on sulfated peptides has made steady progress, their potential in agricultural and pharmaceutical applications has yet to be explored.
Genotypic variation and nitrogen stress effects on root anatomy in maize are node specific
Authors: Yang Jennifer T , Schneider Hannah M , Brown Kathleen M , Lynch Jonathan P 
Published: June 2019

Abstract
Root phenotypes that improve nitrogen acquisition are avenues for crop improvement. Root anatomy affects resource capture, metabolic cost, hydraulic conductance, anchorage, and soil penetration. Cereal root phenotyping has centered on primary, seminal, and early nodal roots, yet critical nitrogen uptake occurs when the nodal root system is well developed. This study examined root anatomy across nodes in field-grown maize (Zea mays L.) hybrid and inbred lines under high and low nitrogen regimes. Genotypes with high nitrogen use efficiency (NUE) had larger root diameter and less cortical aerenchyma across nodes under stress than genotypes with lower NUE. Anatomical phenes displayed slightly hyperallometric relationships to shoot biomass. Anatomical plasticity varied across genotypes; most genotypes decreased root diameter under stress when averaged across nodes. Cortex, stele, total metaxylem vessel areas, and cortical cell file and metaxylem vessel numbers scaled strongly with root diameter across nodes. Within nodes, metaxylem vessel size and cortical cell size were correlated, and root anatomical phenotypes in the first and second nodes were not representative of subsequent nodes. Node, genotype, and nitrogen treatment affect root anatomy. Understanding nodal variation in root phenes will enable the development of plants that are adapted to low nitrogen conditions.
Untimely Developments: Genre Drag and Gish Jen’s Mona in the Promised Land
Authors: Lee Wendy Allison 
Published: June 2019

Abstract
Through a reinterpretation of Gish Jen’s novel Mona in the Promised Land (1996), this essay makes the case for the queer possibilities immanent to the most conservative “family values” Asian American genre—the bildungsroman organized around intergenerational conflict. In a decade obsessed with the question of what shifting racial and ethnic demographics meant for the national future, Mona’s content tempted 1990s readers to interpret its vision of the 1960s as a timely meditation on the present. Read in such a way, the novel resolves anxieties about demographic change by reproducing the “timeless” values of family and nation. However, Mona’s form tells a different story. Rather than using the historical past as a mere backdrop to tell a timely story about national progress, Mona is reflexive in its preoccupation with its relationship to the past. Jen’s novel shows us what a coming-of-age story looks like when it does not assimilate its subject into national time. Instead, Mona draws “untimely comparisons” between past US imperial formations that are the present’s condition of possibility. I examine how the novel disrupts the bildungroman’s formal and ideological relationship to national futurity by evoking the past as a drag on progress and the novel as enacting a formal corollary to queer drag performance. I read Mona as a novel of untimely development that reinvents the coming-of-age narrative so that the Asian American subject becomes not a figure that exemplifies a certain future subject or nation but instead one that generatively obstructs national fantasies of progress.
Exogenous RNAi mechanisms contribute to transcriptome adaptation by phased siRNA clusters in Paramecium
Authors: Karunanithi Sivarajan , Oruganti Vidya , Marker Simone , Rodriguez-Viana Angela M , Drews Franziska , Pirritano Marcello , Nordström Karl , Simon Martin , Schulz Marcel H 
Published: June 2019

AbstractExtensive research has characterized distinct exogenous RNAi pathways interfering in gene expression during vegetative growth of the unicellular model ciliate Paramecium. However, role of RNAi in endogenous transcriptome regulation, and environmental adaptation is unknown. Here, we describe the first genome-wide profiling of endogenous sRNAs in context of different transcriptomic states (serotypes). We developed a pipeline to identify, and characterize 2602 siRNA producing clusters (SRCs). Our data show no evidence that SRCs produce miRNAs, and in contrast to other species, no preference for strand specificity of siRNAs. Interestingly, most SRCs overlap coding genes and a separate group show siRNA phasing along the entire open reading frame, suggesting that the mRNA transcript serves as a source for siRNAs. Integrative analysis of siRNA abundance and gene expression levels revealed surprisingly that mRNA and siRNA show negative as well as positive associations. Two RNA-dependent RNA Polymerase mutants, RDR1 and RDR2, show a drastic loss of siRNAs especially in phased SRCs accompanied with increased mRNA levels. Importantly, most SRCs depend on both RDRs, reminiscent to primary siRNAs in the RNAi against exogenous RNA, indicating mechanistic overlaps between exogenous and endogenous RNAi contributing to flexible transcriptome adaptation.
Cholera outbreak during a scientific conference at a Nairobi hotel, Kenya 2017
Authors: Mwenda Valerian , Niyomwungere Alexis , Oyugi Elvis , Githuku Jane , Obonyo Mark , Gura Zeinab 
Published: June 2019

Abstract

Background
Cholera globally affects 1.3–4.0 million people and causes 21 000–143 000 deaths annually. In June 2017, a cluster of diarrhoeal illness occurred among participants of an international scientific conference at a hotel in Nairobi, Kenya. Culture confirmed Vibrio cholerae, serotype Ogawa. We investigated to assess magnitude, identify likely exposures and suggest control measures.

Methods
We carried out a retrospective cohort study utilizing a structured questionnaire administered by telephone, email and internet-based survey. We calculated food-specific attack rates, risk ratios and in a nested-case control analysis, performed logistic regression to identify exposures independently associated with the outbreak.

Results
We interviewed 249 out of 456 conference attendees (response rate=54.6%). Mean age of respondents was 37.8 years, ±8.3 years, 131 (52.6%) were male. Of all the respondents, 137 (55.0%) were cases. Median incubation time was 35 (11–59) hours. Eating chicken (adjusted OR 2.49, 95% CI, 1.22–5.06) and having eaten lunch on Tuesday (adjusted OR 2.34, 95% CI 1.09–5.05) were independently associated with illness; drinking soda was protective (adjusted OR 0.17, 95% CI 0.07–0.42).

Conclusion
Point source outbreak, associated with chicken eaten at lunch on Tuesday 20th June 2017 occurred. We recommend better collaboration between the food and health sectors in food-borne outbreak investigations.
Towards therapeutic drug monitoring of TNF inhibitors for children with juvenile idiopathic arthritis: a scoping review
Authors: Verstegen Ruud H J , McMillan Rhona , Feldman Brian M , Ito Shinya , Laxer Ronald M 
Published: June 2019

Abstract

Objectives
Before a clinician decides whether treatment with TNF inhibition in children with JIA has failed, one should ensure adequate systemic exposure has been achieved. Therapeutic drug monitoring might allow for improved treatment outcome with lower treatment-associated costs. However, this requires understanding of the pharmacokinetic (PK) characteristics, and the pharmacokinetic/pharmacodynamic (PK/PD) relationship for children with JIA. We performed a scoping review to summarize the available literature and identify areas for future research.

Methods
A systematic search was conducted of the Medline, EMBASE, Web of Science and Cochrane databases as well as the clinicaltrials.gov registry. In total, 3959 records were screened and 130 publications were selected for full text assessment.

Results
Twenty publications were included and divided into three categories: PK (n = 9), PK/PD (n = 3) and anti-drug antibodies (n = 13). Industry involvement was significant in 14 publications. Although data are limited, systemic exposure to TNF inhibitors is generally lower in younger children but meta-analysis is not possible. The PK/PD relationship has had limited study but there is partial evidence for infliximab. Anti-drug antibodies are common, and are related to impaired clinical outcome with adalimumab and infliximab therapy.

Conclusion
The current knowledge about the PK and PK/PD of TNF inhibitors in the treatment of children with JIA is limited, which prevents the introduction of TDM. Re-analysis of available data from previous trials, incorporation of pharmacologic assessments into existing biorepository studies as well as new prospective PK and PK/PD trials are required to obtain this knowledge.
Trust law in Chinese courts: judicial decisions as data (2001–2017)
Authors: Qu Jian 
Published: June 2019

Abstract
Trust law was transplanted into China nearly two decades ago, but how has it been applied by the Chinese courts? The answer may be found in relevant judgments. This study collected accessible Chinese court decisions related to trusts, and, relying on this, the citation practice of Trust Law in courts can be analyzed chronologically, and intensively applied articles can be singled out. Thus, this study intends to use these judicial documents, as data and as individual cases, to examine the role that the Chinese Trust Law has played in the judicial field since its enactment in 2001.