bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Parents School Program at Baturraden Natural School
Authors: Alimuddin Johar , Safar Mira Purnamasari 
Published: June 2019

<p class="Abstract">The aims of this research were to reveal: (a) The concept and aim of The Parent’s School that is implemented in Baturraden Nature School (b) Activities that are done by parents when they are involved in children education. (c) Challenges from the implementation of the parent’s school program. This research applied case study qualitative approach because it wanted to reveal what is behind the parent’s school program that is implemented in Baturraden Nature School. This research also revealed any activity that involved the parents in the educational practice in Baturraden nature school. Moreover, it also revealed challenges as well as the things that attribute the implementation of the parent’s school activity in Baturraden nature school. The research data was gained from interview, observation, and document analysis. The parent’s school is a parent’s involvement program in children education that is considered as parent’s education needs model. The form of the parent’s school was not only conference, but it was varied through a variety of activities such as outbound or games. The parent’s school activity that has been joined by the students’ parents were closed with reflection and question and answer with psychologist or expert. Parents were also involved in the children’s assignment or project at home. This parent’s school activity was high almost ever students’ parents in the Baturraden nature school attended. This parent’ school program was very helpful in monitoring the students’ development as well as able to increase the children’s potential.</p>
Special Issue on Education Based on Practical Exercise on Sensing and Control
Authors: Fujisawa Shoichiro , Kawada Kazuo , Ohnishi Yoshihiro 
Published: June 2019

Control engineering and sensing engineering improve productivity and save resources and energy in industry, and they are also deeply related to the solving greater societal, economic, and environmental problems. Control engineering and sensing engineering have become dynamic forces that enrich various phases of life through interdisciplinary or cross-sectional study. Furthermore, in recent years, due to the development of information technology, as symbolized by terms such as “big data” or “AI,” “sensing and control at a higher level” has become possible, premised by big data processing that is faster by orders of magnitude than conventional data processing. All this has increased the importance of control engineering and sensing engineering.
In response to the development of the fields of control engineering and sensing engineering associated with the advance of the “information society,” education in these fields has also needed to be enhanced. On the national scale, the Ministry of Education, Culture, Sports, Science and Technology will introduce Japanese elementary school computational thinking education into elementary school in fiscal year 2020, and the new Courses of Study for High School Information Education in fiscal year 2022. At the same time, individual companies, educational institutions, etc. have also been experimenting with various forms of education in control engineering and sensing engineering. During these changing times, the most advanced studies related to the development of instruction and evaluation methods for educational materials on control engineering, sensing engineering, and control technology have been collected, and the present special issue was planned.
This special issue is a collection of practical papers related to measurement and control education, including one paper on Model-Based Development education in a company and eight papers on education in an educational institution. These eight papers include two on education using a robot contest in a university, one on introducing measurement and control engineering education in a national institute of technology college, three on introducing it in a junior high school, and two on introducing it in an elementary school.
We hope that this special issue serves to support the readers’ future efforts in control engineering and sensing engineering education, and we thank the authors and reviewers of the papers.
Effects of Cerebrospinal Fluid AMPA Receptor Levels on the Clinical Efficacy of Lamotrigine in the Treatment of Epilepsy
Authors: Tan Qingjing , Liu Yonghui , Wei Tianlu , Yang Junwei , Wang Tianbao , Lin Haohai 
Published: June 2019

Abstract
Abstract
Background ：Epilepsy is one of great health burden in the world, deeply effects epilepsy population’s mental and physical health,some can totally recover through effective treatment, while some others are difficult to recover even have life risks when seizure attacks. In that case,we explore the effects of cerebrospinal fluid AMPA receptor levels on the clinical efficacy of lamotrigine in the treatment of epilepsy.We believe our work might have an implication in the treatment of epilepsy.
Methods ：The 70 cases of epilepsy in our hospital were diagnosed and selected in this study from December 2016 to October 2018; The AMPA receptor content of patients in cerebrospinal fluid were determined by enzyme linked immunosorbent assay; The patients were paired into high AMPA group (n=34) and low AMPA group (n=36) according to the median value at 4.08 ng/ml. Clinical efficacy and the incidence of adverse reactions were compared between two groups.
Results ：Before treatment, there was no significant difference in seizure frequency between the two groups (P>0.05); After treatment for 6 months and 12 months, the seizure frequencies of the two groups were gradually reduced (P<0.05); Moreover, the number of seizures in the low AMPA group were significantly less than those in the high AMPA group (P<0.05); The response rate of high AMPA group was 79.41%, which was significantly lower than that in the low group AMPA (χ2=6.055, P=0.048); The improvement on the efficiency of electroencephalogram in the high AMPA group was 67.65%, which was significantly lower than that in the low AMPA group (χ2=4.686, P=0.030); However, there was no significant on the incidence of adverse reactions between two groups (χ2=0.202, P=0.653).
Conclusions： AMPA receptor plays an important role in the development of epilepsy and the low AMPA receptor level patients were more efficacies to the treatment of lamotrigine.
Keywords：Lamotrigine; Epilepsy; AMPA receptor.
GhFAD2-3 is Required for Anther Development in Gossypium hirsutum
Authors: Liu Feng , Ma Lihong , Wang Youwu , Li Yanjun , Zhang Xinyu , Xue Fei , Nie Xinhui , Zhu Qianhao , Sun Jie 
Published: June 2019

Abstract
Background

In higher plants, FAD2 gene encodes the microsomal oleate Δ12-desaturase, one of the key enzymes essential for biosynthesis of polyunsaturated lipids that serve many important functions in plant development and stress responses. FAD2 catalyzes the first step, towards biosynthesis of polyunsaturated fatty acids (PUFAs) found in cell membrane and wall, and it is thus of great importance to investigate the regulatory role of FAD2 in anther development.

Results

We reported the molecular characterization of the cotton (Gossypium hirsutum) GhFAD2 gene family and the essential role of GhFAD2-3 in cotton anther development. G. hirsutum contains four pairs of homoeologous FAD2 genes (GhFAD2-1 to GhFAD2-4). GhFAD2-3 is ubiquitously and relatively highly expressed in all tissues analyzed, particularly in anthers. Specific inhibition of GhFAD2-3 using the RNA interference approach resulted in male sterility due to impaired anther development at the stages from meiosis to maturation. The cellular phenotypic abnormality observed at the meiosis stage of the GhFAD2-3 silenced plant (fad2-3) coincides with the significant reduction of C18:2 in anthers at the same stage. Compared with that of wild type (WT), the content of C18:1 was 41.48%, accordingly increasing by 5 fold in fad2-3 anther at pollen maturation stage. Moreover, the ration of monounsaturated and polyunsaturated fatty acid reached to 5.43 in fad2-3 anther, far beyond WT that of which was only 0.39. Based on compositional analysis of anthers cuticle and transcriptome data, we demonstrated it was unfavorable to the development of anther by regulating GhFAD2-3 expression level to increase the oleic acid content.

Conclusions

Our work demonstrated the importance of C18:2 and/or C18:3 in development of pollen exine and anther cuticle in cotton, and provided clue for further investigation of the physiological significance of the fatty acid composition in plant growth and development.
Temporary increase in tidal volume to improve the reliability of dynamic preload indices during robot-assisted laparoscopic surgery in the Trendelenburg position with lung-protective ventilation
Authors: Jun Joo-Hyun , Chung Rack Kyung , Baik Hee Jung , Chung Mi Hwa , Hyeon Joon-Sang , Lee Young-Goo , Park Sung-Ho 
Published: June 2019

Abstract
Background: Pulse pressure variation (PPV) and stroke volume variation (SVV) induced by mechanical ventilation are widely used as predictors of fluid responsiveness. However, the reliability of these dynamic preload indices is controversial under pneumoperitoneum. In addition, the usefulness of these indices is being called into question with the increasing adoption of lung-protective ventilation using low tidal volume (VT) in surgical patients. We investigated whether increasing tidal volume (VT) from 6 to 8 ml/kg can improve the predictive power of PPV and SVV during pneurmoperitoneum. Methods: We performed a prospective observational study in patients undergoing robot-assisted laparoscopic surgery in the Trendelenburg position under lung-protective ventilation. PPV, SVV, and the stroke volume index (SVI) were measured at a VT of 6 mL/kg and 3 minutes after increasing the VT to 8 mL/kg. The VT was reduced to 6 mL/kg, and measurements were performed before and 5 minutes after volume expansion (infusing 6% hydroxyethyl starch 6 ml/kg over 10 minutes). Fluid responsiveness was defined as ≥ 15% increase in the SVI. Results: Twenty-four of the 38 patients enrolled in the study were responders. In the receiver operating characteristic curve analysis, the augmented PPV and SVV associated with a temporary increase in VT from 6 to 8 ml/kg improved the predictability of fluid responsiveness, with area under the curve (AUC) values of 0.85 (95% confidence interval (CI), 0.70–0.95, P < 0.0001) and 0.77 (95% CI 0.61–0.89, P = 0.0003), compared to PPV and SVV values (as measured by VT) of 6 ml/kg. The absolute change in PPV and SVV values obtained by transiently increasing VT also predicted fluid responsiveness, with AUC values of 0.95 (95% CI 0.83–0.99, P < 0.0001) and 0.76 (95% CI 0.60–0.89, P = 0.0006). Conclusions: Augmented PPV and SVV values, and absolute changes therein obtained by increasing VT from 6 to 8 ml/kg, predicted fluid responsiveness with high sensitivity and specificity in our surgical population.
Myasthenic crisis treated in a Chinese neurological intensive care unit: clinical features, mortality, outcomes, and predictors of survival
Authors: Liu Fan , Wang Qiong , Chen Xueping 
Published: June 2019

Abstract
Background: Myasthenic crisis (MC) often requires admission to an intensive care unit (ICU).
Methods: We retrospectively investigated 113 consecutive patients with first MC admitted to the neurological ICU. Patients’ demographic, clinical and other characteristics were examined, as well as therapeutic interventions, mortality and functional outcome.
Results: MC patients at first onset admitted to neurological ICU had a mortality rate of 18.6%. PCO2 level before intubation and score on Myasthenia Gravis–Activities of Daily Living (MG-ADL) scale at MC onset correlated with duration of ventilation and length of ICU stay. Compared with patients with good functional outcome, patients with intermediate or poor functional outcome were older at first MC onset, had lower pH and PO2, and had higher PCO2 before intubation. Multivariate logistic analysis identified pre-intubation PCO2 level as an independent predictor of survival. Cox regression showed that age at first MC onset requiring ICU management was the factor which significantly influenced the mortality.
Conclusions: Our results suggest that PCO2 before intubation and MG-ADL score at MC onset may be useful indicators of more severe disease likely to require extensive respiratory support and ICU management. Higher pre-intubation PCO2 indicates chronic respiratory acidosis that can increase risk of severe disability and death, especially in patients with older age at first MC onset.
Effects of the Chinese herbal formula San-Huang Gu-Ben Zhi-Ke treatment on Stable Chronic Obstructive Pulmonary disease:study protocol of a randomized, double-blind, placebo-controlled trial
Authors: Zu Yaqi , Li Demin , Lei Xiang , Zhang Hongchun 
Published: June 2019

Abstract
Background

Due to the large number of patients, high mortality rate as well as high social costs and economic burden, Chronic obstructive pulmonary disease(COPD)has become one of the most important health problem around the world, which has attracted people's attention. Currently, Chinese herbs have been widely used as alternative medicine(CAM)for COPD patients. Chinese herbal formula San-Huang Gu-Ben Zhi-Ke(SHGBZK)have shown good clinical efficacy in COPD in preclinical studies. Animal experiments have enhanced that it has mucosal immune barrier function, it can maintain airway wall integrity, reduce inflammatory cell infiltration, promote inflammatory damage repair, and relieve airway narrow conditions.

Methods/design

The study is a randomized, double-blind, placebo-controlled trial. A total of 100 COPD patients at stable stage that diagnosed with syndrome of deficiency of lung qi and spleen qi will be recruited and randomly assigned to one of the two treatments group, with 50 in each group. One hundred COPD patients were randomly assigned to two treatment groups（SHGBZK treatment, N=50; placebo treatment, N=50).The two groups will receive basic treatment for COPD according to the 2017 GOLD Guidelines for Chronic Obstructive Pulmonary Disease. Patients will stick to the treatment they used to take as much as possible, and will be given present general treatment when AECOPD occurs during the study. Both groups will receive an 24-weeks intervention, and patient status was assessed at 24 weeks later and 28 weeks after treatment. After the 24-weeks treatment, another 28 weeks will be followed up. The outcome measures including the frequency and duration of acute exacerbation, lung function, TCM symptom score, exercise capacity, and quality of life will be assessed.

Discussion

It is hypothesized that SHGBZK will have beneficial effects in reducing the frequency and duration of acute exacerbation, improving exercise capacity function of COPD patients at stable stage that diagnosed with syndrome of deficiency of lung qi and spleen qi. This study may establish a new method for COPD patients, and thus differentiation from other drugs used for similar clinical indications in clinical use.
Depression in the nursing home: a cluster-randomized stepped-wedge study to probe the effectiveness of a novel case management approach to improve treatment (the DAVOS project)
Authors: Tesky Valentina Antonia , Schall Arthur , Schulze Ulrike , Stangier Ulrich , Oswald Frank , Knopf Monika , König Jochem , Blettner Maria , Arens Elisabeth , Pantel Johannes 
Published: June 2019

Abstract
Background

Depression is the second most common psychiatric illness in old people. Up to 30% of nursing home residents suffer from minor or major depression. Although depressive disorders in old age can be improved and even cured with adequate therapy, they often go unnoticed in nursing home residents and remain untreated. This highlights a striking deficit in health care and might results not only in lower quality of life among those concerned but also in poor physical functioning, premature mortality and increased hospitalization rates.

Methods

The aim of the interdisciplinary research project DAVOS is to implement an innovative and stepped structural case management program to improve depression treatment in nursing home residents by a modularized intervention and to assess it in terms of its effectiveness. Intervention modules are in line with recommendations given by the German national treatment guidelines for depression (S3 guidelines). Ten nursing homes in Frankfurt, Germany, will participate in the project which aims to recruit a study population of N = 380. The recruitment will continue throughout the trial (open cohort). Persons (>60 years) that live in a nursing home, have no medical diagnosis of dementia and can provide their informed consent to participate are eligible for inclusion in the study. Residents with a clinical diagnosis of dementia, alcohol or substance related disorders or other serious psychiatric illnesses will be excluded. DAVOS is a controlled cluster-randomized study that employs a stepped-wedge design.

Discussion

Our main hypothesis is that the implementation of the intervention will lead to a decline in the prevalence of depression and a reduction in depression symptoms among the home residents. In addition, we expect the intervention to have a positive impact on secondary outcomes such as level of functioning, quality of life and social participation. The project’s results can make an important contribution towards improving the health care of nursing home residents suffering from late-life depression.
Thermostability of Lipase A and Dynamic Communication Based on Residue Interaction Network
Authors: Xia Qian , Ding Yanrui 
Published: June 2019

Objective:
Dynamic communication caused by mutation affects protein stability. The
main objective of this study is to explore how mutations affect communication and to provide
further insight into the relationship between heat resistance and signal propagation of Bacillus
subtilis lipase (Lip A).

Methods:
The relationship between dynamic communication and Lip A thermostability is studied
by long-time MD simulation and residue interaction network. The Dijkstra algorithm is used to get
the shortest path of each residue pair. Subsequently, time-series frequent paths and spatio-temporal
frequent paths are mined through an Apriori-like algorithm.

Results:
Time-series frequent paths show that the communication between residue pairs, both in
wild-type lipase (WTL) and mutant 6B, becomes chaotic with an increase in temperature; however,
more residues in 6B can maintain stable communication at high temperature, which may be
associated with the structural rigidity. Furthermore, spatio-temporal frequent paths reflect the
interactions among secondary structures. For WTL at 300K, β7, αC, αB, the longest loop, αA and αF
contact frequently. The 310-helix between β3 and αA is penetrated by spatio-temporal frequent paths.
At 400K, only αC can be frequently transmitted. For 6B, when at 300K, αA and αF are in more tight
contact by spatio-temporal frequent paths though I157M and N166Y. Moreover, the rigidity of the
active site His156 and the C-terminal of Lip A are increased, as reflected by the spatio-temporal
frequent paths. At 400K, αA and αF, 310-helix between β3 and αA, the longest loop, and the loop
where the active site Asp133 is located can still maintain stable communication.

Conclusion:
From the perspective of residue dynamic communication, it is obviously found that
mutations cause changes in interactions between secondary structures and enhance the rigidity of
the structure, contributing to the thermal stability and functional activity of 6B.
pH-Dependent Thermal Stability of Vibrio cholerae L-asparaginase
Authors: Radha Remya , Gummadi Sathyanarayana N. 
Published: June 2019

Background:pH is one of the decisive macromolecular properties of proteins that significantly affects enzyme structure, stability and reaction rate. Change in pH may protonate or deprotonate the side group of aminoacid residues in the protein, thereby resulting in changes in chemical and structural features. Hence studies on the kinetics of enzyme deactivation by pH are important for assessing the bio-functionality of industrial enzymes. L-asparaginase is one such important enzyme that has potent applications in cancer therapy and food industry.Objective:The objective of the study is to understand and analyze the influence of pH on deactivation and stability of Vibrio cholerae L-asparaginase.Methods:Kinetic studies were conducted to analyze the effect of pH on stability and deactivation of Vibrio cholerae L-asparaginase. Circular Dichroism (CD) and Differential Scanning Calorimetry (DSC) studies have been carried out to understand the pH-dependent conformational changes in the secondary structure of V. cholerae L-asparaginase.Results:The enzyme was found to be least stable at extreme acidic conditions (pH< 4.5) and exhibited a gradual increase in melting temperature from 40 to 81 °C within pH range of 4.0 to 7.0. Thermodynamic properties of protein were estimated and at pH 7.0 the protein exhibited ΔG37of 26.31 kcal mole-1, ΔH of 204.27 kcal mole-1 and ΔS of 574.06 cal mole-1 K-1.Conclusion:The stability and thermodynamic analysis revealed that V. cholerae L-asparaginase was highly stable over a wide range of pH, with the highest stability in the pH range of 5.0–7.0.
Metal-Organic Framework (MOF)-Based Drug Delivery
Authors: Cao Jian , Li Xuejiao , Tian Hongqi 
Published: June 2019

Background:
Developing a controllable drug delivery system is imperative and important
to reduce side effects and enhance the therapeutic efficacy of drugs. Metal-organic frameworks
(MOFs) an emerging class of hybrid porous materials built from metal ions or clusters bridged by
organic linkers have attracted increasing attention in the recent years owing to the unique physical
structures possessed, and the potential for vast applications. The superior properties of MOFs, such
as well-defined pore aperture, tailorable composition and structure, tunable size, versatile functionality,
high agent loading, and improved biocompatibility, have made them promising candidates as
drug delivery hosts. MOFs for drug delivery is of great interest and many very promising results
have been found, indicating that these porous solids exhibit several advantages over existing systems.

Objective:
This review highlights the latest advances in the synthesis, functionalization, and applications
of MOFs in drug delivery, and has classified them using drug loading strategies. Finally, challenges
and future perspectives in this research area are also outlined.
QSAR Models for Predicting Aquatic Toxicity of Esters Using Genetic Algorithm-Multiple Linear Regression Methods
Authors: Rajabi Mehdi , Shafiei Fatemeh 
Published: June 2019

Aim and Objective:
Esters are of great importance in industry, medicine, and space
studies. Therefore, studying the toxicity of esters is very important. In this research, a Quantitative
Structure–Activity Relationship (QSAR) model was proposed for the prediction of aquatic toxicity
(log 1/IGC50) of aliphatic esters towards Tetrahymena pyriformis using molecular descriptors.

Materials and Methods:
A data set of 48 aliphatic esters was separated into a training set of 34
compounds and a test set of 14 compounds. A large number of molecular descriptors were
calculated with Dragon software. The Genetic Algorithm (GA) and Multiple Linear Regression
(MLR) methods were used to select the suitable descriptors and to generate the correlation models
that relate the chemical structural features to the biological activities.

Results:
The predictive powers of the MLR models are discussed by using Leave-One-Out (LOO)
cross-validation and external test set. The best QSAR model is obtained with R2 value of 0.899, Q2
LOO =0.928, F=137.73, RMSE=0.263.

Conclusion:
The predictive ability of the GA-MLR model with two selected molecular descriptors
is satisfactory and it can be used for designing similar group and predicting of toxicity (log
1/IGC50) of ester derivatives.