bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relation of High‐Density Lipoprotein Charge Heterogeneity, Cholesterol Efflux Capacity, and the Expression of High‐Density Lipoprotein‐Related Genes in Mononuclear Cells to the HDL‐Cholesterol Level
Authors: Dergunov Alexander D. , Litvinov Dmitry Y. , Bazaeva Ekaterina V. , Dmitrieva Veronika G. , Nosova Elena V. , Rozhkova Alexandra V. , Dergunova Liudmila V. 
Published: November 2018

AbstractThe heterogeneity and content of human plasma high‐density lipoprotein (HDL) related to their atheroprotective properties determined by various molecular and cellular mechanisms still remain to be completely clarified. For 29 atherosclerosis‐free male subjects, we studied the relationship of plasma lipid levels and the content of apolipoprotein A‐I (apoA‐I)‐containing HDL with preβ‐electrophoretic mobility, the efficiency of BODIPY‐cholesterol efflux from RAW 264.7 macrophages to apolipoprotein B (apoB)‐deficient plasma, and the expression level of 22 genes related to HDL metabolism in mononuclear cells. A significant decrease in the absolute content of apoA‐I in preβ‐HDL was found in subjects with hypoalphalipoproteinemia compared with the subjects with hyperalphalipoproteinemia. The preβ‐to‐α‐ratio of the apoA‐I content was constant within the HDL‐cholesterol (HDL‐C) range 0.59 to 2.24 mM. However, this ratio was significantly increased with an increase in the plasma triacylglycerol (TAG) content from 0.59 to 3.42 mM. A correlation of the level of preβ‐HDL with the basal and ABCA1‐mediated efflux of cholesterol is shown. The transcript levels for six HDL‐metabolizing genes (LDLR, LCAT, ABCA1, SCARB1, ZDHHC8, and BMP1) were decreased, while the transcript level of APOA1 gene was increased in mononuclear cells of subjects with hyperalphalipoproteinemia as compared with subjects with hypoalphalipoproteinemia. A reduction of the intracellular cholesterol level and inhibition of the expression of cholesterol transporters by nascent HDL in mononuclear cells from subjects with hyperalphalipoproteinemia are suggested. Hyperalphalipoproteinemia can be a driving force of the decreased flux of cholesteryl ester to the liver and the increased TAG hydrolysis. The atheroprotective effect of preβ‐HDL in hypertriglyceridemia is proposed.
Construction of Bovine Serum Albumin/AIE‐Based Quaternary Complexes for Efficient Gene Transfection
Authors: Liu De‐E , An Jinxia , Pang Chengcai , Yan Xiangjie , Li Wei , Ma Jianbiao , Gao Hui 
Published: November 2018

AbstractHigh transfection efficiency and superior cell imaging are required for cationic polymers‐based gene delivery system to afford high therapeutic effect but its high toxicity and unstable cell imaging are easily ignored. In this study, cationic amino poly(glycerol methacrylate) derivative (PGMA‐EDA) is used to incorporate bovine serum albumin (BSA) and aggregation‐induced emission (AIE) molecular (tetraphenylethylene derivatives, TPE) as an efficient carrier for gene transfection and intracellular imaging. The obtained polymer/pDNA‐TPE/BSA (PDTB) quaternary nanoparticles (NPs) not only exhibit efficient gene transfection but also show excellent biocompatibility. After inclusion of TPE/BSA (TB) NPs, BSA promoted dissociation of the complexes upon being protonated and the lipophilic TPE‐reduced endosomal membrane stability, which enhanced endosomal escape of pDNA payload, finally resulting in an excellent gene transfection. On the other hand, less positive surface charge of PDTB NPs than that of the binary PD complexes, as well as the addition of biocompatible BSA, both factors contribute to the improved cell viability. Moreover, the AIE feature of TPE compared to aggregation‐caused quenching character of conventional fluorophores enables the complex with stably tracking the delivery of pDNA into cancer cells. Therefore, the newly developed PDTB complexes may be a promising candidate vector for traceable, safe, and effective gene delivery.
Progressively disrupted somatodendritic morphology in dopamine neurons in a mouse Parkinson's model
Authors: Lynch William B. , Tschumi Christopher W. , Sharpe Amanda L. , Branch Sarah Y. , Chen Cang , Ge Guo , Li Senlin , Beckstead Michael J. 
Published: November 2018

ABSTRACTBackgroundParkinson's disease is characterized by the progressive loss of dopamine neurons in the substantia nigra, leading to severe motor deficits. Although the disease likely begins to develop years before observable motor symptoms, the specific morphological and functional alterations involved are poorly understood.ObjectivesMitoPark mice lack the gene coding for mitochondrial transcription factor A specifically in dopamine neurons, which over time produces a progressive decline of neuronal function and related behavior that phenotypically mirrors human parkinsonism. Our previous work identified a progressive decrease in cell capacitance in dopamine neurons from MitoPark mice, possibly suggesting reduced membrane surface area. We therefore sought to identify and quantify somatodendritic parameters in this model across age.MethodsWe used whole‐cell patch clamp and fluorescent labeling to quantify somatodendritic morphology of single, neurobiotin‐filled dopamine neurons in acutely isolated brain slices from MitoPark mice.ResultsWe found that MitoPark mice exhibit an adult‐onset, age‐dependent reduction of neuritic branching and soma size in dopamine neurons. This decline proceeds similarly in MitoPark mice of both sexes, but does not begin until after the age that early decrements in ion channel physiology and behavior have previously been observed.ConclusionsA progressive and severe decline in somatodendritic morphology occurs prior to cell death, but is not responsible for the subtle decrements observable in the earliest stages of neurodegeneration. This work could help identify the ideal time window for specific treatments to halt disease progression and avert debilitating motor deficits in Parkinson's patients. © 2018 International Parkinson and Movement Disorder Society
Robust visualization of middle cerebral artery main trunk by enhanced acceleration‐selective arterial spin labeling (eAccASL) for intracranial MRA
Authors: Akamine Yuta , Obara Makoto , Togao Osamu , Shibukawa Shuhei , Yoneyama Masami , Okuaki Tomoyuki , Van Cauteren Marc 
Published: November 2018

PurposeA new sequence for intracranial MRA is developed, named enhanced acceleration‐selective arterial spin labeling (eAccASL), to improve main artery visualization at middle cerebral artery (MCA). The aim of this study is to assess the visualization improvement using eAccASL, compared with the previously developed AccASL.MethodseAccASL and AccASL were performed in 8 healthy volunteers and images were compared between the 2 sequences. One patient with Moyamoya disease was evaluated by eAccASL and time of flight. For the volunteer images, vessel visualization was assessed by measuring the contrast‐to‐noise ratio between MCA M1 to M4 and white matter and by counting the peripheral arteries. Venous artifact level was assessed by measuring the contrast‐to‐noise ratio between the confluence of the sinuses and white matter and by evaluating cortical vein visualization. For the patient images, qualitative assessment of peripheral and collateral vessel visualization was conducted.ResultsIn the MCA main trunk, higher arterial signal intensity, with reduced flow void, was observed in eAccASL compared with AccASL. Contrast‐to‐noise ratios of M1 to M3 for eAccASL were significantly higher than those of AccASL. There was no significant difference between AccASL and eAccASL for venous artifact.ConclusioneAccASL could produce better MCA main trunk visualization compared with AccASL, while maintaining good venous signal suppression.
A systematic review on reporting outcomes and outcome measures in trials on synthetic mesh procedures for pelvic organ prolapse: Urgent action is needed to improve quality of research
Authors: de Mattos Lourenco Thais R. , Pergialiotis Vasilis , Duffy James M. N. , Durnea Constantin , Elfituri Abdullatif , Haddad Jorge M. , Betschart Cornelia , Falconi Gabriele , Doumouchtsis Stergios K. ,   CHORUS: An International Collaboration for Harmonizing Outcomes, Research and Standards in Urogynaecology and Women's Health
Published: November 2018

The use of synthetic mesh in pelvic organ prolapse surgery is being closely scrutinized because of serious concerns regarding life‐changing complications such as erosion, pain, infection, bleeding, dyspareunia, organ perforation, and urinary problems. Randomized trials and their syntheses in meta‐analysis offer a unique opportunity to assess efficacy and safety. However, outcomes and outcome measures need to be consistently selected, collected, and reported across randomized trials to be effectively combined in systematic reviews.AimsWe evaluated outcome and outcome measure reporting across randomized controlled trials on surgical interventions using synthetic mesh for pelvic organ prolapse.MethodsSystematic review of randomized controlled trials using synthetic mesh for the treatment of pelvic organ prolapse. The selected studies were evaluated using Jadad and MOMENT criteria. Outcomes and outcome measures were systematically identified and categorized.ResultsSeventy‐one randomized trials were included. Twenty‐four different types of mesh were identified. Included trials reported 110 different outcomes and 60 outcome measures. Erosion (40 trials, 78%), pain (29 trials, 56%), bleeding (31 trials, 61%), and dyspareunia (25 trials, 49%) were the most frequently reported outcomes. The longest follow up was 74 months.ConclusionsMost randomized trials evaluating surgical interventions using synthetic mesh for pelvic organ prolapse failed to report on clinically important outcomes and to evaluate efficacy and safety over the medium‐ and long‐term. Developing and implementing a minimum data set, known as a core outcome set, in future vaginal prolapse trials could help address these issues.
MRI evaluation of vulvar squamous‐cell carcinoma in fresh radical local excision specimens for cancer localization and prediction of surgical tumor‐free margins
Authors: Heidkamp Jan , Zusterzeel Petra L.M. , van Engen‐van Grunsven Adriana C.H. , Overduin Christiaan G. , Veltien Andor , Maat Arie , Rovers Maroeska M. , Fütterer Jurgen J. 
Published: November 2018

In the surgical treatment of vulvar squamous‐cell carcinoma (VSCC), tumor‐free margins of 8 mm or more are considered adequate. However, limited perioperative information on the tumor‐free margins other than the surgeon's own estimation is available. The purpose of this study was therefore to investigate the feasibility of ex vivo MRI in localizing VSCC and to assess the surgical tumor‐free margins in fresh radical local excision (RLE) specimens to guide the surgeon during resections. Nine patients with biopsy‐proven VSCC scheduled for RLE were prospectively included. Intact fresh specimens were scanned using a 7 T preclinical MR‐scanner. Whole mount H&E‐stained slides were obtained every 3 mm and correlated with ex vivo MRI. A pathologist annotated VSCC and minimal tumor‐free margins (3 o'clock, 9 o'clock, basal) on the digitalized histological slides. An observer with knowledge of histology (the non‐blinded annotation) and a radiologist blinded to histology (the blinded annotation) separately performed annotation of the same features on ex vivo MRI. Linear correlation and agreement of the ex vivo MRI measurements with histology were assessed. Diagnostic performance for VSCC localization and identification of margins less than 8 mm was expressed as positive and negative predictive values (PPV, NPV). In 153 matched ex vivo MRI slices, the observer correctly identified 79/91 margins as less than 8 mm (PPV 87%) and 110/124 margins as 8 mm or greater (NPV 89%). The radiologist correctly annotated absence of VSCC in 73/81 (NPV 90%) and presence in 65/72 (PPV 90%) slices. Sixty‐four of 90 margins were correctly identified as less than 8 mm (PPV 71%) and 83/102 margins as 8 mm or greater (NPV 81%). Both non‐blinded and blinded annotations were linearly correlated and demonstrated good agreement with histology. Accurate localization of VSCC and measurements of the surgical tumor‐free margins in fresh RLE specimens using ex vivo MRI seems feasible. High diagnostic performance in VSCC localization and identification of margins less than 8 mm suggest ex vivo MRI to be clinically applicable.
Phosphorylated polyacrylonitrile‐based electrospun nanofibers for removal of heavy metal ions from aqueous solution
Authors: Hu Yong , Wu XiaoYong , He Xuling , Xing Diying 
Published: November 2018

The phosphorylated polyacrylonitrile‐based (P‐PAN) nanofibers were prepared by electrospinning technique and used for removal of Cu2+, Ni2+, Cd2+, and Ag+ from aqueous solution. The morphological and structural properties of P‐PAN nanofibers were characterized by scanning electron microscope and Fourie transform infrared spectra. The P‐PAN nanofibers were evaluated for the adsorption capacity at various pH, contact time, and reaction temperature in a batch system. The reusability of P‐PAN nanofibers for the removal of heavy metal ions was also determined. Adsorption isotherms and adsorption kinetics were also used to examine the fundamental adsorption properties. It is found that the P‐PAN nanofibers show high efficiency, and the maximal adsorption capacities of metal ions as calculated from the Langmuir model were 92.1, 68.3, 14.8, and 51.7 mg/g, respectively. The kinetics of the heavy metal ions adsorption were found to follow pseudo‐second‐order rate equation, suggesting chemical adsorption can be regarded as the major factor in the adsorption process. Sorption/desorption results reveal that the obtained P‐PAN nanofibers can remain high removal efficiency after four cycles.
Does school satisfaction predict coping? A short‐term longitudinal examination in early adolescents
Authors: Jiang Xu , Fang Lue , Lyons Michael D. 
Published: November 2018

AbstractWe tested the hypothesis of school satisfaction being an antecedent to social coping behaviors in early adolescents (N = 892) using a two‐wave cross‐lagged panel design. We also explored the possible bidirectional relations between school satisfaction and social coping behaviors. Four types of social coping behaviors in peer conflict contexts included social support seeking, self‐reliance, internalizing behaviors, and externalizing behaviors. The findings showed that school satisfaction significantly predicted three of the four coping behaviors (social support seeking, self‐reliance, and externalizing behaviors) in the expected directions. Among all the social coping behaviors, only social support seeking significantly predicted school satisfaction over time. Taken together, the results suggest that school satisfaction may be an antecedent that predicts most social coping behaviors in early adolescents. Furthermore, the relations between social support seeking coping and school satisfaction appeared to be reciprocal. Implications, especially the importance of monitoring students’ school satisfaction, are discussed.
Relations between sociocultural pressures and weight control behavior among early adolescent boys
Authors: Chu Tsz Lun (Alan) , Martin Scott B. , Petrie Trent A. , Greenleaf Christy 
Published: November 2018

AbstractGuided by the biopsychosocial model, we examined (a) the prevalence of weight control behaviors (WCBs; i.e., trying to stay the same weight, lose weight, gain weight, or do nothing about weight) among early adolescent boys by race/ethnicity and grade level, and (b) how the boys’ perceptions of sociocultural pressures (from parents, peers, and media) to lose weight, gain weight, exercise, and diet predicted their WCBs while accounting for body composition and grade level. Early adolescent boys (
n = 508) in middle schools completed survey measures of WCB and sociocultural pressures and objective assessment of body mass index as body composition. Multinomial logistic regressions revealed that sixth graders were more likely than seventh and eighth graders to be trying to lose weight. Moreover, greater pressure to gain weight and lower pressure to exercise predicted a greater likelihood of trying to gain weight. The significant roles of grade levels and different sociocultural pressures in predicting WCBs suggest that health professionals and school staff should consider these factors when intervening with early adolescent boys.
Consistently inconsistent: An examination of the variability in the identification of emotional disturbance
Authors: Scardamalia Kristin , Bentley‐Edwards Keisha L. , Grasty Kairys 
Published: November 2018

AbstractThe federal definition of emotional disturbance (ED) has been heavily criticized as vaguely defined and poorly operationalized yet there has not been a formal analysis of the reliability of the ED criteria. This study examined the reliability of the federal criteria for a special education designation of ED. A total of 179 school psychologists reviewed a mock special education report, made an eligibility determination, and provided information about their eligibility decision. In all, 56 participants found the student met ED eligibility criteria using 16 different combinations of the five criteria. Krippendorff’s α, a statistic preferred for content analysis, was calculated as a measure of criterion reliability. Results indicated extremely poor reliability (α = 0.2011). These findings demonstrate the importance of redefining the existing criteria.
A chromosome‐scale assembly of the model desiccation tolerant grass Oropetium thomaeum
Authors: VanBuren Robert , Wai Ching Man , Keilwagen Jens , Pardo Jeremy 
Published: November 2018

AbstractOropetium thomaeum is an emerging model for desiccation tolerance and genome size evolution in grasses. A draft genome of Oropetium was recently sequenced, but the lack of a chromosome‐scale assembly has hindered comparative analyses and downstream functional genomics. Here, we reassembled Oropetium, and anchored the genome into 10 chromosomes using high‐throughput chromatin conformation capture (Hi‐C) based chromatin interactions. A combination of high‐resolution RNAseq data and homology‐based gene prediction identified thousands of new, conserved gene models that were absent from the V1 assembly. This includes thousands of new genes with high expression across a desiccation timecourse. Comparison between the Sorghum and Oropetium genomes revealed a surprising degree of chromosome‐level collinearity, and several chromosome pairs have near perfect synteny. Other chromosomes are collinear in the gene rich chromosome arms but have experienced pericentric translocations. Together, these resources will be useful for the grass‐comparative genomic community and further establish Oropetium as a model resurrection plant.
Isotopic evidence for Holocene January air temperature variability on the East Chukotka Peninsula
Authors: Vasil'chuk Yurij K. , Budantseva Nadine A. , Farquharson Louise M. , Maslakov Alexey A. , Vasil'chuk Alla C. , Chizhova Julia N. 
Published: November 2018

AbstractIce‐wedge ice can provide a valuable record of past winter climate variability at high latitudes yet to date this proxy has been applied only sparsely across the Chukotka region of Siberia. Here we present data from eight ice‐wedges at four sites across a 600‐km transect in Eastern Chukotka which we use to reconstruct regional Holocene winter paleotemperature. The Holocene age of ice‐wedges was established by radiocarbon dating of peat surrounding individual ice‐wedges. Our suite of radiocarbon ages suggests that the studied ice‐wedges formed between 12.9–11.8 and 3–2.5 cal ka BP. δ18O and δ2H values from ice‐wedge ice indicate that in coastal areas of Eastern Chukotka mean January air temperatures in the first half of the Holocene were on average 2–3°С lower than modern values, a finding that corresponds well with winter climate reconstructions for the other areas of Beringia. The variability of reconstructed mean January air temperatures in the study area did not exceed 2–4°C during the early to mid‐Holocene.
Use of a Reverberation Technique to Determine Grüneisen Parameter of Unreacted Plastic bonded Explosive
Authors: Qin Shuang , Zhang Xu , Liu Jun Ming , Yu Zheng , Zhao Feng , Zhang Rong , Zhong Bin 
Published: November 2018

AbstractGrüneisen parameter plays an important role in describing states of materials, especially for highly condition‐depended detonation process. Herein, this key empirical parameter of unreacted plastic bonded explosive (PBX) is determined by a multiple shock‐reverberated technique. The explosive sample is sandwiched between copper plates, within which, shock waves reverberate and compress explosive continuously. This rapid dynamic process is verified by the stepped pressures measured by embedded PVDF stress gauges. The Mie‐Grüneisen equation of state is utilized to calculate the off Hugoniot reverberated states, which have a better agreement with experiments compared to the impedance match method. Five shots of such explosive produce enough experimental data to fit the most reliable Grüneisen parameter. This work demonstrates the accuracy of the shock‐reverberated technique applied to insensitive explosive to determine Grüneisen parameter and provides reference for further study on unreacted JB‐9014.
γ‐Cyclodextrin Nitrate/CL‐20 Complex: Preparation and Properties
Authors: Maksimowski Paweł , Grzegorczyk Agnieszka , Cieślak Katarzyna , Gołofit Tomasz , Chmielarek Michał , Tomaszewski Waldemar , Pawłowski Wojciech 
Published: November 2018

AbstractGamma‐cyclodextrin/CL‐20 (γ‐CD/CL‐20) inclusion complex was prepared and characterized by infrared and 1H NMR spectroscopy. The study findings point to a weak interaction of CL‐20 protons with H3 and H5 protons, however relatively intense interactions with OH groups in a γ‐CD molecule, which allows to conclude that a CL‐20 molecule does not penetrate completely the toroid cavity formed by γ‐CD. The resulting complex was subject to nitration with 100 % nitric acid. Analysis of the 1H NMR spectrum demonstrated that an average of 21.6 OH groups in the starting γ‐CD underwent esterification. The nitrated complex is characterized by a higher density (1.81 g/cm3), and the sensitivity to impact (5 J) and friction (200 N) comparable to the starting complex. Thermogravimetric analysis of the nitrated γ‐Cyclodextrin/CL‐20 complex pointed to a two‐phase decomposition at 194 and 339 °C with a total significant exothermic effect amounting to 3230 J/g.