bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growth and saponin production of Talinum paniculatum Gaertn. callus culture on various addition with 2,4-dichlorophenoxy acetic acid (2,4-D) and kinetin
Authors: WARDANI DIAN PRAMITA , SOLICHATUN SOLICHATUN , SETYAWAN AHMAD DWI 
Published: July 2018

The objectives of the research were to study the effect of 2,4-dichlorophenoxiacetic acid (2,4-D) and kinetin on callus growth and saponin production from Talinum paniculatum Gaertn. callus. The outline of the research was T. paniculatum as a medicinal plant potentially to be developed with in vitro culture method. The addition of 2,4-D and kinetin in cultureâ€™s medium would induce protein synthesis, further more it would influence cell proliferation and cell metabolism with regulation enzyme action, so that both of induced callus growth and secondary metabolism production from the cell that be cultured. The research used factorial completely randomized design with two factors (2,4-D concentration: 0 mgl-1, 0.5 mgl-1, 1 mgl-1, 1.5 mgl-1 and kinetin concentration: 0 mgl-1, 0.5 mgl-1, 1 mgl-1, 1.5 mgl-1) with three replicates. Data that be collected were qualitative data (callus morphology included textur and colour of callus) and quantitative data (callus growth rate, callus wet weight, callus dry weight and saponin content). Data were analyzed by using anova and followed by DMRT 5% confidence level and corelation regresion. The result of the research indicated that the treatment with addition plant regulation (2,4-D and kinetin) on MS medium have significant effect on callus growth and saponin production. Addition 2,4-D 1.5 mgl-1 and kinetin 1.5 mgl-1 as anoptimum combination concentration to induce callus growth and saponin production.
The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.)
Authors: MARLIANA SOERYA DEWI , SURYANTI VENTY , SUYONO SUYONO 
Published: July 2018

The phytochemical screenings and analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.) with Thin Layer Chromatography (TLC) has been carried out. Isolation was done by Soxhlet extraction for 6 hours with petroleum ether and the residue was extracted by maceration during 24 hours with ethanol.The isolated compounds in ethanol extract were identified by phytochemical screenings method and TLC. The result showed the presence of alkaloid, saponin, cardenolin/bufadienol and flavonoid.
The adsorption of Remazol Yellow FG in the textile wastes by cogon grass (Imperata cylindrica (L.) Raeush)
Authors: KUSUMANINGSIH TRIANA , MASYKUR ABU , SUPRIYANTO RONI 
Published: July 2018

Research of application of cogon grass (Imperata cylindrica (L.) Raeush) for adsorption of Remazol Yellow FG dye was carried out. This experiment was done to get the optimal condition of adsorption of Remazol Yellow FG dye by cogon grass. Adsorption process was performed by variety soaking activation times of cogon grass in NaOH solution were in 0, 6, 12, 18, 24, 30, and 48 hours. The pH variation was 9, 10, 11, 12, and 13. The variation of contact times at 0, 20, 40, 60, 80, 100, and 120 minutes. The interactions between Remazol Yellow FG dye in solution and cogon grass were conducted in batch method. The surface area characteristic of cogon grass was analyzed by methylene blue method and the functional groups in the cogon grass were analyzed by infra red spectroscopy. The analysis of adsorption of textile dye was by Ultraviolet-Visible Spectroscopy. The results showed that the optimum condition from adsorption process of Remazol Yellow FG dye by cogon grass was reached at 24 hours of activation times, pH 10, and 60 minutes of contact time. Adsorptive capacity of active cogon grass to the textile dye was 5.165 mg/g while to the textile dye waste was 7.851 mg/g.
The antipyretic effect of basil leaf extract (Ocimi sancti folium) in rats
Authors: WIDIASTUTI ANDINA , MARIYAH YUL , SUHARSONO SUHARSONO 
Published: July 2018

Basil leaves are believed by community to reduce the fever. This study aimed to determine the effect of antipyretic and the effectiveness of basil leaf extract (Ocimi sancti folium) in rats. This research was a laboratory experimental research by using a random sampling technique. The experimental animal used was Wistar rats aged less than 2 months, as much as 20 rats divided into 4 groups. Group 1 as a negative control group was given aquadest of 2.5 ml, while groups 2, 3 and 4 as test groups were each given basil extract by 3.15 mg/100 gr BB, 6.30 mg mg/100 gr BB and 12.60 mg/100 gr BB, respectively, dissolved in 2.5 ml of aquadest. To increase the temperature, it was injected vaccine DPT 0.2 cc intra-muscular. The temperature measurement is conducted by using a digital thermometer. The data obtained were analyzed by using Statistical Package for Social Sciences 11.5 (SPSS 11.5) with Univariate Analysis of Variance method followed by Post Hoc test. The result of one factor analysis from Univariate Analysis of Variance test showed that the average of rats rectal temperature was significantly different for each test group and for each time range of temperature measurement, each of them was 0.034 and 0.005 with a significant level (Î±) = 0.05. From the result of analysis by using Post Hoc Test, there was a significant difference between dose group of 3.15 mg/100 gr BB with a dose group of 12.60 mg/100 gr BB and between the time of temperature measurements of 120 minutes and 180 minutes. At first dose and 180th minute, it was obtained a lowest rectal rats temperature. The results showed that the basil leaf extract had an antipyretic effect on rats. The optimal effectiveness evidenced by a lowest average rectal temperature was achieved by a dose of 3.15 mg/100 gr BB at 180th minute.
Processing soy sauce from golden apple snail (Pomacea canaliculata) by koji fermentation and addition of pineapple extract (Ananas comosus)
Authors: BADRIAH ELA LAILATUL , SURANTO SURANTO , PURWOKO TJAHJADI 
Published: July 2018

Badriah EL, Suranto, Purwoko T. 2008. Processing soy sauce from golden apple snail (Pomacea canaliculata) by koji fermentation and addition of pineapple extract (Ananas comosus). Biofarmasi 6: 1-7. Pomacea canaliculata had high protein content. Pomacea canaliculata could be used as a raw material in the production of soy sauce. Soy sauce is a liquid product made by fermentation or enzymatic. The addition of enzyme in the production of soy sauce can shorten the moromi fermentation process and increase nutrition improve. Bromelain was one of enzyme used in the production of soy sauce. This enzyme can be found in pineapple. The aims of the research were to determine the nutrition value of Pomacea soy sauce including carbohydrate, proteins and lipids contents and to determine the optimal pineapple extract rate in the production of Pomacea soy sauce by koji fermentation. The research was conducted through two phases, which were koji fermentation and continued hydrolysis by using the pineapple extract concentration variation [3:1, 3:2 and 3:3 (koji: pineapple extract)]. Pomacea canaliculata was fermented with Aspergillus oryzae for 7 days until producing koji. Koji was soaked in a salt solution of 20% (1:4, b/v) and added pineapple extract with concentration variation of (3:1, 3:2 and 3:3) for 3 days until it produced moromi. Moromi filtrate with extract pineapple variation (3:1, 3:2 and 3:3) was analyzed for the value of carbohydrate (sugar and starch), protein and lipids. Moromi filtrate was added by the spices and would produce Pomacea sauce. Finally, Pomacea sauce was tested preferably including the flavor, aroma, and color. Preferable test was analyzed by non-parametric statistic with Friedman Test and followed with Wilcoxon Ranking Method. The results of research showed that the value of sugar reduction on Pomace canaliculata sauce with pineapple extract variation of 3:1, 3:2 and 3:3 were 94.0849 mg/g, 132.5846 mg/g and 172.7485 mg/g, respectively. The starch value of Pomacea sauce with pineapple extract variation of 3:1, 3:2 and 3:3 were 35.8643 mg/g, 48.7123 mg/g and 52.5068 mg/g, respectively. The value of proteins content which dissolved on Pomacea sauce with pineapple extract variation of 3:1, 3:2 and 3:3 were 1230.196 mg/g, 1365.891 mg/g and 1475.016 mg/g, respectively. The value of lipids on Pomacea sauce with pineapple extract variation of 3:1, 3:2 and 3:3 were 4.1333 mg/g, 6.9333 mg/g and 7.1333 mg/g, respectively. The data preferable test, aroma and color on Pomacea sauce with pineapple extract variation of 3:3 were preferred than pineapple extract variation of 3:1 and 3:2. If the comparison with commercial sauce, Pomacea sauce was still less be taken a fancy to taste, flavor, and color.
The effect of ethanol extract of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress
Authors: HAMIDI BAARID LUQMAN , SAMIGUN SAMIGUN , LESTARI ANIK 
Published: July 2018

Hamidi BL, Samigun, Lestari A. 2010. The effect of extract ethanol of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress. Biofarmasi 8: 11-16. The aim of this research was to investigate the effects of extract ethanol of pegagan (Centella asiatica) after treated with restraint stress by measuring the eight arms radial maze performance of rats. Pre-test and post-test controlled groups design was applied in this research. Male Wistar rats (Rattus novergicus) with the mean age of 8 weeks and the body weight of 150-200 grams which used for sample were divided randomly into 4 groups, each group consisted of 6 rats, i.e. (i) control group (without any treatment), (ii) stress group (it was given by restraint stress for 2 hours/day for each rat), (iii) pegagan group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan), and (iv) pegagan and stress group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan and restraint stress for 2 hours/day for each rat). The treatments were given for 21 days. Within 12 days for each pre-treatment and post-treatment, a test on the eight arm radial maze was conducted on individual rat to observe its performance. The assessment of rat performance in the eight arms radial maze test was conducted based on error type B. Kruskal-Wallis and Mann-Whitney tests with SPSS for Windows 16 version were applied to analyze statistically the difference between four groups. Kruskal-Wallis test was used to show the significant performance level difference between four groups of rats with p=0.001, while Mann-Whitney test was used to determine the significant difference between stress group and pegagan group (p=0.001), also stress group and pegagan and stress group (p=0.001). The result of research showed that there was no significant difference between control group and stress group (p=0.051), control group and pegagan group (p=0.143), control group and pegagan and stress group (p=0.143), also pegagan group and pegagan and stress group (p=0.952). It was concluded that extract ethanol of pegagan improved the performances of rats on the eight arms radial maze after treated with restraint stress.
The effect of red pomegranate (Punica ganatum) peel extract on leukocyte number and type in rats exposed with mobile phone electromagnetic radiation
Authors: RAKHMAWATI MULKI , QADRIYATI ISNA , WIJAYANTI LILIK 
Published: July 2018

Rakhmawati M, Qadriyati I, Wijayanti L. 2011. The effect of red pomegranate (Punica granatum) peel extract on leukocyte number and type in rats exposed with mobile phone electromagnetic radiation. Biofarmasi 9: 55-61. This research aimed to examine the effect of red pomegranate (Punica granatum) peel extract on leukocyte number and type in rats exposed with mobile phone electromagnetic radiation. This study was a laboratory experimental post-test only control group design. The subjects used were 32 male rats, divided into 4 groups: (i) control group, (ii) mobile phone electromagnetic radiation-exposed group for 4 hour/day for 14 days, (iii) mobile phone electromagnetic radiation-exposed group for 4 hour/ day for 14 days with red pomegranate peel extract 50 mg/kg weight body in pre and during exposed, and (iv) mobile phone electromagnetic radiation-exposed group for 4 hour/day for 14 days with red pomegranate peel extract 50 mg/kg body weight pre, during and post-exposed. After 41 days, blood was collected in a clean tube with EDTA from orbital sinus of rats. Blood was used for leukocyte number and type in Pathology Clinic Laboratory, Faculty of Medicine, Sebelas Maret University. The data obtained were statistically analyzed with independent t-test by using SPSS Program for Microsoft Windows release 16.0 with a significance level at p<0.05. The results showed that red pomegranate peel extract decrease the leukocyte number than only exposed to mobile phone electromagnetic radiation group. The treatment of red pomegranate peel extract decreased for eosinophil, lymphocyte and monocyte, while for neutrophil, the treatment of red pomegranate peel extract showed increase than only exposed with mobile phone electromagnetic radiation group. Statistical analysis with independent t-test showed that the result was significant between group 1 and 3, and between group 2 and 3 of leukocyte number, but no significant for other groups. Meanwhile, the result of independent t-test showed no significant between all groups of the type of leukocyte. The experiment result showed that red pomegranate peel extract can significantly decrease the leukocyte number in rats exposed with mobile phone electromagnetic radiation (p<0.05), but no significant for different count (p>0.05).
Investigating the degrading properties of three different strains of fungi on commonly used pesticides in Guyana
Authors: FAREED REHANA , ANSARI ABDULLAH , SEECHARRAN DIANA , MUNROE LESLIE 
Published: July 2018

Fareed R, Ansari A, Seecharran D, Munroe L. 2017. Investigating the degrading properties of three different strains of fungi on commonly used pesticides in Guyana. Biofarmasi (Rumphius J Nat Prod Biochem) 15: 5-14. Bioremediation is the use of microbes to remove various contaminants from the environment. The present research work was carried out during 2014-15. Aspergillus niger, Aspergillus flavus and Penicillium spp. were employed for biodegradation of pesticides commonly used in Guyana. Initially, the fungi were screened for ligninolytic potential by observing decolorization/degradation of a synthetic dye (Remazol Brilliant blue) in PDA agar. The degradation of dyes was noted by the change in original colour of the dye and visual disappearance of colour from the fungustreated Petri plates. In addition, accumulation of the dye by the fungal mycelium was also noticed. The fungi were then tested for their tolerance to the pesticides Diuron, Malathion and Diazinon respectively. This was done on solid media using PDA agar with amoxillin to prevent bacterial growth. The pesticides were introduced to the fungi by way of disc diffusion. Discs of a known diameter were infused with the respective pesticides at different concentrations and placed at strategic points around the inoculated fungal mycelium. The extent of inhibition was measured by comparing the growth diameter in the sample verses growth in the control. The pesticide diazinon was found to have inhibited the fungi more when compared to Malathion and Diuron. Finally, the fungi were used to degrade the pesticides in a liquid culture-Peptone Broth. The pesticide Diuron was selected because the fungi thrived best in all concentrations of this pesticide. A concentration of the pesticide Diuron was place in flasks containing the peptone broth with mycelia discs of the respective fungal strain. The final concentration of the pesticide was determined by using High Performance Liquid Chromatography (HPLC). The concentration of the pesticide in each strain of fungi was decreased. Aspergillus niger was found to have the highest rate of pesticide degradation followed by Aspergillus flavus and Penicillium.
Ubijanje neodvisne scene ali zakaj potrebujemo center sodobnih scenskih umetnosti
Authors: Jablanovec Bojan 
Published: July 2018

Abstract
Pobuda in poziv k ustanovitvi Centra sodobnih scenskih umetnosti je bila prvič javno predstavljena 7. junija 2017 na tiskovni konferenci ob podaljšanju pogodbe med Ministrstvom za kulturo, Elektrom Ljubljana in zavodom Bunker za upravljanje prizorišča Stara mestna elektrarna Elektro Ljubljana, potem pa še 26. junija v intervjuju za časopis Dnevnik (Elektrika in kurjava ne zadostujeta več, (www.dnevnik.si/1042776235/kultura/oder/bojan-jablanovec-gledaliski-reziser-elektrika-inkurjava-ne-zadostujeta-vec) in 7. novembra na spletni strani Asociacija (www.asociacija.si/si/2017/11/07/kulturna-premisljevanja-bojan-jablanovec/).
Skupna izjava plenuma na področju sodobne uprizoritvene umetnosti
Authors: Marinič Alenka , Zorec Aleš , Lobnik Alja , Podrzavnik Andreja , Kolenc Bara , Korun Barbara , Krnetić Benjamin , Pogačar Božidar , Srhoj Dejan , Alfirević Dragana , Semič Eva , Bogdanovski Goran , Kamnikar Gregor , Barši Izidor , Mijačević Ivan , Jevtović Jana , Založnik Jasmina , Risba Jatun , Kastelic Jernej , Žabkar Jonas , Kraner Kaja , Lorenci Kaja , Pejović Katarina , Legin Katja , Teržan Kaja , Jurišić Leja , Kalcina Liana , Erdani Luka , Reiter Magdalena , Vrhovac Maja , Lapajne Maja Dekleva , Kline Mala , Uršič Manca , Bučar Mateja , Rebolj Mateja , Arhar Nika , Leskovšek Nika , Lampič Eva Nina , Hazabent Petra , Brezavšček Pia , Centa Primož , Pernarčič Radharani , Bobnič Robert , Bozovičar Rok , Vevar Rok , Lunaček Sarah , Rakef Saška , Stone Sunčan , Koncut Suzana , Benčan Tana , Tanko Petra , Reba Teja , Dobaj Tina , Janežič Tina , Valentan Tina , Dekleva Tomaž Lapajne , Vohar Urška , Brodar Urška , Sajko Urška , Kranjčan Žigan 
Published: July 2018

Abstract
Puertos atlánticos y mediterráneos en tiempos de Trajano
Authors: Bermejo Meléndez Javier , Campos Carrasco Juan M. 
Published: July 2018

Resumen. Con el ascenso de Trajano al poder en las postrimerías del s. I d. C. el estado conocerá un profundo proceso de reformas tendentes a mejorar la delicada situación administrativa, y especialmente financiera, heredadas del último emperador flavio. En este contexto las medidas puestas en marcha por el emperador hispano tendentes a reforzar el aparato de suministros estatales, la annona, hacia los mercados urbanos y el limes del imperio supondrá el desarrollo de una importante provincialización económica, así como un aumento del intervencionismo estatal. En línea con esta argumentación recogemos en el siguiente trabajo una docena de enclaves portuarios que jalonaron las principales rutas de abastecimiento y en los que se aprecia un importante desarrollo de su topografía, con nuevos espacios de almacenamientos, aumento del volumen de mercancías, etc. al socaire de esta nueva etapa comercial. Palabras clave: puertos, stationes, Ostia, portus Traiani, annona.
On the moving plane method for boundary blow-up solutions to semilinear elliptic equations
Authors: Canino Annamaria , Sciunzi Berardino , Trombetta Alessandro 
Published: July 2018

Abstract
We consider weak solutions to

-

Δ
⁢
u

=

f
⁢

(
u
)

{-\Delta u=f(u)}

on

Ω
1

∖

Ω
0

{\Omega_{1}\setminus\Omega_{0}}

, with

u
=
c
≥
0

{u=c\geq 0}

in

∂
⁡

Ω
1

{\partial\Omega_{1}}

and

u
=

+
∞

{u=+\infty}

on

∂
⁡

Ω
0

{\partial\Omega_{0}}

, and we prove monotonicity properties of the solutions via the moving plane method.
We also prove the radial symmetry of the solutions in the case of annular domains.
Morphological and molecular characterization of Paramphistomum epiclitum of small ruminants
Authors: Jadav Mehul M. , Kumar Niranjan , Das Bhupamani , Solanki Jaesh B. 
Published: July 2018

AbstractMorphological and molecular identification can pave the way to design the most effective control measures against theParamphistomum epiclitumin small ruminants. Morphology of the flukes had described the features ofParamphistomumgenus. Body was conical with concave ventral and convex dorsal surface, tegumental spines all around the body in the immature stage, terminal funnel shape oral sucker, sub-terminal acetabulum, blind caeca with a serpentine course touching the anterior level of the acetabulum. Vitelline glands were at the lateral margins of the body extended from the pharynx to the posterior sucker. Testes were lobed and tandem, wavy post-testicular uterus and genital pore behind intestinal bifurcation. Sequence analyses of internal transcribed spacer (ITS)-2+ (PCR products of approximately 500 bp) of 10 flukes yielded 2 genotypes, Navsari isolate 1 and 2. In BLAST analysis, ITS-2+ genotypes were 97.3–99% similar with published sequences (KF564870, JF834888, KF642983 and JX678254) ofP.epiclitumof Paramphistomatidae. Two genotypes depicted 4 single nucleotide polymorphisms (NPs) in the form of transitions (C-T at 10 and 18; G-A at 255; A-G at 367 locus), 1 triple NPs (CGT-GAA between 21–23 loci) and missing A base at codon 40 in the genotype 1. Average AT and GC content was 49.61% and 50.38%, respectively. Trees topology inferred by Neighbor Joining and Maximum Likelihood methods of ITS2+ of trematodes were similar, with small difference of bootstrap values. Navsari genotypes formed a tight cluster with theP.epiclitum, originated from different location with high bootstrap value and 0.004–0.011 estimated evolutionary divergence.
Further study on Procamallanus (Spirocamallanus) pintoi (Kohn et Fernandes, 1988) (Nematoda: Camallanidae) in Corydoras paleatus and Corydoras micracanthus (Siluriformes: Callichthyidae) from Salta, Argentina, with a key to congeneric species from Neotropical Realm
Authors: Ailán-Choke Lorena G. , Ramallo Geraldine , Davies Dora 
Published: July 2018

Abstract
This study describes the morphology of Procamallanus (Spirocamallanus) pintoi (Kohn et Fernandes, 1988), isolated from the intestine of Corydoras micracanthus Regan, 1912 and C. paleatus (Jenyns, 1842) (both Callichthyidae, Siluriformes) from Bermejo and Juramento River basins, respectively, province of Salta, Argentina. This is the first record of P. (S.) pintoi in C. micracanthus from Northwestern Argentina. The light and scanning electron microscopy observations of P. (S.) pintoi, revealed a few previously unreported morphological details of the cephalic region, vulva and caudal region of males. In addition, morphometric variability was observed in, mainly body size, glandular oesophagus length and position of the vulva between present specimens and those described in previous studies. A key to the species of Procamallanus (Spirocamallanus) from the Neotropical Realm is proposed.