bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-Cell RNA Sequencing of Visceral Adipose Tissue Leukocytes Reveals that Caloric Restriction Following Obesity Promotes the Accumulation of a Distinct Macrophage Population with Features of Phagocytic Cells
Authors: Weinstock Ada , Brown Emily J. , Garabedian Michela L. , Pena Stephanie , Sharma Monika , Lafaille Juan , Moore Kathryn J. , Fisher Edward A. 
Published: July 2019

Abstract
Obesity can lead to type 2 diabetes and is an epidemic. A major contributor to its adverse effects is inflammation of the visceral adipose tissue (VAT). Life-long caloric restriction (CR), in contrast, results in extended lifespan, enhanced glucose tolerance/insulin sensitivity, and other favorable phenotypes. The effects of CR following obesity are incompletely established, but studies show multiple benefits. Many leukocyte types, macrophages predominantly, reside in VAT in homeostatic and pathological states. CR following obesity transiently increases VAT macrophage content prior to resolution of inflammation and obesity, suggesting that macrophage content and phenotype play critical roles. Here, we examined the heterogeneity of VAT leukocytes and the effects of obesity and CR. In general, our single-cell RNA-sequencing data demonstrate that macrophages are the most abundant and diverse subpopulation of leukocytes in VAT. Obesity induced significant transcriptional changes in all 15 leukocyte subpopulations, with many genes showing coordinated changes in expression across the leukocyte subpopulations. Additionally, obese VAT displayed expansion of one major macrophage subpopulation, which, in silico, was enriched in lipid binding and metabolic processes. This subpopulation returned from dominance in obesity to lean proportions after only 2 weeks of CR, although the pattern of gene expression overall remained similar. Surprisingly, CR VAT is dominated by a different macrophage subpopulation, which is absent in lean conditions. This subpopulation is enriched in genes related to phagocytosis and we postulate that its function includes clearance of dead cells, as well as excess lipids, contributing to limiting VAT inflammation and restoration of the homeostatic state.
Prediction of Shale-Gas Production at Duvernay Formation Using Deep-Learning Algorithm
Authors: Lee Kyungbook , Lim Jungtek , Yoon Daeung , Jung Hyungsik 
Published: July 2019

SummaryDecline–curve analysis (DCA) is an easy and fast empirical regression method for predicting future well production. However, applying DCA to shale–gas wells is limited by long transient flow, a unique completion design, and high–density drilling. Recently, a long short-term-memory (LSTM) algorithm has been widely applied to the prediction of time–series data. Because shale–gas–production data are time–series data, the LSTM algorithm can be applied to predict future shale–gas production. After information for 332 shale–gas wells in Alberta, Canada, is obtained from a commercial database, the data are preprocessed in seven steps, including cutoffs for well list, data cleaning, feature extraction, train and test sets split, normalization, and sorting for input into the LSTM model. The LSTM model is trained in 405 seconds by two features of production data and a shut–in (SI) period from 300 wells. The two–feature case shows a better prediction accuracy than both the one–feature case (i.e., production data only) and the hyperbolic DCA, where the three methods are tested on unseen data from 15 wells. The two–feature case can predict future production rates according to the SI period and provide a stable result for available time–series data.
Voluntary Blood Donation Practice and Associated Factors among Civil Servants in Chiro Town of Western Hararghe Zone, Eastern Ethiopia: a Cross-sectional study
Authors: Mijena Gelana Fekadu , Nigussie Sintayehu , Gobena Tesfaye , Mitiku Habtamu , Abdi Hassen 
Published: July 2019

Abstract
Background

Despite blood transfusion has been available for the past 200 years; there is still a significant shortage of blood and blood products especially in low and middle income countries. In 2016 national blood demand of Ethiopia was estimated to be one million units per year, while only 17.4% (173,930) units of blood collected in the same year. Out of 25,400 units of blood requirement per year in West Hararghe, only 2750 units (10.8%) was collected in 2016 and 80% of these were from Civil servants. The aim of the study was to assess voluntary blood donation practice and associated factors among Chiro Town’s civil servants in 2018.

Methods

A cross-sectional study was conducted on 383 civil servants selected by multistage sampling technique. Data were collected using self-administer and pre-tested questionnaire. Data was analyzed using SPSS version 20 and binary logistic regression was performed to assess the association between determinant factors and voluntary blood donation practice. Adjusted odds ratios (AOR) with its 95% confidence interval (CI) were estimated to identify factors associated with the outcome variables in the multivariable analysis. A P<0.05 and AOR with 95% CI were used to report significance and strength of multivariable association, respectively.

Results

The life -time voluntary blood donation practice among civil servants was 43.7%(38.3,49.3). Having willingness to donate, [AOR=2.54; 95%CI=1.39, 4.65], being knowledgeable about blood donation [AOR=3.32; 95%CI=2.02, 5.46] and listening to mass media [AOR=1.83; 95%CI=1.01, 3.35] were variables significantly associated with voluntary blood donation practice.

Conclusions

This study shows poor Voluntary Blood donation practice while, having knowledge, willingness and access to mass media are attributable factors for blood donation practice in the study area.
The study of Wilson Disease in pregnancy management
Authors: Yu Xu-En , Pang Min , Han Yong-Zhu , Yang Ren-Min , Gao Shan 
Published: July 2019

Abstract
Abstract
Background: Pregnancy management in women with Wilson disease (WD) remains an important clinical problem. This research was conducted to investigate how to avoid worsening of WD symptoms during pregnancy and increase pregnancy success in women with WD by identifying the best pregnancy management approaches in these patients.

Methods: The clinical data of 117 pregnancies among 75 women with WD were retrospectively analyzed. Related information of the fetus was also recorded and analyzed. At the same time, regression analysis was performed for data of 22 pregnant women without WD, as normal controls.

Results: Of a total of 117 pregnancies among the 75 women with WD and 31 pregnancies among the 22control womenincluded in this study, there were 108 successful pregnancies and 9 spontaneous abortions. Among the 108 successful pregnancies, 97 women a history of copper chelation therapy before pregnancy; all 97 women stopped anti-copper therapy during pregnancy. The nine women with spontaneous abortion had no pre-pregnancy history of copper displacement therapy. The incidence of lower limb edema was higher in the WD group than in normal controls (P=0.036). Compared with the control group, there was a higher proportion in the WD group of male infants (P=0.022) and lower average infant birth weight (t=3.514, P=0.001).

Conclusion: It is relatively safe for women with WD patients to become pregnant. The best management method for pregnancy in women with WD may be intensive pre-pregnancy copper chelation therapy and no anti-copper treatment during pregnancy.
A preliminary assessment of vital-signs-integrated patient-assisted intravenous opioid analgesia (VPIA) for postsurgical pain: a case series
Authors: SNG Ban Leong , Tan Daryl Jian'an , TAN Chin Wen , HAN Nian-Lin Reena , SULTANA Rehena , SIA Alex Tiong Heng 
Published: July 2019

Abstract
Background

We developed a Vital-signs-integrated Patient-assisted Intravenous opioid Analgesia (VPIA) analgesic infusion pump, a closed-loop vital signs monitoring and drug delivery system which embodies in a novel algorithm that takes into account patients’ vital signs (oxygen saturation, heart rate). The system aims to allow responsive titration of personalized pain relief to optimise pain relief and reduce the risk of respiratory depression. Moreover, the system will be important to enable continuous monitoring of patients during delivery of opioid analgesia.

Methods

Nineteen patients who underwent elective gynecological surgery with postoperative patient controlled analgesia (PCA) with morphine were recruited. The subjects were followed up from their admission to the recovery room/ ward for up to 24 hours until assessment of patient satisfaction on the VPIA analgesic infusion pump.

Results

The primary outcome measure of incidence of oxygen desaturation showed all patients had at least one episode of oxygen desaturation (<95%) during the study period. Only 6 (31.6%) patients had oxygen desaturation that persisted for more than 5 minutes. The average percentage time during treatment that oxygen saturation fell below 95% was 4.6%. Fourteen (73.7%) out of 19 patients encountered safety pause, due to transient oxygen desaturation or bradycardia. The patients’ median [IQR] pain scores at rest and at movement after post-op 24 hours were 0.0 [2.0] and 3.0 [2.0] respectively. The average ± SD morphine consumption in the first 24 hours was 12.5 ± 7.1mg. Majority of patients were satisfied with their experience with the VPIA analgesic infusion pump.

Conclusions

We have demonstrated the potential of VPIA analgesic infusion pump to increase the safety and patient satisfaction by incorporating vital signs monitoring to intravenous opioid analgesia.
Model Construction of Medical Endoscope Service Evaluation System-Based on the Analysis of Delphi Method
Authors: Zheng Jun , Lou Ligang , Xie Ying , Chen Siyao , Li Jun , Wei Jingming , Feng Jingyi 
Published: July 2019

Abstract
Background:
Medical endoscope is widely used in clinical practice for the purpose of diagnosis and treatment,occupying around 5% of the medical device market. Evaluating the true service level of medical endoscope is essential and necessary to improve overall performance of medical diagnosis and treatment, and to maintain competitiveness of endoscope manufacturers, however, such a tool is not available in the market. This study develops an Evaluation Index System (EIS) to assess service level of medical endoscope, and to provide suggestions for improving the service level through the Delphi method.

Methods:
Firstly, the possible factors influencing the service level were identified from literature review. In parallel, the Delphi expert method questionnaire was designed and 25 experts were invited to conduct three rounds of questionnaire, to evaluate and rate the possible factors. Finally, we determined the weights associated with the factors, using the analytic hierarchy process (AHP) and percentage method, and developed theservice level EIS.

Results:
The EIS consists of 3 first-level indicators, 24 second-level indicators and 68 third-level indicators. According to the weights computed using AHP, first-level indicators are ranked as post-sale (0.62), in-sale (0.25) and pre-sale (0.13). Through case verification, the medical endoscope brand Olympus had a total score of 4.17, Shanghai Aohua had a total score of 3.71, and Shanghai Chengyun had a total score of 3.28, which matches its market popularity and ranking in terms of market share. The results obtained from the EIS are consistent with the reality.

Conclusions:
The EIS established in this study is comprehensive, reliable and reasonable with strong practicality. The EIS can act as a tool for the endoscope users to evaluate potential products and make informed choices. It also provides a measurable basis for endoscope manufacturers and service providers to improve service quality.
The influence of depressive symptoms on the efficacy of a short-term group form of Schema Cognitive Behavioural Therapy for personality disorders: A naturalistic study
Authors: Koppers David , Van Henricus , Peen Jaap , Alberts Jet , Dekker Jack 
Published: July 2019

Abstract
Background
This naturalistic study looked at the effect of comorbid depressive symptoms on the outcomes – at treatment termination and three-month follow-up – of Short-Term Schema Cognitive Behavioural Therapy in groups for personality disorders.

Methods
We investigated 225 patients with personality disorders and comorbid depressive symptoms (PD-DEP) and patients without comorbidity (PD), focusing on symptom and schema severity and coping styles. We also measured the rate of symptom remission. The data obtained were subjected to multilevel analysis.

Results
Psychiatric symptoms and maladaptive schemas improved in both patient groups. Effect sizes were moderate. It proved more difficult to improve coping styles. Symptom remission was achieved in the minority of the total sample There were no differences in outcome between PD and PD-DEP at treatment termination. Psychiatric symptoms were more severe among PD-DEP patients at follow-up.

Conclusion
A short-term form of schema therapy in groups proved to be an effective approach for a broad group of patients with personality disorders, however, the majority of patients did not achieve symptom remission. This indicates that this type of short-term group therapy should be considered a valuable first step in a stepped-care model.
Management and outcome of pelvic fracture associated with vaginal injuries: a retrospective study of 25 cases
Authors: Li Pengyu , Zhou Dongsheng , Fu Baisheng , Song Wenhao , Dong Jinlei 
Published: July 2019

Abstract
Backgound: Pelvic ring fractures associated with vaginal injuries were rarely reported due to low incidence. We hope a retrospective study containing a relatively large number of patients would help improve clinical outcome.
Methods: We conducted a retrospective review of 25 patients with pelvic fractures associated with vaginal injury treated at our institution. The records of these patients were collected and 24 patients were followed-up for 10-36 months.
Results: All patients suffered anterior pelvic ring fracture. Disruption of anterior ring and Young-Burgess classification of bony fracture seemed to be related to severity vaginal injury. Gauze packing was done in 6 patients and 18 patients received surgical repair. Infection occurred in 6 patients among them 4 were due to delayed diagnosis. Factors associated with pelvic outcome were age, urethral injury, fixation strategy, infection and accuracy of reduction. Four patients suffered pain in sexual intercourse but no influence factor found correlated to sexual function due to small case series.
Conclusion: From our data, pelvic fractures caused by vertical shear forces and compromise pubic symphysis showed higher severity of vaginal injury. Age, associated urethral injury, fixation strategy and infection are prognostic factors of pelvic result. Significant relationship was shown between radiological and clinical outcomes. A thorough vaginal examination should be performed in female patients with pelvic fractures especially those with disruption of anterior pelvic ring to avoid missed diagnosis of vaginal laceration.
Key words: Pelvic fracture, Vaginal injury, Clinical outcome
New strategy of color and power doppler sonography combined with DMSA in the assessment of acute pyelonephritis in infants
Authors: Chen Min Guang , Yang Yan , Yang Qing , Zhuang Jie Qiu , Ye Xiao Hua , Zheng Wen Jie 
Published: July 2019

Abstract
Objectives

The purpose of this study was to evaluate the clinical value of color and power doppler sonography (CPDS) when combined it with 99mTc-dimercaptosuccinic acid scintigraphy (DMSA) in assessment of acute pyelonephritis (APN) in infants.

Methods

A total of 79 children with APN , including 52 males and 27 females, age range 1 month to 3 years old. All patients underwent laboratory inspection including urinary routine test, clean medium urine culture, urinary β2 microglobulin (β2-MG), urinary N-acetylglucosaminidase (NAG), blood routine test, C-reactive protein(CRP), renal function, serum electrolyte and urinary ultrasound. All 79 patients underwent CPDS and DMSA within 48 hours of fever.

Results

All 79 patients with fever. The peripheral blood routine showed elevated white blood cells in 75 cases, normal in 4 cases, CRP increased in 77 cases, normal in 2 cases. Urine β2-MG increased in 40 cases and normal in 39 cases. Urinary ultrasonography revealed 2 cases of renal cortical echo changes, 24 cases of kidney enlargement, and 1 left kidney shrinkage. Among 79 children, DMSA showed abnormal findings in 95 kidneys and CPDS, in 105 kidneys. The total sensitivity of CPDS was 69.4%, and the total positive predictive value was 62.8%. The sensitivity of CPDS under 6 months was 56.9%, which was 84.2% between 6 months to 1 year, and 94.4% from 1 to 3 years old, respectively. There was no significant correlation between CPDS levels and DMSA positive results. The abnormal rate of intermediate part in the kidneys was significantly lower than that in the upper and lower poles. Children with abnormal CPDS have a greater risk of renal scarring(p<0.05).

Conclusion

The sensitive of CPDS is highly age-related, it can as a non-invasive tool for assessment of acute pyelonephritis in infants, especially for infants more than 6 months old. CPDS is useful to be combined with DMSA to assess the APN of infants, for infants more than 6 months with febrile urinary tract infections, CPDS is the first recommendation, and followed by DMSA six months later to assess renal scar formation. For those less than 6 months, the DMSA assessment is still the preferred choice.
Gene expression profile of human T cells following a single stimulation of peripheral blood mononuclear cells with anti-CD3 antibodies
Authors: Sousa Isabel Garcia , Simi Kelly Cristina Rodrigues , Almo Manuela Maragno do , Bezerra Maryani Andressa Gomes , Doose Gero , Raiol Taina , Stadler Peter F , Hoffmann Steve , Maranhão Andrea Queiroz , Brigido Marcelo M 
Published: July 2019

Abstract
Background: Anti-CD3 immunotherapy was initially approved for clinical use for renal transplantation rejection prevention. Subsequently, new generations of anti-CD3 antibodies have entered clinical trials for a broader spectrum of therapeutic applications, including cancer and autoimmune diseases. Despite their extensive use, little is known about the exact mechanism of these molecules, except that they are able to activate T cells, inducing an overall immunoregulatory and tolerogenic behavior. To better understand the effects of anti-CD3 antibodies on human T cells, PBMCs were stimulated, and then, we performed RNA-seq assays of enriched T cells to assess changes in their gene expression profiles. In this study, three different anti-CD3 antibodies were used for the stimulation: two recombinant antibody fragments, namely, a humanized and a chimeric FvFc molecule, and the prototype mouse mAb OKT3.
Results: Gene Ontology categories and individual immunoregulatory markers were compared, suggesting a similarity in modulated gene sets, mainly those for immunoregulatory and inflammatory terms. Upregulation of interleukin receptors, such as IL2RA, IL1R, IL12RB2, IL18R1, IL21R and IL23R, and of inhibitory molecules, such as FOXP3, CTLA4, TNFRSF18, LAG3 and PDCD1, were also observed, suggesting an inhibitory and exhausted phenotype.
Conclusions: In conclusion, we used a deep transcriptome sequencing method for comparing three anti-CD3 antibodies in terms of Gene Ontology enrichment and immunological marker expression. The present data showed that both recombinant antibodies induced a compatible expression profile, suggesting that they might be candidates for a closer evaluation with respect to their therapeutic value. Moreover, the proposed methodology is amenable to be more generally applied for molecular comparison of cell receptor dependent antibody therapy.
Molecular analysis of Blastocystis sp. and its subtypes from treated wastewater routinely used for irrigation of vegetable farmlands in Iran
Authors: Javanmard Ehsan , Rahimi Hanieh Mohammad , Niyyati Maryam , Aghdaei Hamid Asadzadeh , Sharifdini Meysam , Mirjalali Hamed , Zali Mohammad Reza , Karanis Panagiotis 
Published: July 2019

Abstract
Treated wastewater samples were collected, filtered using sterile 47-mm cellulose nitrate membrane and DNA extracted from the filtered materials. The presence of Blastocystis sp. was confirmed via polymerase chain reaction (PCR) targeting the SSU rRNA gene of Blastocystis sp. in 5/12 of samples. Based on the subtype analysis after sequencing, 2, 2 and 1 of ST2, ST6 and ST8 were detected among the isolates, respectively. Furthermore, both ST6s were allele 139, alleles 11 and 138 were identified in ST2 and the only ST8 was allele 95. The phylogenetic tree showed that one of ST2 was clustered together with those ST2 that were already reported from humans and animals. The presence of Blastocystis sp. in treated wastewater can indicate the potential role of this type of water for irrigation in the transmission of pathogenic microorganisms to downstream farmlands.
Occurrence and molecular characterisation of Acanthamoeba isolated from recreational hot springs in Malaysia: evidence of pathogenic potential
Authors: Mohd Hussain Rosnani Hanim , Ishak Ahmad Razali , Abdul Ghani Mohamed Kamel , Ahmed Khan Naveed , Siddiqui Ruqaiyyah , Shahrul Anuar Tengku 
Published: July 2019

Abstract
This study aimed to identify the Acanthamoeba genotypes and their pathogenic potential in five recreational hot springs in Peninsular Malaysia. Fifty water samples were collected between April and September 2018. Physical parameters of water quality were measured in situ while chemical and microbiological analyses were performed in the laboratory. All samples were filtered through the nitrocellulose membrane and tested for Acanthamoeba using both cultivation and polymerase chain reaction (PCR) by targeting the 18S ribosomal RNA gene. The pathogenic potential of all positive isolates was identified using thermo- and osmotolerance tests. Thirty-eight (76.0%) samples were positive for Acanthamoeba. Water temperature (P = 0.035), chemical oxygen demand (P = 0.026), sulphate (P = 0.002) and Escherichia coli (P &lt; 0.001) were found to be significantly correlated with the presence of Acanthamoeba. Phylogenetic analysis revealed that 24 samples belonged to genotype T4, nine (T15), two (T3) and one from each genotype T5, T11 and T17. Thermo- and osmotolerance tests showed that 6 (15.79%) of the Acanthamoeba strains were highly pathogenic. The existence of Acanthamoeba in recreational hot springs should be considered as a health threat among the public especially for high-risk people. Periodic surveillance of hot spring waters and posting warning signs by health authorities is recommended to prevent disease related to pathogenic Acanthamoeba.