bluuebunny/crossref_metadata_2025_split huggingface dataset:
Features of endodontic treatment of teeth with curved root canals according to a survey of dentists
Authors: Rasskazov I. Y. , Makeeva M. K. , Karnaeva A. S. , Makhmudova Z. K. , Kozhevnikova L. A. , Pilshchikova O. V. 
Published: November 2024

Purpose: to identify difficulties of endodontic treatment of teeth with curved root canals according to a survey of dentists. Materials and methods: 90 dentists (26 men, 64 women) participated in the survey. 68 people had a therapeutic certificate, 19 people had an orthopedic certificate, 10 people had a surgical certificate, 6 people had a certificate in pediatric dentistry, and 43 people had a certificate in general dentistry (some respondents indicated that they had several certificates). The average age of respondents is 32 years (23–60). The interviewed dentists conduct practical work in public medical institutions, private dental clinics, and rented offices. The questionnaire included 20 questions (6 questions were aimed at collecting demographic data, 14 questions were aimed at obtaining information about the treatment of teeth with curved root canals). The questionnaire was submitted electronically to Google Forms. Statistical analysis of conjugacy tables using the Pearson chi-square test was performed using the program StatTech (Russia). Results: this survey reliably showed that dentists often experience difficulties in treating teeth with curved root canals. Dentists working in state institutions are significantly more likely to note that the rubberdam latex interferes (p= 0.049) and the clamp bow (p= 0.029) compared to doctors working in other institutions. Dentists working in a rented office are significantly more likely to report that a rubberdam latex interferes (p= 0.024) compared to doctors working in other institutions. Dentists who report difficult access when working with the mesial buccal canal of the lower molars are significantly more likely to note that a rubberdam latex interferes (p= 0.036). Dentists who use the approach «first negotiation to the working length – then coronal flaring» significantly more often experience difficulties when working with the mesial buccal first canal of the upper molars. Conclusions: the results of the survey demonstrated a number of differences in the performance of endodontic treatment of teeth with curved root canals. Based on the survey, statistically significant relationships were identified between some work features and difficulties encountered by doctors at the stages of treatment.
Evaluation of electrognathography indicators in patients with decreased height of the lower part of the face and symptoms of intolerance to the structural materials of dentures
Authors: Dubova L. V. , Rudakova A. M. , Samoylenko R. V. , Manin O. I. 
Published: November 2024

Purpose of the study: to conduct a comparative assessment of electrognathography indices in elderly patients with a decrease in the height of the lower part of the face with symptoms characteristic of intolerance to the structural materials of dentures, before and during the diagnostic and treatment stages of using occlusal-stabilizing devices. Material and methods. We examined 76 patients aged 61 to 74 years with a decrease in the height of the lower part of the face and the presence of symptoms characteristic of intolerance to the structural materials of dentures. Based on the results of a short “Hamburg” examination, 30 patients were selected with the possibility of dysfunctional disorders of the temporomandibular joint, for whom occlusal-stabilizing devices were made. Results. A study of elderly patients with a decrease in the height of the lower part of the face and symptoms characteristic of intolerance to the structural materials of dentures revealed the effectiveness of the use of occlusal-stabilizing devices at the treatment and diagnostic stages due to a decrease in complaints of dry mouth more than in twice, a decrease in the intensity of burning of the mucous membrane, a decrease in the number of complaints accompanying dysfunctional disorders of the temporomandibular joint, as well as an improvement in electrognathography indicators to physiological norms. Conclusion. The obtained results of treatment with occlusal-stabilizing devices for patients with a decrease in the height of the lower part of the face, presenting complaints characteristic of intolerance to the structural materials of dentures, at the treatment and diagnostic stages indicate the feasibility and effectiveness of their use in this category of people.
Review of domestic test systems for detecting current or past SARS‑CoV‑2 infection using enzyme‑linked immunosorbent assay
Authors: Galkina O. V. , Anpilova A. O. , Bogdanova E. O. , Zubina I. M. , Levykina E. N. 
Published: November 2024

Introduction. Rapid and reliable assessment of circulating antibody levels to SARS-CoV-2 remains the current challenge for monitoring and preventing the spread of COVID-19. As the risk of emergence and dissemination of new viral strains continues to grow, epidemiologic research is also becomes increasingly important. The enzyme-linked immunosorbent assay (ELISA) is useful to assess the vaccination effectiveness and the need for revaccination, the proportion of post-disease and vaccinated individuals, and to monitor population immunity. This article provides an overview of the local ELISA kits currently available for the detection of antibodies against SARS-CoV-2 viral proteins, with a comparison to the certified kit by Mediagnost Ltd. (Germany).The aim. Laboratory testing of seven domestic reagent kits for the determination of antibodies to SARS-CoV-2 viral proteins by ELISA in comparison with the certified test system “Anti-SARS-CoV-2 ELISA E 111-IVD” (Mediagnost Ltd., Reutlingen, Germany).Materials and methods. The study included 80 serum samples from conditionally healthy donors: experimental group (n=70, serum samples collected after 2019) and control group (n=10, serum samples collected before 2018). The experimental group is comprised of non-infected and post-disease individuals; a proportion of those were vaccinated against COVID-19.Results and conclusions. The data obtained indicate high reproducibility, sensitivity (100 %), and specificity for all domestic ELISA kits in detecting both post-disease (90–95 %) and vaccinated volunteers (100 %). The main quality criteria of the obtained results were comparable to those for the certified test system, “Anti-SARS-CoV-2 ELISA E 111-IVD” (Mediagnost Ltd., Germany). All kits detecting IgG to the receptor-binding domain (RBD) and to the S-glycoprotein were effective for the reliable assessment of antibody production after the vaccination. Kits for the detection of antibodies to the RBD and/or viral nucleocapsid are useful for identifying unvaccinated post-disease individuals.
HIV tropism to chemokine coreceptors. Features of the definition, the current state
Authors: Martynov M. A. , Semenov А. V. , Batyrgalieva L. M. , Levchenko M. A. 
Published: November 2024

HIV infection is a significant cause of death worldwide, the number of people with HIV infection in the Russian Federation as of 2022 amounted to 1163818 people. The determination of HIV tropism is necessary for the appointment of drugs from the group of penetration inhibitors, and also opens up new opportunities in the prediction and analysis of HIV infection in a patient.Aim. To present a description and assess the current state of methods for determining HIV tropism, to summarize known information about the influence of HIV tropism on the course of the disease, to identify topical issues related to HIV tropism and requiring solutions.Materials and methods. A review of domestic and foreign sources devoted to methods for determining the prevalence and clinical significance of HIV tropism was carried out.Results. For the effective administration of CCR5 antagonist drugs, preliminary analysis is necessary to establish the tropism of HIV by genotypic or phenotypic methods. The use of CCR5 antagonists is not possible if HIV can use the CXCR4 coreceptor. CXCR4 – tropism of HIV is associated with the duration of the disease, a decrease in the number of CD4 cells, AIDS, and is a negative prognostic factor. Human mutations affecting coreceptors can affect the course of infection and susceptibility to HIV.Conclusion. The determination of HIV tropism is a useful analysis, the importance of which will increase in connection with the development of new drugs from the group of penetration inhibitors. To increase the availability of HIV tropism analysis in the Russian Federation, the creation of genotypic test systems is required. To create proprietary algorithms used in genotypic analysis, as well as laboratory testing and development of new effective drugs from the group of penetration inhibitors, it is necessary to develop a phenotypic test system. The small study of the influence of other regions of the env gene on HIV tropism, the study of HIV tropism to alternative coreceptors are urgent issues that need to be addressed.
Comparing quality parameters of apheresis and pooled platelet concentrates
Authors: Bulanov A. Yu. , Makarov M. S. , Borovkova N. V. , Storozheva M. V. , Ponomarev I. N. , Evseev A. K. , Goroncharovskaya I. V. , Bitkova E. E. , Kostin A. I. 
Published: November 2024

Introduction. Harvesting of pooled platelet concentrates (PC) significantly expands the volume of transfusion support and could be used to create strategic platelet reserve.The aim of work. To compare the quality of pooled and apheresis platelet concentrates.Materials and methods. We studied platelet fractions (PF), isolated from the whole blood of cadre donors with Reveos system, platelet concentrates, obtained by pooling PF (Pooled PC), and platelet concentrates harvested by automatic apheresis. In all cases 2 days passed from the moment of blood donation to the platelet examination. In PC samples we studied cellular composition, morphofunctional parameters of platelets, pH and electrochemical parameters, hypotonic shock response.Results. The morphofunctional platelet rate in PF was significantly reduced, comparing to the normal levels. At the same time, the morphofunctional parameters of platelets did not significantly differ in PF and pooled PC. The pH values, the total content of platelets and leukocytes in the pooled PC corresponded to the requirements of The rules for procurement, storage, transportation and clinical use of donated blood and its components (2019). The total platelet count, the level of annexin-positive platelets, did not significantly differ in apheresis PC and pooled PC, hypotonic shock response was correct in apheresis PC and pooled PC. On the other hand, the level of platelets with granules in pooled PC was 1,8 times lower than in apheresis PC, other morphofunctional parameters of platelets in pooled PC were also reduced. Pooled PC were characterized by more positive level of open circuit potential and lower antioxidant capacity compared with apheresis PC on the same storage time.Conclusions. Platelet pooling with Reveos system is acceptable for obtaining PC doses that comply with Technical Regulations. Nevertheless, methods of pooled PC harvesting need further optimization.
Application of Raman spectroscopy using an original optical sensor to assess the laboratory efficacy of antiplatelet drugs
Authors: Moiseeva E. M. , Roytman A. P. , Rafalskiy V. V. , Zyubin A. Y. , Samusev I. G. , Filimonkina Iu. A. , Mikhailova L. V. 
Published: November 2024

The main method for monitoring the laboratory effectiveness of antiplatelet drugs in modern clinical practice is aggregometry, but this method is not without limitations. In this connection, there is an objective need to develop alternative methods. One of the promising areas is the method of Raman spectroscopy (RS).Objective: development of a method to detect high residual platelet reactivity (RPR) in patients with CVD receiving acetylsalicylic acid (ASA) or clopidogrel by giant Raman spectroscopy (GRS) using an original optical biosensor.Material sand Methods. Platelet-rich plasma of patients with cardiovascular diseases (CVD) was investigated by Raman spectroscopy using an original optical biosensor. Platelet aggregation activity was investigated using a Siemens PFA-200 aggregometer with three types of cartridges – Collagen/EPI, Collagen/ADP, and P2Y. Fisher’s linear discriminant analysis was performed using Statistica 13.0 package.Results. Raman spectra analysis using different values of frequency shifts (970 cm-1 or 1590 cm-1), allows to evaluate laboratory ineffectiveness separately for ASA and clopidogrel. Thus, the number of patients with high residual platelet reactivity (RPR) was 41.7 % ± 6.3 % with ASA and 36.7 % ± 6.2 % with clopidogrel therapy; similar values using aggregometry were 43.5 % ± 10.3 % and 30.4 % ± 9.6 %.Conclusion. Application of the method of Raman spectroscopy using the original optical biosensor allows to distinguish patients with high RPR in the population of patients with CVD receiving antiaggregant therapy.
Epidemiological features of chronic hepatitis B in the Russian Federation and the Samara region
Authors: Churbakovа O. V. , Akimkin V. G. , Pechkurov D. V. , Kindalova E. S. 
Published: November 2024

Research objective. To analyze the epidemiological features of chronic hepatitis in the Russian Federation and in the Samara region.Materials and methods. The data of official statistics were analyzed: the materials of the Federal Service for Supervision in the Field of Consumer Protection and Human Welfare, the Office of the Federal Service for Supervision in the Field of Consumer Protection and Human Welfare in the Samara Region.Results. The long-term dynamics of the incidence of newly diagnosed chronic hepatitis B in the Russian Federation and the Samara region in 1999–2021 was synchronous. In 1999–2001 the incidence of HCV (Chronic hepatitis B) in the Russian Federation and the Samara region had maximum values of 16,00⁄0000–22,50⁄0000. In the Samara region, it was higher than in the country. As a result of systematic measures to vaccinate the population against HCV during the period of mass immunization (2006–2021), the rate of decrease in the incidence of HCV in the Samara region decreased from 14,5 0⁄0000 to 2,40⁄0000 and in the country – from 14,0 0⁄0000 to 4,50⁄0000. The introduction of immunization of the child population against HCV in the Samara region had a positive effect on the incidence of HCV. From 2000 to 2021, the incidence of HCV decreased by 9,4 times. If in 2000 the incidence of children with HCV was 4,20⁄0000 in the Russian Federation, in 2002–7,30⁄0000 in the Samara region, then in 2021 in the Russian Federation it decreased to 0,080⁄0000, no cases were registered in the Samara region.
Features of laboratory indicators in cancer patients with COVID‑19, staying in the intensive care unit
Authors: Vlasenkova A. A. , Mochalova A. S. , Linnik D. V. , Akselrod B. A. , Alieskerov M. V. 
Published: November 2024

Objective: Comparative analysis of laboratory blood parameters in patients with COVID‑19 without and with oncopathology, who are in the intensive care unit.Materials and methods. A total of 118 people who had had COVID‑19 were examined. The main group consisted of 54 patients with various malignant neoplasms, the control group – 64 patients without oncological pathology. Laboratory parameters of a complete blood count (hemoglobin, leukocytes, lymphocytes, platelets) and a biochemical blood test (total protein, total bilirubin, urea, creatinine, ferritin) were examined.Results. Before COVID‑19 treatment, the average hemoglobin level in patients of the main group was 117.8±5.3 g/l, in the control group – 134.5±1.9 g/l (p=0.008), the average total protein level was 62.0±1.6 g/l and 67.9±0.9 g/l, respectively (p=0.005). After COVID‑19 treatment, hemoglobin in cancer patients remained lower (112.1±5.2 g/l) than in the control group (130.1±1.8 g/l, p=0.004), as well as total protein (60.0±2.0 g/l versus 66.3±0.9 g/l, p=0.004). Also, in patients of the main group after treatment, statistically significantly (p=0.0001) lower levels of lymphocytes (1.3±0.1 cells/ml) were found than in the control group – 2.0±0.1 cells/ml.Conclusion. Cancer patients had reduced levels of hemoglobin, total protein, and lymphocytes before and after treatment for COVID‑19. Increased urea, creatinine, and leukocytes were also noted, which requires enhanced monitoring of their condition.
Digital identification and adulteration analysis of Codonopsis Radix and Stellariae Radix based on the “digital identity” of chemical compositions
Authors: Wang Xianrui , Zhang Jiating , Jing Wenguang , Guo Xiaohan , Li Minghua , Cheng Xianlong , Wei Feng 
Published: November 2024

IntroductionUnder the background of digitalization of traditional Chinese medicine (TCM), this study aimed to realize the digital identification and adulteration analysis of Codonopsis Radix (CR) and Stellariae Radix (SR) based on chemical analysis.MethodsThis study combined digitalization concepts and chemical analysis and conducted a chemical analysis of CR and SR from different batches based on UHPLC-QTOF-MSE. Furthermore, the shared ions were extracted from different batches of CR and SR as their “ion characterization” after digital quantization. Then, the data matrices of unique ions of CR relative to SR and SR relative to CR were screened out, and the top-N ions were outputted as the “digital identities” of CR and SR, sorted by ionic strength. Finally, the above “digital identities” of CR and SR were used as benchmarks for matching positive samples and market samples to provide feedback on the matching credibility (MC) for identification and adulteration analysis.ResultsThe results showed that based on the “digital identities” of CR and SR, the digital identification of CR, SR, and positive samples can be realized at the individual level of TCM efficiently and accurately, even if 3% of SR in the mixed samples can still be identified efficiently and accurately. Moreover, 1 of the 12 batches of market samples was identified as an adulterated sample.ConclusionIt proved that the identification and adulteration analysis of two herbs can be realized efficiently and quickly through the “digital identities” of chemical compositions. It has important reference significance for developing the digital identification of CR and SR at the individual level of Chinese medicine based on the “digital identity” of chemical compositions, which was beneficial to the construction of digital quality control of CR and SR.
Ensemble modeling of SARS-CoV-2 immune dynamics in immunologically naïve rhesus macaques predicts that potent, early innate immune responses drive viral elimination
Authors: Byrne Catherine , Schiffer Joshua T. 
Published: November 2024

IntroductionAn unprecedented breadth of longitudinal viral and multi-scale immunological data has been gathered during SARS-CoV-2 infection. However, due to the high complexity, non-linearity, multi-dimensionality, mixed anatomic sampling, and possible autocorrelation of available immune data, it is challenging to identify the components of the innate and adaptive immune response that drive viral elimination. Novel mathematical models and analytical approaches are required to synthesize contemporaneously gathered cytokine, transcriptomic, flow cytometry, antibody response, and viral load data into a coherent story of viral control, and ultimately to discriminate drivers of mild versus severe infection.MethodsWe investigated a dataset describing innate, SARS-CoV-2 specific T cell, and antibody responses in the lung during early and late stages of infection in immunologically naïve rhesus macaques. We used multi-model inference and ensemble modeling approaches from ecology and weather forecasting to compare and combine various competing models.Results and discussionModel outputs suggest that the innate immune response plays a crucial role in controlling early infection, while SARS-CoV-2 specific CD4+ T cells correspond to later viral elimination, and anti-spike IgG antibodies do not impact viral dynamics. Among the numerous genes potentially contributing to the innate response, we identified IFI27 as most closely linked to viral load decline. A 90% knockdown of the innate response from our validated model resulted in a ~10-fold increase in peak viral load during infection. Our approach provides a novel methodological framework for future analyses of similar complex, non-linear multi-component immunologic data sets.
MICA and NKG2D gene polymorphisms influence graft survival, and response to therapy in kidney transplantation
Authors: Littera Roberto , Mocci Stefano , Argiolas Davide , Littarru Letizia , Lai Sara , Melis Maurizio , Sanna Celeste , Mereu Caterina , Lorrai Michela , Mascia Alessia , Angioi Andrea , Mascia Giacomo , Matta Valeria , Lepori Nicola , Floris Matteo , Manieli Cristina , Bianco Paola , Onnis Daniela , Rassu Stefania , Deidda Silvia , Carta Mauro Giovanni , Giuressi Erika , Perra Andrea , Chessa Luchino , Giglio Sabrina , Pani Antonello 
Published: November 2024

BackgroundAntibody-mediated rejection is a significant cause of kidney transplant failure. Recent studies have shown that the MHC class I MICA gene influences the transplantation outcome. However, the role of the primary MICA receptor, NKG2D, has yet to be explored.AimWe aimed to investigate the correlation between recipient/donor MICA allele matching and NKG2D genotype with the risk of antibody-mediated rejection and their potential clinical effects and implications for organ maintenance therapy.MethodsOf the 524 patients who underwent transplantation, 387 were eligible for the study. Complete MICA allele and two functional polymorphisms of NKG2D (rs1049174C&gt;G and rs2255336G&gt;A) were analyzed in 148 transplanted patients and 146 controls.ResultsIncreased recipient/donor MICA allele mismatches correlate with an elevated risk of antibody-mediated rejection (X2 = 6.95; Log-rank=0.031). Notably, the rs1049174[GG] genotype contributes to a significantly increased risk of antibody-mediated rejection (X2 = 13.44; Log-rank=0.001 and X2 = 0.34; Log-rank=0.84). The combined effect of two MICA allele mismatches and rs1049174[GG] genotype shows the highest risk (X2 = 23.21; Log-rank&lt;0.001). Most importantly, patients with rs1049174[GG] and rs2255336[AA] genotypes may respond less to mTOR inhibitor immunosuppressive therapy than Calcineurin inhibitors (rs1049174[GG]; P=0.035; and rs2255336[AA]; P=0.002).ConclusionRecipient/donor MICA allele mismatches and specific NKG2D variants, as well as their combinations, influence kidney transplant outcomes, providing insights for personalized treatment and enhancing graft survival.