bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations between brain morphology and motor performance in chronic neck pain: A whole‐brain surface‐based morphometry approach
Authors: De Pauw Robby , Coppieters Iris , Caeyenberghs Karen , Kregel Jeroen , Aerts Hannelore , Lenoir Dorine , Cagnie Barbara 
Published: June 2019

AbstractChanges in brain morphology are hypothesized to be an underlying process that drive the widespread pain and motor impairment in patients with chronic neck pain. However, no earlier research assessed whole‐brain cortical morphology in these patients. This case–control study assesses group‐differences in whole‐brain morphology between female healthy controls (HC; n = 34), and female patients with chronic idiopathic neck pain (CINP; n = 37) and whiplash‐associated disorders (CWAD; n = 39). Additionally, the associations between whole‐brain morphology and motor performance including balance, strength, and neuromuscular control were assessed. Cortical volume, thickness, and surface area were derived from high resolution T1‐weighted images. T2*‐weighted images were obtained to exclude traumatic brain injury. Vertex‐wise general‐linear‐model‐analysis revealed cortical thickening in the left precuneus and increased volume in the left superior parietal gyrus of patients with CINP compared to HC, and cortical thickening of the left superior parietal gyrus compared to HC and CWAD. Patients with CWAD showed a smaller cortical volume in the right precentral and superior temporal gyrus compared to HC. ANCOVA‐analysis revealed worse neuromuscular control in CWAD compared to HC and CINP, and in CINP compared to HC. Patients with CWAD showed decreased levels of strength and sway area compared to CINP and HC. Partial correlation analysis revealed significant associations between the volume of the precentral gyrus, and neuromuscular control and strength together with an association between the volume of the superior temporal gyrus and strength. Our results emphasize the role of altered gray matter alterations in women with chronic neck pain, and its association with pain and motor impairment.
Fat talk: Influences on body image in childcare
Authors: Lombardi Karen , Beatty Shelley , Devine Amanda , Wallace Ruth , Costello Leesa 
Published: June 2019

AbstractIssue addressedThis research explored the use of “fat talk” by early childhood educators and their awareness of their potential influence on the developing body image of young children.MethodsMixed methods comprising focus groups, telephone interviews and demographic surveys with 44 early childhood educators Australia‐wide.ResultsFindings showed many educators in this research recognised that they had a role to play in the development of children's body image, though some were unsure when body image began to develop. Educators engaged widely in “fat talk,” in the vicinity of children or other educators, and accepted such talk as normal.Discussion“Fat talk” features in the workplace and is commonly used by early childhood educators. Its influence on the development of body image on young children did not appear to be well understood by early years’ educators or of a concern to them.ConclusionsSince body image develops in children from around three years of age, the role of early childhood educators in its development should not be overlooked.So what? Relevance to Health PromotionBody image is a misunderstood public health concern, with long‐term impacts on weight, mental health and physical health. Promotion of positive body image and the early recognition of body image disturbance are crucial, as children who exhibit body dissatisfaction in their formative years have a greater risk of severe body image disturbance as they progress through childhood and adolescence. Since early childhood educators spend significant time with children, encouraging them to avoid engaging in “fat talk” and instead to promote positive body image is warranted.
Proflavine/acriflavine derivatives with versatile biological activities
Authors: Sabolova Danica , Kristian Pavol , Kozurkova Mária 
Published: June 2019

AbstractProflavine derivatives are extremely interesting chemotherapeutic agents, which have shown promising pharmaceutical potential due to their wide range of biological activities. This review summarizes the current state of research into the anticancer, antimicrobial, antimalarial and antileishmanial properties of these attractive compounds. Our attention has focused on new classes of proflavine conjugates, which display significant levels of anticancer activity. Highly promising cytotoxic properties have been identified in proflavine conjugates with imidazolidinones, ureas and thioureas. In particular, proflavine‐dialkyldithioureas displayed substantial cytotoxic effect against the human leukemia HL‐60 cells with IC50 values from 7.2 to 34.0 μm. As well, palladium complexes with proflavine ligand have important biologic activity. The LC50 values of these complexes were significantly lower than that of cisplatin against the SK‐BR‐3 cell line.
Potential self‐disinfection capacity of touch screen displays
Authors: Hessling Martin , Spellerberg Barbara , Hönes Katharina 
Published: June 2019

AbstractTouch screen displays are potential pathogen reservoirs and involved in the spread of hospital acquired infections. They emit visible light that is known for a weak but proven antimicrobial photoinactivation effect, so the question is whether displays have the potential to disinfect themselves. To test the antimicrobial capacity of touch screen displays, Staphylococcus carnosus are distributed on Samsung tablets and illuminated for up to 36 hours. The average number of colony forming units decreases with time with white light being the most efficient followed by blue, green and red light. Increasing the illumination intensity by a mirror leads to a faster bacterial decrease up to a 90% reduction in 15 hours. A 99.99% reduction of staphylococci should be possible by turning on the display over the weekend.
Regulatory effects of lncRNA ATB targeting miR‐200c on proliferation and apoptosis of colorectal cancer cells
Authors: Gao Zhenyuan , Zhou Hairong , Wang Yaping , Chen Juan , Ou Yimei 
Published: June 2019

AbstractThis investigation was intended to elucidate whether long noncoding RNA (lncRNA)‐activated by transforming growth factor‐β (ATB) interacting with miR‐200c could mediate colorectal cancer (CRC) progression, offering potential strategies for diagnosing and treating CRC. Here totally 315 patients with CRC were recruited, and their CRC tissues and adjacent normal tissues were gathered. Concurrently, four colon cancer cell lines (ie, SW620, Lovo, HCT116, and SW480) and the human colon mucosal epithelial cell line (NCM460) were also purchased. Moreover, si‐ATB, si‐NC, miR‐200c mimic, miR‐200c inhibitor, and miR‐NC were prepared for transfection into the CRC cells, and their effects on CRC cell lines were evaluated based on the conduction of 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, colony formation assay, and flow cytometry assay. Eventually, the Luciferase reporter gene assay was carried out to judge if there existed a targeted relationship between ATB and miR‐200c. The results of Cox regression analyses suggested that overexpressed lncRNA ATB, underexpressed miR‐200c, poor tumor differentiation, lymph‐vascular invasion, and perineural invasion were symbolic of shortened survival of the patients with CRC (all P < .05). Besides, transfection of pcDNA3.1‐ATB and miR‐200c inhibitor could boost the viability and proliferation of Lovo and SW620 cell lines (all P < .05). Meanwhile, the expressions of p53 and p21 were also reduced under treatments of pcDNA3.1‐ATB and miR‐200c inhibitor (P < .05). In addition, CDK2 seemed to reverse the contribution of miR‐200c to intensifying viability and proliferation of Lovo and SW420 cell lines (P < .05). Furthermore, ATB might downregulate miR‐200c expression by targeting it (P < .05), and CDK2 was subjected to dual regulation of both ATB and miR‐200c (P < .05). In conclusion, the lncRNA ATB/miR‐200c/CDK2 signaling was responsible for intensified proliferation and prohibited apoptosis of CRC cells, which might provide effective approaches for diagnosing and treating CRC.
LINC00324 accelerates the proliferation and migration of osteosarcoma through regulating WDR66
Authors: Wu Shaokun , Gu Zhiqian , Wu Yaojun , Wu Wei , Mao Bo , Zhao Shoujun 
Published: June 2019

AbstractOsteosarcoma (OS) is a type of malignancy featured with high morbidity and easy metastasis. Although past years have witnessed the great improvement in the treatments of OS, there remains a long way to go. Therefore, further research on the underlying molecular mechanism of OS progression is in imminent need. Long noncoding RNAs (lncRNA) are recognized as a cluster of transcripts over 200 bases. Increasing studies have unveiled their significant regulatory roles in cancers, including in osteosarcoma. Long intergenic non‐protein coding RNA 324 (LINC00324) is a newly identified lncRNA exerting oncogenic functions in several cancers, but its role in OS is yet to be uncovered. Therefore, the present study planned to explore the role of LINC00324 in osteosarcoma. We first validated the upregulation of LINC00324 in OS tissues and cell lines and established its correlation with OS tumor progression and metastasis. Importantly, the prognostic significance of LINC00324 was identified in patients with OS. Gain‐ and loss‐of‐function assays revealed that LINC00324 accelerated cell proliferation and migration in OS. Mechanistically, we revealed that LINC00324 stabilized WD repeat‐containing protein 66 (WDR66) messenger RNA through interacting with Hu antigen R. Rescue assays verified that WDR66 was required for the regulation of LINC00324 in promoting proliferation and migration of OS cells. In conclusion, the present study proved that LINC00324 accelerated the proliferation and migration of osteosarcoma cells through regulating WDR66, providing a new prognostic target for osteosarcoma.
Weighted gene correlation network analysis identifies RSAD2, HERC5, and CCL8 as prognostic candidates for breast cancer
Authors: Tang Jianing , Yang Qian , Cui Qiuxia , Zhang Dan , Kong Deguang , Liao Xing , Ren Jiangbo , Gong Yan , Wu Gaosong 
Published: June 2019

AbstractAs the most commonly diagnosed malignant tumor in female population, the prognosis of breast cancer is affected by complex gene interaction networks. In this research weighted gene co‐expression network analysis (WGCNA) would be utilized to build a gene co‐expression network to identify potential biomarkers for prediction the prognosis of patients with breast cancer. We downloaded GSE25065 from Gene Expression Omnibus database as the test set. GSE25055 and GSE42568 were utilized to validate findings in the research. Seven modules were established in the GSE25065 by utilizing average link hierarchical clustering. Three hub genes, RSAD2, HERC5, and CCL8 were screened out from the significant module (R
2 = 0.44), which were considerably interrelated to worse prognosis. Within test dataset GSE25065, RSAD2, and CCL8 were correlated with tumor stage, grade, and lymph node metastases, whereas HERC5 was correlated with lymph node metastases and tumor grade. In the validation dataset GSE25055 and RSAD2 expression was correlated with tumor grade, stage, and size, whereas HERC5 was related to tumor stage and tumor grade, and CCL8 was associated with tumor size and tumor grade. Multivariable survival analysis demonstrated that RSAD2, HERC5, and CCL8 were independent risk factors. In conclusion, the WGCNA analysis conducted in this study screened out novel prognostic biomarkers of breast cancer. Meanwhile, further in vivo and in vitro studies are required to make the clear molecular mechanisms.
Cellular study of the LPS‐induced osteoclastic multinucleated cell formation from RAW264.7 cells
Authors: Xiao Yuan , Cao Yang , Song Chengchao , Ren Xiaoyu , Yan Liang , Hao Dingjun , Kong Lingbo 
Published: June 2019

AbstractDespite the response to the receptor activator of nuclear factor κ‐Β ligand (RANKL), a study has reported that lipopolysaccharide (LPS) could induce RAW264.7 linage osteoclastic differentiation. However, on the contrary, another study recently showed that the LPS‐induced multinuclear cells from RAW264.7 did not express osteoclastic functions. Interestingly, in our previous study, we found that RAW264.7 cells pretreated with 10 ng LPS plus macrophage‐colony stimulating factor did not show any effects for enhancing RANKL‐induced osteoclastic cell differentiation. Therefore, in our current study, we aim to investigate the oteoclastogesis induction ability and efficacy of LPS in the RAW264.7 cell line and relevant molecular signaling. The osteoclastogenic activity of LPS‐treated RAW264.7 linage was studied by bone resorption pits and fibrous actin study. Besides that, through polymerase chain reaction and western blot, we showed that the transcriptional factor c‐Fos and Nfatc1 might be associated with LPS‐induced osteoclastogenesis. Overall, the results of our current study showed positive proof for osteoclast generation from LPS‐independent treatment, as well as established an optimal and efficient method for this process.
Solution Properties and Displacement Characteristics of in situ CO2 Foam System for Enhanced Oil Recovery
Authors: Jin Fa‐yang , Du Daijun , Pu Wanfen , Wang Song 
Published: June 2019

AbstractCarbon dioxide (CO2) foam flooding has been shown to enhance oil recovery. However, large‐scale adoption has been restricted by issues with transportation of CO2 and equipment corrosion. In situ CO2 foam generation can possibly overcome these issues. In this article, a CO2 sustained‐release system was first optimized for the CO2 production rate and production efficiency. Then, the dissolution capacity and plug‐removing ability of the sustained‐release system were evaluated. Visual experiment and parallel sand pack flooding tests were conducted to verify the formation, propagation of in situ CO2 foam, and the feasibility of this technique. The results indicated that the sustained‐release system had benign ability to lower injection pressure and improve injectability. Moreover, in situ CO2 foam flooding could obtain high oil recovery due to favorable mobility control ability, interfacial tension reduction capacity, and heterogeneity improvement. All the experiments demonstrated that the in situ CO2 foam technique has great potential for enhanced oil recovery in the Bohai oilfield.
Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis
Authors: de la Luz Cádiz‐Gurrea María , Zengin Gokhan , Kayacık Ozlem , Lobine Devina , Mahomoodally Mohamad Fawzi , Leyva‐Jiménez Francisco Javier , Segura‐Carretero Antonio 
Published: June 2019

AbstractBACKGROUNDIn this study, we aimed to evaluate the influence of different extraction procedures [decoction, homogenizer‐assisted extraction (HAE), infusion, maceration, Soxhlet and ultrasound‐assisted extraction (UAE)] on the chemical profiling and biological properties of methanol and water extracts of Pulicaria dysenterica (L.) Bernh. The chemical profiles of the extracts were evaluated by high‐performance liquid chromatography coupled to electrospray ionization and time‐of‐flight mass spectrometry (HPLC‐ESI‐TOF‐MS). The antioxidant properties and enzymes (lipase, α‐amylase, α‐glucosidase, tyrosinase and cholinesterases) inhibitory potential of the extracts were evaluated.RESULTSThe chemical profiles were dependent on the type of extraction methods as well as on the type of solvent. The methanolic extracts showed higher levels of total phenolic, flavonoid, and phenolic acid content, while the highest total flavonol content was observed in the HAE–water extract. Forty different compounds were identified from P. dysenterica. In relation to the potential in vitro anti‐diabetic effects, the highest activity against the studied key enzymes was observed for the macerated extract (α‐amylase: 0.58 ± 0.03 and α‐ glucosidase: 1.65 ± 0.03 mmol ACAE g−1). The HAE–methanol extract was the most potent inhibitor of cholisterases, whereas the highest activities against tyrosinase were observed for UAE–methanol extract, followed by macerated and Soxhlet. The inhibitory activity of the studied extracts against lipase were in the order: soxhlet > macerated> HAE–methanol > UAE–methanol.CONCLUSIONThis study has established scientific baseline data on the therapeutic properties of P. dysentrica, thereby advocating the need for further investigations in an endeavour to develop novel pharmaceuticals from this plant. © 2019 Society of Chemical Industry
Estimating abundances, densities, and interspecific associations in a carnivore community
Authors: Forsyth David M. , Ramsey David S. L. , Woodford Luke P. 
Published: June 2019

ABSTRACTEstimating population abundances, densities, and interspecific interactions are common goals in wildlife management. Camera traps have been used to estimate the abundance and density of a single species, and are useful for carnivores that occur at low densities. Spatial capture–recapture (SCR) models can be used to estimate abundance and density from a camera trap array when all, some, or no individuals in the population can be uniquely identified. These SCR models also estimate locations of individual activity centers, the spatial patterning of which could provide important information about interspecific interactions. We used SCR models to estimate abundances, densities, and activity centers of each of 3 carnivore species (i.e., dingo [Canis familiaris], red fox [Vulpes vulpes], and feral cat) using photographs from 1 camera trap array in southeastern Australia during September to November 2015. Some dingoes and feral cats were uniquely identifiable and therefore, we used a spatial mark–resight model for these species. We could not uniquely identify fox individuals, however, so we used a spatial unmarked (SUN) model for this species. Our estimated dingo density was 0.06/km2. The fox (0.25/km2) and feral cat (0.16/km2) densities are within the ranges previously reported for these species in Australia. We obtained a relatively imprecise fox density estimate because we did not have detections of uniquely identifiable individuals; hence, the SUN model should be used as a last resort. We next modeled spatial dependence among the estimated activity centers for the 3 species using a spatial pair correlation function for a marked point process. Consistent with our expectations, the activity centers of dingoes and foxes were strongly negatively associated at distances of <1,000 m. Foxes and feral cats were also negatively associated at distances of <1,500 m. Surprisingly, dingoes and feral cats were positively associated at distances of >500 m, with no association evident at distances of <500 m. Our study extends the inferences that can be made from using a camera trap array and SCR methods to include spatial patterning and interspecific interactions, and provides new insights into the carnivore community of dingoes, foxes, and feral cats in southeastern Australia. © 2019 The Authors. The Journal of Wildlife Management Published by Wiley Periodicals, Inc.
Preparation of Small‐Diameter Tissue‐Engineered Vascular Grafts Electrospun from Heparin End‐Capped PCL and Evaluation in a Rabbit Carotid Artery Replacement Model
Authors: Jin Xin , Geng Xue , Jia Liujun , Xu Zeqin , Ye Lin , Gu Yongquan , Zhang Ai‐Ying , Feng Zeng‐Guo 
Published: June 2019

AbstractAiming to construct small diameter (ID <6 mm) off‐the‐shelf tissue‐engineered vascular grafts, the end‐group heparinizd poly(ε‐caprolactone) (PCL) is synthesized by a three‐step process and then electrospun into an inner layer of double‐layer vascular scaffolds (DLVSs) showing a hierarchical double distribution of nano‐ and microfibers. Afterward, PCL without the end‐group heparinization is electrospun into an outer layer. A steady release of grafted heparin and the existence of a glycocalyx structure give the grafts anticoagulation activity and the conjugation of heparin also improves hydrophilicity and accelerates degradation of the scaffolds. The DLVSs are evaluated in six rabbits via a carotid artery interpositional model for a period of three months. All the grafts are patent until explantation, and meanwhile smooth endothelialization and fine revascularization are observed in the grafts. The composition of the outer layer of scaffolds exhibits a significant effect on the aneurysm dilation after implantation. Only one aneurysm dilation is detected at two months and no calcification is formed in the follow‐up term. How to prevent aneurysms remains a challenging topic.
Correlations of quantitative MRI metrics with myelin basic protein (MBP) staining in a murine model of demyelination
Authors: Soustelle Lucas , Antal Maria C. , Lamy Julien , Rousseau François , Armspach Jean‐Paul , Loureiro de Sousa Paulo 
Published: June 2019

Myelin imaging in the central nervous system is essential for monitoring pathologies involving white matter alterations. Various quantitative MRI protocols relying on the modeling of the interactions of water protons with myelinated tissues have shown sensitivities in case of myelin disruption. Some extracted model parameters are more sensitive to demyelination, such as the bound pool fraction (f) in quantitative magnetization transfer imaging (qMTI), the radial diffusivity in diffusion tensor imaging (DTI), and the myelin water fraction (MWF) in myelin water imaging (MWI). A 3D ultrashort echo time (UTE) sequence within an appropriate water suppression condition (Diff‐UTE) is also considered for the direct visualization of the myelin semi‐solid matrix (Diff‐UTE normalized signal; rSPF). In this paper, we aimed at assessing the sensitivities and correlations of the parameters mentioned above to an immuno‐histological study of the myelin basic protein (MBP) in a murine model of demyelination at 7 T. We demonstrated a high sensitivity of the MRI metrics to demyelination, and strong Spearman correlations in the corpus callosum between histology, macromolecular proton fraction (ρ>0.87) and Diff‐UTE signal (ρ>0.76), but moderate ones with radial diffusivity and MWF (|ρ|<0.70).
Insights into pediatric rhabdomyosarcoma research: Challenges and goals
Authors: Yohe Marielle E. , Heske Christine M. , Stewart Elizabeth , Adamson Peter C. , Ahmed Nabil , Antonescu Cristina R. , Chen Eleanor , Collins Natalie , Ehrlich Alan , Galindo Rene L. , Gryder Berkley E. , Hahn Heidi , Hammond Sharon , Hatley Mark E. , Hawkins Douglas S. , Hayes Madeline N. , Hayes‐Jordan Andrea , Helman Lee J. , Hettmer Simone , Ignatius Myron S. , Keller Charles , Khan Javed , Kirsch David G. , Linardic Corinne M. , Lupo Philip J. , Rota Rossella , Shern Jack F. , Shipley Janet , Sindiri Sivasish , Tapscott Stephen J. , Vakoc Christopher R. , Wexler Leonard H. , Langenau David M. 
Published: June 2019

AbstractOverall survival rates for pediatric patients with high‐risk or relapsed rhabdomyosarcoma (RMS) have not improved significantly since the 1980s. Recent studies have identified a number of targetable vulnerabilities in RMS, but these discoveries have infrequently translated into clinical trials. We propose streamlining the process by which agents are selected for clinical evaluation in RMS. We believe that strong consideration should be given to the development of combination therapies that add biologically targeted agents to conventional cytotoxic drugs. One example of this type of combination is the addition of the WEE1 inhibitor AZD1775 to the conventional cytotoxic chemotherapeutics, vincristine and irinotecan.