bluuebunny/crossref_metadata_2025_split huggingface dataset:
Centrin2 from the human parasite Toxoplasma gondii is required for its invasion and intracellular replication
Authors: Leung Jacqueline M. , Liu Jun , Wetzel Laura A. , Hu Ke 
Published: June 2019

ABSTRACT
Centrins are EF-hand containing proteins ubiquitously found in eukaryotes and are key components of centrioles/basal bodies as well as certain contractile fibers. We previously identified three centrins in the human parasite Toxoplasma gondii, all of which localized to the centrioles. However, one of them, T. gondii (Tg) Centrin2 (CEN2), is also targeted to structures at the apical and basal ends of the parasite, as well as to annuli at the base of the apical cap of the membrane cortex. The role(s) that CEN2 play in these locations were unknown. Here, we report the functional characterization of CEN2 using a conditional knockdown method that combines transcriptional and protein stability control. The knockdown resulted in an ordered loss of CEN2 from its four compartments, due to differences in incorporation kinetics and structural inheritance over successive generations. This was correlated with a major invasion deficiency at early stages of CEN2 knockdown, and replication defects at later stages. These results indicate that CEN2 is incorporated into multiple cytoskeletal structures to serve distinct functions that are required for parasite survival.
Does evidence support the high expectations placed in precision medicine? A bibliographic review
Authors: Cortés Jordi , González José Antonio , Medina María Nuncia , Vogler Markus , Vilaró Marta , Elmore Matt , Senn Stephen John , Campbell Michael , Cobo Erik 
Published: June 2019

Background: Precision medicine is the Holy Grail of interventions that are tailored to a patient’s individual characteristics. However, conventional clinical trials are designed to find differences in averages, and interpreting these differences depends on untestable assumptions. Although only an ideal, a constant effect of treatment would facilitate individual management. A direct consequence of a constant effect is that the variance of the outcome measure would be the same in the treated and control arms. We reviewed the literature to explore the similarity of these variances as a foundation for examining whether and how often precision medicine is definitively required. Methods: We reviewed parallel clinical trials with numerical primary endpoints published in 2004, 2007, 2010 and 2013. We collected the baseline and final standard deviations of the main outcome measure. We assessed homoscedasticity by comparing the variance of the primary endpoint between arms through the outcome variance ratio (treated to control group). Results: The review provided 208 articles with enough information to conduct the analysis. One out of five studies (n = 40, 19.2%) had statistically different variances between groups, implying a non-constant-effect. The adjusted point estimate of the mean outcome variance ratio (treated to control group) is 0.89 (95% CI 0.81 to 0.97). Conclusions: The mean variance ratio is significantly lower than 1 and the lower variance was found more often in the intervention group than in the control group, suggesting it is more usual for treated patients to be stable. This observed reduction in variance might also imply that there could be a subgroup of less ill patients who derive no benefit from treatment. This would require further study as to whether the treatment effect outweighs the side effects as well as the economic costs. We have shown that there are ways to analyze the apparently unobservable constant effect.
de.NBI Cloud federation through ELIXIR AAI
Authors: Belmann Peter , Fischer Björn , Krüger Jan , Procházka Michal , Rasche Helena , Prinz Manuel , Hanussek Maximilian , Lang Martin , Bartusch Felix , Gläßle Benjamin , Krüger Jens , Pühler Alfred , Sczyrba Alexander 
Published: June 2019

The academic de.NBI Cloud offers compute resources for life science research in Germany. At the beginning of 2017, de.NBI Cloud started to implement a federated cloud consisting of five compute centers, with the aim of acting as one resource to their users. A federated cloud introduces multiple challenges, such as a central access and project management point, a unified account across all cloud sites and an interchangeable project setup across the federation. In order to implement the federation concept, de.NBI Cloud integrated with the ELIXIR authentication and authorization infrastructure system (ELIXIR AAI) and in particular Perun, the identity and access management system of ELIXIR. The integration solves the mentioned challenges and represents a backbone, connecting five compute centers which are based on OpenStack and a web portal for accessing the federation.This article explains the steps taken and software components implemented for setting up a federated cloud based on the collaboration between de.NBI Cloud and ELIXIR AAI. Furthermore, the setup and components that are described are generic and can therefore be used for other upcoming or existing federated OpenStack clouds in Europe.
Successful Treatment of Hereditary Hemorrhagic Telangiectasia With Octreotide
Authors: Houghton Kimberly D. , Umar Beena , Schairer Jason 
Published: June 2019

ABSTRACT
Hereditary hemorrhagic telangiectasia (HHT) is a disorder characterized by telangiectasias and arteriovenous malformations. We present a case report of a 74-year-old man diagnosed with HHT having a favorable response to a somatostatin analogue for treatment. This patient had been suffering from chronic anemia from recurrent gastrointestinal bleeding, requiring oral/intravenous iron replacement, frequent endoscopic ablations, and blood transfusions. Due to insufficient treatment, he was started on subcutaneous octreotide, with significant improvement as evidenced by a steady increase in the hemoglobin level, decreased endoscopic interventions, and decreased blood transfusions, making this the first case of HHT successfully treated with octreotide.
EXPERIENCIAS ESTUDIANTILES Y DIFICULTADES MÁS ACUCIANTES EN EL INGRESO UNIVERSITARIO.
Authors: Fascendini Paola Yanina 
Published: June 2019

Son numerosas las referencias que evidencian las dificultades y obstáculos que los estudiantes manifiestan al momento del ingreso, como así también los motivos por los cuales, algunos de ellos, abandonan sus estudios.Los objetivos de este trabajo son visibilizar las problemáticas vivenciadas por los estudiantes en relación con las habilidades y estrategias para aprender, como así también conocer las experiencias acaecidas en la escuela secundaria y el inicio en esta formación profesional. Los resultados obtenidos nos indican que los estudiantes no cuentan con el bagaje instrumental necesario para hacer frente a los desafíos que implica una educación superior, viéndose obnubilados por las demandas académicas existentes, como así también, por la pérdida de referentes anteriores, que implican el desafío de volver a construir el oficio de estudiante. Otros aspectos dignos de destacar, son las dificultades orales que refieren a la hora de expresarse y las interferencias acontecidas en la construcción de vínculos interpersonales que tienen que generar ante un nuevo mundo de coetáneos (factor psicosocial). El aporte y beneficio de la investigación radica, en propiciar espacios y oportunidades a los estudiantes donde no sólo se conozcan a sí mismos sino también puedan construir las herramientas faltantes, tan reclamadas y poco trabajadas.
Efek Dexmedetomidine 0,2 ug/kgbb Intravena terhadap Insiden Delirium saat Pulih Sadar dari Anestesi Umum pada Pasien Pediatrik
Authors: Hendrawan Cahya , Arif Syafri Kamsul 
Published: June 2019

Latar Belakang : Dexmedetomidine memberikan efek sedasi, analgesia, dan anxiolitiksetelah pemberian intravena. Isofluran dan sevofluran dihubungkan dengan angkakejadian delirium saat pulih sadar dari anestesi umum pada pasien pediatrik. Padapenelitian dengan menggunakan placebo sebagai kontrol, kami mengevaluasi efek daridosis tunggal dexmedetomidine pada delirium saat pulih sadar dari anestesi umumpada pasien pediatrik yang menjalani pembedahan elektif menggunakan anestesiumum dengan isofluran.Metode : Pada penelitian acak tersamar ganda ini , 46 anak (usia 3-10 tahun) dipilihsecara acak mendapatkan dexmedetomidin 0,2ug/kgBB atau placebo pada akhirpembedahan. Semua pasien mendapatkan obat anestesi yang standar. Setelahpembedahan, nilai delirium saat pulih sadar dari anestesi umum diukur sampai 1 jampascabedah. Waktu ekstubasi, waktu pulih sadar, dan efek samping daridexmedetomidine dicatat. Setelah pembedahan nyeri pasien diukur denganmenggunakan objective pain scale (OPS) .Hasil : Nilai delirium saat pulih sadar dari anestesi umum pada kelompokdexmedetomidine lebih baik daripada kelompok placebo (P<0,05). Nilai nyeri samapada kedua kelompok (P>0,05). Waktu ekstubasi dan waktu pulih sadar lebih panjangpada kelompok dexmedetomidin tetapi tidak bermakna secara statistik (P>0,05). Tidakada efek samping (hipotensi dan bradikardi) pada kedua kelompok.Kesimpulan : Kami menyimpulkan bahwa Dexmedetomidine 0,2ug/kgBB intravenadapat mengurangi insiden delirium saat pulih sadar dari anestesi umum denganisofluran pada anak yang menjalani pembedahan elektif.
Perbandingan Efektivitas Spray Mometasone Furoat dan Deksamethason Intravena dalam Mengurangi Nyeri Tenggorokan setelah Operasi pada Anestesi Umum Intubasi Endotrakeal
Authors: Wirdiyana Dian , Wahyudi Wahyudi , Achmad Muh Ramli 
Published: June 2019

Latar belakang : Banyak faktor pada intubasi endotrakeal yang dapat menyebabkan nyeri tenggorokan setelah operasi sebagai akibat dari trauma pada mukosa. Spray mometasone furoat adalah kortikosteroid potensi sedang yang dapat mencegah influx sel-sel inflamasi ke dalam mukosa. Deksamethason adalah kortikosteroid potensi kuat dengan sifat analgetik dan antiinflamasi.Tujuan: meneliti perbandingan efektivitas spray mometasone furoat dan deksamethason intravena dalam mengurangi nyeri tenggorokan setelah operasi pada anestesi umum intubasi endotrakeal. Metode : Penelitian ini bersifat double blind. Lima puluh delapan pasien yang akan menjalani operasi dengan anestesi umum intubasi endotrakeal dipilih secara acak. Sampel yang memenuhi kriteria inklusi di bagi dalam 2 grup. Grup M (n = 29) diberikan spray mometasone furoat 100 mcg pada cuff pipa endotrakeal, pita suara, epiglottis, dan faring saat intubasi endotrakel dan grup D (n = 29) diberikan deksamethason 10 mg intravena 30 menit sebelum intubasi endotrakeal. Dilakukan penilaian terhadap insiden dan derajat nyeri tenggorokan pada jam ke-1, ke-6, dan ke- 24 setelah ektubasi. Analisis statistik dilakukan dengan uji mann-whitney dan chi square, dengan p < 0,05 bermakna secara signifikan. Hasil : Terdapat perbedaan yang bermakna baik insiden maupun derajat nyeri tenggorokan pada jam ke-1 setelah ekstubasi pada kedua kelompok (p<0,05). Tidak terdapat perbedaan bermakna baik insiden maupun derajat nyeri tenggorokan pada jam ke-6 dan ke-24 setelah ekstubasi pada kedua kelompok (p>0,05). Kesimpulan : Spray mometasone furoat dapat mengurangi nyeri tenggorokan setelah operasi anestesi umum intubasi endotrakeal.
Efektivitas Midazolam Untuk Pencegahan Mual Muntah Pascabedah Pada Prosedur Laparaskopi
Authors: Jamiludin Jamiludin , AAA Husain 
Published: June 2019

Latar belakang : Seluruh pasien yang menjalani pembedahan beresiko untuk mengalami mual dan muntah pasca bedah (PONV). Kejadian PONV menjadi gejala yang sangat merugikan terutama setelah prosedur pembedahan ambulatori serta mengganggu proses pemulihan pasca anestesi dan pembedahan sehingga memperpanjang waktu perawatan. Laparoskopi adalah suatu prosedur pembedahan minimal invasif yang disertai insidens PONV cukup tinggi. Penyebab tingginya angka kejadian PONV pada pembedahan laparaskopi disebabkan oleh gas yang digunakan untuk insuflasi dan menyebabkan penekanan pada nervus vagus yang memiliki hubungan dengan pusat muntah di medulla oblongata. Selain itu, penyebab lain seperti teknik anestesi, jenis kelamin, nyeri, perawatan pasca operatif dan data demografik pasien yang berhubungan dengan pengaruh terjadinya emesis. Midazolam sebagai agen anti emetik dan anxiolitik yang menurunkan sintesis, pelepasan dan efek pasca sinaptik dopamin serta menhambat reuptake adenosin, sehingga menurunkan input dopamin dan 5-HT3 terhadap CRTZ dan mengurangi input dari thalamus yang mempengaruhi langsung pusat muntah.Tujuan: mengevaluasi pemberian midazolam sebagai agen anti emetik dan anxiolitikMetode: Empat puluh delapan pasien yang akan menjalani prosedur pembedahan laparaskopi elektif secara acak dibagi menjadi dua kelompok. Setelah diberikan obat premedikasi, kelompok M (n=24) diberikan midazolam 35 μg/kgBB intravena kelompok O (n=24) diberikan ondansetron 4 mg intravena. Selama prosedur anestesi, pemakaian opioid dan lama operasi dicatat. Kemudian kejadian mual muntah pasca bedah diamati dan dicatat selama periode 8 jam pascabedah.Hasil: Kejadian mual muntah setelah prosedur pembedahan laparaskopi pada penelitian ini diukur menggunakan skor PONV dengan interval 30 menit selama di ruang pemulihan dan setiap 1 jam di ruang perawatan selama 8 jam pasca bedah. Pada penilitian ini, terdapat perbedaan yang bermakna diantara kedua kelompok dengan hasil p=0,022 (p<0,05) pada waktu pengamatan P2 (60 menit pasca bedah) .Kesimpulan: Midazolam 35 μg/kgBB setelah premedikasi pada anestesi umum pada prosedur pembedahan laparaskopi elektif menurunkan kejadian mual muntah pascabedah terutama pada 1 jam pasca bedah.
O PAPEL DE UMA FARMÁCIA UNIVERSITÁRIA NO TRATAMENTO DE FERIDAS CRÔNICAS
Authors: Vianna Raihane Pombo , Barbosa Juliana Ricardo , Penetra Maria Alice , De Freitas Zaida Maria Faria , Dos Santos Elisabete Pereira , Di Piero Karina Chamma , Monteiro Mariana Sato de S. B. 
Published: June 2019

O objetivo desse trabalho consistiu em identificar as principais preparações de uso tópico utilizadas no tratamento de feridas, com foco nas formulações manipuladas, e descrever as que já fazem parte do elenco de medicamentos disponibilizados pela Farmácia Universitária (FU), da Universidade Federal do Rio de Janeiro (UFRJ) à população. Esse trabalho utilizou uma revisão bibliográfica narrativa, desenvolvida pela avaliação de trabalhos selecionados em artigos científicos, a partir de critérios de inclusão e exclusão, complementado com um levantamento sobre as formulações de uso tópico preparadas pela FU/UFRJ. Dentre as opções terapêuticas citadas nos diferentes artigos e protocolos algumas formulações são passíveis de manipulação por farmácias magistrais, como os hidrocolóides, hidrogéis e sulfadiazina de prata. A FU/UFRJ manipula gel de carboximetilcelulose, papaína e sulfadiazina de prata. As farmácias magistrais podem contribuir no tratamento de feridas ofertando formulações com diferentes concentrações dos ativos, que melhor se adaptem ao paciente, aumentando as possibilidades terapêuticas.
Palavras-chave: Feridas. Tratamento. Farmácia Universitária.
Incidence of Herpes Zoster Among Children: 2003–2014
Authors: Weinmann Sheila , Naleway Allison L. , Koppolu Padma , Baxter Roger , Belongia Edward A. , Hambidge Simon J. , Irving Stephanie A. , Jackson Michael L. , Klein Nicola P. , Lewin Bruno , Liles Elizabeth , Marin Mona , Smith Ning , Weintraub Eric , Chun Colleen 
Published: June 2019

BACKGROUND AND OBJECTIVES:
After the 1996 introduction of routine varicella vaccination in the United States, most studies evaluating pediatric herpes zoster (HZ) incidence reported lower incidence over time, with varying degrees of decline. Using the combined databases of 6 integrated health care organizations, we examined HZ incidence in children over a 12-year period in the varicella vaccine era.

METHODS:
This study included children aged 0 through 17 years from 2003 through 2014. Using electronic medical records, we identified HZ cases through International Classification of Diseases, Ninth Revision diagnosis code 053. We calculated HZ incidence rates per 100 000 person years of health plan membership for all children and among children who were vaccinated versus unvaccinated. We calculated rates for the 12-year period and examined temporal trends. Among children who were vaccinated, we compared HZ rates by month and year of age at vaccination.

RESULTS:
The study included 6 372 067 children with ≥1 month of health plan membership. For the 12-year period, the crude HZ incidence rate for all subjects was 74 per 100 000 person years, and the rate among children who were vaccinated was 38 per 100 000 person years, which was 78% lower than that among children who were unvaccinated (170 per 100 000 person years; P &lt; .0001). Overall HZ incidence declined by 72% (P &lt; .0001) from 2003 through 2014. Annual rates in children who were vaccinated were consistently lower than in children who were unvaccinated.

CONCLUSIONS:
With this population-based study, we confirm the decline in pediatric HZ incidence and the significantly lower incidence among children who are vaccinated, reinforcing the benefit of routine varicella vaccination to prevent pediatric HZ.
Метод розрахунку теплообміну під час конденсації холодоагентів у середині горизонтальних труб у разі стратифікованого режиму течії фаз
Authors: Горін В. В. , Середа В. В. , Барабаш П. О. 
Published: June 2019

У сучасних конденсаторах систем кондиціонування повітря, теплових насосів, випарниках систем опріснювання морської води і нагрівачах електростанцій процес конденсації пари здійснюється переважно у середині горизонтальних труб і каналів. Процеси теплообміну, що відбуваються у теплообмінниках цього типу, мають суттєвий вплив на загальну енергоефективність таких систем. У даній роботі представлено експериментальні дослідження теплообміну у разі конденсації холодоагентів R22, R406A, R407C у гладкій горизонтальній трубі з внутрішнім діаметром d = 17 мм за наступними режимними параметрами:температура насичення 35 - 40ºC, масова швидкість 10 - 100 кг/кв.м/c, масовий паровміст 0,1 - 0,8, питомий тепловий потік 5 ‑ 50 кВт/кв.м, різниця між температурою конденсації та температурою стінки труби 4 - 14 К. Вимірювання локальних за перерізом труби теплових потоків і коефіцієнтів тепловіддачі проводились за методом «товстої стінки» під час різних режимів конденсації. За результатами досліджень установлено, що у верхній частині труби з підвищенням теплового потоку зростає товщина плівки конденсату, що призводить до зменшення тепловіддачі. У нижній частині труби збільшення теплового потоку підвищує тепловіддачу, що характерно для турбулентної течії рідини в трубі. Отримані результати роботи дозволили покращити метод розрахунку теплообміну у разі конденсації пари, яка ураховує вплив течії конденсату у нижній частині труби на теплообмін. Цей метод із достатньою точністю (похибка ±30%) узагальнює експериментальні дані під час конденсації пари холодоагентів R22, R134a, R123, R125, R32, R410a за умови стратифікованого потоку. Використання цього методу у разі проектування теплообмінних апаратів, які використовують такі типи речовин, підвищить ефективність енергетичних систем.
ÖRGÜTSEL YABANCILAŞMA VE İÇ GİRİŞİMCİLİK ARASINDAKİ İLİŞKİDE PSİKOLOJİK RAHATLIK ALGISININ DÜZENLEYİCİ ROLÜ
Authors: Yener Serdar 
Published: June 2019

Bu çalışmada örgütlerde son yıllarda farklı
nedenlerden dolayı yaşanan örgütsel yabancılaşma duygusunun örgütlerin
sürdürülebilir rekabet avantajı (SRA) sürecindeki belirleyenlerinden iç
girişimcilik davranışı üzerine olan etkisi sorgulanmıştır. Ayrıca bu etkide
psikolojik rahatlık algısının düzenleyici rolü sorgulanmıştır. İç girişimcilik
davranışı çalışanların örgüt içinde verim ve etkinliği arttırmak için
gösterdikleri yenilik ve yaratıcılık güdümlü davranışları ifade etmektedir.
Örgütsel yabancılaşma örgütte çalışanların güçsüzlük, çaresizlik, umutsuzluk, anlamsızlık duygularını ifade
etmek için kullanılan olumsuz bir duygudur. Psikolojik rahatlık algısı
çalışanların örgütte hissetmiş oldukları kişilerarası saygı ve hoşgörü
seviyesidir. Sinop il ve ilçelerinde bulunan KOBİ’lerde çalışan 159 katılımcıya
uygulanan anket sonuçlarına göre psikolojik rahatlık algısının düzenleyici rolü
gözlenmiştir. Normlara uymama kuramı ve kolektivist grup içinde bireysel
davranışlarla psikolojik rahatlık algısının örgütsel yabancılaşma ve iç
girişimcilik arasındaki ilişkideki düzenleyici etkisi açıklanmaya
çalışılmıştır.
Work-Based Learning Experiences for Students With Disabilities: Ensuring Compliance With the Fair Labor Standards Act
Authors: Riesen Tim , Thomas Faith , Kipping Kayla Currier 
Published: June 2019

BackgroundWork-based learning strategies are often used in secondary transition settings to identify a student's strengths and interest related to competitive integrated employment. Transition professional must not only understand evidence-based practices for creating and supporting work-based learning, they must also understand specific statutory provisions outlined in the Fair Labor Standards Act (FLSA).ObjectiveThis article provides a targeted review of the FLSA to highlight specific provisions that transition professionals must understand when facilitating work-based learning activities in secondary transition settings.ConclusionsThe article will conclude with specific recommendations about how to implement and supervise work-based learning in compliance with the FLSA.
Does public procurement for innovation increase innovative efforts? The case of Brazil
Authors: Rocha Frederico 
Published: June 2019

The paper uses microdata from the Brazilian Innovation Survey to assess the effect of public procurement on firms’ private R&D expenditures. The PINTEC 2014 edition included in its policy module a new question about public procurement related to firms’ innovative activities. The paper uses this information to define a treated sample composed by firms that participated in public procurement with innovative attributes. The paper builds a control sample through coarsened exact matching and tests the effect of innovation-related public procurement on firms’ private R&D to sales ratio. The results suggest a positive impact of public procurement of innovation on R&D intensity. Data also shows that most firms involved in PPI are small and belong to low-technology sectors. These features disclose an important characteristic of the public procurement of innovation: its ability to target small firms and contribute to local development.
Energy efficiency improvement in the system for drying baker`s yeast
Authors: Anastasovski Aleksandar Kosta 
Published: June 2019

Drying processes are one of the main consumers of heat energy in production. Any decreases in heat consumption during the drying process will considerably decrease production costs. This study analyzes the high consumption of heat in the drying of baker`s yeast. The main task is to minimize the energy demand and lower the price of the final products with partial heat recovery. These changes will require system modifications. One of the most popular and effective methods that can be used in this case is heat process integration with Pinch Technology. In this study, a reference system was simulated with a mathematical model and analyzed for waste heat streams.This paper suggests the redesigning of a drying system for production of active dry yeast. Selected streams that satisfy conditions for heat process integration were involved in the evaluation for a better solution. Two different scenarios were proposed as possible solutions. The suggested solutions are retrofit designs of Heat Exchanger Networks. These Heat Exchanger Networks include already installed heat exchangers as well as new heat transfer units. The selection of better design was made with economic analysis of investment. The proposed scenarios of the analyzed sub-system give improvement in heat energy recovery. The best determined solution reduces the cost and thus has the highest profitability, but not the highest heat energy recovery.