bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR‐96‐5p regulates wound healing by targeting BNIP3/FAK pathway
Authors: Wu Peng , Cao Yongqian , Zhao Ran , Wang Yibing 
Published: March 2019

AbstractCutaneous wound healing is a highly orchestrated basic biological process and one of the key processes in restoring skin integrity. The role of microRNAs (miRNAs) during this process has raised numerous attention and is poorly explored. The aim of this study is to investigate the potential function of BCL2 interacting protein (BNIP3) and its target miRNA, miR‐96‐5p, in cutaneous wound healing. The results demonstrated that BNIP3 was significantly increased and miR‐96‐5p was obviously decreased during wound healing. Overexpression of BNIP3 significantly increased, while inhibition of BNIP3 decreased cell proliferation and migration of human primary keratinocytes. miR‐96‐5p was predicted to be a target miRNA for BNIP3 and luciferase reporter assay confirmed that miR‐96‐5p directly targeted the 3′‐untranslated region of BNIP3. Moreover, miR‐96‐5p overexpression significantly decreased, while miR‐96‐5p inhibition dramatically increased BNIP3 protein expression and focal adhesion kinase (FAK) pathway activation. Furthermore, miR‐96‐5p inhibited cell proliferation and migration of human primary keratinocytes. Overall, our findings suggest that miR‐96‐5p might be critical in the regulation of wound healing by mediating BNIP3 and FAK pathway.
The potential value of red blood cell distribution width in patients with invasive hydatidiform mole
Authors: Zhang Lingling , Xie Youjun , Zhan Lingling 
Published: March 2019

BackgroundRed blood cell distribution width (RDW) has attracted increasing attention in cancer. The aim of this study was to assess the changes of RDW in patients with invasive hydatidiform mole and analyze the relationship between RDW and invasive hydatidiform mole.MethodsA retrospective analysis was performed on 102 patients diagnosed as invasive hydatidiform mole in the First Affiliated Hospital of Guangxi Medical University from January 2009 to March 2018. A total of 120 healthy subjects were used as a control group. The Mann‐Whitney U test was used for comparison between the invasive hydatidiform mole and control groups. Comparison of RDW with other blood parameters was performed using Spearman's. The area under the ROC curve (AUC) and 95% confidence interval (95% CI) were also determined.ResultsThe RDW, platelet‐lymphocyte ratio (PLR), neutrophil‐lymphocyte ratio (NLR), and absolute lymphocyte count were significantly elevated in the invasive hydatidiform mole group compared with control group. The hemoglobin (Hb) concentration, mean red blood cell volume (MCV) and platelet count (PLT) were significantly lower in invasive hydatidiform mole group than control group. Grade III and above invasive hydatidiform mole patients had higher levels of RDW than grade I and II patients. Correlation analysis showed that RDW was negatively correlated with Hb, MCV, NLR, and neutrophil count, but positively correlated with PDW and different stages of invasive hydatidiform mole. The ROC curve showed that the AUC of the RDW was 0.660 (95% CI 0.581‐0.740; P < 0.01).ConclusionThis study reveals the potential value of RDW in invasive hydatidiform mole.
Higher education, professional occupation, and upper socioeconomic status are associated with lower adherence to medications in patients with inflammatory bowel disease
Authors: Tomar Sanjeevani K , Kedia Saurabh , Singh Namrata , Upadhyay Ashish D , Kamat Nagesh , Bopanna Sawan , Yadav Dawesh P , Goyal Sandeep , Jain Saransh , Makharia Govind , Ahuja Vineet 
Published: March 2019

Background and AimThe literature on medication adherence in patients with inflammatory bowel disease (IBD) is heterogeneous. The present study aimed to identify the rates and predictors of nonadherence to medications in IBD.MethodsThis cross‐sectional study included patients of IBD (ulcerative colitis [UC] and Crohn's disease [CD]) recruited between November 2016 and March 2017. Adherence was assessed with a questionnaire (interview based) that evaluated patients' sociodemographic and disease profile and rates and predictors of medication adherence.ResultsA total of 266 patients (204 UC, 62 CD) were included (mean age: 38.5 ± 12.7 years, males: 142 [53.4%], mean disease duration: 6.4 ± 5.2 years). The overall adherence rate was 82.3%, with the lowest for topical therapy (67.3%) and the highest for steroids (95.9%). Predominant reasons for nonadherence were forgetting dose (18.8%), unavailability of medications (13.2%), felt better (11.7%), adverse effects (6.8%), and cost of treatment (6.0%). Patients' education (P < 0.001), occupation (P = 0.097), and socioeconomic status (P = 0.021) had a negative association with adherence. Patients in upper socioeconomic strata with professional education/occupation were the least adherent (47%), whereas patients from lower socioeconomic strata who were illiterate and unemployed had the highest adherence (100%).ConclusionMore than 80% of patients were adherent to their medications; adherence was the lowest for topical therapy. Higher education, occupation, and upper socioeconomic status were negatively associated with adherence.
Phosphatase inhibitor PPP1R11 modulates resistance of human T cells toward Treg-mediated suppression of cytokine expression
Authors: Joshi Rubin N , Fernandes Sunjay Jude , Shang Ming-Mei , Kiani Narsis A , Gomez-Cabrero David , Tegnér Jesper , Schmidt Angelika 
Published: March 2019

Abstract
Regulatory T cells (Tregs) act as indispensable unit for maintaining peripheral immune tolerance mainly by regulating effector T cells. T cells resistant to suppression by Tregs pose therapeutic challenges in the treatment of autoimmune diseases, while augmenting susceptibility to suppression may be desirable for cancer therapy. To understand the cell intrinsic signals in T cells during suppression by Tregs, we have previously performed a global phosphoproteomic characterization. We revealed altered phosphorylation of protein phosphatase 1 regulatory subunit 11 (PPP1R11; Inhibitor-3) in conventional T cells upon suppression by Tregs. Here, we show that silencing of PPP1R11 renders T cells resistant toward Treg-mediated suppression of TCR-induced cytokine expression. Furthermore, whole-transcriptome sequencing revealed that PPP1R11 differentially regulates not only the expression of specific T cell stimulation-induced cytokines but also other molecules and pathways in T cells. We further confirmed the target of PPP1R11, PP1, to augment TCR-induced cytokine expression. In conclusion, we present PPP1R11 as a novel negative regulator of T cell activation-induced cytokine expression. Targeting PPP1R11 may have therapeutic potential to regulate the T cell activation status including modulating the susceptibility of T cells toward Treg-mediated suppression, specifically altering the stimulation-induced T cell cytokine milieu.
State‐of‐the‐art technologies in precision agriculture: a systematic review
Authors: Bhakta Ishita , Phadikar Santanu , Majumder Koushik 
Published: March 2019

AbstractBACKGROUNDThe socio‐economic status of the countries is ever‐changing due to the increasing population. ‘Green Revolution’, a paradigm shift towards technology driven agriculture, has ensured a good quality of life to some extent for this increasing population. However, it brings in several negative impacts on the environment, which poses a threat to the sustainability of agriculture and natural resources. Precision agriculture (PA) is a state‐of‐the‐art concept of site‐specific farm management that helps to overcome this threat in a smart way using modern information and communication technologies. It reduces the indecorous use of resources, pollution and hence improves quality of life, which in turn helps to achieve sustainable development goals.RESULTSThe objective of this systematic review is to understand the present status, benefits, and limitations of the state‐of‐the‐art technologies used in PA. A total 67 articles are identified following the PRISMA guideline to inspect the technical innovations at different components of PA. The articles are examined based on the novelties, measured parameters, technologies, and field of applications.CONCLUSIONThis analysis results in a significant understanding about the present knowledge gap and identification of the potential future research opportunities for sustainable agronomy. © 2019 Society of Chemical Industry
Effect of heterozygous pathogenic COL4A3 or COL4A4 variants on patients with X‐linked Alport syndrome
Authors: Zhang Yanqin , Ding Jie , Zhang Hongwen , Yao Yong , Xiao Huijie , Wang Suxia , Wang Fang 
Published: March 2019

AbstractBackgroundAlport syndrome is an inherited renal disease caused by mutations in COL4A3, COL4A4, or COL4A5 genes. Coexisting mutations in either two of the three genes in Alport patients have been reported recently. However, the effect of heterozygous mutations in COL4A3 or COL4A4 genes in X‐linked Alport syndrome (XLAS) patients is unclear.MethodsUsing targeted next‐generation sequencing, six unrelated Chinese children were identified to have a combination of a pathogenic variant in COL4A5 and a heterozygous mutation in COL4A3 or COL4A4. They were three males and three females. Another three XLAS males each with only one pathogenic variant in COL4A5 were included. The clinical data were analyzed and compared between the males in two groups (group 1, males with a pathogenic variant in COL4A5 and a heterozygous pathogenic variant in COL4A3 or COL4A4; group 2, males with only one pathogenic variant in COL4A5).ResultsPatients with XLAS who also had heterozygous pathogenic COL4A3 or COL4A4 variants accounted for 1% of Alport syndrome. In this study, three children showed coexisting pathogenic variants in COL4A5 and COL4A3. Two children showed pathogenic variants in COL4A5 and COL4A4. One child had pathogenic variants in the three COL4A3‐5 genes, in which the pathogenic variant in COL4A5 was de novo and the pathogenic variants in COL4A4 and COL4A3 were inherited independently (in trans). The site and type of mutations in COL4A5 were similar between the two groups. It was revealed that males in group 1 presented more severe proteinuria than males in group 2 (p < 0.05).ConclusionThe present study provides further evidence for complicated genotype in Alport syndrome. For the first time, we reported a case with three pathogenic variants in COL4A5, COL4A3, and COL4A4 genes. Moreover, we found that heterozygous pathogenic COL4A3 or COL4A4 variants are likely to make XLAS disease more serious.
Multiple tumors due to mosaic genome‐wide paternal uniparental disomy
Authors: Postema Floor A.M. , Bliek Jet , van Noesel Carel J.M. , van Zutven Laura J.C.M. , Oosterwijk Jan C. , Hopman Saskia M. J. , Merks Johannes H. M. , Hennekam Raoul C. 
Published: March 2019

AbstractMosaic genome‐wide paternal uniparental disomy is an infrequently described disorder in which affected individuals have signs and symptoms that may resemble Beckwith–Wiedemann syndrome. In addition, they can develop multiple benign and malignant tumors throughout life. Routine molecular diagnostics may not detect the (characteristic) low level of mosaicism, and the diagnosis is likely to be missed. Genetic counseling and a life‐long alertness for the development of tumors is indicated. We describe the long diagnostic process of a patient who already had a tumor at birth and developed multiple tumors in childhood and adulthood. Furthermore, we offer clues to recognize the entity.
Phage display derived therapeutic antibodies have enriched aliphatic content: Insights for developability issues
Authors: Kaleli Nazlı Eda , Karadag Murat , Kalyoncu Sibel 
Published: March 2019

AbstractPhage display is one of the most widely used technology for antibody discovery and engineering. Number of therapeutic antibodies derived from phage display increases rapidly due to its ease of use and ability to control antibody sequence information. Although there are numerous antibody candidates as promising therapeutics, most of them fail at later stages of development due to undesired biophysical properties. Antibody candidates with poor properties should be prevented or improved in early development phases to minimize enormous loss of time and resources. In this study, we showed that phage display derived therapeutic antibodies show higher self‐interaction and polyspecificity compared to non‐phage display derived ones. To identify molecular determinants behind this, physicochemical properties of CDR regions of 137 therapeutic antibodies were analyzed. We found multiple significant differences in both heavy and light chain CDR regions. Most profoundly, aliphatic content of HCDR3, HCDR2, and LCDR3 regions were enriched in phage display derived antibodies compared to non‐phage display derived ones. Physicochemical determinants documented here seem to play important roles in polyspecific and aggregation‐prone natures of antibodies which should be avoided in early development phases.
Does pomegranate consumption affect weight and body composition? A systematic review and meta‐analysis of randomized controlled clinical trials
Authors: Gheflati Alireza , Mohammadi Mohammad , Ramezani‐Jolfaie Nahid , Heidari Zahra , Salehi‐Abargouei Amin , Nadjarzadeh Azadeh 
Published: March 2019

Pomegranate is rich in tannins, polyphenols, flavonoids, and several other types of antioxidant compounds whose role in weight loss has been shown in previous studies. However, the results have been mixed regarding the effects of pomegranate consumption on weight loss. Electronic databases such as PubMed, Scopus, ISI Web of Science, and Google Scholar were systematically searched up to May 2018 for controlled clinical trials assessing the effect of pomegranate consumption on weight loss. A random effects model was used to calculate the weighted mean difference (WMD) and 95% confidence interval (CI) as the difference between the mean for the intervention and control groups. Thirteen randomized controlled trials (including 513 participants) were included in the present meta‐analysis. Compared with the control groups, there was no significant reduction in bodyweight, body mass index, waist circumference, and body fat percentage after pomegranate consumption (WMD = −0.23 kg, 95% CI: −0.94, 0.47, P = 0.515; WMD = −0.12 kg/m2, 95% CI: −0.29, 0.03, P = 0.132; WMD = −0.08 cm, 95% CI: −0.33, 0.17, P = 0.534; WMD = −0.05, 95% CI: −1.66, 1.55, P = 0.947, respectively). According to the results of the present systematic review and meta‐analysis, there was no significant effect of pomegranate consumption on weight and body composition indices.
Cellulose‐Based Substrate for SERS‐Promoted Histamine Picomolar Detection in Beverages
Authors: Bettini Simona , Pal Sudipto , Sawalha Shadi , Licciulli Antonio , Valli Ludovico , Giancane Gabriele , Pagano Rosanna 
Published: March 2019

AbstractHistamine is a biogenic amine (BA) playing an important physiological role in human body, even though the assumption of high histamine amount from food and beverages can cause health diseases such as, for example, cutaneous allergy, headache and nausea. In fact, histamine can be present in foods and beverages as the result of microbial metabolism induced by temperatures and storage conditions, so histamine levels are commonly used as an indicator of food safety and quality. In the present contribution, the possibility to detect low concentration of histamine by Surface Enhanced Raman Spectroscopy (SERS) by using silver nanoparticles (AgNPs)‐cellulose hybrid substrate has been proposed. An easy procedure for the fabrication of the hybrid supports was developed and SERS effect at different AgNPs amounts was tested. The developed systems allowed detecting histamine down to 10–12 M in aqueous solutions. SERS substrates have been tested directly on two different kinds of commercial white wines, the first one stored in tetra pack and the other one in glass bottles without any pre‐treatment procedure. Histamine concentrations in the real samples were obtained by recording the corresponding Raman spectra and confirmed by means of conventional HPLC analysis.
Studies towards the Synthesis of Aldgamycin – M
Authors: Muralikrishna Katta , Satyanarayana Vavilapalli , Kumar Gavireddy Chaithanya , Yadav Jhillu Singh 
Published: March 2019

AbstractA convergent and highly stereoselctive synthesis of macrolide framework of aldgamycin – M is described. The salient features of the synthesis are the utilization of enzymatic desymmetrization, Crimmin's non‐Evans syn aldol reaction, Wittig olefination, Yamaguchi esterification and Ring closing metathesis reaction (RCM).
Structure‐Based Virtual Screening and Synthesis of Mn2+ Complexes of Benzothiazole Derivatives for Designing New MRI Probes
Authors: Rosa Isael Aparecido , de Oliveira Silva Giacoppo Juliana , da Cunha Elaine F. F. , Fernandes Ítalo Antônio , de Oliveira Horvath Renato , Ionta Marisa , dos Santos Marcelo Henrique , Ramalho Teodorico C. 
Published: March 2019

AbstractBenzothiazole derivatives are highly selective and an antitumor therapeutic in vitro and in vivo, highlighting the compound 2‐ (4′‐aminophenyl) benzothiazole (ABT), which exhibits nanomolar concentration activity in vitro against some human breast cancer cells in humans. In this work, we designed new contrast agents based on the structural modification of ABT by complexing them with manganese in order to obtain paramagnetic complexes for efficient diagnosis of breast cancer. The new agents were evaluated by docking studies that allowed investigating interactions and the mode of connection between the active site of the enzyme and the compounds studied. The theoretical and experimental results both indicate that Compound 57 is a promising contrast agent.
Spectrofluorometrical Determination of Vitamin B1 in Different Matrices Using Box‐Behnken Designed Pipette Tip Solid Phase Extraction by a Carbon Nanotube Sorbent
Authors: Hashemi Sayyed Hossein , Yahyavi Hossein , Kaykhaii Massoud , Hashemi Mohammad , Mirmoghaddam Majid , Keikha Ahmad Jamali 
Published: March 2019

AbstractThis study describes development of a method for spectrofluorometrical determination of thiamine (vitamin B1) in vitamin tablets, cactus fruits, multivitamin syrups, and human plasma after its extraction by pipette tip micro‐solid phase extraction using carbon nanotube as sorbent. A response surface methodology was used for experimental design to obtain optimal response. Ten variables including pH of the sample solution, type and volume of eluent solvent, volume of sample solution, effect of ionic strength, concentration of K3[Fe(CN)6] (as derivatizing agent), concentration of NaOH, number of cycles of extraction and elution and amount of sorbent were regarded as factors in the optimization study by Box‐Behnken design. Figures of merit were calculated as: detection limit; 0.16 μg L−1, enrichment factor; 91, linear calibration curve in the range of 0.5‐130.0 μg L−1; and reproducibility (as RSD %) better than 2.4%. The method was applied to the determination of different real samples successfully.
V2O5‐Catalyzed One‐Pot Multicomponent of Pyrazol Naphthoquinone as Scaffolds for Potential Bioactive Compounds: Synthesis, Structural Study and Cytotoxic Activity
Authors: Vairaperumal Veeramani , Perumal Muthuraja , Sengodu Prakash , Shanumuganthan Saravanakumar , Paramasivam Manisankar 
Published: March 2019

AbstractIn the present investigation, we have designed synthesis of a set of potential cytotoxic agents contains pyrazoyl naphthoquinone framework, from 2‐hydroxy‐1,4‐naphthaquionone, ethylacetoacetate, substituted phenylhydrazines and aromatic aldehydes in presence of different catalysts in one‐pot four component method. Various metal triflate, lewis acids and metal oxides were tested in multi‐component reaction, among them, V2O5 acts as best catalyst. We have also optimized the synthetic conditions such as catalyst loading, solvents and reaction time to achieve maximum yield. It is noteworthy to mention here that the sustainable solvents like ethanol found to be suitable solvent. Further, the synthesized compounds have been employed for their in‐vitro anticancer activity against cervical cancer cell line (HeLa). Among the other compound, 5i has displayed highest activity in comparison with control drug (doxorubicin) with corresponding IC50 equal to 2.9 μM.
A Rechargeable Al/Graphite Battery Based on AlCl3/1‐butyl‐3‐methylimidazolium Chloride Ionic Liquid Electrolyte
Authors: Yu Zhijing , Tu Jiguo , Wang Chen , Jiao Shuqiang 
Published: March 2019

AbstractAs a new and efficient power storage device, Al‐ion batteries present good prospects due to its many advantages, such as excellent performance and rich resources. In this work, a rechargeable Al‐ion battery system has been fabricated in pouch cell based on a room temperature ionic liquid (RTIL) containing AlCl3/1‐butyl‐3‐methylimidazolium chloride (BMIC) as electrolyte. The capacity of the battery is 86.4 mAh g−1 at the 100th cycle at a current density of 50 mA g−1, and it remains above 78.0 mAh g−1 after 300 cycles. A high discharge voltage platform about 2.15 V vs. Al3+/Al is obtained. In addition, in situ X‐Ray Diffraction (XRD) measurements were conducted to confirm the intercalation/de‐intercalation mechanism in the graphite layers. All of these suggest that the Al/graphite battery based on AlCl3/BMIC electrolyte, which exhibits an excellent electrochemical performance for energy storage, may be a promising Al‐ion battery system.
Molecular‐level Understanding of the Rate‐determining Step in Esterification Reactions Catalyzed by H‐ZSM‐5 Zeolite. An Experimental and Theoretical Study
Authors: Gomes Glaucio J. , Zalazar M. Fernanda , Arroyo Pedro Augusto , Scremin Fernando. R. , Costa Michelle Budke , Bittencourt Paulo R. S. , Lindino Cleber A. , Peruchena Nélida M. 
Published: March 2019

AbstractTransformation of biomass into renewable energy products is currently one of the most promising technologies for dropping dependence on fossil fuels. Biodiesel production may improve with the use of heterogeneous catalysts, such as zeolites. In this work, computational calculations in conjunction with thermal analysis (TGA), evolved gases analysis (EGA) and IR spectroscopic studies were used in order to obtain a more detailed information on the adsorption mechanism involved in the rate‐determining step of esterification reactions inside the H‐ZSM‐5 zeolite. ATR‐FTIR spectra showed a molecular adsorption of MeOH and acetic acid (AcOH) on the H‐ZSM‐5 surface, IR spectrum of AcOH showed perturbations in the region of the bands relative to the carbonyl group C=O, indicating molecular interactions by the adsorption processes, as predicted by theoretical calculations. Adsorption of a single molecule of AcOH by C=O in a single adsorption step followed by coadsorption of MeOH is more stable than adsorption involving the zeolite proton sharing, however energies values suggested competitiveness between both mechanisms. Interactions between the adsorbed molecules and the lattice structure are crucial in controlling the adsorption observed experimentally.