bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of liraglutide on the proliferation, migration, and osteogenic differentiation of human periodontal ligament cells
Authors: Pang Yunqing , Yuan Xuemin , Guo Jia , Wang Xuemei , Yang Man , Zhu Jingli , Wang Jing 
Published: September 2018

AbstractObjectiveLiraglutide (LIRA) is a novel antidiabetic therapy that may have anti‐inflammatory and bone protective effects. Thus, we studied the potential therapeutic effect of LIRA on periodontitis by assessing the effects of LIRA on the proliferation, migration, inflammation, and osteogenic differentiation of human periodontal ligament cells (hPDLCs) after LPS stimulation.Material and MethodsThe expression of glucagon like‐peptide 1 receptor (GLP‐1R) was measured using qRT‐PCR. HPDLCs proliferation after LIRA were analyzed using MTT assays. Cell migration was quantified using a wound‐healing assay. The expression of inflammatory (IL‐6 and TNF‐α) was measured by qRT‐PCR and ELISA in hPDLCs. The effect of LIRA on the mineralization potential of hPDLCs was assessed by alizarin red S staining. Furthermore, the expression of Runx2 and ALP was measured by qRT‐PCR and Western blot in hPDLCs.ResultsGLP‐1R mRNA was present on hPDLCs, and LIRA increased the expression of GLP‐1R mRNA. When cultured with 25, 50, 75, 100 and 125 nM LIRA for 24 h, hPDLCs proliferation was enhanced in a dose‐dependent manner (P < 0.05), and 100 nM was optimal. LIRA promoted hPDLCs migration in a time‐dependent manner. LPS significantly increased the expression of IL‐6 and TNF‐α (P < 0.01), decreased the formation of mineralization nodes (P < 0.01), and inhibited the expression of ALP and Runx2 (P < 0.05). LIRA treatment blocked the expression of IL‐6 and TNF‐α (P < 0.01), increased the formation of mineralization nodes (P < 0.01), and enhanced the expression of ALP and Runx2 (P < 0.05).ConclusionLIRA can enhance the proliferation, migration, and osteogenic differentiation of hPDLCs and inhibit the inflammatory response. Thus, LIRA may have potential therapeutic use as an adjuvant treatment for human periodontitis, and this effect is independent of hypoglycemic activity.
Promises as Proposals in Joint Practical Deliberation
Authors: de Kenessey Brendan 
Published: September 2018

AbstractThis paper argues that promises are proposals in joint practical deliberation, the activity of deciding together what to do. More precisely: to promise to ϕ is to propose (in a particular way) to decide together with your addressee(s) that you will ϕ. I defend this deliberative theory by showing that the activity of joint practical deliberation naturally gives rise to a speech act with exactly the same properties as promises. A certain kind of proposal to make a joint decision regarding one's own actions turns out to have the very same normative effects, under the very same conditions, as a promise. I submit that this cannot be a coincidence: we should conclude that promises and the relevant kind of proposals in joint practical deliberation are one and the same.
Substitution of artificial colloids for fresh frozen plasma in pediatric cardiopulmonary bypass surgery
Authors: Wang Tian , Wang Xiangyu , Liu Jinping , Zou Lihua , Wang Jingtao , Zhao Mingxia , Cui Yongli 
Published: September 2018

SummaryBackgroundIn many centers, fresh frozen plasma is generally used as the main component of pump prime in pediatric cardiopulmonary bypass. However, many factors have resulted in stringent control of plasma transfusion and prompted the study of safe and efficient substitutes.AimsThe aim of this study was to investigate the feasibility of a priming strategy with gelatin during cardiopulmonary bypass in pediatric patients undergoing cardiac surgery and identify the factors associated with postoperative chest‐tube drainage.MethodsWe reviewed 1164 pediatric patients who underwent cardiac surgery with cardiopulmonary bypass between January 2012 and April 2013 in Fuwai hospital. Infants and children were primed with different types of solution: plasma or gelatin. Clinical data included postoperative coagulation function (pharmacological agents, chest‐tube drainage, and transfusion requirements), recovery indicators (mechanical ventilator time, ICU stay and hospital stay), incidence of in‐hospital mortality, and morbidity. Multivariate linear regression analysis was used to identify factors correlated with postoperative chest‐tube drainage.ResultsNo difference in mortality or morbidity was found between the plasma and gelatin groups. In infants, increased chest‐tube drainage (postoperation 12 hours, median difference −0.046 ml/kg/hr, 95%CI: −0.105 to −0.007, P = 0.001; postoperation 24 hours, median difference −0.047 ml/kg/hr, 95%CI: −0.081 to −0.025, P < 0.001), and decreased transfusion (red blood cell, median difference 0.00 ml/kg/hr, 95%CI: 0.000‐100, P < 0.001; fresh frozen plasma, median difference 5.556 ml/kg/hr, 95%CI: 2.30‐8.333, P = 0.001), and recovery time (mechanical ventilator time, median difference 3.00 hours, 95%CI: 1.00‐5.500, P < 0.001; ICU stay, median difference 17.00 hours, 95%CI: 1.00‐22.000, P = 0.001; hospital stay, median difference 1.00 day, 95%CI: 0.00‐2.000, P = 0.038) were demonstrated in the gelatin group. In children, the transfusion requirements (red blood cell, median difference 100 ml, P < 0.001;fresh frozen plasma, median difference 1.11 ml/kg, 95%CI: 0.000‐2.42, P = 0.001) were decreased in the gelatin group. Multivariate linear regression analysis revealed that the type of priming solution (β = 1.940,95%CI: 1.057‐2.823,P < 0.001), bypass time (β = 0.024, 95%CI: 0.013‐0.036, P < 0.001), and age (β = −0.257, 95%CI: −0.422 to −0.09, P = 0.002) were independent variables correlating with chest‐tube drainage in infants.ConclusionIn the general pediatric patients undergoing elective cardiac surgery, substitution of gelatin for fresh frozen plasma in cardiopulmonary bypass is feasible.
Vaccines in pediatric transplant recipients—Past, present, and future
Authors: Dulek Daniel E. , de St. Maurice Annabelle , Halasa Natasha B. 
Published: September 2018

AbstractInfections significantly impact outcomes for solid organ and hematopoietic stem cell transplantation in children. Vaccine‐preventable diseases contribute to morbidity and mortality in both early and late posttransplant time periods. Several infectious diseases and transplantation societies have published recommendations and guidelines that address immunization in adult and pediatric transplant recipients. In many cases, pediatric‐specific studies are limited in size or quality, leading to recommendations being based on adult data or mixed adult‐pediatric studies. We therefore review the current state of evidence for selected immunizations in pediatric transplant recipients and highlight areas for future investigation. Specific attention is given to studies that enrolled only children.
Developing Predictive Models to Enhance Clinician Prediction of Suicide Attempts Among Veterans With and Without PTSD
Authors: Barnes Sean M. , Monteith Lindsey L. , Forster Jeri E. , Nazem Sarra , Borges Lauren M. , Stearns‐Yoder Kelly A. , Bahraini Nazanin H. 
Published: September 2018

The limitations of self‐report confine clinicians’ ability to accurately predict suicides and suicide attempts (SAs). Behavioral assessments (e.g., Death Implicit Association Test [IAT]) may be a means of supplementing self‐report and clinician prediction.ObjectiveThe authors aimed to build and test a predictive model of SAs that included established risk factors and measures of suicide risk, and Death IAT scores. The authors also sought to test the predictive validity of the SA model among subgroups of Veterans with and without PTSD.MethodParticipants included 166 psychiatrically hospitalized Veterans.ResultsA model that included patient prediction, age, and Death IAT scores improved upon clinician prediction of SAs during the six‐month follow‐up (C‐statistic for clinician prediction = 73.6, 95% CI [62.9, 84.4] and C‐statistic for model = 82.8, 95% CI [74.6, 91.0]). The model was tested in subgroups of Veterans with and without PTSD. Among Veterans without PTSD, the Death IAT and patient prediction predicted SAs above and beyond clinician prediction, while these variables did not significantly improve prediction among Veterans with PTSD (C‐statistic for no‐PTSD = 91.3, 95% CI [80.6, 1.00]; C‐statistic for PTSD = 86.8, 95% CI [76.8, 96.8]). Building a separate model for Veterans with PTSD did not improve upon clinician prediction.ConclusionsFindings indicate that predictive models may bolster clinician prediction of SAs and that predictors may differ for Veterans with PTSD.
Oral cannabinoid-rich THC/CBD cannabis extract for secondary prevention of chemotherapy-induced nausea and vomiting: a study protocol for a pilot and definitive randomised double-blind placebo-controlled trial (CannabisCINV)
Authors: Mersiades Antony J , Tognela Annette , Haber Paul S , Stockler Martin , Lintzeris Nicholas , Simes John , McGregor Iain , Olver Ian , Allsop David J , Gedye Craig , Kirby Adrienne C , Morton Rachael L , Fox Peter , Clarke Stephen , Briscoe Karen , Aghmesheh Morteza , Wong Nicole , Walsh Anna , Hahn Carmel , Grimison Peter 
Published: September 2018

IntroductionChemotherapy-induced nausea and vomiting (CINV) remains an important issue for patients receiving chemotherapy despite guideline-consistent antiemetic therapy. Trials using delta-9-tetrahydrocannabinol-rich (THC) products demonstrate limited antiemetic effect, significant adverse events and flawed study design. Trials using cannabidiol-rich (CBD) products demonstrate improved efficacy and psychological adverse event profile. No definitive trials have been conducted to support the use of cannabinoids for this indication, nor has the potential economic impact of incorporating such regimens into the Australian healthcare system been established. CannabisCINV aims to assess the efficacy, safety and cost-effectiveness of adding TN-TC11M, an oral THC/CBD extract to guideline-consistent antiemetics in the secondary prevention of CINV.Methods and analysisThe current multicentre, 1:1 randomised cross-over, placebo-controlled pilot study will recruit 80 adult patients with any malignancy, experiencing CINV during moderate to highly emetogenic chemotherapy despite guideline-consistent antiemetics. Patients receive oral TN-TC11M (THC 2.5mg/CBD 2.5 mg) capsules or placebo capsules three times a day on day −1 to day 5 of cycle A of chemotherapy, followed by the alternative drug regimen during cycle B of chemotherapy and the preferred drug regimen during cycle C. The primary endpoint is the proportion of subjects attaining a complete response to CINV. Secondary and tertiary endpoints include regimen tolerability, impact on quality of life and health system resource use. The primary assessment tool is patient diaries, which are filled from day −1 to day 5. A subsequent randomised placebo-controlled parallel phase III trial will recruit a further 250 patients.Ethics and disseminationThe protocol was approved by ethics review committees for all participating sites. Results will be disseminated in peer-reviewed journals and at scientific conferences.Drug supplyTilray.Protocol version2.0, 9 June 2017.Trial registration numberANZCTR12616001036404; Pre-results.
Relationships among perceived quality of healthcare services, satisfaction and behavioural intentions of international students in Kuala Lumpur, Malaysia: a cross-sectional study
Authors: Aljaberi Musheer Abdulwahid , Juni Muhamad Hanafiah , Al-Maqtari Rasheed Addulsalam , Lye Munn Sann , Saeed Murad Abdu , Al-Dubai Sami Abdo Radman , Kadir Shahar Hayati 
Published: September 2018

ObjectiveThe current study aimed to investigate the relationships among the perceived quality of healthcare services, satisfaction and behavioural intentions among international students in Kuala Lumpur, Malaysia.SettingA cross-sectional study on public universities in Kuala Lumpur, Malaysia.ParticipantsAll participants in this study were international students joining public universities in Kuala Lumpur, Malaysia.Primary independent variablePerceived quality of healthcare services measured on a SERVQUAL scale.Primary dependent variableParticipants’ satisfaction of healthcare services assessed using five items.Secondary dependent variableBehavioural intentions measured on six items.ResultsOf the 556 international students, 500 (90%) completed the survey. The study used structural equation modelling (SEM) through the AMOS (Analysis of Moment Structures) software to analyse the data. SEM analyses showed that the perceived quality of healthcare services significantly and directly affected satisfaction, with a value of 89% and an effect size of 0.78. It also had a significant indirect impact on the behavioural intentions (0.78) of international students. The results indicated that participants’ satisfaction had a statistically significant impact on their behavioural intentions (0.77).ConclusionPerceived quality of care is an important driver of international students’ satisfaction and their behavioural intention with healthcare services. Such relations of effects among the three investigated factors were also positive and significant.
What are the requirements for developing a successful national registry of auditory implants? A qualitative study
Authors: Mandavia Rishi , Knight Alec , Carter Alexander W , Toal Connor , Mossialos Elias , Littlejohns Peter , Schilder Anne GM 
Published: September 2018

ObjectivesHearing loss is an area of unmet need, and industry is targeting this field with a growing range of surgically implanted hearing devices. Currently, there is no comprehensive UK registry capturing data on these devices; in its absence, it is difficult to monitor clinical and cost-effectiveness and develop national policy. Recognising that developing such a registry faces considerable challenges, it is important to gather opinions from stakeholders and patients. This paper builds on our systematic review on surgical registry development and aims to identify the specific requirements for developing a successful national registry of auditory implants.DesignQualitative study.ParticipantsData were collected in two ways: (1) semistructured interviews with UK professional stakeholders; and (2) focus groups with patients with hearing loss. The interview and focus group schedules were informed by our systematic review on registry development. Data were analysed using directed content analysis. Judges mapped the themes obtained against a conceptual framework developed from our systematic review on registry development. The conceptual framework consisted of five categories for successful registry development: (1) planning, (2) registry governance, (3) registry dataset, (4) anticipating challenges, (5) implementing solutions.ResultsTwenty-seven themes emerged from 40 semistructured interviews with professional stakeholders and 18 themes emerged from three patient focus groups. The most important factor for registry success was high rates of data completion. Benefits of developing a successful registry of auditory implants include: strengthening the evidence base and regulation of auditory implants, driving quality and safety improvements, increased transparency, facilitating patient decision-making and informing policy and guidelines development.ConclusionsThis study identifies the requirements for developing a successful national registry of auditory implants, benefiting from the involvement of numerous professional stakeholder groups and patients with hearing loss. Our approach may be used internationally to inform successful registry development.
Associations between prolactin receptor (PRLR) polymorphisms and milk production traits in dairy buffalo
Authors: Al-Kal Abakar , Jiang Qin Yang , Qin Guang Sheng , Pan Bin , Chen Bao Jian , Jiang He Sheng 
Published: September 2018

Buffalo milk is economically important, and an understanding of the genetic basis of milk production traits may facilitate buffalo breeding for this purpose. In this study, polymerase chain reaction-single strand conformation polymorphism was used to analyze polymorphisms in prolactin receptor (PRLR) exons 3, 5, 7, and 10 in Murrah, Nili-Ravi, and crossbred buffaloes and Holstein cows. A site in PRLR exon 3 exhibited variation in all three buffalo breeds and cows, and the three genotypes GG, GT, and TT were detected. In PRLR exon 5, nucleotide differences were detected between buffalo and Holstein dairy cow populations; the genotypes AA, AG, and GG were observed. In PRLR exon 7, polymorphisms were not detected. In PRLR exon 10, at 9637 bp, the TT, TC, and TT genotypes were observed. At the three polymorphic loci, Hardy–Weinberg equilibrium was reached in the crossbred buffalo population for exons 3 and 5 and in all three buffalo populations for exon 10. In an analysis of the associations of the three polymorphisms with milk production traits, only PRLR exon 10 in Murrah buffaloes was associated with milk yield, with a significant difference between the TT and CC genotypes.
Context-dependent tactile texture-sensitivity in monkey M1 and S1 cortex
Authors: Jiang Wan , Tremblay François , Chapman C. Elaine 
Published: September 2018

Caudal primary motor cortex (M1, area 4) is sensitive to cutaneous inputs, but the extent to which the physical details of complex stimuli are encoded is not known. We investigated the sensitivity of M1 neurons (4 Macaca mulatta monkeys) to textured stimuli (smooth/rough or rough/rougher) during the performance of a texture discrimination task and, for some cells, during a no-task condition (same surfaces; no response). The recordings were made from the hemisphere contralateral to the stimulated digits; the motor response (sensory decision) was made with the nonstimulated arm. Most M1 cells were modulated during surface scanning in the task (88%), but few of these were texture-related (24%). In contrast, 44% of M1 neurons were texture related in the no-task condition. Recordings from the neighboring primary somatosensory cortex (S1), the potential source of texture-related signals to M1, showed that S1 neurons were significantly more likely to be texture related during the task (57 vs 24%) than M1. No difference was observed in the no-task condition (52 vs. 44%). In these recordings, the details about surface texture were relevant for S1 but not for M1. We suggest that tactile inputs to M1 were selectively suppressed when the animals were engaged in the task. S1 was spared these controls, as the same inputs were task-relevant. Taken together, we suggest that the suppressive effects are most likely exerted directly at the level of M1, possibly through the activation of a top-down gating mechanism specific to motor set/intention. NEW & NOTEWORTHY Sensory feedback is important for motor control, but we have little knowledge of the contribution of sensory inputs to M1 discharge during behavior. We showed that M1 neurons signal changes in tactile texture, but mainly outside the context of a texture discrimination task. Tactile inputs to M1 were selectively suppressed during the task because this input was not relevant for the recorded hemisphere, which played no role in generating the discrimination response.
Predicting responses to inhibitory synaptic input in substantia nigra pars reticulata neurons
Authors: Simmons D. V. , Higgs M. H. , Lebby S. , Wilson C. J. 
Published: September 2018

The changes in firing probability produced by a synaptic input are usually visualized using the poststimulus time histogram (PSTH). It would be useful if postsynaptic firing patterns could be predicted from patterns of afferent synaptic activation, but attempts to predict the PSTH from synaptic potential waveforms using reasoning based on voltage trajectory and spike threshold have not been successful, especially for inhibitory inputs. We measured PSTHs for substantia nigra pars reticulata (SNr) neurons inhibited by optogenetic stimulation of striato-nigral inputs or by matching artificial inhibitory conductances applied by dynamic clamp. The PSTH was predicted by a model based on each SNr cell’s phase-resetting curve (PRC). Optogenetic activation of striato-nigral input or artificial synaptic inhibition produced a PSTH consisting of an initial depression of firing followed by oscillatory increases and decreases repeating at the SNr cell’s baseline firing rate. The phase resetting model produced PSTHs closely resembling the cell data, including the primary pause in firing and the oscillation. Key features of the PSTH, including the onset rate and duration of the initial inhibitory phase, and the subsequent increase in firing probability could be explained from the characteristic shape of the SNr cell’s PRC. The rate of damping of the late oscillation was explained by the influence of asynchronous phase perturbations producing firing rate jitter and wander. Our results demonstrate the utility of phase-resetting models as a general method for predicting firing in spontaneously active neurons and their value in interpretation of the striato-nigral PSTH. NEW & NOTEWORTHY The coupling of patterned presynaptic input to sequences of postsynaptic firing is a Gordian knot, complicated by the multidimensionality of neuronal state and the diversity of potential initial states. Even so, it is fundamental for even the simplest understanding of network dynamics. We show that a simple phase-resetting model constructed from experimental measurements can explain and predict the sequence of spike rate changes following synaptic inhibition of an oscillating basal ganglia output neuron.
Multiple Routes to Oncogenesis Are Promoted by the Human Papillomavirus–Host Protein Network
Authors: Eckhardt Manon , Zhang Wei , Gross Andrew M. , Von Dollen John , Johnson Jeffrey R. , Franks-Skiba Kathleen E. , Swaney Danielle L. , Johnson Tasha L. , Jang Gwendolyn M. , Shah Priya S. , Brand Toni M. , Archambault Jacques , Kreisberg Jason F. , Grandis Jennifer R. , Ideker Trey , Krogan Nevan J. 
Published: September 2018

Abstract
We have mapped a global network of virus–host protein interactions by purification of the complete set of human papillomavirus (HPV) proteins in multiple cell lines followed by mass spectrometry analysis. Integration of this map with tumor genome atlases shows that the virus targets human proteins frequently mutated in HPV− but not HPV+ cancers, providing a unique opportunity to identify novel oncogenic events phenocopied by HPV infection. For example, we find that the NRF2 transcriptional pathway, which protects against oxidative stress, is activated by interaction of the NRF2 regulator KEAP1 with the viral protein E1. We also demonstrate that the L2 HPV protein physically interacts with the RNF20/40 histone ubiquitination complex and promotes tumor cell invasion in an RNF20/40-dependent manner. This combined proteomic and genetic approach provides a systematic means to study the cellular mechanisms hijacked by virally induced cancers.
Significance: In this study, we created a protein–protein interaction network between HPV and human proteins. An integrative analysis of this network and 800 tumor mutation profiles identifies multiple oncogenesis pathways promoted by HPV interactions that phenocopy recurrent mutations in cancer, yielding an expanded definition of HPV oncogenic roles. Cancer Discov; 8(11); 1474–89. ©2018 AACR.
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