bluuebunny/crossref_metadata_2025_split huggingface dataset:
Healthcare Satisfaction and Unmet Needs Among Bereaved Parents in the NICU
Authors: Baughcum Amy E. , Fortney Christine A. , Winning Adrien M. , Dunnells Zackery D. O. , Humphrey Lisa M. , Gerhardt Cynthia A. 
Published: September 2019

Background:
Learning directly from bereaved parents about their experiences in the neonatal intensive care unit (NICU) can improve services at end-of-life (EOL) care. Parents who perceive that their infant suffered may report less satisfaction with care and may be at greater risk for distress after the death. Despite calls to improve EOL care for children, limited research has examined the EOL experiences of families in the NICU.

Purpose:
We examined parent perceptions of their infant's EOL experience (eg, symptom burden and suffering) and satisfaction with care in the NICU.

Methods/Search Strategy:
Forty-two mothers and 27 fathers (representing 42 infants) participated in a mixed-methods study between 3 months and 5 years after their infant's death (mean = 39.45 months, SD = 17.19). Parents reported on healthcare satisfaction, unmet needs, and infant symptoms and suffering in the final week of life.

Findings/Results:
Parents reported high levels of healthcare satisfaction, with relative strengths in providers' technical skills and inclusion of the family. Greater perceived infant suffering was associated with lower healthcare satisfaction and fewer well-met needs at EOL. Parents' understanding of their infant's condition, emotional support, communication, symptom management, and bereavement care were identified as areas for improvement.

Implications for Practice:
Parents value comprehensive, family-centered care in the NICU. Additionally, monitoring and alleviating infant symptoms contribute to greater parental satisfaction with care. Improving staff knowledge about EOL care and developing structured bereavement follow-up programs may enhance healthcare satisfaction and family outcomes.

Implications for Research:
Prospective studies are needed to better understand parental perceptions of EOL care and the influence on later parental adjustment.
A Prospective Study of Leukocyte Telomere Length and Risk of Gestational Diabetes in a Multiracial Cohort
Authors: Lin Yuan , Zhu Yeyi , Wu Jing , Hinkle Stefanie N. , Rawal Shristi , Han Jiali , Weir Natalie L. , Tsai Michael Y. , Zhang Cuilin 
Published: October 2019

Background:
Short telomere length (TL), an indicator of cellular aging and oxidative stress, has been implicated in glucose homeostasis. Additionally, studies have illustrated that the association of TL with health outcomes may vary by age. Yet, data on the association between TL and gestational diabetes mellitus (GDM) are sparse and the potential effect modification by age remains unknown.

Methods:
We prospectively investigated TL in early pregnancy in relation to the subsequent GDM risk in a case–control study of 93 women with GDM and 186 randomly selected controls matched on age, race/ethnicity, and gestational weeks at blood collection. TL was measured using blood samples collected at 10–14 gestational weeks and reported as the T/S ratio, a ratio of telomere repeat length T to copy number of a single copy gene S. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using conditional logistic regression adjusted for major risk factors.

Results:
Overall, TL was not significantly associated with GDM risk. The TL–GDM association was significantly modified by age (P
interaction = 0.02). Shorter TL in early pregnancy was associated with an increased GDM risk among women <30 years old (adjusted OR comparing the shortest vs. longest tertile: 3.1, 95% CI = 1.2, 8.1), but not associated with GDM risk among women ≥30 years.

Conclusion:
Our findings suggest that TL in early pregnancy may be implicated in GDM development, particularly among younger women.
Delayed Hypersensitivity Reaction after Nipple Tattooing: A Novel Case Report
Authors: Joseph Walter J. , Roy Eva , Stofman Guy M. 
Published: October 2019

Summary:
We present a patient who underwent bilateral 3-dimensional (3D) nipple tattooing for nipple areolar reconstruction after implant-based breast reconstruction for breast cancer. Several weeks after nipple tattooing, the patient developed a delayed hypersensitivity reaction around both of her tattooed nipple areolar complexes. This is the first case reported in the literature of a hypersensitivity reaction from 3D nipple tattooing.
Fire Ant Punctate Keratopathy: A Novel Diagnosis Based on Clinical and Anterior Segment Optical Coherence Tomography Findings
Authors: Patael Yael Yohai , Segal Ori , Segev Fani 
Published: September 2019

Purpose:
To describe the ocular findings and the long-term outcomes of patients diagnosed with corneal injury due to little fire ants (LFAs).

Methods:
This is a retrospective case series of patients evaluated with corneal injury due to LFAs from October 2015 to January 2018 at the Cornea Clinic in Meir Medical Center. Patients underwent anterior segment optical coherence tomography (AS-OCT) imaging during the follow-up.

Results:
Four patients reported ocular contact with LFAs and presented to our department with unilateral, scattered, small, dense, round, bright white opacities in the anterior corneal stroma, which remained unchanged in appearance over time, despite topical antibiotic and steroid treatment. No inflammatory signs were noted in the anterior chamber. Follow-up was performed clinically using AS-OCT for periods of 9 to 26 months after the incidents. AS-OCT findings demonstrated small, subepithelial, hyperreflective foci at the level of the anterior stroma to a depth of 145 to 250 μm, with posterior shadowing that remained unchanged over time. These findings were well-correlated with biomicroscopy findings.

Conclusions:
LFA bites can cause long-lasting corneal injury in humans with characteristic clinical biomicroscopic findings. The corneal lesions do not respond to conventional topical treatment. AS-OCT imaging can be a useful modality for diagnosis and follow-up. Awareness by both patients and ophthalmologists of this clinical entity may be helpful in diagnosis and management.
Upregulation of cortical GABAA receptor concentration in fibromyalgia
Authors: Pomares Florence B. , Roy Steve , Funck Thomas , Feier Natasha A. , Thiel Alexander , Fitzcharles Mary-Ann , Schweinhardt Petra 
Published: September 2019

Abstract
An imbalance between excitatory and inhibitory neurotransmission has been linked to fibromyalgia (FM). Magnetic resonance spectroscopy has shown increased levels of glutamate in the insula and posterior cingulate cortex in FM as well as reduced insular levels of gamma-aminobutyric acid (GABA). Both of these changes have been associated with increased pain sensitivity. However, it is not clear whether excitatory and/or inhibitory neurotransmission is altered across the brain. Therefore, the aim of this study was to quantify GABAA receptor concentration on the whole brain level in FM to investigate a potential dysregulation of the GABAergic system. Fifty-one postmenopausal women (26 FM, 25 matched controls) underwent assessments of pain sensitivity, attention and memory, psychological status and function, as well as positron emission tomography imaging using a tracer for GABAA receptors, [18F]flumazenil. Patients showed increased pain sensitivity, impaired immediate memory, and increased cortical GABAA receptor concentration in the attention and default-mode networks. No decrease of GABAA receptor concentration was observed. Across the 2 groups, GABAA receptor concentration correlated positively with functional scores and current pain in areas overlapping with regions of increased GABAA receptor concentration. This study shows increased GABAA receptor concentration in FM, associated with pain symptoms and impaired function. The changes were widespread and not restricted to pain-processing regions. These findings suggest that the GABAergic system is altered, possibly indicating an imbalance between excitatory and inhibitory neurotransmission. Future studies should try to understand the nature of the dysregulation of the GABAergic system in FM and in other pain syndromes.
Two successful insertions of peripherally inserted central catheters in a patient with persistent left superior vena cava
Authors: Kang Junren , Liu Bing , Sun Wenyan 
Published: October 2019

Abstract

Rationale:
Peripherally inserted central catheters (PICC), normally located at the lower 1/3rd of the superior vena cava (SVC) and cavo-atrial junction, are commonly used in cancer patients. Persistent left superior vena cava (PLSVC) is a vascular anomaly, in patients with which seldom research was reported about PICC implanted. After obtaining written informed consent, we present a case where two successful insertions of PICC were performed in a 50-year-old female patient with PLSVC and right SVC.

Patients concerns:
The patient had ovarian cancer and was admitted for chemotherapy using PICC.

Diagnoses:
Ovarian cancer and PLSVC.

Interventions and Outcomes:
Following insertion of PICC in PLSVC, thrombosis developed. PICC was removed after routine anticoagulation therapy. Owing to tumor recurrence, a second PICC was inserted in the right SVC without any complications.

Lessons:
PICC insertion in PLSVC for chemotherapy may be associated with an increased risk of deep venous thrombosis of the upper extremity. A right catheter insertion in patient with PLSVC was preferred.
Meta-analysis of FOXP3 gene rs3761548 and rs2232365 polymorphism and multiple sclerosis susceptibility
Authors: Zhang Yijian , Zhang Junxin , Liu Hao , He Fan , Chen Angela , Yang Huilin , Pi Bin 
Published: October 2019

Abstract

Background
Multiple sclerosis (MS) is a common autoimmune disease of the central nervous system (CNS), and is associated with genetic factors. FOXP3 gene polymorphism has been reported as the risk factor for MS, however, previous studies have showed conflicting results. The purpose of this study is to investigate the association between FOXP3 gene polymorphism and the susceptibility to MS.

Methods
Pubmed, Embase, library of Cochrane, and Web of Science were used to search the eligible articles from January 1980 up to October 2018. The odds ratio (ORs) and its 95% confidence intervals (CI) were used to evaluate the strength of association. Allele model, homozygote model, heterozygote model, dominant model, and recessive model were used to evaluate the association between FOXP3 gene polymorphism and MS.

Results
A total of 5 studies contained 1276 MS patients and 1447 controls (for rs3761548) and 600 MS patients and 640 controls (for rs2232365) were enrolled in this meta-analysis. The association showed significant differences in allele and dominant model for rs3761548 polymorphism. In addition, a clear tendency to significance was detected in homozygote and recessive model for rs3761548 (P = .052). Subgroup analysis indicated a significant risk of MS in all genotype models but heterozygotes in Asians.

Conclusion

FOXP3 gene polymorphism rs3761548 was associated with a higher MS risk, especially in Asians. This conclusion needs to be validated in more large samples and multiracial studies.

Level of evidence
Level III diagnostic study.
Inclusion of Nursing Homes and Long-term Residents in Medicare ACOs
Authors: Chang Chiang-Hua , Mainor Alexander , Raymond Stephanie , Peck Kristen , Colla Carrie , Bynum Julie 
Published: September 2019

Background:
Long-term nursing home residents have complex needs that often require services from acute care settings. The accountable care organization (ACO) model provides an opportunity to improve care by creating payment incentives for more coordinated, higher quality care.

Objectives:
To assess the extent of nursing home participation in ACOs, and the characteristics of residents and their nursing homes connected to ACOs.

Research Design:
This was a cross-sectional study.

Subjects:
Medicare nursing home residents identified from 2014 Minimum Data Set assessments. Residents were attributed to ACOs based on Medicare methods.

Measures:
Individuals’ demographics, clinical characteristics, health care utilization, and nursing home characteristics.

Results:
Among 660,780 nursing home residents, a quarter of them were attributed to ACOs. ACO residents had only small differences from non-ACO residents: age 85 years and older (47.1% vs. 45.3%), % black (10.5% vs. 12.7%), % dual eligible (74.3% vs. 75.8%), and emergency department visits (55.1 vs. 57.3 per 100). Of the 14,868 nursing homes with study residents, few were ACO providers (N=222, 1.6% of total residents) yet many had at least one ACO resident (N=8077, 76.4% of total residents); one-fifth had at least 20 (N=2839, 33.4% of total residents). ACO-provider homes were more likely than other homes to have a 5-star rating, be hospital-based and have Medicare as the primary payer.

Conclusions:
With a quarter of long-term nursing home residents attributed to an ACO, and one-fifth of nursing homes caring for a large number of ACO residents, outcomes and spending in this setting are important for ACOs to consider when designing patient care strategies.
Absence of the TRIP13 c.1060C>T Mutation in Wilms Tumor Patients From Pakistan
Authors: Rashid Muhammad U. , Naeemi Humaira , Qazi Abid Q. , Muhammad Noor , Ahmad Mansoor , Akhtar Noreen , Rahman Nazneen 
Published: October 2019

Background and Aim:
Wilms tumor (WT) is the most common childhood malignant renal tumor. Germline mutations in several WT predisposition genes have been identified. However, the fundamental cause of most WT patients remains unexplained. Recently, a founder mutation, c.1060C>T (p. Arg254X) in a mitotic spindle checkpoint gene, TRIP13, was reported in 5 unrelated children with WT from the United Kingdom, of Pakistani descent from Azad Kashmir region. This observation suggests other children with WT in Pakistan may also harbor this mutation. We conducted the first study to assess the contribution of TRIP13 c.1060C>T mutation to WT in Pakistan.

Materials and Methods:
Constitutional genomic DNA from 68 Pakistani individuals including unrelated WT cases (n=26) and one (n=10) or both (n=32) of their parent(s) were screened for the TRIP13 c.1060C>T mutation using DNA sequence analysis. We also included positive controls in the analyses.

Results:
The median age of WT diagnosis was 3.0 years (range, 0.75 to 10). The TRIP13 c.1060C>T mutation was not found in any WT patient (n=26) or their parents (n=42). Twenty-four patients (92.4%) presented with unilateral tumor and 2 patients (7.7%) were diagnosed with synchronous bilateral WT. Thirteen patients (50%) reported parental consanguinity. Thirteen patients (50.0%) belonged to the Punjabi ethnicity and 1 patient (3.8%) had a Kashmiri background. Four patients (16.7%) reported a family history of WT or other malignancies. The predominant histologic subtype was stromal (46.2%). The majority of patients presented with >5 cm of tumor size (81%). None of the patients had a personal or family history of congenital anomalies, or associated genetic syndromes.

Conclusions:
Our findings suggest that TRIP13 c.1060C>T mutation may be infrequent in Pakistani WT cases. Further evaluation of this mutation in a large number of WT patients of Kashmiri heritage and various ethnic backgrounds from Pakistan is warranted.
Integrin α6β4 signaling switches DNA repair from homologous recombination to non-homologous end-joining pathway to sensitize breast cancer cells to cisplatin
Authors: Chen Min , Marrs Brock , Qi Lei , Knifley Teresa , Jarrett Stuart G. , Weiss Heidi L. , Stewart Rachel L. , D’Orazio John A. , O’Connor Kathleen L. 
Published: September 2019

AbstractIntegrin α6β4 is highly expressed in triple negative breast cancer (TNBC) and drives aggressiveness by stimulating proliferation, angiogenesis, cell migration, invasion and metastasis. Signaling from this integrin stimulates DNA repair and apoptosis resistance, suggesting that it could contribute to therapeutic resistance. Upon testing this hypothesis, we found that integrin α6β4 signaling promoted a three-fold greater sensitivity to cisplatin but exhibited no difference in response to other chemotherapies tested. Mechanistic investigations revealed that integrin α6β4 stimulated quicker and higher amplitude of activation of ATM, Chk2, p53, and 53BP1, which required the integrin β4 signaling domain. Genetic manipulation of gene expression demonstrated that mutant p53 cooperated with integrin α6β4 for cisplatin sensitivity and was necessary for downstream phosphorylation of 53BP1 and enhanced ATM activation. Additionally, we discovered that integrin α6β4 preferentially activated DNA-PKc in response to cisplatin, which led to formation of DNA-PKc-p53 complexes and 53BP1 activation. As a result, integrin α6β4 shifted double strand break repair from homologous recombination (HR) to non-homologous end joining (NHEJ). In summary, we discovered a novel function of integrin α6β4 in switching DSB repair from HR to NHEJ that results in cisplatin sensitivity in TNBC.
Neural reinstatement guides context-dependent emotional memory retrieval
Authors: Hennings Augustin C. , McClay Mason , Lewis-Peacock Jarrod A. , Dunsmoor Joseph E. 
Published: October 2019

AbstractReactivating the memory of a context improves retrieval of information learned in that context. But does context reactivation resolve memory competition between related but conflicting emotional experiences? Here, we asked whether spontaneously retrieved episodic context disambiguates competing memories of fear and safety in the healthy brain and in posttraumatic stress disorder. During fMRI, subjects learned that items from an object category were a threat, and then learned that different items from the same category were safe in a unique context. The next day, subjects viewed new threat ambiguous stimuli from the same category and reported their expectation of threat. Multivoxel pattern analysis (MVPA) was used to identify and decode neural patterns unique to the context of safety learning. We show that in healthy adults the degree of neural reinstatement of the safe context predicted activity to threat ambiguous stimuli in the ventromedial prefrontal cortex (vmPFC) and hippocampus, regions important for contextual modulation of emotional memory retrieval. The degree of context reinstatement also disambiguated subjects’ feelings of threat versus safety. In contrast, context reinstatement did not resolve emotional memory retrieval in subjects with PTSD, indicating a contextual processing deficit in this group. Finally, multivoxel pattern similarity between safe and threat ambiguous stimuli overlapped in the vmPFC in healthy adults but not in PTSD. These findings reveal how neural reinstatement helps resolve context-dependent memory retrieval between opposing sources of emotional information. Understanding how the human brain separately encodes and retrieves competing memories of related events has broad implications for the study of emotional memory, associative learning, and the neuropathophysiology of affective disorders.
ITC and SPR Analysis Using Dynamic Approach
Authors: Krishnamoorthy Ganesh Kumar , Alluvada Prashanth , Hameed Shahul , Kwa Timothy , Krishnamoorthy Janarthanan 
Published: October 2019

ABSTRACTBiophysical techniques such as Isothermal Calorimetry (ITC) and Surface Plasmon Resonance (SPR) are routinely used to ascertain the global binding mechanisms of protein-protein or protein-ligand interaction. Recently, Dumas etal, have explicitly modelled the instrument response of the ligand dilution and analysed the ITC thermogram to obtain kinetic rate constants. Adopting a similar approach, we have integrated the dynamic instrument response with the binding mechanism to simulate the ITC profiles of equivalent and independent binding sites, equivalent and sequential binding sites and aggregating systems. The results were benchmarked against the standard commercial software Origin-ITC. Further, the experimental ITC chromatograms of 2’-CMP + RNASE and BH3I-1 + hBCLXLinteractions were analysed and shown to be comparable with that of the conventional analysis. Dynamic approach was applied to simulate the SPR profiles of a two-state model, and could reproduce the experimental profile accurately.
Evolution of face-centered-cubic polycrystalline plastic anisotropy under biaxial loading by crystal plasticity finite element method
Authors: Zhao Dan , Xin Cun , Jin Tao , Yan Xiaopeng , Ma Shenggguo , Wang Zhihua 
Published: October 2019

Purpose
The purpose of this study to analyze the plastic anisotropy of 6061 aluminum alloy with finite deformation using crystal plasticity finite element method.

Design/methodology/approach
A representative volume element (RVE) model was constructed by Voronoi tessellation. In this model, grain shapes, grain orientations and distribution of grains were involved. The mechanical response of the component under composite loading was tested using specify cruciform specimen. Moreover, different stress and strain states in the specific central region were analyzed to reveal the effects of complex loading.

Findings
We calculated the influence of misorientation of adjacent grains as well as the evolution of the micro structure’s plastic deformation on the macroscopic deformation of the structure. Geometry design for the cruciform specimen helps obtain a homogenous distribution of the stress and strain at the specimen center. In this process, the initial grain orientation is also an important factor, and the larger misorientations between special grains may cause greater stress concentration.

Originality/value
The influence of micro-scale factors on macro-scale plastic anisotropy of AA6061 is analyzed using RVE model and cruciform specimen, and they offer a reference for related research.