bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiac stress biomarkers after red blood cell transfusion in patients at risk for transfusion‐associated circulatory overload: a prospective observational study
Authors: Callum Jeannie L. , Cohen Robert , Cressman Alex M. , Strauss Rachel , Armali Chantal , Lin Yulia , Pendergrast Jacob , Lieberman Lani , Scales Damon C. , Skeate Robert , Ross Heather , Cserti‐Gazdewich Christine 
Published: September 2018

BACKGROUNDTransfusion‐associated circulatory overload (TACO) is a leading cause of serious reactions. In regard to TACO, little is known regarding biomarkers as a predictor, their most informative timing, or thresholds of significance or differentiation from other reactions.STUDY DESIGN AND METHODSIn this study of inpatients at risk for TACO (age ≥ 50 years) receiving 1 red blood cell unit, cardiac biomarkers, brain natriuretic peptide (BNP), N‐terminal pro‐BNP (NT‐proBNP), and high‐sensitivity troponin were measured at baseline, 6 to 12 hours (except troponin) posttransfusion, and 18 to 24 hours posttransfusion. Primary outcome was a critical increase in biomarkers (>1.5‐fold increase and supranormal) at 18 to 24 hours.RESULTSFifty‐one patients were analyzed; 29% had cardiovascular disease, 73% had one or more cardiac risk factors, and 50% took cardiac or antihypertensive therapies. Although eight (16%) developed an increase in systolic pressure of at least 30 mmHg and four (8%) reported dyspnea and/or cough, none had TACO. At baseline, BNP level was more than 100 ng/L in 59% and NT‐proBNP was more than 300 pg/mL in 83%. A total of 25% had a BNP critical increase, 33% had a NT‐proBNP critical increase, and 2% had a troponin critical increase at 18 to 24 hours. Overall, 38% had at least one biomarker critical increase and NT‐proBNP/BNP concordance was 84%. An increase in the NT‐proBNP (>1.5‐fold increase and >300 pg/mL) at 18 to 24 hours was the commonest biomarker change.CONCLUSIONSAn increase of the NT‐proBNP at 18 to 24 hours may be the preferred surrogate marker for identifying a patient experiencing physiologic difficulty in handling the volume challenge. Larger studies are needed to clarify the risk of TACO for a given pretransfusion biomarker profile and the correlation between TACO and increase in biomarkers after transfusion.
The role of social media for blood donor motivation and recruitment
Authors: Sümnig Ariane , Feig Martin , Greinacher Andreas , Thiele Thomas 
Published: September 2018

BACKGROUNDSocial media platforms have become an important lifestyle aspect. Therefore, we implemented communication via social media platforms to recruit new donors and to motivate repeat donors. Here, we report a survey among donors of our blood donation facility to evaluate the impact of different strategies for donor motivation from the donors’ perspective.STUDY DESIGN AND METHODSDuring 8 consecutive weeks, all whole blood donors were asked to participate. The survey consisted of a questionnaire including the demographic items sex, age, number of prior donations, and 14 potential motivators for blood donation. Social media included the items “Facebook” and “Jodel” (German local mobile application).RESULTSOf 3320 consecutive donors, 2920 (88%) participated in the survey. Social media motivated 7.4% of our donors, among them mainly young and female donors. For first‐time donors (FTDs; n = 157) the three strongest motivational factors were friends and/or relatives (73%), social media (15%), and “I do not need additional motivation” (11%). Repeat donors (n = 2693) most often stated that they do not need additional motivation (72%) and only 7% were motivated by social media.CONCLUSIONSocial media have become the second most important motivator to recruit FTDs beside relatives and friends who are by far the main motivators for FTDs. For repeat donors, social media play a less important role. Social media are becoming increasingly important for transfusion services.
In vivo viability of extended 4°C‐stored autologous apheresis platelets
Authors: Stolla Moritz , Fitzpatrick Lynda , Gettinger Irena , Bailey Shawn L. , Pellham Esther , Christoffel Todd , Slichter Sherrill J. 
Published: September 2018

BACKGROUNDThe current 5‐day storage time of room temperature (22°C)‐stored platelets (RSPs) severely limits platelet (PLT) availability. Extended cold (4°C)‐stored PLTs (CSPs) are currently being investigated for actively bleeding patients. However, we currently do not know how to best store PLTs in the cold for extended periods of time. In this study, we investigate how storage in plasma and PLT additive solutions (PASs) affects PLT viability in vivo.STUDY DESIGN AND METHODSTwenty normal subjects had a 2‐unit hyperconcentrated apheresis PLT collection. One unit was stored at 4°C in plasma for 3 days (“control unit”), and the CSP “test” unit was stored for 10 or 15 days in plasma or 10 days in 35% plasma with either 65% Intersol or Isoplate. After storage, all units were radiolabeled and transfused into their donors.RESULTSFor 10‐day storage, both the plasma and the Intersol units had significantly better PLT recoveries than the Isoplate units (24% ± 8% vs. 11% ± 3% [55% ± 11% vs. 21% ± 8% as percentage of control data], p = 0.002; and 18% ± 4% vs. 11% ± 3% [43% ± 6% vs. 21% ± 8% as percentage of control data], p = 0.004, respectively). There was a trend for lower PLT recoveries with Intersol compared to plasma (p = 0.056). PLT survivals and most in vitro measurements did not differ significantly among the units.CONCLUSIONSWhile the in vitro variables suggest largely comparable results between plasma and PASs, in vivo recoveries were higher with plasma compared with both Intersol and Isoplate (p = 0.057 and p = 0.002, respectively). Whether this difference leads to clinically relevant differences in hemostatic efficacy remains to be determined.
Iron overload is correlated with impaired autologous stem cell mobilization and survival in acute myeloid leukemia
Authors: Alva Laura C. , Bacher Ulrike , Seipel Katja , Mansouri Taleghani Behrouz , Mueller Beatrice U. , Novak Urban , Pabst Thomas 
Published: September 2018

BACKGROUNDPatients with acute myeloid leukemia (AML) undergoing consolidation with autologous stem cell transplantation (ASCT) depend on the successful mobilization of peripheral blood stem cells. However, the factors affecting the mobilization potential in AML patients and, in particular, the effect of transfusion‐related iron overload on peripheral blood stem cell mobilization are largely unknown.STUDY DESIGN AND METHODSWe investigated the association of varying levels of iron overload and stem cell mobilization efficacy in consecutive AML patients after two induction cycles.RESULTSA total of 113 AML patients in early first complete remission underwent the mobilization procedure. While 84 (74.3%) patients had serum ferritin levels exceeding 1000 μg/L, 26 (23.0%) patients had levels even higher than 2000 μg/L. Iron overload correlated with the number of preceding red blood cell transfusions and inversely correlated with circulating CD34+ cell levels (p = 0.04) at apheresis. Finally, the median progression‐free and overall survival rates of patients with ferritin levels of higher than 2000 μg/L were shorter with 332 days versus 2156 days (p = 0.04) and 852 days versus 2235 days (p = 0.04), respectively.CONCLUSIONOur data suggest that transfusion‐related iron overload is suppressing the mobilization potential and is associated with inferior outcome in AML.
Circulating miRNA or circulating DNA—Potential biomarkers for organ transplant rejection
Authors: Huo Qin , Zhou Ming , Cooper David K. C. , Dai Yifan , Xie Ni , Mou Lisha 
Published: September 2018

AbstractAccurate and timely diagnosis of transplant rejection is essential for the long‐term survival of solid‐organ transplant recipients. Biopsy is the “gold standard” for the diagnosis of rejection, but this approach is a time‐consuming, expensive, and invasive process. An efficient, cheap, and noninvasive tool for monitoring of immune rejection is urgently needed. Circulating miRNA and circulating DNA can be easily amplified and quantified by PCR which could be potential biomarkers for monitoring organ survival and/or immune rejection. In this review, we briefly introduce the origins and characteristics of circulating miRNA and circulating DNA. Furthermore, we review their clinical application and potential as noninvasive biomarkers for rejection in organ transplantation. Finally, we sum up their potential as biomarkers, which may help scientists and surgeons choose the proper biomarker for routine assays.
Hypothalamic DNA methylation in rats with dihydrotestosterone‐induced polycystic ovary syndrome: effects of low‐frequency electro‐acupuncture
Authors: Cui Peng , Ma Tong , Tamadon Amin , Han Sha , Li Bing , Chen Zheyi , An Xiaofei , Shao Linus R. , Wang Yanqing , Feng Yi 
Published: September 2018

New Findings
What is the central question of this study?
What is the role of hypothalamic DNA methylation in the development of polycystic ovary syndrome (PCOS) and the response to electro‐acupuncture treatment.
What is the main finding and its importance?
Global DNA methylation and expression of DNA methyltransferases (DNMTs) were increased in PCOS‐like rats, and electro‐acupuncture (EA) decreased global DNA methylation and DNMT3b expression. Pyrosequencing showed that the DNA methylation of some PCOS candidate genes was changed in the PCOS and PCOS+EA groups, suggesting that hypothalamic DNA methylation plays an important role in the development of PCOS and in mediating the effects of electro‐acupuncture treatment.
AbstractPolycystic ovary syndrome (PCOS) is a common reproductive and endocrine disease of unknown aetiology. Recently, epigenetic studies focusing on DNA methylation in PCOS have received much attention, but the mechanisms are still unclear. In the present study, we used the 5α‐dihydrotestosterone‐induced PCOS‐like rat model and treated the rats with electro‐acupuncture (EA). Rats were randomly divided into four groups – controls, diet‐induced obesity, PCOS and PCOS+EA. We examined the reproductive, metabolic and behavioural phenotypes, validated the effect of EA, and explored the role of hypothalamic DNA methylation by analysing the methylation of global DNA and selected candidate genes. The PCOS rats presented with reproductive dysfunctions such as lack of regular oestrous cyclicity, metabolic disorders such as increased body weight and insulin resistance, and depression and anxiety‐like behaviours. EA improved the reproductive functions, decreased body weight and improved experimental depressive behaviour. Furthermore, global DNA methylation and the expression of DNA methyltransferases (DNMTs) were increased in PCOS rats compared to the control group, and EA decreased the global DNA methylation and the expression of DNMT3b. In addition, pyrosequencing showed that the DNA methylation of certain CpG sites in targeted genes (Plcg1, Camk2b, Esr2 and Pgr) was increased in the PCOS group, but the DNA methylation of Camk2b and Ar was decreased after EA treatment. These results indicate that hypothalamic DNA methylation might be correlated with the development of PCOS and that EA has an effect on hypothalamic DNA methylation in PCOS rats.
Effects of Concentric and Eccentric Strength Training on Fatigue Induced by Concentric and Eccentric Exercises
Authors: Cadore Eduardo Lusa , González-Izal Miriam , Grazioli Rafael , Setuain Igor , Pinto Ronei Silveira , Izquierdo Mikel 
Published: September 2018

Purpose: To compare the concentric and eccentric training effects on fatigue induced by eccentric and concentric protocols. Methods: A total of 22 men and women (22 [3.6] y) were assigned to concentric (GCON, n = 11) or eccentric training (GECC, n = 11). The concentric (CON) and eccentric (ECC) protocols were composed of 4 sets of 20 knee-extension/flexion repetitions. Force losses were analyzed by comparing 10 repetitions’ mean torques during the protocols and by verifying the maximal voluntary contraction and rate of torque development before and after the protocols. Muscle damage was assessed using echo intensity of the vastus lateralis 48 h after the protocols. Training consisted of 6 wk of isokinetic exercise at 60°/s (concentric or eccentric) twice weekly. Results: Before training, both protocols resulted in dynamic and isometric force losses in GCON and GECC (P < .01), but the magnitude was greater after the CON protocol than after the ECC protocol (P < .001). After training, both GCON and GECC showed similar force decreases during the CON and ECC protocols (P < .01), and these changes were not different from the pretraining decreases. Regarding maximal voluntary contraction after training, GECC showed lower force decreases than GCON after ECC exercise (−13.7% vs −22.3%, respectively, P < .05), whereas GCON showed lower maximal voluntary contraction decreases after CON exercise compared with pretraining (−29.2%, P < .05). Losses in rate of torque development were similar after the protocols before and after the training regimens. No changes in echo intensity were observed after the protocols before and after training. Conclusion: Both interventions resulted in similar force decreases during fatigue protocols compared with those associated with pretraining.
Interunit Reliability and Effect of Data-Processing Methods of Global Positioning Systems
Authors: Thornton Heidi R. , Nelson André R. , Delaney Jace A. , Serpiello Fabio R. , Duthie Grant M. 
Published: September 2018

Purpose: To establish the interunit reliability of a range of global positioning system (GPS)-derived movement indicators, to determine the variation between manufacturers, and to investigate the difference between software-derived and raw data. Methods: A range of movement variables were obtained from 27 GPS units from 3 manufacturers (GPSports EVO, 10 Hz, n = 10; STATSports Apex, 10 Hz, n = 10; and Catapult S5, 10 Hz, n = 7) that measured the same team-sport simulation session while positioned on a sled. The interunit reliability was determined using the coefficient of variation (%) and 90% confidence limits, whereas between-manufacturers comparisons and comparisons of software versus raw processed data were established using standardized effect sizes and 90% confidence limits. Results: The interunit reliability for both software and raw processed data ranged from good to poor (coefficient of variation = 0.2%; ±1.5% to 78.2%; ±1.5%), with distance, speed, and maximal speed exhibiting the best reliability. There were substantial differences between manufacturers, particularly for threshold-based acceleration and deceleration variables (effect sizes; ±90% confidence limits: −2.0; ±0.1 to 1.9; ±0.1), and there were substantial differences between data-processing methods for a range of movement indicators. Conclusions: The interunit reliability of most movement indicators was deemed as good regardless of processing method, suggesting that practitioners can have confidence within systems. Standardized data-processing methods are recommended, due to the large differences between data outputs from various manufacturer-derived software.
Load–Velocity Relationship in National Paralympic Powerlifters: A Case Study
Authors: Loturco Irineu , Pereira Lucas A. , Winckler Ciro , Santos Weverton L. , Kobal Ronaldo , McGuigan Michael 
Published: September 2018

Purpose: To examine the relationships between different loading intensities and movement velocities in the bench-press exercise (BP) in Paralympic powerlifters. Methods: A total of 17 national Paralympic powerlifters performed maximum dynamic strength tests to determine their BP 1-repetition maximum (1RM) in a Smith-machine device. A linear position transducer was used to measure movement velocity over a comprehensive range of loads. Linear-regression analysis was performed to establish the relationships between the different bar velocities and the distinct percentages of 1RM. Results: Overall, the correlations between bar velocities and %1RM were strong over the entire range of loads (R2 .80–.91), but the precision of the predictive equations (expressed as mean differences [%] between actual and predicted 1RM values) were higher at heavier loading intensities (∼20% for loads ≤70% 1RM and ∼5% for loads ≥70% 1RM). In addition, it seems that these very strong athletes (eg, 1RM relative in the BP = 2.22 [0.36] kg·kg−1, for male participants) perform BP 1RM assessments at lower velocities than those previously reported in the literature. Conclusions: The load–velocity relationship was strong and consistent in Paralympic powerlifters, especially at higher loads (≥70% 1RM). Therefore, Paralympic coaches can use the predictive equations and the reference values provided here to determine and monitor the BP loading intensity in national Paralympic powerlifters.
Evaluation of the Psychometric Properties of the Persian Version of the Test of Gross Motor Development–3rd Edition
Authors: Mohammadi Farzad , Bahram Abbas , Khalaji Hasan , Ulrich Dale A. , Ghadiri Farhad 
Published: September 2018

The Test of Gross Motor Development–3rd Edition (TGMD-3) is an instrument for measuring gross motor development in children with and without a disability. This study aims to evaluate the psychometric properties of the Persian version of the TGMD-3 in 3- to 10-year-old Iranian children. The TGMD-3 was administered to 1,600 children (M age = 6.56 ± 2.29 years; 50% boys). The content validity of the TGMD-3 was established by five experts while its reliability was assessed through calculating internal consistency, test–retest, intra-rater, and inter-rater reliability coefficients. All reliability indices were excellent (>.82). The two-factor model was validated using confirmatory factor analysis. Adequate fit indices were found for the two-factor model (χ2 (64) = 389.02, p < .05, root mean square error of approximation (RMSEA) = .056, goodnesss of fit index (GFI) = .96, adjusted goodness of fit index (AGFI) = .94, normed fit index (NFI) = .96, non-normed fit index (NNFI) = .96, comparative fit index (CFI) = .96, incremental fit index (IFI) = .96, standardized root mean square residual (SRMR) = .03). An alternative one-factor model was also tested. Adequate fit indices in a one-factor model were found (χ2 (65) = 615.88, p = .0001, RMSEA = .07, GFI = .94, AGFI = .92, NFI = .98, NNFI = .98, CFI = .98, IFI = .98, SRMR = .03). The psychometric properties of the Persian version of TGMD-3 in Iranian children were supported and users can confidently use this test to evaluate gross motor development in Iranian children.
Association between serum magnesium concentration and metabolic syndrome, diabetes, hypertension and hyperuricaemia in knee osteoarthritis: a cross-sectional study in Hunan Province, China
Authors: Wang Yilun , Wei Jie , Zeng Chao , Yang Tuo , Li Hui , Cui Yang , Xie Dongxing , Xu Bei , Liu Zhichen , Li Jiatian , Jiang Shide , Lei Guanghua 
Published: September 2018

ObjectivesTo examine the associations between serum magnesium (Mg) concentration with the prevalence of metabolic syndrome (MetS), diabetes mellitus (DM), hypertension (HP) and hyperuricaemia (HU) in patients with radiographic knee osteoarthritis (OA).MethodsThe present study was conducted at the Health Management Center of Xiangya Hospital. Radiographic OA was evaluated for patients aged over 40 years with basic characteristics and blood biochemical assessment. Serum Mg concentration was measured using the chemiluminescence method. MetS, DM, HP and HU were diagnosed based on standard protocols. The associations between serum Mg concentration with MetS, DM, HP and HU were evaluated by conducting multivariable adjusted logistic regression.ResultsA total of 962 patients with radiographic knee OA were included. Compared with the lowest quintile, the multivariable adjusted ORs and related 95% CIs of DM were 0.40 (95% CI 0.23 to 0.70, p=0.001), 0.33 (95% CI 0.18 to 0.60, p<0.001), 0.27 (95% CI 0.14 to 0.52, p<0.001) and 0.22 (95% CI 0.11 to 0.44, p<0.001) in the second, third, fourth and highest quintiles of serum Mg, respectively (p for trend <0.001); the multivariable adjusted ORs of HU were 0.33 (95% CI 0.19 to 0.59, p<0.001), 0.52 (95% CI 0.30 to 0.91, p=0.022) and 0.39 (95% CI 0.22 to 0.70, p=0.001) in the third, fourth and highest quintiles of serum Mg, respectively (p for trend <0.001); and the multivariable adjusted ORs of MetS were 0.59 (95% CI 0.36 to 0.94, p=0.027) in the second and 0.56 (95% CI 0.34 to 0.93, p=0.024) in the highest quintiles of serum Mg. However, the inverse association between serum Mg and the prevalence of MetS was non-linear (p for trend=0.067). There was no significant association between serum Mg and HP in patients with OA.ConclusionsThe serum Mg concentration was inversely associated with the prevalence of MetS, DM and HU in patients with radiographic knee OA.Level of evidenceLevel III, cross-sectional study.
Clinical features, bacteriology of endotracheal aspirates and treatment outcomes of patients with chronic obstructive pulmonary disease and community-acquired pneumonia in an intensive care unit in Taiwan with an emphasis on eosinophilia versus non-eosinophilia: a retrospective case–control study
Authors: Huang Wei-Chang , Lee Ching-Hsiao , Wu Ming-Feng , Huang Chen-Cheng , Hsu Cheng-Hui , Chen Hui-Chen , Hsu Jeng-Yuan , Huang Chieh-Chen 
Published: September 2018

ObjectivesThe clinical implications of blood eosinophil level in patients with chronic obstructive pulmonary disease (COPD) and community-acquired pneumonia (CAP) requiring invasive mechanical ventilation (IMV) and intensive care unit (ICU) admission are still unknown. Thus, this study aimed to compare the features of such patients with and without blood eosinophilia.DesignThis was a retrospective case–control study.SettingAn ICU of a medical centre in central Taiwan.ParticipantsA total of 262 patients with COPD and CAP requiring IMV and ICU admission.ResultsOf all participants (n=262), 32 (12.2%) had an eosinophil percentage (EP) >2% and 169 (64.5%) had an absolute eosinophil count (AEC) >300 cells/µL. Regardless of whether 2% or 300 cells/µL was used as a cut-off value, the eosinophilia group were slightly older (years) (82.9±5.4 vs 78.1±9.1, p=0.000 and 79.2±8.4 vs 77.6±9.6, p=0.246, respectively), and had a higher forced expiratory volume in 1 s/forced vital capacity (%) (56.0±8.0 vs 51.3±11.6, p=0.005 and 53.1±11.2 vs 49.5±11.2, p=0.013, respectively), less severe spirometric classification (p=0.008 and p=0.001, respectively), and lower white cell count 109/L (8.8±3.2 vs 11.1±4.9, p=0.009 and 10.3±4.4 vs 11.8±5.3, p=0.017, respectively) than the non-eosinophilia group. The bacteriology of endotracheal aspirates showed thatPseudomonas aeruginosaand other gram-negative bacilli were the most common organisms in all study groups. Participants with an EP >2% had a shorter ICU length of stay (OR=12.13, p=0.001) than those with an EP ≤2%, while an AEC >300 cells/µL was not associated with any in-ICUoutcomes.ConclusionsThe results of this study have significant clinical implications and should be considered when making treatment decisions for the management of patients with COPD and CAP requiring IMV and ICU admission.