bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential PI(4,5)P2 sensitivities of TRPC4, C5 homomeric and TRPC1/4, C1/5 heteromeric channels
Authors: Ko Juyeon , Myeong Jongyun , Shin Young-Cheul , So Insuk 
Published: February 2019

AbstractTransient receptor potential canonical (TRPC) 4 and TRPC5 channels are modulated by the Gαq-PLC pathway. Since phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) maintains TRPC4 and TRPC5 channel function, the Gαq-PLC pathway inhibits channel activity by depleting PI(4,5)P2. Here we investigated the difference in PI(4,5)P2 sensitivity between homomeric and heteromeric TRPC channels. First, by using a Danio rerio voltage-sensing phosphatase (DrVSP), we show that PI(4,5)P2 dephosphorylation robustly inhibits TRPC4α, TRPC4β, and TRPC5 homotetramer currents and also TRPC1/4α, TRPC1/4β, and TRPC1/5 heterotetramer currents. Secondly, sensitivity of channels to PI(4,5)P2 dephosphorylation was suggested through the usage of FRET in combination with patch clamping. The sensitivity increased in the sequence TRPC4β < TRPC4α < TRPC5 in homotetramers, whereas when forming heterotetramers with TRPC1, the sensitivity was approximately equal between the channels. Thirdly, we determined putative PI(4,5)P2 binding sites based on a TRPC4 prediction model. By neutralization of basic residues, we identified putative PI(4,5)P2 binding sites because the mutations reduced FRET to a PI(4,5)P2 sensor and reduced the current amplitude. Therefore, one functional TRPC4 has 8 pockets with the two main binding regions; K419, K664/R511, K518, H630. We conclude that TRPC1 channel function as a regulator in setting PI(4,5)P2 affinity for TRPC4 and TRPC5 that changes PI(4,5)P2 sensitivity.
A galling insect activates plant reproductive programs during gall development
Authors: Schultz Jack C. , Edger Patrick P. , Body Mélanie J. A. , Appel Heidi M. 
Published: February 2019

AbstractMany insect species have acquired the ability to redirect plant development to form unique organs called galls, which provide these insects with unique, enhanced food and protection from enemies and the elements. Many galls resemble flowers or fruits, suggesting that elements of reproductive development may be involved. We tested this hypothesis using RNA sequencing to quantify the transcriptional responses of wild grapevine (Vitis riparia) leaves to a galling parasite, phylloxera (Daktulosphaira vitifoliae). If development of reproductive structures is part of gall formation, we expected to find significantly elevated expression of genes involved in flower and/or fruit development in developing galls as opposed to ungalled leaves. We found that reproductive gene ontology categories were significantly enriched in developing galls, and that expression of many candidate genes involved in floral development were significantly increased, particularly in later gall stages. The patterns of gene expression found in galls suggest that phylloxera exploits vascular cambium to provide meristematic tissue and redirects leaf development towards formation of carpels. The phylloxera leaf gall appears to be phenotypically and transcriptionally similar to the carpel, due to the parasite hijacking underlying genetic machinery in the host plant.
Low noise, open-source QEPAS system with instrumentation amplifier
Authors: Winkowski Mateusz , Stacewicz Tadeusz 
Published: February 2019

AbstractQuartz enhanced photoacoustic spectroscopy (QEPAS) is a rapidly developing, ultrasensitive method for trace gas sensing. Adequate electronic amplifier, well matched to the quartz characteristics is crucial for overall system performance. Here we present an open source circuit for QEPAS signal amplification. It consists of a buffer, instrumentation amplifier and digitally controlled gain stage. An experiment showed, that it offers signal to noise ratio of about 23 dB better than commonly used transimpedance amplifier. The use of this circuit provides opportunity to improve QEPAS sensitivity by about one order of magnitude.
The genetic and epigenetic association of LDL Receptor Related Protein 1B (LRP1B) gene with childhood obesity
Authors: Lee Suman 
Published: February 2019

AbstractLow-density lipoprotein Receptor Related Protein 1B (LRP1B) is homologous to the gigantic lipoprotein receptor-related protein 1 that belongs to the family of Low-density lipoprotein receptors. Previous genetic association studies of the LRP1B gene have shown its genetic association with obesity. Through exome sequencing of the LRP1B gene from a childhood severe obesity cohort (n = 692), we found novel single nucleotide polymorphism (rs431809) in intron 4, which has been significantly correlated with both body mass index (BMI) and waist-hip-ratio (WHR). Three methylations of CpG sites (cg141441481, cg01852095 and cg141441470) in the same intron were also significantly correlated with BMI and WHR. All CpG methylations had bimodal patterns, and were dependent on rs431809 genotypes. The genetic influences of obesity on the LRP1B gene may be linked to the interplay of CpG methylations in the same intron. Heritability of SNP interacts with epigenetic crosstalk in LRP1B. Genetic and epigenetic crosstalk of LRP1B gene may be implicated in the prevention and therapeutic approach to childhood obesity.
Rapid and reversible lithiation of doped biogenous iron oxide nanoparticles
Authors: Misawa Masaaki , Hashimoto Hideki , Kalia Rajiv K. , Matsumoto Syuji , Nakano Aiichiro , Shimojo Fuyuki , Takada Jun , Tiwari Subodh , Tsuruta Kenji , Vashishta Priya 
Published: February 2019

AbstractCertain bacteria produce iron oxide material assembled with nanoparticles (NPs) that are doped with silicon (Fe:Si ~ 3:1) in ambient environment. Such biogenous iron oxides (BIOX) proved to be an excellent electrode material for lithium-ion batteries, but underlying atomistic mechanisms remain elusive. Here, quantum molecular dynamics simulations, combined with biomimetic synthesis and characterization, show rapid charging and discharging of NP within 100 fs, with associated surface lithiation and delithiation, respectively. The rapid electric response of NP is due to the large fraction of surface atoms. Furthermore, this study reveals an essential role of Si-doping, which reduces the strength of Li-O bonds, thereby achieving more gentle and reversible lithiation culminating in enhanced cyclability of batteries. Combined with recent developments in bio-doping technologies, such fundamental understanding may lead to energy-efficient and environment-friendly synthesis of a wide variety of doped BIOX materials with customized properties.
A mind-brain-body dataset of MRI, EEG, cognition, emotion, and peripheral physiology in young and old adults
Authors: Babayan Anahit , Erbey Miray , Kumral Deniz , Reinelt Janis D. , Reiter Andrea M. F. , Röbbig Josefin , Schaare H. Lina , Uhlig Marie , Anwander Alfred , Bazin Pierre-Louis , Horstmann Annette , Lampe Leonie , Nikulin Vadim V. , Okon-Singer Hadas , Preusser Sven , Pampel André , Rohr Christiane S. , Sacher Julia , Thöne-Otto Angelika , Trapp Sabrina , Nierhaus Till , Altmann Denise , Arelin Katrin , Blöchl Maria , Bongartz Edith , Breig Patric , Cesnaite Elena , Chen Sufang , Cozatl Roberto , Czerwonatis Saskia , Dambrauskaite Gabriele , Dreyer Maria , Enders Jessica , Engelhardt Melina , Fischer Marie Michele , Forschack Norman , Golchert Johannes , Golz Laura , Guran C. Alexandrina , Hedrich Susanna , Hentschel Nicole , Hoffmann Daria I. , Huntenburg Julia M. , Jost Rebecca , Kosatschek Anna , Kunzendorf Stella , Lammers Hannah , Lauckner Mark E. , Mahjoory Keyvan , Kanaan Ahmad S. , Mendes Natacha , Menger Ramona , Morino Enzo , Näthe Karina , Neubauer Jennifer , Noyan Handan , Oligschläger Sabine , Panczyszyn-Trzewik Patricia , Poehlchen Dorothee , Putzke Nadine , Roski Sabrina , Schaller Marie-Catherine , Schieferbein Anja , Schlaak Benito , Schmidt Robert , Gorgolewski Krzysztof J. , Schmidt Hanna Maria , Schrimpf Anne , Stasch Sylvia , Voss Maria , Wiedemann Annett , Margulies Daniel S. , Gaebler Michael , Villringer Arno 
Published: February 2019

AbstractWe present a publicly available dataset of 227 healthy participants comprising a young (N=153, 25.1±3.1 years, range 20–35 years, 45 female) and an elderly group (N=74, 67.6±4.7 years, range 59–77 years, 37 female) acquired cross-sectionally in Leipzig, Germany, between 2013 and 2015 to study mind-body-emotion interactions. During a two-day assessment, participants completed MRI at 3 Tesla (resting-state fMRI, quantitative T1 (MP2RAGE), T2-weighted, FLAIR, SWI/QSM, DWI) and a 62-channel EEG experiment at rest. During task-free resting-state fMRI, cardiovascular measures (blood pressure, heart rate, pulse, respiration) were continuously acquired. Anthropometrics, blood samples, and urine drug tests were obtained. Psychiatric symptoms were identified with Standardized Clinical Interview for DSM IV (SCID-I), Hamilton Depression Scale, and Borderline Symptoms List. Psychological assessment comprised 6 cognitive tests as well as 21 questionnaires related to emotional behavior, personality traits and tendencies, eating behavior, and addictive behavior. We provide information on study design, methods, and details of the data. This dataset is part of the larger MPI Leipzig Mind-Brain-Body database.
A stream classification system for the conterminous United States
Authors: McManamay Ryan A. , DeRolph Christopher R. 
Published: February 2019

AbstractStream classifications are important for understanding stream ecosystem diversity while also serving as tools for aquatic conservation and management. With current rates of land and riverscape modification within the United States (US), a comprehensive inventory and evaluation of naturally occurring stream habitats is needed, as this provides a physical template upon which stream biodiversity is organized and maintained. To adequately represent the heterogeneity of stream ecosystems, such a classification needs to be spatially extensive where multiple stream habitat components are represented at the highest resolution possible. Herein, we present a multi-layered empirically-driven stream classification system for the conterminous US, constructed from over 2.6 million stream reaches within the NHDPlus V2 stream network. The classification is based on emergent natural variation in six habitat layers meaningful at the stream-reach resolution: size, gradient, hydrology, temperature, network bifurcation, and valley confinement. To support flexibility of use, we provide multiple alternative approaches to developing classes and report uncertainty in classes assigned to stream reaches. The stream classification and underlying data provide valuable resources for stream conservation and research.
Understanding the electrical, thermal, and mechanical properties of epoxy magnesium oxide nanocomposites
Authors: Peddamallu Nagachandrika , Nagaraju Guvvala , Sridharan Krishnamurth , Velmurugan Ramachandran , Vasa Nilesh Jayantilal , Nakayama Tadachika , Sarathi Ramanujam 
Published: February 2019

Epoxy MgO nanocomposites exhibit high surface discharge inception voltage (SDIV) in SF6 gas ambience. Increase in SDIV is observed with 3wt% of MgO‐added epoxy nanocomposites, especially when the SF6 ambient gas pressure is high. Impact of thermal ageing of epoxy nanocomposites on SDIV is minimum. Dielectric response studies were carried out experimentally and compared with modelling studies. The surface potential decay and charge trap characteristics of epoxy nanocomposites vary with percentage of nano filler. Thermal aging of epoxy nanocomposites has less influence on surface potential decay characteristics for 1 and 3 wt% MgO‐added epoxy nanocomposites, indicating anti‐thermal aging characteristics. Surface discharge activity generates UHF with its dominant frequency at around 1 GHz. The UHF signal magnitude formed due to SD activity is minimum with 3 wt% MgO‐added epoxy nanocomposites. Laser‐induced breakdown spectroscopy (LIBS) studies indicate that plasma temperature and threshold fluence increases with increase in wt% of MgO in epoxy resin. Tensile and flexural properties are greatly improved for nanocomposite compared to epoxy. The dynamic mechanical analysis (DMA) indicates increased storage modulus and reduction of tan δ with epoxy nanocomposites. The glass transition temperature (Tg) and activation energy increases with wt % of nano filler.
Synthesis and characterisation of VG nanosheets on silica aerogel by plasma‐enhanced chemical vapour deposition method
Authors: Vigneswari S. , Murugesan S. 
Published: February 2019

Vertical graphene (VG) nanosheets are directly grown on silica aerogel and silicon substrate (100) by plasma‐enhanced chemical vapour deposition process in the presence of hydrogen as a reducing agent at atmospheric pressure at 800°C. The physiochemical properties of the nanohybrid were thoroughly characterised by Raman spectroscopy, scanning electron microscopy (SEM) and atomic force microscopy (AFM). The presence of full wave half maximum of D, G and D′ indicates VG nanowalls. The height (≅50 nm) of the VG nanowalls were confirmed by SEM and Raman spectroscopy but AFM reveals it as ≅ 15 nm. Surface and cross‐sectional SEM show the uniform distribution of vertical growth of nanowalls and the AFM revealed surface morphology of VG nanowalls.
Ultrasonic bonding method controlled by the characteristic waveform of ultrasonic propagation
Authors: Sun Yibo , Teng Tiandong , Guo Guoqing , Wu Guoxiong 
Published: February 2019

The vibration propagated in polymer components is related to the interfacial fusion in ultrasonic bonding process, which can be employed to be reference variables to control the ultrasonic energy. In this paper, an ultrasonic precise bonding method controlled by characteristic waveform from the wavelet decomposition of the ultrasonic propagation is proposed. The vibration propagated from ultrasonic horn, through polymer components, to the anvil finally is analysed by wavelet packet decomposition. The node signal in the frequency band of 109,375–125,000 Hz, which contains more information related to the interfacial fusion behaviour, is reconstructed as the characteristic waveform. The ultrasonic bonding control procedure is designed by the recognition of two characteristic points on the characteristic waveform. Results indicate that, at the second characteristic point, high‐quality interfacial fusion bonding is achieved without overflow out of the edge.
Plasmonic nano bow‐tie arrays with enhanced LSPR refractive index sensing
Authors: Khoshdel Vahid , Shokooh‐Saremi Mehrdad 
Published: February 2019

In this work, the performance of the nano bow‐tie arrays for biosensing applications has been investigated. Since the geometrical parameters of the device such as the bow‐tie length and gap size have major effects on the system's optical response, a parametric study based on finite‐difference time‐domain (FDTD) method has been conducted. In order to enhance the sensitivity, two bow‐tie shaped nanoparticles in the form of 2 × 1 and 2 × 2 arrays have been developed. In the case of TM polarisation, a sensitivity up to 923 nm/RIU with a figure of merit (FOM) of 5.5 has been obtained for 2 × 2 nano bow‐tie array. Moreover, the performance of the proposed nanoparticles for TE polarisation has been evaluated and a FOM exceeding 15.9 has been achieved for single nano bow‐tie. According to this study, the proposed nano bow‐tie structures have a great potential for sensing and detection applications.
Graphene nanosheets‐Inconel 718 nanocomposites fabricated by spark plasma sintering of in‐situ grown vertically standing graphene nanosheets‐Inconel 718 powders
Authors: Chen Dongsheng , Zhou Haitao , Tian Ye , Luo Fei , Liu Dabo , Luo Bingwei 
Published: February 2019

The present work reports a mechanical improvement in nickel‐based nanocomposites reinforced by in‐situ grown graphene nanosheets (GNSs). First, vertically standing GNSs (VSGNSs) were grown on Inconel 718 alloy (718) powders by plasma‐enhanced chemical vapour deposition. Then these powders were used to fabricate GNSs‐718 nanocomposites by using spark plasma sintering method. The microstructure and mechanical properties of the nanocomposites were investigated by Raman spectroscopy, scanning electron microscopy, transmission electron microscopy and tensile tests. Testing results showed that three‐dimensional net structured VSGNSs were in‐situ grown on surfaces of 718 powders. GNSs‐718 nanocomposites exhibited homogeneously dispersed GNSs, which distributed mainly along grain boundaries. Transmission electron microscopy indicated that there were clean and strong interfaces between GNSs and nickel‐based matrix. Compared to the unreinforced 718 alloy, GNSs‐718 nanocomposites showed increased strength and elongation. The relevant strengthening mechanisms involved in GNSs‐718 nanocomposites were discussed.
The CARMA-NRO Orion Survey
Authors: Suri Sümeyye , Sánchez-Monge Álvaro , Schilke Peter , Clarke Seamus D. , Smith Rowan J. , Ossenkopf-Okada Volker , Klessen Ralf , Padoan Paolo , Goldsmith Paul , Arce Héctor G. , Bally John , Carpenter John M. , Ginsburg Adam , Johnstone Doug , Kauffmann Jens , Kong Shuo , Lis Dariusz C. , Mairs Steve , Pillai Thushara , Pineda Jaime E. , Duarte-Cabral Ana 
Published: February 2019

Context. We present an initial overview of the filamentary structure in the Orion A molecular cloud utilizing a high angular and velocity resolution C18O(1–0) emission map that was recently produced as part of the CARMA-NRO Orion Survey.
Aims. The main goal of this study is to build a credible method to study varying widths of filaments which has previously been linked to star formation in molecular clouds. Due to the diverse star forming activities taking place throughout its ~20 pc length, together with its proximity of 388 pc, the Orion A molecular cloud provides an excellent laboratory for such an experiment to be carried out with high resolution and high sensitivity.
Methods. Using the widely-known structure identification algorithm, DisPerSE, on a three-dimensional (PPV) C18O cube, we identify 625 relatively short (the longest being 1.74 pc) filaments over the entire cloud. We studied the distribution of filament widths using FilChaP, a python package that we have developed and made publicly available.
Results. We find that the filaments identified in a two square-degree PPV cube do not overlap spatially, except for the complex OMC-4 region that shows distinct velocity components along the line of sight. The filament widths vary between 0.02 and 0.3 pc depending on the amount of substructure that a filament possesses. The more substructure a filament has, the larger is its width. We also find that despite this variation, the filament width shows no anticorrelation with the central column density which is in agreement with previous Herschel observations.
Galactic Archaeology with asteroseismic ages: Evidence for delayed gas infall in the formation of the Milky Way disc
Authors: Spitoni E. , Silva Aguirre V. , Matteucci F. , Calura F. , Grisoni V. 
Published: February 2019

Context. Precise stellar ages from asteroseismology have become available and can help to set stronger constraints on the evolution of the Galactic disc components. Recently, asteroseismology has confirmed a clear age difference in the solar annulus between two distinct sequences in the [α/Fe] versus [Fe/H] abundance ratios relation: the high-α and low-α stellar populations.
Aims. We aim to reproduce these new data with chemical evolution models including different assumptions for the history and number of accretion events.
Methods. We tested two different approaches: a revised version of the “two-infall” model where the high-α phase forms by a fast gas accretion episode and the low-α sequence follows later from a slower gas infall rate, and the parallel formation scenario where the two disc sequences form coevally and independently.
Results. The revised two-infall model including uncertainties in age and metallicity is capable of reproducing: i) the [α/Fe] versus [Fe/H] abundance relation at different Galactic epochs, ii) the age−metallicity relation and the time evolution [α/Fe]; iii) the age distribution of the high-α and low-α stellar populations, iv) the metallicity distribution function. The parallel approach is not capable of properly reproducing the stellar age distribution, in particular at old ages.
Conclusions. The best chemical evolution model is the revised two-infall one, where a consistent delay of ∼4.3 Gyr in the beginning of the second gas accretion episode is a crucial assumption to reproduce stellar abundances and ages.
STEP as a Useful Tool to Screen for Diabetes-Specific Health-Related
Problems in Community-Based Geriatric Patients- An Exploratory Secondary
Analysis of Cross-Sectional Data
Authors: Carmienke Solveig , Geier Anne-Kathrin , Deutsch Tobias , Rasche Franz Maximilian , Frese Thomas 
Published: February 2019

Abstract
Background and Aims Comprehensive geriatric assessments are established
tools for the identification of health problems in the elderly. So far, little
is known about tailoring and targeting to facilitate their application. As a
starting point for a tailored assessment of patients with diabetes, a highly
prevalent health condition, we aimed to assess if the Standardized Assessment of
Elderly People (STEP) is able to identify relevant differences in self-reported
health problems between diabetic and non-diabetic patients.
Patients and Methods We performed a secondary analysis of a
cross-sectional study including 1007 adults (aged 65 and older) from 28 German
general practices, evaluating the feasibility and usefulness of the
self-administered STEP version. For this exploratory study we re-analysed the
data and compared patients with and without diabetes.
Results Out of 940 patients included in the secondary analysis, 248
(26.4%) had diabetes. Compared to non-diabetic patients, geriatric
diabetic patients reported more often problems in activities of daily living,
physical problems typically associated with diabetes such as urinary
incontinence, visual impairment, mood disturbances, as well as the use of
medical or social services. Most of our results were stable after adjusting for
age, sex and body mass index.
Conclusion We conclude that the self-administered version of the STEP
tool may be used to screen for health problems typically associated with
diabetes. Our results may guide the development of a tailored STEP-version
specifically for diabetic patients. Further research might evaluate the adoption
and usefulness of such a tool in every-day general practice.
Kutane nichttuberkulöse Mykobakteriosen
Authors: Emmerich K. , Kolb-Mäurer A. , Goebeler M. 
Published: February 2019

ZusammenfassungAtypische Mykobakterien sind eine heterogene Gruppe ubiquitär vorkommender Umwelt-Saprophyten, die zu oberflächlichen und tiefen Hautinfektionen bis hin zu Hautinfektionen mit Systembeteiligung führen können. Die Erreger vermögen nach Bagatellverletzungen in die Haut einzudringen und führen insbesondere bei Immunsupprimierten zu schweren Infektionen. Klinisch werden schnell und langsam wachsende atypische Mykobakterien unterschieden. Für die Dermatologie wichtige Vertreter der langsam wachsenden Mykobakterien sind M. marinum, M. ulcerans und M. haemophilum. M. marinum verursacht das Schwimmbad- bzw. Aquariumgranulom, die in westlichen Industriestaaten häufigste atypische Mykobakteriose der Haut. M. ulcerans ist der Erreger der weltweit häufigsten kutanen atypischen Mykobakteriose und verursacht v. a. in Westafrika das sog. Buruli-Ulkus. M. haemophilum wird besonders bei Immunsupprimierten und bei Kindern mit begleitender zervikaler und perihilärer Lymphadenitis beobachtet. Zu den schnellwachsenden atypischen Mykobakterien gehören M. abscessus, M. chelonae und M. fortuitum. Relevant sind sie insbesondere als Verursacher von kutanen Mykobakteriosen, die in Zusammenhang mit iatrogener Immunsuppression sowie medizinischen und kosmetischen Prozeduren stehen. Das Management von NTM stellt eine besondere Herausforderung dar: Das klinische Erscheinungsbild ist vielfältig, die Diagnostik schwierig und die antibiotische Kombinationstherapie nicht standardisiert.