bluuebunny/crossref_metadata_2025_split huggingface dataset:
Advanced paternal age diversifies individual trajectories of vocalization patterns in neonatal mice
Authors: Mai Lingling , Inada Hitoshi , Kimura Ryuichi , Kanno Kouta , Matsuda Takeru , Tachibana Ryosuke O. , Tucci Valter , Komaki Fumiyasu , Hiroi Noboru , Osumi Noriko 
Published: August 2019

SummaryInfant crying is an innate communicative behavior that is frequently impaired in certain neurodevelopmental disorders (NDDs). Since advanced paternal age is a reported risk factor for NDDs in offspring, we evaluated the impact of a father’s age on early vocal development in C57BL/6J mice. We recorded and applied a unique combination of computational analyses to ultrasonic vocalizations (USVs) emitted by mouse pups sired by young and aged fathers. Our data showed that advanced paternal age reduced the number and duration of USVs, and altered the syllable composition in pups. Moreover, pups born to young fathers showed convergent vocal characteristics with a rich repertoire during postnatal development, while those born to aged fathers exhibited more divergent vocal patterns with limited repertoire. Principal component analysis in conjunction with clustering analysis demonstrated that pups from aged fathers deviated from typical trajectories of vocal development, which were considered as atypical individuals. Thus, our study indicates that advanced paternal age has a significant effect on offspring’s early vocal development. It is suggested that the trajectories of vocal development could be a useful marker of the NDD-like phenotype associated with the advanced paternal age. In addition, our comprehensive computational analysis described here is an effective approach to characterize the altered individual diversity relevant to neurodevelopmental disorders.One Sentence SummaryAdvanced paternal age affects vocal development in early postnatal mice, with more pups showing atypical developmental trajectories.
The effect of habitat choice on evolutionary rescue in subdivided populations
Authors: Czuppon Peter , Blanquart François , Uecker Hildegard , Débarre Florence 
Published: August 2019

AbstractEvolutionary rescue is the process by which a population, in response to an environmental change, successfully avoids extinction through adaptation. In spatially structured environments, dispersal can affect the probability of rescue. Here, we model an environment consisting of patches that degrade one after another, and we investigate the probability of rescue by a mutant adapted to the degraded habitat. We focus on the effects of dispersal and of immigration biases. We find that the probability of evolutionary rescue can undergo up to three phases: (i) starting from low dispersal rates, it increases with dispersal; (ii) at intermediate dispersal rates, it decreases; (iii) finally, at large dispersal rates, the probability of rescue increases again with dispersal, except if mutants are too counter-selected in not-yet-degraded patches. The probability of rescue is generally highest when mutant and wild-type individuals preferentially immigrate into patches that have already undergone environmental change. Additionally, we find that mutants that will eventually rescue the population most likely first appear in non-degraded patches, and that the relative contribution of standing genetic variation vs. de-novo mutations declines with increasing emigration rates. Overall, our results show that habitat choice, when compared to the often studied unbiased immigration scheme, can substantially alter the dynamics of population survival and adaptation to new environments.
Developing a rapid and highly efficient cowpea regeneration and transformation system using embryonic axis explants
Authors: Che Ping , Chang Shujun , Simon Marissa K. , Zhang Zhifen , Shaharyar Ahmed , Ourada Jesse , O’Neill Dennis , Torres-Mendoza Mijael , Guo Yinping , Marasigan Kathleen M. , Vielle-Calzada Jean-Philippe , Ozias-Akins Peggy , Albertsen Marc C. , Jones Todd J. 
Published: August 2019

SummaryCowpea is one of the most important legume crops planted worldwide, especially in Sub-Saharan Africa and Asia. Despite decades of effort, genetic engineering of cowpea is still challenging due to inefficient in vitro shoot regeneration, Agrobacterium-mediated T-DNA delivery and transgenic selection. Here, we report a rapid and highly efficient cowpea transformation system using embryonic axis explants isolated from imbibed mature seeds. We found that removal of the shoot apical meristem by cutting through the middle of the epicotyl stimulated direct multiple shoot organogenesis from the cotyledonary node tissue. Furthermore, the application of a ternary transformation vector system using an optimized pVIR accessory plasmid provided high levels of Agrobacterium-mediated gene delivery. The utilization of spectinomycin as the selection agent enabled more efficient transgenic selection and plant recovery. Transgenic cowpea shoots developed exclusively from the cotyledonary nodes at high frequencies of 4.5 to 37% across a wide range of cowpea genotypes. We believe that the transformation principles established in this study could also be applied to other legumes to increase transformation efficiencies.
Aging induces aberrant state transition kinetics in murine muscle stem cells
Authors: Kimmel Jacob C. , Hwang Ara B. , Marshall Wallace F. , Brack Andrew S. 
Published: August 2019

AbstractMurine muscle stem cells (MuSCs) experience a transition from quiescence to activation that is required for regeneration, but it remains unclear if the transition states and rates of activation are uniform across cells, or how features of this process may change with age. Here, we use timelapse imaging and single cell RNA-seq to measure activation trajectories and rates in young and aged MuSCs. We find that the activation trajectory is conserved in aged cells, and develop effective machine learning classifiers for cell age. Using cell behavior analysis and RNA velocity, we find that activation kinetics are delayed in aged MuSCs, suggesting that changes in stem cell dynamics may contribute to impaired stem cell function with age. Intriguingly, we also find that stem cell activation appears to be a random walk like process, with frequent reversals, rather than a continuous, linear progression. These results support a view of the aged stem cell phenotype as a combination of differences in the location of stable cell states and differences in transition rates between them.Summary StatementWe find that aged muscle stem cells display delayed activation dynamics, but retain a youthful activation trajectory, suggesting that changes to cell state dynamics may contribute to aging pathology.
Shuttling of Dorsal by Cactus: mechanism and implications
Authors: Schloop Allison E. , Carrell-Noel Sophia , Reeves Gregory T. 
Published: August 2019

AbstractIn a developing animal, morphogen gradients act to pattern tissues into distinct domains of cell types. However, despite their prevalence in development, morphogen gradient formation is a matter of debate. In our recent publication, we showed that the Dorsal/NF-κB morphogen gradient, which patterns the DV axis of the early Drosophila embryo, is partially established by a mechanism of facilitated diffusion. This mechanism, also known as “shuttling,” occurs when a binding partner of the morphogen facilitates the diffusion of the morphogen, allowing it to accumulate at a given site. In this case, the inhibitor Cactus/IκB facilitates the diffusion of Dorsal/NF-κB. In the fly embryo, we used computation and experiment to not only show that shuttling occurs in the embryo, but also that it enables the viability of embryos that inherit only one copy ofdorsalmaternally. Here we further discuss our evidence behind the shuttling mechanism, the previous literature data explained by the mechanism, and how it may also be critical for robustness of development. Finally, we describe an interaction between Dorsal and BMP signaling that is likely affected by shuttling.
A probabilistic framework for cellular lineage reconstruction using single-cell 5-hydroxymethylcytosine sequencing
Authors: Wangsanuwat Chatarin , Aldeguer Javier F. , Rivron Nicolas C. , Dey Siddharth S. 
Published: August 2019

AbstractLineage reconstruction is central to understanding tissue development and maintenance. While powerful tools to infer cellular relationships have been developed, these methods typically have a clonal resolution that prevent the reconstruction of lineage trees at an individual cell division resolution. Moreover, these methods require a transgene, which poses a significant barrier in the study of human tissues. To overcome these limitations, we report scPECLR, a probabilistic algorithm to endogenously infer lineage trees at a single cell-division resolution using 5-hydroxymethylcytosine. When applied to 8-cell preimplantation mouse embryos, scPECLR predicts the full lineage tree with greater than 95% accuracy. Further, scPECLR can accurately extract lineage information for a majority of cells when reconstructing larger trees. Finally, we show that scPECLR can also be used to map chromosome strand segregation patterns during cell division, thereby providing a strategy to test the “immortal strand” hypothesis in stem cell biology. Thus, scPECLR provides a generalized method to endogenously reconstruct lineage trees at an individual cell-division resolution.
Identification and mapping of central pair proteins by proteomic analysis
Authors: Dai Daniel , Ichikawa Muneyoshi , Peri Katya , Rebinsky Reid , Bui Khanh Huy 
Published: August 2019

AbstractCilia or flagella of eukaryotes are small micro-hair like structures that are indispensable to single-cell motility and play an important role in mammalian biological processes. Cilia or flagella are composed of nine doublet microtubules surrounding a pair of singlet microtubules called the central pair (CP). Together, this arrangement forms the canonical and highly conserved 9+2 axonemal structure. The CP, which is a unique structure exclusive to motile cilia, is a pair of structurally dimorphic singlet microtubules decorated with numerous associated proteins. Mutations of CP-associated proteins cause several different physical symptoms termed ciliopathies. Thus, it is crucial to understand the architecture of the CP. However, the protein composition of the CP was poorly understood. This was because identification of CP proteins was mostly limited by availableChlamydomonasmutants of CP proteins. In this study, we conducted a comprehensive CP proteome analysis using several CP mutants and identified 37 novel CP protein candidates. By usingChlamydomonasstrains lacking specific CP sub-structures, we also present a more complete model of localization of known and newly identified CP proteins. This work has established a new foundation for CP protein analysis for future studies.
CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4
Authors: Garrett Andrew M. , Bosch Peter J. , Steffen David M. , Fuller Leah C. , Marcucci Charles G. , Koch Alexis A. , Bais Preeti , Weiner Joshua A. , Burgess Robert W. 
Published: August 2019

ABSTRACTThe mammalian Pcdhg gene cluster encodes a family of 22 cell adhesion molecules, the gamma-Protocadherins (γ-Pcdhs), critical for neuronal survival and neural circuit formation. The extent to which isoform diversity–aγ-Pcdh hallmark–is required for their functions remains unclear. We used a CRISPR/Cas9 approach to reduce isoform diversity, targeting each Pcdhg variable exon with pooled sgRNAs to generate an allelic series of 26 mouse lines with 1 to 21 isoforms disrupted via discrete indels at guide sites and/or larger deletions/rearrangements. Analysis of 5 mutant lines indicates that postnatal viability and neuronal survival do not require isoform diversity. Surprisingly, as it is the only γ-Pcdh that cannot independently engage in homophilic trans-interactions, we find that γC4, encoded by Pcdhgc4, is the only critical isoform. Because the human orthologue is the only PCDHG gene constrained in humans, our results indicate a conserved γC4 function that likely involves distinct molecular mechanisms.
TAL1-mediated epigenetic modifications down-regulate the expression of GIMAP family in non-small cell lung cancer
Authors: Tang Zhongxiang , Wang Lili , Dai Pei , Ruan Pinglang , Liu Dan , Tan Yurong 
Published: August 2019

AbstractBackgroudThe study was designed to explore the role of GIMAP family in non-small cell lung cancer (NSCLC) and its possible expression regulation mechanisms using existing biological databases including encyclopedia of DNA elements (ENCODE), gene expression omnibus (GEO), and the cancer genome atlas (TCGA).MethodsLung squamous cell carcinoma (LUSC) and lung adenocarcinoma (LUAD) were used to evaluate the expression of GIMAPs in TCGA. Five NSCLC datasets were then selected from GEO for validation. RNA-seq and Chip-seq data from ENCODE and GEO were used to observe epigenetic modifications on the chromosomes of GIAMPs in NSCLC. We constructed protein-protein interaction (PPI) network to reveal the main interacting proteins of GIMAPs. We then analyzed the correlation and regulatory mechanism between TAL1 and the expression of GIMAP family. We also used Kaplan-Meier for survival analysis.ResultsAll 7 genes in the GIMAP family were down-regulated in NSCLC. H3K4me3 and H3K27ac disappeared, while H3K27me3 increased on the chromosome of this family. The expression of TAL1 was positively correlated with the expression of GIMAPs. sh-TAL1 significantly down-regulated the expression of GIMAP family through epigenetic modification. High expression of GIMAPs and TAL1 was found to be associated with a good prognosis of NLCSC.ConclusionThe downregulation of TAL1 caused the disappearance of H3K27ac and H3K4me3. It also caused an increase in H3K27me3 on the GIMAPs gene, eventually leading to the overall downregulation of the GIMAP family genes.
Understanding online review helpfulness in omnichannel retailing
Authors: Yang Shuiqing , Zhou Yusheng , Yao Jianrong , Chen Yuangao , Wei June 
Published: August 2019

Purpose
As retailers have increasingly embraced an omnichannel retailing strategy, explaining and predicting the helpfulness of online review should consider both online-based and offline-based reviews. The paper aims to discuss this issue.

Design/methodology/approach
Based on the signaling theory, this study intends to examine the impacts of review-related and reviewer-related signals on review helpfulness in the context of omnichannel retailing. The proposed research model and corresponding hypotheses were tested by using negative binomial regression.

Findings
The results shown that both review-related (review rating and review sentiment strength) and reviewer-related (reviewer real name and reviewer expertise) signals positively affect review helpfulness. Contrary to the authors’ expectations, review length negatively affects review helpfulness. Specifically, when the review submitted from an omnichannel retailer’s offline channel, the positive impacts of reviewer real name on review helpfulness will be stronger, and the positive impacts of reviewer expertise on review helpfulness will be weaker.

Originality/value
Unlike many previous studies tend to explore the antecedents of review helpfulness in a single-channel setting, the study examined the factors that affect review helpfulness in an omnichannel retailing context.
Interfunctional coordination: the role of digitalization
Authors: Ruiz-Alba José L. , Guesalaga Rodrigo , Ayestarán Raquel , Morales Mediano Javier 
Published: August 2019

Purpose
This paper aims to investigate interfunctional coordination (IC) in a B2B context. More specifically, it explores the role of digitalization as a strategic driver for an effective IC.

Design/methodology/approach
Following a qualitative methodology, three studies have been integrated: Study 1 (focus group with 5 participants), Study 2 (31 in-depth interviews with top executives) and Study 3 (online focus group with 9 experts).

Findings
One finding is that digitalization is the main driver for IC and can be considered strategic. Other findings show that digitalization can enhance IC, but it was also found that digitalization can have negative side effects on IC.

Originality/value
This study contributes to the understanding of the importance of digitalization on IC and also contributes to the conceptualization of IC as a dynamic capability.
Status and sustainability
Authors: Hammad Hadeer , Muster Viola , El-Bassiouny Noha M. , Schaefer Martina 
Published: August 2019

Purpose
Conspicuous consumption and sustainable consumption are commonly understood as being in contradiction with each other. Yet, scholars have recently become increasingly interested in examining positive relationships between these forms of consumption. The purpose of this paper is to conceptualize the synergies and contradictions between sustainable and luxury consumption and proposing whether and how conspicuous motives can foster a shift towards sustainable consumption in newly industrialized countries in general and Egypt in particular.

Design/methodology/approach
This paper is a conceptual note, intended as a starting point and acting as an eye-opener regarding the values inherent in both conspicuous and sustainable consumption and the potential influence that conspicuous motivations could have on the latter.

Findings
The paper discusses the possibilities for and limitations of conspicuous motives to foster sustainable consumption in newly industrialized countries in the Middle East. The adoption of westernized lifestyles, spreading in Middle Eastern countries, can represent a venue for motivating sustainable consumption behaviours as a means of status distinction. On the other hand, the trickle-down effect and the preconditions of visibility and exclusiveness pose risks on promoting sustainable consumption by addressing conspicuous motives.

Practical implications
The paper suggests that the synergistic interplay between conspicuous and sustainable consumption, as well as barriers and motivations underpinning both constructs, needs to be empirically researched, while factoring in the cultural specifics of the countries under study, as cultural nuances can influence the dynamics of interaction between conspicuous and sustainable behaviours.

Originality/value
Given the salience of the relationship between luxury and sustainable consumption and the focus of most studies on early-industrialized countries, insights regarding the possible influences of conspicuous motives on sustainable consumption in newly industrialized countries are warranted. With the scarcity of research examining the ambiguous relationship between conspicuous and sustainable consumption in newly industrialized countries, this paper contributes by providing insights about the conditions that can help conspicuous motives promote sustainable consumption in newly industrialized countries.
A combination of cognitive training and physical exercise for elderly with the mild cognitive impairment
Authors: Untari Ida , Subijanto Achmad Arman , Mirawati Dyah Kurnia , Probandari Ari Natalia , Sanusi Rossi 
Published: August 2019

Purpose
The purpose of this paper is to conduct systematic reviews on Indonesian papers, to examine the most recent evidence of the efficacy of the combination of cognitive training and physical exercise, and to make recommendations in order to improve prevention, care and treatment services in elderly patients with mild cognitive impairment (MCI).

Design/methodology/approach
The databases of Cochrane, Medline, NIH (US National Library Medicine), ProQuest, EbscoHost, Clinical Key, EMBASE, Medical Librarian (TWE) in Ovid, Science Direct, Scopus, The Lancet Global Health, PubMed, Emerald, Indonesian National Library, Google Scholar, Google Indonesia, and Garuda Portal were systematically searched using Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines to obtain empirical papers published between June 1976 and January 2018.

Findings
Out of the 3,293 articles collected, 10 were included in this analysis. The result of this combined meta-analysis compares the combination therapy group (cognitive therapy and physical exercise) with a control group. It shows that the control group was likely to experience MCI 1.65 times more often than the combination therapy group. According to the result acquired from the synthesized meta-analysis, the control group experienced MCI 1.65 times higher than the combination therapy. The finding is proven to be statistically significant (95% CI= 1.42–1.93).

Research limitations/implications
The research considers only English and Indonesian articles.

Practical implications
It is important to explore the most effective training characteristics in a special combined intervention differentiated by the duration, frequency, intervention, type and combination mode. There is a need for further investigation that focuses on the physiological mechanisms underlying the positive effects, by inserting a more comprehensive neuro-imaging measurement to assess specifically the domain that benefits in terms of cognitive functions and molecular markers. Finally, exploratory studies are definitely required, which will specifically examine maintenance and treatment effects as well as derive theoretical explanations related to the interventions and predictors.

Social implications
A combination of cognitive training and physical exercise intervention may improve the global health or cognitive functions.

Originality/value
A combination of cognitive training and physical exercise has been found to improve prevention, care and treatment services in elderly patients with MCI. There is an increase in value in comparison to the study of Karssemeijer, which considered five Indonesian articles.
Olive cake reduces glycaemia and lipemia and increases antioxidant enzymes in STZ-induced diabetes in rat erythrocytes and tissues
Authors: Cherrad Hayet , Bouderbala Sherazede , Zidan Yahiaoui , Krouf Djamil 
Published: August 2019

PurposeThe purpose of this study is to determine the effect of olive cake (CO) on glycaemia and lipemia and lipid peroxidation and antioxidant enzymes activities in erythrocytes and tissues, in streptozotocin (STZ)-induced diabetic rats.Design/methodology/approachDiabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg BW). In total, 12 diabetic D rats, weighing 260 ± 20 g, were divided into two groups fed a casein diet supplemented (D-OC) or not (D) with OC (7.5 per cent), for four weeks.FindingsIn D-OC compared with D, glycaemia, total cholesterol and triglycerides values (−40 per cent;p = 0.007, 27 per cent;p = 0.007 and −27 per cent;p = 0.0019). In erythrocyte, liver, kidney, heart, muscle and brain, thiobarbituric acid reactive substances contents were respectively, (−19 per cent;p = 0.03, −32 per cent;p = 0.002, −20 per cent;p = 0.04, −68 per cent;p = 0.003, −74 per cent;p = 0.0003 and −38 per cent;p = 0.04). In erythrocyte, SOD, GSH-Px and CAT activities were respectively, (+14 per cent;p = 0.01, +74 per cent;p = 0.012 and +34 per cent;p = 0.0009). In the liver, kidney, heart and muscle, SOD activity was respectively, (+31 per cent;p= 0.004, +12 per cent;p = 0.038, +43 per cent;p = 0.001 and +23 per cent;p = 0.18). GSH-Px activity was respectively, (+121 per cent;p = 0.0009, 89 per cent;p = 0.0006, + 95 per cent;p= 0.008, +71 per cent;p = 0.02 and +26 per cent;p = 0.01), in the liver, kidney, heart, muscle and brain. Catalase activity was (+21 per cent;p = 0.008) in the liver, (+88 per cent;p = 0.0002 in the kidney, +53 per cent;p = 0,002 in the heart and 83 per cent;p = 0.00004 in the muscle).Originality/valueIn diabetic rats, OC reduces hyperglycaemia induced by STZ and attenuates triglyceridemia and cholesterolemia. This residue is able to decrease the oxidative stress by increasing the antioxidant enzymes activity in erythrocytes and tissues. The high contents of phytoconstituents present in OC are considered to be responsible for this effect.