bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative proteomics reveals key roles for post-transcriptional gene regulation in the molecular pathology of FSHD
Authors: Jagannathan Sujatha , Ogata Yuko , Gafken Philip R. , Tapscott Stephen J. , Bradley Robert K. 
Published: September 2018

AbstractDUX4 is a transcription factor whose misexpression in skeletal muscle causes facioscapulohumeral muscular dystrophy (FSHD). While DUX4’s transcriptional activity has been extensively characterized, the DUX4-induced proteome remains undescribed. Here, we report concurrent measurement of RNA and protein levels in DUX4-expressing cells via RNA-seq and quantitative mass spectrometry. DUX4 transcriptional targets were robustly translated, confirming the likely clinical relevance of proposed FSHD biomarkers. However, a multitude of mRNAs and proteins exhibited discordant expression changes upon DUX4 expression. Our dataset revealed unexpected proteomic, but not transcriptomic, dysregulation of diverse molecular pathways, including Golgi apparatus fragmentation, as well as extensive post-transcriptional buffering of stress response genes. Key components of RNA degradation machineries, including UPF1, UPF3B, and XRN1, exhibited suppressed protein, but not mRNA, levels, explaining the build-up of aberrant RNAs that characterizes DUX4-expressing cells. Our results provide a resource for the FSHD community and illustrate the importance of post-transcriptional processes to DUX4-induced pathology.
Phylogeny, evidence for a cryptic plastid, and distribution of Chytriodinium parasites (Dinophyceae) infecting copepods
Authors: Strassert Jürgen F. H. , Hehenberger Elisabeth , del Campo Javier , Okamoto Noriko , Kolisko Martin , Richards Thomas A. , Worden Alexandra Z. , Santoro Alyson E. , Keeling Patrick J. 
Published: September 2018

ABSTRACTSpores of the dinoflagellate Chytriodinium are known to infest copepod eggs causing their lethality. Despite the potential to control the population of such an ecologically important host, knowledge about Chytriodinium parasites is limited: we know little about phylogeny, parasitism, abundance, or geographical distribution. We carried out genome sequence surveys on four manually isolated sporocytes from the same sporangium to analyse the phylogenetic position of Chytriodinium based on SSU and concatenated SSU/LSU rRNA gene sequences, and also characterize two genes related to the plastidial heme pathway, hemL and hemY. The results suggest the presence of a cryptic plastid in Chytriodinium and a photosynthetic ancestral state of the parasitic Chytriodinium/Dissodinium clade. Finally, by mapping Tara Oceans V9 SSU amplicon data to the recovered SSU rRNA gene sequences from the sporocytes, we show that globally, Chytriodinium parasites are most abundant within the pico/nano- and mesoplankton of the surface ocean and almost absent within microplankton, a distribution indicating that they generally exist either as free-living spores or host-associated sporangia.
Reward-driven changes in striatal pathway competition shape evidence evaluation in decision-making
Authors: Dunovan Kyle , Vich Catalina , Clapp Matthew , Verstynen Timothy , Rubin Jonathan 
Published: September 2018

AbstractCortico-basal-ganglia-thalamic (CBGT) networks are critical for adaptive decision-making, yet how changes to circuit-level properties impact cognitive algorithms remains unclear. Here we explore how dopaminergic plasticity at corticostriatal synapses alters competition between striatal pathways, impacting the evidence accumulation process during decision-making. Spike-timing dependent plasticity simulations showed that dopaminergic feedback based on rewards modified the ratio of direct and indirect corticostriatal weights within opposing action channels. Using the learned weight ratios in a full spiking CBGT network model, we simulated neural dynamics and decision outcomes in a reward-driven decision task and fit them with a drift diffusion model. Fits revealed that the rate of evidence accumulation varied with inter-channel differences in direct pathway activity while boundary height varied with overall indirect pathway activity. This multi-level modeling approach demonstrates how complementary learning and decision computations can emerge from corticostriatal plasticity.Author summaryCognitive process models such as reinforcement learning (RL) and the drift diffusion model (DDM) have helped to elucidate the basic algorithms underlying error-corrective learning and the evaluation of accumulating decision evidence leading up to a choice. While these relatively abstract models help to guide experimental and theoretical probes into associated phenomena, they remain uninformative about the actual physical mechanics by which learning and decision algorithms are carried out in a neurobiological substrate during adaptive choice behavior. Here we present an “upwards mapping” approach to bridging neural and cognitive models of value-based decision-making, showing how dopaminergic feedback alters the network-level dynamics of cortico-basal-ganglia-thalamic (CBGT) pathways during learning to bias behavioral choice towards more rewarding actions. By mapping “up” the levels of analysis, this approach yields specific predictions about aspects of neuronal activity that map to the quantities appearing in the cognitive decision-making framework.
Inverse occlusion, a binocularly motivated treatment for amblyopia
Authors: Zhou Jiawei , Wu Yidong , Chen Yiya , Chen Xiaoxin , Liang Yunjie , Mao Yu , Yao Zhimo , He Zhifen , Lu Fan , Qu Jia , Hess Robert F. 
Published: September 2018

AbstractRecent laboratory finding suggest that short-term patching the amblyopic eye (i.e., inverse occlusion) results in a larger and more sustained improvement in the binocular balance compared with normal controls. In this study, we investigate the cumulative effects of the short-term inverse occlusion in adults and old children with amblyopia. A prospective cohort study of 18 amblyopes (10-35 years old; 3 with strabismus) who have been subjected to 2 hours/day of inverse occlusion for 2 months. Patients who required refractive correction or whose refractive correction needed updating were given a 2-month period of refractive adaptation. The primary outcome measure was the binocular balance which was measured using a phase combination task, the secondary outcome measures were the best corrected visual acuity which was measured with a Tumbling E acuity chart and convert to logMAR units and the stereo acuity which was measured with the Random-dot preschool stereotest. The averaged binocular gain was 0.11 in terms of the effective contrast ratio (z = −2.344, p = 0.019, 2-tailed Related samples Wilcoxon Signed Ranks Test). The average acuity gain was 0.14 logMAR equivalent (t(17) = 0.13, p < 0.001, 2-tailed paired samples t-test). The averaged stereo acuity gain was 253 arc seconds (z = −2.689, p = 0.007). Based on more recent research concerning adult ocular dominance plasticity, contrary to current practice, patching the amblyopic eye makes more sense; comparable acuity benefits, better compliance, better binocular outcome and applicable to adults as well as old children.
Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis
Authors: Castel Baptiste , Tomlinson Laurence , Locci Federica , Yang Ying , Jones Jonathan D G 
Published: September 2018

ABSTRACTBacterial CRISPR systems have been widely adopted to create operator-specified site-specific nucleases. Such nuclease action commonly results in loss-of-function alleles, facilitating functional analysis of genes and gene families We conducted a systematic comparison of components and T-DNA architectures for CRISPR-mediated gene editing in Arabidopsis, testing multiple promoters, terminators, sgRNA backbones and Cas9 alleles. We identified a T-DNA architecture that usually results in stable (i.e. homozygous) mutations in the first generation after transformation. Notably, the transcription of sgRNA and Cas9 in head-to-head divergent orientation usually resulted in highly active lines. Our Arabidopsis data may prove useful for optimization of CRISPR methods in other plants.
Distribution of duck-origin parvovirus over time in Cherry Valley ducks in vivo and histopathological investigation
Authors: Chen Bing , Luo Qihui , Xu Jing , Huang Chao , Liu Wentao , Cheng An-chun , Chen Zhengli 
Published: September 2018

AbstractIn 2015, we successfully isolated a strain of duck-origin parvovirus from Cherry Valley ducks, which we named QH-L01. In this study, duck-origin parvovirus in Cherry Valley ducks was quantified and localized by quantitative real-time PCR (qPCR) and immunohistochemistry (IHC), and pathological damage to the tissues and organs was observed by hematoxylin-eosin staining (HE staining). qPCR showed that the viral load was higher in the spleen, brain, lung, cecum, ileum, and duodenum over time. The results from IHC experiments showed positive reactions in hepatocytes, epithelium of the lung atrium, myocardial cells, goblet cells of the intestine, and brain cells. Primary histological examination revealed pulmonary lobule depletion and dilation in the lung as well as necrosis and erosion of the villus tips in the duodenum, ileum and cecum. This study is the first demonstration that duck-origin parvovirus can be transmitted from the spleen to the brain and lung, resulting in proliferation and dissemination of the virus to the cecum, ileum, duodenum and other tissues through the blood. The lung, duodenum, ileum and cecum may thus represent the main target tissues and organs for duck-origin parvovirus.
Quality cost of material procurement in construction projects
Authors: Sawan Rema , Low Jwen Fai , Schiffauerova Andrea 
Published: September 2018

Purpose
Material procurement constitutes a large share of the overall cost of construction projects. Understanding the factors influencing the cost of quality (COQ) in the procurement process could help identify opportunities for lowering quality cost without compromising quality. The paper aims to discuss these issues.

Design/methodology/approach
In this paper, a COQ model for the construction material procurement process is developed using the traditional prevention–appraisal–failure (PAF) approach. Using data from a $4bn aluminum smelter construction project, the authors conducted a simulation of the COQ model to evaluate various quality assurance policies.

Findings
This paper confirms that raising the prevention cost leads to a drop in failure cost as well as COQ for the project studied. While the authors are unable to provide blanket recommendations as the results are derived from a single project case study, it does suggest that construction material procurement processes would benefit from a higher prevention expenditure. And for certain cases where the authors observe a deviation from the traditional Juran’s model of COQ – the high appraisal cost in the procurement process – reduction of appraisal expenditure may in fact be more beneficial than its increase.

Originality/value
The research results suggest that appraisal expenditure should be tailored to each purchase order in order to maximize the total benefits. Additionally, this paper presents the first COQ model developed for the construction material procurement process. Another unique feature of the model is its inclusion of supplier-side costs, which are excluded in the conventional COQ analysis.
Socio-economic disparity in the occurrence of disability among older adults in six low and middle income countries
Authors: Rahman Mohammad Hifz Ur , Singh Ashish 
Published: September 2018

PurposeNearly 200m people in the world experience considerable functioning difficulties. Also, more than three-fourth of the population aged 50 years and over is suffering from some kind of disability in India, China, Ghana, Russia, Mexico and South Africa. Despite the compelling nature of this issue, evidence on socioeconomic disparity in the occurrence of disability is lacking throughout the world and particularly in the aforementioned countries. The purpose of this paper is twofold – first, to examine the socioeconomic inequalities in the prevalence of disability in the selected countries; and second, to investigate the cross-country differentials in the prevalence of disability by socioeconomic characteristics.Design/methodology/approachThe authors use data from the Study on Global Ageing and Adult Health (SAGE) conducted in China, Ghana, India, Mexico, Russia and South Africa during 2007–2010. Disability scores have been constructed using Item Response Theory Partial Credit Model based on eight health and functioning domains. Bivariate analysis, concentration curves, concentration indices and multivariate regressions have been used in the analysis presented in this paper.FindingsThe authors find that the prevalence of disability varied considerably across sociodemographic groups. Moreover, this variation is not uniform across all countries. Also, age, Sex, work status, years of schooling and economic status emerged out as significant predictors of disability among the studied countries.Originality/valueThis is perhaps the first study which examines the socioeconomic inequality in disability conceptualized in a comprehensive manner among older adults spread across low to upper middle income countries. The alarming level of prevalence of disability among sociodemographic disadvantage groups calls for immediate attention in terms of detailed study of risk factors, effective policy and timely intervention.
Decision modeling of risks in pharmaceutical supply chains
Authors: Moktadir Md. Abdul , Ali Syed Mithun , Mangla Sachin Kumar , Sharmy Tasnim Ahmed , Luthra Sunil , Mishra Nishikant , Garza-Reyes Jose Arturo 
Published: September 2018

PurposeManaging risks is becoming a highly focused activity in the health service sector. In particular, due to the complex nature of processes in the pharmaceutical industry, several risks have been associated to its supply chains. The purpose of this paper is to identify and analyze the risks occurring in the supply chains of the pharmaceutical industry and propose a decision model, based on the Analytical Hierarchy Process (AHP) method, for evaluating risks in pharmaceutical supply chains (PSCs).Design/methodology/approachThe proposed model was developed based on the Delphi method and AHP techniques. The Delphi method helped to select the relevant risks associated to PSCs. A total of 16 sub risks within four main risks were identified through an extensive review of the literature and by conducting a further investigation with experts from five pharmaceutical companies in Bangladesh. AHP contributed to the analysis of the risks and determination of their priorities.FindingsThe results of the study indicated that supply-related risks such as fluctuation in imports arrival, lack of information sharing, key supplier failure and non-availability of materials should be prioritized over operational, financial and demand-related risks.Originality/valueThis work is one of the initial contributions in the literature that focused on identifying and evaluating PSC risks in the context of Bangladesh. This research work can assist practitioners and industrial managers in the pharmaceutical industry in taking proactive action to minimize its supply chain risks. To the end, the authors performed a sensitivity analysis test, which gives an understanding of the stability of ranking of risks.
Mediating effect of intrinsic motivation on the relationship between Islamic work ethic, job satisfaction, and organizational commitment in banking sector
Authors: Gheitani Alborz , Imani Saheb , Seyyedamiri Nader , Foroudi Pantea 
Published: September 2018

PurposeIn line with the general purpose mentioned, this paper aims to determine the impact of the Islamic work ethic (IWE) on job satisfaction and organizational commitment among the employees of Bank Maskan by examining the mediating role of intrinsic motivation.Design/methodology/approachAnalysis of data obtained from 220 questionnaires related to research variables with AMOS software shows a positive and significant relationship between IWE and job satisfaction and organizational commitment with the mediating role of intrinsic motivation.FindingsThe findings revealed a direct effect of IWE on job satisfaction, but there was no direct significant relationship between this variable and organizational commitment. Also, intrinsic motivation plays a partial and completely mediatory role in the relationship between IWE and job satisfaction and between IWE and organizational commitment.Research limitations/implicationsThe impact of participation in strategic planning on managers’ creation of budgetary slack: The mediating role of autonomous motivation and affective organizational commitment.Originality/valueAs the nature of bank employees’ work is such that it confronts them with numerous ethical choices, the adherence to ethical standards, particularly IWE, can greatly affect their enthusiasm and, as a result, their satisfaction and organizational commitment.
Modelling the barriers to online banking in the Indian scenario: an ISM approach
Authors: Katiyar Rajesh , Badola Sneha 
Published: September 2018

Purpose
Online banking, a phenomenon widely adopted and appreciated in developing and developed countries, is becoming an essential part of the lives of people from all walks of life, but there exist a good fraction of customers resisting these services. Therefore, the purpose of this paper is to identify the barriers generating resistance to online banking. The special focus of the paper is to explore resistance among bank customers who already have valid contracts for online banking but still prefer to approach the traditional branches for transactions.

Design/methodology/approach
To achieve the objective, interpretive structural modelling (ISM) with cross-impact matrix multiplication applied to classification (MICMAC) approach is used to investigate the interrelationships among the barriers to online banking.

Findings
Online banking, although proven to be a glorious innovation, has still not been adopted by all, and hence, has not met all the expectations of banks and banking sector. The outcome shows that the online illiteracy and online unawareness are the most important barriers and these two are working as the root cause and demand strong attention on part of the banking sector which is working towards the inclusion of customers to online banking. These findings may help the Indian banking sector in identifying the crucial points that affect online banking system in India the most.

Originality/value
This research explored the interactions among the barriers to online banking in the Indian scenario and revealed significant results through the ISM-based technique.
Business model innovation and value-creation: the triadic way
Authors: Andreassen Tor Wallin , Lervik-Olsen Line , Snyder Hannah , Van Riel Allard C.R. , Sweeney Jillian C. , Van Vaerenbergh Yves 
Published: September 2018

PurposeBuilding on the multi-divisional business model (M-model), the purpose of this paper is to develop a better understanding of triadic business models – T-models – and how they create value for their three categories of stakeholders, i.e., the suppliers, the platform firm and the buyers. The research question that guides the present study is twofold: How is value created individually and collectively in triadic business models and what might challenge their sustainability?Design/methodology/approachAnchored in extant literature and a process of conceptual modeling with empirical examples from Uber, a new business model archetype was developed for two-sided markets mediated by a middleman.FindingsThe paper provides a theoretically and conceptually derived roadmap for sustainable business in a triadic business model, i.e., for the buyers, sellers and the platform firm. This model is coined the T-model. A number of propositions are derived that argue the relationship between key constructs. Finally, the future beyond the T-model is explored.Research limitations/implicationsThe paper identifies, illustrates and discusses the ways in which value is created in sustainable T-models. First, value is created from a number of sources, not only from lower transaction costs. Second, it is proposed that it is not about a choice of either M-model or T-model but rather a continuum. Toward 2050, technology in general and Blockchain specifically may for some transactions or services, eliminate the need for middlemen. The main conclusion is that despite this development, there will, for most organizations, be elements of the M-model in all or most T-model businesses. In short: middlemen will have elements of the M-model embedded in the T-model when co creating value with buyers and sellers.Originality/valueWhile two-sided T-models are not new to the business area, surprisingly no papers have systematically investigated, illustrated, and discussed how value is created among and between the three stakeholder categories of the T-model. With this insight, more sustainable T-models can be created.
Mindfulness‐based stress reduction for menopausal symptoms after risk‐reducing salpingo‐oophorectomy (PURSUE study): a randomised controlled trial
Authors: van Driel CMG , de Bock GH , Schroevers MJ , Mourits MJ 
Published: September 2018

ObjectiveTo assess the short‐ and long‐term effects of mindfulness‐based stress reduction (MBSR) on the resulting quality of life, sexual functioning, and sexual distress after risk‐reducing salpingo‐oophorectomy (RRSO).DesignRandomised controlled trial.SettingA specialised family cancer clinic of the university medical center Groningen.PopulationSixty‐six women carriers of the BRCA1/2 mutation who developed at least two moderate‐to‐severe menopausal symptoms after RRSO.MethodsWomen were randomised to an 8‐week MBSR training programme or to care as usual (CAU).Main outcome measuresChange in the Menopause‐Specific Quality of Life Questionnaire (MENQOL), the Female Sexual Function Index, and the Female Sexual Distress Scale, administered from baseline at 3, 6, and 12 months. Linear mixed modelling was applied to compare the effect of MBSR with CAU over time.ResultsAt 3 and 12 months, there were statistically significant improvements in the MENQOL for the MBSR group compared with the CAU group (both P = 0.04). At 3 months, the mean MENQOL scores were 3.5 (95% confidence interval, 95% CI 3.0–3.9) and 3.8 (95% CI 3.3–4.2) for the MBSR and CAU groups, respectively; at 12 months, the corresponding values were 3.6 (95% CI 3.1–4.0) and 3.9 (95% CI 3.5–4.4). No significant differences were found between the MBSR and CAU groups in the other scores.ConclusionMindfulness‐based stress reduction was effective at improving quality of life in the short‐ and long‐term for patients with menopausal symptoms after RRSO; however, it was not associated with an improvement in sexual functioning or distress.Tweetable abstractMindfulness improves menopause‐related quality of life in women after risk‐reducing salpingo‐oophorectomy.