bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA GEHT1 promoted the proliferation of ovarian cancer cells via modulating the protein stability of HIF1α
Authors: Liu Dan , Li Hao 
Published: April 2019

Abstract
Cancer cells preferentially metabolize glucose via the aerobic glycolysis pathway, which is also named as Warburg effect. Increasing evidence has suggested that suppression of glycolysis inhibits the progression of cancers. In the present study, we found that the long non-coding RNA gastric carcinoma high expressed transcript 1 (GHET1) was overexpressed in ovarian cancer tissues and cell lines. Up-regulation of GHET1 was positively correlated with the tumor size and metastasis of the ovarian cancer patients. Overexpression of GEHT1 significantly promoted the proliferation and colony formation of ovarian cancer cells. Mechanistically, the candidate binding partners of GHET1 were explored by pull-down and mass spectrum. Of note, GHET1 was found to interact with the E3 ubiquitin ligase von Hippel-Lindau (VHL), which consequently blocked VHL-mediated degradation of hypoxia-inducible factor-1α (HIF1α) and enhanced the protein level of HIF1α in ovarian cancer cells. The up-regulated HIF1α promoted the glucose uptake and lactate generation of ovarian cancer cells. Collectively, our results suggested the oncogenic function of GHET1 via up-regulating the glycolysis in ovarian cancer and can be considered as a promising anti-cancer target.
Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Streptococcus agalactiae
Authors: Guo Xu-Guang , Zhuang Ya-Ru , Wen Jin-Zhou , Xie Tian-Ao , Liu Ye-Ling , Zhu Guo-Dong , Xia Yong 
Published: April 2019

Abstract
Streptococcus agalactiae is a major pathogenic bacterium causing perinatal infections in humans. In the present study, a novel real-time fluorescence loop-mediated isothermal amplification technology was successfully developed and evaluated for the detection of S. agalactiae in a single reaction. Six specific primers were designed to amplify the corresponding six regions of fbs B gene of S. agalactiae, using Bst DNA polymerase with DNA strand displacement activity at a constant temperature for 60 min. The presence of S. agalactiae was indicated by the fluorescence in real-time. Amplification of the targeted gene fragment was optimized with the primer 1 in the current setup. Positive result was only obtained for Sa by Real-LAMP among 10 tested relevant bacterial strains, with the detection sensitivity of 300 pg/µl. Real-LAMP was demonstrated to be a simple and rapid detection tool for S. agalactiae with high specificity and stability, which ensures its wide application and broad prospective utilization in clinical practice for the rapid detection of S. agalactiae.
Pharmacological inhibition of TRPV4 channels protects against ischemia–reperfusion-induced renal insufficiency in neonatal pigs
Authors: Soni Hitesh , Peixoto-Neves Dieniffer , Olushoga Michael A. , Adebiyi Adebowale 
Published: April 2019

AbstractRenal vasoconstriction, an early manifestation of ischemic acute kidney injury (AKI), results in renal hypoperfusion and a rapid decline in kidney function. The pathophysiological mechanisms that underlie ischemia–reperfusion (IR)-induced renal insufficiency are poorly understood, but possibilities include alterations in ion channel-dependent renal vasoregulation. In the present study, we show that pharmacological activation of TRPV4 channels constricted preglomerular microvessels and elicited renal hypoperfusion in neonatal pigs. Bilateral renal ischemia followed by short-term reperfusion increased TRPV4 protein expression in resistance size renal vessels and TRPV4-dependent cation currents in renal vascular smooth muscle cells (SMCs). Selective TRPV4 channel blockers attenuated IR-induced reduction in total renal blood flow (RBF), cortical perfusion, and glomerular filtration rate (GFR). TRPV4 inhibition also diminished renal IR-induced increase in AKI biomarkers. Furthermore, the level of angiotensin II (Ang II) was higher in the urine of IR- compared with sham-operated neonatal pigs. IR did not alter renal vascular expression of Ang II type 1 (AT1) receptors. However, losartan, a selective AT1 receptor antagonist, ameliorated IR-induced renal insufficiency in the pigs. Blockade of TRPV4 channels attenuated Ang II-evoked receptor-operated Ca2+ entry and constriction in preglomerular microvessels. TRPV4 inhibition also blunted Ang II-induced increase in renal vascular resistance (RVR) and hypoperfusion in the pigs. Together, our data suggest that SMC TRPV4-mediated renal vasoconstriction and the ensuing increase in RVR contribute to early hypoperfusion and renal insufficiency elicited by renal IR in neonatal pigs. We propose that multimodal signaling by renal vascular SMC TRPV4 channels controls neonatal renal microcirculation in health and disease.
Virushepatitis – wann daran denken?
Authors: Luxenburger Hendrik , Thimme Robert , Neumann-Haefelin Christoph 
Published: April 2019

AbstractChronic viral hepatitis can remain unrecognized but may nevertheless lead to liver cirrhosis and hepatocellular carcinoma. Thus, patients with elevated liver enzymes as well as risk groups need to be screened and treated for viral hepatitis. These groups include, in particular, migrants from countries with high HBV or HCV prevalence, persons with previous or current intravenous drug use, and homosexual men. For HBV- or HCV-associated diseases, such as panarteriitis nodosa, cryoglobulinemic vasculitis or B-cell lymphoma, antiviral therapy may lead to remission. Prior to high-dose immunosuppressive therapy, especially with regimes containing rituximab, chronic or resolved HBV infection must be ruled out or antiviral prophylaxis may be required to avoid a potentially fatal HBV reactivation.
Perkutane dilatative Tracheotomie – Schritt für Schritt
Authors: Merten Michaela , Sattler Peter , Karagiannidis Christian 
Published: April 2019

AbstractBedside percutaneous dilatational tracheostomy has become one of the most commonly used interventions in ICU medicine. Different techniques have been developed, but guidance of percutaneous dilatational tracheostomy by video bronchoscope has been suggested to be clinically reasonable for direct visualization. The current Step-by-Step tutorial gives a detailed instruction of the procedure with visualization of every single step offering tips and pitfalls of the procedure.
Aszites und erhöhte Nierenretentionsparameter bei einer 25-jährigen Frau
Authors: Löbermann Micha , Witte Maria , Nowak Holger , Reisinger Emil 
Published: April 2019

Zusammenfassung
Anamnese und klinischer Befund 25-jährige Patientin mit akuten dysurischen Beschwerden, 3 Monate zuvor war eine komplikationslose Spontangeburt erfolgt.
Untersuchungen Es zeigte sich ein zunehmender Aszites und laborchemische Zeichen eines akuten Nierenversagens.
Diagnose Hohe Kreatininwerte im Aszites ließen auf eine Fistelung von Urin in den Peritonealraum schließen.
Therapie und Verlauf Im Rahmen einer explorativen Laparotomie konnte eine kleine Blasenläsion geschlossen werden.
Folgerungen Spontane Rupturen der Harnblase sind selten und können sich klinisch als Aszites mit laborchemischen Hinweisen für ein akutes Nierenversagen darstellen. Die Ursachenabklärung kann sich schwierig gestalten.
Die primäre chologene Diarrhö in einer gastroenterologischen Praxis
Authors: Wenzel Henrich 
Published: April 2019

ZusammenfassungDie primäre chologene Diarrhö (PCD) wurde bei ihrer Erstbeschreibung 1976 als sehr seltene Erkrankung angesehen, heute wird die Inzidenz auf > 1 % geschätzt. Das diagnostische Instrumentarium ist unterschiedlich verfügbar und es gibt in Deutschland keine einheitliche Empfehlung zur Diagnostik. Die PCD wird immer noch zu selten diagnostiziert.Seit Anfang der 90er Jahre haben wir die Probebehandlung mit Colestyramin und anschließende Bestätigung durch den Gallensäureresorptionstest (SeHCAT-Test) mit einer 7-Tage-Retention von 20 % als Schwellenwert zum Nachweis der chologenen Diarrhö (CD) in unser diagnostisches Vorgehen aufgenommen.Ausgewertet werden konnten 70 Patienten mit positiver Probebehandlung zwischen April 1991 und März 2017, von denen 60 mit SeHCAT-Test untersucht wurden. Nicht aufgenommen wurden Patienten mit CD Typ 1 oder Typ 3, ausgenommen Z. n. Cholezystektomie.85 % (35/41) der Patienten mit PCD blieben bei einer Nachbeobachtungszeit von median 8,3 (1 – 23,6) Jahren behandlungsbedürftig, davon nahmen 68,6 % (24/35) Colestyramin, 31,4 % (11/35) andere Antidiarrhoika. 14,6 % (6/41) berichteten eine Spontanremission nach median 2,9 (0,7 – 5,7) Jahren.In der Gruppe von Patienten mit CD nach Cholezystektomie waren 82 % (8/11) nach median 7,7 (1 – 24,5) Jahren weiter behandlungsbedürftig, 8 nahmen Colestyramin, ein Patient keine Medikamente, zwei Spontanremissionen.Alle acht Patienten mit normalem SeHCAT-Test (7-Tage-Retention > 20 %) wurden nach median 3,6 (1,2 – 6,3) Monaten wieder beschwerdefrei.Auch 70 % (7/10) der Patienten ohne Bestätigung durch den SeHCAT-Test waren nach median 4,3 (3,7 – 18,3) Jahren weiter behandlungsbedürftig.Mit der Probebehandlung allein steht die Diagnose einer CD auf schwachen Füßen. Sie ist aber ubiquitär verfügbar und sollte im diagnostischen Algorithmus der chronischen Diarrhö einen festen Platz haben, wenn andere Verfahren nicht zur Verfügung stehen. Durch den primären Einsatz des SeHCAT-Tests ist nach aktueller Literatur eine deutlich höhere diagnostische Ausbeute zu erwarten und eine Einsparung unnötiger Folgeuntersuchungen möglich. Die therapeutischen Konsequenzen sind wegen der bekannten Limitationen des Colestyramin jedoch begrenzt. Eine besser verträgliche und zugelassene Alternative für Colestyramin wird dringend benötigt.
Wie wir stationäre Routinedaten im Rahmen der
Versorgungsforschung nutzen können
Authors: Baierlein Jochen , Leibinger Philipp , Zacharowski Kai , Meybohm Patrick 
Published: April 2019

Zusammenfassung
Zielsetzung Anhand der Daten des Statistischen Bundesamtes und den
Qualitätsberichten der Krankenhäuser liegen prinzipiell große Datenmengen vor,
die die stationäre Versorgungssituation beschreiben. Es soll aufgezeigt werden,
welche analytischen Möglichkeiten anhand dieser Datensätze gegeben sind und
welche Vor- und Nachteile mit diesen einhergehen.
Methodik Die Datensätze wurden von den zuständigen Institutionen für die
Jahre 2007–2016 (DRG-Statistik) bzw. für die Jahre 2012–2016 (Qualitätsberichte)
bezogen und aufbereitet, um diese anschließend anhand verschiedener Kriterien zu
beschreiben und zu beurteilen. Des Weiteren wurden anhand zweier klinischer
Auswertungsbeispiele (OPS 8–918: Multimodale Schmerztherapie und OPS 8–800:
Transfusion von Vollblut, Erythrozytenkonzentrat und Thrombozytenkonzentrat)
verschiedene Auswertungen vorgenommen, um Analysepotentiale und Limitationen
aufzuzeigen.
Ergebnis Anhand der Qualitätsberichte und der Daten des Statistischen
Bundesamtes können sowohl die Leistungsentwicklungen der Krankenhäuser als auch
die Fallzahlentwicklungen nach Patientenherkunft auf regionaler Ebene (Stadt-
und Landkreisebene) dargestellt werden. So zeigt sich bei der Multimodalen
Schmerztherapie ein Fallzahlzuwachs zwischen 2012 und 2016 von 45 %, bei den
Transfusionen hingegen kann zwischen 2013 und 2016 ein jährlicher Rückgang von
3,5 % gezeigt werden. Durch Überlagerung der beiden Datensätze können zudem
Versorgungslücken aufgezeigt werden, d. h. Gebiete identifiziert werden, in
denen Leistungen benötigt aber nicht angeboten werden.
Schlussfolgerung Bei beiden Datensätzen handelt es sich nahezu um
Vollerhebungen, die sich aus analytischer Sicht gut ergänzen und für
Auswertungen zusammengeführt werden können. Es ist möglich, die Versorgungslage
verschiedener Indikationen transparent darzustellen, deren Entwicklung
aufzuzeigen und Hinweise zu Optimierungspotentialen zu geben. Mit diesen kann
die Versorgungssituation bestimmter stationärer Leistungen sowohl angebotsseitig
(Krankenhäuser) als auch nachfrageseitig (Patienten) transparent dargestellt
werden.
Colitis ulcerosa: Kann eine Modulation der intestinalen Mikrobiota eine langfristige Remission bedingen?
Authors: Stallmach Andreas , Grunert Philip , Pieper Dietmar , Steube Arndt 
Published: April 2019

ZusammenfassungDas Verständnis der Colitis ulcerosa (CU) ist in den letzten Dekaden stetig gewachsen; unterschiedliche Therapiekonzepte sind für verschiedene Krankheitssituationen standardisiert. In der Pathogenese wird der gastrointestinalen Mikrobiota, Umweltfaktoren, überschießenden immunologischen Reaktionen und genetischen Faktoren eine immer größere Bedeutung zugeordnet. So zeigen zahlreiche klinische Beobachtungen eine enge Verbindung zwischen einer dysbiotischen Mikrobiota und der Erstmanifestation und dem Verlauf der CU an. Vor dem Hintergrund, dass genetische Faktoren und inflammatorische Reaktionen die Mikrobiota selber verändern können ist aber nicht klar, was Ursache und Folge ist. Der Fäkale Mikrobiom Transfer (FMT) ist der drastischste Eingriff um eine dysbiotische Mikrobiota zu normalisieren. Mittlerweile ist ein FMT eine akzeptierte Behandlung der rezidivierenden Clostridioides difficile-Infektion; zahlreiche Fallserien und kontrollierte Studien untersuchen dieses Konzept auch bei Patienten mit CU. Vor diesem Hintergrund erfolgt eine Zusammenfassung der aktuellen Literatur bezüglich Effektivität und Sicherheit. Mögliche Limitationen und offene Fragen werden diskutiert, um das Konzept des FMT zur Therapie der CU zu verbessern. Ohne Zweifel sind dringend weitere kontrollierte Studien notwendig; ein FMT sollte bei CU nicht außerhalb dieser erfolgen.
Reduced Mannose-Binding Lectin-Associated Serine Protease (MASP)-1 is Associated with Disturbed Coagulation in Septic Shock
Authors: Larsen Julie Brogaard , Laursen Mathies Appel , Hvas Christine Lodberg , Larsen Kim Michael , Thiel Steffen , Hvas Anne-Mette 
Published: April 2019

Background Activation of the complement system is part of the dysregulated immune response in sepsis. The mannose-binding lectin-associated serine proteases (MASP)-1 and -2 activate the lectin pathway of the complement system. Besides, these proteins can activate coagulation in vitro. However, the role of the lectin pathway proteins in the development of sepsis-related disseminated intravascular coagulation (DIC) is only sparsely investigated.
Aim This article investigates the association between lectin pathway proteins and coagulation disturbances in septic shock patients.
Materials and Methods We included 36 septic shock patients from the intensive care unit, Aarhus University Hospital, Denmark. Blood samples were obtained within 24 hours after admission (day 1), and subsequently on day 2 and day 3. Plasma concentrations of mannose-binding lectin (MBL), H-ficolin, M-ficolin, CL-L1, CL-K1, MASP-1, -2 and -3, MBL-associated proteins of 19 and 44 kDa as well as complement factor C3dg were assessed. Standard coagulation parameters, thrombin generation, thrombin–anti-thrombin (TAT) complex and pro-thrombin fragment 1 + 2 were measured. Sequential Organ Failure Assessment (SOFA) score, DIC score and 30-day mortality were assessed.
Results Reduced MASP-1 plasma concentration was associated with DIC score ≥5 (p = 0.02), impaired thrombin generation (p = 0.03) and lower plasma TAT complex levels (p = 0.03). No association was found between lectin pathway proteins and SOFA score or 30-day mortality.
Conclusion Reduced MASP-1 concentrations were associated with impaired coagulation in septic shock patients. This indicates that increased MASP-1 activation and consumption is associated with the more severe coagulation disturbances in sepsis and points to a possible role for MASP-1 in sepsis-related DIC.
Phraseology in teenage language in Spanish, English and Norwegian
Authors: Jørgensen Annette Myre , Olza Inés 
Published: April 2019

Abstract
A set of phraseological units that convey disagreement in Spanish, English and Norwegian teenage language are
observed from three perspectives in this paper: the phraseological, the pragmatic-discursive and the contrastive perspective. The
underlying assumption of the analysis is that the expression of disagreement in these terms among young people takes on certain
pragmatic nuances. The Madrid Oral Corpus of Teenage Talk (COLAm), the COLT-corpus (Corpus of London Teenage Talk) and the
UNO-corpus of Young Norwegian speakers (Ungdomsspråk i Norden, Oslo) enable a comparison of the use of these phraseological units
expressing disagreement among teenagers across these three languages.
Comparative Study of Tiger Identification Using Template Matching Approach based on Edge Patterns
Authors: Hariyanto E , Iqbal M , Siahaan A P U , Saragih K S , Batubara S 
Published: April 2019

Abstract
The presence of tigers in residential areas as predators was a threat to humans and their livestock. Through digital image processing, these animals could be identified by matching line patterns on the skin as a characteristic of the tiger using the template matching method, namely comparing the similarities between the lines pattern in the reference image with the main image. However, the presence of plant leaves and shrubs with various shapes could disguise tiger skin line patterns so that it could affect the accuracy of identification results. In this paper, we conducted a comparative study to identify tigers based on edge lines which were then processed using three edge detection method namely Canny, Laplacian and Sobel. The results of each operator were further processed using template matching algorithm to get the accuracy of object identification (tiger).
GSE spectra in uni-directional quantum systems
Authors: Akila Maram , Gutkin Boris 
Published: April 2019

Abstract
Generically, spectral statistics of spinless systems with time reversal invariance (TRI) and chaotic dynamics are well-described by the Gaussian orthogonal ensemble (GOE). However, if an additional symmetry is present, the spectrum can be split into independent sectors which statistics depend on the type of the group’s irreducible representation. In particular, this allows for the construction of TRI quantum graphs with spectral statistics characteristic of the Gaussian symplectic ensembles (GSE). To this end one usually has to use groups admitting pseudo-real irreducible representations. In this paper we show how GSE spectral statistics can be realized in TRI systems with simpler symmetry groups lacking pseudo-real representations. As an application, we provide a class of quantum graphs with only C
4 rotational symmetry possessing GSE spectral statistics.
Is the Bombali virus pathogenic in humans?
Authors: Martell Henry J , Masterson Stuart G , McGreig Jake E , Michaelis Martin , Wass Mark N 
Published: April 2019

Abstract

Motivation
The potential of the Bombali virus, a novel Ebolavirus, to cause disease in humans remains unknown. We have previously identified potential determinants of Ebolavirus pathogenicity in humans by analysing the amino acid positions that are differentially conserved (specificity determining positions; SDPs) between human pathogenic Ebolaviruses and the non-pathogenic Reston virus. Here, we include the many Ebolavirus genome sequences that have since become available into our analysis and investigate the amino acid sequence of the Bombali virus proteins at the SDPs that discriminate between human pathogenic and non-human pathogenic Ebolaviruses.

Results
The use of 1408 Ebolavirus genomes (196 in the original analysis) resulted in a set of 166 SDPs (reduced from 180), 146 (88%) of which were retained from the original analysis. This indicates the robustness of our approach and refines the set of SDPs that distinguish human pathogenic Ebolaviruses from Reston virus. At SDPs, Bombali virus shared the majority of amino acids with the human pathogenic Ebolaviruses (63.25%). However, for two SDPs in VP24 (M136L, R139S) that have been proposed to be critical for the lack of Reston virus human pathogenicity because they alter the VP24-karyopherin interaction, the Bombali virus amino acids match those of Reston virus. Thus, Bombali virus may not be pathogenic in humans. Supporting this, no Bombali virus-associated disease outbreaks have been reported, although Bombali virus was isolated from fruit bats cohabitating in close contact with humans, and anti-Ebolavirus antibodies that may indicate contact with Bombali virus have been detected in humans.

Availability and implementation
Data files are available from https://github.com/wasslab/EbolavirusSDPsBioinformatics2019.

Supplementary information
Supplementary data are available at Bioinformatics online.
Non-steroidal anti-inflammatory drugs in chronic kidney disease: a systematic review of prescription practices and use in primary care
Authors: Lefebvre Claire , Hindié Jade , Zappitelli Michael , Platt Robert W , Filion Kristian B 
Published: April 2019

Abstract

Background
Chronic kidney disease (CKD) management focuses on limiting further renal injury, including avoiding nephrotoxic medications such as non-steroidal anti-inflammatory drugs (NSAIDs). We performed a systematic review to evaluate the prevalence of primary care NSAID prescribing in this population.

Methods
We systematically searched MEDLINE and Embase from inception to October 2017 for observational studies examining NSAID prescribing practices or use in CKD patients in a primary care setting. The methodological quality of included studies was assessed independently by two authors using a modified version of the Agency for Healthcare Research and Quality’s Methodological Evaluation of Observational Research checklist.

Results
Our search generated 8055 potentially relevant publications, 304 of which were retrieved for full-text review. A total of 14 studies from 13 publications met our inclusion criteria. There were eight cohort and three cross-sectional studies, two quality improvement intervention studies and one prospective survey, representing a total of 49 209 CKD patients. Cross-sectional point prevalence of NSAID use in CKD patients ranged from 8 to 21%. Annual period prevalence rates ranged from 3 to 33%. Meta-analysis was not performed due to important clinical heterogeneity across study populations.

Conclusions
Evidence suggests that NSAID prescriptions/use in primary care among patients with CKD is variable and relatively high. Future research should explore reasons for this to better focus knowledge translation interventions aimed at reducing NSAID use in this patient population.