bluuebunny/crossref_metadata_2025_split huggingface dataset:
Behaviours of Sea Turtles in Shipwrecks in Northeast Brazil
Authors: da Silva Leitão Alana Thaís Teixeira , de Oliveira Alves Maria Danise , dos Santos José Carlos Pacheco , Bezerra Bruna 
Published: November 2024

ABSTRACT

Shipwrecks are one of the most common types of artificial reefs. They are home to several sea creatures, including sea turtles. Here, we aim to understand the relationship between sea turtles and shipwrecks by investigating species occurrence, behaviours and food availability in artificial reefs in Pernambuco, Northeast Brazil.

To map sea turtle occurrence in shipwrecks, we considered data from the literature, and we inspected 54,145 photos resulting from 867 recreational dives performed in 19 shipwrecks. We then selected four wrecks for systematic behavioural observations through 97 h and 56 min of passive video recordings. We also investigated potential food resources for turtles in these shipwrecks.

We identified three turtle species (i.e., Chelonia mydas, Eretmochelys imbricata and Caretta caretta), but most records were juvenile Chelonia mydas individuals using conserved wrecks. We recorded nine behaviours. ‘Resting’ was the predominant activity for all three species, representing over 60% of the records for all species. Their overall activity pattern varied. The wrecks were predominantly encrusted with ascidia, octocoral, sponge and algae—organisms that are part of sea turtles' diet. We highlight the ecological role of the shipwrecks for sea turtles since they may serve as shelter (especially for the juveniles of Chelonia mydas), potentially protecting them against predators and strong currents. The wrecks may also serve as potential feeding areas for sea turtles. We reinforce the need to establish appropriate regulations for recreational dive tourism in shipwrecks in Brazil to avoid disturbing sea turtles in this ecologically important artificial reef.
Utilization of Tin(IV) Complex of N‐Confused Porphyrin for Antiproliferative Activity and Antimicrobial Photodynamic Chemotherapy
Authors: Kumar Rajesh , Kumar Chaudhary Pankaj , Prasad Ramasare , Sankar Muniappan 
Published: November 2024

AbstractSn(IV) complex of N‐Confused Porphyrin (Sn(IV)‐NCP) has been prepared and characterized by several spectroscopic techniques to verify its structure and purity. Sn(IV)‐NCP shows a red shift in both the Soret and Q bands compared to the free base NCTPP. The last Q band appears in the NIR region. Based on these characteristics, we investigated the antiproliferative properties and antimicrobial photodynamic therapy (a‐PDT) efficiency of Sn(IV)‐NCP against the bacteria Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Further, we investigated the photodynamic activity of Sn (IV)‐NCP against Michigan cancer foundation‐7 (MCF‐7) cancer cells, to assess its potential as an effective therapeutic agent. Treated MCF‐7 cells with the compound show cytotoxic effects as compared to the untreated ones. At a higher concentration (128 μg/ml), Sn(IV)‐NCP exhibited 90 % inhibition, while at a lower concentration (32 μg/ml), it showed 70 % inhibition in MCF‐7 cells. The IC50 value for this compound against MCF‐7 cells was found 16.67 μg/ml. At 32 μg/ml, Sn(IV)‐NCP showed only around 4 % cell inhibition, indicating minimal cytotoxic effects on human embryonic kidney cells (HEK293).
Drinking water buffer intensity simulator (BIS): Development and practical simulations
Authors: Wahman David G. , Schock Michael R. , Lytle Darren A. 
Published: November 2024

AbstractAn established body of research over many decades has identified the importance of both bulk‐water and pipe scale surface microenvironment buffering to meet distribution system pH targets and reduce corrosivity toward metallic piping and components. Buffer intensity quantifies the ability of water to resist pH changes, and the greater the buffer intensity, the more resistant the water is to pH changes. To provide a practical tool for exploring buffer intensity, a buffer intensity simulator (BIS) was implemented in open‐source R code, incorporating typical chemical species (e.g., carbonate and orthophosphate) that contribute to drinking water buffer intensity along with temperature and ionic strength impacts. The BIS was verified against a parallel spreadsheet implementation and is publicly available at https://github.com/USEPA/BIS. Simulations were conducted to illustrate impacts related to buffer intensity using three practical scenarios: carbonate buffering in drinking waters, temperature impacts, and free ammonia presence from chloramine use and/or source water presence.
Strong Red Luminescence in Europium Complexes Solution for Anti‐Counterfeiting Applications
Authors: Yang Xue , Gao Dangli , Yu Jia , Zhang Xiangyu , Pang Qing , Chai Ruipeng , Yun Sining 
Published: November 2024

ABSTRACTEu3+‐activated phosphors with distinct photoluminescence properties are well‐suited for diverse applications, including lighting, sensing, and imaging. Despite their potential, the large‐scale and energy‐efficient production of Eu3+‐doped phosphors remains a significant challenge for industrial applications. This research delves into the luminescent performance of Eu3+ ions in nitrate solutions at room temperature by employing detailed spectroscopic characterization. Results reveal vibrant red luminescence at 594 nm and 616 nm in europium nitrate solutions, irrespective of concentration or solvent. We proposed a luminescent mechanism based on the formation of coordination complexes in nitrate solution. Furthermore, the investigation highlights the superior performance of NH2‐ over CH2‐ligands in mitigating the deactivation of Eu3+ emissive state induced by OH− oscillators in H2O solvent, leading to enhanced photoluminescence. Particularly, europium nitrate solutions without lengthy preparation exhibit ligand‐dependent luminescent feature, showcasing potential applications in anti‐counterfeiting and coordination group structure detection. This study here not only enhances our understanding of rare earth luminescence mechanisms, but also broadens the range of rare earth luminescent materials.
Perfusion Process Intensification for Lentivirus Production Using a Novel Scale‐Down Model
Authors: Klimpel Maximilian , Pflüger‐Müller Beatrice , Cascallana Marta Arrizabalaga , Schwingal Sarah , Lal Nikki Indresh , Noll Thomas , Pirzas Vicky , Laux Holger 
Published: November 2024

ABSTRACTProcess intensification has become an important strategy to lower production costs and increase manufacturing capacities for biopharmaceutical products. In particular for the production of viral vectors like lentiviruses (LVs), the transition from (fed‐)batch to perfusion processes is a key strategy to meet the increasing demands for cell and gene therapy applications. However, perfusion processes are associated with higher medium consumption. Therefore, it is necessary to develop appropriate small‐scale models to reduce development costs. In this work, we present the use of the acoustic wave separation technology in combination with the Ambr 250 high throughput bioreactor system for intensified perfusion process development using stable LV producer cells. The intensified perfusion process developed in the Ambr 250 model, performed at a harvest rate of 3 vessel volumes per day (VVD) and high cell densities, resulted in a 1.4‐fold higher cell‐specific functional virus yield and 2.8‐fold higher volumetric virus yield compared to the control process at a harvest rate of 1 VVD. The findings were verified at bench scale after optimizing the bioreactor set‐up, resulting in a 1.4‐fold higher cell‐specific functional virus yield and 3.1‐fold higher volumetric virus yield.
Comparison of augmented reality with other teaching methods in learning anatomy: A systematic review
Authors: Williams Ally , Sun Zhonghua , Vaccarezza Mauro 
Published: November 2024

AbstractAugmented reality (AR) has been investigated as an engaging educational tool that motivates undergraduate health science students to learn human anatomy. AR technology is developing rapidly, supporting medical education by presenting models of human anatomy as digital objects overlaid in the real world via mobile or head‐mounted display (HMD). The purpose of this systematic review is to provide a post‐pandemic analysis of AR compared with other methods for teaching anatomy and to determine the effects of AR on learning outcomes (LOs). Original research published between January 2020 and April 2024 was obtained from the WOS, Scopus, MEDLINE(Ovid), EMBASE, and PubMed databases, following PRISMA 2020 protocols. Articles included for analysis compared AR with alternative pedagogical methods of teaching undergraduate human anatomy. Studies that described AR as “mixed reality” (MR) were included, while those describing “virtual reality” (VR) were not considered. Risk of bias and limitations in individual studies were assessed using the Quality Assessment with Diverse Studies (QuADS) tool. Data were synthesized using a convergent integrated approach with LOs tabulated for visual analysis. A total of 17 articles were eligible for review: nine studies comparing AR via HMD hardware, and eight comparing mobile AR. The LOs of 12 studies were overwhelmingly reported as non‐significant. Insufficient data precluded an accurate meta‐analysis of LOs, and critical analysis revealed a considerable risk of bias and lack of justified methodology. While AR holds potential for supporting undergraduate students in learning anatomy, definitive outcomes from the current literature are limited by the heterogeneous nature of the studies and inconsistent use of terminology. It is recommended that future research employs professional AR technologies and incorporates the perspectives of university educators to ensure reliable outcomes that can direct the further development of AR technology in medical education.
Psychological Distress in Bladder Cancer Patients: A Systematic Review
Authors: Thomas Kezia Reji , Joshua Catherine , Ibilibor Christine 
Published: November 2024

ABSTRACTObjectiveBladder cancer patients experience high levels of disease and treatment‐related distress, however, factors that can mitigate patient‐reported psychological distress are poorly characterized. Thus, this study serves to summarize the burden of psychological distress among bladder cancer patients and identify clinical, psychological, and socioeconomic factors that are associated with varying levels of psychological distress.MethodsWe performed a systematic review of studies examining psychological distress in bladder cancer patients. We searched PubMed/MEDLINE, Embase, and PsycINFO from October 2000 to February 2024 according to the PRISMA guidelines. Associations between clinical, psychological, socioeconomic factors, and psychological distress were identified in each study and extracted. The protocol for this review is registered in PROSPERO (CRD42024495568).ResultsUsing our search strategy, 759 articles were retrieved and 17 met inclusion criteria, representing 2572 bladder cancer patients. Tumor stage (n = 3), younger age (n = 2), female sex (n = 2) the preoperative setting (n = 2), depression/anxiety (n = 2), and negative psychological response to illness (n = 2) were common factors associated with increased psychological distress. Transitioning from the preoperative to the postoperative period (n = 2), postoperative inpatient rehabilitation (n = 2), feeling well informed (n = 2), and social support (n = 2) were associated with decreased psychological distress.ConclusionWhile clinical factors associated with increased psychological distress are nonmodifiable, clinical, psychological, and socioeconomic factors associated with decreased psychological distress can be improved upon by healthcare providers to mitigate the distress that bladder cancer patients experience.
Synthesis and Fungicidal Activity Evaluation of Novel Triazole Thione/Thioether Derivatives Containing a Pyridylpyrazole Moiety
Authors: Fan Wenqi , Huang Xiaobing , Yu Shujing , Bian Qiang , Wang Baolei 
Published: November 2024

AbstractCompounds containing N‐pyridylpyrazole motif have aroused interest and brought about research hotspots due to their highly‐efficient insecticidal activity. The fungicidal potential of N‐pyridylpyrazole derivatives has gradually been disclosed in recent years. To discover new agrochemicals with poly‐heterocyclic features, a series of novel triazole thione/thioether derivatives containing pyridylpyrazole motif (8–11) was synthesized. The new compounds were identified by melting point, 1H‐NMR, 13C NMR, 19F NMR, HRMS, and elemental analysis. The bioassays showed that most of the pyridylpyrazole‐containing triazole thione Mannich bases possessed favorable in vitro fungicidal activity toward pathogenic fungi, such as Magnaporthe oryzae, Sclerotinia sclerotiorum, Botrytis cinerea and Fusarium verticillioides, and were comparable with those of the contrast compounds A and triadimefon. Some of them exhibited moderate to good in vivo fungicidal activity against S. sclerotiorum at 0.2 mg/mL (e. g. 8f control efficacy: 60.9±3.2 %). The SEM observation displayed that 8f might cause disruption of cell membrane and wall of S. sclerotiorum. Compounds 8a, 8c, 8f–8h, 8p and 9b can serve as promising new fungicidal agents to make further structural optimization. The findings in this article provide useful clue and guidance for the design and development of new poly‐heterocyclic agrochemicals.
SIADH secondary to rhino‐orbito‐cerebral mucormycosis: A case report
Authors: Shrestha Bibek , Shrestha Pradeep , Shrestha Prabin , Bastakoti Sudip , Gupta Prahlad , Magar Shiva Ram Ale 
Published: November 2024

Key Clinical MessageInvasive fungal mucormycosis and its outcome as SIADH and orbital apex syndrome is uncommon. Mucormycosis in paranasal sinuses can even lead to intracranial invasion and its treatment with the use of amphotericin B can cure improve the prognosis of the disease. Early diagnosis and prompt treatment with antifungal, endoscopic surgery, and controlling of diabetes can be beneficial.AbstractMucormycosis is an opportunistic invasive fungal infection which is rare and fatal and can even cause intracranial invasion leading to SIADH. The infrequency with which mucormycosis with SIADH is encountered makes it a formidable diagnostic challenge. 70‐year‐old women uncontrolled diabetes mellitus presented with bilious vomiting and persistent headache with ptosis, proptosis, absence of extraocular movement, pupillary light reflex, and light perception of left eye. Radiographic investigation, KOH mount, and Biopsy showed mucormycosis in sinus with intracranial extension leading to SIADH. Further investigation revealed hyponatremia, decreased plasma osmolality. Then, when diabetes was controlled and hydrocortisone and amphotericin was given along with Endoscopic sinus debridement, SIADH was well controlled. This case illustrates the potential of mucormycosis in paranasal sinuses can even lead to intracranial invasion and its treatment with the use of amphotericin B can cure improve the prognosis of the disease. Prompt diagnosis through clinical history, radiological investigation, and laboratory parameters are important and its treatment is crucial for the better prognosis.
Prognosis and diagnosis prediction of lung adenocarcinoma outcome based on a novel model anchored in circadian clock‐related genes
Authors: Perbal Bernard 
Published: November 2024

AbstractThe transition of JCCS from Springer to Wiley was rich in future prospects and was confirmed by the Journal reorganization launched in 2019. However, in spite of my recently renewed demand, the “B&B” section of the Journal (Bits and Bytes not be confused with Bed and Breakfast, albeit…) did not fit into the Wiley Publishing categories and had to be dropped, to the great disappointment of many authors and our readership. This Editorial will fill the gap by discussing new published aspects on the topic of the connections existing between circadian cellular signaling and cancer prognosis, with the identification of eight genetically significant clock‐related genes in lung cancer.
Sustainable Production of BioAIE Materials From Biomass
Authors: Zhong Weiren , Lin Yuting , Zhao Zhichen , Cai Xu‐Min , Zhang Bo 
Published: November 2024

AbstractThe research of aggregation‐induced emission (AIE) materials has aroused extensive interests during the past 20 years. Until recently, biomass‐inspired AIE (BioAIE) materials have become highly attractive owing to the advantages of natural structural diversity, renewability, biocompatibility, and biodegradability of the biomass. In this concept, we focus on the sustainable production of BioAIE materials from biomass resources (biomacromolecules and small natural products) through extraction, chemical conversion, and physical conversion. Chemical conversion is especially stated, including catalytic conversion, schiff base reaction, “in water” reaction, and other chemical modifications. The luminescence mechanism of AIE behaviors along with their structure–property relationships is emphasized, and the applications are addressed as well. An outlook is provided to highlight the challenges and opportunities associated with the future development trend in this field.
Dryland pea seeding rates can be reduced without yield or economic penalty
Authors: Carr Patrick M. , Fordyce Simon I. , Koeshall Samuel T. , Lamb Peggy F. , Miller Perry R. , Torrion Jessica A. , Vetch Justin M. 
Published: November 2024

AbstractMontana is the leading producer of field peas (Pisum sativum L.) in the United States. A density of 8 to 10 plants ft−2 is recommended when growing field peas in that state, but this recommendation is based on work done elsewhere. Field experiments were conducted in central Montana from 2021 through 2023 and at three additional locations in the final year to determine the yield and the economically optimum plant population (EOPP) when growing field peas for grain. The semi‐leafless, yellow‐cotyledon variety Montech 4152 was planted at five rates (5, 7, 9, 11, and 13 pure live seed [PLS] ft−2) in all 3 years with two additional rates (3 and 15 PLS ft−2) added in the final year. A minimum plant density of 6 to 8 plants ft−2, or planting field pea at 7 to 9 PLS ft−2, produced a grain yield comparable to or greater than amounts produced at other seeding rates. The minimum EOPP across the six experiments ranged from 3 (2.8) to 7 (6.4) plants ft−2, corresponding to a seeding rate of 3 to 7 PLS ft−2. However, more weeds were observed when field pea was planted at 3 PLS ft−2 than at higher rates in one of the experiments. A field pea density of 6 to 8 plants ft−2 is sufficient to optimize grain yield and economic returns in Montana and similar dryland environments.
Analysis of Macromolecular Size Distributions in Concentrated Solutions
Authors: Chaturvedi Sumit K. , Schuck Peter 
Published: November 2024

AbstractThe solution state of macromolecules in concentrated solutions impacts fields ranging from cell biology, to colloid chemistry and engineering of protein pharmaceuticals. Dependent on the interplay between repulsive and weakly attractive forces, proteins may exhibit oligomerization, aggregation, crystallization, liquid‐liquid phase separation, or the formation of multiprotein complexes. The particle size‐distribution is a key characteristic, but difficult to determine when interparticle distances are on the order of their size and macromolecular motion is coupled through hydrodynamic interactions. Here we extend sedimentation velocity analytical ultracentrifugation to measure macromolecular size distributions under these conditions: We apply results from statistical fluid mechanics for the concentration‐dependence of hindered settling and diffusion, embedded in a mean‐field approximation that can resolve coupled sedimentation and diffusion processes of different sized species given experimental sedimentation data. This is combined with a description of transient optical aberrations from lensing in the refractive index gradients associated with sedimentation boundaries (Wiener skewing). We demonstrate this approach in the application to protein solutions with macromolecular volume fractions up to ≈10 %, for example, resolving monomers and dimers of albumin at 140 mg/ml. This enables size‐distribution analysis of proteins at concentrations of therapeutic antibody formations and close to physiological concentration in serum and cells.
Efficient CRISPR/Cas9 Knock‐in Approaches for Manipulation of Endogenous Genes in Human B Lymphoma Cells
Authors: Murray‐Nerger Laura A. , Gewurz Benjamin E. 
Published: November 2024

AbstractPrecise understanding of temporally controlled protein‐protein interactions, localization, and expression is often difficult to achieve using traditional overexpression techniques. Recent advances have made CRISPR‐based knock‐in approaches efficient, which enables rapid derivation of cells with tagged endogenous proteins. However, the high degree of variability in knock‐in efficiency across cell types and gene loci poses challenges, in particular with B lymphocytes, which are refractory to lipid transfection. Here, we present detailed protocols for efficient B lymphoma cell CRISPR/Cas9‐mediated knock‐in. We address knock‐in efficiency in two ways. First, we provide a detailed approach for assessing cutting efficiency to select the most efficient single guide RNA for the gene region of interest. Second, we provide detailed approaches for tagging endogenous proteins with a fluorescent marker or instead for co‐expressing them with an unlinked fluorescent marker. Either approach facilitates downstream selection of single‐cell or bulk populations with the desired knock‐in, particularly when knock‐in efficiency is low. The utility of this approach is demonstrated via examples of engineering tags onto endogenous protein N‐ or C‐termini, together with downstream analyses. We anticipate that this workflow can be applied more broadly to other cell types for efficient knock‐in into endogenous loci. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Choosing an optimal knock‐in target site and single guide RNA (sgRNA) designBasic Protocol 2: Assessment of Cas9 editing efficiency at the desired B cell genomic knock‐in siteBasic Protocol 3: Cloning the sgRNA dual guide constructBasic Protocol 4: Repair template design and cloningBasic Protocol 5: Electroporation and selection of engineered B cellsBasic Protocol 6: Single‐cell cloning of engineered B cells