bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multidisciplinary evaluation of Clostridium butyricum clonality isolated from preterm neonates with necrotizing enterocolitis in South France between 2009 and 2017
Authors: Hosny Michel , Bou Khalil Jacques Yaacoub , Caputo Aurelia , Abdallah Rita Abou , Levasseur Anthony , Colson Philippe , Cassir Nadim , La Scola Bernard 
Published: February 2019

AbstractThe association between Clostridium species identification from stool samples in preterm neonates and the occurrence of necrotizing enterocolitis has been increasingly reported. To confirm the specific impact of Clostridium butyricum in this pathology, selective culture procedure was used for Clostridia isolation. Whole-genome analysis was employed to investigate genomic relationships between isolates. Stool samples from present study, as well as from previously investigated cases, were implicated including 88 from preterm neonates with necrotizing enterocolitis and 71 from matched controls. Quantitative real-time polymerase chain reaction was performed to evaluate the presence of C. butyricum from stools of new cases. Clostridium species prevalence isolated by culture was compared between patients with necrotizing enterocolitis and controls. By combining results of both culture and quantitative polymerase chain reaction methods, C. butyricum was significantly more frequent in stool samples from preterm neonates with necrotizing enterocolitis than in controls. Whole-genome analysis of 81 genomes including 58 neonates’ isolates revealed that cases were clustered depending on geographical origin of isolation. Controls isolates presented genomic relations with that of patients suggesting a mechanism of asymptomatic carriage. Overall, this suggests an epidemiology comparable to that observed in Clostridium difficile colitis in adults.
Metformin inhibits lithocholic acid-induced interleukin 8 upregulation in colorectal cancer cells by suppressing ROS production and NF-kB activity
Authors: Nguyen Thi Thinh , Ung Trong Thuan , Li Shinan , Lian Sen , Xia Yong , Park Sun Young , Do Jung Young 
Published: February 2019

AbstractMetformin, an inexpensive, well-tolerated oral agent that is a commonly used first-line treatment for type 2 diabetes, has become the focus of intense research as a potential anticancer agent. In this study, we describe the inhibitory effect of metformin in interleukin 8 (IL-8) upregulation by lithocholic acid (LCA) in HCT116 colorectal cancer (CRC) cells. Pharmacological inhibition studies indicated that reactive oxygen species (ROS) were involved in LCA-induced IL-8 upregulation through activation of the transcription factor NF-κB. Metformin was demonstrated to block LCA-stimulated ROS production, in turn suppressing NF-κB signaling that was critical for IL-8 upregulation. An NADPH oxidase assay proved that the inhibitory effect of metformin on ROS production was derived from its strong suppression of NADPH oxidase, a key producer of ROS in cells. Compared with conditioned media (CM) derived from HCT116 cells treated with LCA, CM derived from HCT116 cells pretreated with metformin and then treated with LCA lost all stimulatory effect on endothelial cell proliferation and tubelike formation. In conclusion, metformin inhibited NADPH oxidase, which in turn suppressed ROS production and NF-κB activation to prevent IL-8 upregulation stimulated by LCA; this prevention thus obstructed endothelial cell proliferation and tubelike formation.
Heart beat but not respiration is the main driving force of the systemic venous return in the Fontan circulation
Authors: Gabbert Dominik Daniel , Hart Christopher , Jerosch-Herold Michael , Wegner Philip , Salehi Ravesh Mona , Voges Inga , Kristo Ines , Bulushi Abdullah A. L. , Scheewe Jens , Kheradvar Arash , Kramer Hans-Heiner , Rickers Carsten 
Published: February 2019

AbstractThe Fontan procedure provides relief from cyanosis in patients with univentricular hearts. A major clinical unmet need is to understand whether the venous flow patterns of the Fontan circulation lead to the development of congestive hepatopathy and other life-threatening complications. Currently, there is no consensus on whether heart beat or respiration is the main driving force of venous return and which one affects the periodic flow changes for the most (i. e., pulsatility). The present study, for the first time, quantified respiratory and cardiac components of the venous flow in the inferior vena cava (IVC) of 14 Fontan patients and 11 normal controls using a novel approach (“physio-matrix”). We found that in contrast to the normal controls, respiration in Fontan patients had a significant effect on venous flow pulsatility, and the ratio of respiration-dependent to the cardiac-dependent pulsatility was positively associated with the retrograde flow. Nevertheless, the main driving force of net IVC flow was the heart beat and not respiration. The separate analysis of the effects of respiration and heart beat provides new insights into the abnormal venous return patterns that may be responsible for adverse effects on liver and bowel of the patients with Fontan circulation.
Intracellular toxic advanced glycation end-products in cardiomyocytes may cause cardiovascular disease
Authors: Takata Takanobu , Sakasai-Sakai Akiko , Ueda Tadashi , Takeuchi Masayoshi 
Published: February 2019

AbstractCardiovascular disease (CVD) is a lifestyle-related disease (LSRD) and one of the largest public health issues. Risk factors for CVD correlate with an excessive intake of glucose and/or fructose, which has been shown to induce the production of advanced glycation end-products (AGEs). We previously identified AGEs derived from glyceraldehyde and named them toxic AGEs (TAGE) due to their cytotoxicities and relationship with LSRD. We also reported that extracellular TAGE in the vascular system may promote CVD and that serum TAGE levels are associated with risk factors for CVD. The mechanisms responsible for the onset and/or progression of CVD by extracellular TAGE or the above risk factors involve vascular disorders. In the present study, we revealed that rat primary cultured cardiomyocytes generated intracellular TAGE, which decreased beating rates and induced cell death. LC3-II/LC3-I, a factor of autophagy, also decreased. Although intracellular TAGE may be targets of degradation as cytotoxic proteins via autophagy, they may inhibit autophagy. Furthermore, the mechanisms by which intracellular TAGE decrease beating rates and induce cell death may involve the suppression of autophagy. The present results suggest that intracellular TAGE are generated in cardiomyocytes and directly damage them, resulting in CVD.
Neurotrophin receptor Ntrk2b function in the maintenance of dopamine and serotonin neurons in zebrafish
Authors: Sahu Madhusmita Priyadarshini , Pazos-Boubeta Yago , Pajanoja Ceren , Rozov Stanislav , Panula Pertti , Castrén Eero 
Published: February 2019

AbstractNeurotrophins and their receptors have highly conserved evolutionary lineage in vertebrates including zebrafish. The NTRK2 receptor has two isoforms in zebrafish, Ntrk2a and Ntrk2b. The spatio-temporal expression pattern of bdnf and ntrk2b in the zebrafish brain was studied using in situ hybridization. The robust and corresponding expression pattern of ntrk2b to bdnf suggests that ntrk2b is the key receptor for bdnf in the zebrafish brain, unlike its duplicate isoform ntrk2a. To study ntrk2b function, two different genetic strategies, the TILLING mutant and morpholino oligonucleotides (MO), were used. Specific subsets of the dopaminergic and serotonergic neuronal populations were affected in the mutants and morphants. The mutant showed anxiety- like behavior both in larval and adult stages. Our results consistently indicate that BDNF/NTRK2 signaling has a significant role in the development and maintenance of aminergic neuronal populations. Therefore, the ntrk2b-deficient zebrafish is well suited to study mechanisms relevant for psychiatric disorders attributed to a dysfunctional monoaminergic system.
Structural and molecular correlates of cognitive aging in the rat
Authors: Mota Cristina , Taipa Ricardo , das Neves Sofia Pereira , Monteiro-Martins Sara , Monteiro Susana , Palha Joana Almeida , Sousa Nuno , Sousa João Carlos , Cerqueira João José 
Published: February 2019

AbstractAging is associated with cognitive decline. Herein, we studied a large cohort of old age and young adult male rats and confirmed that, as a group, old rats display poorer spatial learning and behavioral flexibility than younger adults. Surprisingly, when animals were clustered as good and bad performers, our data revealed that while in younger animals better cognitive performance was associated with longer dendritic trees and increased levels of synaptic markers in the hippocampus and prefrontal cortex, the opposite was found in the older group, in which better performance was associated with shorter dendrites and lower levels of synaptic markers. Additionally, in old, but not young individuals, worse performance correlated with increased levels of BDNF and the autophagy substrate p62, but decreased levels of the autophagy complex protein LC3. In summary, while for younger individuals “bigger is better”, “smaller is better” is a more appropriate aphorism for older subjects.
STK33/ERK2 signal pathway contribute the tumorigenesis of colorectal cancer HCT15 cells
Authors: Zhang Shengjun , Wu Haoyu , Wang Kaiyu , Liu Minli 
Published: February 2019

Abstract
Serine/threonine kinase 33 (STK33) is a serine/threonine kinase and participates in many apoptotic process. Herein, we found that the extracellular signal-regulated kinase 2 (ERK2) was a substrate of STK33. STK33 phosphorylated ERK2 and increased the activity of ERK2 and promote the tumorigenesis of colorectal cancer HCT15 cells. Clinical simple showed that STK33 was highly expression in colorectal cells and tissues. Ex vivo and in vivo studies demonstrated that STK33 accelerate tumorigenic properties in NCM460 cells and athymic nude rats. In vitro kinase assay results indicated that STK33 can phosphorylate ERK2. Ex vivo studies further showed that STK33 can bind with ERK2 and take part in the regulation of ERKs signaling pathway. In short, our results showed that STK33 is a novel upstream kinase of ERK2. It may provide a better prospect for STK33 based prevention and treatment for colorectal cancer patients.
MKP2 suppresses TGF-β1-induced epithelial-to-mesenchymal transition through JNK inhibition
Authors: Loeffler Ivonne 
Published: February 2019

Abstract
Interstitial fibrosis is a typical feature of end-stage renal diseases, regardless of the initial cause of kidney injury. Epithelial-to-mesenchymal transition (EMT) is a mechanism that is thought to play a role in generating the interstitial matrix-producing myofibroblasts and is prominently induced by the transforming growth factor-β 1 (TGF-β1). TGF-β1 signals through a variety of Smad and non-Smad signaling pathways, including the mitogen-activated protein kinase (MAPK) pathways. In a study published in a recent issue of Clinical Science (Clin. Sci. (2018) 132(21),2339–2355), Li et al. investigated the potential role of the Mitogen-activated protein kinase phosphatase 2 (MKP2), also known as Dusp4, in the control of EMT and renal fibrosis. Based on results obtained with an animal model of kidney fibrosis and a proximal tubular epithelial cell line system, the authors put forward a role for MKP2 as a negative feedback regulator of TGF-β1-induced EMT and fibrosis in the kidney. Intriguingly, MKP2 is found to down-regulate activity of c-Jun, but not that of other MAPKs, extracellular signal-regulated kinases or p38, implying a role for c-Jun N-terminal kinase-dependent signaling in renal fibrosis. In this commentary, I discuss the findings of Li and co-workers in the context of the recent literature placing a focus on potential clinical/therapeutic implications.
The Belgian repository of fundamental atomic data and stellar spectra (BRASS)
Authors: Laverick M. , Lobel A. , Royer P. , Merle T. , Martayan C. , van Hoof P. A. M. , Van der Swaelmen M. , David M. , Hensberge H. , Thienpont E. 
Published: February 2019

Context. Fundamental atomic transition parameters, such as oscillator strengths and rest wavelengths, play a key role in modelling and understanding the chemical composition of stars in the universe. Despite the significant work under way to produce these parameters for many astrophysically important ions, uncertainties in these parameters remain large and can limit the accuracy of chemical abundance determinations.Aims. The Belgian repository of fundamental atomic data and stellar spectra (BRASS) aims to provide a large systematic and homogeneous quality assessment of the atomic data available for quantitative spectroscopy. BRASS shall compare synthetic spectra against extremely high-quality observed spectra, at a resolution of ∼85 000 and signal-noise ratios of ∼1000, for approximately 20 bright BAFGK spectral-type stars, in order to critically evaluate the atomic data available for over a thousand potentially useful spectral lines.Methods. A large-scale homogeneous selection of atomic lines is performed by synthesising theoretical spectra of literature atomic lines for FGK-type stars including the Sun, resulting in a selection of 1091 theoretically deep and unblended lines in the wavelength range 4200–6800 Å, which may be suitable for quality assessment. Astrophysical log(g f) values are determined for the 1091 transitions using two commonly employed methods. The agreement of these log(g f) values are used to select well-behaved lines for quality assessment.Results. We found 845 atomic lines to be suitable for quality assessment, of which 408 were found to be robust against systematic differences between analysis methods. Around 53% of the quality-assessed lines were found to have at least one literature log(g f) value in agreement with our derived values, though the remaining values can disagree by as much as 0.5 dex. Only ∼38% of Fe Ilines were found to have sufficiently accurate log(g f) values, increasing to ∼70–75% for the remaining Fe-group lines.
Herschel water maps towards the vicinity of the black hole Sgr A*
Authors: Armijos-Abendaño J. , Martín-Pintado J. , Requena-Torres M. A. , González-Alfonso E. , Güsten R. , Weiß A. , Harris A. I. , Israel F. P. , Kramer C. , Stutzki J. , van der Werf P. 
Published: February 2019

Aims. We study the spatial distribution and kinematics of water emission in a ~8 × 8 pc2 region of the Galactic center (GC) that covers the main molecular features around the supermassive black hole Sagittarius A* (Sgr A*). We also analyze the water excitation to derive the physical conditions and water abundances in the circumnuclear disk (CND) and the “quiescent clouds”.
Methods. We presented the integrated line intensity maps of the ortho 110 − 101, and para 202 − 111 and 111 − 000 water transitions observed using the On the Fly mapping mode with the Heterodyne Instrument for the Far Infrared (HIFI) on board Herschel. To study the water excitation, we used HIFI observations of the ground state ortho and para H218O transitions toward three selected positions in the vicinity of Sgr A*. In our study, we also used dust continuum measurements of the CND, obtained with the Spectral and Photometric Imaging REceiver (SPIRE) instrument. Using a non-local thermodynamical equilibrium (LTE) radiative transfer code, the water line profiles and dust continuum were modeled, deriving H2O abundances (XH2O), turbulent velocities (V t), and dust temperatures (Td). We also used a rotating ring model to reproduce the CND kinematics represented by the position velocity (PV) diagram derived from para 202 − 111 H2O lines.
Results. In our H2O maps we identify the emission associated with known features around Sgr A*: CND, the Western Streamer, and the 20 and 50 km s−1 clouds. The ground-state ortho water maps show absorption structures in the velocity range of [−220,10] km s−1 associated with foreground sources. The PV diagram reveals that the 202 − 111 H2O emission traces the CND also observed in other high-dipole molecules such as SiO, HCN, and CN. Using the non-LTE code, we derive high XH2O of ~(0.1–1.3) × 10−5, V t of 14–23 km s−1 , and Td of 15–45 K for the CND, and the lower XH2O of 4 × 10−8 and V t of 9 km s−1 for the 20 km s−1 cloud. Collisional excitation and dust effects are responsible for the water excitation in the southwest lobe of the CND and the 20 km s−1 cloud, whereas only collisions can account for the water excitation in the northeast lobe of the CND. We propose that the water vapor in the CND is produced by grain sputtering by shocks of 10–20 km s−1, with some contribution of high temperature and cosmic-ray chemistries plus a photon-dominated region chemistry, whereas the low XH2O derived for the 20 km s−1 cloud could be partially a consequence of the water freeze-out on grains.
Risiko Impfen?
Authors: Kowalzik Frank , Zepp Fred 
Published: February 2019

AbstractSkepticism about vaccination is not new, but the perception of vaccine-related risks has changed in recent decades. In addition to aspects such as knowledge about vaccines, trust in medical care structures and political institutions, socio-economic, cultural and religious views also play an important role. These factors differ significantly worldwide and regionally. In almost all surveys, confidence in vaccines and/or the healthcare system is identified as essential for a positive vaccination decision. Confidence in vaccines correlates with the assessment of the specific individual disease risk and the potential side effects of the vaccines. Subjective perception of risk seldom corresponds to objectively measurable facts. Rather, it is distorted by individual perception in both directions (underestimation and overestimation). Transparent, science-based communication is helpful in promoting and maintaining trust in healthcare. Pediatricians and family physicians are the most important confidants for parents on questions about health care and vaccination recommendations.
Adhärenz von Hebammensprechstunden – eine quantitative Dokumentenanalyse
Authors: Siegle Anja , Sayn-Wittgenstein Friederike , Roes Martina 
Published: February 2019

Zusammenfassung
Hintergrund Geburtshilfliche Interventionen nehmen weltweit zu, auch in Deutschland. Um deren Zahl zu reduzieren entstehen verschiedene Ansätze zur Förderung der normalen Geburt, so auch der deutsche „Expertinnenstandard Förderung der physiologischen Geburt“. Unter anderem wird darin eine Hebammensprechstunde gefordert. Inwieweit Hebammensprechstunden in Einrichtungen, die den Expertinnenstandard eingeführt haben, implementiert sind und ob sie in ihrer Ausgestaltung den Empfehlungen, so wie sie von den Entwicklerinnen intendiert waren (Adhärenz), folgen, ist bisher nicht bekannt. Ziel dieser Studie ist es, Analysemerkmale für die Hebammensprechstunde zu entwickeln, die Zielgruppe und Inhalte zu identifizieren und die Adhärenz zu untersuchen.
Methode Eine Delphi-Befragung zur Entwicklung von Analysemerkmalen mit 3 Entwicklerinnen und eine quantitative Dokumentenanalyse der Dokumente der Hebammensprechstunden zweier Krankenhäuser wurde durchgeführt (Krankenhaus 1: n=92; Krankenhaus 2: n=62).
Ergebnisse Sechs Merkmale (Dokumentation von „Sorgen und Ängste“, „Absprachen“, „Beratungsthemen“, „Themen, die besprochen wurden“, „Nachvollziehbarkeit der Möglichkeiten der Geburtsplanung“ und „Optionen der Geburtsplanung) wurden aus dem Expertinnenstandard identifiziert. Vor allem Erstgebärende (77,2%) nehmen die Hebammensprechstunde in Anspruch. Es zeigt sich ein breites Spektrum an Themen, die im Rahmen der Hebammensprechstunde angesprochen werden. Die Dokumente von Krankenhaus 2 belegen eine hohe Adhärenz, während die Dokumente von Krankenhaus 1 eine mittlere Adhärenz zeigen.
Schlussfolgerungen Die Untersuchung der Adhärenz ermöglicht es Krankenhäusern, die Hebammensprechstunde auf ihre Umsetzung hin einzuschätzen. Auf Grundlage der hier vorgestellten Analyse können Anpassungen, z. B. an der Dokumentenstruktur oder den Beratungsinhalten vorgenommen werden. Im Kontext der Hebammensprechstunde besteht weiterer Forschungsbedarf zu den Implementierungsprozessen, um vorhandene Unterschiede in der Adhärenz besser zu erklären.
Congenital Hypopituitarism: Various Genes, Various Phenotypes
Authors: Xatzipsalti Maria , Voutetakis Antonis , Stamoyannou Lela , Chrousos George , Kanaka-Gantenbein Christina 
Published: February 2019

AbstractThe ontogenesis and development of the pituitary gland is a highly complex process that depends on a cascade of transcription factors and signaling molecules. Spontaneous mutations and transgenic murine models have demonstrated a role for many of these factors, including HESX1, PROP1, PIT1, LHX3, LHX4, SOX2, SOX3, OTX2, PAX6, FGFR1, SHH, GLI2, and FGF8 in the etiology of congenital hypopituitarism. Genetic mutations in any of these factors can lead to congenital hypopituitarism, which is characterized by the deficiency in one or more pituitary hormones. The phenotype can be highly variable, consisting of isolated hypopituitarism or more complex disorders. The same phenotype can be attributed to different gene mutations; while a given gene mutation can induce different phenotypes. This review highlights the genetic variations that lead to congenital hypopituitarism and their associated defects. The overall incidence of mutations in known transcription factors in patients with hypopituitarism is low; therefore many gene mutations or even gene- epigenetic interactions have to be unraveled in the future to explain the vast majority of still unclear cases of congenital hypopituitarism.
Isopathic Use of Auto-Sarcode of DNA as Anti-Miasmatic Homeopathic Medicine and Modulator of Gene Expression?
Authors: Teixeira Marcus 
Published: February 2019

Introduction In addition to the four pillars of homeopathy, vitalism and the miasmatic theory are often used to explain the health–disease process. According to Hahnemann's concepts, homeopathic miasms are the main obstacle to the cure of chronic diseases, with psora being the fundamental cause of all forms of diseases. According to modern genetics, the disease-promoting epigenetic alterations are the fundamental cause of the manifestation of chronic diseases.
Objective This article develops a philosophical–scientific correlation between chronic miasms and disease-promoting epigenetic modifications, aiming to justify the isopathic use of auto-sarcode of an individual's DNA as homeopathic medicine.
Results Based on the study of homeopathic doctrine and epigenetics, a conceptual and functional correlation is observed between homeopathic chronic miasms and disease-promoting epigenetic modifications. Additionally, several experimental studies suggest that homeopathy's mechanism of action may be by modulating gene expression.
Conclusions By the philosophical–scientific correlations described, it is inferred that disease-promoting epigenetic alterations are the biological representation of the chronic miasms, suggesting the isopathic use of auto-sarcode of DNA as homeopathic therapeutic modulator of gene expression for the management of chronic diseases.