bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative analysis of outer membrane vesicles proteomics and whole-cell transcriptome analysis of eravacycline induced Acinetobacter baumannii strains
Authors: Kesavan Dinesh Kumar , Vasudevan Aparna , Wu Liang , Chen Jianguo , Su Zhaoliang , Wang Shengjun , Xu Huaxi 
Published: October 2019

Abstract
Background Acinetobacter baumannii is a multidrug resistant (MDR) hazardous bacterium with very high antimicrobial resistance profiles. Outer membrane vesicles (OMVs) help directly and/or indirectly towards antibiotic resistance in these organisms. The present study aims to look on the proteomic profile of OMV as well as on the bacterial transcriptome upon exposure and induction with eravacycline, a new synthetic fluorocycline. RNA sequencing analysis of whole cell and LC–MS/MS proteomic profiling of OMV proteome abundance were done to identify the differential expression among the eravacycline-induced A. baumannii ATCC 19606 and A. baumannii clinical strain JU0126.Results The Differential expressed genes from the RNA sequencing and were analysed using R package and bioinformatics software and tools. Genes encoding drug efflux and membrane transport were upregulated among the DEGs from both ATCC 19606 and JU0126 strains. As evident with the induction of eravacycline resistance, ribosomal proteins were upregulated in both the strains in the transcriptome profiles and also resistance pumps, such as MFS, RND, MATE and ABC transporters. High expression of stress and survival proteins were predominant in the OMVs proteome with ribosomal proteins, chaperons, OMPs OmpA, Omp38 upregulated in ATCC 19606 strain and ribosomal proteins, toluene tolerance protein, siderophore receptor and peptidases in the JU0126 strain. The induction of resistance to eravacycline was supported by the presence of upregulation of ribosomal proteins, resistance conferring factors and stress proteins in both the strains of A. baumannii ATCC 19606 and JU0126, with the whole-cell gene transcriptome towards both resistance and stress genes while the OMVs proteome enriched more with survival proteins.Conclusion The induction of resistance to eravacycline in the strains were evident with the increased expression of ribosomal and transcription related genes/proteins. Apart from this resistance conferring efflux pumps, outer membrane proteins and stress related proteins were also an essential part of the upregulated DEGs. However, the expression profiles of OMVs proteome in the study was independent with respect to the whole cell RNA expression profiles with low to no correlation. This indicates the possible role of OMVs to be more of back-up additional protection to the existing bacterial cell defence during the antibacterial stress.
Protection of L1 nerve roots in vertebral osteotomy for severe rigid thoracolumbar spine deformity
Authors: Liao Hang , Miao Houguang , Xie Peng , Wang Yueyue , Li Ningdao , Zheng Guizhou , Li Xuedong , Du Shixin 
Published: October 2019

Abstract
Background: This is a retrospective study of the use of parallel endplate osteotomy (PEO) for correction of severe rigid thoracolumbar spine deformity. Methods : From July 2016 to June 2017, 10 patients with severe rigid thoracolumbar spine deformity underwent PEO on T12 or L1 vertebrae were studied. Results : Following PEO performed at T12 or L1, the kyphosis and scoliosis correction rates reached averages of 77.4 ± 8.5% and 76.6 ± 6.8%, intraoperative bleeding 1990 ± 1010 ml, operation time 7.12 ± 3.88 h. One year after surgery, the SF-36 scores of physical function, role-physical, bodily pain, general health, vitality, social function, role-emotional and mental health from 60 ± 30, 47 ± 33, 44 ± 30, 32 ± 18, 50 ±30, 46 ± 29, 26 ± 40 and 52 ± 20 to 81 ± 16, 69 ± 19, 73 ± 11, 66 ± 21, 74 ± 16, 74 ± 24, 63 ± 37 and 76 ± 12, respectively (P < 0.01). Three patients had symptoms of L1 nerve root injury, as reflected by knee extension and hip flexion of lower limb weakness and inner thigh numbness, which further confirmed by electromyography. Conclusions : The parallel endplate osteotomy is simple, results in less bleeding, spinal cord and nerve root under direct vision and can effectively and safely correct severe rigid thoracolumbarspine deformity with better clinical results. However, it is important to identify, separate and protect L1 nerve roots during surgery in cases where patients have symptoms of back pain, muscle weakness and leg numbness on the convex side after surgery. Key words: Thoracolumbar deformity, Nerve roots injury, L1 nerve roots, Parallel endplate osteotomy
Significant association between serum resistin and hypersensitive troponin I levels in patients with a first ST-segment elevation myocardial infarction
Authors: Zhu Yong , Hu Chengping , Du Yu , Liu Yan , Liu Jinxing , Zhang Jianwei , Cheng Guojie , Han Hongya , Zhao Qi , Tong Zichuan , Zhao Yingxin 
Published: October 2019

Abstract
Background Resistin, a proinflammatory adipocytokine secreted predominately by macrophages in humans, plays an important role in the pathogenesis and development of atherosclerosis. The present research mainly investigated the association between serum resistin level and peak hypersensitive cardiac troponin I (hs-cTnI) in patients with ST-segment elevation myocardial infarction (STEMI).Methods We consecutively enrolled 92 patients with a first STEMI in this cross-sectional and observational study. Resistin concentrations upon admission and 24 h and 72 h after primary percutaneous coronary intervention (PCI) were all measured. The change in resistin (δ Resistin) was defined as (serum resistin concentration at admission)-(serum resistin concentration 24 h after intervention).Results Serum resistin concentration decreased rapidly after primary PCI. Resistin at admission correlated positively with tumour necrosis factor-α (r = 0.522, p<0.001) and macrophage migration inhibitory factor (r = 0.471, p<0.001). Additionally, resistin at admission correlated negatively with the reactive oxygen species scavengers superoxide dismutase (r = -0.261, p = 0.012) and glutathione peroxidase (r = -0.235, p = 0.024). Most importantly, serum resistin concentrations upon admission (r = 0.381, p<0.001) and 24 h (r = 0.372, p<0.001) and 72 h (r = 0.347, p = 0.001) after primary PCI all correlated with peak hs-cTnI, while δ Resistin was not associated with peak hs-cTnI. After multiple linear regression analysis, serum resistin (beta = 13.593, 95% CI 5.951 to 21.235, p < 0.001) at admission and 24 h (beta = 13.972, 95% CI 5.662 to 22.282, p = 0.001) and 72 h (beta = 14.455, 95% CI 5.178 to 23.733, p = 0.003) after intervention remained associated with peak hs-cTnI.Conclusions In our present research, serum resistin concentrations at different time points all correlated positively with peak hs-cTnI, which may suggest that serum resistin concentrations during the acute phase of STEMI are useful for forecasting myocardial infarction size and prognosis in patients after primary PCI. Additionally, our research also indicated that resistin may regulate myocardial IRI partly by promoting the inflammatory process and oxidative stress.
Using a Hepatitis B surveillance system evaluation in Fujian, Hainan, and Gansu Provinces to improve data quality and assess program effectiveness, China, 2015
Authors: Zheng Hui , Millman Alexander J. , Rainey Jeanette J. , Wang Fuzhen , Zhang Rui , Chen Hong , Yin Zundong , Wang Huaqing , zhang Guomin 
Published: October 2019

Abstract
Background: Monitoring hepatitis B surveillance data is important for evaluating progress towards global hepatitis B elimination goals. Accurate classification of acute and chronic hepatitis infections is essential for assessing program effectiveness. Methods: We evaluated hepatitis B case-reporting at six hospitals in Fujian, Hainan and Gansu provinces in 2015 to assess the accuracy of case classification. We linked National Notifiable Disease Reporting System (NNDRS) HBV case-reports with hospital information systems and extracted information on age, gender, admission ward and viral hepatitis diagnosis from medical records. To assess accuracy, we compared NNDRS reported case-classifications with the national HBV case definitions. Multivariable logistic regression was used to identify factors associated with misclassification. Results: Of the 1,420 HBV cases reported to NNDRS, 23 (6.5%) of the 352 acute reports and 648 (60.7%) of the 1,068 chronic reports were correctly classified. Of the remaining, 318 (22.4%) were misclassified and 431 (30.4%) could not be classified due to the lack of supporting information. Based on the multivariable analysis, HBV cases reported from Hainan (aOR=1.8; 95% CI: 1.3-2.4) and Gansu (aOR=12.7; 95% CI: 7.7-20.1) along with reports from grade 2 hospitals (aOR=1.6; 95% CI:1.2-2.2) and those from non-HBV related departments (aOR=5.3; 95% CI: 4.1-7.0) were independently associated with being ‘misclassified’ in NNDRS. Conclusions: We identified discrepancies in the accuracy of HBV case-reporting in the project hospitals. Onsite training on the use of anti-HBc IgM testing as well as on HBV case definitions and reporting procedures are needed to accurately assess program effectiveness and ensure case-patients are referred to appropriate treatment and care. Routine surveillance evaluations such as this can be useful for improving data quality and monitoring program effectiveness.
Iron Sulphur Cluster [Fe4S4(SPh)4]2– Catalyzed Electrochemical Reduction of CO2 on Carbon Electrodes in [Bu4N][BF4]-DMF Mixture
Authors: Alenezi Khalaf M. 
Published: October 2019

Background:An efficient, selective and durable electrocatalytic carbon dioxide (CO2) reduction system is a prerequisite to tackle energy and pollution-related issues. In this context, both organic and inorganic materials have gained a significant interest worldwide.Objective:In the present work, the electrocatalytic reduction activity of an iron-sulphur (Fe-S) cluster, [Fe4S4(SPh)4]2– for CO2 → carbon monoxide (CO) conversion has been investigated. The effect of catalyst concentration on the yield of CO and H2 was determined. Besides, the influence of reaction conditions (presence or absence of a Brønsted acid, electrolysis time etc.) on faradaic yield and product selectivity was also investigated.Methods:Cyclic voltammetry (CV) was carried out on vitreous carbon electrode in 0.1 M [Bu4N] [BF4]-DMF electrolyte. At the end of electrolysis, products were collected by tight-gas syringe and analyzed by gas chromatography (GC) system coupled with a thermal conductivity detector.Results:The Fe-S cluster was found to efficiently catalyse the process on carbon electrode in 0.1 M [Bu4N][BF4]-DMF electrolyte. Moreover, the presence of cluster shifted the reduction potential by ~ 200 mV towards the positive. GC analysis confirmed the formation of CO with a current efficiency of ca. 70%. On the other hand, 12% H2 was observed at the end of electrocatalysis.Conclusion:In summary, Fe-S cluster was used for the electrocatalytic reduction of CO2 in 0.1 M [Bu4N][BF4]-DMF electrolyte. The use of cluster (catalyst) was found to be important for CO2 reduction as no CO was detected in the absence of the catalyst. This study highlights the potential application of Fe-S cluster for CO2 reduction.
KOMPETENSI MINIMAL PROFESI SEKRETARIS DIREKSI DI SEPULUH PERUSAHAAN INDONESIA
Authors: Selfiana Selfiana 
Published: October 2019

Abstrak: Kompetensi Minimal Profesi Sekretaris Direksi di Sepuluh Perusahaan Indonesia. Tujuan penelitian ini adalah untuk melakukan pemetaan terhadap kompetensi minimal yang harus dimiliki oleh seorang sekretaris direksi agar memiiki kemampuan yang tinggi guna penyelesaian tugas dan tanggungjawabnya dengan baik. Diharapkan juga agar penelitian ini dapat menjadi rujukan bagi calon sekretaris direksi dalam mempersiapkan dengan tepat dalam membangun kompetensi yang sesuai dengan kebutuhan profesi tersebut. Penelitian ini menggunakan pendekatan deskriptif kualitatif. Hasil penelitian menunjukkan bahwa kompetensi utama yang harus dimiliki sekretaris direksi adalah pribadi yang dapat dipercaya, memiliki perhatian pada pekerjaannya, memiliki pengetahuan berbahasa asing, memiliki keahlian menggunakan bahasa Indonesia dan bahasa asing, memiliki ketrampilan berkomunikasi serta memiliki pengalaman kerja di bidang perkantoran dan tata usaha kantor. Kata kunci: Kompetensi, Sekretaris. Abstract: Minimum Competence Secretary of Director in Ten Indonesian Companies. The purpose of this study is to map the minimum competencies that must be owned by a director's secretary so that they have a high ability to properly complete their duties and responsibilities. It is also hoped that this research can be a reference for prospective secretaries of directors in preparing properly in developing competencies that are in accordance with the needs of the profession. This research use desciptive qualitative approach. The results showed that the main competencies that the directors' secretaries must possess are individuals who can be trusted, have attention to their work, have foreign language knowledge, have expertise in using Indonesian and foreign languages, have communication skills and have work experience in office and office administration. Keyword: Competence, Secretary.
Gambaran Umum Kasus Frambusia setelah Pengobatan Massal dengan Azitromisin di Kota Jayapura
Authors: Arisanti Yuli , Tanjung Ratna , Cahyani Vatim Dwi 
Published: October 2019

Yaws is still unfinished health problem in Jayapura City, there is still have enclave yaw’s disease. This study aimed to know clinical and result of Rapid Test Diagnostic of yaws after mass therapy used azitromisin and to know about sanitation according to yaws. These was descriptive and cross sectional study design. Method of this study are interview, clinical examination, RDT and lesion sampel using Darkfield microscope and microscope. From 229 respondences after mass therapy of azitromisin in Jayapura City consist of 113 boys and 116 girls. The youngest about 3 years old and the oldest about 15 years old. Most of them have already have good personal hygiene from the highest presentance of taking bath, bathing used soap and changing clothes after bathing. Prevalence of yaws are tend to decreased, we found only 5 RDT (+).
Frambusia masih menjadi masalah kesehatan yang belum terselesaikan, masih terdapat daerah kantong frambusia di Kota Jayapura. Penelitian ini bertujuan untuk mendapatkan gambaran secara klinis maupun pemeriksaan RDT frambusia setelah pengobatan massal dan mengetahui data sanitasi terkait frambusia ini. Jenis penelitian yang digunakan yaitu observasional dengan desain potong lintang (cross sectional). Metode yang digunakan terdiri dari wawancara dengan kuesioner, pemeriksaan klinis, pemeriksaan RDT dan pemeriksaan sampel berupa apusan lesi dengan menggunakan mikroskop lapangan gelap dan mikroskop cahaya biasa (pewarnaan gram). Hasil yang didapat berupa data dari 229 responden yang telah mendapatkan pengobatan Azitromisin di Kota Jayapura yang terdiri dari responden laki – laki berjumlah 113 orang dan responden perempuan berjumlah 116 orang. Umur termuda ditemukan berumur 3 tahun dan umur tertinggi 15 tahun. Sebagian besar responden sudah memiliki personal hygiene yang cukup baik dilihat dari tingginya persentase frekuensi mandi, pelaksanaan mandi memakai sabun dan mengganti baju setelah mandi. Angka kasus frambusia di Kota Jayapura cenderung turun dimana ditemukan hanya 5 responden dengan RDT (+).
Pemanfaatan Bengkoang (Pachyrrhizus erosus) dan Tauge (Vigna radiate) sebagai Media Alternatif untuk Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus
Authors: Rizki Zuriani , Syahnitya Hastuty 
Published: October 2019

Penelitian ini berjudul “Media Alami Bengkoang (Pachyrrhizus erosus) dan Tauge (Vigna radiate) sebagai Media Alternatif untuk Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus”. Penelitian ini bertujuan mencari media alami yang berpotensi untuk pertumbuhan mikroorganisme dengan harga yang murah. Hasil penelitian menunjukkan media alami bengkoang (Pachyrrhizus erosus) dan media alami tauge (Vigna radiate) dapat digunakan sebagai media alternatif untuk pertumbuhan bakteri Escherichia coli dan staphylococcus aureus. Berdasarkan hasil penelitian maka dapat disarankan sebaiknya dilakukan penelitian lanjutan tentang media-media alami yang merupakan sumber karbohidrat dan protein sebagai media alternatif pertumbuhan bagi bakteri. Media bengkoang dan media tauge juga dapat di aplikasikan sebagai media alternatif dalam penelitian laboratorium, terutama bidang mikrobiologi.
Significados em puérperas da internaçao na Unidade de terapia intensiva.
Authors: Osorio Contreras Laura Vanessa , Castiblanco López Nubia 
Published: October 2019

Objetivo: Describir el significado desde lo cultural que atribuyen puérperas con Morbilidad Materna Extrema a su hospitalización en la Unidad de Cuidados Intensivos. Materiales y Métodos: Estudio cualitativo con abordaje etnográfico, utilizando como referente teórico a Madeleine Leininger. Las entrevistas a profundidad y el análisis cualitativo se realizaron teniendo en cuenta el método de James Spradley. Se efectuaron 21 entrevistas a profundidad a 9 puérperas con Morbilidad Materna Extrema que estuvieron hospitalizadas en la Unidad de Cuidados Intensivos de una clínica de la ciudad de Sincelejo-Sucre - Colombia entre marzo y diciembre de 2017. Resultados: Los significados que atribuyen puérperas con Morbilidad Materna Extrema sobre su hospitalización en la Unidad de Cuidados Intensivos incluyeron 8 dominios: conocimiento de los signos de alarma, temor a morir, muy difícil para mi compañero, aplazar la lactancia materna, de regreso a la clínica, mi familia me cuida, la enfermera me cuida y aprender de esta experiencia. Conclusiones: Los significados de puérperas con Morbilidad Materna Extrema están colmados de experiencias, creencias culturales y sentimientos, donde el cuidado compasivo y congruente de enfermería les ayuda a sobrevivir y la hospitalización en la UCI se constituye en una enseñanza para la vida.
Resumo
Objetivo: Descrever o significado desde o olhar cultural que atribuem puérperas com morbidade materna extrema a sua internação na Unidade de Terapia Intensiva (UTI). Materiais e Métodos: Estudo qualitativo com abordagem etnográfica, utilizou-se como referente teórico a Madeleine Leininger. As entrevistas a profundidade e a análise qualitativa realizou-se considerando o método de James Spradley. Obtiveramse 21 entrevistas a profundidade em 9 mulheres puérperas com morbidade materna extrema e estiveram internadas numa UTI de uma clínica da cidade de Sincelejo, Sucre, Colômbia entre março e dezembro de 2017. Resultados: Os significados que atribuíram as puérperas com morbidade materna extrema respeito à sua internação na UTI incluíram oito domínios: conhecimento dos sinais de alarma, temor da morte, muito difícil para o meu parceiro, adiamento da amamentação, retorno à clinica, minha família cuida de mim, a enfermeira me cuida e aprender dessa experiência. Conclusões: Os significados de puérperas com morbidade materna extrema estão carregados de experiências, crenças culturais e sentimentos, onde o cuidado compassivo e congruente de enfermagem ajuda na sua sobrevivência e a internação na UTI constitui-se num ensinamento para a vida.
Structural Performance of Externally Strengthened Rectangular Reinforced Concrete Beams by Glued Steel Plate
Authors: John A. T. , Nwankwo E. , Orumu Solomon Teminusi , Osuji S. O. 
Published: October 2019

This paper examines both flexural and shear behaviour of eight full-scale (2700×160×100-mm) reinforced concrete rectangular beams subjected to one-third point load. Two types of beams were investigated; Type-E and Type-C. Type-E are reinforced concrete rectangular beams strengthened externally by 1.5mm thick structural steel plate glued to the tensile face with epoxy as adhesive while type-C are reinforced concrete rectangular beams without structural steel plate glued to the tensile face. An average concrete strength of 30N/mm2 at 28 days was used. Required internal reinforcement according to BS 8110-1:1997 was provided for the concrete rectangular beams. Before the beams were externally strengthened, the beam surface to be plated was gritted to take off the cement membrane and to open up the aggregates. Epoxy adhesive was applied as a paste to both the plate and concrete surfaces: the two surfaces were then put together and held in place under pressure of 3.84kN/m2 until the glue was cured. The beams were subjected to flexural testing after 28 days, using loading frame. Each of the rectangular beams support at both ends were subjected to one-third point load, deflection readings were recorded using a dial gauge at every 1.82kN increment. At ultimate load, the beams failed by a crack initiated at the bottom fiber of the beams. From the test results, an average flexural and shear strengths of Type-C beams are; 21.91N/mm2 and 1.05N/mm2 respectively, while type-E beams are; 28.91N/mm2 and 1.39N/mm2 respectively. The results of the investigation showed that flexural and shear strengths of reinforced concrete rectangular beam increased when strengthened externally by bonded steel plate. A straightforward analytical procedure was developed to validate the experiment results of type-E and type-C beams, using rectangular stress block for concrete. Experimental average failure load for beams Type-C and Type-E are 22.44kN and 29.60kN respectively while theoretical failure load for Type-C and Type-E beams are 20.86kNand 31.2kN respectively. Generally, there were acceptably fair correlations between analytical and experimental failure loads of Type-C and Type-E beams.
Hardware co-simulation of 1024-point FFT and it's Implementation, in Simulink, Xilinx Vivado IDE on Zynq-7000 FPGA
Authors: Shashidhara K. S. , Srinivasaiah H. C. 
Published: October 2019

In this paper a 1024-point FFT Algorithm is implemented on Zynq-7000 FPGA device. The design implementation uses Hardware co-simulation in Simulink and Xilinx Vivado environments with Zynq-7000 FPGA target evaluation board using JTAG setup. The power parameter for the configured FFT IP core for 1024 point and the signal source DDS block are estimated. The DDS with both sine and cosine signal outputs enabled, consume a power of 0.277 W, whereas, the 1024 point FFT core consume a power of 0.044 W. Further when 2 DDSs were instanced to generate orthogonal sine and cosine sources for OFDM signals of same frequency 1MHz each, a total of 0.277W power is consumed. When a single DDS core is configured for both sine or cosine signal only configuration by instancing a 1024-point FFT core the total power consumed is 0.268W and 0.267W, respectively, a 1mW higher to cosine case. Further, when 1024 point FFT core power alone is calculated it is found to be 0.044W (or 44mW). When a single DDS is instanced for OFDM signal generation by opting both the sine cosine signals, it consumed a total power of 0.233 W saving a power of 0.044W or 44mW by sine or cosine data re-use from the LUT ROM of DDS. Thus saving a power of 44mW by using data re-use through LUT’s of DDS. This is a significant power saving. In this, hardware co-simulation process, Xilinx system generator tool (Sysgen) is used. This implementation is coded using Verilog HDL, verified on Xilinx Vivado platform on the Zynq-7000 FPGA device. Note that Zynq-7000 is supporting hardware co-simulation, hence the 1024-point FFT has been implemented on this device. The simulation results are captured on Xilinx signal viewer for a proper conclusion.