bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of antigen removal on a porcine osteochondral xenograft for articular cartilage repair
Authors: Elder Steve , Chenault Hudson , Gloth Paul , Webb Katie , Recinos Ruth , Wright Emily , Moran Dalton , Butler James , Borazjani Abdolsamad , Cooley Avery 
Published: March 2018

AbstractGiven the limited availability of fresh osteochondral allografts and uncertainty regarding performance of decellularized allografts, this study was undertaken as part of an effort to develop an osteochondral xenograft for articular cartilage repair. The purpose was to evaluate a simple antigen removal procedure based mainly on treatment with SDS and nucleases. Histology demonstrated a preservation of collagenous structure and removal of most nuclei. Immunohistochemistry revealed the apparent retention of α‐Gal within osteocyte lacunae unless the tissue underwent an additional α‐galactosidase processing step. Cytoplasmic protein was completely removed as shown by Western blot. Quantitatively, the antigen removal protocol was found to extract approximately 90% of DNA from cartilage and bone, and it extracted over 80% of glycosaminoglycan from cartilage. Collagen content was not affected. Mechanical testing of cartilage and bone were performed separately, in addition to testing the cartilage–bone interface, and the main effect of antigen removal was an increase in cartilage hydraulic permeability. In vivo immunogenicity was assessed by subcutaneous implantation into DBA/1 J mice, and the response was typical of a foreign body rather than immune reaction. Thus, an osteochondral xenograft produced as described has the potential for further development into a treatment for osteochondral lesions in the human knee. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2251‐2260, 2018.
A MMP7‐sensitive photoclickable biomimetic hydrogel for MSC encapsulation towards engineering human cartilage
Authors: Aisenbrey Elizabeth A. , Bryant Stephanie J. 
Published: March 2018

AbstractCartilage tissue engineering strategies that use in situ forming degradable hydrogels for mesenchymal stem cell (MSC) delivery are promising for treating chondral defects. Hydrogels that recapitulate aspects of the native tissue have the potential to encourage chondrogenesis, permit cellular mediated degradation, and facilitate tissue growth. This study investigated photoclickable poly(ethylene glycol) hydrogels, which were tailored to mimic the cartilage microenvironment by incorporating extracellular matrix analogs, chondroitin sulfate and RGD, and crosslinks sensitive to matrix metalloproteinase 7 (MMP7). Human MSCs were encapsulated in the hydrogel, cultured up to nine weeks, and assessed by mRNA expression, protein production and biochemical analysis. Chondrogenic genes, SOX9, ACAN, and COL2A1, significantly increased with culture time, and the ratios of COL2A1:COL10A1 and SOX9:RUNX2 reached values of ∼20–100 by week 6. The encapsulated MSCs degraded the hydrogel, which was nearly undetectable by week 9. There was substantial deposition of aggrecan and collagen II, which correlated with degradation of the hydrogel. Minimal collagen X was detectable, but collagen I was prevalent. After week 1, extracellular matrix elaboration was accompanied by a ∼twofold increase in compressive modulus with culture time. The MMP7‐sensitive cartilage mimetic hydrogel supported MSC chondrogenesis and promoted macroscopic neocartilaginous matrix elaboration representative of fibrocartilage. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2344‐2355, 2018.
Influence of CuO/MgO ratio on the gene expression, cytocompatibilty, and antibacterial/anticancerous/analgesic drug loading kinetics for (15‐x) CuO‐xMgO‐10P2O5‐60SiO2‐10CaO‐5ZnO (2.5 ≤ x ≤ 12.5) mesoporous bioactive glasses
Authors: Bains Rupinderjeet , Sharma Piyush , Mir Rameez Ahmad , Jeet Suninder , Kaur Gurbinder , Pandey Om Prakash 
Published: March 2018

AbstractIn the present study, novel mesoporous bioactive glasses (MBGs) (15‐x)CuO‐xMgO‐10P2O5‐60SiO2‐10CaO‐5ZnO (2.5 ≤ x ≤ 12.5, varying in steps of 2) are synthesized using the sol‐gel technique. The structural phases of the glasses/glass ceramics were studied by XRD. The pH variation and simulated body fluids (SBF) studies demonstrated the in‐vitro bioactivity of all the MBGs. MBGs possess surface area variation between 98.22 and 442.41 cm2/g. The pore size of MBGs lies in the range of 5.8–8.8 nm. The cytotoxicity assays were conducted for MG63 human osteosarcoma cell line depicting non‐toxic behavior of all MBGs at 7.8125 μg/ml. In addition to this, the effect of the magnesium on the gene expression was also investigated using reverse transcription–polymerase chain reaction (RT‐qPCR). The MBGs were loaded with the antibacterial (vancomycin/amoxicillin), anticancerous (doxorubicin), and analgesic (Iburofen) drugs. Ibuprofen and amoxicillin drugs were almost fully loaded in all the MBGs, whereas doxorubicin and vancomycin drugs illustrated variation in loading with decreasing copper content. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2116‐2130, 2018.
High-Impact Mechanical Loading Increases Bone Material Strength in Postmenopausal Women—A 3-Month Intervention Study
Authors: Sundh Daniel , Nilsson Martin , Zoulakis Michail , Pasco Courtney , Yilmaz Melis , Kazakia Galateia J , Hellgren Martin , Lorentzon Mattias 
Published: March 2018

ABSTRACT

Bone adapts to loading in several ways, including redistributing bone mass and altered geometry and microarchitecture. Because of previous methodological limitations, it is not known how the bone material strength is affected by mechanical loading in humans. The aim of this study was to investigate the effect of a 3-month unilateral high-impact exercise program on bone material properties and microarchitecture in healthy postmenopausal women. A total of 20 healthy and inactive postmenopausal women (aged 55.6 ± 2.3 years [mean ± SD]) were included and asked to perform an exercise program of daily one-legged jumps (with incremental number, from 3×10 to 4×20 jumps/d) during 3 months. All participants were asked to register their performed jumps in a structured daily diary. The participants chose one leg as the intervention leg and the other leg was used as control. The operators were blinded to the participant's choice of leg for intervention. The predefined primary outcome was change in bone material strength index (BMSi), measured at the mid tibia with a handheld reference probe indentation instrument (OsteoProbe). Bone microstructure, geometry, and density were measured with high-resolution peripheral quantitative computed tomography (XtremeCT) at the ultradistal and at 14% of the tibia bone length (distal). Differences were analyzed by related samples Wilcoxon signed rank test. The overall compliance to the jumping program was 93.6%. Relative to the control leg, BMSi of the intervention leg increased 7% or 0.89 SD (p = 0.046), but no differences were found for any of the XtremeCT-derived bone parameters. In conclusion, a unilateral high-impact loading program increased BMSi in postmenopausal women rapidly without affecting bone microstructure, geometry, or density, indicating that intense mechanical loading has the ability to rapidly improve bone material properties before changes in bone mass or structure. © 2018 The Authors. Journal of Bone and Mineral Research Published by Wiley Periodicals Inc.
NOTCH4 is a possible novel susceptibility gene for dilated cardiomyopathy in the Chinese population: A case‐control study
Authors: Shi Xiaoqing , Zhang Yang , Li Bingjie , Peng Mengle , Yuan Yingying , Wang Ximing , Li Xinqiang , Yu Dongze , Li Yongzhe , Qin Dongchun 
Published: March 2018

BackgroundThe incidence of dilated cardiomyopathy (DCM) has increased in recent years, and many studies have sought to further improve the general understanding of this condition. Previous studies have demonstrated that some single nucleotide polymorphisms (SNPs) associated with systemic lupus erythematosus also affect susceptibility to DCM, suggesting that immune‐related diseases may share similar genetic susceptibility. Recent large‐scale and genome‐wide association studies have identified NCR3, NOTCH4, CYP1A2, ITGA1, OPRM1, ST8SIA2, and LINC00704 as genetic risk factors associated with cardiac manifestations of neonatal lupus. Here, we aimed to determine whether these SNPs conferred susceptibility to DCM in the Chinese Han population.MethodsWe investigated the relationship between these polymorphisms and DCM risk in 273 patients with DCM and 548 healthy controls. Genotyping was performed using MassArray iPLEX system.ResultsLogistic regression analysis indicated that the T allele of rs3134942 in NOTCH4 gene increased the risk of DCM by 61% compared with the G allele (Pa = 6.57 × 10−3). The SNP rs3134942 was also significantly associated with increased DCM risk in the additive (Pa = 6.57 × 10−3) and dominant models (Pa = 1.01 × 10−2). Additionally, rs2472299 in CYP1A2 gene showed suggestive association with reduced risk of DCM in the dominant model (Pa = 4.24 × 10−2) and was correlated with smoking status in patients with DCM (Pa = 1.56 × 10−2).ConclusionsOur findings suggested that rs3134942 in NOTCH4 may be involved in DCM risk. Further, studies in larger and ethnically diverse populations are required to confirm the results reported in this study.
Clinical Pharmacokinetics of Sulfobutylether‐β‐Cyclodextrin in Patients With Varying Degrees of Renal Impairment
Authors: Hoover Randall K. , Alcorn Harry , Lawrence Laura , Paulson Susan K. , Quintas Megan , Luke David R. , Cammarata Sue K. 
Published: March 2018

AbstractDelafloxacin, a fluoroquinolone, has activity against Gram‐positive organisms including methicillin‐resistant S aureus and fluoroquinolone‐susceptible and ‐resistant Gram‐negative organisms. The intravenous formulation of delafloxacin contains the excipient sulfobutylether‐β‐cyclodextrin (SBECD), which is eliminated by renal filtration. This study examined the pharmacokinetics and safety of SBECD after single intravenous (IV) infusions in subjects with renal impairment. The study was an open‐label, parallel‐group, crossover study in subjects with normal renal function or mild, moderate, or severe renal impairment, and those with end‐stage renal disease undergoing hemodialysis. Subjects received 300 mg delafloxacin IV or placebo IV, containing 2400 mg SBECD, in 2 periods separated by ≥14‐day washouts. SBECD total clearance decreased with decreasing renal function, with a corresponding increase in area under the concentration‐time curve (AUC0‐∞). After IV delafloxacin 300 mg administration, SBECD mean total clearance was 6.28 and 1.24 L/h, mean AUC0‐∞ was 387 and 2130 h·μg/mL, and mean renal clearance was 5.36 and 1.14 L/h in normal and severe renal subjects, respectively. Similar values were obtained after IV placebo administration. In subjects with end‐stage renal disease, delafloxacin 300 mg IV produced mean SBECD AUC0‐48 values of 2715 and 7861 h·μg/mL when dosed before and after hemodialysis, respectively. Total SBECD clearance exhibited linear relationships to estimated glomerular filtration rate and creatinine clearance. Single doses of IV delafloxacin 300 mg and IV placebo were well tolerated in all groups. In conclusion, decreasing renal function causes reduced SBECD clearance and increased exposures, but SBECD continues to exhibit a good safety and tolerability profile in IV formulations.
Pharmacokinetics and Tolerability of Letermovir Coadministered With Azole Antifungals (Posaconazole or Voriconazole) in Healthy Subjects
Authors: Marshall William L. , McCrea Jacqueline B. , Macha Sreeraj , Menzel Karsten , Liu Fang , van Schanke Arne , de Haes Joanna I. Udo , Hussaini Azra , Jordan Heather R. , Drexel Melissa , Kantesaria Bhavna S. , Tsai Christine , Cho Carolyn R. , Hulskotte Ellen G. J. , Butterton Joan R. , Iwamoto Marian 
Published: March 2018

AbstractLetermovir is a human cytomegalovirus terminase inhibitor for cytomegalovirus infection prophylaxis in hematopoietic stem cell transplant recipients. Posaconazole (POS), a substrate of glucuronosyltransferase and P‐glycoprotein, and voriconazole (VRC), a substrate of CYP2C9/19, are commonly administered to transplant recipients. Because coadministration of these azoles with letermovir is expected, the effect of letermovir on exposure to these antifungals was investigated. Two trials were conducted in healthy female subjects 18 to 55 years of age. In trial 1, single‐dose POS 300 mg was administered alone, followed by a 7‐day washout; then letermovir 480 mg once daily was given for 14 days with POS 300 mg coadministered on day 14. In trial 2, on day 1 VRC 400 mg was given every 12 hours; on days 2 and 3, VRC 200 mg was given every 12 hours, and on day 4 VRC 200 mg. On days 5 to 8, letermovir 480 mg was given once daily. Days 9 to 12 repeated days 1 to 4 coadministered with letermovir 480 mg once daily. In both trials, blood samples were collected for the assessment of the pharmacokinetic profiles of the antifungals, and safety was assessed. The geometric mean ratios (90%CIs) for POS+letermovir/POS area under the curve and peak concentration were 0.98 (0.83, 1.17) and 1.11 (0.95, 1.29), respectively. Voriconazole+letermovir/VRC area under the curve and peak concentration geometric mean ratios were 0.56 (0.51, 0.62) and 0.61 (0.53, 0.71), respectively. All treatments were generally well tolerated. Letermovir did not affect POS pharmacokinetics to a clinically meaningful extent but decreased VRC exposure. These results suggest that letermovir may be a perpetrator of CYP2C9/19‐mediated drug‐drug interactions.
Nodular gastritis in association with gastric cancer development before and after Helicobacter pylori eradication
Authors: Nishikawa Izumi , Kato Jun , Terasoma Satoshi , Matsutani Hiroyoshi , Tamaki Hidehiko , Tamaki Tetsuya , Kuwashima Fumiaki , Nakata Hiroya , Tomeki Tatsuji , Matsunaka Hideyuki , Ibata Yumiko , Yamashita Yasunobu , Maekita Takao , Higashi Katsuhiko , Ichinose Masao 
Published: March 2018

Background and AimNodular gastritis is caused by Helicobacter pylori infection and is associated with the development of diffuse‐type gastric cancer. This study examined the clinical characteristics of patients with nodular gastritis, including cancer incidence before and after H. pylori eradication.MethodsThis was a retrospective study of patients who underwent upper endoscopy and were positive for H. pylori infection. We examined the clinical findings and follow‐up data after H. pylori eradication in patients with and without nodular gastritis.ResultsOf the 674 patients with H. pylori infections, nodular gastritis was observed in 114 (17%). It was more prevalent in women (69%) and young adults. Among patients with nodular gastritis, six (5%) had gastric cancer, all of which were of the diffuse type. Among the 19 (4%) patients with gastric cancer and no nodular gastritis, 16 had intestinal‐type cancer. White spot aggregates in the corpus, a specific finding in patients with nodular gastritis, were more frequently observed in patients with gastric cancer than in those without (83% vs 26%, P = 0.0025). Of 82 patients with nodular gastritis who had H. pylori eradicated successfully, none developed gastric cancer over a 3‐year follow‐up period, while 7 (3%) of 220 patients without nodular gastritis developed gastric cancer after H. pylori eradication.ConclusionsIn patients with nodular gastritis, white spot aggregates in the corpus may indicate a higher risk of developing diffuse‐type gastric cancer. Nodular gastritis may be an indication for eradication therapy to reduce the risk of cancer development after H. pylori eradication.
MicroRNA‐guided gene expression in prostate cancer: Literature and database overview
Authors: Bryzgunova Olga E. , Konoshenko Maria Yu , Laktionov Pavel P. 
Published: March 2018

AbstractInsight into the aberrant expression of microRNAs (miRNAs) and the genes that they regulate during the progression of cancer in general and prostate cancer (PCa) in particular is one of the most important issues in current molecular biomedicine and allows for the discovery of therapeutic or diagnostic miRNA targets. The present study aimed to analyze the available data regarding the direct or indirect effects of miRNAs on the expression of the mRNAs involved in carcinogenesis and to enable updating and optimizing the selection of the corresponding targets. The present review focuses on the data related to the genes with miRNA‐dependent expression during the development of PCa. The data used in this review have been extracted from research papers and the databases STRING, PANTHER and TargetScan, with a special focus on the genes directly associated with cell transformation and the maintenance of the transformed genotype, as well as tumor invasion and spread. The search for miRNA markers of PCa and therapeutically active molecules should rely on bioinformatics resources, such as data from recent experimental studies, as well as meta‐analysis and cross‐analysis of the data on the state of the tumor, patient status, histological/immunohistological data and data on mRNA–miRNA coexpression.
Article alerts: Items from 2013
Authors: Hafdahl Adam R. 
Published: March 2018

This eighth installment of ‘Article Alerts’ includes a print component with 200 methodological articles about research synthesis published in 2013. Since the preceding installment, more than 3400 articles, dissertations, book chapters, and other types of work in this methodological domain have been added to the archive component, all from 2009 to 2013. The online Supporting Information now includes over half of the parent compilation's more than 26 000 records: 1000 from the print component and more than 13 000 from the archive component. Current online addresses for similar bibliographic resources are provided.
Rapid prediction of deoxynivalenol contamination in wheat bran by MOS‐based electronic nose and characterization of the relevant pattern of volatile compounds
Authors: Lippolis Vincenzo , Cervellieri Salvatore , Damascelli Anna , Pascale Michelangelo , Di Gioia Annalisa , Longobardi Francesco , De Girolamo Annalisa 
Published: March 2018

AbstractBACKGROUNDDeoxynivalenol (DON) is a mycotoxin, mainly produced by Fusarium sp., most frequently occurring in cereals and cereal‐based products. Wheat bran refers to the outer layers of the kernel, which has a high risk of damage due to chemical hazards, including mycotoxins. Rapid methods for DON detection in wheat bran are required.RESULTSA rapid screening method using an electronic nose (e‐nose), based on metal oxide semiconductor sensors, has been developed to distinguish wheat bran samples with different levels of DON contamination. A total of 470 naturally contaminated wheat bran samples were analyzed by e‐nose analysis. Wheat bran samples were divided in two contamination classes: class A ([DON] ≤ 400 µg kg−1, 225 samples) and class B ([DON] > 400 µg kg−1, 245 samples). Discriminant function analysis (DFA) classified wheat bran samples with good mean recognizability in terms of both calibration (92%) and validation (89%). A pattern of 17 volatile compounds of wheat bran samples that were associated (positively or negatively) with DON content was also characterized by HS‐SPME/GC–MS.CONCLUSIONSThese results indicate that the e‐nose method could be a useful tool for high‐throughput screening of DON‐contaminated wheat bran samples for their classification as acceptable / rejectable at contamination levels close to the EU maximum limit for DON, reducing the number of samples to be analyzed with a confirmatory method. © 2018 Society of Chemical Industry
An AMOLED pixel circuit for high image quality of 1000 ppi mobile displays in AR and VR applications
Authors: Keum Nack‐Hyeon , Hong Seong‐Kwan , Chai Chong Chul , Kwon Oh‐Kyong 
Published: March 2018

AbstractIn this paper, an active‐matrix organic light‐emitting diode pixel circuit is proposed to improve the image quality of 5.87‐in. mobile displays with 1000 ppi resolution in augmented and virtual reality applications. The proposed pixel circuit consisting of three thin‐film transistors (TFTs) and two capacitors (3T2C) employs a simultaneous emission driving method to reduce the number of TFTs and the emission current error caused by variations in threshold voltage (Vth) and subthreshold slope (SS) of the low‐temperature polycrystalline silicon TFTs. Using the simultaneous emission driving method, the compensation time is increased to 90 μs from 6.5 μs achieved in the conventional six TFTs and one capacitor (6T1C) pixel circuit. Consequently, the emission current error of the proposed 3T2C pixel circuit was reduced to ±3 least significant bit (LSB) from ±12 LSB at the 32nd gray level when the variations in both the Vth and SS are ±4σ. Moreover, both the crosstalk errors due to the parasitic capacitances between the adjacent pixel circuits and due to the leakage current were achieved to be less than ±1 LSB over the entire gray level. Therefore, the proposed pixel circuit is very suitable for active‐matrix organic light‐emitting diode displays requiring high image quality.
A simple, fast, and accurate thermodynamic‐based approach for transfer and prediction of gas chromatography retention times between columns and instruments Part I: Estimation of reference column geometry and thermodynamic parameters
Authors: Hou Siyuan , Stevenson Keisean A. J. M. , Harynuk James J. 
Published: March 2018

AbstractThe transfer of retention times based on thermodynamic models between columns can aid in separation optimization and compound identification in gas chromatography. Although earlier investigations have been reported, this problem remains unsuccessfully addressed. One barrier is poor predictive accuracy when moving from a reference column or system to a new target column or system. This is attributed to challenges associated with the accurate determination of the effective geometric parameters of the columns. To overcome this, we designed least squares‐based models that account for geometric parameters of the columns and thermodynamic parameters of compounds as they partition between mobile and stationary phases. Quasi‐Newton‐based algorithms were then used to perform the numerical optimization. In this first of three parts, the model used to determine the geometric parameters of the reference column and the thermodynamic parameters of compounds subjected to separation is introduced. As will be shown, the overall approach significantly improves the predictive accuracy and transferability of thermodynamic data (and retention times) between columns of the same stationary phase chemistry. The data required for the determination of the thermodynamic parameters and retention time prediction are obtained from fast and simple experiments. The proposed model and optimization algorithms were tested and validated using simulated and experimental data.