bluuebunny/crossref_metadata_2025_split huggingface dataset:
Precocious Chondrocyte Differentiation Disrupts Skeletal Growth in Kabuki Syndrome Mice
Authors: Fahrner Jill A. , Lin Wan-Ying , Riddle Ryan C. , Boukas Leandros , DeLeon Valerie B. , Chopra Sheetal , Lad Susan E. , Luperchio Teresa Romeo , Hansen Kasper D. , Bjornsson Hans T. 
Published: April 2019

AbstractKabuki syndrome 1 (KS1) is a Mendelian disorder of the epigenetic machinery caused by mutations in the gene encoding KMT2D, which methylates lysine 4 on histone H3 (H3K4). KS1 is characterized by intellectual disability, postnatal growth retardation, and distinct craniofacial dysmorphisms. A mouse model (Kmt2d+/ β Geo) exhibits features of the human disorder and has provided insight into other phenotypes; however, the mechanistic basis of skeletal abnormalities and growth retardation remains elusive. Using high-resolution micro-computed tomography we show thatKmt2d+/βGeomice have shortened long bones and ventral bowing of skulls.In vivoexpansion of growth plates within both the skull and long bones suggests disrupted endochondral ossification as a common disease mechanism. Stable chondrocyte cell lines harboring inactivating mutations inKmt2dexhibit increased proliferation and differentiation, which further supports this mechanism. A known inducer of chondrogenesis, SOX9, and its targets show markedly increased expression inKmt2d-/-chondrocytes. By transcriptome profiling, we identifyShox2as a putative KMT2D target. We propose that decreased KMT2D-mediated H3K4me3 atShox2releasesSox9inhibition and thereby leads to enhanced chondrogenesis, providing a novel and plausible explanation for precocious chondrocyte differentiation. Our findings not only provide insight into the pathogenesis of growth retardation in KS1, but also suggest novel therapeutic targets to rescue growth retardation in KS1 and related disorders.
Muscle oxygenation maintained during repeated sprints despite inspiratory muscle loading
Authors: Rodriguez Ramón F. , Townsend Nathan E. , Aughey Robert J. , Billaut François 
Published: April 2019

AbstractA high work of breathing can compromise limb oxygen delivery during sustained high-intensity exercise. However, it is unclear if the same is true for intermittent sprint exercise. This project examined the addition of an inspiratory load on locomotor muscle tissue reoxygenation during repeated-sprint exercise. Ten healthy males completed three experimental sessions of ten 10 s sprints, separated by 30 s of passive rest on a cycle ergometer. The first two sessions were “all-out’ efforts performed without (CTRL) or with inspiratory loading (INSP) in a randomised and counterbalanced order. The third experimental session (MATCH) consisted of ten 10 s work-matched intervals. Tissue saturation index (TSI) and deoxy-haemoglobin (HHb) of the vastus lateralis and sixth intercostal space was monitored with near-infrared spectroscopy. Vastus lateralis reoxygenation (ΔReoxy) was calculated as the difference from peak HHb (sprint) to nadir HHb (recovery). Total mechanical work completed was similar between INSP and CTRL (effect size: −0.18, 90% confidence limit ±0.43), and differences in vastus lateralis TSI during the sprint (−0.01, ±0.33) and recovery (−0.08, ±0.50) phases were unclear. There was also no meaningful difference in ΔReoxy (0.21, ±0.37). Intercostal HHb was higher in the INSP session compared to CTRL (0.42, ±0.34), whilst the difference was unclear for TSI (−0.01, ±0.33). During MATCH exercise, differences in vastus lateralis TSI were unclear compared to INSP for both sprint (0.10, ±0.30) and recovery (−0.09, ±0.48) phases, and there was no meaningful difference in ΔReoxy (−0.25, ±0.55). Intercostal TSI was higher during MATCH compared to INSP (0.95, ±0.53), whereas HHb was lower (−1.09, ±0.33). The lack of difference in ΔReoxy between INSP and CTRL suggests that for intermittent sprint exercise, the metabolic O2demands of both the respiratory and locomotor muscles can be met. Additionally, the similarity of the MATCH suggests that ΔReoxy was maximal in all exercise conditions.
A parallel cell-cycle entry pathway with inverted G1 signaling and alternate point of no return
Authors: Liu Chad , Konagaya Yumi , Chung Mingyu , Daigh Leighton H. , Fan Yilin , Won Yang Hee , Terai Kenta , Matsuda Michiyuki , Meyer Tobias 
Published: April 2019

AbstractCell-cycle entry relies on an orderly progression of signaling events. To start, cells first activate the kinase cyclin D-CDK4/6, which leads to eventual inactivation of the retinoblastoma protein Rb. Hours later, cells inactivate APC/CCDH1and cross the final commitment point. However, many cells with genetically deleted cyclin Ds, which activate and confer specificity to CDK4/6, can compensate and proliferate. Despite its importance in cancer, how this alternate pathway operates and whether wild-type cells use this pathway remain unknown. Here, using single-cell microscopy, we demonstrate that cells with acutely inhibited CDK4/6 enter the cell cycle with slowed and fluctuating cyclin E-CDK2 activity. Surprisingly, in this alternate pathway, the order of APC/CCDH1and Rb inactivation is inverted in both cell lines and wild-type mice. Finally, we show that as a consequence of the signaling inversion, Rb inactivation replaces APC/CCDH1inactivation as the point of no return. Together, we provide molecular characterization of a parallel cell-cycle entry pathway, and reveal temporal plasticity that underlies the G1 regulatory circuit.
COUSIN (COdon Usage Similarity INdex): A normalized measure of Codon Usage Preferences
Authors: Bourret Jérôme , Alizon Samuel , Bravo Ignacio G. 
Published: April 2019

AbstractCodon Usage Preferences (CUPrefs) describe the unequal usage of synonymous codons at the gene, genomic region or genome scale. Numerous indices have been developed to measure the CUPrefs of a sequence. We introduce a normalized index to calculate CUPrefs called COUSIN for COdon Usage Similarity INdex. This index compares the CUPrefs of a query against those of a reference dataset and normalizes the output over a Null Hypothesis of random codon usage. COUSIN results can be easily interpreted, quantitatively and qualitatively. We exemplify the use of COUSIN and highlight its advantages with an analysis on the complete coding sequences of eight divergent genomes, two of them with extreme nucleotide composition. Strikingly, COUSIN captures a hitherto unreported bimodal distribution in CUPrefs in genes in the human and in the chicken genomes. We show that this bimodality can be explained by the global nucleotide composition bias of the chromosome in which the gene resides, and by the precise location within the chromosome. Our results highlight the power of the COUSIN index and uncover unexpected characteristics of the CUPrefs in human and chicken. An eponymous tool written in python3 to calculate COUSIN is available for online or local use.
Membrane Protrusion Formation Mediated by Rho/ROCK Signalling and Modulation of Chloride Flux
Authors: Hori Akiko , Nishide Kenji , Yasukuni Yuki , Haga Kei , Kakuta Wataru , Ishikawa Yasuyuki , Hayes Matthew J , Ohnuma Shin-ichi , Kiyonari Hiroshi , Kimura Kazuhiro , Kondo Toru , Sasai Noriaki 
Published: April 2019

ABSTRACTMembrane protrusion is an important structural property associated with various cellular functions. The pentaspan membrane protein Prominin-1 (Prom1/CD133) is known to be localised to the protrusions and plays a pivotal role in migration and the determination of cellular morphology; however, the underlying mechanisms have been elusive. Here, we demonstrate that Prom1 is sufficient to trigger membrane protrusion formation. Overexpression of Prom1 in the RPE-1 cells triggers multiple long cholesterol-enriched protrusions, independently from actin and tubulin polymerisation. For this protrusion formation, the five amino acid stretch located at the carboxyl cytosolic region is essential. Moreover, the small GTPase Rho and its effector kinase ROCK are essential for this protrusion formation, and the intersection point of active Rho and Prom1 is where the protrusion formation initiates. Importantly, Prom1 causes the chloride ion efflux induced by calcium ion uptake, and protrusion formation is closely associated with the chloride efflux activity. Altogether, this study has elucidated that Prom1 plays critical roles for the membrane morphology and chloride ion flux.
Pangenome analysis reveals genetic isolation inCampylobacter hyointestinalissubspecies adapted to different mammalian hosts
Authors: Costa Daniela , Lévesque Simon , Kumar Nitin , Fresia Pablo , Ferrés Ignacio , Lawley Trevor D. , Iraola Gregorio 
Published: April 2019

AbstractCampylobacter hyointestinalisis an emerging pathogen currently divided in two subspecies:C. hyointestinalissubsp.lawsoniiwhich is restricted to pigs, andC. hyointestinalissubsp.hyointestinaliswhich can be found in a much wider range of mammalian hosts. DespiteC. hyointestinalishas been reported as an emerging pathogen, its evolutionary and host-associated diversification patterns are still vastly unexplored. For this reason, we whole-genome sequenced 13C. hyointestinalissubsp.hyointestinalisstrains and performed a comprehensive comparative analysis including publicly available genomes ofC. hyointestinalissubsp.hyointestinalisandC. hyointestinalissubsp.lawsoniito gain insight into the genomic variation of these differentially-adapted subspecies. Both subspecies are distinct phylogenetic lineages which present a barrier to homologous recombination, suggesting genetic isolation. This is further supported by accessory gene patterns that recapitulate the core genome phylogeny. Additionally,C. hyointestinalissubsp.hyointestinalispresents a bigger and more diverse accessory genome, which probably reflects its capacity to colonize different mammalian hosts unlikeC. hyointestinalissubsp.lawsoniithat is host-restricted. This greater plasticity in the accessory genome ofC. hyointestinalissubsp.hyointestinaliscorrelates to a higher incidence of genome-wide recombination events, that may be the underlying mechanism driving its diversification. Concordantly, both subspecies present distinct patterns of gene families involved in genome plasticity and DNA repair like CRISPR-associated proteins and restriction-modification systems. Together, our results provide an overview of the genetic mechanisms shaping the genomes ofC. hyointestinalissubspecies, contributing to understand the biology ofCampylobacterspecies that are increasingly found as emerging pathogens.
Resolution of the proteome, transcript and ionome dynamics upon Zn re-supply in Zn-deficient Arabidopsis
Authors: Arsova Borjana , Amini Sahand , Scheepers Maxime , Baiwir Dominique , Mazzucchelli Gabriel , Carnol Monique , Bosman Bernard , Motte Patrick , de Pauw Edwin , Watt Michelle , Hanikenne Marc 
Published: April 2019

SummaryRegulation of plant Zn acquisition is poorly understood, while Zn deficiency affects over 2 billion people worldwide. We therefore dissected the dynamic response to changes in Zn supply in Arabidopsis.Hydroponically-grown Zn starved plants were re-supplied with Zn. Subsequent time-resolved sampling strategy allowed concomitant quantification of the dynamics of Zn uptake, microsomal and soluble proteins, and specific transcripts, in space (roots and shoots) and time.Zn accumulates in roots within 10min, but 8h are needed before shoot Zn increases. By 8h, root Zn concentration was ~60% of non-starved plants. Overexpressed root Zn transporters further peaked in 10-30min post re-supply, before reaching a minimum in 120min and 200 ppm Zn. Zn-responding signaling/regulatory molecules include receptor and MAP kinases, calcium signaling proteins, phosphoinositides, G-proteins, COP9 signalosome members, as well as multiple transcription factors.Zn acquisition is a highly controlled dynamic process. Our study identifies novel players in Zn homeostasis and points to cross-talk with other nutrients. It paves the way for directed investigation of so far omitted candidates which dynamically respond to sudden changes in Zn supply but are expressed at similar levels at steady-state Zn deficiency and sufficiency.
Impact of demonetization on diffusion of mobile payment service in India
Authors: Sobti Neharika 
Published: April 2019

PurposeThe purpose of this paper is to explore the antecedents of the behavioral intention and adoption of mobile payment services like m-wallets and m-banking by users in India. This is done by examining the diffusion of mobile payment technology within an extended framework of the Unified Theory of Acceptance and Use of Technology (UTAUT) model. The study attempts to extend the UTAUT model further by introducing three more constructs, namely- perceived cost, perceived risk and demonetization effect and analyzes the impact of demonetization that happened in India from November 8, 2016 to December 30, 2016 on the mobile payment service adoption process. Demonetization event is a case in point to assess whether forced adoption breaks the normal diffusion process or lends support to the same in the long term.Design/methodology/approachA survey was conducted in order to gauge the intention behind the adoption of mobile payment modes by users in India. The questionnaire was administered online solely and 880 responses were received within a period of 20 days from February 3, 2017, to February 23, 2017, using Google Forms as a medium. Usable responses were 640. The study adopted partial least square based structural equation modeling (PLS-SEM) technique to analyze the relation between latent variables: performance expectation, effort expectation, social influence, facilitating conditions, perceived cost, perceived risk, demonetization effect, behavioral intention and usage. For this purpose, SmartPLS3.0 software was used to create path diagrams and calculate estimate the significance of factor loadings using the bootstrap technique.FindingsThe key results indicates that behavioral intention, demonetization and facilitating conditions have a positive and significant impact on the adoption of mobile payment services in India. Overall, Model 3, which was extended UTAUT model, was observed to be a better model in explaining the antecedents of behavioral intention and usage. In addition to UTAUT antecedents, perceived cost and perceived risk proved to have additional explanatory power as antecedents of behavioral intention. Age acts as a moderating variable consistently across three models, implying that younger users give more importance to effortless interface of mobile payment services and get more influenced by peers and society that shapes their intention to use mobile payment services.Originality/valueIt is first of its kind attempt to assess the role of Demonetization in examining the antecedents of behavioral intention and adoption of mobile payment services by users in India under an extended UTAUT model. This study comprehensively examined the impact of forced adoption of mobile payment services by users in India in a natural setting provided by demonetization event that took place in India by conducting a primary survey right itself in the month of February, 2017 to get first hand response from the Indian users.
Reconciling the historical divide between strategy process and strategy content
Authors: Randall Craig , Dent Eric B. 
Published: April 2019

PurposeEarly works in strategic management described strategy process and were quickly followed by aplethoraof strategy content articles focusing on tools, theories, frameworks and models for use in strategizing. Subsequently, strategy research and pedagogy diverged along these lines and the two streams have not been satisfactorily reconciled. As the process incorporates content and content requires process, this paper seeks to answer the question; can some relational consistency and historical reconciliation be developed? The purpose of this paper is to propose a process/content interrelation and a generic model of strategizing.Design/methodology/approachThe authors first identify the opportunity for this integration through the historical development of the two streams. The authors then review contemporary scholarly literature, strategic management textbooks and university syllabi to determine which elements of the strategy process and content are most frequently promulgated.FindingsThe authors discover a generally ubiquitous core of concepts, but great inconsistency in how they are emphasized, linked and/or applied. Beyond these core concepts, faculty syllabi included a wide range of more idiosyncratic content (appearing very infrequently – possibly related to instructor research or interest areas), such as blue ocean or game theory. The authors then propose a 2 × 2 matrix with axes of the level of analysis and stage of activity. The authors provide a populated matrix and discuss the implications of this matrix for future scholarship and teaching.Originality/valueThis paper begins a process of integrating the historical divide between strategy process and strategy content. It provides insights for classroom faculty, historians and practitioners.
Formation of management thought in Russia and early USSR from the 1800s to the 1920s
Authors: Marshev Vadim 
Published: April 2019

PurposeDuring the first quarter of the twentieth century in Russia rapidly developed management thought, generated by many reasons, including socio-economic and political transformations, the results of scientific and practical activities of domestic and foreign experts in management. The purpose of this paper is, first, to acquaint readers with some of factors of the development of the history of Russian Management Thought in nineteenth century and at the beginning of twentieth century and, second, to present the most striking results of the formation of the History of Soviet Management Thought (SMT) in post-revolutionary Russia in the form of the movement of the so-called “The scientific organization of labor” (SOL), including “The scientific organization of managerial labor” (or SOML).Design/methodology/approachThe review and causal analysis of the process of formation of the SMT and historiography of the SMT, a brief description of the institutions of SOL and SOMT and a comparative analysis of little-known works of some Russian authors on management topics of nineteenth century are chosen as research methods.FindingsThe paper emphasizes the action of objectivehistorical inertia(or “non-Markoviness”) of the process of development of managerial thought, manifested, on the one hand, in the stable action of some management paradigms but, on the other hand, in identifying paradigmatic anomalies, in identifying the need for constant development of managerial thought, in the development of sought-after ideas and concepts of management, and even in the institutionalization of applied scientific research in the field of management throughout the country (in the form of SOL and SOML).Originality/valueThe paper attempts to attract the attention of researchers to the little-known Russian and Soviet authors and their little-known works in the field of management thought.
Losing control: the gap in multi-level government reporting
Authors: Caruana Josette , Zammit Kimberly 
Published: April 2019

PurposeThe purpose of this paper is to analyse the relationship between control by the Maltese Central Government on Local Government and the format and basis of budgetary and financial reporting used. The study analyses the role of reporting in agency and fiscal federalism theories.Design/methodology/approachSemi-structured interviews were carried out with the controller (Central Government officials and the National Audit Office), while a survey was carried out with the controlled (Maltese Local Councils).FindingsThe type of reporting used by Maltese Local Councils may be undermining the control that Central Government seeks to exercise on overspending and debt levels. The Local Councils’ financial statements report accrual deficits and increasing liabilities. This overspending appears to slip through Parliamentary scrutiny because the latter approves cash allocations to Local Councils; the financial reports submitted to Parliament do not highlight overspending in cash terms; and the cash budget execution report that should be prepared by Local Councils is not given due importance.Originality/valueCentral Government should be consistent in its policy towards Local Government, which may require more elaborate reporting. This study highlights the importance of aligning the reporting required (top-down) and the reporting presented (bottom-up) – otherwise, control is at stake.
Nitrogen deposition slows down the litter decomposition induced by soil macrofauna in the soil of subtropical forests in China
Authors: Jia Yanyan , Gu Dalu , Wu Chuanwan , Yang Wenfei , Du Xiaofeng , Wen Tinggang , Lv Yanna , Kong Xiangshi , Tian Xingjun 
Published: April 2019

AbstractAs atmospheric nitrogen (N) deposition elevates, extensive researches into the consequences of N deposition on litter decomposition process have been triggered; yet, responses of aboveground macro‐detritivores to atmospheric N deposition and their interactive effects on saprotrophic microorganisms and litter decomposition process are not thoroughly understood. By soil incubation, we assessed the influence of N deposition on the decomposition process of broad leaf (Quercus acutissima) and needle (Pinus massoniana) litter mediated by macro‐detritivore isopods (Armadillidium vulgare) and soil microorganisms. Changes in litter chemical composition (total carbohydrate and N), litter mass loss, soil pH values, soil microbial biomass and the activities of degradative enzymes were determined during a 6‐month laboratory incubation. Results showed that N addition enhanced Q. acutissima litter decomposition in the absence of A. vulgare, but decreased that when A. vulgare presence. N addition had no significant effect on P. massoniana litter decomposition regardless of A. vulgare presence or absence. However, N addition decreased isopod feeding contributions, with litter mass loss of 1.83–2.92 times lower than those of only isopod addition treatments in the two litter types. N addition inhibited soil microbial biomass and enzymatic activities related to N and phenolic metabolism of needle litter when isopods presence. Our findings suggest that N addition likely weakens the feeding activity of soil fauna and slows down the litter decomposition in broad‐leaved forests. This implies that a long‐term consequence of N deposition may induce the soil C accumulation and affect the balance of ecosystem nutrient flux in subtropical broad‐leaved forests.
Four new species of the genusLissonota(Ichneumonidae: Banchinae) from South Korea
Authors: Kang Gyu‐Won , Kolarov Janko , Lee Jong‐Wook 
Published: April 2019

AbstractSouth Korean species ofLissonotaare studied. Just two species of this genus were previously known from South Korea. In the present study 22 species are newly recorded from South Korea. Among them, four species,Lissonota coriaceasp. nov.,L. koreanasp. nov.,L.punctasp. nov. andL.sobaekensis, are described. In this paper, we provided key to South Korean species and descriptions for new species, diagnoses for newly recorded species and digital images.