bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests
Authors: Huang Zehao , Li Na , Rao Kaifeng , Liu Cuiting , Wang Zijian , Ma Mei 
Published: March 2018

Background:
More than 2,000 chemicals have been used in the tannery industry.
Although some tannery chemicals have been reported to have harmful effects on both human health
and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations.

Objective:
This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used
in China.

Materials and Methods:
DNA-damaging effects were measured using the SOS/umu test with
Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were
determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell
lines MGC-803 and A549.

Conclusion:
The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell
assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced
DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism.
Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging
effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural
feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the
chromosome-damaging effects of the four MN-test-positive chemicals.
Bioanalytical Method Development and Validation for the Determination of Vasopressin Receptor Antagonist Conivaptan in Mouse Plasma at NanoLevel and its Pharmacokinetic Application
Authors: Alrabiah Haitham , Bakheit Ahmed , Attia Sabray , Mostafa Gamal A.E. 
Published: March 2018

Background:
Conivaptan inhibits two of vasopressin receptor (vasopressin receptor V1a and
V2). Conivaptan is used for the treatment of hyponatremia, and in some instances, for the treatment of
the heart failure.

Methods:
The present study aimed to develop a simple, sensitive, and accurate HPLC with ultraviolet
detection for the assay of conivaptan (CON) in mouse plasma using bisoprolol as internal standard (IS).
A precipitation procedure was used to extract CON and the IS from the mouse plasma. CON was chromatographically
separated using a C18 analytical column at 25°C. The separation was carried out using
a mixture of phosphate buffer (50 mM): acetonitrile (60: 40, v/v, pH 4.5) with a flow rate of 1.0 mL/min
and detection was performed at 240 nm.

Results:
The assay was validated according to the US Food and Drug (FDA) guidelines. The method
demonstrated linearity over a concentration range of 150 - 2000 ng/mL (correlation coefficient: r 2 =
0.9985). The mean recovery of CON from the mouse plasma was 101.13%. All validation parameters
for CON were within the acceptable range.

Conclusion:
The investigated method has been shown to be suitable for estimating the CON in plasma
samples, and this method is sensitive and highly selective, allowing the estimation of its concentrations
up to the nano-scale. The suggested method was successfully used in a pharmacokinetic study of CON
in mouse plasma.
Tracing Aluminium-based Adjuvants: Their Interactions with Immune Competent Cells and their Effect on Mitochondrial Activity
Authors: Danielsson Ravi , Svensson Andreas , Falkman Peter , Eriksson Håkan 
Published: March 2018

Background:
Studies revealing the immune stimulatory properties of aluminium-based adjuvants (ABAs) have been impaired by the absence of simple and reliable methods of tracing the adjuvants and their effect on biochemical processes upon endocytosis.

Objective:
To verify that labelling of ABAs with lumogallion doesn’t affect the physicochemical properties of the adjuvant; tracing cellular interaction with aluminium adjuvants; explore their effect on metabolic activity upon endocytosis.

Methods:
Physicochemical characterization by Z-potential and size distribution of ABAs labelled with lumogallion. Cellular interactions with ABAs by flow cytometry and confocal microscopy. Metabolic activity explored by measuring transformation of tetrazolium into formazan.

Results:
No or minor change of zeta potential and average particle size of lumogallion labelled aluminium oxyhydroxide, AlO(OH) and aluminium hydroxyphosphate, Al(OH)x(PO4)y. Both phagocytosing and non-phagocytosing leukocytes became associated with ABAs at concentrations expected after in vivo administration of a vaccine. The ABAs were relatively toxic, affecting both lymphocytes and monocytes, and Al(OH)x(PO4)y was more toxic than AlO(OH). Endocytosed aluminium adjuvant particles were not secreted from the cells and remained intracellular throughout several cell divisions. The presence of ABAs increased the mitochondrial activity of the monocytic cell line THP-1 and peripheral monocytes, as based on the transformation of tetrazolium into formazan.

Conclusion:
Lumogallion labelled ABAs is a valuable tool tracing interactions between ABAs and cells. Labelled ABAs can be traced intracellularly and ABAs are likely to remain intracellular for a long period of time. Intracellular ABAs increase the mitochondrial activity and the presence of intracellular Al ions is suggested to cause an increased mitochondrial activity.
Prediktor Kekambuhan Tuberkulosis di Kabupaten Bantul Yogyakarta 2014
Authors: Sukmaningtyas Nooria , Rintiswati Ning , Ahmad Riris Andono 
Published: March 2018

Predictors of tuberculosis recurrence in the district of Bantul PurposeThe purpose of this research was to identify associated factors of TB recurrence. MethodsThe study design was a retrospective cohort. We used TB cases registered in Bantul regency data in 2003-2014 that consisted of 3418 patients. Sampling was conducted by non-random methods with purposive sampling based on inclusion criteria of 904 samples. The variables measured were: age, sex, drug swallowing supervisor, health service facility, socioeconomic and sputum conversion. We used the Cox proportional hazard model for data analysis to determine the rate of occurrence and timing of the occurrence of an event. ResultsMale sex (HR = 0.91; p-value = 0.17) age> 40 years (HR = 1.03; p-value = 0.59), low income (HR = 0.89; p-value = 0.11) were not predictors of TB recurrence, while hospital treatment (HR = 0.81; p-value = 0.00), no sputum conversion (HR = 0.58; p-value = 0.00), treatment failure (HR = 0.49; 95% CI: 0.37-0.64), and treatment of less than 6 months (HR = 0.008; 95% CI: 0.00-0.07) were predictors of TB recurrence. There were 56 (6.18%) of 904 patients who experienced relapse after cured or failed treatment. The average patient relapsed after 17 months of completion of the first treatment. ConclusionHealth facilities, sputum conversion, end-of-treatment status and duration of treatment are pulmonary TB recurrence factors, and as a result can be used to predict TB recurrence in the district of Bantul.
Hyperuricemia and hypertension in primary health care center in Kulon Progo
Authors: Ulfah Nor Anita , Kusnanto Hari , Danawati Cornelia Wahyu 
Published: March 2018

Hyperuricemia and hypertension in Wates primary health care centerPurposeThe purpose of this paper was to determine the relationship between hyperuricemia and hypertension occurrence in Wates primary health care center.MethodA cross-sectional study was conducted using medical records of 220 patients with the age of 18 years or older who met the inclusion criteria in Wates primary health care center in 2015.ResultsThe study found that hyperuricemia was associated with hypertension occurrence in Wates primary health care center in 2015, while age and dyslipidemia were confounders in the hyperuricemia and hypertension relationship. ConclusionIt was found that hyperuricemia had some influence on hypertension occurrence. There are many etiological factors associated with hypertension causing the difficulty of predicting the most common causes of hypertension. A cohort study should be done for further study. Early detection of elevated serum uric acid levels as a risk factor for hypertension needs to be done, in order to prevent hypertension.
Merasa aman karena dianggap normal, kemudahan dan kepasrahan sebagai alasan ibu-ibu memilih persalinan rumah di desa: Studi Kualitatif di Kalimantan Tengah
Authors: Aderi Aderi , Prabandari Yayi Suryo , Triratnawati Atik 
Published: March 2018

Feeling safe due to normal antenatal care, easier to going to health facility, and compliance to God decision as reasons for home delivery choice among village mothers: a qualitative study in Central KalimantanPurposeThe purpose of this paper was to explore mothers’ perceptions of delivery at home in Kartika Bhakti Village, Province of Central Kalimantan. MethodsA case study was conducted involving observations, in-depth interviews and focus group discussion with 7 maternity women, 1 maternity women’s husbands, 1 village midwife, 1 community leader and 1 midwife of the village health post in Kartika Bhakti village, Province of Central Kalimantan.ResultsThe study found six reasons why mothers tended to choose to do delivery at home: barriers to delivery at health facilities; attitudes and preparedness of the midwife to deliver home deliveries; delivery at home benefits; based on the results of the pregnancy examination; not afraid of the risks of delivery; and ready to accept the dangers and risks of delivery.ConclusionThe reasons will be used as a consideration for choosing the birthplace. When the results of a pregnancy examination is good, mothers will tend to choose a home delivery. Furthermore, obstacles in health facilities such as barriers in health centers, geographical, hospital barriers, no postpartum services, and economic abilities are also some of the main reasons to choose to deliver at home. Health education and approaches related to delivery safety need to be improved to change mothers’ perceptions.
Hubungan kepatuhan bidan puskesmas dalam penerapan antenatal care terpadu berkualitas dengan deteksi komplikasi dan penyakit pada ibu hamil
Authors: Sholikhah Siti , Pradjatmo Heru , Hakimi Mohammad 
Published: March 2018

Compliance of midwife primary health center in implementation of the antenatal care integrated quality, disease detection and complications in pregnant womenPurpose This research aimed to determine the relationship of midwife’s compliance in the implementation of the antenatal care (ANC) integrated quality with disease detection and complications in pregnant women.MethodsThis research was an observational study with a retrospective cohort design. The sample was midwives who serve pregnant women in a primary health center, involving 125 midwives. The place of research was in 21 health centers throughout Kulon Progo. Research instruments used a questionnaire, with total sampling. Data analysis used: univariable analysis to determine the distribution of the data, bivariable with chi-square tests, multivariable with linear regression tests with 95% CI and significance of p=0.05, and a qualitative analysis.ResultsMidwives' compliance to the standard operating procedure has a 35% greater chance of detecting complications and illness in pregnant women than the midwife who did not follow standard operating procedure. Not all midwives in primary health care comply with ANC integrated quality.ConclusionThis study contributes to an understanding that compliance of midwives to ANC integrated quality is important as an effort to detect complications in pregnant women. The health office should establish a team for socialization, supervision, and assistance in the implementation of ANC integrated quality.
Community needs analysis on community preparedness related to health aspect in facing flash floods disaster in afdeling Gunung Pasar Jember
Authors: Perwiraningrum Dhyani Ayu , Hidayat Rahmat , Dewi Fatwa Sari Tetra 
Published: March 2018

Community preparedness of Afdeling Gunung Pasang to face flash flood disasterPurposeThis research aimed to determine community needs to develop community preparedness on health aspect by needs awareness surveys to prevent flash flood disaster in Afdeling Gunung Pasang, Suci village, Panti district, in Jember.MethodsThis research was a qualitative study with a phenomenological approach. The main informants were community members of Afdeling Gunung Pasang, and supporting informants were leaders of the community, heads of village offices, community stakeholders (local plantation industry) and disaster management stakeholders from the local government institution in Jember. Data were collected by focus group discussions, in depth interviews, and observation. Data validation used triangulation methods. ResultsMany kinds of needs awareness were found in the community, to develop community preparedness in health aspects. The community needs stimulus to prepare for disasters, such as training emergency response, fulfillment tools, disaster information, and coordination with stakeholders.ConclusionThe community needs to prepare health aspects in the event of disasters and observe dangerous zones in Afdeling Gunung Pasang.
Causes of musculoskeletal complaint on carving crafters in Bantul
Authors: Hidayat Rahmat , Hariyono Widodo , Sutomo Adi Heru 
Published: March 2018

Causes of musculoskeletal complaint on carving crafters in BantulPurposeThe purpose of this study was to determine the factors causing musculoskeletal disorders in furniture carvers in Bantul. These factors are age, length of service, work stress and smoking habit.MethodsA cross-sectional study was conducted by distributing questionnaires to 56 respondents in a furniture carving business in Bantul. The independent variables were age, years of service, work stress, and smoking habits, while the dependent variable was a musculoskeletal disorder. Measurement of musculoskeletal disorder used a questionnaire, and Nordic Body Map while work stress measurement used a work stress indicator questionnaire.ResultsThe study found that there was a significant correlation between age and work stress with musculoskeletal complaints in furniture craftsmen in Bantul, while the working period and smoking habit did not significantly influence the musculoskeletal disorder in furniture crafters in Bantul.ConclusionAge and occupational stress are factors that affect musculoskeletal complaints, while the habit of work and smoking habit did not have a significant effect on the occurrence of musculoskeletal complaints in furniture crafters in Bantul. Work rotation and workspace design need to be done properly to minimize the risk of musculoskeletal problems in furniture crafters in Bantul.
BOUT METHODS OF IMPROVING SAFETY OF THE FACILITY FROM THE IMPACT OF AIR SHOCK WAVE
Authors: Rybakov Аnatoly V. , Ivanov Evgeniy V. , Sedov Dmitry S. , Ivanova Lubov V. 
Published: March 2018

В статье рассмотрен алгоритм методики, позволяющей на основе значений показателя за-щищенности осуществлять поиск рационального решения для защиты опасных производственных объектов от воздействия воздушной ударной волны обычных средств поражения. В основе используемой методики выбора рациональных конструктивных параметров лежит задача оптимизации показателя защищенности. Показана программная реализация методики.
CALCULATION OF LONG-TERM FILTRATION IN A POROUS MEDIUM
Authors: Kuzmina Ludmila I. , Osipov Yuri V. , Vetoshkin Nikita V. 
Published: March 2018

he filtration problem in a porous medium is an important part of underground hydromechanics. Filtration of suspensions and colloids determines the processes of strengthening the soil and creating waterproof walls in the ground while building the foundations of buildings and underground structures. It is assumed that the formation of a deposit is dominated by the size-exclusion mechanism of pore blocking: solid particles pass freely through large pores and get stuck at the inlet of pores smaller than the diameter of the particles. A one-dimensional mathematical model for the filtration of a monodisperse suspension includes the equation for the mass balance of suspended and retained particles and the kinetic equation for the growth of the deposit. For the blocking filtration coefficient with a double root, the exact solution is given implicitly. The asymptotics of the filtration problem is constructed for large time. The numerical calculation of the problem is carried out by the finite differences method. It is shown that asymptotic approximations rapidly converge to a solution with the increase of the expansion order.
Effects of Smoking Versus Nonsmoking on Postprandial Glucose Metabolism in Heavy Smokers Compared With Nonsmokers
Authors: Grøndahl Magnus F. , Bagger Jonatan I. , Lund Asger , Faurschou Annesofie , Rehfeld Jens F. , Holst Jens J. , Vilsbøll Tina , Knop Filip K. 
Published: March 2018

OBJECTIVE
Epidemiological studies suggest that smoking increases the risk of type 2 diabetes. We hypothesized that smoking-derived nicotine and ensuing activation of nicotinic cholinergic receptors in the gastrointestinal tract and the autonomic nervous system would have a detrimental effect on postprandial glucose metabolism and, thus, potentially constitute a link between smoking and the development of type 2 diabetes.

RESEARCH DESIGN AND METHODS
We subjected 11 male heavy smokers to two identical 4-h liquid mixed-meal tests: one with concomitant cigarette smoking (immediately before and after meal intake) and one without smoking. Twelve age-, sex-, and BMI-matched nonsmokers underwent an identical meal test without smoking.

RESULTS
The smokers were characterized by higher fasting plasma concentrations of glucagon compared with the nonsmokers. Among smokers, cigarette smoking before and after the meal significantly reduced postprandial plasma glucose excursions. There were no differences in gut or pancreatic hormone concentrations between the test days in the smoking group, and the responses were similar to those in the control group.

CONCLUSIONS
Our results suggest that smoking in association with meal intake decreases the postprandial plasma glucose concentrations, possibly through decreased gastric emptying, and that elevated fasting glucagon concentrations rather than smoking-induced alterations in postprandial glucose and hormone responses may be associated with the elevated risk of type 2 diabetes in chronic smokers.
STUDI HUBUNGAN KEANEKARAGAMAN MAKROFAUNA TANAH DENGAN KANDUNGAN C-ORGANIK DAN ORGANOPHOSFAT TANAH DI PERKEBUNAN COKELAT (Theobroma cacao L.) KALIBARU BANYUWANGI
Authors: Nurrohman Endrik , Rahardjanto Abdulkadir , Wahyuni Sri 
Published: March 2018

Jenis penelitian yang digunakan adalah penelitian deskriftif kuantitatif. Penelitian ini dilakukan pada bulan FebruariMaret 2015. Tujuan penelitian ini adalah untuk mengetahui keanekaragaman makrofauna tanah, kandungan C-organik dan organophosfat tanah. Metode dalam penelitian ini adalah jebakan (pitt fall trap) untuk mengetahui keanekaragaman makrofauna tanah dan analisis laboratorium untuk mengetahui kandungan C-Organik dan organophosfat tanah. Hasil penelitian menunjukkan bahwa (1) Kepadatan berkisar antara 0,25 ind/m2–4,75 ind/m2, kepadatan relatif berkisar antara 0,002-0,052. Frekuensi berkisar antara 0,16–1,00, frekuensi relatif berkisar antara 0,018–0,115. Indeks keanekaragaman jenis Shannon wiener (H’) kategori keanekaragaman jenis rendah. Hasil Uji C-Organik tanah sangattinggi berarti tanah tergolong tanah yang subur. Organophosfat jenis Carbokfuran sangat tinggi
PEMANFAATAN KOMBINASI KULIT KACANG DENGAN BONGGOL PISANG DAN BIJI NANGKA UNTUK PEMBUATAN PLASTIK BIODEGRADABLE DENGAN PENAMBAHAN GLISEROL
Authors: Asngad Aminah , Amelia Risky , Aeni Nurul 
Published: March 2018

Untuk menyelamatkan lingkungan dari bahaya plastik, saat ini telah dikembangkan plastik biodegradable yang dapat diuraikan kembali oleh mikroorganisme secara alami menjadi senyawa yang ramah lingkungan. Tujuan penelitian ini untuk mengetahui ketahanan tarik dan perpanjangan putus serta uji biodegradabel plastik dari kulit kacang dengan bonggol pisang dan biji nangka dengan penambahan gliserol. Penelitian dilakukan di lab.Biologi FKIP UMS, metode penelitian eksperimen. Rancangan penelitian acak lengkap dengan 2 faktor perlakuan yaitu: Faktor 1 kombinasi perlakuan (K), K1= kacang tanah dan bonggol pisang; K2 = kacang tanah dan biji nangka. Faktor 2 kadar gliserol (G), G 1 =6ml; G 2 = 7ml; G3 =8ml. Analisis yang digunakan deskriptif kualitatif untuk uji ketahanan tarik, perpanjangan putus dan uji biodegradabilitas. Hasil penelitian menunjukkan ketahanan tarik paling kuat, K1G1 yakni 1.38 N/mm2, paling rendahK2G3 yakni 0.66 N/mm2. Perpanjangan putus paling tinggi, K1G3 yakni 21.05 N/mm2, paling rendah K2G1 yakni 17.44 N/mm2. Pada K1G3 memiliki nilai rata-rata biodegradabilitas yang paling tinggi, yaitu 90.38% sedangkan K2G1 yangpaling rendah yaitu 58,84%. Berdasarkan hasil penelitian dapat diambil simpulan ada perbedaan ketahanan tarik dan perpanjangan putus serta hasil uji biodegradabilitas plastik biodegradable dari kulit kacang dengan bonggol pisang dan biji nangka dengan penambahan gliserol.
ISOLASI DAN ANALISIS KANDUNGAN MINYAK ATSIRI PADA KEMBANG LESON
Authors: Pratiwi Ambar , Utami Listiatie Budi 
Published: March 2018

Kembang leson sering dimanfaatkan sebagai aromaterapi pada saat pemulihan bagi pasien karena dipercaya dapat menghilangkan rasa lesu. Komposisi kembang leson sangat beragam, terdiri dari beraneka bunga dan rimpang, serta belum ada kajian mendalam terkait dengan kandungan senyawa terutama minyak atsiri pada kembang leson. Penelitian ini bertujuan untuk mengetahui kandungan minyak atsiri yang terdapat pada kembang leson. Pada penelitian ini dilakukan ekstraksi minyak atsiri dengan metode destilasi Stahl, minyak atsiri yang diperoleh dianalisis kualitatif dan kuantitatif. Analisis kualitatif minyak atsiri meliputi pengamatan organoleptis; sementara analisis kuantitatif minyak atsiri dilakukan dengan kromatograf gas-spektra massa (KG-SM). Minyak atsiri yang diperoleh memiliki bentuk cair, jernih, berwarna kuning kecokelatan, bau aromatis. Rendaman minyak atsiri yang diperoleh sebesar 0,75% (v/b). Analisis KG-SM diperoleh 50 komponen penyusun minyak atsiri, lima komponen minyak atsiri yang memiliki luas area tertinggi yaitu camphene kadarnya sebanyak 1,29% , benzene metil cymene 4,93%, camphor 4,75%, cyclohexanemethanol 7,56% dan curdione 4,83%.
PENGARUH EKSTRAK KULIT BUAH RAMBUTAN (Nephelium lappaceul L.) TERHADAP NEKROSIS SEL HEPAR TIKUS (Ratus novergicus) OBESITAS
Authors: Mahmudah Anisah , Tenzer Amy , Lestari Sri Rahayu 
Published: March 2018

Obesitas saat ini disebut sebagai “sindrom baru dunia” yang memerlukan terapi untuk mengatasinya. Terapi herbal adalah terapi yang paling banyak dilakukan penderita obesitas. Kulit buah rambutan mengandung polifenol yangmempunyai aktivitas antioksidan dan berpotensi untuk dikembangkan sebagai obat terapi obesitas. Hepar dapat terkena efek samping obat karena bertanggung jawab melakukan metabolisme obat, termasuk karena pemberian ekstrak kulit buah rambutan. Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak kulit rambutan (Nephelium lappaceum L.) terhadap nekrosis sel hepar tikus (Ratus norvegicus) obesitas. Penelitian ini merupakan penelitian eksperimental rancangan acak lengkap (RAL) dengan 4 perlakuan. Dua puluh empat tikus jantan strain wistar umur 14 minggu yang mengalami obesitas dikelompokkan secara acak menjadi 4 kelompok, diberi ekstrak kulit buah rambutan melalui sonde lambung dengan dosis 15, 30, dan 60 mg/kgbb dan kelompok kontrol diberi akuades. Setelah 12 minggu, tikus didekapitasi dan dikoleksi organ heparnya untuk dibuatpreparat histologi dengan pewarnaan Hemotoksilin-Eosin (HE). Tiap sayatan diamati dalam 3 lapang pandang, diamati 100 sel hepatosit, dihitung persentase sel yang mengalami nekrosis menggunakan hand tally counter. Analisisdata menggunakan analisis varian tunggal pada taraf kepercayaan 95%, dilanjutkan dengan uji BNT dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa ekstrak kulit buah rambutan yang berpengaruh signifian terhadap nekrosis sel hepar tikus adalah 60 mg/kgbb, yaitu meningkatkan persentase piknosis, karyoreksis, karyolisis, serta total nekrosis