bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuronal BC RNA Transport Impairments Caused by Systemic Lupus Erythematosus Autoantibodies
Authors: Muslimov Ilham A. , Iacoangeli Anna , Eom Taesun , Ruiz Anne , Lee Madisen , Stephenson Stacy , Ginzler Ellen M. , Tiedge Henri 
Published: August 2019

The etiology of the autoimmune disorder systemic lupus erythematosus (SLE) remains poorly understood. In neuropsychiatric SLE (NPSLE), autoimmune responses against neural self-antigens find expression in neurological and cognitive alterations. SLE autoantibodies often target nucleic acids, including RNAs and specifically RNA domains with higher-order structural content. We report that autoantibodies directed against neuronal regulatory brain cytoplasmic (BC) RNAs were generated in a subset of SLE patients. By contrast, anti-BC RNA autoantibodies (anti-BC abs) were not detected in sera from patients with autoimmune diseases other than SLE (e.g., rheumatoid arthritis or multiple sclerosis) or in sera from healthy subjects with no evidence of disease. SLE anti-BC abs belong to the IgG class of immunoglobulins and target both primate BC200 RNA and rodent BC1 RNA. They are specifically directed at architectural motifs in BC RNA 5′ stem–loop domains that serve as dendritic targeting elements (DTEs). SLE anti-BC abs effectively compete with RNA transport factor heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2) for DTE access and significantly diminish BC RNA delivery to synapto-dendritic sites of function.In vivoexperiments with male BALB/c mice indicate that, upon lipopolysaccharide-induced opening of the blood–brain barrier, SLE anti-BC abs are taken up by CNS neurons where they significantly impede localization of endogenous BC1 RNA to synapto-dendritic domains. Lack of BC1 RNA causes phenotypic abnormalities including epileptogenic responses and cognitive dysfunction. The combined data indicate a role for anti-BC RNA autoimmunity in SLE and its neuropsychiatric manifestations.SIGNIFICANCE STATEMENTAlthough clinical manifestations of neuropsychiatric lupus are well recognized, the underlying molecular–cellular alterations have been difficult to determine. We report that sera of a subset of lupus patients contain autoantibodies directed at regulatory brain cytoplasmic (BC) RNAs. These antibodies, which we call anti-BC abs, target the BC RNA 5′ domain noncanonical motif structures that specify dendritic delivery. Lupus anti-BC abs effectively compete with RNA transport factor heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2) for access to BC RNAs. As a result, hnRNP A2 is displaced, and BC RNAs are impaired in their ability to reach synapto-dendritic sites of function. The results reveal an unexpected link between BC RNA autoantibody recognition and dendritic RNA targeting. Cellular RNA dysregulation may thus be a contributing factor in the pathogenesis of neuropsychiatric lupus.
The Caenorhabditis elegans Transgenic Toolbox
Authors: Nance Jeremy , Frøkjær-Jensen Christian 
Published: August 2019

Abstract
The power of any genetic model organism is derived, in part, from the ease with which gene expression can be manipulated. The short generation time and invariant developmental lineage have made Caenorhabditis elegans very useful for understanding, e.g., developmental programs, basic cell biology, neurobiology, and aging. Over the last decade, the C. elegans transgenic toolbox has expanded considerably, with the addition of a variety of methods to control expression and modify genes with unprecedented resolution. Here, we provide a comprehensive overview of transgenic methods in C. elegans, with an emphasis on recent advances in transposon-mediated transgenesis, CRISPR/Cas9 gene editing, conditional gene and protein inactivation, and bipartite systems for temporal and spatial control of expression.
Az Alzheimer-kór molekuláris képi megjelenítése
Authors: Albert Annamária , Borbély Katalin 
Published: August 2019

Abstract: The ever-growing average age of the society significantly increases the occurrence of Alzheimer’s disease. The increased prevalence represents considerable social and economic burden, which urges the development of diagnostic and therapeutic methods in the field. The most common cause of dementia is Alzheimer’s disease, the typical histopathological abnormality of which are well known. The detection of functional changes results in the early diagnosis of the disease, which precedes the morphological changes by years. Positron-emission tomography plays an important role in the demonstration of metabolic changes. The glucose metabolic pattern differs significantly in each clinical form of dementia. The most important β-amyloid-binding radiopharmaceuticals that should be highlighted are [11C]Pittsburgh compound B that is widely used in the research and [18F]florbetapir that is commonly approved in diagnostics. Tracers visualising neurofibrillary tangles consisting of tau protein appeared most recently. The development continues; newer and newer radiopharmaceuticals appear. These tracers play an important role in both the research and the diagnostics. Orv Hetil. 2019; 160(33): 1289–1295.
Early B Cell Factor 1 (EBF1) Regulates Glomerular Development by Controlling Mesangial Maturation and Consequently COX-2 Expression
Authors: Nelson Tracy , Velazquez Heino , Troiano Nancy , Fretz Jackie A. 
Published: August 2019

Significance Statement
The specific mechanisms regulating formation of the glomerular tuft during renal development are largely unknown. A previous study showed the transcription factor Early B cell factor 1 (EBF1) is essential for formation of the glomerular tuft. However, the cell types driving glomerular developmental defects in mice lacking EBF1 were not identified. The authors show that deletion of EBF1 from the glomerular mesangium results in impaired glomerular development, whereas deletion of EBF1 from podocytes does not cause developmental abnormalities. They show that EBF1 in the mesangial cells directs glomerular capillary branching through NFAT activation, and consequently COX-2 expression. This is the first evidence that COX-2 inhibition specifically from the mesangial cells impairs renal development, and furthers our understanding of this essential stage of nephrogenesis.

Background
We recently showed the transcription factor Early B cell factor 1 (EBF1) is essential for the last stages of metanephric development, and that mice globally deficient in EBF1 display impaired maturation of peripheral glomeruli. EBF1 is present within multiple glomerular cell types, including the glomerular mesangium and podocytes.

Methods
To identify which cell type is driving the glomerular developmental defects in the global EBF1 knockout mice, we deleted EBF1 from the mesangium/pericytes (Foxd1-cre) or podocytes (Podocin-cre) in mice.

Results
Deletion of EBF1 from Foxd1 lineage cells resulted in hypoplastic kidneys, poorly differentiated peripheral glomeruli, and decreased proximal tubular mass in the outer cortex. Renal insufficiency was apparent at P21 when proteinuria presents, fibrosis of both the glomeruli and interstitium rapidly progresses, microthrombi appear, and hematuria develops. Approximately half of the Foxd1+, Ebf1
fl/fl mice die before they are 3 months old. Mice with podocyte-targeted deletion of EBF1 exhibited no developmental abnormalities. Mice with Ebf1 deficiency in Foxd1 lineage cells shared characteristics with Ptgs2/COX-2–insufficient models, and mechanistic investigation revealed impaired calcineurin/NFATc1 activation and decreased COX-2 expression. Deletion of COX-2 from the interstitial/mesangial lineage displayed a less severe phenotype than EBF1 deficiency in mice. Overexpressing COX-2 in the EBF1-deficient mice, however, partially restored glomerular development.

Conclusions
The results suggest that EBF1 regulates metanephric development at the last stages of glomerular maturation through its actions in the stromal progenitor (Foxd1+) lineage where it mediates proper regulation of calcineurin/NFAT signaling and COX-2 expression.
Los diputados del parlamento gallego en Facebook
Authors: Rúas Araújo José , Dapena González Borja 
Published: August 2019

El objetivo de este estudio es analizar la presencia y utilización de la red social Facebook por parte de los diputados del Parlamento de Galicia, partiendo de los datos más recientes de audiencias y del repaso de diferentes investigaciones sobre la actividad de los parlamentarios españoles en la red (mails, páginas web, blogs, Facebook y Twitter), y comparando los resultados obtenidos en cada caso.Se trata de una investigación cuantitativa, de carácter exploratorio y longitudinal, a través del rastreo de las cuentas en Facebook de los representantes del Parlamento gallego a lo largo de cinco meses, y del seguimiento diario de la actividad en la red de los diputados, observando las distintas variables y posibilidades ofrecidas en el empleo de esta herramienta por parte de los 75 miembros de la Cámara autonómica que integran el universo de estudio.En concreto, se observaron todas las actualizaciones de estado realizadas, comentarios, enlaces, fotografías y vídeos visibles en el muro de cadaparlamentario, calculando sus medias y ranking de actividad, además de comprobar la lista de amigos, grupos y páginas agregadas o el idioma empleado.Para acceder al Facebook de los diputados gallegos se utilizó la técnica de la simulación, a través de la observación participante, además de la información institucional disponible en la red y los datos complementarios solicitados a los distintos grupos parlamentarios.Los resultados muestran que la evolución en la utilización de Facebook es mayor en la sociedad que en los parlamentarios del Congreso de los Diputados y el Parlamento de Galicia y que, si bien en los representantes gallegos se observa mayor presencia que en los anteriores, la utilización de esta herramienta es muy similar en ambos casos, pues apenas la tercera parte de ellos registran cierta actividad, siendo prácticamente testimonial en la mayoría de los diputados estatales y autonómicos analizados.
A SCALE DEVELOPMENT FOR STUDENT SATISFACTION: A PERCEIVED DIMENSION OF QUALITY EDUCATION
Authors: Paharia Pooja 
Published: August 2019

Purpose of the study: A 22 item Likert scale was developed by using Trochim (2000) procedure to measure the academic leader ’ s impact on student satisfaction, which is ultimately considered a factor contributing to quality education. Such an instrument can be used in further empirical researches to understand the role of academic leaders in student satisfaction.
Methodology: Exploratory in-depth interviews were conducted with 20 academicians from the Central University of Rajasthan for item generation, followed by expert testing done by 10 HR experts. Data were collected from 30 students by employing multistage simple random sampling to ensure validity and reliability. SPSS version 21 was used for calculating corrected inter-item to total correlations (CITC) and t values for finalizing items of the questionnaire.
Main Findings: A pool of 25 items was generated at first stage of qualitative interviews with academicians, at the second stage of expert testing 23 items were retained and 2 items were deleted due to low CITC score and t-value. At third stage of pilot testing, 1 item was deleted and 22 items were retained. The instrument for measuring student satisfaction contains was developed containing 22 items.
Applications of this study: This study can be useful in the educational sector for analyzing quality education. It directs further future work by using exploratory and confirmatory factor analysis on larger sample sizes.
Originality of this study: The instrument is developed purely for measuring the impact of academic leaders rather than any other educational and quality factors filling the research gap, based on academic leaders’ behavior, concern, responses, knowledge and other characteristics having an influence on elevating student satisfaction, which is perceived dimension of quality education.
A NEW ANALYTICAL PROCEDURE TO GRAPHICALLY CHARACTERIZE THE TAPHONOMIC PROPERTIES OF SKELETAL CARBONATES. AN EXAMPLE FROM MIOCENE LIMESTONES OF NEW ZEALAND
Authors: CARON VINCENT , BAILLEUL JULIEN , CHANIER FRANK , MAHIEUX GEOFFROY , JOANNY FRANÇOIS-XAVIER 
Published: August 2019

ABSTRACT
A new graphical representation of the taphonomy of skeletal carbonates observed at the thin-section scale is proposed, demonstrating their utility in drawing information about the effects of early sea-floor processes on the post-mortem fate of fossil hard parts. The graphical representations consist of two-axis diagrams, which integrate the degree of fragmentation, abrasion, bioerosion, and encrustation as primary descriptors of the average state of fossil remains in a Miocene limestone section from southeastern North Island of New Zealand. Numerical values are expressed as percentages that represent the contribution of each factor to alteration (i.e., the four factors represent up to 25% each of the total alteration), the sum of values quantifying the amount of departure from pristine condition. Data are plotted in a cumulative form that reflects synergistic action of sea-floor processes towards hard parts destruction. One important virtue of this graphical representation is that the nature, degree, and variability of taphonomic alteration can be visualized and compared in a single diagram for several grain categories within a sample, and between samples. The proposed scheme is particularly flexible because more than four taphonomic categories can be integrated, independently from the number of alteration classes specific to each category, provided conversion of scores obtained in each taphonomic categories into percentages.
Compiled results of such taphonomic analyses could be used in the future to identify specific depositional conditions, such as hydraulic regime, transportation, and residence time on the sea floor (a potential proxy to net accumulation rates).
The effects of oral vitamin D supplementation on the prevention of peritoneal dialysis-related peritonitis: study protocol for a randomized controlled clinical trial
Authors: Zhang Yuhui , Xu Xiao , Pi Haichen , Yang Zhikai , Johnson David W , Dong Jie 
Published: August 2019

Abstract
Background: Vitamin D deficiency has been shown to be closely associated with peritoneal dialysis (PD)-related peritonitis. The aim of this study is to examine the feasibility of conducting a large, powered randomized controlled trial to determine the effects of vitamin D supplementation on the risk of PD-related peritonitis in patients who have already experienced an episode of peritonitis.
Methods: This prospective, open-label randomized controlled pilot trial with blinded end-points (PROBE) aims to determine the feasibility of oral vitamin D supplementation and to explore its effects on the risk of subsequent PD-related peritonitis among PD patients who have recovered from a recent episode of peritonitis. Eligible patients will be randomized 1:1 to either oral vitamin D supplementation (2000 IU per day; intervention group) or no vitamin D supplementation (control group) in addition to usual care according to International Society for Peritoneal Dialysis (ISPD) Guidelines. The sample size will be 30 for both groups. All participants will be followed for 12 months. The primary outcome is the assessment of feasibility (recruitment success, retention, adherence, safety) and fidelity (change in serum 25-hydroxy vitamin D level during follow-up) for a large, powered randomized controlled trial to determine the effects of vitamin D on the risk of PD-related peritonitis in the future. Secondary outcomes include time to peritonitis occurrence, recovery of peritonitis, peritonitis-related transition to hemodialysis, and peritonitis-related death (defined as death within 30 days of peritonitis onset).
Discussion: This is the first randomized controlled trail investigating the effects of vitamin D supplementation on the risk of subsequent PD-related peritonitis among patients on PD. The findings for this pilot study will determine the feasibility of conducting a full-scale randomized controlled trail, which may provide a new strategy for preventing PD-related peritonitis among PD patients.
Trial registration: Clinicaltrails.gov, NCT03264625, registered on 29 August 2017. https://www.clinicaltrials.gov/ct2/show/NCT03264625?term=NCT 03264625&rank=1, assessed on 1 July 2019.
Comparing the effectiveness of magnesium oxide and naldemedine in preventing opioid-induced constipation: A proof of concept, single institutional, two arm, open-label, phase II, randomized controlled trial: the MAGNET study
Authors: Ozaki Anna , Kessoku Takaomi , Iwaki Michihiro , Kobayashi Takashi , Yoshihara Tsutomu , Kato Takayuki , Honda Yasushi , Ogawa Yuji , Imajo Kento , Higurashi Takuma , Yoneda Masato , Taguri Masataka , Yamanaka Takeharu , Ishiki Hiroto , Saito Satoru , Ichikawa Yasushi , Nakajima Atsushi 
Published: August 2019

Abstract
Background: Patients taking opioids are known to develop opioid-induced constipation (OIC), which reduces their quality of life (QOL). The aim of this study is to compare magnesium oxide and naldemedine to determine which is more effective in preventing OIC.
Methods: This is a proof of concept, prospective, randomized controlled trial, conducted in Japan in March 2018. Initially, a questionnaire survey will be carried out targeting adult cancer patients who had concomitantly commenced opioid and OIC prevention treatment. Patients will then be randomly allocated into a magnesium oxide (500 mg, tid) group or a naldemedine (0.2 mg, sid) group. Each drug will be orally administrated for 12 weeks. The primary endpoint is defined as any improvement in the Japanese version of Patient Assessment of Constipation Quality of Life (JPAC-QOL) scores from the baseline to 2 weeks of treatment.
Discussion: The primary endpoint is changes in the JPAC-QOL scores from the baseline to 2 weeks of intervention. The key secondary endpoint will be changes in spontaneous bowel movements (SBMs) at 2 and 12 weeks of intervention. This study will determine whether magnesium oxide or naldemedine is more effective for the prevention of OIC.Trial registration: This trial is registered in the University Hospital Medical Information Network (UMIN) Clinical Trials Registry (UMIN000031891). Registered 25 March 2018, https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000036408.
Ambivalence among women who gave birth after receiving a negative result on non-invasive prenatal testing: a qualitative study
Authors: Yotsumoto Junko , Sekizawa Akihiko , Inoue Satomi , Suzumori Nobuhiro , Samura Osamu , Yamada Takahiro , Miura Kiyonori , Masuzaki Hideaki , Sawai Hideaki , Murotsuki Jun , Hamanoue Haruka , Kamei Yoshimasa , Endo Toshiaki , Fukushima Akimune , Katagiri Yukiko , Takeshita Naoki , Ogawa Masaki , Okamoto Yoko , Tairaku Shinya , Kaji Takashi , Maeda Kazuhisa , Nishizawa Haruki , Matsubara Keiichi , Ogawa Masanobu , Osada Hisao , Ohba Takashi , Kawano Yukie , Sasaki Aiko , Sago Haruhiko 
Published: August 2019

Abstract
Background
This study aimed to investigate the factors affecting ambivalent feelings among women who gave birth after having received negative results on non-invasive prenatal genetic testing (NIPT).
Methods
A questionnaire was sent to women who received a negative NIPT result, and a contents analysis was conducted for those 1562 women who responded to the open-ended question. The contents of these qualitative data were analyzed using the N-Vivo software package.
Results
Environmental factors, genetic counseling-related factors, and increased anticipatory anxiety affected the feeling of ambivalence among pregnant women. Furthermore, pregnant women desired more information regarding the detailed prognosis for individuals with Down syndrome and living with them and/or abortion, assuming the possibility that they were positive.
Conclusions
Three major interrelated factors affected the feeling of ambivalence in women.　Highlighting and discussing such factors during the genetic counseling may resolve some of these ambivalences, thereby enhancing the quality of decisions made by pregnant women.
The association between symptoms of depression during pregnancy and low birth weight:a prospective study
Authors: Li Xiu xiu , Gao Rui , Dai Xiaowei , Liu Hong , Zhang Jinxin , Liu Xuemei , Si Dongmei , Deng Te , Xia Wei 
Published: August 2019

Abstract
BackgroundTo identify whether prenatal depression is a risk factor of low birth weight (LBW) in a Chinese population.Methods1377 singleton pregnant women were recruited from Nanshan Maternity & Child Healthcare Hospital of Shenzhen in this prospective cohort study. Depression scales were assessed by the Edinburgh postnatal depression scale (EPDS) questionnaire in the second trimester of gestation; cut-points for the indication of prenatal depression were ≥12 scores in this study. Sociodemographic data, life-style and pregnancy outcomes were collected through Shenzhen Maternity & Child Healthcare database. The risks of adverse outcomes in patients with depression were determined by multivariate logistic regression and represented as odds ratios (ORs) and 95% confidence intervals (CIs).ResultsOf 1377 subjects, the prevalence of prenatal depression was 19.1%. The EPDS scores were 14.7±7.8and6.5±2.9 (P<0.001) in subjects with and without prenatal depression, respectively. After adjustment for maternal age, education, parity, pre-pregnancy body mass index (BMI), residential area, passive smoking conditions,fetal gender and other confounders, an EPDS score≥12 (versus. <12) was associated withanincreased risk for LBW (odds ratio: 2.05, 95% CI: 1.12-4.64).ConclusionPregnant women presenting antenatal depressive symptoms are at elevated risk of low birth weight.
Association between maternal sleep duration and quality and the risk of preterm birth: a systematic review and meta-analysis of observational studies
Authors: wang ling , Jin Feng 
Published: August 2019

Abstract
Abstract
Background: The association between sleep duration and quality and the risk of preterm birth remains controversial.
Methods: Relevant studies were retrieved from the PubMed and Web of Science databases up to the end of September 2018 and the reference lists of the retrieved articles were reviewed. Random effects models were applied to estimate summarized relative risks (RRs) and 95% confidence intervals (CIs).
Results: Ten identified studies (nine cohort studies and one case-controlled study) examined the associations between sleep duration and quality and the risk of preterm birth. Compared with women with the shortest sleep duration, the summary RRs were 0.76 (95% CI = 0.64–0.89) for women with the longest sleep duration, without between-study heterogeneity (I2 = 0%). Additionally, as compared with women with good sleep quality, the summary RRs were 1.54 (95% CI = 1.18–2.01) for women with poor sleep quality (Pittsburgh Sleep Quality Index > 5), with high between-study heterogeneity (I2 = 76.7%). The results of stratified analysis by trimester, geographical location, study design, type of preterm birth, and adjustment for potential confounders were comparable to those of the main meta-analysis. Funnel plots as well as the Egger’s and Begg’s tests showed no evidence of publication bias.
Conclusions: This systematic review and meta-analysis revealed that short sleep duration and poor sleep quality may be associated with an increased risk of preterm birth.