bluuebunny/crossref_metadata_2025_split huggingface dataset:
LeuRS can leucylate type I and type II tRNALeus in Streptomyces coelicolor
Authors: Fan Jia-Yi , Huang Qian , Ji Quan-Quan , Wang En-Duo 
Published: May 2019

Abstract
Transfer RNAs (tRNAs) are divided into two types, type I with a short variable loop and type II with a long variable loop. Aminoacylation of type I or type II tRNALeu is catalyzed by their cognate leucyl-tRNA synthetases (LeuRSs). However, in Streptomyces coelicolor, there are two types of tRNALeu and only one LeuRS (ScoLeuRS). We found that the enzyme could leucylate both types of ScotRNALeu, and had a higher catalytic efficiency for type II ScotRNALeu(UAA) than for type I ScotRNALeu(CAA). The results from tRNA and enzyme mutagenesis showed that ScoLeuRS did not interact with the canonical discriminator A73. The number of nucleotides, rather than the type of base of the variable loop in the two types of ScotRNALeus, was determined as important for aminoacylation. In vitro and in vivo assays showed that the tertiary structure formed by the D-loop and TψC-loop is more important for ScotRNALeu(UAA). We showed that the leucine-specific domain (LSD) of ScoLeuRS could help LeuRS, which originally only leucylates type II tRNALeu, to aminoacylate type I ScotRNALeu(CAA) and identified the crucial amino acid residues at the C-terminus of the LSD to recognize type I ScotRNALeu(CAA). Overall, our findings identified a rare recognition mechanism of LeuRS to tRNALeu.
Sequence motifs recognized by the casposon integrase of Aciduliprofundum boonei
Authors: Béguin Pierre , Chekli Yankel , Sezonov Guennadi , Forterre Patrick , Krupovic Mart 
Published: May 2019

AbstractCasposons are a group of bacterial and archaeal DNA transposons encoding a specific integrase, termed casposase, which is homologous to the Cas1 enzyme responsible for the integration of new spacers into CRISPR loci. Here, we characterized the sequence motifs recognized by the casposase from a thermophilic archaeon Aciduliprofundum boonei. We identified a stretch of residues, located in the leader region upstream of the actual integration site, whose deletion or mutagenesis impaired the concerted integration reaction. However, deletions of two-thirds of the target site were fully functional. Various single-stranded 6-FAM-labelled oligonucleotides derived from casposon terminal inverted repeats were as efficiently incorporated as duplexes into the target site. This result suggests that, as in the case of spacer insertion by the CRISPR Cas1–Cas2 integrase, casposon integration involves splaying of the casposon termini, with single-stranded ends being the actual substrates. The sequence critical for incorporation was limited to the five terminal residues derived from the 3′ end of the casposon. Furthermore, we characterize the casposase from Nitrosopumilus koreensis, a marine member of the phylum Thaumarchaeota, and show that it shares similar properties with the A. boonei enzyme, despite belonging to a different family. These findings further reinforce the mechanistic similarities and evolutionary connection between the casposons and the adaptation module of the CRISPR–Cas systems.
Disordered region of H3K9 methyltransferase Clr4 binds the nucleosome and contributes to its activity
Authors: Akoury Elias , Ma Guoli , Demolin Segolene , Brönner Cornelia , Zocco Manuel , Cirilo Alexandre , Ivic Nives , Halic Mario 
Published: May 2019

Abstract
Heterochromatin is a distinctive chromatin structure that is essential for chromosome segregation, genome stability and regulation of gene expression. H3K9 methylation (H3K9me), a hallmark of heterochromatin, is deposited by the Su(var)3-9 family of proteins; however, the mechanism by which H3K9 methyltransferases bind and methylate the nucleosome is poorly understood. In this work we determined the interaction of Clr4, the fission yeast H3K9 methyltransferase, with nucleosomes using nuclear magnetic resonance, biochemical and genetic assays. Our study shows that the Clr4 chromodomain binds the H3K9me3 tail and that both, the chromodomain and the disordered region connecting the chromodomain and the SET domain, bind the nucleosome core. We show that interaction of the disordered region with the nucleosome core is independent of H3K9me and contributes to H3K9me in vitro and in vivo. Moreover, we show that those interactions with the nucleosome core are contributing to de novo deposition of H3K9me and to establishment of heterochromatin.
Uncertainty in the Relationship Between Sagittal Alignment and Patient-Reported Outcomes
Authors: Angevine Peter D , Bray David , Cloney Michael , Malone Hani 
Published: May 2019

Abstract

BACKGROUND
Previous studies have reported correlations and precise quantitative relationships between sagittal alignment and health-related quality-of-life (HRQOL) scores. These studies have not reported the extent of uncertainty in these relationships.

OBJECTIVE
To explore the uncertainty in the overall relationships between sagittal alignment and HRQOL and in the predictions of individual patient pain and disability.

METHODS
A retrospective analysis of all new adult patients with long-cassette radiographs and complete outcomes questionnaires presenting to the senior author from 2012 to 2014 was performed. Univariable maximum a posteriori linear regression analyses using Bayesian methods were performed. High-density probability intervals for mean regression relationships and for individual values were calculated using minimally informative prior distributions.

RESULTS
A total of 134 patients satisfied inclusion criteria and were included. For Oswestry Disability Index (ODI) vs pelvic incidence-lumbar lordosis (LL), the 90% high-density probability interval ranged from –0.04 to 0.23, indicating that both the magnitude and direction of the relationship were uncertain. For both ODI vs sagittal vertical axis and ODI vs LL, there was uncertainty in the magnitude of the slope. Wide regions of uncertainty were also seen for predicting individual patient scores.

CONCLUSION
We report the previously unpublished degree of uncertainty in the mean quantitative relationships between radiographic sagittal alignment and patient-reported outcomes and in individual patient outcomes scores. Based on these results, establishing treatment thresholds or predicting an individual's outcome is unreliable. Further research efforts should be focused on developing multilevel hierarchical models incorporating parameter uncertainty and heterogeneous effects.
Prognostic Factors for Adjacent Segment Disease After L4-L5 Lumbar Fusion
Authors: Maragkos Georgios A , Atesok Kivanc , Papavassiliou Efstathios 
Published: May 2019

Abstract

BACKGROUND
Adjacent segment disease (ASD) is an important consideration during decision making for lumbar spinal fusion.

OBJECTIVE
To identify risk factors for development of ASD after L4-L5 fusion and differences in incidence between rostral and caudal ASD.

METHODS
We retrospectively reviewed all consecutive patients at a single institution who underwent first-time spinal fusion at the L4-L5 level for degenerative spinal disease over a 10-yr period, using posterolateral pedicular screw fixation with or without posterior interbody fusion. ASD was defined as clinical and radiographic evidence of degenerative spinal disease requiring reoperation at the level rostral (L3-L4) or caudal (L5-S1) to the index fusion.

RESULTS
Among 131 identified patients, the incidence of ASD requiring reoperation was 25.2% (n = 33). Twenty-four cases (18.3% of the entire cohort) developed rostral ASD (segment L3-L4), 3 cases (2.3%) developed caudal (L5-S1), and 6 cases (4.6%) developed bilateral ASD (both rostral and caudal). Cumulatively, the incidence of caudal ASD was significantly lower than rostral ASD (P < .001). Following multivariate logistic regression for factors associated with ASD reoperation, decompression of segments outside the fusion construct was associated with higher ASD rates (odds ratio [OR] = 2.68, P = .039), as was female gender (OR = 3.55, P = .011), whereas older age was associated with lower ASD incidence (OR = 0.95, P = .011).

CONCLUSION
When considering posterior L4-L5 fusion, surgeons should refrain from prophylactic procedures in the L5-S1 level, without clinical indications, because ASD incidence on that segment is reassuringly low.
Hourly Effect of Pretreatment With IV Antibiotics on Blood Culture Positivity Rate in Emergency Department Patients
Authors: Rand Kenneth H , Beal Stacy G , Rivera Kimberly , Allen Brandon , Payton Thomas , Lipori Gloria P 
Published: May 2019

Abstract
Although it is intuitive that antibiotics administered before obtaining a blood culture would reduce the likelihood of obtaining a positive culture, it is not clear exactly how rapidly and to what extent blood becomes sterile after administration of intravenous (IV) antibiotics. Using a large data set of patients admitted from the UFHealth Shands Adult Emergency Department (ED) between 2012 and 2016 (n = 25 686), we had the opportunity to more closely examine the effect of starting IV antibiotics before vs after obtaining blood cultures. We present data on the effect of pretreatment with IV antibiotics for both septic and nonseptic ED patients on the blood culture positivity rate on an hour-by-hour basis, as well as the effects on distribution of species recovered and the impact of antibiotic resistance in empiric treatment with antibiotics.
Clinical characteristics of cancer-associated myositis complicated by interstitial lung disease: a large-scale multicentre cohort study
Authors: Kaneko Yuko , Nunokawa Takahiro , Taniguchi Yoshinori , Yamaguchi Yukie , Gono Takahisa , Masui Kenichi , Kawakami Atsushi , Kawaguchi Yasushi , Sato Shinji , Kuwana Masataka ,   JAMI investigators, Okano Yutaka , Nishina Naoshi , Tamura Maasa , Kirino Yohei , Ikeda Kei , Kikuchi Jun , Kubo Makoto , Tanino Yoshinori , Kaieda Shinjiro , Naniwa Taio , Watanabe Masaki , Harada Tatsuhiko , Ukichi Taro , Kazuyori Taisuke , Kameda Hideto , Kaburaki Makoto , Matsuzawa Yasuo , Yoshida Shunji , Yoshioka Yasuko , Hirai Takuya , Asakawa Katsuaki , Wada Yoko , Ishii Koji , Fujiwara Sakuhei , Saraya Takeshi , Morimoto Kozo , Hara Tetsu , Suzuki Hiroki , Shibuya Hideki , Muro Yoshinao , Aki Ryoichi , Shibayama Takuo , Ohshima Shiro , Yasuda Yuko , Terada Masaki , Kawahara Yoshie 
Published: May 2019

Abstract

Objective
To clarify the incidence, risk factors, and impact of malignancy in patients with PM/DM-associated interstitial lung disease (ILD).

Methods
This study used data from 497 patients with PM/DM-associated ILD enrolled in a multicentre, retrospective and prospective cohort of incident cases. Cancer-associated myositis (CAM) was defined as malignancy diagnosed within 3 years before or after PM/DM diagnosis. Demographic and clinical information was recorded at the time of diagnosis, and data about the occurrence of mortality and malignancy was collected.

Results
CAM was identified in 32 patients with PM/DM-associated ILD (6.4%). Patients with CAM were older (64 vs 55 years, P &lt; 0.001), presented with arthritis less frequently (24% vs 49%, P = 0.01), and showed a lower level of serum Krebs von den Lungen-6 (687 vs 820 IU/l, P = 0.03) than those without CAM. The distribution of myositis-specific autoantibodies, including anti-melanoma differentiation–associated gene 5, anti-aminoacyl tRNA synthetase, and anti-transcriptional intermediary factor 1-γ antibodies, did not differ between the groups. Survival analysis demonstrated that CAM patients had a poorer survival than non-CAM patients (P = 0.006), primarily due to excess deaths by concomitant malignancy, while mortality due to ILD-related respiratory failure was similar between the groups (P = 0.51).

Conclusion
Concomitant malignancy can occur in patients with PM/DM-associated ILD, and has significant impact on mortality. Older age, lack of arthritis, and a lower level of serum Krebs von den Lungen-6 at diagnosis are predictors of concomitant malignancy.
RESULTS AND DISCUSSION ON JAPANESE PUBLIC OPINION SURVEYS (2006–17) ABOUT NUCLEAR AND RADIATION APPLICATIONS
Authors: Iimoto Takeshi , Takashima Ryuta , Kimura Hiroshi , Kawakami Kazuhisa , Endo Hironori , Yasuda Hiroshi , Nagata Natsuki , Sakai Noriaki , Kawasaki Yumiko , Funakoshi Makoto 
Published: May 2019

Abstract
Public opinion on the application of nuclear technology and radiation could change when a nuclear related event occurs. Japan Atomic Energy Relations Organization has tracked its variation through a nationwide opinion survey in Japan by almost the same way every year since FY 2006. We can identify a continuous long-term fluctuation of Japanese opinion before and after the TEPCO Fukushima Daiichi nuclear disaster using the data. In this study we focused on the trends of public opinion for nuclear energy, impressions and knowledge on radiation, and zero-risk request. For example, radiation can be recognised that it is dangerous and complicated matter by Japanese public regardless of that accident. However, a big change of opinions on radiation was shown on the impression for the word of ‘Useful’ between before and after the accident.
Tafenoquine for primary and terminal prophylaxis of malaria in apparently healthy people: a systematic review
Authors: Rodrigo Chaturaka , Rajapakse Senaka , Fernando Sumadhya Deepika 
Published: May 2019

Abstract
Primaquine was the only licenced antimalarial hypnozoiticidal drug available until recently. Now there is a newly approved alternative: tafenoquine. This review explores the efficacy of tafenoquine as a primary and terminal prophylactic agent in malaria. Multiple databases (Cochrane Central Register of Controlled Trials [CENTRAL], MEDLINE [PubMed], Embase [Ovid], Scopus, CINAHL [EBSCOhost] and LILACS) were searched for clinical randomised controlled trials that had used tafenoquine for prophylaxis without language or time restrictions. The last date of searching was 13 August 2018. For primary prophylaxis, tafenoquine reduced episodes of malaria compared with placebo, at a dose range from 50 mg weekly to 400 mg monthly in three trials conducted in Ghana, Kenya and Thailand. Two trials compared tafenoquine vs mefloquine, but malaria episodes were too few to reach a conclusion. For terminal prophylaxis, evidence from two trials suggest that tafenoquine may have equal or better efficacy compared with primaquine. All trials excluded pregnant participants or those with G6PD deficiency. Tafenoquine is effective for both primary and terminal prophylaxis. If used for primary prophylaxis it may continue to offer protection against vivax relapses after exposure has ended (as terminal prophylaxis).
Paying More for Less: Why Don’t Households in Tanzania Take Advantage of Bulk Discounts?
Authors: Dillon Brian , De Weerdt Joachim , O’Donoghue Ted 
Published: May 2019

Abstract
Do poor households shop in a way that leaves money on the table? A simple way to maximize consumption, conditional on available cash, is to avoid regularly purchasing small amounts of nonperishable goods when bulk discounts are available at modestly larger quantities. Using two-week transaction diaries covering 48,501 purchases by 1,493 households in Tanzania, this article finds that through bulk purchasing the average household could spend 8.7 percent less without reducing purchasing quantities. Several explanations for this pattern are investigated, and the most likely mechanisms are found to be worries about over-consumption of stocks and avoidance of social taxation. Contrary to prior work, there is little indication that liquidity constraints prevent poorer households in the sample from buying in bulk, possibly because the bulk quantities under examination are not very large.
A Case of Cutaneous Arteritis Presenting as Infiltrated Erythema in Eosinophilic Granulomatosis With Polyangiitis: Features of the Unique Morphological Evolution of Arteritis as a Diagnostic Clue
Authors: Shiiyama Rie , Chen Ko-Ron , Ishibashi Masafumi 
Published: May 2019

Abstract:
Eosinophilic granulomatosis with polyangiitis (EGPA, formerly Churg–Strauss syndrome) is a rare systemic vasculitis affecting small- and medium-sized vasculature, associated with asthma and eosinophilia. Different levels of vasculitis in cutaneous lesions have been observed, including dermal small vessel vasculitis and subcutaneous muscular vessel vasculitis. Although the EGPA-associated small vessel vasculitis described as leukocytoclastic vasculitis can be often found in the documented literature, the features of subcutaneous muscular vessel vasculitis in EGPA-associated cutaneous lesions have been rarely demonstrated clinically and histopathologically in English literature. Herein, we report a case of EGPA involving infiltrated erythema on the extremities, with different stages of cutaneous arteritis characterized by eosinophilic arteritis and granulomatous arteritis in the same affected artery. We present this as a unique diagnostic clue for EGPA.
A Comparative Trial of Incisional Negative-Pressure Wound Therapy in Abdominoplasty
Authors: Abesamis Gerald M. , Chopra Shiv , Vickery Karen , Deva Anand K. 
Published: May 2019

Summary:
The risk for minor local complications for abdominoplasty remains high despite advances in strategies in recent years. The most common complication is the formation of seroma with reported rates ranging from 15% to 40%. The use of incisional negative-pressure wound therapy (iNPWT) on closed surgical sites has been shown to decrease the infection, dehiscence, and seroma rates. Thus, this article aims to determine whether an iNPWT dressing, Prevena Plus, is able to reduce postoperative drainage and seroma formation in patients who undergo abdominoplasty. Sixteen consecutive patients who underwent abdominoplasty by a single surgeon were dressed with standard dressings and iNPWT dressings. Total drain output, day of drain removal, and adverse events were compared between cohorts with a minimum follow-up of 6 months. The iNPWT group demonstrated a significantly less amount of fluid drainage with a mean total fluid output of 370 ± 275 ml compared to 1269 ± 436 ml mean total drainage from controls (P < 0.001). Time before removal of both drains was almost halved in the iNPWT group with an average of 5.3 ± 1.6 days, which was significantly less than the average time of 10.6 ± 2.9 days seen in control patients (P < 0.001). No observed adverse events were recorded in either group. Our findings show that iNPWT for a closed abdominoplasty incision decreases the rate of postoperative fluid accumulation and results in earlier drain removal.
Periorbital Aesthetic Surgery: A Simple Algorithm for the Optimal Youthful Appearance
Authors: Akşam Ersin , Karatan Berrak 
Published: May 2019

Background:
Blepharoplasty is one of the most common procedures performed. To achieve optimal results in periorbital rejuvenation, a systematic approach to patients that addresses all the problems in the periorbital region is important. The aim of this study is to analyze blepharoplasty patients according to preoperative deformities and postoperative results and to create a basic algorithm for periorbital aesthetic surgery.

Methods:
Patients who underwent periorbital aesthetic surgery were examined retrospectively. Patients’ medical records and preoperative and postoperative photographs were analyzed. Preoperative and postoperative photographs were analyzed for 6 main criteria: brow position, blepharoptosis, canthal tilt, excess skin or deficiencies, excess subcutaneous tissue or deficiencies, and periorbital skin rhytides. After the anatomical analyses, researchers rated the results as optimal or suboptimal.

Results:
In total, 176 patients’ medical records were analyzed. Among them, 154 were women and 22 were men. The mean age was 43.8 years. No comorbid situation was detected in 133 of these patients. The postoperative result was evaluated as suboptimal in 151 patients (86%) and optimal in 25 patients (14%). An algorithm was proposed for the systematic evaluation of periorbital surgery patients to achieve satisfactory results.

Conclusions:
A simple 6-step checklist is designed to define periorbital deformities and to achieve the optimal youthful appearance.
The Effect of Axillary Lymph Node Sampling during Mastectomy on Immediate Alloplastic Breast Reconstruction Complications
Authors: Verma Richa , Klein Gabriel , Dagum Alexander , Khan Sami , Bui Duc T. 
Published: May 2019

Background:
Tissue expander-based immediate breast reconstruction is currently the most common technique used for postmastectomy breast reconstruction. During mastectomy, axillary lymph nodes are biopsied to stage patients. The purpose of this study is to investigate postoperative complications with respect to extent of lymph node dissection.

Methods:
A retrospective review of all patients undergoing tissue expander-based immediate breast reconstruction at our institution from 2010 to 2012 was conducted. Charts were analyzed to determine the association between the absolute number of axillary lymph nodes removed and postreconstructive incidence of skin necrosis, cellulitis, seroma, and expander removal. Independent sample t test and linear regression were used to analyze data.

Results:
In total, 282 patients with 467 reconstructions were included. Overall incidence of all postoperative complications per breast was 23.8%. Breasts in which a complication occurred had a mean of 6 nodes removed versus 4 nodes in uncomplicated breasts (P = 0.018). Complications were noted at a significantly higher rate in patients who underwent axillary lymph node dissection compared with sentinel lymph node biopsy (P = 0.008). Expander removal and seroma occurred more frequently in breasts that had a greater number of nodes removed (P = 0.006 and P = 0.015, respectively). Preoperative radiation resulted in higher incidence of cellulitis and skin necrosis. Postoperative radiation and chemotherapy did not adversely affect reconstruction.

Conclusions:
Axillary lymph node removal of >4 nodes confers a greater risk of postreconstructive seroma formation and tissue expander loss in patients undergoing immediate reconstruction following mastectomy. Axillary lymph node dissection has a higher incidence of breast reconstruction complications compared with sentinel lymph node biopsy. Therefore, we encourage plastic surgeons to consider degree of lymphadenectomy when discussing reconstructive options with patients, as this may significantly impact their reconstructive outcome.