bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness, safety, and patient‐reported outcomes of emtricitabine/tenofovir alafenamide‐based regimens for the treatment of HIV‐1 infection: Final 24‐month results from the prospective German TAFNES cohort study
Authors: Stephan Christoph , Spinner Christoph D. , Rieke Ansgar , Christensen Stefan , Mauss Stefan , Schreiber Sandra , Albuquerque Boris , Heinzkill Marion , Ramroth Heribert , Stellbrink Hans‐Jürgen 
Published: October 2024

AbstractBackgroundTenofovir alafenamide (TAF) was introduced in the European Union in 2015 as a novel prodrug of tenofovir showing similar efficacy in clinical trials and a more favorable safety profile than tenofovir disoproxil fumarate (TDF). The German TAFNES cohort study (2016–2019) was conducted to generate real‐world evidence.MethodsTreatment‐naïve (TN) and treatment‐experienced (TE) people with HIV (PWH) receiving elvitegravir/cobicistat/emtricitabine/TAF (E/C/F/TAF), rilpivirine/F/TAF (R/F/TAF) or F/TAF + 3rd agent were included. Month (M) 24 outcomes included virologic effectiveness (HIV RNA <50 copies/mL), treatment persistence, adverse drug reactions (ADRs) and patient‐reported outcomes, using the HIV Symptom Index (HIV‐SI), 36‐Item Short Form Health Survey (SF‐36) and HIV Treatment Satisfaction (HIVTSQ) questionnaires.ResultsThe study included 767 PWH (92% men, median age 46 years; 301 TN, 466 TE; E/C/F/TAF [n = 318], R/F/TAF [n = 192], F/TAF + 3rd agent [n = 257]). Among TN, 35% had late HIV diagnosis (CD4 < 350/μL and/or AIDS). Of TE, 95% were on suppressive antiretroviral therapy (ART) before switching. D:A:D (Data Collection on Adverse Effects of Anti‐HIV Drugs) 5‐year risks for chronic kidney disease were high for about 1 in 10 TN and 4 in 10 TE. Overall treatment persistence at M24 was 81% (E/C/F/TAF: 88%; R/F/TAF: 86%; F/TAF + 3rd agent: 70%, with ART simplification of multiple‐tablet regimens in 13%). M24 viral suppression (missing = excluded) was 96% (479/501). Discontinuations due to virologic failure or ADRs were rare, 2% (12/767) and 4% (30/767), respectively. HIV‐SI and SF‐36 summary scores improved in TN; HIVTSQ change scores showed an improvement in treatment satisfaction in TE.ConclusionReal‐world data confirmed a favorable safety profile and high virologic effectiveness with high treatment satisfaction on F/TAF‐based ART.
Effects of 0.2% Hyaluronic Acid Gel‐Impregnated Dental Floss on Clinical Gingival Parameters: A Randomised Clinical Trial
Authors: Atagün Özlem Saraç , Şen Seval Ceylan , Ustaoğlu Gülbahar , Özcan Erkan 
Published: October 2024

ABSTRACTObjectivesGingivitis is a common inflammatory lesion caused by the build‐up of oral biofilm and is an essential precursor to periodontitis. For its treatment, oral hygiene habits, such as dental flossing, must be improved, and adjunctive materials, such as hyaluronic acid, may be used to reduce plaque formation and gingival inflammation. This study aimed to assess the effects of 0.2% hyaluronic acid gel‐impregnated dental floss on the clinical periodontal markers of patients with gingivitis.Material and MethodsThis clinical study adopted a split‐mouth, randomised controlled trial design. After clinical data were assessed at baseline, and supragingival scaling was performed, bilateral gingivitis regions were randomly allocated to either the hyaluronic acid gel‐impregnated floss group or just the floss group using a computer‐generated randomisation table. Clinical parameters were recorded at 1, 2 and 4 weeks after treatment.ResultsOver the 4‐week trial period, all patients showed significant improvements in all clinical periodontal markers. The 1‐week plaque index, 4‐week gingival index and 4‐week papillary bleeding index were significantly lower in the test group than in the control group (p < 0.05).ConclusionsThe use of hyaluronic acid‐impregnated dental floss has resulted in more significant improvements in clinical periodontal parameters compared to dental floss alone. For ease of use, ready‐made products with this innovative formulation may be produced.Trial Registration: ClinicalTrials.gov identifier: NCT06307041
Outcomes of Nonsurgical Periodontal Therapy in Gingivitis and Periodontitis Patients Treated by Senior Dental Students
Authors: Badahdah Arwa , Bahanan Lina , Barnawi Rasha F. , Alghamdi Afrah A. , Banjar Arwa A. 
Published: October 2024

ABSTRACTObjectivesThis retrospective study aimed to evaluate the outcomes of nonsurgical periodontal therapy performed by senior dental students.MethodsElectronic records of patients treated by senior dental students at the comprehensive care clinic in King Abdulaziz University Dental Hospital were reviewed retrospectively. Patients diagnosed with gingivitis or periodontitis who had undergone at least one session of scaling and root planing were included in this study. The following periodontal parameters were assessed: BOP, PI, PD and number of residual pockets.ResultsA total of 618 patients were included in this study (60.2% females and 39.8% males). BOP reduced from a median of 40% (IQR: 26.0%–62.0%) at baseline to 13% (IQR: 9.0%–20.3%) at re‐evaluation in the gingivitis group (p < 0.0005). Similarly, a significant reduction in BOP, from 45% (IQR: 28.0%–64.3%) at baseline to 14% (IQR: 9.0%–21.5%) after phase I therapy, was observed in the periodontitis group (p > 0.001). In both groups, 31%–32% of patients had BOP < 10 at re‐evaluation. PD of ≥ 5 mm was observed in 50.8% of patients with periodontitis at baseline; however, 38% of these patients had no residual pockets at re‐evaluation.ConclusionNSPT performed by senior dental students was effective in improving the periodontal status of the patients. Both gingivitis and periodontitis patients showed significant improvement in BOP and PI. Approximately, 32% of patients showed a reduction in BOP suggestive of a bleeding score associated with periodontal health. Moreover, patients diagnosed with periodontitis exhibited a significant reduction in the number of sites with deep probing (≥ 5).
Postoperative nursing in robot‐assisted urologic surgery: Are there any platform‐based differences?
Authors: Terzoni Stefano , Ferrara Paolo , Maruccia Serena , Pinna Barbara , Parozzi Mauro , Gaia Giorgia , Lusignani Maura , Rocco Bernardo , Sighinolfi Chiara 
Published: October 2024

AbstractMany robotic platforms are currently available for urologic surgery, each with its technical characteristics. It can be hypothesised that these characteristics might affect postoperative nursing, as they affect several aspects of surgery. They could, therefore, impact the surgical outcomes and wound characteristics, involving aspects of postoperative care such as mobilization, nutrition and length of stay. Although data are available on surgical technique aspects and intraoperative nursing, studies have yet to offer insights and considerations about the potential effect of having different robotic platforms on postoperative nursing care. We aimed to verify whether the technical features of the available robots could impact postoperative nursing. We conducted an integrative review on PubMed, CINAHL and Scopus, using Medical Subject Headings (MeSH) and free‐text terms; wildcards and boolean operators were also used. Studies of any design were included. Fifteen papers were retrieved; the key themes emerging from the literature regard trocar setup, technical characteristics of the robots (e.g. single or multiple ports), pneumoperitoneum and Trendelenburg degree. According to the literature, none of these characteristics produce differences in postoperative nursing. The fundamental concepts of postoperative care in surgical inpatients stay the same no matter the robotic platform used. Having competing platforms able to ensure reduced complications without impacting postoperative nursing allows health care facilities to adopt the newest technologies without the need for differentiated nursing protocols. This is an advantage in times of global nursing shortage as it avoids the need for additional platform‐based nursing training.
The prototypical interferonopathy: Aicardi‐Goutières syndrome from bedside to bench
Authors: Hofer Markus J. , Modesti Nicholson , Coufal Nicole G. , Wang Qingde , Sase Sunetra , Miner Jonathan J. , Vanderver Adeline , Bennett Mariko L. 
Published: October 2024

SummaryAicardi‐Goutières syndrome (AGS) is a progressive genetic encephalopathy caused by pathogenic mutations in genes controlling cellular anti‐viral responses and nucleic acid metabolism. The mutations initiate autoinflammatory processes in the brain and systemically that are triggered by chronic overproduction of type I interferon (IFN), including IFN‐alpha. Emerging disease‐directed therapies aim to dampen autoinflammation and block cellular responses to IFN production, creating an urgent and unmet need to understand better which cells, compartments, and mechanisms underlying disease pathogenesis. In this review, we highlight existing pre‐clinical models of AGS and our current understanding of how causative genetic mutations promote disease in AGS, to promote new model development and a continued focus on improving and directing future therapies.
Higher Contribution of Generalists Than Specialists to the Bacterial Biogeography Across Lakes in Northern China
Authors: Hu Yang , Cai Jian , Chao Jianying , Gong Yi , Jiang Xingyu , Tang Xiangming , Shao Keqiang , Gao Guang 
Published: October 2024

ABSTRACTAimGiven that microbial communities normally comprise generalist and specialist species, there have been limited efforts to estimate the contribution of these sub‐communities to bacterial biogeography regarding ecological assembly processes and nestedness structure. To resolve this gap, we studied the spatial distribution of bacterioplankton communities and their generalist and specialist sub‐communities, as well as the underlying assembly processes.LocationSix lakes span three climate zones in northern China.Time PeriodSeptember 2021.MethodsBy separately analysing the generalist and specialist sub‐communities from the whole communities, we investigated the relationships between sub‐communities and the entire communities in terms of distance‐decay pattern, nestedness, and assembly processes. As such, we assessed the contribution of generalist and specialist sub‐communities to the biogeographic patterns of the whole communities.Major Taxa StudiesBacterial community.ResultsGeneralist sub‐communities were more diverse but less abundant than specialist sub‐communities. In line with the whole communities, both types of sub‐communities exhibited distance‐decay relationships at the regional scale, with the slope of specialists being steeper than that of generalists. Additionally, generalist sub‐communities and whole communities also exhibited similar nestedness patterns, in which assemblages from saline water were the subset of those from freshwater. In terms of assembly processes, generalist sub‐communities and whole communities were strongly regulated by homogeneous selection, whereas specialist sub‐communities were mainly shaped by dispersal limitation. More importantly, there was a transition from stochastic to deterministic assembly processes for generalist sub‐communities and whole communities and generalist sub‐communities with increasing total dissolved solids.Main ConclusionsRelative to the specialists, the assembly processes and nestedness pattern were more altered if the generalists were absent, which implies the higher contribution of generalist sub‐communities on the biogeography of whole communities at the regional scale.
Safer and targeted use of antipsychotics in youth: an embedded, pragmatic randomized trial
Authors: Penfold Robert B. , Idu Abisola E. , Coley R. Yates , Cushing‐Haugen Kara L. , King Deborah , Glass Ashley , Phillips Rebecca C. , Renz Anne D. , Pabiniak Chester J. , Graham Vina F. , Thompson Ella E. , Ralston James D. , Simon Gregory E. , Gonzalez Erin S. , Myers Kathleen M. , Beck Arne , Quintana LeeAnn M. , Runkle Arthur J. , Rogers Megan , Foster Deirdre M. , Clarke Gregory N. , Massimino Stefan , Crawford Phillip M. , Cavese Julie A. , Cordaro Anthony R. , Chavez Laura I. , Kelleher Kelly J. , Schwartz Nadine , Jiner Kristina R. , Liu Swan Bee , Condrac Sara , Hilt Robert J. 
Published: October 2024

BackgroundAntipsychotic medications (AP) are inappropriately prescribed to young people. The goal of this pragmatic trial was to test a four‐component approach to improved targeting of antipsychotic prescribing to people aged ≥3 and <18 years.MethodsClinicians in four health systems were cluster randomized by the number of previous AP orders and service line – specialty mental health and all others. Intervention arm clinicians received a best practice alert and child psychiatrist consultation and feedback. Families received system navigation and expedited access to psychotherapy. Primary outcomes were total days' supply of AP medication and proportion of youth with any AP supply at 6 months. We estimated the log‐odds of AP use at 6 months and the relative rate of AP over 6 months. The Safer and Targeted Use of Antipsychotics in Youth (SUAY) trial took place between 3/2018 and 12/2020.ResultsThe trial enrolled 733 patients. The odds ratio (OR) comparing use at 6 months was 0.75 (95% CI: 0.52, 1.09). The mean number of days using AP was 118.5 for intervention patients and 128.2 for control patients (relative risk [RR] = 0.92; 95% CI: 0.81–1.04). Exploratory heterogeneity of treatment effects (HTE) was not detected in groups defined by age, gender, provider specialty, and insurance type. HTE by race/ethnicity was present: among youth of color, mean days' supply was 103.2 for intervention arm and 131.2 for the control arm (RR 0.79, 95% CI: 0.67–0.93). Among secondary outcomes, only new psychotherapy referrals differed with 44.3% (n = 154) of intervention participants having a new order for psychotherapy compared to 33.5% (n = 129) in the control arm (OR 1.47: 95% CI: 1.01–2.14).ConclusionsThis intervention did not result in less AP use at 6 months or a reduction in the days' supply of AP medication, although psychotherapy orders increased. The intervention may be effective for some subgroups.
Impact of COVID‐19 on health‐related quality of life in patients with cardiovascular disease: A cross‐sectional study
Authors: Nurmukanova Dinara , Kurakbayev Kuralbay , Karataev Madamin , Baisugurova Venera , Kashafutdinova Gulzhakhan 
Published: October 2024

AbstractBackgroundThe COVID‐19 pandemic disrupted healthcare access for cardiovascular patients, potentially worsening their health outcomes and quality of life through both medical and psychological impacts.AimsThis cross‐sectional study was conducted to assess the quality of life of patients with cardiovascular disease before and after the COVID‐19 pandemic.Materials and MethodsThe work was conducted from January 9 to April 28, 2023, in Almaty city and the Almaty region. 3026 outpatients with cardiovascular pathology participated in the survey using a standardised European Quality of Life Questionnaire. The study utilized cluster, frequency analysis, and discrete statistics to analyze the impact of COVID‐19 on quality of life parameters, revealing significant changes in anxiety and depression among cardiovascular patients, resulting in reduced quality of life (from 0.3% to 5.5% and 0.4; to 7.5% in the categories of extremely severe and severe manifestations, respectively).ResultsAn analysis of the nosological structure of cardiovascular diseases in the study group was performed and revealed that coronary heart disease occurred in 51.6% of cases, arterial hypertension in 88%, and diabetes mellitus in 34.6%. The availability of both outpatient and inpatient medical services in Almaty region and Almaty city was also assessed, and their comparative analysis was conducted, during which it was found that the need for these services in the district settlements is 10% and 12% higher, respectively, than in the city, which is particularly important for understanding the effectiveness of the public health system in the context of the COVID‐19 pandemic, both at the regional and republican levels.ConclusionThis study is of practical importance for health care workers, as it allows assessing the impact of the COVID‐19 pandemic on the quality of life of patients with cardiovascular diseases, which provides an opportunity to develop effective rehabilitation protocols and psychological support for this vulnerable category.
Alveolar Bone Reconstruction Simultaneous to Implant Removal due to Advanced Peri‐Implantitis Defects: A Proof of Concept
Authors: Monje Alberto , Soldini Maria Costanza , Rosen Paul S. , Tarnow Dennis , Nart Jose , Pons Ramón 
Published: October 2024

ABSTRACTObjectiveTo evaluate the safety and effectiveness of alveolar bone reconstruction simultaneous to implant removal due to peri‐implantitis.Material and MethodsPartial or fully dentulous patients subjected to implant removal due to advanced peri‐implantitis (≥ 50% of bone loss) lesions and seeking to have the failed implant replaced for esthetic or functional reasons were consecutively included. Guided bone regeneration was performed by means of a mixture of xenograft and autogenous bone and a ribose cross‐linked barrier membrane. Re‐entry for implant placement was performed at 4‐month follow‐up. Overall, six radiographic variables were assessed before (T0) and after (T1) alveolar bone reconstruction at four levels in ridge width (RW) and height (RH). Peri‐implant conditions were evaluated at latest follow‐up. Simple and multiple binary logistic regression models were calculated using generalized estimation equations to evaluate the effect of baseline upon reconstructive outcomes.ResultsIn total, 20 patients (nimplant = 39) met the inclusion criteria. Alveolar RW and RH were augmented from T0 to T1 at all levels. All implants achieved primary stability. Only ~13% were subjected to ancillary bone regeneration simultaneous to implant placement. After a mean follow‐up period after loading of ~2.2 years, ~70% implants demonstrated peri‐implant health, while mucositis was diagnosed in the remaining implants.ConclusionThe performance of alveolar bone reconstruction in residual partially contained defects simultaneous to implant removal due to peri‐implantitis lesions demonstrates being safe and effective for implant site development.
The MON1–CCZ1 complex plays dual roles in autophagic degradation and vacuolar protein transport in rice
Authors: Zhang Binglei , Wang Yihua , Zhu Yun , Pan Tian , Yan Haigang , Wang Xin , Jing Ruonan , Wu Hongming , Wang Fan , Zhang Yu , Bao Xiuhao , Wang Yongfei , Zhang Pengcheng , Chen Yu , Duan Erchao , Han Xiaohang , Wan Gexing , Yan Mengyuan , Sun Xiejun , Lei Cailin , Cheng Zhijun , Zhao Zhichao , Jiang Ling , Bao Yiqun , Ren Yulong , Wan Jianmin 
Published: October 2024

ABSTRACTAutophagy is a highly conserved cellular program in eukaryotic cells which mediates the degradation of cytoplasmic components through the lysosome, also named the vacuole in plants. However, the molecular mechanisms underlying the fusion of autophagosomes with the vacuole remain unclear. Here, we report the functional characterization of a rice (Oryza sativa) mutant with defects in storage protein transport in endosperm cells and accumulation of numerous autophagosomes in root cells. Cytological and immunocytochemical experiments showed that this mutant exhibits a defect in the fusion between autophagosomes and vacuoles. The mutant harbors a loss‐of‐function mutation in the rice homolog of Arabidopsis thaliana MONENSIN SENSITIVITY1 (MON1). Biochemical and genetic evidence revealed a synergistic interaction between rice MON1 and AUTOPHAGY‐RELATED 8a in maintaining normal growth and development. In addition, the rice mon1 mutant disrupted storage protein sorting to protein storage vacuoles. Furthermore, quantitative proteomics verified that the loss of MON1 function influenced diverse biological pathways including autophagy and vacuolar transport, thus decreasing the transport of autophagic and vacuolar cargoes to vacuoles. Together, our findings establish a molecular link between autophagy and vacuolar protein transport, and offer insights into the dual functions of the MON1–CCZ1 (CAFFEINE ZINC SENSITIVITY1) complex in plants.
Effect of rooming‐in kangaroo mother care on breastfeeding and behavioral status of full‐term newborns
Authors: Ying Yin , Chen Sichao , Bei Lijun , Ye Junhua , Jin Shan 
Published: October 2024

AbstractObjectivesWe aimed to assess the effects of rooming‐in kangaroo mother care (KMC) on the breastfeeding and behavioral status of full‐term newborns.Materials and methodsFull‐term newborns born from June to December 2021 were divided into Group A (n = 50) and Group B (n = 50) using a random number table. The rooming‐in routine nursing intervention and KMC were given to Group A (n = 50) and Group B, respectively. The outcomes were compared.ResultsThe scores of breastfeeding knowledge, breastfeeding skills, and sense of responsibility for breastfeeding of Group B were higher than those of Group A (p < 0.05). The breastfeeding rate and lactation yield within 3 days of Group B were higher than those of Group A (p < 0.05). Compared with Group A, Group B had higher effective sucking times of newborns and lower behavioral status scores 1, 2, and 3 days after birth, and shorter duration of crying after invasive operation (p < 0.05). The newborns in Group B had better sleep quality, longer sleep time, and lower procedural pain score than those of Group A (p < 0.05).ConclusionRooming‐in KMC improves the maternal breastfeeding self‐efficacy, breastfeeding rate, and lactation yield, ameliorates the behavioral status and sleep quality of newborns, shortens the crying time and relieves the procedural pain.
ZC3H13‐induced the m6A modification of hsa_circ_0081723 promotes cervical cancer progression via AMPK/p53 pathway
Authors: Wei Qiong , Yang Yi , Li Chun , Wang Huimin 
Published: October 2024

AbstractBackgroundN6‐methyladenosine (m6A) modification and circular RNAs (circRNAs) have been confirmed to participate in cervical cancer (CC) progression. However, the function of a novel circRNA, hsa_circ_0081723, has not yet been explored in CC. Therefore, this study aimed to investigate the potential role of hsa_circ_0081723 and its m6A modification in CC.MethodsThe hsa_circ_0081723 and ZC3H13 expressions were examined by qRT‐PCR in the CC tissues, and their prognostic significance was evaluated via Kaplan–Meier Plotter. The role of hsa_circ_0081723 in CC progression was checked by loss‐of‐function assays. The relative protein levels of AMPK/p53 pathway were determined by western blotting. The interactions of hsa_circ_0081723 and ZC3H13 were verified via MeRIP and RNA stability assays.ResultsThe hsa_circ_0081723 expression was elevated in CC samples, and its higher levels indicated high histological grade, high FIGO stage, poor differentiation, and poor prognosis. Functionally, silencing hsa_circ_0081723 impaired the malignant behavior of CC cells and enhanced the protein levels of key molecules of the AMPK signaling pathway. Moreover, ZC3H13 was also elevated in CC samples and demonstrated a positive association with hsa_circ_0081723. The relative enrichment of hsa_circ_0081723 m6A and its stability were enhanced in ZC3H13 overexpressed CC cells. Mechanically, ZC3H13 overexpression partially reversed the antitumor effects caused by hsa_circ_0081723 knockdown in CC cells.ConclusionsThis study innovatively demonstrates that ZC3H13‐mediated m6A modification of hsa_circ_0081723 promotes CC progression by modulating AMPK/p53 pathway. Our findings may contribute to the understanding of the molecular mechanisms underlying CC and offer potential therapeutic targets for clinical treatment.