bluuebunny/crossref_metadata_2025_split huggingface dataset:
Public innovation and policy change in the security policy in Nuevo León
Authors: Bagatella Juan Carlos Montero 
Published: November 2024

AbstractThis article explores the dynamics of policy change and innovation in the context of the security crises in Nuevo León, Mexico, drawing insight from past experiences and ongoing challenges. From 2003 to 2023, with an emphasis on 2006–2011, the article examines the role of the state government and civil participation in shaping innovative policy responses. Key findings highlight the importance of collaboration between government, civil society, and the private sector in developing effective and sustainable solutions. While successful initiatives like the establishment of Fuerza Civil demonstrate the potential of public innovation, challenges such as funding uncertainties and changes in political leadership underscore the need for institutionalized mechanisms for collaboration and evaluation. The article concludes by emphasizing the importance of transparency, accountability, and inclusivity in policymaking processes; and advocating for continued civil engagement to address security challenges and foster a safer and more resilient future.
Microvesicle Tissue Factor Procoagulant Activity Is Elevated and Correlated With Disease Severity in Patients With Cirrhosis
Authors: Protopapas Adonis A. , Takardaki Anna , Protopapa Nefeli , Papagiouvanni Ioanna , Protopapas Andreas N. , Skoura Lemonia , Savopoulos Christos , Goulis Ioannis 
Published: November 2024

ABSTRACTBackground and AimsTissue factor‐expressing microvesicles (MV‐TF) have been found to correlate with thrombotic complications in various diseases. Simultaneously, there is expanding research regarding the effect of the coagulation cascade on liver fibrosis progression. The aim of our manuscript was to evaluate MV‐TF activity in patients with cirrhosis and its correlation with disease severity.MethodsWe prospectively enrolled 82 patients [11 with cirrhosis and hepatocellular cancer (Group 1), 50 with cirrhosis (Group 2) and 21 controls (Group 3)]. Extensive workup for disease staging and exclusion criteria was undertaken. Exclusion criteria included thrombophilia, history of thrombosis, recent hospitalisation, ongoing infection, alcohol dependence, cancer, haematological diseases and use of anticoagulant, antiplatelet or contraceptive drugs. Plasma tissue factor antigen concentration and MV‐TF activity were assessed.ResultsMV‐TF showed median values of 4.03 [1.57], 3.17 [1.59] and 2.26 [1.23] pg/mL in Groups 1, 2 and 3, respectively. There was a statistically significant difference between Groups 1 and 3 (p < 0.001) and Groups 2 and 3 (p = 0.003), while Group 1 had higher values than Group 2 without statistical significance (p = 0.088). In Group 2, the patients' Child‐Pugh (CP) stage was A in 56%, B in 26% and C in 18% of cases. MV‐TF activity significantly correlated with decompensated cirrhosis (p = 0.005) and higher CP stage (p = 0.011). Finally, MV‐TF activity significantly correlated with 12‐month mortality (p = 0.021).ConclusionsMV‐TF activity is elevated in patients with cirrhosis, showing a significant correlation with disease severity. MV‐TF may play a role in the procoagulant imbalance of liver cirrhosis and their contribution in disease progression should be studied further.
Strategizing under conditions of Weberian bureaucracy and ethnic consociationalism
Authors: Ongaro Edoardo , Sancino Alessandro , Mendy Sampierre 
Published: November 2024

AbstractThis study employs a single embedded case study design to investigate strategizing processes under conditions of Weberian bureaucracy and ethnic consociationalism. Drawing from a blend of secondary and primary sources, including semi‐structured interviews, observations, and focus group discussions, we harness thematic and narrative qualitative analysis for generating knowledge about strategizing processes under conditions of Weberian bureaucracy and ethnic consociationalism, as apparent in an African public sector context, through the study of the case of The Gambia Ministry of Basic and Secondary Education. The article contributes to understanding the dynamics of strategizing processes, offering insights that can enhance strategizing practices in similar contexts.
On the Equations of Compressible Fluid Dynamics With Cattaneo‐Type Extensions for the Heat Flux: Symmetrizability and Relaxation Structure
Authors: Angeles Felipe 
Published: November 2024

ABSTRACTThe aim of this work is twofold. From a mathematical point of view, we show the existence of a hyperbolic system of equations that is not symmetrizable in the sense of Friedrichs. Such system appears in the theory of compressible fluid dynamics with Cattaneo‐type extensions for the heat flux. In contrast, the linearizations of such system around constant equilibrium solutions have Friedrichs symmetrizers. Then, from a physical perspective, we aim to understand the relaxation term appearing in this system. By noticing the violation of the Kawashima–Shizuta condition, locally and smoothly, with respect to the Fourier frequencies, we construct persistent waves, that is, solutions preserving the norm for all times that are not dissipated by the relaxation terms.
Machine learning‐enhanced multi‐trait genomic prediction for optimizing cannabinoid profiles in cannabis
Authors: Yoosefzadeh Najafabadi Mohsen , Torkamaneh Davoud 
Published: November 2024

SUMMARYCannabis sativa L., known for its medicinal and psychoactive properties, has recently experienced rapid market expansion but remains understudied in terms of its fundamental biology due to historical prohibitions. This pioneering study implements GS and ML to optimize cannabinoid profiles in cannabis breeding. We analyzed a representative population of drug‐type cannabis accessions, quantifying major cannabinoids and utilizing high‐density genotyping with 250K SNPs for GS. Our evaluations of various models—including ML algorithms, statistical methods, and Bayesian approaches—highlighted Random Forest's superior predictive accuracy for single and multi‐trait genomic predictions, particularly for THC, CBD, and their precursors. Multi‐trait analyses elucidated complex genetic interdependencies and identified key loci crucial to cannabinoid biosynthesis. These results demonstrate the efficacy of integrating GS and ML in developing cannabis varieties with tailored cannabinoid profiles.
Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors
Authors: Zhang Xiujuan , Hao Siyuan , Feng Zehua , Ning Kang , Aksu Kuz Cagla , McFarlin Shane , Richart Donovan , Cheng Fang , Zhang-Chen Ander , McFarlane Richenda , Yan Ziying , Qiu Jianming 
Published: November 2024

ABSTRACT

We conducted a genome-wide CRISPR/Cas9 screen in suspension 293 F cells transduced with rAAV5. The highly selected genes revealed after two rounds of screening included the previously reported
KIAA0319L
,
TM9SF2
, and
RNF121
, along with a cluster of genes involved in glycan biogenesis, Golgi apparatus localization, and endoplasmic reticulum penetration. In this report, we focused on solute carrier family 35 member A1 (
SLC35A1
), a Golgi apparatus-localized cytidine 5’-monophosphate-sialic acid (CMP-SIA) transporter. We confirmed that
SLC35A1
knockout (KO) significantly decreased rAAV5 transduction to a level lower than that observed in
KIAA0319L
or
TM9SF2
KO cells. Although
SLC35A1
KO drastically reduced the expression of α2,6-linked SIA on the cell surface, the expression of α2,3-linked SIA, as well as the cell binding and internalization of rAAV5, was only moderately affected. Moreover,
SLC35A1
KO significantly diminished the transduction of AAV multi-serotypes, including rAAV2 and rAAV3, which do not utilize SIAs for primary attachment. Notably, the SLC35A1 KO markedly increased transduction of rAAV9 and rAAV11, which primarily attach to cells via binding to galactose. Further analyses revealed that
SLC35A1
KO significantly decreased vector nuclear import. More importantly, although the C-terminal cytoplasmic tail deletion (∆C Tail) mutant of SLC35A1 did not drastically decrease SIA expression, it significantly decreased rAAV transduction, as well as vector nuclear import, suggesting that the C-tail is critical in these processes. Furthermore, the T128A mutant significantly decreased SIA expression but still supported rAAV transduction and nuclear import. These findings highlight the involvement of the CMP-SIA transporter in the intracellular trafficking of rAAV vectors post-internalization.

IMPORTANCE
rAAV is an essential tool for gene delivery in the treatment of genetic disorders; however, the mechanisms of rAAV transduction remain partially understood. GPR108 is vital for the transduction of most rAAV vectors, but not for rAAV5. We aimed to identify host factors that impact AAV5 transduction akin to GPR108. Using a genome-wide CRISPR/Cas9 screen in 293 F cells, we identified SLC35A1, a Golgi apparatus-localized CMP-sialic acid transporter that transports CMP-sialic acid from the cytoplasm into the Golgi apparatus for sialylation, is essential to rAAV transduction. Further studies across various AAV serotypes showed SLC35A1 significantly affects vector nuclear import post-internalization. These results underscore the crucial role of SLC35A1 in intracellular trafficking beyond the initial cell attachment of rAAV.
Complete genomes of
Mucilaginibacter sabulilitoris
SNA2 and
Mucilaginibacter
sp. cycad4: microbes with the potential for plant growth promotion
Authors: Hirsch Ann M. , Humm Ethan , Rubbi Liudmilla , Del Vecchio Giorgia , Ha Sung Min , Pellegrini Matteo , Gunsalus Robert P. 
Published: November 2024

ABSTRACT

Mucilaginibacter
species have been isolated from various environments, often in association with plants. Here, we report the complete genomes of
Mucilaginibacter sabulilitoris
SNA2 and
Mucilaginibacter
sp. cycad4. The former is the first available for that species, and based on 16S sequence analysis, the latter strain is likely a new species.
Improving the maternity experience for Black, African, Caribbean and mixed-Black families in an integrated care system: a multigroup community and interprofessional co-production prioritisation exercise using nominal group technique
Authors: Aryasinghe Sarindi , Averill Phoebe , Waithe Carole , Ibuanokpe Susan , Newby-Mayers Rhianna , Lakhdar Nawal , Amine Sylla Moussa , Cox Elizabeth , Das Sabrina , Mayer Erik 
Published: November 2024

BackgroundEthnic inequities in maternity care persist in England for Black, African, Caribbean and mixed-Black heritage families, resulting in poorer care experiences and health outcomes than other minoritised ethnic groups. Co-production using an integrated care approach is crucial for reducing these disparities and improving care quality and safety. Therefore, this study aimed to understand the alignment of health and local authority professional perspectives with community needs on how to improve maternity experiences for this ethnic group within a London integrated care system (ICS).MethodsBetween March and June 2024, five workshops were conducted with health professionals, local authorities, voluntary, community and social enterprise (VCSE) sector and the public from Black, African, Caribbean and mixed-Black heritage backgrounds across the North West London ICS. Using the nominal group technique (NGT), attendees prioritised ideas to improve the experience of maternity care for families from Black, African, Caribbean and mixed-Black heritage backgrounds, which were thematically synthesised using framework analysis.ResultsFifty-four attendees, covering primary, secondary, regional and national health professionals, public health teams from three local authorities, VCSE sector and the public, generated 89 potential interventions across 11 themes. All attendees prioritised improving staff knowledge and capacity in culturally competent care and communication. Community-identified needs for advocacy mechanisms and mental health support throughout the maternity pathway were not reflected in professional priorities.ConclusionThe study highlights the need for an integrated, community-centred approach beyond hospital settings when addressing ethnic inequities in maternity care, recognising key differences between community and professional priorities within an ICS. Leveraging lived experience expertise to lead the NGT community workshops was essential in building trust and buy-in of the overall prioritisation process.
Development and Characterization of a Lysosome-Targeting SLC3A2/PD-L1 Bispecific Antibody–Drug Conjugate for Enhanced Antitumor Efficacy in Solid Tumors
Authors: Wang Zeng , Zheng Meijun , Li Mengyao , Lu Huaqing , Liu Nanxi , Chen Yongdong , Yang Nian , Zeng Wanqin , Dong Yijun , Li Jia , Zhu Zhixiong , Yang Chen , Zhang Zongliang , Lu Qizhong , Li Hexian , Zhou Liangxue , Yang Hui , Tong Aiping 
Published: November 2024

Abstract
Bispecific antibodies (BsAb) and antibody-drug conjugates (ADC) have shown significant promise in cancer treatment, enhancing drug selectivity and therapeutic efficacy as demonstrated in multiple clinical studies. Bispecific antibody-drug conjugates (BsADC), which combine the targeting capabilities of BsAbs with the cytotoxic potential of ADCs, offer a novel approach to overcoming several challenges associated with ADCs, including limited internalization, off-target toxicity, and drug resistance. In this study, we identified solute carrier family 3 member 2 (SLC3A2) as a highly expressed protein in a variety of solid tumors, making it a promising therapeutic target. We developed a BsAb targeting SLC3A2 and PD-L1 and conjugated it to monomethyl auristatin E (MMAE) to create the SLC3A2/PD-L1 BsADC. The SLC3A2/PD-L1 BsAb effectively blocked PD-1 binding to PD-L1 and activated T cells while also facilitating lysosomal targeting and degradation of poorly internalized PD-L1 antibodies. The SLC3A2/PD-L1 BsADC demonstrated superior antitumor efficacy in PD-L1 low-expressing tumor cells compared with single-target ADCs in both in vitro studies and in multiple xenograft and immunocompetent mouse models. Overall, our engineered SLC3A2/PD-L1 BsADC exhibited enhanced internalization and improved tumor cell targeting, highlighting the potential of lysosome-targeting BsAbs in advancing ADC therapeutic strategies for solid tumors.
Lightning and Radar Measures of Mixed-Phase Updraft Variability in Tracked Storms during the TRACER Field Campaign in Houston, Texas
Authors: Bruning Eric C. , Brunner Kelcy N. , van Lier-Walqui Marcus , Logan Timothy , Matsui Toshi 
Published: November 2024

Abstract
Properties of 7488 thunderstorms are summarized for June–September 2022 during the Tracking Aerosol Convection Interactions Experiment (TRACER) field campaign Houston, Texas, using polarimetric weather radar and VHF 3D Lightning Mapping Array data. Automated tracking of storms linked each instrument’s measurements to a data-defined, time-evolving storm footprint. Within each storm, the depth and magnitude of episodic columns of radar differential reflectivity and specific differential phase quantified the prevalence of updrafts that activated mixed-phase precipitation pathways. Lightning measurements further distinguished the degree of rimed precipitation formation: the fraction of tracks with lightning varied from day to day and cells with lightning had stronger polarimetric columns. Track-level correlation of the lightning flash rate with radar polarimetric measures had substantial spread, showing that lightning provides an additional signal of mixed-phase precipitation processes that can complement future studies of thermodynamic and aerosol controls on cloud microphysics in the Houston region.
Evaluation of Door-to-Needle Times Between Alteplase and Tenecteplase for Acute Ischemic Stroke at Two Academic Medical Centers
Authors: Monkemeyer Nicholas J. , Marino Kaylee K. , Goodberlet Melanie Z. , Anderson Christopher , Bresette Linda , Webb Andrew J. , Singhal Aneesh , Ware Lydia R. 
Published: November 2024

Background: Off-label tenecteplase use for acute ischemic stroke (AIS) has increased due to ease of administration and comparable efficacy and safety to alteplase. Objective: This study aimed to evaluate time to thrombolysis before and after transition from alteplase to tenecteplase for treatment of AIS at 2 institutions. Methods: This retrospective cohort study included adult patients receiving thrombolysis for AIS before and after transition from alteplase to tenecteplase at 2 academic medical centers from January 1, 2020 to January 31, 2024. The primary endpoint was door-to-needle (DTN) time, defined as minutes from hospital arrival to thrombolysis administration. Notable secondary endpoints included time from last known well (LKW) to thrombolysis, time from brain imaging to thrombolysis, hospital length of stay (LOS), and incidence of symptomatic intracranial hemorrhage (sICH). Results: A total of 328 patients (168 tenecteplase and 160 alteplase) were included. Patients were 51.5% female with a median (interquartile range [IQR]) age of 70 [58-80] years and initial National Institutes of Health Stroke Scale (NIHSS) score of 8 [5-14]. There was no statistically significant difference in DTN time (60 vs 56 minutes), time from LKW to thrombolysis (134 vs 147.5 minutes), or time from brain imaging to thrombolysis (32 vs 31 minutes) between tenecteplase and alteplase. Hospital LOS (5.7 vs 4.9 days) and the rates of sICH (3% vs 3.8%) were similar between groups. Conclusion and Relevance: Tenecteplase and alteplase had comparable DTN times for treatment of AIS and similar safety endpoints. Further studies are warranted to identify opportunities to streamline DTN times with tenecteplase.
Insights Into the Epidemiology of Peripheral Nerve Injuries in the United States: Systematic Review
Authors: Randall Zachary D. , Navarro Brendan J. , Brogan David M. , Dy Christopher J. 
Published: November 2024

Background: Peripheral nerve injuries (PNI) range from mild neurapraxia to severe transection, leading to significant morbidity. Despite their impact, the societal implications of PNI in the United States are not well understood. This study aims to systematically review the literature on PNI epidemiology in the United States. We hypothesize that this review will reveal significant gaps in the understanding of PNI incidence, demographics, and economic impact. Methods: Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, we queried the literature for studies on PNI that reported at least one of the following: incidence rates, demographics, affected nerve distribution, injury mechanisms, surgical intervention rates, and associated direct costs. Exclusion criteria included non-English publications, abstracts, conference proceedings, reviews, or editorials, studies published before 2000, non-US studies, or studies focusing solely on digital nerves or plexus injuries. Results: Fifteen studies met the inclusion criteria. Data indicate a higher incidence of upper extremity nerve injuries compared with lower extremity injuries. The literature lacks comprehensive reporting on surgical intervention rates, with no recent data since 2013. There is a notable absence of nationwide epidemiological data on PNI mechanisms and recent cost data, with most information over a decade old and primarily focused on inpatient costs, neglecting outpatient visits, physical therapy, and medication expenses. Conclusion: The epidemiological data on PNI are limited and outdated, highlighting the need for further research. Future studies should focus on recent trends in PNI incidence, injury mechanisms, and financial burden, including comprehensive reporting on surgical interventions, to inform strategies aimed at improving patient outcomes and health care resource allocation.
The availability of common patient-reported outcome measures in hand surgery across Europe
Authors: Aman Martin , Pennekamp Anna , Prahm Cosima , Czarnecki Piotr , Van der Heijden Brigitte , Harhaus Leila 
Published: November 2024

Many European healthcare systems emphasize value-based care to improve outcomes and control costs. Assessing function and satisfaction in hand surgery relies on patient reported outcome measures. This study evaluates the use of these outcome measures across Europe, focusing on diversity and language validation. A comprehensive database search for hand-specific patient reported outcome measures assessed availability for all official languages in Europe focusing on transculturally adapted and validated versions of available questionnaires. The study identified 47 European countries with 41 unique languages. The Disabilities of the Arm, Shoulder and Hand Questionnaire is the most available outcome measure, translated into 41 languages. Availability of other outcome measures varied significantly by language. Gaps in the availability of hand specific patient reported outcome measures exist across Europe. To assist in translating and validating such questionnaires, an exemplary Standard Protocol Items: Recommendations for Interventional Trials checklist is provided as supplementary material. Level of evidence: IV
Transulnar approach as an alternative access site for neuroendovascular procedures
Authors: Gómez-Pena Sara , Trejo Carmen , Pérez-García Carlos , López-Frías Alfonso , Rosati Santiago , Schmolling Ángela H , Moreu Manuel 
Published: November 2024

Objective This study aims to describe our experience and safety considerations associated with the use of the transulnar approach in neurointerventional procedures when radial access is not feasible. Methods A prospective exploratory analysis was conducted on 35 patients who underwent diagnostic or therapeutic neuroangiography via transulnar approach due to radial access limitations. Technical aspects of the transulnar approach, patient demographics, procedural details, and complications were documented. Statistical analysis was performed using SPSS Statistics software. Results Among the 35 procedures, the transulnar approach demonstrated a high success rate (97.14%), with only one procedural failure. Indications for transulnar access included larger ulnar artery caliber (46%), radial artery spasm (37%), severe tortuosity of the radial artery (17%), and other anatomical considerations. Complications were rare, with only two access-related complications observed: a type I hematoma and ulnar artery vasospasm. No major bleeding episodes occurred, highlighting the safety profile of transulnar access. Conclusion The transulnar approach represents a feasible alternative for neurointerventional procedures when radial access is not achievable. It offers high success rates and minimal complications, comparable to the radial approach. Anatomical considerations, safety profile, and patient preference support its consideration as a secondary option in neurointerventional practice. Further research and larger studies are warranted to validate its efficacy and safety in a broader patient population.