bluuebunny/crossref_metadata_2025_split huggingface dataset:
ALPHA–LIPOIC ACID ANTIOXIDANT EFFICIENCY IN TREATMENT OF PERITONEAL DIALYSIS PATIENTS
Authors: Korol L. , Stepanova N. , Ablogina O. , Migal L. 
Published: June 2018

The aim of the study was to evaluate the effectiveness of the antioxidant alpha–lipoic acid in peritoneal dialysis (PD) patients taking into account the state of lipid peroxidation (LPO) and antioxidant protection (AOP) before and after treatment.
Patients and methods. The research revealed the intensity of oxidative processes in 20 patients treated with continuous ambulatory peritoneal dialysis (CAPD) on the basis of а–lipoic acid applied parenterally in a dose of 600 mg for 2–weeks followed by a transfer to the 6 weeks of oral reception.
The intensity of oxidative processes was evaluated before and after treatment through determining the content of MDA in serum (MDAs) and erythrocytes (MDAe). AOP activity was evaluated by the content of the antioxidant enzyme ceruloplasmin (CP), transferrin (Tr), SH–groups and total peroxidase activity (TPA) of red blood cells. The control group consisted of30 individuals comparable on for age and sex.
Results. The effects on the study of PD patients (positive decrease of MDAs, increase in CP higher than control group, a significant increase in the content of SH–groups (p=0,04), TPA (p=0,009), Tr (p=0,002) and catalase serum) demonstrated a considerable positive dynamics of LPO /AOP influenced by the use of а–lipoic acid.
Conclusions. The research opens up the prospect for further study ofLPO /AOP blood in PD patients and proves the
application expediency of alpha–lipoic acid for this group. Use of the latter lowers the activity of oxidative processes significantly and restores AOP indicators substantially, which in turn minimizes the negative influence of oxidative stress on a patient’s body.
HEPATITIS IN PATIENTS TREATED BYHEMODIALYSIS
Authors: Palamar B. 
Published: June 2018

During the last decades, according the international registers (in 2011–2013) there is a steady increase of amount of patients with terminal kidney insufficiency. For the aim of continuation a life to such patients we apply the methods of renal replacement therapy, one of each is hemodialysis.
The risk of infecting ofparenterally hepatitis viruses grows in connection with the features ofprocedure of hemodialysis, first of all hepatitis B and С. Persisting in an organism, these viruses can become reason of development of chronic disease of liver, with development of cirrhosis or \and hepatocellular carcinoma.
World experience shows that frequency infecting hepatitis In (HBV) and С (HCV) in terms by treatment of hemodialysis (HD), high and depends, first of all, from intensity of epidemic process in a region, and also from the level of socio–economic development of country.
In this work we analysed prevalence of infecting hepatitis B and С in the different countries of the world, and also outlined basic recommendations in relation on timely diagnostics and warning of infection of hepatitis of patients who treated by hemodialysis.
OXIDATIVE STRESS AND RESISTANCE OF ERYTHROCYTES MEMBRANES IN PATIENTS WITH CHRONIC KIDNEY DISEASE STAGE VD DEPENDING ON MODALITY OF RENAL REPLACEMENT THERAPY
Authors: Korol L. , Mygal L. , Burdeyna O. , Kolesnyk M. 
Published: June 2018

The aim of the research was to study the effect ofoxidative factors impact and modality of renal replacement therapy (BBT) on indices ofoxidative stress (OS) and resistance of erythrocytes membranes in patients with chronic kidney disease stage V(CKD VD) and anemie.
Material and methods. The study involved 68 patients with CKD VD: 14 patients were treated by hemodiafiltration (HDF), 25 patients by hemodialysis (HD) and 29 patients by peritoneal dialysis (PD). The severity ofanemia was assessed according to the KDIGO (2012) criteria. The control group consisted of 30 healthy people of the same age and sex. Along with the standard diagnostic methods, we defined the content of malonic dialdehyde in serum (MDAs) and in erythrocytes (MDAe), the content of ceruloplasmin (CPs), transferrin (TBs) and SH-groups in the blood serum, the index of the OS (IOS), catalase activity in serum (CATs), glucose-6-phosphate dehydrogenase (G-6-PDHe) and total peroxidase activity (TPA) in erythrocyte, peroxide resistance (PR) of red blood cells and erythrocyte membrane permeability (EMP). Statistical analysis was performed using the programs of Microsoft Excel 7.0.
Results. It has been stated that in the CKD VD patients in compatison with control group the MDAs content increased by 3.3 times and MDAe - 1.2 times, TBs content reduced by 34%, SH-groups - by 31%, TPAe - by 41% and G- 6-FDGe - by 58%, PB-by 60%; 4.6 times increased CATs activity and OSI; 2 times grew peroxide hemolysis (PH) and 1.3 times - EMP. The analysis (depending on the BBT modality) showed that the patients treated by HDF had typical MDAs increase by 3.9 times on a background of CPs by 24%, TBs - 33%, SH-groups - 25%, TPAe - 51%, G-6-PDHe - 42%; the increase in serum OSI - 5.4 times and 2.6 times in erythrocytes, PB - by 3.6 times and CATs activity by 3.5 times; HD group was characterized by the highest value of MDAe, OSI, PH and CATs, along with more expressed decrease of TBs indices, SH-groups, TPA and G-6-FDHe activity compared with rates in patients with HDF. The patients treated with PD had the lowest content of MDAs and the highest values on the background ofTPAe, the significant increase of CPs by 1.7 times and lowest TBs and G-6-PDHe. The patients with PD showed twice lower OS activity by OSI.
Conclusion. Thus, in patients with CKD VD, who had HD, HDF or PD an anemie was associated with high OS activity and the increased degree of hemolysis. These changes are stipulated by BBT methods: for patients receiving HDF were typical the lowest rates of hemolysis and the highest degree ofprotection for erythrocytes, and for patients treated with HD - the highest OS.
ASSOCIATION OF HLA AND CYTOKINES OF BLOOD WITH ANTIINFLAMMATORY EFFECTS IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS
Authors: Kolesnyk M. , Driyanska V. , Drannik G. , Petrina O. , Velychko M. , Nepomnyaschiy V. 
Published: June 2018

Cytokines and HLA take important part in immunogenesis of many diseases, therefore the analysis of these indices and this associations in dependence of glomerulonephritis (GN) can define their value as the additional prognostic markers.
Aim of the work is to determine the of associations the high serum levels of cytokines (IL-4, IL-17) and peculiarities of some HLA in phenotype to substantiate of chronic glomerulonephritis with nephrotic syndrome (CGN, NS) immunogen- esis and to ascertain the additional prognostic markers.
Materials and methods. There was studied the HLA-antigens distribution in the 264 adult patients CGN, NS (the diagnosis was confirmed morphologically using the thin needle nephrobiopsy) and 350 healthy donors by typing the lymphocytes with the aid of standard microlymphocytotoxic test (Terasaki’s test). HLA antigens were defined using a standard microlymphocytotoxic test on the Terasaki's planchette with special panels ofanti-HLA serums (20 antigens of locus A, 31 - B and 9 - DR). The etiologic fraction (attributive risks ) was counted using the formula: ct = x - y/I - y, where x - frequency of antigen in patients and y - frequency in healthy. The ct reading was considered significant when it exceeded 0,1. Using ELISA, the level of the cytokines was studied in the blood serum - IL-4 in 76 and IL-17 – 79 patients.
Results. HLA-A23, -24, -28, -B8, -38, -41, -44, DR1, -4, -w52 in adults patients have associations (RR>2) CGN, NS; the attributive risk (AR) (<j>0,1) to develop GN detected in patients have A24, A28, B8, DR 1, 4, w52.
The CGN, NS patients has statistically higher serum level of the IL-4 and IL-17, with more high indices of this cytokines in patients with attributive risk antigens HLA-A24 and A-28. The highest levels of IL-17 detected also in adults case have B14 and B38, which associated with steroid sensitive NS. HLA-B8, which associated with steroid resistant NS, have more patients with CGN, NS with lower serum level of antiinflammatory IL-4.
Conclusion. The patients with CGN, NS have associations of HLA and serum levels of pro- and antiinflammatory cytokines IL-4 and IL-17, which play role of additional prognostic predictors.
BACTERIAL SUSCEPTIBILITY-RESISTANCE LEVELS OF CHRONIC PYELONEPHRITIS PATHOGENS’, DEPENDING ON EXPRESSION OF DIFFERENT TYPES OF PLASMID-MEDIATED RESISTANCE GENES
Authors: Chub О.І. , Bilchenko O.V. , Teslenko S.V. 
Published: June 2018

The aim of the study is to determine the bacterial susceptibility-resistance levels of uropathogens depending on expression of different types of plasmid-mediated resistance genes.
Methods. A cross-sectional study of105 patients with chronic pyelonephritis was carried. Screening for the presence of plasmid-mediated genes was performed by polymerase chain reaction. The antimicrobial susceptibility of isolates was determined by the Kirby Bauer disk diffusion method on Mueller–Hinton agar-containing plates. The size of zone around each antimicrobial disk was interpreted as sensitive, intermediate or resistant.
Results. We demonstrated in vitro significant relationship of the resistance to aminopenicillins, cephalosporins and fluoroquinolones with an appearance of plasmid-mediated extended spectrum p-lactamases (ESBLs) types blaCTX-M, blaTEM, blaSHV and fluoroquinolones resistant genes (PMQR), including QnrA, AAC (6') - Ib-cr, QepA) in bacterial uropathogens. We also demonstrated in vitro significant relationship of the resistance to aminoglycosides with an appearance of plasmid-mediated genes blaCTX-M, QnrA and QepA.
Conclusion. Were established in vitro significant relationships of the resistance to antimicrobials with an appearance of plasmid-mediated resistance genes in uropathogens. Were determined the most effective antibiotics against ESBL and PMQR producers. We have established the algorithm of diagnostic of plasmid-mediated resistance genes in uropathogens, isolated from patients with chronic pyelonephritis.
PECULIARITIES OF CYTOKINES AND THEIR PROGNOSTIC VALUE IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS
Authors: Kolesnyk M. , Driyanska V. , Velichko M. , Drannik G. , Savchenko V. 
Published: June 2018

Introduction. Cytokines are important to take part in immunogenesis and progression of glomerulonephritis (GN), therefore the analysis of these indices in dependence of type of GN forms as prognosys results of treatment.
Aim of the work is to determine the peculiarities of pro-inflammatory cytokines (TNFα, MCP-1, IL-17, -18, -23) and anti-inflammatory TGF- β1 and their association with form GN with nephritic syndrome (NS) and possibility use cytokines as prognostic markers.
Materials and methods. Using IFA, the level of the above mentioned cytokines was studied in the blood serum of 117 patients with proliferative (33) and non-proliferative (84) CGN, NS forms. There was also analyzed the changes of these indices in the patients with subsequent clinical laboratory remission (59) and patients without one (58).
Results. The GN, NS patients showed statistically higher level of the pro- inflammatory cytokines – TNFα, MCP-1, IL-17 (with more high indices last one in the proliferative forms of GN, and IL-17 in nonsensitive to immunotherapy patients). High level profibrogenic TGFβ, and the ratio TNFα /TGFβ reflect the prevalence of anti-inflammatory reactions and high activity of monocytes - macrophages and T-helpers17. The positive effect of the treatment associates with the statistically decreased TNFα, MCP-1 and TGFβ1 levels in the patients with proliferative forms GN; the TGFβ1 - for patients with non-proliferative GN forms.
Conclusion. The changes cytokine profiles may be use as predictors of results the immunosuppressive therapy.
MODERNETIOLOGICAND GENDER FEATURESOFMICROBAL SPECTRUMOFURINE IN TEENAGERS WITH URINARYTRACTINFECTIONS
Authors: Bezruk V.V. , Bezruk T.O. , Blinder O.V. 
Published: June 2018

In Ukraine the increase of amount of teenagers registers with the infection of the urinary system.
Materials and methods. A bacteriological investigation of urine of324 teenagers is undertaken in the Chernivtsi region. Family and specific belonging of the distinguished stamms in the standards of urine was conducted by the generally accepted microbiological methods.
Results. The dynamic increase ofpercent of selection is educed etiologfc and clinically meaningful stamms among the teenagers of both sexes. Selection ofpercent etiologfc and clinically meaningful stamms among girls–teenagers (3,75% – 18,52%) prevails by comparison to their coevals (1,02% – 12,50%). A negative dynamics is marked in megascopic percent of results of bacteriological investigation of urine (1,46% – 14,81%) in a group «Height it is not educed».
Conclusions. Infections of the urinary system children have the issue of the day of nephrology, and needs complex going near the study of the age–related and gender factors of prevalence of Infections of the urinary system among child’s population of the different age–related groups.
THE LEVEL OF MENTAL STRESS, AND SELF - ESTEEM OFCHILDREN’S HEALTH WITH CHRONIC KIDNEY DISEASE
Authors: Dryl I.S. , Shipko A.F. , Kolfbaeva T.F. , Petrenko L.K. , Zabashta I.V. 
Published: June 2018

The most frequent mental changes in children can include increased anxiety, depression, aggression and anger, the existence of which may contribute to the progression of physical illness.
Materials and methods. The study involved 49 children aged 7 to 17 years (mean age 12,7 ± 3,1let) with chronic kidney disease. Further assess psychological stress based on the level of anxiety, aggression, anger, and depression.
Results. In assessing the level of anxiety, aggression, anger, and depression in all groups of children prevailed with the average of 73,1 ± 7% to 87,8 ± 5,1% of children in the group. At 24,3 ± 7% of children identified elevated levels of aggression, half of whom had congenital abnormalities of the urinary system. Most children 38 (86,3 ± 5,2%) level of depression was within average. Established cases of depression to improve small, moderate and significant among girls 13,5 ± 1,5years, which amounted to 13,6 ± 5,2% of children.
Conclusions. The most vulnerable are children, particularly girls with chronic kidney disease on the background of congenital anomalies of the urinary system. All children with chronic kidney disease is necessary to evaluate the level of mental strain for the optimization of measures to prevent recurrence of the disease and improve the quality of life ofchildren.
RENAL RISCS IN DETERMINATION OF BRONCHIAL ASTHMA PHENOTYPES
Authors: Semydotska Zh.D. , Cherniacova I.A. , Eremenko G.V. 
Published: June 2018

The aim of this investigation was to study acid - secretory renal function and acid - base balance in patients with bronchial asthma in order to optimize the diagnostics and treatment from the pposition of phenotyping.
Materials and methods. In 102 patients with moderate bronchial asthma there was studied the excretion of titratable acids by titration method, excretion ofammonium by Van Slyk - Palmer’s method and acid - base status of the organism in the venous blood by blood gas analyzer 348. Respiratory function was studied on computer spirograph «Microlab 330».
Results. Studied patients were divided into 3 groups according to the disorders of acid - base balance and acidsecretory renal function: 62 patients had respiratory acidosis and a significant increase in the excretion of titratable acid and ammonium; in 28 patients metabolic acidosis and a moderate increase in acid - secretory renal function were ascertained; in 12 patients respiratory alkalosis was found, acid - secretory renal function did not differ significantly from that of healthy persons. The most pronounced bronchial obstruction and bronchial hyperreactivity were observed in patients with respiratory alkalosis. The intravenous infusion of buffered 4.2% solution of sodium hydrocarbonate was included in complex therapy in 14patients with metabolic acidosis. After this treatment marked improvement of bronchial obstruction was established, as well as normalization of BE, pCO,, pO,, increased blood pH, improve control over asthma. The possibility to separate asthma phenotypes (subphenotypes) according to the disorders of the acid - base balance and acid - secretory renal function is discussed.
Conclusions. Monitoring of acid - base status and acid - secretory renal function can be used in patients with asthma to separate phenotypes (subphenotypes) for disease diagnostics and treatment optimization.
CLINICAL AND MORPHOLOGICAL COMPARISON AT NEPHROPATHY IN PATIENTS WITH MICROSCOPIC POLYANGIITIS
Authors: Syniachenko O.V. , Bevzenko T.B. , Diadyk O.O. , Iermolaieva M.V. , Iegudina E.D. , Pomazan D.V. 
Published: June 2018

Introduction. Morphological signs of renal pathology in microscopic polyangiitis, their connection with extrarenal manifestations of the disease and urine laboratory parameters, as well as the pathogenesis of such nephropathy remain insufficiently studied, what became the purpose and objectives of this study.
Material and methods. Nephrobiopsy was made 12 patients. The study was performed on microscope «OlumpusBX40», «Olumpus - C3030 - ADU», «Olympus DP - Soft», «Olympus - AX70», «Olympus - DP50».
Results and discussion. Damage of glomeruli, stroma, tubules and vessels was detected without exception in all patients with deposition in the kidney structures of immunoglobulins A, G, M, C3and Clqcomponents of complement that depend on the presence ofcardiac, pulmonary, hepatic, articular and neurological pathologies, and morphological indicators of renal lesion affect the parameters of proteinuria, erythrocythuria, uricosuria, nitrituria and surface physicochemical properties of urine, associated with blood levels ofdifferent antibodies, rheumatoid factor and circulating of immune complexes.
Conclusions. Renal involvement at microscopic polyangiitis affects all morphological structures and indicates the immunecomplex damage of glomeruli, stroma, tubules and vessels.
CLINICAL AND ECONOMIC ANALYSIS OF USING OF LONG ACTION ERYTHROPOES STIMULATED AGENTS FOR THE TREATMENT OF ANEMIA CKD V HD PATIENTS
Authors: Kolesnyk M. , Bezdetko N. , Novakivskyy V. 
Published: June 2018

The article describes the ways to cost optimization of anemia treatment CKD - HD patients.
Aims. dinical and economic analysis treatment of Anemia CKD VHD PTS with using long action Erythropoiesis Stimulating Agents (ESA).
Methods. We calculated the true cost of anemia therapy with darbepoetin alfa compared to the methoxypolyethyleneglycol - epoetin beta.
Results. It was established that average cost of therapy with methoxypolyethyleneglycol - epoetin beta was lower than darbepoetin alfa provided declared, wholesale or retail prices of25 - 60%.
Conclusions. The methoxypolyethyleneglycol - epoetin beta haspharmacoeconomics advantages in comparison with the darbepoetin alfa in treatment of anemia CKD V HD patients.
VASCULAR ACCESS OPTIONS AT HEMODIALYSIS THERAPY INITIATION: COMPLICATIONS AND SURVIVAL OF PATIENTS WITH CHRONIC KIDNEY DISEAS STAGE 5 D
Authors: Shifris І.М. , Dudar I.О. , Gonchar I.І. , Krasyuk E.K. , Prusskiy F. О. , Vburhynska I. 
Published: June 2018

The annual increasing of dialysis population results tofocus world attention on the problems associated with improving of survival among patients with chronic kidney disease stage VD (CKD VD st.)., decreasing iatrogenic complicationfrequency. HD is the most common method ofrenal replacement therapy (RRT). Several studies haveproven the link between the type ofinitial vascular access, and an morbidity and survival of patients with CKD VD st..
Aim. The aim of this study was to explore the effects of an initial vascular access on morbidity and survival inpatients with CKD VD st., which are treated with hemodialysis.
Materials and methods. In the study 79HDpatients were included. Patients were randomized into twogroups, depending on the type of vascular access at the start of HD treatment: the first group (n = 47) included patients, who started HD treatment through theformed AVF, patients among which central venous catheter (CVC) was usedfor the HD initiation composed the second (n= 32) group.
The endpoints were: the total number of deaths, hospitalizations due to bacterial infections, sepsis, unstable angina and myocardial infarction. All cases that took place from 01.01.2013 to 01.12.2015 year were analyzed. Evaluation of survival wasperformed using the Kaplan-Meier method.
Results. The analysis allowed to state that during analyzed period in total were 24 deaths: in the first group ofpatients - 10 (21.28%), and in the second - 14 (43.75%) cases; p = 0.03299. The survival rate ofpatients was significantly lower with the CVC. The 10-years cumulative proportion surviving was 73% and 40% when using of AMT and CVC, respectively. The relative risks and odds ratio for all-cause hospitalization in the second group ofpatients (26 episodes) were significantly higher compared to thefirstgroup (16 cases): RR = 2,387, (95% Cl: 1,550-3,674) and OR = 8,396 (95% Cl: 2,870-24,559). It was established a significant increasing of the hospitalization incidences both due infectious comorbidity (9/21,43% versus 15/46,9%;p = 0,0085), and cardiovascular diseases (7/16.7% vs. 11/34,37%;p = 0,0427) inpatients with a history ofthe CVC use at the HD initiation, compared withpatients who started treatment through theformed AVF.
Conclusion. Thus, the obtainedfindings suggest that the use of the CVC at the HD initiation has a significant negative effect onprognosis in thepatientpopulation with CKD stage VD.
THE FACTORS INFLUENCING RENAL RECOVERY IN CARDIAC SURGERY ASSOCIATED ACUTE KIDNEY INJURY
Authors: Zakon K. , Romanova V. , Tverdokhlib K. , Dudarenko V. , Arbuzova I. , Radchenko G. 
Published: June 2018

Background. In-hospital mortality of cardiac surgery patients with AKI is 3-7 times higher than those without AKI. This prospective observational study was dedicated to evaluate a differential approach of applying continuous and intermittent modalities in CS-AKIpatients on the rate and grade of renalfunction recovery.
Methods. One hundred and six adult cardiac surgery patients admitted hospital in 2008-2011 years, who had AKI and met inclusion criteria were allocated in CRRTorIRRTgroup.
Results. Sixty eight cardiac surgery with AKI patients who needed RRT were discharged from hospital. Among them, recovery of renalfunction was observed in 54 patients (79,4%) and 14 patients (20,6%) remained RRT-depen- dant. Complete recovery of renalfunction was reached in 33 patients (48,5%), while partial was observed in 21 patients (30,9%). Univariate analysis showed that complete recovery of renalfunction in CS-AKI significantly associated with reducing of dopamine dose during thefirst day of RRT (p=0,01) and long-term use of dobutamine (p=0,009). Partial recovery was associated with early dobutamine withdrawal (p=0,005) and absence of MOT (p=0,016), while RRT- dependence at discharge was associated with absence of MOT (p=0,006) and escalation of dopamine dose on thefirst day of RRT (p=0,025). The rate of renal recovery was statistically significantly higher in patients with CS-AKI with MOFthan inpatients with CS-AKI without MOF. In univariate analysis RRT-dependance at discharge of patients with CS-AKI with MOF was statistically significantly associated with arising of dopamine dose during thefirst day of RRT (p=0,006) and no-use of dobutamine at RRT start (p=0,006). Partial recovery was associated with early withdrawal of dobutamine (p=0,038).
Discussion. Frequency of renal recovery inpresented study was higher than in VA/NIHAcute Renal Failure Trial Network study because of differences inpatients'population and corresponds to otherstudies of CS-AKIpatients.
Conclusions. Recovery of renalfunction in CS-AKI patients associated with decreasing dose of dopamine, prolonged use ofdobutamine, illness severity and does not associated with specific RRT modality.