bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum miRNA–based Prediction of Axillary Lymph Node Metastasis in Breast Cancer
Authors: Shiino Sho , Matsuzaki Juntaro , Shimomura Akihiko , Kawauchi Junpei , Takizawa Satoko , Sakamoto Hiromi , Aoki Yoshiaki , Yoshida Masayuki , Tamura Kenji , Kato Ken , Kinoshita Takayuki , Kitagawa Yuko , Ochiya Takahiro 
Published: November 2018

Abstract

Purpose:
Sentinel lymph node biopsy (SLNB) is the gold-standard procedure for evaluating axillary lymph node (ALN) status in patients with breast cancer. However, the morbidity of SLNB is not negligible, and the procedure is invasive for patients without ALN metastasis. Here, we developed a diagnostic model for evaluating ALN status using a combination of serum miRNAs and clinicopathologic factors as a novel less-invasive biomarker.
Experimental Design: Preoperative serum samples were collected from patients who underwent surgery for primary breast cancer or breast benign diseases between 2008 and 2014. A total of 958 serum samples (921 cases of primary breast cancer, including 630 cases in the no ALN metastasis group and 291 cases in the ALN metastasis group, and 37 patients with benign breast diseases) were analyzed by miRNA microarray. Samples were randomly divided into training and test sets. Logistic LASSO regression analysis was used to construct diagnostic models in the training set, which were validated in the test set.

Results:
An optimal diagnostic model was identified using a combination of two miRNAs (miR-629-3p and miR-4710) and three clinicopathologic factors (T stage, lymphovascular invasion, and ultrasound findings), which showed a sensitivity of 0.88 (0.84–0.92), a specificity of 0.69 (0.61–0.76), an accuracy of 0.818, and an area under the receiver operating characteristic curve of 0.86 in the test set.

Conclusions:
Serum miRNA profiles may be useful for the diagnosis of ALN metastasis before surgery in a less-invasive manner than SLNB.
Batf3-Dependent Genes Control Tumor Rejection Induced by Dendritic Cells Independently of Cross-Presentation
Authors: Theisen Derek J. , Ferris Stephen T. , Briseño Carlos G. , Kretzer Nicole , Iwata Arifumi , Murphy Kenneth M. , Murphy Theresa L. 
Published: November 2018

Abstract
The BATF3-dependent cDC1 lineage of conventional dendritic cells (cDC) is required for rejection of immunogenic sarcomas and for rejection of progressive sarcomas during checkpoint blockade therapy. One unique function of the cDC1 lineage is the efficient cross-presentation of tumor-derived neoantigens to CD8+ T cells, but it is not clear that this is the only unique function of cDC1 required for tumor rejection. We previously showed that BATF3 functions during cDC1 lineage commitment to maintain IRF8 expression in the specified cDC1 progenitor. However, since cDC1 progenitors do not develop into mature cDC1s in Batf3−/− mice, it is still unclear whether BATF3 has additional functions in mature cDC1 cells. A transgenic Irf8-Venus reporter allele increases IRF8 protein concentration sufficiently to allow autonomous cDC1 development in spleens of Batf3−/− mice. These restored Batf3−/− cDC1s are transcriptionally similar to control wild-type cDC1s but have reduced expression of a restricted set of cDC1-specific genes. Restored Batf3−/− cDC1s are able to cross-present cell-associated antigens both in vitro and in vivo. However, Batf3−/− cDC1 exhibit altered characteristics in vivo and are unable to mediate tumor rejection. These results show that BATF3, in addition to regulating Irf8 expression to stabilize cDC1 lineage commitment, also controls expression of a small set of genes required for cDC1-mediated tumor rejection. These BATF3-regulated genes may be useful targets in immunotherapies aimed at promoting tumor rejection.
Two new species of phytoseid mites Euseius (Acari: Phytoseiidae) from Kerala, India
Authors: Santhosh P.P. , Sadanandan Mary Anithalatha , Rahul M.P. 
Published: November 2018

Two new species of phytoseiid mites, Euseius pariyarensis sp. nov. and E. curcasae sp. nov., collected from the medicinal plants Saraca indica L. and Jatropha curcas L. respectively, are described from the Kerala State of India. The morphological features of the two species are described with appropriate illustrations.
Aquatic and semi aquatic Hemiptera community of Sonebeel, the largest wetland of Assam, northeastern India
Authors: Saha Anupama , Gupta Susmita 
Published: November 2018

Aquatic and semiaquatic Hemiptera bugs play significant ecological roles, and they are important indicators and pest control agents. Little information is currently available concerning its populations in southern Assam. This study assessed hemipterans in four sites of Sonebeel, the largest wetland in Assam (3458.12 ha at full storage level), situated in Karimganj District. The major inflow and outflow of the wetland are the rivers Singla and Kachua, respectively (the Kachua drains into the Kushiyara River). Samples were trapped with pond nets and were seasonally recorded. This study recorded a total of 28 species of aquatic and semiaquatic hemipterans belonging to 20 genera under nine families. Population, geographical and environmental data (e.g., rainfall) were used to assess the relative abundance of species, species richness and different diversity indices, and species distribution.
A preliminary checklist of butterflies from the northern Eastern Ghats with notes on new and significant species records including three new reports for peninsular India
Authors: Goswami Rajkamal , Thorat Ovee , Aditya Vikram , Karimbumkara Seena Narayanan 
Published: November 2018

The northern Eastern Ghats is an area with significant biodiversity value but remains poorly explored except for a few charismatic taxa such as birds, mammals and reptiles. Very few studies have looked at the invertebrate diversity of these hill ranges, particularly butterflies. We present the first peer-reviewed checklist of butterflies from the northern Eastern Ghats based on a rapid and intensive survey carried out at five sites over 16 days across the buffer area of Papikonda National Park and Araku Valley in 2015 and 2016. We report a total of 102 species of butterflies from six lepidopteran families. Seventeen significant records include numerous first reports: three new species reports for peninsular India, nine for Eastern Ghats and 14 for the northern Eastern Ghats. This checklist adds 17 species to the known butterfly fauna for the state of Andhra Pradesh. It is hoped that findings from the study will help to mobilise conservation research, action and attention for the northern Eastern Ghats forest habitats, which are currently threatened by large scale development, security threats due to the Naxalite insurgency and mesoscale exploitation of forest resources.
The Juggler of Notre Dame and the Medievalizing of Modernity
Authors: Ziolkowski Jan M. 
Published: November 2018

This ambitious and vivid study in six volumes explores the journey of a single, electrifying story, from its first incarnation in a medieval French poem through its prolific rebirth in the nineteenth and twentieth centuries. The Juggler of Notre Dame tells how an entertainer abandons the world to join a monastery, but is suspected of blasphemy after dancing his devotion before a statue of the Madonna in the crypt; he is saved when the statue, delighted by his skill, miraculously comes to life.
Jan Ziolkowski tracks the poem from its medieval roots to its rediscovery in late nineteenth-century Paris, before its translation into English in Britain and the United States. The visual influence of the tale on Gothic revivalism and vice versa in America is carefully documented with lavish and inventive illustrations, and Ziolkowski concludes with an examination of the explosion of interest in The Juggler of Notre Dame in the twentieth century and its place in mass culture today. In this concluding volume, Ziolkowski explores the popularity of The Juggler of Notre Dame from the 1930s through the Second World War, especially in the Allied Resistance. Its popularity in the United States was subsequently maintained by figures as diverse as Tony Curtis and W. H. Auden, and although recently the story and medievalism have lost ground, the future of both holds promise.
Presented with great clarity and simplicity, Ziolkowski's work is accessible to the general reader, while its many new discoveries will be valuable to academics in such fields and disciplines as medieval studies, medievalism, philology, literary history, art history, folklore, performance studies, and reception studies.
Diagnostic and predictive performance of biomarkers in patients with sepsis in an intensive care unit
Authors: Zhang Yu , Khalid Sadia , Jiang Li 
Published: November 2018

Objective This study was performed to compare the predictive performance of serum procalcitonin (PCT), N-terminal brain natriuretic propeptide (NT-proBNP), interleukin-6 (IL-6), prothrombin time (PT), thrombin time (TT), and Sequential Organ Failure Assessment (SOFA) score in the intensive care unit (ICU). Methods This retrospective cohort study enrolled 150 patients with sepsis and septic shock and 30 control patients without sepsis. Each patient was followed until death or 28 days. Correlations between variables were assessed with Spearman’s rho test. The Kruskal–Wallis and Mann–Whitney U tests were used for between-group comparisons. Results Receiver operating characteristic curve analysis of the SOFA score, PCT, NT-proBNP, IL-6, PT, and TT showed an area under the curve of 0.872, 0.732, 0.711, 0.706, 0.806, and 0.691, respectively, for diagnosing sepsis. Binary logistic regression demonstrated that the SOFA score was an independent predictor of 28-day mortality and septic shock. The correlation coefficient (r) between SOFA and PCT, NT-proBNP and SOFA, IL-6 and SOFA, PT and SOFA, and TT and SOFA was 0.79, 0.52, 0.57, 0.56, and 0.58, respectively. Conclusion While the SOFA score is the gold standard, analysis of multiple biomarkers could increase the performance capacity for diagnosis and prognosis in patients with sepsis in the ICU.
Onset of menarche is not delayed in Slovenian patients with celiac disease
Authors: Mičetić-Turk Dušanka , Vlaisavljević Veljko , Turk Eva , Pogačar Maja Šikić 
Published: November 2018

Objective Celiac disease (CD) is an autoimmune disorder associated with numerous health problems, including reproductive disorders. This study was performed to analyze the association between CD and the menstrual cycle in a group of patients with CD and compare these patients’ characteristics with those of healthy women. Methods The study included 145 patients with CD (age, 15–51 years) and 162 healthy women (age, 18–55 years). Age at menarche and characteristics of the menstrual cycle were obtained by an anonymous questionnaire developed for the study. Results The age at onset of menarche was 12 to 14 years in 72.9% of the patients with CD and 77.3% of the healthy controls. For most patients (74.2%), the length of the menstrual cycle was around 27 to 28 days with 4 to 5 days of bleeding. Furthermore, 8.4% of patients versus 5.9% of controls experienced bleeding between cycles. Conclusions Our results suggest that in Slovenia, the age at menarche in patients with CD is 12.7 years, which is comparable with that in healthy women. We conclude that CD (treated or untreated) may not be associated with late menarche.
Regional and Facility Differences in Interventions for Mastitis by Australian Physiotherapists
Authors: Diepeveen Lara Clare , Fraser Elise , Croft Anna Jane Elizabeth , Jacques Angela , McArdle Adelle M. , Briffa Kathy , McKenna Leanda 
Published: November 2018

Background: Little information has been documented regarding interventions for mastitis by Australian physiotherapists. It is currently not known if physiotherapy interventions vary across Australian regions and types of healthcare facilities. Research aims: (1) To identify the interventions used by Australian physiotherapists treating mothers with mastitis and (2) to determine the variability in interventions used across regions and facilities. Methods: A retrospective observational design was used. A sample of case records of mothers with mastitis was identified ( N = 192). These case records documented physiotherapy interventions for mastitis in hospitals and private physiotherapy practices in Western Australia ( n = 77; 40.1%), Victoria ( n = 76; 39.6%), and New South Wales ( n = 39; 20.3%). An electronic data collection tool was designed to examine intervention variables. Results: The physiotherapy interventions received by mothers included therapeutic ultrasound (n=175; 91.1%), education and advice ( n = 160; 83.3%), and massage ( n = 103; 53.6%). Therapeutic ultrasound parameters varied across regions and types of healthcare facilities. Mean documented therapeutic ultrasound intensity was approximately twice as high in New South Wales and Victoria than in Western Australia. Conclusions: Regional and facility differences exist in physiotherapy interventions for mastitis in Australia. Healthcare professionals who refer to physiotherapists for mastitis should be aware that interventions received may differ across regions and facility types.
A Subjective Quality of Life Proxy for Older Adults in the PAQUID Cohort Study
Authors: Villeneuve Roxane , Meillon Céline , Bergua Valérie , Rascle Nicole , Dartigues Jean-François , Pérès Karine , Amieva Hélène 
Published: November 2018

Objectives: Quality of life is regarded as a major outcome in epidemiologic research, especially in the older population. Nevertheless, some cohort studies lack a specific instrument to evaluate it. The aim of this study was to propose a subjective quality of life proxy using easily accessible items, available in most epidemiologic studies. Method: We used data from the PAQUID (Personnes Agées Quid) cohort study (1991-1992, France). A subjective quality of life proxy was created based on items on positive affects, subjective health, and life satisfaction. Logistic and linear regression models as well as Cox survival models were used to assess the association between the proxy score and depression, dependence, cognitive complaints, adverse life events, comorbidities, and death. Analyses were replicated in an independent cohort study, AMI (Approche Multidisciplinaire intégrée; 2007-2008, France). All models were adjusted for age, sex, Mini-Mental State Examination score, and place of residence. Results: In the PAQUID sample (n = 2135), we found significant associations between the proxy score and the selected health outcomes. We found the same associations in the AMI cohort. Conclusion: This proxy might be useful when no gold standard for quality of life assessment is available or when in need of a short but reliable instrument that will not require extended administration time.
Prognostic significance of emergency department bypass in stable and unstable patients with ST-segment elevation myocardial infarction
Authors: Scholz Karl Heinrich , Friede Tim , Meyer Thomas , Jacobshagen Claudius , Lengenfelder Björn , Jung Jens , Fleischmann Claus , Moehlis Hiller , Olbrich Hans G , Ott Rainer , Elsässer Albrecht , Schröder Stephen , Thilo Christian , Raut Werner , Franke Andreas , Maier Lars S , Maier Sebastian KG 
Published: November 2018

Background: In ST-segment elevation myocardial infarction (STEMI) patients treated with percutaneous coronary intervention, direct transport from the scene to the catheterisation laboratory bypassing the emergency department has been shown to shorten times to reperfusion. The aim of this study was to investigate the effects of emergency department bypass on mortality in both haemodynamically stable and unstable STEMI patients. Methods: The analysis is based on a large cohort of STEMI patients prospectively included in the German multicentre Feedback Intervention and Treatment Times in ST-Elevation Myocardial Infarction (FITT-STEMI) trial. Results: Out of 13,219 STEMI patients who were brought directly from the scene by emergency medical service transportation and were treated with percutaneous coronary intervention, the majority were transported directly to the catheterisation laboratory bypassing the emergency department ( n=6740, 51% with emergency department bypass). These patients had a significantly lower in-hospital mortality than their counterparts with no emergency department bypass (6.2% vs. 10.0%, P<0.0001). The reduced mortality related to emergency department bypass was observed in both stable ( n=11,594, 2.8% vs. 3.8%, P=0.0024) and unstable patients presenting with cardiogenic shock ( n=1625, 36.3% vs. 46.2%, P<0.0001). Regression models adjusted for the Thrombolysis In Myocardial Infarction (TIMI) risk score consistently confirmed a significant and independent predictive effect of emergency department bypass on survival in the total study population (odds ratio 0.64, 95% confidence interval 0.56–0.74, P<0.0001) and in the subgroup of shock patients (OR 0.69, 95% CI 0.54–0.88, P=0.0028). Conclusion: In STEMI patients, emergency department bypass is associated with a significant reduction in mortality, which is most pronounced in patients presenting with cardiogenic shock. Our data encourage treatment protocols for emergency department bypass to improve the survival of both haemodynamically stable patients and, in particular, unstable patients. Clinical Trial Registration: NCT00794001 ClinicalTrials.gov: NCT00794001
Characterization of kaolin from Mankon, northwest Cameroon
Authors: Nzeukou Nzeugang A. , Ouahabi M. El , Aziwo B. , Mache J.R. , Mefire Mounton H.S. , Fagel N. 
Published: November 2018

ABSTRACTA kaolin deposit from Mankon (northwest Cameroon) was prospected and studied for potential applications in ceramics. Six samples were investigated with X-ray diffraction (XRD), infrared (IR) spectroscopy and scanning electron microscopy (SEM) to determine the mineralogical composition and with X-ray fluorescence (XRF) to determine the chemical composition and properties for ceramic applications. The main minerals in the clays are kaolinite/halloysite and anatase associated with alunite, illite, gibbsite and maghemite. The kaolin samples have abundant organic matter (4–10%) and low absorption of methylene blue (0.2–2.5 meq/100 g), while SiO2 (33.28–56.31%) and Al2O3 (19.26–35.87%) are major oxides. The particle-size distribution derived from sieving and the hydrometer method indicates that 12–38% of the samples are in the <2 μm clay fraction. The clays have low to moderate plasticity (7–21%). One sample with K-feldspar and plagioclase displays the necessary properties for red ceramic products. SEM confirmed the occurence of halloysite in sample M9. The high kaolinite/halloysite content (64–97%), associated with low Fe2O3 content (0.5–1.4%) demonstrates that five samples are suitable raw materials for white firing industrial kaolin.
Composition, technology and provenance of Roman pottery from Napoca (Cluj-Napoca, Romania)
Authors: Gál Ágnes , Ionescu Corina , Bajusz Mátyás , Codrea Vlad A. , Hoeck Volker , Barbu-Tudoran Lucian , Simon Viorica , Mureșan-Pop Marieta , Csók Zsolt 
Published: November 2018

ABSTRACTSecond-century CE (common era) household pottery sherds found in the city of Napoca (present day Cluj-Napoca, Romania) in Roman Dacia were investigated by polarized light optical microscopy, X-ray powder diffraction, Fourier-transform infrared spectroscopy and cold field emission scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy to obtain information on technology, raw materials and site of production. Compositionally, all samples are similar with comparable fine and semi-fine microstructures and oriented microtextures. Optically, there is a gradual transition from microcrystalline to an amorphous illitic-muscovitic matrix. The small aplastic inclusions are mostly quartz and feldspar. Fine-grained carbonate aggregates are distributed inhomogeneously in the ceramic body. Well-preserved Middle Miocene foraminifera tests are characteristic of the ceramics. The gradual thermal changes of the matrix and the newly formed phases upon firing, such as ‘ceramic melilite’, Fe-gehlenite, clinopyroxene, glass, hematite and some maghemite support inferences regarding the technological constraints in producing the pottery. The firing took place in a mostly oxidizing atmosphere and the temperature extended from at least 850°C to >900°C. The Middle Miocene marly clay from the area surrounding the site shows similar mineralogical and micropalaeontological contents to those of the ceramic specimens and is the best candidate for the raw material used for local production of the Roman pottery.
IL-15/IL-15Rα/CD80-expressing AML cell vaccines eradicate minimal residual disease in leukemic mice
Authors: Shi Yimin , Dincheva-Vogel Lillia , Ayemoba Charles E. , Fung Jeffrey P. , Bergamaschi Cristina , Pavlakis George N. , Farzaneh Farzin , Gaensler Karin M. L. 
Published: November 2018

AbstractEngineered autologous acute myeloid leukemia (AML) cells present multiple leukemia-associated and patient-specific antigens and as such hold promise as immunotherapeutic vaccines. However, prior vaccines have not reliably induced effective antileukemic immunity, in part because AML blasts have immune inhibitory effects and lack expression of the critical costimulatory molecule CD80. To enhance induction of leukemia-specific cytolytic activity, 32Dp210 murine AML cells were engineered to express either CD80 alone, or the immunostimulatory cytokine interleukin-15 (IL-15) with its receptor α (IL-15Rα), or heterodimeric IL-15/IL-15Rα together with CD80 and tested as irradiated cell vaccines. IL-15 is a γc-chain cytokine, with unique properties suited to stimulating antitumor immunity, including stimulation of both natural killer and CD8+ memory T cells. Coexpression of IL-15 and IL-15Rα markedly increases IL-15 stability and secretion. Non-tumor-bearing mice vaccinated with irradiated 32Dp210-IL-15/IL-15Rα/CD80 and challenged with 32Dp210 leukemia had greater survival than did mice treated with 32Dp210-CD80 or 32Dp210-IL-15/IL-15Rα vaccines, whereas no unvaccinated mice inoculated with leukemia survived. In mice with established leukemia, treatment with 32Dp210-IL-15/IL-15Rα/CD80 vaccination stimulated unprecedented antileukemic immunity enabling 80% survival, an effect that was abrogated by anti-CD8 antibody-mediated depletion in vivo. Because, clinically, AML vaccines are administered as postremission therapy, we established a novel model in which mice with high leukemic burdens were treated with cytotoxic therapy to induce remission (&lt;5% marrow blasts). Postremission vaccination with 32Dp210-IL-15/IL-15Rα/CD80 achieved 50% overall survival in these mice, whereas all unvaccinated mice achieving remission subsequently relapsed. These studies demonstrate that combined expression of IL-15/IL-15Rα and CD80 by syngeneic AML vaccines stimulates effective and long-lasting antileukemic immunity.
GVHD: biology matters
Authors: Ferrara James L. M. , Chaudhry Mohammed S. 
Published: November 2018

Abstract
Acute graft-versus-host disease (GVHD) targets the crypts in the gastrointestinal (GI) tract that are responsible for the self-renewal of the intestinal mucosa. Recent advances in the identification and culture of intestinal stem cells have improved our understanding of the interactions between the microbiome and the immune system (both innate and adaptive) that are key to the pathophysiology of GVHD. The identification of serum biomarkers that best predict long-term GVHD outcomes derive from the GI tract and have focused attention on cellular elements that act as shields against GVHD as well as its targets. These biomarkers have illuminated new mechanisms of crypt biology and provided insights that should prove useful both in the design of clinical trials and as guides to GVHD prevention and treatment.