bluuebunny/crossref_metadata_2025_split huggingface dataset:
A mechanism for ligand gated strand displacement in ZTP riboswitch transcription regulation
Authors: Strobel Eric J. , Cheng Luyi , Berman Katherine E. , Carlson Paul D. , Lucks Julius B. 
Published: January 2019

AbstractCotranscriptional folding is an obligate step of RNA biogenesis that can guide RNA structure and function by forming transient intermediate folds. This is especially true for transcriptional riboswitches in the which the formation of ligand-dependent structures during transcription regulates downstream gene expression. However, the intermediate structures that comprise cotranscriptional RNA folding pathways and the mechanisms that enable transit between them remain largely unknown. Here we determine the series of cotranscriptional folds and rearrangements that mediate antitermination by the Clostridium beijerinckii pfl riboswitch in response to the purine biosynthetic intermediate ZMP. We uncover sequence and structural determinants that modulate a regulatory RNA strand displacement reaction and identify biases within natural ZTP riboswitch sequences that promote on-pathway folding. Our findings establish a mechanism for ZTP riboswitch antitermination and suggest general strategies by which nascent RNA molecules can navigate cotranscriptional folding pathways efficiently.
Fibril formation rewires interactome of the Alzheimer protein Tau by π-stacking
Authors: Ferrari Luca , Stucchi Riccardo , Konstantoulea Aikaterini , Kamp Gerarda van de , Kos Renate , Geerts Willie J.C. , Förster Friedrich G. , Altelaar Maarten A.F. , Hoogenraad Casper C. , Rüdiger Stefan G.D. 
Published: January 2019

ABSTRACTAggregation of the Tau protein defines progression of neurodegenerative diseases, including Alzheimer’s Disease. Tau assembles into oligomers and fibrils. The molecular basis of their toxicity is poorly understood. Here we show that π-stacking by Arginine side chains rewires the interactome of Tau upon aggregation. Oligomeric nano-aggregates scavenge the COPI complex, fibrils attract proteins involved in microtubule binding, RNA binding and phosphorylation. The aberrant interactors have disordered regions with unusual sequence features. Arginines are crucial to initiate such aberrant interactions. Remarkably, substitution of Arginines by Lysines abolishes scavenging, which indicates a key role for the pi-stacking of the Arginine side chain. The molecular chaperone Hsp90 tames such re-arrangements, which suggests that the natural protein quality control system can suppress aberrant interactions. Together, our data present a molecular mode of action for derailment of protein-protein interaction in neurodegeneration.HIGHLIGHTSTau fibrils act as fishing net for proteins.Tau fibrils attract specific protein families associated with Alzheimer.π-stacking by Arginines key for aberrant binding to Tau fibrilsThe Hsp90 chaperone stalls fibril growth and alters interactome
The resin-flowing model in prepreg lamination of print circuit board
Authors: Liu Shitao , Cui Rong , Cao Hongwei , Qiu Jinhong 
Published: January 2019

Purpose
This paper aims to show a resin-flowing model based on Darcy’s law to display the flowing properties of prepreg during lamination. The conformity between the model and experimental results demonstrates that it can provide a guideline on print circuit board (PCB) lamination.

Design/methodology/approach
Based on the theoretical derivations of Darcy’s law, this paper made an analysis on the flow of prepreg in the pressing process, according to which a theoretical model, namely, resin-flowing model was further formulated.

Findings
This paper establishes a resin-flowing model, according to which two experiment-verified conclusions can be drawn: first, the resin-flowing properties of material A and B can be improved when the heating rate is between 1.5 and 2.5 min/°C; second, increased pressure gradient can add the amount of flowing resin, mainly featured by increasing pressure and reducing filled thickness of prepreg.

Originality/value
This model provides guidance on setting lamination parameters for most kinds of prepregs and decreasing starvation risk for PCB production.
Immigrant–native wage gap in Europe: the role of cognitive skills and their use at work
Authors: Tverdostup Maryna , Paas Tiiu 
Published: January 2019

PurposeThe purpose of this paper is to address the role of cognitive skills and extent of skill use at work in explaining the immigrant–native wage gap in Europe. The study targets immigrant–native disparities in literacy and numeracy cognitive skills, as important, yet not exhaustive factor behind immigrants’ wage penalty.Design/methodology/approachThe research relies on the Program of International Assessment of Adult Competencies data for 15 European countries. The empirical analysis employs multivariate regression analysis and incorporates the full set of plausible values for each skill domain, to correctly measure cognitive skills. To estimate standard errors, the authors employ Jackknife replication methodology with 80 replication weights and final population weight.FindingsThe authors document that, on average, immigrants achieve substantially worse scores in literacy and numeracy test domains. Only highly educated immigrants tend to improve their skills over time in host countries. The results of wage gap analysis indicate that having cognitive skills, demographic profile and occupation category comparable to natives does not yield comparable wage rate. The remaining wage gap results from the systematic differences in skills application at work, as immigrants use their skills to lower extent, relative to natives.Originality/valueThe research employs a novel measure of productive human capital, which accounts for cognitive skills in literacy and numeracy domains, and frequencies of skill use at work. It allows to more precisely evaluate the immigrant–native disparity in human capital application and its reflection on the wage rate.
Can pay gaps between gay men and lesbians shed light on male–female pay gaps?
Authors: Wang Jing , Gunderson Morley 
Published: January 2019

PurposeThe purpose of this paper is to estimate the relative importance of gender discrimination and differences in household responsibilities as determinants of the male–female pay gap.Design/methodology/approachIt parses out the relative importance of those two factors by using the pay between gay men vs lesbian women as a comparison group that should reflect only gender discrimination. Subtracting the pay gap between gay men and lesbians (reflecting only gender discrimination) from the male–female pay gap for their heterosexual counterparts (reflecting both gender discrimination and household responsibilities) provides evidence of the relative importance of gender discrimination and household responsibilities in explaining the male–female pay gap.FindingsThe results show that essentially all of the male–female pay gap is attributed to differences in household responsibilities.Originality/valueThis paper advances the literature of gender wage gap by using a novel comparison group – gay men vs lesbian women – to estimate the relative importance of gender discrimination and differences in household responsibilities as determinants of the male–female pay gap.
Does foreign aid help alleviate income inequality? New evidence from African countries
Authors: Younsi Moheddine , Khemili Hasna , Bechtini Marwa 
Published: January 2019

PurposeThe purpose of this paper is to examine the relationship between foreign aid and income inequality (IIQ) reduction for 16 African countries using unbalanced panel data covering the period 1990–2011. This paper attempts to answer the critical question: does foreign aid lead to IIQ reduction?Design/methodology/approachTo examine the effect of foreign aid on IIQ, this paper uses an RE model with robust OLS regression and system-GMM estimator, which are useful in dealing with the endogeneity problems.FindingsResults of RE model indicate that foreign aid, foreign direct investment, trade openness as well as corruption have a positive and statistically significant effect on IIQ. Government spending and inflation have a negative and statistically significant effect on IIQ, while GDP per capita growth has a negative but statistically insignificant relationship with IIQ. The results are robust by using system-GMM dynamic panel model which confirms that the coefficients of all considered variables remain same sign and significance.Research limitations/implicationsThis study implies that an increase in foreign aid is associated with an increase in IIQ. As an effective strategy to foreign aid, this paper suggests that improving of financial sector development, and institutional quality and policies can reduce income inequalities and stimulate economic growth.Originality/valueThis paper is the first of its kind to empirically explore the relationship between IIQ and foreign aid measured here by net aid transfers as a share of GDP in African countries, using modern econometric techniques, time period and a variety of control variables.
Strategic alliances and dynamic capabilities: a systematic review
Authors: Mamédio Diorgenes , Rocha Clarissa , Szczepanik Dayanne , Kato Heitor 
Published: January 2019

Purpose
Organizations need to find ways to survive in unpredictable, dynamic and rapidly changing environments. The development of organizational capabilities, such as relational, is a dynamic process of adaptation, which seeks to adjust to an unstable environment. Within this context, the purpose of this paper is to analyze, through a systematic review (SR), 36 articles, which discuss the influence of strategic alliances on the dynamic capabilities (DCs) process.

Design/methodology/approach
Through this systematic literature review, articles published in international journals were categorized in terms of their objectives, thematic axes and the research methodologies applied and evidences, to explore their methodological, theoretical and organizational practice contributions.

Findings
The results clarified the antecedents, implications and relevance of the relationship between strategic alliances in different countries and their DCs. The findings make it possible to determine directions for new studies that may support the advancement of this field of research.

Research limitations/implications
Studies from this SR point out that alliance is: a flexible vehicle of learning; a way to transfer effective knowledge in partner firms and to generate combinations of resources; and a superior means of access to technological capabilities and other complex capabilities. Those aspects would be difficult to be developed in the firms without the help of partnerships. This can encourage the reconfiguration of existing resources and capabilities. These reconfigurations can lead to deliberate evolutionary adaptations in these firms, which will build relational DCs.

Practical implications
As managerial implications, an alliance allows for the development of capabilities to detect new opportunities, and for the development of the reconfiguration or expansion of the existing resource base in the firm. Thus, alliances, once properly managed and with well-defined structure and purpose, can help firms achieve a sustainable competitive advantage. Alliances can serve as a type of strategic choice or an alternative that can enable companies to cope with unstable, global and competitive environments permeated by (new) threats and opportunities.

Originality/value
This SR paper highlighted, in a specific and consistent topic, the suggestions for future research related to the process of DCs and its relationship with strategic alliances, motivating new research works.
The effects of the winery visitor experience on emotions, satisfaction and on post-visit behaviour intentions
Authors: Leri Ifigeneia , Theodoridis Prokopis 
Published: January 2019

Purpose
This paper aims to explore the impact of the experience of a winery visit to the visitor’s emotions and the effect of these emotions on visitor’s intention to revisit and recommend a winery as consequences of visitor satisfaction.

Design/methodology/approach
The study adopted a multi approach of customer experience, suggesting that visitors base their experience perception on the servicescape attributes, other visitors’ suitable behaviours and their own yearn for cognitive learning and fun. Path analysis is adopted to measure the impact of these constructs on visitor’s emotions and the role of these emotions in predicting visitor’s satisfaction and behavioural intentions. Data are collected through a self-administered highly structured questionnaire, self-completed by respondents at each winery. A total of 615 usable responses are obtained from eight wineries.

Findings
The results indicate that emotions are arisen – in a context of wine tourism – as a result of visitor’s evaluation of winery environment (especially Atmospherics, Signage and Facilities), other visitors’ appropriate behaviour and visitor’s desire to learn something new and to have fun during the winery experience. Also, it is revealed that visitor’s emotions positively impact on his/her satisfaction, which in turn seems to be a significant predictor of his/her intention to revisit the winery and give positive word-of-mouth.

Research limitations/implications
The findings broaden the knowledge on visitor’s winery experience and its impact on visitor’s satisfaction and future behavioural intentions. However, because the study focuses only on winery visitors in Greece, the research results may lack generalizability. The application of the study to other wineries would allow for wider generalisations to be made from the results obtained.

Practical implications
The overall findings may have significant implications for wine tourism industries. Also, it might be beneficial for wineries in their effort to provide a more valuable experience to wine tourists and in increasing wine tourists’ loyalty.

Originality/value
The paper’s originality lies in providing information to clarify the relationships between the constructs of experience, visitor’s emotions, satisfaction and future behavioural intentions in a winery setting. Also, this study provides new and practical insights of winery experience in the Greek context, an area where very limited research has been conducted. Finally, this is one of the few studies that focus on more than two servicescape elements and their impact on visitor’s emotions.
Coordination of Fe(II) to Eugenol to Engineer Self‐Assembled Emulsions by Rice Proteins for Iron Fortification
Authors: Wang Ren , Xu Pengcheng , Yue Ming , Chen Zhengxing , Wang Tao 
Published: January 2019

AbstractFortification of ferrous irons (FeII) is challenging due to fast oxidation. In this paper, we report that the coordination of FeII to eugenol can effectively inhibit the oxidation of FeII. The coordination was studied by UV‐Vis spectra, nuclear magnetic resonance, Raman spectra, and so on, showing that it took less than 1 hr for FeII to be fully oxidized in water, whereas more than 10 days in eugenol. In addition, by gently mixing FeII coordinated eugenol and modified rice protein (MRP) solutions at ambient temperature, core‐shell structures <200 nm were spontaneously formed, which can be utilized as protective and delivery systems for the organic ferrous irons. Based on the simulated digestion of MRPs, the FeII loaded emulsions may be delivered into intestinal tract beyond 2‐hr oral administration. The results highlight the effectiveness of fortification of ferrous irons via polyphenol coordination combined with self‐emulsification, advancing the future oral fortification of ferrous irons towards a feasible approach.Practical ApplicationThe coordination of metal ions with food polyphenols can provide the functional food with excellent redox stability. The FeII loaded eugenol emulsified by MRPs can be used as a straightforward protocol for the development of functional ingredients for iron fortification in food industry.
An in situ approach for measuring biogeochemical fluxes in structurally complex benthic communities
Authors: Roth Florian , Wild Christian , Carvalho Susana , Rädecker Nils , Voolstra Christian R. , Kürten Benjamin , Anlauf Holger , El‐Khaled Yusuf C. , Carolan Ronan , Jones Burton H. 
Published: January 2019

Abstract

The exchange of energy and nutrients are integral components of ecological functions of benthic shallow‐water ecosystems and are directly dependent on in situ environmental conditions. Traditional laboratory experiments cannot account for the multidimensionality of interacting processes when assessing metabolic rates and biogeochemical fluxes of structurally complex benthic communities. Current in situ chamber systems are expensive, limited in their functionality and the deployment is often restricted to planar habitats (e.g. sediments or seagrass meadows) only.

To overcome these constraints, we describe a protocol to build and use non‐invasive, cost‐effective and easy to handle in situ incubation chambers that provide reproducible measurements of biogeochemical processes in simple and structurally complex benthic shallow‐water communities. Photogrammetry tools account for the structural complexity of benthic communities, enabling to calculate accurate community fluxes. We tested the performance of the system in laboratory assays and various benthic habitats (i.e. algae growing on rock, coral assemblages, sediments and seagrass meadows). In addition, we estimated community budgets of photosynthesis and respiration by corals, rock with algae and carbonate sediments, which were subsequently compared to budgets extrapolated from conventional ex situ single‐organism incubations.

The tests highlight the transparency (>90% light transmission) of the chambers and minimal water exchange with the surrounding medium on most substrates. Linear dissolved oxygen fluxes in dependence to incubation time showed sufficient mixing of the water by circulation pumps and no organismal stress response. The comparison to single‐organism incubations showed that ex situ measurements might overestimate community‐wide net primary production and underestimate respiration and gross photosynthesis by 20%–90%.

The proposed protocol overcomes the paucity of observational and manipulative studies that can be performed in in situ native habitats, thus producing widely applicable and realistic assessments on the community level. Importantly, the tool provides a standardized approach to compare community functions across a wide range of benthic habitats. We identify multiple experimental strategies, including the manipulation of stressors/factors, and discuss how the method may be implemented in a variety of aquatic studies.
The stop‐flow arm equilibrium pressure in preoperative patients: Stressed volume and correlations with echocardiography
Authors: Yastrebov Konstantin , Aneman Anders , Slama Michel , Kokhno Vladimir , Luchansky Vsevolod , Orde Sam , Hilton Andrew , Lukiyanov Dmitriy , Volobueva Irina , Sidelnikova Svetlana , Polovnikov Evgeniy 
Published: January 2019

BackgroundThe distending intravascular pressure at no flow conditions reflects the stressed volume. While this haemodynamic variable is recognised as clinically important, there is a paucity of reports of its range and responsiveness to volume expansion in patients without cardiovascular disease and no reports of correlations to echocardiographic assessments of left ventricular filling.MethodsTwenty‐seven awake (13 male), spontaneously breathing patients without any history of cardiopulmonary, vascular or renal disease were studied prior to induction of anaesthesia. The no‐flow equilibrium pressure in the arm following rapid circulatory occlusion (Parm) was measured via a radial arterial catheter. Transthoracic echocardiography was used to measure left ventricular end diastolic area and volume as well as the diameter of the inferior vena cava. The Parm and echocardiographic variables were measured before and after administration of 500 mL 0.9% NaCl over 10 minutes. Changes were analysed by paired t test, Pearson's correlation and multiple linear regression.ResultsParm increased overall from 22 ± 5 mm Hg to 25 ± 6 mm Hg (mean difference 3.0 ± 4.5 mm Hg, P = 0.002) following the fluid bolus with corresponding increases in arterial pressure and echocardiographic variables. Variability in the direction of the Parm response reflected concomitant changes in vascular compliance. Only weak correlations were observed between changes in Parm and inferior vena cava diameter indexed to body surface area (R2 = 0.29, P = 0.01).ConclusionPreoperative measurements of Parm increased following acute expansion of the intravascular volume. Echocardiography demonstrated poor correlation with Parm.
Meta‐analyses of nest predation in temperate Australian forests and woodlands
Authors: Fulton Graham R. 
Published: January 2019

AbstractNest predation is the leading cause of nesting failure. Thus it is a crucial area of research needed to inform conservation management and to understand the life history of birds. I surveyed the literature to review the identity of nest predators and the factors affecting nest predation, in Australia using 177 studies. Overall, 94 nest predators were identified when incorporating artificial nests, 69 without. Using only natural nests, the Pied Currawong Strepera graculina was the most frequently reported nest predator. Five nest predators, including Pied Currawong, depredated 40% of the prey measured by the number of prey species taken. Yet, 60% of predation was carried out by the other 64 species, which included by the order of importance birds, mammals, reptiles, frogs and ants. Predation at cup and dome nests was more frequently reported than at burrow, ground and hollow nests. Only 28% of predators were observed at both artificial and natural nests suggesting artificial nests have limited, but not negligible, ability as tools for identifying predators. There was a highly significant and positive correlation between predator and prey masses. The predator prey mass ratio was calculated with a mean 0.25 and a median 0.22, a result closely matching with the proportional size of prey taken by raptors. The finding that predator size is proportional to prey opens a pathway for more life history and conservation research.