bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proximity labeling reveals interactions necessary to maintain the distinct apical domains of Drosophila photoreceptors
Authors: Sastry Lalitha , Rylee Johnathan , Mahato Simpla , Zelhof Andrew C. 
Published: November 2024

ABSTRACT
Specialized membrane and cortical protein regions are common features of cells and are utilized to isolate differential cellular functions. In Drosophila photoreceptors, the apical membrane domain is defined by two distinct morphological membranes: the rhabdomere microvilli and the stalk membrane. To define the apical cortical protein complexes, we performed proximity labeling screens utilizing the rhabdomeric-specific protein PIP82 as bait. We found that the PIP82 interactome is enriched in actin-binding and cytoskeleton proteins, as well as proteins for cellular trafficking. Analysis of one target, Bifocal, with PIP82 revealed two independent pathways for localization to the rhabdomeric membrane and an additional mechanism of crosstalk between the protein complexes of the rhabdomeric and stalk membranes. The loss of Bifocal, and enhancement in the PIP82, bifocal double mutant, resulted in the additional distribution of Crumbs, an apical stalk membrane protein, to the lateral basal photoreceptor membrane. This phenotype was recapitulated by the knockdown of the catalytic subunit of Protein phosphatase 1, a known interactor with Bifocal. Taken together, these results expand our understanding of the molecular mechanisms underlying the generation of the two distinct photoreceptor apical domains.
A New Multicommodity Network Flow Model and Branch and Cut for Optimal Quantum Boolean Circuit Synthesis
Authors: Jung Jihye , Choi In-Chan 
Published: November 2024

This study introduces a new optimization model and a branch-and-cut approach for synthesizing optimal quantum circuits for reversible Boolean functions, which are pivotal components in quantum algorithms. Although heuristic algorithms have been extensively explored for quantum circuit synthesis, research on exact counterparts remains relatively limited. However, the need to design quantum circuits with guaranteed optimality is increasing, especially for improving computational fidelity on noisy intermediate-scale quantum devices. This study presents mathematical optimization as a viable option for optimal synthesis, with the potential to accommodate practical considerations arising in fast-evolving quantum technologies. We set a demonstrative problem to implement reversible Boolean functions using high-level gates known as multiple control Toffoli gates while minimizing a technology-based proxy called quantum cost—the number of low-level gates used to realize each high-level gate. To address this problem, we propose a discrete optimization model based on a multicommodity network and discuss potential future variations at an abstract level to incorporate technical considerations. A customized branch and cut is then developed upon different aspects of our model, including polyhedron integrality, surrogate constraints, and variable prioritization. Our experiments demonstrate the robustness of the proposed approach in finding cost-optimal circuits for all benchmark instances within a two-hour time frame. Furthermore, we present interesting intuitions from these experiments and compare our computational results with relevant studies, highlighting newly discovered circuits with the lowest quantum costs reported in this paper. History: Accepted by Giacomo Nannicini, Area Editor for Quantum Computing. This paper has been accepted for the INFORMS Journal on Computing Special Issue on Quantum Computing. Funding: This research was supported by the Ministry of Science and Information and Communication Technology, South Korea [Grants 2017R1E1A1A0307098814 and 2020R1A4A307986411]. Supplemental Material: The software that supports the findings of this study is available within the paper and its Supplemental Information ( https://pubsonline.informs.org/doi/suppl/10.1287/ijoc.2024.0562 ) as well as from the IJOC GitHub software repository ( https://github.com/INFORMSJoC/2024.0562 ). The complete IJOC Software and Data Repository is available at https://informsjoc.github.io/ .
Hemeroteca: um lugar de memória do jornalismo de Picos-PI
Authors: Sousa de Oliveira Thamyres , Machado de Sousa Paloma Aparecida , Santos Vieira Thaila Vitoria , Da Silva Modesto Marcos Vinicius , De Sousa Silva Beatriz 
Published: November 2024

A extensão tem em seu cerne a missão de transformar e fazer com que a academia e a comunidade andem lado a lado. Desse modo, neste relato de experiência, buscamos compartilhar como o projeto Hemeroteca: um lugar de memória do jornalismo de Picos-PI tem engendrado um lugar de memória do jornalismo na cidade. A metodologia utilizada no projeto, conforme Warschauer (2017), se concentra em três círculos principais: 1) planejamento, formação, acompanhamento e avaliação da organização do acervo de memória; 2) garimpagem de publicações jornalísticas e organização do acervo físico; 3) educação para a preservação da memória por meio da realização de minicursos. O projeto tem estimulado o protagonismo discente e mostrado que a educação se constrói também pela afetividade.
THE ROLE OF REPEAT POSTOPERATIVE RADIOGRAPHS IN PREDICTING REVISION SURGERY FOLLOWING PRIMARY KNEE ARTHROPLASTY USING MACHINE LEARNING
Authors: Panagiota Glynou S. , Musbahi O. , Cobb J. 
Published: November 2024

IntroductionKnee arthroplasty (KA), encompassing Total Knee Replacement (TKR) and Unicompartmental Knee Replacement (UKR), is one of the most common orthopedic procedures, aimed at alleviating severe knee arthritis. Postoperative KA management, especially radiographic imaging, remains a substantial financial burden and lacks standardised protocols for its clinical utility during follow-up.MethodIn this retrospective multicentre cohort study, data were analysed from January 2014 to March 2020 for adult patients undergoing primary KA at Imperial NHS Trust. Patients were followed over a five-year period. Four machine learning models were developed to evaluate if post-operative X-ray frequency can predict revision surgery. The best-performing model was used to assess the risk of revision surgery associated with different number of X-rays.ResultThe study assessed 289 knees with a 2.4% revision rate. The revision group had more X-rays on average than the primary group. The best performing model was Logistic Regression (LR), which indicated that each additional X-ray raised the revision risk by 52% (p<0.001). Notably, having four or more X-rays was linked to a three-fold increase in risk of revision (OR=3.02; p<0.001). Our results align with the literature that immediate post-operative X-rays have limited utility, making the 2nd post-operative X-ray of highest importance in understanding the patient's trajectory. These insights can enhance management by improving risk stratification for patients at higher revision surgery risk. Despite LR being the best-performing model, it is limited by the dataset's significant class imbalance.ConclusionX-ray frequency can independently predict revision surgery. This study provides insights that can guide surgeons in evidence-based post-operative decision-making. To use those findings and influence post-operative management, future studies should build on this predictive model by incorporating a more robust dataset, surgical indications, and X-ray findings. This will allow early identification of high-risk patients, allowing for personalised post-operative recommendations.
THE ROLE OF THE PLANTAR FASCIA IN SUPPORTING THE ARCH IN PROGRESSIVE COLLAPSING FOOT DEFORMITY
Authors: Malakoutikhah H. , Madenci E. , Latt D. 
Published: November 2024

IntroductionThe arch of the foot has been described as a truss where the plantar fascia (PF) acts as the tensile element. Its role in maintaining the arch has likely been underestimated because it only rarely torn in patients with progressive collapsing foot deformity (PCFD). We hypothesized that elongation of the plantar fascia would be a necessary and sufficient precursor of arch collapse.MethodWe used a validated finite element model of the foot reconstructed from CT scan of a female cadaver. Isolated and combined simulated ligament transection models were created for each combination of the ligaments. A collapsed foot model was created by simulated transection of all the arch supporting ligaments and unloading of the posterior tibial tendon. Foot alignment angles, changes in force and displacement within each of the ligaments were compared between the intact, isolated ligament transection, and complete collapse conditions.ResultIsolated release of the PF did not cause deformity, but lead to increased force in the long (142%) and short plantar (156%), deltoid (45%), and spring ligaments (60%). The PF was the structure most able to prevent arch collapse and played a secondary role in preventing hindfoot valgus and forefoot abduction deformities. Arch collapse was associated with substantial attenuation of the spring (strain= 41%) and interosseous talocalcaneal ligaments (strain= 27%), but only a small amount in the plantar fascia (strain= 10%).ConclusionIsolated PF release did not cause arch collapse, but arch collapse could not occur without at least 10% elongation of the PF. Simulated transection of the PF led to substantial increase in the force in the other arch supporting ligaments, putting the foot at risk of arch collapse over time. Chronic degeneration of the PF leading to plantar fasciitis may be an early sign of impending PCFD.
PROGRESSIVE RESISTANCE TRAINING OR NEUROMUSCULAR EXERCISE FOR HIP OSTEOARTHRITIS: A MULTICENTRE CLUSTER RANDOMIZED CONTROLLED TRIAL
Authors: Kjeldsen T. , Thorgaard Skou S. , Dalgas U. , Tønning L. , Birch S. , Frydendal T. , Varnum C. , Garval M. , G Ingwersen K. , Mechlenburg I. 
Published: November 2024

IntroductionExercise is recommended as first-line treatment for patients with hip osteoarthritis (OA). Interestingly, content and dose of exercise interventions seem to be important for the effect of exercise interventions, but the optimal content and dose is unknown. This warrants randomized controlled trials providing evidence for the optimal exercise program in Hip OA. The aim of this trial was to investigate whether progressive resistance training (PRT) is superior to neuromuscular exercise (NEMEX) for improving functional performance, hip pain and hip-related quality of life in patients with hip OA.MethodThis was a multicenter, cluster-randomized, controlled, parallel-group, assessor-blinded, superiority trial. 160 participants with clinically diagnosed hip OA were recruited from hospitals and physiotherapy clinics and randomly assigned to twelve weeks of PRT or NEMEX. The PRT intervention consisted of 5 high-intensity resistance training exercises targeting muscles at the hip and knee joints. The NEMEX intervention included 10 exercises and emphasized sensorimotor control and functional stability. The primary outcome was change in the 30-second chair stand test (30s-CST). Key secondary outcomes were changes in scores on the pain and hip-related quality of life (QoL) subscales of the Hip Disability and Osteoarthritis Outcome Score (HOOS).ResultThe mean changes from baseline to 12-week follow-up in the 30s-CST were 1.5 (95% CI, 0.9 to 2.1) chair stands with PRT and 1.5 (CI, 0.9 to 2.1) chair stands with NEMEX (difference, 0.0 [CI, 0.8 to 0.8] chair stands). For the HOOS pain subscale, mean changes were 8.6 (CI, 5.3 to 11.8) points with PRT and 9.3 (CI, 5.9 to 12.6) points with NEMEX. For the HOOS QoL subscale, mean changes were 8.0 (CI, 4.3 to 11.7) points with PRT and 5.7 (CI, 1.9 to 9.5) points with NEMEX.ConclusionIn patients with hip OA, PRT is not superior to NEMEX for improving functional performance, hip pain, or hip-related QoL.
CAN PIN SITE INFLAMMATION BE DETECTED WITH THERMOGRAPHIC IMAGING? A CROSS-SECTIONAL MULTICENTRE STUDY OF PATIENTS TREATED WITH EXTERNAL FIXATORS
Authors: Fridberg M. , Rahbek O. , Husum H.-C. , Bafor A. , Duch K. , Iobst C. , Kold S. 
Published: November 2024

IntroductionPatients with external fixators are at risk of pin site infection. A more objective assessment of possible pin site infection is warranted, particularly for future home-based monitoring of pin sites. The aim was to determine if thermography can detect signs of inflammation around pin sites by 1) Establishing a maximum temperature cut-off value 2) Investigating the correlation between local temperature and visual signs of inflammation 3) Adjust for anatomical location and ambient room temperature.MethodThis was a cross-sectional international multi-center study following STROBE guidelines. All patients with external ring-fixators scheduled for a visit in the out-patient clinic were eligible. Visual signs of inflammation were categorized using the Modified Gordon classification System (MGS, simplified sMGS). Thermographic imaging was done with an infrared camera (FLIR T540) and the maximum temperature within the ROI (MaxTp) was the primary outcome measure. Sample size and reliability were estimated. Cohen-Kappa, ROC-curve/AUC and Poisson regression were used for statistical analysis.ResultData from 1970 pin sites were included. Inter-rater reliability of MGS was Kappa=0.79 and for MaxTp ICC=0.99 (95%CI: 0.99;0.99). Overall, a tendency of rising temperature with increasing sMGS was seen. The difference between sMGS=0 and sMGS>0 was significant. The performance of MaxTp as a screening tool to detect inflammation was reasonable with an AUC of 0.71 (95% CI: 0.65-0.76). The empirically optimal cut-off value was 34.1°C (Sensitivity=65%, Specificity=72%, Positive predictive value=23%, Negative Predictive value=94%). A 1°C increase in MaxTp increased the RR of visual signs of inflammation by a factor 1.5 (95% CI: 1.3; 1.7).ConclusionWe found a clinical positive association between the temperature at the pin site measured with thermography and visual signs of inflammation. The empirically optimal temperature cut-off value for inflammation screening was 34.1°C. Thermography may be a promising tool for a for a future point of care technology.
FEASIBILITY OF VIRTUAL REALITY IMMERSION IN REDUCING PAIN AND ANXIETY DURING EXTERNAL FIXATOR CARE PROCEDURES
Authors: Bafor A. , Lobst C. 
Published: November 2024

IntroductionPrevious studies have shown the potential for virtual reality (VR) immersion as a promising technique for pain and anxiety management. The aim of our study was to evaluate the feasibility of VR in the management of pain and anxiety during post-op external fixator care procedures.MethodThis study involved patients aged 5-21 years following limb lengthening/reconstruction surgery with an external fixator. Aqua VR application from the KindVR® was utilized for this study. Subjects were seen during the first four postoperative visits and assigned to a ‘VR-first’ or ‘no-VR-first’ group. Visits alternated between VR immersion and no VR immersion during care procedures. The study endpoints (pain and anxiety levels) were assessed before, during, and after procedures using the Wong-Baker Faces (FACES) and Children's Fear Scale, respectively. Proxy scores for pain and anxiety were also obtained from parents or legal guardians and providers.ResultA total of 29 patients (16 male and 13 female) were evaluated. The mean age at enrollment was 14.4 ± 2.2 years for group 1 and 14.7 ± 4.0 years for group 2. The median number of pin sites was 7. Anxiety scores were consistently higher during the non-VR immersion experience compared to the VR immersion visits. The pain and anxiety scores were significantly lower in the ‘VR-first’ group during the non-VR immersion study visits compared to patients in the ‘no-VR-first’ group. This observation was also consistent with survey findings among the parent proxies and providers.ConclusionVR immersion is associated with lower anxiety scores for pin-site care procedures. VR immersion at the first post-operative visit following limb reconstruction surgery was also associated with lower pain and anxiety scores during subsequent non-VR immersion visits.
PATIENT-SPECIFIC CALIBRATION OF PREOPERATIVE CT SCANS PROVIDE OBJECTIVE CLASSIFICATION OF BONE DENSITY SUBGROUPS FROM PATIENTS UNDERGOING REVERSE SHOULDER ARTHROPLASTY
Authors: Ritter D. , Bachmaier S. , Wijdicks C. , Raiss P. 
Published: November 2024

IntroductionThe increased prevalence of osteoporosis in the patient population undergoing reverse shoulder arthroplasty (RSA) results in significantly increased complication rates. Mainly demographic and clinical predictors are currently taken into the preoperative assessment for risk stratification without quantification of preoperative computed tomography (CT) data (e.g. bone density). It was hypothesized that preoperative CT bone density measures would provide objective quantification with subsequent classification of the patients’ humeral bone quality.MethodsThirteen bone density parameters from 345 preoperative CT scans of a clinical RSA cohort represented the data set in this study. The data set was divided into testing (30%) and training data (70%), latter included an 8-fold cross validation. Variable selection was performed by choosing the variables with the highest descriptive value for each correlation clustered variables. Machine learning models were used to improve the clustering (Hierarchical Ward) and classification (Support Vector Machine (SVM)) of bone densities at risk for complications and were compared to a conventional statistical model (Logistic Regression (LR)).ResultsClustering partitioned this cohort (training data set) into a high bone density subgroup consisting of 96 patients and a low bone density subgroup consisting of 146 patients. The optimal number of clusters (n = 2) was determined based on optimization metrics. Discrimination of the cross validated classification model showed comparable performance for the training (accuracy=91.2%; AUC=0.967) and testing data (accuracy=90.5 %; AUC=0.958) while outperforming the conventional statistical model (Logistic Regression (LR)). Local interpretable model-agnostic explanations (LIME) were created for each patient to explain how the predicted output was achieved.ConclusionThe trained and tested model provides preoperative information for surgeons treating patients with potentially poor bone quality. The use of machine learning and patient-specific calibration showed that multiple 3D bone density scores improved accuracy for objective preoperative bone quality assessment.
INTEGRIN Α10Β1-SELECTED HUMAN MESENCHYMAL STEM CELLS CAN DIRECTLY PARTICIPATE IN CARTILAGE REGENERATION AND HAVE IMMUNOMODULATORY CAPACITIES
Authors: Uvebrant K. , Andersen C. , Lim H. C. , Vonk L. , Åkerlund E. L. 
Published: November 2024

IntroductionHomogenous and consistent preparations of mesenchymal stem cells (MSCs) can be acquired by selecting them for integrin α10β1 (integrin a10-MSCs). Safety and efficacy of intra-articular injection of allogeneic integrin a10-MSCs were shown in two post-traumatic osteoarthritis horse studies. The current study investigated immunomodulatory capacities of human integrin a10-MSCs in vitro and their cell fait after intra-articular injection in rabbits.MethodThe concentration of produced immunomodulatory factors was measured after licensing integrin a10-MSCs with pro-inflammatory cytokines. Suppression of T-cell proliferation was determined in co-cultures with carboxyfluorescein N-succinimidyl ester (CFSE) labelled human peripheral blood mononuclear cells (PBMCs) stimulated with anti-CD3/CD28 and measuring the CFSE intensity of CD4+ cells. Macrophage polarization was assessed in co-cultures with differentiated THP-1 cells stimulated with lipopolysaccharide and analysing the M2 macrophage cell surface markers CD163 and CD206. In vivo homing and regeneration were investigated by injecting superparamagnetic iron oxide nanoparticles conjugated with Rhodamine B-labeled human integrin a10-MSCs in rabbits with experimental osteochondral defects. MSC distribution in the joint was followed by MRI and fluorescence microscopy.ResultThe production of the immunomodulatory factors indoleamine 2,3-dioxygenase and prostaglandin E2 was increased after inflammatory licensing integrin a10-MSCs. Co-cultures with integrin a10-MSCs suppressed T-cell proliferation and increased the frequency of M2 macrophages. In vivo injected integrin a10-MSCs homed to osteochondral defects and were detected in the repair tissue of the defects up to 10 days after injection, colocalized with aggrecan and type II collagen.ConclusionThis study showed that human integrin a10-MSCs have immunomodulatory capacities and in vivo can home to the site of osteochondral damage and directly participate in cartilage regeneration. This suggests that human integrin α10β1-selected MSCs may be a promising therapy for osteoarthritis with dual mechanisms of action consisting of immunomodulation and homing to damage followed by early engraftment and differentiation into chondrocyte-like cells that deposit hyaline cartilage matrix molecules.
MULTIFACTORIAL BIOENGINEERING TO CONTROL CELL FATE
Authors: Zevgolis D. 
Published: November 2024

IntroductionBereft of their optimal tissue context, cells lose their phenotype, function and therapeutic potential during in vitro culture. Despite the fact that in vivo cells are exposed simultaneously to multiple signals, traditional ex vivo cultures are monofactorial. With these in mind, herein we assessed the combined effect of surface topography, substrate rigidity, collagen type I coating and macromolecular crowding in human tenocyte, skin fibroblast and bone marrow mesenchymal stromal cell cultures.MethodsThermal imprinted was used to pattern (groove depth: 2,000 nm, groove width: 2,000 nm, line width: 2,000 nm) polydimethylsiloxane substrates of different rigidity (50 kPa, 130 kPa, 1,000 kPa). Grooved and planar substrates were subsequently coated with collagen type I and used to culture the aforementioned cell populations without and with macromolecular crowding (100 μg/ml carrageenan). After 3, 7 and 14 days in culture, cell morphology, viability, metabolic activity, proliferation, protein synthesis and deposition and gene expression analyses were conducted.ResultsNone of the variables assessed affected cell viability, metabolic activity and proliferation. Surface topography was found to be a potent regulator of cell morphology. Macromolecular crowding significantly increased extracellular matrix deposition, albeit in globular manner independently on whether grooved or planar substrates were used, possibly due to the low dimensionality of the grooves. Gene expression analysis made apparent that the 130 kPa and the 1,000 kPa grooved substrates under macromolecular crowding conditions maintained human tenocyte phenotype and directed human bone marrow mesenchymal stromal cells towards tendon-like lineage, respectively. None of the conditions assessed dramatically affected human skin fibroblast fate.ConclusionsCollectively, our data indicate that the physicochemical in vitro microenvironment modulators assessed herein are capable of maintaining human tenocyte phenotype and differentiating human bone marrow mesenchymal stromal cells towards tenogenic lineage, but not in trans-differentiating human skin fibroblasts.