bluuebunny/crossref_metadata_2025_split huggingface dataset:
The neuropeptide Galanin is required for homeostatic rebound sleep following increased neuronal activity
Authors: Reichert Sabine , Pavón Arocas Oriol , Rihel Jason 
Published: November 2018

AbstractSleep pressure homeostatically increases during wake and dissipates during sleep, but the molecular signals and neuronal substrates that measure homeostatic sleep pressure remain poorly understood. We present a pharmacological assay in larval zebrafish that generates acute, short-term increases in wakefulness followed by sustained rebound sleep after washout. The intensity of global neuronal activity during drug-induced wakefulness predicted the amount of subsequent rebound sleep. Whole brain mapping with the neuronal activity marker phosphorylated extracellular signal–regulated kinase (pERK) identified preoptic Galanin-expressing neurons as selectively active during rebound sleep, and the relative induction of galanin transcripts was predictive of total rebound sleep time. Galanin is required for sleep homeostasis, as galanin mutants almost completely lacked rebound sleep following both pharmacologically induced neuronal activity and physical sleep deprivation. These results suggest that Galanin plays a key role in responding to sleep pressure signals derived from neuronal activity and functions as an output arm of the vertebrate sleep homeostat. (word count: 158).
IFT25 is required for the construction of the trypanosome flagellum
Authors: Huet Diego , Blisnick Thierry , Perrot Sylvie , Bastin Philippe 
Published: November 2018

AbstractIntraflagellar transport (IFT), the movement of protein complexes responsible for the assembly of cilia and flagella, is remarkably well conserved from protists to humans. However, two IFT components (IFT25 and IFT27) are missing from multiple unrelated eukaryotic species. In mouse, IFT25 and IFT27 are not required for assembly of several cilia with the noticeable exception of the flagellum of spermatozoa. Here we show that theTrypanosoma bruceiIFT25 protein is a proper component of the IFT-B complex and displays typical IFT trafficking. Using bimolecular fluorescence complementation assays, we reveal that IFT25 and IFT27 interact within the flagellum in live cells during the IFT transport process. IFT25-depleted cells construct tiny disorganised flagella that accumulate IFT-B proteins (with the exception of IFT27, the binding partner of IFT25) but not IFT-A proteins. This phenotype is comparable to the one following depletion of IFT27 and shows that IFT25/IFT27 constitute a specific module requested for proper IFT and flagellum construction in trypanosomes. We discuss the possible reasons why IFT25/IFT27 would be required for only some types of cilia.
Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells
Authors: Chadchankar Jayashree , Korboukh Victoria , Doig Peter , Jacobsen Steve J. , Brandon Nicholas J. , Moss Stephen J. , Wang Qi 
Published: November 2018

AbstractUSP14 is a cysteine-protease deubiquitinase associated with the proteasome and plays important catalytic and allosteric roles in proteasomal degradation. USP14 inhibition has been considered a therapeutic strategy for accelerating degradation of aggregation-prone proteins in neurodegenerative diseases and for inhibiting proteasome function to induce apoptotic cell death in cancers. Here we studied the effects of USP14 inhibition in mammalian cells using small molecule inhibitors and an inactive USP14 mutant C114A. Neither the inhibitors nor USP14 C114A changed the level of TDP-43, tau or α-synuclein in HEK293T cells. However, USP14 C114A led to an accumulation of ubiquitinated proteins, which were isolated by ubiquitin immunoprecipitation and identified by mass spectrometry. Among these proteins we confirmed that ubiquitinated β-catenin was accumulated in the cells expressing USP14 C114A with biochemistry and molecular biology experiments. The proteasome binding of USP14 C114A is required for its effect on ubiquitinated proteins. UCHL5 is the other cysteine-protease deubiquitinase associated with the proteasome. Interestingly, the inactive mutant of UCHL5 C88A also caused an accumulation of ubiquitinated proteins in HEK293T cells but did not affect β-catenin. Using ubiquitin immunoprecipitation and mass spectrometry, we identified the accumulated ubiquitinated proteins in UCHL5 C88A expressing cells which are mostly distinct from those accumulated in USP14 C114A expressing cells. Among the identified proteins are well established proteasome substrates and proteasome subunits. Together our data suggest that USP14 and UCHL5 can deubiquitinate distinct substrates at the proteasome and regulate the ubiquitination of the proteasome itself which is tightly linked to its function.
Integrative analysis from the epigenome through translation exposes patterns of dominant nuclear regulation during transient stress
Authors: Lee Travis A , Bailey-Serres Julia 
Published: November 2018

AbstractGene regulation is modulated from chromatin to translation. To better understand the integration of nuclear and cytoplasmic gene regulatory dynamics, we performed a multi-omic survey of the epigenome through the translatome of the response ofArabidopsisseedlings to hypoxia and reoxygenation. This included eight assays of chromatin (histones, accessibility, RNAPII and transcription factor binding) and three assays of RNA (nuclear, polyadenylated, and ribosome-associated). Dynamic patterns of nuclear regulation distinguished stress-induced and growth-associated mRNAs. The rapid upregulation of hypoxia-responsive gene transcripts and their preferential translation was accompanied by increased chromatin accessibility, RNAPII engagement and reduced Histone 2A.Z association. The more progressive upregulation of heat stress gene transcripts was characterized by early engagement of RNAPII and elevation of nuclear over polyadenylated RNA. Promoters of the rapidly versus progressively upregulated gene cohorts were enriched forcis-elements of ethylene-responsive and heat shock factor transcription factor families, respectively. By contrast, genes associated with growth including ribosomal proteins underwent distinct histone modifications, yet retained RNAPII engagement and accumulated nuclear transcripts during the stress. Upon reaeration, many of the progressively upregulated and growth-associated gene transcripts were mobilized to ribosomes. Thus, multi-level nuclear regulation distinguishes transcript synthesis, accumulation and translation in response to a transient stress.
Dynamical Task Switching in Cellular Computers
Authors: Goñi-Moreno Angel , Cruz Fernando de la , Rodríguez-Patón Alfonso , Amos Martyn 
Published: November 2018

AbstractWe present a scheme for implementing a version of task switching in engineered bacteria, based on the manipulation of plasmid copy numbers. Our method allows for the embeddingof multiple computations in a cellular population, whilst minimising resource usage inefficiency. We describe the results of computational simulations of our model, and discuss the potential for future work in this area.
6-Phosphogluconate Dehydrogenase Links Cytosolic Carbohydrate Metabolism to Protein Secretion
Authors: Li Haoxin , Ericsson Maria , Rabasha Bokang , Budnik Bogdan , Wagner Bridget , Garraway Levi A. , Schreiber Stuart L. 
Published: November 2018

SUMMARYThe proteinaceous extracellular matrix (ECM) is vital for cancer cell survival, proliferation, migration, and differentiation. However, little is known regarding metabolic pathways required in the ECM secretion process. By using an unbiased computational approach, we searched for enzymes whose suppression may lead to disruptions in protein secretion. Here, we show that 6-phosphogluconate dehydrogenase (PGD), a cytosolic enzyme involved in carbohydrate metabolism, is required for endoplasmic reticulum (ER) structural integrity and protein secretion. Chemical inhibition or genetic suppression of its activity led to cell stress accompanied by significantly expanded ER volume and can be rescued by compensating glutathione supplies. Our results also suggest that this characteristic ER-dilation phenotype may be a general marker indicating increased ECM protein congestion inside cells and decreased secretion. Thus, PGD exemplifies a nexus of cytosolic carbohydrate metabolism and protein secretion.
Multi-objective ant lion optimization algorithm to solve large-scale multi-objective optimal reactive power dispatch problem
Authors: Mouassa Souhil , Bouktir Tarek 
Published: November 2018

Purpose
In the vast majority of published papers, the optimal reactive power dispatch (ORPD) problem is dealt as a single-objective optimization; however, optimization with a single objective is insufficient to achieve better operation performance of power systems. Multi-objective ORPD (MOORPD) aims to minimize simultaneously either the active power losses and voltage stability index, or the active power losses and the voltage deviation. The purpose of this paper is to propose multi-objective ant lion optimization (MOALO) algorithm to solve multi-objective ORPD problem considering large-scale power system in an effort to achieve a good performance with stable and secure operation of electric power systems.

Design/methodology/approach
A MOALO algorithm is presented and applied to solve the MOORPD problem. Fuzzy set theory was implemented to identify the best compromise solution from the set of the non-dominated solutions. A comparison with enhanced version of multi-objective particle swarm optimization (MOEPSO) algorithm and original (MOPSO) algorithm confirms the solutions. An in-depth analysis on the findings was conducted and the feasibility of solutions were fully verified and discussed.

Findings
Three test systems – the IEEE 30-bus, IEEE 57-bus and large-scale IEEE 300-bus – were used to examine the efficiency of the proposed algorithm. The findings obtained amply confirmed the superiority of the proposed approach over the multi-objective enhanced PSO and basic version of MOPSO. In addition to that, the algorithm is benefitted from good distributions of the non-dominated solutions and also guarantees the feasibility of solutions.

Originality/value
The proposed algorithm is applied to solve three versions of ORPD problem, active power losses, voltage deviation and voltage stability index, considering large -scale power system IEEE 300 bus.
Organizational justice and turnover intentions: probing the Pakistani print media sector
Authors: Hussain Muzammil , Khan Mohammad Saud 
Published: November 2018

PurposeTo survive in this competitive era of modern business environment, organizations have to constantly develop, adapt and react to new challenges. Therefore, it is critical for organizations to create a sense of justice and involve their employees in business activities; thereby achieving the organization’s strategic goals. The purpose of this paper is to investigate the impact of organizational justice (OJ) on job turnover behavior of employees within the print media sector of Pakistan.Design/methodology/approachThe study employs a quantitative methodology. It uses a sample of 402 employees from the newspaper industry to test hypotheses using regression analysis.FindingsResults indicate that perceived distributive justice, procedural justice and informational justice have a negative relationship with turnover intentions of employees (as hypothesized) whereas; interpersonal justice did not result in a significant relationship.Originality/valueThe present study is one of the first within a Pakistani context in print media sector, aiming to examine the relationship of OJ (addressing all of its dimensions) and “intentions to leave the organization.”
Predicting future AI failures from historic examples
Authors: Yampolskiy Roman V. 
Published: November 2018

Purpose
The purpose of this paper is to explain to readers how intelligent systems can fail and how artificial intelligence (AI) safety is different from cybersecurity. The goal of cybersecurity is to reduce the number of successful attacks on the system; the goal of AI Safety is to make sure zero attacks succeed in bypassing the safety mechanisms. Unfortunately, such a level of performance is unachievable. Every security system will eventually fail; there is no such thing as a 100 per cent secure system.

Design/methodology/approach
AI Safety can be improved based on ideas developed by cybersecurity experts. For narrow AI Safety, failures are at the same, moderate level of criticality as in cybersecurity; however, for general AI, failures have a fundamentally different impact. A single failure of a superintelligent system may cause a catastrophic event without a chance for recovery.

Findings
In this paper, the authors present and analyze reported failures of artificially intelligent systems and extrapolate our analysis to future AIs. The authors suggest that both the frequency and the seriousness of future AI failures will steadily increase.

Originality/value
This is a first attempt to assemble a public data set of AI failures and is extremely valuable to AI Safety researchers.
Teaching social economics
Authors: Farias Christine , Balardini Fabian 
Published: November 2018

Purpose
Teaching complex economic theories can be made relevant through everyday life experiences and current economic, social, and environmental crises can be used as vehicles for student learning. The purpose of this paper is to help students understand that the economy should be seen as a social system that evolves over time driven by conflictive and contradictory forces and enable them to develop the critical thinking skills needed to make better choices for a more equitable and sustainable future.

Design/methodology/approach
The paper reviews the historical role played by neoliberalism on education in general and on the teaching of economics in particular. A historical/critical/action-learning approach discusses five pedagogical teaching methods that have been implemented in undergraduate economics courses and demonstrates how teaching social economics can be made possible by bringing the real world into the classroom and taking the classroom into the real world.

Findings
There is an urgent need to rethink the teaching of economics and the economics curriculum from one that stresses self-interest, profit maximization and cost minimization, to one that stresses cooperation, collaboration, fairness, and ethical values rather than economic value, as ways of satisfying society’s needs and addressing systemic issues of inequality, power and greed. Bringing teachers and students together in collaborative learning environments, thereby learning from the mistakes of the past and minimizing the impacts of the present so that future generations can also participate, is the much-needed change in how social economics can be taught post-financial crisis.

Originality/value
This paper is a response to the special issue on the theme teaching social economics during the global financial crisis. The authors have provided insights into their teaching pedgagogy in the context of this topic.
Viscous shear effect in non-Newtonian lubrication of finite porous elastic bearings
Authors: Sakim Anas , Nabhani Mohamed , El Khlifi Mohamed 
Published: November 2018

Purpose
The purpose of this study is to investigate the viscous shear effect on finite porous elastic journal bearings lubricated with non-Newtonian couple stress fluid.

Design/methodology/approach
Based on Stokes micro-continuum mechanics, the modified Reynolds equation including bearing deformation was derived. The porous flow was modeled by the complete Darcy–Brinkman equation. To show the viscous shear effects, bearing characteristics including load capacity and friction factor are compared to those obtained from Darcy model with Beavers–Joseph slip conditions (slip flow model [SFM]) by developing a computer program and discussed for different couple stress values, permeabilities and elastic deformation parameters.

Findings
It is found that the viscous shearing forces effects of the Brinkman model increase the load capacity and friction factor compared to those derived using SFM. Moreover, the couple stresses increase the load capacity while decreasing the friction factor for both models.

Originality/value
This study introduces for the first time the viscous effect on finite porous elastic journal bearings lubricated with couple stress fluid.
Coping with uncertainty in the internationalisation strategy
Authors: Magnani Giovanna , Zucchella Antonella 
Published: November 2018

PurposeThe purpose of this paper is to explore uncertainty-coping strategic actions in the internationalisation strategy of entrepreneurial ventures, encompassing born globals/international new ventures, enduring established internationalisers, old born globals, born-again globals and micro-multinationals.Design/methodology/approachThe authors developed a qualitative exploratory study applying a grounded theory approach to ten entrepreneurial firms to investigate the strategies they adopted to cope with Knightian uncertainty in international markets.FindingsThe global niche strategy emerged as a successful path to deal with uncertainty in smaller firms’ internationalisation. The authors uncover the components of this strategy, namely the creation of markets, the focus on global clients and the control of technology.Originality/valueThe contribution of this paper consists in exploring how entrepreneurial firms cope with uncertainty through a global niche strategy and in outlining its main components. The authors develop a model of smaller entrepreneurial firms’ international strategising under this perspective. The research thus links together international marketing and strategy with (international) entrepreneurship studies.
Examining the role of perceived investment in employees’ development on work-engagement of liquid knowledge workers
Authors: Jha Jatinder Kumar , Pandey Jatin , Varkkey Biju 
Published: November 2018

PurposeThis paper aims to examine the relationship between perceived investments in employees’ development (PIED) on work engagement and the moderating effects of psychological capital on this relationship for liquid knowledge workers, employed in the Indian cutting and polishing of diamond industry (CPD).Design/methodology/approachA questionnaire composed of established scales was administered to 134 liquid knowledge workers. Having established convergent and discriminant validity using structural equation modelling, the model was further analysed using the Process macro to check for direct and moderating effects.FindingsThe research findings suggest that the perceived investment in employee development and psychological contract enhancement (relational and transactional) made by CPD units for liquid knowledge workers positively influenced their work engagement level. The study also finds that relational contract (not transactional contract) positively moderates the relationship between perceived investment in employee development and work engagement.Research limitations/implicationsThis is a cross-sectional single source study; future studies could look at longitudinal and multisource perspective.Practical implicationsThe study presents a “star matrix of engagement” that guides the application of the two strategies of perceived employee development and psychological contract enhancement for liquid knowledge workers. This has implications for design and implementation of human resource management practices and policies for employee management.Originality/valueThe study makes significant contributions to existing literature on antecedents of work engagement of liquid knowledge workers by examining the direct and moderating influences.
Sustaining innovation and change in government sector organizations
Authors: Limba Rekson S. , Hutahayan Benny , Solimun Solimun , Fernandes Adji 
Published: November 2018

PurposeThe purpose of this paper is to provide an examination of the processes involved in institutionalizing change in government organizations. Following the lens of organizational learning, the authors seek to delineate strategies for fashioning long-lasting change in government settings.Design/methodology/approachDrawing from a large body of literature in organizational studies and public administration, this paper presents arguments and propositions about how organizational politics can enhance learning in government. The paper integrates the concept of power into the organizational learning framework, taking the 4I learning framework as a point of departure.FindingsThe paper formulates insights into how specific power mechanisms can be combined to facilitate the development of flows of learning in government organizations that are in change management process, and concludes that government settings represent a good example of organizations where pathological patterns of learning will be preeminent.Practical implicationsThe paper provides an extension to discussions of the political dynamics of organizational learning. In doing so, the paper highlights the importance of the often over-looked feedback process in organizational learning. It also brings to the fore the importance of strategic leadership in change management processes in government sector organizations.Originality/valueThe paper serves to add impetus to change management discourse in the public sector by presenting a framework for institutionalizing learning in government settings. The paper also sheds light on how different forms of power may be balanced at each stage of the learning process in order to enhance institutionalization.
Export knowledge
Authors: Di Fatta Davide , Gera Navneet , Tyagi Lokinder Kumar , Grisold Thomas 
Published: November 2018

Purpose
This paper aims to study the export knowledge to be the determinant of export strategy, export commitment and export performance in carpet Industry.

Design/methodology/approach
Using a qualitative–quantitative approach, the unit of analysis is the individual export venturing firm in India. More in detail, a qualitative analysis was conducted through a focus group interview to explore the challenges of carpet exports. A quantitative analysis was performed using confirmatory factor analysis (CFA) and, because of covariate nature of the proposed research model, structural equation modeling to evaluate the research hypotheses.

Findings
The results emphasized that Indian carpet exports face major challenges, namely, issues of raw material that is wool, shortage of labor for weaving carpets and a lack of organization which has a negative impact on productivity and quality. Furthermore, this study shows that export knowledge directly influences the export strategy, export commitment and export performance.

Originality/value
Building on the results, this paper suggests corrective measures, as well as required knowledge, to formulate a strategy and boost the export performance of the carpet sector.