bluuebunny/crossref_metadata_2025_split huggingface dataset:
Desarrollo y validación de un método LC-HESI MS/MS para detectar y cuantificar N-nitrosaminas en Valsartán
Authors: Reina-Velasco Carlos , Brito-Dumancela Carlos 
Published: November 2024

En el presente estudio, se desarrolló y validó un método LC-HESI-MS/MS robusto, eficiente y sensible para detectar y cuantificar simultáneamente 5 N-nitrosaminas (N-nitrosodietilamina, N-nitrosoetilisopropilamina, N-nitrosodiisopropilamina, N-nitrosodibutilamina y ácido N-nitrosometilaminobutírico) en Valsartán, un ingrediente activo de la familia de los Sartanes. La separación cromatográfica se logró con una columna Accuore C18 (2,6 µm, 150 x 3 mm) con elución en gradiente utilizando ácido fórmico al 0,1% en agua (fase móvil A) y metanol (fase móvil B). Los límites de detección y cuantificación de las N-nitrosaminas oscilaron entre 0,0337 a 0,0628 ng/mL y 0,1022 a 0,2370 ng/mL, respectivamente, demostrando la alta sensibilidad y capacidad cuantitativa del método. La recuperación de las N-nitrosaminas se situó entre 70% y 130%, y la repetibilidad y precisión intermedia tuvieron coeficientes de variación menores al 10%, evaluados en el producto Pertena Complete el mismo que contiene 3 principios activos farmaceuticos (Amlodipino, Hidroclorotiazida y Valsartán). Las curvas de calibración se construyeron en un rango de 1,0 a 15,0 ng/mL, obteniendo coeficientes de determinación (R2) mayores a 0,995. La estadística jugó un papel crucial en este estudio, permitiendo determinar la precisión, exactitud y fiabilidad del método mediante análisis de varianza, estudios de linealidad y la construcción de intervalos de confianza. Este estudio confirma la seguridad del producto Pertena Complete, fabricado por Prophar SA, y destaca la efectividad del método para un análisis preciso y confiable de N-nitrosaminas en el ingrediente farmacéutico activo Valsartán.
Evaluación de las Estrategias de Aprendizaje de los Estudiantes de una Institución de Educación Superior Tecnológica del Estado de Veracruz
Authors: Chiunti-González Nayeli , Quintero-Fuentes Martha , González-Pérez Manuel , Castillo-Cedillo Pedro 
Published: November 2024

Las estrategias de aprendizaje son fundamentales para la adquisición del conocimiento. La presente investigación tiene como objetivo evaluar las estrategias de aprendizaje de los estudiantes de una Institución de Educación Superior Tecnológica del Estado de Veracruz. El presente estudio es no experimental, con un alcance descriptivo y comparativo. Los participantes son 169 estudiantes de una Institución Superior Tecnológica, el cuestionario utilizado fue CEVEAPEU de Gargallo. Las estrategias afectivas, de apoyo y control presentaron un nivel alto excepto en los componentes afectivos donde mostraron un nivel medio. Por su parte, las estrategias cognitivas presentaron un nivel alto. Las estrategias afectivas de apoyo y control mostraron diferencias entre el cuarto y el décimo cuatrimestre, excepto con las estrategias motivacionales, donde no se encontraron diferencias. Por su parte, las estrategias cognitivas mostraron diferencias entre el cuarto y el décimo cuatrimestre, excepto en las estrategias de procesamiento y uso de la información donde no se encontraron diferencias. Conclusión: El nivel de los estudiantes con respecto a las estrategias de aprendizaje es alto, sin embargo, se debe generar estrategias para mejorar los componentes afectivos que permitan un mejor rendimiento, por otro lado, se comprobó que, a mayor cuatrimestre, mejor gestión de las estrategias de aprendizaje.
La sentencia no. 200-12-jh/21 de la corte constitucional del ecuador y su incidencia en la recuperación de menores de edad por retención indebida.
Authors: Remache-Suarez Juan , Alvarado-Ajila Lissette , García-Segarra Holger 
Published: November 2024

La investigación se centra en la incidencia de la Sentencia No. 200-12-JH/21 de la Corte Constitucional del Ecuador en los derechos del progenitor custodio en casos de recuperación de menores retenidos indebidamente, ello se fundamenta en la creciente preocupación por la vulneración de derechos en estos procesos judiciales, específicamente en la parroquia Tumbaco, cantón Quito. El principal objetivo de esta investigación es analizar cómo la sentencia No. 200-12-JH/21 afecta los derechos del custodio en estos casos de recuperación de menores retenidos indebidamente. Para alcanzar este propósito, se plantean varios objetivos específicos que guiarán la investigación los cuales son identificar los derechos del custodio según la sentencia, evaluar su aplicación en la práctica, y proponer recomendaciones para mejorar la protección de estos derechos. Se utiliza un enfoque mixto, con técnicas que incluyen revisión bibliográfica, observación directa en la Unidad Judicial de Familia de Tumbaco, revisión de casos judiciales y triangulación de métodos para validar los resultados. Se emplean los métodos histórico, exegético, inductivo y lógico para un análisis comprensivo. La investigación revela que existe una correlación entre el uso inadecuado de la sentencia y la incertidumbre y riesgos de decisiones arbitrarias, afectando negativamente la protección de los derechos del progenitor custodio y el interés superior del niño, siendo necesario mejorar su aplicación mediante recomendaciones específicas que fortalezcan la defensa y protección de los derechos del custodio y del menor en los procesos judiciales.
Reseña de "Empowering teachers across Europe to deal with social and emotional diversity-related challenges"
Authors: Gomez Baya Diego 
Published: November 2024

Kozina, A. (Ed.) (2024). Empowering teachers across Europe to deal with social and emotional diversity-related challenges. Volume I: Experimentation perspectives. Waxmann. ISBN 978-3-8309-4859-9

Reseña realizada por Diego Gómez Baya, Profesor Titular del Departamento de Psicología Social, Evolutiva y de la Educación, Universidad de Huelva, España
Editorial: Artes Visuais, imaginários de esquerda e capitalismo na América Latina
Authors: Bugnone Ana , Fetter Bruna , Cabral de Lira Jordao Fabricia , Cavalcanti Raiza , Fabris Yasmin , De la rosa Natália 
Published: November 2024

Editorial
Artes Visuais, imaginários de esquerda e capitalismo na América Latina

O imaginário revolucionário cumpre um papel importante na relação entre arte e política. Inclusive, foi a violência revolucionária e o laicismo iconoclasta da Revolução Francesa que produziu a noção moderna de arte (Groys, 2021). Na América Latina, a partir da década de 1960, os imaginários atrelados às revoluções francesa e soviética foram atualizados e reformulados desde as experiências históricas coloniais, o triunfo da revolução cubana (1953-1959) e os sucessivos golpes de Estado que estabeleceram governos ditatoriais no Brasil (1964), Bolívia (1964), Argentina (1966 e depois em 1976), Chile (1973) e Uruguai (1973). Para Ana Longoni (2014), vanguarda e revolução funcionaram como “ideias-forças” na arte desse período. A associação entre revolução e vanguarda, sobretudo nos primeiros anos das ditaduras militares do Cone Sul, redefiniu o papel da arte e dos artistas.
O léxico político-ideológico assume o protagonismo nos enunciados, nas produções poéticas de artistas e nas formulações críticas e teóricas do continente, agregando novas camadas de significação para o gesto artístico, agora convertido em ação revolucionária. No Brasil, o ideário ético-político-estético vigente no período se evidenciou em conceituações como “geração tranca-ruas” (Bittencourt, 1970) e “arte de guerrilha” (Morais, 1970), empregadas por críticos e artistas na tentativa de dar unidade e, ao mesmo tempo, distinguir uma vasta e
diversificada produção que foi difundida em mostras como Opinião 65 (1965), Arte no Aterro (1968), Do corpo à Terra (1970) e Domingos de criação (1971).
No entanto, a crença na possibilidade de produzir uma forma artística revolucionária, a partir do paradigma da intervenção da experiência estética na realidade social, subjacente à relação arte e política dentro do espectro socialista, rapidamente encontrou seu limite. A partir daí, diversos artistas entraram em um impasse: como constituir um imaginário político nas artes visuais que não estivesse atrelado a projetos políticos-revolucionários instrumentalizadores do estético, e que também valorizavam o uso da violência, do matar e do morrer, como opção legítima da atividade política?
Apigeninidin chloride disrupts Toxoplasma gondii Mitochondrial membrane potential and induce reactive oxygen species and metabolites production
Authors: Moon Miya Janelle , Kamasah Japhet Senyo , Sharma Homa Nath , Robertson Boakai K. , Abugri Daniel A. 
Published: November 2024

IntroductionApigeninidin chloride (APi) is a form of 3-deoxyanthrocyanidins (3-DAs) abundantly produced by the red Sorghum bicolor plant. It has been previously reported to be effective against Toxoplasma gondii (T. gondii) tachyzoites grown in vitro with less cytotoxic effect. However, its possible mechanism(s) of action has not been elucidated. Biochemically, we discovered that APi induced high reactive oxygen species (ROS) and mitochondria superoxide (MitoSOX) productions in tachyzoites, leading to mitochondrial membrane potential (MMP) disruption in vitro.MethodsTo confirm our biochemical results at the molecular level, we performed a liquid chromatography-mass spectrometry (LC-MS) analysis on APi-treated parasites to assess any metabolite and lipid alterations often associated with high ROS/MitoSOX production in cells. ResultsNoteworthy is that we detected several important oxidative stress-induced metabolites such as hexanal, aldehydes, methyl undeo10-enoate, butadiynyl phenyl ketone, 16-hydroxyhexadecanoic acid (16-OH, 16:0), 2-hydroxytricosanoic acid (C23:0; O), 3-oxodecanosanoic acid (C22:1; O), 2-hydroxypropylsterate, and furan fatty acids F6 (19FU-FA). DiscussionThese metabolites are associated with lipid, protein, and nucleic acid disruptions. Using atovaquone (Atov) as a control, we observed that it disrupted intracellular tachyzoites’ mitochondrial membrane potential, increased ROS and MitoSOX production, and altered metabolite and lipid production similar to what was observed with our experimental compound APi. Overall, our results indicated that APi targets T. gondii tachyzoite growth through inducing oxidative stress, mitochondrial dysfunction, and eventually parasite death.
Gesture alignment in teacher–student interaction: a study concerning office hour consultations using English as the lingua franca
Authors: Opazo Paloma , Cienki Alan , Oben Bert , Brône Geert 
Published: November 2024

IntroductionIn this study, we explore the presence of gesture alignment in office hour consultations, a form of academic talk characterized by private face-to-face dialogues between a lecturer and a university student. Unlike classroom interactions, the topic of these consultations is initiated by the student. Our objectives were to describe the patterns of gesture alignment in these educational settings, to determine the direction of the copied behavior (i.e., who copies whom?), and to understand the temporal structuring of these instances.MethodsWe analyzed 12 office hour consultations, involving Spanish undergraduate students and lecturers from universities in England, Ireland, and Sweden. All the conversations were held in English. The annotation considered three domains: the timing of matching gestures (i.e., if the aligned gestures appeared in a Simultaneous, Consecutive, or Later manner), the form features of the aligned gestures (hand shape, movement, and orientation), and the function of the gestures (representational, deictic, or pragmatic).ResultsOur results show that although there are important differences between dyads, there were four general findings. First, aligned gestures mostly took place in a Consecutive manner. Second, gesture alignment is shown to achieve shared understanding between interactants, but this can be manifested in different ways: from the active negotiation of meaning to the signaling of agreement. Third, paired gestures become useful in educational contexts where the teachers and students include native and non-native speakers, as they contribute to disambiguating meaning. Fourth, many cases of matching gestures happen due to the presence of recurrent gestural forms.DiscussionOverall, our results are in line with previous evidence that has highlighted the role of gesture alignment in grounding processes, related to the achievement of mutual agreement between participants. Matching gestures are a helpful resource during office hour consultations—a form of academic talk where content is being explained and negotiated.
Association of metabolic score for insulin resistance with progression or regression of prediabetes: evidence from a multicenter Chinese medical examination cohort study
Authors: Sheng Guotai , Kuang Maobin , Yang Ruijuan , Zou Yang 
Published: November 2024

ObjectiveFew studies have evaluated the changes in blood glucose status in individuals with prediabetes, and this study aimed to analyze the association between metabolic score for insulin resistance (MetS-IR) and the progression or regression of prediabetes.MethodsThis retrospective cohort study used research data from medical examination institutions under the Rich Healthcare Group in 32 regions across 11 cities in China. Progression of prediabetes to diabetes and regression to normal fasting glucose (NFG) were defined based on glycemic changes during follow-up. The association between MetS-IR and the progression or regression of prediabetes was analyzed using multivariate Cox regression, restricted cubic splines, and piecewise regression models.ResultsData from 15,421 prediabetic subjects were analyzed. Over an average follow-up of 2.96 years, 6,481 individuals (42.03%) returned to NFG, and 2,424 (15.72%) progressed to diabetes. After controlling for confounding factors, an increase in MetS-IR was observed to increase the risk of diabetes onset in the prediabetic population, whereas a decrease in MetS-IR had a protective effect for returning to NFG. Additionally, a nonlinear relationship between MetS-IR and prediabetes regression was observed, with 37.22 identified as the inflection point; prediabetes regression rates were significantly higher before this point and markedly decreased after it.ConclusionFor individuals with prediabetes, an increase in MetS-IR may lead to an increased risk of diabetes; conversely, a decrease in MetS-IR enhances the protective effect for returning to NFG and keeping MetS-IR below 37.22 is significant for the regression of prediabetes.
A U-shaped relationship between the atherogenic index of plasma and repeated target vessel revascularization in patients undergoing percutaneous coronary intervention: a retrospective study
Authors: Huang Xingjie , Wen Song , Huang Zehan , Qin Guishi , Zhou Hanli , Xia Zhonghua 
Published: November 2024

BackgroundAtherogenic index of plasma (AIP) has been recognized as a novel and practical marker for the assessment of cardiometabolic risk, but the relevance of AIP as a prognostic biomarker in coronary artery disease (CAD) remains debated. This study investigated the association between AIP and major adverse cardiac and cerebrovascular events (MACCEs) in CAD patients receiving percutaneous coronary intervention (PCI) with drug-eluting stents (DES).MethodsA total of 2,250 patients undergoing PCI with DES were included in this retrospective cohort study. The primary endpoint was MACCEs, encompassing acute myocardial infarction, repeat target vessel revascularization (TVR), stroke, and all-cause mortality. Logistic regression models were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs). Restricted cubic splines were applied to explore the dose–response associations. And subgroup analysis was conducted to evaluate potential relationship between AIP and MACCEs across different subgroups.ResultsDuring a medium follow-up of 29.8 (25.6–34.0) months, 106 (4.7%) patients experienced TVR. After adjusting for confounders, AIP (per 1 SD increase) was positively associated with TVR (odds ratio [OR] = 1.26, 95% confidence interval [CI] = 1.01–1.58, P = 0.042). In females, there was a significant association (OR = 2.33, 95% CI = 1.40–3.98, P = 0.002), but no significant association was observed in males. There was an interaction between AIP and gender (P = 0.017). Restricted cubic spline analysis depicted a U-shaped relationship between AIP and TVR (Pnonlinear = 0.016), with an elevated risk evident from an AIP of 0.20.ConclusionAIP showed a U-shaped relationship with TVR in PCI patients with DES, particularly pronounced among females. We suggested that the AIP should be used as a plasma marker of key interest for preventing TVR after DES implantation in patients with CAD.
Dysregulated CD38 expression on T cells was associated with rapidly progressive interstitial lung disease in anti-melanoma differentiation-associated gene 5 positive dermatomyositis
Authors: Guo Yixue , Liu Hongjiang , Chen Bo , Zhang Keyi , Meng Liye , Yan Lin , Niu Qian , Zhang Junlong , Yin Geng , Li Yi 
Published: November 2024

BackgroundAnti-melanoma differentiation-associated gene 5 positive dermatomyositis (MDA5+ DM) is a life-threatening disease due to rapidly progressive interstitial lung disease (RP-ILD). We aimed to investigate the expression profile of T cell subsets in MDA5+ DM patients, seeking for possible disease-causing T cell subsets and potential biomarkers to distinguish ILD, especially RP-ILD patients.MethodsPeripheral blood T cell subpopulations were immunophenotyped in 24 MDA5+ DM patients and 21 healthy controls (HCs) by flow cytometry. The proportion of T cell subsets and clinical characteristics were analyzed. The quantitative determination of cytokines in the plasma was measured by using a microsphere-based immunofluorescence assaying kit.ResultsThe proportion of naïve and CD38+ T cells were much higher, whereas the proportion of central memory T cells were lower in MDA5+ DM patients than in HCs. Notably, the proportion of CD38+CD4+ T cells and CD38+CD8+ T cells on T cells in in RP-ILD group were significantly elevated compared to C-ILD, non-ILD group and HCs. Moreover, serum IFN-α levels were significantly increased in MDA5+ DM patients with RP-ILD. Further, the frequencies of CD38+CD4+ T cells and CD38+CD8+ T cells were positively correlated with IFN-α levels. Finally, ROC analysis indicated that CD38+CD4+ T cells and CD38+CD8+ T cells could be potential biomarkers for predicting ILD/RP-ILD in MDA5+ DM patients.ConclusionDysregulated CD38 expression on T cell subsets was associated with lung involvement, especially RP-ILD in MDA5+ DM patients. CD38+ T cell subsets could be used as potential biomarkers for predicting ILD/RP-ILD in MDA5+ DM patients.
Immunogenicity of dupilumab in adult and pediatric patients with atopic dermatitis
Authors: Kamal Mohamed A. , Kosloski Matthew P. , Lai Ching-Ha , Partridge Michael A. , Rajadhyaksha Manoj , Kanamaluru Vanaja , Bansal Ashish , Shabbir Arsalan , Shumel Brad , Ardeleanu Marius , Richards Susan M. , Yan Hong , Xu Christine R. , Rodríguez-Marco Ainara , Xiao Jing , Khokhar Faisal A. , Gherardi Guy , Babilonia Elisa , Maloney Jennifer , Mortensen Eric , Akinlade Bolanle , Braunstein Ned , Stahl Neil , Torri Albert , Davis John D. , DiCioccio A. Thomas 
Published: November 2024

BackgroundDevelopment of anti-drug antibodies (ADAs) and neutralizing antibodies (NAbs) to monoclonal antibodies may adversely impact pharmacokinetics, efficacy, and/or safety.ObjectiveTo describe incidence, titer, and persistence of dupilumab ADAs and NAbs, and their effects on pharmacokinetics, efficacy, and safety in patients with atopic dermatitis (AD).MethodsThis analysis included seven phase 3 randomized, placebo-controlled (N=2,992) and two long-term open-label extension (N=2,287) trials of subcutaneous dupilumab in adults and pediatric patients with moderate-to-severe AD. ADA, NAb, and dupilumab concentration in serum were assessed using validated immunoassays. ADA impacts on efficacy (EASI) and safety were assessed.ResultsTreatment-emergent ADAs were observed in up to 8.6% (aged ≥18 years), 16.0% (12-17 years), 5.3% (6-11 years), and 2.0% (6 months to 5 years) dupilumab-treated patients. Among dupilumab-treated patients, ≤3.7% had persistent responses, &lt;1% had high titers (≥10,000), and ≤5.1% were NAb-positive. NAbs were more common in patients with moderate- and high-titer ADA responses. High-titer ADAs, while infrequent, were the variable most associated with lower dupilumab concentrations in serum and loss of efficacy, independent of NAb status. Efficacy was generally similar in ADA-positive and -negative patients. For most patients with high- or moderate-titer ADAs, titers decreased and efficacy improved over time with continued dupilumab treatment. ADA-positive and -negative patients had similar incidences of treatment-emergent and serious treatment-emergent adverse events. One patient with high-titer ADAs developed serum sickness.ConclusionIn patients with AD, ADAs and NAbs had minimal impact on dupilumab concentration, efficacy, and safety, except for high-titer ADAs in a small number of patients.Clinical trial registrationClinicalTrials.gov, identifiers (NCT02277743, NCT02277769, NCT02260986, NCT02395133, NCT01949311, NCT03054428, NCT03345914, NCT02612454, and NCT03346434).
Comparison of adjuvant treatment regimens for high-risk hepatocellular carcinoma: a Bayesian network meta analysis and systematic review
Authors: Li Jiahao , Liu Yingnan , Qiu Yuqi , Qu Chao , Li Jiarui 
Published: November 2024

BackgroundThe five-year recurrence rate for patients with hepatocellular carcinoma (HCC) is as high as 70%. Patients with high-risk recurrence factors experience significantly poorer prognosis. Local regional therapies, including transarterial chemoembolisation (TACE), hepatic arterial infusion chemotherapy (HAIC), radiotherapy, and emerging immunotherapy, are commonly used adjuvant treatment options. We conducted an indirect comparison of these adjuvant therapies for such patients.MethodsWe conducted a systematic search in public databases for relevant studies and assessed the efficacy and safety of the corresponding therapies by consolidating disease-free survival (DFS), overall survival (OS), and adverse events (AEs).ResultsA total of eight randomised controlled trials were ultimately included. The Gelman-Rubin plot and kernel density estimation indicate that the stability of the combined model is satisfactory.Conclusionimmunotherapy is not inferior to local regional therapies in delaying tumour recurrence, however, the higher incidence of AEs remains a significant concern. Adjuvant radiotherapy demonstrated superior efficacy in delaying tumour recurrence compared to adjuvant TACE, although further support from phase III clinical trial evidence is required.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42024576316.