bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silencing chitin deacetylase 2 impairs larval–pupal and pupal–adult molts in Leptinotarsa decemlineata
Authors: Wu J.‐J. , Chen Z.‐C. , Wang Y.‐W. , Fu K.‐Y. , Guo W.‐C. , Li G.‐Q. 
Published: July 2018

AbstractInsect chitin deacetylases (CDAs) are carbohydrate esterases that catalyze N‐deacetylation of chitin to generate chitosan, a process essential for chitin organization and compactness during the formation of extracellular chitinous structure. Here we identified two CDA2 splice variants (LdCDA2a and LdCDA2b) in Leptinotarsa decemlineata. Both splices were abundantly expressed in larval foregut, rectum, and epidermis; their levels peaked immediately before ecdysis within each instar. In vivo results revealed that the two isoforms transcriptionally responded, positively and negatively respectively, to 20‐hydroxyecdysone and juvenile hormone signaling pathways. RNA interference (RNAi)‐aided knockdown of the two LdCDA2 variants (hereafter LdCDA2) or LdCDA2b, rather than LdCDA2a, resulted in three negative effects. First, foliage consumption was significantly reduced, larval developing period was lengthened, and larval growth was retarded. Second, chitin contents were reduced, whereas glucose, trehalose, and glycogen contents were increased in the LdCDA2 and LdCDA2b RNAi larvae. Third, approximately 20% of LdCDA2 and LdCDA2b RNAi larvae were trapped within the exuviae and finally died. About 60% of the abnormal pupae died as pharate adults. Around 20% of the RNAi pupae emerged as deformed adults, with small size and wrinkled wings. These adults eventually died within 1 week after molting. Our results reveal that knockdown of CDA2 affects chitin accumulation. Consequently, LdCDA2 may be a potential target for control of L. decemlineata larvae.
Acetyl‐11‐keto‐β‐boswellic acid ameliorates renal interstitial fibrosis via Klotho/TGF‐β/Smad signalling pathway
Authors: Liu Minna , Liu Tianlong , Shang Peijin , Zhang Yikai , Liu Limin , Liu Ting , Sun Shiren 
Published: July 2018

AbstractAcetyl‐11‐keto‐β‐boswellic acid (AKBA), an active triterpenoid compound from the extract of Boswellia serrate, has been reported previously in our group to alleviate fibrosis in vascular remodelling. This study aimed to elucidate the in vivo and in vitro efficacy and mechanism of AKBA in renal interstitial fibrosis. The experimental renal fibrosis was produced in C57BL/6 mice via unilateral ureteral obstruction (UUO). Hypoxia‐induced HK‐2 cells were used to imitate the pathological process of renal fibrosis in vitro. Results showed that the treatment of AKBA significantly alleviated UUO‐induced impairment of renal function and improved the renal fibrosis by decreasing the expression of TGF‐β1, α‐SMA, collagen I and collagen IV in UUO kidneys. In hypoxia‐induced HK‐2 cells, AKBA displayed remarkable cell protective effects and anti‐fibrotic properties by increasing the cell viability, decreasing the lactate dehydrogenase (LDH) release and inhibiting fibrotic factor expression. Moreover, in obstructed kidneys and HK‐2 cells, AKBA markedly down‐regulated the expression of TGFβ‐RI, TGFβ‐RII, phosphorylated‐Smad2/3 (p‐Smad2/3) and Smad4 in a dose‐dependent fashion while up‐regulated the expression of Klotho and Smad7 in the same manner. In addition, the effects of AKBA on the Klotho/TGF‐β/Smad signalling were reversed by transfecting with siRNA‐Klotho in HK‐2 cells. In conclusion, our findings provide evidence that AKBA can effectively protect kidney against interstitial fibrosis, and this renoprotective effect involves the Klotho/TGF‐β/Smad signalling pathway. Therefore, AKBA could be considered as a promising candidate drug for renal interstitial fibrosis.
Morphogenesis and Molecular Phylogeny of a Soil Ciliate Uroleptoides longiseries (Foissner, Agatha and Berger, 2002) Berger 2008 (Ciliophora, Hypotrichia)
Authors: Wang Jingyi , Li Jingbao , Qi Shuyan , Warren Alan , Shao Chen 
Published: July 2018

AbstractMorphogenesis of the hypotrich ciliate Uroleptoides longiseries, isolated from sandy soil beside the Yellow River, Suide, Yulin, Shaanxi Province, China, was investigated using protargol staining. The main events during binary fission are as follows: (1) the long frontoventral row is formed by a single anlage; (2) five frontoventral‐transverse cirral anlagen are formed in primary mode; (3) only the posterior part of the parental adoral zone of the membranelles is renewed; and (4) the oral primordium of the opisthe is formed intrakinetally. This is the first detailed record of all stages of morphogenesis for Uroleptoides. We also provide the first record of the small subunit ribosomal DNA (SSU rDNA) sequences for U. longiseries. Phylogenetic analyses based on the SSU rDNA sequences data show that Uroleptoides longiseries clusters with U. magnigranulosa with moderate to high support which together form a clade with Orthoamphisiella breviseries. These three species share the morphogenetic feature of the long frontoventral row being formed by a single anlage.
Integrating Batteries in the Future Swiss Electricity Supply System: A Consequential Environmental Assessment
Authors: Vandepaer Laurent , Cloutier Julie , Bauer Christian , Amor Ben 
Published: July 2018

SummaryStationary batteries are projected to play a role in the electricity system of Switzerland after 2030. By enabling the integration of surplus production from intermittent renewables, energy storage units displace electricity production from different sources and potentially create environmental benefits. Nevertheless, batteries can also cause substantial environmental impacts during their manufacturing process and through the extraction of raw materials. A prospective consequential life cycle assessment (LCA) of lithium metal polymer and lithium‐ion stationary batteries is undertaken to quantify potential environmental benefits and drawbacks. Projections are integrated into the LCA model: Energy scenarios are used to obtain marginal electricity supply mixes, and projections about the battery performances and the recycling process are sourced from the literature. The roles of key parameters and methodological choices in the results are systematically investigated. The results demonstrate that the displacement of marginal electricity sources determines the environmental implications of using batteries. In the reference scenario representing current policy, the displaced electricity mix is dominated by natural gas combined cycle units. In this scenario, the use of batteries generates environmental benefits in 12 of the 16 impact categories assessed. Nevertheless, there is a significant reduction in achievable environmental benefits when batteries are integrated into the power supply system in a low‐carbon scenario because the marginal electricity production, displaced using batteries, already has a reduced environmental impact. The direct impacts of batteries mainly originate from upstream manufacturing processes, which consume electricity and mining activities related to the extraction of materials such as copper and bauxite.
Knowledge, Practice, and Attitude of Nursing Home Staff Toward the Use of Physical Restraint: Have They Changed Over Time?
Authors: Kor Patrick Pui‐kin , Kwan Rick Yiu Cho , Liu Justina Yat‐Wa , Lai Claudia 
Published: July 2018

AbstractPurposeUse of physical restraints is common in nursing homes, although empirical evidence has shown it to be a hazardous measure. This article aims to understand whether there were any changes in nursing home staff's knowledge, attitudes, and practices of using physical restraints in Hong Kong, after years of deliberation on this topic.MethodsA questionnaire about the knowledge, attitude, and practice of using physical restraint was sent to all 298 staff members in four nursing homes in Hong Kong run by a nongovernmental organization. The results were compared with previous findings from 1999 using the same questionnaire.ResultsOverall, the staff had satisfactory knowledge of the daily application of physical restraints, such as the operational procedure and daily assessment. Concerning the conceptual knowledge of minimizing physical restraint use, their performance in the survey was less satisfactory. For example, only 6.6% of respondents were aware that residents had a right to reject the use of physical restraints, and 70% believed that there were no good alternatives to restraints. They showed appropriate attitudes in their practice of daily use of physical restraints. Compared with the previous study in 1999, a significant improvement was found in the attitudes (p = .0014) and practice (p = .0002) of using restraints, but there was no difference in their knowledge test results (p = .29).ConclusionsThe results of this study show a significant improvement among the nursing home staff in terms of their attitudes and practice of using restraints. In‐service training for nursing staff should focus more on their knowledge of ethical considerations and the hands‐on practice of using alternative devices (e.g., motion detectors or anti‐slip pads).Clinical RelevanceThe findings of this study suggest that in‐service training for nursing staff should focus more on their knowledge of ethical considerations, the principles of using physical restraint, and the alternatives to restraint in order to fill the knowledge gaps of staff and improve the quality of care in nursing homes.
Genomic consequences of a recent three‐way admixture in supplemented wild brown trout populations revealed by local ancestry tracts
Authors: Leitwein Maeva , Gagnaire Pierre‐Alexandre , Desmarais Erick , Berrebi Patrick , Guinand Bruno 
Published: July 2018

AbstractUnderstanding the evolutionary consequences of human‐mediated introductions of domesticated strains into the wild and their subsequent admixture with natural populations is of major concern in conservation biology. However, the genomic impacts of stocking from distinct sources (locally derived vs. divergent) on the genetic integrity of wild populations remain poorly understood. We designed an approach based on estimating local ancestry along individual chromosomes to provide a detailed picture of genomic admixture in supplemented populations. We used this approach to document admixture consequences in the brown trout Salmo trutta, for which decades of stocking practices have profoundly impacted the genetic make‐up of wild populations. In southern France, small local Mediterranean populations have been subject to successive introductions of domestic strains derived from the Atlantic and Mediterranean lineages. To address the impact of stocking, we evaluate the extent of admixture from both domestic strains within populations, using 75,684 mapped SNPs obtained from double‐digested restriction site‐associated DNA sequencing. Then, the chromosomal ancestry profiles of admixed individuals reveal a wider diversity of hybrid and introgressed genotypes than estimated using classical methods for inferring ancestry and hybrid pedigrees. In addition, the length distribution of introgressed tracts retained different timings of introgression between the two domestic strains. We finally reveal opposite consequences of admixture on the level of polymorphism of the recipient populations between domestic strains. Our study illustrates the potential of using the information contained in the genomic mosaic of ancestry tracts in combination with classical methods based on allele frequencies for analysing multiple‐way admixture with population genomic data.
A Method for Automatically Recreating the Horizontal Alignment Geometry of Existing Railways
Authors: Li Wei , Pu Hao , Schonfeld Paul , Song Zhanfeng , Zhang Hong , Wang Lei , Wang Jie , Peng Xianbao , Peng Lihui 
Published: July 2018

AbstractPeriodic recreation of existing railway horizontal alignment geometry is needed for smoothing the deviations arising from train operations. It is important for calibrating track and rebuilding existing railways to ensure safety and comfort. Track calibration repairs the existing distorted track centerline to match the smoothed recreated alignment, which may differ considerably from the originally designed track centerline. Identifying the boundaries of all the geometric elements including tangents, circular curves, and transition curves is the key problem. Existing methods recreate the horizontal alignment semi‐automatically and can only generate a locally optimized solution while considering a few constraints. Based on the principle that the attributions of all the measured points to geometric elements should be consistent with the ranges of recreated geometric elements (i.e., for points‐alignment consistency), a method called swing iterations is proposed to reclassify point placements and identify all the tangents, circular curves, and transition curves simultaneously. In a swing iteration, the boundary of a geometric element segment repeatedly changes from left to right, then from the right to left, and finally stabilizes. Before the swing iterations, preliminary tangents and curves are identified based on the heading gradient (i.e., the rate of change of heading), and are set as initial values for the swing iterations. A genetic algorithm is developed to further refine the entire recreated alignment after the swing iterations. In the above processes, multiple constraints are handled. Applications demonstrate that this method can identify all horizontal geometric elements automatically and generate an optimized recreated alignment geometry for an existing railway while satisfying all the applicable constraints.
Leukotriene A4 hydrolase: an emerging target of natural products for cancer chemoprevention and chemotherapy
Authors: Vo Tam Thuy Lu , Jang Won‐Jun , Jeong Chul‐Ho 
Published: July 2018

AbstractCancer is the second leading cause of death worldwide and has become a global burden. It has long been known that inflammation is related to cancer, as inflammatory components have been identified in the tumor microenvironment and support tumor progression. Among the key inflammatory mediators, leukotrienes were found to be involved in cancer development. In particular, leukotriene B4, which is converted from leukotriene A4 by leukotriene A4 hydrolase (LTA4H), has been implicated in several types of cancer. In addition, LTA4H has attracted attention because of purported roles in inflammation and cancer development. Herein, we review the history of LTA4H, its emerging roles in cancer development, and the development of LTA4H inhibitors in cancer prevention and therapy.
Influence of omega‐3 incorporation in sperm preservation medium on physical and kinematic properties of chilled and cryopreserved ram spermatozoa
Authors: Rateb Sherif A. 
Published: July 2018

ContentsTwo experiments were carried out to investigate the efficiency of supplementing sperm preservation medium with omega‐3 polyunsaturated fatty acids on improving liquid‐chilled storage and cryopreservation capacity of ram spermatozoa. Ejaculates (n = 100) were collected from five adult rams, Ovis aries, by an artificial vagina twice weekly throughout the period February–April, 2017. After initial evaluation, ejaculates of each collection session from the same males were pooled, diluted (1:10) with Tris‐citric acid egg yolk extender, and were further split into five aliquots using a split‐sample technique. The first aliquot served as control (omega‐free), whereas the other four portions were supplemented with 0.1, 0.2, 0.3 or 0.4 mM omega‐3, respectively (T0). Thereafter, the diluted specimens were stored at 4°C for 48 hr, during which sperm physical and morphometric properties were evaluated along with oxidative stress indices (T24, T48). Omega‐3 levels that efficiently mitigated the detrimental effects of chilled preservation, and maintained preservation aptitude of spermatozoa were further investigated for sperm cryosurvival against control (untreated). Post‐thaw physical and kinematic properties of spermatozoa, in all groups, were objectively evaluated by a computer‐assisted sperm analysis (CASA) system. The results showed that, at 48 hr of chilled storage, supplementing preservation medium with 0.4 mM omega‐3 was positively correlated (p < 0.01) with each of progressive motility, live sperm, intact acrosome and intact cell membrane (r = 0.83, 0.85, 0.85, 0.89, respectively). Furthermore, a positive correlation (p < 0.01) was observed between inclusion of omega‐3 in cryopreservation medium and each of post‐thaw total sperm motility, progressive motility, live sperm, normal sperm, intact acrosome, intact cell membrane, VCL, VSL, VAP, ALH and STR (r = 0.76, 0.84, 0.79, 0.90, 0.89, 0.91, 0.61, 0.73, 0.65, 0.78 and 0.60, respectively). These results accentuate efficiency of supplementing the diluent with omega‐3 fatty acids on improving chilled and cryopreservation aptitude of ram spermatozoa.
Association between the expression of miR‐26 and goat milk fatty acids
Authors: Wang Hui , Zhu Jiangjiang , He Qiuya , Loor Juan J. , Luo Jun 
Published: July 2018

ContentsmicroRNA (miRNA) are small noncoding RNA that regulate protein abundance and are involved in diverse aspects of cellular function including aspects of lipid metabolism in mammary gland of ruminants. Although our previous studies showed that the miR‐26 family and its host genes control components of the cellular fatty acid metabolic machinery in goat mammary epithelial cells, a direct relationship between the miR‐26 family and milk fatty acids remains unknown. Bioinformatics analysis in this study indicated that the miR‐26 family targets belong to the PI3K‐Akt signalling pathway, MAPK signalling pathway, and fatty acid biosynthesis pathway. Studies on the relationship of miR‐26 family and their host genes with milk composition during mid‐lactation revealed that the expression of the miR‐26 family and their host genes were associated with total fat yield and short‐chain, medium‐chain and long‐chain fatty acid content, but not lactose or milk protein content. In addition, a significant positive correlation was detected for the expression of the miR‐26 family with C16:1 and C18:3 in milk fat. Taken together, our findings demonstrate that the expression of miR‐26 is directly related to milk fatty acid composition and underscores the significance of miRNAs in milk fat synthesis regulation.
Expression of the Toarcian Oceanic Anoxic Event: New insights from a Swiss transect
Authors: Fantasia Alicia , Föllmi Karl B. , Adatte Thierry , Spangenberg Jorge E. , Mattioli Emanuela 
Published: July 2018

AbstractA sedimentological, biostratigraphical and geochemical (stable isotopes and Rock‐Eval parameters) analysis was performed on four Swiss successions, in order to examine the expression of the Toarcian Oceanic Anoxic Event along a north–south transect, from the Jura through the Alpine Tethys (Sub‐Briançonnais and Lombardian basins). The locations were selected to represent a range of palaeoceanographic positions from an epicontinental sea to a more open marine setting. The Toarcian Oceanic Anoxic Event was recognized by the presence of the characteristic negative carbon‐isotope excursion in carbonate (ca 2 to 4‰) and organic matter (ca 4 to 5‰) at the base of the falciferum ammonite Zone (NJT6 nannofossil Zone). The sedimentary expression of the Toarcian Oceanic Anoxic Event varies along the transect from laminated mudstone rich in total organic carbon (≤11 wt.%) in the Jura, to thin‐bedded marl (≤5 wt.% total organic carbon) in the Sub‐Briançonnais Basin and to hemipelagic reddish marly limestone (total organic carbon <0·05 wt.%) in equivalent levels from the Lombardian Basin. The carbon‐isotope excursion is thus independent of facies and palaeoceanographic position. The low nannofossil abundance and the peak in Calyculaceae in the Jura and the Sub‐Briançonnais Basin indicate low salinity surface waters and stratified water masses in general. Sedimentological observations (for example, obliquely‐bedded laminae and homogeneous mud layers containing rip‐up clasts) indicate the presence of dynamic conditions, suggesting that water mass stratification was episodically disrupted during the Toarcian Oceanic Anoxic Event. The proposed correlation highlights a stratigraphic gap and/or condensed interval between the Pliensbachian–Toarcian boundary and the Toarcian Oceanic Anoxic Event interval (most of the tenuicostatum ammonite Zone is missing), which is also observed in coeval European sections and points to the influence of sea‐level change and current dynamics. This transect shows that the sedimentary expression of the Toarcian Oceanic Anoxic Event is not uniform across the Alpine Tethys, supporting the importance of local conditions in determining how this event is recorded across different palaeoceanographic settings.
A molecular phylogeny of the tribe Ochthebiini (Coleoptera, Hydraenidae, Ochthebiinae)
Authors: Villastrigo Adrián , Jäch Manfred A. , Cardoso Anabela , Valladares Luis F. , Ribera Ignacio 
Published: July 2018

AbstractOchthebiinae, with c. 650 species distributed worldwide, are the second most speciose subfamily of the aquatic beetle family Hydraenidae. They are ecologically the most diverse hydraenid subfamily, with terrestrial species as well as species in almost all types of aquatic habitats, including hypersaline waters. Ochthebiinae include the tribes Ochtheosini (four species in three genera) and Ochthebiini. We provide here the first comprehensive phylogeny of the tribe Ochthebiini, based on 186 species and four subspecies from most genera, subgenera and species groups. We obtained sequence data for a combination of mitochondrial and nuclear gene fragments including the 5′ and 3′ ends of the cytochrome c oxidase subunit 1, the 5′ end of 16S RNA plus the leucine tRNA transfer plus 5′ end of NADH dehydrogenase subunit I, and internal fragments of the large and small ribosomal units. The analyses with maximum likelihood (ML) and Bayesian probabilities consistently recovered a generally well supported phylogeny, with most currently accepted taxa and species groups as monophyletic. We provide a new classification of the tribe based on our phylogenetic results, with six genera: Meropathus Enderlein, Ochthebius Leach, Protochthebius Perkins, Prototympanogaster Perkins, Tympallopatrum Perkins and Tympanogaster Janssens. The genus Ochthebius is here divided into nine subgenera in addition to Ochthebius s.s.: (1) O. (Angiochthebius) Jäch & Ribera; (2) O. (Asiobates) Thomson; (3) O. (Aulacochthebius) Kuwert; (4) O. (Cobalius) Rey; (5) O. (Enicocerus) Stephens; (6) O. (Gymnanthelius) Perkins comb.n.; (7) O. (Gymnochthebius) Orchymont; (8) O. (Hughleechia) Perkins comb.n.; and (9) O. (Micragasma) Sahlberg. Within Ochthebius s.s., 17 species groups are proposed, five of them newly established (3, 9, 11, 13 and 16): (1) andraei; (2) atriceps; (3) corrugatus; (4) foveolatus; (5) kosiensis; (6) lobicollis; (7) marinus; (8) metallescens; (9) nitidipennis; (10) notabilis; (11) peisonis; (12) punctatus; (13) quadricollis; (14) rivalis; (15) strigosus; (16) sumatrensis; and (17) vandykei. We elevated to species rank two subspecies of Ochthebius: O. fallaciosus Ganglbauer stat.n. (former subspecies of O. viridis Peyron) and O. deletus Rey stat.rest. (former subspecies of O. subpictus Wollaston).