bluuebunny/crossref_metadata_2025_split huggingface dataset:
Goal-Directed and Habitual Control in Smokers
Authors: Luijten Maartje , Gillan Claire M , de Wit Sanne , Franken Ingmar H A , Robbins Trevor W , Ersche Karen D 
Published: January 2019

Abstract

Introduction
Harmful behavior such as smoking may reflect a disturbance in the balance of goal-directed and habitual control. Animal models suggest that habitual control develops after prolonged substance use. In this study, we investigated whether smokers (N = 49) differ from controls (N = 46) in the regulation of goal-directed and habitual behavior. It was also investigated whether individual differences in nicotine dependence levels were associated with habitual responding.

Methods
We used two different multistage instrumental learning tasks that consist of an instrumental learning phase, subsequent outcome devaluation, and a testing phase to measure the balance between goal-directed and habitual responding. The testing phases of these tasks occurred after either appetitive versus avoidance instrumental learning. The appetitive versus aversive instrumental learning stages in the two different tasks modeled positive versus negative reinforcement, respectively.

Results
Smokers and nonsmoking controls did not differ on habitual versus goal-directed control in either task. Individual differences in nicotine dependence within the group of smokers, however, were positively associated with habitual responding after appetitive instrumental learning. This effect seems to be due to impaired stimulus-outcome learning, thereby hampering goal-directed task performance and tipping the balance to habitual responding.

Conclusions
The current finding highlights the importance of individual differences within smokers. For future research, neuroimaging studies are suggested to further unravel the nature of the imbalance between goal-directed versus habitual control in severely dependent smokers by directly measuring activity in the corresponding brain systems.

Implications
Goal-directed versus habitual behavior in substance use and addiction is highly debated. This study investigated goal-directed versus habitual control in smokers. The findings suggest that smokers do not differ from controls in goal-directed versus habitual control. Individual differences in nicotine dependence within smokers, however, were positively associated with habitual responding after appetitive instrumental learning. This effect seems to be due to impaired stimulus-outcome learning, thereby hampering goal-directed task performance and tipping the balance to habitual responding. These findings add to the ongoing debate on habitual versus goal-directed control in addiction and emphasize the importance of individual differences within smokers.
Frameworks and guidance to support ethical public health practice
Authors: Viens A M , Vass Caroline 
Published: January 2019

Abstract
This article reports and reflects on an element of a recent survey of UK public health professionals, specifically in relation to the Public Health Knowledge and Skills Framework (PHSKF) and the ethical requirements that underpin public health practice. Only 38.4% of respondents reported accessing the PHKSF and a mere 13.7% reported accessing the accompanying background paper on ethical public health practice. Given that ethical practice underpins the PHSKF, it is concerning that so few respondents are familiar with the PHSKF and one of the source documents. While issuing frameworks and guidance is one way to support public health practice, there is a further need for greater integration of skills and knowledge around ethical public health practice within education and training initiatives.
Heritability of Cerebral Blood Flow and the Correlation to Schizophrenia Spectrum Disorders: A Pseudo-continuous Arterial Spin Labeling Twin Study
Authors: Legind Christian S , Broberg Brian V , Brouwer Rachel , Mandl René C W , Ebdrup Bjørn H , Anhøj Simon J , Jensen Maria H , Hilker Rikke , Fagerlund Birgitte , Hulshoff Pol Hilleke E , Glenthøj Birte Y , Rostrup Egill 
Published: January 2019

AbstractWhether aberrant cerebral blood flow (CBF) in schizophrenia is affected by genetic influences, and consequently a potential marker for genetic susceptibility, is unknown. Our aims were to determine the heritability of CBF in thalamic, frontal, and striatal areas, and to ascertain if associations with disease were under genetic influence. Monozygotic (MZ) twin pairs concordant (n = 2) or discordant (n = 20) for schizophrenia spectrum disorders (ICD-10 F2x.x), matched on sex and age with dizygotic (DZ; n = 20) and healthy control pairs (MZ: n = 27; DZ: n = 18; total: n = 181 individuals), were recruited via the National Danish Twin Register. CBF in thalamus, frontal lobes, and putamen was measured with pseudo-continuous arterial spin labeling on a 3 T magnetic resonance scanner. Twin statistics were performed with structural equation modeling. CBF in the frontal lobes was heritable (h2 = 0.44, 95% CI [0.22–0.60]) but not correlated to disease. CBF correlated to schizophrenia spectrum disorders in the left thalamus (r = 0.17, [0.03–0.31]; P = 0.02), as well as in the left putamen (r = 0.19, [0.05–0.32]; P = 0.007) and the right putamen (r = 0.18, [0.03–0.32]; P = 0.02). When restricting the sample to schizophrenia (F20.x) only, shared genetic influences between CBF in the left putamen and schizophrenia liability (phenotypic correlation = 0.44, [0.28–0.58], P < 0.001) were found. Our results provide heritability estimates of CBF in the frontal lobes, and we find CBF in thalamus and putamen to be altered in schizophrenia spectrum disorders. Furthermore, shared genetic factors influence schizophrenia liability and striatal perfusion. Specifically, higher perfusion in the left putamen may constitute a marker of genetic susceptibility for schizophrenia.
An educational module to explore CRISPR technologies with a cell-free transcription-translation system
Authors: Collias Daphne , Marshall Ryan , Collins Scott P , Beisel Chase L , Noireaux Vincent 
Published: January 2019

Abstract
Within the last 6 years, CRISPR-Cas systems have transitioned from adaptive defense systems in bacteria and archaea to revolutionary genome-editing tools. The resulting CRISPR technologies have driven innovations for treating genetic diseases and eradicating human pests while raising societal questions about gene editing in human germline cells as well as crop plants. Bringing CRISPR into the classroom therefore offers a means to expose students to cutting edge technologies and to promote discussions about ethical questions at the intersection of science and society. However, working with these technologies in a classroom setting has been difficult because typical experiments rely on cellular systems such as bacteria or mammalian cells. We recently reported the use of an E. coli cell-free transcription-translation (TXTL) system that simplifies the demonstration and testing of CRISPR technologies with shorter experiments and limited equipment. Here, we describe three educational modules intended to expose undergraduate students to CRISPR technologies using TXTL. The three sequential modules comprise (i) designing the RNAs that guide DNA targeting, (ii) measuring DNA cleavage activity in TXTL and (iii) testing how mutations to the targeting sequence or RNA backbone impact DNA binding and cleavage. The modules include detailed protocols, questions for group discussions or individual evaluation, and lecture slides to introduce CRISPR and TXTL. We expect these modules to allow students to experience the power and promise of CRISPR technologies in the classroom and to engage with their instructor and peers about the opportunities and potential risks for society.
Salivary IgG levels in neonatal calves and its association to serum IgG: an observational pilot study
Authors: Johnsen Julie Føske , Chincarini Matteo , Sogstad Åse Margrethe , Sølverød Liv , Vatne Marie , Mejdell Cecilie Marie , Hänninen Laura 
Published: January 2019

AbstractThe diagnosis of inadequate transfer of colostrum immunoglobulin G (IgG) to calf serum, often known as failure of passive transfer (&lt;10 g/L IgG1 at 24 to 48 h), necessitates blood sampling from the calf and in some instances the presence of a veterinarian. Sampling saliva is both less invasive and easy for the producer. Previous research has shown that quantification of saliva IgG is possible in juvenile and adult cattle. The objectives of this observational pilot study were to investigate whether IgG can be quantified in neonatal calf saliva, if it is correlated to serum IgG concentrations, and if the indirect quantification of saliva IgG is achievable by use of a digital refractometer. Paired blood and saliva samples were collected from 20 healthy dairy calves aged 1 to 3 d. In these samples, IgG was quantified directly with single radial immunodiffusion and indirectly by use of a digital refractometer indicating Brix % (a subsample of n = 12 saliva samples). A strong positive correlation (r = 0.7, P &lt; 0.001) between saliva IgG (mean ± SD; 0.2 ± 0.11 g/L) and serum IgG (32.1 ± 11.94 g/L) was found. Saliva IgG ranged from the lowest detectable value, 0.1 g/L (n = 6 samples) to 0.6 g/L. Saliva Brix (1.2 ± 0.69%) was not significantly correlated to serum IgG (n = 12, r = 0.43, P = 0.155); however, it was significantly correlated to saliva IgG (n = 12, r = 0.7, P = 0.018) and Brix in serum (n = 12, r = 0.7, P = 0.013). We conclude that IgG was quantifiable in most of the saliva samples. For saliva IgG to be of any value with regards to detecting failure of passive transfer, future studies should investigate methods that can detect IgG &lt;0.1 g/L. The results indicate that saliva IgG can be used to predict serum IgG at levels above 10 g/L, which may warrant further exploration of the use of saliva in the surveillance of failure of passive transfer. The results of the current pilot study did not support the potential usage of a Brix % refractometer to quantify saliva IgG.
Unexpected diversity of Hyboserica chafers in South African forest remnants: cladistic analysis, new species and the new genus Leoserica (Coleoptera: Scarabaeidae: Melolonthinae)
Authors: Fabrizi Silvia , Eberle Jonas , Ahrens Dirk 
Published: January 2019

AbstractA taxonomic revision of Hyboserica uncovers an unexpected diversity of species from Southern African forest remnants. The work results in the description of a new genus, Leoserica gen. nov., a new combination, a new synonymy and 32 new species are described. The lectotypes of Serica capensis and Triodonta caffra are designated. The habitus and male genitalia of all revised species are illustrated. A species distribution map and an identification key to the species are provided. Phylogenetic analysis reveals a deep split into one very diverse eastern clade that is distributed north to Zimbabwe, and one western clade that is restricted to the fynbos of the Cape Region.
Outcomes Analysis of Gynecologic Oncologic Reconstruction
Authors: Block Lisa M. , Hartmann Emily C. , King Jason , Chakmakchy Saygin , King Timothy , Bentz Michael L. 
Published: January 2019

Background:
Defects resulting from gynecologic oncology resections can range from small external defects to total exenterations, requiring complex pelvic reconstruction. We aim to investigate the patient and surgical factors that influence complication rates, reoperation rates, and length of stay. We hypothesize that this patient cohort will have high complication and reoperation rates that are likely most affected by their medical and extirpative surgery factors, with less direct impact from their reconstructive surgery procedures.

Methods:
All cases of reconstruction following resection of a gynecological oncology tumor at the University of Wisconsin Hospital over the last 14 years were reviewed. Forty-three patients were identified who required 66 flaps for reconstruction.

Results:
Mean follow-up period was 19 months. Overall complication rate was 65% and reoperation rate was 33%. Plastic surgery flap-specific complication and reoperation rates were 47% and 19%, respectively, and were not significantly associated with any patient risk factors. Flap reconstruction subtype was not associated with time to complete healing, complication rate, or reoperation. Prior chemotherapy was significantly correlated with increased rate of overall complication (P = 0.0253) and reoperation (P = 0.0448), but prior radiation was not. Mean hospital stay was 11 days (SD ± 9 d). Factors found to be significantly associated with an increase in hospitalization length were increasing number of comorbidities (P = 0.021), exenteration defects (P = 0.0122), myocutaneous flap reconstruction (P = 0.0003), radiation (P = 0.0004), and chemotherapy P = 0.0035).

Conclusion:
This patient cohort has an overall high complication and reoperation rate; however, increasingly complex reconstruction is not associated with significant differences in complication rates or reoperation.
The Effect of Marked Nasal Septal Deviation on Left Ventricular Function and Blood Markers
Authors: Karataş Mehmet , Taşolar Hakan 
Published: January 2019

Objectives:This study aimed to assess the effect of nasal septoplasty in patients with marked nasal septal deviation (MNSD) on subjective perception of nasal patency, echocardiography, and hematologic parameters.Methods:Seventy-nine consecutive patients (mean age, 29.9 ± 9.7 years; 57 men and 22 women) at least 18 years old were diagnosed as MNSD consistent with presenting symptom of chronic nasal obstruction lasting at least 3 months. Complete blood count measurement to assess mean platelet volume (MPV) and neutrophil to lymphocyte ratio (NLR), the Nasal Obstruction Symptom Evaluation (NOSE) questionnaire, and echocardiography were performed for all patients just before and at postoperative third month of septoplasty.Results:The MPV (0.021), NLR (<0.001), and NOSE (<0.001) were all significantly decreased, and global longitudinal strain (GLS) value (<0.001) were also significantly increased at postoperative third month. Correlation analysis also indicated that ΔNLRwas positively correlated with ΔGLS(r = 0.385,P < 0.001), and ΔNOSE(r = 0.436,P < 0.001), and correlated negatively with the ΔMPV(r = –0.302,P = 0.024). ΔGLSwas also positively correlated with the ΔNOSE(r = 0.769,P < 0.001).Conclusion:Septoplasty for MNSD could provide not only alleviation of otorhinolaryngologic symptoms but also improvement in subclinical left ventricular systolic dysfunction and decline in MPV and NLR levels.
Seizures Following Cranioplasty: Risk Factors and Prevention Exploration
Authors: Zhang Jian , Liu Xianghui , Zhou Jun , Zhang Zhiying , Fu Meizhi , Guo Yunlin , Li Gang 
Published: January 2019

Background:
The aim of this study was to identify risk factors and explore the possible prevention measures for seizures following cranioplasty.

Methods:
The authors performed a retrospective review of 142 consecutive patients who underwent cranioplasty following craniectomy for trauma or cerebral hemorrhage in Dezhou People's Hospital between January 2010 and January 2017. Patients who were diagnosed with aneurysms, arteriovenous malformations, cerebral infarction, and tumors (14), had seizures prior to cranioplasty (9) and those lost to follow-up (7) were excluded. Patients did not use antiepilepsy drugs from treatment of postcranioplasty seizures. The median follow-up time was 51.11 ± 31.59 months (range: 17–98 months). Analyses were performed on a database tracking age, sex, reason for craniectomy, operative time, time between operations, presence of dural substitute, diabetic status, hypertensive status, tobacco use, alcohol use, location of cranioplasty, classification, time and times of seizures following cranioplasty development.

Results:
One hundred twelve patients met study criteria. The overall rate of seizures following cranioplasty was 35.7% (40 out of 112 patients). There were no statistically significant associations between postcranioplasty seizures and sex, age, location of cranioplasty, cranioplasty materials, or dural substitute used in craniectomy. Postcranioplasty seizure frequency differed significantly according to reasons for depressive craniectomy. The incidence of postcranioplasty seizures was significantly higher in the first year than in later years. Incidence decreased progressively in subsequent years.

Conclusion:
Incidence of seizures following cranioplasty was associated with the reason for depressive craniectomy.
Proof simplification and automated theorem proving
Authors: Kinyon Michael 
Published: January 2019

The proofs first generated by automated theorem provers are far from optimal by any measure of simplicity. In this paper, I describe a technique for simplifying automated proofs. Hopefully, this discussion will stimulate interest in the larger, still open, question of what reasonable measures of proof simplicity might be.
This article is part of the theme issue ‘The notion of ‘simple proof’ - Hilbert's 24th problem’.
Prolegomena to any theory of proof simplicity
Authors: Pambuccian Victor 
Published: January 2019

By looking at concrete examples from elementary geometry, we analyse the manner in which the simplicity of proofs could be defined. We first find that, when presented with two proofs coming from mutually incompatible sets of assumptions, the decision regarding which one is simplest can be made, if at all, only on the basis of reasoning outside of the formal aspects of the axiom systems involved. We then show that, if the axiom system is fixed, a measure of proof simplicity can be defined based on the number of uses of axioms deemed to be deep or valuable, and prove a number of new results regarding the need to use at least three times some axioms in the proof of others. One such major example is Pappus implies Desargues, which is shown to require three uses of Pappus. A similar situation is encountered with Veblen's proof that the outer form of the Pasch axiom implies the inner form thereof. The outer form needs to be used at least three times in any such proof. We also mention the likely conflicting requirements of directness of a proof and the length of a proof.
This article is part of the theme issue ‘The notion of ‘simple proof’ - Hilbert's 24th problem’.
scSLAM-seq reveals core features of transcription dynamics in single cells
Authors: Erhard Florian , Baptista Marisa A.P. , Krammer Tobias , Hennig Thomas , Lange Marius , Arampatzi Panagiota , Jürges Christopher , Theis Fabian J. , Saliba Antoine-Emmanuel , Dölken Lars 
Published: January 2019

AbstractCurrent single-cell RNA sequencing approaches gives a snapshot of a cellular phenotype but convey no information on the temporal dynamics of transcription. Moreover, the stochastic nature of transcription at molecular level is not recovered. Here, we present single-cell SLAM-seq (scSLAM-seq), which integrates metabolic RNA labeling, biochemical nucleoside conversion and single-cell RNA-seq to directly measure total transcript levels and transcriptional activity by differentiating newly synthesized from pre-existing RNA for thousands of genes per single cell. scSLAM-seq recovers the earliest virus-induced changes in cytomegalovirus infection and reveals a so far hidden phase of viral gene expression comprising promiscuous transcription of all kinetic classes. It depicts the stochastic nature of transcription and demonstrates extensive gene-specific differences. These range from stable transcription rates to on-off dynamics which coincide with gene-/promoter-intrinsic features (Tbp-TATA-box interactions and DNA methylation). Gene but not cell-specific features thus explain the heterogeneity in transcriptomes between individual cells and the transcriptional response to perturbations.
Genomewide Analyses of Psychological Resilience in US Army Soldiers
Authors: Stein Murray B. , Choi Karmel W. , Jain Sonia , Campbell-Sills Laura , Chen Chia-Yen , Gelernter Joel , He Feng , Heeringa Steven G. , Maihofer Adam X. , Nievergelt Caroline M. , Nock Matthew K. , Ripke Stephan , Sun Xiaoying , Kessler Ronald C. , Smoller Jordan W. , Ursano Robert J. 
Published: January 2019

ABSTRACTThough a growing body of preclinical and translational research is illuminating a biological basis for resilience to stress, little is known about the genetic basis of psychological resilience in humans. We conducted genomewide association studies (GWAS) of self-assessed (by questionnaire) and outcome-based (incident mental disorders from pre- to post-deployment) resilience among European (EUR) ancestry soldiers in the Army Study To Assess Risk and Resilience in Servicemembers (STARRS). Self-assessed resilience (N=11,492) was found to have significant common-variant heritability (h2=0.162, se=0.050, p=5.37e-4), and to be significantly negatively genetically correlated with neuroticism (rg= −0.388, p=0.0092). GWAS results from the EUR soldiers revealed a genomewide significant locus (4 SNPs in LD; top SNP: rs4260523, p=5.654e-09) on an intergenic region on Chr 4 upstream from DCLK2 (Doublecortin-Like Kinase 2), a member of the doublecortin (DCX) family of kinases that promote survival and regeneration of injured neurons. A second gene, KLHL36 (Kelch Like Family Member 36) was detected at gene-wise genomewide significance (p=1.89e-06). A polygenic risk score derived from the self-assessed resilience GWAS was not significantly associated with outcome-based resilience. In very preliminary results, genomewide significant association with outcome-based resilience was found for one locus (top SNP: rs12580015) on Chr 12 downstream from SLC15A5 (solute carrier family 15 member 5) in the small group (N=581) of subjects exposed to the highest level of deployment stress. The further study of genetic determinants of resilience has the potential to illuminate the molecular bases of stress-related psychopathology and potentially point to new avenues for therapeutic intervention.