bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adaptations in hepatic glucose metabolism after chronic social defeat stress in mice
Authors: Meijboom Fabiënne S. , Hasch Annika , Ruiz de Azua Inigo , Cologna Camila Takeno , Loopmans Shauni , Lutz Beat , Müller Marianne B. , Ghesquière Bart , van der Kooij Michael A. 
Published: October 2024

AbstractChronic stress has been shown to induce hyperglycemia in both peripheral blood and the brain, yet the detailed mechanisms of glucose metabolism under stress remain unclear. Utilizing 13C6-labeled glucose to trace metabolic pathways, our study investigated the impact of stress by chronic social defeat (CSD) on glucose metabolites in the liver and brain one week post-stress. We observed a reduction in 13C6-enrichment of glucose metabolites in the liver, contrasting with unchanged levels in the brain. Notably, hepatic glycogen levels were reduced while lactate concentrations were elevated, suggesting lactate as an alternative energy source during stress. Long-term effects were also examined, revealing normalized blood glucose levels and restored glycogen stores in the liver three weeks post-CSD, despite sustained increases in food intake. This normalization is hypothesized to result from diminished glucagon levels leading to reduced glycogen phosphorylase activity. Our findings highlight a temporal shift in glucose metabolism, with hyperglycemia and glycogen depletion in the liver early after CSD, followed by a later phase of metabolic stabilization. These results underscore the liver’s critical role in adapting to CSD and provide insights into the metabolic adjustments that maintain glucose homeostasis under prolonged stress conditions.
Aboriginal Australian weapons and human efficiency
Authors: Diamond Laura E. , Langley Michelle C. , Cornish Bradley , Pizzolato Claudio , Saxby David J. 
Published: October 2024

AbstractAggression—and its role in human societal development—continues to be hotly debated within both the sciences and the humanities. Whatever the evolutionary origins and repercussions of interpersonal and intergroup conflict for the human story, cultures around the globe have invested significant time and effort into designing deadly hand-held weaponry. Here, we describe for the first time, how humans deliver a deadly strike using two iconic and widespread Aboriginal Australian weapons: the kodj and the leangle with parrying shield. We present the world’s first evaluation of striking biomechanics and human and weapon efficiency regarding this class of implement. Results demonstrate the leangle is far more effective at delivering devastating blows to the human body, while the kodj—a multi-functional tool—is more efficient for a human to manoeuvre and still capable of delivering severe blows that can cause death. Together, these data provide the beginnings of an in-depth understanding of how hand-held weaponry has impacted the human body throughout the deep past.
Transpulmonary chemoembolization and microwave ablation for recurrent or advanced non-small cell Lung Cancer
Authors: Vogl Thomas J. , Chen Kuei-An , Li Hao , Fouad Mohamed E.M. , Afraz Zahra , Adwan Hamzah 
Published: October 2024

AbstractTo verify the treatment effect of the combination of transpulmonary chemoembolization (TPCE) and microwave ablation (MWA), targeting the treatment of recurrent or advanced non-small cell lung cancer (NSCLC). A total of 53 patients were studied and grouped according to the diameter of the largest pulmonary nodule, defined as index tumor size (ITS). Patients with an ITS > 3 cm (n = 20) were treated with TPCE and MWA. Patients with an ITS ≤ 3 cm were treated either with a combination therapy (n = 24) or MWA alone (n = 9). The treatment response, including complications and survival outcome, was then analyzed. After TPCE, there was an average ITS reduction of 0.91 cm, and 25% of patients in ITS > 3 cm were downgraded to ITS ≤ 3 cm. After TPCE, there were 12 patients (27%) with PR status and 32 (73%) with SD status. No PD patient in our case series was noted before MWA.The complication rate of MWA was significantly higher in ITS ≤ 3 cm than in ITS > 3 cm (p = 0.013). The median survival time (MST) was 26.7 months, and the time to progression was 13.2 months. The patients in the ITS ≤ 3 cm had longer MST than the others (31.6 vs. 15.8 months, p = 0.003). The significant prognostic factor was ITS > 3 cm (HR: 1.18, p = 0.02). A combination of TPCE and MWA might be feasible to control non-operable, recurrent, or advanced NSCLC.
Perceptual discrimination of action formidableness and friendliness and the impact of autistic traits
Authors: Vlasceanu Alessia M. , de la Rosa Stephan , Barraclough Nick E. 
Published: October 2024

AbstractThe ability to determine whether the actions of other individuals are friendly or formidable are key decisions we need to make to successfully navigate our complex social environment. In this study we measured perceptual performance when discriminating actions that vary in their friendliness or formidableness, and whether performance was related to the autistic traits of individuals. To do this, we developed an action morphing method to generate novel actions that lied along the action quality dimensions of formidableness and friendliness. In Experiment 1 we show that actions that vary along the formidableness or friendliness continua were rated as varying monotonically along the respective quality. In Experiment 2 we measured the ability of individuals with different levels of autistic traits to discriminate action formidableness and friendliness using adaptive 2-AFC procedures. We found considerable variation in perceptual thresholds when discriminating action formidableness (~ 540% interindividual variation) or friendliness (~ 1100% interindividual variation). Importantly, we found no evidence that autistic traits influenced perceptual discrimination of these action qualities. These results confirm that sensory enhancements with autistic traits are limited to lower level stimuli, and suggest that the perceptual processing of these complex social signals are not affected by autistic traits.
One-year outcomes of a digital twin intervention for type 2 diabetes: a retrospective real-world study
Authors: Shamanna Paramesh , Erukulapati Ravi Sankar , Shukla Ashutosh , Shah Lisa , Willis Bree , Thajudeen Mohamed , Kovil Rajiv , Baxi Rahul , Wali Mohsin , Damodharan Suresh , Joshi Shashank 
Published: October 2024

AbstractThis retrospective observational study, building on prior research that demonstrated the efficacy of the Digital Twin (DT) Precision Treatment Program over shorter follow-up periods​​, aimed to examine glycemic control and reduced anti-diabetic medication use after one-year in a DT commercial program. T2D patients enrolled had adequate hepatic and renal function and no recent cardiovascular events. DT intervention powered by artificial intelligence utilizes precision nutrition, activity, sleep, and deep breathing exercises. Outcome measures included HbA1c change, medication reduction, anthropometrics, insulin markers, and continuous glucose monitoring (CGM) metrics. Of 1985 enrollees, 132 (6.6%) were lost to follow-up, leaving 1853 participants who completed one-year. At one-year, participants exhibited significant reductions in HbA1c [mean change: -1.8% (SD 1.7%), p < 0.001], with 1650 (89.0%) achieving HbA1c below 7%. At baseline, participants were on mean 1.9 (SD 1.4) anti-diabetic medications, which decreased to 0.5 (SD 0.7) at one-year [change: -1.5 (SD 1.3), p < 0.001]. Significant reductions in weight [mean change: -4.8 kg (SD 6.0 kg), p < 0.001], insulin resistance [HOMA2-IR: -0.1 (SD 1.2), p < 0.001], and improvements in β-cell function [HOMA2-B: +21.6 (SD 47.7), p < 0.001] were observed, along with better CGM metrics. These findings suggest that DT intervention could play a vital role in the future of T2D care.
The prominent pervasive oncogenic role and tissue specific permissiveness of RAS gene mutations
Authors: Yi Ming , Soppet Daniel , McCormick Frank , Nissley Dwight V. 
Published: October 2024

AbstractIn cancer research, RAS biology has been focused on only a handful of tumor types. While RAS genes have long been suspected as common contributors to a wide spectrum of cancer types, robust evidence is required to firmly establish their critical oncogenic significance. We present a data mining study using DepMap genome-wide CRISPR screening data, which provide substantial evidence to support the prominent pervasive oncogenic role and tissue-specific permissiveness of RAS gene mutations. Differential analysis of CRISPR effect scores identifies K- or N-RAS genes as the most differential gene in contrasts of (K-, N-, combined) RAS mutant versus wild-type cell lines across multiple tissue types. The distinguished tissue-specific pattern of KRAS vs. NRAS as top differential genes in subsets of tissue types and evidence from genome data supported the idea of KRAS- and NRAS-engaged tissue types. To our knowledge, this is the first report of prominent pervasive oncogenic role of RAS mutations revealed by gene dependency data that is beyond the current understanding of the oncogenic role of RAS genes and their well-known involved tissue types. Our findings strongly support RAS mutations as primary oncogenic drivers beyond traditionally recognized cancer types and offer insights into their tissue-specific permissiveness.
Brazilian kefir fraction mitigates the Alzheimer-like phenotype in Drosophila melanogaster with β-amyloid overexpression model
Authors: Malta Serena Mares , Rodrigues Tamiris Sabrina , Silva Matheus Henrique , Marquez Alexandre Souza , Ferreira Rafael Bernardes , do Prado Mascarenhas Fernanda Naves Araújo , Zanon Renata Graciele , Bernardes Lucas Matos Martins , Batista Letícia Leandro , da Silva Murillo Néia Thomaz , de Oliveira Santos Débora , Santos Ana Carolina Costa , Mendes-Silva Ana Paula , Spindola Foued Salmen , Ueira-Vieira Carlos 
Published: October 2024

AbstractAlzheimer’s disease (AD) is a progressive neurodegenerative condition and the primary form of dementia among elderly people. The amyloidogenic hypothesis is the main theory that explains this phenomenon and describes the extracellular accumulation of amyloid beta (Aβ) peptides. Model organisms such as Drosophila melanogaster have been utilized to improve the understanding of this disease and its treatment. This study evaluated the effects of peptide and metabolic fractions of Brazilian kefir on a strain of D. melanogaster that expresses human Aβ peptide 1–42 in the eye. The parameters assessed included ommatidial organization, vacuole area, retinal thickness, and Aβ peptide quantification. The present study revealed that the fractions, particularly the peptidic fraction, significantly reduced the vacuole area and increased the retina thickness in treated flies, indicating an improvement in neurodegeneration phenotype. The peptidic fraction was also found to alter Aβ aggregation dynamics, inhibiting Aβ fibril formation, as revealed by dynamic light scattering. This study demonstrated that kefir fractions, particularly the peptidic fraction < 10 kDa, have the potential to regulate Aβ aggregation and alleviate neurodegeneration in a Drosophila melanogaster AD-like model. These findings suggest that kefir fractions could be viable for the bioprospection of novel drug prototypes for AD treatment, providing valuable insights into strategies targeting Aβ aggregation and neurodegeneration in AD.
A hybrid approach to investigating major management factors for effective highway preventive maintenance
Authors: Zhao Na , Liu Yijuan , Chen Huihua 
Published: October 2024

AbstractTo resolve the problem of the low management capacity of highway preventive maintenance (HPM), this paper identified and evaluated the major HPM management factors to improve management effectiveness and achieve sustainable highway development. The study conducted a literature review and exploratory factor analysis (EFA) to identify the major HPM management factors. Social network analysis (SNA) was used to distinguish the degree of importance of these factors. A system dynamics (SD) model was developed to explore their patterns of influence. The research identified six dimensions of HPM management, including the management system, management resources, management cognition, management decisions, management technology, and external conditions, along with 26 major management factors. Moreover, information acquisition, system perfection, etc., are key factors; system execution, manager capability, etc., are hub factors; and route selection, machinery allocation, etc., are non-key factors. These factors have a positive impact on HPM management, leading to an upward trend in management effectiveness. The main innovation provided a hybrid and comprehensive approach to identify and evaluate the major management factors for effective HPM. This study can guide managers in developing effective HPM plans, allocating resources more efficiently, improving the overall quality of highway maintenance and forming a sustainable transportation system.
Detection of PIK3CA hotspot mutations in canine mammary tumors using droplet digital PCR: tissue validation and liquid biopsy feasibility
Authors: Seung Byung-Joon , Sur Jung-Hyang 
Published: October 2024

AbstractDomestic dogs (Canis lupus familiaris) serve as valuable translational models for human cancer research due to their biological similarities. Canine mammary tumors (CMTs), frequently diagnosed in female dogs, share various characteristics with human breast cancers. This study investigates the PIK3CA (H1047R) mutation in CMTs using droplet digital PCR (ddPCR) and explores the potential of liquid biopsy for non-invasive detection. We analyzed 80 formalin-fixed, paraffin-embedded (FFPE) CMT tissue samples and compared ddPCR results with next-generation sequencing (NGS) data, achieving high concordance. Plasma and serum samples were also assessed for mutation concordance with tissue results. Our findings indicate a higher frequency of the PIK3CA (H1047R) mutations in benign and grade I malignant CMTs compared to more aggressive malignancies. The ddPCR assay demonstrated high sensitivity and specificity, with plasma testing showing 78.6% sensitivity and 87.5% specificity, and serum testing showing 66.7% sensitivity and 90.0% specificity. These results highlight the viability of liquid biopsy as a minimally invasive method for monitoring PIK3CA mutations in canine patients. The study suggests that liquid biopsy techniques hold significant promise for improving the early detection and monitoring of canine cancers, warranting further research to refine these methods and explore their applications in canine cancer diagnostics and treatment.
Design of gas control lane of 9# coal seam in Wuhushan Mine based on layer layout optimization
Authors: Zhang Chun , Qian Xianju 
Published: October 2024

AbstractIn order to address the issue of gas over limit in the upper corner of the working face of the 9# coal seam in Wuhushan Mine, a series of theoretical and numerical simulation analyses were conducted to evaluate the optimal configuration for the gas control lane of the 9# coal seam. In accordance with the "O" circle theory and the lithology of the overlying rock strata of the 9# coal seam, the height range of the fallout zone and fissure zone in the working face mining area was determined by employing empirical formulas. The change rule and distribution characteristics of the porosity of the fissure zone and the fall zone in the mining area were analyzed based on the characteristics of rock movement and fall. The determination method was also provided. The numerical simulation software was employed to simulate and analyze the gas concentration field in the air-mining zone under conditions of no extraction and six distinct layer positions of the gas control lane. The optimal layer position of the gas control lane in the 9# coal seam was determined and subsequently implemented in the field. The results demonstrate that the overlying rock layer in the 9# coal seam exhibits a height range of 6.86 ~ 11.26 m, while the fissure zone displays a height range of 30.11 ~ 41.31 m. When the gas control road is situated in close proximity to the working face, the gas concentration field exhibits a markedly low concentration. When the distance between the gas control lane and the return airway of the working face is 20 m and the distance from the top of the coal seam is 20 m, the gas concentration in the upper corner and the return airway is 0.35% and 0.26%, respectively. These values are close to the lowest concentration observed in the layout scheme. Additionally, the gas extraction concentration and the pure volume of the gas control lane are 23.7% and 38.3 m3 min−1, respectively. These values represent the highest concentrations observed in the various layout schemes. The application of the gas management lane in the field, based on the numerical simulation results, demonstrated a successful extraction effect, which was consistent with the numerical simulation results. This effectively managed the issue of an over-limit of gas in the upper corner of the working face of the 9# coal seam.
The effects of RT-qPCR standards on reproducibility and comparability in monitoring SARS-CoV-2 levels in wastewater
Authors: Juutinen Aapo , Tiwari Ananda , Hokajärvi Anna-Maria , Luomala Oskari , Kolehmainen Aleksi , Nurmi Eveliina , Salmivirta Elisa , Pitkänen Tarja , Lipponen Anssi 
Published: October 2024

AbstractReverse transcription-quantitative PCR (RT-qPCR) is widely used for monitoring viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in wastewater. Various materials, including plasmid DNA, synthetic nucleic acids, PCR amplicons, genomic DNA, and cDNA, are currently used for SARS-CoV-2 quantification by generating standard curves. We assessed three common standards on quantifying SARS-CoV-2 RNA across nine wastewater treatment plants in Finland, as part of the national wastewater surveillance effort. We pairwise compared RT-qPCR results from 148 wastewater samples, using both IDT (#10006625, IDT, USA) and CODEX standards (#SC2-RNAC-1100, CODEX DNA), and 179 samples using both IDT and EURM019 standards (#EURM-019, European Commission, Joint Research Centre) in our assessment. Amongst the tested standards, the CODEX standard consistently yielded more stable results than either the IDT or EURM019 standards. We found that SARS-CoV-2 levels were higher with the IDT standard (4.36 Log10 GC/100 mL) compared to the CODEX standard (4.05 Log10 GC/100 mL). Similarly, quantification using the IDT standard was higher (5.27 Log10 GC/100 mL) than values obtained with the EURM019 (4.81 Log10 GC/100 mL). SARS-CoV-2 RNA quantified with IDT and CODEX standards exhibited stronger concordance (Spearman’s correlation rho median of 0.79) compared to those quantified with IDT and EURM019 standards (rho median of 0.59). This study highlights the significant impact of standard material selection on SARS-CoV-2 RNA quantification, emphasizing the need for harmonization in standard material.
A high voltage gain solid-state transformer for integration of renewable energy and AC sources
Authors: Hashemzadeh Seyed Majid , Al-Hitmi Mohammed A. , Islam Shirazul , Iqbal Atif , Aghaei Hadi , Hosseini Seyed Hossein , Babaei Ebrahim 
Published: October 2024

AbstractThis paper introduces a novel high-voltage gain topology for a solid-state transformer, integrating a DC-DC converter and dual active bridge converters. The proposed design features three DC links operating at different voltage levels. The first DC link connects to a single-switch high step-up DC-DC converter, while the second DC link interfaces with an AC source via a rectifier, allowing the use of both DC and AC inputs. A high-frequency transformer ensures galvanic isolation between the sources and the third DC link. The DC-DC converter employs coupled inductors and voltage multiplier cells, offering distinct advantages such as a high voltage gain, reduced voltage stress on semiconductors, and minimized current ripple. These features make the topology highly suitable for transferring power from renewable energy sources, such as photovoltaic panels, to a high-voltage DC link in microgrid or nanogrid applications. The novelty lies in the combination of multiple voltage levels, high-frequency isolation, and the ability to handle both DC and AC inputs efficiently. An experimental prototype, delivering 620 W with a 25 V DC input and 110 V AC input, is built, and the results validate the converter’s effectiveness.
Grating-based phase-contrast computed tomography for breast tissue at an inverse compton source
Authors: Berthe Daniel , Heck Lisa , Resch Sandra , Dierolf Martin , Brantl Johannes , Günther Benedikt , Petrich Christian , Achterhold Klaus , Pfeiffer Franz , Grandl Susanne , Hellerhoff Karin , Herzen Julia 
Published: October 2024

AbstractThe introduction of mammography screening programs has significantly reduced breast cancer mortality rates. Nevertheless, some lesions remain undetected, especially in dense breast tissue. Studies have shown that phase-contrast imaging can improve breast cancer diagnosis by increasing soft tissue contrast. Furthermore, grating-based phase-contrast imaging enables the simultaneous acquisition of absorption, phase-contrast, and scattering, so-called dark-field images. The latter allows the classification of microcalcifications. In addition, breast computed tomography (BCT) systems can identify and discriminate overlapping but clinically relevant structures. This study investigates the benefit of combining grating-based phase-contrast with BCT. We explore the potential of grating-based phase-contrast breast computed tomography (gbpc-BCT) with a breast phantom and a freshly dissected fibroadenoma. Improved image contrast could be achieved with radiation doses comparable to those used in clinical BCT.
Identification of diagnostic markers pyrodeath-related genes in non-alcoholic fatty liver disease based on machine learning and experiment validation
Authors: Lei Liping , Li Jixue , Liu Zirui , Zhang Dongdong , Liu Zihan , Wang Qing , Gao Yi , Mo Biwen , Li Jiangfa 
Published: October 2024

AbstractNon-alcoholic fatty liver disease (NAFLD) poses a global health challenge. While pyroptosis is implicated in various diseases, its specific involvement in NAFLD remains unclear. Thus, our study aims to elucidate the role and mechanisms of pyroptosis in NAFLD. Utilizing data from the Gene Expression Omnibus (GEO) database, we analyzed the expression levels of pyroptosis-related genes (PRGs) in NAFLD and normal tissues using the R data package. We investigated protein interactions, correlations, and functional enrichment of these genes. Key genes were identified employing multiple machine learning techniques. Immunoinfiltration analyses were conducted to discern differences in immune cell populations between NAFLD patients and controls. Key gene expression was validated using a cell model. Analysis of GEO datasets, comprising 206 NAFLD samples and 10 controls, revealed two key PRGs (TIRAP, and GSDMD). Combining these genes yielded an area under the curve (AUC) of 0.996 for diagnosing NAFLD. In an external dataset, the AUC for the two key genes was 0.825. Nomogram, decision curve, and calibration curve analyses further validated their diagnostic efficacy. These genes were implicated in multiple pathways associated with NAFLD progression. Immunoinfiltration analysis showed significantly lower numbers of various immune cell types in NAFLD patient samples compared to controls. Single sample gene set enrichment analysis (ssGSEA) was employed to assess the immune microenvironment. Finally, the expression of the two key genes was validated in cell NAFLD model using qRT-PCR. We developed a prognostic model for NAFLD based on two PRGs, demonstrating robust predictive efficacy. Our findings enhance the understanding of pyroptosis in NAFLD and suggest potential avenues for therapeutic exploration.