bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel dual-band filter based on single-cavity CTSRR-loaded triangular substrate-integrated waveguide
Authors: Geng Q. F. , Guo H. J. , Zhu Y. Y. , Huang W. , Deng S. S. , Yang T. 
Published: May 2019

AbstractIn this paper, a novel single-cavity triangular substrate-integrated waveguide (TSIW) dual-band filter loading a complementary triangular split ring resonator (CTSRR) is proposed, which has three transmission zeros (TZs) in the stopband in total. The dual-band response is achieved by the CTSRR and the degenerate modes of the TSIW cavity. In order to control the TZs, we propose two adjustment techniques, shift feeding technique and adding via perturbation. In addition, the CTSRR etched on the surface can produce a new TZ in the upper first-passband. Finally, a dual-band filter with three TZs is simulated, fabricated, and measured. There is a good agreement between the simulated results and measured ones.
Compositional Constraints on the North Polar Cap of Mars from Gravity and Topography
Authors: Ojha Lujendra , Nerozzi Stefano , Lewis Kevin 
Published: May 2019

AbstractThe polar ice caps are the largest reservoir of water ice on Mars. The north polar ice cap is composed of the ice‐rich north polar layered deposit (NPLD) and a lower‐lying, silicate‐rich basal unit (BU). Together, these units represent a record of Martian climate history throughout the Amazonian period. Here we place a bulk compositional constraint on the BU by modeling its gravity signature in both spatial and spectral domains using two independent approaches. We find the density of the BU to be
kg/m3, suggesting that it may contain 55 + 25% water ice. We estimate that the BU contains ~1.5‐m global equivalent layer of water making it one of the largest reservoir of water‐ice on Mars. Our compositional constraint suggests that the north pole of Mars was not only a cold trap for ices but also an aeolian trap for silicates during the Amazonian period.
50 years of scanning electron microscopy of bone—a comprehensive overview of the important discoveries made and insights gained into bone material properties in health, disease, and taphonomy
Authors: Shah Furqan A. , Ruscsák Krisztina , Palmquist Anders 
Published: May 2019

AbstractBone is an architecturally complex system that constantly undergoes structural and functional optimisation through renewal and repair. The scanning electron microscope (SEM) is among the most frequently used instruments for examining bone. It offers the key advantage of very high spatial resolution coupled with a large depth of field and wide field of view. Interactions between incident electrons and atoms on the sample surface generate backscattered electrons, secondary electrons, and various other signals including X-rays that relay compositional and topographical information. Through selective removal or preservation of specific tissue components (organic, inorganic, cellular, vascular), their individual contribution(s) to the overall functional competence can be elucidated. With few restrictions on sample geometry and a variety of applicable sample-processing routes, a given sample may be conveniently adapted for multiple analytical methods. While a conventional SEM operates at high vacuum conditions that demand clean, dry, and electrically conductive samples, non-conductive materials (e.g., bone) can be imaged without significant modification from the natural state using an environmental scanning electron microscope. This review highlights important insights gained into bone microstructure and pathophysiology, bone response to implanted biomaterials, elemental analysis, SEM in paleoarchaeology, 3D imaging using focused ion beam techniques, correlative microscopy and in situ experiments. The capacity to image seamlessly across multiple length scales within the meso-micro-nano-continuum, the SEM lends itself to many unique and diverse applications, which attest to the versatility and user-friendly nature of this instrument for studying bone. Significant technological developments are anticipated for analysing bone using the SEM.
Combinatorial recognition of clustered RNA elements by the multidomain RNA-binding protein IMP3
Authors: Schneider Tim , Hung Lee-Hsueh , Aziz Masood , Wilmen Anna , Thaum Stephanie , Wagner Jacqueline , Janowski Robert , Müller Simon , Schreiner Silke , Friedhoff Peter , Hüttelmaier Stefan , Niessing Dierk , Sattler Michael , Schlundt Andreas , Bindereif Albrecht 
Published: May 2019

AbstractHow multidomain RNA-binding proteins recognize their specific target sequences, based on a combinatorial code, represents a fundamental unsolved question and has not been studied systematically so far. Here we focus on a prototypical multidomain RNA-binding protein, IMP3 (also called IGF2BP3), which contains six RNA-binding domains (RBDs): four KH and two RRM domains. We establish an integrative systematic strategy, combining single-domain-resolved SELEX-seq, motif-spacing analyses, in vivo iCLIP, functional validation assays, and structural biology. This approach identifies the RNA-binding specificity and RNP topology of IMP3, involving all six RBDs and a cluster of up to five distinct and appropriately spaced CA-rich and GGC-core RNA elements, covering a >100 nucleotide-long target RNA region. Our generally applicable approach explains both specificity and flexibility of IMP3-RNA recognition, allows the prediction of IMP3 targets, and provides a paradigm for the function of multivalent interactions with multidomain RNA-binding proteins in gene regulation.
Killer-like receptors and GPR56 progressive expression defines cytokine production of human CD4+ memory T cells
Authors: Truong Kim-Long , Schlickeiser Stephan , Vogt Katrin , Boës David , Stanko Katarina , Appelt Christine , Streitz Mathias , Grütz Gerald , Stobutzki Nadja , Meisel Christian , Iwert Christina , Tomiuk Stefan , Polansky Julia K. , Pascher Andreas , Babel Nina , Stervbo Ulrik , Sauer Igor , Gerlach Undine , Sawitzki Birgit 
Published: May 2019

AbstractAll memory T cells mount an accelerated response on antigen reencounter, but significant functional heterogeneity is present within the respective memory T-cell subsets as defined by CCR7 and CD45RA expression, thereby warranting further stratification. Here we show that several surface markers, including KLRB1, KLRG1, GPR56, and KLRF1, help define low, high, or exhausted cytokine producers within human peripheral and intrahepatic CD4+ memory T-cell populations. Highest simultaneous production of TNF and IFN-γ is observed in KLRB1+KLRG1+GPR56+ CD4 T cells. By contrast, KLRF1 expression is associated with T-cell exhaustion and reduced TNF/IFN-γ production. Lastly, TCRβ repertoire analysis and in vitro differentiation support a regulated, progressive expression for these markers during CD4+ memory T-cell differentiation. Our results thus help refine the classification of human memory T cells to provide insights on inflammatory disease progression and immunotherapy development.
Form vision from melanopsin in humans
Authors: Allen Annette E. , Martial Franck P. , Lucas Robert J. 
Published: May 2019

AbstractDetection and discrimination of spatial patterns is thought to originate with photoreception by rods and cones. Here, we investigated whether the inner-retinal photoreceptor melanopsin could represent a third origin for form vision. We developed a 4-primary visual display capable of presenting patterns differing in contrast for melanopsin vs cones, and generated spectrally distinct stimuli that were indistinguishable for cones (metamers) but presented contrast for melanopsin. Healthy observers could detect sinusoidal gratings formed by these metamers when presented in the peripheral retina at low spatial (≤0.8 cpd) and temporal (≤0.45 Hz) frequencies, and Michelson contrasts ≥14% for melanopsin. Metameric gratings became invisible at lower light levels (<1013 melanopsin photons cm−2 sr−1 s−1) when rods are more active. The addition of metameric increases in melanopsin contrast altered appearance of greyscale representations of coarse gratings and a range of everyday images. These data identify melanopsin as a new potential origin for aspects of spatial vision in humans.
Power generation from the interaction of a liquid droplet and a liquid membrane
Authors: Nie Jinhui , Wang Ziming , Ren Zewei , Li Shuyao , Chen Xiangyu , Lin Wang Zhong 
Published: May 2019

AbstractTriboelectric nanogenerators are an energy harvesting technology that relies on the coupling effects of contact electrification and electrostatic induction between two solids or a liquid and a solid. Here, we present a triboelectric nanogenerator that can work based on the interaction between two pure liquids. A liquid–liquid triboelectric nanogenerator is achieved by passing a liquid droplet through a freely suspended liquid membrane. We investigate two kinds of liquid membranes: a grounded membrane and a pre-charged membrane. The falling of a droplet (about 40 μL) can generate a peak power of 137.4 nW by passing through a pre-charged membrane. Moreover, this membrane electrode can also remove and collect electrostatic charges from solid objects, indicating a permeable sensor or charge filter for electronic applications. The liquid–liquid triboelectric nanogenerator can harvest mechanical energy without changing the object motion and it can work for many targets, including raindrops, irrigation currents, microfluidics, and tiny particles.
Double-shelled hollow rods assembled from nitrogen/sulfur-codoped carbon coated indium oxide nanoparticles as excellent photocatalysts
Authors: Sun Liming , Li Rong , Zhan Wenwen , Yuan Yusheng , Wang Xiaojun , Han Xiguang , Zhao Yanli 
Published: May 2019

AbstractExcellent catalytic activity, high stability and easy recovery are three key elements for fabricating efficient photocatalysts, while developing a simple method to fabricate such photocatalysts with these three features at the same time is highly challenging. In this study, we successfully synthesized double-shelled hollow rods (DHR) assembled by nitrogen (N) and sulfur (S)-codoped carbon coated indium(III) oxide (In2O3) ultra-small nanoparticles (N,S-C/In2O3 DHR). N,S-C/In2O3 DHR exhibits remarkable photocatalytic activity, high stability and easy recovery for oxidative hydroxylation reaction of arylboronic acid substrates. The catalyst recovery and surface area were well balanced through improved light harvesting, contributed by concurrently enhancing the reflection on the outer porous shell and the diffraction in the inside double-shelled hollow structure, and increased separation rate of photogenerated carriers. Photocatalytic mechanism was investigated to identify the main reactive species in the catalytic reactions. The electron separation and transfer pathway via N,S-codoped graphite/In2O3 interface was revealed by theoretical calculations.
Predicting protein-ligand interactions based on bow-pharmacological space and Bayesian additive regression trees
Authors: Li Li , Koh Ching Chiek , Reker Daniel , Brown J. B. , Wang Haishuai , Lee Nicholas Keone , Liow Hien-haw , Dai Hao , Fan Huai-Meng , Chen Luonan , Wei Dong-Qing 
Published: May 2019

AbstractIdentifying potential protein-ligand interactions is central to the field of drug discovery as it facilitates the identification of potential novel drug leads, contributes to advancement from hits to leads, predicts potential off-target explanations for side effects of approved drugs or candidates, as well as de-orphans phenotypic hits. For the rapid identification of protein-ligand interactions, we here present a novel chemogenomics algorithm for the prediction of protein-ligand interactions using a new machine learning approach and novel class of descriptor. The algorithm applies Bayesian Additive Regression Trees (BART) on a newly proposed proteochemical space, termed the bow-pharmacological space. The space spans three distinctive sub-spaces that cover the protein space, the ligand space, and the interaction space. Thereby, the model extends the scope of classical target prediction or chemogenomic modelling that relies on one or two of these subspaces. Our model demonstrated excellent prediction power, reaching accuracies of up to 94.5–98.4% when evaluated on four human target datasets constituting enzymes, nuclear receptors, ion channels, and G-protein-coupled receptors . BART provided a reliable probabilistic description of the likelihood of interaction between proteins and ligands, which can be used in the prioritization of assays to be performed in both discovery and vigilance phases of small molecule development.
Interaction of SNARE Mimetic Peptides with Lipid bilayers: Effects of Secondary Structure, Bilayer Composition and Lipid Anchoring
Authors: Wagle Swapnil , Georgiev Vasil N. , Robinson Tom , Dimova Rumiana , Lipowsky Reinhard , Grafmüller Andrea 
Published: May 2019

AbstractThe coiled-coil forming peptides ‘K’ enriched in lysine and ‘E’ enriched in glutamic acid have been used as a minimal SNARE mimetic system for membrane fusion. Here we describe atomistic molecular dynamics simulations to characterize the interactions of these peptides with lipid bilayers for two different compositions. For neutral phosphatidylcholine (PC)/phosphatidylethanolamine (PE) bilayers the peptides experience a strong repulsive barrier against adsorption, also observed in potential of mean force (PMF) profiles calculated with umbrella sampling. Forpeptide K, a minimum of −12kBTin the PMF provides an upper bound for the binding free energy whereas no stable membrane bound state could be observed forpeptide E. In contrast, the electrostatic interactions with negatively charged phosphatidylglycerol (PG) lipids lead to fast adsorption of both peptides at the head-water interface. Experimental data using fluorescently labeled peptides confirm the stronger binding to PG containing bilayers. Lipid anchors have little effect on the peptide-bilayer interactions or peptide structure, when the peptide also binds to the bilayer in the absence of a lipid anchor. Forpeptide E, which does not bind to the PC bilayer without a lipid anchor, the presence of such an anchor strengthens the electrostatic interactions between the charged side chains and the zwitterionic head-groups and leads to a stabilization of the peptide’s helical fold by the membrane.
Multiplexed detection of RNA using MERFISH and branched DNA amplification
Authors: Xia Chenglong , Babcock Hazen P. , Moffitt Jeffrey R. , Zhuang Xiaowei 
Published: May 2019

AbstractMultiplexed error-robust fluorescence in situ hybridization (MERFISH) allows simultaneous imaging of numerous RNA species in their native cellular environment and hence spatially resolved single-cell transcriptomic measurements. However, the relatively modest brightness of signals from single RNA molecules can become limiting in a number of applications, such as increasing the imaging throughput, imaging shorter RNAs, and imaging samples with high degrees of background, such as some tissue samples. Here, we report a branched DNA (bDNA) amplification approach for MERFISH measurements. This approach produces a drastic signal increase in RNA FISH samples without increasing the fluorescent spot size for individual RNAs or increasing the variation in brightness from spot to spot, properties that are important for MERFISH imaging. Using this amplification approach in combination with MERFISH, we demonstrated RNA imaging and profiling with a near 100% detection efficiency. We further demonstrated that signal amplification improves MERFISH performance when fewer FISH probes are used for each RNA species, which should allow shorter RNAs to be imaged. We anticipate that the combination of bDNA amplification with MERFISH should facilitate many other applications and extend the range of biological questions that can be addressed by this technique in both cell culture and tissues.
Field evaluation of synthetic and neem-derived alternative insecticides in developing action thresholds against cauliflower pests
Authors: Shah Farhan Mahmood , Razaq Muhammad , Ali Qasim , Shad Sarfraz Ali , Aslam Muhammad , Hardy Ian C. W. 
Published: May 2019

AbstractSynthetic chemical pesticides can enhance crop yields but also have undesired effects. Alternative ‘botanical insecticides’ may also have non-target effects on pollinators and biocontrol services. Employing action thresholds (ATs) can reduce pesticide (whether synthetic or botanical) use compared to fixed-interval applications. Here the azadirachtin-based botanical formulation NeemAzal and a neem seed extract (NSE) were evaluated in field spraying trials alongside commonly-used synthetics (Voliam Flexi [chlorentraniliprole plus thiamethoxam] and imidacloprid) in developing ATs for the regular and cosmopolitan cauliflower pests Brevicoryne brassicae, Plutella xylostella and Spodoptera litura. We considered the size of the S. litura larvae infesting the crop in order to derive ATs. ATs per plant were higher for NeemAzal (0.55 larvae for P. xylostella and 3 larvae for large-sized S. litura) than for Voliam Flexi (0.30 larvae for P. xylostella and 0.80 larvae for S. litura) but were similar for B. brassicae (50 individuals). Higher ATs when using azadirachtin were associated with the diverse modes of action of botanicals, for instance NeemAzal and NSE deterred oviposition of S. litura. Although the exact values of ATs are likely to have regional limits, our approach can be applied for determining ATs against common lepidopteran and aphid pests in many other vegetable crop agro-ecosystems.
Differential Immune Activation in Fetal Macrophage Populations
Authors: Lakhdari Omar , Yamamura Asami , Hernandez Gilberto E. , Anderson Kathryn K. , Lund Sean J. , Oppong-Nonterah Gertrude O. , Hoffman Hal M. , Prince Lawrence S. 
Published: May 2019

AbstractDistinct macrophage subsets populate the developing embryo and fetus in distinct waves. However little is known about the functional differences between in utero macrophage populations or how they might contribute to fetal and neonatal immunity. Here we tested the innate immune response of mouse macrophages derived from the embryonic yolk sac and from fetal liver. When isolated from liver or lung, CD11bHI fetal liver derived macrophages responded to the TLR4 agonist LPS by expressing and releasing inflammatory cytokines. However F4/80HI macrophages from the yolk sac did not respond to LPS treatment. While differences in TLR4 expression did not appear to explain these data, F4/80HI macrophages had much lower NLRP3 inflammasome expression compared to CD11bHI macrophages. Gene expression profiling also demonstrated LPS-induced expression of inflammatory genes in CD11bHI macrophages, but not in F4/80HI cells. Genes expressed in LPS-treated CD11bHI macrophages were more likely to contain predicted NF-κB binding sites in their promoter regions. Our data show that CD11bHI macrophages derived from fetal liver are the major pro-inflammatory cells in the developing fetus. These findings could have important implications in better understanding the fetal inflammatory response and the unique features of neonatal immunity.
Preliminary design of a new degradable medical device to prevent the formation and recurrence of intrauterine adhesions
Authors: Leprince Salome , Huberlant Stéphanie , Allegre Lucie , Warembourg Sophie , Leteuff Isabelle , Bethry Audrey , Paniagua Cedric , Taillades Hubert , De Tayrac Renaud , Coudane Jean , Letouzey Vincent , Garric Xavier 
Published: May 2019

AbstractIntrauterine adhesions lead to partial or complete obliteration of the uterine cavity and have life-changing consequences for women. The leading cause of adhesions is believed to be loss of stroma resulting from trauma to the endometrium after surgery. Adhesions are formed when lost stroma is replaced by fibrous tissue that join the uterine walls. Few effective intrauterine anti-adhesion barriers for gynecological surgery exist. We designed a degradable anti-adhesion medical device prototype to prevent adhesion formation and recurrence and restore uterine morphology. We focused on ideal degradation time for complete uterine re-epithelialization for optimal anti-adhesion effect and clinical usability. We developed a triblock copolymer prototype [poly(lactide) combined with high molecular mass poly(ethylene oxide)]. Comparative pre-clinical studies demonstrated in vivo anti-adhesion efficacy. Ease of introduction and optimal deployment in a human uterus confirmed clinical usability. This article provides preliminary data to develop an intrauterine medical device and conduct a clinical trial.
Scientific Backgrounds for the Timing of Acute Care Surgery
Authors: Eschmann David , Köck Manuela , Bludau Frederic , Obertacke Udo 
Published: May 2019

AbstractThe correct timing of urgent surgery in the field of orthopedic and trauma surgery is under constant discussion. The authors of this review like to present a scientific based recommendation for the timing of acute care surgery using the TACS-classification for the description of urgency. The timing and priority of the indicated procedure is deduced only from the expected mortality and disability caused by a potential delay. A proposal for a nomenclature is given to be integrated in the clinical practice and to be completed.
Fibroid Expulsion after Uterine Artery Embolization
Authors: Ochmanek Emily , Brown Matthew A. , Rochon Paul J. 
Published: May 2019

AbstractUterine artery embolization (UAE) is a ubiquitous procedure, and a broadly recognized alternative to surgical interventions for symptomatic leiomyomata when uterine preservation is desired. Aside from postembolization syndrome (typically considered an expected feature of recovery), the most frequently described complications are temporary or permanent amenorrhea and lingering vaginal discharge. Less frequently described complications include fibroid expulsion (FE), protracted or refractory pain, infection, urinary retention, and access-related injuries. Reported rates of transcervical FE vary in the literature from 3 to 50% but are most often quoted to be around 5 to 15%. Certain features predispose a patient to FE, including size and location of the tumor, with pedunculated submucosal, submucosal, and transmural lesions considered to be “high risk.” While the optimal management of FE has not been definitively determined, high rates of nonoperative management of FE are noted in the literature. This article describes a case in which a fibroid was expulsed following UAE, as well as the management of the complication. A literature review and recommendations for the management of FE is also given.