bluuebunny/crossref_metadata_2025_split huggingface dataset:
COMPARATIVE ANTI-DIABETIC EFFECTS OF ETHANOL EXTRACTS FROM LEAVES, SEEDS and PODS OF Moringa oleifera ON ALLOXAN INDUCED DIABETIC RATS
Authors: Adebayo Kingsley O. , Owolabi M. A. , Khan M. E. 
Published: December 2024

The anti-diabetic activity of 30 % ethanol extract of seeds, pods, leaves of Moringa oleifera was evaluated on induced diabetic rats for 4 weeks. After the treatment with extracts, the animals were sacrificed by cervical dislocation for the histopathological examination of their pancreas. Saponin and volatile oil were high in pod extract whereas high alkaloids, flavonoids, flavanols and anthraquinones were detected in all extracts. For bioassay, the animals were grouped into A, B, C, D, E, and F corresponding to treatment with 200 mg leave extract per kg body weight, 4 mL seed extract per kg body weight, 300 mg pod extract per kg body weight, 1.0 mL normal saline (diabetic control), 10 mg of metformin per kg body weight, 1.0 mL normal saline (Normal control) respectively. At the end of the treatment, it was found that 300 mg pod extract per kg body weight reduced the blood glucose level from 492.80 mg/dL to 128.30 mg/dL while 200 mg of leave extract per kg body weight reduced blood glucose level from 423.00 to 212.30 mg/dL. It was found that the antihyperglycaemic activity of 300 mg/kg pod extract is comparable to the standard drug. Also the observed activity was more for 300 mg/kg pod extract than 200 mg/kg leave extracts. Moreover at p > 0.005, there is a statistically significant difference between the blood glucose of the animals before and after they were treated for 28 days with both pod and leave extracts. Both leaves and pod extracts indicated antidiabetic activity,...
Penerapan Metode Dekomposisi dan Holt Winters Exponential Smoothing dalam Peramalan Penjualan Tambah Daya (Studi Kasus: PT PLN (Persero) ULP Petung)
Authors: Amelia Mila Yusi , Nugraheni Kartika 
Published: December 2024

Pada tanggal 15 Januari 2024 diadakan siaran pers oleh Kementerian Energi dan Sumber Daya. Berdasarkan artikel siaran pers tersebut dikatakan bahwa konsumsi listrik perkapita Indonesia terus meningkat sejak tahun 2017. PLN (Perusahaan Listrik Negara) menyediakan tenaga listrik dengan memberikan jasa pelayanan kepada masyarakat dengan menyelenggarakan bidang produksi transmisi dan distribusi tenaga listrik. PLN melakukan pekerjaan di bidang penyambungan listrik seperti tambah daya. Seiring dengan meningkatnya konsumsi listrik masyarakat, meningkat pula kebutuhan akan daya listrik. Sehingga diperlukan adanya penambahan daya. Data jumlah penjualan tambah daya pada PT PLN (Persero) ULP Petung tahun 2022 hingga 2023 mengalami peningkatan maupun penurunan. Oleh karena itu, dilakukan peramalan penjualan tambah daya pada tahun 2024 untuk pertimbangan perusahaan dalam menentukan kebijakan. Pada penelitian ini dilakukan peramalan dengan menggunakan metode Dekomposisi dan metode Holt Winters Exponential Smoothing. Metode ini dipilih karena merupakan metode time series yang menggunakan data dari masa sebelumnya untuk memprediksi masa selanjutnya. Pada penelitian ini akan dibandingkan nilai kesalahan (error) dari kedua metode tersebut untuk memperoleh metode terbaik. Berdasarkan hasil perhitungan nilai peramalan dari kedua metode yang digunakan, nilai MAPE terkecil yang digunakan untuk melakukan peramalan jumlah tambah daya adalah metode Holt Winters Exponential Smoothing dengan nilai MAPE sebesar 18,297 yang berkategori baik.
Pengamanan Pesan Teks Pada Citra Digital Menggunakan Kombinasi Algoritma RSA-Quasigroup Cipher dan Metode LSB Pola Zig-zag
Authors: Lesnussa Yedija Asyer , Tomasouw Berny Pebo 
Published: December 2024

Keamanan data merupakan hal yang sangat penting dalam menjaga kerahasiaan informasi terutama informasi sensitif yang hanya boleh diketahui oleh pihak yang berhak saja. salah satu teknik pengamanan data yang sering digunakan adalah rsa dan LSB. RSA dilakukan untuk menyembunyikan konten dari suatu informasi dengan mengubah informasi tersebut menjadi sandi dengan menggunakan kunci. quasigroup cipher adalah salah satu algoritma kriptografi yang untuk memecahkannya sangat sulit, sehingga bila dikombinasikan dengan algoritma RSA akan meningkatkan tingkat keamanan pesan atau informasi. Steganografi bertujuan untuk menyembunyikan pesan rahasia (hiding message) atau tulisan rahasia (covered writing) sehingga pesan tersebut tidak terdeteksi oleh orang lain. salah satu metode dalam steganografi adalah Least Significant Bit (LSB). LSB merupakan metode steganografi yang bekerja dengan mengubah bit yang paling tidak signifikan. kombinasi RSA-quasigroup cipher dan metode LSB diharapkan dapat meningkatkan keamanan pesan menjadi lebih baik. Hal ini bisa terlihat dari nilai psn dari hasil perbandingan citra sebelum dan sesudah disisipi pesan. berdasarkan hasil analisis kualitas citra, diperoleh bahwa nilai psnr terkecil adalah 68.61 dan psnr rata-rata seluruh citra yaitu 62,89. hasil ini memperlihatkan bahwa kombinasi RSA-quasigroup cipher dan metode LSB pola zig-zag memiliki tingkat kemanan yang sangat baik.
Studi Kualitatif: Identifikasi Determinan Audit Report Lag Pada Kantor Akuntan Publik DSI Cabang Surabaya
Authors: Umi Nur Fryda Kusli Rochmah  , Acynthia Ayu Wilasittha  
Published: December 2024

Abstrak

Penelitian ini bertujuan agar dapat mengidentifikasi determinan serta pencegahan terkait fenomena audit report lag pada Kantor Akuntan Publik (KAP) DSI Cabang Surabaya dengan menggunakan pendekatan kualitatif. Audit report lag merujuk pada selang waktu antar akhir periode akuntansi hingga tanggal diperiliskannya laporan audit yang menjadi indikator penting dari efisiensi serta efektivitas proses audit. Wawancara kepada para auditor KAP Djoko, Sidik & Indra Cabang Surabaya yang berpengalaman dalam menangani kasus audit report lag menjadi sumber data yang primer dari penelitian ini. Melalui wawancara mendalam bersama auditor juga manajer di KAP DSI cabang Surabaya, studi ini mengungkap berbagai macam faktor utama yang berkaitan dengan audit report lag. Hasil penelitian mengindikasikan bahwa kapasitas dan beban kerja auditor, kompleksitas klien, perencanaan juga penjadwalan audit, serta koordinasi bersamaan klien merupakan determinan utama yang berkontribusi ke audit report lag. Dari temuan tersebut, terdapat beberapa saran praktis yang dapat dilakukan oleh auditor untuk meminimalisir audit report lagi antara lain optimalisasi distribusi beban kerja, peningkatan perencanaan juga penjadwalan audit, program pelatihan berkelanjutan, serta perbaikan komunikasi juga koordinasi bersamaan klien.

Kata Kunci: Audit Report Lag; Kantor Akuntan Publik; juga Kualitas Audit
Analisis Pengaruh Penerapan Green Accounting, Kinerja Lingkungan Dan Pengungkapan Corporate Social Responsibility Terhadap Return Saham
Authors: Sekar Arum Nurlatifah  , Annafi Indra Tama  
Published: December 2024

Abstrak
Studi ini bertujuan untuk memberikan informasi bagi para investor dan manajer perusahaan dalam memahami factor-faktor yang mempengaruhi tingkat pengembalian saham. Kajin ini juga menyoroti pentingnya strategi keberlanjutan dalam meningkatkan daya tarik investasi. Dalam pasar modal, PT Unilever Indonesia Tbk menghadapi penurunan harga saham sebesar 30,95% year-to-date. Untuk menghadapi situasi ini, perusahaan mengambil inisiatif strategi dengan mengedepankan upaya pelestarian ekosistem. Langkah ini diharapkan dapat memperbaiki minat investor dan mengoptimalkan tingkat return saham. Studi ini bertujuan untuk menyelidiki dampak penerapan green accounting, kinerja lingkungan serta pengungkapan corporate social responsibility terhadap retrun saham. Metodologi penelitian ini menggunakan uji asumsi klasik dan analisis regresi unbalanced data menggunakan bantuan software E-Views 12. Data yang dimanfaatkan dalam penelitian ini bersumber dari dokumentasi perusahaan dalam sektor consumer non-cyclical yang tercatat sebagai emiten di pasar modal Indonesia. Sampel sipilih menggunakan teknik purposive sampling mencakup periode 2020-2022, menghasilkan 31 sampel perusahaan sebagai subjek penelitian. Temuan studi ini menemukan bahwa penerapam green accounting dan kinerja lingkungan memiliki pengaru terhadap return saham. sedangkan pengungkapan corporate social responsibility tidak memiliki pengaruh terhadap return saham.
Kata kunci: green accounting; kinerja lingkungan; csr; return saham;
Cell-free supernatant of Lactobacillus gasseri 1A-TV shows a promising activity to eradicate carbapenem-resistant Klebsiella pneumoniae colonization
Authors: Vertillo Aluisio Gaia , Mezzatesta Maria Lina , Cafiso Viviana , Scuderi Renata , Stefani Stefania , Santagati Maria 
Published: December 2024

BackgroundThe use of beneficial bacteria like Lactobacillus spp. is a potential innovative approach to fight antibiotic-resistant pathogens. Klebsiella pneumoniae is one of the most concerning multi drug-resistant (MDR) pathogens, and its ability to colonize the human gut is considered to be the main reason for recurrent infections in critically ill patients.MethodsIn this study, Lactobacillus gasseri 1A-TV, already described for its probiotic activity, was characterized at the genomic level. Moreover, its cell-free supernatant (CFS) was tested for antimicrobial activity against extended-spectrum β-lactamase (ESBL)- and carbapenemase (KPC)-producing K. pneumoniae clinical isolates.ResultsWhole-genome sequencing showed that the L. gasseri 1A-TV genome was of 2,018,898 bp in size with 34.9% GC content, containing 1,937 putative protein coding sequences, 55 tRNA, and 4 rRNA detected by RAST and classified in 20 functional groups by Cluster of Orthologous Genes (COG). BAGEL4 (BActeriocin GEnome minimal tooL) and the antiSMASH 7.0 pipeline identified two bacteriocin biosynthetic gene clusters (BBGCs), namely, BBGC1 that comprises two class IIc bacteriocins including gassericin A-like bacteriocin, and BBGC2 carrying the class III bacteriocin helveticin J. Strikingly, 1A-TV CFS inhibited the growth of all K. pneumoniae isolates only after 8 h of incubation, showing a bactericidal effect at 24 h and interfering, even at lower concentrations, with the biofilm production of biofilm-producer strains independently of a bactericidal effect. NMR analysis of CFS identified and quantified several metabolites involved in carbohydrate metabolism and amino acid metabolism, and organic acids like ethanol, lactate, acetate, and succinate. Finally, in vitro assays of 1A-TV showed significant co-aggregation effects against carbapenem-resistant K. pneumoniae, namely, strains 1, 2, 3, and 7.ConclusionsOur findings highlight the antimicrobial activity of 1A-TV as a probiotic candidate or its CFS as a natural bioproduct active against MDR K. pneumoniae strains, underlining the importance of novel therapeutic strategies for prevention and control of ESBL- and carbapenemase-producing K. pneumoniae colonization.
Her, His, and their journey with endometriosis: a qualitative study
Authors: Shinan-Altman Shiri , Wertheimer Aya , Frankel Bat-El , Hamama-Raz Yaira 
Published: December 2024

BackgroundEndometriosis, impacting roughly 10% of reproductive-age women and girls globally, presents diagnostic challenges that can cause significant delays between symptom onset and medical confirmation. The aim of the current study was to explore the experience of women with endometriosis as well as that of their partners, from pre-diagnosis to diagnosis to post-diagnosis.MethodsIn-depth semi-structured interviews were conducted with 10 couples coping with endometriosis. Each partner was interviewed separately, and each interview was analyzed both individually and as part of a dyad, using the dyadic interview analysis method.ResultsThree main themes emerged: (i) “Relationship in the shadow of uncertainty”: Coping with health symptoms prior to the formal endometriosis diagnosis; (ii) Coping together or alone when receiving the endometriosis diagnosis; and (iii) “The day after the diagnosis”: Moving between adversity and growth.ConclusionsThe study's findings emphasize the importance of viewing the diagnosis from a dyadic perspective and comprehensively – that is, from pre-diagnosis to accepting the formal diagnosis to post-diagnosis. This journey can have a profound impact on both couple members, affecting their day-to-day functioning, communication, emotional and physical intimacy, and fertility.
Identification of SPP1+ macrophages as an immune suppressor in hepatocellular carcinoma using single-cell and bulk transcriptomics
Authors: Jin Han , Kim Woonghee , Yuan Meng , Li Xiangyu , Yang Hong , Li Mengzhen , Shi Mengnan , Turkez Hasan , Uhlen Mathias , Zhang Cheng , Mardinoglu Adil 
Published: December 2024

IntroductionMacrophages and T cells play crucial roles in liver physiology, but their functional diversity in hepatocellular carcinoma (HCC) remains largely unknown.MethodsTwo bulk RNA-sequencing (RNA-seq) cohorts for HCC were analyzed using gene co-expression network analysis. Key gene modules and networks were mapped to single-cell RNA-sequencing (scRNA-seq) data of HCC. Cell type fraction of bulk RNA-seq data was estimated by deconvolution approach using single-cell RNA-sequencing data as a reference. Survival analysis was carried out to estimate the prognosis of different immune cell types in bulk RNA-seq cohorts. Cell-cell interaction analysis was performed to identify potential links between immune cell types in HCC.ResultsIn this study, we analyzed RNA-seq data from two large-scale HCC cohorts, revealing a major and consensus gene co-expression cluster with significant implications for immunosuppression. Notably, these genes exhibited higher enrichment in liver macrophages than T cells, as confirmed by scRNA-seq data from HCC patients. Integrative analysis of bulk and single-cell RNA-seq data pinpointed SPP1+ macrophages as an unfavorable cell type, while VCAN+ macrophages, C1QA+ macrophages, and CD8+ T cells were associated with a more favorable prognosis for HCC patients. Subsequent scRNA-seq investigations and in vitro experiments elucidated that SPP1, predominantly secreted by SPP1+ macrophages, inhibits CD8+ T cell proliferation. Finally, targeting SPP1 in tumor-associated macrophages through inhibition led to a shift towards a favorable phenotype.DiscussionThis study underpins the potential of SPP1 as a translational target in immunotherapy for HCC.
Case report: Vedolizumab in Oral Crohn’s disease: the downsides of a gut-specific therapy for a multi-site disease
Authors: Harte Molly , Macken John , Zou Lifong , Fortune Farida 
Published: December 2024

IntroductionCrohn’s disease (CD) is a chronic inflammatory bowel disease which can affect any area of the gastrointestinal tract, including oral tissues. The complex nature of this disease demands interdisciplinary management, especially when both intestinal and oral manifestations are present.CaseThis report presents the case of a 28-year-old male patient with oral, ileo-caecal and peri-anal CD managed jointly between Gastroenterology and Oral Medicine. Treatment with vedolizumab, an α4β7 integrin with gut-selective anti-inflammatory activity, resulted in excellent ileo-caecal disease control, but was ineffective in controlling oral manifestations. The absence of MAdCAM-1 expression in oral tissues, necessary for vedolizumab’s mechanism, meant that the drug’s anti-inflammatory effects were limited to the gut. This limitation led to worsening oral symptoms, necessitating concomitant azathioprine therapy to manage oral inflammation.ConclusionMultidisciplinary collaboration is important when managing CD patients with both oral and gut involvement in CD. Clinicians should be aware that vedoluzimab may be beneficial for intestinal CD, but does not target inflammation in oral tissues due to its gut-specific action. Good knowledge of the pharmacology and mechanism of action of drugs prescribed can aid decision making when prescribing for this group of patients and can limit the need for polypharmacy, often associated with an increased adverse effect profile.
Reliability, biological variability, and accuracy of multi-frequency bioelectrical impedance analysis for measuring body composition components
Authors: Looney David P. , Schafer Erica A. , Chapman Christopher L. , Pryor Riana R. , Potter Adam W. , Roberts Brandon M. , Friedl Karl E. 
Published: December 2024

IntroductionBioelectrical impedance analysis (BIA) systems are gaining popularity for use in research and fitness assessments as the technology improves and becomes more affordable and easier to use. Multifrequency BIA (MF-BIA) may improve accuracy and precision using octopolar contacts for segmental analyses.PurposeEvaluate reliability, biological variability, and accuracy of component measures (total body water, mass, and composition) of commercially available MF-BIA system (InBody 770, Cerritos, California, USA).MethodsFourteen healthy military-age adults were assessed by MF-BIA in duplicate on five laboratory visits across 3 weeks (10 measures each). Participants were evaluated at the same time of day after refraining from strenuous exercise (&gt; 48 h), alcohol consumption (&gt; 24 h), and caffeine, nicotine, and food (&gt; 10 h). Systematic error (test–retest reliability) and biological variability (day-to-day reliability) were summarized by intraclass correlation coefficient (ICC) values determined for body mass (fat, fat-free, total) and body water (extracellular, intracellular, total). Body composition measurements derived from BIA on the second visit were also tested for accuracy compared to dual-energy x-ray absorptiometry (DXA).ResultsTest–retest reliability was very high for all measurements of whole-body water and mass (ICC ≥ 0.999) and high for regional body water and mass (ICC 0.973–1.000). Biological variability was observable with very minor differences between tests (same day) for total and regional body water (0.0–0.2 L) and total and regional body mass measurements (0.0–0.2 kg); while between day differences were slightly higher (0.0–0.5 L and 0.1–0.7 kg). Compared to DXA, the MF-BIA whole-body measures showed an offset in %BF (Bias −4.0 ± 2.8%; Standard error of the estimate (SEE), 2.6%), an overprediction for total body fat-free mass (Bias 2.8 ± 2.1 kg; SEE 2.2 kg) and an underprediction of total body fat mass (Bias −2.9 ± 2.0 kg; SEE 1.9 kg).ConclusionUnder controlled conditions with fit and healthy men and women, this MF-BIA system has high methodological reliability and demonstrates stable day-to-day measurements of major body composition components. Previously reported ~3% body fat offset compared to criterion methods was again confirmed. Precision of the InBody 770 shows consistency and supports further testing of this specific device as a new military standards method and suitability across a wider range of %BF.
In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells
Authors: Park Seokmuk , Park Han Woong , Seo Dae Bang , Yoo Dae Sung , Bae Seunghee 
Published: December 2024

BackgroundScalp hair plays a crucial role in social communication by expressing personal appearance and self-identity. Consequently, hair loss often leads to a perception of unattractiveness, negatively impacting an individual’s life and mental health. Currently, the use of Food and Drug Administration (FDA)-approved drugs for hair loss is associated with several side effects, highlighting the need for identifying new drug candidates, such as plant-derived phytochemicals, to overcome these issues.ObjectiveThis study investigated the hair growth-promoting effects of araliadiol, a polyacetylene compound found in plants such as Centella asiatica.MethodsWe employed an in vitro model comprising human hair follicle stem cells (HHFSCs) and human dermal papilla cells (HDPCs) to evaluate the hair growth-promoting effects of araliadiol. The proliferation-stimulating effects of araliadiol were assessed using water-soluble tetrazolium salt assay, adenosine triphosphate content assay, and crystal violet staining assay. In addition, we performed luciferase reporter assay, polymerase chain reaction analysis, cell fractionation, Western blot analysis, and enzyme-linked immunosorbent assay (ELISA) to elucidate the mechanism underlying the hair growth-inductive effects of araliadiol.ResultsAraliadiol exhibited both proliferation- and hair growth-promoting effects in HHFSCs and HDPCs. Specifically, it increased the protein expression of cyclin B1 and Ki67. In HHFSCs, it elevated the expression of hair growth-promoting factors, including CD34, vascular endothelial growth factor (VEGF), and angiopoietin-like 4. Similarly, araliadiol increased the expression of hair growth-inductive proteins such as fibroblast growth factor 7, VEGF, noggin, and insulin-like growth factor 1 in HDPCs. Subsequent Western blot analysis and ELISA using inhibitors such as GW9662 and SB202190 confirmed that these hair growth-promoting effects were dependent on the p38/PPAR-γ signaling in both HHFSCs and HDPCs.ConclusionAraliadiol promotes hair growth through the p38/PPAR-γ signaling pathway in human hair follicle cells. Therefore, araliadiol can be considered a novel drug candidate for the treatment of alopecia.
Computational studies for pre-evaluation of pharmacological profile of gut microbiota-produced gliclazide metabolites
Authors: Đanić Maja , Pavlović Nebojša , Zaklan Dragana , Stanimirov Bojan , Lazarević Slavica , Al-Salami Hani , Mikov Momir 
Published: December 2024

BackgroundGliclazide, a second-generation sulfonylurea derivative still widely used as a second-line treatment for type 2 diabetes mellitus, is well known to be subject to interindividual differences in bioavailability, leading to variations in therapeutic responses among patients. Distinct gut microbiota profiles among individuals are one of the most crucial yet commonly overlooked factors contributing to the variable bioavailability of numerous drugs. In light of the shift towards a more patient-centered approach in diabetes treatment, this study aimed to conduct a pharmacoinformatic analysis of gliclazide metabolites produced by gut microbiota and assess their docking potential with the SUR1 receptor to identify compounds with improved pharmacological profiles compared to the parent drug.MethodsTen potential gliclazide metabolites produced by the gut microbiota were screened for their pharmacological properties. Molecular docking analysis regarding SUR1 receptor was performed using Molegro Virtual Docker software. Drug-likeness properties were evaluated using DruLiTo software. Subsequently, the physicochemical and pharmacokinetic properties of gliclazide and its metabolites were determined by using VolSurf+ software package.ResultsAll studied metabolites exhibited better intrinsic solubility than gliclazide, which is of interest, considering that solubility is a limiting factor for its bioavailability. Based on the values of investigated molecular descriptors, hydroxylated metabolites M1-M6 showed the most pronounced polar and hydrophilic properties, which could significantly contribute to their in vivo solubility. Additionally, docking analysis revealed that four hydroxyl-metabolites (M1, M3, M4, and M5), although having a slightly poorer permeability through the Caco-2 cells compared to gliclazide, showed the highest binding affinity to the SUR1 receptor and exhibited the most suitable pharmacological properties.ConclusionIn silico study revealed that hydroxylated gut microbiota-produced gliclazide metabolites should be further investigated as potential drug candidates with improved characteristics compared to parent drug. Moreover, their part in the therapeutic effects of gliclazide should be additionally studied in vivo, in order to elucidate the role of gut microbiota in gliclazide pharmacology, namely from the perspective of personalized medicine.