bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of lncRNA DANCR promotes osteogenic differentiation of periodontal ligament stem cells
Authors: Wang Zhuo , Huang Yuanliang , Tan Luanjun 
Published: July 2019

Abstract
Backgrounds: Long non-coding RNAs (lncRNAs) have been widely known to have an appreciable effect in physiology and pathology. In tooth regeneration, periodontal ligament stem cells (PDLSCs) are regarded as a key effector, whereas, how lncRNA acts in the osteogenic differentiation of PDLSCs haven’t been completely understood. This study aims to find out the relationship between lncRNA DANCR and the proliferation and osteogenic differentiation of PDLSCs.
Results: Compared with the undifferentiated PDLSCs, the alizarin red staining level was higher in differentiated PDLSCs. And the expressions of osteogenic differentiation marker genes Runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and bone morphogenetic protein (BMP-2) were significantly increased in the differentiated PDLSCs. Furthermore, we noticed that comparing with control groups, the expression of LncRNA DANCR decreases markedly in osteogenically induced PDLSCs. DANCR promoted proliferation of PDLSCs, as evidenced by cell viability. Further investigation has proven that the downregulation of DANCR shows in the calcium sediment forming, alkaline phosphatase (ALP) activation and some osteogenic-related gene markers’ upregulation including Runx2, OCN and BMP-2, which finally results in the osteogenic differentiation of PDLSCs following the transfection and induction. Conversely, DANCR upregulation was shown to repress the osteogenic differentiation potential of PDLSCs.
Conclusions: The osteogenic differentiation of PDLSCs has proven to related to the down regulation of lncRNA DANCR. And this paper throws light on the effects of DANCR in the process of PDLSCs’ osteogenic differentiation.
Retinoic acid receptor-related orphan receptor α reduces lipid droplets by upregulating neutral cholesterol ester hydrolase 1 in macrophages
Authors: MATSUOKA Hiroshi , TOKUNAGA Riki , HOSODA Yuichiro , KATAYAMA Miyu , MIYA Kaoruko , SUMI Kento , OHISHI Ami , KAMISHIKIRYO Jun , SHIMA Akiho , MICHIHARA Akihiro 
Published: July 2019

Abstract
Background: Neutral cholesterol ester hydrolase 1 (NCEH1) catalyzes the hydrolysis of cholesterol ester (CE) in macrophages. Genetic ablation of NCEH1 promotes CE-laden macrophages and the development of atherosclerosis in mice. Dysregulation of NCEH1 levels is involved in the pathogenesis of multiple disorders, including metabolic diseases and atherosclerosis; however, relatively little is known regarding the mechanisms regulating NCEH1. Retinoic acid receptor-related orphan receptor α (RORα)-deficient mice exhibit several phenotypes indicative of aberrant lipid metabolism, including dyslipidemia and increased susceptibility to atherosclerosis. Results: In this study, inhibition of lipid droplet formation by RORα positively regulated NCEH1 expression in monocyte-derived macrophages. In mammals, the NCEH1 promoter region had putative RORα response elements (ROREs). Electrophoretic mobility shift, chromatin immunoprecipitation, and luciferase reporter assays showed that RORα bound and responded to the ROREs in human NCEH1. Moreover, NCEH1 was upregulated through RORα in a phorbol myristate acetate-dependent mechanism during macrophage differentiation from THP1 cells. siRNA-mediated knockdown of RORα significantly downregulated NCEH1 and inhibited lipid droplet formation in human hepatoma cells. In contrast, NCEH1 was induced by treatment with RORα agonists. Conclusion: These data strongly suggested that NCEH1 was a direct RORα target, defining potential new roles for RORα in inhibition of lipid droplet formation through NCEH1.
Accuracy of urine calprotectin for diagnosis of acute kidney injury in neonates
Authors: Khalesi Nasrin , Mohamadian Shima , Hooman Nakysa , Khodadost Mahmoud 
Published: July 2019

Abstract
Background: Urine calprotectin significantly rises in acute kidney injury (AKI) in adult and pediatrics. The aim of the present study was to investigate the accuracy of urine calprotectin as a diagnostic marker for (AKI) in neonates. Methods In this cross sectional study, we assessed urine calprotectin in 100 neonates, in which 80 of them had AKI and 20 were healthy. Random urine calprotectin measured by ELISA and then compared between two groups. We included neonates who had received at least 48 hour intra venous fluid and met our inclusion and exclusion criteria. . Receiver-operating characteristic (ROC) curve used to set a cut of point for urine calprotectin to predict AKI. The overall accuracy and Kappa coefficient was used for assess the agreement between two methods.. P value below 0.05 considered significant. Results: Urine calprotectin levels were not significantly higher in neonates with AKI than healthy ones (146.2 vs 142.4, p=0.1). The results showed an optimal cutoff value of 123.5 mg/dl for urine calprotectin with area under the curve of 0•515 with sensitivity, specificity, positive predictive value and negative predictive value of 77.5%,40%,83.7% and 30.7%, respectively. The overall accuracy was 70% and the Kappa agreement coefficient was 0.15 (P=0.11.). Conclusion: Although urine calprotectin level can predict the AKI, it is not accurate measure comparing the gold standard.
Assessing the Validity and Reliability of a Socio-Cognitive Determinants of Water Intake Questionnaire in Kidney Stones Patients
Authors: Mirzaei-Alavijeh Mehdi , Jalilian Farzad , Fatahi Halimeh , Babakhani Maryam , Solaimanizadeh Laleh 
Published: July 2019

Abstract
Background

Developing a valid and reliable questionnaire is an important step in field studies, which can help to tailor health-related behavior interventions. This study aimed at the psychometric analysis of the socio-cognitive determinants of water intake questionnaire behaviors among patients with kidney stones in the west of Iran.

Methods

Items were generated from interviews with kidney stones patients and experts. As well as, our items pool were developed from the questionnaires of socio-cognitive determinants related to the water intake. Internal consistency, face, content, and construct validities were tested. Data were analyzed by SPSS (ver. 20.0).

Results

Based on Eigen values of ≥ 1.00 and factor loadings of ≥ 0.40, five determinants were extracted. The calculated Kaiser–Meyer–Olkin (KMO) value was 0.697. The socio-cognitive determinants of water intake questionnaires were found to have acceptable internal consistency (Cronbach alpha of determinants between 0.65-0.85) and the theoretical assumptions for face, content, and construct validities were confirmed. Overall, the five studied socio-cognitive determinants explained 73.83% of the variance in the proposed model.

Conclusions

The socio-cognitive determinants of water intake questionnaire were revealed to have an acceptable psychometric evaluation. The questionnaire could be used to predict or explain water intake behavior in order to develop programs to increase water intake behavior among kidney stones patients.
Motor Intervention With And Without Nintendo® Wii For Children With Developmental Coordination Disorder: Protocol For A Randomized Clinical Trial
Authors: NETO JORGE Lopes CAVALCANTE , Steenbergen Bert , Tudella Eloisa 
Published: July 2019

Abstract
Background

Despite the benefits highlighted by motor interventions based on virtual reality for children with Developmental Coordination Disorder (DCD), there are still doubts whether these are greater than those obtained with conventional interventions due to the absence of systematized protocols and lack of evidence. Here we present a protocol to systematically compare the effects of two motor training programs (one Nintendo® Wii based and the other no-Wii motor activities) on the motor learning in children with DCD.

Methods/Design

Two intervention protocols (one based on Nintendo® Wii and the other no-Wii motor activities) will be carried out, with interventions occurring twice a week in 60-minute sessions, with a minimum of 12 and a maximum of 16 sessions per child.

The protocols were developed based on the domains of the Movement Assessment Battery for Children – Second Edition (MABC-2) (Manual dexterity, aiming & catching, balance), with two activities for each of the MABC -2 domains. The study will include children aged 7 to 10 with total MABC-2 score ≤16, and Developmental Coordination Disorder Questionnaire (DCDQ) score <46 (age of 7 years), score <55 (age group of 8 to 9 years and 11 months), or score <57 (age of 10 years) as scored by the parents. Children will be randomly allocated by draw in one of the two intervention protocols. MABC-2 and DCDQ will be applied before and after intervention to evaluate the effects of the interventions on motor performance and parents’ perception respectively. Motor learning will be assessed by means of the scores obtained in the games. Evaluators and therapists will be trained and blind about the data of the children in the study.
Efficiency of using different lining materials to protect groundwater from leakage of polluted streams
Authors: Abd-Elhamid Hany F. , Abdelaal Gamal M. , Abd-Elaty Isamil , Said Abdelrahman M. 
Published: July 2019

Abstract
Groundwater is one of the most important water sources but it is often not far away from pollution. One source of pollution is leakage from polluted streams such as open drains. The effect of open drains on groundwater quality has become an essential issue. This study aims to use different lining materials to minimize the seepage from open drains to protect groundwater. MODFLOW is used to investigate flow and contaminant transport and to evaluate efficiency of different lining materials. A hypothetical case study is used to assess different lining materials such as clay, bentonite, geomembranes and concrete. The results showed that decreasing the conductivities of lining materials reduced the extension of the contaminate. The extension of contaminants was reduced by 43, 89.6, 91.4 and 93% compared with the base case when drains were lined by clay, bentonite, geomembranes and concrete, respectively. Also, cost analysis of lining materials was done to detect the best lining material. Lining using geomembranes reduced contaminant extension at low cost compared with concrete, which reduced contaminant extension at double the cost. This reveals that the geomembranes represent the preferred material to protect groundwater from drain seepage due to its high durability and low cost compared with concrete.
The Kailash Ecovillage project converting human excreta into organic foodstuffs and sanitized compost using new international building codes for compost toilet and urine diversion systems
Authors: Ersson O. , King K. 
Published: July 2019

Abstract
Since March 2014, a sustainably focused community located on a 0.7 hectares site in Portland, Oregon, USA, has been undertaking an experimental composting toilet system modeled after the Water Efficiency and Sanitation Standard (WE-Stand) set out by the International Association of Plumbing and Mechanical Officials (IAPMO). This system collects urine and hot composts human excreta in a dry-composting toilet system for eventual use on the community's organic gardens. The system design reduces the need to access municipal water, sewer, and electrical infrastructure, enhancing emergency preparedness. It conserves an otherwise wasted nutrient flow, and safely produces a valuable compost. The system consists of urine collection vessels, multiple portable collection containers for excreta, toilet paper, and additive, and a compost processor. Urine diversion has allowed the community to reclaim nitrogen and other nutrients otherwise lost in conventional sewage systems, resulting in large savings of potable water and significant carbon sequestration via topsoil creation. Logs showed thermophilic compost temperatures. Compost and urine pathogen testing met American National Standards Institute and National Sanitation Foundation Standard 41 requirements.
Dynamic arrays in C ++ and minimax search problem
Authors: Alimov Timur Mansurovich , Alabdullakh Mokhamad Belal , Alkhuzaii Akhmed Khusham khusam , Kattavi Radzhikh Ziad 
Published: July 2019

The article presents the classical saddle-point problem in a two-dimensional array. Such tasks are highly demanded in geodesy, in the construction of topographic or gravimetric maps of the terrain, in the energy sector – in planning the load on electric or thermal networks. The program code of the proposed project is written within the framework of the structured approach in the C ++ language in the Visual Studio 2017 programming environment.
A Comprehensive Review on the Therapeutic Versatility of Imidazo [2,1-b]thiazoles
Authors: Shareef Mohd Adil , Khan Irfan , Babu Bathini Nagendra , Kamal Ahmed 
Published: July 2019

Background::
Imidazo[2,1-b]thiazole, a well-known fused five-membered hetrocycle is
one of the most promising and versatile moieties in the area of medicinal chemistry. Derivatives of
imidazo[2,1-b]thiazole have been investigated for the development of new derivatives that exhibit
diverse pharmacological activities. This fused heterocycle is also a part of a number of therapeutic
agents.

Objective::
To review the extensive pharmacological activities of imidazo[2,1-b]thiazole derivatives
and the new molecules developed between 2000-2018 and their usefulness.

Method::
Thorough literature review of all relevant papers and patents was conducted.

Conclusion::
The present review, covering a number of aspects, is expected to provide useful insights
in the design of imidazo[2,1-b]thiazole-based compounds and would inspire the medicinal
chemists for a comprehensive and target-oriented information to achieve a major breakthrough in
the development of clinically viable candidates.
Genetic Polymorphisms of Pharmacogenomic VIP Variants in the Circassian Subpopulation from Jordan
Authors: AL-Eitan Laith N. , Mohammad Namarg N. , Al-Maqableh Haneen W. , Hakooz Nancy M. , Dajani Rana B. 
Published: July 2019

Background:
It has been suggested that genetic variation within candidate pharmacogenes contributes to
the differences in drug safety and efficacy as well as risk of adverse drug reactions among different ethnic groups.
Illustrating the polymorphic distribution of Very Important Pharmacogenes (VIPs) in various ethnic groups will
contribute to the development of personalized medicine for those populations.

Objective:
The present study aimed to identify the polymorphic distribution of VIPs in the Circassian subpopulation
of Jordan and compare their allele frequencies with those of other populations.

Methods:
A total of 130 healthy and unrelated Circassian adults from Jordan were randomly recruited and genotyped
for eleven VIP variants within the thiopurine S-methyltransferase (TPMT), ATP-binding cassette, sub-family B,
member 1 (ABCB1), and vitamin D receptor (VDR) genes via Sequenom’s MassARRAY® genotyping platform
(iPLEX GOLD).

Results:
Our data on the allelic frequencies of the investigated VIP variants were compared to those of 18 other
populations, comprising 11 HapMap populations, 6 Exome Aggregation Consortium populations, and the Chechen-
Jordanian population from Jordan. Circassian-Jordanians were found to most resemble the African, Chechen-
Jordanian, European (Finnish), European (non-Finnish), and South-Asian populations.

Conclusion:
Circassians from Jordan significantly differ from other populations in terms of the allelic frequencies of
selected VIP variants. The present findings constitute the first set of pharmacogenetic data for Circassian population
from Jordan, providing a basis for safe drug administration that may be useful in diagnosing and treating diseases in
this ethnic group.
Neuro-toxic and Reproductive Effects of BPA
Authors: Santoro Antonietta , Chianese Rosanna , Troisi Jacopo , Richards Sean , Nori Stefania Lucia , Fasano Silvia , Guida Maurizio , Plunk Elizabeth , Viggiano Andrea , Pierantoni Riccardo , Meccariello Rosaria 
Published: July 2019

Background:Bisphenol A (BPA) is one of the highest volume chemicals produced worldwide. It has recognized activity as an endocrine-disrupting chemical and has suspected roles as a neurological and reproductive toxicant. It interferes in steroid signaling, induces oxidative stress, and affects gene expression epigenetically. Gestational, perinatal and neonatal exposures to BPA affect developmental processes, including brain development and gametogenesis, with consequences on brain functions, behavior, and fertility.Methods:This review critically analyzes recent findings on the neuro-toxic and reproductive effects of BPA (and its analogues), with focus on neuronal differentiation, synaptic plasticity, glia and microglia activity, cognitive functions, and the central and local control of reproduction.Results:BPA has potential human health hazard associated with gestational, peri- and neonatal exposure. Beginning with BPA’s disposition, this review summarizes recent findings on the neurotoxicity of BPA and its analogues, on neuronal differentiation, synaptic plasticity, neuroinflammation, neuro-degeneration, and impairment of cognitive abilities. Furthermore, it reports the recent findings on the activity of BPA along the HPG axis, effects on the hypothalamic Gonadotropin Releasing Hormone (GnRH), and the associated effects on reproduction in both sexes and successful pregnancy.Conclusion:BPA and its analogues impair neuronal activity, HPG axis function, reproduction, and fertility. Contrasting results have emerged in animal models and human. Thus, further studies are needed to better define their safety levels. This review offers new insights on these issues with the aim to find the “fil rouge”, if any, that characterize BPA’s mechanism of action with outcomes on neuronal function and reproduction.
The Effects of Smoking Cessation on Diabetes Mellitus Patients
Authors: Alshahrani Ali 
Published: July 2019

Background:
Smoking is an established predictor of type 2 diabetes. However, the link between
smoking cessation and diabetes progression remains a subject of scholarly investigation.

Objective:
The objective of this systematic review is to establish the link between smoking cessation
and diabetes.

Data Sources:
The study utilized conference abstracts and peer-reviewed journals that reported randomized
controlled trials smoking cessation interventions for diabetes patients.

Results:
Results from the review were inconclusive on the link between smoking cessation and diabetes.
On one hand, several researchers have confirmed a positive correlation between smoking cessation
and decreased risk of diabetes. On the other hand, some researchers have demonstrated that immediate
withdrawal of nicotine resulted in increased risk of diabetes; however, this risk reduces with time.

Conclusion:
The result of this review did not estblish a clear relationship between smoking cessation
and diabates.

Limitations:
Compared to other studies examining the implication of smoking on chronic diseases, this
study identified a very small number of trials evaluating the effect of smoking cessation on diabetes.
The small number of studies implies that the results may not be suitable for generalization.

Implication:
Results from the review can help in the development of a tailored intervention for effective
management of diabetes in smoking patients.
Prediction of Risk of Death for Patients Starting Dialysis
Authors: Anderson Ryan T. , Cleek Hailey , Pajouhi Atieh S. , Bellolio M. Fernanda , Mayukha Ananya , Hart Allyson , Hickson LaTonya J. , Feely Molly A. , Wilson Michael E. , Giddings Connolly Ryan M. , Erwin Patricia J. , Majzoub Abdul M. , Tangri Navdeep , Thorsteinsdottir Bjorg 
Published: July 2019

Background and objectives
Dialysis is a preference-sensitive decision where prognosis may play an important role. Although patients desire risk prediction, nephrologists are wary of sharing this information. We reviewed the performance of prognostic indices for patients starting dialysis to facilitate bedside translation.

Design, setting, participants, & measurements
Systematic review and meta-analysis following the PRISMA guidelines. We searched Ovid MEDLINE, Ovid Embase, Ovid Central Register of Controlled Trials, Ovid Cochrane Database of Systematic Reviews, and Scopus for eligible studies of patients starting dialysis published from inception to December 31, 2018. Selection Criteria: Articles describing validated prognostic indices predicting mortality at the start of dialysis. We excluded studies limited to prevalent dialysis patients, AKI and studies excluding mortality in the first 1–3 months. Two reviewers independently screened abstracts, performed full text assessment of inclusion criteria and extracted: study design, setting, population demographics, index performance and risk of bias. Pre-planned random effects meta-analysis was performed stratified by index and predictive window to reduce heterogeneity.

Results
Of 12,132 articles screened and 214 reviewed in full text, 36 studies were included describing 32 prognostic indices. Predictive windows ranged from 3 months to 10 years, cohort sizes from 46 to 52,796. Meta-analysis showed discrimination area under the curve (AUC) of 0.71 (95% confidence interval, 0.69 to 073) with high heterogeneity (I
2=99.12). Meta-analysis by index showed highest AUC for The Obi, Ivory, and Charlson comorbidity index (CCI)=0.74, also CCI was the most commonly used (ten studies). Other commonly used indices were Kahn-Wright index (eight studies, AUC 0.68), Hemmelgarn modification of the CCI (six studies, AUC 0.66) and REIN index (five studies, AUC 0.69). Of the indices, ten have been validated externally, 16 internally and nine were pre-existing validated indices. Limitations include heterogeneity and exclusion of large cohort studies in prevalent patients.

Conclusions
Several well validated indices with good discrimination are available for predicting survival at dialysis start.
Inteligencia emocional y rendimiento académico en estudiantes universitarios de nutrición
Authors: Arntz Vera Jenny , Trunce Morales Silvana 
Published: July 2019

Resumen: El concepto de inteligencia emocional (IE) desarrollado en 1990 por Salovey y Mayer, y Goleman en 1995, nos invita a considerar una nueva visión del estudio de la inteligencia humana más allá de los aspectos cognitivos e intelectuales. El aprendizaje socioemocional en educación y en la formación de profesionales de salud tiene una gran importancia debido a la relación entre competencias emocionales con las competencias genéricas, y a su supuesta asociación con el rendimiento académico. Se realizó una investigación de corte transversal mediante la aplicación del test TMMS-24 a una muestra de 131 estudiantes de la Carrera de Nutrición de una universidad pública chilena, con el objetivo de establecer la relación existente entre los componentes de la inteligencia emocional con el rendimiento académico y avance curricular. Los datos se analizaron mediante estadística descriptiva y se aplicaron pruebas estadísticas de ANOVA para asociación entre niveles de inteligencia emocional y rendimiento académico y prueba de Chi cuadrado para asociación con avance curricular. Los resultados indican que no existen diferencias significativas entre el nivel de atención y el promedio de notas (p = 0.829), el nivel de comprensión y el promedio de notas (p = 0.963) y el nivel de regulación y el promedio de notas (p = 0.501), aplicando ANOVA. No se encontró asociación entre los niveles de inteligencia emocional y avance curricular, aplicando la prueba de chi cuadrado. Los resultados de investigaciones que asocian inteligencia emocional son controversiales. Este estudio es un aporte a los resultados publicados en esta línea. A pesar de los resultados, se considera relevante la consideración de competencias socioemocionales en la formación de profesionales de salud como parte de la formación integral para enfrentar el mundo laboral.