bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitrogen leaching losses from pasture and winter forage crops in the West Matukituki Valley
Authors: Smith Chris , Monoghan Ross , Arbuckle Chris 
Published: October 2024

In 2013 the Otago Regional Council (ORC) entered mediation with local farmers on changes to their Water Plan, Plan Change 6A, which sought to apply nitrogen load limits within the catchments of Lake Wanaka and Lake Hawea for all farm systems. There was little information available on nitrogen leaching losses in the high rainfall environments (c 2500 mm pa) of the South Island's high country. Agreement after mediation with the ORC and local farmers required a research project to examine differences between measured losses and outputs from OVERSEER® at a paddock and farm scale. To address this knowledge gap, a series of monitoring sites were set up in November 2014 in the West Matukituki Valley, west of Lake Wanaka. Porous soil solution samplers were installed to a depth of 50 cm in (i) a sheep- and cattle-grazed pasture, (ii) a cattle-grazed winter forage crop, and (iii) a native bush site. Results for 2015 showed that rainfall totalled 2240 mm, with calculated drainage estimated at 1330 mm. The total fluxes of dissolved N from the crop and pasture sites during the 12 months from June 2015 were 157 and 43 kg N/ha, respectively. Dissolved organic N (DON) made an unexpectedly large contribution to these fluxes, accounting for 20 and 67% of the total fluxes of dissolved N in drainage from the crop and pasture sites, respectively. Gaining a better understanding of the bioavailability of dissolved forms of organic N in leachate would help determine the risks that these forms of nutrients pose to water quality, and if they should be incorporated into models such as Overseer which do not account for DON. The project’s initial findings enabled an informed discussion on the use of modelled results from Overseer for the mitigation of N loss at a farm scale in the lakes’ catchments. It also focussed farmers’ attention on management of the nitrogen leaky parts of their farm systems, especially winter crops.
Epigenetic insights into creep-feeding: methylation profiling of Longissimus thoracis muscle at weaning in crossbred cattle
Authors: Rodrigues Lucas Farias , Ramírez-Zamudio German Dario , Pereira Guilherme Luis , Torrecilhas Juliana Akamine , Trevisan Lucas Augustinho , Machado Neto Otávio Rodrigues , Chardulo Luis Artur Loyola , Baldassini Welder Angelo , Curi Rogério Abdallah 
Published: October 2024

IntroductionThis study investigated the impact of creep-feeding supplementation on the genome methylation of the Longissimus thoracis (LT) muscle in crossbred beef cattle (Bos taurus × Bos indicus).MethodsThe experiment involved 48 uncastrated F1 Angus-Nellore males (half-siblings), which were divided into two groups: NCF – no creep-feeding (n = 24) and CF – creep-feeding (n = 24). After weaning at 210 days, all animals were feedlot finished for 180 days under identical conditions. LT muscle biopsies were collected at weaning for genomic DNA methylation analysis by reduced representation bisulfite sequencing (RRBS).Results and discussionThe groups differed significantly (CF &gt; NCF: p &lt; 0.05) to weaning weight (243.57±5.70 vs. 228.92±5.07kg), backfat thickness (12.96±0.86 vs. 10.61±0.42mm), LT muscle marbling score (366.11±12.39 vs. 321.50±13.65), and LT intramuscular fat content (5.80±0.23 vs. 4.95±0.20%). The weights at the beginning of the experiment and at slaughter (390 days) did not differ significantly. Mean methylation levels were higher in CF with 0.18% more CpG, 0.04% CHG, and 0.03% CHH. We identified 974 regions with differential methylation (DMRs: &gt; 25% and q &lt; 0.05), which overlapped with 241 differentially methylated genes (DMGs). Among these genes, 108 were hypermethylated and 133 were hypomethylated in CF group. Notably, 39 of these DMGs were previously identified as differentially expressed genes (DEGs: log2 fold change [0.5]) in the same animal groups. Over-representation analysis highlighted epigenetic regulations related to muscle growth, PPAR signaling, adipogenesis, insulin response, and lipid metabolism. Key DMGs/DEGs included: ACAA1, SORBS1, SMAD3, TRIM63, PRKCA, DNMT3A, RUNX1, NRG3, and SLC2A8. These epigenetic changes improved the performance of supplemented animals up to weaning and enhanced meat quality traits, particularly higher intramuscular fat. The results provided insights into the intricate interplay between nutrition, epigenetics, gene expression and phenotypes in beef cattle production.
Association between systemic immune-inflammatory index and systemic inflammatory response index with body mass index in children and adolescents: a population-based study based on the National Health and Nutrition Examination Survey 2017-2020
Authors: Luo Lisha , Chen Lin , Song Jukun , Ma Xiuqi , Wang Xike 
Published: October 2024

BackgroundThe Systemic Immune-Inflammatory Index (SII) and Systemic Inflammatory Response Index (SIRI) are novel composite inflammatory markers. Previous studies suggest that obesity in individuals correlates with persistently low levels of chronic inflammation. This study aims to explore the association between SII and SIRI and Body Mass Index (BMI) among children and adolescents.MethodsA cross-sectional survey was conducted using the National Health and Nutrition Examination Survey (NHANES) dataset from 2 consecutive cycles from 2017-2020. Multivariate linear regression models were employed to examine the linear relationships between BMI and SII and SIRI. Non-linear associations were explored using smooth curve fitting and threshold effect analysis.ResultsA total of 2980 children and adolescents aged 6-19 years were included in this population-based study. In the population description of body mass index categories, we found progressively higher levels of SII and SIRI, notably peaking among obese children (SII mean ± SD: 528.83 ± 285.46; SIRI mean ± SD: 1.12 ± 0.79). Weighted multivariate linear regression confirmed a significant positive association between BMI and both inflammatory indices (P &lt; 0.0001). Subgroup analyses revealed consistent correlations across gender divisions and highlighted a non-linear relationship between BMI and SII.ConclusionsSII and SIRI are positively associated with BMI in children and adolescents, indicating their potential as markers for assessing systemic inflammation in pediatric obesity. Further large-scale prospective studies are required to substantiate these findings.
Andrographis paniculata improves glucose regulation by enhancing insulin sensitivity and upregulating GLUT 4 expression in Wistar rats
Authors: Saka W. A. , Oyekunle O. S. , Akhigbe T. M. , Oladipo O. O. , Ajayi M. B. , Adekola A. T. , Omole A. I. , Akhigbe R. E. 
Published: October 2024

ContextAlthough the hypoglycaemic effect of Andrographis paniculata (Burm.f.) Nees [Acanthaceae] has been documented, reports on its effect in an apparently healthy state are limited.ObjectiveThis study investigated whether or not A. paniculata exerts hypoglycaemic effect in a non-diabetic state. It also explored the impact of A. paniculata on glycolytic enzymes and GLUT 4 protein expression, as a possible mode of action.MethodsTwenty male Wistar rats were randomly assigned into two groups (n = 10 rats/group). The control rats were vehicle-treated (0.5 ml of distilled water), while the A. paniculata-treated rats had 500 mg/kg of A. paniculata per os once daily for 35 days.ResultsA. paniculata treatment led to improved insulin sensitivity evidenced by increased HOMA-β (88.08 ± 2.13 vs. 120.80 ± 1.52, p &lt; 0.0001), HOMA-S (283.60 ± 8.82 vs. 300.50 ± 9.30, p = 0.0189), and reduced TyG index (4.22 ± 0.04 vs. 3.95 ± 0.07, p &lt; 0.0002) and HOMA-IR (0.32 ± 0.01 vs. 0.25 ± 0.01, p &lt; 0.0001) when compared with the control. It also improved glucose regulation as depicted by reduced fasting blood glucose (3.77 ± 0.10 vs. 3.24 ± 0.11, p &lt; 0.0001) and glycated hemoglobin (HbA1c; 7.69 ± 1.15 vs. 5.95 ± 0.82, p = 0.0245), and atherogenic dyslipidaemia, including AIP (−0.12 ± 0.03 vs. −0.26 ± 0.03, p &lt; 0.0001) and CRI-I (2.70 ± 0.29 vs. 1.84 ± 0.27, p &lt; 0.0001). These findings were accompanied by enhanced hepatic and muscular redox state, increased activities of glycolytic enzymes, upregulated GLUT 4 (0.80 ± 0.27 vs. 6.20 ± 0.84, p &lt; 0.0001), and increased circulating nitric oxide (5.45 ± 0.24 vs. 6.79 ± 0.33, p = 0.0002).ConclusionA. paniculata exerts positive effect on glucose metabolism and utilization by improving insulin sensitivity and upregulating the activities of glycolytic enzymes and GLUT 4 protein expression. This implies that A. paniculata may be beneficial in preventing insulin resistance and incident diabetes. Nonetheless, it should be used with caution to prevent hypoglycaemia in a non-diabetic state.
Synergistic effect in the co-extraction of Ginseng and Schisandra protein
Authors: Zhang Han , Wang Haidong , Zhou Hongyan , Shi Jialin , Wan Zhiqiang , Li Guangzhe , Yan Mingming 
Published: October 2024

IntroductionGinseng and Schisandra are traditional Chinese plants that have been used in culinary practices and are renowned for their immune-boosting properties. In Chinese medicine, Ginseng and Schisandra are frequently used together as a clinical pair to mutually enhance their effect, producing a synergistic effect when consumed in combination. However, the underlying mechanism of their synergistic effect remains uncertain. Therefore, this study investigates the synergistic effect of Ginseng-Schisandra in terms of macromolecular proteins.MethodsWe used a dual-protein research methodology combined with co-extraction techniques to obtain the co-extracted protein of ginseng and Schisandra. We then compared the physicochemical and functional properties and antioxidant activities of co-extracted protein (COP), simple mixed protein (SMP), Ginseng protein (PGP), and Schisandra protein (SCP).ResultsGenerally, PGP and SCP are considered as functional food with antioxidant activity. COP are composite proteins with a shared internal structure that are combined by Ginseng and Schisandra proteins, while SMP are simple mixtures of PGP and SCP. Free radical scavenging experiments indicated that COP exhibited the highest scavenging ability for hydroxyl radicals (98.89%), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals (85.95%), and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS+) radicals (42.69%). In vitro, COP significantly reduced the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), while increasing intracellular levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and lactate dehydrogenase (LDH) levels in HepG2 cells.DiscussionThe comparative results of the macromolecular proteins reveal that COP contributes to the synergistic effect of Ginseng-Schisandra and indicate the advantages of co-extraction in protein production, suggesting the potential application of COP in the food industry.
A computational assay for identifying millet-derived compounds that antagonize the interaction between bisphenols and estrogen-related receptor gamma
Authors: Pathak Rajesh Kumar , Kim Jun-Mo 
Published: October 2024

The use of Bisphenol A (BPA) and its analogs in industries, as well as the products made from them, is becoming a significant concern for human health. Scientific studies have revealed that BPA functions as an endocrine disruptor. While some analogs of BPA (bisphenols) have been used for a longer time, it was later discovered that they are toxic, similar to BPA. Their widespread use ensures their presence in the environment, and thus, everyone is exposed to them. Scientific research has shown that BPA interacts with estrogen-related receptor gamma (ERRγ), affecting its normal function. ERRγ is involved in biological processes including energy metabolism and mitochondrial function. Therefore, continuous exposure to bisphenols increases the risk of various diseases. In our previous study, we observed that some analogs of BPA had a higher binding affinity to ERRγ compared to BPA itself and analyzed the amino acid residues involved in this interaction. We hypothesized that by antagonizing the interaction between bisphenols and ERRγ, we could neutralize their toxic effects. Taking into account the health benefits of millets and their toxin removal properties, virtual screening of millet-derived compounds was conducted along with prediction of their ADMET profiles. Top five candidates were prioritized for Density Functional Theory (DFT) calculations and further analyses. Long-term molecular dynamics simulation (1 µs) were utilized to evaluate their binding, stability, and antagonizing abilities. Furthermore, reevaluation of their binding energy was conducted using the MM-PBSA method. This study reports millet-derived compounds, namely, Tricin 7-rutinoside, Tricin 7-glucoside, Glucotricin, Kaempferol, and Setarin. These compounds are predicted to be potent competitive inhibitors that can antagonize the interactions between bisphenols and ERRγ. These compounds could potentially assist in the development of future therapeutics. They may also be considered for use as food supplements, although further investigations, including wet-lab experiments and clinical studies, are needed.
(Pro)renin receptor aggravates myocardial pyroptosis in diabetic cardiomyopathy through AMPK-NLRP3 pathway
Authors: Li Shengnan , Zhang Jingjing , Zhao Yuewen , Kang Li , Jie Haipeng , Dong Bo 
Published: October 2024

IntroductionAs one of the most common complications of diabetes, diabetic cardiomyopathy (DCM) is the main cause of heart failure in patients with diabetes. However, the lack of effective treatments for DCM remains a clinical challenge. (Pro) renin receptor (PRR) is a member of renin angiotensin aldosterone system (RAAS). Here, we aim to determine whether PRR is involved in myocardial pyroptosis in diabetic cardiomyopathy.MethodsWe established diabetic rats model by intraperitoneal injection of streptozotocin (STZ). PRR overexpression adenovirus or PRR knockdown adenovirus was injected into the tail vein. Western blot, histopathology and immunohistochemistry staining, ELISA and Echocardiography were used to detect cardiac function changes and myocardial injury levels of DCM rats. Primary cardiomyocytes were stimulated with high glucose and PRR overexpression or PRR knockdown was achieved by adenovirus transfection, we also used the inhibitor of AMPK to decrease the activity of AMPK. Western blot, Real-time PCR, Immunofluorescence and ELISA were used to detect the level of PRR and pyroptosis in cardiomyocyte.ResultsWe found that high glucose increased the expression of PRR in heart. After overexpression of PRR, the expression of the pyroptosis related proteins such as Caspase-1, IL-1β, IL-18, and NLRP3 was significantly increased, the phosphorylation level of AMPK was significantly decreased, and the fibrosis level was significantly increased, thus aggravating the cardiac function injury of DCM. On the contrary, PRR knockdown can alleviate the level of myocardial pyroptosis in DCM and improve cardiac function. The related mechanism was that PRR could inhibit AMPK phosphorylation and promote the activation of NLRP3 inflammasome.DiscussionPRR aggravated pyroptosis of cardiomyocyte, increased the dysfunction of cardiomyocyte, and may be related to the decrease of AMPK phosphorylation and the overactivation of NLRP3. This may provide new ideas and targets for the treatment of DCM.
Preparation and Characterization of Materials for Low- to Intermediate-Temperature CO2 Adsorption
Authors: Figueiredo Anabela , Soria Miguel Angel , Madeira Luís Miguel , Rocha Cláudio 
Published: October 2024

Global carbon dioxide emissions are rising and the use of fossil fuels in several sectors are the leading causes. As global population and economies continue to grow significantly, the most practical method of lowering such emissions is to capture CO2. Although other technologies are more developed, adsorption is very promising and has attracted much attention. To ensure this technology’s success, it is essential to have suitable CO2 adsorbent materials. In this work, several new hydrotalcites (HTs) with different initial concentrations of ion precursors were prepared for the first time by the co-precipitation method—it was possible to verify that the ion concentrations influence the characteristics of the materials. The prepared HTs were characterized by thermogravimetric analysis (TG), X-Ray diffraction (XRD), surface area measurements and temperature-programmed desorption of CO2 (TPD-CO2) to relate their CO2 capture capacity to their physicochemical properties; the CO2 adsorption equilibrium isotherms were determined at 35 and 300 °C for the prepared samples, as well as for some commercial materials: magnesium oxide, calcium oxide, aluminium oxide and Zeolite 13X. After determining which materials present the best CO2 adsorption capacity, these were submitted to adsorption-desorption cycles to study their stability. The main objective of the work was to prepare and study different CO2 adsorbents for processes that are carried out at low and intermediate temperatures. From the experimental results, it was possible to conclude that the Zeolite 13X showed the best capacity at 35 °C, 3.38 mmol·g−1 (@ pCO2 = 1 bar), and a prepared calcined HT (c-HT2) was the best at 300 °C, 0.97 mmol·g−1 (@ pCO2 = 1 bar). Moreover, it seems there is an optimum initial concentration of the ions’ solutions for the tested HTs, which depends on the final application—c-HT1 showed a better capacity at 35 °C and c-HT2 at 300 °C. From the adsorption-desorption cycles—performed at 35 and 300 °C with the best materials using a magnetic suspension microbalance at 1 bar of CO2 partial pressure —, a working cyclic capacity of 2.69 mmol∙g−1 was achieved by the Zeolite at 35 °C; in turn, c-HT2 showed a working cyclic capacity of 0.79 mmol∙g−1 at 300 °C.
Klasifikasi Penyakit Monkeypox dengan Menggunakan Algoritma K-Nearest Neighbor
Authors: Suyanti  , Arvita Yulia , Siswanto Agus 
Published: October 2024

Dengan berkembangnya penyakit mokeypox maka banyak masyarakat bisa tertular penyakit ini, di karenakan peyakit ini penularannya bisa melalui kontak erat dengan hewan/orang yang terinfeksi monkeypox. Penyebaranya bisa melalui kontak tatap muka, kulit ke kulit, mulut ke mulut, atau mulut ke kulit, termaksud kontak seksual, percikan ludah/cairan hidung, dan mungkin penularan bisa melalui aerosol jarak pendek. jika virus ini tertular ke bayi yang baru lahir, anak – anak, dan orang dengan gangguan kekebalan tubuh maka dapat beresiko mengalami gejala – gejala yang lebih serius dan menyebabkan kematian. Untuk mengurangin penyebaran penyakit ini dapata dilakukan diagnosa terhadap faktor – faktor terkait, akan tetapi terdapat resiko jika salah dalam mengklasifikasikan. Untuk mengatasi hal tersebut maka dapat memanfaatkan metode dalam data mining. Data mining merupakan proses yang menemukan pengenalan pola dan pengklasifikasian data besar dengan tujuan mendapatkan hubungan atau pola yang dapat memberikan manfaat. Untuk menghentikan penyebaran virus monkey pox secara luas maka dengan melakukan klasifikasi dengan menggunakan metode KNN penyakit monkeypox bisa di klasifikasikan menurut gejalanya. Sehingga hal ini bisa menghentikan penyebaraan virus semakin banyak dan meluas serta bisa mendeteksi dini penyakit monkeypox dan menghambat penularan serta pertumbuhan kematian yang di akibatkan oleh virus ini
Fuzzy Time Series Model Lee Dalam Memprediksi Nilai Ekspor di Indonesia
Authors: Yulanda Rahmadiyah  , Syamsyida Rozi  , Corry Sormin  , Yuliana Safitri  
Published: October 2024

Ekspor adalah indikator penting dalam perekonomian Indonesia. Menurut Badan Pusat Statistik (BPS), nilai ekspor Indonesia pada bulan April 2022, mencapai US$ 27.316,2 juta, meningkat 47,76% dibandingkan bulan sebelumnya. Berdasarkan hal itu terlihat bahwa nilai ekspor di Indonesia cenderung tidak stabil. Hal tersebut dipengaruhi oleh fluktuasi nilai tukar, perubahan permintaan pasar, dan faktor eksternal lainnya, sehingga menyulitkan perencanaan bisnis dan perdagangan antar negara. Oleh karena itu, diperlukan strategi pengendalian nilai ekspor dengan melakukan prediksi di masa mendatang. Penelitian ini bertujuan memprediksi nilai ekspor di Indonesia menggunakan metode Fuzzy Time Series Lee dan mengukur akurasinya dengan Mean Absolute Percentage Error (MAPE). Data yang digunakan adalah data sekunder yang diperoleh dari Badan Pusat Statistik yaitu data nilai ekspor dari Januari 2021 hingga Februari 2024. Langkah-langkah dalam metode ini meliputi menentukan himpunan semesta, pembentukan interval, menentukan himpunan fuzzy, fuzzifikasi data, penentuan Fuzzy Logic Relationship (FLR), pembentukan Fuzzy Logic Relationship Group (FLRG), defuzzifikasi, dan hasil prediksi. Nilai akurasi metode prediksi dihitung menggunakan Mean Absolute Percentage Error (MAPE). Hasil penelitian menunjukkan bahwa nilai akurasi prediksi dengan Fuzzy Time Series Lee memiliki tingkat kesalahan 5,1568% dan tingkat akurasinya sebesar 94,843%. Nilai MAPE tersebut termasuk kategori sangat baik karena kurang dari 10%. Prediksi nilai ekspor Indonesia menggunakan metode Fuzzy Time Series Lee pada bulan Maret 2024 adalah US$ 21.850,69 juta.
Ultrasound Pancreatic Stimulation: A Novel Strategy for Insulin Release and Diabetes Management
Authors: Khalafpour Niloufar 
Published: October 2024

Diabetes mellitus remains a global health care challenge, promoting the search for innovative treatments. In vivo and in vitro studies have shown the potential benefits of ultrasound (US) on enhancing insulin release and reactivating pancreatic β cell function. As a novel, nonpharmacological, noninvasive, and cost-effective approach, US therapy holds promise for stimulating pancreatic function and improving insulin secretion. This review explores recent findings on US therapy, focusing on low-intensity US (LIUS) and its effects on varying treatment parameters. Despite promising results, conflicting evidence highlights the need for further investigation through large-scale clinical trials to establish the therapeutic potential of US therapy and to optimize treatment regimens for effective diabetes management. The biological response to LIUS is complex and involves multiple cell types and pathways. The mechanisms triggering these effects require further exploration. A future engineering challenge lies in designing an experimental setup to control the US-induced mechanical phenomena, enabling the evaluation of biological effects with respect to parameters such as intensity, frequency, or duty cycle.