bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Electrospun Carbon−Carbon Composite and Commercial Felt for Their Activity and Electrolyte Utilization in Vanadium Redox Flow Batteries
Authors: Fetyan Abdulmonem , Schneider Jonathan , Schnucklake Maike , El‐Nagar Gumaa A. , Banerjee Rupak , Bevilacqua Nico , Zeis Roswitha , Roth Christina 
Published: October 2018

AbstractA low cost highly active carbon−carbon composite fiber felt was produced by electrospinning a mixture of polyacrylonitrile and carbon black powder using poly acrylic acid as a binder for high carbon black loading. The newly designed high‐surface area electrode material showed promising results for use as electrode material for both the negative and positive half‐cell of vanadium redox flow batteries. Battery test results demonstrated promising performance for the electrospun carbon fibers at current densities below 60 mA cm−2, but were less active at higher values. The microstructure of the felt was characterized by X‐ray computed tomography to obtain the porous pathways, which facilitate electrolyte transport. The obtained results will help us to understand the role of porosity in the performance of the battery and to consequently improve the design of the carbon‐filled electrospun material.
One‐Pot Synthesis of a Highly Active 3‐Dimensional Fe−Nx−CNTs/rGO Composite Catalyst for Oxygen Reduction
Authors: Hu Ye , Yang Ruoou , Chen Hengquan , Han Shaobo , Wang Junhu , Wang Xiaojiang , Deng Xin , He Chao‐Hong , He Qinggang , Jiang Zheng , Gu Meng 
Published: October 2018

AbstractA facile one‐pot method to synthesize a Fe−Nx−CNTs/rGO catalyst by using melamine and FeCl2⋅4H2O as low‐cost precursors is reported.The 3‐dimensional porous structure and the dispersion of precursor were completed at the same time. Suitable precursor and concentration selection not only solved the aggregation of graphene but also improved its effective iron content and activity. The obtained catalysts exhibited oxygen reduction activitycomparable to commercial Pt/C with a half‐wave potential (the potential when current density reaches the half of the diffusion‐limited current density) at 0.875 V(vs. RHE) in alkaline media. In addition, it also showed a better stability than Pt/C with a lower decay of E1/2 (10 mV) after 2000 cycles. From the systematic physical characterizations including aberration‐corrected scanning transmission electron microscopy, 57Fe Mössbauer spectroscopy and X‐ray absorption spectroscopy, it was discovered that a unique structure with the coexistence of nitrogen‐coordinated single iron atoms and Fe/Fe2C nanocrystals had been formed.
Entrepreneurial orientation and performance in private family firms: A configurational model
Authors: Bauweraerts Jonathan 
Published: October 2018

AbstractThe relationship between entrepreneurial orientation (EO) and performance has received considerable attention from the family business literature. However, little is known about how EO, organizational parameters, and environmental characteristics act in combination to influence family firm performance. Drawing on the socioemotional wealth perspective, this study proposes a configurational model that considers the performance implications of EO, family management, and environmental dynamism. The empirical analysis indicates that the combination of family management, low EO, and low environmental dynamism engenders the best performance. The paper contributes to the family entrepreneurship literature by identifying the conditions under which EO leads to improved performance in family firms, and answers a recent call for more context‐based investigations of the performance effects of family managers. © 2018 ASAC. Published by John Wiley & Sons, Ltd.
From Munsell color system to a new color psychology system
Authors: Horiguchi Setsuko , Iwamatsu Katsura 
Published: October 2018

AbstractShigenobu Kobayashi, the founder of Nippon Color and Design Research Institute Inc., developed a Hue and Tone System based on the Munsell Color System, referring to the ISCC‐NBS method of designating color and a dictionary of color names during the process. A particularly noteworthy feature of this system is its tone settings, which successfully represent the entire world of color in just 130 colors. Kobayashi used these 130 systematically and psychologically representative colors as research samples in various studies using methods such as the semantic differential technique, and developed a Color Image Scale that clearly demonstrated the semantic space of colors. The present article will describe the development and impact of the Hue and Tone System and Color Image Scale since Kobayashi's article The Aim and Method of the Color Image Scale was published in Color Research and Application in 1981.
Refolding Proteins from Inclusion Bodies using Differential Scanning Fluorimetry Guided (DGR) Protein Refolding and MeltTraceur Web
Authors: Lee Mark E. , Dou Xiaoyi , Zhu Yingmin , Phillips Kevin J. 
Published: October 2018

AbstractDifferential Scanning Fluorimetry Guided Refolding (DGR) is a simple methodology that can be used to rapidly screen for and identify conditions capable of accurately refolding protein preparations, such as those obtained from Escherichia coli inclusion bodies. It allows for the production in E. coli of functional proteins that would otherwise require far more expensive production methods. This unit describes how to set up a DGR refolding assay, perform DGR refolding trials in microplate format, use MeltTraceur Web software to interactively analyze the resulting data, scale‐up protein production via refolding, and lastly, validate that the protein is properly folded. © 2018 by John Wiley & Sons, Inc.
Polysome Profiling Analysis of mRNA and Associated Proteins Engaged in Translation
Authors: Pringle Eric S. , McCormick Craig , Cheng Zhenyu 
Published: October 2018

AbstractPost‐transcriptional regulation is an important aspect of the control of gene expression. mRNAs are translated with variable efficiencies, and these efficiencies can change rapidly during adaptation to diverse environmental factors, including cellular stresses and microbial infections. Polysome profiling analysis utilizes ultracentrifugation to isolate complexes of mRNAs in the process of translation and corresponding proteins on the basis of density. Here we describe polysome profiling analysis using a continuous ultraviolet spectrophotometer and a gradient fractionator. We provide protocols for processing sucrose gradient fractions for isolation of RNA for RT‐qPCR analysis and isolation of protein for SDS‐PAGE and immunoblot analysis. © 2018 by John Wiley & Sons, Inc.
Retrospective assessment of the effectiveness of the Milan system for reporting salivary gland cytology: A systematic review and meta‐analysis of published literature
Authors: Farahani Sahar J , Baloch Zubair 
Published: October 2018

AbstractIntroductionFine needle aspiration (FNA) has been widely utilized in establishing the nature of salivary gland lesions and guiding the clinical management. This study aimed to determine the accuracy of FNA in detecting salivary gland neoplasms and malignancies, employing the “Milan System for Reporting Salivary Gland Cytopathology” (MSRSGC).MethodA systematic search was conducted. The data on FNA and histologic diagnosis were extracted and categorized based on the MSRSGC and risk of malignancy (ROM) was calculated. The risk of publication bias and level of heterogeneity were evaluated. A mixed‐effects model was used to estimate FNA accuracy. Meta‐regression was conducted to assess the potential effect of different variables on FNA accuracy.ResultsNinety‐two studies with a total of 16 456 FNA with surgical follow‐up were included. ROM was estimated as 17%, 8%, 34%, 4%, 42%, 58%, and 91%, in nondiagnostic, nonneoplastic, atypia of undetermined significance, benign neoplasm, salivary gland neoplasm of uncertain malignant potential, suspicious for malignancy, and malignant groups, respectively. High level of heterogeneity was detected (P‐value <.001). Including cases with definite FNA diagnosis of neoplasm or malignancy, summary estimates of FNA sensitivity, specificity, diagnostic odds ratio, and positive and negative likelihood ratio in detecting neoplasms and malignancies were 96.9%, 95.3%, 636.8, 20.5, and 0.03, and 80.5%, 97.9%, 189.5, 37.8, and 0.2, respectively. Meta‐regression showed several variables significantly impacting FNA accuracy; however, subgroup analysis did not reduce the level of heterogeneity.ConclusionFNA can be used as a reliable diagnostic tool in the preoperative evaluation and management of salivary glands lesions. Concise of abstract is using Milan system for reporting salivary gland FNA could increase FNA reliability, facilitate communication, and improve patient care.
Inhibitory effect of isavuconazole, ketoconazole, and voriconazole on the pharmacokinetics of methadone in vivo and in vitro
Authors: Shen Leibin , Wang Jun , Yi Yongdong , Ye Chenmin , Wang Rongrong , Xia Guojun , Yu Chengyang , Tu Fuyang , Xu Jingxuan , Zheng Zhiqiang 
Published: October 2018

AbstractThe aim of this study was to investigate the possible effect of orally administered isavuconazole, ketoconazole, or voriconazole on the pharmacokinetics of methadone in rats. Twenty Sprague–Dawley (SD) rats were divided randomly into four groups: Group A (control), group B (5 mg/kg isavuconazole), group C (5 mg/kg ketoconazole), and group D (5 mg/kg voriconazole). A single dose of methadone was administrated half an hour later. Methadone in plasma concentrations and its metabolite EDDP in microsomes were determined by ultra‐high‐performance liquid chromatography–tandem mass spectrometry method (UPLC–MS/MS), and pharmacokinetic parameters were calculated by DAS version 3.0. The Cmax of methadone in groups C and D increased to 2.7‐fold and 5‐fold, respectively. While AUC increased in three groups and group D increased the most. Also, isavuconazole, ketoconazole, and voriconazole showed inhibitory effect on human and rat microsomes. The inhibition ratios of isavuconazole, ketoconazole, and voriconazole in rat liver microsome were 97.87%, 96.74% and 78.9%, respectively (p < 0.01), while in human liver microsome, inhibition ratios were 86.97%, 96.46%, and 53.11%, respectively. And the IC50 for inhibition activity of isavuconazole, ketoconazole, and voriconazole in rat microsomes were 7.76 μM, 8.33 μM, and 4.45 μM, respectively. Our study indicated that taking methadone combine with ketoconazole, isavuconazole, or voriconazole could reduce the metabolism rate of methadone and prolong the pharmacological effects in vivo and in vitro.
Hidden cost of disease in a free‐ranging ungulate: brucellosis reduces mid‐winter pregnancy in elk
Authors: Cotterill Gavin G. , Cross Paul C. , Middleton Arthur D. , Rogerson Jared D. , Scurlock Brandon M. , du Toit Johan T. 
Published: October 2018

AbstractDemonstrating disease impacts on the vital rates of free‐ranging mammalian hosts typically requires intensive, long‐term study. Evidence for chronic pathogens affecting reproduction but not survival is rare, but has the potential for wide‐ranging effects. Accurately quantifying disease‐associated reductions in fecundity is important for advancing theory, generating accurate predictive models, and achieving effective management. We investigated the impacts of brucellosis (Brucella abortus) on elk (Cervus canadensis) productivity using serological data from over 6,000 captures since 1990 in the Greater Yellowstone Ecosystem, USA. Over 1,000 of these records included known age and pregnancy status. Using Bayesian multilevel models, we estimated the age‐specific pregnancy probabilities of exposed and naïve elk. We then used repeat‐capture data to investigate the full effects of the disease on life history. Brucellosis exposure reduced pregnancy rates of elk captured in mid‐ and late‐winter. In an average year, we found 60% of exposed 2‐year‐old elk were pregnant compared to 91% of their naïve counterparts (a 31 percentage point reduction, 89% HPDI = 20%–42%), whereas exposed 3‐ to 9‐year‐olds were 7 percentage points less likely to be pregnant than naïve elk of their same age (89% HPDI = 2%–11%). We found these reduced rates of pregnancy to be independent from disease‐induced abortions, which afflict a portion of exposed elk. We estimate that the combination of reduced pregnancy by mid‐winter and the abortions following mid‐winter reduces the reproductive output of exposed female elk by 24%, which affects population dynamics to a similar extent as severe winters or droughts. Exposing hidden reproductive costs of disease is essential to avoid conflating them with the effects of climate and predation. Such reproductive costs cause complex population dynamics, and the magnitude of the effect we found should drive a strong selection gradient if there is heritable resistance.
Local adaptation of photoperiodic plasticity maintains life cycle variation within latitudes in a butterfly
Authors: Lindestad Olle , Wheat Christopher W. , Nylin Sören , Gotthard Karl 
Published: October 2018

AbstractThe seasonal cycle varies geographically and organisms are under selection to express life cycles that optimally exploit their spatiotemporal habitats. In insects, this often means producing an annual number of generations (voltinism) appropriate to the local season length. Variation in voltinism may arise from variation in environmental factors (e.g., temperature or photoperiod) acting on a single reaction norm shared across populations, but it may also result from local adaptation of reaction norms. However, such local adaptation is poorly explored at short geographic distances, especially within latitudes. Using a combination of common‐garden rearing and life cycle modeling, we have investigated the causal factors behind voltinism variation in Swedish populations of the butterflyPararge aegeria, focusing on a set of populations that lie within a single degree of latitude but nonetheless differ in season length and voltinism. Despite considerable differences in ambient temperature between populations, modeling suggested that the key determinant of local voltinism was in fact interpopulation differences in photoperiodic response. These include differences in the induction thresholds for winter diapause, as well as differences in photoperiodic regulation of larval development, a widespread but poorly studied phenomenon. Our results demonstrate previously neglected ways that photoperiodism may mediate insect phenological responses to temperature, and emphasize the importance of local adaptation in shaping phenological patterns in general, as well as for predicting the responses of populations to changes in climate.
High Sensitive Detection of Prostate Specific Antigen by Using Ferrocene Cored Asymmetric PAMAM Dendrimer Interface Screen Printed Electrodes
Authors: Cevik Emre 
Published: October 2018

AbstractIn this study, electrochemical immunosensors were developed for the detection of prostate specific antigen (PSA) using ferrocene (Fc) and polyamidoamine dendrimer (PAMAM) constructs. The biosensor fabrication was designed by modifying the screen‐printed gold electrode (Au) with ferrocene cored dendrimers (FcPAMAM) synthesized in three different generations. The self‐assembled monolayer principle was followed, to obtain sensitive, selective and disposable electrodes. Therefore, the Au electrodes were modified with cysteamine (Cys) to obtain a functional surface for FcPAMAM dendrimers to bind. Dendrimer generations were attached to this surface using a cross‐linker (glutaraldehyde) so that a suitable surface was obtained for binding of biological components. The Monoclonal PSA antibody (anti‐PSA) was immobilized on the Au electrode surface which coated with dendrimer, and (Au/Cys/FcPAMAM/anti‐PSA) biosensing electrode was obtained. The PSA detection performances of electrochemical impedance spectroscopy (EIS) and Amperometry based immunosensors exhibited very low detection limits; 0.001 ng mL−1 and 0.1 pg mL−1, respectively. In addition, EIS and Amperometry based biosensors using Au/Cys/FcPAMAM/anti‐PSA sensing electrode were represented excellent linear ranges of 0.01 ng mL−1 to 100 ng mL−1 and 0.001 ng mL−1 to 100 ng mL−1. In order to determine the applicability recovery and selectivity tests were performed using three different proteins in human serum.
Modeling crop breeding for global food security during climate change
Authors: Cowling Wallace A. , Li Li , Siddique Kadambot H. M. , Banks Robert G. , Kinghorn Brian P. 
Published: October 2018

AbstractSimultaneous genetic improvements in grain yield and heat stress tolerance (HST) are necessary to avoid a fall in crop yields caused by global warming during the 21st century. Future food security depends on crop breeding solutions to this challenge, especially in developing countries where the need is greatest. We stochastically model a wheat breeding program during 60 years of rapid global warming based on rapid 2‐year cycles, with selection in early generations for HST, grain yield, disease resistance, and stem strength. In each cycle, breeding values were estimated by best linear unbiased prediction using all pedigree and phenotypic information (including selfing) back to the founders. We compared two methods of selection and mating design with similar costs. The first method was truncation selection for HST to match predicted increases in land temperatures followed by selection for an economic index composed of weighted estimated breeding values for each trait, followed by random pair‐wise mating among selections. The second method was optimal contributions selection (OCS) for the economic index with an overriding constraint to increase HST in each cycle to match global warming trends, and mating prescribed by OCS. Truncation selection caused a rapid loss of genetic diversity, and HST did not keep pace with global warming. Consequently, grain yield began to decline due to heat stress before 60 years. With OCS, HST matched global warming trends, the economic index almost tripled and grain yield nearly doubled during 60 years of global warming. OCS on an economic index, with a priority to meet HST, increased grain yields and avoided a major threat to global food security caused by global warming.
Statistical study on the environmental effects on the natural variation of nutritional components in rice varieties
Authors: Oh Seon‐Woo , Lee Soyoung , Park Sooyun , Lee Sanggu , Lee Seongkon , Cho Hyunsuk , Chung Youngsoo , Park Soonki 
Published: October 2018

AbstractThis study was investigated to compare the natural variation of nutrients in rice variety by different environmental factors. Fifteen kinds of rices were used, which were cultivated in two locations for 2 years. All data were analyzed by the various statistical tools to identify the nutritional variations of nutrients. The results of variable importance in the prediction analysis were found to be consistent with the % variability. The nutrient compositions most affected by variety were fatty acids, and next were vitamins, proximate nutrients, minerals, and amino acids in order. The nutrient compositions most affected by location were proximate, followed by minerals, vitamins, fatty acids, and amino acids. For cultivation year, vitamins were most affected and then minerals, fatty acids, proximate nutrients, and amino acids in order. These findings could explain that each kind of nutrients can be naturally varied by different environmental factors.
Optimization of cellulase production under solid‐state fermentation by a new mutant strain of Trichoderma reesei
Authors: Darabzadeh Nazanin , Hamidi‐Esfahani Zohreh , Hejazi Parisa 
Published: October 2018

AbstractNowadays, the use of agricultural by‐products, as the cheap substrate for the production of value‐added products, is of high interest for the researchers and practitioners. Cellulase is a relatively expensive and a very important industrial enzyme where in this study was produced form rice by‐products under solid‐state fermentation. A new mutant of Trichoderma reesei was used for cellulase production. The effective variables were initially screened by “Plackett and Burman design.” Afterward, the main variables including moisture content, P source, incubation temperature, and incubation time were optimized by “one factor at a time design.” Finally, the resulting variables including 74% for moisture content, 2 g/L for K2HPO4, 30°C of incubation temperature, and 4 days of incubation time were reported as the ultimate optimal condition for cellulase production.
Robust Remote Sensing of Trace‐Level Heavy‐Metal Contaminants in Water Using Laser Filaments
Authors: Li Helong , Zang Hongwei , Xu Huailiang , Sun Hong‐Bo , Baltuška Andrius , Polynkin Pavel 
Published: October 2018

AbstractWater is the major natural resource that enables life on our planet. Rapid detection of water pollution that occurs due to both human activity and natural cataclysms is imperative for environmental protection. Analytical chemistry–based techniques are generally not suitable for rapid monitoring because they involve collection of water samples and analysis in a laboratory. Laser‐based approaches such as laser‐induced breakdown spectroscopy (LIBS) may offer a powerful alternative, yet conventional LIBS relies on the use of tightly focused laser beams, requiring a stable air–water interface in a controlled environment. Reported here is a proof‐of‐principle, quantitative, simultaneous measurement of several representative heavy‐metal contaminants in water, at ppm‐level concentrations, using ultraintense femtosecond laser pulses propagating in air in the filamentation regime. This approach is straightforwardly extendable to kilometer‐scale standoff distances, under adverse atmospheric conditions and is insensitive to the movements of the water surface due to the topography and water waves.
Heterogeneity of the astrocytic AMPA‐receptor transcriptome
Authors: Mölders Andrea , Koch Angela , Menke Raphael , Klöcker Nikolaj 
Published: October 2018

AbstractAstrocytes form the largest class of glial cells in the central nervous system. They serve plenty of diverse functions that range from supporting the formation and proper operation of synapses to controlling the blood–brain barrier. For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance. AMPARs form as macromolecular protein complexes, whose composition of the pore‐lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior. Although astrocytic AMPARs have been reported to exhibit heterogeneous properties, their molecular composition is largely unknown. In this study, we sought to quantify the astrocytic AMPAR transcriptome during brain development and with respect to selected brain regions. Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions. Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes. Our study provides a workflow and a first reference for future investigations into the molecular and functional diversity of glial AMPARs.