bluuebunny/crossref_metadata_2025_split huggingface dataset:
RAD50 predicts the prognosis of hepatitis B virus-related hepatocellular carcinoma
Authors: Liu Wangrui , Xu Wenhao , Chen Yuyan , Sun Xiaolei , Gu Liugen , Qu Yuanyuan , Zhang Jingxin , Huang Haineng , Zhang Hailiang , Ye Dingwei , Liu Xiaojuan 
Published: July 2019

Abstract
RAD50, which is involved in the DNA double-strand break (DSB) repair process, is also involved in cancer outcomes. However, its role in hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) remains unclear. RAD50 increased in HCC tissues compared to normal tissues and was correlated with OS in the TCGA cohort. The validation analysis indicated that increased RAD50 was expressed in HCC tissues in the two independent cohorts, 107 and 100 patients from the Affiliated Hospital of Youjiang Medical University of Nationalities (AHYMUN) and the Affiliated Hospital of Nantong University (AHNTU), respectively. In addition, HCC patients with elevated RAD50 expression showed poor OS and DFS in the AHYMUN cohort and decreased OS and DFS in the AHNTU cohort. Furthermore, four datasets obtained from the Oncomine database validated the analysis of the differential expression of RAD50 in HCC tumors and normal tissues. In conclusion, our study reveals that elevated RAD50 expression is correlated with cancer progression and poor survival in HBV-related HCC patients. These data suggest that RAD50 may act as an oncogene and may serve as a promising therapeutic target for HCC patients.
Effect of Normal Range Serum Thyrotrophin Levels on Bone Mineral Density of post-menopausal women and older men
Authors: Liu Jingfang , Qin Liping , Fu Songbo , Tang Xulei , Ma Lihua , Wang Binli , Li Yujuan , Wu Dan , Sun Weiming , Niu Ying , JIng Gaojing 
Published: July 2019

Abstract
Objective: To analyze the effect of serum TSH levels within the normal range on bone mineral density (BMD) of post-menopausal women and older men. Methods: A total of 570 subjects (305 older men aged 50 years or older and 265 post-menopausal women) with normal thyroid function were enrolled. According to the tertiles of serum normal TSH levels, men (T1, T2, and T3), and post-menopausal women (N1, N2, and N3) were divided into three groups, respectively. The differences in blood calcium, phosphorus, 25(OH) vit D, right calcaneal and left forearm BMD levels between the groups with different TSH levels were compared. Results: The right calcaneus BMD in T1 or T2 group of men was significantly lower than those in T3 group (all P<0.05). The right calcaneus and left forearm BMD in N1 or N2 group of post-menopausal women was significantly lower than those in N3 group (both P<0.05). There was no significant difference in serum calcium and phosphorus levels, 25 (OH) vit D between the three groups with different TSH levels of men or post-menopausal women (all P>0.05). The prevalence of right calcaneal osteoporosis of N1 or N2 group, and the prevalence of left forearm osteoporosis of N1 group in postmenopausal women was significantly higher than those of N3 group (all P<0.05). The TSH levels were significantly positively correlated with right calcaneal BMD of men (R=0.116, P=0.044), and positively correlated with right calcaneal and left forearm BMD of post-menopausal women (R=0.198 and R=0.135, P<0.05). BMD considered as the dependent variables, after adjusting for gender, age, BMI, waist circumference, serum calcium, phosphorus and 25 (OH) vit D levels, the risks of right calcaneal osteoporosis in N1 group of post-menopausal women were 2.278 times that of N3 group. The different TSH levels in men showed no association with the risks of osteoporosis (all P>0.05).
Conclusions: The normal serum TSH levels of men or post-menopausal women were related to BMD. The TSH levels within the lower normal range were associated with the risks of osteoporosis in post-menopausal women.
Peek Community Eye Health - mHealth system to increase access and efficiency of eye health services in Trans Nzoia County, Kenya: study protocol for a cluster randomised controlled trial.
Authors: Rono Hillary , Bastawrous Andrew , Macleod David , Wanjala Emmanuel , Gichuhi Stephene , Burton Matthew 
Published: July 2019

Abstract
Background: Globally eye care provision is currently insufficient to meet the requirement for eye care services. Lack of access and awareness are key barriers to specialist services, in addition, specialist services are over utilised by people with conditions that could be managed in the community or primary care. In combination, these lead to a large unmet need for eye health provision.

We have developed a validated smart phone-based screening algorithm (Peek Community Screening App). The application (App) is part of the Peek Community Eye Health system (Peek CEH) that enables Community Volunteer (CVs) to make referral decisions about patients with eye problems. It generates referrals, automated short messages service (SMS) notifications to patients or guardians and has a program dashboard for visualizing service delivery.

We hypothesize that a greater proportion of people with eye problems will be identified using the Peek CEH system and that there will be increased uptake of referrals, compared to those identified and referred using the current community screening approaches.

Study design: A single masked, cluster-randomised controlled trial design will be used. The unit of randomisation will be the “community unit”, defined as a dispensary or health centres with its catchment population. The community units will be allocated to receive either the intervention (Peek CEH system) or the current care (periodic health centre-based outreach clinics with onward referral for further treatment). In both arms, a triage clinic will be held at the link health facility four weeks from sensitization, where attendance will be ascertained. During triage participants will be assessed and treated, and if necessary referred onwards to Kitale eye unit.

Discussion: We aim to evaluate a M-health system (Peek CEH) geared towards reducing avoidable blindness through early identification and improved adherence to referral for those with eye problems and reducing demand at secondary care for conditions that can be managed effectively at primary care level.

Trial registration The Pan African Clinical Trials Registry (PACTR), 201807329096632. Registered 8th June 2018, https://pactr.samrc.ac.za
Effects of dexmedetomidine on porcine pulmonary artery vascular smooth muscle
Authors: Sato Kenichi , Chikuda Mami 
Published: July 2019

Abstract
Background: The α2-receptor agonists, dexmedetomidine (Dex) have been shown to produce sedative and analgesic effects not only with systemic administration but also when administered in the extradural space and around peripheral nerves. However, the effects and mechanism of action of Dex on pulmonary arteries have not been determined. This study therefore aimed to investigate the effect of Dex on pulmonary arterial vascular smooth muscle by evaluating changes in isometric contraction tension. We then attempted to determine the effects of Dex on depolarization stimulation and receptor stimulation.
Methods: Endothelium-denuded porcine pulmonary arteries were sliced into 2- to 3-mm rings. We then exposed them to various substances at various concentrations under different conditions of baseline stimulation (with KCl, adrenaline, caffeine, or histamine) and of the α2-receptor stimulant or antagonists, or α1-receptor antagonist (with imidazoline, yohimbine, rauwolscine, or prazosin), and different conditions of Ca2+ depletion of the intracellular reservoir or extracellular stores, measuring the changes in isometric contraction tension with each addition or change in conditions. The concentration–response relation was determined at Dex concentrations of 10−10, 10−9, 10−8, 10−7, 10−6, 5×10−6, and 10−5 M and for other experiments at 5×10-6 M.
Results: Dex enhanced the contraction induced by high KCl stimulation, with the increases reaching significance at Dex concentrations of ≥5×10-6 M. The Dex-induced enhancement of contraction induced by high KCl was completely suppressed by yohimbine and rauwolscine, which are α2-receptor antagonists, but not by prazosin. Dex, imidazoline, yohimbine and rauwolscine reduced the increases in contraction tension induced by the receptor stimulant adrenaline. Dex suppressed the adrenaline-induced increases in contraction tension after depletion of Ca2+ reservoir. In the absence of extracellular Ca2+, Dex suppressed the adrenaline- and histamine-induced increases, and did not affect caffeine-induced increases in contraction tension.
Conclusions: Dex-enhanced high KCl-induced contraction was mediated by α2-receptors. Adrenaline-induced contraction was suppressed by the α2-receptor stimulant Dex and α2-receptor antagonists yohimbine and rauwolscine, suggesting that the effect of Dex on adrenaline-induced contraction is attributable to its α2-receptor-blocking action. Dex inhibited receptor-activated Ca2+ channels (RACCs) and phosphatidylinositol-1,4,5-triphosphate-induced Ca2+ release (IICR) but not Ca2+-induced Ca2+ release (CICR).
COMPREENDENDO A NECESSIDADE DE UM CURSO DE FORMAÇÃO SUPERIOR EM LÍNGUA MACUXI A DISTÂNCIA QUE PODERIA SER OFERTADO PELO INSIKIRAN - UFRR.
Authors: Ferreira Paulo , Machado Ananda 
Published: July 2019

Este estudo tem por interesse compreender a Formação Superior Indígena Multicultural em como este contexto resguarda uma nova demanda atual: a necessidade que o Instituto de Formação Superior Indígena em Roraima – INSIKIRAN, presente na Universidade Federal de Roraima – UFRR, observe a viabilidade de um Curso de Formação Superior em Língua Macuxi a Distância. Visando cumprir esse propósito, baseado em modelo bibliográfico-qualitativo de pesquisa, a abordagem do tema de fundamentou em publicações textos, teses, artigos e dissertações que favorecessem uma melhor compreensão sobre essa realidade. À medida que o tema fora mais bem discutido, se pôde obter uma melhor visão e conhecimento sobre a Formação Superior Indígena Multicultural.
Palavras-chave: Formação Superior. Multiculturalismo. Línguas Indígenas.
A SOCIOLOGIA DE WEBER NO PENSAMENTO DE ELIAS
Authors: Braga de Faria Júnior Walmir José 
Published: July 2019

Com este artigo, objetivamos analisar como certas sugestões de um autor considerado como um clássico da teoria sociológica inspiraram a obra e o pensamento do sociólogo alemão Norbert Elias (1897-1990). No caso, nos concentraremos nas ideias e no referencial de Max Weber (1864-1920). Dessa forma, será descrito como a contribuição do clássico foi retomada pelo autor contemporâneo em dimensões como a união e/ou diálogo entre distintas disciplinas, a compatibilização entre explicar e compreender, e a abordagem de processos de longa duração. Assim, o argumento que perpassa este escrito é que a contribuição do clássico é central para a tarefa de se entender o percurso das ideias do autor contemporâneo, havendo uma articulação criativa, embora nem sempre evidente, das sugestões daquele teórico no cerne da sociologia renovadora praticada por Elias. Para azeitar essa discussão, lançaremos algumas reflexões, em sua maior parte inclinadas a argumentar sobre como a sociologia dos clássicos da sociologia, assim como da sua recepção – além de auxiliarem na compreensão mais sofisticada de contextos intelectuais pontuais, ou de processos sociais mais amplos –, podem ser estratégicas para se melhor recuperar o valor heurístico das contribuições dos clássicos, e até mesmo dos sociólogos contemporâneos.
Palavras-chave: Norbert Elias. Max Weber. Clássicos da Sociologia. Sociologia do Conhecimento.
MICHEL FOUCAULT E O PROBLEMA DA RACIONALIDADE
Authors: Freire Alyson Thiago Fernandes 
Published: July 2019

O presente artigo objetiva discutir a abordagem foucaultiana das formas de racionalidade enquanto uma importante atualização crítica do problema da racionalização das sociedades modernas. Desse modo, em sua primeira parte, o artigo se concentra nas principais premissas e cautelas metodológicas do uso do conceito de racionalidade no esquema teórico de Michel Foucault. Por último, exploro como o filósofo francês discorre sobre os vínculos históricos entre racionalidade, poder e crítica.
Palavras-chave: Michel Foucault. Racionalidade. Racionalização. Teoria Social
EDITORIAL: Ainda há clássicos que são contemporâneos
Authors: Freitas Carlos , Bueno Arthur 
Published: July 2019

Na presente edição, as leitoras e leitores vão conhecer o Dossiê “TEORIA SOCIAL: Os clássicos nos Contemporâneos”, organizado pelos professores Carlos Freitas (UFRN) e Arthur Bueno (Universidade de Frankfurt/Alemanha). O dossiê tem como proposta geral a publicação de artigos inéditos que examinam como os clássicos das ciências sociais se mostram atuais em programas de pesquisa de sociólogos contemporâneos. De modo geral, o dossiê temático é composto por artigos assinados por nomes cientistas sociais de diferentes instituições de ensino e pesquisa do Brasil (UFRN, IFRN, UFPE, UFPB, UFRGS, UFPR, UnB), do Canadá (Universidade de Sydney) e da Alemanha (Universidade de Frankfurt).
Synthesis, In silico and Biological Studies of Thiazolyl-2h-chromen-2-one Derivatives as Potent Antitubercular Agents
Authors: Jadhav Bhagwat S. , Yamgar Ramesh S. , Kenny Rajesh S. , Mali Suraj N. , Chaudhari Hemchandra K. , Mandewale Mustapha C. 
Published: July 2019

Background:
A series of new six thiazolyl-2-amine-based Schiff base derivatives (4a-4f) were
synthesized by a sequential multistep reaction starting with Salicylaldehyde.

Methods:
All the Schiff base derivatives were screened in-vitro for their antibacterial activity against Mycobacterium
tuberculosis (H37RV strain) ATCC No-27294. The synthesized compounds were characterized
by FTIR, 1H-NMR, 13C-NMR and Mass spectrometry.

Results:
Among the compounds tested, 4c and 4f derivatives exhibited potent antitubercular activity
against M. tuberculosis at MIC 6.25 μg/mL.

Conclusion:
We extended our study to explore the inhibition mechanism by conducting molecular docking
analysis by using Schrodinger’s molecular modeling software. All the newly synthesized compounds were
found to be in-silico AMES test non-toxic and non-carcinogens. The good Qikprop’s Absorption, Distribution,
Metabolism and Excretion (ADMET) would definitely help the researchers in order to make more potent
Anti-TB agents.
Synthesis, Antimicrobial and Antitumor Evaluations of a New Class of Thiazoles Substituted on the Chromene Scaffold
Authors: Elnaggar Dina H. , Abdel Hafez Naglaa A. , Rashdan Huda R.M. , Abdelwahed Nayera A.M. , Awad Hanem M. , Ali Korany A. 
Published: July 2019

Background & Objective:A new series of thiazoles substituted on the chromene scaffold were prepared by facial approaches starting from (E)-1-(2,3-Dihydrochromen-4-ylidene)thiosemicarbazide derivatives (2a,b). The thiosemicarbazides (2a,b) were reacted with a series of α-halo carbonyl compounds to give the corresponding rhodanine analogues and reacted also with C-acetyl-or Cethoxy- N-hydrazonoyl chlorides to afford the corresponding tri- and tetra-substituted hybrid hydrazinyl thiazole substituted chromenes.Methods:The newly synthesized compounds were screened for their in vitro antimicrobial and antitumor activities by agar diffusion method and MTT assay, respectively.Results:The results of the antimicrobial activity revealed that some of the new compounds exhibited excellent activity against pathogenic microorganism; Candida albicans compared with Ciprofloxacin and nystatin, as the reference drugs.:All of the tested compounds exhibited significant cytotoxic activities comparable to that of the reference drug; Doxorubicin® (on HCT116 (colorectal carcinoma human cell line).
Sam68 Promotes the Progression of Human Breast Cancer through inducing Activation of EphA3
Authors: Chen Xinxin , Zhang Lehong , Yuan Min , Kuang Ziqiao , Zou Ying , Tang Tian , Zhang Wangjian , Hu Xiaowu , Xia Ting , Cao Tengfei , Jia Haixia 
Published: July 2019

Background:
Src associated with mitosis of 68 kDa (Sam68), is often highly expressed in
human cancers. Overexpression of Sam68 has been shown to be correlated with poor survival prognosis
in some cancer patients. However, little is known whether Sam68 plays a role in promoting
metastasis in breast cancer.

Materials and Methods:
The expression of Sam68 protein in breast cancer tissue was detected by
immunohistochemistry. Trans-well assay, wound-healing, real-time PCR and Western blotting
analysis were used to detect the effect of Sam68 on promoting EMT or metastasis of breast cancer.
Next-generation RNA sequencing was used to analyze genes that may be regulated by Sam68.

Results:
Sam68 plays a positive role in promoting breast cancer metastasis. Sam68 was found to be
overexpressed in breast cancer along with lymph node metastasis. MMP-9 was also found to be
overexpressed in breast cancer tissue and was correlated to the expression of Sam68 (P<0.01).
Xenograft in NOD/SCID mice and in vitro experiments confirmed that the invasion and metastatic
ability of breast cancer cells were regulated by Sam68. And EPHA3 could be up-regulated by
Sam68 in breast cancer.

Conclusion:
High expression of Sam68 participates in breast cancer metastasis by up-regulating the
EPHA3 gene.
Utilization of the Plant Clusia Fluminensis Planch & Triana Against Some Toxic Activities of the Venom of Bothrops jararaca and B. jararacussu Snake Venom Toxic Activities
Authors: da Silva Aldo Rodrigues , Anholeti Maria Carolina , Pietroluongo Marcia , Sanchez Eladio Flores , Valverde Alessandra Leda , de Paiva Selma Ribeiro , Figueiredo Maria Raquel , Kaplan Maria Auxiliadora Coelho , Fuly André Lopes 
Published: July 2019

Background:In Brazil, the Bothrops genus accounts for 87% of registered snakebites, which are characterized by hemorrhage, tissue necrosis, hemostatic disturbances, and death. The treatment recommended by governments is the administration of specific antivenoms. Although antivenom efficiently prevents venom-induced lethality, it has limited efficacy in terms of preventing local tissue damage. Thus, researchers are seeking alternative therapies able to inhibit the main toxic effects of venoms, without compromising safety.Objective:The study aimed to test the ability of aqueous extracts of leaves, stems, and fruits of the plant Clusia fluminensis to neutralize some toxic effects induced by the venoms of Bothrops jararaca and Bothrops jararacussu.Methods:The plant extracts were incubated with venoms for 30 min. at 25 °C, and then in vitro (coagulant and proteolytic) and in vivo (hemorrhagic, myotoxic, and edematogenic) activities were evaluated. In addition, the extracts were administered to animals (by oral, intravenous or subcutaneous routes) before or after the injection of venom samples, and then hemorrhage and edema assays were performed. In addition, a gel solution of the fruit extract was produced and tested in terms of reducing hemorrhage effects. A chemical prospection was performed to identify the main classes of compounds present in the extracts.Results:All the extracts inhibited the activities of the two venoms, regardless of the experimental protocol or route of administration of the extracts. Moreover, the gel of the fruit extract inhibited the venom-induced-hemorrhage. The extracts comprised of tannins, flavonoids, saponins, steroids, and terpenoids.Conclusion:Antivenom properties of C. fluminensis extracts deserve further investigation in order to gain detailed knowledge regarding the neutralization profile of these extracts.
The Possible Role of Telomere Length and Chemokines in the Aging Process: A Transdiagnostic Review in Psychiatry
Authors: Valiati Fernanda Endler , Hizo Gabriel Henrique , Pinto Jairo Vinícius , Kauer-Sant`Anna Márcia 
Published: July 2019

Background:Psychiatric disorders are common, reaching a worldwide prevalence of 29.2%. They are associated with a high risk of premature death and with accelerated aging in clinical, molecular and neuroimaging studies. Recently, there is strong evidence suggesting a possible role of telomere length and chemokines in aging processes in psychiatric disorders.Objective:We aimed to review the literature on telomere length and chemokines and its association with early aging in mental illnesses on a transdiagnostic approach.Results:The review highlights the association between psychiatric disorders and early aging. Several independent studies have reported shorter telomere length and dysregulations on levels of circulating chemokines in schizophrenia, bipolar disorder, major depressive disorder, and anxiety disorders, suggesting a complex interaction between these markers in a transdiagnostic level. However, studies have investigated the inflammatory markers and telomere shortening separately and associated with a particular diagnosis, rather than as a transdiagnostic biological feature.Conclusion:There is consistent evidence supporting the relationship between accelerated aging, telomere length, and chemokines in mental disorders, but they have been studied individually. Thus, more research is needed to improve the knowledge of accelerated senescence and its biomarkers in psychiatry, not only individually in each diagnosis, but also based on a transdiagnostic perspective. Moreover, further research should try to elucidate how the intricate association between the chemokines and telomeres together may contribute to the aging process in psychiatric disorders.
New Anti-inflammatory Triterpene Esters and Glycosides from Alstonia scholaris
Authors: Sultana Nighat , Qazi Muhammad Saleem , Kamal Mustafa 
Published: July 2019

Background::
Phytochemical studies on the ethanolic extract of aerial parts of
Alstonia scholaris lead to the isolation of two new triterpenoid of the lanostanetype, lanosta
5ene,24-ethyl-3-O-β-D-glucopyranoside (1), lanosta,5ene,24-ethyl-3-O-β-D-glucopyranosideester
(2) and new ursane type triterpenoidmethylester, 12-ursene-2,3,18,19-tetrol,28
acetate (nighascholarene) (3), together with seven known triterpenes, betuline, triterpene of
the lupane type, alstoprenyol (4), 3β-hydroxy-28-β-acetoxy-5-olea triterpene (5),α-amyrin
acetate (6), α-amyrin (7), lupeol acetate (8), 3β-hydroxy-24-nor-urs-4,12,28-triene triterpene
(9) and ursolic acid (l0).

Methodology::
The triterpenoid structures of these colorless compounds were deduced from
the 1H and 13C-NMR data, and in particular from the application of two-dimensional 1H,
13C correlation experiments as well as by comparison with reported literature data.

Results and Conclusion:
This study deals with isolation and structural elucidation of natural
new triterpenoidesters and glycosides with anti-inflammatory activity.