bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Vitro Study of the Interaction Between HSA and 4‐Bromoindolylchalcone, a Potent Human MAO‐B Inhibitor: Spectroscopic and Molecular Modeling Studies
Authors: Chaves Otávio Augusto , Sasidharan Rani , dos Santos de Oliveira Cosme H. C. , Manju Sreedharannair Leelabaiamma , Joy Monu , Mathew Bijo , Netto‐Ferreira José Carlos 
Published: January 2019

AbstractBased on our previous report, (2E)‐3‐(4‐bromophenyl)‐1‐(1H‐indol‐3‐yl) prop‐2‐en‐1‐one (IC9) showed potent and reversible hMAO−B inhibitor with Ki=0.010±0.005 μM and a selectivity index of 120 ‐ better than selegiline, the standard drug for hMAO−B. To continue the pharmacological investigation of IC9, the present study describes in vitro interaction between the titled compound and human serum albumin (HSA) under physiological condition by spectroscopic techniques (UV‐Vis, circular dichroism, steady‐state, synchronous, 3D and time‐resolved fluorescence) combined with molecular docking and quantum chemical calculations. There is a moderate ground‐state association between HSA:IC9, which is enthalpically and entropically driven. This association occurs mainly inside Sudlow's site I. There is a weak perturbation on the secondary structure and on the microenvironment around Trp residue as evidenced by circular dichroism and synchronous fluorescence. Molecular docking suggested that IC9 can interact via hydrogen bonding, hydrophobic and electrostatic forces, whereas quantum chemical calculations suggested that the presence of a bromine atom is supporting the ability of binding between IC9 and HSA through an electrostatic interaction.
Hybrid Anodic and Metal‐Assisted Chemical Etching Method Enabling Fabrication of Silicon Carbide Nanowires
Authors: Chen Yun , Zhang Cheng , Li Liyi , Zhou Shuang , Chen Xin , Gao Jian , Zhao Ni , Wong Ching‐Ping 
Published: January 2019

AbstractSilicon carbide (SiC) is one of the most important third‐generation semiconductor materials. However, the chemical robustness of SiC makes it very difficult to process, and only very limited methods are available to fabricate nanostructures on SiC. In this work, a hybrid anodic and metal‐assisted chemical etching (MACE) method is proposed to fabricate SiC nanowires based on wet etching approaches at room temperature and under atmospheric pressure. Through investigations of the etching mechanism and optimal etching conditions, it is found that the metal component plays at least two key roles in the process, i.e., acting as a catalyst to produce hole carriers and introducing band bending in SiC to accumulate sufficient holes for etching. Through the combined anodic and MACE process the required electrical bias is greatly lowered (3.5 V for etching SiC and 7.5 V for creating SiC nanowires) while enhancing the etching efficiency. Furthermore, it is demonstrated that by tuning the etching electrical bias and time, various nanostructures can be obtained and the diameters of the obtained pores and nanowires can range from tens to hundreds of nanometers. This facile method may provide a feasible and economical way to fabricate SiC nanowires and nanostructures for broad applications.
Mouse Wnt1-CRE-RosaTomato Dental Pulp Stem Cells Directly Contribute to the Calvarial Bone Regeneration Process
Authors: Collignon Anne-Margaux , Castillo-Dali Gabriel , Gomez Eduardo , Guilbert Thomas , Lesieur Julie , Nicoletti Antonino , Acuna-Mendoza Soledad , Letourneur Didier , Chaussain Catherine , Rochefort Gael Y. , Poliard Anne 
Published: January 2019

Abstract
Stem cells endowed with skeletogenic potentials seeded in specific scaffolds are considered attractive tissue engineering strategies for treating large bone defects. In the context of craniofacial bone, mesenchymal stromal/stem cells derived from the dental pulp (DPSCs) have demonstrated significant osteogenic properties. Their neural crest embryonic origin further makes them a potential accessible therapeutic tool to repair craniofacial bone. The stem cells’ direct involvement in the repair process versus a paracrine effect is however still discussed. To clarify this question, we have followed the fate of fluorescent murine DPSCs derived from PN3 Wnt1-CRE- RosaTomato mouse molar (T-mDPSCs) during the repair process of calvaria bone defects. Two symmetrical critical defects created on each parietal region were filled with (a) dense collagen scaffolds seeded with T-mDPSCs, (b) noncellularized scaffolds, or (c) no scaffold. Mice were imaged over a 3-month period by microcomputed tomography to evaluate the extent of repair and by biphotonic microscopy to track T-mDPSCs. Histological and immunocytochemical analyses were performed in parallel to characterize the nature of the repaired tissue. We show that T-mDPSCs are present up to 3 months postimplantation in the healing defect and that they rapidly differentiate in chondrocyte-like cells expressing all the expected characteristic markers. T-mDPSCs further maturate into hypertrophic chondrocytes and likely signal to host progenitors that form new bone tissue. This demonstrates that implanted T-mDPSCs are able to survive in the defect microenvironment and to participate directly in repair via an endochondral bone ossification-like process. Stem Cells 2019;37:701–711
Optimization of cyclopropane fatty acids production in Yarrowia lipolytica
Authors: Czerwiec Quentin , Idrissitaghki Abdelghani , Imatoukene Nabila , Nonus Maurice , Thomasset Brigitte , Nicaud Jean‐Marc , Rossignol Tristan 
Published: January 2019

AbstractCyclopropane fatty acids, which can be simply converted to methylated fatty acids, are good unusual fatty acid candidates for long‐term resistance to oxidization and low‐temperature fluidity useful for oleochemistry and biofuels. Cyclopropane fatty acids are present in low amounts in plants or bacteria. In order to develop a process for large‐scale biolipid production, we expressed 10 cyclopropane fatty acid synthases from various organisms in the oleaginous yeast Yarrowia lipolytica, a model yeast for lipid metabolism and naturally capable of producing large amounts of lipids.The Escherichia coli cyclopropane fatty acid synthase expression in Y. lipolytica allows the production of two classes of cyclopropane fatty acids, a C17:0 cyclopropanated form and a C19:0 cyclopropanated form, whereas others produce only the C17:0 form. Expression optimization and fed‐batch fermentation set‐up enable us to reach a specific productivity of 0.032 g·L−1·hr−1 with a genetically modified strain containing cyclopropane fatty acid up to 45% of the total lipid content corresponding to a titre of 2.3 ± 0.2 g/L and a yield of 56.2 ± 4.4 mg/g.
Taking Account of Land-Sea Interactions in Marine Spatial Planning
Authors: Kidd Sue , Jones Hannah , Jay Stephen 
Published: January 2019

AbstractThis chapter reflects on marine/maritime spatial planning (MSP) from a Land-Sea Interaction (LSI) perspective. It raises questions about the role and limitations of MSP in addressing sustainable development of the world’s oceans as many of the issues it is concerned with are inextricably connected to activity on the land. The chapter starts by identifying LSI considerations in some of the key documents that are guiding the establishment of MSP. It then sets out a framework for considering LSI and explores the different dimensions with particular reference to examples from European experience. It ends with an exploration of how LSI matters might inform future directions for MSP and may be heralding a new era of Territorial Spatial Planning (TSP), which spans both land and sea.
Scenario-Building for Marine Spatial Planning
Authors: McGowan Lynne , Jay Stephen , Kidd Sue 
Published: January 2019

AbstractThis chapter focuses on the use of scenarios to inform the development of marine spatial plans. An in-depth example of scenario-building for the Celtic Seas is presented, based on a four-way possibility space using the axes of spatial efficiency and cooperation between planning authorities to create four scenarios which were tested in a workshop. This demonstrated the importance of using scenarios to understand the aspirations of different stakeholders towards integration within the marine spatial planning (MSP) process, the realities of encouraging co-location between sea uses and highlighted important issues where further transboundary cooperation on MSP may be required.
The Need for Marine Spatial Planning in Areas Beyond National Jurisdiction
Authors: Altvater Susanne , Fletcher Ruth , Passarello Cristian 
Published: January 2019

AbstractMarine Spatial Planning (MSP) provides an important tool for sustainably managing mounting pressures on the living and non-living resources in marine areas beyond national jurisdiction (ABNJ). Taking into account the interests of sectors and nature conservation, MSP fosters processes related to stakeholder exchange and collective governance mechanisms. A large amount of experience has been gained at national or regional level on how to apply MSP in cross-border situations. This chapter highlights the existing legal framework and which organisations could foster MSP in ABNJ. Furthermore, it assesses whether existing MSP tools are somehow transferable to ABNJ and shows first results of MSP projects in these areas.
Socio-cultural Dimensions of Marine Spatial Planning
Authors: McKinley Emma , Acott Tim , Stojanovic Tim 
Published: January 2019

AbstractBringing together the complex social and cultural dimensions of marine spatial planning (MSP), this chapter examines how these two terms are applied (or not) in the context of MSP. Global marine and coastal planning continues to recognise that human activities must be considered in order to account for the dynamic interconnectivity between society and the sea. Many research fields explore the importance of the sea to identity, sense of place, health or community cohesion. However, these draw on a range of different assumptions to mainstream marine science and struggle to be incorporated into traditional policy processes, environmental assessments and large-scale planning processes. In this chapter, we interrogate the concept of ‘socio-cultural’, examining how this is being defined and applied across the MSP landscape.
Politics and Power in Marine Spatial Planning
Authors: Flannery Wesley , Clarke Jane , McAteer Benedict 
Published: January 2019

AbstractMarine spatial planning (MSP) has been lauded as a remedy to unsuitable marine management. There is, however, growing MSP research illustrating that it is failing to foster paradigm shifts towards sustainable governance. The gap between MSP theory and practice is due to its asocial and apolitical implementation. This narrow version of MSP has been advanced through post-political planning and uncritical rationalities. The result is a choreographed form of MSP, with clearly defined outcomes that serve the needs of elite actors rather than the public interest. This chapter argues that to recapture its democratising potential, MSP requires explicit engagement with politics and power. We highlight the use of the boundary object lens and citizen science as two potential avenues to facilitate this engagement.
Out-Migration from Peripheries: How Cumulated Individual Strategies Affect Local Development Capacities
Authors: Moldovan Aura 
Published: January 2019

AbstractIn light of the increasing polarisation affecting Central and Eastern Europe over the past two and a half decades, peripheries have been struggling with a range of economic and demographic issues, including selective out-migration. Using peripheries from Romania’s Sălaj County as a case study, the chapter draws on structured interviews with locals and public officials in order to: (1) identify how locals utilise different forms of mobility as strategies to cope with structural deficits aggravated by peripheralisation; and (2) highlight the impact of selective out-migration on the diminishing local development capacities of public officials in peripheries. Through this actor-based approach, the analysis can broadly identify the development strategies already employed in peripheries and what effect they truly have on decreasing core–periphery inequalities.
Leading Through Image Making? On the Limits of Emphasising Agency in Structurally Disadvantaged Rural Places
Authors: Plüschke-Altof Bianka , Grootens Martiene 
Published: January 2019

AbstractAs one response to peripheralisation, agency-based concepts like place leadership and image making have received increasing attention in research on regional polarisation. Based on fieldwork in Estonian rural areas between 2015 and 2017, this chapter sheds light on unexpected challenges faced by local leaders applying these solutions and researchers trying to make sense of them. While place leadership and image making can play a crucial role in fighting regional polarisation, it is shown that they also create new problems of idealisation and responsibilisation in structurally disadvantaged rural areas. Through a discussion of the limits of these response strategies, the chapter reflects upon the complexity that practitioners and researchers experience when acting in these places, which also questions the recent focus on agency prevalent in research on regional polarisation.
Questioning the Convergence of Cohesion and Innovation Policies in Central and Eastern Europe
Authors: Loewen Bradley , Schulz Sebastian 
Published: January 2019

AbstractIn the arena of EU regional development, Cohesion Policy and Innovation Policy are converging into a common economic strategy. Nevertheless, a scholarly debate surrounding the theoretical incompatibilities between the two is especially relevant to Central and Eastern Europe (CEE), where the majority of regions are beneficiaries of Cohesion Policy while attempting to implement innovation strategies. Based on a multi-national comparative analysis of policy documents and expert interviews from Czechia, Estonia, Hungary and Slovakia, this chapter assesses how the policy areas are interrelated, asking whether they should be seen as furthering regional polarisation instead of territorial cohesion. Acknowledging differences in national responses, it is necessary to further articulate, and possibly separate, the objectives of the respective policies, in order to protect the integrity of cohesion initiatives.
Divergent Paths to Cohesion: The (Unintended) Consequences of a Place-Based Cohesion Policy
Authors: Telle Stefan , Špaček Martin , Crăciun Daniela 
Published: January 2019

AbstractThis chapter employs computer-assisted text analysis (CATA) to measure changes in EU Cohesion Policy (CP) objectives and to illustrate their place-based consequences. The method confirms a major shift in focus from employment and social objectives under the Lisbon Agenda to growth and innovation objectives under the Europe 2020 strategy. At the regional level, the same tendency has been found in German and Czech cross-border cooperation programmes in the last two programming periods. However, a more in-depth analysis reveals that these changes are significantly more pronounced in older member states. The chapter interprets its findings as evidence for a divergence between an innovation-driven path to cohesion in older member states and an inclusion-driven path to cohesion in the new member states and offer some tentative explanations.
Preoperative Leucocyte‐Based Inflammatory Scores in Patients with Colorectal Liver Metastases: Can We Count on Them?
Authors: Dupré Aurélien , Jones Robert P. , Diaz‐Nieto Rafael , Fenwick Stephen W. , Poston Graeme J. , Malik Hassan Z. 
Published: January 2019

AbstractBackgroundNeutrophil‐to‐lymphocyte ratio (NLR), platelet‐to‐lymphocyte ratio (PLR) and lymphocyte‐to‐monocyte ratio (LMR) have been identified as potential prognostic factors for overall survival (OS) in primary colorectal cancer, and there is a growing interest in their use in colorectal liver metastases (CLMs). However, optimal cut‐off values for these ratios have not been defined by making comparison between series difficult. This study aimed to confirm the prognostic value of inflammatory scores in patients undergoing resection for CLM.
MethodsWe retrospectively analysed data from 376 consecutive patients who underwent liver surgery for CLM between June 2010 and August 2015. We assessed the reproducibility of previously published ratios and determined new cut‐off values using the Cut‐off Finder web‐based tool. Relations between cut‐off values and OS were analysed with Kaplan–Meier log‐rank survival analysis and multivariate Cox models.ResultsThree hundred and forty‐three patients had full preoperative blood tests for calculation of NLR, PLR and LMR. The number of cut‐off values which showed a significant discrimination for OS was 49/249 (19.7%) for NLR, 28/316 (8.9%) for PLR and 22/214 (10.3%) for LMR, all with a scattered nonlinear distribution.ConclusionsThis study showed that inflammatory scores expressed as ratios do not seem to be consistently reliable prognostic markers in patients with resectable CLM.
High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus
Authors: da Cunha Aureliano Claret , Gomes Lorena Soares , Godoy-Santos Fernanda , Faria-Oliveira Fábio , Teixeira Janaína Aparecida , Sampaio Geraldo Magela Santos , Trópia Maria José Magalhães , Castro Ieso Miranda , Lucas Cândida , Brandão Rogelio Lopes 
Published: January 2019

Abstract
Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.
Refining and expanding nonribosomal peptide synthetase function and mechanism
Authors: McErlean Matt , Overbay Jonathan , Van Lanen Steven 
Published: January 2019

Abstract
Nonribosomal peptide synthetases (NRPSs) are involved in the biosynthesis of numerous peptide and peptide-like natural products that have been exploited in medicine, agriculture, and biotechnology, among other fields. As a consequence, there have been considerable efforts aimed at understanding how NRPSs orchestrate the assembly of these natural products. This review highlights several recent examples that continue to expand upon the fundamental knowledge of NRPS mechanism and includes (1) the discovery of new NRPS substrates and the mechanism by which these sometimes structurally complex substrates are made, (2) the characterization of new NRPS activities and domains that function during the process of peptide assembly, and (3) the various catalytic strategies that are utilized to release the NRPS product. These findings continue to strengthen the predictive power for connecting genes to products, thereby facilitating natural product discovery and development in the Genomics Era.
Entrepreneurship capital spillovers at the local level
Authors: Massón-Guerra José Luis , Ortín-Ángel Pedro 
Published: January 2019

AbstractThe paper analyses three underexplored issues in the entrepreneurship capital spillover literature, namely, its local nature, the generators and the receptors of such spillovers. For that purpose, we take advantage of the Ecuadorian census of establishments. Unlike previous evidence, we can estimate the spillovers at the establishment level, compute the entrepreneurship capital at the local level, and compare different permissiveness levels in the application of registration and tax legislation to businesses (i.e., the relative importance of the informal economy). In general, we find entrepreneurship capital spillovers at the local level. The spillover effects are lower when the entrepreneurship capital has been accumulated in informal businesses. By contrast, informal, large and more technologically developed establishments benefit more from these spillover effects. The paper discusses the implications of those findings for the design of public policies for promoting entrepreneurship.
Closing the mass budget of a tidewater glacier: the example of Kronebreen, Svalbard
Authors: DESCHAMPS-BERGER CESAR , NUTH CHRISTOPHER , VAN PELT WARD , BERTHIER ETIENNE , KOHLER JACK , ALTENA BAS 
Published: January 2019

ABSTRACTIn this study, we combine remote sensing, in situ and model-derived datasets from 1966 to 2014 to calculate the mass-balance components of Kronebreen, a fast-flowing tidewater glacier in Svalbard. For the well-surveyed period 2009–2014, we are able to close the glacier mass budget within the prescribed errors. During these 5 years, the glacier geodetic mass balance was −0.69 ± 0.12 m w.e. a−1, while the mass budget method led to a total mass balance of −0.92 ± 0.16 m w.e. a−1, as a consequence of a strong frontal ablation (−0.78 ± 0.11 m w.e. a−1), and a slightly negative climatic mass balance (−0.14 ± 0.11 m w.e. a−1). The trend towards more negative climatic mass balance between 1966–1990 (+0.20 ± 0.05 m w.e. a−1) and 2009–2014 is not reflected in the geodetic mass balance trend. Therefore, we suspect a reduction in ice-discharge in the most recent period. Yet, these multidecadal changes in ice-discharge cannot be measured from the available observations and thus are only estimated with relatively large errors as a residual of the mass continuity equation. Our study presents the multidecadal evolution of the dynamics and mass balance of a tidewater glacier and illustrates the errors introduced by inferring one unmeasured mass-balance component from the others.