bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reflexiones sobre la Importancia de una Gobernanza Institucional en el cumplimiento del ODS 3 Bienestar y Salud (Agenda 2030)
Authors: Cedeño-Espinel Jean , Miranda-Pichucho Freddy 
Published: November 2024

La investigación tiene como general: Reflexionar sobre la importancia de la gobernanza institucional en el cumplimiento del ODS 3 Bienestar y salud (Agenda 2030); a su además de dos objetivos específicos: Identificar las posturas teóricas sobre gobernanza institucional que dan viabilidad al desarrollo sostenible; y, develar las vertientes establecidas para el alcance del ODS 3 Bienestar y salud (Agenda 2030). El diseño de la metodología ha sido partiendo del paradigma fenomenológico-interpretativo con un enfoque cualitativo y no experimental, configurándose una investigación documental/bibliográfica, se parte de un referente epistémico como lo es la Teoría Fundamentada de la cual se hace uso de procedimientos de matrices axiales, entre las cuales se encuentra la matriz paradigmática, la matriz de que permite identificar el proceso de comparación constante o de saturación teórica (Vinculación de los conceptos con las fases del análisis documental) y la matriz de categorización (Matriz de hallazgos emergentes), encontrándose en esta última una categoría central que recoge todos los elementos que han surgido a lo largo del proceso de análisis comparativo, siendo esta denominada: Gobernanza de la salud. Un aporte a su conceptualización.
Utilización de Kahoot para el Refuerzo Académico en Matemáticas en Primer año de Bachillerato Técnico
Authors: Guamaní-Pallo Luis , Choloquinga-Choloquinga María , Chiquito-Chilan Rosa 
Published: November 2024

Los refuerzos académicos se han convertido en herramientas importantes dentro de los procesos de enseñanza aprendizaje en escuelas y colegios del país, por las falencias que se viene arrastrando a partir de la pandemia. Durante el desarrollo de una clase un docente debe encaminarse hacia el objetivo, al no cumplirse este, el maestro está en la obligación de realizar refuerzos para lograr que los estudiantes se apropien del conocimiento impartido. Según datos del Ministerio de Educación, esta deficiencia se concentra en estudiantes del nivel básico superior y bachillerato, lo que explica la alta demanda de estrategias y actividades enfocadas en el refuerzo académico. Por tal razón, la presente investigación propone la utilización de una herramienta digital (kahoot), como un medio alternativo y metodológico en el refuerzo académico el cual servirá para fortalecer y complementar los vacíos académicos generados durante el proceso escolar, además, se considera como una herramienta de aprendizaje utilizada con diferentes finalidades; que permita desarrollar de manera dinámica los programas de refuerzo académico en estudiantes del bachillerato técnico. La institución objeto de investigación es la Unidad Educativa Comunitaria Intercultural Bilingüe “Jatari Unancha”, que cuenta con cuatro figuras profesionales con un aproximado de 1200 estudiantes donde se ha identificado la necesidad de implementar diferentes estrategias innovadoras para promover el desarrollo cognitivo de estudiantes que les permita alcanzar los niveles óptimos en comprensión de las asignaturas especialmente matemáticas, ya que dicha asignatura es de vital importancia para el desarrollo integral del estudiante.
Survival, treatment duration and costs of patients with prostate cancer treated with triptorelin in Italy: a study of administrative databases
Authors: Caffo Orazio , Facchini Gaetano , Degli Esposti Luca , Acciai Valentina , Mauri Giorgio , Mazzanti Paola , Fornarini Giuseppe 
Published: November 2024

Background: Several data support the efficacy/effectiveness, safety and favorable impact on quality of life of triptorelin treatment in patients with prostate cancer. However, little evidence is available concerning triptorelin use in the long term.
Methods: We analyzed data on triptorelin treatment in patients with prostate cancer in an integrated Italian administrative database, covering around 6 million health-assisted subjects throughout the country. Patients with at least one prescription for triptorelin in the period 2010-2020 and with no evidence of metastasis were included and followed up until 2021. Overall survival (OS) and duration of treatment were analyzed using Kaplan-Meier curves, starting from the date of first prescription.
Results: The cohort included a total of 3,411 patients (mean age: 76.8 ± 8.7 years), of whom 1,326 (38.9%) were treated with triptorelin only and 2,085 (61.1%) with triptorelin combined with an anti-androgen. Overall, 847 (24.8%) patients with prostate cancer died and 1,037 (30.4%) had a treatment switch during the follow-up period, and both the median OS and median duration of treatment were not reached in both groups. The mean annual total cost per patient was estimated as 5,574 €, with almost half of the costs related to medication expenses (2,737 €).
Conclusions: We found a long survival and duration of triptorelin treatment in this population of Italian patients with prostate cancer. This study with a long follow-up period further highlights the usefulness of healthcare utilization databases to integrate results obtained from clinical studies with those from everyday clinical practice.
Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids
Authors: Alsehli Haneen S. , Roy Errin , Williams Thomas , Kuziola Alicja , Guo Yunzhe , Dreiss Cecile A. , Green Jeremy B.A. , Gentleman Eileen , Danovi Davide 
Published: November 2024

IntroductionCell lineage specification is tightly associated with profound morphological changes in the developing human embryo, particularly during gastrulation. The interplay between mechanical forces and biochemical signals is poorly understood.MethodsHere, we dissect the effects of biochemical cues and physical confinement on a 3D in vitro model based on spheroids formed from human induced pluripotent stem cells (hiPSCs).ResultsFirst, we compare self-renewing versus differentiating media conditions in free-floating cultures and observe the emergence of tri-germ layers. In these unconfined conditions, BMP4 exposure induces polarised expression of SOX17 in conjunction with spheroid elongation. We then physically confine spheroids using PEG-peptide hydrogels and observe dramatically reduced SOX17 expression, albeit rescued if gels that soften over time are used instead.DiscussionOur study combines high-content imaging, synthetic hydrogels, and hiPSCs-derived models of early development to define the drivers that cause changes in the shape and the emergence of germ layers.
Amino-functionalized vertically ordered mesoporous silica film on electrochemically polarized screen-printed carbon electrodes for the construction of gated electrochemical aptasensors and sensitive detection of carcinoembryonic antigens
Authors: He Ke , Wang Hongxin , Luo Tao , Yan Fei , Guo Jing 
Published: November 2024

Disposable electrochemical biosensors with high sensitivity are very fit for point-of-care testing in clinical diagnosis. Herein, amino-functionalized, vertically ordered mesoporous silica films (NH2-VMSF) attached to an electrochemically polarized screen-printed carbon electrode (p-SPCE) are prepared using a simple electrochemical method and then utilized to construct a gated electrochemical aptasensor for rapid and sensitive determination of carcinoembryonic antigen (CEA). After being treated with the electrochemical polarization procedure, p-SPCE has plentiful oxygen-containing groups and improved catalytic ability, which help promote the stability of NH2-VMSF on SPCE without the use of an adhesive layer and simultaneously generate a highly electroactive sensing interface. Owing to the numerous uniform and ultrasmall nanopores of NH2-VMSF, CEA-specific aptamer anchored on the external surface of NH2-VMSF/p-SPCE serves as the gatekeeper, allowing the specific recognition and binding of CEA and eventually impeding the ingress of electrochemical probes [Fe(CN)63−/4−] through the silica nanochannels. The declined electrochemical responses of Fe(CN)63−/4− can be used to quantitatively detect CEA, yielding a wide detection range (100 fg/mL to 100 ng/mL) and a low limit of detection (24 fg/mL). Moreover, the proposed NH2-VMSF/p-SPCE-based electrochemical aptasensor can be applied to detect the amount of CEA in spiked human serum samples, which extends the biological application of a disposable NH2-VMSF/p-SPCE sensor by modulating the biological recognition species.
Impact of endogenous viral elements on glioma clinical phenotypes by inducing OCT4 in the host
Authors: Li Shirong , He Shuai , Xue Haoyu , He Yi 
Published: November 2024

IntroductionEndogenous viral elements (EVEs) are viral sequences integrated within the host genome that can influence gene regulation and tumor development. While EVEs have been implicated in cancer, their role in regulating key transcription factors in glioblastoma (GBM) remains underexplored. This study investigates the relationship between EVEs and the activation of OCT4, a critical transcription factor in GBM progression.MethodsWe utilized CancerHERVdb and HervD Atlas databases to identify potential interactions between EVEs and key genes involved in GBM. Data from 273 GBM patient samples in the TCGA database were analyzed to examine the correlation between OCT4 expression and mutations in glioma-related genes. Furthermore, glioblastoma stem cells (GSCs) were assessed for the expression levels of OCT4 and SOX2, and Pearson correlation analysis was performed.ResultsOur analysis revealed that OCT4 is a pivotal gene activated by EVEs in GBM. OCT4 expression was significantly correlated with mutations in key glioma-associated genes. Higher OCT4 levels were associated with poorer patient prognosis, higher tumor grades, and older age. Additionally, GSCs exhibited elevated expression of both OCT4 and SOX2, with a positive correlation observed between these two genes in GBM patients.DiscussionThis study highlights the potential role of EVEs in driving GBM progression through the activation of OCT4. The findings emphasize the importance of OCT4 in GBM malignancy and suggest that targeting EVE-mediated pathways may provide new therapeutic approaches for GBM treatment.
Euthyroid sick syndrome predicts the risk of ischemic stroke-associated pneumonia in the acute stage of ischemic stroke: a nested case-control study
Authors: Yu Shuai , Yan Jia , Logan Robert , Tang Wei-Ting , Ye Jun-Nan , Feng Hong-Xuan , Wang Mei-Xia , Xu Qin-Rong , Jiang Xu-Li , Lin Hai-Yan , Wu Guan-Hui , Gui Qian , Duan Ting-Ting 
Published: November 2024

ObjectiveIschemic stroke-associated pneumonia (iSAP) affects about 10% of acute ischemic stroke patients during hospitalization. Current prediction scales for iSAP are insufficient. Identifying early biomarkers for stroke-associated pneumonia is crucial for improving patient outcomes. This study aimed to investigate the predictive value of euthyroid sick syndrome (ESS) for iSAP in acute-stage of ischemic stroke patients.MethodsWe studied 1767 acute ischemic stroke patients within one week of symptom onset, categorizing them into an infection group (iSAP, n=376) and control group (control, n=1391). COX regression analysis was used to identify the potential risk and protected factors. Kaplan-Meier time-event curves and Log-Rank tests were performed to differentiate infection time in patients with ESS or normal T3 group.ResultsThe iSAP group had higher rates of risk factors like older age, atrial fibrillation, COPD, and ESS, along with elevated levels of WBC, CRP,and FT4 levels (all P &lt; 0.001). Conversely, iSAP patients had lower GCS scores, eGFR, TSH, T3, FT3 (all P &lt; 0.001) and T4 levels (P = 0.005) upon admission. No significant differences were observed in sex, smoking history, hypertension, diabetes, or LDL-C levels (P &gt; 0.05). COX regression analysis identified age, KWST scores, leukocyte count, CRP, and ESS (all P &lt; 0.001) as significantly correlated with iSAP. ROC analysis revealed ESS as a predictor with sensitivity of 35.64% and specificity of 87.92% for SAP prediction, like atrial fibrillation and higher than COPD and eGFR.ConclusionESS at admission predicts a higher risk of stroke-associated pneumonia in acute-stage of ischemic stroke.
Gene expression-based modeling of overall survival in Black or African American patients with lung adenocarcinoma
Authors: Zhu Bin , McHale Stephanie S. , Van Scoyk Michelle , Riddick Gregory , Wu Pei-Ying , Chou Chu-Fang , Chen Ching-Yi , Winn Robert A. 
Published: November 2024

IntroductionLung cancer is a leading cause of cancer-related deaths worldwide. Black/African American (B/AA) populations, in particular, exhibit the highest incidence and mortality rates of lung adenocarcinoma (LUAD) in the United States.MethodsThis study aims to explore gene expression patterns linked to LUAD in B/AA and case-matched white patients, with the goal of developing predictive models for prognosis. Leveraging RNA sequencing data from The Cancer Genome Atlas (TCGA) database, genes and pathways associated with overall survival (OS) were identified.ResultsThe OS-associated genes in B/AA patients were distinct from those in white patients, showing predominant enrichment in immune-related pathways. Furthermore, mRNA co-expression network analysis revealed that OS-associated genes in B/AA patients had higher levels of interaction with various pathways, including those related to immunity, cell-ECM interaction, and specific intracellular signaling pathways. Notably, a potential B/AA-specific biomarker, C9orf64, demonstrated significant correlations with genes involved in immune response. Unsupervised machine learning algorithms stratified B/AA patients into groups with distinct survival outcomes, while supervised algorithms demonstrated a higher accuracy in predicting survival for B/AA LUAD patients compared to white patients.DiscussionIn total, this study explored OS-associated genes and pathways specific for B/AA LUAD patients. Further validation and clinical application of these findings are warranted to address disparities and improve outcomes in diverse patient populations.
The association between serum complement 4 and relapse of primary membranous nephropathy: a multicenter retrospective cohort study
Authors: Gan Wenyuan , Zhu Fan , Zeng Xingruo , Xiao Wei , Fang Xun , Su Licong , Chen Wenli 
Published: November 2024

BackgroundRelapse after initial remission reduces renal survival in patients with primary membranous nephropathy (PMN). In this study, we aim to identify risk factors of relapse in PMN and construct a model to identify patients at high risk of relapse early.MethodsWe conducted a multi-center retrospective study using the China Renal Data System database, which includes data from 24 urban academic centers across China. A prediction model based on the Cox proportional hazards model was derived in the derivation group and validated in the validation group.Result515 patients with biopsy-proven PMN achieving initial remission were enrolled. 32.62% of patients subsequently relapsed during a median of 6.08 months. Lower serum albumin (Alb) (per 1 g/L decrease, hazard ratio [HR] =1.48, 95% confidence interval [CI] 1.29–1.78, p &lt; 0.001), lower estimated glomerular filtration rate (eGFR) (per 10 mL/min/1.73m2 decrease, HR =1.14, 95% CI 0.97–1.49, p &lt; 0.001), higher serum complement 4 (C4) (per 0.1 g/L increase, HR =1.89, 95% CI 1.32–3.22, p = 0.012), partial remission (PR) (HR =2.28, 95%CI 1.74–4.04, p &lt; 0.001), and treatment with calcineurin inhibitors (CINs) (HR =1.33, 95%CI 1.04–1.64, p &lt; 0.001) at the time of remission were risk factors for relapse. C-statistic, time-dependent areas under the receiver operating characteristic curve, and calibration plots confirmed that the model had excellent discrimination and calibration in predicting PMN relapse. The anti-phospholipase A2 receptor antibody (aPLA2Rab) titers and pathologic features did not substantially improve the model.ConclusionOur study confirms the well-known low Alb and eGFR, PR, and treatment of CNIs at the time of remission as risk factors for PMN relapse, but aPLA2Rab and pathologic features may not predict relapse. In addition, it is the first study to show serum C4 is associated with PMN relapse. We suggest that complement-targeted therapies may be a potential therapy to prevent PMN relapse.
Empowering primary care physicians in child and adolescent psychiatry: a needs assessment on collaborative care in Dubai
Authors: Berbenyuk Anna , Alameeri Asma , Ismail Asma Bin , Zary Nabil , Sultan Meshal A. 
Published: November 2024

BackgroundChild and adolescent psychiatric disorders pose significant public health concerns necessitating prompt intervention. Primary care physicians (PCPs) play a critical role as initial points of contact, facilitating early detection, management, and referral of these conditions. In Dubai, integrating mental health services into primary care faces unique challenges, highlighting the need for systemic reforms and enhanced PCP training.ObjectiveThis study investigated perceptions, barriers, and systemic challenges encountered by PCPs in managing child and adolescent psychiatric conditions in Dubai’s primary care setting. It also assessed family physicians’ involvement and preparedness in this domain.MethodsUsing a mixed-methods approach, we conducted a survey among family physicians in Dubai Health facilities and analyzed patient data from family medicine clinics. The survey evaluated formal training, preparedness, encounter frequency, and referral patterns for psychiatric care in young patients. Qualitative insights from open-ended survey questions provided additional understanding of specific challenges.ResultsFindings revealed significant gaps in formal training, with only 33.3% of respondents trained in child and adolescent psychiatry during medical education, and 21.1% participating in Continuing Medical Education (CME). A majority (54.4%) of PCPs felt unprepared to manage psychiatric care in young patients. Patient data analysis showed a predominance of male patients (59.9%) and identified Autism Spectrum Disorder (43.1%) as the most common condition, emphasizing the reliance on specialized care through a high referral rate (63.1%). Major barriers included time constraints, limited psychiatric knowledge, resistance to mental health services, systemic and structural issues, communication challenges, and resource shortages.ConclusionEnhanced training and systemic reforms are urgently needed to integrate mental health services effectively into Dubai’s primary care. Implementing structured collaborative care models, fostering interdisciplinary collaboration, and addressing systemic barriers are crucial for improving child and adolescent psychiatric condition management. These initiatives promise better patient outcomes and more efficient healthcare resource utilization.
Bibliometric analysis of research hotspots and trends on the relationship between the gut microbiota and depression from 2020 to 2024
Authors: Xu Dingwen , Wu Jijun , Lu Zhihua , Zhao Xu , Feng Yang , Zhang Weicai , Jiang Shenglu , Zhang Lingling , Wang Ting , Zhao Zhenxiong 
Published: November 2024

IntroductionIn recent years, an increasing body of research has illustrated a strong correlation between gut microbiota and depression. However, there has yet to be a comprehensive discussion or summary of the latest advancements and trends in this field.MethodsWe retrieved research articles focused on gut microbiota and depression through the WOS database from 2020 to 2024, using visual text analysis tools such as CiteSpace and VOSviewer.ResultsThe literature on the relationship between gut microbiota and depression surged from 396 papers in 2020 to 711 by 2024. During this period, the journal with the highest publication rate was Nutrients. China led the countries in contributions, while University College Cork topped the institutions. Kenji Hashimoto emerged as the most prolific author. The most cited paper was authored by Cryan JF et al., published in 2019 in Physiol Rev. The keywords “gut microbiota,” “depression,” and “anxiety” appeared most frequently, while recent years saw explosive increases in terms such as “growth performance,” “receptors,” “depression-like phenotypes,” “stress response,” “gastrointestinal symptoms,” “reliability,” and “neurogenesis.”DiscussionOur article displayed the overview of the relationship between the gut microbiome and depression from 2020 to 2024 using bibliometric methods, providing perspectives and research hotspots for studies exploring the correlation between the gut microbiome and depression.
Proteomics analysis of extracellular vesicles for biomarkers of autism spectrum disorder
Authors: Ali Moussa Houda Yasmine , Shin Kyung Chul , de la Fuente Alberto , Bensmail Ilham , Abdesselem Houari B. , Ponraj Janarthanan , Mansour Said , Al-Shaban Fouad A. , Stanton Lawrence W. , Abdulla Sara A. , Park Yongsoo 
Published: November 2024

BackgroundAutism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by symptoms that include social interaction deficits, language difficulties and restricted, repetitive behavior. Early intervention through medication and behavioral therapy can eliminate some ASD-related symptoms and significantly improve the life-quality of the affected individuals. Currently, the diagnosis of ASD is highly limited.MethodsTo investigate the feasibility of early diagnosis of ASD, we tested extracellular vesicles (EVs) proteins obtained from ASD cases. First, plasma EVs were isolated from healthy controls (HCs) and ASD individuals and were analyzed using proximity extension assay (PEA) technology to quantify 1,196 protein expression level. Second, machine learning analysis and bioinformatic approaches were applied to explore how a combination of EV proteins could serve as biomarkers for ASD diagnosis.ResultsNo significant differences in the EV morphology and EV size distribution between HCs and ASD were observed, but the EV number was slightly lower in ASD plasma. We identified the top five downregulated proteins in plasma EVs isolated from ASD individuals: WW domain-containing protein 2 (WWP2), Heat shock protein 27 (HSP27), C-type lectin domain family 1 member B (CLEC1B), Cluster of differentiation 40 (CD40), and folate receptor alpha (FRalpha). Machine learning analysis and correlation analysis support the idea that these five EV proteins can be potential biomarkers for ASD.ConclusionWe identified the top five downregulated proteins in ASD EVs and examined that a combination of EV proteins could serve as biomarkers for ASD diagnosis.