bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytotoxicity, Antiproliferative and Apoptotic Effect of n-Hexane Fraction of Lime Parasite (Dendrophtoe pentandra)
Authors: Subandrate Subandrate , Diba Masayu Farah , Salni Salni , Triwani Triwani , Nita Sri 
Published: June 2019

Breast cancer is one of the biggest causes of death in women in the world. Lime parasite (Dendrophtoe pentandra (L.) Miq.), a folk remedy used by Indonesian people, is believed to be efficacious as anticancer drug. This research aims to know the activity of n-hexane fractions of lime parasite in inhibiting the proliferation and apoptosis of T47D cells in vitro. Cytotoxic test with MTT method assay from n-hexane fractions used a multilevel concentration. Antiproliferative test was carried out by the method of MTT assay and cell doubling time was calculated at the time of duplication. Apoptotic test was done with concentration of 1 IC50and ½ IC50which was analyzed by flow cytometry. The results reveals that fractions of lime parasite have cytotoxic activity with concentration of IC50is included in moderatecytotoxic level. The result of the doubling time of the optimum fraction of n-hexane is in 31 hours with the concentration of ¼IC50. Results for the flow cytometry shows the fraction of n-hexane does not induce apoptosis in cells of T47D. Those results show that the active fraction of lime parasite has cytotoxic activity which is able to inhibit proliferation, but does not induce apoptosis of T47D cell.
Protective Effect of Ethanol Extract of Celery (Apium graveolens L) on Kidney Damage in Ischemia/ Reperfusion Injury Rats Model
Authors: Afifah Afifah , Muflikhah Khusnul , Ati Viva Ratih Bening , Tsani Rizki Maulana , Khasanah Dairotul , Maulana Watub 
Published: June 2019

Ischemia/ reperfusion injury (IRI) is marked by the sudden decrease of blood supply to the kidney followed by restoration of blood flow. Ischemic acute kidney injury (AKI) is the leading cause of morbidity and mortality. Therefore, the prevention strategy for decreasing the damage due to IRI is required. Celery (Apium graveolens L) is often consumed as food. Celery has antioxidant and anti-inflammatory effects. This study aimed to investigate the protective effect of the celery on kidney damage in the kidney ischemia/ reperfusion injury rat model. Twenty-five rats male, 2-3 months old Sprague Dawley were divided into 5 groups: Group 1 was sham operation, group 2 was ischemia/ reperfusion injury (IRI), group 3, 4, 5 were IRI and 250 mg/kgBW, 500 mg/kgBW, 1000 mg/kgBW of ethanol extract of celery respectively for 14 days before and 3 days after operation. Blood serum sample was taken 3 days after the operation for measuring urea, creatinine, superoxide dismuthase (SOD) and nitrite oxide (NO). Hematoxylin-eosin (HE) staining was utilized to examine kidney tubular injury score. Data were analyzed using One-way ANOVA (p<0,05). The ethanol extract of celery at dose 1000 mg/kgBW prevented the increase of urea, creatinine serum, kidney tubular injury score and prevented the decrease of SOD, NO in the kidney ischemia/ reperfusion injury rat model (p<0,05). In conclusion, the ethanol extract of celery has a protective effect on kidney damage in the ischemia/ reperfusion injury rat model.
A Cytotoxic Compound from n-Hexane Fraction of Lantana camara Linn Leaves
Authors: Suryati Suryati , Malasari Yuni , Efdi Mai , Mardiah Elida 
Published: June 2019

In this study, one triterpenoid compound from n-hexane fraction of Lantana camaraLinn leaves has cytotoxic activity was isolated. Isolation was carried out using gravity chromatographycolumnand purification by recrystallization method. Isolated compound was obtained white solid with melting point 252-253oC. The structure of isolated compound was elucidated using spectroscopic analysis Ultraviolet (UV), Infrared (IR), 1H-Nuclear Magnetic Resonance (1H-NMR), 13C-Nuclear Magnetic Resonance (13C-NMR) Heteronuclear Multiple Bond Connectivity (HMBC), Heteronuclear Multiple Quantum Correlation (HMQC), Distortionless Enhancement Polarization Transfer (DEPT) and comparative literature data, identified as Lantadene A (22β-angeloyloxy-3-oxoolean-12-en-28-oic-acid) with molecule formula C35H52O5. The Lantadene A compound was evaluated for cytotoxic activity against shrimp larvae Artemia SalinaLeach using Brine Shrimp Lethality Test(BSLT) method, showed strong cytotoxic activity with an LC50value of 48.97 µg/mL.
Iatrogenic Hip Instability After Hip Arthroscopy: Is There a Role for Open Capsular Reconstruction?
Authors: Gehrman Max , Cornell Max , Seeley Mark 
Published: June 2019

Case:
A 23-year-old female athlete presented with postoperative hip pain and laxity following 3 arthroscopic hip procedures.

Conclusions:
Open reconstruction of the anterior hip capsule with Achilles allograft can correct arthroscopy-induced hip laxity.
Surgical Reconstruction of an Open Medial Malleolus Fracture Using a Novel Technique
Authors: Kow Ren Yi , Yuen Jin Chuan , Ahmad Alwi Akmal Azim , Abas Muhammad Firdaus , Low Chooi Leng 
Published: June 2019

Case:
A 17-year-old male sustained an open fracture of the right medial malleolus (MM) with significant bone and soft tissue loss following a motor-vehicle accident. Following serial wound debridement, his ankle was effectively reconstructed with MM antiglide plate stabilization, iliac autogenous bone graft, and a free radial forearm soft tissue flap.

Conclusions:
Open MM fracture with bone and soft tissue loss is rare. It is feasible to treat this injury with a novel surgical reconstruction technique involving autogenous bicortical iliac bone graft and radial forearm free flap.
Identifikasi penguasaan konsep fluida statis pada siswa
Authors: Puspita Wanda Indriana , Sutopo Sutopo , Yuliati Lia 
Published: June 2019

Abstract: A good conceptual understanding can help students in solving a problem, because with good conceptual understanding can precisely know the appropriate concept to be used in solving a problem. Static fluid is one of the most complex and fundamental topics in physics learning. However, there are still misconceptions experienced by students regarding this material such as the buoyant force and pressure principle. Therefore, an evaluation of static fluid material for students who have followed this material learning beforehand, to know whether the concept has been precise and meaningful. The method used is qualitative descriptive in the form of qualitative analysis and quantitative analysis to see the percentage of conceptual understanding of 100 students in SMAN 1 Turen. Percentage conceptual understanding on fluid static material can be described as follows, as much as 33,2% student still wrong concept, 40% of student already understood concept and 26,8% student still did not give the reason.

Abstrak: Penguasaan konsep yang baik dapat membantu siswa dalam memecahkan suatu permasalahan, karena dengan penguasaan konsep yang baik dapat secara tepat mengetahui konsep yang sesuai untuk digunakan dalam memecahkan suatu permasalahan. Fluida statis merupakan salah satu topik dalam pembelajaran fisika yang cukup kompleks dan mendasar. Namun masih saja terdapat miskonsepsi yang dialami oleh siswa mengenai materi ini misalnya tentang gaya apung dan prinsip tekanan. Maka dari itu diperlukan evaluasi mengenai materi fluida statis bagi siswa yang telah mengikuti pembelajaran materi ini sebelumnya, untuk mengetahui apakah konsep yang dimilikinya sudah tepat dan bermakna. Metode yang digunakan adalah deskriptif kualitatif berupa analisis kualitatif maupun analisis kuantitatif untuk melihat presentase penguasaan konsep 100 siswa di SMAN 1 Turen. Persentase penguasaan konsep siswa pada materi fluida statis dapat dijabarkan sebagai berikut, sebanyak 33,2% siswa masih salah konsep, 40% siswa sudah paham konsep dan 26,8% siswa masih tidak mengemukakan alasannya.
The analysis of mirror pattern bands synthesized in patients with neurological disorder during Oligoclonal immunoglobulin isoelectric focusing electrophoresis
Authors: Wang Jin Ling , wang peichang 
Published: June 2019

Abstract
Background

The analysis of oligoclonal band (OCB) of cerebrospinal fluid is of vital importance for the clinical diagnosis and differential diagnosis of neurological disease. However, there still exists limited knowledge about the interpretation of the mirror pattern bands (one of OCB results) for the neurological disorders during the clinal courses. In this study, we thoroughly analyzed the 12 patients with mirror pattern bands combined with laboratory and clinical information in order to explore the clinical implications of mirror bands as much as possible.

Methods

Matched CSF and serum specimens were obtained from 5892 patients with various neurological disorders who were required to lumber puncture for OCB analysis over the past four years in the Neurological Special Examination Laboratory of XuanWu Hospital. A total of 12 patients with mirror pattern bands were screened out from the 5892 patients. Serum immunofixation electrophoresis (IFE) was then performed on the 12 patients respectively in order to tentatively explore the correlation with M protein. We also collected the clinical and laboratory information of the 12 patients in great detail.

Results

All three types of mirror patterns are closely connected with M protein from peripheral serum. However, not all patients with the mirror patterns present positive M protein. 7 out of 12 patients (58.3%) were finally diagnosed as blood system-related diseases. None of the 12 patients were diagnosed with Multiple Sclerosis (MS).

Conclusions

Great emphasis should be taken on the mirror pattern bands during the isoelectric focusing electrophoresis (IEF). The mirror pattern bands combined with the result of M protein will greatly contributes to the differential diagnosis of neurological disorder.
The Frequency of Fluoroquinolone Resistant Enterotoxigenic Escherichia coli from Stools of Diarrheic Children in Ebonyi State, Nigeria
Authors: David Ebuka , Yameen MA , Igwenyi IO , Iroha IR , Nzelibe HC , Shuaibu MN , Magaji RA , Onwe OS 
Published: June 2019

Abstract
Enterotoxigenic Escherichia coli (ETEC) is an important cause of acute childhood diarrhea. The evaluation of ETEC in children is important for therapeutic and economic purposes. Hence, this study aimed to evaluate the frequency of ETEC among diarrheic children, their multidrug and fluoroquinolone resistant pattern. A total of twenty diarrheagenic E. coli (DEC) isolates were gotten from hundred diarrheal samples using biochemical and molecular methods. Multiplex PCR was used to detect the presence of four different pathological types of DEC. Disk diffusion method was used to determine the antibiotic susceptibility of the organisms. Biofilm formation was detected by thiazoylblue tetrazolium bromide dye in a 96-well plate. Results showed that ETEC represented 30% of the DEC, of which 80% were multidrug and fluoroquinolone resistant. The biofilm production abilities of all the ETEC were found to exist within weak, moderate and strong biofilm producers. We observed a high ETEC frequency and rapid emergence of multidrug/fluoroquinolone resistance, suggesting that it is one of the most important causes of frequent drug resistant diarrhea in children in this region.
Effects of chitosan coating enriched with thyme essential oil and packaging methods on a postharvest quality of Persian walnut under cold storage
Authors: Talebi Habashi Roghieh , Zomorodi Shahin , Talaie Alireza , Kalateh Jari Sepideh 
Published: June 2019

This study evaluated the effects of edible coatings and different packaging methods on the shelf-life and quality of walnut kernels. It focused on the coatings with chitosan (1%) and thyme essential oil (TEO) at concentra-

tions of 500 and 1,000 μl L–1 (CT

, CT

) or with chitosan alone (CT). The effects of the coatings was assessed

500

1,000

for different packaging methods (LP, loose packaging; PP, packaging in polypropylene bags; and AP, active packa-
ging) as contrasted to control walnuts (C). Walnuts were stored for 120 days in darkness, with relative humidity of 55%, at 4°C. The results showed that the L* index and moisture content of the samples in the chitosan with 500 and 1,000 μl L–1 thyme essential oil in active packaging were maximum, whereas peroxide and conjugated diene values were minimum. The lowest rate of mold growth was observed for the chitosan samples with 500 μl L–1 thyme es- sential oil in active packaging. The best overall acceptability score was related to the samples with chitosan alone and the chitosan with 500 μl L–1 thyme essential oil in active packaging. The chitosan alone and the chitosan with 500 μl L–1 thyme essential oil in active packaging are recommended for storage of kernels at 4°C.
Antipathogenic effects of emulsion and nanoemulsion of cinnamon essential oil against Rhizopus rot and grey mold on strawberry fruits
Authors: Naserzadeh Yousef , Mahmoudi Niloufar , Pakina Elena 
Published: June 2019

Application of essential oils in controlling plant pathogens is generally associated with difficulty due to low solubility in water, strong odor, physical and chemical instability. One of the ways to minimise these effects is to use a nanoemulsion system. It also increases the antimicrobial properties. In this research, after preparation of cinnamon (Cinnamon zeylanicum L.) essential oil (CEO), nanoemulsion of the essential oil was prepared and its physical and chemical properties were determined. The particle size of nanoemulsion was determined to be
115.33 ± 3.97 nm. Emulsification and nanoemulsion of the essential oil along with thiabendazole as an antifungal agent at various concentrations of active ingredient were studied for control of Rhizopus stolonifera and Botrytis cinerea fungi, strawberry fruit decay. Results in solid Potato Dextrose Agar (PDA) medium indicated that emulsion and nanoemulsion of CEO have a significant difference in antifungal activity against B. cinerea and R. stolonifera. The minimum inhibitory concentration was 500 and 1,000 μl fungi per liter of culture medium. According to the results of the research, essential oil nanoemulsion had a significant effect on the reduction of a fungal cartilage of strawberry fruit. In general, nano-emulsions of the essential oil showed more antifungal activity than essential oil. There was no significant difference in decay control between thiabendazole and CEO. The nano-emulsion of cinna- mon oil at a concentration of 0.2% proved significant effect in reducing fruit decay and showed the lowest fruit infec- tion (5.43%). Consequently, nano-emulsion of essential oil is recommended for the production of natural fungicides.
Caracterización fenólica y capacidad antioxidante de extractos alcohólicos de hojas crudas y hervidas de Cnidoscolus aconitifolius (Euphorbiaceae)
Authors: Godínez-Santillán Rosa Iris , Chávez-Servín Jorge Luis , García-Gasca Teresa , Guzmán-Maldonado Salvador Horacio 
Published: June 2019

Antecedentes y Objetivos: Cnidoscolus aconitifolius es una especie arbustiva que se utiliza como planta ornamental, medicinal y alimentaria en varias partes del mundo, conocida en México como chaya. Existe poca investigación científica publicada sobre su composición fenólica y su capacidad antioxidante. El objetivo de este estudio es determinar la caracterización fenólica y la capacidad antioxidante de extractos hidroalcohólicos (metanol-agua y etanol-agua) en diferentes proporciones de hojas de C. aconitifolius crudas y hervidas.Métodos: Se realizó un cultivo de C. aconitifolius en el campus de la Universidad de Querétaro (México; 2014) utilizando estacas leñosas. Durante el verano de 2016 se realizaron muestreos de hojas de todas las plantas cultivadas. El contenido de compuestos fenólicos totales (CFT), el contenido total de flavonoides (CTF) y la capacidad antioxidante se determinaron en extractos de metanol-agua (50:50 y 80:20) y de etanol-agua (50:50 y 80:20) provenientes de hojas crudas y hervidas. Además, se identificaron y cuantificaron compuestos fenólicos de cada uno de los extractos mediante el método RP-HPLC-DAD.Resultados clave: Se identificaron y cuantificaron 11 compuestos fenólicos en la especie C. aconitifolius: ácido gálico, ácido vanílico, vainillina, ácido clorogénico, ácido cafeico, ácido ferúlico, ácido rosmarínico, ácido p-cumárico, resveratrol, luteolina y apigenina. En general, las hojas hervidas mostraron una mayor capacidad antioxidante que las hojas crudas. Se observaron diferencias en el contenido de compuestos fenólicos debido al tipo de solvente y mezcla utilizada, siendo el extracto etanólico 80:20 proveniente de las hojas hervidas de chaya el que mostró mayor contenido de polifenoles.Conclusiones: Hervir las hojas incrementó el contenido de compuestos fenólicos en los extractos analizados. Los resultados de este estudio indican que las hojas de chaya son una fuente rica de antioxidantes naturales.
Treinamento da musculatura respiratória em distonia responsiva a levodopa
Authors: Pesente Tainá , Bonfanti Gabriela Tais Ferreira , Camera Fernanda Dal’Maso , Morsch Ana Lucia Bernardo de Carvalho , Petry Ana Laura Nicoletti Carvalho 
Published: June 2019

Introdução: A distonia pode ser definida como um distúrbio hipercinético do movimento, constituído por contrações involuntárias que provocam perturbações da postura e alterações na execução dos movimentos. O tônus pode variar em hipotonia, normotonia e hipertonia, e estas variações podem surgir ou piorar em função da intensidade do movimento e do estado emocional do paciente. Verificou-se que indivíduos com distonia responsiva a levodopa podem sofrer uma diminuição de sua capacidade muscular, ficando assim predispostos às doenças pulmonares. Objetivos: Avaliar os efeitos de um protocolo de treinamento muscular expiratório (TME) na força muscular respiratória em diferentes posturas emum paciente com distonia responsiva a levodopa. Materiais e métodos: A amostra foi composta por um paciente com distonia responsiva a levodopa, 18 anos, sexo feminino. Foram realizadas avaliações de força muscular respiratória e pico de fluxo expiratório (PFE) pré e pós-treinamento muscular. O TME foi realizado com resistor expiratório de carga linear durante um mês, três vezes por semana com carga de 25% da pressão expiratória máxima (PEmáx) inicial por 15 minutos. Resultados: Obteve-se um aumento no valor das pressões respiratórias máximas no pós-tratamento em relação ao momento pré-intervenção. Sendo que na posição sentada a paciente apresentou um aumento de 50% na pressão inspiratória máxima (PImáx) e 62,16% na PEmáx. Já na posição supina o aumento foi de 81,25% na PImáx e 36,36% na PEmáx. Todos os valores de PFE encontrados no pré e pós-treinamento se encontram abaixo dos valores preditos para paciente, embora, tenha-se observado elevação de 10 L/min no pós-tratamento em relação ao pré-tratamento, valores discretamente elevados (+ 6,06%) foram encontrados na posição sentada. Conclusão: Conclui-se que cargas baixas de treinamento muscular são eficazes no aumento das forças musculares inspiratórias e expiratórias nas posições estudadas, no entanto, parecem insuficientes para alteração do PFE.
Tratamento fisioterapêutico de pubalgia em atleta de futsal profissional: estudo de caso
Authors: Silva Júlia Raqueli da , Frank Ketryn Danieli , Silva Márcia Regina da 
Published: June 2019

Introdução: A pubalgia é descrita, do ponto de vista fisiopatológico, como um processo inflamatório que acomete a sínfise púbica, produzindo alterações condrais e ósseas nesta articulação. Calcula-se que 5% de todas as lesões no futebol ocorrem na região do púbis e virilha. Objetivos: O objetivo do presente estudo é identificar quais os resultados da fisioterapia no atleta de futsal com história de lesão em adutor e diagnóstico de pubalgia. Materiais e métodos: O estudo de caso foi realizado com um jogador de futsal profissional com 17 anos, atuante na posição de pivô. O atleta foi avaliado e as intervenções foram realizadas nas dependências do laboratório de Fisioterapia da Universidade Comunitária da Região de Chapecó (Unochapecó). As sessões foram baseadas em exercícios de alongamento e fortalecimento muscular, realizadas uma vez por semana, não consecutivas. Resultados: Na reavaliação o jogador apresentou ganho de amplitude de movimento em adução e abdução do quadril, aumento da flexibilidade muscular em adutores, abdutores, isquiotibiais e quadríceps e diminuição do quadro álgico em região pélvica e adutor de membro inferior esquerdo. Conclusão: Conclui-se que o tratamento realizado no atleta foi capaz de aumentar a flexibilidade, amplitude de movimento e diminuir a dor na região do púbis.
Método Pediasuit™ no tratamento da paralisia cerebral: relato de casos
Authors: Budtinger Lillian Franciele , Müller Alessandra Bombarda 
Published: June 2019

Introdução: A Paralisia Cerebral (PC), resultado de um dano cerebral ocorrido durante a formação do encéfalo, pode ocasionar variadas manifestações. Atualmente, o Método Pediasuit™, que consiste na utilização de uma roupa especial constituída por touca, colete, calção, joelheiras e calçados próprios interligados a bandas elásticas e ganchos, no intuito de manter o alinhamento corporal adequado, é considerado um tratamento promissor para os distúrbios sensoriais e motores presentes na PC. Objetivo: Verificar os efeitos do Método Pediasuit™, observando a função motora grossa e o desempenho funcional de duas crianças com PC. Método: Estudo do tipo relato de caso, onde a função motora e o desempenho funcional de dois meninos, com diagnóstico clínico de PC do tipo diparesia e tetraparesia espástica, foram avaliados antes e depois de quatro semanas de tratamento intensivo com o método proposto, com duração de 3h15min/dia, totalizando 65h. Resultados: O Método Pediasuit™ favoreceu a função motora grossa e o desempenho funcional das crianças submetidas ao tratamento intensivo. Foram constatadas mudanças expressivas no desempenho motor dos participantes: incremento motor de 12,42% e 4,79%. Entretanto, ambos permaneceram no mesmo nível de classificação funcional. Considerações finais: Os resultados aqui apresentados apontam melhor desempenho motor após o tratamento dos sujeitos avaliados. Entretanto, não é possível afirmar que este método promove melhoria significativa, porque seria necessário o acompanhamento por um período mais prolongado.
Gelenek Kültürü, Medya Müzeciliği ve YouTube
Authors: GÜLÜM Erol 
Published: June 2019

Siberuzamın maddi kültürel unsurların temsili ve sunumu hususunda da yeni fırsatlar sunduğunu fark eden araştırmacılar bir kültürel pratik olarak müzeciliği, bir kültürel kurum olarak müzeyi ve bir kültürel etkinlik biçimi olarak müzesel iletişimi, ‘medya müzeciliği’ kavramı ekseninde yeniden teorize etmiştir. Bahsi geçen teori özelinde	yürütülen çalışmalarda ise genelde fiziksel mekânın imgelemsel eşleniği olarak algılanan siberuzamın müzelik içeriklere içkin kültürel bilgiyi tanılayıcı, koruyucu ve sergileyici bir yapıya sahip olup olmadığı ve bu kapsamda müzesel iletişimi mümkün kılacak bağlamlar sunup sunmadığı tartışılmıştır. Günümüzde de sürmekte olan bu türden tartışmalara medya müzeciliğinin gelenek kültürü içeriklerinin temsili ve sunumuna imkan tanıyan çeşitli sosyal medya platformlarının müzesel bir alan olarak deneyimlenmesini de açıklayabilecek kadar geniş bir kavramsal kaplama sahip	olduğu iddiasıyla müdahil olunabilir. İşte bu tez ifadesi kapsamında çalışmada her an ve her noktadan erişim, hız, çeşitlilik, bilgi odaklılık, etkileşimsellik ve katılımcılık vadeden yeni medya teknolojileri ve kültürünün başat sembollerinden biri olan YouTube’un genellikle sözlü ve bedensel folklorik dışavurumlarda somutlaşan, geleneksel bilgi ve deneyim belleğinin taşıyıcısı olan gelenek kültürü içeriklerinin arşivlenmesi, temsili ve sunumuna olanak tanıyan bir müzesel alan olarak nasıl deneyimlendiği disiplinlerarası bir perspektif özelinde ve tekno-sosyo-kültürel boyutlarıyla tartışılmaktadır.