bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening of lung cancer biomarker-proteins with a multiplex electrochemical sensor system based on aptamers
Authors: Glazyrin Yu. E. , Shabalina A. V. , Ryginskaya K. A. , Zamay S. S. , Kolovski V. A. , Svetlichnyi V. A. , Lapin I. N. , Zamay G. S. , Kolovskaya O. S. , Zamay T. N. , Pac Yu. S. , Kichkailo A. S. 
Published: October 2018

The aimof this work is the development and demonstration of the method of simultaneous detection of several biomarkers of lung cancer in the blood plasma of patients using a multiplex electrochemical testing system based on DNA aptamers. DNA aptamers are a new class of synthetic affinity probes obtained by in vitro or in vivo selection procedure by the systematic evolution of ligands by exponential enrichment (SELEX).Materials and methods.A set of aptamers obtained previously by selection for postoperative lung cancer tissue was used to create a multiplex electrochemical biochip. Identification of aptamer target proteins was performed using a modified affinity enrichment method (AptaBID). Molecular targets for the used set of aptamers to lung cancer were defined as vimentin, defensin, a light chain of myosin, tubulin alpha 1-B, neutrophil elastase and A1 elongation factor 1.Measurements of the presence of these biomarker proteins in blood plasma were carried out using electrochemical detection. The difference between peak heights before and after plasma deposition on the electrodes modified by aptamers was considered as a response of the system to the presence of protein onco-markers in blood plasma. Blood plasma of healthy volunteers was used as control.Results. Research showed that in the blood plasma of all the patients with lung cancer the content of biomarker proteins that bind to aptamers on electrode surfaces was increased. The increased content of these proteins in the blood plasma of patients suggests the presence of invasiveness and metastasis of tumors and their chemo-resistance.
Prognosis of cardiovascular events after implantation of a cardioverterdefibrillator in patients with chronic heart failure: the value of increasing concentration of endothelin-1 and soluble forms of ST2 protein in blood plasma
Authors: Teplyakov A. T. , Tarasov N. I. , Isakov L. K. , Grakova E. V. , Sinkova M. N. , Kopieva K. V. , Garmaeva О. B. , Ogurkova O. N. , Kalyuzhin V. V. , Kalyuzhina E. V. 
Published: October 2018

Aim.The purpose of this study was to examine the association of increasing concentrations of endothelin-1 and soluble forms of ST2 protein (sST2) in the blood plasma of patients with chronic heart failure (CHF) who underwent implantation of a cardioverter-defibrillator, with the development of cardiovascular events (death from cardiovascular diseases, decompensated CHF, acute coronary syndrome or acute ischemic stroke) to determine whether these biomarkers can be used as predictors of an unfavorable course of the disease.Material and methods. A 6-month cohort study included 40 patients with CHF who received an ICD 4-12 weeks before their inclusion in the study. At the beginning of the study the concentrations of endothelin-1 and sST2 in plasma by ELISA were analyzed, along with conventional diagnostic techniques typical of a specialized cardiology clinic.Results.Phenotype with a favorable course of the disease was identified in 21 patients (group 1), and with a unfavorable course was seen in 19 (group 2). The concentration of endothelin-1 and sST2 in the blood plasma of the 2nd group patients was higher than in patients of the 1st group. The link of cardiovascular events with high levels of soluble ST2 protein and endothelin-1 in blood plasma has been identified. Variation of the decision rule threshold on the ROC-curve has allowed sST2 concentration of 34.93 ng/ml to be determined as a cutoff point. Accuracy of two-class classification (determination of the posterior probability of cardiovascular events) after identifying such cut-off point was characterized by 93% sensitivity and 72% specificity (area under ROC-curve – 0.87). If the value of the plasma concentrations of sST2 more 34.93 ng/ml the relative risk of cardiovascular events was 4.4 (95% CI 1.7–11.1). The use of endothelin-1 surplus in plasma (cut-off point – 0.34 fmol/ml) in predicting cardiovascular events was lower: the sensitivity – 88%, specificity – 68% (area under ROC-curve – 0.74). When the concentration of endothelin-1 in blood plasma was more than 0.34 fmol/ml the relative risk of cardiovascular events was 2.7 (95% CI 1.2–6.3).Conclusion.The increase in the sST2 and endothelin-1 concentration in blood plasma may be considered as a predictor of cardiovascular events in CHF patients after implantation of cardioverter-defibrillator.
The role of gastrointestinal pathology in the development and severity of oral lichen planus
Authors: Titarenko M. A. , Stolyarova V. A. , Sysolyatin P. G. , Baydik O. D. 
Published: October 2018

The aim is to determine the most significant predictors of the oral lichen planus in patients with gastrointestinal pathology.Materials and methods. The study was performed of 40 patients aged 41 to 86 years with oral lichen planus. Degree of mucosal damage by oral lichen planus was evaluated. The concomitant pathology of the gastrointestinal tract was established. A discriminant analysis was carried out.Results. The most significant predictors for a diagnosis of oral lichen planus in patients with gastrointestinal pathology are revealed. The sensitivity of the method was 100.0%, the specificity was 100.0%, and the errorfreeness was 100.0%.The conclusion. Discriminant analysis allows us to conduct a diagnosis of the oral lichen planus in patients with gastrointestinal pathology. This model is available in clinical practice.
Hemocompatibility of magnetic magnethite nanoparticles and magnetite-silica composites in vitro
Authors: Toropova Y. G. , Pechnikova N. A. , Zelinskaya I. A. , Korolev D. V. , Gareev K. G. , Markitantova А. S. , Bogushevskaya V. D. , Povolotskaya A. V. , Manshina A. A. 
Published: October 2018

The goal of the present research is to study the hemocompatibility of magnetic nanoparticles (MNPs) in model systems in vitro.Materials and methods. Magnetite nanoparticles and magnetite colloidal solutions were used in 0.9% NaCl in concentrations 0.2, 2.0 and 20.0 mg/ml. The study was performed with heparinized human whole blood, 1 ml of which was mixed with 1 of ml nanoparticles/physiological solution. Measurements were made directly after mixing, and then 1, 2.5 and 5 hours later. The amount of reactive oxygen species (ROS) was measured with luminol-dependent chemiluminiscence (CL). An erythrocyte aggregation index was calculated. For the assessment of hemolytic properties, a hemolysis coefficient was calculated based on optical density of the plasma. The nanoparticless surface protein layer investigation was performed with IR-Fourier spectroscopy.Results. Nanoparticles decline CL in timeand concentration-dependent manner. Erythrocyte aggregation stability grows, but concentration and/or application time increment leads to significant hemolysis. IR-Fourier spectroscopy data shows albumin as main component of protein crown, whose conformation changes in time.Given data proves safety of studied MNPs in relation to examined parameters in low (0.2 and 2.0 mg/ml) concentrations up to 2.5 hours interaction. This allows us to treat these MNPs as a promising agents for further use in medical practice after completing examinations related to other homeostasis indicators.
Features of immunoregulation in patients with pulmonary tuberculosis with blood eosinophilia
Authors: Urazova O. I. , Churina E. G. , Kolobovnikova Yu. V. , Novitskiy V. V. , Karaulov A. V. , Nikulina E. L. , Poletika V. S. 
Published: October 2018

The aimof the investigation was to determine the characteristics of the immune response regulation for pulmonary tuberculosis (TB) and to analyze the role of regulatory T cells in the immunopathogenesis of TB with eosinophilia in the blood, depending on the clinical form of the disease and sensitivity of Micobacterium tuberculosis to anti-TB drugs.Materials and methods.157 patients who were initially diagnosed with infiltrative and disseminated TB were examined. The material of the study was venous blood and culture of mononuclear leukocytes isolated from venous blood. The content of interleukin (IL) 4, IL-10 and transforming factor beta (TGFβ) in culture suspensions of mononuclear leukocytes in vitro and IL-5 in the blood was determined by enzyme-linked immunosorbent assay (ELISA) test. The expression of surface molecules CD4, CD20, CD25 and intracellular transcription factor Foxp3 by lymphocytes of the blood was evaluated by flow cytometry. The obtained results were analyzed by statistical methods.Results.It is shown that excessive generation of regulatory T cells in patients with TB is associated with eosinophilia of the blood and imbalance of immune response regulation mechanisms. In TB with eosinophilia, an increase in the number of Foxp3-positive regulatory T cells in the blood is combined with in vitro hypersecretion of anti-inflammatory cytokines TGFβ, IL-10, IL-4 and an increase in the content of CD20+ B lymphocytes and IL-5 in the blood. These changes are most pronounced in the disseminated form of TB in combination with drug resistance.Conclusion.Characteristics of immunoregulation at TB with blood eosinophilia are associated with activation of immunosuppression mechanisms and polarization of immune response towards Th2-dependent pathway.
Adaptive reactions of rats after light desynchronosis and physical overwork
Authors: Gostyukhina A. A. , Zamoshchina T. A. , Zaitsev K. V. , Gutor S. S. , Zhukova O. B. , Svetlik M. V. , Abdulkina N. G. , Zaitsev A. A. 
Published: October 2018

It is known that desynchronosis and physical stress to the point of fatigue are powerful stressors for the body. Studies indicate a depleting effect of the combined stress factors on the adaptive reserves of the organism, especially when the joint exposure is prolonged. However, the adaptive value of the training process in various activities is also well known. In this regard, it seems important to study these two aspects of adaptation when combined effects of light desynchronosis and physical overwork on the body.Рurposeof this study was to study the features of adaptive reactions of rats under conditions of light desynchronosis and physical overwork.Materials and methods. An experimental study was performed on 60 mature male rats of the Wistar breed. For the induction of experimental desynchronosis, the animals of the experimental groups were kept for 10 days in artificial bright illumination (150 LX) or full darkening (2-3 LX). The model of physical overfatigue used the method of forced swimming of rats to complete fatigue in their own modification. At the end of the swim test, all the animals were withdrawn from the experiment and underwent one-step-long decapitation under CO2 with anesthesia. In decapitated animals, to collect serum, the blood was collected in a clean, dry vial. In the blood serum of rats, the biochemical standard method was used to determine the level of lactate. Using the ELISA method and the reagent kit “IBL” (Germany), the concentrations of corticosterone and serotonin were determined. The histological analysis of the stress-realizing organs was carried out according to a standard procedure using light microscopy using the Axioskop 40 microscope from CarlZeiss (Germany).Results.It was found that physical fatigue in rats after daily swimming activities (5 days) was accompanied by a decrease in the level of corticosterone in the blood serum and destructive changes in the adrenal and femoral muscles in comparison with intact animals. In comparison with the intact group, dark deprivation and physical activity did not alter the content of corticosterone, serotonin, and lactate in the blood and caused minor destructive processes in the adrenal glands. Light deprivation and fatigue lowered the level of corticosterone in the blood, caused destructive changes in the adrenal glands and muscles, and increased serotonin levels in serum, but did not change the level of lactate.Conclusions.The features of adaptive reactions of rats under conditions of light desynchronosis and physical overfatigue are determined by the nature of deprivation or the direction of the phase shift (deprivation of light or darkness). Dark deprivation followed by physical overfatigue causes the development of the resistance phase of the general adaptation syndrome, and light deprivation followed by physical overwork is the phase of exhaustion.
The problem of assessing the adaptive capacity of the human operator
Authors: Popechitelev E. P. , Pekker Ya. S. , Novikova T. V. 
Published: October 2018

Decomposition of professional thinking of the human operator in process control, requiring intense attention for a long time, was performed. The problem of assessing the ability of the operator to adapt to mental stress was formulated. The authors proposed a model for calculation of the indicators of the functional state of the body during the transition from normal to hard work. It is based on a dynamic model of the system, which is not in a stationary state under external forces, but will be changing until the rate of entropy production within the system reaches the minimum in these conditions. It is shown that this model can be used to solve the problem.
The results of surgical treatment of patients with large and complex incisional hernias of the anterior abdominal wall in a multidisciplinary hospital
Authors: Degovtsov E. N. , Kolyadko P. V. , Kolyadko V. P. , Satinov A. V. 
Published: October 2018

Objectives. To evaluate the immediate results of surgical treatment of patients with large incisional hernia of the anterior abdominal wall with the use of the mesh implants.Materials and methods. The data were retrospectively collected and analyzed. The study included patients with a large and / or complex incisional hernia. There were 108 patients who underwent incisional hernia repair with mesh reinforcement in the period from 2012 to 2016. In all cases the repairs were made with mesh implants. The average age of patients was (56.4 ± 10.4) years. The body mass index (BMI) was on average (32.6 ± 6.24) kg/m2. The medial localization of the hernia was 102 (94.44%). The width of the hernia defect averaged (12.2 ± 3.7) cm. Implants were placement in onlay positions – 19 (17.6%), sublay – 49 (45.37%), IPOM (Intraperitoneal onlay mesh) – 30 (27.77%) patients, the component separation technique (CST) with mesh reinforcement was used in 10 (9.25%) patients. Active aspiration drainage was performed in 72 (66.66%) patients.Results. The average time for draining the postoperative wound was (5 ± 2.2) days. The number of wound complications was 23 (21.3%), the number of seromas of the postoperative wound prevailed was 16 (14.8%) patients, of which 2 (1.85%) were chronic abdominal wall seromas, hematoma occurred in 2 patients (1.85%), the number of prolonged serous exudation was 7 (6.5%), necrosis of the wound edges occurred in 4 (3.7%) patients. There was no mortality. Reliably more often wound complications occurred in patients with large hernia defects (p = 0.006), and also with an increase in the duration of surgical intervention (p = 0.01). The hospital-stay in patients with complications was significantly greater (p < 0.001), the need for analgesics also increased (p < 0.001).Conclusion. Prevention of wound complications after large and complex incisional hernia repair with mesh reinforcement is an important direction in improving the results of surgical treatment in this category of patients.
Use of ultrasonic elastography in the differential diagnosis of pancreatic cystic lesions
Authors: Dibina T. V. , Drozdov E. S. , Koshel A. P. , Latypov V. R. 
Published: October 2018

Aim. Evaluate the effectiveness of ultrasonic elastography in the differential diagnosis of pancreatic cystic lesions.Materials and methods. Seventy patients with pancreatic cystic lesions were examined. Structure of clinical forms is the following: cystadenoma – 30 (serous cystadenoma – 23, mucinous cystadenoma – 2, mucinous cystadenocarcinoma – 5), pseudocysts – 40. Diagnosis of pancreatic cystic lesions was conducted with the help of transabdominal ultrasound examination (with 3D-reconstruction) and ultrasonic elastography.Results. The parameters of ultrasonic elastography for various types of pancreatic cystic lesions were determined. Sensitivity, specificity, positive predictive value, negative predictive value, the overall accuracy of the technique accounted for 97, 75, 74, 97 and 84% respectively. The superiority of the elastography technique over the standard transabdominal ultrasound in differential diagnosis of cystic pancreatic formations was illustrated.Conclusions. Ultrasonic elastography is an effective, non-invasive method of differential diagnosis of pancreatic cystic lesions and can be widely used in clinical practice.
The effect of canceroembryonic antigen on cytokine production by immunocompetent blood cells in patients with breast cancer
Authors: Autenshlus A. I. , Kunts T. A. , Karpukhina K. V. , Mikhailova E. S. , Varaksin N. A. , Marinkin I. O. 
Published: October 2018

Aim. Investigation of the influence of canceroembryonic antigen (CEA) on the immune cells in patients with breast tumors.Materials and methods. Peripheral blood of 67 patients with invasive ductal carcinoma (n = 44), fibroadenoma (n = 12) and breast pre-cancer (n = 11). In the supernatants of the blood cells cytokine production induced by CEA was determined by ELISA.Results.It was found that CEA stimulated production of IL-6, IL-8, IL-10, IL-1β, IL-1Ra, TNFα, G-CSF, GM-CSF and the protein MCP-1 by blood immune cells in patients with fibroadenoma compared with groups of patients with invasive ductal carcinoma and precancerous lesions. Stimulation index of CEA on cytokine production by immunocompetent cells in patients in these groups was lower than in groups of patients with fibroadenoma due to initially high levels of spontaneous cytokine production.Results. In patients with fibroadenoma CEA was found to stimulate the production of IL-6, IL-8, IL-10, IL-1β, IL-1Ra, TNFa, G-CSF, GM-CSF and MCP-1 compared with groups of patients with invasive ductal carcinoma and precancerous conditions. Indexes of the influence of CEA on cytokine production by immunocompetent cells in these groups was lower than in the fibroadenoma due to initially high spontaneous production of cytokines.Conclusion. Evident spontaneous cytokine-producing function of immunocompetent blood cells was revealed in patients with invasive ductal carcinoma and precancerous conditions compared to patients with fibroadenoma. The differences between the indices of CEA influence on cytokine production in patients with malignant, benign and precancerous conditions can serve as a basis for the development of methods of differential diagnosis of breast tumors.
Molecular features of head and neck squamous cell carcinoma
Authors: Kakurina G. V. , Kolegova E. S. , Cheremisina О. V. , Choinzonov Е. L. 
Published: October 2018

Relevance.To identify new markers of early diagnosis and prognosis of head and neck squamous cell carcinoma (HNSCC) it is necessary to study the molecular features of this disease.Purpose.The aim of the study was to analyze blood serum protein spectrum in patients with HNSCC and in healthy volunteers using the methods of mass spectrometry and to evaluate the selected serum protein markers as candidates for early detection of HNSCC.Materials and Methods: The blood serum of HNSCC patients before therapy with metastases, without metastases and healthy volunteers was studied by proteomic methods. Validation of the results of proteomic analysis was carried out by ELISA in serum of 52 patients with HNSCC (T1-4N0-3M0), 10 patients with chronic hyperplastic laryngitis, dysplasia DII-DIII and 10 healthy volunteers. The statistical analysis was carried out using Statistica 6.0. Software package.Results.Blood serum proteome of HNSCC patients with metastases, without metastases and healthy volunteers are different and contain proteins of different classes. Adenylyl cyclase-associated protein 1 (CAP1) and protein phosphatase 1B (PPM1B) were selected to validate the obtained results. It was shown that the serum level of CAP1 and PPM1B differed in control and dysplasia groups and dysplasia and cancer groups (p ≤ 0,05). In patients with HNSCC (T1N0M0) the serum CAP1 and PPM1B levels were higher than in patients with dysplasia and healthy individuals (p ≤ 0,05). It was noted the positive correlation of the CAP1 level in the serum with the presence of metastases and the PPM1B level.Conclusion.Candidates for serum markers of HNSCC prognosis were identified. The difference in serum levels of CAP1 and PPM1B depending on the prevalence of primary tumors and the difference in serum level of CAP1 depending on the presence of regional metastases was shown. Determination of CAP1 level in the serum can be useful for early diagnosis and prognosis of HNSCC.
Prevention of pathological syndromes and post-morbidity rehabilitation of patients with heavy surgical infection on the basis of the complications development prediction index
Authors: Kemerov S. V. , Vorobjev V. M. , Karpova M. R. , Solov᾿ev M. M. , Dambaev G. Tz. 
Published: October 2018

Purpose of the study: working out an easy-to-use complication development index (CDI), designing a program for post morbidity rehabilitation of patients with surgical infection to improve the results of its treatment and reduce lethality.Materials and methods. A retrospective results analysis of treatment of 320 patients for purulent peritonitis and abdominal sepsis was carried out, including 268 patients (the comparison group) and 52 (the core group) with whom the complications development index was calculated. While studying this group of patients, the same protocol was used to prevent and treat complications on the basis of a complex individual program. In the comparison group, the patients were divided according to the severity of their condition in compliance with generally accepted clinical criteria: medium-heavy (98), severe (86) and extremely severe (84 patients, respectively).We have studied general clinical and biochemical indices, as well as the results of instrumental studies of the function of vital organs and body systems. The following indices were calculated: the prothrombin index; the integral index of thromboelastography (IIT), J = [R (min) × K (min)] : mA (mm), where R is the clotting time, K is the clot formation time, mA is the clot density); the general condition severity APACHE II (Acute physiology and chronic health evaluation); the degree of organ dysfunction SOFA (Sepsis organ failure assessment); leukocyte index of intoxication (LII). The PIRO concept (predisposition, infection, response, organ dysfunction) was used for clinical interpretation of the received research results.Results. The prediction index of complications of surgical infection was proposed. It is based on the results of clinical analysis the results of patient treatment and calculated by using the severity of the underlying pathological process, as well as the number of concomitant diseases, pathological syndromes and aggravating factors. As a result of the complication development prediction index use along with the implementation of the complex program for prevention and treatment of complications, the number went down from 50% to 90% in different groups under observation.