bluuebunny/crossref_metadata_2025_split huggingface dataset:
The gene regulatory network in different brain regions of neuropathic pain mouse models
Authors: Li Xia , Xu Long-Sheng , Xu Yu-Fen , Yang Qi , Fang Zhi-Xian , Lv Xiao-Dong , Yao Ming , Chen Wen-Yu 
Published: July 2019

Abstract
Neuropathic pain is the direct result caused by lesions or somatosensory nervous system diseases that are associated with emotional regulation. The incidence of neuropathic pain in the general population is 7-10% and the mechanisms of neuropathic pain are largely unknown. It is often related to structural and functional abnormalities in multiple brain regions. The forebrain, including nucleus accumbens (NAc), medial prefrontal cortex (mPFC) and periaqueductal gray (PAG) have been shown to correspond with the regulation of neuropathic pain. To investigate the molecular mechanism of neuropathic pain across different brain regions, we identified the differentially expressed genes between the spared nerve injury model (SNI) mice of neuropathic pain and the control Sham mice in NAc, mPFC and PAG and mapped these genes onto comprehensive functional association network. With Random Walk with Restart (RWR) analysis, we identified more novel neuropathic pain genes in NAc, mPFC and PAG, such as Asic3, Cd200r1 and MT2, beside well known Capn11 and CYP2E1. What’s more, we discovered their interactions or cross talks. Our results provided novel insights of neuropathic pain and provided therapeutic targets for treating neuropathic pain.
Phytochemical and comparative transcriptome analysis reveals different regulatory mechanisms in terpenoids pathways between Matricaria recutita L. and Chamaemelum nobile L
Authors: Tai Yuling , Hou Xiaojuan , Liu Chun , Sun Jiameng , Guo Chunxiao , Su Ling , Jiang Wei , Ling Chengcheng , Wang Chengxiang , Wang Huanhuan , Pan Guifang , Si Xiongyuan , Yuan Yi 
Published: July 2019

Abstract
Background

Matricaria recutita (German chamomile) and Chamaemelum nobile (Roman chamomile) belong to the botanical family Asteraceae. These two herbs are not only morphologically distinguishable, but their secondary metabolites – especially the essential oils in flowers are also different, especially the terpenoids. The aim of this project was to preliminarily reveal differential regulatory mechanisms in terpenoids biosynthetic pathway between German and Roman chamomile by performing comparative transcriptomic and metabolomics analyses.

Results

We determined the content of essential oil in disk florets and ray florets in these two chamomiles, and found that the terpenoids content of German chamomile is greater than that of Roman chamomile. In addition, comparisons between German and Roman chamomile were studied by RNA-Seq, which showed that 54% of genes shared >75% sequence identity between the two species. In particular, more highly expressed DEGs (differentially expressed genes) and TFs (transcription factors), different regulation of CYPs (cytochrome P450 enzymes) and rapid evolution of the downstream enzymes in terpenoids biosynthetic pathways in German chamomile, and maybe the reasons result to differences in the types and levels of terpenoids compounds in these two chamomiles. In addition, the phylogenetic tree of single copay genes showed that German chamomile and Roman chamomile had a high identity to C. nankingense.

Conclusion

This work provided first insights into the terpenoids biosynthesis of chamomiles, and the candidate unigenes related to terpenoids biosynthesis can serve as important information for molecular breeding approaches to modulate the essential oil composition.
Urinary proteome of dogs with kidney injury during babesiosis
Authors: Winiarczyk Dagmara , Michalak Katarzyna , Adaszek Łukasz , Winiarczyk Mateusz , Winiarczyk Stanisław 
Published: July 2019

Abstract
This study aimed to identify proteins in the urine of dogs with renal dysfunction during the natural course of babesiosis (n=10) and to compare them with proteins in a control group (n=10) to reveal any potential biomarkers of renal damage. Pooled urine samples from both groups were separated by 2D (two-dimensional) electrophoresis, followed by protein identification using MALDI-TOF (matrix-assisted laser desorption ionization time of flight) mass spectrometry. In total, 176 proteins were identified in the urine samples from healthy dogs, and 403 proteins were identified in the urine samples from dogs with babesiosis. Of the 176 proteins, 146 were assigned exclusively to healthy dogs, and 373 of the 403 proteins were assigned exclusively to dogs with babesiosis; 30 proteins were common to both groups. Characteristic analysis of the 373 proteins found in dogs with babesiosis led to the isolation of 8 proteins associated with 10 metabolic pathways involved in immune and inflammatory responses. Furthermore, it was hypothesized that epithelial-mesenchymal transition might play an important role in the mechanisms underlying pathological changes in renal tissue during babesiosis, as indicated by a causal relationship network built by combining 5 of the 10 selected metabolic pathways and 4 of the 8 proteins associated with these pathways; this network included cadherins, gonadotropin releasing hormone receptors, inflammatory responses mediated by chemokine and cytokine signalling pathways, integrins, interleukins and TGF-β (transforming growth factor β) pathways. These pathways were linked by interleukin-13, bone morphogenetic protein 7, α2(1) collagen, and FER tyrosine kinase, which are potential biomarkers of damage during babesiosis in dogs that might indicate early renal injury.
The use of TAP for the primary diagnosis of renal cell carcinoma
Authors: Jiang Linjun , Liu Yili , Qu Hongchen , Dong Fengming 
Published: July 2019

Abstract
Objective

To study the value of TAP expression in peripheral blood for early diagnosis of renal cell carcinoma.

Materials and Methods

TAP expression was analyzed in the peripheral blood of 45 patients with renal cell carcinoma, 32 patients with benign renal lesions and 42 healthy patients. An analysis of the relationship between TAP expression and the clinicopathological features in renal cell carcinoma was performed; the ROC curve was used to evaluate the diagnostic efficacy of TAP detection for renal cell carcinoma diagnosis.

Results

TAP levels were significantly higher in RCC patients than in patients with nonneoplastic diseases. We also found that the rate of positive TAP expression in the peripheral blood of the renal cell carcinoma group showed no significant difference when evaluated by age, sex, histological grade, tumor stage, tumor size or tumor location (P > 0.05). The area under the ROC curve was 0.92 (95% CI: 0.82-0.95), and the sensitivity and specificity in the diagnosis of RCC was 73% and 91%, respectively.

Conclusions

The expression of TAP in peripheral blood of patients with renal cell carcinoma was significantly higher than that in nonneoplastic patients, which may provide an effective means of primary diagnosis for renal cell carcinoma
Surgical Configurations of the Pectoralis Major flap for Reconstruction of Sternoclavicular Defects: A Systematic Review and New Classification of Described Techniques
Authors: Opoku-Agyeman Jude , Matera David , Simone Jamee 
Published: July 2019

Abstract
Objectives

The pectoralis major flap has been considered the workhorse flap for chest and sternoclavicular defect reconstruction. There have been many configurations of the pectoralis major flap reported in the literature for use in reconstruction sternoclavicular defects either involving bone, soft tissue elements, or both. This study reviews the different configurations of the pectoralis major flap for sternoclavicular defect reconstruction and provides the first ever classification for these techniques.

Methods

EMBASE, Cochrane library, Ovid medicine and PubMed databases were searched from its inception to November of 2018. We included all studies describing surgical management of sternoclavicular defects. The studies were reviewed, and the different configurations of the pectoralis major flap used for sternoclavicular defect reconstruction were cataloged. We then proposed a new classification system for these procedures.

Results

The study included 5 articles published in the English language that provided a descriptive procedure for the use of pectoralis major flap in the reconstruction of sternoclavicular defects. The procedures were classified into three broad categories. In Type 1, the whole pectoris muscle is used. In Type 2, the pectoralis muscle is split and either advanced medially (type 2a) or rotated (type 2b) to fill the defect. In type 3, the clavicular portion of the pectoralis is islandized on a pedicle, either the TAA (type 3a) or the deltoid branch of the TAA (type 3b).

Conclusion

There are multiple configurations of the pectoralis flap reported in the English language literature for the reconstruction of sternoclavicular defects. Our classification system will help facilitate communication when describing the different configurations of the pectoralis major flap for reconstruction of sternoclavicular joint defects.
Parvovirus B19 infection and renal injury: report of 4 cases and analysis of immunization and viremia in an adult cohort of 100 patients undergoing a renal biopsy.
Authors: Kauffmann Maëlis , Bobot Mickaël , Daniel Laurent , Knefati Yannick , Moranne Olivier , Burtey Stéphane , Zandotti Christine , Jourde-Chiche Noemie 
Published: July 2019

Abstract
BACKGROUND: The seroprevalence of human Parvovirus B19 (PVB19) is 70-85 % in adults worldwide. PVB19 is the etiologic agent of the fifth disease, is a cause of aplastic anemia, and can be associated with renal injury. We aimed to describe the cases of 4 patients with renal injury related to PVB19 primary infection, and to evaluate the seroprevalence of PVB19 and the incidence of PVB19 primary infection in patients undergoing a native kidney biopsy.
METHODS: Cases of PVB19 infection with renal injury were reviewed from the archives of the department of Nephrology. A systematic screening of anti-PVB19 IgG and IgM antibodies and viral DNA was performed in sera from 100 consecutive patients with a renal biopsy in 2017-2018.
RESULTS: The 4 patients with PVB19 infection-associated renal disease displayed: one lupus-like glomerulonephritis (GN) without lupus auto-antibodies, one minimal change disease with tubular necrosis, one secondary hemolytic and uremic syndrome and one membrano-proliferative GN. In the 100 patients biopsied, 67 had elevated anti-PVB19 IgG, among whom 8 had elevated IgM, without circulating viral DNA, without any particular renal pathological pattern. One additional patient showed a seroconversion at the time of renal biopsy, which revealed a class V lupus nephritis.
CONCLUSION: PVB19 primary infection can be associated with different renal diseases. The seroprevalence of PVB19 among patients with a renal biopsy is similar to the overall population, and primary infection is rarely documented (1%) after systematic screening. Whether PV19 is nephrotoxic, or triggers renal endothelial injury and immune activation, remains to be elucidated.
Health-related needs and barriers for forcibly displaced women: a systematic review
Authors: Jesuthasan Jenny , Witte Zara , Oertelt-Prigione Sabine 
Published: July 2019

Abstract
Introduction

Women represent a large proportion of the currently internationally displaced individuals. Due to gender roles, care duty, educational and economic imbalance their experiences and vulnerability during flight and relocation differ from that of men and children. The currently available information about their specific health-related needs and barriers to access is scarce and scattered.

Methods

We searched PubMed, Medline, EMBASE, Cochrane Library and Scopus to identify publications that explored the unique experiences of female refugees between 1 January 2008 and 30 June 2018. Publications needed to address the health needs of refugees, asylum seekers or displaced individuals, include at least 50% women in their study and employ a user-centered perspective, i.e. focus on the perspective of displaced individuals themselves and not health care providers. A framework of themes was identified and applied to all publications.

Results

We identified 1945 publications of which 13 could be included in the present review. Twelve of these publications employed qualitative and/or innovative methodology (e.g. ethnographies, index cards, photovoice). We identified five broad categories of health-related needs (immediate healthcare, communication, sultural/spiritual, social, economic), which are further detailed.

Conclusions

A concerted action providing information and culturally-sensitive care, while supporting language acquisition and economic empowerment is essential to improving the health status of female refugees. Transformative interventions need to address multiple axes of unequal access for female refugees to increase participation and overall health.
Attitudes towards early marriage and related factors among adolescent girls in Ethiopia
Authors: Gulema Hanna , Hamdan Ayah , Gingles Jessica , Friedman Lauren E. , Gelaye Bizu , Worku Amare , Worku Alemayehu , Berhane Yemane 
Published: July 2019

Abstract
Background

Early marriage, although it remains a common practice in sub-Saharan Africa, has detrimental effects on women’s sexual and reproductive health, social networks, and educational attainment. We sought to investigate the association between attitudes towards early marriage and marital status among adolescent girls in Ethiopia.

Methods

A cross-sectional study was conducted among 3,013 adolescent girls aged 13-17 years of age, living in four districts of the Oromia region of Ethiopia. Sociodemographic characteristics and were participants’ attitudes towards early marriage were measured using a pre-tested structured questionnaire.

Results

The prevalence of early marriage among adolescent girls was 8.3% and approximately 87.0% of girls had an overall positive attitude towards early marriage. Compared to adolescent girls who were unmarried, those who were married or living with a partner were less likely to believe they should have a say whether they want to marry or not (OR=0.32; 95%CI: 0.18-0.57), believe they should get to decide when to marry (OR=0.52; 0.31-0.88), and believe they should have the final decision over the decision to marry (OR=0.48; 0.32-0.73).

Conclusions

Interventions to prevent early marriage should strengthen women decision-making processes and promote women’s agency.
Recalibration of biochemistry measurements in a multinational cohort study: LIFE course study in CARdiovascular disease Epidemiology (LIFECARE)
Authors: Nang Ei Ei Khaing , Ng Sheryl HX , Shafiq Mahham , Tan Chuen Seng , Sy Rody , Fong Alan , Vathesatogkit Prin , Adam John , Llanes Elmer , Hian Sim Kui , Reganit Paul , Wong Kung Yee , Woodward Mark , Tai E Shyong , Venkataraman Kavita 
Published: July 2019

Abstract
Background

Various cardiovascular biomarkers are used to assess and compare the risk of cardiovascular diseases across populations. However, artefactual variations due to the use of different laboratories may make these comparisons invalid. This work describes the inter-laboratory variations in a multi-country cohort, LIFECARE, and the use of recalibration to a reference laboratory to minimise this variability.

Methods

LIFECARE is a cohort of 10,479 participants recruited from Indonesia, Malaysia, Philippines and Thailand between 2008 and 2011, with blood samples analysed at country-specific laboratories(n=4). Thailand was the designated reference laboratory. The measurements from each laboratory were compared against the reference laboratory using a common set of samples analysed at all laboratories, using the MethComp package in R. Laboratory values for cohort participants were recalibrated using the equation generated by the package, if large, statistically significant differences were observed during the comparison.

Results

Glucose, total cholesterol, HDL cholesterol, LDL cholesterol and triglyceride measurements were reported for all four countries. Cholesterol and HDL from all laboratories required recalibration while glucose did not. Recalibration altered the proportions of the population at risk substantially, with prevalence of high cholesterol changing from 56.3% to 75.0% in Malaysia, 52.1% to 37.5% in Indonesia and 31.3% to 22.7% in Philippines. Prevalence of low HDL was similarly altered.

Conclusion

There was significant variation in serum lipid levels measured by different laboratories, leading to variations in estimates of population at risk. Recalibration to a reference laboratory can overcome this variability and facilitate meaningful comparisons of laboratory data across countries.
Confirmatory factor analysis of the Evidence-Based Practice Attitude Scale in a large and representative sample of Child and Adolescent Mental Health practitioners: Is the use of a total scale score justified?
Authors: Santesson Anna , Jarbin Håkan , Holmberg Robert , Perrin Sean , Bäckström Martin 
Published: July 2019

Abstract
Background: High-quality instruments are essential for implementation practitioners and researcher; only valid and reliable measures can confidently and consistently measure what they are intended to measure. Psychometrically sound and pragmatic measures have the potential to guide the tailoring and evaluation of an implementation program thus contributing to the knowledge base of implementation science. The Evidence-Based Practice Attitude Scale (EBPAS) is one of few implementation instruments with strong psychometric qualities and is widely used to measure attitudes toward Evidence-Based Practice (EBP) in implementation practice and research. The Multidimensionality of the EBPAS has repeatedly been supported. Whether all of the fifteen individual items contribute to the EBPAS total scale beyond their subscales (Appeal, Requirements, Openness and Divergence) has yet to be proven. In addition, the Divergence subscale has been questioned because of low correlation with the other subscales and low inter-item correlations.
Methods: The factor structure of the EBPAS was investigated in a child- and adolescent psychiatry setting (N=925, 62%, n =570) using first- and second-order Confirmatory factor analysis, thus replicating Aarons' initial studies. In addition, the present study is the first to use a bifactor model in a large and representative sample.
Results: The EBPAS was supported as a multifaceted instrument. The EBPAS had a rather strong general factor supporting the use of the inventory as a single measure of attitudes. There were still four dimensions of attitude after the general factor was accounted for. All items, including the items of the Divergence factor, contributed to general attitude as well as to their specific construct.
Conclusions: The present study is the first to show that it is justified to use and interpret the EBPAS as an instrument measuring global as well as specific attitudes toward EBP. Sum score and subscales scores can be used in implementation research and practice.
Dilemma of the differential diagnosis of hilar cholangiocarcinoma and benign diseases:a single-center retrospective study
Authors: Pang Liwei 
Published: July 2019

Abstract
Objective

Hilar cholangiocarcinoma, which lacks specific clinical manifestations, remains very difficult to distinguish from benign disease. This distinction is further complicated by the complex hilar anatomy. We conducted the present study to evaluate the differential diagnosis of these conditions.

Methods

Sixty-five patients underwent resection surgery for suspected hilar cholangiocarcinoma between January 2011 and October 2018. Following a postoperative pathology analysis, all patients were divided into 2 groups: malignant group (54 patients with HCCA) and benign group (11 cases with benign lesions). The patients' clinical data, including general demographics (sex, age), clinical manifestations (jaundice, abdominal discomfort, fever, weight loss), laboratory data [alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALKP), total bilirubin (BILT), indirect bilirubin (BILD), carbohydrate antigen (CA) 19-9, carcinoembryonic antigen (CEA)], and imaging findings, were included in a retrospective analysis.

Results

Compared to the benign group, the malignant group had a significantly higher median age and serum CA19-9, CEA, ALT, BILT, and BILD levels (P <0.05). By contrast, the groups did not differ significantly in terms of the sex distribution, clinical manifestations, serum levels of AST and ALKP, and imaging findings. In a receiver operating characteristic curve analysis, we identified a CA19-1 cut-off point of 233.15 U/ml for the differential diagnosis, with a sensitivity of 56% and specificity of 91%. Furthermore, we identified a CEA cut-off point of 2.98 ng/ml for the differential diagnosis, with a sensitivity of 61% and specificity of 90%.

Conclusion

The differential diagnosis of HCCA and benign hilar lesions remains difficult. However, we found that patients with HCCA tended to have an older age at onset and higher serum levels of CA19-9, CEA, BILT, ALT, and BILD. Furthermore, patients with a serum CA19-9 level >233.15 U/ml and CEA level >2.98 ng/ml are more likely to have malignant disease.
Helicobacter pylori infection and increased diabetes prevalence were the risks of colorectal adenoma: a systematic review and meta-analysis
Authors: Wang Horng-Yuan , Lin Ying-Chun , Chen Chieh-Chang , Chen Ming-Jen , Wu Ming-Shiang , Liu Chun-Jen , Huang Chun-Ta , Yang Horng‐Woei , Shih Shou-Chuan , Yu Lo-Yip , Kuo Yang-Che , Hu Kuang-Chun 
Published: July 2019

Abstract
Background

Helicobacter pylori (H. Pylori) infection and hyperglycemia may be associated with an increased risk of colorectal neoplasm. However these two factors affect colorectal neoplasm remain controversial. We aimed to carry out a meta-analysis to evaluate the study population diabetes prevalence rate and H. pylori infection rate with colorectal adenoma risk.

Methods

We conducted a systemic research through English databases for medical reports. We also recorded the diabetes prevalence and H. pylori infection prevalence in each study. We classified these studies into 4 subgroups as their background population diabetes prevalence < 6%(Group 1); between 6 to 8%(Group 2); between 8 to 10%(Group 3) and more than 10%(Group 4). The random effects model had used to calculate pooled prevalence estimates with 95% confidence interval [CI].

Results

Twenty seven studies were finally eligible for meta-analysis. The random-effects model of meta-analysis was chosen, showing pooled odds ratio (OR) equal to 1.51 (95% CI 1.39–1.63). The subgroup meta-analyses showed in Group 1 the H. pylori infection associated colorectal adenoma risk OR was 1.24 (95% CI 0.86–1.78). As diabetes rate exceed 6%, the H. pylori infection became more significant increased risk of colorectal adenoma (Group 2: OR 2.16 (95% CI 1.61–2.91); Group 3: OR 1.40 (95% CI 1.24–1.57); Group 4: OR 1.52 (95% CI 1.46–1.57)).

Conclusions

The results of this meta-analysis showed DM prevalence would affect the risk factor of colorectal adenoma with H. pylori infection.