bluuebunny/crossref_metadata_2025_split huggingface dataset:
Social isolation uncovers a brain-wide circuit underlying context-dependent territory-covering micturition behavior
Authors: Hyun Minsuk , Taranda Julian , Radeljic Gianna , Miner Lauren , Wang Wengang , Ochandarena Nicole , Huang Kee Wui , Osten Pavel , Sabatini Bernardo 
Published: October 2019

AbstractThe controlled and volitional release of urine, or micturition, serves a fundamental physiological function and, in many species, is critical for social communication. In mice, the decision to release urine is modulated by external and internal factors such as environmental stimuli and social history and is transmitted to the spinal cord via the pontine micturition center (PMC). The neural pathways by which social experience and sensory stimuli interact to control PMC activity and regulate micturition are unclear. Here we establish a behavioral paradigm in which mice, depending on their strain, social experience, and immediate sensory context, display either high or low territory-covering micturition (TCM). We demonstrate that social context is represented by coordinated global activity changes in the urination network upstream of the PMC, whereas sensory context is represented by the activation of discrete nodes in the network. Furthermore, we show that the lateral hypothalamic area (LHA), which is directly upstream of PMC, is a key node that can switch micturition behavior between high and low TCM modes.
Stress-mediated convergence of splicing landscapes in male and female Rock Doves
Authors: Lang Andrew S , Austin Suzanne H , Harris Rayna M , Calisi Rebecca M , MacManes Matthew D 
Published: October 2019

AbstractBackgroundThe process of alternative splicing provides a unique mechanism by which eukaryotes are able to produce numerous protein products from the same gene. Heightened variability in the proteome has been thought to potentiate increased behavioral complexity and response flexibility to environmental stimuli, thus contributing to more refined traits on which natural and sexual selection can act. While it has been long known that various forms of environmental stress can negatively affect sexual behavior and reproduction, we know little of how stress can affect the alternative splicing associated with these events, and less still about how splicing may differ between sexes. Using the model of the rock dove (Columba livia), our team previously uncovered sexual dimorphism in the basal and stress-responsive gene transcription of a biological system necessary for facilitating sexual behavior and reproduction, the hypothlamic-pituitary-gonadal (HPG) axis. In this study, we delve further into understanding the mechanistic underpinnings of how changes in the environment can affect reproduction by testing the alternative splicing response of the HPG axis to an external stressor in both sexes.ResultsThis study reveals dramatic baseline differences in HPG alternative splicing between males and females. However, post submitting subjects to a restraint stress paradigm, we found a significant reduction in these differences between the sexes. In both stress and control treatments, we identified a higher incidence of splicing activity in the pituitary in both sexes as compared to other tissues. Of these splicing events, the core exon event is the most abundant form of splicing and more frequently occurs in the coding regions of the gene. Overall, we observed less splicing activity in the 3’UTR end of transcripts than the 5’UTR or coding regions.ConclusionsOur results provide vital new insight into sex-specific aspects of the stress response on the HPG axis at an unprecedented proximate level. Males and females uniquely respond to stress, yet exhibit splicing patterns suggesting a convergent, optimal splicing landscape for stress response. This information has the potential to inform evolutionary theory as well as the development of highly-specific drug targets for stress-induced reproductive dysfunction.
Keeping it clean: the cell culture quality control experience at the National Center for Advancing Translational Sciences
Authors: Roth Jacob S. , Lee Tobie D. , Cheff Dorian M. , Gosztyla Maya L. , Asawa Rosita , Danchik Carina , Michael Sam , Simeonov Anton , Klumpp-Thomas Carleen , Wilson Kelli , Hall Matthew D. 
Published: October 2019

AbstractQuality control monitoring of cell lines utilized in biomedical research is of critical importance, and is critical for reproducibility of data. Two key pitfalls in tissue culture are 1) cell line authenticity, and 2) mycoplasma contamination. As a collaborative research institute, the National Center for Advancing Translational Sciences receives cell lines from a range of commercial and academic sources, that are adapted for high-throughput screening. Here, we describe the implementation of a routine NCATS-wide mycoplasma testing and short-tandem repeat (STR) testing for cell lines. Initial testing identified a >10% mycoplasma contamination rate, but the implementation of clearly defined protocols that included immediate destruction of contaminated cell lines wherever possible has led to a much-reduced mycoplasma contamination rate, and data for >2,000 cell line samples tested over 3 years, and case studies are provided. STR testing of 170 cell lines with established STR profiles revealed only 5 mis-identified cell lines received from external labs. The data collected over the three years since implementation of this systematic testing demonstrates the importance of continual vigilance for rapid identification of ‘problem’ cell lines, for ensuring reproducible data in translational science research.
Choosing the optimal population for a genome-wide association study: a simulation using whole-genome sequences from rice
Authors: Hamazaki Kosuke , Kajiya-Kanegae Hiromi , Yamasaki Masanori , Ebana Kaworu , Yabe Shiori , Nakagawa Hiroshi , Iwata Hiroyoshi 
Published: October 2019

ABSTRACTA genome-wide association study (GWAS) needs to have a suitable population. The factors that affect a GWAS, e.g. population structure, sample size, and sequence analysis and field testing costs, need to be considered. Mixture populations containing subpopulations of different genetic backgrounds may be suitable populations. We conducted simulation experiments to see if a population with high genetic diversity, e.g., a diversity panel, should be added to a target population, especially when the target population harbors small genetic diversity. The target population was 112 accessions ofOryza sativasubsp.japonica, mainly developed in Japan. We combined the target population with three populations that had higher genetic diversities. These were 100indicaaccessions, 100japonicaaccessions, and 100 accessions with various genetic backgrounds. The results showed that the GWAS power with a mixture population was generally higher than with a separate population. Also, the GWAS optimal population varied depending on the fixation indexFSTof the quantitative trait nucleotide (QTN) and its polymorphism of QTN in each population. When a QTN is polymorphic in a target population, a target population combined with a higher diversity population improves the QTN detection power. InvestigatingFSTand the expected heterozygosityHeas factors influencing the detection power, we showed that SNPs with highFSTor lowHeare less likely to be detected by GWAS with mixture populations. Sequenced/genotyped germplasm collections can improve the GWAS detection power by using a subset of them with a target population.Core ideas (3-5 impact statements, 85 char max for each)- Genome-wide association studies with mixture populations are expected to improve the detection power of novel genes due to the increase of the sample size although the influence of population structure is a concern.- When a quantitative trait nucleotide (QTN) is polymorphic in a target population, a combination of the target population and a population with higher diversity than the target population improves the detection power of the QTN.- We found that the fixation index (FST) and the expected heterozygosity (He) were strongly related to the detection power of QTNs.- Germplasm collections which have been already sequenced/genotyped are useful for improving the detection power of GWAS without any addition of sequence costs by using a subset of them with a target population.
Effects of potassium silicate on corrosion behavior of dacromet coating
Authors: Tabi Khashayar , Farzam Mansour , Zaarei Davood 
Published: October 2019

Purpose
Potassium silicate sealer was applied on solvent-cleaned, acid-pickled, dacromet-coated steel to improve its corrosion resistance. The purpose of this paper is to study the corrosion behavior of dacromet-coated steel.

Design/methodology/approach
Potassium silicate sealer was applied on solvent-cleaned, acid-pickled, dacromet-coated steel to improve its corrosion resistance. Electrochemical impedance spectroscopy (EIS), potentiodynamic polarization, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and salt spray were carried out. SEM was used to study the morphological appearance of the surface.

Findings
The EIS behavior indicated that solvent-cleaned dacromet-coated steel sealed with potassium silicate showed that the corrosion current density was 2.664E − 5 A.cm2 which was reduced to 8.752E − 6 A.cm2 and the corrosion rate, which was 2.264E − 2 mm.year−1, was reduced to 7.438E − 3 mm.year−1 in NaCl 3.5 wt.per cent. EIS was used in NaCl 3.5 wt.%, and the Bode plot characteristics showed that the corrosion protection of solvent-cleaned, dacromet-coated steel was enhanced when sealed with potassium silicate. The EDS results of salt-sprayed, solvent-cleaned samples after 10 days indicated that the main corrosion products are composed of SiO2, ZnO and Al2O3.

Research limitations/implications
The detection of Li element in EDS was not possible because of the device limitation.

Originality/value
The current paper provides new information about the sealing properties of potassium silicate and its effects on the corrosion resistance of dacromet coating, which is widely used in many industries such as the automobile industry.
Influence of big data and predictive analytics and social capital on performance of humanitarian supply chain
Authors: Jeble Shirish , Kumari Sneha , Venkatesh V.G. , Singh Manju 
Published: October 2019

PurposeThe purpose of this paper is threefold: first, to investigate the role of big data and predictive analytics (BDPA) and social capital on the performance of humanitarian supply chains (HSCs); second, to explore the different performance measurement frameworks and develop a conceptual model for an HSC context that can be used by humanitarian organizations; and third, to provide insights for future research direction.Design/methodology/approachAfter a detailed review of relevant literature, grounded in resource-based view and social capital theory, the paper proposes a conceptual model that depicts the influence of BDPA and social capital on the performance of an HSC.FindingsThe study deliberates that BDPA as a capability improves the effectiveness of humanitarian missions to achieve its goals. It uncovers the fact that social capital binds people, organization or a country to form a network and has a critical role in the form of monetary or non-monetary support in disaster management. Further, it argues that social capital combined with BDPA capability can result in a better HSC performance.Research limitations/implicationsThe proposed model integrating BDPA and social capital for HSC performance is conceptual and it needs to be empirically validated.Practical implicationsOrganizations and practitioners may use this framework by mobilizing social capital, BDPA to enhance their abilities to help victims of calamities.Social implicationsFindings from study can help improve coordination among different stakeholders in HSC, effectiveness of humanitarian operations, which means lives saved and faster reconstruction process after disaster. Second, by implementing performance measurements framework recommended by study, donors and other stakeholders will get much desired transparency at each stage of HSCs.Originality/valueThe findings contribute to the missing link of social capital and BDPA to the existing performance of HSC literature, finally leading to a better HSC performance.
A novel hybrid series stacked differential fractal inductor for MMIC applications
Authors: Tumma Sunil Kumar , Nistala Bheema Rao 
Published: October 2019

Purpose
The purpose of this paper is to design an on-chip inductor with high inductance, high-quality factor and high self-resonance frequency for the equivalent on-chip area using fractal curves.

Design/methodology/approach
A novel hybrid series stacked differential fractal inductor using Hilbert and Sierpinski fractal curves is proposed with two different layers connected in series using vias. The inductor is implemented in Sonnet EM simulator using 180 nm CMOS standard process technology.

Findings
The proposed inductor reduces the parasitic capacitance and negative mutual inductance between the adjacent layers with significant improvement in overall inductance, quality factor and self-resonance frequency when compared with conventional series stacked fractal inductors.

Research limitations/implications
The fractal inductor is used to create high inductance in the single-layer process, but access to multilayers is restricted owing to unusual and expensive fabrication processes.

Practical implications
The proposed inductor can be used in implementation of low noise amplifier, voltage controlled oscillators and power amplifiers.

Originality/value
This paper introduces a combination of two fractal curves to implement a hybrid fractal inductor that enhances the performance of the inductor.
Ethics and crisis translation: insights from the work of Paul Ricoeur
Authors: O’Mathúna Dónal P. , Hunt Matthew R. 
Published: October 2019

PurposeThe purpose of this paper is to explore the ethical dimensions of crisis translation through the lenses of Paul Ricoeur’s philosophical scholarship. In particular, his work on both translation and ethics will be examined in order to draw practical applications for those involved in humanitarian action.Design/methodology/approachThe authors identified relevant themes in the work of renowned philosopher Paul Ricoeur and used philosophical analysis to apply them to ethical issues in crisis translation.FindingsPaul Ricoeur was one of the leading philosophers in the twentieth century, writing on a wide variety of topics. From these, his work on translation and on ethics provided suitable ways to examine ethical issues in crisis translation. In particular, his concept of “linguistic hospitality” provides an important lens through which translation ethics can be examined. In addition, Ricoeur’s approach to ethics emphasised relational and justice dimensions which are crucial to examine in humanitarian settings.Practical implicationsWhile the findings are conceptual, they have many practical implications for how translation is approached in humanitarian crises. The focus on justice in Ricoeur’s approach has implications for policy and practice and serves to ensure that translation is available for all affected communities and that all groups are included in discussions around humanitarian responses.Social implicationsRicoeur’s work provides important insights into both translation and ethics that have significant social implications. His ideas highlight the personal and emotional aspects of translation and ethics, and point to their relational character. His openness to others provides an important basis for building trust and promoting dignity even in difficult humanitarian settings.Originality/valueRicoeur’s ethics points to the importance of persons and their relationships, reminding responders that translation is not just a mechanical exercise. This approach fosters an interest in and openness to others and their languages, which can promote respect towards those being helped in humanitarian crises.
Governance of systemic risks for disaster prevention and mitigation
Authors: Schweizer Pia-Johanna , Renn Ortwin 
Published: October 2019

Purpose
Systemic risks originate in tightly coupled systems. They are characterised by complexity, transboundary cascading effects, non-linear stochastic developments, tipping points, and lag in perception and regulation. Disasters need to be analysed in the context of vulnerabilities of infrastructure, industrial activities, structural developments and behavioural patterns which amplify or attenuate the impact of hazards. In particular, disasters are triggered by chains of events that often amplify and also multiply damages. The paper aims to discuss these issues.

Design/methodology/approach
The paper applies the concept of systemic risks to disasters more precisely to the combination of natural and human-induced disasters. The paper refers to the International Risk Governance Council’s Risk Governance Framework and applies this framework to the systemic aspects of disaster risks.

Findings
The paper maps out strategies for inclusive governance of systemic risks for disaster prevention and mitigation. Furthermore, the paper highlights policy implications of these strategies and calls out for an integrated, inclusive and adaptive management approach for the systemic aspects of disaster risks.

Originality/value
The paper fulfils the identified need to analyse disaster risks in the context of vulnerabilities of infrastructure, industrial activities, structural developments and behavioural patterns which amplify or attenuate the impact of hazards.
Empowering the insured under health insurance: a case study in center of Iran
Authors: Pakdaman Mohsen , Geravandi Sara , Hejazi Ali , Salehi Mobin , Davoodifar Mahboobeh 
Published: October 2019

PurposeCurrently, the health system is a treatment-oriented system focused on service providers. In this system, the main focus is on the health market, with little attention on insured. One way to get out of existing conditions is to empower the insured in order to involve them actively in maintaining and improving health. The paper aims to discuss these issues.Design/methodology/approachThis qualitative study was done using the content analysis method. Based on the purposive sampling method and theoretical saturation criterion, 24 individuals including 12 health insurance experts and 12 insured participated in the study in 2018. The semi-structured interview method was used to collect data. Data were analyzed using MAXQDA10 software.FindingsHaving analyzed the interviews, 750 codes were obtained. These codes were categorized into two categories of “insurance experts” and “insured” and ten subcategories of “informing and educating, cost reduction, intersectional activities, expectations from the insured, services package, access to services, inability to pay costs, participation, and expectations from the insurance organization.”Originality/valueThis qualitative study was conducted to assess and determine the effective strategies for empowering the insured under health insurance. The results of this study are helpful to the health insurance organizations and health decision makers to detect the effective ways to develop the quality of insurance services, improve the status of insured, and increase access to health care goods and services.
Application of lean manufacturing in automotive manufacturing unit
Authors: Singh Jagmeet , Singh Harwinder 
Published: October 2019

PurposeThis paper aims to apply lean manufacturing using value stream mapping (VSM) in the manufacturing organization (automotive suspension and fastening components section). To validate the VSM approach, simulation has been done.Design/methodology/approachVSM approach has been used to implement lean stages in the U-bolt section. Current state map was prepared and future state map has been implemented. Further, simulation has been done to compare current state and future state maps.FindingsIt has been found that there is 87.59% reduction in cycle time (C/T), 76.47% reduction in work-in-process (WIP) inventory, 95.41% reduction in production lead time, 66.08% increase in value added (VA) ratio, 95.78% reduction in non-value added (NVA) time, 57.14% reduction in the number of operators and 70.67% reduction in change over (C/O) time for the U-bolt section. Simulated and VSM of current and future state has observed error of 5 s.Research limitations/implicationsThis study is based on automotive manufacturing industry situated in northern part of India. The outcome of this study is applicable only to the selected product of the manufacturing company.Originality/valueThis paper deals with case study part which reflects the true picture of implementation of Lean manufacturing (LM) tools in the organization.
A longitudinal exploratory study of ISO 9001 certification abandonment in small- and medium-sized enterprises
Authors: Zimon Dominik , Dellana Scott 
Published: October 2019

Purpose
The purpose of this paper is to explore the expectations for ISO 9001 certification in a group of small- and medium-sized enterprises, then to assess the reasons for their subsequent abandonment of the certification and the long-term impacts of that decision.

Design/methodology/approach
Data for this study come from longitudinal case studies involving a series of questionnaires, in-depth on-site interviews and informal conversations with top managers of 22 firms in the heating technology service industry in Poland. The research process was conducted in three stages. The first stage occurred in the Summer of 2008 in which a broad analysis was conducted of the impact of ISO 9001 certification on the functioning of the organizations. The second stage was conducted in 2012, to gather the information about the organizations that subsequently abandoned their ISO 9001 certification. The third stage was carried out in 2016 to examine the longer-term impact of ISO 9001 certification abandonment in these organizations.

Findings
The main motivation for study participants obtaining ISO 9001 certification centred around market visibility and gaining new customers rather than on lowering operating costs or improving quality. Managers also expected certification to improve organization documentation. The decision to abandon certification had to do with lack of time for improvement efforts and questionable cost-benefit that could lead to higher prices. Certification was considered unnecessary to achieve the goals of the organization. In retrospect, only a small percentage (20 per cent) of small- and medium-sized enterprises in the sample group felt that abandoning ISO 9001 certification was largely the correct decision. A majority (53 per cent) thought it was largely a regrettable decision.

Research limitations/implications
The research is based on a limited number of case studies for smaller organizations in one purchasing group in industry rather than on a large cross-section of different organizations sizes and types. Therefore, the ability to draw statistical inferences is limited and the results may not generalize to other settings. However, this appears to be one of the few studies of its kind on voluntary abandonment of the certification.

Practical implications
It is important for business managers to understand not only the reasons supporting initial certification, but the rationale that organizations cite for abandonment of the certification and the long-term impacts of that decision.

Originality/value
A study of voluntary ISO 9001 certification abandonment appears to be relatively absent in the literature. This research opens a new area of research into the assessment of potential consequences of abandoning the certification.