bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Substitution Pro32Thr on the Interaction between Dimer Subunits of Human Phosphatase ITPA
Authors: Dushanov Ermuhammad B. , Koltovaya Natalia A. 
Published: March 2019

Background:
Cells have specific enzymes (nucleoside triphosphate pyrophosphohydrolase)
that hydrolyze non-canonic nucleoside triphosphates into nucleoside monophosphophates and pyrophosphate,
thus removing them from the metabolic processes. This class of enzymes includes inosine
triphosphate pyrophosphatase (ITPA) which has specificity to ITP, dITP, XTP and dXTP.

Objective:
The mutation (94C→A) rather often occurs in humans and can affect the sensitivity of patients
to medicines. This mutation leads to a Pro32Thr substitution in the human ITPA protein. The
mechanism for the inactivating effect of the mutation is unknown yet.

Methods:
Molecular modeling of the polymorphic form of inosine triphosphate pyrophosphohydrolase
Р32Т-hITPA showing the greatest decrease in the enzyme activity is performed. The analysis is
given for four dimer variants: wild-type (P32/P32) and mutant (T32/T32) homodimers and two mutant
heterodimers (Р32/Т32 and Т32/Р32).

Results:
The analysis does not show the motion of the loop between α2 and β2 where mutation localized.
Thus, the hypothesis of the flipped-out hydrophobic residue and subsequent of protein degradation
have not been confirmed. Dimer displacements were much higher than subunit displacements.
The analysis of hydrogen bonds between subunits shows that there are the more stable hydrogen
bonds in the wild-type homodimer and fewer in the mutant homodimer, while heterodimers have intermediate
stability.

Conclusion:
The results confirm the assumption of possible weakening of bonds between the mutant
subunits
Automated Post-Column Sample Manipulation Prior to Detection in Liquid Chromatography: A Review of Pharmaceutical and Bioanalytical Applications
Authors: Manousi Natalia , K. Zacharis Constantinos 
Published: March 2019

:
Automated post-column sample manipulation is undoubtedly one of the most challenging
approaches in liquid chromatography for the improvement of method selectivity and sensitivity. With
the post-column analyte derivatization being the most-abundant approach approach of this category,
other strategies typically comprise post-column infusion of internal standard or other reagents prior
to mass spectrometric detection to enhance the ionization efficiency of the analyte or to compensate
the ion suppression/enhancement.

:
In this review, on-line post column methodologies focused on the quality control of pharmaceuticals
and biomedical applications will be presented and discussed. Emphasis will be given on the
automation capabilities of such systems.
The Thiol-Disulfide Homeostasis and Coenzyme Q10 in Conjunction with Vitamin E Effect on Retinopathy Prematurity
Authors: Akdogan Muberra , Ustundag Yasemin , Akdağ Arzu , Neselioglu Salim , Erel Ozcan 
Published: March 2019

Purpose:
This study was performed to determine whether one drop of topical administration of Coqun® (Coenzyme Q10 and Vitamin E)-a potent antioxidant-twice a day has any effect on the thiol-disulphide homeostasis-a novel oxidative stress marker in the Retinopathy Of Prematurity (ROP) disease course.

Methods:
This was a prospective observational study comprising 28 infants with ROP at stage 2 and higher who followed up at the paediatric intensive care unit. Ferric reducing power of plasma (FRAP), albumin, ischemia-modified albumin (IMA) and thiol disulphide homeostasis levels were studied in the infants before and two weeks after Coqun® treatment.

Results:
The mean gestational age was 27 (24–32) weeks, the mean birth weight was 1,012±326 g and the mean duration of care in an incubator was 64±23 days. FRAP levels were 0.91±0.17 μmol/L, IMAs were 0.85±0.29, native thiols were 248±38.9 μmol/L and total thiols were 284±39.2 μmol/L, respectively, at the beginning of therapy.
FRAP levels 0.79±0.21(p= 0.006) μmol/L, IMAs 0.73±0.36(p = 0.096), native thiols 262±42.6(p = 0.164) μmol/L and total thiols 291±43.6(p = 0.344) μmol/L showed no difference after two weeks of therapy.

Conclusion:
Thiol disulphide homeostasis levels do not change with Coqun® therapy during ROP course.
A Revision Arthrodesis of Severe Charcot Foot with Intramedullary Nail: a Case Report and Literature Review
Authors: Zaizi Abderrahim , Benomar Hicham Ahmed , Bakayan Mohamed Said , Krimch Omar , Lamrani Moulay Omar , Berrada Mohammed Saleh 
Published: March 2019

Diabetic Charcot foot is characterized by varying degrees of bone and joint disorganization secondary to underlying neuropathy, perturbations of bone metabolism and trauma. Offloading is the most important initial treatment recommendation. Surgery can be helpful in early stages involving acute fractures of the foot or ankle or in later stages when offloading is ineffective. Incorrect diagnosis and improper treatment often result in the extremity having to be amputated.

There are several surgical procedures accepted in Charcot foot surgery. Their goal is to obtain a plantigrade foot and prevent recurrent ulcerations. Arthrodesis is a well-known surgical procedure that addresses severe joint derangement through a surgically induced bony fusion. In Charcot foot, arthrodesis is usually indicated when there is significant skeletal instability. This procedure can be done by internal or external fixation.
Erzurum Bölgesinde Yetişen Betula pendula Yapraklarının Antioksidan Kapasitelerinin Belirlenmesi ve Kemometrik Karakterizasyonu
Authors: ŞENOL Onur 
Published: March 2019

Bu çalışmada, Erzurum bölgesinin karakteristik bitki örtüsü içerisinde yer alan Huş Ağacına ait farklı yerleşimlerden alınan yaprak örnekleri farklı in vitro antioksidan kapasite yöntemleri ile incelenmiş ve kemometrik karakterizasyonu yapılmıştır. Uzundere, Horasan ve Şenkaya bölgelerinde mayıs-temmuz aralığında toplanan örnekler kurutulmuş ve etanol ekstreleri çıkarılmıştır. Bakır II iyonu İndirgeyici Antioksidan Kapasite (CUPRAC), Demir III iyonu İndirgeyici Antioksidan Kapasite (FRAP) ve Difenil pikril Hidrazin (DPPH) yöntemleri için gerçekleştirilen analizlere bütün örnekler pozitif yanıt vermişlerdir. En yüksek antioksidan kapasite değerlerine Şenkaya bölgesinden elde edilen örneklerle ulaşılmıştır. Elde edilen değerler bilgisayar algoritmaları yardımıyla kemometrik olarak incelendiğinde; sonuçların anlamlı farklılık gösterdiği ve her bölgede rakım değişimiyle pozitif korelasyon gösteren farklılıklar tespit edilmiştir. Yapılan çalışma vasıtasıyla antioksidan kapasite değerleri ile coğrafi karakterizasyonun gerçekleştirilebileceği ve rakımsal farklılıkların Huş ağacı yapraklarının fenolik ve flavanoid içeriğine anlamlı etki yaptığı sonucuna varılmıştır. Huş ağacı bitkisinin antioksidan kapasitesi sebebiyle özellikle kanser ve kozmetik gibi önemli alanlarda kullanılabileceği düşünülmektedir.
PENGARUH LITERASI EKONOMI DAN KONTROL DIRI TERHADAP PERILAKU PEMBELIAN IMPULSIF UNTUK PRODUK FASHION
Authors: Rozaini Noni , Ginting Bismi Aditya 
Published: March 2019

Permasalahan dalam penelitian ini rendahnya kontrol diri mahasiswa Prodi Pendidikan Bisnis Stambuk 2014 Unimed. Penelitian ini bertujuan untuk mengetahui pengaruh literasi ekonomi dan kontrol diri terhadap perilaku pembelian impulsif mahasiswa Prodi Pendidikan Bisnis Unimed. Populasi dalam penelitian ini adalah seluruh mahasiswa Prodi Pendidikan Bisnis stambuk 2014 Unimed yang berjumlah 76 mahasiswa dan sampel sebanyak 76 mahasiswa dengan teknik total sampling. Teknik pengambilan data menggunakan observasi, dokumentasi, tes dan penyebaran angket. Uji validitas dengan menggunakan teknik analisis product moment dan reliabilitas angket menggunakan rumus Alpha Croanbanch. Persamaan regresi linear berganda menunjukkan Y= 9,270 - 0,017 (X1) - 0,787 (X2) + 0,05. Hasil uji t (parsial) menunjukkan literasi ekonomi tidak terdapat pengaruh negatif dan signifikan terhadap perilaku pembelian impulsif. Kontrol diri berpengaruh negatif dan signifikan terhadap perilaku pembelian impulsif. Secara simultan, kategori literasi ekonomi dan kontrol diri berpengaruh negatif dan signifikan terhadap perilaku pembelian impulsif. Hasil uji koefisien determinasi (R2) sebesar 37,1% dan sisanya dipengaruhi oleh faktor lain di luar penelitian.Kata Kunci : Literasi Ekonomi, Kontrol Diri, Perilaku Pembelian Impulsif.
PENGARUH PENGETAHUAN KEWIRAUSAHAAN DAN PENDAPATAN ORANGTUA TERHADAP MINAT BERWIRAUSAHA SISWA KELAS XII PEMASARAN SMK NEGERI 7 MEDAN T.A 2018/2019
Authors: Nasution M. Fachry , Panggabean Sry Melva 
Published: March 2019

Penelitian ini bertujuan untuk mengetahui tingkat minat berwirausaha siswa di kelas XII Pemasaran SMK Negeri 7 Medan tahun ajaran 2018/2019. Penelitian ini adalah jenis penelitian Expos-Facto dengan metode kuantitatif.. Populasi dalam penelitian ini seluruh siswa kelas XII Pemasaran SMK Negeri 7 Medan yang terdiri dari 2 kelas. Sampel yang digunakan adalah seluruhnya dengan cara teknik total sampling yang terdiri dari 65 siswa. Instrumen yang digunakan adalah angket bentuk pilihan berganda dan pernyataan dengan menggunakan skala likert. Hasil analisis data perhitungan regresi linear berganda diperoleh persamaan garis linear Y = 21,267 + 0,338 X1 + 0,454 X2 . Secara parsial pengujian hipotesis dengan menggunakan statistik Uji-t untuk variabel X1 diperoleh thitung 4,209 dengan signifikan  = 0,000 dimana ttabel sebesar 1,669. Hasil pengujian ini menunjukkan bahwa thitung>ttabel (4,209>1,669) atau sig  = 0,000<0,05 dengan kata lain hipotesis diterima. Kemudia untuk variabel X2 diperoleh thitung>ttabel (5,393>1,669) atau sig  0,000<0,05 dengan demikian hipotesis diterima. Hasil pengujian hipotesis secara simultan diperoleh Fhitung = 56,214 menunjukkan bahwa Fhitung>Ftabel (56,214>3,15) dengan signifikansi  = 0,000<0,05 yang berarti bahwa hipotesis diterima, dimana ada pengaruh yang positif dan signifikan antara pengetahuan kewirausahaan dan pendapatan orangtua terhadap minat berwirausaha siswa kelas XII Pemasaran SMK Negeri 7 Medan tahun ajaran 2018/2019 yang berarti hipotesis ini dapat diterima. Kata Kunci : Pengetahuan Kewirausahaan, Pendapatan Orangtua, dan Minat Berwirausaha.
PENGARUH PENERAPAN KURIKULUM KKNI TERHADAP MINAT MENELITI MAHASISWA
Authors: Agusti Ivo Selvia , Sihotang Roberto Erdian 
Published: March 2019

Penelitian ini bertujuan untuk mengetahui pengaruh penerapan kurikulum KKNI ter-hadap minat meneliti mahasiswa Program Studi Pendidikan Bisnis Universitas Negeri Medan. Populasi dalam penelitian ini adalah seluruh mahasiswa Prodi Pendidikan Bisnis Stambuk 2016 UNIMED yang berjumlah 103 mahasiswa dan sampel sebanyak 82 mahasiswa dengan teknik solvin sampling. Teknik Pengambilan data menggunakan observasi, dokumentasi dan penyebaran angket. Uji validitas menggunakan teknik analisis product moment dan reliabili-tas angket menggunakan Cronbach Alpha. Hasil pengolahan data disimpulkan bahwa KKNI memiliki pengaruh positif dan sig-nifikan terhadap minat meneliti, yang ditunjukkan dari perhitungan regresi sederhana dimana didapatkan persamaan Y = 22,664 + 0,468 (X). Dan thitung > ttabel ( 9,908 > 1,664 ) dengan nila alpha 0,000 yang artinya dibawah (α)0,05.. Dan Hasil uji koefisien determinasi (R2) sebesar 54.5% .Pembahasan dalam penelitian diasumsikan adanya pengaruh faktor-faktor lain seperti persepsi lingkungan pelatihan penelitian, minat dalam penelitian, Konsep diri yang lebih tinggi (Glenn W. Lambie & Nicole Vaccaro, 2011) Kata Kunci : Kurikulum, Minat Meneliti
Effects of an enrofloxacin–silver sulfadiazine emulsion in the ears of rabbits with perforated tympanic membranes
Authors: Bateman Fiona L. , Kirejczyk Shannon G. M. , Stewart Georgina V. , Cutler Daniel C. , Quilling Laura L. , Howerth Elizabeth W. , Mayer Joerg 
Published: March 2019

Abstract

OBJECTIVE
To determine whether an enrofloxacin–silver sulfadiazine emulsion (ESS) labeled for treatment of otitis externa in dogs has ototoxic effects in rabbits following myringotomy.

ANIMALS
6 healthy adult New Zealand White rabbits.

PROCEDURES
Rabbits were anesthetized for brainstem auditory-evoked response (BAER) tests on day 0. Myringotomy was performed, and BAER testing was repeated. Saline (0.9% NaCl) solution and ESS were then instilled in the left and right middle ears, respectively, and BAER testing was repeated prior to recovery of rabbits from anesthesia. Application of assigned treatments was continued every 12 hours for 7 days, and rabbits were anesthetized for BAER testing on day 8. Rabbits were euthanized, and samples were collected for histologic (6 ears/treatment) and scanning electron microscopic (1 ear/treatment) examination.

RESULTS
Most hearing thresholds (11/12 ears) were subjectively increased after myringotomy, with BAER measurements ranging from 30 to 85 dB in both ears. All day 8 hearing thresholds exceeded baseline (premyringotomy) values; results ranged from 30 to 85 dB and 80 to > 95 dB (the upper test limit) in saline solution–treated and ESS-treated ears, respectively. All ESS-treated ears had heterophilic otitis externa, epithelial hyperplasia of the external ear canal, various degrees of mucoperiosteal edema, and periosteal new bone formation on histologic examination. Scanning electron microscopy revealed that most outer hair cells in the ESS-treated ear lacked stereocilia or were absent.

CONCLUSIONS AND CLINICAL RELEVANCE
Results supported that ESS has ototoxic effects in the middle ear of rabbits. Further research is needed to confirm these findings. Myringotomized laboratory rabbits may be useful to study ototoxicity of drugs used in human medicine.
Quantitative characterization of viscoelastic properties of synovial fluid from forelimb joints of orthopedically normal Thoroughbreds and warmblood horses
Authors: Tyrnenopoulou Panagiota C. , Rizos Eleftherios D. , Kritsepi-Konstantinou Maria , Papadopoulou Paraskevi L. , Patsikas Michail N. , Papazoglou Lysimachos G. , Aggeli Amalia , Diakakis Nikolaos E. 
Published: March 2019

Abstract

OBJECTIVE
To determine whether differences existed in the viscoelastic properties of synovial fluid samples from the metacarpophalangeal, intercarpal, and distal interphalangeal joints of orthopedically normal athletic horses.

ANIMALS
45 warmblood horses and 30 Thoroughbreds (age range, 4 to 16 years).

PROCEDURES
Synovial fluid samples were aseptically obtained via arthrocentesis from 1 metacarpophalangeal, intercarpal, and distal interphalangeal joint of each horse, and nucleated cell counts were performed. A commercial ELISA was used to measure sample hyaluronic acid concentrations, and full rheological characterization of samples was performed to measure the elastic or storage modulus G' and viscous or loss modulus G“ at 37.5°C (representing the body temperature of horses). Findings were compared among joints and between breed groups by means of ANOVA.

RESULTS
Significant differences in synovial fluid G' and G“ values were identified between Thoroughbreds and warmblood horses for the metacarpophalangeal joint, between the metacarpophalangeal and intercarpal joints of Thoroughbreds, and between the metacarpophalangeal and distal interphalangeal joints and intercarpal and distal interphalangeal joints of warmblood horses. No significant differences were identified between breed groups or among joints in synovial fluid hyaluronic concentrations or nucleated cell counts.

CONCLUSIONS AND CLINICAL RELEVANCE
Viscoelastic properties of the forelimb joints of orthopedically normal Thoroughbreds and warmblood horses differed within and between these 2 groups, mainly as a function of the evaluated joint. To the authors' knowledge, this was the first study of its kind, and additional research is warranted to better understand the viscoelastic properties of synovial fluid in horses to optimize their locomotive function.
Usefulness of focused cardiac ultrasonography for predicting fluid responsiveness in conscious, spontaneously breathing dogs
Authors: Oricco Stefano , Rabozzi Roberto , Meneghini Caterina , Franci Paolo 
Published: March 2019

Abstract

OBJECTIVE
To evaluate the diagnostic usefulness of focused cardiac ultrasonography and selected echocardiographic variables for predicting fluid responsiveness in conscious, spontaneously breathing dogs with various clinical conditions.

ANIMALS
26 dogs (15 males and 11 females) with a median age of 84 months (range, 12 to 360 months) and median body weight of 8 kg (range, 2 to 35 kg) referred for various clinical conditions.

PROCEDURES
Left ventricular end-diastolic internal diameter normalized to body weight (LVIDDn), left ventricular volume score (LVVS), left ventricular end-diastolic volume index (EDVI), aortic velocity time integral (VTIAo), and aortic peak flow velocity (VmaxAo) were echocardiographically measured before and after IV administration of a bolus of lactated Ringer solution (4 mL/kg) over a 1-minute period. Dogs were classified on the basis of the observed change in aortic stroke volume following fluid administration as responders (≥ 15%) or nonresponders (< 15%) to fluid administration. Receiver operating characteristic curves were generated for the ability of LVVS, LVIDDn, EDVI, VTIAo, and VmaxAo to predict responder status.

RESULTS
13 dogs were classified as responders and 13 as nonresponders. Areas under the receiver operating characteristic curves (95% confidence intervals) for predicting fluid responsiveness were as follows: VTIAo, 0.91 (0.74 to 0.99); LVIDDn, 0.85 (0.66 to 0.96); EDVI, 0.85 (0.65 to 0.96); LVVS, 0.85 (0.65 to 0.96); and VmaxAo, 0.75 (0.54 to 0.90).

CONCLUSIONS AND CLINICAL RELEVANCE
The evaluated echocardiographic variables were useful for noninvasive prediction of fluid responsiveness in conscious dogs and could be valuable for informing clinical decisions regarding fluid therapy.
Evaluation of liver and spleen stiffness of healthy dogs by use of two-dimensional shear wave elastography
Authors: Tamura Masahiro , Ohta Hiroshi , Nisa Khoirun , Osuga Tatsuyuki , Sasaki Noboru , Morishita Keitaro , Takiguchi Mitsuyoshi 
Published: March 2019

Abstract

OBJECTIVE
To assess liver and spleen stiffness in healthy dogs by use of a novel 2-D shear wave elastography (SWE) technique and to investigate the repeatability and reproducibility of the technique.

ANIMALS
8 healthy adult Beagles.

PROCEDURES
2-D SWE was performed on each dog to assess liver and spleen stiffness. Repeatability (intraday variability) and reproducibility (interday variability) of 2-D SWE were investigated. For all 8 dogs, 2-D SWE was performed 3 times in 1 day (4-hour intervals) and on 3 separate days (1-week interval). Data were expressed as mean ± SD values for shear wave velocity and the Young modulus in the liver and spleen. Intraday and interday coefficients of variation were assessed for all variables.

RESULTS
Mean ± SD shear wave velocity obtained for the liver and spleen was 1.51 ± 0.08 m/s and 2.18 ± 0.27 m/s, respectively. Mean value for the Young modulus obtained for the liver and spleen was 6.93 ± 0.79 kPa and 14.66 ± 3.79 kPa, respectively. Elasticity values were significantly higher for the spleen than for the liver. Intraday and interday coefficients of variation for all variables were < 25% (range, 3.90% to 20.70%).

CONCLUSIONS AND CLINICAL RELEVANCE
2-D SWE was a feasible technique for assessing liver and spleen stiffness of healthy dogs. Future studies on the application of 2-D SWE for dogs with chronic hepatitis, cirrhosis, and portal hypertension are needed to evaluate the clinical applicability of 2-D SWE.
In vitro evaluation of a medial approach for intra-articular injection of the tarsometatarsal joints of horses
Authors: Canonici Paolo , Beccati Francesca , Canonici Fernando , Pepe Marco 
Published: March 2019

Abstract

OBJECTIVE
To develop a reliable method for injection of the tarsometatarsal (TMT) joint of horses through a medial approach and to characterize anatomy of the synovial recesses of the TMT joint with CT.

SAMPLE
13 pairs of fresh cadaveric equine tarsi.

PROCEDURES
TMT joints were assessed with radiography and CT. Twelve pairs of TMT joints were assigned to an experienced veterinarian (8 joints) or veterinary student (16 joints) for injection with contrast medium through a medial or a lateral approach. Every TMT joint was then reexamined radiographically and with CT to assess diffusion of contrast medium. The number of attempts for the lateral and medial approaches between and within investigators was evaluated, which included evaluation of the diffusion of contrast solution in relation to the approach used.

RESULTS
All 24 TMT joints were successfully injected, as confirmed with radiography and CT. There was no significant difference in the number of attempts between the medial and lateral approaches and between investigators. No significant difference was detected in the distribution of contrast medium in each synovial recess between the medial and lateral approaches.

CONCLUSIONS AND CLINICAL RELEVANCE
The medial approach described here for injection of the TMT joint of horses was highly reliable. It involved use of an easily palpable anatomic landmark on the mediodistal aspect of the TMT joint. An established alternative method for injection of the TMT joint may be useful in clinical practice for diagnostic and therapeutic purposes, although a medial approach may increase risk of injury to veterinarians.
Glucose meters: What’s the laboratory reference glucose?
Authors: Carta Mariarosa , Giavarina Davide , Paternoster Andreina , Bonetti Graziella 
Published: March 2019

SummaryBackgroundThe accuracy of glucose meters is evaluated by comparing their results with those from a reference laboratory glucose analyser. The main scientific societies recommend the use of a prompt glycolysis inhibitor such as citrate for an accurate glucose determination. In the present preliminary study, we discuss the bias between capillary and plasma glucose measured concentrations, determined in two Italian clinical laboratories, using tubes containing an NaF and citrate mixture in liquid and granular form.Methods139 volunteers in whom 75 g OGTT was requested were recruited. Basal capillary glucose was determined using Abbott FreeStyle Precision Neo in Brescia (n=63), while clinical laboratory reference P-glucose was determined using tubes containing NaF/K3EDTA and liquid NaF/Na2EDTA/citrate. Basal capillary glucose was determined using a Roche Cobas Accu-Chek Inform II in Vicenza (n=76), while P-glucose was determined using tubes containing NaF/K2Ox and NaF/Na2EDTA/citrate in granulated form. Reference P-glucose was determined with a hexokinase method on Dimension Vista systems. Differences between capillary and reference P-glucose were evaluated according to ADA/ISO 15197:2013 specifications.Results96.82% and 97.37% of capillary determinations were within specifications when liquid and granular citrate mixture tubes were used, respectively. Conversely, only 73.02% and 80.26% of determinations were within criteria using NaF.ConclusionsIt’s important to know what is the laboratory reference glucose in evaluating glucose meters’ accuracy. The evaluation of glucometers’ accuracy with respect to a reference laboratory may be wrong if tubes containing only NaF are used due to in vitro glycolysis. Only tubes containing citrate mixture permit the correct evaluation of glucose meters’ accuracy.