bluuebunny/crossref_metadata_2025_split huggingface dataset:
Higher risk of revision in total knee arthroplasty after high tibial osteotomy: A systematic review and updated meta-analysis
Authors: Chen Xi , Yang Zhen , Zhu Shibai , Wang Yiou , Qian Wenwei 
Published: October 2019

Abstract
Background: High tibial osteotomy is commonly performed in young patients with high activity demand. It is reported up to 30% of HTO need subsequent conversion to TKA. Primary TKA now provides satisfactory long-term outcome in terms of function and survival. Researches have been conducted to compare clinical outcome between primary TKA and TKA after HTO to see if primary should be the prior treatment rather than HTO in some cases. But the results were inconsistent. This study aims to compare the risk of revision and other parameters of total knee arthroplasty after high tibial osteotomy and primary total knee arthroplasty. Methods: Thorough searches and screens of the relevant literature were conducted, after which data were extracted and pooled analysis was performed to compare the clinical outcomes between the two groups.Results: A total of 14 studies with 144,692 cases were included. Pooled analysis showed significantly more revisions and complications and more tibial component loosening and impingement in postoperative X-ray in the HTO-TKA group. Surgical complexity during conversion to total knee arthroplasty was summarised and listed in a table. Conclusion: Total knee arthroplasty after tibial osteotomy is a technically challenging procedure and provide inferior clinical outcomes than primary TKA. Given the success of primary TKA, it may be considered as the first choice in young patients with high activity demand.
The Suppression of a R2R3-type LoMYB gene damaged the anther dehiscence in lily
Authors: Tong Zheng , Li Qiuhua , Khaskheli AllahJurio , Gao Junping , He Junna , Hong Bo 
Published: October 2019

Abstract
Background Lilies are the widely cultivated cut flowers worldwide, while lily anthers carry a large amount of colored pollen dispersed easily to stain petals that makes serious problems for commerical sales. Improving pollen pollution in lily is one of the major goals of lily breeding.Results In this study, we identified a putative R2R3 MYB transcription factor LoMYB from oriental lily ( Lilium sp. ‘Siberia’). LoMYB mainly expressed in anther wall during the late stages of lily anther development. Suppression of LoMYB by virus-induced gene silencing (VIGS) in lily led to a failure of the anthers to dehisce. Induction of LoMYB in DEX:: LoMYB transgenic Arabidopsis by spraying dexamethasone (DEX), caused the rosette leaves turning yellow and the inflorescences becoming procumbent and infertile. And the induced genes in Arabidopsis include developmental programmed cell death (PCD) and secondary wall biosynthesis (SWB) related genes. These results suggested that MYB regulated anther dehisce possibly through promoting the PCD and SWB processes in cells.Conclusions Our results demonstrated the indispensable role of LoMYB in lily anther dehisce, discovered LoMYB participated in PCD and SWB regulatory processes, and provide a possibility of using LoMYB silencing to produce anther-indehicent lilies.
Effect of inspiratory muscle loaded exercise training on peak oxygen uptake and ventilatory response during incremental exercise under normoxia and hypoxia
Authors: Ogawa Takeshi , Nagao Maiko , Fujii Naoto , NIshiyasu Takeshi 
Published: October 2019

Abstract
Background: Although numerous studies have reported the effect of inspiratory muscle training by attempting to improve exercise performance, the outcome of whether exercise performance is improved by inspiratory muscle training is controversial. Therefore, this study investigated the influence of inspiratory muscle-loaded exercise training (IMLET) on VO2peak, respiratory responses, and exercise performance under N and H exercise conditions. We hypothesized that IMLET enhances respiratory muscle strength and improves respiratory response, thereby VO2peak and work capacity under H condition. Methods: Sixteen university track runners (13 men and 3 women) were randomly assigned to the IMLET (n=8) or exercise training (ET) group (n=8). All subjects underwent 4 weeks of 20-min 60%VO2peak cycling exercise training, thrice per week. IMLET loaded 50% of maximal inspiratory pressure (PImax) during exercise. At pre- and post-training periods, subjects performed exhaustive incremental cycling under normoxic (N; 20.9 ± 0%) and hypoxic (H; 15.0 ± 0.1%) conditions. Results: Although PImax and maximal expiratory pressure significantly increased after training in both groups, the extent of PImax increase was significantly higher in the IMLET group (44.5 ± 27.2% vs. 14.9 ± 11.8%; P<0.05). In both groups, VO2peak and maximal work load (Wmax) similarly increased both under N and H conditions after training (P<0.05). Further, the extent of Wmax decrease under H condition was smaller only in the IMLET group at post-training test than at pre-training (from -14.7 ± 2.2% to -12.5 ± 1.7%; P<0.05). Maximal minute ventilation in both N and H conditions increased after training than in the pre-training period. Conclusions: Our IMLET enhanced respiratory muscle strength, and the effect of hypoxia on work capacity was attenuated with enhancing the respiratory response.
Long-term effects of group exercise intervention on maximal step-up height in female primary care patients with cardio-metabolic risk factors
Authors: Nyberg Lillemor Amanda , Sundberg Carl Johan , Wändell Per , Kowalski Jan , Hellenius Mai-Lis 
Published: October 2019

Abstract
Background: Low physical performance is a predictor of morbidity and mortality. This study looks at long-term effects of an exercise intervention on maximal step-up height (MSH) in individuals with low physical function. Furthermore, we studied correlates to changes in MSH. Methods: Female patients (n = 101), mean (SD) age of 52 (11) years, were recruited for a 3-month group exercise intervention including 2-3 sessions/week of mixed aerobic fitness and strength training. MSH, weight, body mass index (BMI), waist circumference, maximal oxygen consumption (VO 2 -max), self-reported health (SF-36) and physical activity (PA) were measured at baseline (T0), after 3 months (T1) and after 14-30 (mean 22) months (T2). Relationships between changes in MSH (cm) and age, baseline MSH, time to follow-up, changes in anthropometric measurements, VO 2 -max, SF-36 and PA were studied with regression analyses. Results: MSH, significantly, increased from T0 to T1, 27.2 (5.7) to 29.0 (5.5) cm and decreased to 25.2 (5.5) cm at T2. Time to follow-up (B = -0.42, p < 0.001) and change in BMI (B = -0.29, p = 0.012) correlated significantly to changes in MSH. Waist circumference, VO 2 -max, PF and exercise/physical activity levels were significantly improved at T2, while BMI did not change. In a univariate logistic regression model, maintenance of MSH correlated to the extent of mixed training (OR 3.33, 95% CI 1.25-8.89). In a multivariate logistic regression model adjusted for important factors the correlation was not significant. However, MSH was significantly higher in individuals participating in 2-3 session per week compared to one session. Conclusions: A 3-month group exercise intervention increased MSH, improved fitness, decreased risk in female patients with elevated cardio-metabolic risk. After an average of 22 months MSH was reduced while positive effects remained for waist circumference, VO 2 -max, physical function and physical activity. However, regular group exercise 2-3 times per week with mixed aerobic fitness and strength training was associated with maintenance of MSH in a subgroup of patients. We suggest that such an intervention including regular support from healthcare professionals is a successful approach for maintaining improved leg-muscle strength among primary care patients.
Prevention of dental caries using low concentration fluoride gel in custom tray in head and neck cancer patients undergoing radiotherapy: a preliminary study
Authors: Soutome Sakiko , Yanamoto Souichi , Funahara Madoka , Kawashita Yumiko , Yoshimatsu Masako , Murata Maho , Umeda Masahiro 
Published: October 2019

Abstract
Background: To prevent radiation-related dental caries, some studies reported topical application of 1.0 – 2.0% fluoride gel in custom tray for 4-5 minutes every day. However, in Japan, the concentration of fluoride used by patients is limited to less than 0.15 %. The efficacy of low concentration of fluoride gel in custom tray in preventing radiation-related dental caries has not been investigated. Methods: This is a preliminary study. Thirteen patients with head and neck cancer who underwent radiotherapy were enrolled in this study. They wore custom tray containing 0.145% sodium fluoride gel during sleep every night and were examined for newly developed dental caries a year later. Results: No new dental caries were found in the 13 patients one year after radiotherapy. Conclusions: Low concentration fluoride gel in custom tray during sleep could prevent radiation-related dental caries. We are planning to conduct a multicenter phase III randomized controlled trial to examine the efficacy of this method in preventing radiation-related dental caries. Trial registration: University Hospital Medical Information Network Clinical Trials Registry (UMIN-CTR), UMIN000025196. Registered 9 December 2016, https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000028984
Prevalence and associated risk factors of human intestinal parasitic infections: a population-based study in the southeast of Kerman province, southeastern Iran
Authors: Afshar Mohammad Javad Abbaszadeh , Mehni Maryam Barkhori , Rezaeian Mostafa , Mohebali Mehdi , Baigi Vali , Amiri Somayeh , Amirshekari Mohammad Bagher , Hamidinia Ruhollah , Samimi Mohammad 
Published: October 2019

Abstract
Background Determination of the prevalence and distribution pattern of intestinal parasites is a fundamental step to set up an effective control program to improve the health status. This study aimed to determine the prevalence of intestinal parasitic infections and associated risk factors among inhabitants of Rudbar-e Jonub county, southeast of Kerman province, southeastern Iran.Methods In this cross-sectional study, 861 stool specimens were collected from inhabitants of Rudbar-e Jonub county through a multistage cluster sampling method in 2018. The collected specimens were examined by parasitological methods including, direct wet-mounting (for the fresh specimens with a watery consistency), formalin-ethyl acetate sedimentation and agar plate culture.Results The prevalence of intestinal parasites was 34.2% (95% CI 30.1 to 38.2). The prevalence of protozoan parasites 32.3% (95% CI 28.4 to 36.5) was significantly higher than helminthic parasites 3.2% (95% CI 2.1 to 4.7). Blastocystis sp. , Entamoeba coli and Giardia lamblia as protozoan parasite and Hymenolepis nana as helminthic parasite were the most common detected intestinal parasites in the study. Entamoeba histolytica/dispar , Iodamoeba bütschlii , Chilomastix mesnili , Entamoeba hartmanni , Ascaris lambercoides , and Enterobius vermicularis were other detected parasites. Multiple logistic regression revealed a significant association of intestinal parasitic infections with source of drinking water and residency status (rural/urban). Multiple infections with 2 or 3 parasitic agents constituted 22.7% of 295 infected cases.Conclusions This study revealed a high prevalence of intestinal protozoan infections among inhabitants of Rudbar-e Jonub county. Intestinal parasites especially protozoans remain a challenging public health problem wherever sanitation and health measures are limited in Iran.
New insight in understanding and exploring optimal biomarkers in stroke: Key diagnostic markers for clinical treatment
Authors: Zhang Peng , Wang Jiafeng , Fan Aiqin , Wang Xiaolan , Jiang Liying , Jiang Lianying , Lu Changlian , Gu Xuefeng 
Published: October 2019

Abstract
Background A stroke is a complex neurological phenomenon where patients may suddenly experience paralysis, loss of vision or impaired speech because of interruption of blood flow. Stroke is the second leading cause of death and a significant reason for long-term disability worldwide. Optimal biomarkers latent in stroke remain relatively unknown, and efforts are ongoing to effectively define the molecular fingerprints of a stroke. In this study, we utilized microarray gene expression data for the in silico discovery of novel key diagnostic markers which can be utilized for clinical treatment of ischemic stroke.Methods and results We performed differential expression analysis and obtained 20 genes which have at least a two-fold change in the expression level. We also performed gene-disease association studies, functional categories enrichment and GO term enrichment analysis, tissue expression analysis, protein-protein interaction (PPI) analysis, interaction study of miRNAs that target identified biomarkers. The identified diagnostic markers were validated with the PubMed literature using publication enrichment analysis.Conclusions It was observed that most of the identified genes, including AKAP7, ARG1, S100A2, MMP9, TIMP, and CCR7 are markers for early or post-ischemic stroke.
Expression profile of circulating lncRNAs in patients with atrial fibrillation
Authors: Ruan Zhong-bao , Gongben Bing-di , Chen Ge-cai , Zhu Li 
Published: October 2019

Abstract
Background: Atrial fibrillation (AF) is one of the most common cardiac arrhythmias. However, the exact pathophysiology and mechanisms about AF remains unclear. Accumulating studies have demonstrated that long non-coding RNAs (lncRNAs) play a vital role in the regulation of almost all biological processes. However, relationships between AF and lncRNAs are still unknown.
Methods: The peripheral blood monocytes of a total of 20 patients with AF and 20 healthy subjects were collected for gene chip technology to detect differentially expressed lncRNAs. Reverse transcription polymerase chain reaction (RT-PCR) was applied for further verification. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to identify the functions of differentially expressed genes and related pathways.
Results: There were 19 lncRNAs differentially expressed (FC ≥ 2, p <0.05), of which 6 were up-regulated and 13 were down-regulated. Two of three upregulated lncRNAs (P=0.014 and 0.006 for HNRNPU-AS1and LINC00861, respectively) and two of three downregulated lncRNAs (P=0.028 and 0.032 for RP11-443B7.3 and CTD-2616J11.14, respectively) were randomly confirmed by RT‐PCR. And showed a significantly different expression with the RNA-seq results. GO analysis showed that differentially expressed genes enriched in differentially expressed transcripts in biological process were mainly involved in metabolic process, catabolic process and biosynthetic process. Differentially expressed transcripts in cellular component were mainly involved in nuclear lumen, organelle lumen and cytoplasm et al. Differentially expressed transcripts in molecular function were mainly involved in protein binding, RNA binding and molecular function et al. KEGG enrichment pathway analysis showed that some of the enrichment pathways associated with differentially expressed lncRNAs include Calcium signaling pathway, NF-kappa B signaling pathway, cytokine-cytokine receptor interaction and Toll-like receptor signaling pathway, etc. HNRNPU-AS1 was the highest positive correlated lncRNA in the networks.
Conclusions: The expression of lncRNA in peripheral blood of AF patients is different from that of normal people. The physiological functions of these differentially expressed lncRNAs may be related to the pathogenesis of AF, which provide experimental basis and new therapeutic target for prognosis and treatment of patients with AF. HNRNPU-AS1 may play a important role in the pathophysiology and mechanisms of AF.
A qualitative study exploring the barriers and facilitators of implementing a cardiovascular disease risk reducing intervention for people with severe mental illness into primary care contexts across England: the ‘PRIMROSE’ trial.
Authors: Hassan Suzan , Heinkel Samira , Burton Alexandra , Blackburn Ruth , McCloud Tayla , Ross Jamie , Osborn David , Walters Kate 
Published: October 2019

Abstract
Background: People with severe mental illness (SMI) are at greater risk of earlier mortality due to physical health problems including cardiovascular disease (CVD). There is limited work exploring whether physical health interventions for people with SMI can be embedded and/or adopted within specific healthcare settings. This information is necessary to optimise the development of services and interventions within healthcare settings. This study explores the barriers and facilitators of implementing a nurse-delivered intervention (‘PRIMROSE’) designed to reduce CVD risk in people with SMI in primary care, using Normalisation Process Theory (NPT).Methods: Semi-structured interviews were conducted between April-December 2016 with patients with SMI at risk of CVD who received the PRIMROSE intervention, and practice nurses and healthcare assistants who delivered it in primary care in England. Interviews were audio recorded, transcribed and analysed using thematic analysis. Emergent themes were then mapped on to constructs of NPT. Results: 15 patients and 15 staff participated. The implementation of PRIMROSE was affected by: 1) Coherence, where both staff and patients expressed an understanding of the purpose and value of the intervention, 2) Cognitive participation, including mental health stigma, staff confidence levels, staff knowledge and staff perceptions of the compatibility of the intervention to primary care contexts, 3) Collective action, including lack of patient engagement despite flexible appointment scheduling. Limited time and resources hindered implementation. Positive relationships between staff and patients facilitated implementation, and access to ‘in-house’ staff support was considered important. Staff skills, knowledge and training facilitated implementation. However, perceptions of the applicability of the intervention to real-world contexts and accessibility of resources sometimes prevented collective action. 4) Reflexive monitoring, where the staff commonly appraised the intervention by reporting its value and identifying ways of improving it. Conclusions: Future interventions for physical health in people with SMI could consider the following items to improve implementation: 1) training for practitioners covering interpersonal skills, mental and physical health, in order to overcome stigma, increase knowledge, confidence and facilitate positive relationships with patients and 2) enabling access to resources including specialist services, additional staff and time.
The Joint External Evaluation process in Cameroon: Assessing the country’s capacity for health security
Authors: NDOUNGUE Viviane FOSSOUO , Molium Mohamed Moctar Mouiche , Tiwoda Christie , Gnigninanjouena Oumarou , Sadeuh-Mba Serge Alain , Wango Roland Kimbi , Ngapana Arouna Njayou 
Published: October 2019

Abstract
Objectives The objective of this study was to present the JEE process in Cameroon as well as Cameroon’s capacities to manage public health threats in accordance with the IHR 2005. Cameroon JEE process and data for the 48 indicators within the 19 technical areas was examined.Results Cameroon's overall score was 1.98 ± 0.93 and 48/34 indicators (70.38%) had scores less than 2 on a 1 to 5 scale. In the “Detect” category the average score was 2.7. Technical areas with the lowest average scores were Reporting and National laboratory system. The weakest indicators in the “Prevent” category were antimicrobial resistance, biosafety and biosecurity, and National legislation, policy and financing. Emergency Response Operations, Preparedness, Medical Countermeasures and Personnel Deployment have the lowest scores in the “Respond” category. Chemical Events and Points of Entry have the lowest score in “Other IHR-related hazards and Point of Entry”. Scores attributed during the country self-assessment were 73% similar to those of the JEE process.
Application of MALDI-TOF MS to rapid identification of anaerobic bacteria: a meta-analysis
Authors: Li Ying , Shan Mingzhu , Zhu Zuobin , Mao Xuhua , Yan Mingju , Chen Ying , Zhu Qiuju , Li Hongchun , Gu Bing 
Published: October 2019

Abstract
BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification.
OBJECTIVE: To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes.
METHODS: Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P -value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0.
RESULTS : The identification accuracy of MALDI-TOF MS was 84% for species (I 2 = 98.0%, P < 0.1), and 92% for genus (I 2 = 96.6%, P < 0.1). Thereinto, the identification accuracy of Bacteroides was the highest at 96% with a 95% CI of 95-97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs . 86% for MALDI biotyper system.
CONCLUSION: Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria.
Key words : MALDI-TOF MS , anaerobec, bacteria identification