bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunosensor for Detection of the Textile Dye Disperse Orange 1 Based on Non‐conventional Competitive Assay
Authors: Venturini Uliana Carolina , Yamanaka Hideko 
Published: August 2019

AbstractTextile dyes appear as an important class of compounds that has become a matter of public concern and a serious challenge for scientists and environmentalists due to their large‐scale production and extensive application. In this work, a non‐conventional competitive‐type amperometric immunosensor was successfully developed for detection of the textile dye Disperse Orange 1 (DO1). The DO1 was magnetically captured and separated from the sample solution using magnetic particles (MP) functionalized with the antibody anti‐DO1 and with HRP and gold electrodes were modified with the conjugate DO1‐BSA. Molecules of DO1 immobilized on the electrode surface and DO1 captured by MP compete for antibody binding sites. As a result, the amperometric signal decreases with increasing target DO1 concentration at the capture step, because this decreases, the attachment between the HRP coated MP and the electrode. This strategy allowed us to determine DO1 at the low detection limit of 0.87 ng mL−1 with great specificity. Also, there were good recoveries for detection of the textile dye in river water samples without the need of sample pre‐treatment. The competitive amperometric immunosensor shows applicability for the determination of small molecules that cannot be determined by conventional competitive or sandwich immunosensors.
Design and Development of Ultrafast Sinapic Acid Sensor Based on Electrochemically Nanotuned Gold Nanoparticles and Solvothermally Reduced Graphene Oxide
Authors: Kumar Ashutosh , Purohit Buddhadev , Mahato Kuldeep , Roy Sharmili , Srivastava Ananya , Chandra Pranjal 
Published: August 2019

AbstractWe report for the first time sinapic acid (SA) sensing based on nanocomposite comprising electrochemically tuned gold nanoparticles (EAuNPs) and solvothermally reduced graphene oxide (rGO). The synthesized EAuNPs, rGO, and EAuNPs‐rGO nanocomposite were characterized using X‐ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED), particle size analysis, and Raman spectroscopy. A proof‐of‐concept electrochemical sensor for SA was developed based on synthesized EAuNPs‐rGO nanocomposite, which was characterized by electrochemical techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The developed sensor detected SA with a linear dynamic range (LDR) between 20 μM and 200 μM and detection limit (DL) of 33.43 (±0.21) nM (RSD<3.32 %). To show the useful purpose of the sensor probe in clinical applications, SA was detected in human urine samples, which showed the percentage recovery between 82.6 % and 92.8 %. Interferences due to various molecules such as L‐cystine, glycine, alanine, serum albumin, uric acid, citric acid, ascorbic acid, and urea were tested. Long‐term stability of the sensor probe was examined, which was found to be stable up to 6 weeks. The sensor fabricated using EAuNPs‐rGO nanocomposite has many attractive features such as; simplicity, rapidity, and label‐free detection; hence, it could be a method of choice for SA detection in various matrices.
Experiment on morphological and hydraulic adjustments of step‐pool unit to flow increase
Authors: Zhang Chendi , Xu Mengzhen , Hassan Marwan A. , Chartrand Shawn M. , Wang Zhaoyin , Ma Zewei 
Published: August 2019

AbstractExceptional flood events with a return period of about 50 years can be destructive to step‐pool channel segments. However, field investigations and flume experiments have not examined the hydraulic and morphological feedbacks of step‐pool morphology during unsteady hydrographs of exceptional flood events. We performed a series of flume experiments with a manually constructed step model, perturbed with three hydrographs that varied in the rate of water supply change. The bed texture, topography, flow regimes, surface flow field and water depth were characterized and measured as the flow rate was increased during the experiments. A distinct pool feature emerged downstream of the manually constructed step when the flow rate exceeded the threshold scaled to the peaks of ordinary flood events in well‐graded mountain streams. The pool feature was modified in several different ways with flow rate increase. The bed surface steadily coarsened, micro‐bedforms developed and became more pronounced, the bed topography became more spatially complex based on analysis using the Hurst exponent, and last, pool depth steadily increased. Pool modification was also linked to the flow regime: the impinging jet regime led to grain size segmentation in the pool while the jump regime contributed to decelerating flow velocity. The steeper rising limb of hydrograph led to a less developed pool feature, with smaller sized micro‐bedforms in the pool bottom to outlet, and higher discharge threshold for distinct coarsening and scouring in the pool. The estimated energy dissipation within the step‐pool unit decreased as a power function from low to high flow, quantified as the ratio hc/HS, where hc is the critical water depth and HS is scour depth. Our results highlight the interaction between morphology, hydraulics, and energy dissipation of step‐pool unit and the crucial role of hydrograph shape on the interaction during flow increase © 2019 John Wiley & Sons, Ltd.
Monoalkyl Tin Nano-Cluster Films Reveal a Low Environmental Impact under Simulated Natural Conditions
Authors: Wu Fan , Harper Bryan J. , Marsh David A. , Saha Sumit , Diulus Trey , Amador Jenn M. , Keszler Douglas A. , Herman Gregory S. , Maddux Bettye L.S. , Harper Stacey L. 
Published: August 2019

Abstract
Recently, monoalkyl oxo-hydroxo tin clusters have emerged as a new class of metal-oxide resist to support the semiconductor industry's transition to extreme ultraviolet (EUV) lithography. Under EUV exposure, these tin-based clusters exhibit higher performance and wider process windows than conventional polymer materials. A promising new monoalkyl precursor, [(BuSn)12O14(OH)6][OH]2 (BuSn), is still in its infancy in terms of film formation. However, understanding potential environmental effects could significantly affect future development as a commercial product. We synthesized and explored the toxicity of nano-BuSn in the alga Chlamydomonas reinhardtii and the crustacean Daphnia magna at exposure concentrations ranging from 0 to 250 mg/L. Nano-BuSn had no effect on C. reinhardtii growth rate irrespective of concentration, whereas high nanoparticle concentrations (≥100 mg/L) increased D. magna immobilization and mortality significantly. To simulate an end-of-life disposal and leachate contamination, BuSn-coated film wafers were incubated in water at various pH values and temperatures for 14 and 90 d to investigate leaching rates and subsequent toxicity of the leachates. Although small quantities of tin (1.1–3.4% of deposited mass) leached from the wafers, it was insufficient to elicit a toxic response regardless of pH, incubation time, or temperature. The low toxicity of the tin-based thin films suggests that they can be an environmentally friendly addition to the material sets useful for semiconductor manufacturing. Environ Toxicol Chem 2019;38:2651–2658. © 2019 SETAC
Characterization of Miscellaneous Effluent Discharges from a Mobile Offshore Drilling Unit to the Marine Environment
Authors: Hughes Sarah A. , Naile Jonathan , Pinza Meg , Ray Collin , Hester Brian , Baum Julia , Gardiner William , Kallestad Waverly , Brzuzy Louis 
Published: August 2019

Abstract
A study was performed to evaluate the potential biological impacts from 8 different miscellaneous discharges from an oil and gas mobile offshore drilling unit (MODU) including deck drainage, desalination unit waste, boiler blowdown, fire control system test water, noncontact cooling water, and bilge water. Samples were evaluated for toxicity using a rapid (&lt;1 h) initial screening test (echinoderm [Dendraster excentricus] fertilization test), and if toxicity was found, further testing was conducted using 3 chronic whole-effluent toxicity tests. This additional testing included the embryo larval development 72-h echinoderm (D. excentricus); 7-d mysid (Americamysis bahia) survival, growth, and fecundity invertebrate test; and 7-d topsmelt (Atherinops affinis) survival and growth fish test. Toxicity identification evaluations were performed on 3 discharges that consistently elicited a toxic response during whole-effluent toxicity testing. To place the results of the toxicity testing into the context of environmental risk, the spatial extent of potential biological effects was investigated using the CORMIX mixing zone model. The output of the modeling indicated that discharge of selected effluents did not result in concentrations, or duration of exposure, that would elicit toxic effects to organisms living in the surrounding environment. The present study provides a comprehensive data set that was used to characterize potential toxicity and environmental risk of MODU “miscellaneous discharges” which could help inform future risk assessments of these discharges. Environ Toxicol Chem 2019;38:2811–2823. © 2019 SETAC
Petrographic and geochemical study of the stone warrior stelae from central Iberia: Linking the geological record and archaeological heritage
Authors: Merino Martínez E. , Andonaegui P. , Chapa T. , Pereira Sieso J. 
Published: August 2019

AbstractSix warrior stelae from central Iberia (Toledo, Spain) were studied using different geological techniques to discriminate their potential source materials and evaluate the displacement suffered from the quarry sites to their final location. The similar petrographic and geochemical features of the stelae, when compared with other lithological equivalents cropping out in the surrounding area, suggest that most raw materials were quarried less than 10 km from the finds. These materials mainly belong to a set of metasedimentary rocks from the Schist–Graywacke Complex and other Ordovician brownish sandstones, mostly found in the vicinity of the stelae sites. In contrast, the Talavera de la Reina stele, previously used as a statue‐menhir, was engraved on a leucogranite, geochemically similar to some plutonic units in the Navamorcuende area, in the southernmost region of the Spanish Central System batholith. The fact that all stelae and the proposed geological sources were located close to ancient tracks suggests that the raw materials were quarried in connection with these natural routes. The stelae may, therefore, have been landmarks or reference points that signaled transit passages used for trade or grazing, in the framework of continuous territorial expansion during the Late Bronze Age and Early Iron Age.
Swallowing‐related outcomes associated with late lower cranial neuropathy in long‐term oropharyngeal cancer survivors: cross‐sectional survey analysis
Authors: Aggarwal Puja , Zaveri Jhankruti S. , Goepfert Ryan P. , Shi Qiuling , Du Xianglin L. , Swartz Michael , Lai Stephen Y. , Fuller C. David , Lewin Jan S. , Piller Linda B. , Hutcheson Katherine A. 
Published: August 2019

AbstractBackgroundThe purpose of this study was to quantify the association of late lower cranial neuropathy (late LCNP) with swallowing‐related quality of life (QOL) and functional status among long‐term oropharyngeal cancer (OPC) survivors.MethodsEight hundred eighty‐nine OPC survivors (median survival time: 7 years) who received primary treatment at a single institution between January 2000 and December 2013 completed a cross‐sectional survey (56% response rate) that included the MD Anderson Dysphagia Inventory (MDADI) and self‐report of functional status. Late LCNP events ≥3 months after cancer therapy were abstracted from medical records. Multivariate models regressed MDADI scores on late LCNP status adjusting for clinical covariates.ResultsOverall, 4.0% (n = 36) of respondents developed late LCNP with median time to onset of 5.25 years post‐treatment. LCNP cases reported significantly worse mean composite MDADI (LCNP: 68.0 vs no LCNP: 80.2; P < .001). Late LCNP independently associated with worse mean composite MDADI (β = −6.7, P = .02; 95% confidence interval [CI], −12.0 to −1.3) as well as all MDADI domains after multivariate adjustment. LCNP cases were more likely to have a feeding tube at time of survey (odds ratio [OR] = 20.5; 95% CI, 8.6‐48.9), history of aspiration pneumonia (OR = 23.5; 95% CI, 9.6‐57.6), and tracheostomy (OR = 26.9; 95% CI, 6.0‐121.7).ConclusionsIn this large survey study, OPC survivors with late LCNP reported significantly poorer swallowing‐related QOL and had significantly higher likelihood of poor functional status. Further efforts are necessary to optimize swallowing outcomes to improve QOL in this subgroup of survivors.
Lean NAFLD: A Distinct Entity Shaped by Differential Metabolic Adaptation
Authors: Chen Fei , Esmaili Saeed , Rogers Geraint B. , Bugianesi Elisabetta , Petta Salvatore , Marchesini Giulio , Bayoumi Ali , Metwally Mayada , Azardaryany Mahmoud Karimi , Coulter Sally , Choo Jocelyn M. , Younes Ramy , Rosso Chiara , Liddle Christopher , Adams Leon A. , Craxì Antonio , George Jacob , Eslam Mohammed 
Published: August 2019

Background and Aims
Nonalcoholic fatty liver disease (NAFLD) affects a quarter of the adult population. A significant subset of patients are lean, but their underlying pathophysiology is not well understood.

Approach and Results
We investigated the role of bile acids (BAs) and the gut microbiome in the pathogenesis of lean NAFLD. BA and fibroblast growth factor (FGF) 19 levels (a surrogate for intestinal farnesoid X receptor [FXR] activity), patatin‐like phospholipase domain containing 3 (PNPLA3), and transmembrane 6 superfamily member 2 (TM6SF2) variants, and gut microbiota profiles in lean and nonlean NAFLD were investigated in a cohort of Caucasian patients with biopsy‐proven NAFLD (n = 538), lean healthy controls (n = 30), and experimental murine models. Patients with lean NAFLD had a more favorable metabolic and histological profile compared with those with nonlean NAFLD (P < 0.05 for all). BA levels were significantly higher in NAFLD with advanced compared with earlier stages of liver fibrosis. Patients with lean NAFLD had higher serum secondary BA and FGF19 levels and reduced 7‐alpha‐hydroxy‐4‐cholesten‐3‐one (C4) levels (P < 0.05 for all). These differences were more profound in early compared with advanced stages of fibrosis (P < 0.05 for both). Lean patients demonstrated an altered gut microbiota profile. Similar findings were demonstrated in lean and nonlean murine models of NAFLD. Treating mice with an apical sodium‐dependent BA transporter inhibitor (SC‐435) resulted in marked increases in fgf15, a shift in the BA and microbiota profiles, and improved steatohepatitis in the lean model.

Conclusions
Differences in metabolic adaptation between patients with lean and nonlean NAFLD, at least in part, explain the pathophysiology and provide options for therapy.
Evidence for similarity in symptoms and mechanism: The extra‐pulmonary symptoms of severe asthma and the polysymptomatic presentation of fibromyalgia
Authors: Hyland Michael E. , Lanario Joseph W. , Wei Yinghui , Jones Rupert C. , Masoli Matthew 
Published: August 2019

AbstractBackgroundAsthma is a disease of the lung and a systemic disease. Functional disorders are associated with multiple systemic abnormalities that have been explained by complexity models. The aim was to test the similarity in type and aetiology between the extra‐pulmonary symptoms of severe asthma and the symptoms of fibromyalgia.MethodsOne Hundred patients recruited from a specialist severe asthma clinic and 1751 people reporting different functional disorder diagnoses recruited via the internet completed the same 60‐item questionnaire. Symptom patterns were compared between groups using a new measure, the symptom pattern similarity index where 0 = no relationship, 1 = identical patterns between groups.ResultsSevere asthma patients report numerous extra‐pulmonary symptoms. The similarity index between the symptom pattern of the asthma patients with other groups was irritable bowel syndrome = 0.54, chronic fatigue syndrome = 0.69, and fibromyalgia = 0.75. The index between fibromyalgia and asthma patients with the most and least frequent extra‐pulmonary symptoms was 0.81 and 0.55 respectively.ConclusionsPatients with severe asthma have numerous extra‐pulmonary symptoms similar in type and pattern to the symptoms of fibromyalgia. The similarity of the symptom pattern between asthma and fibromyalgia increases as the number of extra‐pulmonary symptoms increases as predicted by network theory and previously shown to be the case with other functional disorders. These findings support the hypothesis that functional disorders and extra‐pulmonary asthma symptoms have a common complexity or network aetiology. Evidence based behavioural interventions for fibromyalgia may be helpful for patients with severe asthma reporting extra‐pulmonary symptoms.
Bacteriophage delivering hydrogels reduce biofilm formation in vitro and infection in vivo
Authors: Wroe James A. , Johnson Christopher T. , García Andrés J. 
Published: August 2019

AbstractImplanted orthopedic devices become infected more frequently than any other implanted surgical device. These infections can be extremely costly and result in significant patient morbidity. Current treatment options typically involve the long term, systemic administration of a combination of antibiotics, often followed by implant removal. Here we engineered an injectable hydrogel capable of encapsulating Pseudomonas aeruginosa bacteriophage and delivering active phage to the site of bone infections. Bacteriophage retain their bacteriolytic activity after encapsulation and release from the hydrogel, and their rate of release from the hydrogel can be controlled by gel formulation. Bacteriophage‐encapsulating hydrogels effectively kill their host bacteria in both planktonic and biofilm phenotypes in vitro without influencing the metabolic activity of human mesenchymal stromal cells. Bacteriophage‐encapsulating hydrogels were used to treat murine radial segmental defects infected with P. aeruginosa. The hydrogels achieved a 4.7‐fold reduction in live P. aeruginosa counts at the infection site compared to bacteriophage‐free hydrogels at 7 days postimplantation. These results support the development of bacteriophage‐delivering hydrogels to treat local bone infections.
Green synthesis of bio‐ethyl acetate over Egyptian natural red clay as a highly active, selective, and eco‐friendly catalyst
Authors: Said Abd El‐Aziz A. , Goda Mohamed N. 
Published: August 2019

AbstractThe present work focused on the utilization of Egyptian red clay (ERC) without any treatment as a highly efficient, selective, and eco‐friendly catalyst for synthesis of bio‐ethyl acetate in the gas phase. The best conditions used in conventional fixed bed reactor were 130 cm3/min total flow rate of air with 1.6 and 2.1 vol% bio‐ethanol and bio‐acetic acid, respectively, 1 g clay catalyst calcined at 300 and 225°C reaction temperature. Under these conditions, the conversions to ester achieved were about 80 and 98% after 5 and 30 min, respectively, from the initial admission of the reactants into the reactor. The amount and type of surface acidity were identified by isopropanol reaction and chemisorption of basic probes. The results demonstrated that the majority of intermediate strength of BrØnsted acid sites are the key role for the dehydration reaction toward ester formation. The virgin and annealed clay solids were characterized using X‐ray fluorescence (XRF), thermogravimetry (TG) and differential thermal analysis (DTA), Fourier transform infrared (FTIR), X‐ray diffraction (XRD), and N2 sorption.
Development and validation of T‐ARMS‐PCR to detect CYP2C19*17 allele
Authors: Jin Chenxi , Li Zhikun , Zheng Xiaodi , Shen Kailin , Chao Jiashuo , Dong Yifei , Huang Qin , Yin Qiulin , Deng Yan , Zhu Weifeng 
Published: August 2019

AbstractBackgroundCYP2C19*17 (rs12248560) is a functional single nucleotide polymorphism (SNP) in the CYP2C19 gene. It has been shown that CYP2C19*17 is associated with the clinical outcome of some drugs metabolized by CYP2C19 and a decreased risk of some diseases. The aim of this study was to develop a reliable and simple method to detect this polymorphism.MethodsTetra‐primer amplification refractory mutation system‐polymerase chain reaction (T‐ARMS‐PCR) was used to detect the CYP2C19*17 polymorphism. A total of 93 samples were screened by this method, and the results of T‐ARMS‐PCR were validated by DNA sequencing.ResultsThere were 91 samples with the CC genotype (97.8%) and two samples with the CT genotype (2.2%). The frequency of the C allele was 98.9%, and the frequency of the T allele was 1.1%. The DNA sequencing results were completely concordant with the T‐ARMS‐PCR results.ConclusionT‐ARMS‐PCR can detect the CYP2C19*17 polymorphism with high accuracy, low costs, and a simple process.
Assessment of hypercoagulability using thromboelastography predicts advanced status in renal cell carcinoma
Authors: Wang Xun , Shi An , Huang Jiwei , Chen Yonghui , Xue Wei , Zhang Jin 
Published: August 2019

AbstractBackgroundThromboelastography (TEG) has been established as a sensitive method to assess the whole coagulation process. The aim of the study was to evaluate the diagnosis significance of TEG on hypercoagulability in patients suffering renal mass.MethodsA total of 478 patients were diagnosed with renal tumor by histolopathologic examination and were assigned to three groups. Group A: 79 patients with benign renal tumor; Group B: 317 patients with renal cell carcinoma (RCC, Fuhrman grades I and II); Group C: 82 patients with high‐risk RCC (Fuhrman grades III and IV). Subgroup analysis was performed in malignant renal tumor patients according to the TMN classification. The clinical data, whole blood TEG, and conventional coagulation tests were reviewed.ResultsThere was no statistically significant difference between subgroups in respect to conventional coagulation tests. Hypercoagulablity was marked in Group C according to the TEG parameters. The elevated platelets and fibrinogen is linked with hypercoagulability in renal tumor. The positive correlation was between fibrinogen and MA value (r = .663, P < .05). The pathologic tumor stages were also associated with the TEG parameters.ConclusionPatients suffering advanced RCC are hypercoagulable which can be identified by TEG. MA value could be potential diagnosis indicators for detecting high‐grade RCC.