bluuebunny/crossref_metadata_2025_split huggingface dataset:
Social Media and Change in Psychological Distress Over Time: The Role of Social Causation
Authors: Hampton Keith N 
Published: May 2019

AbstractThis article tests the relationship between information and communication technologies (ICT), such as the Internet, cell phones, and social media, and change over time in psychological distress (PD) and risk of serious psychological distress (SPD) associated with depression and anxiety disorders. Using a longitudinal panel design, survey data from a representative sample of American adults, findings revealed that home Internet and social network site (SNS) use are associated with decreased PD over time. Having extended family who are also Internet users further decreases PD. PD increased or decreased in relation to change in the PD of extended family who also use SNSs. For most people, ICT substantively reduce PD; in rare cases, an extreme spike in PD of extended family also on SNSs, there was a trivial increase to the risk of SPD. PD did not change when extended family not on social media experienced a change in their PD.
Head Lice Recognize and Prefer Head Odor Over Foot and Forearms Odors
Authors: Galassi Federico , Gonzalez-Audino Paola , Picollo María I 
Published: May 2019

Abstract
Human head lice, Pediculus humanus capitis De Geer (Phthiraptera: Pediculidae), are hematophagous parasites that infest human heads. They are extreme host specialists suggesting a strict selection behavior towards the human head by the parasites. Despite the public health relevance of P. humanus capitis, the role of chemical clues to select the human head is not well known. In the present study, we attempted to find out whether head lice recognize and select the odor of the head over the odor of other parts of the human body. Our results using a two-choice olfactometer demonstrated that head lice were highly attracted by the volatile compounds of the human odor air, but they did not show preference for the volatiles of head compared to the volatiles of other parts of the body (forearm or foot). Conversely, when head lice were exposed in an experimental arena to the whole human odor of different parts of the body, they showed a preferential response to the whole head odor compared with the whole foot or forearm odors. These results suggest that head lice can at short distance of the host, be oriented towards the head odor of the human host in the selection of the scalp environment.
How to claim what is mine: Negotiating professional roles in inter-organizational projects
Authors: Bos-de Vos Marina , Lieftink Bente M , Lauche Kristina 
Published: May 2019

AbstractProfessional roles within inter-organizational projects have become increasingly diverse and contested, yet little is known about how professionals react to such threats of marginalization. Drawing on empirical data from interviews with architects, a profession in which historically established role boundaries have become particularly blurred, we analyse how professionals negotiate their roles in inter-organizational projects. We identified three types of boundary work—reinstating, bending, and pioneering role boundaries—and illustrate their antecedents and effects for project collaboration. These categories exemplify different responses to the threat of marginalization depending upon professionals’ perceptions of what the specific project called for. Our study provides important insights into boundary work practices emerging in the context of inter-organizational projects and how professionals adjust their claims-making to perceived opportunities, thereby triggering incremental as well as more radical changes in the professional role structures.
Using failed supernovae to constrain the Galactic r-process element production
Authors: Wehmeyer B , Fröhlich C , Côté B , Pignatari M , Thielemann F-K 
Published: May 2019

ABSTRACT
Rapid neutron capture process (r-process) elements have been detected in a large fraction of metal-poor halo stars, with abundances relative to iron (Fe) that vary by over two orders of magnitude. This scatter is reduced to less than a factor of 3 in younger Galactic disc stars. The large scatter of r-process elements in the early Galaxy suggests that the r-process is made by rare events, like compact binary mergers and rare sub-classes of supernovae. Although being rare, neutron star mergers alone have difficulties to explain the observed enhancement of r-process elements in the lowest metallicity stars compared to Fe. The supernovae producing the two neutron stars already provide a substantial Fe abundance where the r-process ejecta from the merger would be injected. In this work we investigate another complementary scenario, where the r-process occurs in neutron star-black hole mergers in addition to neutron star mergers. Neutron star-black hole mergers would eject similar amounts of r-process matter as neutron star mergers, but only the neutron star progenitor would have produced Fe. Furthermore, a reduced efficiency of Fe production from single stars significantly alters the age–metallicity relation, which shifts the onset of r-process production to lower metallicities. We use the high-resolution [(20 pc)3/cell] inhomogeneous chemical evolution tool ‘ICE’ to study the outcomes of these effects. In our simulations, an adequate combination of neutron star mergers and neutron star-black hole mergers qualitatively reproduces the observed r-process abundances in the Galaxy.
Validation of a temperate fourth planet in the K2-133 multiplanet system
Authors: Wells R , Poppenhaeger K , Watson C A 
Published: May 2019

Abstract
We present follow-up observations of the K2-133 multiplanet system. Previously, we announced that K2-133 contained three super-Earths orbiting an M1.5V host star – with tentative evidence of a fourth outer-planet orbiting at the edge of the temperate zone. Here, we report on the validation of the presence of the fourth planet, determining a radius of $1.73_{-0.13}^{+0.14}$ R⊕. The four planets span the radius gap of the exoplanet population, meaning further follow-up would be worthwhile to obtain masses and test theories of the origin of the gap. In particular, the trend of increasing planetary radius with decreasing incident flux in the K2-133 system supports the claim that the gap is caused by photo-evaporation of exoplanet atmospheres. Finally, we note that K2-133 e orbits on the edge of the star's temperate zone, and that our radius measurement allows for the possibility that this is a rocky world. Additional mass measurements are required to confirm or refute this scenario.
Protein interactions in T7 DNA replisome inhibit the bypass of abasic site by DNA polymerase
Authors: Zou Zhenyu , Liang Tingting , Xu Zhongyan , Xie Jiayu , Zhang Shuming , Chen Weina , Wan Siqi , Ling Yihui , Zhang Huidong 
Published: May 2019

AbstractAbasic site as a common DNA lesion blocks DNA replication and is highly mutagenic. Protein interactions in T7 DNA replisome facilitate DNA replication and translesion DNA synthesis. However, bypass of an abasic site by T7 DNA replisome has never been investigated. In this work, we used T7 DNA replisome and T7 DNA polymerase alone as two models to study DNA replication on encountering an abasic site. Relative to unmodified DNA, abasic site strongly inhibited primer extension and completely blocked strand-displacement DNA synthesis, due to the decreased fraction of enzyme–DNA productive complex and the reduced average extension rates. Moreover, abasic site at DNA fork inhibited the binding of DNA polymerase or helicase onto fork and the binding between polymerase and helicase at fork. Notably and unexpectedly, we found DNA polymerase alone bypassed an abasic site on primer/template (P/T) substrate more efficiently than did polymerase and helicase complex bypass it at fork. The presence of gp2.5 further inhibited the abasic site bypass at DNA fork. Kinetic analysis showed that this inhibition at fork relative to that on P/T was due to the decreased fraction of productive complex instead of the average extension rates. Therefore, we found that protein interactions in T7 DNA replisome inhibited the bypass of DNA lesion, different from all the traditional concept that protein interactions or accessory proteins always promote DNA replication and DNA damage bypass, providing new insights in translesion DNA synthesis performed by DNA replisome.
sRNAbench and sRNAtoolbox 2019: intuitive fast small RNA profiling and differential expression
Authors: Aparicio-Puerta Ernesto , Lebrón Ricardo , Rueda Antonio , Gómez-Martín Cristina , Giannoukakos Stavros , Jaspez David , Medina José María , Zubkovic Andreja , Jurak Igor , Fromm Bastian , Marchal Juan Antonio , Oliver José , Hackenberg Michael 
Published: May 2019

Abstract
Since the original publication of sRNAtoolbox in 2015, small RNA research experienced notable advances in different directions. New protocols for small RNA sequencing have become available to address important issues such as adapter ligation bias, PCR amplification artefacts or to include internal controls such as spike-in sequences. New microRNA reference databases were developed with different foci, either prioritizing accuracy (low number of false positives) or completeness (low number of false negatives). Additionally, other small RNA molecules as well as microRNA sequence and length variants (isomiRs) have continued to gain importance. Finally, the number of microRNA sequencing studies deposited in GEO nearly triplicated from 2014 (280) to 2018 (764). These developments imply that fast and easy-to-use tools for expression profiling and subsequent downstream analysis of miRNA-seq data are essential to many researchers. Key features in this sRNAtoolbox release include addition of all major RNA library preparation protocols to sRNAbench and improvements in sRNAde, a tool that summarizes several aspects of small RNA sequencing studies including the detection of consensus differential expression. A special emphasis was put on the user-friendliness of the tools, for instance sRNAbench now supports parallel launching of several jobs to improve reproducibility and user time efficiency.
Thermodynamically stable and genetically unstable G-quadruplexes are depleted in genomes across species
Authors: Puig Lombardi Emilia , Holmes Allyson , Verga Daniela , Teulade-Fichou Marie-Paule , Nicolas Alain , Londoño-Vallejo Arturo 
Published: May 2019

AbstractG-quadruplexes play various roles in multiple biological processes, which can be positive when a G4 is involved in the regulation of gene expression or detrimental when the folding of a stable G4 impairs DNA replication promoting genome instability. This duality interrogates the significance of their presence within genomes. To address the potential biased evolution of G4 motifs, we analyzed their occurrence, features and polymorphisms in a large spectrum of species. We found extreme bias of the short-looped G4 motifs, which are the most thermodynamically stable in vitro and thus carry the highest folding potential in vivo. In the human genome, there is an over-representation of single-nucleotide-loop G4 motifs (G4-L1), which are highly conserved among humans and show a striking excess of the thermodynamically least stable G4-L1A (G3AG3AG3AG3) sequences. Functional assays in yeast showed that G4-L1A caused the lowest levels of both spontaneous and G4-ligand-induced instability. Analyses across 600 species revealed the depletion of the most stable G4-L1C/T quadruplexes in most genomes in favor of G4-L1A in vertebrates or G4-L1G in other eukaryotes. We discuss how these trends might be the result of species-specific mutagenic processes associated to a negative selection against the most stable motifs, thus neutralizing their detrimental effects on genome stability while preserving positive G4-associated biological roles.
A survey of molecular cores in M 17 SWex
Authors: Shimoikura Tomomi , Dobashi Kazuhito , Hirose Asha , Nakamura Fumitaka , Shimajiri Yoshito , Sugitani Koji 
Published: May 2019

Abstract
A survey of molecular cores covering the infrared dark cloud known as the M 17 southwest extension (M 17 SWex) has been carried out with the 45 m Nobeyama Radio Telescope. Based on the N2H+ (J = 1–0) data obtained, we have identified 46 individual cores whose masses are in the range from 43 to $3026\, {M}_{\odot }$. We examined the relationship between the physical parameters of the cores and those of young stellar objects (YSOs) associated with the cores found in the literature. The comparison of the virial mass and the core mass indicates that most of the cores can be gravitationally stable if we assume a large external pressure. Among the 46 cores, we found four massive cores with YSOs. They have large masses of $\gtrsim 1000\, M_{\odot }$ and line widths of $\gtrsim 2.5\:$km s−1 which are similar to those of clumps forming high-mass stars. However, previous studies have shown that there is no active massive star formation in this region. Recent measurements of near-infrared polarization imply that the magnetic field around M 17 SWex is likely to be strong enough to support the cores against self-gravity. We therefore suggest that the magnetic field may prevent the cores from collapsing, causing the low level of massive star formation in M 17 SWex.
A Functional Alternative Oxidase Modulates Plant Salt Tolerance in Physcomitrella patens
Authors: Wu Guochun , Li Sha , Li Xiaochuan , Liu Yunhong , Zhao Shuangshuang , Liu Baohui , Zhou Huapeng , Lin Honghui 
Published: May 2019

Abstract
Alternative oxidase (AOX) has been reported to be involved in mitochondrial function and redox homeostasis, thus playing an essential role in plant growth as well as stress responses. However, its biological functions in nonseed plants have not been well characterized. Here, we report that AOX participates in plant salt tolerance regulation in moss Physcomitrella patens (P. patens). AOX is highly conserved and localizes to mitochondria in P. patens. We observed that PpAOX rescued the impaired cyanide (CN)-resistant alternative (Alt) respiratory pathway in Arabidopsis thaliana (Arabidopsis) aox1a mutant. PpAOX transcription and Alt respiration were induced upon salt stress in P. patens. Using homologous recombination, we generated PpAOX-overexpressing lines (PpAOX OX). PpAOX OX plants exhibited higher Alt respiration and lower total reactive oxygen species accumulation under salt stress condition. Strikingly, we observed that PpAOX OX plants displayed decreased salt tolerance. Overexpression of PpAOX disturbed redox homeostasis in chloroplasts. Meanwhile, chloroplast structure was adversely affected in PpAOX OX plants in contrast to wild-type (WT) P. patens. We found that photosynthetic activity in PpAOX OX plants was also lower compared with that in WT. Together, our work revealed that AOX participates in plant salt tolerance in P. patens and there is a functional link between mitochondria and chloroplast under challenging conditions.
Reading for Evidence of Faith in Herbert’s Poems
Authors: Lyne Raphael 
Published: May 2019

Abstract
In this essay George Herbert’s representations of inward life are seen in the context of (i) the recommendations for self-examination made by theologians of his own time, and (ii) critical terms derived from modern psychological accounts of ‘Theory of Mind’. Lisa Zunshine’s emphasis on ‘embodied transparency’ and ‘metapresentation’ helps capture the way that Herbert exposes his readers to difficult but salutary self-analysis. This is not just a matter of trapping the unwary: the poems are designed for re-reading, re-voicing, and re-thinking, for considering especially the timing of discoveries about one’s own faith, and the origins of the voices that console and cajole the speakers of the poems. In Herbert’s hands, lyric form and the practice of self-scrutiny both respond dynamically to the pressures that resulted from the religious and cultural environment.
Measuring shared responses across subjects using intersubject correlation
Authors: Nastase Samuel A , Gazzola Valeria , Hasson Uri , Keysers Christian 
Published: May 2019

AbstractOur capacity to jointly represent information about the world underpins our social experience. By leveraging one individual’s brain activity to model another’s, we can measure shared information across brains—even in dynamic, naturalistic scenarios where an explicit response model may be unobtainable. Introducing experimental manipulations allows us to measure, for example, shared responses between speakers and listeners or between perception and recall. In this tutorial, we develop the logic of intersubject correlation (ISC) analysis and discuss the family of neuroscientific questions that stem from this approach. We also extend this logic to spatially distributed response patterns and functional network estimation. We provide a thorough and accessible treatment of methodological considerations specific to ISC analysis and outline best practices.
The TGFβ1 Receptor Antagonist GW788388 Reduces JNK Activation and Protects Against Acetaminophen Hepatotoxicity in Mice
Authors: McMillin Matthew , Grant Stephanie , Frampton Gabriel , Petrescu Anca D , Williams Elaina , Jefferson Brandi , DeMorrow Sharon 
Published: May 2019

AbstractAcute liver failure is a serious consequence of acetaminophen (APAP)-induced hepatotoxic liver injury with high rates of morbidity and mortality. Transforming growth factor beta 1 (TGFβ1) is elevated during liver injury and influences hepatocyte senescence during APAP-induced hepatotoxicity. This study investigated TGFβ1 signaling in the context of inflammation, necrotic cell death, and oxidative stress during APAP-induced liver injury. Male C57Bl/6 mice were injected with 600 mg/kg APAP to generate liver injury in the presence or absence of the TGFβ receptor 1 inhibitor, GW788388, 1 h prior to APAP administration. Acetaminophen-induced liver injury was characterized using histological and biochemical measures. Transforming growth factor beta 1 expression and signal transduction were assessed using immunohistochemistry, Western blotting and ELISA assays. Hepatic necrosis, liver injury, cell proliferation, hepatic inflammation, and oxidative stress were assessed in all mice. Acetaminophen administration significantly induced necrosis and elevated serum transaminases compared with control mice. Transforming growth factor beta 1 staining was observed in and around areas of necrosis with phosphorylation of SMAD3 observed in hepatocytes neighboring necrotic areas in APAP-treated mice. Pretreatment with GW788388 prior to APAP administration in mice reduced hepatocyte cell death and stimulated regeneration. Phosphorylation of SMAD3 was reduced in APAP mice pretreated with GW788388 and this correlated with reduced hepatic cytokine production and oxidative stress. These results support that TGFβ1 signaling plays a significant role in APAP-induced liver injury by influencing necrotic cell death, inflammation, oxidative stress, and hepatocyte regeneration. In conclusion, targeting TGFβ1 or downstream signaling may be a possible therapeutic target for the management of APAP-induced liver injury.
Aneugen Molecular Mechanism Assay: Proof-of-Concept With 27 Reference Chemicals
Authors: Bernacki Derek T , Bryce Steven M , Bemis Jeffrey C , Dertinger Stephen D 
Published: May 2019

Abstract
A tiered bioassay and data analysis scheme is described for elucidating the most common molecular targets responsible for chemical-induced in vitro aneugenicity: tubulin destabilization, tubulin stabilization, and inhibition of mitotic kinase(s). To evaluate this strategy, TK6 cells were first exposed to each of 27 presumed aneugens over a range of concentrations. After 4 and 24 h of treatment, γH2AX, p53, phospho-histone H3 (p-H3), and polyploidization biomarkers were evaluated using the MultiFlow DNA Damage Assay Kit. The assay identified 27 of 27 chemicals as genotoxic, with 25 exhibiting aneugenic signatures, 1 aneugenic and clastogenic, and 1 clastogenic. Subsequently, a newly described follow-up assay was employed to investigate the aneugenic agents’ molecular targets. For these experiments, TK6 cells were exposed to each of 26 chemicals in the presence of 488 Taxol. After 4 h, cells were lysed and the liberated nuclei and mitotic chromosomes were stained with a nucleic acid dye and labeled with fluorescent antibodies against p-H3 and Ki-67. Flow cytometric analyses revealed that alterations to 488 Taxol-associated fluorescence were only observed with tubulin binders—increases in the case of tubulin stabilizers, decreases with destabilizers. Mitotic kinase inhibitors with known Aurora kinase B inhibiting activity were the only aneugens that dramatically decreased the ratio of p-H3-positive to Ki-67-positive nuclei. Unsupervised hierarchical clustering based on 488 Taxol fluorescence and p-H3: Ki-67 ratios clearly distinguished compounds with these disparate molecular mechanisms. Furthermore, a classification algorithm based on an artificial neural network was found to effectively predict molecular target, as leave-one-out cross-validation resulted in 25/26 agreement with a priori expectations. These results are encouraging, as they suggest that an adequate number of training set chemicals, in conjunction with a machine learning algorithm based on 488 Taxol, p-H3, and Ki-67 responses, can reliably elucidate the most commonly encountered aneugenic molecular targets.
Cytomegalovirus distribution and evolution in hominines
Authors: Murthy Sripriya , O’Brien Kathryn , Agbor Anthony , Angedakin Samuel , Arandjelovic Mimi , Ayimisin Emmanuel Ayuk , Bailey Emma , Bergl Richard A , Brazzola Gregory , Dieguez Paula , Eno-Nku Manasseh , Eshuis Henk , Fruth Barbara , Gillespie Thomas R , Ginath Yisa , Gray Maryke , Herbinger Ilka , Jones Sorrel , Kehoe Laura , Kühl Hjalmar , Kujirakwinja Deo , Lee Kevin , Madinda Nadège F , Mitamba Guillain , Muhindo Emmanuel , Nishuli Radar , Ormsby Lucy J , Petrzelkova Klara J , Plumptre Andrew J , Robbins Martha M , Sommer Volker , Ter Heegde Martijn , Todd Angelique , Tokunda Raymond , Wessling Erin , Jarvis Michael A , Leendertz Fabian H , Ehlers Bernhard , Calvignac-Spencer Sébastien 
Published: May 2019

Abstract
Herpesviruses are thought to have evolved in very close association with their hosts. This is notably the case for cytomegaloviruses (CMVs; genus Cytomegalovirus) infecting primates, which exhibit a strong signal of co-divergence with their hosts. Some herpesviruses are however known to have crossed species barriers. Based on a limited sampling of CMV diversity in the hominine (African great ape and human) lineage, we hypothesized that chimpanzees and gorillas might have mutually exchanged CMVs in the past. Here, we performed a comprehensive molecular screening of all 9 African great ape species/subspecies, using 675 fecal samples collected from wild animals. We identified CMVs in eight species/subspecies, notably generating the first CMV sequences from bonobos. We used this extended dataset to test competing hypotheses with various degrees of co-divergence/number of host switches while simultaneously estimating the dates of these events in a Bayesian framework. The model best supported by the data involved the transmission of a gorilla CMV to the panine (chimpanzee and bonobo) lineage and the transmission of a panine CMV to the gorilla lineage prior to the divergence of chimpanzees and bonobos, more than 800,000 years ago. Panine CMVs then co-diverged with their hosts. These results add to a growing body of evidence suggesting that viruses with a double-stranded DNA genome (including other herpesviruses, adenoviruses, and papillomaviruses) often jumped between hominine lineages over the last few million years.