bluuebunny/crossref_metadata_2025_split huggingface dataset:
Des arts à la théorie de l'action: Le travail sociologique de Pierre-Michel Menger
Authors: Fabiani Jean-Louis 
Published: March 2018

Résumé
Cet article tente de montrer l’originalité du travail sociologique de Pierre-Michel Menger à travers l’étude de sa trajectoire théorique. Le point de départ peut être trouvé dans le croisement fécond entre l’analyse des professions et la justification des politiques publiques ; la musique contemporaine est tributaire du financement public alors que la demande publique n’est pas encore advenue. Les acteurs doivent prendre des décisions dans un monde incertain : ce thème constitue le fil conducteur de l’entreprise de P.-M. Menger, particulièrement dans son ouvrage majeur, Le travail créateur. Comment la sociologie doit-elle traiter l’incertitude qui règle les ajustements interindividuels ? Telle est la tâche assignée à une science sociale innovante. P.-M. Menger n’importe pas le raisonnement économique en sociologie, mais il confronte les approches de manière critique en les maintenant en tension, une position qui le distingue des partisans de la théorie du choix rationnel. Son programme de recherche est ambitieux, mais deux questions demeurent : qu’en est-il de l’histoire dans le modelé en construction ? Comment avoir une vision plus claire du concept de talent, lequel est encore souvent une boîte noire ?
Molecular link between glucose and glutamine consumption in cancer cells mediated by CtBP and SIRT4
Authors: Wang Li , Li Jing-jing , Guo Li-yu , Li Peipei , Zhao Zhiqiang , Zhou Haisheng , Di Li-jun 
Published: March 2018

AbstractGlucose and Glutamine are two essential ingredients for cell growth. However, it remains open for investigation whether there is a general mechanism that coordinates the consumption of glucose and glutamine in cancer cells. Glutamine is mainly metabolized through the glutaminolysis pathway and our previous report indicated that CtBP increases GDH activity and promotes glutaminolysis through repressing the expression of SIRT4, a well-known mitochondrion-located factor that inhibits glutaminolysis pathway. CtBP is known to be a sensor of intracellular metabolic status; we thus hypothesized that a consensus CtBP-SIRT4-GDH axis may mediate the crosstalk between glycolysis and glutaminolysis. Herein, supporting this hypothesis, we observed the coordinated consumption of glucose and glutamine across different cell lines. This coordination was found to be related to CtBP repression activity on SIRT4 expression under high level of glucose but not low glucose level. Low level of glucose supply was found to decrease GDH activity via blocking CtBP dimerization. Mechanically, low glucose also abolished CtBP binding to SIRT4 promoter and the repression of SIRT4 expression. Consistently, the CtBP dimerization inhibitor MTOB mimicked low glucose effects on SIRT4 expression, and GDH activity suggest that CtBP requires high glucose supply to act as a suppressor of SIRT4 gene. In conclusion, we propose that a general molecular pathway composed by CtBP-SIRT4-GDH coordinating the metabolism of glucose and glutamine in cancer cells.
An examination of multiple classes of rare variants in extended families with bipolar disorder
Authors: Toma Claudio , Shaw Alex D. , Allcock Richard J. N. , Heath Anna , Pierce Kerrie D. , Mitchell Philip B. , Schofield Peter R. , Fullerton Janice M. 
Published: March 2018

AbstractBipolar disorder (BD) is a complex psychiatric condition with high heritability, the genetic architecture of which likely comprises both common variants of small effect and rare variants of higher penetrance, the latter of which are largely unknown. Extended families with high density of illness provide an opportunity to map novel risk genes or consolidate evidence for existing candidates, by identifying genes carrying pathogenic rare variants. We performed whole-exome sequencing (WES) in 15 BD families (117 subjects, of whom 72 were affected), augmented with copy number variant (CNV) microarray data, to examine contributions of multiple classes of rare genetic variants within a familial context. Linkage analysis and haplotype reconstruction using WES-derived genotypes enabled exclusion of false-positive single-nucleotide variants (SNVs), CNV inheritance estimation, de novo variant identification and candidate gene prioritization. We found that rare predicted pathogenic variants shared among ≥3 affected relatives were overrepresented in postsynaptic density (PSD) genes (P = 0.002), with no enrichment in unaffected relatives. Genome-wide burden of likely gene-disruptive variants was no different in affected vs. unaffected relatives (P = 0.24), but correlated significantly with age of onset (P = 0.017), suggesting that a high disruptive variant burden may expedite symptom onset. The number of de novo variants was no different in affected vs. unaffected offspring (P = 0.89). We observed heterogeneity within and between families, with the most likely genetic model involving alleles of modest effect and reduced penetrance: a possible exception being a truncating X-linked mutation inIRS4within a family-specific linkage peak. Genetic approaches combining WES, CNV and linkage analyses in extended families are promising strategies for gene discovery.
Scalable total synthesis and comprehensive structure–activity relationship studies of the phytotoxin coronatine
Authors: Littleson Mairi M. , Baker Christopher M. , Dalençon Anne J. , Frye Elizabeth C. , Jamieson Craig , Kennedy Alan R. , Ling Kenneth B. , McLachlan Matthew M. , Montgomery Mark G. , Russell Claire J. , Watson Allan J. B. 
Published: March 2018

AbstractNatural phytotoxins are valuable starting points for agrochemical design. Acting as a jasmonate agonist, coronatine represents an attractive herbicidal lead with novel mode of action, and has been an important synthetic target for agrochemical development. However, both restricted access to quantities of coronatine and a lack of a suitably scalable and flexible synthetic approach to its constituent natural product components, coronafacic and coronamic acids, has frustrated development of this target. Here, we report gram-scale production of coronafacic acid that allows a comprehensive structure–activity relationship study of this target. Biological assessment of a >120 member library combined with computational studies have revealed the key determinants of potency, rationalising hypotheses held for decades, and allowing future rational design of new herbicidal leads based on this template.
Chemical evidence of inter-hemispheric air mass intrusion into the Northern Hemisphere mid-latitudes
Authors: Li S. , Park S. , Lee J.-Y. , Ha K.-J. , Park M.-K. , Jo C. O. , Oh H. , Mühle J. , Kim K.-R. , Montzka S. A. , O’Doherty S. , Krummel P. B. , Atlas E. , Miller B. R. , Moore F. , Weiss R. F. , Wofsy S. C. 
Published: March 2018

AbstractThe East Asian Summer Monsoon driven by temperature and moisture gradients between the Asian continent and the Pacific Ocean, leads to approximately 50% of the annual rainfall in the region across 20–40°N. Due to its increasing scientific and social importance, there have been several previous studies on identification of moisture sources for summer monsoon rainfall over East Asia mainly using Lagrangian or Eulerian atmospheric water vapor models. The major source regions for EASM previously proposed include the North Indian Ocean, South China Sea and North western Pacific. Based on high-precision and high-frequency 6-year measurement records of hydrofluorocarbons (HFCs), here we report a direct evidence of rapid intrusion of warm and moist tropical air mass from the Southern Hemisphere (SH) reaching within a couple of days up to 33°N into East Asia. We further suggest that the combination of direct chemical tracer record and a back-trajectory model with physical meteorological variables helps pave the way to identify moisture sources for monsoon rainfall. A case study for Gosan station (33.25°N, 126.19°E) indicates that the meridional transport of precipitable water from the SH accompanying the southerly/southwesterly flow contributes most significantly to its summer rainfall.
QM/MM study of the reaction mechanism of sulfite oxidase
Authors: Caldararu Octav , Feldt Milica , Cioloboc Daniela , van Severen Marie-Céline , Starke Kerstin , Mata Ricardo A. , Nordlander Ebbe , Ryde Ulf 
Published: March 2018

AbstractSulfite oxidase is a mononuclear molybdenum enzyme that oxidises sulfite to sulfate in many organisms, including man. Three different reaction mechanisms have been suggested, based on experimental and computational studies. Here, we study all three with combined quantum mechanical (QM) and molecular mechanical (QM/MM) methods, including calculations with large basis sets, very large QM regions (803 atoms) and QM/MM free-energy perturbations. Our results show that the enzyme is set up to follow a mechanism in which the sulfur atom of the sulfite substrate reacts directly with the equatorial oxo ligand of the Mo ion, forming a Mo-bound sulfate product, which dissociates in the second step. The first step is rate limiting, with a barrier of 39–49 kJ/mol. The low barrier is obtained by an intricate hydrogen-bond network around the substrate, which is preserved during the reaction. This network favours the deprotonated substrate and disfavours the other two reaction mechanisms. We have studied the reaction with both an oxidised and a reduced form of the molybdopterin ligand and quantum-refinement calculations indicate that it is in the normal reduced tetrahydro form in this protein.
Targeted metabolomic profiling in rat tissues reveals sex differences
Authors: Ruoppolo Margherita , Caterino Marianna , Albano Lucia , Pecce Rita , Di Girolamo Maria Grazia , Crisci Daniela , Costanzo Michele , Milella Luigi , Franconi Flavia , Campesi Ilaria 
Published: March 2018

AbstractSex differences affect several diseases and are organ-and parameter-specific. In humans and animals, sex differences also influence the metabolism and homeostasis of amino acids and fatty acids, which are linked to the onset of diseases. Thus, the use of targeted metabolite profiles in tissues represents a powerful approach to examine the intermediary metabolism and evidence for any sex differences. To clarify the sex-specific activities of liver, heart and kidney tissues, we used targeted metabolomics, linear discriminant analysis (LDA), principal component analysis (PCA), cluster analysis and linear correlation models to evaluate sex and organ-specific differences in amino acids, free carnitine and acylcarnitine levels in male and female Sprague-Dawley rats. Several intra-sex differences affect tissues, indicating that metabolite profiles in rat hearts, livers and kidneys are organ-dependent. Amino acids and carnitine levels in rat hearts, livers and kidneys are affected by sex: male and female hearts show the greatest sexual dimorphism, both qualitatively and quantitatively. Finally, multivariate analysis confirmed the influence of sex on the metabolomics profiling. Our data demonstrate that the metabolomics approach together with a multivariate approach can capture the dynamics of physiological and pathological states, which are essential for explaining the basis of the sex differences observed in physiological and pathological conditions.
Using unique surface patterns of injection moulded plastic components as an image based Physical Unclonable Function for secure component identification
Authors: Wigger Benedikt , Meissner Thomas , Förste Alexander , Jetter Volker , Zimmermann André 
Published: March 2018

AbstractA Physical Unclonable Function uses random and inherent properties of a physical entity and can be used to uniquely identify components e.g., for anti-counterfeiting purposes. In this work we demonstrate that the surface patterns of injection moulded plastic components themselves are inherently unique and hence can be used as a PUF for reliable and secure identification. We further demonstrate that these unique surface patterns are easily accessible since they can be photographed with a simple camera set-up. This is exemplarily demonstrated for two different plastic materials on an overall of 200 injection moulded components. A set of brief experiments further examines the PUF’s robustness towards real life conditions. This approach might be useful for secure identification and authentication of components or a label-free tracking.
Bat-mouse bone marrow chimera: a novel animal model for dissecting the uniqueness of the bat immune system
Authors: Yong Kylie Su Mei , Ng Justin Han Jia , Her Zhisheng , Hey Ying Ying , Tan Sue Yee , Tan Wilson Wei Sheng , Irac Sergio Erdal , Liu Min , Chan Xue Ying , Gunawan Merry , Foo Randy Jee Hiang , Low Dolyce Hong Wen , Mendenhall Ian Hewitt , Chionh Yok Teng , Dutertre Charles-Antoine , Chen Qingfeng , Wang Lin-Fa 
Published: March 2018

AbstractBats are an important animal model with long lifespans, low incidences of tumorigenesis and an ability to asymptomatically harbour pathogens. Currently,in vivostudies of bats are hampered due to their low reproduction rates. To overcome this, we transplanted bat cells from bone marrow (BM) and spleen into an immunodeficient mouse strain NOD-scid IL-2R−/−(NSG), and have successfully established stable, long-term reconstitution of bat immune cells in mice (bat-mice). Immune functionality of our bat-mouse model was demonstrated through generation of antigen-specific antibody response by bat cells following immunization. Post-engraftment of total bat BM cells and splenocytes, bat immune cells survived, expanded and repopulated the mouse without any observable clinical abnormalities. Utilizing bat’s remarkable immunological functions, this novel model has a potential to be transformed into a powerful platform for basic and translational research.
Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131
Authors: Mora Azucena , García-Peña Francisco Javier , Alonso María Pilar , Pedraza-Diaz Susana , Ortega-Mora Luis Miguel , Garcia-Parraga Daniel , López Cecilia , Viso Susana , Dahbi Ghizlane , Marzoa Juan , Sergeant Martin J. , García Vanesa , Blanco Jorge 
Published: March 2018

AbstractThere is growing concern about the spreading of human microorganisms in relatively untouched ecosystems such as the Antarctic region. For this reason, three pinniped species (Leptonychotes weddellii, Mirounga leonina and Arctocephalus gazella) from the west coast of the Antartic Peninsula were analysed for the presence of Escherichia spp. with the recovery of 158 E. coli and three E. albertii isolates. From those, 23 harboured different eae variants (α1, β1, β2, ε1, θ1, κ, ο), including a bfpA-positive isolate (O49:H10-A-ST206, eae-k) classified as typical enteropathogenic E. coli. Noteworthy, 62 of the 158 E. coli isolates (39.2%) exhibited the ExPEC status and 27 (17.1%) belonged to sequence types (ST) frequently occurring among urinary/bacteremia ExPEC clones: ST12, ST73, ST95, ST131 and ST141. We found similarities >85% within the PFGE-macrorrestriction profiles of pinniped and human clinic O2:H6-B2-ST141 and O16:H5/O25b:H4-B2-ST131 isolates. The in silico analysis of ST131 Cplx genomes from the three pinnipeds (five O25:H4-ST131/PST43-fimH22-virotype D; one O16:H5-ST131/PST506-fimH41; one O25:H4-ST6252/PST9-fimH22-virotype D1) identified IncF and IncI1 plasmids and revealed high core-genome similarities between pinniped and human isolates (H22 and H41 subclones). This is the first study to demonstrate the worrisome presence of human-associated E. coli clonal groups, including ST131, in Antarctic pinnipeds.
Induced clustering of Escherichia coli by acoustic fields
Authors: Gutiérrez-Ramos Salomé , Hoyos Mauricio , Ruiz-Suárez J. C. 
Published: March 2018

AbstractBrownian or self-propelled particles in aqueous suspensions can be trapped by acoustic fields generated by piezoelectric transducers usually at frequencies in the megahertz. The obtained confinement allows the study of rich collective behaviours like clustering or spreading dynamics in microgravity-like conditions. The acoustic field induces the levitation of self-propelled particles and provides secondary lateral forces to capture them at nodal planes. Here, we give a step forward in the field of confined active matter, reporting levitation experiments of bacterial suspensions of Escherichia coli. Clustering of living bacteria is monitored as a function of time, where different behaviours are clearly distinguished. Upon the removal of the acoustic signal, bacteria rapidly spread, impelled by their own swimming. Nevertheless, long periods of confinement result in irreversible bacteria entanglements that could act as seeds for levitating bacterial aggregates.
The epicardium obscures interpretations on endothelial-to-mesenchymal transition in the mouse atrioventricular canal explant assay
Authors: Criem Nathan , Zwijsen An 
Published: March 2018

AbstractAtrioventricular septal defects often result from impaired endocardial cushion development. Endothelial-to-mesenchymal transition (EndoMT) is a critical event in endocardial cushion development that initiates in the atrioventricular canal (AVC). In ex vivo EndoMT studies, mouse AVCs are flat-mounted on a collagen gel. In the explant outgrowths, the ratio of elongated spindle-like mesenchymal cells over cobblestone-shaped cells, generally considered as endothelial cells, reflects EndoMT. Using this method, several key signalling pathways have been attributed important functions during EndoMT. Using genetic lineage tracing and cell-type-specific markers, we show that monolayers of cobblestone-shaped cells are predominantly of epicardial rather than endothelial origin. Furthermore, this epicardium is competent to undergo mesenchymal transition. Contamination by epicardium is common and inherent as this tissue progressively attaches to AVC myocardium. Inhibition of TGFβ signalling, previously shown to blunt EndoMT, caused an enrichment in epicardial monolayers. The presence of epicardium thus confounds interpretations of EndoMT signalling pathways in this assay. We advocate to systematically use lineage tracers and cell-type-specific markers on stage-matched AVC explants. Furthermore, a careful reconsideration of earlier studies on EndoMT using this explant assay may identify unanticipated epicardial effects and/or the presence of epicardial-to-mesenchymal transition (EpiMT), which would alter the interpretation of results on endothelial-to-mesenchymal transition.
Corneal nerve fiber size adds utility to the diagnosis and assessment of therapeutic response in patients with small fiber neuropathy
Authors: Brines Michael , Culver Daniel A. , Ferdousi Maryam , Tannemaat Martijn R. , van Velzen Monique , Dahan Albert , Malik Rayaz A. 
Published: March 2018

AbstractSmall fiber neuropathy (SFN) is a common feature of many inflammatory diseases, often presenting with pain and disability. SFN is diagnosed using symptoms, thermal threshold testing, and intra-epidermal nerve fiber quantification. Corneal confocal microscopy (CCM) is an ophthalmic imaging technique which non-invasively quantifies corneal nerve fiber (CNF) density, branch density and length, and has comparable diagnostic and superior ability to identify nerve regeneration compared to skin biopsy. CNF size (width and area) depends upon the number of fibers within each nerve, as well as pathology (e.g., swelling), and may provide additional sensitivity to diagnose SFN and identify nerve repair. We have compared the utility of the standard CCM variables employed to CNF size in patients with diabetic sensorimotor polyneuropathy or sarcoidosis-associated SFN, and in patients with SFN following cibinetide administration, an agent which promotes nerve repair. The results show that: 1) CNF width distribution and area depend upon neuropathy severity; 2) CNF area, density, branch density and length possess comparable discriminatory power for diagnosing neuropathy; 3) CNF area is related to length by a quadratic function which is predictive for both healthy subjects and those with SFN; 4) CNF area is a useful variable for quantifying change in CNF morphology.
A novel synthetic peptide microarray assay detects Chlamydia species-specific antibodies in animal and human sera
Authors: Sachse Konrad , Rahman Kh. Shamsur , Schnee Christiane , Müller Elke , Peisker Madlen , Schumacher Thomas , Schubert Evelyn , Ruettger Anke , Kaltenboeck Bernhard , Ehricht Ralf 
Published: March 2018

AbstractSerological analysis ofChlamydia (C.)spp. infections is still mainly based on micro-immunofluorescence and ELISA. To overcome the limitations of conventional serology, we have designed a novel microarray carrying 52 synthetic peptides representing B-cell epitopes from immunodominant proteins of all 11 chlamydial species. The new assay has been validated using monospecific mouse hyperimmune sera. Subsequently, serum samples from cattle, sheep and humans with a known history of chlamydial infection were examined. For instance, the specific humoral response of sheep to treatment with aC. abortusvaccine has been visualized against a background ofC. pecorumcarriership. In samples from humans, dual infection withC. trachomatisandC. pneumoniaecould be demonstrated. The experiments revealed that the peptide microarray assay was capable of simultaneously identifying specific antibodies to eachChlamydiaspp. The actual assay represents an open platform test that can be complemented through future advances inChlamydiaproteome research. The concept of the highly parallel multi-antigen microarray proven in this study has the potential to enhance our understanding of antibody responses by defining not only a single quantitative response, but also the pattern of this response. The added value of using peptide antigens will consist in unprecedented serodiagnostic specificity.
Non-invasive coronary physiology based on computational analysis of intracoronary transluminal attenuation gradient
Authors: Bae Yong Gyun , Hwang Seung Tae , Han Huan , Kim Sung Mok , Kim Hyung-Yoon , Park Il , Lee Joo Myung , Moon Young-June , Choi Jin-Ho 
Published: March 2018

AbstractInvasive procedure is a prerequisite for studying coronary physiology. We established the measurement of non-invasive physiological parameters including coronary blood flow (CBF), flow velocity, and microvascular resistance using coronary computed tomography angiography (CCTA). Vessel-specific CBF was derived from transluminal attenuation flow encoding (TAFE) and then tested using three separate datasets consisted of computational simulation, human perfusion CT, and human CCTA. TAFE-derived CBF correlated well with measured vessel-specific myocardial blood flow and CBF. TAFE-derived CBF per myocardial mass consistently decreased with the progressive severity of stenosis, and it was found to better to detect significant stenosis than transluminal attenuation gradient (TAG). With the addition of vessel anatomy, TAFE-derived CBF could calculate flow velocity and microvascular resistance. The results of non-invasively acquired parameters according to the severity of stenosis were similar to those obtained through invasive physiology studies. Our study demonstrated that non-invasive comprehensive coronary physiology parameters can be derived from CCTA without any pre-specified condition or performing complex heavy computational processes. Our findings are expected to expand the clinical coverage of CCTA and coronary physiology.