bluuebunny/crossref_metadata_2025_split huggingface dataset:
Key comparison BIPM.RI(I)-K2 of the air-kerma standards of the NPL, UK, and the BIPM in low-energy x-rays
Authors: Burns D T , Kessler C , Kelly M , Maughan D J , Sander T 
Published: April 2019

A key comparison has been made between the air-kerma standards of the NPL, UK and the BIPM in the low-energy x-ray range. The results indicate a progressive drift of the NPL standard, now lower than the BIPM standard by around 1 part in 102. The expanded uncertainty (k = 2) of the comparison is 4.2 parts in 103. The results are analysed and presented in terms of degrees of equivalence, suitable for entry in the BIPM key comparison database.

Main text

To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.
The final report has been peer-reviewed and approved for publication by the CCRI, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).
The message of Methodism
Authors: Abraham William J. 
Published: April 2019

Abstract
‘The message of Methodism’ refers to the complex network of doctrines that became essential to the teachings and identity of Methodism and considers its canonical sermons and crucial elements. Methodism is not a cult of John Wesley; it is a whole new vision of Christianity. While it clearly bears the imprint of John Wesley and the early leaders, it had to develop the doctrinal and intellectual resources to carry it across space and time. Its initial message was certainly furnished by Wesley, but it was not limited to Wesley. Moreover, given its mission to spread Christianity near and far, it necessarily made adjustments as best it could as it spread across the world.
The search for credible alternatives
Authors: Abraham William J. 
Published: April 2019

Abstract
The search for a viable alternative to classical Methodist teaching was taken up in earnest in the first half of the 20th century. If there was a failure of nerve in the effort to sustain the riches of the tradition, there was no lack of confidence in pursuing the quest for a better future intellectually. The basic strategy was to reach outside the boundaries of Methodism and find fitting materials that would replace the earlier orthodoxy. ‘The search for credible alternatives’ describes the work and impact of Albert Outler in the 1960s, new developments over the last fifty years, and present-day disputes and problems within Methodism.
Use of Antihypertensive Medications and Survival Rates for Breast, Colorectal, Lung, or Stomach Cancer
Authors: Cui Yong , Wen Wanqing , Zheng Tao , Li Honglan , Gao Yu-Tang , Cai Hui , You Mingrong , Gao Jing , Yang Gong , Zheng Wei , Xiang Yong-Bing , Shu Xiao-Ou 
Published: April 2019

AbstractUsing time-dependent Cox regression models, we examined associations of common antihypertensive medications with overall cancer survival (OS) and disease-specific survival (DSS), with comprehensive adjustment for potential confounding factors. Participants were from the Shanghai Women’s Health Study (1996–2000) and Shanghai Men’s Health Study (2002–2006) in Shanghai, China. Included were 2,891 incident breast, colorectal, lung, and stomach cancer cases. Medication use was extracted from electronic medical records. With a median 3.4-year follow-up after diagnosis (interquartile range, 1.0–6.3), we found better outcomes among users of angiotensin II receptor blockers with colorectal cancer (OS: adjusted hazard ratio (HR) = 0.62, 95% confidence interval (CI): 0.44, 0.86; DSS: adjusted HR = 0.61, 95% CI: 0.43, 0.87) and stomach cancer (OS: adjusted HR = 0.62, 95% CI: 0.41, 0.94; DSS: adjusted HR = 0.63, 95% CI: 0.41, 0.98) and among users of β-adrenergic receptor blockers with colorectal cancer (OS: adjusted HR = 0.50, 95% CI: 0.35, 0.72; DSS: adjusted HR = 0.50, 95% CI: 0.34, 0.73). Better survival was also found for calcium channel blockers (DSS: adjusted HR = 0.67, 95% CI: 0.47, 0.97) and diuretics (OS: adjusted HR = 0.66, 95% CI: 0.45, 0.96; DSS: adjusted HR = 0.57, 95% CI: 0.38, 0.85) with stomach cancer. Our findings suggest angiotensin II receptor blockers, β-adrenergic receptor blockers, and calcium channel blockers might be associated with improved survival outcomes of gastrointestinal cancers.
Functional and chemical characterization of XAF: a heat-stable plant polymer that activates xyloglucan endotransglucosylase/hydrolase (XTH)
Authors: Nguyen-Phan Tu C , Fry Stephen C 
Published: April 2019

AbstractBackground and AimsXyloglucan endotransglucosylase/hydrolase (XTH) proteins that possess xyloglucan endotransglucosylase (XET) activity contribute to cell-wall assembly and remodelling, orchestrating plant growth and development. Little is known about in-vivo XET regulation, other than at the XTH transcriptional level. Plants contain ‘cold-water-extractable, heat-stable polymers’ (CHPs) which are XTH-activating factors (XAFs) that desorb and thereby activate wall-bound XTHs. Because XAFs may control cell-wall modification in vivo, we have further explored their nature.MethodsMaterial was cold-water-extracted from 25 plant species; proteins were precipitated by heat-denaturation, then CHP was ethanol-precipitated. For XAF assays, CHP (or sub-fractions thereof) was applied to washed Arabidopsis thaliana cell walls, and the enzymes thus solubilized were assayed radiochemically for XET activity. In some experiments, the CHP was pre-treated with trifluoroacetic acid (TFA), alkali (NaOH) or glycanases.Key ResultsCHP specifically desorbed wall-bound XTHs, but not β-glucosidases, phosphatases or peroxidases. CHP preparations from 25 angiosperms all possessed XAF activity but had no consistent monosaccharide composition. Of 11 individual plant polymers tested, only gum arabic and tamarind xyloglucan were XAF-active, albeit less so than CHP. On gel-permeation chromatography, XAF-active cauliflower CHP eluted with a molecular weight of ~7000–140 000, although no specific sugar residue(s) co-eluted exactly with XAF activity. Cauliflower XAF activity survived cold alkali and warm dilute TFA (which break ester and glycofuranosyl linkages, respectively), but was inactivated by hot 2 m TFA (which breaks glycopyranosyl linkages). Cauliflower XAF activity was remarkably stable to diverse glycanases and glycosidases.ConclusionsXAFs are naturally occurring heat-stable polymers that specifically desorb (thereby activating) wall-bound XTHs. Their XAF activity considerably exceeds that of gum arabic and tamarind xyloglucan, and they were not identifiable as any major plant polysaccharide. We propose that XAF is a specific, minor, plant polymer that regulates xyloglucan transglycosylation in vivo, and thus wall assembly and restructuring.
The infusion of menthol into the esophagus evokes cold sensations in healthy subjects but induces heartburn in patients with gastroesophageal reflux disease (GERD)
Authors: Banovcin Peter , Duricek Martin , Zatko Tomas , Liptak Peter , Hyrdel Rudolf , Kollarik Marian 
Published: April 2019

SUMMARY
Recent studies in animal models have reported that some afferent fibers innervating the esophagus express the cold receptor TRPM8. In the somatosensory system the stimulation of TRPM8 leads to cold sensations and in certain circumstances alleviates pain. It is therefore hypothesized in this paper that the esophageal infusion of the TRPM8 activator menthol evokes cold sensations from the esophagus and alleviates heartburn in humans. The esophageal infusion of menthol (3 mM, 20 min) evoked cold sensations in 11 of 12 healthy subjects. In striking contrast, the esophageal infusion of menthol evoked heartburn in 10 of 10 patients with gastroesophageal reflux disease (GERD). In healthy subjects the cold sensation evoked by menthol was perceived only as a minor discomfort as evaluated by the visual analog scale (VAS score 1.9 ± 0.3 on the scale 1–10). However, in patients with GERD the menthol-induced heartburn was perceived as painful (VAS score 5.6 ± 0.6, P &lt; 0.01 compared to healthy subjects). It is concluded that the sensations evoked by esophageal infusion of menthol change from relatively nonpainful cold sensations in healthy subjects to painful heartburn sensations in patients with GERD. These qualitative and quantitative changes indicate substantial alterations in afferent signaling mediating sensations from the esophagus in patients with GERD.
It’s not ageing, stupid: why population ageing won’t bankrupt health systems
Authors: Jayawardana Sahan , Cylus Jonathan , Mossialos Elias 
Published: April 2019

Abstract
An ageing population is often viewed as a factor that will increase healthcare expenditures (HCE) to unsustainable levels. With nearly half the disease burden in high-income countries arising in older people, there are concerns that caring for a burgeoning older population that is sick and dependent on support will not be possible. The aim of this narrative review is to examine how population ageing is likely to affect future HCE growth. We find that despite the increasing share of older people over the next few decades, the changing age-mix will not be a significant driver of HCE growth. While older people, on average, incur higher per person healthcare spending than younger people, the proportions of the population at the oldest and most costly age groups increase very slowly over time. In the European Union, we estimate the changing age-mix to result in the increase of the average annual growth in per person health spending by no more than 0.6 additional percentage points per year between 2015 and 2050. Therefore, price growth and technological advancements, independent of population ageing, will be the main contributors to future HCE growth.
Strategy instruction for successful language tandems
Authors: Webster Simon 
Published: April 2019

AbstractEducational institutions are placing increased value on language tandems owing to the language development opportunities they offer for learners of modern languages. Where these learners lack autonomous language learning strategies, however, the results can be disappointing, with limited linguistic development taking place. This paper reports on recent research into the language tandem experiences of international study abroad students during their period of study at a British university. When it became apparent that the students lacked skills in managing their language tandems to meet their learning needs, strategy instruction was introduced to facilitate more effective language learning strategy use. The findings suggest that such an intervention can significantly increase learners’ ability to create a language tandem which meets their learning needs.
Women have lower chances than men to be resuscitated and survive out-of-hospital cardiac arrest
Authors: Blom Marieke T , Oving Iris , Berdowski Jocelyn , van Valkengoed Irene G M , Bardai Abdenasser , Tan Hanno L 
Published: April 2019

Abstract

Aims
Previous studies on sex differences in out-of-hospital cardiac arrest (OHCA) had limited scope and yielded conflicting results. We aimed to provide a comprehensive overall view on sex differences in care utilization, and outcome of OHCA.

Methods and results
We performed a population-based cohort-study, analysing all emergency medical service (EMS) treated resuscitation attempts in one province of the Netherlands (2006–2012). We calculated odds ratios (ORs) for the association of sex and chance of a resuscitation attempt by EMS, shockable initial rhythm (SIR), and in-hospital treatment using logistic regression analysis. Additionally, we provided an overview of sex differences in overall survival and survival at successive stages of care, in the entire study population and in patients with SIR. We identified 5717 EMS-treated OHCAs (28.0% female). Women with OHCA were less likely than men to receive a resuscitation attempt by a bystander (67.9% vs. 72.7%; P &lt; 0.001), even when OHCA was witnessed (69.2% vs. 73.9%; P &lt; 0.001). Women who were resuscitated had lower odds than men for overall survival to hospital discharge [OR 0.57; 95% confidence interval (CI) 0.48–0.67; 12.5% vs. 20.1%; P &lt; 0.001], survival from OHCA to hospital admission (OR 0.88; 95% CI 0.78–0.99; 33.6% vs. 36.6%; P = 0.033), and survival from hospital admission to discharge (OR 0.49, 95% CI 0.40–0.60; 33.1% vs. 51.7%). This was explained by a lower rate of SIR in women (33.7% vs. 52.7%; P &lt; 0.001). After adjustment for resuscitation parameters, female sex remained independently associated with lower SIR rate.

Conclusion
In case of OHCA, women are less often resuscitated by bystanders than men. When resuscitation is attempted, women have lower survival rates at each successive stage of care. These sex gaps are likely explained by lower rate of SIR in women, which can only partly be explained by resuscitation characteristics.
Less dementia and stroke in low-risk patients with atrial fibrillation taking oral anticoagulation
Authors: Friberg Leif , Andersson Tommy , Rosenqvist Mårten 
Published: April 2019

Abstract

Aims
To investigate if patients with atrial fibrillation (AF) without clear indication for oral anticoagulant (OAC) due to perceived low stroke risk may benefit from OAC treatment when also dementia and intracerebral bleeding risks are considered.

Methods and results
Retrospective study of cross-matched national registries of all individuals in Sweden with a hospital diagnosis of AF between 2006 and 2014 (n = 456 960). Exclusion was made of patients with a baseline CHA2DS2-VASc score &gt;1, not counting female sex, and of patients with previous diagnosis of dementia or intracranial bleeding. After exclusions, 91 254 patients remained in the study of whom 43% used OAC at baseline. Propensity score matching and falsification endpoints were used. Treatment with OAC was associated with lower risk of dementia after adjustment for death as a competing risk [subhazard ratio (sHR) 0.62 with 95% confidence interval (CI) 0.48–0.81]. Regarding the composite brain protection endpoint, OAC treatment was associated with an overall 12% lower risk (sHR 0.88, CI 0.72–1.00). This apparent benefit was restricted to patients aged &gt;65 years, whereas OAC treatment of patients &lt;60 years of age without risk factors appeared to do more harm than good.

Conclusion
Low-risk AF patients who take OAC have lower risk of dementia than those who do not use OAC. Patients age &gt;65 years appear to benefit from OAC treatment irrespective of stroke risk score.
Single-cell transcriptome analyses reveal novel targets modulating cardiac neovascularization by resident endothelial cells following myocardial infarction
Authors: Li Ziwen , Solomonidis Emmanouil G , Meloni Marco , Taylor Richard S , Duffin Rodger , Dobie Ross , Magalhaes Marlene S , Henderson Beth E P , Louwe Pieter A , D’Amico Gabriela , Hodivala-Dilke Kairbaan M , Shah Ajay M , Mills Nicholas L , Simons Benjamin D , Gray Gillian A , Henderson Neil C , Baker Andrew H , Brittan Mairi 
Published: April 2019

Abstract

Aims
A better understanding of the pathways that regulate regeneration of the coronary vasculature is of fundamental importance for the advancement of strategies to treat patients with heart disease. Here, we aimed to investigate the origin and clonal dynamics of endothelial cells (ECs) associated with neovascularization in the adult mouse heart following myocardial infarction (MI). Furthermore, we sought to define murine cardiac endothelial heterogeneity and to characterize the transcriptional profiles of pro-angiogenic resident ECs in the adult mouse heart, at single-cell resolution.

Methods and results
An EC-specific multispectral lineage-tracing mouse (Pdgfb-iCreERT2-R26R-Brainbow2.1) was used to demonstrate that structural integrity of adult cardiac endothelium following MI was maintained through clonal proliferation by resident ECs in the infarct border region, without significant contributions from bone marrow cells or endothelial-to-mesenchymal transition. Ten transcriptionally discrete heterogeneous EC states, as well as the pathways through which each endothelial state is likely to enhance neovasculogenesis and tissue regeneration following ischaemic injury were defined. Plasmalemma vesicle-associated protein (Plvap) was selected for further study, which showed an endothelial-specific and increased expression in both the ischaemic mouse and human heart, and played a direct role in regulating human endothelial proliferation in vitro.

Conclusion
We present a single-cell gene expression atlas of cardiac specific resident ECs, and the transcriptional hierarchy underpinning endogenous vascular repair following MI. These data provide a rich resource that could assist in the development of new therapeutic interventions to augment endogenous myocardial perfusion and enhance regeneration in the injured heart.
Time-to-isolation-guided cryoballoon ablation reduces oesophageal and mediastinal alterations detected by endoscopic ultrasound: results of the MADE-PVI trial
Authors: Cordes Friederike , Ellermann Christian , Dechering Dirk G , Frommeyer Gerrit , Kochhäuser Simon , Lange Philipp S , Pott Christian , Lenze Frank , Schmidt Hartmut , Ullerich Hansjoerg , Eckardt Lars 
Published: April 2019

Abstract

Aims
Cryoballoon ablation is safe and efficient for achieving pulmonary vein isolation (PVI) in atrial fibrillation. Structural oesophago-mediastinal lesions, which seem to be associated with an increased risk of the lethal complication of an atrio-oesophageal fistula, have been described. MADE-PVI (Mediastino-oesophageal Alterations Detected by Endosonography after PVI) aimed at evaluating safety of cryoballoon PVI in relation to two different freeze protocols. As time-to-isolation-(TTI)-guided protocol has been reported to be as effective as conventional ‘two freeze protocol’, we hypothesized a TTI-guided protocol causes less oesophago-mediastinal lesions.

Methods and results
Seventy consecutive patients were scheduled for cryoballoon (2nd generation) PVI employing either a conventional protocol (n = 35: 2 × 180 s per vein) or a TTI-guided approach (n = 35: TTI + 120 s per vein or 1 × 180 s in case TTI could not be measured). Oesophagogastroduodenoscopy and endoscopic ultrasound, assessing oesophago-mediastinal alterations (e.g. ulceration, oedema) were performed blinded prior and post-ablation. Post-interventional mediastinal oedematous alterations were detected in 70% with a mean diameter of 14 mm (±0.9 mm), while only 15% revealed large mediastinal oedema >20 mm. Oesophageal lesions due to PVI occurred in 5%. Freeze protocols had a distinct impact on oesophago-mediastinal alterations as mean diameter and frequency of large oedema were significantly increased in patients after conventional protocol PVI (17 mm vs. 11 mm; 26% vs. 6%). Furthermore, every oesophageal lesion was detected in patients with conventional protocol (9%). No major complication occurred in either group.

Conclusion
The present prospective study demonstrates a significant impact of freeze protocol on oesophago-mediastinal alterations. A TTI-guided protocol reduces mediastino-oesophageal lesions and may reduce short- and long-term complications of cryoballoon PVI.
Nitrogen dynamics of decomposing Scots pine needle litter depends on colonizing fungal species
Authors: Baskaran Preetisri , Ekblad Alf , Soucémarianadin Laure N , Hyvönen Riitta , Schleucher Jürgen , Lindahl Björn D 
Published: April 2019

ABSTRACT
In boreal ecosystems plant production is often limited by low availability of nitrogen. Nitrogen retention in below-ground organic pools plays an important role in restricting recirculation to plants and thereby hampers forest production. Saprotrophic fungi are commonly assigned to different decomposer strategies, but how these relate to nitrogen cycling remains to be understood. Decomposition of Scots pine needle litter was studied in axenic microcosms with the ligninolytic litter decomposing basidiomycete Gymnopus androsaceus or the stress tolerant ascomycete Chalara longipes. Changes in chemical composition were followed by 13C CP/MAS NMR spectroscopy and nitrogen dynamics was assessed by the addition of a 15N tracer. Decomposition by C. longipes resulted in nitrogen retention in non-hydrolysable organic matter, enriched in aromatic and alkylic compounds, whereas the ligninolytic G. androsaceus was able to access this pool, counteracting nitrogen retention. Our observations suggest that differences in decomposing strategies between fungal species play an important role in regulating nitrogen retention and release during litter decomposition, implying that fungal community composition may impact nitrogen cycling at the ecosystem level.
Unexpected connections between type VI-B CRISPR-Cas systems, bacterial natural competence, ubiquitin signaling network and DNA modification through a distinct family of membrane proteins
Authors: Makarova Kira S , Gao Linyi , Zhang Feng , Koonin Eugene V 
Published: April 2019

ABSTRACT
In addition to core Cas proteins, CRISPR-Cas loci often encode ancillary proteins that modulate the activity of the respective effectors in interference. Subtype VI-B1 CRISPR-Cas systems encode the Csx27 protein that down-regulates the activity of Cas13b when the type VI-B locus is expressed in Escherichia coli. We show that Csx27 belongs to an expansive family of proteins that contain four predicted transmembrane helices and are typically encoded in predicted operons with components of the bacterial natural transformation machinery, multidomain proteins that consist of components of the ubiquitin signaling system and proteins containing the ligand-binding WYL domain and a helix-turn-helix domain. The Csx27 family proteins are predicted to form membrane channels for ssDNA that might comprise the core of a putative novel, Ub-regulated system for DNA uptake and, possibly, degradation. In addition to these associations, a distinct subfamily of the Csx27 family appears to be a part of a novel, membrane-associated system for DNA modification. In Bacteroidetes, subtype VI-B1 systems might degrade nascent transcripts of foreign DNA in conjunction with its uptake by the bacterial cell. These predictions suggest several experimental directions for the study of type VI CRISPR-Cas systems and distinct mechanisms of foreign DNA uptake and degradation in bacteria.
Bias in common catch-curve methods applied to age frequency data from fish surveys
Authors: Nelson Gary A 
Published: April 2019

Abstract
Catch curve analysis is often used in data-limited fisheries stock assessments to estimate total instantaneous mortality (Z). There are now six catch-curve methods available in the literature: the Chapman–Robson, linear regression, weighted linear regression, Heincke, generalized Poisson linear, and random-intercept Poisson linear mixed model. An assumption shared among the underyling probability models of these estimators is that fish collected for ageing are sampled from the population by simple random sampling. This type of sampling is nearly impossible in fisheries research because populations are sampled in surveys that use gears that capture individuals in clusters and often fish for ageing are selected from multi-stage sampling. In this study, I explored the effects of multi-stage cluster sampling on the bias of the estimates of Z and their associated standard errors. I found that the generalized Poisson linear model and the Chapman–Robson estimators were the least biased, whereas the random-intercept Poisson linear mixed model was the most biased under a wide range of simulation scenarios that included different levels of recruitment variation, intra-cluster correlation, sample sizes, and methods used to generate age frequencies. Standard errors of all estimators were under-estimated in almost all cases and should not be used in statistical comparisons.