bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioinspired polydopamine coating‐assisted electrospun polyurethane‐graphene oxide nanofibers for bone tissue engineering application
Authors: Ghorbani Farnaz , Zamanian Ali , Aidun Amir 
Published: February 2019

ABSTRACTIt is inevitable to replace the tissues and organs that have been malfunctioning due to trauma or difference diseases, so tissue engineering can be a promising approach in case of regeneration. In this investigation, electrospun polyurethane‐graphene oxide scaffold was coated with polydopamine (PDA) by immersing the constructs in dopamine hydrochloride solution under the alkaline condition for 24 h. The present study aimed to investigate the synergic effects of graphene oxide (GO) and PDA on the osteogenic expression. Field emission scanning electron microscopy, Fourier transform infrared spectrum, and X‐ray diffraction (XRD) proved the synthesis of GO. Water drop contact angle and swelling absorption test indicated significantly enhancement in hydrophilicity after coating the scaffolds with PDA. Besides, PDA induced more ability of mineralization bone‐like component. According to the results, cell attachment and proliferation were greatly increased in coated constructs. Alkaline phosphatase expression was also increased on the PDA‐deposited polymeric scaffolds. In general, these characteristics suggest that the PDA‐coated polyurethane‐GO scaffolds are an appropriate substrate for in vivo studies and the bone regeneration. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47656.
Efficient Enhancement of Electron Transport and Collection Capability in PTB7:PC71BM‐based Solar Cells Enabled by Sulforhodamine Cathode Interlayers
Authors: Song Wei , Li Wang , Peng Ruixiang , Fanady Billy , Huang Jiaming , Zhu Wenqing , Xie Lingchao , Lei Tao , Ge Ziyi 
Published: February 2019

AbstractDevelopment of low‐cost water‐/alcohol‐soluble interfacial materials is a crucial issue to promote the commercialization of polymer solar cells (PSCs). Herein, two derivatives of low‐cost rhodamine, called sulforhodamine 101 (SR101) and sulforhodamine B (SRB), are applied as cathode interfacial layers (CILs) to effectively improve the charge‐carrier transportation and collection, reduce the work function (WF) of Al counter electrode, and decrease the series resistance and charge recombination in the PSCs. As a result, SR101‐based devices show excellent performance with the highest power conversion efficiency (PCE) of 9.10 %, superior to that of both the control devices with MeOH/Al and Ca/Al. Notably, sulforhodamine is commercially available with low cost and great solution‐processability. This work demonstrates that sulforhodamine has a great potential as a CIL material,which is suitable for the large‐area fabrication process and commercialization of highly efficient PSCs.
Influence of Backbone Chlorination on the Electronic Properties of Diketopyrrolopyrrole (DPP)‐Based Dimers
Authors: Xue Ning , Wei Yuan , Zhang Guowei , Liu Lele , Zhang Lei 
Published: February 2019

AbstractChlorination of π‐conjugated backbones is garnering great interest because of fine‐tuning electronic properties of conjugated materials for organic devices. Herein we report a synthesis of thiophene‐based diketopyrrolopyrrole (DPP) dimers and their chlorinated counterparts by introducing a chlorine atom in the outer thiophene ring to investigate the influence of the chlorination on charge transport. The backbone chlorination lowers both the HOMO and the LUMO of the dimers and leads to a blue‐shift of maximum absorption in compared to unsubstituted counterparts. X‐ray analysis reveals that the chlorine atom prompts the outer thiophene ring out of the planarity of the backbone with a relatively large torsional angle. The chlorinated dimers exhibit slipped one‐dimensional packing decorated with multiple intermolecular interactions, because of a combination of a negative inductive effect and a positive mesomeric effect of the halogen atom, which might facilitate charge transport within the oligomeric backbones. The mobility in the single‐crystal OFET devices of the chlorinated dimers is up to 1.5 cm2 V−1 s−1, which is two times higher than that of the non‐chlorinated DPP dimers. Our results indicate that the chlorine atom plays a key role in directing non‐covalent interactions to lock the slipped stacks, enabling electronic coupling between adjacent molecules for efficient charge transport. In addition, our results also demonstrate that these DPP dimers with straight n‐octyl chains exhibit higher mobilities than the dimers with branched 2‐ethylhexyl chains.
Autism prevalence and outcomes in older adults
Authors: Robison John Elder 
Published: February 2019

Recent studies of mortality, illness, and suicide among autistic adults paint an alarming picture. Autistic people appear to die much earlier than the general population, and they seem to be far more vulnerable to a surprising range of medical problems. Suicide and depression seem far more common than in the general population. If correct, that suggests an older autistic population in silent crisis, with few if any supports. If so, older autistic people should be a focus for public health and human service agencies. But is the picture complete? Autism researchers ask for answers, identifying problems and their scope. This article discusses the limitations of our adult autism knowledge, and the challenges we will face studying adults. Researching and ultimately serving older autistic adults presents a unique set of problems that have not yet been addressed by scientists or clinicians. Autism Res 2019, 12: 370–374 © 2019 International Society for Autism Research, Wiley Periodicals, Inc.Lay SummaryPublic policy toward autistic people is driven by data. Most autism data to date have been derived from and about children, because autism tends to be identified and supported in the public school system. This has created a public perception of autism as a childhood problem. In fact, autism is a lifelong difference or disability, and recent studies suggest serious overlooked concerns for autistic adults. This commentary discusses how we have evaluated adult autism so far, limitations of our knowledge, and how we might evaluate adult needs going forward. The commentary makes a case for specific new adult prevalence and outcome studies to inform public policy.
Thiourea Bismuth Iodide: Crystal Structure, Characterization and High Performance as an Electrode Material for Supercapacitors
Authors: Li Tianyue , Mallows John , Adams Keir , Nichol Gary S. , Thijssen Job H. J. , Robertson Neil 
Published: February 2019

AbstractThis paper reports on the synthesis, crystal structure and application of a novel hybrid bismuth‐halide complex: (CN2SH5)3BiI6 (TBI) for supercapacitor applications, featuring merits including high areal capacitance, low cost, solution‐processability and non‐toxicity. Single crystal X‐ray diffraction reveals that TBI crystallizes in the monoclinic system, with discrete [BiI6]3− octahedra as the inorganic motif. Utilizing TBI as the active supercapacitor electrode material with carbon cloth current collector and aqueous NaClO4 electrolyte, an electrode areal capacitance of 3.32 F/cm2 and a systemic specific capacitance of 1030 F/g was achieved when the device operates as an electric double‐layer capacitor (EDLC). The supercapacitor device shows excellent capacitance retention even after 5,000 charge‐discharge cycles. The powder XRD patterns, Raman spectroscopy and SEM images of TBI electrodes were compared before and after the cycling test to demonstrate the material stability and investigate the film morphology.
ROR1 associates unfavorable prognosis and promotes lymphoma growth in DLBCL by affecting PI3K/Akt/mTOR signaling pathway
Authors: Mao Yuan , Xu Li , Wang Jun , Zhang Louqian , Hou Nan , Xu Juqing , Wang Lin , Yang Shu , Chen Yan , Xiong Lin , Zhu Jin , Fan Weifei , Xu Jiaren 
Published: February 2019

AbstractThe receptor‐tyrosine‐kinase (RTK)‐like orphan receptor 1 (ROR1) is a transmembrane glycoprotein regarded as a tumor‐associated antigen. ROR1 plays an important role in cancer development, but the detailed function of ROR1 in diffuse large B‐cell lymphoma (DLBCL) remains unclear. In this study, we first detected ROR1 expression and evaluated the relationship between ROR1 expression and the clinicopathological characteristics of DLBCL patients. Next we employed shRNA‐mediated knockdown of ROR1 in DLBCL cell line to explore the characteristics of ROR1 in DLBCL development both in vitro and in vivo. The results showed a significantly higher level of ROR1 in DLBCL tissues than in lymphatic hyperplasia tissues. High ROR1 expression was correlated with unfavorable prognosis in DLBCL patients. Furthermore, ROR1 knockdown inhibited the growth and induced the apoptosis in DLBCL cells and xenografts. In addition, shROR1 inhibited activation of the PI3K/Akt/mTOR signaling pathway, both in vitro and in vivo. Taken together, our results suggest that ROR1 is a novel prognostic marker for DLBCL survival and ROR1 significantly promotes DLBCL tumorigenesis by regulating the PI3K/Akt/mTOR signaling pathway. Targeting ROR1 may provide a promising strategy for DLBCL treatment. © 2019 BioFactors, 45(3):416–426, 2019
Transcriptomic analysis of IgG4 Fc‐fusion protein degradation in a panel of clonally‐derived CHO cell lines using RNASeq
Authors: Clarke Colin , Gallagher Clair , Kelly Ronan M. , Henry Michael , Meleady Paula , Frye Christopher C. , Osborne Matthew D. , Brady Ciaran P , Barron Niall , Clynes Martin 
Published: February 2019

AbstractIn this study, we report an investigation of a panel of clonally‐derived Chinese hamster ovary (CHO) cell lines exhibiting variability in the proportion of full‐length IgG4 Fc‐fusion protein produced. The recombinant protein was found to be degraded during cell culture into four shorter “clipped” species (three of the four cleavage sites occurred at arginine residues) and preliminary analyses suggested that a host cell enzyme was responsible for proteolysis. To identify the specific enzyme responsible, RNA sequencing was used to identify gene expression differences between the cell lines with a “high” and “low” clipping phenotype. From this analysis, six protease‐encoding genes were found to be significantly upregulated in those cell lines yielding the lowest proportion of full‐length IgG4 Fc‐fusion protein. Four of these protease candidates were deprioritized after examination of their cleavage site specificity. The remaining enzymes, Adam19 and Furin, were found to be capable of cleavage at arginine residues, and inhibitors for both proteases were added to cell‐free media to determine if the product degradation could be reduced. While the Adam19 inhibitor had no impact, Furin inhibitor I (specific for the proprotein convertase family of enzymes) was found to result in a 33–39% increase in complete IgG4 Fc‐fusion protein when compared with untreated samples.
Three‐dimensional microfluidic tumor–macrophage system for breast cancer cell invasion
Authors: Mi Shengli , Liu Zhaoyu , Du Zhichang , Yi Xiaoman , Sun Wei 
Published: February 2019

AbstractThe recrudescence of breast cancer can partly be attributed to poor understanding of the early steps and the mechanisms involved in breast cancer metastasis, especially how tumor inflammatory cells including tumor‐associated macrophages (TAM) affect invasion process. However, invasion‐related biological studies in traditional in vitro assays or in vivo models are challenging due to the arduousness in establishing models that precisely reproduce the tumor invasion environment. To this end, we proposed a juxtaposed dual‐layer cell‐loaded hydrogels biomimetic microfluidic system and formed monolayer size‐selective permeable vascular endothelial barriers besides the dual layer to mimic mammalian blood vessels. We clarified that in this system, TAM promoted the invasion of breast cancer cells, whereas breast cancer cells maintained the phenotype of TAM cells and promoted the differentiation of U937 cells into TAM. It formed a tumor–macrophage bidirectional crosstalk system. This system could be used for drug screening. So finally, through the calculation of the survival rate of breast cancer cells when cocultured with different macrophages under paclitaxel treatment, we analyzed the antagonism of tumor–macrophage bidirectional crosstalk on anticancer drugs.
Ovicidal and Deleterious Effects of Cashew (Anacardium occidentale) Nut Shell Oil and Its Fractions on Musca domestica, Chrysomya megacephala, Anticarsia gemmatalis and Spodoptera frugiperda
Authors: Ferreira de Carvalho George Harrison , Lucília dos Santos Maria , Monnerat Rose , Aparecida Andrade Milene , Gonçalves de Andrade Marina , Barbosa dos Santos Alessandra , Marques Dourado Bastos Izabela , de Santana Jaime Martins 
Published: February 2019

AbstractIn this work, we evaluated the ovicidal activity and the deleterious effects of cashew (Anacardium occidentale) nut shell oil and its fractions on the development of Musca domestica and Chrysomya megacephala, important vectors of several diseases. The insecticidal effects of this plant were also measured on the first and second instar larvae of Anticarsia gemmatalis and Spodoptera frugiperda, soy and maize pests, respectively. The fly eggs and the crop pest insect larvae were exposed to the cashew (Anacardium occidentale) nut shell liquid (CNSL) and its fractions: technical CNSL, anacardic acid, cardanol and cardol. The results show that the cardol fraction, for both species of flies, presented the lowest lethal concentration with LC50 of 80.4 mg/L for M. domestica and 90.2 mg/L for C. megacephala. For the mortality of the larvae of A. gemmatalis and S. frugiperda, the most effective fraction was anacardic acid with LC50 of 295.1 mg/L and 318.4 mg/L, respectively. In all species, the mortality rate of the commercial compounds (cypermethrin 600 mg/L and temephos 2 mg/L) was higher than that of the evaluated compounds. Despite this, the results obtained suggest their potential in field trials, once the fractions of A. occidentale presented high mortality at low lethal concentrations in laboratory conditions, with the possibility of integrated use in the control of disease vectors and agricultural pests, employing ecofriendly compounds.
Coupled Enzymatic Treatment and Mass Spectrometric Analysis for Identification of Glucuronidated Metabolites in Human Samples
Authors: Correia Mario S. P. , Rao Menghua , Ballet Caroline , Globisch Daniel 
Published: February 2019

AbstractGlucuronidation is the most common phase II modification and plays an important role in human clearance metabolism. Glucuronidated metabolites have also been linked to disease development and microbiota–host co‐metabolism. Although many of these compounds have been identified, the total number of unknown glucuronides and their impact on the human host's physiology can only be estimated. Herein, we describe the combination of an untargeted metabolomics analysis and enzymatic metabolic conversion for the selective detection of glucuronide conjugates by using UPLC‐MS/MS in human urine samples. Our study demonstrates that this powerful strategy can be used for the selective identification of glucuronidated molecules and to discover unknown natural metabolites. In total, we identified 191 metabolites in a single sample including microbiota‐derived compounds as well as previously unidentified molecules.
2nd PSL Chemical Biology Symposium (2019): At the Crossroads of Chemistry and Biology
Authors: Lucchino Marco , Billet Anne , Versini Antoine , Bavireddi Harikrishna , Dasari Bhanu‐Das , Debieu Sylvain , Colombeau Ludovic , Cañeque Tatiana , Wagner Alain , Masson Géraldine , Taran Frédéric , Karoyan Philippe , Delepierre Muriel , Gaillet Christine , Houdusse Anne , Britton Sébastien , Schmidt Frédéric , Florent Jean‐Claude , Belmont Philippe , Monchaud David , Cossy Janine , Thomas Christophe , Gautier Arnaud , Johannes Ludger , Rodriguez Raphaël 
Published: February 2019

AbstractChemical Biology is the science of designing chemical tools to dissect and manipulate biology at different scales. It provides the fertile ground from which to address important problems of our society, such as human health and environment.
Distal transradial artery access in the anatomical snuffbox for coronary angiography as an alternative access site for faster hemostasis
Authors: Aoi Shunsuke , Htun Wah Wah , Freeo Stephanie , Lee Samuel , Kyaw Htoo , Alfaro Victor , Coppola John , Pancholy Samir , Kwan Tak 
Published: February 2019

AbstractObjectivesThis study investigated the feasibility, safety, and the potential benefit of faster hemostasis with the distal transradial artery access (TRA).BackgroundTRA has been shown to be associated with lower bleeding and vascular complications. Limited data are available regarding the new technique of accessing the distal radial artery in the anatomical snuffbox.MethodsWe retrospectively reviewed 202 consecutive patients who underwent coronary angiography and intervention with distal TRA. Two hundred and six conventional TRA cases were collected as a comparison arm.ResultsOut of 408 patients, successful distal radial access was obtained in 99.5% (201/202) in the distal TRA cases and 99.0% (204/206) in the conventional TRA cases. The rate of access site crossover was 2.0% (4/202) for distal TRA. Right distal radial artery was accessed in 176 cases (87.6%). Mean access time from local anesthesia to radial flush was 7.3 min. Ninety cases (44.8%) were percutaneous coronary interventions (PCIs) and the mean heparin dose used was 4,448 units (6,009 units for PCI and 3,182 units for diagnostic catheterization). Mean time to remove TR band was 104.7 min (120.8 min for PCI and 91.7 min for diagnostics). Follow‐up ultrasound study showed two partial occlusions (1.0%) and one arteriovenous fistula (0.5%) that resolved with prolonged TR band inflation.ConclusionsDespite longer time to access the distal radial artery in the anatomical snuffbox, it is a safe and feasible alternative to conventional TRA and might result in shorter time to hemostasis especially in cases of PCI.
Primary cutaneous CD4+ small‐/medium‐sized T‐cell lymphoproliferative disorder: A case report
Authors: Koper Monika , Putała‐Pośpiech Małgorzata , Biernat Wojciech , Woźniacka Anna , Robak Ewa 
Published: February 2019

Key Clinical MessagePrimary cutaneous CD4+ small‐/medium‐sized T‐cell lymphoproliferative disorder is usually characterized by nodules and plaques affecting the upper part of the body. The present case presented with a large, single tumor located on a lower extremity. The patient did not respond to surgical therapy but responded to cyclophosphamide, methotrexate, and radiotherapy.
Enzyme‐Catalyzed Synthesis of Esters in Water
Authors: Mestrom Luuk , Claessen Jord G. R. , Hanefeld Ulf 
Published: February 2019

AbstractMsAcT catalyzes the esterification of primary alcohols in water. When utilizing acid and alcohol as starting materials low yields dictated by thermodynamics were observed. However, with activated esters such as ethyl acetate and vinyl acetate very high yields of the desired ester can be achieved in combination with the appropriate alcohol. This study investigated both the intrinsic kinetic properties of MsAcT for the hydrolysis and transesterification of esters in water as well as the thermodynamics of the reaction. In comparison to the chemical or enzymatic ester synthesis using either toxic reagent, and harsh organic solvents, the MsAcT‐catalyzed synthesis of esters of primary alcohols can be achieved efficiently in water without neutralization steps.
Catalysis and Stability Effect of Solvent Alcohol on the C6 Aldose Conversion toward Tetrose
Authors: Hou Wenrong , Yan Yueer , Li Gang , Zhan Yulu , Feng Lei , Zhang Ruohong , Hua Li Zhen , Zhang Yahong , Tang Yi 
Published: February 2019

AbstractConversions of biomass feedstock into various valuable chemicals are of great significance. As a typical route, retro‐aldol condensation of monosaccharide greatly expands the variety of biomass‐derived platform chemicals via a selective C−C splitting. Herein, we describe a solvent‐catalysed strategy to high‐selectively accumulate tetrose (four‐carbon platform chemical) from C6 aldoses via the retro‐aldol/aldol process. We find that alcohol solvents with Lewis acidity facilitate the C−C splitting process of hexose under the catalyst‐free condition. The conversion is the fastest in methanol while it is the slowest in isopropanol. The product distribution is greatly influenced by the alcohols through shifting the equilibrium between tetrose and glycolaldehyde (GA). The addition of catalyst only accelerates the reaction rate, and does not change the product distribution. On the one hand, the acetalization of GA with methanol or ethanol shifts the equilibrium from tetrose toward GA, which results in a low yield of tetrose in methanol or ethanol solvent. On the other hand, tetrose can be well accumulated in isopropanol or n‐butanol, and the yield of tetrose in isopropanol is higher than in n‐butanol because tetrose can be well solvated and stabilized in it. This solvent‐dependent reaction strategy provides a new possibility which contributes to the conversion of biomass feedback into valuable platform chemicals and accumulation of target products by utilizing the solvation effect.
Chloramphenicol Base: A New Privileged Chiral Scaffold in Asymmetric Catalysis
Authors: Xiao You‐Cai , Chen Fen‐Er 
Published: February 2019

AbstractThis Minireview focuses on the synthetic application of a chloramphenicol base (ANP)‐derived catalyst. In the illustrated examples, ANP derivatives have proven to be readily available and highly versatile catalysts in a wide range of asymmetric transformations, showing high proficiency as both chiral ligands (amino alcohol, Schiff base and oxazoline‐containing ligand) and organocatalyst (hydrogen bonding‐based bifunctional catalyst and phase transfer catalyst).
Modification of a Poly(tetrafluoroethylene) Porous Membrane to Superhydrophilicity with Improved Durability
Authors: Liu Shijie , Cui Suping , Qin Zhenping , Zhang Xuehong , Zhao Yao , Zhao Yingying , Guo Hongxia 
Published: February 2019

AbstractThe commercial fluorocarbon surfactant (FCS) DuPont™ Zonyl 321 fluorinated cationic surfactants, was employed to modify both flat‐sheet and hollow‐fiber poly(tetrafluoroethylene) (PTFE) porous membranes. The variation of morphology, wettability, and hydrophilicity durability to acid and alkali as well as the filtration performance of both membranes were investigated. Such superhydrophilic membranes showed better wetting stability and higher resistance to acid and alkali solutions. Moreover, both membranes exhibited good filtration performance.
Mixing of Oil in Water Through Electrical Resistance Tomography and Response Surface Methodology
Authors: Mirshekari Fahimeh , Pakzad Leila 
Published: February 2019

AbstractThe mixing performance of the oil‐in‐water dispersion system was evaluated. Using an electrical resistance tomography system composed of two measuring planes, the effect of parameters such as impeller type, impeller speed, oil type, and oil volume fraction on the mixing performance through axial mixing indices were explored. The oil type and the oil volume fraction were identified as the most influential factors on the mixing index. Castor oil, with the highest viscosity of the tested oils, was found as the most difficult oil to disperse. The Scaba impeller was the most efficient impeller in dispersing oil in water. The interactions between oil type and impeller type as well as between impeller speed and oil type, had the greatest impact on the mixing index.