bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Microorganisms Associated with the Quality Improvement of Dry‐Aged Beef Through Microbiome Analysis and DNA Sequencing, and Evaluation of Their Effects on Beef Quality
Authors: Oh Hyemin , Lee Hyun Jung , Lee Jiyoung , Jo Cheorun , Yoon Yohan 
Published: September 2019

AbstractThe objective of this study was to isolate and identify the microorganisms, especially yeasts and molds, related to the improvement of beef quality during dry‐aging of beef through microbiome analysis, and to examine the possibility of using them as starter culture strains to improve the efficiency of dry‐aging beef production. Beef sirloins were dry‐aged for 28 days using different wind speeds (0, 2.5, and 5 m/s) at 1 to 3 °C and 75% relative humidity, and microbial compositions were confirmed by microbiome analysis. Mold and yeast samples were plated on potato dextrose agar supplemented with 10% tartaric acid, and the isolated colonies were identified by DNA sequencing. The isolates were subjected to microbial characterization (morphological characterization, growth condition, and enzyme activity). Microbiome analysis showed that the dominant microorganisms were molds and yeasts identified as Pilaira anomala SMFM201611 and Debaryomyces hansenii SMFM201707. Pilaira anomala SMFM201611 and D. hansenii SMFM201707 were inoculated into 24 sirloins of the lowest grade. All samples were dry‐aged for 0, 14, 21, and 28 days and analyzed for microbial growth, pH, shear force, ultrastructure, and flavor compounds (free amino acids and free fatty acids). Inoculation with P. anomala SMFM201611 and D. hansenii SMFM201707 improved tenderness and cause the breakdown of myofibrils by proteolysis. Both microorganisms also produced free amino acids and fatty acids through proteolytic and lipolytic activities. These results indicate that P. anomala SMFM201611 and D. hansenii SMFM201707 isolated and identified from dry‐aged beef can improve the quality of low‐grade beef during dry‐aging.Practical ApplicationDuring dry‐aging, mold and yeast improve the quality of dry‐aged beef. Pilaira anomala SMFM201611 and Debaryomyces hansenii SMFM201707 isolated from dry‐aged beef can improve tenderness by breaking down myofibrils. Both microorganisms improve flavor by producing free fatty acids and amino acids, and the taste and aroma characteristics of low‐grade beef may be improved during the dry‐aging process.
Fatigue in complete spinal cord injury and implications on total delay
Authors: Rinaldin Carla Daniele Pacheco , Cabral Luciane Patrícia Adreani , Krueger Eddy , Nogueira‐Neto Guilherme N. , Nohama Percy , Scheeren Eduardo M. 
Published: September 2019

AbstractThe use of neuromuscular electrical stimulation (NMES) to artificially restore movement in people with complete spinal cord injury (SCI) induces an accelerated process of muscle fatigue. Fatigue increases the time between the beginning of NMES and the onset of muscle force (DelayTOT). Understanding how much muscle fatigue affects the DelayTOT in people with SCI could help in the design of closed‐loop neuroprostheses that compensate for this delay, thus making the control system more stable. The aim of this study was to evaluate the impact of the extent of fatigue on DelayTOT and peak force of the lower limbs in people with complete SCI. Fifteen men—young adults with complete SCI (paraplegia and tetraplegia) and stable health—participated in the experiment. DelayTOT was defined as the time interval between the beginning of NMES application until the onset of muscle force. The electrical intensity of NMES applied was adjusted individually and consisted of the amplitude required to obtain a full extension of the knee (0°), considering the maximum electrically stimulated extension (MESE). Subsequently, 70% of the MESE was applied during the fatigue induction protocol. Significant differences were identified between the moments before and after the fatigue protocol, both for peak force (P ≤ .026) and DelayTOT (P ≤ .001). The medians and interquartile range of the DelayTOT were higher in postfatigue (199.0 ms) when compared to the moment before fatigue (146.5 ms). The medians and interquartile range of the peak force were higher in unfatigued lower limbs (0.43 kgf) when compared to the moment postfatigue (0.27 kgf). The results support the hypothesis that muscle fatigue influences the increase in DelayTOT and decrease in force production in people with SCI. For future applications, the combined evaluation of the delay and force in SCI patients provides valuable feedback for NMES paradigms. The study will provide potentially critical muscle mechanical evidence for the investigation of the evolution of atrophy.
Spatio‐temporal models of intermediate complexity for ecosystem assessments: A new tool for spatial fisheries management
Authors: Thorson James T. , Adams Grant , Holsman Kirstin 
Published: September 2019

AbstractMultispecies models are widely used to evaluate management trade‐offs arising from species interactions. However, identifying climate impacts and sensitive habitats requires integrating spatial heterogeneity and environmental impacts into multispecies models at fine spatial scales. We therefore develop a spatio‐temporal model of intermediate complexity for ecosystem assessments (a “MICE‐in‐space”), which is fitted to survey sampling data and time series of fishing mortality using maximum‐likelihood techniques. The model is implemented in the VAST R package, and it can be configured to range from purely descriptive to including ratio‐dependent interactions among species. We demonstrate this model using data for four groundfishes in the Gulf of Alaska using data from 1982 to 2015. Model selection for this case‐study shows that models with species interactions are parsimonious, although a model specifying separate density dependence without interactions also has substantial support. The AIC‐selected model estimates a significant, negative impact of Alaska pollock (Gadus chalcogrammus, Gadidae) on productivity of other species and suggests that recent fishing mortality for Pacific cod (G. microcephalus, Gadidae) is above the biological reference point (BRP) resulting in 40% of unfished biomass; other models show similar trends but different scales due to different BRP estimates. A simulation experiment shows that fitting a model with fewer species at a coarse spatial resolution degrades estimation performance, but that interactions and biological reference points can still be estimated accurately. We conclude that MICE‐in‐space models can simultaneously estimate fishing impacts, species trade‐offs, biological reference points and habitat quality. They are therefore suitable to forecast short‐term climate impacts, optimize survey designs and designate protected habitats.
Ciliogenesis‐coupled accumulation of IFT‐B proteins in a novel cytoplasmic compartment
Authors: Lamri Lynda , Twan Wang Kyaw , Katoh Takanobu A. , Botilde Yanick , Takaoka Katsuyoshi , Ikawa Yayoi , Nishimura Hiromi , Fukumoto Akemi , Minegishi Katsura , Mizuno Katsutoshi , Hamada Hiroshi 
Published: September 2019

AbstractCluap1/IFT38 is a ciliary protein that belongs to the IFT‐B complex and is required for ciliogenesis. In this study, we have examined the behaviors of Cluap1 protein in nonciliated and ciliated cells. In proliferating cells, Cluap1 is located at the distal appendage of the mother centriole. When cells are induced to form cilia, Cluap1 is found in a novel noncentriolar compartment, the cytoplasmic IFT spot, which mainly exists once in a cell. Other IFT‐B proteins such as IFT46 and IFT88 are colocalized in this spot. The cytoplasmic IFT spot is present in mouse embryonic fibroblasts (MEFs) but is absent in ciliogenesis‐defective MEFs lacking Cluap1, Kif3a or Odf2. The cytoplasmic IFT spot is also found in mouse embryos but is absent in the Cluap1 mutant embryo. When MEFs are induced to form cilia, the cytoplasmic IFT spot appears at an early step of ciliogenesis but starts to disappear when ciliogenesis is mostly completed. These results suggest that IFT‐B proteins such as Cluap1 accumulate in a previously undescribed cytoplasmic compartment during ciliogenesis.
Research Tracker 6 Accelerometer Calibration and Validation in Comparison to GENEActiv, ActiGraph, and Gas Analysis in Young Adults
Authors: Eyre Emma L. J. , Tallis Jason , Wilson Susie , Wilde Lee , Akhurst Liam , Wanderleys Rildo , Duncan Michael J. 
Published: September 2019

Background: The ability to objectively assess physical activity and inactivity in free living individuals is important in understanding activity patterns and the dose response relationship with health. Currently, a large number of research tools exist, but little evidence has examined the validity/utility of the Research Tracker 6 (RT6) monitor. Questions remain in regard to the best placements, positions, and cut-points in young adults to determine activity intensity across a range of activities. This study sought to address this gap in young adults. The study aims were 1) to examine criterion validity of RT6 in comparison to breath-by-breath gas analysis; 2) convergent validity of RT6 in comparison to ActiGraph and GENEActiv; 3) development of RT6 tri-axial vector magnitude cut-points to classify physical activity at different intensities (i.e., for sedentary, moderate, and vigorous); 4) to compare the generated cut-points of the RT6 in comparison to other tools. Methods: Following ethics approval and informed consent, 31 young adults (age = 22±3 years: BMI = 23±3 kg/m2) undertook five modes of physical activity/sedentary behaviors while wearing three different accelerometers at hip and wrist locations (ActiGraph GT9X Link, GENEActiv, RT6). Expired gas was sampled during the five activities (MetaMax 3B). Correlational analysis assessed the relationship between accelerometer devices and METs/VO2. Receiver Operating Characteristic Curves analysis were used to calculate area under the curve and define cut-points for physical activity intensities. Results: The RT6 demonstrated criterion and convergent validity (r = 0.662–0.966, P < .05). RT6 generally performed good to excellent across activity intensities and monitor position (sedentary [AUC = 0.862–0.911], moderate [AUC = 0.849–0.830], vigorous [AUC = 0.872–0.877]) for non-dominant and dominant position, respectively. Cut-points were derived across activity intensities for non-dominant- and dominant-worn RT6 devices. Comparison of the RT6 derived cut-points identified appropriate agreement with comparative tools but yields the strongest agreement with the ActiGraph monitor at the hip location during sedentary, light, and moderate activity. Conclusion: The RT6 performed similar to the ActiGraph and GENEActiv and is capable of classifying the intensity of physical activity in young adults. As such this may offer a more useable tool for understanding current physical activity levels and in intervention studies to monitor and track changes without the excessive need for downloading and making complex analysis, especially given the option to view energy expenditure data while wearing it. The RT6 should be placed on the dominant hip when determining activities that are sedentary, moderate, or vigorous intensity.
A Yilgarn seed to the Pilbara Craton (Australia)? Evidence from inherited zircons
Authors: Petersson Andreas , Kemp Anthony I.S. , Whitehouse Martin J. 
Published: September 2019

Abstract
Knowledge of the age and compositional architecture of Archean cratonic lithosphere is critical for models of geodynamics and continental growth on early Earth, but can be difficult to unravel from the exposed geology. We report the occurrence of numerous >3.7 Ga zircon crystals in 3.45 Ga rhyolites of the eastern Pilbara Craton (Western Australia), which preserve evidence for an Eoarchean meta-igneous component in the deep Pilbara crust. This inherited zircon population shares similar and distinctive age and Hf-O isotope characteristics with the oldest gneissic components of the Yilgarn Craton ∼500 km farther south, suggesting a common ca. 3.75 Ga felsic crustal nucleus to these two Archean granite-greenstone terranes. We infer a pivotal role for such ‘seeds’ in facilitating the growth and persistence of Archean continental lithosphere.
Clinical Outcomes Before and After Complete Everolimus-Eluting Bioresorbable Scaffold Resorption
Authors: Kereiakes Dean J. , Ellis Stephen G. , Metzger D. Christopher , Caputo Ronald P. , Rizik David G. , Teirstein Paul S. , Litt Marc R. , Kini Annapoorna , Kabour Ameer , Marx Steven O. , Popma Jeffrey J. , Tan Siok Hwee , Ediebah Divine E. , Simonton Charles , Stone Gregg W. ,   For the ABSORB III Investigators
Published: September 2019

Background:
The Absorb everolimus-eluting bioresorbable vascular scaffold (BVS) provides early drug delivery and mechanical support similar to those of metallic drug-eluting stents, followed by complete resorption in ≈3 years with recovery of vascular structure and function. The ABSORB III trial demonstrated noninferior rates of target lesion failure (cardiac death, target vessel myocardial infarction, or ischemia-driven target lesion revascularization) at 1 year with BVS compared with cobalt chromium everolimus-eluting stents. Between 1 and 3 years and cumulative to 3 years, adverse event rates (particularly target vessel myocardial infarction and scaffold thrombosis) were increased after BVS. We sought to assess clinical outcomes after BVS through 5 years, including beyond the 3-year time point of complete scaffold resorption.

Methods:
Clinical outcomes from ABSORB III were analyzed by randomized device (intention to treat) cumulative to 5 years and between 3 and 5 years.

Results:

Rates of target lesion failure, target vessel myocardial infarction, and scaffold thrombosis were increased through the 5-year follow-up with BVS compared with everolimus-eluting stents. However, between 3 and 5 years, reductions in the relative hazards of the BVS compared with everolimus-eluting stents were observed, particularly for target lesion failure (hazard ratio, 0.83 [95% CI, 0.55–1.24] versus 1.35 [95% CI, 1.02–1.78];
P
int
=0.052) and scaffold thrombosis (hazard ratio, 0.26 [95% CI, 0.02–2.87] versus 3.23 [95% CI, 1.25–8.30];
P
int
=0.056) compared with the 0- to 3-year time period.

Conclusions:
In the ABSORB III trial, cumulative 5-year adverse event rates were increased after BVS compared with everolimus-eluting stents. However, the period of excess risk for BVS ended at 3 years, coincident with complete scaffold resorption.

Clinical Trial Registration:

URL:
https://clinicaltrials.gov
. Unique identifier: NCT01751906.
Treatment of posttraumatic headache migraine phenotype with erenumab – An observational study
Authors: Charles James A 
Published: September 2019

Objective To report the observed effect of erenumab in mild posttraumatic headache migraine phenotype with and without aura. Background There is no clinical algorithm of pharmacotherapy for migraine following posttraumatic headache. Most migraine preventatives that are typically used are either ineffective or not tolerated. Methods Seven patients who met the clinical criteria for migraine with or without aura following posttraumatic headache who failed or were intolerant of conventional migraine preventatives were treated with erenumab 140 mg subcutaneously. Most had no history of migraine. In those patients with a history of migraine, the posttraumatic headache migraine headaches were different than the past migraine experience. Descriptive headache intensity or disability using the Head Impact Test-6 and monthly headache days were recorded before and after treatment. All patients were debilitated on presentation and demonstrated no signs of spontaneous resolution. Results Patients responded with a 95% (SD 1.22, p < .001) reduction in headache days. All Head Impact Test-6 scores went from disabling to non-disabling without adverse effects. Most required only one dose of erenumab with no migraine recurrence. Onset of efficacy often became apparent within days to four weeks. Extended follow-up six months after treatment revealed no relapses. Conclusions Erenumab is effective in the treatment of posttraumatic headache with migraine phenotype in this small cohort. Large-scale studies are urgently required for this highly prevalent, disabling, condition which has no effective established treatment.
EVALUATION OF UVEITIS ACTIVITY USING BOS24 IN PATIENTS WITH BEHСET'S DISEASE
Authors: Lisitsyna T. A. , Davydova G. A. , Khatagova Z. R. , Katargina L. A. , Alekberova Z. S. 
Published: September 2019

Objective:to estimate changes of uveitis activity using BOS24 (Behсet's disease Ocular attack Score 24) during antiinflammatory and immunosuppressive therapy in patients with Behcet's disease (BD).Subjects and methods.103 (75.6%) of the 138 patients with BD fulfilled the 1990 International Study Group for Behсet's Disease (ISGBD) criteria had eye lesions; 55 (53.4%) of the 103 patients had an exacerbation of uveitis. 55 patients with an exacerbation of uveitis were found to have active inflammation in 94 eyes. The activity of uveitis was monitored during anti-inflammatory and immunosuppressive therapy, by using BOS24 that consists of 6 parameters with maximal possible value 24.Results and discussion.The mean BOS24 for 94 eyes with active uveitis at baseline was 9.33±0.91. The most pronounced inflammatory changes were found in the posterior chamber of the eye, mainly in the area of the peripheral retina, rarely in the area of the fovea and in the optic disc. All the patients with an exacerbation of uveitis received systemic therapy with glucocorticoids, cyclosporine and/or azathioprine. After 8.92±3.47 months of treatment, the mean BOS2 decreased significantly (p < 0.001) to 2.20±1.02. The most substantial positive changes were noted in the anterior chamber of the eye (p = 0.03), vitreous humor (p < 0.01), and peripheral retina (p < 0.001).Conclusion. BOS24 is a reliable tool to quantify uveitis activity in patients with BD and its dynamics during antiinflammatory and immunosuppressive therapy.
PAIN AND INFLAMMATION. PART 1. PATHOGENETIC ASPECTS
Authors: Karateev A. E. , Karateev D. E. , Davydov O. S. 
Published: September 2019

The relief of suffering, which is associated with a rapid and complete elimination of painful sensations, is the most important challenge facing physicians of many specialties. It is obvious that it can be solved only when you understand clearly the processes governing the development and chronization of pain. Inflammation, a universal adaptive mechanism that always accompanies damage to living tissues, plays a key role. Part 1 of this review considers the main stages of development of an inflammatory response, beginning with primary damage accompanied by the release of molecules acting as an alarm and ending with the deployment of a complete picture of the inflammatory response with the involvement of many cell elements and the overexpression of cytokines and proinflammatory mediators. The biological basis of the peripheral and central nociceptive sensitization phenomenon that is rigidly associated with inflammation is presented. Particular emphasis is placed on the possible natural completion of the inflammatory response, on the adaptive mechanisms regulating this process and on the reasons that prevent this and determines inflammation chronization.
A preliminary assessment of potential ecological risk and soil contamination by heavy metals around a cement factory, western Saudi Arabia
Authors: El-Sherbiny Mohsen M. , Ismail Ali I. , EL-Hefnawy Mohamed E. 
Published: September 2019

Abstract
Twenty surface soil samples (0-10 cm) and shoots of a perennial shrub Zygophyllum coccineum L. were collected around a cement factory on the western coast of Saudi Arabia, in order to assess concentrations of some heavy metals (Cr, Cu, Ni, Pb and Zn). The most noticeable among all heavy metals was Pb that showed an average concentration of 460.15±86.60 μg g-1 followed by Cr (138.67±30.89 μg g-1), Zn (54.41±43.79 μg g-1), Ni (41.22±12.60 μg g-1) and Cu (33.48±12.52 μg g-1). Based on biological concentration factor analysis, Z. coccineum can be considered as an accumulator only for zinc (BCF >1). Estimation of various ecological contamination factors revealed the significant impact of Pb in the environmental pollution in the region. It is also understood that the primary contribution to the ecological risk index (RI) mainly originated from various anthropogenic influences such as industrialization and urbanization. The different statistical analysis further revealed the potential effect of soil characteristics on the occurrence and dispersal of heavy metals in the study area.
Tumor-suppressive activity of sTRAIL on circulating CD44+ cells in patients with non-small cell lung cancer
Authors: Sun Yan-Bin , Sun Guang-Hao , Xu Shun , Xu Jing-Jing 
Published: September 2019

Abstract
Circulating CD44+ cells have been identified as a prognostic marker for patients with non-small cell lung cancer (NSCLC). Serum tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL) is involved in the pathophysiology of many cancers. However, no previous studies have shown the roles of sTRAIL in circulating CD44+ cells in the blood of NSCLC patients. We detected circulating CD44+ cells and sTRAIL levels in blood samples from NSCLC patients using flow cytometry and an enzyme-linked immunosorbent assay (ELISA). Anti-tumor roles of TRAIL in CD44+ cells were confirmed using a CCK-8 assay and mouse models. A higher number of circulating CD44+ cells were identified in NSCLC patients compared with healthy control individuals. In addition, we confirmed the anti-tumor roles and mechanisms of TRAIL in CD44+ cells both in vitro and in vivo. Our results indicate that (1) there is a negative correlation between sTRAIL and circulating CD44+ cells in NSCLC patients and (2) CD44+ cells have cancer stem cell properties and are more sensitive than CD44− cells to TRAIL.
ESTUDO DE PATOLOGIAS EM ESTRUTURAS METÁLICAS E O IMPACTO AMBIENTAL NA PRODUÇÃO DO MATERIAL
Authors: Dos Reis Moreira Eloan Marlon , De Farias Bruno Matos , Souza da Silva Murilo Henrique , Ferreira de Oliveira Bruno Ricardo , Portela Fellipe De Souza , Sousa dos Santos Hagattacley Brum 
Published: September 2019

A indústria siderúrgica é a maior indústria de manufatura de consumo de energia do mundo, com a maior participação na economia mundial. Na produção de ferro e aço no mundo, a China ocupa o primeiro lugar e o Japão e os EUA a seguem. No meio acadêmico há diversas pesquisas quando o assunto é patologia das estruturas, porém na maioria dos casos, o modelo de edificação adotado no estudo, é aquela na qual são construídas em concreto armado ou protendido. E quando o assunto é estruturas de madeira, se encontra menos ênfase do que as estruturas de concreto, e por fim, estudos de patologias em estruturas de aço, esta última por se tratar de um material com características e de natureza muito diferente da madeira e do concreto. Neste caso, a maioria dos problemas aparecem quando a utilização é mais específica, sendo inegável saber tratar com problemas dessas estruturas a fim de conseguir manter seu desempenho por toda a sua vida útil. O objetivo deste artigo é apresentar alguns quantitativos de problemas de patologias em algumas construções em aço. Procurou-se ainda determinar respostas apresentando processos de reforço, reparos e manutenção, gerando então fatores para se evitar e controlar as causas dos problemas. O estudo se limita apenas às questões de impacto ambiental devido a produção do aço e as patologias nas estruturas metálicas.