bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paeonol Alleviates Subarachnoid Hemorrhage Injury in Rats Through Upregulation of SIRT1 and Inhibition of HMGB1/TLR4/MyD88/NF‐κB Pathway
Authors: Liu Zhao , Zhu Jun , Pan Enyu , Pang Lujun , Zhou Xiwei , Che Yanjun 
Published: November 2024

ABSTRACTPaeonol is a principle bioactive compound separated from the root bark of Cortex Moutan and has been shown to confer various biological functions, including antineuroinflammation and neuroprotection. Inflammation, blood–brain barrier (BBB), permeability, and apoptosis are three major underlying mechanisms involved in early brain injury (EBI) postsubarachnoid hemorrhage (SAH). This study aimed to detect the roles and mechanisms of paeonol in EBI following SAH. A SAH model was established by an endovascular perforation method in Sprague‐Dawley rats. The localizations of HMGB1 and p65 were identified by immunofluorescence staining. Protein levels were measured by western blot analysis. The serum levels of HMGB1 and the levels of inflammatory cytokines in the brain cortex were evaluated by ELISA. Hematoxylin and eosin staining was conducted to detect neuronal degeneration. Brain water content and Evans blue extravasation were assessed to determine EBI. Neuronal apoptosis was examined by TUNEL. Paeonol deacetylated HMGB1 by upregulating SIRT1 level. SIRT1 inhibition attenuated the protective effects of paeonol against neurological dysfunctions, brain edema, and BBB disruption. SIRT1 inhibition rescued the paeonol‐induced inhibition in inflammatory response. The paeonol‐induced decrease in neuronal apoptosis was restored by SIRT1 inhibitor. The paeonol‐mediated deactivated TLR4/MyD88/NF‐κB pathway was activated by SIRT1 inhibitor. Paeonol alleviates the SAH injury in rats by upregulating SIRT1 to inactivate the HMGB1/TLR4/MyD88/NF‐κB pathway.
Plausible Action of N‐(3,4‐Dimethoxy‐Phenyl)‐6,7‐Dimethoxyquinazoline‐4‐Amine (TKM01) as an Armor Against Alzheimer's Disease: In Silico and In Vivo Insights
Authors: Kashif Mohd , Chandrabose Karthikeyan , Pandurangan Ashok Kumar 
Published: November 2024

ABSTRACTAlzheimer's disease (AD) affects millions of people and has limited treatment options, thus making it a global health concern. Amyloid β (Aβ), a disrupted cholinergic system with high acetylcholinesterase (AChE), oxidative stress (OS), reduced antioxidants, and neuroinflammation are key factors influencing AD progression. Prior research has shown that AChE can interact with Aβ and increase its accumulation and neurotoxicity, so targeting AChEs and Aβ could be a potential therapeutic approach for AD treatment. It has been known that nonsteroidal anti‐inflammatory drugs (NSAIDs) can inhibit Aβ accumulation. Previously, TKM01, a derivative of 4‐anilinoquinazoline, has demonstrated inhibitory effects against GSK‐3β—a regulator in AD progression. The current research included molecular docking studies of NSAIDs and TKM01 with Aβ and AChEs as targets. TKM01 exhibited a higher binding affinity with Aβ among all tested compounds. Molecular dynamic (MD) simulations confirmed the stability of the protein‐TKM01 complexes. TKM01 also exhibited favorable drug‐likeness properties, and no hepatoxicity was visualized in comparison with other compounds. Further, in vitro assay showed an inhibitory action of TKM01 (50–1200 µg/mL) on AChEs. In the in vivo studies on zebrafish larvae brains, we found that TKM01 (120 and 240 µg/mL) reduced the levels of AChEs and lipid peroxidation (LPO) and increased antioxidant superoxide dismutase (SOD) and catalase (CAT) in AlCl3(80 µM)‐induced AD‐like model. Additionally, TKM01 treatment was found to decrease pro‐inflammatory cytokines TNF‐α, IL‐1β, and IL‐6. The current study demonstrates that TKM01 can be used to treat AD. Nonetheless, experimental validation is needed to reveal the cellular, sub‐cellular, and molecular mechanisms and possible implications at a clinical stage.
Exploring the Molecular Interaction Between NR2E3 and NR1D1 in Retinitis Pigmentosa: A Docking and Molecular Dynamics Study
Authors: Vafaeie Farzane , Mohammadpour Mojtaba , Etesam Shokoofeh , Zarifi Shahnaz , Yari Abolfazl , Nikandish Malihe , Hashemzadeh Hassan , Hajiabadi Mohammad Reza , Miri‐Moghaddam Ebrahim 
Published: November 2024

ABSTRACTBackground and AimsRetinitis pigmentosa (RP) is a hereditary retinal disorder that gradually leads to vision loss due to photoreceptor cell degeneration. This study aims to investigate the clinical features and genetic underpinnings of RP within a large Iranian family. Our focus centered on mutations in the NR2E3 gene, which plays a critical role in the development and maintenance of the retina.MethodsTwenty‐five family members showed symptoms of RP, and fourteen of them underwent clinical examinations conducted by geneticists and ophthalmologists. The DNA samples of five individuals diagnosed with RP from the family were subjected to whole‐exome sequencing (WES) as part of the study. The candidate variant identified through WES was subsequently confirmed using bidirectional sequencing in additional family members. Additionally, in silico analysis, including molecular modeling, protein–protein docking, and molecular dynamics simulation (MD), was employed to assess potential pathogenic effects associated with the candidate variants.ResultsOphthalmic examination revealed night blindness, which is a common symptom among affected individuals. Genetic analysis identified a homozygous missense variant (c.934G>A/p.R311Q) in NR2E3 exon 6, which co‐segregates with other affected family members. Furthermore, molecular docking analysis indicated potential disruption in the binding affinity between NR2E3 and NR1D1 proteins. In‐depth, molecular dynamics analysis, considering parameters such as RMSD, RMSF, and hydrogen bonding, revealed notable differences between normal and mutant protein complexes.ConclusionExploring the molecular interaction between NR2E3 and NR1D1 provides new insights into the pathogenic mechanism of the p.R311Q mutation in RP.
Comparison of community‐based clinical education implementation among US dental and dental hygiene education programs
Authors: Dana Edith , Fitzgerald Mark , Kinney Janet , Riaz Mona , Cullen Jennifer 
Published: November 2024

AbstractPurpose/ObjectivesLimited access to oral care disproportionately affects underserved populations. Community‐based clinical education (CBCE) could address this health inequity. The purpose of this study was to compare the identified barriers and benefits of implementing CBCE in the curricula of US dental and dental hygiene education programs.MethodsIn the fall of 2023, a survey developed through focus groups was sent to 387 CBCE leaders in US dental and dental hygiene programs that were accredited by the Commission on Dental Accreditation (CODA).ResultsThere were 129 survey responses for a 33% response rate. There were statistically significant differences in institutional barriers: losing school production (p = 0.04), COVID‐19 pandemic (p = 0.02), CODA process for accreditation of major sites (p < 0.01), CODA standards (p = 0.01), number of dental chairs in school clinic (p = 0.03), and length of dental/dental hygiene program too short (p < 0.01); and human resource barriers: student transportation (p < 0.01), faculty buy‐in (p = 0.01), and student housing (p < 0.01). Benefits included: raising student awareness of social determinants of health (p = 0.04), increasing oral health workforce (p < 0.01), and engagement with harder clinical cases (p < 0.01).Conclusion(s)There were differences in the identified barriers and benefits to implementing CBCE in the curricula of US dental and dental hygiene education programs. The findings could inform the feasibility of implementing CBCE in both education programs, as well as address health inequities in access to oral care.
Sonographic localisation of lymph nodes suspicious of metastatic breast cancer to surgical axillary levels
Authors: Fenech Michelle , Burke Tracey , Arnett Grace , Tanner Alisha , Werder Natasha 
Published: November 2024

AbstractThe axillary lymph node (LN) burden of breast cancer patients guides multidisciplinary management and treatment regimes. Sonographic imaging is used to identify the presence, number and location of axillary LNs suspicious of malignancy and used to guide nodal fine needle aspirations and biopsies. Axillary LNs suspicious of harbouring breast cancer metastasis can be localised to three surgical axillary levels, numbered according to their location relative to the pectoralis minor muscle and lymph flow. To sonographically identify and localise suspicious axillary LNs, an understanding of the axillary anatomy, muscular sonographic landmarks, surgical axillary levels, and the sonographic technique to image and distinguish between benign and suspicious LNs is required.
EEG Emotion Recognition Based on GADF and AMB‐CNN Model
Authors: Zhao Qian , Zhao Dandan , Yin Wuliang 
Published: November 2024

ABSTRACTDeep learning has achieved better results in natural language processing, computer vision, and other fields. Nowadays, more deep learning algorithms have also been applied in brain‐based emotion recognition. In the studies on brain‐based emotion recognition, deep learning models typically use one‐dimensional time series as the input and cannot fully leverage the advantages of the models in image classification or recognition. To address this issue, based on the publicly available SEED and DEAP datasets, the Gramian angular difference field (GADF) method was proposed to construct two‐dimensional image representation datasets: SEED‐GADF and DEAP‐GADF datasets, in the paper. Additionally, a convolutional attention mechanism model (AMB‐CNN) was introduced and its classification performance was validated on SEED‐GADF and DEAP‐GADF datasets. AMB‐CNN achieved an average accuracy of 90.8%, a recall rate of 90%, and AUC of 96.86% on SEED‐GADF. On DEAP‐GADF, the average accuracy, recall rate, and AUC respectively reached 96.06%, 96.06%, and 98.58% in the valence dimension and 96.11%, 96.11%, and 98.73% in the arousal dimension. Finally, the comparison results with various algorithms and ablation experiments proved the superiority of the proposed model.
Plant Growth‐Promoting Bacteria Associated With Some Salt‐Tolerant Plants
Authors: Beitsayahi Fatemeh , Enayatizamir Naeimeh , Nejadsadeghi Leila , Nasernakhaei Fatemeh 
Published: November 2024

ABSTRACTGiven the benefits of bacteria associated with the rhizosphere and phytoplane of halophytes, this research focused on examining the plant growth‐promoting characteristics of bacteria isolated from Cressa cretica, Suaeda aegyptiaca, and Alhagi graecorum. From the 33 isolates tested, 9 exhibited plant growth‐promoting traits. Bacillus rugosus strain CS5 and Bacillus sp. strain SS4 exhibited the notable growth inhibition of the pathogenic fungus Fusarium oxysporum, with values of 47% and 45%, respectively. Bacillus sp. strains SS4 and CS1 demonstrated impressive results in solubilizing phosphorus and zinc, respectively, achieving concentrations of 259 and 271 mg L−1. Additionally, Staphylococcus xylosus strain SR2, Bacillus sp. strain SS4, and Bacillus paralicheniformis strain CR1 thrived in nitrogen‐free media. The Priestia filamentosa strain AL4 showed the greatest HCN production, whereas B. paralicheniformis strain CR1 was notable for higher auxin production. The Bacillus sp. strains SS4 and CS1 exhibited greater tolerance than other isolates in a medium containing 600 mM of NaCl. Additionally, inoculating these isolates into soil significantly alleviated the salinity and drought stress on Zea mays seedlings. These findings suggest that further investigation into these strains as microbial inoculants could be beneficial for mitigating salt and drought stress in plants.
What do we know about micronutrients in critically ill patients? A narrative review
Authors: de Man Angelique M.E. , Stoppe Christian , Koekkoek Kristine W.A.C. , Briassoulis George , Subasinghe Lilanthi S.D.P. , Cobilinschi Cristian , Deane Adam M. , Manzanares William , Grințescu Ioana , Mirea Liliana , Roshdy Ashraf , Cotoia Antonella , Bear Danielle E. , Boraso Sabrina , Fraipont Vincent , Christopher Kenneth B. , Casaer Michael P. , Gunst Jan , Pantet Olivier , Elhadi Muhammed , Bolondi Giuliano , Forceville Xavier , Angstwurm Matthias W.A. , Gurjar Mohan , Biondi Rodrigo , van Zanten Arthur R.H. , Berger Mette M. ,   ESICM/FREM MN group
Published: November 2024

AbstractMicronutrient (MN) status alterations (both depletion and deficiency) are associated with several complications and worse outcomes in critically ill patients. On the other side of the spectrum, improving MN status has been shown to be a potential co‐adjuvant therapy. This review aims to collect existing data to better guide research in the critical care setting. This narrative review was conducted by the European Society of Intensive Care Medicine Feeding, Rehabilitation, Endocrinology, and Metabolism MN group. The primary objective was to identify studies focusing on individual MNs in critically ill patients, selecting the MNs that appear to be most relevant and most frequently investigated in the last decade: A, B1, B2, B3, B6, folate, C, D, E, copper, iron, selenium, zinc, and carnitine. Given the limited number of interventional studies for most MNs, observational studies were included. For each selected MN, the review summarizes the main form and functions, special needs and risk factors, optimal treatment strategies, pharmacological dosing, and clinical implications all specific to critically ill patients. A rigorous rebalancing of research strategies and priorities is needed to improve clinical practice. An important finding is that high‐dose monotherapy of MNs is not recommended. Basal daily needs must be provided, with higher doses in diseases with known higher needs, and identified deficiencies treated. Finally, the review provides a list of ongoing trials on MNs in critically ill patients and identifies a priority list of future research topics.
The Effects of HIPEC on Survival of Gastric Cancer Patients With Peritoneal Metastasis
Authors: Ji Zhong‐He , Zhao Qi‐Di , Li Yan 
Published: November 2024

ABSTRACTCytoreductive surgery (CRS) plus hyperthermic intraperitoneal chemotherapy (HIPEC) has been recommended by Peritoneal Surface Oncology Group International (PSOGI) and several other academic organizations as the treatment of choice for selected patients with gastric cancer peritoneal metastasis (GCPM). This review updates the recent progress in CRS+HIPEC for GCPM, with particular focus on the potential curing effects of this approach for subclinical GCPM patients.
Genes involved in carbon, nitrogen, and sulfur cycling in an important estuarine ecosystem show coherent shifts in response to changes in environmental conditions
Authors: Preheim Sarah Pacocha , Morris Shaina , Zhang Yue , Holder Chris , Arora‐Williams Keith , Gensbigler Paul , Hinton Amanda , Jin Rui , Pradal Marie‐Aude , Buchanan Morgan , Gnanadesikan Anand 
Published: November 2024

AbstractWhile metagenomics can provide insight into microbial community metabolic potential, understanding factors that influence gene abundance is necessary to maximize the information gained from this analysis. Gene abundances are influenced by chemical or physical conditions along with other factors, such as copy number variation between taxa, methodological biases, or issues associated with identification and classification. Here, we identify major drivers of spatiotemporal shifts in microbial gene relative abundance from multiple months, sites, and depths within Chesapeake Bay in 2017 using shotgun metagenomics. We compared changes in relative abundance of key genes for bacterial photosynthesis, nitrogen, and sulfur metabolism with each other and measured environmental variables. Major drivers of differences in key metabolic gene abundances are associated with environmental variables that largely change with depth and season (e.g., temperature, oxygen, phosphate). For sulfur oxidation, bacterial photosynthesis, and denitrification, genes within each process are generally significantly correlated with each other and with several environmental variables. For other processes, such as nitrification, nitrogen fixation, and dissimilatory nitrate reduction to ammonium, genes that encode enzymes within the same pathway are not well correlated. The lack of correlation typically results from differences in identified taxa carrying these genes, suggesting modular pathway structure, methodological errors, or discrepancies in gene copy number between taxonomic groups. To be suitable indicators of biogeochemical processes for models, genes or pathways should be strongly correlated with environmental variables and specific to and inclusive of all taxa mediating the associated process.
Production of Polyvinyl Alcohol/Amoxicillin – Chitosan/Collagen Hybrid Bilayer Membranes for Regeneration of Gingival Tissues
Authors: Aydin Ayca , Ulag Songul , Nouri Sabereh , Durasi Elif , Pelit Arayıcı Pelin , Tinaz Gülgün Bosgelmez , Güncü Mehmet Mücahit , Cakir Rabia , Gunduz Oguzhan , Ustundag Cem Bulent 
Published: November 2024

AbstractPeriodontal diseases, if untreated, can cause gum recession and tooth root exposure, resulting in infection and irreversible damage. Traditional treatments using autologous grafts are painful and often result in postoperative complications. Scaffolds offer a less invasive alternative, promoting cell proliferation and healing without additional surgery, thus enhancing comfort for patients and doctors. This study developed Chitosan (Chit)/Collagen (Col) film surfaces and drug‐loaded Polyvinyl Alcohol (PVA)/Amoxicillin (AMX) nanofibers using solvent casting and electrospinning methods, respectively. The surfaces are characterized by scanning electron microscopy (SEM), mechanical testing, Fourier Transform Infrared Spectroscopy (FTIR), and differential scanning calorimetry (DSC). Biocompatibility and antimicrobial properties are assessed using NIH/3T3 fibroblast cells and bacterial cultures. SEM images confirmed the structural integrity of AMX‐loaded 13% PVA nanofibers, while FTIR analysis validated the compositional integrity of PVA/AMX nanofibers and Chit/Col film hybrid surfaces. Cell studies showed over 90% viability for Chit/Col film + PVA/AMX nanofiber hybrid bilayer membranes, confirming their biocompatibility. The antimicrobial assessment indicated that the Chit/Col film + PVA/AMX (0.2%) nanofiber hybrid bilayer membrane exhibited superior efficacy against Streptococcus mutans. These findings suggest that this hybrid bilayer membrane can enhance cell growth, promote proliferation, and enable controlled drug release, offering significant promise for regeneration of gingival tissues.
Development of Mg‐Alginate Based Self Disassociative Bio‐Ink for Magnetic Bio‐Patterning of 3D Tumor Models
Authors: Coban Basak , Baskurt Mehmet , Sahin Hasan , Arslan‐Yildiz Ahu 
Published: November 2024

AbstractAlginate forms a hydrogel via physical cross‐linking with divalent cations. In literature, Ca2+ is mostly utilized due to strong interactions but additional procedures are required to disassociate Ca‐alginate hydrogels. On the other hand, Mg‐alginate hydrogels disassociate spontaneously, which might benefit certain applications. This study introduces Mg‐alginate as the main component of a bio‐ink for the first time to obtain 3D tumor models by magnetic bio‐patterning technique. The bio‐ink contains magnetic nanoparticles (MNPs) for magnetic manipulation, Mg‐alginate hydrogel as a sacrificial material, and cells. The applicability of the methodology is tested for the formation of 3D tumor models using HeLa, SaOS‐2, and SH‐SY5Y cells. Long‐term cultures are examined by Live/dead and MTT analysis and revealed high cell viability. Subsequently, Collagen and F‐actin expressions are observed successfully in 3D tumor models. Finally, the anti‐cancer drug Doxorubicin (DOX) effect is investigated on 3D tumor models, and IC50 values is calculated to assess the drug response. As a result, significantly higher drug resistance is observed for bio‐patterned 3D tumor models up to tenfold compared to 2D control. Overall, Mg‐alginate hydrogel is successfully used to form bio‐patterned 3D tumor models, and the applicability of the model is shown effectively, especially as a drug screening platform.
Investigation of Release Kinetics of DOX from Polydopamine Nanocapsules Prepared by Hard Template Method
Authors: Abdollahi Elahe , Haddadi‐Asl Vahid , Ahmadi Hanie , Shirjandi Mastoure , Khanipour Fatemeh 
Published: November 2024

AbstractDevelopment of smart drug delivery systems (DDSs) for effective delivering drugs to targeted areas and achieving controlled drug release (CDR) is critical for cancer chemotherapy. The purpose of this study is synthesis of polydopamine (PDA) nanocapsules and analyze the adsorption and release properties of doxorubicin (DOX). PDA nanocapsules are manufactured using hard template approach. The influence of various parameters such as pH, adsorption time, and initial DOX content on the adsorption and release process is investigated. The resulting adsorption isotherm is consistent with the Langmuir isotherm, indicating that DOX adsorption on PDA nanocapsules is homogenous, uniform, and monolayer. PDA nanocapsules have an adsorption capacity of 689.6 mg g−1 under alkaline conditions, which is attributed to phenol group deprotonation mechanism and electrostatic repulsion. The adsorption kinetics are more consistent with the pseudo‐second‐order model. Furthermore, raising initial concentration of DOX results in a greatly increased adsorption capacity due to a larger driving force. Among the several parameters that can influence the pace and degree of DOX loading and release, local pH is regarded as a significant environmental component in the processes. Thus, pH‐responsive PDA nanocapsules have a significant potential for usage in locations with aberrant pH level, such as cancer tissue.
Proof‐of‐principle of 3D‐printed track‐end detectors for dosimetry in proton therapy
Authors: Bietenbeck Vicky , Bäcker Claus Maximilian , Wulff Jörg , Timmermann Beate , Bäumer Christian 
Published: November 2024

AbstractBackgroundDosimetric equipment in particle therapy (PT) is associated with high costs. There is a lack of versatile, tissue‐equivalent detectors suitable for in‐vivo dosimetry. Faraday‐cup (FC) type detectors are sensitive to stopped protons, that is, to track‐ends (TEs). They experience a renaissance in PT as they can cope with high dose rates. Owing to their simple functional principle, production of FC could benefit from the dynamic technological developments in additive manufacturing of sensors.PurposeTo build FC‐type detectors for PT by standard 3D‐printing. This study seeks to build an integrating, single‐channel (SC) FC for replacement of a traditional FC and a array of FC elements indicating the feasibility of a spatially resolving detector.MethodsSamples of FCs were produced with a dual‐extruder 3D‐printer with polylactic‐acid filaments, which contained graphite in the conductive parts of the detector. Production was optimized in terms of materials and printing temperature. Samples were characterized by electrical tests and non‐destructive 3D x‐ray imaging. Beam tests were conducted at a clinical PT machine.ResultsOperational FC‐type detectors for proton fields were printed. The detected charge of the SC FC corresponded qualitatively to the one of a traditional FC. A FC array was fabricated in a single run. There was a linear relationship between the response of the individual FC elements and the machine output.Conclusions3D‐printing is a viable method for producing low‐cost, tissue‐equivalent, FC‐type detectors for PT. They could potentially be used as TE detectors in anthropomorphic phantoms.