bluuebunny/crossref_metadata_2025_split huggingface dataset:
PROPERTIES OF NATIVE PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAE
Authors: Vorobyev D. S. , Semenova I. B. , Volokh Yu. V. , Romanenko E. E. , Baturo A. P. , Mikhailova N. A. 
Published: September 2019

Aim. The study of immunochemical and immunobiological properties of native protein-containing antigens of pneumococcus. Materials and methods. The study was carried out on the strains of the Collective Usage Center «Collection of Mechnikov Res. Inst. for Vaccine and Sera». In the work studied the chemical composition, the molecular weight of the obtained antigens in SDS-electrophoresis and antibody titers in ELISA. Protective activity of protein-containing antigens of pneumococcus was determined in experiments of active protection of mice. Results. Protein-containing antigens of pneumococcus were isolated from S. pneumoniae serotypes 3, 6B, 10A, 14, 19F, 23F and 36. The chemical composition of the preparations contained from 16 to 35% protein. In SDS-electrophoresis in polyacrylamide gel it was established that the molecular weight of protein-containing antigens of pneumococcus ranged from 14 to 116 kDa. Using ELISA shows the cross-activity of native antigens. Virtually all drugs reacted with antimicrobial rabbit serum obtained to serotype 19F (p≤0.05). Serotype serum 14 was less active and only protein-containing pneumococcal antigens obtained from 14 and 19F serotypes (p≤0.05) interacted with it. In the precipitation test according to Ouchterlony it was confirmed that preparations of serotypes 3, 6B, 14, 19F and 36 reacted with rabbit immune serum obtained for S. pneumoniae 19F serotype. In immunoblotting it was found that protein-containing antigens of pneumococcus isolated from serotypes 3, 6B, 10A, 14, 19F and 36 were associated with monoclonal antibodies to pneumococcal protein — pneumolysin. In vivo experiments it was shown that protein-containing antigens of pneumococcus protected animals from intraperitoneal infection of S. pneumoniae in homologous and heterologous systems (p≤0.05). Conclusion. The revealed immunochemical and cross-protective activity of protein-containing antigens of pneumococcus in vitro and in vivo experiments allows to select drugs derived from serotypes 6B, 10A, 19F and 36, as the most promising for further study of the intraspecific protective activity of individual native proteins of pneumococcus.
AUTOPHAGY REGULATION BY RUBELLA VIRUS
Authors: Gulimov M. K. , Romantsova L. R. , Astapenko A. V. , Schetinina Yu. R. , Prokofeva E. V. , Movsesyan G. V. , Zverev V. V. , Ammur Yu. I. 
Published: September 2019

Aim. Some viruses can subvert host defense mechanism, autophagy, to their own benefit. We analysed the effect of Rubella virus (RV) infection on autophagy in human alveolar epithelial cells A549. Materials and methods. Cells were infected with the wild type and lab-attenuated strain, C-77w and C-77a, respectively, with a multiplicity of infection of 1.0, in parallel, the expression level of genes encoding Beclin1, Atg5, Rab7, and p62 (SQSTM1) proteins participating in different steps of autolysosome formation was measured. To investigate the role of autophagy on RV replication cycle, we measured the amount of infectious RV particles, together with the viral RNA in supernatants and cell lysates, after incubation of A549 cells with wild type or attenuated strain in the presence of the autophagy inhibitor, Bafilomycin A1, or inducer, Rapamycin. Results. The significant increase in Beclin1 and Atg5 gene expression at 24-48 (for the wild type) and 24-72 (for the attenuated type) hours after infection was observed, while significant induction of either Rab7 or SQSTM1 gene expression was not noticed. This effect was correlated with more delayed increase of IFNβ expression and IFNβ-mediated pro-apoptotic gene expression leading to apoptotic cell death 72-96 hours after infection. Moreover, Bafilomycin A1 diminished the RV infection non significantly, as evidenced by the RT-qPCR and plaque assay, while Rapamycin increased the amount of infectious RV particles released by the infected cells more dramatically with wild type comparing with attenuated strain. Conclusion. Thus, we hypothesized that RV can use an antiviral mechanism to prevent degradation and ensure its replication, differentially regulating the process of autophagy, by stimulating the initiation and suppression of later steps.
POLYCOMPONENT VACCINE IMMUNOVAC-VP-4 AND IMMUNOTHERAPEUTIC CONCEPT OF ITS USE FOR THE PREVENTION AND TREATMENT OF DISEASES CAUSED BY OPPORTUNISTIC MICROORGANISMS
Authors: Egorova N. B. , Kurbatova E. A. , Akhmatova N. K. , Gruber I. M. 
Published: September 2019

Aim. Analysis of experimental data and results of long-term clinical studies of immunotherapeutic and prophylactic effectiveness of a polycomponent vaccine, Immunovac-VP-4. Materials and methods. Immunogenic strains of Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris, Escherichia coli, which antigens possessed broad intra — and interspecific cross-protective activity were selected to obtain the vaccine. Immunological parameters were determined using flow cytometry with the use of diagnostic monoclonal antibodies to cytokines, Toll-like receptors, surface molecules of dendritic cells and lymphocytes. The clinical effect of Immunovac-VP-4 was evaluated in chronic inflammatory and allergic diseases, as well as in the prevention of acute respiratory diseases (ARI) in children’s organized groups. Results. Immunovac-VP-4, protected the mice from infection caused by etiologically significant strains of bacteria and viruses, increased TLR-2, 4, 9-expressing cells; maturing the dendritic cells; the synthesis of cytokines IL1β, IL-6, IL-10, IL-12, IFNγ,; proliferation and increased cytotoxicity of NK cells. Significant clinical effect (69.2 — 100)% of Immunovac-VP-4 on the background of the basic therapy was obtained in patients with chronic inflammatory and allergic diseases. The therapeutic effect was accompanied by a positive dynamics of immunological parameters, in particular, an increase in IFNγ and a decrease in IL-4. Immunotherapy and immunoprophylaxis using Immunovac-VP-4 led to a decrease in the number of episodes of acute respiratory infections and their bacterial complications, including children in organized groups. Conclusion. Immunological feasibility of using Immunovac-VP-4 in complex treatment and prevention of bacterial and viral infections, caused by opportunistic microorganisms, as well as in allergic diseases was proved.
Comparative analysis of adaptation properties in typical and genetically altered Vibrio cholerae El Tor strains
Authors: Zadnova S. P. , Kritsky A. A. , Plekhanov N. A. , Cheldyshova N. B. , Smirnova N. I. 
Published: September 2019

Aim. Study of biological properties in natural strains of genovariants of V. cholerae biovar El Tor, affecting their adaptation capacities under nutrient deficiency while comparing them with typical strains. Materials and methods. Competitive sampling was carried out through plating a mixture of cells of the two strains under investigation into autoclaved river water. Growth rate was evaluated through the optic density values. Gene expression was studied applying RT-PCR with designed primers and probes. Results. It is established that during combined cultivation of V. cholerae El Tor typical strains and genovariants under the shortage of nutrient substances (autoclaved river water), at room temperature, the level of survivability in genetically altered strains is higher than in typical strains, which points to their expressed adaptation advantages over the typical ones under the stated conditions. It is demonstrated that selective benefits of genovariant strains are provided by higher cell growth rate and increased rpoS gene expression. Conclusion. Obtained have been new data on the ability of bacterial strains of V. cholerae El Tor genovariants to rapidly grow and better express global regulator of stress response, rpoS gene, which, probably, contributes to their enhanced adaptation not only under nutrient deficiency, but under the influence of other stress factors too.
Epidemiological and molecular-genetic monitoring of rotavirus infection in the Orenburg region in the period predictively
Authors: Akimkin V. G. , Gorelov A. V. , Podkolzin A. T. , Denisyuk N. B. 
Published: September 2019

Aim. To analyze the epidemiological situation of rotavirus infection in the Orenburg region, taking into account the manifestations of the epidemic process and the molecular genetic characteristics of rotavirus group. Materials and methods. We рresented the results of epidemiological and molecular genetic monitoring of rotavirus infection in the period 2013-2017. Molecular genetic typing was performed in 232 fecal samples of patients under 3 years of age with rotavirus infection. Were used methods of retrospective epidemiological analysis, ELISA, PCR. Results. The incidence of high tear and had a tendency to increase. The most affected were children under 14 years of age. The genetic potential of the rotavirus population in the region was represented by ten main genotypes. The leading significance in the epidemic process were genotypes with the highest frequency of occurrence: G4[P]8 (56,9%), G9[P]8 (12,9%), Mixt (8,6%), G2[P]4 (7,7%), G1[P]8 (6,5%). Conclusion. Were revealed a significant increase in the incidence of rotavirus infection among children. Were revealed the dominant genotypes of rotaviruses, their regional diversity and change during several seasons.
Epidemiology of HIV-assosiated tuberculosis in the Far Eastern Federal District
Authors: Bazykina E. A. , Trotsenko O. E. , Turkutyukov V. B. , Balakhontseva L. A. , Kotova V. O. 
Published: September 2019

Aim. To determine epidemiologic manifestations of HIV-infection associated with tuberculosis in the Far Eastern Federal District (FEFD). Materials and methods. The retrospective epidemiologic analysis was performed based on the official statistical data form № 61 «Data on HIV infected population» in reference to clinically registered HIV and tuberculosis co-infected patients. Results. The research revealed a growth trend of general tuberculosis prevalence against the background of stabilization of the tuberculosis incidence in HIV-positive patients in the FEFD during 2006-2015. High tuberculosis-burden was registered in the most of the Far Eastern federal district constituent entities (Khabarovsk and Primorsky regions, Jewish and Chukotka autonomous districts, Amursk oblast, and Republic Sakha (Yakutia). A statistically significant positive correlation (r=0,66, p=0,003) between percentage of active tuberculosis in HIV-positive patients and percentage of patients with AIDS was identified. The percentage of active tuberculosis in HIV-positive people on average over 10 years equaled to 65.0%. The prevalence rate of tuberculosis was higher by 19.2% in HIV-positive inmates compared with HIV-positive civilians. Conclusion. Тhe FEFD is a constituent entity of the Russian Federation with high and unstable levels of tuberculosis prevalence in people living with HIV.
The prevalence r ate of human herpes viruses among different age populations
Authors: Mardanly S. G. , Arseneva V. A. , Mardanly S. S. , Rotanov S. V. 
Published: September 2019

Aim. To evaluate infection with both individual types of HHV and their combinations for two categories of population — children and elderly persons. Materials and methods. The presence of IgG to human herpes viruses 1-6 and 8 types in sera of children aged 0-18 (1006 individuals) and elderly patients aged 60-99 (1004 individuals) was investigated by ELISA-method. Results. It was shown that 45 — 61,3% of examined children (aged 0-2 years) are infected with HHV-1, HHV-3, HHV-4, HHV-5 and HHV-6. The subsequent dynamics of infection with the first, third and fourth types of HHV expressed in the final constant growth up to 16-18 years was noted. There was no connection between children age and HHV-2, HHV-6 and HHV-8, it was ranged from 45 to 68% for HHV-6 and did not rise above 3.5 and 10% for HHV-2 and HHV-8 respectively. In most cases elderly persons of all age groups were infected with HHV-1, HHV-3, HHV-4 and HHV-5, antibodies to HHV-2 were detected in 21,3-26,6% of all individuals of this age. Conclusion. In order to ensure effective planning and implementation of adequate treatment and preventive measures that will minimize the adverse medical and social consequences of HHV spread among the population of the Russian Federation, it is necessary to continue detailed study of HHV infection in all population groups.
Possibility of using exometabolites Bifidobacterium bifidum № 791 in traumatology and orthopedics
Authors: Markov A. A. , Timokhina T. H. , Perunova N. B. , Paromova Ya. I. , Ivanova E. V. 
Published: September 2019

Aim. To study the effect of exometabolites of Bifidobacterium bifidum on the biological properties of causative agents of purulent-septic complications in trauma and orthopedic practice and to evaluate their effect on reparative bone tissue regeneration in the peri-implant zone in the postoperative period. Materials and methods. Using the museum cultures of B. bifidum 791, S. aureus 25923 ATCC, P. aeruginosa 27853 ATCC and antibiotic-resistant hospital strains, isolated from patients with periprosthetic infection and osteomyelitis (S.aureus 889, S. epidermidis 2041, P. aeruginosa 9672). The supernatant (exometabolites) of B. bifidum 791 was obtained from a broth culture, which was centrifuged and passed through a membrane filter. In studying the effect of exometabolites of bifidobacteria on the proliferative activity of bacteria, the two-day supernatant of B. bifidum was co-incubated for 24 cultures of museum and hospital strains. The biofilm formation in vitro experiment was studied after 24 and 96 hours. Treatment of implants with exometabolites of bifidobacteria was carried out for 30 minutes. Experimental research was carried out on rabbits of breed «Flandr». Titanium implants without coating (control) and with porous SBKFM coating treated with exometabolites of B. bifidum (experiment) were screwed into the tibial bones of animals. Histological examination and computed microtomography were performed using a SkyScan 1172 instrument (BRUKER). Result. Depending on the time of cultivation of bifidobacteria, the supernatant B. bifidum 791 had a bacteriostatic and bactericidal effect to varying degrees, and also inhibited the biofilm formation of the studied microorganism cultures, including antibiotic-resistant strains. In in vivo experiments, the use of porous SBKFM coatings on titanium implants treated with the supernatant of bifidobacteria made it possible to maintain the mineral density indices of the peri-implantation zone at a high level in comparison with the use of control titanium implants without coating. Conclusion. Positive results of preclinical experiments are promising for further clinical research in trauma and orthopedic practice, which ultimately will reduce the risk of developing septic complications and prevent the migration of metal structures and instability of endoprostheses in the postoperative period.
Pengaruh Latihan Small-Sided Games Terhadap Keterampilan Passing, Controlling dan Shooting Peserta Ekstrakurikuler Sepakbola SMK Negeri 1 Tegalsari Kabupaten Banyuwangi
Authors: Wardana Charaka Raga , Setiabudi Moh. Agung , Candra Arya T 
Published: September 2019

Passing, control, dan shooting merupakan teknik dasar yang paling dominan dilakukan dalam permainan sepakbola. Keterampilan ini harus dikuasai oleh pemain agar permainan menjadi lebih baik. Penelitian ini adalah penelitian eksperimen dengan memberikan perlakuan small side games. Subyek penelitian ini adalah peserta ekstrakurikuler sepakbola SMK Negeri 1 Tegalsari Kabupaten Banyuwangi. Hasil penelitian menunjukkan nilai sig 0,000 pada tes passing dan control. Sedangkan hasil tes shooting menunjukkan nilai sig sebesar 0,008 lebih kecil daripada derajat alpha 0,05. Dalam artian ada pengaruh yang signifikan pemberian perlakuann small side games terhadap keterampilan passing, control, dan shooting.
Abnormal QTc Syndrome in HIV-Infected Patients: A Systematic review of Prevalence and Risk Factors
Authors: Chastain Daniel B , Veve Michael P , Wagner Jamie L 
Published: September 2019

Background The purpose of this review is to critically analyse data regarding the prevalence and risk factors for developing a prolonged QTc interval and subsequent sudden cardiac death (SCD) in persons living with HIV (PLWH). Methods A systematic literature search using PubMed and Google Scholar databases was performed using the following search terms: ‘HIV and prolonged QTc’ and ‘managing HIV-patients with prolonged QTc’. References within articles of interest were also evaluated. Results/Discussion PLWH are at an increased risk of having a prolonged QTc interval. Some risk factors for this include the virus itself, concomitant medications, comorbid conditions, addictions and electrolyte disturbances. PLWH who have an increased HIV RNA viral load or decreased CD4+ T-cell count are at further risk for progressing to sudden cardiac death (SCD). Many medications commonly prescribed in the PLWH population, such as antiretrovirals and antimicrobials used in opportunistic infection prophylaxis, have also been shown to promote QTc prolongation through inhibition of human ether-a-go-go potassium channels or through drug metabolism inhibition of other QTc prolonging drugs. Conclusions Due to the high number of risk factors associated with QTc prolongation, clinicians should incorporate baseline and routine ECG monitoring for PLWH to potentially lower the increased risk of SCD in PLWH.
O potencial de mitigação da mudança climática dos vetores energéticos da cana-de-açúcar na frota paulistana de veículos leves
Authors: Camargo Augusto Tolentino , Simões André Felipe , Pacca Sérgio Almeida 
Published: September 2019

O presente trabalho quantificou o potencial energético da cana-de-açúcar enquanto matéria prima para a produção de etanol e bioeletricidade para a frota de veículos leves na cidade de São Paulo em relação ao uso da gasolina; concomitantemente, quantificou-se a correlata mitigação da emissão de gases do efeito estufa – GEE. Neste contexto, a partir de uma modelagem focada no incremento no uso de vetores energéticos derivados da cana-de-açúcar, foi calculada uma massa evitada de 116 Mt de CO2 até o ano de 2050. Consideramos um modelo no qual a partir de 2025, teria início uma evolução consistente do uso de veículos leves movidos a eletricidade. Para tanto, assumiu-se que, em 2050, o uso de combustíveis pela frota de veículos leves da cidade de São Paulo, seria igualitário, sendo um terço da frota movida à gasolina, uma terço movida à etanol e um terço movida à eletricidade. Os recursos energéticos derivados da cana-de-açúcar, como o etanol e a bioeletricidade (eletricidade gerada a partir da queima de resíduos da cana-de-açúcar) seriam suficientes para atender o cenário proposto. No modelo, observa-se que, com a queda do uso na gasolina, cai, também, a demanda por etanol, visto que a vigente legislação brasileira prevê que a gasolina comercializada tenha em sua composição 27% de etanol anidro. Uma vez reduzida a demanda por etanol para adição na gasolina, há a correlata redução da necessidade de produção de cana-de-açúcar e, assim, consequentemente, uma redução da área de plantio. Esta redução, em contrapartida, poderia, por exemplo, implicar em mais área para plantação de gêneros agrícolas alimentícios (uma externalidade tipicamente positiva).
Learning from Mistakes &ndash; a Case of Pediatric Patient with Recurrent Whitlow
Authors: Raykova Vessela V. 
Published: September 2019

Background: Herpetic whitlow is a herpes simplex virus infection of the fingers or thumb characterized by erythema accompanied by painful non-purulent vesicles.&nbsp;
Aim: To draw attention to the typical appearance of herpetic whitlow and to distinguish it from bacterial infections and other skin diseases because of their different management.
Materials and methods: The patient&rsquo;s history, dermatological status, and scrapings from the vesicles were taken. The swabs were cultured for isolation of bacteria and fungi. DNA extraction and PCR were performed for detection of HSV.&nbsp;
Results: Repeated identical infections of the finger were found in the patients&rsquo; history, commonly associated with respiratory infections. The cultured swabs for bacterial or fungal detection remained negative. The scraping from vesicle used for viral detection showed positive HSV result.
Conclusion: It is important to distinguish herpetic whitlow from infectious and skin diseases and to learn from yet done mistakes - the patient was previously diagnosed with bacterial whitlow, contact dermatitis and dermatitis of unknown origin.
Changes in the Contractile Activity and Reactivity to 5-HT of Smooth Muscles of Rats Following Total Body Irradiation with Accelerated Electrons
Authors: Charilaos Xenodochidis A. , Ardasheva Raina G. , Popov Veselin G. , Prissadova Natalia A. , Turiyski Valentin I. , Krustev Athanas D. , Vlaykova Mariya I. , Grudeva-Popova Zhanet G. , Sopadzhieva Marieta V. 
Published: September 2019

Background: Besides its &ldquo;classical&rdquo; neurotransmitter function in the central and peripheral nervous systems, serotonin, or 5-hydroxytryptamine (5-HT) is also a local hormone in a number of tissues, including those of the GI tract. Radiation is known to be able to disrupt certain functions of the tract, modulated by 5-HT-signaling pathways, or the serotonin receptors themselves.
Aim: The present investigation focused on clarifying the nature and extent of influence of an accelerated electron beam with energy of 9 MeV on the serotonergic mediation of healthy smooth muscle gastric tissue of rats following total body irradiation of the animals.
Materials and methods: The study involved a control group and two experimental groups of animals exposed to 1 and 5 Gy, respectively, using Siemens Primus S/N 3561. Circular smooth muscle tissues were isolated from rats 1 hour and 18 hours after they were exposed to 1 and 5 Gy and also 5 days after irradiation from the rats that received a dose of 5 Gy in order to investigate the action of exogenous serotonin at increasing concentrations from 10-8 to 10-4 mol/l. The contractile reactivity of each group SM preparations was registered isometrically.
Results: Electron beams with energy of 9 MeV did not damage the contractile apparatus of gastric SM of rats and had a stimulating effect on contractility resulting from rapidly developing processes (1 hour) or later occurring once (5 days).
Conclusions: Difference was observed in the importance of the factors of received dose, lapse of time from irradiation to investigation of SM tissues, and exogenous 5-HT concentration for the changes in SM reactivity in serotonin-induced tonic and phasic responses.