bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profile of Crude Protein Tyrosine Kinase on Plasma Membrane of Merino Sheep Spermatozoa Using the Method of SDS-Page (Sodium Dodecyl Sulphate-Polyacrilamide Gel Electrophoresis)
Authors: Wardani Vilda Carlenia , Madyawati Sri Pantja , Hastutiek Poedji 
Published: March 2018

The research aims to find out profile of crude protein tyrosine kinase on plasma membrane of Merino sheep spermatozoa. The research used fresh semen sample collected using artificial vagina technique, next it was centrifuged to separate spermatozoa from seminal plasma. Then, spermatozoa (pellet) was isolated. After obtaining spermatozoa isolate, analysis on protein tyrosine kinase using SDS-PAGE method was conducted. The method to analyze protein using SDS-PAGE to separate protein based on molecular weight. Analysis on molecular weight was conducted by comparing result of isolate tapes with protein marker tapes. Running on SDS-PAGE gel isolate protein plasma membrane of Merino sheep spermatozoa in the research resulted in 13 protein tapes with average molecular weights of 115.44 kDa; 95.78 kDa; 78.22 kDa; 69.02 kDa; 62.33 kDa; 53.72 kDa; 40.56 kDa; 30.74 kDa; 21.16 kDa; 13.67 kDa; 10.85 kDa; 9.49 and 8.07 kDa kDa. From this result, the second tape with the molecular weight of 95.78 kDa was believed to be protein tyrosine kinase tape however it needs to be confirmed further using Western Blot method.Keywords: tyrosine kinase, membrane of Merino sheep spermatozoa , SDS-PAGE
Effect of L-Arginine on the Thickness Iliac Arteries Wall Post Fogarty Balloon Embolectomy Catheter in Rabbit (Oryctolagus cuniculus)
Authors: Puspitarani Gavrila Amadea , Widjaja Ngakan Made Rai , Primarizky Hardany 
Published: March 2018

The effect of the chronic administration of L-arginine on iliac arteries thickness after surgical embolectomy using Fogarty balloon catheter were examined. Sixteen New Zealand White male rabbits underwent unilateral iliac artery surgical embolectomy using Fogarty balloon catheter. Then the animals were divided into two group of control (P0) fed with standard rabbit diet and treatment (P1) standard rabbit diet added with L-arginine in their drinking water 4 for weeks. The iliac arteries were harvested for histological slide preparation with routine method, observed under the microscope attached with Nikon camera and measured using NIS-Element BR application in 8 zones. Data were collected and analyzed by independent T-test. The result of the vessel thickness was significantly decreased on the P1 (234.1± 18.1 µm) than P0 (411.6 ± 191.7 µm). Furthermore, the thickness of intimal was significantly decrease in P1 (140.9± 27.0 µm) compared with P0 (304.3± 215.7 µm). As a result, there was no significant different on the thickness of medial between P1 (93.2± 15.7 µm) and P0 (107.3± 101.5 µm). The results demonstrate that L-arginine decreased iliac artery wall thickness after surgical embolectomy with Fogarty balloon catheter in rabbit. Keywords: L-arginine ; nitric oxide ; Fogarty balloon catheter ; vascular smooth muscle cell proliferation.
Ellagic Acid Activity in Healing Process of Incision Wound on Male Albino Rats (Rattus norvegicus)
Authors: Primarizky Hardany , Yuniarti Wiwik Misaco , Lukiswanto Bambang Sektiari 
Published: March 2018

This study was conducted to prove whether ellagic acid can be used as a regulator of incision wound healing process in male albino rats as experimental animals. A total of 24 male healthy rats based on physical examination, aged at 3 months old and weight at 150-180 grams used in this study. Rats were divided into three groups and each group has 8 replications, group of control (P0) has no incision wound and no medication, treatment group (P1) has incision wound and treated with Betadine® and (P2) has incision wound and treated with 2,5 % ellagic acid. The variable that observed are histopathology changes (collagen, PMN, angiogenesis and fibrosis) from the male albino rats. The samples collection was taken at day 15 and data were collected and analyzed by Analysis of Variance (ANOVA) test. The result of collagen and PMN cells score in the control group (P0) were 1.80 ± 0.16 and 1.6 ± 0.08 with mild angiogenesis and no fibrosis. The results in the treatment (P2) group, where the collagen and PMN scores were 1.60 ± 0.08 and 1.5 ± 0.8, accompanied by mild angiogenesis and fibrosis. Meanwhile, in the treatment (P1) group, the results of scoring of collagen and PMN were lower than the other groups, 0, 80 ± 0.08 and 0.6 ± 0.16 accompanied by moderate angiogenesis and fibrosis. There was a significant difference of collagen scoring, PMN cells number, fibrosis and angiogenesis among the treatment groups (p<0.05). P1 treatment group has significant different with treatment groups P0 and P2 (p<0.05), but there is no significant difference between treatment groups P0 and P2 (p>0.05). From the results, it can be concluded that the topical application of ellagic acid ointment was proved to provide healing process on incision wound. Topical administration of ellagic acid ointment for 14 days was able to improve the skin condition and promoted the wound healing.Keywords: Ellagic acid ; wound healing ; collagen ; angiogenesis ; fibrosis ; anti-inflammatory ; antioxidant ; antibacterial.
Control and Preventive Study of Brucellosis by Using
Authors: J Rahmahani , D Handijatno , W Tyaningsih , Suwarno Suwarno 
Published: March 2018

The aims of this research is to determine the ability of sub unit lipopolysacharide(LPS) vaccine of Brucella abortus strain S-19 in mice and goat, including IgM and sub classes IgG antibody humoral response, cellular mediated immune response (IL-2, IFN- γ) in mice, also IgG as humoral immunity, IL-4 and IL-12 as cellular immunity, comparison affectivity with Brucella abortus strain RB-51 vaccine in goat . This research has two steps methods. Step first, 30 Balb C mice were divided into 3 groups and vaccinated subcutaneously, First group injectedB. abortus S-19, second group injected LPS and third group injected sodium chloride solution. Booster vaccination was conducted every two weeks till the eight week after first vaccination. The second step performed vaccinated to 30 goats divided into three groups. First group was injected by subcutaneous LPS 50 µg/ml and second group injected LPS 100 µg/ml and the third group injected with sodium chloride as control. Booster vaccination conducted 2 weeks after first vaccination and second vaccination. Result of the research conferred. Result research, antibody response in mice showed vaccination by LPS of B. abortus S-19 showed higher titer than vaccination by whole cells but inverse cellular response. The both vaccines showed induce subclass antibody response, vaccination by LPS tendency to IgM response but vaccination by Whole cells active vaccine tendency to IgG1, IgG 2a and IgG2b. Response antibody in goat on two weeks after first vaccination, vaccination with LPS of B. abortus S-19, dose 50 µg/ml failed or zero titer IgG response but dose 100 µg/ml was 500response antibody on two weeks after second vaccination by dose 50 µg/ml was 340 but by dose 100 µg/ml was 960, while cellular IL-12 response two weeks after first vaccination by dose 50 µg/ml was 22.88 pg/ml but by 100 µg/ml was 62.15 pg/ml. Response cellular IL -12 two weeks after second vaccination 50 µg/ml was 12.04 pg/ml while by dose100 µg/ml was 130.88pg/ml Cellular immune response IL-4 on two weeks after first vaccination, dose 50 µg/ml showed 55.57 pg/ml but by dose100 µg/ml was 49.35 pg/ ml. Response cellular IL-4 on two weeks after second vaccination by dose 50 µg/ml was 22.17 pg/ml but by dose 100 µg/ml was 143.89 pg/ml Keyword: Vaccine sub-unit LPS of Brucella abortus S-19, Humoral antibody, Cellular antibody
Hispathology of Coronary artery of male rat (Ratus Norvegicus) with high fat diet after being given ethanol extract of Indian acalypha (Acalipha indica. L)
Authors: R Kurnijasanti , D Winarti , R.S. Wahyuni , K Puguh , Setyabudi Setyabudi , Sukmanadi Sukmanadi , R Sugihartuti , R Damayanti , N Hidayati , K Rahmawati , A.S. Sudjarwo 
Published: March 2018

This research aims to know the effect of ethanol extract of Indian acalypha (Acalipha indica L) to histopathology of coronary artery of male rat (Ratus norvegicus) with high fat diet. Twenty male rats (Rattus Norvegicus) males aged 3-5 months with mean weight 200 grams were divided into 5 groups and 4 replications. Group 1 (negative control) was only given standard feed and distilled on ad libidum. Four groups (Positive control, Treatment 1, 2 and 3) were made hypercholesterolaemia by administering 1 mL peroral pork oil for 56 days. On day 14, the cholesterol levels were checked. After hypercholesterolaemia, from day 15 to day 56, each group was given ethanol extract of Indian acalypha peroral with a dose of 200 g / kg BW (P1), 400 g / kg BW (P2) and 800 g / kgBW (P3), whereas the positive control groups were not given ethanol extract of Indian acalypha. On day 57, the dying rat was dissected for the removal of the cardiac organ and the making of coronary artery histopathological preparation. Data on histopathologic observation of coronary artery were analyzed by the Kruskal Wallis test, and if there was a significant difference between treatment groups (p <0.05), then it continued with the Mann-Whitney test. The results showed that in infiltration of fat cells and endothelial cells that experienced damage treatment P1, P2, and P3 which were given extracts of Indian acalypha experienced decrease compared to positive control treatment groups. The result of statistical test of fat cells and endothelial cells in the coronary artery that experienced damage showed significant differences between treatment groups (p <0.05), whereas in the statistical test of coronary artery foam cells showed no significant differences between treatment groups (P> 0 , 05). From the research, it can be concluded that ethanol extract of Indian acalypha (Acalipha indica L) can improve histopathology coronary artery of rat fed high fat diet.
Immunohistochemical Detection of Porcine Reproductive and Respiratory Syndrome Virus Antigen in Formalin-Fixed, Paraffin-Embedded Tissues with Correlation to Clinicopathologic Data
Authors: Manzano Lavina Gracia G. 
Published: March 2018

Immunohistochemistry (IHC) was used in this study to detect the presence of porcine reproductive and respiratory virus (PRRSV) antigen using monoclonal antibody (Mab SDOW17) in formalin-fixed, paraffin embedded tissues of lung, lymph node, heart, spleen, and kidney of pre- weaning to less than 90 day old pigs. Out of the 25 tissue samples examined from swine cases of the Philippine Animal Health Center (PAHC), Bureau of Animal Industry and College of Veterinary Medicine (CVM) Histopathology Laboratory, University of the Philippines-Los Baños (UPLB), 14 or 56% (14/25) were IHC positive for PRRSV antigen. Among the selected tissue samples, the PRRS virus was detected the most in the lymph nodes (64%) and lungs (40%), respectively, compared to other organs such as spleen (33%), kidney (28%), and the heart (17%). Only 43% (6/14) of cases coincided with the pathogenesis and clinicopathologic lesions of PRRS which are proliferative interstitial pneumonia and lymphoid follicular hyperplasia and necrosis. PRRS positive cases in IHC were consistently found to have co-infections with viral and bacterial diseases. Since PRRSV has tropism for macrophages and destruction of these cells leads to immunosuppression, affected animals are vulnerable to secondary infections. Keywords: formalin-fixed; immunohistochemistry; paraffin embedded tissue; piglets; Porcine Reproductive and Respiratory Syndrome; PRRS virus antigen; Mab SDOW17
Anthelmintic Activity of Ocimum sanctum Linn. Leaves Ethanol Extract Against Fasciola gigantica in vitro
Authors: Mahardika Mesia Margi , Sudjarwo Sri Agus , Koesdarto Setiawan 
Published: March 2018

The aims of this research are to know the concentration, exposure time and interaction between concentration and exposure time of Ocimum sanctum Linn. leaves ethanol extract which cause the most mortality toward Fasciola gigantica. Also to know its value of LC50 and LC90. The research was completely randomized design. There were five treatments. Each treatment was done in four replications and used 10 Fasciola gigantica. The observation and recording of dead Fasciola gigantica were done at 0, 2, 4, 6, 8 and 10 hours. Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (50ºC). The obtained data was analyzed using ANOVA Factorial and continued with Duncan Multiple Range Test. The result was 10% concentration and exposure time for 10 hours caused the most mortality toward Fasciola gigantica. However, the interaction between concentration and exposure time resulted that 5% concentration for 8 hours already caused the most mortality of Fasciola gigantica. Probit analysis was used to calculate the LC50 and LC90. The results were LC50 of Ocimum sanctum Linn. leaves ethanol extract was 7.9% at 4 hours, 3.7% at 6 hours, 1.8% at 8 hours and 0.8% at 10 hours and the LC90 was 8.4% at 10 hours. Key words: Ocimum sanctum Linn. leaves, Fasciola gigantica, ethanol extract, in vitro.
Scabiosis in Rabbit
Authors: Sofyan Miyayu Soneta , Chrismanto Doni 
Published: March 2018

Scabiosis caused by Sarcoptes scabiei and still hard to controled. So many factors that influence include geographic factors that play a role in the incidence of parasitic diseases as tropical Indonesiastrongly support the life cycle S.scabiei var.cuniculi. Control of scabies has been by way of drug delivery such as injection ivermectine scabies, permethrine cream, or the use of malathion liquid, the control method requires high costs due to scabies infections can occur repeatedly if the surrounding environment is not considered. Scabies or mange is a contagious skin disease due to mites (mites) Sarcoptes scabiei. All rabit in Animal Hospital will be identified symptoms of scabies like their crust a whitish, thickening of the skin, the hair shaven in the nose, muzzle, around the eyes, ears or if the infection is severe can be found in the legs or back. Further assessment (scoring) between positive score 1-4 based on the severity of the infection. Keywords: Scabies, Sarcoptes scabei, Rabbit, Ivermectin
Detection of Newcastle Disease Virus by Immunohistochemistry on the Brains of Laying Birds with Clinical Signs Torticollis and Curled Toe Paralysis
Authors: Wulan Ocie Harum , Yunita Niken , Wuryastuty Hastari , Wasito Raden 
Published: March 2018

Poultry is one of animal protein sources that have been consumed much in Indonesia. Various of health disorders are caused by environmental changes, woof and infectious agents have caused decline in production and economic loss. One of diseases that have a big impact on poultry husbandry is Newcastle disease (ND). Newcastle disease virus (NDV) is an important disease in Indonesia, because this has spread around Indonesia and caused a big loss for poultry industry moreover, this has high morbidity and mortality and very rapid spread of the virus. The purpose of the research is to find out and determine a fast, exact and accurate, and efficient and effective application immunohistochemistry streptavidin biotin (IHK SB) test on NDV diagnosis affirmation and for NDV fast detection as a cause of disease on commercial laying hens with clinical torticollis and curled toe paralysis symptoms. The samples used in the form of tissues from 20 commercial laying hens with an indication of clinical torticollis and curled toe paralysis symptoms. Brains would be tested using streptavidin biotin. Inspection result data obtained by immunohistochemistry streptavidin biotin (IHK SB) method is analyzed in a descriptive and qualitative way. The result of the research proves that IHK SB coloration, NDV antigen could be detected on brains. Based on the result of the research, it could be concluded that IHK SB could be applied for NDV diagnosis affirmation and it proves that commercial laying hens with clinical torticollis and curled toe paralysis symptoms. Keywords: Newcastle disease virus, immunohistochemistry streptavidin biotin, torticollis, curled toe paralysis
Blood Parasite Infection Prevalence in Kampong Chicken Breeder’s Group in Garut
Authors: Legowo Djoko , Khotimah Syifa Husnul , Suwanti Lucia Tri 
Published: March 2018

The Aim of this research was to determine the blood parasite infection prevalence in Garut District. The research was conducted in November-December 2016. 160 samples of blood were obtained from eight subdistrict at Kampong Chicken Breeder’s Group (KEPAK) in Garut District smeared with 3% Giemsa solution and examined under a microscope with 1000x magnification. The merozoite and gametocyte that was discovered recorded. The data that obtained was analyzed descriptively. A parasites were found in 11 blood samples (6.88%). The genus of blood parasites is Leucocytozoon sp., while Plasmodium sp., Haemoproteus sp., and Trypanosoma avium sp. are not found in this research (0%). All of blood infected parasite only found at Tarogong Kidul subdistrict and seven other subdistricts showed negative results. Keyword: Blood parasite, Leucocytozoon, KEPAK, Kampong Chicken
Amino Acid Analysis of Fusion (F) Gene and Prediction of Epitope B-Cell Newcastle Disease Surabaya Isolate As Vaccine Candidate
Authors: Rahmawati Indah Laili , Rantam Fedik Abdul , Tyasningsih Wiwik 
Published: March 2018

The aims of this research is to determine pathotype according to composition amino acid cleavage site of F protein, and epitope prediction of F protein from Surabaya isolate. Samples were cloacal swabs of 28 backyard chickens from five wet markets in Surabaya, 9 ND positive isolates from Laboratory of Virology and Immunology Department Microbiology Airlangga University, and also positive LaSota isolates (ATCC). Samples were isolated on the embryonated chicken egg and identified by HA test. The viruses were confirmed as NDV by hemagglutination inhibition assay and RT-PCR with target 687 bp. Prediction of epitope were using online software IEDB. The result of epitope B cell prediction from each isolate showing 11-12 epitope B cell candidates in protein F. Thus, it could be concluded that ND virus which isolated from Surabaya has a chance as immunogen candidate, which can be developed as vaccine candidate. Key words: Newcastle disease, Cleavage site, Fusion protein, Surabaya
Mini Review : Liver Fibrosis Mechanism
Authors: Yuniarti Wiwik Misaco , Primarizky Hardany 
Published: March 2018

Liver fibrosis is the process of wound healing in the liver that caused by a various causes. The longer time of wound healing process, the more severe disorder will be. This chronic condition is characterized by excessive deposition of extracellular matrix then ended with fribrosis. This mini review describes about the phase of liver fibrogenesis. Even though there are many variations of fibrogenesis in animals and human, in general there are three fibrogenic cells that involved in fibrogenesis. These three cells are fibroblast or endogenous fibroblast like-cells, epithelial to mesenchymal transition (EMT), and fibrocytes that derived from bone-marrow. Keywords : liver, fibrosis, fibroblast, EMT, fibrocytes, collagen
Immunogenicity of Bone Graft Using Xenograft Freeze-Dried Cortical Bovine, Allograft Freeze-Dried Cortical New Zealand White Rabbit, Xenograft Hydroxyapatite Bovine, And Xenograft Demineralized Bone Matrix Bovine In Bone Defect Of Femoral Diaphysis White Rabbit Experimental Study In Vivo
Authors: Ferdiansyah Ferdiansyah , Utomo Dwikora Novembri , Suroto Heri 
Published: March 2018

Bone defect remains a big challenge for orthopedic surgeon. Bone grafting nowadays become the second common transplantation after blood transfusion. Autogenous bone graft is the gold standard in treatment of bone defect, but it’s source limitation and donor site morbidity makes some surgeon were looking for allograft or xenograft. There are some issues with allo- and xenograft about difficulty in corporation and rejection reaction. This study explores the immunogenicity of allograft and xenograft. Keyword : freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, demineralized bone matrix xenograft.
Genotyping Analysis of Mycobacterium leprae isolated in Water Environment of Leprosy Endemic Places in Lamongan, East Java
Authors: Prakoeswa Cita Rosita Sigit , Herwanto Nanny , Wahyuni Ratna , Iswahyudi Iswahyudi , Adriaty Dinar , Agusni Indropo , Izumi Shinzo 
Published: March 2018

Finding of Mycobacterium leprae from water of leprosy endemic areas were reported. East Java Province is ranked number eight as the endemic area of leprosy in Indonesia and Lamongan district is the local area with the highest new cases reported. To study the transmission of Mycobacterium leprae infection in endemic areas, it is important to detect the distribution of Mycobacterium leprae in the environment and population, also to analyze the genetic variation pattern. Keywords: endemic; environment; genotyping; leprosy; Mycobacterium leprae.
Iron Overload Reduces Cholestrol and Triglyceride Serum of Mice
Authors: Setiawati Devi Agustin , Syamsunarno Mas Rizky A.A. , Fianza Pandji Irani , Atik Nur , Anggraeini Neni , Ghozali Mohammad , Safitri Ratu , Panigoro Ramdan 
Published: March 2018

Most of the case of patient with hemoglobin disorder genes need routine blood transfusion throughout their life. It leads to increase iron accumulation with liver as the main organ for iron storage. Liver is the main organ for metabolic process, including triglyceride and cholesterol. However, correlation between metabolism of triglyceride and cholesterol, and iron overload condition is remain uncertain. The purpose of this study was to investigate the effect of iron overload to triglyceride and cholesterol level in the serum of mice. Keywords: iron overload; Triglyceride serum; Cholesterol Serum.
Measurement of Alkaloids Achyranthes Aspera Linn Level Using Thin Layer Chromatography Method and High-Performance Liquid Chromatography
Authors: Meles Dewa Ketut , Wurlina Wurlina , Anom Adnyana Dewa Putu 
Published: March 2018

One of the plants with the potential to be developed into a raw material for anti-breast cancer drugs is jarong (Achyranthes aspera linn). Allegedly alkaloid of Achryranthes aspera linn leaves showed anticancer activity in vitro in mice myeloma cells is extremely potent. In vivo study showed that the alkaloid Achyranthes aspera linn causes apoptosis and healing in breast cancer cells in mice induced by benzopyrene. Alkaloids of Achyranthes aspera linn leaves as anticancer been listed on Incestual Property Rights (IPR) since October 12, 2012, with number P00201299839. This study aims to prove the existence of alkaloids in the leaves of Achyranthes aspera linn using thin layer chromatography (TLC), determine the level of alkaloids in the leaves alkaloid fraction of Achyranthes aspera using High-Performance Liquid Chromatography (HPLC) and alkaloid fraction dose as an anticancer and dosing for the study. Alkaloid qualitative measurement results with thin layer chromatography showed alkaloid in orange. Alkaloid levels using HPLC has a retention time of 3.482 minutes, a wavelength of 254 nm, there are 13 kinds of chemical substances with the major components amounted to 52.36% alkaloids, the other component is the green substance with a peak area of 13.0624%, while other chemical substances in a very small peak area of between 0.428% until 8.3598%. Alkaloid fraction dose of Achyranthes aspera used in the study as anticancer is controlled the dose of 0 mg/kg, 1 treatment dose of 60 mg/kg, 2 treatment dose of 75 mg/kg and 3 treatment dose of 90 mg/kg. Keywords: Alkaloids, Achyranthes aspera linn, TLC, HPLC.