bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long range channelrhodopsin-assisted circuit mapping of inferior colliculus neurons with blue and red-shifted channelrhodopsins
Authors: Goyer David , Roberts Michael T. 
Published: September 2019

ABSTRACTWhen investigating neural circuits, a standard limitation of the in vitro patch clamp approach is that axons from multiple sources are often intermixed, making it difficult to isolate inputs from individual sources with electrical stimulation. However, by using channelrhodopsin assisted circuit mapping (CRACM) this limitation can now be overcome. Here, we report a method to use CRACM to map ascending inputs from lower auditory brainstem nuclei and commissural inputs to an identified class of neurons in the inferior colliculus (IC), the midbrain nucleus of the auditory system. In the IC, local, commissural, ascending, and descending axons are heavily intertwined and therefore indistinguishable with electrical stimulation. By injecting a viral construct to drive expression of a channelrhodopsin in a presynaptic nucleus, followed by patch clamp recording to characterize the presence and physiology of channelrhodopsin-expressing synaptic inputs, projections from a specific source to a specific population of IC neurons can be mapped with cell type-specific accuracy. We show that this approach works with both Chronos, a blue light-activated channelrhodopsin, and ChrimsonR, a red-shifted channelrhodopsin. In contrast to previous reports from the forebrain, we find that ChrimsonR is robustly trafficked down the axons of dorsal cochlear nucleus principal neurons, indicating that ChrimsonR may be a useful tool for CRACM experiments in the brainstem. The protocol presented here includes detailed descriptions of the intracranial virus injection surgery, including stereotaxic coordinates for targeting injections to the dorsal cochlear nucleus and IC of mice, and how to combine whole cell patch clamp recording with channelrhodopsin activation to investigate long-range projections to IC neurons. Although this protocol is tailored to characterizing auditory inputs to the IC, it can be easily adapted to investigate other long-range projections in the auditory brainstem and beyond.SUMMARYChannelrhodopsin-assisted circuit mapping (CRACM) is a precision technique for functional mapping of long-range neuronal projections between anatomically and/or genetically identified groups of neurons. Here, we describe how to utilize CRACM to map auditory brainstem connections, including the use of a red-shifted opsin, ChrimsonR.
Context-dependent decision making in a premotor circuit
Authors: Wu Zheng , Litwin-Kumar Ashok , Shamash Philip , Taylor Alexei , Axel Richard , Shadlen Michael N. 
Published: September 2019

SummaryCognitive capacities afford contingent associations between sensory information and behavioral responses. We studied this problem using an olfactory delayed match to sample task whereby a sample odor specifies the association between a subsequent test odor and rewarding action. Multi-neuron recordings revealed representations of the sample and test odors in olfactory sensory and association cortex, which were sufficient to identify the test odor as match/non-match. Yet, inactivation of a downstream premotor area (ALM), but not orbitofrontal cortex, confined to the epoch preceding the test odor, led to gross impairment. Olfactory decisions that were not context dependent were unimpaired. Therefore, ALM may not receive the outcome of a match/non-match decision from upstream areas but contextual information—the identity of the sample—to establish the mapping between test odor and action. A novel population of pyramidal neurons in ALM layer 2 may mediate this process.
Agrobacterium-mediated floral-dip transformation of the obligate outcrosser Capsella grandiflora
Authors: Dew-Budd Kelly , David Brandon , Beilstein Mark A. 
Published: September 2019

AbstractPlant transformation by floral dip has been essential for research on plant genetics. The plant family Brassicaceae is one of the most well studied plant families and contains both established and emerging genetic model species. Two emerging model species that bear on the evolution of the selfing syndrome are Capsella grandiflora, an obligate outcrosser, and C. rubella, an inbreeder. While the selfing syndrome has been well characterized at the genomic level the genetic mechanisms underlying it remain elusive, in part due to the challenges of establishing mutation lines in C. grandiflora. Here, we describe an efficient method for transforming C. grandiflora by Agrobacterium-mediated floral-dip while simultaneously tracking self-incompatibility loci. With the ability to transform both C. grandiflora and C. rubella, researchers have gained a valuable tool to study the progression to selfing at the genetic level.
Octanol-water partition coefficient measurements for the SAMPL6 Blind Prediction Challenge
Authors: Işık Mehtap , Levorse Dorothy , Mobley David L. , Rhodes Timothy , Chodera John D. 
Published: September 2019

AbstractPartition coefficients describe the equilibrium partitioning of a single, defined charge state of a solute between two liquid phases in contact, typically a neutral solute. Octanol-water partition coefficients (Kow), or their logarithms (logP), are frequently used as a measure of lipophilicity in drug discovery. The partition coefficient is a physicochemical property that captures the thermodynamics of relative solvation between aqueous and nonpolar phases, and therefore provides an excellent test for physics-based computational models that predict properties of pharmaceutical relevance such as protein-ligand binding affinities or hydration/solvation free energies. The SAMPL6 Part II Octanol-Water Partition Coefficient Prediction Challenge used a subset of kinase inhibitor fragment-like compounds from the SAMPL6 pKaPrediction Challenge in a blind experimental benchmark. Following experimental data collection, the partition coefficient dataset was kept blinded until all predictions were collected from participating computational chemistry groups. A total of 91 submissions were received from 27 participating research groups. This paper presents the octanol-water logPdataset for this SAMPL6 Part II Partition Coefficient Challenge, which consisted of 11 compounds (six 4-aminoquinazolines, two benzimidazole, one pyrazolo[3,4-d]pyrimidine, one pyridine, one 2-oxoquinoline substructure containing compounds) with logPvalues in the range of 1.95–4.09. We describe the potentiometric logPmeasurement protocol used to collect this dataset using a Sirius T3, discuss the limitations of this experimental approach, and share suggestions for future logPdata collection efforts for the evaluation of computational methods.
Millennials’ intention to stay and word-of-mouth referrals
Authors: Dechawatanapaisal Decha 
Published: September 2019

PurposeThe purpose of this paper is to investigate the mediating effect of organizational identification in the relationship between person-organization fit and intention to stay as well as word-of-mouth referrals. The study also examines the role of perceived external prestige as a moderator of the relationship between organizational identification and intention to stay and word-of-mouth referrals.Design/methodology/approachData were collected from 2,649 millennial employees working in various companies located within the Central Thailand Industrial Estates. The hypothesized relationships were tested and analyzed by means of a confirmatory factor analysis, structural equation modeling, hierarchical regression and a bootstrapping procedure.FindingsThe results revealed that the direct relationships between person-organization fit and intention to stay as well as word-of-mouth referrals were found to be partially mediated by organizational identification. In addition, perceived external prestige was found to have a moderating effect on the relationship between organizational identification and word-of-mouth referrals, but found no effect on employee retention.Research limitations/implicationsThe current research took place among the millennial workers in Thai organizations, which needs to be extended to other generational cohorts or different culture settings for more generalization.Practical implicationsThe results imply that managers should routinely assess and monitor person-organization compatibility, and ensure that corporate cultures, values and norms are properly communicated and mutually shared among the millennial workers. The aim is to inspire them to perceive better fits and proudly identify with their workplace. Such efforts are likely to induce not only retention, but also should encourage word-of-mouth referrals.Originality/valueThis study extends existing knowledge by assessing the relationships among person-organization fit, organizational identification and perceived external prestige as well as their impacts on intention to stay and word-of-mouth referrals by millennial employees, which has not been extensively investigated in the literature.
Trends and changes in the International Journal of Entrepreneurial Behaviour & Research
Authors: Duran-Sanchez Amador , Alvarez García José , Del Río-Rama María de la Cruz , Ratten Vanessa 
Published: September 2019

Purpose
The International Journal of Entrepreneurial Behaviour & Research is amongst the most prestigious entrepreneurship journals and rapidly gaining ascendancy as a premier journal. It is a relatively young journal being 24 years old and in 2018 achieved inclusion on the Social Science Citation Index. The purpose of this paper is to analyse the development of the journal from birth to its current trends in order to project future potential research trends. This will enable an understanding of potential future research topics that will be influential in the entrepreneurship research field.

Design/methodology/approach
A bibliometric analysis is conducted using the Scopus database to analyse the most cited articles, authors, institutions, country of origin and topic of article. This analysis is done with the view to analysing the changes that have occurred and the topics that are emerging in the journal.

Findings
Whilst initially a journal focussed more on European entrepreneurship research, there has been an increased internationalisation of the topics and countries represented in the journal. In addition, initially the focus was more on small business but this has widened to include more diverse topics related to entrepreneurship such as learning.

Originality/value
As evident in the bibliometric analysis and growth of citations, this journal has published innovative and path breaking studies that impact other journals. Thus, it is important to analyse how the journal has grown and what the future holds.
Design, dynamic modelling and control of tilt-rotor UAVs: a review
Authors: Hegde Navya Thirumaleshwar , George V.I. , Nayak C. Gurudas , Kumar Kamlesh 
Published: September 2019

Purpose
The purpose of this paper is to give reviews on the platform modeling and design of a controller for autonomous vertical take-off and landing (VTOL) tilt rotor hybrid unmanned aerial vehicles (UAVs). Nowadays, UAVs have experienced remarkable progress and can be classified into two main types, i.e. fixed-wing UAVs and VTOL UAVs. The mathematical model of tilt rotor UAV is time variant, multivariable and non-linear in nature. Solving and understanding these plant models is very complex. Developing a control algorithm to improve the performance and stability of a UAV is a challenging task.

Design/methodology/approach
This paper gives a thorough description on modeling of VTOL tilt rotor UAV from first principle theory. The review of the design of both linear and non-linear control algorithms are explained in detail. The robust flight controller for the six degrees of freedom UAV has been designed using H-infinity optimization with loop shaping under external wind and aerodynamic disturbances.

Findings
This review will act as a basis for the future work on modeling and control of VTOL tilt rotor UAV by the researchers. The development of self-guided and fully autonomous UAVs would result in reducing the risk to human life. Civil applications include inspection of rescue teams, terrain, coasts, border patrol buildings, police and pipelines. The simulation results show that the controller achieves robust stability, good adaptability and robust performance.

Originality/value
The review articles on quadrotors/quadcopters, hybrid UAVs can be found in many literature, but there are comparatively a lesser amount of review articles on the detailed description of VTOL Tilt rotor UAV. In this paper modeling, platform design and control algorithms for the tilt rotor are presented. A robust H-infinity loop shaping controller in the presence of disturbances is designed for VTOL UAV.
Lean manufacturing and productivity changes: the moderating role of R&D
Authors: Shi Yingjie , Wang Xinyu , Zhu Xuechang 
Published: September 2019

PurposeThe purpose of this paper is to empirically investigate the effect of lean manufacturing on productivity changes and to identify the root sources of productivity changes. Furthermore, the authors explore the moderating effects of research and development (R&D) to examine the relationship between lean manufacturing and productivity changes.Design/methodology/approachThis paper employs the propensity score matching (PSM) model combined with the difference-in-difference (DID) estimation to overcome the selectivity bias. The Malmquist productivity index is used to capture productivity changes. By analyzing 671 Chinese manufacturing listed firms from 2009 to 2014, the moderating effects of R&D on the relationship between lean manufacturing and productivity changes are measured.FindingsThe results reveal that lean manufacturing implementation has non-significant effects on productivity changes in principle, while a detailed analysis indicates that lean manufacturing could improve scale efficiency significantly. While engaged in R&D could significantly improve the efficiency of technological changes for lean manufacturing implementation firms, there exist negative effects on pure technical efficiency.Research limitations/implicationsThis research only covers manufacturing listed firms in China. Further studies should extend the generalizability of the findings.Practical implicationsThis study helps managers to identify the important role of R&D on the relationship between lean manufacturing and productivity changes and provides insights into how to improve the lean manufacturing performance.Originality/valueThis paper appears to be one of the earliest studies on the relationship between lean manufacturing and productivity changes by applying the PSM combined with DID estimation in Chinese manufacturing environment.
An empirical analysis of emoji usage on Twitter
Authors: Li Mengdi , Chng Eugene , Chong Alain Yee Loong , See Simon 
Published: September 2019

Purpose
Emoji has become an essential component of any digital communication and its importance can be attested to by its sustained popularity and widespread use. However, research in Emojis is rarely to be seen due to the lack of data at a greater scale. The purpose of this paper is to systematically analyse and compare the usage of Emojis in a cross-cultural manner.

Design/methodology/approach
This research conducted an empirical analysis using a large-scale, cross-regional emoji usage data set from Twitter, a platform where the limited 140 characters allowance has made it essential for the inclusion of emojis within tweets. The extremely large textual data set covers a period of only two months, but the 673m tweets authored by more than 2,081,542 unique users is a sufficiently large sample for the authors to yield significant results.

Findings
This research discovered that the categories and frequencies of Emojis communicated by users can provide a rich source of data to understand cultural differences between Twitter users from a large range of demographics. This research subsequently demonstrated the preferential use of Emojis complies with Hofstede’s Cultural Dimensions Model, in which different representations of demographics and culture within countries present significantly different use of Emojis to communicate emotions.

Originality/value
This study provides a robust example of how to strategically conduct research using large-scale emoji data to pursue research questions previously difficult. To the best of authors’ knowledge, the present study pioneers the first systematic analysis and comparison of the usage of emojis on Twitter across different cultures; it is the largest, in terms of the scale study of emoji usage to-date.
The influence of ESG information on investment allocation decisions
Authors: Khemir Souhir , Baccouche Chedli , Ayadi Salma Damak 
Published: September 2019

PurposeIn addition to financial reporting, more and more companies report environmental, social and governance (ESG) information in emerging countries. This practice is intended to fulfill the information needs of all the company’s stakeholders, and more specifically the investors. The purpose of this paper is twofold. First, to analyze whether investors include ESG information into their investment allocation decisions in Tunisian capital market. Second, to identify the information dimension having the more effect on their investment allocation decisions.Design/methodology/approachA field experiment was conducted in an emerging country (Tunisia) among 245 novices and experienced financial stakeholders to analyze how ESG information is taken into account in their investment allocation decisions.FindingsThe results of the factorial mixed analysis of variance show that ESG information influenced the investment allocation decisions in Tunisia. In addition, the results of thepost-hoctest indicate that governance and social information had more influence than environmental information.Research limitations/implicationsThis paper is limited to the analysis of the influence of ESG information only on the decisions of financial stakeholders in Tunisia. In future research works, it will be relevant to study the decisions of other stakeholders and to carry out comparative studies between several countries.Practical implicationsThe results can only strengthen and motivate companies to pay more attention to their ESG information disclosure practices. They are also likely to attract the attention of the accounting standard setters on the need to standardize these practices.Originality/valueThe original contribution of this paper lies not only in the analysis of three dimensions of extra-financial information: E, S and G through an experiment carried out in an emerging country, but also especially in the comparison of the influence of each dimension on investment allocation decisions.
Intensifying business success of Malaysian housing development firms through entrepreneurial learning
Authors: Syed Abu Bakar Syed Putra , Jaafar Mastura , Muhibudin Masitah 
Published: September 2019

Purpose
The purpose of this paper is to investigate the impacts of entrepreneurial learning on the performance of Malaysian private housing developers. Although the extant literature on learning epistemology is quite voluminous, the focus within the housing entrepreneurship remains limited. With this lacuna, the investigation on the perceptions of learning propensity among residential developers is deemed timely.

Design/methodology/approach
Using snowball sampling, interviews were conducted with ten perceived successful private housing developers in Malaysia to understand the antecedents and consequences of entrepreneurial learning in steering their business toward excellence.

Findings
The research outcomes revealed that all the interviewed respondents concurred with the importance of entrepreneurial learning within the organization to strive for business success. The interviewed housing developers highlighted the significance of learning interest in their daily works to achieve business competitiveness and gain competitive edge against rivals. Through continuous learning, positive ideas and ethos could be instilled within the uncertain business environment.

Research limitations/implications
From an empirical standpoint, the sample was limited to private housing developers in one country. This limits the scope of generalization. Further studies, comparing between public and private developers in multiple contexts should be undertaken. Besides that, the research is based on a relatively small sample of ten respondents and the use of snowball sampling may have meant that the respondents would only tend to present a particular view of the industry.

Practical implications
The results raise interesting impacts on business success for scholars and entrepreneurs to ponder upon. The research contributes new knowledge about the effects of entrepreneurial learning toward the competitiveness of housing developers, by looking at the whole aspects of development process. A better understanding on how business knowledge could be retrieved from the perspectives of Malaysian housing developers is posited to be pragmatically employed.

Originality/value
Concomitantly with the above proposition, the study offers a novel insight into learning capabilities of Malaysian private housing developers that may be useful for global references. Understanding firm’s resources and capabilities is emergent that warrants further clarifications on entrepreneurial learning within housing development firms. The paper successfully illuminates comprehensive issues that have responded to this shortfall.
Techno-economic assessment of using wind power system for tribal region of Gachsaran in Iran
Authors: Mostafaeipour Ali , Qolipour Mojtaba , Rezaei Mostafa , Goudarzi Hossein 
Published: September 2019

Purpose
This paper aims to investigate the techno-economic feasibility of wind power potential for a tribal region located in Gachsaran in the South-West of Iran.

Design/methodology/approach
Techno-economic feasibility study and analysis of data were conducted by HOMER v2.68 software. Simulations and calculations were performed for 10 kW turbines, 8 Trojan L16P batteries, 12 kW converter and 12 kW generator. To anticipate the pay back period (PBP) or the time required to reach profitability, an engineering economic method named net equivalent uniform annual was applied.

Findings
The power plant construction cost, including the initial cost, installing, replacing and project operating costs for useful life of 20 years was equal to $40970. The net income of the project for each year was $8538 and the calculations were carried out using interest rate of 18%. Results indicated that PBP was 13 years which is lower than 20 years useful life of the turbine. Therefore, it is economically feasible to use this type of turbine for the nominated region.

Originality/value
There has not been conducted a research regarding remote areas in Iran; therefore, this study aims at closing this research gap. Moreover, this method could be used for any remote areas in any other developing country.