bluuebunny/crossref_metadata_2025_split huggingface dataset:
New data on spermiogenesis and trepaxonematan axoneme in basal tapeworms (Cestoda, Caryophyllidea, Lytocestidae) parasitizing cyprinid fishes
Authors: Matoušková Martina , Bílý Tomáš , Bruňanská Magdaléna , Oros Mikuláš , Kostič Borislav , Nebesářová Jana 
Published: September 2019

AbstractMonozoic caryophyllidean cestodes, intestinal parasites of cyprinid fishes, represent a group of tapeworms with an unclear evolutionary history. As spermatology may provide phylogenetically important data, the spermiogenesis and ultrastructure of the mature spermatozoon have been investigated using an integrative approach combining transmission electron microscopy, cytochemistry and electron tomography in Khawia rossittensis (Szidat, 1937). The process of spermatid formation is accompanied by the presence of ultrastructural characters not described in traditional models of spermiogenesis, e.g., apical electron-dense material, the two striated roots situated unusually opposite each other, branching of typical striated roots, an intercentriolar body comprising five electron-dense and four electron-lucent layers, rotation of both free flagella and flagellar buds to the median cytoplasmic process at 90°, and a complete proximodistal fusion. The synchronous rotation of both flagellar buds and growing free flagella is an evolutionarily linked pattern favouring the hypothesis that the Caryophyllidea are not ancestral but are secondarily derived from polyzoic forms. Electron tomography analysis has revealed a unique feature of two helicoidal tubular structures in the central electron-dense core of the axoneme of mature spermatozoon. These data provide new insights into the architecture of the 9 + ‘1’ axoneme, which is shared by male gametes of all trepaxonematan Platyhelminthes.
NaV1.2 haploinsufficiency in Scn2a knock-out mice causes an autistic-like phenotype attenuated with age
Authors: Léna Isabelle , Mantegazza Massimo 
Published: September 2019

AbstractMutations of the SCN2A gene, encoding the voltage gated sodium channel NaV1.2, have been associated to a wide spectrum of epileptic disorders ranging from benign familial neonatal-infantile seizures to early onset epileptic encephalopathies such as Ohtahara syndrome. These phenotypes may be caused by either gain-of-function or loss-of-function mutations. More recently, loss-of-function SCN2A mutations have also been identified in patients with autism spectrum disorder (ASD) without overt epileptic phenotypes. Heterozygous Scn2a knock-out mice (Scn2a+/−) may be a model of this phenotype. Because ASD develops in childhood, we performed a detailed behavioral characterization of Scn2a+/− mice comparing the juvenile/adolescent period of development and adulthood. We used tasks relevant to ASD and the different comorbidities frequently found in this disorder, such as anxiety or intellectual disability. Our data demonstrate that young Scn2a+/− mice display autistic-like phenotype associated to impaired memory and reduced reactivity to stressful stimuli. Interestingly, these dysfunctions are attenuated with age since adult mice show only communicative deficits. Considering the clinical data available on patients with loss-of-function SCN2A mutations, our results indicate that Scn2a+/− mice constitute an ASD model with construct and face validity during the juvenile/adolescent period of development. However, more information about the clinical features of adult carriers of SCN2A mutations is needed to evaluate comparatively the phenotype of adult Scn2a+/− mice.
A first experience of transduction for differentiated HepaRG cells using lentiviral technology
Authors: Pivert Adeline , Lefeuvre Caroline , Tran Cong-Tri , Baillou Claude , Durantel David , Le Guillou-Guillemette Hélène , Lemoine François M. , Lunel-Fabiani Françoise , Ducancelle Alexandra 
Published: September 2019

AbstractCurrently, there is a lack of systems for studying the role of hepatitis B viral proteins, such as HBeAg and HBcAg, on liver injury. It is necessary to develop an original tool in order to clarify the role of these viral proteins in hepatic stellate cell activation, and to understand the molecular mechanisms of liver injury. HepaRG are the most reliable hepatocyte-like cells for studying liver functions or disorders. In this paper, we demonstrate that the transduction of differentiated HepaRG (dHepaRG) cells can be performed successfully using lentiviral particles. The production of a functional Green Fluorescent Protein (GFP) assessed by Fluorescence Activated Cell Sorting and fluorescence microscopy is up to 16% of GFP positive cells using a multiplicity of infection (MOI) of 2.4. We demonstrate that this technology can allow the stable expression of GFP during the long lifecycle of the cell (up to four weeks after the cell’s passage). With this innovative tool, we aim to express viral proteins such as HBeAg or HBcAg in dHepaRG cells. The preliminary results of this work shows that HBeAg can be efficiently produced in dHepaRG cells and that increased MOI allows a better production of this protein. Our future objective will be to study the role of HBc and HBe proteins on the induction of hepatic fibrosis.
Laboratory and field evaluation of acetic acid-based lures for male Asian citrus psyllid, Diaphorina citri
Authors: Zanardi Odimar Z. , Volpe Haroldo X. L. , Luvizotto Rejane A. G. , Magnani Rodrigo F. , Gonzalez Francisco , Calvo Carolina , Oehlschlager Cameron A. , Lehan Benjamin J. , Esperança Victoria , Delfino Jennifer Y. , de Freitas Renato , de Carvalho Rômulo Igor , Mulinari Tatiana Aparecida , Miranda Marcelo P. , Bento José Mauricio S. , Leal Walter S. 
Published: September 2019

AbstractThe Asian citrus psyllid (ACP) is a vector of a pathogen associated with greening and thus a major problem in citriculture worldwide. Lures are much needed for improving ACP trapping systems for monitoring populations and surveillance. Previously, we have identified acetic acid as a putative sex pheromone and measured formic acid- and propionic acid-elicited robust electroantennographic responses. We have now thoroughly examined in indoor behavioral assays (4-way olfactometer) and field tests the feasibility of these three semiochemicals as potential lures for trapping ACP. Formic acid, acetic acid, and propionic acid at appropriate doses are male-specific attractants and suitable lures for ACP traps, but they do not act synergistically. An acetic acid-based homemade lure, prepared by impregnating the attractant in a polymer, was active for a day. A newly developed slow-release formulation had equal performance but lasted longer, thus leading to an important improvement in ACP trap capture at low population densities.
Alpha-tocopherol fertigation confers growth physio-biochemical and qualitative yield enhancement in field grown water deficit wheat (Triticum aestivum L.)
Authors: Ali Qasim , Ali Shafaqat , Iqbal Naeem , Javed Muhammad Tariq , Rizwan Muhammad , Khaliq Roubina , Shahid Sumreena , Perveen Rashida , Alamri Saud A. , Alyemeni Mohammed Nasser , Wijaya Leonard , Ahmad Parvaiz 
Published: September 2019

AbstractWater stress is a major problem to fulfill the world food demand and to solve the problem of malnutrition. Different strategies are being used to solve these problems including the fertigation of plants with different biochemical at different growth stages. The present study was conducted for the induction of drought tolerance in field grown wheat for better yield and nutritional quality through foliar spray of α-tocopherol (α-Toc) at start of reproductive stage. Water stress was maintained based on number of irrigation. Three levels of α-Toc 0.001, 0.01 and 0.1 mM were applied as foliar spray. Water stress significantly reduced the biomass production that associated with the decreased photosynthetic pigments, water relation, photosynthetic efficiency, but increased the lipid peroxidation, leaf relative membrane permeability, activities of antioxidant enzymes and the contents of phenolic, flavonoids, α-toc and ASA. Water stress also negatively effected the different yield attributes and seed nutrient quality. Foliar fertigation of wheat plants with α-Toc significantly improved the water stress tolerance of wheat plants in term of improvement in growth and seed yield associated with improved water relations, photosynthetic efficiency, contents of photosynthetic pigments and improvement in antioxidative defence mechanism (enzymatic and non-enzymatic antioxidants). Fertigation of water stressed wheat plants with α-Toc also improved the seed nutritional quality in terms of the contents of seed phenolics, flavonoids, activities of antioxidant enzymes and the content of α-, β- and γ-tocopherols. In conclusion, it was found that fertigation of water stressed wheat plants not only improved the water stress tolerance but also improved the seed yield and nutritional quality that will not only be helpful for the improvement in wheat yield that also be a step to solve the problem of malnutrition through the bio-fertification of α-Toc.
A versatile platform technology for recombinant vaccines using non-propagative human parainfluenza virus type 2 vector
Authors: Ohtsuka Junpei , Fukumura Masayuki , Furuyama Wakako , Wang Shujie , Hara Kenichiro , Maeda Mitsuyo , Tsurudome Masato , Miyamoto Hiroko , Kaito Aika , Tsuda Nobuyuki , Kataoka Yosky , Mizoguchi Akira , Takada Ayato , Nosaka Tetsuya 
Published: September 2019

AbstractEctopic protein with proper steric structure was efficiently loaded onto the envelope of the F gene-defective BC-PIV vector derived from human parainfluenza virus type 2 (hPIV2) by a reverse genetics method of recombinant virus production. Further, ectopic antigenic peptide was successfully loaded either outside, inside, or at both sides of the envelope of the vector. The BC-PIV vector harboring the Ebola virus GP gene was able to elicit neutralizing antibodies in mice. In addition, BC-PIV with antigenic epitopes of both melanoma gp100 and WT1 tumor antigen induced a CD8+ T-cell-mediated response in tumor-transplanted syngeneic mice. Considering the low pathogenicity and recurrent infections of parental hPIV2, BC-PIV can be used as a versatile vector with high safety for recombinant vaccine development, addressing unmet medical needs.
Patientenzufriedenheit nach Lokalanästhesie bei ambulanter
Kehlkopf-Operation
Authors: Grundmann Thomas 
Published: September 2019

Naunheim MR and Woo P. Topical Anesthetic Techniques in Office-Based Laryngeal
Surgery: A Comparison of Patient Preferences. Laryngoscope, 00:1–5, 2019; doi:
10.1002/lary.27894
Bei ambulanten Kehlkopfoperationen gibt es verschiedene Techniken für die
Lokalanästhesie. Bisher besteht jedoch keine Einigkeit über die optimale
Applikationsform des Betäubungsmittels. Im Rahmen dieser Studie verglichen
die Autoren drei verschiedene Anästhesietechniken und bewerteten die
Zufriedenheit von Patienten und Operateur.
Die kontralaterale doppelte
Transpositionslappenplastik
Authors: Konstantinow A. , Arnold A. 
Published: September 2019

ZusammenfassungDas Prinzip der einfachen Transpositionslappenplastik (TLP) (Synonym:
Schwenklappenplastik) besteht darin, dass in der unmittelbaren Umgebung eines
Hautdefektes ein gestielter Lappen mit axialer oder randomisierter
Gefäßversorgung generiert und durch eine geringfügige Drehbewegung an seiner
Basis über eine dazwischenliegende Zone von normaler Haut in den Defekt
eingeschwenkt wird. Häufig erwähnte Varianten der einfachen
Transpositionslappenplastik sind die Rhomboidplastiken nach Limberg und
Dufourmentel sowie die unilateralen „bi-lobed“ und „tri-lobed“ Schwenklappen
1, 2,
3, 4,
5, 6.
Die kontralaterale doppelte TLP findet hingegen in der Literatur nur sehr selten
Erwähnung 4.
Arzt-Bewertungsportale – Tipps und Tricks
Authors: Koch U. 
Published: September 2019

ZusammenfassungWenn die ärztliche Tätigkeit und alle sonstigen Eigenschaften der eigenen
Behandlungseinrichtung öffentlich im Internet bewertet werden, empfinden viele
Ärzte dies als unbequem, ehrenrührig oder sehen gar ein Risiko für den
wirtschaftlichen Erfolg. Die Rechtsprechung in Deutschland ist eindeutig: Es
gibt keine Vermeidungsstrategie. Dieser Beitrag führt einige Handlungsoptionen
für Ärzte auf, die sich aktiv mit Online-Bewertungen auseinandersetzen wollen
und bewertet diese Optionen anhand eigener Erfahrungen des Autors.
External Validation of the GREAT Score in Turkish Patients with Gravesʼ Hyperthyroidism Treated with the Titration Regimen Method of Antithyroid Drugs: A Multicenter Study
Authors: Zuhur Sayid Shafi , Elbuken Gulsah , Yildiz Ismail , Kadioglu Pinar , Erol Selvinaz , Sahin Serdar , Kilinc Faruk , Akbaba Gulhan , Topcu Birol , Altuntas Yuksel 
Published: September 2019

AbstractRecently, the Gravesʼ Recurrent Events After Therapy score (GREAT) was proposed as a useful tool to predict relapse before starting antithyroid drugs (ATD) in patients with Gravesʼ disease (GD). Therefore, we intended to assess the validity of the GREAT score in Turkish patients with GD, including patients who experienced a poorly controlled disease (multiple episodes of hyperthyroidism followed by euthyroidism or rarely hypothyroidism) during ATD dose titration. This is a retrospective multicenter study including 517 patients with the first episode of GD who were treated for at least 12 months. The patients were classified as relapse+poorly controlled disease (non-remission) and remission groups. During a median follow-up time of 35 months (12–144 months), 191 (37%) patients experienced a relapse, 136 (26.3%) a poorly controlled disease, and 190 (36.7%) remained in remission. Patients with non-remission disease tended to have significantly higher serum levels of TRAb, fT4, and fT3, and have larger goiter sizes on palpation at baseline, as compared with the remission group. Non-remission disease occurred in 12, 35, and, 53% of the patients falling into GREAT class I, II, and III, respectively (hazard ratio 2.56, 95% CI 2.02–3.51, p=0.012, and hazard ratio 3.54, 95% CI 2.12–5.91, p<0.001, for GREAT class II and III against class I, respectively). According to our study, the GREAT score is a useful tool to predict the risk of relapse as well as the occurrence of poorly controlled disease before starting treatment with ATDs.
Go with the (Lymph) Flow?
Authors: Mortimer Peter 
Published: September 2019

My inspiration for a career in lymphatics came from two sources: the first was a visit to the Foeldi clinic and the second was a visit to Professor Fredi Bollinger’s angiology lab in Zurich. These back to back visits in 1984 gave me insight into the best practice in lymphoedema treatment at the time as well as state of the art research techniques. On my return, I unashamedly introduced the Foeldi principles of lymphoedema treatment to the UK. I also set about setting up Fluorescence microlymphangiography in my new lab. Also present during my visit to Zurich was Hugo Partsch who, as a fellow dermatologist, has been very influential ever since as a role model, mentor and friend.The idiom ‘go with the flow’ means go with the prevailing attitude or do what the majority are doing even if you disagree. There is an implication one does not really believe it is necessarily the right thing to do but it is the easiest thing to do. There is a tendency currently with interstitial fluid dynamics to ‘go with the flow’ despite overwhelming scientific evidence that the accepted direction of flow is actually incorrect. As a result, the lymphatic system has been underestimated in its importance for the understanding and treatment of peripheral oedema.
Ex Vivo Comparison of the Accuracy of a Clamped with a Hand-Held Drill Guide for Drilling Distal Third Metacarpals and Metatarsals in Equids
Authors: James Oliver A. , Elce Yvonne , Cillán-Garcia Eugenio , Reardon Richard J.M. 
Published: September 2019

Objectives The aim of this study was to determine whether a clamped drill guide can be used effectively to drill across equine distal third metacarpals and metatarsals and to compare accuracy and speed of a drilling with a hand-held drill guide.
Study Design Eighty equine cadaver limbs were divided between four surgeons with varying experience. The limbs were randomly allocated to clamped or hand-held drill guides and placed in a stand in an upright/standing position on a metal table. Drilling times from cis- to trans cortices were measured. Post-drilling computed tomography images were used to assess drilling deviations in the dorsopalmar and proximodistal directions.
Results Mean proximodistal deviation of the drill hole was distad for both guides and significantly (p = 0.01) less for the clamped drill guide +0.35° (range: –5.42°, +6.61°, standard deviation [SD] ± 3.18°) than the hand-held drill guide +2.05° (range: –5.24°, +9.81°, SD ± 3.69°). Mean dorsopalmar/plantar deviation was non-significantly (p = 0.17) less for the clamped drill guide −0.27° (range: −9.72°, +6.58°, SD ± 3.57°) than the hand-held drill guide +0.60° (range: −9.74°, + 12.33°, SD ± 4.51°). Mean time taken to drill from cis to trans cortex was significantly (p < 0.001) shorter with the clamped drill guide (41.2s) than the hand-held guide (56.6s).
Conclusion The clamped drill guide could be used successfully on the equine distal third metacarpals and metatarsals and resulted in some improved accuracy and reduced drilling time compared with the hand-held guide. The use of the clamped drill guide in vivo could be supported. This may be of benefit for certain fracture repairs in equids.
Steroid Therapy in the Management of Sudden Sensorineural Hearing Loss: A Nonrandomized Clinical Trial
Authors: Swaminathan Gowri , Ramalingam Ravi , Kombupalayam Kumarappa Gounder Ramalingam 
Published: September 2019

AbstractObjective The aim of this study was to compare the effectiveness of intratympanic (IT) steroid therapy and combined intravenous-cum-intratympanic (IVIT) steroid therapy in the management of sudden sensorineural hearing loss (SSNHL).Study Design This was a nonrandomized clinical trial.Setting Tertiary referral center.Patients Forty-four patients, who presented to the outpatient department or the emergency room and those who fulfilled the inclusion criteria, were enrolled in this study. The patients were put into two groups: Group IT—that received intratympanic steroid therapy alone and Group IVIT—that received intratympanic as well as intravenous steroid therapies.Intervention Therapeutic—in the form of IVIT steroid therapy.Main Outcome Measure Impact of the steroid therapy in patients with SSNHL was measured objectively with the help of pure tone audiometry (PTA). The improvement in hearing was assessed in terms of decibels gained after the intervention in both the groups.Results The mean improvement in PTA after 3 months in Group IT was 19.78 (±18.918) dB, whereas the mean improvement after 3 months in Group IVIT was 22.29 (±16.147) dB, statistically showing no significant difference between the groups.Conclusion The authors recommend the use of IT steroid therapy alone in the management of SSNHL.
The Differing Effect of Gender and Clinical Specialty on Physicians' Intention to Use Electronic Medical Record
Authors: Hwang Hsin-Ginn , Dutta Bireswar , Chang Hui-chuan 
Published: September 2019

Abstract
Background The use of electronic medical record (EMR) is anticipated to bring benefits for patients, physicians, and organizations. But limited physicians' acceptance of EMR presents a serious threat to its effective implementation.
Objectives The current study incorporates technology acceptance model (TAM) with two antecedents, gender, and clinical specialty and one context-specific factor, financial incentives, to identify the factors that influence physicians' intention to use EMR in Taiwan.
Methods The survey methodology was used to collect data from the physicians, working in the regional hospital that had implemented EMR system. A total of 119 out of 213 questionnaires returned in a response rate of 56%. But four responses were considered ineffective due to missing values. The structural equation modeling (SEM) technique was employed to analyze the research framework.
Results The partial least squares (PLS) regression indicated that three factors perceived usefulness, financial incentives, and attitude toward using EMR significantly affect physicians' intention. But concerning perceived ease of use (PEOU), an insignificant path coefficient was reported. Additionally, regression analysis showed gender, and clinical specialty positively influenced physicians' intention to use EMR.
Discussion and Conclusions The proposed research framework contributes to the conclusive explanation for interpreting physicians' intention to use EMR. Physicians generally have a higher level of computer literacy. Therefore, the factor of PEOU could not be critical regarding adopting new health information technology (HIT). This study also brings perspectives from the gender, and clinical differences have primarily been missing in the literature of the physicians' intention to use HIT. In doing so, it infers how gender, and clinical specialty, may complement (and in some instances, reinforce) the influence of technological and attitudinal factors of HIT use. Thus, health care providers must take these factors into consideration in the development and validation of the theories regarding the intention to use EMR.
Revision of a Medial UKA to a Kinematic Aligned TKA: Comparison of Operative Complexity, Postoperative Alignment, and Outcome Scores to a Primary TKA
Authors: Shelton Trevor J. , Gill Manpreet , Athwal Gurbir , Howell Stephen M. , Hull Maury L. 
Published: September 2019

AbstractRevision of a medial unicompartmental knee arthroplasty (UKA) to a mechanically aligned total knee arthroplasty (MA TKA) is inferior to a primary TKA; however, revision with kinematic alignment (KA) has not been well studied. The present study determined whether patients revised with KA had a higher use of revision components, different postoperative alignment, and different clinical outcome scores from patients with a primary KA TKA. From 2006 to 2017, all patients suitable for a revision of a failed medial UKA to a TKA and a primary TKA were treated with KA. Reasons for the revision performed in ten females and six males at a mean age 67 ± 8 years included progression of osteoarthritis in the lateral hemi-joint (n = 6), aseptic loosening (n = 4), unremitting medial pain without loosening (n = 4), and insert wear (n = 2). Patients with a revision were matched 1:3 with a control cohort treated with a primary KA TKA. Revisions were performed with primary components without augments, stem extensions, or bone grafts. Seven postoperative alignment parameters of the limb and components were comparable to the control cohort (p > 0.05). At a mean follow-up of 5 years (1–10), implant survival was 100%, and the revision/primary group clinical outcome scores were 39/43 points for the Oxford Knee Score (OKS), 2.2/1.0 cm for the Visual Analog Pain Score, and 12/7 points for the Western Ontario and McMaster Universities Osteoarthritis Index score. When compared with primary KA TKA, surgeons that revise a failed medial UKA to a TKA with use of KA can expect similar operative complexity, comparable postoperative alignments, and a mean OKS of 39 points, which is higher than the mean 27 to 30 point range reported for revision of a failed UKA to a TKA with the use of MA.