bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recovery of blood flow regulation in microvascular resistance networks during regeneration of mouse gluteus maximus muscle
Authors: Fernando Charmain A. , Pangan Aaron M. , Cornelison DDW , Segal Steven S. 
Published: December 2018

Key points
Skeletal muscle regenerates after injury, however the recovery of its microvascular supply is poorly understood. We injured the gluteus maximus muscle in mice aiming to investigate the recovery of blood flow regulation in microvascular resistance networks. We hypothesized that blood flow regulation recovers in concert with myofibre regeneration.
Microvascular perfusion ceased within 1 day post injury and was restored at 5 days coincident with the appearance of new myofibres; however, the resistance network was dilated and unresponsive to vasoactive agents. Spontaneous vasomotor tone, endothelium‐dependent dilatation and adrenergic vasoconstriction increased at 10 days in concert with myofibre regeneration. Vasomotor control recovered at 21 days, when regenerated myofibres matured and active force production stabilized. Functional vasodilatation in response to muscle contraction recovered at 35 days.
Physiological integrity of microvascular smooth muscle and endothelium recovers in parallel with myofibre regeneration. Additional time is required to restore the efficacy of signalling between myofibres and microvascular networks controlling their oxygen supply.
AbstractMyofibre regeneration after skeletal muscle injury is well‐studied, although little is known about how microvascular perfusion is restored. The present study aimed to evaluate the recovery of blood flow regulation during skeletal muscle regeneration. In anaesthetized male C57BL/6J mice (aged 4 months), the gluteus maximus muscle (GM) was injured by local injection of barium chloride solution (1.2%, 75 μL). Functional integrity of the resistance network was evaluated at 5, 10, 21 and 35 days post‐injury vs. Control by measuring internal diameter of feed arteries, first‐, second‐ and third‐order arterioles supplying the GM using intravital microscopy. The resting diameters of all branch orders were significantly greater (P < 0.05) than Control at 5 and 10 days and recovered to Control by 21 days, as did spontaneous vasomotor tone. Vasodilatation to ACh and vasoconstriction to phenylephrine (10−9 to 10−5 m) were absent at 5 days, increased at 10 days and recovered to Control by 21 days; reactivity improved in a distal‐to‐proximal gradient. Across branch orders, functional vasodilatation to single tetanic contraction (100 Hz, 500 ms) and to rhythmic twitch contractions (4 Hz, 30 s) was impaired at 5 days, improved through 21 days and was not different from Control at 35 days. Peak force development (g) was 60% of Control at 10 days and recovered by 21 days. Diminished vasomotor tone during the initial stages of regeneration promotes tissue perfusion as myofibre recovery begins. Recovery of tone and vasomotor responses to agonists occur in concert with myofibre regeneration. Delayed recovery of functional vasodilatation indicates that additional time is required to restore signalling between contracting myofibres and their vascular supply.
Efficiently Managing the Impact of Hardware Variability on GPUs’ Streaming Processors
Authors: Tan Jingweijia , Yan Kaige 
Published: December 2018

Graphics Processing Units (GPUs) are widely used in general-purpose high-performance computing fields due to their highly parallel architecture. In recent years, a new era with the nanometer scale integrated circuit manufacture process has come. As a consequence, GPUs’ computation capability gets even stronger. However, as process technology scales down, hardware variability, e.g., process variations (PVs) and negative bias temperature instability (NBTI), has a higher impact on the chip quality. The parallelism of GPU desires high consistency of hardware units on chip; otherwise, the worst unit will inevitably become the bottleneck. So the hardware variability becomes a pressing concern to further improve GPUs’ performance and lifetime, not only in integrated circuit fabrication, but more in GPU architecture design.
Streaming Processors (SPs) are the key units in GPUs, which perform most of parallel computing operations. Therefore, in this work, we focus on mitigating the impact of hardware variability in GPU SPs. We first model and analyze SPs’ performance variations under hardware variability. Then, we observe that both PV and NBTI have a large impact on SPs’ performance. We further observe unbalanced SP utilization, e.g., some SPs are idle when others are active, during program execution. Leveraging this observation, we propose a Hardware Variability-aware SPs’ Management policy (HVSM), which dynamically dispatches computation in appropriate SPs to balance the utilizations. In addition, we find that a large portion of compute operations are duplicate. We also propose an Operation Compression (OC) technique to minimize the unnecessary computations to further mitigate the hardware variability effects. Our experimental results show the combined HVSM and OC technique effectively reduces the impact of hardware variability, which can translate to 37% performance improvement or 18.3% lifetime extension for a GPU chip.
Ghrelin acylation by ghrelin-O-acyltransferase can occur in healthy part of oncological liver in humans
Authors: Germain Natacha , Cuenco Joyceline , Ling Yiin , Minnion James S. , Bageacu Serban , Grouselle Dominique , Estour Bruno , Galusca Bogdan 
Published: December 2018

Activation of ghrelin is controlled by the enzyme ghrelin- O-acyl transferase (GOAT). In humans, localization of this acylation is poorly understood. The aim of this study is to explore GOAT localization and activation in the human liver by evaluating both bioactive and non-bioactive ghrelin in the bloodstream entering and leaving the liver and to simultaneously evaluate GOAT mRNA expression in the liver. A healthy part of oncologic hepatic tissue collected from nine patients undergoing hepatectomy was used to evaluate GOAT mRNA expression by quantitative real-time polymerase chain reaction (RT-qPCR). Simultaneously, blood from the portal vein, the suprahepatic vein, the subclavicular vein, and the radial artery was also sampled to assay total and acylated ghrelin. Acylated ghrelin level was significantly increased in the suprahepatic vein compared with the portal vein level (385 ± 42 ng/ml vs. 268 ± 24 ng/ml, P = 0.04). Suprahepatic-to-portal vein ratio for acylated ghrelin (acylation ratio) is 1.4 ± 0.1. Mean expression of GOAT mRNA in the liver, expressed as 2−∆Ct·µg total RNA−1·1 µl of liver tissue−1 was at 0.042 ± 0.021 arbitrary units. GOAT mRNA expression in the liver was correlated with acylated-to-total ghrelin ratio in the suprahepatic vein ( P = 0.016, R = 0.75) and with the acylation liver ratio ( P = 0.05, R = 0.61). Blood concentration of acylated ghrelin was found significantly increased after its passage through the liver, suggesting that acylation can occur in the liver. RT-qPCR data confirmed the presence of GOAT in the liver, with a positive correlation between GOAT expression and acylated ghrelin liver ratio. This study strongly suggests that the liver is a site of ghrelin acylation in humans. NEW & NOTEWORTHY Although the activation of ghrelin by the enzyme ghrelin- O-acyl transferase (GOAT) is yet well demonstrated, its localization, especially in humans, remains poorly understood. We explored GOAT localization and activation in the human liver by simultaneously evaluating both bioactive and non-bioactive ghrelin in the bloodstream entering and leaving the liver and also GOAT mRNA expression in the liver. We therefore showed for the first time, to our knowledge, that GOAT localized in the liver is active and takes part in ghrelin activation.
Locating primary somatosensory cortex in human brain stimulation studies: experimental evidence
Authors: Holmes Nicholas Paul , Tamè Luigi , Beeching Paisley , Medford Mary , Rakova Mariyana , Stuart Alexander , Zeni Silvia 
Published: December 2018

Transcranial magnetic stimulation (TMS) over human primary somatosensory cortex (S1) does not produce immediate outputs. Researchers must therefore rely on indirect methods for TMS coil positioning. The “gold standard” is to use individual functional and structural magnetic resonance imaging (MRI) data, but the majority of studies don’t do this. The most common method to locate the hand area of S1 (S1-hand) is to move the coil posteriorly from the hand area of primary motor cortex (M1-hand). Yet, S1-hand is not directly posterior to M1-hand. We localized the index finger area of S1-hand (S1-index) experimentally in four ways. First, we reanalyzed functional MRI data from 20 participants who received vibrotactile stimulation to their 10 digits. Second, to assist the localization of S1-hand without MRI data, we constructed a probabilistic atlas of the central sulcus from 100 healthy adult MRIs and measured the likely scalp location of S1-index. Third, we conducted two experiments mapping the effects of TMS across the scalp on tactile discrimination performance. Fourth, we examined all available neuronavigation data from our laboratory on the scalp location of S1-index. Contrary to the prevailing method, and consistent with systematic review evidence, S1-index is close to the C3/C4 electroencephalography (EEG) electrode locations on the scalp, ~7–8 cm lateral to the vertex, and ~2 cm lateral and 0.5 cm posterior to the M1-hand scalp location. These results suggest that an immediate revision to the most commonly used heuristic to locate S1-hand is required. The results of many TMS studies of S1-hand need reassessment. NEW & NOTEWORTHY Noninvasive human brain stimulation requires indirect methods to target particular brain areas. Magnetic stimulation studies of human primary somatosensory cortex have used scalp-based heuristics to find the target, typically locating it 2 cm posterior to the motor cortex. We measured the scalp location of the hand area of primary somatosensory cortex and found that it is ~2 cm lateral to motor cortex. Our results suggest an immediate revision of the prevailing method is required.
Dynamics of synaptic extracellular field potentials in the nucleus laminaris of the barn owl
Authors: McColgan Thomas , Kuokkanen Paula T. , Carr Catherine E. , Kempter Richard 
Published: December 2018

Synaptic currents are frequently assumed to make a major contribution to the extracellular field potential (EFP). However, in any neuronal population, the explicit separation of synaptic sources from other contributions such as postsynaptic spikes remains a challenge. Here we take advantage of the simple organization of the barn owl nucleus laminaris (NL) in the auditory brain stem to isolate synaptic currents through the iontophoretic application of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-receptor antagonist 1,2,3,4-tetrahydro-6-nitro-2,3-dioxo-benzo[ f]quinoxaline-7-sulfonamide (NBQX). Responses to auditory stimulation show that the temporal dynamics of the evoked synaptic contributions to the EFP are consistent with synaptic short-term depression (STD). The estimated time constants of an STD model fitted to the data are similar to the fast time constants reported from in vitro experiments in the chick. Overall, the putative synaptic EFPs in the barn owl NL are significant but small (<1% change of the variance by NBQX). This result supports the hypothesis that the EFP in NL is generated mainly by axonal spikes, in contrast to most other neuronal systems. NEW & NOTEWORTHY Synaptic currents are assumed to make a major contribution to the extracellular field potential in the brain, but it is hard to directly isolate these synaptic components. Here we take advantage of the simple organization of the barn owl nucleus laminaris in the auditory brain stem to isolate synaptic currents through the iontophoretic application of a synaptic blocker. We show that the responses are consistent with a simple model of short-term synaptic depression.