bluuebunny/crossref_metadata_2025_split huggingface dataset:
SNPs in LncRNA genes are associated with non‐small cell lung cancer in a Chinese population
Authors: Wang Ruoyang , Feng Nannan , Wang Yu , Gao Sumeng , Zhang Fangfang , Qian Ying , Gao Ming , Yu Herbert , Zhou Baosen , Qian Biyun 
Published: April 2019

BackgroundIt has indicated that single nuclear polymorphisms (SNPs) in the regions encoding non‐coding transcripts are associated with lung cancer susceptibility. In a previous microarray study, we identified 13 differentially expressed long non‐coding RNAs (lncRNAs) in non‐small cell lung cancer (NSCLC) and associations of SNPs in these lncRNA genes with lung cancer were unknown. We conducted a case‐control study to address this issue.MethodsUsing the TaqMan method, we genotyped 17 SNPs located in the 13 lncRNA genes in 1294 cases with NSCLC and 1729 healthy controls. Unconditional logistic regression and Cox proportional hazards regression were used to analyze the associations of these SNPs with NSCLC risk and patient survival, respectively. These analyses were also repeated in subgroups of cases and controls stratified by gender, age group, smoking status, disease stage, and histological type.ResultsWe identified three SNPs associated with NSCLC risk. For SNP rs498238, CC genotype was associated with lower risk compared to TT genotype (adjusted OR = 0.33, 95%CI: 0.11‐0.97, P = 0.043). For rs16901995, CT/TT genotypes were associated with lower risk compared to CC genotype in non‐smokers (adjusted OR = 0.78, 95%CI: 0.62‐0.98, P = 0.035). Variant genotypes in rs219741 were associated with NSCLC risk in young patients, and the adjusted OR was 1.47 (95%CI: 1.03‐2.10, P = 0.033) when compared to the wild genotype. No SNPs were found to be associated with patient overall survival in the study.ConclusionThe study suggests that some genetic polymorphisms in the lncRNA genes may influence the risk of NSCLC among Chinese.
Changes in the sense of agency: Implications for the psychotherapy of bulimia nervosa‐ A case study
Authors: Kristmannsdottir Gudrun , Keski‐Rahkonen Anna , Kuusinen Kirsti‐Liisa 
Published: April 2019

AbstractA sense of agency is a transtheoretical concept that increases our understanding of important processes in psychotherapy. Agency can be described in terms of how strongly the person believes that she can have an impact on her problematic experiences and behaviors. In this case study, a patient's sense of agency in relation to symptoms of bulimia nervosa was assessed during 3 years of psychotherapy. Five distinct phases of agency in relation to eating disorder symptoms were identified: A false sense of agency or no agency at all, a weak sense of agency, a nascent sense of agency, a wavering sense of agency, and a strong sense of agency. A better understanding of patient agency can facilitate adapting approaches and methods best suited for the patient's capacity for change throughout treatment.
DGCR5 promotes cancer stem cell‐like properties of radioresistant laryngeal carcinoma cells by sponging miR‐506 via Wnt pathway
Authors: Tang Tian , Shan Guang 
Published: April 2019

AbstractCancer stem cells (CSCs) have been recognized as the significant cause of tumor recurrence. Long noncoding RNAs (lncRNAs) are involved in various cancers, including human laryngeal cancer. So far the correlation between lncRNA DiGeorge syndrome critical region gene 5 (DGCR5) and CSC‐like properties in human laryngeal cancer remains barely known. In our current study, two human larynx squamous carcinoma cell lines (Hep‐2 and Hep‐2R) with different radio sensitivities were cultured. Interestingly, CSC‐like phenotypes were much more enriched in Hep‐2R cells. We found that DGCR5 was upregulated and microRNA‐506 (miR‐506) was downregulated in Hep‐2R cells. In addition, silence of DGCR5 could inhibit the stemness and enhance the radiosensitivity of Hep‐2R cells. Meanwhile, overexpression of miR‐506 also suppressed the CSC‐like traits and the radiosensitivity was increased significantly. In addition, miR‐506 was predicted as target of DGCR5 and the correlation between them was validated in our study. Finally, we observed that Wnt pathway exerted a significant role in human laryngeal CSCs and DGCR5 inhibition could repress Wnt signaling activity by sponging miR‐506. In vivo assays were performed and we found that DCGR5 depressed stemness of human laryngeal cancer cells through modulating miR‐506 and Wnt signaling pathway. Taken these together, we reported that DGCR5 induced CSC‐like properties by sponging miR‐506 through activating Wnt in human laryngeal carcinoma cells.
Biliary atresia: a scientometric analysis of the global research architecture and scientific developments
Authors: Friedmacher Florian , Ford Kathryn , Davenport Mark 
Published: April 2019

AbstractBiliary atresia (BA) is a rare cholangiopathy of largely unknown etiology and unpredictable outcome. There has been an increasing number of BA‐related publications, which may challenge researchers to determine their actual scientific value. This study aimed to evaluate the global research activity and developments relating to BA using a combination of scientometric methodologies and visualization tools. A comprehensive search strategy for the Web of Science™ database was designed to obtain bibliographic data on scientific BA publications for the timespan 1900–2018. Research output of countries, institutions, individual authors and collaborative networks was analyzed. Semi‐qualitative research measures including citation rate and h‐index were assessed. Choropleth mapping and network diagrams were used to visualize results. In total, 4,459 publications on BA were identified (88.5% in English), originating from 63 countries. The largest number was published by the USA (n = 991; 22.2%), Japan (n = 667; 15.0%) and the UK (n = 294; 6.6%). The USA combined the highest number of cooperation articles (n = 140). The most productive collaborative network was established between the USA and Canada (n = 17). Scientific papers from the UK received the highest average citation rate (16.7), whereas the USA had the highest country‐specific h‐index (59). Eighty‐eight (2.0%) items were published under the auspices of multicenter consortiums and registries. The most productive institutions and authors were based in the USA, the UK, Japan, France, Canada and Taiwan. BA‐related research has constantly been progressing, becoming more multidisciplinary but with main research endeavors concentrated in a few high‐income countries. Studies into pathogenesis of BA remain uncommon, but are sorely needed to foster true scientific progress with this rare disease. Hence, international collaborative and translational research should be strengthened to allow further evolution in this field.
Targeted 64Cu‐labeled gold nanoparticles for dual imaging with positron emission tomography and optical imaging
Authors: Pretze Marc , van der Meulen Nick P. , Wängler Carmen , Schibli Roger , Wängler Björn 
Published: April 2019

Gold nanoparticles (AuNPs) have been used for many years in cancer treatment mainly for brachytherapy, but in the last 15 years, the focus has shifted to the development of ultrasmall target‐specific AuNPs with homogeneous size and, ultimately, tailored shapes for use in various imaging modalities such as computed tomography (CT), Raman, or photoacoustic imaging. Here, we report on the development of tumor‐specific AuNPs as diagnostic tools intended for the dual detection of prostate cancer via optical imaging (OI) and positron emission tomography (PET). The AuNPs were decorated with a near‐infrared dye and NODAGA chelator for complexation with radiometals. Radiolabeling with 64Cu was performed either indirect by complexation with NODAGA‐AuNPs or by direct reduction of [64Cu]Cu(0) onto the surface of the AuNPs. Both methods yielded stable 64Cu‐AuNPs with radiochemical yield more than 95% confirmed by HPLC. 64Cu‐AuNPs were evaluated in a dual‐imaging setting in vitro and in vivo and exhibited favorable diagnostic properties concerning detection, biodistribution, and clearance. Furthermore, the first therapeutic properties of the 64Cu‐AuNPs were evaluated in vitro concerning acute and long‐term toxicity, indicating that these 64Cu‐AuNPs could be used in therapeutic concepts in the future.
Proton density fat fraction MRI of vertebral bone marrow: Accuracy, repeatability, and reproducibility among readers, field strengths, and imaging platforms
Authors: Schmeel Frederic Carsten , Vomweg Toni , Träber Frank , Gerhards Arnd , Enkirch Simon Jonas , Faron Anton , Sprinkart Alois Martin , Schmeel Leonard Christopher , Luetkens Julian Alexander , Thomas Daniel , Kukuk Guido Matthias 
Published: April 2019

BackgroundChemical shift‐encoding based water‐fat MRI is an emerging method to noninvasively assess proton density fat fraction (PDFF), a promising quantitative imaging biomarker for estimating tissue fat concentration. However, in vivo validation of PDFF is still lacking for bone marrow applications.PurposeTo determine the accuracy and precision of MRI‐determined vertebral bone marrow PDFF among different readers and across different field strengths and imager manufacturers.Study TypeRepeatability/reproducibility.SubjectsTwenty‐four adult volunteers underwent lumbar spine MRI with one 1.5T and two different 3.0T MR scanners from two vendors on the same day.Field Strength/Sequence1.5T and 3.0T/3D spoiled‐gradient echo multipoint Dixon sequences.AssessmentTwo independent readers measured intravertebral PDFF for the three most central slices of the L1–5 vertebral bodies. Single‐voxel MR spectroscopy (MRS)‐determined PDFF served as the reference standard for PDFF estimation.Statistical TestsAccuracy and bias were assessed by Pearson correlation, linear regression analysis, and Bland–Altman plots. Repeatability and reproducibility were evaluated by Wilcoxon signed rank test, Friedman test, and coefficients of variation. Intraclass correlation coefficients were used to validate intra‐ and interreader as well as intraimager agreements.ResultsMRI‐based PDFF estimates of lumbar bone marrow were highly correlated (r2 = 0.899) and accurate (mean bias, –0.6%) against the MRS‐determined PDFF reference standard. PDFF showed high linearity (r2 = 0.972–0.978) and small mean bias (0.6–1.5%) with 95% limits of agreement within ±3.4% across field strengths, imaging platforms, and readers. Repeatability and reproducibility of PDFF were high, with the mean overall coefficient of variation being 0.86% and 2.77%, respectively. The overall intraclass correlation coefficient was 0.986 as a measure for an excellent interreader agreement.Data ConclusionMRI‐based quantification of vertebral bone marrow PDFF is highly accurate, repeatable, and reproducible among readers, field strengths, and MRI platforms, indicating its robustness as a quantitative imaging biomarker for multicentric studies.Level of Evidence: 3Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;50:1762–1772.
A predictive nomogram for individualized recurrence stratification of bladder cancer using multiparametric MRI and clinical risk factors
Authors: Xu Xiaopan , Wang Huanjun , Du Peng , Zhang Fan , Li Shurong , Zhang Zhongwei , Yuan Jing , Liang Zhengrong , Zhang Xi , Guo Yan , Liu Yang , Lu Hongbing 
Published: April 2019

BackgroundPreoperative prediction of bladder cancer (BCa) recurrence risk is critical for individualized clinical management of BCa patients.PurposeTo develop and validate a nomogram based on radiomics and clinical predictors for personalized prediction of the first 2 years (TFTY) recurrence risk.Study TypeRetrospective.PopulationPreoperative MRI datasets of 71 BCa patients (34 recurrent) were collected, and divided into training (n = 50) and validation cohorts (n = 21).Field Strength/Sequence3.0T MRI/T2‐weighted (T2W), multi‐b‐value diffusion‐weighted (DW), and dynamic contrast‐enhanced (DCE) sequences.AssessmentRadiomics features were extracted from the T2W, DW, apparent diffusion coefficient, and DCE images. A Rad_Score model was constructed using the support vector machine‐based recursive feature elimination approach and a logistic regression model. Combined with the important clinical factors, including age, gender, grade, and muscle‐invasive status (MIS) of the archived lesion, tumor size and number, surgery, and image signs like stalk and submucosal linear enhancement, a radiomics‐clinical nomogram was developed, and its performance was evaluated in the training and the validation cohorts. The potential clinical usefulness was analyzed by the decision curve.Statistical TestsUnivariate and multivariate analyses were performed to explore the independent predictors for BCa recurrence prediction.ResultsOf the 1872 features, the 32 with the highest area under the curve (AUC) of receiver operating characteristic were selected for the Rad_Score calculation. The nomogram developed by two independent predictors, MIS and Rad_Score, showed good performance in the training (accuracy 88%, AUC 0.915, P << 0.01) and validation cohorts (accuracy 80.95%, AUC 0.838, P = 0.009). The decision curve exhibited when the risk threshold was larger than 0.3, more benefit was observed by using the radiomics‐clinical nomogram than using the radiomics or clinical model alone.Data ConclusionThe proposed radiomics‐clinical nomogram has potential in the preoperative prediction of TFTY BCa recurrence.Level of Evidence: 3Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2019;50:1893–1904.
Influence of temporal parameters of DCE‐MRI on the quantification of heterogeneity in tumor vascularization
Authors: Crombé Amandine , Saut Olivier , Guigui Jerome , Italiano Antoine , Buy Xavier , Kind Michèle 
Published: April 2019

BackgroundEvaluating heterogeneity in tumor vascularization through texture analysis could improve predictions of patients' outcome and response evaluation.PurposeTo investigate the influence of temporal parameters on texture features extracted from dynamic contrast‐enhanced (DCE)‐MRI parametric maps.Study typeProspective cross‐sectional study.SubjectsTwenty‐five adults with soft‐tissue sarcoma (STS), median age: 68 years.Field Strength/SequenceDCE‐MRI acquisition using a CAIPIRINHA‐Dixon‐TWIST‐VIBE sequence at 1.5T (temporal resolutions: 2 sec, duration: 5 min).AssessmentThe area under time–intensity curve (AUC) and Ktrans maps were generated for several temporal resolution (dt = 2 sec, 4 sec, 6 sec, 8 sec, 10 sec, 12 sec, 20 sec) and scan durations (T = 3 min, 4 min, 5 min for a 6‐sec sampling) by downsampling and truncating the initial DCE‐MRI sequence. Tumor volume was manually segmented and propagated on all parametric maps. Thirty‐two first‐ and second order‐texture features were extracted per map to quantify the intratumoral heterogeneity.Statistical TestsThe influence of temporal parameters on texture features was studied with repeated‐measures analysis of variance (or nonparametric equivalent). The dispersion of each texture feature depending on temporal parameters was estimated with coefficients of variation (CVs). The performances of multivariate models to predict the response to chemotherapy (ie, binary logistic regression based on the baseline texture features) were compared.ResultsThe temporal resolution had a significant influence on 12/32 (37.5%) and 14/32 (43.8%) texture features evaluated on AUC and Ktrans maps, respectively (range of P < 0.0001–0.0395). Scan duration had a significant influence on 23/32 (71.9%) texture features from Ktrans map (range of P < 0.0001–0.0321). Dispersion was high (mean CV >0.5) with sampling for 2/32 (6.3%) and 10/32 (31.3%) features from AUC and Ktrans maps, respectively; and with truncating for 6/32 (18.8%) features from Ktrans map. The area under the receiver operating characteristics curve of predictive models ranged from 0.77 (95% confidence interval [CI] = [0.54–1.00], with dt = 6 sec T = 4 min) to 0.90 (95% CI = [0.74–1.00], with dt = 6 sec T = 5 min).Data ConclusionThe values of texture features extracted from DCE‐MRI parametric maps can be influenced by temporal parameters, which can lead to variations in performance of predictive models.Level of Evidence: 2Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2019;50:1773–1788.
Assigning the ESI mass spectra of organometallic and coordination compounds
Authors: McIndoe J. Scott , Vikse Krista L. 
Published: April 2019

AbstractElectrospray ionization mass spectrometry (ESI‐MS) is a useful technique for solving organometallic and coordination chemistry characterization problems that are difficult to address using traditional methods. However, assigning the ESI mass spectra of such compounds can be challenging, and the considerations involved in doing so are quite different from assigning the mass spectra of purely organic samples. This is a tutorial article for organometallic/coordination chemists using ESI‐MS to analyze pure compounds or reaction mixtures. The fundamentals of assigning ESI mass spectra are discussed within the context of organometallic and coordination systems. The types of ions commonly observed by ESI‐MS are categorized and described. Finally, a step‐by‐step guide for the assignment of organometallic and coordination chemistry ESI mass spectra is provided along with two case studies.
Synergy or substitution? The interactive effects of insiders' fairness and support and organizational socialization tactics on newcomer role clarity and social integration
Authors: Nasr Mohamed Ikram , El Akremi Assâad , Coyle‐Shapiro Jacqueline A.‐M. 
Published: April 2019

SummaryDrawing on fairness heuristics theory (Lind, 2001) and cue consistency theory (Maheswaran & Chaiken, 1991; Slovic, 1966), we test a moderated mediation model that examines whether the institutionalization of organizational socialization tactics enhances or constrains the beneficial effects of supervisory and coworker‐referenced justice and support on newcomer role clarity and social integration. The findings of a three‐wave study of 219 French newcomers show that although institutionalized tactics strengthen the positive indirect effects of supervisory interpersonal and informational justice on role clarity, via perceived supervisor support, it also acts as a substitute that weakens the positive indirect effect of coworker‐referenced interpersonal justice on social integration, via perceived coworker support. Implications of the findings for socialization research and practice are discussed.
Structural characterization of polysaccharide‐coated iron oxide nanoparticles produced by Staphylococcus warneri, isolated from a thermal spring
Authors: Kianpour Sedigheh , Ebrahiminezhad Alireza , Deyhimi Maryam , Negahdaripour Manica , Raee Mohammad Javad , Mohkam Milad , Rezaee Hamideh , Irajie Cambyz , Berenjian Aydin , Ghasemi Younes 
Published: April 2019

AbstractThe biocompatible‐coated iron oxide nanoparticles (IONs) have attracted a great interest because of their various applications in biological science and medicine. In most cases, the toxic effect of naked iron oxide nanoparticles is completely cleared by adding a biocompatible coating, such as polysaccharides, polyethylene glycol (PEG), or biosynthesis of biocompatible‐coated IONs using microorganisms such as bacteria. In the present study, polysaccharide‐coated iron oxide nanoparticles were produced by a strain of Staphylococcus warneri isolated from a thermal spring. For identification of the isolated bacterium, 16S rRNA gene sequencing was done. Characterization of the nanoparticles was performed for the first time, using transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), X‐ray crystallography (XRD), Fourier‐transform infrared (FTIR) spectroscopy, vibrating sample magnetometer (VSM), and 3‐(4,5‐dimethylthiazol‐2‐yl)−2,5‐diphenyltetrazolium bromide (MTT) assay. Results indicated that the spherical iron oxide nanoparticles were coated by a polysaccharide (13.6%), which provided a large negative charge of −91 mV and very low saturation magnetization of around 0.28 emu/g. The result of MTT assay on MOLT‐4 cell lines showed that the percentage of viability was between 95.6% and 68.9% in the 10–100 µM of nanoparticle concentrations with a high IC
50 value, which makes it appropriate for biomedical applications such as cancer therapy.
Methyl salicylate delays peel yellowing of ‘Zaosu’ pear (Pyrus bretschneideri) during storage by regulating chlorophyll metabolism and maintaining chloroplast ultrastructure
Authors: Zhang Huaiyu , Wang Rui , Wang Tian , Fang Chunxia , Wang Junjie 
Published: April 2019

AbstractBACKGROUNDIn some cultivars, yellowing resulting from chlorophyll breakdown has a direct and negative effect on food supply and health. The ‘Zaosu’ pear (Pyrus bretschneideri Rehd.), a commercial Asian pear cultivar in China, rapidly turns yellow when stored at room temperature after harvest. To develop techniques that delay or suppress chlorophyll degradation, the effects of methyl salicylate (MeSA) on yellowing in ‘Zaosu’ pear fruit during storage were evaluated.RESULTSCompared with the untreated fruit, the application of 0.05 mmol L−1 MeSA delayed the decline of the total chlorophyll, chlorophyll a and chlorophyll b content, and maintained more intact chloroplasts with fewer and smaller plastoglobuli. Methyl salicylate suppressed enzyme activities, including chlorophyllase, chlorophyll‐degrading peroxidase, Mg dechelatase, and pheophytinase, and the expression levels of NYC, NOL, CLH, SGR, PPH, PAO and RCCR in treated fruit.CONCLUSIONMethyl salicylate could delay chlorophyll breakdown in the fruit. The results also suggested that the conversion from chlorophyll a to pheophorbide a could proceed via two pathways, and that alternative pathways for the breakdown of chlorophyll a exist in ‘Zaosu’ pears. © 2019 Society of Chemical Industry
Pre‐harvest methyl jasmonate treatments increase antioxidant systems in lemon fruit without affecting yield or other fruit quality parameters
Authors: Serna‐Escolano Vicente , Valverde Juan M , García‐Pastor María E , Valero Daniel , Castillo Salvador , Guillén Fabián , Martínez‐Romero Domingo , Zapata Pedro J , Serrano Maria 
Published: April 2019

AbstractBACKGROUNDJasmonic acid (JA) and its volatile derivative methyl jasmonate (MeJA) are hormones involved in the regulation of many processes in plants and act (when applied as a post‐ or pre‐harvest treatment) to increase fruit bioactive compounds with antioxidant potential. However, there is no literature available regarding the effect of pre‐harvest MeJA treatment on lemon fruit antioxidant systems, which was the aim of the present study.RESULTSMeJA treatment (0.1, 0.5 and 1.0 mmol L–1) increased antioxidant compounds, such as phenolics, in the juice and flavedo of ‘Fino’ and ‘Verna’ lemons at harvest, with the most effective concentration being 0.1 mmol L–1 in both cultivars. In addition, catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) activities were also increased by MeJA treatment, with the highest increases being also found with 0.1 mmol L–1. The increases in APX and CAT were maintained from one treatment to another during fruit development on the tree, whereas the increase on POD disappeared after 8–10 days of each treatment. For both antioxidant systems, the highest increases were found in lemon harvested at the commercial ripening stage. By contrast, crop yield, fruit ripening process and quality parameters were generally not affected by MeJA treatment.CONCLUSIONPreharvest MeJA treatment could be a useful tool for increasing antioxidant potential and the health beneficial effects of lemon fruit consumption, given the relationship between these properties and phenolic content. Moreover, the increased concentration of phenolics and the activity of antioxidant enzymes in the flavedo of MeJA treated fruit could increase lemon tolerance to chilling injury and decay during postharvest storage. © 2019 Society of Chemical Industry