bluuebunny/crossref_metadata_2025_split huggingface dataset:
A potent small molecule, AS2863619, enhances the osteogenic potential and ameliorates the inflammation-induced impairment of osteogenesis in periodontal ligament stem cells
Authors: He Haipeng , Li Dongcai , Huang Jiamin , Cheng Baohui , Yang Dunhui , Yuan Jingyi , Yuan Xueshun , Zhao Meizhen , Zeng Xianhai , Li Quanli , Liu Zhiqiang , Hu Tianyong 
Published: November 2024

Abstract

Background Periodontal ligament stem cells (PDLSCs) represent a promising source for the regeneration of periodontal tissues. However, these cells may experience functional alterations during in vitro expansion and culture, as well as under inflammatory conditions. In this study, we investigated AS2863619 (AS), a small molecule drug with potential applications in immune disease research, to evaluate its effects on the osteogenic potential of PDLSCs and to elucidate the underlying mechanisms of action.
Methods The effect of AS on the proliferation of PDLSCs was evaluated using a CCK-8 assay. The osteogenic potential of PDLSCs was assessed through alkaline phosphatase (ALP) activity staining or Alizarin Red S (ARS) staining to detect mineralized nodules. Inflammatory injury in PDLSCs was induced by treating them with recombinant human tumor necrosis factor-alpha (rhTNF-α) . Transcriptome sequencing was employed to investigate the potential target of AS for rescuing osteogenic differentiation from inflammatory injury. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blot analysis were conducted to determine the expression levels of osteogenic gene markers and associated signaling pathways. Various small molecule inhibitors, agonists, and recombinant proteins were utilized to modulate relevant signaling pathways as specified in the text.
Results The proliferation of PDLSCs was inhibited by AS at 250 nM after 1 day of culture, whereas AS at 125 nM inhibited their proliferation after 3 and 5 days of culture. Additionally, AS significantly enhanced the ALP activity of PDLSCs and accelerated the formation time of mineralized nodules, while suppressing their adipogenic differentiation ability. Furthermore, AS promoted the levels of bone morphogenetic protein (BMP)2 and phosphorylated (p)-SMAD1/5 without significant effects on p-ERK, p-P38, and p-JNK levels. Moreover, AS effectively augmented rhTNF-α-inhibited ALP activity in PDLSCs and attenuated rhTNF-α-induced components involved in retinoic acid-inducible gene I (RIG-I)-like receptor signaling pathway including RIG-I, MDA5, and ISG15.
Conclusions AS is a potent small molecule that effectively enhances the osteogenic potential of PDLSCs, potentially by augmenting BMP2/SMAD signaling. Additionally, AS significantly improves the inflammation-induced impairment of osteogenic potential in PDLSCs and may contribute to osteogenic rescue through inhibition of rhTNF-α-induced RIG-I-like receptor signaling pathway.
Reef Foraminifera: Artificial vs. Natural Environments
Authors: MINHAT FATIN IZZATI , Shaari Hasrizal , Saelan Wan Nurzalia Wan , Abdullah Farah Amelia Bee , Malek Muhammad Naim Abdul , Yang Shouye , Sharudin Nursyafiqah Syazana 
Published: November 2024

Abstract

The success of artificial reefs is influenced by localised oceanographic factors including wave exposure, sedimentation rates, and proximity to natural reef environments. The Bidong Shipwreck, on the shallow shelf of the South China Sea, is a hotspot for meiobenthos. This study compared foraminifera distribution at the Bidong Shipwreck with that of the adjacent natural reef of Bidong Island. Foraminiferal assemblages at the shipwreck were less diverse. Amphistegina spp. were the most dominant genus at both study sites. Calcarinids—such as Calcarina and Neorotalia spp.—occurred only on the natural reef. Cluster analysis categorised the foraminiferal assemblages from both sites into three distinct groups based on substrate preference. Group A consisted of foraminiferal assemblages from the shallow reef area of Bidong Island with a sandy substrate, where Nummulites and Peneroplis spp. were indicator species. Group B represented a mix of stations from both the shipwreck and natural reef, characterised by water depths > 18 m and a muddy sand substrate, with Amphistegina spp. comprising ~ 80% of this assemblage. Group C displayed a more diverse distribution of foraminiferal species, including a mix of symbiont-bearing and heterotrophic taxa coexisting within the coarser sediments. Despite being over 500 years old, the Bidong Shipwreck does not exhibit a foraminiferal assemblage similar to that of the nearby natural reef. Differences in substrate type and water depth between these sites caused foraminiferal assemblage shifts. This suggests that although artificial reefs can have beneficial effects on fish biomass and meiobenthic communities, their impact on protists such as foraminifera can be markedly different.
Association of Gallstone Disease with Increased Risk of Sarcopenia: A Cross-Sectional Study
Authors: Zeng Di , Lu Jiong , Chen Jinhong 
Published: November 2024

Abstract

Background: Musculoskeletal disorders related to chronic liver and biliary diseases are increasingly recognized, with a growing body of observational studies linking these conditions. This study investigates the association between gallstone disease and sarcopenia, exploring the implications for patient health outcomes.
Methods: A cross-sectional analysis was conducted involving 1,683 participants to assess the relationship between gallstones and sarcopenia. Adjusted odds ratios (OR) for sarcopenia were calculated using logistic regression models, controlling for sociodemographic and health-related factors.
Results: Among the participants, those with gallstones had a higher likelihood of sarcopenia, with adjusted odds ratios of 2.15 in Model 1 and 2.71 in Model 2. The risk was particularly pronounced in females, with an odds ratio of 2.756. Additionally, individuals with diabetes faced an elevated odds ratio of 8.576 for developing sarcopenia. Although trends in increased risk were observed in other subgroups, such as those with hypertension and younger adults, these did not achieve statistical significance. An inverse relationship was also noted between appendicular skeletal muscle mass (ASM) and gallstone occurrence.
Conclusion: This study demonstrates that gallstone disease increases the risk of sarcopenia, highlighting the need for integrated management strategies that include nutritional evaluations and exercise interventions. Further longitudinal research is essential to clarify causal relationships and underlying mechanisms.
The association between Healthy Lifestyle Score and odds of Non-alcoholic Fatty Liver Disease (NAFLD)
Authors: Hedayati Safoora , Doostmohammadian Azam , Jambarsang Sara , Maadi Mansooreh , Zamani Farhad , Hoseinzadeh Mahdieh 
Published: November 2024

Abstract

Background: This study aimed to determine the association between the Healthy Lifestyle Score (HLS), as a comprehensive index that reflects the combined role of four lifestyle environmental factors, including weight, physical activity, diet, and smoking, with the odds of Non-Alcoholic Fatty Liver Disease (NAFLD).
Methods: This cross-sectional study was conducted on 2979 participants aged 18–70 years in AmolCS framework. Dietary intake information was determined using a valid food frequency questionnaire. The diet quality was measured using the Alternative Healthy Eating Index (AHEI). The Healthy Lifestyle Score (HLS) was calculated based on four dichotomous components including smoking status, BMI kg/m2, physical activity and AHEI score. To detect NAFLD in participants an ultrasound scan of the liver was used. Multiple logistic regression models were used to determine the odds ratios of NAFLD across tertiles of HLS.
Results: In the full adjust model, after adjusting for age, sex, physical activity, residency, and energy intake the odds of NAFLD were decreased among the HLS tertiles (OR:0.71; 95%, CI: 0.55-0.91). There was a significant decreasing trend in the odds of NAFLD across increasing tertiles of HLS(p-trend: <0.001). However, AHEI did not differ between healthy people and NAFLD patients (p >0.05).
Conclusions: Our study showed that a diet with a high AHEI score as an independent factor cannot decrease the odds of NAFLD, but adherence to a healthy diet, alongside other components of the HLS including normal BMI, not smoking, and high physical activity are associated with reduced odds of NAFLD by 62 percent.
Delayed chemotherapy-induced nausea – Occurrence and corelates in an international multi-center study in adult oncology patients in clinical practice (CINrate)
Authors: Engst Ramona , Glaus Agnes , Mößner Ulrike , Ott Stefan , Koller Antje 
Published: November 2024

Abstract

Purpose Nausea and vomiting negatively impact patients' quality of life and may influence systemic antitumoral therapy (CHT). Delayed chemotherapy-induced nausea (dCIN), with low and minimally emetogenic CHT, is often underestimated in clinical practice. This study aims to assess dCIN occurrence, focusing on low and minimally emetogenic CHT.Methods In an international multicenter cross-sectional study, patients rated dCIN on a 0-100 scale for five days. The primary endpoint was dCIN in low and minimally emetogenic CHT. Secondary endpoints included known risk factors for CIN and vomiting, like diagnosis, gender etc. The relationship between dCIN and secondary endpoints was determined with a multiple logistic regression model.Results In three day-clinics of accredited cancer centers, 2 in Switzerland and 1 in Southern Germany, 172 patients were recruited. Of these, 31 (18%) received minimally, 65 (38%) low, 61 (36%) moderately and 15 (9%) highly emetogenic CHT. Occurrence of dCIN in low emetogenic CHT was 18.5% (n = 12 of n = 65; 95%CI 10.5–29.1), in minimally emetogenic CHT 3% (n = 1 of n = 31; 95%CI 0.04/14.1). Overall occurrence of dCIN was 18.0% (95%CI 12.8/24.3). Only 3 patients (1.7%; 95% CI 0.5/4.6) experienced vomiting. Stable risk factors for dCIN were diagnosis with a gastrointestinal tumor and younger age. Emetogenicity of antitumoral therapy, fear and nausea in former cycles did not remain in the model.Discussion In clinical settings, acute CIN and vomiting are generally well-managed, but dCIN still poses some challenges. Known risk factors and current antiemetic regimens offer only limited support for the prevention of the remaining occurrence of dCIN.
Investigating influencing factors of medication adherence to inhalers in COPD patients: A study based on the Anderson model
Authors: Yin Chengchen , Luo Yongyong , Shen Kaili , Mao Guangxin , Tang Fushan 
Published: November 2024

Abstract

Background
Chronic obstructive pulmonary disease (COPD) is a leading cause of mortality globally, imposing significant economic burden due to treatment costs. Non-adherence to inhaler therapy is common among COPD patients, contributing to increased hospitalization risk, disease progression, mortality rates, and reduced quality of life.
Objective
To investigate the factors influencing inhaler adherence in COPD patients and provide insights, for improving adherence.
Methods
A questionnaire based on Anderson model was administered to, COPD patients discharged from three tertiary hospitals in Zunyi City and those prescribed inhalers in respiratory departments.
Results
Only 30.90% of patients demonstrated good adherence to inhalers. Key influencing factors identified included contextual characteristics (e.g., management of chronic illness cards), and individual characteristics (e.g., family history, medication beliefs).
Conclusion
COPD patients exhibit suboptimal inhaler adherence. Pharmacists, in collaboration with healthcare professionals, communities, and patient families, should implement targeted interventions to address adherence-related factors and enhance patient quality of life.
Investigating the effect of breastfeeding counselling based on Latch, Position and Information-Motivation-Behavior Model on maternal and neonatal indices: A clinical trial
Authors: Apoorvari Masoume Abdollahi , Haji-Maghsoudi Saiedeh , Ahmadi Atefeh 
Published: November 2024

Abstract

objectives
This study compares the impact of breastfeeding counselling based on the Information-Motivation-Behavior model (IMB) with routine counselling at the breastfeeding counselling center on certain health indices of mothers and their infants.
Methods
The present study is a clinical trial using a convenient sampling method conducted randomly on the referred mothers and their infants. Participants were allocated into two groups "routine counseling at the breastfeeding counseling center" and "breastfeeding counseling based on the Information-Motivation-Behavior Model" (59 mothers and their infants in each group). Demographic information, WHO breastfeeding observation form, latch assessment form, infant weight, maternal body mass index (BMI), blood pressure, and postpartum depression questionnaire were filled. Outcomes were reassessed at 2, 4, and 6 months of children’s age for both groups. The infant behaviour questionnaire was completed only at 6 months.
Results
There was no significant difference in demographic variables between the two groups (p > 0.05). In both the routine and intervention groups, maternal blood pressure showed statistically significant changes over time (p < 0.05). In the intervention group, there were significant differences in postpartum depression scores (p < 0.05), maternal BMI (p < 0.05), infant weight scores (p < 0.05), latch assessment scores (p < 0.05), and total score of the WHO breastfeeding observation form (p < 0.05) compared to the routine counselling group. There was no significant statistical difference in infant behaviour scores between the routine and intervention groups (p > 0.05).
Conclusion
Application of counselling with the IMB model can motivate correct breastfeeding behaviours and improve maternal and infant health indices
Trial registration
This trial is registered with the Iranian Registry of Clinical Trial with the identifier IRCT20170611034452N15 (registration date 20230428)
Co-Led Dialectical Behavioral Therapy Skills and Cultural Teachings for Perinatal Persons who are Substance Involved
Authors: Gulbransen Kristen , Shingoose Geraldine , Ashdown Heather , Watson Heather , Phillips-Beck Wanda , Thiessen Kellie 
Published: November 2024

Abstract

Background
This study explored perinatal patient experiences in a co-led Dialectical Behavior Therapy (DBT) and Indigenous cultural teachings program. The aim was to understand the programs strengths, impact, and areas for improvement.
Methods
Guided by the Valuing All Voices Framework and using Appreciative Inquiry, interviews were conducted with participants (n = 9) and providers (n = 3) to capture their perspectives on participating and facilitating in the co-led session.
Results
The DBT and Indigenous cultural teachings, co-led by an Indigenous Knowledge Carrier and a Psychiatrist, were presented through a strengths-based approach. Participants engaged with the teachings across the four sessions, which fostered increased hope, emotional regulation, and resilience. They also developed better coping skills, recognized the importance of their self-identity, and strengthened their positive connection to their culture.
Conclusion
The study illustrates a promising DBT and cultural teaching approach for integrating culturally appropriate teachings within perinatal mental health care, responding to calls for Truth and Reconciliation, and addressing health disparities.
Large arterial ring formed by a duplicate origin of the middle cerebral artery (MCA) with a frontal branch arising from the superior channel of the ring (main MCA) diagnosed by magnetic resonance angiography
Authors: Uchino Akira , Kimura Hiroshi 
Published: November 2024

Abstract

Purpose
To describe a case of large arterial ring formed by a duplicate origin of the right middle cerebral artery (MCA) associated with a frontal branch arising from the superior channel of the ring (main MCA) using magnetic resonance (MR) angiography.
Methods
An 81-year-old man with spinocerebellar degeneration underwent cranial MR imaging and MR angiography using a 1.5-Tesla scanner. MR angiography was performed using a standard 3-dimensional time-of-flight technique.
Results
MR imaging showed cerebellar atrophy and multiple cerebral white matter lesions. Regarding family history, he was diagnosed with Machado-Joseph disease. On MR angiography, an anomalous artery arose from the lateral wall of the supraclinoid segment of the right internal carotid artery (ICA) and fused with the main MCA, forming a large arterial ring. Thus, the anomalous artery was diagnosed as a duplicate origin of the MCA and not a duplicated MCA. The frontal branch of the MCA arose from the superior channel of the arterial ring (main MCA).
Conclusion
Using catheter angiography, a case of a large arterial ring formed by a duplicate origin of the MCA associated with the frontal branch arising from the superior channel of the ring (main MCA) was previously reported. This variation is called “segmental duplication of the MCA.” However, this term may be confused with a duplicate origin or fenestration of the proximal M1 segment of the MCA. We report the first case of this combined variation diagnosed using MR angiography.
A phase II trial of anlotinib plus EGFR-TKIs in advanced non-small cell lung cancer with gradual, oligo, or potential progression after EGFR-TKIs treatment (CTONG-1803/ALTER-L001)
Authors: Chen Hua-Jun , Tu Hai-Yan , Hu Yanping , Fan Yun , Wu Guowu , Cang Shundong , Yang Yi , Yang Nong , Ma Rui , Jin Gaowa , Xu Ximing , Liu Anwen , Tang Shubin , Cheng Ying , Yu Yan , Xu Chong-Rui , Zhou Qing , Wu Yi-Long 
Published: November 2024

Abstract

Background: The study is to evaluate the efficacy and safety of combined anlotinib and EGFR-tyrosine kinase inhibitors (TKIs) in patients with advanced non-small cell lung cancer (NSCLC) who had gradual, oligo, or potential progression after previous EGFR-TKIs treatment.
Methods: We conducted an open-label, single-arm, multicenter, phase II trial in China. Eligible patients were 18-75 years old with histologically or cytologically confirmed NSCLC who were EGFR mutation positive and showed gradual, oligo, or potential progression after EGFR-TKIs. Anlotinib (12 mg/day) was administered orally for 2 weeks and then off 1 week in a 3-week cycle. EGFR-TKIs were continue used. The primary endpoint was progression-free survival (PFS). The secondary endpoints included 6- and 12-month PFS rate, objective response rate (ORR), disease control rate (DCR), overall survival (OS) and safety.
Results: From July 2019 to December 2022, 120 patients were enrolled. The median PFS (mPFS) was 9.1 months (95% CI 6.8-11.7). The PFS rates at 6 and 12 months was 68.5% and 38.8% respectively. For 86 patients with first-line 1st/2nd generation EGFR-TKIs, the mPFS was 9.2 months (95% CI 6.7-12.6). For 32 patients with first-line 3rd generation EGFR-TKIs, the mPFS was 10.3 months (95% CI 6.1-13.3). Overall ORR and DCR were 6.7% (95% CI 2.9-12.7) and 87.5% (95% CI 80.2-92.8), respectively. 52.5% of patients had grade 3 or higher treatment-emergent adverse events (TEAEs).
Conclusion: Anlotinib in combination with continuation of EGFR-TKIs led to prolonged clinical benefit of EGFR-TKIs, demonstrating favorable survival outcomes and manageable toxicity in NSCLC patients treated with EGFR-TKIs and had gradual, oligo, or potential progression.
Trial registration: NCT04007835
Urban Planning for Disaster Risk Reduction: A Systematic Review of Essential Requirements
Authors: Ferreira Jairo Filho Sousa de Almeida , Cortese Tatiana Tucunduva Philippi , Yigitcanlar Tan 
Published: November 2024

Abstract

Urban planning is critical in mitigating the impacts of disasters, enhancing community resilience and promoting sustainable development. This review study systematically analyzes the role of urban planning in disaster risk reduction (DRR) through a Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) approach. By reviewing scholarly articles and case studies, this paper examines various urban planning strategies that contribute to DRR, including land use planning, infrastructure development, risk mapping, and community engagement. The findings highlight the effectiveness of integrating risk assessments into urban planning processes, the importance of adaptive infrastructure design, and the need for inclusive planning practices that involve local communities in decision-making. The review also identifies challenges such as inadequate policy implementation, lack of resources, and the need for interdisciplinary collaboration, analyzing participation and academic importance, and correlating the publication of papers with the number of reported disasters. Through a comprehensive analysis of existing literature, this review underscores the potential of urban planning to reduce disaster risks and enhance urban resilience. The paper concludes with recommendations for policymakers, urban planners, and researchers to strengthen DRR initiatives via strategic urban planning practices. This review contributes to the growing body of knowledge in DRR and emphasizes the critical role of urban planning in creating safer, more resilient cities.
Suppression of SIGMAR1 hinders oral cancer cell growth via modulation of mitochondrial Ca2+ dynamics
Authors: Chagas Pablo Shimaoka , Garcia Cristiana Bernadelli , Chagas Henrique Izumi Shimaoka , Yeudall William Andrew , Yu Jack C , Baban Babak , Leopoldino Andréia Machado 
Published: November 2024

Abstract

Oral cancer is the most common malignancy of the oral cavity and facial region, affecting the mucosal and epithelial surfaces in the mouth and lips. Unfortunately, OC is often associated with a high mortality rate and limited treatment options for patients. Herein, we used in silico analysis and in vitro assays to investigate the impact of the Sigma-1 receptor (SIGMAR1) in OC progression by evaluating mitochondrial function, calcium signaling and clonogenic growth. First, the data from the TCGA pan-cancer analysis revealed that SIGMAR1 was overexpressed in OC versus healthy tissue and related to a worse survival rate. Furthermore, we also demonstrated that SIGMAR1 silencing increased mitochondrial membrane potential, inhibited Ca2+ influx and significantly decreased clonogenic growth of OC cells. Based on these findings, we suggest that SIGMAR1 may influence mitochondrial membrane potential and energy production by modulating Ca2+ uptake, which is critically important to cellular survival. In addition, SIGMAR1 knockdown may offer a potential strategy to be further explored as treatment for OC.
Assessment of inequity of access to intensive care units in Mexico City.
Authors: Real-Ramírez Janet , Hernández-Gilsoul Thierry , Pérez-Méndez Ayari , Sánchez-Hurtado Luis , Campos-Calderón Luis Fernando 
Published: November 2024

Abstract

OBJECTIVE
The objective was to describe the infrastructure and organization of ICU´s across the healthcare system of Mexico City.
METHODS
This is a descriptive cross-sectional study and geospatial analysis, on the number of ICU beds, in population with and without social security, and thematic maps of population density for 2020.
RESULTS
There were a total of 655 ICU beds distributed across 57 public hospitals, with a total of 0.87 ICU beds per capita. Inequity in accessibility was observed in the eastern and northern regions of Mexico City with 0.25–0.52 beds per capita for the SSSOP population.
CONCLUSIONS
We identified a limited ICU bed capacity per capita. This; combined with factors such as regional access and health care systems to which patients are entitled, increases the probability of death due to these health inequities. This supports the need for comprehensive and equitable planning of the infrastructure for health emergencies in Mexico City.