bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polychlorinated biphenyls reduce the kinematics contractile properties of embryonic stem cells-derived cardiomyocytes by disrupting their intracellular Ca2+ dynamics
Authors: Rebuzzini Paola , Zuccolo Estella , Civello Cinzia , Fassina Lorenzo , Arechaga Juan , Izquierdo Amaia , Faris Pawan , Zuccotti Maurizio , Moccia Francesco , Garagna Silvia 
Published: December 2018

AbstractPersistent organic pollutants are a group of chemicals that include polychlorinated biphenyls (PCBs). PCBs exposure during adult life increases incidence and severity of cardiomyopathies, whereas in utero exposure determines congenital heart defects. Being fat-soluble, PCBs are passed to newborns through maternal milk, impairing heart functionality in the adult. It is still unknown how PCBs impair cardiac contraction at cellular/molecular levels. Here, we study the molecular mechanisms by which PCBs cause the observed heart contraction defects, analysing the alterations of Ca2+ toolkit components that regulate contraction. We investigated the effect that Aroclor 1254 (Aroclor), a mixture of PCBs, has on perinatal-like cardiomyocytes derived from mouse embryonic stem cells. Cardiomyocytes, exposed to 1 or 2 µg/ml Aroclor for 24 h, were analyzed for their kinematics contractile properties and intracellular Ca2+ dynamics. We observed that Aroclor impairs cardiomyocytes contractile properties by inhibiting spontaneous Ca2+ oscillations. It disrupts intracellular Ca2+ homeostasis by reducing the sarcoplasmic reticulum Ca2+ content and by inhibiting voltage-gated Ca2+ entry. These findings contribute to the understanding of the molecular underpinnings of PCBs-induced cardiovascular alterations, which are emerging as an additional life-threatening hurdle associated to PCBs pollution. Therefore, PCBs-dependent alteration of intracellular Ca2+ dynamics is the most likely trigger of developmental cardiac functional alteration.
Extracellular vesicles are associated with the systemic inflammation of patients with seropositive rheumatoid arthritis
Authors: Burbano Catalina , Rojas Mauricio , Muñoz-Vahos Carlos , Vanegas-García Adriana , Correa Luis A. , Vásquez Gloria , Castaño Diana 
Published: December 2018

AbstractPatients with rheumatoid arthritis (RA) and autoantibodies, such as rheumatoid factor and those against cyclic citrullinated peptides, are designated as seropositive and have a more severe disease with worse prognosis than seronegative RA patients. Understanding the factors that participate in systemic inflammation, in addition to articular commitment, would allow better treatment approaches for prevention of RA comorbidities and disease reactivation. We evaluated whether monocyte subsets and extracellular vesicles (EVs) could contribute to this phenomenon. Seropositive patients had higher levels of proinflammatory cytokines than those of seronegative patients and healthy controls (HCs); however, this systemic inflammatory profile was unrelated to disease activity. High frequencies of circulating EVs positive for IgG, IgM, CD41a, and citrulline, together with altered counts and receptor expression of intermediate monocytes, were associated with systemic inflammation in seropositive patients; these alterations were not observed in seronegative patients, which seem to be more similar to HCs. Additionally, the EVs from seropositive patients were able to activate mononuclear phagocytesin vitro, and induced proinflammatory cytokines that were comparable to the inflammatory response observed at the systemic level in seropositive RA patients; therefore, all of these factors may contribute to the greater disease severity that has been described in these patients.
Time-resolved synchrotron X-ray micro-tomography datasets of drainage and imbibition in carbonate rocks
Authors: Singh Kamaljit , Menke Hannah , Andrew Matthew , Rau Christoph , Bijeljic Branko , Blunt Martin J. 
Published: December 2018

AbstractMultiphase flow in permeable media is a complex pore-scale phenomenon, which is important in many natural and industrial processes. To understand the pore-scale dynamics of multiphase flow, we acquired time-series synchrotron X-ray micro-tomographic data at a voxel-resolution of 3.28 μm and time-resolution of 38 s during drainage and imbibition in a carbonate rock, under a capillary-dominated flow regime at elevated pressure. The time-series data library contains 496 tomographic images (gray-scale and segmented) for the complete drainage process, and 416 tomographic images (gray-scale and segmented) for the complete imbibition process. These datasets have been uploaded on the publicly accessible British Geological Survey repository, with the objective that the time-series information can be used by other groups to validate pore-scale displacement models such as direct simulations, pore-network and neural network models, as well as to investigate flow mechanisms related to the displacement and trapping of the non-wetting phase in the pore space. These datasets can also be used for improving segmentation algorithms for tomographic data with limited projections.
Gene-gene and gene-environment interaction data for platinum-based chemotherapy in non-small cell lung cancer
Authors: Wang Lei-Yun , Cui Jia-Jia , Liu Jun-Yan , Guo Ao-Xiang , Zhao Zhen-Yu , Liu Ying-Zi , Wu Ji-Chu , Li Min , Hu Cheng-Ping , Gao Yang , Zhou Hong-Hao , Yin Ji-Ye 
Published: December 2018

AbstractGene-gene (GXG) and gene-environment (GXE) interactions play important roles in pharmacogenetics study. Simultaneously incorporating multiple single nucleotide polymorphisms (SNPs) and clinical factors is needed to explore the association of their interactions with drug response and toxicity phenotypes. We genotyped 504 SNPs in a total of 490 Chinese non-small cell lung cancer (NSCLC) patients, and the correlation of GXG and GXE interactions with platinum-based chemotherapeutic efficacy and safety were analyzed. In this data descriptor, we shared our data set which could help others to reuse them. All kinds of file types needed for GXG and GXE analysis were supplied. The process of genotyping and data analysis was also introduced step by step.
Functional and structural studies of tolloid-like 1 mutants associated with atrial-septal defect 6
Authors: Sieron Lukasz , Lesiak Marta , Schisler Izabela , Drzazga Zofia , Fertala Andrzej , Sieron Aleksander L. 
Published: December 2018

Abstract
Inactive mammalian tolloid-like 1 (tll1) and mutations detected in tolloid-like 1 (TLL1) have been linked to the lack of the heart septa formation in mice and to a similar human inborn condition called atrial-septal defect 6 (ASD6; OMIM 613087, formerly ASD II). Previously, we reported four point mutations in TLL1 found in approximately 20% of ASD6 patients. Three mutations in the coding sequence were M182L, V238A, and I629V. In this work, we present the effects of these mutations on TLL1 function. Three recombinant cDNA constructs carrying the mutations and one wild-type construct were prepared and then expressed in HT-1080 cells. Corresponding recombinant proteins were analyzed for their metalloendopeptidase activity using a native substrate, chordin. The results of these assays demonstrated that in comparison with the native TLL1, mutants cleaved chordin and procollagen I at significantly lower rates. CD analyses revealed significant structural differences between the higher order structure of wild-type and mutant variants. Moreover, biosensor-based assays of binding interactions between TLL1 variants and chordin demonstrated a significant decrease in the binding affinities of the mutated variants. The results from this work indicate that mutations detected in TLL1 of ASD6 patients altered its metalloendopeptidase activity, structure, and substrate-binding properties, thereby suggesting a possible pathomechanism of ASD6.
Rosetta/OSIRIS observations of the 67P nucleus during the April 2016 flyby: high-resolution spectrophotometry
Authors: Feller C. , Fornasier S. , Ferrari S. , Hasselmann P. H. , Barucci A. , Massironi M. , Deshapriya J. D. P. , Sierks H. , Naletto G. , Lamy P. L. , Rodrigo R. , Koschny D. , Davidsson B. J. R. , Bertaux J.-L. , Bertini I. , Bodewits D. , Cremonese G. , Da Deppo V. , Debei S. , De Cecco M. , Fulle M. , Gutiérrez P. J. , Güttler C. , Ip W.-H. , Keller H. U. , Lara L. M. , Lazzarin M. , López-Moreno J. J. , Marzari F. , Shi X. , Tubiana C. , Gaskell B. , La Forgia F. , Lucchetti A. , Mottola S. , Pajola M. , Preusker F. , Scholten F. 
Published: December 2018

Context.From August 2014 to September 2016, the Rosetta spacecraft followed comet 67P/Churyumov–Gerasimenko along its orbit. After the comet passed perihelion, Rosetta performed a flyby manoeuvre over the Imhotep–Khepry transition in April 2016. The OSIRIS/Narrow-Angle-Camera (NAC) acquired 112 observations with mainly three broadband filters (centered at 480, 649, and 743 nm) at a resolution of up to 0.53 m/px and for phase angles between 0.095° and 62°.Aims.We have investigated the morphological and spectrophotometrical properties of this area using the OSIRIS/NAC high-resolution observations.Methods.We assembled the observations into coregistered color cubes. Using a 3D shape model, we produced the illumination conditions and georeference for each observation. We mapped the observations of the transition to investigate its geomorphology. Observations were photometrically corrected using the Lommel–Seeliger disk law. Spectrophotometric analyses were performed on the coregistered color cubes. These data were used to estimate the local phase reddening.Results.The Imhotep–Khepry transition hosts numerous and varied types of terrains and features. We observe an association between a feature’s nature, its reflectance, and its spectral slopes. Fine material deposits exhibit an average reflectance and spectral slope, while terrains with diamictons, consolidated material, degraded outcrops, or features such as somber boulders present a lower-than-average reflectance and higher-than-average spectral slope. Bright surfaces present here a spectral behavior consistent with terrains enriched in water-ice. We find a phase-reddening slope of 0.064 ± 0.001%/100 nm/° at 2.7 au outbound, similar to the one obtained at 2.3 au inbound during the February 2015 flyby.Conclusions.Identified as the source region of multiple jets and a host of water-ice material, the Imhotep–Khepry transition appeared in April 2016, close to the frost line, to further harbor several potential locations with exposed water-ice material among its numerous different morphological terrain units.
Systematic investigation of chemical abundances derived using IR spectra obtained with GIANO
Authors: Caffau E. , Bonifacio P. , Oliva E. , Korotin S. , Capitanio L. , Andrievsky S. , Collet R. , Sbordone L. , Duffau S. , Sanna N. , Tozzi A. , Origlia L. , Ryde N. , Ludwig H.-G. 
Published: December 2018

Context. Detailed chemical abundances of Galactic stars are needed in order to improve our knowledge of the formation and evolution of our galaxy, the Milky Way.
Aims. We took advantage of the GIANO archive spectra to select a sample of Galactic disc stars in order to derive their chemical inventory and to compare the abundances we derived from these infrared spectra to the chemical pattern derived from optical spectra.
Methods. We analysed high-quality spectra of 40 stars observed with GIANO. We derived the stellar parameters from the photometry and the Gaia data-release 2 (DR2) parallax; the chemical abundances were derived with the code MyGIsFOS. For a subsample of stars we compared the chemical pattern derived from the GIANO spectra with the abundances derived from optical spectra. We derived P abundances for all 40 stars, increasing the number of Galactic stars for which phosphorus abundance is known.
Results. We could derive abundances of 14 elements, 8 of which are also derived from optical spectra. The comparison of the abundances derived from infrared and optical spectra is very good. The chemical pattern of these stars is the one expected for Galactic disc stars and is in agreement with the results from the literature.
Conclusions. GIANO is providing the astronomical community with an extremely useful instrument, able to produce spectra with high resolution and a wide wavelength range in the infrared.
Diagnosis of A3 Pulley Injuries Using Ultrasound
Authors: Schöffl Isabelle , Deeg Johannes , Lutter Christoph , Bayer Thomas , Schöffl Volker 
Published: December 2018

Abstract
Rationale and Objectives Pulley ruptures are the most common injuries in sport climbing. Ruptures of the A2 and A4 pulleys have been studied extensively, and ultrasound has proven to be a highly sensitive and specific tool for their diagnosis. However, the correct diagnosis of A3 pulley ruptures continues to be a challenge. Therefore, we investigated a novel approach to this pathology.
Material and Methods Eighteen fingers from nine different human cadavers were examined using high-resolution, dynamic ultrasound before and after being subjected to different combinations of singular and multiple iatrogenic pulley ruptures in a standardised fashion. Special attention was paid to the behaviour of the volar plate (VP) with respect to the proximal interphalangeal joint (PIP) and the flexor tendons before and after pulley rupture.
Results Injuries to the A2 and A4 pulleys were diagnosed via ultrasound with sensitivities of 90 % and 94 % and specificities of 100 % and 97 %, respectively. A direct visualisation of the A3 pulley was achieved in 61 % of the fingers. The VP became significantly thicker and shorter during finger flexion as well as after A3 pulley rupture. The distance between tendon and VP became significantly more pronounced after A3 pulley rupture. For distances greater than 0.9 mm between VP and tendon, a sensitivity of 76 % and a specificity of 94 % were achieved for determining A3 pulley ruptures.
Conclusion The distance measurement between VP and tendon was found to be a valid indirect method for the diagnosis of A3 pulley ruptures. This approach is the first ultrasound method for accurately diagnosing A3 pulley ruptures.
Optimal timing for precutting in cases with difficult biliary cannulation
Authors: Takano Yuichi , Nagahama Masatsugu , Niiya Fumitaka , Kobayashi Takahiro , Yamamura Eiichi , Maruoka Naotaka 
Published: December 2018

Abstract
Background and study aims In endoscopic retrograde cholangiopancreatography (ERCP), precutting is widely used when achieving biliary cannulation is difficult. However, no consensus has been reached with regard to the best time to initiate precutting.
Patients and methods We retrospectively examined 63 patients who underwent precutting for naïve papilla with difficulty in biliary cannulation between 2009 and 2016. The outcomes of the early precut group (≤ 20 min from cannulation until initiating precutting) and the late precut group (> 20 min) were compared.
Results Of the 63 patients, 17 (27 %) were in the early precut group and 46 (73 %) were in the late precut group; median time until the initiating precutting was 28 minutes (7 – 50). No significant difference was observed between the two groups in terms of clinical features (age, sex, and indication for ERCP), precutting method, and rate of pancreatic duct stent placement. Significantly higher rates of successful biliary cannulation were observed in the early precut group (16/17; 94 %) than in the late precut group (32/46; 70 %) (P < 0.05). In 13 patients in whom precutting was commenced after 40 minutes, the rate of successful biliary cannulation was very low at 53 % (7/13). No significant difference was found between the two groups in terms of incidence of complications (pancreatitis in 5 patients and bleeding in 1 patient).
Conclusion In actual clinical practice, precutting is commenced approximately 30 minutes after cannulation; however, to successfully achieve biliary cannulation, precutting is recommended to be performed within 20 minutes. Precutting is effective when little inflammation and swelling of the ampulla of Vater is observed. This study was limited in that it was single-center, retrospective and had a small subject sample.
Begrenzung der individuellen Plastizität
erkennen
Authors: Jacobs Gabi 
Published: December 2018

Zusammenfassung
Sackgasse? Das Bobath-Konzept basiert auf dem Wissen über die
Lernfähigkeit bzw. über die Plastizität des Gehirns. Wie funktioniert Lernen,
welche Lernwege sind sinnvoll? Wie kann beurteilt werden, ob das Gelernte zum
Vor- oder Nachteil wird? Kann Lernen auch in eine Sackgasse führen?
Objective image analysis of non-magnifying image-enhanced endoscopy for diagnosis of small depressed early gastric cancers
Authors: Watari Jiro , Kobayashi Masaya , Nakai Keisuke , Ito Chiyomi , Tamura Akio , Ogawa Tomohiro , Yamasaki Takahisa , Okugawa Takuya , Kondo Takashi , Kono Tomoaki , Tozawa Katsuyuki , Tomita Toshihiko , Oshima Tadayuki , Fukui Hirokazu , Morimoto Takeshi , Miwa Hiroto 
Published: December 2018

Abstract
Background Gastric cancers (GC) after H. pylori eradication are difficult to diagnose even by magnifying narrow-band imaging (NBI) or blue laser imaging (BLI) endoscopy. Little is known with regard to non-magnifying (NM)-NBI/BLI for early GC so we examined the efficacy of NM-NBI/BLI for early GC diagnosis.
Methods We retrospectively analyzed the images of 29 small (≤ 1 cm) intramucosal GC that had been treated with endoscopic submucosal dissection and 137 benign depressed lesions (BDLs). The brightness and shape of the GCs and BDLs by NM-NBI/BLI were assessed with ImageJ software.
Results The NBI/BLI-index, which indicates the brightness of NBI/BLI for visualization, was significantly higher in GC than BDLs in both the H. pylori-infected (P = 0.009) and -eradicated group (P < 0.0001), indicating that GC exhibited brighter colors than the normal surrounding mucosa. The C-index, which refers to the circularity of the lesion, was also significantly higher in GC than BDLs in both H. pylori-infected (P = 0.006) and -eradicated cases (P = 0.004). Based on receiver-operating characteristic curve analysis, cutoff values for the NBI/BLI- and C-indices for GC were 1.04 and 0.58 in the H. pylori-infected cases, and 0.98 and 0.64 in the H. pylori-eradicated cases. With the reference value of the NBI/BLI-index set at ≥ 0.69 with the C-index at ≥ 0.21 in the H. pylori-infected and the NBI/BLI-index at ≥ 0.80 with the C-index at ≥ 0.32 in the H. pylori-eradicated cases, both the sensitivity and negative predictive value for early GC were 100 %. A high NBI/BLI-index tended to be associated with a wide length of the intervening part histologically in the H. pylori-eradicated cases (P = 0.09).
Conclusions The small depressed-type early GC had brighter color and rounder shape compared to BDLs in both H. pylori-infected and -eradicated cases. The NBI/BLI- and C-indices calculated by the image analysis may facilitate identification of small depressed-type GC.
Clinical evaluation of the M-ANNHEIM classification: Development of the M-ANNHEIM-Surgery-Score as a new tool to monitor patients with chronic pancreatitis
Authors: Hirth Michael , Weiss Christel , Rückert Felix , Pfützer Roland , Wilhelm Torsten , Hetjens Svetlana , Hardt Philip , Gubergrits Natalia , Ebert Matthias , Schneider Alexander 
Published: December 2018

Abstract
Objectives The M-ANNHEIM classification of chronic pancreatitis (CP) stratifies degrees of disease severity according to the M-ANNHEIM-Severity-Score. We aimed to demonstrate the clinical usefulness of the M-ANNHEIM-Severity-Score in quantifying and predicting the frequency of pancreatic surgery, and to establish the M-ANNHEIM-Surgery-Score as a simplified system for patient surveillance regarding the demand of pancreatic surgery.
Methods We performed a retrospective, cross-sectional study with 741 CP patients (Mannheim/Germany, n = 537; Gießen/Germany, n = 100; Donetsk/Ukraine, n = 104) categorized according to the M-ANNHEIM classification.
Results We observed a significantly higher M-ANNHEIM-Severity-Score in patients that were classified within 7 days preceding pancreatic surgery than in individuals that did not require surgery (p < 0.001, Mann–Whitney-U-test). Using a logistic regression analysis with all variables of the M-ANNHEIM-Severity-Score, we established the M-ANNHEIM-Surgery-Score as a simplified new tool to identify patients that may require surgery. A receiver operating characteristic-analysis revealed a cut-off-value of 9 points within the M-ANNHEIM-Surgery-Score to identify these individuals (sensitivity 78.7 %, specificity 91 %). Based on the M-ANNHEIM-Surgery-Score, we defined three categories for demand of surgery with frequencies of pancreatic operations of 1.6 % (n = 7/440) in the “Baseline-Demand”-category, 7 % (n = 12/172) in the “Low-Demand”-category (p < 0.0001, Chi-square-test, OR 4.6, Confidence Interval (CI) 1.8 – 12), and 54 % (n = 70/129) in the “High-Demand”-category (p < 0.0001, OR 73, CI 32 – 167). Patients that were categorized for the “High-Demand”-category, but were not operated on, had a significantly increased ratio of clinical features that hamper performance of surgery (p < 0.001, Chi-square-test).
Conclusions The M-ANNHEIM-Surgery-Score represents a useful tool to monitor patients with CP and to estimate the demand of surgery in CP.
Advances in Delayed-Onset Muscle Soreness (DOMS): Part I: Pathogenesis and Diagnostics
Authors: Hotfiel Thilo , Freiwald Jürgen , Hoppe Matthias , Lutter Christoph , Forst Raimund , Grim Casper , Bloch Wilhelm , Hüttel Moritz , Heiss Rafael 
Published: December 2018

AbstractDelayed-onset muscle soreness (DOMS) is a type of ultrastructural muscle injury. The manifestation of DOMS is caused by eccentric or unfamiliar forms of exercise. Clinical signs include reduced force capacities, increased painful restriction of movement, stiffness, swelling, and dysfunction of adjacent joints. Although DOMS is considered a mild type of injury, it is one of the most common reasons for compromised sportive performance. In the past few decades, many hypotheses have been developed to explain the aetiology of DOMS. Although the exact pathophysiological pathway remains unknown, the primary mechanism is currently considered to be the ultrastructural damage of muscle cells due to unfamiliar sporting activities or eccentric exercise, which leads to further protein degradation, apoptosis and local inflammatory response. The development of clinical symptoms is typically delayed (peak soreness at 48 – 72 h post-exercise) as a result of complex sequences of local and systemic physiological responses. The following narrative review was conducted to present an overview of the current findings regarding the damaging mechanisms as well as the pathophysiology of DOMS and its diagnostic evaluation.
Novel three-dimensional imaging system may facilitate gastric endoscopic submucosal dissection procedure: an ex vivo animal study
Authors: Akizue Naoki , Matsumura Tomoaki , Maruoka Daisuke , Ishikawa Kentaro , Hang Dao , Okimoto Kenichiro , Saito Keiko , Nakagawa Tomoo , Arai Makoto , Kato Naoya 
Published: December 2018

Abstract
Background and study aims Endoscopic submucosal dissection (ESD) requires advanced skills to perform safely without complications. The current study evaluated the usefulness of a novel three-dimensional (3D) imaging system in ESD using porcine stomachs.
Methods Four endoscopists (two trainees and two experts) performed eight ESD procedures using both 3D and 2D images. The usefulness of 3D image versus 2D image was evaluated by visibility and procedure time. In addition, occurrence of eyestrain and dizziness in 3D image was assessed.
Results En bloc resection was successfully achieved, without perforation, in all cases. The evaluation score in the 3D image group was better than that in the 2D image group, particularly depth perception was statistically significantly good. No significant difference was found in the working speed between the 2D and 3D image groups. Two examiners experienced eyestrain and dizziness while using the 3D image.
Conclusions All the ESD procedures were performed safely. Depth perception using the 3D image was better than with the 2D image. A novel 3D image system may facilitate ESD.