bluuebunny/crossref_metadata_2025_split huggingface dataset:
New Radiocarbon Dates Show Early Neolithic Date of Flint-Mining and Stone Quarrying in Britain
Authors: Edinborough Kevan , Shennan Stephen , Teather Anne , Baczkowski Jon , Bevan Andrew , Bradley Richard , Cook Gordon , Kerig Tim , Parker Pearson Mike , Pope Alexander , Schauer Peter 
Published: August 2019

ABSTRACTNew radiocarbon (14C) dates suggest a simultaneous appearance of two technologically and geographically distinct axe production practices in Neolithic Britain; igneous open-air quarries in Great Langdale, Cumbria, and from flint mines in southern England at ~4000–3700 cal BC. In light of the recent evidence that farming was introduced at this time by large-scale immigration from northwest Europe, and that expansion within Britain was extremely rapid, we argue that this synchronicity supports this speed of colonization and reflects a knowledge of complex extraction processes and associated exchange networks already possessed by the immigrant groups; long-range connections developed as colonization rapidly expanded. Although we can model the start of these new extraction activities, it remains difficult to estimate how long significant production activity lasted at these key sites given the nature of the record from which samples could be obtained.
Negotiating Respectability: Comparing the experiences of poor and middle-class young urban women in India
Authors: TWAMLEY KATHERINE , SIDHARTH JUHI 
Published: August 2019

AbstractThis article draws together two studies: one that explores the intimate relationships of young slum-dwelling Dalit women in Mumbai and the other of young middle-class women in Baroda, Gujarat. Using an intersectional lens, we trace the ways that gendered ideals of respectability shape women's freedom of movement and relationships. The comparison produces new insights into the ways that class, caste, and location cut across gender to shape young women's lives in India. We argue that the distinctive positionings of the women structure the ways in which they react to gender norms and the means with which they strategize around them. Middle-class young women strike a ‘passive bargain’, upholding ideals of respectability by shoring up symbolic capital for a ‘good’ marriage and class privilege. The Dalit women show more active resistance to an ideal that they struggle to achieve, despite heavy control and surveillance over their movement and relationships. However, contrary to previous research, we show that both groups are beholden and lay claim to similar gendered and intimate ideals.
Use of Medication at the Roskilde (Denmark) Music Festival 2015 – A Prospective Observational Study of 15,133 Treated Attendees
Authors: Stagelund Søren , Andersen Kenneth Geving , Søe Jesper , Roslind Michelle , Wildgaard Kim 
Published: August 2019

AbstractBackground:Outdoor music festivals (OMFs) attract large numbers of guests and benefit from the help of large numbers of volunteers. Studies have previously described the injury patterns at OMFs, but no studies have described the use of medication and sales from on-site pharmacies at a large OMF.Method:The usage of medication and prescriptions in the Medical Health Care Organization (MHCO), including sales from the on-site pharmacy, at the Roskilde (Denmark) Festival 2015 were prospectively recorded.Results:In excess of 130,000 attendees (guests and volunteers) participated in the Roskilde Festival 2015. The number of attendees contacting the MHCO was 15,133, of which 3,723 (25%) had a consultation with a doctor. Of all attendees evaluated by a doctor, 669 attendees received some form of medication in relation to the consultation. The MHCO administered and/or handed out a total of 6,494 units of prescription and over-the-counter medication, of which analgesics represented nearly 51%. Asthma was the condition with the highest proportion of attendees requiring pharmaceutical treatment, as 28 out of 48 (58%) received medication during the consultation. Sixty-five attendees received both medicine and a prescription. The MHCO handed out 562 prescriptions. In total, 609 prescriptions were redeemed at the on-site pharmacy. Antibiotics represented more than 78% of all redeemed prescriptions at the on-site pharmacy.Conclusion:The most utilized medications were analgesics and antibiotics. The data indicate a need for on-site prophylaxis using tetanus toxoid in combination with diphtheria toxoid vaccine and an on-site pharmacy. The content of the formulary at a mass-gathering event should be based on: evacuation time by ambulance/helicopter to hospitals with the level of competence needed; types of conditions to be treated on-site; level of competencies of festival medical staff; expected incidence and type of illness and injuries; and treatment of acute, life-threatening illnesses and or injuries.
Triage Performance of School Personnel Using the SALT System
Authors: Celik Daniel H. , Mencl Francis R. , Debacker Michel , Kurland Lisa , Wilber Scott T. , Frey Jennifer A. 
Published: August 2019

AbstractIntroduction:The aim of this study was to determine if school personnel can understand and apply the Sort, Assess, Life-saving interventions, Treat/Transport (SALT) triage methods after a brief training. The investigators predicted that subjects can learn to triage with accuracy similar to that of medically trained personnel, and that subjects can pass an objective-structured clinical exam (OSCE) evaluating hemorrhage control.Methods:School personnel were eligible to participate in this prospective observational study. Investigators recorded subject demographic information and prior medical experience. Participants received a 30-minute lecture on SALT triage and a brief lecture and demonstration of hemorrhage control and tourniquet application. A test with brief descriptions of mass-casualty victims was administered immediately after training. Participants independently categorized the victims as dead, expectant, immediate, delayed, or minimal. They also completed an OSCE to evaluate hemorrhage control and tourniquet application using a mannequin arm.Results:Subjects from two schools completed the study. Fifty-nine were from a private school that enrolls early childhood through grade eight, and 45 from a public school that enrolls grades seven and eight (n = 104). The average subject age was 45 years and 68% were female. Approximately 81% were teachers and 87% had prior cardiopulmonary resuscitation (CPR) training. Overall triage accuracy was 79.2% (SD = 10.7%). Ninety-six (92.3%) of the subjects passed the hemorrhage control OSCE.Conclusions:After two brief lectures and a short demonstration, school personnel were able to triage descriptions of mass-casualty victims with an overall accuracy similar to medically trained personnel, and most were able to apply a tourniquet correctly. Opportunities for future study include integrating high-fidelity simulation and mock disasters, evaluating for knowledge retention, and exploring the study population’s baseline knowledge of medical care, among others.
US State constitutional entrenchment and default in the 19th century
Authors: Dove John A. , Young Andrew T. 
Published: August 2019

AbstractConstitutional scholars emphasize the importance of an enduring, stable constitutional order, which North and Weingast (1989) argue is consistent with credible commitments to sustainable fiscal policies. However, this view is controversial and has received little empirical study. We use 19th-century US state-level data to estimate relationships between constitutional design and the likelihood of a government default. Results indicate that more entrenched and less specific constitutions are associated with a lower likelihood of default.
Fate of weed seeds in spent mushroom compost following commercial mushroom production
Authors: Vollmer Kurt M. , VanGessel Mark J. 
Published: August 2019

AbstractCommercial mushroom producers grow several varieties of mushrooms on compost. Upon completion of the growing cycle, the spent mushroom compost is often sold as a soil amendment for both agricultural and homeowner use. Mushroom compost ingredients often come from fields infested with weeds, and in turn compost may spread unwanted weed seed. We conducted studies to assess the viability of weed seed following specific stages of the commercial mushroom production process. Weed seed was more likely to survive if the entire production process was not completed. However, no viable hairy vetch, Italian ryegrass, ivyleaf morningglory, Palmer amaranth, or velvetleaf remained at the end of the study. Although the seeds of most species were eliminated earlier in the composting process, ivyleaf morningglory required the complete process to eliminate 100% of the seed. These results indicate that spent mushroom compost is free of many weed species upon removal from mushroom houses and is unlikely to spread weed seed.
Thresholds for Atmospheric Convection in Amazonian Rainforests
Authors: Wu M. , Lee J.‐E. 
Published: August 2019

AbstractThe Amazon rainforest is known as the “Green Ocean” for its maritime‐like convection and cloud microphysics during the wet season. Although previous studies suggest the dominant thermodynamic processes involved in deep convection may differ between land and ocean, a comprehensive understanding of the thermodynamics of Amazonian convection is lacking. Using 404,971 daytime precipitating cloud profiles from the CloudSat satellite, we observe a regime transition from congestus dominance to cumulonimbus dominance when convective available potential energy exceeds a threshold in Amazonia and also in shrublands, but not in oceanic regions. In addition, the cloud regime transition is linked to boundary layer moisture in the two continental regions, while it is linked to lower‐free‐tropospheric moisture in the oceanic region. As the dry season progresses in Amazonia and modifies the free‐tropospheric stability, a moderate plant water stress and increased incoming solar energy facilitate the initiation of deep convection and the onset of the wet season.
Turbulence and Gas Transfer Velocities in Sheltered Flooded Forests of the Amazon Basin
Authors: MacIntyre Sally , Fernandes Amaral Joao H. , Barbosa Pedro M. , Cortés Alicia , Forsberg Bruce R. , Melack John M. 
Published: August 2019

AbstractSeasonally flooded forests along tropical rivers cover extensive areas, yet the processes driving air‐water exchanges of radiatively active gases are uncertain. To quantify the controls on gas transfer velocities, we combined measurements of water‐column temperature, meteorology in the forest and adjacent open water, turbulence with an acoustic Doppler velocimeter, gas concentrations, and fluxes with floating chambers. Under cooling, measured turbulence, quantified as the rate of dissipation of turbulent kinetic energy (ε), was similar to buoyancy flux computed from the surface energy budget, indicating convection dominated turbulence production. Under heating, turbulence was suppressed unless winds in the adjacent open water exceeded 1 m/s. Gas transfer velocities obtained from chamber measurements ranged from 1 to 5 cm/hr and were similar to or slightly less than predicted using a turbulence‐based surface renewal model computed with measured ε and ε predicted from wind and cooling.
Substorm‐Ring Current Coupling: A Comparison of Isolated and Compound Substorms
Authors: Sandhu J. K. , Rae I. J. , Freeman M. P. , Gkioulidou M. , Forsyth C. , Reeves G. D. , Murphy K. R. , Walach M.‐T. 
Published: August 2019

AbstractSubstorms are a highly variable process, which can occur as an isolated event or as part of a sequence of multiple substorms (compound substorms). In this study we identify how the low‐energy population of the ring current and subsequent energization varies for isolated substorms compared to the first substorm of a compound event. Using observations of H+ and O+ ions (1 eV to 50 keV) from the Helium Oxygen Proton Electron instrument onboard Van Allen Probe A, we determine the energy content of the ring current in L‐MLT space. We observe that the ring current energy content is significantly enhanced during compound substorms as compared to isolated substorms by ∼20–30%. Furthermore, we observe a significantly larger magnitude of energization (by ∼40–50%) following the onset of compound substorms relative to isolated substorms. Analysis suggests that the differences predominantly arise due to a sustained enhancement in dayside driving associated with compound substorms compared to isolated substorms. The strong solar wind driving prior to onset results in important differences in the time history of the magnetosphere, generating significantly different ring current conditions and responses to substorms. The observations reveal information about the substorm injected population and the transport of the plasma in the inner magnetosphere.
Physical and Biogeochemical Controls on pH Dynamics in the Northern Gulf of Mexico During Summer Hypoxia
Authors: Jiang Zong‐Pei , Cai Wei‐Jun , Chen Baoshan , Wang Kui , Han Chenhua , Roberts Brian J. , Hussain Najid , Li Qian 
Published: August 2019

AbstractHigh‐accuracy spectrophotometric pH measurements were taken during a summer cruise to study the pH dynamics and its controlling mechanisms in the northern Gulf of Mexico in hypoxia season. Using the recently available dissociation constants of the purified m‐cresol purple (Douglas & Byrne, 2017, https://doi.org/10.1016/j.marchem.2017.10.001; Müller & Rehder, 2018, https://doi.org/10.3389/fmars.2018.00177), spectrophotometrically measured pH showed excellent agreement with pH calculated from dissolved inorganic carbon (DIC) and total alkalinity over a wide salinity range of 0 to 36.9 (0.005 ± 0.016, n = 550). The coupled changes in DIC, oxygen, and nutrients suggest that biological production of organic matter in surface water and the subsequent aerobic respiration in subsurface was the dominant factor regulating pH variability in the nGOM in summer. The highest pH values were observed, together with the maximal biological uptake of DIC and nutrients, at intermediate salinities in the Mississippi and Atchafalaya plumes where light and nutrient conditions were favorable for phytoplankton growth. The lowest pH values (down to 7.59) were observed along with the highest concentrations of DIC and apparent oxygen utilization in hypoxic bottom waters. The nonconservative pH changes in both surface and bottom waters correlated well with the biologically induced changes in DIC, that is, per 100‐μmol/kg biological removal/addition of DIC resulted in 0.21 unit increase/decrease in pH. Coastal bottom water with lower pH buffering capacity is more susceptible to acidification from anthropogenic CO2 invasion but reduction in eutrophication may offset some of the increased susceptibility to acidification.
Ultra-sensitive digital quantification of proteins and mRNA in single cells
Authors: Lin Jing , Jordi Christian , Son Minjun , Van Phan Hoang , Drayman Nir , Abasiyanik Mustafa Fatih , Vistain Luke , Tu Hsiung-Lin , Tay Savaş 
Published: August 2019

AbstractSimultaneous measurement of proteins and mRNA in single cells enables quantitative understanding and modeling of cellular functions. Here, we present an automated microfluidic system for multi-parameter and ultra-sensitive protein/mRNA measurements in single cells. Our technology improves the sensitivity of digital proximity ligation assay by up to 55-fold, with a detection limit of 2277 proteins per cell and with detection efficiency of as few as 29 protein molecules. Our measurements using this system reveal higher mRNA/protein correlation in single mammalian cells than previous estimates. Furthermore, time-lapse imaging of herpes simplex virus 1 infected epithelial cells enabled by our device shows that expression of ICP4 -a major transcription factor regulating hundreds of viral genes- is only partially correlated with viral protein counts, suggesting that many cells go through abortive infection. These results highlight the importance of high-sensitivity protein/mRNA quantification for understanding fundamental molecular mechanisms in individual cells.
Anode interfacial layer formation via reductive ethyl detaching of organic iodide in lithium–oxygen batteries
Authors: Zhang Xiao-Ping , Sun Yi-Yang , Sun Zhuang , Yang Chu-Shu , Zhang Tao 
Published: August 2019

AbstractAs soluble catalysts, redox mediators can reduce the high charging overpotential of lithium-oxygen batteries by providing sufficient liquid-solid interface for lithium peroxide decomposition. However, the redox mediators usually introduce undesirable reactions. In particular, the so-called “shuttle effect” leads to the loss of both the redox mediators and electrical energy efficiency. In this study, an organic compound, triethylsulfonium iodide, is found to act bifunctionally as both a redox mediator and a solid electrolyte interphase-forming agent for lithium-oxygen batteries. During charging, the organic iodide exhibits comparable lithium peroxide-oxidizing capability with inorganic iodides. Meanwhile, it in situ generates an interfacial layer on lithium anode via reductive ethyl detaching and the subsequent oxidation. This layer prevents the lithium anode from reacting with the redox mediators and allows efficient lithium-ion transfer leading to dendrite-free lithium anode. Significantly improved cycling performance has been achieved by the bifunctional organic iodide redox mediator.
On-chip wavefront shaping with dielectric metasurface
Authors: Wang Zi , Li Tiantian , Soman Anishkumar , Mao Dun , Kananen Thomas , Gu Tingyi 
Published: August 2019

AbstractMetasurfaces can be programmed for a spatial transformation of the wavefront, thus allowing parallel optical signal processing on-chip within an ultracompact dimension. On-chip metasurfaces have been implemented with two-dimensional periodic structures, however, their inherent scattering loss limits their large-scale implementation. The scattering can be minimized in single layer high-contrast transmitarray (HCTA) metasurface. Here we demonstrate a one-dimensional HCTA based lens defined on a standard silicon-on-insulator substrate, with its high transmission (<1 dB loss) maintained over a 200 nm bandwidth. Three layers of the HCTAs are cascaded for demonstrating meta-system functionalities of Fourier transformation and differentiation. The meta-system design holds potential for realizing on-chip transformation optics, mathematical operations and spectrometers, with applications in areas of imaging, sensing and quantum information processing.
Identification of hyper-rewired genomic stress non-oncogene addiction genes across 15 cancer types
Authors: Hjaltelin Jessica Xin , Izarzugaza Jose M. G. , Jensen Lars Juhl , Russo Francesco , Westergaard David , Brunak Søren 
Published: August 2019

AbstractNon-oncogene addiction (NOA) genes are essential for supporting the stress-burdened phenotype of tumours and thus vital for their survival. Although NOA genes are acknowledged to be potential drug targets, there has been no large-scale attempt to identify and characterise them as a group across cancer types. Here we provide the first method for the identification of conditional NOA genes and their rewired neighbours using a systems approach. Using copy number data and expression profiles from The Cancer Genome Atlas (TCGA) we performed comparative analyses between high and low genomic stress tumours for 15 cancer types. We identified 101 condition-specific differential coexpression modules, mapped to a high-confidence human interactome, comprising 133 candidate NOA rewiring hub genes. We observe that most modules lose coexpression in the high-stress state and that activated stress modules and hubs take part in homoeostasis maintenance processes such as chromosome segregation, oxireductase activity, mitotic checkpoint (PLK1 signalling), DNA replication initiation and synaptic signalling. We furthermore show that candidate NOA rewiring hubs are unique for each cancer type, but that their respective rewired neighbour genes largely are shared across cancer types.
A protein scaffold, engineered SPINK2, for generation of inhibitors with high affinity and specificity against target proteases
Authors: Nishimiya Daisuke , Kawaguchi Yoshirou , Kodama Shiho , Nasu Hatsumi , Yano Hidenori , Yamaguchi Aya , Tamura Masakazu , Hashimoto Ryuji 
Published: August 2019

AbstractProteases are one of attractive therapeutic targets to play key roles in pharmacological action. There are many protease inhibitors in nature, and most of them structurally have cystine knot motifs. Their structures are favorable for recognition of active pockets of proteases, leading to the potent inhibition. However, they also have drawbacks, such as broad cross-reactivity, on the therapeutic application. To create therapeutic proteins derived from a disulfide-rich scaffold, we selected human serine protease inhibitor Kazal type 2 (SPINK2) through a scaffold screening, as a protein scaffold with requirements for therapeutic proteins. We then constructed a diverse library of the engineered SPINK2 by introducing random mutations into its flexible loop region with the designed method. By phage panning against four serine proteases, we isolated potent inhibitors against each target with picomolar KD and sub-nanomolar Ki values. Also, they exhibited the desired specificities against target proteases without inhibiting non-target proteases. The crystal structure of kallikrein related peptidase 4 (KLK4)-engineered SPINK2 complex revealed the interface with extensive conformational complementarity. Our study demonstrates that engineered SPINK2 can serve as a scaffold to generate therapeutic molecules against target proteins with groove structures.
Bcl11b controls odorant receptor class choice in mice
Authors: Enomoto Takayuki , Nishida Hidefumi , Iwata Tetsuo , Fujita Akito , Nakayama Kanako , Kashiwagi Takahiro , Hatanaka Yasue , Kondo Hiro , Kajitani Rei , Itoh Takehiko , Ohmoto Makoto , Matsumoto Ichiro , Hirota Junji 
Published: August 2019

AbstractEach olfactory sensory neuron (OSN) expresses a single odorant receptor (OR) gene from the class I or class II repertoire in mice. The mechanisms that regulate OR class choice in OSNs remain unknown. Here, we show that the transcription factor Bcl11b determines the OR class to be expressed in OSNs. Both loss- and gain-of-function analyses demonstrate that class I is a default fate of OSNs and that Bcl11b dictates a class II OR choice by suppressing the effect of the J-element, a class I-OR enhancer. We further demonstrate that OSN-specific genetic manipulations of Bcl11b bias the OR class choice, generating mice with “class I-dominant” and “class II-dominant” noses, which display contrasting innate olfactory behaviors to two distinct aversive odorants. Overall, these findings reveal a unique transcriptional mechanism mediating a binary switch for OR class choice that is crucial to both the anatomical and functional organization of the olfactory system.