bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Acute Increases in Intraocular Pressure on Corneal Pachymetry in Rabbit Eyes Treated with Timolol Maleate
Authors: Sánchez-Barahona Cristina , Bolívar Gema , Mikropoulos Dimitrios G. , Konstas Anastasios G. , Teus Miguel A. 
Published: January 2019

Objective:
To evaluate in an in vivo rabbit model, the effect of topical timolol maleate therapy on the central corneal thickness response to acute intraocular pressure increases.

Method:
In this prospective and interventional controlled study, the central corneal thickness and intraocular pressure were measured in vivo in 12 rabbit eyes treated with topical timolol maleate for 1 month and in 12 controls at baseline, and after the intraocular pressure (measured by direct cannulation of the anterior chamber) was increased to 15 and 30 mmHg using a forced saline infusion into the anterior chamber.

Results:
There were no significant differences in the basal central corneal thickness values (control group, 373.2±12.9 µm; study group, 377.5±19.2 µm, p=0.5) or the central corneal thickness values when the intraocular pressure was increased to 15 mmHg (control group, 335.2±14.3 µm; study group, 330.0±32.1 µm, p=0.6) and to 30 mmHg (study group, 318.8±25.3 µm; control group, 329.8±21.0 µm, p=0.3).

Conclusion:
Rabbit corneas treated with topical timolol maleate for 1 month did not show a strain response to acute intraocular pressure increases that differed from control eyes. This is in contrast to a previous finding in which rabbit eyes treated with prostaglandin analogues had a greater decrease in central corneal thickness in response to a sudden intraocular pressure increase compared with untreated corneas.
Preparation and In-vitro Evaluation of Levan Micelles: A Polyfructan Based Nano-carrier for Breast Cancer Targeted Delivery
Authors: Patel Parth , Agrawal Yadvendrakumar 
Published: January 2019

Background:
Levans are biopolymers of fructose, produced by different microorganisms.
Fructose present in the levan micelles binds with the Glucose Transporter 5 (GLUT 5) which is overexpressed
in the breast cancer cells.

Objective:
Increased solubility of paclitaxel by loading in the GLUT 5 transporter targeted levan-based
micelles may enhance its bioavailability and facilitate a targeted delivery to the breast cancer cells.

Methods and Results:
Critical micelle concentration of levan with an average molecular weight of
800,000 Dalton was found to be 0.125µM corresponding to 0.1mg/mL using pyrene I3/I1 method. At
critical micelle concentration (CMC), levan formed very mono-disperse (PDI-0.082) micellar particles
with a particle size of 153.1 ± 2.31nm and -14.6 ± 2mV zeta potential. In-vitro drug release study was
performed to identify the fit kinetic model along with Fourier transform infrared analysis and Differential
scanning calorimetry studies. In-vitro kinetic model fitting revealed first-order drug release from
the prepared micellar composition. The drug-loaded micellar composition was studied for its anticancer
activity in breast cancer cell line. The IC50 value obtained was 1.525 ± 0.11nM on MCF7 cell line.

Conclusion:
Paclitaxel micelles showed a nineteen-fold improvement in the IC50 value compared to
free paclitaxel. Hemocompatibility study was performed with a view to parenteral administration. This
solution containing drug was found to be hemocompatible when added to bovine blood in 1:4 ration.
Micelles are proven fairly compatible on the basis of hemolysis test results.
Pengetahuan Ibu, Dukungan Sosial, dan Dukungan Tenaga Kesehatan terhadap Keputusan Memberikan ASI Eksklusif
Authors: Mawaddah Shohipatul , Barlianto Wisnu , Nurdiana Nurdiana 
Published: January 2019

Abstrak Praktek di lapangan menunjukkan bahwa masih banyak ibu yang tidak memberikan ASI eksklusif. Provinsi Nusa Tenggara Barat (NTB) merupakan daerah yang berhasil memberikan ASI eksklusif. Wilayah Gangga memiliki cakupan terendah di NTB yaitu 30,4%. Penelitian ini mencoba menggali secara bersamaan prediktor munculnya masalah yang ada di wilayah Gangga yaitu berupa pengetahuan ibu, dukungan sosial baik dari suami, keluarga dan teman serta dukungan dari tenaga kesehatan. Desain penelitian observasional analitik cross-sectional digunakan dalam penelitian ini dengan memberikan kuesioner pada 165 responden yang memiliki bayi usia lebih dari 6-12 bulan. Hasil penelitian menunjukkan hanya 38,2% responden memutuskan memberikan ASI eksklusif di Gangga. Hasil uji regresi logistik bineri pada model terbaik menunjukkan variabel yang paling kuat berpengaruh berturut-turut adalah dukungan sosial suami (p=0,001), dukungan tenaga kesehatan (p=0,016), dukungan sosial keluarga (p=0,020) dan dukungan sosial teman (p=0,049). Sedangkan pengetahuan ibu (p=0,171) memiliki pengaruh yang tidak signifikan. Tingginya pengetahuan, besarnya dukungan sosial suami, keluarga, dan teman serta dukungan tenaga kesehatan secara simultan memiliki pengaruh terhadap keputusan ibu dalam memberikan ASI eksklusif.Kata Kunci : Pengetahuan, dukungan sosial, dukungan tenaga kesehatan, ASI eksklusifÂ Abstract The real practices show that there are still many mothers who do not give exclusive breastfeeding. West Nusa Tenggara (NTB) Province is an area that shows successful exclusive breastfeeding. Gangga area has the lowest coverage in NTB, as much as 30.4%. This research tried to simultaneously explore the predictors of the emergence of problems that exist in Gangga region in the form of motherâ€™s knowledge, social supports from husband, family, and friends, and also support from health workers. A cross-sectional observational analytic design was used in this study by providing questionnaires to 165 respondents who had babies older than 6-12 months. The results show only 38.2% of respondents in Gangga decide to give exclusive breastfeeding. From binary logistic regression test result in the best model, the strongest influential variables consecutively are husbandâ€™s social support (p=0.001), health personnel support (p=0.016, familyâ€™s social support (p=0.020) and friendâ€™s social support (p=0.049). While motherâ€™s knowledge (p=0.171) shows insignificant influence. The high level of knowledge, the amount of social support of husband, family, and friends, and support of health workers simultaneously have an influence on the mother's decision in giving exclusive breastfeeding. Keywords: exclusive breastfeeding, health personnel support, knowledge, social support
Evaluasi pemanfaatan laboratorium pada diklat pengendali hama dan penyakit ikan di Balai Diklat Aparatur Kementerian Kelautan dan Perikanan
Authors: Nugroho Fajar 
Published: January 2019

Keberadaan laboratorium menjadi hal yang penting dalam pelaksanaan Diklat Pengendali Hama dan Penyakit Ikan (PHPI) oleh karena itu Balai Diklat Aparatur (BDA) Kementerian Kelautan dan Perikanan (KKP) bekerjasama dengan Stasiun Karantina Ikan Kelas II Cirebon pada tahun 2014 membangun laboratorium uji karantina ikan di lingkungan kampus BDA KKP. Kajian ini bertujuan untuk mengevaluasi pemanfaatan laboratorium Diklat PHPI dengan mengukur perubahan kepuasan peserta terhadap ketersediaan media pembelajaran dan kelengkapan fasilitas belajar mengajar, nilai hasil belajar, peningkatan pengetahuan dan keterampilan peserta sebelum dan setelah laboratorium digunakan. Data diperoleh melalui pengisian angket/ kuesioner evaluasi pelaksanaan diklat kepada peserta. Validitas dan reabilitas instrumen telah diuji sehingga istrumen dapat digunakan dengan valid dan reliabel. Uji t dilakukan untuk mengetahui perbedaan nilai pengukuran sebelum dan sesudah laboratorium beroperasi. Hasil kajian menunjukkan bahwa semua indikator meningkat setelah operasionalisasi laboratorium di BDA. Secara statistik kepuasan peserta terhadap kelengkapan fasilitas pembelajaran dan keterampilan peserta meningkat signifikan. Adapun peningkatan pengetahuan peserta, peningkatan kepuasan peserta terhadap kelengkapan media pembelajaran dan peningkatan nilai hasil belajar peserta, secara statistik tidak meningkat signifikan.Kata kunci: evaluasi, diklat, laboratorium, pemanfaatan, PHPI THE EVALUATION OF LABORATORY UTILIZATION ON FISH QUARANTINE OFFICERS TRAINING IN MARINE AND FISHERIES OFFICERS TRAINING CENTER, MINISTRY OF MARINE AFFAIRS AND FISHERIESAbstractThe laboratory become inseparable in Fish Quarantine Officer Training. Therefore, Marine and Fisheries Officers Training Center (MFO TC) in collaboration with Cirebon Fish Quarantine Office build the fish quarantine laboratorium in MFO TC campus in 2014. This study was conducted to evaluate the lab utilitazion by measure the change of training participans satisfaction in learning media and facilities, participans final grade, and cognitive/knowledge and psychomotor skill enhancement before and after laboratory operationalization. Data were collected by distributed the evaluation questionnaire. The instruments were used in this study valid and reliable according to the result of the instrument’s validity and reability test. T-test two samples for means was used to analyse the differences of measurement before and after laboratory operationalization. Statistically, psychomotor skill enhancement and training participans satisfaction in facilities were significant increased after laboratory operationalization. Knowledge enhancement, training participans satisfaction in learning media and final grade were insignificant increased.Keywords: fish quarantine officer, laboratory, evaluating, utilization, training
Construct validity of creative thinking skills instrument for biology student teachers in the subject of human physiology
Authors: Pada Andi Ulfa Tenri , Mustakim Siti Salina , Subali Bambang 
Published: January 2019

This article discusses the construct validity of the creative thinking skill instrument supporting a conation idea aspect for biology student-teachers in the subject of Human Physiology. Two hundred and eighteen students participated in this study. Construct validity was obtained through Confirmatory Factor Analysis technique (CFA). Reliability was estimated by composite reliability. Findings show that the construct validity and the reliability of the instrument are high. A thorough discussion on the findings and future implications are provided towards the end of this paper.
Species Composition of Fruit Flies (Diptera: Tephritidae) in Sorong and Raja Ampat, West Papua
Authors: Linda Linda , Witjaksono Witjaksono , Suputa Suputa 
Published: January 2019

Fruit fly monitoring is an important part of integrated pest management since it provides information about fruit flies species composition in any given area. The aim of this study was to find out species composition of fruit fly in the City of Sorong, and both Sorong and Raja Ampat Regencies. Sampling was conducted from June to November 2016 using trapping method. There were 19 species of fruit flies in the whole sampling locations, consist of 11 species attracted to cue lure and 8 species attracted to methyl eugenol. Bactrocera frauenfeldi was the dominant species in trap baited with cue lure, while B. umbrosa and B. dorsalis were dominant in trap baited with methyl eugenol. Fruit fly diversity index in Raja Ampat Regency and City of Sorong were low while diversity index in Sorong Regency was relatively moderate. IntisariMonitoring lalat buah merupakan bagian penting dalam pengelolaan hama terpadu yang memberikan informasi tentang komposisi lalat buah di suatu area. Tujuan dari penelitian ini adalah untuk mengetahui komposisi spesies lalat buah di Kabupaten Sorong, kota Sorong dan Kabupaten Raja Ampat. Pengambilan sampel dilakukan mulai dari Juni sampai November 2016 dengan metode pemasangan perangkap. Total terdapat 19 spesies lalat buah dari seluruh wilayah pengambilan sampel, dengan 11 spesies yang tertarik cue lure dan 8 spesies tertarik metil eugenol. Bactrocera frauenfeldi adalah lalat buah yang paling dominan pada perangkap dengan atraktan cue lure sedangkan B. dorsalis serta B. umbrosa dominan pada perangkap dengan atraktan metil eugenol. Indeks keragaman jenis lalat buah di Kabupaten Raja Ampat dan Kota Sorong termasuk rendah sedangkan di Kabupaten Sorong indeks keragamannya relatif sedang.
Potensi Parasitoid Telur dalam Mengendalikan Wereng Batang Cokelat (Nilaparvata lugens Stal.) Pasca Ledakan Populasi di Kabupaten Banyumas
Authors: Minarni Endang Warih , Suyanto Agus , Kartini Kartini 
Published: January 2019

This study aims to determine the type and potency of egg parasitoid in controlling brown planthopper (Nilaparvata lugens Stal.) pests in Banyumas regency after the pest’s explosion. This research has been conducted in five districts of the endemic area of brown planthopper in Banyumas Regency, i.e. in Jatilawang, Cilongok, Kebasen, Sumpiuh, and Kembaran. Each of the districts was taken 5 sample villages. The testing and calculation of the level of parasitization were done in the laboratory of Plant Protection, Faculty of Agriculture, University of Jenderal Soedirman, Purwokerto. The research used nest plot design, where the first factor was the district and the second factor was the village. Village nested in district. The data were analyzed using F 5% test, followed by 5% DMRT, if there were any differences found . The results of the study were as follows: (1) The parasitoids found in Banyumas Regency were Gonatocerus sp. and Oligosita sp. with the ability to parasite 26.8−64.73%, and 1.82−31.40%; (2) the presence parasitoid has the potency to suppress the intensity of brown planthopper attack on the vegetative phase, the intensity of attacks ranged between 6.96−23.58%, with brown planthopper population ranged from 0.84 to 27.36 individuals per hill. IntisariPenelitian ini bertujuan untuk mengetahui jenis dan potensi parasitoid telur dalam mengendalikan hama wereng batang coklat (Nilaparvata lugens Stal.) di Kabupaten Banyumas pasca terjadinya ledakan. Penelitian ini dilaksanakan di lima kecamatan daerah endemik wereng batang cokelat di wilayah Kabupaten Banyumas yaitu Kecamatan Jatilawang, Cilongok, Kebasen, Sumpiuh, Kembaran. Masing-masing kecamatan diambil 5 desa sampel. Pengujian dan penghitungan tingkat pemarasitan dilakukan di laboratorium Perlindungan Tanaman Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto. Penelitian menggunakan rancangan petak tersarang, dengan kecamatan sebagai faktor pertama dan desa sebagai faktor kedua. Desa tersarang pada kecamatan. Data dianalisis menggunakan uji F 5%, apabila ada perbedaan dilanjutkan dengan uji banding ganda DMRT 5 %. Adapun hasil penelitian adalah sebagai berikut: (1) Parasitoid yang ditemukan di Kabupaten Banyumas adalah Gonatocerus sp. dan Oligosita sp. dengan kemampuan memarasit 26,8−64,73%, dan sebesar 1,82−31,40 %, (2) keberadaan parasitoid berpotensi menekan intensitas serangan hama wereng batang cokelat pada fase vegetatif, intensitas serangan berkisar antara 6,96−23,58%, dengan populasi wereng batang cokelat berkisar 0,84−27,36 individu per rumpun.
Perancangan Primer Spesifik Subspesies Berbasis Gen Endoglukanase untuk Deteksi Ralstonia syzygii subsp. syzygii
Authors: Trianom Bambang , Arwiyanto Triwidodo , Joko Tri 
Published: January 2019

Ralstonia syzygii subsp. syzygii that belong to Ralstonia solanacearum species complex is the cause of Sumateran disease of clove. The disease was reported to cause widespread devastation on clove plantings in Indonesia. One of the control strategies is to reduce the spread of the disease through early detection on clove seedlings. The study aimed to design the specific primers based on endoglucanase (egl) gene of R. syzygii subsp. syzygii as a tool for early diagnosis. The analyses were conducted on development of specific primers design using egl sequences retrieved from GenBank, Polymerase Chain Reaction (PCR), primers sensitivity and specificity test. The pair of primers UGMRss-F (5’-GCTCACCATCGC CAAGGACAGCG-3’) and UGMRss-R (5’-TTCGATCGAACGCCTGGTTGAGC-3’) could amplify R. syzygii subsp. syzygii at ~378 base pairs with 0.8 ng/µl minimum concentration of DNA. The primers was specific to R. syzygii subsp. syzygii but not to other bacterial species even in the same phylotype. IntisariRalstonia syzygii subsp. syzygii merupakan bakteri yang termasuk dalam kelompok Ralstonia solanacearum species complex yang menyebabkan penyakit Sumatera pada tanaman cengkih. Penyakit ini menyebabkan kerugian yang sangat besar dan sampai saat ini belum ditemukan cara pengendalian yang efektif. Salah satu upaya pencegahan penyakit adalah melalui deteksi dini dan mencegah penyebaran penyakit melalui peredaran bibit dari areal yang endemis. Penelitian ini bertujuan untuk merancang primer spesifik berbasis gen endoglukanase (egl) sebagai upaya deteksi dini penyakit Sumatera. Analisis yang dilakukan meliputi desain primer spesifik dengan menggunakan data sekuens gen egl dari GenBank, Polymerase Chain Reaction (PCR), uji kepekaan primer dan uji kekhususan primer. Desain primer yang berhasil dirancang terdiri dari UGMRss-F (5’- GCTCACCATCGCCAAGGACAGCG-3’) dan UGMRss-R (5’-TTC GATCGAACGCCTGGTTGAGC-3’) dengan amplikon ~378 pasang basa. Pada konsentrasi DNA 0,8 ng/µl, secara peka R. syzygii subsp. syzygii masih dapat teramplifikasi dengan baik. Primer ini juga hanya dapat mendeteksi R. syzygii subsp. syzygii dan tidak untuk bakteri lain bahkan pada filotipe yang sama.
Factores psicosociales del Bullying y las estrategias de afrontamiento como factor diferencial
Authors: SALAZAR FERNANDEZ MARISOL , Corredor Pinzón Nancy Eliana , GRANADA WILLIAM PANIAGUA , Trejos García Jenny , Valencia Puentes Adelaida 
Published: January 2019

El objetivo del presente estudio es identificar las estrategias de afrontamiento aprendidas desde la formación en el hogar, empleadas por los estudiantes de grado sexto de la Institución Educativa Técnica de Occidente de la ciudad de Tuluá, frente a la presentación del Bullying. Participaron 107 estudiantes, se empleó una adaptación del cuestionario sobre intimidación y maltrato entre iguales de Ortega, Mora-Merchán y Mora. (2009). Se evidenció que las estrategias de afrontamiento presentan íntima relación con la conformación familiar. El impacto del fenómeno del Bullying precisa la actuación conjunta tanto de las instituciones educativas con políticas y protocolos de manejo como la intervención de los actores del conflicto escolar, pero de igual forma, se hace necesario sensibilizar a las familias frente al papel que juegan no solo como soporte, sino también, como modeladores de conductas agresivas y de las respuestas ante las mismas.
Estrategias geométricas de configuraciones urbanas
Authors: Mariño Díaz Javier Alberto , Vergel Ortega Mawency , Delgado Rojas Julio Alfredo 
Published: January 2019

Este artículo toma como génesis las lógicas de las formas geométricas en la producción de arquitectura. En búsqueda de generar otras alternativas de composición geométrica, se proyecta todo un proceso de experimentación, análisis e investigación, en una serie de ejercicios aplicados y realizados por cada uno de los estudiantes, logrando canalizar el desarrollo evolutivo de nuevas soluciones tanto a la forma y estructura, como al manejo espacial a sentir. Si bien por tantos años diferentes escuelas se han regido solo al manejo tradicional de la composición geométrica, otros por lo contrario nos salimos de la tradición funcionalista, en donde esta vez, entender, diagnosticar y configurar cada uno de los factores directos e implícitos del lugar, conlleva a la interpretación de cada uno de los lenguajes encontrados. De aquí yace una metodología capaz de abordar las capacidades semiológicas, partiendo de lo más básico y simple para lograr construir lógicas geométricas de complejidad constructiva y aplicada. Se expresa la total capacidad de observación, se transfiere el significado existente en las formas actuales construidas de un lugar urbano, y aquí su condición sensible de percibir, analizar y conectar cada una de las vivencias transformadoras de un usuario social y cultural capaz de dinamizar cualquier espacio.
UMA ALTERNATIVA CURRICULAR NO CONTEXTO ESCOLAR BRASILEIRO: A Pedagogia Waldorf e a Integralidade do Conhecimento e do Ser
Authors: Albuquerque e Silva Caricelma Aparecida Lima , Costa Nayara Tatiana Santos da 
Published: January 2019

O presente trabalho trata sobre a educação e a integralidade do ser, no qual suscita-se a reflexão sobre como esta temática pode abrir caminhos alternativos para se pensar a noção e os desafios da formação humana. Como um desses caminhos, apresentamos a Pedagogia Waldorf, partindo da intenção de saber se a mesma contempla a integralidade do ser em sua proposta curricular. Portanto, objetivamos compreender como a integralidade humana aparece na proposta curricular da educação infantil na Escola Waldorf Recife e, para isso, conceituamos a integralidade humana a partir das ideias de Edgar Morin, descrevemos os princípios e fundamentos da Pedagogia Waldorf e investigamos como a integralidade é abordada na proposta curricular da educação infantil da referida escola. O percurso metodológico possui natureza qualitativa, do tipo exploratória, cujos dados foram construídos através de análise documental apoiada pela observação sistemática como instrumento de coleta de dados. A pesquisa evidenciou que a integralidade do ser aparece na proposta curricular da Pedagogia Waldorf plenamente harmonizada com o Paradigma Complexo, superando a fragmentação do conhecimento e do ser, harmonizando-se com a crítica à supervalorização da racionalidade, defendida por Edgar Morin.
UMA CRIANÇA CAÍDA DO CHÃO: contaminação e acoplamentos virais em currículos
Authors: Moreira de Oliveira Thiago Ranniery , Cassal Luan 
Published: January 2019

Um acoplamento viral com HIV é uma composição que põe à prova mecanismos como a temporalidade da reprodução sexual e a relacionalidade social. É esse campo que cruza política e sexualidade, matizado por graus de ansiedade e pânico que analisamos nesse artigo, explorando as dimensões da contaminação dentro um panorama mais amplo das políticas ecológicas. Inspirados nos debates sobre biopoder, propomos um experimento narrativo e ensaístico a partir trajetórias de crianças vivendo com HIV nas escolas, argumentando que a iminência de contaminação dos corpos nos currículos convida a pensar os entrelaçamentos entre vida e morte e vida e não-vida. Sugerimos, assim, o vírus como uma figura de poder que produz a materialização diferença como um erro metabólico do tempo da reprodução e da projeção do futuro. Defendemos que abordar a experiência dessas crianças oferece um suplemento às considerações (bio)políticas dos debates ambientais e das políticas ecológicas. O agenciamento viral coloca enredamentos existentes nos currículos entre sexualidade e ecologia política que tem recebido pouco crédito nos recentes debates sobre o Antropoceno. Esses laços complicam a imaginação ética da vida social ao expandir o corpo para além do humano quanto permitem abordar como e onde as disposições de poder dos currículos podem ser configuradas ao colabar os corpos como unidades separáveis em si mesmas e hermeticamente fechadas.