bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three Worlds of Representation: A Linkage-Based Typology of Parties in Western and Eastern Europe
Authors: Wineroither David M. , Seeber Gilg U. H. 
Published: April 2018

This article is part of the special cluster titled Parties and Democratic Linkage in Post-Communist Europe, guest edited by Lori Thorlakson, and will be published in the August 2018 issue of EEPS Have Eastern European democracies developed patterns of accountability similar to those existent in their established counterparts? While most accounts of convergence are confined to the world of programmatic reasoning and policy representation, we use a unique data set to cover the wealth of instrumental and emotional modes of linkage building. We apply advanced techniques of model-based cluster analysis to establish a linkage-based typology of political parties. In the East, the contrast of programmatic and clientelistic parties is most essential in the absence of strong regional subdivisions. In the West, the structure of linkage building is characterized by an all-encompassing divide that separates mainstream and challenger parties. Parties in Southern Europe form a distinct Mediterranean type of “machine politics.” The results for affluent post-industrial societies both support and contradict premises of the cartel party hypothesis. For third-wave democracies in the East, our results suggest the persistence of legacies of pre-communist and communist rule against the weight of cumulative democratic experience. In sum, patterns of accountability remained markedly different in the two regions on the eve of the economic crisis in 2008–2009.
Introduction to Special Section, “Parties and Party System Development in Post-communist Europe”
Authors: Thorlakson Lori 
Published: April 2018

This article is part of the special cluster titled Parties and Democratic Linkage in Post-Communist Europe, guest edited by Lori Thorlakson, and will be published in the August 2018 issue of EEPS This article introduces a special section on parties and democratic linkage in post-communist Europe. It sets out the main objectives and research questions that guide the four articles in this special section, presenting these in the context of the comparative politics literature on party and party system change and democratic development. It introduces the key arguments of the articles in the section, arguing that the contributions identify regionally distinctive patterns of party and party system behaviour in Central and Eastern Europe. These patterns have fuelled a quest for more suitable conceptual and measurement tools and call for diversity in comparative analysis, combining intra and inter regional comparison.
Orthopaedic In-Training Examination Resources and Residency Training for the Foot and Ankle Domain
Authors: Janney Cory F. , Kunzler Daniel , Safavi Pejma Shazadeh , Panchbhavi Vinod 
Published: April 2018

Background. Residency programs use the annual Orthopaedic In-Training Examination (OITE) prepared by the American Academy of Orthopaedic Surgeons (AAOS) to monitor resident progress and prepare them for the part 1 of the American Board of Orthopaedic Surgeons (ABOS) Certifying Examination. The purpose of this study was to determine resources residents currently use to prepare for the OITE and also to learn about their perception of training they receive in the foot and ankle subspecialty in their program and their interest in foot and ankle fellowship after residency. Methods. An anonymous survey was sent to both allopathic programs and osteopathic residents to learn what resources residents used to study for the OITE, preparatory question sets, on-call resources, their perception on training received in foot and ankle surgery, and their intent to pursue fellowship training. Results. A total of 130 residents participated in the survey. The majority of residents in allopathic and osteopathic residencies used Orthobullets (OB) to prepare for the OITE and use this resource while on-call. Most residents also used OB question sets to study along with the AAOS self-assessment examinations. In total, 43.2% of osteopathic residents felt they did not get enough exposure to foot and ankle subspecialty while in training, in contrast to 31.2% of allopathic residents. A total of 35% of all orthopaedic surgery residents felt they lacked enough exposure to foot and ankle orthopaedic surgery. Only 7 residents (6%, 6 allopathic, 1 osteopathic) intended to pursue a foot and ankle fellowship following graduation. Conclusion. Online resources such as OB continue to be frequently used by residents for preparation for the OITE. Greater than one-third of orthopaedic residents feel they do not get enough exposure to foot and ankle orthopaedic surgery. Improvement in this area could be helped by continued endeavors from the American Orthopaedic Foot and Ankle Society such as the Visiting Professor Program and Resident Scholarship Program. Levels of Evidence: Level V: Single Cross-Sectional Study
The use of RemoweLL oxygenator-integrated device in the prevention of the complications related to aortic valve surgery in the elderly patient: Preliminary results
Authors: Molardi Alberto , Di Chicco Maria V , Carino Davide , Goldoni Matteo , Ricci Matteo , Borrello Bruno , Gripshi Florida , Gherli Tiziano , Nicolini Francesco 
Published: April 2018

BackgroundThe effects of fat microembolization due to cardiopulmonary bypass are well known in cardiac surgery. Our aim is to evaluate the use of the RemoweLL device (Eurosets, Medolla, Italy) during elective aortic valve replacement in elderly patients (>70 years old) to rate its biochemical and clinical effects. The RemoweLL device is an oxygenator-integrated reservoir which combines two strategies for fat emboli and leucocytes removal: filtration and supernatant elimination.MethodsForty-four elderly patients were enrolled and assigned randomly to a Group A (standard device) and a Group B (RemoweLL). Biochemical effects were evaluated by blood samples, which were tested for white blood cells, neutrophils, protein SP-100 and interleukin 6 besides standard lab tests. Our clinical endpoints were any type of neurological, cardiac, respiratory, gastrointestinal or renal complications, and length of stay in the intensive care unit. Statistical analysis was carried out with chi square test for non-parametric data; t test and analysis of variance for repeated measures were used for parametric data.ResultsGroup B showed lower levels of white blood cells, neutrophils, interleukin 6 and protein SP-100 immediately and 24 hours after the operation. Group B also showed a lower amount of neurocognitive type II dysfunction even if the length of stay in the ICU did not change.ConclusionsThe RemoweLL system is safe and effective in reducing inflammatory response to cardiopulmonary bypass and it could be a useful tool in minimizing negative effects of cardiopulmonary bypass; however, it does not seem to have any effect on elderly patients’ hospital stay.
Studies on sporadic non-syndromic thoracic aortic aneurysms: 1. Deregulation of Jagged/Notch 1 homeostasis and selection of synthetic/secretor phenotype smooth muscle cells
Authors: Chiarini Anna , Onorati Francesco , Marconi Maddalena , Pasquali Alessandra , Patuzzo Cristina , Malashicheva Anna , Irtyega Olga , Faggian Giuseppe , Pignatti Pier F , Trabetti Elisabetta , Armato Ubaldo , Dal Pra Ilaria 
Published: April 2018

Background Sporadic non-syndromic thoracic aortic aneurysms (SNSTAAs) are less well understood than familial non-syndromic or syndromic ones. The study aimed at defining the peculiar morphologic and molecular changes occurring in the media layer of SNSTAAs. Design This study was based on a single centre design. Methods Media layer samples taken from seven carefully selected SNSTAAs and seven reference patients (controls) were investigated via quantitative polymerase chain reaction, proteomics-bioinformatics, immunoblotting, quantitative histology, and immunohistochemistry/immunofluorescence. Results In SNSTAAs media, aortic smooth muscle cells numbers were halved due to an apoptotic process coupled with a negligible cell proliferation. Cystathionine γ-lyase was diffusely up-regulated. Surviving aortic smooth muscle cells exhibited diverging phenotypes: in inner- and outer-media contractile cells prevailed, having higher contents of smooth-muscle-α-actin holoprotein (45-kDa) and of caspase-3-cleaved smooth-muscle-α-actin 25-kDa fragments; in mid-media, aortic smooth muscle cells exhibited a synthetic/secretor phenotype, down-regulating vimentin, but up-regulating glial fibrillary acidic protein, trans-Golgi network 46 protein, Jagged1 (172-kDa) holoprotein, and Jagged1’s receptor Notch1. Extracellular soluble Jagged1 (42-kDa) fragments accumulated. Conclusions In SNSTAAs, there is a relentless aortic smooth muscle cells attrition caused by the up-regulated cystathionine γ-lyase. In mid-media, synthetic/secretor aortic smooth muscle cells intensify Jagged1/NOTCH1 signalling in the attempt to counterbalance the weakened aortic wall, due to aortic smooth muscle cells net loss and mechanical stress. Synthetic/secretor aortic smooth muscle cells are apoptosis-prone, and the accruing thrombin-cleaved Jagged1 fragments counteract the otherwise useful effects of Jagged1/NOTCH1 signalling, thus hampering tissue homeostasis/remodelling, and aortic smooth muscle cells adhesion, differentiation, and migration.
Radiographic and Clinical Outcomes From the Use of S2 Alar Screws in Surgery for Adult Spinal Deformity
Authors: Nakazawa Toshiyuki , Inoue Gen , Imura Takayuki , Miyagi Masayuki , Saito Wataru , Shirasawa Eiki , Uchida Kentaro , Takahira Naonobu , Takaso Masashi 
Published: April 2018

Study Design: Retrospective. Objectives: To evaluate the efficacy of S2 alar screws in surgery for correction of adult spinal deformity (ASD). Methods: We retrospectively reviewed the cases of 23 patients (mean follow-up: 18.5 months, minimum 12 months) who underwent corrective surgery for ASD using S2 alar screws as anchors for instrumentation of lower vertebrae. The background of the patients and their spinopelvic parameters (pelvic incidence [PI], pelvic tilt [PT], lumbar lordosis [LL], thoracic kyphosis [TK], sagittal vertical axis [SVA], and PI-LL) were evaluated. Results: LL was improved from 9.7 ± 20.5° and SVA from 141.0 ± 64.0 mm before surgery to 39.0 ± 9.6° and 51.7 ± 40.8 mm immediately after surgery, respectively, and 38.2 ± 12.7° and 70.5 ± 59.2 mm at final follow-up. In 13 patients without sufficient correction (postoperative PI-LL ≥10°), bone mineral density and postoperative LL were significantly less, and PI, PI-LL, and PT were significantly greater than in patients with postoperative PI-LL <10°, suggesting that these are risk factors for undercorrection. In 5 patients, SVA increased more than 40 mm during follow-up. Postoperative LL was significantly less (31.4° vs 41.0°) and postoperative PI-LL was significantly greater (21.6° vs 9.3°) in these patients, suggesting a PI-LL mismatch induces postoperative progression of global malalignment. Conclusions: Use of S2 alar screws as anchors for instrumentation in ASD surgery should be restricted. Their use might be an option for patients with low PI, and without severe osteoporosis, in whom efficient surgical correction can be obtained.
Avoidable Emergency Department Utilization Within 6 Months Following Elective Thoracolumbar Spine Surgery for Degenerative Pathologies
Authors: Flood Meghan V. , Manson Neil A. , Green Alana J. , Abraham Edward P. , Bigney Erin 
Published: April 2018

Study Design: Retrospective study. Objective: To identify prevalence of, reasons for, and predictors of emergency department (ED) utilization 6 months following elective thoracolumbar spine surgery. Methods: A retrospective review of a patient database was conducted (N = 577). Visits were divided by orthopedic spinal surgeons into (1) avoidable ED visit, (2) appropriate/no visit, and (3) unrelated visit. Measures: Demographics, pain scores, patient characteristics, and surgical factors. Results: A total of 14.38% of patients made an ED visit the majority for avoidable reasons (11.43%). Avoidable ED visits were predominately attributed to pain (45.5%) and physiology-related issues (50.0%). Significant differences in the Numerical Rating Scale–leg pain (NRS-L); U = 13 931, P = .031) were found. Patients with avoidable visits had higher leg pain prior to surgery than those without an avoidable visit. Marital status was also statistically significant, χ2(2, N = 535) = 8.189, P = .017. Patients were more likely to make an avoidable postoperative ED visit if they were either single or divorced/separated compared to patients who were married. A multivariate logistic regression model including NRS-L and marital status was statistically significant, χ2(3) = 10.14, P = .017; however only explained 3.7% of the variance. Conclusion: A large percentage of elective thoracolumbar surgery patients returned to the ED within 6 months for avoidable reasons. Patients likely to make avoidable visits could not be identified prior to surgery in a clinically meaningful way. Reasons for patients returning to the ED for avoidable reasons focused on pain management and minor physiological symptoms. Enhanced presurgical education may manage postsurgical expectations helping to prevent avoidable ED visits.
Beef, Fish, and Chestnut Cake: Food for Heroes in the Late Imperial Chinese Novel
Authors: Liang Yan 
Published: April 2018

Abstract
This article explores the functions that food, as a narrative trope, has played in the characterization and storytelling of three prominent martial arts novels in late imperial China: Shuihu zhuan 水滸傳 (Water Margin), Sanxia wuyi 三俠五義 (Three Gallant Men and Five Loyal Brothers), and Ernü yingxiong zhuan 兒女英雄傳 (A Tale of Lovers and Heroes). The detailed and individualized descriptions of the heroes' eating and drinking add depth and complexity to the characters and justify plot development. Meanwhile, the narrative functions of the food descriptions in these three novels elucidate the distinct challenges for the martial arts novel in character portrayal and narrative focus.
Tolerance to paternal genotoxic damage promotes survival during embryo development in zebrafish (Danio rerio)
Authors: Fernández-Díez Cristina , González-Rojo Silvia , Lombó Marta , Herráez M. Paz 
Published: April 2018

ABSTRACT
Spermatozoa carry DNA damage that must be repaired by the oocyte machinery upon fertilization. Different strategies could be adopted by different vertebrates to face the paternal genotoxic damage. Mammals have strong sperm selection mechanisms and activate a zygotic DNA damage response (DDR) (including cell cycle arrest, DNA repair and alternative apoptosis) in order to guarantee the genomic conformity of the reduced progeny. However, external fertilizers, with different reproductive strategies, seem to proceed distinctively. Previous results from our group showed a downregulation of apoptotic activity in trout embryos with a defective DNA repairing ability, suggesting that mechanisms of tolerance to damaged DNA could be activated in fish to maintain cell survival and to progress with development. In this work, zebrafish embryos were obtained from control or UV-irradiated sperm (carrying more than 10% of fragmented DNA but still preserving fertilization ability). DNA repair (γH2AX and 53BP1 foci), apoptotic activity, expression of genes related to DDR and malformation rates were analyzed throughout development. Results showed in the progeny from damaged sperm, an enhanced repairing activity at the mid-blastula transition stage that returned to its basal level at later stages, rendering at hatching a very high rate of multimalformed larvae. The study of transcriptional and post-translational activity of tp53 (ZDF-GENE-990415-270) revealed the activation of an intense DDR in those progenies. However, the downstream pro-apoptotic factor noxa (ZDF-GENE-070119-3) showed a significant downregulation, whereas the anti-apoptotic gene bcl2 (ZDF-GENE-051015-1) was upregulated, triggering a repressive apoptotic scenario in spite of a clear genomic instability. This repression can be explained by the observed upregulation of p53 isoform Δ113p53, which is known to enhance bcl2 transcription. Our results showed that tp53 is involved in DNA damage tolerance (DDT) pathways, allowing the embryo survival regardless of the paternal DNA damage. DDT could be an evolutionary mechanism in fish: tolerance to unrepaired sperm DNA could introduce new mutations, some of them potentially advantageous to face a changing environment.
Histone deacetylases 1 and 2 regulate the transcriptional programs of nephron progenitors and renal vesicles
Authors: Liu Hongbing , Chen Shaowei , Yao Xiao , Li Yuwen , Chen Chao-Hui , Liu Jiao , Saifudeen Zubaida , El-Dahr Samir S. 
Published: April 2018

ABSTRACT
Nephron progenitor cells (NPCs) are Six2-positive metanephric mesenchyme cells, which undergo self-renewal and differentiation to give rise to nephrons until the end of nephrogenesis. Histone deacetylases (HDACs) are a group of epigenetic regulators that control cell fate, but their role in balancing NPC renewal and differentiation is unknown. Here, we report that NPC-specific deletion of Hdac1 and Hdac2 genes in mice results in early postnatal lethality owing to renal hypodysplasia and loss of NPCs. HDAC1/2 interact with the NPC renewal regulators Six2, Osr1 and Sall1, and are co-bound along with Six2 on the Six2 enhancer. Although the mutant NPCs differentiate into renal vesicles (RVs), Hdac1/2 mutant kidneys lack nascent nephrons or mature glomeruli, a phenocopy of Lhx1 mutants. Transcriptional profiling and network analysis identified disrupted expression of Lhx1 and its downstream genes, Dll1 and Hnf1a/4a, as key mediators of the renal phenotype. Finally, although HDAC1/2-deficient NPCs and RVs overexpress hyperacetylated p53, Trp53 deletion failed to rescue the renal dysgenesis. We conclude that the epigenetic regulators HDAC1 and HDAC2 control nephrogenesis via interactions with the transcriptional programs of nephron progenitors and renal vesicles.
Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam
Authors: Thi Ty Hang Vu , Thi Han Ny Nguyen , My Phuc Tran , Thi Thanh Tam Pham , Thao Huong Dang , Dang Trung Nghia Ho , Tran Anh Vu Nguyen , Thi Hong Phuong Pham , Van Xang Nguyen , Dong Nguyen , Nhu Hiep Pham , Van Hung Nguyen , Tinh Hien Tran , Rabaa Maia , Thwaites Guy E. , Baker Stephen , Van Tan Le , van Doorn H.Rogier ,   VIZIONS consortium
Published: April 2018

Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam.Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, sensitivity of the Luminex against reference assays was 91.8%, 95% CI 88.1-94.7 (270/294), whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents.Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription.
Human CCL3L1 copy number variation, gene expression, and the role of the CCL3L1-CCR5 axis in lung function
Authors: Adewoye Adeolu B. , Shrine Nick , Odenthal-Hesse Linda , Welsh Samantha , Malarstig Anders , Jelinsky Scott , Kilty Iain , Tobin Martin D. , Hollox Edward J. , Wain Louise V. 
Published: April 2018

Background: The CCL3L1-CCR5 signaling axis is important in a number of inflammatory responses, including macrophage function, and T-cell-dependent immune responses. Small molecule CCR5 antagonists exist, including the approved antiretroviral drug maraviroc, and therapeutic monoclonal antibodies are in development. Repositioning of drugs and targets into new disease areas can accelerate the availability of new therapies and substantially reduce costs. As it has been shown that drug targets with genetic evidence supporting their involvement in the disease are more likely to be successful in clinical development, using genetic association studies to identify new target repurposing opportunities could be fruitful. Here we investigate the potential of perturbation of the CCL3L1-CCR5 axis as treatment for respiratory disease. Europeans typically carry between 0 and 5 copies of CCL3L1 and this multi-allelic variation is not detected by widely used genome-wide single nucleotide polymorphism studies. Methods: We directly measured the complex structural variation of CCL3L1 using the Paralogue Ratio Test and imputed (with validation) CCR5d32 genotypes in 5,000 individuals from UK Biobank, selected from the extremes of the lung function distribution, and analysed DNA and RNAseq data for CCL3L1 from the 1000 Genomes Project. Results: We confirmed the gene dosage effect of CCL3L1 copy number on CCL3L1 mRNA expression levels. We found no evidence for association of CCL3L1 copy number or CCR5d32 genotype with lung function. Conclusions: These results suggest that repositioning CCR5 antagonists is unlikely to be successful for the treatment of airflow obstruction.