bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reliable surface modification of dental plastic substrates to reduce biofouling with a photoreactive phospholipid polymer
Authors: Ishihara Kazuhiko , Fukazawa Kyoko , Inoue Yuuki , Koyama Jun , Mori Yoshiyuki , Kinoshita Toru , Hiranuma Katsumi , Yasuda Noboru 
Published: April 2018

ABSTRACTPlastic substrates have been widely applied in clinical settings for dental treatments. These substrates should be strong enough for long‐term implantation in the oral cavity and should be resistant to biofouling. We developed a new photoreactive phospholipid polymer to reduce biofouling on dental plastics via a photochemical reaction. Poly(methyl methacrylate) (PMMA) and poly(ether ether ketone) (PEEK) were used as dental plastics. To determine the antibiofouling properties on the polymer surface, the phospholipid polymer was covalently immobilized on the substrates by UV irradiation. We evaluated the antibiofouling properties by observing the protein adsorption and cell and bacterial adhesion. Significant protein adsorption and cell adhesion appeared on the bare PMMA and PEEK substrates but decreased dramatically after surface modification with the phospholipid polymer. Thus, this photoreactive polymer shows potential for conferring dental plastics with antibiofouling properties. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46512.
Analysis of the ‘Dutch Disease’ Effect on the Selected Resource‐Rich ASEAN Economies
Authors: Taguchi Hiroyuki , Khinsamone Soukvisan 
Published: April 2018

AbstractThis article examines the applicability of the Dutch Disease hypothesis by using a vector auto‐regression model, focusing on the resource‐rich and middle‐income economies in the Association of Southeast Asian Nations. The empirical study found that the latecomers of Lao People's Democratic Republic and Myanmar seemed to suffer from the Dutch Disease over the sample period for 1970–2015; and the forerunners of Indonesia and Malaysia, on the other hand, appeared to have no Dutch Disease effect at least in the current period of 1997–2015, although Indonesia had experienced the Dutch Disease in the previous period of 1970–1996. The lessons from the forerunners' experiences in order for the latecomers to escape from the Dutch Disease are to establish some funding system of allocating resource revenues for investment projects; to diversify domestic industries through improving business environments; and to improve institutional quality to reinforce resource governance.
Developing the Digital Kyoto Collection in Education and Research
Authors: Hill Mark Anthony 
Published: April 2018

ABSTRACTThe Kyoto embryo collection was begun in 1961 by Dr. Hideo Nishimura. The collection has been continuously developed and currently contains over 44,000 human normal and abnormal specimens. Beginning online in 1997, the internet provided an opportunity to make embryos from the collection widely available for research and educational purposes (http://tiny.cc/Embryo). These embryonic development resources have been continuously published and available from that time until today. Published in Japanese as an Atlas of Embryonic Development. Published online as the Kyoto Human Embryo Visualization Project (http://atlas.cac.med.kyoto-u.ac.jp) and also as the Human Embryo Atlas (http://tiny.cc/Human_Embryo_Atlas). Published now electronically as a digital eBook (http://tiny.cc/Kyoto_Collection_eBook). This new digital format allows incorporation of whole embryo and histology manipulable images, labels, and a linked glossary. New imaging modalities of magnetic resonance imaging (MRI) and episcopic fluorescence image capture (EFIC) can also be easily displayed as animations. For research, the collection specimens and histological sections have been extensively studied and published in several hundred papers, discussed here and elsewhere in this special edition. I will also describe how the Kyoto collection will now form a major partner of a new international embryology research group, the Digital Embryology Consortium (https://human-embryology.org). The digital Kyoto collection will be made available for remote researcher access, analysis, and comparison with other collections allowing new research and educational applications. This work was presented at the 40th Anniversary Commemoration Symposium of the Congenital Anomaly Research Center, Graduate School of Medicine, Kyoto University, Japan, November, 2015. Anat Rec, 301:998–1003, 2018. © 2018 Wiley Periodicals, Inc.
The Beginning of Kyoto Collection: Focus on Memories
Authors: Yasuda Mineo 
Published: April 2018

ABSTRACTThe early history of Kyoto Collection of Human Embryos and Fetuses was briefly described including reasons for planning the collection, ethical considerations, procedures for the collection, procedures for obtained specimens, research grants, participating members, and Professor Nishimura's Motto Anat Rec, 301:951–954, 2018. © 2018 Wiley Periodicals, Inc.
Convergent Synthesis of a Bisecting N‐Acetylglucosamine (GlcNAc)‐Containing N‐Glycan
Authors: Manabe Yoshiyuki , Shomura Hiroki , Minamoto Naoya , Nagasaki Masahiro , Takakura Yohei , Tanaka Katsunori , Silipo Alba , Molinaro Antonio , Fukase Koichi 
Published: April 2018

AbstractThe chemical synthesis of a bisecting N‐acetylglucosamine (GlcNAc)‐containing N‐glycan was achieved by a convergent synthetic route through [4+2] and [6+2] glycosylations. This synthetic route reduced the number of reaction steps, although the key glycosylations were challenging in terms of yields and selectivities owing to steric hindrance at the glycosylation site and a lack of neighboring group participation. The yields of these glycosylations were enhanced by stabilizing the oxocarbenium ion intermediate through ether coordination. Glycosyl donor protecting groups were explored in an effort to realize perfect α selectivity by manipulating remote participation. The simultaneous glycosylations of a tetrasaccharide with two disaccharides was investigated to efficiently construct a bisecting GlcNAc‐containing N‐glycan.
Big brother or harbinger of best practice: Can lecture capture actually improve teaching?
Authors: Joseph‐Richard Paul , Jessop Tansy , Okafor Godwin , Almpanis Timos , Price Daran 
Published: April 2018

Lecture capture is used increasingly in the UK, and has become a normal feature of higher education. Most studies on the impact of lecture capture have focused on benefits to student learning, the flipped classroom or student non‐attendance at lectures following its introduction. It is less clear how the use of lecture capture has impacted on lecturers’ own academic practice. In this study, we use a mixed‐methods approach to explore the impact of this intrusive yet invisible technology on the quality of teaching. We have mapped our findings to the UK Professional Standards Framework (UKPSF). In doing so, our data paints a mixed picture of lecture capture's Janus‐faced reality. On the one hand, it enhances lecturer self‐awareness, planning and conscious ‘performance’; on the other hand, it crushes spontaneity, impairs interaction and breeds wariness through constant surveillance. While the Teaching Excellence Framework rewards institutions for providing state‐of‐the‐art technology and lecture recording systems, our findings pose awkward questions as to whether lecture capture is making teaching more bland and instrumental, albeit neatly aligned to dimensions of the UKPSF. We provide contradictory evidence about lecture capture technology, embraced by students, yet tentatively adopted by most academics. The implications of our study are not straightforward, except to proceed with caution, valuing the benefits but ensuring that learning is not dehumanised through blind acceptance at the moment we press the record button.
Synthesis and luminescence properties of novel 8‐hydroxyquinoline derivatives and their Eu(III) complexes
Authors: Wu Yongqiang , Guo Tiantong , Shu Dehua , Zhang Wu , Luan Fangfei , Shi Ling , Guo Dongcai 
Published: April 2018

AbstractSix novel 8‐hydroxyquinoline derivatives were synthesized using 2‐methyl‐8‐hydroxyquinoline and para‐substituted phenol as the main starting materials, and were characterized by 1H nuclear magnetic resonance (NMR), mass spectrometry (MS), ultraviolet (UV) light analysis and infra‐red (IR) light analysis. Their complexes with Eu(III) were also prepared and characterized by elemental analysis, molar conductivity, UV light analysis, IR light analysis, and thermogravimetric–differential thermal analysis (TG–DTA). The results showed that the ligand coordinated well with Eu(III) ions and had excellent thermal stability. The structure of the target complex was EuY1–6(NO3)3.2H2O. The luminescence properties of the target complexes were investigated, the results indicated that all target complexes had favorable luminescence properties and that the introduction of an electron‐donating group could enhance the luminescence intensity of the corresponding complexes, but the addition of an electron‐withdrawing group had the opposite effect. Among all the target complexes, the methoxy‐substituted complex (–OCH3) had the highest fluorescence intensity and the nitro‐substituted complex (–NO2) had the weakest fluorescence intensity. The results showed that 8‐hydroxyquinoline derivatives had good energy transfer efficiency for the Eu(III) ion. All the target complexes had a relatively high fluorescence quantum yield. The fluorescence quantum yield of the complex EuY3(NO3)3.2H2O was highest among all target complexes and was up to 0.628. Because of excellent luminescence properties and thermal stabilities of the Eu(III) complexes, they could be used as promising candidate luminescent materials.
Contributions of depth filter components to protein adsorption in bioprocessing
Authors: Khanal Ohnmar , Singh Nripen , Traylor Steven J. , Xu Xuankuo , Ghose Sanchayita , Li Zheng J. , Lenhoff Abraham M. 
Published: April 2018

AbstractDepth filtration is widely used in downstream bioprocessing to remove particulate contaminants via depth straining and is therefore applied to harvest clarification and other processing steps. However, depth filtration also removes proteins via adsorption, which can contribute variously to impurity clearance and to reduction in product yield. The adsorption may occur on the different components of the depth filter, that is, filter aid, binder, and cellulose filter. We measured adsorption of several model proteins and therapeutic proteins onto filter aids, cellulose, and commercial depth filters at pH 5–8 and ionic strengths <50 mM and correlated the adsorption data to bulk measured properties such as surface area, morphology, surface charge density, and composition. We also explored the role of each depth filter component in the adsorption of proteins with different net charges, using confocal microscopy. Our findings show that a complete depth filter's maximum adsorptive capacity for proteins can be estimated by its protein monolayer coverage values, which are of order mg/m2, depending on the protein size. Furthermore, the extent of adsorption of different proteins appears to depend on the nature of the resin binder and its extent of coating over the depth filter surface, particularly in masking the cation‐exchanger‐like capacity of the siliceous filter aids. In addition to guiding improved depth filter selection, the findings can be leveraged in inspiring a more intentional selection of components and design of depth filter construction for particular impurity removal targets.
Three‐dimensional collagenous niche and azacytidine selectively promote time‐dependent cardiomyogenesis from human bone marrow‐derived MSC spheroids
Authors: Joshi Jyotsna , Mahajan Gautam , Kothapalli Chandrasekhar R. 
Published: April 2018

AbstractEndogenous adult cardiac regenerative machinery is not capable of replacing the lost cells following myocardial infarction, often leading to permanent alterations in structure‐function‐mechanical properties. Regenerative therapies based on delivering autologous stem cells within an appropriate 3D milieu could meet such demand, by enabling homing and directed differentiation of the transplanted cells into lost specialized cell populations. Since type I collagen is the predominant cardiac tissue matrix protein, we here optimized the 3D niche which could promote time‐dependent evolution of cardiomyogenesis from human bone marrow‐derived mesenchymal stem cells (BM‐MSC). 3D collagen gel physical and mechanical characteristics were assessed using SEM and AFM, respectively, while the standalone and combined effects of collagen concentration, culture duration, and 5‐azacytidine (aza) dose on the phenotype and genotype of MSC spheroids were quantified using immunofluorescence labeling and RT‐PCR analysis. Increasing collagen concentration led to a significant increase in Young's modulus (p < 0.01) but simultaneous decrease in the mean pore size, resulting in stiffer gels. Spheroid formation significantly modulated MSC differentiation and genotype, mostly due to better cell–cell interactions. Among the aza dosages tested, 10 μM appears to be optimal, while 3 mg/ml gels resulted in significantly lower cell viability compared to 1 or 2 mg/ml gels. Stiffer gels (2 and 3 mg/ml) and exposure to 10 μM aza upregulated early and late cardiac marker expressions in a time‐dependent fashion. On the other hand, cell–cell signaling within the MSC spheroids seem to have a strong role in influencing mature cardiac markers expression, since neither aza nor gel stiffness seem to significantly improve their expression. Western blot analysis suggested that canonical Wnt/β‐catenin signaling pathway might be primarily mediating the observed benefits of aza on cardiac differentiation of MSC spheroids. In conclusion, 2 mg/ml collagen and 10 μM aza appears to offer optimal 3D microenvironment in terms of cell viability and time‐dependent evolution of cardiomyogenesis from human BM‐MSCs, with significant applications in cardiac tissue engineering and stem cell transplantation for regenerating lost cardiac tissue.
Stable miRNA overexpression in human CAP cells: Engineering alternative production systems for advanced manufacturing of biologics using miR‐136 and miR‐3074
Authors: Weis Benjamin L. , Guth Nadine , Fischer Simon , Wissing Silke , Fradin Simon , Holzmann Karl‐Heinz , Handrick René , Otte Kerstin 
Published: April 2018

AbstractChinese hamster ovary (CHO) cells still represent the major production host for therapeutic proteins. However, multiple limitations have been acknowledged leading to the search for alternative expression systems. CEVEC's amniocyte production (CAP) cells are human production cells demonstrated to enable efficient overexpression of recombinant proteins with human glycosylation pattern. However, CAP cells have not yet undergone any engineering approaches to optimize process parameters for a cheaper and more sustainable production of biopharmaceuticals. Thus, we assessed the possibility to enhance CAP cell production capacity via cell engineering using miRNA technology. Based on a previous high‐content miRNA screen in CHO‐SEAP cells, selected pro‐productive miRNAs including, miR‐99b‐3p, 30a‐5p, 329‐3p, 483‐3p, 370‐3p, 219‐1‐3p, 3074‐5p, 136‐3p, 30e‐5p, 1a‐3p, and 484‐5p, were shown to act pro‐productive and product independent upon transient transfection in CAP and CHO antibody expressing cell lines. Stable expression of miRNAs established seven CAP cell pools with an overexpression of the pro‐productive miRNA strand. Subsequent small‐scale screening as well as upscaling batch experiments identified miR‐136 and miR‐3074 to significantly increase final mAb concentration in CAP‐mAb cells. Transcriptomic changes analyzed by microarrays identified several lncRNAs as well as growth and apoptosis‐related miRNAs to be differentially regulated in CAP‐mAb‐miR‐136 and ‐miR‐3074. This study presents the first engineering approach to optimize the alternative human expression system of CAP‐cells.
Nationwide trends in the incidence and outcome of patients with gastrointestinal stromal tumour in the imatinib era
Authors: van der Graaf W T A , Tielen R , Bonenkamp J J , Lemmens V , Verhoeven R H A , de Wilt J H W 
Published: April 2018

Abstract

Background
The incidence, treatment and outcome of patients with newly diagnosed gastrointestinal stromal tumour (GIST) were studied in an era known for advances in diagnosis and treatment.

Methods
Nationwide population-based data were retrieved from the Netherlands Cancer Registry. All patients with GIST diagnosed between 2001 and 2012 were included. Primary treatment, defined as any treatment within the first 6–9 months after diagnosis, was studied. Age-standardized incidence was calculated according to the European standard population. Changes in incidence were evaluated by calculating the estimated annual percentage change (EAPC). Relative survival was used for survival calculations with follow-up available to January 2017.

Results
A total of 1749 patients (54·0 per cent male and median age 66 years) were diagnosed with a GIST. The incidence of non-metastatic GIST increased from 3·1 per million person-years in 2001 to 7·0 per million person-years in 2012; the EAPC was 7·1 (95 per cent c.i. 4·1 to 10·2) per cent (P &lt; 0·001). The incidence of primary metastatic GIST was 1·3 per million person-years, in both 2001 and 2012. The 5-year relative survival rate increased from 71·0 per cent in 2001–2004 to 81·4 per cent in 2009–2012. Women had a better outcome than men. Overall, patients with primary metastatic GIST had a 5-year relative survival rate of 48·2 (95 per cent c.i. 42·0 to 54·2) per cent compared with 88·8 (86·0 to 91·4) per cent in those with non-metastatic GIST.

Conclusion
This population-based nationwide study found an incidence of GIST in the Netherlands of approximately 8 per million person-years. One in five patients presented with metastatic disease, but relative survival improved significantly over time for all patients with GIST in the imatinib era.
Prognostic significance of gross extrathyroidal extension invading only strap muscles in differentiated thyroid carcinoma
Authors: Park S Y , Kim H I , Kim J-H , Kim J S , Oh Y L , Kim S W , Chung J H , Jang H W , Kim T H 
Published: April 2018

Abstract

Background
In the eighth edition of the AJCC staging system for differentiated thyroid carcinoma (DTC), minimal extrathyroidal extension (ETE) is no longer a determinant of T3 category. Instead, gross ETE invading only strap muscles has been designated as a new T3b category. The long-term prognosis of patients with DTC and gross ETE invading only strap muscles was investigated.

Methods
This was a retrospective analysis of patients who underwent thyroidectomy between 1996 and 2005. Differences in cancer-specific and recurrence-free survival according to extent of ETE were assessed.

Results
A total of 3174 patients with DTC were included. No significant differences were observed in 10-year cancer-specific survival among patients with no ETE (98·6 per cent), microscopic ETE (98·3 per cent) and gross ETE invading only strap muscles (98·9 per cent) (P = 0·375). The 10-year recurrence-free survival rate for patients with gross ETE invading only strap muscles (89·2 per cent) was shorter than that for patients with no ETE (93·7 per cent; P = 0·016), but similar to that of patients with microscopic ETE (90·3 per cent). In univariable analysis, patients with gross ETE invading only strap muscles had a significantly higher risk of recurrence than those with no ETE (hazard ratio (HR) 1·67, 95 per cent c.i. 1·10 to 2·55; P = 0·017). In multivariable analysis, gross ETE invading only strap muscles was not an independent predictor of recurrence (HR 1·09, 0·71 to 1·69; P = 0·685).

Conclusion
Although gross ETE invading only strap muscles may provide prognostic information about long-term recurrence, it does not affect mortality. The actual impact of gross ETE invading only strap muscles will be important in revising the staging system in the future.
Firm sustainable development and stakeholder engagement: The role of government support
Authors: Quan Yi , Wu Huiying , Li Sihai , Ying Sammy Xiaoyan 
Published: April 2018

AbstractThis study examines the sustainable development of Chinese firms in the context of the government implementing environmental policies that support green initiatives, from a resource‐based perspective. Using a sample of Chinese listed firms in the most polluting industries during the period 2001–2015, we find a positive relationship between environmental performance and economic performance. We also find that government‐controlled resources, including bank loans and tax benefits, are the mechanisms through which the effects of environmental investments are transformed into economic gains. Further analyses show that the positive association between environmental and economic performance holds for state‐owned firms only. These results indicate that although “being green” is associated with improvements in firm performance, such a strategy benefits only those firms that receive preferential treatment from the government. This study reveals that the Chinese Government, as the most influential stakeholder, plays an important role in determining the impact of environmental performance on economic performance.
MiR‐21 improves invasion and migration of drug‐resistant lung adenocarcinoma cancer cell and transformation of EMT through targeting HBP1
Authors: Su Chongyu , Cheng Xu , Li Yunsong , Han Yi , Song Xiaoyun , Yu Daping , Cao Xiaoqing , Liu Zhidong 
Published: April 2018

AbstractThis study was aimed at the investigation of the effects of miR‐21 on drug resistance, invasion, migration, and epithelial–mesenchymal transition (EMT) of lung adenocarcinoma cells and the related molecular mechanisms. Cell viability of A549 cell line was measured by MTT assay. Wound healing assay and transwell assay were, respectively, employed to examine cell migration and invasion abilities. The cells were transfected with miR‐21 mimic or inhibitor using Lipofectamine 3000. The target relationship between miR‐21 and HBP1 was confirmed by luciferase reporter gene assay. Western blot and qRT‐PCR were used to examine the expression of HBP1 and EMT‐related molecules. Compared with A549 cells, drug resistance of A549/PTX cells and A549/DDP cells were obviously stronger. A549/PTX cells and A549/DDP cells had stronger ability of migration and invasion compared with parental A549 cells. Meanwhile, EMT of A549/PTX and A549/DDP was significantly higher than that of A549 cells. MiR‐21 promoted migration, invasion, and EMT of human lung adenocarcinoma cancer cells. Our experiment also verified the target relationship between miR‐21 and HBP1. MiR‐21 may affect migration and invasion ability of drug‐resistant lung adenocarcinoma cells by targeting HBP1, therefore modulating EMT.