bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disagreement Predicts Startup Success: Evidence from Venture Competitions
Authors: Gius Luca 
Published: November 2024

This paper examines the relationship between disagreement surrounding a startup proposition and its future success. I find that the more venture competition judges disagree on the quality of a startup, the more likely the startup is to succeed, particularly when its proposition is unique. To explain this correlation, I build on the notion that (i) entrepreneurs pursue opportunities based on their subjective beliefs and (ii) common opinion alone cannot be a source of competitive advantage. Therefore, value is disproportionately created and captured by founders with unconventional ideas that spark disagreement, and potential investors should harness disagreement as a predictor of success. I leverage data from 67 venture competitions to show that the empirical implications of this theoretical framework are supported by the data, whereas alternative explanations (e.g., that judges disagree more about risky ventures) are not. Additionally, I provide insights into which evaluators tend to disagree more often (e.g., former entrepreneurs) and which aspects of a startup (e.g., business model) are most polarizing. This work has broad implications for investors and institutions that strive to evaluate the potential of startup ideas. Supplemental Material: The online appendix is available at https://doi.org/10.1287/stsc.2024.0169 .
A single dose recombinant AAV based CHIKV vaccine elicits robust and durable protective antibody responses in mice
Authors: Zhu Qin-Xuan , Zhang Ya-Nan , Zhang Hong-Qing , Leng Chao , Deng Cheng-Lin , Wang Xin , Li Jia-Jia , Ye Xiang-Li , Zhang Bo , Li Xiao-Dan 
Published: November 2024

Background
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that is responsible for Chikungunya fever, which is characterized by fever, rash, and debilitating polyarthralgia. Since its re-emergence in 2004, CHIKV has continued to spread to new regions and become a severe health threat to global public. Development of safe and single dose vaccines that provide durable protection is desirable to control the spread of virus. The recombinant adeno-associated virus (rAAV) vectors represent promising vaccine platform to provide prolonged protection with a single-dose immunization. In this study, we developed a rAAV capsid serotype 1 vector based CHIKV vaccine and evaluated its protection effect against CHIKV challenge.

Methodology
The recombinant AAV1 encoding the full-length structural proteins of CHIKV (named as rAAV1-CHIKV-SP) was generated in vitro by transfecting the plasmids of AAV helper-free system into HEK-293T cells. The safety and immunogenicity of rAAV1-CHIKV-SP were tested in 4-week-old C57BL/6 mice. The antibody responses of the mice receiving prime-boost or single-dose immunization of the vaccine were determined by ELISA and plaque reduction neutralizing test. The immunized mice were challenged with CHIKV to evaluate the protection effect of the vaccine.

Conclusions
The rAAV1-CHIKV-SP showed remarkable safety and immunogenicity in C57BL/6 mice. A single dose intramuscular injection of rAAV1-CHIKV-SP elicited high level and long-lasting antibody responses, and conferred complete protection against a heterologous CHIKV strain challenge. These results suggest rAAV1-CHIKV-SP represents a promising vaccine candidate against different CHIKV clades with a simplified immunization strategy.
Comparative analysis of whole exome sequencing kits for the canine genome
Authors: Jang Jinhee , Lee Yong-Jik , Ko Soohyun , Abd El-Aty A. M. , Gecili Ibrahim , Jeong Ji Hoon , Kwon ChangHyuk , Jung Tae Woo 
Published: November 2024

Objectives
As the public’s interest in companion dogs grows, health issues in these animals are also emerging, necessitating the optimization of whole exome sequencing (WES) as a valuable method for disease prediction. While WES targeting the human genome is well established, WES targeting the canine genome is understudied, and there is a need to find effective analysis kits.

Methods
We compared and analyzed the performance of three WES kits from Twist and Agilent using the canine genome as the target to perform genetic analysis of canine diseases effectively. The levels of total reads, the duplication rate, and variant calling in canine genomic DNA samples from seven healthy dogs (three beagles, one bichon fry, one maltese, one welsh corgi, and one mixed breed) without any interventions were examined through WES via Twist and Agilent kits.

Results
We found that while Twist had the lowest total read number, the number of reads in the SSXT series was significantly (P<0.05) greater. Twist showed low evenness and high standard deviation, but the SSXT series showed relatively high evenness. Compared with Twist, the SSXT series from a depth of 30× presented a significantly (P<0.05) greater target ratio. Among the four kits, the significantly lowest duplicate ratio was confirmed for SSXT (O/N) (30% lower than Twist).

Conclusion
The most important performance of the kit, the number of variants detected, was 48,302 for Twist and 130,506 for SSXT (O/N). On the basis of the performance comparison results, SSXT (O/N) was found to have the best performance.
Radiosurgery-induced early changes in peritumoral tissue sodium concentration of brain metastases
Authors: Ruder Arne Mathias , Mohamed Sherif A. , Hoesl Michaela A. U. , Neumaier-Probst Eva , Giordano Frank A. , Schad Lothar , Adlung Anne 
Published: November 2024

Background
Stereotactic radiosurgery (SRS) is an effective therapy for brain metastases. Response is assessed with serial 1H magnetic resonance imaging (MRI). Early markers for response are desirable to allow for individualized treatment adaption. Previous studies indicated that radiotherapy might have impact on tissue sodium concentration. Thus, 23Na MRI could provide early quantification of response to SRS.

Purpose
We investigated whether longitudinal detection of tissue sodium concentration alteration within brain metastases and their peritumoral tissue after SRS with 23Na MRI was feasible.

Study type
Prospective.

Population
Twelve patients with a total of 14 brain metastases from various primary tumors.

Assessment
23Na MRI scans were acquired from patients 2 days before, 5 days after, and 40 days after SRS. Gross tumor volume (GTV) and healthy-appearing regions were manually segmented on the MPRAGE obtained 2 days before SRS, onto which all 23Na MR images were coregistered. Radiation isodose areas within the peritumoral tissue were calculated with the radiation planning system. Tissue sodium concentration before and after SRS within GTV, peritumoral tissue, and healthy-appearing regions as well as the routine follow-up with serial MRI were evaluated.

Statistical tests
Results were compared using Student’s t-test and correlation was evaluated with Pearson’s correlation coefficient.

Results
We found a positive correlation between the tissue sodium concentration within the peritumoral tissue and radiation dosage. Two patients showed local progression and a differing tissue sodium concentration evolution within GTV and the peritumoral tissue compared to mean tissue sodium concentration of the other patients. No significant tissue sodium concentration changes were observed within healthy-appearing regions.

Conclusion
Tissue sodium concentration assessment within brain metastases and peritumoral tissue after SRS with 23Na MRI is feasible and might be able to quantify tissue response to radiation.
Competitiveness and quality conditions of animal products in Croatia compared to the Cefta and EC countries
Authors: BOŽIČ M. , JURIĆ I. , KOLEGA A. , ĐIKIĆ Marija , KRALIK Gordana , PETRIČEVIĆ A. 
Published: November 2024

Capacity of animal products production in Croatia was examined and compared to the same production in EU and CEFTA countries. Significant lagging behind of this production per capita was established in comparison to compared countries. Competitiveness of animal products from Croatia is significantly lower compared to surrounding countries, because of high imput costs and even more because of lower quality of products. Regulation for pork quality assessment on slaughterline was implemented in Croatia which is identical to EU regulation Nr. 3220/84 from 1984. Other regulations, for assessment of milk quality, animal feeds and slaughtered cattle in first line are currently under construction. Analyzed pork quality according to S-EUROP system showed that pig population in Croatia, examined in Papuk - Požega region, have meatiness of 50.1 % which could be taken as accurate assessment of pig population in Croatia produced for market. Examined ABCD Hypor pig population had meatiness of 56.1 % and pigs from “Dubravica” farm obtained by mating of Swedish Landrace and C and AB lines had meatiness between 53 and 54 %. Quantity and fat content of milk was also examined. Quantity of milk increases, which is important because Croatia is importer of large amounts of milk.
Factors influencing the competitiveness of Hungarian agriculture and rural areas prior to joining the EU
Authors: JUHÁSZ G. , KEMÉNY G. , MÁTHÉ A. 
Published: November 2024

At present a state of crisis is characteristic of Hungarian agriculture, and, together with it, of the rural areas. As much as 60 % of those previously employed in the agricultural sector, 607,000 employees, have lost their jobs. Since agriculture is closely linked to rural life, it is in the rural areas that the resultant problems primarily occur. One third of the population of Hungary lives in the rural areas, but unemployment in these areas constitutes a half of the total number of unemployed in the country. During the privatisation process the land areas of Hungary were divided up to a great extent. The area of most of the individual farms is under one hectare. A substantial proportion of small plots, having been displaced from the produce supply market, now serve only the subsistence requirements of their owners. A large proportion of the agricultural structures still in operation and their successors can no longer serve the function which they previously fulfilled in their given region, while the stimulation of state intervention required is still awaited.
VARIABILITY OF THE CHARACTERISTICS OF HOGS BETWEEN SOWS WITHIN THE BOAR
Authors: ĐIKIĆ Marija , JURIĆ I. , MUNJAS D. 
Published: November 2024

Investigation was carried out on hogs of ABCD (n=23) Hypor hybrid produced by mating AB boar with five CD sows of Hypor parent line. Groups of offspring were marked as: A, B, C, D and E. The analyzed characteristics were production traits and on slaughtering line measured meatness by method “two points” (Regulations NN 79/95). Results of hogs within the boar for the analyzed characteristics were as follows: slaughtering weight 100.6 kg, age at slaughtering 195.6 days, weight of warm carcass 79.6 kg. The life daily gains were: of slaughtering weight 513.3 g, of carcass 407.0 g, and muscular tissue in carcass 224.9 g. On the slaughtering line following values were measured; backfat thickness 15.9 mm, muscle thickness of m.l.d. 69.1 mm, muscular tissue in carcass 56.4 %. Statistically significant difference on 1 % level was found for the percentage of muscular tissue in carcass and classes of the hogs between sows D 65.4 % (S) and E 60.7 % (S) in relation to A 53.8 % (U) and B 50.8 % (U), while difference between sow C 56.7 % (E) and B 50.8 % (U) and D 65.4 % (S) and E 60.7 % (S) were at 5 % level. Estimation of the variability of hog classes on the slaughhtering line implies that of carcass classes can also be influenced by the technological procedures through elimination of sows on the basis of their performance test.
A NEW METHOD FOR THE QUANTITATIVE DETERMINATION OF PROTEIN OF BACTERIAL ORIGIN ON THE BASIS OF D-AMINO ACID CONTENT
Authors: CSAPÓ J. , SCHMIDT J. , KISS-CSAPÓ Zsuzsanna , HOLLÓ I. , VISI-VARGA Éva , BALLA-TERLAKY Éva 
Published: November 2024

In the past years several methods have been developed for the determination of the proportion which is of microbial origin of the nitrogen-containing substances passed from the rumen into the abomasum or the small intestine. Recently, on examining the D-amino acid content of foodstuffs, particularly milk and milk products, it has been observed that, in addition to D-Ala, D-glutamic acid (D-Glu) and D-aspartic acid (D-Asp) can also be detected in similar quantities, primarily in products which have links with bacterial activity. This gave rise to the idea of examining the DAPA, D-Glu and D-Asp content of bacteria extracted from the rumen of cattle and that of chyme from the same cattle, in order to determine the type of relation existing among these three components, and to establish whether D-Asp and D-Glu can be used in the estimation of protein of bacterial origin. On determination of the DAPA, D-Asp and D-Glu content by means of amino acid analyser and high performance liquid chromatography of duodenal chyme from five growing bulls and of ruminal bacteria from the same bulls, the following values were established. For chyme (and, in brackets, for ruminal bacteria) r value calculated by means of linear regression was 0.778 (0.758) between DAPA and D-Asp, and 0.703 (0.808) between DAPA and D-Glu. The r values between the crude protein content of ruminal bacteria and the markers examined were found to be the following: DAPA, 0.737; D-Asp, 0.7254; D-Glu, 0.614. In the model experiment performed for the re-obtaining of values for protein of bacterial origin the theoretical values were determined on the basis of D-Asp and D-Glu and values approximately 10 % higher than the theoretical value on the basis of DAPA. It is therefore recommended that in addition to DAPA these other two amino acids be included among the bacterial protein markers.
THE EFFICIENCY OF DIFFERENT AMOUNT OF CATTLE DUNG ON SOIL MICROORGANISMS AIMING AT FARM ENVIRONMENT PROTECTION
Authors: MILAKOVIĆ Zlata , BUKVIĆ Ž. , BRKIĆ Suzana , ZIMMER R. , EMERT R. 
Published: November 2024

An exact vegetation experiment regarding investigation of efficiency of different liquid amount enriched with technological liquid waste on prevalent microbiological associations in soil type loessive semigley was set out at a farm in Eastern Croatia during 1997.The experiment variants were as follows: 1. liquid manure with disinfectants in the following doses: 1 l/m2 (30 kg N/ha), 1.5 l/m2 (45 kg N/ha), 2 l/m2 (60 kg N/ha), 3 l/m2 (90 kg N/ha); 6.5 l/m2 (195 kgN/ha); 2. NPK (180:110:160 kg/ha); 3. control - nonfertilized soil. Amounts of liquid farmyard manure enriched with disinfectants applied into tilth soil layer are very significant for condition and activity of physiological groups of microorganisms .Thus, smaller doses (1-3 l/m2) cause lower and relatively short term decrease of aminoheterotrophies, aminoautotrophies and asymbiotic nitrogen fixers (A. chroococcum). Higher doses (6.5 l/m2) cause stronger and long term decrease of the same physiological groups of microorganisms.
CORRELATION OF FEED CONVERSION WITH WEIGHT GAIN AND CHICKENS MORTALITY
Authors: BODAKOŠ D. , BUKVIĆ Ž. , BOGUT I. , MILAKOVIĆ Zlata 
Published: November 2024

Feed costs participate with over 50 % in a price per kilogram of fattened chicken. Each impact affecting increase, i.e. feed consumption decrease is very important regarding economic aspect. Mortality as one of the factors affecting feed conversion indicated varying largely in shifts of the same sector whereas varying from 5.62 % (sector I) to 7.40 % (sector III) was at the whole farm in our investigation. Calculated coefficient of simple correlation proved that there is correlation between feed conversion and mortality percentage at the end of fattening period in all sectors and for the farm averagely. Each kilogram of fattened chickens live weight is more expensive by higher feed amount. It makes every kilogram of fattened chickens more expensive by 0.048 kg at 5.61 % mortality whereas it increases 0.065 kg at 7.40 % mortality which causes higher feed consumption and economically more expensive fattening.
EFFECT OF FEED RESTRICTION DURING THE LAYING PERIOD ON THE REPRODUCTIVE PERFORMANCES OF GEESE KEPT UNDER INTENSIVE CONDITIONS
Authors: BOGENFÜRST F. 
Published: November 2024

In a 2 x 2-factor experiment: ad libitum [1], ad libitum+methionine supplementation [2], 220 g/day restriction [3] and 220 g/day restriction+methionine supplementation [4], 108 females and 36 males geese were housed for the laying period. Composition of the feed was: 11.5 MJ ME/kg, 15.5 % crude protein, 0.6 Met+Cys and 0.35 % Met. Methionine supplementation increased the Met-level by 0.2 % and the Met+Cys-level by 0.2 %. The average egg production decreased due to the feed restriction {65.6 eggs/layer [3] vs. 72.7 eggs/layer [1]}, but with methionine supplementation we were able to compensate for this parameter {70 eggs/layer [4]}. The results show that restriction has a positive effect on fertility {91.6 % [3] vs. 88.3 % [1]}. Using feed restriction the geese achieved lower weight gain. Feed restriction during egg production can be a suitable method to improve the reproduction parameters in geese, but further investigations are necessary.
EFFECTS OF FIVE PROTEIN LEVELS ON REPRODUCTIVE TRAITS OF
Authors: MELEG I. , HORN P. , DUBLECZ K. , VINCZE L. 
Published: November 2024

The experiment was conducted to study the effects of five feeding systems based on different (12, 14, 16, 18 and 20 %) protein levels, on the reproductive traits of utility-type pigeons. 30 breeding pairs per treatment were kept in pairs in a specially designed three tier cage system, in a windowless environmentaly controlled house at a constant 12 hour light period throughout the year. Using different protein levels failed to affect the length of egg cycle and egg weight of breeding pairs. Parallel with the increasing of crude protein content of diets, decreased the mortality and increased the weaning weight of squabs. Annual squab production and feed intake of parents became also higher. In spite of feed consumption of adults increased, feed conversion ratio of squabs decreased with the increase of protein content.
The future of milk and meat production in Slovenia
Authors: OSTERC Jože , ČEPIN Slavko , KLOPČIČ Marija , ČEPON Marko , ŽGUR Silvester , ŠTUHEC Ivan , KOMPAN Drago , HOLCMAN Antonija , LEVSTIK Snežana 
Published: November 2024

The production of milk and meat in Slovenia in the recent years and its future is presented. Market production of milk and meat has been increased as well as consumption. The rates of self-sufficiency are 123 % in cow's milk, 114 % in poultry meat, 97 % in beef, 82 % in pork and 100 % in freshwater fish. Three kg of milk and one kg of meat of sheep and goats and 0.3 kg meat of rabbit are also consumed per capita. Because of restricted natural conditions and environmental sensitivity as well as preservation of fertile soil sustainable animal production should be introduced. Special attention will be paid to ethics, which includes ethology and ecology. Despite requirements of more sustainable animal production meat and milk production could be augmented.
Future production of milk and meat in Croatia
Authors: ĐIKIĆ Marija , JURIĆ Ivan 
Published: November 2024

Production of animal products in Croatia does not meet the demand of consumption by citizens and tourism. Import of animal products grows, while, in the same time, utilization of agricultural land is only at 39 %. These trends set the conditions for the development strategy to stimulate the growth of animal production. For Croatia, accepting the standards of international conventions already signed and the European Union (EU) directives will probably mean slowing down the animal production growth. However, in Croatia there is a dominating opinion that all the legislation should be in accordance to the standards of EU countries, especially in tourism, as strategically most important branch of economy.
More on the relation between meat percentage and meat quality traits in swine carcasses
Authors: KRALIK Gordana , PETRIČEVIĆ Antun , ŠKRTIĆ Zoran , KUŠEC Goran 
Published: November 2024

This study involved 302 swine carcasses, dissected in the aim of their classification into (S)EUROP classes on the basis of relative share of muscle tissue. Following measures have been taken on the primarily processed swine carcasses: length, ham index, muscle and fat areas of m. longissimus dorsi, fat to meat ratio. The meat quality traits measured were: pH1, pH2, water holding capacity (w.h.c.) and colour. The differences in carcass quality indicators between pigs from different classes were statistically significant in the most of the cases (P < 0.05) and highly significant (P < 0.01). The analysis of meat quality traits showed that the meat had mostly normal characteristics, although it was also observed that swine carcasses from lower meat yield had more favourable quality traits than the carcasses with higher meat yield.
Percentage and distribution of tissues in pig carcasses in relation to (S)EUROP classes
Authors: PETRIČEVIĆ Antun , KRALIK Gordana , TOLUŠIĆ Zdravko , ŠKRTIĆ Zoran , KUŠEC Goran 
Published: November 2024

Four hundred of primarily processed carcasses of pigs with different genetic background and production conditions were included in this study. The average carcass mass was 80.43 kg (from 78.29 kg in class S to 88.34 kg in class O). The average lean meat percentage in carcasses was 54.31 %. According to classification (Pravilnik o utvrđivanju..., 1999) in S class were classified 10.25 %, 37.50 % in class E, 32.50 % in class U, 16.75 % in class R, 3.00 % in class O, while in class P there were no carcasses classified, which presents a satisfactory structure. By the dissection of the carcasses according to Weniger et al. (1963) into the main parts (ham, loin, belly-rib part, shoulder, neck) the shares of these parts became obvious. The differences in percentages of the main parts within classes were in favour of the higher classes, but statistically significant differences were established only in the case of ham (P < 0.01) belly-rib part (P < 0.01 and P < 0.05) and shoulder (P < 0.01 and P < 0.05). The percentages of lean meat in individual main parts, according to classification, were different in the most of the cases at the level of P < 0.01 and always in the favour of higher classes (S, E and U). Fat was almost always in negative proportion to the degree of meatiness. The percentages of bones showed relatively small differences between classes regarding the individual parts (P < 0.05) as well as the carcasses where differences were established at the levels of P < 0.01 and P < 0.05. In addition to presented results, the procedure for price determination according to lean meat percentage, i.e. classes were proposed.