bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of prediction models for carbapenem-resistant Klebsiella pneumoniae acquisition and prognosis in adult patients
Authors: Yao Huijuan , Yang Yu , Yao Huimin , Bu Shuhong , Li Lixia , Wang Fang , Zhang Jian , Chen Jihui 
Published: November 2024

ObjectivesTo explore the risk factors and clinical outcomes of carbapenem-resistant Klebsiella pneumoniae (CRKP) infection and establish nomograms to predict the probability of CRKP infection and mortality in adult patients.MethodsPatients infected with KP from August 2019 to April 2021 in a tertiary hospital in Shanghai were enrolled. Risk factors associated with CRKP and 30-day mortality were identified using multivariate logistic regression analysis and Cox regression analysis.ResultsOverall, 467 patients with KP infection were enrolled, wherein 210 (45.0%) patients were infected with CRKP and 257 (55.0%) patients with carbapenem-susceptible K. pneumoniae (CSKP). Five factors, namely Charlson’s Comorbidity Index (CCI) ≥ 3, the use of central venous catheterization, prior hospitalization during the 3 months before infection, and previous exposure to carbapenems and broad-spectrum β-lactams, were found to be independently associated with CRKP infection. Based on these parameters, the nomogram showed a better performance as indicated by C-index of 0.94 (95% confidence interval [CI]: 0.92–0.96) and well-fitted calibration curves. CRKP was independently associated with 30-day mortality. Multivariate Cox regression analysis revealed that age ≥65 years, higher CCI scores, higher Sequential Organ Failure Assessment scores, the presence of respiratory failure, albumin levels ≤30 g/L, and non-appropriate treatments in 3 days, were associated with 30-day mortality.ConclusionThe predictive nomogram established in this study can facilitate the clinicians to make better clinical decisions when treating patients with KP infection.
Chaihu Shugan powder restores fatty acid synthesis to alleviate insulin resistance in metabolic syndrome by regulating the LXRα/SREBP-1 signaling pathway
Authors: Lei Sisi , Peng Weihang , Wu Lulu , Yu Liyuan , Wang Meida , Li Qingmin , Deng Yi , Zhao Shuai , Huang Peiying , Chen Bojun 
Published: November 2024

BackgroundMetabolic syndrome (MS) is a significant risk factor for cardiovascular and cerebrovascular diseases, primarily driven by insulin resistance (IR). Although the herbal compound Chaihu Shugan powder (CSP) has demonstrated the potential to improve IR in animal models of MS, its mechanism of action remains incompletely understood. Therefore, this study aimed to investigate the biological pathways through which CSP exerts its therapeutic effects on IR in MS using both in vitro and in vivo methods.MethodsThe primary metabolites of CSP aqueous extract and CSP-containing serum were measured by LC-MS/MS. A mouse model of MS-related IR was induced by a high-fat, high-fructose diet combined with chronic immobilization stress. The CSP’s therapeutic potential was evaluated through glucose and insulin tolerance tests and hepatic insulin signaling molecules (p-IRS-1, IRS-1, p-Akt, and Akt). The expression of lipid metabolism-related factors (FFA, DAG, LXRα, SREBP-1, FASN, and ACC) in the liver was also measured. Hepatocyte IR was modeled using high-glucose and high-insulin conditions, and CSP impact was evaluated using 2-NBDG uptake and insulin signaling molecule expression. The specific mechanism of CSP was explored using the LXRα agonist T0901317.ResultsThe MS-related IR model exhibited a decreased p-Akt/Akt ratio and increased fasting glucose, insulin, homeostatic model assessment of IR, and hepatic lipid metabolism factors. Treatment with CSP mitigated these effects. In the hepatocyte IR model, CSP-containing serum improved glucose uptake and modulated the expression of insulin signaling and lipid metabolism factors. Furthermore, T0901317 reversed the beneficial effects of CSP, indicating the role of LXRα in CSP’s therapeutic action.ConclusionThe CSP ameliorated IR in MS by restoring fatty acid metabolism through the regulation of the LXRα/SREBP-1 signaling pathway.
Knowledge domain and trends in treatment-resistant schizophrenia (TRS) research based on CiteSpace bibliometrics analysis
Authors: Cai Haipeng , Du Ruonan , Zhang Jianyi , Wang Xin , Li Wei , Yang Kebing , Wang Zhiren 
Published: November 2024

BackgroundAlthough the number of studies on treatment-resistant schizophrenia (TRS) has been increasing, the global research hotspots and future research trends have not yet been established.ObjectiveThis study identify the hotspots of TRS research and predict future research trends using a bibliometric analysis.MethodsThe Web of Science Core Collection was searched using the keyword “TRS”, econometric and co-occurrence analyses were conducted using CiteSpace and VOSviewer software, and the results were visualised. PRISMA reporting guidelines were used for this study.ResultsIn total, 912 publications were included in the analysis. The number of publications on TRS has shown an increasing trend over the past 20 years. The United States and University of London were the countries and institutions with the highest total number of publications, respectively. Schizophrenia Research was the journal with the highest number of articles. American Journal of Psychiatry was the most cited journal. Based on the results of this analysis, cognitive impairment, clozapine-resistant schizophrenia, early-onset schizophrenia, and early recognition of TRS will be hotspots for future research in this field.ConclusionThere has been an upward trend in the number of publications on TRS each year. However, issues such as how to use antipsychotics more efficiently to treat TRS and how to predict the emergence of TRS as early as possible are still in urgent need of research and are current challenges for clinicians. The results of this study not only predict and analyse future research hotspots but also help researchers identify appropriate research directions and partners.
Exploring the mechanism of sesamin for the treatment of PM2.5-induced cardiomyocyte damage based on transcriptomics, network pharmacology and experimental verification
Authors: Zhang Yadong , Wen Rui , Ren Jingyi , Zhang Fan , Pei Huanting , Zuo Jinshi , Ma Yuxia 
Published: November 2024

IntroductionExposure to fine particulate matter (PM2.5) is known to be associated with cardiovascular diseases. Sesamin (Ses) is a natural phenolic compound found in sesame seeds and sesame oil. Ferroptosis is a novel mode of cell death characterised by iron-dependent lipid peroxidation. This study aims to explore whether PM2.5 can induce ferroptosis in H9C2 cells and to investigate the precise protective mechanism of Ses.MethodsBased on transcriptomic data, PM2.5 may induce ferroptosis in cardiomyocytes. The ferroptosis inducer erastin and ferroptosis inhibitor ferrostatin-1 (Fer-1) were used to illustrate the mechanisms involved in PM2.5-induced H9C2 cell injury. Using network pharmacology, the pharmacological mechanism and potential therapy targets of Ses were explored for the treatment of PM2.5-induced cardiomyocyte injury. H9C2 cells were cultured and pretreated with Fer-1 or different concentrations of Ses, and then cardiomyocyte injury model was established using erastin or PM2.5. Indicators of oxidative responses, including total superoxide dismutase, reduced glutathione, glutathione peroxidase and malondialdehyde, were measured. The expression levels of ferroptosis-related proteins were determined through Western blot analysis.ResultsResults demonstrate that PM2.5 induces ferroptosis in H9C2 cells and Ses exerts a protective effect by suppressing ACSL4-mediated ferroptosis.DiscussionOverall, these findings elucidate a novel mechanism by which Ses ameliorates the detrimental effects of PM2.5 on cardiomyocytes.
Long-term hypoxia modulates depolarization activation of BKCa currents in fetal sheep middle cerebral arterial myocytes
Authors: Nelapudi Nikitha , Boskind Madison , Hu Xiang-Qun , Mallari David , Chan Michelle , Wilson Devin , Romero Monica , Albert-Minckler Eris , Zhang Lubo , Blood Arlin B. , Wilson Christopher G. , Puglisi Jose Luis , Wilson Sean M. 
Published: November 2024

IntroductionPrevious evidence indicates that gestational hypoxia disrupts cerebrovascular development, increasing the risk of intracranial hemorrhage and stroke in the newborn. Due to the role of cytosolic Ca2+ in regulating vascular smooth muscle (VSM) tone and fetal cerebrovascular blood flow, understanding Ca2+ signals can offer insight into the pathophysiological disruptions taking place in hypoxia-related perinatal cerebrovascular disease. This study aimed to determine the extent to which gestational hypoxia disrupts local Ca2+ sparks and whole-cell Ca2+ signals and coupling with BKCa channel activity.MethodsConfocal imaging of cytosolic Ca2+ and recording BKCa currents of fetal sheep middle cerebral arterial (MCA) myocytes was performed. MCAs were isolated from term fetal sheep (∼140 days of gestation) from ewes held at low- (700 m) and high-altitude (3,801 m) hypoxia (LTH) for 100+ days of gestation. Arteries were depolarized with 30 mM KCl (30K), in the presence or absence of 10 μM ryanodine (Ry), to block RyR mediated Ca2+ release.ResultsMembrane depolarization increased Ry-sensitive Ca2+ spark frequency in normoxic and LTH groups along with BKCa activity. LTH reduced Ca2+ spark and whole-cell Ca2+ activity and induced a large leftward shift in the voltage-dependence of BKCa current activation. The influence of LTH on the spatial and temporal aspects of Ca2+ sparks and whole-cell Ca2+ responses varied.DiscussionOverall, LTH attenuates Ca2+ signaling while increasing the coupling of Ca2+ sparks to BKCa activity; a process that potentially helps maintain oxygen delivery to the developing brain.
The relationship between distress tolerance and life satisfaction among young adults in Saudi Arabia
Authors: Abd Ellatif Elsayed Hala , Aleriani Fatemah 
Published: November 2024

PurposeThis study examined the relationship between Distress Tolerance, defined as the individual’s ability to withstand psychological stress or endure negative emotions, and Life Satisfaction, the cognitive component of the individual’s subjective wellbeing, among Saudi young adults aged 20–30. The study aimed to understand the overall scores of distress tolerance, life satisfaction, and gender differences in these variables.MethodsOnline questionnaires were distributed to 348 participants selected based on inclusion/exclusion criteria, using self-report scales: the Distress Tolerance Scale (DTS) and the Satisfaction with Life Scale (SWLS). The sample consisted of 77 males and 271 females, with a mean age of 1.8793 years (SD = 0.81931). Data were analyzed using the SPSS program.ResultsThe findings supported our hypotheses that (1) Saudi young adults have high overall scores of distress tolerance and life satisfaction, (2) there is a significant positive correlation between distress tolerance and life satisfaction, (3) there are no significant differences between males and females regarding overall distress tolerance and life satisfaction levels, although (4) a significant gender difference was found in the emotional regulation subscale of DTS. A linear regression analysis also showed that distress tolerance significantly predicts life satisfaction.ConclusionDistress tolerance and life satisfaction are positively associated among Saudi young adults, with no significant gender differences in overall levels. However, differences in specific subscales, such as emotional regulation, warrant further investigation. These findings provide valuable insights for interventions to enhance this population’s wellbeing.
Treatment strategies for smartphone addiction: efficacy study of transcranial direct current stimulation and exergaming
Authors: Chen Jun , Jia YuQing , Zhu YanXia , Liu Qin , Cheng Fan , Yang Bo , Zhang EnMing 
Published: November 2024

ObjectiveSmartphone addiction is on the rise globally. This study aimed to compare the rehabilitative effects of transcranial direct current stimulation (tDCS) and exergames on smartphone addiction among college students. Additionally, we discussed the central mechanisms through changes in electroencephalography (EEG) to provide clinical insights.MethodsThirty-six participants were randomly assigned to three groups: control group, tDCS group (bilateral dorsolateral prefrontal cortex stimulation), and exergame group. The intervention lasted for 4 weeks with twice-weekly sessions. Outcome measures included the Smartphone Addiction Scale - Chinese Version (SAS-C), Iowa Gambling Task (IGT) net scores, and event-related potential (ERP) data collected during the IGT, focusing on P300 and feedback-related negativity (FRN) amplitudes.ResultsAll groups showed significant reductions in SAS-C scores post-intervention. Behaviorally, post-intervention scores improved, indicating significant effects of different interventions on participants’ strategy choices. P300 amplitudes increased significantly at outcome electrode sites for all groups, with the most notable increase in tDCS group FC2 and CP1. FRN amplitudes decreased significantly post-intervention in the control and tDCS groups, with significant differences between the two groups.ConclusionAll three interventions appeared to have alleviating effect on smartphone addiction. After 4 weeks, participants showed improved executive control and decision-making abilities. Specifically, significant effects were observed in the tDCS group, with increased P300 amplitudes in the frontal, parietal, and central regions, as well as FRN amplitudes in the central and frontal regions. This suggested that tDCS enhanced psychological resources and improved inhibition control capabilities.
Professional values, ethical climate and job satisfaction of nurses and their selected sociodemographic and occupational characteristics
Authors: Ozdoba Patrycja , Jurek Krzysztof , Dobrowolska Beata 
Published: November 2024

AimTo explore the relationship of selected socio-demographic and occupational characteristics of nurses and their level of professional values, hospital ethical climate and job satisfaction.MethodsCross-sectional study was conducted among 388 Polish nurses from the spring of 2021 to winter of 2023, in the eastern part of Poland; and followed by Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. Four research tools were used to collect data together with questionnaire for socio-demographic and occupational characteristics.ResultsProfessional values such as activism correlate negatively with religious beliefs (Z = −1.789; p = 0.044), this means that nurses who are more involved in professional change activities are likely to be less associated with religious beliefs. A positive correlation was observed between the Ethical Hospital Climate Survey subscale—peer relations and nurses’ education level (H = 5.638; p = 0.048), indicating that a higher education level was associated with better relationships with colleagues at work. A negative relationship was identified between nurses’ external job satisfaction and their marital status (Z = −1.958; p = 0.040), that is, married nurses feel less satisfaction with the external aspects of their jobs than their single colleagues.DiscussionThese findings underscore that medical staff management should take into account both sociodemographic factors [e.g., age, education, place of residence, marital status, religious beliefs, as well as professional factors (working hours, qualification course, etc.)] that affect nurses’ professional values, job satisfaction and the ethical climate of the hospital. These underscore the need to adapt management strategies to the individual needs of employees, which can contribute to improving working conditions in healthcare facilities.Data collection toolThe data collection tool consists of four sections.DemographicsCollected background and demographic information.Nurses’ professional values scale (NPVS-3)Assessed professional values among nurses.Hospital ethical climate survey (HECS)Assessed hospital ethical climate among nurses.Minnesota satisfaction questionnaire-short form (MSQ-SF)Assessed job satisfaction levels among nurses.
Predicting patient reported outcome measures: a scoping review for the artificial intelligence-guided patient preference predictor
Authors: Balch Jeremy A. , Chatham A. Hayes , Hong Philip K. W. , Manganiello Lauren , Baskaran Naveen , Bihorac Azra , Shickel Benjamin , Moseley Ray E. , Loftus Tyler J. 
Published: November 2024

BackgroundThe algorithmic patient preference predictor (PPP) has been proposed to aid in decision making for incapacitated patients in the absence of advanced directives. Ethical and legal challenges aside, multiple practical barriers exist for building a personalized PPP. Here, we examine previous work using machine learning to predict patient reported outcome measures (PROMs) for capacitated patients undergoing diverse procedures, therapies, and life events. Demonstrating robust performance in predicting PROMs for capacitated patients could suggest opportunities for developing a model tailored to incapacitated ones.MethodsWe performed a scoping review of PubMed, Embase, and Scopus using the PRISMA-ScR guidelines to capture studies using machine learning to predict PROMs following a medical event alongside qualitative studies exploring a theoretical PPP.ResultsSixty-eight studies used machine learning to evaluate PROMs; an additional 20 studies focused on a theoretical PPP. For PROMs, orthopedic surgeries (n = 33) and spinal surgeries (n = 12) were the most common medical event. Studies used demographic (n = 30), pre-event PROMs (n = 52), comorbidities (n = 29), social determinants of health (n = 30), and intraoperative variables (n = 124) as predictors. Thirty-four different PROMs were used as the target outcome. Evaluation metrics varied by task, but performance was overall poor to moderate for the best reported scores. In models that used feature importance, pre-event PROMs were the most predictive of post-event PROMs. Fairness assessments were rare (n = 6). These findings reinforce the necessity of the integrating patient values and preferences, beyond demographic factors, to improve the development of personalized PPP models for incapacitated patients.ConclusionThe primary objective of a PPP is to estimate patient-reported quality of life following an intervention. Use of machine learning to predict PROMs for capacitated patients introduces challenges and opportunities for building a personalized PPP for incapacitated patients without advanced directives.
Innovating neurosurgical training: a comprehensive evaluation of a 3D-printed intraventricular neuroendoscopy simulator and systematic review of the literature
Authors: Saemann Attill , De Rosa Adriana , Zubizarreta Oteiza Jokin , Sharma Neha , Thieringer Florian M. , Soleman Jehuda , Guzman Raphael 
Published: November 2024

ObjectiveThe objective of this study was to develop and evaluate a low-cost 3D-printed simulator to improve the ability of neurosurgical residents to handle and coordinate endoscopes in performing technically demanding procedures such as neuroendoscopic removal of ventricular tumors or endoscopic third ventriculostomy (ETV).MethodsThe simulator was developed, printed in-house, and evaluated in a trial involving neurosurgery residents who performed ETV and intraventricular tumor resection tasks using it. Participants completed a questionnaire that assessed various aspects of the simulator's effectiveness, including anatomical visualization, procedural understanding, competency enhancement, and subjective impressions.ResultsA total of 12 participants were included in the evaluation. The majority (n = 7, 53.85%) were male, with a mean age of 29.8 ± 3.27 years and 4 ± 2 years of neurosurgical experience. All participants agreed or strongly agreed (4.5 ± 0.50) that the 3D printed simulator helped develop systematic intraventricular visualization and understanding of surgical steps (4.42 ± 0.64). The handling of the endoscope was rated as realistic (4.5 ± 0.50), while the haptic qualities of the tumor were rated lower (3.83 ± 0.80; 3.92 ± 0.64). Training increased competence (4.25 ± 0.45) and coordination skills (4.5 ± 0.50), with 75% (n = 9) feeling more confident with neuroendoscopic instruments and 91.7% (n = 11) in future procedures.ConclusionThe developed 3D-printed simulator offers an accessible and practical training resource for neurosurgical residents, addressing the limitations of traditional training methods. The simulator appears to improve procedural skills and the competence of future neurosurgeons, potentially improving patient safety and outcomes in neurosurgical practice.
Use of serum-free media for peripheral blood mononuclear cell culture and the impact on T and B cell readouts
Authors: Cochrane Stella , Saib Ouarda , Sheffield David 
Published: November 2024

IntroductionAs part of a wider programme of work developing next-generation risk assessment approaches (NGRA) using non-animal methods (NAMs) for safety assessment of materials, Unilever SEAC is exploring the use of a peripheral blood mononuclear cell (PBMC) system to investigate how cells from different arms of the human immune system are impacted by different treatments. To maximise human relevance, the cell cultures are supported by human serum, but this came with some challenges, including an inability to measure induced levels of immunoglobulins due to high background levels. Therefore, a study comparing use of human sera containing media with three different chemically defined serum-free media was undertaken.Materials and MethodsPBMC were isolated from healthy donors and cultured in the absence (media alone) or presence of stimulation reagents (CpG-ODN plus IL-15, Pokeweed Mitogen (PWM) or Cytostim (CS)), in RPMI plus human serum, AIM-V, CTS OpTmizer T cell expansion SFM or X-VIVO 15 media. T cell (CD4+ and CD8+) and B cell proliferation and viability were measured after 6 days, along with levels of total IgG in the cell culture supernatants.ResultsEach of the serum-free media tested supported good levels of viable and proliferating T cells and B cells over the 6 days of culture, with only a few, small differences across the media, when there was no stimulation. They also enabled detection of a stimulation-evoked increase in IgG levels. There were however some differences in the viability and proliferation responses of T and B cells, to different stimuli, across the different media.DiscussionThe serum-free media formulations tested in this study offer defined systems for. measuring B cell IgG responses, in vitro, in either a ‘T cell-independent’ (CpG + IL-15) or “T cell-dependent” (PWM or CS) manner and for assessing B cell proliferation, particularly in response to a “T cell-independent” stimulus. However, there are some characteristics and features endowed by human serum that appear to be missing. Therefore, further work is required to optimise animal-free, chemically defined culture conditions for PBMC based assays for inclusion in tiered safety assessments.