bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glaucoma Features in an East African Population: A 6-Year Cohort Study of Older Adults in Nakuru, Kenya
Authors: Bastawrous Andrew , Mathenge Wanjiku , Buchan John , Kyari Fatima , Peto Tunde , Rono Hillary , Weiss Helen A. , Macleod David , Foster Allen , Burton Matthew J. , Kuper Hannah 
Published: March 2018

Purpose:
Glaucoma is a leading cause of blindness in people of African descent. Minimal data is available from African population–based cohort studies. The primary aims of this study were to describe the normative distribution of glaucoma features to enable glaucoma classification and to assess risk factors for those with glaucoma at follow-up among people aged 50 years and above in Kenya.

Materials and Methods:
Random cluster sampling with probability proportionate to size was used to select a representative cross-sectional sample of adults aged 50 years and above in 2007 to 2008 in Nakuru District, Kenya. A 6-year follow-up was undertaken in 2013 to 2014. Comprehensive ophthalmic examination included visual acuity, digital retinal photography, visual fields, intraocular pressure, optical coherence tomography, and independent grading of optic nerve images. We report glaucoma features, prevalence and predictors for glaucoma based on the International Society for Geographical & Epidemiological Ophthalmology (ISGEO) criteria. Measures were estimated using a Poisson regression model and including inverse-probability weighting for loss to follow-up.

Results:
At baseline, 4414 participants aged 50 years and above underwent examination. Anterior chamber optical coherence tomography findings: mean anterior chamber angle of 36.6 degrees, mean central corneal thickness of 508.1 μm and a mean anterior chamber depth of 2.67 mm. A total of 2171 participants were examined at follow-up. The vertical cup to disc ratio distribution was 0.7 and 0.8 at the 97.5th and 99.5th percentiles, respectively. A total of 88 (4.3%, 95% confidence interval, 3.5%-5.9%) of participants at follow-up had glaucoma consistent with ISGEO criteria. A relative afferent pupillary defect and raised intraocular pressure were associated with the diagnosis.

Conclusions:
Glaucoma is a public health challenge in low-resource settings. Research into testing and treatment modalities in Africa is needed.
Altered connectivity of the right anterior insula drives the pain connectome changes in chronic knee osteoarthritis
Authors: Cottam William J. , Iwabuchi Sarina J. , Drabek Marianne M. , Reckziegel Diane , Auer Dorothee P. 
Published: March 2018

Abstract
Resting-state functional connectivity (FC) has proven a powerful approach to understand the neural underpinnings of chronic pain, reporting altered connectivity in 3 main networks: the default mode network (DMN), central executive network, and the salience network (SN). The interrelation and possible mechanisms of these changes are less well understood in chronic pain. Based on emerging evidence of its role to drive switches between network states, the right anterior insula (rAI, an SN hub) may play a dominant role in network connectivity changes underpinning chronic pain. To test this hypothesis, we used seed-based resting-state FC analysis including dynamic and effective connectivity metrics in 25 people with chronic osteoarthritis (OA) pain and 19 matched healthy volunteers. Compared with controls, participants with painful knee OA presented with increased anticorrelation between the rAI (SN) and DMN regions. Also, the left dorsal prefrontal cortex (central executive network hub) showed more negative FC with the right temporal gyrus. Granger causality analysis revealed increased negative influence of the rAI on the posterior cingulate (DMN) in patients with OA in line with the observed enhanced anticorrelation. Moreover, dynamic FC was lower in the DMN of patients and thus more similar to temporal dynamics of the SN. Together, these findings evidence a widespread network disruption in patients with persistent OA pain and point toward a driving role of the rAI.
Gray matter correlates of pressure pain thresholds and self-rated pain sensitivity: a voxel-based morphometry study
Authors: Ruscheweyh Ruth , Wersching Heike , Kugel Harald , Sundermann Benedikt , Teuber Anja 
Published: March 2018

Abstract
Individual differences in sensitivity to pain are large and have clinical and scientific importance. Although heavily influenced by situational factors, they also relate to genetic factors and psychological traits, and are reflected by differences in functional activation in pain-related brain regions. Here, we used voxel-based morphometry to investigate if individual pain sensitivity is related to local gray matter volumes. Pain sensitivity was determined using (1) index finger pressure pain thresholds (PPTs) and (2) pain intensity ratings of imagined painful situations as assessed by the Pain Sensitivity Questionnaire (PSQ) in 501 population-based subjects participating in the BiDirect Study. Pain Sensitivity Questionnaire scores were positively associated with gray matter in 2 symmetrical clusters, with a focus on the parahippocampal gyrus, extending to the hippocampus, fusiform gyrus, BA19, putamen, and insula (P < 0.05 corrected), but the effect was small (R
2 = 0.045-0.039). No negative associations with the PSQ and no associations with the PPT reached significance. Parahippocampal activation during pain and altered parahippocampal gray matter in chronic pain have been reported, which would be consistent with positive associations with PSQ scores. Alternatively, associations of PSQ scores with the parahippocampal and fusiform gray matter could relate to the visual imagination of painful situations required by the PSQ, not to pain sensitivity itself. Regarding PPTs, the present data obtained in a large sample strongly suggest an absence of associations of this parameter with gray matter volume. In conclusion, the present results argue against a strong association between pain sensitivity and local gray matter volumes.
Epidermis and Enamel
Authors: Barsley Robert E. , Bernstein Mark L. , Brumit Paula C. , Dorion Robert B.J. , Golden Gregory S. , Lewis James M. , McDowell John D. , Metcalf Roger D. , Senn David R. , Sweet David , Weems Richard A. 
Published: March 2018

AbstractCritics describe forensic dentists' management of bitemark evidence as junk science with poor sensitivity and specificity and state that linkages to a biter are unfounded. Those vocal critics, supported by certain media, characterize odontologists' previous errors as egregious and petition government agencies to render bitemark evidence inadmissible. Odontologists acknowledge that some practitioners have made past mistakes. However, it does not logically follow that the errors of a few identify a systemic failure of bitemark analysis. Scrutiny of the contentious cases shows that most occurred 20 to 40 years ago. Since then, research has been ongoing and more conservative guidelines, standards, and terminology have been adopted so that past errors are no longer reflective of current safeguards. The authors recommend a comprehensive root analysis of problem cases to be used to determine all the factors that contributed to those previous problems. The legal community also shares responsibility for some of the past erroneous convictions. Currently, most proffered bitemark cases referred to odontologists do not reach courts because those forensic dentists dismiss them as unacceptable or insufficient for analysis. Most bitemark evidence cases have been properly managed by odontologists. Bitemark evidence and testimony remain relevant and have made significant contributions in the justice system.
Cost and Cost-Effectiveness of a Demand Creation Intervention to Increase Uptake of Voluntary Medical Male Circumcision in Tanzania: Spending More to Spend Less
Authors: Torres-Rueda Sergio , Wambura Mwita , Weiss Helen A. , Plotkin Marya , Kripke Katharine , Chilongani Joseph , Mahler Hally , Kuringe Evodius , Makokha Maende , Hellar Augustino , Schutte Carl , Kazaura Kokuhumbya J. , Simbeye Daimon , Mshana Gerry , Larke Natasha , Lija Gissenge , Changalucha John , Vassall Anna , Hayes Richard , Grund Jonathan M. , Terris-Prestholt Fern 
Published: March 2018

Background:
Although voluntary medical male circumcision (VMMC) reduces the risk of HIV acquisition, demand for services is lower among men in most at-risk age groups (ages 20–34 years). A randomized controlled trial was conducted to assess the effectiveness of locally-tailored demand creation activities (including mass media, community mobilization, and targeted service delivery) in increasing uptake of campaign-delivered VMMC among men aged 20–34 years. We conducted an economic evaluation to understand the intervention's cost and cost-effectiveness.

Setting:
Tanzania (Njombe and Tabora regions).

Methods:
Cost data were collected on surgery, demand creation activities, and monitoring and supervision related to VMMC implementation across clusters in both trial arms, as well as start-up activities for the intervention arms. The Decision Makers' Program Planning Tool was used to estimate the number of HIV infections averted and related cost savings, given the total VMMCs per cluster. Disability-adjusted life years were calculated and used to estimate incremental cost-effectiveness ratios.

Results:
Client load was higher in the intervention arms than in the control arms: 4394 vs. 2901 in Tabora and 1797 vs. 1025 in Njombe, respectively. Despite additional costs of tailored demand creation, demand increased more than proportionally: mean costs per VMMC in the intervention arms were $62 in Tabora and $130 in Njombe, and in the control arms $70 and $191, respectively. More infections were averted in the intervention arm than in the control arm in Tabora (123 vs. 67, respectively) and in Njombe (164 vs. 102, respectively). The intervention dominated the control because it was both less costly and more effective. Cost savings were observed in both regions stemming from the antiretroviral treatment costs averted as a result of the VMMCs performed.

Conclusions:
Spending more to address local preferences as a way to increase uptake of VMMC can be cost-saving.
99mTc MDP SPECT-CT–Based Modified Mirels Classification for Evaluation of Risk of Fracture in Skeletal Metastasis
Authors: Riaz Saima , Bashir Humayun , Niazi Imran Khalid , Butt Sumera , Qamar Faisal 
Published: March 2018

Abstract
Mirels’ scoring system quantifies the risk of sustaining a pathologic fracture in osseous metastases of weight bearing long bones. Conventional Mirels’ scoring is based on radiographs. Our pilot study proposes 99mTc MDP bone SPECT-CT based modified Mirels’ scoring system and its comparison with conventional Mirels’ scoring. Cortical lysis was noted in 8(24%) by SPECT-CT versus 2 (6.3%) on X-rays. Additional SPECT-CT parameters were; circumferential involvement [1/4 (31%), 1/2 (3%), 3/4 (37.5%), 4/4 (28%)] and extra-osseous soft tissue [3%]. Our pilot study suggests the potential role of SPECT-CT in predicting risk of fracture in osseous metastases.
Adrenal Hemangioma Definite Diagnosis on CT, MRI, and FDG PET in a Patient With Primary Lung Cancer
Authors: Wilson Benjamin , Becker Allison , Estes Thomas , Keshavamurthy Jayanth , Pucar Darko 
Published: March 2018

Abstract
Distinguishing adrenal incidentalomas (5% of all abdominal CT scans) from metastasis is a frequent diagnostic challenge in primary malignancies with a propensity for adrenal spread, such as lung cancer. Adrenal myelolipoma and adenoma can be definitively characterized as benign by demonstrating gross and microscopic fat, respectively, on CT and MRI and an absence of abnormal uptake on PET. Unfortunately, adrenal sampling is frequently necessary in indeterminate cases for adequate staging of extra-adrenal primary malignancy. We present a lung cancer case in which definite characterization of a rare adrenal hemangioma with CT, PET, and MRI avoided unnecessary adrenal sampling.
Characterisation of carried and invasive Neisseria meningitidis isolates in Shanghai, China from 1950 to 2016: implications for serogroup B vaccine implementation
Authors: Chen Mingliang , Rodrigues Charlene M.C. , Harrison Odile B , Zhang Chi , Tan Tian , Chen Jian , Zhang Xi , Chen Min , Maiden Martin C.J. 
Published: March 2018

AbstractBackgroundSerogroup B invasive meningococcal disease (IMD) is increasing in China, little is known however, about these meningococci. This study characterises a collection of isolates associated with IMD and carriage in Shanghai and assesses current vaccine strategies.MethodsIMD epidemiological data in Shanghai from 1950–2016 were obtained from the National Notifiable Diseases Registry System, with 460 isolates collected for analysis including, 169 from IMD and 291 from carriage. Serogroup B meningococcal (MenB) vaccine coverage was evaluated using Bexsero® Antigen Sequence Type (BAST).ResultsSeven IMD epidemic periods have been observed in Shanghai since 1950, with incidence peaking from February to April. Analyses were divided according to the period of meningococcal polysaccharide vaccine (MPV) introduction: (i) pre-MPV-A, 1965-1980; (ii) post-MPV-A, 1981-2008; and (iii) post-MPV-A+C, 2009-2016. IMD incidence decreased from 55.4/100,000 to 0.71 then to 0.02, and corresponded with shifts from serogroup A ST-5 complex (MenA:cc5) to MenC:cc4821 then MenB:cc4821. MenB IMD became predominant (63.2%) in the post-MPV-A+C period, of which 50% were caused by cc4821, with the highest incidence in infants (0.45/100,000) and a case-fatality rate of 9.5%. IMD was positively correlated with carriage rates. Data indicate that fewer than 25% of MenB isolates in the post-MPV-A+C period may be covered by the vaccines Bexsero®, Trumenba®, or a PorA-based vaccine, NonaMen.ConclusionsA unique IMD epidemiology is found in China, changing periodically from hyperepidemic to low-level endemic disease. MenB IMD now dominates in Shanghai, with isolates harbouring diverse antigenic variants potentially beyond coverage with licenced OMV- and protein-based MenB vaccines.SummaryMeningococcal disease in Shanghai, China is described and current vaccine approaches evaluated. Since 1950, MenA:cc5 shifted to MenC:cc4821 then MenB:cc4821, with MenB dominating since 2009. Distinct antigens potentially beyond coverage with licensed OMV- and protein-based MenB vaccines were found.
FUN-LOV: Fungal LOV domains for optogenetic control of gene expression and flocculation in yeast
Authors: Salinas Francisco , Rojas Vicente , Delgado Verónica , López Javiera , Agosin Eduardo , Larrondo Luis F. 
Published: March 2018

AbstractOptogenetic switches permit accurate control of gene expression upon light stimulation. These synthetic switches have become a powerful tool for gene regulation, allowing modulation of customized phenotypes, overcoming the obstacles of chemical inducers and replacing their use by an inexpensive resource: light. In this work, we implemented FUN-LOV; an optogenetic switch based on the photon-regulated interaction of WC-1 and VVD, two LOV (Light Oxygen Voltage) blue-light photoreceptors from the fungus Neurospora crassa. When tested in yeast, FUN-LOV yields light-controlled gene expression with exquisite temporal resolution, and a broad dynamic range of over 1300-fold, as measured by a luciferase reporter. We also tested the FUN-LOV switch for heterologous protein expression in Saccharomyces cerevisiae, where Western blot analysis confirmed strong induction upon light stimulation, surpassing by 2.5 times the levels achieved with a classic GAL4/galactose chemical inducible system. Additionally, we utilized FUN-LOV to control the ability of yeast cells to flocculate. Light-controlled expression of the flocculin encoding gene FLO1, by the FUN-LOV switch, yielded Flocculation in Light (FIL), whereas the light-controlled expression of the co-repressor TUP1 provided Flocculation in Darkness (FID). Overall, the results revealed the potential of the FUN-LOV optogenetic switch to control two biotechnologically relevant phenotypes such as heterologous protein expression and flocculation, paving the road for the engineering of new yeast strains for industrial applications. Importantly, FUN-LOV’ s ability to accurately manipulate gene expression, with a high-temporal dynamic range, can be exploited in the analysis of diverse biological processes in various organisms.ImportanceOptogenetic switches are molecular devices which allow the control of different cellular processes by light, such as gene expression, providing a versatile alternative to chemical inducers. Herein, we report a novel optogenetic switch (FUN-LOV) based on the LOV-domain interaction of two blue-light photoreceptors (WC-1 and VVD) from the fungus N. crassa. In yeast cells, FUN-LOV allowed tight regulation of gene expression, with low background in darkness and a highly dynamic and potent control by light. We used FUN-LOV to optogenetically manipulate, in yeast, two biotechnologically relevant phenotypes: heterologous protein expression and flocculation, resulting in strains with potential industrial applications. Importantly, FUN-LOV can be implemented in diverse biological platforms to orthogonally control a multitude of cellular processes.
Inexpensive, scalable camera system for tracking rats in large spaces
Authors: Saxena Rajat , Barde Warsha , Deshmukh Sachin S. 
Published: March 2018

AbstractMost studies focused on understanding the neural circuits underlying spatial navigation are restricted to small behavioral arenas (≤ 1 m2) because of the limits imposed by the cables extending from the animal to the recording system. New wireless recording systems have significantly increased the recording range. However, the size of arena is still constrained by the lack of a video tracking system capable of monitoring the animal’s movements over large areas integrated with these recording systems. We developed and benchmarked a novel, open-source, scalable multi-camera tracking system based on commercially available and low-cost hardware (Raspberry Pi computers and Raspberry Pi cameras). This Picamera system was used in combination with a wireless recording system for characterizing neural correlates of space in environments of various sizes up to 16.5 m2. Spatial rate maps generated using the Picamera system showed improved accuracy in estimating spatial firing characteristics of neurons compared to a popular commercial system, due to its better temporal accuracy. The system also showed improved accuracy in estimating head direction cell tuning as well as theta phase precession in place cells. This improved temporal accuracy is crucial for accurately aligning videos from multiple cameras in large spaces and characterizing spatially modulated cells in a large environment.
Laminar signal extraction over extended cortical areas by means of a spatial GLM
Authors: van Mourik Tim , van der Eerden Jan PJM , Bazin Pierre-Louis , Norris David G 
Published: March 2018

AbstractThere is converging evidence that distinct neuronal processes leave distinguishable footprints in the laminar BOLD response. However, even though the achievable spatial resolution in functional MRI has much improved over the years, it is still challenging to separate signals arising from different cortical layers. In this work, we propose a new method to extract laminar signals. We use a spatial General Linear Model in combination with the equivolume principle of cortical layers to unmix laminar signals instead of interpolating through and integrating over a cortical area: thus reducing partial volume effects. Not only do we provide a mathematical framework for extracting laminar signals with a spatial GLM, we also illustrate that the best case scenarios of existing methods can be seen as special cases within the same framework. By means of simulation, we show that this approach has a sharper point spread function, providing better signal localisation. We further assess the partial volume contamination in cortical profiles from high resolution human ex vivo and in vivo structural data, and provide a full account of the benefits and potential caveats. We eschew here any attempt to validate the spatial GLM on the basis of fMRI data as a generally accepted ground-truth pattern of laminar activation does not currently exist. This approach is flexible in terms of the number of layers and their respective thickness, and naturally integrates spatial regularisation along the cortex, while preserving laminar specificity. Care must be taken, however, as this procedure of unmixing is susceptible to sources of noise in the data or inaccuracies in the laminar segmentation.
Comparing the web and mobile platforms of a social Q&A service from the user’s perspective
Authors: Chen Xiaoyu , Chua Alton Y.K. , Deng Shengli 
Published: March 2018

Purpose
As an increasing number of users have acquired information across the web and mobile platforms for social question and answering (Q&A), it is of interest to explore whether there are differences in social Q&A usages between the two platforms. The purpose of this paper is to compare web and mobile platforms of a social Q&A service from the user’s perspective in terms of three dimensions, namely, demographics, individual-based constructs, and information-based constructs.

Design/methodology/approach
Because Zhihu.com is one of the most popular social Q&A sites in China, the authors used online questionnaires to investigate its users’ perceptions of these three dimensions. From January to March 2016, the authors obtained 278 valid responses in total through snowball and convenient sampling. Collected data are analyzed through descriptive statistics and inferential statistics.

Findings
The results indicate that there exist significant differences between web users and mobile users on Zhihu.com in terms of gender, affinity, and information seeking. More specifically, compared to the male users, more female users rely on the mobile platform to access the information service; mobile users perceive higher affinity with Zhihu.com than web users; and mobile users perceive higher information-seeking intention than web users do.

Originality/value
Regarding the theoretical aspect, this study proposes a conceptual framework for comparison between the web and mobile platforms of social Q&A from the user’s perspective. Regarding the practical aspect, the comparative results of this study could give social Q&A service providers useful information about users’ differences between web and mobile platforms of social Q&A services.
Inclusion and work: addressing the global challenges for youth employment
Authors: Dhakal Subas P. , Connell Julia , Burgess John 
Published: March 2018

Purpose
The purpose of this paper is to outline the key global challenges relating to youth employment and consider some ways that they may be addressed to allow their inclusion in the contemporary workplace. Also, the paper provides a brief introduction and rationale for the other five articles comprising this special issue volume.

Design/methodology/approach
The approach concerns a review of the relevant literature and reports on the topic.

Findings
The challenges outlined in this paper and the others in this special issue volume emphasise the need for much more work to be done to address the current global challenges relating to youth unemployment. It points to: the difficulties many young workers face in taking the first step towards gaining employment; the need for stakeholder collaboration towards future policy investment as well as strategy implementation and integration.

Originality/value
To date, much of the research that has been conducted on the challenges of youth employment and inclusion appears to have focussed on Europe and the USA. This special issue volume includes countries that have been less researched to date: Australia, Indonesia, Malaysia, Mauritius, Singapore and the United Arab Emirates, thus adding to current understanding of the topic in those contexts.