bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reconfigurable Bioinspired Framework Nucleic Acid Nanoplatform Dynamically Manipulated in Living Cells for Subcellular Imaging
Authors: Peng Pai , Du Yi , Zheng Jiao , Wang Huihui , Li Tao 
Published: December 2018

AbstractIn nature, the formation of spider silk fibers begins with dimerizing the pH‐sensitive N‐terminal domains of silk proteins (spidroins) upon lowering pH, and provides a natural masterpiece for programmable assembly. Inspired by the similarity of pH‐dependent dimerization behaviors, introduced here is an i‐motif‐guided model to mimic the initial step of spidroin assembly at the subcellular level. A framework nucleic acid (FNA) nanoplatform is designed using two tetrahedral DNA nanostructures (TDNs) with different branched vertexes carrying a bimolecular i‐motif and a split ATP aptamer. Once TDNs enter acidic lysosomes within living cells, they assemble into a heterodimeric architecture, thereby enabling the formation of a larger‐size framework and meanwhile subcellular imaging in response to endogenous ATP, which can be dynamically manipulated by adjusting intracellular pH and ATP levels with external drug stimuli.
Titanium Carbene Complexes Stabilized by Alkali Metal Amides
Authors: Osseili Hassan , Truong Khai‐Nghi , Spaniol Thomas P. , Maron Laurent , Englert Ulli , Okuda Jun 
Published: December 2018

AbstractFacile α‐H elimination from tetrakis(trimethylsilylmethyl)titanium precursors to give adducts of (alkylidene)bis(alkyl)titanium complexes is induced by light alkali metal amides of the NNNN‐type macrocyclic anionic ligand Me3TACD [(Me3TACD)H=1,4,7‐trimethyl‐1,4,7,10‐tetraazacyclododecane]. In the crystal, the alkali metal interacts with the carbene carbon atom or with the CH2 group of the trimethylsilymethyl ligand. The nucleophilic character of the carbene carbon atom was shown by the reaction with benzophenone and terminal acetylenes.
Cross‐cultural consumer psychology
Authors: Shavitt Sharon , Barnes Aaron J. 
Published: December 2018

AbstractOver the last 25 years, cross‐cultural consumer psychology has developed into a well‐researched domain, delivering important insights into how cultural differences influence consumer phenomena. This review synthesizes critical developments in the last decade of research related to the most commonly studied cultural dimensions. First, we describe how cultural differences in individualism and collectivism offer new insights on consumer goal pursuit and self‐regulatory processes. Next, we highlight the role of holistic and analytic thinking in consumer phenomena ranging from pricing to branding. We then describe the horizontal and vertical refinement of individualism and collectivism, and the power distance belief dimension, both of which address cultural orientations toward hierarchy and power. As we describe, these distinctions have implications for important consumer outcomes such as impulse consumption and prosocial behavior. Finally, we look ahead to an emerging cultural distinction, normative tightness–looseness, and bring attention to significant shifts in the practice of cross‐cultural consumer research.
Techno‐economic analysis of jet‐fuel production from biorefinery waste lignin
Authors: Shen Rongchun , Tao Ling , Yang Bin 
Published: December 2018

AbstractUtilizing lignin feedstock along with cellulosic ethanol for the production of high‐energy‐density jet fuel offers a significant opportunity to enhance the overall operation efficiency, carbon conversion efficiency, economic viability, and sustainability of biofuel and chemical production. A patented catalytic process to produce lignin‐substructure‐based hydrocarbons in the jet‐fuel range from lignin was developed. Comprehensive techno‐economic analysis of this process was conducted through process simulation in this study. The discounted cash flow rate of return (DCFROR) method was used to evaluate a 2000 dry metric ton/day lignocellulosic ethanol biorefinery with the co‐production of lignin jet fuel. The minimum selling price of lignin jet fuel at a 10% discount rate was estimated to be in the range of $6.35–$1.76/gal depending on the lignin and conversion rate and capacity. With a production capacity of 1.5–16.6 million gallon jet fuel per year, capital costs ranged from $38.0 to $39.4 million. On the whole, the co‐production of jet fuel from lignin improved the overall economic viability of an integrated biorefinery process for corn ethanol production by raising co‐product revenue from jet fuels. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd
A Biomass‐Derived Carbon‐Based Electrocatalyst for Efficient N2 Fixation to NH3 under Ambient Conditions
Authors: Huang Hong , Xia Li , Cao RongRong , Niu Zhiguo , Chen Hongyu , Liu Qian , Li Tingshuai , Shi Xifeng , Asiri Abdullah M. , Sun Xuping 
Published: December 2018

AbstractCurrently, NH3 production primarily depends on the Haber–Bosch process, which operates at elevated temperatures and pressures and leads to serious CO2 emissions. Electrocatalytic N2 reduction offers an environmentally benign approach for the sustainable synthesis of NH3 under ambient conditions. This work reports the development of biomass‐derived amorphous oxygen‐doped carbon nanosheet (O−CN) using tannin as the precursor. As a metal‐free electrocatalyst for N2‐to‐NH3 conversion, such O−CN shows high catalytic performances, achieving a large NH3 yield of 20.15 μg h−1 mg−1cat. and a high Faradic efficiency of 4.97 % at −0.6 V vs. reversible hydrogen electrode (RHE) in 0.1 m HCl at ambient conditions. Remarkably, it also exhibits high electrochemical selectivity and durability.
Elektrohydraulische Zerkleinerung von carbonfaserverstärkten Kunststoffen für das Carbonfaser‐Recycling
Authors: Orzol Carsten H. M. , Lieberwirth Holger 
Published: December 2018

AbstractBasierend auf der Auswertung von Untersuchungen der mittleren Faserlänge, des massespezifischen Energieaufwands, E‐Moduls und Mindestrestmatrixgehalts kann die Gewinnung von freigelegten Carbonfasern aus carbonfaserverstärkten Kunststoffen mittels elektrohydraulischer Zerkleinerung als Downcycling betrachtet werden. Das bedeutet, der erzielte Sekundärrohstoff weist eine geringere Produktqualität als die Neuware auf und seine Herstellung ist mit höherem Energieaufwand als die Herstellung der Neuware verbunden.
Insect herbivory on seedlings of rainforest trees: Effects of density and distance of conspecific and heterospecific neighbors
Authors: Downey Harriet , Lewis Owen T. , Bonsall Michael B. , Fernandez D. Catalina , Gripenberg Sofia 
Published: December 2018

AbstractNatural enemies of plants such as insect herbivores can contribute to structuring and maintaining plant diversity in tropical forests. Most research in this area has focused on the role of specialized enemies and the extent to which herbivory on individual plant species is density‐dependent. Relatively few insect herbivores specialize on a single host plant species. Insect herbivores that feed on more than one plant species may link the regeneration dynamics of their host species through “apparent competition” or “apparent mutualism.” We investigated herbivory and survival of seedlings of two tropical tree species (Cordia alliodoraandCordia bicolor) in the forests of Barro Colorado Island (Panama). We used experiments and observations to assess seedling fate in relation to the presence of conspecifics and heterospecifics across a range of spatial scales. Herbivory significantly increased seedling mortality and was highest at high local densities ofC. alliodoraseedlings. There was also evidence that high local densities ofC. alliodoraincreased herbivory on co‐occurringC. bicolorseedlings.Synthesis.The elevated rates of seedling herbivory at high densities of conspecifics documented in our study are consistent with the predictions of the Janzen–Connell hypothesis, which explains how so many plant species can coexist in tropical forests. Our data also highlight the possibility that herbivore‐mediated density‐dependence, facilitated by herbivores that feed on multiple plant species, can also occuracrossplant species. Enemy‐mediated indirect effects of this sort have the potential to structure plant communities.
Electrochemical Determination of Sunscreens Agents in Cosmetic Using Square Wave Voltammetry
Authors: Lopes Neves Raiane Aparecida , Moreira Araujo Fausto , Siqueira Pacheco Felipe , Chevitarese Azevedo Gustavo , Costa Matos Maria Auxiliadora , Camargo Matos Renato 
Published: December 2018

AbstractSunscreen agents and in particular UV filters are compounds added in different cosmetic formulations, that has the function of preventing damage caused by sun exposition. Therefore, this paper proposes the development of a simple, fast and reliable electroanalytical method utilizing square wave voltammetry (SWV) to the determination of Benzophenone‐3 (BZ‐3), camphor 4‐methylbenzylidene (MBC) and 2‐ethylhexyl‐4‐methoxycinnamate (OMC) in cosmetic samples. The electrochemical system consisted of a cell with three electrodes: work – gold electrode modified, reference – Ag/AgCl(sat) and auxiliary – platinum, using as supporting electrolyte 4.0 mL of Britton Robinson Buffer 0.04 mol L−1 (pH=4.0), 1.0 mL of methanol and 5.50×10−4 mol L−1 of cetyltrimethylammonium bromide (CTAB). The method was validated using three commercial sunscreen samples and the results showed recovery values between 83 and 98 %. The average values found for the analysed samples were 3.49 % m/m (728 mg L−1) to BZ‐3, 0.56 % m/m (113 mg L−1) to MBC and 0.99 % m/m (208 mg L−1) to OMC. The detection (DL) and quantification (QL) limits were 0.47 mg L−1 and 1.56 mg L−1 to BZ‐3, 0.77 mg L−1 and 2.58 mg L−1 to MBC and 0.78 mg L−1 and 2.59 mg L−1 to OMC, respectively. The sunscreen protector samples were also evaluated by high‐performance liquid chromatography (HPLC) demonstrating a good correlation between the results and compared the results with allowed concentration.
Perspective of Water Distribution Based on the Performance of Hydraulic Structures in the Varamin Irrigation Scheme (Iran)
Authors: Mohammadi Amir , Parvaresh Rizi Atefeh , Abbasi Nader 
Published: December 2018

AbstractDespite the extensive application of check and offtake structures in irrigation canals, their periodic assessment is generally not included in water management plans, especially in Iran. This issue would be more important when there are many problems in water distribution in irrigation projects. In this study the performance and operating status of different hydraulic structures in the Varamin irrigation scheme were evaluated using a precise flowmeter. Results show that Neyrpic modules have an error in range of −8 to 83% and these structures, on average, delivered 22% more water. The discharge coefficient of duckbill weirs has changed considerably as well and they are not able to regulate the water level appropriately, because of ageing and physical destruction. The results showed that to achieve an acceptable performance of Neyrpic modules in this scheme it is essential to perform some physical modifications, such as cleaning and sealing the gates, removing sediment around the gates, replacing old gates and continuous inspection of infrastructure. A series of measurements to estimate water losses during the conveyance process show that the conveyance efficiency for each 1000 m length of main, secondary and tertiary canals are respectively 95.0, 91.5 and 89.3%. Consequently to achieve a suitable water distribution pattern, preventive maintenance should be considered. © 2018 John Wiley & Sons, Ltd.
Thermo‐sensitive nanogel‐laden bicontinuous microemulsion drug‐eluting contact lenses
Authors: Lee Se‐Hee , Kim Ho‐Joong , Kim Duck‐Hyun , Chang Won‐Seok , Vales Temmy Pegarro , Kim Jae‐Woo , Kim Ki‐Hong , Kim Jong‐Ki 
Published: December 2018

AbstractThe bicontinuous microemulsion contact lens (BMCL) has nanoporous biphasic structures (100–250 nm) that are interconnected via multiple nano‐channels, providing suitable retention of various drugs for glaucoma. Timolol maleate (TM)‐carried thermosensitive poly(N‐isopropylacrylamide) (PNIPAM) nanogel (30–50 nm) was incorporated into BMCLs by soaking or by centrifuging plus soaking. Here, we present drug‐loading and release in silicon‐ or polyethylene oxide‐microemulsion BMCLs under various conditions. Nanoporous BMCLs containing thermosensitive TM‐laden nanogel were capable of potent body‐temperature‐triggered release of TM. Daily drug release was controllable according to the initial volume of drug‐loaded (VDL) and loading method for sustained drug release, making them reduce drug‐loss during transportation or storage. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1159–1169, 2019.
Reference Ranges for Trabecular Bone Score in Australian Men and Women: A Cross‐Sectional Study
Authors: Anderson Kara B , Holloway‐Kew Kara L , Hans Didier , Kotowicz Mark A , Hyde Natalie K , Pasco Julie A 
Published: December 2018

ABSTRACTTrabecular bone score (TBS) is a novel method for indirectly assessing trabecular microarchitecture at the lumbar spine, providing information complementary to areal BMD. However, limited reference ranges exist for the normative distribution of TBS, particularly in men. The aim of this study was to develop such a reference range in Australian men and women. This study included 894 men and 682 women (aged 24 to 98 years) enrolled in the Geelong Osteoporosis Study. TBS was determined retrospectively by analysis of lumbar spine DXA scans (Lunar Prodigy) using TBS iNsight software (version 2.2). Multivariable regression techniques were used to determine best‐fit models for TBS incorporating age, height, and weight. Age‐related differences in TBS were best modelled with a linear relationship in men and a cubic relationship in women. Combined best‐fit models for TBS included age and weight in men, and age and height in women. This study provides normative reference ranges for TBS in Australian men and women, and further indicates that TBS may identify individuals at risk for fracture despite normal BMD. © 2018 The Authors. JBMR Plus Published by Wiley Periodicals, Inc. on behalf of the American Society for Bone and Mineral Research.
Upregulation of circular RNA circ_0000502 predicts unfavorable prognosis in osteosarcoma and facilitates cell progression via sponging miR‐1238
Authors: Qi Hongfei , Sun Yue , Jiang Yuehong , Li Xiaolin 
Published: December 2018

AbstractGrowing evidence suggests that circular RNAs (circRNAs) play a significant role in regulating cancer initiation and metastasis. Osteosarcoma (OS) is a sophisticated disease with various genes activated or silenced. In this study, we defined a novel cancer‐related circRNA, circ_0000502 in OS progression. qRT‐PCR was conducted to detect its expression level in OS tissue samples and cell lines. In addition, the clinical significance of circ_0000502 was investigated. Afterwards, gain‐of‐function and loss‐of‐function in vitro assays were performed to detect the cell growth, apoptosis, migration, and invasion altered by circ_0000502 by CCK‐8, clone‐forming, flow cytometry, and transwell experiments. Xenograft study was performed to validate the in vitro data. The luciferase reporter assay was used to explore the mechanism of circ_0000502. Circ_0000502 was identified upregulated in both OS tissue specimens and cells. In addition, its expression predicts clinical severity and unfavorable prognosis in the 63 recruited patients with OS. Circ_0000502 facilitated cell proliferation, migration, and invasion in OS cells and inhibited cell apoptosis. The animal study further confirmed the in vitro results. For mechanism exploration, circ_0000502 could directly sponge microRNA (miR)‐1238, and the oncogenic functions of circ_0000502 is partially dependent on its regulation of miR‐1238 proved by rescue assays. In summary, this study might help to develop rational predictive and therapeutic target for patients with OS.
AGAP2‐AS1 serves as an oncogenic lncRNA and prognostic biomarker in glioblastoma multiforme
Authors: Tian Yanlong , Zheng Yong , Dong Xin 
Published: December 2018

AbstractLong noncoding RNA (lncRNA) AGAP2 antisense RNA 1 (AGAP2‐AS1) has been suggested to function as an oncogenic lncRNA in lung cancer, breast cancer, and anaplastic glioma. However, the expression pattern and molecular mechanism of AGAP2‐AS1 in glioblastoma multiforme (GBM) remains unknown. The purpose of this study is to present more evidence about the clinical and biological function of AGAP2‐AS1 in GBM. In our results, we found AGAP2‐AS1 expression was increased in GBM compared with adjacent normal brain tissues or low‐grade glioma tissues, and there was no significantly different between low‐grade glioma tissues and normal tissues. Kaplan‐Meier survival analysis indicated patients with GBM having high‐expression of AGAP2‐AS1 had shorter overall survival time than those with low expression of AGAP2‐AS1. The loss‐of‐function studies showed that downregulation of AGAP2‐AS1 depressed cell proliferation, migration, and invasion, and promoted cell apoptosis in GBM. In summary, AGAP2‐AS1 is a prognostic biomarker for patients with GBM, and functions as an oncogenic lncRNA to modulate GBM cell proliferation, apoptosis, migration, and invasion, which suggests that AGAP2‐AS1 is potential therapeutic target for GBM.
CREB/Wnt10b mediates the effect of COX‐2 on promoting BMP9‐induced osteogenic differentiation via reducing adipogenic differentiation in mesenchymal stem cells
Authors: Liao Yun‐Peng , Du Wei‐Min , Hu Ying , Li Fu‐Shu , Ma Yan , Wang Han , Zhu Jia‐Hui , Zhou Ya , Li Qin , Su Yu‐Xi , He Bai‐Cheng 
Published: December 2018

AbstractBone morphogenetic protein 9 (BMP9) is one of the most potent osteogenic factors, which may be a potential candidate for bone tissue engineering. However, the osteogenic capacity of BMP9 still need to be further enhanced. In this study, we determined the effect of Wnt10b on BMP9‐induced osteogenic differentiation in mesenchymal stem cell (MSCs) and the possible mechanism underlying this process. We introduced the polymerase chain reaction (PCR), Western blot analysis, histochemical stain, ectopic bone formation, and microcomputed tomography analysis to evaluate the effect of Wnt10b on BMP9‐induced osteogenic differentiation. Meanwhile, PCR, Western blot analysis, chromatin immunoprecipitation, and immunoprecipitation were used to analyze the possible relationship between BMP9 and Wnt10b. We found that BMP9 upregulates Wnt10b in C3H10T1/2 cells. Wnt10b increases the osteogenic markers and bone formation induced by BMP9 in C3H10T1/2 cells, and silencing Wnt10b decreases these effects of BMP9. Meanwhile, Wnt10b enhances the level of phosphorylated Smad1/5/8 (p‐Smad1/5/8) induced by BMP9, which can be reduced by silencing Wnt10b. On the contrary, Wnt10b inhibits adipogenic markers induced by BMP9, which can be decreased by silencing Wnt10b. Further analysis indicated that BMP9 upregulates cyclooxygenase‐2 (COX‐2) and phosphorylation of cAMP‐responsive element binding (p‐CREB) simultaneously. COX‐2 potentiates the effect of BMP9 on increasing p‐CREB and Wnt10b, while silencing COX‐2 decreases these effects. p‐CREB interacts with p‐Smad1/5/8 to bind the promoter of Wnt10b in C3H10T1/2 cells. Our findings suggested that Wnt10b can promote BMP9‐induced osteogenic differentiation in MSCs, which may be mediated through enhancing BMP/Smad signal and reducing adipogenic differentiation; BMP9 may upregulate Wnt10b via the COX‐2/p‐CREB‐dependent manner.
Prognostic relevance of miR‐137 and its liver microenvironment regulatory target gene AFM in hepatocellular carcinoma
Authors: Wei Qian , Zhao Lin , Jiang Longyang , Bi Jia , Yu Zhaojin , Zhao Lan , Song Xinyue , Sun Mingli , Chen Yuzong , Wei Minjie 
Published: December 2018

AbstractMiR‐137 has been identified as potential hepatocellular carcinoma (HCC) prognostic biomarkers. Highly relevant HCC prognostic biomarkers may be derived from combinations of miR‐137 with its target genes involved in the regulation of liver microenvironment. This study aimed at the discovery of such a combination with improved HCC prognosis performance than miR‐137 or its target gene alone in a significantly higher number of HCC patients than previous studies. Analysis of the differentially expressed micro RNAs (miRNAs) between cancer and noncancer tissues reconfirmed miR‐137 to be among the most relevant prognostic miRNAs and the data of 375 HCC patients and 50 normal cases were from the Cancer Genome Atlas (TCGA) data sets. Target genes were identified by the established search methods and Kaplan–Meier survival analysis of HCC patients was used to evaluate the overall survival (OS) and recurrence‐free survival (RFS). Cox proportional hazards regression indicated that the miR‐137 and its target gene AFM combination is an independent prognostic factor for the OS and RFS in HCC. In vitro experiments validated that miR‐137 could bind to 3′‐untranslated region of the AFM and promote the invasion and metastasis of HCC cell lines. The expressions of miR‐137 and its liver microenvironment regulatory target gene AFM in combination significantly correlated with HCC progression in a higher number of patients than in previous studies, which suggested their potential as prognostic biomarkers for HCC.
Inhibition of MUC1‐C regulates metabolism by AKT pathway in esophageal squamous cell carcinoma
Authors: GongSun Xin , Zhao YongQiang , Jiang Bin , Xin ZhongWei , Shi Mo , Song Liang , Qin QiMing , Wang Qiang , Liu XiangYan 
Published: December 2018

AbstractEsophageal squamous cell carcinoma (ESCC) is one of the most common digestive tumors worldwide. The Mucin 1 (MUC1) heterodimeric protein has been confirmed that is overexpressed in ESCC and induced adverse outcomes. However, the detailed mechanism(s) remained challenging. So, we investigated the relationship between MUC1‐C and metabolism in ESCC cells. In the results, TP53‐induced glycolysis and apoptosis regulator (TIGAR) was overexpressed and correlative with MUC1‐C positively in ESCC tissue. Targeting MUC1‐C inhibits AKT–mTORC–S6K1 signaling and blocks TIGAR translation. We found that the inhibitory effect of GO‐203 on TIGAR was mediated by inhibition of AKT–mTOR–S6K1 pathway. The findings also demonstrated that the suppressive effect of GO‐203 on TIGAR is related to the decrease of glutathione level, the increase of reactive oxygen species and the loss of mitochondrial transmembrane membrane potential. In xenograft tissues, GO‐203 inhibited the growth of ESCC cells and lead to the low expression of transmembrane C‐terminal subunit (MUC1‐C) and TIGAR. This evidence supports the contention that MUC1‐C is significant for metabolism in ESCC and indicated that MUC1‐C is a potential target for the treatment of ESCC.
Dose‐ and time‐dependent alterations in lipid metabolism after pharmacological PGC‐1α activation in L6 myotubes
Authors: Supruniuk Elżbieta , Mikłosz Agnieszka , Chabowski Adrian , Łukaszuk Bartłomiej 
Published: December 2018

AbstractPyrroloquinoline quinone (PQQ) acts as a powerful modulator of PGC‐1α activation and therefore regulates multiple pathways involved in cellular energy homeostasis. In the present study, we assessed the effects of L6 myotubes incubation with 0.5, 1, and 3 μM PQQ solution for 2 and 24 hr with respect to the cells' lipid metabolism. We demonstrated that PQQ significantly elevates PGC‐1α content in a dose‐ and time‐dependent manner with the highest efficiency for 0.5 and 1 µM. The level of free fatty acids was diminished (24 hr: −66%), while an increase in triacylglycerol (TAG) amount was most pronounced after 0.5 μM (2 hr: +93%, 24 hr: +139%) treatment. Ceramide (CER) content was elevated after 2 hr incubation with 0.5 µM and after prolonged exposure to all PQQ concentrations. The cells treated with PQQ for 2 hr exhibited decreased sphinganine (SFA) and sphinganine‐1‐phosphate (SFA1P) level, while 24 hr incubation resulted in an elevated sphingosine (SFO) amount. In summary, PGC‐1α activation promotes TAG and CER synthesis.