bluuebunny/crossref_metadata_2025_split huggingface dataset:
The interplay between microbiota‐dependent metabolite trimethylamine N‐oxide, Transforming growth factor β/SMAD signaling and inflammasome activation in chronic kidney disease patients: A new mechanistic perspective
Authors: El‐Deeb Omnia Safwat , Atef Marwa Mohamed , Hafez Yasser Mostafa 
Published: April 2019

AbstractBackgroundChronic kidney disease (CKD) signifies a frequently life‐threatening condition influencing kidney structure and function. Despite its irrefutable importance, its exact pathogenesis is not completely clarified. However, CKD is known to be associated with accumulated uremic toxins/metabolites, interstitial fibrosis, and systemic inflammation. So we aimed to investigate the role of microbiota‐dependent metabolite trimethylamine N‐oxide (TMAO), transforming growth factor β (TGFβ)/SMAD signaling, and inflammasome activation in CKD pathogenesis through its different stages.Subjects and MethodsEighty patients with CKD of stages 2 to 4 in addition 15 healthy control subjects were enrolled. SMAD3 and nucleotide‐binding oligomerization domain‐, leucine‐rich repeat‐ and pyrin domain‐containing 3 (NLRP3) messenger RNA (mRNA) expressions from whole blood were assessed by quantitative real‐time polymerase chain reaction (RT‐PCR). Serum TGF‐β1 and interleukin‐1β (IL‐1β) levels were estimated by the enzyme‐linked immunosorbent assay. Plasma and urinary TMAO levels were measured. Oxidative stress markers were also assessed.ResultsSMAD3 and NLRP3 mRNA expressions were significantly upregulated in patients with CKD. Likewise, serum TGF‐β1 and IL‐1β levels were significantly elevated in patients with CKD, with increase in plasma and urinary TMAO levels and altered redox status throughout different CKD stages.ConclusionThe study documented that TMAO could be used as a reliable biomarker to evaluate CKD progression; being linked to TGF‐β/SMAD signaling, NLRP3 inflammasome activation as well as being a noninvasive applicable technique.
l‐Methionine activates Nrf2‐ARE pathway to induce endogenous antioxidant activity for depressing ROS‐derived oxidative stress in growing rats
Authors: Wang Zhengxuan , Liang Mingcai , Li Hui , Cai Liang , He Hongjuan , Wu Qiong , Yang Lin 
Published: April 2019

AbstractBACKGROUNDMethionine is an essential sulfur‐containing amino acid. To elucidate the influence of l‐methionine on activation of the nuclear factor erythroid 2‐related factor 2–antioxidant responsive element (Nrf2‐ARE) antioxidant pathway to stimulate the endogenous antioxidant activity for depressing reactive oxygen species (ROS)‐derived oxidative stress, male Wistar rats were orally administered l‐methionine daily for 14 days.RESULTSWith the intake of l‐methionine, Nrf2 was activated by l‐methionine through depressing Keap1 and Cul3, resulting in upregulation of ARE‐driven antioxidant expression (glutamate cysteine ligase catalytic subunit, glutamate cysteine ligase modulatory subunit, glutathione synthase (GS), catalase (CAT), superoxide dismutase (SOD), heme oxygenase 1, NAD(P)H:quinone oxidoreductase 1, glutathione reductase (GR), glutathione S‐transferase (GST), glutathione peroxidase (GPx)) with increasing l‐methionine availability. Upon activation of Nrf2, glutathione synthesis was increased through upregulated expression of methionine adenosyltransferase, S‐adenosylhomocysteine hydrolase, cystathionine β‐synthase, cystathionine γ‐lyse, glutamate cysteine ligase (GCL) and GS, while hepatic expressions of methionine sulfoxide reductases (MsrA, MsrB2, MsrB3) and hepatic enzyme activities (CAT, SOD, GCL, GR, GST, GPx) were uniformly stimulated with increasing consumption of l‐methionine. As a result, hepatic content of ROS and MDA were effectively reduced by l‐methionine intake.CONCLUSIONThe present study demonstrates that methionine availability plays a critical role in activation of the Nrf2‐ARE pathway to induce an endogenous antioxidant response for depressing ROS‐derived oxidative stress, which is primarily attributed to the stimulation of methionine sulfoxide reductase expression and glutathione synthesis. © 2019 Society of Chemical Industry
Root inoculation of green bell pepper (Capsicum annum) with Bacillus amyloliquefaciens BBC047: effect on biochemical composition and antioxidant capacity
Authors: Cisternas‐Jamet Jonathan , Salvatierra‐Martínez Ricardo , Vega‐Gálvez Antonio , Uribe Elsa , Goñi María G , Stoll Alexandra 
Published: April 2019

AbstractBACKGROUNDConsumption of bell peppers is recommended because of their bioactive compound content and their positive effects on health. Growth‐promoting rhizobacteria are popular because of their ability to promote plant growth by improving the fixation of nutrients or by inducing a systemic response. Green bell pepper (Capsicum annum) roots were inoculated with an autochthonous strain of Bacillus amyloliquefaciens, at different stages of development: T1, inoculation in the seedbed before transplant; T2, inoculation at and after transplant; T3, inoculation in the seedbed, at and after transplant. Bell pepper plants without inoculation were considered as control. Physicochemical composition and antioxidant activity of the fruits were measured to select the best treatment.RESULTST1 increased crude proteins, fat, Ca, Fe, vitamin C, total phenolic content, antioxidant capacity by DPPH and by ORAC. On the other hand, T1 decreased reducing sugars, K and Cu content. No significant differences for total carbohydrates, ash and photosynthetic pigments were found.CONCLUSIONInoculated green bell peppers have enhanced its functional value and could be considered as an important source of bioactive compounds with elevated antioxidant activity. © 2019 Society of Chemical Industry
Minimally invasive versus open distal pancreatectomy for pancreatic neuroendocrine tumors: An analysis from the U.S. neuroendocrine tumor study group
Authors: Zhang Xu‐Feng , Lopez‐Aguiar Alexandra G. , Poultsides George , Makris Eleftherios , Rocha Flavio , Kanji Zaheer , Weber Sharon , Fields Ryan , Krasnick Bradley A. , Idrees Kamran , Smith Paula M. , Cho Cliff , Schmidt Carl R. , Maithel Shishir K. , Pawlik Timothy M. ,   The United States Neuroendocrine Tumor Study Group
Published: April 2019

AbstractBackgroundTo determine short‐ and long‐term oncologic outcomes after minimally invasive distal pancreatectomy (MIDP) with open distal pancreatectomy (ODP) for the treatment of pancreatic neuroendocrine tumor (pNET).MethodsThe data of the patients who underwent curative MIDP or ODP for pNET between 2000 and 2016 were collected from a multi‐institutional database. Propensity score matching (PSM) was used to generate 1:1 matched patients with MIDP and ODP.ResultsA total of 576 patients undergoing curative DP for pNET were included. Two hundred and fourteen (37.2%) patients underwent MIDP, whereas 362 (62.8%) underwent ODP. MIDP was increasingly performed over time (2000‐2004: 9.3% vs 2013‐2016: 54.8%; P < 0.01). In the matched cohort (n = 141 in each group), patients who underwent MIDP had less blood loss (median, 100 vs 200 mL, P < 0.001), lower incidence of Clavien‐Dindo ≥ III complications (12.1% vs 24.8%, P = 0.026), and a shorter hospital stay versus ODP (median, 4 versus 7 days, P = 0.026). Patients who underwent MIDP had a lower incidence of recurrence (5‐year cumulative recurrence, 10.1% vs 31.1%, P < 0.001), yet equivalent overall survival (OS) rate (5‐year OS, 92.1% vs 90.9%, P = 0.550) compared with patients who underwent OPD.ConclusionPatients undergoing MIDP over ODP in the treatment of pNET had comparable oncologic surgical metrics, as well as similar long‐term OS.
Protective effects of the suppressed NF‐κB/TLR4 signaling pathway on oxidative stress of lung tissue in rat with acute lung injury
Authors: Zhang Ze‐Ming , Wang Yan‐Cun , Chen Lu , Li Zheng 
Published: April 2019

AbstractThe pathogenesis of acute lung injury (ALI) is characterized by lung inflammation and lung oxidative stress. The study was conducted in order to investigate the effect toll‐like receptor 4 (TLR4) and nuclear factor‐kappa B (NF‐κB) exhibited on oxidative stress in ALI. After the rats had been assigned into different groups, arterial blood, white blood cell (WBC), lung permeability index (LPI), wet/dry (W/D) ratio, TLR4 and NF‐κB expression and superoxide dismutase (SOD), myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH), and reactive oxygen species (ROS) were examined. Afterward, the correlation between the levels of TLR4 and NF‐κB was determined. Decreased levels of PaO2, SOD, MPO, and GSH accompanied by increased levels of PaCO2, WBC number, LPI and W/D ratio, MDA and ROS, as well as TLR4 and NF‐κB expressions in the ALI, ALI + NF‐κB inhibitor, and ALI + phosphate buffer saline groups were found. Inhibition of NF‐κB resulted in increased PaO2 and decreased PaCO2 levels, WBC number, and LPI and W/D ratio. Decreased expression of NF‐κB increased SOD, GSH, and MPO, but decreased MDA and ROS. We also found that NF‐κB inhibition resulted in the improvement of ALI in rats. TLR4 and NF‐κB expressions were negatively correlated with levels of SOD, MPO, and GSH, and positively correlated with MDA and ROS levels. In summary, our findings provided evidence that inhibition of the TLR4/NF‐κB signaling pathway decreases oxidative stress, thereby improving ALI. As a result, NF‐κB signaling pathway has shown potential as a therapeutic target in ALI therapy.
Microbial osteoporosis: The interplay between the gut microbiota and bones via host metabolism and immunity
Authors: Li Lishan , Rao Shitao , Cheng Yanzhen , Zhuo Xiaoyun , Deng Caihong , Xu Ningning , Zhang Hua , Yang Li 
Published: April 2019

AbstractThe complex relationship between intestinal microbiota and host is a novel field in recent years. A large number of studies are being conducted on the relationship between intestinal microbiota and bone metabolism. Bone metabolism consisted of bone absorption and formation exists in the whole process of human growth and development. The nutrient components, inflammatory factors, and hormone environment play important roles in bone metabolism. Recently, intestinal microbiota has been found to influence bone metabolism via influencing the host metabolism, immune function, and hormone secretion. Here, we searched relevant literature on Pubmed and reviewed the effect of intestinal microbiota on bone metabolism through the three aspects, which may provide new ideas and targets for the clinical treatment of osteoporosis.
A risk assessment of automated treatment planning and recommendations for clinical deployment
Authors: Kisling Kelly , Johnson Jennifer L. , Simonds Hannah , Zhang Lifei , Jhingran Anuja , Beadle Beth M. , Burger Hester , du Toit Monique , Joubert Nanette , Makufa Remigio , Shaw William , Trauernicht Christoph , Balter Peter , Howell Rebecca M. , Schmeler Kathleen , Court Laurence 
Published: April 2019

PurposeTo assess the risk of failure of a recently developed automated treatment planning tool, the radiation planning assistant (RPA), and to determine the reduction in these risks with implementation of a quality assurance (QA) program specifically designed for the RPA.MethodsWe used failure mode and effects analysis (FMEA) to assess the risk of the RPA. The steps involved in the workflow of planning a four‐field box treatment of cervical cancer with the RPA were identified. Then, the potential failure modes at each step and their causes were identified and scored according to their likelihood of occurrence, severity, and likelihood of going undetected. Additionally, the impact of the components of the QA program on the detectability of the failure modes was assessed. The QA program was designed to supplement a clinic's standard QA processes and consisted of three components: (a) automatic, independent verification of the results of automated planning; (b) automatic comparison of treatment parameters to expected values; and (c) guided manual checks of the treatment plan. A risk priority number (RPN) was calculated for each potential failure mode with and without use of the QA program.ResultsIn the RPA automated treatment planning workflow, we identified 68 potential failure modes with 113 causes. The average RPN was 91 without the QA program and 68 with the QA program (maximum RPNs were 504 and 315, respectively). The reduction in RPN was due to an improvement in the likelihood of detecting failures, resulting in lower detectability scores. The top‐ranked failure modes included incorrect identification of the marked isocenter, inappropriate beam aperture definition, incorrect entry of the prescription into the RPA plan directive, and lack of a comprehensive plan review by the physician.ConclusionsUsing FMEA, we assessed the risks in the clinical deployment of an automated treatment planning workflow and showed that a specialized QA program for the RPA, which included automatic QA techniques, improved the detectability of failures, reducing this risk. However, some residual risks persisted, which were similar to those found in manual treatment planning, and human error remained a major cause of potential failures. Through the risk analysis process, we identified three key aspects of safe deployment of automated planning: (a) user training on potential failure modes; (b) comprehensive manual plan review by physicians and physicists; and (c) automated QA of the treatment plan.
Using Specialized Angling to Assess a Trophy Florida Bass Fishery at Calling Panther Lake, Mississippi
Authors: Hall Edward P. , Bonvechio Timothy F. , Shaw Stephanie L. , Allen Micheal S. , Brown Jerry , Pugh Larry 
Published: April 2019

Abstract
Angling for trophy Largemouth Bass Micropterus salmoides and Florida Bass M. salmoides floridanus supports popular fisheries, particularly in the southern United States, where conditions are favorable for growing large fish. Traditional standardized sampling gears, such as electrofishing and creel surveys, often prove ineffective at obtaining sample sizes that are adequate for assessing the quality and temporal trends in trophy fisheries. In this study, we assessed the trophy Florida Bass population in the newly impounded Calling Panther Lake (163 ha), Mississippi, by using a nontraditional, specialized angling technique, and we compared the results with data collected by the more traditional techniques of creel surveys and electrofishing. The specialized angling technique utilized a select few highly skilled and devoted anglers that targeted only the largest bass in the population. The specialized angling technique collected nearly 8 and 24 times more trophy Florida Bass (≥3.6 kg or ≥56 cm TL) than creel surveys and electrofishing, respectively. As a result, this was the only method that reliably collected enough trophy-sized fish to estimate the population size in Calling Panther Lake. Mark–recapture data were used to estimate an abundance of 150 trophy Florida Bass (95% confidence interval [CI] = 118–206) in 2011 (density = 0.92 fish/ha; 95% CI = 0.73–1.26). The use of data collected by specialized anglers provided vital information for the trophy segment of this bass population, which is often difficult for fisheries managers to obtain with traditional techniques.
A Comparison between Conventional Boat Electrofishing and the Electrified Dozer Trawl for Capturing Silver Carp in Tributaries of the Missouri River, Missouri
Authors: Hammen Jeremy J. , Pherigo Emily , Doyle Wyatt , Finley Jeff , Drews Kevin , Goeckler Jason M. 
Published: April 2019

Abstract
Silver Carp Hypophthalmichthys molitrix are elusive fish that can be difficult to capture with conventional management tools. New tools must be developed to help increase the capture of Silver Carp, thereby improving the understanding and management of the species. An electrified dozer trawl was developed in an attempt to increase the capture of Silver Carp while reducing the amount of sampling effort needed to successfully assess a population and inform management actions. We compared Silver Carp catch rates, sample run time, and Silver Carp length frequency distributions from the electrified dozer trawl with those from conventional boat electrofishing. Silver Carp capture was greater and sample time was shorter for the electrified dozer trawl compared to conventional boat electrofishing. Length frequency distributions were similar between gears. The electrified dozer trawl can shorten sample run times and potentially improve Silver Carp capture, thus facilitating management. Use of the electrified dozer trawl should be expanded beyond Missouri River tributaries to further understand the gear's potential for sampling Silver Carp in a diverse array of environments.
Evaluation of Tag Retention, Healing, Growth, and Behavior in Age-0 Muskellunge Following Acoustic Transmitter Implantation
Authors: Walton-Rabideau Sarah E. , Newell Mark , Jeanson Amanda L. , Lédée Elodie J. I. , Farrell John M. , Cooke Steven J. 
Published: April 2019

Abstract
The development of small acoustic transmitters has enabled researchers to monitor earlier life stages and smaller fish species than was previously possible. The underlying assumptions of any telemetry study are minimal tag loss and negligible effects on the behavior, survival, and growth of tagged individuals. To that end, tag retention, healing, survival, specific growth rates, and behavior were evaluated for 96 age-0 Muskellunge Esox masquinongy (TL [mean ± SD] = 205 ± 10 mm) from three treatment groups. Tagged fish were compared to untagged controls and sham fish (fish that had undergone anesthesia and laparotomy but not transmitter implantation). Thirty-two fish (tagged group) were implanted with one of the smallest commercially available acoustic transmitters (Juvenile Salmon Acoustic Telemetry tag; 12.0 × 5.3 × 3.7 mm, 0.217 g in air, &gt;120-d tag life) and monitored in a 4-month, overwinter tank experiment. Tricaine methanesulfonate was used for anesthesia, incisions were closed with a synthetic absorbable monofilament, and all surgeries were conducted by a single trained researcher. All tags were retained throughout the experiment; surgical wounds healed within 30 d, 32% of sutures were retained at 120 d postsurgery, and survival did not differ between treatments. No biologically significant effects of tagging on mean relative growth rates (percent change in weight/d) were observed among the three groups (tagged, untagged, and sham fish) at 4 months postprocessing. The reaction of tagged fish to a moving object within 15 minutes after tagging was slower than the reaction at 7 d postsurgery, reiterating the importance of testing appropriate sedation methods prior to releasing fish in field studies. Results validate the utility of surgical implantation of small acoustic transmitters in juvenile Muskellunge for future studies, although immobilization methods for early life stages require further study.
Robust optimization of dynamic route planning in same‐day delivery networks with one‐time observation of new demand
Authors: Yao Bing , McLean Caitlin , Yang Hui 
Published: April 2019

AbstractLocal delivery networks expect drivers to make deliveries to and/or pickups from customers using the shortest routes in order to minimize costs, delivery time, and environmental impact. However, in real‐world applications, it is often the case that not all customers are known when planning the initial delivery route. Instead, additional customers become known while the driver is making deliveries or pickups. Before serving the new demand requests, the vehicle will return to the depot for restocking. In other words, there exists a precedence relation in the delivery route to visit the depot before delivering new orders. The uncertainty in new customer locations can lead to expensive rerouting of the tour, as drivers revisit previous neighborhoods to serve the new customers. We address this issue by constructing the delivery route with the knowledge that additional customers will appear, using historical demand patterns to guide our predictions for the uncertainty. We model this network delivery problem as a precedence‐constrained asymmetric traveling salesman problem using mixed‐integer optimization. Experimental results show that the proposed robust optimization approach provides an effective delivery route under the uncertainty of customer demands.
Newly Defined ATP-Binding Cassette Subfamily B Member 5 Positive Dermal Mesenchymal Stem Cells Promote Healing of Chronic Iron-Overload Wounds via Secretion of Interleukin-1 Receptor Antagonist
Authors: Vander Beken Seppe , Vries Juliane C. , Meier-Schiesser Barbara , Meyer Patrick , Jiang Dongsheng , Sindrilaru Anca , Ferreira Filipa F. , Hainzl Adelheid , Schatz Susanne , Muschhammer Jana , Scheurmann Natalie J. , Kampilafkos Panagiotis , Seitz Andreas M. , Dürselen Lutz , Ignatius Anita , Kluth Mark A. , Ganss Christoph , Wlaschek Meinhard , Singh Karmveer , Maity Pallab , Frank Natasha Y. , Frank Markus H. , Scharffetter-Kochanek Karin 
Published: April 2019

Abstract
In this study, we report the beneficial effects of a newly identified dermal cell subpopulation expressing the ATP-binding cassette subfamily B member 5 (ABCB5) for the therapy of nonhealing wounds. Local administration of dermal ABCB5+-derived mesenchymal stem cells (MSCs) attenuated macrophage-dominated inflammation and thereby accelerated healing of full-thickness excisional wounds in the iron-overload mouse model mimicking the nonhealing state of human venous leg ulcers. The observed beneficial effects were due to interleukin-1 receptor antagonist (IL-1RA) secreted by ABCB5+-derived MSCs, which dampened inflammation and shifted the prevalence of unrestrained proinflammatory M1 macrophages toward repair promoting anti-inflammatory M2 macrophages at the wound site. The beneficial anti-inflammatory effect of IL-1RA released from ABCB5+-derived MSCs on human wound macrophages was conserved in humanized NOD-scid IL2rγnull mice. In conclusion, human dermal ABCB5+ cells represent a novel, easily accessible, and marker-enriched source of MSCs, which holds substantial promise to successfully treat chronic nonhealing wounds in humans. Stem Cells 2019;37:1057–1074
Reframing the Achievement Gap: Lessons From Puzzling Students
Authors: Ballenger Cynthia 
Published: April 2019

AbstractThe achievement gap has been the focus of a good deal of research, attention, and hopeful new practices, yet it has hardly decreased. Using insights from sociolinguistic and sociocultural studies of classrooms in concert with practices developed by teacher researchers in the tradition of the Brookline Teacher Researcher Seminar, the author proposes a radical reframing of the position, and of the intellectual strength, of students not successfully engaged in school by exploring reading group interactions, focusing on two of her students and her own response to what they said. Considering details from the talk of these students, the author suggests ways in which teachers often miss or misjudge the full understandings and the intellectually rich intentions of many ostensibly less successful students.
Values and Intercultural Experiences Through Picture Books
Authors: Tomé‐Fernández María , Senís‐Fernández Juan , Ruiz‐Martín Diego 
Published: April 2019

AbstractThe authors searched for picture books that are useful for intercultural education and for discovering students’ perceptions about the values and intercultural experiences conveyed through the books. To do so, the authors selected two books by following established criteria and analyzed students’ responses through semistructured interviews. The data were obtained after reading the books aloud to 30 fifth‐grade students in a school located in the south of Spain and reflecting on the books’ contents. As a main conclusion, the authors found that students’ answers revealed the presence of some positive values, such as helpfulness, friendship, and empathy, through questions about the experiences of characters in the books. Thus, prejudices were dismantled and positive intercultural experiences in school were fostered.