bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput sequencing reveals circular RNA hsa_circ_0000592 as a novel player in the carcinogenesis of gastric carcinoma
Authors: Liang Min , Liu Zhaoyu , Lin Hai , Shi Boyun , Li Ming , Chen Ting , Qin Lingyu , Niu Qiuling , Yu Guifang , Jiang Hui , Zhou Xinke 
Published: June 2019

Abstract
Background/Aim: Gastric cancer is one of the most common malignant tumors, and its complex pathogenesis has not been fully elucidated. Circular RNAs (circRNAs) are involved in various biological processes and human diseases. However, their exact functional roles and mechanisms of action remain largely unclear. We previously discovered the differential expression of non-coding RNAs (ncRNAs) during the malignant transformation of human gastric epithelial cells. In this study, we investigated the functional roles of a significantly up-regulated circRNA (hsa_circ_0000592) in gastric cancer. Methods:N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-induced malignant-transformed gastric epithelial cells (GES-1-T) and normal gastric epithelial cells (GES-1-N) were analyzed by high-throughput circRNA sequencing. The top 15 up-regulated circRNAs in high-throughput sequencing results were further confirmed by qRT-PCR in different gastric epithelial cell lines. The function of the most significant circRNA (hsa_circ_0000592) was investigated by using RNA interference (RNAi) assays, fluorescence in situ hybridization analysis (FISH), and bioinformatics prediction methods. Results: A total of 1509 genes were up-regulated and 3142 genes were down-regulated in GES-1-T cells when compared with GES-1-N cells. When compared with GES-1-N cells, hsa_circ_0000592 was obviously up-regulated in GES-1-T cells, as well as in other gastric cancer cell lines. The silencing of hsa_circ_0000592 mRNA led to a decrease in cell proliferation, cell cycle arrest at the G0/G1 phase, an increased rate of apoptosis, and a reduction in cell migration. Furthermore, FISH showed that hsa_circ_0000592 was mainly located in the cytoplasm, and a bioinformatics analysis suggested that hsa_circ_0000592 might function by sponging multiple miRNAs, and most notably four conserved miRNAs, including miR-139-3p, miR-200, miR-367-3p, and miR-33a-3p. Conclusion: This study is the first to identify hsa_circ_0000592 as a novel circRNA with a critical role in MNNG-induced gastric cancer. Due to the essential role of hsa_circ_0000592 in gastric carcinoma cells, it may be considered as a potential biomarker for use in diagnosing gastric carcinoma. Our findings provide a new insight into the function of circRNAs in environmental carcinogen-induced gastric cancer.
LncRNA ANRIL affects the sensitivity of ovarian cancer to cisplatin via regulation of let-7a/HMGA2 axis
Authors: Miao Jin-Tian , Gao Jian-Hua , Chen Yong-Qian , Chen Hong , Meng Hao-Yi , Lou Ge 
Published: June 2019

Abstract
This paper tried to explore ANRIL expression in ovarian cancer and how it affects cisplatin-sensitivity of ovarian cancer cells via regulation of let-7a/high-mobility group protein A2 (HMGA2) axis. qRT-PCR was used to detect ANRIL and let-7a levels in ovarian cancer tissues and cell lines (SKOV3 and SKOV3/DDP). Then cells were randomly assigned into Blank, negative control siRNA, ANRIL siRNA, let-7a inhibitor, and ANRIL siRNA+let-7a-inhibitor groups. CCK-8 assay was applied for assessing cell viability of cells treated with different concentrations of cisplatin. Flow cytometry was employed to test cell apoptosis rate. qRT-PCR and Western blot were performed for related molecules detection. Nude mice transplanted with SKOV3/DDP cells were used to confirm the effects of ANRIL siRNA on the cisplatin-sensitivity. Ovarian cancer tissues and cisplatin-resistant cells had increased ANRIL expression and decreased let-7a expression, and those patients with higher clinical stage and pathological grade showed higher ANRIL and lower let-7a. Dual-luciferase reporter-gene assay confirmed the targeting relationship between ANRIL and let-7a, and between let-7a and HMGA2. The cell viability and cisplatin IC50 were decreased in ANRIL siRNA group exposed to different concentrations of cisplatin, with enhanced apoptosis, as well as elevated let-7a and declined HMGA2, which would be reversed by let-7a inhibitor. Meanwhile, ANRIL down-regulation enhanced the inhibitory effect of cisplatin on tumor growth of nude mice and reduced tumor weight. Silencing ANRIL expression reduced HMGA2 expression to promote the apoptosis and improve cisplatin-sensitivity of ovarian cancer cells via up-regulating let-7a expression.
microRNA-613 exerts anti-angiogenic effect on nasopharyngeal carcinoma cells through inactivating the AKT signaling pathway by down-regulating FN1
Authors: Gao Ru , Feng Qiaolei , Tan Guolin 
Published: June 2019

Abstract
Background: Nasopharyngeal carcinoma (NPC) is a disease highly sensitive to radiotherapy with the unclear etiology. However, the specific effects of microRNA-613 (miR-613) on NPC still remain elusive. Therefore, the present study probes into the underlying mechanism of miR-613 in NPC via AKT signaling pathway by regulating Fibronectin 1 (FN1).
Methods: First, microarray analysis was used to screen differentially expressed genes (DEGs) and regulatory miRs associated with NPC. Next, miR-613 and FN1 expression in NPC cells was determined, followed by verification of target relationship between miR-613 and FN1. With NPC cells exposed to miR-613 mimic, si-FN1 and LY294002 (inhibitor of AKT signaling pathway), the regulatory effects of miR-613 on proliferation, apoptosis, invasion, migration and angiogenesis of NPC cells were detected with ratio of B-cell lymphoma 2/Bcl-2-associated X protein (Bcl-2/Bax), Cleaved-caspase3, matrix metallopeptidase 2 (MMP-2), MMP-9, vascular endothelial growth factor (VEGF), and cell adhesion molecule-1 (CD31) expression measured. Then, tumorigenesis and MVD were determined after Xenograft in nude mice.
Results: FN1 modulated by miR-613 was critical for NPC via the AKT signaling pathway. NPC cells exhibited down-regulated miR-613 and up-regulated FN1. Besides, miR-613 was verified to target FN1. Moreover, overexpressed miR-613, silenced FN1 or LY294002 treatment suppressed proliferation, invasion, migration, and angiogenesis in NPC cells, which was indicated by reduced expression of AKT, mTOR, MMP-2, MMP-9, VEGF, and CD31 as well as decreased ratio of Bcl-2/Bax and increased expression of Cleaved-caspase3. Furthermore, cell apoptosis was promoted and tumorigenesis and MVD in nude mice were inhibited with overexpression of miR-613, silenced FN1 or LY294002 treatment.
Conclusion: Taken together, miR-613 inhibits angiogenesis in NPC cells through inactivating FN1-dependent AKT signaling pathway.
Trunk Muscle Aerobic Metabolism Responses in Endurance Athletes, Combat Athletes and Untrained Men
Authors: Anthierens Agathe , Olivier Nicolas , Thevenon André , Mucci Patrick 
Published: June 2019

AbstractThis study investigated aerobic metabolism responses in trunk muscles during a prolonged trunk extension exercise in athletes and untrained young men. The aim was to analyze the adaptations induced by 2 types of sports: one involving intensive use of trunk muscles (i. e., judo), and one known to induce high aerobic capacity in the whole body (i. e., cycling). Eleven judokas, 10 cyclists and 9 healthy untrained young men performed trunk extension exercises on an isokinetic dynamometer. During the first session, muscle strength was assessed during maximal trunk extension. During a second session, a 5-min exercise was performed to investigate aerobic responses with regard to trunk muscles. The near infrared spectroscopy technique and a gas exchange analyzer were used continuously to evaluate mechanical efficiency, V̇O2 on-set kinetics, trunk muscle deoxygenation and blood volume. Judokas showed greater trunk strength and mechanical efficiency (p<0.05). Cyclists presented faster V̇O2 on-set kinetics (p<0.05) and greater muscle deoxygenation and blood volume compared to untrained men (p<0.001). These results suggest that practicing judo improves trunk extension efficiency whereas cycling accelerates aerobic pathways and enhances microvascular responses to trunk extension exercise. Sport practice improves aerobic metabolism responses in trunk extensor muscles differently, according to the training specificities.
Heart–Lung Interaction
Authors: Kochhar Gaurav , Mehta Yatin , Trehan Naresh 
Published: June 2019

AbstractMechanical interplay between respiratory and cardiac systems was first recognized about 300 years ago when an English physiologist Stephen Hales observed that the level of the blood column in a glass tube inserted into the carotid artery of a horse varied cyclically with respiration.Heart and lung share the same intrathoracic space, and mechanically this configuration is akin to pump within a pump. As a result, intrathoracic pressure (ITP) and volume changes during respiratory cycle affect the performance of heart. Besides direct mechanical effects, lung and heart interplay also involves certain neurally and humorally mediated phenomena. Taken together, these dynamics constitute heart–lung interaction. Knowledge of heart–lung interaction is especially useful while dealing with critically sick patients on ventilator, because not only are such patients more vulnerable to hemodynamic instability induced by ventilator, but it also forms the basis of functional hemodynamic monitoring.
Intracardiac Repair of the Tetralogy of Fallot Using Trans-right Atrial and Transpulmonary Approach: A Video Presentation
Authors: Sankhyan Lakshmi Kumari , Chowdhury Ujjwal Kumar , George Niwin , Chauhan Abhinavsingh , Kapoor Poonam Malhotra 
Published: June 2019

AbstractTetralogy of Fallot (TOF) is one of the common cyanotic heart diseases. Now total repair is increasingly being used to save children at younger age and having lower body weight. The aim of this study was to evaluate the results of the two different surgical techniques used for total repair of TOF: transatrial-transpulmonary approach and transventricular approach with special emphasis on pre- and intraoperative risk factors that affect the prognosis of patients, and with analysis of postoperative short-term results.
The Angelina Dissectors: A Novel Design of Dissectors for Endoscopic Endonasal Approaches
Authors: Todeschini Alexandre B. , Otto Bradley A. , Carrau Ricardo L. , Prevedello Daniel M. 
Published: June 2019

Background The description and refinement of the transsphenoidal approach would not be possible without new tools and technologies developed by surgeons to facilitate this approach, which is nowadays the standard procedure for more than 90% of sellar lesions. The latest major change in transsphenoidal surgery was the introduction of the rigid endoscope and the subsequent description of the endoscopic endonasal approach. Traditional bayoneted instruments, when used for this technique, were inadequate. New instruments designed, specifically for this technique, are necessary to facilitate the surgeon's work and improve patient outcome.
Objective This study describes a novel design of dissectors created specifically for endoscopic endonasal approaches.
Methods To develop and design the Angelina dissectors, we used our extensive surgical experience to identify the shortcomings of the available dissectors used for transsphenoidal surgery and created the Angelina dissectors.
Results The Angelina dissector was designed with a unique shaft shape which facilitates endoscopic endonasal surgery.
Conclusion Even though an endoscopic endonasal approach is possible using other instruments, the design of these dissectors aids the surgeon's work. It is our impression, based on personal experience that it allows more freedom of movement and dexterity during the procedure, which could translate as an improved patient outcome.
Second type of criticality in the brain uncovers rich multiple-neuron dynamics
Authors: Dahmen David , Grün Sonja , Diesmann Markus , Helias Moritz 
Published: June 2019

SignificanceParallel recordings of motor cortex show weak pairwise correlations on average but a wide dispersion across cells. This observation runs counter to the prevailing notion that optimal information processing requires networks to operate at a critical point, entailing strong correlations. We here reconcile this apparent contradiction by showing that the observed structure of correlations is consistent with network models that operate close to a critical point of a different nature than previously considered: dynamics that is dominated by inhibition yet nearly unstable due to heterogeneous connectivity. Our findings provide a different perspective on criticality in neural systems: network topology and heterogeneity endow the brain with two complementary substrates for critical dynamics of largely different complexities.
An endosomal LAPF is required for macrophage endocytosis and elimination of bacteria
Authors: Li Tianliang , Qin Kewei , Li Nan , Han Chaofeng , Cao Xuetao 
Published: June 2019

Significance
Host innate immune cells, such as macrophages, can endocytose the invading pathogens and induce inflammatory innate responses to fight infection. In this study, we show that a Cav1-interacting protein, LAPF, promotes the endocytosis of bacteria and production of proinflammatory cytokines by inducing Src-LAPF-Caveolin complex formation in response to innate stimuli. Our results reveal a host defense strategy against bacterial infection by increasing macrophage endocytosis and induction of innate response. We propose that pharmacological activation of LAPF and Src could potentially be applied to the control of bacterial infections.
Explicit and implicit discursive strategies and moral order in a trial process
Authors: Moulinou Iphigenia 
Published: June 2019

AbstractThe present paper examines data which has been drawn from the official proceedings of a murder trial in a Greek court, concerning the killing of an adolescent by a police officer (see alsoGeorgalidou 2012,2016), and addresses the issues of aggressive discursive strategies and the moral order in the trial process. It analyses the explicit and implicit strategies involved in morally discrediting the opponent, a rather frequent defence strategy (Atkinson and Drew 1979;Coulthard and Johnson 2007;Levinson 1979). The paper examines agency deflection towards the victim (Georgalidou 2016), attribution of a socio-spatial identity to the victim and witnesses in an essentialist and reductionist way, and other linguistic and discursive means, the majority of which mobilize moral panic and have implications for the moral order in court. I argue that these aggressive discursive means primarily contribute to the construction of a normative moral order by both adversarial parties.
Creole typology is analytic typology
Authors: Szeto Pui Yiu , Lai Jackie Yan-ki , Ansaldo Umberto 
Published: June 2019

AbstractThis paper reviews a number of specific features typical of analytic languages, in an attempt to investigate whether Creole languages can indeed be grouped, at least structurally, with other languages of the analytic (or isolating) type. Based onSybesma et al. (forthcoming), a study of the nature of analyticity, we select eight features which constitute rather obvious structural parallels between two unrelated groups, namely Sinitic and Kwa. In terms of Creole languages, these eight features can be also clearly located within the APiCS (Michaelis et al. 2013). Contrary to works likeBakker et al. (2011)which argue for the existence of a “Creole Prototype”, our results show that Creole languages do not cluster with each other against other non-Creole languages. Instead, various Creoles clearly owe their grammatical profile to the languages that dominate the typological environment in which they are formed.
The long and short of it
Authors: Nash Joshua 
Published: June 2019

AbstractPlacenames (toponyms) give insight into relationships involving people, place, and language. An exemplary placename derived from long-term engagement within the sensitive linguistic ecology of Norfolk Island in the South Pacific is used to detail how a fusing of linguistic analysis, words, and cultural memory is beneficial for what constitutes an ecolinguistic fieldwork methodology. Differences between the ethnographic method and an ecolinguistic fieldwork methodology are presented. This enduring and keyed-in commitment with Norfolk Island’s social and natural surroundings offers significant perceptiveness into and suggestions about how prolonged ecolinguistic work can be beneficial to language documentation projects, particular those incorporating lexical (word) and semantic (memory) description.
From speaker innovation to lexical change
Authors: Nevalainen Terttu 
Published: June 2019

AbstractApplying a sociolinguistic approach to the study of neologisms, this paper discusses the actuation and diffusion of new words in Early Modern English (EModE; 1500–1700) and draws some parallels with word coining in the comparable but more recent period of Early Modern Finnish (EModF; 1810–1880). The success of this exercise ultimately depends on the data and tools available for ascertaining the status of neologisms in a broader synchronic and diachronic context. The use of historical dictionaries and digital databases shows that many words that were earlier considered particular innovations in EModE are now up for re-evaluation. Determining their actual moments of coinage and entry into the language may be beyond historical lexicology and lexicography, but the new tools make it possible to better monitor their provenance and process of diffusion over time.
Two types of morphologically expressed non-verbal predication
Authors: Bertinetto Pier Marco , Ciucci Luca , Farina Margherita 
Published: June 2019

AbstractThe morphological expression of non-verbal predication is a geographically widespread, although not very frequent, typological feature. This paper highlights the existence of two radically contrasting types of non-verbal predicative inflection. Construction A has already been described in the literature. It consists of attaching person-sensitive inflection markers to non-verbal predicates, possibly extending this treatment to adverbs and adverbial phrases (locational and temporal), pronouns and quantifiers. This type is well attested in Uralic, Turkic, and Paleosiberian, as well as in some Amazonian language families (most notably Chicham), but it has also been pointed out for some sparse languages of Oceania and Africa. Such non-verbal person inflections diachronically stem from incorporation of conjugated copula elements. Construction B, by contrast, is much rarer and is described here for the first time. It also consists of a dedicated morphological form of the non-verbal predicate (limited, however, to nouns and adjectives), but such form stands out as morphologically lighter than any other form to be found in nouns or adjectives in argument or attribute position. While the latter forms carry some kind of case marker, the noun/adjective predicate merely consists (or historically did) of the word’s root. This type of construction can be found in the small Zamucoan family and still survives in some Tupí-Guaraní languages. Diachronic inspection of Semitic indicates, however, that this predicative strategy was possibly adopted in some ancient varieties, although at later stages it intertwined with the expression of referential specificity. The paper compares the two construction types, highlighting similarities and differences.
Information system design using labor productivity measurement for construction
Authors: ‘Azzam Abdullah , Miranda Suci , Indrawati Sri 
Published: June 2019

Abstract
Labor utilization in construction project is the most critical because it costs 30-50% of the total cost. In many projects, the labors e.g craftman are hired outside the company. Contractors and owners need sufficient information related to those labors whom they will work with. It is necessary for clients understanding each labor productivity performance in order to achieve the project quality. This study aims to provide the infomation needed by designing an information system that can be easily accessed. This is also to support clients for decision making in terms of teamwork project involved. The level of productivity is measured by Labor Utilization Rate (LUR) using three variables: effective working time, contributory working time, and ineffective working time. Software Development Life Cycle (SDLC) is applied in designing the information system. The finding showed that information system is properly implemented to assist clients in determining workers using productivity calculation. The system can inform the recommended workers by calculating and sorting their level of productivity that support client’s decision making towards construction workers.
Development of Information Systems as a Means to Improve Sharia Cooperative Services
Authors: Safitri Riri , Haryadi Dody , Sulisthyani Endah , Noorliko Vareza 
Published: June 2019

Abstract
Increasing and empowering the economy of the community is an important thing that is a shared responsibility. This is the basis for the LAZ Al Azhar Empowerment “Sejuta Berdaya” program that makes Jampang Village as one of the target groups in the form of Kelompok Swadaya Masyarakat (KSM) which are named KSM Pelita Jampang Gemilang. The community in Jampang village has a variety of jobs with a low economic level. The foundation provides funding to the community through a sharia microfinance institution that collects and distributes public funds in accordance with Islamic law. The Jampang Sharia Cooperative is still operating manually. Registration process, member information, amount of funds and reports are still done manually and stored in a notebook. This causes the operational process not to run properly, because the data is not organized and stored neatly. Based on these conditions, a financial information system is needed, which is a web-based sharia cooperative, so that the operational processes and reports to members and administrators can run better and faster, so that they can help effectively and on target. Information systems are created using SDLC through the stages of user requirements, analysis and implementation.
Best Concept Selection for Dry-Soybean Cracking Machine Process Optimization using TOPSIS method
Authors: Anugraha R A , Darmawan N M , Iqbal M 
Published: June 2019

Abstract
Tempe is a traditional Indonesian food with processed soybean as the main ingredients. Indonesia is the largest tempe producer in the world with hundreds of thousands of traditional tempe maker. Currently the traditional production process is wasteful that requires the amount of water as much as 1321 liters/production of 37,5 kg tempe. Innovative dry production process is still less desirable because the dry soybean cracker machine is not optimal with a defect of 14,4% per production. In addition, the machine is unable to separate the husk and soybeans from the outlet. There are 12 alternative production concepts to produce highest quality tempe. TOPSIS method was used for selecting best production concept which reduces defect to 6,5% while manual separation process is eliminated. Water consumption is reduced to 800 liters/production of 35,125 kg tempe.