bluuebunny/crossref_metadata_2025_split huggingface dataset:
QbD-driven Formulation Development and Evaluation of Genistein
Nanoparticles for Prostate Cancer
Authors: Patel Nirav , Patel Priya 
Published: October 2024

Background:
Genistein (GEN) shows significant anticancer potential, particularly
against prostate cancer. However, its clinical application is limited by poor water solubility, rapid
metabolism and excretion, low bioavailability, and lack of targeted delivery to cancer cells, hindering
its effectiveness as a chemopreventive or therapeutic agent.

Objective:
In this study, poly-ε-caprolactone (PCL) nanoparticles incorporating polyvinyl alcohol
(PVA) as a stabilizer were engineered to encapsulate genistein (GEN) effectively. Utilizing a Quality
by Design (QbD) methodology, the development and optimization of these nanoparticles were
systematically approached.

Methods:
GEN-loaded PCL nanoparticles (NPs) were prepared using the Solvent Evaporation
Technique, ideal for encapsulating hydrophobic drugs. A Plackett–Burman design (PBD) identified
key factors, followed by a Box–Behnken design (BBD) to optimize nanoparticle quality. The NPs
were evaluated for particle size, zeta potential (ZP), polydispersity index (PDI), morphology, encapsulation
efficiency (EE), in vitro drug release, and cytotoxicity.

Results:
The optimized formulation containing PCL, PVA, and Volume of organic solvent as 43.7
mg, 6.2 mg, and 10.0 ml, respectively was chosen because it showed EE (%) of 94.0%, average particle
size of 150 nm, PDI of 0.10, ZP of -28.0 and exhibited sustained release of GEN for around
four days. The antiproliferative activities of GEN PCL NPs were confirmed by the MTT test in
vitro on malignant prostate carcinoma cell lines (PC3). Flow cytometric analysis showed that the
inhibition of cell proliferation of more potent GEN PCL NPs is comparable with the effects of free
GEN.

Conclusion:
The findings indicate that genistein-loaded PCL nanoparticles have the potential to
augment the anticancer efficacy of genistein, both in vitro and in vivo. This suggests their promise
as a viable candidate for prostate cancer treatment.
Self-efficacy and quality of life among patients undergoing continuous ambulatory peritoneal dialysis
Authors: Enikmawati Anik , Hastuti Weni , Purwaningsih Heni , Enawati Sri , Yuningsih Dwi , Ratnasari Nita Yunianti 
Published: October 2024

<p><strong><em>Background: </em></strong><em>Continuous Ambulatory Peritoneal Dialysis (CAPD) is a form of chronic peritoneal dialysis for patients with chronic kidney disease. It is carried out 3-5 times per day, 7 days per week with each time the dialysis fluid in the peritoneal cavity (dwelltime) for more than 4 hours. Through so many procedures to go through in each dialysis, it is not uncommon to affect the patient's quality of life.</em><em></em></p><p><strong><em>Objectives:</em></strong><em> </em><em>To find out the correlation between self-efficacy and the quality of life among patients undergoing continuous ambulatory peritoneal dialysis.</em></p><p><strong><em>Methods:</em></strong><em> </em><em>This study is quantitative research with a cross-sectional research design. The population employed all chronic kidney failure patients undergoing CAPD at Kasih Ibu Surakarta Hospital in 2023, with a total of 42 patients. The sample involved 42 samples selected using total sampling technique. Data collection used a self-efficacy questionnaire and quality of life questionnaire which its test consisted of univariate and bivariate using Chi-square test</em><em></em></p><p><strong><em>Results: </em></strong><em>The number of respondents having self-efficacy in the good category as many as 25 respondents (59,5%); the number of respondents with a quality of life in the good category is 29 respondents (69,0%); </em><em>the Chi Square test results reveal that the amount of p-value obtained is 0,011 (p-value less than 0,05) means there is a correlation between self-efficacy and the quality of life among patients undergoing Continous Ambulatory Peritoneal Dialysis (CAPD) at Kasih Ibu Surakarta Hospital.</em></p><p><strong><em>Conclusions:</em></strong><em> </em><em>There is a correlation between self-efficacy and the quality of life among patients undergoing Continous Ambulatory Peritoneal Dialysis (CAPD) at Kasih Ibu Surakarta Hospital with a p-value of 0,018 (&lt;0,05)</em><em>. </em></p>
Leveraging Artificial Intelligence and Data Science for Integration of Social Determinants of Health in Emergency Medicine: Scoping Review
Authors: Abbott Ethan E , Apakama Donald , Richardson Lynne D , Chan Lili , Nadkarni Girish N 
Published: October 2024

Abstract

Background
Social determinants of health (SDOH) are critical drivers of health disparities and patient outcomes. However, accessing and collecting patient-level SDOH data can be operationally challenging in the emergency department (ED) clinical setting, requiring innovative approaches.

Objective
This scoping review examines the potential of AI and data science for modeling, extraction, and incorporation of SDOH data specifically within EDs, further identifying areas for advancement and investigation.

Methods
We conducted a standardized search for studies published between 2015 and 2022, across Medline (Ovid), Embase (Ovid), CINAHL, Web of Science, and ERIC databases. We focused on identifying studies using AI or data science related to SDOH within emergency care contexts or conditions. Two specialized reviewers in emergency medicine (EM) and clinical informatics independently assessed each article, resolving discrepancies through iterative reviews and discussion. We then extracted data covering study details, methodologies, patient demographics, care settings, and principal outcomes.

Results
Of the 1047 studies screened, 26 met the inclusion criteria. Notably, 9 out of 26 (35%) studies were solely concentrated on ED patients. Conditions studied spanned broad EM complaints and included sepsis, acute myocardial infarction, and asthma. The majority of studies (n=16) explored multiple SDOH domains, with homelessness/housing insecurity and neighborhood/built environment predominating. Machine learning (ML) techniques were used in 23 of 26 studies, with natural language processing (NLP) being the most commonly used approach (n=11). Rule-based NLP (n=5), deep learning (n=2), and pattern matching (n=4) were the most commonly used NLP techniques. NLP models in the reviewed studies displayed significant predictive performance with outcomes, with F1-scores ranging between 0.40 and 0.75 and specificities nearing 95.9%.

Conclusions
Although in its infancy, the convergence of AI and data science techniques, especially ML and NLP, with SDOH in EM offers transformative possibilities for better usage and integration of social data into clinical care and research. With a significant focus on the ED and notable NLP model performance, there is an imperative to standardize SDOH data collection, refine algorithms for diverse patient groups, and champion interdisciplinary synergies. These efforts aim to harness SDOH data optimally, enhancing patient care and mitigating health disparities. Our research underscores the vital need for continued investigation in this domain.
Evaluating the Impacts of Community-Campus Engagement on Population Health in Ottawa and Thunder Bay, Canada: Protocol for a Mixed Methods Contribution Analysis
Authors: Buetti David , Larche Cynthia , Fitzgerald Michael , Bourgeois Isabelle , Cameron Erin , Carr Kady , Aubry Tim , Persaud Sydney , Kendall Claire E 
Published: October 2024

Background
Municipalities play a crucial role in population health due to their community connections and influence on health determinants. Community-campus engagement (CCE), that is, collaboration between academic institutions and communities, is a promising approach to addressing community health priorities. However, evidence of CCE’s impact on population health remains limited. Measuring the impacts of CCE is inherently complex due to factors such as diverse stakeholders, context-specific variables, and dynamic interactions within a community.

Objective
This study aims to develop robust evidence on the impacts of CCE on population health outcomes in Ottawa and Thunder Bay, Ontario, Canada, focusing on 5 shared health priorities: housing, discrimination, poverty, violence, and mental health.

Methods
We will use a proven CCE model called CityStudio, which has been implemented in both cities. We will use Mayne's mixed methods contribution analysis in three stages: (1) formulating a theory of change that outlines the expected contributions of CCE to population health outcomes; (2) gathering qualitative and quantitative data in line with the established Theory of Change; the data will be collected from various sources, including case studies of existing CityStudio projects, a web-based CCE stakeholder survey, a literature review, and population and community health data; and (3) reviewing the gathered evidence to determine the extent of CCE impacts on population health.

Results
Ethical approval for this project was granted in May 2023. We have since initiated stage 1 by reviewing the literature to inform the development of the theory of change. We expect to complete this study by May 2026.

Conclusions
This study will address two critical gaps about how improving health outcomes depends on CCE: (1) how academic institutions can best engage with their communities to improve population health outcomes, and (2) how municipalities can engage with academic institutions to address their community health priorities. Conducting our work in differing contexts will allow us to consider a broader range of other influences on outcomes, thus making our work applicable to various settings and outcomes.

International Registered Report Identifier (IRRID)
PRR1-10.2196/58546
Accuracy of the Huawei GT2 Smartwatch for Measuring Physical Activity and Sleep Among Adults During Daily Life: Instrument Validation Study
Authors: Mei Longfei , He Ziwei , Hu Liang 
Published: October 2024

Abstract

Background
Smartwatches are increasingly popular for physical activity and health promotion. However, ongoing validation studies on commercial smartwatches are still needed to ensure their accuracy in assessing daily activity levels, which is important for both promoting activity-related health behaviors and serving research purposes.

Objective
This study aimed to evaluate the accuracy of a popular smartwatch, the Huawei Watch GT2, in measuring step count (SC), total daily activity energy expenditure (TDAEE), and total sleep time (TST) during daily activities among Chinese adults, and test whether there are population differences.

Methods
A total of 102 individuals were recruited and divided into 2 age groups: young adults (YAs) and middle-aged and older (MAAO) adults. Participants’ daily activity data were collected for 1 week by wearing the Huawei Watch GT2 on their nondominant wrist and the Actigraph GT3X+ (ActiGraph) on their right hip as the reference measure. The accuracy of the GT2 was examined using the intraclass correlation coefficient (ICC), Pearson product-moment correlation coefficient (PPMCC), Bland-Altman analysis, mean percentage error, and mean absolute percentage error (MAPE).

Results
The GT2 demonstrated reasonable agreement with the Actigraph, as evidenced by a consistency test ICC of 0.88 (P<.001) and an MAPE of 25.77% for step measurement, an ICC of 0.75 (P<.001) and an MAPE of 33.79% for activity energy expenditure estimation, and an ICC of 0.25 (P<.001) and an MAPE of 23.29% for sleep time assessment. Bland-Altman analysis revealed that the GT2 overestimated SC and underestimated TDAEE and TST. The GT2 was better at measuring SC and TDAEE among YAs than among MAAO adults, and there was no significant difference between these 2 groups in measuring TST (P=.12).

Conclusions
The Huawei Watch GT2 demonstrates good accuracy in step counting. However, its accuracy in assessing activity energy expenditure and sleep time measurement needs further examination. The GT2 demonstrated higher accuracy in measuring SC and TDAEE in the YA group than in the MAAO group. However, the measurement errors for TST did not differ significantly between the 2 age groups. Therefore, the watch may be suitable for monitoring several key parameters (eg, SC) of daily activity, yet caution is advised for its use in research studies that require high accuracy.
Mesenchymal stem cells therapy for chronic ischemic stroke—a systematic review
Authors: Kurniawan Mohammad , Ramli Yetty , Putri Nadira Deanda , Harris Salim , Rasyid Al , Mesiano Taufik , Hidayat Rakhmad 
Published: October 2024

Abstract
Stroke represents a significant global health issue, primarily in the form of ischemic stroke. Despite the availability of therapeutic interventions, the recovery from chronic stroke, occurring 3 months post-initial stroke, poses substantial challenges. A promising avenue for post-acute stroke patients is mesenchymal stem cells (MSCs) therapy, which is derived from various sources and is globally recognized as the most utilized and extensively studied stem cell therapy. This systematic review, adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines, aims to synthesize evidence regarding the impact of MSCs therapy on patients with chronic ischemic stroke. Employing an advanced search strategy across databases such as PubMed, PubMed Central, Google Scholar, the Cochrane Central Register of Controlled Trials (CENTRAL), and ClinicalTrial.gov, a total of 70 studies were identified, with 4studies meeting the inclusion criteria. Although positive outcomes were observed in terms of efficacy and safety, certain limitations, such as small sample sizes, study heterogeneity, and the absence of placebo groups, undermine the overall strength of the evidence. It is crucial to address these limitations in future research, highlighting the importance of larger sample sizes, standardized methodologies, and comparative trials to improve the assessment of MSCs’ efficacy and safety. Moving forward, key priorities include exploring underlying mechanisms, determining optimal administration modes and dosages, and conducting comparative trials. By addressing these aspects, we can propel MSCs therapies toward greater efficacy, safety, and applicability across diverse patient populations.
Preventive effects of coixol, an active compound of adlay seed, in NGF-differentiated PC12 cells against beta-amyloid25-35-induced neurotoxicity
Authors: Chen Jan-Yow , Li Chien-Yu , Mong Mei-Chin , Yin Mei-Chin 
Published: October 2024

Abstract

Background
The health benefits of coixol, an active compound of adlay seed, have attracted certain attention. Adlay seed is often adopted in traditional Chinese medicine for the treatment of various inflammatory disorders. Thus, it is hypothesized that coixol could protect neuronal cells.

Objectives
The preventive effects of coixol against Abeta25-35-induced damage in nerve growth factor-differentiated PC12 cells were explored.

Methods
Differentiated PC12 cells were treated with coixol at 0.125 μM, 0.25 μM, 0.5 μM, 1 μM, and 2 μM for 48 h. Then, cells were further exposed to Abeta25-35 at 20 μM for 24 h.

Results
Coixol treatments at 0.25–2 μM exhibited antiapoptotic effect via increasing Bcl-2 mRNA expression, mitochondrial membrane potential, and Na+-K+ ATPase activity as well as decreasing Bax mRNA expression, caspase-3 activity, and intracellular Ca2+ release. In addition, coixol treatments at 0.25–2 μM alleviated oxidative and inflammatory responses via lowering reactive oxygen species level, increasing glutathione content, promoting the activity of glutathione peroxidase, glutathione reductase, and catalase, decreasing the generation of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and prostaglandin E2. Furthermore, coixol treatments at 0.25–2 μM diminished intracellular Ca2+ release, and restricted nuclear factor kappa B-binding activity and phosphorylation of p65 and p38. Coixol treatments at 0.5–2 μM increased protein generation of nuclear factor E2-related factor 2, and limited protein production of inducible nitric oxide synthase and receptor of advanced glycation end product.

Conclusion
Our novel findings suggested that coixol was a compelling agent against beta-amyloid peptide-induced neurotoxicity.
A preliminary study of primary retroperitoneal sarcoma at a tertiary University Hospital in Bangkok, Thailand: a retrospective observational study
Authors: Sriussadaporn Suvit , Sriussadaporn Sukanya , Pak-Art Rattaplee , Kritayakirana Kritaya , Prichayudh Supparerk , Samorn Pasurachate , Narueponjirakul Natawat , Aimsupanimitr Punthita , Uthaipaisanwong Apinan 
Published: October 2024

Abstract

Background
Retroperitoneal sarcoma (RPS) is rare and difficult to treat with a high recurrent rate. Very little data regarding primary RPS exists in Thailand.

Objectives
To study the outcome of treatment of primary RPS at a tertiary University Hospital in Bangkok, Thailand.

Methods
All patients who had RPS undergoing the first surgical resection at King Chulalongkorn Memorial Hospital from June 2003 to December 2019 were retrospectively enrolled in the study. Perioperative management, results of treatment, postoperative complications, and outcome were analyzed.

Results
Thirty-eight patients entered the study. Large abdominal mass was the most common presentation (90%). Liposarcoma was the most common histology (58%). Twenty patients (53%) had preoperative core needle biopsy and 21 (55%) underwent preoperative radiotherapy (RT). The tumor size ranged from 3 cm to 48 cm (median 22 cm). Five patients (13%) had total mass removal only while 33 (87%) had complete gross resection with ≥1 visceral organ resection. Surgical margins classified as R0, R1, and R2 were 61%, 34%, and 5%, respectively. Five patients (16%) had postoperative complications. There was no 30-day postoperative mortality. The local recurrence rate was 34%. Survival analysis revealed a 5-year overall survival rate of 37% and 5-year disease-free survival rate of 29%. The 5-year and 10-year recurrent rates were 71% and 95%, respectively. Multivariate analysis showed that preoperative radiation was the only factor reducing recurrence (19% vs. 53%, OR: 0.21, P = 0.011).

Conclusion
The preliminary study of outcome of the treatment of primary RPS at our institution showed a fair prognosis of this rare malignancy despite our aggressive surgical approaches. Preoperative radiation may help reduce recurrence in selected primary RPS patients.
Bioassays of Fumigant Toxicity and Repulsiveness of Haplophyllum tuberculatum Extracts
Authors: Sriti Jazia , Bachrouch Olfa , Msaada Kamel , Jallouli Slim , Limam Ferid 
Published: October 2024

Abstract
Food grains were severely damaged by stored goods insects. The red flour beetle Tribolium castaneum, the cigarette beetle Lasioderma serricorne and the rice weevil Sitophilus orysae are some of the most destructive pests which attack raw cereals throughout the world. In stored grain mill, fumigation with synthetic fumigants was the most economical tool for managing stored pests. Nevertheless, the application of chemical pesticides caused adverse effects on humans and environment. Chloroform, acetone, methanol and water extracts and essential oil of Haplophyllum tuberculatum aerial parts were tested for their fumigant and repellent effect against adults of the three stored products pests (T. castaneum, L. serricorne and S. oryzae). GC/MS analysis of the essential oil resulted in determination of 19 compounds. The major constituents were β-phellandrene (16.17%), trans-p-menth-2-ene-1-ol, (13.22%) and cis-p-menth-2-ene-1-ol (8.29%). Thus, it seemed that the essential oil had the highest fumigant activity against L. serricorne after 12 h of exposure compared to the two other insects with a LC50 equal to 38.59 µ.l-1 air. The best repellency was observed for the chloroform and methanol extracts against T. castaneum. The respective repellent doses RD50 values were = 0.03 and 0.04 mg.ml-1 after 24 h of exposure. However, after 24 h of exposure acetone extract seems to be more repellent with respective repellent dose values of = 0.14 mg.ml-1 (RD50) against L. serricorne. H. tuberculatum essential oil and extract proved to be effective in control pest infestations in grains and dry food products.
Antimicrobial Potential of Cedrus deodara Essential Oil to Preservative Effect for the Vegetables and Fruits
Authors: Kačániová Miroslava , Vukic Milena , Verešová Andrea , Ban Zhaojun , Elizondo-Luevano Joel H. , Čmiková Natália 
Published: October 2024

Abstract
A unique species of pine, Cedrus deodara is known for its wood oil. Its traditional therapeutic use is mainly antibacterial and anti-inflammatory. The aim of this study was to investigate the antibacterial properties of Cedrus deodara essential oil (CDEO) obtained from the crushed wood. The antimicrobial activity of CDEO was evaluated against Gram-negative (G-) bacteria which included Pseudomonas aeruginosa CCM 1595, Salmonella enterica subs. enterica CCM 3807 and Gram-positive (G+) bacteria Yersinia enterocolitica CCM 5671. Listeria monocytogenes CCM 4699, Staphylococcus aureus subs. aureus CCM 2461 and Streptococcus consellatus CCM 4043 in vitro and in situ. The best antimicrobial activity for the disc diffusion method ranged from 4.67 to 9.67 mm and the minimum inhibitory concentration ranged from 1.48 to 5.44 mg.mL-1. The most effective antimicrobial effect was found against S. aureus and L. monocytogenes. The vapour phase used showed the best antimicrobial effect against P. aeruginosa in the kiwifruit model and L. monocytogenes in the banana model at a lower CDEO concentration of 62.5 µg.L-1 and against P. aeruginosa in the potato model and Y. enterocolitica in the cucumber model at a higher CDEO concentration of 500 µg.L-1. CDEO showed good antimicrobial activity against bacteria on vegetable and fruit model and may be a new preservative for storage of vegetables and fruits.
Analysis of Inter-Primer Binding Site Retrotransposon Length Polymorphism in Selected Group of Vaccinium corymbosum
Authors: Klongová Lucia , Bilčíková Jana , Urbanová Lucia , Žiarovská Jana 
Published: October 2024

Abstract
The genetic variability in 13 selected genotypes of Vaccinium corymbosum using re-trotransposon-based iPBS markers system was characterized in this study. The four selected iPBS primers amplified 232 fragments, average 58 fragments per primer, approximately 53.16% of which were polymorphic. The polymorphism information content of iPBS marker was 0.238. The hierarchical cluster using unweighted pair group method with arithmetic mean analysis divided selected genotypes into two main groups and several subgroups. The values of the Jaccard coefficient of genetic relatedness were between 0.036–0.243. The similarity between two sample sets was 3.6%–24.3%. This study confirmed inter-PBS amplification technique as a reproducible and trustable marker system, but has proved to be an effective DNA fingerprinting technology to generate polymorphism in Vaccinium corymbosum.