bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors impacting physician use of information charted by others
Authors: Zozus Meredith N , Penning Melody , Hammond William E 
Published: December 2018

AbstractObjectivesTo identify factors impacting physician use of information charted by others.Materials and methodsA 4-round Delphi process was conducted with physicians and non-physicians publishing in the healthcare data quality literature to identify and characterize factors impacting physician use of information charted by others (other people or devices), either within or external to their organization. Factors with high average importance and reliability were categorized according to similarity of topic.ResultsThirty-nine factors were ultimately identified as impacting physician use of information charted by others. Five categories of factors included aspects of: the information source, the information itself, the information user, the information system, and aspects of healthcare as an institution. In addition, 4 themes were identified: (1) value of narrative text in providing context, (2) importance of mental models and personal heuristics in deciding whether, and how to use information, (3) loss of confidence in, and decreased use of information due to errors encountered, and (4) existence of a trust hierarchy potentially influencing information use.DiscussionFive similarly focused studies have recently probed clinician willingness to use information in decision-making. Our results mostly confirmed factors identified by prior studies, and uniquely identified aspects of the information user as important.ConclusionAccording to the participants in this study, information quality is prominent among factors impacting physician use of information charted by others. Based on this and similar studies, it appears that despite concerns about information quality, physicians use information charted by others.
Is the Refugee Health Screener a Useful Tool when Screening 14- to 18-Year-Old Refugee Adolescents for Emotional Distress?
Authors: Sarkadi Anna , Bjärtå Anna , Leiler Anna , Salari Raziye 
Published: December 2018

Abstract
The high number of asylum seekers in Sweden has highlighted the need for structured assessment tools to screen for refugee mental health problems in clinical services. We examined the utility of the Refugee Health Screener (RHS) in refugee adolescents, aged 14–18, attending routine clinical examinations or staying in group homes/refugee centres (N = 29). Participants completed a survey, including the RHS, administered through iPads in their native language. The RHS showed excellent internal consistency (α = 0.96) and correlated moderately with symptoms of post-traumatic stress disorder (r = 0.41, p = 0.025). Mean scores and prevalence rates were comparable to a study of adult refugees in Sweden. Unaccompanied refugee minors (URMs) scored significantly higher (M = 32.0, SD = 12.9) compared to youth staying with their families (M = 7.5, SD = 8.2, p &lt; 0.001, d = 2.27). Our findings confirm that the RHS can be used in the adolescent population in Sweden. These findings moreover suggest that URMs are a particularly vulnerable group with a large burden of mental health problems.
In 2015, 162,877 persons sought asylum in Sweden, 35,369 of whom were unaccompanied refugee minors (URMs) and another 35,015 children in families (Swedish Migration Agency, 2017). Most URMs (86 per cent) are boys, mainly from Afghanistan, Syria, Somalia and Eritrea, whereas children in families (accompanied refugee minors) more often come from Syria and Iraq with an equal gender distribution. During the asylum process, lasting up to 30 months, children have access to free education and health care. URMs are under the care of the social services and are assigned a legal guardian until they turn 18. Adults are entitled to acute health care and housing but cannot work and have no access to studies. If granted asylum, the person/family is assigned to a municipality that assumes responsibility for them. Thus, on top of adverse events before and during migration, the asylum and resettlement process per se involves stressors and a lack of control for refugees, which increases the risk of developing mental health problems.
DAZL is a master translational regulator of murine spermatogenesis
Authors: Li Haixin , Liang Zhuqing , Yang Jian , Wang Dan , Wang Hanben , Zhu Mengyi , Geng Baobao , Xu Eugene Yujun 
Published: December 2018

AbstractExpression of DAZ-like (DAZL) is a hallmark of vertebrate germ cells, and is essential for embryonic germ cell development and differentiation, yet the gametogenic function of DAZL has not been fully characterized and most of its in vivo direct targets remain unknown. We showed that postnatal stage-specific deletion of Dazl in mouse germ cells did not affect female fertility, but caused complete male sterility with gradual loss of spermatogonial stem cells, meiotic arrest and spermatid arrest. Using the genome-wide high-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation and mass spectrometry approach, we found that DAZL bound to a large number of testicular mRNA transcripts (at least 3008) at the 3′-untranslated region and interacted with translation proteins including poly(A) binding protein. In the absence of DAZL, polysome-associated target transcripts, but not their total transcripts, were significantly decreased, resulting in a drastic reduction of an array of spermatogenic proteins and thus developmental arrest. Thus, DAZL is a master translational regulator essential for spermatogenesis.
High-resolution CO images of the Galactic central molecular zone
Authors: Tokuyama Sekito , Oka Tomoharu , Takekawa Shunya , Iwata Yuhei , Tsujimoto Shiho , Yamada Masaya , Furusawa Maiko , Nomura Mariko 
Published: December 2018

Abstract
We performed Nyquist-sampled mapping observations of the central molecular zone of our Galaxy in the J = 1–0 lines of CO, 13CO, and C18O using the 45 m telescope at the Nobeyama Radio Observatory. The newly obtained data sets were an improvement by a factor of four in spatial resolution of the CO data previously obtained with the same telescope 22 years ago, providing the highest angular resolution CO atlas of this special area of the Galaxy. The data cover the area: −0${^{\circ}_{.}}$8 ≤ l ≤ +1${^{\circ}_{.}}$4 and −0${^{\circ}_{.}}$35 ≤ b ≤ +0${^{\circ}_{.}}$35 with a 15″ beamwidth. Total intensity ratios for CO J = 3–2/J = 1–0, 13CO/CO J = 1–0 and C18O/13CO J = 1–0, are 0.70 ± 0.06, 0.12 ± 0.01, and 0.14 ± 0.01, respectively. The high-resolution CO images show the fine structure of the molecular gas and enable us to identify a number of compact clouds with broad velocity widths, i.e., high-velocity compact clouds. We conducted a detailed comparison of our CO J = 1–0 data with the CO J = 3–2 data obtained with the James Clerk Maxwell Telescope to derive the distribution and kinematics of the highly excited gas. Three, out of four, of the previously identified high CO J = 3–2/J = 1–0 ratio areas at l = +1${^{\circ}_{.}}$3, 0${^{\circ}_{.}}$0, and −0${^{\circ}_{.}}$4 were confirmed with a higher spatial resolution. In addition to these, we identified several very compact, high CO J = 3–2/J = 1–0 spots with broad velocity widths for the first time. These are candidates for accelerated gas in the vicinity of invisible, point-like massive objects.
OCCUPATIONAL EXPOSURE TO EXTERNAL IONISING RADIATION IN TANZANIA (2011–17)
Authors: Muhogora Wilbroad Edward , Banzi Firm Paul , Ngaile Justin Emanuel , Ngoye Wilson Maliyatabu , Lema Usha Sara , Shao Didas , Chuma Furaha , Masoud Ali Omar , Kileo Abdallah , Mikidadi Salehe , Salum Salum K 
Published: December 2018

Abstract
The objectives of this paper were to evaluate the occupational radiation exposure data from 2011 to 2017 and to compare the results with status in 1996–2010 periods. The evaluation was performed in terms of annual collective effective dose, the average annual effective dose, the individual dose distribution ratio and the annual collective effective dose distribution ratio. Irrespective of work category, the results indicate that the average effective dose ranged from 0.64 to 1.55 mSv and broadly comparable to data in the previous analysis. Over seven year period, the maximum annual individual dose was 4 mSv and therefore below the dose limit of 20 mSv y−1. The impact of radiological practice on the exposed population was &lt;1.1 person.Sv. The results demonstrate satisfactory radiation protection conditions at workplaces, a situation which is mainly explained by the existing effective regulatory enforcement and improved workers’ awareness.
Insights into the genetic basis of predator-induced response inDaphnia galeata
Authors: Tams Verena , Nickel Jana Helene , Ehring Anne , Cordellier Mathilde 
Published: December 2018

AbstractPhenotypic plastic responses allow organisms to rapidly adjust when facing environmental challenges - these responses comprise morphological, behavioral but also life-history changes. Alteration of life-history traits when exposed to predation risk have been reported often in the ecological and genomic model organismDaphnia. However, the molecular basis of this response is not well understood, especially in the context of fish predation. Here, we characterized the transcriptional profiles of twoDaphnia galeataclonal lines with opposed life histories when exposed to fish kairomones. First, we conducted a differential gene expression, identifying a total of 125 candidate transcripts involved in the predator-induced response, uncovering substantial intra-specific variation. Second, we applied a gene co-expression network analysis to find clusters of tightly linked transcripts revealing the functional relations of transcripts underlying the predator-induced response. Our results showed that transcripts involved in remodeling of the cuticle, growth and digestion correlated with the response to environmental change inD. galeata. Furthermore, we used an orthology-based approach to gain functional information for transcripts lacking gene ontology (GO) information, as well as insights into the evolutionary conservation of transcripts. We could show that our candidate transcripts have orthologs in otherDaphniaspecies but almost none in other arthropods. The unique combination of methods allowed us to identify candidate transcripts, their putative functions and evolutionary history associated with predator-induced responses inDaphnia. Our study opens up to the question as to whether the same molecular signature is associated fish kairomones-mediated life-history changes in otherDaphniaspecies.
Affective flexibility without perceptual awareness
Authors: Homan Philipp , Lau H. Lee , Levy Ifat , Raio Candace M. , Bach Dominik R. , Carmel David , Schiller Daniela 
Published: December 2018

AbstractIn an ever-changing environment, survival depends on learning which stimuli represent threat, and also on updating such associations when circumstances shift. Humans can acquire physiological responses to threat-associated stimuli even when they are unaware of them, but the role of awareness in updating threat contingencies remains unknown. This complex process – generating novel responses while simultaneously suppressing learned ones – relies on distinct neural mechanisms from initial learning, and has only been shown with awareness. Can it occur unconsciously? Here we show that it can. Participants underwent classical threat conditioning to visual stimuli that were suppressed from their awareness. One of two images was paired with an electric shock; halfway through the experiment, contingencies were reversed and the shock was paired with the other image. We found that physiological responses reflected changes in stimulus-threat pairings independently of stimulus awareness, demonstrating the sophistication of unconscious affective flexibility.
The Genomic Landscape of Centromeres in Cancers
Authors: Saha Anjan K. , Mourad Mohamad , Kaplan Mark H. , Chefetz Ilana , Malek Sami N. , Buckanovich Ronald , Markovitz David M. , Contreras-Galindo Rafael 
Published: December 2018

ABSTRACTCentromere genomics remain poorly characterized in cancer, due to technologic limitations in sequencing and bioinformatics methodologies that make high-resolution delineation of centromeric loci difficult to achieve. We here leverage a highly specific and targeted rapid PCR methodology to quantitatively assess the genomic landscape of centromeres in cancer cell lines and primary tissue. PCR-based profiling of centromeres revealed widespread heterogeneity of centromeric and pericentromeric sequences in cancer cells and tissue as compared to healthy counterparts. Quantitative reductions in select centromeric core and pericentromeric markers were observed in neoplastic samples as compared to healthy counterparts. Subsequent phylogenetic analysis of a pericentromeric endogenous retrovirus amplified by PCR revealed possible gene conversion events occurring at numerous pericentromeric loci in the setting of malignancy. Our findings collectively represent the first look into centromere genetics in the setting of malignancy, providing valuable insight into the evolution and reshuffling of centromeric sequences in cancer development and progression.
ADHD Symptoms are Associated with the Modular Structure of Intrinsic Brain Networks in a Representative Sample of Healthy Adults
Authors: Hilger Kirsten , Fiebach Christian J. 
Published: December 2018

AbstractAttention-deficit/hyperactivity disorder (ADHD) is one of the most common neurodevelopmental disorders with significant and often lifelong effects on social, emotional, and cognitive functioning. Influential neurocognitive models of ADHD link behavioral symptoms to altered connections between and within functional brain networks. Here, we investigate whether network-based theories of ADHD can be generalized to understanding variations in ADHD-related behaviors within the normal (i.e., clinically unaffected) adult population. In a large and representative sample, self-rated presence of ADHD symptoms varied widely; only eight out of 291 participants scored in the clinical range. Subject-specific brain-network graphs were modeled from functional MRI resting-state data and revealed significant associations between (non-clinical) ADHD symptoms and region-specific profiles of between-module and within-module connectivity. Effects were located in brain regions associated with multiple neuronal systems including the default-mode network, the salience network, and the central executive system. Our results are consistent with network perspectives of ADHD and provide further evidence for the relevance of an appropriate information transfer between task-negative (default-mode) and task-positive brain regions. More generally, our findings support a dimensional conceptualization of ADHD and contribute to a growing understanding of cognition as an emerging property of functional brain networks.Author SummaryNeurocognitive models of ADHD link behavioral symptoms to altered connections between and within functional brain networks. We investigate whether these network-based theories of ADHD can be generalized to ADHD-related behaviors within the normal adult population. Subject-specific brain graphs were modeled from functional MRI resting-state data of a large and representative sample (N = 291). Significant associations between ADHD-related behaviors and region-specific profiles of between-module and within-module connectivity were observed in brain regions associated with multiple functional systems including the default-mode network, the salience network, and the central executive system. Our results support a dimensional conceptualization of ADHD and enforce network-based models of ADHD by providing further evidence for the relevance of an appropriate information transfer between task-negative (default-mode) and task-positive brain regions.
RNA Polymerase II Independent Recruitment of SPT6 at Transcription Start Sites in Arabidopsis
Authors: Chen Chen , Shu Jie , Li Chenlong , Thapa Raj K. , Nguyen Vi , Yu Kangfu , Yuan Zechun , Kohalmi Susanne E. , Liu Jun , Marsolais Frédéric , Huang Shangzhi , Cui Yuhai 
Published: December 2018

SummarySPT6 is a conserved transcription regulator that is generally viewed as an elongation factor. However, emerging evidence show its potential role in the control of transcription initiation at genic and intragenic promoters. Here we first present the genome-wide occupancy of Arabidopsis SPT6-like (SPT6L) and demonstrate its conserved role in facilitating RNA Polymerase II (RNAPII) occupancy across transcribed genes. Further, we show that SPT6L enrichment is shifted, unexpectedly, from gene body to the transcription starting site (TSS) when its association with RNAPII is disrupted. Finally, we demonstrate that recruitment of SPT6L starts at TSS, and then spreads to the gene body during transcription. These findings refine the mechanisms underlying SPT6L recruitment in transcription and shed light on the role of SPT6L in transcription initiation.
Activation of NIX-mediated mitophagy and replication by an interferon regulatory factor homologue of human herpesvirus
Authors: Vo Mai Tram , Smith Barbara J , Nicholas John , Choi Young Bong 
Published: December 2018

AbstractViral control of mitochondrial quality and content has emerged as an important mechanism for counteracting the host response to virus infection. Despite the knowledge of this crucial function of some viruses, little is known about how herpesviruses regulate mitochondrial homeostasis during infection. Human herpesvirus 8 (HHV-8) is an oncogenic virus causally related to AIDS-associated malignancies. Here, we found that HHV-8-encoded viral interferon regulatory factor 1 (vIRF-1) promotes mitochondrial clearance by activating mitophagy to support virus replication. Genetic interference with vIRF-1 expression or targeting to the mitochondria inhibits HHV-8 replication-induced mitophagy and leads to an accumulation of mitochondria. Moreover, vIRF-1 binds directly to a mitophagy receptor, NIX, on the mitochondria and activates NIX-mediated mitophagy to promote mitochondrial clearance. Genetic and pharmacological interruption of vIRF-1/NIX-activated mitophagy inhibits HHV-8 productive replication. In short, our findings uncover an essential role of vIRF-1 in mitophagy activation for successful lytic replication of HHV-8.
RNA sequencing identifies dysregulated circular RNAs in early-stage breast cancer
Authors: Arvinden Vittal Rangan , Magendhra Rao Arunagiri Kuha Deva , Meenakumari Balaiah , Ramanathan Priya , Sundersingh Shirley , Sridevi Velusami , Rajkumar Thangarajan , Herceg Zdenko , Mani Samson 
Published: December 2018

AbstractBackground:Breast cancer is a major cause of cancer related death in women worldwide. Molecular diagnostic markers that are detectable in early-stage breast cancer can aid in effective clinical intervention. Circular RNAs are a recently identified group of non-coding RNA with potential role in cancer development and progression. In this study, we aimed to identify circular RNAs specific for early stage breast cancer.Method:Circular RNA expression profile was analyzed in early-stage breast cancer tissues (N=5), matched normal counterparts (N=5) and absolute normal samples (N=5) by RNA-sequencing that enables a comprehensive analysis of RNA expression across the transcriptome. Two different algorithms, find_circ and DCC were used to identify the differentially expressed circular RNAs.Results:A total of 58 and 87 circular RNAs were found to be differentially expressed by find_circ and DCC algorithms, respectively, among which 26 circular RNAs were common. Hsa_circ_0001946 (CDR1-as) was found to be upregulated in early stage breast cancer along with other novel circular RNAs (hsa_circ_0008225, hsa_circ_0007766, hsa_circ_0016601). We also found that a few of the identified circular RNAs harbor microRNA binding sites which can lead to microRNA sponging activity and pre-microRNA sequences which can generate mature microRNAs. The identified circular RNAs that are differentially regulated in early stage breast cancer can be of potential diagnostic/prognostic importance.Conclusion:Circular RNA are differentially expressed in the early-stage breast cancer with potential application in early diagnosis and prognosis. The differentially expressed circular RNA can sequester microRNA and can act as microRNA precursor as well.
Identification of TTN as a novel candidate gene for atrioventricular block in a Chinese pedigree by whole-exome sequencing
Authors: Liu Guohui , Yang Ziying , Chen Weiwei , Xu Junguang , Mao Liangwei , Yu Qinlin , Guo Jian , Xu Hui , Liu Fengxia , Sun Yan , Huang Hui , Peng Zhiyu , Sun Jun , Li Wei , Yang Ping 
Published: December 2018

PurposeCardiovascular diseases are the most common cause of death globally. In which atrioventricular block (AVB) is a common disorder with genetic causes, but the responsible genes have not been fully identified yet. To determine the underlying causative genes involved in cardiac AVB, here we report a three-generation Chinese family with severe autosomal dominant cardiac AVB that has been ruled out as being caused by known genes mutations.MethodsWhole-exome sequencing was performed in five affected family members across three generations, and co-segregation analysis was validated on other members of this family.ResultsWhole-exome sequencing and subsequent co-segregation validation identified a novel germline heterozygous point missense mutation, c.49287C>A (p.N16429K), in the titin (TTN, NM_001267550.2) gene in all 5 affected family members, but not in the unaffected family members. The point mutation is predicted to be functionally deleterious by in-silico software tools. Our finding was further supported by the conservative analysis across species.ConclusionBased on this study, TTN was identified as a potential novel candidate gene for autosomal dominant AVB; this study expands the mutational spectrum of TTN gene and is the first to implicate TTN mutations as AVB disease causing in a Chinese pedigree.
Two-way assessment of key performance indicators to vaccine supply chain system in India
Authors: Chandra Dheeraj , Kumar Dinesh 
Published: December 2018

PurposeThe purpose of this paper is to identify the key performance indicators (KPIs) of vaccine supply chain of Universal Immunization Program (UIP) India, and measure their impact on vaccine supply chain performance improvement, so that results of this study can help the decision makers manage the activity and effectiveness of their resources in order to improve vaccine delivery performance and child immunization coverage.Design/methodology/approachThe 41 KPIs in terms of four dimension of the balanced scorecard (BSC), i.e. financial, customer, internal process and learning and growth have been identified and validated from the literature and expert’s opinions. Further, the impact of internal process and learning and growth performance indicators on the vaccine supply chain performance improvement have been evaluated using two-way assessment. The rankings obtained using two-way assessment are then compared to DEMATEL approach to validate the results.FindingsThe results from the analysis reveal that “Enhancement in employee work satisfaction” (LG3=10.08 percent), “Enhancement in professional vaccine supply chain managers and leaders” (LG2 = 6.70 percent) “Improvement in planning and coordination in the supply chain” (LG9 = 6.57 percent) are the three critical performance indicators having maximum impact on vaccine supply chain performance improvement. The decision makers should give priority to these performance indicators to improve delivery performance and immunization coverage.Social implicationsThis study focuses on the improvement in vaccine delivery performance, therefore, analysis and findings can be useful to government immunization programs of India to other developing nations to improve child immunization coverage.Originality/valueTo our knowledge, this paper is first to attempt to provide a direction to improve immunization coverage through vaccine supply chain performance indicators.