bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression and epigenetic profile of different CRISPR-Cas9 efficiency clones
Authors: Liu Yin , Mei Shenglin , Wang Hongxiu , Wang Fang , Wang Ying , Ni Lili , Xu Minyin , Lang Zhongping , Xu Weijie , Zhou Beiye , Wu Yue , Qian Fengmin , Guo Yinjuan , Wu Xiaocui , Wang Nuan , Gao Rongliang , Zhang Peng , Yu Fangyou 
Published: August 2019

Abstract
CRISPR/Cas9 cleavage efficiency is crucial in a genomic editing experiment. However, the molecular mechanisms that underlie this cleavage difference remain unclear. In our study, we characterized genome-wide gene expression and epigenetic features in CRISPR-Cas9 low and high clones across 3 different cell lines. We show that Cas9 expression level is relatively higher in high efficiency clones. Notably, histone mark ChIP-seq data demonstrate that differential expressed genes also have a different acetylation of histone 3 at lysine 27 (H3K27ac) level. Finally, we observed that PARVA is an important gene that can enhance CRISPR-Cas9 efficiency.
‘Motivational work’: a qualitative study of preventive health dialogues in general practice
Authors: Broholm-Jørgensen Marie , Langkilde Siff Monrad , Tjørnhøj-Thomsen Tine , Pedersen Pia Vivian 
Published: August 2019

Abstract
Background

While treatment plays an important role in general practice, primary and secondary prevention of so-called health behavior diseases has become an urgent issue of health politics as well as an important task in general practice. Through an analytical lens of ‘motivational work’, we explore how preventive health dialogues unfold in Danish general practices, with the purpose of understanding the social interactions and temporal complexities embedded in preventive health dialogues.

Methods

The empirical material in this study was obtained through 10 observations of preventive health dialogues and 18 semi-structured interviews with patients and general practitioners. The TOF pilot study in 2016 made it possible to explore preventive health dialogues between GPs and patients in general practice. The intervention addressed early detection and prevention in general practice through an approach of population-based risk stratification.

Results

We found that both GPs and patients—in an interplay—affected motivational work in the health dialogues to occur as one-way communication characterized by biomedically based guidance, information, suggestions, and advice on risk factors. Our findings illustrate a tendency among GPs to do motivational work without facilitating dialogue, concerning neither the underlying social causes of the behavior nor the patients’ possibilities to change their health behavior. We furthermore found that patients took part in reproducing the biomedical and treatment-oriented focus in the clinical encounter. Patients’ perspectives and expectations with regard to the structure and content of the health dialogues influenced whether and how GPs sought to promote and awaken patients’ motivation to change health behavior.

Conclusion

Overall, our findings point to a discrepancy between a biomedical action perspective and the temporality of prevention, with the former lessening the dialogue and interaction between GP and patient.
Clinical and mutational spectrum of 4 Chinese families with glutaric aciduria type 1
Authors: Shi Xiaorong , Shao Qiaoyan , Zheng Aidong , Xie Wenghuang 
Published: August 2019

Abstract
Background To investigate clinical presentation and molecular aspects of five patients suffered from glutaric aciduria type I (GA-I ), a rare neurometabolic disorder caused by glutaryl-CoA dehydrogenase deficiency due to GCDH gene mutations. Methods All five patients were diagnosed by elevated urinary glutaric acid and GCDH gene analysis. Low protein diet supplemented with special formula, GABA analog and L-carnitine were initialed following laboratory confirmation of diagnosis. The clinical and biochemical features were analyzed, and mutational analysis of GCDH was conducted using Sanger sequencing. Results Clinical manifestations of 5 patients varied from macrocephaly to severe encephalopathy, with notably different phenotype between siblings with the same mutations. Three members present complex heterozygous mutations, while two sisters present homozygous mutations. Among them, four mutations in GCDH were identified (c.1133C>T 、c.1244-2A>C 、c.339delT 、c.406G>T). Of these four mutations, c.1244- 2A>C was found in four patients and c.339delT and c.1133C>T has not yet been reported until now. Conclusions In 5 Chinese patients with GA1, two novel mutations of GCDH gene were identified, which may expand the mutation spectrum of GCDH gene. What we found confirm that there is no correlation between clinical phenotype and genotype in GA-I patients, and c.1244-2A>C may be mutation hotspot in Southern China.
Laryngeal mask airway reduces incidence of post-operative sore throat after thyroid surgery compared with endotracheal tube: a single-blinded randomized controlled trial
Authors: Gong Yahong , Xu Xiaohan , Wang Jin , Che Lu , Wang Weijia , Yi Jie 
Published: August 2019

Abstract
BackgroundSore throat is a remarkable complication after thyroid surgery with endotracheal tube (ETT). Many studies revealed that laryngeal mask airway (LMA) might reduce the incidence and severity of postoperative sore throat. However, little is known about the use of a flexible reinforced LMA (FLMA) in thyroid surgery. The purpose of this study was to explore the potential benefits of FLMA compared with ETT on postoperative sore throat.MethodsIn this prospective, single-blinded, randomized, controlled trial, ninety-six patients aged 20-80 years, scheduled for elective radical thyroidectomy under general anesthesia were enrolled. They were randomly divided into ETT group and FLMA group. All the included patients received total intravenous anesthesia (with propofol, fentanyl and rocuronium) and controlled mechanical ventilation during the surgery. Cuff pressure of ETT and FLMA were strictly controlled. Incidence and severity of postoperative sore throat, numbness and hoarseness at 1, 24, and 48 h after surgery was evaluated and compared between the two groups. Incidence and severity of buckling during extubation and the hemodynamic profile during intubation were also recorded and compared.ResultsThe incidence of sore throat and hoarseness was significantly lower in FLMA group than those in ETT group at 1h, 24h and 48h postoperatively, as well as the severity of sore throat. Compared to ETT group, there was a significantly lower incidence of buckling during extubation and less fluctuation of HR and BP at 1min and 3min after intubation in FLMA group.ConclusionsPostoperative sore throat and hoarseness was improved in patients undergoing thyroid surgery with FLMA when compared with ETT. The use of FLMA also achieved less buckling during extubation and better hemodynamic profiles during intubation.
Effect of topical or peribulbar analgesia on the incidence of oculocardiac reflex, postoperative pain and postoperative nausea and vomiting following vitreoretinal surgery under surgical pleth index-guided general anaesthesia.
Authors: Stasiowski Michal J , Pluta Aleksandra , Lyssek-Boron Anita , Szumera Izabela , Pieta Malgorzata , Kawka-Buszman Magdalena , Niewiadomska Ewa , Dobrowolski Dariusz , Krawczyk Lech 
Published: August 2019

Abstract
Background: Despite of the risk of postoperative intolerable pain perception (PIPP), vitreoretinal surgery (VRS) requires general anaesthesia (GA) in selected patients. Intraoperative use of opioid analgesics (OA) during GA carries the risk of postoperative nausea and vomiting (PONV). The surgical pleth index (SPI) optimises the intraoperative titration of OA. Regional anaesthesia techniques are combined with GA to minimize the intraoperative use of OA. We evaluated the benefit of preventive analgesia techniques combined with GA using SPI-guided fentanyl (FNT) administration on the incidence of PONV, oculocardiac reflex (OCR) and PIPP in patients undergoing VRS. Methods: One hundred and five patients undergoing VRS were randomly allocated to receive either GA with SPI-guided fentanyl (FNT) administration alone (GA group) or with preventive topical 2% proparacaine (topical anaesthesia (TA) group) or preoperative peribulbar block (PBB) using 0.5% bupivacaine with 2% lidocaine (PBB group). Fifteen patients were excluded due to problems with postoperative SPI measurement. Results: Preventive PBB resulted in intraoperative reduction in FNT requirement, with no influence on perioperative outcomes. Intraoperative SPI-guided FNT administration during GA resulted in OCR in 7.78%, PONV in 10% and PIPP in 13.5% of patients undergoing VRS. Intraoperative SPI-guided FNT administration blunted the perioperative effect of preventive PBB and TA in terms of the presence of PONV, OCR and PIPP.Conclusions: The utility of SPI-guided FNT administration during GA eliminated benefits of preventive analgesia with PBB and TA following VRS. We recommend using intraoperative SPI-guided FNT administration during GA to reduce the presence of OCR, PONV and PIPP following VRS. Trial registration: The trial was approved and registered by The Ethical Committee of Medical University of Silesia on 29th of September 2015, as well as the project was registered in the Clinical Trial Registry (SilesianMUKOAiIT2, NCT02973581)Key words: vitreoretineal surgery, general anaesthesia, peribulbar block, topical anaesthesia, surgical pleth index.
Patients with both cognitive impairment and solid cancer present increased insulin resistance
Authors: Gonda Kenji , Yaginuma Kenji , Rokkaku Yuichi , Horita Shoichiro , Maejima Yuko , Shimomura Kenju 
Published: August 2019

Abstract
Objective

In an aging population, an increase in elderly cancer patients with cognitive impairment is to be expected. Because cognitive impairment has a serious impact on quality of life of cancer patients, it is important to elucidate the possible association between cancer and cognitive impairment. Here, we focused on glucose metabolism as a factor that links cancer and cognitive impairment.

Results

A homeostasis model assessment was used to assess insulin resistance (HOMA-IR) and β-cell function (HOMA-B). HOMA-B showed no difference among patients with cancer, with dementia, and with both. However, there was a significant difference in HOMA-IR. In comparison with patients with only solid cancer, patients with only cognitive impairment and those with both cancer and cognitive impairment showed increased HOMA-IR value. Insulin resistance was increased in patients with cognitive impairment only and those with both cognitive impairment and solid cancer compared to cancer patients without cognitive impairment; however, β-cell function was not affected. The present data indicate that elderly cancer patients with a high HOMA-IR score may be in higher risk of developing cognitive impairment. Furthermore, early treatment to reduce insulin sensitivity may become the prevention of cognitive impairment.
Admission pattern, Clinical outcome and associated factors among patients admitted in medical intensive care unit at University of Gondar Comprehensive and Specialized Hospital, Northwest Ethiopia, 2019. A retrospective cross-sectional study.
Authors: Tesema Hailemariam Getachew , Lema Girmay Fitiwi , Mesfin Nebiyu , Fentie Demeke Yilkal , Arefayne Nurhussien Rizike 
Published: August 2019

Abstract
Objective: To determine the admission patterns, clinical outcomes and associated factors among patients admitted in medical intensive care unit (MICU).
Results: A total of 738 patients were admitted to MICU during September 2015- April 2019. Five hundred and four patients (68%) of all ICU admissions had complete data. Out of the 504 patients, 268 (53.2%) patients were females. Cardiovascular disease 182(36.1%) was the commonest categorical admission diagnosis. The overall mortality rate of the MICU was 38.7 %. In the multivariate analysis, mortality was associated with need for mechanical ventilation (AOR=5.87, 95% CI: 3.24 - 10.65) and abnormal mental status at admission (AOR = 2.8.8, 95% CI: 1.83-4.29). Patients who stayed less than four days in MICU are 5 times more likely to dies than who stayed(AOR= 5.58, 95% CI: 3.58- 8.69). In this study, the overall mortality was considerably high. Need for mechanical ventilator, length of ICU stay and mental status at admission were strongly associated with clinical outcome of patients admitted to MICU. Therefore, we recommend improving the acute critical care through the expansion of the care, supply emergency equipment’s and medications and implementation of admission protocols.
Key words: Admission, Intensive care unit, Length of stay, Mortality, Outcome
Knowledge of neonatal danger signs and associated factors among mothers who gave birth the last four months attending immunization services in Harar town public health facilities, Ethiopia
Authors: Tekulu Fissaha welay , Welay Fissaha Tekulu , Kassa Nega Assefa , Hadera Gebrekiros Aregawi , Assefa Natnael Etsay , Mengesha Meresa Berwo , Weldemariam Mulu Gebretsadik , Gebremeskel Senait Gebreslasie 
Published: August 2019

Abstract
Objective

The purpose of this study was to assess knowledge about neonatal danger signs and associated factors among mothers that gave birth in the last 4 months attending immunization services.Result

The study allowed 432 mothers to participate in the study. The knowledge score of neonatal danger signs was found [32.9% (95% CI: 28.9%, 37%)]. Mothers educated to secondary level were 4.9 times more likely to know about neonatal danger signs [(AOR=4.9, 95% CI: (1.15, 21). Similarly, mothers whose husband educated to college and above [AOR=4.95, 95% CI: (1.15, 21)], and being multipara mother [(AOR=2.59, 95% CI: (1.05, 6.6)], were significantly associated factors of good knowledge of mothers about neonatal danger signs.
The effects of a combination of high-protein diet and resistance training on short-term weight loss and long-term weight maintenance in overweight and obese Chinese people: a study protocol
Authors: Shaoyong Xu , Zhang Juan , Dong Yuxiang , Chen Ruikun , Xu Wenlei , Tan Zhijun , Gao Ling , Shang Lei 
Published: August 2019

Abstract
Background

It is very important for clinicians and dieticians to explore reasonable weight management strategies for obese people that address both short-term weight loss and subsequent weight maintenance. We hypothesized that resistance training combined with a high-protein diet would result in similar short-term weight loss but better long-term weight maintenance than either conventional low-fat diet control or high-protein diet alone.Methods/design

This is an 8-week randomized parallel controlled trial followed by a 24-week observational follow-up study. A 48-week supplementary follow-up study will be carried out if necessary. The study will be conducted between June 2019 and October 2020. The 90 overweight or obese participants will be randomly assigned to the conventional low-fat diet group, the high-protein diet group and the high-protein diet and resistance training combination group. Primary outcomes are body weight changes at week 8 and week 24 compared with the baseline level.Discussion

High-quality research on the effect of a high-protein diet combined resistance training on weight loss and weight maintenance is limited in Chinese population. Our study will provide a basis for obesity management in China, and promote the development of exercise and diet-related studies.Trials registration

ChiCTR1900023841, 14 June 2019
New Mechanistic Phosphorus Fertilizer Recommendation Model Based on Two Intrinsic Phosphorus Pools in Tropical Soils
Authors: Shuai Xiufu 
Published: August 2019

No mechanistic model for phosphorus (P) fertilizer recommendation is available for nutrient management in tropical soils. Soil intrinsic P pools, including a weakly and a tightly adsorbed P pool, were proposed to quantify actual soil available P. The goal of this study was to develop a new mechanistic P fertilizer recommendation model based on the two pools. The public data set of a corn (Zea mays L.) field experiment was used to estimate the daily corn P uptake. Seven soils were used to demonstrate the effects of P‐sorption site density (PSSD) and desorption rate of the tightly adsorbed P pool on the P fertilizer recommendation. Dynamics of soil P pools in a corn–soil system were modeled based on the transformation between the pools, the daily crop P uptake, and P fertilizer added at the beginning of planting. The weakly adsorbed P pool was required to be positive to represent no constraint of crop P nutrition. Results showed that the derived P recommendation model was a linear model of the total crop P uptake minus the two pools with three different coefficients. The weakly adsorbed P pool was readily available for crop uptake. The coefficients of the total crop P uptake and the tightly adsorbed P pool were >1.0 and <1.0, respectively, and they changed slightly with PSSD but were dependent on the desorption rate. The coefficient of the total crop P uptake showed a maximum 1.679 at the desorption rate 0.0118 d−1.Core Ideas
A P recommendation model was developed based on dynamic soil surface reaction and crop uptake.
Intrinsic weakly and tightly adsorbed P pools quantify dynamics of soil P supply.
P requirement was a linear function of crop uptake and initial values of P pools.
Model parameters were determined by soil P‐sorption site density and desorption rate.
Kearifan Lokal di Pedesaan: Kajian Praktik Budaya Religi Di Desa Nyatnyono
Authors: Adinugraha Hendri Hermawan 
Published: August 2019

One element of culture in Javanese society is belief or religion. Religion has two meanings: First, religion is a religion related to God, its teachings are revealed through prophets and revelations. The truth is absolutely inviolable based on the beliefs of the adherents. Second, religion are part of culture, to fulfill collective awareness and identity. Therefore, every society has a certain way and is different from one region to another in carrying out their beliefs. Nyatnyono village, for example, they have a firm belief held in the form of a ritual carried out on certain days inherited from their ancestors. In the context of this research, religion is not only a religion but also as a cultural phenomenon as well as local wisdom which is full of values practiced by its people. Based on this explanation, this study aims to describe religious culture as a form of local wisdom in the village of Nyatnyono in Semarang Regency. This research is a descriptive qualitative type of research. The results of this study indicate that there are many religious cultures that have been adopted into local wisdom for the people of Nyatnyono Village such as the iriban tradition, merti dusun, yasinan, nyadran (ruwahan), pengajian nuzul al-Qur’ān, syuronan, tirakatan, and so on. However, the tradition or habit that dominates in the village of Nyatnyono is the ziyarah of the tomb of Mbah Ḥasan Munadi and Mbah Ḥasan Dipuro and Mandi at Sendang Kalimah Ṭayyibah and Sendang Amanah, where it has been a hereditary culture for the Nyatnyono Village and those cultures that deliver Nyatnyono Village as one of the religious tourism icons in Semarang Regency with visitors from various regions so as to create jobs for the local community and be able to increase the Regional Revenue of Semarang Regency
Hanseníase: a visão do profissional da saúde no Discurso do Sujeito Coletivo
Authors: Gomes Michela Prestes , Pinto Maria Helena , Nardi Susilene Maria Tonelli , Paschoal Vânia Del’Arco 
Published: August 2019

Hanseníase; Assistência à saúde; Direito à saúde; Pesquisa qualitativa.
A Comparison of Injuries in Different Non-Professional Soccer Settings: Incidence Rates, Causes and Characteristics
Authors: Gebert Angela , Gerber Markus , Pühse Uwe , Gassmann Philippe , Stamm Hanspeter , Lamprecht Markus 
Published: August 2019

Background:
There is a lack of data regarding the epidemiology of soccer injuries and the particular accidents in specific non-professional soccer populations. The aim of this study was to analyse incidence, causes and characteristics of soccer injuries, taking into account different settings of organised (amateur) and non-organised soccer.

Methods:
A random sample of persons who had sustained an injury while playing soccer and reported this injury to the Swiss National Accident Insurance Fund (Suva) was retrospectively consulted. 705 injuries were analysed involving three main settings (soccer games, soccer training, non-organised soccer) and different amateur soccer leagues.

Results:
Knee injuries (p=0.01) and head injuries (p=0.005) were observed more frequently in games than in non-organised soccer. Injuries caused by contact with an opponent and foul play occurred more frequently in games than in training (p<0.001) or non-organised soccer (p≤0.001). Injury incidence was substantially higher for players of 30+/40+ leagues (18.7 injuries per 1000 hours) than for players of other leagues (1st-3rd amateur leagues: 8.5, p=0.002; 4th-5th amateur leagues: 9.4, p=0.007; female leagues: 8.2, p=0.006; junior leagues: 6.7, p<0.001).

Conclusion:
With respect to injury characteristics, causes and injury incidence, essential differences between various non-professional soccer settings exist suggesting that a more specific approach in injury prevention may generate positive effects.
Fractional Order Capacitor in First-Order and Second-Order Filter
Authors: Sengar Kanchan , Kumar Arun 
Published: August 2019

Background:
Fractional order Butterworth and Chebyshev (low-pass filter circuits, highpass
filter circuits and band-pass filters circuits) types of first and second order filter circuits have
been simulated and their transfer function are derived. The effect of change of the fractional order α
on the behavior of the circuits is investigated.

Objective:
This paper presents the use of fractional order capacitor in active filters. The expressions
for the magnitude, phase, the quality factor, the right-phase frequencies, and the half power frequencies
are derived and compared with their previous counterpart.

Methods:
The circuits have been simulated using Orcad as well as MATLAB for the different value of
α. We have developed the fractional gain and phase equations for low pass filter circuits, high pass filter
circuits and band pass filter circuits in Sallen-Key topology.

Results:
It is observed that the bandwidth increases significantly with fractional order other than unity
for the low pass as well as high pass and band pass filters.

Conclusion:
We have also seen that in the frequency domain, the magnitude and phase plots in the
stop band change nearly linearly with the fractional order. If we compare the fractional Butterworth
filters for low-pass and high-pass type with conventional filters then we find that the roll-off rate is
equal to the next higher order filter.