bluuebunny/crossref_metadata_2025_split huggingface dataset:
Autophagy regulation and its role in normal and malignant hematopoiesis
Authors: Shahrabi Saeid , Paridar Mostafa , Zeinvand‐Lorestani Marzieh , Jalili Arsalan , Zibara Kazem , Abdollahi Mohammad , Khosravi Abbas 
Published: June 2019

AbstractAutophagy, the molecular machinery of self‐eating, plays a dual role of a tumor promoter and tumor suppressor. This mechanism affects different clinical responses in cancer cells. Autophagy is targeted for treating patients resistant to chemotherapy or radiation. Limited reports investigate the significance of autophagy in cancer therapy, the regulation of hematopoietic and leukemic stem cells and leukemia formation. In the current review, the role of autophagy is discussed in various stages of hematopoiesis including quiescence, self‐renewal, and differentiation.
Future changes in five extreme precipitation indices in the lowlands of Romania
Authors: Harpa Gabriela‐Victoria , Croitoru Adina‐Eliza , Djurdjevic Vladimir , Horvath Csaba 
Published: June 2019

AbstractThis paper presents the results of a study focused on the projected changes in extreme precipitation indices in Romania during three future periods: 2021–2040, 2041–2070, and 2071–2100. We investigated changes in future climate based on historical and modelled data sets of daily precipitation. We compared the values calculated for the three future periods with those obtained for the historical reference period 1961–1990. The historical observation data recorded at 30 weather stations and data extracted from six regional climate model outputs (ALADIN53, CCLM4‐8‐17, RACMO22E, RCA4, REMO2009, and WRF331F) under moderate (RCP4.5) and pessimistic (RCP8.5) scenarios were employed. Five indices established by the Expert Team for Climate Change Detection Monitoring and Indices were analysed: consecutive dry days, consecutive wet days, heavy precipitation days, very heavy precipitation days, and annual total wet‐day precipitation. The series of indices were generated using ClimPACT2 software. Based on bias correction methods, the quality of the modelled data considerably increased, and the errors obtained for the daily precipitation varied in the range of ±0.05 to ±1.22 mm/day. The main findings revealed an increase in three of the extreme precipitation indices based on all models considered compared to the historical period. The most important increase (more than 50%) compared to the historical period was detected in the frequency of very heavy precipitation days and the annual total wet‐day precipitation. They were followed by the heavy precipitation days index. The consecutive dry days index indicated mainly no change or a slow decrease, while inconsistencies among the results of regional climate models were detected for the consecutive wet days index. For all indices except the consecutive dry days index, the ALADIN53 and WRF331F models provided the highest projected values for both scenarios. The dispersion of values obtained as model output for each location was higher for RCP8.5 than for RCP4.5.
Whole unripe plantain (Musa paradisiaca L.) as raw material for bioethanol production
Authors: Alonso‐Gómez Leonardo A , Heredia‐Olea Erick , Serna‐Saldivar Sergio O , Bello‐Pérez Luis A 
Published: June 2019

AbstractBACKGROUNDThe use of byproducts such as rejected plantain with final disposition problems and conversion processes with ‘green’ technologies are important research topics. Bioethanol production from crops with a high content of fermentable sugars is an alternative to that from traditional crops (corn and sugar cane). The aim of this work was to study the use of whole (peel and pulp) unripe plantain (WP) for bioethanol production.RESULTSLab‐scale liquefaction and saccharification of both materials released mainly three carbohydrates, glucose (9.02 mg g−1), maltose (0.45 mg g−1) and xylose (0.25 mg g−1). The WP saccharification required the use of pectinase and cellulase because of the high amounts of pectin and cellulose associated with the peel. Fermentation for 11 h produced similar ethanol concentration for both samples, but at the end of fermentation (32 h), the ethanol production was higher in the WP (58.6 mL L−1) compared with the plantain pulp (PP) (45.5 mL L−1). The theoretical ethanol yield was lower with WP (67%) than with PP (90%).CONCLUSIONWP can be an alternative raw material for bioethanol production. © 2019 Society of Chemical Industry
Amylose‐Coated Biohybrid Microgels by Phosphorylase‐Catalyzed Grafting‐From Polymerization
Authors: Gau Elisabeth , Flecken Franziska , Belthle Thomke , Ambarwati Masyitha , Loos Katja , Pich Andrij 
Published: June 2019

AbstractHerein, the synthesis of amylose‐coated, temperature‐responsive poly(N‐vinylcaprolactam) (VCL)‐based copolymer microgels by enzyme‐catalyzed grafting‐from polymerization with phosphorylase b from rabbit muscle is reported. The phosphorylase is able to recognize the oligosaccharide maltoheptaose as primer and attach glucose units from the monomer glucose‐1‐phosphate to it, thereby forming amylose chains while releasing inorganic phosphate. Therefore, to enable the phosphorylase‐catalyzed grafting‐from polymerization of glucose‐1‐phosphate from the PVCL‐based microgels, the maltoheptaose primer is covalently attached to the microgel in the first synthesis step. This is realized by adding N‐(2‐aminoethyl)methacrylamide (AEMAA) as a comonomer to the PVCL microgel to integrate primary amino groups and subsequent coupling of maltoheptaonolactone. Both the PVCL/AEMAA microgel as well as the obtained microgel–maltoheptaose construct are characterized in detail by dynamic light scattering, electrophoretic mobility measurements, IR spectroscopy, and atomic force microscopy. From the microgel–maltoheptaose construct, the grafting‐from polymerization of glucose‐1‐phosphate is performed by the addition of phosphorylase b. Atomic force microscopy images clearly demonstrate the formation of an amylose shell around the microgels. The developed amylose‐coated microgels open up promising application possibilities, for example, as colloidal scavengers, since amylose helices can serve as host molecules for inclusion of hydrophobic guest molecules.
Helicobacter pylori CagA promotes the malignant transformation of gastric mucosal epithelial cells through the dysregulation of the miR‐155/KLF4 signaling pathway
Authors: Ou Yang , Ren Haifeng , Zhao Rongrong , Song Le , Liu Zhengxia , Xu Wenting , Liu Yakun , Wang Siying 
Published: June 2019

AbstractThe Helicobacter pylori (H. pylori) cytotoxin‐associated gene A (CagA) and Krüppel‐like transcription factor (KLF4) were both closely associated with the development and progression of gastric cancer (GC). However, the nature of the interactions between CagA and KLF4 in GC development has not been elucidated. Therefore, we focused on the CagA‐mediated promotion of the malignant transformation of gastric epithelial cells. Herein, we first examined the expression of KLF4 in both human cancer and paracarcinoma tissues with or without H. pylori infection and found that KLF4 expression was significantly decreased in H. pylori‐positive GC cells compared with the H. pylori‐negative GC cells. Further functional studies revealed that the increased expression of CagA could suppress KLF4 expression and promote the malignant transformation of normal epithelial cells. Subsequently, we found that CagA could upregulate miR‐155 and further restrict the expression of downstream KLF4. More importantly, the overexpression of miR‐155 in GES‐1 promoted epithelial‐mesenchymal transition and eventually facilitated tumor growth in vivo. Overall, the identification of the CagA/miR‐155/KLF4 signaling pathway provided a new insight into the development and treatment of GC.
Fast quantitative 2D NMR for metabolomics and lipidomics: A tutorial
Authors: Martineau Estelle , Dumez Jean‐Nicolas , Giraudeau Patrick 
Published: June 2019

AbstractNuclear magnetic resonance (NMR) is a well‐known analytical technique for the analysis of complex mixtures. Its quantitative capability makes it ideally suited to metabolomics or lipidomics studies involving large sample collections of complex biological samples. To overcome the ubiquitous limitation of spectral overcrowding when recording 1D NMR spectra on such samples, the acquisition of 2D NMR spectra allows a better separation between overlapped resonances while yielding accurate quantitative data when appropriate analytical protocols are implemented. Moreover, the experiment duration can be considerably reduced by applying fast acquisition methods. Here, we describe the general workflow to acquire fast quantitative 2D NMR spectra in the “omics” context. It is illustrated on three representative and complementary experiments: UF COSY, ZF‐TOCSY with nonuniform sampling, and HSQC with nonuniform sampling. After giving some details and recommendations on how to apply this protocol, its implementation in the case of targeted and untargeted metabolomics/lipidomics studies is described.
Outcome in radiographic complete regression of unresectable head and neck cancer with carotid artery encasement from induction chemotherapy followed by concurrent chemoradiotherapy: Case reports
Authors: Ampil Federico , Takalkar Amol , Sangster Guillermo , Richards Troy 
Published: June 2019

AbstractLittle is known about the effects of non‐operative treatment for unresectable head and neck cancer with carotid artery encasement. While induction chemotherapy followed by concurrent chemoradiotherapy/sequential chemoradiotherapy is usually recommended for this disease entity, conclusive evidence regarding the benefit achieved from such therapeutic intervention remains sparse. The authors describe two cases of head and neck cancer with carotid artery encasement managed by induction chemotherapy followed by concurrent chemoradiotherapy, and review the literature. Despite the complete disappearance of the locally advanced tumor after the sequential chemoradiotherapy, recurrence and progression of tumor subsequently developed. The untoward event was believed to be due to the prolongation of the overall treatment time and the radiotherapy course. It is surmised that the continued use of sequential chemoradiotherapy in the management of head and neck cancer with carotid artery encasement can be justified, provided it is aggressive, timely, and without undue prolongations.
The crystal structure of Klebsiella pneumoniae FeoA reveals a site for protein‐protein interactions
Authors: Linkous Richard O. , Sestok Alexandrea E. , Smith Aaron T. 
Published: June 2019

AbstractIn order to establish infection, pathogenic bacteria must obtain essential nutrients such as iron. Under acidic and/or anaerobic conditions, most bacteria utilize the Feo system in order to acquire ferrous iron (Fe2+) from their host environment. The mechanism of this process, including its regulation, remains poorly understood. In this work, we have determined the crystal structure of FeoA from the nosocomial agent Klebsiella pneumoniae (KpFeoA). Our structure reveals an SH3‐like domain that mediates interactions between neighboring polypeptides via hydrophobic intercalations into a Leu‐rich surface ridge. Using docking of a small peptide corresponding to a postulated FeoB partner binding site, we demonstrate that KpFeoA can assume both “open” and “closed” conformations, controlled by binding at this Leu‐rich ridge. We propose a model in which a “C‐shaped” clamp along the FeoA surface mediates interactions with its partner protein, FeoB. These findings are the first to demonstrate atomic‐level details of FeoA‐based protein‐protein interactions and provide a framework for testing FeoA‐FeoB interactions, which could be exploited for future antibiotic developments.
Persistent precipitation extremes in the Yangtze River Valley prolonged by opportune configuration among atmospheric teleconnections
Authors: Chen Yang , Zhai Panmao , Liao Zhen , Li Lei 
Published: June 2019

AbstractThis study investigates whether and how three synoptic‐scale teleconnections, i.e. East Asia/Pacific teleconnection (EAP), Silk‐Road teleconnection (SR) and Eurasia teleconnection (EU), induce persistent precipitation extremes (PPEs) in the Yangtze River Valley. Results show that only the EAP teleconnection has the potential of independently incurring PPEs; while the other two teleconnections' influences on PPEs need to be exerted via their liaison with the EAP pattern. Cases are accordingly grouped into two subsets, i.e. single EAP‐PPEs and three teleconnection–PPEs. In both groups, the EAP teleconnection evolves following a similar pathway that poleward energy dispersion dynamically links a westward‐extended subtropical high, a deepened midlatitude trough and the Okhotsk blocking. EAP‐induced circulation anomalies enhance low‐level convergences and upper‐level divergences, convey exceptionally abundant moisture, and hence give birth to a quasi‐stationary front leading to PPEs in the Yangtze River Valley. Despite similarities, PPEs last noticeably longer in the three‐teleconnection context. The EU teleconnection‐related downstream energy dispersion at higher latitudes favours both earlier establishment and latter decay of the Okhotsk blocking. Constructed meridional flows at mid–high latitudes continuously steer migratory disturbances southeastward into the EAP trough. The SR plays a bigger role during the latter half of PPE life span (3 days after onset) via stimulating new cyclonic disturbances that largely overlap with the EAP trough spatially. Moreover, EU‐ and SR‐excited disturbances could effectively extract baroclinic and barotropic energy from local mean flows to replenish components of the EAP tripole pattern, which therefore survives longer and prolongs PPEs by several days.
Children's Literacy Experiences in Low‐Income Families: The Content of Books Matters
Authors: Luo Rufan , Tamis‐LeMonda Catherine S. , Mendelsohn Alan L. 
Published: June 2019

AbstractThe authors examined children's access to books in 153 four‐year‐olds from low‐income, U.S. ethnic‐minority families. Mothers reported on the number of books available to their children and the variety of books their children had, such as concept books about letters, numbers, and shapes and narrative books about cultural beliefs and relationships. Mothers also reported on the frequency of mother–child book‐sharing interactions. The authors coded characteristics of book‐sharing interactions from videos of mother–child sharing of a wordless book. Most children had a variety of concept books but few narrative books. Children who were later born and who had English‐speaking (vs. Spanish‐speaking) parents had a greater variety of narrative books than did their counterparts, and children living with their father and mother had a greater variety of narrative and concept books than those who did not reside with their father. The variety of narrative books predicted children's narrative contributions during book sharing through the mediator of mothers’ questions about the story. In contrast, the variety of concept books predicted children's referential contributions (e.g., “That's a tree”) through the mediator of mothers’ referential questions (e.g., “What's that?”). Household composition, home language use, and the content of books shape the early literacy experiences of children from low‐income, ethnic‐minority families.
Preparation and Characterization of Highly Flexible, Free‐Standing, Three‐Dimensional and Rough NiMOF/rGO Composite Paper Electrode for Determination of Catechol
Authors: Kıranşan Kader Dağcı 
Published: June 2019

AbstractThe flexible, free‐standing and durable highly rough nickel metal‐organic framework/reduced graphene oxide (NiMOF/rGO) composite paper electrode was prepared with a simple electrochemical deposition of NiMOF structures on the surface of the rGO paper electrode. NiMOF/rGO composite paper electrode was characterized by using scanning electron microscopy (SEM), X‐ray photoelectron spectroscopy (XPS), X‐ray diffraction (XRD), Raman spectroscopy and electrochemical impedance spectroscopy (EIS) techniques. As‐prepared NiMOF/rGO composite paper was used in the electrochemical detection of catechol (CC). The rough and flower‐like three‐dimensional (3D) NiMOF nanostructures on the rGO paper electrode surface greatly increased the catalytic performance of the paper electrode by providing a large active surface area. CC was detected on NiMOF/rGO composite paper in a very wide linear range from 0.02 to 760 μM, a low detection limit of 1.8 nM (S/N=3), with a high sensitivity of 0.158 mA μM cm−2 and very high selectivity, especially in the presence of phenol‐derived materials. Furthermore, it was determined that NiMOF/rGO composite paper also had high stability, flexibility, and durability, which may enable this material to be used in‐vivo applications.
Vorbemerkungen zur Architektur von Schulhäusern
Authors: Egger Jan 
Published: June 2019

ZusammenfassungDie Erstellung von Schulgebäuden ist ein komplexes Unterfangen, in das unterschiedliche staatliche und nichtstaatliche Akteure eingebunden sind. Typischerweise entstehen Schulgebäude in Wellen der Bildungs- und/oder Bevölkerungsexpansionen. Ihre Architekturen werden geprägt durch Fragen des Auftrags der Gebäude: Für welche Unterrichtsformen und welche Vorstellungen ,guten Unterrichts‘ wurden sie gebaut? Gleichzeitig erhebt sich die Frage ihrer Repräsentation: Wie stellen sich Schule und Gemeinde als Bauherrschaft dar? Solche Fragen prägen wesentlich den Möglichkeitsraum, in welchem Schulgebäude entstehen. Sie sollen im Folgenden skizziert werden.
Schulhäuser als Orte pädagogisch initiierter Sozialisationsprozesse – Theoretische Annäherung und Ableitung der Fragestellung
Authors: Egger Jan 
Published: June 2019

ZusammenfassungDas folgende Kapitel dient der Annäherung an den Forschungsgegenstand Schulhaus aus einer architektur- und bildungstheoretischen sowie einer methodologischen Perspektive, die konstitutionstheoretische Voraussetzungen enthält. Das Ziel ist die Ableitung und Klärung der Fragestellung über die Ausformulierung der theoretischen Annahmen, die für das Verständnis des Untersuchungsgegenstandes bestimmend sind. Zu klären ist, was die Architektur als Untersuchungs- und Analysegegenstand an Ergebnissen verspricht, was nicht über andere, zugänglichere und erprobte Daten erreicht werden könnte.
Architektur als Protokoll und Protokollierung von Architektur – Methode und Forschungsdesign
Authors: Egger Jan 
Published: June 2019

ZusammenfassungDie Herausforderungen einer empirischen Architektursoziologie sind nicht nur konstitutionstheoretischer Natur. Architekturanalysen sind auch in methodischer und analytischer Hinsicht eine ambitionierte Aufgabe. Dies liegt zum Ersten daran, dass Architektur synchron und diachron zugleich wirkt. Zum Zweiten liegt es daran, dass Architektur ein nichtsprachliches Protokoll ist.
Schule Schmitten im Kanton Freiburg – Siedlungsbau ist Schulhausbau und vice versa
Authors: Egger Jan 
Published: June 2019

ZusammenfassungDie Schulanlage Schmitten im Kanton Freiburg umfasst insgesamt fünf Schulhäuser und weitere Baukörper wie Bibliothek und Kindergartenpavillon, die fast nahtlos die architektonische Entwicklung der Schulbauten von 1841 bis in die Gegenwart abdecken. Der Fall Schmitten ist deshalb ertragreich, da sämtliche Schulgebäude seit der Einführung der öffentlichen Schule noch vorhanden sind und zugleich ein eigenständiges Schulhaus neuester Bauart diesen Überblick über den Schulhausbau komplementiert. Dies ermöglicht es, an einem Beispiel die Entwicklung der Schule anhand ihrer Architektur zu betrachten und zu dechiffrieren.
Personality predictors of creative achievement and lawbreaking behavior
Authors: Lebuda Izabela , Karwowski Maciej , Galang Adrianne John R. , Szumski Grzegorz , Firkowska-Mankiewicz Anna 
Published: June 2019

AbstractThis article investigates how Big Five personality traits are related to creative achievements and lawbreaking behavior in a large sample (N = 1669) of Polish adults. Structural equation modelling with personality modelled as a bi-factor structure demonstrated a weak, yet significant link (r = .21) between latent factors of creative achievements and lawbreaking behavior. A general factor of personality was unrelated to creative achievement, but negatively linked to lawbreaking behavior. Lawbreaking behavior was also negatively predicted by conscientiousness and agreeableness, while only openness positively predicted creative achievement. A person-centered analysis illustrated three distinct personality profiles: resilient, undercontrolled, and overcontrolled that differed in both lawbreaking behavior and creative achievements.
The associative polymer flooding: an experimental study
Authors: Abirov Rustem , Ivakhnenko Aleksandr P. , Abirov Zhandos , Eremin Nikolai A. 
Published: June 2019

AbstractThis study investigates the sandpack displacement of low viscosity oil (1.68 cP) by brine and aqueous solutions of associative polymers. Polymer flooding has been thoroughly investigated in many laboratory and field tests. Polymer flooding is one of the most widely used enhanced oil recovery (EOR) methods. The method of polymer flooding is not used for development of oil fields with gas caps, fractured reservoirs, high permeability and active bottom-water drive. In the application of polymer flooding, coefficient of oil recovery is increased by 3–10%. Hydrolyzed polymers undergo the significant thermal and chemical degradation at high temperature and salinity. In recent years, researchers have turned their attention to associative polymers. An application of associative polymers to withstand degradation in high temperature and high salinity conditions can enhance oil recovery in high heterogeneous fields. This article presents the results of studies of oil displacement by associative polymers in a two-layer core model. In laboratory studies, the core selected from a sandy reservoir of the South Turgay Basin of the Republic of Kazakhstan was used. Solutions of the following polymers were studied: hydrolyzed polyacrylamide (HPAM) and associative polymer. The physicochemical conditions of the experiments corresponded to the reservoir conditions of the sand layer of the South Turgay Basin: temperature of 82 °C and the salinity of the brine 92,000 ppm. Experiments revealed that the associative polymer is more stable in simulated reservoir conditions than the HPAM polymer. Associative polymer flooding was recommended for pilot testing at the reservoir of the South Turgay Basin.
The costs of dementia subtypes to California Medicare fee‐for‐service, 2015
Authors: Chen Yingjia , Wilson Leslie , Kornak John , Dudley R. Adams , Merrilees Jennifer , Bonasera Stephen J. , Byrne Christie M. , Lee Kirby , Chiong Winston , Miller Bruce L. , Possin Katherine L. 
Published: June 2019

AbstractIntroductionDementia is among the costliest of medical conditions, but it is not known how these costs vary by dementia subtype.MethodsThe effect of dementia diagnosis subtype on direct health care costs and utilization was estimated using 2015 California Medicare fee‐for‐service data. Potential drivers of increased costs in Lewy body dementia (LBD), in comparison to Alzheimer's disease, were tested.Results3,001,987 Medicare beneficiaries were identified, of which 8.2% had a dementia diagnosis. Unspecified dementia was the most common diagnostic category (59.6%), followed by Alzheimer's disease (23.2%). LBD was the costliest subtype to Medicare, on average, followed by vascular dementia. The higher costs in LBD were explained in part by falls, urinary incontinence or infection, depression, anxiety, dehydration, and delirium.DiscussionDementia subtype is an important predictor of health care costs. Earlier identification and targeted treatment might mitigate the costs associated with co‐occurring conditions in LBD.