bluuebunny/crossref_metadata_2025_split huggingface dataset:
A multicentre study to predict neonatal survival according to lung‐to‐head ratio and liver herniation in fetuses with left congenital diaphragmatic hernia (CDH): Hidden mortality from the Latin American CDH Study Group Registry
Authors: Cruz‐Martínez Rogelio , Etchegaray Adolfo , Molina‐Giraldo Saulo , Nieto‐Castro Belen , Gil Guevara Enrique , Bustillos Joaquin , Martínez‐Rodríguez Miguel , Gámez‐Varela Alma , Saldivar‐Rodríguez Daniel , Chávez‐González Erendira , Keller Rodolfo , Russo Ricardo , Yepez‐García Eduardo , Coronel‐Cruz Fausto , Torres‐Torres Johnatan , Rojas‐Macedo Alejandro , Ibarra‐Ríos Daniel , Ordorica‐Flores Ricardo , Nieto‐Zermeño Jaime , Alcocer‐Alcocer Manuel ,   on behalf of the Latin American CDH Study Group
Published: April 2019

AbstractObjectiveTo evaluate natural history of fetuses congenital diaphragmatic hernia (CDH) prenatally diagnosed in countries where termination of pregnancy is not legally allowed and to predict neonatal survival according to lung area and liver herniation.MethodsProspective study including antenatally diagnosed CDH cases managed expectantly during pregnancy in six tertiary Latin American centres. The contribution of the observed/expected lung‐to‐head ratio (O/E‐LHR) and liver herniation in predicting neonatal survival was assessed.ResultsFrom the total population of 380 CDH cases, 144 isolated fetuses were selected showing an overall survival rate of 31.9% (46/144). Survivors showed significantly higher O/E‐LHR (56.5% vs 34.9%; P < .001), lower proportion of liver herniation (34.8% vs 80.6%, P < .001), and higher gestational age at birth (37.8 vs 36.2 weeks, P < 0.01) than nonsurvivors. Fetuses with an O/E‐LHR less than 35% showed a 3.4% of survival; those with an O/E‐LHR between 35% and 45% showed 28% of survival with liver up and 50% with liver down; those with an O/E‐LHR greater than 45% showed 50% of survival rate with liver up and 76.9% with liver down.ConclusionsNeonatal mortality in CDH is higher in Latin American countries. The category of lung hypoplasia should be classified according to the survival rates in our Latin American CDH registry
Fetal anogenital distance using ultrasound
Authors: Aydin Ezra , Holt Rosemary , Chaplin Daren , Hawkes Rebecca , Allison Carrie , Hackett Gerald , Austin Topun , Tsompanidis Alex , Gabis Lidia , Ziv Shimrit Ilana , Baron‐Cohen Simon 
Published: April 2019

AbstractObjectiveThis study measured anogenital distance (AGD) during late second/early third trimester of pregnancy to confirm previous findings that AGD can be measured noninvasively in the fetus using ultrasound and further showed differences in reference ranges between populations.MethodTwo hundred ten singleton pregnancies were recruited at the Rosie Hospital, Cambridge, UK. A 2D ultrasound was performed between 26 and 30 weeks of pregnancy. AGD was measured from the centre of the anus to the base of the scrotum in males and to the posterior convergence of the fourchette in females.ResultsA significant difference in AGD between males and females (P < .0001) was found, replicating previous results with a significant correlation between estimated fetal weight (EFW) and AGD in males only (P = .006). A comparison of AGD using reference data from an Israeli sample (n = 118) and our UK sample (n = 208) showed a significant difference (P < .0001) in both males and females, after controlling for gestational age (GA).ConclusionOur results confirm that AGD measurement in utero using ultrasound is feasible. In addition, there are strong sex differences, consistent with previous suggestions that AGD is influenced by prenatal androgen exposure. AGD lengths differ between the UK and Israel; therefore, population‐specific normative values may be required for accurate clinical assessments.
Neurodevelopmental outcome following prenatal diagnosis of a short corpus callosum
Authors: Meidan Roni , Bar‐Yosef Omer , Ashkenazi Itay , Yahal Orr , Berkenstadt Michal , Hoffman Chen , Tsur Abraham , Achiron Reuven , Katorza Eldad 
Published: April 2019

AbstractObjectiveData regarding the neurodevelopmental outcome of fetal short corpus callosum (CC) diagnosed according to standard reference charts is scarce. The purpose of this study was to assess whether the finding is related to neurodevelopmental delay, and to examine reclassification to normal fetal CC length using CC length/EFW ratio.MethodHistorical prospective cohort study including pregnant women who were referred for fetal neurosonogram due to abnormal CC. Short CC was defined below the 5th percentile according to reference charts. Twenty cases were included in the study group and compared with a control group of 59 normal cases. The patients in the study group were divided into two groups according to CC length/EFW ratio. Children's neurodevelopment was assessed using the Vineland Adaptive Behavior Scale (VABS).ResultsVABS scores were within normal range in 90% of the cases. There was no significant statistical difference between the study group and the control group. In addition, there was no statistically significant difference between fetuses reclassified as normal callosal length according to CC length/EFW ratio in comparison to the control group.ConclusionThe neurodevelopmental outcome of fetuses with diagnosed short CC did not differ from the neurodevelopment of normal fetuses in the control group.
Understanding the effects of backbone chemistry and anion type on the structure and thermal behaviors of imidazolium polyimide‐ionenes
Authors: O'Harra Kathryn E , Kammakakam Irshad , Bara Jason E , Jackson Enrique M 
Published: April 2019

AbstractAdvancements in the performance and properties of ionenes can be achieved via rational molecular design strategies which combine structural elements of ionic liquids (ILs) and high‐performance polymers. The use of imidazole‐amine molecules with asymmetric reactivity has enabled the synthesis of new bis(imidazole) diimide monomers which are then polymerized via the Menshutkin reaction, followed by anion exchange to various molecular species well known in the IL literature. In this work, three types of imidazolium polyimide‐ionene backbones were synthesized starting from 1‐(3‐aminopropyl)imidazole and pyromellitic dianhydride (PMDA) or 4,4′‐(hexafluoroisopropylidene)diphthalic anhydride (6FDA) or from 1‐(4‐aminophenyl)imidazole and 6FDA, with these monomers then reacted with para‐dichloroxylene. The Cl− anions on the resultant ionenes were then exchanged with one of six molecular anions yielding a total of 18 distinct polymer compositions. The functional groups present within the cationic backbone as well as the anion type were observed to strongly influence both the thermal and organizational properties of these new ionenes. © 2019 Society of Chemical Industry
Electrospun microporous gelatin–polycaprolactone blend tubular scaffold as a potential vascular biomaterial
Authors: Joy Jincy , Pereira Jessica , Aid‐Launais Rachida , Pavon‐Djavid Graciela , Ray Alok R , Letourneur Didier , Meddahi‐Pellé Anne , Gupta Bhuvanesh 
Published: April 2019

AbstractThe work reported involved the fabrication of an electrospun tubular conduit of a gelatin and polycaprolactone (PCL) blend as an adventitia‐equivalent construct. Gelatin was included as the matrix for increased biocompatibility with the addition of PCL for durability. This is contrary to most of the literature available for biomaterials based on blends of gelatin and PCL where PCL is the major matrix. The work includes the assiduous selection of key electrospinning parameters to obtain smooth bead‐free fibres with a narrow distribution of pore size and fibre diameter. Few reports elucidate the optimization of all electrospinning parameters to fabricate tubular conduits with a focus on obtaining homogeneous pores and fibres. This stepwise investigation would be unique for the fabrication of gelatin–PCL electrospun tubular constructs. The fabricated microfibrous gelatin–PCL constructs had pores of size ca 50–100 μm reportedly conducive for cell infiltration. The measured value of surface roughness of 57.99 ± 17.4 nm is reported to be favourable for protein adhesion and cell adhesion. The elastic modulus was observed to be similar to that of the tunica adventitia of the native artery. Preliminary in vitro and in vivo biocompatibility tests suggest safe applicability as a biomaterial. Minimal cytotoxicity was observed using MTT assay. Subcutaneous implantation of the scaffold demonstrated acute inflammation which decreased by day 15. The findings of this study could enable the fabrication of smooth bead‐free microfibrous gelatin–PCL tubular construct as viable biomaterial which can be included in a bilayer or a trilayer scaffold for vascular tissue engineering. © 2019 Society of Chemical Industry
Improved Methodology for Sensitive and Rapid Quantitative Proteomic Analysis of Adult‐Derived Mouse Microglia: Application to a Novel In Vitro Mouse Microglial Cell Model
Authors: Guergues Jennifer , Zhang Ping , Liu Bin , Stevens Stanley M. 
Published: April 2019

AbstractMicroglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic function and disease‐related neuroimmune responses in the adult brain. Recently, an adult mouse brain‐derived microglial cell line has been established; however, a comprehensive proteome dataset remains lacking. Here, an optimization method for sensitive and rapid quantitative proteomic analysis of microglia is described that involves suspension trapping (S‐Trap) for efficient and reproducible protein extraction from a limited number of microglial cells expected from an adult mouse brain (≈300 000). Using a 2‐h gradient on a 75‐cm UPLC column with a modified data dependent acquisition method on a hybrid quadrupole‐Orbitrap mass spectrometer, 4855 total proteins have been identified where 4698 of which are quantifiable by label‐free quantitation with a median and average coefficient of variation (CV) of 6.7% and 10.6%, respectively. This dataset highlights the high depth of proteome coverage and related quantitation precision of the adult‐derived microglial proteome including proteins associated with several key pathways related to immune response. Data are available via ProteomeXchange with identifier PXD012006.
Effects of reallocating sedentary time with physical activity on quality of life indicators in breast cancer survivors
Authors: Welch Whitney A. , Ehlers Diane , Gavin Kara L. , Aguinaga Susan , Cottrell Alison , Nielsen Anne , Solk Payton , McAuley Edward , Phillips Siobhan M. 
Published: April 2019

AbstractObjectivesPhysical activity is associated with better quality of life (QOL) among breast cancer survivors. However, it is unknown the extent to which time spent sedentary or replacing this time with active behaviors may affect QOL. Our aim was to determine the effect of substituting time between sedentary and active behaviors on QOL indicators in breast cancer survivors.MethodsAn isotemporal substitution approach was used to examine the associations of reallocating time to sedentary and active behaviors measured by accelerometry with Functional Assessment of Cancer Treatment—Breast (FACT‐B; total, physical, social, emotional, functional well‐being, and breast cancer–specific concerns) and the Hospital Anxiety and Depression Scale (HADS) scores in a pooled analysis of breast cancer survivors (n = 753; Mage = 56.9 ± 9.5 y) from two observational studies.ResultsReallocating 30 minutes of sedentary time to 30 minutes of moderate‐to‐vigorous intensity physical activity (MVPA) was associated with improved FACT‐B total (B = 3.0; 95% CI, 0.6‐4.5), physical well‐being (B = 0.8; 95% CI, 0.33‐1.2), and functional well‐being (B = 0.6; 95% CI, 0.03‐1.2) scores. Reallocating 30 minutes of light activity to 30 minutes of MVPA was associated with improved FACT‐B total (B = 2.4; 95% CI, 0.3‐6.0) and physical well‐being (B = 0.72; 95% CI, 0.27‐1.2) scores. There was no significant substitution of time effects on HADS scores.ConclusionsSubstituting sedentary time with MVPA showed the greatest range of effects across QOL indicators. These results can inform intervention development interventions and more comprehensive activity recommendations for breast cancer survivors.
Cognitive Behavioral Stress Management intervention in Mexican colorectal cancer patients: Pilot study
Authors: Acevedo‐Ibarra Jessica Noemí , Juárez‐García Dehisy Marisol , Espinoza‐Velazco Absalón , Buenaventura‐Cisneros Sergio 
Published: April 2019

AbstractObjectiveDetermine the feasibility and the preliminary effects of brief cognitive behavioral stress management (CBSM) intervention, compared with psychoeducation (PE) group in posttraumatic stress, distress, optimism, and quality of life in patients with colorectal cancer.MethodsNinety‐four patients were randomized: 40 in CBSM intervention and 54 in PE. They answered psychological questionnaires before and after the intervention and 3 months later for follow‐up.ResultsOf the included participants, 23.40% (22) completed the sessions (11 in each group). A significant effect was obtained in the variable of optimism F2,28 = 4.0, P = 0.03, ƞ2p = 0.22 and a significant interaction effect between the groups in the avoidance behavior F2,28 = 6.0, P = 0.01, ƞ2p = 0.30 and hyperactivation F2,30 = 3.91, P = 0.03, ƞ2p = 0.20, with a large effect size in both. A significant interaction effect was found in the quality of life symptoms subscale for patients with colorectal cancer with a stoma F3,20 = 21.8, P = 0.00, ƞ2p = 0.68.ConclusionsThe CBSM intervention and PE are feasible therapies, albeit with some modifications. Preliminary effects are observed in the dimensions of posttraumatic stress and symptoms of quality of life.
Reversible control of enzyme‐inhibitor interactions with light illumination using a photoresponsive surfactant
Authors: Mirarefi Panteha , Ted Lee C. 
Published: April 2019

AbstractThe effects of a photoresponsive surfactant and light illumination on the complex formed between ribonuclease A (RNase A) and a protein ribonuclease inhibitor (RI) have been investigated to develop a light‐based technique to reactivate an enzyme through surfactant‐induced dissociation of the enzyme‐inhibitor complex. The photoresponsive surfactant undergoes a photoisomerization from the relatively hydrophobic trans isomer under visible light to the relatively hydrophilic cis isomer upon UV illumination, providing a means to reversibly control protein‐inhibitor interactions. In the absence of surfactant, RI binds tightly to RNase A through noncovalent interactions, which inhibits the enzyme activity. Upon addition of the surfactant under visible light, RNase A is reactivated, regaining ~75% of the native activity in the absence of RI. In the presence of the surfactant under UV light, however, the enzyme remains inhibited. Fluorescence spectroscopy, dynamic light scattering, and circular dichroism spectroscopy reveal that RI dramatically unfolds upon addition of the trans form of the surfactant, while RNase A does not undergo noticeable structural changes under the same conditions. This indicates that RNase A reactivation occurs through dissociation of the enzyme‐inhibitor complex arising from surfactant‐induced unfolding of the inhibitor. As a result, photoresponsive surfactant and light illumination can be used as a novel light‐based technique to dissociate enzyme‐inhibitor complexes and, thus, reactivate an inhibited enzyme.
A Ti(III) reduction method for one‐step conversion of seawater and freshwater nitrate into N2O for stable isotopic analysis of 15N/14N, 18O/16O and 17O/16O
Authors: Altabet Mark A. , Wassenaar Leonard I. , Douence Cedric , Roy Rupsa 
Published: April 2019

RationaleThe nitrogen and oxygen (δ15N, δ18O, and δ17O values) isotopic compositions of nitrate (NO3−) are crucial tracers of nutrient nitrogen (N) sources and dynamics in aquatic systems. Current methods such as bacterial denitrification or Cd‐azide reduction require laborious multi‐step conversions or toxic chemicals to reduce NO3− to N2O for 15N and 18O isotopic analyses by isotope ratio mass spectrometry (IRMS). Furthermore, the 17O composition of N2O cannot be directly disentangled using IRMS because 17O contributes to mass 45 (15N).MethodsWe describe a new one‐step chemical conversion method that employs Ti(III) chloride to reduce nitrate to N2O gas in septum sample vials. Sample preparation takes only a few minutes followed by a 24‐h reaction producing N2O gas (65–75% recovery) which partitions into the headspace. The N2O headspace was measured for 15N, 18O and 17O by IRMS or laser spectrometry.ResultsIRMS and laser spectrometric analyses gave accurate and reproducible N and O isotopic results down to 50 ppb (3.5 μM) NO3‐N, similar in precision to the denitrifier and Cd‐azide methods. The uncertainties for dissolved nitrate reference materials (USGS32, USGS34, USGS35, IAEA‐NO3) were ±0.2‰ for δ15N values and ±0.3‰ for δ18O values using IRMS. For laser‐based N2O isotope analyses the results were similar, with an δ17O uncertainty of ±0.9‰ without any need for 15N correction.ConclusionsAdvantages of the Ti(III) reduction method are simplicity, low cost, and no requirement for toxic chemicals or anaerobic bacterial cultures. Minor corrections may be required to account for sample nitrate concentration variance and potential chemical interferences. The Ti(III) method is easily implemented into laboratories currently using N2O headspace sampling apparatus. We expect that the Ti(III) method will promulgate the use of N and O isotopes of nitrate in important studies of nutrient dynamics and pollution in a wide range of aquatic ecosystems.
Remote sensing of three‐dimensional coral reef structure enhances predictive modeling of fish assemblages
Authors: Wedding Lisa M. , Jorgensen Stacy , Lepczyk Christopher A. , Friedlander Alan M. 
Published: April 2019

AbstractLiDAR (light detection and ranging) allows for the quantification of three‐dimensional seascape structure, which is an important driver of coral reef communities. We hypothesized that three‐dimensional LiDAR‐derived covariables support more robust models of coral reef fish assemblages, compared to models using 2D environmental co variables. Predictive models of coral reef fish density, diversity, and biomass were developed using linear mixed effect models. We found that models containing combined 2D and 3D covariables outperformed models with only 3D covariables, followed by models containing only 2D covariables. Areas with greater three‐dimensional structure provide fish more refuge from predation and are crucial to identifying priority management locations that can potentially enhance reef resilience and recovery. Two‐dimensional seascape metrics alone do not adequately capture the elements of the seascape that drive reef fish assemblage characteristics, and the application of LiDAR data in this work serves to advance seascape ecology theory and practice in the third dimension.
Reestablishment of Protium cordatum (Burseraceae) based on integrative taxonomy
Authors: Damasco Gabriel , Daly Douglas C. , Vicentini Alberto , Fine Paul V.A. 
Published: April 2019

AbstractSpecies delimitation remains a challenge worldwide, but especially in biodiversity hotspots such as the Amazon. Here, we use an integrative taxonomic approach that combines data from morphology, phylogenomics, and leaf spectroscopy to clarify the species limits within the Protium heptaphyllum species complex, which includes subsp. cordatum, subsp. heptaphyllum, and subsp. ulei. Molecular phylogeny indicates that populations of subsp. cordatum do not belong to the P. heptaphyllum clade, while morphology and near‐infrared spectroscopy data provide additional support for the recognition of a separate taxon. Protium cordatum (Burseraceae) is reinstated at species rank and described in detail. As circumscribed here, P. cordatum is endemic to white‐sand savannas located in the Faro and Tucuruí Districts, Pará State, Brazil, whereas P. heptaphyllum is a dominant and widespread plant lineage found in Amazonia, the Cerrado, and the Brazilian Atlantic Forest. We present an identification key to P. cordatum and closely related lineages and a detailed taxonomic description of P. cordatum, including habitat and distribution, a list and images of diagnostic features. This study demonstrates the importance of using multiple tools to characterize and distinguish plant species in highly diverse tropical regions.
CRISPR/Cas9-mediated engineering of Escherichia coli for n-butanol production from xylose in defined medium
Authors: Abdelaal Ali Samy , Jawed Kamran , Yazdani Syed Shams 
Published: April 2019

Abstract
Butanol production from agricultural residues is the most promising alternative for fossil fuels. To reach the economic viability of biobutanol production, both glucose and xylose should be utilized and converted into butanol. Here, we engineered a dual-operon-based synthetic pathway in the genome of E. coli MG1655 to produce n-butanol using CRISPR/Cas9 technology. Further deletion of competing pathway followed by fed-batch cultivation of the engineered strain in a bioreactor with glucose-containing complex medium yielded 5.4 g/L n-butanol along with pyruvate as major co-product, indicating a redox imbalance. To ferment xylose into butanol in redox-balanced manner, we selected SSK42, an ethanologenic E. coli strain engineered and evolved in our laboratory to produce ethanol from xylose, for integrating synthetic butanol cassette in its genome via CRISPR/Cas9 after deleting the gene responsible for endogenous ethanol production. The engineered plasmid- and marker-free strain, ASA02, produced 4.32 g/L butanol in fed-batch fermentation in completely defined AM1–xylose medium.