bluuebunny/crossref_metadata_2025_split huggingface dataset:
Signaling game approach to improve the MAC protocol in the underwater wireless sensor networks
Authors: Jouhari Mohammed , Ibrahimi Khalil , Tembine Hamidou , Benattou Mohammed , Ben Othman Jalel 
Published: June 2019

SummaryUnderwater sensor networks (UWSNs) are instructed for critical applications like military surveillance and underwater oil spills that conducted in a very massive three‐dimensional (3‐D) space that needs many underwater nodes (UNs) to cover the target area. Those UNs are not easy to recharge and cannot exploit solar power. MAC protocols deployed for UWSN ought to consider the energy efficiency, so as, to extend the network lifetime with total connectivity and significant throughput. Terrestrial MAC protocols could not be used for UWSN due to long and unpredictable propagation delay. Consequently, the development of a new MAC protocol for the harsh environment as underwater is a challenging task. In this study, we focus on the deployment of TDMA in UWSN for this, two schemes entitled TDMA slot sharing (TSS) and free time slots reallocation (FTSR) are proposed. Received data stored in the buffer waiting for processing and forwarding might lead to an unlimited data transfer latency those results in the buffer overflow. Otherwise, free time slots appearing during the communication process resulting from dead nodes increase uselessly sleep time for the rest of the nodes. Both schemes based on signaling game are proposed to overcome those problems, TSS is used to enable the slot sharing between UNs during the communication process to reduce the buffer overflow. FTSR scheme aims to increase the throughput of UNs by allowing the reuse of free time slots. Numerical results conducted in this work show good improvement in the network performance concerning throughput.
Salt‐tolerant native plants have greater responses to other environments when compared to salt‐tolerant invasive plants
Authors: Liu Muxin , Liao Huixuan , Peng Shaolin 
Published: June 2019

Abstract

The strong expansion potential of invasive plants is often attributed to fast adaptive responses to stress. However, the evolution of tolerance to one stressor may affect the responses to other stressors. Currently, it remains unclear what effect the evolution to one stressor might have on the responses to other single or combined stressors. Moreover, it is unknown how this might differ between invasive and native species.

Invasive plants (Mikania micrantha and Bidens pilosa) and native plants (Merremia hederacea and Sida acuta) from low‐ and high‐salinity habitats were grown under control and stressful conditions [salt stress, water stress (drought/waterlogging), and their combinations]. We explored the effects of evolved salt tolerance on the responses to water stress/combined stresses and the underlying trait mechanisms.

The high‐salinity populations of all species exhibited stronger salt tolerance than the low‐salinity populations. As to the tolerance to other stressors, the high‐salinity and low‐salinity populations of the invasive species were similar, whereas the high‐salinity populations of the native species exhibited stronger tolerance than the low‐salinity populations under most stress treatments. However, the enhanced salt tolerance in native species was accompanied by reduced total biomass under control condition. The stress tolerance of native species correlated with leaf production rate and allocation to root, while the performance of native species under control condition correlated with leaf morphology and carbon assimilation rate. This suggests a trade‐off between salt tolerance and performance in the native but not the invasive species, probably resulting from altered phenotypic/physiological traits.

SynthesisOur work suggests that the evolution of tolerance to one stressor may have stronger effects on the tolerance to other stressors of the native compared with the invasive species. This may be a new paradigm to explain the greater advantage of invasive vs. native species in highly stressful habitats.
MS‐based strategies for identification of protein SUMOylation modification
Authors: Sheng Zenghua , Wang Xixi , Ma Yanni , Zhang Dan , Yang Yanfang , Zhang Peng , Zhu Hongxia , Xu Ningzhi , Liang Shufang 
Published: June 2019

AbstractProtein SUMOylation modification conjugated with small ubiquitin‐like modifiers (SUMOs) is one kind of PTMs, which exerts comprehensive roles in cellular functions, including gene expression regulation, DNA repair, intracellular transport, stress responses, and tumorigenesis. With the development of the peptide enrichment approaches and MS technology, more than 6000 SUMOylated proteins and about 40 000 SUMO acceptor sites have been identified. In this review, we summarize several popular approaches that have been developed for the identification of SUMOylated proteins in human cells, and further compare their technical advantages and disadvantages. And we also introduce identification approaches of target proteins which are co‐modified by both SUMOylation and ubiquitylation. We highlight the emerging trends in the SUMOylation field as well. Especially, the advent of the clustered regularly interspaced short palindromic repeats/ Cas9 technique will facilitate the development of MS for SUMOylation identification.
Angler Attitudes Explain Disparate Behavioral Reactions to Fishery Regulations
Authors: Murphy Robert , Scyphers Steven , Gray Steven , H Grabowski Jonathan 
Published: June 2019

Abstract
Recreational fishery management poses unique challenges as diverse user groups often maintain disparate attitudes and behaviors, limiting our ability to predict how fishing mortality may change under future regulatory conditions. We surveyed Striped Bass Morone saxatilis anglers from multiple coastal Atlantic states to explore how potential policies may change fishing behavior, assess angler motivations and catch-related attitudes, and determine whether attitudes correlate with intended behavior. Results revealed that rule changes fundamentally changed effort allocation, whereby participants often shifted fishing effort to other saltwater species. However, disparate groups of anglers responded quite differently to potential policies. Importantly, differences in participant attitudes about fishing, such as how much they value keeping fish, were important predictors of intended behavior. Overall, our study illustrates how behavior, and thus fishing mortality, may shift under future management scenarios, and that an understanding of the characteristics of recreational fishing populations may help to predict the directionality and magnitude of these changes.
Key candidate genes of STAT1 and CXCL10 in melanoma identified by integrated bioinformatical analysis
Authors: Huang Lili , Chen Jianhua , Zhao Yu , Gu Linaer , Shao Xiaoyan , Li Jiyu , Xu Yu , Liu Zhuqing , Xu Qing 
Published: June 2019

AbstractThe underlying mechanisms and gene signatures of melanoma are unknown. In this study, three expression profile data sets (GSE65568, GSE100050, GSE114445) were integrated to identify candidate genes explaining the pathways and functions of melanoma. Expression data sets including 24 melanoma tumours and 13 normal skin samples were merged and analysed in detail. The three GSE profiles shared 431 differentially expressed genes (DEGs), including 227 upregulated genes, 200 downregulated genes and 4 differentially regulated genes. Moreover, the functions and signalling pathways of the shared DEGs with significant p‐values were identified. The two most significant modules were filtered from the DEGs protein–protein interaction (PPI) network, which consisted of 284 nodes. We also plotted the prognostic value of hub genes from an online database. In summary, using integrated bioinformatic analysis, we have identified candidate DEGs and pathways in melanoma that could improve our understanding of the causes and underlying molecular events of melanoma, and these candidate genes and pathways could be therapeutic targets for melanoma.
Development of an oral reference dose for the perfluorinated compound GenX
Authors: Thompson Chad M. , Fitch Seneca E. , Ring Caroline , Rish William , Cullen John M. , Haws Laurie C. 
Published: June 2019

AbstractAmmonium 2,3,3,3‐tetrafluoro‐2‐(heptafluoropropoxy)‐propanoate, also known as GenX, is a processing aid used in the manufacture of fluoropolymers. GenX is one of several chemistries developed as an alternative to long‐chain poly‐fluoroalkyl substances, which tend to have long clearance half‐lives and are environmentally persistent. Unlike poly‐fluoroalkyl substances, GenX has more rapid clearance, but has been detected in US and international water sources. There are currently no federal drinking water standards for GenX in the USA; therefore, we developed a non‐cancer oral reference dose (RfD) for GenX based on available repeated dose studies. The review of the available data indicate that GenX is unlikely to be genotoxic. A combination of traditional frequentist benchmark dose models and Bayesian benchmark dose models were used derive relevant points of departure from mammalian toxicity studies. In addition, deterministic and probabilistic RfD values were developed using available tools and regulatory guidance. The two approaches resulted in a narrow range of RfD values for liver lesions observed in a 2‐year bioassay in rats (0.01–0.02 mg/kg/day). The probabilistic approach resulted in the lower, i.e., more conservative RfD. The probabilistic RfD of 0.01 mg/kg/day results in a maximum contaminant level goal of 70 ppb. It is anticipated that these values, along with the hazard identification and dose‐response modeling described herein, should be informative for risk assessors and regulators interested in setting health‐protective drinking water guideline values for GenX.
Transmission of monetary policy expectations on the money markets: Comparative analysis of nontraditional monetary policy regimes in Japan
Authors: Ito Takayasu 
Published: June 2019

AbstractWhen the Bank of Japan (BOJ) adopts interest rate targeting under a comprehensive easing policy, the term structure up to 12 months in the Japanese money market is driven by a single trend. It is caused by monetary policy expectations. The regime of interest rate targeting gives a sense of comfort to market participants that the regular transmission mechanism works in the term structure of the money market. Thus, monetary policy expectations are fully transmitted to the yield curve end. On the other hand, monetary policy expectations are not fully transmitted to the yield curve end under either the quantitative and qualitative easing policy or the negative easing policy. The quantitative and qualitative easing policy and the negative interest rate policy paralyze the market function in the short‐term money market.
Long noncoding RNA AC073284.4 suppresses epithelial–mesenchymal transition by sponging miR‐18b‐5p in paclitaxel‐resistant breast cancer cells
Authors: Wang Yue‐Yue , Yan Lei , Yang Shuo , Xu He‐Nan , Chen Tian‐Tian , Dong Zheng‐Yuan , Chen Su‐Lian , Wang Wen‐Rui , Yang Qing‐Ling , Chen Chang‐Jie 
Published: June 2019

AbstractBreast cancer (BC) is the most prevalent malignant cancer in the world, is the leading cause of cancer‐related death female. Recently, there is accumulating evidence that long noncoding RNAs (lncRNAs) might as an important role in the progression of BC. (epithelial‐mesenchymal transition (EMT) is considered to play a vital role in tumor cells migration and invasion. Nevertheless, the entire biological mechanisms and functions of lncRNAs in tumor migration, invasion, and EMT remain uncertain. In the present research, we observed that the expression of lncRNA AC073284.4 was downregulated in BC paclitaxel‐resistant (PR) cells (MCF‐7/PR) and tissues. Bioinformatics analysis predicted that miR‐18b‐5p was a direct target of AC073284.4, which has been validated by dual‐luciferase reporter gene assay. We further proved that AC073284.4 could directly bind to miR‐18b‐5p and relieve the suppression for dedicator of cytokinesis protein 4 (DOCK4). Furthermore, the underlying functional experiments demonstrated that AC073284.4 might sponge miR‐18b‐5p to attenuate the invasion, metastasis, and EMT of BC cell through upregulating DOCK4 expression. In summary, AC073284.4 might serve as a competing endogenous RNA (ceRNA) in BC progression via modulating miR‐18b‐5p/DOCK4 axis, which weakens EMT and migration of BC. These results suggesting that AC073284.4 might function as a potential novel diagnostic biomarker in the progression of BC.
Potential drugs used in the antibody–drug conjugate (ADC) architecture for cancer therapy
Authors: Yaghoubi Sajad , Karimi Mohammad Hossein , Lotfinia Majid , Gharibi Tohid , Mahi‐Birjand Motahare , Kavi Esmaeil , Hosseini Fahimeh , Sineh Sepehr Koushan , Khatami Mehrdad , Bagheri Nader , Abdollahpour‐Alitappeh Meghdad 
Published: June 2019

AbstractCytotoxic small‐molecule drugs have a major influence on the fate of antibody–drug conjugates (ADCs). An ideal cytotoxic agent should be highly potent, remain stable while linked to ADCs, kill the targeted tumor cell upon internalization and release from the ADCs, and maintain its activity in multidrug‐resistant tumor cells. Lessons learned from successful and failed experiences in ADC development resulted in remarkable progress in the discovery and development of novel highly potent small molecules. A better understanding of such small‐molecule drugs is important for development of effective ADCs. The present review discusses requirements making a payload appropriate for antitumor ADCs and focuses on the main characteristics of commonly‐used cytotoxic payloads that showed acceptable results in clinical trials. In addition, the present study represents emerging trends and recent advances of payloads used in ADCs currently under clinical trials.
Mst1 deletion reduces septic cardiomyopathy via activating Parkin‐related mitophagy
Authors: Shang Xiuling , Lin Kaiyang , Zhang Yingrui , Li Min , Xu Jingqing , Chen Kaihua , Zhu Pengli , Yu Rongguo 
Published: June 2019

AbstractCardiomyocyte function and viability are highly modulated by mammalian Ste20‐like kinase 1 (Mst1)‐Hippo pathway and mitochondria. Mitophagy, a kind of mitochondrial autophagy, is a protective program to attenuate mitochondrial damage. However, the relationship between Mst1 and mitophagy in septic cardiomyopathy has not been explored. In the present study, Mst1 knockout mice were used in a lipopolysaccharide (LPS)‐induced septic cardiomyopathy model. Mitophagy activity was measured via immunofluorescence, Western blotting, and enzyme‐linked immunosorbent assay. Pathway blocker and small interfering RNA were used to perform the loss‐of‐function assay. The results demonstrated that Mst1 was rapidly increased in response to LPS stress. Knockout of Mst1 attenuated LPS‐mediated inflammation damage, reduced cardiomyocyte death, and improved cardiac function. At the molecular levels, LPS treatment activated mitochondrial damage, such as mitochondrial respiratory dysfunction, mitochondrial potential reduction, mitochondrial ATP depletion, and caspase family activation. Interestingly, in response to mitochondrial damage, Mst1 deletion activated mitophagy which attenuated LPS‐mediated mitochondrial damage. However, inhibition of mitophagy via inhibiting parkin mitophagy abolished the protective influences of Mst1 deletion on mitochondrial homeostasis and cardiomyocyte viability. Overall, our results demonstrated that septic cardiomyopathy is linked to Mst1 upregulation which is followed by a drop in the protective mitophagy.
The cytokine network type I IFN-IL-27-IL-10 is augmented in murine and human lupus
Authors: Lee Michael H , Gallo Paul M , Hooper Kirsten M , Corradetti Chelsea , Ganea Doina , Caricchio Roberto , Gallucci Stefania 
Published: June 2019

Abstract
IL-10 is elevated in the autoimmune disease systemic lupus erythematosus (SLE). Here, we show that conventional dendritic cells (cDCs) from predisease lupus-prone B6.NZM Sle1/Sle2/Sle3 triple congenic (TCSle) mice produce more IL-10 than wild-type congenic cDCs upon TLR stimulation, and this overproduction is prevented by blocking the type I IFN receptor (IFNAR) with specific Abs. Priming wild-type cDCs with type I IFN mimics the IL-10 overproduction of TCSle cDCs. The MAPK ERK is more phosphorylated in lupus cDCs, partially contributing to IL-10 overproduction. Moreover, we found that TCSle cDCs express higher levels of IL-27 upon TLR7/TLR9 stimulation, and IFNAR blockade reduced IL-27 levels in TCSle cDCs. These results suggest that dysregulated type I IFNs in cDCs contribute to the increased IL-10 and IL-27 in SLE. Since IL-27 neutralization did not inhibit TLR-induced IL-10 production, we propose that type I IFNs enhanced IL-10 in TCSle cDCs independently from IL-27. Moreover, RNA sequencing analysis of a cohort of SLE patients reveals higher gene expression of these cytokines in SLE patients expressing a high IFN signature. Since IL-27 and IL-10 have both pro- and anti-inflammatory effects, our results also suggest that these cytokines can be modulated by the therapeutic IFN blockade in trials in SLE patients and have complex effects on the autoimmune response.
Domino liver transplantation for select metabolic disorders: Expanding the living donor pool
Authors: Celik Neslihan , Squires James E. , Soltys Kyle , Vockley Jerry , Shellmer Diana A. , Chang Wonbae , Strauss Kevin , McKiernan Patrick , Ganoza Armando , Sindhi Rakesh , Bond Geoffrey , Mazariegos George , Khanna Ajai 
Published: June 2019

AbstractDomino liver transplantation (DLT) involves transplanting liver from a patient with metabolic disease into a patient with end‐stage liver disease with the expectation that the recipient will not develop the metabolic syndrome or the recurrent syndrome will have minimal affect. The domino donor gets a deceased donor or a segment of live‐donor liver through the deceased donor organ allocation system. Waitlist mortality for the domino recipient exceeds morbidity associated with getting the donor disease. Between 2015 and 2017, four patients with three metabolic disorders at UPMC Children's Hospital of Pittsburgh underwent DLT with domino allografts from maple syrup urine disease (MSUD) patients. These included patients with propionic acidemia (PA) (n = 1), Crigler‐Najjar (CN) syndrome type‐1 (n = 2), and carbamoyl phosphate synthetase deficiency (CPSD) (n = 1). Mean follow‐up was 1.6 years (range 1.1‐2.1 years). Total bilirubin levels normalized postoperatively in both CN patients and they maintain normal allograft function. The PA patient had normal to minimal elevations of isoleucine and leucine, and no other abnormalities on low protein diet supplemented with a low methionine and valine free formula. No metabolic crises have occurred. The patient with CPSD takes normal baby food. No elevation in ammonia levels have been observed in any of the patients. DLT for a select group of metabolic diseases alleviated the recipients of their metabolic defect with minimal evidence of transferrable‐branched chain amino acid elevations or clinical MSUD despite increased protein intake. DLT using allografts with MSUD expands the live donor liver pool and should be considered for select metabolic diseases that may have a different enzymatic deficiency.
User and system pitfalls in liver imaging with LI‐RADS
Authors: Elsayes Khaled M. , Fowler Kathryn J. , Chernyak Victoria , Elmohr Mohab M. , Kielar Ania Z. , Hecht Elizabeth , Bashir Mustafa R. , Furlan Alessandro , Sirlin Claude B. 
Published: June 2019

The Liver Imaging Reporting and Data System (LI‐RADS) is a comprehensive system for standardizing the terminology, technique, interpretation, reporting, and data collection of liver imaging, created specifically for patients at risk for hepatocellular carcinoma. Over the past years, LI‐RADS has been progressively implemented into clinical practice, but pitfalls remain related to user error and inherent limitations of the system. User pitfalls include the inappropriate application of LI‐RADS to a low‐risk patient population, incorrect measurement techniques, inaccurate assumptions about LI‐RADS requirements, and improper usage of LI‐RADS terminology and categories. System pitfalls include areas of discordance with the Organ Procurement and Transplantation Network (OPTN) as well as pitfalls related to rare ancillary features. This article reviews common user pitfalls in applying LI‐RADS v2018 and how to avoid preventable errors and also highlights deficiencies of the current version of LI‐RADS and how it might be improved in the future.Level of Evidence:3Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019. J. Magn. Reson. Imaging 2019;50:1673–1686.
Agronomic biofortification of cowpea with selenium: effects of selenate and selenite applications on selenium and phytate concentrations in seeds
Authors: Silva Vinícius M , Boleta Eduardo HM , Martins Juliana T , dos Santos Flavia LM , da Rocha Silva Anne C , Alcock Thomas D , Wilson Lolita , de Sá Marco E , Young Scott D , Broadley Martin R , White Philip J , dos Reis André R 
Published: June 2019

AbstractBACKGROUNDSelenium (Se) is a nutrient for animals and humans, and is considered beneficial to higher plants. Selenium concentrations are low in most soils, which can result in a lack of Se in plants, and consequently in human diets. Phytic acid (PA) is the main storage form of phosphorus in seeds, and it is able to form insoluble complexes with essential minerals in the monogastric gut. This study aimed to establish optimal levels of Se application to cowpea, with the aim of increasing Se concentrations. The efficiency of agronomic biofortification was evaluated by the application of seven levels of Se (0, 2.5, 5, 10, 20, 40, and 60 g ha−1) from two sources (selenate and selenite) to the soil under field conditions in 2016 and 2017.RESULTSApplication of Se as selenate led to greater plant Se concentrations than application as selenite in both leaves and grains. Assuming human cowpea consumption of 54.2 g day−1, Se application of 20 g ha−1 in 2016 or 10 g ha−1 in 2017 as selenate would have provided a suitable daily intake of Se (between 20 and 55 μg day−1) for humans. Phytic acid showed no direct response to Se application.CONCLUSIONSelenate provides greater phytoavailability than selenite. The application of 10 g Se ha−1 of selenate to cowpea plants could provide sufficient seed Se to increase daily human intake by 13–14 μg d−1. © 2019 Society of Chemical Industry
Stability and stabilization of (–)‐gallocatechin gallate under various experimental conditions and analyses of its epimerization, auto‐oxidation, and degradation by LC‐MS
Authors: Wu Qian‐Qian , Liang Yan‐Fei , Ma Sheng‐Bo , Li Heng , Gao Wen‐Yun 
Published: June 2019

AbstractBACKGROUND(–)‐Gallocatechin gallate (GCG) shows multi‐bioactivities. Its stability, however, has not been investigated systematically yet. Therefore, the objective of this study was to characterize the stability of GCG and to find ways to stabilize it in biological assays. Furthermore, the epimerization of the compound, its auto‐oxidation and degradation were also analyzed by liquid chromatography mass spectrometry (LC‐MS).RESULTSThe stability of GCG was concentration‐dependent and was sensitive to pH, temperature, bivalent cations, and dissolved oxygen level. The results also showed that GCG was not stable in common buffers (50 mmol L−1, pH 7.4, 37 °C) or in cell culture medium DMEM/F12 under physiological conditions (pH 7.4, 37 °C). Our experiments indicated that nitrogen‐saturation and the addition of ascorbic acid (VC) could stabilize GCG in biological assays. In addition, LC‐MS determination indicated that GCG was able to be epimerized to its epimer (−)‐epigallocatechin gallate (EGCG). Meanwhile it was also able to be auto‐oxidized to theasinensin and compound P2 and degraded to gallocatechin and gallic acid in pure water at 100 °C.CONCLUSIONThe stability of GCG should be seriously considered in research on the bioactivity of it to avoid possible artifacts. Nitrogen‐saturation and use of VC are good ways to make GCG stable in biological assays. © 2019 Society of Chemical Industry