bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of a functional recombinant vascular endothelial growth factor 165 (VEGF165) in Arabidopsis thaliana
Authors: Qiang Weidong , Feng Xue , Li Yixin , Lan Xinxin , Ji Kun , Sun Xiaoyu , Chen Xi , Li Haiyan , Du Linna , Yang Jing 
Published: August 2018

Abstract

Objective
Targeting the protein of interest to a particular tissue to achieve high-level expression is an important strategy to increase expression efficiency. The use of the plant seed oil body as a bioreactor can not only increase the amount of target protein, but also reduce the cost of downstream processing.

Methods
VEGF165 was expressed in Arabidopsis thaliana seeds via oilbody fusion technology. The pKO-VEGF165 vector was construted and transformed into A. thaliana seeds. T3 transgenic seeds was detected by SDS-PAGE and western blot methods. The cell activity was tested by MTT methods.

Result
The phaseolin promoter was used to drive seed-specific expression of the VEGF
165

gene in transgenic A. thaliana. The coding region of VEGF
165

was fused to the Arabidopsis oleosin sequence to target the protein to the oil bodies in the seeds of transgenic plants. The T-DNA region of recombinant plasmid pKO-VEGF165 was shifted to A. thaliana seeds via the floral-dip method. Protein was analyzed by electrophoresis and protein hybridization analyses. Finally, MTT assays showed that the oleosin-VEGF165 fusion protein played a part in the proliferation of HUVEC cells in vitro.

Conclusion
Oleosin-VEGF165 was successfully expressed and it had stimulated HUVEC cell proliferation activity.
Paul, the Law and Judaism: Stoification of the Jewish Approach to the Law in Paul’s Letter to the Romans
Authors: Holtz Gudrun 
Published: August 2018

Abstract
One of the major issues in Pauline studies of the last decades has been the picture of Judaism painted by Paul especially in his Letter to the Romans. To that end, scholarship first and foremost adduced contemporary Jewish sources without, however, reaching satisfactory results. The present paper, therefore, takes a different approach in that it works out the Stoic influence on Paul’s argumentation. To begin with, the Stoic character of the dilemma of the “I” in Rom 7:14–25, that wills the good, but fails to do so, is demonstrated. Thereupon, it is shown that Paul transfers insights of Stoic psychology not only to the Jewish-Adamitic “I” but also to Israel’s approach to the law that is reached by neither one. Paul in Romans, therefore, can be said to stoify the Jewish approach to the law which especially reflects in the formula “righteousness by works of the law”.
Early Safety and Efficacy of Sapien 3 20 mm Transcatheter Heart Valve Implantation in Small Japanese Body Size
Authors: Meguro Kentaro , Kakizaki Ryota , Hashimoto Takuya , Yanagisawa Tomoyoshi , Koitabashi Toshimi , Kitamura Tadashi , Ako Junya 
Published: August 2018

Background: Transcatheter aortic valve implantation (TAVI) is effective in treating severe aortic stenosis in inoperable or high-risk surgical patients, however, the little is known about outcomes after Sapien 3 20 mm transcatheter heart valve (THV) implantation. The purpose of this study was to investigate the short term outcomes of Sapien 3 20 mm THV implantation in Japanese people with a small body size.
Methods: We retrospectively collected the hospital records of consecutive patients who underwent TAVI using the Sapien 3 20 mm THV between October 2016 and March 2017. Clinical and echocardiographic data from before and one week after TAVI were collected.
Results: Six Japanese patients (all female, mean age 89 ± 5 years, body surface area [BSA] 1.29 ± 0.16m2) received a Sapien 3 20 mm THV. All the procedures were feasible and successful, and the 30-day mortality rate was 0%. The functional class and the echocardiographic findings significantly improved (aortic valve area, 0.5 ± 0.1 cm2 to 0.8 ± 0.1cm2; mean pressure gradient, 55 ± 15 mmHg to 19 ± 7 mmHg; P = .043, respectively). However, the values of the indexed effective orifice area in all patients after Sapien 3 20 mm THV implantation were less than 0.85 cm2/m2, suggesting prosthesis-patient mismatch (PPM).
Conclusions: The implantation of a Sapien 3 20 mm THV was safe and effective in high surgical risk elderly Japanese patients with a small body size. PPM after Sapien 3 20mm THV may be prevalent among Asians with small body sizes. Careful clinical follow-up will be necessary after Sapien 3 20 mm THV implantation.
HEART CONDUCTION AND RHYTHM DISORDERS IN IKTAL EPILEPSY PERIOD: THE PREVALENCE AND PREDICTORS
Authors: Rubleva Y. V. , Serdyuk S. E. , Teryan R. А. , Davtyan K. V. , Burd S. G. , Drapkina O. M. 
Published: August 2018

Aim.To assess the characteristics and prevalence of cardiac rhythm disorders in epilepsy patients during the iktal period, by long term ECG monitoring.Material and methods.To the study, 79 patients included, age 18 to 51 y. o. with confirmed diagnosis of focal epilepsy with continuing attacks regardless the antiepilepsy treatment. For rhythm disorder screening, all patients underwent implanting of subcutaneous ECG monitors.Results.Disorders of cardiac rhythm and conduction during the iktal period were found in 88,61% cases. Most prevalent were sinus tachycardia (59,5%, 47 cases), cardiac rhythm and conduction disorders (29%, 23 cases). In 9 cases (11,4%) there were no cardiac rhythm and conduction disorders registered. Also, the probable predictors found, of the disorders of cardiac rhythm in epilepsy patients. Patients gender, type of attacks, frequency, circadian specifics were the markers influencing the rate of iktal cardiac rhythm changes.Conclusion.Sinus tachycardia, rhythm and conduction disorders are registered in most of patients during the iktal period and significantly more common in males, the patients with bilateral tonic and clonic seizures, serial course of the attacks and in the patients with sleep-related seizures. Evaluation of antiepilepsy treatment influence on the prevalence of iktal rhythm and conduction disorders did not reveal significant difference for none of the drug groups. Also, there was no significant differences in comparison of the studied parameters for polyand monotherapy by anticonvulsants.
RETROSPECTIVE EFFICACY ANALYSIS OF ACUTE VASOREACTIVE TEST AS A CRITERIA FOR SURGERY IN CHILDREN WITH INBORN LEFT-TO-RIGHT BLOOD SHUNTING AND PULMONARY ARTERIAL HYPERTENSION
Authors: Mullen A. , Butrous G. , Abzaliev K. 
Published: August 2018

Aim. Retrospective analysis of efficacy of the acute vasoreactive test (AVRT) as a criteria for operability of children with inborn left-to-right blood shunting complicated by pulmonary arterial hypertension (IRLBS-PAH), by an experience of one center.Material and methods. Retrospective analysis of the data of right heart chambers catheterization and echocardiographic study from 29 case histories of BS-PAH patients during 2012-2016. Results of AVRT are interpreted by modified Barst criteria (decline of pulmonary vascular resistance index, PVRI, and relation of PVRI to the index of systemic vascular resistance, SVR more than 20%, and the and PVRI <6 Wood units/m2 in PVR/SVR <0,3). In accordance with the results of AVRT, patients were selected to two main groups: group 1 — children with positive response, group 2 — children with negative response. After correction of the inborn defect in bith groups, following assessment of cardiovascular system condition was done by echocardiographical study in one week and one month. Metrics of echocardiography data was indexed. For the defect of interventricular septum (DIVS), indexation of the size was done via the relation to aortic root diameter, that was measured in parasternal position, longitudinal axis. Systolic pressure in the right ventricle was measured by the velocity of tricuspid regurgitation, measured in apical four-chamber position. Mean values with the standard deviation and p-values were calculated in R studio 2017 software, v.1.0.153.Results. Among 29 patients, girls to boys relation was 4,8:1. Mean age 6,0±4,9 y. o. For group 1: median of DIVS 1,4 with the value of additional shunting 9,93±9,39 mm, baseline systolic pressure in the right ventricle (SPRV) 57,96±20,16 mmHg, in one month after surgery 38,96±14,16 mmHg. No complications registered. In the second group, median DIVS 0,4 with additional shunting 3,3±0 mm. Baseline SPRV 66,05±17,27 mmHg, with the decline in one month after transcatheter closure to 57,4±17,35 mmHg. During the early post-surgery period, in 2 patients of group 2 there was pulmonary crisis, treated with inhalatory iloprost. Conclusion. Acute vasoreactive test is effective criteria of operability assessment of inborn left-to-right blood shunting, complicated by pulmonary arterial hypertension, in pediatrics.
ROLE OF THE NOTCH1 GENE IN FORMATION OF AORTIC ANEURYSM
Authors: Irtyuga O. V. , Freilichman O. A. , Krivonosov D. S. , Malashicheva A. B. , Tarnovskaya S. I. , Uspenskiy V. Е. , Gordeev M. L. , Rotar O. P. , Kostareva A. A. , Moiseeva O. M. 
Published: August 2018

Aim. To evaluate the impact of genetics in development of thoracic aneurysm in patients with tricuspid valve (TAV) and bicuspid aortic valve (BAV) based on the analysis and search for mutations in NOTCH1.Material and methods. In the study, 60 patients included with the dilation of thoracic aorta more than 40 mm and 200 patients with no aortic pathology, included in the comparison group. All patients underwent echocardiographic assessment on Vivid 7 (GE, USA) equipment, by standard protocol. For molecular genetics we utilized the strategy of targeted mutation screening, including the analysis of 10 of 34 exones of the gene NOTCH1, performed with the direct sequencing.Results. Patients with BAV were younger than those with no inborn defect. Arterial hypertension was verified only in every second BAV patient. Also, maximal rates of blood pressure were significantly lower in patients with inborn defects (р<0,02). As a result of genetic analysis in the studied group, in 9 patients with inborn defect and 2 patients with TAV there were 10 variants found of aminoacid replacement in 6 among 10 analyzed exones, of those 5 replacements — synonimic, and the mutation S2449R was found first time. Mutations P1227S, E1305K, R1279H and D1267N were found at the site of Notch1 protein binding with DLL4, of those 3 are highly pathogenic, that could influence the protein-protein interactions Notch1 with DLL4 leading to formation on aneurysm.Conclusion. Mutations P1227S D1267, E1305K in the gene NOTCH1, being highly pathogenic, may lead to the changes of protein functioning via Notch signalling disorder, that is more characteristic for BAV patients.
CLINICAL CHRACTERISTICS OF PATIENTS AND RESULTS OF CATHETER ABLATION IN ATRIAL FIBRILLATION IN RUSSIA: SUBANALYSIS OF THE EUROPEAN REGISTRY 2012-2016
Authors: Mikhailov E. N. , Gasymova N. Z. , Bayramova S. А. , Kharats V. E. , Kachalkova O. N. , Dmitriev A. Yu. , Batalov R. E. , Morgunov D. P. , Silin I. A. , Aleksandrovskiy A. A. , Kryzhanovskiy D. V. , Romanov A. B. , Pokushalov E. A. , Lebedev D. S. , Kuznetsov V. A. , Kolunin G. V. , Zamanov D. A. , Chetverikov S. Yu. , Yashin S. M. , Popov S. V. , Ivanitsky E. A. , Gorkov A. I. , Mamchur S. E. , Bazaev V. A. , Shlyakhto E. V. 
Published: August 2018

Aim. The results presented, of subanalysis of the catheter ablation (CA) registry in atrial fibrillation (AF) with the patients properties, specifics of CA and treatment results, in Russia comparing to European countries.Material and methods. During 2012 to 2015, totally 3742 patients included to the registry, of those 477 in Russia. In 467 Russian patients (males 56,5%; mean age 58,5 y. o.) CA AF was done. During one year 392 patients were followed up. Minimum requirements to follow-up: routine ECG registration and non less than one contact after 12 months passed.Results. In Russian patients there were more common obesity (46,1% and 29,2%, p<0,001), hypertension heart disease (40,2% and 22,8%, p<0,0001), coronary heart disease (31,7% and 16,2%, p<0,0001), chronic heart failure (67,3% and 13,0%, p<0,0001). In Russia the patients more commonly underwent primary CA (83,5% in Russia and 77,6% in European countries, p<0,05), more rare in Russia the cryoballoon ablation was done (3% and 18%, p<0,05). Generally adverse events were reported more rare in Russia (10,5% and 16,6%, p=0,0007), including cardiovascular adverse events (2,6% and 5,2%, p<0,05). Tachiarrhythmias recurs were diagnosed more rare in Russia, including by the subsutaneous ECG monitors (17% and 1,6% in other countries, р<0,001). Within the year of follow-up, full absence of tachiarrhythmias recurs in Russia was found in 65,8% of patients, in other countries — in 74,7% (р=0,0003).Conclusion. In real clinical setting, high efficacy of CA AF was shown, resistant to antiarrhythmic therapy. In most of Russian patients there were cardiovascular comorbidities. There was lower rate of reported adverse events in Russian centers of interventional treatments.
Pharmacoeconomic analysis of using typical and atypical antipsychotics in schizophrenia
Authors: Vilyum I. A. , Andreev B. V. , Proskurin M. A. , Balykina Yu. E. 
Published: August 2018

The aim: to provide a comprehensive pharmacoeconomic evaluation of the maintenance therapy with antipsychotics in outpatients diagnosed with schizophrenia.Materials and methods. The analysis was conducted by two mutually complementary steps: an epidemiological study on outpatients with schizophrenia, and a subsequent pharmacoeconomic modeling. Two medical technologies were evaluated and compared: treatment with classical antipsychotics (kA) and treatment with atypical antipsychotics (AA). For the clinical and economic analysis of these treatments, we used a number of indices derived from our retrospective study of patients’ medical records. The cost-effectiveness analysis, incremental analysis, and «budget impact» analysis were performed taking into account the direct and indirect costs of the treatments.Results. We determined the costs of managing outpatients with schizophrenia from the perspective of the healthcare budget and the social burdens; we also looked into the relevance and effectiveness of the current costs at various time intervals – 6, 12 and 24 months. As shown, the treatment strategies involving AA were more budget-consumptive than the kA treatments. even if the treatments were switched to the reproduced AA (up to 100% replacement), the costs would remain to be higher than those for the kA. The «cost-effectiveness» analysis related to «the proportion of stable patients» for the horizons of 6 and 12 months indicated that the reproduced AA would be more economically effective than the kA. However, when the observation period was increased to 24 months, this economic advantage of AA diminished, and the kA drugs had a lower CeR instead. For the «number of hospitalization-free days per year», the use of AA was more cost-effective only versus the 100% use of reproduced AA at the simulated horizon of 12 months. When the use of 100% reference AA or the combined use of reference + reproduced AA was simulated, the treatment with kA remained more economically effective, regardless of the simulated period.Conclusion. The pharmacoeconomic simulation of the antipsychotic therapy in outpatients with schizophrenia suggests the ways to optimize the treatment. Among them, (a) using AA for the treatment of at least 15.6% of patients (those who are employed); keeping the ≥60% use of kA to ensure the optimal resource-saving effect of the treatment; (b) using reproduced AA at the level of ≥70% (instead of the reference AA similar in efficacy and safety) to keep the treatment economically feasible.
Analisis model pengelolaan zakat dan wakaf (Studi Yayasan Yatim Mandiri Cabang Malang)
Authors: Solekah Nihayatu Aslamatis 
Published: August 2018

Among the objectives of waqaf in islam is the distribution of welfare among the people and poverty eradication. When well managed endowments will support the development both in economic, religious, social, cultural, political, defense and scurity. The purpose of this study was to examine the management model of zakat and waqf productive. The result of this study are expected to provide benefits to provide a more substantial to the scientists, researchers, readers and the public that there is a model of productive management of waqf grant that can be used as an example in the management of zakat and waqf productive. The result showed that the foundation is independent orphans have an independent organizational system, in which the form of institutions such as the institute amil zakat generally resulting in organizational system there is no interfence from goverment regulations. Constraints/ problems experienced in Yayasan Yatim Mandiri Malang from the internal aspect is the lack qualified Human Resources an Human Resource Capibility less creative. Constraints/ problems faced in the Orphan Foundation of Malang independent external aspect is still modest community thought lack of support from family. To resolve the internal problems of orphans independent foundation that is to prepare a Qualified Human Resource (Internal). As for the external problem than it should maximize the marketing and provide understanding to families or orphans
PENGARUH PEMBIAYAAN MURABAHAH DAN MUSYARAKAH MELALUI KREDIT BERMASALAH TERHADAP LIKUIDITAS BANK UMUM SYARIAH
Authors: Laksmi Puteri Sayyida Islamiya , Solekah Nihayatu Aslamatis 
Published: August 2018

One of the causes of bankruptcy is not due to losess incurred but due to the inability to maintain cashflow availability. This study aims to determine the direct relationship of murabahah and musyarakah financing to Islamic bank liquidity through the NPF ratio. This study is descriptive quantitative study using path analysis and purposive sampling to determine the sample. The results of this study indicate that murabahah financing has no effect on NPF but effect the liquidity of Islamic bank. On the contrary, for musyarakah financing affecting NPF but not for liquidity of Islamic bank. It can be implied that due to differences in risk coverage of the two contract (murabahah financing dan musyarakah financing) where murabahah financing the level of risk that may arise will be more borne by the recipient of the financing. This contrasts with the musyarakah financing agreement where the risk Non performing loan can be minimized by sharing the risk in musyarakah financing.However, its relationship with bank liquidity, in fact murabahah financing provides a large contribution considering that islamic bank get certainty about the profit ratio of this financing agreement. As for musyarakah financing, Islamic bank liquidity actually decreases due to uncertainty in the profit sharing ratio it receives. From the result of the intervening test, NPF proxied by the ratio of NPF of Islamic banks did not mediate murabahah financing and musyarakah financing to Islamic bank liquidity.
The Interaction Effect of the Correlation between Dimensions and Item Discrimination on Parameter Estimation
Authors: Göçer Şahin Sakine , Çakıcı Eser Derya , Gelbal Selahattin 
Published: August 2018

There are
some studies in the literature that have considered the impact of modeling
multidimensional mixed structured tests as unidimensional. These studies have demonstrated
that the error associated with the discrimination parameters increases as the
correlation between dimensions increases. In this study, the interaction between
items’ angles on coordinate system and the correlations between dimensions was
investigated when estimating multidimensional tests as unidimensional. Data were
simulated based on two dimensional, and two-parameter compensatory MIRT model.
Angles of items were determined as 0.15o; 0.30o; 0.45o; 0.60o and
0.75orespectively. The correlations between
ability parameters were set to 0.15, 0.30, 0.45, 0.60 and 0.75 respectively,
which are same with the angles of discrimination parameters. The ability
distributions were generated from standard normal, positively and negatively
skewed distributions. A total of 75 (5 x
5 x 3) conditions were studied: five different conditions for the correlation
between dimensions; five different angles of items and three different ability
distributions. For all conditions, the number of items was fixed at 25 and the
sample size was fixed at n = 2,000.
Item and ability parameter estimation were conducted using BILOG. For each
condition, 100 replications were performed. The RMSE statistic was used to
evaluate parameter estimation errors, when multidimensional response data were scaled
using a unidimensional IRT model. Based on the findings, it can be concluded
that the pattern of RMSE values especially for discrimination parameters are
different from the existing studies in the literature in which multidimensional
tests were estimated as unidimensional.
Correlation of temperatures of melting and boiling of substances from the hypotheses of unpacking the substance
Authors: Haydarov Gennadiy Gasimovich , Haydarov Andrey Gennadyevich 
Published: August 2018

According to the properties of melting points and boiling points of the elements of the periodic table of D.I. Mendeleyev the application of the unpacking hypothesis of the substance was verified. The article contains the results of the verification. In general, according to the data for 98 substances taken from the information in Internet, convergence of results with an error of up to 40% for 73 substances was obtained. The effect of periodicity on the result is found. An exception to the calculated dependences was obtained for ten substances: noble gas and most halogens. A large discrepancy between the theoretical formulas and the reference data was also caused by the 14 elements of the middle groups.