bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRPV1 Promotes Periodontitis Tissue Inflammation and Oxidative Damage by Regulating STAT3 Signaling Pathway
Authors: Yu Mingzhu , Tian Huan , Lu Ruqing , Quan Ni , Qian Ling 
Published: December 2024

ABSTRACTAimsPeriodontitis is a chronic disease affecting adult oral health. Transient receptor potential vanilloid 1 (TRPV1) expression is shown to upregulate in many inflammatory diseases. Nevertheless, its biological potential along with the molecular mechanism in periodontitis is unclear. Our study aimed to explore the biological role and underlying signaling pathway of TRPV1 in periodontitis.MethodsIn the current research, human periodontal ligament stem cells (hPDLSCs) were stimulated by lipopolysaccharide (LPS) to induce inflammatory conditions in vitro. In vivo, the periodontitis mouse model was built by ligating the gingival sulcus of male C57BL/6J mice. Thereafter, the proliferation, apoptosis, inflammation, and oxidative stress‐related processes were assessed.ResultsWe found that LPS induced apoptosis and inflammation in hPDLCs, along with oxidative stress, while simultaneously inhibiting hPDLC proliferation (p < 0.05). Notably, TRPV1 expression was elevated in LPS‐treated hPDLSCs and gingival samples from patients with periodontitis. Interestingly, the increase in TRPV1 expression induced by Capsaicin, a TRPV1 agonist, inhibited cell proliferation while promoting LPS‐stimulated apoptosis, inflammation, and oxidative stress in hPDLSCs (p < 0.01). In contrast, inhibition of TRPV1 expression using Capsazepine, a TRPV1 inhibitor, produced opposite effects (p < 0.01). In vivo experiments revealed that inhibition of TRPV1 attenuated ligation‐induced periodontitis in mice, as evidenced by enhanced oxidative stress, inflammatory response, and elevated apoptosis (p < 0.01). Additionally, rescue assays indicated that TRPV1 promoted periodontitis‐associated tissue inflammation and oxidative damage via activating the STAT3 signaling pathway (p < 0.01).ConclusionOur study demonstrates that TRPV1 expression is high in periodontitis and facilitates periodontitis‐associated tissue inflammation and oxidative damage by regulating STAT3 signaling pathway, which implies that TRPV1 may represent a new therapeutic target for periodontitis.
Extreme altitude‐induced central sleep apneas lasting more than 100 seconds in a healthy 23‐year‐old man
Authors: Heiniger Grégory , Peci Arton , Marchi Nicola Andrea , Solelhac Geoffroy , Imler Théo , Waeber Adrien , Bradley Brian , Lecciso Gianpaolo , Wellman Andrew , Lovis Alban , Monney Pierre , Auberson Denise , Heinzer Raphael 
Published: December 2024

SummaryCentral sleep apneas (CSA) can occur de novo at high‐altitude in individuals without sleep‐disordered breathing at low altitude. These apneas are usually brief, lasting only 5–15 s. This report presents the first documented case of a man experiencing extreme altitude‐induced CSA lasting more than 100 s in the absence of any sleep breathing disorder in normoxia. A 23‐year‐old male with no pre‐existing health conditions was recruited for a study examining the work of breathing during sleep at a simulated altitude of 3500 m (FiO2:13%). A lowland polysomnography was first conducted to exclude moderate to severe sleep‐disordered breathing and showed an apnea–hypopnea index (AHI) of 7.6/h, an oxygen desaturation index (ODI) of 4.8/h, and a mean pulse oximetry‐based oxygen saturation (SpO2) of 93.9%. During the recording in the hypoxic chamber, the participant experienced prolonged CSA lasting up to 1 min and 49 s. These apneas were associated with significant oxygen desaturations (nadir: 44%). To investigate the origin of these atypical CSA, the participant underwent a new low‐altitude polysomnography with transcutaneous CO2 measurement (mean PaCO2:46 mmHg) and diurnal arterial blood gas analysis (pH: 7.42, pCO2: 35.1 mmHg, pO2: 79.9 mmHg, HCO3−: 22.4 mmol/L). These results indicated no signs of chronic hypercapnia or hypocapnia. A hypoxia tolerance test (FiO2: 11.5%) demonstrated a good ventilatory response to hypoxia during exercise (1.004 L/min/kg). A rebreathing test according to the Read protocol in hyperoxia demonstrated an impaired ventilatory response to CO2 (<0.6 L/min/mmHg). This report documents a rare form of extreme hypoxia‐induced CSA, potentially caused by impaired CO2 chemoreceptor sensitivity and an increased arousal threshold.
SCAR markers linked to Phytophthora capsici and Meloidogyne incognita resistance in Piper sp.
Authors: Truong Hai Thi Hong , Ho Nhi Thi Hoang , Rasphone Sonexay , Ho Han Ngoc 
Published: December 2024

AbstractBlack pepper (Piper nigrum) cultivation suffers from pathogenic Phytophthora capsici and Meloidogyne incognita, causing tremendous yield loss. To solve this problem, genetically sustainable disease‐resistant accessions need to be selected and bred. However, the lack of genomic DNA data for Piper sp. could be a major obstacle. In this study, we created R‐pools and S‐pools from resistant and susceptible accessions in bulked segregant analysis (BSA), respectively, selected DNA fragments associated with resistance or susceptibility to P. capsici and M. incognita from RAPD markers and then converted them into SCAR markers. This is the first publication showing the potential of molecular markers, specifically the SCAR marker, to select Piper sp. accessions resistant to both P. capsici and M. incognita. Based on BSA, four RAPD primers were selected: UBC#377, UBC#359, UBC#360 and UBC#408. Five resistance and susceptibility‐specific fragments were isolated, cloned and sequenced to synthesize 16 SCAR primers. We converted the UBC#377 marker into a 46‐377F2R1 marker and the UBC#360 marker into a 30‐360F1R2 marker. While the 46‐377F2R1 marker has the potential to identify P. capsici‐resistant accessions with a 350 bp resistance‐associated band, the 30‐360F1R2 marker showed the potential to find M. incognita‐resistant accessions with a 230 bp resistance‐linked band.
Characterisation of the Novel HLA‐C*07:971 Allele by Next‐Generation Sequencing
Authors: Gatouillat Grégory , Tabary Thierry , Tonye Libyh Marcelle , Pham Bach Nga , Giusti Delphine 
Published: December 2024

ABSTRACTHLA‐C*07:971 differs from HLA‐C*07:02:01:03 by a single nucleotide substitution in codon −4 in exon 1.
A Novel HLA‐C Allele, HLA‐C*12:419, Was Identified by Next‐Generation Sequencing in a Chinese Individual
Authors: Huang Ti‐Long , Zhang Tian‐Yao , Xiang Lin , Jiang Hong‐Chao , Zhang Xian‐Wen 
Published: December 2024

ABSTRACTHLA‐C*12:419 has a single nucleotide substitution at position 445 G>T when compared to the C*12:02:02:25 allele.
Characterisation of Novel HLA‐DQA1 and ‐DQB1 Alleles in a Spanish Bone Marrow Donor: DQA1*05:05:23 and DQB1*03:562
Authors: Ocejo‐Vinyals J. Gonzalo , Toriello María , Mota‐Pérez Nerea , García Mónica Renuncio , Balas Antonio 
Published: December 2024

ABSTRACTDescription of the novel HLA‐DQA1*05:05:23 and ‐DQB1*03:562 alleles.
Serological investigations on penicillin‐induced antibodies in the Thai population
Authors: Khumsuk Piyathida , Nathalang Oytip , Choychimplee Tanaporn , Sasikarn Wiradee , Intharanut Kamphon 
Published: December 2024

AbstractObjectivesThe study aimed to assess the conditions for coating RBCs with penicillin and examine the anti‐penicillin reactions of random Thai patients' sera against penicillin‐coated RBCs and normal sera from Thai donors testing in the presence of the drug.BackgroundPenicillin‐induced immunologic haemolytic anaemia (IHA) is reportedly related to possessing antipenicillin antibodies, immunoglobulin G (IgG), which has been identified in testing penicillin‐coated red blood cells (RBCs). In addition, low titre penicillin antibodies, often IgM, are detected in donors by testing in the presence of a solution of the penicillin.Materials and MethodsPenicillin‐coated RBCs were produced, and antipenicillin was tested against those penicillin‐coated RBCs amongst random Thai patients who had strongly positive direct antiglobulin (≥3+). Additionally, sera from Thai blood donors were tested in the presence of the penicillin. These relationships were determined by comparing the numbers of penicillin‐antibody positive patients with their diagnosis, sex, age and blood type.ResultsPenicillin requires a high pH to optimally adhere to RBCs that showed validated reactions with controls. Enrolment of 304 random patients, of whom 17 (5.59%) had positive antipenicillin tests using penicillin‐coated RBCs. Of the 246 donor samples, 3 (1.22%) displayed positive reactivities in the presence of soluble penicillin. Furthermore, no association was discovered between the patient's characteristics and antipenicillin positivity.ConclusionsThis is the first study to develop and report on the low percentage of patients' and donors' sera without IHA. Investigating suspected cases of penicillin‐induced IHA requires following our suggested method to identify clinically significant antipenicillin.
Cognitive Models for Machine Theory of Mind
Authors: Lebiere Christian , Pirolli Peter , Johnson Matthew , Martin Michael , Morrison Donald 
Published: December 2024

AbstractSome of the required characteristics for a true machine theory of mind (MToM) include the ability to (1) reproduce the full diversity of human thought and behavior, (2) develop a personalized model of an individual with very limited data, and (3) provide an explanation for behavioral predictions grounded in the cognitive processes of the individual. We propose that a certain class of cognitive models provide an approach that is well suited to meeting those requirements. Being grounded in a mechanistic framework like a cognitive architecture such as ACT‐R naturally fulfills the third requirement by mapping behavior to cognitive mechanisms. Exploiting a modeling paradigm such as instance‐based learning accounts for the first requirement by reflecting variations in individual experience into a diversity of behavior. Mechanisms such as knowledge tracing and model tracing allow a specific run of the cognitive model to be aligned with a given individual behavior trace, fulfilling the second requirement. We illustrate these principles with a cognitive model of decision‐making in a search and rescue task in the Minecraft simulation environment. We demonstrate that cognitive models personalized to individual human players can provide the MToM capability to optimize artificial intelligence agents by diagnosing the underlying causes of observed human behavior, projecting the future effects of potential interventions, and managing the adaptive process of shaping human behavior. Examples of the inputs provided by such analytic cognitive agents include predictions of cognitive load, probability of error, estimates of player self‐efficacy, and trust calibration. Finally, we discuss implications for future research and applications to collective human–machine intelligence.
Characterization of radiations‐induced genomic structural variations in Arabidopsis thaliana
Authors: Sall Salimata Ousmane , Alioua Abdelmalek , Staerck Sébastien , Graindorge Stéfanie , Pellicioli Michel , Schuler Jacky , Galindo Catherine , Raffy Quentin , Rousseau Marc , Molinier Jean 
Published: December 2024

SUMMARYDNA, is assaulted by endogenous and exogenous agents that lead to the formation of damage. In order to maintain genome integrity DNA repair pathways must be efficiently activated to prevent mutations and deleterious chromosomal rearrangements. Conversely, genome rearrangement is also necessary to allow genetic diversity and evolution. The antagonist interaction between maintenance of genome integrity and rearrangements determines genome shape and organization. Therefore, it is of great interest to understand how the whole linear genome structure behaves upon formation and repair of DNA damage. For this, we used long reads sequencing technology to identify and to characterize genomic structural variations (SV) of wild‐type Arabidopsis thaliana somatic cells exposed either to UV‐B, to UV‐C or to protons irradiations. We found that genomic regions located in heterochromatin are more prone to form SVs than those located in euchromatin, highlighting that genome stability differs along the chromosome. This holds true in Arabidopsis plants deficient for the expression of master regulators of the DNA damage response (DDR), ATM (Ataxia‐telangiectasia‐mutated) and ATR (Ataxia‐telangiectasia‐mutated and Rad3‐related), suggesting that independent and alternative surveillance processes exist to maintain integrity in genic regions. Finally, the analysis of the radiations‐induced deleted regions allowed determining that exposure to UV‐B, UV‐C and protons induced the microhomology‐mediated end joining mechanism (MMEJ) and that both ATM and ATR repress this repair pathway.
CORROSION BEHAVIOR OF REINFORCING STEEL IN RC SLAB CONTAINING CHLORIDES WITH PARTIAL REPLACEMENT OF CEMENT AS FLY ASH
Authors: Gupta Sristi Das , Sugiyama Takafumi , Islam Md Shafiqul 
Published: December 2024

Steel reinforcement in concrete containing fly ash has been practically employed to RC structures in snowy cold region and coastal areas so that the durability of the structures against corrosion can be enhanced. In this research to make the compatibility with RC slab bridge sodium chloride solution of 10[WU1]Â % in concentration was applied on RC slab and corrosion development was monitored by electrochemical method. Applying fly ash in RC slab at two replacement levels of 15[WU2]Â % (F15) and 30% (F30) of cement the specimens were observed. The observation result verified that fly ash concrete showed longer period of corrosion initiation (ASTM C876) than normal reinforced concrete. The initiation period of corrosion was 4.5 and 6 times longer for F15 and F30 concrete than normal concrete, as well as 91 days strength of F30 concrete was about 14% higher that of norma concrete. Test result showed that fly ash has better influence on steel corrosion reduction than concrete cover increment. It was observed that fly ash concrete (F15 and F30) with 3 cm concrete cover has better corrosion resistivity than using 4 cm cover of non-fly ash concrete. Furthermore, using the same concrete cover (3 cm) it was found that the actual corrosion rate was decreased about 68 to 82% by adding fly ash 15 to 30% respectively compared to normal reinforced concrete. In addition, a significant attenuation in corrosion area in rebar between fly ash concrete and normal concrete was found. Based on actual corrosion area on rebar surface, actual corrosion current density was larger than corrosion current density found from non-destructive way. Moreover, further analysis was conducted for characterization of different corrosion products using Raman spectroscopy with 532 nm wave length. It revealed that the corrosion product (Oxides and Oxyhydroxides compound) were less in F15 and F30 concrete compared to normal concrete. Considering these results, the possibility of reduction of chloride induced corrosion in reinforced concrete structure using fly ash has confirmed.Â
12-month outcomes after voretigene neparvovec gene therapy in paediatric patients with
RPE65
-mediated inherited retinal dystrophy
Authors: Daruich Alejandra , Rateaux Maxence , Batté Emilie , de Vergnes Nathalie , Valleix Sophie , Robert Matthieu P. , Bremond Gignac Dominique 
Published: December 2024

Aims
To report main outcomes and complications following voretigene neparvovec (Luxturna) treatment in paediatric patients.

Methods

Records of patients under the age of 17 treated by subretinal administration of voretigene neparvovec for confirmed biallelic
RPE65
-mediated inherited retinal dystrophy were retrospectively reviewed. Best-corrected visual acuity (BCVA) and data from spectral-domain optical coherence tomography, ultra-wide-field fundus imaging and Goldmann visual field (VF) were analysed at 12 months follow-up.

Results
12 eyes of six patients (mean age: 7.8 years) were analysed. No intraoperative complications occurred. BCVA significantly improved at 12-month follow-up (mean LogMAR (logarithm of the minimal angle of resolution) BCVA: 1.0±0.8 at baseline vs 0.6±0.3 at 12 months, p=0.001). Mean central macular thickness and central outer nuclear layer thickness did not change at 12 months follow-up. VF V4e isopter did not show significant changes. Postoperatively complications included: elevated intraocular pressure in two eyes of the same patient, a parafoveal lamellar hole at 3 months post-treatment and atrophy on the injection site observed in all eyes except one, which significantly enlarged during 12 months (p=0.008).

Conclusions
Most paediatric patients treated by voretigene neparvovec showed a significant increase in visual function at 12 months follow-up. None of the postoperative complications prevented gains in visual function.
Effects of concurrent aerobic and strength training in patients with type 2 diabetes: Bayesian pairwise and dose-response meta-analysis
Authors: Xue Han , Zou Yuehui , Zhang Shijie 
Published: December 2024

This study aimed to investigate the effects of concurrent aerobic and strength training (CT) in patients with type 2 diabetes and determine the most effective dose of CT. From the inception of the databases to March 2024, we conducted a systematic search of four electronic databases (PubMed, Embase, Web of Science, and Cochrane Library) to identify randomized controlled trials (RCTs) on CT intervention in patients with type 2 diabetes. Two independent authors assessed the risk of bias of the study using the Cochrane Risk of Bias Assessment Tools. Results analyzed included glycosylated hemoglobin (HbA1c), fasting blood glucose (FBG), body mass index, body fat percentage, blood pressure, and VO2max. Pairwise and dose-response meta-analyses using Bayesian hierarchical random-effects modeling were performed to analyze the effects of CT in patients with type 2 diabetes. From the inception of the databases to March 2024, we conducted a systematic search of four electronic databases (PubMed, Embase, Web of Science, and Cochrane Library) to identify randomized controlled trials (RCTs) on CT intervention in patients with type 2 diabetes. Two independent authors assessed the risk of bias of the study using the Cochrane Risk of Bias Assessment Tools. Results analyzed included glycosylated hemoglobin (HbA1c), fasting blood glucose (FBG), body mass index, body fat percentage, blood pressure, and VO2max. Pairwise and dose-response meta-analyses using Bayesian hierarchical random-effects modeling were performed to analyze the effects of CT in patients with type 2 diabetes. A total of 1948 participants (935 males) were included in 23 RCTs. The male/female ratio of participants was 52/48; the mean age range was 50–65 years. The results show that CT significantly reduced HbA1c levels (MD=−0.48%, 95% CrI: −0.55 to −0.40), with some heterogeneity among different levels (SD=0.31, 95% CrI: 0.17 to 0.51), and the model converged well. Similarly, FBG levels were also significantly improved (MD=−0.48 mmol/L, 95% CrI: −0.55 to −0.40), with greater heterogeneity (SD=17.73, 95% CrI: 11.23 to 28.09). Additionally, we found a non-linear dose-response relationship between CT and HbA1c levels, with an optimal dose of 1030 METs-min/week (MD=−0.47%, 95% CrI: –0.68 to –0.26, SE=0.11). CT significantly improves several health indicators in patients with type 2 diabetes. A non-linear dose-response relationship was observed between the training dose of CT and HbA1c, and it is recommended that 270 min of moderate-intensity CT or 160 min of vigorous-intensity CT be performed weekly.
PROSPERO registration number: CRD42024547119.

Keywords:meta-analysis; concurrent aerobic and strength training.
Maternal mortality following caesarean section in a low-resource setting: a National Malawian Surveillance Study
Authors: Riches Jennifer , Chimwaza Yamikani , Magreta Chakhame Bertha Immaculate , Milln Jack , Twabi Hussein H , Bilesi Rosemary , Gadama Luis , Kachale Fannie , Kuyere Annie , Makhaza Lumbani , Makuluni Regina , Munthali Laura , Musopole Owen , Ndamala Chifundo , Phiri Deborah A , Afran Louise , Wilson Amie , Thangaratinam Shakila , Merriel Abi , Waitt Catriona , Odland Maria Lisa , Jafali James , Lissauer David 
Published: December 2024

Background
Caesarean section (CS) is the most common major surgery conducted globally, with rates rising. CS also contributes to maternal morbidity and mortality, with increased risks in low-resource settings. We conducted a detailed review of maternal deaths from 2020 to 2022 in Malawi to determine the burden of deaths related to CS, avoidable health system factors, and causes of death associated with this procedure.

Methods
Data were collected regarding every maternal death occurring across all district and central hospitals in Malawi, alongside facility-level aggregated birth data. Maternal deaths were reviewed by facility-based multidisciplinary teams with subsequent confirmation of cause of death by obstetricians according to international criteria. Logistic regression was applied to estimate the odds of associations of leading causes of death with CS while adjusting for potential confounders.

Results
Despite a low national CS rate, most deaths occurred following CS (51.8%, 276/533). Women who delivered by CS were five times (OR 5.60, 95% CI 4.74 to 6.67) more likely to die than women who delivered vaginally. The leading causes of death following CS were postpartum haemorrhage (26.0%, 68/277), eclampsia (15.6%, 41/277) and infection (14.1%, 37/277). Deaths from pregnancy-related infection were more often associated with CS (OR 2.03, 95% CI 1.12 to 3.72). Health system factors more frequently associated with deaths following CS than vaginal birth included ‘prolonged abnormal observations without action’ (p=0.006), ‘delay in starting treatment’ (p=0.006) and ‘lack of blood transfusion’ (p=0.03).

Conclusions
We found a high burden of maternal death following CS in this low-resource setting. Until now, international attention and many clinical trials have been focused on improving the safety of vaginal birth. Our findings highlight the need to ensure the safe and appropriate use of this potentially life-saving intervention to reduce maternal deaths. To avoid the high burden of death following CS we highlight, there is urgent need to develop and trial CS-specific interventions.
Benign Transformation of Atypical Meningioma: A Rare Histopathological Phenomenon at Recurrence
Authors: Alanazi Rahaf F. , Alharbi Nasser , Alkhaibary Ali , AlSufiani Fahd , Aloraidi Ahmed 
Published: December 2024

Background and importance: Meningiomas are one of the most frequent primary central nervous system (CNS) tumors. According to the World Health Organization (WHO) classification of brain tumors, meningiomas are categorized into Grade 1 (benign meningioma; 80%), Grade 2 (atypical meningioma; 4%–15%), and Grade 3 (anaplastic meningiomas; 1%–3%). Grade 2 meningioma has a higher recurrence rate, ranging from 29%–52%. However, the transformation from atypical meningioma into benign meningioma is poorly understood. The present article describes a patient with Grade 2 meningioma that transformed into a benign subtype.Case presentation: A 51‐year‐old female with a history of seizures, presented with left‐sided progressive weakness. Radiological imaging revealed a large extra‐axial parasagittal lesion measuring 5 cm × 5 cm × 4.8 cm, suggestive of meningioma. The patient underwent subtotal resection of the lesion. Histologically, the tumor was in favor of Grade 2 meningioma. Radiological follow‐up 8 years postoperatively revealed a recurrent meningioma. The patient underwent right‐sided craniotomy and resection of the parasagittal meningioma. The histopathological features were suggestive of a Grade 1 meningioma.Conclusion: The transformation from atypical to benign meningiomas is rarely reported and the mechanism remains unclear. The present case provides insights into the natural history of this entity, describes possible etiologies, and lists the surgical management with an emphasis on preoperative radiological imaging and histopathological investigations.