bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zum Entwerfen im Schalenbau: der neue Bistro‐Pavillon „Subway“ in Kelley Barracks, Stuttgart
Authors: Wilhelm Viktor , Brixner Robert 
Published: April 2019

AbstractDie Verwendung von modularen Holzschaltischen ermöglicht die Wiederbelebung des Betonschalenbaus in Form von Faltwerken. Während einer „Stagnationsphase“ im Schalenbau haben sich jedoch die normativen bauphysikalischen und bautechnischen Anforderungen an die Betonbauten verändert. Am Beispiel der Dachschale des neuen „Subways“ innerhalb der „Kelley Barracks“ Stuttgart‐Möhringen werden im nachfolgenden Bericht die wesentlichen Aspekte des Entwerfens und Realisierens moderner Faltwerke angesprochen. In Zusammenhang mit der höheren Fertigungsfreundlichkeit ihrer Formen konnte unter Verwendung eines Faltwerks ein wirtschaftliches Ergebnis erzielt werden. Ihr Vorteil liegt des Weiteren in der einfachen Durchbildung von Temperaturstößen bzw. Temperaturblöcken. Nicht zuletzt haben die Autoren beim Entwurf der Dachschale die Einbaubarkeit von linearen thermischen Isokörben mitentwickelt.
Comparison of expression optimization of new derivative of staphylokinase (SAK‐2RGD‐TTI) with the rSAK
Authors: Faraji Habibollah , Ramezani Mohammad , Mashkani Baratali , Sadeghnia Hamid R. , Benhangi Hamid M. , Hosseini Teshnizi Saeed , Soltani Fatemeh 
Published: April 2019

AbstractStaphylokinase (SAK) is a promising thrombolytic agent for the treatment of patients suffering from blood‐clotting disorders. To increase the potency of SAK and to minimize vessel reocclusion, a new construct bearing SAK motif fused to tsetse thrombin inhibitor (TTI) via a 20‐amino acid linker with 2 RGD (2 × arginine‐glycine‐aspartic acid inhibiting platelet aggregation via attachment to integrin receptors of platelet) was codon optimized and expressed comparatively in Pichia pastoris GS115 as a Mut+ strain and KM71H as a Muts strain. Fusion protein was optimized in terms of best expression condition and fibrinolytic activity and compared with the rSAK. Expression level of the designed construct reached up to 175 mg/L of the culture medium after 72‐hr stimulation with 2.5% methanol and remained steady for 3–4 days. The highest expression was obtained at the range of 2–3% methanol. The SAK‐2RGD‐TT (relative activity >82%) was more active at 25–37 °C than rSAK (relative activity of 93%). Further, it showed relative activity >80% at pH ranges of 7–9. Western blot analysis showed two bands of nearly 27 and 24 kDa at ratio of 5 to 3, respectively. The specific fibrinolytic activity of the SAK‐2RGD‐TTI was measured as 8,269 U/mg, and 19,616 U/mg for the nonpurified and purified proteins, respectively. Deglycosylation by using tunicamycin in culture medium resulted in higher fibrinolytic activity of SAK‐2RGD‐TTI (2.2 fold). Consequently, compared to the rSAK, at the same equimolar proportion, addition of RGD and TTI fragments could increase fibrinolytic activity. Also, P. pastoris can be considered as an efficient host for overexpression of the soluble SAK‐2RGD‐TTI with high activity without requiring a complicated purification procedure.
Recent Chemical Approaches for Site‐Specific Conjugation of Native Antibodies: Technologies toward Next‐Generation Antibody–Drug Conjugates
Authors: Yamada Kei , Ito Yuji 
Published: April 2019

AbstractAntibody–drug conjugates (ADCs), which consist of three components, antibody, linker, and payload, can function as “magic bullets”. These conjugates offer the ability to target drug delivery to specific cells, based on cell‐specific recognition and the binding of an antigen by a monoclonal antibody (mAb). In particular, by delivering a cytotoxic payload to cancer cells, ADCs are expected to provide a breakthrough in oncology treatments by providing a way to increase efficacy and decrease toxicity, in comparison with traditional chemotherapeutic treatments. The development of ADC therapeutics has dramatically progressed in the past decade and two ADCs have been approved and used as anticancer drugs in the clinic. However, several critical issues regarding the performance of ADCs are still being discussed and investigated. Indeed, in the past few years, several groups have reported that, changing the number and position of the drug payloads in the ADCs, affects the pharmacokinetics, drug release rates, and biological activity. The use of conventional heterogeneous conjugation methods for ADC preparation results in the drug/antibody ratio and connecting position of the payload having stochastic distributions. Therefore, it is important to investigate how these potential problems can be circumvented through site‐specific conjugation. Herein, various site‐specific chemical conjugation strategies with native mAbs that are currently used for the production of ADCs, including residue‐selective labeling for generating ADCs, disulfide rebridging, and affinity‐peptide‐mediated site‐specific chemical conjugation technologies, are reviewed and described.
Chrysophanol attenuated isoproterenol‐induced cardiac hypertrophy by inhibiting Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway
Authors: Yuan Jing , Hong Huiqi , Zhang Yuhong , Lu Jing , Yu Youhui , Bi Xueying , Wang Junjian , Ye Jiantao 
Published: April 2019

AbstractCardiac hypertrophy is a common pathological change found in various cardiovascular diseases. Although it has long been recognized as an important risk factor responsible for heart failure, there is still a lack of effective treatments in clinic. Chrysophanol is a natural compound with multiple biological activities and protective roles in the cardiovascular system. However, its potential effect on cardiac hypertrophy remains unclear. In the current study, we found that chrysophanol could protect against isoproterenol (ISO)‐induced cardiac hypertrophy both in vitro and in vivo. Increase of cell surface and hypertrophic marker expression induced by ISO in neonatal rat cardiomyocytes was downregulated by chrysophanol. Moreover, chrysophanol ameliorated the abnormal changes of cardiac structure and function in rats subjected to ISO injection, as shown by echocardiography and morphometry measurements. Further mechanistical investigation demonstrated that chrysophanol inhibited phosphorylation of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3) induced by ISO. Nuclear translocation of STAT3 and transcription of downstream genes promoted by ISO treatment were also remarkably suppressed by chrysophanol. Taken together, our findings revealed that chrysophanol attenuated ISO‐induced cardiac hypertrophy by inhibiting JAK2/STAT3 signaling pathway. Chrysophanol may be a potential candidate compound for the prevention and treatment of hypertrophy‐related cardiomyopathy.
Neurodegeneration, demyelination, and astrogliosis in rat spinal cord by chronic lead treatment
Authors: Villa‐Cedillo Sheila Adela , Nava‐Hernández Martha Patricia , Soto‐Domínguez Adolfo , Hernández‐Ibarra José Anselmo , Perez‐Trujillo José Juan , Saucedo‐Cárdenas Odila 
Published: April 2019

AbstractEarly exposure to lead (Pb) has been associated with an elevated risk of developing neurodegenerative diseases. There is evidence that neuronal damage in chronic Pb exposure can be caused by the convergence of glial damage. Apoptosis may be a possible mechanism of Pb‐induced cell death in the central nervous system. We tested cellular damage and apoptosis in the spinal cord of Wistar rats treated with Pb. Twelve rats were divided into two groups (n = 6): the control group was treated with only drinking water and the other group received 500 ppm of Pb acetate. After 3 months of Pb treatment, all animals were euthanized and spinal cords were extracted. Morphology was evaluated by Nissl and Kluver‐Barrera stainings. Apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Specific antibodies were used to evaluate Pb damage in oligodendrocytes, astrocytes, and microglia. A large number of apoptotic bodies was observed in the white matter of the Pb‐treated group. The Pb‐treated group also showed a reduced number of neurons and oligodendrocytes but had an increased number of astrocytes compared with the nontreated group. Our results demonstrate that chronic Pb treatment induces neurodegeneration, demyelination, and astrogliosis in the rat spinal cord.
Velocity and attenuation analysis methods for characterizing the properties of wheat flour noodle dough
Authors: Strybulevych Anatoliy , Diep Sally , Daugelaite Daiva , Guillermic Reine‐Marie , Page John H. , Hatcher David W. , Scanlon Martin G. 
Published: April 2019

AbstractBackground and objectivesNoodles are a traditional staple of the Asian diet, and ensuring quality during production is important for both producers and consumers. Ultrasound is sensitive to the mechanical properties of dough that relate to product texture and is therefore a promising quality assessment tool. For optimal results, it is important to assess the merits of different ultrasonic analysis methods.FindingsMechanical properties of raw Asian noodle dough made from two red spring wheat varieties were measured by a low‐frequency ultrasonic transmission technique. Entire transmitted ultrasonic pulses, having passed through disks of the noodle dough, were analyzed using fast Fourier transform (FFT) techniques, enabling the transmitted phase and amplitude as a function of frequency to be compared for samples of different thickness. This approach allows noodles made from different wheat varieties to be discriminated through ultrasonic velocity and attenuation measurements.ConclusionsThe frequency dependence of both velocity and attenuation coefficient, which was revealed the FFT method, points to the presence of gas bubbles, which even in small amounts can influence texture and hence noodle quality.Significance and noveltyIn addition to providing insights on properties that influence noodle quality, the frequency dependence of velocity and attenuation found by the FFT analysis can explain differences reported by different analysis techniques.
Black Current: Structure, Characterization, and Optoelectronic Properties of Ce3Cl3[MoO6]
Authors: Dorn Katharina V. , Blaschkowski Björn , Bamberger Heiko , van Slageren Joris , Doll Klaus , Claus Ralf , Matsumoto Yosuke , Takagi Hidenori , Hartenbach Ingo 
Published: April 2019

AbstractThe admixture of CeO2, Ce, CeCl3, and MoO3 with an excess of LiCl as flux in evacuated silica ampules leads to large black single crystals as well as a black microcrystalline powder of Ce3Cl3[MoO6] after tempering at 850 °C for three days. The title compound crystallizes in the hexagonal space group P63/m (a=934.93(4), c=538.86(2) pm) with two formula units per unit cell. The crystal structure consists of rather unusual trigonal‐prismatic [MoO6]6− units besides Ce3+ ions in a tetra‐capped trigonal‐prismatic coordination, formed by four Cl− and six O2− ions. The black color is related to an optical band gap of 1.35(2) eV, which was determined by diffuse reflectance spectroscopy and confirmed by theoretical calculations. The low band gap between the 4f1 state of cerium (HOMO) and the 5d0 state of molybdenum (LUMO) gave rise to the idea of electronic excitation between these two states by IR irradiation, creating a drop in the resistivity of the material, which was detected by appropriate measurements.
One‐Pot Sequential Kumada–Tamao–Corriu Couplings of (Hetero)Aryl Polyhalides in the Presence of Grignard‐Sensitive Functional Groups Using Pd‐PEPPSI‐IPentCl
Authors: Sinha Narayan , Champagne Pier Alexandre , Rodriguez Michael J. , Lu Yu , Kopach Michael E. , Mitchell David , Organ Michael G. 
Published: April 2019

AbstractWe report a general and rapid chemoselective Kumada–Tamao–Corriu (KTC) cross‐coupling of aryl bromides in the presence of chlorides or triflates with functionalized Grignard reagents at 0 °C in 15 min by using Pd‐PEPPSI‐IPentCl (C4). Nucleophiles and electrophiles (or both) can contain Grignard‐sensitive functional groups (‐CN, ‐COOR, etc.). Control experiments together with DFT calculations suggest that transmetallation is rate limiting for the selective cross‐coupling of Br in the presence of Cl/OTf with functionalized Grignard reagents. One‐pot sequential KTC/KTC cross‐couplings with bromo–chloro arenes have been demonstrated for the first time. We also report the one‐pot sequential KTC/Negishi cross‐couplings using C4 showcasing the versatility of this methodology.
Synthesis of novel chiral metal complexes derived from chiral thiosemicarbazide ligands as potential antioxidant agents
Authors: Başaran Eyüp , Sıcak Yusuf , Sogukomerogullari Hatice Gamze , Karaküçük‐Iyidoğan Ayşegül , Oruç‐Emre Emine Elçin , Sönmez Mehmet , Öztürk Mehmet 
Published: April 2019

AbstractTwo new chiral thiosemicarbazide ligands and their Cu (II), Ni (II), Pd (II), and Zn (II) complexes were synthesized and characterized by nuclear magnetic resonance (NMR) (only for ligand), Fourier transform infrared (FT‐IR), ultraviolet visible (UV‐Vis), mass, and elemental analysis. The antioxidant activity of ligands and their metal complexes was examined. It was found that the antioxidant activity of metal complexes was better than their ligands. In addition, the antioxidant activity, as reflected by free radical scavenging, was evaluated. Besides, Pd (II) complexes exhibited better antioxidant activity than Ni (II), Cu (II), and Zn (II) complexes. Therefore, complexes (3a‐Pd and 3b‐Pd) can be used as an antioxidant agent or antioxidant test standard.
Resolution of (R,S)‐ibuprofen catalyzed by immobilized Novozym40086 in organic phase
Authors: Yuan Xin , Wang Lujun , Liu Guangyong , Dai Guilin , Tang Kewen 
Published: April 2019

AbstractThe enantioselective esterification of ibuprofen catalyzed by Novozym40086 was successfully conducted in organic solvent. Removing‐water reagent was added into the reaction mixture to remove water produced in the esterification. The effects of temperature, n‐hexanol concentration, ibuprofen concentration, and loading of enzymes were investigated. Under the condition of equilibrium, the thermodynamic equilibrium constant (K) of 7.697 and enantioselectivity (E) of 8.512 were obtained. The esterification reaction achieved its equilibrium in approximately 30 hours with conversion of 56% and eeS of 93.78%. The predicted values of X and eeS were 67.90% and 95.60%, respectively. The experimental value is approximately equal to the theoretical value, which indicates the feasibility of ideal models.
Catalyst‐Free Hydroxytrifluoromethylation of Alkenes UsingIodotrifluoromethane
Authors: Su Zhaoben , Guo Yong , Chen Qing‐Yun , Zhao Zhi‐Gang , Nian Bao‐Yi 
Published: April 2019

Summary of main observation and conclusionThe importance of CF3‐containing molecules in pharmaceuticals, agrochemicals and materials intrigues the intense interest in synthetic methodology of these compounds. With a purpose to enrich trifluoromethylation methodology, we carefully examined the substrate scope of hydroxytrifluoromethylation of alkenes using iodotrifluoromethane, and the reaction provided β‐trifluoromethyl alcohols in good yields under extremely mild conditions without catalysts. We found that our reaction can be applied to not only styrenes but also various aliphatic alkenes with excellent selectivity; no ketone was detected in most of our cases. Another feature of our discovery is “simple”. The reaction was carried out in air, irradiated by visible light, at room temperature and most importantly no catalyst was needed. A solution of CF3I in DMSO was used as the facile trifluoromethylating reagent, which simplified the utilization of gaseous CF3I. Based on 19F NMR spectroscopy, we observed a halogen bond between CF3I and tertiary amine in this reaction. The interaction may promote single electron transfer by the visible light irradiation.
Medicare and patient spending among beneficiaries diagnosed with chronic myelogenous leukemia
Authors: Kenzik Kelly M. , Bhatia Ravi , Williams Grant R. , Bhatia Smita 
Published: April 2019

BackgroundThe authors examined Medicare spending and patient spending in older patients with chronic myelogenous leukemia (CML) over the first 5 years from the time of CML diagnosis in the tyrosine kinase inhibitor (TKI) era.MethodsMedicare beneficiaries with CML who were diagnosed between 2007 and 2012 at age >65 years were identified from the Surveillance, Epidemiology, and End Results (SEER)–Medicare database (805 beneficiaries). A noncancer Medicare beneficiary sample was frequency‐matched based on age, sex, and race/ethnicity (805 individuals). Patients were followed until 5 years from diagnosis, disenrollment, death, or December 31, 2014, whichever came first. Total Medicare spending, service‐specific spending, and amount owed by patients was estimated monthly and then summed over 60 months and averaged to generate annual spending.ResultsThe median age at the time of diagnosis of CML was 76 years (range, 66‐102 years). Overall, 51.4% of patients received TKIs (27.8% received imatinib alone), 28% received non‐TKI therapy, and 21% received no treatment. The 5‐year survival rate for patients with ≥85% time receiving TKIs was 79% compared with 76% for noncancer controls versus 62% for those with <85% time receiving TKIs. Annual Medicare spending was found to be significantly higher for patients treated with TKIs ($143,053) compared with those treated without TKIs ($41,268 vs $10,498 for noncancer controls). Annual patient cost responsibility was $11,712 per patient receiving any TKIs versus $7330 for those receiving non‐TKI outpatient chemotherapy versus $3561 for noncancer controls.ConclusionsOlder patients with CML with adequate time receiving TKI therapy have 5‐year survival rates that are comparable to those of their counterparts without cancer. However, TKI use is accompanied with significant Medicare and patient spending; patients receiving multiple TKIs (ie, dasatinib or nilotinib along with imatinib) constitute the group with the highest spending.
Accelerating magnetic induction tomography‐based imaging through heterogeneous parallel computing
Authors: Walker David W. , Kramer Stephan C. , Biebl Fabian R. A. , Ledger Paul D. , Brown Malcolm 
Published: April 2019

SummaryMagnetic Induction Tomography (MIT) is a non‐invasive imaging technique, which has applications in both industrial and clinical settings. In essence, it is capable of reconstructing the electromagnetic parameters of an object from measurements made on its surface. With the exploitation of parallelism, it is possible to achieve high quality inexpensive MIT images for biomedical applications on clinically relevant time scales. In this paper we investigate the performance of different parallel implementations of the forward eddy current problem, which is the main computational component of the inverse problem through which measured voltages are converted into images. We show that a heterogeneous parallel method that exploits multiple CPUs and GPUs can provide a high level of parallel scaling, leading to considerably improved runtimes. We also show how multiple GPUs can be used in conjunction with deal.II, a widely‐used open source finite element library.
Temperature‐Dependent Photoluminescence of Silver‐Indium‐Sulfide Nanocrystals in Aqueous Colloidal Solutions
Authors: Stroyuk Oleksandr , Raevskaya Alexandra , Spranger Felix , Gaponik Nikolai , Zahn Dietrich R. T. 
Published: April 2019

AbstractThe temperature dependence of the photoluminescence (PL) intensity of colloidal semiconductor nanocrystals (NCs) makes them an appealing option in bio‐sensing applications. Here, we probed the temperature‐dependent PL behavior of aqueous glutathione (GSH)‐capped Ag−In−S (AIS) NCs and their core/shell AIS/ZnS heterostructures. We show that both core and core‐shell materials reveal strong PL quenching upon heating from 10 to 80 °C, which is completely reversible upon cooling. The PL quenching is assigned to the thermally activated dissociation of complexes formed by ligands with the metal cations on the NC surface and the introduction of water into the NC coordination sphere. This unique mechanism of the thermal PL quenching results in a much higher temperature sensitivity of the aqueous colloidal AIS (AIS/ZnS) NCs as compared with previously reported analogs capped by covalently bound ligands. Our results are expected to stimulate further studies on aqueous ternary NCs as colloidal luminescent nano‐thermometers applicable for ratiometric temperature sensing.
On the improvement of positioning accuracy in WiFi‐based wireless network using correntropy‐based kernel learning algorithms
Authors: Xue Nan , Luo Xiong , Wu Jinsong , Wang Weiping , Wang Long 
Published: April 2019

AbstractCurrently, we have witnessed the rapid development of data‐driven machine learning methods, which have achieved very effective results in communication systems. Kernel learning is a typical nonlinear learning method in the machine learning community. This article proposes two novel correntropy‐based kernel learning algorithms to improve the accuracy of indoor positioning in WiFi‐based wireless networks. In general, correntropy as a measure of local similarity defined in kernel space can be used for robust signal processing to address large outliers. Then, through the combination of the maximum mixture correntropy criterion (MMCC) and online vector quantization (VQ), we develop a learning algorithm, named quantized kernel MMCC (QKMMCC) method, which works with the advantage of correntropy while effectively suppressing the growth of memory structure and reducing the computation in this algorithm using VQ. Furthermore, to fully use redundant information and to further improve the learning accuracy, an intensified QKMMCC, called QKMMCC_BG, is also proposed on the basis of the bilateral gradient (BG) technique. Simulation results show that, compared with some similar approaches, our algorithms can achieve better computational performance. In addition, our proposed algorithms are also applied to indoor positioning of WiFi‐based wireless networks. The experimental results show that our kernel learning algorithms can effectively improve the positioning accuracy. The average positioning errors of our two algorithms in the experiment are 0.86 m and 0.76 m, respectively. The effectiveness of our algorithms is further verified.