bluuebunny/crossref_metadata_2025_split huggingface dataset:
In silicoAnalysis of L-Glutaminase from Extremophiles
Authors: Devi Sarita , Savitri  , Raj Tilak , Sharma Nikhil , Azmi Wamik 
Published: September 2018

Background:L-glutaminase enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. Protein L-glutaminase, which converts amino acid glutamine to a glutamate residue, is useful as antileukemic agent, antiretroviral agent and a new food-processing enzyme.Objective:The sequences representing L-glutaminase from extremophiles were analyzed for different physico-chemical properties and to relate these observed differences to their extremophilic properties, phylogenetic tree construction and the evolutionary relationship among them.Methods:In this work, in silico analysis of amino acid sequences of extremophilic (thermophile, halophile and psychrophiles) proteins has been done. The physiochemical properties of these four groups of proteins for L-glutaminase also differ in number of amino acids, aliphatic index and grand average of hydropathicity (GRAVY).Result:The GRAVY was found to be significantly high in thermophilic (2.29 fold) and psychrophilic bacteria (3.3 fold) as compare to mesophilic bacteria. The amino acid Cys (C) was found to be statistically significant in mesophilic bacteria (approximately or more than 3 fold) as compared to the abundance of this amino acid in extremophilic bacteria.Conclusion:Multiple sequence alignment revealed the domain/motif for glutaminase that consists of Ser-74, Lys-77, Asn-126, Lys-268, and Ser-269, which is highly conserved in all microorganisms.
Association of PPARG Gene Polymorphisms Pro12Ala with Type 2 Diabetes Mellitus: A Meta-analysis
Authors: Li Junyan , Niu Xiaohong , Li JianBo , Wang Qingzhong 
Published: September 2018

Background:Previous studies suggested that the single nucleotide polymorphisms of Pro12Ala located within the PPARG gene were significantly associated with the T2DM. Recently, the genetic studies on Pro12Ala were conducted in the different ethnic groups and the results of each study were shown to be inconsistent. Moreover, the systematic review has not been updated since 2000.Objective:To further validate the risk of Pro12Ala for T2DM disease based on the genetic data.Methods:The genetic studies on the Pro12Ala in the T2DM were searched in the PubMed and PMC database from January 2000 to October 2017. The meta-analysis was conducted with the CMA software.Results:The meta-analysis collected 14 studies including 20702 cases and 36227 controls. The combined analysis of all studies found that Pro12Ala was shown to be significantly associated with T2DM and the Ala allele played the increasing risks for the disease. Nevertheless, publication bias was detected in the combined analysis. The subgroup analysis indicated that Pro12Ala was found to be significant in the Caucasian and Chinese population. There was no heterogeneity and publication bias in these two groups.Conclusion:The meta-analysis confirmed the evidence that the Pro12Ala was the susceptible variant for the decreasing risks for the T2DM
Pinocembrin-Enriched Fractions of Elytranthe parasitica (L.) Danser Modulates Apoptotic and MAPK Cellular Signaling in HepG2 Cells
Authors: Kumar Nimmy , Shrungeswara Akhila H. , Mallik Sanchari B. , Biswas Subhankar , Mathew Jesil , Nandakumar Krishnadas , Mathew Jessy , Lobo Richard 
Published: September 2018

Background:
Hepatocellular carcinoma (HCC) is the fifth leading cause of cáncer mortality. Elytranthe
parasitica (L.) Danser (EP), a hemiparasitic plant (Loranthaceae) has potent anti-cancer properties.

Objective:
In the study, we investigated the effect of EP fractions on the expression of apoptosis and mitogenactivated
protein kinase (MAPK) markers deregulated in HCC. Bioactivity fractionation was performed to isolate the
phytochemical(s) exerting anti-tumor activity in HepG2 cells.

Method:
Anti-proliferative, clonogenic and anti-metastatic effects of EP fractions were examined in hepatocellular
carcinoma cell line, HepG2 by Sulphorhodamine B, colony formation and scratch wound assays respectively in
hepatocellular cell line, HepG2. The effects of EP fractions on key markers of apoptosis and MAPK signaling pathways
were explored.
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Key findings: EP bioactive fractions showed significant anti-tumor potential, reduced clonogenicity and considerably
inhibited cell migration in HepG2 cells in vitro. The fractions augmented annexin V binding and induced apoptosis
by causing cell cycle arrest at G2/M and S phase checkpoints. The fractions increased expression levels of p53, bad,
cleaved PARP (Poly ADP ribose polymerase) and cleaved Caspase-3. Expression levels of phosphorylated ERK1/2
(Extracellular signal-regulated kinase) were downregulated. Pinocembrin-7-O-ß-D-glucoside and chrysin were isolated
and characterized for the first time from Elytranthe parasitica (L.) Danser.

Conclusion:
Our findings reveal that EP fractions induced cell cycle arrest and triggered apoptosis in HepG2 cells by
upregulating apoptosis and deactivating MAPK pathway. It signifies that pinocembrin glycoside and chrysin are
bioactive phytochemicals contributing to the potent anti-hepatocarcinoma effects on HepG2 cells. Hence, bioactive
EP fractions could be used as a therapeutic agent for effective HCC therapy.
Pengobatan Malaria Kombinasi Artemisinin (ACT) di Provinsi Papua Barat Tahun 2013
Authors: Kinansi Revi Rosavika , Mayasari Rika , Pratamawati Diana Andriyani 
Published: September 2018

Malaria is still a disease with highest incidence rate in Indonesias. Based on Riskesdas 2013, the prevalence of malaria in West Papua was generally increasing. This study aimed to find the correlation of type of malaria found in blood examination and distribution status of ACT. Samples of data obtained by stratified random sampling from 1490 people who had suffered from malaria in West Papua. Data analysis using univariate descriptive and correlation analysis The result showed that the most common type of malaria was tertiana malaria caused by Plasmodium vivax (51%). Early detection performed within the first 24 hours when the patient is suffering from fever can be used as the basis for a policy that early detection can reduce malaria morbidity. It can be concluded ACT suitable for any type of malaria. The concistency of provision of ACT can be implemented by increasing public awareness of taking prophylactic. In addition the ideal combination antimalarial drugs be able to heal in a short time and if the patients performs the compliance of taking the drug, it will not be antimalarial resistance.
Daya Saing Kawin Nyamuk Jantan Steril (Culex quinquefasciatus) Skala Laboratorium: Studi Awal Penggunaan Teknik Serangga Mandul dalam Pengendalian Vektor Filariasis Limfatik di Kota Pekalongan
Authors: Ramadhani Tri , Hadi Upik Kusuma , Soviana Susi , Irawati Zubaidah , Sunaryo Sunaryo 
Published: September 2018

Culex quinquefasciatus is the main vector of limfatic filariasis in Pekalongan City. Sterile Insect Tehnique could be an alternative vector control efforts to eliminate filariasis. The success of this technique is depend on the ability of laboratory-reared sterile males with the wild-type females. Indicator of SIT Aplication is determined by the value of the mating competitiveness and sterility to Culex quinquefasciatus (Diptera:Culicidae). The design of the research is an experimental. Gamma irradiation on the pupae (age . 15 hours) with the doses of 0 Gy, 60 Gy, 65 Gy,70 Gy, 75 Gy and 80 Gy in BATAN Jakarta. Male mosquitoes which emerged from the pupa then matting with a normal female. This research observed the mean of females laying eggs ,fecundity, fertility and mating competitiveness. This experimental research was conducted in the laboratory and the data were analyzed by ANOVA.The result showed that irradiation at the trial doses had an effect on fertility of Culex quinquefasciatus, but not had significant effect on fecundity and mating competitiveness . A dose of 70 Gy is the optimum dose with a fertility rate of 1.8% (sterility 98.2%) and C indexs 0,568 can be recommended for futher semi field assays. The number of sterile males were six times compared with the wild population to increase the chances of mating with wild-type females.
Parameter Entomologi pada Daerah Endemis Demam Berdarah Dengue Tinggi dan Rendah di Jawa Tengah (Studi di Kabupaten Kudus dan Wonosobo)
Authors: Ikawati Bina , Wahyudi Bondan Fajar , Astuti Novia Tri , Sunaryo Sunaryo 
Published: September 2018

Kudus district is a district with high DHF cases in Central Java in 2013 (IR=57.50). Wonosobo District is new area with low DHF cases (IR=11.92). Aedes aegypti is the main vector of DHF in both. The purpose of this study was to determine the difference of entomological parameter in high and low DHF endemic area. This research was continued analysis of entomologycal data from study of The Resistance Map of Aedes aegypti (Linn) Agains Cypermethrin 0,05%, Malathion 0,8% and Temephos inn Purworejo, Kebumen, Pekalongan, Demak, Wonosobo, Cilacap, Kudus, Klaten, Banjarnegara at 2014, that use cross sectional design. The results showed that HI, CI, BI and PI in Kudus (40,67%,21,40%, 233,67 and 113,67%), higher than in Wonosobo (14,33%, 10,93%, 15,33 dan 38,33%). In all location bathing tub were the potential breeding places of Ae. aegypti. In Kudus, Breeding Preference Ratio (BPR) in barrel and water tank for “wudhu” had the highest as much as 4.03. In Wonosobo the highest BPR was in jar as much as 9.15. Multivariat analysis showed that in Kudus container covers were most closely correlated to the presence of Ae. aegypti larvae, while in Wonosobo illumination around containers is the main factor.
Re-Transmission Assessment Survey Filariasis Pasca Pengobatan Massal di Kabupaten Agam, Provinsi Sumatera Barat Tahun 2016
Authors: Santoso Santoso , Cahyaningrum Sri 
Published: September 2018

Agam District finished the repetition of Filariasis treatment for 2 years, so Agam must be conducting the Re-TAS. Re-TAS was conducted on elementary school students grade 1 and 2 in the district of Agam. Survey design using the school cluster. The number of schools selected were 40 schools. Selection of the sample schools were calculated using sample survey builder (SSB). All students grades 1 and 2 in selected schools were examined using Brugia Rapid test to assess the presence of specific antibodies against Brugia malayi and B. timori worms. The number of students who registered as many as 1,999 children, while being examined by RDT as many as 1,717. A total of 282 students were absent during the examination or refused to be examined.Test results showed that 3 positive, 1694 negative, 20 invalid and 3 samples were excluded. The number of samples taken into account to assess the transmission of filariasis as many as 1,697, which is only positive and negative samples. The survey found that the minimum sample was adequate and the number of positive children under the cut off point, so Agam passed TAS 1.
Research Concerning the Influence of Soil Tillage Systems on Weeds Control and Corn Hybrids Yield Cultivated in Southeastern Romania
Authors: Chițoi Claudiu , Ciontu Costică , Toader Maria 
Published: September 2018

Abstract
The paper presents the yield results obtained in 2017 for the corn crop grown in the soil and climate conditions of Southern Romania (Calarasi county). The purpose of the research was to analyze the behavior and yield of a range of corn hybrids from different FAO groups: DKC 4590 (FAO 350), PP9911 (FAO 410) and Olt (FAO 430) under different soil tillage systems. The conventional tillage system applied was plowing at 20 and 30 cm, while scarifying at 30 and 40 cm and Tiger 3 MT at 20 and 30 cm were used for the minimum tillage system. The average yield of the three hybrids analyzed was 8,130.3 kg/ha in the conventional tillage system (plowing at 20 and 30 cm) with mechanical weeding, while for the conventional tillage system without mechanical weeding the yield was 7,682.2 kg/ha. The minimum tillage variants (scarifying and Tiger 3 MT) recorded an average yield of 6,746.4 kg/ha when mechanical weeding was applied and 6,474.9 kg/ha for the variants without mechanical weeding. Regarding the degree of weeding by tillage system, in the conventional tillage system (plowing at 20 and 30 cm) with mechanical weeding the number of weeds was 4.2 plants/m2 and 9.3 plants/m2 for the variants without mechanical weeding. The minimum tillage variants (scarifying and Tiger 3 MT) recorded 12.8 weeds/m2 when mechanical weeding was applied and 16.2 weeds/m2 for the variants without mechanical weeding.
Researches Concerning Sunflower Seeds Treatment against Tanymecus Dilaticollis, in Green House Conditions
Authors: Georgescu Emil , Toader Maria , Ionescu Alina Maria , Cană Lidia , Râşnoveanu Luxiţa 
Published: September 2018

Abstract
In the last years sunflower become one of the most important crops in Romania. The most of the surface cultivated with sunflower from our country is located in south and south-east and, in many cases, sunflower is cultivated after maize. As result the attack of the maize leaf weevil (Tanymecus dilaticollis Gyll) at sunflower plants has increasing. The attack is dangerous when sunflower plants are in first vegetation stages (BBCH 10-BBCH 14). Similar with maize crop, sunflower seeds treatment with neonicotinoids were generalized after 2007. As result of the EU directive 485/2013, no active ingredients remain available for sunflower seeds treatment, against T. dilaticollis, in Romania. The researches from the last years were orientated for finding of the possible alternatives for seed treatments, in eventuality of permanent ban of neonicotinoid insecticides, after 2018. Because of the variability of climatic conditions from the field, during spring period, researches concerning effectiveness testing of sunflower seed treatments, in green house conditions, using high pest pressure is a good method to evaluate influence of the different active ingredients to control T. dilaticollis adults. In this experiment it has tested three doses of cyantraniliprole active ingredient (7.5, 10.0 and 12.5 l/to) and three neonicothinoid active ingredients (imidacloprid, clothianidin, thiamethoxam). Sunflower seeds were placed in plastic pots. After plants emergence the insects were added in pots. At each pot it has added 20 adult insects, collected from the field. Each day, it has recorded insect mortality. At 8 days from the start of the experiment, it has assessed attack intensity of the adult insects on sunflower plants. The attack was evaluated using a scale from 1 (plant not attacked) to 9 (plant complete destroyed). In conditions of high pest pressure (4 insects/plant) the attack intensity of T. dilaticollis at sunflower plants was ranged from 3.35 in case of variant treated with clothianidin and 8.50 in case of untreated plants. Lower attack intensity of the pest at sunflower plants it has registered in case of variants treated with imidacloprid and thiamethoxam (I=3.58 and 3.50). Plants emerged from the untreated seeds were destroyed by the insects, at 8 days from the start of the experiment.
Effects of Combined Application of Bradyrhizobium Japonicum and Pseudomonas Putida on Nutrients and Water Contents of Soybean in Relation to Soil Moisture Regime
Authors: Rotaru Vladimir 
Published: September 2018

Abstract
Plant growth promoting rhizobacteria (PGPR) are of growing interest for their potential role in improving soil fertility and enhancing crops productivity and nutrients contents. In order to assess the impact of PGPR on nutrients contents of soybean, a greenhouse experiment was carried out with Bradyrhizobium japonicum applied alone or in combination with Pseudomonas putida in conjunction with fertilization of P or manure. Two soil moisture regimes were installed: 70% of whole holding capacity (WHC) as control and 35% WHC as moderate water stress. The water deficit was imposed at the flowering stage for 12 days. Plants cultivated under P insufficiency and drought exhibited lower physiological parameters in treatment with single inoculation of B. japonicum than combined application of two rhizobacteria. The results showed that the combined application of rhizobacteria enhanced the N and P contents of soybean regarding of soil water conditions. The integrated use of rhizobacteria improved the relative water content of soybean. Their influence was more pronounced under P deficiency and manure fertilization of plants especially under no stress water conditions. Hence, combined application of B. japonicum and P. putida has a potential to improve nutrition and growth of soybean under normal soil moisture as well as under moderate drought.
Influence of Different Storage Methods on Apples Chemical Proprieties
Authors: Sumedrea Dorin , Florea Alina , Sumedrea Mihaela , Asănica Adrian , Coman Radu , Militaru Mădălina , Chiţu Emil , Butac Mădălina , Marin Florin Cristian , Calinescu Mirela 
Published: September 2018

Abstract
The objective of preservation is to keep fruit fresh as long as possible after harvesting, without major physical, chemical or biological changes in their composition. The experimental factors underlying it the organization scheme are: A Factor - apple varieties: ‘Idared’, ‘Goldrush’, ‘Florina’, ‘Pinova’, ‘Dalinette’, ‘Golden reinderes‘,‘Golden lassa‘,‘Ariane‘; B factor - storage methods, with three graduations: classical method - low temperature and high humidity (1-4ºC; humidity 85-90%), Janny MT box storage method (1-4 ºC; 95-100% humidity; O2 1-3%; CO2 2-5%), fruit control equipment box-pallets (1- 4ºC; 90-95% humidity; O2 1-3%; CO2 2-5%) and factor C - fruit storage period -at 3, 4 and 5 months after harvest respectively. On the average of the cultivars taken in the study, on observe the tendency to increase the total dry mater and total sugar content, and decrease the total tritrable acidity and vitamin C with the prolongation of the fruit storage period.
Enzyme Activity as an Indicator of Soil Quality in Sterile Waste Heap
Authors: Dodocioiu Ana Maria , Babeanu Cristina , Buzatu Diana Gilda 
Published: September 2018

Abstract
Recent research reports that enzymes accumulated in soil participate in the cycles of elements and contribute to soil fertility. The purpose of this paper is to determine the enzymatic activity and the humus content in the materials from waste heap in order to assess their fertility and to establish possible re-cultivation technologies. The investigations were carried out on materials from the Pesteana North waste heap. In order to achieve this, the activity of catalase, invertase, urease and phosphatase activity was determined in different plots of the heap during the summer and as a control the zonal soil was used. Enzymatic activities were colorimetric assay using appropriate substrates. The obtained results show a large variation in the values of the determined enzymatic activities. In all investigated plots, all enzymes are found to be lower than those determined for the zonal soil. A strong positive correlation is observed between enzymatic activities of catalase and invertase and the accumulation of humus which demonstrates that enzymatic activities has a useful tool for studying the evolution of heap materials to agricultural soils.
The Presence of Iron and Iucc Virulence-Associated Genes in Romanian Apec Isolates
Authors: Gurău Maria Rodica , Hameed Hasan Majid , Popp Mihaela Cristiana , Campeanu Marius Valer , Daneș Doina 
Published: September 2018

Abstract
Colibacillosis in poultry is relating with the colonisation with so called Avian Pathogen E. coli (APEC) strains. It is already known that usually in the APEC isolates are present at least 13 virulence-associated genes. We selected 12 non-repetitive E. coli isolates from different Romanian poultry outbreaks. Isolates have been evaluated for the presence of the virulence-associated genes, iroN and iucC. The DNA extraction was made using QIAamp cador Pathogen Mini Kit (Qiagen). The amplification protocol was: a cycle of denaturation at 94°C for 5 min followed by 35 cycles of 94°C for 30 s, 63°C for 45 s and 72°C for 105 s, and a cycle of 72°C for 7 min. Both virulence-associated genes were identified in 83.33% (11/12) isolates. In two APEC isolates, only one gene was identified, iroN or iucC, respectively. According to these preliminary results it could be assumed that iroN and iucC genes are independently expressing their virulence.
Fredholm type integral equation with special functions
Authors: Ayant Frdric , Kumar Dinesh 
Published: September 2018

Abstract
Recently Chaurasia and Gill [7], Chaurasia and Kumar [8] have solved the one-dimensional integral equation of Fredholm type involving the product of special functions. We solve an integral equation involving the product of a class of multivariable polynomials, the multivariable H-function defined by Srivastava and Panda [29, 30] and the multivariable I-function defined by Prasad [21] by the application of fractional calculus theory. The results obtained here are general in nature and capable of yielding a large number of results (known and new) scattered in the literature.
On generalized Laguerre matrix polynomials
Authors: Batahan Raed S. , Bathanya A. A. 
Published: September 2018

AbstractThe main object of the present paper is to introduce and study the generalized Laguerre matrix polynomials for a matrix that satisfies an appropriate spectral property. We prove that these matrix polynomials are characterized by the generalized hypergeometric matrix function. An explicit representation, integral expression and some recurrence relations in particular the three terms recurrence relation are obtained here. Moreover, these matrix polynomials appear as solution of a differential equation.
Fixed point iterations for Prešić-Kannan nonexpansive mappings in product convex metric spaces
Authors: Fukhar-ud-din Hafiz , Berinde Vasile 
Published: September 2018

AbstractWe introduce Prešić-Kannan nonexpansive mappings on the product spaces and show that they have a unique fixed point in uniformly convex metric spaces. Moreover, we approximate this fixed point by Mann iterations. Our results are new in the literature and are valid in Hilbert spaces, CAT(0) spaces and Banach spaces simultaneously.
Some properties of analytic functions related with Booth lemniscate
Authors: Najmadi P. , Najafzadeh Sh. , Ebadian A. 
Published: September 2018

Abstract
The object of the present paper is to study of two certain subclass of analytic functions related with Booth lemniscate which we denote by ℬ𝒮 (α) and ℬ𝒦 (α). Some properties of these subclasses areconsidered.
The Relationship between Current Account Deficits and Growth in Montenegro: ARDL Bounds Testing Approach
Authors: Özer Mustafa , Žugić Jovana , Tomaš-Miskin Sonja 
Published: September 2018

Abstract
In this study, we investigate the relationship between current account deficits and growth in Montenegro by applying the bounds testing (ARDL) approach to co-integration for the period from the third quarter of 2011 to the last quarter of 2016. The bounds tests suggest that the variables of interest are bound together in the long run when growth is the dependent variable. The results also confirm a bidirectional long run and short run causal relationship between current account deficits and growth.
The short run results mostly indicate a negative relationship between changes in the current account deficit GDP ratio and the GDP growth rate. This means that any increase of the value of independent variable (current account deficit GDP ratio) will result in decrease of the rate of GDP growth and vice versa.
The long-run effect of the current account deficit to GDP ratio on GDP growth is positive. The constant (β0) is positive but also the (β1), meaning that with the increase of CAD GDP ratio of 1 measuring unit, the GDP growth rate would grow by 0,5459. This positive and tight correlation could be explained by overlapping structure of the constituents of CAD and the drivers of GDP growth (such as tourism, energy sector, agriculture etc.).
The results offer new perspectives and insights for new policy aiming for sustainable economic growth of Montenegro.