bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinicopathologic features and outcomes of hepatic inflammatory pseudotumour (IPT) and hepatic IPT‐like lesions
Authors: Nguyen Eric D , Wen Kwun Wah , Kakar Sanjay , Balitzer Dana J 
Published: October 2024

AimsHepatic inflammatory pseudotumours (IPTs) are nonneoplastic hepatic masses characterized by variably fibroblastic stroma and inflammatory infiltrate, hypothesized to arise as part of a response to infection or prior surgery. The aim of this study was to evaluate the clinicopathologic features and outcomes of biopsy‐proven hepatic IPT as well as other cases with IPT‐like histologic features.Methods and ResultsA database search at our institution identified cases with a pathologic diagnosis of hepatic IPT (n = 80) between 2000 and 2023. Histologic features (stromal quality, inflammatory cell components, granulomas, and necrosis) were evaluated. Past medical and surgical history, microbiologic studies, and outcomes were reviewed retrospectively. Patients frequently had a past medical history of malignancy (34%), biliary disease (15%), or prior intraabdominal surgery (24%), and often presented with multifocal hepatic lesions (36%). Variable inflammatory backgrounds were present, including histiocytic (36%), lymphoplasmacytic (34%), or neutrophilic (24%). Specific organisms were identified in 15% of cases, most commonly Klebsiella and Staphylococcus species. Most patients with available clinical follow‐up demonstrated radiologic resolution and/or had repeat negative biopsy; a minority of patients (8%) were subsequently diagnosed with neoplastic hepatic lesions. No significant association was seen between histologic features and the subsequent clinical or pathologic diagnosis of hepatic neoplastic lesions.ConclusionsHepatic IPT is a heterogeneous entity that can present in a variety of clinical scenarios and show a wide morphologic spectrum. These lesions often regress spontaneously or with antibiotics. A subset of cases with hepatic IPT‐like histologic features were subsequently diagnosed with malignancy, emphasizing the need for continued follow‐up and repeat biopsy depending on clinical and radiologic features.
Consumer Perspectives on Food Traceability—A Systematic Literature Review and Future Research Agenda
Authors: Chaudhary Anam , Sharma Rajat , Vemireddy Vidya 
Published: October 2024

ABSTRACTFood traceability is a critical approach that ensures the safety and quality of food. It has garnered significant attention, particularly in the aftermath of multiple food safety incidents reported in various countries. While there is extant literature available on consumer perspectives on food traceability, the findings are not consistent across studies. This systematic literature review aims to provide a comprehensive understanding by integrating findings from past studies. It includes 61 articles sourced from two databases, namely Scopus and Web of Science. This review outlines various stages of a consumer's decision for food traceability, starting from exposure, awareness, understanding, liking and disliking, attitudes and preferences, purchase intentions and willingness to pay, to the final purchase. It also identifies the determinants of consumer responses to food traceability, categorising them as factors internal to the consumers, determinants due to the perception of external factors and contextual factors. Moreover, it identifies gaps in the existing research and proposes research questions to expand the knowledge in this area.
Digital Approach Provides Predictability in Increasing the VDO in Erosive Wear: Clinical Technique and 9‐Year Follow‐Up
Authors: Ibrahim Fady , Plaster Udo , Gierthmuehlen Petra C. 
Published: October 2024

ABSTRACTObjectiveThis clinical case describes a digital workflow using face scans and CAD/CAM technology for a full‐mouth rehabilitation with increased vertical dimension of occlusion (VDO) with adhesive lithium disilicate restorations after 9 years.Clinical ConsiderationsA healthy, 46‐year‐old man displaying severe tooth wear, underwent an extensive full‐mouth rehabilitation involving an increase of the VDO through laminate veneers and adhesive partial coverage lithium disilicate restorations. Anatomical landmarks of the face and reference planes were captured using a digital face scanning system. The anatomical position of the maxilla was registered with a transfer device. Digital data sets of the intraoral situation combined with a facial scan enabled precise virtual planning, guiding minimally invasive preparations. Long‐term provisional restorations, milled from high‐performance polycarbonate, were used to test the novel VDO before the final lithium disilicate restorations were fabricated. This approach provided a time‐ and cost‐efficient treatment solution. No failures were observed at the 9‐year follow‐up.ConclusionsUtilizing face scans and design software enable a virtual visualization and comprehensive quality control for patients with dental wear, resulting in significant time savings and increased predictability for the dental technician, dentist and patient.Clinical SignificanceThe described digital workflow optimizes the planning and implementation of a comprehensive adhesive full‐arch rehabilitation with an increase of the VDO. The clinical long‐term follow up result of CAD/CAM assisted minimally invasive lithium disilicate restorations after 9 years demonstrates to clinicians a reliable treatment concept for patients with dental wear.
Therapeutic targets for hepatocellular carcinoma identified using proteomics and Mendelian randomization
Authors: Zhu Weixiong , Fan Chuanlei , Liu Bo , Qin Jianqi , Fan Aodong , Yang Zengxi , Zhang Hui , Zhou Wence 
Published: October 2024

AbstractBackground and AimHepatocellular carcinoma (HCC) emerges as a formidable malignancy marked by elevated morbidity and mortality rates, coupled with a dismal prognosis. The revelation of gene–protein associations has presented an avenue for the exploration of novel therapeutic targets.MethodsPooling plasma proteomic data (seven published GWAS) and HCC data (DeCODE cohort), we applied MR to identify potential drug targets, which were further validated in the FinnGen cohort and UK Biobank. Subsequent colocalization and summary‐data‐based Mendelian randomization analyses were performed for potential associations of this set of proteins. In addition, enrichment information pathways were investigated in depth by KEGG pathway analysis, single‐cell sequencing, PPI and DGIdb, ChEMBL, and DrugBank database analyses, specific cell types enriched for expression were identified, interacting proteins were identified, and finally, druggability was assessed.ResultsIn summary, the levels of 10 proteins are linked to HCC risk. Elevated levels of TFPI2 as well as decreased levels of ALDH1A1, KRT18, ADAMTS13, TIMD4, SCLY, HRSP12, TNFAIP6, FTCD, and DDC are associated with increased HCC risk. Notably, HRSP12 show the strongest evidence. These genes are primarily expressed in specific cell types within the HCC TME. Moreover, intricate protein–protein interactions, involving key players like ALDH1A1 and RIDA, ALDH1A1 and DDC, and ALDH1A1 and KRT18, contribute significantly to the amino acid metabolism and dopaminergic neurogenesis pathway. Proteins such as ALDH1A1, KRT18, TFPI2, and DDC are promising targets for HCC therapy and broader cancer drug development. Targeting these proteins offers substantial potential in advancing HCC treatment strategies.ConclusionsThis research delineates 10 protein biomarkers linked to HCC risk and offers novel perspectives on its etiology, as well as promising avenues for the screening of HCC protein markers and therapeutic agents.
Feasibility of endoscopic resection for superficial laryngopharyngeal cancer after radiotherapy
Authors: Ashizawa Hiroshi , Yamamoto Yoichi , Mukaigawa Takashi , Kawata Noboru , Maeda Yuki , Yoshida Masao , Minamide Tatsunori , Hotta Kinichi , Imai Kenichiro , Ito Sayo , Takada Kazunori , Sato Junya , Ishiwatari Hirotoshi , Matsubayashi Hiroyuki , Ono Hiroyuki 
Published: October 2024

AbstractBackground and AimWe aimed to elucidate the feasibility of endoscopic resection (ER) for salvage and metachronous lesions following chemoradiotherapy (CRT) and radiotherapy (RT) for laryngopharyngeal cancer.MethodsConsecutive patients who underwent ER for superficial laryngopharyngeal cancer between March 2005 and September 2022 were retrospectively reviewed and stratified into salvage (16 patients, 16 lesions), metachronous (18 patients, 27 lesions), and naïve RT (217 patients, 306 lesions) groups. Salvage lesions were residual or local recurrent after CRT, and metachronous lesions were second primary lesions in the irradiated field following complete response. Short‐term outcomes were evaluated, including R0 resection, en bloc resection, procedure time, adverse events, local recurrence rate, and clinical course.ResultsThe en bloc resection rates were 88%, 93%, and 88%; R0 resection rates were 50%, 52%, and 56%; procedure times were 25, 27, and 25 min, in the salvage, metachronous, and naïve RT groups, none of which were significantly different among the three groups. Although adverse event rates tended to be higher in the salvage and metachronous groups than in the naïve RT group, the differences were not significant (18%, 15%, and 8%). The cumulative local recurrence rates at 2 years were 21%, 13%, and 6%, significantly higher in the salvage group than in the naïve RT group. The local control rates with ER alone were 94%, 93%, and 97%; none were significantly different among the three groups.ConclusionER for salvage and metachronous superficial lesions can be safely performed, suggesting its acceptability as a minimally invasive treatment.
Risk of colorectal cancer among fecal immunochemical test‐positive individuals by timing of previous colonoscopy: A multicenter analysis
Authors: Kawamura Takuji , Oda Yasushi , Toyoizumi Hirobumi , Kato Masayuki , Sekiguchi Masau , Takamaru Hiroyuki , Mizuguchi Yasuhiko , Horiguchi Go , Kobayashi Kiyonori , Sada Miwa , Yokoyama Akira , Utsumi Takahiro , Tsuji Yosuke , Ohki Daisuke , Takeuchi Yoji , Shichijo Satoki , Ikematsu Hiroaki , Matsuda Koji , Teramukai Satoshi , Kobayashi Nozomu , Matsuda Takahisa , Saito Yutaka , Tanaka Kiyohito 
Published: October 2024

AbstractBackground and AimThe risk of colorectal cancer among fecal immunochemistry test‐positive individuals who had undergone previous colonoscopies remains unclear. Therefore, this study aimed to determine the differences in the risk of colorectal cancer among fecal immunochemistry test‐positive individuals according to the timing of their previous colonoscopies.MethodsThis multicenter, retrospective, observational study was conducted in Japan as a subgroup analysis of the J‐SCOUT study (UMIN000040690), which integrated and analyzed a database comprising all colonoscopies performed at participating Japanese institutions between 2010 and 2020. This study used colonoscopy data of fecal immunochemistry test‐positive individuals aged ≥ 20 years from three facilities that entered the timing of previous colonoscopies into the endoscopy database. Histologically confirmed advanced neoplasia was the study's primary outcome. Multivariate logistic regression analysis was used to calculate the odds ratios for each variable.ResultsIn total, 11,143 fecal immunochemistry test‐positive patients underwent colonoscopy during the study period. Of these, 10,160 patients were included in the analysis after excluding those who met the exclusion criteria. The overall advanced neoplasia detection rate was 9.38% (953/10,160; 95% confidence interval: 8.82–9.96%). Compared with the first colonoscopy, the odds ratios for advanced neoplasia in individuals who underwent colonoscopies 1, 2, 3, 4, 5, > 5, and ≥ 10 years previously were 0.27, 0.15, 0.06, 0.10, 0.29, 0.31, and 0.31, respectively.ConclusionsThe detection rates of advanced neoplasia were low among the fecal immunochemistry test‐positive individuals who had undergone colonoscopy, particularly in the past 5 years.
Understanding the clinical management of co‐occurring sleep‐related bruxism and obstructive sleep apnea in adults: A narrative and critical review
Authors: Dal Fabbro Cibele , Bornhardt‐Suazo Thomas , Landry Schönbeck Anaïs , de Meyer Micheline , Lavigne Gilles J. 
Published: October 2024

AbstractSleep‐related bruxism (SRB) is a motor oral behavior characterized by tooth grinding and jaw clenching activity, reported by 8%–12% of the adult general population and 3% of older individuals. The frequency of one of its biomarkers, rhythmic masticatory muscle activity (RMMA), remains elevated across ages. Obstructive sleep apnea (OSA) is associated with the brief and repetitive pause of breathing (apnea) and with transient reduction in oxygen (hypoxia). OSA is observed at all ages and in about 50% of older individuals with a male preponderance. SRB clinical assessment is based on self‐reporting of tooth grinding sound, awareness of clenching, jaw pain or headache, and clinical observation of tooth damage. OSA clinical assessment is based on sleepiness and fatigue, snoring, sleep quality, and awareness of breathing cessation, plus clinical examination of anatomical factors (e.g., obesity, retrognathia, large tonsil, macroglossia), age, gender, and body mass. Although the literature does not support association or causality between these two conditions, the co‐occurrence is reported in about 30%–50% of adults. To confirm a diagnosis of co‐occurring SRB and OSA, home sleep testing (HST) may be indicated. A sleep test is performed using electromyography (EMG) of jaw muscle (masseter or temporalis) and cardio‐respiratory variables (e.g., air flow, respiratory effort, oxygen level, heart rate). The management of co‐occurring SRB and OSA for individuals with prosthodontic needs is challenging to prevent compromising the oro‐pharyngeal space and breathing efficiency. OSA treatment in the presence of SRB includes continuous positive airway pressure (CPAP) use alone or with an occlusal splint or mandibular advancement device (MAD). In addition, the following may be considered: supine sleep correction device, myofuncional therapy, medications, and surgeries. All have limitations and risks. Individual variability suggests that phenotyping is mandatory to select the most efficient and personalized treatment.
Molecular mechanisms of the obesity associated with Bardet‐Biedl syndrome: An update
Authors: Zhong Bang‐Hua , Nie Ning , Dong Ming 
Published: October 2024

SummaryObesity is a prominent feature of Bardet‐Biedl syndrome (BBS), which represents a major and growing public health problem. More than half of BBS patients carry mutations in one of eight genes that encode subunits of a protein complex known as the BBSome, which has emerged as a key regulator of energy and glucose homeostasis. However, the mechanisms underlying obesity in BBS are complex. Numerous studies have identified a high prevalence of insulin resistance and metabolic syndrome among individuals with BBS. However, the exact mechanisms are not fully understood. This review summarized evidence from experiments using mouse and cell models, focusing on the energy imbalance that leads to obesity in patients with BBS. The studies discussed in this review contribute to understanding the functional role of the BBSome in the obesity associated with BBS, laying the foundation for developing new preventive or therapeutic strategies for obese patients.
CRISPR/Cas9‐Mediated Genome Editing for Trait Improvement and Stress Tolerance in Leguminosae (Legume Family)
Authors: Singh Sadhana , Mishra Vigya , Riyazuddin Riyazuddin , Chugh Vishal , Upadhyay Santosh Kumar 
Published: October 2024

ABSTRACTLegumes represent a significant source of protein, roughage, minerals and carbohydrates in global diets. They are cultivated primarily for human consumption, forage and as a green manure. However, the growth and productivity of legumes are often hindered by a range of biological and environmental factors. The most prevalent diseases afflicting legumes include downy mildew, fusarium root rot, southern blight and common leaf spot. Additionally, environmental stresses such as salinity and drought represents significant challenges. Over time, the development of cultivars with enhanced tolerance has emerged as a sustainable approach to combat the impact of these stresses. Although environmentally friendly, traditional breeding methods entail lengthy screening and cross‐breeding protocols, which constrain their efficacy in addressing climate challenges and ensuring global food security. Furthermore, these approaches rely on the natural availability of genetic variation, which may not always be exist for specific traits, particularly in the context of rapidly changing environmental conditions. Additionally, the traditional breeding method is not sufficiently precise, which can result in the unintended introduction of undesirable traits. Furthermore, the genetic intricacy of legumes presents an additional challenge making it difficult to isolate and enhance desired traits. These constrain impede the capacity to expeditiously develop cultivars that can withstand emerging stresses, such as climate change. Genome editing has emerged as a powerful tool to overcome the limitations of traditional breeding methods. By enabling precise and targeted genetic manipulation, genome editing can enhance desired traits or introduce new ones, thereby facilitating the development of climate‐resilient agronomic varieties. In particular, clustered regularly interspaced short palindromic repeat/CRISPR associated protein (CRISPR/Cas9)‐mediated editing has demonstrated considerable potential in enhancing legume adaptability to diverse stress conditions and increasing crop yield. This study highlights the advancements in genome editing, with a specific focus on CRISPR/Cas9 technology, as a means to improve legume crops' resilience and productivity in response to environmental pressures.
Chitinase 3‐like 1 overexpression aggravates hypoxia–reoxygenation injury in IEC‐6 cells by inhibiting the PI3K/AKT signalling pathway
Authors: Mi Lei , Jin Jie , Zhang Yingying , Chen Ming , Cui JianLi , Chen Rui , Zheng Xiao , Jing Changqing 
Published: October 2024

AbstractIntestinal ischaemia–reperfusion (I/R) is a common clinical pathology with high incidence and mortality rates. However, the mechanisms underlying intestinal I/R injury remain unclear. In this study, we investigated the role and mechanism of chitinase 3‐like 1 (CHI3L1) during intestinal I/R injury. Therefore, we analysed the expression levels of CHI3L1 in the intestinal tissue of an intestinal I/R rat model and explored its effects and mechanism in a hypoxia–reoxygenation (H/R) IEC‐6 cell model. We found that intestinal I/R injury elevated CHI3L1 levels in the serum, ileum and duodenum, whereas H/R enhanced CHI3L1 expression in IEC‐6 cells. The H/R‐induced inhibition of proliferation and apoptosis was alleviated by CHI3L1 knockdown and aggravated by CHI3L1 overexpression. In addition, CHI3L1 knockdown alleviated, and CHI3L1 overexpression aggravated, the H/R‐induced inflammatory response and oxidative stress. Mechanistically, CHI3L1 overexpression weakened the activation of the phosphoinositide 3‐kinase (PI3K)/AKT pathway, suppressed the nuclear translocation of Nrf2, and promoted the nuclear translocation of nuclear factor κB (NF‐κB). Moreover, CHI3L1 knockdown had the opposite effect on the PI3K/AKT pathway, Nrf2, and NF‐κB. Moreover, the PI3K inhibitor LY294002 blocked the effect of CHI3L1 knockdown on the H/R‐induced inhibition of proliferation, apoptosis, inflammatory response and oxidative stress. In conclusion, CHI3L1 expression was induced during intestinal I/R and H/R injury in IEC‐6 cells, and CHI3L1 overexpression aggravated H/R injury in IEC‐6 cells by inhibiting the PI3K/AKT signalling pathway. Therefore, CHI3L1 may be an effective target for controlling intestinal I/R injury.
Proteome profiling of polyomavirus nuclear replication centers using iPOND
Authors: Erickson Kimberly D. , Langsfeld Erika S. , Holland Alexandra , Ebmeier Christopher C. , Garcea Robert L. 
Published: October 2024

ABSTRACT

Polyomaviruses (PyVs) cause diverse diseases in a variety of mammalian hosts. During the life cycle, PyVs recruit nuclear host factors to viral genomes to facilitate replication and transcription. While host factors involved in DNA replication, DNA damage sensing and repair, and cell cycle regulation have been observed to bind PyV DNA, the complete set of viral and host proteins comprising the PyV replisome remains incompletely characterized. Here, the iPOND-MS technique (Isolation of Proteins on Nascent DNA coupled with Mass Spectrometry) was used to identify the proteome bound to murine PyV (MuPyV) DNA immediately following synthesis and 2 hours post-synthesis. Several novel MuPyV DNA interactors were identified on newly synthesized viral DNA (vDNA), including MCM complex members, DNA primase, DNA polymerase alpha, DNA ligase, and replication factor C. Though displaying partial overlap, the host and viral proteins bound to MuPyV DNA 2 hours post-synthesis lacked many of the replication proteins found on newly synthesized vDNA. These data help distinguish between the host factors critical for MuPyV DNA replication and those involved in downstream processing.

IMPORTANCE

Polyomaviruses are the causative agents of serious diseases in humans, including progressive multifocal leukoencephalopathy (PML), BK virus nephropathy, and Merkel cell carcinoma. The exact mechanisms by which the virus replicates, and which host cell proteins are required, are incompletely characterized. Identifying the host proteins necessary for efficient viral replication in the cell may reveal targets for downstream targets that may suppress viral replication
in vivo
.