bluuebunny/crossref_metadata_2025_split huggingface dataset:
AMPA receptors and seizures mediate hippocampal radial glia‐like stem cell proliferation
Authors: Shtaya Anan , Sadek Ahmed‐Ramadan , Zaben Malik , Seifert Gerald , Pringle Ashley , Steinhäuser Christian , Gray William Peter 
Published: October 2018

AbstractNeurogenesis is sustained throughout life in the mammalian brain, supporting hippocampus‐dependent learning and memory. Its permanent alteration by status epilepticus (SE) is associated with learning and cognitive impairments. The mechanisms underlying the initiation of altered neurogenesis after SE are not understood. Glial fibrillary acidic protein‐positive radial glia (RG)‐like cells proliferate early after SE, but their proliferation dynamics and signaling are largely unclear. We have previously reported a polarized distribution of AMPA receptors (AMPARs) on RG‐like cells in vivo and postulated that these may signal their proliferation. Here, we examined the acute effects of kainate on hippocampal precursor cells in vitro and in kainate‐induced SE on proliferating and quiescent clones of 5‐bromo‐2‐deoxyuridine prelabeled hippocampal precursors in vivo. In vitro, we found that 5 μM kainate shortened the cell cycle time of RG‐like cells via AMPAR activation and accelerated cell cycle re‐entry of their progeny. It also shifted their fate choice expanding the population of RG‐like cells and reducing the population of downstream amplifying neural progenitors. Kainate enhanced the survival of all precursor cell subtypes. Pharmacologically, kainate's proliferative and survival effects were abolished by AMPAR blockade. Functional AMPAR expression was confirmed on RG‐like cells in vitro. In agreement with these observations, kainate/seizures enhanced the proliferation and expansion predominantly of constitutively cycling RG‐like cell clones in vivo. Our results identify AMPARs as key potential players in initiating the proliferation of dentate RG‐like cells and unravel a possible receptor target for modifying the radial glia‐like cell response to SE.
Enhancing the representation of functional connectivity networks by fusing multi‐view information for autism spectrum disorder diagnosis
Authors: Huang Huifang , Liu Xingdan , Jin Yan , Lee Seong‐Whan , Wee Chong‐Yaw , Shen Dinggang 
Published: October 2018

AbstractFunctional connectivity network provides novel insights on how distributed brain regions are functionally integrated, and its deviations from healthy brain have recently been employed to identify biomarkers for neuropsychiatric disorders. However, most of brain network analysis methods utilized features extracted only from one functional connectivity network for brain disease detection and cannot provide a comprehensive representation on the subtle disruptions of brain functional organization induced by neuropsychiatric disorders. Inspired by the principles of multi‐view learning which utilizes information from multiple views to enhance object representation, we propose a novel multiple network based framework to enhance the representation of functional connectivity networks by fusing the common and complementary information conveyed in multiple networks. Specifically, four functional connectivity networks corresponding to the four adjacent values of regularization parameter are generated via a sparse regression model with group constraint (
l2,1‐norm), to enhance the common intrinsic topological structure and limit the error rate caused by different views. To obtain a set of more meaningful and discriminative features, we propose using a modified version of weighted clustering coefficients to quantify the subtle differences of each group‐sparse network at local level. We then linearly fuse the selected features from each individual network via a multi‐kernel support vector machine for autism spectrum disorder (ASD) diagnosis. The proposed framework achieves an accuracy of 79.35%, outperforming all the compared single network methods for at least 7% improvement. Moreover, compared with other multiple network methods, our method also achieves the best performance, that is, with at least 11% improvement in accuracy.
Economic benefit of leptospirosis prevention in Kelantan, Malaysia: Willingness‐to‐contribute approach
Authors: Saizan Sarah , Sukeri Surianti , Zahiruddin Wan Mohd , Shafei Mohd Nazri , Rukman Awang Hamat , Malina Osman , Jamaluddin Tengku Zetty Maztura Tengku , Aziah Daud 
Published: October 2018

SummaryLeptospirosis is an endemic disease in Malaysia. Despite the increasing incidence rate, knowledge on the economic assessment of preventing leptospirosis is still limited. This paper introduces the willingness‐to‐contribute (WTC) method for estimating the economic benefit of preventing leptospirosis. A cross‐sectional study using the WTC method was applied to measure how much time respondents in Kelantan were willing to contribute toward preventing leptospirosis. Study respondents were wet market traders aged 18 years old and above who were fluent in the Malay language. The average WTC value was multiplied by the population of Kelantan to derive the monetary value of preventing leptospirosis. Two hundred and fifty respondents participated in the study. The mean time contribution was 6.68 hours (SD9.01) per month. The average WTC corresponded to a monthly cost savings of US$4.94 per person. Approximately between US$106.7 million to US$315 million per annum can be saved through the prevention of leptospirosis in Kelantan. Preventing leptospirosis is beneficial to Kelantan and would bring major economic savings. The findings are intended to help policy makers in the planning and management of leptospirosis policies and interventions.
Using a personalized clinical decision support system for bromdihydrochlorphenylbenzodiazepine dosing in patients with anxiety disorders based on the pharmacogenomic markers
Authors: Zastrozhin Michael S. , Sorokin Aleksandr S. , Agibalova Tatyana V. , Grishina Elena A. , Antonenko Anastasiya Р. , Rozochkin Ilya N. , Duzhev Denis V. , Skryabin Valentine Y. , Galaktionova Tatyana E. , Barna Ilya V. , Orlova Anna V. , Aguzarov Albert D. , Savchenko Ludmila M. , Bryun Evgeny A. , Sychev Dmitry A. 
Published: October 2018

AbstractIntroductionAlthough pharmacogenetic tests provide the information on a genotype and the predicted phenotype, these tests themselves do not provide the interpretation of data for a physician. There are currently approximately two dozen pharmacogenomic clinical decision support systems used in psychiatry. Implementation of clinical decision support systems capable of forming recommendations on drug and dose selection according to the results of pharmacogenetic testing is an urgent task. Fulfillment of this task may allow increasing the efficacy of therapy and decreasing the risk of undesirable side effects.Materials and methodsThe study included 51 male patients (21 in the main group and 30 in the control group) with alcohol withdrawal syndrome. To evaluate the efficacy and safety of therapy, several international psychometric scales and rating scales to measure side effects were used. Genotyping was performed using real‐time polymerase chain reaction with allele‐specific hybridization. Pharmacogenetic test results were interpreted using free software PGX2 (www.pgx2.com).ResultsStatistically significant differences between the scores derived from all psychometric scales were revealed. For instance, the total score on CIWA‐Ar scale by day 3 was 13.5 [11.2; 16.0] for the main group and 18.0 [17.0; 22.0] (p < 0.001) for the control group; by day 5, it was 6.5 [4.2; 8.0] for the main group and 15.0 [14.0; 16.0] (p < 0.001) for the control group. The UKU side effect rating scale (UKU) also revealed a statistically significant difference. The total score on UKU scale by day 3 was 6.0 [5.0; 7.0] for the main group and 7.0 [6.0; 8.0] (p < 0.001) for the control group; by day 5, this difference grew significantly: 5.5 [3.0; 9.0] for the main group and 14.0 [12.0; 19.0] (p < 0.001) for the control group. The groups were representative (there was no difference between the scores at the inclusion of patients).ConclusionPharmacogenetic‐guided personalization of drug dose in patients with alcohol withdrawal syndrome can reduce the risk of undesirable side effects and pharmacoresistance. It allows recommending the use of pharmacogenomic clinical decision support systems for optimizing drug dosage.
Recent Advances of Novel Proteinoids and Proteinoid Nanoparticles and Their Applications in Biomedicine and Industrial Uses
Authors: Kolitz‐Domb Michal , Margel Shlomo 
Published: October 2018

AbstractProteinoids, thermal polymers composed of amino acids, discovered and studied by Fox, spontaneously self‐assemble in spherical structures, microspheres, which Fox presented as the protocells of life. Fox's findings opened up a scope of applicable easy‐to‐make protein‐like particles. In recent years, interest in proteinoids has increased among nanobiomedicine research workers. These structures are suitable for biomedical applications, due to their protein‐like nature, biocompatibility, non‐toxicity and safety. Several new proteinoids made of a specific selection of amino acids were introduced for biomedical and agricultural industries. Several proteinoids include specific additives polymerized within their backbone, providing special chracteristics for a specific application. These proteinoids, their corresponding nanoparticles and their diverse applications are presented here, primarily focusing on proteinoids for cancer diagnostics and therapy, cosmetic and anti‐fog proteinoids.
Dual‐Targeted Supramolecular Vesicles Based on the Complex of Galactose Capped Pillar[5]Arene and Triphenylphosphonium Derivative for Drug Delivery
Authors: Shang Kun , Wang Yang , Lu Yuchao , Pei Zhichao , Pei Yuxin 
Published: October 2018

AbstractIn this study, a dual targeted supramolecular glycol‐vesicle based on the host‐guest complexation of galactose capped pillar[5]arene (GALWP5) and triphenylphosphonium derivative (D‐TPP) have been constructed (GALWP5⊃D‐TPP), which showed dual target potential (cell and mitochondria targeting) resulting from its galactose units and TPP units, respectively. The dual targeted glycol‐vesicle displays ignorable cytotoxicity and good mitochondria targeting. Our work presents a good example of rational design for an effective cell and subcellular organelles dual targeted delivery system.
How the intracellular partitioning of tRNA and tRNA modification enzymes affects mitochondrial function
Authors: Paris Zdeněk , Alfonzo Juan D. 
Published: October 2018

AbstractOrganisms have evolved different strategies to seclude certain molecules to specific locations of the cell. This is most pronounced in eukaryotes with their extensive intracellular membrane systems. Intracellular compartmentalization is particularly critical in genome containing organelles, which because of their bacterial evolutionary ancestry still maintain protein‐synthesis machinery that resembles more their evolutionary origin than the extant eukaryotic cell they once joined as an endosymbiont. Despite this, it is clear that genome‐containing organelles such as the mitochondria are not in isolation and many molecules make it across the mitochondrial membranes from the cytoplasm. In this realm the import of tRNAs and the enzymes that modify them prove most consequential. In this review, we discuss two recent examples of how modifications typically found in cytoplasmic tRNAs affect mitochondrial translation in organisms that forcibly import all their tRNAs from the cytoplasm. In our view, the combination of tRNA import and the compartmentalization of modification enzymes must have played a critical role in the evolution of the organelle. © 2018 IUBMB Life, 70(12):1207–1213, 2018
The influence of bone‐graft bio‐functionalization with plasma of argon on bacterial contamination
Authors: Genova Tullio , Pesce Paolo , Mussano Federico , Tanaka Kazushige , Canullo Luigi 
Published: October 2018

AbstractPlasma of argon was demonstrated to improve protein and cell adhesion on bone grafts. On the other hand, increased surface energy and hydrophilicity could potentially amplify the risks of graft surface contamination in a clinical environment. The aim of the present study was to in vitro verify if the plasma of argon treatment could alter the graft characteristics affecting its ability to remain sterile. Six graft materials produced by different company were selected for this study, and randomly split by allocation either in the test (Plasma of argon treatment for 20′) or the control group (only removed from the plastic sterile vials). To replicate the surgical work flow, both test and control samples were left 2 min in the clinical environment simulated conditions. Samples were therefore transferred in a Biosafety level 2 culture room. Bacterial growth analysis was performed. Optical density at 600 nm was measured as readout of bacterial growth and, after 24 hours, colony forming unit (CFU) was evaluated. Statistical analysis was performed by using the ordinary one‐way ANOVA. The optical density confirmed no significant differences within groups and the number of CFU/ml for each measured sample (test and control) failed to present significant differences. Data from the present study highlighted that surface activation using plasma of argon did not affect the degree of contamination of the bone grafts, allowing to maintain a required sterility of the surface. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 67–70, 2019.
In vitro degradation of a unique porous PHBV scaffold manufactured using selective laser sintering
Authors: Diermann Sven H. , Lu Mingyuan , Edwards Grant , Dargusch Matthew , Huang Han 
Published: October 2018

AbstractBiodegradable poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) scaffolds have shown great promise for bone tissue engineering applications. The investigation of their hydrolytic degradation is thus essential to understand the effect of hydrolysis on the complex biodegradation behavior of PHBV scaffolds. In this study, we investigated the degradation behavior of high molecular weight PHBV scaffolds manufactured using selective laser sintering (SLS) without using predesigned porous architectures. The manufactured scaffolds have high specific surface areas with great water‐uptake abilities. After an incubation of 6 weeks in phosphate‐buffered saline solution, the structural integrity of the scaffolds was unaffected. However, a significant decrease in molecular weight ranging from 39% to 46% was found. The measured weight loss was negligible, but their compressive modulus and strength both decreased, likely due to water plasticization. These findings suggest that hydrolytic degradation of PHBV by means of bulk degradation was the predominant mechanism, attributed to their excellent water absorptivity. Overall, the PHBV scaffolds manufactured using SLS exhibited adequate mechanical properties and satisfactory structural integrity after incubation. As a result, the scaffolds have great potential as candidates for bone repair in clinical practice. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 154–162, 2019.
The structure of (theta, pyramid, 1‐wheel, 3‐wheel)‐free graphs
Authors: Boncompagni Valerio , Radovanović Marko , Vušković Kristina 
Published: October 2018

AbstractIn this paper, we study the class of graphs defined by excluding the following structures as induced subgraphs: theta, pyramid, 1‐wheel, and 3‐wheel. We describe the structure of graphs in , and we give a polynomial‐time recognition algorithm for this class. We also prove that ‐free graphs in are 4‐colorable. We remark that includes the class of chordal graphs, as well as the class of line graphs of triangle‐free graphs.
Performance enhancement of ZnO‐based MSM photodiodes by optimizing structure parameters
Authors: Al‐Khalli Najeeb , Debbar Nacer 
Published: October 2018

AbstractA two‐dimensional numerical simulation based on the drift‐diffusion model has been used to study the influence of the structural parameters on the ZnO‐based thin film metal‐semiconductor‐metal photodiode's (MSM‐PD) optical performance. A comparative study on photodiodes (PDs) with different structural parameters (active layer thickness, finger spacing, and width) at different illumination and biasing conditions has been conducted. The results of the simulation disclose the limit of using thicker active layer and wider finger spacing to achieve superior responsivity. In fact, the responsivity rapidly saturates, and a significant degradation in the response time befalls on the performance of the PD. The investigation suggests an optimum structure for the MSM‐PD with about 0.2 μm active layer thickness, 5 μm finger spacing, and minimum achievable contact finger width.
Early Marine Isotope Stage 3 human occupation of the Shandong Peninsula, coastal North China
Authors: Li Feng , Wang Jian , Zhou Xinying , Wang Xiaomin , Long Hao , Chen Yupeng , Olsen John W. , Chen Fuyou 
Published: October 2018

ABSTRACTArcheological studies of coastal sites have yielded a large body of information regarding the dispersal of modern humans from Africa and the coastal adaptations of various hominin groups. Coastal areas have been attractive to humans since at least the late Middle Pleistocene, according to research conducted in Africa and the circum‐Mediterranean region. However, little information concerning Paleolithic occupations has come to light in coastal areas of China. Here, we report on the chronology, archeology and paleoenvironmental reconstruction of the Pleistocene Dazhushan site on the east coast of the Shandong Peninsula in North China. Evidence indicates that prehistoric humans employing a flake technology occupied the current coastal area of the peninsula by at least early Marine Isotope Stage 3 (MIS 3; c. 57–29 ka) when the region was an inland area dominated by a mixed broadleaved forest and grassland environment occupied by terrestrial herbivores. Based on archeological evidence brought to light along the current Chinese coastline, correlated with sea level changes that have occurred since MIS 3, we suggest that future studies of coastal migrations and adaptations in eastern China will be considerably enhanced by a deeper understanding of the geomorphological evolution of those coastal regions.
A database of Raman spectra of precious gemstones and minerals used as cut gems obtained using portable sequentially shifted excitation Raman spectrometer
Authors: Culka Adam , Jehlička Jan 
Published: October 2018

AbstractRaman spectra of 42 minerals, commonly and less frequently used as gemstones in gemmology s. s. or works of arts, have been recorded using a portable sequentially shifted excitation Raman spectrometer. Analyzed minerals include chrysoberyl, diamond, emerald, garnets, magnesiotaaffeite‐2N'2S (taaffeite), magnesiotaaffeite‐6N'3S (musgravite), ruby, topaz as well as other less precious minerals. Some minerals are represented by several different samples with various causes for fluorescence in order to ascertain the results of the fluorescence removal process on a larger set of natural mineralogical samples. This novel instrumentation is able to effectively suppress the laser‐induced fluorescence that is sometimes present in the Raman spectra of natural and often coloured samples of mostly silicate minerals, and at the same time allow the analyses of gemstones and works of arts in situ, which can be important in many cases.
Changes in the content of anthocyanins, flavonols, and antioxidant activity in Fragaria ananassa var. Camarosa fruits under traditional and organic fertilization
Authors: Ruiz Antonieta , Sanhueza Mario , Gómez Francisca , Tereucán Gonzalo , Valenzuela Tamara , García Susana , Cornejo Pablo , Hermosín‐Gutiérrez Isidro 
Published: October 2018

AbstractBackgroundStrawberries are consumed worldwide. In Chile, strawberry production has been established in Andisols, were phosphorus is scarce. Traditional fertilization (TF) and organic fertilization (OF) have both been established there. This study examined their impact on the polyphenolic content of strawberries.ResultsTwo anthocyanins were identified by high performance liquid chromatography with diode array detection coupled to mass spectrometry (HPLC‐DAD‐ESI‐MS/MS). The average total anthocyanin concentrations were found to be 651 mg kg−1 fresh weight in 100% OF, which represents a 56% increase compared to fruits that were not fertilized. In the case of flavonols, only quercetin‐rhamnoside was identified, and its concentration reached 14.6 mg kg−1 with 100% OF. The ascorbic acid concentration reached 0.54 g kg−1 in 50% TF (a 20% increase over fruits without fertilization, WF). The antioxidant activities slightly increased in the fruits subjected to TF and OF in comparison with WF treatment.ConclusionThese results support a management strategy for obtaining the best quality and potential beneficial effects in health by increasing anthocyanins and other polyphenols under OF. © 2018 Society of Chemical Industry
Advantage of liquid chromatography with high‐resolution mass spectrometry for the detection of the small and polar molecules trifluoroacetic acid and sulfamic acid
Authors: Seitz Wolfram , Schulz Wolfgang , Winzenbacher Rudi 
Published: October 2018

AbstractThis study addresses the selectivity of liquid chromatography with tandem mass spectrometry for the analysis of the polar analytes trifluoroacetic acid and sulfamic acid in water samples. Such analytes often lack sufficient retention in standard reversed‐phase liquid chromatography and rarely produce mass spectrometry transition products of high diagnostic evidence by collision‐induced dissociation. The chromatography based on an extra‐dense bonding liquid chromatography column for trifluoroacetic acid and on hydrophilic interaction chromatography for sulfamic acid. This enabled sufficient retention of more than twice the void volume for both analytes. Liquid chromatography coupled to low‐resolution mass spectrometry was compared with high‐resolution mass spectrometry. The limits of quantification for direct injection analysis were determined to <0.05 μg/L for trifluoroacetic acid and <0.5 μg/L for sulfamic acid. Correction of matrix effects either by internal standards or the standard addition is strongly recommended as matrix effects may vary strongly. The concentrations determined for trifluoroacetic acid and sulfamic acid for real samples were comparable between low and high‐resolution mass spectrometry. However, low‐resolution mass spectrometry does not fulfill the identification criteria of the analytes following the European Commission council directive 96/23/EC or the SANTE/11945/2015. Since low‐resolution mass spectrometry for these analytes may result in false‐positive findings, such findings may be controlled by high‐resolution mass spectrometry.
Separation of five naphtho‐γ‐pyrones from Pleurotus ostreatus by high‐speed countercurrent chromatography
Authors: Cai Xu , Yu Hongzhu , Yu Yang , Li Qian , Chen Benke , Huang Yun , Zou Xianwei , Huang Bisheng , Tang Jintian 
Published: October 2018

AbstractA method to optimize a high‐speed countercurrent chromatography solvent system from many possible solvent systems was guided by average polarity and K values of crude samples, which was successfully applied in separation of the naphtho‐γ‐pyrones. This method expanded the scope of spectrophotometer application in the solvent systems selection, and showed the merits of simplicity, rapidity, and low reagent consumption. As a consequence of utilizing the method, an efficient separation procedure was established, and two groups of naphtho‐γ‐pyrone isomers (fonsecinone B and asperpyrone F; asperpyrone C, fonsecinone A, and aurasperone A) were obtained from Pleurotus ostreatus by high‐speed countercurrent chromatography with a solvent system composed of petroleum ether/ethyl acetate/methanol/water (5:5:5:5, v/v). The results indicate that high‐speed countercurrent chromatography could be a powerful technology for separation isomers and analogues, and the method guided by average polarity and K values of crude samples is feasible to optimize solvent system.