bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Gliding Brow Lift: An Analysis of Outcomes and Maintenance of Lift in a Single Surgeon's Practice
Authors: Orra Susan , Bald Madeline , Wagner Grant , Grotting James C 
Published: November 2024

Abstract

Background
Efforts in brow lifting longevity, desire for minimally invasive procedures, patient individualization, and minimization of complications led to the development of the gliding brow lift (GBL). The GBL has proven to be a successful, minimally invasive technique targeting brow elevation in combination with the hemostatic net.

Objectives
The aim of this study was to evaluate maintenance of cutaneous brow elevation with the GBL technique at specific postoperative time points.

Methods
A retrospective review was conducted of 63 patients (59 females, 4 males) who underwent a GBL by J.C.G. between 2019 and 2022 for comorbidities, maintenance of brow lift, and incidence of complications at 4 postoperative time points: 3, 6, 9, and 12 months. Preoperative and postoperative photographs were analyzed at 4 anatomic locations: the supraorbital rim, lateral canthus, midpupil, and medial canthus.

Results
The cohort consisted of 59 female-only patients; male patients were excluded due to small sample size. The average age was 62.8 years old (SD ±7.01) and the mean BMI was 24. Only 1 patient required return to the operating room for hematoma evacuation; this was a result of a concomitant rhytidectomy and did not involve the brow. All locations maintained a statistically significant lift (P &lt; .01). The most significant lift was at the lateral canthus (3.09 mm) and tail of the brow (2.74 mm) at 1 year postoperatively.

Conclusions
The GBL is a successful innovation in aesthetic brow surgery. Depending on patient needs, it is an excellent choice for selective and long-term elevation of the lateral brow.

Level of Evidence: 4 (Therapeutic)
GGN-GO: geometric graph networks for predicting protein function by multi-scale structure features
Authors: Mi Jia , Wang Han , Li Jing , Sun Jinghong , Li Chang , Wan Jing , Zeng Yuan , Gao Jingyang 
Published: November 2024

Abstract
Recent advances in high-throughput sequencing have led to an explosion of genomic and transcriptomic data, offering a wealth of protein sequence information. However, the functions of most proteins remain unannotated. Traditional experimental methods for annotation of protein functions are costly and time-consuming. Current deep learning methods typically rely on Graph Convolutional Networks to propagate features between protein residues. However, these methods fail to capture fine atomic-level geometric structural features and cannot directly compute or propagate structural features (such as distances, directions, and angles) when transmitting features, often simplifying them to scalars. Additionally, difficulties in capturing long-range dependencies limit the model’s ability to identify key nodes (residues). To address these challenges, we propose a geometric graph network (GGN-GO) for predicting protein function that enriches feature extraction by capturing multi-scale geometric structural features at the atomic and residue levels. We use a geometric vector perceptron to convert these features into vector representations and aggregate them with node features for better understanding and propagation in the network. Moreover, we introduce a graph attention pooling layer captures key node information by adaptively aggregating local functional motifs, while contrastive learning enhances graph representation discriminability through random noise and different views. The experimental results show that GGN-GO outperforms six comparative methods in tasks with the most labels for both experimentally validated and predicted protein structures. Furthermore, GGN-GO identifies functional residues corresponding to those experimentally confirmed, showcasing its interpretability and the ability to pinpoint key protein regions. The code and data are available at: https://github.com/MiJia-ID/GGN-GO
DEWNA: dynamic entropy weight network analysis and its application to the DNA-binding proteome in A549 cells with cisplatin-induced damage
Authors: Wang Shisheng , Zeng Wenjuan , Yang Yin , Cheng Jingqiu , Liu Dan , Yang Hao 
Published: November 2024

Abstract
Cisplatin is one of the most commonly used chemotherapy drugs for treating solid tumors. As a genotoxic agent, cisplatin binds to DNA and forms platinum-DNA adducts that cause DNA damage and activate a series of signaling pathways mediated by various DNA-binding proteins (DBPs), ultimately leading to cell death. Therefore, DBPs play crucial roles in the cellular response to cisplatin and in determining cell fate. However, systematic studies of DBPs responding to cisplatin damage and their temporal dynamics are still lacking. To address this, we developed a novel and user-friendly stand-alone software, DEWNA, designed for dynamic entropy weight network analysis to reveal the dynamic changes of DBPs and their functions. DEWNA utilizes the entropy weight method, multiscale embedded gene co-expression network analysis and generalized reporter score-based analysis to process time-course proteome expression data, helping scientists identify protein hubs and pathway entropy profiles during disease progression. We applied DEWNA to a dataset of DBPs from A549 cells responding to cisplatin-induced damage across 8 time points, with data generated by data-independent acquisition mass spectrometry (DIA-MS). The results demonstrate that DEWNA can effectively identify protein hubs and associated pathways that are significantly altered in response to cisplatin-induced DNA damage, and offer a comprehensive view of how different pathways interact and respond dynamically over time to cisplatin treatment. Notably, we observed the dynamic activation of distinct DNA repair pathways and cell death mechanisms during the drug treatment time course, providing new insights into the molecular mechanisms underlying the cellular response to DNA damage.
Inhibition of phospholipase D1 reduces pancreatic carcinogenesis in mice partly through a FAK-dependent mechanism
Authors: Addassi Hala A , Krga Irena , Villarreal Fernando , LaComb Joseph F , Frohman Michael A , Matsukuma Karen , Mackenzie Gerardo G 
Published: November 2024

Abstract
Phospholipase D (PLD) plays a critical role in cancer progression. However, its role in pancreatic cancer remains unclear. Thus, we evaluated the role of PLD1, one of two classical isoforms of PLD, in pancreatic carcinogenesis in vivo. The role of PLD1 in tumor growth was evaluated by subcutaneously transplanting human MIA PaCa-2 cells expressing endogenous PLD1 levels (Ctr KD cells) or cells in which PLD1 was knocked down (Pld1 KD cells) into immunodeficient mice. Twenty days post-implantation, tumors that arose from Pld1-KD cells were significantly smaller, compared to controls (Ctr KD). Then, we assessed the role of PLD1 in the tumor microenvironment, by subcutaneously implanting mouse LSL-KrasG12D/+;Trp53R172H/+;Pdx-1-Cre (KPC) cells into wild-type or PLD1 knockout (Pld1−/−) mice. Compared to wild type, tumor growth was attenuated in Pld1−/− mice by 39%, whereas treatment of Pld1−/− mice with gemcitabine reduced tumor growth by 79%. When PLD1 was ablated in LSL-KrasG12D;Ptf1Cre/+ (KC) mice, no reduction in acinar cell loss was observed, compared to KC mice. Finally, treatment of KC mice with a small molecule inhibitor of PLD1 and PLD2 (FIPI) significantly reduced acinar cell loss and cell proliferation, compared to vehicle-treated mice. Mechanistically, the effect of PLD on tumor growth is mediated, partly, by the focal adhesion kinase pathway. In conclusion, while PLD1 is a critical regulator of pancreatic xenograft and allograft growth, playing an important role at the tumor and at the microenvironment levels, the inhibition of PLD1 and PLD2 is necessary to reduce pancreatic carcinogenesis in KC mice and might represent a novel therapeutic target.
Impact of Histological Remission for Predicting Clinical Relapse in Crohn’s Disease: A Post Hoc Analysis of the Prospective STORI Cohort
Authors: Reenaers Catherine , Enea Diana , Nachury Marie , Laharie David , Bouhnik Yoram , Fumery Mathurin , Gornet Jean-Marc , Amiot Aurélien , Altwegg Romain , de Vos Martine , Marteau Philippe , Bourreille Arnaud , Nancey Stéphane , Viennot Stéphanie , Louis Edouard , Svrcek Magali 
Published: November 2024

Abstract

Background and aims
Achieving deep remission, encompassing clinical, endoscopic, and biological remission, is the goal in managing Crohn’s disease (CD). The role of histological remission (HR) remains unclear. This study aimed to examine the impact of histological inflammation on clinical relapse risk in CD and explore the relationship between histology, endoscopic scores, and biomarkers.

Methods
Patients from the prospective STORI (Stable Remission on Combined Therapy with Immunosuppressors) cohort underwent ileocolonoscopy with Crohn’s Disease Endoscopic Index of Severity calculation and 2 biopsies from the most inflamed or previously inflamed areas. Histological scores (Robarts, Geboes, modified Geboes, Nancy, and IBD-DCA) were determined by 2 independent pathologists in a central reading process. Histological remission was defined by specific score thresholds. Clinical relapse, defined by Crohn’s Disease Activity Index (CDAI) &gt; 250 or a CDAI increase of 70 points over 2 weeks, was monitored for at least 1 year.

Results
Out of 115 patients included in STORI, 160 biopsies (44 ileal and 116 colonic) from 76 patients were analyzed. Histological remission rates were 46% (Nancy), 55% (Robarts), 61% (Geboes), and 41% (IBD-DCA). During follow-up, 35 patients (46%) experienced a clinical relapse: 37% with HR and 56% without, based on the Nancy score. Among the mucosal healing subgroup (45 patients), 34% with HR, and 44% without relapsed (p = 0.18). Histological scores did not predict clinical relapse. Only fecal calprotectin was a significant predictor in multivariate analysis (p = 0.029).

Conclusions
Despite correlations with endoscopy and biomarkers, histological scores did not predict clinical relapse in CD patients in remission. Thus, these scores are not recommended for clinical practice to assess relapse risk in CD.
Electrophysiological tolerance: a new concept for understanding the electrical stability of the heart
Authors: Stokke Mathis K , Louch William E , Smith Godfrey L 
Published: November 2024

Abstract
The co-ordinated electrical activity of ∼2 billion cardiac cells ensures stability of the heartbeat. Indeed, the remarkably low incidence (&lt;1%) of ventricular arrhythmias in the healthy heart is only possible when the electrical event across this syncytium is closely controlled. In contrast, the diseased myocardium is associated with increased electrophysiological heterogeneity, unstable rhythm, and increased incidence of lethal arrhythmias. But what is the link between cellular and tissue level heterogeneity? Recent research has shown the existence of considerable cellular heterogeneity even in the healthy heart, suggesting that cell-to-cell variability in electrical (e.g. action potential duration) and mechanical performance (e.g. twitch amplitude) is a normal property. This observation has been previously unappreciated because the aggregated function in the form of QT-interval and cardiac output varies &lt;1% on a beat-to-beat basis. This article describes the underlying cellular variability that is tolerated—and perhaps needed—by different regions of the heart for normal function and indicates why this variability is not apparent in function at the chamber and organ level. Thus, in contrast to the current dominant view, this article postulates that heterogeneity is normal and potentially endows various functional benefits. This new view of how the component parts of the heart come together to function also suggests novel mechanisms for cardiac pathologies, namely that dysfunction may emerge from changes in the extent and/or nature of heterogeneity. Once understood, restoring normal forms of heterogeneity could be a novel approach to treatment.
Digest: Extremes of the mating system continuum are the most evolutionarily stable
Authors: Luzuriaga-Aveiga Vanessa E 
Published: November 2024

Abstract
Marcondes and Douvas [(2024). Social mating systems in birds: Resource-defense polygamy—but not lekking—is a macroevolutionarily unstable trait. Evolution, qpae123] provide a macroscale insight into the evolution of mating systems in birds. The study shows that resource-defense polygamous lineages are evolutionarily unstable, commonly transitioning into monogamy or going extinct. Surprisingly, lek polygamy is as evolutionary stable as monogamy. While in monogamy both sexes benefit from cooperative behavior by equally investing in parental care, in lekking systems each sex maximizes their fitness independently: females invest in parental care and males enhance sexually selected traits.
Digital Contact as Strain or Support: How Does Type of Contact Shape the Association Between Mother–Child Interactions and Adult Children’s Depressive Symptoms in Later-Life Families?
Authors: He Ranran , Suitor J Jill , Gilligan Megan 
Published: November 2024

Abstract

Background and Objectives
This paper aims to extend research on the association between mother–child contact and adult children’s psychological well-being in later-life families by differentiating between in-person, virtual, and written digital contact, examining the moderating role of children’s gender, and exploring the processes that underlie these associations.

Research Design and Methods
Mixed-methods data were collected from 250 adult children nested within 131 families as part of the third wave of the Within-Family Differences Study. On average, adult children were 59 and mothers were 88 years of age.

Results
Multilevel analyses revealed that interacting with mothers through written digital media was associated with higher depressive symptoms among adult children, whereas mother–child in-person and virtual digital contact were not associated with children’s depressive symptoms. When stratifying the sample by children’s gender, we found that mother–child written digital contact was associated with higher depressive symptoms among daughters, but not sons. Qualitative analyses suggested that adult daughters’ psychological well-being was negatively associated with written digital contact with their mothers because this medium of interaction typically fell short of daughters’ expectations for emotionally enriching exchanges.

Discussion and Implications
This study revealed differences in how virtual and written digital contact are associated with psychological well-being among adult daughters, but not sons, suggesting that it is important to consider type of contact, gender of interactants, and content of exchanges when studying the associations between digital communication and relational and psychological well-being and designing intervention programs and digital communication technologies.
Alternative polyadenylation shapes the molecular and clinical features of lung adenocarcinoma
Authors: Gao Yipeng , Shaw Vikram R , Amos Christopher I 
Published: November 2024

Abstract
Alternative polyadenylation (APA) is a major mechanism of post-transcriptional regulation that affects mRNA stability, localization and translation efficiency. Previous pan-cancer studies have revealed that APA is frequently disrupted in cancer and is associated with patient outcomes. Yet, little is known about cancer type-specific APA alterations. Here, we integrated RNA-sequencing data from a Korean cohort (GEO: GSE40419) and The Cancer Genome Atlas (TCGA) to comprehensively analyze APA alterations in lung adenocarcinomas (LUADs). Comparing expression levels of core genes involved in polyadenylation, we find that overall, the set of 28 of 31 genes are upregulated, with CSTF2 particularly upregulated. We observed broad and recurrent APA changes in LUAD growth-promoting genes. In addition, we find enrichment of APA events in genes associated with known LUAD pathways and an increased heterogeneity in polyadenylation (polyA) site usage of proliferation-associated genes. Upon further investigation, we report smoking-specific APA changes are also highly relevant to LUAD development. Overall, our in-depth analysis reveals APA as an important driver for the molecular and clinical features of lung adenocarcinoma.
Patent opposition, IP firm capabilities, and technology entry: empirical evidence from European patent data
Authors: Martinelli Arianna , Mazzei Julia , Moschella Daniele 
Published: November 2024

Abstract
This paper examines the extent to which patent litigiousness relates to entry into a new technological domain (TD). Unless firms have sufficient intellectual property (IP) capabilities, patent litigiousness in the form of opposition may raise barriers to entry. We test this hypothesis using a large-scale dataset of patents applied for at the European Patent Office (EPO) between 2000 and 2015. We introduce a new measure of TD patent litigiousness based on the frequency of oppositions at the EPO. Patent litigiousness is negatively associated with technological entry for small firms, especially in high-tech and complex TDs. Unlike small firms, large firms benefit from previous experience with opposition, which favors the development of IP capabilities and reduces the uncertainty of entering highly litigious TDs.
Dos ejes para pensar la tensión entre el sistema interamericano de derechos humanos y los sistemas constitucionales de la regiónTwo axes to consider the tension between the Inter-American System of Human Rights and the Constitutional systems of the region
Authors: Maisley Nahuel 
Published: November 2024

Abstract
The dispute over the authority to interpret the American Convention on Human Rights has generally been conceptualized along a single axis, with the Inter-American Court at one end and States at the other. This article suggests that the tension should be analyzed considering, instead, two perpendicular and independent conceptual axes. First, a geographic or vertical axis, in which competencies are distributed between geographically and jurisdictionally overlapping political communities (the national and inter-American political community, respectively). And second, an institutional or horizontal axis, in which competencies are distributed among institutional bodies (legislatures and courts). These two axes produce a Cartesian scheme of coordinates, useful to map the various positions existing in academic, judicial, and political debates and to highlight the genuine normative complexity underlying the disputes. The article concludes suggesting a research agenda structured around imagining how to expand instances of regional democratic governance within the system.
Taxon-specific contributions of microeukaryotes to biological carbon pump in the Oyashio region
Authors: Yang Qingwei , Yang Yanhui , Xia Jun , Fukuda Hideki , Okazaki Yusuke , Nagata Toshi , Ogata Hiroyuki , Endo Hisashi 
Published: November 2024

Abstract
Microeukaryotes are critical components of sinking particles contributing to carbon export from the surface to deep oceans. However, the knowledge of the sinking microeukaryotic communities and their dynamics is currently limited. In this study, we applied 18S rRNA gene metabarcoding to investigate the microeukaryotic communities in sinking and suspended particles distinguished by marine snow catchers during spring in the Oyashio region. Sinking particles displayed distinct communities and lower diversity than suspended particles. The community compositions of the sinking particles varied with depth, suggesting that microeukaryotes were selectively disaggregated or decomposed during settling. Prymnesiophyceae and diatoms were effectively removed, as indicated by their decreased abundance in sinking particles at increasing depths. Conversely, Dinophyceae maintained a higher abundance in sinking particles across depths, indicating resistance to disaggregation and decomposition. Spirotrichea and heterotrophic Dinophyceae were enriched in sinking particles, while marine stramenopiles groups were enriched in suspended particles. The heterotrophs in the deeper layers were mainly transported from the surface layers by increasing their relative abundance towards deep layers, indicating that they contributed to the transformation processes of sinking particles. Overall, our results demonstrate the functional differences among microeukaryotes in the biological carbon pump.
Evaluation of guanidinoacetic acid supplementation on finishing beef steer growth performance, skeletal muscle cellular response, and carcass characteristics
Authors: Eckhardt Erika P , Kim Wonseob , Jaborek Jerad , Garmyn Andrea J , Kang Donghun , Kim Jongkyoo 
Published: November 2024

Abstract
This study elucidated the effects of dosage-dependent guanidinoacetic acid (GAA) supplementation on growth performance, muscle responses, and carcass characteristics in finishing beef steers. Thirty crossbred Red Angus beef steers (395 ± 28.09 kg) were randomly assigned one of three treatments during a 146-d feedlot study: basal diet without GAA supplementation (CONTROL), 1g of GAA per 100 kg of body weight (BW) daily (LOWGAA), and 2 g of GAA per 100 kg of BW daily (HIGHGAA). Individual feed intake was monitored daily, growth performance parameters were collected every 28 d, and longissimus muscle (LM) biopsies occurred every 56 d. In biopsied LM, greater (P = 0.048) mRNA expression of IGF-1 was observed in LOWGAA steers on day 112 compared to the CONTROL group. LOWGAA steers also exhibited greater expression of myosin heavy chain (MHC) I compared to CONTROL steers (P &lt; 0.05) and MHC IIA compared to both CONTROL and HIGHGAA treatment groups (P &lt; 0.01) on day 112. GAA supplementation resulted in no change in carcass characteristics, serum and LM tissue metabolites, LM composition, and Warner–Bratzler shear force values (P &gt; 0.05). Data collected from this study demonstrate the influence of GAA supplementation on the gene expression of MHC isoforms and their role in skeletal muscle growth, differentiation, and muscle fiber-typing.
The Advaita Vedānta Monastic Tradition of Nampūtiri Brahmins in Kerala: Tracing the Order’s History in the Inscriptional Evidence
Authors: Nowicka Olga 
Published: November 2024

Abstract
This article attempts to reconstruct the origins of the Nampūtiri Advaita Vedānta maṭhas in Kerala, which constitute an independent vernacular variant of Śaṅkaran monasticism. The early history of the Nampūtiri monastic centres of Kerala still remains very much obscure. This article aims to fill the lacuna and propose a working hypothesis of order in question, tracing its sources to the local institution of a ‘temple saṃnyāsin’, referred to in the Keralan epigraphs as bhaṭāra-tiruvaṭi or paḻāra-tiruvaṭi. In order to reconstruct the early history of the local monastic tradition, I focus primarily on the available inscriptional material, which I discuss at length in the latter part of the article, that follows the elaborate description of the vernacular sampradāya based on the ethnographic material, constituting foundation for integrating the epigraphic evidence.