bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synovial sarcoma of the supraclavicular region causing a hemorrhagic shock
Authors: Halily Sara , Ballage Amine , Ardhaoui Hamida , Rouadi Sami , Abada Redallah , Roubal Mohamed , Mahtar Mohamed , El Khiraoui Hasna , Belhaj Salwa , Marnissi Farida 
Published: September 2019

AbstractA cystic mass that rapidly increases in volume in a dangerous anatomical region, notably the supraclavicular region, which contains important neurovascular elements, must be considered at high risk. A vascular study is mandatory before biopsy. Neoadjuvant chemotherapy can be a treatment option in high‐risk synovial sarcoma.
Oxygen Reduction Investigation on Sputtered ZnO Layers with Nano‐granular Structure
Authors: Soares Costa Josiane , Prestat Michel , Lescop Benoit , Rioual Stéphane , Tribollet Bernard , Holzer Lorenz , Pélissier Krystel , Thierry Dominique 
Published: September 2019

AbstractZinc oxide (ZnO) films with nano‐granular structure were deposited by radio‐frequency magnetron sputtering. The absence of preferred orientation was verified by XRD. HAADF‐STEM images revealed a nanoporous structure with pore and grain sizes around 10 nm. The narrowness of the pores enabled to approximate that oxygen reduction takes place at the surface of the layers. During cathodic polarization, the surface of the electrodes became rougher due to the development of nodules on the surface. The ZnO electrodes exhibit a high activity towards oxygen reduction in KOH solution (pH=10). Rotating ring‐disk electrode measurements coupled with reaction modelling allowed to quantitatively demonstrate that the direct reduction prevailed with the indirect pathway being active as well.
5,5,15,15‐Tetraoxo‐5,15‐Dithiaporphyrin as a Highly Electron‐Deficient Porphyrinic Ligand
Authors: Yagi Atsumi , Kondo Takeshi , Yamashita Daisuke , Wachi Naruhiko , Omori Hiroto , Fukui Norihito , Ikeue Takahisa , Shinokubo Hiroshi 
Published: September 2019

AbstractOxidation of 5,15‐dithiaporphyrin with meta‐chloroperbenzoic acid afforded the corresponding S,S‐tetraoxide in good yield. The resultant 5,5,15,15‐tetraoxo‐5,15‐dithiaporphyrin exhibited the highly electron‐deficient nature as elucidated by the electrochemical analysis and theoretical calculations. Treatment of tetraoxodithiaporphyrin with zinc(II) acetate and nickel(II) acetate provided the corresponding metal complexes efficiently. Owing to its enhanced Lewis acidity of the metal center by the electron‐deficient ligand, the nickel complex underwent facile axial ligation to form pentacoordinate and hexacoordinate high‐spin (S=1) complexes in solution and solid, respectively. The binding constant of pyridine to the NiII center was significantly higher than those of conventional porphyrin NiII complexes. Temperature‐dependent magnetic susceptibility measurements of the high‐spin NiII complex revealed the presence of weak ferromagnetic interactions.
Visible‐Light‐Driven Intermolecular Reductive Ene–Yne Coupling by Iridium/Cobalt Dual Catalysis for C(sp3)−C(sp2) Bond Formation
Authors: González María J. , Breit Bernhard 
Published: September 2019

AbstractA new methodology to form C(sp3)−C(sp2) bonds by visible‐light‐driven intermolecular reductive ene–yne coupling has been successfully developed. The process relies on the ability of the Hantzsch ester to contribute in both SET and HAT processes through a unified cobalt and iridium catalytic system. This procedure avoids the use of stoichiometric amounts of reducing metallic reagents, which is translated into high functional‐group tolerance and atom economy.
Towards Extended Zinc Ethylsulfinate Networks by Stepwise Insertion of Sulfur Dioxide into Zn−C Bonds
Authors: Tulewicz Adam , Wolska‐Pietkiewicz Małgorzata , Jędrzejewska Maria , Ratajczyk Tomasz , Justyniak Iwona , Lewiński Janusz 
Published: September 2019

AbstractInvited for the cover of this issue are the groups of Janusz Lewiński from Polish Academy of Sciences and Warsaw University of Technology. The image depicts how a zinc ethylsulfinate web can be woven by using diethylzinc and sulfur dioxide. Read the full text of the article at 10.1002/chem.201902733.
MicroRNA‐210 Promotes Bile Acid–Induced Cholestatic Liver Injury by Targeting Mixed‐Lineage Leukemia‐4 Methyltransferase in Mice
Authors: Kim Young‐Chae , Jung Hyunkyung , Seok Sunmi , Zhang Yang , Ma Jian , Li Tiangang , Kemper Byron , Kemper Jongsook Kim 
Published: September 2019

Background and Aims
Bile acids (BAs) are important regulators of metabolism and energy balance, but excess BAs cause cholestatic liver injury. The histone methyltransferase mixed‐lineage leukemia‐4 (MLL4) is a transcriptional coactivator of the BA‐sensing nuclear receptor farnesoid X receptor (FXR) and epigenetically up‐regulates FXR targets important for the regulation of BA levels, small heterodimer partner (SHP), and bile salt export pump (BSEP). MLL4 expression is aberrantly down‐regulated and BA homeostasis is disrupted in cholestatic mice, but the underlying mechanisms are unclear.

Approach and Results
We examined whether elevated microRNA‐210 (miR‐210) in cholestatic liver promotes BA‐induced pathology by inhibiting MLL4 expression. miR‐210 was the most highly elevated miR in hepatic SHP‐down‐regulated mice with elevated hepatic BA levels. MLL4 was identified as a direct target of miR‐210, and overexpression of miR‐210 inhibited MLL4 and, subsequently, BSEP and SHP expression, resulting in defective BA metabolism and hepatotoxicity with inflammation. miR‐210 levels were elevated in cholestatic mouse models, and in vivo silencing of miR‐210 ameliorated BA‐induced liver pathology and decreased hydrophobic BA levels in an MLL4‐dependent manner. In gene expression studies, SHP inhibited miR‐210 expression by repressing a transcriptional activator, Kruppel‐like factor‐4 (KLF4). In patients with primary biliary cholangitis/cirrhosis (PBC), hepatic levels of miR‐210 and KLF4 were highly elevated, whereas nuclear levels of SHP and MLL4 were reduced.

Conclusions
Hepatic miR‐210 is physiologically regulated by SHP but elevated in cholestatic mice and patients with PBC, promoting BA‐induced liver injury in part by targeting MLL4. The miR‐210–MLL4 axis is a potential target for the treatment of BA‐associated hepatobiliary disease.
Role of forkhead box gene family in bone metabolism
Authors: Huang Jinjing , Shen Gengyang , Ren Hui , Zhang Zhida , Yu Xiang , Zhao Wenhua , Shang Qi , Cui Jianchao , Yu Peiyuan , Peng Jiancheng , Liang De , Yang Zhidong , Jiang Xiaobing 
Published: September 2019

AbstractBone metabolism is associated with many bone diseases and regulated by multiple signal pathways. Over the past three decades, the functions of a superfamily of evolutionarily conserved transcriptional regulators, known as forkhead box (Fox) family, has been demonstrated to contribute to the bone metabolism. Genetic analysis studies have demonstrated that Fox gene family participate in bone metabolism and that their expression can be regulated by multiple factors. The deregulation of Fox gene family can lead to a series of bone metabolic diseases. In this manuscript, we sketched the biology of the Foxs family, summarized its function of regulating bone metabolism and maintaining bone homeostasis to estimate its potential therapeutic effects in bone diseases, and suggested directions for future exploration in this important field.
Droplet‐digital PCR assay to detect Merkel cell polyomavirus sequences in chorionic villi from spontaneous abortion affected females
Authors: Tagliapietra Andrea , Rotondo John Charles , Bononi Ilaria , Mazzoni Elisa , Magagnoli Federica , Gonzalez Lucia Oton , Contini Carlo , Vesce Fortunato , Tognon Mauro , Martini Fernanda 
Published: September 2019

AbstractDroplet‐digital polymerase chain reaction (ddPCR) technique was set up to detect/quantify Merkel cell polyomavirus (MCPyV) DNA in clinical specimens, including chorionic villi and peripheral blood mononuclear cells (PBMCs) from spontaneous abortion (SA)‐affected females. This ddPCR assay showed high accuracy, sensitivity, and specificity in detecting MCPyV DNA cloned in a recombinant plasmid vector, the control. ddPCR was extended to MCPyV DNA to investigate/quantify its sequences in clinical samples. Overall, 400 samples were analyzed, that is, 100 chorionic villi and 100 PBMCs, from SA females (n = 100), the cases, and 100 chorionic villi and 100 PBMCs from females who underwent voluntary pregnancy interruption (VI, n = 100), the control. MCPyV DNA was detected in 4/100 (4%) and 5/100 (5%) of SA and VI chorionic villi, respectively. The mean viral DNA load was 1.99 ( ± 0.94 standard mean deviation [SD]) copy/104 cells in SA and 3.02 ( ± 1.86 [SD]) copy/104 cells in VI. In PBMCs, MCPyV DNA was revealed in 9/100 (9%) and 14/100 (14%) of SA and VI, with a mean of 2.09 ( ± 1.17 [SD]) copy/104 cells and 4.09 ( ± 4.26 [SD]) copy/104 cells in SA and VI, respectively. MCPyV gene expression analysis by quantitative PCR for the large T antigen (LT) and viral capsid protein 1 (VP1) showed their mRNAs in 2/4 (50%) SA‐ and 2/5 (40%) VI‐MCPyV‐positive samples. MCPyV DNA was detected/quantified using the ddPCR technique, in chorionic villi and PBMCs from SA and VI. In our experimental conditions, ddPCR provided a powerful tool to detect/quantify MCPyV DNA sequences in clinical samples.
Nrf2–ARE signaling in cellular protection: Mechanism of action and the regulatory mechanisms
Authors: Shaw Pallab , Chattopadhyay Ansuman 
Published: September 2019

AbstractOxidative stress is the increase in cellular oxidant concentration in comparison to antioxidant titer. Toxic insults and many other diseased conditions are mediated through the formation of such condition. Once the redox equilibrium is disrupted, the cellular antioxidant system functions to bring back the cell to redox homeostasis state. The field players of the cytoprotective machinery are the xenobiotic‐metabolizing enzymes that are transcriptionally controlled by upstream regulatory pathways like the Nrf2–ARE pathway and AhR–XRE pathway. The importance of Nrf2 lies in the fact that it is activated by a variety of compounds and has a wide range of inducers including metals, organic toxicants and so forth. The present review article aims to discuss the role of Nrf2 in cellular protection and also intends to illuminate the regulatory mechanisms that control Nrf2 itself. This can add to our knowledge of how the cell reacts and survives against such stressed conditions.
Isolation and phenotypic characterization of tumor cells of patients with a diagnosis of ovarian cancer
Authors: Gutiérrez‐Castañeda Luz D. , Tovar‐Parra David , Quintero Gloria , Amezquita Lorena , Guerrero Carlos , Sanabria Daniel 
Published: September 2019

AbstractOvarian cancer is the fifth leading cause of cancer‐related deaths. It causes approximately 125,000 deaths per year worldwide; its diagnosis is made in advanced stages resulting in a high mortality rate. The objective of the study was optimizing the isolation of cells obtained from the solid tumor and ascitic fluid of patients with ovarian cancer and the phenotype with markers related to the epithelial–mesenchymal transition. For this, the solid tumor tissue was disaggregated and cultivated with different methodologies. As a result, cell growth was obtained and epi‐immunofluorescence was performed using antibodies against E‐cadherin, EpCAM, N‐cadherin, vimentin, CD133, and CD44. The primary culture from the solid tumor was obtained using Dispase II and DMEM/F12. Finally, heterogeneity was detected in terms of the expression of mesenchymal and epithelial type markers in the two types of isolated cells. Additionally, CD133 and CD44 expression was detected, proteins associated with the tumor stem cells phenotype.
lncrps25 play an essential role in motor neuron development through controlling the expression of olig2 in zebrafish
Authors: Gao Tianheng , Li Jingyun , Li Nan , Gao Yan , Yu Lingling , Zhuang Sisi , Zhao Yingmin , Dong Xiaohua 
Published: September 2019

Abstractlncrps25 is an intergenic long noncoding RNA (lncRNA), which is location close to rps25 (ribosomal protein S25) gene, is reported share high conserved sequence with NREP (neuronal regeneration‐related protein) 3′‐untranslated region. The function and mechanism of most of the lncRNA in embryo development remain largely unknown. In zebrafish, lncrps25 is widely expressed in the early embryonic stage and spinal cord during development. Morpholino (MO) knockdown of zebrafish lncrps25 exhibit locomotor behavior defects, caused by abnormal development of motor neurons. In addition, the defect of swimming ability and motor neurons could be recovery by microinject with lncrps25 RNA in lncrps25 morphants. By performing RNA sequencing and quantitative real‐time polymerase chain reaction, we found that olig2 (oligodendrocyte transcription factor 2) messenger RNA (mRNA) was downregulated in lncrps25 morphants. Moreover, overexpression of olig2 mRNA in lncrps25 morphants partially rescued motor neurons development. Taken together, these results indicate that lncrps25 plays an essential role in the development of motor neurons in zebrafish.
Patient assessment of chatbots for the scalable delivery of genetic counseling
Authors: Schmidlen Tara , Schwartz Marci , DiLoreto Kristy , Kirchner H. Lester , Sturm Amy C. 
Published: September 2019

AbstractA barrier to incorporating genomics more broadly is limited access to providers with genomics expertise. Chatbots are a technology‐based simulated conversation used in scaling communications. Geisinger and Clear Genetics, Inc. have developed chatbots to facilitate communication with participants receiving clinically actionable genetic variants from the MyCode® Community Health Initiative (MyCode®). The consent chatbot walks patients through the consent allowing them to opt to receive more or less detail on key topics (goals, benefits, risks, etc.). The follow‐up chatbot reminds participants of suggested actions following result receipt and the cascade chatbot can be sent to at‐risk relatives by participants to share their genetic test results and facilitate cascade testing. To explore the acceptability, usability, and understanding of the study consent, post‐result follow‐up and cascade testing chatbots, we conducted six focus groups with MyCode® participants. Sixty‐two individuals participated in a focus group (n = 33 consent chatbot, n = 29 follow‐up and cascade chatbot). Participants were mostly female (n = 42, 68%), Caucasian (n = 58, 94%), college‐educated (n = 33,53%), retirees (n = 38, 61%), and of age 56 years or older (n = 52, 84%). Few participants reported that they knew what a chatbot was (n = 10, 16%), and a small number reported that they had used a chatbot (n = 5, 8%). Qualitative analysis of transcripts and notes from focus groups revealed four main themes: (a) overall impressions, (b) suggested improvements, (c) concerns and limitations, and (d) implementation. Participants supported using chatbots to consent for genomics research and to interact with healthcare providers for care coordination following receipt of genomic results. Most expressed willingness to use a chatbot to share genetic information with relatives. The consent chatbot presents an engaging alternative to deliver content challenging to comprehend in traditional paper or in‐person consent. The cascade and follow‐up chatbots may be acceptable, user‐friendly, scalable approaches to manage ancillary genetic counseling tasks.
Heterologous expression of the puuE from Oenococcus oeni SD‐2a in Lactobacillus plantarum WCFS1 improves ethanol tolerance
Authors: Yuan Lin , Zhao Hongyu , Liu Longxiang , Peng Shuai , Li Hua , Wang Hua 
Published: September 2019

AbstractOenococcus oeni is the main bacteria extensively used in malolactic fermentation due to its high tolerance against stress factors in wine production. Among these, ethanol is one of the main challenges to O. oeni, and its ethanol tolerance mechanism remains unclear. In this study, the puuE gene related to ethanol tolerance from O. oeni SD‐2a was heterologously expressed in Lactobacillus plantarum WCFS1. Results showed that the recombinant strain (W‐pMG36epuuE) exhibited better growth performance and survival rate compared to the control strain (W‐pMG36e) under ethanol‐stress conditions. In addition, it was found that the activities of superoxide dismutase and the concentration of glutathione of W‐pMG36epuuE were significantly higher than those of W‐pMG36e. This resulted in the decrease of intracellular reactive oxygen species (ROS) accumulation (10.34% lower than control). Moreover, heterologous expression of puuE in WCFS1 exhibited improved activities of two ATPases in membrane, increasing the cell membrane integrity (37.67% higher than control). These results revealed the role of the puuE gene in improving ethanol tolerance in O. oeni by decreasing ROS accumulation and enhancing cell membrane integrity.
Dark is durable, light is user‐friendly: The impact of color lightness on two product attribute judgments
Authors: Hagtvedt Henrik 
Published: September 2019

AbstractVisual design elements such as color do not only provide aesthetic appeal; they also convey information that consumers rely on when making unrelated product attribute judgments. Seven experiments reveal that consumers assess darker‐colored products to be more durable but less user‐friendly than lighter‐colored ones. Both outcomes are linked to the influence of color lightness on perceived weight, but the latter outcome appears to be more easily disrupted than the former one. Specifically, the impact of color lightness on user‐friendliness assessments, but not on durability assessments, is eliminated when the role of weight is unclear or when cognitive load is induced. However, consumers make greater downward adjustments in their durability assessments after physically handling a product with darker (vs. lighter) color. These findings highlight some of the potential complexities and nuances in consumer responses to color.
Long-term outcomes of open and endovascular treatment of recurrent carotid artery stenosis – a 16-year retrospective single centre case series
Authors: Ahmad Wael , Deeb Hiba , Otto Christoph , Kalmykov Egan L. , Barkans Arthurs , Kabbasch Christoph , Sharkawy Mohamed Ibrahim , Brunkwall Jan Sigge 
Published: September 2019

Summary: Background: The aim of this study is to evaluate perioperative as well as long-term outcomes in patients operated with carotid endarterectomy (CEA) or stenting (CAS) due to symptomatic or asymptomatic high-grade restenosis of the internal carotid artery (ICA). Patients and methods: In a retrospective analysis of our electronic database including 2980 patients who underwent carotid endarterectomy or stenting due to a symptomatic or asymptomatic high-grade stenosis of the ICA, between 2000 and 2016, we enrolled 111 patients with recurrent ICA stenosis. Results: An ipsilateral 2nd time restenosis (> 80 % in the asymptomatic and > 50 % in the symptomatic patients according to NASCET criteria) of ICA was detected in 13 patients (12 %); 3 of them were symptomatic. These patients were managed with either CEA (n = 5/38 %) or CAS (n = 8/62 %) with no perioperative stroke or death. The stroke-free survival rates at 2 and 8 years for CEA were 98 % and 98 % versus 100 % and 100 % for CAS respectively (P = .271). The type of the initial procedure (patch, CAS or interposition) did not play any significant role for the development of a 2nd time restenosis (P = .841). Conclusions: Redo-CEA/CAS seem to have similar results as primary procedures (as reported in the literature) with favorable periprocedural and long-term outcomes.
Conspecific and allospecific larval extracts entice mosquitoes to lay eggs and may be used in attract-and-kill control strategy
Authors: Faierstein Gabriel B. , Lu WeiYu , Sena Andréa K. L. S. , Barbosa Rosângela M. R. , Leal Walter S. 
Published: September 2019

AbstractOne of the strategies of integrated vector management is to lure gravid mosquitoes for surveillance purposes or to entice them to lay eggs in water containing toxins that kill the offspring (attract-and-kill or trap-and-kill). Typically, the major challenge of this approach is the development of a lure that stimulates oviposition plus a toxin with no deterrent effect. Bacillus thuringiensis var. israelensis (Bti) satisfies the latter criterion, but lures for these autocidal gravid traps are sorely needed. We observed that gravid Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus laid significantly more eggs in cups with extracts from 4th-stage larvae (4 L) of the same or different species. No activity was found when 4 L were extracted with hexane, diethyl ether, methanol, or butanol, but activity was observed with dimethyl sulfoxide extracts. Larval extracts contained both oviposition stimulant(s)/attractant(s) and deterrent(s), which partitioned in the water and hexane phases, respectively. Lyophilized larval extracts were active after a month, but activity was reduced by keeping the sample at 4 °C. In the tested range of 0.1 to 1 larvae-equivalent per milliliter, oviposition activity increased in a dose-dependent manner. In field experiments, Ae. aegpti laid significantly more eggs in traps loaded with larval extracts plus Bti than in control traps with water plus Bti.
Can an Imaging Marker of Consistency Predict Intraoperative Experience and Clinical Outcomes for Vestibular Schwannomas? A Retrospective Review
Authors: Macielak Robert J. , Harris Michael S. , Mattingly Jameson K. , Shah Varun S. , Prevedello Luciano M. , Adunka Oliver F. 
Published: September 2019

Abstract
Objective The main purpose of this article is to determine if vestibular schwannoma consistency as determined by tissue intensity on T2-weighted magnetic resonance imagings (MRIs) is predictive of intraoperative experience and postoperative clinical outcomes.
Study Design Retrospective chart review.
Setting Tertiary referral center.
Patients Seventy-seven patients diagnosed with vestibular schwannomas who were treated with microsurgical resection.
Intervention Diagnostic.
Main Outcome Measures Intraoperative measures include totality of resection, surgical time and cranial nerve VII stimulation and postoperative measures include House–Brackmann grade and perioperative complications.
Results Tumor consistency determined via tissue intensity on MRI was only found to correlate with surgical time, with a softer tumor being associated with a longer surgical time (p < 0.0001). However, this was primarily driven by tumor volume with larger tumors being associated with longer surgical time based on multivariate analysis. None of the other intraoperative or postoperative measures considered were found to correlate with tumor consistency.
Conclusions Tumor consistency determined by MRI is not predictive of intraoperative experience or postoperative outcomes in vestibular schwannomas. Tumor volume is the strongest driver of these outcome measures as opposed to tumor consistency.