bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of Human Immunodeficiency Virus Infection on Gut Microbiota α-Diversity: An Individual-level Meta-analysis
Authors: Tuddenham Susan A , Koay Wei Li A , Zhao Ni , White James R , Ghanem Khalil G , Sears Cynthia L ,   HIV Microbiome Re-analysis Consortium, Dillon Stephanie , Wilson Cara , Lozupone Catherine , Ward Honorine , Wanke Christine , Dubourg Gregory , Raoult Didier , Palmer Brent , Monaco Cynthia , Kwon Douglas , Mutlu Ece , Landay Alan , Paredes Roger , Noguera-Julian Marc , Sönnerborg Anders , Neogi Ujjwal , Nowak Rebecca , Ravel Jacques , Pérez-Santiago Josué , Smith Davey M , Pinto-Cardoso Sandra , Reyes-Terán Gustavo , Serrano-Villar Sergio , Gosalbes Soler Maria Jose , Vesterbacka Jan , Nowak Piotr , Pérez-Matute P , Oteo José A , D’Auria Giuseppe , Villar-García Judit , Yu Guoqin , Goedert James J 
Published: March 2019

AbstractBackgroundWhether human immunodeficiency virus (HIV) infection impacts gut microbial α-diversity is controversial. We reanalyzed raw 16S ribosomal RNA (rRNA) gene sequences and metadata from published studies to examine α-diversity measures between HIV-uninfected (HIV–) and HIV-infected (HIV+) individuals.MethodsWe conducted a systematic review and individual level meta-analysis by searching Embase, Medline, and Scopus for original research studies (inception to 31 December 2017). Included studies reported 16S rRNA gene sequences of fecal samples from HIV+ patients. Raw sequence reads and metadata were obtained from public databases or from study authors. Raw reads were processed through standardized pipelines with use of a high-resolution taxonomic classifier. The χ2 test, paired t tests, and generalized linear mixed models were used to relate α-diversity measures and clinical metadata.ResultsTwenty-two studies were identified with 17 datasets available for analysis, yielding 1032 samples (311 HIV–, 721 HIV+). HIV status was associated with a decrease in measures of α-diversity (P &lt; .001). However, in stratified analysis, HIV status was associated with decreased α-diversity only in women and in men who have sex with women (MSW) but not in men who have sex with men (MSM). In analyses limited to women and MSW, controlling for HIV status, women displayed increased α-diversity compared with MSW.ConclusionsOur study suggests that HIV status, sexual risk category, and gender impact gut microbial community α-diversity. Future studies should consider MSM status in gut microbiome analyses.
How Truly Conserved Is the “Well-Conserved” Vertebrate Stress Response?
Authors: Romero L M , Gormally B M G 
Published: March 2019

AbstractThe vertebrate stress response is considered to be a highly conserved suite of responses that are evolved to help animals survive noxious environmental stimuli. The two major pathways of the stress response include the catecholamine release that is part of the autonomic nervous system and comprises the immediate fight-or-flight response, and the slower release of corticosteroids from the hypothalamic–pituitary–adrenal axis that help orchestrate longer-term responses. These two pathways are present in every vertebrate yet examined, and the anatomical and physiological architecture underlying these pathways are consistent. Despite these structural similarities, however, recent data indicate substantial temporal and species variation in the actual regulation of these pathways. For example, activation of both pathways varies seasonally in some species but not others, and responses of both pathways can be extensively modulated by an individual’s previous experience. Consequently, even though the anatomy of the stress response is highly conserved, the activation and functional output is not highly conserved. Given this variation, it is perhaps not surprising that it is proving difficult to correlate individual stress responses with differences in fitness outcomes. This review summarizes the challenge of making broad generalized assumptions about fitness consequences of the stress response given the functional variation we observe.
A Highly Immunogenic, Protective, and Safe Adenovirus-Based Vaccine Expressing Middle East Respiratory Syndrome Coronavirus S1-CD40L Fusion Protein in a Transgenic Human Dipeptidyl Peptidase 4 Mouse Model
Authors: Hashem Anwar M , Algaissi Abdullah , Agrawal Anurodh Shankar , Al-amri Sawsan S , Alhabbab Rowa Y , Sohrab Sayed S , S. Almasoud Abdulrahman , Alharbi Naif Khalaf , Peng Bi-Hung , Russell Marsha , Li Xuguang , Tseng Chien-Te K 
Published: March 2019

AbstractBackgroundInfection control measures have played a major role in limiting human/camel-to-human transmission of Middle East respiratory syndrome coronavirus (MERS-CoV); however, development of effective and safe human or camel vaccines is warranted.MethodsWe extended and optimized our previous recombinant adenovirus 5 (rAd5)–based vaccine platform characterized by in vivo amplified and CD40-mediated specific responses to generate MERS-CoV S1 subunit-based vaccine. We generated rAd5 constructs expressing CD40-targeted S1 fusion protein (rAd5-S1/F/CD40L), untargeted S1 (rAd5-S1), and Green Fluorescent Protein (rAd5-GFP), and evaluated their efficacy and safety in human dipeptidyl peptidase 4 transgenic (hDPP4 Tg+) mice.ResultsImmunization of hDPP4 Tg+ mice with a single dose of rAd5-S1/F/CD40L elicited as robust and significant specific immunoglobulin G and neutralizing antibodies as those induced with 2 doses of rAd5-S1. After MERS-CoV challenge, both vaccines conferred complete protection against morbidity and mortality, as evidenced by significantly undetectable/reduced pulmonary viral loads compared to the control group. However, rAd5-S1– but not rAd5-S1/F/CD40L–immunized mice exhibited marked pulmonary perivascular hemorrhage post–MERS-CoV challenge despite the observed protection.ConclusionsIncorporation of CD40L into rAd5-based MERS-CoV S1 vaccine targeting molecule and molecular adjuvants not only enhances immunogenicity and efficacy but also prevents inadvertent pulmonary pathology after viral challenge, thereby offering a promising strategy to enhance safety and potency of vaccines.
Strong restrictions on the trait range of co-occurring species in the newly created riparian zone of the Three Gorges Reservoir Area, China
Authors: Zhang Aiying , Cornwell Will , Li Zhaojia , Xiong Gaoming , Fan Dayong , Xie Zongqiang 
Published: March 2019

AbstractAimsCommunity assembly links plant traits to particular environmental conditions. Numerous studies have adopted a trait-based approach to understand both community assembly processes and changes in plant functional traits along environmental gradients. In most cases these are long-established, natural or semi-natural environments. However, increasingly human activity has created, and continues to create, a range of new environmental conditions, and understanding community assembly in these ‘novel environments’ will be increasingly important.MethodsBuilt in 2006, the Three Gorges Dam, largest hydraulic project in China, created a new riparian area of 384 km2, with massively altered hydrology. This large, newly created ecosystem is an ideal platform for understanding community assembly in a novel environment. We sampled environment variables and plant communities within 103 plots located in both the reservoir riparian zone (RRZ) and adjacent non-flooded and semi-natural upland (Upland) at the Three Gorges Reservoir Area. We measured six traits from 168 plant species in order to calculate community-level distribution of trait values. We expected that the altered hydrology in RRZ would have a profound effect on the community assembly process for the local plants.Important FindingsConsistent with previous work on community assembly, the distribution of trait values (range, variance, kurtosis and the standard deviation of the distribution neighbor distances) within all plots was significantly lower than those from random distributions, indicating that both habitat filtering and limiting similarity simultaneously shaped the distributions of traits and the assembly of plant communities. Considering the newly created RRZ relative to nearby sites, community assembly was different in two main ways. First, there was a large shift in the mean trait values. Compared to Upland communities, plant communities in the RRZ had higher mean specific leaf area (SLA), higher nitrogen per unit leaf mass (Nmass), and lower maximum height (MH). Second, in the RRZ compared to the Upland, for the percentage of individual plots whose characteristic of trait values was lower than null distributions, the reductions in the community-level range for SLA, Nmass, nitrogen per unit leaf area (Narea) and phosphorus per unit leaf area (Parea) were much larger, suggesting that the habitat filter in this newly created riparian zone was much stronger compared to longer established semi-natural upland vegetation. This stronger filter, and the restriction to a subset of plants with very similar trait values, has implications for predicting riparian ecosystems’ responses to the hydrological alterations and further understanding for human’s effect on plant diversity and plant floras.
The Hebrew Translations of Euclid's Elements By Moses IBN Tibbon and by Jacob Ben Makhir–A Study of Differences in Book I
Authors: Elior Ofer 
Published: March 2019

Abstract
This study investigates differences between the Hebrew translation of Euclid's Elements made by Moses Ibn Tibbon and completed in 1270, and the Hebrew translation of the same work produced by Ibn Tibbon's nephew, Jacob ben Makhir, and completed in 1289. Focusing on Book I, the study shows that these differences extend to technical terms and make Ben Makhir's translation more accurate and more nuanced than Ibn Tibbon's. The greater accuracy of Ben Makhir's translation pertains also to his Hebrew formulations. Furthermore, apparent in his formulations is an attempt to make the Hebrew Elements more concise than the Arabic. On the basis of these findings, it is argued that Ben Makhir's translation is a reworking of Ibn Tibbon's, made with the aim of making the latter more succinct as well as more precise. A critical edition and an English translation of Ben Makhir's preface to his translation are presented in the Appendix.
The Israeli Defense Forces Point of Injury Antimicrobial Treatment Protocol – A New Protocol and Review of the Literature
Authors: Glick Yuval , Furer Ariel , Glick Karina , Yitzhak Avraham , Brosh Tal 
Published: March 2019

Abstract

Introduction
Combat wound infection is a common and serious complication, leading to significant morbidity and mortality. In 2005, a point of injury antimicrobial protocol was published by the Israel Defense Forces, in which Moxifloxacin was chosen. During 2016–2017, a revision of this protocol was performed and concluded with the publication of an updated protocol. The purpose of this report is to present this process and the revised protocol, together with a review of the literature.

Methods
We searched “Medline” and “Google Scholar” for studies dealing with antimicrobial prophylaxis in trauma, for militaries’ point of injury antimicrobial protocol protocols and for established surgical antimicrobial prophylaxis protocols.

Results
Point of injury antimicrobial protocol is aimed at preventing early infection and its complications. The choice of Moxifloxacin for this purpose may not be optimal since Moxifloxacin spectrum might be overly broad, there is scant evidence supporting it for this indication, and the available preparation does not meet distinctive technical requirements. Contrarily, Ceftriaxone seemed to have suitable microbiological, pharmacological and technical features.

Conclusion
Point of injury antimicrobial protocol should be used especially when evacuation and definitive surgical treatment are delayed. According to present scientific data and operational needs, Ceftriaxone was chosen for most penetrating injuries, with Metronidazole addition for penetrating abdominal and cranial trauma.
Army Body Composition Program Study Results Concerning: Enrollees Are More Over Fat Than Expected
Authors: Meyer Stephanie , Cole Renee 
Published: March 2019

Abstract

Introduction
Whether implementation of the Army Body Composition Program (ABCP) is meeting readiness objectives is unknown.

Objective
This study sought to primarily describe the extent of Active Duty Soldiers’ over-fatness when attending the initial ABCP nutrition class at an Army Nutrition clinic in Washington State; and secondarily to describe the proportion of these Soldiers meeting metabolic syndrome (MS) criteria.

Methods
Soldiers (189) in this cross-sectional study completed the following: a questionnaire developed for this study, anthropometric measurements, body fat assessment via AR 600–9 standards, and a laboratory blood draw for fasting glucose and lipid panel.

Results
Soldiers were predominantly male (76%), obese (BMI 32 kg/m2 for males and 30 kg/m2 for females), exceeded body fat standards by 3.8% for males and 7.3% for females, and 16% had three or more risk factors meeting MS diagnostic criteria. Waist circumference was the predominant MS risk factor for males and females.

Conclusion
Soldiers in this study had higher body fat percentages than expected with a majority of Soldiers classified as obese. Achieving and maintaining ABCP standards may be more challenging for obese Soldiers. To maintain Soldier readiness, commanders should consider intervening earlier when signs of weight gain are observed.
Percutaneous Peripheral Nerve Stimulation for Pain Reduction and Improvements in Functional Outcomes in Chronic Low Back Pain
Authors: Cohen Steven , Gilmore Christopher , Kapural Leonardo , Hanling Steven , Plunkett Anthony , McGee Meredith , Boggs Joseph 
Published: March 2019

Abstract
Chronic low back pain represents one of the most common sources of disability and a significant healthcare burden for the U.S. military. Present treatments for chronic back pain are often ineffective, poorly tolerated, invasive, destructive, and/or associated with complications and lead to the progression to invasive surgical procedures. There have been multiple calls for the development of a minimally invasive system that is effective without the risks or complications of existing surgical therapies, which could prevent the need for surgery and the recurrence of pain. The goal of this study was to evaluate a novel, minimally invasive approach using a percutaneous peripheral nerve stimulation (PNS) system designed to provide pain relief without surgery, to reduce complications, and provide a less-invasive treatment option. In nine subjects, percutaneous PNS improved participants’ function, as evidenced by clinically and statistically significant reductions in pain, disability, and pain interference. Subjects also experienced reductions in opioid and non-opioid analgesic medication usage and reported improvements in quality of life with treatment. There were no serious or unanticipated adverse events. These results demonstrate the potential of percutaneous PNS as a non-surgical therapy to treat chronic back pain without opioids.
Self-Reported Sleep, Anxiety, and Cognitive Performance in a Sample of U.S. Military Active Duty and Veterans
Authors: Rice Valerie J B , Schroeder Paul J 
Published: March 2019

AbstractUnhealthy sleep can interfere with U.S. military service members affective and cognitive functioning, and increase accident and injury risks. This study examined the relationship between U.S. active duty and veterans’ (n = 233) self-reported sleep (Pittsburgh Sleep Quality Index), anxiety (Zung Self-Rating Anxiety Scale), and cognitive performance (Automated Neuropsychological Assessment Metric). Statistical analyses included Pearson product moment correlations and multivariate analysis of variance, with Tukey-b post-hoc tests, with a p &lt; 0.05 significance level. Higher education, abstinence from sleep aids, longer time in active duty service, and being on active duty were correlated with better sleep and lower anxiety. Greater sleep disturbance, poor sleep quality, and sleepiness-related daytime dysfunction were associated with greater anxiety and slower response times, and lower response accuracy. Statistically controlling for anxiety diminished the magnitude and significance of the correlations between sleep and cognitive performance, suggesting that reducing anxiety will improve sleep and diminish cognitive performance effects. These findings suggest the need for addressing both sleep and anxiety for those with diagnosed sleep disorders, as well as using a procedural systems approach to decrease anxiety during missions that demand outstanding cognitive performance.
Pilot Study of a Novel Swine Model for Controlling Junctional Hemorrhage Using the iTClamp in Conjunction With Hemostatic Agents
Authors: Stuart Sean M , Zarow Gregory , Walchak Alexandra , McLean Julie , Roszko Paul 
Published: March 2019

AbstractExsanguinating hemorrhage is a primary cause of battlefield death. The iTClamp is a relatively new device (FDA approval in 2013) that takes a different approach to hemorrhage control by applying mechanism wound closure. However, no previous studies have explored the feasibility of utilizing the iTClamp in conjunction with hemostatic packing. To fill this important gap in the literature, a novel swine model was developed, and a total of 12 trials were performed using QuikClot Combat Gauze or XSTAT sponges in conjunction with the iTClamp to treat arterial injuries through 5 cm or 10 cm skin incisions in the groin, axilla, or neck. First-attempt application success rate, application time, and blood loss were recorded. Hemostasis was achieved on all wounds, though reapplication was required in one Combat Gauze and three XSTAT applications. Application averaged ~50% slower for Combat Gauze (M = 41 seconds, 95%CI: 22–32 seconds) than for XSTAT (M = 27 seconds, 95%CI: 35–47 seconds). XSTAT application was faster than Combat Gauze for each wound location and size. The 10 cm wounds took ~10 seconds (36%) longer to close (M = 27 seconds, 95%CI: 35–47 seconds) than the 5 cm wounds (M = 27 seconds, 95%CI: 35–47 seconds). Blood loss was similar for Combat Gauze (M = 51 mL, 95%CI: 25–76 mL) and XSTAT (M = 60 mL, 95%CI: 30–90 mL). Blood loss was roughly twice as great for 10 cm wounds (M = 73 mL, 95%CI: 47–100 mL) than for 5 cm wounds (M = 38 mL, 95%CI: 18–57 mL). This pilot study supports the feasibility of a novel model for testing the iTClamp in conjunction with hemostatic packing towards controlling junctional hemorrhage.
Mass Properties Comparison of Dismounted and Ground-Mounted Head-Supported Mass Configurations to Existing Performance and Acute Injury Risk Guidelines
Authors: Estep Patrick N , Bonts Emily G , Shivers Bethany L , Wurzbach John M , Novotny Brian L , Rybarczyk Kyle , Chancey Valeta Carol 
Published: March 2019

Abstract
In order to limit the aviator’s exposure to potentially unsafe helmet configurations, the U.S. Army Aeromedical Research Laboratory (USAARL) developed the USAARL Head-supported mass (HSM) Performance Curve and Acute Injury Risk Curve as guidelines for Army aviation HSM. These Curves remain the only established guidelines for Army HSM, but have limited applicability outside of the aviation environment. Helmet developers and program managers have requested guidelines be developed for the dismounted, ground-mounted, and airborne operating environments that consider currently fielded and proposed HSM configurations. The aim of this project was to measure mass properties (mass and center of mass offset) of currently fielded and proposed HSM configurations and compare them against the existing USAARL HSM Curve guidelines. Mass properties were collected for 71 unique dismounted and ground-mounted HSM configurations. None of the 71 HSM configurations met the Acute Injury Risk Curve recommendations, and only 11 of the 71 configurations met Performance Curve recommendations. While some helmets fell within acceptable limits, the addition of night vision goggles and protective masks pushed all configurations outside of the recommended guidelines. Future guidelines will need to be expanded to consider the operating environment, movement techniques, and primary mechanism of injury.
An Association Between Herpes Zoster Vaccination and Stroke Reduction Among Elderly Individuals
Authors: Klaric John S , Beltran Thomas A , McClenathan Bruce M 
Published: March 2019

AbstractHerpes zoster (HZ, shingles) affects individuals (60+ years) by reactivation of varicella virus from primary infection. Approximately one-third of the general population will develop HZ and are at increased risk of stroke. Our objective was describing possible associations between self-reported HZ vaccination and stroke with the Centers for Disease Control and Prevention’s Behavioral Risk Factors Surveillance System, a cross-sectional nationwide telephone survey. Non-institutionalized U.S. adults answered items concerning health risk behaviors. 2014 survey data were from 265,568 adults 50–79 years old. Multivariable Cox regressions adjusted for standard demographics, body mass index, and coronary heart disease showed that HZ-vaccinated individuals had lower risk of reporting stroke those not vaccinated (hazard ratio [HR] = 1.73). After stratification of participants into six 5-year age groups, adjusted weighted binary logistic regressions were conducted for each age group with stroke as outcome. The HZ-vaccinated group aged 65–69 years reported stroke approximately 50% less than those unvaccinated (adjusted Odds Ratio [aOR] = 1.51; 99% confidence interval [CI]:1.21,1.88). Secondary analyses indicated that this benefit was among HZ-vaccinated whites (aOR = 1.6, 95%CI:1.4,2.0), but not African Americans or Hispanics. These possible protective effects are not detected 10 years after recommended vaccine uptake. Limitations include not following participants longitudinally and that time between stroke and vaccination could not be determined.
Biomechanics of Blast TBI With Time-Resolved Consecutive Primary, Secondary, and Tertiary Loads
Authors: Przekwas Andrzej , Garimella Harsha T , Tan X Gary , Chen Z J , Miao Yuyang , Harrand Vincent , Kraft Reuben H , Gupta Raj K 
Published: March 2019

Abstract
Blast-induced traumatic brain injury (bTBI) has become a signature casualty of recent military operations. In spite of significant clinical and preclinical TBI research, current understanding of injury mechanisms and short- and long-term outcomes is limited. Mathematical models of bTBI biomechanics may help in better understanding of injury mechanisms and in the development of improved neuroprotective strategies. Until present, bTBI has been analyzed as a single event of a blast pressure wave propagating through the brain. In many bTBI events, the loads on the body and the head are spatially and temporarily distributed, involving the primary intracranial pressure wave, followed by the head rotation and then by head impact on the ground. In such cases, the brain microstructures may experience time/space distributed (consecutive) damage and recovery events. The paper presents a novel multiscale simulation framework that couples the body/brain scale biomechanics with micro-scale mechanobiology to study the effects of micro-damage to neuro-axonal structures. Our results show that the micro-mechanical responses of neuro-axonal structures occur sequentially in time with “damage” and “relaxation” periods in different parts of the brain. A new integrated computational framework is described coupling the brain-scale biomechanics with micro-mechanical damage to axonal and synaptic structures.