bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence for the Influence of Social Dominance Orientation and	Intergroup Relations on the Stigma of Schizophrenia
Authors: Lampropoulos Dimitrios , Troïan Jais , D’Amico Céline , Bentata Lise , Apostolidis Thémis 
Published: February 2019

Abstract. The stigma of schizophrenia is an intergroup phenomenon associated with issues of social power. We consider that the concept of stigma power should be extended to include intergroup relations that go beyond the aspect of the relation between “schizophrenic” and “normal.” With the present study, we intend to demonstrate that perceivers hold more stigmatizing attitudes toward a person with schizophrenia belonging to the outgroup and that especially when the perceiver has a higher Social Dominance Orientation (SDO). One hundred and sixty-one participants from the general population participated in our study (50.3% male, Mage = 39.14, SD = 16.36), which was based on a 2 (Sex of Target: male vs. female) × 2 (Sex of Participant: male vs. female) × SDO between-subjects design. The participants read a standardized vignette used in previous research, depicting a person presenting the symptoms of schizophrenia, and were then asked to complete questionnaires about SDO and intention to discriminate against the depicted individual. Our analysis showed the main effect of SDO on discriminating intentions. Simple slope analysis revealed that SDO predicts male participants’ discriminating intentions when the target is a woman, while this relation is not significant when the target is a man. The exact reverse pattern was observed among female participants. Our results suggest intergroup relations and ideological motivations underpin the stigma of schizophrenia. We conclude that different group belongings of individuals with schizophrenia as well as the public’s ideological motivations should be further considered by future research and anti-stigma efforts.
The Rupture Process of the 2018 Mw 6.9 Hawaiʻi Earthquake as Imaged by a Genetic Algorithm‐Based Back‐Projection Technique
Authors: Kehoe H. L. , Kiser E. D. , Okubo P. G. 
Published: February 2019

AbstractAn episode of unrest began at Kīlauea in April 2018 that produced both significant volcanic output and high rates of seismicity, including a Mw 6.9 earthquake on 4 May 2018. In this study, we image the rupture process of this earthquake using a genetic algorithm‐based back‐projection technique. The dominant feature of the earthquake is a slowly propagating western rupture, which shares similar characteristics with the region's largest recorded event in 1975 (Mw 7.7). The location of this western segment suggests that small asperities on this section of the décollement that frequently fail as slow slip events may achieve seismic slip rates when rupture is initiated on adjacent sections of the fault. Given the interaction between volcanic and seismic activity in this region, imaging the rupture properties of these events can improve our understanding of future geologic hazards in this region.
Satellite‐Based Detection of Daytime Supercooled Liquid‐Topped Mixed‐Phase Clouds Over the Southern Ocean Using the Advanced Himawari Imager
Authors: Noh Yoo‐Jeong , Miller Steven D. , Heidinger Andrew K. , Mace Gerald G. , Protat Alain , Alexander Simon P. 
Published: February 2019

AbstractInaccurate mixed‐phase cloud parameterizations over the Southern Ocean remain one of the largest sources of disagreement among global models in determining shortwave cloud radiative feedbacks. Suitable global observations supporting model improvements are currently unavailable. The conventional satellite cloud phase retrieval from passive radiometers is strongly biased toward cloud top without further information on the subcloud phase. Mixed‐phase clouds with the liquid‐top mixed‐phase (LTMP) structures are often classified simply as supercooled liquid. This paper presents a daytime multispectral detection algorithm for LTMP clouds, based on differential absorption between liquid and ice in shortwave infrared bands (1.61 and 2.25 μm). The LTMP algorithm, previously developed for polar‐orbiting sensors, is applied to Himawari‐8 Advanced Himawari Imager (the first of the next‐generation geostationary satellites) to probe subcloud phase for mixed‐phase clouds over the Southern Ocean. The results are compared with spaceborne active sensor data from CloudSat and CALIPSO. Ship‐based field experiment measurements are examined for selected cases to provide a more direct assessment of algorithm performance. The results show that applying the LTMP algorithm to geostationary satellites has potential to provide advanced time‐resolved observations for mixed‐phase clouds globally with improved sublayer cloud phase information that can support enhancement and validation of global models.
The lncRNA BDNF-AS is an epigenetic regulator in the human amygdala in early onset alcohol use disorders
Authors: Bohnsack John Peyton , Teppen Tara , Kyzar Evan J. , Dzitoyeva Svetlana , Pandey Subhash C. 
Published: February 2019

AbstractAdolescent alcohol drinking is known to contribute to the development and severity of alcohol use disorders (AUDs) later in adulthood. Recent studies have shown that long non-coding RNAs (lncRNAs) are critical for brain development and synaptic plasticity. One such lncRNA is natural occurring brain-derived neurotrophic factor antisense (BDNF-AS) that has been shown to regulate BDNF expression. The role ofBDNF-ASlncRNA in the molecular mechanisms of AUD is unknown. Here, we evaluated the expression and functional role ofBDNF-ASin postmortem amygdala of either early onset or late onset alcoholics (individuals who began drinking before or after 21 years of age, respectively) and age-matched control subjects.BDNF-ASexpression is increased in early onset but not in late onset AUD amygdala and appears to be regulated epitranscriptomically via decreasedN6-methyladenosine onBDNF-AS. Upregulation ofBDNF-ASis associated with a significant decrease in BDNF expression and increased recruitment of EZH2, which deposits repressive H3K27 trimethylation (H3K27me3) at regulatory regions in theBDNFgene in the early onset AUD group. Drinking during adolescence also contributed to significant decreases in activity-regulated cytoskeleton-associated protein (ARC) expression which also appeared to be mediated by increased EZH2 deposition of repressive H3K27me3 at theARCsynaptic activity response element. These results suggest an important role forBDNF-ASin the regulation of synaptic plasticity via epigenetic reprogramming in the amygdala of AUD subjects who began drinking during adolescence.
Energetics of the coupled electronic–structural transition in the rare-earth nickelates
Authors: Hampel Alexander , Liu Peitao , Franchini Cesare , Ederer Claude 
Published: February 2019

AbstractRare-earth nickelates exhibit a metal–insulator transition accompanied by a structural distortion that breaks the symmetry between formerly equivalent Ni sites. The quantitative theoretical description of this coupled electronic–structural instability is extremely challenging. Here, we address this issue by simultaneously taking into account both structural and electronic degrees of freedom using a charge self-consistent combination of density functional theory and dynamical mean-field theory, together with screened interaction parameters obtained from the constrained random phase approximation. Our total energy calculations show that the coupling to an electronic instability toward a charge disproportionated insulating state is crucial to stabilize the structural distortion, leading to a clear first order character of the coupled transition. The decreasing octahedral rotations across the series suppress this electronic instability and simultaneously increase the screening of the effective Coulomb interaction, thus weakening the correlation effects responsible for the metal–insulator transition. Our approach allows to obtain accurate values for the structural distortion and thus facilitates a comprehensive understanding, both qualitatively and quantitatively, of the complex interplay between structural properties and electronic correlation effects across the nickelate series.
miR-148a regulates expression of the transferrin receptor 1 in hepatocellular carcinoma
Authors: Babu Kamesh R. , Muckenthaler Martina U. 
Published: February 2019

AbstractTransferrin receptor 1 (TFR1) is a transmembrane glycoprotein that allows for transferrin-bound iron uptake in mammalian cells. It is overexpressed in various cancers to satisfy the high iron demand of fast proliferating cells. Here we show that in hepatocellular carcinoma (HCC) TFR1 expression is regulated by miR-148a. Within the TFR1 3′UTR we identified and experimentally validated two evolutionarily conserved miRNA response elements (MREs) for miR-148/152 family members, including miR-148a. Interestingly, analyses of RNA sequencing data from patients with liver hepatocellular carcinoma (LIHC) revealed a significant inverse correlation of TFR1 mRNA levels and miR-148a. In addition, TFR1 mRNA levels were significantly increased in the tumor compared to matched normal healthy tissue, while miR-148a levels are decreased. Functional analysis demonstrated post-transcriptional regulation of TFR1 by miR-148a in HCC cells as well as decreased HCC cell proliferation upon either miR-148a overexpression or TFR1 knockdown. We hypothesize that decreased expression of miR-148a in HCC may elevate transferrin-bound iron uptake, increasing cellular iron levels and cell proliferation.
Repellency Assessment of Nepeta cataria Essential Oils and Isolated Nepetalactones on Aedes aegypti
Authors: Reichert William , Ejercito Jadrian , Guda Tom , Dong Xujun , Wu Qingli , Ray Anandasankar , Simon James E. 
Published: February 2019

AbstractThere is an increased need for improved and affordable insect repellents to reduce transmission of rapidly spreading diseases with high mortality rates. Natural products are often used when DEET cannot be afforded or accessed and when consumers choose not to use a synthetic repellent. The essential oils from two newly bred Nepeta cataria (catnip) plants representing two different chemotypes and their respective isolated nepetalactone isomers were evaluated as mosquito repellents against Aedes aegypti mosquitoes that transmit the Zika and Dengue virus in a one choice landing rate inhibition assay. A dose response curve was generated for each treatment and a time course analysis of repellency was performed over 24 hours with a N. cataria essential oil sample. The results indicate that all essential oil samples and their respective purified nepetalactone isomers were able to achieve greater than 95% repellency. Between two and four hours, the ability to repel more than 95% of the mosquitoes diminished. At the lowest concentrations tested, the nepetalactones and crude essential oil samples were more effective than DEET at reducing the number of mosquito landings.
Aryl Azides as Phosphine-Activated Switches for Small Molecule Function
Authors: Lukasak Bradley , Morihiro Kunihiko , Deiters Alexander 
Published: February 2019

AbstractEngineered small molecule triggers are important tools for the control and investigation of biological processes, in particular protein function. Staudinger reductions of aryl azides to amines through the use of phosphines can trigger an elimination reaction, and thereby activation of a functional molecule, if an appropriately positioned leaving group is present. We conducted detailed investigations of the effect of aryl azide and phosphine structure on both the mechanism and kinetics of these reaction-induced eliminations and identified phosphine/azide pairs that enable complete activation within minutes under physiologically relevant conditions.
The bovine alveolar macrophage DNA methylome is resilient to infection with Mycobacterium bovis
Authors: O’Doherty Alan Mark , Rue-Albrecht Kevin Christophe , Magee David Andrew , Ahting Simone , Irwin Rachelle Elizabeth , Hall Thomas Jonathan , Browne John Arthur , Nalpas Nicolas Claude , Walsh Colum Patrick , Gordon Stephen Vincent , Wojewodzic Marcin Włodzimierz , MacHugh David Evan 
Published: February 2019

AbstractDNA methylation is pivotal in orchestrating gene expression patterns in various mammalian biological processes. Perturbation of the bovine alveolar macrophage (bAM) transcriptome, due toMycobacterium bovis(M. bovis) infection, has been well documented; however, the impact of this intracellular pathogen on the bAM epigenome has not been determined. Here, whole genome bisulfite sequencing (WGBS) was used to assess the effect ofM. bovisinfection on the bAM DNA methylome. The methylomes of bAM infected withM. boviswere compared to those of non-infected bAM 24 hours post-infection (hpi). No differences in DNA methylation (CpG or non-CpG) were observed. Analysis of DNA methylation at proximal promoter regions uncovered >250 genes harbouring intermediately methylated (IM) promoters (average methylation of 33–66%). Gene ontology analysis, focusing on genes with low, intermediate or highly methylated promoters, revealed that genes with IM promoters were enriched for immune-related GO categories; this enrichment was not observed for genes in the high or low methylation groups. Targeted analysis of genes in the IM category confirmed the WGBS observation. This study is the first in cattle examining genome-wide DNA methylation at single nucleotide resolution in an important bovine cellular host-pathogen interaction model, providing evidence for IM promoter methylation in bAM.
Convolutional neural networks can accurately distinguish four histologic growth patterns of lung adenocarcinoma in digital slides
Authors: Gertych Arkadiusz , Swiderska-Chadaj Zaneta , Ma Zhaoxuan , Ing Nathan , Markiewicz Tomasz , Cierniak Szczepan , Salemi Hootan , Guzman Samuel , Walts Ann E. , Knudsen Beatrice S. 
Published: February 2019

AbstractDuring the diagnostic workup of lung adenocarcinomas (LAC), pathologists evaluate distinct histological tumor growth patterns. The percentage of each pattern on multiple slides bears prognostic significance. To assist with the quantification of growth patterns, we constructed a pipeline equipped with a convolutional neural network (CNN) and soft-voting as the decision function to recognize solid, micropapillary, acinar, and cribriform growth patterns, and non-tumor areas. Slides of primary LAC were obtained from Cedars-Sinai Medical Center (CSMC), the Military Institute of Medicine in Warsaw and the TCGA portal. Several CNN models trained with 19,924 image tiles extracted from 78 slides (MIMW and CSMC) were evaluated on 128 test slides from the three sites by F1-score and accuracy using manual tumor annotations by pathologist. The best CNN yielded F1-scores of 0.91 (solid), 0.76 (micropapillary), 0.74 (acinar), 0.6 (cribriform), and 0.96 (non-tumor) respectively. The overall accuracy of distinguishing the five tissue classes was 89.24%. Slide-based accuracy in the CSMC set (88.5%) was significantly better (p < 2.3E-4) than the accuracy in the MIMW (84.2%) and TCGA (84%) sets due to superior slide quality. Our model can work side-by-side with a pathologist to accurately quantify the percentages of growth patterns in tumors with mixed LAC patterns.
Identification of reference genes for real-time PCR cytokine gene expression studies in sheep experimentally infected with Fasciola hepatica
Authors: Pacheco I. L. , Abril N. , Zafra R. , Morales-Prieto N. , Hernández V. Molina , Ruiz M. T. , Perez-Caballero R. , Martínez-Moreno A. , Pérez J. 
Published: February 2019

AbstractThe aim of this study was to validate reference genes for gene normalisation using qRT-PCR in hepatic lymph nodes (HLN) and livers from sheep infected with Fasciola hepatica during early and late stages of infection. To this end, a comprehensive statistical approach (RefFinder) encompassing four different methods of analysis (geNorm, BestKeeper, ΔCt method and NormFinder) was used to validate ten candidate reference genes. Stability analysis of gene expression followed by pairwise variation (Vn/Vn + 1) analysis revealed that PGK1, HSP90AA1 and GYPC were the most stable reference genes and suitable for qRT-PCR normalisation in both HLN and liver tissues. These three genes were validated against FoxP3, IL-10, TGF-β, TNF-α and IL-1β genes in the HLN tissue of sheep vaccinated with Cathepsin L1 from F. hepatica and unvaccinated infected and uninfected controls during early stages of infection. In the liver, the three reference genes were validated against TNF-α and IL-1β during chronic stages of infection with F. hepatica and in uninfected controls. Our study is the first to evaluate and validate sheep reference genes in order to provide tools for monitoring cytokines in Fasciola hepatica infected sheep target organs. Our results present an approach to elucidate the role of different cytokines in F. hepatica vaccinated and infected sheep.
Functional annotation of putative QTL associated with black tea quality and drought tolerance traits
Authors: Koech Robert. K. , Malebe Pelly M. , Nyarukowa Christopher , Mose Richard , Kamunya Samson M. , Joubert Fourie , Apostolides Zeno 
Published: February 2019

AbstractThe understanding of black tea quality and percent relative water content (%RWC) traits in tea (Camellia sinensis) by a quantitative trait loci (QTL) approach can be useful in elucidation and identification of candidate genes underlying the QTL which has remained to be difficult. The objective of the study was to identify putative QTL controlling black tea quality and percent relative water traits in two tea populations and their F1 progeny. A total of 1,421 DArTseq markers derived from the linkage map identified 53 DArTseq markers to be linked to black tea quality and %RWC. All 53 DArTseq markers with unique best hits were identified in the tea genome. A total of 5,592 unigenes were assigned gene ontology (GO) terms, 56% comprised biological processes, cellular component (29%) and molecular functions (15%), respectively. A total of 84 unigenes in 15 LGs were assigned to 25 different Kyoto Encyclopedia of Genes and Genomes (KEGG) database pathways based on categories of secondary metabolite biosynthesis. The three major enzymes identified were transferases (38.9%), hydrolases (29%) and oxidoreductases (18.3%). The putative candidate proteins identified were involved in flavonoid biosynthesis, alkaloid biosynthesis, ATPase family proteins related to abiotic/biotic stress response. The functional annotation of putative QTL identified in this current study will shed more light on the proteins associated with caffeine and catechins biosynthesis and % RWC. This study may help breeders in selection of parents with desirable DArTseq markers for development of new tea cultivars with desirable traits.
Quantitative analysis of self-organized patterns in ombrotrophic peatlands
Authors: Béguin Chloé , Brunetti Maura , Kasparian Jérôme 
Published: February 2019

AbstractWe numerically investigate a diffusion-reaction model of an ombrotrophic peatland implementing a Turing instability relying on nutrient accumulation. We propose a systematic and quantitative sorting of the vegetation patterns, based on the statistical analysis of the numbers and filling factor of clusters of both Sphagnum mosses and vascular plants. In particular, we define the transition from Sphagnum-percolating to vascular plant-percolating patterns as the nutrient availability is increased. Our pattern sorting allows us to characterize the peatland pattern stability under climate stress, including strong drought.
Toxic Injury to Muscle Tissue of Rats Following Acute Oximes Exposure
Authors: Jaćević Vesna , Nepovimova Eugenie , Kuča Kamil 
Published: February 2019

AbstractTherapeutic application of newly developed oximes is limited due to their adverse effects on different tissues. Within this article, it has been investigated which morphological changes could be observed in Wistar rats after the treatment with increasing doses of selected acetyl cholinesterase reactivators - asoxime, obidoxime, K027, K048, and K075. Subsequently, heart, diaphragm and musculus popliteus were obtained for pathohistological and semiquantitative analysis 24 hrs and 7 days after im administration of a single dose of 0.1 LD50, 0.5 LD50, and 1.0 LD50 of each oxime. Different muscle damage score was based on an estimation scale from 0 (no damage) to 5 (strong damage). In rats treated with 0.1 LD50 of each oxime, muscle fibres did not show any change. The intensive degeneration was found in all muscles after treatment with 0.5 LD50 of asoxime and obidoxime, respectively. Acute toxic muscle injury was developed within 7 days following treatment with 0.5 LD50 and 1.0 LD50 of each oxime, with the highest values in K048 and K075 group (P < 0.001 vs. control and asoxime), respectively. The early muscle alterations observed in our study seem to contribute to the pathogenesis of the oxime-induced toxic muscle injury, which probably manifests as necrosis and/or inflammation.
Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells
Authors: Gerlach Jan. P. , van Buggenum Jessie A. G. , Tanis Sabine E. J. , Hogeweg Mark , Heuts Branco M. H. , Muraro Mauro J. , Elze Lisa , Rivello Francesca , Rakszewska Agata , van Oudenaarden Alexander , Huck Wilhelm T. S. , Stunnenberg Hendrik G. , Mulder Klaas W. 
Published: February 2019

AbstractEnvironmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogeneous cellular responses to environmental signals at the mRNA and phospho-proteome level.
The cytochrome P450 CYP6DE1 catalyzes the conversion of α-pinene into the mountain pine beetle aggregation pheromone trans-verbenol
Authors: Chiu Christine C. , Keeling Christopher I. , Bohlmann Joerg 
Published: February 2019

AbstractThe recent outbreak of the mountain pine beetle (Dendroctonus ponderosae; MPB) has affected over 20 M hectares of pine forests in western North America. During the colonization of host trees, female MPB release the aggregation pheromone (−)-trans-verbenol. (−)-trans-Verbenol is thought to be produced from the pine defense compound (−)-α-pinene by cytochrome P450 (P450) dependent hydroxylation. MPB may also use P450s for the detoxification of other monoterpenes of the pine defense system. Here we describe the functional characterization of MPB CYP6DE1. CYP6DE1, but not the closely related CYP6DE2, used the bicyclic monoterpenes (−)-α-pinene, (+)-α-pinene, (−)-β-pinene, (+)-β-pinene and (+)-3-carene as substrates. CYP6DE1 was not active with other monoterpenes or diterpene resin acids that were tested as substrates. trans-Verbenol is the major product of CYP6DE1 activity with (−)-α-pinene or (+)-α-pinene as substrates. When tested with blends of different ratios of (−)-α-pinene and (+)-α-pinene, CYP6DE1 produced trans-verbenol with an enantiomeric profile that was similar to that produced by female MPB exposed to the α-pinene enantiomers.