bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coral Reef Calcification and Production After the 2016 Bleaching Event at Lizard Island, Great Barrier Reef
Authors: McMahon Ashly , Santos Isaac R. , Schulz Kai G. , Scott Anna , Silverman Jack , Davis Kay L. , Maher Damien T. 
Published: April 2019

AbstractSevere coral bleaching events have affected the GBR causing massive losses of hard coral cover. Here we use flow respirometry approaches to assess coral reef net ecosystem calcification (NEC) and net ecosystem production following the 2015/2016 bleaching event at Lizard Island in the northern Great Barrier Reef, a heavily impacted area. Previous studies conducted in 2008 and 2009 (Silverman et al., 2014, http://10.1016/j.gca.2014.09.011) were used as preimpact data. Lagrangian and Eulerian approaches provided varied results. Estimated NEC (29.1 to 137.7 mmol m−2 day−1) and NEP (−876.7 to 50.5 mmol m−2 day−1) rates in 2016 were highly sensitive to assumptions about reef water residence times and oceanic end‐member concentrations. Replicating the methodology used for the 2008 and 2009 study resulted in postbleaching NEC in 2016 of 32 ± 10.8 mmol m−2 day−1, 40%–46% lower than prebleaching estimates in 2008 (61 ± 12 mmol m−2 day−1) and 2009 (54 ± 13 mmol m−2 day−1). The slopes of the total alkalinity versus dissolved inorganic carbon plot decreased from ~ 0.3 in 2008 and 2009 to 0.1 in 2016, indicating elevated organic production and a shift in community function. Changes in NEC relative to the previous study were not driven by changing Ωarag. Coral cover shifted from 8.3% and 7.1% in 2008 and 2009 to 3.0% in 2016. We demonstrate a clear decrease in coral reef NEC following bleaching and highlight that subtle assumptions/methodological differences may create bias in the interpretation of results. Therefore, comparing coral reef metabolism data sets and predicting long‐term coral reef calcification based on existing short‐term data sets needs to be done with care.
Magnetospheric Signatures of STEVE: Implications for the Magnetospheric Energy Source and Interhemispheric Conjugacy
Authors: Nishimura Y. , Gallardo‐Lacourt B. , Zou Y. , Mishin E. , Knudsen D. J. , Donovan E. F. , Angelopoulos V. , Raybell R. 
Published: April 2019

AbstractWe present three STEVE (strong thermal emission velocity enhancement) events in conjunction with Time History of Events and Macroscale Interactions (THEMIS) in the magnetosphere and Defense Meteorological Satellite Program (DMSP) and Swarm in the ionosphere, for determining equatorial and interhemispheric signatures of the STEVE purple/mauve arc and picket fence. Both types of STEVE emissions are associated with subauroral ion drifts (SAID), electron heating, and plasma waves. The magnetosphere observations show structured electrons and flows and waves (likely kinetic Alfven, magnetosonic, or lower‐hybrid waves) just outside the plasmasphere. Interestingly, the event with the picket fence had a >~1 keV electron structure detached from the electron plasma sheet, upward field‐aligned currents (FACs), and ultraviolet emissions in the conjugate hemisphere, while the event with only the mauve arc did not have precipitation or ultraviolet emission. We suggest that the electron precipitation drives the picket fence, and heating drives the mauve as thermal emission.
Analytic Solutions for Tidal Propagation in Multilayer Coastal Aquifers
Authors: Bakker Mark 
Published: April 2019

AbstractA new analytic solution is presented for tidal propagation in multilayer coastal aquifers consisting of an arbitrary number of layers. The solution is derived using matrix calculus and may be applied to simulate tidal propagation in systems where aquifer layers are separated by leaky layers of low permeability or in stratified aquifers. The head, amplitude, and phase shift may be simulated for arbitrary tidal components. The transmissivity, storage coefficient, and loading efficiency may be different for every aquifer layer. Similarly, the resistance to vertical flow, storage coefficient, and loading efficiency may be different for every leaky layer. The aquifer system extends an infinite distance below the sea or may end abruptly at the shore line. In the former case, different aquifer and leaky layer properties may be specified below the sea and below the land. A homogeneous unconfined aquifer is simulated with 80 layers to demonstrate that the tidal signal propagates much farther inland in the deeper part than in the shallower part of an unconfined aquifer. This effect is enhanced significantly when layers of low permeability are present in the aquifer, even when these layers are fairly thin, such as clay lenses. The solution is implemented in Python and may be used, for example, to estimate aquifer parameters from head measurements in coastal aquifers.
Development and Application of a Functional Human Esophageal Mucosa Explant Platform to Eosinophilic Esophagitis
Authors: Kurten Richard C. , Rawson Renee , Shoda Tetsuo , Duong Loan D. , Adejumobi Dolapo , Levy Rebecca , Newbury Robert O. , Rothenberg Marc E. , Akuthota Praveen , Wright Benjamin L. , Dohil Ranjan , Jones Stacie M. , Aceves Seema S. 
Published: April 2019

AbstractThere is an increasing prevalence of esophageal diseases but intact human tissue platforms to study esophageal function, disease mechanisms, and the interactions between cell types in situ are lacking. To address this, we utilized full thickness human donor esophagi to create and validate the ex vivo function of mucosa and smooth muscle (n = 25). Explanted tissue was tested for contractile responses to carbachol and histamine. We then treated ex vivo human esophageal mucosa with a cytokine cocktail to closely mimic the Th2 and inflammatory milieu of eosinophilic esophagitis (EoE) and assessed alterations in smooth muscle and extracellular matrix function and stiffening. We found that full thickness human esophagus as well as the individual layers of circular and longitudinal muscularis propria developed tension in response to carbachol ex vivo and that mucosa demonstrated squamous cell differentiation. Treatment of mucosa with Th2 and fibrotic cytokines recapitulated the majority of the clinical Eosinophilic Esophagitis Diagnostic Profile (EDP) on fluidic transcriptional microarray. Transforming growth factor-beta-1 (TGFβ1) increased gene expression of fibronectin, smooth muscle actin, and phospholamban (p < 0.001). The EoE cocktail also increased stiffness and decreased mucosal compliance, akin to the functional alterations in EoE (p = 0.001). This work establishes a new, transcriptionally intact and physiologically functional human platform to model esophageal tissue responses in EoE.
Magnetic Resonance Imaging and Bioluminescence Imaging for Evaluating Tumor Burden in Orthotopic Colon Cancer
Authors: Ravoori M. K. , Margalit O. , Singh S. , Kim Sun-Hee , Wei W. , Menter D. G. , DuBois R. N. , Kundra V. 
Published: April 2019

AbstractQuantifying tumor burden is important for following the natural history of orthotopic colon cancer and therapeutic efficacy. Bioluminescence imaging (BLI) is commonly used for such assessment and has both advantages and limitations. We compared BLI and magnetic resonance imaging (MRI) for quantifying orthotopic tumors in a mouse model of colon cancer. Among sequences tested, T2-based MRI imaging ranked best overall for colon cancer border delineation, contrast, and conspicuity. Longitudinal MRI detected tumor outside the colon, indistinguished by BLI. Colon tumor weights calculated from MRI in vivo correlated highly with tumor weights measured ex vivo whereas the BLI signal intensities correlated relatively poorly and this difference in correlations was highly significant. This suggests that MRI may more accurately assess tumor burden in longitudinal monitoring of orthotopic colon cancer in this model as well as in other models.
Direct detection of bacteremia by exploiting host-pathogen interactions of lipoteichoic acid and lipopolysaccharide
Authors: Kubicek-Sutherland Jessica Z. , Vu Dung M. , Noormohamed Aneesa , Mendez Heather M. , Stromberg Loreen R. , Pedersen Christine A. , Hengartner Astrid C. , Klosterman Katja E. , Bridgewater Haley A. , Otieno Vincent , Cheng Qiuying , Anyona Samuel B. , Ouma Collins , Raballah Evans , Perkins Douglas J. , McMahon Benjamin H. , Mukundan Harshini 
Published: April 2019

AbstractBacteremia is a leading cause of death in sub-Saharan Africa where childhood mortality rates are the highest in the world. The early diagnosis of bacteremia and initiation of treatment saves lives, especially in high-disease burden areas. However, diagnosing bacteremia is challenging for clinicians, especially in children presenting with co-infections such as malaria and HIV. There is an urgent need for a rapid method for detecting bacteremia in pediatric patients with co-morbidities to inform treatment. In this manuscript, we have developed and clinically validated a novel method for the direct detection of amphiphilic pathogen biomarkers indicative of bacteremia, directly in aqueous blood, by mimicking innate immune recognition. Specifically, we have exploited the interaction of amphiphilic pathogen biomarkers such as lipopolysaccharides (LPS) from Gram-negative bacteria and lipoteichoic acids (LTA) from Gram-positive bacteria with host lipoprotein carriers in blood, in order to develop two tailored assays – lipoprotein capture and membrane insertion – for their direct detection. Our assays demonstrate a sensitivity of detection of 4 ng/mL for LPS and 2 ng/mL for LTA using a waveguide-based optical biosensor platform that was developed at LANL. In this manuscript, we also demonstrate the application of these methods for the detection of LPS in serum from pediatric patients with invasive Salmonella Typhimurium bacteremia (n = 7) and those with Staphylococcal bacteremia (n = 7) with 100% correlation with confirmatory culture. Taken together, these results demonstrate the significance of biochemistry in both our understanding of host-pathogen biology, and development of assay methodology, as well as demonstrate a potential new approach for the rapid, sensitive and accurate diagnosis of bacteremia at the point of need.
Host population density and presence of predators as key factors influencing the number of gregarious parasitoid Anaphes flavipes offspring
Authors: Samková Alena , Hadrava Jiří , Skuhrovec Jiří , Janšta Petr 
Published: April 2019

AbstractThe number of parasitoids developed per host is one of the major factors that influences future adult body size and reproductive success. Here, we examined four external factors (host species, heritability, host population density, and presence of predators) that can affect the number of the gregarious parasitoid Anaphes flavipes (Förster, 1841) (Hymenoptera: Mymaridae) wasps developing in one host. The effect of host population density on the number of parasitoid offspring developed per host was confirmed, and for the first time, we also showed that the number of offspring per host is influenced by the presence of predators. Low host density and presence of predators increases the number of wasps developed in one host egg. However, a higher number of A. flavipes in one host reduces A. flavipes body size and hence its future individual fertility and fitness. Our results highlighted the importance of some external factors that distinctly affect the number of wasp offspring. Therefore, in this context, we suggest that in comparison to solitary parasitoids, the gregarious parasitoid A. flavipes can better respond to various external factors and can more flexibly change its population density.
Placental secretion of apolipoprotein A1 and E: the anti-atherogenic impact of the placenta
Authors: Melhem Hassan , Kallol Sampada , Huang Xiao , Lüthi Michael , Ontsouka Corneille Edgar , Keogh Adrian , Stroka Deborah , Thormann Wolfgang , Schneider Henning , Albrecht Christiane 
Published: April 2019

AbstractHigh levels of atherogenic lipids in pregnancy are associated with health complications for the mother, the fetus and the newborn. As endocrine secretory tissue, the human placenta releases apolipoproteins (apos), particularly apoA1 and apoE. However, the magnitude and the directionality of the apo secretions remain unknown. We aimed to 1) determine the amount and orientation (apical-maternal versus basal-fetal) of placentally secreted apoA1 and apoE using human perfused placenta and primary trophoblast cell (PTC) culture, 2) compare apoA1 and apoE secretions of PTC with that of hepatocytes and 3) associate the obtained results with human blood levels by determining apoA1 and apoE concentrations in maternal and fetal serum samples. In perfused placenta and serum samples, apoA1 and apoE concentrations were significantly higher at the maternal compared to the fetal side. For apoE a similar trend was found in PTC. For apoA1, the secretion to the apical side declined over time while release to the basal side was stable resulting in significantly different apoA1 concentrations between both sides. Unexpectedly, PTC secreted significantly higher amounts of apoA1 and apoE compared to hepatocytes. Our data indicate that the placenta may play an important role in maternal and fetal cholesterol homeostasis via secretion of anti-atherogenic apos.
Limb linkage rehabilitation training-related changes in cortical activation and effective connectivity after stroke: A functional near-infrared spectroscopy study
Authors: Huo Congcong , Xu Gongcheng , Li Zengyong , Lv Zeping , Liu Qianying , Li Wenhao , Ma Hongzhuo , Wang Daifa , Fan Yubo 
Published: April 2019

AbstractStroke remains the leading cause of long-term disability worldwide. Rehabilitation training is essential for motor function recovery following stroke. Specifically, limb linkage rehabilitation training can stimulate motor function in the upper and lower limbs simultaneously. This study aimed to investigate limb linkage rehabilitation task-related changes in cortical activation and effective connectivity (EC) within a functional brain network after stroke by using functional near-infrared spectroscopy (fNIRS) imaging. Thirteen stroke patients with either left hemiparesis (L-H group, n = 6) and or right hemiparesis (R-H group, n = 7) and 16 healthy individuals (control group) participated in this study. A multichannel fNIRS system was used to measure changes in cerebral oxygenated hemoglobin (delta HbO2) and deoxygenated hemoglobin (delta HHb) in the bilateral prefrontal cortices (PFCs), motor cortices (MCs), and occipital lobes (OLs) during (1) the resting state and (2) a motor rehabilitation task with upper and lower limb linkage (first 10 min [task_S1], last 10 min [task_S2]). The frequency-specific EC among the brain regions was calculated based on coupling functions and dynamic Bayesian inference in frequency intervals: high-frequency I (0.6–2 Hz) and II (0.145–0.6 Hz), low-frequency III (0.052–0.145 Hz), and very-low-frequency IV (0.021–0.052 Hz). The results showed that the stroke patients exhibited an asymmetric (greater activation in the contralesional versus ipsilesional motor region) cortical activation pattern versus healthy controls. Compared with the healthy controls, the stroke patients showed significantly lower EC (p < 0.025) in intervals I and II in the resting and task states. The EC from the MC and OL to the right PFC in interval IV was significantly higher in the R-H group than in the control group during the resting and task states (p < 0.025). Furthermore, the L-H group showed significantly higher EC from the MC and OL to the left PFC in intervals III and IV during the task states compared with the control group (p < 0.025). The significantly increased influence of the MC and OL on the contralesional PFC in low- and very-low-frequency bands suggested that plastic reorganization of cognitive resources severed to compensate for impairment in stroke patients during the motor rehabilitation task. This study can serve as a basis for understanding task-related reorganization of functional brain networks and developing novel assessment techniques for stroke rehabilitation.
Genetic diversity and drug susceptibility patterns of the Mycobacterium tuberculosis complex in Yunnan, China
Authors: Bai Ruolan , Chi Shuijing , Li Xiaofei , Dai Xiting , Ji Zhenhua , Jian Miaomiao , Bi Yunfeng , Wang Feng , Ding Zhe , Luo Lisha , chen Taigui , Liu Aihua , Bao Fukai 
Published: April 2019

Abstract
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) which has been threatening global public health for many years. High genetic diversity is dominant feature of Mtb. Increasing cases of multidrug-resistant (MDR) tuberculosis (MDR-TB) is a serious public health problem to TB control in China. Spontaneous mutations in the Mtb genome can alter proteins which are the target of drugs, making the bacteria drug resistant. The purpose of the present study was to analyze the genotype of Mtb isolates from some areas in Yunnan, China and explore the association between genotypes and MDR-TB. Using spoligotyping, we identified Beijing genotypes, six non-Beijing genotypes and a number of orphan genotypes from 270 Mtb isolates from patients in Yunnan Province during 2014–2016. Of 270 Mtb isolates, 102 clinical Mtb strains were identified as drug-resistant (DR) by drug susceptibility testing (DST), among them, 52 MDR strains. Beijing genotypes occupied the highest MDR proportion (78.85%) followed by the orphan genotypes (15.38%). The characteristics of MDR strains showed high genetic diversity. The results will help to efficiently improve diagnosis and treatment and provide valuable information for Mtb molecular epidemiology.
The human duodenal mucosa harbors all components for a local renin angiotensin system
Authors: Spak Emma , Hallersund Peter , Edebo Anders , Casselbrant Anna , Fändriks Lars 
Published: April 2019

Abstract
The renin-angiotensin system (RAS) is present in the gastrointestinal (GI) tract but remains to be fully characterized, particularly in man. The duodenum plays a role in both the upper and lower GI regulation, as well as in distant organs. The present study investigates the presence and functional potential of RAS in the human duodenal mucosa of healthy individuals. Endoscopically acquired mucosal biopsies from healthy volunteers were examined using western blot, immunohistochemistry, and ELISA. Functionality was examined by using Ussing chambers and recording duodenal transmucosal potential difference (PD) and motility in vivo. Angiotensinogen, Angiotensin II (AngII) and its receptors (AT1R, AT2R) as well as to the RAS associated enzymes renin, ACE, and neprylisin were detected in all samples of duodenal mucosa. Migrating motility complex induced elevations of transmucosal PD were significantly larger after per-oral administration of the AT1R receptor antagonist candesartan. Fasting duodenal motility per se was not influenced by candesartan. The epithelial current produced by duodenal mucosae mounted in Ussing chambers increased significantly after addition of AngII to specimens where the AT1R was blocked using losartan. The epithelial current also increased after addition of the AT2R-selective agonist C21. Immunostaining and pharmacological data demonstrate the presence of a local RAS in the human duodenal mucosa with capacity to influence epithelial ion transport by way of particulary the AT2R.
Evaluation of an Explicit Instructional Approach to Teach Grammatical Forms to Children With Low-Symptom Severity Autism Spectrum Disorder
Authors: Bangert Katherine J. , Halverson Danneka M. , Finestack Lizbeth H. 
Published: April 2019

Purpose
Weaknesses in the use of grammatical forms may reduce the functional use of language for verbally expressive children with autism spectrum disorder (ASD) and exacerbate difficulties with academic and social skill development. This early efficacy study evaluated a combined explicit–implicit instructional approach to teach novel grammatical forms to children with ASD.

Method
Seventeen children with ASD between the ages of 4 and 10 years who demonstrated weaknesses in expressive grammatical language completed 2 tasks, each targeting a different novel grammatical form. One form was a gender marking, which required the child to modify the verb if the sentence subject was a boy. The other form was a person marking, which required the child to modify the verb if the sentence subject was the 1st person, “I.” Each form was targeted using implicit-only instruction or combined explicit–implicit instruction. With implicit-only instruction, the examiner presented models and recasts of the targeted form. With explicit–implicit instruction, the examiner presented the rule guiding the form as well as models and recasts. Learning was assessed during each of 4 treatment sessions and after a 1-week delay in 2 contexts.

Results

For the gender target form, significantly more children reliably produced the target form with explicit–implicit instruction (χ
2
= 4.10,
p =
.04). For the person target form, the difference in instruction was not statistically significant. Task performance revealed a positive association with receptive language skills, but not age, nonverbal intelligence, or severity of autism-related behaviors for the person form.

Conclusion
This study provides preliminary evidence that expressively verbal children with low-symptom severity ASD can successfully learn novel grammatical forms with intervention that comprises both explicit and implicit instruction.
Six‐phase dual‐interleaved buck‐boost converter for high power‐density automotive applications
Authors: Velázquez‐Elizondo P. E. , Araujo‐Vargas I. , Villarruel‐Parra A. , Gómez‐Olguín F. J. , Cano‐ Pulido K. , Granados‐Luna T. R. 
Published: April 2019

This study presents a 30 kW, six‐phase dual‐interleaved buck‐boost converter which uses an interphase transformer together with six buck‐boost switching arms hard switched at 75 kHz for electric/hybrid vehicle applications. Parameters and construction details of the interphase transformer and common inductors are provided together with a description of the selection of semiconductor devices. Weight, power losses, and temperature rise are evaluated at rated power conditions showing experimental results with a 7 kW/kg gravimetric power density achieved with the converter prototype.
Hepatitis C – von der individuellen Heilung zur weltweiten Elimination?
Authors: Boettler Tobias , Lohmann Volker , Bartenschlager Ralf 
Published: April 2019

AbstractWith the implementation of highly effective direct acting antivirals (DAAs), global control or even elimination of chronic hepatitis C virus (HCV) infection might have come into reach. In fact, DAA therapy leads to complete virus elimination, defined as sustained viral response (SVR), in the vast majority of patients. Moreover, in patients without cirrhosis, the risk of developing HCC after DAA therapy is significantly reduced. For viremic patients who have already received DAA therapy, a distinction must be made between relapse and reinfection. The rate of new infections remains high and many infected individuals are undiagnosed. In order to come closer to the WHO goal of eliminating HCV worldwide by 2030, programs are needed to identify and treat all HCV-infected individuals. Strategies are missing in most countries to achieve this goal. Generic DAA therapies are available in some countries and appear to have similar cure rates compared to those obtained with the original drugs. The high variability of HCV, the numerous strategies of the virus to escape the immune response, and the lack of a suitable small animal model are key hurdles for vaccine development. Currently, the efficacy of two vaccine candidates is being investigated in clinical trials. The development of a protective vaccine is important, despite available therapy, to sustainably reduce the rate of new infections both in developing countries and in people with risk behavior.