bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple cross displacement amplification-based lateral flow biosensor for rapid and sensitive detection of Helicobacter pylori
Authors: Chen Yanfei , Zhou Juan , Wang Jiao , He Xi , Huang Xiaolan , Xiao Fei , Jia Nan , Wang Yi , Zhong Xuemei 
Published: November 2024

Helicobacter pylori (H. pylori, HP), recognized globally as one of the most widespread bacteria, serves as primary etiological agent for numerous gastroduodenal diseases, highlighting the urgent need to develop rapid and sensitive diagnostic method for H. pylori infection. Here, we devised a new diagnostic test that merged multiple cross displacement amplification (MCDA) with nanoparticle‐based lateral flow biosensor (LFB), termed HP‐MCDA‐LFB, to facilitate the rapid and sensitive detection of H. pylori. The whole detection workflow, which includes stages such as DNA template extraction (~15 min), MCDA pre-amplification (~40 min), and result readout (~2 min), was efficiently completed within 1 h. After optimization, the HP-MCDA-LFB assay demonstrated remarkable sensitivity in detecting H. pylori, with a detection threshold as low as 60 fg of genomic DNA (~56 copies) per microliter. Furthermore, the HP-MCDA-LFB assay also achieved a perfect specificity rate of 100%, exhibiting no cross-reactivity with non-Helicobacter isolates. Particularly, the clinical feasibility of HP-MCDA-LFB assay was validated using 40 antral mucosa samples, among which 17 tested positive for H. pylori, which was in complete agreement with the results obtained from the rapid urease test. In conclusion, the HP‐MCDA‐LFB method developed in this study is a rapid, sensitive, and specific method for diagnosing H. pylori infection, indicating great potential for H. pylori eradication therapy.
Clinical value of heparin-binding protein in adult bacterial intracranial infection
Authors: Guan Linsai , Wang Feiyao , Chen Jingni , Xu Yanxin , Zhang Weixing , Zhu Jianping 
Published: November 2024

BackgroundThe accurate and sensitive diagnosis of intracranial infection continues to pose a critical challenge. This study aimed to probe into the clinical value of heparin binding protein (HBP) in bacterial intracranial infection.MethodsPatients suspected of having bacterial intracranial infection and admitted to Shanghai General Hospital from November 2021 to November 2023 were selected as study subjects and divided into an infected group and a non-infected group. The receiver operating characteristic (ROC) curve was constructed to compare the diagnostic accuracy of HBP, procalcitonin (PCT), and C-reactive protein (CRP), as well as their value in differentiating Gram-positive bacteria and Gram-negative bacterial infections.ResultsAccording to the results of bacterial identification, the infected groups were divided into a Gram-negative bacteria group (n = 142) and a Gram-positive bacteria group (n = 128), while the non-infected group comprised 120 patients after neurosurgery involving dura opening. Statistically significant differences were observed in the levels of HBP, PCT, and CRP between the infected group and the non-infected group (all p&lt; 0.05). Receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) of HBP was 0.935, and the AUCs of PCT and CRP were 0.931 and 0.863, respectively. In the comparison of HBP, PCT, and CRP levels in the Gram-negative bacteria and Gram-positive bacteria groups, the AUCs were 0.816, 0.602, and 0.591, respectively. When the cutoff value of HBP was 72.34 ng/mL, its specificity reached 96.1% and its sensitivity was 57.8%. When PCT and CRP levels were less than 1.67 ng/mL and 23.12 ng/mL, respectively, both the sensitivity (52.3%, 53.1%) and specificity (66.9%, 59.9%) were relatively low.ConclusionHBP, PCT, and CRP can be employed as diagnostic indicators for bacterial intracranial infection. HBP (&gt;72.34 ng/mL) can act as an important index for the diagnosis of Gram-negative bacteria in patients with intracranial infection.
Deciphering the pathogen heterogeneity for precise diagnosis and personalized therapeutics of infections after kidney transplantation: insights from metagenomic next-generation sequencing
Authors: Ye Xin , Lin Yuxin , Yang Jiangnan , Qi Baocui , Wei Xuedong , Huang Yuhua , Wang Liangliang 
Published: November 2024

IntroductionThe aim of this study was to compare the detection performance of mNGS against that of conventional tests (CT) in patients suffering from infection after kidney transplantation.MethodsA total of 138 samples from 85 kidney transplant patients with acute or chronic infections were simultaneously analyzed using mNGS and CT from July 2021 to August 2023.ResultsCompared with CT, mNGS demonstrated a higher sensitivity (95.96% vs. 27.27%) but lower specificity (48.72% vs. 84.62%) in pathogen detection. Moreover, mNGS exhibited significant advantages in detecting mixed and rare infections. The pathogens commonly identified in kidney transplant patients were severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), followed by Pneumocystis jirovecii and Cytomegalovirus (CMV). mNGS guided the precise clinical diagnosis in 89.13% of cases and assisted in altering therapeutics from empirical antibiotic approaches to personalized plans in 56.10% of cases, including treatment escalation (40.65%), initiation (11.38%), drug adjustment (3.25%), and de-escalation (0.81%).DiscussionOur study demonstrated the superior detection performance of mNGS and its significant clinical value. This reflected the great potential of mNGS as a complementary clinical detection technology for kidney transplant patients.
Transcultural validation of the basic empathy scale for children in Arabic language: psychometric properties among schoolchildren in Tunisia
Authors: Chokri Imed , Peyrotte Marianne , Zanna Omar , Jarraya Mohamed 
Published: November 2024

IntroductionTo date, there is no empathy test specifically designed in the Arabic language for children. When administered in the Arab world, these tests are often provided in French or English, known to induce numerous intercultural biases.AimThe objective of our study is to, for the first time, propose a translation and validation of the Basic Empathy Scale for Children in the Arabic language.MethodFive hundred and thirty three Tunisian schoolchildren (age = 10.15 ± 1.39 years, 55.2% girls and 44.8% boys) participated voluntarily in the study. The students answered tow scales that measure the Empathy and the social desirability.ResultThe Arabic version of the BES-C demonstrated a 3-factor structure: cognitive empathy, emotional contagion, and emotional disconnection previously observed in other cultural contexts. McDonald’s omega is 0.72 for the Arabic version of the BES-C. The test–retest intra-class correlation coefficient over six weeks (n = 180) was 0.67. The findings show an increase in cognitive empathy with age, stability in emotional contagion, and a gradual decrease in emotional disconnection. Girls scored higher in emotional contagion, while boys showed a slight predominance of emotional disconnection. No significant gender differences were found in cognitive empathy.ConclusionThe Arabic version of the BES-C is a dependable tool for evaluating empathy in Arabic-speaking children, paving the way for its use in cross-cultural research and specialized interventions.
Exposure to brominated flame retardants in utero and through lactation delays the development of DMBA-induced mammary cancer: potential effects on subtypes?
Authors: Juarez Melany N. , McDermott Alec , Wade Michael G. , Plante Isabelle 
Published: November 2024

IntroductionBrominated flame retardants (BFRs) are chemical compounds used to reduce the flammability of various products; some BFRs exhibit endocrine-disrupting properties and can leach into the environment leading to human and wildlife exposure. The mammary gland has specific vulnerability windows during which it is more sensitive to the effects of endocrine disrupting compounds (EDCs), such as the in utero life, puberty and pregnancy. Our previous studies revealed precocious mammary gland development, disruptions in junctional proteins, and altered proliferation-apoptosis balance during puberty in rats exposed to BFRs in utero and through lactation. Such effects have been associated with increased mammary cancer risk.ObjectiveThe current study aimed to determine if in utero and lactational exposure to BFRs renders the mammary gland more susceptible to 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary cancer.MethodsDams were exposed to a BFRs mixture (0. 0.06 or 60 mg/kg/day), and mammary cancer was induced in pups using DMBA at post-natal day 46. Tumors onset and growth were monitored, and tumors were characterized using histology and molecular biology.ResultsAlthough BFRs exposure did not significantly affect mammary tumor number or burden, it showed significant delay in mammary tumor onset and growth in BFR-exposed animal. These effects could potentially be due to BFRs’ impact on cellular responses, DMBA metabolism, or mammary gland shift of the sensitivity window. Molecular analysis of mammary tumors showed a shift in the ratio of luminal A, luminal B, and (HER2)-enriched tumors, and an increase in triple-negative breast cancer (TNBC) subtypes in BFR-exposed animals. Additionally, BFRs exposure showed lung lesions indicative of inflammation, independent of mammary cancer development.ConclusionOur study highlights the complex relationship between BFRs exposure and mammary cancer risk, emphasizing the need for further investigation into underlying mechanisms and long-term effects of BFRs on mammary gland development and carcinogenesis.
Spatiotemporal pattern of water hyacinth (Pontederia crassipes) distribution in Lake Tana, Ethiopia, using a random forest machine learning model
Authors: Belayhun Matiwos , Chere Zerihun , Abay Nigus Gebremedhn , Nicola Yonas , Asmamaw Abay 
Published: November 2024

Water hyacinth (Pontederia crassipes) is an invasive weed that covers a significant portion of Lake Tana. The infestation has an impact on the lake’s ecological and socioeconomic systems. Early detection of the spread of water hyacinth using geospatial techniques is crucial for its effective management and control. The main objective of this study was to examine the spatiotemporal distribution of water hyacinth from 2016 to 2022 using a random forest machine learning model. The study used 16 variables obtained from Sentinel-2A, Sentinel-1 SAR, and SRTM DEM, and a random forest supervised classification model was applied. Seven spectral indices, five spectral bands, two Sentinel-1 SAR bands, and two topographic variables were used in combination to model the spatial distribution of water hyacinth. The model was evaluated using the overall accuracy and kappa coefficient. The findings demonstrated that the overall accuracy ranged from 0.91 to 0.94 and kappa coefficient from 0.88 to 0.92 in the wet season and 0.93 to 0.95 and 0.90 to 0.93 in the dry season, respectively. B11 and B5 (2022), VH, soil adjusted vegetation index (SAVI), and normalized difference water index (NDWI) (2020), B5 and B12 (2018), and VH and slope (2016) are the highly important variables in the classification. The study found that the spatial coverage of water hyacinth was 686.5 and 650.4 ha (2016), 1,851 and 1,259 ha (2018), 1,396.7 and 1,305.7 ha (2020), and 1,436.5 and 1,216.5 ha (2022) in the wet and dry seasons, respectively. The research findings indicate that variables derived from optical (Sentinel-2A and SRTM) and non-optical (Sentinel-1 SAR) satellite imagery effectively identify water hyacinth and display its spatiotemporal spread using the random forest machine learning algorithm.
Unbiased immunome characterisation correlates with COVID-19 mRNA vaccine failure in immunocompromised adults
Authors: H-Vázquez Juan , Cal-Sabater Paloma , Arribas-Rodríguez Elisa , Fiz-López Aida , Perez-Segurado Candido , Martín-Muñoz Álvaro , De Prado Ángel , Perez Mazzali Marina , de Castro Carolina G. , del Hierro Alejandro G. , de la Fuente Graciani Ignacio , Pérez González Sonia , Gutiérrez Sara , Tellería Pablo , Novoa Cristina , Rojo Rello Silvia , Garcia-Blesa Antonio , Sedano Rosa , Martínez García Ana María , Garcinuño Pérez Sonsoles , Domínguez-Gil Marta , Hernán García Cristina , Guerra Ma Mercedes , Muñoz-Sánchez Eduardo , Barragan-Pérez Cristina , Diez Morales Soraya , Casazza Donnarumma Oriana , Ramos Pollo Daniel , Santamarta Solla Natalia , Álvarez Manzanares Paula Ma , Bravo Sara , García Alonso Cristina , Avendaño Fernández Luis Alberto , Gay Alonso Jenifer , Garrote José A. , Arranz Eduardo , Eiros José María , Rescalvo Santiago Fernando , Quevedo Villegas Carolina , Tamayo Eduardo , Orduña Antonio , Dueñas Carlos , Peñarrubia María Jesús , Cuesta-Sancho Sara , Montoya María , Bernardo David 
Published: November 2024

IntroductionCoronavirus disease 2019 (COVID-19) affects the population unequally, with a greater impact on older and immunosuppressed people.MethodsHence, we performed a prospective experimental cohort study to characterise the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination in immune-compromised patients (older adults and oncohaematologic patients), compared with healthy counterparts, based on deep characterisation of the circulating immune cell subsets.Results and discussionWhile acquired humoral and cellular memory did not predict subsequent infection 18 months after full vaccination, spectral and computational cytometry revealed several subsets within the CD8+ T-cells, B-cells, natural killer (NK) cells, monocytes and TEMRA Tγδ cells that were differentially expressed in individuals who were subsequently infected and not infected not just following immunisation, but also prior to vaccination. Of note, we found up to seven clusters within the TEMRA Tγδ cell population, with some of them being expanded and others decreased in subsequently infected individuals. Moreover, some of these cellular clusters were also related to COVID-19-induced hospitalisation in oncohaematologic patients. Therefore, we have identified a cellular signature that even before vaccination is related to COVID-19 vulnerability as opposed to the acquisition of cellular and/or humoral memory following vaccination with SARS-CoV-2 messenger RNA (mRNA) vaccines.
Molecular genetics in adult-onset Still’s disease: next-generation sequencing in 24 patients and literature review
Authors: Prieto-Peña Diana , Labrador-Sánchez Eztizen , Melero-González Rafael B. , Antón-Pagés Fred , Palmou-Fontana Natalia , Alvarez-Reguera Carmen , Paz-Gandiaga Nerea , Blanco Ricardo 
Published: November 2024

ObjectiveNext-generation sequencing (NGS) panels are increasingly used for the diagnosis of monogenic systemic autoinflammatory diseases (SAIDs). However, their role in patients with adult-onset Still’s disease (AOSD) remains unknown. This study aims to assess the usefulness of NGS panels in AOSD patients to improve diagnosis and management of the disease.MethodsThis observational, multicenter study included all patients with AOSD diagnosis who underwent NGS panel testing in northern Spain. Clinical manifestations, laboratory parameters, complications, and therapeutic responses were recorded.ResultsA total of 24 patients (16 men, 8 women) with an average age of 42.2 ± 17.9 (mean ± SD) years, in whom NGS was performed, fulfilled the Yamaguchi and/or Fautrel criteria for AOSD. The most common symptoms, apart from fever, were skin rash (75%), asthenia (91.7%), and articular manifestations (91.7%). All patients had elevated acute-phase reactant levels and hyperferritinemia. Almost all patients received oral glucocorticoids as initial therapy. Conventional disease-modifying antirheumatic drugs (cDMARDs) were used in 17 (70.8%) patients and biologic therapy in 13 (54.1%) patients. Genetic variants were observed in 5 (20.8%) patients. None of them were classified as pathogenic. Variants of uncertain significance (VUS) were identified in NOD2 (c.2104C&gt;T and c.2251G&gt;A), TNFRSF1A (c.224C&gt;T), TNFAIP3 (c.1939A&gt;C), and SCN9A (c.2617G&gt;A). Atypical manifestations and/or therapeutic refractoriness were observed in patients carrying genetic variants, except for one patient with the TNFAIP3 VUS. Four out of five patients with VUS had a severe and refractory course of the disease and required biologic therapy.ConclusionNGS was useful to rule out the presence of pathogenic genetic variants related to other SAIDs and to detect VUS that may help identify patients at risk for atypical and severe manifestations and poor response to conventional therapy.
Climatic variables and their relationship with vector-borne disease cases in Colombia, 2011-2021
Authors: Gastelbondo-Pastrana Bertha , Echeverri-De la Hoz Daniel , Sanchez Liliana , García Yuranis , Espitia-Delgado Yeiner , Lopez Yalile , Yasnot-Acosta Maria F. , Arrieta German , Mattar Salim 
Published: November 2024

IntroductionVector-borne diseases are a public health problem in tropical countries, especially Malaria, Leishmaniasis, Chagas, and the arboviral diseases Dengue, Chikungunya, and Zika. Tropical diseases represent a significant challenge to health systems in developing countries.ObjectiveThis study aimed to explore the association between climatic variables and primary vector-borne diseases in Colombia between 2011-2021.MethodsA retrospective analysis of the epidemiological data of Malaria, Leishmania, Chagas, Dengue, Chikungunya, and Zika and the climatic data recorded in 2011-2021 was carried out. The cases were obtained from SIVIGILA of the Colombian Ministry of Health. The climatic data were obtained from WorldClim.ResultsLeishmania, Malaria, and Chagas occur in the departments of Caquetá (located in the Amazon rainforest), Chocó (known for its high rainfall), and Casanare (a region with a high prevalence of Chagas disease). There was no statistical correlation between the number of cases and climatic variables, except for malaria cases in which precipitation had a Pearson correlation value of 0.415. The results predicted an increase of 1.469 malaria cases for every unit increase in precipitation. The cases of dengue, Zika, and chikungunya had similar behavior, with the majority occurring in the departments of Valle del Cauca, Antioquia, Norte de Santander, and Bolívar.ConclusionsExcept for Malaria, the distribution of cases did not directly correlate with temperature and precipitation. Temperature and precipitation did not explain the epidemiological profile of the studied diseases.
Bibliometric and visual analysis of radiomics for evaluating lymph node status in oncology
Authors: Lyu Gui-Wen , Tong Tong , Yang Gen-Dong , Zhao Jing , Xu Zi-Fan , Zheng Na , Zhang Zhi-Fang 
Published: November 2024

BackgroundRadiomics, which involves the conversion of digital images into high-dimensional data, has been used in oncological studies since 2012. We analyzed the publications that had been conducted on this subject using bibliometric and visual methods to expound the hotpots and future trends regarding radiomics in evaluating lymph node status in oncology.MethodsDocuments published between 2012 and 2023, updated to August 1, 2024, were searched using the Scopus database. VOSviewer, R Package, and Microsoft Excel were used for visualization.ResultsA total of 898 original articles and reviews written in English and be related to radiomics for evaluating lymph node status in oncology, published between 2015 and 2023, were retrieved. A significant increase in the number of publications was observed, with an annual growth rate of 100.77%. The publications predominantly originated from three countries, with China leading in the number of publications and citations. Fudan University was the most contributing affiliation, followed by Sun Yat-sen University and Southern Medical University, all of which were from China. Tian J. from the Chinese Academy of Sciences contributed the most within 5885 authors. In addition, Frontiers in Oncology had the most publications and transcended other journals in recent 4 years. Moreover, the keywords co-occurrence suggested that the interplay of “radiomics” and “lymph node metastasis,” as well as “major clinical study” were the predominant topics, furthermore, the focused topics shifted from revealing the diagnosis of cancers to exploring the deep learning-based prediction of lymph node metastasis, suggesting the combination of artificial intelligence research would develop in the future.ConclusionThe present bibliometric and visual analysis described an approximately continuous trend of increasing publications related to radiomics in evaluating lymph node status in oncology and revealed that it could serve as an efficient tool for personalized diagnosis and treatment guidance in clinical patients, and combined artificial intelligence should be further considered in the future.
Disorders of acid-base balance promote rumen lipopolysaccharide biosynthesis in dairy cows by modulating the microbiome
Authors: Hou Guobin , You Jingtao , Zhuang Yimin , Gao Duo , Xu Yiming , Jiang Wen , Li Sumin , Zhao Xinjie , Chen Tianyu , Zhang Siyuan , Liu Shuai , Wang Wei , Li Shengli , Cao Zhijun 
Published: November 2024

IntroductionDisorders of acid-base balance in the rumen of dairy cows have a significant impact on their health and performance. However, the effect of transient differences in pH on susceptibility to subacute ruminal acidosis (SARA) and lipopolysaccharide (LPS) biosynthesis in dairy cows remains unclear.MethodsIn this study, milk, serum, and rumen fluid samples from 40 Holstein dairy cows (on d 56 postpartum) with different rumen pH (2–4 h after morning feeding) were explored to investigate the difference of susceptibility to SARA and the correlation between microbiome, LPS and inflammation. These cows were categorized into low pH (LPH, pH ≤ 6.0, n = 20) and high pH (HPH, pH ≥ 6.5, n = 20) groups.ResultsThe results showed that LPH group increased the concentrations of total volatile fatty acids, acetate, propionate, butyrate and valerate. However, milk yield and milk compositions were unaffected. Compared to the HPH group, the LPH group increased the concentrations of serum BHBA, NEFA, LPS, HIS, IL-2, IL-6, TNF-α, and MDA, and decreased the concentrations of serum IgA, IgM, IgG, SOD, T-AOC, and mTOR. In addition, the LPH group decreased the copies of Ruminococcus flavefaciens and increased the copies of Fibrobacter succinogenes. Microbial community analysis isupplendicated a significant difference in bacterial composition between the two groups. At the phylum level, Bacteroidota and Firmicutes were enriched in the LPH and HPH groups, respectively. At the genus level, the dominant bacteria in the LPH group were Prevotella. Additionally, the LPH group increased the proportions of Gram-negative phenotypes, potentially pathogenic phenotypes and LPS biosynthesis. The close correlation between two key enzymes for LPS synthesis LpxL and LpxM with rumen pH, inflammatory markers, and microorganisms indicates that low pH may increase the risk of inflammation by facilitating the lysis of Gram-negative bacteria and the release of penta-acylated LPS. Penta-acylated and hexa-acylated LPS may be mainly derived from Prevotella and Succinivibrionaceae_UCG-001, respectively.DiscussionOverall, these results support the notion that transient low pH could reflect the risk of cows suffering from SARA and associated inflammation and is strongly associated with penta-acylated LPS. Our findings provide new insights into ruminant health improvement and disease prevention strategies.