bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful delivery of CRISPR-Cas9 with a baculovirus vector for insect brain targets
Authors: Doğan Büşra Elif Kıvrak , Ghanem Saleh , Goblirsch Michael , Nazneen Farzana , Broadrup Robert L. , Connolly-Sporing Annie , Tuncer Doğa , Mayack Christopher 
Published: November 2024

SummaryCRISPR-Cas9 (clustered, regularly interspaced, short palindromic repeats with CRISPR-associated protein 9) is a powerful, versatile, and cost-effective molecular tool that can be used for genetic engineering purposes and beyond1and is especially suited for non-model organisms2. Effective delivery of this system, however, remains a challenge forin vivogenetic manipulation of specific tissues3, particularly the brain4, and in adult indivuduals5,6. We designed a new CRISPR-Cas9 plasmid that was inserted into a baculovirus vector to knockdown theoctopamine beta subtype 2 receptor(AmOctβ2R), a transmembrane protein found in the mushroom body neurons of the honey bee (Apis mellifera) brain, to determine if octopamine plays a role in appetite regulation. We first confirmed that gene editing ofAmOctβ2Ris possible with Sanger sequencing. We then demonstrated expression of the CRISPR-Cas9 system with the baculovirus vectorin vitrousing live cell imaging, flow cytometry analysis, andin vivousing confocal imaging, showing widespread expression in the cells and throughout the honey bee brain, three days post treatment. There was alsoin vitroandin vivoknockdown ofAmOctβ2Rthree days post-infection, that corresponded with appetite suppression in starved forager bees. Our findings suggest that we successfully delivered the CRISPR-Cas9 system and knocked downAmOctB2Rin neuronal cells of the honey bee brain that were previously inaccessible due to the blood brain barrier and lack of infectivity of lentivirus vectors7. The newly characterizedAmOctβ2R8can now be assigned a functional role and other targets for gene editing are now possible using this CRISPR-Cas9 system.Graphical Abstract
Arabidopsis PRMT5 Buffers Pre-mRNA Splicing and Development Against Genetic Variation in Donor Splice Sites
Authors: Beckel Maximiliano S. , Martín Abril San , Sánchez Sabrina E. , Seymour Danelle K. , de Leone María José , Careno Daniel , Mora-García Santiago , Weigel Detlef , Yanovsky Marcelo J. , Chernomoretz Ariel 
Published: November 2024

ABSTRACTGenetic variation at splice site signals significantly influences alternative splicing, leading to transcriptomic and proteomic diversity that enhances phenotypic plasticity and adaptation. However, novel splice variants can negatively impact gene expression and developmental stability. Canalization—the ability of an organism to maintain a consistent phenotype despite genetic or environmental variations—helps balance the effects of genetic variation on development and evolution. Protein arginine methyltransferase 5 (PRMT5) is a key splicing regulator in plants and animals. Most splicing changes inprmt5mutants are linked to weak donor splice sites, suggesting that PRMT5 may buffer splicing against genetic variation. We examined PRMT5’s effects on splicing and development in two genetically divergentArabidopsis thalianaaccessions with different single nucleotide polymorphisms (SNPs) affecting donor splice sites. While PRMT5 inactivation similarly affected splicing in both backgrounds, it significantly increased splicing and phenotypic differences between the accessions. Our findings suggest that PRMT5 contributes to canalization, mitigating the impact of splice site polymorphisms and facilitating the evolution of adaptive splicing patterns.
Synthetic ratio computation for programming population composition and multicellular morphology
Authors: An Bolin , Tang Tzu-Chieh , Zhang Qian , Wang Teng , Gan Kesheng , Liu Kun , Liu Yuzhu , Wang Yanyi , Shaw William M. , Liang Qianyi , Wang Yaomin , Lu Timothy K. , Church George M. , Zhong Chao 
Published: November 2024

ABSTRACTRecent advancements in genetic engineering have provided diverse tools for artificially synthesizing population diversity in both prokaryotic and eukaryotic systems. However, achieving precise control over the ratios of multiple cell types within a population derived from a single founder remains a significant challenge. In this study, we introduce a suite of recombinase-mediated genetic devices designed to achieve accurate population ratio control, enabling the distribution of distinct functionalities across multiple cell types. We systematically evaluated key parameters influencing recombination efficiency and developed data-driven models to reliably predict binary differentiation outcomes. Using these devices, we implemented parallel and series circuit topologies to create user-defined, complex cell fate branching programs. These branching devices facilitated the autonomous differentiation of precision fermentation consortia from a single founder strain, optimizing cell-type ratios for applications such as pigmentation and cellulose degradation. Beyond biomanufacturing, we engineered multicellular aggregates with genetically encoded morphologies by coordinating self-organization through cell adhesion molecules (CAMs). Our work provides a comprehensive characterization of recombinase-based cell fate branching mechanisms and introduces a novel approach for the bottom-up, high-resolution construction of synthetic consortia and multicellular assemblies.
Annelid perspectives into the role of FGF signaling in caudal regeneration
Authors: Shalaeva Alexandra Y. , Kozin Vitaly V. 
Published: November 2024

AbstractFibroblast growth factor signaling plays a crucial role in various developmental processes and is a key driver of regeneration. In annelids this pathway is active from the earliest stages of reparative morphogenesis, yet its specific function remains unclear. Here we have functionally examined FGF signaling following the amputation of posterior segments in the marine annelidAlitta virens. We utilized the pharmacological agent SU5402 to inhibit the FGF receptor kinase at different time points. With whole-mount in situ hybridization we analyzed the expression of regulatory genes that pattern posterior territories (cdx, evx, post2), multipotent/germ cells (vasa, piwi), mesodermal tissues (twist), and segmental boundaries (engrailed). Our findings reveal that FGF signaling is essential for blastema induction by promoting dedifferentiation and proliferation of cells at the wound site but is not involved in posteriorization. On the contrary, this pathway is crucial for differentiation in the anterior part of the regenerative bud, impacting its mesodermal derivatives and segment boundary formation. Comparative analysis suggests that while certain functions of FGF signaling, particularly in mesodermal patterning, are conserved across taxa, its role in posterior axis elongation appears to have evolved specifically within the vertebrate lineage. This research enhances our understanding of the evolutionary origins and functional diversification of FGF signaling in regeneration, positioningA. virensas a valuable model for exploring the complexities of regenerative biology.
Structure of the Tilapia lake virus nucleoprotein bound to RNA
Authors: Arragain Benoît , Pelosse Martin , Huard Karine , Cusack Stephen 
Published: November 2024

ABSTRACTTilapia Lake virus (TiLV) belongs to theAmnoonviridaefamily within theArticulaviralesorder of segmented negative-strand RNA viruses and is highly diverged from more familiar orthomyxoviruses, such as influenza. The viral nucleoprotein (NP), a key component of the replication machinery, packages the viral genome into protective ribonucleoprotein particles. Here we describe the electron cryo-microscopy (cryo-EM) structure of TiLV-NP bound to RNA withinin vitroreconstituted, small ring-like, pseudo-symmetrical oligomers. Although TiLV-NP is considerably smaller than its influenza counterpart and unrelated in sequence, it maintains the same topology and domain organisation. This comprises a head and body domain between which is a negatively-charged groove where single-stranded RNA binds. In addition, an oligomerisation loop inserts into a hydrophobic pocket in the neighbouring NP, the flexible hinges of which allow considerable variability orientation of neighbouring NPs. Focussed cryo-EM maps (up to 2.9 Å resolution) unambiguously define the 5′ to 3′ direction of the bound RNA, confirmed by double stranded, A-form RNA regions that extrude out from some of the NP-NP interfaces. This is the first description of how RNA binds to an articulaviral NP and superposition with orthomyxoviral NPs suggest that the mode of RNA binding is likely conserved across theArticulaviralesorder.
The propensity of fructose to induce metabolic dysfunction is dependent on the baseline diet, length of the dietary exposure, and sex of the mice
Authors: Fadhul Taghreed , Park Se-Hyung , Ali Heba , Alsiraj Yasir , Wali Jibran A. , Simpson Stephen J. , Softic Samir 
Published: November 2024

AbstractBackground/ObjectivesNumerous studies have implicated high intake of sugar, particularly fructose, with the development of obesity and metabolic complications. On the other hand, fructose from fruits and vegetables has undisputed benefits for metabolic health. This paradox questions how the same fructose molecule can be associated with detrimental health effects in some studies and beneficial in others.MethodsTo answer this question, male and female mice were fed different normal chow diets and provided 30% fructose solution in water.ResultsFructose-supplemented male mice on the Boston Chow Diet (BCD=23% protein, 22% fat, 55% carbs) gained weight, developed glucose intolerance and hepatic steatosis. In contrast, male mice on the Lexington Chow Diet (LXD=24% protein, 18% fat, 58% carbs) did not gain weight, remained glucose tolerant, and had normal hepatic lipid content when supplemented with fructose. Furthermore, fructose-fed male mice on a Low-Fat Diet (LFD=20% protein, 10% fat, 70% carbs) didn’t gain weight, but once switched to the BCD, they gained weight, exhibited worsening liver steatosis, and more advanced hepatic insulin resistance. The effects of fructose are sex-dependent, as female mice didn’t gain weight and remained insulin-sensitive when given fructose on BCD, despite developing hepatic steatosis.ConclusionsThe differences in metabolic outcomes correlate with the propensity of the baseline diet to suppress hepatic ketohexokinase expression and the de novo lipogenesis pathway. This is likely driven by the dietary fat-to-carbohydrate ratio. Thus, metabolic dysfunction attributed to fructose intake is not a universal outcome; rather, it depends on the baseline diet, sex, and exposure length.
Slip flow between corotating disks with heat transfer
Authors: Turkyilmazoglu Mustafa 
Published: November 2024

Purpose
This study aims to explore the hydrodynamic and thermal behavior of an incompressible fluid flowing between uniformly corotating disks with finite radii. The narrow gap between the disks necessitates accounting for slip flow in the radial direction, departing from the classic no-slip model.

Design/methodology/approach
The author uses a perturbation approach and derives full analytical approximations to the Navier–Stokes and energy equations up to the second order. Higher-order truncations require significant numerical effort due to the complexity of the resulting expressions.

Findings
For the no-slip case, the momentum solutions perfectly match those found in the literature. The author then demonstrates the convergence of the series solutions with slip for selected specific parameter sets. Finally, the author investigates the impact of both slip and Reynolds number on the velocity field, pressure and temperature field between the inlet and outlet positions.

Originality/value
The key finding is that both factors lead to thinner momentum and thermal boundary layers within the corotating finite disk setup, resulting in cooler disk surfaces.
“A new or false hope?” practice and policy on teaching–research linkage in contemporary Cambodia’s higher education
Authors: Nhem Davut 
Published: November 2024

PurposeThe teaching–research nexus (TRN) has assumed a prominent role in global higher education systems. However, the connection between the two domains has been subject to diverse interpretations within well-developed higher education systems. Little is known about translating TRN into policy and practice in diverse higher education spaces. In this regard, this article examines the recent trajectory and challenges associated with integrating research and teaching in Cambodia.Design/methodology/approachTo analyse the current development of TRN and its associated challenges, this article consolidates two sets of data: recent research papers and government policy documents on TRN in the last 10 years or so.FindingsThe analysis shows that the connection between teaching and research appears to be patchy in practice, as evidenced in previous studies. The recent government policies and interventions, although potentially fostering progress in research activities, pose dilemmas regarding the orientation and feasibility of linking these two domains.Research limitations/implicationsThis article focuses on enhancing TRN in Cambodia, one of the developing higher education contexts, thereby offering insights into the strategies and concerns within such environments. Policymakers in similar higher education contexts might benefit from considering the concerns and solutions highlighted in this article.Originality/valueResearch on policies and strategies for strengthening TRN in developing higher education contexts remains limited in the literature. This article contributes to our collective knowledge of TRN by looking at Cambodia's contemporary reality and strategies.
Enhancing investor decision-making: a comprehensive study of information disseminated on stock exchange websites
Authors: Rzem Marouane 
Published: November 2024

Purpose
Results from this study include a tool for financial literacy through the use of stock exchange websites and a framework for managing stock market information.

Design/methodology/approach
Prior research suggests that information can significantly influence judgment and decision-making. This paper aims to provide guidance on the characteristics of information disseminated on stock exchange websites and demonstrate the value of these platforms in informing and educating cyber-investors. Based on an exploratory study, the author proposes that information should be examined through three dimensions: nature, support and source. These dimensions are crucial in shaping the behavioral learning of cyber-investors. The study results include a tool for enhancing financial literacy via stock exchange websites and a framework for effectively managing stock market information.

Findings
This study identifies potential areas where improvements can be made by stock exchange Web designers and asset management platforms, particularly life insurance websites. Based on our results and to have a successful and attractive website for cyber-investors, designers must pay close attention to the layout of the website, its navigability, structure, colors and general ergonomics. A good match between these elements and the needs of potential investors will improve the conditions for finding relevant information on stock market websites. Furthermore, ease of access to information contributes to greater satisfaction and loyalty. This finding is in line with Eltkhtash (2013) and Syaeid (2019) who highlight the importance of ease of access to the site.

Originality/value
The exploratory study analyzes information available on four French stock exchange websites: Boursorama, Zonebourse, Abcbourse and Boursier.com. The study offers a set of knowledge that allows cyber-investors to make effective decisions using financial sources. This helps the consultation of stock exchange websites and enables to target the relevant information which provides a more accurate assessment of the evolution of financial assets. Based on our analysis, it appears that investors are more interested in certain criteria at the expense of other information.
Systematic review of modelling techniques in carbon trading research in construction
Authors: Kukah Augustine Senanu Komla , Jin Xiaohua , Osei-Kyei Robert , Perera Srinath 
Published: November 2024

Purpose
Carbon emissions trading is an effective instrument in reducing greenhouse gas emissions. There is a notable scarcity of comprehensive reviews on the modelling techniques within carbon trading research in construction.

Design/methodology/approach
This paper reviews 68 relevant articles published in 19 peer-reviewed journals using systematic search. Scientometric analysis and content analysis are undertaken.

Findings
Generally, China was the largest contributor to carbon trading research using quantitative models (representing 36% of the total articles). From the results, the modelling techniques identified were multi-objective grasshopper optimisation algorithm; system dynamics; interpretive structural modelling; multi-agent-based model; decision-support model; multi-objective chaotic sine cosine algorithm; optimised backpropagation neural network; sequential panel selection method; Granger causality test; and impulse response analysis. Moreover, the advantages and disadvantages of these techniques were identified. System dynamics was recommended as the most suitable modelling technique for carbon trading in construction.

Originality/value
This study is significant, and through this review paper, practitioners can easily be more familiar with the significant modelling techniques, and this will motivate them to better understand their uses.
Compassion-Based Resilience Training (CBRT) to protect and nurture community care providers
Authors: Tosatti Elise , Khamash Lana 
Published: November 2024

Purpose
This case study describes pilot implementation of Nalanda Institute’s Compassion-Based Resilience Training (CBRT) with community care providers in New York City (NYC) to protect them from stress and trauma impacts and nurture their capacity to deliver skillful, compassionate care.

Design/methodology/approach
The Academy for Community Behavioral Health in NYC offered CBRT at no cost to a diverse cohort of 28 nonprofit care providers. This virtual eight-week course met weekly for 2 hours over Zoom, with formal and informal meditation practice between sessions. A mixed methods pre/post survey assessed changes in meditation practice, work-related stress and mindfulness and compassion. A final survey invited participant feedback on course experiences and course outcomes.

Findings
This case study suggests that a virtual eight-week CBRT course can be both feasible and effective for community care providers, even in their demanding roles. Most participants who started the course completed it (77%). CBRT helped participants establish a regular meditation practice and led to improvements on indicators of work-related stress, mindfulness and compassion. Participants linked these changes to an increased sense of hopefulness, agency and resilience for navigating stress and trauma in their work. They also found valued peer support in the course.

Originality/value
This case study offers one model for sharing effective but underused contemplative healing tools with care providers who encounter significant stress and trauma. It can inform efforts by funders, organizational leaders, managers, direct care staff and researchers to better protect these critical helpers.
Unexpected good news: the impacts of land decentralization reform on energy development in China
Authors: Sun Tianshi , Wang Mingyue 
Published: November 2024

PurposeThe purpose of this paper is to investigate the impact of land approval decentralization on energy efficiency and then analyze the mechanisms that land decentralization improves energy efficiency.Design/methodology/approachThis paper has been based on the dynamic slack based measure (DSBM) method to measure energy efficiency using input-output data at the city level in China. In the empirical analysis, this paper employed difference in differences (DID), instrumental variable (IV), propensity score matching–difference in differences (PSM–DID) and triple differences (DDD) to examine the research hypotheses.FindingsFirstly, this paper finds unexpected good news and shows that decentralizing land approvals significantly improves regional energy efficiency. Secondly, this paper explores two mechanisms for this effect, one is the mitigation of land misallocation and the other one is the cleaner allocation of land resources across industries. Thirdly, this paper provides micro-evidence from Chinese listed firms and finds that the decentralization of land approvals similarly improve firms’ energy efficiency.Originality/valueExisting studies have mainly explored the environmental impacts of land marketization reforms, with limited attention to decentralization of land approval. As a result, the promotion effect of decentralization of land approval on energy efficiency is still unclear, and the mechanism need to be further refined. Based on empirical analysis, this paper not only helps to clarify the relationship between land decentralization reform and energy efficiency, but also explores the mechanisms of transmission.
Staffing rightsizing: analyzing staffing studies to inform potential rightsizing practices
Authors: Baril Kathleen 
Published: November 2024

PurposeA review of the literature on how academic library staffing is adjusted is especially relevant right now, as higher education is anticipating a large decline in college-age students matriculating into colleges and universities beginning in 2025. Academic libraries will be affected by these enrollment drops and will have to carefully consider how to support their institutions with smaller budgets and smaller staff sizes. This review will provide libraries with potential strategies to utilize as they consider how best to serve their users, both with changing demographics and continued changes in technology.Design/methodology/approachThe literature on staffing in academic libraries was reviewed from the last 20 years in the context of relevance to rightsizing.FindingsFindings show that there are studies looking at staffing trends over longer periods of time, analyses of particular areas (i.e. reference desks, technical services, etc.) to determine levels and types of staff needed (librarians, library staff or student workers), financial analyses of staffing, studies on outsourcing and a few studies on the dangers of downsizing.Research limitations/implicationsSince the research is limited to the library literature, the findings could potentially be limited. In addition, not all staffing studies were deemed relevant to the concept of rightsizing, so this study is not all inclusive.Originality/valueThere are many publications on staffing, but the researcher did not find a literature review of staffing studies from a rightsizing perspective.