bluuebunny/crossref_metadata_2025_split huggingface dataset:
Escherichia coli inner membrane display system for high‐throughput screening of dimeric proteins
Authors: Jo Migyeong , Hwang Bora , Yoon Hyun Woung , Jung Sang Taek 
Published: September 2018

AbstractMultimer formation is indispensable to the intrinsicbiologicalfunctions of many natural proteins. For example, the human immunoglobulin G (IgG) antibody has two variable regions (heavy chain variable domain [VH] and light chain variable domain [VL]) that must be assembled for specific antigen binding, and homodimerization of the antibody's Fc domain is essential for eliciting therapeutic effector functions. For the more efficient high‐throughput directed evolution of multimeric proteins with ease of cultivation and handling, here we report a membrane protein drift and assembly (MPDA) system, in which a multimeric protein is displayed on a bacterial inner membrane by drifting and auto‐assembling membrane‐anchored subunit polypeptides. This system enabled the auto‐assembly of membrane‐tethered Fv domains (VH and VL) or the monomeric Fc domain into a functional hetero‐ or homodimeric protein complex on the bacterial inner membrane. This system could also be used to enrich a desired engineered Fc variant from a mixture containing a million‐fold excess of wild‐type Fc domain, indicating the applicability of the MPDA system for the high‐throughput directed evolution of a variety of multimeric proteins, such as cytokines, enzymes, or structural proteins.
Coassembly of a Polyoxometalate and a Zwitterionic Amphiphile into a Luminescent Hydrogel with Excellent Stimuli Responsiveness
Authors: Wu Aoli , Sun Panpan , Sun Na , Yu Yang , Zheng Liqiang 
Published: September 2018

AbstractThe co‐existence of electrostatic attraction and repulsion between zwitterionic amphiphile and anionic polyoxometalate (POM) promotes the formation of aqueous inorganic–organic coassemblies. Injectable, luminescent supramolecular hydrogels have been fabricated by using this novel and versatile technique. Hydrogels with different characteristics can be flexibly manipulated by adjusting the molar ratios of compounds. Interestingly, a linear relationship between the luminescence intensity and temperature accompanying the sol‐gel transition was observed. In addition, the emission properties of the hydrogels were sensitive to external acid and base gasses. Such multi‐stimuli responsive luminescent hydrogels provide an attractive pathway to construct intelligent optical soft materials, especially for biological sensors for which stimuli responsiveness is necessary.
Septin mutations and phenotypes in S. cerevisiae
Authors: Mela Alexander , Momany Michelle 
Published: September 2018

AbstractSeptins are highly conserved guanosine triphosphate (GTP)‐binding proteins that are a component of the cytoskeletal systems of virtually all eukaryotes (except higher plants). Septins play important roles in a multitude of cellular processes, including cytokinesis, establishment of cell polarity, and cellular partitioning. The ease of genetic screens and a fully sequenced genome have made Saccharomyces cerevisiae one of the most extensively studied and well‐annotated model organisms in eukaryotic biology. Here, we present a synopsis of the known point mutations in the seven S. cerevisiae septin genes: CDC3, CDC10, CDC11, CDC12, SHS1, SPR3, and SPR28. We map these mutations onto septin protein structures, highlighting important conserved motifs, and relating the functional consequences of mutations in each domain.
Small Heat Shock Proteins B1 and B6: Which One is the Most Effective Adjuvant in Therapeutic HPV Vaccine?
Authors: Bolhassani Azam , Shahbazi Sepideh , Milani Alireza , Nadji Seyed Alireza 
Published: September 2018

AbstractTherapeutic human papillomaviruse (HPV) vaccines have the potential to inhibit the tumor growth by targeting HPV E6 and E7 oncoproteins. Among different vaccine strategies, DNA and protein‐based approaches are the most effective candidates for stimulation of the immune responses against HPV infections. Our study was designed to assess the efficacy of small heat shock proteins B1 (Hsp27) and B6 (Hsp20) as an adjuvant accompanied by HPV16 E7 and hPP10‐E7 antigens in tumor mouse model. A major key for successful DNA and protein transfer into cells is the development of delivery systems with high efficiency and low cytotoxicity. Herein, we used hPP10 and MPG cell penetrating peptides (CPPs) for protein and DNA delivery in vivo, respectively. Our data indicated that the combination of Hsp27 with the recombinant hPP10‐E7 protein in homologous protein/protein (hPP10‐E7 + Hsp27) and heterologous DNA/protein (pcDNA‐E7 + MPG/ hPP10‐E7 + Hsp27) significantly enhanced the E7‐specific T cell responses. Indeed, these regimens induced high levels of IgG2a, IFN‐γ and IL‐2 directed toward Th1 responses and also Granzyme B secretion as compared to other immunization strategies, and also displayed complete protection more than 60 days after treatment. These data suggest that the use of Hsp27 as an adjuvant and MPG and hPP10 as a gene and protein carrier would represent promising applications for improvement of HPV therapeutic vaccines. © 2018 IUBMB Life, 70(10):1002–1011, 2018
LncRNA NKILA correlates with the malignant status and serves as a tumor‐suppressive role in rectal cancer
Authors: Tao Fei , Xu Yongpan , Yang Dezhen , Tian Bo , Jia Yong , Hou Junming , Dong Ming 
Published: September 2018

AbstractNF‐κB interacting lncRNA (NKILA) has been found to function as a tumor‐suppressive role in various human cancers. However, the role of NKILA in rectal cancer is still unknown. The objective of this study is to investigate the clinical value and biological function of NKILA in rectal cancer. The association between NKILA expression and clinical variables including prognosis was estimated in rectal cancer patients. The gain‐of‐function study of NKILA in rectal cancer cell was conducted to evaluate the effect of NKILA on cell proliferation, migration, invasion, and NF‐κB signaling pathway. The results suggested NKILA expression was decreased in rectal cancer tissues and cells, and correlated with clinical stage, T classification, N classification and M classification. NKILA low‐expression was an independent poor prognostic factor in rectal cancer patients. NKILA‐inhibited rectal cancer cell proliferation, migration, and invasion via suppressing NF‐κB signaling. In conclusion, NKILA serves as an antioncogenic lncRNA in rectal cancer.
Synthesis and DNase I inhibitory properties of some 4‐thiazolidinone derivatives
Authors: Kolarević Ana , Ilić Budimir S. , Kocić Gordana , Džambaski Zdravko , Šmelcerović Andrija , Bondžić Bojan P. 
Published: September 2018

AbstractTwelve new thiazolidinones were synthesized and, together with 41 previously synthesized thiazolidinones, evaluated for inhibitory activity against deoxyribonuclease I (DNase I) in vitro. Ten compounds inhibited commercial bovine pancreatic DNase I with an IC50 below 200 μM and showed to be more potent DNase I inhibitors than crystal violet (IC50 = 365.90 ± 47.33 μM), used as a positive control. Moreover, three compounds were active against DNase I in rat liver homogenate, having an IC50 below 200 μM. (3‐Methyl‐1,4‐dioxothiazolidin‐2‐ylidene)‐N‐(2‐phenylethyl)ethanamide (41) exhibited the most potent DNase I inhibition against both commercial and rat liver DNase I with IC50 values of 115.96 ± 11.70 and 151.36 ± 15.85 μM, respectively. Site Finder and molecular docking defined the thiazolidinones interactions with the most important catalytic residues of DNase I, including the H‐acceptor interaction with residues His 134 and His 252 and/or H‐donor interaction with residues Glu 39 and Asp 168. The three most active compounds against both commercial and rat liver DNase I (31, 38, and 41) exhibited favorable physico‐chemical, pharmacokinetic, and toxicological properties. These observations could be utilized to guide the rational design and optimization of novel thiazolidinone inhibitors. Thiazolidinones as novel DNase I inhibitors could have potential therapeutic applications due to the significant involvement of DNase I in the pathophysiology of many disease conditions.
Differential proteomic profiling identifies novel molecular targets of pterostilbene against experimental diabetes
Authors: Dornadula Sireesh , Thiruppathi Suresh , Palanisamy Rajaguru , Umapathy Dhamodharan , Suzuki Takayoshi , K. Mohanram Ramkumar 
Published: September 2018

AbstractPterostilbene (PTS), a naturally occurring stilbene, confers protection against oxidative and cytokine stress induced pancreatic β‐cell apoptosis in vitro and in vivo. To provide insights into the molecular mechanism, we performed a proteomic study on the pancreas of PTS‐treated diabetic mice using electrospray ionization tandem–mass spectrometry (LC–MS/MS). A total of 1,260 proteins were detected in triplicate samples. Of which, 359 proteins were found to be differentially regulated in streptozotocin‐induced diabetic mice pancreas with two fold difference (
P < 0.05, two or more peptides) and on PTS treatment 315 proteins were normalized to control levels. Gene ontology (GO) indicated that majority of the differentially regulated proteins are involved in cellular functions such as metabolism, cellular structure, oxidative stress, endoplasmic‐reticulum‐associated protein degradation (ERAD) pathway and several stress sensors. Protein–protein interaction network analysis of these differentially expressed proteins showed clustering of proteins involved in protein processing in endoplasmic reticulum (protein synthesis machinery and protein folding), oxidative phosphorylation/oxidative stress proteins, oligosaccharide metabolic process, and antioxidant activity. Our results highlighted that PTS administration rehabilitated the defective metabolic process and redox imbalance, and also suppressed the unfolded protein response and ERAD pathways. The effects on targeting ER machinery and suppressing oxidative stress suggest the great potential of PTS for diabetes management.
Oxidative, inflammatory, genetic, and epigenetic biomarkers associated with chronic obstructive pulmonary disorder
Authors: Aggarwal Taru , Wadhwa Ridhima , Thapliyal Noopur , Sharma Kanishka , Rani Varsha , Maurya Pawan K. 
Published: September 2018

AbstractA large body of evidence indicates that chronic obstructive pulmonary disorder (COPD) is accompanied by oxidative stress and inflammatory and genetic pathways. Epidemiological studies indicate that COPD is a major cause of mortality and morbidity in the world. Recent research development in COPD focuses on accelerated aging and various oxidative stress biomarkers. It involves the clinical manifestation of the disease process and may also contain biochemical, immunological, physiological, morphological, and genetic aspects that add to the progressiveness of the disease. Herein, we summarize findings that highlight the role of dimensions of COPD in the investigation of oxidative stress, inflammatory responses, genetic and epigenetic studies, and pharmacological and dietary antioxidant intervention.
Ultrasound‐Guided Prolotherapy for Acromial Enthesopathy and Acromioclavicular Joint Arthropathy: A Single‐Arm Prospective Study
Authors: Hsieh Pei‐Chun , Chiou Hong‐Jen , Wang Hsin‐Kai , Lai Yi‐Chen , Lin Yung‐Hui 
Published: September 2018

ObjectivesProlotherapy is an injection‐based complementary treatment for various musculoskeletal diseases. The aim of this study was to evaluate the therapeutic efficacy of ultrasound‐guided prolotherapy in the treatment of acromial enthesopathy and acromioclavicular joint arthropathy.MethodsThirty‐one patients with chronic moderate‐to‐severe shoulder pain were recruited from September 2015 to September 2017. Ultrasound‐guided prolotherapy was performed by injecting 10 mL of a 15% dextrose solution into the acromial enthesis of the deltoid or acromioclavicular joint capsule aseptically. Prolotherapy was given in 2 sessions separated by a 1‐month interval. The pretreatment‐to‐posttreatment change in the pain visual analog scale (VAS) score was recorded as the primary outcome. The mean follow‐up duration was 61.8 days. A paired t test was used to assess the difference in pretreatment and posttreatment VAS scores. A univariate logistic regression analysis was conducted to identify the demographic variables associated with substantial pain reduction after the intervention. Substantial pain reduction was defined as a posttreatment VAS score of 3 or less.ResultsTwenty of the 31 patients reported substantial pain reduction without adverse effects after the intervention. The mean VAS score reduction ± SD was 4.3 ± 2.6 (pretreatment, 6.8 ± 1.5; posttreatment, 2.5 ± 2.1; P < .01).ConclusionsUltrasound‐guided prolotherapy with a 15% dextrose solution is an effective and safe therapeutic option for moderate‐to‐severe acromial enthesopathy and acromioclavicular joint arthropathy.
Difference in Visceral Adipose Tissue in Pregnancy and Postpartum and Related Changes in Maternal Insulin Resistance
Authors: Shinar Shiri , Berger Howard , De Souza Leanne R. , Ray Joel G. 
Published: September 2018

ObjectiveTo measure the difference between first‐trimester and postpartum visceral adipose tissue (VAT), the agreement of this difference with change in body mass index, and whether a difference in VAT is associated with insulin resistance or glucose mishandling.MethodsProspective study of 93 women with singleton pregnancies without a history of diabetes. Visceral adipose tissue depth was sonographically assessed at 11 to 14 weeks and at 6 to 12 weeks postpartum. Metabolic measures, sampled at 24 to 28 weeks and 6 to 12 weeks postpartum, included homeostatic model assessment of insulin resistance, insulin sensitivity index composite, and area under the 75‐g oral glucose tolerance test curve.ResultsFirst‐trimester VAT depth explained only 37% (95% confidence interval [CI], 22–52) of the variation in postpartum VAT depth. There was limited agreement between the net change in postpartum minus first‐trimester VAT depth and that same net change for body mass index (Cohen's kappa, 0.26; 95% CI, 0.05–0.47). Those with a net gain in VAT depth demonstrated poorer insulin sensitivity index postpartum than women with a net regression in VAT depth—a difference of –2.0 (95% CI, –3.3 to –0.69).ConclusionSonographic assessment of postpartum VAT is feasible and may provide insight to metabolic changes between pregnancy and postpartum, beyond body mass index.
Diagnostic Accuracy of 2‐Dimensional Shear Wave Elastography for the Staging of Liver Fibrosis: A Meta‐analysis
Authors: Zhang Wei , Zhu Ya , Zhang Cuncheng , Ran Haitao 
Published: September 2018

ObjectivesTo evaluate the overall accuracy of 2‐dimensional shear wave elastography (SWE) for the staging of liver fibrosis.MethodsLiterature databases and conference abstracts were searched from 2000 to September 2017. Sensitivity, specificity, and other information were extracted from the included studies. Methodological quality was assessed with Quality Assessment of Diagnostic Accuracy Studies 2 tools. Data were synthesized by a bivariate hierarchical model.ResultsThe summary sensitivity and specificity were 0.85 (95% confidence interval [CI], 0.80–0.89) and 0.79 (95% CI, 0.72–0.85) for fibrosis stage F≥2, 0.90 (95% CI, 0.87–0.93) and 0.85 (95% CI, 0.80–0.89) for F≥3, and 0.89 (95% CI, 0.84–0.93) and 0.92 (95% CI, 0.89–0.95) for F=4, respectively. The areas under the summary receiver operating characteristic curve for F≥2, F≥3, and F=4 was 0.81, 0.77, and 0.84.ConclusionsTwo‐dimensional SWE is a good noninvasive method for the diagnosis of substantial liver fibrosis and cirrhosis. Further studies are needed to assess severe fibrosis and to perform head‐to‐head comparisons of 2‐dimensional SWE and other imaging modalities for the evaluation of liver fibrosis.
Quantification of Urinary Loss in Nulliparous Athletes During 1 Hour of Sports Training
Authors: dos Santos Keyla M. , Da Roza Thuane , Tonon da Luz Soraia C. , Hort Julia P. , Kruger Joyce M. , Schevchenco Bruna 
Published: September 2018

BackgroundUrinary incontinence (UI) is a pelvic floor dysfunction that can affect nulliparous female athletes because of the effect of sports activities on pelvic floor muscles.ObjectivesTo verify and quantify urine loss in nulliparous athletes during 1 hour of sports training using a modified pad test protocol.DesignCross‐sectional.SettingSecondary, institutional practice.ParticipantsNulliparous athletes (N = 104).MethodsAthletes completed the International Consultation on Incontinence Questionnaire‐Short Form (ICIQ‐UI‐SF) and underwent a maximal resistance test of the abdominal muscles followed by the new modified pad test during 1 hour of training.OutcomesPresence or absence of UI was the dependent variable; the initial hypothesis was that high‐impact activities could lead to the development of UI.ResultsAlmost 52% of athletes (n = 54) self‐reported UI according to the ICIQ‐UI‐SF score. Of athletes who reported stress or mixed UI (n = 32), only 43.7% (n = 14) had leakage during the training pad test. Interestingly, 24% of athletes (n = 12) who did not report UI had a positive pad test result. In total, 27.9% of athletes (n = 29) presented a positive pad test during the training. Mean urinary loss was 1.57 ± 0.4 g.ConclusionAthletes did not seem to have a good knowledge of UI symptoms, and the modified pad test could be an alternative to quantify urine loss; however, it is necessary to perform accuracy tests. None of the athletes reported discomfort or decreased performance when performing the pad test.Level of EvidenceII
Experimental Poroviscoelasticity of Common Sedimentary Rocks
Authors: Makhnenko Roman Y. , Podladchikov Yury Y. 
Published: September 2018

AbstractThe success of geoenergy applications such as petroleum recovery or geological storage of CO2 depends on properly addressing the physical coupling between the pore fluid diffusion and mechanical deformation of the subsurface rock. Constitutive models should include short‐term hydromechanical interactions and long‐term behavior and should incorporate the principles behind the mathematical models for poroelastic and poroviscoelastic responses. However, the viscous parameters in constitutive relationships still need to be validated and estimated. In this work, we experimentally quantify the time‐dependent response of fluid‐filled sedimentary rocks at room temperature and isotropic stress states. Drained, undrained, and unjacketed geomechanical tests are performed to measure the poroelastic parameters for Berea sandstone, Apulian limestone, clay‐rich material, and Opalinus clay (shale). A poroviscous model parameter, the bulk viscosity, is included in the constitutive relationships. The bulk viscosity is estimated under constant isotropic stress conditions from time‐dependent deformation of rock in the drained regime for timescales ~105 s and from observations of the pore pressure growth under undrained conditions at timescales of ~104 s. The bulk viscosity is on the order of 1015–1016 Pa s for sandstone, limestone, and shale and ~1013 Pa s for clay‐rich material, and it decreases with an increase in pore pressure despite a corresponding decrease in the effective stress. In the long term, fluid pressure can asymptotically approach minimum principal stress, which in natural reservoirs may lead to liquefaction or rock embrittlement, causing slip instabilities and earthquakes and creating high‐permeability channels in low‐permeable rock.
Multiple resonant controllers strategy to achieve fault ride‐through and high performance output voltage in UPS applications
Authors: Carballo Roberto Esteban , Botterón Fernando , Oggier Germán Gustavo , García Guillermo Oscar 
Published: September 2018

A control strategy to achieve fault ride‐through capability and to provide high performance of the output voltage of a single‐phase uninterruptible power supply (UPS) inverter is proposed in this study. This strategy consists in controlling the voltage and current waveforms measured at the inverter output filter, with an inner current control loop and an outer voltage control loop, using a plug‐in structure based on multiple resonant stages in addition with proportional controllers. In order to achieve stability from no load to short‐circuit conditions, the implementation of the multiple resonant controllers includes a compensation of the system phase lag. Moreover, it presents a comparative analysis between two controller structures, the proposed plug‐in and the classical proportional + resonant. From this analysis, it can be concluded that the plug‐in structure presents improved characteristics of closed‐loop output impedance and output voltage dynamic response during fault ride‐through events. A controller design methodology to achieve robustness to parametric uncertainties and UPS standard compliance, is detailed. Experimental results from a single‐phase 2 kVA inverter prototype are presented to validate the feasibility of the proposal.
An examination of opioid prescription for Medicare Part D patients among family practice prescribers
Authors: Pan Kevin , Blankley Alan I , Hughes Peter J 
Published: September 2018

Abstract

Background
In the USA, opioid overdose accounted for more than 60% of drug overdose deaths in 2015. Of these deaths, 40% were due to use of prescription opioids.

Objectives
The aims of the study were to (i) study family medicine physician opioid-prescribing rate and duration of prescription, (ii) study the distribution of prescription by medication potency, (iii) study opioid-prescribing trends in health care shortage areas and (iv) study the association between extreme high prescribing rates and medical board discipline.

Methods
This is a retrospective cross-sectional study of the 2015 Medicare Part D claim data.

Results
Family practitioners have opioid prescription rates (5.6%) similar to medical subspecialists (6.0%), but lower than pain specialists (53.2%) and surgical specialists (36.6%). Family practitioners have an average opioid prescription duration (21.5 days) similar to medical subspecialists (23.1 days) and pain specialists (27.1 days), but longer than surgical specialists (8.9 days). Family practitioners tend to prescribe lower potency opioids. Family practitioners in rural health care shortage areas have a higher opioid prescription rate than other family practitioners (6.5% versus 5.6%). Among the 52 family practitioners who prescribed opioids as frequently as pain specialists, 26 of the 52 (50%) were certified in pain management or worked with a partner certified in pain management. Of the other 26 family practitioners, 3 (12%) had medical board disciplinary actions regarding opioid prescription.

Conclusions
While monitoring extreme prescribers is important and needs to be continued, the next step in policies to reduce prescription opioids will require systemic change, especially providing support for family practitioners in rural health care shortage areas.
Tensor Diagrams and Chebyshev Polynomials
Authors: Lamberti Lisa 
Published: September 2018

Abstract
In this paper, we describe a class of elements in the ring of $\textrm{SL}(V)$-invariant polynomial functions on the space of configurations of vectors and linear forms of a 3D vector space $V.$ These elements are related to one another by an induction formula using Chebyshev polynomials. We also investigate the relation between these polynomials and G. Lusztig’s dual canonical basis in tensor products of representations of $U_q(\mathfrak{sl}_3(\mathbb C)).$