bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Proteomic Analysis Provides Insights into the Regulatory Mechanisms of Wheat Primary Root Growth
Authors: Li Le , Xu Yanhua , Ren Yongzhe , Guo Zhanyong , Li Jingjing , Tong Yiping , Lin Tongbao , Cui Dangqun 
Published: August 2019

AbstractPlant roots are vital for acquiring nutrients and water from soil. However, the mechanisms regulating root growth in hexaploid wheat remain to be elucidated. Here, an integrated comparative proteome study on the roots of two varieties and their descendants with contrasting root phenotypes was performed. A total of 80 differentially expressed proteins (DEPs) associated with the regulation of primary root growth were identified, including two plant steroid biosynthesis related proteins and nine class III peroxidases. Real-time PCR analysis showed that brassinosteroid (BR) biosynthesis pathway was significantly elevated in long-root plants compared with those short-root plants. Moreover, O2.− and H2O2 were distributed abundantly in both the root meristematic and elongation zones of long root plants, but only in the meristematic zone of short-root plants. The differential distribution of reactive oxygen species (ROS) in the root tips of different genotypes may be caused by the differential expression of peroxidases. Taken together, our results suggest that the regulation of wheat primary root growth is closely related to BR biosynthesis pathway and BR-mediated ROS distribution.
Phase-sensitive interferometry of decorrelated speckle patterns
Authors: Spahr Hendrik , Pfäffle Clara , Burhan Sazan , Kutzner Lisa , Hilge Felix , Hüttmann Gereon , Hillmann Dierck 
Published: August 2019

AbstractPhase-sensitive coherent imaging exploits changes in the phases of backscattered light to observe tiny alterations of scattering structures or variations of the refractive index. But moving scatterers or a fluctuating refractive index decorrelate the phases and speckle patterns in the images. It is generally believed that once the speckle pattern has changed, the phases are scrambled and any meaningful phase difference to the original pattern is removed. As a consequence, diffusion and tissue motion that cannot be resolved, prevent phase-sensitive imaging of biological specimens. Here, we show that a phase comparison between decorrelated speckle patterns is still possible by utilizing a series of images acquired during decorrelation. The resulting evaluation scheme is mathematically equivalent to methods for astronomic imaging through the turbulent sky by speckle interferometry. We thus adopt the idea of speckle interferometry to phase-sensitive imaging in biological tissues and demonstrate its efficacy for simulated data and imaging of photoreceptor activity with phase-sensitive optical coherence tomography. We believe the described methods can be applied to many imaging modalities that use phase values for interferometry.
A Spectrally Tunable Dielectric Subwavelength Grating based Broadband Planar Light Concentrator
Authors: Elikkottil Ameen , Tahersima Mohammed H. , Gupta M. V. N. Surendra , Maiti Rishi , Sorger Volker J. , Pesala Bala 
Published: August 2019

AbstractEnergy consumption of buildings is increasing at a rapid pace due to urbanization, while net-zero energy buildings offer a green and sustainable solution. However, limited rooftop availability on multi-story buildings poses a challenge for large-scale integration of photovoltaics. Conventional silicon solar panels block visible light making them unfeasible to cover all the surfaces of a building. Here, we demonstrate a novel dielectric grating based planar light concentrator. We integrate this functional device onto a window glass transmitting visible light while simultaneously guiding near infrared (NIR) portion of sunlight to edges of the glass window where it is converted to electricity by a photovoltaic cell. Gratings are designed to guide NIR region and realize polarization independent performance. Experimentally, we observe 0.72% optical guiding efficiency in the NIR region (700–1000 nm), transmitting majority of the visible portion for natural room lighting. Integrating solar cell at the window edge, we find an electrical conversion efficiency of about 0.65% of NIR light with a 25 mm2 prototype. Major losses are coupling and guiding losses arising from non-uniformity in fabrication over a large area. Such a functional window combining energy generation, natural room lighting and heat load reduction could mitigate urban heat island effect in modern cities.
Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells
Authors: Macone Alberto , Masciarelli Silvia , Palombarini Federica , Quaglio Deborah , Boffi Alberto , Trabuco Matilde Cardoso , Baiocco Paola , Fazi Francesco , Bonamore Alessandra 
Published: August 2019

AbstractIn this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the “heavy duty” carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
Multicolor imaging in central serous chorioretinopathy – a quantitative and qualitative comparison with fundus autofluorescence
Authors: Govindahari Vishal , Singh Sumit Randhir , Rajesh Bindu , Gallego-Pinazo Roberto , Marco Rosa Dolz , Nair Dhanya V. , Nair Unni , Chhablani Jay 
Published: August 2019

AbstractCentral serous chorioretinopathy (CSCR) is characterised by choroidal hyperpermeability which results in neurosensory detachments (NSD) along with numerous retinal pigment epithelium (RPE) alterations such as RPE atrophy. Fundus autofluorescence (FAF) demonstrates the functionality of the RPE while multicolor imaging(MCI), by means of its three incident wavelengths, provides insight into clinical changes at various levels of the retina and choroid in CSCR. This study compares various clinical findings in CSCR (NSD, subretinal deposits, RPE atrophy, pigment epithelial detachments (PED) and pachyvessels) on the above mentioned imaging modalities both qualitatively and quantitatively. MCI showed higher mean cumulative area of RPE atrophic patches (6.3 ± 6.02 vs 5.7 ± 5.7 mm2, p = 0.046), PED (1.3 ± 1.4 vs 1.1 ± 1.2 mm2, p = 0.068) and NSD (17.2 ± 11.4 vs 15.7 ± 10.7 mm2, p = 0.033). MCI demonstrated better defined lesions (NSD, PED, RPE atrophy) and more number of eyes with PED and pachyvessels in comparison to FAF.Both investigations had a 100% sensitivity in detecting NSD and 100% specificity for sub retinal deposits. This study demonstrates the ability of MCI to quantitatively and qualitatively define various clinical features in CSCR and the advantages it holds over FAF. MCI can hence be considered as a useful imaging modality in documenting and monitoring various structural changes in eyes with CSCR.
Risk aversion in the adjustment of speed-accuracy tradeoff depending on time constraints
Authors: Onagawa Ryoji , Shinya Masahiro , Ota Keiji , Kudo Kazutoshi 
Published: August 2019

AbstractHumans are often required to make decisions under time constraints and to adjust speed-accuracy tradeoff (SAT) based on time constraints. Previous studies have investigated how humans adjust SAT depending on the time discount rate of expected gain. Although the expected gain of actions can be determined by both gain and probability, only situations where gain decreases over time have been tested. Considering the effect of risk on decision-making, the difference in time discount factors may modulate the response strategies for SAT, since temporal changes in variance of possible outcomes differ when gain or probability decreases over time. Here, we investigated the response strategies for SAT under different time discount factors. Participants were required to select one of the two options with different initial values in situations where the expected gain of options declined over time by a linear decrease in gain or probability. Comparison of response strategies between conditions revealed that response times in the gain condition were longer than those in the probability condition, possibly due to risk-aversion. These findings indicate the existence of common rules underpinning sensorimotor and economic decision-making.
Signature profile of cyclooxygenase-independent F2 series prostaglandins in C. elegans and their role in sperm motility
Authors: Tiwary Ekta , Hu Muhan , Miller Michael A. , Prasain Jeevan K. 
Published: August 2019

AbstractWe previously discovered that Caenorhabditis elegans synthesizes Cox-independent F-series prostaglandins (PGs). To delineate the Cox-independent prostaglandin pathways and evaluate their role in sperm motility in C. elegans, we developed a novel biochemical method for the rapid production of F-series PGs using arachidonic acid as the substrate and worm lysate as source of enzyme(s). Among the four F2-series PGs produced in the reaction, three of them were identified as 8-isoPGF2α, 5iPF2 VI, and PGF2α based on their retention times and MS/MS spectral comparison with standards using LC-MS/MS. PG production was not markedly affected by specific antioxidants, or Cox, Lox, and Cyp inhibitors, suggesting that these PGs are formed through a novel, biologically regulated mechanism in C. elegans. This study also assessed the ability of 8-isoPGF2α, 5iPF2 VI, PGF2α, and a mixture containing these PGs in a 0.5/0.08/1 ratio that reflects their synthetic composition to modulate sperm motility in fat-2 mutants. PGF2α and the PG mixture at 25 μM concentration significantly stimulated sperm velocity by 28% and 38%, whereas 8-isoPGF2α and 5iPF2 VI reduced the velocity by 21% and 30%, respectively, compared to vehicle control. These results indicate that the sperm motility effects of PGs are structure- and composition-dependent in C. elegans.
Multi-foci laser microfabrication of 3D polymeric scaffolds for stem cell expansion in regenerative medicine
Authors: Zandrini Tommaso , Shan Oumin , Parodi Valentina , Cerullo Giulio , Raimondi Manuela T. , Osellame Roberto 
Published: August 2019

AbstractHigh quality large scale fabrication of cellular scaffolds, with three-dimensional resolution comparable to cell size, is an important task to enable regenerative medicine applications with stem cells. We are using two-photon polymerization to produce our stem cell culture substrate called Nichoid, which we already demonstrated capable of stimulating cell proliferation while maintaining their stemness, without the need of dangerous additives. Parallelization of this technique can be achieved with the use of a spatial light modulator: here we show the results obtained combining this device with fast linear stages to produce Nichoid-covered substrates by two-photon polymerization. The well-polymerized structures confirm that this approach is particularly convenient for porous structures, and allows a significant time saving by a factor of almost five, with minor design adjustments. A Live & Dead assay was performed on mesenchymal stem cells cultured into the Nichoid microstructures in order to verify that no difference in cell viability is present, compared to microstructures fabricated by a single focus. This parallel setup opens the possibility to obtain a much larger number of microstructured substrates, that are essential to test new stem cell-based therapies. This approach can be also used for the fast fabrication of other kinds of cell culture devices.
Novel and traditional lipid profiles in Metabolic Syndrome reveal a high atherogenicity
Authors: Paredes Sílvia , Fonseca Liliana , Ribeiro Laura , Ramos Helena , Oliveira José Carlos , Palma Isabel 
Published: August 2019

AbstractLow-density-lipoprotein cholesterol (LDL-c) guides lipid-lowering therapy, although other lipid parameters could better reflect cardiovascular disease (CVD) risk. Discordance between these parameters and LDL-c has not been evaluated in metabolic syndrome (MetS) patients. We characterized a comprehensive lipid profile in 177 MetS patients. The 2016 ESC/EAS Guidelines for the Management of Dyslipidemias were used to define LDL-c targets. The atherogenic lipoprotein profile was compared in patients with LDL-c within and above the target. Only 34.4% (61) of patients had mean LDL-c levels within the guidelines and patients with LDL-c above target presented significantly elevated levels of Apolipoprotein B (ApoB), non-high-density lipoprotein cholesterol (non-HDL-c) and oxidized LDL-c. In patients with LDL-c within target, 25%, 31% and 49% presented levels above the recommended range for ApoB, non-HDL-c and oxidized LDL-c, respectively. Patients presented a strong association of LDL-c and non-HDL-c (r = 0.796), ApoB (r = 0.749) and oxidized LDL-c (r = 0.452). Similarly, non-HDL-c was strongly correlated with ApoB (r = 0.857) and oxidized-LDL-c (r = 0.555). The logistic regression model evidenced higher triglycerides and HDL-c and lower ApoB as predictors of having LDL-c within target. Reliance solely on LDL-c could result in missed opportunities for CVD risk reduction. ApoB, oxidized LDL-c, and particularly non-HDL-c, could be valuable parameters to estimate the CVD risk of MetS patients and have the potential to be targeted therapeutically.
Removal of Hepatitis B virus surface HBsAg and core HBcAg antigens using microbial fuel cells producing electricity from human urine
Authors: Pasternak Grzegorz , Greenman John , Ieropoulos Ioannis 
Published: August 2019

AbstractMicrobial electrochemical technology is emerging as an alternative way of treating waste and converting this directly to electricity. Intensive research on these systems is ongoing but it currently lacks the evaluation of possible environmental transmission of enteric viruses originating from the waste stream. In this study, for the first time we investigated this aspect by assessing the removal efficiency of hepatitis B core and surface antigens in cascades of continuous flow microbial fuel cells. The log-reduction (LR) of surface antigen (HBsAg) reached a maximum value of 1.86 ± 0.20 (98.6% reduction), which was similar to the open circuit control and degraded regardless of the recorded current. Core antigen (HBcAg) was much more resistant to treatment and the maximal LR was equal to 0.229 ± 0.028 (41.0% reduction). The highest LR rate observed for HBsAg was 4.66 ± 0.19 h−1 and for HBcAg 0.10 ± 0.01 h−1. Regression analysis revealed correlation between hydraulic retention time, power and redox potential on inactivation efficiency, also indicating electroactive behaviour of biofilm in open circuit control through the snorkel-effect. The results indicate that microbial electrochemical technologies may be successfully applied to reduce the risk of environmental transmission of hepatitis B virus but also open up the possibility of testing other viruses for wider implementation.
Absorption avoided resonance crossing of hybridization of silicon nanoparticles and gold nanoantennas
Authors: Yang Jhen-Hong , Yu Min-Wen , Chen Kuo-Ping 
Published: August 2019

AbstractThe near-field coupling between a high-refractive-index nanoparticle and gold nanoantennas is investigated theoretically. The absorption enhancement and also avoided resonance crossing in the absorption cross section spectra were observed with the hybridization system due to the coupling between the localized surface plasmon resonance of the gold nanoantennas and the magnetic dipole resonance of the silicon nanoparticle. By controlling the nanoparticle size or the separation distance, the near-field coupling can be tuned from the weak to the strong regime.
MRTF-A controls myofibroblastic differentiation of human multipotent stromal cells and their tumour-supporting function in xenograft models
Authors: Werner Sara , Lützkendorf Jana , Müller Thomas , Müller Lutz P. , Posern Guido 
Published: August 2019

AbstractTumour growth and metastatic colonization is strongly influenced by the tumour stroma, including cancer-associated fibroblasts (CAF). Multipotent mesenchymal stromal cells (MSC) are a possible source of CAF following myofibroblastic differentiation, and we have previously shown that MSC support tumour growth. Triggered by tumour cell-derived factors like transforming growth factor β1 (TGF-β1), myofibroblastic MSC differentiation is associated with the increased expression of markers including alpha smooth muscle actin (α-SMA). Here we show that myocardin-related transcription factor A (MRTF-A) plays an important role in myofibroblastic differentiation of primary human MSC in vitro and their tumour-supporting function in vivo. Recombinant TGF-β1 or tumour cell conditioned medium (TCM) elevated α-SMA, calponin 1 and collagen 1 A1 (COL1A1) amount on mRNA and protein level in MSC. This correlated with increased MRTF-A activity during MSC differentiation. MRTF-A knockdown by siRNA or shRNA impaired TGF-β1 and TCM induction of α-SMA and calponin 1, but not of COL1A1. Mixed xenograft experiments using HCT8 colorectal carcinoma cells and primary MSC of different donors revealed a significant reduction in tumour weight and volume upon MRTF-A knockdown in MSC. Our study suggests that MRTF-A is involved in the functional differentiation of MSC towards a tumour-promoting CAF phenotype in vivo.
Optimization of a reduced enzymatic reaction cascade for the production of L-alanine
Authors: Gmelch Tobias J. , Sperl Josef M. , Sieber Volker 
Published: August 2019

AbstractCell-free enzymatic reaction cascades combine the advantages of well-established in vitro biocatalysis with the power of multi-step in vivo pathways. The absence of a regulatory cell environment enables direct process control including methods for facile bottleneck identification and process optimization. Within this work, we developed a reduced, enzymatic reaction cascade for the direct production of L-alanine from D-glucose and ammonium sulfate. An efficient, activity based enzyme selection is demonstrated for the two branches of the cascade. The resulting redox neutral cascade is composed of a glucose dehydrogenase, two dihydroxyacid dehydratases, a keto-deoxy-aldolase, an aldehyde dehydrogenase and an L-alanine dehydrogenase. This artificial combination of purified biocatalysts eliminates the need for phosphorylation and only requires NAD as cofactor. We provide insight into in detail optimization of the process parameters applying a fluorescamine based L-alanine quantification assay. An optimized enzyme ratio and the necessary enzyme load were identified and together with the optimal concentrations of cofactor (NAD), ammonium and buffer yields of >95% for the main branch and of 8% for the side branch were achieved.
A qPCR expression assay of IFI44L gene differentiates viral from bacterial infections in febrile children
Authors: Gómez-Carballa Alberto , Cebey-López Miriam , Pardo-Seco Jacobo , Barral-Arca Ruth , Rivero-Calle Irene , Pischedda Sara , Currás-Tuala María José , Gómez-Rial José , Barros Francisco , Martinón-Torres Federico , Salas Antonio 
Published: August 2019

AbstractThe diagnosis of bacterial infections in hospital settings is currently performed using bacterial culture from sterile site, but they are lengthy and limited. Transcriptomic biomarkers are becoming promising tools for diagnosis with potential applicability in clinical settings. We evaluated a RT-qPCR assay for a 2-transcript host expression signature (FAM89A and IFI44L genes) inferred from microarray data that allow to differentiate between viral and bacterial infection in febrile children. This assay was able to discriminate viral from bacterial infections (P-value = 1.04 × 10−4; AUC = 92.2%; sensitivity = 90.9%; specificity = 85.7%) and showed very high reproducibility regardless of the reference gene(s) used to normalize the data. Unexpectedly, the monogenic IFI44L expression signature yielded better results than those obtained from the 2-transcript test (P-value = 3.59 × 10−5; AUC = 94.1%; sensitivity = 90.9%; specificity = 92.8%). We validated this IFI44L signature in previously published microarray and whole-transcriptome data from patients affected by different types of viral and bacterial infections, confirming that this gene alone differentiates between both groups, thus saving time, effort, and costs. Herein, we demonstrate that host expression microarray data can be successfully translated into a fast, highly accurate and relatively inexpensive in vitro assay that could be implemented in the clinical routine.
RORα suppresses interleukin-6-mediated hepatic acute phase response
Authors: Kim Ju-Yeon , Han Yong-Hyun , Nam Min-Woo , Kim Hyeon-Ji , Lee Mi-Ock 
Published: August 2019

AbstractAcute liver failure (ALF) is characterized by loss of liver function in response to sustained augmentation of the acute-phase response (APR) in the liver, which can progress even to death. Although the inflammatory interleukin-6 (IL-6)–axis is a crucial factor that drives the hepatic APR by releasing diverse acute-phase proteins (APPs), therapeutic strategies to block the IL-6–STAT3-mediated APR are not well developed. Here, we show that the nuclear receptor retinoic acid-related orphan receptor α (RORα) limits APR-mediated liver injury by inhibiting the hepatic IL-6–STAT3 signaling pathway. Administration of JC1-40, an RORα activator, diminished diethylnitrosamine-induced acute liver injury and repressed transcriptional expression of APPs such as CXCL1 and LCN2 in mice. IL-6-mediated activation of STAT3 was repressed after RORα activation by either adenoviral infusion of RORα or JC1-40 treatment in primary hepatocytes. Activation of RORα decreased transcriptional expression of IL-6 receptor α, an upstream activator of STAT3, both in vitro and in vivo. This may be one mechanism underlying the RORα-mediated inhibition of STAT3. Taken together, our results suggest that RORα is a regulator of the hepatic IL-6–STAT3 signaling pathway and may be a new therapeutic target for treating APR-associated inflammatory ALF.
Development and evaluation of double gene transgenic cotton lines expressing Cry toxins for protection against chewing insect pests
Authors: Siddiqui Hamid Anees , Asif Muhammad , Asad Shaheen , Naqvi Rubab Zahra , Ajaz Sobia , Umer Noroza , Anjum Naveed , Rauf Imran , Sarwar Muhammad , Arshad Muhammad , Amin Imran , Saeed Muhammad , Mukhtar Zahid , Bashir Aftab , Mansoor Shahid 
Published: August 2019

AbstractCotton is the main fiber producing crop globally, with a significant impact on the economy of Pakistan. Bt cotton expressing a Cry1Ac gene is grown over a large area in Pakistan, however, there is a major concern that bollworms may develop resistance. Here we have used a durable resistance strategy against bollworms by developing a double gene construct containing Cry1Ac and Cry2Ab (pGA482-12R) for cotton transformation. Both Cry toxin genes have been cloned in the same T-DNA borders and transferred successfully into cotton via Agrobacterium-mediated transformation. Both genes are expressed in transgenic cotton plants and is likely to help breeders in developing new cotton cultivars by incorporating these genes in cotton lines having no Bt genes or expressing Cry1Ac gene (Mon 531). Positive transgenic cotton was identified by PCR using specific primers for the amplification of both Cry1Ac and Cry2Ab genes. Cry1Ac and Cry2Ab expression was confirmed with an immunostrip test and quantified using ELISA that showed significant spatio-temporal expression of Cry2Ab ranging from 3.28 to 7.72 µg/g of the tissue leaf. Insect bioassay with army worm (Spodoptera litura) was performed to check the efficacy of NIBGE (National Institute for Biotechnology and Genetic Engineering) double gene transgenic cotton plants and up to 93% insect mortality was observed.