bluuebunny/crossref_metadata_2025_split huggingface dataset:
Review of the sustainability of food systems and transition using the Internet of Food
Authors: Holden Nicholas M. , White Eoin P. , Lange Matthew. C. , Oldfield Thomas L. 
Published: October 2018

AbstractMany current food systems are unsustainable because they cause significant resource depletion and unacceptable environmental impacts. This problem is so severe, it can be argued that the food eaten today is equivalent to a fossil resource. The transition to sustainable food systems will require many changes but of particular importance will be the harnessing of internet technology, in the form of an ‘Internet of Food’, which offers the chance to use global resources more efficiently, to stimulate rural livelihoods, to develop systems for resilience and to facilitate responsible governance by means of computation, communication, education and trade without limits of knowledge and access. A brief analysis of the evidence of resource depletion and environmental impact associated with food production and an outline of the limitations of tools like life cycle assessment, which are used to quantify the impact of food products, indicates that the ability to combine data across the whole system from farm to human will be required in order to design sustainable food systems. Developing an Internet of Food, as a precompetitive platform on which business models can be built, much like the internet as we currently know it, will require agreed vocabularies and ontologies to be able to reason and compute across the vast amounts of data that are becoming available. The ability to compute over large amounts of data will change the way the food system is analysed and understood and will permit a transition to sustainable food systems.
Working Memory Training Improves Emotion Regulation Ability
Authors: Xiu Lichao , Wu Jie , Chang Lei , Zhou Renlai 
Published: October 2018

AbstractEmotion regulation deficits are associated with various emotional disorders. Therefore, studies have attempted to improve emotion regulation ability to prevent psychopathological symptoms. Studies have revealed that working memory training—specifically attention control—can promote emotion regulation. In the present study, participants completed a running memory task in a 20-day training period. The participants’ pre- and post-test data on attention network functions and late positive potential (LPP) were assessed and analyzed. Compared with the control group, the training group’s orientation function improved markedly. In addition, LPP in relation to emotion regulation decreased during the 20-day training period. These results suggest that working memory training can improve emotion regulation ability, and the orientation function in the attention network may also contribute to this improvement.
Understanding the transfer reaction network behind the non-processive synthesis of low molecular weight levan catalyzed by Bacillus subtilis levansucrase
Authors: Raga-Carbajal Enrique , López-Munguía Agustín , Alvarez Laura , Olvera Clarita 
Published: October 2018

AbstractUnder specific reaction conditions, levansucrase from Bacillus subtilis (SacB) catalyzes the synthesis of a low molecular weight levan through the non-processive elongation of a great number of intermediates. To deepen understanding of the polymer elongation mechanism, we conducted a meticulous examination of the fructooligosaccharide profile evolution during the levan synthesis. As a result, the formation of primary and secondary intermediates series in different reaction stages was observed. The origin of the series was identified through comparison with product profiles obtained in acceptor reactions employing levanbiose, blastose, 1-kestose, 6-kestose, and neo-kestose, and supported with the isolation and NMR analyses of some relevant products, demonstrating that all of them are inherent products during levan formation from sucrose. These results allowed to establish the network of fructosyl transfer reactions involved in the non-processive levan synthesis. Overall, our results reveal how the relaxed acceptor specificity of SacB during the initial steps of the synthesis is responsible for the formation of several levan series, which constitute the final low molecular weight levan distribution.
A Pre-Clinical Large Animal Model of Sustained Liver Injury and Regeneration Stimulus
Authors: Inomata Kenta , Tajima Kazuki , Yagi Hiroshi , Higashi Hisanobu , Shimoda Hirofumi , Matsubara Kentaro , Hibi Taizo , Abe Yuta , Tsujikawa Hanako , Kitago Minoru , Shinoda Masahiro , Obara Hideaki , Itano Osamu , Soto-Gutierrez Alejandro , Kitagawa Yuko 
Published: October 2018

AbstractA feasible large animal model to evaluate regenerative medicine techniques is vital for developing clinical applications. One such appropriate model could be to use retrorsine (RS) together with partial hepatectomy (PH). Here, we have developed the first porcine model using RS and PH. RS or saline control was administered intraperitoneally to Göttingen miniature pigs twice, two weeks apart. Four weeks after the second dose, animals underwent PH. Initially, we tested different doses of RS and resection of different amounts of liver, and selected 50 mg/kg RS with 60% hepatectomy as our model for further testing. Treated animals were sacrificed 3, 10, 17 or 28 days after PH. Blood samples and resected liver were collected. Serum and liver RS content was determined by Liquid Chromatograph-tandem Mass Spectrometer. Blood analyses demonstrated liver dysfunction after PH. Liver regeneration was significantly inhibited 10 and 17 days after PH in RS-treated animals, to the extent of 20%. Histological examination indicated hepatic injury and regenerative responses after PH. Immunohistochemical staining demonstrated accumulation of Cyclin D1 and suppression of Ki-67 and PCNA in RS-treated animals. We report the development of the first large animal model of sustained liver injury with suppression of hepatic regeneration.
Ultrafast polarization control by terahertz fields via π-electron wavefunction changes in hydrogen-bonded molecular ferroelectrics
Authors: Miyamoto T. , Hata D. , Morimoto T. , Yamakawa H. , Kida N. , Terashige T. , Iwano K. , Kishida H. , Horiuchi S. , Okamoto H. 
Published: October 2018

AbstractRapid polarization control by an electric field in ferroelectrics is important to realize high-frequency modulation of light, which has potential applications in optical communications. To achieve this, a key strategy is to use an electronic part of ferroelectric polarization. A hydrogen-bonded molecular ferroelectric, croconic acid, is a good candidate, since π-electron polarization within each molecule is theoretically predicted to play a significant role in the ferroelectric-state formation, as well as the proton displacements. Here, we show that a sub-picosecond polarization modulation is possible in croconic acid using a terahertz pulse. The terahertz-pulse-pump second-harmonic-generation-probe and optical-reflectivity-probe spectroscopy reveal that the amplitude of polarization modulation reaches 10% via the electric-field-induced modifications of π-electron wavefunctions. Moreover, the measurement of electric-field-induced changes in the infrared molecular vibrational spectrum elucidates that the contribution of proton displacements to the polarization modulation is negligibly small. These results demonstrate the electronic nature of polarization in hydrogen-bonded molecular ferroelectrics. The ultrafast polarization control via π-electron systems observed in croconic acid is expected to be possible in many other hydrogen-bonded molecular ferroelectrics and utilized for future high-speed optical-modulation devices.
Deletion of exon 4 in LAMA2 is the most frequent mutation in Chinese patients with laminin α2-related muscular dystrophy
Authors: Ge Lin , Liu Aijie , Gao Kai , Du Renqian , Ding Juan , Mao Bing , Hua Ying , Zhang Xiaoli , Tan Dandan , Yang Haipo , Fu Xiaona , Fan Yanbin , Zhang Ling , Song Shujuan , Wu Jian , Zhang Feng , Jiang Yuwu , Wu Xiru , Xiong Hui 
Published: October 2018

AbstractAlthough recessive mutations in LAMA2 are already known to cause laminin α2-related muscular dystrophy, a rare neuromuscular disorder, large deletions or duplications within this gene are not well-characterized. In this study, we applied next-generation sequencing-based copy number variation profiling in 114 individuals clinically diagnosed with laminin α2-related muscular dystrophy, including 96 who harboured LAMA2 mutations and 34 who harboured intragenic rearrangements. In total, we detected 18 distinct LAMA2 copy number variations that have been reported only among Chinese, 10 of which are novel. The frequency of CNVs in the cohort was 19.3%. Deletion of exon 4 was detected in 10 alleles of eight patients, accounting for 27% of all copy number variations. These patients are Han Chinese and were found to have the same haplotype and sequence at the breakpoint junction, suggesting that exon 4 deletion is a founder mutation in Chinese Han and a mutation hotspot. Moreover, the data highlight our approach, a modified next-generation sequencing assay, as a robust and sensitive tool to detect LAMA2 variants; the assay identifies 85.7% of breakpoint junctions directly alongside sequence information. The method can be applied to clinical samples to determine causal variants underlying various Mendelian disorders.
Superiority of SpiroZin2 Versus FluoZin-3 for monitoring vesicular Zn2+ allows tracking of lysosomal Zn2+ pools
Authors: Han Yu , Goldberg Jacob M. , Lippard Stephen J. , Palmer Amy E. 
Published: October 2018

AbstractSmall-molecule fluorescent probes are powerful and ubiquitous tools for measuring the concentration and distribution of analytes in living cells. However, accurate characterization of these analytes requires rigorous evaluation of cell-to-cell heterogeneity in fluorescence intensities and intracellular distribution of probes. In this study, we perform a parallel and systematic comparison of two small-molecule fluorescent vesicular Zn2+ probes, FluoZin-3 AM and SpiroZin2, to evaluate each probe for measurement of vesicular Zn2+ pools. Our results reveal that SpiroZin2 is a specific lysosomal vesicular Zn2+ probe and affords uniform measurement of resting Zn2+ levels at the single cell level with proper calibration. In contrast, FluoZin-3 AM produces highly variable fluorescence intensities and non-specifically localizes in the cytosol and multiple vesicular compartments. We further applied SpiroZin2 to lactating mouse mammary epithelial cells and detected a transient increase of lysosomal free Zn2+ at 24-hour after lactation hormone treatment, which implies that lysosomes play a role in the regulation of Zn2+ homeostasis during lactation. This study demonstrates the need for critical characterization of small-molecule fluorescent probes to define the concentration and localization of analytes in different cell populations, and reveals SpiroZin2 to be capable of reporting diverse perturbations to lysosomal Zn2+.
The Tsunami Threat to Sydney Harbour, Australia: Modelling potential and historic events
Authors: Wilson Kaya M. , Allen Stewart C. R. , Power Hannah E. 
Published: October 2018

AbstractTsunami modelling of potential and historic events in Australia’s Sydney Harbour quantifies the potentially damaging impacts of an earthquake generated tsunami. As a drowned river valley estuary exposed to distant source zones, these impacts are predominantly high current speeds (>2 m/s), wave amplification and rapid changes in water level. Significant land inundation only occurs for scenarios modelled with the largest waves (9.0 MW source). The degree of exposure to the open ocean and the geomorphology of locations within the Harbour determine the relative level of these impacts. Narrow, shallow channels, even those sheltered from the open ocean, create a bottleneck effect and experience the highest relative current speeds as well as elevated water levels. The largest maximum water levels (>8 m) occur in exposed, funnel-shaped bays and wave amplification is greatest at locations exposed to the open ocean: >7 times deep water wave heights for 9.0 MW source waves. Upstream attenuation rates of runup and maximum water level show a linear correlation with wave height parameters at the 100 m depth contour and may provide some predictive capabilities for potential tsunami impacts at analogous locations. In the event of a tsunami in Sydney Harbour, impacts may threaten marine traffic and infrastructure.
Carbon stocks and accumulation rates in Red Sea seagrass meadows
Authors: Serrano Oscar , Almahasheer Hanan , Duarte Carlos M. , Irigoien Xabier 
Published: October 2018

AbstractSeagrasses play an important role in climate change mitigation and adaptation, acting as natural CO2sinks and buffering the impacts of rising sea level. However, global estimates of organic carbon (Corg) stocks, accumulation rates and seafloor elevation rates in seagrasses are limited to a few regions, thus potentially biasing global estimates. Here we assessed the extent of soil Corgstocks and accumulation rates in seagrass meadows (Thalassia hemprichii,Enhalus acoroides,Halophila stipulacea,Thalassodendrum ciliatumandHalodule uninervis) from Saudi Arabia. We estimated that seagrasses store 3.4 ± 0.3 kg Corgm−2in 1 m-thick soil deposits, accumulated at 6.8 ± 1.7 g Corgm−2yr−1over the last 500 to 2,000 years. The extreme conditions in the Red Sea, such as nutrient limitation reducing seagrass growth rates and high temperature increasing soil respiration rates, may explain their relative low Corgstorage compared to temperate meadows. Differences in soil Corgstorage among habitats (i.e. location and species composition) are mainly related to the contribution of seagrass detritus to the soil Corgpool, fluxes of Corgfrom adjacent mangrove and tidal marsh ecosystems into seagrass meadows, and the amount of fine sediment particles. Seagrasses sequester annually around 0.8% of CO2emissions from fossil-fuels by Saudi Arabia, while buffering the impacts of sea level rise. This study contributes data from understudied regions to a growing dataset on seagrass carbon stocks and sequestration rates and further evidences that even small seagrass species store Corgin coastal areas.
Microclimate exerts greater control over litter decomposition and enzyme activity than litter quality in an alpine forest-tundra ecotone
Authors: Chen Yamei , Liu Yang , Zhang Jian , Yang Wanqin , He Runlian , Deng Changchun 
Published: October 2018

AbstractPlant litter decomposition is an important biogeochemical process in terrestrial ecosystems. Although climate and substrate quality controls over litter decomposition are reasonably well understood, their impacts on lignocellulose degradation and lignocellulolytic enzymes remain elusive. Here, the decomposition of three leaf litters derived from Salix paraplesia (SP), Deyeuxia scabrescens (DS), and Ajuga ovalifolia (AO), was studied across an alpine forest-tundra ecotone during one snow-covered season with the objective of distinguishing between the effects of microclimate and litter quality on litter decomposition rates and lignocellulolytic enzymes. The results showed that both microclimate and litter quality affected lignocellulose degradation rates and lignocellulolytic enzyme activities; however, microclimate factors had the greater effects. Interestingly, freeze-thaw cycles and moisture were the predominant factors explaining the variations in decomposition rate and enzyme activities. Higher cellulose degradation rates were associated with higher cellulose concentrations. Cellulolytic enzymes had a greater effect on litter decomposition than did ligninolytic enzymes at the early decomposition stage. Litter decomposition and enzyme activities should be given more attention under global climate change, as the direction and magnitude of changes in microclimate factors and litter quality could strongly influence the nutrient cycling and energy fluxes of alpine ecosystems.
Positive effects of the catastrophic Hurricane Patricia on insect communities
Authors: Novais Samuel , Macedo-Reis Luiz Eduardo , Cristobal-Peréz E. Jacob , Sánchez-Montoya Gumersindo , Janda Milan , Neves Frederico , Quesada Mauricio 
Published: October 2018

AbstractHighly seasonal conditions of tropical dry forests determine the temporal patterns of insect abundance. However, density-independent factors such as natural disturbances can abruptly change environmental conditions, affecting insect populations. We address the effects of the Hurricane Patricia (category 5) on species density and abundance of three feeding guilds of herbivorous insects (sap-sucking, folivorous beetles and xylophagous) and predatory beetles associated to the canopy of a tropical dry forest. Hurricane Patricia has been the strongest tropical hurricane ever reported in the Western Hemisphere. Herbivorous insects (sap-sucking and xylophagous) and predatory beetles increased in species density and abundance in the following months after the hurricane, compared to samples before it. The positive response of sap-sucking insects to Hurricane Patricia was probably related to an increase in the availability of new shoots and leaf meristems after the natural coppicing by the hurricane, while xylophagous guild seems to have been positively affected by the increase in the amount and diversity of deadwood resources. The positive response of predatory beetles may be the result of a bottom-up effect due to a greater availability of arthropod preys after the hurricane. We demonstrated that catastrophic hurricane disturbances could be important events that temporarily increase the species density and abundance of insects in tropical dry forests.
Verbal instructions override the meaning of facial expressions
Authors: Bublatzky Florian , Guerra Pedro , Alpers Georg W. 
Published: October 2018

AbstractPsychological research has long acknowledged that facial expressions can implicitly trigger affective psychophysiological responses. However, whether verbal information can alter the meaning of facial emotions and corresponding response patterns has not been tested. This study examined emotional facial expressions as cues for instructed threat-of-shock or safety, with a focus on defensive responding. In addition, reversal instructions were introduced to test the impact of explicit safety instructions on fear extinction. Forty participants were instructed that they would receive unpleasant electric shocks, for instance, when viewing happy but not angry faces. In a second block, instructions were reversed (e.g., now angry faces cued shock). Happy, neutral, and angry faces were repeatedly presented, and auditory startle probes were delivered in half of the trials. The defensive startle reflex was potentiated for threat compared to safety cues. Importantly, this effect occurred regardless of whether threat was cued by happy or angry expressions. Although the typical pattern of response habituation was observed, defense activation to newly instructed threat cues remained significantly enhanced in the second part of the experiment, and it was more pronounced in more socially anxious participants. Thus, anxious individuals did not exhibit more pronounced defense activation compared to less anxious participants, but their defense activation was more persistent.
Intranasal delivery of a Fas-blocking peptide attenuates Fas-mediated apoptosis in brain ischemia
Authors: Ullah Irfan , Chung Kunho , Oh Jungju , Beloor Jagadish , Bae Sumin , Lee Sangah Clara , Lee Minhyung , Kumar Priti , Lee Sang-Kyung 
Published: October 2018

AbstractIschemic stroke-induced neuronal cell death results in the permanent disabling of brain function. Apoptotic mechanisms are thought to play a prominent role in neuronal injury and ample evidence implicates Fas signaling in mediating cell death. In this study, we describe the neuroprotective effects of a Fas-blocking peptide (FBP) that by obstructing Fas signaling in cerebral ischemia inhibits apoptosis. Using an intranasal administration route in a rat model of focal cerebral ischemia, we demonstrate that nose-to-brain delivery of FBP after middle cerebral artery occlusion (MCAO) surgery results in the delivery and retention of FBP in Fas-expressing ischemic areas of the brain. A single intranasal administration of 2 mg/kg FBP resulted in significantly reduced neuronal cell death by inhibiting Fas-mediated apoptosis leading to decreased infarct volumes, reduced neurologic deficit scores and recovery from cerebral ischemia. Intranasally delivered FBP might be a promising strategy for the treatment of cerebral ischemic stroke.
Promiscuous attraction of ligands within the ATP binding site of RyR2 promotes diverse gating behaviour
Authors: Lindsay Chris , Sitsapesan Mano , Chan Wei Mun , Venturi Elisa , Welch William , Musgaard Maria , Sitsapesan Rebecca 
Published: October 2018

AbstractATP is an essential constitutive regulator of cardiac ryanodine receptors (RyR2), enabling small changes in cytosolic Ca2+ to trigger large changes in channel activity. With recent landmark determinations of the full structures of RyR1 (skeletal isoform) and RyR2 using cryo-EM, and identification of the RyR1 ATP binding site, we have taken the opportunity to model the binding of fragments of ATP into RyR2 in order to investigate how the structure of the ATP site dictates the functional responses of ligands attracted there. RyR2 channel gating was assessed under voltage-clamp conditions and by [3H]ryanodine binding studies. We show that even the triphosphate (PPPi) moiety alone was capable of activating RyR2 but produced two distinct effects (activation or irreversible inactivation) that we suggest correspond to two preferred binding locations within the ATP site. Combinations of complementary fragments of ATP (Pi + ADP or PPi + AMP) could not reproduce the effects of ATP, however, the presence of adenosine prevented the inactivating PPPi effects, allowing activation similar to that of ATP. RyR2 appears to accommodate diverse types of molecules, including PPPi, deep within the ATP binding site. The most effective ligands, however, have at least three phosphate groups that are guided into place by a nucleoside.
Genetic variations in TAS2R3 and TAS2R4 bitterness receptors modify papillary carcinoma risk and thyroid function in Korean females
Authors: Choi Jeong-Hwa , Lee Jeonghee , Yang Sarah , Lee Eun Kyung , Hwangbo Yul , Kim Jeongseon 
Published: October 2018

AbstractType 2 taste receptors (T2Rs, TAS2Rs) mediate bitterness perception and are involved in diverse defence mechanisms in extraoral tissues. The thyrocyte-expressed T2Rs control thyroid hormone production, and this regulatory role may be associated with susceptibility to thyroid diseases. This study examined whether the variations in TAS2Rs modify the risk of papillary thyroid carcinoma (PTC) and whether such T2R-related PTC risk is associated with genetically modified thyroid function. We conducted a case-control study with 763 Korean females, including 250 PTC cases. Seventy-three single-nucleotide polymorphisms in 13 TAS2R genes and the pre-diagnosis levels of 4 thyroid-related functional markers [total triiodothyronine (TT3), free thyroxine, thyroid-stimulating hormone and thyroglobulin] were analysed. Individuals with TAS2R3/4 CC haplotype (rs2270009 and rs2234001) were at a lower risk for PTC than those with the remaining haplotypes (odds ratio = 0.59, 95% confidence interval: 0.36–0.97). Furthermore, TT3 levels were significantly reduced for TAS2R3/4 CC haplotype carriers compared with other haplotype carriers (p = 0.005). No other genetic variants exhibited critical associations with the PTC phenotype and biomarkers. In summary, genetic variations in T2R3/4 bitterness receptors may modify the PTC risk, and the genetically modified thyroid hormone level by those variations may be linked with the PTC-T2Rs association.