bluuebunny/crossref_metadata_2025_split huggingface dataset:
A facile isoelectric focusing of myoglobin and hemoglobin used as markers for screening of chicken meat quality in China
Authors: Saud Shah , Li Guoqing , Sun Yingjie , Khan Muhammad Idrees , Ur Rehman Ashfaq , Uzzaman Asad , Liu Weiwen , Ding Chan , Xiao Hua , Wang Yuxing , Cao Chengxi 
Published: June 2019

AbstractA novel analytical protocol was developed for general quality screening of chicken meat based on IEF and protein extraction. To demonstrate the developed protocol, 24 chickens were divided into three groups; each had eight chickens. The chickens in Group 1 were slaughtered by exsanguination, Group 2 asphyxiated in water, and that in Group 3 were infected by new castle disease virus. Proteins were extracted from the meat samples by using pure water as an extractant, separated by IEF, verified by western blot, and quantified via imaging analysis. The relevant experiments demonstrated that two myoglobin (Mb) bands were detected at pI 6.8 and 7.04 for all samples of Groups 1, 2, and 3, but there were additional hemoglobin (Hb) bands at pI 7.09 and 7.13 (P < 0.05) for the samples of Groups 2 and 3. The results implied that Hb bands might be a potential biomarker for the screening of chicken meat quality. The RSD values of two Mb bands (pI 6.8 and 7.04) in Group 1 were respectively 4.08 and 3.63%, the ones of two Hb bands (pI 7.09 and 7.13) in Group 2 were 3.66 and 2.10%, and those in Group 3 were 2.17% and 2.77%, respectively. All the RSD values indicated high stability and reliability of the developed protocol. Additionally, the protocol had a direct readout of protein bands in IEF without staining. However, it was time‐consuming and had high cost. Even so, the relevant general method and finding have potential for screening of chicken meat quality.
A data‐driven approach to detecting change points in linear regression models
Authors: Lyubchich Vyacheslav , Lebedeva Tatiana V. , Testa Jeremy M. 
Published: June 2019

AbstractChange points appear in various types of environmental data—from univariate time series to multivariate data structures—and need to be accounted for in proper analysis and inference. The analysis of change points is challenging when no exact information about their number and locations is available, and statistical tests developed under such conditions often have low power identifying the change points. This paper provides a powerful, data‐driven procedure for detecting at‐most‐m change points in linear regression models by adapting a sieve bootstrap approach for a modified cumulative sum statistic. The new procedure does not assume a particular dependence structure nor a particular distribution of regression residuals. It employs a data‐driven selection of the order of an autoregressive model and a robust estimation of the model coefficients. Our simulation studies show a competitive performance of the new bootstrap‐based procedure compared with its asymptotic counterpart. We apply the new testing procedure to address an important environmental problem in Chesapeake Bay—severe oxygen depletion—and detect two change points in the relationship between the volume of low‐oxygen waters and nutrient inputs to the bay during 1985–2017.
Breakage features of coal treated by cyclic single pulse electrical disintegration
Authors: Zhang Xiangliang , Lin Baiquan , Li Yanjun , Zhu Chuanjie , Li Yong 
Published: June 2019

AbstractHigh‐voltage electrical pulse (HVEP) technology, a potential method for degassing coal seam in the future, has made great progress in field application. However, the previous studies mainly concentrated on the coal‐crushing effect of the action of single pulse, ignoring the influence of cyclic single pulse on the fracture structure of coal. In this paper, Huaibei anthracite coal and Inner Mongolia bituminous coal were taken as the experimental objects to study the effect of cyclic single pulse on the fracture structure of coal. The results show that the uneven distribution of electric field in the coal causes the crack to break along the interface between the mineral and the coal. Besides, the characteristics of cracks and functional groups of coal before and after electrical breakdown were investigated through scanning electron microscope and Fourier transform infrared spectroscopy. The results suggest that the relative contents of oxygen‐containing functional groups are reduced after electrical breakdown, which promotes the gas desorption from the surfaces of coal samples. Moreover, changes in CH4 adsorption capacity of HVEP‐treated coal were studied through a high‐pressure adsorption instrument, with the adsorption temperature set from 40 to 120°C. The results show that the adsorption amount of electrically broken coal is smaller than that of raw coal, which proves that temperature and current exert similar influences on the adsorption capacity of coal sample. Additionally, current waveforms indicate that the peak current increases with the number of cycle, whereas the breakdown time decreases with it. However, the peak current and breakdown time will eventually become stable as the number of cycles grows, demonstrating that electrical properties of coal are changed in the process of breakdown, which thus affects the next discharge.
The origin of smectite in the soil of the Kruger 2 archaeological site, Brompton (Québec), Canada
Authors: Courchesne François , Chapdelaine Claude , Munro Lara 
Published: June 2019

AbstractKruger 2 is unique among Late Paleoindian sites of eastern Canada because of the presence of a potential hearth (feature #1) characterized by a concentration of blackened fire‐cracked rock and burnt bone embedded in a thick Ae horizon. Comparative mineralogical analysis (X‐ray diffraction) of Ae samples collected inside and outside the feature reveal the absence of calcite and apatite, two minerals commonly found in ashes, and the presence of smectite in the Ae inside feature #1. Smectite genesis is attributed to the weathering of mica and chlorite under geochemical conditions (high base cations, Si and pH; low Al) that are unique in time and space. We hypothesize that these conditions were created by the dissolution, 10,000 years ago, of a layer of hearth ashes resting on an incipient soil. Results confirm the intense weathering of mica and chlorite. We also show that ash dissolution could generate the conditions for smectite formation in the presence of altered mica and chlorite, allowing the development of a chronology explaining this finding. The data largely support our hypothesis and constitute a strong basis for future investigations on the links between hearth ash weathering and smectite genesis in acidic soils.
Methods for public health economic evaluation: A Delphi survey of decision makers in English and Welsh local government
Authors: Frew Emma , Breheny Katie 
Published: June 2019

AbstractStandard reference case methods recommended for health technology appraisals do not translate well to a public health setting. This paper reports on a Delphi survey designed to elicit views of public health decision makers in England and Wales, about different methodological elements of economic evaluation. This is important as methods should align with the objective function of decision makers. The Delphi survey comprised two rounds, with round 1 allowing open‐ended recommendations in addition to 5‐point Likert scale responses. The final survey comprised 36 questions, and levels and strength of agreement were assessed using median values and mean absolute deviation of the median. The Delphi panel (n = 66) achieved high levels of agreement for costs, health, well‐being, and productivity impact to be important elements within an economic evaluation. The panel agreed that evaluations should be relevant to the local context and include costs and consequences over a lifetime horizon. There was a call for the transparent reporting of costs and effects for different population subgroups, and for different sectors. Overall, the panel revealed a preference for a flexible approach, understanding that economic evidence fits within a dynamic process of decision making. These results provide empirical evidence to inform guidelines for public health economic evaluation.
Hsp105α suppresses Adriamycin‐induced cell death via nuclear localization signal‐dependent nuclear accumulation
Authors: Yamane Teppei , Saito Youhei , Teshima Hiroko , Hagino Mari , Kakihana Ayana , Sato Saki , Shimada Masashi , Kato Yoshiho , Kuga Takahisa , Yamagishi Nobuyuki , Nakayama Yuji 
Published: June 2019

AbstractHeat shock protein 105 (Hsp105) is a molecular chaperone, and the isoforms Hsp105α and Hsp105β exhibit distinct functions with different subcellular localizations. Hsp105β localizes in the nucleus and induces the expression of the major heat shock protein Hsp70, whereas cytoplasmic Hsp105α is less effective in inducing Hsp70 expression. Hsp105 shuttles between the cytoplasm and the nucleus; the subcellular localization is governed by the relative activities of the nuclear localization signal (NLS) and nuclear export signal (NES). Here, we show that nuclear accumulation of Hsp105α but not Hsp105β is involved in Adriamycin (ADR) sensitivity. Knockdown of Hsp105α induces cell death at low ADR concentration, at which ADR is less effective in inducing cell death in the presence of Hsp105α. Of note, Hsp105 is localized in the nucleus under these conditions, even though Hsp105β is not expressed, indicating that Hsp105α accumulates in the nucleus in response to ADR treatment. The exogenously expressed Hsp105α but not its NLS mutant localizes in the nucleus of ADR‐treated cells. In addition, the expression level of the nuclear export protein chromosomal maintenance 1 (CRM1) was decreased by ADR treatment of cells, and CRM1 knockdown caused nuclear accumulation of Hsp105α both in the presence and absence of ADR. These results indicating that Hsp105α accumulates in the nucleus in a manner dependent on the NLS activity via the suppression of nuclear export. Our findings suggest a role of nuclear Hsp105α in the sensitivity against DNA‐damaging agents in tumor cells.
Green synthesized‐zinc oxide nanoparticles, the strong apoptosis inducer as an exclusive antitumor agent in murine breast tumor model and human breast cancer cell lines (MCF7)
Authors: Mahdizadeh Roya , Homayouni‐Tabrizi Masoud , Neamati Ali , Seyedi Seyed Mohammad Reza , Tavakkol Afshari Haleh Sadat 
Published: June 2019

AbstractPurposeIn the present study, we aimed to synthesize and investigate the impact of zinc oxide nanoparticle (ZnONPs) on both human and murine breast cancer cell lines and define their untoxic concentrations (IC50) to clarify their apoptotic properties and introduce them as the anticancer agents.Materials and MethodsThe in vitro study was initiated by ZnONPs green synthesizing process applying the Cucumis melo inodorus rough shell extract, and verified by the transmission electron microscope, scanning electron microscopy, Fourier‐transform infrared spectroscopy, and X‐ray diffraction analysis. In following, the human (Michigan Cancer Foundation‐7 [MCF7]) and murine (TUBO) breast cancer cell lines were cultured for taking the time and dose‐dependent treatment planes by ZnONPs. Also, MCF7 cell cultures were treated by three different doses of ZnoNPs (8, 4, and 2 µg/mL) separately and prepared for genes expression (Cas‐3 and Cas‐8) analysis using real‐time quantitative PCR method. The in vivo initiated by providing the 39 murine breast cancer models, then they were injected intraperitoneally with different doses of ZnONPs (75, 50, and 25 mg/kg) treatments. Then their collected biopsies were stained by hematoxylin and eosin to evaluate their breast cancer tissue morphology and compare with Tamoxifen anticancer properties.ResultsThe in vitro study results demonstrate a significant correlation among the expression of Cas‐3 and Cas‐8 genes with increasing ZnONPs concentrations. The results of 3‐(4,5‐dimethylthiazole‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assays for the treated cancer cell lines (MCF7 and TUBO) detected a significant negative correlation among the ZnONPs concentrations and the viability of the cells.ConclusionUnlike the majority of resent studies, we found the ZnONPs as a powerful apoptosis inducer in the human cell line (MCF7) and murine (TUBO cell line and cancer model).
Sirt3 modulate renal ischemia‐reperfusion injury through enhancing mitochondrial fusion and activating the ERK‐OPA1 signaling pathway
Authors: Wang Qiang , Xu Junnan , Li Xiaoli , Liu Zhijia , Han Yong , Xu Xiaoguang , Li Xiubin , Tang Yuzhe , Liu Yubao , Yu Tao , Li Xiang 
Published: June 2019

AbstractMitochondrial fusion is linked to heart and liver ischemia‐reperfusion (IR) insult. Unfortunately, there is no report to elucidate the detailed influence of mitochondrial fusion in renal IR injury. This study principally investigated the mechanism by which mitochondrial fusion protected kidney against IR injury. Our results indicated that sirtuin 3 (Sirt3) was inhibited after renal IR injury in vivo and in vitro. Overexpression of Sirt3 improved kidney function, modulated oxidative injury, repressed inflammatory damage, and reduced tubular epithelial cell apoptosis. The molecular investigation found that Sirt3 overexpression attenuated IR‐induced mitochondrial damage in renal tubular epithelial cells, as evidenced by decreased reactive oxygen species production, increased antioxidants sustained mitochondrial membrane potential, and inactivated mitochondria‐initiated death signaling. In addition, our information also illuminated that Sirt3 maintained mitochondrial homeostasis against IR injury by enhancing optic atrophy 1 (OPA1)‐triggered fusion of mitochondrion. Inhibition of OPA1‐induced fusion repressed Sirt3 overexpression‐induced kidney protection, leading to mitochondrial dysfunction. Further, our study illustrated that OPA1‐induced fusion could be affected through ERK; inhibition of ERK abolished the regulatory impacts of Sirt3 on OPA1 expression and mitochondrial fusion, leading to mitochondrial damage and tubular epithelial cell apoptosis. Altogether, our results suggest that renal IR injury is closely associated with Sirt3 downregulation and mitochondrial fusion inhibition. Regaining Sirt3 and/or activating mitochondrial fission by modifying the ERK‐OPA1 cascade may represent new therapeutic modalities for renal IR injury.
Targeting IL‐34 in inflammatory autoimmune diseases
Authors: Xu Wang‐Dong , Huang An‐Fang , Fu Lu , Liu Xiao‐Yan , Su Lin‐Chong 
Published: June 2019

AbstractInterleukin‐34 (IL‐34) shares a common receptor with macrophage colony‐stimulating factor (M‐CSF), and can bind to CSF‐1R, induces lymphocytes differentiation, proliferation, and regulates the synthesis of inflammatory components. Recent findings reported aberrant expression of IL‐34 in several autoimmune disorders, such as lupus, arthritis, systemic sclerosis, inflammatory bowel diseases. The functional analysis further demonstrated that IL‐34 may perform significantly in these inflammatory autoimmune disorders. IL‐34 might consider as a biomarker for these diseases. I hope this collection of the findings in this review will improve knowledge of the role of IL‐34, and targeting IL‐34 may give the potential for these autoimmune diseases.
Disease Progression Modeling Analysis of the Change of Bone Mineral Density by Postoperative Hormone Therapies in Postmenopausal Patients With Early Breast Cancer
Authors: Yoon SeokKyu , Bae Kyun‐Seop , Cho Yong‐Soon , Han Sunpil , Kim Hyungsub , Lim Hyeong‐Seok 
Published: June 2019

AbstractThe osteoporosis incidence in postmenopausal patients on aromatase inhibitors (AI) is much higher than in those on tamoxifen, and adverse effects other than musculoskeletal disorders are less on AI than on tamoxifen. In this study we performed disease‐progression modeling in postmenopausal patients with early breast cancer who had received 5 years of postoperative hormone therapy. Clinical data from postmenopausal patients who had received postoperative hormonal therapy and met the predefined selection criteria were retrospectively collected in an anonymized way. Disease‐progression modeling and simulations were performed using NONMEM version 7.42. A first‐order deterioration model with a combination of a symptomatic model (when a drug effect provides a transient bad effect by offsetting the severity of the disease) and a disease‐modifying model (when a drug affects the disease progression rate) was used. Vitamin D supplementation was found to have a disease‐modifying effect in osteoporosis, whereas AI decreased the bone mineral density by a t score of –0.21. However, after stopping the AI, the estrogen level reverted to normal, thereby reexercising protective effects against bone loss. In the simulation the probability of osteoporosis increased by 10% in the AI group compared with the other groups (tamoxifen, no‐treatment group) during the medication period. Tamoxifen showed no significant effects in the final model.
Solar‐powered BDD‐electrolysis remediation of soil washing fluid spiked with diesel
Authors: de Melo Henrique João Miller , de Andrade Déborah Cordeiro , Barros Neto Eduardo L , da Silva Djalma Ribeiro , dos Santos Elisama Vieira 
Published: June 2019

AbstractBACKGROUNDThe remediation of a soil spiked with diesel using Unitol L90 and sodium dodecyl sulfate (SDS) in a soil washing process was evaluated. The washing process was followed by an anodic oxidation based on boron doped‐diamond electrolysis (BDD‐electrolysis) to treat the washing liquid, using a solar photovoltaic (PV)‐battery system as a direct electrical power source.RESULTSThe results demonstrate that high concentrations of Unitol L 90 and SDS are required for the surfactant‐aided soil‐washing (SASW) to efficiently extract diesel from the soil. However, Unitol L 90 strongly interacts with the soil due to the adsorption process. The main characteristics of the effluents produced during this soil remediation was low conductivity. Therefore, the addition of 0.05 mol L–1 sodium sulfate (Na2SO4) as an electrolyte, at current density of 30 mA cm−2, was investigated. BDD‐electrolysis demonstrated that total organic carbon (TOC) and total petroleum hydrocarbon (TPH) were removed more effectively using SDS. The key of the treatment is associated to the electrogeneration of S2O82− due to the contribution of the surfactant composition and the addition of 0.05 mol L–1 Na2SO4. Additionally, an average solar irradiation intensity and integration of PV cells produced more than 10 A current and continuous electricity (∼27 V), which was constantly supplied to the electrolytic reactor at all times through a direct current generator.CONCLUSIONSFinally, based on the achieved results, BDD‐electrolysis is an efficient technology approach to remove hydrocarbons from the effluent produced during the soil washing process. © 2019 Society of Chemical Industry
Taxonomic significance of foliar epidermal morphology in Lamiaceae from Pakistan
Authors: Gul Saba , Ahmad Mushtaq , Zafar Muhammad , Bahadur Saraj , Celep Ferhat , Sultana Shazia , Begum Najla , Hanif Uzma , Zaman Wajid , Shuaib Muhammad , Ayaz Asma 
Published: June 2019

AbstractFoliar micromorphological features are useful to elucidate the taxonomy and systematics of the Lamiaceae species. Leaf epidermal morphology using scanning electron microscopy and light microscopy of 22 Lamiaceae species from 15 genera have been investigated with an aim to solve its taxonomic problem in the correct identification. Various foliar micromorphological features were observed to explain their importance in resolving the correct identification of Lamiaceae taxa. Two main types of trichomes were observed; glandular trichomes (GTs) and nonglandular trichomes (NGTs). GTs were further divided into seven subtypes including the capitate, subsessile capitate, sessile capitate, sunken, barrel, peltate, and clavate. Similarly, NGTs were also divided into simple unicellular and multicellular including conical, falcate, cylindrical, dendrite, papillose, and short hook shape. Quantitative measurement includes the length and width of the trichomes, stomatal complex, epidermal cells, stomata, and trichomes index. Based on the foliar micromorphological characters, a taxonomic key was developed to delimit and correctly identify studied taxa. Further molecular, other anatomical and phylogenetic studies are recommended to strengthen the systematics of Lamiaceae.
The microfoundations of an operational capability in digital manufacturing
Authors: Roscoe Samuel , Cousins Paul D. , Handfield Robert 
Published: June 2019

AbstractThis article seeks insights into how individuals, processes, and structures interact to form the microfoundations of an operational capability in digital manufacturing. Using a knowledge‐based theory lens, we develop an empirical framework that explains how structures and processes encourage individuals to interact and share knowledge, and through these interactions, operating routines and operational capabilities emerge. The model is further refined using data collected from 40 interviews, steering committee meetings and participant observations at a high technology aerospace company. We find that discrete technologies, used in one component or subassembly, can be developed within authority‐based hierarchies using rigid new product development processes. We also find that whole system technologies that affect multiple aspects of the final product require flexible processes and consensus‐based hierarchical structures. Consensus‐based structures include centers of competence, which provide individuals the freedom to “learn through failure” and develop flexible ad hoc problem solving processes. Such flexible processes encourage individuals to learn from their mistakes and share new knowledge on a repetitive basis, leading to the emergence of operating routines. The paper contributes to the knowledge‐based view by empirically demonstrating how different types of new technology development programs, be they for discrete or whole system technologies, may benefit from different configurations of flexible/rigid processes and authority‐based/consensus‐based structures.