bluuebunny/crossref_metadata_2025_split huggingface dataset:
High prevalence of hyperlipidaemia in patients with AV re-entry tachycardia and AV nodal re-entry tachycardia
Authors: Zeljković Ivan , Đula Kristijan , Babacanli Alen , Kruljac Ivan , Mustapić Vito , Brkljačić Diana Delić , Bulj Nikola , Radeljić Vjekoslav , Manola Šime , Pavlović Nikola 
Published: August 2019

AbstractDiet rich in lipids and hyperlipidaemia increases incidence of atrial premature beats and all supraventricular arrhythmias. The aim of the study was to investigate the prevalence of hyperlipidaemia in patients with AV re-entry tachycardia (AVRT) and AV nodal re-entry tachycardia (AVNRT). We conducted a retrospective, cross-sectional, case-control study that included all consecutive patients for whom AVRT or AVNRT was confirmed during electrophysiology study. Age and gender-matched patients admitted to hospital or outpatient clinic for various reasons were randomly included and served as a control group. Hyperlipidaemia was defined according to 2016 European Society of Cardiology guidelines. A total of 1448 subjects were included: 725 patients with AVRT/AVNRT and 723 controls. AVRT/AVNRT patients had high hyperlipidaemia prevalence, which was significantly higher when compared to the control group (50.1 vs. 35.8%, p < 0.001). AVRT patients, with median age of 37.5 years, had hyperlipidaemia prevalence of 45.7%. In a multivariate analysis, hyperlipidaemia was independently associated with AVRT/AVNRT (OR 2.128, p < 0.001), both with AVNRT (OR 1.878, p < 0.001) and AVRT (OR 2.786, p < 0.001). Hypercholesterolemia was significantly more prevalent in patients with AVNRT and AVRT, while this was not the case for hypertriglyceridemia. There were no differences between the AVRT and AVNRT patients regarding hyperlipidaemia prevalence (51.9 vs. 45.7%, p = 0.801), even though AVRT patients were significantly younger (37.5 vs. 48.5, p < 0.001). In conclusion, this is the first study that investigated hyperlipidaemia prevalence in patients with AVRT or AVNRT. AVRT/AVNRT patients had higher prevalence of hyperlipidaemia and higher total and LDL cholesterol levels.
Association between Clinical and Laboratory Markers and 5-year Mortality among Patients with Stroke
Authors: Chen Chien-Min , Lee Meng , Yang Yao-Hsu , Huang Shih-Shin , Lin Chu-Hsu 
Published: August 2019

AbstractFactors influencing long-term stroke mortality have not been comprehensively investigated. This study aimed to identify the baseline clinical, laboratory, demographic/socioeconomic, and hospital factors influencing 5-year mortality in patients with first stroke. Total 3,956 patients with first-stroke hospitalization from 2004 to 2008 were connected to the longitudinal National Health Insurance Research Database. Post-admission baseline data that significantly increased 5-year mortality were red cell distribution width (RDW) >0.145 (adjusted hazard ratio [aHR] = 1.71), hemoglobin <120 g/L (aHR = 1.25), blood sugar <3.89 mmol/L (70 mg/dL)(aHR = 2.57), serum creatinine >112.27 μmol/L (aHR = 1.76), serum sodium <134 mmol/L (aHR = 1.73), body mass index (BMI) < 18.5 kg/m2(aHR = 1.33), Glasgow Coma Scale <15 (aHR = 1.43), Stroke Severity Index ≥20 (aHR = 3.92), Charlson–Deyo Comorbidity Index ≥3 (aHR = 4.21), no rehabilitation (aHR = 1.86), and age ≥65 years (aHR = 2.25). Hemoglobin, RDW, blood sugar, serum creatinine and sodium, BMI, consciousness, stroke severity, comorbidity, rehabilitation, and age were associated with 5-year mortality in patients with first stroke.
Elucidation of Galactomannan Biosynthesis Pathway Genes through Transcriptome Sequencing of Seeds Collected at Different Developmental Stages of Commercially Important Indian Varieties of Cluster Bean (Cyamopsis tetragonoloba L.)
Authors: Chaudhury Ashok , Kaila Tanvi , Gaikwad Kishor 
Published: August 2019

AbstractCyamopsis tetragonoloba (L) endosperm predominantly contains guar gum a polysaccharide, which has tremendous industrial applications in food, textile, paper, oil drilling and water treatment. In order to understand the genes controlling galactomannan biosynthesis, mRNA was isolated from seeds collected at different developmental stages; young pods, mature pods and young leaf from two guar varieties, HG365 and HG870 and subjected to Illumina sequencing. De novo assembly of fourteen individual read files from two varieties of guar representing seven developmental stages gave a total of 1,13,607 contigs with an N50 of 1,244 bases. Annotation of assemblies with GO mapping revealed three levels of distribution, namely, Biological Processes, Molecular Functions and Cellular Components. GO studies identified major genes involved in galactomannan biosynthesis: Cellulose synthase D1 (CS D1) and GAUT-like gene families. Among the polysaccharide biosynthetic process (GO:0000271) genes the transcript abundance for CS was found to be predominantly more in leaf samples, whereas, the transcript abundance for GAUT-like steadily increased from 65% to 90% and above from stage1 to stage5 indicating accumulation of galactomannan in developing seeds; and validated by qRT-PCR analysis. Galactomannan quantification by HPLC showed HG365 (12.98–20.66%) and HG870 (7.035–41.2%) gradually increasing from stage1 to stage 5 (10–50 DAA) and highest accumulation occurred in mature and dry seeds with 3.8 to 7.1 fold increase, respectively. This is the first report of transcriptome sequencing and complete profiling of guar seeds at different developmental stages, young pods, mature pods and young leaf material from two commercially important Indian varieties and elucidation of galactomannan biosynthesis pathway. It is envisaged that the data presented herein will be very useful for improvement of guar through biotechnological interventions in future.
Electromagnetic fields alter the motility of metastatic breast cancer cells
Authors: Garg Ayush Arpit , Jones Travis H. , Moss Sarah M. , Mishra Sanjay , Kaul Kirti , Ahirwar Dinesh K. , Ferree Jessica , Kumar Prabhat , Subramaniam Deepa , Ganju Ramesh K. , Subramaniam Vish V. , Song Jonathan W. 
Published: August 2019

AbstractInteractions between cells and their environment influence key physiologic processes such as their propensity to migrate. However, directed migration controlled by extrinsically applied electrical signals is poorly understood. Using a novel microfluidic platform, we found that metastatic breast cancer cells sense and respond to the net direction of weak (∼100 µV cm−1), asymmetric, non-contact induced Electric Fields (iEFs). iEFs inhibited EGFR (Epidermal Growth Factor Receptor) activation, prevented formation of actin-rich filopodia, and hindered the motility of EGF-treated breast cancer cells. The directional effects of iEFs were nullified by inhibition of Akt phosphorylation. Moreover, iEFs in combination with Akt inhibitor reduced EGF-promoted motility below the level of untreated controls. These results represent a step towards isolating the coupling mechanism between cell motility and iEFs, provide valuable insights into how iEFs target multiple diverging cancer cell signaling mechanisms, and demonstrate that electrical signals are a fundamental regulator of cancer cell migration.
Autoactivation and calpain-1-mediated shedding of hepsin in human hepatoma cells
Authors: Wang Lina , Zhang Ce , Sun Shijin , Chen Yue , Hu Yae , Wang Hao , Liu Meng , Dong Ningzheng , Wu Qingyu 
Published: August 2019

Abstract
Hepsin is a transmembrane serine protease implicated in many biological processes, including hepatocyte growth, urinary protein secretion, auditory nerve development, and cancer metastasis. Zymogen activation is critical for hepsin function. To date, how hepsin is activated and regulated in cells remains an enigma. In this study, we conducted site-directed mutagenesis, cell expression, plasma membrane protein labeling, trypsin digestion, Western blotting, and flow cytometry experiments in human hepatoma HepG2 cells, where hepsin was originally discovered, and SMMC-7721 cells. Our results show that hepsin is activated by autocatalysis on the cell surface but not intracellularly. Moreover, we show that hepsin undergoes ectodomain shedding. In the conditioned medium from HepG2 and SMMC-7721 cells, we detected a soluble fragment comprising nearly the entire extracellular region of hepsin. By testing protease inhibitors, gene knockdown, and site-directed mutagenesis, we identified calpain-1 as a primary protease that acted extracellularly to cleave Tyr52 in the juxtamembrane space of hepsin. These results provide new insights into the biochemical and cellular mechanisms that regulate hepsin expression and activity.
The mass–size plane of EAGLE galaxies
Authors: Rosito M. S. , Tissera P. B. , Pedrosa S. E. , Lagos C. D. P. 
Published: August 2019

Context. Current observational results show that both late- and early-type galaxies follow tight mass–size planes on which physical properties such as age, velocity dispersion, and metallicity correlate with the scatter on the plane.
Aims. We study the mass–size plane of galaxies in cosmological hydrodynamical simulations, as a function of velocity dispersion, age, chemical abundances, ellipticity, and spin parameters with the aim of assessing to what extent the current cosmological paradigm can reproduce these observations and provide a physical interpretation of them.
Methods. We selected a sample of well-resolved galaxies from the (100 Mpc)3 simulation of the EAGLE Project. This sample is composed of 508 spheroid-dominated galaxies and 1213 disc-dominated galaxies. The distributions of velocity dispersion, age, metallicity indicators and gradients, and spin parameters across the mass–size plane are analysed. Furthermore, we study the relation between shape and kinematic parameters. The results are compared with observations.
Results. The mass-weighted ages of the EAGLE galaxies are found to vary along lines of constant velocity dispersion on the mass–size plane, except for galaxies with velocity dispersions higher than ∼150 km s−1. Negative age gradients tend to be found in extended disc galaxies in agreement with observations. However, the age distributions of early-type galaxies show a larger fraction with inverted radial profiles. The distribution of metallicity gradients does not show any clear dependence on this plane. Galaxies with similar spin parameters (λ) display larger sizes as their dynamical masses increase. Stellar-weighted ages are found to be good proxies for λ in galaxies with low ellipticity (ε). A bimodal distribution of λ is found so that the high-λ peak is dominated by discs with young stellar populations (SPs), while the second peak is mainly populated by slow rotators (λ <  0.2) with old stars. Our findings suggest that the physical processes that regulate the star formation histories in galaxies might also affect the angular moment budgets of gas and stars, and as a consequence their morphology.
The LUMBA UVES stellar parameter pipeline
Authors: Gavel Alvin , Gruyters Pieter , Heiter Ulrike , Korn Andreas J. , Lind Karin , Nordlander Thomas 
Published: August 2019

Context. The Gaia-ESO Survey has taken high-quality spectra of a subset of 100 000 stars observed with the Gaia spacecraft. The goal for this subset is to derive chemical abundances for these stars that will complement the astrometric data collected by Gaia. Deriving the chemical abundances requires that the stellar parameters be determined.
Aims. We present a pipeline for deriving stellar parameters from spectra observed with the FLAMES-UVES spectrograph in its standard fibre-fed mode centred on 580 nm, as used in the Gaia-ESO Survey. We quantify the performance of the pipeline in terms of systematic offsets and scatter. In doing so, we present a general method for benchmarking stellar parameter determination pipelines.
Methods. Assuming a general model of the errors in stellar parameter pipelines, together with a sample of spectra of stars whose stellar parameters are known from fundamental measurements and relations, we use a Markov chain Monte Carlo method to quantitatively test the pipeline.
Results. We find that the pipeline provides parameter estimates with systematic errors on effective temperature below 100 K, on surface gravity below 0.1 dex, and on metallicity below 0.05 dex for the main spectral types of star observed in the Gaia-ESO Survey and tested here. The performance on red giants is somewhat lower.
Conclusions. The pipeline performs well enough to fulfil its intended purpose within the Gaia-ESO Survey. It is also general enough that it can be put to use on spectra from other surveys or other spectrographs similar to FLAMES-UVES.
The FED-Method (Fixation, Elongation, Derotation) – a Machine-supported Treatment Approach to Patients with Idiopathic Scoliosis – Systematic Review
Authors: Nisser Jenny , Smolenski Ulrich , Sliwinski Grzegorz Eugeniusz , Schumann Paula , Heinke Andreas , Malberg Hagen , Werner Michael , Elsner Sophia , Drossel Welf-Guntram , Sliwinski Zbigniew , Derlien Steffen 
Published: August 2019

Abstract
Background The FED method (Fixation, Elongation, Derotation) is a treatment method approach to Patients with scoliosis. The FED method is especially established in Spain and Poland, whereby in Germany it is less well-known. Nevertheless the FED method is within the scope of a research project (Project Number: 19200 BR/3). The purpose of the paper is to characterize the FED method and to highlight the specificities in contrast to the Schroth method, which is international established and especially in Germany.
Methods This systematic literature research was conducted in Nov 2017–Jan 2018. Therefore common medical and physiotherapeutic databases were used. Furthermore there was a hand search in selected scientific journals. Only a small number of relevant references were identified. That is why the respective authors were asked to provide the full-texts of their papers and to recommend further references.
Results A total of 378 references were identified. After removing duplicates and the content-related selection, 19 references were deemed to be relevant. Based on the analysis of this relevant literature, the FED method was comprehensively characterized. First of all the general structure of the FED method and the scientific evidence for its effectiveness was described. And as a result of the literature research, the operating principles of the FED method were pointed out. Then these operating principles were discussed in comparison with the Schroth method. The Schroth method based on sensomotoric and kinesthetic principles and the correction of the pathologic posture was performed by selective muscle activation and breathing-pattern. Thus, the posture correction will be performed by the patients (auto correction). Compared to the Schroth method, the FED method implements the posture correction by the FED-device. This correction is influenced by mechanical forces with a comparatively high strength and intensity. The repetitive mechanical correction stimulates the sensomotoric system. And due to trophic/biochemical adaptations, the physiological bone growth will be stimulated.
Conclusion In total the authors want to clarify, that both treatment methods (Schroth method, FED method) supposed to be applied in consideration of the preconditions of the patients and the pursue of the different treatment goals. Thus, the implementation of treatment methods should be used according to the individual treatment demand and on different stages in the treatment process.
Radiographic Osteoarthritis Prevalence Over Ten Years After Anterior Cruciate Ligament Reconstruction
Authors: Chen Tianwu , Wang Shuang , Li Yunxia , Ai Chengchong , Jiang Fangyi , Chen Shiyi 
Published: August 2019

AbstractThe purpose of this study was to conduct an up-to-date systematic review and meta-analysis of radiographic knee osteoarthritis (OA) over minimal ten years after ACL reconstruction. The database of Pubmed and the Ovid was adopted. The radiographic knee OA over minimal ten years after ACL reconstruction was systematically reviewed. Both the ipsilateral and contralateral knees were evaluated referring to the tibiofemoral joint (TFJ), the patellofemoral joint (PFJ), and the overall knee OA prevalence. Nineteen studies were included for review, with nine screened for the meta-analysis. The overall knee OA rate ranged from 8.3–79.2%, meanly 51.6% on the ipsilateral side; ranged from 3.6–35.7%, meanly 15.5% on the contralateral side. Compared to the contralateral side, the RR of developing radiographic OA was 3.73 (P<0.01) for the overall knee, 2.88 (P<0.01) for TFJ, and 2.42 (P<0.01) for PFJ. Ipsilaterally, the RR of developing TFJ radiographic OA was 1.15 (P<0.01) compared to that of the PFJ. Over a minimum of 10 years after surgery, more than half the cases developed overall radiographic OA on the ipsilateral knee, which was nearly four times higher than the contralateral side. On the ipsilateral knee, the TFJ was most affected.
Analyse der Behandlungszahlen in den Münchener Notaufnahmen
des Jahres 2013/2014
Authors: Trentzsch Heiko , Dodt Christoph , Gehring Christian , Veser Alexander , Jauch Karl-Walter , Prückner Stephan ,   Studiengruppe „Runder Tisch Notfallversorgung Landeshauptstadt
                        München“
Published: August 2019

ZusammenfassungDie Notfallversorgung in den deutschen Krankenhaus-Notaufnahmen ist kaum
erforscht. Anlässlich der Restrukturierung des Städtischen
Klinikums München initiierte der Stadtrat den „Runden Tisch
Notfallversorgung“ um Richtzahlen für die
Kapazitätsplanung zu erhalten. Die vorliegende Studie sollte dazu
Behandlungsdaten von 14, an der Notfallversorgung hauptsächlich
beteiligten, Krankenhausnotaufnahmen analysieren. Für stationäre
Fälle wurden Daten nach §21 Krankenhausentgeltgesetz,
für ambulante Fälle – soweit verfügbar –
ähnliche Daten herangezogen, anonymisiert und mit Rettungsdienstdaten
zusammengeführt. Um die Bereiche Behandlungsdringlichkeit,
diagnostischer/therapeutischer Aufwand und Bettenbedarf beschreiben zu
können wurden die Daten jeweils in einem vierstufigen System
kategorisiert. Aus 12 Monaten wurden 524 716 Behandlungsfälle
erfasst: 34% wurden stationär aufgenommen, 80% kamen
ohne zu Hilfenahme des Rettungsdienstes. Jeder siebte Patient, der
selbstständig eine Notaufnahme aufsuchte, benötigte ein
Intensiv- oder Überwachungsbett (ICU/IMC). Pro Tag und 100 000
Einwohner stellten sich 64 Fälle mit einem Bettenbedarf von 7
ICU/IMC und 15 Normalstationsbetten vor. Die meisten Fälle
(66%) wurden ambulant behandelt und kamen zu Zeiten, in denen
kassenärztliche Notfallversorgungseinrichtungen erreichbar
wären. Die Dringlichkeit dieser Fälle war meist gering
(50,9%), der Aufwand oft aber hoch, begründet durch Diagnostik
und chirurgische Versorgungen. Die Studie liefert Grundlagen zur Planung der
Notfallversorgung. Ein Großteil der Vorstellungen scheint insbesondere
bei Verletzungen und bei Krankheitsbildern, die einer differenzierten
Abklärung bedürfen, medizinisch gerechtfertigt weshalb
entsprechende Kapazitäten eingeplant werden müssen. Die Studie
zeigt auch, dass eine Kapazitätsplanung allein auf der Basis der
rettungsdienstlich vorgestellten Fälle eine ungeeignete, weil einseitige
Betrachtungsweise ist.
Toilettenhygiene in Schulen: ein altes und immer noch aktuelles
Thema. Ergebnisse einer Umfrage bei Schulleitungen und Schülern in
Frankfurt am Main, 2018
Authors: Heudorf Ursel , Neuhäusel Tim , Demircan Yunus , Leonhardt Kerstin , Petrozziello Sofiya , Savas Gamze , Steul Katrin 
Published: August 2019

Zusammenfassung
Hintergrund Aufgrund zahlreicher Rückmeldungen zu dem Zustand der
Toiletten an Frankfurter Schulen wurde im Juni 2018 eine Umfrage bei
Schulleitungen und Schülern zu deren Erfahrungen und Sichtweisen
durchgeführt. Ziele der Umfrage waren sowohl eine Bestandserhebung von
Problemlagen als auch die Erfassung von good practice-Beispielen.
Methoden Es wurden ein Fragebogen für die Schulleitungen und ein
weiterer Fragebogen für die Schüler entwickelt, welcher pro
Klasse ausgefüllt werden sollte. Erfahrungen und Vorschläge
wurden sowohl von den Schulleitungen als auch von den Schülern
erfragt
Ergebnisse 147 allgemeinbildende Schulen wurden angeschrieben. 86
Schulleitungen (Teilnahmequote 57,8%) schickten die Fragebögen
für die Leitung zurück. Aus 107 (Teilnahmequote 72,8%)
der Schulen wurden 982 Schüler/Klassen-Fragebogen
ausgefüllt zurückgeschickt. 18,6% der Schulleitungen
waren mit dem Zustand der Toiletten zufrieden; als Erfolgsfaktoren nannten sie
pädagogische Maßnahmen zum Nutzungsverhalten und Bereitstellung
ausreichender Hygieneartikel (je 14%) sowie eine erhöhte
Reinigungsfrequenz (12%). Als Ursache für Probleme gaben
73% der Leitungen das Schülerverhalten an, in weniger als
40% mangelhafte Reinigung. Verbesserungsvorschläge umfassten:
höhere Reinigungsintervalle , zusätzliches Aufsichtspersonal,
Bau/-Renovierungsarbeiten sowie ein höheres Budget für
Hygieneartikel. 10% der 982 Klassen gaben an, dass nichts an ihren
Toiletten störe, 92% der Klassen klagten über Gestank,
85% über Schmutz und 70% über fehlendes
Toilettenpapier. Als Gründe für Probleme gaben die Klassen in
89% das Verhalten der Schüler selbst an.
Verbesserungsvorschläge der Schüler reichen von verbesserter
Reinigung und mehr Reinigungspersonal über Baumaßnahmen, mehr
Kontrolle und Aufsicht usw. bis hin zu technischen Lösungen wie ein
Chip- und Code-System.
Schlussfolgerungen Die Umfrage bestätigt die deutlich
verbesserungsbedürftige Situation rund um die Problematik der
Schultoiletten. Sowohl die Schulleitungen als auch die Klassen nannten am
häufigsten das Nutzerverhalten der Schüler und Vandalismus als
Ursache, mit deutlichem Abstand gefolgt von Problemen mit der Reinigung oder
alten Toiletten. D.h. eine verbesserte Reinigung ist zwar häufig
erforderlich, reicht aber alleine – ohne eine Änderung des
Verhaltens der Schüler – nicht aus. Die vorgeschlagenen
Verbesserungsmaßnahmen sollten getestet und bei Bewährung
implementiert werden.
Präperitoneale umbilikale Netzplastik (PUMP): Indikationen, Technik und Resultate
Authors: Köhler Gernot , Lechner Michael , Kaltenböck Richard , Pfandner Richard , Hartig Nikolaus 
Published: August 2019

Zusammenfassung
Einleitung Das Prinzip der präperitonealen umbilikalen Mesh-Plastik (PUMP) besteht in der extraperitonealen Netzplatzierung hinter der Rektusmuskulatur mit nachfolgendem anterioren Fasziendefektverschluss. Schwierigkeiten können im Rahmen der Dissektion ebenso auftreten wie bei der Netzeinbringung, Entfaltung und Positionierung.
Methodik 81 konsekutive Patienten mit primären umbilikalen oder epigastrischen Hernien zwischen 2 und 4 cm Defektgröße, die zwischen Januar 2015 und März 2018 mittels PUMP-Technik operiert wurden, konnten prospektiv in der Herniamed-Datenbasis erfasst und retrospektiv ausgewertet werden. Es wurde konsistent dieselbe Technik angewendet, aber 3 verschiedene Implantate kamen im Verlauf des Beobachtungszeitraumes zum Einsatz. Diese Arbeit schildert unsere Erfahrungen und Resultate in Bezug auf die jeweils verwendeten Materialien.
Resultate Es gab keine intraoperativen, aber 6 postoperative Komplikationen (6/81 = 7,4%). Drei Patienten mussten ungeplant reoperiert werden. 76 von 81 Patienten (93,8%) waren für die 1-Jahres-Nachkontrolle verfügbar. Drei von 76 Patienten (3,9%) erlitten ein Rezidiv und 5 Patienten (6,6%) berichteten über behandlungsbedürftigen chronischen Schmerz.
Schlussfolgerung Jeder Chirurg sollte mit dem Implantat arbeiten, das am besten seinen Bedürfnissen sowie den klinischen Anforderungen und patientenindividuellen Faktoren gerecht wird. Die PUMP-Technik ist gut geeignet für Ventralhernien zwischen 2 und 4 cm Defektgröße ohne reparationsbedürftige pathologische Rektusdiastase und ermöglicht eine lokale extraperitoneale netzbasierte Defektverstärkung ohne das Risiko von intraperitonealen Komplikationen. Die Rezidivraten können im Vergleich zum Nahtverschluss gesenkt werden, ohne die Komplikationsraten zu erhöhen.