bluuebunny/crossref_metadata_2025_split huggingface dataset:
Omega-3 fatty acids for the treatment of depressive disorders in children and adolescents: a meta-analysis of randomized placebo-controlled trials
Authors: Zhang Li , Liu Huan , Kuang Li , Meng Huaqing , Zhou Xinyu 
Published: September 2019

AbstractBackgroundTo investigate the efficacy and safety of omega-3 fatty acids (O3FA) in treating depressive disorders in children and adolescents.MethodWe conducted a comprehensive search in electronic databases and hand-searched articles included for relevant studies. We included randomized controlled trials which studied on O3FA for treatment of children and adolescents with depression. The standard mean differences (SMDs) and the odds ratios (ORs) with 95% confidence intervals (CIs) were estimated by a random-effects model. The primary outcomes were end-point depressive symptoms scores (efficacy) and all-cause discontinuation (safety). The secondary outcome of response rate was also assessed. Subgroup analyses were performed by age, severity of depression and dosage. Risk of bias assessment was performed based on the Jadad score and the Cochrane Collaboration’s risk-of-bias method.ResultsA total of four studies with 153 participants were included. In terms of efficacy, there was no significant difference of end-point depressive symptoms scores between O3FA and placebo (SMD = − 0.12, 95% CI − 0.53 to 0.30, P = 0.58;I2= 30%). In terms of safety, the all-cause discontinuation showed no statistical significance between O3FA and placebo (OR = 1.3, 95% CI 0.58 to 2.93, P = 0.53;I2= 0%). The response rate of O3FA was also not significant better than that of placebo (OR = 1.57, 95% CI 0.26 to 9.39, P = 0.62;I2= 71%). Besides, there were also no significant differences in those subgroup analyses outcomes. The risk of bias of included trials were not high.ConclusionsOnly considering the limited evidence of O3FA in the acute treatment of major depressive disorder, it did not seem to offer a clear advantage for children and adolescents.
TCR-like antibodies in cancer immunotherapy
Authors: He Qinghua , Liu Zhaoyu , Liu Zhihua , Lai Yuxiong , Zhou Xinke , Weng Jinsheng 
Published: September 2019

AbstractCancer immunotherapy has been regarded as the most significant scientific breakthrough of 2013, and antibody therapy is at the core of this breakthrough. Despite significant success achieved in recent years, it is still difficult to target intracellular antigens of tumor cells with traditional antibodies, and novel therapeutic strategies are needed. T cell receptor (TCR)-like antibodies comprise a novel family of antibodies that can recognize peptide/MHC complexes on tumor cell surfaces. TCR-like antibodies can execute specific and significant anti-tumor immunity through several distinct molecular mechanisms, and the success of this type of antibody therapy in melanoma, leukemia, and breast, colon, and prostate tumor models has excited researchers in the immunotherapy field. Here, we summarize the generation strategy, function, and molecular mechanisms of TCR-like antibodies described in publications, focusing on the most significant discoveries.
Cryptosporidium infections in terrestrial ungulates with focus on livestock: a systematic review and meta-analysis
Authors: Hatam-Nahavandi Kareem , Ahmadpour Ehsan , Carmena David , Spotin Adel , Bangoura Berit , Xiao Lihua 
Published: September 2019

Abstract

Background
Cryptosporidium spp. are causative agents of gastrointestinal diseases in a wide variety of vertebrate hosts. Mortality resulting from the disease is low in livestock, although severe cryptosporidiosis has been associated with fatality in young animals.

Methods
The goal of this systematic review and meta-analysis was to review the prevalence and molecular data on Cryptosporidium infections in selected terrestrial domestic and wild ungulates of the families Bovidae (bison, buffalo, cattle, goat, impala, mouflon sheep, sheep, yak), Cervidae (red deer, roe deer, white-tailed deer), Camelidae (alpaca, camel), Suidae (boar, pig), Giraffidae (giraffes) and Equidae (horses). Data collection was carried out using PubMed, Scopus, Science Direct and Cochran databases, with 429 papers being included in this systematic analysis.

Results
The results show that overall 18.9% of ungulates from the investigated species were infected with Cryptosporidium spp. Considering livestock species (cattle, sheep, goats, pigs, horses and buffaloes), analysis revealed higher Cryptosporidium infection prevalence in ungulates of the Cetartiodactyla than in those of the Perissodactyla, with cattle (29%) being the most commonly infected farm animal.

Conclusions
Overall, the investigated domestic ungulates are considered potential sources of Cryptosporidium contamination in the environment. Control measures should be developed to reduce the occurrence of Cryptosporidium infection in these animals. Furthermore, literature on wild populations of the named ungulate species revealed a widespread presence and potential reservoir function of wildlife.
Comparative study of excretory–secretory proteins released by Schistosoma mansoni-resistant, susceptible and naïve Biomphalaria glabrata
Authors: Fogarty Conor E. , Zhao Min , McManus Donald P. , Duke Mary G. , Cummins Scott F. , Wang Tianfang 
Published: September 2019

AbstractBackgroundSchistosomiasis is a harmful neglected tropical disease caused by infection withSchistosomaspp., such asSchistosoma mansoni.Schistosomamust transition within a molluscan host to survive. Chemical analyses of schistosome-molluscan interactions indicate that host identification involves chemosensation, including naïve host preference. Proteomic technique advances enable sophisticated comparative analyses between infected and naïve snail host proteins. This study aimed to compare resistant, susceptible and naïveBiomphalaria glabratasnail-conditioned water (SCW) to identify potential attractants and deterrents.MethodsBehavioural bioassays were performed onS. mansonimiracidia to compare the effects of susceptible, F1 resistant and naïveB. glabrataSCW. The F1 resistant and susceptibleB. glabrataSCW excretory–secretory proteins (ESPs) were fractionated using SDS-PAGE, identified with LC-MS/MS and compared to naïve snail ESPs. Protein-protein interaction (PPI) analyses based on published studies (including experiments, co-expression, text-mining and gene fusion) identifiedS. mansoniandB. glabrataprotein interaction. Data are availableviaProteomeXchange with identifier PXD015129.ResultsA total of 291, 410 and 597 ESPs were detected in the susceptible, F1 resistant and naïve SCW, respectively. Less overlap in ESPs was identified between susceptible and naïve snails than F1 resistant and naïve snails. F1 resistantB. glabrataESPs were predominately associated with anti-pathogen activity and detoxification, such as leukocyte elastase and peroxiredoxin. SusceptibleB. glabrataseveral proteins correlated with immunity and anti-inflammation, such as glutathione S-transferase and zinc metalloproteinase, andS. mansonisporocyst presence. PPI analyses found that uncharacterisedS. mansoniprotein Smp_142140.1 potentially interacts with numerousB. glabrataproteins.ConclusionsThis study identified ESPs released by F1 resistant, susceptible and naïveB. glabratato explainS. mansonimiracidia interplay. SusceptibleB. glabrataESPs shed light on potentialS. mansonimiracidia deterrents. Further targeted research on specific ESPs identified in this study could help inhibitB. glabrataandS. mansoniinteractions and stop human schistosomiasis.
Pneumocystis pneumonia in patients with rheumatic diseases receiving prolonged, non-high-dose steroids—clinical implication of primary prophylaxis using trimethoprim–sulfamethoxazole
Authors: Park Jun Won , Curtis Jeffrey R. , Kim Min Jung , Lee Hajeong , Song Yeong Wook , Lee Eun Bong 
Published: September 2019

Abstract

Objectives
To investigate the incidence of pneumocystis pneumonia (PCP) and its risk factors in patients with rheumatic disease receiving non-high-dose steroid treatment, along with the risks and benefits of PCP prophylaxis.

Methods
This study included 28,292 treatment episodes with prolonged (≥ 4 weeks), non-high-dose steroids (low dose [< 15 mg/day, n = 27,227] and medium dose [≥ 15 to < 30 mg/day, n = 1065], based on prednisone) over a 14-year period. Risk factors for PCP and prophylactic effect of trimethoprim–sulfamethoxazole (TMP-SMX) were investigated if the 1-year incidence rate (IR) of PCP in each dose group was > 0.1/100 person-years. Cox regression with LASSO was used for analysis.

Results
One-year PCP IR in the low-dose group was 0.01 (95% CI 0.001–0.03)/100 person-years, and only the medium-dose group showed eligible PCP IR for further analysis. In the medium-dose group, prophylactic TMP-SMX was administered in 45 treatment episodes while other episodes involved no prophylaxis (prophylaxis group vs. control group). In 1018.0 person-years, 5 PCP cases occurred exclusively in the control group, yielding an IR of 0.5 (0.2–1.2)/100 person-years. Concomitant steroid-pulse treatment and baseline lymphopenia were the most significant risk factors for PCP. Treatment episodes with at least one of these factors (n = 173, high-risk subgroup) showed higher 1-year PCP IR (3.4 (1.1–8.0)/100 person-years), while no PCP occurred in other treatment episodes. TMP-SMX numerically reduced the risk (adjusted HR = 0.2 (0.001–2.3)) in the high-risk subgroup. The IR of adverse drug reactions (ADRs) related to TMP-SMX was 41.5 (22.3–71.6)/100 person-years, including one serious ADR. The number needed to treat with TMP-SMX to prevent one PCP in the high-risk subgroup (31 (17–226)) was lower than the number needed to harm by serious ADR (45 (15–∞)).

Conclusion
Incidence of PCP in patients with rheumatic diseases receiving prolonged, medium-dose steroids depends on the presence of risk factors. Prophylactic TMP-SMX may have greater benefit than potential risk in the high-risk subgroup.
Sustained remission with methotrexate monotherapy after 22-week induction treatment with TNF-alpha inhibitor and methotrexate in early psoriatic arthritis: an open-label extension of a randomized placebo-controlled trial
Authors: de Jong Henriëtte M. Y. , van Mens Leonieke J. J. , Nurmohamed Michael T. , Kok Marc R. , van Kuijk Arno W. R. , Baeten Dominique L. P. , van de Sande Marleen G. H. 
Published: September 2019

Abstract

Background
If TNF inhibitors are initiated in the early stages of psoriatic arthritis, this could potentially modulate disease and therefore allow us to discontinue the TNF inhibitor after achieving remission.

Objective
To investigate whether remission induced by tumour necrosis factor alpha inhibitor (TNFi) and methotrexate in patients with early psoriatic arthritis is sustained after withdrawal of TNFi.

Methods
Open-label extension of a recently published double-blind, randomized placebo-controlled trial. Patients with psoriatic arthritis fulfilling the CASPAR criteria and with active disease at baseline (swollen and tender joint count ≥ 3) were randomized to either golimumab and methotrexate or matched placebo and methotrexate. Patients in Disease Activity Score (DAS) remission at week 22 continued in the open-label extension on methotrexate monotherapy. The primary end point was the percentage of patients in DAS-CRP remission (DAS < 1.6) at week 50.

Results
Eight patients from the original placebo group and 18 patients from the original TNFi group continued in the extension phase. At week 50, 6 out of 8 (75%) patients from the original MTX (methotrexate) group versus 10 out of 18 (56%) patients from the original MTX+TNFi group were in DAS-CRP remission (p = 0.347). Considering the total study population, 6 out of 24 (25%) of the original MTX group versus 10 out of 26 (38.5%) of the original MTX+TNFi group were in DAS remission at week 50 (p = 0.308).

Conclusions
Remission achieved by initial combination treatment with TNFi and methotrexate in early psoriatic arthritis is maintained on methotrexate monotherapy in approximately half of the patients.

Trial registration
Registered at Clinicaltrials.gov with number NCT01871649 on June 7, 2013.
A complex teamwork intervention in a surgical ward in Norway
Authors: Aaberg Oddveig Reiersdal , Hall-Lord Marie Louise , Husebø Sissel Iren Eikeland , Ballangrud Randi 
Published: September 2019

Abstract
Objectives
Interprofessional team training has a positive impact on team behavior and patient safety culture. The overall objective of the study was to explore the impact of an interprofessional teamwork intervention in a surgical ward on structure, process and outcome. In this paper, the implementation of the teamwork intervention is reported to expand the understanding of the future evaluation results of this study.

Results
The evidence-based Team Strategies and Tools to Enhance Performance and Patient Safety (TeamSTEPPS) program was implemented in three phases according to the program’s implementation plan, which are built on Kotter’s organizational change model. In the first phase, a project group with the leaders and researchers was established and information about the project was given to all health care personnel in the ward. The second phase comprised 6 h interprofessional team training for all frontline health care personnel followed by 12 months implementation of TeamSTEPPS tools and strategies. In the third phase, the implementation of the tools and strategies continued, and refresher training was conducted.
Trial registration Trial registration number (TRN) is ISRCTN13997367. The study was registered retrospectively with registration date May 30, 2017
Determinants of stillbirth in Felege-Hiwot comprehensive specialized referral hospital, North-west, Ethiopia, 2019
Authors: Worede Daniel Tarekegn , Dagnew Gizachew Worku 
Published: September 2019

Abstract

Objective
The objective of this study was to identify determinants of stillbirth in Felege Hiwot comprehensive specialized referral hospital, North-west, Ethiopia: 2019. To conduct this study an institutional-based unmatched case–control study was used among 84 cases and 336 controls. Pretested, structured questioner with face to face interview was conducted and some data were also extracted from medical records using a checklist. The data were analyzed by using binary logistics regression. A p-value of < 0.05 was considered as significant at 95% confidence level and the strength of association was measured using odds ratio.

Results
Illiteracy (AOR 3.8, 95% CI 1.4–10.2), sexually transmitted infection (AOR 5.7, 95% CI 1.1–29.7), Premature rupture of membrane (AOR 4.0, 95% CI 1.4–11.3), congenital anomaly (AOR 10.4, 95% CI 2.0–11.2) and history of perinatal death (AOR 10.4, 95% CI 3.7–29.2) were the determinants of stillbirth that increase risk of fetal death. Whereas taking at least two doses of tetanus toxoid vaccine (AOR 0.5, 95% CI 0.2–0.9) and partograph use (AOR 0.2, 95% CI 0.1–0.4) were found to be protective factors for stillbirth. To overcome this problem; empowering female education, facilitating women in taking tetanus toxoid vaccine, sexually transmitted infection prevention, and encourage health professionals to use partograph during labour follow up highly strongly recommended.
Acute human bocavirus 1 infection in child with life-threatening bilateral bronchiolitis and right-sided pneumonia: a case report
Authors: Ziemele Inga , Xu Man , Vilmane Anda , Rasa-Dzelzkaleja Santa , Hedman Lea , Hedman Klaus , Söderlund-Venermo Maria , Nora-Krukle Zaiga , Murovska Modra , Gardovska Dace 
Published: September 2019

Abstract

Background
Human bocavirus 1 is a commonly detected human parvovirus. Many studies have shown human bocavirus 1 as a pathogen in association with acute respiratory tract infections in children. However, because human bocavirus 1 persists in the upper airways for extensive time periods after acute infection, the definition and diagnostics of acute human bocavirus 1 infection is challenging. Until now, detection of human bocavirus 1 exclusively, high viral load in respiratory samples, and viremia have been associated with a clinical picture of acute respiratory illness. There are no studies showing detection of human bocavirus 1 messenger ribonucleic acid in the peripheral blood mononuclear cells as a diagnostic marker for acute lower respiratory tract infection.

Case presentation
We report the case of a 17-month-old Latvian boy who presented in intensive care unit with acute bilateral bronchiolitis, with a history of rhinorrhea and cough for 6 days and fever for the last 2 days prior to admission, followed by severe respiratory distress and tracheal intubation. Human bocavirus 1 was the only respiratory virus detected by a qualitative multiplex polymerase chain reaction panel. For the diagnosis of acute human bocavirus 1 infection, both molecular and serological approaches were used. Human bocavirus 1 deoxyribonucleic acid (DNA) was detected simultaneously in nasopharyngeal aspirate, stool, and blood, as well as in the corresponding cell-free blood plasma by qualitative and quantitative polymerase chain reaction, revealing high DNA-copy numbers in nasopharyngeal aspirate and stool. Despite a low-load viremia, human bocavirus 1 messenger ribonucleic acid was found in the peripheral blood mononuclear cells. For detection of human bocavirus 1-specific antibodies, non-competitive immunoglobulin M and competitive immunoglobulin G enzyme immunoassays were used. The plasma was positive for both human bocavirus 1-specific immunoglobulin M and immunoglobulin G antibodies.

Conclusions
The presence of human bocavirus 1 genomic DNA in blood plasma and human bocavirus 1 messenger ribonucleic acid in peripheral blood mononuclear cells together with human bocavirus 1-specific immunoglobulin M are markers of acute human bocavirus 1 infection that may cause life-threatening acute bronchiolitis.
Response mechanism of hypocrellin colorants biosynthesis by Shiraia bambusicola to elicitor PB90
Authors: Du Wen , Sun Chunlong , Wang Baogui , Wang Yanmei , Dong Bin , Liu Junhua , Xia Jiangbao , Xie Wenjun , Wang Jun , Sun Jingkuan , Liu Xuehong , Wang Hongguo 
Published: September 2019

Abstract
The valuable medicine Shiraia bambusicola P. Henn. and its major active substance hypocrellin exert unique curative effects on skin diseases, diabetes, and cancers. The wild S. bambusicola is endangered due to its harsh breeding conditions and long growth cycle. It is one of the effective ways to utilize the resources sustainably to produce hypocrellin by fermentation of S. bambusicola. PB90 is a protein elicitor isolated from Phytophthora boehmeriae to induce the useful metabolites production in fungi. In this work, PB90 was selected to promote the synthesis hypocrellin by S. bambusicola. To evaluate the effect of PB90 on S. bambusicola, it was found that the induced cells showed decreased biomass, increased cell wall permeability, rapid induction of secondary metabolites, and significant increase of some enzyme activities, which confirmed a strong activation of phenylalanine/flavonoid pathways. Studies on signal molecules and gene expression level in S. bambusicola treated with PB90 have found that hydrogen peroxide (H2O2) and nitric oxide (NO) are necessary signal molecules involved in the synthesis of hypocrellin in elicited cells, and increased their signal levels through mutual reaction. We have showed for the first time, the response mechanism of hypocrellin biosynthesis from S. bambusicola to PB90, which may be not only establish a theoretical foundation for the application of PB90 to the mass production of S. bambusicola, but can also motivate further research on the application of PB90 to the conservation and sustainable utilization of other medical fungi.
The differences of bacterial communities in the tissues between healthy and diseased Yesso scallop (Patinopecten yessoensis)
Authors: Yu Zichao , Liu Chao , Fu Qiang , Lu Guangxia , Han Shuo , Wang Lingling , Song Linsheng 
Published: September 2019

AbstractThe tissues of marine invertebrates are colonized by species-rich microbial communities. The dysbiosis of host’s microbiota is tightly associated with the invertebrate diseases. Yesso scallop (Patinopecten yessoensis), one of the most important maricultured scallops in northern China, has recently suffered massive summer mortalities, which causes huge production losses. The knowledge about the interactions between the Yesso scallop and its microbiota is important to develop the strategy for the disease prevention and control. In the present study, the bacterial communities in hemolymph, intestine, mantle and adductor muscle were compared between the healthy and diseased Yesso scallop based on the high-throughput sequencing of 16S rRNA gene. The results indicated obvious difference of the composition rather than the diversity of the bacterial communities between the healthy and diseased Yesso scallop.Vibrio,FrancisellaandPhotobacteriumwere found to overgrow and dominate in the mantle, adductor muscle and intestine of the diseased scallops, respectively. The prediction of bacterial community metagenomes and the variations of KEGG pathways revealed that the proportions of the pathways related with neurodegenerative diseases and carbohydrate metabolism both increased significantly in the mantle and hemolymph of the diseased scallops. The abundance of the metabolism pathways including carbohydrate metabolism, lipid metabolism and amino acid metabolism decreased significantly in the intestine of diseased scallops. The results suggested that the changes of bacterial communities might be closely associated with the Yesso scallop’s disease, which was helpful for further investigation of the pathogenesis as well as prevention and control of the disease in Yesso scallop.
Effect of different combinations of antibiotics on fruit quality and antioxidant defense system in Huanglongbing infected Kinnow orchards
Authors: Hussain Sajjad , Rao Muhammad Junaid , Anjum Muhammd Akbar , Ejaz Shaghef , Umar Ummad-ud-Din , Ali Muhammad Arif , Khalid Muhammad Fasih , Sohail Muhammad , Ercisli Sezai , Zia-Ul-Haq Muhammad , Ahmad Shakeel , Naqvi Syed Atif Hasan 
Published: September 2019

Abstract
Huanglongbing (HLB), also known as citrus greening disease, is the most devastating disease of citrus across the world, caused by the phloem limited fastidious bacterium ‘Candidatus Liberibacter spp.’. This research was conducted on HLB infected 10-year-old Kinnow orchard located at Multan, Pakistan. Different classes of antibiotics in various combinations were applied on HLB-infected trees. The antibiotic treatments were applied before flowering in February, during fruit setting in April and at fruit growth stage in June. The different antibiotics combinations used were Ampicillin sodium + Rifampicin, Cefalexin + Rifampicin, Ampicillin sodium + Cefalexin, Ampicillin sodium + Cefalexin + Rifampicin and Control (distilled water). Different fruit qualitative and quantitative attributes were examined. The application of antibiotics significantly decreased 2–11% in flower, June and pre-harvest drops as compared to control. Further, antibiotics increased fruit weight and yield by five times while the juice content, total soluble solids, ripening index, total sugars, phenolic and vitamin C content were also increased in fruits. In addition, total soluble proteins, peroxidase and catalase activities were increased in fruits harvested from antibiotic treated plants compared to control, however the superoxidase dismutase activity was decreased in fruits of antibiotic treated plants. Finally, it is concluded that application of different antibiotics combinations helps in improving the fruit yield and different quality attributes of HLB infected Kinnow trees.