bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum adiponectin level is negatively related to insulin resistance in women with polycystic ovary syndrome
Authors: Yang Jie , Lin Min , Tian Xiaoyan , Li Chujun , Wu Haocun , Deng Ling , Li Xuelan , Chen Xin 
Published: October 2024

PurposeOur study aimed to assess the relationship between serum adipokines and insulin resistance (IR) in women with polycystic ovary syndrome (PCOS) and explore the predictive value of adipokines on IR in PCOS.MethodsThis was a prospective cross-sectional study. 154 women with PCOS were included from July 2021 to September 2022 who underwent gonadal steroid hormone measurement, lipid profile, oral glucose tolerance test and homoeostasis model assessment (HOMA)-IR. Adiponectin (APN), leptin and secreted frizzled-related protein (Sfrp5) were measured by immunoturbidimetry and enzyme-linked immunosorbent assay. Women with PCOS were categorised based on the presence of IR.ResultsWomen with PCOS with IR (n = 99) had significantly lower APN level and APN-to-leptin ratio (A/L ratio) than those without IR (n = 55), whereas serum levels of leptin and Sfrp5 were similar between the two groups. In multivariable linear regression analysis, serum log (APN) and log (A/L ratio) were associated with log (HOMA-IR), and the association was statistically significant after adjusting for body mass index and free androgen index. The area under the receiver operating characteristic curve (95% CI) for APN and A/L ratio was 0.726 (0.644–0.807; P < 0.001) and 0.660 (0.569–0.751; P < 0.01), respectively, with cutoff values of 5.225 mg/L (Youden index ¼ 0.364) and 1.438 mg/L (Youden index ¼ 0.265).ConclusionOur study demonstrated that serum APN was negatively related to IR. Serum APN may be useful as a clinical marker for IR in women with PCOS. Our findings warrant further investigations into the function of APN in the pathogenesis of IR in women with PCOS.
Exploration of the shared gene signatures and comorbidity mechanisms of primary aldosteronism and atrial fibrillation
Authors: Wan Jindong , Liu Sen , Luo Tao , Yang Yi , Wang Dan , Wang Xinquan , Zhou Peng , Hou Jixin , Wang Peijian 
Published: October 2024

BackgroundPrimary aldosteronism (PA) is a prevalent cause of endocrine hypertension characterized by an excess of aldosterone that can induce proinflammatory, prooxidant and profibrotic effects on the heart. Emerging evidence indicates a heightened incidence of atrial fibrillation (AF) in patients with PA, suggesting a significant association between the two conditions. However, the underlying mechanisms remain unclear. The purpose of this study was to investigate the molecular networks associated with the development of both PA and AF.MethodsDatasets were obtained from the Gene Expression Omnibus database. Hub genes were identified by enrichment and protein–protein interaction analysis. These hub genes were subsequently validated via two independent external datasets: GSE60042 (PA microarray dataset) and GSE41177 (AF microarray dataset). Following the identification of shared genes, quantitative real-time polymerase chain reaction (qPCR) was employed to verify the reliability of the dataset and to further confirm the presence of shared genes in clinical samples.ResultsThe results of the common gene analysis revealed that immune and inflammatory responses may be shared features in the pathophysiology of PA and AF. One hub gene, specifically tumor necrosis factor superfamily member 10 (TNFSF10), was identified through various analyses and subsequently validated via qPCR. Compared with that in healthy controls, the expression level of TNFSF10 was lower in PA patients with AF.ConclusionOur findings indicate that TNFSF10 may play a role in the pathophysiology of AF complications associated with PA conditions, suggesting that it could serve as a potential target for the diagnosis or treatment of PA patients complicated with AF.
Brain-derived neurotrophic factor stimulates hypothalamic and gonadal reproductive hormones and oocyte maturation in zebrafish
Authors: Uju Chinelo , Karimzadeh Katayoon , Unniappan Suraj 
Published: October 2024

In briefThe brain-derived neurotrophic factor (BDNF) has been shown to regulate metabolism in zebrafish. This research shows that Bdnf is a regulator of female zebrafish reproduction and reproductive hormones.AbstractBDNF is a peptide widely known for its role in neurogenesis and synaptic plasticity. Its expression in non-neuronal tissues has been reported. In mammals, it is involved in ovarian development, follicle growth, oocyte maturation and early embryonic development. In zebrafish, it was demonstrated that Bdnf increases food intake and regulates metabolism. Reproduction and metabolism are tightly linked. We hypothesized that Bdnf modulates reproductive hormones and reproductive functions in zebrafish. This study aimed to determine Bdnf expression in the zebrafish reproductive axis and whether it modulates the reproductive endocrine milieu and oocyte biology in zebrafish. Our results show that bdnf and its receptor trkb and Bdnf-like immunoreactivity are present in zebrafish gonads and liver cells. This suggests Bdnf local production and possible actions within the gonads and liver. Intraperitoneal administration of 1, 10 or 100 ng/g body weight BDNF significantly (ANOVA, P < 0.05) increased sgnrh/cgnrh-II, kiss1 and cyp19a1b mRNAs in the zebrafish brain, steroidogenic enzymes (star and cyp19a1a) and key receptors in the zebrafish gonads. In vitro incubation of zebrafish liver cells with BDNF significantly (ANOVA, P < 0.05) increased estrogen receptor mRNAs and vitellogenin concentrations (ELISA) in the cells. BDNF (100 ng/mL) induced (ANOVA, P < 0.05) oocyte maturation in vitro at 24 h post-incubation and significantly upregulated cumulus-expansion-related genes (ANOVA, P < 0.05). Overall, our findings indicate a stimulatory role for Bdnf in the reproductive axis of zebrafish. This provides impetus for future research on its mechanism of action and potential practical applications to enhance reproduction in aquaculture.
The Effect of Corticosteroids on the Progression and Outcomes of Polytrauma in Children
Authors: Pshenisnov Konstantin V. , Aleksandrovich Yury S. , Lipin Andrey S. 
Published: October 2024

Polytrauma in children are among the most common causes of death in the pediatric intensive care unit (ICU).The aim of this study was to evaluate the effect of systemic corticosteroids (SCS) on the progression, laboratory parameters, and outcomes of severe multiple injuries in children requiring ICU.Materials and methods. A retrospective, observational, multicenter (case-control and cross-sectional) study included 203 patients from pediatric ICUs across the Russian Federation. The Abbreviated Injury Scale (AIS) score was 36.81 (25–48), and the Pediatric Trauma Score (PTS) was 5.2 (2–8). SCS were administered to 113 (55.7%) children, 19 (9.36%) of whom died.Results. The most severe changes in laboratory parameters, such as an increase in amylase (35.3 vs. 18.3; P0.001) and activated partial thromboplastin time (APTT) (28.9 vs. 25.8; P0.001), were documented upon admission of children with multiple traumatic injuries to the hospital compared with subsequent days of treatment in the ICU. The average fluid volume (as a percentage of age-related fluid requirements) on the first day of treatment in the ICU was 118.53% and did not exceed 84.42% on subsequent days (P0.001). Higher systolic blood pressure (SBP) during the first three days of ICU treatment was observed in children treated without SCS. SBP tended to decrease by day 5, and then a tendency toward arterial hypertension emerged on days 6–7. In children treated with SCS, blood pressure remained stable during the first seven days in the ICU, contributing to a favorable outcome.Conclusion. The use of SCS in children with severe polytrauma from the first day of ICU treatment contributed to the stabilization of hemodynamic parameters and improved control of shock signs. A positive response to SCS in these patients can be considered a marker for a favorable disease course during ICU treatment.
Effect of Succinate Crystalloid Solution on Hemostasis in Children with Severe Community-acquired Pneumonia
Authors: Lazarev Vladimir V. , Anchutin Pavel E. , Megeryan Manuel M. , Bykov Mikhail V. , Smirnov Dmitry A. , Pchelicnceva Tatiana A. , Frolov Nikolay S. , Makhachilaeva Khurzada M. , Golubev Boris I. , Spiridonova Elena A. 
Published: October 2024

Aim of the study. To improve outcomes in children with severe community-acquired pneumonia (CAP) by including succinate-containing crystalloid solution (SCCS) in the treatment plan.Materials and methods. The study included 100 patients diagnosed with CAP. SCCS was administered to 24 patients from the prospective (main) group, divided into 2 equal subgroups of 12 subjects who received SCCS with the infusion rate of 2.5 ml/kg/h (subgroup 1) and 5.0 ml/kg/h (subgroup 2). Treatment of 76 patients in the retrospective (control) group did not include SCCS.Results. Greater decreases in D-dimer (by 418.5 ng/mL vs. 137.0 ng/mL, P=0.026) by day 3 and in fibrinogen (by 1.7 g/L vs. 0.2 g/L, P0.001) by day 3 and (3.8 g/L vs. 0.5 g/L, P=0.002) by day 5 of hospitalization were found in children from the main group vs. the control group. Fibrinogen levels decreased in both study subgroups, although subgroup 1 had significantly higher fibrinogen levels on day 2 of ICU stay (P=0.034). A significant increase in activated partial thromboplastin time (aPTT) of 9.7 seconds was observed on day 3 in the main group versus 2.9 seconds in the control group (P0.001). There was a direct correlation between fibrinogen level and neutrophil count on day 2 of ICU stay (R=0.479, P=0.033). Conclusion. The use of SCCS in the treatment of severe CAP helps to prevent thrombotic complications, reduces hypoxia-induced changes in the coagulation system, and enhances the effects of unfractionated heparin. SCCS infusion at a rate of 5.0 mL/kg/h effectively reduces the levels of hypercoagulation markers, while its administration at a rate of 2.5 ml/kg/h potentiates the effects of unfractionated heparin. The effects of SCCS on hemostasis in severe CAP are equivalent to those of a moderate anticoagulant.
Ultrasound-Based Cardiac Output Monitoring During Pediatric Open-Heart Surgery in Children
Authors: Soloviev Nikolay A. , Rybka Mikhail M. , Khinchagov Jumber Ya. , Tsoi Sofya M. , Gorbunov Gleb E. , Dibin Denis A. , Kodzokov Zera A. , Chomaeva Madina Yu. 
Published: October 2024

Aim of the study. To evaluate the feasibility of using non-invasive hemodynamic monitoring technology based on Doppler ultrasound during open-heart surgery in children.Material and methods. Prospective, observational, single-center cohort study included 20 patients aged 10 to 34 months undergoing surgery for congenital heart defects. Ten patients underwent atrial septal defect closure (ASD group), other 10 patients had ventricular septal defect closure (VSD group). Cardiac output (CO) was measured in all patients to guide inotropic and infusion therapy adjustments at three control time points: (1) after intubation and before skin incision, (2) during the immediate post-bypass period with the chest open after weaning from cardiopulmonary bypass (CPB), and (3) after sternal closure and before transfer to the intensive care unit (ICU).Results. At time point 1, the CO values for both the ASD and VSD groups were within the normal reference range: 5.2 L/min [4.7; 5.5] and 5.1 L/min [4.6; 5.6], respectively. At time point 2, CO was measured in 15 of 20 patients, including 8 patients in the ASD group and 7 in the VSD group. Coverage was 75% because of the challenges of measuring 5 patients on the operating table. In the immediate post-bypass period, two patients with VSD (25%) developed hypotension with CO reduced to 3.6 L/min, which is lower than the age-related hemodynamic reference value (5.1 L/min). Inotropic support in these two patients was increased by switching from dopamine, 7 mcg/kg/min, to adrenaline at a dose of 0.05 mcg/kg/min, resulting in improvement of hemodynamic parameters and an increase in CO to 5.2 L/min and 5.0 L/min, respectively, compared to normal agerelated reference values (4.1; 6.1 L/min). After sternal closure, CO values in both groups did not differ significantly from age-related reference values.Conclusion. The USCOM cardiac output monitoring device can be used to manage intraoperative hemodynamics and adjust inotropic therapy even during open chest surgery. However, its routine use in all stages of surgery with median sternotomy is difficult because it requires more time to align the aortic valve projection.
Efficacy and Safety of a Standardized CPAP Protocol in the Delivery Room in Late Preterm Infants with Infectious and Non-Infectious Lung Diseases
Authors: Shestak Eugene V. , Kovtun Olga P. , Mylarshikova Ekaterina A. , Nechaeva Yulia I. 
Published: October 2024

The aim of this study was to evaluate the efficacy and safety of a standardized protocol of delivery room CPAP therapy in late preterm infants with acute neonatal respiratory failure (ARF) caused by various conditions.Material and methods. A retrospective comparative study of the efficacy of the standardized CPAP protocol in the cohorts of late preterm infants (34–36 weeks) was conducted at the Yekaterinburg Perinatal Center. The comparison group (C, N=256) included infants who received CPAP therapy in the delivery room during 12 months in 2020 before the introduction of the standardized protocol. The study group (S, N=169) included infants treated with standardized CPAP in April-December, 2022. The following subgroups were identified in groups C and S based on the cause of ARF: transient tachypnea of the newborn (TTN; C: N=100; S: N=89), respiratory distress syndrome (RDS; C: N=84; S: N=39), and congenital infection (CI; C: N=54; S: N=37). Other causes of ARF in groups C and S were found in 18 and 4 infants, respectively.Results. Switching to the standardized CPAP protocol reduced the duration of mechanical ventilation by an average of 24 h (P=0.013), the incidence of documented cerebral ischemia (CI) from 64.1% to 53.2% in all subgroups (P=0.022), the length of stay in the neonatal ward from 12 to 11 days (P=0.001), and the length of stay in the hospital from 16 to 14 days (P=0.001) as well as the incidence of CI in the STTN subgroup vs CTTN (38.2% vs. 61.0%, P=0.002). No significant differences were found in the RDS and CI subgroups. The frequency and duration of binasal CPAP and lung ventilation in the neonatal ICU did not differ between subgroups. Pneumothorax within the first 24 h occurred in one patient in group C and in two patients in group S (P=0.339), all of whom were diagnosed with congenital infection. No damage to the nasal passages was observed in any group. Conclusion. The use of a standardized protocol of CPAP therapy for neonates born after 35 weeks of gestation with respiratory failure of any etiology can significantly reduce the severity and duration of illness and should be considered as a basic respiratory strategy in the delivery room when indicated.
Incorporating silica nanoparticles with silver patches into alginate-based bioinks for 3D bioprinting
Authors: Anspach Annalise , Bider Faina , Völkl Andreas R. , Klupp Taylor Robin N. , Boccaccini Aldo R. 
Published: October 2024

Abstract
Alginate dialdehyde-gelatin (ADA-GEL) hydrogels are being studied in bioprinting for their combination of cell adhesion and printability. To incorporate specific functionalities and to improve printability, different additives to ADA-GEL inks are being proposed. Here, a novel type of functional nanoparticles comprising silver patches on silica cores was incorporated into ADA-GEL bioinks. Silver patchy particles (SPPs) were present both on the surface and interior of printed structures. Incorporation of SPPs improved printability of ADA-GEL inks and supported osteosarcoma (MG-63) cells over 7 days of culture. SPPs represent a valuable additive for ADA-GEL hydrogels, being attractive to develop bioinks with advanced functionalities.

Graphical abstract
Determination of voltage quality indicators in industrial power supply systems with limited energy system capacity
Authors: Papaika Yu.A. , Lysenko O.G. , Malyshko M.M. , Udovyk O.V. 
Published: October 2024

Purpose. To justify the dependence of the determination of indicators of electromagnetic compatibility of power supply systems of industrial enterprises in the presence of powerful nonlinear and asymmetric loads in conditions of limited power system capacity.
Methodology. Methods of theoretical electrical engineering for the construction of replacement schemes of three-phase electrical networks, Fourier series for the analysis of higher harmonics of current and voltage, the method of symmetrical components for the analysis of asymmetry.
Findings. Виділено основні показники якості напруги, значення яких залежить від параметрів живлячої електроенергетичної системи (потужності короткого замикання). Наведено аналітичні залежності розрахунку показників несиметрії та несинусоїдальності при обмеженні потужності КЗ. Проаналізовано засоби корекції показників якості в умовах електричних мереж з розподіленою генерацією.
Originality. The scientific novelty consists in establishing the patterns of changes in the mode parameters of the industrial load from the parameters of the power system, which is expressed in interrelated changes in voltage quality indicators. At the same time, in the analytical models, the option of obtaining variable changes in the parameters of the power system both due to the generating capacities and through the parameters of the substitution schemes of electrical networks of different voltage classes has been added.
Practical value. For engineering calculations, simplified algorithms for determining voltage quality indicators (deviation, oscillation, asymmetry and non-sinusoidal voltage) are proposed, taking into account the real configuration of the electrical network and the actual power of the power system. This makes it possible to evaluate the efficiency of transmission and conversion of electricity under an arbitrary configuration of the post-emergency power supply scheme.
Evaluation of Copper and Gold Wire Bonds in PEM Devices
Authors: Myer Brian , Gallardo Eric , Aldridge Dustin , Melosky Stephen 
Published: October 2024

Abstract
The shift from gold to copper wire bonds used in plastic encapsulated microcircuits (PEMs) has been gradual for the last few years. Many agencies and critics question the long-term robustness and reliability of copper wire bonds; however, PEM manufacturers have been thoroughly evaluating and optimizing the copper wire bond process over the last few years to produce highly reliable products. When the manufacturing process is optimized and closely controlled and monitored, copper wire bonds can be a reliable and stronger alternate to gold wire bonds. This paper details a copper wire bond study that specifically compares copper wire against gold wire bonds following combined environmental life stress test conditions. In this study, bond pull testing indicates good performance of both copper and gold wire bonds. The methods used in this analysis (destructive physical analysis, wire pull testing, and environmental testing) were effective techniques for evaluation of bond wire integrity, detection of manufacturing defects, and overall assessment of copper and gold wire bond processes. Based on wire bond testing in this analysis, the reliability and integrity of optimally developed copper wire bonds appear to be superior to or even stronger than gold wires in the same package type. Equivalent reliability was demonstrated for both copper and gold wire bonds in this test.
A Resolution of the Variability Discrepancy Between Peak Ground Acceleration and Spectral Stress Drop
Authors: Shimmoto Shota 
Published: October 2024

Abstract
It has been recognized that the between-event variability of peak ground acceleration (PGA) is significantly smaller than the variability of the stress drop calculated from corner frequency (the spectral stress drop). Resolving this discrepancy is indispensable for improving seismic hazard assessment because the spectral stress drop is considered a fundamental parameter for predicting high-frequency ground motions. This study addresses this paradox for Mw 3.6–7.1 crustal earthquakes in Japan. Two factors are essential for resolving the problem: (1) calculating the spectral stress drop using a high-frequency-fitted corner frequency (called the stress parameter Δσfch) and (2) considering the magnitude dependence of Δσfch. To estimate Δσfch, the source spectra for crustal earthquakes in Japan are obtained using the two-stage spectral ratio method, which enables the estimation of double-corner-frequency spectra. This two-stage approach is more effective for accurately estimating Δσfch than the standard spectral ratio approach that assumes the single-corner frequency model. This study shows that Δσfch increases with increasing magnitude up to Mw∼5.5 and then becomes constant. The variability of Δσfch calculated by considering this magnitude dependence of Δσfch aligns with the between-event variability of PGA. Incorporating a double-corner-frequency model is crucial for predicting ground-motion variability and enhancing seismic hazard assessments.