bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of implantable cardioverter-defibrillator in patients with diabetes and non-ischaemic systolic heart failure
Authors: Rørth Rasmus , Thune Jens Jakob , Nielsen Jens C , Haarbo Jens , Videbæk Lars , Korup Eva , Signorovitch James , Bruun Niels E , Eiskjær Hans , Hassager Christian , Svendsen Jesper Hastrup , Høfsten Dan E , Torp-Pedersen Christian , Pehrson Steen , Køber Lars , Kristensen Søren L 
Published: April 2019

Abstract

Aims
Implantable cardioverter-defibrillator (ICD) implantation reduce the risk of sudden cardiac death, but not all-cause death in patients with non-ischaemic systolic heart failure (HF). Whether co-existence of diabetes affects ICD treatment effects is unclear.

Methods and results
We examined the effect of ICD implantation on risk of all-cause death, cardiovascular death, and sudden cardiac death (SCD) according to diabetes status at baseline in the Danish Study to Assess the Efficacy of ICDs in Patients with Non-ischaemic Systolic Heart Failure on Mortality (DANISH) trial. Outcomes were analysed by use of cumulative incidence curves and Cox regressions models. Of the 1116 patients enrolled, 211 (19%) had diabetes at baseline. Patients with diabetes were more obese, had worse kidney function and more were in New York Heart Association Class III/IV. The risk of device infections and other complications in the ICD group was similar among patients with and without diabetes (6.1% vs. 4.6% P = 0.54). Irrespective of treatment group, diabetes was associated with higher risk of all-cause death, cardiovascular death, and SCD. The treatment effect of ICD in patients with diabetes vs. patients without diabetes was hazard ratio (HR) = 0.92 (0.57–1.50) vs. HR = 0.85 (0.63–1.13); Pinteraction = 0.60 for all-cause mortality, HR = 0.99 (0.58–1.70) vs. HR = 0.70 (0.48–1.01); Pinteraction = 0.25 for cardiovascular death, and HR = 0.81 (0.35–1.88) vs. HR = 0.40 (0.22–0.76); Pinteraction = 0.16 for sudden cardiac death.

Conclusion
Among patients with non-ischaemic systolic HF, diabetes was associated with higher incidence of all-cause mortality, primarily driven by cardiovascular mortality including SCD. Treatment effect of ICD therapy was not significantly modified by diabetes which might be due to lack of power.
A cellular model of Brugada syndrome with SCN10A variants using human-induced pluripotent stem cell-derived cardiomyocytes
Authors: El-Battrawy Ibrahim , Albers Sebastian , Cyganek Lukas , Zhao Zhihan , Lan Huan , Li Xin , Xu Qiang , Kleinsorge Mandy , Huang Mengying , Liao Zhenxing , Zhong Rujia , Rudic Boris , Müller Jonas , Dinkel Hendrik , Lang Siegfried , Diecke Sebastian , Zimmermann Wolfram-Hubertus , Utikal Jochen , Wieland Thomas , Borggrefe Martin , Zhou Xiaobo , Akin Ibrahim 
Published: April 2019

Abstract

Aims
Brugada syndrome (BrS) is associated with a pronounced risk to develop sudden cardiac death (SCD). Up to 21% of patients are related to mutations in SCN5A. Studies identified SCN10A as a contributor of BrS. However, the investigation of the human cellular phenotype of BrS in the presence of SCN10A mutations remains lacking. The objective of this study was to establish a cellular model of BrS in presence of SCN10A mutations using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).

Methods and results
Dermal fibroblasts obtained from a BrS patient suffering from SCD harbouring the SCN10A double variants (c.3803G>A and c.3749G>A) and three independent healthy control subjects were reprogrammed to hiPSCs. Human-induced pluripotent stem cells were differentiated into cardiomyocytes (hiPSC-CMs).The hiPSC-CMs from the BrS patient showed a significantly reduced peak sodium channel current (INa) and a significantly reduced ATX II (sea anemone toxin, an enhancer of late INa) sensitive as well as A-887826 (a blocker of SCN10A channel) sensitive late sodium channel current (INa) when compared with the healthy control hiPSC-CMs, indicating loss-of-function of sodium channels. Consistent with reduced INa the action potential amplitude and upstroke velocity (Vmax) were significantly reduced, which may contribute to arrhythmogenesis of BrS. Moreover, Ajmaline effects on action potentials were stronger in BrS-hiPSC-CMs than in healthy control cells. This is in agreement with the higher susceptibility of patients to sodium channel blocking drugs in unmasking BrS.

Conclusion
Patient-specific hiPSC-CMs are able to recapitulate single-cell phenotype features of BrS with SCN10A mutations and may provide novel opportunities to further elucidate the cellular disease mechanism.
Chromosome-scale genome assembly of kiwifruit Actinidia eriantha with single-molecule sequencing and chromatin interaction mapping
Authors: Tang Wei , Sun Xuepeng , Yue Junyang , Tang Xiaofeng , Jiao Chen , Yang Ying , Niu Xiangli , Miao Min , Zhang Danfeng , Huang Shengxiong , Shi Wei , Li Mingzhang , Fang Congbing , Fei Zhangjun , Liu Yongsheng 
Published: April 2019

Abstract

Background
Kiwifruit (Actinidia spp.) is a dioecious plant with fruits containing abundant vitamin C and minerals. A handful of kiwifruit species have been domesticated, among which Actinidiaeriantha is increasingly favored in breeding owing to its superior commercial traits. Recently, elite cultivars from A. eriantha have been successfully selected and further studies on their biology and breeding potential require genomic information, which is currently unavailable.

Findings
We assembled a chromosome-scale genome sequence of A. eriantha cultivar White using single-molecular sequencing and chromatin interaction map–based scaffolding. The assembly has a total size of 690.6 megabases and an N50 of 21.7 megabases. Approximately 99% of the assembly were in 29 pseudomolecules corresponding to the 29 kiwifruit chromosomes. Forty-three percent of the A. eriantha genome are repetitive sequences, and the non-repetitive part encodes 42,988 protein-coding genes, of which 39,075 have homologues from other plant species or protein domains. The divergence time between A. eriantha and its close relative Actinidia chinensis is estimated to be 3.3 million years, and after diversification, 1,727 and 1,506 gene families are expanded and contracted in A. eriantha, respectively.

Conclusions
We provide a high-quality reference genome for kiwifruit A. eriantha. This chromosome-scale genome assembly is substantially better than 2 published kiwifruit assemblies from A. chinensis in terms of genome contiguity and completeness. The availability of the A. eriantha genome provides a valuable resource for facilitating kiwifruit breeding and studies of kiwifruit biology.
Estimating age composition for multiple years when there are gaps in the ageing data: the case of western Atlantic bluefin tuna
Authors: Ailloud Lisa E , Lauretta Matthew V , Walter John F , Hoenig John M 
Published: April 2019

Abstract
Age–length key (ALK) methods generally perform well when length samples and age samples are representative of the underlying population. It is unclear how well these methods perform when lengths are representative but age samples are sparse (i.e. age samples are small or missing in many years, and some length groups do not have any age observations). With western Atlantic bluefin tuna, the available age data are sparse and have been, for the most part, collected opportunistically. We evaluated two methods capable of accommodating sparse age data: a novel hybrid ALK (combining forward ALKs and cohort slicing) and the combined forward-inverse ALK. Our goal was to determine if the methods performed better than cohort slicing, which has traditionally been used to obtain catch-at-age for Atlantic bluefin tuna, given the data limitations outlined above. Simulation results indicated that the combined forward-inverse ALK performed much better than the other methods. When applied to western Atlantic bluefin tuna data, the combined forward-inverse ALK approach was able to track cohorts and identified an inconsistency in the ageing of some samples.
Early Measles Vaccination During an Outbreak in the Netherlands: Short-Term and Long-Term Decreases in Antibody Responses Among Children Vaccinated Before 12 Months of Age
Authors: Brinkman Iris D , de Wit Jelle , Smits Gaby P , ten Hulscher Hinke I , Jongerius Maria C , Abreu Taymara C , van der Klis Fiona R M , Hahné Susan J M , Koopmans Marion P G , Rots Nynke Y , van Baarle Debbie , van Binnendijk Robert S 
Published: April 2019

AbstractBackgroundThe majority of infants will not be protected by maternal antibodies until their first measles vaccination, between 12 and 15 months of age. This provides incentive to reduce the age at measles vaccination, but immunological consequences are insufficiently understood, and long-term effects are largely unknown.MethodsA total of 79 infants who received early measles vaccination between 6 and 12 months age and a second dose at 14 months of age were compared to 44 children in a control group who received 1 dose at 14 months of age. Measles virus–specific neutralizing antibody concentrations and avidity were determined up to 4 years of age.ResultsInfants who first received measles vaccination before 12 months of age had a long-term decrease in the concentration and avidity of measles virus–specific neutralizing antibodies, compared with infants in the control group. For 11.1% of children with a first dose before 9 months of age, antibody levels at 4 years of age had dropped below the cutoff for clinical protection.ConclusionsEarly measles vaccination provides immediate protection in the majority of infants but yields a long-term decrease in neutralizing antibody responses, compared to vaccination at a later age. Additional vaccination at 14 months of age does not improve this. Over the long term, this may result in an increasing number of children susceptible to measles.
Intravenous colistin use for infections due to MDR Gram-negative bacilli in critically ill paediatric patients: a systematic review and meta-analysis
Authors: Karageorgos Spyridon A , Bassiri Hamid , Siakallis George , Miligkos Michael , Tsioutis Constantinos 
Published: April 2019

Abstract

Background
Data are limited regarding the clinical effectiveness and safety of intravenous colistin for treatment of infections due to MDR Gram-negative bacilli (GNB) in paediatric ICUs (PICUs).

Methods
Systematic review of intravenous colistin use in critically ill paediatric patients with MDR-GNB infection in PubMed, Scopus and EMBASE (up to 31 January 2018).

Results
Out of 1181 citations, 7 studies were included on the use of intravenous colistin for 405 patients in PICUs. The majority of patients were diagnosed with lower respiratory tract infections, Acinetobacter baumannii being the predominant pathogen. Colistin dosages ranged between 2.6 and 18 mg/kg/day, with only one case reporting a loading dose. Emergence of colistin resistance during treatment was reported in two cases. Nephrotoxicity and neurotoxicity were reported in 6.1% and 0.5%, respectively, but concomitant medications and severe underlying illness limited our ability to definitively associate use of colistin with nephrotoxicity. Crude mortality was 29.5% (95% CI = 21.7%–38.1%), whereas infection-related mortality was 16.6% (95% CI = 12.2%–21.5%).

Conclusions
While the reported incidence of adverse events related to colistin was low, reported mortality rates for infections due to MDR-GNB in PICUs were notable. In addition to severity of disease and comorbidities, inadequate daily dosage and the absence of a loading dose may have contributed to mortality. As the use of colistin for treatment of MDR-GNB infections increases, it is imperative to understand whether optimal dosing of colistin in paediatric patients differs across different age groups. Thus, future studies to establish the pharmacokinetic properties of colistin in different paediatric settings are warranted.
SlSWEET1a is involved in glucose import to young leaves in tomato plants
Authors: Ho Li-Hsuan , Klemens Patrick A W , Neuhaus H Ekkehard , Ko Han-Yu , Hsieh Shu-Ying , Guo Woei-Jiun 
Published: April 2019

Abstract
Sugar allocation from source to sink (young) leaves, critical for plant development, relies on activities of plasma membrane sugar transporters. However, the key sugar unloading mechanism to sink leaves remains elusive. SWEET transporters mediate sugar efflux into reproductive sinks; therefore, they are promising candidates for sugar unloading during leaf growth. Transcripts of SlSWEET1a, belonging to clade I of the SWEET family, were markedly more abundant than those of all other 30 SlSWEET genes in young leaves of tomatoes. High expression of SlSWEET1a was also detected in reproductive sinks, such as flowers. SlSWEET1a was dominantly expressed in leaf unloading veins, and the green fluorescent protein (GFP) fusion protein was localized to the plasma membrane using Arabidopsis protoplasts, further implicating this carrier in sugar unloading. In addition, yeast growth assays and radiotracer uptake analyses further demonstrated that SlSWEET1a acted as a low-affinity (Km ~100 mM) glucose-specific carrier with a passive diffusion manner. Finally, virus-induced gene silencing of SlSWEET1a expression reduced hexose accumulation to ~50% in young leaves, with a parallel 2-fold increase in mature leaves. Thus, we propose a novel function for SlSWEET1a in the uptake of glucose into unloading cells as part of the sugar unloading mechanism in sink leaves of tomato.
Arthroscopy for Management of Femoroacetabular Impingement Syndrome in the Military Health System: A 10-Year Epidemiological Overview of Cases with 2-year Follow-up
Authors: Rhon Daniel , Schmitz Matthew , Mayhew Rachel , Dry Katie , Greenlee Tina 
Published: April 2019

Abstract

Introduction
With the rapid rise in arthroscopy rates for the management of Femoroacetabular Impingement (FAI) Syndrome, it is important to understand current surgical rates and the impact of these surgeries within the Military Health System (MHS). The purpose of this study was to provide an epidemiological descriptive summary of hip arthroscopy for FAI Syndrome in the MHS and describe perioperative healthcare utilization variables.

Methods
Eligible beneficiaries ages 18–50, undergoing hip arthroscopy with 2-year follow-up after surgery were included. Healthcare utilization data were abstracted from the MHS Data Repository (MDR) from June 2003 to July 2015, and included all visits, costs, procedures, and prescriptions taking place in both military and civilian hospitals worldwide.

Results
1870 patients were included (mean age 32.2 years; 55.5% male). 51.7% of the procedures took place in military versus 48.3% in civilian hospitals. Mean hip-related healthcare costs in the 2-year following surgery were $15,434 per patient. Patients had a median of 3 opioid prescriptions and 72% had a comorbidity present after surgery. Generally, rates of surgery grew annually from 66 cases in 2004 to 422 cases in 2013. Overall complication rates were comparable to other published reports. Procedures in both military and civilian hospitals had the same rates of femoroplasty and labral repairs, however acetabuloplasty procedures occurred at a higher rate in military (18.9%) vs civilian (14.7%) hospitals. Only 58.8% of patients had physical therapy in the year prior to surgery, while 82.7% had it after surgery. Additionally, 50% of patients had received opioid prescriptions in the 1 year prior to surgery, while 38.9% had 3 or greater opioid prescriptions beyond the initial perioperative fill within the 2-year follow-up.

Conclusion
Rates of arthroscopy have grown in the MHS over the last decade. Complication rates are similar to those reported in other populations and settings. Utilization of physical therapy was much more likely after surgery than prior to it. Opioid use was high prior to surgery and many individuals continued to receive opioid prescriptions beyond the initial perioperative period.
The GREATS H β + [O iii] luminosity function and galaxy properties at z ∼ 8: walking the way of JWST
Authors: De Barros S , Oesch P A , Labbé I , Stefanon M , González V , Smit R , Bouwens R J , Illingworth G D 
Published: April 2019

ABSTRACT
The James Webb Space Telescope will allow to spectroscopically study an unprecedented number of galaxies deep into the reionization era, notably by detecting [O iii]λλ4959, 5007, and H β nebular emission lines. To efficiently prepare such observations, we photometrically select a large sample of galaxies at z ∼ 8 and study their rest-frame optical emission lines. Combining data from the GOODS Re-ionization Era wide-Area Treasury from Spitzer (GREATS) survey and from HST we perform spectral energy distribution (SED) fitting, using synthetic SEDs from a large grid of photoionization models. The deep Spitzer/IRAC data combined with our models exploring a large parameter space enables to constrain the [O iii] + H β fluxes and equivalent widths for our sample, as well as the average physical properties of z ∼ 8 galaxies, such as the ionizing photon production efficiency with $\log (\xi _\mathrm{ion}/\mathrm{erg}^{-1}\mathrm{Hz})\ge 25.77$. We find a relatively tight correlation between the [O iii] + H β and UV luminosity, which we use to derive for the first time the [O iii]λλ4959, 5007 + H β luminosity function (LF) at z ∼ 8. The z ∼ 8 [O iii] + H β LF is higher at all luminosities compared to lower redshift, as opposed to the UV LF, due to an increase of the [O iii] + H β luminosity at a given UV luminosity from z ∼ 3 to z ∼ 8. Finally, using the [O iii] + H β LF, we make predictions for JWST/NIRSpec number counts of z ∼ 8 galaxies. We find that the current wide-area extragalactic legacy fields are too shallow to use JWST at maximal efficiency for z ∼ 8 spectroscopy even at 1 h depth and JWST pre-imaging to ≳30 mag will be required.
Temporal correlation between the optical and γ-ray flux variations in the blazar 3C 454.3
Authors: Rajput Bhoomika , Stalin C S , Sahayanathan S , Rakshit Suvendu , Mandal Amit Kumar 
Published: April 2019

ABSTRACT
Blazars show optical and γ-ray flux variations that are generally correlated, although there are exceptions. Here we present anomalous behaviour seen in the blazar 3C 454.3 based on an analysis of quasi-simultaneous data at optical, ultraviolet, X-ray, and γ-ray energies, spanning about 9 yr from 2008 August to 2017 February. We have identified four time intervals (epochs), A, B, D, and E, when the source showed large-amplitude optical flares. In epochs A and B the optical and γ-ray flares are correlated, while in D and E corresponding flares in γ-rays are weak or absent. In epoch B the degree of optical polarization strongly correlates with changes in optical flux during a short-duration optical flare superimposed on one of long duration. In epoch E the optical flux and degree of polarization are anticorrelated during both the rising and declining phases of the optical flare. We carried out broad-band spectral energy distribution (SED) modelling of the source for the flaring epochs A,B, D, and E, and a quiescent epoch, C. Our SED modelling indicates that optical flares with absent or weak corresponding γ-ray flares in epochs D and E could arise from changes in a combination of parameters, such as the bulk Lorentz factor, magnetic field, and electron energy density, or be due to changes in the location of the γ-ray-emitting regions.
Feasibility of Robotic-Assisted Neurovascular Interventions: Initial Experience in Flow Model and Porcine Model
Authors: Britz Gavin W , Tomas Johnny , Lumsden Alan 
Published: April 2019

Abstract

BACKGROUND
Despite advances in robotic-assisted technology for cardiac and peripheral vascular interventions, a robotic-assisted platform for neurovascular intervention is not yet available.

OBJECTIVE
To evaluate the feasibility of the CorPath® GRX robotic-assisted platform (Corindus Inc, Waltham, Massachusetts) for neurovascular interventions through preclinical study.

METHODS
The robotic system was tested for its ability to accurately navigate a variety of common neurovascular devices in an in Vitro flow model and in a live, anesthetized pig, under conditions and following procedures appropriate for clinical intervention. An access catheter was introduced manually at the equivalent of the common carotid artery in both models. Endovascular wires and catheters were navigated through the external and internal carotid artery and posterior cerebral vasculature under robotic assistance, using 0.014 in guidewires, 2.4F/1.7F microcatheters, bare-metal stents, and embolic coils.

RESULTS
All procedures in both the flow and porcine models, including navigation, wiring, and deployment of the stent and coils, were performed successfully with no technical complications. There was no evidence of extravasation, dissection, thrombosis, or other vascular injury when angiography was compared before and after the live-animal procedure.

CONCLUSION
This is the first study to demonstrate that use of a robotic-assisted platform is feasible for intracranial neurovascular intervention. The robotic system was successful at navigating and deploying the small-gauge devices specific to neurovascular procedures. Given the potential benefits of robotic-assisted surgery for the patient and the surgeon, further investigation is warranted for this indication.
Ionization age of iron ejecta in the Galactic Type Ia supernova remnant G306.3−0.9
Authors: Sawada Makoto , Tachibana Katsuhiro , Uchida Hiroyuki , Ito Yuta , Matsumura Hideaki , Bamba Aya , Tsuru Takeshi Go , Tanaka Takaaki 
Published: April 2019

AbstractWe present a 190 ks observation of the Galactic supernova remnant (SNR) G306.3−0.9 with Suzaku. To study ejecta properties of this possible Type Ia SNR, the absolute energy-scale at the Fe-K band was calibrated to a level of uncertainty less than 10 eV by a cross-calibration with the Hitomi microcalorimeter using the Perseus cluster spectra. This enabled us for the first time to determine the ionization state of the Fe Kα line of this SNR accurately. The ionization time-scale (τ) of the Fe ejecta was measured to be log10τ (cm−3 s) $= 10.24\, \pm \, 0.03$, significantly smaller than previous measurements. Marginally detected Kα lines of Cr and Mn have ionization time-scales consistent with that of Fe. The global spectrum was well-fitted with shocked interstellar matter (ISM) and at least two ejecta components with different ionization time-scales for Fe and intermediate mass elements (IME) such as S and Ar. One plausible interpretation of the one-order-of-magnitude shorter time-scale of Fe than that of IME ($\log _{10} \tau = 11.17\, \pm \, 0.07$) is a chemically stratified structure of ejecta. By comparing the X-ray absorption column to the H i distribution decomposed along the line of sight, we refined the distance to ∼20 kpc. The large ISM-to-ejecta shocked mass ratio of ∼100 and dynamical time-scale of ∼6 kyr place the SNR in the late Sedov phase. These properties are consistent with a stratified ejecta structure that has survived the mixing processes expected in an evolved SNR.