bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New Flexible Multibody Dynamics Analysis Methodology of Deployable Structures with Scissor-Like Elements
Authors: Peng Qi’an , Wang Sanmin , Zhi Changjian , Li Bo 
Published: September 2019

Abstract
There are vast constraint equations in conventional dynamics analysis of deployable structures, which lead to differential-algebraic equations (DAEs) solved hard. To reduce the difficulty of solving and the amount of equations, a new flexible multibody dynamics analysis methodology of deployable structures with scissor-like elements (SLEs) is presented. Firstly, a precise model of a flexible bar of SLE is established by the higher order shear deformable beam element based on the absolute nodal coordinate formulation (ANCF), and the master/slave freedom method is used to obtain the dynamics equations of SLEs without constraint equations. Secondly, according to features of deployable structures, the specification matrix method (SMM) is proposed to eliminate the constraint equations among SLEs in the frame of ANCF. With this method, the inner and the boundary nodal coordinates of element characteristic matrices can be separated simply and efficiently, especially on condition that there are vast nodal coordinates. So the element characteristic matrices can be added end to end circularly. Thus, the dynamic model of deployable structure reduces dimension and can be assembled without any constraint equation. Next, a new iteration procedure for the generalized-α algorithm is presented to solve the ordinary differential equations (ODEs) of deployable structure. Finally, the proposed methodology is used to analyze the flexible multi-body dynamics of a planar linear array deployable structure based on three scissor-like elements. The simulation results show that flexibility has a significant influence on the deployment motion of the deployable structure. The proposed methodology indeed reduce the difficulty of solving and the amount of equations by eliminating redundant degrees of freedom and the constraint equations in scissor-like elements and among scissor-like elements.
An exclusion experiment to study the influence of habitat structure provided by Mussismilia corals (Cnidaria; Anthozoa) on the predation of associated crustaceans
Authors: Nogueira Marcos M. , Neves Elizabeth , Johnsson Rodrigo 
Published: September 2019

Abstract
Predation is an interaction between species that influences community organisation by the direct consumption of prey, influencing prey numbers, behaviours and traits. The intensity of predation is greatly influenced by the environment, and the physical structure of habitats may influence predation intensity by providing refuge for prey or reducing the foraging efficiency of predators. In this context, the aim of the present study is to verify the influence of predation on the richness and density patterns of crustaceans inhabiting different species of Mussismilia corals, which provide various habitat structures for the associated fauna. We conducted an exclusion experiment to include total, partial and no cage treatments. The richness and density of crustaceans differed among coral species and cage treatments, except between partial cage and no cage treatment. Mussismilia harttii showed higher richness and density in uncaged and partial cage treatments compared with M. braziliensis, which in turn showed higher values than M. hispida. These findings indicate the importance of predation in the structure of crustacean assemblage associated with Mussismilia species and that differences in the richness and abundance of associated fauna result from the different habitat structures provided by each species of Mussismilia.
Prediction of fecal indicator organism concentrations in rivers: the shifting role of environmental factors under varying flow conditions
Authors: Herrig Ilona , Seis Wolfgang , Fischer Helmut , Regnery Julia , Manz Werner , Reifferscheid Georg , Böer Simone 
Published: September 2019

Abstract

Background
Fecal indicator organisms such as Escherichia coli, enterococci, and coliphages are important to assess, monitor, and predict microbial water quality in natural freshwater ecosystems. To improve predictive modelling of fecal indicators in surface waters, it is vital to assess the influence of autochthonous and allochthonous environmental factors on microbial water quality in riverine systems. To better understand how environmental conditions influence the fate of fecal indicators under varying weather conditions, the interdependencies of environmental parameters and concentrations of E. coli, intestinal enterococci, and somatic coliphages were studied at two rivers (Rhine and Moselle in Rhineland-Palatinate, Germany) over a period of 2 years that exhibited contrasting hydrological conditions. Both riverine sampling sites were subject to similar meteorological conditions based on spatial proximity, but differed in hydrodynamics and hydrochemistry, thus providing further insight into the role of river-specific determinants on fecal indicator concentrations. Furthermore, a Bayesian multiple linear regression approach that complies with the European Bathing Water Directive was applied to both rivers’ datasets to test model transferability and the validity of microbial water quality predictions in riverine systems under varying flow regimes.

Results
According to multivariate statistical analyses, rainfall events and high water discharge favored the input and dissemination of fecal indicators in both rivers. As expected, concentrations declined with rising global solar irradiance, water temperature, and pH. While variations in coliphage concentrations were predominantly driven by hydro-meteorological factors, bacterial indicator concentrations were strongly influenced by autochthonous biotic factors related to primary production. This was more pronounced under low flow conditions accompanied by strong phytoplankton blooms. Strong seasonal variations pointed towards bacterial indicator losses due to grazing activities. The Bayesian linear regression approach provided appropriate water quality predictions at the Rhine sampling site based on discharge, global solar irradiance, and rainfall as fecal indicator distributions were predominantly driven by hydro-meteorological factors.

Conclusions
Assessment of microbial water quality predictions implied that rivers characterized by strong hydrodynamics qualify for multiple linear regression models using readily measurable hydro-meteorological parameters. In rivers where trophic interactions exceed hydrodynamic influences, such as the Moselle, viral indicators may pose a more reliable response variable in statistical models.
Overlapping transcriptional expression response of wheat zinc-induced facilitator-like transporters emphasize important role during Fe and Zn stress
Authors: Sharma Shivani , Kaur Gazaldeep , Kumar Anil , Meena Varsha , Kaur Jaspreet , Pandey Ajay Kumar 
Published: September 2019

Abstract

Background
Hexaploid wheat is an important cereal crop that has been targeted to enhance grain micronutrient content including zinc (Zn) and iron (Fe). In this direction, modulating the expression of plant transporters involved in Fe and Zn homeostasis has proven to be one of the promising approaches. The present work was undertaken to identify wheat zinc-induced facilitator-like (ZIFL) family of transporters. The wheat ZIFL genes were characterized for their transcriptional expression response during micronutrient fluctuations and exposure to multiple heavy metals.

Results
The genome-wide analyses resulted in identification of fifteen putative TaZIFL-like genes, which were distributed only on Chromosome 3, 4 and 5. Wheat ZIFL proteins subjected to the phylogenetic analysis showed the uniform distribution along with rice, Arabidopsis and maize. In-silico analysis of the promoters of the wheat ZIFL genes demonstrated the presence of multiple metal binding sites including those which are involved in Fe and heavy metal homeostasis. Quantitative real-time PCR analysis of wheat ZIFL genes suggested the differential regulation of the transcripts in both roots and shoots under Zn surplus and also during Fe deficiency. Specifically, in roots, TaZIFL2.3, TaZIFL4.1, TaZIFL4.2, TaZIFL5, TaZIFL6.1 and TaZIFL6.2 were significantly up-regulated by both Zn and Fe. This suggested that ZIFL could possibly be regulated by both the nutrient stress in a tissue specific manner. When exposed to heavy metals, TaZIFL4.2 and TaZIFL7.1 show significant up-regulation, whereas TaZIFL5 and TaZIFL6.2 remained almost unaffected.

Conclusion
This is the first report for detailed analysis of wheat ZIFL genes. ZIFL genes also encode for transporter of mugineic acid (TOM) proteins, that are involved in the release of phytosiderophores to enhance Fe/Zn uptake. The detailed expression analysis suggests the varying expression patterns during development of wheat seedlings and also against abiotic/biotic stresses. Overall, this study will lay foundation to prioritize functional assessment of the candidate ZIFL as a putative TOM protein in wheat.
Mouse corticospinal system comprises different functional neuronal ensembles depending on their hodology
Authors: Olivares-Moreno Rafael , López-Hidalgo Mónica , Altamirano-Espinoza Alain , González-Gallardo Adriana , Antaramian Anaid , Lopez-Virgen Verónica , Rojas-Piloni Gerardo 
Published: September 2019

Abstract

Background
Movement performance depends on the synaptic interactions generated by coherent parallel sensorimotor cortical outputs to different downstream targets. The major outputs of the neocortex to subcortical structures are driven by pyramidal tract neurons (PTNs) located in layer 5B. One of the main targets of PTNs is the spinal cord through the corticospinal (CS) system, which is formed by a complex collection of distinct CS circuits. However, little is known about intracortical synaptic interactions that originate CS commands and how different populations of CS neurons are functionally organized. To further understand the functional organization of the CS system, we analyzed the activity of unambiguously identified CS neurons projecting to different zones of the same spinal cord segment using two-photon calcium imaging and retrograde neuronal tracers.

Results
Sensorimotor cortex slices obtained from transgenic mice expressing GCaMP6 funder the Thy1 promoter were used to analyze the spontaneous calcium transients in layer 5 pyramidal neurons. Distinct subgroups of CS neurons projecting to dorsal horn and ventral areas of the same segment show more synchronous activity between them than with other subgroups.

Conclusions
The results indicate that CS neurons projecting to different spinal cord zones segregated into functional ensembles depending on their hodology, suggesting that a modular organization of CS outputs controls sensorimotor behaviors in a coordinated manner.
Four-color multiplex real-time PCR assay prototype targeting azithromycin resistance mutations in Mycoplasma genitalium
Authors: Thellin Olivier , Elmoualij Benaïssa , Zorzi Willy , Jensen Jorgen S. , Close Renaud , Deregowski Valerie , Le Guern Fellous Muriel , Quatresooz Pascale 
Published: September 2019

Abstract

Background
The worldwide expansion of macrolide-resistant Mycoplasma genitalium (MG) in cases of genital infections has led to an increased recurrence rate of these infections after first-line azithromycin treatment. By detecting the presence of azithromycin-resistant MG, the patient’s antibiotic treatment can be targeted and the spread of resistance prevented. With this aim in mind, macrolide-resistance detection kits are helpful tools for the physician.

Methods
Azithromycin resistance mutations in MG are targeted using a four-color multiplex real-time RT-PCR assay. Tested targets include plasmid DNA (as positive controls) as well as macrolide-sensitive and macrolide-resistant genomic DNA from characterized cell lines and clinical samples.

Results
The analytical data presented here were generated from plasmid DNA and genomic RNA/DNA and include adaptation to an internal control, specificity between targets, specificity vs non-MG species, limit of detection (LoD) and interference studies (co-infection and endogenous substances). The clinical data were based on the application of the assay to clinical samples characterized by sequencing.

Conclusions
A new NAAT (nucleic acid amplification test) prototype has been developed in collaboration with the Diagenode s.a. company, this prototype targets MG and azithromycin-resistance mutations in that pathogen.
How to develop a sustainable telemedicine service? A Pediatric Telecardiology Service 20 years on - an exploratory study
Authors: Maia Mélanie Raimundo , Castela Eduardo , Pires António , Lapão Luís Velez 
Published: September 2019

Abstract

Background
Telemedicine services are promoting more access to healthcare. Portugal was an early adopter of telemedicine to overcome both its geological barriers and the shortage of healthcare professionals. The Pediatric Cardiology Service (PCS) at Coimbra University Hospital Centre (CHUC) has been using telemedicine to increase access and coverage since 1998. Their Pediatric Telecardiology Service has been daily connecting CHUC with 13 other Portuguese national hospitals, and regularly connecting with Portuguese-speaking African countries, through a teleconsultation platform.

Methods
This study aims at exploring the Pediatric Telecardiology Service’s evolution, through a comprehensive assessment of the PCS’s development, evolution and impact in public health, to better understand the critical factors for implementation and sustainability of telemedicine, in the context of healthcare services digitalization. A case study was performed, with cost-benefit, critical factors and organizational culture assessment. Finally, the Kingdon’s framework helped to understand the implementation and scale-up process and the role of policy-making.

Results
With the total of 32,685 out-patient teleconsultations, growing steadily from 1998 to 2016, the Pediatric Telecardiology Service has reached national and international recognition, being a pioneer and an active promotor of telemedicine. This telemedicine service has saved significant resources, about 1.1 million euros for the health system (e.g. in administrative and logistic costs) and approximately 419 euros per patient (considering an average of 1777 patients per year).
PCS presents a dominant “Clan” culture. The Momentum’s critical factors for telemedicine service implementation enabled us to understand how barriers were overcome (e.g. political forces). Willingness, perseverance and teamwork, allied with partnership with key stakeholders, were the foundation for professionals’ engagement and service networking development. Its positive results, new regulations and the increasing support from the hospital board, set up a window of opportunity to establish a sustainable telemedicine service.

Conclusion
The Pediatric Telecardiology Service enables real-time communication and the sharing of clinical information, overcoming many barriers (from geographical ones to shortage of healthcare professionals), improving access to specialized care both in Portugal and Africa.
Motivation and teamwork, and perseverance, were key for the Pediatric Telecardiology Service to tackle the window of opportunity which created conditions for sustainability.
Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory
Authors: Zhang Chuanbo , Li Man , Zhao Guang-Rong , Lu Wenyu 
Published: September 2019

Abstract

Background
Alpha-Terpineol (α-Terpineol), a C10 monoterpenoid alcohol, is widely used in the cosmetic and pharmaceutical industries. Construction Saccharomyces cerevisiae cell factories for producing monoterpenes offers a promising means to substitute chemical synthesis or phytoextraction.

Results
α-Terpineol was produced by expressing the truncated α-Terpineol synthase (tVvTS) from Vitis vinifera in S. cerevisiae. The α-Terpineol titer was increased to 0.83 mg/L with overexpression of the rate-limiting genes tHMG1, IDI1 and ERG20F96W-N127W. A GSGSGSGSGS linker was applied to fuse ERG20F96W-N127W with tVvTS, and expressing the fusion protein increased the α-Terpineol production by 2.87-fold to 2.39 mg/L when compared with the parental strain. In addition, we found that farnesyl diphosphate (FPP) accumulation by down-regulation of ERG9 expression and deletion of LPP1 and DPP1 did not improve α-Terpineol production. Therefore, ERG9 was overexpressed and the α-Terpineol titer was further increased to 3.32 mg/L. The best α-Terpineol producing strain LCB08 was then used for batch and fed-batch fermentation in a 5 L bioreactor, and the production of α-Terpineol was ultimately improved to 21.88 mg/L.

Conclusions
An efficient α-Terpineol production cell factory was constructed by engineering the S. cerevisiae mevalonate pathway, and the metabolic engineering strategies could also be applied to produce other valuable monoterpene compounds in yeast.
A signature of hypoxia-related factors reveals functional dysregulation and robustly predicts clinical outcomes in stage I/II colorectal cancer patients
Authors: Zou Yi-feng , Rong Yu-ming , Tan Ying-xin , Xiao Jian , Yu Zhao-liang , Chen Yu-feng , Ke Jia , Li Cheng-hang , Chen Xi , Wu Xiao-jian , Lan Ping , Lin Xu-tao , Gao Feng 
Published: September 2019

Abstract

Background
The hypoxic tumor microenvironment accelerates the invasion and migration of colorectal cancer (CRC) cells. The aim of this study was to develop and validate a hypoxia gene signature for predicting the outcome in stage I/II CRC patients that have limited therapeutic options.

Methods
The hypoxic gene signature (HGS) was constructed using transcriptomic data of 309 CRC patients with complete clinical information from the CIT microarray dataset. A total of 1877 CRC patients with complete prognostic information in six independent datasets were divided into a training cohort and two validation cohorts. Univariate and multivariate analyses were conducted to evaluate the prognostic value of HGS.

Results
The HGS consisted of 14 genes, and demarcated the CRC patients into the high- and low-risk groups. In all three cohorts, patients in the high-risk group had significantly worse disease free survival (DFS) compared with those in the low risk group (training cohort—HR = 4.35, 95% CI 2.30–8.23, P < 0.001; TCGA cohort—HR = 2.14, 95% CI 1.09–4.21, P = 0.024; meta-validation cohort—HR = 1.91, 95% CI 1.08–3.39, P = 0.024). Compared to Oncotype DX, HGS showed superior predictive outcome in the training cohort (C-index, 0.80 vs 0.65) and the validation cohort (C-index, 0.70 vs 0.61). Pathway analysis of the high- and low-HGS groups showed significant differences in the expression of genes involved in mTROC1, G2-M, mitosis, oxidative phosphorylation, MYC and PI3K–AKT–mTOR pathways (P < 0.005).

Conclusion
Hypoxic gene signature is a satisfactory prognostic model for early stage CRC patients, and the exact biological mechanism needs to be validated further.
Evaluation of Plasmodium falciparum MSP10 and its development as a serological tool for the Peruvian Amazon region
Authors: Bendezu Jorge , Villasis Elizabeth , Morales Ruiz Sandra , Garro Katherine , Infante Berónica , Gutierrez-Loli Renzo , Rodríguez Pamela , Fernández-Díaz Manolo , Gamboa Dionicia , Torres Katherine 
Published: September 2019

Abstract

Background
Different antigens are needed to characterize Plasmodium falciparum infection in terms of seroreactivity and targets for invasion inhibition, in order to guide and identify the proper use of such proteins as tools for the development of serological markers and/or as vaccine candidates.

Methods
IgG responses in 84 serum samples from individuals with P. falciparum infection [classified as symptomatic (Sym) or asymptomatic (Asym)], or acute Plasmodium vivax infection, from the Peruvian Amazon region, were evaluated by enzyme-linked immunosorbent assays specific for a baculovirus-produced recombinant protein P. falciparum Merozoite Surface Protein 10 (rMSP10) and for non-EGF region selected peptides of PfMSP10 selected by a bioinformatics tool (PfMSP10-1, PfMSP10-2 and PfMSP10-3). Monoclonal antibodies against the selected peptides were evaluated by western blotting, confocal microscopy and inhibition invasion assays.

Results
Seroreactivity analysis of the P. falciparum Sym- and Asym-infected individuals against rMSP10 showed a higher response as compared to the individuals with P. vivax acute infection. IgG responses against peptide PfMSP10-1 were weak. Interestingly high IgG response was found against peptide PfMSP10-2 and the combination of peptides PfMSP10-1 + PfMSP10-2. Monoclonal antibodies were capable of detecting native PfMSP10 on purified schizonts by western blot and confocal microscopy. A low percentage of inhibition of merozoite invasion of erythrocytes in vitro was observed when the monoclonal antibodies were compared with the control antibody against AMA-1 antigen. Further studies are needed to evaluate the role of PfMSP10 in the merozoite invasion.

Conclusions
The rMSP10 and the PfMSP10-2 peptide synthesized for this study may be useful antigens for evaluation of P. falciparum malaria exposure in Sym and Asym individuals from the Peruvian Amazon region. Moreover, these antigens can be used for further investigation of the role of this protein in other malaria-endemic areas.
Differential expression of var subgroups and PfSir2a genes in afebrile Plasmodium falciparum malaria: a matched case–control study
Authors: Gupta Himanshu , Galatas Beatriz , Matambisso Gloria , Nhamussua Lidia , Cisteró Pau , Bassat Quique , Casellas Aina , Macete Eusébio , Aponte John J. , Sacoor Charfudin , Alonso Pedro , Saúte Francisco , Guinovart Caterina , Aide Pedro , Mayor Alfredo 
Published: September 2019

Abstract

Background
Poor knowledge on the afebrile Plasmodium falciparum biology limits elimination approaches to target asymptomatic malaria. Therefore, the association of parasite factors involved in cytoadhesion, parasite multiplication and gametocyte maturation with afebrile malaria was assessed.

Methods
Plasmodium falciparum isolates were collected from febrile (axillary temperature ≥ 37.5 °C or a reported fever in the previous 24 h) and afebrile (fever neither at the visit nor in the previous 24 h) individuals residing in Southern Mozambique. var, PfSir2a and Pfs25 transcript levels were determined by reverse transcriptase quantitative PCRs (RT-qPCRs) and compared among 61 pairs of isolates matched by parasite density, age and year of sample collection.

Results
The level of varC and PfSir2a transcripts was higher in P. falciparum isolates from afebrile individuals (P ≤ 0.006), while varB and DC8 genes (P ≤ 0.002) were higher in isolates from individuals with febrile infections. After adjusting the analysis by area of residence, doubling the relative transcript unit (RTU) of varC and PfSir2a was associated with a 29.7 (95% CI 4.6–192.3) and 8.5 (95% CI 1.9–32.2) fold increases, respectively, of the odds of being afebrile. In contrast, doubling the RTU of varB and DC8 was associated with a 0.8 (95% CI 0.05–0.6) and 0.2 (95% CI 0.04–0.6) fold changes, respectively, of the odds of being afebrile. No significant differences were found for Pfs25 transcript levels in P. falciparum isolates from afebrile and febrile individuals.

Conclusions
var and gametocyte-specific transcript patterns in febrile and afebrile infections from southern Mozambique matched by age, parasite density and recruitment period suggest similar transmissibility but differential expression of variant antigens involved in cytoadhesion and immune-evasion.