bluuebunny/crossref_metadata_2025_split huggingface dataset:
The relationship betweenInterleukin-27gene polymorphisms and Kawasaki disease in a population of Chinese children
Authors: Si Feifei , Wu Yao , Wang Xianmin , Gao Fang , Yang Dan , Liu Ruixi , Yi Qijian 
Published: July 2018

AbstractBackgroundKawasaki disease is the leading cause of acquired heart disease in children from developed countries. The Interleukin-6/ Interleukin-12 cytokine family has many members, including the paradoxical anti- and pro-inflammatory Interleukin-27. Recent studies have demonstrated that Interleukin-27 plays a role in immune diseases. Given this, we sought to evaluate the association betweenInterleukin-27genetic polymorphisms and Kawasaki disease in Chinese children.Methods and resultsInterleukin-27 was genotyped in 100 Kawasaki disease children and 98 healthy children (controls), resulting in the direct sequencing of eight Single-nucleotide Polymorphisms: rs17855750, rs40837, rs26528, rs428253, rs4740, rs4905, rs153109, and rs181206). There were no significant differences in Interleukin-27 genotypes between Kawasaki disease and control groups. Of the eight Single-nucleotide Polymorphisms, there was a significant increase in the risk of Kawasaki disease with coronary arterial lesions in children with the rs17855750 (T>G), rs40837 (A>G), rs4740 (G>A), rs4905 (A>G), rs153109 (T>C), and rs26528 (A>G) Single-nucleotide Polymorphisms. This was particularly true for rs17855750 (T>G), which had a greater frequency in Kawasaki disease children with coronary arterial aneurysm.ConclusionThese findings may be used as risk factors when assessing a child’s likelihood of developing Kawasaki disease, as well as for the development of future therapeutic treatments for Kawasaki disease.
Life Charting of Affective Disorders
Authors: Leverich Gabriele S. , Post Robert M. 
Published: July 2018

AbstractThe recurrent and frequently chronic course of affective disorders requires careful delineation of the number, frequency, and pattern of prior and current episodes and their response to pharmacotherapies to help develop optimal assessment and treatment approaches for these Potentially lethal medical illnesses. To better track and monitor the longitudinal course of unipolar and bipolar illness and to promote more effective management, we developed the retrospective and prospective National Institute of Mental Health Life Chart Methodology (NIMH-LCM). The principles of retrospective and prospective life charting are the focus of this article. Following introductory background information on affective disorders, the influence of Kraepelin's work and his use of life charts are reviewed as the basis and framework for the NIMH-LCM. The use of life charting both retrospectively and prospectively is discussed, with examples of its utility and benefits.
Psychosocial Approaches to the Course and Treatment of Bipolar Disorder
Authors: Miklowitz David J. 
Published: July 2018

AbstractThe course of bipolar disorder is marked by relapses and remissions, even when patients are maintained on appropriate pharmacotherapy. This variability in illness course may occur in part because the disorder is affected by socioenvironmental stressors, particularly significant life events and high-conflict family relationships. Considering these factors, clinicians have focused more attention on psychosocial treatments as useful adjuncts to pharmacotherapy. In this article, a psychosocial treatment program known as family-focused psychoeducational treatment (FFT) is described. Delivered in combination with pharmacotherapy during the postepisode stabilization period, FFT begins with an assessment of the family or marital environment. Then, in three consecutive, modules, participants receive education about the nature, causes, and treatment of bipolar disorder, communication enhancement training, and problem-solving skills training. The overall goals of the program are to restore family equilibrium and improve the patient's clinical functioning after the acute-illness episode. A case study illustrating the approach is presented, and the future directions of FFT are reviewed.
Jointed goatgrass (Aegilops cylindrica): A Review
Authors: Mallory-Smith Carol , Kniss Andrew R. , Lyon Drew J. , Zemetra Robert S. 
Published: July 2018

AbstractIn 1994, the National Jointed Goatgrass Research Program was initiated with funding from a special USDA grant. The 15-yr program provided $4.1 million to support jointed goatgrass (Aegilops cylindricaHost.) research and technology transfer projects in 10 western states. These projects resulted in approximately 80 refereed manuscripts, including journal articles and extension publications. The research covered various topics related to the biology and ecology of jointed goatgrass as well as its management and control in wheat (Triticum aestivumL.) production systems. This review summarizes the research on jointed goatgrass published after Donald and Ogg’s 1991 review, most of which was conducted as part of the USDA-funded National Jointed Goatgrass Research Program. Specific topics that were studied and reviewed here includeA. cylindricagenetics, especially as it relates to gene flow and hybridization rates with wheat and fertility of the resulting hybrids; vernalization requirements; seed dormancy, longevity, and germination requirements; competitiveness with wheat; and herbicide resistance acquired through evolution or gene flow from wheat. With respect to management, a wide variety of practices were evaluated, including various tillage types and frequencies; crop rotations, especially diversified wheat production systems that include spring-seeded annual crops; competitive wheat cultivars, seeding dates, seeding density, and row spacing; fertility management, including nitrogen application timing and placement; and field burning. Finally, many studies evaluated the use of herbicides, especially the introduction of imazamox in imidazolinone-resistant wheat cultivars, as well as comparison of adjuvant systems and application timings. In addition to the many management practices that were studied individually, several integrated management systems were evaluated that combined crop rotations, tillage, and herbicide programs. Between 1993 and 2013, weed scientists in 14 western states estimated that jointed goatgrass infestations decreased by 45% to 55% and attributed the reduction to the implementation of more diverse crop rotations, improved cultural practices, and use of imazamox-resistant wheat technology. This is evidence that the practical implications of the National Jointed Goatgrass Research Program have been successfully implemented by growers throughout the western United States.
Arf6-driven cell invasion is intrinsically linked to TRAK1-mediated mitochondrial anterograde trafficking to avoid oxidative catastrophe
Authors: Onodera Yasuhito , Nam Jin-Min , Horikawa Mei , Shirato Hiroki , Sabe Hisataka 
Published: July 2018

AbstractMitochondria dynamically alter their subcellular localization during cell movement, although the underlying mechanisms remain largely elusive. The small GTPase Arf6 and its signaling pathway involving AMAP1 promote cell invasion via integrin recycling. Here we show that the Arf6–AMAP1 pathway promote the anterograde trafficking of mitochondria. Blocking the Arf6-based pathway causes mitochondrial aggregation near the microtubule-organizing center, and subsequently induces detrimental reactive oxygen species (ROS) production, likely via a mitochondrial ROS-induced ROS release-like mechanism. The Arf6-based pathway promotes the localization of ILK to focal adhesions to block RhoT1–TRAK2 association, which controls mitochondrial retrograde trafficking. Blockade of the RhoT1–TRAK1 machinery, rather than RhoT1–TRAK2, impairs cell invasion, but not two-dimensional random cell migration. Weakly or non-invasive cells do not notably express TRAK proteins, whereas they clearly express their mRNAs. Our results identified a novel association between cell movement and mitochondrial dynamics, which is specific to invasion and is necessary for avoiding detrimental ROS production.
Deep-UV photoinduced chemical patterning at the micro- and nanoscale for directed self-assembly
Authors: Leuschel Benjamin , Gwiazda Agnieszka , Heni Wajdi , Diot Frédéric , Yu Shang-Yu , Bidaud Clémentine , Vonna Laurent , Ponche Arnaud , Haidara Hamidou , Soppera Olivier 
Published: July 2018

AbstractDeep-UV (DUV) laser patterning has been widely used in recent years for micro- and nanopatterning, taking advantage of the specific properties of irradiation with high-energy photons. In this paper, we show the usefulness of DUV laser patterning for preparing surfaces with controlled chemical properties at the micro- and nanoscale. Our motivation was to develop a simple and versatile method for chemical patterning at multiscales (from mm to nm) over relatively wide areas (mm2 to cm2). The chemical properties were provided by self-assembled monolayers (SAMs), prepared on glass or silicon wafers. We first investigated their modification under our irradiation conditions (ArF laser) using AFM, XPS and contact angle measurements. Photopatterning was then demonstrated with minimum feature sizes as small as 75 nm, and we showed the possibility to regraft a second SAM on the irradiated regions. Finally, we used these chemically patterned surfaces for directed self-assembly of several types of objects, such as block copolymers, sol-gel materials and liquids by vapor condensation.
A Data Mining-based Prognostic Algorithm for NAFLD-related Hepatoma Patients: A Nationwide Study by the Japan Study Group of NAFLD
Authors: Kawaguchi Takumi , Tokushige Katsutoshi , Hyogo Hideyuki , Aikata Hiroshi , Nakajima Tomoaki , Ono Masafumi , Kawanaka Miwa , Sawada Koji , Imajo Kento , Honda Koichi , Takahashi Hirokazu , Mori Kohjiroh , Tanaka Saiyu , Seko Yuya , Nozaki Yuichi , Kamada Yoshihiro , Fujii Hideki , Kawaguchi Atsushi , Takehara Tetsuo , Yanase Mikio , Sumida Yoshio , Eguchi Yuichiro , Seike Masataka , Yoneda Masato , Suzuki Yasuaki , Saibara Toshiji , Karino Yoshiyasu , Chayama Kazuaki , Hashimoto Etsuko , George Jacob , Torimura Takuji 
Published: July 2018

AbstractThe prognosis of patients with nonalcoholic fatty liver disease-related hepatocellular carcinoma (NAFLD-HCC) is intricately associated with various factors. We aimed to investigate the prognostic algorithm of NAFLD-HCC patients using a data-mining analysis. A total of 247 NAFLD-HCC patients diagnosed from 2000 to 2014 were registered from 17 medical institutions in Japan. Of these, 136 patients remained alive (Alive group) and 111 patients had died at the censor time point (Deceased group). The random forest analysis demonstrated that treatment for HCC and the serum albumin level were the first and second distinguishing factors between the Alive and Deceased groups. A decision-tree algorithm revealed that the best profile comprised treatment with hepatectomy or radiofrequency ablation and a serum albumin level ≥3.7 g/dL (Group 1). The second-best profile comprised treatment with hepatectomy or radiofrequency ablation and serum albumin levels <3.7 g/dL (Group 2). The 5-year overall survival rate was significantly higher in the Group 1 than in the Group 2. Thus, we demonstrated that curative treatment for HCC and serum albumin level >3.7 g/dL was the best prognostic profile for NAFLD-HCC patients. This novel prognostic algorithm for patients with NAFLD-HCC could be used for clinical management.
Myosin X is required for efficient melanoblast migration and melanoma initiation and metastasis
Authors: Tokuo Hiroshi , Bhawan Jag , Coluccio Lynne M. 
Published: July 2018

AbstractMyosin X (Myo10), an actin-associated molecular motor, has a clear role in filopodia induction and cell migrationin vitro, but its rolein vivoin mammals is not well understood. Here, we investigate the role of Myo10 in melanocyte lineage and melanoma induction. We found that Myo10 knockout (Myo10KO) mice exhibit a white spot on their belly caused by reduced melanoblast migration. Myo10KO mice crossed with available mice that conditionally express in melanocytes the BRAFV600Emutation combined with Pten silencing exhibited reduced melanoma development and metastasis, which extended medial survival time. Knockdown of Myo10 (Myo10kd) in B16F1 mouse melanoma cell lines decreased lung colonization after tail-vein injection. Myo10kd also inhibited long protrusion (LP) formation by reducing the transportation of its cargo molecule vasodilator-stimulated phosphoprotein (VASP) to the leading edge of migrating cells. These findings provide the first genetic evidence for the involvement of Myo10 not only in melanoblast migration, but also in melanoma development and metastasis.
Inhibition of angiogenesis in endothelial cells by Human Lysyl oxidase propeptide
Authors: Nareshkumar Ragavachetty Nagaraj , Sulochana Konerirajapuram Natarajan , Coral Karunakaran 
Published: July 2018

AbstractAngiogenesis is a critical process involved in normal physiology. Pathological angiogenesis is observed in vascular diseases and neoplasia. The propeptide domain of LOX (LOX-PP) has been shown to inhibit tumorigenesis in various cancers. In this study, we explored the role of both overexpressed and recombinant LOX-PP in naïve human umbilical vein endothelial cell with the addition of vascular endothelial growth factor (VEGF). Primarily, we observed a significant reduction in the angiogenesis signaling pathways upon LOX-PP overexpression by proteomic analysis. Further functional analysis showed that the VEGF induced cell proliferation, migration, adhesion and tube formation was inhibited by LOX-PP. Moreover, LOX-PP arrested cells at S-phase, reduced F-actin levels and decreased phosphorylation of focal adhesion kinase (FAK) and extracellular signal regulated kinase (ERK). The anti-angiogenic effect of LOX-PP was further confirmed by the reduction in the vascular network formation in chick chorioallantoic membrane (CAM). These results indicate that inhibition of angiogenesis events is not only achieved by overexpressing LOX-PP but also by addition of rLOX-PP. Taken together our findings discovered the anti-angiogenic role of LOX-PP in endothelial cells which suggests that harnessing this potential can be a promising strategy to inhibit angiogenesis.
Expression patterns and genetic variation of the ovine skeletal muscle transcriptome of sheep from five Spanish meat breeds
Authors: Noce A. , Cardoso T. F. , Manunza A. , Martínez A. , Cánovas A. , Pons A. , Bermejo L. A. , Landi V. , Sànchez A. , Jordana J. , Delgado J. V. , Adán S. , Capote J. , Vidal O. , Pazzola M. , Vacca G. M. , Casellas J. , Amills M. 
Published: July 2018

AbstractThe goal of the current study is to analyse the gene expression profile of the ovine skeletal muscle as well as to characterize the genetic variation of transcripts expressed in such tissue. This aim has been achieved by sequencing the longissimus dorsi transcriptomes of 50 sheep distributed in five pools representing the Canaria de Pelo, Roja Mallorquina, Gallega, Xisqueta and Ripollesa Spanish autochthonous breeds. Approximately, 363 million reads per pool have been produced and 71.9–82.9% have been successfully mapped to the ovine genome in a paired-end mode (2 × 75 bp). The 200 most expressed muscle transcripts (≈1% of the total transcript count) account for 51% (Canaria de Pelo) to 67% (Gallega) of the total ovine skeletal muscle mRNA expression. These highly expressed genes play key roles in pathways related with striated muscle contraction, gluconeogenesis, glycolysis, citric acid cycle and respiratory electron transport. RNA-Sequencing of muscle transcripts has also revealed that ~72% of the SNPs detected with this approach are shared by at least two pools, and 10% of them segregate in the five pools under analysis. Most of the substitutions detected by RNA-Seq are synonymous or missense and only a minority are predicted to have consequences on protein function.
The leaf transcriptome of fennel (Foeniculum vulgare Mill.) enables characterization of the t-anethole pathway and the discovery of microsatellites and single-nucleotide variants
Authors: Palumbo Fabio , Vannozzi Alessandro , Vitulo Nicola , Lucchin Margherita , Barcaccia Gianni 
Published: July 2018

AbstractFennel is a plant species of both agronomic and pharmaceutical interest that is characterized by a shortage of genetic and molecular data. Taking advantage of NGS technology, we sequenced and annotated the first fennel leaf transcriptome using material from four different lines and two different bioinformatic approaches: de novo and genome-guided transcriptome assembly. A reference transcriptome for assembly was produced by combining these two approaches. Among the 79,263 transcripts obtained, 47,775 were annotated using BLASTX analysis performed against the NR protein database subset with 11,853 transcripts representing putative full-length CDS. Bioinformatic analyses revealed 1,011 transcripts encoding transcription factors, mainly from the BHLH, MYB-related, C2H2, MYB, and ERF families, and 6,411 EST-SSR regions. Single-nucleotide variants of SNPs and indels were identified among the 8 samples at a frequency of 0.5 and 0.04 variants per Kb, respectively. Finally, the assembled transcripts were screened to identify genes related to the biosynthesis of t-anethole, a compound well-known for its nutraceutical and medical properties. For each of the 11 genes encoding structural enzymes in the t-anethole biosynthetic pathway, we identified at least one transcript showing a significant match. Overall, our work represents a treasure trove of information exploitable both for marker-assisted breeding and for in-depth studies on thousands of genes, including those involved in t-anethole biosynthesis.
Changing of the Genomic Pattern of Salmonella Enteritidis Strains Isolated in Brazil Over a 48 year-period revealed by Whole Genome SNP Analyses
Authors: Campioni Fábio , Cao Guojie , Kastanis George , Janies Daniel A. , Bergamini Alzira Maria Morato , Rodrigues Dália dos Prazeres , Stones Robert , Brown Eric , Allard Marc W. , Falcão Juliana Pfrimer 
Published: July 2018

AbstractSalmonella Enteritidis became the main serovar isolated from gastroenteritis cases in Brazil after the 90’s. In this study we used whole genome sequence analysis to determine the phylogenetic relationships among a collection of strains isolated in Brazil to identify possible genomic differences between the strains isolated in the pre and post-epidemic period. Also, we compared our data from strains isolated in Brazil to strains available in the public domain from other South American countries. Illumina technology was used to sequence the genome of 256 Salmonella Enteritidis strains isolated over a 48 year-period in Brazil, comprising the pre- and post-epidemic period. Phylogenetic analyses revealed distinct lineages for strains isolated before and after 1994. Moreover, the phage region SE20 that may be related to the emergence of Salmonella Enteritidis worldwide was present only in strains of the post-epidemic cluster. In conclusion, our results showed that the genomic profile of Salmonella Enteritidis strains isolated in Brazil shifted after 1994, replaced by a global epidemic group of strains. It may be hypothesized that the presence of the prophage SE20 might have conferred to these strains a better ability to colonize chicken and consequently to infect and cause disease in humans, which might better explain the increase in the number of S. Enteritidis cases in Brazil and other South American countries. However, to verify this hypothesis further studies are needed.
Characterization of low-mass companion HD 142527 B
Authors: Christiaens V. , Casassus S. , Absil O. , Kimeswenger S. , Gomez Gonzalez C. A. , Girard J. , Ramírez R. , Wertz O. , Zurlo A. , Wahhaj Z. , Flores C. , Salinas V. , Jordán A. , Mawet D. 
Published: July 2018

Context. The circumstellar disk of the Herbig Fe starHD 142527is host to several remarkable features including a warped inner disk, a 120 au-wide annular gap, a prominent dust trap and several spiral arms. A low-mass companion,HD 142527B, was also found orbiting the primary star at ~14 au.Aims. This study aims to better characterize this companion, which could help explain its impact on the peculiar geometry of the disk.Method. We observed the source with VLT/SINFONI inH + Kband in pupil-tracking mode. Data were post-processed with several algorithms based on angular differential imaging (ADI).Results.HD 142527B is conspicuously re-detected in most spectral channels, which enables us to extract the first medium-resolution spectrum of a low-mass companion within 0.″1 from its central star. Fitting our spectrum with both template and synthetic spectra suggests that the companion is a young M2.5 ± 1.0 star with an effective temperature of 3500 ± 100 K, possibly surrounded with a hot (1700 K) circum-secondary environment. Pre-main sequence evolutionary tracks provide a mass estimate of 0.34 ± 0.06M⊙, independent of the presence of a hot environment. However, the estimated stellar radius and age do depend on that assumption; we find a radius of 1.37 ± 0.05R⊙(resp. 1.96 ± 0.10R⊙) and an age of 1.8-0.5+1.2Myr (resp. 0.75 ± 0.25 Myr) in the case of the presence (resp. absence) of a hot environment contributing inH+K. Our new values for the mass and radius of the companion yield a mass accretion rate of 4.1–5.8 × 10−9M⊙yr−1(2–3% that of the primary).Conclusions. We have constrained the physical properties ofHD 142527B, thereby illustrating the potential for SINFONI+ADI to characterize faint close-in companions. The new spectral type makesHD 142527B a twin of the well-known TW Hya T Tauri star, and the revision of its mass to higher values further supports its role in shaping the disk.
Prostate Artery Embolization: Indication, Technique and Clinical Results
Authors: Teichgräber Ulf , Aschenbach René , Diamantis Ioannis , von Rundstedt Friedrich-Carl , Grimm Marc-Oliver , Franiel Tobias 
Published: July 2018

Background Prostate artery embolization (PAE) is a new embolization therapy to treat benign prostate syndrome (BPS).
Materials and Methods This review article presents the rationale and impact mechanism of PAE, criteria for patient selection, and discusses the anatomy of prostate arteries. The study results are seen in the context of complications and clinical partners.
Results Important preconditions for successful prostate artery embolization are a strict indication, precise knowledge of the anatomy of the pelvic arteries and advanced interventional-radiological skills. Several studies showed that urological parameters after prostate artery embolization improve at a similar level as for established post-surgical treatments. At the same time, it could be proven that prostate artery embolization has no impact on erectile function and is associated with a relatively low complication rate.
Conclusion PAE is increasingly developing to an alternative for the established surgical treatments in BPS patients.
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