bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Occurrence of Nosocomial Pathogens on Cell Phones of Healthcare Workers in an Iranian Tertiary Care Hospital
Authors: Khashei Reza , Ebrahim-Saraie Hadi Sedigh , Hadadi Mahtab , Ghayem Maysa , Shahraki Hadi Raeisi 
Published: September 2018

Background:Cell phones have become one of the necessary means of life and they are commonly used almost everywhere by every population. Colonized microorganisms on cell phones can be easily cross-transmitted. Given the widespread prevalence of nosocomial infections, this study aimed to determine the frequency of bacterial contamination and antibiotic resistance in cell phones of healthcare workers (HCWs) in a tertiary care hospital, from southwest of Iran.:In this cross-sectional study conducted between April and June 2016, sampling were performed from cell phones of 25 nurses and 75 medical students.Methods:Samples were collected from each cell phone by a moistened cotton swap dipped in normal saline prior and after decontamination with available alcohol-based handrubs. Identification of bacterial isolates was performed by conventional microbiologic methods. Antibiotic susceptibility pattern of the isolates was determined using the disk diffusion method.:The contamination rates of cell phones prior and after disinfection were 88% and 52%, respectively. Ninety-nine (71.2%) out of 139 isolated distinct bacterial colonies prior to cleaning were potentially nosocomial pathogens. Of them, staphylococci (88.9%) were the most prevalent bacteria, in which 40.9% were methicillin-resistant isolates. The majority of Gram-positive and - negative isolates were susceptible to the tested antimicrobials. Totally, contamination rate of cell phones was significantly reduced after decontamination. Regular disinfection of the hands and cell phones was significantly associated with reduction of colonization of the methicillin-resistant isolates.Result & Conclusion:These findings emphasize the restricted use of cell phones and encourage the higher compliance with hygienic practices in hospitals to reduce the risk of nosocomial infections.
A pesar de qué me decidí a estudiar una carrera del área química
Authors: Landeros Urbina Miguel Ángel 
Published: September 2018

<p>A pesar de que mi formación en las ciencias básicas no ha sido la más idónea y aunque en los niveles de educación media y básica pocos profesores se preocupan por inculcar en sus alumnos el interés...</p>
Optimasi produksi enzim ligninolitik dari medium limbah produksi Pleurotus ostreatus menggunakan metode respons permukaan (Optimization of ligninolytic enzyme production from Pleurotus ostreatus medium waste production using surface response methodology
Authors: PERWITASARI Urip , DIMAWARNITA Firda , RATNAKOMALA Shanti 
Published: September 2018

White rot fungi Pleurotus ostreatus has long been produced on a large scale for human consumption. This fungi is known to produce ligninolytic enzymes. The aim of this study was to utilize waste fungal medium from empty fruit bunch oil palm (EFBOP) for production of ligninolytic enzymes. Determination of the optimal conditions in this study used the design of statistical experiments Response Surface Method (RSM) with Design Expert® 10 software. Variables used in this research were EFBOP composition (0, 25, 50, 75, 100 %), part baglog (top, middle, bottom), and time of incubation (1, 2, and 3 month). The highest lignin peroxidase activity was 1.72 U/mL obtained on baglog composition with 50% EFBOP the top past of baglog after 2 months incubation. The highest manganese peroxidase activity was 23.00 U/mL obtained on baglog composition with 100% EFBOP at the bottom of baglog after 3 months incubation and the highest laccase activity was 0.14 U/mL on baglog composition with 100% EFBOP the top past of baglog after 1 month.[Keywords: Pleurotus ostreatus, ligninolytic enzyme, fungal medium waste, response surface methodology]. AbstrakJamur pelapuk putih Pleurotus ostreatus telah lama diproduksi skala besar untuk dikonsumsi. Jamur ini diketahui mampu menghasilkan enzim ligninolitik. Selama ini medium limbah produksi P. ostreatus belum dimanfaatkan. Penelitian ini bertujuan untuk memanfaatkan medium limbah produksi jamur tiram yang berbahan dasar tandan kosong kelapa sawit (TKKS) untuk produksi enzim ligninolitik. Penentuan kondisi optimal pada penelitian ini menggunakan desain eksperimen statistika Metode Respons Permukaan (Response Surface Method (RSM)) dengan software Design Expert® 10. Variabel yang digunakan dalam riset ini adalah konsentrasi TKKS (0, 25, 50, 75, 100 %), bagian baglog (atas, tengah, dan bawah), dan waktu inkubasi (1, 2, dan 3 bulan). Aktivitas lignin peroksidase tertinggi diperoleh pada medium dengan komposisi 50% medium pada bagian atas baglog setelah 2 bulan inkubasi dengan aktivitas sebesar 1,72 U/mL. Aktivitas mangan peroksidase tertinggi diperoleh pada medium komposisi 100% TKKS pada bagian bawah baglog setelah 3 bulan inkubasi sebesar 23,00 U/mL, dan lakase tertinggi pada medium komposisi 100% TKKS pada bagian atas baglog setelah 1 bulan inkubasi, yaitu sebesar 0,14 U/mL.[Kata kunci: Pleurotus ostreatus, enzim ligninolitik, limbah media jamur, Metode Respons Permukaan]
Identifikasi isolat Phytophthora asal kakao Identification of isolates of Phytophthora from cocoa
Authors: UMAYAH Abu , PURWANTARA Agus 
Published: September 2018

Summary Phytophthora spp. are responsible for some serious diseases of cocoa including pod rot, stem canker, leaf blight, seedling blight, and chupon wilt. Eight species of Phytophthora have been isolated from diseased cocoa worldwide, even though only three species cause most losses in cocoa production. Twenty isolates of Phytophthora sp. were isolated from various parts of the cocoa tree collected from six cocoa producing provinces in Indonesia, viz. North Sumatera, Lampung, West Java, East Java, South Sulawesi and Southeast Sulawesi. All isolates were then identified using their morphological charac-teristics and it was concluded that all of the isolates are Phytophthora palmivora. This identification was then confirmed with molecular identification by amplification of ITS of rDNA of the isolates with primers ITS 4 and ITS 5, followed by restriction of the amplicon with enzymes. The molecular identification confirmed that all isolates are P. palmivora. Ringkasan Phytophthora spp. merupakan penyebab beberapa penyakit penting pada kakao, termasuk busuk buah, kanker batang, hawar daun, hawar bibit, dan layu tunas air. Delapan spesies Phytophthora telah berhasil diisolasi dari tanaman kakao sakit di seluruh dunia, meskipun hanya tiga spesies yang meng-akibatkan kehilangan produksi kakao yang nyata. Dua puluh isolat Phytophthora sp. telah diisolasi dari berbagai bagian tanaman kakao yang dikumpulkan dari enam provinsi sentra produksi kakao di Indonesia, yaitu Sumatera Utara, Lampung, Jawa Barat, Jawa Timur, Sulawesi Selatan dan Sulawesi Tenggara. Semua isolat diidentifikasi berdasarkan sifat-sifat morfologi dan dapat disimpulkan bahwa semua isolat adalah Phytophthora palmivora. Identifikasi selanjutnya dilakukan secara molekuler dengan amplifikasi daerah ITS dari rDNA isolat menggunakan pasangan primer ITS 4 dan ITS 5, kemudian diikuti dengan pemotongan amplikon menggunakan enzim restriksi. Identifikasi molekuler juga menun-jukkan bahwa semua isolat Phytophthora penyebab penyakit pada kakao adalah P. palmivora.
Molecular markers and their application for DNA fingerprinting and genetic diversity studies in Coffea species Marka molekuler dan penerapannya untuk studi sidik jari DNA dan keragaman genetik pada spesies Coffea
Authors: PRIYONO . , PUTRANTO Riza Arief 
Published: September 2018

AbstrakStrategi klasik yang meliputi perbandingan anatomi, fisiologi dan sitogenetika telah banyak diterapkan untuk mengidentifikasi karakter tertentu serta untuk menentukan keragaman dan hubungan antar dan intra spesies. Namun, saat ini penanda molekuler telah melengkapi strategi sebelumnya dengan sangat cepat. Berbagai jenis penanda molekuler digunakan untuk menilai tingkat polimorfisme DNA. Penanda molekuler ini diklasifikasikan sebagai penanda berbasis hibridisasi dan berbasis Polymerase Chain Reaction (PCR). Dalam beberapa tahun terakhir, sistem penanda DNA yang berbeda seperti Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) yang juga disebut Mikrosatelit, Single Nucleotide Polymorphims (SNPs) dan lain-lain telah dikembangkan dan diterapkan pada berbagai spesies tanaman. Penanda molekuler ini dapat digunakan untuk sidik jari DNA dan studi keragaman genetik. Sidik jari berdasarkan DNA telah banyak digunakan dalam ilmu forensik, juga memiliki berbagai aplikasi dalam pemuliaan tanaman. Tulisan ini memberikan overview tentang berbagai penanda molekuler dan aplikasinya untuk sidik jari dan kajian keragaman genetik tanaman berdasarkan DNA pada berbagai spesies tanaman, dan secara khusus pada Coffea sp.AbstractConventional strategies including comparative anatomy, physiology and cytogenetics were applied to identify the certain character as well as to determine inter- and intra-species diversity and relationships. However, more recently molecular markers have very rapidly complemented the previous strategies. Various types of molecular markers are used to assess DNA polymorphism. They are classified as hybridization-based markers and polymerase chain reaction (PCR) based markers. In recent years, different DNA marker systems such as Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) which also called as microsatellites, Single Nucleotide Polymorphims (SNPs) and others have been developed and applied to a range of plant species. These molecular markers can be used for DNA fingerprinting and genetic diversity study. DNA fingerprinting has been widely used in forensic science, but is has also a variety of application in plant breeding. This paper provides an overview about various molecular markers and their application for DNA plant fingerprinting and genetic diversity, especially in Coffea sp.
Identifikasi dan pencegahan kontaminasi pada kultur cair sistem perendaman sesaat Identification and prevention of contamination in liquid culture of temporary immersion system
Authors: SINTA Masna Maya , RIYADI Imron , SUMARYONO . 
Published: September 2018

AbstractLiquid culture is commonly used to scale up in vitro culture production as well as to optimize the developmental phase of plant in vitro culture. One of the liquid cultures that has been used widely is temporary immersion system (TIS). The main problem of liquid culture is contamination. The use of antibiotics sometimes controls the contaminants less effectively and hinders the growth of plant culture. The purpose of this research was to determine sources of contaminant on whole sequence of TIS to identify and to prevent the emergence of the contaminants. Sampling method was applied to each section and stage of TIS culture and the contaminants found were identified. The results revealed that compartment of TIS was the main source of contaminant (100%). Furthermore, from all components of TIS compartment, washer (a small ring seal connecting screen disc and basket) was the main source of TIS contaminant (41.2%). Four contaminants found were identified as Bacillus macerans, Bacillus megaterium, Bacillus sphaericus and Bacillus firmus. Two times sterilization of washer in an autoclave at temperature of 121 oC and air pressure of 1 kg/cm2 for 20 minutes before and after being installed reduced the contamination level on TIS culture significantly.AbstrakKultur cair umumnya digunakan untuk meningkatkan skala produksi dan mengoptimalkan fase perkembangan kultur in vitro tanaman. Salah satu jenis kultur cair yang banyak digunakan adalah sistem perendaman sesaat (SPS). Masalah utama dalam kultur cair adalah kontaminasi. Penggunaan antibiotika terkadang kurang efektif dalam me-ngendalikan kontaminan dan menghambat pertumbuhan kultur tanaman. Tujuan dari penelitian ini adalah untuk mengetahui sumber kontaminan pada seluruh rangkaian kultur SPS serta mengidentifikasi dan mencegah munculnya kontaminan tersebut. Metode yang digunakan adalah pengambilan contoh pada tiap bagian dan fase kultur SPS, serta kontaminan yang ditemukan kemudian diidentifikasi. Hasil penelitian memperlihatkan bahwa kompartemen SPS merupakan sumber utama kontaminan (100%). Selanjutnya, dari seluruh komponen kompartemen SPS, washer (cincin penutup yang menghubungkan penyaring dan keranjang) di dalam rangkaian SPS merupakan sumber utama kontaminan (41,2%). Empat kontaminan yang ditemukan diidentifikasi sebagai Bacillus macerans, Bacillus megaterium, Bacillus sphaericus dan Bacillus firmus. Sterilisasi cincin penutup sebanyak dua kali dalam autoklaf pada suhu 121 oC dan tekanan udara 1 kg/cm2selama 20 menit sebelum dan sesudah dirangkai secara nyata menurunkan tingkat konta-minasi pada kultur SPS.
Genetic mapping studies in Coffea sp using molecular marker methods Studi peta genetik pada Coffea sp. menggunakan metode penanda molekuler
Authors: PRIYONO . , PUTRANTO Riza Arief 
Published: September 2018

AbstrakAnalisis genetik telah menjadi alat yang penting dalam pemuliaan tanaman untuk perbaikan sifat penting tanaman. Salah satu potensi terbesar dari analisis tersebut adalah identifikasi penanda molekuler yang berguna untuk pemetaan genetik. Pemetaan genetik merupakan salah satu langkah penting dari analisis genetik. Intisari dari semua pemetaan genetik adalah menempatkan koleksi pe- nanda molekuler pada posisi tertentu dalam genom. Hal tersebut dapat kemudian digunakan untuk meng- identifikasi lokus sifat kuantitatif (QTLs) dengan memanfaatan keragaman genetik alami yang tersedia dan meningkatkan sifat-sifat penting serta berharga. Sampai saat ini, tiga belas peta genetik telah dipublikasi dan tersedia pada Coffea sp. yang menciptakan database besar untuk kerangka genetik. Sebuah peta genetik terbaru dengan akses terbuka dan berfungsi sebagai referensi telah dibangun oleh International Coffee Genomics Network (ICGN). Peta tersebut tediri dari 3230 lokus, dengan panjang peta 1471 cM (1cm ~ 500 Kb) serta kepadatan satu penanda setiap 220 Kb. Peta-peta genetik pada tanaman kopi telah digunakan dari karakterisasi gen hingga analisis komparatif genom dengan spesies tanaman yang berbeda. Saat ini, pesatnya kemajuan teknologi New Genome Sequencing (NGS) untuk sekuensing DNA dan RNA memungkinkan validasi dari peta-peta genetik untuk prediksi QTLs serta gen-gen yang membawa sifat penting Coffea sp.AbstractGenetic analysis has become an important tool in plant breeding for crop improvement. One of their greatest potential appears to be the identification of molecular markers useful for genetic mapping. Genetic mapping is one of important steps in genetic analysis. The essence of all genetic mapping is to place a collection of molecular markers onto their respective positions on the genome. Thus, it leads to identification of new quantitative trait loci (QTLs) by making benefits of natural available genetic diversity.and to improve important and valuable traits. Until present, thirteen genetic maps were published and available in Coffea sp. creating a huge database for genetic framework. One most recent and open reference genetic map for robusta coffee has been generated by the International Coffee Genomics Network (ICGN) comprising 3230 loci, genetic size 1471 cM (1cM ~500 Kb), with an average density close to one marker every 220 Kb. The Coffea genetic maps have been utilized from gene characterization to genomic comparative analysis with different plant species. Nowadays, the feasibility of NGS for DNA and RNA sequencing allow the validation of genetic map related to the prediction of QTLs and adjacent genes related to important traits for Coffea sp.
Non-Alcoholic Fatty Liver Disease, Bile Acids and Intestinal Microbiota
Authors: Maslennikov R. V. , Evsyutina Yu. V. 
Published: September 2018

Aim.  The aim of the review is to present current data on the relationship between non-alcoholic fatty liver disease (NAFLD) with the metabolic disorders of bile acids (BA) and changes in the composition of the intestinal microbiota.Background.  NAFLD is accompanied by a change in the intestinal microbiotic composition: the proportion of taxa deconjugating BAs increases, while the proportion of taxa converting primary BAs to secondary ones decreases. The number of bacteria forming lipopolysaccharide (LPS) also increases. LPS, entering the liver with the portal vein blood, promotes the development of its inflammation and insulin resistance. The disturbance of bile acid metabolism through the effect on the FXR and TGR5 receptors also leads to insulin resistance and liver steatosis. FXR probiotics and agonists are promising drugs for the NAFLD treatment.Conclusion.  In the course of NAFLD, a change in the composition of the intestinal microbiota is observed, which contributes to the development of inflammation in the liver and disrupts the metabolism of bile acids, leading to insulin resistance.
Late Saalian and Eemian Interglacial at the Struga site (Garwolin Plain, central Poland)
Authors: Bober Aleksandra , Pidek Irena Agnieszka , Żarski Marcin 
Published: September 2018

AbstractThe paper reports pollen analyses of 47 samples from palaeolake sediments at WH-15 Struga near Puznówka on the Garwolin Plain in central Poland. The pollen succession covers Late Saalian (MIS-6) and fully developed Eemian (MIS-5e) successions. The Late Saalian section is well developed (more than 2 m thick) and contains sub-zones reflecting the alternating dominance of steppe-tundra and boreal forest communities. The analysed Eemian succession is an uncommon succession described as a variant with early appearance and culmination of Tilia. In these terms, the successions of WH-15 Struga resemble those of sites of the Eemian interglacial known from the vicinity of Warsaw: Błonie, Warszawa-Żoliborz and Warszawa-Wola. The WH-15 Struga site is one of several recently discovered fossil sites of Eemian lakes on the Garwolin Plain, constituting the southern fragment of the extensive Eemian lakeland in the Polish Lowland.
Bird communities and vegetation composition in natural and semi-natural forests of megalopolis: correlations and comparisons of diversity indices (Kyiv city, Ukraine)
Authors: Blinkova Olena , Shupova Tetyana 
Published: September 2018

Abstract
Assessment of correlations and comparisons of diversity indices between birds communities and vegetation composition of the forest ecosystem is an important subject of synecological research to identify the intensity of human impact on the flora and fauna in megalopolis. Urban recreation is one of the major causes of violation of the structural and functional integrity of the forest ecosystem. Studies on avian complex and phytocoenosis have focused on the impact of urban recreation on the soil surface, compositions of trees, shrub and herbaceous layers and species, trophic and ecological compositions of nesting-birds and feeding-birds communities. This article compares the measurement of the diversity of bird communities and forestry vegetation (diversity indices, dominance indices, evenness indices) in the natural and semi-natural forests of the Kyiv city on a gradient of recreational transformation. The stands formed consist of Quercus robur L., Carpinus betulus L., Acer platanoides L., Tilia cordata L., Ulmus glabra Huds., Fraxinus excelsior L. and Prunus avium L. The floristic list comprised 65 grass species. A total of 49 species of avifauna were found, including 44 species that were protected by the Bern, Bonn and Washington Conventions. There were significant correlations between vertical and horizontal heterogeneities of tree distribution and abundance, species richness and nesting density of birds. The interrelationship between species diversity of birds and floristic richness was also confirmed.
Underlying causes and the impacts of disaster events (floods) on fertility decision in rural Bangladesh
Authors: Haq Shah Md. Atiqul 
Published: September 2018

Abstract
The study attempts to uncover how people living in vulnerable areas address the relationship between the impacts of extreme weather events (floods) and fertility preference. The study selected a village, Sharat Pur from Sunamganj District, which is highly vulnerable to the adverse impacts of flooding. The study gathered information from 158 respondents by using a semi-structured questionnaire and in-depth interviews. With the small sample size the study used descriptive statistics and qualitative analysis. Findings show that there is still a preference for more sons to recover the damage caused from the impacts of floods. However, people think having a large family is a burden. They emphasize controlling family size through family planning programs and do not take into account the risk of children dying affecting their desire to have additional children. Therefore they consider having more children especially sons as a gift from God and the occurrence of flood events as the wish of God. The intention of the study is not to generalize findings but to comprehend the underlying mechanism between disaster events and fertility behavior. The study collected information from a very small sample size. Future studies might consider a large sample size and explore more deeply the mechanism between the different disaster events and fertility decisions by using a comparative analysis between regions vulnerable to different extreme weather events and not vulnerable to extreme weather events within Bangladesh, and comparing Bangladesh with other South Asian countries.
From "Withered Wood" to "Dead Ashes": Burning Bodies, Metamorphosis, and the Ritual Production of Power
Authors: Meulenbeld Mark r.e. 
Published: September 2018

Cet article porte sur le rituel taoïste de transformation du corps tel qu'il est couramment accompli dans la province du Hunan, et aussi tel qu'il apparaît dans une série de sources canoniques et apocryphes. L'auteur soutient que la métaphore rituelle constitue une clé pour comprendre les « formes poétiques du pouvoir » du taoïsme. En considérant la transformation du corps en tant que narration discursivement structurée d'une incinération sacrificielle, l'auteur montre combien le langage de la victime humaine brûlée participe de l'efficacité rituelle, en particulier dans le cas du rituel pour faire venir la pluie. Les narrations qui, historiquement, précédent les accomplissements modernes du rituel de la métamorphose, peuvent être mieux comprises si on les situe dans un contexte social tel que celui du Hunan. Autrement dit, à première vue, la narration dans les textes classiques semble construire la transformation du corps en tant que discours autonome, exclusivement tourné vers la transformation intérieure de personnages reclus. Mais compte tenu du fait que ce genre de discours sur la transformation intérieure fait l'objet de célébrations publiques se déroulant dans un cadre social de patronage du rituel, et étant donné l'efficacité rituelle attendue de telles performances de nos jours, il est peut-être plus pertinent de considérer les textes classiques comme les produits d'un environnement où les pratiques spirituelles autorisaient les pratiquants à pouvoir incarner les forces commandant sur les puissances cosmiques — puissances qui pouvaient être utilisées pour le bénéfice de la société.