bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the GENE-UP®Cronobacter Method for the Detection of Cronobacter Species in Select Foods and Environmental Surfaces: Collaborative Study, First Action 2019.01
Authors: Johnson Ronald , Mills John , Pittet Jean-Louis , Rannou Maryse , Bird Patrick , Nelson Maria 
Published: August 2019

Abstract
Background: The GENE-UP®Cronobacter assay (Performance Tested MethodSM 081801) is a real-time PCR technology for the rapid detection of Cronobacter species in select foods and environmental surfaces. Objective: The purpose of this validation was to evaluate the method’s interlaboratory performance and submit the result to AOAC INTERNATIONAL for adoption as a First Action Official MethodSM for the detection of Cronobacter species in select foods and environmental surfaces. Method: The GENE-UP method was evaluated in a multilaboratory study as part of the AFNOR NF VALIDATION certification process (NF102) following ISO 16140-2:2016 using unpaired test portions for one food matrix, reconstituted infant formula containing probiotics. The candidate method was compared to the ISO 22964:2017 reference method. Sixteen participants from fifteen laboratories throughout the European Union participated. Three levels of contamination were evaluated: a noninoculated control level (0 CFU/target test portion), a low contamination level (approximately 2 CFU/target test portion), and a high contamination level (approximately 10 CFU/target test portion). Data from that study were analyzed according to the probability of detection (POD) statistical model as presented in the AOAC validation guidelines. The difference in laboratory POD (dLPODC) values with 95% confidence intervals across collaborators was calculated for each level between the candidate and reference method results and between the candidate presumptive and confirmed results. Results: The dLPODC values with 95% confidence intervals were 0.00 (–0.03, 0.03), –0.08 (–0.19, 0.02), and 0.00 (–0.03, 0.03) for the noninoculated, low, and high contamination levels, respectively. Conclusions: The dLPODC results indicate no significant difference between the candidate method and the reference method or between presumptive and confirmed results for all three levels of contamination. Highlights: The GENE-UP Cronobacter assay provides industry with a rapid, easy to use method for the rapid detection of Cronobacter in a wide range of products and environmental samples.
Simultaneous Determination of 12 Bioactive Constituents in Bupi Yiqi No. 1 Recipe by Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry
Authors: Xie Qingling , Li Bin , Huang Feibing , Cao Mengru , Yuan Hanwen , Liu Yongbei , Li Wei , Qiu Yixing , Zhou Xiaoling , Man Rongyong , Wang Wei , Zhang Weining 
Published: August 2019

Abstract
Background: Bupi Yiqi No. 1 Recipe (BPYQ), a traditional Chinese medicine formula, has been widely used as a treatment of multidrug resistance during chemotherapy in colorectal cancer and for the prevention of gastrointestinal cancer recurrence for decades. Objective: In this study, a rapid, reliable, and accurate ultrahigh-performance LC (UHPLC) coupled with electrospray ionization (ESI)–tandem MS (MS/MS) method was developed for the simultaneous determination of 12 major bioactive components, including protocatechuic acid, astilbin, rutin, calycosin-7-O-β-D-glucoside, ginsenoside Re, ononin, isoliquiritigenin, calycosin, apigenin, ginsenoside Rb1, formononetin, and glycyrrhizic acid in BPYQ. Methods: The chromatographic separation of the analytes was achieved on a Poroshell 120 SB-Aq column (50 × 2.1 mm, 1.7 µm) with a mobile phase of acetonitrile and 0.1% (v/v) formic acid aqueous solution. The flow rate and column temperature were set at 0.4 mL/min and 30°C, respectively. Mass spectrometric detection of the analyses was performed on multiple reaction monitoring mode in positive and negative ESI mode. Results: The established UHPLC–ESI–MS/MS method was validated in terms of the linearity, precision, repeatability, stability, and accuracy. All calibration curves of the 12 compounds showed good linearity, with correlation coefficients (r) greater than 0.9980 within the test ranges. The LODs and LOQs for the 12 compounds were in the ranges of 0.08–1.32 and 0.27–5.28 ng/mL, respectively. The average recoveries of all the standard compounds were between 98.4 and 102.9%, and their relative SD values ranged from 1.24 to 3.78%. Conclusions: The proposed method can provide a meaningful basis for the QC of BPYQ. Highlights: The established UHPLC-ESI-MS/MS method was demonstrated to be a powerful tool for quantifying the 12 compounds in BPYQ.
Qu’est-ce que la nature qu’on cherche à conserver? Une approche sémiologique de l’action écologique
Authors: Ducarme Frédéric 
Published: August 2019

L’écologie est passée au cours du XXe siècle de science descriptive à science de l’action. Cette métamorphose contraint à adapter tout un vocabulaire théorique à l’épreuve du concret. Or, cette translation n’est pas facile. Si la large majorité des acteurs sociaux s’accordent quant à la nécessité de « protéger la nature », des controverses profondes s’élèvent dès qu’il s’agit de mettre en action cette préservation : que doit-on faire, sur quoi doit-on agir quand on veut protéger « la nature »? Qu’est-ce que la « nature », en somme? C’est à cette question que ce texte propose de répondre, en retraçant tout d’abord la généalogie de ce terme dont le sens n’a jamais été clair, puis en isolant un certain nombre de définitions dominantes, renvoyant chacune à une conception de la nature bien spécifique, et supposant donc des actions de protection distinctes. Plutôt que d’arbitrer de manière jupitérienne (comme s’y sont essayés sans succès de nombreux penseurs), nous proposons d’intégrer toute cette complexité du phénomène nature dans les démarches de protection de la nature, qui doit être pensée dans une acception large, transdisciplinaire et transculturelle.
Prevalence of near-death experiences in people with and without REM sleep intrusion
Authors: Kondziella Daniel , Dreier Jens P. , Olsen Markus Harboe 
Published: August 2019

Background
The origin and prevalence of near-death experiences are unknown. A recent study suggested a link with REM sleep intrusion but was criticized for its selection of control participants. We therefore assessed the association of REM intrusion and near-death experiences with different methods.

Methods
Using a crowd-sourcing platform, we recruited 1,034 lay people from 35 countries to investigate the prevalence of near-death experiences and self-reported REM sleep intrusion. Reports were validated using the Greyson Near-Death Experiences Scale (GNDES) with ≥7 points as cut-off for near-death experiences.

Results
Near-death experiences were reported by 106 of 1,034 participants (10%; 95% CI [8.5–12%]). Evidence of REM intrusion was more common in people with near-death experiences (n = 50∕106; 47%) than in people with experiences with 6 points or less on the GNDES (n = 47∕183; 26%) or in those without such experiences (n = 107∕744; 14%; p = < 0.0001). Following multivariate regression analysis to adjust for age, gender, place of residence, employment and perceived danger, this association remained highly significant; people with REM intrusion were more likely to exhibit near-death experiences than those without (OR 2.85; 95% CI [1.68–4.88]; p = 0.0001).

Discussion
Using a crowd-sourcing approach, we found a prevalence of near-death experiences of 10%. While age, gender, place of residence, employment status and perceived threat do not seem to influence the prevalence of near-death experiences, we confirmed a possible association with REM sleep intrusion.
Characterization of Polyphenols and Mineral Contents in Three Medicinal Weeds
Authors: Sahu Yaman Kumar , Patel Khageshwar Singh , Martín-Ramos Pablo , Towett Erick K. 
Published: August 2019

Aims: Common weeds Rorippa palustris (L.) Besser, Euphorbia rothiana Spreng. and Schoenoplectiella articulata (L.) Lye are used for food, medicinal, green biofertilizer and biosorbent applications. In this work, their polyphenol and mineral contents have been characterized.
Methodology: Samples from aforementioned three plants were manually collected in Raipur city (CG, India) and processed for the analyses. Folin-Ciocalteu and aluminum chloride were used for the spectrophotometric determination of polyphenols. The mineral contents were quantified by X-ray fluorescence.
Results: The total concentration of 20 elements (viz. P, S, Cl, As, Se, K, Rb, Mg, Ca, Sr, Ba, Al, Ti, Cr, Mn, Fe, Co, Zn, Mo and Pb), total polyphenol and flavonoid contents in the leaves ranged from 46372 to 71501, from 47877 to 73791 and from 1950 to 9400 mg/kg, respectively. Remarkable concentrations of several nutrients (P, S, Cl, K, Mg, Ca and Fe) were observed.
Conclusion: The biomass from medicinal weeds R. palustris, E. rothiana and S. articulata featured very high K, Ca and Fe contents. Other nutrients (polyphenols, flavonoids, P, S, Cl and Mg) were identified at moderate levels. These species may hold promise as bioindicators.
Neural networks associated with body composition in frontotemporal dementia
Authors: Ahmed Rebekah M. , Landin‐Romero Ramon , Liang Cheng T. , Keogh Julia M. , Henning Elana , Strikwerda‐Brown Cherie , Devenney Emma M. , Hodges John R. , Kiernan Matthew C. , Farooqi I. Sadaf , Piguet Olivier 
Published: August 2019

AbstractBackgroundFrontotemporal dementia (FTD) is associated with complex changes in eating behavior and metabolism, which potentially affect disease pathogenesis and survival. It is currently not known if body composition changes and changes in fat deposition also exist in FTD, the relationship of these changes in eating behavior and appetite, and whether these changes are centrally mediated.MethodsBody composition was measured in 28 people with behavioral‐variant frontotemporal dementia (bvFTD), 16 with Alzheimer’s disease (AD), and 19 healthy controls, using dual energy x‐ray absorptiometry. Changes in body composition were correlated to brain grey matter atrophy using voxel‐based morphometry on high‐resolution magnetic resonance imaging.ResultsBehavioral‐variant FTD was characterized by changes in body composition, with increased total fat mass, visceral adipose tissue area (VAT area), and android: gynoid ratio compared to control and AD participants (all P values < 0.05). Changes in body composition correlated to abnormal eating behavior and behavioral change (P < 0.01) and functional decline (P < 0.01). Changes in body composition also correlated to grey matter atrophy involving a distributed neural network that included the hippocampus, amygdala, nucleus accumbens, insula, cingulate, and cerebellum – structures known to be central to autonomic control – as well as the thalamus, putamen, accumbens, and caudate, which are involved in reward processing.ConclusionsChanges in body composition and fat deposition extend the clinical phenomenology in bvFTD beyond cognition and behavior, with changes associated with changes in reward and autonomic processing suggesting that these deficits may be central in FTD
Exosome‐Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing
Authors: Kim Hyosuk , Wang Sun Young , Kwak Gijung , Yang Yoosoo , Kwon Ick Chan , Kim Sun Hwa 
Published: August 2019

AbstractMacrophages (Mϕs) critically contribute to wound healing by coordinating inflammatory, proliferative, and angiogenic processes. A proper switch from proinflammatory M1 to anti‐inflammatory M2 dominant Mϕs accelerates the wound healing processes leading to favorable wound‐care outcomes. Herein, an exosome‐guided cell reprogramming technique is proposed to directly convert M1 to M2 Mϕs for effective wound management. The M2 Mϕ‐derived exosomes (M2‐Exo) induce a complete conversion of M1 to M2 Mϕs in vitro. The reprogrammed M2 Mϕs turn Arginase (M2‐marker) and iNOS (M1‐marker) on and off, respectively, and exhibit distinct phenotypic and functional features of M2 Mϕs. M2‐Exo has not only Mϕ reprogramming factors but also various cytokines and growth factors promoting wound repair. After subcutaneous administration of M2‐Exo into the wound edge, the local populations of M1 and M2 Mϕs are markedly decreased and increased, respectively, showing a successful exosome‐guided switch to M2 Mϕ polarization. The direct conversion of M1 to M2 Mϕs at the wound site accelerates wound healing by enhancing angiogenesis, re‐epithelialization, and collagen deposition. The Mϕ phenotype switching induced by exosomes possessing the excellent cell reprogramming capability and innate biocompatibility can be a promising therapeutic approach for various inflammation‐associated disorders by regulating the balance between pro‐ versus anti‐inflammatory Mϕs.
A Universal Strategy for Improving the Energy Transmission Efficiency and Load Power of Triboelectric Nanogenerators
Authors: Gao Fangfang , Zhang Zheng , Liao Qingliang , Zhang Guangjie , Kang Zhuo , Zhao Xuan , Xun Xiaochen , Zhao Zening , Xu Liangxu , Xue Lingfang , Zhang Yue 
Published: August 2019

AbstractTriboelectric nanogenerators (TENGs) have recently been invented as a potential energy technology for harvesting low‐frequency mechanical energy. The load power acquired from a TENG is far less than the maximum output power of the TENG for the large internal impedance and impedance mismatch, and this difference results in an extremely low energy transmission efficiency. Here, a universal strategy is proposed for improving the energy transmission efficiency and load power through dielectric material design, including a reduction in the effective thickness and the directional alignment of the electric dipole. This strategy reduces the internal impedances of TENGs with different modes and results in the improvement of energy transmission efficiency and load power. According to this strategy, the internal impedance of an as‐fabricated TENG is reduced from 16 to 1.3 MΩ, and the energy transmission efficiency is enhanced from 22.46% to 99.5%. Moreover, the load power under 1 MΩ resistance is improved from 0.014 to 0.251 µW, an increase of 18 times. The strategy not only opens a universal and new road to power management, but also paves the way for the industrial applications of TENGs.
Boosting the Activity of BaCo0.4Fe0.4Zr0.1Y0.1O3−δ Perovskite for Oxygen Reduction Reactions at Low‐to‐Intermediate Temperatures through Tuning B‐Site Cation Deficiency
Authors: Kuai Xu , Yang Guangming , Chen Yubo , Sun Hainan , Dai Jie , Song Yufei , Ran Ran , Wang Wei , Zhou Wei , Shao Zongping 
Published: August 2019

AbstractDoped perovskite oxides with the general formula of AxA′1−xByB′1−yO3 have been extensively exploited as the cathode materials of solid oxide fuel cells (SOFCs), but the performance at low‐to‐medium temperatures still needs improvement. BaCo0.4Fe0.4Zr0.1Y0.1O3−δ (BCFZY) has been recently reported to show promising oxygen reduction reaction (ORR) activity under SOFCs' operating conditions. Here, it is reported that the activity of BCFZY can be further boosted via introducing a slight B‐site cation deficiency into the oxide lattice, and such an improvement is assigned to an increase in oxygen mobility that brings enhancement in both surface exchange and bulk diffusion kinetics. Specifically, materials with the nominal composition of Ba(Co0.4Fe0.4Zr0.1Y0.1)0.975O3−δ and Ba(Co0.4Fe0.4Zr0.1Y0.1)0.95O3−δ show significantly improved activity for ORR at reduced temperatures with the area specific resistances of 0.011 and 0.024 Ω cm2 at 600 °C, as a comparison of 0.042 Ω cm2 for the cation stoichiometric BCFZY. Excessive B‐site deficiencies, however, lead to the formation of impurity phases, which cause a block for charge transfer and, consequently, a reduction in electrode performance. Introducing a B‐site cation deficiency is a promising way to optimize the activity of perovskite oxides for ORR at reduced temperatures, but the degree of deficiency shall be carefully tuned.
Absorption and regeneration studies of chemical solvents based on dimethylethanolamine and diethylethanolamine for carbon dioxide capture
Authors: Baltar Alberto , Gómez‐Díaz Diego , Navaza José M. , Rumbo Antonio 
Published: August 2019

AbstractPresent research work analyses a new chemical solvents based on the use of two tertiary amines (dimethylethanolamine and diethylethanolamine) for carbon dioxide (CO2) capture by chemical absorption taking into account the absorption behavior and the solvent regeneration degree. Regarding the first study, the absorption rate is related with the reaction mechanism at different CO2 loading. Other type of influences such as the type and amine concentration, and gas flow rate fed to the contactor were studied. In relation with solvent regeneration, several experimental procedures have been employed in order to avoid the negative characteristics of loaded‐solvent stripping. The results show the important weight of amines acidity constant upon the regeneration yield.
Dynamic Control of Chiral Space Through Local Symmetry Breaking in a Rotaxane Organocatalyst
Authors: Dommaschk Marcel , Echavarren Javier , Leigh David A. , Marcos Vanesa , Singleton Thomas A. 
Published: August 2019

AbstractWe report on a switchable rotaxane molecular shuttle that features a pseudo‐meso 2,5‐disubstituted pyrrolidine catalytic unit on the axle whose local symmetry is broken according to the position of a threaded benzylic amide macrocycle. The macrocycle can be selectively switched (with light in one direction; with catalytic acid in the other) with high fidelity between binding sites located to either side of the pyrrolidine unit. The position of the macrocycle dictates the facial bias of the rotaxane‐catalyzed conjugate addition of aldehydes to vinyl sulfones. The pseudo‐meso non‐interlocked thread does not afford significant selectivity as a catalyst (2–14 % ee), whereas the rotaxane affords selectivities of up to 40 % ee with switching of the position of the macrocycle changing the handedness of the product formed (up to 60 % Δee).
Colloidal Synthesis and Optical Properties of All‐Inorganic Low‐Dimensional Cesium Copper Halide Nanocrystals
Authors: Cheng Pengfei , Sun Lei , Feng Lu , Yang Songqiu , Yang Yang , Zheng Daoyuan , Zhao Yang , Sang Youbao , Zhang Ruiling , Wei Donghui , Deng Weiqiao , Han Keli 
Published: August 2019

AbstractLow‐dimensional metal halides have recently attracted extensive attention owing to their unique structure and photoelectric properties. Herein, we report the colloidal synthesis of all‐inorganic low‐dimensional cesium copper halide nanocrystals (NCs) by adopting a hot‐injection approach. Using the same reactants and ligands, but different reaction temperatures, both 1D CsCu2I3 nanorods and 0D Cs3Cu2I5 NCs can be prepared. Density functional theory indicates that the reduced dimensionality in 1D CsCu2I3 compared to 0D Cs3Cu2I5 makes the excitons more localized, which accounts for the strong emission of 0D Cs3Cu2I5 NCs. Subsequent optical characterization reveals that the highly luminescent, strongly Stokes‐shifted broadband emission of 0D Cs3Cu2I5 NCs arises from the self‐trapped excitons. Our findings not only present a method to control the synthesis of low‐dimensional cesium copper halide nanocrystals but also highlight the potential of 0D Cs3Cu2I5 NCs in optoelectronics.
Single‐Crystal Cobalt Phosphate Nanosheets for Biomimetic Oxygen Evolution in Neutral Electrolytes
Authors: Shao Yue , Xiao Xue , Zhu Yun‐Pei , Ma Tian‐Yi 
Published: August 2019

AbstractTo improve energy conversion efficiency, the development of active electrocatalysts with similar structural features to photosynthesis II systems (PS‐II), which can efficiently catalyze the oxygen evolution reaction (OER), have received great research interest. Crystalline cobalt phosphate nanosheets are designed as an efficient OER catalyst in neutral media, showing outstanding performance that even outperforms the noble RuO2 benchmark. The correlation of experimental and computational results reveals that the active sites are the edge‐sharing CoO9 structural motif, akin to the molecular geometry of PS‐II. This unique structure can facilitate reaction intermediate adsorption and decrease the reaction energy barrier, thus improving the OER kinetics.
Magnesium Cyanide or Isocyanide?
Authors: Ballmann Gerd , Elsen Holger , Harder Sjoerd 
Published: August 2019

AbstractPreference for the binding mode of the CN− ligand to Mg (Mg−CN vs. Mg−NC) is investigated. A monomeric Mg complex with a terminal CN ligand was prepared using the dipyrromethene ligand MesDPM which successfully blocks dimerization. While reaction of (MesDPM)MgN(SiMe3)2 with Me3SiCN gave the coordination complex (MesDPM)MgN(SiMe3)2⋅NCSiMe3, reaction with (MesDPM)Mg(nBu) led to (MesDPM)MgNC⋅(THF)2. A Mg−NC/Mg−CN ratio of ≈95:5 was established by crystal‐structure determination and DFT calculations. IR studies show absorbances for CN stretching at 2085 cm−1 (Mg−NC) and 2162 cm−1 (Mg−CN) as confirmed by 13C labeling. In solution and in the solid state, the CN ligand rotates within the pocket. The calculated isomerization barrier is only 12.0 kcal mol−1 and the 13C NMR signal for CN decoalesces at −85 °C (Mg−NC: 175.9 ppm, Mg−CN: 144.3 ppm). Experiment and theory both indicate that Mg complexes with the CN− ligand should not be named cyanides but are more properly defined as isocyanides.
Transfer and Amplification of Chirality Within the “Ring of Fire” Observed in Resonance Raman Optical Activity Experiments
Authors: Li Guojie , Kessler Jiří , Cheramy Joseph , Wu Tao , Poopari Mohammad Reza , Bouř Petr , Xu Yunjie 
Published: August 2019

AbstractWe report extremely strong chirality transfer from a chiral nickel complex to solvent molecules detected as Raman optical activity (ROA). Electronic energies of the complex were in resonance with the excitation‐laser light. The phenomenon was observed for a wide range of achiral and chiral solvents. For chiral 2‐butanol, the induced ROA was even stronger than the natural one. The observations were related to so‐called quantum (molecular) plasmons that enable a strong chiral Rayleigh scattering of the resonating complex. According to a model presented here, the maximal induced ROA intensity occurs at a certain distance from the solute, in a three‐dimensional “ring of fire”, even after rotational averaging. Most experimental ROA signs and relative intensities could be reproduced. The effect might significantly increase the potential of ROA spectroscopy in bioimaging and sensitive detection of chiral molecules.