bluuebunny/crossref_metadata_2025_split huggingface dataset:
UV‐light and dietary vitamin D and their effects on ionized calcium and 25‐OH‐D plasma concentrations in captive gentoo penguins (Pygoscelis papua)
Authors: Tröndle Ursina , Steinmetz Hanspeter W. , Rüegg Simon R. , Müller Anja , Liesegang Annette 
Published: July 2018

AbstractIn this study, the effect of ultraviolet (UV) light and dietary vitamin D on calcium metabolism in permanently indoor‐housed gentoo penguins (Pygoscelis papua) was investigated. The study consisted of three periods, each completed with blood samples to analyse plasma concentrations of 25‐OH‐D, 1,25‐(OH)2‐D, ionized (iCa) and total calcium (tCa). During the first study period (D), animals were housed under routine conditions without UV‐light and fed a diet of different fish species, supplemented with 1,000 IU vitamin D per animal and day. The following study period (Baseline) of 28‐day duration consisted of the same diet without any vitamin D supplementation and without UV‐light. During the study period (UVB) artificial UV‐light was added for 3 weeks. The vitamin D content of fish was measured by high‐performance liquid chromatography. It varied between fish species and between facilities, ranging from no measurable content in capelin (Mallotus villosus) to 7,340 IU vitamin D/kg original matter (OM) in herring (Clupea spp). The average dietary vitamin D content was 311 IU/kg OM at facility 1 and 6,325 IU/kg OM at facility 2, resulting in a vitamin D intake per animal and day without supplementation of 130 IU (25.5 IU/kg body weight BW) and 2,454 IU (438.2 IU/kg BW) respectively. The supplementation of vitamin D elevated significantly the plasma concentrations of 25‐OH‐D by an intraindividual difference of 15 (range −2 to 59) nmol/L and tCa by 0.1 (0.0–0.3) mmol/L only at facility 2. The exposure to UV‐light raised the blood concentrations of tCa at facility 2 by 0.15 (0.1–0.2) mmol/L, and of iCa and tCa for females at facility 1 by 0.23 (0.13–0.41) mmol/L and 1.8 (1.1–2.5) mmol/L respectively. No significant influence of the study periods (D) and (UVB) was found for the concentrations of 1,25‐(OH)2‐D at both facilities.
Evaluation of factors associated with retained surgical sponges in veterinary patients: a survey of veterinary practitioners
Authors: Rodriguez F. R. , Kirby B. M. , Ryan J. 
Published: July 2018

ObjectivesTo evaluate factors that could be associated with retained surgical sponges in veterinary patients.Materials and MethodsA survey was distributed to 322 veterinarians attending a national veterinary conference in the UK. The survey included questions about the staff, scheduling, type of surgical procedure, surgical sponges, methods to track surgical sponges and details of clinical cases with retained surgical sponges.ResultsThe response rate was 64 of 322 (19%). Lack of designated scheduled time for surgical procedures was reported by 30% of respondents and was variable for 31%. More than half of respondents (66%) had two people involved in each surgical procedure. The majority of respondents sterilised their own surgical sponges (91%) and used non‐radiopaque surgical sponges (56%). Sponges were not counted by 27% of respondents and only occasionally by 20%. Sponge count was not recorded by 70% of respondents. The majority (66%) did not use or have a surgical checklist. Lack of awareness of gossypibomas was reported by 11% of respondents. In all, 27% of respondents were aware of at least one case of retained surgical sponge. Of the 17 cases reported, 14 were small animals. The abdomen was the most common anatomical location for retained surgical sponges and followed elective neutering.Clinical SignificanceDespite the low‐response rate, our results suggest that methods of surveillance might reduce the incidence of retained surgical sponges. Lack of specifically scheduled time for surgery, few theatre staff and lack of sponge counting and documentation may have contributed to the 17 retained surgical sponge cases reported.
Feasibility study of a combined lifestyle behaviors and asthma self‐management intervention for school‐aged children
Authors: Horner Sharon D. , Timmerman Gayle M. , McWilliams Bennie C. 
Published: July 2018

AbstractPurposeThe purpose of this study is to test the feasibility of delivering an intervention that combines healthy lifestyle behaviors related to weight management with asthma self‐management, the Living Healthy with Asthma intervention, to children who have asthma.Methods and DesignUsing a mixed design, the feasibility study of the 12‐week Living Healthy with Asthma intervention was conducted with a single group of children diagnosed with asthma. Pretest and posttest data were collected on asthma‐related (self‐management, metered dose inhaler [MDI] skill, asthma severity, quality of life [QOL]), and healthy lifestyle variables (body mass index [BMI], dietary quality). A matched comparison sample was drawn from a separate study that tested the same asthma self‐management component (single intervention) used in the feasibility study to determine if the Living Healthy with Asthma intervention worked as well as the single intervention for improving children's asthma self‐management.ResultsThirteen school‐aged children were enrolled in the feasibility study. There were significant reductions in BMI z‐scores (P = 0.007), and improvements in vegetable servings (P = 0.03), MDI skill (P = 0.005), children's QOL (P < 0.001), and parents’ QOL (P = 0.03). When comparing the feasibility group with the matched comparison group (n = 13), there were no significant differences in asthma self‐management, MDI skill, or asthma severity after the interventions.Practice ImplicationsFindings supported the feasibility of implementing the combined intervention, and it was not inferior to the single intervention—which supports nurses’ efforts to help families manage multiple health problems.
Comparative plasma and interstitial fluid pharmacokinetics and tissue residues of ceftiofur crystalline‐free acid in cattle with induced coliform mastitis
Authors: Gorden Patrick J. , Ydstie Joshua A. , Kleinhenz Michael D. , Brick Troy A. , Smith Joe S. , Griffith Ronald W. , Wulf Larry W. , Rajewski Suzanne M. , Zhang Min , Sidhu Pritam K. , Mochel Jonathan P. , Coetzee Johann F. 
Published: July 2018

AbstractCeftiofur (CEF) is a third‐generation cephalosporin that is the most widely used antimicrobial in the dairy industry. Currently, violative meat residues in cull dairy cattle are commonly associated with CEF. One potential cause for violative residues is altered pharmacokinetics of the drug due to disease, which could increase the time needed for the residue to deplete. The objectives of this study were (a) to determine the absolute bioavailability of CEF crystalline‐free acid (CFA) in healthy versus diseased cows; (b) to compare the plasma and interstitial fluid pharmacokinetics and plasma protein binding of CEF between healthy dairy cows and those with disease; and (c) to determine the CEF residue profile in tissues of diseased cows. For this trial, disease was induced through intramammaryEscherichia coliinfusion. Following disease induction and CEF CFA administration, for plasma concentrations, there was not a significant effect of treatment (p = 0.068), but the treatment‐by‐time interaction (p = 0.005) was significant. There was a significantly greater concentration of CEF in the plasma of the DIS cows at T2 hr (p = 0.002), T8 hr (p < 0.001), T12 hr (p = 0.001), and T16 hr (p = 0.002). For PK parameters in plasma, the slope of the terminal phase of the concentration versus time curve was significantly lower (p = 0.007), terminal half‐life was significantly longer (p = 0.014), and apparent volume of distribution during the elimination phase was significantly higher (p = 0.028) diseased group. There was no difference in plasma protein binding of CEF and interstitial fluid pharmacokinetics. None of the cows had kidney CEF residues above the US tolerance level following observation of the drug's withdrawal period, but one cow with a larger apparent volume of distribution and longer terminal half‐life had tissue residues slightly below the tolerance. Whereas these findings do not support the hypothesis that severely ill cows need longer withdrawal times, alterations in the terminal half‐life suggest that it is theoretically possible.
The presence of the brown bear Ursus arctos in Holocene Britain: a review of the evidence
Authors: O'Regan Hannah J. 
Published: July 2018

Abstract

The brown bear Ursus arctos was Holocene Britain's largest carnivoran and has appeared in recent rewilding discussions.

Despite widespread interest, we know very little about the species in Holocene Britain, as few studies have been undertaken. This paper draws together information on the brown bear to examine its presence and extinction through evaluation of the archaeological and palaeontological evidence.

Data were collected from published literature and museum catalogues. Information on the chronological date of archaeological sites, the number of bear specimens, and the body parts present were particularly sought.

A total of 85 sites were identified, 57 are well‐dated, 25 are of uncertain date but are likely to be Holocene, and three have uncertain species identifications. Very few specimens from non‐anthropogenic sites (e.g. fens and caves) are well‐dated, skewing the data towards anthropogenic sites such as settlements and graves.

Analysis of body part representation shows that the bear bones found in the Bronze Age, Iron Age, and most of the early medieval period are largely phalanges or metapodials, which are likely to be derived from skins. Other body parts are present during the Romano‐British, medieval, and post‐medieval period, indicating that live bears were present and were probably imported for entertainment.

It seems that the brown bear was rare in Britain throughout the Holocene, and, based on current evidence, two extinction scenarios can be put forward: extinction in the late Neolithic/early Bronze Age, or extinction in the early medieval period.
The reaction of carbonates in contact with laser‐generated, superheated silicate melts: Constraining impact metamorphism of carbonate‐bearing target rocks
Authors: Hamann Christopher , Bläsing Saskia , Hecht Lutz , Schäffer Sebastian , Deutsch Alex , Osterholz Jens , Lexow Bernd 
Published: July 2018

AbstractWe simulated entrainment of carbonates (calcite, dolomite) in silicate impact melts by 1‐bar laser melting of silicate–carbonate composite targets, using sandstone, basalt, calcite marble, limestone, dolomite marble, and iron meteorite as starting materials. We demonstrate that carbonate assimilation by silicate melts of variable composition is extremely fast (seconds to minutes), resulting in contamination of silicate melts with carbonate‐derived CaO and MgO and release of CO2 at the silicate melt–carbonate interface. We identify several processes, i.e., (1) decomposition of carbonates releases CO2 and produces residual oxides (CaO, MgO); (2) incorporation of residual oxides from proximally dissociating carbonates into silicate melts; (3) rapid back‐reactions between residual CaO and CO2 produce idiomorphic calcite crystallites and porous carbonate quench products; (4) high‐temperature reactions between Ca‐contaminated silicate melts and carbonates yield typical skarn minerals and residual oxide melts; (5) mixing and mingling between Ca‐ or Ca,Mg‐contaminated and Ca‐ or Ca,Mg‐normal silicate melts; (6) precipitation of Ca‐ or Ca,Mg‐rich silicates from contaminated silicate melts upon quenching. Our experiments reproduce many textural and compositional features of typical impact melts originating from silicate–carbonate targets. They reinforce hypotheses that thermal decomposition of carbonates, rapid back‐reactions between decomposition products, and incorporation of residual oxides into silicate impact melts are prevailing processes during impact melting of mixed silicate–carbonate targets. However, by comparing our results with previous studies and thermodynamic considerations on the phase diagrams of calcite and quartz, we envisage that carbonate impact melts are readily produced during adiabatic decompression from high shock pressure, but subsequently decompose due to heat influx from coexisting silicate impact melts or hot breccia components. Under certain circumstances, postshock conditions may favor production and conservation of carbonate impact melts. We conclude that the response of mixed carbonate–silicate targets to impact might involve melting and decomposition of carbonates, the dominant response being governed by a complex variety of factors.
Severe malnutrition in infants aged <6 months—Outcomes and risk factors in Bangladesh: A prospective cohort study
Authors: Munirul Islam M. , Arafat Yasir , Connell Nichola , Mothabbir Golam , McGrath Marie , Berkley James A. , Ahmed Tahmeed , Kerac Marko 
Published: July 2018

AbstractSevere acute malnutrition (SAM) affects ~4 million infants under 6 months (u6m) worldwide, but evidence underpinning their care is “very low” quality. To inform future research and policy, the objectives of our study were to identify risk factors for infant u6m SAM and describe the clinical and anthropometric outcomes of treatment with current management strategies. We conducted a prospective cohort study in infants u6m in Barisal district, Bangladesh. One group of 77 infants had SAM (weight‐for‐length Z‐score [WLZ] <−3 and/or bipedal oedema); 77 others were “non‐SAM” (WLZ ≥−2 to <+2, no oedema, mid‐upper‐arm circumference ≥125 mm). All were enrolled at 4–8 weeks of age and followed up at 6 months. Maternal education and satisfaction with breastfeeding were among factors associated with SAM. Duration of exclusive breastfeeding was shorter at enrolment (3·9 ± 2.1 vs. 5.7 ± 2.2 weeks, P < 0.0001) and at age 6 months (13.2 ± 8.9 vs. 17.4 ± 7.9 weeks; P = 0.003) among SAM infants. Despite referral, only 13 (17%) reported for inpatient care, and at 6 months, 18 (23%) infants with SAM still had SAM, and 3 (3.9%) died. In the non‐SAM group, one child developed SAM, and none died. We conclude that current treatment strategies have limited practical effectiveness: poor uptake of inpatient referral being the main reason. World Health Organization recommendations and other intervention strategies of outpatient‐focused care for malnourished but clinically stable infants u6m need to be tested. Breastfeeding support is likely central to future treatment strategies but may be insufficient alone. Better case definitions of nutritionally at‐risk infants are also needed.
Genomic parallelism and lack thereof in contrasting systems of three‐spined sticklebacks
Authors: Liu Shenglin , Ferchaud Anne‐Laure , Grønkjær Peter , Nygaard Rasmus , Hansen Michael M. 
Published: July 2018

AbstractParallel evolution and the extent to which it involves gene reuse have attracted much interest. Whereas it has theoretically been predicted under which circumstances gene reuse is expected, empirical studies that directly compare systems showing high and low parallelism are rare. Three‐spined stickleback (Gasterosteus aculeatus), where freshwater populations have been independently founded by ancestral marine populations, represent prime examples of phenotypic and genomic parallelism, but cases exist where parallelism is low. Based on RAD (restriction site associated DNA) sequencing, we analysed SNPs and chromosome inversions in populations in Denmark and Greenland showing low and high parallelism, respectively. We identified parallelism across freshwater populations in Greenland at genomic regions previously identified to be associated with marine–freshwater divergence. These same markers also separated Danish marine and freshwater sticklebacks, albeit to a weaker extent. Hence, parallelism was not absent in Denmark but possibly constrained by spatially and temporally varying selection. Divergence time estimates found one Danish freshwater population to be much older than the others. It also deviated strongly with respect to parallelism and may represent earlier postglacial colonization based on a different pool of standing variation and eliciting different adaptive responses to freshwater conditions. These findings provide empirical support to previous suggestions that the time since replicate populations had access to a common pool of standing variation is a major factor determining gene reuse. At last, based on the observed parallelism in the Greenlandic system we discuss the predictability of adaptive responses in newly established populations.
Urbanization as a facilitator of gene flow in a human health pest
Authors: Miles Lindsay S. , Johnson J. Chadwick , Dyer Rodney J. , Verrelli Brian C. 
Published: July 2018

AbstractUrban fragmentation can reduce gene flow that isolates populations, reduces genetic diversity and increases population differentiation, all of which have negative conservation implications. Alternatively, gene flow may actually be increased among urban areas consistent with an urban facilitation model. In fact, urban adapter pests are able to thrive in the urban environment and may be experiencing human‐mediated transport. Here, we used social network theory with a population genetic approach to investigate the impact of urbanization on genetic connectivity in the Western black widow spider, as an urban pest model of human health concern. We collected genomewide single nucleotide polymorphism variation from mitochondrial and nuclear double‐digest RAD (ddRAD) sequence data sets from 210 individuals sampled from 11 urban and 10 nonurban locales across its distribution of the Western United States. From urban and nonurban contrasts of population, phylogenetic, and network analyses, urban locales have higher within‐population genetic diversity, lower between‐population genetic differentiation and higher estimates of genetic connectivity. Social network analyses show that urban locales not only have more connections, but can act as hubs that drive connectivity among nonurban locales, which show signatures of historical isolation. These results are consistent with an urban facilitation model of gene flow and demonstrate the importance of sampling multiple cities and markers to identify the role that urbanization has had on larger spatial scales. As the urban landscape continues to grow, this approach will help determine what factors influence the spread and adaptation of pests, like the venomous black widow spider, in building policies for human and biodiversity health.
Speciation in sympatry with ongoing secondary gene flow and a potential olfactory trigger in a radiation of Cameroon cichlids
Authors: Poelstra Jelmer W. , Richards Emilie J. , Martin Christopher H. 
Published: July 2018

AbstractThe process of sympatric speciation in nature remains a fundamental unsolved problem. Cameroon crater lake cichlid radiations were long regarded as one of the most compelling examples; however, recent work showed that their origins were more complex than a single colonization event followed by isolation. Here, we performed a detailed investigation of the speciation history of a radiation ofCoptodoncichlids from Lake Ejagham, Cameroon, using whole‐genome sequencing data. The existence of the Lake EjaghamCoptodonradiation is remarkable as this 0.5 km2lake offers limited scope for divergence across a shallow depth gradient, disruptive selection is currently weak, and the species are sexually monochromatic. We infer that Lake Ejagham was colonized byCoptodoncichlids soon after its formation 9,000 years ago, yet speciation occurred only in the last 1,000–2,000 years. We show that secondary gene flow from a nearby riverine species has been ongoing, into ancestral as well as extant lineages, and we identify and date river‐to‐lake admixture blocks. One block contains a cluster of olfactory receptor genes that introgressed near the time of the first speciation event and coincides with a higher overall rate of admixture. Olfactory signalling is a key component of mate choice and species recognition in cichlids. A functional role for this introgression event is consistent with previous findings that sexual isolation appears much stronger than ecological isolation in EjaghamCoptodon. We conclude that speciation in this radiation took place in sympatry, yet may have benefited from ongoing riverine gene flow.
Pathway position constrains the evolution of an ecologically important pathway in aspens (Populus tremula L.)
Authors: Cole Christopher T. , Ingvarsson Pär K. 
Published: July 2018

AbstractMany ecological interactions of aspens and their relatives (Populus spp.) are affected by products of the phenylpropanoid pathway synthesizing condensed tannins (CTs), whose production involves trade‐offs with other ecologically important compounds and with growth. Genes of this pathway are candidates for investigating the role of selection on ecologically important, polygenic traits. We analysed sequences from 25 genes representing 10 steps of the CT synthesis pathway, which produces CTs used in defence and lignins used for growth, in 12 individuals of European aspen (Populus tremula). We compared these to homologs from P. trichocarpa, to a control set of 77 P. tremula genes, to genome‐wide resequencing data and to RNA‐seq expression levels, in order to identify signatures of selection distinct from those of demography. In Populus, pathway position exerts a strong influence on the evolution of these genes. Nonsynonymous diversity, divergence and allele frequency shifts (Tajima's D) were much lower than for synonymous measures. Expression levels were higher, and the direction of selection more negative, for upstream genes than for those downstream. Selective constraints act with increasing intensity on upstream genes, despite the presence of multiple paralogs in most gene families. Pleiotropy, expression level, flux control and codon bias appear to interact in determining levels and patterns of variation in genes of this pathway, whose products mediate a wide array of ecological interactions for this widely distributed species.
Inferring selection in instances of long‐range colonization: The Aleppo pine (Pinus halepensis) in the Mediterranean Basin
Authors: Ruiz Daniels Rose , Taylor Richard S. , Serra‐Varela María Jesús , Vendramin Giovanni G. , González‐Martínez Santiago C. , Grivet Delphine 
Published: July 2018

AbstractTeasing apart the effects of natural selection and demography on current allele frequencies is challenging, due to both processes leaving a similar molecular footprint. In particular, when attempting to identify selection in species that have undergone a recent range expansion, the increase in genetic drift at the edges of range expansions (“allele surfing”) can be a confounding factor. To address this potential issue, we first assess the long‐range colonization history of the Aleppo pine across the Mediterranean Basin, using molecular markers. We then look for single nucleotide polymorphisms (SNPs) involved in local adaptation using: (a) environmental correlation methods (bayenv2), focusing on bioclimatic variables important for the species’ adaptation (i.e., temperature, precipitation and water availability); and (b) FST‐related methods (pcadapt). To assess the rate of false positives caused by the allele surfing effect, these results are compared with results from simulated SNP data that mimics the species’ past range expansions and the effect of genetic drift, but with no selection. We find that the Aleppo pine shows a previously unsuspected complex genetic structure across its range, as well as evidence of selection acting on SNPs involved with the response to bioclimatic variables such as drought. This study uses an original approach to disentangle the confounding effects of drift and selection in range margin populations. It also contributes to the increased evidence that plant populations are able to adapt to new environments despite the expected accumulation of deleterious mutations that takes place during long‐range colonizations.