bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Protein Expression in Shewanella seohaensis Decolorizing Azo Dyes
Authors: de Souza Nadine Ana , Ramaiah Nagappa , Damare Samir , Furtado Bliss , Mohandass Chellandi , Patil Anushka , De Lima Marsha 
Published: July 2018

Background:Microbial remediation is an ecologically safe alternative to controlling environmental pollution caused by toxic aromatic compounds including azo dyes. Marine bacteria show excellent potential as agents of bioremediation. However, a lack of understanding of the entailing mechanisms of microbial degradation often restricts its wide-scale and effective application.Objective:To understand the changes in a bacterial proteome profile during azo dye decolorization.Methods:In this study, we tested a Gram-negative bacterium, Shewanella seohaensis NIODMS14 isolated from an estuarine environment and grown in three different azo dyes (Reactive Black 5 (RB5), Reactive Green 19 (RG19) and Reactive Red 120 (RR120)). The unlabeled bacterial protein samples extracted during the process of dye decolorization were subject to mass spectrometry. Relative protein quantification was determined by comparing the resultant MS/MS spectra for each protein.Results:Maximum dye decolorization of 98.31% for RB5, 91.49% for RG19 and 97.07% for RR120 at a concentration of 100 mg L-1 was observed. The liquid chromatography-mass spectrometry - Quadrupole Time of Flight (LCMS-QToF) analysis revealed that as many as 29 proteins were up-regulated by 7 hours of growth and 17 by 24 hours of growth. Notably, these were common across the decolorized solutions of all three azo dyes. In cultures challenged with the azo dyes, the major class of upregulated proteins was cellular oxidoreductases and an alkyl hydroperoxide reductase (SwissProt ID: A9KY42).Conclusion:The findings of this study on the bacterial proteome profiling during the azo dye decolorization process are used to highlight the up-regulation of important proteins that are involved in energy metabolism and oxido-reduction pathways. This has important implications in understanding the mechanism of azo dye decolorization by Shewanella seohaensis.
A Simple and Novel Colorimetric Optimized Assay for Determination of Hydrogen Peroxide Concentration Under Oxidative Stress Conditions
Authors: Masooleh Anahita K. , Ahmadikhah Asadollah 
Published: July 2018

Background:
Reactive oxygen species are formed through the electron transfer reactions in
the mitochondria and chloroplasts and rapidly converted to H2O2.Therefore, H2O2 as a more stable
ROS can be considered as an indicator of cellular stress and it can be used in a steady state to monitor
intracellular stress level. In this regard, the increasing use of various nanoparticles, most of which are
associated with biological systems, are essential to be studied for their possible adverse effects. We
measured the concentration of hydrogen peroxide in samples collected before and after the treatment
with silver nanoparticles by a novel method and optimized this method for the living tissue.

Methods:
In this study, we evaluated the endogenous H2O2 production from Pyricularia oryzae tissue
under normal and stress conditions (such as after treatment with nanoparticles) by spectrophotometric
assay. The method used is based on instant reaction of hydrogen peroxide with vanadium pentoxide in
sulfuric acid solution, forming a peroxovanadate complex that has a maximum absorption at 454 nm.
This method was also compared with other methods.

Results:
The results of this method compared with other methods in the same tissue showed that the
method is simple, inexpensive and more efficient, and the complex is stable for several hours and can
be used for a variety of H2O2 concentrations. Also, the detection range of the mentioned method
equals with high-sensitivity methods such as available commercial kits. Furthermore, this method can
also measure higher values of H2O2.

Conclusion:
The optimized methods for measuring the H2O2 concentration with vanadium pentoxide
in sulfuric acid solution by the colorimetric method are simple, efficient, rapid, accurate, cost-effective
and do not have problems of other methods. The measurements using this method in Pyricularia
oryzae under oxidative stress showed that the created oxidative stress caused by the use of silver
nanoparticles increased H2O2 in fungal tissue. H2O2 is the SOD reaction product that is later decomposed
by CAT. This method is able to measure H2O2 in different ranges and under normal and stress
conditions which are indicative of antioxidant defense. Therefore, we recommend it to the researchers
in similar conditions.
Optimized Trace Analysis of Verapamil in Biological Fluids Using Solvent Bar Micro Extraction Technique Coupled with HPLC-UV Detection
Authors: Farazmand Farzaneh , Qomi Mahnaz 
Published: July 2018

Introduction:Verapamil (Verap) is an antidysrhythmic agent and a calcium channel blocker, indicated for angina, hypertension, supraventricular arrhythmias, and migraine.Objective:Drug monitoring plays a critical role in patient survival. In order to prevent the onset of drug toxicity, trace levels of this drug should be determined.Methods:For this reason, solvent bar microextraction technique coupled with high-performance liquid chromatography was implemented.Results:Under optimum condition, verapamil was micro-extracted from a donor solution (pH=11) to an acceptor solution (pH=3.2). It was transferred through n-octanol as the organic solvent, which was impregnated in the pores of the hollow fiber. Salt addition (30%) had the major effect on the efficiency of the method. Interaction of time (65 min), temperature (25°C), and stirring rate (818 rpm) had a significant effect too. It all resulted in a limit of detection and quantification of 15 ng mL-1 and 50 ng mL-1, respectively. The relative standard deviations of analysis were 4.9% within a day (n=3) and 5.7% between days (n=9). The calibration curves represented good linearity for urine and plasma samples with coefficient estimations higher than 0.99 with a linearity range of 50-5000 ng mL-1. The relative standard deviation for intra- (n=3) and inter-(n=9) day was 4.2% and 5.7%, respectively.Conclusion:It could be concluded that the application of this method for dose monitoring can be done at hospital and healthcare facilities.
A Smart Green Spectrophotometric Method for Simultaneous Determination of Severely Overlapped Binary Mixtures Using Normalized Spectrum and Isosbestic Point as Resolving Tools
Authors: H. Mohamed Ekram , Hamza Ahmed , Reda Aya , Adel Ola , Atef Sara 
Published: July 2018

Background:
The ability of the normalized spectra when used as a divisor and in combination
with isosbestic point to resolve complex binary or ternary mixtures, Candesartan and Hydrochlorthiazide
binary mixture was taken as a model.

Introduction:
A green simple smart and accurate method using ethanol as a solvent namely simultaneous
derivative ratio (SIDD) was applied to prove the power of normalized spectra and isosbestic point as
spectrophotometric resolving tools.

Methods:
In the proposed SIDD method, the zero order spectra of drugs were simply manipulated using
the normalized spectra of CAN as divisor to obtain the ratio and first derivative spectra in two successive
steps. Firstly, the total amplitude at isosbestic point 255.4 nm of the ratio spectra of the mixture
was measured representing the total actual concentration of both drugs in the mixture. Then, the first
derivative of the ratio spectra was obtained to determine Hydrochlorothiazide concentration at 233 nm.
While the concentration of Candesartan was determined subsequently by subtracting the Hydrochlorothiazide
concentration calculated after derivatization from the total concentration of both drugs
obtained at the ratio spectra before the derivatization step.

Results:
The SIDD was successfully applied for simultaneous determination of both drugs in their pure
form or in their binary mixture either in synthetic prepared mixtures or in combined dosage form the
adopted method was validated according to the ICH guidelines and the results were found to be within
the acceptable limits.

Conclusion:
The adopted method highlighted the important role of normalized spectrum when used as a
divisor in addition to the importance of isosbestic point to resolve severely overlapped spectra. All the
measurements were carried using ethanol which is considered one of the greenest solvents making the
method an environmentally friendly one. the adopted method was validated according to the ICH guidelines
and the results were found to be within the acceptable limits.
Enhancing Antibacterial Activity by Combination of Chloramphenicol with Constituents from Dracaena cochinchinensis (Lour.) S.C.Chen
Authors: Ruga Ritbey , Chavasiri Warinthorn 
Published: July 2018

Background:
Seven compounds were isolated from Dracaena cochinchinensis and elucidated
their structure by NMR spectroscopic analysis and determined the optical rotation for certain
compounds.

Methods:
These compounds were screened for antibacterial activity by using agar well diffusion and determination
of minimum inhibitory concentration (MIC) was conducted by the broth micro-dilution method
using resazurin colorimetric assay. Further investigation on combination effect between each compound
with chloramphenicol was conducted by agar well diffusion method. Among the seven compounds, compound
4 displayed the highest antibacterial activity against P. acnes and S. aureus with the inhibition zone of
17.3 and 16.7 mm, respectively. The MIC value of 1 and 4 against all tested bacteria was 62.5 µM, whereas
those of other compounds were 62.5-250 µM. The combination of 4 and chloramphenicol exhibited the
most synergistic effect against P. acnes with the rate in increasing antibacterial activity of 4 in combination
as 1,250 folds. This compound also enhanced the antibacterial activity of chloramphenicol at 50 µM with an
inhibition zone of 16.3 mm comparing to its activity alone (8.7 mm).

Results & Conclusion:

The results revealed compound 4 displayed the highest antibacterial activity
alone and in combination with chloramphenicol against P. acnes and S. aureus.
Design, Synthesis and Biological Profiling of Novel Phenothiazine Derivatives as Potent Antitubercular Agents
Authors: Singh Sushil K. , Nath Gopal , Kumar Ashok , Sellamuthu Satheesh K. 
Published: July 2018

Background:
Neuroleptic phenothiazines have been reported for antitubercular activity, but
the unwanted side effect (antipsychotic activity) restricted their use as antitubercular drugs.

Objective:
The study aimed to carry out development of phenothiazine based antitubercular agents by
modifying/removing the chemical group(s)/ linker(s) of chlorpromazine essential for exerting an
antipsychotic effect.

Methods:
The designed molecules were filtered with a cut-off of docking score < 2.0 Kcal/mol against
dopamine receptors, so that their binding with the receptor would be reduced to produce no/ less antipsychotic
effect. The molecules were then synthesized and screened against M. tuberculosis H37Rv.
They were further screened against a gram-positive (S. aureus) and a gram-negative (E. coli) bacterial
strains to evaluate the spectrum of activity. The ability of the compounds to cross the blood-brain barrier
(BBB) was also analyzed. The compounds were further examined for cytotoxicity (CC50) against
mammalian VERO cells.

Results:
Compounds 14p, 15p and 16p were found to be the most effective against all the strains viz. M.
tuberculosis H37Rv, S. aureus and E. coli with MIC of 1.56µg/ml, 0.98µg/ml and 3.91µg/ml, respectively.
Further, BBB permeability was found to be diminished in comparison to chlorpromazine, which
would ultimately reduce the unwanted antipsychotic activity. They were also found to be free from toxicity
against VERO cells.

Conclusion:
The designed strategy, to enhance the antitubercular activity with concomitant reduction of
dopamine receptor binding and BBB permeability was proved to be fruitful.
PENGARUH LIKUIDITAS, SOLVABILITAS DAN PERPUTARAN MODAL KERJA TERHADAP PROFITABILITAS PADA PERUSAHAAN MANUFAKTUR YANG TERDAFTAR DI BURSA EFEK INDONESIA
Authors: Wahyuliza Suci , Dewita Nola 
Published: July 2018

<p><em>This study aims to determine the effect of liquidity, solvency and working capital turnover on profitability at manufacturing companies listed on the Indonesia Stock Exchange. In this study, the sample used as many as 32 companies. The data used in the form of financial statements listed on the Indonesia Stock Exchange. The data analysis technique used multiple linear regression analysis after passing the classical assumption test consisting of normality test, multicollinearity test, autocorrelation test and heteroscedasticity test. The results prove that liquidity has a significant effect on profitability, solvency has no effect on profitability and turnover of working capital has a significant positive effect on profitability. Of the two influential variables, liquidity has a greater impact than the working capital turnover. Simultaneously liquidity, solvency and turnover of working capital have a significant effect on profitability. The value of R2 Adjusted in this study is 0.159 which means that 15.9% profitability can be influenced by liquidity, solvency and working capital turnover. While the remaining 84.1% influenced by other variables.</em></p><p> </p><p>Penelitian ini bertujuan untuk mengetahui pengaruh likuiditas, solvabilitas dan perputaran modal kerja terhadap profitabilitas pada perusahaan manufaktur yang terdaftar di Bursa Efek Indonesia. Dalam penelitian ini, sampel yang digunakan sebanyak 32 perusahaan. Data yang digunakan berupa laporan keuangan yang terdaftar di Bursa Efek Indonesia. Teknik analisis data menggunakan analisis regresi linier berganda setelah melewati uji asumsi klasik yang terdiri dari uji normalitas, uji multikolinieritas, uji autokorelasi dan uji heteroskedastisitas. Hasil penelitian membuktikan bahwa likuiditas berpengaruh signifikan terhadap profitabilitas, solvabilitas tidak berpengaruh terhadap profitabilitas dan perputaran modal kerja berpengaruh positif signifikan terhadap profitabilitas. Dari kedua variable yang berpengaruh tersebut, likuiditas mempunyai pengaruh yang lebih besar dibandingkan dengan perputaran modal kerja. Secara simultan likuiditas, solvabilitas dan perputaran modal kerja berpengaruh signifikan terhadap profitabilitas. Nilai R<sup>2</sup> Adjusted dalam penelitian ini sebesar 0.159 yang berarti bahwa 15.9% profitabilitas dapat dipengaruhi oleh likuiditas, solvabilitas dan perputaran modal kerja. Sedangkan sisanya 84.1% dipengaruhi oleh variabel-variabel lain.</p>
ANALISIS SOSIALISASI PAJAK DAN PEMANFAATAN TEKNOLOGI INFORMASI TERHADAP KEPATUHAN PELAPORAN SPT WAJIB PAJAK DI KEPULAUAN RIAU
Authors: Nopiana Puspita Rama , Natalia Erni Yanti 
Published: July 2018

<p><em><span lang="EN">This research has purpose to analyze socialization of information technology utilization to taxpayer reporting compliance in Riau Islands either partially or simultaneously. This research method is descriptive with quantitative approach. The population of this study consists of the number of individual taxpayers registered in the Tax Office Pratama Batam, Tanjung Pinang and Bintan Islands Islands in Riau Islands amounting to 322,469 Taxpayers. The sample of the research is taxpayer as much as 298 respondents in Kepualauan Riau area, where the amount of sample of this research is calculated by using slovin formula. The results of the research conducted the test explains that: 1) tax socialization has a positive and significant effect for taxpayers on compliance reporting SPT, 2) utilization of information technology has a positive and significant impact for taxpayers on tax reporting compliance, 3) tax socialization and technology utilization the information simultaneously has a positive and significant influence for the taxpayer on tax reporting compliance, where the contribution of coefficient of determination (Adjusted R²) is 35,9% and the rest 64,1% is influenced by other factors not included in this research model.</span></em></p><p> </p><p>Penelitian ini memiliki tujuan untuk menganalisis sosialisasi pemanfaatan teknologi informasi terhadap kepatuhan pelaporan SPT Wajib Pajak di Kepulauan Riau baik secara parsial maupun simultan. Metode penelitian ini adalah deskriptif dengan pendekatan kuantitatif. Populasi penelitian ini terdiri dari jumlah Wajib Pajak orang pribadi yang terdaftar di Kantor Pelayanan Pajak Pratama Kota Batam, Kota Tanjung Pinang dan Kabupaten Bintan Kepulauan di Kepulauan Riau yang berjumlah 322.469 Wajib Pajak. Sampel penelitian adalah wajib pajak sebanyak 298 responden di wilayah Kepualauan Riau, dimana jumlah dari sampel penelitian ini dihitung dengan menggunkan rumus slovin. Hasil penelitian pengujian yang dilakukan menjelaskan bahwa: 1) sosialisasi pajak memiliki pengaruh positif dan signifikan bagi Wajib Pajak terhadap kepatuhan pelaporan SPT, 2) pemanfaatan teknologi informasi memiliki pengaruh positif dan signifikan bagi Wajib Pajak terhadap kepatuhan pelaporan SPT, 3) sosialisasi pajak dan pemanfaatan teknologi informasi secara simultan memiliki pengaruh positif dan signifikan bagi Wajib Pajak terhadap kepatuhan pelaporan SPT, dimana kontribusi nilai koefisien determinasi (Adjusted R²) sebesar 35,9% dan sisanya 64,1% dipengaruhi oleh faktor-faktor lainnya yang tidak dimasukan dalam model penelitian ini.</p>
CONTROLLING THE IMPLEMENTATION PRUDENTIAL PRINCIPLES IN BANKING LANDING BY FINANCIAL SERVICES AUTHORITY
Authors: Zaini Zulfi Diane , Hakim Lukmanul 
Published: July 2018

Sources of collection of banking funds collected from the community are then distributed to the community in the form of credit. If the amount of funds disbursed by the bank to the community through credit are not refundable in accordance with the term has been agreed, the credit quality can be classified as non-performing loans or often referred to as Non Performing Loan (NPL). The impact of high NPL levels is disrupted by the liquidity of each banking institution.Research Objectives are: (1) To analyze the supervision of the application of prudential principles in the provision of bank credit conducted by the Financial Services Authority under the Act Number 21 of 2011 on the Financial Services Authority. (2) To analyze the inhibiting factors in supervising the application of prudential principles in the provision of bank credit by the Financial Services Authority. Furthermore, this research uses research method with normative juridical approach, that is by collecting secondary data that is literature materials, as a technique to get information through tracing legislation and other regulations in accordance with research problems and then the data is analyzed by qualitative juridical .The results of the research show that (1) Supervision of prudential principles in the provision of bank credit conducted by the Financial Services Authority under the Act Number 21 of 2011 concerning Financial Services Authority shall be conducted by Compliance Based Supervision (CBS), that is Compliance Monitoring banks against provisions relating to the operation and management of banks in the past in order to ensure that the bank has been operating and managed properly and properly according to prudential principles. In addition, Risk Based Supervision (RBS) is implemented, ie supervision using risk-based strategies and methodologies that enable bank supervisors to detect significant risks early and take appropriate and timely monitoring actions. (2) Inhibiting factors in supervising the application of prudential principles in the provision of bank credit conducted by the Financial Services Authority is one of the most important that customers often in providing data to the Bank inaccurate and the existence of the prospective customer's delay in completing the file submission of credit. Keywords: Supervision, Financial Services Authority, Prudential Banking Principles, Credit
REVERSAL BURDEN OF EVIDANCE ON A CRIMINAL CORRUPTION IN INDONESIA
Authors: Hasnawati Hasnawati 
Published: July 2018

The research aim are to know the implementation of strict evidence in corruption committed in Indonesia and the constraint or obstacle faced by the corruption agency. The method of this research was normative juridical, because the research about reversal burden of proof of corruption in crime in Indonesia, which data obtained from primary, secondary, and tertiary legal materials. Research result reveal that implementation of proof in corruption cases is often felt to be ineffective and very burdensome to the Investigator apparatus therefor two theories of proof, namely the free theory embraced by the dependent and negative theory according to the law adopted by the public prosecutor or ordinary commonly called the theory reversal burden of limited proof and in balance , the dependent has the right to proved that he has not commited a criminal act of corruption and that the prosecutor still has the duty to verify the indictment. The obstacles faced by law enforcement in implementing the burdening system of proof on the handling of corruption crime, namely the Contraints for the public Prosecutor and the obstacle for the judge divided into 2(two) types, namely : Juridical and Non Juridical Contrains
PENGARUH KONSENTRASI GELATIN TULANG IKAN PATIN (Pangasius sp.) DAN KONSENTRASI SUSU SKIM TERHADAP KARAKTERISTIK ES KRIM UBI JALAR UNGU (Ipomoea batatas L.)
Authors: Velly Hervelly 
Published: July 2018

Tujuan dari penelitian ini adalah untuk menentukan konsentrasi gelatin tulang ikan patin (Pangasius hypotalamus) dan susu skim pada pembuatan es krim ubi jalar ungu. Manfaat dari penelitian ini adalah meningkatkan nilai guna dari tulang ikan, memanfaatkan tulang ikan patin untuk suatu produk dan diversifikasi pangan olahan ubi jalar ungu. Penelitian yang dilakukan terdiri dari dua tahap yaitu penelitian pendahuluan dan penelitian utama. Penelitian pendahuluan yang dilakukan adalah pembuatan gelatin tulang ikan patin dan membandingkan waktu leleh es krim menggunakan konsentrasi gelatin kering 0,3% dengan gelatin cair 0,3%. Penelitian utama yang dilakukan adalah membuat es krim ubi jalar ungu dengan menggunakan gelatin tulang ikan patin yang telah didapatkan dari penelitian pendahuluan dan menggunakan susu skim. Rancangan percobaan yang digunakan adalah pola faktorial 3x3 dalam Rancangan Acak Kelompok (RAK) yang terdiri dari 2 faktor yaitu faktor konsentrasi gelatin tulang ikan patin yang terdiri dari 3 taraf yaitu 0,1%, 0,3%, 0,5% dan faktor konsentrasi susu skim yang terdiri dari 3 taraf yaitu 8%, 10%, 12%. Respon yang dianalisis adalah kadar lemak, kadar protein, waktu leleh, overrun serta warna, rasa, aroma, tekstur es krim. Hasil dari penelitian pendahuluan menunjukkan gelatin kering tulang ikan patin mempunyai viskositas 65 mps, nilai pH 4,55, kekuatan gel 126,054 bloom dan rendemen 4,97%, waktu leleh es krim dengan konsentrasi gelatin tulang ikan patin kering 0,3% diperoleh 40,05 menit. Hasil penelitian utama menunjukan bahwa konsentrasi gelatin tulang ikan patin berpengaruh terhadap warna, aroma, rasa, overrun, waktu leleh dan protein es krim ubi jalar ungu, tetapi tidak berbeda nyata terhadap tekstur dan lemak. Konsentrasi susu skim berpengaruh terhadap warna, aroma, rasa, overrun dan waktu leleh es krim ubi jalar ungu, tetapi tidak berpengaruh terhadap kadar protein dan lemak. Interaksi antara konsentrasi gelatin tulang ikan patin dan konsentrasi susu skim berpengaruh terhadap rasa, aroma, waktu leleh dan overrun es krim ubi jalar ungu. Aktivitas antioksidan dari sampel terpilih dengan menggunakan metode DPPH diperoleh aktivitas antioksidan ubi mentah, ubi kukus dan es krim ubi ungu berturut-turut adalah 1684,76 ppm, 1825,17 ppm dan 1560,56 ppm