bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resection and repair of a Cas9 double-strand break at CTG trinucleotide repeats induces local and extensive chromosomal rearrangements
Authors: Mosbach Valentine , Viterbo David , Descorps-Declère Stéphane , Poggi Lucie , Vaysse-Zinkhöfer Wilhelm , Richard Guy-Franck 
Published: September 2019

SummaryMicrosatellites are short tandem repeats, ubiquitous in all eukaryotes and represent ∼2% of the human genome. Among them, trinucleotide repeats are responsible for more than two dozen neurological and developmental disorders. Targeting microsatellites with dedicated DNA endonucleases could become a viable option for patients affected with dramatic neurodegenerative disorders. Here, we used theStreptococcus pyogenesCas9 to induce a double-strand break within the expanded CTG repeat involved in myotonic dystrophy type 1, integrated in a yeast chromosome. Repair of this double-strand break generated unexpected large chromosomal rearrangements around the repeat tract. These rearrangements depended onRAD52,DNL4andSAE2, and both non-homologous end-joining and single-strand annealing pathways were involved. Resection and repair of the double-strand break (DSB) were totally abolished in arad50Δ strain, whereas they were impaired in asae2Δ mutant, only on the DSB end containing most of the repeat tract. This proved that Sae2 plays significant different roles in resecting a DSB end containing a repeated and structured sequence as compared to a non-repeated DSB end.In addition, we also discovered that gene conversion was less efficient when the DSB could be repaired using a homologous template, suggesting that the trinucleotide repeat may interfer with gene conversion too. Altogether, these data show thatSpCas9 is probably not a good choice when inducing a double-strand break at or near a microsatellite, especially in mammalian genomes that contain many more dispersed repeated elements than the yeast genome.
Quantifying normal and parkinsonian gait features from home movies: Practical application of a deep learning–based 2D pose estimator
Authors: Sato Kenichiro , Nagashima Yu , Mano Tatsuo , Iwata Atsushi , Toda Tatsushi 
Published: September 2019

AbstractObjectiveGait movies recorded in daily clinical practice are usually not filmed with specific devices, which prevents neurologists benefitting from leveraging gait analysis technologies. Here we propose a novel unsupervised approach to quantifying gait features and to extract cadence from normal and parkinsonian gait movies recorded with a home video camera by applying OpenPose, a deep learning–based 2D-pose estimator that can obtain joint coordinates from pictures or videos recorded with a monocular camera.MethodsOur proposed method consisted of two distinct phases: obtaining sequential gait features from movies by extracting body joint coordinates with OpenPose; and estimating cadence of periodic gait steps from the sequential gait features using the short-time pitch detection approach.ResultsThe cadence estimation of gait in its coronal plane (frontally viewed gait) as is frequently filmed in the daily clinical setting was successfully conducted in normal gait movies using the short-time autocorrelation function (ST-ACF). In cases of parkinsonian gait with prominent freezing of gait and involuntary oscillations, using ACF-based statistical distance metrics, we quantified the periodicity of each gait sequence; this metric clearly corresponded with the subjects’ baseline disease statuses.ConclusionThe proposed method allows us to analyze gait movies that have been underutilized to date in a completely data-driven manner, and might broaden the range of movies for which gait analyses can be conducted.
Commensalism in theMimiviridaegiant virus family
Authors: Jeudy Sandra , Bertaux Lionel , Alempic Jean-Marie , Lartigue Audrey , Legendre Matthieu , Belmudes Lucid , Santini Sébastien , Philippe Nadège , Beucher Laure , Biondi Emanuele G. , Juul Sissel , Turner Daniel J. , Couté Yohann , Claverie Jean-Michel , Abergel Chantal 
Published: September 2019

AbstractAcanthamoeba-infecting Mimiviridae belong to three clades: Mimiviruses (A), Moumouviruses (B) and Megaviruses (C). The uniquely complex mobilome of these giant viruses includes virophages and linear 7 kb-DNA molecules called “transpovirons”. We recently isolated a virophage (Zamilon vitis) and two transpovirons (maBtv and mvCtv) respectively associated to B-clade and C-clade mimiviruses. We used the capacity of the Zamilon virophage to replicate both on B-clade and C-clade host viruses to investigate the three partite interaction network governing the propagation of transpovirons. We found that the virophage could transfer both types of transpovirons to B-clade and C-clade host viruses provided they were devoid of a resident transpoviron (permissive effect). If not, only the resident transpoviron was replicated and propagated within the virophage progeny (dominance effect). Although B- and C-clade viruses devoid of transpoviron could replicate both maBtv and mvCtv, they did it with a lower efficiency across clades, suggesting an ongoing process of adaptive co-evolution. We performed proteomic comparisons of host viruses and virophage particles carrying or cleared of transpovirons in search of proteins involved in this adaptation process. These experiments revealed that transpoviron-encoded proteins are synthetized during the combined mimiviruses/virophage/transpoviron replication process and some of them are specifically incorporated into the virophage and giant virus particles together with the cognate transpoviron DNA. This study also highlights a unique example of intricate commensalism in the viral world, where the transpoviron uses the virophage to propagate from one host virus to another and where the Zamilon virophage and the transpoviron depend on their host giant virus to replicate, this without affecting the giant virus infectious cycle, at least in laboratory conditions.Author SummaryThe Mimiviridae are giant viruses with dsDNA genome up to 1.5 Mb. They build huge viral factories in the host cytoplasm in which the nuclear-like virus-encoded functions (transcription and replication) take place. They are themselves the target of infections by 20 kb-dsDNA virophages, replicating in the giant virus factories. They can also be found associated with 7kb-DNA episomes, dubbed transpovirons. We investigated the relationship between these three players by combining competition experiments involving the newly isolated Zamilon vitis virophage as a vehicle for transpovirons of different origins with proteomics analyses of virophage and giant virus particles. Our results suggest that relationship of the virophage, the transpoviron, and their host giant virus, extend the concept of commensalism to the viral world.
Ligand Entry into Fatty Acid Binding Protein via Local Unfolding instead of Gap Widening
Authors: Xiao T , Lu Y , Fan J , Yang D 
Published: September 2019

AbstractFatty acid binding proteins (FABPs) play an important role in transportation of fatty acids. Despite intensive studies, how fatty acids enter the protein cavity for binding is still controversial. Here, a gap-closed variant of human intestinal FABP was generated by mutagenesis, in which the gap is locked by a disulfide bridge. According to its structure determined here by NMR, this variant has no obvious openings as the ligand entrance and the gap cannot be widened by internal dynamics. Nevertheless, it still uptakes fatty acids and other ligands. NMR relaxation dispersion, chemical exchange saturation transfer and hydrogen-deuterium exchange experiments show that the variant exists in a major native state, two minor native-like state, and two locally unfolded states in aqueous solution. Local unfolding of either βB–βD or helix 2 can generate an opening large enough for ligands to enter the protein cavity, but only the fast local unfolding of helix 2 is relevant to the ligand entry process.Statement of SignificanceFatty acid binding proteins transport fatty acids to specific organelles in the cell. To enable the transport, fatty acids must enter and leave the protein cavity. In spite of many studies, how fatty acids enter the protein cavity remains controversial. Using mutagenesis and biophysical techniques, we have resolved the disagreement and further showed that local unfolding of the second helix can generate a transient opening to allow ligands to enter the protein cavity. Since lipid binding proteins are highly conserved in 3D structures and ligand binding, all of them may use the same local unfolding mechanism for ligand uptake and release.
Ease of product assembly through a time-based design methodology
Authors: Desai Anoop 
Published: September 2019

PurposeThis paper aims to present a design methodology to enable product design for ease of assembly. It is corroborated by means of a case study. The methodology is based on standard time data. This enables quick computation of assembly time as well as comparing different design options for ease of assembly.Design/methodology/approachComponent design that is easy to assemble is likely to take less time and vice versa. Assembly time is a function of product design attributes such as geometric shape, weight, center of gravity, type of material, number of fasteners and types of fasteners. The methodology uses standard data to achieve its objective. Numeric scores are developed for each design feature based on the aforementioned design attributes. This enables not only computation of assembly time for a brand new product but also comparison of two or more alternative design configurations from the point of view of ease of assembly.FindingsThe value of the system is corroborated by means of case studies of actual product designs. It is demonstrated that changing any of the underlying design attributes (such as type of fastener used, number of fasteners used, material of the component and component shape) is likely to result in changing the amount of time taken to assemble the product. The scoring system facilitates the quick computation of assembly timeOriginality/valueThe amount of time to assemble a product before the product is ever designed is facilitated by this system. Assembly time is a direct function of product design attributes. Process time is calculated using standard data, specifically, the Methods Time Measurement (MTM) system. This is accomplished by converting design features into time measurement units (TMUs). Assembly cost can then be easily computed by using assembly time as the basis. The computation of assembly time and cost is important inasmuch as its role in influencing productivity. This is of obvious value not only to the designer but the company as a whole.
Managers’ learning process during a health-promoting leadership intervention
Authors: Tjulin Åsa , Landstad Bodil , Vinberg Stig , Eriksson Andrea , Hagqvist Emma 
Published: September 2019

Purpose
The increasingly demanding psychosocial working conditions in Swedish public sector workplaces call for implementation of workplace health promotion (WHP) interventions. There is a need to increase first-line public sector managers’ capacities for health-promoting leadership. The purpose of this paper is to investigate first-line managers’ experiences of participating in an intervention aimed at strengthening health-promoting leadership. More precisely, the aim is to study what obstacles and prerequisites the intervention have for their learning processes to become health-promoting managers.

Design/methodology/approach
A qualitative study in Northern Sweden at workplaces in the county council and municipalities was conducted. The data were gathered through individual interviews with 18 participating first-line managers. Inductive-content analysis was used to analyse the data.

Findings
The results identify time for reflection and collegial discussions about leadership as prerequisites for learning about health-promoting leadership. Managers experienced the intervention as a confirmation of the leadership behaviours already gained. However, the health-promoting leadership intervention was seen as a contradiction, since organisational prerequisites to implement WHP measures were perceived to be lacking. The managers were not involved in the planning of the intervention and questioned why the organisation did not involve them more when the educational activities were created.

Originality/value
When the organisation understands how and when its managers learn, what they need and want to learn about WHP, and what they already know, tailored participatory interventions can be facilitated that consider the unique prerequisites for the particular organisation.
Numerical simulation of bubbly flow around a cylinder by semi-Lagrangian–Lagrangian method
Authors: Nguyen Van Luc , Degawa Tomohiro , Uchiyama Tomomi , Takamure Kotaro 
Published: September 2019

Purpose
The purpose of this study is to design numerical simulations of bubbly flow around a cylinder to better understand the characteristics of flow around a rigid obstacle.

Design/methodology/approach
The bubbly flow around a circular cylinder was numerically simulated using a semi-Lagrangian–Lagrangian method composed of a vortex-in-cell method for the liquid phase and a Lagrangian description of the gas phase. Additionally, a penalization method was applied to account for the cylinder inside the flow. The slip condition of the bubbles on the cylinder’s surface was enforced, and the outflow conditions were applied to the liquid flow at the far field.

Findings
The simulation clarified the characteristics of a bubbly flow around a circular cylinder. The bubbles were shown to move around and separate from both sides of the cylinder, because of entrainment by the liquid shear layers. Once the bubbly flow fully developed, the bubbles distributed into groups and were dispersed downstream of the cylinder. A three-dimensional vortex structure of various scales was also shown to form downstream, whereas a quasi-stable two-dimensional vortex structure was observed upstream. Overall, the proposed method captured the characteristics of a bubbly flow around a cylinder well.

Originality/value
A semi-Lagrangian–Lagrangian approach was applied to simulate a bubbly flow around a circular cylinder. The simulations provided the detail features of these flow phenomena.
Numerical investigation of heat transfer of laminar and turbulent pulsating Al2O3/water nanofluid flow
Authors: Hoseinzadeh S. , Heyns P.S. , Kariman H. 
Published: September 2019

Purpose
The purpose of this paper is to investigate the heat transfer of laminar and turbulent pulsating Al203/water nanofluid flow in a two-dimensional channel. In the laminar flow range, with increasing Reynolds number (Re), the velocity gradient is increased. Also, the Nusselt number (Nu) is increased, which causes increase in the overall heat transfer rate. Additionally, in the change of flow regime from laminar to turbulent, average thermal flux and pulsation range are increased. Also, the effect of different percentage of Al2O3/water nanofluid is investigated. The results show that the addition of nanofluids improve thermal performance in channel, but the using of nanofluid causes a pressure drop in the channel.

Design/methodology/approach
The pulsatile flow and heat transfer in a two-dimensional channel were investigated.

Findings
The numerical results show that the Al2O3/Water nanofluid has a significant effect on the thermal properties of the different flows (laminar and turbulent) and the average thermal flux and pulsation ranges are increased in the change of flow regime from laminar to turbulent. Also, the addition of nanofluid improves thermal performance in channels.

Originality/value
The originality of this work lies in proposing a numerical analysis of heat transfer of pulsating Al2O3/Water nanofluid flow -with different percentages- in the two-dimensional channel while the flow regime change from laminar to turbulent.
Abrasive wear analysis of RZ5/TiC in-situ composites: a statistical approach
Authors: Mehra Deepak , Mahapatra Manas Mohan , Harsha Suraj Prakash 
Published: September 2019

Purpose
The RZ5 mg alloy is used in automotive and aerospace applications including helicopter gearboxes and aircraft components. These components are prone to the wear as per the demands. The present work is the study of the significance of hard particle/ceramic, i.e. titanium carbide (TiC) in RZ5 mg alloy to protect the machine components from wear.

Design/methodology/approach
The abrasive wear analysis of in-situ RZ5-TiC magnesium matrix composite is considered for the study. The primary focus of the present work is to analyze the effects of varying control parameters, i.e. Wt.% of TiC, sliding distance and applied load on the responses, i.e. weight loss and coefficient of friction. Full factorial design of the experiment based on statistical analysis is used.

Findings
It is observed that the individually Wt.% of TiC and sliding distance show the comparatively significant effect on both responses. Similarly, the interaction between sliding distance and Wt.% of TiC indicated the considerable impact on weight loss. The regression equations are developed and validated for estimating responses. It is observed that the percentage errors are not appearing more than 10 per cent of responses. Therefore, the close agreement between measured and predicted values shows the adequacy of the model. The control factor is optimized using multi-response optimization. The variations of the order of 2.47 and 2.35 per cent in target value of the coefficient of friction and weight loss are achieved.

Originality/value
The current manuscript provides a detailed abrasive wear statistical analysis of RZ5-TiC composite. The influence of control parameters on the responses using the full factorial design, the main effect plots and interaction effects are presented.
The tribological performance of engineered micro-surface topography by picosecond laser on PEEK
Authors: Wang Meiling 
Published: September 2019

Purpose
The purpose of this study is to investigate the effect of engineered micro-structures on the tribological properties of metal-polyetheretherketone (PEEK) surface.

Design/methodology/approach
Circular dimples with diameters of 25 and 50 µm were designed and manufactured on PEEK plate specimens using picosecond laser. Reciprocating friction and wear tests on a ball-on-flat configuration were performed to evaluate the tribological properties of the designed micro-structures in dry contacts. The loading forces of 0.9 and 3 N were applied.

Findings
As a result, obvious fluctuations of coefficient of friction curve were observed in tribosystems consisting of non-textured and textured PEEK with circular dimples of 25 µm in diameter. GCr15 ball/textured PEEK plate specimens with circular dimples of 50 µm in diameter revealed a superior friction and wear property.

Originality/value
Different to the existing studies in which the tribopairs consist of hard bearing couples, this study investigated the tribological properties of the engineered micro-structures on the hard-on-soft bearing couples.
Development of e-tendering evaluation system for Nigerian public sector
Authors: Abdullahi Bello , Ibrahim Yahaya Makarfi , Ibrahim Ahmed , Bala Kabir 
Published: September 2019

Purpose The revolution brought about by the internet and the World Wide Web has led to the development of numerous e-Tendering systems for public sector tendering that have automated various aspects of the manual tendering processes that are known to experience numerous problems. However, one key area that has not been fully addressed is the automation of the evaluation of public tenders based on group decision-making. This paper presents part of the development of a Web-based e-tendering system called Nigerian Public Sector eTender (NPS-eTender) that automate the evaluation of public sector tenders based on group decision-making.

Design/methodology/approach The system was developed using object-oriented methodologies. Specifically, Ripple and unified process methodologies were adopted.

Findings The results of the system validation showed that NPS-eTender has an average rating of 74% with respect to correct and accurate modelling of the existing tendering domain and an average rating of 67.6% with respect to its potential to enhance the proficiency of public sector tendering in Nigeria. Based on the results of the validation, it can be concluded that the automation of the tender evaluation process can lead to a more proficient tendering process.

Originality/value This research has contributed to the development of an e-Tendering system for the public sector that supports the whole tendering lifecycle including the automation of evaluation of public tenders based on group decision-making.
Measures to improve the adoption of life cycle assessment in the South African construction industry
Authors: Kwofie Titus Ebenezer , Aigbavboa Clinton Ohis , Thwala Wellington Didibhuku 
Published: September 2019

Purpose
In spite of the urge among stakeholders to increase sustainability in the built environment, the South African Construction Industry (SACI) continues to suffer from low level of adoption of strategies such as life cycle assessment (LCA) to increase sustainable building practices in building and infrastructure delivery, hence the need to increase the adoption of sustainable concepts and sustainability practices is an emergent necessity. This study aims to identify the measures that can increase the adoption of LCA toward overcoming the practical difficulties, theoretical concerns and structural differences encountered in making gains in achieving sustainable practices in the SA construction industry.

Design/methodology/approach
The study adopted a deductive research design using a questionnaire survey with mean scores, Kruskal–Wallis and least square regression analysis done.

Findings
The study determined ten measures that can significantly influence about 88 per cent improvement in the success of LCA adopting in the SA industry. Also, there was a higher level of consensus in the findings which offers credence and good representation of the practical reality in the LCA adoption in South Africa.

Originality/value
These measures could be seen to embrace behavioural, social, technical and policy dimensions of LCA adoption. The findings are thus crucial in overcoming challenges to LCA methodologies in achieving sustainable building practices in the construction process in building and infrastructural delivery in SACI.