bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breast cancer cell‐derived exosomal miR‐20a‐5p promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1
Authors: Guo Ling , Zhu Ye , Li Liandi , Zhou Shufen , Yin Guohua , Yu Guanghao , Cui Hujun 
Published: August 2019

AbstractBone metastasis of breast cancer makes patients suffer from pain, fractures, spinal cord compression, and hypercalcemia, and is almost incurable. Although the mechanisms of bone metastasis in breast cancers have been studied intensively, novel specific target will be helpful to the development of new therapeutic strategy of breast cancer. Herein, we focused on the microRNA of tumor cell‐derived exosomes to investigate the communication between the bone microenvironment and tumor cells. The expression of miR‐20a‐5p in the primary murine bone marrow macrophages (BMMs), MCF‐10A, MCF‐7, and MDA‐MB‐231 cell lines, as well as the cell‐derived exosomes were assessed by qRT‐PCR. Transwell assays were used to evaluate the effects of miR‐20a‐5p on tumor cell migration and invasion. The expression of exosomes marker including CD63and TSG101 was detected by Western Blot. Cell cycle distribution of BMMs was analyzed by flow cytometry. 3‐UTR luciferase reporter assays were used to validate the putative binding between miR‐20a‐5p and SRCIN1. MiR‐20a‐5p was highly expressed in breast tumor tissues and the exosomes of MDA‐MB‐231 cells. MiR‐20a‐5p promoted migration and invasion in MDA‐MB‐231 cells, and the proliferation and differentiation of osteoclasts. MDA‐MB‐231 cell‐derived exosomes transferred miR‐20a‐5p to BMMs and facilitated the osteoclastogenesis via targeting SRCIN1. The present work provides evidence that miR‐20a‐5p transferred from breast cancer cell‐derived exosomes promotes the proliferation and differentiation of osteoclasts by targeting SRCIN1, providing scientific foundations for the development of exosome or miR‐20a‐5p targeted therapeutic intervention in breast cancer progression.
Everolimus‐containing therapy vs conventional therapy in the treatment of refractory breast cancer patients with PI3K/AKT/mTOR mutations: A retrospective study
Authors: Chen Zhanhong , Zheng Yabing , Cao Wenming , Zhang Yuzi , Zhao Zhengyi , Wang Guoqiang , Zhao Jing , Cai Shangli , Shao Xiying , Huang Jian , Ye Weiwu , Huang Yuan , Li Wei , Huang Xiang , Wu Hao , Wang Xiaojia , Yin Yongmei 
Published: August 2019

AbstractBackgroundPrevious case reports have shown the promising antitumor activity of everolimus in solid tumors containing molecular aberrations in PI3K/ATK/mTOR pathway, however, whether it is effective in patients with breast cancer remains unknown. Therefore, we conducted this retrospective cohort study to compare the efficacy of molecularly matched targeted therapy with everolimus to conventional therapy in refractory breast cancer patients harboring PI3K/ATK/mTOR pathway activating mutations.MethodsRefractory metastatic breast cancer patients who have received molecular screening using next‐generation sequencing (NGS) between September 8, 2015 and October 30, 2017 in two sites were screened for this study. The primary outcome was progression‐free survival (PFS). The secondary outcomes were overall response rate (ORR), disease control rate (DCR), and safety profile.ResultsA total of 78 patients were screened for analysis, amongst all, 52 (66.7%) had at least one gene mutation in PI3K/AKT/mTOR pathway. The most common mutation fell in PIK3CA (76.9%, 40/52) with a mutational prevalence of 51.3%. Of the 32 patients who were eligible for efficacy analysis, patients in the everolimus group (n = 19) exhibited shorter PFS than those in the conventional group (n = 13) (median, 1.9 vs 6.1 months; HR, 3.6; 95% CI, 1.48‐8.81; P = .0005). ORR was 15.4% (2/13) in the everolimus group and 23.1% (3/13) in the conventional group (P = 1.000), and DCR was 30.8% (4/13) and 100% (13/13) for each group, respectively (P = .000). The incidence of grade 3‐5 adverse events was relatively higher in the conventional group (38.5%, 5/13) than that in the everolimus group (26.3%, 5/19).ConclusionsOur findings suggested that everolimus might not be effective for cancer patients harboring mutations in PI3K/ATK/mTOR pathway and physicians should be cautious about its off‐label use in clinical practice.
Identification of a prosurvival neuroprotective mitochondrial peptide in a mammalian hibernator
Authors: Szereszewski Kama E. , Storey Kenneth B. 
Published: August 2019

AbstractHibernation requires the intricate regulation of physiological and biochemical adaptations to facilitate the decrease in metabolic rate and activation of prosurvival factors needed for winter survival. Mitochondria play important roles in eliciting these responses and in coordinating the required energy shifts. Herein, we report the presence of a novel mitochondrial peptide, s‐humanin, in the hibernating 13‐lined ground squirrel, Ictidomys tridecemlineatus. S‐humanin was shown to have strong structural and sequence similarities to its human analogue, humanin—a powerful neuroprotective mitochondrial peptide. An assessment of the protein and gene expression levels of this peptide in ground squirrels revealed stark tissue‐specific regulatory responses whereby transcript levels increased in brain cortex, skeletal muscle, and adipose tissues during hibernation, suggesting a protective torpor‐induced activation. Accompanying peptide measurements found that s‐humanin levels were suppressed in liver of torpid squirrels but enhanced in brain cortex. The enhanced transcript and protein levels of s‐humanin in brain cortex suggest that it is actively involved in protecting delicate brain tissues and neuronal connections from hibernation‐associated stresses. We propose that this squirrel‐specific peptide is involved in modulating tissue‐specific cytoprotective functions, expanding its role from human‐specific neuroprotection to environmental stress protection.Significance of the studyUnderstanding the molecular mechanisms, which protect against oxidative stress in a model hibernator such as the ground squirrel, could be pivotal to the regulation of cytoprotection. This study expands on our knowledge of metabolic rate depression and could suggest a potential role for humanin therapy in neurodegenerative diseases.
Improved Low‐Temperature Activity and H2O Resistance of Fe‐Doped Mn−Eu Catalysts for NO Removal by NH3−SCR
Authors: Guo Mingyu , Zhao Peipei , Liu Qingling , Liu Caixia , Han Jinfeng , Ji Na , Song Chunfeng , Ma Degang , Lu Xuebin , Liang Xingyu , Li Zhenguo 
Published: August 2019

AbstractNovel Mn−Eu−Fe catalysts were prepared via co‐precipitation method for NOx removal by selective catalytic reduction with NH3 (NH3−SCR). The Mn−Eu−Fe(1)‐500 catalyst possessed the highest level of low‐temperature activity, giving 98 % NO conversion at 100 °C. This catalyst also exhibited excellent H2O resistance capacity, even at a high gas hourly space velocity (GHSV) of 75,000 h−1. The NO conversion still maintained at approximately 90 % in the presence of 15 % H2O at 230 °C after 50 h, and the adverse effects of H2O could be quickly eliminated after the removal of H2O. Detailed characterization and analysis indicated that strong interactions among Mn, Eu and Fe resulted in the excellent low‐temperature SCR activity and H2O resistance of the Mn−Eu−Fe(1)‐500 catalyst. The strong interactions lead to larger specific surface area, higher concentrations of Mn4+, Fe3+ and Oα; improved redox properties, increased acid sites and acid strength, and more adsorption amounts of NO/NH3 in the presence of H2O. The Mn−Eu−Fe(1)‐500 catalyst is a potential future catalyst for low‐temperature NOx removal derived from high‐H2O‐content flue gases of natural gas combustion.
Introducing LNAzo: More Rigidity for Improved Photocontrol of Oligonucleotide Hybridization**
Authors: Grebenovsky Nikolai , Luma Larita , Müller Patricia , Heckel Alexander 
Published: August 2019

AbstractOligonucleotide‐based therapeutics have made rapid progress in clinical treatment of a variety of disease indications. Since most therapeutic oligonucleotides serve more than just one function and tend to have a prolonged lifetime, spatio‐temporal control of these functions would be desirable. Photoswitches like azobenzene have proven themselves as useful tools in this matter. Upon irradiation, the photoisomerization of the azobenzene moiety causes destabilization in adjacent base pairs, leading to a decreased hybridization affinity. Since the way the azobenzene is incorporated in the oligonucleotide is of utmost importance, we synthesized locked azobenzene C‐nucleosides and compared their photocontrol capabilities to established azobenzene C‐nucleosides in oligonucleotide test‐sequences by means of fluorescence‐, UV/Vis‐, and CD‐spectroscopy.
Stillstand reduzieren ‐ das Dauerthema
Authors: Hacker Manuela 
Published: August 2019

AbstractEs ist fast wie im echten Leben: unverhofft kommt oft und dann geballt. Bei (hoffentlich geplanten) Stillständen und dem anschließenden Anfahren von Anlagen warten immer wieder überraschungen auf die Mitarbeiter. Mal entdeckt man bei einem Stillstand eine bislang unbemerkte Unstimmigkeit, mal treten Probleme beim (Wieder‐)Anfahren der Anlage auf. Beides ist wenig erfreulich, liegt aber in der Natur der Sache und ist branchenübergreifend Routine in allen Betrieben. Wie man für derlei überraschungen vorsorgen kann, zeigt der Spezialist für Explosionsschutz und Druckentlastung Rembe.
Pflicht und Kür zugleich – die Technische Dokumentation
Authors: Erner Petra 
Published: August 2019

AbstractDie Meister Strömungstechnik erstellt seit zehn Jahren die Technischen Dokumentationen für ihre weltweit vertriebenen Produkte mit dem Redaktionssystem Cosima go!. Mit dieser Standardsoftware von Docufy erfüllen auch die Dokumentationen den hohen Qualitätsanspruch, für den das Unternehmen mit seinen Systemen zum Messen und überwachen von Durchfluss im industriellen Umfeld bekannt ist. Für Meister Strömungstechnik ist der Einsatz einer professionellen Dokumentations‐Software so wichtig wie ein ERP‐System.
Produktverunreinigung: Fehlanzeige
Authors: Meißler Sebastian 
Published: August 2019

AbstractDie Anforderungen an die Gebläsetechnik in der pneumatischen Förderung von Schüttgütern sind hoch. Das Anspruchsdenken beruht nicht nur auf der Tatsache, dass diese Systeme in vielen Industrien eine Schlüsselfunktion im Materialfluss einnehmen, sondern auch auf der Tatsache, dass die Druckluftqualität von entscheidender Bedeutung ist. Vor diesem Hintergrund hat die Aerzener Maschinenfabrik einen neuen und zertifizierten Sicherheitsstandard speziell für die Anforderungen in der Pneumatik entwickelt.
Wartungsarmer Betrieb im Atex‐Bereich
Authors: Wagner Petra 
Published: August 2019

AbstractZur alkoholbasierten Lösung von natürlichen Wirkstoffen aus Pflanzen sind mehrere Seitenkanalpumpen des Hamburger Pumpenherstellers Fristam im industriellen Einsatz. Die selbstansaugenden FZ 17 Kreiselpumpen fördern Alkohol aus IBCs in Tanks mit Pflanzenteilen, wie zum Beispiel Kamille. Der Alkohol wird solange immer wieder durch den Tank gepumpt, bis alle Wirkstoffe aus den Grundstoffen ausgelöst sind. Für diese Aufgabe und Produktionsumgebung sind die FZ‐Pumpen mit Magnetkupplung und Spalttopf hermetisch dicht abgeschlossen, besonders wartungsarm und erfordern weniger Atex‐konforme Peripherie als Pumpen mit Gleitringdichtungen.
Flexible Sekundärbarriere
Authors: Deininger Susanne 
Published: August 2019

AbstractIn Raffinerien und Tanklagern können durch Leckagen wassergefährdende und entflammbare Stoffe austreten. Sind die dafür vorgesehenen Auffangräume nicht mehr dicht, können diese Medien in den Boden eindringen. Eine Instandsetzung, insbesondere von kontaminierten Betonoberflächen, ist aufwändig und kostenintensiv. KTW Umweltschutztechnik bietet nun eine hochelastische Abdichtung auf einem Trägervlies an, welches auch auf kontaminierte Flächen eingebaut werden kann.
Retracted: How employee's perceived corporate social responsibility affects employee's pro‐environmental behaviour? The influence of organizational identification, corporate entrepreneurship, and environmental consciousness
Authors: Cheema Sadia , Afsar Bilal , Al‐Ghazali Basheer M. , Maqsoom Ahsen 
Published: August 2019

AbstractCorporate social responsibility (CSR) is a useful tool for effective organizational, social, and environmental functioning. This study expanded CSR and green research streams by examining the positive effect of employee's perceived CSR on pro‐environmental behaviour through mediation of organizational identification, as well as the moderation mechanisms of corporate entrepreneurship and employees' environmental consciousness for such an effect. The study drew on a survey sample of 479 employees and 122 department managers from various hotels in Pakistan. Key findings showed that CSR had both a direct and an indirect influence, through organizational identification, on pro‐environmental behaviour. The results lent support for the interactive effect of corporate entrepreneurship and environmental consciousness with CSR in predicting pro‐environmental behaviours. This is the first study of its kind to study a comprehensive model linking perceived CSR with employee's pro‐environmental behaviours in hotel industry through intervening variables.
Green Human Resource Management and Employee Green Behavior: An Empirical Analysis
Authors: Chaudhary Richa 
Published: August 2019

AbstractThis study was conducted with an objective to understand the role of green human resource management (GHRM) in fostering environmental performance of employee. Specifically, it examines the impact of GHRM practices on employee green performance behaviors (task related and voluntary) with organizational identification as a mediator and employee personal environmental values and gender as moderators. Three hundred one employee from automobile sector in India participated in the study. Using cross‐sectional research design, the proposed research model was tested with the help of hierarchical regression analysis. GHRM was found to significantly predict both task‐related and voluntary employee green behaviors. Organizational identification significantly mediated the effect, whereas gender and environmental values failed to moderate the relationship between GHRM and employee green behaviors. The study signifies the role of HRM in achieving environmental sustainability and emphasizes on the urgent need to embed sustainability dimension into HR systems to achieve sustainable development goals.
A Crosslinked Polyethyleneglycol Solid Electrolyte Dissolving Lithium Bis(trifluoromethylsulfonyl)imide for Rechargeable Lithium Batteries
Authors: Tian Guiying , Zhao Zijian , Zinkevich Tatiana , Elies Katharina , Scheiba Frieder , Ehrenberg Helmut 
Published: August 2019

AbstractReplacing liquid electrolytes with solid ones can provide advantages in safety, and all‐solid‐state batteries with solid electrolytes are proposed to solve the issue of the formation of lithium dendrites. In this study, a crosslinked polymer composite solid electrolyte was presented, which enabled the construction of lithium batteries with outstanding electrochemical behavior over long‐term cycling. The crosslinked polymeric host was synthesized through polymerization of the terminal amines of O,O‐bis(2‐aminopropyl) polypropylene glycol‐block‐polyethylene glycol‐block‐polypropylene glycol and terminal epoxy groups of bisphenol A diglycidyl ether at 90 °C and provided an amorphous matrix for Li+ dissolution. This composite solid electrolyte containing Li+ salt and garnet filler exhibited high flexibility, which supported the formation of favorable interfaces with the active materials, and possessed enough mechanical strength to suppress the penetration of lithium dendrites. Ionic conductivities higher than 5.0×10−4 S cm−1 above 45 °C were obtained as well as a wide electrochemical stability window (>4.51 V vs. Li/Li+) and a high Li+ diffusion coefficient (≈16.6×10−13 m2 s−1). High cycling stability (>500 cycles or 1000 h) was demonstrated.
On the impact of relay circuit losses in four‐coil wireless power transfer systems
Authors: de Miranda Caio M. , Pichorim Sergio F. , Abatti Paulo J. 
Published: August 2019

SummaryIn wireless power transfer (WPT) systems with more than two coils, the intermediary or relay circuits are used to extend the link distance. Thus, to achieve this extension efficiently in terms of power transfer, these relay circuits must have low losses. However, there are several instances in which there are restrictions in reducing the ohmic losses in all the relay circuits of the system. This is the case of biomedical applications where commonly there are size and access restrictions since one of the circuits can be implanted and also in applications using high‐temperature superconductor (HTS) coils due to the difficulty in implementing the necessary cooling system for all the coils of the system. Therefore, in these situations, the designer need to choose which relay circuit will be optimized. In this work, it is presented an analysis on the impact that losses in individual relay circuits have on efficiency, and power transfer, of typical four‐coil wireless power transfer systems consisting of circuit 1 (transmitter), relay circuits 2 and 3, and circuit 4 (load). It is shown that the losses on relay circuit 2 have greater impact on efficiency, while the losses of relay circuit 3 have a greater impact on power transfer for a given condition. Practical experiments confirm the developed analysis.
Improving time integration scheme for FEM analysis of MTL problems
Authors: Juric‐Grgic Ivica , Lucic Rino , Krolo Ivan 
Published: August 2019

SUMMARYThis paper presents improved algorithm for electromagnetic transient calculations on multiconductor transmission line (MTL) achieved by improvement of the time integration when forming a local system of equations for a finite element. Improvement of accuracy was obtained by using Heun's method. Previously developed local system of equations of MTL has been obtained using the generalized trapezoidal rule (ϑ‐method). The use of the generalized trapezoidal rule for the time integration sometimes causes numerical oscillations or numerical diffusion of numerical solutions. Numerical solutions obtained using Heun's method and using the generalized trapezoidal rule for different values of a time integration parameter are compared with analytical solution. It has been shown that Heun's method yields the results with much higher accuracy comparing to results obtained by generalized trapezoidal rule.
Hidden Secrets Behind Dots: Improved Phytoplankton Taxonomic Resolution Using High‐Throughput Imaging Flow Cytometry
Authors: Dunker Susanne 
Published: August 2019

AbstractPhytoplankton are aquatic, microscopically small primary producers, accounting for almost half of the worldwide carbon fixation. As early indicators of environmental change, they play a crucial role in water quality management. Human activities like climate change, eutrophication, or international shipping traffic strongly impact diversity of these organisms. Phytoplankton monitoring is a crucial step in the recognition of changes in community composition. The common standard for monitoring programs is manual microscopic counting, which strongly limits sample number and sampling frequency. In contrast, high‐throughput technologies like standard flow cytometry (FCM) are restricted to a low taxonomic resolution, which makes them unsuitable for the identification of indicator species. Imaging flow cytometers (IFC) could overcome these limitations as they combine microscopy and high‐throughput analysis. In comparison to single fluorescence values, image information not only allows for a wide variety of possibilities to characterize different species as well as immediate and fast measurements but also provides an archivable data output. Taxonomic resolution of IFC (ImageStream X Mk II) was proven comparable to standard FCM (FACSAria II) by the help of numerical evaluations. This is demonstrated on different levels of taxonomic differentiation of laboratory grown cultures in this study. Phytoplankton species discrimination by an imaging flow cytometer could be useful as supportive tool to make machine‐learning classifications more robust, reliable, and flexible. Furthermore, this study provides examples, demonstrating the possibility of discrimination between species with similar fluorescence properties, strains, and even subpopulations. In contrast to standard FCM, each cell is not only represented as a dot in a cytogram but is also linked to microscopic brightfield and the author presents a new way to visualize this as image‐based cytograms. The source code is supplied and could be useful for all kind of IFC data in general. © 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
Anterior trunk muscle shows mix of axial and appendicular developmental patterns
Authors: Sagarin Kathleen A. , Redgrave Anna C. , Mosimann Christian , Burke Ann C. , Devoto Stephen H. 
Published: August 2019

AbstractBackgroundSkeletal muscle in the trunk derives from the somites, paired segments of paraxial mesoderm. Whereas axial musculature develops within the somite, appendicular muscle develops following migration of muscle precursors into lateral plate mesoderm. The development of muscles bridging axial and appendicular systems appears mixed.ResultsWe examine development of three migratory muscle precursor‐derived muscles in zebrafish: the sternohyoideus (SH), pectoral fin (PF), and posterior hypaxial (PHM) muscles. We show there is an anterior to posterior gradient to the developmental gene expression and maturation of these three muscles. SH muscle precursors exhibit a long delay between migration and differentiation, PF muscle precursors exhibit a moderate delay in differentiation, and PHM muscle precursors show virtually no delay between migration and differentiation. Using lineage tracing, we show that lateral plate contribution to the PHM muscle is minor, unlike its known extensive contribution to the PF muscle and absence in the ventral extension of axial musculature.ConclusionsWe propose that PHM development is intermediate between a migratory muscle mode and an axial muscle mode of development, wherein the PHM differentiates after a very short migration of its precursors and becomes more anterior primarily by elongation of differentiated muscle fibers.
Yes‐associated protein expression in germ cells is dispensable for spermatogenesis in mice
Authors: Abou Nader Nour , Levasseur Adrien , Zhang Xiangfan , Boerboom Derek , Nagano Makoto C. , Boyer Alexandre 
Published: August 2019

SummaryYes‐associated protein (YAP), a key effector of the Hippo signaling pathway, is expressed in the nucleus of spermatogonia in mice, suggesting a potential role in spermatogenesis. Here, we report the generation of a conditional knockout mouse model (Yapflox/flox; Ddx4cre/+) that specifically inactivates Yap in the germ cells. The inactivation of Yap in spermatogonia was found to be highly efficient in this model. The loss of Yap in the germ cells had no observable effect on spermatogenesis in vivo. Histological examination of the testes showed no structural differences between mutant animals and age‐matched Yapflox/flox controls, nor was any differences detected in gonadosomatic index, expression of germ cell markers or sperm counts. Cluster‐forming assay using undifferentiated spermatogonia, including spermatogonial stem cells (SSCs), also showed that YAP is dispensable for SSC cluster formation in vitro. However, an increase in the expression of spermatogenesis and oogenesis basic helix–loop–helix 1 (Sohlh1) and neurogenin 3 (Ngn3) was observed in clusters derived from Yapflox/flox; Ddx4cre/+ animals. Taken together, these results suggest that YAP fine‐tunes the expression of genes associated with spermatogonial fate commitment, but that its loss is not sufficient to alter spermatogenesis in vivo.