bluuebunny/crossref_metadata_2025_split huggingface dataset:
PLASMID-INDUCED ANTIBIOTIC RESISTANCE AMONG PATHOGENS OF CHRONIC PYELONEPHRITIS: PREVALENCE OF GENES AND MAJOR RISK FACTORS
Authors: Chub О. , Bilchenko O. 
Published: June 2018

The aim of the study is to determine the prevalence of plasmid-mediated resistance genes among uro- pathogens from hospitalized patients with chronic pyelonephritis.
Methods. A cross-sectional study of 105patients with chronic pyelonephritis and different stage of chronic kidney disease, was carried. Screening for the presence ofplasmid-mediated genes was performed by polymerase chain reaction. Determining the risk factors was performed by analysis of prevalence Odd-ratio.
Results. The prevalence of plasmid-mediated resistance mechanisms among uropathogens is 36.7%, mainly due to extended-spectrum p-lactamase (25%). The main factors related with appearance of plasmid-mediated resistance genes were age range above 55 years (OR 3.05), hypertension (OR 2.57), Chronic Kidney Disease stage ІІІ (OR 2,03) and V (OR 1,1), in-patient treatment history (OR 2.02), duration of CP more than 10 years (OR 1,97), history of using antibiotics last year (OR 1,41).
Conclusion. Isolation and detection of plasmid-mediated resistance mechanisms among urinary strains are essential for the selection of the most effective antibiotic for the empiric treatment.
ESTIMATION OF NEPHROPROTECTIVE EFFECT OF ANGIOTENSIN-CONVERTING ENZYME RAMIPRIL IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS
Authors: Dolinnayä M.A. , Shekhovtseva T.G. 
Published: June 2018

Цель: изучить взаимосвязь между морфологическими показателями гломерулярного поражения почек и цистатином С (CysC) сыворотки крови у больных хроническим гломерулонефритом (ХТН) с сохраненной функцией почек, а также оценить терапевтическую коррекцию выявленных нарушений с помощью и АПФ рамиприла.
Материалы и методы исследования. Обследован 81 больной с ХТН. Пациенты были разделены на 2 клинические группы: больные ХТН с артериальной гипертензией (АТ), ХТН без АТ. Средняя суточная доза рамиприла у больных ХТН с АТ составила 12,8 ± 5,6 мг, у пациентов с ХТН без АТ – 2,5 мг.
Для анализа гломерулярного поражения почек у больных ХТН использовались данные нефробиопсий по следующим параметрам: пролиферация мезангиальных клеток, расширение мезангиального матрикса, гломерулосклероз, наличие фиброзных полумесяцев, утолщение капиллярных петель и/или их сращение с капсулой Боумена, утолщение и/или расщепление гломерулярной базальной мембраны, наличие экстракапиллярного компонента в просвете капсулы Боумена. Уровень Cys C в сыворотке крови определяли иммуноферментным методом.
Результаты и обсуждение. Нами статистически подтверждена прямая связь между cysC крови и гло- мерулосклерозом (r = 0,85; р < 0,05). Установлено, что метод диагностики гломерулосклероза почек с помощью определения маркера cysC в сыворотке крови является высокочувствительным и специфичным, с эффективностью 96,55%.
Выводы: сывороточный уровень cys C является чувствительным маркером гломерулосклероза почек у больных ХТН с диагностической эффективностью до 97%. Под влиянием 24-недельной терапии и АПФ рамиприлом у больных ХТН с наличием АТ и без наблюдается достоверное снижение маркеров поражения почек, в том числе и cysC сыворотки крови, что подтверждает нефропротекторное действие препарата.
FACTORS ASSOCIATED WITH INTRADIALYTIC HYPOTENSION IN HEMODIALYSIS PATIENTS AND WAYS OF ITS CORRECTION. CLINIC EXPERIENCE
Authors: Snisar L. , Liksunova L. , Aleksieieva N. 
Published: June 2018

Results of studies in recent years show a worse survival rate of dialysis patients who have frequent episodes intradialytic hypotension (IDH), as opposed patients without IDH.
The purpose of this quality improvement project was to study the factors associated with intradialytic hypotension in these patients and institute appropriate measures to mitigate this issue.
Patients and methods. In our clinic, we identified that 14.3% of dialysis patients experienced a decrease in their systolic blood pressure (SBP) to below 80 during dialysis.
Results. The results were studied for 3 months. We found that 57.1% of patients experienced an improvement in their blood pressure profile over the period of the study.
Conclusions. Factors associated with IDH are hypocalcemia, heart failure with systolic dysfunction, increase mterdialytic body weight over 2.5 kg and hospitalizations more than 2 times/year. Suggesting that simple changes to dialysis prescription can result in a significant reduction in the incidence of IDH.
THE ASSOCIATION OF DYSLIPIDEMIA WITH INTRAPERITONEAL INFLAMMATION AND PERITONEAL DIALYSIS TECHNIQUE SURVIVAL
Authors: Stepanova N. , Burdeyna O. , Dudar I. , Driyanska V. , Snisar L. , Shifris I. , Krasyuk E. , Shimova A. 
Published: June 2018

The aim of the study was to determine the relationship between the dyslipidemia, intraperitoneal inflammation and peritoneal dialysis (PD) survival.
Patients and methods. A total of 40patients with end-stage renal disease treated with continuous ambulatory peritoneal dialysis (PD) have been included in a prospective, observational study (average age was 49.3 ± 12.7). All patients were determined the blood lipid spectrum and IL-10, TNF-a, MCP-1 levels in peritoneal dialysis effluent (PDE). PD adequacy indicators evaluated by determining the concentration of urea and creatinine in plasma, urine and dialysate, calculated weekly creatinine clearance (CrCl), dialysis (Kt/ Vd), renal (Kt/ Vr) and total weekly urea clearance (Kt/ V).
Results. Dyslipidemia defined as increase of atherogenic lipoprotein fractions and inhibition ofHDL cholesterol was identified in 70% of the PD-patients. LDL cholesterol level and, accordingly, an atherogenic index (AI) were significantly dependent on the duration of PD treatment (R2 = 2.18 ± 0.15 (95% CI 1.87, 2.5), p < 0,0001 and R2 = 2.77 ± 0.27(95% CI 2.2, 3.3), p < 0,0001). The blood levels of total cholesterol, LDL-C and TG in the patients with diabetes were significantly higher compared with the diabetes-free patients (p = 0.007, p = 0.001 andp = 0.02, respectively).
Reducing the HDL cholesterol level was associated with high intraperitoneal production of pro-inflammatory mediators TNF-a (r = - 0.53;p = 0.001) and anti-inflammatory IL-10 (r = - 0.783;p <0.0001), whereas hypertriglyceridemia and high VLDL cholesterol are associated with an increase of MCP-1 (r = 0.6;p = 0.0003 and r = 0.55;p = 0.002, respectively).
The Cox proportional hazards regression analysis demonstrated the significant effect of VLDL cholesterol, LDL cholesterol and AI on the survival of PD technique survival (x2 = 24.8; p < 0.0001). The results of the Kaplan–Meier analysis and log-rank test also identified that there was a significant difference in the cumulative technical survival rate between the patients with AI level < 3.5 and > 3.5 (log-rank test: x2 = 19.8, P = 0.001.
Conclusions. Our results can be considered dyslipidemia in PD-patients not only as a traditional risk factor for CVD, but also as a predictor of chronic intraperitoneal inflammation and decrease of PD technical survival.
INFLUENCE OF GLICEROL ON FUNCTIONAL-MORPHOLOGICAL INDICATORS OF KIDNEYS AT MODELLING RENAL INSUFFICIENCY IN RATS
Authors: Kondakov I. I. , Topchii I. I. , Kirienko O. M. 
Published: June 2018

It is known, that after acute damage of kidney there is a risk of development of chronic kidney desease and death rate from its complications.
Material and methods. Research are spent on 40 not purebred rats at modelling of acute damage of the kidneys caused by toxic doses of glycerol. To evaluate histopathologal changes , we used cuts of fabrics painted gematoksilin- eozinom. Ultrathin preparations analyzed in electronic microscope PЕМ-125К.
Results. Administration of glycerol leads to uremia with dystrophic changes in tubular device and glomerular hypertrophy. At a stage of chronic renal insufficiency reduction of the glomerular sizes, tubulointerstitial fibrosis and glomerulosclerosis is observed.
Conclusion. At modelling of a nephritic pathology by introduction of 50 % glycerol infringement of function of kidneys has phasic character with probable development of chronic kidney desease in 8 weeks.
A SURVIVAL OF PATIENTS IN MAINTENCE HEMODIALYSIS DEPENDING ON EXPRESSED ANAEMIA AND CHARACTER OF DEFEAT OF KIDNEY
Authors: Savchuk V. 
Published: June 2018

The aim of the present research was to study the survival of hemodialysis (HD) patients depending on of anemia and character of kidney damage, «hemoglobin variability”
Materials and methods: We have conducted a retrospective study of 183 hemodialysis patients who were treated from 2009. to 2012. The starting point of observation was defined the first hemodialysis session. The patients were divided in accordance of hemoglobin (Hb) levels for 4 groups: the level of Hb <70 g/l (n=32) – group I, Hb 70–89 g/l (n=68) - group II, Hb 90-110 g/l (n=65) – group III, Hb≥110g/l (n=28) - IV group.
Results. The cumulative survival of patients was 55% at the moment of the end of the study. The cumulative proportion surviving of patients with diabetes mellitus was significantly lower compared to non-diabetic patients – 18% and 57%, respectively.
The survival of patients I & II groups was significantly lower than the patients of groups III & IV: 83.64% and 63.75% versus 31.7% and 29.28%, respectively (criteria Geha-Wilcoxon, Cox-Mantel test, Wilcoxon-Peto, F-criterion Cox log-rank test).
The survival of patients with "stable hemoglobin" was significantly higher than in patients with "low of hemoglobin variability " and "high of hemoglobin variability"(р<0,005).
Conclusions. Hb level and its variability had a significant effect on the survival of patients who are treated with hemodialysis. The survival of hemodialysis patients with diabetes mellitus was significantly lower than in non-diabetic kidney failure.
ANTIOXIDANT EFFECTOF NUCLEINAT SODIUM IN PATIENTS WITH CHRONIC KIDNEYDISEASE І–II STAGES: UNCOMPLICATED PYELONEPHRITIS
Authors: Stepanova N. , Korol L. , Migal L. , Romanenko O. 
Published: June 2018

Objective: to determine the antioxidant effects of Nucleinat sodium in patients with uncomplicated recurrent pyelonephritis.
Material and methods. We have analyzed the indicators of oxidant/ antioxidant (O/ A) balance in the blood of 55 patients with uncomplicated recurrent pyelonephritis. Women were randomized into 2 groups: I (n=30) consisted of patients treated with Nucleinat sodium at a dose of 0.25 mg 4 times daily for 14 days to II (n=25) included patients who used only antibacterial therapy (according to the set sensitivity identified pathogens). The control group consisted of 20 conditional healthy donors, matched for sex and age.
Results. In the blood ofpatients prior to treatment were observed reduction of total peroxidase activity of red blood cells (p<0.0001), ceruloplasmin (p=0,03), SH–groups (p=0.001) and increase in serum MDA, and oxidative stress index (p<0.0001) compared with the conditionally healthy donors. Comprehensive treatment with Nucleinat sodium statistically significantly led to normalization of these parameters and decrease of urine N–acetyl–p–D– glucosaminidase and p–galactosidase (p<0,001 and p=0.04, respectively).
Conclusions. For the treatment of uncomplicated recurrent pyelonephritis appropriate to apply Nucleinat sodium at a dose of 0.25 mg 4 times / day, which helps to reduce the activity of oxidative processes and the negative impact of oxidative stress on the patient, and increases of antioxidant protection.
HYPEROXALURIA AND BIOMARKERS OF MUCOSAL IMMUNITY IN PATIENTS WITH RECURRENT PYELONEPHRITIS
Authors: Kolesnyk M. , Stashevska N. , Stepanova N. , Dryyanskaya V. , Rudenko A. , Kruglykov V. , Kornylina O. 
Published: June 2018

Summary: The aim of our study was to compare the performance of mucosal immunity in urine and saliva of patients with chronic recurrent pyelonephritis subject to availability of hyperoxaluria.
Material and methods. To observational cross–sectional study included 40 women with chronic recurrent pyelonephritis, aged 21 to 48 years (31.6±7.7). Depending on the availability hyperoxaluria (oxalate excretion in the urine than 0.45 mmol per day) patients were divided into II Groups: for I (n=29) included women with hyperoxaluria, to II (n=11) – includes patients with normal excretion oxalate (7.2±2.4 vs 43.8±5.2; p <0.001). State of mucosal immunity was assessed by determining the content of lysozyme, lactoferrin, secretory immunoglobulin A (sIg A) and tumor necrosis factor alpha (TNF– a) in urine samples and content sIg A and class antibodies sIg A to lipopolysaccharide (LPS) of gram–negative bacteria (anti–LPS–sIgA) in saliva.
Results. We have identified significantly higher levels of sIg A and anti–LPS–sIgA in the saliva ofpatients with recurrent pyelonephritis with hyperoxaluria (298±104 vs 150.1±79.3 mg/1, p<0.001) and (0.353±0.16 vs 0.211±0.09, p<0.001), respectively. In the urine ofwomen of group I we havefound a statistically significant increase in the content of TNF– a 44 [16.2–130.5] vs 21 [14.2–3.45] pg/ml (p=0.04) and lysozyme 14.0[2.5– 36.5]vs 1.45[0.12–7.5]ng/ml (p=0.002). All the studied parameters (anti–LPS–sIg A in saliva and sIg A, lactofer– rin and lysozyme urine) had a direct correlation with the level of daily oxalate excretion.

Conclusions. Overproduction of indicators of mucosal immunity may be explained by the formation of intestinal dysbiosis under the influence of continuous antibiotic therapy. The imbalance of intestinal microflora, in turn, leads to the formation of hyperoxaluria and increases the production of antibodies to LPS, sIg A, lactoferrin and lysozyme.
BIOMARKERS OF KIDNEY DAMAGE IN PATIENTS WITH ARTERIAL HYPERTENSION
Authors: Kraydaschenko O.V. , Dolinnaya M.A. 
Published: June 2018

Involving of renal tubulointerstitial tissue (TIT) with fibrosis development plays an important role in the persistent renal dysfunction. This circumstance gave rise to the attempts of neutrophil gelatinase–associated lipocalin (NGAL), interleukin–18 (IL–18) use to estimate kidney TIT lesion in chronic glomerulonephritis (CGN) and essential hypertension (EH).
Aim. To study the relationship between the biomarkers and clinical, morphological parameters reflecting renal TIT damage in CGN and EH patients.
Materials and methods. We examined 44 hypertensive patients and 49 patients with CGN and hypertension. We used data of the kidney morphological study for the analysis of renal TIT lesion in CGN patients. Levels of blood and urine NGAL and IL–18 were determined using immunoassay kits.
Results. All patients had preserved renal function. In hypertensive patients microalbuminuria (MAU) was detected in 32%. Patients with CGN had average daily proteinuria 0,5 (0,1:1,2) g/day. Morphologically tubular epithelium dystrophy was observed in 98% ofpatients with CGN. Interstitial fibrosis (IF) was seen in all patients with CGN, and 45% had focal character, while 55% – diffuse. Necrotic changes in the tubular epithelium were observed in 69% of cases, thickening of tubular basement membrane – 61% of patients. Correlation analysis found an association between the level of serum NGAL and IF(r=0,35, p=0,05), tubular basement membrane thickening (r=0,42, p=0,05); urinary NGAL levels and tubular epithelium dystrophy (r=0,29, p=0,05). Serum IL–18 was correlated with tubular epithelium dystrophy (r=0,69, p=0,05); tubular epithelium necrosis (r=0,37, p=0,05), IF (r=0,31, p=0,05). Despite normal renal function and absence of MAU (in 64% of cases), the indicators of renal TIT damage in hypertensive patients are increased.
Conclusions. Serum, urine NGAL and serum IL–18 can be used as markers of renal TIT lesion in CGN and EH patients. Serum NGAL reflects most accurately IF and tubular basement membrane changes; urine NGAL – tubular epithelium dystrophy. Serum IL–18 is an indicator of tubular epithelium necrosis, IF.
EFFECT OF CRYO PLACENTA EXTRACT ON THE STRUCTURE AND FUNCTION OF KIDNEYS RATS WITH ACUTE OR CHRONIC RENAL FAILURE
Authors: Topchii I.I. , Kondakov I.K. , Kirienko O.M. 
Published: June 2018

Summary. Material and methods. On an experimental material at modelling of acute and chronic nephritic insufficiency at 75 white not purebred rats action research a crioextract ofplacenta (CEP) on morphology and functional indicators of kidneys is carried out.
Results and discussion. Introduction CEP in early terms of damage of kidneys leads to fast elimination of changes of an epithelium of the tubular device and prevents development a CKI. The effect from introduction CEP remains about 16 weeks.
Conclusions. Under the influence ofCEP in the beginning of development of acute kidney insufficiency function of kidneys remains. At the generated chronic insufficiency function of kidneys improves for short term and introduction ofCEP does not influence on development CKI.
PENTOSAN POLYSULPHATE INFLUENCEON THE COURSE OF CHRONICKIDNEYDISEASE ST'. II - IV
Authors: Dudari O. , Shifrisi M. , Driyanska V.Ye. , Krot V.F. , Loboda O.M. , Krasiuke K. , Krylova M.H. , N.G. Alexeeva , Bryzhachenko T.P. 
Published: June 2018

The aim is to determine the effect of the drug pentosan polysulphate at the rate of disease progression in patients with CKD II - IVst., to confirmation of lipid - lowering action and setting of the drug on indicators of chronic inflammation.
Materials and methods. Tests conducted in one center for 12 weeks. The study included 60patients with CKD II - IV st. Patients were divided into 2 groups: group 1 (basic) - 30patients in addition to standard therapy receiving study medication, pentosan polysulphate, group 2 - 30 patients received only standard therapy. The drug pentosan polysulphate was administered for 8 - weeks: 100 mg. a day intramuscularly or intravenously for 3 weeks, followed by oral administration of 50 mg two times a day. The survey studied the dynamics ofdaily proteinuria, GFR, lipids, indicators of tumor necrosis factor, IL - 18 and vascular endothelial growth factor (VEGF) before and after treatment (12 week observation).
Results. It was found a significant decrease in total cholesterol and atherogenic ratio after 3 months of treatment in the study group, hypolipidemic effect is more significant than in the comparison group. The group used pentosan polysulphate proteinuria reduction was more pronounced, and was 65% against 38% in the comparison group. Pentosan polysulphate in the treatment ofCKDpatients in stages II - IV contributes to significant decrease in inflammatory cytokines (IL - 18 and TNF - a) in contrast to the comparison group.
Conclusions. The use of pentosan polysulphate in the treatment of patients with CKD stages II - IV had positive effect on lipid metabolism. Purpose pentosan polysulphate patients with CKD stages II - IV contributes to a significant reduction of the daily proteinuria and eGFR improvement, reduces levels of inflammatory cytokines: IL - 18 and TNF - a.
INFLUENCE OF ACECLOFENAK ON THE LEVEL OF PROAND ANTIINFLAMMATORY CYTOKINES IN THE PATIENTS WITH RHEUMATOID ARTHRITIS AND CHRONIC KIDNEY DISEASE
Authors: Zubl O. , Roborchuks W. , Dotsyukl G. 
Published: June 2018

The purpose - to investigate the effect of aceclofenac on the level of proand anti - inflammatory cytokines in patients with rheumatoid arthritis and chronic kidney disease.
Material and Methods: The research included 88patients: 45 with rheumatoid arthritis (RA) without chronic kidney disease (CKD), 43 with RA and CKD stage I without nephrotic syndrome. In a complex therapie of patients it is included nonsteroid anti - inflammatory drug aceclofenac. Influence of aceclofenac was compared with meloxicam. It is investigated to patients the content IL - 1$, IL - 10, TNFa, TGF$1, MCP - 1 is the urine with an immunofermental method.
Results. After two weeks of therapy with aceclofenac was revealed reduction IL - 1$ levels (p<0,05) and urinary (p<0,05), TNFa levels (p<0,05) in patients with RA and RA with CKD. Level of pro - sclerous cytokin TGFf>1 of blood (p<0,05) and urine (p<0,001) at patients with RA with CKD is reduced. Decrease in the MCP - 1 level in blood (in 1,5 times) and in urine (in 2 times) at patients with RA and CKD is revealed.
Conclusion. Application of therapy with aceclofenac for complex treatment ofpatients with RA and CKD improves efficiency of treatment for these patients through improvement cytokines of blood and urine.
PREDICTION OF UNFAVORABLE COURSE OF NEPHROTIC SYNDROME IN CHILDREN: THE MULTIMARKER PARALLEL CASCADE SYSTEM
Authors: Fomina S. 
Published: June 2018

The aim of study was to investigate the possibility of using the results of different diagnostic tests in children with nephrotic syndrome (NS) asprognostic markers during disease course.
Materials and methods. The operating characteristics of values derived in dynamic renoscintigraphy and indirect renangiography with 99mTc-DTPA, static renoscintigraphy with 99mTc-phosphate, levels of urine renospecific enzymes (N-acethyl-$-D-glucosaminidase and $-galactosidase) were estimated in 480NS children depending on disease activity.
Results. Different levels of prognostic valuefor studiedparameters were confirmed depend ofNS outcome.
Conclusion. Renoscintigraphy indexes and urine renospecific enzymes activity levels should be used as markers of the progression in NS children with the glance to optimal terms of studies' conduction and range parameters. The multimarker parallel cascade Systemfor prediction of unfavorable NS course has been initiated. This System can be regarded as the start ofa new strategic goalfordetermination ofrelevant unfavorable disease courseparameters in any branch ofmedicine.
PECULIARITIES OF NEPHROPROTECTIVE EFFECT OF SULODEXIDE IN PATIENTS WITH DIABETIC NEPHROPATHY
Authors: Topchiy I. I. , Semenovykh P. S. , Galchinskaya V. Yu. , Yakimenko Yu. S. 
Published: June 2018

Introduction. Monocyte chemoattractant protein type 1 (MCP-1) and plasminogen activator inhibitor type 1 (PAI-1) play a key role in inflammation, fibrosis and progression of renal dysfunction. Sulodexide advantages in vascular pathology treatment were proved by clinical trials, however, its efficiency in diabetic nephropathy (DN) was investigated insufficiently.
The aim of the present work – to study the influence of sulodexide on clinical and laboratory parametres, MCP-1 and PAI-1 levels in blood plasma of patients with DN.
Materials and methods. 76 type 2 diabetic patients with different stages of DN were observed. 44 patients received basic therapy with anti-diabetic drugs and ACE- inhibitors, 32 patients in addition to basic therapy were treated with sulodexide in a daily dose 600 LU. Concentrations of MCP-1 and PAI-1 in blood plasma were determined using immunoassay kits.
Results. In patients with DN when compared with healthy controls MCP-1 and RAI-1 levels in blood plasma significantly increased already at initial stages of the disease. Such changes may promote a development of inflammatory and fibrotic processes in kidneys. Progression of the disease was accompanied by more essential growth of MCP-1 and RAI-1 in DN patients with expressed clinical manifestations of kidney injury. Sulodexide treatment in patients with DN resulted in essential decrease of inflammatory and fibrotic markers and had positive effect on urinary protein excretion.
Conclusions. Glicosaminoglycans, in particular, sulodexide, is effective in complex treatment of patients with DN.