bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic silencing of RNF144A expression in breast cancer cells through promoter hypermethylation and MBD4
Authors: Zhang Ye , Yang Yin‐Long , Zhang Fang‐Lin , Liao Xiao‐Hong , Shao Zhi‐Min , Li Da‐Qiang 
Published: February 2018

AbstractEmerging evidence shows that ring finger protein 144A (RNF144A), a poorly characterized member of the Ring‐between‐Ring (RBR) family of E3 ubiquitin ligases, is a potential tumor suppressor gene. However, its regulatory mechanism in breast cancer remains undefined. Here, we report that RNF144A promoter contains a putative CpG island and the methylation levels of RNF144A promoter are higher in primary breast tumors than those in normal breast tissues. Consistently, RNF144A promoter methylation levels are associated with its transcriptional silencing in breast cancer cells, and treatment with DNA methylation inhibitor 5‐Aza‐2‐deoxycytidine (AZA) reactivates RNF144A expression in cells with RNF144A promoter hypermethylation. Furthermore, genetic knockdown or pharmacological inhibition of endogenous methyl‐CpG‐binding domain 4 (MBD4) results in increased RNF144A expression. These findings suggest that RNF144A is epigenetically silenced in breast cancer cells by promoter hypermethylation and MBD4.
CGB5 expression is independently associated with poor overall survival and recurrence‐free survival in patients with advanced gastric cancer
Authors: Yang Yuxin , Shi Yonghong , Hou Yanjuan , Lu Ying , Yang Jinliang 
Published: February 2018

AbstractThe human CGB5 gene encodes chorionic gonadotropin (hCG)β 5, which is aberrantly expressed in trophoblastic neoplasm and in some non‐trophoblastic neoplasms. Fucntional studies observed that it involved tumor initiation, growth, and metastatic outgrowth. In this study, using data from the International Cancer Genome Consortium (ICGC) and the Cancer Genome Atlas (TCGA)‐stomach adenocarcinoma (STAD), we assessed the independent prognostic value of CGB5 expression in patients with primary gastric cancer (GC). Results showed that CGB5 expression was nearly not expressed in normal GC tissues. In comparison, its expression was detected in 214 of the 415 primary GC cases (51.6%) in TCGA‐STAD and was associated with poor response to primary therapy and a higher risk of recurrence and death. In early stages, CGB5 expression was not a prognostic factor in terms of OS (HR: 1.448; 95% CI: 0.811–2.588, P = 0.211) or RFS (HR: 1.659; 95% CI: 0.778–3.540, P = 0.190). However, its expression was independently associated with unfavorable OS (HR: 1.719; 95% CI: 1.115–2.651, P = 0.014) and RFS (HR: 3.602; 95% CI: 1.708–7.598, P = 0.001) in advanced stages. Using deep sequencing data from TCGA‐STAD, we found that CGB5 expression was not related to its genetic amplification or DNA methylation in GC. Based on these findings, we infer that CGB5 expression is common in GC patients and its expression might independently predict poor OS and RFS in advanced stages, but not in early stages of GC.
Adult intussusceptions induced by a terminal ileum diverticulum: a case report
Authors: Nakazawa Nobuhiro , Suzuki Hideki , Ebara Gen , Watanabe Yu , Tsukagoshi Ritsuko , Ieta Keisuke , Morohara Koji , Osawa Hidenobu , Katayama Kazuhisa , Yasuda Yasukuni , Tanaka Shigehumi , Kuwano Hiroyuki 
Published: February 2018

Key Clinical MessageWe herein report a case of adult intussusceptions induced by a terminal ileum diverticulum. Histological examination confirmed a terminal ileum diverticulum full of feces, and it was considered an infiltrated region. The clinical characteristics of previously reported adult intussusceptions are also discussed, including jejunoileal diverticulum and surgical management.
Analytical and semi‐analytical kinetics models for design and optimization of double‐resistance resin in pulp and carbon in pulp processes with both reversible and irreversible nature
Authors: Naderi Ali , Outokesh Mohammad , Ahmadian Koudakan Payam , Ghoddocynejad Davood , Saberyan Kamal 
Published: February 2018

AbstractThe current study was aimed at developing a package of “model + algorithm” for the design of resin in pulp (RIP) and carbon in pulp (CIP) processes of gold, uranium, and base metals. For this purpose, we first formulated a double‐resistance model for irreversible adsorption (accompanied by chemical reaction) in CSTRs, and modified the McKay et al. semi‐analytical model for reversible uptake in a similar system. We then devised two algorithms for the design and optimization of reversible and irreversible RIP and CIP cascades. The developed algorithms were applied on the extraction of copper, uranium, and gold. The packages are able to specify the optimum number of stages, reactor volume (V), resin flow rate (m.), and resin hold up (φs). This shows an evident advantage over the McCabe‐Thiele method, whose only result is the number of stages. The new methods have only two adjustable parameters of diffusivity (Deff) and liquid film coefficient (k), whose determination can be carried out through simple batch experiments. As an additional work, this study also presents a method for mathematical troubleshooting for the Mc Kay et al. model.
Surface Modification on Pd Nanostructures for Selective Styrene Oxidation with Molecular Oxygen
Authors: Hu Canyu , Xia Xiaoqian , Jin Jianbo , Ju Huanxin , Wu Di , Qi Zeming , Hu Shanwei , Long Ran , Zhu Junfa , Song Li , Xiong Yujie 
Published: February 2018

AbstractThe catalytic oxidation based on molecular oxygen (O2) is one of the most preferable choices for oxidation reactions toward atom‐efficient utilization of oxidants, in which heterogeneous catalysts based on palladium (Pd) nanostructures have been commonly used. However, in terms of selective styrene oxidation, it is still a great challenge to balance reactivity and selectivity by employing pure Pd catalysts. Herein, a well‐designed model for seeking a selective oxidation catalyst based on surface lattice engineering is described. In the proposed model, Au atoms are sparsely deposited on the surface of concave Pd nanostructures. As such, the activation degree of adsorbed O2 can be altered by simply tailoring the concentration of Au atoms on surface, suppressing the formation of over‐oxidized byproducts. Moreover, the Pd@Au nanostructures reported here have sufficient plasmonic cross‐sections for light harvesting, which enables light‐driven catalysis through a photothermal effect. Taken together, the Pd@Au0.08 concave nanostructures with an Au/Pd ratio of 8% achieve 87% main product selectivity with a conversion of 44% in the aerobic oxidation of styrene using molecular O2 (1 atm) without additional initiator.
Liposome Preparation for the Analysis of Lipid‐Receptor Interaction and Efferocytosis
Authors: Voss Oliver H. , Lee Ha‐Na , Tian Linjie , Krzewski Konrad , Coligan John E. 
Published: February 2018

AbstractEfficient phagocytosis of apoptotic cells (efferocytosis) is essential for immune homeostasis. Phospholipids exposed on the surface of apoptotic cells, such as phosphatidylserine, supply important “eat‐me” signals. Liposomes are lipid bilayer vesicles that can be generated from one or several types of phospholipids of interest. Thus, these vesicles offer versatility, flexibility, and, importantly, a three‐dimensional structure for studying the interaction between lipids and their receptors as well as the lipid‐receptor interaction–mediated signaling events controlling efferocytosis by cells like professional phagocytes. Here, we describe methods to prepare liposomes, perform liposome‐based lipid–receptor binding assays, use liposomes to block efferocytosis, and utilize liposome‐coated beads as apoptotic cell surrogates for phagocytosis. © 2018 by John Wiley & Sons, Inc.
Molecular Genetic Manipulation of Sarcocystis neurona
Authors: Howe Daniel K. , Yeargan Michelle , Simpson Landon , Dangoudoubiyam Sriveny 
Published: February 2018

AbstractSarcocystis neurona is a member of the important phylum Apicomplexa and the primary cause of equine protozoal myeloencephalitis (EPM). Moreover, S. neurona is the best‐studied species in the genus Sarcocystis, one of the most successful parasite taxa, as virtually all vertebrate animals may be infected by at least one species. Consequently, scientific investigation of S. neurona will aid in the control of EPM and neurologic disease in sea mammals, while also improving our understanding of a prominent branch on the apicomplexan phylogenetic tree. These protocols describe methods that expand the capabilities to study this prominent member of the Apicomplexa. © 2018 by John Wiley & Sons, Inc.
Generation of High‐Specificity Antibodies against Membrane Proteins Using DNA‐Gold Micronanoplexes for Gene Gun Immunization
Authors: Hansen Debra T. , Craciunescu Felicia M. , Fromme Petra , Johnston Stephen A. , Sykes Kathryn F. 
Published: February 2018

AbstractMembrane proteins are the molecular interface of the cell and its environs; however, studies of membrane proteins are highly technically challenging, mainly due to instability of the isolated protein. Towards the production of antibodies that recognize properly folded and stabilized forms of membrane protein antigen, we describe a DNA‐based immunization method for mice that expresses the antigen in the membranes of dendritic cells, thus allowing direct presentation to the immune system. This genetic immunization approach employs a highly efficient method of biolistic delivery based on DNA‐gold micronanoplexes, which are complexes of micron‐sized gold particles that allow dermal penetration and nanometer‐sized gold particles that provide a higher surface area for DNA binding than micron gold alone. In contrast to antibodies derived from immunizations with detergent‐solubilized protein or with protein fragments, antibodies from genetic immunization are expected to have a high capacity for binding conformational epitopes and for modulating membrane protein activity. © 2018 by John Wiley & Sons, Inc.
BODIPY‐Pyrene and Perylene Dyads as Heavy‐Atom‐Free Singlet Oxygen Sensitizers
Authors: Filatov Mikhail A. , Karuthedath Safakath , Polestshuk Pavel M. , Callaghan Susan , Flanagan Keith J. , Wiesner Thomas , Laquai Frédéric , Senge Mathias O. 
Published: February 2018

AbstractDyads combining BODIPY as an electron acceptor and pyrene or perylene as electron donor subunits were prepared and their photophysical properties were studied by steady‐state and transient spectroscopy. Depending on the structure of the subunits and the polarity of the media, the dyads show either bright fluorescence or photo‐induced electron transfer (PeT) in solution. Charge‐transfer (CT) states formed as a result of PeT and were found to yield triplet excited states of the BODIPY. In the presence of molecular oxygen, the dyads sensitize singlet oxygen (1O2) with quantum yields of up to 0.75.
Effect of Charge State of L‐Aspartic and L‐Arginine Amino Acids on Morphology of Calcium Oxalate Monohydrate Crystals
Authors: Taranets Yuliia Volodymyrivna , Pritula Igor Mikhaylovich , Bezkrovnaya Olga Nikolaevna , Mateychenko Pavel Victorovich , Sofronov Dmitriy Semyonovich , Puzan Anna Nikolaevna 
Published: February 2018

AbstractThe influence of amino acids of different nature (L‐aspartic acid (L‐asp) and L‐arginine (L‐arg)) on the morphology and size of calcium oxalate monohydrate crystals (COM), one of the main components of pathogenic organomineral aggregates in the human organism, is studied. Addition of L‐asp and L‐arg is found to inhibit the processes of COM crystal growth, thereby decreasing the crystal size by more than twice compared to pure COM crystals. The increase in the concentration of L‐asp and L‐arg amino acids in the reaction mixture to 4 × 10−3 m and higher leads to the appearance of individual COM crystal. At 20 × 10−3 m concentration of the amino acid, individual crystals of size between 3 to 5 µm are mainly formed. L‐asp and L‐arg amino acids can block the growth of the {121} face due to the formation of hydrogen bonds, and the growth of the {100} and {010} faces by electrostatic interaction.
Effects of gum chewing exercise on maximum bite force according to facial morphology
Authors: Shirai Manami , Kawai Nobuhiko , Hichijo Natsuko , Watanabe Masahiko , Mori Hiroyo , Mitsui Silvia Naomi , Yasue Akihiro , Tanaka Eiji 
Published: February 2018

AbstractDevelopment of the masticatory system is influenced by functional needs. Furthermore, masticatory exercise can improve masticatory function. The aim of this study was to evaluate the potential effect of the gum chewing exercise on the maximum bite force (MBF) in adult subjects with different facial morphologies. MBF was measured by a portable occlusal force gauge and lateral cephalogram was used for evaluation of craniofacial morphology in 19 individuals (7 males and 12 females) with a mean age of 25.4 years (SD ± 4.3). The volunteers underwent gum chewing exercise for 5 min twice a day for 4 weeks. MBF was measured before (T1) and after the 4‐week exercise (T2). The facial morphology of the subjects was classified into the brachy (n = 7), mesio (n = 7), and dolicho (n = 5) facial types. In all three groups, exercise was associated with a significant increase in MBF, though the percent increase was highest in the dolicho facial type. We conclude that gum chewing exercise can improve masticatory performance, especially in individuals with dolicho facial morphology.
Heterojunction Engineering for High Efficiency Cesium Formamidinium Double‐Cation Lead Halide Perovskite Solar Cells
Authors: Wu Yihui , Wang Peng , Wang Shubo , Wang Zenghua , Cai Bing , Zheng Xiaojia , Chen Yu , Yuan Ningyi , Ding Jianning , Zhang Wen‐Hua 
Published: February 2018

AbstractInvited for this month′s cover is the group of Wen‐Hua Zhang at the China Academy of Engineering Physics. The image shows a novel device structure for perovskite solar cells, based on which the trap‐state and carrier transfer energy barrier are significantly reduced. The Communication itself is available at 10.1002/cssc.201702221.
Clinicians' perspective on an app for patient self‐monitoring in eating disorder treatment
Authors: Lindgreen Pil , Clausen Loa , Lomborg Kirsten 
Published: February 2018

AbstractObjectiveThe Recovery Record smartphone app is a self‐monitoring tool for individuals recovering from eating disorders. Oppositely to traditional pen‐and‐paper meal diaries, the app allows for in‐app patient–clinician linkage enabling clinicians to access patient app data anytime. The aim of our study was to explore the interdisciplinary clinical perspective on Recovery Record and its impact on treatment.MethodThirty‐one clinicians from a Danish eating disorder treatment facility participated in field studies and 23 of these in interviews. Data were generated and analyzed concurrently applying the inductive methodology of Interpretive Description.ResultsWe found two overarching themes: “Access to app data between treatment sessions”, and “The patient–clinician relationship”. Sub‐themes associated with the former were “Online obligations” in relation to the added workload of continuously monitoring patient app data, and “Prepared or prejudiced” relating to advantages and disadvantages of using patient app data as preparation for treatment sessions. Sub‐themes pertaining to the latter were “Expectation discrepancy” in relation to patients' and clinicians' divergent expectations for app usage, and “Pacified patients” regarding the clinicians' experience that the app potentially compromised the patient initiative in treatment sessions.DiscussionRecovery Record induced new and affected pre‐existing treatment and work conditions for clinicians. Clinicians were preoccupied with challenges associated with the app, for example, an added work load and potential harm to the patient–clinician collaboration. Thus, prior to adopting the app, we encourage clinicians and managements to discuss the objectives, advantages and disadvantages of adopting the app, and outline specific guidelines for patient and clinician app usage.
Constructing and evaluating a continent‐wide migratory songbird network across the annual cycle
Authors: Knight Samantha M. , Bradley David W. , Clark Robert G. , Gow Elizabeth A. , Bélisle Marc , Berzins Lisha L. , Blake Tricia , Bridge Eli S. , Burke Lauren , Dawson Russell D. , Dunn Peter O. , Garant Dany , Holroyd Geoffrey L. , Hussell David J. T. , Lansdorp Olga , Laughlin Andrew J. , Leonard Marty L. , Pelletier Fanie , Shutler Dave , Siefferman Lynn , Taylor Caz M. , Trefry Helen E. , Vleck Carol M. , Vleck David , Winkler David W. , Whittingham Linda A. , Norris D. Ryan 
Published: February 2018

AbstractDetermining how migratory animals are spatially connected between breeding and non‐breeding periods is essential for predicting the effects of environmental change and for developing optimal conservation strategies. Yet, despite recent advances in tracking technology, we lack comprehensive information on the spatial structure of migratory networks across a species’ range, particularly for small‐bodied, long‐distance migratory animals. We constructed a migratory network for a songbird and used network‐based metrics to characterize the spatial structure and prioritize regions for conservation. The network was constructed using year‐round movements derived from 133 archival light‐level geolocators attached to Tree Swallows (Tachycineta bicolor) originating from 12 breeding sites across their North American breeding range. From these breeding sites, we identified 10 autumn stopover nodes (regions) in North America, 13 non‐breeding nodes located around the Gulf of Mexico, Mexico, Florida, and the Caribbean, and 136 unique edges (migratory routes) connecting nodes. We found strong migratory connectivity between breeding and autumn stopover sites and moderate migratory connectivity between the breeding and non‐breeding sites. We identified three distinct “communities” of nodes that corresponded to western, central, and eastern North American flyways. Several regions were important for maintaining network connectivity, with South Florida and Louisiana as the top ranked non‐breeding nodes and the Midwest as the top ranked stopover node. We show that migratory songbird networks can have both a high degree of mixing between seasons yet still show regionally distinct migratory flyways. Such information will be crucial for accurately predicting factors that limit and regulate migratory songbirds throughout the annual cycle. Our study highlights how network‐based metrics can be valuable for identifying overall network structure and prioritizing specific regions within a network for conserving a wide variety of migratory animals.
Local energy agencies and cities’ participation in translocal climate governance
Authors: Lee Taedong 
Published: February 2018

AbstractWhen and under what conditions do cities commit to participating in translocal climate networks? This study examines the initial driving factors behind city participation in the Covenant of Mayors in Europe. Studying cities’ adherence or non‐adherence to transnational climate governance enhances our understanding of the links between local and national institutions, socioeconomic conditions, and transnational climate activities. Event history analyses of 261 cities in 27 European countries examine associations between when cities signed the Covenant and local and national political–economic factors. My analysis suggests that the existence of local energy agencies, other translocal climate network memberships, a high level of employment and the national adoption of renewable energy are factors that facilitate cities’ participation in the Covenant. However, country‐level drivers such as years of European Union accession, climate change awareness and gross domestic product growth are not as significant as cities’ individual attributes. The results of the analysis testify to the growing significance of local institutional capacities—knowledge, relational resources and mobilization—in expediting cities’ participation in transnational climate governance.
Cognitive performance in late adolescence and long‐term risk of early heart failure in Swedish men
Authors: Lindgren Martin , Eriksson Peter , Rosengren Annika , Robertson Josefina , Schiöler Linus , Schaufelberger Maria , Åberg David , Torén Kjell , Waern Margda , Åberg Maria 
Published: February 2018

AimsHeart failure (HF) incidence appears to increase among younger individuals, raising questions of how risk factors affect the younger population. We investigated the association of cognitive performance in late adolescence with long‐term risk of early HF.Methods and resultsWe followed a cohort of Swedish men enrolled in mandatory military conscription in 1968–2005 (n = 1 225 300; mean age 18.3 years) until 2014 for HF hospitalization, using data from the Swedish National Inpatient Registry. Cognitive performance (IQ) was measured through a combination of tests, separately evaluating logical, verbal, visuospatial, and technical abilities. The results were standardized, weighted, and presented as stanines of IQ. The association between IQ and risk of HF was estimated using Cox proportional hazards models. In follow‐up, there were 7633 cases of a first HF hospitalization (mean age at diagnosis 50.1 years). We found an inverse relationship between global IQ and risk of HF hospitalization. Using the highest IQ stanine as reference, the adjusted hazard ratio for the lowest IQ with risk of HF was 3.11 (95% confidence interval 2.60–3.71), corresponding to a hazard ratio of 1.32 (95% CI 1.28–1.35) per standard deviation decrease of IQ. This association proved persistent across predefined categories of HF with respect to pre‐existing or concomitant co‐morbidities; it was less apparent among obese conscripts (P for interaction =0.0004).ConclusionIn this study of young men, IQ was strongly associated with increased risk of early HF. The medical profession needs to be aware of this finding so as to not defer diagnosis.
Getting (back) on track
Authors: Carrick Alexandra 
Published: February 2018

AbstractThe world conference (Conference of the Parties (COP23)) under the UN Framework Convention on Climate Change took place in Bonn (Germany) from 6 to 17 November. Speakers at the meeting stressed the severity of the threat from global warming and the urgent need to act but what commitments came out of the COP23 meeting? Alexandra Carrick gives a short summary of the meeting and some of the energy outcomes of the conference.
Natural occurrence and production of tenuazonic acid in wine grapes in Argentina
Authors: Prendes Luciana P. , Fontana Ariel R. , Merín María G. , D´ Amario Fernández Agustina , Bottini Rubén , Ramirez María L. , Morata de Ambrosini Vilma I. 
Published: February 2018

AbstractA survey was carried out to determine natural occurrence of tenuazonic acid (TA) in healthy and rotten wine grapes samples from different varieties (n = 37) collected during 2016 vintage in the region ofDOCSan Rafael (Argentina). In addition, inoculation experiments with threeAlternaria alternatastrains in wine grapes were done to elucidateTAproduction and its major influencing factors. The 16.2% (6/37) of total wine grape samples showedTAcontamination with 4% (1/25) of incidence in healthy samples (77 μg·kg−1) and 42% (5/12) in rotten samples (10–778 μg·kg−1). Malbec, Cabernet Sauvignon, and Syrah varieties showedTAcontamination, whereas Bonarda, Ancelota, Torrontés, Semillón, and Chenin did not. During inoculation experiments in wine grapes, two of three strains were able to produceTAamong the evaluated conditions and the highestTAproduction was observed at 15°C and 25°C after 24 days of incubation. Nutritional composition of grapes results appropriate forA. alternatainfection andTAproduction and, together with the adequate field conditions, favorsTAnatural occurrence in wine grapes.