bluuebunny/crossref_metadata_2025_split huggingface dataset:
PHARMACOLOGICAL CARDIAC PROTECTION: WHAT IS NEW?
Authors: Kozlov I. A. 
Published: May 2019

The lecture is devoted to the current state of pharmacological cardiac protection in patients with a high cardiac risk undergoing non-cardiac and cardiac surgery with cardiopulmonary bypass. It describes the main etiopathogenetic mechanisms of ischemic reperfusion myocardial damage. The possible cardioprotection mechanisms when using inhalation anesthetics, β-adrenoreceptor blocking agents, calcium channel blockers, statins, nitrates, α2 -agonists, ACE inhibitors and sartans, levosimendan, phosphocreatine, and glucose-insulin-potassium mixture are analyzed. New research data are reviewed, including recent meta-analyses and extensive randomized studies aimed to assess the efficacy of the above medicines. It presents contemporary clinical guidelines on the use of pharmacological cardioprotection in various clinical situations. It has been concluded that the range of drugs recommended for cardioprotection is constantly changing in accordance with latest data, and updating the scientific information on this issue is an important to improve the professional competence of anesthesiologists and emergency physicians.
Impact of biotic interactions on the survival of emerging pathogen Acinetobacter baumannii in aquatic media
Authors: Dekić Svjetlana , Hrenović Jasna , Herlyn Holger , Špoljar Maria , Ivanković Tomislav 
Published: May 2019

Abstract
Acinetobacter baumannii is an opportunistic pathogen causing infections in immunocompromised patients. Recent studies recorded its persistence in a variety of abiotic conditions, but data regarding the biotic interactions with other microorganisms are limited. The aim was to assess the interaction of clinically relevant A. baumannii with common faecal bacteria Escherichia coli and Enterococcus faecium. Additionally, the interaction with a bdelloid rotifer Adineta vaga as a potential agent for biological control of A. baumannii was examined. Experiments were conducted in nutrient-poor spring water (SW) and nutrient-rich diluted nutrient broth (DNB) at 22 °C. A. baumannii coexisted with E. coli and E. faecium in both media, suggesting the absence of inter-bacterial competition in long-term survival. No difference in the survival of pandrug-resistant, extensively drug-resistant or antibiotic sensitive isolates of A. baumannii was observed. Rotifers contributed to the removal of all tested bacteria, particularly in SW. Rotifers were able to remove 5.5 ± 1.3 log CFU/mL of A. baumannii in SW and 3.5 ± 1.7 log CFU/mL in DNB. Additionally, no intracellular growth of A. baumannii inside A. vaga was detected. In wastewater treatment plants and drinking water facilities, grazing by rotifers might be useful for the removal of emerging human pathogens such as A. baumannii from water.
Mental Well-being of the First and Final-Year Medical and Dental Students of Kermanshah University of Medical Sciences
Authors: Rezaei Fatemeh , Karimi Kambiz , Omidpanah Neda 
Published: May 2019

Background and Aim:Medical and dental students are predisposed to developing psychiatric disorders owing to heavy physical, intellectual, and emotional activities. This study was conducted to evaluate the mental health status of medical and dental students.Materials and Methods:This cross-sectional study was conducted on the first- and final-year medical and dental students at Kermanshah University of Medical Sciences in 2016. The mental health status was measured by the General Health Questionnaire-28 (GHQ-28) involving somatic, social, anxiety, and depression domains. Scores > 6 (out of 28) and > 2 (out of 7) were considered to show psychiatric disorder for each domain, respectively. Data were analyzed by SPSS-18 using Kruskal-Wallis and Chi-square tests (P< 0.05).Results:The participants consisted of 246 students (39.4% first- and 37.8% last-year medical students; 8.1% first- and 14.6% last-year dental students) aged 18-37 years (22.6 ± 3.3). Psychiatric disorder, somatic symptoms, social dysfunction, anxiety, and depression were experienced by 44.7, 42.7, 46.3, 49.6, and 28.5% of the students, respectively. The prevalence of psychiatric disorder was comparable between the first- and final-year medical students (40.2% and 48.4%, respectively;P= 0.258). The final-year dental students significantly underwent more psychiatric disorder than the first-year dental students (61.1% and 20%, respectively;P= 0.003). There were no significant differences between the medical and dental students in the first (P= 0.089) and final (P= 0.196) years. There was also no significant association between the occurrence of psychiatric disorder and gender and age group (P> 0.05).Conclusion:Psychiatric disorder was common among dental and medical students. Hence, preventive and control measures are suggested to improve their mental health.
Inconsistent Use of Condom in Italian HIV-Serodiscordant Heterosexual Couples as Revealed by the Detection of Y Chromosomal (Yc) DNA in Vaginal Swabs
Authors: Fiore Jose Ramon , Zoboli Fabio , Di Stefano Mariantonietta , Fasano Massimo , Alwazzeh Marwan Jabr , Faleo Pina , Elamin Mohamed Omar Elnour , Bruno Serena , Santantonio Teresa Antonia 
Published: May 2019

Introduction:
The prevention of transmission of HIV infection is based on the regular and correct use of condom and studies on transmission rates are generally based on the self-report of condom use.
However, consistent data on different population suggest that this often leads to overreporting possibly due to social desirability. In addition, self-report of condom use does not consider improper use or breakage.

Methods:
Vaginal biomarkers were proposed to detect exposure to semen and among these detection of chromosome Y DNA (Yc) appeared promising in different research settings.
Here, we searched for Yc in vaginal swabs of 33 Italian women, engaged in a regular heterosexual relationship with a HIV serodiscordant partner and reporting a regular use of condom during sexual intercourses.

Results & Discussion:
In 10 (30.3%) women Yc was detected, especially if the infected partner was male and if the couple did not have sons. This is confirmed in Italian heterosexual women and is already demonstrated in other populations: behavioural counselling is not always a valid tool and the self-reported use of condom is not fully reliable.

Conclusion:
Further studies could help in the future to individuate more effective preventive strategies for both HIV and sexually transmitted infections.
BIOGENIC POTENTIAL OF STABILIZED SILVER NANOPARTICLES USING G. SYLVESTRAE AND THEIR BIOLOGICAL ASSAYS
Authors: Anburaj Rajeswari , JOTHIPRAKASAM VINOTH 
Published: May 2019

Objective: The idea of green chemistry has gained immense fame due to replace chemical products and improves technologies to eradicate substances that are harmful to the environment. In this paper, a rapid cost-efficient method was employed using herbal extract Gymnema sylvestrae because of their biological constituents present in the sample.
Methods: Phytosynthesis of AgNPs were optimized under different reaction conditions using pH, temperature, incubated at various concentrations. Analyses of particles were revealed using UV-Vis, FTIR spectrum, morphology was observed in scanning electron microscope, particle analysis was done using Diffraction Light Scattering and bioactive constituents present in plant sample was analysed by High-performance liquid chromatography. Bioefficacy of synthesised AgNPs was assessed by means of microbicidal assay against various bacteria and fungi.
Results: UV and FTIR analysis reveals the presence of plant extract responsible for stabilization and efficient reduction. Peptides to proteins, polyphenols, and many other secondary metabolites involved in the bioreduction were identified. SEM micrograph reveals the nature, size and distribution of the sample. HPLC chromatogram indicated the presence of gymnemagenin responsible for their biological assays. Broad spectrum of microbicidal activity have been reported in 400 µl of biosynthesized AgNPs against Bacillus sp. (24.5 mm), and S. epidermis (22.3 mm).
Conclusion: Therefore G. sylvestrae synthesized silver nanoparticles were stable and acts as a reducing and capping agent detecting the presence of biomolecules. Biosynthesised AgNPs showing excellent antimicrobial activity and future prospects of this study indicates that these nanoparticles can be applied in drug delivery.
BIOGENIC SYNTHESIS OF SILVER NANOPARTICLES USING MANILKARA HEXANDRA (ROXB.) DUBARD STEM BARK EXTRACT AND IT’S PHYSICAL, CHEMICAL CHARACTERIZATION AND PHARMACEUTICAL EVALUATION
Authors: LAWRENCE A ANTONY , PRAKASH J THOMAS JOSEPH 
Published: May 2019

Objective: The present study was to synthesize nanoparticles using Manilkara hexandra stem bark extract its characterization and evaluating it by an antimicrobial and antioxidant assay.
Methods: Manilkara hexandra stem bark silver nanoparticles (MHSB-AgNPs) was done by mixing silver nitrate (1 mmol) and aqueous stem bark extract and it was analyzed by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Zeta potential, Field Emission Scanning Electron Microscopy (FE-SEM), Energy Dispersive Spectroscopy (EDAX), Thermogravimetry/Differential Thermal Analysis (TG/DTA) and Differential scanning calorimetry (DSC). The antibacterial assay was done by a well diffusion method and also examined for antifungal assay was done by disk diffusion method and antioxidant potential Diphenyl-1-picryl hydrazyl (DPPH method)
Results: Manilkara hexandra stem bark silver nanoparticles (MHSB-AgNPs) is characterized by various techniques such as UV-visible absorption spectrum ranges from 430 nm to 440 nm indicate silver nanoparticles. The Fourier Transform Infrared Spectroscopy consists of biomolecules acts as capping agent to form silver nanoparticles. Field Emission Scanning Electron Microscopy shows particle size ranges from 15 nm to 50 nm. Energy Dispersive Spectroscopy shows the presence of Silver. X-ray Diffraction corresponds to face-centered lattice planes (111), (200), (220) and (311). Dynamic Light Scattering show the range of 68 nm and Zeta potential show the negative value of-17 nm which has high stability. Silver nanoparticles is also examined by Thermogravimetry/Differential Thermal Analysis (TG/DTA) and Differential scanning calorimetry (DSC) this project the thermal stability of the nanoparticles. The aqueous stem bark is also examined by UV-visible absorption spectrum, Fourier Transform Infrared Spectroscopy (FTIR), and Gas Chromatography-Mass Spectrometry (GCMS). In GCMS 20 compounds were identified. Silver nanoparticles show high zone of inhibition in antimicrobial assays and act as a good antioxidant agent.
Conclusion: It is eco-friendly, non-toxic, and it’s easy to synthesis and it shows good result in an antimicrobial and antioxidant assay can be applied in a pharmaceutical application.
SPECTROSCOPIC AND VOLUMETRIC TECHNIQUES FOR THE ESTIMATION OF IVABRADINE IMPURITY 3,3'-(PROPANE-1,3-DIYL)BIS(7,8-DIMETHOXY-1,3,4,5-TETRAHYDRO-2H-BENZO[D]AZEPIN-2-ONE)
Authors: KUMAR S. ANIL , BHASKAR B. L. 
Published: May 2019

Objective: Two simple and sensitive techniques - one spectrophotometric and one titrimetric- have been developed for the determination of 3,3'-(propane-1,3-diyl)bis(7,8-dimethoxy-1,3,4,5-tetrahydro-2H-benzo[d]azepin-2-one) commonly known as ivabradine impurity-9 (IVA-9).
Methods: The spectrophotometric method is based on the oxidation of drug impurity by excess cerium (IV) sulphate in acidic medium and the subsequent reaction of the remaining Ce(IV) with a known amount of ferrous ammonium sulphate. The resultant ferric ion is then made to react with thiocyanate in acid medium to form a brown coloured complex which is analyzed spectrophotometrically against the reagent blank. In the volumetric method, the un-reacted Ce(IV) is titrated against standard ferrous ammonium sulphate to estimate the quantity of IVA-9.
Results: The colored complex showed an absorption maximum at 479 nm when measured spectrophotometrically. The stated methods are validated statistically using the International Council for Harmonization guidelines-ICH Q2(R1) for precision and accuracy. The method showed a linear response from 0.5 to 100µg/ml with a correlation coefficient of 0.9985
Conclusion : No estimation techniques have been reported to date for the determination of this molecule. The proposed techniques may be used for the routine quantification in its pure form and also in presence of its parent drug molecule Ivabradine.
SOLID DOSAGE FORM DEVELOPMENT OF GLIBENCLAMIDE-ASPARTAME COCRYSTAL USING THE SOLVENT EVAPORATION METHOD TO INCREASE THE SOLUBILITY OF GLIBENCLAMIDE
Authors: BUDIMAN ARIF , MEGANTARA SANDRA , APRILIANI AYU 
Published: May 2019

Objective: The solubility of a drug in water plays an important role in the absorption of the drug after oral administration. Cocrystal is one method that improves the solubility of the active pharmaceutical ingredient (API). The aim of this study was to investigate the formation of a glibenclamide (GCM)-aspartame (APM) cocrystal using the solvent evaporation method and to evaluate its solubility and dissolution rate.
Methods: Molecular docking of the GCM-APM cocrystal was observed using an in silico method. The GCM-APM cocrystal (1:2) was prepared by using the solvent evaporation method. The cocrystal of GCM-APM was evaluated by the saturated solubility test and the dissolution rate test (USP type 2 apparatus). The solvent evaporation product of GCM-APM was characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), and powder X-ray diffraction (PXRD).
Results: In silico study showed that the interaction of GCM-APM has hydrogen bonding and the potential to improve the solubility of GCM. Evaluation of the cocrystal of GCM-APM showed that the solubility and dissolution rate of the cocrystal are significantly increased. Characterization of FT-IR showed that no chemical reaction occurred in the GCM-APM cocrystal. The DSC analysis showed the changes in the melting point of GCM. Measurement of PXRD showed the formation of a new solid crystal phase that is different from GCM and APM.
Conclusion: GCM-APM has hydrogen bonding can improve the solubility and dissolution rate of GCM.
FORMULATION AND EVALUATION OF RAMIPRIL MOUTH DISSOLVING FILMS
Authors: E Jasvanth , D Teja , B Mounika , Nalluri Buchi N 
Published: May 2019

Objective: The present investigation was aimed at preparation and evaluation of mouth dissolving films (MDFs) of Ramipril to enhance patient convenience, compliance and to improve bioavailability.
Methods: MDFs with 0.5% w/w Ramipril were prepared by a solvent casting method using a wet film applicator. The effects of film formers, wetting/solubilizing, saliva stimulating agents and film modifiers on the physicomechanical and in vitro Ramipril release from MDFs were evaluated.
Results: The MDFs prepared were transparent, smooth and showed no re-crystallization upon storage. MDFs casted with hydroxypropyl methylcellulose (HPMC) E3 as film former and polyethylene glycol (PEG-400) as plasticizer showed superior Ramipril release rates and good physicomechanical properties when compared to MDFs with E5 and E15 as film formers. HPMC E3 MDFs with polyvinyl pyrrolidone K30 (PVP K30) and sodium lauryl sulphate (SLS) gave superior drug release properties than MDFs without PVP K30 and SLS. The HPMC E3 MDFs with citric acid (CA) as saliva stimulating and xylitol as soothing agent gave significantly superior in vitro drug release than the MDFs without CA and xylitol. Release kinetics data reveals diffusion as a drug release mechanism.
Conclusion: From the obtained results, it can be concluded that the administration of Ramipril as MDF may provide a quick onset of action with enhanced oral bioavailability and therapeutic efficacy.
VALIDATION AND QUANTITATIVE ANALYSIS OF CARMINE AND RHODAMINE B IN LIPSTICK FORMULATION
Authors: NEVITASARI REYNA , Rohman Abdul , MARTONO SUDIBYO 
Published: May 2019

Objective: The objective of this study was to validate reversed phase-high performance liquid chromatography (RP-HPLC) method using photo-diode array detector for the simultaneous determination of carmine (CAR) and rhodamine B (RHO B) in lipstick products.
Methods: CAR and RHO B were analysed using Cosmosil C18 column (250 mm x 4.6 mm i.d., 5 µm) using Shimadzu LC 20AD chromatograph equipped with photo-diode array (PDA) detector at 245-600 nm. The mobile phase used was acetonitrile-phosphate buffer pH 3.4 (5.5:4.5 v/v) delivered isocratically at flow rate of 1.0 ml/min, using column temperature at 35 °C. The optimized HPLC condition was validated by determining several characteristics including selectivity, linearity, sensitivity expressed by the limit of detection and limit of quantification, precision and accuracy.
Results: The optimized HPLC condition could separate CAR and RHO B successfully with resolution value>2.0. The method was linear over studied concentration with a coefficient of determination (R2) 0.9999. The relative standard deviation (RSD) values were lower than those required RSD Horwitz and the recovery values were also acceptable, therefore, the developed method was precise and accurate.
Conclusion: HPLC with PDA detector has been accurately and successfully used for quantitative analysis of CAR and RHO B. The developed method is simple and can be used for routine analysis of CAR and RHO B for quality assurance purposes.
CENTRAL COMPOSITE DESIGN APPLIED IN HPLC OPTIMIZATION FOR ANALYSIS OF TARTRAZINE AND AURAMINE O IN POWDER DRINKS
Authors: LESTARI ANNISA DYAH , Rohman Abdul , MARTONO SUDIBYO 
Published: May 2019

Objective: This study was intended to optimize reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of Tartrazine (TAR) and Auramin O (AUO) in powder drinks using experimental design of central composite design (CCD) approach.
Methods: TAR and AUO in powder drink product has same properties, therefore both analytes were analysed using C18 column (XBridge Shield RP 18 250 mm x 4.6 mm i.d., 5 µm) using Shimadzu LC 20AD chromatograph equipped with photo-diode array (PDA) detector at 300-650 nm. Some factors responsible for RP-HPLC separation of TAR and AUO including the concentration of buffer, the ratio of mobile phase and flow rate were optimized using CCD. The responses evaluated were peak area, retention time, and tailing factor. The mobile phase used was acetonitrile and ammonium acetate buffer, and acetonitrile composition was optimized at 84-86% for separation of TAR and AUO, delivered at a flow rate of 0.8–1.2 ml/min, using ammonium acetate buffer at 19-21 mmol.
Results: CCD showed that separation of TAR and AUO was influenced by flow rate, the ratio of acetonitrile and ammonium acetate concentration. These factors affected significantly to retention time, peak area, and tailing factor. The optimal condition obtained based on CCD was flow rate of 1.2 ml/min, the ratio of acetonitrile 86%, and ammonium acetate concentration of 19 mmol.
Conclusion: CCD can be used to get optimum condition for analysis of TAR and AUO in powder drink product.
Interacción de distintos factores sobre la biosíntesis de tricotecenos por cepas de fusarium
Authors: Saubois 0. Adriana , Coutaz Victor R. , Cadoche Claudia , Basílico G. Juan Carlos 
Published: May 2019

Se estudió la influencia del medio de cultivo, la cepa, el tiempo y la temperatura sobre la biosintesis de tricotecenos en cinco cepas de Fusarlum toxicogénicas. Se analizó también el efecto combinado de dichas variables. Para la evaluación del grado de toxicidad se utilizó el micrométodo biológico basado en la inhibición que ejercen los tricotecenos sobre la germinación de semillas de Lepidium satlvum. (5).Analizando el efecto de las variables por separado, se constató que existe un 99% de probabilidad (<= 0.01) de que el medio de cultivo, la cepa y la temperatura incidan en la biosíntesis de tricotecenos; en tanto que para el tiempo, y dentro del rango estudiado, la probabilidad es del 95% (<= 0.05). Encuanto a la illteracción de las variables, existe un 99% de probabilidad (<= 0.01) de que el efecto combinado de medio de cultivo-tiempo-temperatura y medio de cultivo-cepa-temperatura incidan en el grado de toxicidad.
Acción antifúngica de las asociaciones de sulfametoxazol o ketoconazol con etosuccimida, fenilbutazona, ibuprofeno, gentamicina, metamizol, yoduro de potasio y otros fármacos.
Authors: Lazo Waldo 
Published: May 2019

Se obtuvo la inhibición total del desarrollo fúngico in vitro durante 48 o más horas, al agregar al medio de cultivo los fármacos siguientes: para Acremonium potronil y A. falciforme 50 ug/ml de sulfametoxazol; para Fusarium solanii sulfametoxazol 100 ug/ml más fenilbutazona 30 ug/ml más etosuccimida 100 ug/ml o sulfametoxazol 100 ug/ml más ibuprofeno 30 ug/ml más etosuccimida 100 ug/ml; para Candlda albicans y Aspergillus niger ketoconazol 0.5 ug/ml más gentamicina 3-10 ug/ml; para A. fumlgatus sulfametoxazol100 ug/ml más yoduro de potasio 100 ug/ml más metamizol 100 ug/ml o sulfametoxazol 80-100 ug/ml más fenilbutazona 20-30 ug/ml más etosuccimida 100 ug/ml; o sulfametoxazol 80 ug/ml más clorpromacina 10 ug/ml.
La constitucionalización de la mediación
Authors: Villanueva Turnes Alejandro 
Published: May 2019

Los métodos alternativos para resolver conflictos diferentes a la vía judicial han ido ganando un mayor protagonismo con el paso de los años. Por este motivo no resulta extraño que algunos países, sobre todo en América Latina, se haya producido una constitucionalización de los mismos. Esto es lo que ha sucedido en Ecuador, Estado en el que se centrará el presente estudio.
Para ello se procederá a analizar el precepto constitucional principal relativo a la materia, haciendo alusión a la ley que se encarga de desarrollarlo, pudiendo obtener una visión de este tema en este país
Surgical management of an adrenal gland tumor that had extended into the thoracic portion of the caudal vena cava in a dog
Authors: Lipscomb Victoria J. 
Published: May 2019

Abstract

CASE DESCRIPTION
A 14-year-old neutered female Border Collie with a 3-week history of collapse during exercise was evaluated because of recumbency, tachycardia, and hypotension.

CLINICAL FINDINGS
Results of biochemical testing indicated the presence of a pheochromocytoma, and CT revealed an enlarged right adrenal gland mass that extended down the right phrenicoabdominal vein into the posthepatic thoracic portion of the caudal vena cava.

TREATMENT AND OUTCOME
A midline celiotomy and median sternotomy were performed to allow en bloc removal of the right adrenal gland tumor and its tumor thrombus extension within the caudal vena cava. Temporary occlusion of the thoracic and abdominal portions of the caudal vena cava and both renal veins and the dual inflow to the liver (Pringle maneuver) were required. The venotomy and tumor and thrombus extractions required a 25-minute period of vascular occlusion. The dog had no major postsurgical complications. Histologic findings indicated that direct adrenal tumor invasion into the caudal vena cava wall had occurred along the established route of tumor extension down the phrenicoabdominal vein.

CLINICAL RELEVANCE
For the dog of this report, an adrenal tumor thrombus that extended into the thoracic portion of the caudal vena cava was surgically managed with a combined median sternotomy and midline celiotomy approach and temporary occlusion of the hepatic artery, portal vein, and abdominal and thoracic portions of the caudal vena cava. This facilitated successful manual manipulation of the tumor and enabled venotomy of sufficient size for tumor thrombus extraction.