bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the Impact of hsa_circ_0049168 on NSCLC Proliferation and Apoptosis via the miR-370/PIN1 Regulatory Network
Authors: Chen Shuangqun , Tian Yuan , Chen Mingzhi , Wu Yanjin , Zhu Ji , Li Zixuan , Yang Jian , Liu Hongcheng , Li Huafei 
Published: December 2024

Abstract

In this study, we explored the biological role and underlying mechanisms of hsa_circ_0049168 in NSCLC. Utilizing circRNA microarray analysis and bioinformatics analyzation, we identified a significant upregulation of hsa_circ_0049168 in both NSCLC tissues and cell lines. Our results demonstrate that hsa_circ_0049168, originating from the PIN1 gene, promotes tumor cell proliferation and inhibits apoptosis. Mechanistically, hsa_circ_0049168 functions by sponging miR-370, subsequently upregulating PIN1 expression, a key regulator of cellular proliferation and survival pathways. Through in vitro and in vivo experiments, we confirmed that knockdown of hsa_circ_0049168 leads to increased apoptosis and decreased proliferation of NSCLC cells. Moreover, the effects of hsa_circ_0049168 knockdown were reversed upon inhibition of miR-370, highlighting the specificity of the hsa_circ_0049168/miR-370/PIN1 regulatory axis in NSCLC. Notably, hsa_circ_0049168 was found to modulate key downstream targets, including β-catenin and cyclin D1, which play critical roles in cell cycle regulation and tumorigenesis. The clinical relevance of hsa_circ_0049168 was further underscored by its correlation with advanced tumor characteristics such as larger tumor size, poorer differentiation, and lymph node metastasis in NSCLC patients. Taken together, our findings suggest that hsa_circ_0049168 contributes to NSCLC progression via the miR-370/PIN1 axis and could serve as a promising biomarker for diagnosis and prognosis, as well as a potential therapeutic target in NSCLC.
Rehabilitation Outcomes in Patients With Complex Regional Pain Syndrome of the Ankle-foot Complex
Authors: Vacheva Danelina , Drumev Atanas 
Published: December 2024

Abstract

Background
Complex regional pain syndrome is a debilitating condition that often involves an injury or surgery, leading to chronic pain, swelling, and functional impairment in affected limbs, particularly in the ankle-foot complex. The management is challenging, as patients may experience prolonged symptoms that impact mobility and quality of life. While rehabilitation interventions aim to improve functional outcomes, their effectiveness in restoring full ankle function and mitigating long-term effects varies widely among patients. The purpose of this study was to evaluate the effectiveness of a structured rehabilitation program for functional recovery in patients with complex regional pain syndrome of the ankle-foot complex following trauma. Seventy-two patients presented with ankle-foot trauma, 23 of whom were diagnosed with CRPS.
Methods
Patients underwent a comprehensive rehabilitation program including kinesitherapy, cryotherapy, therapeutic massage, and electrotherapy. Functional recovery was assessed via goniometry, the symmetry index via the G-WALK inertial sensor, locomotor activity tests, and activities of daily life scoring, with the data analyzed via SPSS.
Results
Statistical analysis of the 23 patients with complex regional pain syndrome revealed significant improvements over three rehabilitation courses within five months. Initial assessments revealed hypotrophy of the thigh (2.5 cm) and lower leg muscles (1.5 cm), alongside swelling in the ankle joint (3.5 cm) and foot (2 cm). By the end of the rehabilitation period, hypotrophy decreased to 1 cm in the thigh and 1.5 cm in the calf, with swelling reduced to 1.5 cm in the ankle and 1 cm in the foot. Range of motion improved significantly, with dorsiflexion increasing by 10.83º and plantarflexion by 33.33º. The symmetry index improved from 64.88–80.8%, indicating partial restoration of gait symmetry. Pain levels decreased from an average of 15 (severe) to 7.5 (moderate), indicating substantial functional gains.
Conclusion
Complex regional pain syndrome following ankle-foot trauma requires a tailored rehabilitation program to restore mobility and functionality. While complete gait normalization may be prolonged, targeted rehabilitation enables significant improvements in daily activity performance.
Evaluating the Impact of Smog Emergency Policies on Air Quality and Public Perception by Using Geospatial Technologies: A Case Study of Lahore, Pakistan
Authors: Mehmood Ahsan , Usama Raja Muhammad , Mehmood Ahmad , Rizwan Samawia 
Published: December 2024

Abstract

Air pollution, particularly smog, poses a significant global health threat, with Pakistan experiencing annual smog episodes starting in October. This study examines air quality in Lahore, frequently ranked among the world's most polluted cities, focusing on the effectiveness of government initiatives like the Smog Emergency and Health and Environmental Emergency. By analyzing changes in the Air Quality Index (AQI) and pollutant concentrations (NO2, SO2, O3, CO, PM2.5) before, during, and after implementing emergency policies, we assess their impact on air quality. Data was sourced from the AQI platform and Sentinel-5P satellite through its Tropospheric Monitoring Instrument (TROPOMI) and analyzed using Google Earth Engine and ArcGIS Pro for spatial visualization. While modest improvements were noted during the emergencies, particularly in SO2 levels, these were short-lived, with significant increases in NO2 and SO2 post-emergency. Ozone levels slightly decreased post-emergency, while CO trends varied. Additionally, a survey revealed that most residents perceived no improvement in air quality during the emergency and experienced disruptions to their daily lives and financial losses. Despite low satisfaction with government actions, many expressed conditional support for future initiatives, indicating a need for more sustainable approaches to smog control.
The improvement of natural transformation procedure and the development of genome manipulation methods for Streptococcus thermophilus B-6
Authors: Sun Kexiong , Sun Yukai , Wang Jingbo , Jiang Yueqiu , Wang Bingxin , Liu Xipeng , Li Jie , Liu Huili , Zhao Youbao , Zhang Longxian , Zhang Xinfu 
Published: December 2024

Abstract

Background Streptococcus thermophilus
is invaluable in both of dairy factory and scientific research, and different S. thermophilus strains have different advantages in industry. As a result, fast, easy, biosafe, and widely applicable genome manipulation methods for S. thermophilus are of high demands. The traditional temperature-sensitive plasmid-based homologous recombination method is neither fast nor easy, the current natural transformation method is not widely applicable, and the recently established endogenous CRISPR-Cas system-assisted method is not easy in plasmid construction.
Results
In this project, we characterized the features of the natural transformation of S. thermophilus B-6 strain, and for the first time found that the DNA transport pathway activated at natural competent stage might be bidirectional. We optimized the natural transformation protocol to upgrade the transformation ratio of S. thermophilus B-6 from ~ 10− 5 to ~ 10− 2. With the improved natural transformation procedure, we developed a fast, easy, biosafe, and plasmid-independent method for the genome manipulation of this strain. We also established a novel native CRISPR-Cas system-assisted genome manipulation pathway with a higher efficiency, which did not require any new plasmid construction. By the novel genome manipulation methods, we created different CRISPR-Cas system mutant strains and a recA overexpressing strain.
Conclusions
Our finding contributes to better understanding the features of natural competence. Our convenient and biosafe genome manipulation methods will be valuable for most of the S. thermophilus strains, and will contribute to the germplasm improvement of dairy industry. Besides, the S. thermophilus mutants we generated in this project will be useful in the future cellular metabolism investigations.
Effect of Innovative Interactive Approaches to Educating Pregnant Women About Iodine Deficiency
Authors: Mohamed Heba Alkotb , Ibrahim Asmaa Abobakr , Yousef Farida Kamel , Alkaabi Ohood , Asiri Abdulaziz 
Published: December 2024

Abstract

Pregnant women are vulnerable to iodine deficiency, which poses risks to maternal and fetal health. Preventive education on iodine intake is essential to reduce these risks. This quasi-experimental study evaluated the effectiveness of innovative interactive education techniques on pregnant women’s knowledge, attitudes, and practices (KAP) regarding iodine deficiency. A total of 132 pregnant women attending Suez Canal University Hospital clinics were divided into an intervention group, which received interactive education, and a control group, which received standard education. The intervention included multimedia presentations, group discussions, and digital tools focused on the importance of iodine. Results indicated significant improvements in knowledge, attitudes, and dietary practices related to iodine intake in the intervention group, with sustained effects at follow-up. These findings highlight the potential of interactive education in promoting preventive health behaviors among pregnant women.
Parkinson’s Disease Movement Disorder Nurse Specialist Demographic Survey 2021
Authors: Williams Susan , Tsui David , Zeppel Melanie 
Published: December 2024

Abstract
Parkinson’s disease is a common neurological condition. Patients benefit from patient centred care provided by a Parkinson’s disease or movement disorder nurse specialist. This study is the second in a series of annually recurring publications to progressively monitor the growth and development of the PDMDNS profession in Australia. Results will firstly; provide evidence to support the PDMDNS professional body in Australia to advocate for adequate resources, sufficient training and appropriate levels of qualifications and pay grades, secondly; assist in identifying gaps in the provision of services to people living with PD.
Streamflow simulation at different temporal scales under rating curve uncertainty conditions using machine learning models
Authors: Mena Nahom Bekele , Ukumo Tigistu Yisihak , Haile Ashagre Paulos Paulos , Edamo Muluneh Legesse Legesse , Olango Nardos Tesfalem 
Published: December 2024

ABSTRACT

Reducing uncertainty in streamflow simulation is vital for effective water resource management. The impact of uncertainty in model calibration data (discharge), commonly derived from the rating curve, is often overlooked. This study applies the Monte Carlo simulation technique (MCST) to assess uncertainty in the rating curve. Advanced machine learning (ML) models, bidirectional long short-term memory (BiLSTM), and bidirectional gated recurrent units (BiGRUs) were used comparatively to evaluate the propagation of this uncertainty onto streamflow simulation on both daily and monthly temporal scales. Different sets of streamflow data, derived from the fitted curve and its lower and upper uncertainty bands, were utilized to train ML models independently. The results show the substantial impact of rating curve uncertainty in streamflow simulations, with the BiGRU model surpassing the BiLSTM model on both scales. As a result, the uncertainty in the rating curve results in an uncertainty of the streamflow of up to 30 and 25% on daily and monthly simulations, respectively. These findings underscore the importance of considering rating curve uncertainty in streamflow simulation to ensure accurate and reliable results. Therefore, streamflow should be treated as an uncertain variable and managed by incorporating rating curve uncertainty in decision-making.
Multi-objective cropping pattern optimization and comparative assessment with the food-energy-water nexus
Authors: Hacısüleyman Volkan , Özger Mehmet 
Published: December 2024

ABSTRACT
Agriculture is the largest consumer of water, accounting for nearly 70% of global freshwater consumption, and it also uses about 30% of the world's energy. This creates an increasing challenge for the efficient use of water and energy resources while adequately meeting food demand. Therefore, understanding the interrelations between food, energy, and water resources is crucial. In this study, a multi-objective linear programming model was employed to identify alternative scenarios for optimal cropping areas that minimize water use in agriculture and maximize agricultural income. Different weight coefficients were assigned to these objective functions to generate various cropping scenarios. Once the optimal cropping patterns for each scenario were determined, parameters such as water use, energy requirements, agricultural revenue, and carbon dioxide emissions were calculated based on the food-energy-water nexus. The results for each alternative crop pattern scenario were then analyzed. The results indicate that prioritizing the objective of minimizing water use leads to an average reduction of 3.35% in water use, 1.18% in energy demand, and 0.26% in carbon dioxide emissions, while agricultural income increases by an average of 1% compared to the base scenario. Conversely, when maximizing agricultural income is prioritized, there is an average increase of 2.05% in agricultural income.
Sustainable Stabilization of Brown and Green Clays Using Industrial By-products
Authors: Abdel-Jaber Mu’tasim , Ibrahim Reem , Al-shamayleh Rawan , AlQatamin Abdullah , Khrisat Mohammad , Barghouthi Amjad , Tarawneh Bashar 
Published: December 2024

Background
The disposal of industrial by-products and the rising cost of construction materials are critical issues globally, particularly in developing nations such as Jordan. Brown and green clay are considered problematic types of soft clay, as they fail to meet the necessary specifications for use in construction.

Objective
This study aims to assess the efficiency of Cement Kiln Dust (CKD) and Silica Fume (S.F.) as stabilizing agents for brown and green clays. The research explores how CKD and S.F. can be used as cost-efficient and eco-friendly alternatives to enhance the mechanical properties of soft clays, improving their viability for construction.

Methods
An experimental approach was used to investigate the impact of CKD and S.F. on brown and green clay stabilization. The study examines the effect of varying CKD and S.F. concentrations on the clays' plasticity, compaction, unconfined compressive strength, and consolidation properties. CKD was tested at four different levels (0%, 5%, 10%, and 15%), as was S.F. (0%, 5%, 10%, and 15%).

Results
The findings revealed a notable enhancement in the engineering properties and behavior of both brown and green clay, with reductions observed in the plasticity index (P.I.), compression index (Cc), compressive strength (qc), and maximum dry density (γd).

Conclusion
Adding 15% CKD to brown clay resulted in the most significant improvements after a curing period of 7 days based on the unconfined compressive strength test results.
Toxicological encounters in Papua New Guinea
Authors: Richens John 
Published: December 2024

A selection of toxicological incidents encountered by the author in Papua New Guinea during the 1980s is presented. Toxins related to lifestyle include Clostridium perfringens Type B toxin, the cause of pigbel (a necrotizing enteritis of children), an unidentified toxin in spoiled sago causing acute intravascular haemolysis and accidental carbon monoxide poisoning arising in a vehicle with leaking exhaust. Two contrasting causes of paralysis are discussed: hypokalaemic periodic paralysis, induced by carbohydrate overload, and neurotoxic envenomation caused by the Papuan death adder. Self inflicted poisonings include suicide attempts with paraquat and battery acid and a mass poisoning incident caused by consumption of a methanol-isopropanol mixture at a party. Also discussed are a drug-induced oculogyric crisis in a patient developing hepatitis A and a mysterious dilated pupil contracted by the author, possibly from a trumpet flower.
Exploring the General Acceptability and User Experience of a Digital Therapeutic for Cognitive Training in a Singaporean Older Adult Population: Qualitative Study
Authors: Kwek Siong Peng , Leong Qiao Ying , Lee V Vien , Lau Ni Yin , Vijayakumar Smrithi , Ng Wei Ying , Rai Bina , Raczkowska Marlena Natalia , Asplund Christopher L , Remus Alexandria , Ho Dean 
Published: December 2024

Background
Singapore’s large aging population poses significant challenges for the health care system in managing cognitive decline, underscoring the importance of identifying and implementing effective interventions. Cognitive training delivered remotely as a digital therapeutic (DTx) may serve as a scalable and accessible approach to overcoming these challenges. While previous studies indicate the potential of cognitive training as a promising solution for managing cognitive decline, understanding the attitudes and experiences of older adults toward using such DTx platforms remains relatively unexplored.

Objective
This study aimed to characterize the general acceptability and user experience of CURATE.DTx, a multitasking-based DTx platform that challenges the cognitive domains of attention, problem-solving, and executive function in the Singaporean older adult population.

Methods
A total of 15 older adult participants (mean age 66.1, SD 3.5 years) were recruited for a 90-minute in-person session. This session included a 30-minute playtest of CURATE.DTx, followed by a 60-minute semistructured interview to understand their overall attitudes, experience, motivation, and views of the intervention. Interviews were audio-recorded and transcribed verbatim, then analyzed using an inductive approach. Thematic analysis was used to identify emerging patterns and insights.

Results
A total of 3 main themes, and their respective subthemes, emerged from the interviews: comprehension, with subthemes of instruction and task comprehension; acceptability, with subthemes of tablet usability, engagement and enjoyment, and attitude and perceived benefits; and facilitators to adoption, with subthemes of framing and aesthetics, motivation recommendations and the role of medical professionals. Our findings revealed that participants encountered some challenges with understanding certain elements of CURATE.DTx. Nevertheless, they were still highly engaged with it, finding the challenge to be enjoyable. Participants also showed a strong awareness of the importance of cognitive training and expressed a keen interest in using CURATE.DTx for this purpose, especially if recommended by medical professionals.

Conclusions
Given the positive engagement and feedback obtained from Singaporean older adults on CURATE.DTx, this study can serve as a basis for future platform iterations and strategies that should be considered during implementation. Future studies should continue implementing an iterative codesign approach to ensure the broader applicability and effectiveness of interventions tailored to this demographic.
TRANSFORMASI IKONOGRAFI TAMAN KOTA ASEMBAGUS KE DALAM BATIK DESI NURUL KOMALA SARI
Authors: Sari Desi Nurul Komala , Sudarmawan Agus , Sudita I Ketut 
Published: December 2024

Transformasi Ikonografi Taman Kota Asembagus ke dalam Batik Desi Nurul Komala Sari diteliti dengan menggunakan metode Penelitian Tindakan (Action Research) yang digolongkan penelitian kualitatif. Penelitian ini bertujuan untuk mendeskripsikan 1) Keberadaan Taman Kota Asembagus, 2) Semiotika dasar ikonik Taman Kota Asembagus pada visualisasi batik karya Desi Nurul Komala Sari. Teknik pengumpulan data yang digunakan meliputi, observasi, wawancara, dokumentasi dan kepustakaan. Hasil penelitian ini menunjukkan (1) Keberadaan Taman Kota Asembagus yaitu asal mula nama Asembagus, berdirinya Taman Kota Asembagus, ikonik dan keadaan lingkungan Taman Kota Asembagus. (2) Semiotika dasar ikonik Taman Kota Asembagus pada visualisasi batik karya Desi Nurul Komala Sari yang meliputi deskripsi semiotika dan unsur-unsur seni rupa, alat dan bahan, proses pembuatan karya batik, nilai semiotika karya batik Desi Nurul Komala Sari berdasarkan unsur-unsur seni rupa. Kesimpulan dari penelitian yang dilakukan adalah transformasi ikonografi Taman Kota Asembagus ke dalam karya batik Desi Nurul komala sari dengan tujuan mendeskripsikan keberadaan Taman Kota Asembagus dan semiotika ikonik pada visualisasi batik Desi Nurul Komala Sari. Setelah tervisualkan karya batik tersebut didiskusikan bersama dosen, pengamat seni, dan teman sejawat sebagai evaluasi karya untuk pengembangan diri peneliti dan sebagai referensi peneliti selanjutnya.

Kata Kunci: Transformasi,Taman Kota Asembagus, Batik.
WUJUD DAN FUNGSI FACE ART PADA SENI PERTUNJUKAN JARANAN BUTO DI DESA JAMBEWANGI KABUPATEN BANYUWANGI
Authors: Faizin Ahmad Nur , Ardana I Gusti Nengah Sura , Sudiarta I Wayan 
Published: December 2024

Seni pertunjukan Jaranan Buto di desa Jambewangi, Kabupaten Banyuwangi, memperlihatkan keberagaman dalam tata rias wajah yang dikenal sebagai face art. Penelitian ini bertujuan untuk menggali wujud dan fungsi dari face art dalam konteks seni pertunjukan tersebut. Penelitian ini menggunakan metode deskriptif kualitatif dengan melakukan observasi langsung terhadap pertunjukan Jaranan Buto dan wawancara dengan para seniman serta ahli budaya setempat, guna untuk mendeskripsikan 1) Bentuk face Art apa saja yang ada di kesenian jaranan buto di desa Jambewangi. 2) Makna apa saja yang terkandung dalam face Art kesenian jaranan buto di desa Jambewangi. 3) Apa fungsi face Art yang terdapat pada kesenian jaranan buto di desa Jambewangi. Data yang terkumpul dianalisis secara tematik untuk mengidentifikasi motif, warna, dan desain yang digunakan dalam face art, serta untuk mengungkap fungsi- fungsi khususnya dalam konteks budaya dan pertunjukan. Hasil penelitian menunjukkan bahwa wujud face art pada Jaranan Buto mencakup berbagai motif geometris dan simbol-simbol mistis yang menggambarkan karakter tokoh dan suasana pertunjukan. Fungsi face art dalam seni pertunjukan ini melibatkan aspek identitas karakter, ekspresi emosi, dan penciptaan suasana mistis yang kental. Selain itu, face art juga berperan dalam melestarikan warisan budaya daerah dan meningkatkan daya tarik visual pertunjukan. Penelitian ini diharapkan dapat memberikan pemahaman lebih lanjut mengenai peran face art dalam seni pertunjukan Jaranan Buto, serta memberikan kontribusi pada pelestarian dan pengembangan seni tradisional di Indonesia.

Kata Kunci: wujud, fungsi, face art, Jaranan Buto
PEMBELAJARAN SENI RUPA DALAM IMPLEMENTASI KURIKULUM MERDEKA BELAJAR (STUDI KASUS DI SDN 1 SUDAJI)
Authors: Win Allyce , Suartini Luh , Sutrisno Langen Bronto 
Published: December 2024

Penelitian ini mendokumentasikan hasil kegiatan pembelajaran Seni Rupa dengan menggunakan kurikulum Merdeka Belajar di SDN 1 Sudaji. Perencanaan pembelajaran dilakukan melalui penyusunan modul ajar oleh guru mata pelajaran terkait. Pelaksanaan pembelajaran dirancang dengan lingkungan interaktif, inspiratif, dan menyenangkan, yang membangkitkan semangat belajar, memberikan ruang bagi prakarsa, memberikan keteladanan, serta fasilitasi pada setiap pembelajaran Seni Rupa di kelas 5 SDN 1 Sudaji sesuai dengan kebutuhan siswa. Guru juga menerapkan model pembelajaran Quantum Learning, Role Playing, dan Integrated Learning. Pendekatan ini merujuk pada poin penilaian, termasuk performance assessment, formatif, dan sumatif pada setiap pertemuan pembelajaran. Evaluasi dilakukan dengan membandingkan proses pembelajaran dengan penilaian tengah semester (PTS) dan penilaian akhir semester (PAS) berdasarkan nilai kehadiran dan tugas harian siswa. Temuan dari penelitian ini dapat memberikan wawasan terhadap efektivitas kurikulum Merdeka Belajar dalam konteks pembelajaran Seni Rupa, memberikan pandangan terkait metode pengajaran yang berhasil, dan mengevaluasi dampak implementasi kurikulum tersebut terhadap peningkatan pemahaman dan partisipasi siswa di bidang Seni Rupa.

Kata kunci: Pembelajaran, Seni Rupa, Kurikulum Merdeka