bluuebunny/crossref_metadata_2025_split huggingface dataset:
Yeast Genomic Screens Identify Kinesins as Potential Targets of the Pseudomonas syringae Type III Effector, HopZ1a
Authors: Lee Amy Huei-Yi , Bastedo D. Patrick , Lo Timothy , Middleton Maggie A. , Youn Ji-Young , Kireeva Inga , Lee Jee Yeon , Sharifpoor Sara , Baryshnikova Anastasia , Zhang Jianfeng , Wang Pauline W. , Peisajovich Sergio G. , Constanzo Michael , Andrews Brenda J. , Boone Charles M. , Desveaux Darrell , Guttman David S. 
Published: July 2018

ABSTRACTGram-negative bacterial pathogens inject type III secreted effectors (T3SEs) directly into host cells to promote pathogen fitness by manipulating host cellular processes. Despite their crucial role in promoting virulence, relatively few T3SEs have well-characterized enzymatic activities or host targets. This is in part due to functional redundancy within pathogen T3SE repertoires as well as promiscuous individual T3SEs that can have multiple host targets. To overcome these challenges, we conducted heterologous genetic screens in yeast, a non-host organism, to identify T3SEs that target conserved eukaryotic processes. We screened 75 T3SEs from the plant pathogen Pseudomonas syringae and identified 16 that inhibited yeast growth on rich media and eight that inhibited growth on stress-inducing media, including the acetyltransferase HopZ1a. We focused our further analysis on HopZ1a, which interacts with plant tubulin and alters microtubule networks. We first performed a Pathogenic Genetic Array (PGA) screen of HopZ1a against ~4400 yeast carrying non-essential mutations and found 95 and 10 deletion mutants which reduced or enhanced HopZ1a toxicity, respectively. To uncover putative HopZ1a host targets, we interrogated both the genetic- and physical-interaction profiles of HopZ1a by identifying yeast genes with PGA profiles most similar (i.e. congruent) to that of HopZ1a, performing a functional enrichment analysis of these HopZ1a-congruent genes, and by analyzing previously described HopZ physical interaction datasets. Finally, we demonstrated that HopZ1a can target kinesins by acetylating the plant kinesins HINKEL and MKRP1.ARTICLE SUMMARYBacterial pathogens utilize secretion systems to directly deliver effector proteins into host cells, with the ultimate goal of promoting pathogen fitness. Despite the central role that effectors play in infection, the molecular function and host targets of most effectors remain uncharacterized. We used yeast genomics and protein interaction data to identify putative virulence targets of the effector HopZ1a from the plant pathogen Pseudomonas syringae. HopZ1a is an acetyltransferase that induces plant microtubule destruction. We showed that HopZ1a acetylated plant kinesin proteins known to regulate microtubule networks. Our study emphasizes the power of yeast functional genomic screens to characterize effector functions.
Pulses of melanopsin-directed contrast produce highly reproducible pupilresponses that are insensitive to a change in background radiance
Authors: McAdams Harrison , Igdalova Aleksandra Sasha , Spitschan Manuel , Brainard David H. , Aguirre Geoffrey K. 
Published: July 2018

AbstractPurposeTo measure the pupil response to pulses of melanopsin-directed contrast, and compare this response to those evoked by cone-directed contrast and spectrally-narrowband stimuli.Methods3-second unipolar pulses were used to elicit pupil responses in human subjects across 3 sessions. Thirty subjects were studied in Session 1, and most returned for Sessions 2 and 3. The stimuli of primary interest were “silent substitution” cone‐ and melanopsin-directed modulations. Red and blue narrowband pulses delivered using the post-illumination pupil response (PIPR) paradigm were also studied. Sessions 1 and 2 were identical, while Session 3 involved modulations around higher radiance backgrounds. The pupil responses were fit by a model whose parameters described response amplitude and temporal shape.ResultsGroup average pupil responses for all stimuli overlapped extensively across Sessions 1 and 2, indicating high reproducibility. Model fits indicate that the response to melanopsin-directed contrast is prolonged relative to that elicited by cone-directed contrast. The group average cone‐ and melanopsin-directed pupil responses from Session 3 were highly similar to those from Sessions 1 and 2, suggesting that these responses are insensitive to background radiance over the range studied. The increase in radiance enhanced persistent pupil constriction to blue light.ConclusionsThe group average pupil response to stimuli designed through silent substitution provides a reliable probe of the function of a melanopsin-mediated system in humans. As disruption of the melanopsin system may relate to clinical pathology, the reproducibility of response suggests that silent substitution pupillometry can test if melanopsin signals differ between clinical groups.
Rumors in organizational communication: a nightmare for HR managers
Authors: Sudhir Subin 
Published: July 2018

Purpose
Employees often engage in informal interpersonal communication within organizations. Such communication is essential for creating a better work environment. While there are documented advantages of such communication, often, such communication is plagued by rumors. The purpose of this paper is to highlight the menace posed by rumors, the motivations for employees to engage in rumormongering, and possible ways to manage the spread of these rumors.

Design/methodology/approach
The paper draws from published literature on rumor research to illustrate the menace of rumors within organizational contexts, motivation to engage in rumormongering, and possible management strategies.

Findings
The paper identifies the menace of rumors in organizations, illustrates the reasons why employees share rumors, and discusses the possible methods to manage the spread of rumors.

Originality/value
Rumor propagation in organizations is an extremely dynamic process. The key element in rumor management is the swiftness and agility in intervention and management of rumors using strategies described in this paper. HR managers can monitor conversations to identify potentially harmful rumors and tackle this menace.
Determinants of consumption intention of Chinese cuisines for foreigners: the mediating role of variety seeking behavior
Authors: Xuhui Wang , Muhammad Asif , Ayyub Samia 
Published: July 2018

Purpose
The purpose of this study is to explore the factors affecting the consumption intention of Chinese cuisines for the foreigners living in China. The study also explains the role of variety seeking behavior of consumers in the consumption intention of Chinese cuisines.

Design/methodology/approach
The quantitative data were obtained using a structured questionnaire that is based on Dalian, Liaoning province, of People’s Republic of China. A total of 305 responses were collected, and out of which, 282 were analyzed using structural equation modeling.

Findings
The results indicate that food novelty and utilitarian value are better predictors of consumption intention of Chinese cuisines. The food quality is also found to be significant with consumption of Chinese cuisines. The variety seeking behavior of consumers found to mediate the relationship of food quality, food novelty and utilitarian value with consumption intention toward Chinese cuisines.

Originality/value
The contribution made by the current study is twofold. First, the value of study lies in identifying the factors that are responsible for food consumption intention of foreigners in China, which is an overlooked area in the previous literature. Second, the study also establishes the role of variety seeking behavior of consumers in consumption intention. The authoritative construct of variety seeking behavior matches well with the context of the current study, as the Chinese cuisine is well renowned in the world for its wide variety. Further, the findings are valuable to the stakeholders of restaurant industry in guiding them on how best they can attract foreign consumers toward local Chinese cuisines.
Periodic oscillation of memristor-based recurrent neural networks with time-varying delays and leakage delays
Authors: Xu Changjin , Li Peiluan 
Published: July 2018

Purpose
The purpose of this paper is to investigate the existence and global exponential stability of periodic solution of memristor-based recurrent neural networks with time-varying delays and leakage delays.

Design/methodology/approach
The differential inequality theory and some novel mathematical analysis techniques are applied.

Findings
A set of sufficient conditions which guarantee the existence and global exponential stability of periodic solution of involved model is derived.

Practical implications
It plays an important role in designing the neural networks.

Originality/value
The obtained results of this paper are new and complement some previous studies. The innovation of this paper concludes two aspects: the analysis on the existence and global exponential stability of periodic solution of memristor-based recurrent neural networks with time-varying delays and leakage delays is first proposed; and it is first time to establish the sufficient criterion which ensures the existence and global exponential stability of periodic solution of memristor-based recurrent neural networks with time-varying delays and leakage delays.
Selecting Six Sigma project: a comparative study of DEA and LDA techniques
Authors: Hadi-Vencheh A. , Yousefi A. 
Published: July 2018

Purpose
Nowadays, most of the organizations have focused through the world on Six Sigma to reduce the costs, improve the productivity and enhance concerned individuals’ satisfaction, especially customers’ satisfaction. Annually, these organizations define and execute thousands of Six Sigma projects which involve a great deal of investments. But are all of these projects successful and do the organizations benefit from the above advantages? The purpose of this study is to proposing a methodology to to answer this question that: How can we reduce the risk of failure in Six Sigma projects? The first step to reduce the risk of failure in Six Sigma projects is selecting optimal ones which have the most profits and the least expected risks.

Design/methodology/approach
First, the effective criteria are recognized and defined in selecting Six Sigma projects. Then, a new data envelopment analysis (DEA) model is proposed for project selection process. A real example is resolved by the presented model. Finally, the authors use linear discriminate analysis (LDA) to examine the validity of obtained results from the proposed model.

Findings
The results show that the proposed model is a suitable tool for selecting Six Sigma Projects. The findings demonstrate that the selected projects by suggested integrated DEA model are those confirmed by LDA.

Originality/value
The paper, using a real case study, provides a mathematical model to enhance decision quality in Six Sigma project selection. Applying the specific DEA model is remarkable itself, which joined to a pioneering procedure to use LDA to validity evaluation of the results.
Adoption of homesharing platforms: a cross-cultural study
Authors: Muñoz-Leiva Francisco , Mayo-Muñoz Xavier , De la Hoz-Correa Andrea 
Published: July 2018

Purpose
The purpose of this paper is to analyze the factors that affect consumer adoption of new ways of sharing accommodation services. Moreover, the authors study the influence of culture, more specifically the uncertainty avoidance (UA) dimension, on relationships between variables in a proposed adoption model.

Design/methodology/approach
The structural equation analysis method is used to verify the proposed model. The data were collected from a panel of Spanish and Vietnamese internet users. The survey yielded a total of 418 responses for the data analysis.

Findings
The findings indicate that subjective norms and ease of use exert an effect on perceived usefulness and that intention to use affects actual use in all the groups analyzed. Furthermore, UA has a moderating effect on the adoption of homesharing platforms (HSPs).

Research limitations/implications
A larger sample and a random sampling would facilitate a more accurate generalization of the results obtained for each country. The practical implications identified in this research, along with its limitations and future research opportunities, are interesting both for scholars, service providers and designers of HSPs.

Originality/value
This study bridges a gap in the current research by increasing understanding of the role of the cultural dimensions in a technological innovation adoption model for an HSP. It also takes into account the effect of perceived risk, a dimension that has not been included in previous studies.
Anticipation of organizational change
Authors: Krogh Simon 
Published: July 2018

PurposeExisting research on the organizational implications of the introduction of new information technology (IT) has neglected to focus on the anticipation of organizational change. In this paper, the author examines the extended pre-implementation phase prior to the introduction of the largest-ever health IT (HIT) implementation in Denmark. The purpose of this paper is to expand the conceptualization of organizational change to include the neglected pre-implementation phase preceding large-scale organizational change projects.Design/methodology/approachThe research is based on qualitative data consisting of interviews, documents and observations gathered during a three-year research project in the Danish health sector. An important source of methodical inspiration has been grounded theory, which has allowed the pertinent interview themes to evolve and allowed for the gradual development of a theoretical framework.FindingsThe main finding of this paper is that the anticipatory pre-implementation phase is not simply passive waiting time for organizational members. Evidence from a three-year research project demonstrates how organizational members engage in recurring patterns of sensemaking, positioning and scripting of possible futures in preparation for the organizational changes that next generation HIT imposes. The study argues that resistance to organizational change may be better understood as resistance to having to give up institutionalized rights and responsibilities.Originality/valueThe paper offers a conceptual model—the anticipation cycle—that enables the systematic analysis of the relational mechanisms at work when organizational members are preparing for pending organizational change. Early analysis based on the anticipation cycle enables organizations and scholars to bring previously black-boxed anticipatory patterns into the equation of organizational change.
Applying an organisational agility maturity model
Authors: Gunsberg David , Callow Bruce , Ryan Brett , Suthers Jolyon , Baker Penny Anne , Richardson Joanna 
Published: July 2018

PurposeThe purpose of this paper is to identify the baseline model required to measure whole-of-organisation agility within a university information services division. The paper seeks to analyse the process of identifying and applying such a model.Design/methodology/approachThe qualitative methodology applied is that of a single case study. The organisation analysed was an Australian university’s information services division. A structured survey, based on Wendler (2014), was administered to all staff as part of a multi-phased approach, thus facilitating a triangulation process.FindingsThe current research has confirmed the applicability of Wendler’s model to the higher education information technology sector. Application of the model establishes not only a baseline agility maturity score across the whole-of-organisation but also provides granular scores based on organisational units. Triangulation of survey results is recommended to achieve a more in-depth perspective.Research limitations/implicationsFurther research comparing similarly and differently sized universities could provide valuable insights. More research is needed to extend the applicability of Wendler’s model to a wider range of domains and industries.Practical implicationsThe grouping of survey questions under particular broad themes reflected the strategic focus of the division being surveyed. Organisations implementing the proposed model will need to select themes that correspond with their respective strategic goals and culture.Originality/valueThe paper has extended the research and resultant model developed by Wendler by applying them not only to both managers and staff but also to a different domain, specifically higher education.
SHRM and context: why firms want to be as different as legitimately possible
Authors: Farndale Elaine , Paauwe Jaap 
Published: July 2018

PurposeThe purpose of this paper is to raise awareness that despite many calls for attention to a firm’s context in considering consequences for human resource management (HRM) and performance, research progress to date has been limited at best, although promising signs of change are emerging. Moreover, what has been defined as “performance” is coming under increasing scrutiny, with a more holistic concept emerging that balances both a firm’s financial performance and employee well-being. The question remains whether this is a mutual gains or conflicting outcomes situation for the firmvis-á-visthe employee.Design/methodology/approachThis paper presents a framework that facilitates a broader context-centric analysis of the HRM and performance relationship. In so doing, the authors posit that context should no longer merely be an obligatory control variable in a research design, but instead should be explicitly incorporated in both theory development and empirical model testing.FindingsThe Contextual SHRM Framework demonstrates how key organizational actors can balance competitive, heritage and institutional mechanisms to create an appropriate strategic HRM (SHRM) system capable of delivering organizational outcomes that balance financial and employee well-being outcomes, which in the long run impact societal well-being that, in turn, recreates the firm’s operating context. At the heart of the framework is an iterative process between context and the SHRM system, achieving an appropriate level of dynamic fit across the various components.Practical implicationsIn addition to empirical research, the framework has to date been widely used in executive development training, serving as a force field analysis tool allowing simultaneous consideration of the external and internal elements of a firm’s context, key organizational actors and SHRM system outcomes. HR professionals applying the framework to their organization can add value by demonstrating the clear linkage between the business strategy, the HRM system and the firm’s operating context.Originality/valueThis paper is designed to encourage new directions in future research and practice. The Contextual SHRM Framework is presented as a novel tool to facilitate advancement of the HRM and performance field of study.
Gold for now and the golden years
Authors: Lewellyn Krista 
Published: July 2018

PurposeThe purpose of this paper is to draw from regulatory focus theory, to examine the effects of the “gain/no gain” nature of stock options and retirement pay on the decision to engage in cross-border acquisitions. The moderating effects of managerial discretion arising from the external industry context and internal organizational leadership structure are also examined.Design/methodology/approachThe authors employ random effects negative binomial regression analysis with a longitudinal (2006–2016) data set of US public companies operating in four industries with differing levels of industry discretion: the oil and gas, paper and packaging, aerospace and defense, and telecommunications.FindingsThe findings indicate that both CEO in-the-money stock options and retirement pay are positively related to cross-border acquisition activity. The results also demonstrate that managerial discretion, arising from the firm’s external industry context, accentuates the positive relationship between both the value of CEO in-the-money stock options and retirement pay with cross-border acquisition activity.Practical implicationsThe findings provide implication for practice as understanding how retirement pay and stock options, both of which make up a substantial portion of overall CEO pay in the USA, motivate cross-border acquisition activity, may improve decisions by executives. The evidence also provides guidance to boards of directors who are charged with the responsibility of creating CEO compensation contracts.Originality/valueThe paper fills important gaps in the existing research on the influence of compensation elements on firm outcomes, by offering a novel explanation for how in-the-money stock options and retirement pay affect CEOs’ motivations to engage in cross-border acquisitions.
Service ecosystems: insights from a wedding
Authors: Damacena Claudio , Schmidt Serje , Gauze Ioná Plein Bolzan 
Published: July 2018

Purpose
This study aims to analyze the context of experience within a service ecosystem to understand how the different actors at a specific event, namely, a wedding, interact and contribute to co-create value.

Design/methodology/approach
A case study was used as a research strategy. Content analysis was used to analyze the data with the aid of NVIVO software.

Findings
The results indicate the importance of interaction between different actors for co-creation to occur as it does not occur in isolation and is generated for and with all the audiences of an event. In addition, during the exchange encounter, the presence of guests and the network of suppliers influenced social interaction and the perception of value.

Originality/value
Studies indicate a need for empirical research based on different contexts of service ecosystems. This study addresses the co-creation of events in an area different from those investigated to date: a wedding. In this rite of passage, the experience is highly involving and interactive, contains different audiences and potential value generation for all those involved.
l‐Rhamnosylation of wall teichoic acids promotes efficient surface association of Listeria monocytogenes virulence factors InlB and Ami through interaction with GW domains
Authors: Carvalho Filipe , Sousa Sandra , Cabanes Didier 
Published: July 2018

SummaryWall teichoic acids (WTAs) are important surface glycopolymers involved in various physiological processes occurring in the Gram‐positive cell envelope. We previously showed that the decoration of Listeria monocytogenes (Lm) WTAs with l‐rhamnose conferred resistance against antimicrobial peptides. Here, we show that WTA l‐rhamnosylation also contributes to physiological levels of autolysis in Lm through a mechanism that requires efficient association of Ami, a virulence‐promoting autolysin belonging to the GW protein family, to the bacterial cell surface. Importantly, WTA l‐rhamnosylation also controls the surface association of another GW protein, the invasin internalin B (InlB), that promotes Lm invasion of host cells. Whereas WTA N‐acetylglucosaminylation is not a prerequisite for GW protein surface association, lipoteichoic acids appear to also play a role in the surface anchoring of InlB. Strikingly, while the GW domains of Ami, InlB and Auto (another autolysin contributing to cell invasion and virulence) are sufficient to mediate surface association, this is not the case for the GW domains of the remaining six uncharacterized Lm GW proteins. Overall, we reveal WTA l‐rhamnosylation as a bacterial surface modification mechanism that contributes to Lm physiology and pathogenesis by controlling the surface association of GW proteins involved in autolysis and infection.