bluuebunny/crossref_metadata_2025_split huggingface dataset:
Periplaneta americana L. extract exerts neuroprotective effects by inhibiting endoplasmic reticulum stress via AKT-dependent pathway in experimental models of Parkinson’s disease
Authors: Cao Ting , Wang Xue-lian , Rao Jiang-yan , Zhu Hui-feng , Qi Hong-yi , Tian Zhen 
Published: November 2024

Abstract
Background
Parkinson’s disease (PD) is a chronic neurodegenerative disorder that currently has no curable strategies. More and more evidence suggests that endoplasmic reticulum (ER) stress plays an essential role in PD pathogenesis. Periplaneta americana L. (P. americana) is a traditional Chinese medicine with diverse therapeutic properties. This study aims to investigate the neuroprotective effect and underlying mechanism of P. americana in in vitro and in vivo PD models.

Methods
The exposure of SH-SY5Y cells to 1-methyl-4-phenyl-pyridinium (MPP+) was used as the in vitro PD model. MTT assay, Hoechst staining, Calcein AM-PI staining and flow cytometry were performed to measure the cell viability and apoptosis. DCFH-DA and JC-1 assay were used to measure the intracellular ROS and mitochondrial membrane potential (Δψm), respectively. Western-blot and immunostaining were conducted to detect the expression of key molecules related with ER stress. For the in vivo PD model induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydro-pyridine (MPTP), the motor function of mice was assessed by behavioral tests, the level of TH was examined by western-blot and immunostaining, the expression of key molecules related with ER stress was measured by western-blot.

Results
Periplaneta americana ethanol extract (PAE) concentration-dependently inhibited MPP+-induced cell loss and increased cell viability. PAE also remarkably attenuated ROS accumulation, the decline of Δψm as well as the excessive ER stress. The neuroprotective effects of PAE could be blocked by ROS inducer trimethylamine N-Oxide or ER stress activator tunicaymycin, while the antioxidant N-Acetyl-L-cysteine or ER stress inhibitor sodium 4-phenylbutyrate mimicked the effects of PAE. Furthermore, we found that PAE could activate AKT/GSK3β/β-catenin pathway. The effect of PAE on ROS production, Δψm and ER stress was blocked by AKT inhibitor MK-2206. In in vivo model, PAE significantly improved motor function, prevented dopaminergic neuronal loss and attenuated ER stress in substantia nigra and striatum of MPTP-treated mice. Similarly, the effects of PAE on MPTP-treated mice were also abolished by MK-2206.

Conclusions
Our results suggest that P. americana exerts neuroprotective effects through inhibiting ER stress via AKT-dependent pathway. Periplaneta americana may represent a promising therapeutic agent for PD treatment and is worthy of further being exploited.
Characteristics and therapeutic outcomes of subcutaneous panniculitis-like T-cell lymphoma with and without germline HAVCR2 mutations in Thai children and adolescents
Authors: Youthong Pimpitcha , Pakakasama Samart , Komvilaisak Patcharee , Rujkijyanont Piya , Choed-Amphai Chane , Phuakpet Kamon , Moonla Chatphatai , Polprasert Chantana , Sosothikul Darintr 
Published: November 2024

Abstract
Background
Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) is a rare subtype of non-Hodgkin lymphoma associated with hemophagocytic lymphohistiocytosis (HLH)/HLH-like systemic illnesses and germline HAVCR2 mutations. Although previous studies suggested successful treatment of SPTCL with immunosuppressive therapy (IST) without chemotherapy, IST data in pediatric SPTCL remain scarce. To explore characteristics and therapeutic outcomes, comparing IST-based and chemotherapy-based regimens in pediatric SPTCL, in this retrospective cohort study, patients with SPTCL diagnosed at age ≤20 years during 2007-2023 were enrolled from 6 hematology/oncology centers in Thailand. HAVCR2 exon 2 sequencing was performed using DNA extracted from peripheral blood or bone marrow. Presence of HLH/HLH-like systemic illnesses, treatment outcomes, and adverse events (AEs) were reviewed and analyzed.

Results
Of 22 patients with SPTCL (median age at diagnosis, 11.5 years [range, 6.0-19.0]; 63.6% males), 86.4% harbored germline HAVCR2 mutation, either homozygous (77.3%) or heterozygous (9.1%) p.Y82C variant, while 68.2% developed HLH/HLH-like systemic illnesses. Overall, 36.4% received IST as first-line treatment. Durable complete remission (CR) was achieved in 71.4% and 50.0% after first-line chemotherapy and IST, respectively (P=0.45); however, chemotherapy tended to increase any AEs compared to IST (57.1% vs. 12.5%; P=0.07). The most common AEs were hypertension (27.3%), febrile neutropenia (18.2%), and fungal infection (13.6%). Among the relapsed cases, 71.4% could reach CR after subsequent-line therapy. Fatality (4.5%) only occurred in the chemotherapy group.

Conclusions
Pediatric SPTCL in Thailand frequently involves germline HAVCR2 mutations and/or HLH/HLH-like systemic illnesses. With comparable response and modest therapy-related toxicity, IST-based regimens may alternatively be considered as first-line treatment for pediatric SPTCL.
Phase angle and body composition in long-term type 1 diabetes in adults: a comparative study in a Brazilian public reference outpatient clinic
Authors: Fenner-Pena Natália , Fajardo Virgínia Capistrano , Froes Lívia , Carvalho Paulo Augusto Miranda , Comim Fábio Vasconcellos , Sahade Viviane , Lauria Márcio Weissheimer , Torres Henrique Oswaldo da Gama 
Published: November 2024

Abstract
Introduction
Type 1 Diabetes Mellitus (DM1) affects a small percentage of the population. Nevertheless, its prevalence is currently growing with alarming data on uncontrolled cases. The importance of body composition and Phase Angle (PA), assessed by Bioelectrical Impedance (BIA), in long- term DM1 patients lies in the fact that alterations in cellular integrity and body compartments may affect risk profiles and metabolic control. The objective of this study was to compare PA and body composition parameters between adults with DM1 and healthy controls.

Methods
A comparative study was carried out in a public university outpatient clinic including a cohort of adult patients of both sexes diagnosed with DM1 and healthy controls matched by age and sex in a 2:1 ratio. Anthropometric measurements included weight, height and BMI. Using the raw BIA data of Resistance and Reactance, fat-free mass (FFM), fat mass (FM), fat-free mass index (FFMI), fat mass index (FMI), PA and standardized PA (SPA) were calculated. Means or medians were compared between the groups. Regression models were used to identify distinguishing characteristics of the groups and associations within the DM1 group (i.e. glycated hemoglobin (HbA1c), disease duration, presence of microvascular complications, capillary blood glucose, BMI and FMI).

Results
88 patients with DM1and 46 healthy controls were evaluated. PA (6.05 vs. 6.85, p = 0.000) and SPA (-1.47 vs. -0,37, p = 0.000) were lower in patients with DM1 compared to healthy controls. People with DM1 displayed higher adiposity (%FM = 29.6 vs. 27.6, p = 0.016; FMI = 7.00 vs. 6.33, p = 0.016) and lower %FFM compared to healthy controls. Most of the differences were maintained after sex stratification; however, men with DM1 showed a lower FFMI than male controls (18.2 vs. 20.16, p = 0.029).

Conclusion
Patients with DM1 present lower PA than healthy controls, which may be related to worse cell membrane integrity. Significant body composition differences between the groups and between sexes were identified, with data showing greater adiposity in women with DM1 and men displaying lower muscle mass. These findings suggest the importance of including PA and body composition evaluations in the follow-up of patients with DM1. The ultimate goal is to obtain a better metabolic control and, consequently, a better prognosis.
Phenotypic and transcriptomic profiling of induced pluripotent stem cell (iPSC)-derived NK cells and their cytotoxicity against cancers
Authors: Thongsin Nontaphat , Suwanpitak Siriwal , Augsornworawat Punn , Srisantitham Jakkrapatra , Saiprayong Kritayaporn , Jenjaroenpun Piroon , Wattanapanitch Methichit 
Published: November 2024

Abstract
Background
Adoptive immunotherapy using natural killer (NK) cells has attracted considerable interest in numerous clinical trials targeting both hematological and solid tumors. Traditionally, NK cells are primarily derived from either peripheral blood (PB) or umbilical cord blood (UCB). However, these methods can lead to variability and heterogeneity within the NK cell population. In contrast, induced pluripotent stem cell (iPSC)-derived NK (iNK) cells provide a more controlled and uniform cellular population, suitable for large-scale clinical applications. This makes iNK cells a promising option for developing “off-the-shelf” immunotherapeutic products. Nevertheless, current NK cell differentiation protocols, which rely on embryoid body (EB) cultures, are labor-intensive and susceptible to unwanted heterogeneity during differentiation. Here, we developed a more efficient approach for generating iNK cells by employing a monolayer and feeder-free differentiation protocol, alongside optimized culture media.

Methods
The iNK cells were generated using a two-step in vitro monolayer feeder-free system following NK cell development. To evaluate their maturity, phenotypic analysis was performed using flow cytometry, comparing with PB-NK cells and the NK-92 cell line. Additionally, single-cell RNA sequencing was performed to examine their transcriptomic profiles. The cytotoxic activity of the iNK cells was evaluated by co-culturing with cholangiocarcinoma (CCA) and breast cancer (BCA) cell lines in both monolayer (2D) and tumor spheroid (3D) co-culture systems.

Results
We successfully differentiated iPSCs into mesoderm (ME), hematopoietic stem/progenitor cells (HSPCs), and NK cells. The resulting iNK cells exhibited typical NK cell markers such as CD45, CD56, and CD16, and expressed key functional proteins, including both activating and inhibitory receptors. Single-cell RNA sequencing confirmed that the transcriptomic profile of our iNK cells closely resembles that of PB-NK cells. Importantly, our iNK cells demonstrated strong cytotoxic abilities against various CCA and BCA cell lines, surpassing the NK-92 cell line in both monolayer cultures and tumor spheroid cultures.

Conclusion
This study highlights the potential of iPSCs as an effective alternative cell source for generating NK cells. Using a two-step in vitro monolayer feeder-free system, we successfully generated iNK cells that not only expressed key NK cell markers and their receptors but also displayed a transcriptomic profile closely resembling PB-NK cells. Furthermore, iNK cells exhibited cytotoxicity against CCA and BCA cell lines comparable to that of PB-NK cells. This approach could pave the way for off-the-shelf NK cell products, potentially enhancing the effectiveness of adoptive NK cell therapy.
Machine learning-based methods for piecewise digital predistortion in mmW 5G NR systems
Authors: Bulusu S. S. Krishna Chaitanya , Tervo Nuutti , Susarla Praneeth , Silvén Olli , Sillanpää Mikko. J. , Leinonen Marko E. , Juntti Markku , Pärssinen Aarno 
Published: November 2024

AbstractPiecewise linearization techniques require dividing the signal into multiple pieces each linearized individually. Machine learning (ML) is one of the useful tools to perform the automatic division of these pieces. Complexity reduction in the classification of piecewise digital predistortion is possible through carefully constructing features from both the signal statistics and the power amplifier (PA) characteristics. Our paper introduces two low-complex classical ML-based methods that facilitate the classification of baseband input data into distinct segments. These methods effectively linearize PA behavior by employing tailored Volterra models corresponding to each segment. Moreover, we perform an in-depth analysis of the proposed schemes to further optimize their classification and regression complexities. The two proposed low-complexity approaches are validated by laboratory experiments and show up to 4 dB error vector magnitude (EVM) improvement over the conventional approach for a class A PA at 28 GHz. Similarly, the EVM improvement is up to 2 dB over the vector-switched general memory polynomial scheme. With only one indirect learning architecture iteration, the two proposed schemes obey the 5G new radio standard up to 6.5 dB and 7 dB output backoff, respectively.
Complete inhibition of liver acetyl-CoA carboxylase activity is required to exacerbate liver tumorigenesis in mice treated with diethylnitrosamine
Authors: Shrestha Riya , Vancuylenburg Calum S. , Beretta Martina , Zhou Mingyan , Shah Divya P. , Olzomer Ellen M. , Richards Sian L. , Hoehn Kyle L. , Byrne Frances L. 
Published: November 2024

Abstract
Background
The metabolic pathway of de novo lipogenesis (DNL) is upregulated in fatty liver disease and liver cancer. Inhibitors of DNL are in development for the treatment of these disorders; however, our previous study showed that blocking DNL unexpectedly exacerbated liver tumorigenesis when liver acetyl-CoA carboxylase (ACC) 1 and 2 enzymes were deleted in mice treated with diethylnitrosamine (DEN) and fed high fat diet. Herein, we used 3 new approaches including ACC1 vs. ACC2 isotype-selective inhibition, delaying ACC inhibition until after carcinogen treatment, and feeding mice normal chow diet to better understand the impact of ACC inhibition on liver tumorigenesis.

Methods
Six genotypes of female C57BL/6J mice with floxed ACC1 and/or ACC2 alleles were injected with DEN at 2 weeks of age followed by liver-specific knockout of ACC genes at 9 weeks. Mice were fed a normal chow diet and evaluated at 52 weeks for liver tumours.

Results
Compared to the wildtype control group, no genotype decreased tumour multiplicity or burden; however, mice completely lacking liver ACC1 and ACC2 had > 5-fold increases in liver tumour multiplicity and burden.

Conclusion
ACC inhibition exacerbated DEN-induced liver tumorigenesis only when both ACC isotypes were completely inhibited. The pro-tumour phenotype of ACC inhibition was strongly reproducible irrespective of chow or high fat feeding, and irrespective of ACC inhibition prior to or after DEN treatment. Retaining partial ACC activity at either isotype prevented tumour exacerbation in mice at risk for developing liver tumours.
A consistent diffuse-interface model for two-phase flow problems with rapid evaporation
Authors: Schreter-Fleischhacker Magdalena , Munch Peter , Much Nils , Kronbichler Martin , Wall Wolfgang A. , Meier Christoph 
Published: November 2024

AbstractWe present accurate and mathematically consistent formulations of a diffuse-interface model for two-phase flow problems involving rapid evaporation. The model addresses challenges including discontinuities in the density field by several orders of magnitude, leading to high velocity and pressure jumps across the liquid–vapor interface, along with dynamically changing interface topologies. To this end, we integrate an incompressible Navier–Stokes solver combined with a conservative level-set formulation and a regularized, i.e., diffuse, representation of discontinuities into a matrix-free adaptive finite element framework. The achievements are three-fold: First, we propose mathematically consistent definitions for the level-set transport velocity in the diffuse interface region by extrapolating the velocity from the liquid or gas phase. They exhibit superior prediction accuracy for the evaporated mass and the resulting interface dynamics compared to a local velocity evaluation, especially for strongly curved interfaces.Second, we show that accurate prediction of the evaporation-induced pressure jump requires a consistent, namely a reciprocal, density interpolation across the interface, which satisfies local mass conservation. Third, the combination of diffuse interface models for evaporation with standard Stokes-type constitutive relations for viscous flows leads to significant pressure artifacts in the diffuse interface region. To mitigate these, we propose to introduce a correction term for such constitutive model types. Through selected analytical and numerical examples, the aforementioned properties are validated. The presented model promises new insights in simulation-based prediction of melt–vapor interactions in thermal multiphase flows such as in laser-based powder bed fusion of metals.
Effectiveness of Kabat Technique versus Mirror Book Therapy on clinical and electrophysiological parameters in patients with Bell’s palsy: a comparative interventional study
Authors: Patel Priyanshi K. , Bachkaniwala Aparna A. 
Published: November 2024

Abstract
Background
Bell’s palsy significantly impacts facial function, aesthetics, and quality of life.

Objective
To compare the effects of the Kabat Technique (KT) and Mirror Book Therapy (MBT) on clinical and electrophysiological parameters in patients with Bell’s palsy.

Methods
This randomized controlled trial included 31 patients divided into two groups: KT (n = 16) and MBT (n = 15). Both groups received their respective interventions (6 days/week for 3 weeks) in addition to conventional physiotherapy. Sunnybrook Facial Grading Score and Nerve Conduction Test (amplitude and latency of frontalis, nasalis, and mentalis muscles) were assessed pre- and post-intervention.

Results
Statistical analysis (SPSS v20) revealed significant intragroup improvements in all outcome measures (p < 0.05). Intergroup comparison showed MBT had superior improvements in amplitude of frontalis and reduced latency of nasalis. Both groups demonstrated significant improvement in the Sunnybrook grade shift.

Conclusion
This study suggests that KT and MBT, combined with conventional physiotherapy, positively impact clinical and electrophysiological parameters in Bell's Palsy patients. MBT showed superior results in select Nerve Conduction Test parameters.
Broad-angle coherent perfect absorption-lasing and collimation in two-dimensional non-Hermitian phononic crystals
Authors: Kong Lingzhe , Eldegail Marwa Hamid , Xu Changqing 
Published: November 2024

Abstract
Coherent perfect absorption-lasing and collimation have been extensively studied primarily for normal incidence, leaving broad-angle scenarios relatively unexplored. In this work, we investigate a two-dimensional non-Hermitian phononic crystal, aiming to achieve broad-angle coherent perfect absorption-lasing and collimation. Our approach involves orchestrating a parity-time phase transition in the band structure through a combination of non-symmorphic glide symmetry in the lattice, gain-loss modulation, and engineering of the band structure. In both the real and imaginary parts of the band structure, exceptional points form a slab-like contour with nearly zero dispersion along the boundary of the Brillouin zone. As a result, the range of incidence angles for coherent perfect absorption-lasing and collimation is significantly expanded at the frequency of the exceptional points.
Enhanced Outcomes in Type 2 Diabetes Patients With Acute Kidney Disease Through Thiazolidinedione
Authors: Chang Li-Yang , Liao Hung-Wei , Chen Jui-Yi , Wu Vin-Cent 
Published: November 2024

Abstract

Context
Patients with diabetes are prone to acute kidney injury with the potential transition to chronic kidney disease. Few studies have investigated the role of thiazolidinedione (TZD) in these patients under acute kidney disease (AKD) phase.

Objective
We sought to examine whether using TZD during AKD could reduce the risk of future major adverse outcomes.

Design and Methods
We employed the TriNetX platform before September 30, 2022, for TZD administration to patients with type 2 diabetes mellitus (T2DM) within 90 days of an AKD diagnosis. Clinical endpoints include the risk of all-cause mortality, major adverse cardiovascular events (MACE), and major adverse kidney events (MAKE). Hazard ratios (HRs) and 95% confidence intervals were calculated with 1:1 ratio propensity score matching (PSM).

Results
Among the cohort of 263,101 patients with AKD and T2DM, we identified 2723 individuals (1.03%) who were TZD users during the AKD period. After PSM, the final cohort of TZD users included 2555 individuals, with 53.82% being male and a mean age of 64.0 ± 13.5 years. Over a median follow-up period of 1.5 years, the TZD group exhibited a lower risk across various outcomes, with HRs of 0.68 [95% confidence interval (CI), 0.57-0.81] for all-cause mortality, 0.68 (95% CI, 0.58-0.80) for MACE, and 0.75 (95% CI, 0.66-0.86) for MAKE.

Conclusion
TZD demonstrated a notable reduction in mortality, cardiovascular events, and kidney-related adverse events among T2DM patients with AKD. These findings suggest a potential benefit of TZD usage for managing cardiovascular events in T2DM patients with AKD.
Solving the Price-Setting Newsvendor Problem with Parametric Operational Data Analytics (ODA)
Authors: Chu Leon Yang , Feng Qi , Shanthikumar J. George , Shen Zuo-Jun Max , Wu Jian 
Published: November 2024

We study the data-integrated, price-setting newsvendor problem in which the price–demand relationship is described by some parametric model with unknown parameters. We develop the operational data analytics (ODA) formulation of this problem that features a data-integration model and a validation model. The data-integration model consists of a class of functions called the operational statistics. Each operational statistic maps the available data to the ordering decision. The validation model finds, among the set of candidate operational statistics, the ordering decision that leads to the highest actual profit, which is unknown because of the unknown demand parameters. This ODA framework leads to a consistent estimate of the profit function with which we optimize the pricing decision. The derived quantity and price decisions demonstrate robust profit performance even when the sample size is very small in relation to the demand variability. Compared with the conventional approach with which the unknown parameters are estimated and then the decisions are optimized, the ODA framework produces significantly superior performance in the mean, standard deviation, and minimum of the profit, suggesting the robustness of the ODA solution especially in the small-sample regime. This paper was accepted by David Simchi-Levi, operations management. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2021.02227 .