bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arabidopsis DREB26/ERF12 and its close relatives regulate cuticular wax biosynthesis under drought stress condition
Authors: Urano Kaoru , Oshima Yoshimi , Ishikawa Toshiki , Kajino Takuma , Sakamoto Shingo , Sato Mayuko , Toyooka Kiminori , Fujita Miki , Kawai‐Yamada Maki , Taji Teruaki , Maruyama Kyonoshin , Yamaguchi‐Shinozaki Kazuko , Shinozaki Kazuo 
Published: October 2024

SUMMARYLand plants have evolved a hydrophobic cuticle on the surface of aerial organs as an adaptation to ensure survival in terrestrial environments. Cuticle is mainly composed of lipids, namely cutin and intracuticular wax, with epicuticular wax deposited on plant surface. The composition and permeability of cuticle have a large influence on its ability to protect plants against drought stress. However, the regulatory mechanisms underlying cuticular wax biosynthesis in response to drought stress have not been fully elucidated. Here, we identified three AP2/ERF transcription factors (DREB26/ERF12, ERF13 and ERF14) involved in the regulation of water permeability of the plant surface. Transmission electron microscopy revealed thicker cuticle on the leaves of DREB26‐overexpressing (DREB26OX) plants, and thinner cuticle on the leaves of transgenic plants expressing SRDX repression domain‐fused DREB26 (DREB26SR). Genes involved in cuticular wax formation were upregulated in DREB26OX and downregulated in DREB26SR. The levels of very‐long chain (VLC) alkanes, which are a major wax component, increased in DREB26OX leaves and decreased in DREB26SR leaves. Under dehydration stress, water loss was reduced in DREB26OX and increased in DREB26SR. The erf12/13/14 triple mutant showed delayed growth, decreased leaf water content, and reduced drought‐inducible VLC alkane accumulation. Taken together, our results indicate that the DREB26/ERF12 and its closed family members, ERF13 and ERF14, play an important role in cuticular wax biosynthesis in response to drought stress. The complex transcriptional cascade involved in the regulation of cuticular wax biosynthesis under drought stress conditions is discussed.
Ageing leads to selective type II myofibre deterioration and denervation independent of reinnervative capacity in human skeletal muscle
Authors: Horwath Oscar , Moberg Marcus , Edman Sebastian , Philp Andrew , Apró William 
Published: October 2024

AbstractAge‐related loss of muscle mass and function is underpinned by changes at the myocellular level. However, our understanding of the aged muscle phenotype might be confounded by factors secondary to ageing per se, such as inactivity and adiposity. Here, using healthy, lean, recreationally active, older men, we investigated the impact of ageing on myocellular properties in skeletal muscle. Muscle biopsies were obtained from young men (22 ± 3 years, n = 10) and older men (69 ± 3 years, n = 11) matched for health status, activity level and body mass index. Immunofluorescence was used to assess myofibre composition, morphology (size and shape), capillarization, the content of satellite cells and myonuclei, the spatial relationship between satellite cells and capillaries, denervation and myofibre grouping. Compared with young muscle, aged muscle contained 53% more type I myofibres, in addition to smaller (−32%) and misshapen (3%) type II myofibres (P < 0.05). Aged muscle manifested fewer capillaries (−29%) and satellite cells (−38%) surrounding type II myofibres (P < 0.05); however, the spatial relationship between these two remained intact. The proportion of denervated myofibres was ∼2.6‐fold higher in old than young muscle (P < 0.05). Aged muscle had more grouped type I myofibres (∼18‐fold), primarily driven by increased size of existing groups rather than increased group frequency (P < 0.05). Aged muscle displayed selective deterioration of type II myofibres alongside increased denervation and myofibre grouping. These data are key to understanding the cellular basis of age‐related muscle decline and reveal a pressing need to fine‐tune strategies to preserve type II myofibres and innervation status in ageing populations.
Neuromuscular mechanisms for the fast decline in rate of force development with muscle disuse – a narrative review
Authors: Ruggiero Luca , Gruber Markus 
Published: October 2024

AbstractThe removal of skeletal muscle tension (unloading or disuse) is followed by many changes in the neuromuscular system, including muscle atrophy and loss of isometric maximal strength (measured by maximal force,Fmax). Explosive strength, i.e. the ability to develop the highest force in the shortest possible time, to maximise rate of force development (RFD), is a fundamental neuromuscular capability, often more functionally relevant than maximal muscle strength. In the present review, we discuss data from studies that looked at the effect of muscle unloading on isometric maximalversusexplosive strength. We present evidence that muscle unloading yields a greater decline in explosive relative to maximal strength. The longer the unloading duration, the smaller the difference between the decline in the two measures. Potential mechanisms that may explain the greater decline in measures of RFD relative toFmaxafter unloading are higher recruitment thresholds and lower firing rates of motor units, slower twitch kinetics, impaired excitation‐contraction coupling, and decreased tendon stiffness. Using a Hill‐type force model, we showed that this ensemble of adaptations minimises the loss of force production at submaximal contraction intensities, at the expense of a disproportionately lower RFD. With regard to the high functional relevance of RFD on one hand, and the boosted detrimental effects of inactivity on RFD on the other hand, it seems crucial to implement specific exercises targeting explosive strength in populations that experience muscle disuse over a longer time.image
The COVID Pandemic Worsened Physical Activity Disproportionately in Frailer Adults: A Canadian Longitudinal Study on Aging
Authors: O’Brien Myles , Mayo Andrea , Theou Olga 
Published: October 2024

Introduction: Frailty is associated with increased hospitalization and mortality and may be exacerbated with physical inactivity. The COVID-19 pandemic has heterogeneously impacted peoples’ physical activity level, but the impact on the frail population is unclear. Objective: The objective of this study is to test the hypothesis that higher frailty levels were associated with worse changes in physical activity, ability to move around the home, and do housework during the pandemic. Methods: We included 23,303 Canadians (age: 69.1 ± 9.5 years, 53% females) who participated in the Canadian Longitudinal Study on Aging COVID-19 Questionnaire study. Frailty prior to the pandemic (follow-up 1: 2015–2018) was measured via 52-item frailty index and grouped into 0.00–0.05, 0.05–0.10, 0.10–0.20, and >0.20 scores. Participants were asked whether they changed their levels of physical activity, ability to move at home, and ability to do housework during the pandemic. Results: The average frailty level was 0.09 ± 0.06. Compared with the lowest frailty group, participants with frailty levels >0.20 reported worse physical activity (17% vs. 45%), worse ability to move at home (3% vs. 26%), and perform housework (3% vs. 27%). Covariate-adjusted logistic regression models demonstrated that higher frailty level was associated with a greater odds of reporting worse physical activity (reference: 0.00–0.05; odds ratio for frailty index >0.20:4.03, 95% confidence interval [3.33, 4.87]), worse ability to move in home (odds ratio  = 11.16, 95% confidence interval [8.28, 15.03]), and worse ability doing housework (odds ratio  = 12.58, 95% confidence interval [9.24, 17.13]). Conclusions: The adverse changes in physical activity levels and ability to move at home among frail older adults during the pandemic document the need for strategies to help vulnerable populations move more.
Association Between Physical Activity and Performance in Skill Learning Among Older Adults Based on Cognitive Function
Authors: Kasahara Satoshi , Chiba Ami , Jiang Linjing , Ishida Tomoya , Koshino Yuta , Samukawa Mina , Saito Hiroshi , Tohyama Harukazu 
Published: October 2024

Background/Objectives: Most older adults experience cognitive and physical functioning problems; however, they require the ability to learn skills in response to age-related or social environmental changes for independent living. This study aimed to clarify the associations between age-related physical activity and performance in skill learning tasks based on cognitive function. Methods: Fifty-eight adults participated in this study and were divided into two groups: the control group (aged under 65 years) and older adult group (aged over 65). All the participants performed two-skill learning exercises based on cognitive function. Habitual exercise was measured using an accelerometer and a self-reported questionnaire. Results: At baseline, the scores on skill tasks were lower in the older adult group than in the control group and were associated with habitual exercise and motor performance. Skill acquisition, observed in both groups, was associated with age and self-reported physical activity. Retention of the acquired skill was not associated with habitual exercise, and it declined significantly in the older group. Conclusions: Skill acquisition was maintained regardless of age; however, the ability to retain the acquired skills decreased among the older adults. Habitual physical activity was associated with skill acquisition but not the retention of the acquired skill. Significance/Implications: The study findings highlight the association between habitual exercise and motor skill learning in older adults, providing insight for practitioners in the rehabilitation and health care fields.
Director Selection: Drivers for the Adoption and Design of Nomination Committees by New Zealand National Sport Organizations
Authors: Molloy Tracy , Dickson Geoff , Ferkins Lesley 
Published: October 2024

Nomination committees (NCs) are a critical, yet under-researched, part of the good governance equation. This study contributes to baseline knowledge of NC adoption and design. Underpinned by critical realism, four national sports organization case studies explain the “why” and “how” of NC adoption through a multitheoretical lens. Change strategies are identified using Hampel et al.’s mechanisms (symbolic, relational, and material) approach to institutional (creation) work outcomes. Archer’s morphogenetic cycle helps to demonstrate the interplay between structure, culture, and agency in achieving the change with the study, providing a timely reminder of the power of morphostasis (inertia). The results are important for future NC design to better inform national sport organization NCs’ structures and processes (including case-appropriate balance between community and corporate logics in national sport organization governance re-configurations) and aid future evaluations of NC effectiveness. A critical realism multitheoretical and multicase approach is modeled for future sport management studies.
A new selective force driving metabolic gene clustering
Authors: Fondi Marco , Pini Francesco , Riccardi Christopher , Gemo Pietro , Brilli Matteo 
Published: October 2024

ABSTRACT

The evolution of operons has puzzled evolutionary biologists since their discovery, and many theories exist to explain their emergence, spreading, and evolutionary conservation. In this work, we suggest that DNA replication introduces a selective force for the clustering of functionally related genes on chromosomes, which we interpret as a preliminary and necessary step in operon formation. Our reasoning starts from the observation that DNA replication produces copy number variations of genomic regions, and we propose that such changes perturb metabolism. The formalization of this effect by exploiting concepts from metabolic control analysis suggests that the minimization of such perturbations during evolution could be achieved through the formation of gene clusters and operons. We support our theoretical derivations with simulations based on a realistic metabolic network, and we confirm that present-day genomes have a degree of compaction of functionally related genes, which is significantly correlated to the proposed perturbations introduced by replication. The formation of clusters of functionally related genes in microbial genomes has puzzled microbiologists since their first discovery. Here, we suggest that replication, and the copy number variations due to the replisome passage, might play a role in the process through a perturbation in metabolite homeostasis. We provide theoretical support to this hypothesis, and we found that both simulations and genomic analysis support our hypothesis.

IMPORTANCE
The formation of clusters of functionally related genes in microbial genomes has puzzled microbiologists since their discovery. Here, we suggest that replication, and the copy number variations due to the replisome passage, might play a role in the process through a perturbation in metabolite homeostasis. We provide theoretical support to this hypothesis, and we found that both simulations and genomic analysis support our hypothesis.
Determination of the Dipole Moment Variation Upon Excitation in the Chromophore of Green Fluorescent Protein From Molecular Dynamic Trajectories with QM/MM Potentials Using Machine Learning Methods
Authors: Zakharova T. M. , Kulakova A. M. , Krinitsky M. A. , Varentsov M. I. , Khrenova M. G. 
Published: October 2024

Abstract
Quantum and molecular mechanics (QM/MM) potentials are used to calculate molecular dynamics trajectories for the EYFP protein of the green fluorescent protein family. Machine learning models are constructed to establish the relationship between the geometric parameters of the chromophore in the frame of its trajectory and the properties of its electronic excitation. It is shown that it is not enough to use only bridging bonds between the phenyl and imidazolidone fragments of the chromophore as a geometric parameter, and at least two more neighboring bonds must be added to the model. The proposed models allow determination of the dipole moment variation upon excitation with an average error of 0.11 a.u.
40Ar/39Ar Dating of Hydrothermal Processes in Large Gold Deposits of the Kochkar Anticlinorium (South Urals, Russia)
Authors: Pritchin M. E. , Kisin A. Yu. , Vikent’eva O. V. , Ozornin D. A. , Travin A. V. , Vikentyev I. V. 
Published: October 2024

Abstract
The age restrictions for productive mineral associations of the two largest gold deposits of the Southern Urals—Svetlinsk and Kochkar, which are located in the East Uralian megazone—are discussed. The 40Ar/39Ar dating method was performed for the first time for potassium-containing hydrothermal minerals (micas and amphiboles) from the ore veins and ore-host alteration, as well as from marbles. The age estimates obtained are in the range of 290–276 Ma; the weighted average value for the Svetlinsk deposit is 276 ± 2 Ma; for the Kochkar field, 284 ± 2 Ma. It is supposed that the studied mineral assemblages of the Svetlinsk and Kochkar deposits were formed in the beginning of the postcollision stage corresponding to the tectonic relaxation regime. Age of hydrothermal mineral formation in the gold fields of the Kochkar anticlinorium are consistent with the time of post-tectonic plutonic activity, which was expressed in the Middle–Southern Urals in large-scale granitization (~300 Ma). The ore-bearing alteration of a femic profile (of the same age for two deposits), which are most likely basificates, are near-synchronous with the granitization.
Haptoglobin polymorphism, vitamin E and mortality: the Ludwigshafen Risk and Cardiovascular Health Study
Authors: Delgado Graciela E , Kleber Marcus E , Moissl Angela P , Winklhofer-Roob Brigitte M , Krämer Bernhard K , Renner Wilfried , Langsenlehner Tanja , Dschietzig Thomas B , März Winfried , Armbruster Franz P 
Published: October 2024

ObjectiveIn humans, haptoglobin (Hp) exists in two allelic forms, Hp1 and Hp2, that differ significantly in their ability to protect the organism from oxidative stress. It has been proposed that in patients with diabetes mellitus carriers of the Hp2-2 genotype may benefit from vitamin E supplementation. Aim of our study was to investigate if there is evidence regarding a potential interaction between the Hp polymorphism and vitamin E with regard to mortality in individuals at medium-to-high cardiovascular risk with and without diabetes mellitus.Research design and methodsData from 3176 participants of the Ludwigshafen Risk and Cardiovascular Health study, a monocentric hospital-based study of patients referred for coronary angiography, were analysed using Cox proportional hazard regression.ResultsParticipants with the Hp2-2 genotype demonstrated significantly lower Hp levels, while carriers of at least one Hp-2 allele displayed elevated levels of the inflammatory markers high-sensitive C reactive protein and serum amyloid A. No notable differences in comorbidities were observed among the various HP genotype groups. While the HP genotype showed no direct association with mortality, a borderline significant correlation between α-tocopherol plasma concentration and overall mortality was noted. An interaction between vitamin E status and the HP genotype regarding mortality risk was evident, particularly among patients with diabetes mellitus, with a p value of 0.021 for the interaction term. In restricted cubic splice analysis, patients with diabetes mellitus who are carriers of the Hp2-2 genotype seem to benefit from higher γ-tocopherol concentrations whereas for the other genotype groups there was a direct association with mortality risk.ConclusionParticularly in patients with diabetes mellitus we could show a significant interaction of γ-tocopherol plasma concentration and HP genotype. Carriers of the Hp2-2 genotype seemed to benefit from higher plasma concentrations of γ-tocopherol. Further research is warranted to elucidate the underlying mechanisms and potential therapeutic implications in cardiovascular disease management.
Right ventricular remodelling and long-term survival after pulmonary endarterectomy versus balloon pulmonary angioplasty in chronic thromboembolic pulmonary hypertension
Authors: Ravnestad Håvard , Murbræch Klaus , Gjønnæss Eyvind , Andersen Rune , Moe Natasha , Birkeland Sigurd , Svalebjørg Morten , Lingaas Per Snorre , Gude Einar , Gullestad Lars , Kvitting John-Peder Escobar , Broch Kaspar , Andreassen Arne K 
Published: October 2024

Background
Pulmonary endarterectomy (PEA) is the treatment of choice for chronic thromboembolic pulmonary hypertension (CTEPH), while balloon pulmonary angioplasty (BPA) is an alternative for inoperable patients. We aimed to compare right ventricular (RV) remodelling and late survival after PEA and BPA.

Methods
In this prospective observational cohort study, we performed echocardiography at baseline and follow-up in patients with CTEPH treated with PEA (n=54) or BPA (n=44) between 2011 and 2022.

Results
Follow-up echocardiography was performed at 5 months (IQR 4–7) after PEA and 3 months (IQR 2–4) after the last BPA. Both groups showed significant improvements in left ventricular end-systolic eccentricity index, RV basal diameter and RV fractional area change (RV FAC). Tricuspid regurgitation pressure decreased by 26±18 mm Hg after PEA and 13±21 mm Hg after BPA (p=0.02 for between-group difference). Tricuspid annular systolic excursion (TAPSE) decreased by 4±5 mm after PEA but increased by 1±4 mm after BPA (p<0.001). The TAPSE/systolic pulmonary artery pressure ratio improved similarly in both groups. Five-year survival was 96% (95% CI 86% to 99%) for PEA and 79% (95% CI 61% to 89%) for BPA (p=0.25). Change in RV FAC was an independent predictor of survival (HR 0.9, 95% CI 0.82 to 0.99, p=0.03).

Conclusions
Both PEA and BPA led to significant RV reverse remodelling, with no clear evidence of a difference in survival rates. Improvement in RV function, particularly RV FAC, was associated with better outcomes, highlighting the importance of RV recovery in CTEPH treatment.
Solar neutrinos in cryogenic detectors
Authors: Bento A. , Bertolini A. , Canonica L. , Di Lorenzo S. , Dominsky F. , Iachellini N. Ferreiro , Fuchs D. , Garai A. , Hauff D. , Langenkämper A. , Mancuso M. , Mauri B. , Petricca F. , Pröbst F. , Pucci F. , Stodolsky L. 
Published: October 2024

AbstractCoherent elastic neutrino-nucleus scattering (CE$$\nu $$
ν
NS) poses an irreducible background in the search for dark matter-nucleus elastic scatterings, which is commonly known as the neutrino floor. As direct dark matter search experiments keep improving their sensitivity into so far unexplored regions, they face the challenge of approaching this neutrino floor. A precise description of the CE$$\nu $$
ν
NS signal is therefore crucial for the description of backgrounds for future DM searches. In this work we discuss the scenario of detecting neutrinos in low-threshold, high-exposure cryogenic solid state experiments optimized for the search of low-mass dark matter. The energy range considered is completely dominated by solar neutrinos. In absence of any dark matter events, we treat solar neutrinos as the main signal of interest. We show that sensitivity to the flux of neutrinos from different production mechanisms can be achieved. In particular we investigate the sensitivity to the flux of pp and $$^{7}$$

7

Be neutrinos, as well as CNO neutrinos. Furthermore, we investigate the sensitivity to dark matter signals in the presence of a solar neutrino background for different experimental scenarios, which are defined by three parameters: the target material, the energy threshold and the exposure. We show that experiments with thresholds of $$\mathcal {O}$$
O
(eV) and exposures of $$\mathcal {O}$$
O
(tonne-years), using $$\hbox {CaWO}_{4}$$

CaWO
4

or $$\hbox {Al}_{2}\hbox {O}_{3}$$

Al
2

O
3

targets, have discovery potential for dark matter interaction cross sections in the neutrino floor.
Path Planning in Payment Channel Networks with Multi-Party Channels
Authors: Corcoran Padraig , Lewis Rhyd 
Published: October 2024

Payment Channel Networks (PCNs) provide a means to improve the scaling of cryptocurrency payments by allowing peers to make payments between themselves in an efficient manner. To make a payment between two peers, the task of path planning must first be performed to determine a path in the PCN connecting the peers in question before the payment in question is performed using this path. To date, existing research has focused on the problem of performing path planning in PCNs that contain two-party channels. It has been hypothesised that the scaling of PCNs could be further improved by considering the inclusion of multi-party channels that contain more than two peers. However, the problem of performing path planning in PCNs that contain multi-party channels has not yet been considered.
In this article, we address this gap in the research literature and propose a novel path planning method for PCNs containing multi-party channels. This method involves modelling the PCN with multi-party channels as a hypergraph, a type of graph where edges can contain two or more vertices, and using this model to solve the path planning problem in question. We prove that the proposed method is correct and computationally efficient. Furthermore, assuming path planning is performed using this method, we also present theoretical and experimental analyses that demonstrate the scaling benefits of using multi-party channels.