bluuebunny/crossref_metadata_2025_split huggingface dataset:
NFAT1 protects articular cartilage against osteoarthritic degradation by directly regulating transcription of specific anabolic and catabolic genes
Authors: Zhang M. , Lu Q. , Budden T. , Wang J. 
Published: February 2019

Objectives Adult mice lacking the transcription factor NFAT1 exhibit osteoarthritis (OA). The precise molecular mechanism for NFAT1 deficiency-induced osteoarthritic cartilage degradation remains to be clarified. This study aimed to investigate if NFAT1 protects articular cartilage (AC) against OA by directly regulating the transcription of specific catabolic and anabolic genes in articular chondrocytes. Methods Through a combined approach of gene expression analysis and web-based searching of NFAT1 binding sequences, 25 candidate target genes that displayed aberrant expression in Nfat1-/- AC at the initiation stage of OA, and possessed at least four NFAT1 binding sites in the promoter of each gene, were selected and tested for NFAT1 transcriptional activities by chromatin immunoprecipitation (ChIP) and promoter luciferase reporter assays using chondrocytes isolated from the AC of three- to four-month-old wild-type mice or Nfat1-/- mice with early OA phenotype. Results Chromatin immunoprecipitation assays revealed that NFAT1 bound directly to the promoter of 21 of the 25 tested genes encoding cartilage-matrix proteins, growth factors, inflammatory cytokines, matrix-degrading proteinases, and specific transcription factors. Promoter luciferase reporter assays of representative anabolic and catabolic genes demonstrated that NFAT1-DNA binding functionally regulated the luciferase activity of specific target genes in wild-type chondrocytes, but not in Nfat1-/- chondrocytes or in wild-type chondrocytes transfected with plasmids containing mutated NFAT1 binding sequences. Conclusion NFAT1 protects AC against degradation by directly regulating the transcription of target genes in articular chondrocytes. NFAT1 deficiency causes defective transcription of specific anabolic and catabolic genes in articular chondrocytes, leading to increased matrix catabolism and osteoarthritic cartilage degradation. Cite this article: M. Zhang, Q. Lu, T. Budden, J. Wang. NFAT1 protects articular cartilage against osteoarthritic degradation by directly regulating transcription of specific anabolic and catabolic genes. Bone Joint Res 2019;8:90–100. DOI: 10.1302/2046-3758.82.BJR-2018-0114.R1.
Penerapan suhu pemingsanan dalam transportasi sistem kering ikan baronang Siganus sp.
Authors: Hidayat Taufik , Bramantyo Muhammad Baiquni , Nurilmala Mala 
Published: February 2019

Abstract. Rabbitfish (Siganus sp.) is an unfamiliar fish consumption in public. Demand of fresh fishes continue to increase every year. One method to maintain freshness of fish is fish transportation method. The research purpose was to determine the best rabbitfih immotilization temperature for filler media in transportation tools packaging, immotilization fish use 2 ppt of clove oil, and then fish conscious test was measured at , 14°C, dan 17°C, and 19°C of temperatures. The experiments were the fish conscious time and water quality analyses. Test of water qualities were seawater before used, water plus anesteshia (water+clove oil), immotilization water (water+clove oil+fish), seawater for temperature treatment, and seawaterafter conscious fish. Water quality parameter analyses were DO (Dissolved oxygen),pH, CO2, TAN, KH, GH, NO3, NO2. The best temperature of fish conscious time test in this experiment was 17°C with 300 minutes of conscious time.Keywords: Immotilization; Siganus sp.; Transportation; Water quality Abstrak. Ikan baronang (Siganus sp.) merupakan ikan konsumsi yang belum familiar di masyarakat luas. Ikan segar terus meningkat permintaannya setiap tahunnya. Salah satu metode untuk mempertahankan kesegaran ikan adalah dengan metode transportasi. Penelitian ini bertujuan untuk mengetahui suhu pemingsanan terbaik ikan baronang untuk media pengisi pada kemasan alat transportasi, ikan dipingsankan dengan minyak cengkeh 2 ppt kemudian di uji waktu sadar ikan dengan perlakuan suhu 19°C, 14°C, dan 17°C. Pengujian yang dilakukan adalah uji waktu sadar ikan dan uji kualitas air. Kualitas air yang diuji adalah air laut sebelum digunakan, air ditambah anestesi (air+minyak cengkeh), air proses pemingsanan (air+minyak cengkeh+ikan), air laut untuk perlakuan suhu, dan air setelah pembugaran.Parameter uji kualitas air adalah DO, pH, CO2, TAN, KH, GH, NO3, NO2. Pengujian waktu sadar ikan terbaik adalah dengan perlakuan suhu 17°C dengan waktu sadar sebesar 300 menit. Kata kunci:Transportasi;Siganussp.; Pemingsanan; Kualitas air
Akumulasi logam berat (Pb dan Cu) pada Akar dan daun mangrove Avicennia marina di Sungai Lamong, Jawa Timur
Authors: Awaliyah Hikmah Farda , Yona Defri , Pratiwi Dwi Candra 
Published: February 2019

Abstract. Mangrove ecosystem of Lamong River is located in an area with dense human activities such as industrial, heavy traffic, settlements and harbour activities. These activities could triggered the input of heavy metals, especially Pb and Cu. Mangrove ecosystem can be developed as a control of heavy metal pollution and also as a pollutant trap. In this study, the analysis of heavy metals (Pb and Cu) in water, sediment and mangorve’s roots and leaves were measured. The aim of this study was to determine the ability of Avicennia marina’s roots and leaves in absorbing heavy metals (Pb and Cu). Samples were obtained and then analysed by AAS (Atomic Absorbsion Spectrofotometer).The result showed that heavy metal concentrations of Pb were in the range of 0.11 mg/L - 16.87 mg/L and Cu in the range of 0.001 mg/L - 34.84 mg/L.Avicennia marina in the study site was categorized as excluder based on BCF1 and phytoextraction due to TF1. The results of this study indicate that Avicennia marina can be developed into phytoremidiation agents because they was able to absorb and transfer heavy metals from the environment to their plant tissues.Keywords: BCF; TF; Avicennia marina; Heavy metal; Lamong River Abstrak. Ekosistem mangrove Sungai Lamong terletak di area yang padat aktivitas manusia seperti aktivitas industri, lalu lintas yang padat, pemukiman dan pelabuhan. Hal tersebut memicu masukan logam berat, khususnya Pb dan Cu. Ekosistem mangrove dapat dikembangkan sebagai pengendalian pencemaran logam berat dan dapat juga digunakan sebagai pollutant trap. Pada penelitian ini bertujuan untuk mengetahui kemampuan Avicennia marinadalam menyerap logam berat Pb dan Cu dari lingkungannya. Sampel dianalisa menggunakan AAS (Atomic Absorbsion Spectrofotometer).Hasil konsentrasi logam berat Pb berkisar antara 0,11 mg/L – 16.87 mg/L dan Cu berkisar antara 0,001 mg/L – 34,84 mg/L. Avicennia marinadi lokasi penelitian bersifat excluderkarena nilai BCF1 dan fitoekstraksi karena nilai TF1. Hasil penelitian ini menunjukkan bahwa mangrove Avicennia marinadapat dikembangkan menjadi agen fitoremidiasi karena mampu menyerap dan mentransfer logam berat dari lingkungan ke jaringan tubuh yang lain.Kata kunci:BCF; TF; Avicennia marina; Logam berat; Sungai Lamong
DAYA TARIK BUNGA SAKURA BAGI MASYARAKAT JEPANG
Authors: Hastuti Nur 
Published: February 2019

Sakura is the pride flower of Japanese from the past. Many person can enjoy the beauty of Sakura. Sakura usually blossoms in the spring season, begins from south Japan region in the middle of March and continue to the norh Japan ( in the middle of April up to end of April). During spring season, mass media reports about Zensen Sakura, that is prediction the growth of Sakura in many places in Japan, along with climate prediction. By that information, Japanese people are ready to welcome warmer air in the spring ( after winter) and holding Hanami feast. Based on that situation, writer interest to write this research by the title “ The Attraction of Sakura Flower for Japanese people”. The problem that will be analized is how is the attraction and the effect of Sakura flower for Japanese people. In this research, writer uses literary review from internet, article, and many other sources. The attraction of Sakura flower is Japanese people can enjoy Hanami with family, friends, and close person in the office and organization. The effect of Sakura in Japanese life is very important, like to raise the affection in the family, make closer the friendship,and make better communication with partner or relation.
On viscosity and weak solutions for non-homogeneous p-Laplace equations
Authors: Medina Maria , Ochoa Pablo 
Published: February 2019

Abstract
In this manuscript, we study the relation between viscosity and weak solutions for non-homogeneous p-Laplace equations with lower-order term depending on x, u and

{\nabla u}

. More precisely, we prove that any locally bounded viscosity solution constitutes a weak solution, extending results presented in Juutinen, Lindqvist and Manfredi [9], and Julin and Juutinen [6]. Moreover, we provide a converse statement in the full case under extra assumptions on the data.
Integrating Bayesian reasoning into medical education using smartphone apps
Authors: Kinnear Benjamin , Hagedorn Philip A. , Kelleher Matthew , Ohlinger Courtney , Tolentino Jonathan 
Published: February 2019

Abstract

Background
Diagnostic reasoning is an important topic in medical education, and diagnostic errors are increasingly recognized as large contributors to patient morbidity and mortality. One way to improve learner understanding of the diagnostic process is to teach the concepts of Bayesian reasoning and to make these concepts practical for clinical use. Many clinician educators do not fully understand Bayesian concepts and they lack the tools to incorporate Bayesian reasoning into clinical practice and teaching.

Methods
The authors developed an interactive workshop using visual models of probabilities and thresholds, clinical cases, and available smartphone apps to teach learners about Bayesian concepts.

Results
Evaluations from 3 years of workshops at a national internal medicine chief resident conference showed high satisfaction, with narrative comments suggesting learners found the visual and smartphone tools useful for applying the concepts with future learners.

Conclusions
Visual models, clinical cases, and smartphone apps were well received by chief residents as a way to learn and teach Bayesian reasoning. Further study will be needed to understand if these tools can improve diagnostic accuracy or patient outcomes.
Cellulose Acetate Mixed Matrix Membranes Coated with PEG/TiO2 for Removal of Pb(II) Ions from Aqueous Solutions: Combined Experimental and Quantum Chemical Modeling Investigation
Authors: Nouri Mohammad , Marjani Azam , Tajdari Majid 
Published: February 2019

Abstract
Porous cellulose acetate (CA) flat sheet membranes containing 8-hydroxyquinoline (as complexing agent) coated with polyethylene glycol (PEG)/TiO2 nanocomposite solution were fabricated. For the removal of Pb(II) ions from aqueous solutions, retention data were measured experimentally. To analyze and examine the practical effect of the coating layer on the rejection of metal ions, a quantum chemical calculation and modeling approach was employed. The simulations were conducted on (1) coating layers and (2) a substrate layer. In each simulation, (i) the lowest energy sites on layers were searched against their affinity for metal ions by performing Metropolis Monte Carlo and configurational bias Monte Carlo search of the ion–membrane configurational space according to a simulated annealing schedule, and (ii) the evolution of ions in each layer was examined by the mean square displacement through molecular dynamics simulations. The coating layer showed a potential to significantly interact with ions with higher energy requirements for the detachment from the coating layer in comparison to the substrate layer. This suggested that the metal ions would increasingly accumulate on the coating layer and its lateral surface. The mean square displacement data showed smaller values in the coating layer rather than in the substrate layer, indicating that the coating layer efficiently inhibits ion transfer and provides higher retention values. Thus, greater metal ion retention is expected on and around the coating layer. This is in agreement with the determined attachment and detachment energies. Finally, acceptable agreement was found between numerical and experimental results.
Typical characteristics of children with congenital adrenal hyperplasia due to 11β-hydroxylase deficiency: a single-centre experience and review of the literature
Authors: Breil Thomas , Yakovenko Vira , Inta Ioana , Choukair Daniela , Klose Daniela , Mittnacht Janna , Schulze Egbert , Alrajab Abdul , Grulich-Henn Jürgen , Bettendorf Markus 
Published: February 2019

Abstract

Background
11β-hydroxylase deficiency (11βOHD) is a rare disease representing the second most common cause of congenital adrenal hyperplasia (CAH) (5–8%) with an incidence of about 1:100,000. In contrast to 21-hydroxylase deficiency (21OHD), 11βOHD is not included in neonatal screening programmes. The objective of this study was to demonstrate the typical features of male patients with 11βOHD.

Methods
Clinical, biochemical and radiological data of patients with 11βOHD were analysed in this retrospective single-centre analysis.

Results
Six male patients of four unrelated families with 11βOHD were identified (0.1–13.5 years of chronological age [CA] at diagnosis). The predominant symptoms were arterial hypertension, tall stature and precocious pseudopuberty. Bone ages (BAs) were remarkably advanced at diagnosis in four index patients (median difference BA–CA: 5.5 years, range 1.5–9.2 years). Homozygous mutations were identified in exon 7 (c.1179_1180dupGA [p.Asn394Argfs*37]) and exon 8 (c.1398+2T>C) of the CYP11B1 gene leading both to a complete loss of function. The latter mutation has not yet been described in databases. 11βOHD was identified by the measurement of 11-deoxycortisol in a newborn screening card of one patient retrospectively. Testicular adrenal rest tumours (TARTs) were detected in three patients at 3.7 years, 11 years and 14.4 years.

Conclusion
The diagnosis of CAH due to 11βOHD is delayed and should be suspected in children with arterial hypertension, tall stature and precocious pseudopuberty. Patients may develop TARTs as early as infancy. 11βOHD should be included in newborn screening programmes, at least in newborns of index families, to allow early diagnosis and the start of treatment to reduce morbidity.
Evaluating the four most important salivary sex steroids during male puberty: testosterone best characterizes pubertal development
Authors: Krebs Andreas , Dickhuth Karoline , Mumm Rebekka , Stier Bernhard , Doerfer Jürgen , Grueninger Dirk , Wurm Michael , Brichta Corinna , Schwab Karl Otfried 
Published: February 2019

Abstract

Background
During pubertal development in healthy boys, increased levels of different sex steroids occur which are responsible for sexual maturation and physical changes. However, relationships between various sex hormones and pubertal development stages have not been sufficiently studied.

Methods
The investigation included 165 normal boys (mean age 12.7±2.8 years, mean body mass index [BMI] 19.6±4.2 kg/m2). Pubic hair (PH) stages were stratified by Tanner and testicular volume (TV) by means of the Prader orchidometer and assigned to the prepubertal, pubertal and postpubertal development phase. Four different sex steroids (testosterone [TE], dehydroepiandrosterone [DHEA]/dehydroepiandrosterone-sulfate [DHEAS], androstenedione (AE), 17-hydroxyprogesterone [17-OHP]) were measured in saliva by enzyme-linked immunosorbent assay (ELISA) and as serum total steroids by different assays (radioimmunoassay [RIA], chemiluminescence immunoassay [CLIA], electrochemiluminescence immunoassay [ECLIA]). Validation of saliva-based ELISA tests included data related to inter- and intra-assay coefficients of variation (CVs), recovery and linearity.

Results
Using Spearman rank correlation, salivary steroids significantly correlated (p<0.001) with pubertal development: TE (TV r=0.74 and PH stages r=0.72), DHEA (r=0.58 and 0.62), AE (r=0.38 and 0.45) and 17-OHP (r=0.42 and 0.43). Correlations between salivary and serum concentrations of steroids were also statistically significant (p<0.001). Binomial logistic regression analysis revealed significant correlations between salivary TE and pubertal maturation during the development phases of prepuberty-puberty and puberty-postpuberty. Inclusion of further salivary steroids did not improve analysis results.

Conclusions
Salivary TE permits a good non-invasive characterization of pubertal maturation stages. The consideration of further salivary sex steroids did not improve diagnostic accuracy.
Automatic event detection in basketball using HMM with energy based defensive assignment
Authors: Keshri Suraj , Oh Min-hwan , Zhang Sheng , Iyengar Garud 
Published: February 2019

Abstract
We propose a unsupervised learning framework for automatically labeling events in a basketball game. Our framework uses the the optical player tracking data in the NBA. We first learn the time series of defensive assignments using a novel player and location dependent attraction based model which uses hidden Markov models (HMMs), Gaussian processes, and a “bond breaking” model for changes in defensive assignments. Next, we use the learned defensive assignments as an input to a set of HMMs that automatically detect events such as ball screens, drives and post-ups. We show that our models provide significant improvements over existing benchmarks both on defensive assignments and event detection.
Interleukin-13 in the pathogenesis of pulmonary artery hypertension
Authors: Yuan Shi-Min 
Published: February 2019

Abstract

Background
Interleukin (IL)-13 is a regulatory factor of tissue remodeling and is involved in the pathogenesis of pulmonary artery hypertension (PAH). However, the implications of IL-13 in PAH remains uncertain. This article aims to describe the current knowledge on production and function of IL-13 and its receptors in the mechanisms of PAH.

Content
The study materials of this article were based on comprehensive literature retrieval of publications of IL-13 in PAH. These study materials were carefully reviewed, analyzed and discussed.

Summary
IL-13 levels in blood and lung tissue were elevated in both animal models of PAH and patients with PAH in comparison to non-PAH controls. Types I and II IL-13 receptors participate in pulmonary artery remodeling through signal transducer and activator of transcription (STAT)6 or through phosphatidylinositol 3-kinase (PI3K), STAT3 and mitogen activated protein kinase (MAPK) pathways. Oxidant, arginase 2 (Arg2) and hypoxia-inducible factor 1α are involved in the proliferation of pulmonary artery smooth muscle cells.

Outlook
Types I and II IL-13 receptors play an important role in the IL-13 signaling by STAT6 via Janus kinase kinases, and by PI3K, STAT3 and MAPK pathways, respectively. Alternative pathways, including oxidant, Arg2 and hypoxia-inducible factor 1α might be also involved in the pathological process of PAH development. Investigational therapies by inflammatory suppression or thrombolytic and anticoagulant agents could inhibit intimal hyperplasia of the pulmonary arteries and suppress pulmonary vasculature remodeling. Drug research and development oriented by this hypothesis would confer benefits to the treatment of PAH.
Musculoskeletal clinical and imaging manifestations in inflammatory bowel diseases
Authors: Sarbu Mihaela Ionela , Sarbu Nicolae 
Published: February 2019

Abstract
Rheumatic musculoskeletal manifestations are increasingly recognized as a major cause of morbidity and impaired quality of life in patients with inflammatory bowel diseases (IBDs). IBDs are associated with a variety of musculoskeletal pathologies, from peripheral arthritis to axial involvement, and from localized or regional pathologies to diffuse metabolic disorders. Recent advances, especially in imaging techniques, allow a better understanding of these pathologies, and assist their recognition even in the preclinical phase. This review aims to describe the musculoskeletal clinical and imaging manifestations in IBD with special emphasis on the current concepts and the updated radiological work-up.
Analysis of hormone receptor status in primary and recurrent breast cancer via data mining pathology reports
Authors: Chang Kai-Po , Chu Yen-Wei , Wang John 
Published: February 2019

Abstract

Background
Hormone receptors of breast cancer, such as estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (Her-2), are important prognostic factors for breast cancer.

Objective
The current study aimed to develop a method to retrieve the statistics of hormone receptor expression status, documented in pathology reports, given their importance in research for primary and recurrent breast cancer, and quality management of pathology laboratories.

Method
A two-stage text mining approach via regular expression-based word/phrase matching, was developed to retrieve the data.

Results
The method achieved a sensitivity of 98.8%, 98.7% and 98.4% for extraction of ER, PR, and Her-2 results. The hormone expression status from 3679 primary and 44 recurrent breast cancer cases was successfully retrieved with the method. Statistical analysis of these data showed that the recurrent disease had a significantly lower positivity rate for ER (54.5% vs 76.5%, p=0.001278) than primary breast cancer and a higher positivity rate for Her-2 (48.8% vs 16.2%, p=9.79e-8). These results corroborated the previous literature.

Conclusion
Text mining on pathology reports using the developed method may benefit research of primary and recurrent breast cancer.
The reliability of adjusting stepped care based on FeNO monitoring for patients with chronic persistent asthma
Authors: Wang Xiaoru , Wu Ling , Zhang Zhi , Kong Qinghua , Qi Hui , Lei Han 
Published: February 2019

Abstract

Objective
To examine the feasibility of fractional exhaled nitrous oxide (FeNO) guided stepped care in patients with chronic persistent asthma.

Methods
160 patients with asthma were enrolled and randomly divided into study and control groups, and were given standardized treatment according to GINA 2014. All patients were evaluated every 3 months and their medication was adjusted according to the results of evaluation. The control group was adjusted according to the recommended protocol from GINA, while the study group was adjusted on the basis of the control group and combined with the results of FeNO. The complete control rate, failure rate of stepwise treatment, ACQ score, lung function, and peripheral blood eosinophil count were compared between the two groups.

Results
In both study and control groups, the patient condition was effectively controlled. Strikingly, the failure rate of step therapy in study group was lower than that of control group (P<0.05), although there were no significant differences between the two groups on total control rate, ACQ score, lung function, and peripheral blood eosinophil count (P>0.05). Furthermore, the levels of FeNO positively correlated with ACQ scores and eosinophil counts or negatively with lung function.

Conclusions
The dynamic monitoring of FeNO could effectively guide the medication and reduce the rate of treatment failure, which could be used to inform standardized management of asthma.