bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibiotic susceptibility and resistance profiles of Romanian Clostridioides difficile isolates
Authors: Macovei Ioana , Lemeni Daniela , Șerban Roxana , Niculcea Andreea , Popescu Gabriel A. , Nica Maria , Petrini Anca , Mihăescu Grigore 
Published: May 2018

Abstract
This study investigated the antibiotic susceptibility patterns and genetic resistance markers of 35 C. difficile strains isolated from patients with C. difficile infection. Vancomycin, metronidazole, tigecycline, teicoplanin, rifampicin, moxifloxacin, cefotaxime, tetracycline, erythromycin, clindamycin, chloramphenicol, linezolid and imipenem MICs were determined for toxigenic strains belonging to PCR ribotypes (PR) 012 (2), 014 (4), 017 (3), 018 (2), 027 (17), 046 (2), 087 (3) and 115 (2). Results showed vancomycin, metronidazole, tigecycline and teicoplanin to be active against all isolates. High resistance rates were noticed against cefotaxime (n = 35), clindamycin (n = 33), imipenem (n = 31), moxifloxacin (n = 25), erythromycin (n = 25) and rifampicin (n = 22). Linezolid-resistance was found in three isolates (PR 017/2, PR 012/1), showing complex resistance (7-9 antibiotics). PR 012, 017, 018, 027 and 046 isolates (n = 26) were resistant to 5-9 antibiotics. Twelve resistance profiles (2-9 antibiotics) were detected. Rifampicin-moxifloxacin-cefotaxime-erythromycin-clindamycin-imipenem-resistance was predominant, being expressed by 18 strains (PR 027/17, PR 018/1). PCR results suggested tetracycline-resistance to be induced by the gene tetM. Three tetM-positive isolates (PRs 012, 046), were also tndX-positive, suggesting the presence of a Tn5397-like element. Only two MLSB-resistant strains (PR 012) had the ermB gene and chloramphenicol-resistance determinant catD was not detected, leaving room for further investigating resistance mechanisms. Multidrug resistance could be attributed to most analysed strains, underlining, once more, the impact of wide-spectrum antimicrobial over prescription, still a tendency in our country, on transmission of antimicrobial resistance and emergence of epidemic C. difficile strains generating outbreaks.
Sanger sequencing of MMR genes in a one-plate system
Authors: Negura Lucian , Negura Anca 
Published: May 2018

Abstract
Both incidence and mortality of colorectal cancer (CRC) in Romania have shown a continuous increase during the last decades. Hereditary Non-Polyposic Colorectal Cancer (HNPCC), also known as Lynch syndrome, is mainly attributable to mismatch repair (MMR) genes MSH2, MSH6, and MLH1. Individuals carrying germ-line mutations of these genes present high lifetime risk of colorectal and other cancers, compared to non-carriers. Oncogenetics is developed worldwide nowadays, for identifying hereditary predisposition to cancer and offering appropriate clinical follow-up to patients and mutation carriers in Lynch families. Molecular oncogenetic diagnosis in Lynch syndrome is based on complete Sanger sequencing of entire MMR genes, which is time and resources consuming, therefore needing an appropriate and adapted optimization. Conventional sequencing requires a sufficient number of available samples to be processed simultaneously, which increases the waiting time for diagnostic results. Complete analysis for only one patient meets difficult technical problems due to the complex co-amplification of all gene regions of interest within the same conditions, therefore increasing the costs and reducing the cost-effectiveness of the test. Here we present an original and robust technical protocol for sequencing the entire MSH2, MSH6, and MLH1 coding sequence for one patient in a single PCR plate. Our optimized and verified system overcomes all technical problems and offers a quick, robust, and cost-effective possibility to personalize molecular oncogenetic diagnosis in Lynch syndrome.
Antiphosphatidylethanolamine Antibodies and Deep Vein Thrombosis in Lupus Patients with Antiphospholipid Syndrome
Authors: Caraiola Simona , Jurcuț Ciprian , Dima Alina , Băicuș Cristian , Băicuș Anda 
Published: May 2018

Abstract
Backgroud. Which factors determine venous thrombotic events in some antiphospholipid syndrome (APS) patients and arterial thrombosis or conditions related to pregnancy in others has not been established yet. Purpose. The aim of this study was to search the antiphospholipid antibodies (APLAs) correlates in regard to deep vein thrombosis (DVT) in patients with systemic lupus erythematosus (SLE) and APS. Methods. Twenty-nine patients fulfilling the criteria of both SLE and APS were included. Complete anamnesis and clinical examination was performed on inclusion. Also, for all patients, disease activity was assessed by the SLEDAI score. An extended APLAs profile, ten Abs, was searched. Results. The titers of IgG anticardiolipin (aCL), IgG anti-β2 glycoprotein I (aβ2GPI), IgG antiphosphatidylethanolamine (aPE), and also of IgG antiprothrombin (aPT) were significant higher in patients with DVT history. After analysis by ROC curve and univariate logistic regression, the strongest association was found for IgG aPE. Also, in multivariate analysis, SLEDAI score correlated with the DVT antecedents. Conclusions. IgG aPE might be involved in DVT pathogenic pathways in patients with SLE and APS as their titers remain significantly higher in patients with previous DVT. Lupus patients with DVT events represent a subgroup of patients with more severe underlying pathology.
In Vitro Antimicrobial Activities of 6-Substituted-3(2H)- pyridazinone-2-acetyl-2- (substituted/nonsubstitutedbenzal/ acetophenone) Hydrazone Derivatives
Authors: Abiha Gul Bayram , Bahar Leyla , Utku Semra 
Published: May 2018

Abstract
Aim: In vitro antibacterial activity of 6-substituted-3(2H)-pyridazinone-2-acetyl-2-(substituted/nonsubstitutedbenzal/ acetophenone) hydrazone derivatives were tested in common species causing hospital-acquired infections. Material and Method: Antimicrobial activities of the compounds were performed by determining minimum inhibitory concentration (MIC) value against four Gram-positive, five Gram-negative and four Candida species fungi. Modified serial microdilution method was carried out. Reference strains of American Type Culture Collection (ATCC) were used. Results: In general, eleven compounds exhibited considerable activity. Comparatively, compound 3 exhibited strong activity against Enterobacter hormaechei and 5, 11 were the most active against Acinetobacter baumannii at 31.25 μg/mL. Compounds 1,2,3,4,8 and 10 were found to be as active as positive control ampicillin trihidrate against Stenotrophomonas maltophilia. On the other hand, compounds 1,2,3,4,7,8,9,10 and 11 showed strong antifungal activitiy as much as fluconazole against Candida tropicalis. Compound 1 was mostly active against Candida albicans, Candida glabrata, Candida parapsilosis and Candida tropicalis. It was also revealed that the antifungal activity of compounds 1, 6, 7, 8 and 9 were higher than the others. Compound 1 and 8 exhibited the best activity against Candida glabrata and Candida parapsilosis respectively. Conclusions: All tested compounds showed better activity against Gram-negative bacteria and yeast than Gram-positive bacteria. These compounds may be considered as alternative antimicrobial agents in the treatment of multiple drug resistant Gram-negative, Gram-positive bacteria and fungal pathogens. Especially, we suggested that Compound 1 and 8 might be a promising candidate of new antifungal agents
Development of Numerical Model and Comparison with the Analytical Model for Predicting the Wire Effects in Wire Electric Discharge Grinding
Authors: Madhavadev Parthiban , Vijayan Krishnaraj , Mouleeswaran Senthilkumar 
Published: May 2018

Abstract
This paper discusses the development of a numerical model and its comparison with the analytical model to predict the wire deflection in WEDG. The movement of wire between upper and lower guides creates vibration during the machining process which can create micro deflection of the wire. The Finite Element Analysis model [FEA] for the wire deflection is analyzed and compared with the analytical model. The deflection of the wire due to the input current may affect the machining of micro parts, which will be used for further research in micro machining and also will help us to choose the parameters such as current, spark gap, wire feed, and the speed of the workpiece during micro machining with WEDG. The FEA results for various forces were compared with the analytical results and found that the developed numerical model results are in good agreement.
Fem Simulation of Thermal Cycles During Laser Welding of Aluminium
Authors: Sejč Pavol , Világoš Tomáš 
Published: May 2018

Abstract
The submitted paper deals with the simulation of thermal cycles during laser welding of aluminum sheets using Finite Element Method (FEM). After the design and creation of the simulation model, the simulation results were verified by the thermal cycles measured during the experiment on real samples. Verification has shown that, despite the differences that have resulted from some simplifications, the proposed model corresponds to the actual behavior of the material used during the laser welding process.
Design Thinking In Pharmacy Education: Inspiring Creative Problem Solving in the Next Generation of Pharmacists
Authors: Pugliese Robert Salvatore , Girone Gianna 
Published: May 2018

Born from the world of product and service innovation, design thinking is gaining popularity as a method for introducing creative problem solving into the education of health professionals. Mindsets developed through practicing design thinking can help learners and educators address complex healthcare issues in a whole new way. This article aims to introduce the concepts of design thinking to the pharmacy educator, give examples of its use in pharmacy education, and discuss the value of including it in pharmacy education from both an educator and a student’s perspective.

Topic: Commentary
Optimalisasi Persentase Serat Bambu Terhadap Sifat Fisis Papan Komposit Beton Ringan
Authors: Mahyudin Alimin , Yunita Devi , Milvita Dian 
Published: May 2018

Telah dilakukan penelitian dengan judul optimalisasi serat bambu terhadap sifat fisis papan komposit beton ringan. Penelitian ini bertujuan untuk mengetahui pengaruh serat bambu terhadap sifat fisis papan komposit yang diharapkan dapat menghasilkan papan komposit beton ringan lebih kuat, lentur, ringanÂ dengan persentase serat bambu yang optimum. Penelitian dilakukan dengan menggunakan metode two dimensional reinforcement (planar) dalam penyusunan serat bambu. Persentase serat yang digunakan pada masing-masing sampel, yaitu 0%; 0,6%; 1,2%;2,5% dan 5% serta foam agent 0,06%, dan tebal sampel papan 1 cm.Â Berdasarkan hasil pengujian nilai densitas optimum diperolehÂ sebesar 1,63 g/cm3 pada serat 0,6% dari volume sampel,Â kuat lentur optimum terdapat pada serat 0,6% dari volume sampel bernilai 44,25 kg/cm2 sedangkan nilai kuat tekan optimum diperoleh sebesar 6,9 kg/cm2 pada serat 2,5% dari volume sampel. Nilai kuat lentur Â telah memenuhi standarÂ SNI 03-2104Kata kunci: Papan komposit, serat bambu, foam agent.
Analisis Pengaruh Macrobending Serat Optik Pada Sensor Glukosa Dengan Metode Evanescent
Authors: Fridayanti Nola , Muldarisnur Muldarisnur , Harmadi Harmadi 
Published: May 2018

Telah dilakukan analisis pengaruh macrobending serat optik yang telah dikupas claddingnya sepanjang 1 cm Â pada sensor glukosa dengan memanfaatkan interaksi gelombang evanescent pada serat optik dengan molekul glukosa. Cahaya dari laser dioda dengan panjang gelombang 650 nm ditransmisikan pada serat optik multimode. Pengupasan sebagian cladding serat mengakibatkan pelemahan intensitas terukur pada detektor akibat interaksi gelombang evanescent dengan molekul glukosa dalam urine. Penurunan intensitas cahaya terukur sebagai perubahan tegangan pada fotodioda yang mengindikasikan kosentrasi glukosa. Perbedaan konsentrasi glukosa berkaitan dengan indeks bias larutan glukosa (n2) yang menentukan kuat interaksi dan pelemahan gelombang evanescent. Intensitas cahaya yang sampai ke detektor meluruh secara eksponensial sebagai fungsi dari panjang pengupasan cladding serat optik. Hasil pengukuran menunjukkan bahwa tegangan keluaran dari sensor berkurang secara linear terhadap konsentrasi glukosa dengan sensitivitas yang diperoleh adalah 0,32558 (mg/dl)/mV, 0,34193 (mg/dL)/mV, dan 0,33677 (mg/dL)/mV.Â Kata kunci: macrobending, gelombang evanescent, serat optik, glukosa.
Pengaruh Variasi Ukuran Agregat Terhadap Karakteristik Beton dengan Campuran Abu Sekam Padi
Authors: Zulhijah Dilla , Handani Sri , Mulyadi Sri 
Published: May 2018

Telah dilakukan penelitian tentang pengaruh variasi ukuran agregat kasar 9,6-12,5 mm, 12,5-19 mm dan 19- 25,4 mmÂ terhadap karakteristik beton dengan campuran abu sekam padi 10 %. Semen yang digunakan yaitu Ordinary Portland Cement (OPC). Karakterisasi yang dilakukan meliputi pengujian kuat tekan, kuat tarik, porositas dan daya serap air dengan mutu beton K350. Hasil optimal untuk kuat tekan diperoleh pada ukuran agregat 9,6 â€“ 12,5 mm sebesar 487 kg/cm2, sedangkan untukÂ kuat tarik diperoleh padaÂ ukuran agregat 12,5 â€“ 19 mm sebesar 35 kg/cm2. Porositas dan daya serap air optimal diperoleh pada ukuran agregat 9,6 -12,5 mm masing - masing sebesar 1,9 %.
Rancang Bangun Sistem Telemetri Nikabel Pemantauan Tingkat Kekeruhan Air di PDAM Menggunakan Transceiver nRF24L01+ dan Arduino Uno R3
Authors: Pesma Rhahmi Adni , Harmadi Harmadi , Dahlan Dahyunir , Wildian Wildian , Syafrialdi Roni , Yuzria Hawariyi Ola , Rizky Aulia Rabiyu 
Published: May 2018

Telah dirancang sebuah sistem telemetri nirkabel pemantauan tingkat kekeruhan air di PDAM (Perusahaan Daerah Air Minum). Data tingkat kekeruhan air diambil dari air dalam reservoir PDAM yang diukur oleh sistem sensor TSD-10. Data ditransmisikan melalui udara dengan memodulasikan ke bentuk gelombang elekromagnetik oleh transceiver nRF24L01+ pada unit pemancar. Gelombang beroperasi pada frekuensi radio 2,4 GHz. Data akan diterima oleh transceiver nRF24L01+ di laboratorium PDAM pada jarak 250 m sebagai unit penerima. Hasil penelitian menunjukkan bahwa pada ruang terbuka transceiver nRF24L01+ mampu mentransmisikan data tingkat kekeruhan air saat cuaca cerah hingga 1 km dan 600 m saat cuaca hujan. Sistem sensor TSD-10 memiliki sensitivitas -1,5 mV/NTU dengan besar persentase kesalahan terbesar adalah 12,07% dan yang terkecil 0,48% serta dapat mengukur hingga 1000 NTU. Sistem ini memiliki data logging tingkat kekeruhan air pada unit penerima menggunakan program labVIEW pada komputer yang terhubung dengan arduino UNO R3. Kata kunci : telemetri nirkabel, nRF24L01+, arduino UNO R3, tingkat kekeruhan air, labVIEW.
Sintesis Dan Karakterisasi Nanopartikel Titanium Dioksida (TiO2) Menggunakan Metode Sonokimia
Authors: Astuti Astuti , Ningsi Sulastriya 
Published: May 2018

Telah dilakukan sintesis dan karakterisasi nanopartikel Titanium dioksida (TiO2) menggunakan metode sonokimia dengan larutan induk TiCl4. Sintesis dilakukan dengan variasi waktu kalsinasi di dalam tanur 1 jam, 2 jam, 3 jam, dan 6 jam. Identifikasi sifat-sifat dari TiO2 hasil sintesis melalui SEM (Scanning Electron Microscopy) dan XRD (X-ray Diffraction). Didapatkan bahwa waktu pada proses kalsinasi mempengaruhi ukuran dan morfologi partikel TiO2. Morfologi permukaan yang paling seragam terdapat pada sampel D(6 jam kalsinasi) dan kandungan anatase terbesar pada sampel B (2 jam kalsinasi). Nanopartikel TiO2 hasil sintesis tersebut berpotensi untuk diaplikasikan sebagai fotokatalis pada sel surya DSSC. Kata kunci: Nanopartikel TiO2­, kalsinasi, dan sonokimia
Fabrikasi Fiber PVA Yang Memuat Partikel TiO2 Anatase Dengan Metode Elektrospining Dan Karakteristiknya
Authors: Susiani Susiani , Harsojo Harsojo 
Published: May 2018

Fiber PVA yang memuat partikel nano TiO2 anatase telah berhasil dibuat dengan metode elektrospining. Fiber tersebut dibuat dari larutan yang merupakan campuran serbuk nano TiO2 anatase dalam 10% larutan PVA dengan rasio TiO2/PVA terbesar mencapai 60%. Proses elektrospining dilakukan pada tegangan 15 kV dengan jarak ujung anoda ke kolektor (TCD) sejauh 15 cm. Hasil analisis SEM diameter fiber dari tiap-tiap variasi rasio massa menunjukan perubahan rasio TiO2/PVA berpengaruh terhadap diameter fiber. Perlakuan pemanasan pada suhu 300pada fiber tidak mengubah fase kristalin TiO2 dan juga tidak memperlihatkan pengaruh yang signifikan terhadap diameter fiber. Namun demikian perlakuan panas tersebut dapat mereduksi ukuran partikel TiO2. Fiber yang dihasilkan mempunyai rentang diameter dari 100 -700 nm dan partikel TiO2 yang termuat pada fiber PVA memiliki rentang ukuran partikel 50 -180 nm. Hasil analisis serapan sinar UV menunjukan terjadinya serapan sinar UV pada daerah UVB, dengan demikian fiber hasil elektrospining potensial untuk digunakan sebagai fotokatalis.Kata kunci : Fiber, Polivinil Alkohol, Titanium Oksida, Elektrospining
Pengaruh Kontribusi Ketidakpastian Terhadap Pelaporan Nilai Porositas Menggunakan Metode Gravimetri
Authors: Setyawan Galih , Lestari Pratiwi Indah Tri 
Published: May 2018

Metode gravimetri berhasil diterapkan untuk menentukan nilai porositas batu kapur. Metode tersebut dilakukan dengan menggunakan timbangan standar terkalibrasi dan gelas ukur terkalibrasi untuk menentukan volume pori dan volume total dari sampel batu kapur. Porositas ditentukan dari hasil perbandingan antara volume pori dan volume total tersebut. Nilai ketidakpastian tipe A dibandingkan dengan hasil perhitungan ketidakpastian gabungan (Ugab) dengan menambahkan ketidakpastian tipe B yang bersumber dari sertifikat gelas ukur, timbangan dan temperatur. Hasilnya menunjukkan bahwa ketidakpastian tipe B menjadi penyebab utama meningkatnya nilai ketidakpastian pengukuran porositas batu kapur hingga 6%. Namun ketidakpastian tipe B seringkali diabaikan pada pelaporan hasil pengukuran.Â Kata kunci : Ketidakpastian, Porositas, Gravimetri
Effects of Clinoptilolite on Copper Accumulation of Oreochromis niloticus
Authors: ÇİFTÇİ Nuray , KARAYAKAR Fahri , CİCİK Bedii 
Published: May 2018

The copper accumulation in liver and gill tissues of Oreochromis niloticus, exposed to 2 ppm Cu
and 1g/L clinoptilolite singly and to the same concentrations of their mixture over 24, 48, 72 and
96 hours was studied. ICP-AES spectrophotometer techniques were applied in determining tissue
copper levels. Statistical evaluation of the experimental data was carried out by Student Newman
Keul’s procedure. No mortality was observed during the experiments. Copper accumulation was
lower in metal-clinoptilolite mixture group than metal singly group in gill tissue while no
accumulation in both experimental groups in liver tissue (P&lt;0.05). In addition, the copper level in
the liver was lower in all experimental groups than in the control (P&lt;0.05). Low Cu accumulation
in gill tissue exposed in mixture groups can be explained by copper adsorption with chelating
agent. The decrease of Cu reserves in the liver can be expressed by increase of copper-containing
enzyme and protein synthesis.
Analysing Sea Surface Temperature Change in Gulf of Iskenderun from 1982 to 2015
Authors: GUCEL Mehmet Ugur , SAKALLI Abdulla 
Published: May 2018

Climate change is one of the major challenge for Earth Systems in this century. Since the
Industrial revolution, energy demands of countries has been rapidly increased which also causes
an accelerated burning fossil fuels and increased greenhouse gases emission (GHG) into the
atmosphere. Sea surface temperature (SST) is a pointer of the water temperature, which mainly
related to sea surface layer and also plays an important role for keeping the energy balance
between atmosphere and marine ecosystems. Various studies show that surface temperature of
the inner seas surface temperature is affected by anthropogenic climate change. Gulf of
Iskenderun is located in the southeast Mediterranean region of Turkey. The Gulf is important
mainly for its industrial facilities and thermal power stations of Turkey. Due to the Suez Canal
and strait of Gibraltar the gulf has also very important meaning for migration of the alien species
into the Mediterranean Sea. To investigate the sea surface temperature (SST) change in the Gulf
of Iskenderun over the period of 1982-2015 we used remote sensed data, which have 4x4 km
high spatial and daily temporal resolution. This data collected from Copernicus Marine Services
which based on satellites Advanced Very High Resolution Radiometer (AVHRR) Pathfinder
Version 5.2 (PFV52). In the study period, the highest SST is determined in a JJA (June-JulyAugust)
season. The warmest region was the north is part of the bay. The SST change about 1.5º
C in the study period.
Penilaian Kinerja Dosen Menggunakan Metode TOPSIS (Studi Kasus : Amik Mitra Gama)
Authors: Surya Candra 
Published: May 2018

Untuk meningkatkan kualitas atau kinerja dosen perlu adanya penilaian. Penilaian terhadap dosen dapat dilakukan oleh Pimpinan maupun Ketua Progam Studi, seperti penilaian terhadap Pengajaran, Penelitian, Pengabdian, Tanggung Jawab, Kepribadian, Loyalitas, Kerjasama, Kepemimpinan. Permasalahan yang terjadi selama ini untuk menilai kinerja dosen masih belum optimal karena tidak adanya sebuah sistem/metode yang digunakan untuk penilaian tersebut. Oleh karena itu perlu dibuat sebuah sistem pendukung keputusan dengan metode Technique For Others Reference by Similarity to Ideal Solution (TOPSIS). Metode ini mampu memilih alternatif terbaik dari alternatif yang ada. Alternatif yang dimaksud yaitu dosen yang akan dinilai kinerjanya. Hasil yang diperoleh dengan menggunakan Metode ini adalah untuk mengetahui dosen yang memiliki kinerja terbaik. Penentuan dosen terbaik akan dikeluarkan dalam bentuk nilai angka dan diurutkan berdasarkan nilai terbesar hingga nilai yang terkecil. Dari 5 alternatif dosen yang telah diuji yaitu Dosen 1, Dosen 2, Dosen 3 Dosen 4 dan Dosen 5, maka terpilih Dosen2 yang memiliki kinerja terbaik dengan nilai preferensi 0,5341.
Penentuan Beasiswa Pada SMPN 6 Pangkalpinang Menggunakan Metode SAW dan Fuzzy Multi Attribute Decision Making
Authors: Yani Fitri , Yuranda Yuranda , Pajarini Peti , Rosmawati Rosmawati 
Published: May 2018

Beasiswa merupakan salah satu alat penting dalam memajukan pendidikan untuk siswa/siswi baik beasiswa untuk siswa berprestasi maupun beasiswa kurang mampu. Namun seringkali pihak sekolah bingung untuk menentukan siswa yang berhak menerima beasiswa tersebut, yang pada akhirnya penerima beasiswa tersebut tidak tepat sasaran.. Hal ini disebabkan karena belum adanya metode atau alat bantu yang digunakan untuk menentukan penerima beasiswa tersebut. Untuk itu dirancang suatu sistem untuk menentukan siswa yang berhak menerima beasiswa dengan menggunakan metode Simple Additive Weighting (SAW) dan Fuzzy Multi Attribute Decision Making (FMADM). Metode SAW digunakan untuk merangking dari alternatif yang ada. Metode FMADM digunakan untuk mencari alternatif dari sejumlah alternatif dengan kriteria-kriteria yang telah ditentukan. Hasil penelitian ini dapat digunakan sebagai alat bantu dalam mengambil keputusan untuk merekomendasikan sekolah dasar berbasis Islam.
Implementasi Augmanted Reality sebagai Media Pengenalan Sains Sederhana Pada Anak Usia Dini
Authors: Erna Erna 
Published: May 2018

This study aims to determine the increasing knowledge of simple science in Early Childhood in Kindergarten-right Aisyiyah Batusangkar after using Augmanted Reality technology as learning media. The type of research conducted is classroom action research. The research methodology that used in this reasearch was mixed methodology with qualitative and quantitative approach with research subject is 20 children of group B I kindegarten. The technique used in data collection and data analysis in the form of observation and subsequent interview is processed by quantitative descriptive analysis in SPSS. This classroom action research is conducted in two cycles, namely first cycle and second cycle . The introduction of children's Science in first cycle was generally still low with the average value reaches 65 %%, then second cycle plan is revised again and The increasing reaches 99% average value, positive with an increase of 34%.