bluuebunny/crossref_metadata_2025_split huggingface dataset:
The hypoxic ventilatory response is facilitated by the activation of Lkb1-AMPK signalling pathways downstream of the carotid bodies
Authors: Mahmoud Amira D. , Holmes Andrew P. , MacMillan Sandy , Ogunbayo Oluseye A. , Wyatt Christopher N. , Dallas Mark L. , Kumar Prem , Foretz Marc , Viollet Benoit , Evans A. Mark 
Published: April 2019

ABSTRACTWe recently demonstrated that the role of the AMP-activated protein kinase (AMPK), a ubiquitously expressed enzyme that governs cell-autonomous metabolic homeostasis, has been extended to system-level control of breathing and thus oxygen and energy (ATP) supply to the body. Here we assess the contribution to the hypoxic ventilatory response (HVR) of two upstream kinases that govern the activities of AMPK. Lkb1, which activates AMPK in response to metabolic stress and CaMKK2 which mediates the alternative Ca2+-dependent mechanism of AMPK activation. HVRs remained unaffected in mice with global deletion of the CaMKK2 gene. By contrast, HVRs were markedly attenuated in mice with conditional deletion of LKB1 in catecholaminergic cells, including carotid body type I cells and brainstem respiratory networks. In these mice hypoxia evoked hypoventilation, apnoea and Cheyne-Stokes-like breathing, rather than hyperventilation. Attenuation of HVRs, albeit less severe, was also conferred in mice carrying ∼90% knockdown of Lkb1 expression. Carotid body afferent input responses were retained following either ∼90% knockdown of Lkb1 or AMPK deletion. In marked contrast, LKB1 deletion virtually abolished carotid body afferent discharge during normoxia, hypoxia and hypercapnia. We conclude that Lkb1 and AMPK, but not CaMKK2, facilitate HVRs at a site downstream of the carotid bodies.
A spatiotemporal ventricular myocyte model incorporating mitochondrial calcium cycling
Authors: Song Z. , Xie L-H , Weiss J. N. , Qu Z. 
Published: April 2019

AbstractIntracellular calcium (Ca2+) cycling dynamics in cardiac myocytes are spatiotemporally generated by stochastic events arising from a spatially distributed network of coupled Ca2+ release units (CRUs) that interact with an intertwined mitochondrial network. In this study, we developed a spatiotemporal ventricular myocyte model that integrates mitochondria-related Ca2+ cycling components into our previously developed ventricular myocyte model consisting of a 3-dimensional CRU network. Mathematical formulations of mitochondrial membrane potential, mitochondrial Ca2+ cycling, mitochondrial permeability transition pore (MPTP) stochastic opening and closing, intracellular reactive oxygen species (ROS) signaling, and oxidized Ca2+/calmodulin-dependent protein kinase II (CaMKII) signaling were incorporated into the model. We then used the model to simulate the effects of mitochondrial depolarization on mitochondrial Ca2+ cycling, Ca2+ spark frequency and amplitude, which agree well with experimental data. We also simulated the effects of the strength of mitochondrial Ca2+ uniporters and their spatial localization on intracellular Ca2+ cycling properties, which substantially affected diastolic and systolic Ca2+ levels in the mitochondria but exhibited only a small effect on sarcoplasmic reticulum and cytosolic Ca2+ levels under normal conditions. We show that mitochondrial depolarization can cause Ca2+ waves and Ca2+ alternans, which agrees with previous experimental observations. We propose that this new spatiotemporal ventricular myocyte model, incorporating properties of mitochondrial Ca2+ cycling and ROS-dependent signaling, will be useful for investigating the effects of mitochondria on intracellular Ca2+ cycling and action potential dynamics in ventricular myocytes.
Comparing within- and between-family polygenic score prediction
Authors: Selzam Saskia , Ritchie Stuart J. , Pingault Jean-Baptiste , Reynolds Chandra A. , O’Reilly Paul F. , Plomin Robert 
Published: April 2019

AbstractPolygenic scores are a popular tool for prediction of complex traits. However, prediction estimates in samples of unrelated participants can include effects of population stratification, assortative mating and environmentally mediated parental genetic effects, a form of genotype-environment correlation (rGE). Comparing genome-wide polygenic score (GPS) predictions in unrelated individuals with predictions between siblings in a within-family design is a powerful approach to identify these different sources of prediction. Here, we compared within- to between-family GPS predictions of eight life outcomes (anthropometric, cognitive, personality and health) for eight corresponding GPSs. The outcomes were assessed in up to 2,366 dizygotic (DZ) twin pairs from the Twins Early Development Study from age 12 to age 21. To account for family clustering, we used mixed-effects modelling, simultaneously estimating within- and between-family effects for target- and cross-trait GPS prediction of the outcomes. There were three main findings: (1) DZ twin GPS differences predicted DZ differences in height, BMI, intelligence, educational achievement and ADHD symptoms; (2) target and cross-trait analyses indicated that GPS prediction estimates for cognitive traits (intelligence and educational achievement) were on average 60% greater between families than within families, but this was not the case for non-cognitive traits; and (3) this within- and between-family difference for cognitive traits disappeared after controlling for family socio-economic status (SES), suggesting that SES is a source of between-family prediction through rGE mechanisms. These results provide novel insights into the patterns by which rGE contributes to GPS prediction, while ruling out confounding due to population stratification and assortative mating.
Mechanistic insights into the phosphoryl transfer reaction in cyclin-dependent kinase 2: a QM/MM study
Authors: Recabarren Rodrigo , Osorio Edison H. , Caballero Julio , Tuñón Iñaki , Alzate-Morales Jans 
Published: April 2019

AbstractCyclin-dependent kinase 2 (CDK2) is an important member of the CDK family exerting its most important function in the regulation of the cell cycle. It catalyzes the transfer of the gamma phosphate group from an ATP (adenosine triphosphate) molecule to a Serine/Threonine residue of a peptide substrate. Due to the importance of this enzyme, and protein kinases in general, a detailed understanding of the reaction mechanism is desired. Thus, in this work the phosphoryl transfer reaction catalyzed by CDK2 was revisited and studied by means of hybrid quantum mechanics/molecular mechanics (QM/MM) calculations. Our results show that the base-assisted mechanism is preferred over the substrate-assisted pathway, in agreement with a previous theoretical study. The base-assisted mechanism resulted to be dissociative, with a potential energy barrier of 14.3 kcal/mol, very close to the experimental derived value. An interesting feature of the mechanism is the proton transfer from Lys129 to the phosphoryl group at the second transition state, event that could be helping in neutralizing the charge on the phosphoryl group upon the absence of a second Mg2+ ion. Furthermore, important insights into the mechanisms in terms of bond order and charge analysis were provided. These descriptors helped to characterize the synchronicity of bond forming and breaking events, and to characterize charge transfer effects. Local interactions at the active site are key to modulate the charge distribution on the phosphoryl group and therefore alter its reactivity.
Research applications of primary biodiversity databases in the digital age
Authors: Ball-Damerow Joan E. , Brenskelle Laura , Barve Narayani , Soltis Pamela S. , Sierwald Petra , Bieler Rüdiger , LaFrance Raphael , Ariño Arturo H. , Guralnick Robert 
Published: April 2019

ABSTRACTWe are in the midst of unprecedented change—climate shifts and sustained, widespread habitat degradation have led to dramatic declines in biodiversity rivaling historical extinction events. At the same time, new approaches to publishing and integrating previously disconnected data resources promise to help provide the evidence needed for more efficient and effective conservation and management. Stakeholders have invested considerable resources to contribute to online databases of species occurrences and genetic barcodes. However, estimates suggest that only 10% of biocollections are available in digital form. The biocollections community must therefore continue to promote digitization efforts, which in part requires demonstrating compelling applications of the data. Our overarching goal is therefore to determine trends in use of mobilized species occurrence data since 2010, as online systems have grown and now provide over one billion records. To do this, we characterized 501 papers that use openly accessible biodiversity databases. Our standardized tagging protocol was based on key topics of interest, including: database(s) used, taxa addressed, general uses of data, other data types linked to species occurrence data, and data quality issues addressed. We found that the most common uses of online biodiversity databases have been to estimate species distribution and richness, to outline data compilation and publication, and to assist in developing species checklists or describing new species. Only 69% of papers in our dataset addressed one or more aspects of data quality, which is low considering common errors and biases known to exist in opportunistic datasets. Globally, we find that biodiversity databases are still in the initial stages of data compilation. Novel and integrative applications are restricted to certain taxonomic groups and regions with higher numbers of quality records. Continued data digitization, publication, enhancement, and quality control efforts are necessary to make biodiversity science more efficient and relevant in our fast-changing world.
The influence of hierarchical organizational culture, supportive environment and sexual harassment on Korean women’s work-to-family enrichment
Authors: Park Sunyoung , Jo Sung Jun , Park Chan Kyun 
Published: April 2019

Purpose
The purpose of this study is to examine the relationships among hierarchical organizational culture, organizational support for women, sexual harassment and work-to-family enrichment of working women in South Korea.

Design/methodology/approach
A total of 196 responses from married female employees who had a child (or children) in for-profit organizations in South Korea were analyzed by using structural equation modeling and bootstrapping analysis.

Findings
The findings indicated that hierarchical organizational culture was negatively associated with organizational support and was positively associated with sexual harassment. In addition, an organizational atmosphere that is friendly and supportive to women had a positive effect on work-to-family enrichment. However, the more sexual harassment female workers experienced, the less positive the relationship between their work and family lives. Hierarchical organizational culture negatively affected work-to-family enrichment, but the effect was indirect through organizational support and sexual harassment.

Originality/value
These findings emphasize the importance of transforming the traditional culture in Korean organizations to reduce power distance to create a more female-friendly and supportive environment. With the dramatic recent increase in the number of female workers, building such an environment can enhance organizations’ competitiveness by creating a positive spillover effect between women’s work and family lives.
Determinants of small accommodation business size
Authors: Ye Shun , Xiao Honggen , Ying Tianyu , Zhou Lingqiang 
Published: April 2019

PurposeThis paper aims to model and empirically test the determinants of small accommodation business (SAB) size.Design/methodology/approachThis study distinguishes among three aspects of SAB size (accommodation scale, investment and employment) and between two modes of growth (managerial and entrepreneurial growth). A conceptual framework was developed based on business growth theory, whereby three econometric models were constructed and estimated to predict size variations. Data were collected through a survey on 200 SABs in North Zhejiang Province of China. Effects of the determinants were contrasted between different size indicators.FindingsThe SAB size can be affected by personal factors (employed working experience, education level, industry-specific know-how), interpersonal factor (relative ties to other SABs) and environmental factor (association support). The interpersonal and environmental factors tend to contribute to managerial growth, while the personal factors are usually conducive to entrepreneurial growth.Research limitations/implicationsThis study addresses the questions of “why some SABs grow larger in size than others” and “why different SABs grow in different ways”. But more contexts and business types should be examined so as to ensure generalizability through future studies.Practical implicationsThe research findings can provide guidelines for local tourism administration to encourage or regulate SAB development.Originality/valueThis study is among the first endeavors to examine the multidimensionality and determinants of business size in tourism and hospitality; it contributes to literature by expanding tourism entrepreneurship research into a/the “growth paradigm”.
Understanding the antecedents and consequences of the perceived usefulness of travel review websites
Authors: Wang Ping , Li Hongxiu 
Published: April 2019

Purpose
The purpose of this study is to understand the antecedents and consequences of the perceived usefulness (PU) of travel review websites.

Design/methodology/approach
An integrated model was proposed and 199 valid data were collected via a questionnaire survey from an online travel service company. The partial least squares structural equation modeling (PLS-SEM) was applied in data analysis.

Findings
The antecedents of the PU of travel review websites are utilitarian and hedonic perceptions, specifically the information quality of eWOM (utilitarian perceptions), curiosity fulfilment and enjoyment in travel review websites usage (hedonic perceptions). The PU of travel review websites positively impacts travelers’ eWOM generation, eWOM use and purchase decision.

Practical implications
This paper provides implications for travel review websites, social media managers and website designers on how to make a useful travel review website which will lead to eWOM use and generation, as well as purchase decision.

Originality/value
Prior literature has highlighted the importance of the PU of eWOM in determining travelers’ use of eWOM and purchase decision, the PU of eWOM websites has not attracted the attention of researchers. This paper is a new attempt to investigate the antecedents and consequences of the PU of travel review websites.
A review and synthesis of environmentalism within the Olympic Movement
Authors: Del Fiacco Anthony Gino , Orr Madeleine 
Published: April 2019

PurposeThe purpose of this paper is to delineate shifts in environmental sustainability leadership in the Olympic Movement through a historical narrative.Design/methodology/approachA systematic literature review was conducted and data were analyzed chronologically.FindingsFour eras are identified, distinguished by different leadership and levels of commitment toward the natural environment: public-driven environmental sustainability, host city-driven environmental sustainability, International Olympic Committee-mandated environmental sustainability and environmental regression.Research limitations/implicationsThis paper is limited to secondary data collected through a systematic literature review.Practical implicationsThe findings inform a list of best practices for Olympic environmental sustainability, grounded in evidence of past successes and lessons learned from environmentally insensitive events.Originality/valueThis is the first historical narrative and synthesis of environmental leadership in the Olympic Movement, a topic previously covered in studies focused solely on the institutions responsible for hosting the event, ignoring external parties and the deep history of environmentalism dating back to the 1930s, or focusing just on actions, ignoring the actors who drove the environmental movement.
Rediscovering relevance
Authors: Lambert Douglas M. 
Published: April 2019

PurposeThe purpose of this paper is to document the growing concerns about the lack of relevance of business school research, and offer suggestions for journal editors and faculty members in logistics and supply chain management.Design/methodology/approachThis paper is a review of literature related to the relevance of business school research and an analysis of trends related to the editorial review boards of the three main logistics journals.FindingsThe current business school research model is unsustainable. The metrics used are driving the wrong behavior. Logistics journals, traditionally known for an emphasis on practical relevance, appear to be emulating the journals of other business functions at a time when there is a growing consensus that these journals are publishing, more often than not, research of little or no value to practicing managers or society.Practical implicationsThe cost of faculty research at AACSB schools, which increasingly benefits no one but the authors, has been estimated at roughly US$3.8bn annually. Imagine the potential benefits if business school deans realigned the incentives to encourage faculty to produce credible research that is useful to business and society.Originality/valueThe hope is to influence senior logistics faculty with tenure to work with business executives or policymakers to identify long-term big idea projects that will impact business and society, and publish their research in the logistics journals. Traditionally, the editors of logistics journals included business executives on the editorial review boards and encouraged research of practical relevance. Journal editors should look back and realize what was good about the journals and not discard the good for current fads.
After all these years, what has happened to the international prevalence of NPM-inspired managerial practices?
Authors: Aoki Naomi 
Published: April 2019

Purpose
According to a widely accepted narrative, managerial reforms associated with new public management (NPM) originated in wealthy market economies and liberal democracies and were then promoted globally. However, scientific and systematic cross-national evidence of NPM practices has remained limited in scope, and debates over their survival and prevalence remain unsettled. The purpose of this paper is to narrow this empirical gap.

Design/methodology/approach
Using international data from public education in approximately 65 economies in 2012, this study systematically investigated the prevalence of managerial practices, namely, the managerial responsibilities of school principals, goal orientation in school management, and performance-based human resource management (HRM). It also tested correlations between the status of these practices and political and economic conditions across economies.

Findings
As of 2012, the top users of NPM practices were geographically spread across the world in regions that included Southeast Asia, Eastern Europe, the Middle East, and North Africa. Economies exhibited dissimilarities in their deployment of managerial practices. Performance-based HRM tended to be used more extensively in less accountable and less wealthy economies.

Originality/value
By focusing on actual practices, this study offers an empirically valid, critical analysis of the global prevalence of NPM. The findings clarify some observers’ current understandings of NPM. They deliver a powerful message that debates on global reform waves can benefit greatly from empirical evidence drawn from world regions beyond one’s parochial focus.
Historical policies affecting women’s military and family roles
Authors: King Erika Lee , DiNitto Diana M. 
Published: April 2019

Purpose
The US military depends on women to meet recruiting goals, but women participate at lower rates than men. Theorists suggest that military and family policies affect women’s lower participation. Research has confirmed the impact of policy changes on women’s military service during specific time periods. The purpose of this paper is to examine how and when military policies affecting women developed over the course of history, exploring two related hypotheses: first, when women’s military participation is vital, policies affecting their military and family roles punctuate in tandem, and second, cultural values impact policy solutions to reconcile women’s roles.

Design/methodology/approach
Punctuated equilibrium and a women’s military participation theory informed the hypotheses. US Census and Defense Department data were used to identify periods of service when women’s military participation was vital. Historical policies were mapped and analyzed to identify policy patterns and themes affecting women’s military participation 1895–2015.

Findings
Evidence supports both hypotheses. When women are needed during wartimes, policies simultaneously encourage their service and regulate their family roles. However, policies evolved from separating servicewomen’s roles prior to the 1970s (e.g. prohibiting motherhood), to supporting their families (e.g. maternity leave) – a shift precipitated by sweeping changes in broader society and the military’s change from the draft to an All-Volunteer Force.

Originality/value
Findings elucidate the link between military and family policies affecting US women’s military participation and retention. Results may inform policy advocacy aimed at optimizing the US Department of Defense’s diversity efforts.
Modelling wholesale distribution operations: an artificial intelligence framework
Authors: Bottani Eleonora , Centobelli Piera , Gallo Mosé , Kaviani Mohamad Amin , Jain Vipul , Murino Teresa 
Published: April 2019

Purpose
The purpose of this paper is to propose an artificial intelligence-based framework to support decision making in wholesale distribution, with the aim to limit wholesaler out-of-stocks (OOSs) by jointly formulating price policies and forecasting retailer’s demand.

Design/methodology/approach
The framework is based on the cascade implementation of two artificial neural networks (ANNs) connected in series. The first ANN is used to derive the selling price of the products offered by the wholesaler. This represents one of the inputs of the second ANN that is used to anticipate the retailer’s demand. Both the ANNs make use of several other input parameters and are trained and tested on a real wholesale supply chain.

Findings
The application of the ANN framework to a real wholesale supply chain shows that the proposed methodology has the potential to decrease economic loss due to OOS occurrence by more than 56 percent.

Originality/value
The combined use of ANNs is a novelty in supply chain operation management. Moreover, this approach provides wholesalers with an effective tool to issue purchase orders according to more dependable demand forecasts.