bluuebunny/crossref_metadata_2025_split huggingface dataset:
The 3D imaging of mesenchymal stem cells on porous scaffolds using high‐contrasted x‐ray computed nanotomography
Authors: VOJTOVÁ L. , ZIKMUND T. , PAVLIŇÁKOVÁ V. , ŠALPLACHTA J. , KALASOVÁ D. , PROSECKÁ E. , BRTNÍKOVÁ J. , ŽÍDEK J. , PAVLIŇÁK D. , KAISER J. 
Published: November 2018

SummaryThis study presents an X‐ray computed nanotomography (nano‐CT) based, high‐resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab‐based nano‐CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano‐CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X‐ray analysis (EDX) established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three‐dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.Lay DescriptionBiomaterials play a crucial role in tissue engineering by serving as 3D scaffolds for cellular attachment, proliferation, and in growth ultimately leading to new tissue formation. Cell morphology and proliferation inside the 3D scaffold are necessary to know for assessing cell viability. However, these studies are usually negatively affected by the limitations of imaging techniques. We demonstrate that X‐ray computed nanotomography (nano‐CT), based on high‐resolution imaging technique providing voxel resolution of 540 nm, is a suitable method for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using a lab‐based nano‐CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano‐CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy in a combination with energy dispersive X‐ray analysis established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three‐dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.
Resuscitative hysterotomy in a patient with peripartum cardiomyopathy
Authors: Kamei Hidetake , Wakimoto Yu , Harada Kayoko , Fukui Atsushi , Tanaka Hiroyuki , Shibahara Hiroaki 
Published: November 2018

AbstractResuscitative hysterotomy (RH) is a resuscitation technique, allowing the restoration of a pregnant patient's heartbeat. Here, we reported a case of RH performed in a patient with cardiac arrest as a complication of a peripartum cardiomyopathy. A 29‐year‐old woman with suspected hemolysis, elevated liver enzymes, low platelet syndrome was admitted to the hospital. Cardiopulmonary resuscitation and RH were initiated at 30 weeks of gestation. The infant was successfully delivered 2 min after the mother's cardiac arrest, weighting 1388 g. At the first minute, the Apgar score was 3 and the 5th minute was 6. After delivery, defibrillation was performed on the mother and restoration of spontaneous circulation was observed. However, she was hemodynamically unstable and approximately 2 months later she died. After cardiac arrest, it is possible that RH could improve the hemodynamic status. The opportunity of performing a RH is rare; however, it is necessary to be familiarized with the technique as a resuscitation method.
The population genomics of multiple tsetse fly (Glossina fuscipes fuscipes) admixture zones in Uganda
Authors: Saarman Norah P. , Opiro Robert , Hyseni Chaz , Echodu Richard , Opiyo Elizabeth A. , Dion Kirstin , Johnson Thomas , Aksoy Serap , Caccone Adalgisa 
Published: November 2018

AbstractUnderstanding the mechanisms that enforce, maintain or reverse the process of speciation is an important challenge in evolutionary biology. This study investigates the patterns of divergence and discusses the processes that form and maintain divergent lineages of the tsetse flyGlossina fuscipes fuscipesin Uganda. We sampled 251 flies from 18 sites spanning known genetic lineages and the four admixture zones between them. We apply population genomics, hybrid zone and approximate Bayesian computation to the analysis of three types of genetic markers: 55,267 double‐digest restriction site‐associated DNA (ddRAD) SNPs to assess genome‐wide admixture, 16 microsatellites to provide continuity with published data and accurate biogeographic modelling, and a 491‐bp fragment of mitochondrial cytochrome oxidase I and II to infer maternal inheritance patterns. Admixture zones correspond with regions impacted by the reorganization of Uganda's river networks that occurred during the formation of the West African Rift system over the last several hundred thousand years. Because tsetse fly population distributions are defined by rivers, admixture zones likely represent both old and new regions of secondary contact. Our results indicate that older hybrid zones contain mostly parental types, while younger zones contain variable hybrid types resulting from multiple generations of interbreeding. These findings suggest that reproductive barriers are nearly complete in the older admixture zones, while nearly absent in the younger admixture zones. Findings are consistent with predictions of hybrid zone theory: Populations in zones of secondary contact transition rapidly from early to late stages of speciation or collapse all together.
Spatial and temporal changes in species composition of submersed aquatic vegetation reveal effects of river restoration
Authors: Carhart Alicia M. , De Jager Nathan R. 
Published: November 2018

We examined temporal changes in spatial patterns of submersed aquatic vegetation (SAV) in response to the restoration of geomorphic habitat in Navigation Pool 8 of the Upper Mississippi River from 1998 to 2016. The frequency of occurrence and species composition of SAV at sampling sites were spatially interpolated for each year to create annual maps. Linear models were fitted to temporal changes in SAV within each map pixel. The frequency of occurrence of SAV (across all species) increased over time in much of the impounded region of the pool, including areas near restored islands. However, impounded areas maintained a relatively consistent species composition over time, with species known to be tolerant of higher flow velocities (>0.10 m/second) and wind fetch distances (>1,000 m) (e.g. Vallisneria americana) being most abundant. In contrast, areas protected by newly constructed islands transitioned from V. americana to species found in other protected backwater habitats and known to be intolerant of high flow velocities and wind fetch distances (e.g. Ceratophyllum demersum). The results suggest that previously reported improvements in water clarity may have improved growing conditions for all SAV species, especially in the lower impounded portion of the pool, while island restoration created more backwater‐like habitats and facilitated changes in species composition. Assessing changes in SAV occurrence alone offers only a partial view of local‐scale river restoration (e.g. island building), while analyses of species composition are likely to be more indicative of the types of changes (i.e. reduced flow velocity and wind fetch) associated with restoring geomorphic habitat.
Role of arginine deiminase in thymic atrophy during experimental Streptococcus pyogenes infection
Authors: Starikova Eleonora Alexandrovna , Golovin Alexander Stanislavovich , Vasilyev Kirill Alexandrovich , Karaseva Alena Borisovna , Serebriakova Maria Konstantinovna , Sokolov Alexey Victorovich , Kudryavtsev Igor Vladimirovich , Burova Larissa Alexandrovna , Voynova Irina Vitalyevna , Suvorov Alexander Nikolaevich , Vasilyev Vadim Borisovich , Freidlin Irina Solomonovna 
Published: November 2018

AbstractExpression of gene of arginine deiminase (AD) allows adaptation of Streptococcus pyogenes to adverse environmental conditions. AD activity can lead to L‐arginine deficiency in the host cells’ microenvironment. Bioavailability of L‐arginine is an important factor regulating the functions of the immune cells in mammals. By introducing a mutation into S pyogenes M46‐16, we obtained a strain with inactivated arcA/sagp gene (M49‐16 delArcA), deficient in AD. This allowed elucidating the function of AD in pathogenesis of streptococcal infection. The virulence of the parental and mutant strains was examined in a murine model of subcutaneous streptococcal infection. L‐arginine concentration in the plasma of mice infected with S pyogenes M49‐16 delArcA remained unchanged in course of the entire experiment. At the same time mice infected with S pyogenes M49‐16 demonstrated gradual diminution of L‐arginine concentration in the blood plasma, which might be due to the activity of streptococcal AD. Mice infected with S pyogenes M49‐16 delArcA demonstrated less intensive bacterial growth in the primary foci and less pronounced bacterial dissemination as compared with animals infected with the parental strain S pyogenes M46‐16. Similarly, thymus involution, alterations in apoptosis, thymocyte subsets and Treg cells differentiation were less pronounced in mice infected with S pyogenes M49‐16 delArcA than in those infected with the parental strain. The results obtained showed that S pyogenes M49‐16 delArcA, unable to produce AD, had reduced virulence in comparison with the parental S pyogenes M49‐16 strain. AD is an important factor for the realization of the pathogenic potential of streptococci.
Discrepancy between true distance of tibial tuberosity advancement and cage size: An ex vivo study
Authors: Jin Dong‐Woo , Peck Jeffrey N. , Tano Cheryl A. , Morgan Matthew J. 
Published: November 2018

AbstractObjectiveTo determine the discrepancy between true distance of tibial tuberosity advancement (tTTA) and the most commonly used cages. The null hypothesis was that tTTA would be within 1.5 mm difference of the cage size.Study designEx vivo study.Sample populationEight foam cortical shell specimens modeled from canine tibias.MethodsModified tibial tuberosity advancement (TTA) was performed to allow repeated measurement of the tTTA. The Pearson correlation test was used to evaluate the relationships of tTTA and cage sizes (P < .05). The difference between tTTA and cage size was calculated to establish the degree of underadvancement during the TTA.ResultsTwo hundred forty tTTA measurements were recorded. The tTTA was less than each corresponding cage size (P < .001). Four cage sizes resulted in a difference greater than 1.5 mm (P < .001‐.04). The 6‐mm cage resulted in median tTTA of 4.3 mm, which did not differ significantly from 4.5 mm (range, 3.2‐6.65). The median underadvancement ranged from 1.7 to 2.5 mm for the tested cage sizes. The percentage underadvancement ranged from 21% to 28% for the tested cage sizes.ConclusionThe tTTA was less than the corresponding cage sizes by at least 1.5 mm in all except the 6‐mm cage.Clinical significanceSelection of a larger cage size during the TTA may be advantageous to compensate for underadvancement and to minimize the risk of residual cranial tibial translation.
Intraocular lens dislocation: a novel in-situ scleral refixation technique using a 25 Gauge trocar in the anterior chamber
Authors: Giansanti Fabrizio , Tartaro Ruggero , Caporossi Tomaso , Murro Vittoria , Savastano Alfonso , Barca Francesco , Bacherini Daniela , Fiore Tito , Cagini Carlo , Rizzo Stanislao 
Published: November 2018

ObjectiveIntraocular lens (IOL) repositioning using a closed-eye approach could be carried out in some selected cases. Our study focuses on the efficacy and safety of a IOL closed-eye repositioning technique using scleral suture, which is performed using a trocar as an intrastromal limbal guide.Methods and analysisThirty-one eyes of 31 patients with late IOL dislocation operated on between January 2015 and May 2017 were included in this retrospective non-comparative consecutive case series study. The patients had a single-piece in-the-bag dislocation or a 3-pieces in-the-bag or out-of-the-bag dislocation. The patients underwent an anterior vitrectomy and a scleral refixation in a closed chamber using a 10/0 polypropylene suture passed through a 25 Gauge trocar inserted in the anterior chamber.ResultsThe mean follow-up time was 19.54 months. Average preoperative best-corrected visual acuity (BCVA) was 0.73 LogMar (±0.21 SD); while average postoperative BCVA was 0.27 LogMar (±0.23 SD). Fifteen patients underwent anterior pars plana vitrectomy (PPV) while 16 patients did not; moreover, two patients underwent PPV. Six patients had an increase of postoperative intraocular pressure, two patients had postoperative decentration, two patients had postoperative cystoid macular oedema, none of the patients had major complications such as retinal detachment, choroidal detachment, malignant glaucoma, irreversible corneal decompensation and endophthalmitis.ConclusionWe can affirm that our technique may be safe and useful in the case of 3-piece in-the bag or out-of the bag dislocated IOLs and also in the case of in-the-bag single-piece dislocated IOLs.
Palliative care in a tertiary neonatal intensive care unit: a 10-year review
Authors: Ng Stanley Ka Fai , Keenan Ngaire , Swart Sophie , Berry Mary Judith 
Published: November 2018

ObjectivesWhen active treatment is no longer in the best interests of the patient, redirection of care to palliation is an important transition. We review, within a tertiary neonatal intensive care unit (NICU), the journey leading to the decision to redirect care, the means of symptom control and the provision of psychosocial supports.MethodsA retrospective review of all 166 deaths of NICU-affiliated patients during a 10- year epoch. Medical notes were reviewed, and the provision and type of, or barriers to, effective palliative care was defined.ResultsExtreme prematurity accounted for 71/145 (49%) of deaths with relatively high proportions of Māori 17/71 (25%) and Pacific Islanders 9/71 (13%). Almost all eligible infants received some form of palliation. Transition from curative to palliative care was refused by the family in a single case. Median time from decision to redirect care until first recorded action was 80 min, and median time from action until death was 60 min. The majority of infants received some form of comfort cares, (128/166) most commonly morphine (94/128, 73%). Three infants had documented seizure activity or respiratory distress but did not receive any pharmacological intervention. Psychosocial supports were offered in 98/145 (67%) of cases, but only 71/145 (49%) of families were formally offered an opportunity to discuss the infant’s clinical course after their death.ConclusionsClinical documentation of care plans was often incomplete, potentially leading to inconsistent delivery of care, increased risk of symptom breakthrough and/or inadequate psychosocial supports for family. Formal individualised palliative care plans are under development to standardise documentation and improve therapeutic and psychosocial interventions available to the infant and their family.
The effectiveness of a couple-based intervention for people living with mild Alzheimer’s disease and their spousal caregivers in Korea
Authors: Ha Jung-Hwa , Kwak Minyoung , Han Ji Won , Lee Hyeon Jung , Ingersoll-Dayton Berit , Spencer Beth , Kim Ki Woong 
Published: November 2018

Background and objectives We evaluated the effectiveness of a Korean version of the Couples Life Story Approach (CLSA-K), a structured life-review program originally developed in the U.S. to help older adults with dementia and their spousal caregivers engage with each other and improve their quality of life. Research design and methods: Fifty mild people living with mild Alzheimer’s disease and their spouses were recruited and 37 couples completed the five-week CLSA-K program. Data on psychosocial outcomes—i.e., depressive symptoms, talkativeness, mutuality (for both caregivers and care recipients) and burden (for caregivers)—were collected one week prior to (Time 1) and one week after the intervention (Time 2). Using repeated measures generalized linear models, we examined the differences in the amounts and patterns of the changes in outcomes according to age, gender, and the care-recipients’ level of cognitive impairment. Results Some participants benefited from the CLSA-K program, while others did not. For caregivers, mental health, and marital quality remained stable, while caregiver burden increased among those with spouses who had moderate levels of cognitive impairment. For care-recipients, younger and male participants showed increased talkativeness. Discussion and implications CLSA-K appears to be promising for specific subgroups of participants in Korea. Multi-component or other approaches may be more beneficial for other couples. Cultural differences as well as social positions may play a role in the acceptability and efficacy of the couple-based intervention.
Infrarenal Aortic Endograft Infection: A Single-Center Experience
Authors: Fernandez Prendes Carlota , Riedemann Wistuba Mariel , Zanabili Al-Sibbai Ahmad Amer , Del Castro Madrazo Jose Antonio , Santervas Lino Antonio Camblor , Perez Manuel Alonso 
Published: November 2018

Purpose: Endograft infection is an infrequent but one of the most serious and challenging complications after endovascular aortic repair. The aim of this study was to assess the management of this complication in a tertiary center. Case Series: A retrospective analysis of a prospective database was performed including all patients who underwent elective endovascular abdominal aortic repair (EVAR) from 2003 to 2016 in a tertiary center. Seven cases of endograft infection were identified during the follow-up period from a total of 473 (1.48%) EVAR. Most frequent symptoms at presentation were fever (71.4%) and lumbar pain (57.1%). One case developed an early infection, while 6 cases were diagnosed as late infections. Mean time from endograft placement to symptom presentation was 28.3 months (2-91.5 months). Gram-positive cocci were the microorganisms most commonly isolated in blood cultures (66%). Two cases were managed with endograft removal and aortic reconstruction with a cryopreserved allograft, 2 cases with surgical drainage, and 2 cases exclusively with antibiotic therapy. In 1 case, the diagnosis was performed postoperatively based on intraoperative findings associated with positive graft cultures; and graft explantation was performed with “in situ” reconstruction using a Dacron graft. Perioperative mortality was 42.9%. One-year mortality was 57.1%. Mean follow-up was 21.5 months. Conclusion: Endograft explantation is the gold standard of treatment; however, given the overall high morbi–mortality rates of this pathology, a tailored approach should always be offered depending on the patient’s overall condition. Conservative management can be an acceptable option in those patients with short life expectancy and high surgical risk.
The influence of tourist evaluative factors on tourist behavioral intention: the mediating role of tourist satisfaction
Authors: Fernaldi Elmer Hafiizh , Sukresna I Made 
Published: November 2018

This study examines the effect of tourist evaluative factors, specifically service quality and tourist experience on tourist satisfaction and its further influence on revisit intention and intention to recommend. Service quality and tourist experience serve as independent variables, while revisit intention and recommend intention are posited as dependent variables, with satisfaction acts as the intervening variable. Data analysis using Structural Equation Modeling (SEM) of 186 tourist visiting Umbul Sidomukti, Semarang, Central Java, Indonesia, finds that both revisit intention and intention to recommend could be increased through the improvement of service quality and tourist satisfaction.
Tangential derivatives and higher-order regularizing properties of the double layer heat potential
Authors: Lanza de Cristoforis Massimo , Luzzini Paolo 
Published: November 2018

Abstract
We prove an explicit formula for the tangential derivatives of the double layer heat potential.
By exploiting such a formula, we prove the validity of a regularizing property for the integral operator associated to the double layer heat potential in spaces of functions with high-order derivatives in parabolic Hölder spaces defined on the boundary of parabolic cylinders which are unbounded in the time variable.
La peur, passion radicale
Authors: Morel Pierre-Marie 
Published: November 2018

AbstractThis paper deals with the aetiological aspect of the Epicurean conception of passions. It is argued that the therapy of the soul relies on an overall explanation of the various emotional states, and that fear is at the core of this framework in so far as it is the root of most psychic troubles. The first part deals with fear in the context of the general theory of passions. The second part shows that this basic emotion is the root of injustice and political excessive ambition, in Lucretius’De rerum natura. The third part puts the emphasis on the role of anxiety in passionate love, in the same text. Fear, in its various forms, appears to be the main cause of a number of pathological emotions. It may be concluded that the Epicurean therapy of emotions, far from dealing with separate passions from case to case, is based on a holistic approach of psychic states.
La trattazione vetero-stoica dell’ira: componenti logiche e caratteri fisiologici
Authors: Alesse Francesca 
Published: November 2018

AbstractThe paper compares the two components of the Stoic definition ofpathos, the logico-linguistic component and the physiological, ‘pneumatic’ one, in order to examine the role that each of them plays in the onset ofpathos, its persistence in the human soul, its possible eradication. The analysis is focused on anger (ὀργή) which reveals some peculiarities, especially as regards its logical structure, due to the fact that this passion seems to be the combination of two different feelings. The anger indeed is classified as a species of desire but the description of its onset also includes pain.