bluuebunny/crossref_metadata_2025_split huggingface dataset:
SWATH‐based proteomics reveals processes associated with immune evasion and metastasis in poor prognosis colorectal tumours
Authors: López‐Sánchez Laura M. , Jiménez‐Izquierdo Rafael , Peñarando Jon , Mena Rafael , Guil‐Luna Silvia , Toledano Marta , Conde Francisco , Villar Carlos , Díaz César , Ortea Ignacio , De la Haba‐Rodríguez Juan R. , Aranda Enrique , Rodríguez‐Ariza Antonio 
Published: September 2019

AbstractNewly emerged proteomic methodologies, particularly data‐independent acquisition (DIA) analysis–related approaches, would improve current gene expression–based classifications of colorectal cancer (CRC). Therefore, this study was aimed to identify protein expression signatures using SWATH‐MS DIA and targeted data extraction, to aid in the classification of molecular subtypes of CRC and advance in the diagnosis and development of new drugs. For this purpose, 40 human CRC samples and 7 samples of healthy tissue were subjected to proteomic and bioinformatic analysis. The proteomic analysis identified three different molecular CRC subtypes: P1, P2 and P3. Significantly, P3 subtype showed high agreement with the mesenchymal/stem‐like subtype defined by gene expression signatures and characterized by poor prognosis and survival. The P3 subtype was characterized by decreased expression of ribosomal proteins, the spliceosome, and histone deacetylase 2, as well as increased expression of osteopontin, SERPINA 1 and SERPINA 3, and proteins involved in wound healing, acute inflammation and complement pathway. This was also confirmed by immunodetection and gene expression analyses. Our results show that these tumours are characterized by altered expression of proteins involved in biological processes associated with immune evasion and metastasis, suggesting new therapeutic options in the treatment of this aggressive type of CRC.
Variations of Wnt/β‐catenin pathway‐related genes in susceptibility to knee osteoarthritis: A three‐centre case‐control study
Authors: Huang Yong , Jiang Lifeng , Yang Haoyu , Wu Lidong , Xu Nanwei , Zhou Xindie , Li Jin 
Published: September 2019

AbstractExcessive activation of the Wnt signalling pathway in the articular cartilage is demonstrated to be related to the onset and severity of osteoarthritis (OA). However, few studies have investigated the association between variants in Wnt‐pathway‐related genes and the risk of OA by searching Pubmed and EMBASE. Totally, 471 knee OA patients and 532 controls were recruited from three hospitals to evaluate the associations of five genetic variants (rs61735963, rs2908004, rs10795550, rs1799986 and rs1127379) with the risk of knee OA. These polymorphisms were genotyped through polymerase chain reaction and Sanger sequencing. Genetic risk scores (GRSs) were calculated to evaluate the combined effect of these genetic variants. No significant association was found between OA risk and polymorphisms (rs61735963, rs10795550 or rs1127379). However, WNT16 rs2908004 polymorphism was correlated with a decreased risk of OA, especially among females, smokers, non‐drinkers and individuals with age < 60 years or BMI ≥ 25. This SNP was also associated with Kellgren‐Lawrence grade and CRP. Similarly, LRP1 rs1799986 polymorphism decreased the risk of OA among males, smokers, drinkers and individuals with age < 60 years or BMI ≥ 25. TT genotype was more frequent in the group of VAS ≥ 6 versus VAS < 6. A low GRS was positively correlated with a decreased risk of OA. In addition, rs2908004 or rs1799986 polymorphism reduces the expression of WNT16 or LRP1. In conclusion, two SNPs (rs2908004 and rs1799986) are associated with the decreased risk of OA by regulating the Wnt pathway.
Early sensory neurophysiological changes in prediabetes
Authors: Lin Yi‐Chen , Lin Cindy Shin‐Yi , Chang Tsui‐San , Lee Jing‐Er , Tani Jowy , Chen Hung‐Ju , Sung Jia‐Ying 
Published: September 2019

AbstractAims/IntroductionTo elucidate whether axonal changes arise in the prediabetic state and to find a biomarker for early detection of neurophysiological changes.Materials and MethodsWe enrolled asymptomatic diabetes patients, as well as prediabetic and normoglycemic individuals to test sensory nerve excitability, and we analyzed those findings and their correlation with clinical profiles.ResultsIn nerve excitability tests, superexcitability in the recovery cycle showed increasing changes in the normoglycemic, prediabetes and diabetes cohorts (−19.09 ± 4.56% in normoglycemia, −22.39 ± 3.16% in prediabetes and −23.71 ± 5.15% in diabetes, P = 0.002). Relatively prolonged distal sensory latency was observed in the median nerve (3.12 ± 0.29 ms in normoglycemia, 3.23 ± 0.38 ms in prediabetes and 3.45 ± 0.43 ms in diabetes, P = 0.019). Superexcitability was positively correlated with fasting plasma glucose (r = 0.291, P = 0.009) and glycated hemoglobin (r = 0.331, P = 0.003) in all participants.ConclusionsSensory superexcitability and latencies are the most sensitive parameters for detecting preclinical physiological dysfunction in prediabetes. In addition, changes in favor of superexcitability were positively correlated with glycated hemoglobin for all participants. These results suggest that early axonal changes start in the prediabetic stage, and that the monitoring strategy for polyneuropathy should start as early as prediabetes.
Concordance as a Person‐Centered Measure of Breastfeeding Success: From Adequacy to Agency
Authors: Eagen‐Torkko Meghan 
Published: October 2019

AbstractHealth benefits associated with breastfeeding are supported by extensive research. Measurements of breastfeeding success in clinical and research settings have focused on maximizing adherence to population‐level health recommendations for infant feeding (compliance). Concordance, a new adjunct measure of breastfeeding success, uses a comparison between the parent's intended feeding method and the actual feeding method to better assess whether the person's goals for infant feeding have been met. This measure uses a modified patient‐centered outcomes research question to evaluate success in a person‐centered framework and is congruent with patient‐centered individualized care and shared decision making. Use of concordance as an adjunct success metric in evaluation of interventions and clinical programs will provide an opportunity to approach breastfeeding promotion holistically and incorporate the parent's desired outcome(s) as central to that success.
Targeting aurora kinase B alleviates spinal microgliosis and neuropathic pain in a rat model of peripheral nerve injury
Authors: Shen Yu , Ding Zhuofeng , Ma Shengyun , Zou Yu , Yang Xin , Ding Zijin , Zhang Yu , Zhu Xiaoyan , Schäfer Michael K. E. , Guo Qulian , Huang Changsheng 
Published: September 2019

AbstractPeripheral nerve injury elicits spinal microgliosis, contributing to neuropathic pain. The aurora kinases A (AURKA), B (AURKB), and C (AURKC) are potential therapeutic targets in proliferating cells. However, their role has not been clarified in microglia. The aim of this study was to examine the regulation of aurora kinases and their roles and druggability in spinal microgliosis and neuropathic pain. Sprague–Dawley rats received chronic constriction injury (CCI). Gene expression of aurora kinases A‐C was evaluated by quantitative RT‐PCR and western blot, respectively, in spinal cords at 1, 3, 7, and 14 days after CCI. AURKB gene and protein expression was up‐regulated concomitantly with the development of spinal microgliosis and neuropathic pain. Using lentiviral over‐expression and adeno‐associated viral knockdown approaches, the function of AURKB was further investigated by western blot, immunohistochemistry, RNA sequencing, and pain behavior tests. We found that AURKB over‐expression in naive rats caused spinal microgliosis and pain hypersensitivity, whereas AURKB knockdown reduced microgliosis and alleviated CCI‐induced neuropathic pain. Accordingly, RNA sequencing data revealed down‐regulation of genes critically involved in signaling pathways associated with spinal microgliosis and neuropathic pain after AURKB knockdown in CCI rats. To examine its therapeutic potential for treatment of neuropathic pain, animals were treated intrathecally with the pharmacological AURKB inhibitor AZD1152‐HQPA resulting in the alleviation of CCI‐induced pain. Taken together, our findings indicated that AURKB plays a critical role in spinal microgliosis and neuropathic pain. Targeting AURKB may be an efficient method for treatment of neuropathic pain subsequent to peripheral nerve injury.
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Individuals at risk of seropositive rheumatoid arthritis: the evolving story
Authors: Rantapää Dahlqvist S. , Andrade F. 
Published: September 2019

AbstractThe aetiology of the autoimmune disease rheumatoid arthritis (RA) involves a complex interplay between genetic and environmental factors that initiate many years before the onset of clinical symptoms. These interactions likely include both protective and susceptibility factors which together determine the risk of developing RA. More than 100 susceptibility loci have been linked to RA. The strongest association is with HLA‐DRB1 alleles encoding antigen presenting molecules containing a unique sequence in the peptide‐binding grove called the ‘shared epitope’. Female sex, infections during childhood, lifestyle habits (e.g. smoking and diet) and distinct microbial agents, amongst many others, are interacting risk factors thought to contribute to RA pathogenesis by dysregulating the immune system in individuals with genetic susceptibility. Interestingly, patients with RA develop autoantibodies many years before the clinical onset of disease, providing strong evidence that the lack of tolerance to arthritogenic antigens is amongst the earliest events in the initiation of seropositive RA. Here, we will discuss the clinical and mechanistic evidence surrounding the role of different environmental and genetic factors in the phases leading to the production of autoantibodies and the initiation of symptomatic RA. Understanding this complexity is critical in order to develop tools to identify drivers of disease initiation and propagation and to develop preventive therapeutics.
The reciprocal relationship between affective organizational commitment and role overload: When autonomy need satisfaction meets the individual self‐concept
Authors: Tang Wei‐Gang , Vandenberghe Christian 
Published: September 2019

This study examines the mediating role of autonomy need satisfaction in the reciprocal relationship between affective organizational commitment (AOC) and role overload over time, and the moderating role of the individual self‐concept. Using panel data collected at two points in time from 263 employees working for different organizations across various industries in China, we found that autonomy need satisfaction mediated a positive relationship from AOC to role overload over time. In contrast, the reciprocal sequential relationship from role overload to AOC through autonomy need satisfaction was non‐significant. Finally, the employees’ individual self‐concept acted as a moderator such that the relationship from autonomy need satisfaction to role overload over time was stronger (vs. weaker) when employees’ individual self‐concept was high (vs. low). We discuss the implications of these findings for future research on employee commitment, autonomy need satisfaction, and role overload.Practitioner points

Autonomy need satisfaction was related to increased role overload over time.

Affective organizational commitment was indirectly related to increased role overload through autonomy need satisfaction.

The relationship from autonomy need satisfaction to role overload was stronger when the individual self‐concept was high.

Employees’ sense of individuation is a central aspect of how role overload develops.
An outbreak of multiple genotypes of Listeria monocytogenes in New Zealand linked to contaminated ready-to-eat meats—a retrospective analysis using whole-genome sequencing
Authors: Rivas L. , Dupont P.-Y. , Wilson M. , Rohleder M. , Gilpin B. 
Published: September 2019

Abstract
Four cases of listeriosis in a hospital (A) in New Zealand were identified in 2012. Pulsed-field gel electrophoresis (PFGE) used at the time identified four pulsotypes amongst the clinical isolates. Two of the pulsotypes matched to Listeria monocytogenes isolates obtained from ready-to-eat (RTE) meat samples from a RTE producer tested during a nationwide microbiological survey the month prior. The outbreak investigation confirmed that the RTE producer had supplied product to the hospital and additional testing confirmed the presence of L. monocytogenes in RTE meats from the hospital kitchen. Two further listeriosis cases presented in another hospital (B) with one clinical isolate identified as the same pulsotype as identified for one case in hospital A, but the epidemiology information concluded that the clinical cases from hospital B were not linked to the outbreak. Retrospective whole-genome sequencing confirmed that epidemiologically linked isolates belonging to three different genotypes for clinical cases from hospital A and RTE meats samples from the hospital kitchen differed by 0-1 core-genome locus or single nucleotide polymorphisms (SNP). The use of core-genome multilocus sequence typing and SNP analysis provided a greater degree of discrimination between isolates compared to PFGE.

Significance and Impact of the Study
This study describes a listeriosis outbreak associated with a hospital in New Zealand and attributed to contaminated ready-to-eat (RTE) meat supplied to the hospital by a single producer. Retrospective whole-genome sequence analysis of outbreak isolates was found to provide a greater degree of discrimination between isolates compared to pulsed-field gel electrophoresis and supported the conclusions made at the time of the outbreak. The multiple genotypes identified from clinical cases and the RTE meats obtained during the outbreak highlight the importance of epidemiological concordance alongside genotyping.
Prevalence and determinants of gestational weight gain among pregnant women in Niger
Authors: Ouédraogo Césaire T. , Wessells K. Ryan , Young Rebecca R. , Faye M. Thierno , Hess Sonja Y. 
Published: October 2019

AbstractLow gestational weight gain (GWG) and low mid‐upper arm circumference (MUAC) are associated with adverse pregnancy outcomes. We aimed to assess the prevalence and determinants of low GWG and low MUAC among pregnant women in rural Zinder, Niger. A community‐based survey was conducted among 1,384 pregnant women in the catchment areas of 18 integrated health centers in the region of Zinder, Niger. Weight and MUAC were measured during an in‐home visit and again 1 month later, when haemoglobin concentration and micronutrient status were also assessed. The prevalence of low GWG was defined based on the 2009 United States Institute of Medicine (U.S. IOM) guidelines (<0.35 kg/week) and less than the third centile of the International Fetal and Newborn Growth Consortium for the 21st Century (INTERGROWTH‐21st) standards. Factors associated with GWG and MUAC were identified using logistic regression models adjusting for season, village, and gestational age. The median (interquartile range) age was 25.0 (20.7, 30.0) years, and 16.4% were ≤19 years. The prevalence of low GWG were 62.9% and 27.5% according to 2009 IOM and less than the third INTERGROWTH‐21st centile, respectively; 24.9% had low MUAC. Higher α‐1‐acid glycoprotein (OR = 1.7, 95% CI [1.1, 2.8]) and C‐reactive protein (OR = 1.2, 95% CI [1.02, 1.50]) increased the odds of low GWG. Adolescents (OR = 2.7, 95% CI [1.8, 4.0]), housewives (OR = 1.97, 95% CI [1.36, 2.86]), and those who reported recent food assistance (OR = 1.80, 95% CI [1.04, 3.11]) had higher odds of low MUAC. Prevalence of low GWG and low MUAC was high among pregnant women. Determinants of GWG and MUAC included socio‐economic, demographic, and biological factors, although only markers of inflammation were consistent predictors across different definitions of low GWG.
Demographic and environmental drivers of metagenomic viral diversity in vampire bats
Authors: Bergner Laura M. , Orton Richard J. , Benavides Julio A. , Becker Daniel J. , Tello Carlos , Biek Roman , Streicker Daniel G. 
Published: September 2019

AbstractViruses infect all forms of life and play critical roles as agents of disease, drivers of biochemical cycles and sources of genetic diversity for their hosts. Our understanding of viral diversity derives primarily from comparisons among host species, precluding insight into how intraspecific variation in host ecology affects viral communities or how predictable viral communities are across populations. Here we test spatial, demographic and environmental hypotheses explaining viral richness and community composition across populations of common vampire bats, which occur in diverse habitats of North, Central and South America. We demonstrate marked variation in viral communities that was not consistently predicted by a null model of declining community similarity with increasing spatial or genetic distances separating populations. We also find no evidence that larger bat colonies host greater viral diversity. Instead, viral diversity follows an elevational gradient, is enriched by juvenile‐biased age structure, and declines with local anthropogenic food resources as measured by livestock density. Our results establish the value of linking the modern influx of metagenomic sequence data with comparative ecology, reveal that snapshot views of viral diversity are unlikely to be representative at the species level, and affirm existing ecological theories that link host ecology not only to single pathogen dynamics but also to viral communities.
Optic disk protrusion on transocular ultrasonography is a new finding seen in patients with intracranial hypertension
Authors: Terasawa Yuka , Arai Ayumi , Sakai Kenichiro , Mitsumura Hidetaka , Iguchi Yasuyuki 
Published: September 2019

AbstractWe report a case with intracranial hypertension having new transocular ultrasonography finding. A forty‐five years old female with a long‐lasting headache and visual field abnormality was admitted to our hospital. She had bilateral papilloedema. After several examinations, she was diagnosed as idiopathic intracranial hypertension. She was examined with transocular ultrasonography to measure optic nerve sheaths diameter (ONSD). ONSD was 6.0mm on right side and 7.5mm on left side, and a new finding “optic disk protrusion” (ODP) was discovered. Three months after starting medication, her symptoms got better and her optic edema disappeared. She was re‐examined with transocular ultrasonography found the “ODP” had also disappeared. ODP would be simple finding to detect intracranial hypertension.
N‐acety‐b‐D‐glucosaminidase: A potential biomarker for early detection of acute kidney injury in acute chest pain
Authors: Kaufmann Manuel , Schlossbauer Michael , Hubauer Ute , Stadler Stefan , Fischer Marcus , Wallner Stefan , Hupf Julian , Zimmermann Markus , Orso Evelyn , Zeman Florian , Luchner Andreas , Maier Lars , Jungbauer Carsten 
Published: October 2019

AbstractAimAcute kidney injury (AKI) is often underdiagnosed due to several limitations of the renal marker creatinine. Tubular urinary biomarkers may substantially contribute to diagnose AKI early. For early detection of AKI, we evaluated for the first time N‐acetyl‐β‐d‐glucosaminidase (NAG), Kidney‐injury‐molecule‐1 (KIM‐1) and neutrophil gelatinase‐associated lipocalin (NGAL) in acute chest pain.MethodsWe included 402 chest pain patients aged 18 to 95 years seen in the emergency department. From 311 subjects, blood and urine samples were collected.ResultsThirty‐three patients developed an AKI and showed a significant increase in all three tubular markers compared to patients without AKI (eachP< .001). According to receiver operating characteristic (ROC) analysis, combining NAG and creatinine showed a significantly increased area under the curve (AUC) compared to creatinine alone (AUC: 0.75 vs 0.87;P< .001). KIM‐1, NGAL and cystatin C showed no significant differences in AUC compared to creatinine. In 120 individuals with blood and urine sampling before contrast media exposure, ROC analysis showed a significantly improved diagnostic performance for the combination of both (AUC: 0.83 vs creatinine AUC: 0.66;P= .004). AKI occurrence showed no dependency from CM volume. NAG presented as an independent AKI predictor beside creatinine, age, the diagnosis of myocardial infarction and mean arterial pressure. Regarding the prognostic value for renal replacement therapy, the combination of NAG and creatinine showed a significantly lager AUC than creatinine (AUC: 0.95 vs AUC: 0.85;P< .001).ConclusionNAG presented as a promising marker of impending AKI and the necessity of renal replacement therapy.
Hip Fractures: Therapy, Timing, and Complication Spectrum
Authors: Saul Dominik , Riekenberg Juliane , Ammon Jan C , Hoffmann Daniel B , Sehmisch Stephan 
Published: October 2019

ObjectiveInvestigation of the treatment of femur fractures and the type of femur fracture‐associated complications regarding timing of surgery and length of hospital stay.MethodsIn this retrospective cohort study, a total of 358 hip fractures were evaluated retrospectively from 1 January 2008 until 31 December 2010 at a level I trauma center in Germany. Inclusion criteria was age >18 years and a proximal femur fracture. Both sexes were evaluated. Mean age was 75.5 years, most patients were female (63.7%). Intervention was the operative treatment of proximal femur fracture. Outcome parameters were time until surgery, complications, reoperations, mortality, and length of hospital stay.ResultsAmong the proximal femur fractures (n = 358), 46.6% were pertrochanteric, 11.2% subtrochanteric, and 42.2% femoral neck fractures. Operation upon hip fractures was managed regularly within 24 hours of injury (73%; mean for femoral neck: 28.3 hrs.; mean for pertrochanteric fractures: 21.4 hrs.; mean for subtrochanteric fractures: 19.5 hrs.). Delayed treatment, as well as implantation of hip total endoprosthesis (TEP), increased the overall length of hospital stay (15.4 vs 17.6 days; 18.1 vs 15.8 days). Accordingly, surgical procedures performed within 24 hours of injury resulted in a shorter hospital residence. Longest delay of operation was measured for hip fractures (28.3 hrs.).In 351 patients, secondary injuries were detected in 94 individuals (26%), with fractures being the most common secondary injury (n = 40). We recorded postoperative complications of nonsurgical and surgical origin, and 33.6% of our patient cohort displayed complications. Complications were distributed among 118 patients. There was no significant difference in complications regarding the time of operation, with most nonsurgical and surgical complications appearing within 24 hours after operation (n = 110 vs n = 31). Nonsurgical complications, such as anemia (n = 49) and electrolyte imbalances (n = 30), were observed more frequently than surgical complications (n = 107 vs n = 34); however, these complications were reduced by delay in surgery (82.0% in 6–24 hrs. vs 74.2% in ≥24 hrs.). Anticoagulant therapy and age did not affect postoperative complications. The hospital mortality of patients was 6.2%. Follow‐up was restrained to ambulatory visits in the clinic.ConclusionsSurgical management of hip fractures performed within 24 hours of injury minimizes hospital stay. We did not detect significant differences in the spectrum or number of complications regarding delay of surgery. Surgical complications mainly occur with rapid primary care, and medical complications can be reduced by more intensive preparation of patient and operation procedures.