bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating the upper thermal limits of glochidia for selected freshwater mussel species (Bivalvia: Unionidae) in central and east Texas, and the implications for their conservation
Authors: Khan Jennifer M. , Hart Michael , Dudding Jack , Robertson Clinton R. , Lopez Roel , Randklev Charles R. 
Published: July 2019

Abstract

Understanding the temperature tolerances of organisms is critical because the thermal regimes of freshwater ecosystems are changing globally. Native freshwater mussels are sensitive to increasing water temperatures because of their physiology and unique life history. Detailed knowledge on lethal temperatures for mussels has been limited to less than 5% of the species known to occur in North America, and little is known about the thermal tolerances of mussel species from rivers within the south‐western USA.
To determine the effects of elevated water temperature on mussels, the upper thermal tolerances of larvae (glochidia) for the following species across four basins in Texas (Neches, Guadalupe, San Antonio, and Colorado) were tested: Amblema plicata, Cyclonaias necki, Fusconaia mitchelli, Lampsilis bracteata, Lampsilis hydiana, Lampsilis satura, Lampsilis teres, and Obovaria arkansasensis.
Glochidia were acclimated to 27°C across a range of experimental temperatures (30–39°C) in 24‐h standard acute laboratory tests. The median lethal temperature (LT50) among glochidia averaged 32.4°C and ranged from 26.9 to 36.4°C.
Thermal tolerances differed significantly among and within species, and by season. Comparing these results with current water temperatures in central and east Texas indicated that populations of the focal species studied are at risk from rising environmental temperatures and, as a consequence, their long‐term viability will be challenging in future years.
Using mixture‐amount modeling to optimize the advertising media mix and quantify cross‐media synergy for specific target groups
Authors: Goos Peter , Dens Nathalie , De Pelsmacker Patrick , Aleksandrovs Leonids 
Published: July 2019

AbstractOne of the critical decisions in media planning is how to allocate advertising efforts across different media. While studies indicate that marketers can create positive synergy effects by spreading their effort across several media, there is little understanding of how much should be invested in each specific medium to optimize advertising results. In this study, we apply a novel methodology, mixture‐amount modeling, which allows advertisers to determine the optimal allocation of advertising effort across media as a function of the total advertising effort. Moreover, we test how the optimal allocation and the resulting response change for consumers with distinctive media usage patterns and varying degrees of product category experience. Based on these results, we quantify the potential synergy between media and calculate the synergistic capacity for specific target groups. We apply the model to data from 52 beauty care advertising campaigns that ran on TV and in magazines in the Netherlands and Belgium. We determine the optimal allocation of advertising investments (measured through Gross Rating Points) to maximize campaign recognition. Our findings support the existence of positive synergistic effects between magazine and TV advertising and illustrate that these effects depend on consumers' media usage and product category experience.
The language network in autism: Atypical functional connectivity with default mode and visual regions
Authors: Gao Yangfeifei , Linke Annika , Jao Keehn Ruth Joanne , Punyamurthula Sanjana , Jahedi Afrooz , Gates Kathleen , Fishman Inna , Müller Ralph‐Axel 
Published: July 2019

Autism spectrum disorders (ASDs) are neurodevelopmental disorders associated with atypical brain connectivity. Although language abilities vary widely, they are impaired or atypical in most children with ASDs. Underlying brain mechanisms, however, are not fully understood. The present study examined intrinsic functional connectivity (iFC) of the extended language network in a cohort of 52 children and adolescents with ASDs (ages 8–18 years), using resting‐state functional magnetic resonance imaging. We found that, in comparison to typically developing peers (n = 50), children with ASDs showed increased connectivity between some language regions. In addition, seed‐to‐whole brain analyses revealed increased connectivity of language regions with posterior cingulate cortex (PCC) and visual regions in the ASD group. Post hoc effective connectivity analyses revealed a mediation effect of PCC on the iFC between bilateral inferior frontal and visual regions in an ASD subgroup. This finding qualifies and expands on previous reports of recruitment of visual areas in language processing in ASDs. In addition, increased iFC between PCC and visual regions was linked to lower language scores in this ASD subgroup, suggesting that increased connectivity with visual cortices, mediated by default mode regions, may be detrimental to language abilities. Autism Res 2019, 12: 1344–1355. © 2019 International Society for Autism Research, Wiley Periodicals, Inc.Lay SummaryWe examined the functional connectivity between regions of the language network in children with autism spectrum disorders (ASDs) compared to typically developing peers. We found connectivity to be intact between core language in the ASD group, but also showed abnormally increased connectivity between regions of an extended language network. Additionally, connectivity was increased with regions associated with brain networks responsible for self‐reflection and visual processing.
Synthesis of cinnabarinic acid by metabolically engineered Pseudomonas chlororaphis GP72
Authors: Yue Sheng‐Jie , Song Chen , Li Song , Huang Peng , Guo Shu‐Qi , Hu Hong‐Bo , Wang Wei , Zhang Xue‐Hong 
Published: July 2019

AbstractCinnabarinic acid is a valuable phenoxazinone that has broad applications in the pharmaceutical, chemical, and dyeing industries. However, few studies have investigated the production of cinnabarinic acid or its derivatives using genetically engineered microorganisms. Herein, an efficient synthetic pathway of cinnabarinic acid was designed and constructed in Pseudomonas chlororaphis GP72 for the first tim, which was more straightforward and robust than the known eukaryotic biosynthetic pathways. First, we screened and identified trans‐2,3‐dihydro‐3‐hydroxyanthranilic acid (DHHA) dehydrogenases from Escherichia coli MG1655 (encoded by entA), Streptomyces sp. NRRL12068 (encoded by bomO) and Streptomyces chartreusis NRRL3882 (encoded by calB3) based on the structural similarity of the substrate and product, and the DHHA dehydrogenase encoded by calB3 was selected for the synthesis of cinnabarinic acid due to its high DHHA conversion rate. Subsequently, cinnabarinic acid was synthesized by the expression of the DHHA dehydrogenase CalB3 and the phenoxazinone synthase CotA in the DHHA‐producing strain P. chlororaphis GP72, resulting in a cinnabarinic acid titer of 20.3 mg/L at 48 hr. Further fermentation optimization by the addition of Cu2+, H2O2, and with adding glycerol increased cinnabarinic acid titer to 136.2 mg/L in shake flasks. The results indicate that P. chlororaphis GP72 may be engineered as a microbial cell factory to produce cinnabarinic acid or its derivatives from renewable bioresources.
Hybrid physics‐based and data‐driven modeling for bioprocess online simulation and optimization
Authors: Zhang Dongda , Del Rio‐Chanona Ehecatl Antonio , Petsagkourakis Panagiotis , Wagner Jonathan 
Published: July 2019

AbstractModel‐based online optimization has not been widely applied to bioprocesses due to the challenges of modeling complex biological behaviors, low‐quality industrial measurements, and lack of visualization techniques for ongoing processes. This study proposes an innovative hybrid modeling framework which takes advantages of both physics‐based and data‐driven modeling for bioprocess online monitoring, prediction, and optimization. The framework initially generates high‐quality data by correcting raw process measurements via a physics‐based noise filter (a generally available simple kinetic model with high fitting but low predictive performance); then constructs a predictive data‐driven model to identify optimal control actions and predict discrete future bioprocess behaviors. Continuous future process trajectories are subsequently visualized by re‐fitting the simple kinetic model (soft sensor) using the data‐driven model predicted discrete future data points, enabling the accurate monitoring of ongoing processes at any operating time. This framework was tested to maximize fed‐batch microalgal lutein production by combining with different online optimization schemes and compared against the conventional open‐loop optimization technique. The optimal results using the proposed framework were found to be comparable to the theoretically best production, demonstrating its high predictive and flexible capabilities as well as its potential for industrial application.
Coexpression of CMP‐sialic acid transporter reduces N‐glycolylneuraminic acid levels of recombinant glycoproteins in Chinese hamster ovary cells
Authors: Kang Dong Gyun , Kim Chang Sup , Cha Hyung Joon 
Published: July 2019

AbstractRecombinant glycoproteins expressed in Chinese hamster ovary (CHO) cells contain two forms of sialic acids; N‐acetylneuraminic acid (Neu5Ac) as a major type and N‐glycolylneuraminic acid (Neu5Gc) as a minor type. The Neu5Gc glycan moieties in therapeutic glycoproteins can elicit immune responses because they do not exist in human. In the present work, to reduce Neu5Gc levels of recombinant glycoproteins from CHO cell cultures, we coexpressed cytidine‐5′‐monophosphate‐sialic acid transporter (CMP‐SAT) that is an antiporter and transports cytosolic CMP‐sialic acids (both forms) into Golgi lumen. When human erythropoietin was used as a target human glycoprotein, coexpression of CMP‐SAT resulted in a significant decrease of Neu5Gc level by 41.4% and a notable increase of Neu5Ac level by 21.2%. This result could be reasonably explained by our hypothesis that the turnover rate of Neu5Ac to Neu5Gc catalyzed by CMP‐Neu5Ac hydroxylase would be reduced through facilitated transportation of Neu5Ac into Golgi apparatus by coexpression of CMP‐SAT. We confirmed the effects of CMP‐SAT coexpression on the decrease of Neu5Gc level and the increase of Neu5Ac level using another glycoprotein human DNase I. Therefore, CMP‐SAT coexpression might be an effective strategy to reduce the levels of undesired Neu5Gc in recombinant therapeutic glycoproteins from CHO cell cultures.
Immuno-oncology for surgeons
Authors: Lee S L , Al-Shamkhani A , Mirnezami A 
Published: July 2019

Abstract
Cancer has traditionally been treated with surgery, cytotoxic chemotherapy and/or radiotherapy. The focus of treatment has been the mutated neoplastic cell. Critical advances in genomic and molecular techniques herald the potential for personalized treatments. Incremental breakthroughs in immunology have translated to a step-change in care by providing a mechanistic understanding of the immune system and how it may be mobilized to target cancer cells. As a result, clinical trials of immune-modifying agents have increased at an exponential rate and are revolutionizing cancer care. It is increasingly likely that the surgical oncologist will find themself caring for patients who have had immuno-oncology therapies as part of their neoadjuvant or adjuvant treatment. This review provides an update on immuno-oncology for the surgeon, covering the mechanisms of action of the agents in use. Emerging and surgically relevant toxicities are discussed, and available data on combining and sequencing cancer surgery with immuno-oncology treatments are summarized.
Revisiting the Language of Glycoscience: Readers, Writers and Erasers in Carbohydrate Biochemistry
Authors: Dedola Simone , Rugen Michael D. , Young Robert J. , Field Robert A. 
Published: July 2019

AbstractThe roles of carbohydrates in nature are many and varied. However, the lack of template encoding in glycoscience distances carbohydrate structure, and hence function, from gene sequence. This challenging situation is compounded by descriptors of carbohydrate structure and function that have tended to emphasise their complexity. Herein, we suggest that revising the language of glycoscience could make interdisciplinary discourse more accessible to all interested parties.
Asymmetric Synthesis of Adjacent Tri‐ and Tetrasubstituted Carbon Stereocenters: Organocatalytic Aldol Reaction of an Hydantoin Surrogate with Azaarene 2‐Carbaldehydes
Authors: Izquierdo June , Demurget Noémie , Landa Aitor , Brinck Tore , Mercero Jose M. , Dinér Peter , Oiarbide Mikel , Palomo Claudio 
Published: July 2019

AbstractA bifunctional amine/squaramide catalyst promoted direct aldol addition of an hydantoin surrogate to pyridine 2‐carbaldehyde N‐oxides to afford adducts bearing two vicinal tertiary/quaternary carbons in high diastereo‐ and enantioselectivity (d.r. up to >20:1; ee up to 98 %) is reported. Acid hydrolysis of adducts followed by reduction of the N‐oxide group yields enantiopure carbinol‐tethered quaternary hydantoin‐azaarene conjugates with densely functionalized skeletons. DFT studies of the potential energy surface (B3LYP/6–31+G(d)+CPCM (dichloromethane)) of the reaction correlate the activity of different catalysts and support an intramolecular hydrogen‐bond‐assisted activation of the squaramide moiety in the transition state of the catalytic reaction.
Contemporary benefit‐harm profile over two decades in primary prophylactic ICD‐therapy
Authors: Kleemann Thomas , Strauss Margit , Kouraki Kleopatra , Lampropoulou Eleni , Fendt Andràs , Werner Nicolas , Zahn Ralf 
Published: July 2019

AbstractBackgroundImplantable cardioverter defibrillator (ICD) was implemented into clinical routine more than 20 years ago. Since then, ICD therapy became standard therapy for primary and secondary prevention of sudden cardiac death in clinical practice.ObjectivesAim of the study was to evaluate the benefit‐harm profile of contemporary primary prophylactic ICD therapy.MethodsA total of 1222 consecutive patients of a prospective single‐center ICD‐registry were analyzed who underwent primary prophylactic ICD implantation between 2000 and 2017. Patients were divided into two groups according to the implantation year: 2010‐2017 (group 1, n = 579) and 2000‐2009 (group 2, n = 643).ResultsThe rate of estimated appropriate ICD therapy after 8 years was 51% in the 2000s and 42% in the 2010s (P < .001). The complication rate changed slightly from 53% to 47% (P = .005). This decline was mainly driven by the reduction of inappropriate ICD shocks (30% vs 14%, P < .001) whereas the rate of ICD shock lead malfunction and device/ lead infection remained unchanged over time. Nonischemic cardiomyopathy was an independent predictor for ICD complications without benefit of ICD therapy (HR 1.37, 95% CI 1.07‐1.77).ConclusionThe ICD therapy rate for ventricular arrhythmias in patients with primary prophylactic ICD implantation is decreasing over the last two decades. Complication rate remains high due to an unchanged rate of ICD shock malfunctions and device infections. Nonischemic cardiomyopathy is an independent predictor for ICD complications without benefit of ICD therapy in primary prophylactic ICD‐therapy.
The Pharmacokinetics and Relative Bioavailability of Mepolizumab 100 mg Liquid Formulation Administered Subcutaneously to Healthy Participants: A Randomized Trial
Authors: Shabbir Shaila , Pouliquen Isabelle J. , Bentley Jane H. , Bradford Eric S. , C. Kaisermann Morrys , Albayaty Muna 
Published: July 2019

AbstractThis study compared the pharmacokinetic (PK) profile of a new liquid formulation of mepolizumab with the established lyophilized formulation. In this open‐label, parallel‐group, single‐dose study (NCT03014674; GSK ID: 204958), healthy participants were randomized (1:1:1) to receive a single mepolizumab dose (100 mg) administered subcutaneously as liquid in a single‐use prefilled syringe or single‐use prefilled autoinjector, or as a lyophilized formulation. Maximum plasma concentration, area under the plasma concentration–time curve from time zero (predose) to time of last quantifiable concentration (AUC0–t), and AUC from time zero to infinity (AUC0–∞) as well as additional PK parameters, safety assessments, and blood eosinophil count were evaluated. In total, 244 participants received study drug. All PK parameters were similar across the 3 groups; 90% confidence intervals for maximum plasma concentration, AUC0–t, and AUC0–∞ treatment ratios (liquid prefilled syringe or autoinjector vs lyophilized formulation) were within conventional bioequivalence bounds (0.80‐1.25), demonstrating statistical PK comparability. On‐treatment adverse event incidence was 29% to 38%. Mepolizumab liquid formulation administered via prefilled syringe or autoinjector had similar PK properties to the lyophilized formulation, with no safety concerns identified.
Practical Considerations for Continuum Models Applied to Surface Electrochemistry
Authors: Gauthier Joseph A. , Dickens Colin F. , Ringe Stefan , Chan Karen 
Published: July 2019

AbstractModelling the electrolyte at the electrochemical interface remains a major challenge in ab initio simulations of charge transfer processes at surfaces. Recently, the development of hybrid polarizable continuum models/ab initio models have allowed for the treatment of solvation and electrolyte charge in a computationally efficient way. However, challenges remain in its application. Recent literature has reported that large cell heights are required to reach convergence, which presents a serious computational cost. Furthermore, calculations of reaction energetics require costly iterations to tune the surface charge to the desired potential. In this work, we present a simple capacitor model of the interface that illuminates how to circumvent both of these challenges. We derive a correction to the energy for finite cell heights to obtain the large cell energies at no additional computational expense. We furthermore demonstrate that the reaction energetics determined at constant charge are easily mapped to those at constant potential, which eliminates the need to apply iterative schemes to tune the system to a constant potential. These developments together represent more than an order of magnitude reduction of the computational overhead required for the application of polarizable continuum models to surface electrochemistry.
Privacy‐preserving authentication scheme for on‐road on‐demand refilling of pseudonym in VANET
Authors: Benarous Leila , Kadri Benamar , Bitam Salim , Mellouk Abdelhamid 
Published: July 2019

SummaryPrivacy in Vehicular Ad Hoc Networks (VANET) is fundamental because the user's safety may be threatened by the identity and the real‐time spatiotemporal data exchanged on the network. This issue is commonly addressed by the use of certified temporal pseudonyms and their updating strategies to ensure the user's unlinkability and anonymity. IEEE 1609.2 Standard specified the process of certifying pseudonym along with certificates structure. However, the communication procedure between the certifying authority and the requesting vehicle was not defined. In this paper, a new privacy‐preserving solution for pseudonym on‐road on‐demand refilling is proposed where the vehicle anonymously authenticates itself to the regional authority subsidiary of the central trusted authority to request a new pseudonyms pool. The authentication method has two phases, the first one uses anonymous tickets, and the second one is a challenge‐based authentication. The anonymous tickets are certificates that do not include the identity of the user. Instead, it contains a reference number and the certifying authority signature. The challenge authentication is identity‐less to preserve the privacy, yet it is used to prevent the misuse of tickets and the impersonation of its owner. Our proposed scheme is analyzed by the use of Burrows, Abadi and Needham (BAN) logic to demonstrate its correctness. It is also specified and checked by using the Security Protocol ANimator (SPAN) and the Automated Validation of Internet Security Protocols and Applications (AVISPA) tools. The logical demonstration proved that this privacy‐preserving authentication is assured. The SPAN and AVISPA tools illustrated that it is resilient to security attacks.
Variability in remission in family therapy for anorexia nervosa
Authors: Le Grange Daniel , Huryk Kathryn M. , Murray Stuart B. , Hughes Elizabeth K. , Sawyer Susan M. , Loeb Katharine L. 
Published: July 2019

AbstractObjectiveThe evolution toward more stringent conceptualizations of remission in family therapy for adolescent anorexia nervosa (AN) has, with time, introduced variability in outcomes across randomized controlled trials (RCTs). An examination of remission across the history of research on family therapy for AN shows that earlier studies adopted lenient definitions and generally yielded higher rates of remission than studies of the past decade that have used stricter definitions of remission. In this study, we investigate the reactivity of remission rates to the application of different definitions of remission used within the family therapy for AN literature, within a single RCT data set.MethodWe conducted a secondary analysis of data from a single‐site RCT which compared the relative efficacy of two formats of family therapy in a sample of 106 Australian adolescents with AN. Using end‐of‐treatment data, we compared remission rates using 11 definitions of remission that have been used in studies of family therapy for AN spanning more than three decades.ResultsWe found wide variability in remission rates (21.7–87.7%; Cochran's Q χ2(10, N = 106) = 303.55, p = .000], depending on which definition of remission was applied. As expected, more lenient criteria produced higher remission rates than more stringent definitions.DiscussionApplying different criteria of remission to a single data set illustrates the impact of changing how remission is defined. Failure to consider the greater stringency of remission criteria in recent studies could result in false inferences concerning the efficacy of family therapy for AN over time.
Convergence in maternal and child reports of impulsivity, depressive symptoms, and trait anxiety, and their predictive utility for binge‐eating behaviors
Authors: Vo Phuong T. , Racine Sarah E. , Burt Sybil Alexandra , Klump Kelly L. 
Published: July 2019

AbstractObjectiveEarly detection of binge‐eating (BE) behaviors and their risk factors is associated with better outcomes. A multi‐informant approach for assessing BE psychopathology and risk factors has been emphasized to increase the probability and accuracy of early detection. Impulsivity (particularly negative and positive urgency), trait anxiety, and depressive symptoms are associated with BE behaviors. The present study examined maternal‐child convergence of reports of child BE, impulsivity, trait anxiety, and depressive symptoms and examined the predictive power of maternal reports for child‐reported BE behaviors.MethodParticipants included 927 female twins (aged 8–16 years) and 468 mothers from the Michigan State University Twin Registry. Risk factors and BE were assessed with self‐report questionnaires.ResultsIntraclass correlation coefficients showed fair‐to‐moderate inter‐rater agreement (ICCs = .31–.41) between maternal and child reports of risk factors and low‐to‐fair agreement for BE (ICCs = .05–.29). Controlling for the effects of age, pubertal status, body mass index, and family relatedness, multilevel models showed that maternal reports of child impulsivity, anxiety, and depressive symptoms did not add predictive power above and beyond child reports.DiscussionResults call into question the utility and practical implications of using maternal reports to supplement child reports for BE and its risk factors.