bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retracted: Effect of microRNA‐186 on oxidative stress injury of neuron by targeting interleukin 2 through the janus kinase‐signal transducer and activator of transcription pathway in a rat model of Alzheimer’s disease
Authors: Wu Dong‐Mei , Wen Xin , Wang Yong‐Jian , Han Xin‐Rui , Wang Shan , Shen Min , Fan Shao‐Hua , Zhuang Juan , Zhang Zi‐Feng , Shan Qun , Li Meng‐Qiu , Hu Bin , Sun Chun‐Hui , Lu Jun , Chen Gui‐Quan , Zheng Yuan‐Lin 
Published: July 2018

Recent studies have proposed that microRNAs (miR) function as novel diagnostic and prognostic biomarkers and therapeutic targets in Alzheimer’s disease (AD), a common disease among the elderly. In the current study, we aim to explore the effect of miR‐186 on oxidative stress injury of neuron in rat models of AD with the involvement of the interleukin‐2 (IL2) and the Janus kinase/signal transducers and activators of transcription (JAK–STAT) pathways. AD rat models were established, and dual‐luciferase reporter assay and online software were used to confirm the targeting relationship between miR‐186 and IL2. Immunohistochemistry was used evaluating the positive rate of IL2. Afterward, to define the role of miR‐186 in AD, miR‐186, IL2, and JAK–STAT related protein (JAK2, STAT3) expressions were quantified. Cell proliferation was measured by 3‐(4,5‐dimethylthiazol‐2‐yl)2,5‐diphenyl tetrazolium bromide, and cell apoptosis was detected by flow cytometry. We observed downregulated miR‐186 and IL2 and upregulated JAK–STAT signaling pathway related genes in AD. The overexpression of miR‐186 was shown to significantly promote cell proliferation while suppressing cell apoptosis along with the expression of the IL2 and JAK–STAT signaling pathway related protein. Collectively, the key findings obtained from the current study define the potential role of miR‐186 as an inhibitor of AD development by downregulation of IL2 through suppression of the JAK–STAT signaling pathway.
Participatory research—A modernizing science for primary health care
Authors: Andersson Neil 
Published: July 2018

AbstractParticipatory research is the science of partnerships underlying research, concerned with research governance, ownership of research products, and relationships behind research objectives and methods. The common strand behind the quite different schools of participatory research is that research should be in respectful partnership with people; it is not about researchers working on, for, or about people. Modern participatory research embraces different philosophies through several applications. The first application addresses research objectives, with participation at different points in the research cycle. Second, modern participatory research is relevant in adaptive management, including management of primary health care. Third, participatory research is a tool for patient engagement and patient‐centered outcomes in the clinical context. A fourth application is participatory research as an intervention: Participatory research moves people, and it mobilizes resources and can thus be pivotal to sustainability and for health‐promoting intersectoral linkages. As primary health care is a family medicine responsibility, participatory research offers family medicine a valuable toolbox complementing the accepted clinical toolboxes. Through shared identification of problems and decisions about solutions, participatory research increases participant capacity to identify and address their own issues. Among clinicians, it enhances professional practices. In the bigger social picture, all this promotes social justice, self‐determination, and knowledge utilization.
Hematopoietic cell-derived RELMα regulates hookworm immunity through effects on macrophages
Authors: Batugedara Hashini M , Li Jiang , Chen Gang , Lu Dihong , Patel Jay J , Jang Jessica C , Radecki Kelly C , Burr Abigail C , Lo David D , Dillman Adler R , Nair Meera G 
Published: July 2018

Abstract
Resistin-like molecule α (RELMα) is a highly secreted protein in type 2 (Th2) cytokine-induced inflammation including helminth infection and allergy. In infection with Nippostrongylus brasiliensis (Nb), RELMα dampens Th2 inflammatory responses. RELMα is expressed by immune cells, and by epithelial cells (EC); however, the functional impact of immune versus EC-derived RELMα is unknown. We generated bone marrow (BM) chimeras that were RELMα deficient (RELMα−/−) in BM or non BM cells and infected them with Nb. Non BM RELMα−/− chimeras had comparable inflammatory responses and parasite burdens to RELMα+/+ mice. In contrast, both RELMα−/− and BM RELMα−/− mice exhibited increased Nb-induced lung and intestinal inflammation, correlated with elevated Th2 cytokines and Nb killing. CD11c+ lung macrophages were the dominant BM-derived source of RELMα and can mediate Nb killing. Therefore, we employed a macrophage-worm co-culture system to investigate whether RELMα regulates macrophage-mediated Nb killing. Compared to RELMα+/+ macrophages, RELMα−/− macrophages exhibited increased binding to Nb and functionally impaired Nb development. Supplementation with recombinant RELMα partially reversed this phenotype. Gene expression analysis revealed that RELMα decreased cell adhesion and Fc receptor signaling pathways, which are associated with macrophage-mediated helminth killing. Collectively, these studies demonstrate that BM-derived RELMα is necessary and sufficient to dampen Nb immune responses, and identify that one mechanism of action of RELMα is through inhibiting macrophage recruitment and interaction with Nb. Our findings suggest that RELMα acts as an immune brake that provides mutually beneficial effects for the host and parasite by limiting tissue damage and delaying parasite expulsion.
Employing hookworm infection of RELMα−/− bone marrow chimeras, co-culture assays, and gene expression analysis, we show that lung macrophage-derived RELMα downregulates inflammation and parasite killing.
Development of an ASE‐GC‐MS/MS method for detecting dinitolmide and its metabolite 3‐ANOT in eggs
Authors: Zhao Xia , Wang Bo , Xie Kaizhou , Liu Yanan , Zhang Yangyang , Wang Yajuan , Liu Chujun , Guo Yawen , Bu Xiaona , Zhang Genxi , Zhang Tao , Dai Guojun 
Published: July 2018

AbstractAn accelerated solvent extraction coupled with gas chromatography‐tandem mass spectrometry (ASE‐GC‐MS/MS) method for detecting dinitolmide residue and its metabolite (3‐amino‐2‐methyl‐5‐nitrobenzamide, 3‐ANOT) in eggs was developed and optimized. The samples were extracted using ASE with acetonitrile as the extractant and were purified by passage through a neutral alumina solid‐phase extraction column. Then, the samples were analyzed using the GC‐MS/MS method. The optimized method parameters were validated according to the requirements set forth by the European Union and the Food and Drug Administration. The average recoveries of dinitolmide and 3‐ANOT from eggs (egg white, egg yolk, and whole egg) at the limit of quantification (LOQ), 0.5 maximum residue limit (MRL), 1 MRL, and 2 MRL were 82.74% to 87.49%, the relative standard deviations (RSDs) were less than 4.63%, and the intra‐day RSDs and the inter‐day RSDs were 2.96% to 5.21% and 3.94% to 6.34%, respectively. The limits of detection and the LOQ were 0.8 to 2.8 μg/kg and 3.0 to 10.0 μg/kg, respectively. The decision limits (CCα) were 3001.69 to 3006.48 μg/kg, and the detection capabilities (CCβ) were 3001.74 to 3005.22 μg/kg. Finally, the new method was successfully applied to the quantitative determination of dinitolmide and 3‐ANOT in 50 commercial eggs from local supermarkets.
Natural course of persistent hepatitis B virus infection in hepatitis B e antigen‐positive and hepatitis B e antigen‐negative cohorts in Japan based on the Markov model
Authors: Yamasaki Kazumi , Tanaka Junko , Kurisu Akemi , Akita Tomoyuki , Ohisa Masayuki , Sakamune Kazuaki , Ko Ko , Sugiyama Aya , Yasaka Takahiro , Shirahama Satoshi 
Published: July 2018

AbstractThis population‐based study examined the natural course of hepatitis B e antigen (HBeAg)‐positive or HBeAg‐negative persistent hepatitis B virus (HBV) infection, adjusted by age and liver disease states using a Markov model. Using 12 417 person‐years data (n = 862), annual transition probabilities were estimated, and age‐adjusted cumulative incidence and natural history of persistent HBV infection were simulated in both sexes of groups 1 (HBeAg‐negative status with HBV DNA level <4.0 log IU/mL at entry) and 2 (persistent HBeAg‐positive status throughout the study). In group 1, 15.26% of 30‐years old men with chronic hepatitis (CH) were expected to remain in the same state at age 65 years, 28.32% subsided into an hepatitis B surface antigen (HBsAg)‐negative state, and 13.20% developed hepatocellular carcinoma (HCC). The expectations for 40‐years old men in group 1 were 21.43%, 19.86%, and 15.04%, respectively. The expectations for 30 years women in group 1 were 30.57%, 21.15%, and 4.08%, respectively. These results suggest that HBeAg positivity caused a higher risk of HCC onset in persistent HBV infection after adjustments for age, sex, and liver disease state. HCC was likely to develop, but unlikely to subside into HBsAg clearance, remaining in a CH state with aging, regardless of HBeAg state. Furthermore, both HCC development and HBsAg clearance occurred more frequently in men than in women, irrespective of HBeAg status.
A multi‐state, time‐removal model for population dynamics of cliff‐nesting raptors
Authors: Mizel Jeremy D. , Mcintyre Carol L. , Lewis Stephen B. , Lindberg Mark S. , Schmidt Joshua H. 
Published: July 2018

ABSTRACTCliff‐nesting raptors present considerable challenges for population estimation due to their sparse distribution across remote landscapes and the multiple occupancy states (e.g., unoccupied, occupancy without breeding, breeding occupancy) through which we observe their nesting territory dynamics. To increase the efficiency and spatial inference of surveys, we developed 2 versions of a multi‐state, time‐removal model: 1 for long‐term monitoring studies and the other for population inventories or single‐season surveys in which there is no prior knowledge of nest locations. We focused our development of these methods in the context of a combined aerial and ground‐based survey approach, which permits efficient surveying at landscape scales. The approach, however, is also applicable to designs restricted to ground surveys. For long‐term monitoring of species with alternative nests, we formulated a version of the model that accounts for state uncertainty at the territory level caused by a failure to observe all nests within a territory. Simulations based on the long‐term monitoring model indicated adequate (near nominal) coverage and low relative bias (<0.05) for nearly all parameters. In the simulation study for the inventory model, population size estimates showed negligible bias for a survey duration of 90 minutes. We applied our approach to a long‐term study of golden eagles (Aquila chrysaetos) in Alaska and demonstrated that the maximum effort spent on any nesting territory could be reduced by up to almost 90% of that recommended by standard protocols. Published 2018. This article is a U.S. Government work and is in the public domain in the USA.
Quantifying the effects of human activities and climate variability on vegetation cover change in a hyper‐arid endorheic basin
Authors: Shen Qin , Gao Guangyao , Han Fei , Xiao Feiyan , Ma Ying , Wang Shuai , Fu Bojie 
Published: July 2018

AbstractQuantifying the contributions of human activities and climatic factors to vegetation cover change is critical for sustainable ecosystem and water resources management in arid and semiarid regions. In this study, the temporal–spatial variations of normalized difference vegetation index (NDVI) in the middle reaches of the Heihe River Basin (a typical arid endorheic basin in China) from 2000 to 2015 were detected, and a formal analytic framework was used to quantify the roles of climatic factors and human activities in driving vegetation cover change. The results showed that the study area has undergone evident overall greening with significant increases in NDVI in both oasis and desert areas. The fractional oasis area increased significantly from 22.93% to 27.69%, and the desert area decreased. The overall greening was mainly due to increases of fractional oasis area and NDVI in desert area with contributions of 50.50% and 42.08%, respectively. The increase in regional NDVI was mainly driven by gross domestic product (GDP) and precipitation with contributions of 40.2% and 40.3%, respectively, and the watertable played a secondary role in the NDVI increase with a contribution of less than 20%. This study indicated that human activities (i.e., oasis expansion) in the oasis areas and precipitation in the desert areas played decisive roles in regional NDVI changes. Coordination between expanding oases and maintaining the stability of oasis ecosystems should be reached. This study provides important references that policy makers and stakeholders can use to rationally manage ecosystem restoration and socioeconomic development and prevent land degradation in endorheic basins.
Catch Trend and Stock Assessment of Hilsa Tenualosa ilisha Using Digital Image Measured Length-Frequency Data
Authors: Rahman Mohammad Jalilur , Wahab Md. Abdul , Amin S. M. Nurul , Nahiduzzaman Md. , Romano Nicholas 
Published: July 2018

Abstract
The annual global average catch shares of the anadromous Hilsa Tenualosa ilisha of Bangladesh increased rapidly from 74.5% all through 1984–2013 to 86.7% during the 2010–2015 periods. With a few exceptions, an increasing trend of Hilsa production over the last three decades was found in Bangladesh. Initially three options incorporating digital image measurements were compared to determine the best method for obtaining accurate length data. The length-frequency data measured from digital images showed that Hilsa have a moderate growth rate (K =0.90 year−1) of up to 58.70 cm (L) TL. High fishing mortality (F =2.83 year−1) and exploitation level (E =0.67) suggest a slight overexploitation of the Hilsa fishery; the maximum sustainable yield (MSY) was estimated at 526,000 metric tons/year if the recommended TL at first capture (Lc) of 27 cm is adhered to. The present annual Hilsa catch is about 496,417 metric tons, which indicates the potential benefit of achieving MSY through sustainable fisheries management by regulating mesh size of nets and protecting brood fish. Size distribution of Hilsa within sanctuaries revealed a remarkable presence of juvenile fish during February–March in some areas, which suggests a need to readjust the fishing ban period from March–April to February–March. More fisheries management is necessary to reduce the exploitation level of Hilsa by 17% and at the same time increase the allowable Lc to potentially increase the MSY and CPUE.
Applying Bayesian approach to predict deformations during tunnel construction
Authors: Janda Tomáš , Šejnoha Michal , Šejnoha Jiří 
Published: July 2018

SummaryIn this paper a fully probabilistic approach based on the Bayesian statistical method is presented to predict ground settlements in both transverse and longitudinal directions during gradual excavation of a tunnel. To that end, the convergence confinement method is adopted to give estimates of ground deformation numerically. Together with in situ measurements of the evolution of vertical deflections at selected points along the tunnel line, it allows for the construction of a likelihood function and consequently in the framework of Bayesian inference to provide posterior improved knowledge of model parameters entering the numerical analysis. In this regard, the Bayesian updating is first exploited in the material identification step and next used to yield predictions of ground settlement in sections along the tunnel line ahead of the tunnel face. This methodology thus makes it possible to improve original designs by utilizing an increasing number of data (measurements) collected in the course of tunnel construction.
Genipin crosslinked microspheres as an effective hemostatic agent
Authors: Liu Wen , Yang Xiao , Li Na , Xi Guanghui , Wang Mingshan , Liang Bin , Feng Yakai , Chen Hao , Shi Changcan , Li Wenzhong 
Published: July 2018

Efficient hemorrhage control is of extreme importance to decrease mortality rates arising from traumatic bleeding. However, traditional hemostatic agents are limited to unsatisfied biocompatibility, inconvenient usage, and high cost. To this end, we have devised a novel hemostatic microsphere from chitosan and gelatin by using appropriate amount of genipin as a crosslinker. The performance of these Cs/Gel MPs was evaluated with experiments in vitro and in vivo. Results in vitro showed that the best hemostatic efficacy was achieved by Cs/Gel‐0.75 MPs (the mass fraction of genipin in Cs/Gel mixture is 8.6 × 10−6). Skin laceration model indicated that the hemostatic time of Cs/Gel‐0.75 MPs was within 12 seconds, which has been significantly shortened in comparison with commercial available products. Moreover, cytocompatibility results displayed that Cs/Gel‐0.75 MPs caused no obvious cytotoxicity even at the high concentration of 40 μg/ml. Cs/Gel‐0.75 MPs also possess satisfactory fluid absorption ratio and low hemolysis. Therefore, these Cs/Gel‐0.75 MPs are promising candidates for further surgical wound hemostatic agent.
Plasma Proteome Profiles of Stable CAD Patients Stratified According to Total Apo C‐III Levels
Authors: Manfredi Marcello , Chiariello Carmela , Conte Eleonora , Castagna Annalisa , Robotti Elisa , Gosetti Fabio , Patrone Mauro , Martinelli Nicola , Bassi Antonella , Cecconi Daniela , Marengo Emilio , Olivieri Oliviero 
Published: July 2018

PurposeThe present research reports the study the of plasma proteome profile of stable coronary artery disease (CAD) patients characterized by different levels of total Apolipoprotein‐CIII (Apo C‐III), a prognostic marker for cardiovascular risk.Experimental designTwo subgroups of CAD patients (n = 52) with divergent concentrations of total circulating Apo C‐III (≤ and ≥10 mg dL−1) are examined using a shotgun proteomic approach. Validation experiments are also performed with immunochemistry methods including both the patients affected by CAD (n = 119) and the subjects without CAD (CAD‐free; n = 58). Results are analyzed by bioinformatics tools and multivariate statistics.ResultsA total of 188 proteins are quantified among the patients. The fold change analysis and the partial least square discriminant analysis show a clear separation of the two groups. Lipoproteins (Apo C‐II and Apo E), retinol‐binding protein 4, and vitronectin are upregulated in patients with high Apo C‐III, while alpha‐1 antitrypsin is downregulated.Conclusions and clinical relevanceIn this pilot study, the differential expression of plasma proteins related to different concentrations of Apo C‐III is defined, suggesting possible new players involved in the Apo C‐III‐associated process of arterial damage. Data are available via ProteomeXchange with identifier PXD005973.
Photothermal Control of Heat‐Shock Protein Expression at the Single Cell Level
Authors: Robert Hadrien M. L. , Savatier Julien , Vial Stéphanie , Verghese Jacob , Wattellier Benoit , Rigneault Hervé , Monneret Serge , Polleux Julien , Baffou Guillaume 
Published: July 2018

AbstractLaser heating of individual cells in culture recently led to seminal studies in cell poration, fusion, migration, or nanosurgery, although measuring the local temperature increase in such experiments remains a challenge. Here, the laser‐induced dynamical control of the heat‐shock response is demonstrated at the single cell level, enabled by the use of light‐absorbing gold nanoparticles as nanosources of heat and a temperature mapping technique based on quadriwave lateral shearing interferometry (QLSI) measurements. As it is label‐free, this approach does not suffer from artifacts inherent to previously reported fluorescence‐based temperature‐mapping techniques and enables the use of any standard fluorescent labels to monitor in parallel the cell's response.
Roux‐en‐Y gastric bypass surgery normalizes dopamine D1, D2, and DAT levels
Authors: Hamilton John , Swenson Sabrina , Hajnal Andras , Thanos Panayotis K. 
Published: July 2018

AbstractRoux‐en‐Y gastric bypass surgery (RYGB) is one of the most effective treatments for morbid obesity. However, increased substance abuse following RYGB has been observed clinically. This study examined the effects of RYGB on the dopamine system to elucidate these observed changes in reward‐related behavior. Rats were assigned to four groups: normal diet with sham surgery, ad libitum high fat (HF) diet with sham surgery, restricted HF diet with sham surgery, and HF diet with RYGB surgery. Following surgeries, rats were kept on their respective diets for 9 weeks before they were sacrificed. [3H]SCH 23390, [3H]Spiperone, and [3H]WIN35 428 autoradiography was performed to quantify the effects of diet and RYGB surgery on dopamine type 1‐like receptor (D1R)‐like, dopamine type 2‐like receptor (D2R)‐like, and dopamine transporter (DAT) binding. Rats on a chronic HF diet became obese with reduced D1R‐like binding within the ventrolateral striatum and the nucleus accumbens core, reduced D2R‐like binding in all areas of the striatum and nucleus accumbens core and shell, and reduced DAT binding in the dorsomedial striatum. Restricted HF diet rats showed similar reductions in D1R‐like and D2‐R‐like binding as the obese rats, and reduced DAT binding within all areas of the striatum. Both RYGB and restricted HF diet rats showed similar weight reductions, with RYGB rats showing no difference in binding compared to controls. The observed changes in binding between non‐treated obese rats and RYGB rats demonstrates that HF dietary effects on the dopamine system were reversed by RYGB.
Explicitly correlated local coupled‐cluster methods using pair natural orbitals
Authors: Ma Qianli , Werner Hans‐Joachim 
Published: July 2018

Recently developed explicitly correlated local coupled‐cluster methods [PNO‐LCCSD(T)‐F12] are reviewed. Extensive benchmarks for reaction energies and intermolecular interaction energies are presented, in which the convergence of the results with respect to all local approximations is studied. The explicit correlation treatment (F12) is shown to be essential to minimize basis set incompleteness errors, as well as errors caused by domain approximations. Generally, the errors of relative energies due to local approximations can be reduced to below 1 kcal/mol. The methods are well parallelized, and using small computer clusters with 100–200 computing cores, calculations for systems with 100–200 atoms using augmented triple‐ζ basis sets can be carried out within a few hours of elapsed time. Recommendations are made on how such calculations should be carried out, how the accuracy can be tested, and which computational resources are required.This article is categorized under:

Electronic Structure Theory> Ab Initio Electronic Structure Methods
Software> Quantum Chemistry