bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D Fluorescence Spectroscopy Combined With Chemometrics as a Tool for Control of Imprinted Protein Purification From Template Molecules
Authors: Burmistrova Natalia A. , Ilicheva Polina M. , Presnyakov Kirill Yu. , Pidenko Pavel S. , Rutledge Douglas N. 
Published: October 2024

ABSTRACTImprinted proteins (IPs) are promising alternatives to natural recognition systems, such as biological receptors or antibodies. One of the crucial stages during development of IPs is removal of the template molecules from its complex with the protein. In this study, bovine serum albumin was imprinted in the presence of 4‐hydroxycoumarin (4‐HC); purification of IPs were carried out by dialysis, and fluorescence 3D spectroscopy was used to monitor the IP purification process. Excitation–emission matrix (EEM) was further investigated via several chemometric algorithms (principal component analysis [PCA], parallel factor analysis [PARAFAC], and independent components analysis [ICA]). We found that the models using PARAFAC and ICA worked better than those of PCA. It was shown that PARAFAC and ICA analyses allow not only to recognize IP sample with signal close to nonimprinted protein, but also to provide recommendations on the optimal dialysis time.
Modeling an Industrial Hydrochlorination of Glycerol – A Systematic Study of Basic Reactor Types
Authors: Baum Philipp Adrian , Gerlach Martin , Hamel Christof 
Published: October 2024

AbstractModeling and optimization of real chemical reactors is challenging. A program to systematically compare the performance of an existing reactor with basic reactor models was built. The program was tested on the hydrochlorination of bio‐based glycerol in a bubble column. The model‐based approach provided insights into the reactor's concentration profiles, mass transfer, and mixing. In this case study, a plug‐flow tubular reactor (PFTR) is sufficient as a model for process intensification. Based on the results, a novel reactor design that significantly improves yields was proposed and simulated.
Understanding the Mineral Nutrient Value of Wheat Residue
Authors: Adams Curtis B. , Rogers Christopher W. , Marshall Juliet M. , Hatzenbuehler Patrick , Walsh Olga S. , Thurgood Garrett , Dari Biswanath , Loomis Grant , Tarkalson David 
Published: October 2024

AbstractThere is a substantial pool of mineral nutrients contained in wheat residue, concentrated in K, which has substantial economic value. Given this value, it is important for wheat producers to weigh the relative benefits of residue harvest, which gives immediate but marginal revenue gains, and residue retention, which has multifaceted benefits that include substantial savings on future nutrient costs. Persistent removal of nutrients from agronomic systems through residue harvest affects soil nutrient availability in the short‐ and long‐term, and the timing and magnitude of these changes will depend on the cropping system and soil. Earn 1 CEU in Nutrient Management by reading the article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.
Spatially resolved metabolomics: From metabolite mapping to function visualising
Authors: Min Xinyue , Zhao Yiran , Yu Meng , Zhang Wenchao , Jiang Xinyi , Guo Kaijing , Wang Xiangyi , Huang Jianpeng , Li Tong , Sun Lixin , He Jiuming 
Published: October 2024

AbstractMass spectrometry imaging (MSI)‐based spatially resolved metabolomics addresses the limitations inherent in traditional liquid chromatography‐tandem mass spectrometry (LC–MS)‐based metabolomics, particularly the loss of spatial context within heterogeneous tissues. MSI not only enhances our understanding of disease aetiology but also aids in the identification of biomarkers and the assessment of drug toxicity and therapeutic efficacy by converting invisible metabolites and biological networks into visually rendered image data. In this comprehensive review, we illuminate the key advancements in MSI‐driven spatially resolved metabolomics over the past few years. We first outline recent innovations in preprocessing methodologies and MSI instrumentation that improve the sensitivity and comprehensiveness of metabolite detection. We then delve into the progress made in functional visualization techniques, which enhance the precision of metabolite identification and annotation. Ultimately, we discuss the significant potential applications of spatially resolved metabolomics technology in translational medicine and drug development, offering new perspectives for future research and clinical translation.Highlights
MSI‐driven spatial metabolomics preserves metabolite spatial information, enhancing disease analysis and biomarker discovery.
Advances in MSI technology improve detection sensitivity and accuracy, expanding bioanalytical applications.
Enhanced visualization techniques refine metabolite identification and spatial distribution analysis.
Integration of MSI with AI promises to advance precision medicine and accelerate drug development.
Lactylation in cancer: Mechanisms in tumour biology and therapeutic potentials
Authors: He Yipeng , Song Tianbao , Ning Jinzhuo , Wang Zefeng , Yin Zhen , Jiang Pengcheng , Yuan Qin , Yu Weimin , Cheng Fan 
Published: October 2024

AbstractLactylation, a recently identified form of protein post‐translational modification (PTM), has emerged as a key player in cancer biology. The Warburg effect, a hallmark of tumour metabolism, underscores the significance of lactylation in cancer progression. By regulating gene transcription and protein function, lactylation facilitates metabolic reprogramming, enabling tumours to adapt to nutrient limitations and sustain rapid growth. Over the past decade, extensive research has revealed the intricate regulatory network underlying lactylation in tumours. Large‐scale sequencing and machine learning have confirmed the widespread occurrence of lactylation sites across the tumour proteome. Targeting lactylation enzymes or metabolic pathways has demonstrated promising anti‐tumour effects, highlighting the therapeutic potential of this modification. This review comprehensively explores the mechanisms of lactylation in cancer cells and the tumour microenvironment. We expound on the application of advanced omics technologies for target identification and data modelling within the lactylation field. Additionally, we summarise existing anti‐lactylation drugs and discuss their clinical implications. By providing a comprehensive overview of recent advancements, this review aims to stimulate innovative research and accelerate the translation of lactylation‐based therapies into clinical practice.Key points
Lactylation significantly influences tumour metabolism and gene regulation, contributing to cancer progression.
Advanced sequencing and machine learning reveal widespread lactylation sites in tumours.
Targeting lactylation enzymes shows promise in enhancing anti‐tumour drug efficacy and overcoming chemotherapy resistance.
This review outlines the clinical implications and future research directions of lactylation in oncology.
Considering Sufficient Weight Loss as a Prerequisite for Development of Anorexia Nervosa and Atypical Anorexia Nervosa
Authors: Hebebrand Johannes , Seitz Jochen , Matthews Abigail 
Published: October 2024

ABSTRACTBackgroundBecause premorbid BMI strongly predicts the amount of weight loss in anorexia nervosa (AN)/atypical AN, we hypothesize that weight loss triggers both disorders by inducing the endocrine adaptation to starvation.MethodWe propose research to capture the onset of AN/atypical AN following intentional, otherwise behaviorally motivated, or unintentional weight loss in relationship to premorbid weight.ResultsWe propose retrospective and prospective studies to examine the temporal development of symptoms in AN/atypical AN. Given a greater frequency of weight loss behaviors in individuals with high BMIs, patients with intentionally driven weight loss should demonstrate a higher mean weight loss and greater premorbid weight and shape concerns. Practice guidelines necessitate weight gain for AN recovery, yet how weight gain induces improvements is unspecified and warrants systematic elucidation. Finally, we highlight implications for the current conceptualization of genetic and environmental contributors of AN/atypical AN in twin and molecular genetic studies.ConclusionWe propose separating the starvation‐induced mechanisms relevant for AN/atypical AN development from the reasons/mechanisms inherent to weight loss.
Revealing the Demographic History of the European Nightjar (Caprimulgus europaeus)
Authors: Day George , Fox Graeme , Hipperson Helen , Maher Kathryn H. , Tucker Rachel , Horsburgh Gavin J. , Waters Dean , Durant Kate L. , Burke Terry , Slate Jon , Arnold Kathryn E. 
Published: October 2024

ABSTRACTA species' demographic history gives important context to contemporary population genetics and a possible insight into past responses to climate change; with an individual's genome providing a window into the evolutionary history of contemporary populations. Pairwise sequentially Markovian coalescent (PSMC) analysis uses information from a single genome to derive fluctuations in effective population size change over the last ~5 million years. Here, we apply PSMC analysis to two European nightjar (Caprimulgus europaeus) genomes, sampled in Northwest and Southern Europe, with the aim of revealing the demographic history of nightjar in Europe. We successfully reconstructed effective population size over the last 5 million years. Our analysis shows that in response to global climate change, the effective population size of nightjar broadly increased under stable warm periods and decreased during cooler spans and prolonged glacial periods. PSMC analysis on the pseudo‐diploid combination of the two genomes revealed fluctuations in gene flow between ancestral populations over time, with gene flow ceasing by the last‐glacial period. Our results are tentatively suggestive of divergence in the European nightjar population, with timings consistent with differentiation being driven by restriction to different refugia during periods of glaciation. Finally, our results suggest that migratory behaviour in nightjar likely evolved prior to the last‐glacial period, with long‐distance migration seemingly persisting throughout the Pleistocene. However, further genetic structure analysis of individuals from known breeding sites across the species' contemporary range is needed to understand the extent and origins of range‐wide differentiation in nightjar.
Evaluation of a Nanopore Sequencing Strategy on Bacterial Communities From Marine Sediments
Authors: Lemoinne Alice , Dirberg Guillaume , Georges Myriam , Robinet Tony 
Published: October 2024

ABSTRACTFollowing the development of high‐throughput DNA sequencers, environmental prokaryotic communities were usually described by metabarcoding on short markers of the 16S domain. Among third‐generation sequencers, that offered the possibility to sequence the full 16S domain, the portable MinION from Oxford Nanopore was undervalued for metabarcoding because of its relatively higher error rate per read. Here we illustrate the limits and benefits of Nanopore sequencing devices by comparing the prokaryotic community structure in a mock community and 52 sediment samples from mangrove sites, inferred from full‐length 16S long‐reads (16S‐FL, ca. 1.5 kbp) on a MinION device, with those inferred from partial 16S short‐reads (16S‐V4V5, ca. 0.4 kbp, 16S‐V4V5) on Illumina MiSeq. 16S‐V4V5 and 16S‐FL retrieved all the bacterial species from the mock, but Nanopore long‐reads overestimated their diversity (56 species vs. 15). Whether these supplementary OTUs were artefactual or not, they only accounted for ca. 10% of the reads. From the sediment samples, with a coverage‐based rarefaction of reads and after singletons filtering, Mantel and Procrustean tests of co‐inertia showed that bacterial community structures inferred from 16S‐V4V5 and 16S‐FL were significantly similar, showing both a comparable contrast between sites and a coherent sea‐land orientation within sites. In our dataset, 84.7% and 98.8% of the 16S‐V4V5 assigned reads were assigned strictly to the same species and genus, respectively, than those detected by 16S‐FL. 16S‐FL detected 92.2% of the 309 families and 87.7% of the 448 genera that were detected by the short 16S‐V4V5. 16S‐FL recorded 973 additional species and 392 genus not detected by 16S‐V4V5 (31.5% and 10.4% of the 16S‐FL reads, respectively, among which 67.8% and 79.3% were assigned), produced by both primer specificities and different error rates. Thus, our results concluded an overall similarity between 16S‐V4V5 and 16S‐FL sequencing strategies for this type of environmental samples.
Validation of Environmental DNA for Estimating Proportional and Absolute Biomass
Authors: Ledger Kimberly J. , Hicks Mary Beth Rew , Hurst Thomas P. , Larson Wes , Baetscher Diana S. 
Published: October 2024

ABSTRACTEnvironmental DNA (eDNA) has significant potential to improve the efficiency of biological sampling and detect species that pose challenges for traditional sampling methods. However, a key obstacle in utilizing eDNA data for ecosystem management is uncertainty surrounding the ability to estimate abundance or biomass of multiple species simultaneously. In this study, we use experimental trials with known biomasses of multiple species to explore the feasibility of (1) estimating species proportions from eDNA metabarcoding data and (2) estimating absolute eDNA concentrations of multiple species by scaling metabarcoding proportions with eDNA concentrations of a single species obtained from qPCR. The focal species for this study were three gadid fishes that are key components of marine ecosystems in Alaska and vary in their distribution and habitat use: Walleye pollock (Gadus chalcogrammus), Pacific cod (Gadus macrocephalus), and Arctic cod (Boreogadus saida). After designing gadid‐specific metabarcoding primers and accounting for PCR biases in the metabarcoding data, we found corrected read proportions closely approximated the true biomass proportions of species. Furthermore, we found strong positive relationships between absolute eDNA concentration and absolute biomass for Arctic cod and Pacific cod using quantitative metabarcoding data combined with estimates of Walleye pollock eDNA concentration derived from qPCR. These findings suggest that it is possible to accurately quantify species compositions and estimate metrics of biomass for gadids in real‐world scenarios. Furthermore, this work provides a framework for developing primers and analytical approaches that can be applied to other species to improve the quantitative utility of eDNA.
Impact of the adenosine receptor A2BR expressed on myeloid cells on immune regulation during pregnancy
Authors: Dietz Stefanie , Hebel Janine , Rühle Jessica , Huff Alisha , Eltzschig Holger K. , Lajqi Trim , Poets Christian F. , Gille Christian , Köstlin‐Gille Natascha 
Published: October 2024

AbstractDuring pregnancy, the maternal immune system must carefully balance protection against pathogens with tolerance toward the semiallogeneic fetus. Dysfunctions of the immune system can lead to severe complications such as preeclampsia, fetal growth restriction, or pregnancy loss. Adenosine plays a role in physiological processes and plasma‐level increase during pregnancy. The adenosine receptor A2B (A2BR), which is expressed on both, immune and nonimmune cells, is activated by high adenosine concentrations, achieved during pregnancy. We investigated the impact of A2BR expressed on myeloid cells on immune regulation during pregnancy using a mouse model with myeloid deficiency for A2BR. We demonstrate systemic changes in myeloid and lymphoid cell populations during pregnancy in A2BR‐KO (Adora2B923f/f‐LysMCre) mice with increased monocytes, neutrophils, and T cells but decreased B cells as well as altered T‐cell subpopulations with decreased Th1 cells and Tregs and increased Th17 cells. Lack of A2BR on myeloid cells caused an increased systemic expression of IL‐6 but decreased systemic accumulation and function of MDSC and reduced numbers of uterine natural killer cells. The pregnancy outcome was only marginally affected. Our results demonstrate that A2BR on myeloid cells plays a role in immune regulation during pregnancy, but the clinical impact on pregnancy remains unclear.
Making Sense of ‘Us’: Mechanisms Linking Attachment Avoidance and Couple Identity Clarity
Authors: Slotter Erica B. , Emery Lydia F. , Audigier Alexis , Gardner Wendi L. 
Published: October 2024

ABSTRACTJust as people strive to understand their own individual identities—to form a clear and coherent sense of who they are—they also seek to gain a clear understanding of who and what they are as a couple. However, some people may struggle in this goal; specifically, people high in attachment avoidance, who face barriers unique to the specific nature of their insecurity. We investigated whether attachment avoidance is negatively correlated with couple identity clarity and tested potential mechanisms for this association. We proposed that less interpersonal closeness and self‐verification from their partner would mediate the association between attachment avoidance and couple identity clarity. We found support for these hypotheses across three studies (total N = 912). Thus, attachment avoidance may create identity‐specific challenges in relationships.
Functional organic 7,7,8,8‐tetracyanoquinodimethane artificial layers for the dendrite suppressed lithium metal anodes
Authors: Liu Qing , Zheng Zhiyong , Xiong Peixun , Huang Chun , Huang Shengyang , Zhao Baohuai , Wu Yongan , Zhang Yi , Kim Bo‐Kyong , Yu Xu , Park Ho Seok 
Published: October 2024

AbstractThe large‐scale industrialization of lithium metal (Li), as a potential anode for a high energy density energy storage system, has been hindered by dendrite growth. The construction of an artificial solid electrolyte interphase layer featuring high ionic and low electronic conductivity has been verified to be a high‐performance strategy to confine the dendrite growth and promote the Li anode stability. Therefore, a functional organic protective layer is homogeneously deposited on the Li anode surface via an in situ chemical reaction between tetracyanoquinodimethane (TCNQ) and Li. The as‐synthesized Lin‐TCNQ organic film could efficiently trap non‐uniform Li deposition and restrain dendrite propagation. Particularly, an asymmetric M‐TCNQ‐Li|Cu cell with the Lin‐TCNQ layer breezed through a high Coulombic efficiency of 91.15% after 100 cycles at 1.0 mA cm−2. The M‐TCNQ‐Li|NCM622 cell delivered a high capacity of 143.40 mAh g−1 at 0.2 C and maintained a good cyclic stability of 110.44 mAh g−1 after 160 cycles. The analysis results of spectroscopic tests further demonstrate that the Lin‐TCNQ with the enhanced absorption energy is conducive to lithiophilicity and decreases the overpotential of Li deposition.
Two familial cases of infantile epileptic spasms syndrome associated with UDP‐glucose‐6‐dehydrogenase deficiency
Authors: Suyo C. , Reyes Valenzuela G. , Melgarejo S. , Loos M. , Juanes M. , Touzon M. S. , Angarita G. , Mesa M. , Alonso C. , Caraballo R. 
Published: October 2024

AbstractDevelopmental and epileptic encephalopathies (DEEs) are severe forms of epilepsy characterized by seizure onset in infancy or childhood. The seizures are typically drug‐resistant and often accompanied by significant alterations in the electroencephalogram (EEG). DEEs are associated with neurodevelopmental impairment, which can arise from both the epileptic activity itself and the underlying etiology, which is most often genetic in origin. We present the clinical and molecular features of two patients with DEE associated with a pathogenic variant in the UGDH gene. This gene encodes a protein that converts uridine diphosphate (UDP)‐glucose into UDP‐glucuronate, which plays a crucial role in the biosynthesis of glycosaminoglycans, essential components of the connective tissue and extracellular matrix. Both patients started with epileptic spasms associated with a pattern of hypsarrhythmia in the EEG at 4 months of age. Both developed global developmental delay and the physical examination revealed hypotonia and mildly dysmorphic features. In both families, there was another affected sibling with a similar clinical presentation, although genetic studies were not performed in one of these children. A homozygous pathogenic variant in the UGDH gene, NM_003359.4:c.131C>T – p.(Ala44Val), previously reported to be associated with the described phenotype, was identified.
Purging frequency and number of purging methods as indicators of severity in bulimia nervosa: Interactive effects with binge eating episodes
Authors: Dieffenbacher Anna L. , Meule Adrian , Voderholzer Ulrich 
Published: October 2024

AbstractObjectiveIn the Diagnostic and Statistical Manual of Mental Disorders‐5, severity of bulimia nervosa (BN) is defined by the frequency of purging behaviour. Previous research suggests the number of purging methods as an alternative rating. The current analysis investigated characteristics (sociodemographic and treatment‐related variables, body mass index [BMI], eating disorder‐specific and general psychopathology) of persons with BN as a function of purging frequency and number of purging methods in order to examine which approach might be better suited for indicating severity of BN.MethodTwo‐hundred and sixty‐one persons (98.5% female; mean age 25.2 years, SD = 9.41; mean BMI 22.0 kg/m2, SD = 3.79) with BN completed self‐report questionnaires on eating disorder‐specific and general psychopathology at admission to inpatient (n = 214) or daypatient (n = 47) treatment.ResultsHigher severity based on either purging frequency or number of purging methods tended to relate to lower BMI and higher eating disorder‐specific and general psychopathology. In addition, binge‐eating frequency differentially related to eating disorder‐specific and general psychopathology as a function of severity groups.ConclusionsThis study partially supports the utility of both purging frequency and the number of purging methods as indicators of severity in persons with BN. However, focussing only on purging behaviours may be short‐sighted as it appears necessary to consider the number of binge‐eating episodes as well when evaluating severity of BN.