bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Hydraulic Redistribution on Multispecies Vegetation Water Use in a Semiarid Savanna Ecosystem: An Experimental and Modeling Synthesis
Authors: Lee Esther , Kumar Praveen , Barron‐Gafford Greg A. , Hendryx Sean M. , Sanchez‐Cañete Enrique P. , Minor Rebecca L. , Colella Tony , Scott Russell L. 
Published: May 2018

AbstractA major challenge in critical zone science is to understand and predict the interaction between above‐ground and below‐ground ecohydrologic processes. One process that facilitates this connection is hydraulic redistribution, a phenomenon by which roots serve as preferential pathways for water movement from wet to dry soil layers. We use a multilayer canopy model in conjunction with experimental data to quantify the influence of hydraulic redistribution on ecohydrologic processes in order to characterize the competitive and facilitative interaction between mesquite trees and bunchgrasses in a semiarid savanna. Both measured and simulated results show that hydraulic descent dominates during the wet monsoon season, whereas hydraulic lift occurs between precipitation events. For 2015 year‐long simulation, we find about 17% of precipitation is absorbed as soil moisture, with the rest of the precipitation returning to the atmosphere as evapotranspiration. In the wet season, 13% of precipitation is transferred to deep soil (>1.5 m) through roots, and in the dry season, 9% of this redistributed water is then transported back to shallow soil depths (<0.5 m). Assuming water supplied through hydraulic redistribution is well‐mixed with moisture transported directly through the soil matrix and supports vegetation evapotranspiration, hydraulic redistribution supports 47% of mesquite transpiration and 9% of understory transpiration. Through modeling and experimental synthesis, this study demonstrates that in semiarid savanna ecosystems, mesquite exhibits a competitive advantage over understory bunchgrass through hydraulic redistribution. This analysis evaluates the relationship between two coexisting vegetation types that could be expanded to multiple vegetation species sharing resources in an ecosystem.
Effect of the Initial Shape of Coronal Mass Ejections on 3‐D MHD Simulations and Geoeffectiveness Predictions
Authors: Scolini C. , Verbeke C. , Poedts S. , Chané E. , Pomoell J. , Zuccarello F. P. 
Published: May 2018

AbstractCoronal mass ejections (CMEs) are the major space weather drivers, and an accurate modeling of their onset and propagation up to 1 AU represents a key issue for more reliable space weather forecasts. In this paper we use the newly developed EUropean Heliospheric FORecasting Information Asset (EUHFORIA) heliospheric model to test the effect of different CME shapes on simulation outputs. In particular, we investigate the notion of “spherical” CME shape, with the aim of bringing to the attention of the space weather community the great implications of the CME shape implementation details for simulation results and geoeffectiveness predictions. We take as case study an artificial Earth‐directed CME launched on 6 June 2008, corresponding to a period of quiet solar wind conditions near Earth. We discuss the implementation of the cone model used to inject the CME into the modeled ambient solar wind, running several simulations of the event and investigating the outputs in interplanetary space and at different spacecraft and planetary locations. We apply empirical relations to simulation outputs at L1 to estimate the expected CME geoeffectiveness in terms of the magnetopause stand‐off distance and the induced Kp index. Our analysis shows that talking about spherical CMEs is ambiguous unless one has detailed information on the implementation of the CME shape in the model. All the parameters specifying the CME shape in the model significantly affect simulation results at 1 AU as well as the predicted CME geoeffectiveness, confirming the pivotal role played by the shape implementation details in space weather forecasts.
An Empirical Model of Vertical Plasma Drift Over the African Sector
Authors: Dubazane Makhosonke Berthwell , Habarulema John Bosco 
Published: May 2018

AbstractDaytime vertical E × B drift can be derived from difference of H‐component magnetic field measurements (ΔH) using a pair of low‐latitude and equatorial latitude magnetometer stations. Knowledge of E × B drift is of utmost importance in space weather‐related predictions since it can significantly affect ionospheric density structures and dynamics. For the first time, we developed a quantitative relationship between equatorial electrojet (ΔH) and vertical E × B drift over the African region using magnetometer data and E × B drift observations from the Communication and Navigation Outage Forecasting System (C/NOFS) satellite during local daytime (0700–1800 LT) from 2008 to 2013 when magnetometer data were available. Additionally, we have constructed vertical E × B drift models over the African sector based on C/NOFS vertical E × B drift data and relevant physical and geophysical inputs. Validating using data not included in model development, our model estimated C/NOFS vertical E × B drift with correlation coefficient values of 0.85 and 0.56 during quiet and disturbed conditions, respectively. The daily pattern of the E × B drift velocity is consistent between the developed model based on C/NOFS data and the climatological Scherliess‐Fejer model. However, the Scherliess‐Fejer model generally overestimated C/NOFS vertical E × B drift (in most cases) with a correlation coefficient of 0.54 during quiet conditions.
Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro
Authors: Hakki Sema S. , Bozkurt S. Buket , Türkay Emre , Dard Michel , Purali Nuhan , Götz Werner 
Published: May 2018

AbstractAmelogenin (AMG) is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG (rhAMG) on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts (OCCM-30) were treated with different concentrations (0.1, 1, 10, 100, 1000, 10,000, 100,000 ng · mL-1) of recombinant human AMG (rhAMG) and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein (BSP), osteocalcin (OCN), collagen type I (COL I), osteopontin (OPN), runt-related transcription factor 2 (Runx2), cementum attachment protein (CAP), and alkaline phosphatase (ALP) genes using quantitative RT-PCR. The dose response of rhAMG was evaluated using a real-time cell analyzer. Total RNA was isolated on day 3, and cell mineralization was assessed using von Kossa staining on day 8. COL I, OPN and lysosomal-associated membrane protein-1 (LAMP-1), which is a cell surface binding site for amelogenin, were evaluated using immunocytochemistry. F-actin bundles were imaged using confocal microscopy. rhAMG at a concentration of 100,000 ng · mL-1 increased cell proliferation after 72 h compared to the other concentrations and the untreated control group. rhAMG (100,000 ng · mL-1) upregulated BSP and OCN mRNA expression levels eightfold and fivefold, respectively. rhAMG at a concentration of 100,000 ng · mL-1 remarkably enhanced LAMP-1 staining in cementoblasts. Increased numbers of mineralized nodules were observed at concentrations of 10,000 and 100,000 ng · mL-1 rhAMG. The present data suggest that rhAMG is a potent regulator of gene expression in cementoblasts and support the potential application of rhAMG in therapies aimed at fast regeneration of damaged periodontal tissue.
p53-dependent CD51 expression contributes to characteristics of cancer stem cells in prostate cancer
Authors: Sui Xin , Cai Jianye , Li Hongyu , He Chenchen , Zhou Congya , Dong Yiping , Chen Li , Zhang Bin , Wang Yingnan , Zhang Yanan , Qiu Yuan , Zhang Yuanyuan , Zhao Yang , Huang Yinong , Shen Yutian , Wu Haoxiang , Xiao Jiaqi , Mason Clifford , Zhu Qing , Han Suxia 
Published: May 2018

Abstract
Castration-resistant prostate cancer (CRPC), which is considered to contain cancer stem cells (CSCs), leads to a high relapse rate in patients with prostate cancer (PCa). However, the markers of prostate CSCs are controversial. Here we demonstrate that CD51, in part, correlates with the poor prognosis of PCa patients. Further, we find that CD51 is a functional molecule that is able to promote the malignancy of PCa through enhancing tumor initiation, metastatic potential, and chemoresistance. Moreover, we find that elevated CD51 expression in PCa specimens correlates with p53 loss of function. Mechanistically, we demonstrate that p53 acts via Sp1/3 to repress CD51 transcription, and CD51 is required for PCa stemness and metastasis properties, and is downregulated by p53. Taken together, these results indicate that CD51 is a novel functional marker for PCa, which may provide a therapeutic target for the efficiently restricting PCa progression.
E2F1 inhibition mediates cell death of metastatic melanoma
Authors: Rouaud Florian , Hamouda-Tekaya Nedra , Cerezo Michaël , Abbe Patricia , Zangari Joséphine , Hofman Veronique , Ohanna Mickaël , Mograbi Baharia , El-Hachem Najla , Benfodda Zohra , Lebeau Alexandre , Tulic Meri K. , Hofman Paul , Bertolotto Corine , Passeron Thierry , Annicotte Jean-Sébastien , Ballotti Robert , Rocchi Stéphane 
Published: May 2018

AbstractMelanoma is one of the most lethal cancers when it reaches a metastatic stage. Despite advancements in targeted therapies (BRAF inhibitors) or immunotherapies (anti-CTLA-4 or anti-PD1), most patients with melanoma will need additional treatment. Thus, there is an urgent need to develop new therapeutical approaches to bypass resistance and achieve more prolonged responses. In this context, we were interested in E2F1, a transcription factor that plays a major role in the control of cell cycle under physiological and pathological conditions. Here we confirmed that E2F1 is highly expressed in melanoma cells. Inhibition of E2F1 activity further increased melanoma cell death and senescence, both in vitro and in vivo. Moreover, blocking E2F1 also induced death of melanoma cells resistant to BRAF inhibitors. In conclusion, our studies suggest that targeting the E2F1 signaling pathway may be therapeutically relevant for melanoma.
Molecular functional analyses revealed essential roles of HSP90 and lamin A/C in growth, migration, and self-aggregation of dermal papilla cells
Authors: Thanomkitti Kanchalit , Fong-ngern Kedsarin , Sueksakit Kanyarat , Thuangtong Rattapon , Thongboonkerd Visith 
Published: May 2018

AbstractPrevious expression study using quantitative proteomics has shown that immune-mediated pathway may not be the main mechanism inducing alopecia areata (AA). Nevertheless, functional impact of such expression data set remained unknown and unexplored. This study thus aimed to define potentially novel mechanisms of the AA pathogenesis by functional investigations of the differentially expressed proteins previously identified from lesional biopsies. From 122 altered proteins, protein–protein interactions network analysis revealed that downregulated heat shock protein 90 (HSP90) and lamin A/C served as the central nodes of protein–protein interactions involving in several crucial biological functions, including cytoskeleton organization, extracellular matrix organization, and tissue development. Interaction between HSP90 and lamin A/C in dermal papilla cells (DPCs) was confirmed by reciprocal immunoprecipitation and immunofluorescence co-staining. Small-interfering RNA (siRNA) targeting to HSP90 (siHSP90) and lamin A/C (siLamin A/C) effectively reduced levels of HSP90 and lamin A/C, respectively and vice versa, comparing to non-transfected and siControl-transfected cells, strengthening their interactive roles in DPCs. Functional investigations revealed that DPCs transfected with siHSP90 and siLamin A/C had defective cell proliferation and growth, prolonged doubling time, cell cycle arrest at G0/G1 phase, and defective self-aggregation formation. Moreover, siHSP90-transfected cells had less spindle index, reduced levels of vimentin (mesenchymal marker) and fibronectin (extracellular matrix), and defective migratory activity. Our data have demonstrated for the first time that HSP90 and lamin A/C physically interact with each other. Moreover, both of them are essential for growth, migration, and self-aggregation of DPCs and can be linked to the disease mechanisms of AA.
Programmable sequential mutagenesis by inducible Cpf1 crRNA array inversion
Authors: Chow Ryan D. , Kim Hyunu Ray , Chen Sidi 
Published: May 2018

AbstractMutations and genetic alterations are often sequentially acquired in various biological and pathological processes, such as development, evolution, and cancer. Certain phenotypes only manifest with precise temporal sequences of genetic events. While multiple approaches have been developed to model the effects of mutations in tumorigenesis, few recapitulate the stepwise nature of cancer evolution. Here we describe a flexible sequential mutagenesis system, Cpf1-Flip, with inducible inversion of a single crRNA array (FlipArray), and demonstrate its application in stepwise mutagenesis in murine and human cells. As a proof-of-concept, we further utilize Cpf1-Flip in a pooled-library approach to model the acquisition of diverse resistance mutations to cancer immunotherapy. Cpf1-Flip offers a simple, versatile, and controlled approach for precise mutagenesis of multiple loci in a sequential manner.
Quantifying climate feedbacks in polar regions
Authors: Goosse Hugues , Kay Jennifer E. , Armour Kyle C. , Bodas-Salcedo Alejandro , Chepfer Helene , Docquier David , Jonko Alexandra , Kushner Paul J. , Lecomte Olivier , Massonnet François , Park Hyo-Seok , Pithan Felix , Svensson Gunilla , Vancoppenolle Martin 
Published: May 2018

AbstractThe concept of feedback is key in assessing whether a perturbation to a system is amplified or damped by mechanisms internal to the system. In polar regions, climate dynamics are controlled by both radiative and non-radiative interactions between the atmosphere, ocean, sea ice, ice sheets and land surfaces. Precisely quantifying polar feedbacks is required for a process-oriented evaluation of climate models, a clear understanding of the processes responsible for polar climate changes, and a reduction in uncertainty associated with model projections. This quantification can be performed using a simple and consistent approach that is valid for a wide range of feedbacks, offering the opportunity for more systematic feedback analyses and a better understanding of polar climate changes.
Hematopoietic stem cells can differentiate into restricted myeloid progenitors before cell division in mice
Authors: Grinenko Tatyana , Eugster Anne , Thielecke Lars , Ramasz Beáta , Krüger Anja , Dietz Sevina , Glauche Ingmar , Gerbaulet Alexander , von Bonin Malte , Basak Onur , Clevers Hans , Chavakis Triantafyllos , Wielockx Ben 
Published: May 2018

AbstractHematopoietic stem cells (HSCs) continuously replenish all blood cell types through a series of differentiation steps and repeated cell divisions that involve the generation of lineage-committed progenitors. However, whether cell division in HSCs precedes differentiation is unclear. To this end, we used an HSC cell-tracing approach and Ki67RFP knock-in mice, in a non-conditioned transplantation model, to assess divisional history, cell cycle progression, and differentiation of adult HSCs. Our results reveal that HSCs are able to differentiate into restricted progenitors, especially common myeloid, megakaryocyte-erythroid and pre-megakaryocyte progenitors, without undergoing cell division and even before entering the S phase of the cell cycle. Additionally, the phenotype of the undivided but differentiated progenitors correlated with the expression of lineage-specific genes and loss of multipotency. Thus HSC fate decisions can be uncoupled from physical cell division. These results facilitate a better understanding of the mechanisms that control fate decisions in hematopoietic cells.
DOT1L inhibition attenuates graft-versus-host disease by allogeneic T cells in adoptive immunotherapy models
Authors: Kagoya Yuki , Nakatsugawa Munehide , Saso Kayoko , Guo Tingxi , Anczurowski Mark , Wang Chung-Hsi , Butler Marcus O. , Arrowsmith Cheryl H. , Hirano Naoto 
Published: May 2018

AbstractAdoptive T-cell therapy is a promising therapeutic approach for cancer patients. The use of allogeneic T-cell grafts will improve its applicability and versatility provided that inherent allogeneic responses are controlled. T-cell activation is finely regulated by multiple signaling molecules that are transcriptionally controlled by epigenetic mechanisms. Here we report that inhibiting DOT1L, a histone H3-lysine 79 methyltransferase, alleviates allogeneic T-cell responses. DOT1L inhibition reduces miR-181a expression, which in turn increases the ERK phosphatase DUSP6 expression and selectively ameliorates low-avidity T-cell responses through globally suppressing T-cell activation-induced gene expression alterations. The inhibition of DOT1L or DUSP6 overexpression in T cells attenuates the development of graft-versus-host disease, while retaining potent antitumor activity in xenogeneic and allogeneic adoptive immunotherapy models. These results suggest that DOT1L inhibition may enable the safe and effective use of allogeneic antitumor T cells by suppressing unwanted immunological reactions in adoptive immunotherapy.
Early Cambrian origin of the shelf sediment mixed layer
Authors: Gougeon Romain C. , Mángano M. Gabriela , Buatois Luis A. , Narbonne Guy M. , Laing Brittany A. 
Published: May 2018

AbstractThe mixed layer of modern oceans is a zone of fully homogenized sediment resulting from bioturbation. The mixed layer is host to complex biogeochemical cycles that directly impact ecosystem functioning, affecting ocean productivity and marine biodiversity. The timing of origin of the mixed layer has been controversial, with estimates ranging from Cambrian to Silurian, hindering our understanding of biogeochemical cycling and ecosystem dynamics in deep time. Here we report evidence from the Global Stratotype Section and Point (GSSP) of the basal Cambrian in the Burin Peninsula of Newfoundland, Canada, showing that a well-developed mixed layer of similar structure to that of modern marine sediments was established in shallow marine settings by the early Cambrian (approximately 529 million years ago). These findings imply that the benthos significantly contributed to establishing new biogeochemical cycles during the Cambrian explosion.
Hybrid nanostructured particles via surfactant-free double miniemulsion polymerization
Authors: Zhao Yongliang , Liu Junli , Chen Zhi , Zhu Xiaomin , Möller Martin 
Published: May 2018

AbstractDouble emulsions are complex fluid systems, in which droplets of a dispersed liquid phase contain even smaller dispersed liquid droplets. Particularly, water-in-oil-in-water double emulsions provide significant advantages over simple oil-in-water emulsions for microencapsulation, such as carrier of both aqueous and oily payloads and sustained release profile. However, double emulsions are thermodynamically unstable systems consisting typically of relatively large droplets. Here we show that nanoscale water-in-oil-in-water double emulsions can be prepared by adding a silica precursor polymer, hyperbranched polyethoxysiloxane, to the oil phase without any additional surfactants. The resulting double miniemulsions are transformed to robust water@SiO2@polymer@SiO2nanocapsules via conversion of the precursor to silica and polymerization of the oil phase. Other intriguing nanostructures like nanorattles and Janus-like nanomushrooms can also be obtained by changing preparation conditions. This simple surfactant-free double miniemulsion polymerization technique opens a promising avenue for mass production of various complex hybrid nanostructures that are amenable to numerous applications.
Dynamics of a qubit while simultaneously monitoring its relaxation and dephasing
Authors: Ficheux Q. , Jezouin S. , Leghtas Z. , Huard B. 
Published: May 2018

AbstractDecoherence originates from the leakage of quantum information into external degrees of freedom. For a qubit, the two main decoherence channels are relaxation and dephasing. Here, we report an experiment on a superconducting qubit where we retrieve part of the lost information in both of these channels. We demonstrate that raw averaging the corresponding measurement records provides a full quantum tomography of the qubit state where all three components of the effective spin-1/2 are simultaneously measured. From single realizations of the experiment, it is possible to infer the quantum trajectories followed by the qubit state conditioned on relaxation and/or dephasing channels. The incompatibility between these quantum measurements of the qubit leads to observable consequences in the statistics of quantum states. The high level of controllability of superconducting circuits enables us to explore many regimes from the Zeno effect to underdamped Rabi oscillations depending on the relative strengths of driving, dephasing, and relaxation.
Iron-specific Signal Separation from within Heavy Metal Stained Biological Samples Using X-Ray Microtomography with Polychromatic Source and Energy-Integrating Detectors
Authors: Katchalski Tsvi , Case Tom , Kim Keun-young , Ramachandra Ranjan , Bushong Eric A. , Deerinck Thomas J. , Haberl Matthias G. , Mackey Mason R. , Peltier Steven , Castillon Guillaume A. , Fujikawa Nobuko , Lawrence Albert R. , Ellisman Mark H. 
Published: May 2018

AbstractBiological samples are frequently stained with heavy metals in preparation for examining the macro, micro and ultra-structure using X-ray microtomography and electron microscopy. A single X-ray microtomography scan reveals detailed 3D structure based on staining density, yet it lacks both material composition and functional information. Using a commercially available polychromatic X-ray source, energy integrating detectors and a two-scan configuration labelled by their energy- “High” and “Low”, we demonstrate how a specific element, here shown with iron, can be detected from a mixture with other heavy metals. With proper selection of scan configuration, achieving strong overlap of source characteristic emission lines and iron K-edge absorption, iron absorption was enhanced enabling K-edge imaging. Specifically, iron images were obtained by scatter plot material analysis, after selecting specific regions within scatter plots generated from the “High” and “Low” scans. Using this method, we identified iron rich regions associated with an iron staining reaction that marks the nodes of Ranvier along nerve axons within mouse spinal roots, also stained with osmium metal commonly used for electron microscopy.