bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultrahigh‐cell‐density heterotrophic cultivation of the unicellular green microalga Scenedesmus acuminatus and application of the cells to photoautotrophic culture enhance biomass and lipid production
Authors: Jin Hu , Zhang Hu , Zhou Zhiwei , Li Kunpeng , Hou Guoli , Xu Quan , Chuai Wenhua , Zhang Chengwu , Han Danxiang , Hu Qiang 
Published: October 2019

AbstractAlthough production of biodiesels from microalgae is proved to be technically feasible, a commercially viable system has yet to emerge. High‐cell‐density fermentation of microalgae can be coupled with photoautotrophic cultivation to produce oils. In this study, by optimizing culturing conditions and employing a sophisticated substrate feed control strategy, ultrahigh‐cell‐density of 286 and 283.5 g/L was achieved for the unicellular alga Scenedesmus acuminatus grown in 7.5‐L bench‐scale and 1,000‐L pilot‐scale fermenters, respectively. The outdoor scale‐up experiments indicated that heterotrophically grown S. acuminatus cells are more productive in terms of both biomass and lipid accumulation when they are inoculated in photobioreactors for lipid production as compared to the cells originally grown under photoautotrophic conditions. Technoeconomic analysis based on the pilot‐scale data indicated that the cost of heterotrophic cultivation of microalgae for biomass production is comparable with that of the open‐pond system and much lower than that of tubular PBR, if the biomass yield was higher than 200 g/L. This study demonstrated the economic viability of heterotrophic cultivation on large‐scale microalgal inocula production, but ultrahigh‐productivity fermentation is a prerequisite. Moreover, the advantages of the combined heterotrophic and photoautotrophic cultivation of microalgae for biofuels production were also verified in the pilot‐scale.
Cellular localization of cytochrome bd in cyanobacteria using genetic code expansion
Authors: Cohen Mor , Ozer Eden , Kushmaro Ariel , Alfonta Lital 
Published: October 2019

AbstractPhotosynthesis is one of the most fundamental and complex mechanisms in nature. It is a well‐studied process, however, some photosynthetic mechanisms are yet to be deciphered. One of the many proteins that take part in photosynthesis, cytochrome bd, is a terminal oxidase protein that plays a role both in photosynthesis and in respiration in various organisms, specifically, in cyanobacteria. To clarify the role of cytochrome bd in cyanobacteria, a system for the incorporation of an unnatural amino acid into a genomic membrane protein cytochrome bd was constructed in Synechococcus sp. PCC7942. N‐propargyl‐
l‐lysine (PrK) was incorporated into mutants of cytochrome bd. Incorporation was verified and the functionality of the mutant cytochrome bd was tested, revealing that both electrochemical and biochemical activities were relatively similar to those of the wild‐type protein. The incorporation of PrK was followed by a highly specific labeling and localization of the protein. PrK that was incorporated into the protein enabled a “click” reaction in a bio‐orthogonal manner through its alkyne group in a highly specific manner. Cytochrome bd was found to be localized mostly in thylakoid membranes, as was confirmed by an enzyme‐linked immunosorbent assay, indicating that our developed localization method is reliable and can be further used to label endogenous proteins in cyanobacteria.
Survival of aging CD264+ and CD264− populations of human bone marrow mesenchymal stem cells is independent of colony‐forming efficiency
Authors: Madsen Sean D. , Jones Sean H. , Tucker H. Alan , Giler Margaret K. , Muller Dyllan C. , Discher Carson T. , Russell Katie C. , Dobek Georgina L. , Sammarco Mimi C. , Bunnell Bruce A. , O'Connor Kim C. 
Published: October 2019

AbstractIn vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unknown. Here, we employ a biomarker of cellular aging, the decoy TRAIL receptor CD264, to compare the survival kinetics of two cell populations in human bone marrow MSC (hBM‐MSC) cultures. Sorted CD264+ hBM‐MSCs from two age‐matched donors have elevated β‐galactosidase activity, decreased differentiation potential and form in vitro colonies inefficiently relative to CD264− hBM‐MSCs. Counterintuitive to their aging phenotype, CD264+ hBM‐MSCs exhibited comparable survival to matched CD264− hBM‐MSCs from the same culture during in vitro colony formation and in vivo when implanted ectopically in immunodeficient NIH III mice. In vitro and in vivo survival of these two cell populations were independent of colony‐forming efficiency. These findings have ramifications for the preparation of hBM‐MSC therapies given the prevalence of aging CD264+ cells in hBM‐MSC cultures and the popularity of colony‐forming efficiency as a quality control metric in preclinical and clinical studies with MSCs.
Purification and Kinetic Characterization of the Essential Condensation Enzymes Involved in Prodiginine and Tambjamine Biosynthesis
Authors: Picott Katherine J. , Deichert Julie A. , deKemp Ella M. , Snieckus Victor , Ross Avena C. 
Published: October 2019

AbstractProdiginines and tambjamines are related families of bioactive alkaloid natural products with pharmaceutical potential. Both compound families result from a convergent biosynthetic pathway ending in the condensation of a conserved bipyrrole core with a variable partner. This reaction is performed by unique condensation enzymes, and has the potential to be manipulated to produce new pyrrolic compounds. We have purified and reconstituted the in vitro activity of the condensation enzymes PigC and TamQ from Pseudoalteromonas sp., which are involved, respectively, in the prodiginine and tambjamine biosynthetic pathways. Kinetic analysis confirmed a Uni Uni Bi Uni ping‐pong reaction sequence with competitive and uncompetitive substrate inhibition for PigC and TamQ respectively. The kinetic parameters of each enzyme provide insight into their differing substrate scope, and suggest that TamQ may have evolved a wide substrate tolerance that can be used for the production of novel prodiginines and tambjamines.
Review on Photogenerated Hole Modulation Strategies in Photoelectrocatalysis for Solar Fuel Production
Authors: Sun Rui , Zhang Ziqing , Li Zhijun , Jing Liqiang 
Published: October 2019

AbstractPhotoelectrocatalytic (PEC) technique provides a clean, low‐cost, and environmental‐friendly route with great potential for addressing energy crisis mainly through water splitting and CO2 reduction by utilizing solar energy. In general, it is favorable for the added external electric field to greatly modulate the photogenerated electrons in PEC process. However, it is a rate‐determining step as another half‐reaction for the photogenerated hole to induce water oxidation since it is usually involved with 4 electrons for O2 evolution. Thus, it is highly desired to develop feasible strategies to modulate photogenerated hole for efficient solar‐to‐energy conversion. Unfortunately, it is frequently neglected compared to the electron regulation. Obviously, it is much meaningful to develop new efficient strategies for photogenerated hole modulation, along with to review recent developments. Herein, this minireview summarizes the recent developments of photoexcited hole modulation for PEC process to nanosized semiconductors, and it is highlighted for the strategies successfully developed in our group in recent years, mainly including surface polarization by modifying inorganic acids and halogen anions, and non‐metallic element doping. Moreover, related conclusions and several outlooks are put forward.
Synthesis of Ni−MoSx/g‐C3N4 for Photocatalytic Hydrogen Evolution under Visible Light
Authors: Wang Bo , Li Xia , Wu Haining , Xu Guangqing , Zhang Xinyi , Shu Xia , Lv Jun , Wu Yucheng 
Published: October 2019

AbstractGraphitic carbon nitride loaded with MoSx nanodots (MoSx/g‐C3N4) was synthesized through a sonochemical method. MoSx nanodots (3∼20 nm) are shown to have the same excellent co‐catalytic H2 evolution performance as that of Pt. Nevertheless, the photocatalytic activity of MoSx/g‐C3N4 decreased with time. To overcome this instability, we show that co‐incorporation of Ni can significantly reduce the photo‐corrosion. The rate of H2 evolution of Ni−MoSx/g‐C3N4 photocatalyst reaches a maximum of 5968 μmol h−1 g−1, which is 89 % higher than that of Pt/g‐C3N4. The element mapping images show that Ni is deposited on the surface of MoSx. The co‐presence of Ni and MoSx not only enhance optical light absorption by g‐C3N4, but also significantly improve the separation efficiency of photocarriers and thus the photocatalytic hydrogen evolution efficiency.
The Capacitive Behavior of Polyluminol on Carbon Nanotubes Electrodes
Authors: N'Diaye Jeanne , Hyun Chang Jin , Lian Keryn 
Published: October 2019

AbstractA polymerized luminol carbon nanotube (CNT) composite electrode was developed via an in situ chemical polymerization (CpLum) process. Density functional theory (DFT) simulation suggested the luminol molecules were preferentially aligned flat on the CNTs. This was further demonstrated through a morphological study, which showed the CpLum wrapped around each CNT homogeneously with an average thickness of 4.5±1.5 nm. The surface chemical analysis by X‐ray photoelectron spectroscopy (XPS) revealed a progressive increase in the nitrogen content and stabilized at 9 %. Deconvolution of the high‐resolution N 1s spectra suggested the presence of secondary and tertiary amine functional groups, which are the signatures of polymerized luminol. The composite electrodes exhibited a pseudocapacitive‐like behavior with 3.5 times increase in charge storage. The contributions from the CpLum coating and CNT substrate were differentiated and were further deconvoluted to quantify the capacitive charge storage of each component. The thin CpLum coating contributed 70 % of the total charge storage through pseudocapacitance. CpLum‐CNT electrodes also showed a high rate capability and good cycling stability, very promising for electrochemical capacitors.
Hole Migration in Telomere‐Based Oligonucleotide Anions and G‐Quadruplexes
Authors: Li Wen , Mjekiqi Edita , Douma Wessel , Wang Xin , Kavatsyuk Oksana , Hoekstra Ronnie , Poully Jean‐Christophe , Schlathölter Thomas 
Published: October 2019

AbstractVacuum ultraviolet photoionization of a gas‐phase oligonucleotide anion leads to the formation of a valence hole. This hole migrates towards an energetically favorable site where it can weaken bonds and ultimately lead to bond cleavage. We have studied Vacuum UV photoionization of deprotonated oligonucleotides containing the human telomere sequence dTTAGGG and G‐quadruplex structures consisting of four dTGGGGT single strands, stabilized by NH4+ counter ions. The oligonucleotide and G‐quadruplex anions were confined in a radiofrequency ion trap, interfaced with a synchrotron beamline and the photofragmentation was studied using time‐of‐flight mass spectrometry. Oligonucleotide 12‐mers containing the 5'‐TTAGGG sequence were found to predominantly break in the GGG region, whereas no selective bond cleavage region was observed for the reversed 5'‐GGGATT sequence. For G‐quadruplex structures, fragmentation was quenched and mostly non‐dissociative single and double electron removal was observed.
Annulation of Oxime‐Ether Tethered Donor–Acceptor Cyclopropanes
Authors: Irwin Lauren C. , Allen Meredith A. , Vriesen Matthew R. , Kerr Michael A. 
Published: October 2019

AbstractNovel oxime‐ether tethered cyclopropanes, when exposed to Yb(OTf)3 and heat, annulate to generate hydropyrrolo‐oxazines products that can be taken to their respective pyrrolidines via hydrogenative N−O bond cleavage. The hydropyrrolo‐oxazines are generated in a diastereoselective manner isolating the cis or trans product based on the temperature of the reaction. 20 examples of selective cis and trans hydropyrrolo‐oxazines were generated in high yields by temperature control.
Are parental changes related to improvements in preschool children's disruptive behaviours?
Authors: van der Veen‐Mulders Lianne , Hoekstra Pieter J. , Nauta Maaike H. , van den Hoofdakker Barbara J. 
Published: October 2019

AbstractObjectiveTo investigate whether changes in parenting after behavioural parent training in routine clinical care are associated with improvements in preschool children's disruptive behaviours.MethodWe evaluated changes after parent training in maternal and paternal self‐reports of parental discipline practices parenting sense of competence, and parents' ratings of child disruptive behaviours in parents of 63 children, with a one group pretest–posttest design. We also compared parenting parameters in this clinical sample with a nonclinical sample (n = 121).ResultsMothers' self‐reports of parental discipline practices and parenting sense of competence significantly improved after behavioural parent training. Less over‐reactivity in both mothers and fathers was associated with fewer disruptive behaviours in children. After parent training, mothers' ratings of their discipline techniques did not differ anymore from those in the nonclinical sample.ConclusionPositive changes in parental discipline practices, particularly less over‐reactive parental behaviours, were related to a decrease of disruptive child behaviours.
Proteomics comparison of exosomes from serum and plasma between ultracentrifugation and polymer‐based precipitation kit methods
Authors: Cao Feng , Gao Ying , Chu Qiao , Wu Qi , Zhao Lin , Lan Tao , Zhao Liang 
Published: October 2019

AbstractExosomes are vesicles with sizes ranging from 30 to 150 nm. The analysis and detection of blood exosomes offers an effective route for cancer diagnosis, prognosis assessment, and therapeutic evaluation of diseases. Due to the difference in separation procedure, collection method and the usage of anticoagulants, serum and plasma samples show diversity test results. In order to evaluate the isolation effect of exosomes in serum and plasma samples, two commonly used exosomal isolation methods, ultracentrifugation and polymer‐based precipitation kit, were used, respectively. And the isolation effects were evaluated by comparing the composition and abundant of proteins from isolated exosomes based on MS‐based proteomics analysis. The results showed that the plasma exosomes extracted by ultracentrifugation identified more exosome biomarkers, and the concentrations of these biomarkers were higher than others. And plasma exosomes could be a better sample for blood‐based proteomics research of exosomes. It would be more useful for future targeted biomarker discovery.
Analysis of impeller blade parameters and tip clearance of turboexpander in organic Rankine cycle system
Authors: Jia Wenguang , Wang Chuanwei , Zhang Kerui , Feng Shaohua , Yan Jinglu , Liu Bingcheng 
Published: October 2019

AbstractThe optimization of a turboexpander can significantly improve the performance of the organic Rankine cycle (ORC) system, which can use in low‐temperature geothermal energy for high‐efficiency power generation. Therefore, this paper studies and optimizes the expander in the ORC of low‐temperature geothermal energy around 90°C. Firstly, the influence of impeller parameters on the performance of the expander is analyzed. Then, the grid is divided by the turbine mesh, and numerical simulation is performed by CFX. The gas state equation selects the Peng–Robinson equation, and the turbulence equation selects the SST model. Finally, the effect of tip clearance on the performance of the expander was studied. Research on impeller blade parameters shows that with the increase in the outer diameter of the impeller blade outlet, the isentropic efficiency of the expander decreases, and the output power increases. As the increase in the angle between the direction of the impeller blade and the meridian plane, the outlet velocity of the blade increases, the temperature decreases, and the efficiency and control of the turboexpander will decrease. The meridional section width has few effects on the performance of the expander. The study of the tip clearance by numerical simulation shows that the existence of tip clearance will cause clearance flow between pressure surface and suction surface that interferes with the mainstream direction. When the tip clearance increases from 0 mm to 1.2 mm, the efficiency of the expander is reduced by 8.9%.
miR‐146a interacting with lncRNA EPB41L4A‐AS1 and lncRNA SNHG7 inhibits proliferation of bone marrow‐derived mesenchymal stem cells
Authors: Cui Penglei , Zhao Xiaoying , Liu Jinlong , Chen Xiaodong , Gao Yuan , Tao Kun , Wang Chuandong , Zhang Xiaoling 
Published: October 2019

AbstractEmerging evidence suggests that microRNA plays a pivotal role in cell proliferation. Our previous research has certified that miR‐146a attenuates osteoarthritis through the regulation of cartilage homeostasis. However, little information about the function of miR‐146a in bone marrow‐derived mesenchymal stem cells (BMSCs) proliferation and the underlying mechanism was available. Therefore, this study aims at investigating the role of miR‐146a on the proliferation of BMSCs and the possible mechanisms involved. The function of miR‐146a on BMSCs proliferation was studied through overexpression and knockdown of miR‐146a or the indicated long noncoding RNAs (lncRNAs) in BMSCs and then the proliferation rate of the BMSCs were detected by Cell Counting Kit‐8 assay, colony formation assay. Besides, flow cytometry was used to test the cell cycle state of BMSCs modified by overexpression or knockdown of miR‐146a or lncRNA EPB41L4A‐AS1 (EPB41L4A Antisense RNA 1) and small nucleolar RNA host gene 7 (SNHG7). The expression level of marker genes involved in modulating cell proliferation was evaluated by quantitative polymerase chain reaction and western blot analysis. We discovered that the knockdown of miR‐146a significantly promoted BMSCs proliferation. Moreover, miR‐146a could bind to and inhibit endogenous expression of EPB41L4A‐AS1 and SNHG7. Further study demonstrated that overexpression of EPB41L4A‐AS1 and SNHG7 significantly enhanced proliferation of BMSCs. For the first time, we certified that miR‐146a suppressed BMSCs proliferation, but EPB41L4A‐AS1 and SNHG7 promoted BMSCs proliferation in the present study. Mechanistically, miR‐146a significantly inhibited BMSCs proliferation partly through miR‐146a/EPB41L4A‐AS1 SNHG7/cell proliferation signaling pathway axis.
NDRG2andTLR7as novel DNA methylation prognostic signatures for acute myelocytic leukemia
Authors: Ge Fei , Zhang Ping , Niu Junwei , Pei Xiaohang , Lian Cheng , Yu Runhong , Ma Rongjun , Zhang Yin , Zhu Zunmin , Sun Kai 
Published: October 2019

AbstractAcute myelocytic leukemia (AML) is an aggressive malignant tumor and typically fatal without treatment. Identification and development of novel biomarkers could be beneficial for the diagnosis and prognosis of AML patients. Here, we aimed to identify the accurate DNA methylation prognostic signatures for AML patients. The DNA methylation data of AML patients and corresponding clinical information were retrieved from The Cancer Genome Atlas database. CPG sites that correlates closely with the survival of the AML patients were identified and further combined into CPG sites pairs to screen the survival‐related pairs. The prognostic signatures were identified by the C‐index and forward search algorithms and validated by the verification group. Finally, the functional enrichment analysis was performed on these CPG sites. As a result, a total of 498 CPG sites associated with the overall survival of AML patients was obtained. A prognostic signature composed of 10 CPG sites pairs was obtained and validated. The functional enrichment analysis showed prognostic genes were mainly enriched in tumor protein processing, cell differentiation, blood leukocyte immunity, and platelet growth factor pathways. In summary, we identified two accurate prognostic methylation signatures (NDRG2andTLR7), which would be served as a novel therapy target for AML.
Role of Sonic hedgehog signaling in cell cycle, oxidative stress, and autophagy of temozolomide resistant glioblastoma
Authors: Honorato Jessica R. , Hauser‐Davis Rachel A. , Saggioro Enrico M. , Correia Fábio V. , Sales‐Junior Sidney F. , Soares Lorena O. S. , Lima Leandro da R. , Moura‐Neto Vivaldo , Lopes Giselle P. de F. , Spohr Tania C. L. de S. 
Published: October 2019

AbstractThe first‐line chemotherapy treatment for Glioblastoma (GBM) ‐ the most aggressive and frequent brain tumor ‐ is temozolomide (TMZ). The Sonic hedgehog (SHH) pathway is involved with GBM tumorigenesis and TMZ chemoresistance. The role of SHH pathway inhibition in the potentiation of TMZ's effects using T98G, U251, and GBM11 cell lines is investigated herein. The combination of GANT‐61 and TMZ over 72 hr suggested a synergistic effect. All TMZ‐resistant cell lines displayed a significant decrease in cell viability, increased DNA fragmentation and loss of membrane integrity. For T98G cells, G2/M arrest was observed, while U251 cells presented a significant increase in reactive oxygen species production and catalase activity. All the cell lines presented acidic vesicles formation correlated to Beclin‐1 overexpression. The combined treatment also enhanced GLI1 expression, indicating the presence of select resistant cells. The selective inhibition of the SHH pathway potentiated the cytotoxic effect of TMZ, thus becoming a promising in vitro strategy for GBM treatment.
A novel risk signature that combines 10 long noncoding RNAs to predict neuroblastoma prognosis
Authors: Gao Li , Lin Peng , Chen Peng , Gao Rui‐Zhi , Yang Hong , He Yun , Chen Jia‐Bo , Luo Yi‐Ge , Xu Qiong‐Qian , Liang Song‐Wu , Gu Jin‐Han , Huang Zhi‐Guang , Dang Yi‐Wu , Chen Gang 
Published: October 2019

AbstractNeuroblastoma (NBL) is the most frequently encountered extracranial solid neoplasm and impacts significantly on the survival of patients, especially in cases of advanced tumor stage or relapse. A long noncoding RNA (lncRNA) signature to predict the survival of patients with NBL is proposed in this paper. Differentially expressed lncRNA (DElncRNA) was selected using the Limma plus Voom package inRbased on the RNA‐sequencing data downloaded from the Therapeutically Applicable Research To Generate Effective Treatments database and Genotype‐Tissue Expression database. Univariate cox regression analysis, least absolute shrinkage and selection operator regression analysis, and multivariate cox regression analysis were conducted to identify candidate DElncRNAs for the risk signature. Consequently, 10 DElncRNAs were designated as candidate DElncRNAs for the risk signature. Time‐dependent receiver operating characteristic curves and Kapan–Meier survival curves confirmed the efficacy of the risk signature in predicting the survival of patients with NBL (area under the curve = 0.941;p ≤ .001). One of the DElncRNA constituent subparts (LINC01010) was significantly associated with the survival outcome of patients with NBL in GSE62564 (p = .004). Thus, a risk signature comprising 10 DElncRNAs was identified as effective for individual risk stratification and the survival prediction outcomes of patients with NBL.