bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biodegradation of fenpropathrin by Rhodopseudomonas sp. strain PSB07‐21 cultured under three different growth modes
Authors: Luo Xiangwen , Zhang Deyong , Zhou Xuguo , Zhang Songbai , Liu Yong 
Published: March 2019

AbstractThis study aimed at the biodegradation of fenpropathrin by Rhodopseudomonas sp. strain PSB07‐21 cultured under different growth modes. The biomass production, cell surface hydrophobicity and fenpropathrin biodegradation efficiency of the strain PSB07‐21 cultured under the photoheterotrophic growth mode were better than that shown by the strain PSB07‐21 cultured under the photoautotrophic or the chemotrophic growth mode. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis using cell‐free protein extracts showed several distinct protein bands in the gels representing the strain PSB07‐21 cultured under the photoheterotrophic growth mode. The fenpropathrin enzymatic degradation was clearly affected the bacterial growth mode. Results obtained from this study should improve our knowledge regarding fenpropathrin biodegradation under field conditions. Our findings can also be used to optimize the usage of Rhodopseudomonas sp. PSB07‐21 in field applications.
Morphological variability, molecular phylogeny, and biological characteristics of the nematophagous fungus Duddingtonia flagrans
Authors: Wang Bo‐Bo , Zhang Nan , Gong Peng‐Tao , Li Jian‐Hua , Yang Ju , Zhang Hong‐Bo , Zhang Xi‐Chen , Cai Kui‐Zheng 
Published: March 2019

AbstractThis study aims to investigate the molecular phylogenetic analysis, morphological variability, nematode‐capturing ability, and other biological properties of Chinese Duddingtonia flagrans isolates. We isolated 13 isolates of D. flagrans and found features that have never been reported before, such as two to three septa incluing club‐shaped conidia. Meanwhile, we conducted molecular phylogenetic analysis of the seven isolates and tested the radical growth of the isolates under different pH values, temperatures, and media. The capturing ability against infective larvae (L3) of Cooperia spp. in yak was detected in vitro. Finally, one isolate was selected for scanning electron microscopy (SEM) to investigate the trap formation process. The fungal sequence was obtained and submitted to GenBank (Accession no. KY288614.1, KU881774.1, KP257593.1, KY419119.1, MF488979.1, MF488980.1, and MF488981.1), and the tested isolates were identified as D. flagrans. Except for three isolates, the radial growth of the other isolates on 2% corn meal agar and 2% water agar exhibited faster growth than on other media. The fungus could not grow at 10 and 40°C but grew within 11 to 30°C. Moreover, it did not grow at pH 1–3 and 13–14, but instead at pH 4–12. In the in vitro experimental, L3s were reduced by 94.36%, 88.15%, and 91.04% for SDH035, DH055, and F088, respectively. SEM results showed that at 8 hr post addition of nematodes, some of the latter were captured. In the later stages of the interaction of the fungus with nematodes, a large number of chlamydospores were produced, especially on the predation trap. Results of the present study provided information about the molecular phylogenetic analysis, morphological variability, nematode‐capturing ability, and other biological properties of Chinese Arthrobotrys flagrans isolates before administering them for biocontrol.
Mudflat reclamation causes changes of gene abundance in nitrogen cycle under long‐term rice cultivation
Authors: Zhang Yang , Li Qing , Chen Yinglong , Dai Qigen , Hu Jian 
Published: March 2019

AbstractRice cultivation is the main method for mudflat reclamation. However, changes in the community structure of microbes involved in nitrogen (N) cycling in response to mudflat reclamation via rice cultivation remain poorly understood. This study used quantitative polymerase chain reaction to characterize the distribution of various inorganic N‐cycling pathways in response to mudflat reclamation via rice cultivation. The results show that the abundance of functional genes followed an increasing trend, while the relative abundance showed a decreasing trend. The relative richness of functional genes in the inorganic N‐cycling network showed different fluctuation trends and indicated that the nifH, archaea amoA, narG, nirS, nirK, norB, and nosZ genes greatly contribute to inorganic N‐cycling. Redundancy analysis showed that soil properties, in particular, organic matter increased, while electrical conductivity decreased, driving the changes of gene distribution in the inorganic N‐cycling network over the course of reclamation. Mudflat reclamation under long‐term rice cultivation promoted the reproduction of microbes related to the N cycle, and also changed the distribution of functional genes that are involved in the inorganic N cycle due to changes of soil properties.
Responses to copper stress in the metal‐resistant bacterium Cupriavidus gilardii CR3: a whole‐transcriptome analysis
Authors: Huang Ning , Mao Juan , Hu Mingzhong , Wang Xiaoyu , Huo Mingxin 
Published: March 2019

AbstractMicrobial metal‐resistance mechanisms are the basis for the application of microorganisms in metal bioremediation. Despite the available studies of bacterial molecular mechanisms to resistance metals ions (particularly copper), the understanding of bacterial metal resistance is very limited from the transcriptome perspective. Here, responses of the transcriptome (RNA‐Seq) was investigated in Cupriavidus gilardii CR3 exposed to 0.5 mM copper, because strain CR3 had a bioremoval capacity of 38.5% for 0.5 mM copper. More than 24 million clean reads were obtained from six libraries and were aligned against the C. gilardii CR3 genome. A total of 310 genes in strain CR3 were significantly differentially expressed under copper stress. Apart from the routine copper resistance operons cus and cop known in previous studies, Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses of differentially expressed genes indicated that the adenosine triphosphate‐binding cassette transporter, amino acid metabolism, and negative chemotaxis collectively contribute to the copper‐resistant process. More interestingly, we found that the genes associated with the type III secretion system were induced under copper stress. No such results were reordered in bacteria to date. Overall, this comprehensive network of copper responses is useful for further studies of the molecular mechanisms underlying responses to copper stress in bacteria.
Development of Biocompatible and Antibacterial Collagen Hydrogels via Dialdehyde Polysaccharide Modification and Tetracycline Hydrochloride Loading
Authors: Yu Xiaoyue , Yuan Qijuan , Yang Mingtao , Liu Ru , Zhu Shichen , Li Jinling , Zhang Wanni , You Juan , Xiong Shanbai , Hu Yang 
Published: March 2019

AbstractNowadays, collagen hydrogels with both good physicochemical and antibacterial properties for tissue engineering have drawn broad attention. Herein, a biocompatible and antibacterial collagen hydrogel is developed via alginate dialdehyde (ADA) modification and tetracycline hydrochloride (TC) loading based on Schiff's base formation. Fourier transform infrared spectroscopy and X‐ray diffraction spectra suggest the maintenance of collagen structure integrity after ADA modification. The modification significantly contributes to the improved swelling property, resistance against type I collagenase, and strengthens storage modulus of hydrogels with an increase of ADA concentrations. Meanwhile, dynamic release curves of tetracycline hydrochloride (TC)‐loaded hydrogels describe the burst release at the first 15 min then a gradual release, hydrogels act ideally as carriers in antibacterial activity. Furthermore, in vitro biocompatibility and antibacterial properties are successfully confirmed from the fabricated collagen hydrogels. This physicochemical‐ and antibacterial‐property–improved collagen hydrogel would be a potential candidate for wound healing as a scaffold.
Polymorphism of MTHFR 1298A>C in relation to adverse pregnancy outcomes in Chinese populations
Authors: Mo Hui , Rao Meng , Wang Gang , Long Yan‐Xi , Wang Hua‐Wei , Tang Li 
Published: March 2019

AbstractBackgroundAdverse pregnancy outcomes (APOs) are involved in a series of abnormal pregnancies like embryo growth arrest, spontaneous abortion, premature birth, stillbirth, fetal malformation, birth defects and other pathological pregnancy, and childbirth complications. Polymorphism of methylenetetrahydrofolate reductase gene (MTHFR, 607093) is one of the main genetic causes of APO. However, there is still debate on whether MTHFR 1298A>C, rs1801131, polymorphism is related to APO. For the lack of extensive research in the Chinese population at present, the study aim to investigate the relationship between MTHFR 1298A>C polymorphism with APO through a large number of data.MethodsA total of 241 samples from patients with APO and 117 healthy controls in Yunnan province were used for MTHFR gene polymorphism analysis, with double fluorescence quantitative polymerase chain reaction (PCR). In consideration of the low frequency of MTHFR 1298C/C genotype, which might affect the statistic results, further datasets of MTHFR 1298A>C polymorphism were collected from literature and analyzed.ResultsNo statistical difference was detected in the frequency of MTHFR1298A>C polymorphism between two groups in Yunnan. Our data showed that the frequency of MTHFR 1298A/A genotype had the decreasing tendency among Chinese population from northern to southern, as well as eastern to western of China. The frequency of MTHFR 1298A/C and 1298C/C genotypes had the adverse tendency. The frequency of MTHFR 1298C/C genotype was significantly different between two groups in Chinese populations. The significant difference was also observed in the frequency of MTHFR 1298C/C polymorphism between two groups from central China and southern China.ConclusionIn summary, our data showed that MTHFR 1298C/C genotype was one of the important genetic factors of APO in China.
Weight management perceptions and clinical practices among gynaecology providers caring for reproductive‐aged patients
Authors: Evans‐Hoeker E. A. , Ramalingam N. S. , Harden S. M. 
Published: March 2019

SummaryObjectiveResearch suggests that patient and provider conversations about healthy eating and physical activity behaviours may lead to patients' increased health behaviours, access to dietary and physical activity resources, and weight management. The American College of Obstetrics and Gynecology (ACOG) has a number of weight management intervention options, but it is unclear if providers have conversations about intervention options with their patients who are of reproductive age. The purpose of this work was to evaluate the degree to which gynaecology healthcare providers offer the weight management intervention options as recommended by ACOG.MethodsCross‐sectional study of gynaecology providers in Southwest Virginia utilizing an electronic survey to identify weight management perceptions and current clinical practices. Responses were measured using quantitative methods, and agreeability and frequency responses were measured using a 5‐point Likert scale.ResultsTwenty‐three of the 31 eligible providers (74.2%) completed the survey. Providers acknowledge that patients need weight management discussions and they feel comfortable and are willing to have these discussions. While physical activity recommendations were consistent among providers, they did not reflect the complete physical activity recommendations for Americans. Consistency in dietary recommendations was lacking. Although providers make recommendations for physical activity and/or diet at least sometimes, they rarely utilize other methods of weight management as outlined in the ACOG recommendations, such as referrals to other providers, programmes or medications.ConclusionsAreas for improvement in weight management practices include frequency of counselling, consistency in dietary counselling and frequency of utilization of weight loss medications and referrals to ancillary services. These results can be used to aid the development of methods for targeting these deficiencies.
Fire‐safe agent integrated with oyster shell and melamine polyphosphate for thermoplastic polyurethane
Authors: Chen Xilei , Zhang Xinyuan , Wang Wenduo , Wang Yong , Jiao Chuanmei 
Published: March 2019

At present, thermoplastic polyurethane (TPU) is widely used, but there are still many defects in fire safety, such as burning with heavy smoke and dripping. In this article, OS@MP was synthesized by modifying oyster shell (OS) powder with melamine polyphosphate (MP) and then served as fire‐safe agent for TPU. The fire performance of TPU composites were investigated using microscale combustion colorimeter (MCC), cone calorimeter test (CCT), smoke density test (SDT), and thermogravimetric analysis/Fourier transform infrared (TG‐FTIR) spectrum analysis. The MCC and CCT results revealed that OS@MP could reduce the fire hazards of TPU composites. The peak heat release rate (pHRR) of the sample with 10.0 wt% OS@MP decreased to 170.86 kW/m2 from 1772.37 kW/m2 for pure TPU. And, the SDT results showed that OS@MP could significantly reduce the smoke production of TPU composites. The TG‐FTIR also confirmed that the noncombustible gases (including CO2, ammonia, and water vapor) produced by OS@MP have played a reinforcing role in TPU composites as well as a char formed on the surface of composites, which could act as a barrier to prevent the heat and air, reinforce the fire safety of TPU.
Measurable residual disease monitoring using Wilms tumor gene 1 expression in childhood acute myeloid leukemia based on child‐specific reference values
Authors: Løvvik Juul‐Dam Kristian , Guldborg Nyvold Charlotte , Vålerhaugen Helen , Zeller Bernward , Lausen Birgitte , Hasle Henrik , Beier Ommen Hans 
Published: March 2019

AbstractBackgroundMeasurable/minimal residual disease (MRD) monitoring can predict imminent hematological relapse in acute myeloid leukemia (AML). The majority of childhood AML patients do not harbor fusion genes or mutations applicable as MRD markers and overexpression of Wilms tumor gene 1 (WT1) may constitute a useful monitoring target. However, age‐specific reference values in healthy hematopoiesis and standardization of WT1 assessment are prerequisites for clinical utility.ProcedureWe investigated WT1 expression across age in hematologically healthy controls (n = 109), during suspected infection (n = 90) and bone marrow (BM) regeneration (n = 13). WT1 expression in AML at diagnosis (n = 91) and during follow‐up (n = 30) was compared with age‐specific reference values.ResultsWT1 expression correlated with age and showed higher levels in both BM and peripheral blood (PB) in children compared with adults (P < 0.001 and P = 0.01). WT1 expression from healthy hematopoiesis was lower in PB compared with BM (WT1BM/WT1PB = 8.6, 95% CI: 5.3–13.7) and not influenced by infection nor BM regeneration. At AML diagnosis, 66% had more than 20‐fold WT1 overexpression in PB or BM (PB 74%; BM 45%). WT1 was quantified in 279 PB samples during follow‐up. All 11 patients with PB sampling within 4 months of disease recurrence displayed WT1 overexpression by a median of 1.9 months (range, 0.7–9.7) before hematological relapse.ConclusionsThis study defines child‐specific reference values for WT1 expression in healthy hematopoiesis and demonstrates that WT1 expression in PB is a useful post‐treatment monitoring tool in childhood AML. Based on these observations, we propose definitions for childhood AML molecular relapse using WT1 overexpression.
Validation of the school age self‐administered pediatric bleeding questionnaire (Self‐PBQ) in children aged 8–12 years
Authors: Bui Jessica , Martyres David , James Paula D. , Grabell Julie , Wu John , Steele MacGregor , Silva Mariana , Rand Margaret L. , Blanchette Victor S. , Barrowman Nick , Klaassen Robert J. 
Published: March 2019

AbstractBackgroundIn the pediatric population, pathologic bleeding is often challenging to identify. The pediatric bleeding questionnaire (PBQ) was developed as a screening tool for von Willebrand disease (VWD) but was designed to be self‐completed by children above 12 years of age. The study objective was to determine whether a modified Self‐PBQ could be completed by 8‐ to 12‐year‐old children with adult assistance.ProcedureThe initial phase involved seven children who underwent cognitive debriefing to identify problems in the questionnaire, resulting in modifications to wording and response options. In phase 2, children completed the modified Self‐PBQ independently or with assistance from their parent at five Canadian treatment centers. Parents filled out the Self‐PBQ separately to serve as a comparison. Bleeding scores derived from the child self‐report were compared to those of the parent proxy.ResultsTwenty‐nine out of 31 patient/parent pairs successfully completed the Self‐PBQ. Child and parent scores demonstrated a high level of agreement with an intraclass correlation (ICC) of 0.825. In the age subgroup analysis, the ICC was 0.834 and 0.824 for the 8‐ to 9‐year‐old and 10‐ to 12‐year‐old groups, respectively. The ICC was also determined in children with type 1 VWD (ICC = 0.829) versus those with more severe bleeding disorders (ICC = 0.802). Thus, age and disease severity had no significant effect on degree of agreement.ConclusionsOur study shows that agreement was maintained even in younger children aged 8–9 years and in children with varying bleeding phenotypes. This supports the administration of the modified Self‐PBQ to 8‐ to 12‐year‐old children.
Holistic support coupled with prospective tracking reduces abandonment in childhood cancers: A report from India
Authors: Jatia Shalini , Prasad Maya , Paradkar Amey , Bhatia Ameeta , Narula Gaurav , Chinnaswamy Girish , Vora Tushar , Gomle Sanjay , Sankaran Hari , Banavali Shripad 
Published: March 2019

AbstractBackgroundHigh cure rates of over 80% in childhood cancers reported from high‐income countries (HICs) are not replicated in low‐ and middle‐income countries (LMICs). Treatment abandonment (TxA) is an important reason for this poorer outcome. We assessed the effect of a holistic support group approach coupled with prospective tracking on TxA in children with cancer in a limited‐resources environment.MethodsIn 2010, all existing nongovernmental organizations (NGOs) working with childhood cancer at our hospital were brought together to form a pediatric cancer foundation with the aim of providing holistic support to the patient and family, including financial, psychosocial, lodging, educational, and bereavement support. Simultaneously, prospective tracking of all children with a Time‐Responsive Electronic Abandonment Tracking (TREAT) system was also established. The impact of these measures on TxA over the 2009–2016 period was compared using the log‐rank test.ResultsThe annual rate of abandonment reduced from 20% in 2009 to 10.4% in 2010 and 5.2% in 2011. It has been consistently between 3% and 6% from 2012 to 2016 (P −0.04). TxA after the initiation of treatment dropped from 9% in 2009 to 1% in 2016 (P −0.02), while refusal to initiate treatment dropped from 11% to 2.7% (P −0.23) over the same period.ConclusionsA holistic support group consisting of the hospital team, as well as existing NGOs and governmental organizations, along with a systematic and prospective tracking system significantly reduced abandonment in a resource‐constrained setting. This cost‐effective holistic support group may be applicable in other LMICs with similar healthcare systems.
Invasive fusariosis masquerading as extramedullary disease in rapidly progressive acute lymphoblastic leukemia
Authors: Ligon John A. , Natarajan Mukil , Shalabi Haneen , Yates Bonnie , Bishop Rachel , Bianchi David , Alencar Alvaro , Lionakis Michail S. , Shah Nirali N. 
Published: March 2019

AbstractInvasive fusariosis (IF) most commonly occurs in patients with hematologic malignancies and severe neutropenia, particularly during concomitant corticosteroid use. Breakthrough infections can occur in high‐risk patients despite Aspergillus‐active antifungal prophylaxis. We describe a patient with rapid acute lymphoblastic leukemia (ALL) progression who presented with multifocal skin nodules thought to be choloromatous disease. These lesions were ultimately diagnosed as IF and the patient had two simultaneously active disease processes. This case highlights the importance of pathologic diagnosis of new skin lesions in ALL patients, even during leukemia progression, and demonstrates that IF can occur despite normal neutrophil counts and Aspergillus‐active prophylaxis.
The solitary median maxillary central incisor (SMMCI) syndrome: Associations, prenatal diagnosis, and outcomes
Authors: Garcia Rodriguez Raquel , Garcia Cruz Loida , Novoa Medina Yeray , Garcia Delgado Raquel , Perez Gonzalez Julio , Palma Milla Carmen , Lopez Siles Juan , Medina Castellano Margarita , Garcia Hernandez Jose Angel , Santana Rodriguez Alfredo 
Published: March 2019

AbstractSolitary median maxillary central incisor (SMMCI) syndrome is a complex disorder consisting of multiple, developmental defects involving midline structures of the head, which includes the cranial bones, the maxilla, and its container dentition (specifically the central incisor tooth germ), together with other midline structures of the body. SMMCI may appear as an isolated trait or in association with other midline developmental anomalies. We describe the case of a patient with SMMCI. He presented with a solitary median maxillary incisor, short stature, corpus callosum anomalies and a microform of holoprosencephaly (HPE), diabetes insipidus, and neurodevelopmental delay. The diagnosis was performed postnatally based on clinical features, radiological imaging, and a comprehensive genetic study. SMMCI can be diagnosed during the prenatal or neonatal periods or during infancy. Evaluation of the superior maxillary bone is important for prenatal diagnosis. Direct evaluation through bidimensional ultrasound or the use of multiplanar ultrasound or tridimensional reconstruction should be performed in cases of brain or face malformations. Early diagnosis can contribute to improved prenatal assessment and postnatal management.