bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patient-Centered Medical Homes in Community Oncology Practices: Changes in Spending and Care Quality Associated With the COME HOME Experience
Authors: Waters Teresa M. , Kaplan Cameron M. , Graetz Ilana , Price Mary M. , Stevens Laura A. , McAneny Barbara L. 
Published: December 2018

PURPOSE: We examined whether the Community Oncology Medical Home (COME HOME) program, a medical home program implemented in seven community oncology practices, was associated with changes in spending and care quality. PATIENTS AND METHODS: We compared outcomes from elderly fee-for-service Medicare beneficiaries diagnosed between 2011 and 2015 with breast, lung, colorectal, thyroid, or pancreatic cancer, lymphoma, or melanoma and served by COME HOME practices before and after program implementation versus similar beneficiaries served by other geographically proximate oncologists. Difference-in-differences analysis compared changes in outcomes for COME HOME patients versus concurrent controls. Propensity score matching and regression methods were adjusted for clinical and sociodemographic differences. Our primary outcome was 6-month medical spending per beneficiary. Secondary outcomes included 6-month out-of-pocket spending, inpatient and ambulatory care–sensitive hospitalizations, readmissions, length of stay, and emergency department and evaluation and management visits. RESULTS: Before COME HOME, 6-month medical spending was $2,975 higher for the study group compared with controls (95% CI, $1,635 to $4,315; P < .001) and increasing at a similar rate. After intervention, this difference was reduced to $318 (95% CI, −$1,105 to $1,741; P = .661), a significant change of −$2,657 (95% CI, −$4,631 to −$683; P = .008) or 8.1% savings relative to 6-month average spending ($32,866). COME HOME was also associated with significantly reduced (10.2 %) emergency department visits per 1,000 patients per 6-month period ( P = .024). There were no statistically significant differences in other outcomes. CONCLUSION: COME HOME was associated with reduced Medicare spending and improved emergency department use. The patient-centered medical home model holds promise for oncology practices, but improvements were not uniform.
Fibroblast growth factor-6 enhances CDK2 and MAKT expression in microvesicles derived from human stem cells extracted from exfoliated deciduous teeth
Authors: Othman Ahmed , Mubarak Rabab , Sameer Marwa , Sabry Dina 
Published: December 2018

Background: Stem cells from human exfoliated deciduous teeth (SHEDs) are considered one of the most convenient sources of adult stem cells. This study aimed to examine the effect of fibroblast growth factor 6 (FGF-6) on SHEDs and evaluate CDK2 and MATK gene expression in SHED-derived microvesicles (MVs). SHEDs were cultured from deciduous teeth pulp. Methods: SHEDs were divided into two groups: the control group and test groups, with and without FGF-6 supplementation, respectively. After the third passage, SHED proliferation was assessed by MTT assay. MVs were purified and CDK2 and MATK gene expression was assessed by real time polymerase chain reaction. SHEDs were identified by their positivity for CD90 and CD73, and negativity for CD45 and CD34. Results: SHEDs proliferation in the test group was significantly higher than in the control group (P<0.001). mRNA from SHED-derived MVs from the test group exhibited a markedly elevated expression of CDK2 and MAKT, (P<0.002 and P<0.005, respectively) in comparison with those of the control group. FGF-6 enhanced the proliferation of SHEDs. Proliferation enhancement is favorable for the production of a large number of stem cells, which will then be beneficial for cell-based therapies. Conclusions: CDK2 and MAKT genes in SHED-derived MVs can be used as molecular biomarkers for SHED proliferation.
Over the counter: The potential for easing pharmacy provision of family planning in Senegal
Authors: Peterson Jill , Brunie Aurelie , Diop Ibrahima , Diop Seynabou , Stanback John , Chin-Quee Dawn 
Published: December 2018

Background:This research assessed the potential for expanding access to family planning through private sector pharmacies in Senegal, by examining the quality of the services provided through private sector pharmacies, and pharmacy staff and client interest in private sector pharmacy-based family planning services.Methods:This was a cross-sectional, descriptive study conducted in eight urban districts in and around Dakar and two urban districts outside of Dakar employing an audit of 225 pharmacies, a survey with 486 private sector pharmacy staff and a survey with 3,567 women exiting private sector pharmacies.Results:Most (54%) pharmacies reported offering method-specific counseling to clients. Family planning commodities were available in all pharmacies, and 72% had a private space available to offer counseling. Three quarters (76%) did not have any counseling materials available.49% of pharmacists and 47% of assistant pharmacists reported receiving training on family planning during their professional studies. Half had received counseling training. Few pharmacists met pre-determined criteria to be considered highly knowledgeable of the oral contraceptive pill (OCP) and injectable contraceptive provision (0.6% and 1.1%).Overall, 60% of women surveyed were current family planning users and 11% procured their method through a private sector pharmacy. Among non-users of family planning, and current users who did not obtain their method through a pharmacy, 47% said they would be interested in procuring a method through a private sector pharmacy.Conclusions:Senegal’s urban, private sector pharmacies are well-positioned to meet the increasing desire for modern contraception. With proper training, pharmacy staff could better provide effective counseling and provision of OCPs and injectables, and lifting the requirement for a prescription could help support gains in contraceptive prevalence.
The psychophysiological research using the polygraph in the framework of professional selection on the service of the interior ministry in the native language of the candidate
Authors: Скляренко Инна , SKLYARENKO INNA , Синчагова Валентина , Shinagawa Valentina 
Published: December 2018

The article describes the specifics of the special psychophysiological researches with polygraph use, as part of the professional selection of candidates on service in bodies of internal Affairs, insufficient knowledge of the Russian language. The authors discuss the stages of conducting of a special psychophysiological researches with polygraph use by the professional selection of candidates. Each of the stages - preparatory, precipitately actually test, evaluate results, final interview is described taking into account the engagement with the applicant at the service of an interpreter. The article presents the technology of preparation for carrying out of psychophysiological research with two translators, including five blocks, reflects the functional role of the polygraph, the primary and secondary translators, and the sequence of their execution.
The authors draw attention to the fact that the involvement of two translators to the procedure of special psycho-physiological research enables to reveal risk factors of citizens of the Russian Federation, candidates for service in the police Department with insufficient level of knowledge of the Russian language.
Preparing to improve access for young people's mental healthcare
Authors: Crompton Heidi , Hardy Sheila 
Published: December 2018

As half of all mental health problems manifest before the age of 14, access to treatment can seriously affect on long-term health outcomes. Heidi Crompton and Sheila Hardy describe how they prepared general practice staff to better manage young people's mental health Background: It is estimated that half of all mental health problems manifest before the age of 14, with 25% enduring mental health conditions up to the age of 24. However, less than 50% receive treatment at the time. The aim of this project was to prepare staff so that they could improve access to treatment for young people with mental health problems presenting in a general practice setting. Method: Staff at a practice in Dorset, UK, were given training to enable them to recognise when a young person may have a mental health problem and know what actions to take. A computer template was developed to assist them to carry out a safe, detailed assessment of a young person's mental health in a timely manner, and self-help materials were provided. Communication was set up with other organisations, such as Child and Adolescent Mental Health Services, school nursing, and counselling, to address the gap in services that patients experience. Results: Training was received positively. Staff demonstrated increased knowledge and confidence following the session and reported that they would use the template. Use of the template was checked and it had been used eight times in the 4 weeks following the training. A survey carried out 4 months after the training showed that staff thought they were more likely to ask a young person about their mental health and provide them with supportive materials. Conclusion: Staff at the practice felt prepared to manage young people's mental health. There is a need to measure the effect of the interventions on the young people with mental health problems using the service. The plan is to continue and expand the project.
Species Typing of Nontuberculous Mycobacteria by Use of Deoxyribozyme Sensors
Authors: Wood Hillary N , Sidders Ashelyn E , Brumsey Lauren E , Morozkin Evgeny S , Gerasimova Yulia V , Rohde Kyle H 
Published: December 2018

Abstract

BACKGROUND
Nontuberculous mycobacteria (NTM) species are a rising threat, especially to patients living with pulmonary comorbidities. Current point-of-care diagnostics fail to adequately identify and differentiate NTM species from Mycobacterium tuberculosis (Mtb). Definitive culture- and molecular-based testing can take weeks to months and requires sending samples out to specialized diagnostic laboratories.

METHODS
In this proof-of-concept study, we developed an assay based on PCR amplification of 16S ribosomal RNA (rRNA) rrs genes by using universal mycobacterial primers and interrogation of the amplified fragments with a panel of binary deoxyribozyme (BiDz) sensors to enable species-level identification of NTM (BiDz-NTMST). Each BiDz sensor consists of 2 subunits of an RNA-cleaving deoxyribozyme, which form an active deoxyribozyme catalytic core only in the presence of the complimentary target sequence. The target-activated BiDz catalyzes cleavage of a reporter substrate, thus triggering either fluorescent or colorimetric (visually observed) signal depending on the substrate used. The panel included BiDz sensors for differentiation of 6 clinically relevant NTM species (Mycobacterium abscessus, Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium fortuitum, Mycobacterium kansasii, and Mycobacterium gordonae) and Mtb.

RESULTS
Using the fluorescent BiDz-NTMST assay, we successfully identified the species of 38 clinical isolates. In addition, a subset of strains was tested with visual BiDz sensors, providing proof-of-concept for species typing of NTM by the naked eye.

CONCLUSIONS
The BiDz-NTMST assay is a novel platform for rapid identification of NTM species. This method is highly specific and significantly faster than current tools and is easily adaptable for onsite diagnostic laboratories in hospitals or clinical laboratories.
Concurrent Single-Cell RNA and Targeted DNA Sequencing on an Automated Platform for Comeasurement of Genomic and Transcriptomic Signatures
Authors: Kong Say Li , Li Huipeng , Tai Joyce A , Courtois Elise T , Poh Huay Mei , Lau Dawn Pingxi , Haw Yu Xuan , Iyer Narayanan Gopalakrishna , Tan Daniel Shao Weng , Prabhakar Shyam , Ruff Dave , Hillmer Axel M 
Published: December 2018

Abstract

BACKGROUND
The comeasurement of both genomic and transcriptomic signatures in single cells is of fundamental importance to accurately assess how the genetic information correlates with the transcriptomic phenotype. However, existing technologies have low throughput and laborious work flows.

METHODS
We developed a new method for concurrent sequencing of the transcriptome and targeted genomic regions (CORTAD-seq) within the same single cell on an automated microfluidic platform. The method was compatible with the downstream library preparation, allowing easy integration into existing next-generation sequencing work flows. We incorporated a single-cell bioinformatics pipeline for transcriptome and mutation analysis.

RESULTS
As proof of principle, we applied CORTAD-seq to lung cancer cell lines to dissect the cellular consequences of mutations that result in resistance to targeted therapy. We obtained a mean detection of 6000 expressed genes and an exonic rate of 50%. The targeted DNA-sequencing data achieved a 97.8% detection rate for mutations and allowed for the identification of copy number variations and haplotype construction. We detected expression signatures of tyrosine kinase inhibitor (TKI) resistance, epidermal growth factor receptor (EGFR) amplification, and expansion of the T790M mutation among resistant cells. We also identified characteristics for TKI resistance that were independent of EGFR T790M, indicating that other alterations are required for resistance in this context.

CONCLUSIONS
CORTAD-seq allows assessment of the interconnection between genetic and transcriptomic changes in single cells. It is operated on an automated, commercially available single-cell isolation platform, making its implementation straightforward.
PERAN SERTA MASYARAKAT DAN KEWENANGAN PEMERINTAH DALAM KONSERVASI MANGROVE SEBAGAI UPAYA MENCEGAH ROB DAN BANJIR SERTA SEBAGAI TEMPAT WISATA
Authors: Turisno Bambang Eko , Suharto R. , Priyono Ery Agus 
Published: December 2018

Penelitian bertujuan menganalisis peran serta masyarakat dan kewenangan Pemerintah dalam konservasi mangrove sebagai upaya mencegah rob dan banjir serta sebagai Tempat Wisata. Metode pendekatan yang digunakan adalah normatif, spesifikasi penelitian deskriptif analitis, analisis menggunakan metode analisis kualitatif. Data-data yang dipergunakan adalah data sekunder, meliputi informasi tentang peran serta masyarakat, kewenangan Pemerintah, wisata mangrove, konservasi hutan serta bahan-bahan hukum maupun data penunjang lainnya. Hutan mangrove dimanfaatkan sebagai budidaya, sebagai upaya mencegah banjir dan rob, hutan mangrove juga sebagai tempat konservasi dan tempat wisata. Penanaman pohon mangrove sebagai rehabilitasi pantai dilakukan dengan langsung ditanam di tanah atau dengan teknik bronjong serta melibatkan masyarakat. Partisipasi masyarakat dalam pengelolaan ekosistem mangrove disamping agar terjaga konservasi mangrove juga akan meningkatan kesejahteraan masyarakat. Kolaborasi antara sektor wisata, pemerintah daerah dan masyarakat dalam konservasi mangrove harus diintegrasikan. Kontribusi Penelitian sebagai masukan bagi pemerintah khususnya pemerintah daerah dan masyarakat dalam memanfaatkan hutan mangrove dengan menjaga konservasinya.
Cell proliferation induced by modified cationic dextran
Authors: Kamiński Kamil , Stalińska Krystyna , Niziołek Anna , Wróbel Maria , Nowakowska Maria , Kaczor-Kamińska Marta 
Published: December 2018

Abstract
The interaction between oppositely charged membranes and polycations causes cell aggregation, loss of membrane fluidity, and membrane degeneration and may cause an increase of its permeability. Unfortunately, the interaction is the reason why the use of polycations in medicine is severely limited. Therefore, in this paper, we share our observations related to the action of 40-kDa dextran modified using glycidyltrimethylammonium chloride, resulting in increased fibroblast cell proliferation. Using viability and proliferation tests [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, crystal violet, 3H-thymidine incorporation], we have observed that cationic dextran derivatives exert a positive impact on nonepithelial cell proliferation in vitro. This phenomenon has been noted for human and mouse fibroblasts and several other nonepithelial cell lines. However, the effect seems to be most pronounced for fibroblast cell lines. The presented studies allow to examine the impact of the polymer structure and the methods of its cationic modification on this newly observed phenomenon. The observation is unique because positively charged macromolecules usually exhibit high toxicity in all cell types in vitro.
Thirty-minutes’ exposure to smartphone call triggers neutrophil activation in vitro
Authors: Lippi Giuseppe , Danese Elisa , Brocco Giorgio , Benati Marco , Salvagno Gian Luca , Montagnana Martina , Franchini Massimo 
Published: December 2018

Abstract

Background:
Despite accumulating evidence about the negative health effects of exposure to electromagnetic fields emitted by mobile phones, no information is available on the potential impact of radiofrequency (RF) waves on polymorphonuclear leukocytes biology.

Methods:
Two sequential whole blood tubes were collected from 16 ostensibly healthy volunteers. After placing the former tube of each subject in a plastic rack, 1 cm from a commercial smartphone (carrier frequency, 900 MHz), a call was placed on the smartphone and a communication lasting 30 min was manually activated. The latter blood tube of each volunteer was placed in another plastic rack, for an identical period of time, avoiding close contact with sources of RF waves. A complete blood count was then assessed in all whole blood samples, using Advia 2120.

Results:
The 30-min exposure of blood to RF waves did not induce significant variations of total and differential leukocyte counts. A significant decrease was however observed for many neutrophils parameters, with median percentage variation of −3.9% for the lobularity index (LI), −29.8% for the myeloperoxidase index (MPXI), −0.6% for the neutrophil cluster mean x (NEUTx) and −0.7% for the neutrophil cluster mean y (NEUTy), respectively. The percentage of blood samples with reduced values after exposure to RF waves was 81% for LI, 88% for NEUTx and 100% for both MPXI and NEUTy.

Conclusions:
The results of this study show that exposure to smartphone RF waves triggers activation of neutrophils in vitro, as mirrored by the significant variations observed in many activation parameters in Advia 2120.
Prenatal detection and obstetric management of true umbilical cord knots using color Doppler and 4D ultrasonography: a report of two cases and literature review
Authors: Gokmen Karasu Ayse Filiz , Kutuk Mehmet Serdar 
Published: December 2018

Abstract

Background
True umbilical cord knots are common incidental findings at birth, however, there are limited reports of antenatal diagnosis. True knots are mostly asymptomatic; however, cord related accidents may be encountered.

Case presentation
We present two cases we diagnosed and followed-up antenatally. We reviewed previous literature and found 16 reports of antenatal diagnosis. In addition to foregoing established risk factors, we noticed an increased risk at first trimester screening in our cases.

Conclusions
Modern ultrasonography (USG) technology applications are valuable for monitoring knot architecture and compression. We propose monitoring the baby with daily kick counts and biweekly cardiotocography after 28–30 weeks of gestation. Weekly umbilical Doppler pulsatility (PI) measurements of afferent and efferent loops with respect to the gestational age, aids in monitoring the tightness of the knot. The timing and route of delivery should be necessitated by obstetric indications. Vaginal delivery is possible under continuous fetal monitorization during labor.
Three laparotomies later: extrinsic duodenal atresia from malrotation complicated by intrinsic duodenal and pyloric windsock webs
Authors: Bengtsson Bengt-Ola S. , Lee Sang , Houten John P. van , Stolar Charles J.H. 
Published: December 2018

Abstract
We present a term infant with the unusual findings of malrotation in combination with both duodenal and pyloric webs of windsock type, thus masquerading as postoperative bowel dysfunction and ultimately requiring three laparotomies. To the best of our knowledge these three findings in combination have not been previously reported. The dilemma of whether to perform a duodenotomy or not during malrotation repair and the importance of instrumenting the intestine from the duodenotomy site in both retrograde and antegrade directions are discussed.
Warum Männlichkeit ein Thema der Degrowth-Bewegung sein sollte
Authors: Heilmann Andreas , Scholz Sylka 
Published: December 2018

AbstractAusgangspunkt ist die Beobachtung, dass in den Debatten der Degrowth-Bewegung das Thema Männlichkeit bisher kaum eine Rolle spielt. Dies ist insofern überraschend, als der Aufstieg des wachstumsorientierten Kapitalismus mit der Herausbildung einer spezifischen männlichen Subjektivierungsweise verknüpft ist. Anhand des Konzeptes der imperialen Lebensweise (Brand/Wissen) diskutieren wir exemplarisch, wie die Kategorie Männlichkeit in die Analyse kapitalistischer Wachstumsgesellschaften einbezogen werden kann und welche Erkenntnisse dadurch zu gewinnen sind. Anschließend wenden wir uns der Frage nach möglichen Bündnissen zwischen gleichstellungsorientierten Männlichkeitspolitiken und Degrowth-Bewegung zu. Wir argumentieren, dass sie sich bezüglich einer Politik der De-Privilegierung überschneiden und sich gegenseitig stärken könnten, um eine solidarische Lebensweise auf den Weg zu bringen.
Directed evolution of the 3C protease from coxsackievirus using a novel fluorescence-assisted intracellular method
Authors: Meister Sebastian W. , Hendrikse Natalie M. , Löfblom John 
Published: December 2018

Abstract
Proteases are crucial for regulating biological processes in organisms through hydrolysis of peptide bonds. Recombinant proteases have moreover become important tools in biotechnological, and biomedical research and as therapeutics. We have developed a label-free high-throughput method for quantitative assessment of proteolytic activity in Escherichia coli. The screening method is based on co-expression of a protease of interest and a reporter complex. This reporter consists of an aggregation-prone peptide fused to a fluorescent protein via a linker that contains the corresponding substrate sequence. Cleavage of the substrate rescues the fluorescent protein from aggregation, resulting in increased fluorescence that correlates to proteolytic activity, which can be monitored using flow cytometry. In one round of flow-cytometric cell sorting, we isolated an efficiently cleaved tobacco etch virus (TEV) substrate from a 1:100 000 background of non-cleavable sequences, with around 6000-fold enrichment. We then engineered the 3C protease from coxsackievirus B3 (CVB3 3Cpro) towards improved proteolytic activity on the substrate LEVLFQ↓GP. We isolated highly proteolytic active variants from a randomly mutated CVB3 3Cpro library with up to 4-fold increase in activity. The method enables simultaneous measurement of proteolytic activity and protease expression levels and can therefore be applied for protease substrate profiling, as well as directed evolution of proteases.