bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brian Wilson Reimagined: The Reparative Portrait in Love & Mercy
Authors: MCQUISTON KATE 
Published: August 2019

AbstractThe 50-year anniversary of the Beach Boys’ seminal album Pet Sounds and Brian Wilson's corresponding world concert tour have once again brought attention to Wilson and his creative work with the Beach Boys. These events have brought about new recording releases and publications about the band and Wilson, including the first feature-length biopic on Wilson, Love &amp; Mercy. The following essay investigates Atticus Ross's reimaginative approach to Brian Wilson's music for the soundtrack of this film, directed by Bill Pohlad and released in 2014. The film expands recent trends in music biopics of the last couple of decades regarding the mobilization and activation of music to afford new interpretations of their subjects and new ways to hear their work. Ross's approach is distinct for its extensive recomposition of Brian Wilson's music in the film's original score, which allows director Bill Pohlad to show Wilson in a new light.Ross incorporates Beach Boys recordings (studio sessions and released tracks) into new pieces that highlight processes of manipulation, layering, and repetition, which point to the studio as a major site of Wilson's creativity. These processes furthermore portray the psychic life of the film's characters. Ross's compositions dramatize the subjectivity of hearing and rogue behaviors of auditory recollection and hallucination that characterize both Wilson's creativity and mental illness. In the context of the story of Brian Wilson and Melinda Ledbetter, Ross's compositions take on palliative associations that have direct implications for the reception of the film's original soundtrack.
Wildfire Impact on Environmental Thermodynamics and Severe Convective Storms
Authors: Zhang Yuwei , Fan Jiwen , Logan Timothy , Li Zhanqing , Homeyer Cameron R. 
Published: August 2019

AbstractWildfires are extreme events associated with weather, climate, and environment and have been increasing globally in frequency, burn season length, and burned area. It is of great interest to understand the impacts of wildfires on severe convective storms through releasing heat and aerosols into the atmosphere. We have developed a model capability that can account for the impact of sensible heat fluxes from wildfires on thermodynamics and is computationally efficient. The pyrocumulonimbus clouds associated with the Texas Mallard Fire on 11–12 May 2018 are well simulated by accounting for both heat and aerosols emitted from the wildfire. Both heat and aerosol effects increase low‐level temperatures and midlevel buoyancy and enhance convective intensity. Intensified convection along with more supercooled liquid condensate due to stronger vertical transport results in larger hailstones and enhanced lightning. The effects of heat flux on the convective extremes are more significant than those of aerosol emissions.
Long‐Term Trends in the Low‐Latitude Middle Atmosphere Temperature and Winds: Observations and WACCM‐X Model Simulations
Authors: Venkat Ratnam M. , Akhil Raj S.T. , Qian Liying 
Published: August 2019

AbstractIn recent years, the middle atmosphere has evoked great scientific interest as long‐term changes can be clearly captured owing to the large perturbation amplitudes at these altitudes. In the present study, more than 25 years of the data are used to investigate the long‐term trends in the middle atmosphere by suitably combining the observations from different techniques (Rocketsonde, High‐Resolution Doppler Imager (HRDI)/Upper Atmosphere Research Satellite (UARS), Halogen Occultation Experiment (HALOE)/UARS, Sounding of the Atmosphere using Broadband Emission Radiometry (SABER)/Thermosphere Ionosphere Mesosphere Energetic Dynamics (TIMED), and Mesosphere‐stratosphere‐troposphere (MST) radar) from Indian region. As different instruments/techniques are used and the time periods are not the same, extreme care has been taken while merging various data sets to obtain meaningful long‐term trends. To understand the observed long‐term trends, Whole Atmosphere Community Climate Model–eXtended model simulations for the Indian low‐latitude regions are also used. A significant cooling trend of ~−1.7 ± 0.5 K/decade between 30 and 80 km is noticed. Large decreasing trend (~5 m/s/decade) in the eastward winds is noticed which is significant between 70 and 80 km only changing from strong eastward wind in 1970s to weak westward wind in recent decade. No significant trends are observed in the meridional wind. These observations are well captured by the Whole Atmosphere Community Climate Model–eXtended model simulations while considering changes in concentrations of greenhouse gases including carbon dioxide (CO2), methane (CH4), water vapor (H2O), and chlorofluorocarbon species that cause depletion of stratospheric ozone (O3). Thus, it is prudent to conclude that long‐term decreasing trends in the zonal winds and cooling trends in the temperature in the middle atmosphere are caused to a large extent by greenhouse gases suggesting the role of anthropogenic changes in the dynamics of the middle atmosphere.
Branched hybridization chain reaction—using highly dimensional DNA nanostructures for label-free, reagent-less, multiplexed molecular diagnostics
Authors: Xu Gaolian , Lai Mingliang , Wilson Rab , Glidle Andrew , Reboud Julien , Cooper Jonathan M. 
Published: August 2019

AbstractThe specific and multiplexed detection of DNA underpins many analytical methods, including the detection of microorganisms that are important in the medical, veterinary, and environmental sciences. To achieve such measurements generally requires enzyme-mediated amplification of the low concentrations of the target nucleic acid sequences present, together with the precise control of temperature, as well as the use of enzyme-compatible reagents. This inevitably leads to compromises between analytical performance and the complexity of the assay. The hybridization chain reaction (HCR) provides an attractive alternative, as a route to enzyme-free DNA amplification. To date, the linear nucleic acid products, produced during amplification, have not enabled the development of efficient multiplexing strategies, nor the use of label-free analysis. Here, we show that by designing new DNA nanoconstructs, we are able, for the first time, to increase the molecular dimensionality of HCR products, creating highly branched amplification products, which can be readily detected on label-free sensors. To show that this new, branching HCR system offers a route for enzyme-free, label-free DNA detection, we demonstrate the multiplexed detection of a target sequence (as the initiator) in whole blood. In the future, this technology will enable rapid point-of-care multiplexed clinical analysis or in-the-field environmental monitoring.
miR-124/VAMP3 is a novel therapeutic target for mitigation of surgical trauma-induced microglial activation
Authors: Chen Yan , Sun Jing-xian , Chen Wan-kun , Wu Gen-cheng , Wang Yan-qing , Zhu Ke-ying , Wang Jun 
Published: August 2019

AbstractActivation of microglia and the subsequently elevated inflammatory cytokine release in the brain during surgery predispose individuals to cognitive dysfunction, also known as postoperative cognitive dysfunction (POCD). miR-124 is one of the most abundant microRNAs in the brain that regulates microglial function. Elucidating the role of miR-124 in microglial activation in the context of surgery may therefore promote understanding of as well as therapeutic development for post-surgical disorders involving microglial activation. The downstream targets of miR-124 were investigated using bioinformatic screening and dual-luciferase reporter assay validation, and vesicle-associated membrane protein 3 (VAMP3) was identified as a potential target. The kinetics of miR-124/VAMP3 expression was first examined in vitro in microglial cells (primary microglia and BV2 microglial cells) following lipopolysaccharide (LPS) stimulation. LPS induced a time-dependent decrease of miR-124 and upregulated the expression of VAMP3. Manipulating miR-124/VAMP3 expression by using miR-124 mimics or VAMP3-specific siRNA in LPS-stimulated BV2 microglial cells inhibited BV2 microglial activation-associated inflammatory cytokine release. To further examine the role of miR-124/VAMP3 in a surgical setting, we employed a rat surgical trauma model. Significant microglial activation and altered miR-124/VAMP3 expression were observed following surgical trauma. We also altered miR-124/VAMP3 expression in the rat surgical trauma model by administration of exogenous miR-124 and by using electroacupuncture, which is a clinically applicable treatment that modulates microglial function and minimizes postoperative disorders. We determined that electroacupuncture treatment specifically increases the expression of miR-124 in the hypothalamus and hippocampus. Increased miR-124 expression with a concomitant decrease in VAMP3 expression resulted in decreased inflammatory cytokine release related to microglial activation post-surgery. Our study indicates that miR-124/VAMP3 is involved in surgery-induced microglial activation and that targeting miR-124/VAMP3 could be a potential therapeutic strategy for postoperative disorders involving microglial activation.
A descriptive follow-up interview study assessing patient-centred outcomes: Salford Lung Study in Asthma (SLS Asthma)
Authors: Doward Lynda , Svedsater Henrik , Whalley Diane , Crawford Rebecca , Leather David , Lay-Flurrie James , Bosanquet Nick 
Published: August 2019

AbstractThe Salford Lung Study in Asthma (SLS Asthma) was a multicentre, randomised, controlled, open-label trial that assessed initiating once-daily, single-inhaler fluticasone furoate/vilanterol (FF/VI) 100 μg/25 μg or 200 μg/25 μg versus continuing usual care. A subgroup (n = 400) from SLS Asthma was enrolled in this exploratory, interview-based follow-up study. Quantitative and qualitative data were collected via questionnaires. The primary objective was to capture patient-centred outcomes (symptom experience, quality of life [QoL], disease management behaviours) and patient experience. Secondary objectives were to assess the correlation of patient-reported outcomes with pre-defined variables from SLS Asthma (Asthma Control Test [ACT] score). The follow-up sample was representative of the SLS Asthma population; half reported asthma improvement during the study. Breathlessness was the most likely symptom to improve (47.8% of patients reported improvement). Most patients reported ‘no change’ in overall QoL (57.5%) and daily life domains (functioning 66.3%, activities 68.3%, relationships 86.8%, psychological 68.5%). Functioning was reported as the most frequently improved domain (29.8% of patients). Perceived improvement in asthma control (42.5%) and confidence (37.3%) was frequent. ACT responders (defined as patients achieving an ACT score ≥20 and/or an increase of ≥3 in ACT score from baseline at Week 52) were more likely to report asthma improvement (88.7% of patients reporting ‘a lot’ of improvement) than non-responders. Patients’ asthma experiences generally improved during SLS Asthma. Clinical improvements were often associated with perceived improvement by patients, particularly among ACT responders.
Numerical Modeling of the Dynamics of Malaria Transmission in a Highly Endemic Region of India
Authors: Singh Parihar Ruchi , Bal Prasanta Kumar , Kumar Vaibhav , Mishra Saroj Kanta , Sahany Sandeep , Salunke Popat , Dash Sushil Kumar , Dhiman Ramesh Chand 
Published: August 2019

AbstractUsing a dynamical model (VECTRI) for malaria transmission that accounts for the influence of population and climatic conditions, malaria transmission dynamics is investigated for a highly endemic region (state of Odisha) in India. The model is first calibrated over the region, and subsequently numerical simulations are carried out for the period 2000–2013. Using both model and observations we find that temperature, adult mosquito population, and infective biting rates have increased over this period, and the malaria vector abundance is higher during the summer monsoon season. Regionally, the intensity of malaria transmission is found to be higher in the north, central and southern districts of Odisha where the mosquito populations and the number of infective bites are more and mainly in the forest or mountainous ecotypes. We also find that the peak of the malaria transmission occurs when the monthly mean temperature is in the range of ~28–29 °C, and monthly rainfall accumulation in the range of ~200–360 mm.
Influence of AgVO3 incorporation on antimicrobial properties, hardness, roughness and adhesion of a soft denture liner
Authors: Kreve Simone , Oliveira Viviane C. , Bachmann Luciano , Alves Oswaldo L. , Reis Andréa C. Dos 
Published: August 2019

AbstractThe objective of this in vitro study was to investigate the effect of nanostructured silver vanadate decorated with silver nanoparticles (AgVO3) on antimicrobial activity, hardness, roughness, and adhesion of a soft denture liner. The antimicrobial efficacy of the Trusoft (Boswoth) liner incorporated with different concentrations of AgVO3 against Enterococcus faecalis, Pseudomonas aeruginosa, Candida albicans, and Staphyloccocus aureus (n = 5) was evaluated by the agar diffusion method. Roughness, hardness, and adhesion properties were also evaluated. The data were analyzed by analysis of variance (ANOVA) and Tukey’s multiple comparison test with significance at the p < 0.05 level. At concentrations of 1 and 2.5%, AgVO3 incorporation was effective only against E. faecalis, and at 5 and 10%, against E. faecalis, P. aeruginosa, and C. albicans. None of the concentrations was effective against S. aureus. A decrease in hardness was found for the 1, 2.5, and 10% AgVO3 concentrations (p < 0.001) and at 5%, hardness was not affected. None of the concentrations affected the roughness of the material. A significant increase in tensile values was observed between the liner and heat-curing acrylic resin for 2.5% (p < 0.001) and 10% (p = 0.042) concentrations. AgVO3 incorporation to a soft denture liner promoted antimicrobial activity against E. faecalis, P. aeruginosa, and C. albicans without affecting roughness, maintaining the hardness properties recommended for soft and extra soft liners, and improving the adhesion between the liner and the acrylic resin used for dentures.
Peptide translocation by the lysosomal ABC transporter TAPL is regulated by coupling efficiency and activation energy
Authors: Bock Christoph , Zollmann Tina , Lindt Katharina-Astrid , Tampé Robert , Abele Rupert 
Published: August 2019

AbstractThe lysosomal polypeptide transporter TAPL belongs to the superfamily of ATP-binding cassette transporters. TAPL forms a homodimeric transport complex, which translocates oligo- and polypeptides into the lumen of lysosomes driven by ATP hydrolysis. Although the structure and the function of ABC transporters were intensively studied in the past, details about the single steps of the transport cycle are still elusive. Therefore, we analyzed the coupling of peptide binding, transport and ATP hydrolysis for different substrate sizes. Although longer and shorter peptides bind with the same affinity and are transported with identical Km values, they differ significantly in their transport rates. This difference can be attributed to a higher activation energy for the longer peptide. TAPL shows a basal ATPase activity, which is inhibited in the presence of longer peptides. Uncoupling between ATP hydrolysis and peptide transport increases with peptide length. Remarkably, also the type of nucleotide determines the uncoupling. While GTP is hydrolyzed as good as ATP, peptide transport is significantly reduced. In conclusion, TAPL does not differentiate between transport substrates in the binding process but during the following steps in the transport cycle, whereas, on the other hand, not only the coupling efficiency but also the activation energy varies depending on the size of peptide substrate.
Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken
Authors: Oh Euna , Andrews Katelyn J. , McMullen Lynn M. , Jeon Byeonghwa 
Published: August 2019

AbstractCampylobacter jejuni is a microaerophilic foodborne pathogen that is sensitive to stress conditions. However, it is not yet understood how this stress-sensitive pathogen may cause a significant number of cases of human gastroenteritis worldwide. In this study, we examined stress tolerance in 70 C. jejuni strains isolated from retail chicken under several stress conditions related to food safety. Compared to oxygen-sensitive (OS) strains of C. jejuni, C. jejuni strains with increased aerotolerance, such as hyper-aerotolerant (HAT) and aerotolerant (AT) strains, were more tolerant to peracetic acid, refrigeration and freeze-thaw stresses. However, the levels of thermotolerance and hyper-osmotolerance were not associated with the aerotolerance level of C. jejuni. The HAT and AT strains of C. jejuni exhibited significantly increased activities of catalase and superoxide dismutase (SOD), compared to the OS strains. Consistently, the HAT and AT strains were highly tolerant to oxidants, such as hydrogen peroxide, cumene hydroperoxide and menadione, compared to the OS strains. The AT and HAT strains that were tolerant to stresses, particularly peracetic acid and refrigeration, predominantly belonged to multilocus sequence typing (MLST) clonal complex (CC)-21. This study shows that oxidative stress resistance plays a role in determining the differential level of aerotolerance in C. jejuni and that AT and HAT strains of C. jejuni are more tolerant to oxidants and low temperatures than OS strains.
Developing well-calibrated illness severity scores for decision support in the critically ill
Authors: Cosgriff Christopher V. , Celi Leo Anthony , Ko Stephanie , Sundaresan Tejas , Armengol de la Hoz Miguel Ángel , Kaufman Aaron Russell , Stone David J. , Badawi Omar , Deliberato Rodrigo Octavio 
Published: August 2019

AbstractIllness severity scores are regularly employed for quality improvement and benchmarking in the intensive care unit, but poor generalization performance, particularly with respect to probability calibration, has limited their use for decision support. These models tend to perform worse in patients at a high risk for mortality. We hypothesized that a sequential modeling approach wherein an initial regression model assigns risk and all patients deemed high risk then have their risk quantified by a second, high-risk-specific, regression model would result in a model with superior calibration across the risk spectrum. We compared this approach to a logistic regression model and a sophisticated machine learning approach, the gradient boosting machine. The sequential approach did not have an effect on the receiver operating characteristic curve or the precision-recall curve but resulted in improved reliability curves. The gradient boosting machine achieved a small improvement in discrimination performance and was similarly calibrated to the sequential models.
The synthesis of branched-chain fatty acids is limited by enzymatic decarboxylation of ethyl- and methylmalonyl-CoA
Authors: Dewulf Joseph P. , Gerin Isabelle , Rider Mark H. , Veiga-da-Cunha Maria , Van Schaftingen Emile , Bommer Guido T. 
Published: August 2019

Abstract
Most fatty acids (FAs) are straight chains and are synthesized by fatty acid synthase (FASN) using acetyl-CoA and malonyl-CoA units. Yet, FASN is known to be promiscuous as it may use methylmalonyl-CoA instead of malonyl-CoA and thereby introduce methyl-branches. We have recently found that the cytosolic enzyme ECHDC1 degrades ethylmalonyl-CoA and methylmalonyl-CoA, which presumably result from promiscuous reactions catalyzed by acetyl-CoA carboxylase on butyryl- and propionyl-CoA. Here, we tested the hypothesis that ECHDC1 is a metabolite repair enzyme that serves to prevent the formation of methyl- or ethyl-branched FAs by FASN. Using the purified enzyme, we found that FASN can incorporate not only methylmalonyl-CoA but also ethylmalonyl-CoA, producing methyl- or ethyl-branched FAs. Using a combination of gas-chromatography and liquid chromatography coupled to mass spectrometry, we observed that inactivation of ECHDC1 in adipocytes led to an increase in several methyl-branched FAs (present in different lipid classes), while its overexpression reduced them below wild-type levels. In contrast, the formation of ethyl-branched FAs was observed almost exclusively in ECHDC1 knockout cells, indicating that ECHDC1 and the low activity of FASN toward ethylmalonyl-CoA efficiently prevent their formation. We conclude that ECHDC1 performs a typical metabolite repair function by destroying methyl- and ethylmalonyl-CoA. This reduces the formation of methyl-branched FAs and prevents the formation of ethyl-branched FAs by FASN. The identification of ECHDC1 as a key modulator of the abundance of methyl-branched FAs opens the way to investigate their function.
Analytische Charakterisierung und Vergleich medizinisch genutzter Echinacea-haltiger Zubereitungen
Authors: Classen Birgit , Pferschy-Wenzig Eva-Maria , Geske Thomas , Ardjomand-Wölkart Karin , Bauer Rudolf 
Published: August 2019

ZusammenfassungDie drei Sonnenhutarten Echinacea angustifolia, E. pallida und E. purpurea werden als Ausgangsmaterial für die Produktion phytotherapeutischer und homöopathischer Zubereitungen genutzt, welche als unspezifische Immunstimulanzien Einsatz finden. Dabei sind vermutlich sowohl nieder- als auch hochmolekulare Inhaltsstoffe an der Wirkung beteiligt. Aus den verschiedenen Pflanzen und Pflanzenteilen entstehen durch Anwendung unterschiedlicher Herstellungsmethoden aufgrund der Vorgaben der Arzneibücher Zubereitungen, die erhebliche Unterschiede in ihrer stofflichen Zusammensetzung erwarten lassen. In den vorliegenden Untersuchungen wurden daher verschiedene Echinacea-haltige Zubereitungen hinsichtlich des Vorkommens von Kaffeesäurederivaten, Alkamiden, Polysacchariden und Proteoglykanen analytisch charakterisiert und verglichen.Erwartungsgemäß zeigte sich, dass sich homöopathische Urtinkturen bzw. alkoholische Extrakte sowohl hinsichtlich der hochmolekularen Inhaltsstoffe (Polysaccharide, Proteoglykane) als auch bei den polaren und lipophilen niedermolekularen Inhaltsstoffen von Echinacea-purpurea-Presssaftzubereitungen deutlich unterscheiden und sie daher nicht als äquivalent angesehen werden können. Auch zwischen den homöopathischen Urtinkturen der einzelnen Arten existieren Unterschiede und selbst innerhalb der Echinacea-purpurea-Presssaftzubereitungen konnten, je nach Herstellungsart, verschiedene Inhaltsstoffmuster detektiert werden. Bei der Bewertung von Wirksamkeit und Unbedenklichkeit von Echinacea-Zubereitungen sollten diese Unterschiede in Zukunft mehr berücksichtigt werden.
3D Printed Model Used as Preoperative Tool for Treating Acromioclavicular Joint Dislocation with Pre-Contoured Clavicle Hook Plate: Technical Note
Authors: Wu Xuhua , Wang Gang , Rong Ke , Xia Qingquan , Gan Minfeng , Wen Gen , Yin Xiaofan , Yang Huilin 
Published: August 2019

Abstract
Objective To introduce a new technique using real-size 3D-printed acromioclavicular joint models as preoperative tools for treating acromioclavicular (AC) joint dislocation.
Methods Both the injured AC joint and the mirrored normal side AC joint are 3D printed into real-size models. A proper hook plate is selected and pre-bended to fit the normal side AC joint. The bended plate is then used to perform a reduction operation on a real-size injured AC joint model and the result is compared with the mirrored normal AC joint model to see if the reduction is accurate.
Results It is easy to select and bend a clavicle hook plate to achieve an accurate reduction with the help of a 3D printing technique.
Conclusion With this technique, surgeons can achieve an accurate reduction of the injured AC joint with a clavicle hook plate which might be helpful to reduce the risk of complications.