bluuebunny/crossref_metadata_2025_split huggingface dataset:
Concept of chemical reaction in chemistry textbooks
Authors: Michalisková Renata , Haláková Zuzana , Prokša Miroslav 
Published: October 2019

Abstract
For more than 20 years the science teachers, the researchers in science education, the educationists and the other pedagogues have been acquainted with the model of educational reconstruction. In this model there are integrated three areas of this scientific interest: the recognition of pupils’ conceptions, the investigation of scientific conceptions with didactics branch approach and the creation of an educational content (Duit, R., Gropengießer, H., & Kattmann, U. (2005). Towards science education research that is relevant for improving practice: The model of educational reconstruction. In H. E. Fischer, (Ed.), The Developing standards in research on science education (pp. 1–9). London: Taylor & Francis; Duit, R., Gropengießer, H., Kattmann, U., Komorek, M., & Parchmann, I. (2012). The model of educational reconstruction – a framework for improving teaching and learning science. In D. Jorde & J. Dillon (Eds.), Science education research and practice in Europe. Cultural perpectives in science education, 5 (pp. 13–37). Rotterdam: Sense Publishers; Kattmann, U., Duit, R., Gropengießer, H., & Komorek, M. (1997). Das Modell der Didaktischen Rekonstruktion – Ein Rahmen für naturwissenschaftsdidaktische Forschung und Entwicklung.Zeitschrift für Didaktik der Naturwissenschaften, 3(3), 3–18). There is required to reconstruct the educational content for learning and teaching purposes. It leads to the educational structuring and to the clarification in scientific field. Therefore, in this contribution there is analysed a formation of the concept chemical reaction from the perspectives of the chemistry textbooks used in the 19th, the 20th and the 21st centuries in Slovakia. These findings could be useful in the process of comparison between scientific and pedagogical knowledge in this branch not only in neighbouring countries, even in the broaden European or worldwide content.
La elección de segunda persona y la construcción de identidades contextuales en el discurso radiofónico de una comunidad peninsular
Authors: Aijón Oliva Miguel Á. 
Published: October 2019

Resumen
En este trabajo se analizan las pautas de elección entre los cuatro paradigmas gramaticales de segunda persona del español peninsular (los prototípicos, representados por los pronombres tú y vosotros, y los desplazados, por usted y ustedes), cuando se usan para indexar a interlocutores específicos, en un corpus de discurso radiofónico de la ciudad española de Salamanca. Se plantea la hipótesis de que los fundamentos cognitivos de las personas gramaticales se proyectan en su potencial sociopragmático como recursos para la configuración de identidades contextuales. Tras clasificar a los participantes del corpus en cuatro tipos de identidad socioprofesional recurrentes en la comunicación radiofónica (locutores, personajes públicos, políticos y hablantes particulares), se calculan las frecuencias con que cada tipo dirige y recibe cada tratamiento de segunda persona. El análisis cuantitativo constituye un punto de partida para la observación cualitativa de los contextos de uso. Tras este se concluye que la elección entre las cuatro formas de segunda persona combina los parámetros sociopragmáticos de cercanía-distancia (que afecta a la elección entre formas prototípicas y desplazadas) e individualidad-grupalidad (que afecta a la elección entre formas singulares y plurales). Tales parámetros remiten, a su vez, al continuum discursivo-cognitivo entre la subjetividad y la objetividad.
Metformin suppresses the proliferation and invasion through NF-kB and MMPs in MCF-7 cell line
Authors: Besli Nail , Yenmis Guven , Tunçdemir Matem , Yaprak Sarac Elif , Doğan Sibel , Solakoğlu Seyhun , Kanigur Sultuybek Gönül 
Published: October 2019

Abstract

Objective
MCF-7 cells, a breast cancer cell line, are used for experiments of estrogen receptor (ER)-positive breast cancer and many sub-clones representing different classes of ER-positive tumors. We aimed to determine the efficacy of metformin, a potential anti-cancer agent, on the cell proliferation, and the expressions of NF-kB (p65), MMP-2 and MMP-9 in MCF-7 cell line.

Materials and methods
MCF-7 cells (human breast adenocarcinoma) were treated with elevating doses of metformin (0–50 mM) for 24 h. The anti-proliferative effect of metformin was studied by BrdU proliferation assay, and the expression levels of NF-kB (p65), MMP-2 and MMP-9 were analyzed by immunocytochemical staining.

Results
The percentage of cell proliferation was reduced significantly by 10 and 50 mM doses of metformin (p < 0.001). The expression levels of nuclear NF-kB (p65), MMP-9 and MMP-2 were considerably reduced in 50 mM metformin treated cells while the expression of cytoplasmic NF-kB (p65) elevated compared to control group (p < 0.05). Ten millimolar metformin also reduced expression of MMP-9 significantly (p < 0.05).

Conclusion
Metformin may act on the proliferation, and the processes of invasion and metastasis of MCF-7 cells through blocking NF-kB, which is intensely expressed in breast cancer cells, and through diminishing the expression of MMP-2 and MMP-9 significantly.
Komplementärwährungen als Instrument der Regionalentwicklung
Authors: Degens Philipp 
Published: October 2019

ZusammenfassungDer Beitrag zeigt, wie Komplementärwährungskreisläufe zur lokalen Daseinsvorsorge und Regionalentwicklung beitragen können. Komplementärwährungen können im Kleinen zu einer besseren Distribution von Ressourcen führen und damit, wenn auch begrenzt, marktschaffende, markterweiternde oder markterhaltende Funktionen innehaben. Sie sind darüber hinaus ein Mittel der Vergemeinschaftung und generieren Sozialkapital. Außerdem können sie nicht-marktliche Tauschbeziehungen stärken und zu einer gesteigerten Wertschätzung von Unterstützungsleistungen auf Gegenseitigkeit beitragen.
Specific Residues in a Purine Transporter Are Critical for Dimerization, ER Exit, and Function
Authors: Kourkoulou Anezia , Grevias Pothos , Lambrinidis George , Pyle Euan , Dionysopoulou Mariangela , Politis Argyris , Mikros Emmanuel , Byrne Bernadette , Diallinas George 
Published: October 2019

Abstract
Transporters are transmembrane proteins that mediate the selective translocation of solutes across biological membranes. Recently, we have shown that specific interactions with plasma membrane phospholipids are essential for the formation and/or stability of functional dimers of the purine transporter UapA, a prototypic eukaryotic member of the ubiquitous nucleobase ascorbate transporter (NAT) family. Here, we provide strong evidence that distinct interactions of UapA with membrane lipids are essential for ab initio formation of functional dimers in the ER, or ER exit and further subcellular trafficking. Through genetic screens, we identify mutations that restore defects in dimer formation and/or trafficking. Suppressors of defective dimerization restore ab initio formation of UapA dimers in the ER. Most of these suppressors are located in the movable core domain, but also in the core-dimerization interface and in residues of the dimerization domain exposed to lipids. Molecular dynamics suggest that the majority of suppressors stabilize interhelical interactions in the core domain and thus assist the formation of functional UapA dimers. Among suppressors restoring dimerization, a specific mutation, T401P, was also isolated independently as a suppressor restoring trafficking, suggesting that stabilization of the core domain restores function by sustaining structural defects caused by the abolishment of essential interactions with specific lipids. Importantly, the introduction of mutations topologically equivalent to T401P into a rat homolog of UapA, namely rSNBT1, permitted the functional expression of a mammalian NAT in Aspergillus nidulans. Thus, our results provide a potential route for the functional expression and manipulation of mammalian transporters in the model Aspergillus system.
Position Effects Influence Transvection inDrosophila melanogaster
Authors: King Thomas D , Johnson Justine E , Bateman Jack R 
Published: October 2019

AbstractTransvection is a form of gene regulation the depends on interactions between regulatory elements on separate chromosomes. In one form of transvection that is prevalent in Drosophila, an enhancer can act...Transvection is an epigenetic phenomenon wherein regulatory elements communicate between different chromosomes in trans, and is thereby dependent upon the three-dimensional organization of the genome. Transvection is best understood in Drosophila, where homologous chromosomes are closely paired in most somatic nuclei, although similar phenomena have been observed in other species. Previous data have supported that the Drosophila genome is generally permissive to enhancer action in trans, a form of transvection where an enhancer on one homolog activates gene expression from a promoter on a paired homolog. However, the capacity of different genomic positions to influence the quantitative output of transvection has yet to be addressed. To investigate this question, we employed a transgenic system that assesses and compares enhancer action in cis and in trans at defined chromosomal locations. Using the strong synthetic eye-specific enhancer GMR, we show that loci supporting strong cis-expression tend to support robust enhancer action in trans, whereas locations with weaker cis-expression show reduced transvection in a fluorescent reporter assay. Our subsequent analysis is consistent with a model wherein the chromatin state of the transgenic insertion site is a primary determinant of the degree to which enhancer action in trans will be supported, whereas other factors such as locus-specific variation in somatic homolog pairing are of less importance in influencing position effects on transvection.
Tripanosomiasis canina en Madre de Dios, Perú
Authors: Rojas Jaimes Jesús Eduardo , Tineo Villafuerte Edwin , Lope Huaman Roberto 
Published: October 2019

El objetivo del presente estudio fue reportar la presencia de Trypanosoma sp en una muestra de sangre de un perro en Madre de Dios, Perú. El perro era residente del distrito de Las Piedras y al ser atendido en un centro médico veterinario fue reportado con diagnóstico presuntivo de ehrlichiosis. Se tomó una muestra de sangre y el frotis fue coloreado con Wright, donde se observó los tripomastigotes de Trypanosoma sp. No se realizaron cultivos y estudios moleculares debido a que la muestra se contaminó. Este es el primer reporte de tripanosomiasis canina en Madre de Dios.
El objetivo del presente estudio fue reportar la presencia de Trypanosoma sp en una muestra de sangre de un perro en Madre de Dios, Perú. El perro era residente del distrito de Las Piedras y al ser atendido en un centro médico veterinario fue reportado con diagnóstico presuntivo de ehrlichiosis. Se tomó una muestra de sangre y el frotis fue coloreado con Wright, donde se observó los tripomastigotes de Trypanosoma sp. No se realizaron cultivos y estudios moleculares debido a que la muestra se contaminó. Este es el primer reporte de tripanosomiasis canina en Madre de Dios.
Transient Magnetohydrodynamic Free Convective Heat and Mass Transfer Flow with Thermophoresis past a Radiate Inclined Permeable Plate in the Presence of Variable Chemical Reaction and Temperature Dependent Viscosity
Authors: Alam M. S. S. , Rahman M. M. M. , Sattar M. A. A. 
Published: October 2019

In the present study, an analysis is carried out to investigate the effects of variable chemical reaction, thermophoresis, temperature-dependent viscosity and thermal radiation on an unsteady MHD free convective heat and mass transfer flow of a viscous, incompressible, electrically conducting fluid past an impulsively started infinite inclined porous plate. The governing nonlinear partial differential equations are transformed into a system of ordinary differential equations, which are solved numerically using a sixth-order Runge-Kutta integration scheme with Nachtsheim-Swigert shooting method. Numerical results for the non-dimensional velocity, temperature and concentration profiles as well as the local skin-friction coefficient, the local Nusselt number and the local Stanton number are presented for different physical parameters. The results show that variable viscosity significantly increases viscous drag and rate of heat transfer. The results also show that higher order chemical reaction induces the concentration of the particles for a destructive reaction and reduces for a generative reaction.
A Modified Holling-Tanner Model in Stochastic Environment
Authors: Maiti A. , Pathak S. 
Published: October 2019

Recently, a modified version of the so called Holling-Tannermodel isintroduced in the ecological literature. A detailed account of the deterministic dynamicsof this model is presented. The growth rates of the prey and predator are then perturbedby Gaussian white noises to take into account the effect of fluctuating environment. Theresulting stochastic model is cultured by the technique of statistical linearization andcriteria for non-equilibrium fluctuation and stability arederived. Numerical simulationsare carried out. The implications of our analytical findingsare addressed critically.
Transient Laminar MHD Free Convective Flow past a Vertical Cone with Non-Uniform Surface Heat Flux
Authors: Pullepu Bapuji , Chamkha A. J. J. 
Published: October 2019

Numerical solution of unsteady laminar free convection from an incompressible viscous fluid flow past a vertical cone with non-uniform surface heat flux qw(x) = axm varying as a power function of the distance from the apex of the cone (x = 0) in the presence of a transverse magnetic field applied normal to the surface is considered. The dimensionless governing coupled partial differential boundary layer equations are formulated and solved numerically using an efficient and unconditionally stable finite-difference scheme of the Crank-Nicolson type. The numerical results are validated by comparisons with previously published work and are found to be in excellent agreement. The velocity and temperature fields have been studied for various combinations of physical parameters (Prandtl number Pr, exponent and magnetic parameter M). The local as well as the average skin-friction parameter and the Nusselt number are also presented and analyzed graphically.
The historicity of music in Hegel in face of Schoenberg’s twelve-tone music
Authors: Kurle Adriano Bueno 
Published: October 2019

In this paper, I consider how it would be possible to think about the historicity of music through Hegel’s thought. I will compare Hegel’s idea with a historical event that is considered relevant in the history of music: Schoenberg’s twelve-tone music, taken here also as a model of immanent negation and Aufhebung of tonal system in music. Furthermore, I will take Schoenberg’s twelve-tone music as an instance and wonder about the role of music and its history in the sociocultural formation (or, in Hegelian words, in the absolute Geist). I divide the exposition into four parts, namely: (1) the position of art before the absolute Geist; (2) a general exposition of art in the Lectures on the Philosophy of Art, placing music as a singular form of art; (3) an analysis of the characteristics of music in Hegel and (4) the comparison with Schoenberg’s twelve-tone music. I conclude that music, as an artistic form, brings the form of an internal feeling of a social subjectivity – a way to feel and understand sounds that transforms itself through history; in turn, both music and the form of an internal feeling of social subjectivity, have a historicity that can be understood as developed dialectically. Along these lines, the beginning of the Twentieth Century in music reveals, through Schoenberg and other composers, new intuitive configurations of this social subjectivity.
O senso comum e a filosofia
Authors: Schöpke Regina 
Published: October 2019

Ainda que pouco conhecido e estudado em nossa época, o filósofo neokantiano russo, de origem greco-alemã, Afrikan Spir, produziu uma obra original que teve repercussões importantes em seu tempo. Nascido um pouco antes de Nietzsche, em 1837, Spir será citado por ele, sobretudo, no que se refere às questões que envolvem o caráter transcendental do conhecimento. Afinal, como Kant, Spir defende que o mundo nos aparece mediado por nossa sensibilidade; mediado e organizado pelas estruturas “a priori” do conhecimento, ou seja, do mundo recebemos apenas impressões sensoriais, qualidades sensíveis, cabendo ao sujeito cognoscente ordená-las a partir de suas intuições. No entanto, na intitulada filosofia crítica de Spir, o único e o verdadeiro, à priori, é o “princípio de identidade”, que seria o responsável pela síntese do conhecimento, seja de nós mesmos, seja dos objetos do mundo. Em poucas palavras, segundo Spir, e contestando o senso comum e o próprio objetivismo científico, não existe nada que seja estável e absoluto fora de nós. Todo o absoluto diz respeito ao pensamento. Mesmo o “eu” nada mais é do que uma síntese operada pela consciência.
Comparison of DNA extraction methods from Geranium (Geraniaceae)
Authors: Esfandani-Bozchaloyi S. , Sheidai M. , Kalalegh M. Hassanzadeh 
Published: October 2019

The genus Geranium (Geraniaceae); with about 320 species throughout the temperate regions, is chemically characterised by the presence of tannins, flavonoids, anthocyanins and essential oils which interfere with the extraction of pure genomic DNA. It is necessary to optimise the extraction protocols to reduce the effects of the presence of these compounds to the lowest level.
The present study compares the plant genomic DNA extraction Kit (DNP™ Kit), CTAB DNA extraction method by Murray and Thompson and Sahu et al., from the extracting DNA point of view Geranium species. The results showed significant differences in DNA contents between the three methods. Quantity and quality of extracted genomic DNAs were compared by employing the spectrophotometer, Nano-Drop, agarose gel electrophoresis, and polymerase chain reaction (PCR) methods and molecular marker such as (ITS and trnL-F) and ISSR. The method of Sahu et al., provided the best results (200 ng/µL) in terms of quantity and quality of DNA, therefore, this method was taken and optimised for DNA extraction. Our results proposed that this method could be effective for plants with same polysaccharides, proteins and polyphenols components. The advantage of this method is that it omits the use of liquid nitrogen and toxic phenols which are expensive. The success of this method in obtaining high-quality genomic DNA has been demonstrated in the Geranium species group and the reliability of this method has been discussed.
MODERN STUDENT VALUE-SEMANTIC ATTITUDE FORMATION TOWARDS CHOSEN OCCUPATION IN UNIVERSITY EDUCATIONAL ENVIRONMENT
Authors: S. Krylova Yulia , G. Artemova Evgeniya , A. Bodina Elena , N. Telysheva Natalia , L. Knyazeva Galina , V. Pokrovskaya Svetlana , N. Charikova Irina , G. Kashina Svetlana 
Published: October 2019

Purpose of the study: The purpose of the article is to substantiate the need for students’ value-semantic attitude formation towards the chosen occupation based on the University educational environment resources activation. This attitude, as practical experience proves, allows more successfully carry out occupational activities, strive for self-improvement of labor skills, and permanently extrapolates this attitude to the profession in one’s own life. Methodology: Research on testing models of value-semantic attitude’s formation to the profession among the students was conducted in the period from 2009 to 2018. Experimental research covered 395 people, including 339 students and 56 teachers. The following techniques and tests contributing to diagnostics were used for the study: the personality's strong-willed organization, the training motivation, educational achievements and need for them, valuable orientation-based unity of the group, the attitude to specific types of occupational activity. Results: The article clarifies the concept of value-semantic attitude to the profession, characterizes the educational environment of the University in the context of this problem, presents the functions and components of the axiological approach aimed at the development of the University educational environment; defines the pedagogical conditions under which students’ value-semantic attitude to the chosen profession will be qualitatively formed. The article reveals the possibilities of simulating such an educational environment. The article describes the results of the interrelated components’ implementation of the developed model for value-semantic relations’ formation, implemented for many years in educational institutions of higher education. Applications of this study: The possibility of educational courses, methods, forms and training technologies adaptation to the educational environment of universities, regardless of their profile orientation is described. Novelty/Originality of this study: The article proposes adapted and developed diagnostic methods; presents the developed criteria and levels to identify the formation of value-semantic attitude to the profession among the students.
Relationships Between Adolescents' Knowledge, Attitudes, and Fears Related to HIV/AIDS
Authors: Mahat Ganga 
Published: October 2019

AimsTo explore adolescents' knowledge, attitudes, and fears of HIV/AIDS and to examine relationships between these variables and demographic variables among female adolescents.BackgroundHIV/AIDS is one of the major public health concerns all around the world, including India. Although the prevalence of HIV infection is low, it is still considered a high risk among Indian youth because of low and inaccurate HIV/AIDs knowledge, low condom usage and unsafe sexual practice, and lack of access to information.MethodsA descriptive correlational design was used in this study. A convenience sample of 150 adolescents attending one of the girls' high schools completed demographic and HIV/AIDS knowledge and attitude questions in June 2018. The data was analyzed using descriptive statistics and Pearson-product–moment correlation coefficients.ResultsThe majority of adolescents had a moderate degree of HIV/AIDS knowledge, however; many of them still had misconceptions about modes of HIV/AIDS transmission and prevention of HIV infection. Most of the adolescents had fairly positive attitudes and beliefs toward people with HIV/AIDS. About three-fourths of the participants were afraid of getting HIV/AIDS. The total HIV/AIDS knowledge was positively correlated with age (r = 0.288, p < .000), fathers' education (r = 0.235, p < .003), fear of getting HIV infection (r = 0.222, p < .005), and discussion about sexually transmitted diseases with parents (r = 0.195, p < .01).ConclusionThe findings indicate a need for educating students on HIV/AIDS, which can be accomplished by incorporating information regarding HIV transmission and prevention into their school curriculum. School-based educational program could be beneficial.
Local Adjuvant Substances Following Curettage of Bone Tumors
Authors: Bickels Jacob , Campanacci Domenico A. 
Published: October 2019

➤ Benign and low-grade malignant tumors of bone that require intralesional excision by means of curettage are often also treated by a variety of local adjuvant substances for the destruction of any microscopic disease remaining within the tumoral cavity.➤ Concerns about the efficacy and associated morbidity of these adjuvants have often been raised, and the indication for their use is still being debated.➤ The superiority of a single adjuvant has not been documented.➤ Polymethylmethacrylate is not considered a local adjuvant to curettage but rather a mechanical reinforcement of the tumoral cavity.➤ Meticulous tumor curettage and high-speed burring through wide exposure of the tumoral cavity is apparently the key factor in efficacious local tumor control.