bluuebunny/crossref_metadata_2025_split huggingface dataset:
High‐Throughput Discovery of Substrate Peptide Sequences for E3 Ubiquitin Ligases Using a cDNA Display Method
Authors: Tamagawa Kenwa , Campbell Robert E. , Terai Takuya 
Published: November 2024

AbstractCells utilize ubiquitin as a posttranslational protein modifier to convey various signals such as proteasomal degradation. The dysfunction of ubiquitylation or following proteasomal degradation can give rise to the accumulation and aggregation of improperly ubiquitylated proteins, which is known to be a general causation of many neurodegenerative diseases. Thus, the characterization of substrate peptide sequences of E3 ligases is crucial in biological and pharmaceutical sciences. In this study, we developed a novel high‐throughput screening system for substrate peptide sequences of E3 ligases using a cDNA display method, which enables covalent conjugation between peptide sequences and their corresponding cDNA sequences. First, we focused on the MDM2 E3 ligase and its known peptide substrate as a model to establish the screening method, and confirmed that cDNA display method was compatible with in vitro ubiquitylation. Then, we demonstrated identification of MDM2 substrate sequences from random libraries to identify a novel motif (VKFTGGQLA). Bioinformatics analysis of the hit sequences was performed to gain insight about endogenous substrate proteins.
High malaria parasitemia in an Italian man and severe delayed hemolytic anemia
Authors: Lanzafame Massimiliano , Mori Giovanni , Lattuada Emanuela , Scarparo Claudio 
Published: November 2024

Key Clinical MessageParenteral artesunate has become the choice treatment of cases of high parasitemia malaria. We report the case of an Italian man with a high malaria parasitemia who promptly improved with intravenous artenusate, but who developed a delayed severe hemolytic anemia associated with intravenous artesunate treatment, requiring blood trasfusions.
Modulating the coordination environment of cobalt porphyrins for enhanced electrochemical nitrite reduction to ammonia
Authors: Han Jingwei , Sun Hai , Tian Fengkun , Zhang Wenwen , Zhang Zonghang , She Ping , Qin Jun‐Sheng , Rao Heng 
Published: November 2024

AbstractElectrocatalytic reduction of nitrate pollutants to produce ammonia offers an effective approach to realizing the artificial nitrogen cycle and replacing the energy‐intensive Haber‐Bosch process. Nitrite is an important intermediate product in the reduction of nitrate to ammonia. Therefore, the mechanism of converting nitrite into ammonia warrants further investigation. Molecular cobalt catalysts are regarded as promising for nitrite reduction reactions (NO2−RR). However, designing and controlling the coordination environment of molecular catalysts is crucial for studying the mechanism of NO2−RR and catalyst design. Herein, we develop a molecular platform of cobalt porphyrin with three coordination microenvironments (Co‐N3X1, X = N, O, S). Electrochemical experiments demonstrate that cobalt porphyrin with O coordination (CoOTPP) exhibits the lowest onset potential and the highest activity for NO2−RR in ammonia production. Under neutral, non‐buffered conditions over a wide potential range (−1.0 to −1.5 V versus AgCl/Ag), the Faradaic efficiency of nearly 90% for ammonia was achieved and reached 94.5% at −1.4 V versus AgCl/Ag, with an ammonia yield of 6,498 μg h−1 and a turnover number of 22,869 at −1.5 V versus AgCl/Ag. In situ characterization and density functional theory calculations reveal that modulating the coordination environment alters the electron transfer mode of the cobalt active center and the charge redistribution caused by the break of the ligand field. Therefore, this results in enhanced electrochemical activity for NO2−RR in ammonia production. This study provides valuable guidance for designing adjustments to the coordination environment of molecular catalysts to enhance catalytic activity.
Water Quality Evaluation With Entropy Weight‒Grey Correlation Technique for Fishing Operation Area in Tianjin, China
Authors: Yuan Hui , Han Shaoqiang , Tian Ye , Zhang Ying , Bo Zhe , Liu Jiahong 
Published: November 2024

ABSTRACTThe discharge of domestic sewage and waste oil from fishery activities can cause adverse impacts on coastal seawater in bays and estuaries. The Bohai Sea near Tianjin features strong water closure and weak self‐purification ability. The objective evaluation and analysis of pollution characteristics are crucial for assessing the quality of coastal seawater. This evaluation is essential for the sustainable development of traditional marine fisheries and serves as a powerful tool for government decision‐making. To this end, we constructed an entropy weight‒grey correlation technique for order preference by similarity to an ideal solution (TOPSIS) model (EWGCT) based on the traditional TOPSIS evaluation approach to evaluate the water quality of five fishing ports and operation areas. The spatial distribution characteristics, pollution characteristics, and key impactful indicators were identified via a geographic information system (GIS) and multivariate statistical analysis. The results show that (1) the EWGCT has the ability to rank water quality universally and evaluate it based on the comprehensive impact of all pollution factors; (2) petroleum (PETRO) was affected mainly by the waste oil discharged, and suspended solids (SS), dissolved inorganic phosphate (DIP), and fecal coliform bacteria (FCB) were affected mainly by the domestic sewage discharged; and (3) effective management of fishing vessel discharge is important for ensuring the quality of the seawater environment. By establishing the EWGCT, the reliability and rationality of the evaluation results are verified, which can provide a reference for the formulation of development strategies for coastal cities.
Comparison of Machine Learning Algorithms for Prediction of Textile Effluent Treatment Efficiency Using Anaerobic Process
Authors: Samuchiwal Saurabh , Saraswat Saurabh , Nair Vivek Kumar , Chaudhary Aman , Malik Anushree 
Published: November 2024

ABSTRACTThe prediction of pollutants removal efficiency from the generated effluent of a treatment plant is valuable and can reduce the time, sampling and energy required during performance assessment. The present study aims to predict the effect of different input parameters on the treatment efficiency of the developed microbial‐based anaerobic process for textile effluent using machine leaning algorithms. The decolourisation and chemical oxygen demand (COD) reduction of the treated effluent were predicted on the basis of the three different input parameters pH, COD and colour value of the textile wastewater. The effectiveness of different machine learning algorithms, support vector machines (SVM), random forest (RF), gradient boost regressor (GBR), AdaBoost, extreme gradient boosting (XGB) regressor and voting regressor, were evaluated based on the correlation coefficient (R2) value. The results revealed that the RF achieved the highest accuracy for decolourisation (training data R2: ∼0.85 and test data R2: ∼0.84) as well as COD reduction (training data R2: ∼0.87 and test data R2: ∼0.94) compared to the other algorithms. These results were validated experimentally, confirming that RF can be used as a tool to predict the performance efficiency of a microbial‐based treatment system.
Analyzing Extracellular Vesicle‐associated DNA Using Transmission Electron Microscopy at the Single EV‐level
Authors: Tsering Thupten , Nadeau Amélie , Rak Janusz , Burnier Julia V. 
Published: November 2024

AbstractExtracellular vesicles (EVs) play an important role in cell‐cell communication, carrying bioactive molecules including DNA. EV‐associated DNA (EV‐DNA) has created enormous interest in the field of biomarkers, particularly related to liquid biopsy. However, its analysis is challenging due to the nanoscale structure of EVs, the low abundance of EV‐DNA, and surrounding biogenetic debate. Therefore, novel protocols to enhance the accurate detection of EV‐DNA are essential to study its role in normal physiology and disease states. Here, we provide two protocols for confirming the presence of EV‐DNA from biological samples. In the first protocol, ultrathin sectioning of EVs is combined with immunogold labeling to detect the presence of double‐stranded (ds) DNA within the EV lumen using transmission electron microscopy (TEM). In the second protocol, whole‐mount EV immunogold labeling allows detailed morphological analysis of EVs and their surface‐associated DNA. Using TEM imaging, we have demonstrated that cancer‐cell‐derived individual EVs exhibit simultaneous positivity for dsDNA and the EV surface protein tetraspanin 9. We believe that this method can be used to label any proteins of interest inside as well as on the surface of EVs. This can aid in the characterization of single EVs and in the identification and verification of EV‐associated biomarkers. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: EV isolation from cell‐culture‐conditioned medium, EV embedding, ultrathin sectioning, labeling, and imagingBasic Protocol 2: Whole‐mount immunolabeling of EV‐DNA
Exploring food delivery app adoption: Corporate social responsibility and perceived product risk's influence
Authors: Prisco Anna , Sepe Fabiana , Nanu Luana , Tani Mario 
Published: November 2024

AbstractThis study examines factors influencing consumer adoption of food delivery apps during and after the COVID‐19 pandemic, focusing on the role of perceived product risk and sustainability alongside traditional behavioral predictors. Using the Theory of Planned Behavior extended with perceived product risk and sustainability components, the study employs a quantitative survey approach to assess consumer attitudes and intentions regarding food delivery app usage via PLS‐SEM. Subjective norms strongly predict the intention to use food delivery apps, while perceived product risk and sustainability concerns negatively influence adoption. This indicates that consumers valuing social and environmental issues are less likely to use these apps. This research offers a novel perspective by integrating perceived product risk and sustainability into the study of food delivery app adoption, enriching the existing literature, and providing insights on consumer behavior in the digital marketplace.
Reversible Photo‐Responsive Hydrophobic Coating Synthesized from Lignin‐Derivable Molecules on Nanocellulose Films for Packaging Applications
Authors: Sinha Roy Pallabi , Nasiri Naghmeh , Patti Antonio F. , Allais Florent , Saito Kei , Garnier Gil 
Published: November 2024

AbstractPaper‐based packaging can offer a sustainable replacement for plastics. However, paper provides a poor barrier to water, oxygen and moisture. This study presents a novel renewable lignocellulosic composite made from a hydrophobic photo‐reversible coating deposited onto a cellulose nanofiber film that has improved barrier properties and can be reprocessed. Diglycerol and lignin‐derivable aldehyde were reacted to form a tetra‐functional monomer with photo‐responsive unsaturated double bonds that can be converted to covalent cyclobutane rings to create reversibly crosslinkable network upon UV‐irradiation. The photo‐responsive compound was applied as a thin coating of thickness 2.7±0.4 μm over cellulose nanofiber (CNF) films of thickness 80±19 μm. The surface of the coated films became hydrophobic with a contact angle (CA) of 93.1±1.7° and displayed a low water vapour transmission rate (WVTR) of 16±2 g/m2/day vs. 30.7±1.5° CA and 81±11 g/m2/day WVTR for uncoated CNF films. The coated film is also oleophobic, an attractive feature for food packaging applications. The reversible photo‐reaction enables the crosslinked covalent network to be broken down to unsaturated double bonds once exposed to a higher‐energy UV irradiation, allowing reprocessing and recycling. The novel coating was developed using a sustainable green synthesis method (process simple E factor 0.9).
How Emergent Vegetation Patch Shapes Affect Flow Structure in Open Channel Environments
Authors: Anjum Naveed , Pasha Ghufran Ahmed , Ashraf Jawad , Ghani Usman , Abbas Fakhar Muhammad , Rahman Md Abedur 
Published: November 2024

ABSTRACTVegetation patches, which naturally occur in a variety of patterns, have an impact on the flow dynamics and geometry of rivers. There have been prior studies on vegetation patches, but none have examined the impact of differently shaped vegetation patch, i.e., circle, square and rectangle, and the comparison of these varying patch shapes from an ecological point of view. Hence, a computational fluid dynamics (CFD) technique using FLUENT has been employed in the present research to evaluate the flow dynamics and turbulence characteristics around emergent vegetation patches of various forms in an open channel. The results show that the shape of vegetation patches had a significant impact on the approaching flow, with circular patch offering the highest flow resistance among all other configurations. Flow shedding behaviour behind the patch was also prominent in the case of circular patch shape with a formation of large Kármán vortex street with vortex sizes up to 1.5 times the patch diameter. In the downstream region of vegetation patch, a reduction of 25%–30% in the mean streamwise velocity was observed for circular patch, whereas a 15% decrease was observed for rectangular and square patches. The outcomes of this study found that flow turbulence and stability in the open channel are significantly influenced by vegetation patch shape. This research reveals that the shape of vegetation patches, particularly circular configuration, significantly influences flow dynamics, thereby enhancing habitat complexity in riverine ecosystems. These findings are vital for developing informed river restoration and management strategies that support biodiversity and promote ecological resilience.
Effect of Mesembryanthemum crystallinum and its derived D‐pinitol on HMG‐CoA reductase and tyloxapol‐induced hyperlipedemia
Authors: Choi Jun‐Hui , Park Se‐Eun , Kim Seung 
Published: November 2024

AbstractSeveral previous research indicate that treating Mesembryanthemum crystallinum may aid in reducing adipogenesis and triacylglycerol level and improving hyperglycemia and diabetes. Therefore, the present study was designed to evaluate the effectiveness of M. crystallinum extract (MCE) in combating obesity and lowering fat/lipid/cholesterol levels. The study aimed to investigate the molecular docking model targeting 3‐hydroxy‐3‐methylglutaryl‐CoA reductase (HMGCR) using MCE‐derived d‐pinitol or atorvastatin, an inhibitor of HMGCR. In this study, histological alterations in the liver of tyloxapol‐induced hyperlipidemia (TIH) model, hyperlipidemia‐related markers in serum, HMGCR activity, and cell viability in HepG2 cells were analyzed. Our findings revealed that tyloxapol treatment significantly led to increased hyperlipidemia‐related indicators and cardiovascular‐associated risk indices. However, MCE effectively mitigated tyloxapol‐induced hepatic fat accumulation and an increase in cholesterol levels. This was achieved through improvements in metabolic parameters, ultimately ameliorating TIH. These beneficial results suggest that MCE may be a strong potential for the treatment of hyperlipidemia‐related diseases.
Recent advances on the impact of protumorigenic dietary‐derived bacterial metabolites on the intestinal stem cell
Authors: Holmes Carys , Illingworth Charlotte H. , Parry Lee 
Published: November 2024

AbstractThe links between diet, microbiome, immunity, and colorectal cancer are well established. The metabolite output of the microbiome, which has a large influence over host health and disease, is related to the composition of the diet. These metabolites subsequently impact on immune and intestinal epithelial either directly or indirectly via production of secondary metabolites. Here we summarize the latest findings and briefly discuss their potential for managing disease risk.
A2B2‐zinc(II)porphyrin/divinylbenzene Copolymer as Efficiently Bifunctional Catalyst for Cycloaddition of CO2 with Epoxides
Authors: Yang Yong , Yan Peng , Wang Dan , Xie Fang , Song Dengmeng , Wang Ning , Li Jun 
Published: November 2024

AbstractIn this work, the A2B2‐type Zn‐porphyrin (ZnPorI2) featuring vinyl and imidazolium groups was utilized to prepare porous organic polymers (POPs). The vinyl group served as the linking unit for copolymerization with rigid divinylbenzene (DVB). The imidazolium group introduced iodide ions (I−) into ZnPorI2/nDVB (n=30, 50) as Lewis base. By fine‐tuning the ZnPorI2 to DVB ratio, it is possible to optimize the porosity, morphology, and content of Zn‐porphyrin. Owing to the synergistic effect of Zn2+ and I−, the as‐prepared ZnPorI2/30DVB exhibited excellent catalytic activities in the cycloaddition of carbon dioxide with epoxides.
Homobarrelenones: Versatile Building Blocks for the Construction of Novel and Structurally Diverse Scaffolds
Authors: Lucero Valeria , do Carmo Hugo , Fernández Gabriel , Cabrera Mauricio , Eugui Macarena , Moyna Guillermo 
Published: November 2024

AbstractWe describe the derivatization of tropolone–based homobarrelenones through simple transformations, including catalytic hydrogenations, base‐catalyzed solvolysis, and hydride reductions. Despite being structurally complex and highly functionalized, the novel compounds had no significant cytotoxicity in sperm motility assays, underscoring the importance of Michael acceptor moieties in the biological activity of this class of compounds.
First‐choice hormonal therapies for children with infantile epileptic spasms syndrome in South Asia: A network meta‐analysis of randomized controlled trials
Authors: Devi Nagita , Madaan Priyanka , Kandoth Nidhun , Lal Parth , Sahu Jitendra Kumar , Bansal Dipika 
Published: November 2024

AbstractConsidering the peculiar challenges with infantile epileptic spasms syndrome (IESS) in South Asia and a wide variation in the usage of hormonal therapies, we compared the efficacy and safety of various hormonal therapies for children with IESS in South Asia. We searched PubMed, Embase, Scopus, and Web of Science databases from the inception until April 2024. We included only randomized clinical trials (RCTs) evaluating the efficacy and safety of hormonal therapies for IESS in the South Asian region. Complete cessation of epileptic spasms (ES), electro‐clinical response, and time taken to be spasm‐free at 2 or 6 weeks of therapy were efficacy outcomes, while the occurrence of adverse events was the safety outcome. Effect estimates were reported as odds ratio (OR) or mean difference (MD) with 95% confidence intervals (CI) and Cochrane risk of bias 2.0 (ROB 2.0) used for quality assessment of each study. The surface under the cumulative ranking curve (SUCRA) was used to rank the different therapies and reported as a p‐score ranging from 0 to 1. Of 747 citations, nine RCTs comprising 566 children with IESS were included. After 2‐week treatment, dexamethasone (OR: 6.72; 95% CI: 1.47, 30.72), adrenocorticotropic hormone therapy (ACTH) high dose (HD) (OR: 5.30; 95% CI: 1.05, 26.91), and prednisolone HD (OR: 2.41; 95% CI:1.07, 5.46) had shown significantly greater efficacy for cessation of EScompared with ACTH low dose (LD). Similarly, for electroclinical response, dexamethasone (OR: 9.63; 95% CI: 1.99, 46.70) and prednisolone HD (OR: 3.46; 95% CI: 1.38, 8.68) had greater efficacy compared with ACTH LD. Safety outcomes revealed that hypertension was significantly less common with ACTH LD and prednisolone HD as compared with ACTH HD. This study provides quality evidence on preferred first‐choice hormonal therapy for managing IESS in South Asia. ACTH HD, dexamethasone, and prednisolone HD are the most effective hormonal therapy options with dose‐dependent therapeutic efficacy.Plain Language SummaryThis study provides insights into the selection of first‐line hormonal therapies among the various treatments for managing infantile epileptic spasms syndrome (IESS) in South Asia. The study findings suggested that the effectiveness of these therapies is dose‐dependent, with high doses of ACTH, dexamethasone, and prednisolone being the most effective for achieving cessation of epileptic spasms.
From caretaking to transaction: Women politicians, motherhood, and political authority in Kenya
Authors: Kilimo Miriam Jerotich 
Published: November 2024

AbstractFeminist scholars have noted how motherhood opens avenues for women to access political authority. For African and Black women, scholars have noted how presenting themselves as mothers in the political sphere allows them to capitalize on an identity that traditionally allowed them access to power. However, these perspectives on motherhood and politics privilege a perspective on motherhood that deems the role as selfless and sacrificial. In this article, I propose moving beyond the caretaking dimensions of motherhood when analyzing how women politicians utilize motherhood. Drawing on evidence from research among Kenyan women politicians between 2017 and 2020, I argue that political motherhood often emerges as a transaction. Rather than interpreting the actions of women politicians as an extension of maternalistic impulses into the political realm, I illustrate how women politicians present the benefits of motherhood in exchange for citizens’ votes. By diversifying the meanings of political motherhood beyond caretaking and communal roles, this article counters perspectives that assume women politicians are less corrupt or are the victims of patriarchal politics. Instead, by foregrounding the transactional dimensions of political motherhood, this article illustrates how women politicians strategically use gendered identities to build political authority.
Large‐scale marine protected areas and imaginaries of progress in ocean governance
Authors: Montana Jasper , Hartman Davies Oscar 
Published: November 2024

AbstractLarge‐scale marine protected areas (LSMPAs) are an increasingly important feature of global conservation as countries strive to meet international commitments to protect 30% of all land and sea areas by 2030. In this paper, we contribute to current interest in the imaginaries that underpin environmental governance. Drawing together work on spatial and sociotechnical imaginaries, we examine how ocean imaginaries get bound up with the rise of large, protected areas in the ocean. We develop a typology of three ocean imaginaries associated with LSMPAs, which is elaborated through an empirical analysis of the political discourse that surrounded the designation of 17 LSMPAs since 2010. We examine extracts of government statements, speeches and press releases predominantly in news article sources and government websites to consider how these ocean imaginaries are institutionally stabilised and aligned with advances in science and technology. Our analysis reinforces an understanding that the kinds of spatial imaginaries that are created for environmental governance shape and are shaped by policy and management strategies. We also find that both visions of ocean spaces and the social worlds that perceive them can be multiple. We contend that research and policy need to recognise LSMPAs and other area‐based conservation measures as more‐than‐technical pursuits, and harness geographic scholarship to consider and enable a multiplicity of imaginaries in exploring options for environmental governance.