bluuebunny/crossref_metadata_2025_split huggingface dataset:
ROP INTERACTIVE PARTNER b interacts with RACB and supports fungal penetration into barley epidermal cells
Authors: McCollum Christopher , Engelhardt Stefan , Weiss Lukas , Hückelhoven Ralph 
Published: August 2019

AbstractRHO of Plants (ROP) G-proteins are key components of cell polarization processes in plant development. The barley (Hordeum vulgare) ROP protein RACB, is a susceptibility factor in the interaction of barley with the barley powdery mildew fungus Blumeria graminis f.sp. hordei (Bgh). RACB also drives polar cell development, and this function might be coopted during formation of fungal haustoria in barley epidermal cells. In order to understand RACB signaling during the interaction of barley with Bgh, we searched for potential downstream interactors of RACB. Here, we show that ROP INTERACTIVE PARTNER b (RIPb, synonym: INTERACTOR OF CONSTITUTIVE ACTIVE ROP b; ICRb) directly interacts with RACB in yeast and in planta. Over-expression of RIPb supports susceptibility of barley to Bgh. RIPb further interacts with itself at microtubules. However, the interaction with activated RACB takes place at the plasma membrane. Both, RIPb and RACB are recruited to the site of fungal attack around the neck of developing haustoria suggesting locally enhanced ROP activity. We further assigned different functions to different domains of the RIPb protein. The N-terminal coiled-coil CC1 domain is required for microtubule localization, while the C-terminal coiled-coil CC2 domain is sufficient to interact with RACB and to fulfill a function in susceptibility at the plasma membrane. Hence, RIPb appears to be localized at microtubules and is then recruited by activated RACB for a function at the plasma membrane during formation of the haustorial complex.One Sentence summaryRIPb acts downstream of the powdery mildew susceptibility factor RACB of barley and influences susceptibility
Biallelic TANGO1 mutations cause a novel syndromal disease due to hampered cellular collagen secretion
Authors: Lekszas Caroline , Foresti Ombretta , Raote Ishier , Liedtke Daniel , König Eva-Maria , Nanda Indrajit , Vona Barbara , De Coster Peter , Cauwels Rita , Malhotra Vivek , Haaf Thomas 
Published: August 2019

AbstractThe transport and Golgi organization 1 (TANGO1) family proteins have been shown to play pivotal roles in the secretory pathway. Full length TANGO1 is a transmembrane protein localised at endoplasmic reticulum exit sites (ERES), where it binds bulky cargo within the ER lumen and recruits membranes from the ER Golgi intermediate compartment (ERGIC) to create an exit route for their export. Tango1 knockout mice display a global collagen secretion defect and perinatal lethality. Here we report the first TANGO1-associated syndrome in humans, which mainly manifests in a collagenopathy. A synonymous substitution that results in exon 8 skipping in most mRNA molecules, ultimately leading to a truncated TANGO1 protein was identified as the disease-causing mutation. The four homozygously affected sons of a consanguineous family display severe dentinogenesis imperfecta, short stature, various skeletal abnormalities, insulin-dependent diabetes mellitus, sensorineural hearing loss, and mild intellectual disability. Functional studies in HeLa and U2OS cells revealed that the corresponding truncated TANGO1 protein is dispersed in the ER and its expression in cells with intact endogenous TANGO1 impairs cellular collagen I secretion.
scHLAcount: Allele-specific HLA expression from single-cell gene expression data
Authors: Darby Charlotte A. , Stubbington Michael J. T. , Marks Patrick J. , Barrio Álvaro Martínez , Fiddes Ian T. 
Published: August 2019

AbstractStudies in bulk RNA sequencing data suggest cell-type and allele-specific expression of the human leukocyte antigen (HLA) genes. These loci are extremely diverse and they function as part of the major histocompatibility complex (MHC) which is responsible for antigen presentation. Mutation and or misregulation of expression of HLA genes has implications in diseases, especially cancer. Immune responses to tumor cells can be evaded through HLA loss of function. However, bulk RNA-seq does not fully disentangle cell type specificity and allelic expression. Here we present scHLAcount, a workflow for computing allele-specific molecule counts of the HLA genes in single cells an individualized reference. We demonstrate that scHLAcount can be used to find cell-type specific allelic expression of HLA genes in blood cells, and detect different allelic expression patterns between tumor and normal cells in patient biopsies. scHLAcount is available at https://github.com/10XGenomics/scHLAcount.
Interplay of disordered and ordered regions of a human small heat shock protein yields an ensemble of “quasi-ordered” states
Authors: Clouser Amanda F. , Baughman Hannah E.R. , Basanta Benjamin , Guttman Miklos , Nath Abhinav , Klevit Rachel E. 
Published: August 2019

ABSTRACTSmall heat shock proteins (sHPSs) are nature’s “first responders” to cellular stress, interacting with affected proteins to prevent their aggregation. Little is known about sHSP structure beyond its structured α-crystallin domain (ACD), which is flanked by disordered regions. In the human sHSP HSPB1, the disordered N-terminal region (NTR) represents nearly 50% of the sequence. Here, we present a hybrid approach involving NMR, hydrogen-deuterium exchange mass spectrometry, and modeling to provide the first residue-level characterization of the NTR. The results support a model in which multiple grooves on the ACD interact with specific NTR regions, creating an ensemble of “quasi-ordered” NTR states that can give rise to the known heterogeneity and plasticity of HSPB1. Phosphorylation-dependent interactions inform a mechanism by which HSPB1 is activated under stress conditions. Additionally, we examine the effects of disease-associated NTR mutations on HSPB1 structure and dynamics, leveraging our emerging structural insights.
Antibiotic Resistance Increases Evolvability and Maximizes Opportunities Across Fitness Landscapes
Authors: Spagnolo Fabrizio , Dykhuizen Daniel E. 
Published: August 2019

AbstractAntibiotic resistance continues to grow as a public health problem. One of the reasons for this continued growth is that resistance to antibiotics is strongly selected for in the presence of antibiotics and weakly selected against after their removal. This is frequently thought to be due to the effects of compensatory mutations. However, compensatory mutations are often not found in clinically relevant strains of antibiotic resistant pathogens. Here, we conduct experimentsin vitrothat highlight the role that fine scale differences in environment play in the maintenance of populations after selection for resistance. We show that differences in the mode of growth, dictated by environmental factors, are capable of reliably changing the force and direction of selection. Our results show that antibiotic resistance can increase evolvability in environments if conditions for selection exist, selecting differentially for newly arising variation and moving populations to previously unavailable adaptive peaks.SignificanceAntibiotic resistant bacteria are a large and growing problem for public health. A major question has been why antibiotic resistant strains do not disappear when they must compete with higher fitness drug sensitive strains. Here we show that selection for antibiotic resistant strains is particularly sensitive to differences in environmental conditions and that these differences help to define the fitness landscapes upon which these populations adapt. The result is an increase in evolvability, with many adaptive peaks that drug resistant populations can explore through natural selection, making predictions of evolution difficult and selection against resistant strains improbable.
Functional characterization of fatty acyl desaturase Fads2 and Elovl5 elongase in the Boddart’s goggle-eyed goby,Boleophthalmus boddarti(Gobiidae) suggest an incapacity for long-chain polyunsaturated fatty acid biosynthesis
Authors: Soo Han-Jie , Chong Joey , Sean Lau Nyok , Ting Seng Yeat , Kei Sam Ka , Kuah Meng-Kiat , Kwang Sim Yee , Janaranjani M. , Shu-Chien Alexander Chong 
Published: August 2019

AbstractLong-chain polyunsaturated fatty acid biosynthesis, a process to convert C18 polyunsaturated fatty acids to eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) or arachidonic acid (ARA) requires the concerted activities of two enzymes, the fatty acyl desaturase (Fads) and elongase (Elovl). This study highlights the cloning, functional characterisation and tissue expression pattern of a Fads and Elovl from the Boddart’s goggle-eyed goby (Boleophthalmus boddarti), a mudskipper species widely distributed in the Indo-Pacific region. Phylogenetic analysis revealed that the cloned Fads and Elovl are clustered with other teleost Fads2 and Elovl5 orthologs, respectively. Interrogation of the genome of several mudskipper species, namelyB. pectinirostris, Periophthalmus schlosseriandP. magnuspinnatusrevealed a single Fads2 for each respective species while two elongases, Elovl5 and Elovl4 were detected. Using a heterologous yeast assay, theB. boddartiFads2 was shown to possess low desaturation activity on C18 PUFA. In addition, there was no desaturation of C20 and C22 substrates. In comparison, the Elovl5 showed a wide range of substrate specificity, with capacity to elongate C18, C20 and C22 PUFA substrates. We identified an amino acid residue in theB. boddartiElovl5 that affect the capacity to bind C22 PUFA substrate. Both genes are highly expressed in brain tissue. Among all tissues, DHA is highly concentrated in neuron-rich tissues while EPA is highly deposited in gills. Taken together, the results showed that due to disability of desaturation steps,B. boddartiis unable to biosynthesis LC-PUFA, relying on dietary intake to acquire these nutrients.
The physical environment and patient satisfaction ratings on social media: an exploratory study
Authors: Alkazemi Mariam F. , Bayramzadeh Sara , Alkhubaizi Nouf B. , Alayoub Ayman 
Published: August 2019

Purpose
The purpose of this study is to explore the role of the physical environment in patient satisfaction ratings as communicated in narratives on the social media platform such as Facebook.

Design/methodology/approach
Publicly available Facebook reviews (n = 4,502) of a reputable healthcare system in the USA were analyzed. A thematic analysis was conducted to explore architectural elements of the physical environment that play a role in patient satisfaction.

Findings
Facebook reviews were examined for the presence of design-related factors within the physical environment. Of the 627 posts (14 per cent) with relevant content, 56 involved factors related to the physical environment. The factors include: location, parking, cleanliness, privacy, waiting rooms, music and temperature. The results showed that environmental and design-related factors are part of patient satisfaction in hospitals.

Research limitations/implications
Not all Facebook reviews contain narrative information. Nevertheless, the impact of the built environment can manifest in online reviews of healthcare systems. Future patient satisfaction research should examine variables related to the built environment on social media ratings.

Practical implications
Social media feedback about the physical environment can help in understanding factors influencing patient satisfaction, which can have an implication for architectural design.

Social implications
The patient satisfaction is related to the physical environment of healthcare facilities. Some social media narratives reflect it and can be used to improve patient satisfaction.

Originality/value
Although some studies examine social media narratives on patient satisfaction, fewer studies examine these narratives in relation to the built environment. Created by a team of interdisciplinary researchers, this study provides a novel approach to examine social media ratings.
Characteristic stabilized finite element method for non-stationary conduction-convection problems
Authors: Wang Yongshuai , Mahbub Md. Abdullah Al , Zheng Haibiao 
Published: August 2019

PurposeThis paper aims to propose a characteristic stabilized finite element method for non-stationary conduction-convection problems.Design/methodology/approachTo avoid difficulty caused by the trilinear term, the authors use the characteristic method to deal with the time derivative term and the advection term. The space discretization adopts the low-order triples (i.e. P1-P1-P1and P1-P0-P1triples). As low-order triples do not satisfy inf-sup condition, the authors use the stability technique to overcome this flaw.FindingsThe stability and the convergence analysis shows that the method is stable and has optimal-order error estimates.Originality/valueNumerical experiments confirm the theoretical analysis and illustrate that the authors’ method is highly effective and reliable, and consumes less CPU time.
Learning adaptation to climate change from past climate extremes:
Authors: Aryal Jeetendra Prakash , Jat M.L. , Sapkota Tek Bahadur , Rahut Dil Bahadur , Rai Munmum , Jat Hanuman S. , Sharma P.C. , Stirling Clare 
Published: August 2019

PurposeConservation agriculture-based wheat production system (CAW) can serve as anex antemeasure to minimize loss due to climate risks, especially the extreme rainfall during the wheat production season in India. This study aims to examine whether farmers learn from their past experiences of exposure to climate extremes and use the knowledge to better adapt to future climate extremes.Design/methodology/approachThe authors used data collected from 184 farmers from Haryana over three consecutive wheat seasons from 2013-2014 to 2015-2016 and multivariate logit model to analyse the driver of the adoption of CAW as anex anteclimate risk mitigating strategies based on their learning and censored Tobit model to analyse the intensity of adoption of CAW as anex anteclimate risk mitigation strategy. Farmer’s knowledge and key barriers to the adoption of CAW were determined through focus group discussions.FindingsThe analysis shows that the majority of farmers who had applied CAW in the year 2014-2015 (a year with untimely excess rainfall during the wheat season) have continued to practice CAW and have increased the proportion of land area allocated to it. Many farmers shifted from CTW to CAW in 2015-2016.Practical implicationsWhile farmers now consider CAW as anex antemeasure to climate risks, a technology knowledge gap exists, which limits its adoption. Therefore, designing appropriate methods to communicate scientific evidence is crucial.Originality/valueThis paper uses three years panel data from 184 farm households in Haryana, India, together with focus groups discussions with farmers and interviews with key informants to assess if farmers learn adaptation to climate change from past climate extremes.
Examining work factors after Malaysia’s minimum wage implementation
Authors: Che Ahmat Nur Hidayah , Arendt Susan Wohlsdorf , Russell Daniel Wayne 
Published: August 2019

Purpose
This study aims to generate novel insights about minimum wage policy implementation through a joint assessment of the mediating roles of work motivation, work engagement and job satisfaction in predicting outcomes such as turnover intention and work engagement.

Design/methodology/approach
Data were collected from hotel employees in Malaysia using an electronic survey. A total of 239 responses were used in analyzing direct and indirect effects.

Findings
Results reveal that work motivation, work engagement and job satisfaction significantly mediated the relationship between employee compensation satisfaction and employee turnover intention. Work motivation was found to mediate the relationship between employee compensation satisfaction and employee work engagement. Additionally, work engagement and job satisfaction mediated the relationship between employee work motivation and employee turnover intention.

Research limitations/implications
Missing data are inevitable in survey research. Due to data missing for some of the demographic questions, the moderating effect of certain demographic characteristics (e.g. sex) could not be assessed.

Practical implications
Given recent minimum wage policy implementation in Malaysia, it is imperative that Malaysian hotel operators understand to what extent employee compensation satisfaction influences how employees perceived their jobs and to what extent work motivation, work engagement and job satisfaction mediate employee compensation satisfaction and employee turnover intention.

Originality/value
This study makes a significant contribution to the hospitality compensation research area, specifically regarding the impact of compensation on how employees perceived their jobs after minimum wage implementation.
Investigating technology and organizational performance of private higher learning institutions in Malaysia
Authors: Chan Ie Lyn Josephine , Muthuveloo Rajendran 
Published: August 2019

Purpose
The purpose of this paper is to investigate the influence of technology on organizational performance of private higher learning institutions (HLIs) in Malaysia and to determine the area of focus for private HLIs in Malaysia.

Design/methodology/approach
Data collection was carried out over two months through an online self-administered questionnaire and yielded 155 samples. Subsequently, the partial least squares structural equation modeling (PLS-SEM) was used to test the reflective measurement model and the structural model for validity, reliability and hypotheses, respectively.

Findings
This paper discovered that between the two constructs of technology tested (technology management and technology usage), only technology management influenced organizational performance.

Originality/value
As Industry 4.0 is disrupting the existing business environment, inquiring into the influence of technology is of critical importance for the organizational performance of private HLIs in Malaysia. This paper provides a different perspective of how technology affects the overall organizational performance of private HLIs which differs from past studies which focused more on the effects of technology on individuals such as teachers/instructors and learners.
Mentorship for flourishing in schools: an explicit shift toward appreciative action
Authors: Cherkowski Sabre , Walker Keith 
Published: August 2019

Purpose
Building on findings from research designed to bring to description teachers’ own understandings of what it means to flourish in their work, the purpose of this paper is to show how principals and teacher–leaders in schools are agents capable of building developmental relationships and mentoring cultures that can orient and support teachers toward well-being.

Design/methodology/approach
This conceptual paper is anchored with findings from a multi-year qualitative research project that was designed using perspectives from positive organizational studies and positive psychology scholarship. The research methods encompassed collaborative and generative use of appreciative inquiry and strength-based research activities in school districts in both British Columbia and Saskatchewan, Canada. Data used to build this conceptual paper are from appreciative focus group conversations with teachers and principals over the course of two years. Conversations were recorded and transcribed. Transcripts were thematically analyzed using the research literature framing the study.

Findings
This paper offers four domains of inquiry and a model for flourishing schools that encourages principals and teacher–leaders to develop habits of mind and heart to build developmental relationships in ways that help both mentors and mentees to embody and enact positive, appreciative and generative ways of noticing, nurturing and sustaining the work of teaching and learning; all with aspiration to sustain and enhance the well-being of every member of the school community.

Practical implications
This paper offers conceptual models and storied descriptions that can aid mentors in noticing and nurturing more developmental relationship approaches to mentoring for well-being as opportunities to build mentoring relationships from appreciative and growth-based habits and approaches. As these relationships are built across the school, positive mentoring cultures may foster and grow in ways that promotes a flourishing-for-all approach to teaching and learning.

Originality/value
This paper contributes a different and complimentary perspective to research and practice on mentoring, offering a positive organizational perspective that highlights and promotes the perceived and evidenced benefits of deliberately focusing on what goes well and what provokes vitality in schools. The conceptual models in this paper offer tools for mentors and mentees to develop and foster in others appreciative and positive capacities for positive mentoring.
Wine place research
Authors: Warman Russell D. , Lewis Gemma K. 
Published: August 2019

Purpose
Place is an important aspect of wine quality, contributing both distinct sensory characteristics and symbolic significance to the consumer’s experience, particularly in premium wine markets. The concepts of provenance and terroir, and the processes they describe, communicate meaning and significance along the value chain from wine production to consumption. This paper aims to clarify how these concepts are defined, how they contribute to premium wine value chains and how a greater understanding of these concepts by wine science researchers, and other actors, can enhance consumer value.

Design/methodology/approach
To address these aims, a conceptual framework is developed, which outlines the conditions needed to fulfil the wine/place experience through the value chain. This framework resulted from discussions within a team of researchers currently undertaking a large project into place distinctiveness in Pinot Noir wines in Australia. The refinement and exploration of the concept is grounded in a multidisciplinary literature review.

Findings
Through application of the framework, wine science researchers are advised to develop a knowledge co-production approach with other actors in the value chain. Doing so enables all actors to use evidence-based storytelling to enhance the role that place has in premium wine value and consumer experience.

Originality/value
Overall, this paper contributes to the conversation surrounding the value of terroir and provenance, particularly as they relate to premium wine in New World wine regions. The innovative framework is applicable for both business and wine science researchers, especially those with decision-making responsibility and associated with wine science research institutions, funding bodies, industry partnerships and consortia.