bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osteocalcin does not influence acute or chronic inflammation in human vascular cells
Authors: Millar Sophie A. , Zala Ieva , Anderson Susan I. , O'Sullivan Saoirse E. 
Published: September 2019

AbstractSome human observational studies have suggested an anti‐inflammatory role of osteocalcin (OCN). An inflammatory protocol using interferon‐γ and tumor necrosis factor‐α (10 ng/ml) was employed to examine the acute (24 hr) and chronic (144 hr) effects of uncarboxylated OCN (ucOCN) in commercial, primary, subcultured human aortic endothelial cells (HAEC), and human smooth muscle cells (HASMCs). The inflammatory protocol increased phosphorylation of intracellular signaling proteins (CREB, JNK, p38, ERK, AKT, STAT3, STAT5) and increased secretion of adhesion markers (vascular cell adhesion molecule‐1, intracellular adhesion molecule‐1, monocyte chemoattractant protein‐1) and proinflammatory cytokines (interleukin‐6 [IL‐6], IL‐8). After acute inflammation, there were no additive or reductive effects of ucOCN in either cell type. Following chronic inflammation, ucOCN did not affect cell responses, nor did it appear to have any pro‐ or anti‐inflammatory effects when administered acutely or chronically on its own in either cell type. Additionally, ucOCN did not affect lipopolysaccharide (LPS)‐induced acute inflammation in HAECs or HASMCs. The findings of this study do not support a causal role for OCN within the models of vascular inflammation chosen. Further confirmatory studies are warranted.
SENP1‐mediated NEMO de‐SUMOylation inhibits intermittent hypoxia induced inflammatory response of microglia in vitro
Authors: Yang Tianyun , Sun Jinyuan , Wei Bo , Liu Song 
Published: September 2019

AbstractAmong the seven small ubiquitin‐like modifier (SUMO)‐specific proteases (SENPs), our previous work showed that SENP1 suppressed nuclear factor kappa B (NF‐κB) activation and alleviates the inflammatory response in microglia. However, the mechanism is still largely unknown. In this study, western blot analysis and enzyme‐linked immunosorbent assay were utilized for evaluating the extent of NF‐κB activation and expression of proinflammatory cytokines. qPCR and western blot analysis were performed to detect SENP1 expression. Coimmunoprecipitation followed by western blot analysis was applied to measure the changes in SUMOylation of NF‐κB essential modulator (NEMO) and P65 in microglia with or without overexpression of SENP1. As the results, we found that intermittent hypoxia (IH) triggered the activation of NF‐κB and upregulated the expression levels of tumor necrosis factor‐α and interleukin‐6. Interestingly, our data indicated that the SUMOylation of NEMO was enhanced by IH while SUMOylation of P65 was not affected. Further, our data showed that overexpression of SENP1 could decrease the extent of NF‐κB activation and inhibit the inflammatory response of microglia through regulating the SUMOylation of NEMO. Collectively, this study presents the first report of the SENP1‐controlled de‐SUMOylation process of NEMO and its critical role in regulating NF‐κB activation and proinflammatory cytokines secretion in microglia cells. This study would benefit for clarifying the role of SENP1 in IH‐induced activation of microglia, thus providing potential therapeutic targets for obstructive sleep apnea treatment.
The interplay between aging‐associated loss of protein homeostasis and extracellular vesicles in neurodegeneration
Authors: Guix Francesc X. 
Published: September 2019

AbstractThe finding of an effective cure or treatment for neurodegenerative diseases is one of the biggest challenges for this century. Although these diseases show different clinical manifestations, the presence of toxic protein aggregates in the brain of patients is a common feature to all of them, suggesting a loss of protein homeostasis. Aging, the primary risk factor for the majority of neurodegenerative disorders, is linked to the impairment of degradative compartments such as lysosomes and autophagosomes. Besides, many genetic factors for Alzheimer's disease, Parkinson's disease, or frontotemporal dementia, as examples of frequent neurodegenerative diseases, are causative of endo‐lysosomal and autophagosomal dysfunctions. There is scientific evidence suggesting that neurons can counteract the accumulation of undegraded cellular material by the secretion of extracellular vesicles (EVs), which are vesicles with a size ranging from 50 to 100 nm generated in a type of endosomal compartment named multivesicular body. EVs play a crucial role in removing cellular waste, promoting protein aggregation, and spreading toxic protein aggregates in the brain of patients. In this review, the interplay between the impairment of degradative compartments, the secretion of EVs, and their pathological/beneficial role in neurodegeneration is described.
Creation of a Standard Model for Tube Feeding at Neonatal Intensive Care Unit Discharge
Authors: White Benjamin R. , Ermarth Anna , Thomas Debbie , Arguinchona Olivia , Presson Angela P. , Ling Con Yee 
Published: September 2019

AbstractBackgroundFeeding dysfunction is a common consequence of prematurity and illness in neonates, often requiring supplemental nasogastric (NG) or gastrostomy (GT) feeding tubes. A standardized approach to the discharge of infants receiving home enteral nutrition (HEN) is currently lacking.MethodsThe Home Enteral Feeding Transitions (HEFT) program was developed to identify patients eligible for HEN and create a standard discharge process. A structured tool helped determine discharge timing and route, and a dedicated outpatient clinic was created for infants discharged on HEN. Demographic, inpatient, and outpatient data were prospectively collected and compared with a historical cohort.ResultsA total of 232 infants discharged from our neonatal intensive care unit (NICU) over 9 months met inclusion criteria. Ninety‐eight (42%) were discharged with HEN, 68 NG and 30 GT, compared with 134 (58%) receiving full oral feeds. This represented a 10% increase in HEN utilization (P = 0.003) compared with our historical control group. Median HEN length of stay was 31.5 days compared with our historical average of 41 days (P = 0.23). Frequency of emergency department visits and admissions because of HEN was unchanged postintervention. Parents were satisfied (8.6/10), and 98% said they would choose HEN again. The median time to NG discontinuation after discharge was 13.5 days, with an estimated cost savings of $2163 per NICU day.ConclusionOur program is the first of which we know to use a standard care‐process model to guide the decision‐making and utilization of HEN at NICU discharge. HEFT shows that HEN at NICU discharge can be safe and effective, with high parental satisfaction.
Does race impact survival for patients with anal squamous cell carcinoma?
Authors: Fields Adam C. , Welten Vanessa M. , Lu Pamela , Goldberg Joel E. , Irani Jennifer , Bleday Ronald , Melnitchouk Nelya 
Published: September 2019

AbstractBackground/ObjectivesRacial disparities are known to impact cancer outcomes. The aim of this study was to assess current racial disparities in outcomes of anal squamous cell carcinoma (SCC).MethodsThe National Cancer Database was used to identify patients with anal SCC. The primary outcome was 5‐year overall survival.ResultsThere were 32 255 (88.1%) White patients and 4342 (11.9%) Black patients identified with anal SCC. Compared to White patients, Black patients were more likely to be younger, have lower median income, and be insured with Medicaid (all P < .001). The 5‐year overall survival of Black and White patients for stage I disease was 71.2% and 80.6% (P < .001), for stage II disease, was 64.6% and 69.3% (P = .001), for stage III disease was 50.9% and 58.1% (P < .001), and for stage IV disease was 22.1% and 21.9% (P = .20). In a cox regression analysis, Black race was associated with significantly worse survival in stage I (HR: 1.37, 95% CI: 1.07‐1.76, P = .01), stage II (HR: 1.30, 95% CI: 1.14‐1.48, P < .001), and stage III disease (HR: 1.31, 95% CI: 1.16–1.47, P < .001) but not for stage IV disease (HR: 1.09, 95% CI: 0.89‐1.35, P = .41).ConclusionsBlack race is correlated with worse survival in patients diagnosed with anal SCC. This disparity in survival is likely multifactorial and requires further study.
Nanogram/mL detection of nortriptyline: Preparation of a molecularly imprinted polymer for spectrophotometric determination of nortriptyline based on multivariate optimization methods
Authors: Nezhadali Azizollah , Mehrdadian Zahra , Mojarrab Maliheh 
Published: September 2019

AbstractIn this work, the molecularly imprinted polymer was used as a selective sorbent in solid‐phase extraction method for the spectrophotometric determination of nortriptyline at 239 nm. Molecularly imprinted polymer was synthesized by pyrrole as a functional monomer in the presence of nortriptyline as a template. Several factors, consist of the concentration of the monomer to template ratio, amount of initiator, stirring rate, reaction time, the pH of the buffer solution, amount of sorbent, loading time, shaking rate of loading, extraction time, and shaking rate of extraction were evaluated due to their effectiveness in the preparation and extraction capability of molecularly imprinted polymer. Multivariate optimization methods, such as Plackett‐Burman and central composite designs, were employed to find and optimize the significant factors. Under the selected optimal conditions, molecularly imprinted polymer showed a linear range from 0.1 to 100 µmol/L (0.026 to 26 µg/mL) nortriptyline, a detection limit of 10.3 nmol/L (2.7 ng/mL), a highly repeatable (relative standard deviation of 3.7%) and reproducible response (relative standard deviation of 4.6%), and a good selectivity in the presence of structurally related molecules. Furthermore, molecularly imprinted polymer showed high extraction efficiency and was successfully used for the determination of nortriptyline in real samples.
Label‐ and modification‐free‐based in situ selection of bovine serum albumin specific aptamer
Authors: Wang Chuchu , Du Xiaoyan , Xie Tiantian , Li Hao 
Published: September 2019

AbstractSystematic evolution of ligands by exponential enrichment is a traditional approach to select aptamer, which has a great potential in biosensing field. However, chemical modifications of DNA library or targets before selection might block the real recognition and binding sites between aptamers and their targets. In this study, a label‐ and modification‐free‐based in situ selection strategy was developed to overcome this limitation. The strategy is an attempt to screen bovine serum albumin aptamers according to the principle of electrophoretic mobility shift assay, and allowed single‐stranded DNA sequence to be fully exposed to interact with bovine serum albumin which was mixed with the agarose gel beforehand. After eight rounds of selection, specific aptamer with low dissociation constant (Kd) value of 69.44 ± 7.60 nM was selected and used for subsequent establishment of fluorescence biosensor. After optimization, the optimal aptasensor exhibited a high sensitivity toward bovine serum albumin with a limit of detection of 0.24 ng/mL (linear range from 1 to 120 ng/mL). These results indicated that the label‐ and modification‐free‐based in situ selection strategy proposed in this work could effectively select specific aptamer to develop aptasensor for sensitive detection of bovine serum albumin or other targets in actual complicated samples.
Verification of cheeses authenticity by mass spectrometry
Authors: Kuckova Stepanka , Zitkova Kristyna , Novotny Ondrej , Smirnova Tatiana 
Published: September 2019

AbstractCheeses are a group of fermented dairy products that are produced all over the world in various forms and flavours. Milk, especially sheep or goat milk, is still regarded as an expensive raw material in the world, which makes milk and milk products highly attractive as a fraud target. Most often, such fraud includes partial or complete substitution with cheaper sorts of milk (e.g. bovine milk). The aim of this work was to verify the authenticity of 27 cheeses commonly emerging on the Czech food market. The cheeses were distinguished on the basis of milk animal species origin. For this purpose, two mass spectrometry techniques were used: matrix‐assisted laser desorption/ionization with time of flight mass spectrometry together with principal component analysis method and liquid chromatography coupled with electrospray ionization and quadrupole time‐of‐flight mass spectrometry. The results were a partial success, because the cheeses could only be partially distinguished with the first mass spectrometry technique probably because of the influence of some protein additive materials in cheeses. The second technique allowed for collecting higher quality results and thus appears to be highly suitable for the research task.
Polyionic Liquids (PIL) Promoted Ce Doped ZnO for the Photocatalytic Degradation of Rhodamine B (RhB)
Authors: Li Rong‐rong , Cao Jia‐jie , Huang Yang‐ru , Yao Yu‐feng , Zhang Ze‐kai , Fan Hua‐Jun , Zhao Jia , Han De‐man 
Published: September 2019

AbstractIn this study, an efficient one‐pot method was developed to synthesize Ce‐doped ZnO photocatalysts in the polyionic liquids. The synthesized samples have better photocatalytic degradation activity than pure ZnO. The excellent catalytic performance may be due to the fact that the doping of Ce increased the surface dispersion of the ZnO nanoparticles, and the addition of the polyionic liquids further enhanced the dispersion.And the addition of polyionic liquids increased the dispersion of ZnO particles and doped Ce centers, which greatly limits the photogenerated electron‐hole recombination, thereby increasing the photocatalytic degradation activity of the material.
A High Stretchable and Self–Healing Silicone Rubber with Double Reversible Bonds
Authors: Yan Hao , Dai Shengping , Chen Yuewen , Ding Jianning , Yuan Ningyi 
Published: September 2019

AbstractIt is a challenge to synthesize silicone rubber with high stretchability and rapid self–healing ability without external stimuli. This article reports on a new class of self–healing poly(dimethylsiloxane)(PDMS) elastomer fabricated by introducing double reversible bonds (imine and hydrogen bonds) into silicone chains. Through reasonable structural design, the silicone rubber with high‐efficiency self‐healing ability was produced. The as–prepared PDMS elastomer films exhibited high stretchability (>4000%), good transparency (>80%) and fast self–healing efficiency (3 h at room temperature a healing efficiency of 93%). It could also be healed at low temperature (‐20 °C) and processed cyclically. In addition, we combined carbon nanotube films with this elastomer to prepare a flexible and stretchable capacitive sensor through a simple process.
Self‐Assembled Uniform Silver Nanoparticles (SAAgNPs) and Their Supported MoO3 Nanocatalysts for Effective Degradation of Azo Dyes
Authors: Rajesh Rajendiran , Arunkumar Patchaiyappan , Putrakumar Balla , Venkatesan Rengarajan 
Published: September 2019

AbstractIn the present study, we report a novel self assembled uniform 3.9 nm silver nanoparticles (SAAgNPs), and their supported MoO3 (Ag/MoO3) nanocatalysts prepared by a simpler chemical method. The excellent metal/metal oxide interaction between Ag and MoO3 has been exploited for catalytic degradation of azo dyes. The significant enhancement of azo dye degradation in the catalytic experiments was examined using UV‐Vis absorption kinetics. The 4 wt % SAAgNPs loaded MoO3 heterogeneous nanocatalyst (Ag/MoO3) showed the highest azo dye degradation efficiency i. e., 37.9 x 10−3 s−1, 19.1 x 10−3 s−1 for methyl orange (MO) and congo red (CR) respectively compared to that of a bare SAAgNPs in presence of NaBH4. The morphology, detailed size, electronic nature of the synthesized catalysts (SAAgNPs and Ag/MoO3) has been investigated using TEM, HRTEM, XRD, UV‐Vis and XPS studies.
The influence of wind in secondary settling tanks for wastewater treatment – A computational fluid dynamics study. Part II: Rectangular secondary settling tanks
Authors: Gao Haiwen , Stenstrom Michael K. 
Published: September 2019

AbstractComputational fluid dynamics (CFD) model is used to study the effect of wind on the performance of a rectangular secondary sedimentation tank (SST) in a wastewater treatment plant (WWTP). Unlike most of the previous CFD modeling studies which evaluated the wind effect on the sedimentation tank in only water treatment plants, this study evaluates a rectangular SST in a WWTP at different wind speeds and directions, and under different inflow loading conditions. The wind is qualitatively and quantitatively analyzed for a range of wind speeds and directions as well as loading rates. The net effect is to change the three‐dimensional hydrodynamics profiles, effluent suspended solids, and sludge blanket height. The simulation results show that the wind deteriorates overall clarification performance of the rectangular SST and has little effect on the sludge thickening under mild wind conditions until the speed increases an extreme windy condition. These CFD simulation results suggest that in strong windy climates, covering SSTs or protecting them from strong winds may be justified.Practitioner points

This is the first comparison of wind effects on a rectangular secondary sedimentation tank

The effects of varied flow conditions, wind directions, and velocities are compared

Wind‐induced currents in the settling tank negatively affect removal efficiency

Winds have strong negative impact on the performance of sedimentation tanks
Depth of Invasion into the Circular and Longitudinal Muscle Layers in T2 Esophageal Squamous Cell Carcinoma Does Not Affect Prognosis or Lymph Node Metastasis: A Multicenter Retrospective Study
Authors: Tian Dong , Huang Heng , Yang Yu‐Shang , Jiang Kai‐Yuan , He Xi , Guo Xiao‐Guang , Chen Long‐Qi 
Published: September 2019

AbstractBackgroundAlthough a greater depth of tumor invasion is correlated with a poorer prognosis in esophageal squamous cell carcinoma (ESCC), it remains controversial whether T2 ESCC should be subclassified by circular and longitudinal muscle invasion. We conducted a multicenter retrospective study to evaluate the relationship between the depth of invasion and long‐term outcome and to identify the clinical significance of subclassifying T2 ESCC.MethodsPatients with T2 ESCC who underwent esophagectomy at two different institutes between January 2009 and December 2017 were analyzed retrospectively. ESCC with circular and longitudinal muscle invasion was defined as T2 circular and T2 longitudinal ESCC, respectively. Survival outcomes and risk factors for lymph node metastasis (LNM) were evaluated by univariate and multivariate analyses. In addition, data from stage T1b ESCC cases during the same period were retrieved for use as a comparison cohort to evaluate the prognostic significance of the T2 substage.ResultsA total of 536 T2 ESCC patients were eligible, and 192 (36%) patients developed LNM. No significant difference was found in general characteristics between the T2 circular and T2 longitudinal ESCC groups (n = 219 and n = 317, P > 0.05), except for tumor location (P = 0.02). The T2 substage was not significantly correlated with survival on univariate or multivariate analysis (P = 0.30 and P = 0.34, respectively). Multivariate analysis also indicated that the T2 substage was not an independent risk factor for LNM (P = 0.15). When patients with stage T1b ESCC were considered, their survival time was significantly different from that of patients with T2 circular and T2 longitudinal disease (P = 0.01).ConclusionsThe depth of tumor invasion into the circular and longitudinal muscle layers in T2 ESCC does not affect the prognosis or risk of LNM.
Climate sensitivity and resistance under pure- and mixed-stand scenarios in Lower Austria evaluated with distributed lag models and penalized regression splines for tree-ring time series
Authors: Nothdurft Arne , Engel Markus 
Published: September 2019

Abstract
Penalized regression splines and distributed lag models were used to evaluate the effects of species mixing on productivity and climate-related resistance via tree-ring width measurements from sample cores. Data were collected in Lower Austria from sample plots arranged in a triplet design. Triplets were established for sessile oak [Quercus petraea (Matt.) Liebl.] and Scots pine (Pinus sylvestris L.), European beech (Fagus sylvatica L.) and Norway spruce [Picea abies (L.) H. Karst.], and European beech and European larch (Larix decidua Mill.). Mixing shortened the temporal range of time-lagged climate effects for beech, spruce, and larch, but only slightly changed the effects for oak and pine. Beech and spruce as well as beech and larch exhibited contrasting climate responses, which were consequently reversed by mixing. Single-tree productivity was reduced by between − 15% and − 28% in both the mixed oak–pine and beech–spruce stands but only slightly reduced in the mixed beech–larch stands. Measures of climate sensitivity and resistance were derived by model predictions of conditional expectations for simulated climate sequences. The relative climate sensitivity was, respectively, reduced by between − 16 and − 39 percentage points in both the beech–spruce and beech–larch mixed stands. The relative climate sensitivity of pine increased through mixing, but remained unaffected for oak. Mixing increased the resistance in both the beech–larch and the beech–spruce mixed stand. In the mixed oak–pine stand, resistance of pine was decreased and remained unchanged for oak.
“Displaced archives”: proposing a research agenda
Authors: Lowry James 
Published: September 2019

AbstractIn the opening keynote speech at the Eighth International Conference on the History of Records and Archives (I-CHORA 8) in Melbourne, Australia, the author provided an overview of archival displacement as an historical phenomenon, before concentrating on postcolonial cases and arguing for a fuller global history of the displacement of archives during decolonisation. The talk concluded with some thoughts on future directions for research on displaced archives. Understanding the term “displaced archives” to refer to any records that have been removed from the context of their creation and whose ownership is disputed, this short article elaborates further upon a potential research agenda for displaced archives, which remains an under-researched area in archival studies.
Canada in the United Nations General Assembly from Trudeau to Trudeau
Authors: Vucetic Srdjan , Ramadanovic Bojan 
Published: September 2019

AbstractAll Canadian governments say that Canada must look to its friends and allies and like-minded partners to achieve greater cooperation on global issues. But who are these countries exactly? To gain a better understanding of where Ottawa stands in the world, with whom and under what conditions, we analyze Canada's voting patterns in the United Nations General Assembly from 1980 to 2017. We find that Canada's overall record tends toward that of Western European states. We find no evidence of greater affinity with US positions either when the Democrats are in power in Washington or when the conservative parties are in power in Ottawa. We identify a sharp pro-US turn in the Harper years and also confirm that the government of Justin Trudeau started off by maintaining rather than reversing this trend.