bluuebunny/crossref_metadata_2025_split huggingface dataset:
Necrostatin-1 accelerates time to death in a rat model of cecal ligation and puncture and massively increases hepatocyte caspase-3 cleavage
Authors: Zhang Qin , Wei Siwei , Lu Jiayin , Fu Weijun , Chen Hui , Huang Qiaobing , Chen Zhongqing , Zeng Zhenhua 
Published: February 2019

Necroptosis, a form of regulated necrosis, has been reported to be involved in numerous pathologies, including sepsis. However, a protective effect of the selective inhibitor of necroptosis, necrostatin-1 (Nec-1), against sepsis remains to be confirmed. Animals (rats and mice) were subjected to cecal ligation and puncture (CLP) to mimic clinical sepsis. Nec-1 or its vehicle (control) was administered 20 min before CLP. Survival time was observed up to 72 h after CLP. Specimens of liver tissue and serum were obtained at 6 h, 12 h, and 18 h. Expression of necroptosis-related proteins [receptor-interacting protein kinase (RIP)1, RIP3, and mixed lineage kinase domain-like (MLKL)] was determined by Western blot analysis. The RIP1/RIP3 interaction and the recruitment of MLKL to RIP3 were also analyzed. Liver function, histopathological changes, serum inflammation cytokines, TUNEL staining, and the expression of apoptosis-related protein, including caspase-3, B-cell lymphoma 2 (Bcl-2), and Bcl-2-associated X (Bax), was determined. As expected, Nec-1 administration reduced the expression of necroptosis-related proteins and the RIP1/RIP3 interaction, indicating inhibited necroptosis. Surprisingly, Nec-1 treatment exacerbated the liver injury and shortened survival time of septic rats with increased TUNEL-positive cells, cleaved caspase-3 protein content, and Bax/Bcl-2 ratio. Collectively, these findings show that Nec-1 administration inhibited the hepatocyte necroptosis pathway but accelerated apoptosis via the apoptotic pathway in CLP-induced sepsis rat. NEW & NOTEWORTHY The present study demonstrated that a chemical inhibitor necrostatin-1 (Nec-1) or receptor-interacting protein kinase(RIP1) knock down targeted at necroptosis inhibition accelerated liver injury of following sepsis. For fundamental research, these results warrant further investigation of the potential link between Nec-1 administration and the cellular apoptosis following sepsis induced liver injury. For applied research, these results suggest the potential harmful effect of Nec-1 on future sepsis treatment.
Left ventricular vortex and intraventricular pressure difference in dogs under various loading conditions
Authors: Matsuura Katsuhiro , Shiraishi Kenjirou , Sato Kotomi , Shimada Kazumi , Goya Seijirow , Uemura Akiko , Ifuku Mayumi , Iso Takeshi , Takahashi Ken , Tanaka Ryou 
Published: February 2019

Restrictions on the conventional evaluation of diastolic function have been recognized, especially under various loading conditions. Recently, new noninvasive ventricular vortex indexes have been introduced and are expected to reflect the cardiac function. Physiologically, there is a hypothesis that the intraventricular pressure difference (IVPD) is related to the formation of vortexes. IVPD and vortex indexes were simultaneously measured, and the relationship between the two was investigated. To verify the possibility of diastolic vorticity as an index of diastolic relaxation, a correlation between diastolic vorticity and the load dependency of vorticity [time constant (τ)] was examined. Six healthy dogs were studied using transthoracic echocardiography, pressure, and a conductance catheter. Vorticity was analyzed using vector flow mapping (VFM). IVPD was determined using Euler’s equation with color M-mode Doppler images. Data were obtained at baseline, at balloon dilatation in the thoracic aorta to alter afterload, at hydroxyethyl starch infusion to alter preload, and at milrinone administration to alter ventricular relaxation. Peak vorticity at early diastole (E-Vor) and IVPD of the midventricle (MIVPD) decreased under pressure loading, were unchanged under volume loading, and increased during milrinone administration. In multivariate analysis, the independent predictors of τ were global longitudinal strain, strain rate at early diastole, and E-Vor. MIVPD was strongly correlated with E-Vor ( r = 0.84). VFM-derived peak E vorticity was strongly related to IVPD, especially MIVPD, under various loading conditions. Both of these novel indexes are promising as reliable indexes of ventricular relaxation, independent from preload. NEW & NOTEWORTHY We showed the close relationship of vortex and intraventricular pressure difference and showed that both of them can become new markers of the left ventricular relaxation property. Our present study creates a paradigm for future studies in the field of intraventircular flow physiology and clinical diastology.
VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER
Authors: YULNAWATI YULNAWATI , H. MAHESHWARI  , HERDIS  , M. RIZAL  
Published: February 2019

The objective of this study was to obtain the viability and plasma membrane integrity of the spotted buffalo epididymal sperm after addition of dextrose into AndromedÃ’ extender.Â Spermatozoa that have been collected from cauda epididymis were diluted with AndromedÃ’ extender as control (K) and AndromedÃ’ + 0.2% dextrose (P1) and AndromedÃ’ + 0.4% dextrose (P2) as treatments.Â The results showed that the quality of epididymal spermatozoa decreased during cryopreservation process.Â The percentage of motility after thawing in P1 (46%) and P2 (46.67%) were significantly higher (P
INDUCTION OF CALLUS FORMATION FROM DIFFERENT PARTS OF Citrus grandis (OSBECK) FLOWERS
Authors: Zakaria Zarina Binti , Zakaria Suhana Binti , Khalid Abd Hamid Bin , Mohd Ishak Mohd Azlan Bin 
Published: February 2019

Â Â Â Â Â Â Â Â Â Â Â The objectives of the present work were to study the capability of pomeloâ€™s floral tissues to produce callus and to investigate the influence of plant growth regulators on callus induction and development. Various parts of flower namely petal, sepal, style, ovary, pistil and cup base were cultured onto Murashige and Skoog (MS) basal medium supplemented with different level of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and 6-Furfurylaminopurine (kinetin). It was found that different part of flowers favored different level of hormone for callus induction. The highest formation of callus were obtained from petal and sepal cultured on MS media supplemented with 1.5 mg/l kinetin. For the style and pistil, 50 Â± 2.3% of the explants had developed callus when cultured on MS media supplemented with 0.05 mg/l and 0.10 mg/l BAP respectively. On the other hand, sucrose agar (SA) media alone had managed to induce callus formation from almost every part of the flowers with a success rate between 7.69 Â± 0.32 to 50 Â± 3.18%. Callus formation was preferred from the uncut part of flowers as shown in the study on petal with percentage of formation was 14.6 Â± 0.35%.
ESTIMATION OF SEAGRASS COVERAGE BY DEPTH INVARIANT INDICES ON QUICKBIRD IMAGERY
Authors: Amran Muhammad Anshar 
Published: February 2019

Management of seagrass ecosystem requires availability of information on the actual condition of seagrass coverage. Remote sensing technology for seagrass mapping has been used to detect the presence of seagrass coverage, Â but so far no information on the condition of seagrass could be obtained. Therefore, a research is required using remote sensing imagery to obtain information on the condition of seagrass coverage. The aim of this research is to formulate mathematical relationship between seagrass coverage and depth invariant indices on Quickbird imagery. Transformation was done on multispectral bands which could detect sea floor objects that are in the region of blue, green and red bands. The study areas covered are the seas around Barranglompo Island and Barrangcaddi Island, westward of Makassar city, Indonesia. Various seagrass coverages were detected within the region under study. Mathematical relationship between seagrass coverage and depth invariant indices was obtained by multiple linear regression method. Percentage of seagrass coverage (C) was obtained by transformation of depth invariant indices (Xij) on Quickbird imagery, with transformation equation as follows: C = 19.934 â€“ 63.347 X12 + 23.239 X23. A good accuracy of 75% for the seagrass coverage was obtained by transformation of depth invariant indices (Xij) on Quickbird imagery. Â Keywords: seagrass coverage, depth invariant index
Utility of matK Gene as DNA Barcode to Assess Evolutionary Relationship of Important Tropical Forest Tree genus Mangifera (Anacardiaceae) in Indonesia and Thailand
Authors: Hidayat Topik - , Pancoro Adi - , Kusumawaty Diah - 
Published: February 2019

MaturaseK (matK) gene of chloroplast DNA has been served as appropriate candidate to be a DNA barcode in angiosperms. Using this DNA marker, 19 species of genus Mangifera, one of ecologically important crop, collected from Indonesia and Thailand were analyzed. Phylogenetic analysis using parsimony method revealed that the gene could clasify Mangifera into three major groups, namely group I, II, and III. Moreover, the matK barcode can identify Mangifera species that is originated from Thailand. Although this classification system is different with the previous system, it can provide a new information about Mangifera taxonomy. Results further exhibited that DNA sequences of the matK of two Mangifera species (M. laurina dan M. macrocarpa) are different between Indonesia and Thailand specimens.Â Keywordsâ€” DNA barcode, Mangifera, matK gene, parsimony, phylogenetic analysis
Thrombus Length Predicts Lack of Post-Thrombolysis Early Recanalization in Minor Stroke With Large Vessel Occlusion
Authors: Seners Pierre , Delepierre Julie , Turc Guillaume , Henon Hilde , Piotin Michel , Arquizan Caroline , Cho Tae-Hee , Lapergue Bertrand , Cottier Jean-Philippe , Richard Sébastien , Legrand Laurence , Bricout Nicolas , Mazighi Mikaël , Dargazanli Cyril , Nighoghossian Norbert , Consoli Arturo , Debiais Séverine , Bracard Serge , Naggara Olivier , Leclerc Xavier , Obadia Michael , Costalat Vincent , Berthezène Yves , Tisserand Marie , Narata Ana-Paula , Gory Benjamin , Mas Jean-Louis , Oppenheim Catherine , Baron Jean-Claude ,   on behalf of the PREDICT-RECANAL Collaborators
Published: February 2019

Background and Purpose—
Whether bridging therapy, that is, intravenous thrombolysis [IVT] followed by mechanical thrombectomy, is beneficial as compared with IVT alone in minor stroke (National Institutes of Health Stroke Scale ≤5) with large vessel occlusion is unknown and should be tested in randomized trials. To help select the most appropriate candidates for such trials, we aimed to identify strong predictors of lack of post-IVT early recanalization (ER)—a surrogate marker of poor outcome.

Methods—
From a large multicenter French registry of patients with large vessel occlusion referred for thrombectomy immediately after IVT start between 2015 and 2017, we extracted 97 minor strokes with ER evaluated on first angiographic run or noninvasive imaging ≤3 hours from IVT start. Thrombus length was measured using the susceptibility vessel sign on T2* imaging.

Results—

Median National Institutes of Health Stroke Scale was 3 (interquartile range, 2–4), and occlusion sites were proximal (intracranial carotid or M1) and distal (M2) in 50% and 50% of patients, respectively. On pre-IVT MRI, median length of susceptibility vessel sign (visible in 90%) was 9.2 mm (interquartile range, 7.4–13.3). ER was present in 34% of patients, and susceptibility vessel sign length was the only clinical or radiological variable associated with no-ER after stepwise variable selection into a multivariable model (odds ratio, 1.53 per 1-mm increase; 95% CI, 1.21–1.92;
P
<0.001). The C statistic of susceptibility vessel sign length for no-ER prediction was 0.82 (95% CI, 0.73–0.92), and the optimal cutoff (Youden) was 9 mm. Sensitivity and specificity of this cutoff for no-ER were 67.8% (95% CI, 55.9–79.7) and 84.6% (95% CI, 70.7–98.5), respectively.

Conclusions—
ER was frequent in this cohort of IVT-treated minor stroke patients with large vessel occlusion considered for thrombectomy, and thrombus length was a powerful independent predictor of no-ER. These findings may help design randomized trials aiming to test bridging therapy versus IVT alone in this population.
Cigarette Smoking History and Functional Outcomes After Spontaneous Intracerebral Hemorrhage
Authors: Chen Ching-Jen , Ding Dale , Ironside Natasha , Buell Thomas J. , Southerland Andrew M. , Koch Sebastian , Flaherty Matthew , Woo Daniel , Worrall Bradford B. ,   on behalf of the ERICH Investigators
Published: February 2019

Background and Purpose—
Although cigarette use may be a risk for intracerebral hemorrhage (ICH), animal models suggest that nicotine has a potential neuroprotective effect. The aim of this multicenter study is to determine the effect of smoking history on outcome in ICH patients.

Methods—
We analyzed prospectively collected data from the Ethnic/Racial Variations of Intracerebral Hemorrhage study and included patients with smoking status data in the analysis. Patients were dichotomized into nonsmokers versus ever-smokers, and the latter group was further categorized as former (>30 days before ICH) or current (≤30 days before ICH) smokers. The primary outcome was 90-day modified Rankin Scale score shift analysis. Secondary outcomes were in-hospital mortality and mortality, Barthel Index, and self-reported health status measures at 90 days.

Results—

The overall study cohort comprised 1509 nonsmokers and 1423 ever-smokers (841 former, 577 current, 5 unknown). No difference in primary outcome was observed between nonsmokers versus ever-smokers (adjusted odds ratio [aOR], 1.041; 95% CI, 0.904–1.199;
P
=0.577). No differences in primary outcome were observed between former (aOR, 0.932; 95% CI, 0.791–1.178;
P
=0.399) or current smokers (aOR, 1.178; 95% CI, 0.970–1.431;
P
=0.098) versus nonsmokers. Subgroup analyses by race/ethnicity demonstrated no differences in primary outcome when former and current smokers were compared with nonsmokers. Former, but not current, smokers had a lower in-hospital mortality rate (aOR, 0.695; 95% CI, 0.500–0.968;
P
=0.031), which was only observed in Hispanics (aOR, 0.533; 95% CI, 0.309–0.921;
P
=0.024). Differences in self-reported health status measures were only observed in whites.

Conclusions—
Cigarette smoking history does not seem to provide a beneficial effect on 90-day functional outcome in patients with ICH.
Resource use and defence by ruby-throated hummingbirds during stopover
Authors: Zenzal Theodore J. , Moore Frank R. 
Published: February 2019

AbstractMany migrants must forageen routewhen fuel reserves are depleted and access to resources can be influenced by a variety of factors, including the sex, age, and current fuel stores of individuals, as well as resource availability. The objective of this study is to determine the factors that influence the use and defence of resources by migrating ruby-throated hummingbirds (Archilochus colubris). Our results illustrate that resource use: (1) is dependent on sex and resource defence, (2) decreases with fuel load and (3) increases with stopover duration as well as the number of unique feeders visited. Resource defence is closely tied to (1) competition, with the greatest conspecific density resulting in focal individuals being chased from feeders and (2) the interplay between resource quality and fuel load. Our research enhances our understanding of strategies migratory birds use to acquire and defend resources during stopover.
Two new fish species of the genus Puntius Hamilton (Cyprinidae) from Manipur, India, with notes on P. ticto (Hamilton) and P. stoliczkanus (Day)
Authors: LINTHOINGAMBI I. , VISHWANATH W. 
Published: February 2019

Puntius ater and P. khugae, two new species of fishes, each having a black longitudinal stripe on the side, are described from the Chindwin basin in Manipur, India. Puntius ater, which inhabits sluggish streams is distinct in having the dorsal fin edge black, the lateral line incomplete with 5–11 pored scales, 25–29 scales in the lateral row; transverse scales ½4/1/4½; preanal scales 20; and a black blotch extending over the 19th and 20th scales of the lateral-line row at the level above the posterior end of the anal fin base. Puntius khugae, inhabiting comparatively faster, clear-water streams is distinct in having the dorsal fin edge plain, the lateral line incomplete with 8–11 pored scales, 28–30 scales in the lateral row; transverse scales ½5/1/4½; preanal scales 19; and a black blotch on the 21st scale of the lateral-line row at the level above one scale behind the origin of the last anal fin ray. Morphological traits observed in P. ticto and P. stoliczkanus are given, and the status of these species is discussed
A new species of Talitridae (Amphipoda: Gammaridea) from Tioman Island, Malaysia
Authors: OTHMAN B. H.R. , AZMAN B. A.R. 
Published: February 2019

The amphipod crustacean Talorchestia morinoi sp. nov. collected on the west coast of Tioman Island, Malaysia is described and illustrated. So far no species belonging to the genus is known from Malaysia and this is the first record of the family Talitridae from Malaysia. Differences between the new species and related species within the Talorchestia sensu Morino & Miyamoto, 1988 group are discussed and a key is also included.
Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding)
Authors: KNEBELSBERGER THOMAS , MILLER MICHAEL A. 
Published: February 2019

Until recently the subaptera-group of Phyllodromica contained only one species. The revision of the subaptera-group herein consists of the two newly described bisexual species, P. iberica and P. quadracantha, endemic to the Iberian Peninsula and a parthenogenetic species, P. subaptera (Rambur, 1838), which is widely distributed over most of the Mediterranean countries and islands. Within P. iberica three conspecific morphotypes are distinguished. The morphological characteristics of the subaptera-group are described. The species and their distributions are described and depicted. A key for the morphological determination of P. quadracantha and the morphotypes of P. iberica is given. DNA sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene are included in the species descriptions. The sequence data are suitable for species identification (DNA barcodes). A cladistic analysis of the morphological data and a phylogenetic analysis of the DNA sequences were performed to infer the phylogenetic relationships between the species of the subaptera-group.
Edoughnura rara n.gen., n.sp., an enigmatic genus of Neanurinae Collembola from the Edough Massif (Algeria)
Authors: DEHARVENG LOUIS , KROUA SALAH HAMRA , BEDOS ANNE 
Published: February 2019

A new genus and new species of Neanurinae Collembola, Edoughnura rara, is described from the Edough massif in Algeria. It combines a number of unusual or unique features : additional chaetae on head; mandible as a long, thin, ciliated lamella; chaetae Di of Th. II-III in line; tubercles Di fused on axis from Abd.III to Abd.V. Its affinities within the tribe Neanurini remains obscure.
The Ciidae (Coleoptera: Tenebrionoidea) of the Maritime Provinces of Canada: new records, distribution, zoogeography, and observations on beetle-fungi relationships in saproxylic environments
Authors: MAJKA CHRISTOPHER G. 
Published: February 2019

The Ciidae of the Maritime Provinces of Canada are surveyed. Fifteen species are now known to occur in the region, thirteen in Nova Scotia, six in New Brunswick, and two on Prince Edward Island. Ten new provincial records are reported. Seven species including Ceracis sallei Mellié, Ceracis thoracicornis (Ziegler), Cis creberrimus Mellié, Cis pistoria Casey, Cis subtilis Mellié, Malacocis brevicollis (Casey), and Orthocis punctatus (Mellié) are newly recorded in the Maritime Provinces as a whole. Cis americanus Mannerheim and Cis levettei (Casey) are newly recorded on Prince Edward Island, the first records of this family from the province.Collecting effort on Cape Breton Island, Prince Edward Island, and in New Brunswick has apparently been insufficient to fully document the ciid fauna of these areas. Some local and regional distribution patterns of ciids in the mainland of Nova Scotia and in the Maritime Provinces are suggested from the present data, but further collecting is required to confirm these. Zoogeographically, most of the region's ciids are members of either a boreal fauna (9 species) with Holarctic affinities, or a southeastern North American Nearctic fauna (5 species). The Maritime Provinces ciid fauna has representatives of five of the six known ciid host-use groups. Records of host fungi indicate that there are suitable hosts for all species of ciids found in the region in all three Maritime Provinces, indicating that ciids in the region appear not to be limited by availability of suitable host-fungi. However, Cis horridulus Casey, Cis striolatus Casey, and Cis subtilis Mellié, the three species in the Trametes host-use group, are very infrequently collected and apparently rare.Forests in Maritime Provinces have been greatly affected by forestry and disease, and such activities are known to impact fungal communities. Consequently such practices could have important repercussions for groups like the Ciidae that are reliant on fungi as both a food source and a habitat
100 Years of Progress in Forecasting and NWP Applications
Authors: Benjamin Stanley G. , Brown John M. , Brunet Gilbert , Lynch Peter , Saito Kazuo , Schlatter Thomas W. 
Published: February 2019

Abstract
Over the past 100 years, the collaborative effort of the international science community, including government weather services and the media, along with the associated proliferation of environmental observations, improved scientific understanding, and growth of technology, has radically transformed weather forecasting into an effective global and regional environmental prediction capability. This chapter traces the evolution of forecasting, starting in 1919 [when the American Meteorological Society (AMS) was founded], over four eras separated by breakpoints at 1939, 1956, and 1985. The current state of forecasting could not have been achieved without essential collaboration within and among countries in pursuing the common weather and Earth-system prediction challenge. AMS itself has had a strong role in enabling this international collaboration.
Credit Advisers, Consumer Credit and Equitable Fiduciary Obligations
Authors: Degeling Simone , Hudson Jessica 
Published: February 2019

AbstractConsumers use financial intermediaries such as brokers and other credit advisers to navigate complex financial markets and to provide guidance on credit products. In 2017 ASIC reported that ‘[b]rokers … are responsible for arranging … half of all home loans in Australia’ (Australian Securities & Investments Commission, Report 516: Review of Mortgage Broker Remuneration (2017) 8 [18]). The National Consumer Credit Protection Act 2009 (Cth) (‘Credit Regime’) regulates the conduct of such advisers including requiring disclosure of fees and some commissions. The Credit Regime also permits conflicts between the interest of the adviser and the client, provided that the adviser has in place ‘adequate arrangements to ensure … [that the client is] … not disadvantaged by any conflict of interest’ and that the conflict does not breach the adviser’s obligation to act ‘efficiently, honestly and fairly’. This article demonstrates that equitable fiduciary obligations also operate to regulate the conduct of the adviser in his or her dealings with the client. Such conflict and other conduct may breach any equitable fiduciary obligation thus exposing the adviser to equitable remedies. Equitable fiduciary obligations may thus be an as yet under-exploited avenue of protection for consumers and a concomitant zone of compliance risk for those subject to the Credit Regime.