bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measuring Circulating Complement Activation Products in Myeloperoxidase– and Proteinase 3–Antineutrophil Cytoplasmic Antibody–Associated Vasculitis
Authors: Wu Eveline Y. , McInnis Elizabeth A. , Boyer‐Suavet Sonia , Mendoza Carmen E. , Aybar Lydia T. , Kennedy Kristin B. , Poulton Caroline J. , Henderson Candace D. , Hu Yichun , Hogan Susan L. , Hu Peiqi , Xiao Hong , Nachman Patrick H. , Jennette J. Charles , Falk Ronald J. , Bunch Donna O. 
Published: June 2019

ObjectiveThere is accumulating evidence that complement activation is important in antineutrophil cytoplasmic antibody (ANCA)–associated vasculitis (AAV) pathogenesis. This study was undertaken to investigate complement activation in AAV with myeloperoxidase (MPO) positivity and AAV with proteinase 3 (PR3) positivity after determining optimal methods for measuring activated complement factors in circulation.MethodsParticipants included 98 patients with AAV (45 MPO‐ANCA positive, 53 PR3‐ANCA positive) and 35 healthy controls. Plasma was obtained from blood collected using EDTA tubes, with or without 100 μg/ml Futhan. Levels of Bb, C3a, C5a, soluble C5b–9 (sC5b–9), properdin, and C4d were measured by enzyme‐linked immunosorbent assay. Group comparisons were made using Wilcoxon's 2‐sample test. Paired data were analyzed using a matched pairs signed rank test.ResultsCompared to healthy controls, certain complement analyte levels were high in patients with active AAV with MPO positivity, including C3a (P < 0.0001), C5a (P = 0.0004), and sC5b–9 (P = 0.0007). During remission, levels of Bb (P = 0.001), C3a (P < 0.0001), and sC5b–9 (P = 0.003) were higher. Compared to healthy controls, C3a (P < 0.0001), C5a (P = 0.002), sC5b–9 (P = 0.0001), and C4d (P = 0.005) levels were higher in patients with active AAV with PR3 positivity; levels of C3a (P < 0.0001) and C4d (P = 0.007) were also higher duriing remission. There were no significant differences in any complement analyte for either ANCA serotype between patients with active disease and those with disease in remission. Among patients with paired samples, sC5‐9 levels were significantly lower during disease remission compared to active disease. C5a was significantly lower among patients with disease in long‐term remission who were not receiving therapy. For Bb, C5a, and sC5b–9, median levels and individual values were considerably higher in control and patient samples processed without Futhan compared to those processed with Futhan.ConclusionComplement activation occurs in both MPO‐positive AAV and PR3‐positive AAV. The complement activation profile differs according to disease activity and possibly ANCA serotype. Futhan reduces in vitro complement activation and provides a more accurate measurement.
Randomized clinical trial of accelerated enhanced recovery after minimally invasive colorectal cancer surgery (RecoverMI trial)
Authors: Bednarski B K , Nickerson T P , You Y N , Messick C A , Speer B , Gottumukkala V , Manandhar M , Weldon M , Dean E M , Qiao W , Wang X , Chang G J 
Published: June 2019

Abstract

Background
Minimally invasive surgery (MIS) and enhanced recovery protocols (ERPs) have improved postoperative recovery and shortened length of hospital stay (LOS). Telemedicine technology has potential to improve outcomes and patient experience further. This study was designed to determine whether the combination of MIS, ERP and a structured telemedicine programme (TeleRecovery) could shorten total 30-day LOS by 50 per cent.

Methods
This was a phase II prospective RCT at a large academic medical centre. Eligible patients aged 18–80 years undergoing minimally invasive colorectal resection using an ERP were randomized after surgery. The experimental arm (RecoverMI) included accelerated discharge on postoperative day (POD) 1 with or without evidence of bowel function and a televideoconference on POD 2. The control arm was standard postoperative care. The primary endpoint was total 30-day LOS (postoperative stay plus readmission/emergency department/observation days). Secondary endpoints included patient-reported outcomes measured by EQ-5D-5L™, Brief Pain Inventory (BPI) and a satisfaction questionnaire.

Results
Thirty patients were randomized after robotic (21 patients) or laparoscopic (9) colectomy, including 14 patients in the RecoverMI arm. Median 30-day total LOS was 28·3 (i.q.r. 23·7–43·6) h in the RecoverMI arm and 51·5 (43·8–67·0) h in the control arm (P = 0·041). There were no differences in severe adverse events or EQ-5D-5L™ score between the study arms. The BPI revealed low pain scores regardless of treatment arm. Satisfaction was high in both arms.

Conclusion
In patients having surgery for colorectal neoplasms, the trimodal combination of MIS, ERP and TeleRecovery can reduce 30-day LOS while preserving patients' quality of life and satisfaction. Registration number: NCT02613728 (https://clinicaltrials.gov).
Cistanche extracts ameliorates the neurotoxicity induced by hydrogen peroxide in new mutant DJ‐1‐transfected neuroblastoma cellular models
Authors: An Chunna , Pu Xiaoping , Wang Qi , Zhang Hongning 
Published: June 2019

AbstractIntroductionDJ‐1 mutation is a causative reason for familial Parkinson's disease (PD). Leucine166Proline (L166P) and C106S are two important DJ‐1 mutations. In this study, we established hydrogen peroxide (H2O2) induced L166P and C106S DJ‐1‐transfected neuroblastoma (SH‐SY5Y) cellular models of PD and investigated the effects of Cistanche extracts and key bioactive compounds, including acteoside, echinacoside, caffeic acid, and Cistanche total glycosides on these two models.MethodsAfter expressing FLAG‐tagged L166P and C106S DJ‐1 plasmids in Escherichia coli, the expressed plasmids were collected, treated with restriction enzyme, and identified using DNA electrophoresis. After purification, the L166P DJ‐1 and C106S DJ‐1 plasmids were separately transfected into SH‐SY5Y cells using liposomes. Transfected SH‐SY5Y cells were detected by western blotting and immunocytochemistry. Cell viability was determined using MTT assay.ResultsBoth western blotting and immunocytochemistry showed that L166P and C106S DJ‐1 were highly expressed in the transfected SH‐SY5Y cells. MTT assays showed that transfection with L166P or C106S DJ‐1 reduced the viability of SH‐SY5Y cells exposed to H2O2, as compared to untransfected SH‐SY5Y cells. In addition, Cistanche extracts and key bioactive compounds, including acteoside, echinacoside, caffeic acid, and Cistanche total glycosides, significantly inhibited the decreases of cell viability caused by H2O2 in L166P and C106S DJ‐1‐transfected SH‐SY5Y cells.ConclusionsThese findings suggest that we successfully established sensitive and stable H2O2 induced L166P DJ‐1‐ and C106S DJ‐1‐transfected SH‐SY5Y cell models of PD and Cistanche extracts may thus be useful for treating PD.
Melanoma incidence, recurrence, and mortality in an integrated healthcare system: A retrospective cohort study
Authors: Feigelson Heather S. , Powers John D. , Kumar Mayanka , Carroll Nikki M. , Pathy Arun , Ritzwoller Debra P. 
Published: June 2019

AbstractBackgroundNumerous studies have examined melanoma incidence and survival, but studies on melanoma recurrence are limited. We examined melanoma incidence, recurrence, and mortality among members of Kaiser Permanente Colorado (KPCO) between January 1, 2000 and December 31, 2015.MethodsAge‐adjusted incidence rates were computed to examine trends among KPCO members aged 21 years and older. Cox proportional hazards models were used to examine factors associated with recurrence and mortality.ResultsOur cohort included 1931 cases of invasive melanoma. Incidence rates increased over time and were higher than SEER rates; however, the increase was limited to early stage disease. In multivariable models, stage at initial diagnosis, gender, and age were associated with melanoma recurrence. Men were more likely to have a recurrence than women (adjusted hazard ratio [HR]: 1.70, 95% confidence interval [CI]: 1.19‐2.43), and for each decade of increasing age, the adjusted HR = 1.20 (95% CI: 1.06‐1.37). Factors associated with all‐cause mortality included stage (HR = 12.87, 95% CI: 6.63‐24.99, for stage IV vs stage I), male gender (HR = 1.42, 95% CI: 1.12‐1.79), older age at diagnosis, lower socioeconomic status, and comorbidity index. For melanoma‐specific mortality, results were similar, with one exception: age was not associated with melanoma‐specific death (HR = 1.09, 95% CI: 0.94‐1.25, P = 0.253).ConclusionsData derived from an insured patient population, such as KPCO, have the potential to enhance our understanding of emerging trends in melanoma. This is the first population‐based study in the United States to examine patient characteristics associated with risk of recurrence. Men have an increased risk of both recurrence and death, and thus may benefit from more intensive follow‐up than women.
LncRNA HCP5 promotes triple negative breast cancer progression as a ceRNA to regulate BIRC3 by sponging miR‐219a‐5p
Authors: Wang Lihong , Luan Tian , Zhou Shunheng , Lin Jing , Yang Yue , Liu Wei , Tong Xiao , Jiang Wei 
Published: June 2019

AbstractEmerging evidence has suggested that long noncoding RNAs (lncRNA) involved in the development and progression of cancer. Triple negative breast cancer (TNBC) was an aggressive type of breast cancer with high rates of cancer recurrence and metastasis. The pathogenesis of TNBC is largely unknown. Recent studies suggested that lncRNA HCP5 plays an important role in carcinogenesis. The purpose of this study was to examine the function and mechanism of HCP5 in TNBC. We observed that HCP5 was upregulated in TNBC cell lines and specimens. HCP5 knockdown induced TNBC cell apoptosis, and inhibited cell proliferation and orthotopic xenograft tumor growth. RNA sequencing and antibody array suggested that HCP5 achieves its functions through regulating apoptosis pathway. Bioinformatics, luciferase and RIP experiments proved that both HCP5 and BIRC3 could competitively bind to miR‐219a‐5p. Increased BIRC3 and decreased miR‐219a‐5p were observed in TNBC tissues and cell lines. We then performed gain‐ and loss‐of‐function studies as well as rescue experiments in TNBC cells. The decrease of proliferation and migration due to HCP5 knockdown could be rescued when miR‐219a‐5p inhibitor or BIRC3 was transfected and vice versa. Our study suggested that lncRNA HCP5 promotes TNBC progression as a ceRNA to regulate BIRC3 by sponging miR‐219a‐5p. In a word, we revealed a new signaling pathway to mediate TNBC, and provided HCP5 as a new target for improving treatment of TNBC.
Targeting Bacterial Biofilm: A New LecA Multivalent Ligand with Inhibitory Activity
Authors: Palmioli Alessandro , Sperandeo Paola , Polissi Alessandra , Airoldi Cristina 
Published: June 2019

AbstractBiofilm formation by bacterial pathogens is a hallmark of chronic infections and is associated to increased antibiotic tolerance that makes pathogens difficult to eradicate with conventional antibiotic therapies. Infections caused by Pseudomonas aeruginosa are of great concern, especially for immunocompromised and cystic fibrosis patients. P. aeruginosa lectins LecA and LecB are virulence factors and play a key role in establishing biofilm; therefore, inhibition of the function of these proteins has potential in dismantling the bacterium from the protective biofilm environment and in restoring the activity of antibiotics. Here, we report the NMR characterization of the binding of a galactose‐based dendrimer (Gal18) to LecA. Moreover, we demonstrate the activity of the Gal18 molecule in inhibiting P. aeruginosa biofilm formation in vitro.
Novel Non‐toxic and Non‐hazardous Solvent Systems for the Chemistry of Indoles: Use of a Sulfone‐containing Brønsted Acid Ionic Liquid Catalyst in Butyl Acetate
Authors: El‐Harairy Ahmed , Yiliqi  , Yue Meie , Fan Weigang , Popowycz Florence , Queneau Yves , Li Minghao , Gu Yanlong 
Published: June 2019

AbstractDipolar and aprotic solvents are often required in acid‐catalyzed reactions, however, many of them are listed in solvent selection guides as not‐recommended due to their toxicity or explosivity. A catalytically functionalized ionic liquid, namely a sulfone‐containing imidazolium‐based Brønsted acid ionic liquid, was synthesized. It alleviates the detrimental effect of classical dipolar aprotic solvents because this ionic liquid integrates the function of a dipolar aprotic solvent able to stabilize carbocation intermediates and the activating effect of a strong Brønsted acid on electrophiles. The use of this tailor‐made ionic liquid was exemplified in some transformation reactions of indoles, which proceeded with high yield and selectivity using a green and an industrially acceptable solvent, butyl acetate. The recyclability of the ionic liquid catalyst was also demonstrated.
Glycopeptides by Linch‐Pin C−H Activations for Peptide‐Carbohydrate Conjugation by Manganese(I)‐Catalysis
Authors: Wang Wei , Subramanian Parthasarathi , Martinazzoli Oscar , Wu Jun , Ackermann Lutz 
Published: June 2019

AbstractLate‐stage C−H glycosylations of structurally complex amino acids and peptides were accomplished by means of racemization‐free manganese(I)‐catalyzed C−H activation. Thus, glycosylative modifications proved to be viable by a linch‐pin approach, featuring chemo‐ and site‐selective C−H transformations. The peptide–saccharide conjugation provided modular access to structurally complex glycopeptides, likewise enabling the assembly of fluorescent‐labelled glycopeptides.
Diphospha‐Ureas from the Phosphaketene Ph3GePCO
Authors: Szkop Kevin M. , Jupp Andrew R. , Razumkov Hlib , Xu Maotong , Stephan Douglas W. 
Published: June 2019

AbstractThe phosphaketene Ph3GePCO is shown to react with the phosphide KP(tBu)2 to generate the anion [Ph3GePC(O)P(tBu)2]− 1. This species reacts with CH3I or ClGePh3 to give the dissymmetric diphospha‐ureas (tBu)2PC(O)P(GePh3)(CH3) 2 and (Ph3Ge)2PC(O)P(tBu)2 3 respectively. Sequential treatment of 2 with a base and CH3I affords a route to (tBu)2PC(O)P(CH3)2 5. These species are products of the first modular diphospha‐urea synthesis. The subsequent thermal and photochemical reactivity of these species was also probed and described.
New approach to allocation planning of many‐task workflows on clouds
Authors: Gerhards Michael , Sander Volker , Živković Miroslav , Belloum Adam , Bubak Marian 
Published: June 2019

SummaryExperience has shown that a priori created static resource allocation plans are vulnerable to runtime deviations and hence often become uneconomic or highly exceed a predefined soft deadline. The assumption of constant task execution times during allocation planning is even more unlikely in a cloud environment where virtualized resources vary in performance. Revising the initially created resource allocation plan at runtime allows the scheduler to react on deviations between planning and execution. Such an adaptive rescheduling of a many‐task application workflow is only feasible, when the planning time can be handled efficiently at runtime. In this paper, we present the static low‐complexity resource allocation planning algorithm (LCP) applicable to efficiently schedule many‐task scientific application workflows on cloud resources of different capabilities. The benefits of the presented algorithm are benchmarked against alternative approaches. The benchmark results show that LCP is not only able to compete against higher complexity algorithms in terms of planned costs and planned makespan but also outperforms them significantly by magnitudes of 2 to 160 in terms of required planning time. Hence, LCP is superior in terms of practical usability where low planning time is essential such as in our targeted online rescheduling scenario.
Microwave‐Assisted Synthesis of Sulfurated Heterocycles with Herbicidal Activity: Reaction of 2‐Alkynylbenzoic Acids with Lawesson's Reagent
Authors: Giofrè Salvatore V. , Mancuso Raffaella , Araniti Fabrizio , Romeo Roberto , Iannazzo Daniela , Abenavoli Maria Rosa , Gabriele Bartolo 
Published: June 2019

AbstractThe reactivity of 2‐alkynylbenzoic acids toward Lawesson's reagent (LR) under microwave irradiation (300 W, 100 °C, CH2Cl2) was assessed. It was found that, depending on reaction conditions, either a dithionation‐ or a monothionation‐cycloisomerization process takes place with formation of important sulfurated heterocycles. In particular, using 1 equivalent of LR for 1 h, dithionation occurred, with formation of benzo[c]thiophene‐1(3H)‐thiones or 1H‐isothiochromene‐1‐thiones, while with 0.5 equiv. of LR for 10–30 min, monothionated products were selectively obtained (benzo[c]thiophen‐1(3H)‐ones or 1H‐isothiochromen‐1‐ones). The regiochemical output of the process strongly depended on the substitution pattern of the starting 2‐alkynylbenzoic acid derivatives. These compounds were also assayed as potential herbicides by assessing their phytotoxic activity on seedling growth and development of the model species Arabidopsis thaliana. All compounds, to different extents, influenced the morpho‐physiological parameters that were monitored; in particular, the fresh weight (FW) was significantly affected, with ED50 values ranging from 4.81–63.7 μM.
The curvilinear and time‐lagging impact of sustainability performance on financial performance: Evidence from Germany
Authors: Nuber Claudio , Velte Patrick , Hörisch Jacob 
Published: June 2019

AbstractBased on the stakeholder‐agency theory, this study empirically investigates the impact of sustainability performance on financial performance. We rely on a sample from the German DAX30, MDAX, and TecDAX from 2008 to 2017. In contrast to former studies concentrating on the German market that tested a direct linear relationship between sustainability performance and financial performance, a time‐lagging and curvilinear regression analysis was carried out, and evidence of a U‐shaped relationship was found. This implies that sustainability management aiming at increasing financial performance should proactively strive for very high levels of corporate sustainability to meet the needs of investors and further stakeholders.
An online resource allocation algorithm to minimize system interference for inband underlay D2D communications
Authors: Hassan Md Yeakub , Hussain Faisal , Hossen Md Sakhawat , Choudhury Salimur 
Published: June 2019

SummaryThis paper addresses the research question of total system interference minimization while maintaining a target system sum rate gain in an inband underlay device‐to‐device (D2D) communication. To the best of our knowledge, most of the state of the art research works exploit offline resource allocation algorithms to address the research problem. However, in Long‐Term Evolution (LTE) and beyond systems (4G, 5G, or 5G+), offline resource allocation algorithms do not comply with the fast scheduling requirements because of the high data rate demand. In this paper, we propose a bi‐phase online resource allocation algorithm to minimize the total system interference for inband underlay D2D communication. Our proposed algorithm assumes D2D pairs as a set of variable elements whereas takes the cellular user equipment (UEs) as a set of constant elements. The novelty of our proposed online resource allocation algorithm is that it incurs a minimum number of changes in radio resource assignment between two successive allocations among the cellular UEs and the D2D pairs. Graphical representation of the simulation results suggests that our proposed algorithm outperforms the existing offline algorithm considering number of changes in successive allocation for a certain percentage of sum rate gain maintaining the total system interference and total system sum rate very similar.
Electric vehicles with small batteries and high‐efficiency on‐board electricity production
Authors: Boretti Alberto 
Published: June 2019

AbstractData of plug‐in hybrid electric vehicles (PHEV), that includes both traditional hybrids electric vehicles (HEV) with the additional opportunity to recharge the battery by plugging into an electric outlet such as the Toyota Prius Prime, and battery electric vehicles (BEV) with range extender such as the BMW i3 REx, demonstrate the need of a less constrained research and development to optimize the design of electric vehicles with range extender for better energy economy and range. BEV with smaller, rather than larger, batteries, and onboard high‐efficiency electricity production, are superior economically and environmentally, until the times the electricity production will be overwhelming renewable, there will be a widespread recharging infrastructure, materials to produce the batteries will be abundant and ethically sourced, and the disposal of the batteries will be properly addressed. A hypothetical BMW i3 with a 30 Ah battery pack, and a high‐efficiency ICE, may have an MPGe (miles‐per‐gallon equivalent) working electric of 127 miles, a MPGe between 62 and 127 while sharing the energy supply, and a MPGe of 62 only using the gasoline energy supply.
Molecular evolution across developmental time reveals rapid divergence in early embryogenesis
Authors: Cutter Asher D. , Garrett Rose H. , Mark Stephanie , Wang Wei , Sun Lei 
Published: June 2019

AbstractOntogenetic development hinges on the changes in gene expression in time and space within an organism, suggesting that the demands of ontogenetic growth can impose or reveal predictable pattern in the molecular evolution of genes expressed dynamically across development. Here, we characterize coexpression modules of the Caenorhabditis elegans transcriptome, using a time series of 30 points from early embryo to adult. By capturing the functional form of expression profiles with quantitative metrics, we find fastest evolution in the distinctive set of genes with transcript abundance that declines through development from a peak in young embryos. These genes are highly enriched for oogenic function and transient early zygotic expression, are nonrandomly distributed in the genome, and correspond to a life stage especially prone to inviability in interspecies hybrids. These observations conflict with the “early conservation model” for the evolution of development, although expression-weighted sequence divergence analysis provides some support for the “hourglass model.” Genes in coexpression modules that peak toward adulthood also evolve fast, being hyper-enriched for roles in spermatogenesis, implicating a history of sexual selection and relaxation of selection on sperm as key factors driving rapid change to ontogenetically distinguishable coexpression modules of genes. We propose that these predictable trends of molecular evolution for dynamically expressed genes across ontogeny predispose particular life stages, early embryogenesis in particular, to hybrid dysfunction in the speciation process.