bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of postprocedural permanent pacemaker implantation on clinical outcomes after transcatheter aortic valve replacement: A systematic review and meta-analysis
Authors: Xi Ziwei , Liu Tong , Liang Jing , Zhou Yujie , Liu Wei 
Published: August 2019

Abstract
Background: The incidence of conduction disturbances requiring permanent pacemaker (PPM) implantation following transcatheter aortic valve replacement (TAVR) have remained a common concern. The purpose of this study was to evaluate the impact of postprocedural PPM implantation following TAVR on clinical outcomes.
Methods: We performed a systematic search in PubMed and EMBASE databases for studies that reported raw data on clinical outcomes of patients with and without PPM implantation after TAVR and followed up patients for 10 months or longer. The primary endpoint was all-cause death. The secondary endpoints were cardiovascular death, heart failure and a composite of stroke and myocardial infarction (MI).
Results: Data from 20 studies with a total of 21666 patients undergoing TAVR, of whom 12.5% required PPM implantation after intervention, were analysed and the mean duration follow-up was 16.9 months. The rate of PPM ranged from 6.2% to 32.8% among different studies. A total of 6753 (31.2%) patients underwent TAVR with self-expandable prosthesis and 14913 (68.8%) with balloon-expandable prosthesis. The incidence of postprocedural PPM implantation was higher with the self-expandable prosthesis (n=1717, 25.4%) compared with the balloon-expandable prosthesis (n=996, 6.7%). PPM after TAVR was associated with a higher risk of all-cause death (RR: 1.13; 95% CI: 1.01-1.25; P=0.03) but not incidence of stroke and MI (RR: 0.85; 95% CI: 0.64-1.13; P=0.27).
Conclusions: In patients undergoing TAVR, the PPM implantation after intervention was associated higher all-cause mortality but not cardiovascular mortality, heart failure and stroke or MI, which remain an unsolved issue of TAVR.
A comparative study of Long Interspersed Element-1 Protein Immunoreactivity in Cutaneous Malignancies
Authors: Zolfaghari Mohammad Ali , Karimi Abbas , Kalantari Elham , Korourian Alireza , Ghanadan Alireza , Kamyab Kambiz , Esmaili Nafiseh , Razavi Amir Nader Emami , Madjd Zahra 
Published: August 2019

Abstract
Abstract
Background
Skin cancer is the most common cancer worldwide and commonly classified into malignant melanoma (MM) and Nonmelanoma skin cancers (NMSCs), which mainly include basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). The extent to which Long Interspersed Element-1 (LINE-1, L1) ORF1p is expressed in cutaneous malignancies remains to be evaluated. The aim of this study was to assess LINE-1 ORF1p immunoreactivity in various skin cancer subtypes.
Method
The expression level of LINE-1 ORF1p was evaluated in 95 skin cancer specimens comprising 36 (37.9%) BCC, 28 (29.5%) SCC, and 31 (32.6%) melanoma using the tissue microarray (TMA) technique. Then the association between expression of LINE-1 encoded protein and clinicopathological parameters were analyzed.
Results
We showed that LINE-1 ORF1p expression level was substantially higher in BCC and SCC patients compared with melanoma samples (p < 0.001). BCC cases had higher LINE-1staining intensity scores compared with SCC cases (p = 0.004). In SCC samples lower level of LINE-1 ORF1p expression was associated with age lower than the mean (p = 0.041), while no significant correlation was found between LINE-1 ORF1p expression and other clinicopathological parameters (all p>0.05).
Conclusion : According to our observation, LINE-1 ORF1p immunoreactivity in various skin tumor subtypes extends previous studies of LINE-1 expression in different cancers. LINE-1ORF1p overexpression in NMSCs compared with MM can be considered with caution as a tumor-specific antigen for NMSCs.
Keywords: Skin Neoplasms; Retroelements; LINE-1 ORF1p; Immunohistochemistry; Tissue microarray; Biomarker
Exosomal miR-1290 promotes angiogenesis in hepatocellular carcinoma via targeting SMEK1
Authors: Wang Qiong , Wang Guanwen , Niu Lianjie , Zhao Shaorong , Li Jianjun , Zhang Zhen , Jiang Huimin , Zhang Quansheng , Wang Hang , Sun Peiqing , Yang Shuang , Xiang Rong 
Published: August 2019

Abstract
Abstract
Background: Hepatocellular carcinoma (HCC), the most common primary liver cancer, rely on the formation of new blood vessel for growth and frequent intrahepatic and extrahepatic metastasis. Therefore, it is important to explore the underlying molecular mechanisms of tumor angiogenesis of HCC. Recently, microRNAs have been shown to modulate angiogenic processes by modulating the expression of critical angiogenic factors. However, the potential roles of tumor-derived exosomal microRNAs in regulating tumor angiogenesis remain to be elucidated.
Methods: MiRNome sequencing was performed to uncover the miRNAs that are dysregulated in HCC patient serum-derived exosomes. Expression levels of miR-1290 in tissues and cells were determined by quantitative real-time PCR. The effect of mir-1290 on proliferation was evaluated by CCK-8 assay. The angiogenic ability of cells were determined by transwell, wound-healing, tube formation and matrigel plug assays. SMMC-7721 xenograft tumor model was established in NOD-SCID nude mice using miR-1290 and NC antagomirs to determin the angiogenic effect of mir-1290 in vivo. Target protein expression was determined by western blotting. Dual luciferase reporter assay was performed to confirm the action of miR-1290 on downstream target genes including SMEK1. Results are reported as means ± S.D. and differences were tested for significance using 2-sided Student’s t-test.
Results: In this study, our miRNome sequencing demonstrated that miR-1290 was overexpressed in HCC patient serum-derived exosomes, and we found that delivery of miR-1290 into human endothelial cells enhanced their angiogenic ability. Our results further revealed that SMEK1 is a direct target of miR-1290 in endothelial cells. MiR-1290 exerted its pro-angiogenic function, at least in part, by inhibiting the VEGFR2 signaling pathway in a SMEK1-dependent manner.
Conclusions: Collectively, our findings provide evidence that miR-1290 is overexpressed in HCC and promotes tumor angiogenesis via exosomal secretion, implicating its potential role as a therapeutic target for HCC.
Is the effect of Decitabine limited to hypomethylation and DNMTs?
Authors: Dalvand Sina , Namdari Amin , Alemi Ashraf , Meshkibaf Mohammad Hassan , Setayesh Sam , Karamallah Mojtaba Haghi , Farrokhifar Mohammad 
Published: August 2019

Abstract
Background: Histone modifications play a crucial role in chromatin structure. Among enzymes, which regulate these processes, histone deacetylases (HDACs) can remove acetyl groups from histone tails, thus increasing their interaction with DNA and leading to chromatin condensation. 5-Aza-2′-deoxycytidine (AZad) or Decitabine is a potent hypomethylating agent that incorporates into DNA and traps DNA methyltransferase in the form of a covalent protein–DNA adduct. Azad, not only change the gene expression through demethylation of the gene's promoter, but it also can change gene expression independently from DNA demethylation. So, the present study was to distinguish whether AZad in addition to inhibitory effects on DNA methyltransferase, can change HDAC3 and HDAC7 mRNA expression in NALM-6, HL-60 cancer cell lines.
Methods: HL-60, NALM-6 and normal cells were cultured, and the treatment dose of the AZad was obtained (1µM) by the MTT test. Finally, HDAC3 and HDAC7 mRNA expression were measured by Real Time PCR in HL-60 and NALM-6 cancerous cells before and after treatment. In addition, HDAC3 and HDAC7 mRNA expression in un-treated HL-60 and NALM-6 cancerous cells were compared to the normal cells.
Results: Our result revealed that expression of HDAC3 and HDAC7, in HL-60 and NALM-6 cells increases as compared to normal cells. After treatment of HL-60 and NALM-6 cells with AZad, HDAC3 and HDAC7 mRNA expression were decreased significantly.
Conclusions: Our data showed, the effects of AZad are not limited to direct hypomethylation of DNMTs but it can indirectly affect other epigenetic factors, such as HDACs activity, through converging pathways.
Keywords: HDAC3 ; HDAC7 ; HL-60; NALM-6 ; Decitabine ; AZad
Can we perform adjuvant chemotherapy alone in stage IB/IIA cervical cancer meeting Sedlis criteria?
Authors: Li Meng , Gu Ying , Zheng Yawen , Yang Xiaohang , Cui Baoxia , Wu Bin , Yang Xingsheng 
Published: August 2019

Abstract
Background: The aim of this study was to evaluate the effectiveness of chemotherapy alone as postsurgical adjuvant therapy on survival of patients in stage IB/IIA cervical squamous cell carcinoma meeting Sedlis criteria.
Methods: We retrospectively reviewed the records of 127 patients satisfying Sedlis criteria after radical surgery for stage IB/IIA cervical cancer from January 2009 to December 2013. Of these, 65 patients received radiotherapy (RT)/chemoradiotherapy (CRT), 62 patients received chemotherapy (CT) alone. Both the treatment modalities were compared on 5-year overall survival (OS), disease-specific survival (DSS), recurrence rates and side effects.
Results: Median follow-up time for surviving patients was 75 months. The 5-year OS was similar in both the groups with 85.5% in the CT and 87.7% in the RT/CRT group, respectively, (p=0.727). Likewise, there was no significant difference in 5-year DSS between the two arms (CT: 87.1% vs. RT/CRT: 90.8%; P=0.548). Rate and the pattern of recurrence were not different between the groups. Acute toxicity was more frequent in the CT group, but was statistically insignificant (P>0.05). The incidences of urinary disturbance (P=0.003) and lymphedema (P=0.041) were fewer in the CT group.
Conclusions: Adjuvant CT alone for early-stage cervical cancer patients who meet the Sedlis criteria might be a good alternative to RT/CRT.
Prognostic value of pretreatment D-dimer level in small-cell lung cancer: a meta-analysis
Authors: Wang Yan , Li Jialong , Wu Yanming , Li Jue , Che Guowei 
Published: August 2019

Abstract
Purpose To determine the prognostic significance of pretreatment D-dimer level in predicting clinical outcomes, such as the overall survival (OS) and progression-free survival (PFS), of patients with small cell lung cancer (SCLC).
Methods A systematic search in PubMed, Web of Science, EMBASE, Cochrane Library, CNKI, SinoMed, Wanfang and VIP databases was performed to identify available studies. The pooled hazard ratios (HRs) with 95% confidence intervals (CIs) were applied to assess the association of pretreatment D-dimer level with prognosis of SCLC patients. All statistical analyses were conducted via the STATA 12.0 version software.
Results A total of 7 studies involving 964 patients were included in this meta-analysis and all patients were from China. The results showed that elevated pretreatment D-dimer level was significantly correlated with worse OS(HR=1.90, 95% CI: 1.55-2.34, P <0.001) and PFS (HR=1.52, 95% CI: 1.24-1.85, P ＜0.001). Subgroup analyses based on the treatment, D-dimer cut-off, detection method and source of HR were also performed to further verify the prognostic value of pretreatment D-dimer level in SCLC.
Conclusions Pretreatment blood concentration of D-dimer may deserve as a reliable factor to predict prognosis of Chinese patients with SCLC. More well-designed prospective studies with big samples are still needed to verify our findings.
Mortality among cancer patients within 90 days of therapy in a tertiary hospital of Tanzania: is our pre therapy screening effective?
Authors: Changalucha Marygoreth J , Mushi MARTHA F. , Kabangila Rodrick , Silago Vitus , Likonda Beda , Mshana Stephen E. 
Published: August 2019

Abstract
Background

A high mortality has been reported during the first ninety days of cancer therapy and is more pronounced in patients with febrile neutropenia. Bugando Medical Center, oncology department offers cancer diagnosis and treatment services to the population of lake-zone of Tanzania with limited data on the 90 days outcome. Here, we report the 90 days mortality and factors associated with it among cancer patients attending oncology department of the tertiary hospital in Tanzania.

Methodology

Enrolled participants underwent baseline physical examinations and functional status assessment using Karnofsky score. On each clinic visit, FBP was taken and patients were investigated for infections. Data were entered in Microsoft Excel and analyzed using STATA version 13.

Results

A total of 102 participants were enrolled. Their median age was 50 years [38-60]. The majority of study participants were females 76(75%) and 82(80.4%) had primary school education. The majority of patients had solid cancer 96(94.1%). A total of 12 (11.8%) patients died within 90 days of starting therapy. Having low hemoglobin level before starting cancer therapy (p=0.001), having Karnofsky score below 80% (p=0.001) and using 5-fluorouracil containing therapy (p=0.004) were found to be associated with mortality within 90 days of therapy among cancer patients.

Conclusion

About 10% of cancer patients die within 90 days of therapy. Anemic patients, with poor performance status and on 5-Fluorouracil regimen are more likely to die within 90 days of cancer therapy.
The Response of Second Line Chemotherapy Platinum Resistant Ovarian Cancer in Sudan: 2013-2017
Authors: Alshykh Mohammed Altyb , Essa Mohammed Elmujtba Adam , Suleiman Yousra Abdelmoniem , Osman Sherihan Mohammed Elkundi , Hussein Mustafa Mohamed Ali , Mohamed Sadia Kamal Albadawi , Abdelrahim Shiemaa Emad , Osman Leena Habeeb Allah , Mohamed Saja Hassan , Rahama Salma Ismaeel , Ali Saneyya Alsir , Abdullahi Sadiya Aminu , Musa Suha Abdallah , Ahmed Abdelkareem A. 
Published: August 2019

Abstract
Background

Ovarian carcinoma is the fifth leading cause of cancer death worldwide. The tumor mostly associated with variant factors such as advance age, early menstruation and gene association. We aimed to highlight the effectiveness of the second line chemotherapy in the management of ovarian cancer patients in Sudan.Methods

The data were collected from the hospital patient’s records for five years period of time, included 62 patients with ovarian cancer who is treated by the second line platinum resistant chemotherapy.Result

The peak prevalence of the patients was found in Al-Gazeera state, and the least in Al-Gadarif state. Age group above 55 years was the most affected group. The vast majority of patients showed partially mass disappearing, completes and continue growing tumor respectively. All patients received variant cycles of chemotherapy as fellow 3, 6, 2, 4, 5, 1 cycle respectively. 79.4% of the patients had achieved the normal value of cancer antigen 125 (CA125) levels after the treatment. In 50% of patients the cancer recurred after 1-2 months, 32.2% after 3-4 months and 17.8% after 5-6 months. The serous adenocarcinoma was found to be the most histological type in all the patients and the least two types were observed are cell carcinoma and serous papillary.Conclusion

Our findings suggested that, Al-Gazeera state citizens were more vulnerable to resist the first line chemotherapy than others. The Sudanese patients with ovarian cancer may have better response to Gemzar.
Detection of extended-spectrum beta-lactamase-producing E. coli and Klebsiella spp in effluents of different hospitals sewage in Biratnagar, Nepal
Authors: Mahato Sanjay , Mahato Ajay , Pokharel Elina , Tamrakar Ankita 
Published: August 2019

Abstract
ObjectiveThis study was aimed to determine prevalence and resistance pattern like multidrug resistant (MDR) or ESBL nature of E. coli and Klebsiella spp from various sewage drain samples with an idea to deliver baseline information that could be utilized for defining guidelines for the treatment of hospital sewages.ResultsOf 10 sewage samples analyzed, 7 (70%) contained E. coli while 6 (60%) contained Klebsiella. Except one sample, all positive samples contained both E. coli and Klebsiella spp. E. coli isolates were resistant to ampicillin, amoxicillin, cefoxitin, cefuroxime, and cefpodoxime; while 85.7% were resistant to amoxicillin/clavulanate, ceftazidime, cefotaxime and ceftriaxone. 71.4%, 57.1%, 42.9%, and 28.6% were resistant to aztreonam, trimethoprim/sulfamethoxazole, nitrofurantoin, and gentamicin. Most were sensitive to chloramphenicol, ofloxacin, ciprofloxacin, and azithromycin. 85.7% and 57.1% of E. coli were MDR and ESBL isolates respectively. Klebsiella were resistant to ampicillin, amoxicillin, and amoxicillin/clavulanate. 83.4% of Klebsiella were resistant to cefoxitin. 66.7% of strains were resistant to cefuroxime, ceftazidime, cefotaxime, ceftriaxone, and cefpodoxime. Klebsiella showed 50% resistant to aztreonam and trimethoprim/sulfamethoxazole, while 33.3% were resistant to chloramphenicol, nitrofurantoin, ofloxacin, and ciprofloxacin. Klebsiella were sensitive to azithromycin and gentamicin. 66.7% and 33.3% of Klebsiella were MDR and ESBL isolates respectively.
Risk Factors Associated with Silicosis among Zambian Copper Mine Workers: A Retrospective Case Record Review
Authors: Zimba Japhet , Dambe Rosalia , Siulapwa Yolam , Musonda Patrick 
Published: August 2019

Abstract
Background: Silicosis is the most important occupational disease worldwide and is regarded as a major public health challenge in developing countries. The disease is mainly related to exposure to crystalline silica dust, and once the disease has been established, no treatment exists. There is less focus on other factors that are related to one acquiring the disease among the people at greater risk of developing silicosis. The main aim of this study was to assess the risk factors associated with developing silicosis among Konkola Copper Mine workers in Zambia.
Methods: A retrospective case review study was used, consisting of 168 Konkola Copper Mine Workers. A data extraction checklist was used to collect data on miners from the occupational hygiene silicosis registers. Data was analyzed using STATA version 12.0; a quantile-quantile plot was used to test for normality of continuous variables such as age and a chi-squared test was used to ascertain association with development of silicosis. Adjustment for the effect of other factors was computed using an investigator led stepwise multiple logistic regression method.
Results: For each year increase in length of service, Konkola Copper Miners are 1.95 times more likely to develop silicosis adjusting for other factors such as business unit, job category (95% CI 1.92-1.99, p=0.033). Miners working in the production areas were highly susceptible to developing silicosis compared to those working in other areas. Within the production area, miners working underground represented 38% of silicosics, while those working at open pit only had 10%. Miners belonging to Konkola and Nchanga business unit were 5.42 and 5.96 times more likely to develop silicosis compared to those working in other units respectively.
Conclusions: As a result of the statistical analysis, our findings suggest that the factors considered best predictors of silicosis were length of service, business unit and the job category to which the miners belonged to (P-values 0.033, 0.001, <0.001 respectively). There is need to reduce the length of service that individuals work underground, strengthen control measures regarding utilization of personal protective equipment, health education programs, frequent job rotations (for unskilled labour) and adherence to periodical medical examinations.
Pregnancy outcome of patients with septic shock complicating pregnancy: a retrospective study
Authors: Li Manyu , Yin Jie 
Published: August 2019

Abstract
Background: To detect the maternal and neonatal outcomes of late pregnant patients complicated with sepsis shock. Methods: The medical records of all late pregnant patients (>=28 weeks) complicated with septic shock during January 2011 and November 2018 in Peking Union Medical College Hospital were retrospectively reviewed. Pertinent literature was identified by searching PubMed and EMBASE. Results: A total 9 patients with an average age of 26.11±6.9 years were included in this study. And the mean gestational age at diagnosis of septic shock was 33 weeks plus 1 day. 87.5% of patients accepted emergency delivery ,in which cesarean section was the main delivery mode. 7 patients underwent emergency delivery (including 3 cases of fetal death) and 3 cases of live births (including 1 case of mild neonatal asphyxia). The total maternal mortality rate was 33.3 percent,and neonatal death rate was 55.6%. Only 1 patient continued pregnancy after disease recovery. 15 published case reports were reviewed. The reported maternal and perinatal death rates were 20% and 46.7% respectively. 2 patients could continue pregnancy after treatment. Group A streptococcus was considered as the main pathogenic bacteria. Conclusions: Late pregnancy complicated with septic shock was associated with high maternal and fetal mortality. Emergency delivery was advised in most of patients. And cesarean section was the main mode of delivery.
Mediating Effects of Metacognitive Learning Strategies in the Relationship between Learning-related Emotions and Academic Self-efficacy with Academic Performance in Medical Students
Authors: Hayat Ali Asghar , Shatery Karim , Amini Mitra , Shokrpour Nasrin 
Published: August 2019

Abstract
Recognition of the factors affecting the medical students’ academic success is one of the most important challenges and concerns in medical schools. Hence, this study aimed to investigate the mediating effect of metacognitive learning strategies in the relationship between learning-related emotions and academic self-efficacy with academic performance in medical students. Methods: The present study was carried out on 279 students of medicine studying in Shiraz University of Medical Sciences. The students filled out three questionnaires: academic emotions (AEQ), metacognitive learning strategies, and academic self-efficacy questionnaires. The data were analyzed using SPSS and Smart PLS3. Results: The results of structural equation modeling revealed that the students’ self-efficacy has an impact on their positive emotions and metacognitive learning strategies, and these in turn affect the students’ academic performance. Moreover, positive emotions influence the metacognitive learning strategies which in turn mediate the effect of positive emotions on the academic performance. Discussion: The results of this study revealed that metacognitive strategies can play a mediating role in the students’ self-efficacy, positive emotions, and academic performance. Therefore, in case we can strengthen the students’ self-efficacy and their positive academic emotions, their performance will improve.