bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Robust Model for Hydrogen Supply Chain Network Design in China Under Renewable Energy Uncertainty
Authors: Xu Jiaqi , Li Qiaofeng , Zheng Li 
Published: November 2024

Abstract
The increasing demand for new energy sources in China, coupled with the abundant waste, has prompted the exploration of converting excess renewable energy into hydrogen through electrolyzers. However, the uncertainty surrounding renewable energy supply, its remote distribution, and regional imbalance with demand pose significant challenges for designing and planning an integrated hydrogen supply chain. This paper addresses this challenge by proposing a two-stage robust optimization model based on ellipsoidal uncertainty sets. We derive a robust approximation model and develop an algorithm using generalized Benders decomposition to solve the resulting model. Extensive numerical experiments demonstrate the superior performance of the proposed algorithm compared to CPLEX. Additionally, a case study utilizing real data from China is presented to showcase the practicality and effectiveness of the proposed model. Finally, we draw conclusions and highlight potential avenues for future research.
Evaluation Method and Performance Indices for the Motion/Force Constrainability of Overconstrained Parallel Manipulators Without Actuation Redundancy
Authors: Xu Lingmin , Ye Wei , Li Qinchuan 
Published: November 2024

Abstract
Overconstrained parallel manipulators (PMs) without actuation redundancy have been widely used in various applications, such as the parallel module of the Exechon robot for workpiece machining. However, existing methods are inadequate for describing the motion/force constraint performance of nonredundant and overconstrained PMs (NOPMs) because they do not reasonably consider the influence of the overconstrained structure. This performance is crucial for evaluating the capability to resist external loads, which is of great significance for applications requiring high stiffness and precision. To address this issue, this paper proposes a new evaluation method for the motion/force constrainability of NOPMs. First, based on the four wrench and twist systems of limbs, the output constraint performances of all the single-degree-of-freedom (DOF) mechanisms under different cases can be obtained. These single-DOF mechanisms are constructed by selectively “losing” some constraint wrench screws and “locking” all the transmission wrench screws of the original overconstrained PM, which is the key step in the establishment of this method and achieved using the ergodic method. Then, the output and input constraint performance indices in a certain configuration can be calculated, followed by the definition of a local constraint index to evaluate the motion/force constrainability of the NOPM. Notably, all the proposed indices are characterized by coordinate independence. Finally, two PMs and their corresponding overconstrained structures are simulated to demonstrate the correctness of the proposed evaluation method. The evaluation strategy can also be used for the optimal design of the NOPMs in the future.
A New Nonlinear Time-Domain Flutter Analysis Approach for Distorted Flows
Authors: Corral Roque , Rodríguez-Blanco Salvador , Chennuru Venkata Y. T. , Vahdati Mehdi , Zhao Fanzhou 
Published: November 2024

Abstract
This paper aims to establish an efficient and accurate computational fluid dynamic (CFD) method for calculating the flutter stability of fan blades in the presence of inlet distortion due to crosswind. Due to the asymmetry of the flow and interaction of fan and distortion, this type of analysis will require a whole assembly computational model. Therefore, reducing the computational cost needed to obtain accurate aerodynamic damping values is paramount. Mode-tracking free-flutter analyses are computationally expensive and troublesome due to the presence of many frequencies in the response signals and, hence, require long physical times to converge the aerodynamic damping. Moreover, the unsteady flow field associated with the distortion can be more significant than the one due to flutter, giving rise to additional numerical difficulties. On the other hand, the energy method approach is commonly performed for a single nodal diameter (ND) at a time, requiring many computations to establish the least stable ND. This paper proposes an alternative approach based on the multi-ND energy method. The underlying hypothesis is that the unsteady pressure scales linearly with the blade displacements, and the scattering of circumferential modes due to the distortion is negligible. The general method presented here is general and can be used to compute the aerodynamic damping for other types of distortion (such as downstream distortions due to pylon and structure).
Population pharmacokinetic analyses for sulbactam–durlobactam using Phase 1, 2, and 3 data
Authors: Cammarata Anthony P. , Safir M. Courtney , Trang Michael , Larson Kajal B. , O'Donnell John P. , Bhavnani Sujata M. , Rubino Christopher M. 
Published: November 2024

ABSTRACT

Sulbactam–durlobactam is a β-lactam/β-lactamase inhibitor combination approved in the United States for the treatment of hospital-acquired and ventilator-associated bacterial pneumonia caused by susceptible isolates of
Acinetobacter baumannii–calcoaceticus
in adults. A population pharmacokinetic (PK) model of sulbactam–durlobactam in plasma was developed using data from eight Phase 1–3 studies. A total of 432 subjects and 8,100 plasma concentrations were available for the population PK data set. The combined model was a four-compartment (two compartments per drug) model with linear kinetics. Both renal clearance and nonrenal clearance were estimated, and total clearance was calculated as the sum of renal and nonrenal clearance. Individual renal clearances were scaled by baseline creatinine clearance. The sampling–importance–resampling analysis indicated that the parameters were estimated reliably with adequate precision. Hemodialysis (HD) and epithelial lining fluid (ELF) sub-models were developed for each analyte separately. Intermittent HD resulted in an approximately 30% decrease in the daily area under the concentration–time curve (AUC
0–24
) when HD was started 1 hour after the end of the infusion. Assuming protein binding estimates of 10% and 38% for durlobactam and sulbactam, respectively, ELF penetration ratios were found to be 41.3% for durlobactam and 86.0% for sulbactam. Of the statistically significant covariates of PK identified, which included body weight, body mass index, infection type, and region of origin, renal function was the only clinically relevant covariate. Overall, a robust description of the plasma PK of sulbactam and durlobactam was achieved. The resultant population PK model was expected to be appropriate for model-based simulations and assessment of pharmacokinetic–pharmacodynamic relationships.
SARS-CoV-2 entry and fusion are independent of ACE2 localization to lipid rafts
Authors: Bolland William , Marechal Inès , Petiot Chloé , Porrot Françoise , Guivel-Benhassine Florence , Brelot Anne , Casartelli Nicoletta , Schwartz Olivier , Buchrieser Julian 
Published: November 2024

ABSTRACT

Membrane fusion occurs at the early stages of SARS-CoV-2 replication, during entry of the virus, and later during the formation of multinucleated cells called syncytia. Fusion is mediated by the binding of the viral Spike protein to its receptor ACE2. Lipid rafts are dynamic nanodomains enriched in cholesterol and sphingolipids. Rafts can act as platforms for entry of different viruses by localizing virus receptors, and attachment factors to the same membrane domains. Here, we first demonstrate that cholesterol depletion by methyl-beta-cyclodextrin inhibits Spike-mediated fusion and entry. To further study the role of ACE2 lipid raft localization in SARS-CoV-2 fusion and entry, we designed a GPI-anchored ACE2 construct. Both ACE2 and ACE2-GPI proteins were similarly expressed at the plasma membrane. Through membrane flotation assays, we show that in different cell lines, ACE2-GPI localizes predominantly to raft domains of the plasma membrane while ACE2 is non-raft associated. We then compare the ability of ACE2 and ACE2-GPI to permit SARS-CoV-2 entry, replication, and syncytia formation of different viral variants. We find little difference in the two proteins. Our results demonstrate that SARS-CoV-2 entry and fusion are cholesterol-dependent and raft-independent processes.

IMPORTANCE
Rafts are often exploited by viruses and used as platforms to enhance their entry into the cell or spread from cell to cell. The membrane localization of ACE2 and the role of lipid rafts in SARS-CoV-2 entry and cell-to-cell spread are poorly understood. The function of lipid rafts in viral fusion is often studied through their disruption by cholesterol-depleting agents. However, this process may have off-target impacts on viral fusion independently of lipid-raft disruption. Therefore, we created an ACE2 construct that localizes to lipid rafts using a GPI anchor. Conversely, wild-type ACE2 was non-raft associated. We find that the localization of ACE2 to lipid rafts does not modify the fusion dynamics of SARS-CoV-2.
Effect of metronidazole on concentrations of vaginal bacteria associated with risk of HIV acquisition
Authors: Valint D. J. , Fiedler Tina L. , Liu Congzhou , Srinivasan Sujatha , Fredricks David N. 
Published: November 2024

ABSTRACT

Several bacterial vaginosis (BV)-associated bacteria have been associated with elevated risk of human immunodeficiency virus (HIV) acquisition; however, susceptibility of these bacteria to antibiotics is poorly understood. Vaginal samples were collected from 22 persons daily for 2 weeks following BV diagnosis. Metronidazole treatment was prescribed for 5–7 days. Changes in bacterial concentrations were measured with taxon-specific 16S rRNA gene quantitative PCR (qPCR) assays. A culture-based antimicrobial assay confirmed presence of antibiotics in vaginal swab samples. Bacterial DNA concentrations decreased during antibiotic administration for all 13 bacterial taxa tested. Comparison of bacterial DNA concentrations in samples before administration of antibiotics to samples taken on the last day of antimicrobial assay-confirmed antibiotic presence showed a 2.25–4.78 log10-fold decrease across all taxa. Concentrations were frequently reduced to the qPCR assay’s limit of detection, suggesting eradication of bacteria. Mean clearance time varied across taxa (1.2–7.9 days), with several bacteria (e.g.,
Sneathia
spp., Vaginal TM7, and
Eggerthella
-like sp.) taking >7 days to suppress. Metronidazole reduces quantities of bacterial taxa associated with increased HIV acquisition risk.

IMPORTANCE
Human immunodeficiency virus (HIV) transmission through sex remains a major public health challenge despite efforts at risk reduction and use of anti-retroviral pre-exposure prophylaxis. Many bacterial vaginosis (BV)-associated vaginal bacteria have been associated with increased HIV infection risk among women. If these bacteria help mediate HIV infection risk, then eradication of these bacteria is one potential strategy to reduce this risk. However, the best approach to eradicate HIV-high risk bacteria from the vagina is not known. We analyzed vaginal swabs collected daily from women with BV to determine the impact of metronidazole treatment on 13 vaginal bacterial taxa linked to elevated risk of HIV infection through use of taxon-directed quantitative PCR assays. We conclude that eradication of high-risk vaginal bacteria using metronidazole is one promising avenue for reducing HIV acquisition risk, and we provide evidence that a 5–7-day treatment course may not be sufficient to suppress all bacteria.
Association of body index with fecal microbiome in children cohorts with ethnic–geographic factor interaction: accurately using a Bayesian zero-inflated negative binomial regression model
Authors: Huang Jian , Lu Yanzhuan , Tian Fengwei , Ni Yongqing 
Published: November 2024

ABSTRACT

The exponential growth of high-throughput sequencing (HTS) data on the microbial communities presents researchers with an unparalleled opportunity to delve deeper into the association of microorganisms with host phenotype. However, this growth also poses a challenge, as microbial data are complex, sparse, discrete, and prone to zero inflation. Herein, by utilizing 10 distinct counting models for analyzing simulated data, we proposed an innovative Bayesian zero-inflated negative binomial (ZINB) regression model that is capable of identifying differentially abundant taxa associated with distinctive host phenotypes and quantifying the effects of covariates on these taxa. Our proposed model exhibits excellent accuracy compared with conventional Hurdle and INLA models, especially in scenarios characterized by inflation and overdispersion. Moreover, we confirm that dispersion parameters significantly affect the accuracy of model results, with defects gradually alleviating as the number of analyzed samples increases. Subsequently applying our model to amplicon data in real multi-ethnic children cohort, we found that only a subset of taxa were identified as having zero inflation in real data, suggesting that the prevailing understanding and processing of microbial count data in most previous microbiome studies were overly dogmatic. In practice, our pipeline of integrating bacterial differential abundance in microbiome data and relevant covariates is effective and feasible. Taken together, our method is expected to be extended to the microbiota studies of various multi-cohort populations.

IMPORTANCE
The microbiome is closely associated with physical indicators of the body, such as height, weight, age and BMI, which can be used as measures of human health. Accurately identifying which taxa in the microbiome are closely related to indicators of physical development is valuable as microbial markers of regional child growth trajectory. Zero-inflated negative binomial (ZINB) model, a type of Bayesian generalized linear model, can be effectively modeled in complex biological systems. We present an innovative ZINB regression model that is capable of identifying differentially abundant taxa associated with distinctive host phenotypes and quantifying the effects of covariates on these taxa, and demonstrate that its accuracy is superior to traditional Hurdle and INLA models. Our pipeline of integrating bacterial differential abundance in microbiome data and relevant covariates is effective and feasible.
Mercury efficiently volatilized but not completely removed from sediments around igneous intrusions
Authors: Frieling Joost , Svensen Henrik H. , Mather Tamsin A. 
Published: November 2024

Abstract
The impact of large igneous province (LIP) volatile emissions from magmatic and thermogenic sources has been widely studied using sedimentary mercury (Hg). Igneous sill emplacement in sedimentary basins was a key component of several LIPs, generating environmentally significant amounts of thermogenic gases (e.g., CH4, CO2, Hg) and fluids by heating sedimentary rocks. Understanding these processes is key to realizing the promise of Hg in sedimentary archives to understand LIP impacts and track LIP carbon outputs. Published data show sedimentary rocks in contact aureoles of such LIP-related intrusions retain some Hg despite exposure temperatures above the stability limit of sedimentary Hg phases (&gt;300 °C). We examine this unexpected Hg retention using Hg thermal desorption profiles (TDPs). The TDPs reveal a remarkable pattern in Hg speciation related to intrusions: with increasing aureole temperature, Hg release is progressively dominated by a low-temperature phase that could have formed only during or after cooling of the intrusion. We calculate a temperature-dependent Hg volatilization efficiency and show that 70%–100% of Hg was mobilized above 350 °C. Despite the efficient volatilization, only ~50% of the volatilized Hg was removed from the studied aureole. Mercury recapture in and around the thermal aureole likely extends into the associated hydrothermal vent systems, reduces the Hg:C ratios of emitted thermogenic gases, and may lead to underestimation of Hg-based LIP thermogenic CO2 and CH4 emissions estimates and their environmental impact.
Endometriosis and uterine fibroids and risk of premature mortality: prospective cohort study
Authors: Wang Yi-Xin , Farland Leslie V , Gaskins Audrey J , Wang Siwen , Terry Kathryn L , Rexrode Kathryn M , Rich-Edwards Janet W , Tamimi Rulla , Chavarro Jorge E , Missmer Stacey A 
Published: November 2024

Abstract

Objective
To prospectively assess the effect of endometriosis and uterine fibroids on the long term risk of premature mortality (younger than 70 years).

Design
Prospective cohort study

Setting
The Nurses’ Health Study II, United States (1989-2019).

Participants
110 091 women aged 25-42 years in 1989 without a history of hysterectomy before endometriosis or fibroids diagnosis, cardiovascular diseases, or cancer.

Main outcome measures
Hazard ratios (estimated by Cox proportional hazards models) for total and cause specific premature mortality according to laparoscopically confirmed endometriosis or ultrasound or hysterectomy confirmed uterine fibroids reported in biennial questionnaires.

Results
4356 premature deaths were recorded during 2 994 354 person years of follow-up (27.2 years per person), including 1459 from cancer, 304 from cardiovascular diseases, and 90 from respiratory diseases. The crude incidence of all cause premature mortality for women with and without laparoscopically confirmed endometriosis was 2.01 and 1.40 per 1000 person years, respectively. In age adjusted models, laparoscopically confirmed endometriosis was associated with a hazard ratio of 1.19 (95% confidence interval 1.09 to 1.30) for premature death; these models were strengthened after also adjusting for potential confounders including behavioral factors (1.31, 1.20 to 1.44). Cause specific mortality analyses showed that the association was largely driven by mortality from senility and ill-defined diseases (1.80, 1.19 to 2.73), non-malignant respiratory diseases (1.95, 1.11 to 3.41), diseases of the nervous system and sense organs (2.50, 1.40 to 4.44), and malignant neoplasm of gynecological organs (2.76, 1.79 to 4.26). Ultrasound or hysterectomy confirmed uterine fibroids were not associated with all cause premature mortality (1.03, 0.95 to 1.11), but were associated with a greater risk of mortality from malignant neoplasm of gynecological organs (2.32, 1.59 to 3.40) in cause specific mortality analyses. The risk of mortality caused by cardiovascular and respiratory diseases varied according to joint categories of endometriosis and uterine fibroids, with an increased risk of all cause premature mortality among women reporting both endometriosis and uterine fibroids.

Conclusion
Women with a history of endometriosis and uterine fibroids might have an increased long term risk of premature mortality extending beyond their reproductive lifespan. These conditions were also associated with an increased risk of death due to gynecological cancers. Endometriosis was associated with a greater risk of non-cancer mortality. These findings highlight the importance for primary care providers to consider these gynecological disorders in their assessment of women's health.
Efficacy and safety of hyperbaric oxygen therapy for Parkinson’s disease with cognitive dysfunction: protocol for a systematic review and meta-analysis
Authors: Tan Weiqiang , Pan Zhaoquan , Xie Fengxi 
Published: November 2024

IntroductionThe presence of cognitive dysfunction notably affects the quality of life in individuals diagnosed with Parkinson’s disease (PD) and is often recognised as a non-motor symptom. Comprehensive studies have shown the possible advantages of hyperbaric oxygen therapy (HBOT) in alleviating cognitive deficits in these individuals. This systematic review aims to investigate the practicality of incorporating HBOT within a more extensive therapeutic framework for PD, with a specific focus on cognitive symptoms.Methods and analysisA comprehensive literature review will be conducted utilising various databases such as PubMed and Cochrane Library and so on. The duration of the search will encompass the entire timeline from the initiation of each database up to 1 April 2024. This investigation seeks to uncover randomised controlled trials that explore the efficacy and safety of HBOT in patients with PD who are facing cognitive impairments. The authors' autonomous screening and extraction of data will facilitate the attainment of impartial results. The assessment of possible biases will be conducted using the Cochrane risk-of-bias tool, while statistical analyses will be executed with RevMan V.5.3 and Stata V.15.0.Ethics and disseminationAs this review synthesises and evaluates previously conducted studies, the requirement for ethical approval is not applicable. The findings from this review will be shared via academic publications, comprehensive reports and presentations at pertinent conferences.PROSPERO registration numberCRD42024504763
Adolescents’ Daily Lives (ADL) project: an intensive longitudinal design study protocol examining the associations between physical literacy, movement behaviours, emotion regulation and mental health
Authors: Ames Megan , Srinivasa Gopalan Sharan , Sihoe C Emmett , Craig Stephanie G , Garcia-Barrera Mauricio , Liu Sam , Rhodes Ryan , Rush Jonathan , Buckler E Jean 
Published: November 2024

IntroductionAdolescence represents a critical developmental period, with changes in emotional regulation capacities influencing physical and mental health. With less than 6% of Canadian youth currently meeting the 24-hour movement guidelines for physical activity, sleep and sedentary behaviour, there is an urgent need to understand the potential association between movement behaviours, physical literacy, emotional regulation and mental health during adolescence. Additionally, there is a need to better understand these associations among equity-deserving groups. We developed the Adolescents’ Daily Lives (ADL) project to identify how, when, under what contexts and to whom to promote healthy engagement in movement behaviours to optimise youth mental health.Methods and analysisFor the ADL project, we will employ a 14-day intensive longitudinal design to investigate the associations between physical literacy, movement behaviours, emotion regulation and mental health among a diverse sample of 120 adolescents (ages 13–17 years) living in the Greater Victoria Area, British Columbia, Canada. A comprehensive baseline survey and movement competence test, assessing physical and mental well-being, 24-hour movement behaviours (ie, physical activity, sleep and sedentary behaviours) and physical literacy, will be accompanied by daily diary surveys and accelerometer-based movement tracking (ie, Fitbit Inspire 3) to assess daily fluctuations in movement behaviour, emotional regulation and mood. Multivariate analyses, including multilevel modelling, multilevel structural equation modelling and Bayesian hierarchical continuous-time SEM, will be used to model the repeated measures data and understand the simultaneous variations in daily movement behaviours, emotion regulation and mental health.Ethics and disseminationThe ADL project received ethical approval from the University of Victoria Behavioural Research Ethics Board (protocol #22-0262). Study participation is voluntary, and data collection will be anonymised to protect participant privacy and confidentiality. Research findings will be shared through academic publications and conference proceedings. Through knowledge mobilisation resources, cocreated with the youth community advisory board, relevant findings will be shared directly with the wider community of adolescents.