bluuebunny/crossref_metadata_2025_split huggingface dataset:
Свойства принципа конституционности деятельности субъектов правоотношений
Authors: Mosin Sergey 
Published: December 2024

В статье рассматриваются свойства принципа конституционности деятельности субъектов правоотношений, а именно: аксиоматичность, презумптивность и фиктивность. Целью данного исследования является подтверждение гипотезы о том, что конституционные принципы являются полноценными правовыми установлениями, которые наделены всеми необходимыми свойствами для достижения конституционных целей. Изучение свойств принципа конституционности деятельности субъектов правоотношений осуществлялось с помощью системно-структурного, сравнительно-правового, формально-правового и других методов. Использование этих методов в их сочетании предопределило обращение не только к актуальным проблемам конституционного права, но и к вопросам теории права, а также других отраслей права, что позволило наиболее объективно и комплексно подойти к изучению свойств конституционных принципов. Исследование свойств принципа конституционности деятельности субъектов правоотношений основано на том, что, формулируя базовое правило и определяя направления развития правовой системы, конституционный принцип тем самым обладает свойством аксиоматичности. Вместе с тем конституционный принцип обладает свойством презумптивности вследствие обязанности правоприменителя исходить из предположения о соблюдении положений такого принципа всеми субъектами правоотношений, обеспечивая тем самым необходимую стабильность правовой системы государства. При этом для достижения полноценной стабильности правовой системы одновременно с предположением о соблюдении субъектами правоотношений положений конституционных принципов должна предусматриваться возможность контроля за таким соблюдением. Решением становится применение правовых презумпций. В связи с этим в рамках свойства презумптивности конституционных принципов конституционные презумпции неразрывно взаимосвязаны с корреспондирующими им конституционными принципами. В свою очередь, обладая свойством фиктивности, конституционный принцип позволяет гибко интерпретировать составляющие такой конституционный принцип положения и, как следствие, создавать необходимую нормативную правовую базу. Таким образом, принцип конституционности деятельности субъектов правоотношений, обладая вышеуказанными свойствами, в полном объеме определяет общеобязательные основные правила и направления развития правовой системы, обеспечивает ее стабильность и возможность своевременного развития.Для цитирования: Мосин С.А. Свойства принципа конституционности деятельности субъектов правоотношений // Право. Журнал Высшей школы экономики. 2022. Т. 15. № 2. С. 105–127. DOI:10.17323/2072-8166.2022.2.105.127.
Значение фундаментальных свобод ЕС для налогового права ФРГ и ЕС
Authors: Shteber Mikhael' 
Published: December 2024

Директивой ЕС о системе НДС в значительной степени была унифицирована система законодательств стран-членов ЕС в отношении налога с оборота, относящегося к подгруппе так называемых непрямых налогов. В то же время в отношении так называемых прямых налогов, к которым относятся налог на доходы физических лиц, налог на доходы юридических лиц, а также промысловый налог, европейским законодательством не было установлено четких критериев правового характера, на основании которых была бы возможна унификация данной подгруппы налогов. В отношении данной подгруппы прямых налогов возможна лишь частичная унификация, посредством которой могут быть унифицированы лишь отдельные налоговые положения стран-членов ЕС. Подобного рода частичная унификация оставляет за странами-членами ЕС право внесения необходимых законодательных изменений в отношении прямых налогов без учета фискальных интересов других стран-членов ЕС, равно как и ЕС в целом. Несмотря на это, наличие подобного рода частичной унификации не может быть расценено в качестве юридического обстоятельства, которым можно было бы оправдать существенное ограничение прав и свобод граждан стран-членов ЕС в результате введения рестриктивных норм налогового характера, ибо, согласно прецедентному праву Европейского Суда, для законодательств стран-членов ЕС в отношении прямых налогов ориентиром должны служить фундаментальные свободы ЕС, зафиксированные в Договоре о функционировании ЕС. Так, в частности, Европейским Судом были выявлены многочисленные факты нарушения нормами национальных налоговых законодательств фундаментальных свобод ЕС, которые впоследствии были устранены странами-членами ЕС посредством внесения соответствующих изменений в целях адаптации национальных законодательств стран-членов ЕС под требования европейского права. Посредством разбора знаковых решений Европейского Суда в данной статье анализируется влияние фундаментальных свобод ЕС и прецедентного права Европейского Суда на процесс непрямой унификации законодательств стран-членов ЕС в отношении прямых налогов.Для цитирования: Штебер М. Значение фундаментальных свобод ЕС для налогового права ФРГ и ЕС // Право. Журнал Высшей школы экономики. 2022. Т. 15. № 2. C. 216– 243. DOI:10.17323/2072-8166.2022.2.216.243
Family Resilience Assessment Scale (FRAS): avaliando sua estrutura interna no contexto brasileiro
Authors: Santos de Oliveira Lays Brunnyeli , Nunes da Fonseca Patrícia , Nascimento da Silva Paulo Gregório , Guimarães Amorim Luize Anny 
Published: December 2024

Objetivou-se reunir evidências psicométricas da Family Resilience Assessment Scale (FRAS). Foram realizados dois estudos com participantes de diferentes estados brasileiros. No primeiro (n = 314), a maioria foi do sexo feminino (56,4%), sendo realizada uma análise fatorial exploratória, que sugeriu uma estrutura tetrafatorial, com adequada precisão. No segundo (n = 336), maioria do sexo feminino, submetidos à hemodiálise (46,4%). A análise fatorial confirmatória corroborou a estrutura tetrafatorial (comunicação familiar e resolução de problemas, manter uma perspectiva positiva, utilização de recursos sociais e econômicos e, espiritualidade familiar), por meio de uma versão reduzida da FRAS. Em suma, os resultados mostraram que a versão reduzida da FRAS pode ser uma alternativa para avaliar a resiliência familiar e seus correlatos.
Extracellular vesicles in liquid biopsies: there is hope for oral squamous cell carcinoma
Authors: Leung Leanne Lee , Qu Xinyu , Chen Bojie , Chan Jason YK. 
Published: December 2024

Current approaches to oral cancer diagnosis primarily involve physical examination, tissue biopsy, and advanced computer-aided imaging techniques. However, despite these advances, patient survival rates have not significantly improved. Hence, there is a critical need to develop minimally invasive tools with high sensitivity and specificity to improve patient survival and quality of life. Liquid biopsy is a non-invasive, real-time method for predicting cancer status and potentially serves as a biomarker source for treatment response. Liquid biopsy includes rich biologically relevant components, such as circulating tumor cells, circulating tumor DNA, and extracellular vesicles (EVs). EVs are particularly intriguing due to their relatively high abundance in most biofluids, with the potential to identify specific cargo derived from circulating tumor EVs. Moreover, normal cells in lymph nodes can uptake EVs, fostering a pre-metastatic microenvironment that facilitates lymph node metastases - a common occurrence in oral cancers.
This review encompasses English language publications over the last twenty years, focusing on methods for isolating EVs from saliva, blood, and lymphatic fluids, as well as the collection methods employed. Seventeen cases met the inclusion criteria according to ISEV guidelines, including 10 saliva cases, 6 blood cases, and 1 lymphatic fluid case. This review also highlighted research gaps in oral squamous cell carcinoma (OSCC) EVs, including a lack of multi-omics studies and the exploration of potential EV markers for drug resistance, as well as a notable underutilization of microfluidic technologies to translate liquid biopsy EV findings into clinical applications.
Bacillus proteolyticus UPMC1508: A novel bacterial strain capable of biologically synthesize iron oxide nanoparticles
Authors: Hasan Yusur Ramzi , Wong Fadzlie Wong Faizal , Halim Murni , Ashari Siti Efliza , Mohamad Rosfarizan 
Published: December 2024

Abstract

Antibiotic resistance is a growing concern due to the overuse of antibiotics. Alternative treatments, such as nanoparticles, are being explored. Biological synthesis of iron oxide nanoparticles (Fe3O4-NPs) via probiotics offers a sustainable and cost-effective method over the toxic chemical approaches, but there are challenges regarding its heavy metal resistance and the toxicity of the obtained nanoparticles. Thus, this research aims to biologically synthesize Fe₃O₄-NPs via a new bacterial isolate and evaluate its toxicity. The objectives are to isolate and characterize a novel bacterial isolate with probiotic potential. Then, to biologically synthesize and characterize Fe3O4-NPs via Transmission Electron Microscopy (TEM), Field Emission Scanning Electron Microscopy (FE-SEM), Powder Diffraction Techniques (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), and nanosizer. Finally, to evaluate its cytotoxicity potential via MTT– (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. A total of 12 samples were collected from six different sites in Selangor, Malaysia. The 16s rRNA sequencing came closest to Bacillus proteolyticus UPMC1508 (99.87%). This strain has lower antibiotic resistance and high Fe-tolerance (MTC = 0.8 mg.mL− 1). It successfully synthesized Fe3O4-NPs, which exhibited absorption curves between 290–300 nm. TEM and FE-SEM indicated spherical formed Fe3O4-NPs; the average diameter was 5.12 ± 0.95 nm. Meanwhile, XRD peaks revealed that the grain size was around 32.61 nm. The nanosizer revealed a hydrodynamic diameter of around 104 nm with a good Polydispersity index (PDI) value (0.217). FT-IR indicated a satisfactory stability of Fe3O4-NPs after 2 months. Finally, Fe3O4-NPs showed low toxicity at 0.031 mg.mL− 1.The findings revealed that the novel isolated B. proteolyticus UPMC1508 has high Fe-heavy metal tolerance and less antibiotic resistance. Furthermore, it successfully synthesized Fe3O4-NPs with satisfactory stability and safety, making them suitable for therapeutic platforms, such as antibacterial and anticancer. The significance of this study lies in offering an eco-friendly, low-cost synthesis approach while expanding the applicability of safe Fe3O4-NPs for biomedical applications.
Synergistic effect of Ce doping and phosphorylation on optimizing the low temperature NH 3 -SCR activity of the spent V 2 O 5 -WO 3 /TiO 2 catalyst
Authors: Wang Qiulin , Qi Xiongfei , Wang Haonan , Tang Minghui , Lu Shengyong , Liu Dunyu , Jin Jing 
Published: December 2024

Abstract

The reactivation and subsequent reuse of the exhausted SCR catalyst holds significant economic and environmental advantages. Water and acid washing along with thermal regeneration were commonly utilized to eliminate toxic substances from the catalyst surface, while these processes often resulted in the undesired loss of active components. In this research, an impregnation approach was adopted to replenish the depleted active constituents of the spent V2O5-WO3/TiO2 catalyst (denoted as VW/Ti). Experimental investigations were conducted to evaluate the influence of Ce/P doping on both the Selective Catalytic Reduction (SCR) activity of this rejuvenated catalyst and its resistance to the detrimental effects of SO2/H2O exposure. The optimal performance is achieved with the Ce/P co-doped VW/Ti catalyst, demonstrating a remarkable efficiency in removing nearly 100% of NOx across a broad temperature ranging from 150°C to 450°C. The incorporation of a proper content of Ce serves to elevate the VOx surface density from a sub-monolayer state to a monolayer and foster the formation of more surface oxygen vacancy. This transformation positively contributes to enhancing both the catalytic activity and the resistance to the SO2/H2O poisoning. Further treatment with H3PO4, although rendering the catalyst more susceptible to H2O, achieves a delicate balance between its surface acidity and redox capabilities. This optimized state not only enhances the catalyst's robustness but also further widens the active temperature window.
RLB-MALBAC: A rapid, non-transposase method for synthesizing unbiased sequencing libraries from low amount of genomic DNA for Nanopore sequencing
Authors: Gadkar Vijay J. , Goldfarb David M. , Dhaliwal Sukh , Gubbay Jonathan B. , Tilley Peter A. G. 
Published: December 2024

Abstract

Background Successful sequencing of genomes on Oxford Nanopore Technologies (ONT) sequencing platforms is dependant on obtaining sufficient amounts of high-quality genomic DNA. ONT’s Rapid PCR kit (SQK-RPB114.24) kit is specifically designed to address input template limitations, whereby low amounts of target DNA (1–5 ng) is pre-amplified to > 100–200 ng using PCR. The success of this workflow is however dependent on the quality of input DNA, which as per the SQK-RPB114.24 kit’s instructions, should be of length > 4kb for an efficient tagmentation - a step pre-cursor to PCR amplification. Obtaining high quality, intact genomic DNA from clinical samples or viral genomes, can sometimes be challenging and this method seeks to address this limitation.Methods In this study, a novel non-tagmentation based method is presented to prepare sequencing libraries from low amounts of genomic DNA (< 5-100 ng). Our method, based on a quasilinear amplification method, the Multiple Annealing and Looping Based Amplification Cycles chemistry (MALBAC), to randomly introduces a 21-nucleotide, PCR anchor (“RLB tag”), unbiasedly across the target. This method, henceforth referred to as RLB-MALBAC, results in the generation of RLB-tagged genomic libraries which are then compatible with the PCR dependent RLB-BC0-24 series barcodes of the SQK-RPB114.24 kit.Results The RLB-MALBAC method was used to sequence genomes from Escherichia coli bacteriophage lambda (48.5kb) virus as well as clinically relevant bacteria (2–5 Mb genome range). To demonstrate one potential application of RLB-MALBAC, we tested the generated contigs for the presence of antimicrobial resistance (AMR) genes of the β-lactamase class. Using the Comprehensive Antibiotic Resistance Database (CARD) prediction tool, we found a 100% concordance between the β-lactamase genes predicted by RLB-MALBAC and the reference sequences (Illumina + Nanopore hybrid assemblies) for ATCC bacterial isolates. We also predicted resistance phenotypes from clinical bacterial isolates confirmed to carry β-lactamases.Discussion As the SQK-RPB114.24 kit is PCR dependent, a short read-length (2-3kb) is expected using the RLB-MALBAC method, which is a major limitation of this workflow. The versatility and simplicity of the RLB-MALBAC method should find broad application in other areas of microbiology, for e.g., plasmid, viral and single cell genomics, where there may be need to amplify from limited amounts of genomic material.
Comprehensive geological analysis and evaluation of the feasibility of renovating the Wanshunchang gas reservoir into a gas storage facility
Authors: Tong Zebin , Hu Zhonggui , Qiu Longwei , Zhang Na , Zhu Huayin , Zhou Daoyong , Zhou Gang , Chen Xiao , Shao Yanlin , Hu Jiuzhen 
Published: December 2024

Abstract

As many oil and gas reservoirs approach depletion stages in the future, alongside growing energy storage demands, constructing gas storage facilities becomes critical for ensuring a stable natural gas supply. Consequently, a comprehensive geological analysis is essential to evaluate the feasibility of converting depleted gas reservoirs into gas storage facilities. The W gas reservoir in the Sichuan Basin, China, is nearing depletion and presents potential for conversion into a gas storage facility. This study provides a comprehensive geological evaluation to determine its feasibility for such conversion. Exploration and production data indicate that the W gas reservoir is a pore-fracture type carbonate reservoir. Vertically, the sub-layer beneath C2hl2 demonstrates the best physical properties. Horizontally, the area enclosed by wells C30, C10, and C18 exhibits favorable reservoir characteristics. The reservoir's caprock comprises a superimposed composite system, including the direct caprock of the Liangshan Formation and an overlying ultra-thick dense rock layer, which is extensive and exhibits high breakthrough pressure. Nine major faults control the structural trap's shape and scale. These faults, in a compressive state, are well-sealed due to fault gouge, enhancing their closure. The reservoir space is primarily pore-fracture type, and rapid injection and withdrawal induce minimal pressure changes, indicating low stress sensitivity. The comprehensive evaluation concludes that the Carboniferous gas reservoir possesses favorable geological conditions, making it suitable for conversion into a gas storage facility. This study offers a preliminary geological evaluation of the feasibility of converting fault-controlled fracture-type carbonate reservoirs into gas storage facilities and outlines directions for future research.
Agricultural practices and environmental factors drive microbial communities in the mezcal-producing Agave angustifolia Haw.
Authors: Contreras-Negrete Gonzalo , Valiente-Banuet Alfonso , Partida-Martínez Laila P. , Hernández-López Antonio 
Published: December 2024

Abstract

Mezcal, a traditional Mexican alcoholic beverage, has been a vital source of livelihood for indigenous and rural communities for centuries. However, increasing international demand is exerting pressure on natural resources and encouraging intensive agricultural practices. This study investigates the impact of management practices (wild, traditional, and conventional) and environmental factors on the microbial communities associated with Agave angustifolia, a key species in mezcal production. High-throughput sequencing of the 16S rRNA and ITS2 gene regions revealed distinct prokaryotic and fungal community structures across different plant compartments (endosphere, episphere, and soil), identifying 8,214 prokaryotic and 7,459 fungal ASVs. Core microbial communities were dominated by Proteobacteria, Actinobacteria, Ascomycota, and Basidiomycota. Alpha diversity analyses showed significant increases in prokaryotic diversity from the endosphere to soil, while fungal diversity remained stable. Notably, conventional management practices were associated with reductions in beneficial microbial taxa. Environmental factors such as precipitation and temperature significantly influenced microbial diversity and composition, especially in the rhizosphere. Beta diversity patterns underscored the strong impact of plant compartment, with management practices and aridity further shaping microbial communities. These results reveal the intricate interactions between management practices, environmental conditions, and microbial diversity, providing valuable insights for the sustainable cultivation of A. angustifolia.
NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein.
Authors: Hirakawa Tomoaki , Taniuchi Megumi , Iguchi Yoko , Bogahawaththa Sudarma , Yoshitake Kiko , Werellagama Shanika , Uemura Takeshi , Tsujita Tadayuki 
Published: December 2024

Abstract

Polyamines (putrescine, spermidine, and spermine) are among the most abundant intracellular small molecular metabolites, with concentrations at the mM level. The ratios of these three molecules remain constant under physiological conditions. Stress (i.e. polyamine overload, oxidative stress, aging, etc.) triggers the catabolic conversion of spermine to spermidine, ultimately yielding acrolein and hydrogen peroxide. The potential of acrolein to induce DNA damage and protein denaturation is 1,000 times greater than that of reactive oxygen species. We have shown that these polyamine metabolic pathways also involve the nuclear factor erythroid–2–related factor 1 (NRF1) transcription factor. In our chemically-inducible, liver-specific Nrf1-knockout mice, the polyamine catabolic pathway dominated the anabolic pathway, producing free acrolein and accumulating acrolein-conjugated proteins in vivo. This metabolic feature implicates SMOX as an important causative enzyme. Chromatin immunoprecipitation and reporter assays confirmed that NRF1 directly suppressed Smox expression. This effect was also observed in vitro. Ectopic overexpression of SMOX increased the accumulation of free acrolein and acrolein-conjugated proteins. SMOX knockdown reversed the accumulation of free acrolein and acrolein-conjugated proteins. Our results show that NRF1 typically suppresses Smox expression when NRF1 is downregulated, SMOX is upregulated, and polyamine metabolic pathways are altered, producing low molecular weight polyamines and acrolein.
Research on the hydrodynamic characteristics of pumpjet vector propulsor submersible yaw motion
Authors: Xue Tongqing , Han Wei , Li Rennian , Zhang Sicong , Chen Ran , Wang Qifan 
Published: December 2024

Abstract

The potential value of the new type of vector propulsor in submarine movement has been confirmed. However, some key mechanical issues are not fully understood, especially the hydrodynamic characteristics during oblique motion. By using dynamic mesh simulation methods, a systematic study was conducted on the fluid dynamics behavior of pump jet vector propulsor submarines during oblique and yawing processes, supplemented by the scientific validity of related experimental verification results. The research indicates that oblique movement causes a local stagnation positive pressure zone to form at the bow of the hull and a relative back pressure zone in the middle of the pump shell. As the angle of drift during oblique movement increases, significant improvements are observed in the lateral force, lateral velocity, and lateral moment of the submarine. During yawing motion, a negative pressure zone appears on the right side of the bow, with a local positive pressure zone appearing on the left side. In both oblique and yawing movements, the rotational speed has an amplifying effect on the appearance of the jet wake phenomenon for the submarine. Based on numerical results, a polynomial fitting method is used to establish a mathematical model for the variation of the speed coefficient and angular velocity system of the pump jet vector propulsor submarine with the spiral mixed-flow pump speed. This study provides theoretical guidance for the application and optimization of pump jet vector propulsors.
Nondestructive testing of Aircraft Composite Skin Defects based on Electromagnetic Sensor
Authors: zhaoxuan zhu , kaiyu qin 
Published: December 2024

Abstract

The detection of aircraft skin is an indispensable part of the design and µanufacturing process. This paper proposes a non-destructive testing µethod for aircraft coµposite skin defects based on electroµagnetic sensor. Firstly, Using the perturbation theory, an electroµagnetic nondestructive testing µodel of aircraft skin defects is established. Then, according to the plasµon theory, a nested coµpleµentary split resonant ring (CSRR) electroµagnetic sensor is designed. Siµulation using HFSS, it shows that it can effectively detect air hole defects with diaµeters of 2µµ and above and burial depths within 1µµ, fiber break defects with lengths above 4µµ, widths of 0.2µµ and above, and burial depths within 1µµ. Finally, a physical test was carried out, and a 10µµ diaµeter air hole defect in the aircraft skin saµple could be detected.Index Terms—Nondestructive Testing, Carbon Fiber Reinforced Plastic, Metamaterial Sensor