bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outpacing the pneumococcus: Antibody dynamics in the first few days following pneumococcal capsular antigen stimulation
Authors: Kimaro Mlacha Sheila Z. , Warira Anne , Gatakaa Hellen , Goldblatt David , Scott J. Anthony G. 
Published: October 2018

AbstractChildren in developing countries are frequently exposed to the pneumococcus, but few develop invasive pneumococcal disease (IPD). We test the hypothesis that natural variation exists in the rapidity of IgG responses following exposure to pneumococcal polysaccharides, and that these differences are sufficiently great to affect susceptibility to and outcome of IPD. We recruited children aged 24–36 months, who had recovered from IPD, and age-matched healthy controls and vaccinated them with 1 dose of the 23-valent PPV to mimic natural exposure. We collected serum samples after vaccination and analysed the dynamics of anti-polysaccharide antibody responses to several capsular antigens. Mean IgG response times to different serotypes were 6.4–7.3 days, with standard deviations of 0.9–1.85 days, suggesting a natural range in response times of up to 7 days. Serotype 1 elicited the largest fold-rise, serotype 23F the smallest. The proportion of responses achieved by day 7 was similar in children with a history of IPD and healthy children. There was considerable natural variation in the rapidity of anti-capsular IgG responses extending over 4–7 days. There was no evidence to suggest that children who have experienced IPD respond more slowly to heterologous pneumococcal capsular antigens than do healthy children.
The dynamic transcriptome and metabolomics profiling in Verticillium dahliae inoculated Arabidopsis thaliana
Authors: Su Xiaofeng , Lu Guoqing , Guo Huiming , Zhang Kaixuan , Li Xiaokang , Cheng Hongmei 
Published: October 2018

AbstractVerticillium wilt caused by the soil-borne fungus Verticillium dahliae is a common, devastating plant vascular disease notorious for causing economic losses. Despite considerable research on plant resistance genes, there has been little progress in modeling the effects of this fungus owing to its complicated pathogenesis. Here, we analyzed the transcriptional and metabolic responses of Arabidopsis thaliana to V. dahliae inoculation by Illumina-based RNA sequencing (RNA-seq) and nuclear magnetic resonance (NMR) spectroscopy. We identified 13,916 differentially expressed genes (DEGs) in infected compared with mock-treated plants. Gene ontology analysis yielded 11,055 annotated DEGs, including 2,308 for response to stress and 2,234 for response to abiotic or biotic stimulus. Pathway classification revealed involvement of the metabolic, biosynthesis of secondary metabolites, plant–pathogen interaction, and plant hormone signal transduction pathways. In addition, 401 transcription factors, mainly in the MYB, bHLH, AP2-EREBP, NAC, and WRKY families, were up- or downregulated. NMR analysis found decreased tyrosine, asparagine, glutamate, glutamine, and arginine and increased alanine and threonine levels following inoculation, along with a significant increase in the glucosinolate sinigrin and a decrease in the flavonoid quercetin glycoside. Our data reveal corresponding changes in the global transcriptomic and metabolic profiles that provide insights into the complex gene-regulatory networks mediating the plant’s response to V. dahliae infection.
Global genetic diversity of var2csa in Plasmodium falciparum with implications for malaria in pregnancy and vaccine development
Authors: Benavente Ernest Diez , Oresegun Damilola R. , de Sessions Paola Florez , Walker Eloise M. , Roper Cally , Dombrowski Jamille G. , de Souza Rodrigo M. , Marinho Claudio R. F. , Sutherland Colin J. , Hibberd Martin L. , Mohareb Fady , Baker David A. , Clark Taane G. , Campino Susana 
Published: October 2018

AbstractMalaria infection during pregnancy, caused by the sequestering of Plasmodium falciparum parasites in the placenta, leads to high infant mortality and maternal morbidity. The parasite-placenta adherence mechanism is mediated by the VAR2CSA protein, a target for natural occurring immunity. Currently, vaccine development is based on its ID1-DBL2Xb domain however little is known about the global genetic diversity of the encoding var2csa gene, which could influence vaccine efficacy. In a comprehensive analysis of the var2csa gene in >2,000 P. falciparum field isolates across 23 countries, we found that var2csa is duplicated in high prevalence (>25%), African and Oceanian populations harbour a much higher diversity than other regions, and that insertions/deletions are abundant leading to an underestimation of the diversity of the locus. Further, ID1-DBL2Xb haplotypes associated with adverse birth outcomes are present globally, and African-specific haplotypes exist, which should be incorporated into vaccine design.
A new approach based on targeted pooled DNA sequencing identifies novel mutations in patients with Inherited Retinal Dystrophies
Authors: Ezquerra-Inchausti Maitane , Anasagasti Ander , Barandika Olatz , Garay-Aramburu Gonzaga , Galdós Marta , López de Munain Adolfo , Irigoyen Cristina , Ruiz-Ederra Javier 
Published: October 2018

AbstractInherited retinal diseases (IRD) are a heterogeneous group of diseases that mainly affect the retina; more than 250 genes have been linked to the disease and more than 20 different clinical phenotypes have been described. This heterogeneity both at the clinical and genetic levels complicates the identification of causative mutations. Therefore, a detailed genetic characterization is important for genetic counselling and decisions regarding treatment. In this study, we developed a method consisting on pooled targeted next generation sequencing (NGS) that we applied to 316 eye disease related genes, followed by High Resolution Melting and copy number variation analysis. DNA from 115 unrelated test samples was pooled and samples with known mutations were used as positive controls to assess the sensitivity of our approach. Causal mutations for IRDs were found in 36 patients achieving a detection rate of 31.3%. Overall, 49 likely causative mutations were identified in characterized patients, 14 of which were first described in this study (28.6%). Our study shows that this new approach is a cost-effective tool for detection of causative mutations in patients with inherited retinopathies.
Association of night-waking and inattention/hyperactivity symptoms trajectories in preschool-aged children
Authors: Reynaud Eve , Forhan Anne , Heude Barbara , Charles Marie-Aline , Plancoulaine Sabine 
Published: October 2018

AbstractThe objective was to study the longitudinal associations between inattention/hyperactivity symptoms and night-waking in preschool-years, in light of their joint evolution. Within the French birth-cohort study EDEN, repeated measures of 1342 children’s night-waking and inattention/hyperactivity symptoms were collected at age 2, 3 and 5–6 through questionnaires. Trajectories were computed using group-based modeling. Logistic regressions, adjusted for confounding factors, were used to measure the association between trajectories and to determine risk factors for belonging to the identified joint trajectories. Two night-waking trajectories were observed, 20% of the children had a trajectory of “common night-waking”, and 80% a trajectory of “rare night-waking”. The children were distributed in three inattention/hyperactivity trajectories, a low (47%), medium (40%) and high one (13%). Both night-waking and inattention/hyperactivity trajectories showed persistence of difficulties in preschool years. The risk of presenting a high inattention/hyperactivity trajectory compared to a low one was of 4.19[2.68–6.53] for common night-wakers, compared to rare night-wakers. Factors associated with joint trajectories were parent’s education level and history of childhood behavioral problems, and the child’s gender, night-sleep duration and collective care at 2 years of age. Results suggest that children presenting behavioral difficulties would benefit from a systematic investigation of their sleep quality and conversely.
Effects of Precipitates Evolution on Low Stress Creep Properties in P92 Heat-resistant Steel
Authors: Han Hongguang , Shen Junjie , Xie Jiaxing 
Published: October 2018

AbstractThe evolution of precipitates in P92 heat-resistant steel sustained under thermal aging at 923 K for different times were analyzed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), while the effects of precipitates evolution on the low stress creep properties were experimentally analyzed through uniaxial creep testing and multiaxial “helicoid spring creep testing”. The results demonstrate that the coarsening of M23C6 carbide is significantly slower than the Laves phase in the thermal aging of 0~8000 h. The creep resistance of the P92 heat-resistant steel is enhanced at 923 K aging for 3000 h, and the strengthening effect is significantly apparent under the lower stresses. Moreover, the microstructure degradation factor of the P92 heat-resistant steel in the low stress region at 923 K is different that under the lower stresses of 20 MPa and 35 MPa is mainly Laves phase and the higher stresses of 65 MPa and 75 MPa is mainly M23C6 carbide.
Obese subcutaneous adipose tissue impairs human myogenesis, particularly in old skeletal muscle, via resistin-mediated activation of NFκB
Authors: O’Leary Mary F. , Wallace Graham R. , Davis Edward T. , Murphy David P. , Nicholson Thomas , Bennett Andrew J. , Tsintzas Kostas , Jones Simon W. 
Published: October 2018

AbstractAdiposity and adipokines are implicated in the loss of skeletal muscle mass with age and in several chronic disease states. The aim of this study was to determine the effects of human obese and lean subcutaneous adipose tissue secretome on myogenesis and metabolism in skeletal muscle cells derived from both young (18–30 yr) and elderly (>65 yr) individuals. Obese subcutaneous adipose tissue secretome impaired the myogenesis of old myoblasts but not young myoblasts. Resistin was prolifically secreted by obese subcutaneous adipose tissue and impaired myotube thickness and nuclear fusion by activation of the classical NFκB pathway. Depletion of resistin from obese adipose tissue secretome restored myogenesis. Inhibition of the classical NFκB pathway protected myoblasts from the detrimental effect of resistin on myogenesis. Resistin also promoted intramyocellular lipid accumulation in myotubes and altered myotube metabolism by enhancing fatty acid oxidation and increasing myotube respiration and ATP production. In conclusion, resistin derived from human obese subcutaneous adipose tissue impairs myogenesis of human skeletal muscle, particularly older muscle, and alters muscle metabolism in developing myotubes. These findings may have important implications for the maintenance of muscle mass in older people with chronic inflammatory conditions, or older people who are obese or overweight.
Karst Detection Beneath the Pyramid of El Castillo, Chichen Itza, Mexico, by Non-Invasive ERT-3D Methods
Authors: Chávez René E. , Tejero-Andrade Andrés , Cifuentes Gerardo , Argote-Espino Denisse L. , Hernández-Quintero Esteban 
Published: October 2018

AbstractCurrently, archaeologists perform excavations determined by previous geophysical studies to accurately establish the prospective targets and minimize site disturbance. Among others, one of the methods most widely employed is the Electrical Resistivity Tomography (ERT-2D, -3D). However, investigation of the subsoil of archaeological buildings is not possible to carry out with traditional geophysical methods, because the structure itself prevents it. Therefore, it is necessary to design non-invasive special arrays capable of characterizing the subsoil of such buildings, while preserving their historical context. Here we show how this procedure combined with sequences of resistivity observations at depth allowed us to detect a low resistivity body beneath the pyramid ofEl Castilloin Chichen Itza (Mexico). This feature may be associated with a cavity (karst) partially filled with sweet water. On the other hand, a natural cavity was discovered underEl Osariopyramid (south of El Castillo), at the end of the 19th century. Therefore, this pyramid was also studied to validate the effectiveness of this methodology, obtaining outstanding results. This method provides an interesting procedure to investigate the subsoil of archaeological structures for unveiling evidences that allow specialists to understand the religious meaning of these temples.
Reduced glucocerebrosidase activity in monocytes from patients with Parkinson’s disease
Authors: Atashrazm Farzaneh , Hammond Deborah , Perera Gayathri , Dobson-Stone Carol , Mueller Nicole , Pickford Russell , Kim Woojin Scott , Kwok John B. , Lewis Simon J. G. , Halliday Glenda M. , Dzamko Nicolas 
Published: October 2018

AbstractMissense mutations in glucocerebrosidase (GBA1) that impair the activity of the encoded lysosomal lipid metabolism enzyme (GCase) are linked to an increased risk of Parkinson’s disease. However, reduced GCase activity is also found in brain tissue from Parkinson’s disease patients withoutGBA1mutations, implicating GCase dysfunction in the more common idiopathic form of Parkinson’s disease. GCase is very highly expressed in monocytes, and thus we measured GCase activity in blood samples from recently diagnosed Parkinson’s disease patients. Flow cytometry and immunoblotting assays were used to measure levels of GCase activity and protein in monocytes and lymphocytes from patients with Parkinson’s disease (n = 48) and matched controls (n = 44). Gene sequencing was performed to screen participants forGBA1missense mutations. In the Parkinson’s disease patients, GCase activity was significantly reduced in monocytes, but not lymphocytes, compared to controls, even whenGBA1mutation carriers were excluded. Monocyte GCase activity correlated with plasma ceramide levels in the Parkinson’s disease patients. Our results add to evidence for GCase dysfunction in idiopathic Parkinson’s disease and warrant further work to determine if monocyte GCase activity associates with Parkinson’s disease progression.
Abnormally expressed miR-23b in Chinese Mongolian at high cardiovascular risk may contribute to monocyte/macrophage inflammatory reaction in atherosclerosis
Authors: He Li-ping , Zhao Xing-sheng , He Le-ping 
Published: October 2018

Background: The prevalence of coronary heart disease (CHD) appears to be high among Chinese Mongolians. MiR-23b has been proven to play a key role in atherosclerosis. The expression and role of miR-23b in the Mongolians at high cardiovascular risk were explored in the present study.
Methods: Forty cases of blood samples from the Mongolians at high cardiovascular risk were enrolled in the present study. The expression of miR-23b was quantified by quantitative real-time PCR. To induce monocytes differentiation into macrophages, HP-1 cells were cultured with phorbol 12-myristate 13-acetate. The level of inflammatory markers was determined by the enzyme-linked immunosorbent assay. The interaction between miR-23b and A20 was explored by the dual luciferase reporter assay.
Results: The expression of miR-23b in the Mongolian at high cardiovascular risk was higher than that in healthy Mongolian volunteers. Decrease in ATP-binding cassette transporter A1 caused by miR-23b is responsible for TC accumulation in the Mongolian at high cardiovascular risk. MiR-23b enhanced the oxidized low-density lipoprotein (oxLDL)-induced inflammatory response of THP-1 derived macrophage. MiR-23b regulated nuclear factor-κB (NF-κB) pathway through targeting A20. MiR-23b mediated oxLDL-induced inflammatory response of peripheral blood mononuclear cell in the Mongolian at high cardiovascular risk.
Conclusion MiR-23b enhanced oxLDL-induced inflammatory response of macrophages in the Mongolian at high cardiovascular risk through the A20/NF-κB signaling pathway, and thus contributing to atherosclerosis.
Comparative Efficacy of Alendronate upon Vertebral Bone Mineral Density and Fracture Rates in East Asians Versus Non-East Asians with Postmenopausal Osteoporosis: A Systematic Review and Meta-Analysis
Authors: Wang Yexin , Jia Gongwei , Song Jin , Kong Xiangqing , Zhang Weihong , Meng Chunyang 
Published: October 2018

AbstractBisphosphonates, such as alendronate, have become the most widely used and effective anti-resorptive therapy for postmenopausal osteoporosis. Previous genetic studies suggest that ethnicity may drive differing responses to bisphosphonate therapy in East Asians and non-East Asians. Therefore, the aim of this study was to comparatively evaluate the efficacy of alendronate upon lumbar spinal BMD and vertebral fracture rates in East Asians and non-East Asians with postmenopausal osteoporosis. MEDLINE, EMBASE, and Cochrane CENTRAL were searched for relevant randomized controlled trials (RCTs) comparing the efficacy of alendronate versus placebo (or calcium/mineral and/or Vitamin D or hormone replacement therapy) in primary postmenopausal osteoporotic women. We calculated the weighted mean differences (WMDs) for lumbar spinal BMD and the risk ratios (RRs) for vertebral fracture risk along with their respective 95% confidence intervals (CIs). From an initial set of 445 non-duplicate records, 13 full-text articles were finally included in this meta-analysis consisting of four East Asian RCTs and nine non-East Asian RCTs. Alendronate therapy displayed significant effects in improving lumbar spinal BMD in both East Asians [WMD (95% CI)=5.30 (0.32–10.29), p=0.037] and non-East Asians [WMD (95% CI)=5.73 (3.61–7.85), p=0.000]. Alendronate therapy did not display significant effects upon vertebral fracture risk in East Asians [RR (95% CI)=0.41 (0.06–2.73), p=0.358] but did display a significant effect upon lowering vertebral fracture risk in non-East Asians [RR (95% CI)=0.55 (0.42–0.72), p=0.000]. These findings suggest that ethnicity may affect the efficacy of bisphosphonate therapy in postmenopausal osteoporotic women.
The Clinical Significance of Fibrin Monomers
Authors: Refaai Majed , Riley Paul , Mardovina Tatsiana , Bell Phoenix 
Published: October 2018

Introduction Fibrin monomer (FM) concentrations reflect pro-thrombin activity and have the potential to predict thrombotic events relatively earlier than other haemostatic markers. Most often, FM are compared with D-dimer (DD) as increased DD have been documented in disseminated intravascular coagulation (DIC), deep vein thrombosis (DVT) and pulmonary embolism. Although DD have a high sensitivity and negative predictive value, their specificity is much lower depending on the assay chosen, clinical pre-test probability and patient condition. There are limited reports investigating the utility of FM in hyper-coagulable patients.
Methods We performed a literature search of FM concentrations in hyper-coagulable patients including those with DIC, acute ischaemic stroke, atrial fibrillation, acute myocardial infarction, venous thromboembolism (VTE) and cancer, as well as those who are pregnant or undergoing surgery.
Results FM were increased in patients with DIC and those with malignancy. In contrast, detection of VTE or post-operative DVT development is likely enhanced using both FM and DD concentrations. Similarly, measuring FM concentrations with other biomarker levels may be more beneficial in patients suffering an acute myocardial infarction or acute ischaemic stroke. Lastly, FM concentrations vary substantially throughout pregnancy with no definitive role of FM as of yet.
Conclusion Utilizing FM concentrations to assess hyper-coagulable patients seems promising; however, there are limitations including variations in FM cut-off values, the effect of patient medications and the timing of FM measurement relative to an acute event. Thus, further investigation is required before a true advantage for FM as a haemostatic marker can be established.
From second language to third language learning
Authors: Man Laura , Bui Gavin , Teng Mark Feng 
Published: October 2018

AbstractThis mixed-methods study explores English and Japanese learning motivations in a group of Japanese summer course participants at a university in Hong Kong. Sixty-one Cantonese-speaking students completed two questionnaire surveys on co-existing motivations for learning L2 English and L3 Japanese. Depending on the questionnaire results, eight participants were selected to take part in the two subsequent interviews. The findings revealed that the participants perceived L2 English learning as a school subject and a practical tool for academics and career. It was instrumentality, rather than integrativeness, that motivated the students to learn English. Contrary results were found for L3 Japanese language learning, as the learners had more cultural interest, positive learning experience, self-confidence, and positive attitudes. This study, therefore, argues that L2 and L3 motivations merit distinct status. There appears to exist a dual-motivation system amongst these multilingual learners. The study also provides insights into how geographical and psychological distances could impact language learning under the phenomena of globalisation.
The stereotypicality of symptomatic and pragmatic argumentation in consultations about palliative systemic treatment for advanced cancer
Authors: Akkermans Aranka , Snoeck Henkemans Francisca , Labrie Nanon , Henselmans Inge , van Laarhoven Hanneke 
Published: October 2018

Abstract
A recent line of argumentation research has focused on the examination of prototypical argumentative patterns – patterns that can
be theoretically expected in view of the type of standpoint defended, the institutional aim, and the conventions and constraints
of the context (Van Eemeren 2016: 13–15). This paper aims to add a new dimension to
both this line of research and research on health communication by determining whether the prototypical types of argumentation in
consultations about palliative systemic treatment for advanced cancer are stereotypical as well, that is, whether they are
dominant in a quantitative sense (van Eemeren 2016: 16). For this purpose, a valid and
reliable measurement instrument is developed and used in a content analysis of the transcripts of 49 consultations. On the basis
of the results of this analysis, it can be concluded that the use of symptomatic and pragmatic argumentation is stereotypical in
this type of consultations.
Donald Trump’s rhetoric
Authors: Kayam Orly 
Published: October 2018

AbstractThe paper explores the rhetorical strategies Donald Trump employed during the 2016 U.S. presidential primary campaign. The study shows that Trump aimed at garnering public support by defining himself as an anti-politician or anti-political establishment candidate. His rhetorical strategies were aimed at building the depiction of his character as a successful businessman who came from outside the political realm to save America and restore it to its former greatness. He denounced the traditional rules of politics, avoided calculated, logical and politically correct utterances, and modeled himself as the only candidate who was fit for the presidency. The analysis reveals Trump’s prominent rhetorical strategies, and shows how each one of them fulfilled what I refer to as the ultimate “Anti-Political Rhetorical Strategy”, from ananti-politically correctstrategy, which is by nature anti-political, to more common strategies such asnegativity,simplicity, repetitionandhyperbole.