bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns of Non-Invasive Ventilation in Amyotrophic Lateral Sclerosis
Authors: Markovic Nevena , Povitz Marcus , Smith Joanne , Leasa David , Shoesmith Christen , Gofton Teneille E. 
Published: June 2018

AbstractBackground: Non-invasive ventilation (NIV) improves quality of life and survival in patients with amyotrophic lateral sclerosis (ALS) and respiratory symptoms. Little is known about the patterns of NIV use over time and the impact of NIV on end-of-life decision-making in ALS. Objective: This study assessed the pattern of NIV use over the course of the disease and the timing of end-of-life discussions in people living with ALS. Method: A retrospective single-center cohort study was performed at London Health Sciences Centre. Daily NIV duration of use was evaluated at 3-month intervals. The timing of diagnosis, NIV initiation, discussions relating to do-not-attempt-resuscitation (DNAR) and death were examined. Results: In total, 48 patients were included in the analysis. Duration of NIV use increased over time, and tolerance to NIV was observed to be better than expected in patients with bulbar-onset ALS. There was a high degree of variability in the timing of end-of-life discussions in patients with ALS (356±451 days from diagnosis). In this cohort, there was a strong association between the timing of discussions regarding code status and establishment of a DNAR order (r2=0.93). Conclusion: This retrospective cohort study suggests that the use of NIV in ALS increases over time and that there remains a great deal of variability in the timing of end-of-life discussions in people living with ALS. Future prospective studies exploring the use NIV over the disease trajectory and how NIV affects end-of-life decision-making in people with ALS are needed.
Screening for soybean varieties suited to Belgian growing conditions based on maturity, yield components and resistance to Sclerotinia sclerotiorum and Rhizoctonia solani anastomosis group 2-2IIIB
Authors: Pannecoucque J. , Goormachtigh S. , Heungens K. , Vleugels T. , Ceusters J. , Van Waes C. , Van Waes J. 
Published: June 2018

AbstractPolicy makers and farmers in north-west Europe are expressing a growing interest in soybean production. However, cool and wet climatic conditions in this region pose challenges for this crop in terms of reaching maturity and producing sufficient yield and create additional disease pressure from the fungal pathogens Sclerotinia sclerotiorum and Rhizoctonia solani. To increase the chance for successful introduction of this new crop in Belgium and to determine the main issues for local soybean breeding programmes, 14 early maturing varieties were screened over a 2-year-period for their agronomic performance. Based on novel bioassays, susceptibility to S. sclerotiorum and R. solani anastomosis group (AG) 2-2IIIB was evaluated. The varieties tested were able to reach sufficient maturity (average seed moisture content of 19.0%) by the beginning of October. Significant differences were observed in most agronomic characteristics, with seed yield and protein content ranging from 2002 to 2916 kg dry matter/ha and 35.5–43.3%, respectively. Taller varieties ripened later but reached higher protein levels compared with shorter varieties. Tolerance to lodging was correlated with seed and protein yield but was not correlated with plant height. Large seeds corresponded with a high protein content. Susceptibility to S. sclerotiorum reflected significant differences between varieties. In contrast, levels of susceptibility to R. solani AG 2-2IIIB were similar between most varieties, with only the variety Primus showing significantly less disease. The results of the current study hold promise for a successful introduction of soybean cultivation in north-west Europe and areas for further crop improvement have been identified.
Depleting fragile bodies: the political economy of sexual and reproductive health in crisis situations
Authors: Tanyag Maria 
Published: June 2018

AbstractIn a crisis-prone world, the number of internally displaced persons (IDPs) uprooted by both armed conflicts and environmental disasters has drastically increased and displacement risks have intensified. Despite the growing attention within global security and development agendas to sexual and reproductive health and rights (SRHR), there remain striking gaps in addressing SRHR in crisis situations, particularly among IDP women and girls. This article examines the continuum between social reproduction in times of crisis and the material and ideological conditions that restrict women’s bodily autonomy in everyday life. Using the case of the Philippines where millions of people are routinely affected by conflict and disaster-induced displacements, it argues that the failure to recognise the centrality of women’s health and bodily autonomy not only hinders the sustainable provision of care and domestic labour during and after crisis, but also fundamentally constrains how security is enacted within these spaces. Thus, the article highlights an urgent need to rethink the gendered political economy of crisis responses as a building block for stemming gendered violence and depletion of social reproductive labour at the household, state, and global levels.
Emerging word segmentation abilities in European Portuguese-learning infants: new evidence for the rhythmic unit and the edge factor
Authors: BUTLER Joseph , FROTA Sónia 
Published: June 2018

AbstractWord segmentation plays a crucial role in language acquisition, particularly for word learning and syntax development, and possibly predicts later language abilities. Previous studies have suggested that this ability develops differently across languages, possibly affected by the languages’ rhythmic properties (Rhythmic Segmentation Hypothesis) and target word location in the prosodic structure (Edge Hypothesis). The present study investigates early word segmentation in a language, European Portuguese, that exhibits both stress- and syllable-timed properties, as well as strong cues to both higher-level prosodic boundaries and the word level. Infants aged 4–10 months old were tested with target words located in utterance-medial and utterance-final positions. Evidence for word segmentation was found early in development but only for utterance-edge located target words, suggesting the more salient prosodic cues play a crucial role. There was some evidence for segmentation in utterance-medial position by 10 months, demonstrating that this ability is not yet fully developed, possibly due to mixed rhythmic properties.
Working with interpretations in cognitive behavioural therapy for obsessive compulsive disorder
Authors: Jones Gazal , Wroe Abigail , Jezard Lucy , Jefferys Georgina , Brown Gary 
Published: June 2018

AbstractCognitive behavioural therapy (CBT) is a highly effective treatment for obsessive compulsive disorder (OCD). Identifying, challenging and monitoring interpretations of intrusions is considered a key element of CBT for OCD but preliminary research suggests that treatment does not always include identification and modification of misinterpretations. The present investigation explored ‘OCD-expert’ and ‘non-OCD-expert’ clinicians’ views on key elements of CBT for OCD to determine whether identifying and modifying key interpretations were considered important in therapy and whether clinicians who do not have specific expertise in OCD found working with interpretations difficult. Study 1 used a qualitative approach to investigate OCD-expert and non-OCD-expert clinician's views on key elements of CBT for OCD. Study 2 used a questionnaire to investigate what non-OCD-expert clinicians viewed as important and difficult aspects of CBT for OCD. Study 1 results showed that OCD-expert and non-OCD-expert clinicians reported working with interpretations was a key element of CBT for OCD. However, OCD-expert clinicians linked interpretations more closely to a formulation and intervention plan and reported using more techniques and questionnaires when working with interpretations compared with non-OCD-expert clinicians. Study 2 results showed that non-OCD-expert clinicians rated interpretations as both important and difficult to work with but no more important or difficult than other key elements of CBT for OCD. OCD-expert and non-OCD-expert clinicians identify working with interpretations as a key element of CBT for OCD. Non-OCD-expert clinicians may benefit from additional training on formulation tools that help identify, monitor and challenge interpretations of intrusions.
Identification of Hydrobiogeochemical Processes Controlling Seasonal Variations in Arsenic Concentrations Within a Riverbank Aquifer at Jianghan Plain, China
Authors: Huang Kun , Liu Yuanyuan , Yang Chen , Duan Yanhua , Yang Xiaofan , Liu Chongxuan 
Published: June 2018

AbstractThe arsenic concentration in groundwater varies significantly with time and space in groundwater‐surface water exchange zones. Various processes have been identified that control arsenic concentration distribution and mobility in laboratory systems. However, it is still challenging to identify important processes controlling arsenic concentration distribution at the field scale due to the complex coupling of hydrobiogeochemical processes. In this study, a reactive flow and transport model was used to identify dominant processes controlling arsenic distribution and seasonal variations in groundwater‐surface water exchange zones using Jianghan Plain, China, as an example. The results revealed the importance of river water and groundwater interactions on seasonal changes in arsenic concentration; however, the affected region is limited to within 50‐m distance to the river. The modeling results, unexpectedly, revealed the predominant importance of groundwater extraction to the seasonal variation in arsenic concentration. The groundwater extraction changed the groundwater flow pattern and induced vertical leakage of oxygen‐containing surface water into the aquifer, which triggered a series of biogeochemical reactions that changed groundwater redox conditions and promoted arsenic sorption, resulting in a rapid decrease in arsenic concentrations in groundwater. After groundwater extraction ceased, aquifer recovered to anoxic condition, promoting arsenic release from the sorbed phase, leading to a rapid rebounding in groundwater arsenic concentrations. Overall, this study provided a tool to identify coupled hydrobiogeochemical processes on arsenic spatiotemporal distribution and migration in groundwater.
The Role of Capillary Hysteresis and Pore‐Scale Heterogeneity in Limiting the Migration of Buoyant Immiscible Fluids in Porous Media
Authors: Cihan Abdullah , Wang Shibo , Tokunaga Tetsu K. , Birkholzer Jens T. 
Published: June 2018

AbstractUnderstanding the main mechanisms affecting long‐term migration and redistribution of injected CO2 in geological carbon storage is needed for developing predictive models to assess environmental risks and designing monitoring schemes. Preparation of a postinjection site care plan is required for CO2 injection wells, including monitoring of pressure changes and injected CO2 plume. Knowledge gaps exist regarding assessment of postinjection monitoring timeframes for the CO2 plume because the processes driving long‐term CO2 plume migration and trapping are not fully understood. In the postinjection stage of geological carbon storage, redistribution of CO2 occurs mainly due to buoyancy and capillary forces. This work presents experimental and modeling studies to investigate processes contributing to postinjection plume distribution and stabilization. We conducted a flow cell experiments (0.5 m × 0.05 m × 0.01 m) with two immiscible fluid phases in a glass bead porous medium to study postinjection plume behavior. We employed a hysteretic macroscopic two‐phase flow model to interpret the experimental results and to understand main processes leading to plume stabilization. Our findings show that capillary pressure hysteresis explains the experimentally observed plume shape and redistribution at early postinjection stages; however, the long‐term plume migration and eventual plume stabilization can only be represented when in addition microscale heterogeneity is accounted for. Results also show that plume stabilization can be extremely slow and that the migration of the plume front can occur through multiple intermittent bursts over long times. Further studies are needed to understand implications of the results for more realistic porous media and large‐scale storage reservoirs.
Caspr1 is a host receptor for meningitis-causing Escherichia coli
Authors: Zhao Wei-Dong , Liu Dong-Xin , Wei Jia-Yi , Miao Zi-Wei , Zhang Ke , Su Zheng-Kang , Zhang Xue-Wei , Li Qiang , Fang Wen-Gang , Qin Xiao-Xue , Shang De-Shu , Li Bo , Li Qing-Chang , Cao Liu , Kim Kwang Sik , Chen Yu-Hua 
Published: June 2018

AbstractEscherichia coli is the leading cause of neonatal Gram-negative bacterial meningitis, but the pathogenesis of E. coli meningitis remains elusive. E. coli penetration of the blood–brain barrier (BBB) is the critical step for development of meningitis. Here, we identify Caspr1, a single-pass transmembrane protein, as a host receptor for E. coli virulence factor IbeA to facilitate BBB penetration. Genetic ablation of endothelial Caspr1 and blocking IbeA–Caspr1 interaction effectively prevent E. coli penetration into the brain during meningitis in rodents. IbeA interacts with extracellular domain of Caspr1 to activate focal adhesion kinase signaling causing E. coli internalization into the brain endothelial cells of BBB. E. coli can invade hippocampal neurons causing apoptosis dependent on IbeA–Caspr1 interaction. Our results indicate that E. coli exploits Caspr1 as a host receptor for penetration of BBB resulting in meningitis, and that Caspr1 might be a useful target for prevention or therapy of E. coli meningitis.
Dishevelled has a YAP nuclear export function in a tumor suppressor context-dependent manner
Authors: Lee Yoonmi , Kim Nam Hee , Cho Eunae Sandra , Yang Ji Hye , Cha Yong Hoon , Kang Hee Eun , Yun Jun Seop , Cho Sue Bean , Lee Seon-Hyeong , Paclikova Petra , Radaszkiewicz Tomasz W. , Bryja Vitezslav , Kang Chi Gu , Yuk Young Soo , Cha So Young , Kim Soo-Youl , Kim Hyun Sil , Yook Jong In 
Published: June 2018

AbstractPhosphorylation-dependent YAP translocation is a well-known intracellular mechanism of the Hippo pathway; however, the molecular effectors governing YAP cytoplasmic translocation remains undefined. Recent findings indicate that oncogenic YAP paradoxically suppresses Wnt activity. Here, we show that Wnt scaffolding protein Dishevelled (DVL) is responsible for cytosolic translocation of phosphorylated YAP. Mutational inactivation of the nuclear export signal embedded in DVL leads to nuclear YAP retention, with an increase in TEAD transcriptional activity. DVL is also required for YAP subcellular localization induced by E-cadherin, α-catenin, or AMPK activation. Importantly, the nuclear-cytoplasmic trafficking is dependent on the p53-Lats2 or LKB1-AMPK tumor suppressor axes, which determine YAP phosphorylation status. In vivo and clinical data support that the loss of p53 or LKB1 relieves DVL-linked reciprocal inhibition between the Wnt and nuclear YAP activity. Our observations provide mechanistic insights into controlled proliferation coupled with epithelial polarity during development and human cancer.
Automatic detection advantage of network information among Internet addicts: behavioral and ERP evidence
Authors: He Jinbo , Zheng Yang , Nie Yufeng , Zhou Zongkui 
Published: June 2018

AbstractConverging evidence has proved the attentional bias of Internet addicts (IAs) on network information. However, previous studies have neither explained how characteristics of network information are detected by IAs with priority nor proved whether this advantage is in line with the unconscious and automatic process. To answer the two questions, this study aims to investigate whether IAs prioritize automatic detection of network information from the behavior and cognitive neuroscience aspects. 15 severe IAs and 15 matching healthy controls were selected using Internet Addiction Test (IAT). Dot-probe task with mask was used in the behavioral experiment, while deviant-standard reverse oddball paradigm was used in the event-related potential (ERP) experiment to induce mismatch negativity (MMN). In the dot-probe task, when the probe location appeared on the Internet-related picture’s position, the IAs had significantly shorter reaction time than do the controls; in the ERP experiment, when Internet-related picture appeared, MMN was significantly induced in the IAs relative to the controls. Both experiments show that IAs can automatically detect network information.
Molecular profiles and tumor mutational burden analysis in Chinese patients with gynecologic cancers
Authors: Wang Min , Fan Wensheng , Ye Mingxia , Tian Chen , Zhao Lili , Wang Jianfei , Han Wenbo , Yang Wen , Gu Chenglei , Li Mingxia , Zhang Zhe , Wang Yongjun , Zhang Henghui , Meng Yuanguang 
Published: June 2018

AbstractThe goal of this work was to investigate the tumor mutational burden (TMB) in Chinese patients with gynecologic cancer. In total, 117 patients with gynecologic cancers were included in this study. Both tumor DNA and paired blood cell genomic DNA were isolated from formalin-fixed paraffin-embedded (FFPE) specimens and blood samples, and next-generation sequencing was performed to identify somatic mutations. TP53, PTEN, ARID1A, and PIK3CA alterations were significantly different in various types of gynecologic cancers (p = 0.001, 1.15E-07, 0.004, and 0.009, respectively). The median TMB of all 117 gynecologic tumor specimens was 0.37 mutations/Mb, with a range of 0–41.45 mutations/Mb. Despite the lack of significant difference, endometrial cancer cases had a higher median TMB than cervical and ovarian cancer cases. Younger gynecologic cancer patients (age <40 years) had a significantly lower TMB than older patients (age ≥40 years) (p = 0.04). In addition, TMB was significantly increased with increasing clinical stage of disease (p = 0.001). PTEN alterations were commonly observed in patients with a moderate to high TMB (n = 8, 38.10%, p = 9.95E-04). Although limited by sample size, all of the patients with TSC2 (n = 3, p = 3.83E-11) or POLE (n = 2, p = 0.005) mutations had a moderate to high TMB. Further large-scale, prospective studies are needed to validate our findings.
An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease
Authors: Zhao Yue , Wang Chunhong , Wang Cong , Hong Xue , Miao Jinhua , Liao Yulin , Zhou Lili , Liu Youhua 
Published: June 2018

AbstractActivation of the renin-angiotensin system (RAS) is associated with hypertension and heart disease. However, how RAS activation causes cardiac lesions remains elusive. Here we report the involvement of Wnt/β-catenin signaling in this process. In rats with chronic infusion of angiotensin II (Ang II), eight Wnt ligands were induced and β-catenin activated in both cardiomyocytes and cardiac fibroblasts. Blockade of Wnt/β-catenin signaling by small molecule inhibitor ICG-001 restrained Ang II-induced cardiac hypertrophy by normalizing heart size and inhibiting hypertrophic marker genes. ICG-001 also attenuated myocardial fibrosis and inhibited α-smooth muscle actin, fibronectin and collagen I expression. These changes were accompanied by a reduced expression of atrial natriuretic peptide and B-type natriuretic peptide. Interestingly, ICG-001 also lowered blood pressure induced by Ang II. In vitro, Ang II induced multiple Wnt ligands and activated β-catenin in rat primary cardiomyocytes and fibroblasts. ICG-001 inhibited myocyte hypertrophy and Snail1, c-Myc and atrial natriuretic peptide expression, and abolished the fibrogenic effect of Ang II in cardiac fibroblasts. Finally, recombinant Wnt3a was sufficient to induce cardiomyocyte injury and fibroblast activation in vitro. Taken together, these results illustrate an essential role for Wnt/β-catenin in mediating hypertension, cardiac hypertrophy and myocardial fibrosis. Therefore, blockade of this pathway may be a novel strategy for ameliorating hypertensive heart disease.
Iron-Terephthalate Coordination Network Thin Films Through In-Situ Atomic/Molecular Layer Deposition
Authors: Tanskanen A. , Karppinen M. 
Published: June 2018

AbstractIron terephthalate coordination network thin films can be fabricated using the state-of-the-art gas-phase atomic/molecular layer deposition (ALD/MLD) technique in a highly controlled manner. Iron is an Earth-abundant and nonhazardous transition metal, and with its rich variety of potential applications an interesting metal constituent for the inorganic-organic coordination network films. Our work underlines the role of the metal precursor used when aiming at in-situ ALD/MLD growth of crystalline inorganic-organic thin films. We obtain crystalline iron terephthalate films when FeCl3 is employed as the iron source whereas depositions based on the bulkier Fe(acac)3 precursor yield amorphous films. The chemical composition and structure of the films are investigated with GIXRD, XRR, FTIR and XPS.
Heterologous expression of CTP:phosphocholine cytidylyltransferase from Plasmodium falciparum rescues Chinese Hamster Ovary cells deficient in the Kennedy phosphatidylcholine biosynthesis pathway
Authors: Marton Lívia , Hajdú Fanni , Nagy Gergely N. , Kucsma Nóra , Szakács Gergely , Vértessy Beáta G. 
Published: June 2018

AbstractThe plasmodial CTP:phosphocholine cytidylyltransferase (PfCCT) is a promising antimalarial target, which can be inhibited to exploit the need for increased lipid biosynthesis during the erythrocytic life stage of Plasmodium falciparum. Notable structural and regulatory differences of plasmodial and mammalian CCTs offer the possibility to develop species-specific inhibitors. The aim of this study was to use CHO-MT58 cells expressing a temperature-sensitive mutant CCT for the functional characterization of PfCCT. We show that heterologous expression of wild type PfCCT restores the viability of CHO-MT58 cells at non-permissive (40 °C) temperatures, whereas catalytically perturbed or structurally destabilized PfCCT variants fail to provide rescue. Detailed in vitro characterization indicates that the H630N mutation diminishes the catalytic rate constant of PfCCT. The flow cytometry-based rescue assay provides a quantitative readout of the PfCCT function opening the possibility for the functional analysis of PfCCT and the high throughput screening of antimalarial compounds targeting plasmodial CCT.
Role of recombination and faithfulness to partner in sex chromosome degeneration
Authors: Mackiewicz Dorota , Posacki Piotr , Burdukiewicz Michał , Błażej Paweł 
Published: June 2018

AbstractSex determination in mammals is strongly linked to sex chromosomes. In most cases, females possess two copies of X chromosome while males have one X and one Y chromosome. It is assumed that these chromosomes originated from a pair of homologous autosomes, which diverged when recombination between them was suppressed. However, it is still debated why the sex chromosomes stopped recombining and how this process spread out over most part of the chromosomes. To study this problem, we developed a simulation model, in which the recombination rate between the sex chromosomes can freely evolve. We found that the suppression of recombination between the X and Y is spontaneous and proceeds very quickly during the evolution of population, which leads to the degeneration of the Y in males. Interestingly, the degeneration happens only when mating pairs are unfaithful. This evolutionary strategy purifies the X chromosome from defective alleles and leads to the larger number of females than males in the population. In consequence, the reproductive potential of the whole population increases. Our results imply that both the suppression of recombination and the degeneration of Y chromosome may be associated with reproductive strategy and favoured in polygamous populations with faithless mating partners.