bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptional and post-transcriptional regulation and transcriptional memory of chromatin regulators in response to low temperature
Authors: Vyse K. , Faivre L. , Romich M. , Pagter M. , Schubert D. , Hincha D.K. , Zuther E. 
Published: September 2019

AbstractChromatin regulation ensures stable repression of stress-inducible genes under non-stress conditions and transcriptional activation and memory of such an activation of those genes when plants are exposed to stress. However, there is only limited knowledge on how chromatin genes are regulated at the transcriptional and post-transcriptional level upon stress exposure and relief from stress. We have therefore set-up a RT-qPCR-based platform for high-throughput transcriptional profiling of a large set of chromatin genes. We find that the expression of a large fraction of these genes is regulated by cold. In addition, we reveal an induction of several DNA and histone demethylase genes and certain histone variants after plants have been shifted back to ambient temperature (deacclimation), suggesting a role in the memory of cold acclimation. We also re-analyse large scale transcriptomic datasets for transcriptional regulation and alternative splicing (AS) of chromatin genes, uncovering an unexpected level of regulation of these genes, particularly at the splicing level. This includes several vernalization regulating genes whose AS results in cold-regulated protein diversity. Overall, we provide a profiling platform for the analysis of chromatin regulatory genes and integrative analyses of their regulation, suggesting a dynamic regulation of key chromatin genes in response to low temperature stress.
Proteomic analysis of the desmosome identifies novel components required for skin integrity
Authors: Badu-Nkansah Kwabena , Lechler Terry 
Published: September 2019

SummaryDesmosomes are cell-cell adhesions necessary for the maintenance of tissue integrity in the skin and the heart. While the core components of the desmosome have been identified, peripheral components that modulate canonical desmosome functions or that have noncanonical roles remain largely unexplored. Here we used targeted proximity labeling approaches to elaborate the desmosome proteome in epidermal keratinocytes. Quantitative mass spectrometry analysis identified all core desmosome proteins while uncovering a diverse network of new constituents with broad molecular functions. By individually targeting the inner and outer dense plaques, we additionally define proteins enriched in these subcompartments. We validated a number of these novel desmosome-associated proteins and find that many show a dependence upon the core desmosomal protein, desmoplakin, for their localization. We further explored the mechanism of localization and function of two novel desmosome-associated adaptor proteins that we identified, Crk and Crkl. These proteins interacted with Dsg1 and require both Dsg1 and desmoplakin for robust cortical localization. Epidermal deletion of both Crk and CrkL in mice resulted in perinatal lethality with defects in desmosome morphology and keratin organization, thus demonstrating the utility of this dataset in identifying novel proteins required for desmosome-dependent epidermal integrity.
Miswired enhancer logic drives translocation positive rhabdomyosarcoma
Authors: Gryder Berkley E. , Wachtel Marco , Chang Kenneth , El Demerdash Osama , Aboreden Nicholas G. , Mohammed Wardah , Ewert Winston , Pomella Silvia , Rota Rossella , Wei Jun S. , Song Young , Schaefer Beat , Vakoc Christopher R. , Khan Javed 
Published: September 2019

AbstractCore regularity transcription factors (CR TFs) define cell identity and lineage through an exquisitely precise and logical order during embryogenesis and development. These CR TFs regulate one another in three-dimensional space via distal enhancers that serve as logic gates embedded in their TF recognition sequences. Aberrant chromatin organization resulting in miswired circuitry of enhancer logic is a newly recognized feature in many cancers. Here, we report that PAX3-FOXO1 expression is driven by a translocated FOXO1 distal super enhancer (SE). Using 4C-seq, a technique detecting all genomic regions that interact with the translocated FOXO1 SE, we demonstrate its physical interaction with the PAX3 promotor only in the presence of the oncogenic translocation. Furthermore, RNA-seq and ChIP-seq in tumors bearing rare PAX translocations implicate enhancer miswiring is a pervasive feature across all FP-RMS tumors. HiChIP of enhancer mark H3K27ac showed extended connectivity between the distal FOXO1 SE and additional intra-domain enhancers and the PAX3 promoter. We show by CRISPR-paired-ChIP-Rx that PAX3-FOXO1 transcription is diminished when this network of enhancers is selectively ablated. Therefore, our data reveal a mechanism of a translocated hijacked enhancer which disrupts the normal CR TF logic during skeletal muscle development (PAX3 to MYOD to MYOG), replacing it with an infinite loop logic that locks rhabdomyosarcoma cells in an undifferentiated proliferating stage.
Suppression of p53 response by targeting p53-Mediator binding with a stapled peptide
Authors: Allen BL , Quach K , Levandowski CB , Rubin JD , Read T , Dowell RD , Schepartz A , Taatjes DJ 
Published: September 2019

AbstractDNA-binding transcription factors (TFs) remain challenging to target with molecular probes. Many TFs function in part through interaction with Mediator; we sought to block p53 function by disrupting the p53-Mediator interaction. Through rational design and activity-based screening, we characterized a stapled peptide, with functional mimics of both p53 activation domains, that selectively inhibited p53- and Mediator-dependent transcription in vitro. This “bivalent peptide” also suppressed p53 transcriptional response in human cancer cells. Our strategy circumvents the TF and instead targets the TF-Mediator interface, with desired transcriptional outcomes. Different TFs target Mediator through different subunits, suggesting this strategy could be broadly applied.
Control of clathrin-mediated endocytosis by NIMA family kinases
Authors: Joseph Braveen B. , Wang Yu , Edeen Phil , Lažetić Vladimir , Grant Barth D. , Fay David S. 
Published: September 2019

AbstractEndocytosis, the process by which cells internalize plasma membrane and associated cargo, is regulated extensively by posttranslational modifications. Previous studies suggested the potential involvement of scores of protein kinases in endocytic control, of which only a few have been validated within their native context. Here we show that the conserved NIMA-related kinases NEKL-2/NEK8/9 and NEKL-3/NEK6/7 (the NEKLs) control clathrin-mediated endocytosis inC. elegans. Loss of NEKLs leads to clathrin mislocalization and to a dramatic reduction in clathrin mobility at the apical membrane. Strikingly, reducing the levels of active AP2, the major clathrin adapter complex, rescuesneklmutant defects, whereas increased levels of active AP2 exacerbate nekl defects. Moreover, NEKL inhibition alleviates defects associated with reduced AP2 activity, attesting to the tight link between NEKL and AP2 functions. We also show that NEKLs are required for the clustering and internalization of membrane cargo and that human NEKs rescue defects inneklmutant worms.
Tolerance–reliability analysis of mechanical assemblies for quality control based on Bayesian modeling
Authors: Khodaygan S. , Ghaderi A. 
Published: September 2019

PurposeThe purpose of this paper is to present a new efficient method for the tolerance–reliability analysis and quality control of complex nonlinear assemblies where explicit assembly functions are difficult or impossible to extract based on Bayesian modeling.Design/methodology/approachIn the proposed method, first, tolerances are modelled as the random uncertain variables. Then, based on the assembly data, the explicit assembly function can be expressed by the Bayesian model in terms of manufacturing and assembly tolerances. According to the obtained assembly tolerance, reliability of the mechanical assembly to meet the assembly requirement can be estimated by a proper first-order reliability method.FindingsThe Bayesian modeling leads to an appropriate assembly function for the tolerance and reliability analysis of mechanical assemblies for assessment of the assembly quality, by evaluation of the assembly requirement(s) at the key characteristics in the assembly process. The efficiency of the proposed method by considering a case study has been illustrated and validated by comparison to Monte Carlo simulations.Practical implicationsThe method is practically easy to be automated for use within CAD/CAM software for the assembly quality control in industrial applications.Originality/valueBayesian modeling for tolerance–reliability analysis of mechanical assemblies, which has not been previously considered in the literature, is a potentially interesting concept that can be extended to other corresponding fields of the tolerance design and the quality control.
The prevention of offending behaviour by people with intellectual disabilities: a case for specialist childhood and adolescent early intervention
Authors: Chester Verity , Wells Harriet , Lovell Mark , Melvin Clare , Tromans Samuel Joseph 
Published: September 2019

Purpose
Elucidating where antisocial or violent behaviour arises within the life course of individuals with intellectual disability (ID) could improve outcomes within this population, through informing services and interventions which prevent behaviours reaching a forensic threshold. The paper aims to discuss this issue.

Design/methodology/approach
The Historical Clinical Risk Management-20, Version 3 assessments of a cohort of 84 inpatients within a forensic ID service were analysed for this study, with a particular emphasis on items concerned with the age at which antisocial or violence first emerged.

Findings
For most participants, violent or antisocial behaviour was first observed in childhood or adolescence. The study also highlighted a smaller subgroup, whose problems with violence or antisocial behaviour were first observed in adulthood.

Originality/value
The study findings suggest that targeted services in childhood and adolescence may have a role in reducing the offending behaviour and forensic involvement of people with ID. This has implications for the service models provided for children and adolescents with ID with challenging or offending behaviour.
Hedonism or self-growth? The influence of perceived control on individual product preferences for individuals under self-threat
Authors: Zhao Taiyang , Song Wei , Jin Xiaotong , Cui Hongjing , Li Yang 
Published: September 2019

PurposeThe purpose of this paper is to study the influence of perceived control on product preferences of consumers under self-threat.Design/methodology/approachBy conducting four experiments, this paper manipulated the participants’ self-threat and three sources of perceived control – namely, the controllability of the threat itself, the internal and external locus of control of the individual and priming the existing positive or negative experience of the individuals. After the manipulations, the participants’ product preferences for self-growth goods or hedonic goods were measured.FindingsThe findings of this research indicated that when consumers have a higher perceived control of the threats they are facing, they are more likely to adopt problem-focused coping strategies and show a preference for self-growth goods, which can help them resolve their threats. However, when consumers have a lower perceived control on the threats they are facing, they are more likely to adopt emotion-focused coping strategies and show a preference for hedonic goods, which can help them adjust their emotions.Originality/valueThis paper systematically confirms the interactive effect of perceived control and self-threat on consumers’ product preferences, as well as the meditation role of problem-focused coping and emotion-focused coping. It also provides insights for marketers to know what factors may affect consumers’ preferences for products in self-threat situations, thus contributing to marketing practices.
Regulating mediators of internet piracy: P2P websites and cyberlockers
Authors: Walter Jason M. 
Published: September 2019

PurposeContemporary copyright infringement has significantly changed in the digital era, and because of the unique attributes of internet piracy and method of exchange, traditional regulatory approaches are ineffective. The characteristics of digital goods enable users to almost costlessly copy and exchange content. Much of the contemporary research fails to incorporate the necessary components of exchange that are central to digital piracy. This paper aims to examine the role of peer-to-peer network hosts and the often-omitted cyberlocker.Design/methodology/approachA simple framework is constructed that describes how these entities how these entities facilitate digital piracy and operate financially. This framework illustrates the objectives of piracy mediators, highlighting the avenues by which regulation can craft policy. Additional examination of online piracy highlights the challenges of contemporary policy to combat digital piracy due to the secondary consequences.FindingsRecent policies, aimed at diminishing piracy, would infringe on consumers’ privacy, hurt business finances or strategically used by rivals to hurt the operations of legal entities. Trying to prevent illegal sources (or facilitators) of pirate goods from providing access to files continues to be challenging. In many instances, the blurry line between a legal platform for file exchange and a piracy haven creates significant regulatory problems. For known piracy promoters (host sites or cyberlockers), location and revenue streams continue to limit direct intervention.Originality/valueThis paper discusses the necessary path for piracy to occur by including previously omitted agents necessary for communication and/or distribution. My analysis incorporates these entities that facilitate piracy and the unique features of digital exchange, which has industrial and regulatory implications. Furthermore, my results highlight why regulators have been ineffective in crafting meaningful anti-piracy policy.
Cultural societies and information needs: Croats in New Zealand
Authors: Krtalić Maja , Hebrang Grgić Ivana 
Published: September 2019

Purpose
The purpose of this paper was to explore how small immigrant communities in host countries collect, disseminate and present information about their home country and their community, and the role of formal societies and clubs in it.

Design/methodology/approach
This paper presents the results of a case study of the Croatian community in New Zealand. To illustrate how cultural and technological changes affected information dissemination and communication within the community, the case study presents both historical and current situations. Methods used in this case study included a content analysis of historical newspapers published in New Zealand by the Croatian community, content analysis of current webpages and social networking sites, and interviews with participants who have management roles in Croatian societies and communities in New Zealand. Data were collected from December 2018 to February 2019.

Findings
Formally established clubs and societies, but also informal groups of immigrants and their descendants can play a significant role in providing their members with information about the culture, social life and events of the home country. They also play a significant role in preserving part of the history and heritage which is relevant, not only for a specific community but also for the history and culture of a home country.

Originality/value
The methodology used in the research is based on data from community archives and can be used for studying other small immigrant communities in New Zealand or abroad. The case study presented in the paper illustrates how the information environment of small immigrant communities develops and changes over the years under the influence of diverse political, social and technological changes.
Understanding the building of professional identities with the LEGO® SERIOUS PLAY® method using situational mapping and analysis
Authors: Hayes Catherine , Graham Yitka 
Published: September 2019

PurposeThe purpose of this paper is to explore the notion that social constructionist approaches to learning, which a building with the hands provides, is a “technique that leverages the potential of the hand-mind dynamic” as historically reported in the extant published literature.Design/methodology/approachThe use of the LEGO® SERIOUS PLAY® (LSP) method in the context of transformative learning in Higher Education was used to drive a Situational analysis with sixteen postgraduate nursing students, from African learning contexts. This methodological approach was used to specifically explore their identity as learners and then to facilitate processes of critical introspection on social constructivist learning opportunities.FindingsStudents’ perceived LSP permitted a deeper level of critical introspection on their transformative learning journeys than alternative approaches, such as written discourse or extended narratives, could have provided. They also perceived that a major benefit of using the LSP method was that it enabled them to understand and articulate their stories more easily than if they verbally reported them first.Research limitations/implicationsThe sampling the authors used was purposive and reflective of the Nigerian background of our research participants, who study at the University of Sunderland.Practical implicationsLSP was perceived as an effective vehicle for the facilitation of reflection and self-awareness, which consequently contribute to students’ capacities to function at a metacognitive level. This has the potential to contribute to authentic transformative learning. Academic learning at postgraduate level hinged on the capacity of students to develop a pragmatic and working knowledge of what acknowledging their epistemic cognition entailed.Originality/valueThe methodological approach implemented in this paper provides a unique means of harnessing a now common gamification technique in pedagogic practice.
Evaluating privacy impact assessment methods: guidelines and best practice
Authors: Vemou Konstantina , Karyda Maria 
Published: September 2019

Purpose
This paper aims to practically guide privacy impact assessment (PIA) implementation by proposing a PIA process incorporating best practices from existing PIA guidelines and privacy research.

Design/methodology/approach
This paper critically reviews and assesses generic PIA methods proposed by related research, data protection authorities and standard’s organizations, to identify best practices and practically support PIA practitioners. To address identified gaps, best practices from privacy literature are proposed.

Findings
This paper proposes a PIA process based on best practices, as well as an evaluation framework for existing PIA guidelines, focusing on practical support to PIA practitioners.

Practical implications
The proposed PIA process facilitates PIA practitioners in organizing and implementing PIA projects. This paper also provides an evaluation framework, comprising a comprehensive set of 17 criteria, for PIA practitioners to assess whether PIA methods/guidelines can adequately support requirements of their PIA projects (e.g. special legal framework and needs for PIA project organization guidance).

Originality/value
This research extends PIA guidelines (e.g. ISO 29134) by providing comprehensive and practical guidance to PIA practitioners. The proposed PIA process is based on best practices identified from evaluation of nine commonly used PIA methods, enriched with guidelines from privacy literature, to accommodate gaps and support tasks that were found to be inadequately described or lacking practical guidance.
A look at the dynamics of personal growth and self-employment exit
Authors: Beutell Nicholas J. , Alstete Jeffrey W. , Schneer Joy A. , Hutt Camille 
Published: September 2019

Purpose
The purpose of this paper is to test a model predicting self-employment (SE) personal growth (learning opportunities and creativity) and SE exit intentions (exiting to work for someone else and exit likelihood) based on the job demands-resources model.

Design/methodology/approach
SEM was used to examine SE demands and resources, strain, and engagement predicting growth, exit intentions, job satisfaction, and life satisfaction. SE type (owners with employees and independent owners without employees) was a moderator variable. Data were analyzed from a national probability sample (n=464 self-employed respondents for whom SE was their primary work involvement), the National Study of the Changing Workforce.

Findings
Overall support for the model was found. Work–family conflict (demand) and work–family synergy (resource) had the strongest relationships with strain and engagement. Strain was positively related to both growth and exit intentions while engagement was inversely related to exit intentions but positively related to growth. The model was significantly different for business owners and independently self-employed.

Practical implications
These results provide guidance to researchers and educators regarding the challenges of self- employment engagement and strain with implications for selecting business types that minimize exit likelihood while maximizing work engagement and personal growth potential.

Originality/value
This study breaks new ground by testing a structural model of engagement and growth for self-employed individuals while also investigating two types of exit intentions. The authors report findings for growth and exit decisions that have received scant attention in the literature to date. Type of SE was a significant variable.
A stakeholder-based risk assessment and intervention framework for international construction projects
Authors: Wang Tao , Gao Shangde , Liao Pinchao , Ganbat Tsenguun , Chen Junhua 
Published: September 2019

PurposeThe purpose of this paper is to construct a two-stage risk management framework for international construction projects based on the meta-network analysis (MNA) approach. A plethora of international construction studies seems to assume risks as independent and therefore, risk intervention strategies are usually critiqued as ineffective.Design/methodology/approachIn the risk assessment stage, a multi-tiered risk network structure was developed with the project objectives, risk events, risk factors and stakeholders, and critical risk factors were selected based on a series of calculations. In the risk intervention stage, targeted risk intervention strategies were proposed for stakeholders based on the results of the first stage. A highway construction project in Eastern Europe was selected as a case study.FindingsThe results showed that 17 risk factors in three categories – external, stakeholder-related and internal – are critical, and the project manager, construction management department, supplier and contract department are the most critical stakeholders that affect the entire project performance. Based on the critical risk factors and project stakeholders, targeted risk intervention strategies were proposed. The risk assessment results of MNA were found to be more reliable and consistent with the project conditions than the risk matrix method; the risk intervention strategies of MNA can effectively address project objectives.Originality/valueThis study modeled risk priorities based on risk associations and put forward a new method for risk management, supplementing the body of knowledge of international construction. The results of this study are of critical importance in management practices.