bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enigmatic incongruence between mtDNA and nDNA revealed by multi-locus phylogenomic analyses in freshwater snails
Authors: Hirano Takahiro , Saito Takumi , Tsunamoto Yoshihiro , Koseki Joichiro , Ye Bin , Do Van Tu , Miura Osamu , Suyama Yoshihisa , Chiba Satoshi 
Published: April 2019

AbstractPhylogenetic incongruence has frequently been encountered among different molecular markers. Recent progress in molecular phylogenomics has provided detailed and important information for evolutionary biology and taxonomy. Here we focused on the freshwater viviparid snails (Cipangopaludina chinensis chinensis and C. c. laeta) of East Asia. We conducted phylogenetic analyses and divergence time estimation using two mitochondrial markers. We also performed population genetic analyses using genome-wide SNPs. We investigated how and which phylogenetic patterns reflect shell morphology. The results showed these two species could be separated into four major mitochondrial clades, whereas the nuclear clusters supported two groups. The phylogenetic patterns of both mtDNA and nDNA largely reflected the geographical distribution. Shell morphology reflected the phylogenetic clusters based on nDNA. The findings also showed these two species diversified in the Pliocene to early Pleistocene era, and occurred introgressive hybridisation. The results also raise the taxonomic issue of the two species.
DNA insecticide developed from the Lymantria dispar 5.8S ribosomal RNA gene provides a novel biotechnology for plant protection
Authors: Oberemok Volodymyr V. , Laikova Kateryna V. , Gal’chinsky Nikita V. , Useinov Refat Z. , Novikov Ilya A. , Temirova Zenure Z. , Shumskykh Maksym N. , Krasnodubets Alisa M. , Repetskaya Anna I. , Dyadichev Valeriy V. , Fomochkina Iryna I. , Bessalova Evgenia Y. , Makalish Tatiana P. , Gninenko Yuri I. , Kubyshkin Anatoly V. 
Published: April 2019

AbstractHaving observed how botanicals and other natural compounds are used by nature to control pests in the environment, we began investigating natural polymers, DNA and RNA, as promising tools for insect pest management. Over the last decade, unmodified short antisense DNA oligonucleotides have shown a clear potential for use as insecticides. Our research has concentrated mainly on Lymantria dispar larvae using an antisense oligoRING sequence from its inhibitor-of-apoptosis gene. In this article, we propose a novel biotechnology to protect plants from insect pests using DNA insecticide with improved insecticidal activity based on a new antisense oligoRIBO-11 sequence from the 5.8S ribosomal RNA gene. This investigational oligoRIBO-11 insecticide causes higher mortality among both L. dispar larvae grown in the lab and those collected from the forest; in addition, it is more affordable and faster acting, which makes it a prospective candidate for use in the development of a ready-to-use preparation.
Role of PTA in the prevention of Cu(amyloid-β) induced ROS formation and amyloid-β oligomerisation in the presence of Zn
Authors: Atrián-Blasco Elena , Cerrada Elena , Faller Peter , Laguna Mariano , Hureau Christelle 
Published: April 2019

AbstractMetal-targeting drugs are being widely explored as a possible treatment for Alzheimer's disease, but most of these ligands are developed to coordinate Cu(ii). In a previous communication (E. Atrián-Blasco, E. Cerrada, A. Conte-Daban, D. Testemale, P. Faller, M. Laguna and C. Hureau, Metallomics, 2015, 7, 1229–1232) we showed another strategy where Cu(i) was targeted with the PTA (1,3,5-triaza-7-phosphaadamantane) ligand that is able to target Cu(ii) as well, reduce it and keep it in a safe complexed species. Removal of Cu(ii) from the amyloid-β peptide prevents the stabilization of oligomers and protofibrils and the complexation of Cu(i) also stops the formation of reactive oxygen species. Besides, zinc, which is found in the synaptic cleft at a higher concentration than copper, can hamper the ability of metal-targeting drug candidates, an issue that is still poorly considered and studied. Here we show that PTA fully retains the above described properties even in the presence of zinc, thus fulfilling an additional pre-requisite for its use as a model of Cu(i)-targeting drug candidates in the Alzheimer's disease context.
Refined ramp event characterisation for wind power ramp control using energy storage system
Authors: Liu Wenlong , Gong Yuzhong , Geng Guangchao , Jiang Quanyuan 
Published: April 2019

With the advantages of fast response and bidirectional charge/discharge, an energy storage system (ESS) plays a promising role in wind power ramp control. In this study, an optimisation model based on refined ramp event characterisation is proposed to achieve continuous wind power ramp control using ESS. Firstly, four kinds of ramp scenarios are characterised considering both the wind power ramp event prediction and the charge/discharge state of ESS. State of charge of ESS is managed within its limits during ramp control, based on the classified ramp scenarios. Secondly, for the classified ramp scenarios, an active adjustment strategy is proposed to decide the expected charging/discharging energy of ESS according to the conditions of wind power and ESS. Thus, an appropriate energy storage reserve can be determined for anticipated ramp events. Refined ramp event characterisation is able to achieve better control performance with higher satisfaction of ramp requirement, less wind energy curtailment as well as promising adaptability to different ramp event predictions, wind conditions and changes of ESS parameters. The effectiveness of the proposed method is verified through case studies with real‐world data from a 100 MW wind farm in China.
Der lumbale Bandscheibenvorfall – Spontanverlauf, Therapieoptionen und klinische Ergebnisse
Authors: Schmieder Kirsten 
Published: April 2019

ZusammenfassungIn der Therapie des lumbalen Bandscheibenvorfalls zeigt die auf den jeweiligen Patienten abgestimmte Kombination aus konservativen und unter Umständen operativen Therapiemaßnahmen die besten Ergebnisse. Es herrscht Übereinstimmung darüber, dass bei bestehenden höhergradigen neurologischen Ausfällen, insbesondere beim Kaudasyndrom, eine operative Therapie erfolgen soll. Beim Vorliegen eines reinen Schmerzsyndromes ist die Datenlage zur Auswahl und Abfolge der verschiedenen Therapieoptionen hingegen nicht eindeutig. Hinsichtlich der Anwendung einer bestimmten operativen Technik gibt es keine eindeutige Überlegenheit eines speziellen Verfahrens. Bei intraoperativ festgestellten großen Defekten im Annulus fibrosus bei jüngeren Frauen kann die Implantation eines Annulusverschlusssystems sinnvoll sein. Die Operationsergebnisse nach Operation eines lumbalen Bandscheibenvorfalls sind gut. In der Rezidivsituation kommt es ebenfalls zu einem guten postoperativen Ergebnis, allerdings wird das Niveau des Ersteingriffs nicht erreicht. Der postoperative Verlauf wird zusätzlich richtungsweisend von Kriterien wie dem präoperativen Aktivitätsausmaß, der Dauer der präoperativen Arbeitsunfähigkeit und psychischen Veränderungen bestimmt. Diese müssen im individuellen Patientenmanagement entsprechend Berücksichtigung finden.
AIHA und Hepatitis nach Infektion durch Mykoplasmen: Fallbericht
Authors: Triantafyllou V. , Haftel L. , Endmann M. 
Published: April 2019

ZusammenfassungWir stellen den Fall eines 7-jährigen Mädchens vor, welches mit laborchemischen Hämolysezeichen zur weiteren Abklärung stationär eingewiesen wurde. Das Mädchen war in einem guten Allgemeinzustand und zeigte keine klinischen Ikteruszeichen. Anamnestisch bestanden Husten und eine Blässe seit 3 Monaten. Wir stellten die Diagnose einer Kälteagglutinin-assoziierten hämolytischen Anämie mit passagerer Hepatitis durch Mykoplasmen und behandelten das Kind mit Clarithromycin über zehn Tage.
Medikamentöse Therapie der Hypertonie
Authors: Alscher Mark Dominik 
Published: April 2019

ZUSAMMENFASSUNGEs gibt nicht wenige Patienten, die sowohl eine Hypertonie als auch eine chronische Nierenschädigung haben. Insbesondere mit zunehmendem Alter nehmen die Veränderungen deutlich zu. In Abhängigkeit von der Einschränkung der Nierenfunktion findet sich ein erhöhtes Risiko für Herz-Kreislauf-Erkrankungen. Um auch die Progression günstig zu beeinflussen, ist gerade bei Patienten mit chronischer Nierenerkrankung eine konsequente Blutdruckeinstellung notwendig. Die Therapieprinzipien bestehen i. d. R. in der Gabe eines Angiotensin-Rezeptor-Blockers oder ACE-Hemmers. Kombiniert werden diese mit Kalziumantagonisten, Diuretika und ggf. Spironolacton unter Beachtung des Kaliumhaushaltes. Als allgemeiner Zielblutdruck kann ein Wert von unter 140/90 mmHg angenommen werden. Werte von unter 130/80 mmHg sind insbesondere bei Proteinurie mit hoher Wahrscheinlichkeit empfehlenswert.
Evaluating the Effect of Monitoring through Telephone (Tele-Monitoring) on Self-Care Behaviors and Readmission of Patients with Heart Failure after Discharge
Authors: Negarandeh Reza , Zolfaghari Mitra , Bashi Nazli , Kiarsi Maryam 
Published: April 2019

Background Heart failure is one of the serious cardiovascular diseases, which poses a global pandemic and places a heavy burden on health care systems worldwide. The incidence of this disease in Iran is higher than in other Asian countries. To reduce patients' complications, readmission rates, and health care expenditures, it is necessary to design interventions, which are culturally appropriate and based on community needs.
Methods In 2016, a randomized clinical trial (IRCT2017010731804N1) was initiated to compare tailored tele-monitoring intervention with usual care. In total, 80 patients completed the study after a follow-up period of 8 weeks. The primary end point was heart failure self-care, which was measured using the Iranian version of the European Heart Failure self-care questionnaire. Secondary end points were heart failure related readmission rates.
Results The mean scores for self-care behaviors of the two groups showed significant difference at the baseline (p = 0.045). The results of the analysis of covariance that was used to control the differences in the pretest scores of self-care behaviors showed that the difference between both groups after the intervention was still significant (p < 0.001). The percentage of patients' readmissions in the intervention group (20%) was less than that in the control group (42.2%); however, the results were not statistically significant (p = 0/066).
Conclusion This study showed that tele-monitoring improved self-care behaviors in Iranian patients with heart failure but did not reduce their readmission rates.
Depression and Anxiety among Patients with Atraumatic Lateral Epicondylitis and Ulnar-Sided Wrist Pain
Authors: Pensak Michael J. , Carry Patrick M. , Entin Jacob M. , Lalka Andy , Shourbaji Nader A. , Scott Frank A. 
Published: April 2019

Background Ulnar-sided wrist pain (UWP) and lateral epicondylitis (LE) are common disorders that can be difficult to treat. Depression and anxiety have been shown to modify patient symptoms, disability and pain.
Questions/Purposes The purpose of our study was to quantify the prevalence of depression and anxiety among patients with LE or UWP. A secondary aim was to determine if these patients report higher levels of pain upon presentation and if they are more likely to require occupational therapy.
Patients and Methods A retrospective chart review was conducted, and patients included those with LE or UWP, atraumatic in origin, ages 18 and over, and ongoing use of noninvasive treatment of LE or UWP.
Results Our final analysis included 97 patients of which 57 had LE, 34 had UWP, and 6 had both. The prevalence of a mood disorder was 34.0%. Anxiety and/or depression was more prevalent in patients with LE compared to UWP. The most common medication was alprazolam. Pain scores averaged 1.2 points higher in subjects with a history of a mental health disorder. After adjusting for age and sex, there was no significant association between prevalence of depression and/or anxiety and utilization of physical or occupational therapy.
Conclusions Patients with either LE, UWP or both along with depression and/or anxiety may be less likely to improve with traditional treatments. Future investigations are warranted focusing on the value of a multidisciplinary team consisting of a hand surgeon, behavioral therapist, or psychologist to optimize treatment response.
Level of Evidence This is a Level IV, case series study.
The Role of Human Platelet Preparation for Toll-Like Receptors 2 and 4 Related Platelet Responsiveness
Authors: Koessler Juergen , Niklaus Marius , Weber Katja , Koessler Angela , Kuhn Sabine , Boeck Markus , Kobsar Anna 
Published: April 2019

Background Like immune cells, platelets express the repertoire of toll-like receptors (TLR), among them TLR2 and TLR4, which are important for the recognition of bacterial patterns. Receptor-mediated functional effects in platelets have been investigated, but reliable conclusions are tampered due to heterogeneous study designs with variable platelet preparation methods. This study compares TLR2- and TLR4-dependent platelet responsiveness in platelet-rich plasma (PRP) and in washed platelets (WPs).
Material and Methods Fresh peripheral blood samples from healthy donors served for the preparation of PRP and WP. Basal and agonist-stimulated TLR2 and TLR4 expression levels were evaluated by flow cytometry. Light transmission aggregometry was used to investigate functional effects of TLR2 and TLR4 stimulation with Pam3CSK4 or LPS (lipopolysaccharides from Escherichia coli) as ligands. The capacity of chemokine release was determined by immunoassays.
Results Pam3CSK4 and LPS (in combination with thrombin) were able to induce aggregation in WP, but not in PRP, with threshold concentrations of 15 µg/mL. Basal expression levels of TLR2 and TLR4 were higher in WP than in PRP, increasing several-fold rapidly and persistently upon platelet activation with potent agonists. Pam3CSK4 (15 µg/mL) or LPS led to the submaximal release of RANTES, PF4, PDGF, NAP-2, and sCD40L from WP. In PRP, secretory effects are less pronounced for RANTES, PDGF, or PF4, and not detectable for NAP-2 or sCD40L.
Conclusion The effects mediated by TLR2 and TLR4 stimulation are dependent on platelet preparation, an important issue for experimental designs and for manufacturing of platelet concentrates in transfusion medicine.
Non-renewable groundwater use and groundwater depletion: a review
Authors: Bierkens Marc F P , Wada Yoshihide 
Published: April 2019

Abstract
Population growth, economic development, and dietary changes have drastically increased the demand for food and water. The resulting expansion of irrigated agriculture into semi-arid areas with limited precipitation and surface water has greatly increased the dependence of irrigated crops on groundwater withdrawal. Also, the increasing number of people living in mega-cities without access to clean surface water or piped drinking water has drastically increased urban groundwater use. The result of these trends has been the steady increase of the use of non-renewable groundwater resources and associated high rates of aquifer depletion around the globe. We present a comprehensive review of the state-of-the-art in research on non-renewable groundwater use and groundwater depletion. We start with a section defining the concepts of non-renewable groundwater, fossil groundwater and groundwater depletion and place these concepts in a hydrogeological perspective. We pay particular attention to the interaction between groundwater withdrawal, recharge and surface water which is critical to understanding sustainable groundwater withdrawal. We provide an overview of methods that have been used to estimate groundwater depletion, followed by an extensive review of global and regional depletion estimates, the adverse impacts of groundwater depletion and the hydroeconomics of groundwater use. We end this review with an outlook for future research based on main research gaps and challenges identified. This review shows that both the estimates of current depletion rates and the future availability of non-renewable groundwater are highly uncertain and that considerable data and research challenges need to be overcome if we hope to reduce this uncertainty in the near future.
Shear-induced alignment of collagen fibrils using 3D cell printing for corneal stroma tissue engineering
Authors: Kim Hyeonji , Jang Jinah , Park Junshin , Lee Kyoung-Pil , Lee Seunghun , Lee Dong-Mok , Kim Ki Hean , Kim Hong Kyun , Cho Dong-Woo 
Published: April 2019

Abstract
The microenvironments of tissues or organs are complex architectures comprised of structural proteins including collagen. Particularly, the cornea is organized in a lattice pattern of collagen fibrils which play a significant role in its transparency. This paper introduces a transparent bioengineered corneal structure for transplantation. The structure is fabricated by inducing shear stress to a corneal stroma-derived decellularized extracellular matrix bioink based on a 3D cell printing technique. The printed structure recapitulates the native macrostructure of the cornea with aligned collagen fibrils which results in the construction of a highly matured and transparent cornea stroma analog. The level of shear stress, controlled by the various size of the printing nozzle, manipulates the arrangement of the fibrillar structure. With proper parameter selection, the printed cornea exhibits high cellular alignment capability, indicating a tissue-specific structural organization of collagen fibrils. In addition, this structural regulation enhances critical cellular events in the assembly of collagen over time. Interestingly, the collagen fibrils that remodeled along with the printing path create a lattice pattern similar to the structure of native human cornea after 4 weeks in vivo. Taken together, these results establish the possibilities and versatility of fabricating aligned collagen fibrils; this represents significant advances in corneal tissue engineering.
Mechanical and liquid phase exfoliation of cylindrite: a natural van der Waals superlattice with intrinsic magnetic interactions
Authors: Niu Yue , Villalva Julia , Frisenda Riccardo , Sanchez-Santolino Gabriel , Ruiz-González Luisa , Pérez Emilio M , García-Hernández Mar , Burzurí Enrique , Castellanos-Gomez Andres 
Published: April 2019

Abstract
We report the isolation of thin flakes of cylindrite, a naturally occurring van der Waals superlattice, by means of mechanical and liquid phase exfoliation. We find that this material is a heavily doped p-type semiconductor with a narrow gap (<0.85 eV) with intrinsic magnetic interactions that are preserved even in the exfoliated nanosheets. Due to its environmental stability and high electrical conductivity, cylindrite can be an interesting alternative to the existing 2D magnetic materials.
Nano sized Fe–Al oxide mixed with natural maize cob sorbent for lead remediation
Authors: Tsade Hizkeal , Abebe Buzuayehu , Murthy H C Ananda 
Published: April 2019

Abstract
The present work involves the synthesis, characterization and sorption behavior of Fe–Al–MC nanocomposite for the removal of Pb (II) ions from industrial waste water. The sorbent was synthesized by impregnation method. X-ray diffraction (XRD), Energy-dispersive x-ray (EDX) and scanning electron microscopy (SEM) techniques were applied to study the surface structure, compositions and morphology of the materials. Fourier transform infrared technique was used to analyze the effect of surface properties on the adsorption behavior of Pb(II) ions. The optimum effects of the parameters on the adsorption of Pb (II) ions were determined to be, pH = 6, dose = 2 g, contact time = 24 h h and agitation speed = 150 rpm and initial Pb (II) ions 10 ppm. Experimentally, the adsorbed amounts of lead ion tend to decrease with increase in pH. Both Langmuir and Freundlich isotherm models fit the equilibrium data well on the adsorbent. Kinetic data correlated well with the pseudo second order kinetic model. Thermodynamic studies resulted in negative ΔG value indicating the spontaneity of the sorption process. The Fe–Al–MC nanocomposite sorbent showed a sorption capacity of 40.00 mg g−1 at pH 6. Therefore, this adsorbent with large sorption capacity and efficiency is a promising adsorbent for the removal of Pb(II) ions from the wastewater.
Financialisation in context: the case of Italy
Authors: Forges Davanzati Guglielmo , Pacella Andrea , Salento Angelo 
Published: April 2019

AbstractThis paper aims at analysing the causes and effects of increasing financial accumulation by Italian firms, within a radical Institutional theoretical framework. It will be argued that financialisation can be imputed to the fact that the biggest Italian companies aim to increase their profits through speculation after the cycle of class struggle of the 1970s. It will also be shown that financial accumulation has contributed to the reduction of economic growth and to the increase of income inequalities. Since very few research studies have dealt with the Italian case, this paper fills a gap.
Antibiotic susceptibility trend before and after long-term use of selective digestive decontamination: a 16 year ecological study
Authors: Lloréns-Villar Yanire , Tusell Fernando , Canut Andrés , Barrasa Helena , Corral Esther , Martín Alejandro , Rodríguez-Gascón Alicia 
Published: April 2019

Abstract

Objectives
The aim of this study was to compare antimicrobial susceptibility rates in a Spanish ICU before and after the introduction of selective digestive decontamination (SDD) and also to compare these with susceptibility data from other Spanish ICUs without SDD.

Methods
We performed a retrospective study in the ICU of the University Hospital of Alava, where SDD was implemented in 2002. The SDD protocol consisted of a 2% mixture of gentamicin, colistin and amphotericin B applied on the buccal mucosa and a suspension of the same drugs in the gastrointestinal tract; additionally, for the first 3 days, systemic ceftriaxone was administered. From 1998 to 2013 we analysed the susceptibility rates for 48 antimicrobial/organism combinations. Interrupted time series using a linear dynamic model with SDD as an intervention was used. Data from other ICUs were obtained from the ENVIN-HELICS national registry.

Results
Only amoxicillin/clavulanic acid against Escherichia coli and Proteus mirabilis, and a high concentration of gentamicin against Enterococcus faecalis, resulted in a significant decrease in the susceptibility rate after the implementation of SDD, with a drop of 20%, 27% and 32%, respectively. Compared with other Spanish ICUs without SDD, the susceptibility rate was higher in the ICU of our hospital in most cases. When it was lower, differences were <10%, except for a high concentration of streptomycin against Enterococcus faecium, for which the difference was 19%.

Conclusions
No relevant changes in the overall susceptibility rate after the implementation of SDD were detected. Susceptibility rates were not lower than those in the Spanish ICUs without SDD.