bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Pilot Study of Sirolimus in Subjects with Cowden Syndrome or Other Syndromes Characterized by Germline Mutations in PTEN
Authors: Komiya Takefumi , Blumenthal Gideon M. , DeChowdhury Roopa , Fioravanti Susan , Ballas Marc S. , Morris John , Hornyak Thomas J. , Wank Stephen , Hewitt Stephen M. , Morrow Betsy , Memmott Regan M. , Rajan Arun , Dennis Phillip A. 
Published: July 2019

Abstract

Lessons Learned
This is the first human interventional study in patients with Cowden syndrome that is driven by inactivation of germline PTEN gene. Single-agent sirolimus, a mTOR inhibitor, suppressed mTOR signaling in surrogate human tissues without significant toxicity.

Background
Cowden syndrome is characterized by inactivating germline PTEN mutations, which can lead to activation of the PI3K-Akt-mTOR pathway.

Methods
Adult subjects with germline PTEN mutation who met international diagnostic criteria for Cowden syndrome and who had Eastern Cooperative Oncology Group (ECOG) performance status 0–2 and adequate organ function were enrolled. Subjects were treated with a 56-day course of daily oral sirolimus. In addition to symptom assessment and physical examination, dermatologic, endoscopic, neurologic (cerebellar), and radiographic assessments were conducted. Inhibition of the mTOR pathway in benign skin and gastrointestinal (GI) lesion was assessed by immunohistochemistry.

Results
A total of 18 patients and 16 families were enrolled. PTEN mutations were located at exons 1–8. Regression of skin and GI lesions was observed by dermoscopy or endoscopy. Neurological evaluation showed improvement in cerebellar function score at 1 month. Immunohistochemistry (IHC) analysis in skin and GI benign lesions showed a decrease in the ratio of phosphorylated (p)S6 to total S6 in response to sirolimus. Ratios of pS6K to total S6 at days 14 and 56 were significantly lower than at baseline (p = .0026, p = .00391, respectively). A 56-day course of sirolimus was well tolerated.

Conclusion
A 56-day course of sirolimus was well tolerated in subjects with Cowden syndrome and was associated with some evidence of improvement in symptoms, skin and GI lesions, cerebellar function, and decreased mTOR signaling.
Оптика медико-демографических процессов в контексте устойчивого развития арктического макрорегиона (на примере Архангельской области)
Authors: Ревич Борис Александрович , Харькова Татьяна Леонидовна , Кваша Екатерина Александровна 
Published: July 2019

Сохранение и укрепление здоровья населения обозначены как приоритеты развития в Государственной программе «Социально-экономическое развитие Арктической зоны Российской Федерации на период до 2020 года». В статье анализируются тренды ожидаемой продолжительности жизни, смертности от основных причин смерти и младенческой смертности в одном из наиболее крупных по числу жителей регионов Арктической зоны Российской Федерации – части Арктического макрорегиона Архангельской области. Рассматривается дифференциация смертности от основных групп причин смерти, включая болезни системы кровообращения (БСК), новообразования, внешние причины смерти, болезни органов дыхания и пищеварения, инфекционные заболевания и некоторые другие причины в гг. Архангельске, Северодвинске, других городах области и в сельской местности. Анализ основан на стандартизованных коэффициентах смертности (СКС) по причинам смерти.
Уровень смертности в Архангельской области выше среднероссийских показателей, особенно у мужчин. Наиболее низкая смертность отмечается в Архангельске и Северодвинске, несколько выше – в средних и малых городах и значительно выше – в сельской местности.
Структура причин смерти во всех группах поселений более чем на 80% определяется БСК, новообразованиями и внешними причинами, но при этом везде отмечается небольшое снижение доли БСК и внешних причин и более значительный рост доли новообразований. Архангельск характеризуется более высоким уровнем СКС от новообразований. Смертность от внешних причин отличается значительной дифференциацией по группам поселений. Однако СКС от случайных отравлений алкоголем во всех группах находится на одном уровне.
IMPLEMENTATION OF THE STATE AGRARIAN PROGRAMME IN THE USSR OF THE SECOND HALF OF THE 1920s AND THE BEGINNING OF THE 1930s
Authors: Melnikova T. A. 
Published: July 2019

The article presents the review of realization of the state agrarian programme of the Soviet state in the second half of the 1920s and the beginning of the 1930s. The change of labour relationship in agriculture,the period of the new economic policy have been analyzed in detail. The process of agrarian reforms in the country aimed at transformation of peasant community into a part of a state system as well as state policy concerning villages have been considered.
Three port versus four port laparoscopic cholecystectomy: a prospective comparative clinical study
Authors: Bari Shams Ul , Islam Faheem Ul , Rather Ajaz A. , Malik Ajaz A. 
Published: July 2019

Background: Although, traditional laparoscopic cholecystectomy is performed using four-port technique, various modifications were made to further enhance the advantages of laparoscopic cholecystectomy. Aim of the study is to compare the results of three-port and four-port laparoscopic cholecystectomy at single center in terms of technical feasibility, safety of the procedure, operative time, intra-operative complications, postoperative pain and post-operative analgesia requirementMethods: It was a prospective comparative study conducted in the department of surgery Skims Medical college Srinagar, India from July 2015 to March 2017. The study was performed on all adult patients with ultrasound documented cholelithiasis and gall bladder Polyposis. The total number of patients studied was 100 which were divided into two groups of 50 each.Results: The average operative time in three port group was 29.2 minutes (range, 15-37) compared to 30.66 minutes (range, 15-42) in four port group, which was statistically insignificant. The final visual analog scores for pain in the postoperative period was 2.30 vs 2.86 in three port and four port group respectively, with a P value=0.008, which was statistically significant.Conclusions: The three-port technique is as safe as the standard four-port technique and can be a viable alternative to four port cholecystectomy with an advantage of less pain and less analgesic requirement and better cosmetic results.
The motor activity in acute cerebral stroke based on the Performance Oriented Mobility Assessment test modified by M. T inetti (1986), functional stress tests and screening assessment of visual-spatial disorders
Authors: Alekhin A. N. , Sakovsky I. V. , Kemstach V. V. , Pomnikov V. G. , Tarantseva V. M. , Dorofeyeva V. V. 
Published: July 2019

Objective. The article presents the survey data of 80 patients in the acute period of cerebral stroke who were treated in the department of the primary vascular center. Design and methods. Upon recommendations of the motor activity, the patients with acute stroke were screened for the presence of neglect. The choice of motor activity was based on the Performance Oriented Mobility Assessment test in the modification of M. Tinetti (1986) and the results of functional stress tests. Results. There were no complications or falls at the hospital when adequate vegetative support and a high score at Performance Oriented Mobility Assessment in the modification of M. Tinetti (1986) were present, as well as in the absence of visual-spatial inability. The patients with the significant visual-spatial neglect showed higher risk of falls, even with adequate vegetative support and postural stability. Results. We demonstrated the possibility, as well as the prognostic value of the Performance Oriented Mobility Assessment in the modification of M. Tinetti (1986) and functional stress tests along with the assessment of visual-spatial neglect in planning the early motor rehabilitation of patients with cerebral stroke. In addition, we developed an algorithm for the motor rehabilitation planning.
Is anemia a clinical marker of NSAIDs-induced upper gastrointestinal lesions in patients with spondyloarthritis?
Authors: Safarova K. N. , Dorogoykina K. D. , Rebrov A. P. 
Published: July 2019

Background: Anemia is a frequent comorbid conditions in patients with spondyloarthritis (SpA). Its development is associated with the activity of the underlying disorder, and in some cases can be a complication of medical treatment. Investigation into the particulars of the anemia, its prevalence in SpA patients is of considerable interest due to the long-term inflammatory process, on the one hand, and high frequency of non-steroidal anti-inflammatory drugs (NSAIDs) usage, on the other.Aim: To assess the incidence of NSAID-induced lesions of upper gastrointestinal tract in patients with SpA and anemia.Materials and methods: This cross-sectional observational study included 107 patients with proven SpA (mean age, 44 [36; 53] years; SpA duration, 16 [9; 21] years; men, 61.9%) who were treated in the Department of Rheumatology, Saratov Regional Clinical Hospital, from 2017 to 2018. We assessed their blood cell counts, C-reactive protein levels, iron kinetic parameters (serum iron, transferrin, ferritin, transferrin saturation). Anemia was diagnosed according to the World Health Organization criteria. Two groups were formed depending on the presence of absolute iron deficiency: group 1, 13 patients with anemia of chronic disease (ACD), group 2, 3 patients with iron deficiency anemia (IDA) and 26 patients with ACD + IDA. Esophagogastroduodenoscopy (EGDS) was performed to assess mucous membranes of the upper gastrointestinal tract in patients with anemia.Results: Anemia was found in 42 (39.3%) patients, 38 (90.5%) of them had mild anemia. The increase in the activity of the underlying disorder judged by changes in the laboratory markers of systemic inflammation (erythrocyte sedimentation rate and C-reactive protein levels) significantly correlated with a decrease in hemoglobin concentration (r = -0.5715 and r = -0.3498 respectively, p<0.05). At EGDS, NSAIDs-induced erosions in the esophagus and/or stomach were found detected in 1 (7.7%) patient of the group 1 and in 1 (3.4%) patient of the group 2. A past episode of NSAIDs-induced erosions in the stomach and duodenum was noted in 4 (13.8%) patients of the group 2, whereas at the time of the study no mucous membranes defects were found at EGDS.Conclusion: Anemia was found in more than one third of SpA patients, with 29 of them (69%) having laboratory signs of iron deficiency, but only 1 (3.4%) patient had NSAIDs-induced gastropathy confirmed at EGDS. NSAIDs-associated enteropathy could be the cause of iron deficiency in these patients that would require additional patient examination. The development of iron deficiency can be pathophysiologically associated to prolonged inhibition of alimentary iron absorption against the background of persistent systemic inflammation and depletion of the iron pool in the absence of gastrointestinal blood losses.
Penggunaan Metode SIIT (Stateless IP/ICMP Translation) Dalam Migrasi IPv4 ke IPv6
Authors: Nugroho Kukuh 
Published: July 2019

Pengguna Internet yang semakin bertambah mengakibatkan meningkatnya kebutuhan penggunaan alamat IP. Saat ini alokasi alamat IPv4 versi public sudah tidak bisa menampung kebutuhan pengguna Internet. IP versi 6 (IPv6) sebagai IP versi terbaru yang memiliki daya tampung yang lebih banyak dibandingkan dengan IP versi 4 (IPv4) dapat dijadikan sebagai solusi terhadap masalah berkurangnya atau menipisnya alokasi alamat IPv4 versi public. Perangkat yang sudah terpasang dalam jaringan dan menggunakan alamat IPv4 tidak secara langsung digantikan dengan alamat IPv6. Pada praktiknya, perangkat yang menggunakan IPv6 akan disandingkan dengan perangkat yang sudah menggunakan IPv4. Konsep penggunaan konsep pengalamatan yang berbeda akan menimbulkan masalah dari sisi konektifitas antar perangkat. Oleh karena itu diperlukan sebuah solusi agar jaringan yang sudah menggunakan IPv4 dapat terhubung dengan perangkat yang menggunakan IPv6. Pada penelitian ini akan digunakan salah satu metode dalam menghubungkan antara jaringan IPv4 dan IPv6 yaitu SIIT (Stateless IP/ICMP Translation). Parameter performansi yang dianalisa adalah throughput, packet loss dan delay. Konsep jaringan yang digunakan adalah client-server, dimana pada sisi server akan diaktifkan dua layanan untuk diuji yaitu transfer file dan video streaming.
Silencing of miR-182 is associated with modulation of tumorigenesis through apoptosis induction in an experimental model of colorectal cancer
Authors: Perilli Lisa , Tessarollo Sofia , Albertoni Laura , Curtarello Matteo , Pastò Anna , Brunetti Efrem , Fassan Matteo , Rugge Massimo , Indraccolo Stefano , Amadori Alberto , Bortoluzzi Stefania , Zanovello Paola 
Published: July 2019

Abstract
Background: miR-182-5p (miR-182) is an oncogenic microRNA (miRNA) in different tumor types and one of the most up-regulated miRNA in colorectal cancer (CRC). Although this microRNA is expressed already in early steps of tumor development, its role in driving tumorigenesis is unclear. Methods: The effects of miR-182 silencing on transcriptomic profile were investigated using two CRC cell lines characterized by different in vivo biological behavior, the MICOL-14h-tert cell line (dormant upon transfer into immunodeficient hosts) and its tumorigenic variant, MICOL-14tum. Apoptosis was studied by annexin/PI staining and cleaved Caspase-3/PARP analysis. The effect of miR-182 silencing on the tumorigenic potential was addressed in a xenogeneic model of MICOL-14tum transplant.Results: Endogenous miR-182 expression was higher in MICOL-14tum than in MICOL-14h-tert cells. Interestingly, miR-182 silencing had a strong impact on gene expression profile, and the positive regulation of apoptotic process was one of the most affected pathways. Accordingly, annexin/PI staining and caspase-3/PARP activation demonstrated that miR-182 treatment significantly increased apoptosis, with a prominent effect in MICOL-14tum cells. Moreover, a significant modulation of cell cycle profile was exerted by anti-miR-182 treatment only in MICOL-14tum cells, where a significant increase in the fraction of cells in G0/G1 phases was observed. Accordingly, a significant growth reduction and a less aggressive histological aspect were observed in tumor masses generated by in vivo transfer of anti-miR-182-treated MICOL-14tum cells into immunodeficient hosts. Conclusions: Altogether, these data indicate that increased miR-182 expression may promote cell proliferation, suppress the apoptotic pathway and ultimately confer aggressive traits on CRC cells.
Sero-prevalence of HCV among HIV infected adult patients attending Comprehensive Care Center at Kenyatta National Hospital
Authors: Ukio Kusirye Boniphace , Anzala Omu , Mureithi Marianne W. 
Published: July 2019

Abstract
Objective

The objective of the study was to assess the prevalence of Hepatitis C virus infection and associated risk factors amongst HIV/AIDS adult patients in a sub-Saharan setting.

Results

Two hundred and forty (240) HIV/AIDS patients were recruited from Comprehensive Care Center at KNH.A total of 21 days were spent in the recruitment. The mean age of the participants was 41 years with a standard deviation of 11. The ages ranged between 18 years and 76 years. Majority of the participants were between 30 and 50 years of age. 70% of recruited participants were residing in Nairobi, 16% in Kiambu, 4% in Machakos, 3% in Kajiado and 7% in other counties. 72% of all recruited participants were females, whereas males accounted for 28%. The Prevalence of HCV among Adult HIV/AIDS was 0%. Among risk factors assessed, sexually transmitted infections accounted for 20.4% of all risk factors. None of the participants had a history of intravenous drug use.
Contaminating reactivity of a monoclonal CCAAT/Enhancer Binding Protein β antibody in differentiating myoblasts
Authors: AlSudais Hamood , Wiper-Bergeron Nadine 
Published: July 2019

Abstract
Objective
CCAAT/Enhancer Binding proteins (C/EBPs) are transcription factors involved in the regulation of a variety of cellular processes. We used the Abcam Recombinant Anti-C/EBP beta antibody (E299) to detect C/EBPβ expression during myogenesis. Though the antibody is monoclonal, and the immunogen used is highly specific to C/EBPβ, we identified an intense band at 23 kDa on western blot that did not correspond to any of the known isoforms of C/EBPβ, or family members predicted to cross-react. Absent in myoblast cells overexpressing C/EBPβ, the band was present when C/EBPβ was knocked down, confirming specificity for a protein other than C/EBPβ. The objective of this work was to identify the contaminating reactivity.
Results
We performed immunoprecipitation followed by mass spectrometry to identified myosin light chain 4 (MYL4) as the unknown band, suggesting that the Abcam monoclonal antibody directed against C/EBPβ is not pure, but contains a contaminating antibody against MYL4. Caution should be used when working in cells lines that express MYL4 to not confound the detection of MYL4 with that of the C/EBPβ LIP isoform.
The predictive value of diaphragm ultrasound for weaning outcomes in critically ill children
Authors: Xue Yang , Zhang Zhen , Sheng Chu-Qiao , Li Yu-Mei , Jia Fei-Yong 
Published: July 2019

Abstract
Background: Multiple studies have shown that diaphragmatic ultrasound can better predict the outcome of weaning in adults. However, there are few studies focusing on children, leading to a lack of sufficient clinical evidence for the application of diaphragmatic ultrasound in children. The purpose of this study was to investigate the predictive value of diaphragm ultrasound for weaning outcomes in critically ill children.
Methods: Study included 50 cases whose mechanical ventilation(MV) time > 48 h, and all eligibles were divided into either the weaning success group (n = 39) or the weaning failure group (n = 11). Diaphragm thickness and diaphragmatic excursion (DE) were measured in the zone of apposition. The maximum inspiratory pressure (PImax) was also recorded.
Results: The ventilatory treatment time (P = 0.002) and length of PICU stay (P = 0.013) in the weaning failure group was longer than the success group. Cut-off values of diaphragmatic measures associated with successful weaning were ≥ 21% for DTF with a sensitivity of 0.82, and a specificity of 0.81, whereas it was ≥ 8.40 mm for DE with a sensitivity of 0.62, and a specificity of 0.91. The linear correlation analysis showed that DTF had no significant correlation with PImax in children (P = 0.31).
Conclusions: Diaphragm ultrasound has great value in predicting the weaning outcome of critically ill children. DTF and DE presented better performance than other diaphragmatic parameters. However, PImax has limited value in terms of reflecting the inspiratory muscle function of children with MV.
Trial Registration: The trial ‘Early rehabilitation intervention for critically ill children’ has been registered at http://www.chictr.org.cn/showproj.aspx?proj=23132. Registration number: ChiCTR1800020196.
Key words: Paediatric; mechanical ventilation; diaphragm; ultrasound; weaning; PImax
Metagenomic next-generation sequencing for mixed pulmonary infection diagnosis
Authors: Wang Jiahui , Han Yelei , Zhou Yang , Feng Jing 
Published: July 2019

Abstract
Background. Metagenomic next-generation sequencing (mNGS) is emerging as a promising technique for pathogens detection. However, reports on the application of mNGS in mixed pulmonary infection remain scarce.
Methods. From July 2018 to March 2019, 55 cases were enrolled in this retrospective analysis. Cases were classified into mixed pulmonary infection (36 [65.5%]) and non-mixed pulmonary infection (19 [34.5%]) according to primary diagnoses. The performances of mNGS and conventional test on mixed pulmonary infection diagnosis and pathogen identification were compared.
Results.The sensitivity of mNGS in mixed pulmonary infection diagnose was much higher than that of conventional test (97.2％ vs 13.9％;P<0.01) , but the specificity was the opposite (63.2％ vs 94.7％;P=0.07) . The positive predictive value of mNGS was 83.3% (95% CI: 68.0-92.5%) , and the negative predictive value was 92.3% (95% CI: 62.1-99.6%) . A total of 5 (9.1%) cases were identified as mixed pulmonary infection by both conventional tests and mNGS, however, the pathogens identification results were consistent between these two methods in only 1(1.8%) case. In summary, the pathogens detected by mNGS in 3 (5.5%) cases were consistent with those by conventional test, and only 1(1.8%) case was mixed pulmonary infection. According to our data, mNGS had a broader spectrum for pathogen detection than conventional tests. In particular, application of mNGS improved the diagnosis of pulmonary fungal infections. Within the 55 cases, mNGS detected and identified fungi in 31 (56.4%) cases, of which only 10 (18.2%) cases were positive for the same fungi by conventional test. The most common pathogen detected was Human cytomegalovirus in our study, which was identified in 19(34.5%) cases of mixed pulmonary infection. Human cytomegalovirus and Pneumocystis jirovecii, which were detected in 7(12.7%) cases, were the most common co-pathogens in the group of mixed pulmonary infection.
Conclusions. mNGS is a promising technique to detect co-pathogens in mixed pulmonary infection, with potential benefits in speed and sensitivity.
MATERIALIZAÃ‡ÃƒO DO PROJETO Ã‰TICO POLÃTICO PROFISSIONAL E AS CONDIÃ‡Ã•ES DE TRABALHO DE ASSISTENTES SOCIAIS NO INTERIOR DE MINAS GERAIS
Authors: Lopes NoÃªmia de FÃ¡tima Silva , Cortes Ana Paula , Segal Daniele Alves , Andrade Ivani de , Silva Janaina de Oliveira , Afonso JÃ©ssica da Silva , Moraes Leslie de , Abreu LornÃ­dia da Silva , Rodrigues VerÃ´nica da Mata Huebra 
Published: July 2019

O objetivo deste estudo foi analisar de que forma o Projeto Ã‰tico PolÃ­tico do ServiÃ§o Social tem sido materializado por assistentes sociais na regiÃ£o do municÃ­pio de ManhuaÃ§u, MG. A prÃ¡tica do ServiÃ§o Social Ã© uma atividade laboral exercida por profissional habilitado, capaz de trabalhar o conjunto de expressÃµes das desigualdades sociais geradas na sociedade capitalista. Os instrumentos de trabalho do ServiÃ§o Social estÃ£o diretamente associados Ã formaÃ§Ã£o teÃ³rica, tÃ©cnico-operacional e Ã©tico-polÃ­tica. Sua finalidade social estÃ¡ voltada para a intervenÃ§Ã£o naperspectiva de democratizaÃ§Ã£o, autonomia dos sujeitos, acesso aos direitos, e Ã clareza do projeto Ã©tico-polÃ­tico - fundamental para o fortalecimento desta categoria profissional e da classe trabalhadora. Este estudo de carÃ¡ter exploratÃ³rio utilizou-se da pesquisa qualitativa fundamentada no mÃ©todo crÃ­tico dialÃ©tico de Marx. Foram entrevistados profissionais do municÃ­pio de ManhuaÃ§u e suas cidades limÃ­trofes. Pode-se perceber que o Projeto Ã‰tico PolÃ­tico se depara com fatores que dificultam sua materializaÃ§Ã£o diante de condiÃ§Ãµes precÃ¡rias de trabalho. Esta realidade reclama por mudanÃ§as e organizaÃ§Ã£o coletiva do profissional de ServiÃ§o Social.
UM RECORTE SOBRE A DEPRECIAÃ‡ÃƒO DO CAPITAL INTELECTUAL
Authors: Souza JosÃ© Carlos de , Moreira Rosane Aparecida , BrandÃ£o Rock Kleyber Silva 
Published: July 2019

O presente artigo aborda aspectos ligados Ã depreciaÃ§Ã£o do capital intelectual, um recorte da proposta de gestÃ£o do conhecimento. O seu objetivo Ã© descrever e analisar como os gestores, embora interessados em potencializar o valor agregado dos ativos intangÃ­veis, correm o risco deâ€œdepreciÃ¡-loâ€, pois parece que a gestÃ£o das pessoas como ativos quantificÃ¡veis remete a uma visÃ£o reducionista dos indivÃ­duos. Ã‰ que na busca de codificaÃ§Ã£o dos conhecimentos, sem o necessÃ¡rio cuidado e sem fugir Ã espiral do modelo tradicional, Ã© possÃ­vel que as pessoas sepercam em meio aos indicadores de retorno dos investimentos, confundidas com aqueles ativos tangÃ­veis. Foi feita uma abordagem exploratÃ³ria em literatura alusiva ao modelo, buscando analisar o discurso da contabilizaÃ§Ã£o do capital humano, identificando riscos de sua depreciaÃ§Ã£o.