bluuebunny/crossref_metadata_2025_split huggingface dataset:
Struktur Komunitas Mesofauna Tanah dan Kapasitas Infiltrasi Air setelah diberi Perlakuan Biostarter Pengurai Bahan Organik
Authors: Andriani Latifah Fitria , Rahadian Rully , Hadi Mochamad 
Published: January 2019

Research about the biostarter application on the biopori concept through parameter of soil mesofauna community structure and water infiltration capacity was aimed to investigate the effect of biostarter product on soil mesofauna community structure and water infiltration rate at Lubang Resapan Biopori (LRB) aged 30 days and 50 days, also the correlation between mesofauna community structure and soil infiltration capacity. This study uses a single factor completely randomized design, which has 5 treatments including control, EM4, Bioklin, Mig Dec, and Orgadec. The results showed that the addition of biostarter affects soil mesofauna community structure, particularly positive effect on the abundance of Oribatida and Mesostigmata. Products Orgadec was able to increase faster the abundance of mesofauna both at the age of LRB 30 days and 50 days, whereas Mig Dec and EM4 starting to show its influence on the LRB after 50 days. Infiltration rate LRB age of 30 days showed a significant difference in treatment of Orgadec only, whereas at the age of 50 days, the significant difference is indicated in the treatment EM4​​, Mig Dec and Orgadec. Based on the Spearman correlation test, the total number of individuals and number of Oribatida. Both showed significant positive correlation with the infiltration rate. Keywords: biostarter, community structure, soil mesofauna, infiltration rate, LR
Eksplorasi Jamur Alkalotoleran dari Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madura, Jawa Timur
Authors: Lusiana Helga , Rukmi MG Isworo , Raharjo Budi 
Published: January 2019

Mold exploration from limestone hills West Sukolilo village, District Labang, Bangkalan, Madura, East Java, conducted to found the alkalotolerant mold isolates and investigate their amylolytic, proteolytic, and cellulolytic activity. Mold isolation were done using spread plate and dilution method on PDA and CMA at pH 8.0, 9.0, and 10.0. Twenty-seven mold isolates have been found consist of four genus i.e. Aspergillus, Fusarium, Curvularia, and Trichoderma. The result of amylolytic test showed Trichoderma longibrachiatum (IMD-26) have the highest activity at pH 8, while Fusarium sp 2 (IMD-24) showed the highest activity at pH 9. Aspergillus flavus (IMD-7) showed highest proteolytic activity at pH 8, while Hyphomycete (IMD-27) showed the highest activity at pH 9. The highest cellulolytic activity at pH 8 showed by Trichoderma harzianum (IMD-25) and pH 9 by Fusarium sporotrichoides (IMD-20). Keyword : alkalotoleran mold, amylolytic, proteolytic, cellulolytic.
Kloning Gen pcbC dari Penicillium chrysogenum ke dalam Plasmid pPICZA untuk Pengembangan Produksi Penisilin G
Authors: Wiharyani Risma , Hardianto Dudi , Kusumaningrum Hermin Pancasakti , Budiharjo Anto 
Published: January 2019

Availability of drugs in Indonesia is still limited by the high prices of drugs due to on the imported raw materials that reaches 95%. Developing antibiotic raw materials can be achieved by increasing of penicillin G production, which is the raw material for the formation of semisynthetic penicillin derivatives through the production of 6-aminopenisillanic acid (6-APA). One of the important enzyme in the penicillin G biosynthesis is Isopenisilin N Synthase (IPNS) that encodes by pcbC gene on Penicillium chrysogenum. This study aimed to obtain a recombinant of pcbC gene fragments that is inserted into pPICZA plasmid. Amplification of pcbC gene used pcbC-F and pcbC-R primers. The pcbC gene fragment was inserted into pPICZA vector and then transformed into TOP 10 F’. The results showed that the recombinant of the pcbC gene fragment from P. chrysogenum has been obtained. Analysis of DNA sequences using the BLAST program showed that the pcbC gene fragment has high homology (99%) with the pcbC gene from P. chrysogenum Wisconsin 54-1255 and P. chrysogenum AS-P-78 which encodes IPNS Keywords: pcbC Gene, Penicillium chrysogenum, cloning, penicillin G
Potensi Tepung Umbi Dahlia Dan Ekstrak Inulin Dahlia Sebagai Sumber Karbon Dalam Produksi Fruktooligosakarida (FOS) Oleh Khamir Kluyveromyces marxianus DUCC-Y-003
Authors: Yuliana Rida , Kusdiyantini Endang , Izzati Munifatul 
Published: January 2019

Penelitian ini bertujuan untuk mengeksplorasi manfaat umbi dahlia dalam bentuk tepung umbi dan ekstrak inulin sebagai substrat untuk memproduksi FOS. Produksi FOS berlangsung secara mikrobial enzimatis dengan bantuan khamir Kluyveromyces marxianus DUCC-Y-003. Pengkulturan khamir pada media kultur berlangsung selama 60 jam dan dilakukan pengukuran tiap 6 jam. Pengukuran tersebut meliputi pertumbuhan khamir, kadar fruktosa medium dan kadar total gula medium. Penentuan FOS dilakukan dengan cara mengukur nilai derajat polimerisasi (DP). DP merupakan hasil perbandingan antara kadar total gula dengan kadar fruktosa pada media kultur. DP FOS berkisar antara 2-10 dan FOS komersil dengan DP 3-5. Penelitian menggunakan Rancangan Acak Kelompok Faktorial (RAKF). Perlakuan terdiri atas S1D1 (substrat tepung, dosis 1 g), S1D2 (tepung, 3 g), S1D3 (tepung, 5 g), S2D1 (ekstrak inulin, 1 g), S2D2 (ekstrak inulin, 3 g), S2D3 (ekstrak inulin, 5 g). Hasil penelitian menunjukkan bahwa pertumbuhan khamir tertinggi pada perlakuan penggunaan ekstrak inulin dengan dosis 1 gram, dan pertumbuhan terendah pada perlakuan penggunaan tepung dengan dosis 5 gram. Pada masing-masing perlakuan, menghasilkan produk FOS dengan nilai DP yang beragam selama waktu inkubasi 60 jam, hanya perlakuan S2D1 (ekstrak inulin, 1g) yang sudah menghasilkan ketiga jenis FOS komersil dalam waktu inkubasi 60 jam. Pada perlakuan S2D1, produk 1-kestosa dihasilkan saat inkubasi 42 jam, produk nystosa saat 48 jam dan produk fruktofurasylnystosa saat 60 jam. Kesimpulan hasil penelitian adalah perlakuan S2D1 paling efektif dalam menghasilkan FOS selama waktu inkubasi 60 jam, sedangkan perlakuan lain dapat menghasilkan FOS yang sama jika lama waktu inkubasi diperpanjang. Kata kunci : inulin, inulinase, derajat polimerisasi, Kluyveromyces marxianus DUCC-Y-0003
Inventarisasi Jenis Capung (Odonata) Pada Areal Persawahan Di Desa Pundenarum Kecamatan Karangawen Kabupaten Demak
Authors: Rizal Samsul , Hadi Mochamad 
Published: January 2019

Paddy fields is one of the important ecosystem that support human life because here produced rice that is the main food to the human. Besides, paddy field ecosystem also have many diversity of insect, including dragonfly (Odonata). Dragonfly (Odonata) is one of the insect that used to be a predators to the pests in the paddy fields, such as Chilo sp and Nilaparvata lugen. The study on dragonfly was conducted in Pundenarum village, Karangawen, Demak. The objectives of this study is to identify the odonata specieses that lived in paddy field. Inventory of odonata specieses done with field by field method and direct catch using insect net. The result of this study is that 5 specieses of odonata were identified in paddy field, i.e: Orthetrum sabina, Crocothemis servillia, Pantala flavescens, Agriocnemis femina dan Agriocnemis pygmea. The odonata specieses that identified is part of 2 family, i.e: Libellulidae and Coenagrionidae. It is also found that all species is part of the suborder Anisoptera (dragonfly) and Zygoptera (damselflies). Key word : dragonfly, Odonata, inventory, paddy field.
Kualitas Simplisia Tanaman Biofarmaka Curcuma domestica Setelah Proses Pemanasan Pada Suhu Dan Waktu Bervariasi
Authors: Kusumaningrum Hermin Pancasakti , Kusdiyantini Endang , Pujiyanto Sri 
Published: January 2019

Curcuma domestica is one of traditional medicinal plants that is found in Gunungpati Semarang. However the dried product do not achieve optimal standard quality for simplicia in terms of microbial contaminant and in an industrial scale household. Knowledge on how to use sterilization to produce better simplicia and reducing contaminant has not been known by farmers, yet. The purpose of this activity was to obtain the best heating treatment on sterilization of Curcuma simplicia using several temperature under sunlight and oven device. It was also want to show microbial growth after heating at several times and their influence on the quality of simplicia after treatment. The method was conducted by simplicia sterilization of C. domestica using sunlight sterilization for a week and using oven at a temperature of 300C, 400C, 500C and 600C for 1, 3, 6, 9, 12, 24, and 48 hours. The results showed that heating at temperature of 500C for 48 hours obtained the best simplicia, followed by heating at a temperature of 600C for 16-48 hours without contaminants after storing period for 3 months. Key words :Curcuma, sterilization, heat, microbia
Perilaku Vektor Malaria Anopheles farauti Laveran (Diptera: Culicidae) Di Ekosistem Pantai (Kabupaten Biak Numfor) Dan Ekosistem Rawa (Kabupaten Asmat) Propinsi Papua
Authors: Kawulur Hanna , Soesilohadi RC Hidayat , Hadisusanto Suwarno , Trisyono Andi 
Published: January 2019

Biak Numfor and Asmat districts reported as malaria endemic areas in Papua (Papua Global Fund, 2011). Anopheles farauti mosquito is one of the malaria vectors in the region. Malaria control efforts have been made but there are still many cases of malaria. Malaria control will provide maximum results if there is a match between the vector behavior and programs undertaken. The purpose of this study was to determine the bionomics factors (behavioral) of malaria vector An. farauti mosquitoes in coastal ecosystems (Biak Numfor) and swamp (Asmat); namely: (a). density (b) age and (c) blood-seeking behavior. The method used in this study is human landing collection, resting collection and ELISA blood-feed. The results showed that the population density of An. farauti mosquito did not have a positive relationship with the number of malaria patients in both study areas. The activity of An. farauti mosquito on blood-seeking at the coastal and swamp ecosystems lasted all night but mainly at 18:00 to 19:00 hour. The study also showed that blood-seeking activity mostly done outside of the house. Age estimation of An. farauti mosquito population at coastal ecosystems ranging from 16-18 days, while in the swamp ecosystem is 12-14 days. Population density, approximately age and blood-seeking behavior indicates that An. farauti mosquitoes on the coastal and swamp ecosystems have the potential to be an effective malaria vector. Keywords: Malaria, Vector, Behavior
Potensi Cangkang Udang Laut (Penaeus monodon F.) Terhadap Profil Lipid Tikus Putih Hiperlipidemia
Authors: Isdadiyanto Sri 
Published: January 2019

The objective of this study was to analyze the potency of sea shrimp carapace (Penaeus monodon F.) on lipid profiles of Sprague Dawley rats induced by high fat ration. The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes lipid profiles. Lipid profiles were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate. The difference between treatments was statistical analysis by Anava, and continued by Lowest Significant Difference Test to locate the difference. The result showed that high fat diet decreased HDL level, but increased total cholesterol level and LDL level. Sea shrimp carapace increased HDL but decreased total cholesterol level and LDL level in rats by high fat induction. Keywords: Sea shrimp carapace, Sprague Dawley rats, lipid profiles
Profil Kolestrol Darah Tikus Setelah Pemberian Virgin Coconut Oil Dan Minyak Zaitun
Authors: Djaelani Muhammad Anwar 
Published: January 2019

Tingginya kadar kolestrol dalam tubuh menjadi pemicu munculnya berbagai penyakit. Masyarakat berusaha menurunkan kadar kolesterol dalam tubuhnya. Komponen VCO berupa asam lemak jenuh sekitar 90% dan asam lemak tak jenuh sekitar 10%. Asam lemak jenuh VCO didominasi oleh asam laurat. VCO mengandung asam laurat dan asam kaprilat. Keduanya merupakan asam lemak rantai sedang yang biasa disebut Medium Chain Fatty Acid (MCFA). Minyak zaitun atau Olive oil mengandung lemak tak jenuh yang tinggi terutama asam oleat dan polifenol. Polifenol merupakan antioksidan yang dapat mengikat LDL teroksidasi. Penelitian ini bertujuan untuk mengkaji efek asam lemak jenuh yang menggunakan VCO dan efek asam lemak tak jenuh yang menggunakan minyak zaitun terhadap profil kolesterol darah pada tikus putih. Penelitian ini menggunakan Tikus putih jantan sebanyak 25 ekor yang dibagi dalam 5 kelompok perlakuan yaitu P1 adalah kelompok kontrol, P2 kelompok yang diberi VCO 5 mL/kg pakan, P3 kelompok yang diberi VCO 10 mL/kg pakan, P4 kelompok yang diberi minyak zaitun 5 mL/kg pakan dan P5 kelompok yang diberi minyak zaitun 10 mL/kg pakan. Tiap perlakuan diulang sebanyak 5 kali. Perlakuan selama 4 minggu. Variabel yang diamati meliputi kadar Kolesterol total, HDL dan LDL darah tikus. Data yang didapat, dianalisis menggunakan ANOVA. Hasil yang didapat menunjukkan pada perlakuan dengan VCO menunjukkan pengaruh yang tidak signifikan terhadap perubahan ketiga variabel penelitian. Pada perlakuan dengan minyak zaitun kadar HDL tidak terdapat perbedaan yang signifikan antar kelompok perlakuan, kadar Kolesterol total pada kelompok perlakuan menunjukkan penurununan yang tidak signifikan dan kadar LDL pada kelompok perlakuan 10 mL/kg pakan menunjukkan penurununan yang signifikan. Dapat disimpulkan bahwa pemberian VCO tidak berpengaruh pada profil kolestrol darah tikus normolipidemia, dan minyak zaitun berpotensi menimbulkan respon penurunan profil kolesterol tikus pada kadar ≥10 mL/kg pakan. Kata kunci: tikus putih, Kolesterol total, HDL dan LDL, Minyak Zaitun, VCO.
Populasi Penggerek Batang Padi pada Ekosistem Sawah Organik dan Sawah Anorganik
Authors: Hadi Mochamad , Soesilohadi RC Hidayat , Wagiman FX , Soehardjono Yayuk Rahayuningsih 
Published: January 2019

Rice stem borer (RSB ) is a major pest for rice in Central Java in the past ten years as well as previous years . The presence of RSB occurs during the rice growing season with a high intensity , especially in the rainy season . RSB which is the main type, yellow RSB Scirpophaga incertulas Walker and white S. innonata. Walker . Other types are pink Sesamia inferens Walker , striped Chilo suppressalis Walker, headed black C. polychrysus Meyrick , and shiny C. auricilius Dudgeon. Organic farming in Central Java is still not widely applied so it is still very less information available, including information on the fluctuations in species diversity and population abundance of RSB. In this study examined the types and abundance fluctuations keenekaragaman RSB populations in rice field ecosystem organic and inorganic. Research will be conducted for one year (3 times transplanting rice) paddy ecosystems in organic and inorganic Bakalrejo Village, District Susukan, Semarang regency. The results showed that the dominant RSB is yellow S. incertulas. Yellow RSB found in every season paddy rice planting in both organic and inorganic rice. While the pink, shiny and white RSB, occasionally encountered. The RSB encountered seem to prefer the ecosystem tend rice paddy organic than inorganic . Keywords : rice stem borer ( RSB ) , organic rice ecosystem, inorganic rice ecosystem.
Ratio Kadar Ldl/Hdl Tikus Putih Sprague Dawley Hiperlipidemia Setelah Diberi Cangkang Udang Laut (Penaeus monodon F.)
Authors: Isdadiyanto Sri 
Published: January 2019

The objective of this study was to analyze the ratio of LDL/HDL level of Sprague Dawley rats induced by high fat ration after given of sea shrimp carapace (Penaeus monodon F.). The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes ratio of LDL/HDL level. LDL level and HDL level were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate. The difference between treatments was statistical analysis by Anava, and continued by Lowest Significant Difference Test to locate the difference. The result showed that high fat diet increased ratio of LDL/HDL level and Sea shrimp carapace decreased ratio of LDL/HDL level. Keywords: Sea shrimp carapace, Sprague Dawley rats, ratio of LDL/HDL level
Perbedaan Somatometri Itik Tegal, Itik Magelang Dan Itik Pengging
Authors: Wulandari Desi , Sunarno Sunarno , Saraswati Tyas Rini 
Published: January 2019

Local ducks in Indonesia there are several kinds, for example Tegal ducks, Magelang ducks and Pengging duck each having characteristics different somatometri. Research on somatometri ducks that includes body weight, leg length, beak length, wing length, body length, and long-neck ducks important to know somatometri of each duck. This study aimed to analyze the differences somatometri at Tegal ducks, Magelang ducks and Pengging duck, which includes weight, leg length, beak length, wing length, body length, and a long neck. Ducks were used in this study maintained in the Central Breeding and Raising Ducks Work Unit Non Ruminant Livestock Banyubiru, Ambarawa. The study was conducted by measuring somatometri of each six monthold ducks. Duck samples taken from three different types of ducks that Tegal duck, Pengging duck and Magelang duck, of each type of sample taken many ducks as 6 tails. Data were analyzed using analysis of variance (ANOVA) with the differentiating factor is the type of duck. If the data were analyzed contained a real difference, then do a further test using the test Least Significant Differences (LSD) at 95% confidence level. The results showed that there was no significant difference in all parameters of the study. Conclusion of the study three types of ducks will have somatometri not unlike that includes, weight, leg length, beak length, wing length, body length and the length of the neck. It was because give feed and feed same time. Keywords: Local Ducks in Central Java, Somatometri, Tegal ducks, Magelang ducks, Pengging duck
Metode Monitoring Resistensi Populasi Aedes aegypti Dengan Penetapan Konsentrasi Diagnostik
Authors: Sari Vicka Kusuma , Tarwotjo Udi , Hadi Mochamad 
Published: January 2019

Aedes aegypti is the main vector of dengue fever disease. The most effective way to prevent dengue fever is cutting the spreading chain of dengue fever by controlling the vector with using conventional chemical insecticides. The used of insecticide intensively has became the main factor of resistance in Tembalang, then monitoring is needed to determine the change of A. aegypti susceptibility status against the insecticide. The aim of this research is to determine the sensitivity of A. aegypti population from five locations in Tembalang against pyrethroid synthetic insecticide, and to determine the validity of the diagnostic concentration as a method of monitoring resistance A. aegypti population in Tembalang. This research was conducted in Laboratory of Ecology and Biosystematics Department of Biology Faculty of Science and Mathematics, Diponegoro University Semarang. The procedure of this research were: larvae collection (A. aegypti), insect breeding test, sensitivity test including bioassay, determination of diagnostic concentration and validation. The result showed that the susceptibility level of five population was not significantly different since all populations are still susceptible, with the LC50 range between 0,0031-0,0043% and FR range between 1-1,39 (<4). The result of validation test of diagnostic concentration was 0,0038%, valid as a monitoring method of the resistance of A. aegypti population against pyrethroid synthetic insecticide in Tembalang because c2 value of the five population was lower than the c2 table (df = 1; α = 0,05) = 3,84. Keywords : A. aegypti, monitoring resistance, LC50, diagnostic concentration..
Pengaruh Perendaman Akar Bibit Bayam Merah (Alternanthera amoena Voss.) dalam Larutan Na2CuEDTA terhadap Pertumbuhan dan Kandungan Antosianin
Authors: Aini Nabilah , Nurchayati Yulita , Suedy Sri Widodo Agung 
Published: January 2019

Red spinach is one of plants containing anthocyanin pigment which has various functions as food and beverage dye, and play a role in the field of health. Anthocyanin biosynthesis in plants is influenced by environmental factors, one of them are nutrients. One of the nutrients needed by plants and part of the anthocyanin biosynthesis pathway is copper (Cu). Cu can be added in the form of a fertilizer such as Na2CuEDTA. This research aims to study the effect of soaking the red spinach in Na2CuEDTA solution on growth and anthocyanin content, and knowing the optimal Na2CuEDTA concentration for growth and anthocyanin content of red spinach. This research conduct to Complete Random Design of single factor pattern with 5 treatments and 5 replications which are aquades, nutrient solution, nutrient solution+Na2CuEDTA 5 ppm, nutrient solution+Na2CuEDTA 10 ppm, nutrient solution+Na2CuEDTA 20 ppm. Sprouts red spinach that already has 4 leaves soaked in the treatment solution for 10 days, then the plants moved into a soil medium inside the pot for 20 days. The analyzing data which is used is ANOVA continuing with further testing of Duncan Multiple Range Test (DMRT) at the 95% significance level. The result of this research show that soaking of red spinach in Na2CuEDTA solution did not show any significant effect on growth. The growth of red spinach plant is more influenced by nutrient solution while the anthocyanin contents is influenced by nutrients in soil medium. Key words : Red Spinach, Copper, Anthocyanin, Na2CuEDTA
Pengaruh Waktu Fermentasi Teh Kombucha Kadar 50% Terhadap Tebal Dinding Dan Diameter Lumen Arteria Koronaria Tikus Putih
Authors: Isdadiyanto Sri 
Published: January 2019

The objective of this study was to analyze the influences of 50% kombucha tea level on wall thickness and lumen diameter of coronary artery in rats. Sixteen male Rattus norvegicus rats, 2 months of age were used as experimental animals, with treatment kombucha tea that has been fermented for 6, 9 and 12 days at the temperature of 25oC per oral. The research design used to a completely randomized with 4 treatment (for 4 weeks) and 4 replications, i.e., P0 = control, without of kombucha tea, P1 = water + 1,8 ml kombucha tea that has been fermented for 6 days in the morning and afternoon, P2 = water + 1,8 ml kombucha tea that has been fermented for 9 days in morning and afternoon, P3 = water + 1,8 ml kombucha tea that has been fermented for 12 days in morning and afternoon. Variables measured were wall thickness and lumen diameter of coronary artery. Data were analyzed with ANOVA followed by Duncan test at 95% confidence level were performed using SPSS version 10,0. The result showed that kombucha tea did not influences the wall thickness and lumen diameter of coronary artery in rats. Key words: Rattus norvegicus, 50% kombucha tea level, wall thickness, lumen diameter, coronary artery