bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fearless Me!©: A feasibility case series of cognitive behavioral therapy for adolescents with intellectual disability
Authors: Hronis Anastasia , Roberts Rachel , Roberts Lynette , Kneebone Ian 
Published: January 2019

AbstractObjectiveThis study evaluated the feasibility of the Fearless Me! program, an online cognitive behavioral therapy (CBT) program for children with intellectual disability (ID) and anxiety.MethodTwenty‐one adolescents with mild to moderate ID participated in ten sessions of the therapist‐assisted Fearless Me! program, combining face‐to‐face group sessions and an online component. A case series design was adopted to assess anxiety symptoms at baseline, throughout intervention, and postintervention. Feasibility of the measures, intervention, and trial design were considered.ResultsThe measures were appropriate and sensitive to changes in anxiety, whereas the need for attention to factors influencing parent’s completion of them was identified. Reliable Change Index and visual analyses of results indicated reductions in anxiety, particularly for older adolescents with heightened levels of anxiety at baseline.ConclusionsThis is one of the first CBT programs for adolescents with ID, and provides preliminary evidence of adapted CBT as a feasible treatment.
ROS‐ and HIF1α‐dependent IGFBP3 upregulation blocks IGF1 survival signaling and thereby mediates high‐glucose‐induced cardiomyocyte apoptosis
Authors: Huang Yao‐Te , Liu Chung‐Hung , Yang Yao‐Chih , Aneja Ritu , Wen Su‐Ying , Huang Chih‐Yang , Kuo Wei‐Wen 
Published: January 2019

AbstractThe prevalence of chronic hyperglycemia and its complications, imposing a critical burden on the worldwide economy and the global healthcare system, is a pressing issue. Mounting evidence indicates that oxidative stress and hypoxia, two noticeable features of hyperglycemia, play a joint crucial role in mediating cellular apoptosis. However, the underlying detailed molecular mechanism remains elusive. Triggered by the observation that insulin‐like growth factor (IGF1)‐binding protein 3 (IGFBP3) can mediate, in renal cells, high‐glucose‐induced apoptosis by elevating oxidative stress, we wish to, in this study, know whether or not the similar scenario holds in cardiac cells and, if so, to find its relevant molecular key players, thereby dissecting the underlying molecular pathway. Specifically, we used a combination of three different cellular sources (H9c2 cells, diabetic rats, and neonatal rat ventricular cardiomyocytes) as our model systems of study. We made use of Co‐IP assay and western blot analysis in conjunction with loss‐of‐function reasoning, gain‐of‐function logic, and inhibitor treatment as our main analytical tools. As a result, briefly, our main findings are that hyperglycemia can induce cardiac IGFBP3 overexpression and secretion, that high levels of IGFBP3 can sequester IGF1 from IGF1 survival pathway, leading to apoptosis, and that IGFBP3 gene upregulation is hypoxia‐inducible factor (HIF)1α‐dependent and reactive oxygen species dependent. Piecing these findings together allows us to propose the improved molecular regulatory mechanism. In conclusion, we have established the molecular roles of IGFBP3, HIF1, and prolyl hydroxylase domain in connecting oxidative stress with hypoxia and in cellular apoptosis under hyperglycemia.
Circular RNA hsa_circ_0007059 indicates prognosis and influences malignant behavior via AKT/mTOR in oral squamous cell carcinoma
Authors: Su Wen , Wang Yufan , Wang Feng , Zhang Biru , Zhang Hanyu , Shen Yuehong , Yang Hongyu 
Published: January 2019

AbstractOral squamous cell carcinoma (OSCC), the most common oral cancer, damages oral epithelial cells after the accumulation of multiple genetic mutations. Although emerging evidence supports the key role of circular RNAs (circRNAs) in various malignancies, the clinical value and function of circRNAs in OSCC remain unclear. In this study, patients with OSCC (n = 8) and controls (
n = 8) were compared using high‐throughput sequencing and microarray circRNA expression profiles. The circRNA hsa_circ_0007059 was downregulated in OSCC. Subsequently, hsa_circ_0007059 levels in OSCC tissues and cell lines were assessed by quantitative reverse‐transcription chain reaction. Loss‐of‐function and gain‐of‐function experiments were performed to determine whether hsa_circ_0007059 affects malignant behavior in SCC15 and CAL27 cells. Importantly, hsa_circ_0007059 upregulation suppressed cell growth, migration, and invasion, facilitating apoptosis of these cells. Furthermore, nude mouse tumor formation was assessed to validate the tumor‐suppressive role of hsa_circ_0007059 in vivo. Finally, hsa_circ_0007059 was determined to alter cell growth via AKT/mTOR signaling, representing a potential prognostic/therapeutic target for OSCC.
Pharmacokinetics, Safety, and Tolerability of Single‐Dose Intravenous Moxifloxacin in Pediatric Patients: Dose Optimization in a Phase 1 Study
Authors: Stass Heino , Lettieri John , Vanevski Konstantina M. , Willmann Stefan , James Laura P. , Sullivan Janice E. , Arrieta Antonio C. , Bradley John S. 
Published: January 2019

AbstractThe pharmacokinetics, safety, and tolerability of a single dose of moxifloxacin were characterized in 31 pediatric patients already receiving antibiotics for a suspected or proven infection in an open‐label phase 1 study. A dosing strategy for each age cohort (Cohort 1: ≥6 years to ≤14 years; Cohort 2: ≥2 years to <6 years; Cohort 3: >3 month to <2 years) was developed using physiology‐based pharmacokinetic modeling combined with a stepwise dosing scheme to obtain a similar exposure to adults receiving 400 mg of moxifloxacin. Doses, adjusted to body weight and age, were gradually escalated from 5 mg/kg in Cohort 1 to 10 mg/kg in Cohort 3 based on interim analysis of the pharmacokinetic and safety data. Plasma and urine samples before and after the 60‐minute infusion were collected for the analysis of moxifloxacin and its metabolites using a validated high‐pressure liquid chromatography assay with tandem mass spectrometry. Moxifloxacin and metabolite concentrations in plasma were within the ranges observed in adults; however, clearance of all analytes was lower in pediatric patients compared with adults. Population pharmacokinetic analyses using the achieved exposure levels in the 3 age cohorts (with known body weight and clearance) predicted similar efficacy and safety profiles to adults. Moxifloxacin was well tolerated in all pediatric age cohorts. Adverse events related to moxifloxacin were mild or moderate in intensity and showed no correlation with increased weight‐adjusted doses. Our findings guided the selection of age‐appropriate clinical doses for a subsequent phase 3 clinical trial in pediatric patients with complicated intra‐abdominal infections.
Contrast‐enhanced MRI for breast cancer screening
Authors: Mann Ritse M. , Kuhl Christiane K. , Moy Linda 
Published: January 2019

Multiple studies in the first decade of the 21st century have established contrast‐enhanced breast MRI as a screening modality for women with a hereditary or familial increased risk for the development of breast cancer. In recent studies, in women with various risk profiles, the sensitivity ranges between 81% and 100%, which is approximately twice as high as the sensitivity of mammography. The specificity increases in follow‐up rounds to around 97%, with positive predictive values for biopsy in the same range as for mammography. MRI preferentially detects the more aggressive/invasive types of breast cancer, but has a higher sensitivity than mammography for any type of cancer. This performance implies that in women screened with breast MRI, all other examinations must be regarded as supplemental. Mammography may yield ~5% additional cancers, mostly ductal carcinoma in situ, while slightly decreasing specificity and increasing the costs. Ultrasound has no supplemental value when MRI is used. Evidence is mounting that in other groups of women the performance of MRI is likewise superior to more conventional screening techniques. Particularly in women with a personal history of breast cancer, the gain seems to be high, but also in women with a biopsy history of lobular carcinoma in situ and even women at average risk, similar results are reported. Initial outcome studies show that breast MRI detects cancer earlier, which induces a stage‐shift increasing the survival benefit of screening. Cost‐effectiveness is still an issue, particularly for women at lower risk. Since costs of the MRI scan itself are a driving factor, efforts to reduce these costs are essential. The use of abbreviated MRI protocols may enable more widespread use of breast MRI for screening.Level of Evidence: 1Technical Efficacy: Stage 5J. Magn. Reson. Imaging 2019;50:377–390.
Aortic dilatation associates with flow displacement and increased circumferential wall shear stress in patients without aortic stenosis: A prospective clinical study
Authors: Kauhanen S. Petteri , Hedman Marja , Kariniemi Elina , Jaakkola Pekka , Vanninen Ritva , Saari Petri , Liimatainen Timo 
Published: January 2019

BackgroundThe relationship between blood flow characteristics and ascending aortic (AA) dilatation has not been studied in patients with a tricuspid aortic valve (TAV) without aortic stenosis.PurposeTo evaluate whether 4D flow characteristics determined in MRI are related to AA dilatation by comparing dilated AA and nondilated AA subjects with TAV.Study TypeProspective.PopulationTwenty patients with dilated AA and 20 age‐matched patients with nondilated AA.Field Strength/Sequence1.5T/4D flow, 2D flow, and anatomic images.AssessmentAltogether, 16 different 4D flow parameters were assessed in 10 planes in the thoracic aorta. Intra‐ and interobserver reproducibility were analyzed.Statistical TestsIndependent t‐test for normally distributed and the Mann–Whitney test for skewed distributed parameters were used. A paired‐samples t‐test was used to compare 2D and 4D flow parameters. Intraclass correlation coefficient (ICC) was used in intra‐ and interobserver reproducibility analysis.ResultsAortic flow was displaced from the centerline of the aorta in the proximal and tubular planes. Flow displacement (FD) was greatest in the proximal plane of AA and was higher in dilated AA (4.5%, range 3.0–5.8%) than in nondilated AA (2.0%, 1.0–3.0%, P < 0.001). Total wall shear stress (WSS) values were 1.3 ± 0.4 times higher on the displaced side than on the opposite side of the aorta (P < 0.01). The circumferential WSS (WSSC) ratio to total WSS was greater in dilated AA, being 0.48 ± 0.11 vs. 0.32 ± 0.09 in the inner curvature of the proximal AA (P < 0.001) and 0.37 ± 0.11 vs. 0.26 ± 0.07 in the whole aortic ring in the distal AA (P < 0.001). Depending on 4D flow parameters, reproducibility varied from excellent (ICC = 0.923) to very low (ICC = 0.204).Data ConclusionThe present study demonstrates that 4D flow measurements help to visualize the pathological flow patterns related to aortic dilatation. Flow displacement and an increased WSSc/WSS ratio are significantly associated with AA dilatation.Level of Evidence: 2Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;50:136–145.
Multiparameter diffusion‐weighted imaging for characterizing pathological patterns in lupus nephritis patients: A preliminary study
Authors: Zhang Shan , Lin Yanwei , Ge Xin , Liu Guiqin , Zhang Jianjian , Xu Shuaishuai , Wu Guangyu , Chen Sheng , Xu Jianrong , Suo Shiteng 
Published: January 2019

BackgroundMicrostructural changes of lupus nephritis (LN) kidney such as inflammatory cell infiltration or fibrosis could influence water molecular movement or diffusion, which indicates that diffusion‐weighted imaging (DWI) may become a valuable tool in evaluation of this disease.PurposeTo explore whether multiparameter diffusion‐weighted imaging (mDWI) could contribute to characterize pathological patterns in LN patients.Study TypeRetrospective.PopulationTwenty‐two patients with LN.Field Strength/SequenceMulti‐b value DWI was performed with a 3.0 T scanner.AssessmentApparent diffusion coefficient (ADC)m, perfusion‐related diffusion coefficient (Df), molecular diffusion coefficient (Ds), perfusion fraction (f), ADCs, α, ADCk, and mean kurtosis (MK) were calculated by monoexponential, biexponential, stretched‐exponential, and kurtosis models fits, respectively.Statistical TestsIndependent sample t‐test, Pearson analysis and receiver operating characteristic (ROC).ResultsIn the whole group, the activity index (AI) correlated significantly with alpha values in the medulla (rho = –0.54, P = 0.03). The chronicity index (CI) correlated significantly with Ds values in the medulla (rho = –0.61, P = 0.02). No significant association was found between any other diffusion parameter and histologic grade with all P > 0.05. For differentiating proliferative LN (Class III or IV) from Class V, the area under the ROC curve (AUC) of alpha in the medulla was 0.833 (P = 0.023).Data ConclusionmDWI might be used for the characterization of pathological patterns in LN patients.Level of Evidence: 3Technical Efficacy Stage: 2J. Magn. Reson. Imaging 2019;50:1075–1084.
Human metapneumovirus infection: Diagnostic impact of radiologic imaging
Authors: Keske Şiran , Gümüş Terman , Köymen Tamer , Sandıkçı Sunay , Tabak Levet , Ergönül Önder 
Published: January 2019

AbstractBackgroundHuman metapneumovirus (hMPV) is a recently detected virus, which can cause mild to severe respiratory tract infections. Through this study, we aimed to detail the outcomes of hMPV infections.Materials/methodsBetween January 2012 and November 2017, patients who had hMPV detected in nasopharyngeal or bronchoalveolar lavage by molecular respiratory pathogen tests were evaluated. The Food and Drug Administration cleared multiplexed‐polymerase chain reaction system (Idaho Technology, Salt Lake City, UT) was used for diagnosis. Chest radiography (CR) and computed tomography (CT) were evaluated by an expert radiologist.ResultsIn total 100 patients were included, the mean age was 22.9 (0‐87) years, and 50% were male. The hospitalization rate was 52%. Lower respiratory system infection (LRTI) was diagnosed in 44 patients with clinical findings, and in 31 patients out of 44 the radiological findings supported the diagnosis. The LRTI rate was significantly higher in adults than children (66.7%‐32.8%; P = 0.001). In CR, peribronchovascular infiltration (PI) was the most common feature seen in 14 out of 18 patients and was generally bilateral (13 out of 18 patients). In CT imaging, ground‐glass opacity was the most common finding seen in 11 out of 16 patients and nodular consolidation in five patients. Ribavirin was given to four patients, three of whom were severe and required respiratory support. None of the patients died of hMPV infection.ConclusionsThe ground‐glass opacity in CT was similar to other respiratory virus infections, and PI in CR was very common and typical; however, nodular consolidation that may mimic bacterial infection was seen in one‐fourth of CT.
Analysis of genetic and serology of hepatitis A virus infection during and after outbreak in two junior high schools in Surabaya, Indonesia
Authors: Juniastuti  , Wahyuddin Dedy , Nihayatussa'adah  , Amin Mochamad , Yamani Laura Navika , Utsumi Takako , Sustini Florentina , Lusida Maria Inge 
Published: January 2019

AbstractOutbreaks of hepatitis A have occurred in some cities in Indonesia. In Surabaya, the capital city of East Java province, Indonesia, hepatitis A outbreaks have been reported since2013, with a marked increase in the number of cases in 2015. The aim of the present study was to analyze the genetic and serology of acute symptomatic cases (early infection) during a hepatitis A outbreak and asymptomatic cases after the outbreak in two junior high schools in Surabaya in 2015 to 2016. Students with acute symptomatic hepatitis A during the outbreak and other students who were asymptomatic 3 to 4 months after the outbreak were enrolled. Asymptomatic students had no symptoms from the outbreak until they were enrolled. Sera were collected to identify anti‐hepatitis A virus (HAV) IgM (by enzyme‐linked immunosorbent assay) and HAV genetic variations/genotypes (using polymerase chain reaction [PCR]‐sequencing and phylogenetic analysis). A total of 33 (97.1%) out of 34 sera of students with acute symptoms were positive for anti‐HAV IgM and 18% of them were positive by PCR, identified as HAV subgenotype IA. No prominent amino acid variations were observed from reported HAV sequences from Indonesia. Among 38 sera of asymptomatic students, most (55.3%) were positive for anti‐HAV IgM, while none were positive by PCR. In conclusion, HAV‐IA was the only subgenotype identified in acute symptomatic cases during the outbreak. The percentage of HAV‐specific IgM‐positive cases was very high among acute symptomatic students, but that was also high among asymptomatic students, which might contribute as the important source of infection during the outbreak.
Evaluation of a silver‐amplified immunochromatography kit for adenoviral conjunctivitis
Authors: Fujimoto Tsuguto , Hanaoka Nozomu , Konagaya Masami , Kobayashi Masaaki , Nakagawa Hisashi , Hatano Hiroshi , Tsukahara‐Kawamura Tomoko , Uchio Eiichi , Kaneko Hisatoshi 
Published: January 2019

ObjectiveTo compare and evaluate the sensitivity of a newly developed silver‐amplified immunochromatography (SAI) kit with various immunochromatography (IC) kits for adenoviruses based on the detection limit (copies/test).MethodsAn SAI kit and four ophthalmic IC kits were evaluated. The detection limits of the five kits were determined using the limiting dilution method for 15 conjunctivitis‐associated adenoviruses (adenoviruses 1, 2, 3, 4, 5, 7, 8, 11, 37, 53, 54, 56, 64, 81, and 85). The detection limits were presented as numerical values as determined by real‐time polymerase chain reaction (PCR).ResultsThe detection limit of the SAI kit for the adenovirus types ranged from 1.0 × 103–5.0 × 10
4 copies/test (geometric mean, 4.7 × 10
3). SAI had a 10–250‐fold lower detection limit than the four IC kits for all adenoviruses studied. There were also differences in detection limits among the adenovirus types for each kit.DiscussionThe detection limit of the SAI kit was drastically reduced because the silver‐amplification reaction increased the color development sensitivity. The results revealed the high sensitivity of SAI for detecting adenoviruses and suggested its usefulness for conjunctivitis examination.
Novel capsule phase microextraction in combination with high performance liquid chromatography with diode array detection for rapid monitoring of sulfonamide drugs in milk
Authors: Georgiadis Doukas‐Evangelos , Tsalbouris Athanasios , Kabir Abuzar , Furton Kenneth G. , Samanidou Victoria 
Published: January 2019

AbstractCapsule phase microextraction is introduced herein for the first time to determine four sulfonamide residues in milk samples (sulfanilamide, sulfadiazine, sulfamethizole, and sulfathiazole). The technique eloquently integrates filtration and stirring mechanism into the extraction device, as such no filtration of the sample is needed prior to introducing the extraction device into the sample, and when placed on a magnetic stirrer, the device spins itself in order to diffuse the sample, resulting in faster extraction equilibrium. Microextraction capsules consist of three main parts; a magnet, a cellulose fiber substrate coated with high performance sol‐gel hybrid organic‐inorganic sorbent, and a porous membrane. Various encapsulated sol‐gel sorbents were tested in standard solutions prepared in deionized water and milk samples under different operational conditions. Analyte extraction time and elution time, type of sol‐gel sorbent, elution solvent, as well as the ratio of the sorbent to the elution solvent were among the optimized conditions. The protocols that yielded the best absolute recovery rates were subsequently tested in various milk samples. Method validation was performed in terms of linearity, accuracy and precision, reusability and ruggedness using the Youden test. The examined sulfonamides were subsequently analysed by reversed phase high performance liquid chromatography with diode array detection.
Pilot Study of the International Parkinson and Movement Disorder Society‐sponsored Non‐motor Rating Scale (MDS‐NMS)
Authors: Martinez‐Martin Pablo , Schrag Anette , Weintraub Daniel , Rizos Alexandra , Rodriguez‐Blazquez Carmen , Chaudhuri Kallol Ray ,   on behalf of the IPMDS Non Motor PD Study Group
Published: January 2019

ABSTRACTBackgroundNon‐motor symptoms (NMS) are integral to Parkinson's disease (PD) and have a detrimental effect on patients and their caregivers. Clinical quantification has been aided by the development of comprehensive assessments such as the Non‐Motor Symptoms Questionnaire (NMSQuest) and Scale (NMSS). The NMSS has been widely used in clinical studies and trials; however, since its validation in 2007, our understanding of NMS has changed substantially. With the support of the International Parkinson and Movement Disorder Society (IPMDS), after a detailed peer review an initiative to develop an updated version of NMSS, the MDS‐NMS was launched in 2015.ObjectiveThis paper encapsulates the data from the pre‐validation phases carried out under the auspices of the IPMDS Non‐Motor PD Study Group.MethodsItem selection and wording (formatted as a rater‐based tool) were based on the NMSS, literature review, and expert consensus. Neurologists, PD patients, and healthy controls were included in the cognitive pretesting and administration of the preliminary version of the MDS‐NMS. Primary data on acceptability and reliability were obtained.ResultsThe pilot study, carried out in English in the United Kingdom and the United States, demonstrated that the preliminary version of the MDS‐NMS was comprehensive, understandable, and appropriate. Data quality was excellent; moderate floor effect was present in patients for most MDS‐MNS domains, with some components showing weak internal consistency. The results led to additional instrument modifications.ConclusionQualitative and quantitative research results have led to an updated NMSS, the definitive version of the MDS‐NMS, which is currently being validated.