bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein interaction energy landscapes are shaped by functional and also non-functional partners
Authors: Schweke Hugo , Mucchielli Marie-Hélène , Sacquin-Mora Sophie , Bei Wanying , Lopes Anne 
Published: April 2018

AbstractIn the crowded cell, a strong selective pressure operates on the proteome to limit the competition between functional and non-functional protein-protein interactions. We developed an original theoretical framework in order to interrogate how this competition constrains the behavior of proteins with respect to their partners or random encounters. Our theoretical framework relies on a two-dimensional (2D) representation of interaction energy landscapes with 2D energy maps that reflect in a synthetic way the propensity of a protein to interact with another protein. We investigated the propensity of protein surfaces to interact with functional and arbitrary partners and asked whether their interaction propensity is conserved during the evolution. Therefore, we performed several thousands of cross-docking simulations to systematically characterize the whole energy landscapes of 74 proteins interacting with different sets of homologs, corresponding to their functional partner’s family or arbitrary protein families. Then, we systematically compared the energy maps resulting from the docking of a given protein with the different protein families of the dataset. Strikingly, we show that the interaction propensity not only of the binding site but also of the rest of the protein surface is conserved for docking partners belonging to the same protein family. Interestingly, this observation holds for docked proteins corresponding to true but also to arbitrary partners. Our theoretical framework enables the characterization of the energy behavior of a protein in interaction with hundreds of selected partners and opens the way for further developments to study the behavior of proteins in a specific environment.
PCPE2 and SR-BI Partner to Impact Accumulation of Fat in Mice
Authors: Xu Hao , Kaul Sushma , Kallinger Rachel , Thomas Michael J. , Schill Rebecca L. , Sahoo Daisy , Sorci-Thomas Mary G. 
Published: April 2018

ABSTRACTLDL receptor knockout mice (LDLr-/-) were crossed with PCPE2 knockout mice to obtainLdlr-/-,Pcpe2-/-mice. The rationale of these studies was to examine the effects of an extracellular matrix protein, PCPE2, on fat storage in a dyslipidemic mouse model. MaleLdlr-/-,Pcpe2-/-mice were fed a Western diet for 25 weeks and their plasma triglyceride metabolism and triglyceride storage was examined. Interestingly, visceral but not subcutaneous fat pad were smaller in diet-fedLdlr-/-,Pcpe2-/-mice compared to controls. There was no difference in the fatty acid distribution in triglyceride and cholesteryl esters (CE) among the genotypes.Ldlr-/-,Pcpe2-/-mice have higher plasma triglyceride levels and reduced lipoprotein lipase activity. Immunoprecipitation of SR-BI from cell extracts co-precipitated PCPE2 suggesting that PCPE2 and SR-BI are tightly associated. This work also showed that in the absence of PCPE2 SR-BI does not transfer CE from HDL into the cell. These results suggest that HDL, PCPE2, SR-BI, and possibly LPL are associated in an interactome that is required for CE transport into the cell. In the absence of these interactions lipid transport is significantly disrupted.
Profound perturbation of the human metabolome by obesity
Authors: Cirulli Elizabeth T. , Guo Lining , Swisher Christine Leon , Shah Naisha , Huang Lei , Napier Lori A. , Kirkness Ewen F. , Spector Tim D. , Caskey C. Thomas , Thorens Bernard , Venter J. Craig , Telenti Amalio 
Published: April 2018

ABSTRACTObesity is a heterogeneous phenotype that is crudely measured by body mass index (BMI). More precise phenotyping and categorization of risk in large numbers of people with obesity is needed to advance clinical care and drug development. Here, we used non-targeted metabolome analysis and whole genome sequencing to identify metabolic and genetic signatures of obesity. We collected anthropomorphic and metabolic measurements at three timepoints over a median of 13 years in 1,969 adult twins of European ancestry and at a single timepoint in 427 unrelated volunteers. We observe that obesity results in a profound perturbation of the metabolome; nearly a third of the assayed metabolites are associated with changes in BMI. A metabolome signature identifies the healthy obese and also identifies lean individuals with abnormal metabolomes – these groups differ in health outcomes and underlying genetic risk. Because metabolome profiling identifies clinically meaningful heterogeneity in obesity, this approach could help select patients for clinical trials.
Conditional control of fluorescent protein degradation by an auxin-dependent nanobody
Authors: Daniel Katrin , Icha Jaroslav , Horenburg Cindy , Müller Doris , Norden Caren , Mansfeld Jörg 
Published: April 2018

AbstractThe conditional and reversible depletion of proteins by auxin-mediated degradation is a powerful tool to investigate protein functions in cells and whole organisms. However, its wider applications require fusing the auxin-inducible degron (AID) to individual target proteins. Thus, establishing the auxin system for multiple proteins can be challenging. Another approach for directed protein degradation are anti-GFP nanobodies, which can be applied to GFP stock collections that are readily available in different experimental models. Here, we combine the advantages of auxin and nanobody-based degradation technologies creating an AID-nanobody to degrade GFP-tagged proteins at different cellular structures in a conditional and reversible manner in human cells. We demonstrate efficient and reversible inactivation of the anaphase promoting complex/cyclosome (APC/C) and thus provide new means to study the functions of this essential ubiquitin E3 ligase. Further, we establish auxin degradation in a vertebrate model organism by employing AID-nanobodies in zebrafish.
The role of self-efficacy and customer satisfaction in driving loyalty to the mobile shopping application
Authors: Thakur Rakhi 
Published: April 2018

Purpose
The purpose of this paper is to develop and empirically test a model that examines the relationship between post-adoption self-efficacy, satisfaction, and loyalty in the usage of mobile shopping applications.

Design/methodology/approach
A structured questionnaire was used to collect data from respondents who had used mobile shopping applications to make purchases. Data analysis was done using partial least square structural equation modelling.

Findings
The results show that self-efficacy and satisfaction have a positive impact on continuance intention; however, the same may not lead to advocacy. The results also show that some antecedents of self-efficacy and satisfaction at the post-adoption stage differ from the pre-adoption intention stage.

Practical implications
The findings of the study provide a better understanding of the factors likely to influence loyalty among customers using mobile shopping applications. The findings also provide valuable insights into the factors that e-retailers need to focus to build self-efficacy among their customers using mobile interface.

Originality/value
The contribution of the paper lies in eliciting the differences between customer choice model at the pre-adoption and post-adoption stage for mobile shopping. Furthermore, the study demonstrated the role of a cognitive factor of self-efficacy in loyalty at the post-adoption stage that is pre-dominantly researched with affective factor of satisfaction.
The proxies conundrum
Authors: Tashfeen Rubeena , Azhar Tashfeen Mahmood 
Published: April 2018

Purpose
No systematic models are being used in empirical research that provide assurance for the choice of proxies that are being used. The purpose of this paper is to examine the validity of the proxies being used in empirical research, and as a case study, it focuses on the area of financial derivatives.

Design/methodology/approach
First, the authors review results of proxies from the financial derivatives literature and follow with empirical tests to confirm the findings from the review.

Findings
The review shows that proxies provide mixed results. The findings are further supported by the results from empirical tests. It suggests that measures used in the studies related to financial derivatives theory may need to be refined and highlights that no solid bases or tests have been developed for the proxies used to measure the constructs.

Research limitations/implications
As individual proxies are examined across studies, a meta-regression analysis cannot be used, and there is no other available model to capture this type of examination. The approach adopted has some limitations but provides a basis for examining the reasonableness of proxies as measures of constructs.

Originality/value
This is the first study that attempts to examine the strength of proxies in capturing related constructs. The methodology is unique to a review of past studies in financial derivatives. It supports the need for developing more rigorous models/bases for the measures being used, and this is an area that has been ignored in empirical research.
Movement patterns and herd dynamics among South African giraffes (Giraffa camelopardalis giraffa)
Authors: Deacon Francois , Bercovitch Fred B. 
Published: April 2018

AbstractGiraffes reside in a fission–fusion social system, with sex, age proximity, kinship and home range overlap accounting for some of the variance in herd composition, while season, sex, age and time of day influence diet, home range size and distance travelled. To increase our knowledge of habitat use and fission–fusion herd dynamics, we placed GPS devices on eight adult female South African giraffes (Giraffa camelopardalis giraffa) living in the Khamab Kalahari Nature Reserve (South Africa). We tested four predictions about how season, kinship, home range and travel patterns influence habitat use and herd dynamics. Our two key findings were that females with a greater degree of home range overlap were more likely to form herds, but the degree of overlap was independent of the amount of time that they spent together in a herd, and that on the day prior to herd formation, females travelled about twice as far as their daily average and tended to move directly towards their future herd mate. We conclude that habitat use and movement patterns regulating fission–fusion dynamics reflect an interaction of ecological, social and reproductive factors operating in tandem, not independently.
Tell the story: How to write forAmerican Ethnologist
Authors: BESNIER NIKO , MORALES PABLO 
Published: April 2018

ABSTRACTTo write a successful article for a major anthropology journal, authors can employ a number of strategies. The first and most essential is to familiarize themselves with the journal's mission. ForAE, authors must ground their arguments in current debates in the discipline, make a clear contribution to anthropological theory, support this contribution with ethnography, and demonstrate its relevance to contemporary sociocultural problems. Moreover,AEauthors must cite other authors appropriately, cohesively structure the article, and avoid various problems that afflict much of academic writing—such as evasive metadiscourse, inflated diction, serial citation, and unnecessary literature reviews. To maximize the article's online discoverability, authors should judiciously compose their titles and abstracts and carefully choose their accompanying keywords. [writing,composition, publishing,editing,theory,ethnography,American Ethnologist]
Contemporary management of adult‐acquired buried penis
Authors: Jun Min S. , Gallegos Maxx A. , Santucci Richard A. 
Published: April 2018

ObjectivesTo describe our buried penis repair technique that includes penile release, tissue resection, wound closure, and penile reconstruction.Patients and MethodsIn all, 73 patients were treated from 2007 to 2017. Patients can be categorised into five stages: Stage I, involves only a phimotic band; Stage 2, required excision of diseased penile skin with split‐thickness skin grafting (STSG); Stage 3, requires scrotal excision; Stage 4, requires escutcheonectomy; and Stage 5, requires panniculectomy. Successful treatment hinges on adequate excision of diseased skin and de‐bulking followed by replacement of deficient skin with STSG.ResultsIn all, 36 of 73 (49%) patients had Stage 1–3 disease, whilst 37 of 73 (51%) were Stage 4–5. There were complications within the first 30 days in 44 of 73 (60%) patients. In all, 62 of 73 (85%) patients either had no complications or Clavien–Dindo grade I–II complications and nine (12%) had complications beyond 30 days. Only five of 36 (14%) patients with Stage 1–3 disease had complications. One patient developed recurrent phimosis.ConclusionThe buried penis is a challenging surgical entity where conservative treatment will most likely lead to failure. Surgery is the only means for a lasting cure in these patients and should be used as a first‐line treatment. One should expect complications postoperatively, especially within the first 30 days; however, these are mostly limited to Clavien–Dindo grade I–II complications.
Impact of cross‐sex hormone therapy on bone mineral density and body composition in transwomen
Authors: Fighera Tayane Muniz , da Silva Eliane , Lindenau Juliana Dal‐Ri , Spritzer Poli Mara 
Published: April 2018

SummaryObjectiveCross‐sex hormone therapy (CSHT) has been associated with changes in bone and lean/fat mass. This study assessed bone mineral density (BMD), appendicular lean mass (ALM), and total fat mass in transwomen undergoing CSHT.Patients and DesignWe evaluated 142 transwomen (mean age: 33.7 ± 10.3 years; BMI: 25.4 ± 4.6; 86.6% with previous CSHT) during the first 3 months of regular oestrogen treatment (with or without anti‐androgens). A reference group including 22 men and 17 cis women was also studied.MeasurementsClinical and hormonal evaluation and dual‐energy X‐ray absorptiometry (DXA).ResultsBone mineral density was similar in trans and reference women, and lower at all sites in transwomen vs men. Low bone mass for age was observed in 18% of transwomen at baseline vs none of the reference women or men. Appendicular lean mass and total fat mass were positively correlated with L1‐L4 BMD, explaining 14.9% of the observed variation in lumbar spine BMD and 20.6% of the variation in total femur BMD. Appendicular lean mass was similar in trans and reference women, and lower in transwomen vs men. Total fat mass was lower in trans vs reference women. Densitometry was repeated after a mean of 31.3 ± 6.5 months in 46 transwomen. There was a significant increase in total fat mass and a significant decrease in ALM. Bone mineral density remained stable over time.ConclusionsThe fairly high prevalence of low bone mass in this sample of transwomen from southern Brazil seems to be related to lower ALM. Non‐pharmacological lifestyle‐related strategies for preventing bone loss could be beneficial for transgender women receiving long‐term CSHT.
Too Much of a Good Thing: How Novelty Biases and Vocabulary Influence Known and Novel Referent Selection in 18‐Month‐Old Children and Associative Learning Models
Authors: Kucker Sarah C. , McMurray Bob , Samuelson Larissa K. 
Published: April 2018

AbstractIdentifying the referent of novel words is a complex process that young children do with relative ease. When given multiple objects along with a novel word, children select the most novel item, sometimes retaining the word‐referent link. Prior work is inconsistent, however, on the role of object novelty. Two experiments examine 18‐month‐old children's performance on referent selection and retention with novel and known words. The results reveal a pervasive novelty bias on referent selection with both known and novel names and, across individual children, a negative correlation between attention to novelty and retention of new word‐referent links. A computational model examines possible sources of the bias, suggesting novelty supports in‐the‐moment behavior but not retention. Together, results suggest that when lexical knowledge is weak, attention to novelty drives behavior, but alone does not sustain learning. Importantly, the results demonstrate that word learning may be driven, in part, by low‐level perceptual processes.
The role of accounting fundamentals and other information in analyst forecast errors
Authors: Monte‐Mor Danilo S. , Galdi Fernando C. , Costa Cristiano M. 
Published: April 2018

AbstractIn this paper, we study analyst forecast errors in the United States, and decompose these errors into two different sources: accounting fundamentals and other information. Using data from 1983 to 2012, our results lead to two conclusions. First, using the decomposition approach, we show that on average, the component of analyst forecast errors based on ‘accounting information’ is optimistic; however, the component of analyst forecast errors based on ‘other information’ is pessimistic. Second, although occasionally analysts make forecasts with small errors, the decomposition of such errors provide, on average, larger (positive) accounting errors, and larger (negative) other information errors. In this case, our results suggest that analysts' luck occasionally surpasses their skills.
The Influence of Administrative Traditions and Governance on Private Involvement in Urban Climate Change Adaptation
Authors: Klein Johannes , Juhola Sirkku 
Published: April 2018

AbstractThis qualitative study examines how administrative traditions and governance in two cities, New York City and Helsinki, have affected the interplay of public administration, the private sector, and citizens in climate change adaptation. The results show that both cities aim for an increased state‐market‐civil society interaction. However, their efforts have different points of departure and follow different trajectories. New York City's administration is within the Anglo‐American tradition of state‐market dualism, while the state‐market‐civil society interaction for adaptation is coordinated by newly created administrative organizations. This interaction has to be accommodated to the strong reliance on market mechanisms. Helsinki's administration has its roots in the welfare state administration with a strong role of the public administration. Increased state‐market‐civil society interaction results in a reduced influence of the public sector and the reliance on ad hoc projects and informal modes of cross‐sectoral cooperation.
Implicit Statistical Learning: A Tale of Two Literatures
Authors: Christiansen Morten H. 
Published: April 2018

AbstractImplicit learning and statistical learning are two contemporary approaches to the long‐standing question in psychology and cognitive science of how organisms pick up on patterned regularities in their environment. Although both approaches focus on the learner's ability to use distributional properties to discover patterns in the input, the relevant research has largely been published in separate literatures and with surprisingly little cross‐pollination between them. This has resulted in apparently opposing perspectives on the computations involved in learning, pitting chunk‐based learning against probabilistic learning. In this paper, I trace the nearly century‐long historical pedigree of the two approaches to learning and argue for their integration under the heading of “implicit statistical learning.” Building on basic insights from the memory literature, I sketch a framework for statistically based chunking that aims to provide a unified basis for understanding implicit statistical learning.
Native language and acquired language as determinants of product‐level trade
Authors: Egger Peter H. , Toubal Farid 
Published: April 2018

AbstractThe present paper estimates response parameters of bilateral export flows at the disaggregated Harmonised System 6‐digit level to two fractional variables reflecting the language commonality between exporting and importing countries common native language versus common acquired language. In particular, the response to common acquired language is shown to vary systematically with the price (and trade) elasticity of demand across products and with the degree of product differentiation and competition across products. These findings support the notion that there is a relatively important role to play for foreign‐language acquisition in stimulating cross‐border trade and international integration, which are known to induce (average) consumer gains.
Wireless bipolar electrosurgical device by using power energy transfer
Authors: Alzaidi Ali Idham , Yahya Azli , Tian Swee Tan , Idris Norhalimah 
Published: April 2018

This paper presents to develop a system of wireless power transfer for medical applications.Many surgeons use the bipolar electrosurgery system that runs on a battery for a different kind of surgery. This venture is intended to control a rechargeable cordless remote which is used in operation room/theatre. This venture is framed out from Alternating Current(AC) of 220 Volt(V) 50 Hz to 12V Direct Current(DC) then converted to 12V AC at 20 kHz in the circuit. DC battery is provided in an electrode of the 12 V bipolar electrosurgery system and the arrangement for charging battery is also prudently/properly made. The new design is an improvement based on specialists' complain regarding the visibility of wire/eletrical line of standard electrocautery instrument Theose wire always put them at risk of being tangled on the searing instrument which can burn the hand. Not only that, it also restrict their hands movement from performing surgery. The electrical wire is a weakness/disadvantage as it requires an observation of eletrical wire from the hand position to guarantee that it stays sterile amid surgical operations.
RNase III Processing of rRNA in the Lyme Disease Spirochete Borrelia burgdorferi
Authors: Anacker Melissa L. , Drecktrah Dan , LeCoultre Richard D. , Lybecker Meghan , Samuels D. Scott 
Published: April 2018

ABSTRACT

The rRNA genes of
Borrelia
(
Borreliella
)
burgdorferi
are unusually organized; the spirochete has a single 16S rRNA gene that is more than 3 kb from a tandem pair of 23S-5S rRNA operons. We generated an
rnc
null mutant in
B. burgdorferi
that exhibits a pleiotropic phenotype, including decreased growth rate and increased cell length. Here, we demonstrate that endoribonuclease III (RNase III) is, as expected, involved in processing the 23S rRNA in
B. burgdorferi
. The 5′ and 3′ ends of the three rRNAs were determined in the wild type and
rnc
Bb
mutants; the results suggest that RNase III in
B. burgdorferi
is required for the full maturation of the 23S rRNA but not for the 5S rRNA nor, curiously, for the 16S rRNA.

IMPORTANCE
Lyme disease, the most common tick-borne zoonosis in the Northern Hemisphere, is caused by the bacterium
Borrelia
(
Borreliella
)
burgdorferi
, a member of the deeply branching spirochete phylum.
B. burgdorferi
carries a limited suite of ribonucleases, enzymes that cleave RNA during processing and degradation. Several ribonucleases, including RNase III, are involved in the production of ribosomes, which catalyze translation and are a major target of antibiotics. This is the first study to dissect the role of an RNase in any spirochete. We demonstrate that an RNase III mutant is viable but has altered processing of rRNA.