bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic significance of lncRNA DANCR expression in human cancers: a systematic review and meta-analysis
Authors: Liu Wei , Wang Qin-Peng , Guo Jia 
Published: March 2019

Abstract
Several studies demonstrated that lncRNA differentiation antagonizing non-protein coding RNA (lncRNA DANCR) expression might have the potential capacity to predict the cancer prognosis; however, definite conclusion has not been obtained. The aim of this meta-analysis was to evaluate the prognostic value of lncRNA DANCR expression in cancers. PubMed, Web of Science, Scopus, and Embase were comprehensively searched for relevant studies. Studies meeting all inclusion standards were included into this meta-analysis. The analysis of overall survival (OS), disease-free survival (DFS), or clinicopathological features was conducted. Total 11 studies containing 1154 cancer patients were analyzed in this meta-analysis. The results showed, compared with low lncRNA DANCR expression, high lncRNA DANCR expression was significantly associated with shorter OS (hazard ratio [HR] = 1.85; 95% CI = 1.52–2.26; P&lt;0.01) and DFS (HR = 1.82; 95% CI = 1.43–2.32; P&lt;0.01) in cancers. Besides, high lncRNA DANCR expression predicted deeper tumor invasion (P&lt;0.01), earlier lymph node metastasis (P&lt;0.01), earlier distant metastasis (P&lt;0.01), and more advanced clinical stage (P&lt;0.01) compared with low lncRNA DANCR expression in cancer populations. High lncRNA DANCR expression was associated with worse prognosis compared with low lncRNA DANCR expression in cancers. LncRNA DANCR expression could serve as a prognostic factor of human cancers.
Up-regulation of miRNA-148a inhibits proliferation, invasion, and migration while promoting apoptosis of cervical cancer cells by down-regulating RRS1
Authors: Zhang Ying , Sun Bingmei , Zhao Lianbin , Liu Zhengling , Xu Zonglan , Tian Yonghui , Hao Changhong 
Published: March 2019

AbstractThe purpose of the present study is to figure out the role of miRNA-148a (miR-148a) in growth, apoptosis, invasion, and migration of cervical cancer cells by binding to regulator of ribosome synthesis 1 (RRS1). Cervical cancer and adjacent normal tissues, as well as cervical cancer cell line Caski, HeLa, C-33A, and normal cervical epithelial cell line H8 were obtained to detect the expression of miR-148a and RRS1. Relationship between miR-148a and RRS1 expression with clinicopathological characteristics was assessed. The selected Caski and HeLa cells were then transfected with miR-148a mimics, miR-148a inhibitors or RRS1 siRNA to investigate the role of miR-148a and RRS1 on proliferation, apoptosis, colony formation, invasion, and migration abilities of cervical cancer cells. Bioinformatics information and dual luciferase reporter gene assay was for used to detect the targetting relationship between miR-148a and RRS1. Down-regulated miR-148a and up-regulated RRS1 were found in cervical cancer tissues and cells. Down-regulated miR-148a and up-regulated RRS1 are closely related with prognostic factors of cervical cancer. RRS1 was determined as a target gene of miR-148a and miR-148a inhibited RRS1 expression in cervical cancer cells. Up-regulation of miR-148a inhibited cell proliferation, migration, and invasion while promoting apoptosis in Caski and HeLa cells. Our study suggests that miR-148a down-regulates RRS1 expression, thereby inhibiting the proliferation, migration, and invasion while promoting cell apoptosis of cervical cancer cells.
Polymorphisms inCLDN1are associated with age and differentiation of triple-negative breast cancer patients
Authors: Hu Aimin , Li Junyu , Ruan Shufang , Fan Ying , Liao Yuqian 
Published: March 2019

AbstractPurpose: Triple-negative breast cancer (TNBC) is a highly heterogeneous disease. It is very important to explore novel biomarkers to better clarify the characteristics of TNBC. It has been reported that polymorphisms in claudin 1 (CLDN1) are associated with risk of several cancers. But till now, there is no report about these polymorphisms and TNBC. Patients and methods: Between January 2004 and December 2013, 267 patients with stage I–III primary TNBC were included in our study. We investigated the association between polymorphisms in CLDN1 gene and clinicopathological characteristics or survival of these patients. We used Haploview 4.2 software to identify Tag single nucleotide polymorphisms (SNPs). MassARRAY MALDI-TOF System was used for genotyping. Results: We found that rs10513846 GA genotype was associated with older age [P=0.013, hazard ratios (HR) = 2.231, 95% confidence interval (CI): 1.186–4.195]. Rs10513846 AA genotype carriers were more likely to develop grade 3 tumors (P=0.005, HR = 2.889, 95% CI: 1.389–6.007). And rs9283658 genotypes were also related to grade, more patients with grade 3 tumors were rs9283658 CC genotype carriers (P=0.023, HR = 0.446, 95% CI: 0.222–0.894). There was no association between polymorphisms in CLDN1 and survival of TNBC patients. After multivariate analysis, tumor size (P=0.021, HR = 3.146, 95% CI: 1.185–8.354) and lymph node status (P&lt;0.001, HR = 10.930, 95% CI: 3.276–36.470) were demonstrated to be independent prognostic factors. Conclusion: We first demonstrated that polymorphisms in CLDN1 gene were associated with age and differentiation of TNBC patients.
Inhibition of acid sphingomyelinase activity ameliorates endothelial dysfunction in db/db mice
Authors: Jiang Meng , Huang Shanya , Duan Wang , Liu Qiaoshu , Lei Minxiang 
Published: March 2019

Abstract
Acid sphingomyelinase (aSMase) plays an important role in endothelial dysfunction. Here, we show that elevated aSMase activity and ceramide content were reduced by desipramine treatment in diabetic animals. The inhibitor of aSMase, desipramine, improved vascular dysfunction in db/db mice. High glucose (HG)-induced up-regulation of aSMase activity and ceramide levels were restored by treatment with aSMase siRNA or desipramine in endothelial cells. In addition, aSMase siRNA or desipramine treatment increased the release of nitric oxide (NO) and the phosphorylation of endothelial NO synthase (eNOS) in diabetic mouse aortas and aortic endothelial cells with HG.
These results indicate that inhibition of aSMase/ceramide pathway improves endothelium-dependent vascular relaxation (EDR) largely through regulating the eNOS/NO pathway in diabetic animals.
NFKB1 gene rs28362491 polymorphism is associated with the susceptibility of acute coronary syndrome
Authors: Jin Si-Yu , Luo Jun-Yi , Li Xiao-Mei , Liu Fen , Ma Yi-Tong , Gao Xiao-Ming , Yang Yi-Ning 
Published: March 2019

Abstract
The acute coronary syndrome (ACS) is a complex disease where genetic and environmental factors are involved. NF-κB, a central regulator of inflammation, is involved in various inflammatory diseases. The aim of the present study was to explore the association between NFKB1 gene rs28362491 (-94ATTGins/del) polymorphism and ACS. A total of 778 ACS patients and 1112 healthy subjects were included in our study. The TaqMan SNP genotyping assays was used to analyze the rs28362491 polymorphism. The lesion extent of coronary artery was assessed by Gensini Score and lesion vessel number in ACS patients. For total and males, the frequencies of the mutant DD genotype and D allele were significantly higher in ACS patients than that in control subjects (total: DD genotype: 18.0 vs 14.1%, P=0.009, D allele: 43.0 vs 37.9%, P=0.002, males: DD genotype: 20.6 vs 15.3%, P=0.042, D allele: 44.2 vs 38.8%, P=0.013). After multivariate logistic regression analysis, we found that individuals with mutant DD genotype had 1.329-fold higher risk of ACS compared with individuals with ID and II genotypes. Moreover, ACS patients with DD genotype were worse stenosis of coronary artery compared with patients carrying II or ID genotype. In conclusion, our study demonstrated that the mutant DD genotype of NFKB1 gene was associated with the risk and severity of ACS in Han population in Xinjiang, northwest of China.
Extensive mechanical tension promotes annulus fibrosus cell senescence through suppressing cellular autophagy
Authors: Zhao Liang , Tian Baofang , Xu Qing , Zhang Cunxin , Zhang Luo , Fang Haolin 
Published: March 2019

AbstractBackground: Mechanical load contributes a lot to the initiation and progression of disc degeneration. Annulus fibrosus (AF) cell biology under mechanical tension remains largely unclear.Objective: The present study was aimed to investigate AF cell senescence under mechanical tension and the potential role of autophagy.Methods: Rat AF cells were cultured and experienced different magnitudes (5% elongation and 20% elongation) of mechanical tension for 12 days. Control AF cells were kept static. Cell proliferation, telomerase activity, cell cycle fraction, and expression of senescence-related molecules (p16 and p53) and matrix macromolecules (aggrecan and collagen I) were analyzed to evaluate cell senescence. In addition, expression of Beclin-1 and LC3, and the ratio of LC3-II to LC3-I were analyzed to investigate cell autophagy.Results: Compared with the control group and 5% tension group, 20% tension group significantly decreased cell proliferation potency and telomerase activity, increased G1/G0 phase fraction, and up-regulated gene/protein expression of p16 and p53, whereas down-regulated gene/protein expression of aggrecan and collagen I. In addition, autophagy-related parameters such as gene/protein expression of Beclin-1 and LC3, and the ratio of LC3-II to LC3-I, were obviously suppressed in the 20% tension group.Conclusion: High mechanical tension promotes AF cell senescence though suppressing cellular autophagy. The present study will help us to better understand AF cell biology under mechanical tension and mechanical load-related disc degeneration.
Association between CYP3A4 gene rs4646437 polymorphism and the risk of hypertension in Chinese population: a case–control study
Authors: Wang Juan , Ji Hongliang , Jia Helei , Guan Dongsheng 
Published: March 2019

Abstract
Using a case–control design, we assessed the association between single nucleotide polymorphisms of CYP3A4 gene rs4646437 polymorphism and the risk of hypertension in Chinese population. We recruited 450 hypertension patients from The First Clinical College, Henan University of Chinese Medicine between June 2017 and May 2018. There was a significant difference in genotype distribution between case group and control group (χ2=18.169, P=0.000). The minor A allele was significantly higher in the case group than that in the control group (31.0 vs 24.8%, P=0.000, odds ratio [OR]=1.36, 95% confidence interval [95% CI]: 1.12–1.66). Significant differences were also observed in other gene models: the GA/AA genotype did not increase the risk of hypertension compared with GG genotype (OR=1.16, 95% CI: 0.90–1.49, P=0.259). Compared with GG/GA genotype, the AA genotype also increased the risk of hypertension (OR=2.34, 95% CI: 1.56–3.50, P=0.000). For additive model, the AA genotype was significantly associated with GG genotype (OR=2.25, 95% CI: 1.49–3.42, P=0.000). The same results were found for AA vs GA (OR=2.50, 95% CI: 1.60–3.89, P=0.000). For the allele genotype, the A allele frequency was significantly higher in the case group than that in the control group (31.0 vs 24.8%, P=0.002). The A allele of CYP3A4 rs4646437 was associated with an increased risk for hypertension (OR=1.36, 95% CI: 1.12–1.66, P=0.002). Our results revealed a possible genetic association between CYP3A4 gene rs4646437 and hypertension, and the AA genotype of rs4646437 increased the risk of hypertension in Chinese Han population, and this effect could be confirmed by multivariable analyses.
In-situ real-time monitoring of spurious modes in HE11 transmission lines using multi-hole couplers in miter bends
Authors: Zach Alexander , Kasparek Walter , Lechte Carsten , Plaum Burkhard , Monaco Francesco , Schütz Harald , Stober Jörg , Idei Hiroshi , Hirth Thomas 
Published: March 2019

Transmission of high-power millimeter waves for ECRH is often realised with oversized corrugated circular waveguides. Coupling from the gyrotron source to the waveguide is typically done via matching mirrors in free space. Small alignment errors of the system lead to the excitation of higher-order modes inside the waveguide beside the main transmission mode HE11. Those modes have comparably higher losses and can in worst case result in local fields exceeding the breakdown limit of the medium inside the waveguide.
For alignment control over the whole pulse duration of the gyrotron, a set of hole-array couplers placed into a miter bend mirror probes the field inside the waveguide. The arrays are designed to detect the marker modes for beam oﬀset and tilt (LP(e=o)11 )as well as for beam waist mismatch (LP02). In addition, a main mode coupler sensitive mostly for the HE11 content is used as a power monitor. By maximizing the signal of the power monitor and minimizing the content of marker modes, a first-order optimization of the coupling from free space to the waveguide can be achieved. Signal processing of the 140 GHz information is done at kHz range after downmixing, using a frequency shifted part of the power monitor signal.
As the measurement system is placed in a miter bend mirror, it can also be easily installed at various locations along the transmission line to check for possible misalignments of the waveguide connections between miter bends. Simulation and low power experimental results will be shown.
Schwarzkümmel: Medizin-Tipp aus der Bibel
Authors: Vlachojannis Julia , Chrubasik-Hausmann Sigrun 
Published: March 2019

ZusammenfassungSeit dem Altertum wird der Schwarzkümmel, auch Gold der Pharaonen genannt, als Heilmittel geschätzt. Der Wirkstoff besitzt vorbeugende und heilende Wirkungen. Verschiedene experimentelle Untersuchungen untermauern die antientzündliche Wirkung. Der Antikrebswirkung liegt eine antiproliferative, proapoptotische, antioxidative, zytotoxische und antimetastatische Wirkung zugrunde. Außerdem stimuliert der Schwarzkümmel-Wirkstoff die Killerzellen im Blut. Wie synthetische Zytostatika wirkt die Leitsubstanz Thymochinon alkylierend. Tierexperimentell besaß der Schwarzkümmel in verschiedenen Modellen eine organprotektive Wirkung und reduzierte den durch Galaktose ausgelösten Alterungsprozess. Schwarzkümmelzubereitungen wirkten u. a. hypoglykämisch, blutdrucksenkend, antiasthmatisch, spasmolytisch, antidepressiv, antimikrobiell, antiviral und antiparasitär. Das präklinische Sicherheitsprofil ist inkomplett. Nach Zufuhr größerer Mengen ist mit toxischen Wirkungen zu rechnen.
Professionelle Pflege von suizidalen
Menschen
Authors: Höhne Lance 
Published: March 2019

ZusammenfassungWie können die beziehungsgestaltenden und interaktiven Elemente der
professionellen Pflege bei Suizidalität nach Bernd Kozel unter Berücksichtigung
des Primary-Nursing-Konzepts nach Marie Manthey umgesetzt werden? Dieses
umfangreiche und spannende Thema stellen wir Ihnen in dieser und der nächsten
Ausgabe vor. Im ersten Teil beleuchten wir das Konzept von Kozel und seine
Kernelemente.
Comparison of Game Movement Positional Profiles Between Professional Club and Senior International Rugby Union Players
Authors: Beard Adam , Chambers Ryan , Millet Gregoire P. , Brocherie Franck 
Published: March 2019

AbstractThe purpose of this study was to compare the game movement demands between professional club and senior international rugby union players. Data were obtained from 188 players from 4 professional club teams (Rabo Direct Pro12) and the affiliated international team during the 2014–15 season. Players were tracked by global positioning system (GPS) sampled at 10 Hz and were categorized into 6 different positional groups (front row forwards, FRF; second row forwards, SRF; back row forwards, BRF; half backs, HB; centres, C; outside backs, OB) and separated into playing standard (club vs. international level). Data on distance, distance per minute, high speed running, maximum velocity, sprint distance and efforts as well as repeated high-intensity locomotion efforts (RHILE) were collected. Significant effects (P<0 .05) between club and international were found for RHILE in all 6 positional groupings with a higher number of RHILE in international vs. club games. Significantly (P<0.05) greater total distance and meterage were also shown in international compared to club for OB position. The RHILE differences between club and international games whatever the positions appear of practical relevance for coaches and performance staff to concentrate on training protocols to enhance this quality as well as evaluation methods.
Stereoselective Aldol Additions of Glycolic Acid and Its Derivatives
Authors: Engesser Tobias , Brückner Reinhard 
Published: March 2019

This review gives a comprehensive overview of aldol additions of glycolic acid derivatives to achiral aldehydes and acetals affording α,β-dihydroxycarboxylic acids or derivatives thereof. The focus is on simple diastereoselectivity. A selection of related aldol additions is also presented: aldol additions of glycolic acid derivatives to ketones with two different substituents and aldol additions of α-substituted glycolic acid derivatives.1	Introduction1.1	Organization of this Review1.2	Outside the Scope of this Review: Aldol Additions Giving α,β-Dihydroxyaldehydes and α,β-Dihydroxyketones Diastereoselectively1.3	Non-Aldol Routes to Diastereomerically Pure α,β-Dihydroxycarboxylic Acid Derivatives2	Aldol Additions of Glycolic Acid (Derivative) Enolates to Aldehydes2.1	Aldol Additions of Glycolate Enolates (‘Glycolic Acid Dianions’)2.2	Aldol Additions of Glycolic Ester Enolates2.3	Aldol Additions of Glycolic Thioester Enolates2.4	Aldol Additions of Glycolimide Enolates2.5	Aldol Additions of Glycolamide Enolates2.6	Mukaiyama Aldol Additions of Silyl Ketene Acetals of Glycolic Esters­ and Thioesters2.7	Aldol Additions of Glycoloyl Chlorides via Acyl Ammonium Enolates3	Aldol-Providing Substitutions of Glycolimide Enolates in Acetals4	Additions Related to Aldol Additions of Glycolic Acid Derivative Enolates to Aldehydes4.1	Selected Simply Diastereoselective Aldol Additions of Enolates of Glycolic Acid Derivatives to Ketones with Two Different Substituents4.2	Simply Diastereoselective Aldol Additions of Enolates of Glycolic Acid Derivatives with a Methyl Substituent at C-α (Lactic Acid Derivatives)5	Conclusion
Recent Advances in Mainstream Hemostasis Diagnostics and Coagulation Testing
Authors: Favaloro Emmanuel , Lippi Giuseppe 
Published: March 2019

AbstractThe authors provide a narrative review of mainstream hemostasis analyzers. The review is presented in two parts. In the first part, the authors give voice to several internationally representative manufacturers of mainstream hemostasis analyzers. They ask these manufacturers to (1) answer a series of survey questions that may otherwise reflect common questions asked by users or potential users of their equipment, (2) provide a bullet list of their main focus areas, and (3) provide other information in regard to their instrumentation. The authors then offer their own personal perspective of the benefits of various aspects of such equipment, as well as a sampling of some hemostasis tests as currently available, or potentially in progress. It is hoped that this snapshot of mainstream hemostasis diagnostics and coagulation testing drives positive future changes that will ultimately provide the best outcomes for laboratories performing hemostasis assays and the patients they help diagnose and manage.
Resultados da orientação à prática de atividade física em crianças e adolescentes obesos
Authors: Valadão Rita , Miraglia Fernanda , Beghetto Mariur , Mello Elza 
Published: March 2019

Resumo
Introdução Diferentes métodos para tratar crianças e adolescentes com excesso de peso têm sido amplamente estudados, entre esses, a prática de atividade física. Um programa de atividade física que possa ser realizado no domicílio e aplicado no ambiente Sistema Único de Saúde (SUS), com esse intuito, ainda é inédito.
Objetivo Avaliar o nível de atividade física (AF) de crianças e adolescentes obesas, após orientação para executarem AF em casa e sua associação com escore-Z IMC (índice de massa corporal), escore-Z peso, percentuais de Massa Livre de Gordura (MLG), Massa Gorda (MG) e Taxa Metabólica Basal (TMB).
Método Foram acompanhados por um ano 15 meninas e 12 meninos com médias de idade de 11,29 ± 1,92 anos e percentil IMC P >98. O teste de impedância bioelétrica (BE) foi uitilizado para mensurar os percentuais de MLG, e MG e a TMB. O questionário International Physical Activity Questionnaire (IPAQ) foi utilizado para mensurar o nível de AF.
Resultados Verificou-se que crianças e adolescentes conseguem seguir uma rotina de AF em ambiente doméstico a partir de orientações mensais. Houve melhora de escore-z de peso em 59,3% dos pacientes, e do escore-z do IMC, em 77,8%, a TMB e a MLG aumentou em 96,3% da amostra, a MG diminuiu em 33,3% da amostra, durante o período de intervenção de 12 meses. Não se obteve êxito em diminuir as horas de sedentarismo para menos de duas por dia na maior parte da amostra.
Conclusão Crianças e adolescentes, se estimuladas, conseguem melhorar seus níveis de AF, passando de inativos para ativos, verificou-se ainda, a modificação de sua composição corporal neste período de 1 ano. A AF, mesmo que de forma isolada, colabora positivamente no manejo do excesso infanto-juvenil.
Sensibilidade não-celíaca ao glúten
Authors: Boarim Daniel 
Published: March 2019

ResumoEntre as reações adversas a alimentos, os distúrbios relacionados ao glúten vêm atualmente ganhando cada vez mais espaço nas publicações científicas e não científicas. Estes distúrbios são divididos em três entidades: doença celíaca (DC), alergia ao trigo (AT) e sensibilidade não-celíaca ao glúten ou ao trigo (SNCG/T), considerando que este cereal vem sendo associado mais frequentemente às manifestações, discutindo-se ainda se não seria o real componente alimentar desencadeante. A DC é uma enteropatia autoimune, enquanto a AT é uma reação de hipersensibilidade, mediada ou não mediada pela imunoglobulina E (IgE). A SNCG/T é uma entidade emergente, de fisiopatologia ainda discutida, que vários pesquisadores propõem como diagnóstico de exclusão em relação à AT e à DC. A SNCG/T vem despertando grande celeuma popular e polêmica nos meios médicos. A presente revisão tem por objetivo abordar a SNCG/T, procurando elencar elementos de distinção dos outros dois distúrbios retrocitados discutindo aspectos como características clínicas, diagnóstico diferencial, fisiopatologia e tratamento. Em muitos trabalhos, salienta-se a possibilidade de sobreposição ou de confusão diagnóstica com outras doenças funcionais gastrointestinais, como síndrome do intestino irritável (SII) e oligossacarídeos, dissacarídeos, monossacarídeos e polióis fermentáveis (FODMAPs, na sigla em inglês). Concluímos que a SNGG/T, embora constitua uma entidade clínica reconhecida por um número expressivo de pesquisadores, ainda é objeto de amplo questionamento quanto à sua caracterização, ao seu diagnóstico e à sua fisiopatologia. Isto posto, considerando sua elevada prevalência estimada e as repercussões populares, indubitavelmente trata-se de assunto digno de estudos mais aprofundados e bem conduzidos.