bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification ofGanodermaDisease Resistance Loci Using Natural Field Infection of an Oil Palm Multiparental Population
Authors: Tisné Sébastien , Pomiès Virginie , Riou Virginie , Syahputra Indra , Cochard Benoît , Denis Marie 
Published: January 2019

AbstractMulti-parental populations are promising tools for identifying quantitative disease resistance loci. Stem rot caused by Ganoderma boninense is a major threat to palm oil production, with yield losses of up to 80% prompting premature replantation of palms. There is evidence of genetic resistance sources, but the genetic architecture of Ganoderma resistance has not yet been investigated. This study aimed to identify Ganoderma resistance loci using an oil palm multi-parental population derived from nine major founders of ongoing breeding programs. A total of 1200 palm trees of the multi-parental population was planted in plots naturally infected by Ganoderma, and their health status was assessed biannually over 25 yr. The data were treated as survival data, and modeled using the Cox regression model, including a spatial effect to take the spatial component in the spread of Ganoderma into account. Based on the genotypes of 757 palm trees out of the 1200 planted, and on pedigree information, resistance loci were identified using a random effect with identity-by-descent kinship matrices as covariance matrices in the Cox model. Four Ganoderma resistance loci were identified, two controlling the occurrence of the first Ganoderma symptoms, and two the death of palm trees, while favorable haplotypes were identified among a major gene pool for ongoing breeding programs. This study implemented an efficient and flexible QTL mapping approach, and generated unique valuable information for the selection of oil palm varieties resistant to Ganoderma disease.
Using Next-Generation Sequencing Transcriptomics To Determine Markers of Post-traumatic Symptoms: Preliminary Findings from a Post-deployment Cohort of Soldiers
Authors: Boscarino Cathy , Nalpathamkalam Thomas , Pellecchia Giovanna , Li Weili , Thiruvahindrapuram Bhooma , Merico Daniele 
Published: January 2019

Abstract
Post-traumatic stress disorder is a concerning psychobehavioral disorder thought to emerge from the complex interaction between genetic and environmental factors. For soldiers exposed to combat, the risk of developing this disorder is twofold and diagnosis is often late, when much sequela has set in. To be able to identify and diagnose in advance those at “risk” of developing post-traumatic stress disorder, would greatly taper the gap between late sequelae and treatment. Therefore, this study sought to determine whether the transcriptome can be used to track the development of post-traumatic stress disorder in this unique and susceptible cohort of individuals. Gene expression levels in peripheral blood samples from 85 Canadian infantry soldiers (n = 58 participants negative for symptoms of post-traumatic stress disorder and n = 27 participants with symptoms of post-traumatic stress disorder) following return from deployment to Afghanistan were determined using RNA sequencing technology. Count-based gene expression quantification, normalization and differential analysis (with thorough correction for confounders) revealed genes associated to PTSD; LRP8 and GOLM1. These preliminary results provide a proof-of-principle for the diagnostic utility of blood-based gene expression profiles for tracking symptoms of post-traumatic stress disorder in soldiers returning from tour. It is also the first to report transcriptome-wide expression profiles alongside a post-traumatic symptom checklist.
Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications
Authors: Dunst Sebastian , Tomancak Pavel 
Published: January 2019

Abstract
The development of fluorescent labels and powerful imaging technologies in the last two decades has revolutionized the field of fluorescence microscopy, which is now widely used in diverse scientific fields from biology to biomedical and materials science. Fluorescence microscopy has also become a standard technique in research laboratories working on Drosophila melanogaster as a model organism. Here, we review the principles of fluorescence microscopy technologies from wide-field to Super-resolution microscopy and its application in the Drosophila research field.
Vygotski’s theories on Defectology: contributions to the special education of the 21st century
Authors: Barroco Sônia Mari Shima 
Published: January 2019

This text exposes the pertinence of L. S. Vygotski’s (1896-1934) theoretical-methodological assertions to the Soviet Defectology at its original birth, when our today’s Basic Education universalization did not exist yet, to Special Education of the 21st century, when the right to Education in democratic states is regarded as a means of social and educational inclusion. The author turns out to be revolutionary for subsidizing an integrated vision of the constitution of the human psychic activity, defending the plain possibility of humanization, the formation of cultural building of human species in both ableb and disabled people. *** Teorizações de Vygotski sobre a Defectologia: contribuições à educação especial do século XXI ***O texto expõe a pertinência das asserções teórico-metodológicas de L. S. Vygotski (1896-1934) à Defectologia Soviética em seu contexto original, quando a Educação Básica não era universalizada, e as coloca em perspectiva para o século XXI, quando a Educação é assumida como direito para todos, nos Estados democráticos e sob a perspectiva da inclusão social e educacional. O autor revela-se revolucionário por subsidiar uma visão integrada da constituição do psiquismo humano, por defender a possibilidade de humanização, a formação do edifício cultural nas pessoas com e sem deficiências.Palavras-chave: Vygotski; Educação Especial; Defectologia.
USAHA BUDIDAYA IKAN LELE (Clarias Sp) PADA KAWASAN MINAPOLITAN “KAMPUNG LELE” KABUPATEN BOYOLALI
Authors: Firdaus Maulana , Putri Hertria , Hafsaridewi Rani 
Published: January 2019

AbstrakKabupaten Boyolali merupakan salah satu kawasan minapolitan yang ada di Jawa Tengah, yang ditetapkan melalui Keputusan Menteri Kelautan dan Perikanan RI Nomor KEP.32/MEN/2010 tentang Penetapan Kawasan Minapolitan. Berdasarkan keputusan tersebut, pengembangan usaha perikanan khususnya budidaya ikan lele (Clarias Sp) semakin gencar dilakukan. Tujuan dari penelitian ini untuk mengetahui gambaran pola pengelolaan, permasalahan yang dihadapi, pemasaran hasil produksi, serta memberikan gambaran terhadap struktur biaya, penerimaan, keuntungan dan nilai R/C ratio. Metode penelitian yang digunakan adalah studi kasus, dengan pemilihan sampel menggunakan purposive sampling. Berdasarkan hasil analisis, usaha budidaya ikan lele di Desa Tegal Rejo secara keseluruhan masih layak diusahakan karena memiliki nilai R/C ratio > 1. Permasalah usaha yang dihadapi oleh pembudidaya terkait dengan ketersediaan benih, kenaikan harga pakan, harga jual ikan lele dan serangan penyakit. Dalam menjaga keberlanjutan usaha budidaya ikan lele pada kawasan minapolitan ”Kampung Lele” Desa Tegal Rejo, perlu diperhatikan daya dukung lingkungan serta pengelolaan usaha yang baik.Title: CATFISH AQUACULTURE BUSSINESS AT MINAPOLITAN AREA “KAMPUNG LELE” IN THE BOYOLALI DISTRICT Abstract Boyolali is the one of Minapolitan Area in Central Java, which are determined by the Minister of Marine and Fisheries Affairs, through by the minister decision KEP.32/MEN/2010. Based on these decision, the development of the fisheries, especially catfish (Clarias SP) aquaculture more improved conducted. This study aimed to know the description of management patterns, problems faced, the marketing of production, as well as give an illustrate of the cost structure, revenue, profits and the value of R/C ratio. Case study method were used in this research, sample selected by purposive sampling method. Based on the analysis, the aquaculture bussiness of catfish in Tegal Rejo as a whole is still viable because it has a value of R/C ratio > 1. Business problems faced by farmers related to the availability of seed, feed price increases, prices of catfish and disease attack. In maintaining the sustainability of catfish farming in the region Minapolitan "Kampung Catfish" Tegal Rejo village, keep in mind the carrying capacity of the environment and good business management.
Correction of High Corneal Astigmatism during Cataract Surgery
Authors: Pershin K. B. , Pashinova N. F. , Konovalov M. E. , Gurmizov E. P. , Zubenko O. Yu. , Tsygankov A. Yu. 
Published: January 2019

Patients, who need cataract surgical treatment, often fail to achieve a high uncorrected visual acuity after surgery due to the concomitant astigmatism involved. Currently, surgeons are increasingly performing combined surgical interventions, including relaxing limbal keratotomic incisions (manual keratotomy) or femtoarcuatous keratotomy, as well as implanting toric intraocular lenses. In recent years, additional toric intraocular lenses have been available. Purpose: to analyze our own clinical experience of implanting an additional toric intraocular lens to correct corneal astigmatism in three clinical cases. The article presents our clinical experience of successful correction of residual corneal astigmatism after previous cataract phacoemulsification with the implantation of a monofocal toric intraocular lens in three patients patients aged 70, 61 and 54 years. In all cases, an additional toric intraocular lens Add-on Torica-sPB pre-filled in the cartridge with a good refractive effect was implanted. The uncorrected visual acuity was 1.0 in all the investigated cases at the follow-upo period of 6 months after the surgical intervention. Calculation of the toric intraocular lens optical power was performed using an online calculator. A feature of surgical intervention was the repositioning of the additional toric intraocular lens into the ciliary sulcus. Changes in the data of keratotopography before and after surgery were absent. In none of the investigated cases, intra- and postoperative complications and dislocation of the implanted additional toric intraocular lens were determined. Based on these cases, high predictability, efficacy and safety of implantation of an additional toric intraocular lens are shown, in the case of residual middle-grade corneal astigmatism after the initial cataract phacoemulsification with the toric intraocular lens implantation. This approach can be successfully used in patients during one-stage surgical treatment of cataract and associated high-grade corneal astigmatism, expanding existing protocols for the treatment of this group of patients.
Homeostasis Changes of Tear-Producing System with the Background of Cosmetic Procedures in Periorbital Area
Authors: Trubilin V. N. , Poluninа E. G. , Andzhelova D. V. , Kurenkov V. V. , Kapkova S. G. , Chinenova K. V. 
Published: January 2019

Modern ophthalmological practice is inseverable linked with cosmetology and esthetic medicine. Every year, the number of patients, especially women, who received various types of cosmetology procedures (botulinum toxin injections, eyelash extensions, eyelid tattooing, dermal fillers injections, etc.) in facial area increases, in particular in the periorbital zone.Purpose: to conduct a comprehensive assessment of clinical and functional parameters characterizing the state of the ocular surface and the tear-producing system in case applying cosmetic procedures in the periorbital area (Botox injections, eyelid tattooing, eyelash extension).Patients and methods. The study included 106 women (212 eyes). Exclusion criteria: early postoperative period after ophthalmosurgical intervention, acute inflammatory diseases of anterior and posterior eye segment, glaucoma, the presence of intraocular pathological changes (hemophthalmia, detachment of the eye membranes, tumors). The average age of the patients included in the study was 36.37 ± 9.0 years.Results and discussion. The results of a comparative evaluation of two groups of patients — a group with a history of cosmetic procedures (tattooing the eyelids, botulinum toxin, dermal fillers, eyelash extensions) in the periorbital zone and patients who did not use such procedures, has showed that cosmetic procedures affect on the clinical and functional eye surfaces state and on morphofunctional state of meibomian glands. Thus, the frequency of detection of symptomocomplex of dry eye syndrome was on 36 % more often determined in the group of patients received cosmetic procedures. It has been shown that the combined effect of cosmetological procedures (tattooing the eyelids, eyelash extensions, injections of dermal fillers and botulinum toxin in the periorbital region) aggravates the course and prognosis of Dry Eye Syndrome and meibomian glands dysfunction. A direct correlation between the severity of complaints characteristic of Dry Eye Syndrome and the number of procedures was found (r = 0.4982, p = 0.0000); the frequency of detecting complaints of dryness and discomfort and the number of procedures (r = 0.6427, p = 0.0000); compression test indices and the number of procedures (r = –0.4712, p = 0.0000); the frequency of occurrence of inflammatory reactions of ocular surface and the number of procedures (r = 0.3778, p = 0.0001). The risk of tear production disturbance — dry eye syndrome and dysfunction of the meibomian glands is higher in patients using botulinum toxin injections and eyelash extension in comparison with patients using eyelid tattooing and injections of fillers with hyaluronic acid in the periorbital zone.
Multi-Factoral Mechanisms of Therapeutic Effect of Perifocal Spectacles (Perifocal-M) on Progressive Myopia in Children
Authors: Ibatulin R. A. , Proskurina O. V. , Tarutta E. P. 
Published: January 2019

There is detailed review of multi-factoral mechanisms of spectacles with peripheral defocus influence in children with progressive myopia in this article. At present local retinal mechanisms of neuro-regulatory control of eye growth are most effective in prevention of myopia development and progression. Optical correction of relative peripheral hyperopia with following peripheral myopic defocus affects the biochemical cascade from retina to choroid and sclera, which constrains an eye growth. Optical methods of myopia control, including spectacles, widely used in native and foreign ophthalmology practice. Since 2011, in Russia Perifocal-M spectacles are using, said spectacles provides bigger functionality of influence on myopic eye comparing to foreign analogues. Construction features of said spectacle lens have a complex influence on different optic-physiological eye structures and each of said structures affects refractogenesis. Perifocal spectacles takes into account specific for myopic eye features of central and peripheral refraction along horizontal and vertical meridians. They have stronger refraction in horizontal, which allows to eliminate specific optical disbalance in myopic eye and to create optical balance in the eye. Perifocal-M lens construction with asymmetric horizontal progression allows to correct relative peripheral hyperopia, to create myopic defocus in horizontal and to affect relations between refraction of nasal and temporal halves of retina. The latter is due to the fact that optical influence starts earlier and has bigger effect on nasal half of retina relative to temporal. Perifocal spectacles are inducing positive spherical aberrations in the eye, increasing accommodation functions, supporting high binocular visual acuity, improving binocular interaction during near work, fighting against heteroforias. Thus, optical features of Perifocal-M spectacles for the first time creates conditions for versatile functional influence on main myopogenic factors simultaneously, it effectively stops myopia progression.
Сolor Doppler Imaging in Eye Hemodynamics Evaluation in Syndrome of Thrombotic Microangiopathy
Authors: Smirnova T. V. , Kozlovskaya N. I. , Sheludchenko V. M. , Andzhelova D. V. , Kazaryan E. E. , Eksarenko O. V. , Velieva I. A. , Khasyanova M. V. 
Published: January 2019

Purpose.To assess the state of blood flow in retrobulbar vessels using the method of color Doppler imaging in thrombotic microangiopathy (TMA) associated with atypical hemolytic uremic syndrome (aHUS), malignant hypertension (MH) and catastrophic antiphospholipid syndrome (CAPS).Methods. The study involved 16 patients aged from 18 to 43 years with TMA associated with aHUS (13 patients), MH (2 patients) and CAPS (1 patient). All patients underwent a study of the state of blood flow in the vessels of the retrobulbar space by color Doppler imaging using a multifunctional ultrasonic diagnostic device Voluson 730 Pro and Voluson E8. The spectrum of blood flow in the ophthalmic artery (OA), central retinal artery (CRA), central retinal vein (CRV), in the medial and lateral short posterior ciliary arteries (SPCA) was recorded and the following parameters were determined: maximum systolic velocity (Vsyst), final diastolic velocity (Vdiast), peripheral resistance index (RI), pulsation index (PI).Results. The analysis of the Doppler blood flow spectrum in all patients with TMA syndrome revealed a decrease in Vsyst in the vessels of the retrobulbar space, more pronounced in the CRA, with an increase in the venous component of the Doppler spectrum. In patients with TMA associated with aHUS and MH Vdiast in the studied vessels were in normal limits or increased, RI and PI were reduced. In aHUS a statistically significant correlation was found between Vdiast in OA and the level of haptoglobin in blood serum, which is a classic marker of intravascular hemolysis.Conclusion. Color Doppler imaging of retrobulbar vessels is an important method for the diagnosis of ocular hemodynamics disorders in the syndrome of TMA. All patients with TMA syndrome are characterized with a decrease in the systolic component of the Doppler spectrum of blood flow in the vessels of the retrobulbar space, especially in the CRA, with an increase in the venous component. With aHUS and MH diastolic blood flow in the retrobulbar vessels corresponds to the norm or increases, RI and PI decrease. In TMA associated with aHUS, the final diastolic velocity of blood flow in OA depends on the level of microvascular hemolysis.
Еpidemiological, Etiological and Clinical Aspects of Acute Infectious Conjunctives — at the Crossroads of Ophthalmology and Epidemiology (Clinical and Laboratory Study)
Authors: Chernakova G. M. , Maychuk D. Yu. , Murtazalieva S. M. , Slonimsky Yu. B. , Kleshcheva E. A. , Yatsyshina S. B. , Ageeva M. R. 
Published: January 2019

The clinical and laboratory picture of acute infectious conjunctivitis has undergone significant changes in the last few years. This circumstance, apparently, is associated with the appearance of a large number of both eye and systemic forms of antibacterial, anti-inflammatory, immunocorrecting drugs and their widespread use, often without establishing an etiological factor of inflammation. The objective of our work was to propose a scheme of rational empirical therapy of acute infectious conjunctivitis, based on the potential of modern ophthalmopharmacology; and to study the etiological spectrum and features of the clinical picture of the above diseases, thereupon correcting the proposed scheme according to the obtained data. Material and methods. A retrospective analysis of over 20,000 outpatient charts of patients with acute inflammation of the conjunctiva and a prospective examination of 275 patients with acute infectious conjunctivitis was carried out. The method of polymerase chain reaction (quantitative and qualitative) was used to identify the genetic material of the pathogen in scrapings from the conjunctiva, the oropharynx and the nasopharynx. Results. The data of the retrospective analysis has shown that when the symptoms indicated acute infectious conjunctivitis, the actual diagnosis of an ophthalmologist was adenoviral conjunctivitis. There were no other nosological forms with the type of pathogen recorded in outpatient charts. The peak incidence was in the winter-spring period, with the highest number of cases in 2011 and 2013 (1509 and 1482 respectively). Results of the prospective study showed the leading role of adenoviruses (20 % of cases), hemophilic rod (18 % of cases) and pneumococcus (12 % of cases) in the development of acute inflammation of the mucous membrane of the eye. As a concomitant infection, the bacteria of the staphylococcus group were found most often. The spectrum of the revealed pathology confirmed the validity of the selected empirical therapy. Conclusion. The results of the study allow us to recommend the prescription of three groups of drugs (antibiotic, antiviral, non-steroidal anti-inflammatory drug) in the treatment of acute infectious conjunctivitis.
Pathology of the Organs of Vision in Metallurgists
Authors: Onischenko A. L. , Kolbasko A. V. , Melnichenko M. A. , Filimonov S. N. 
Published: January 2019

The frequency and structure of ophthalmopathology in workers of the Novokuznetsk Metallurgical Combine (NMC) have been studied. In total 1911 workers of NMC have been examined. The control group comprised 1801 people working in hazardous industrial conditions. The experimental group consisted of employees dealing with management, engineering center, accounting (110 people). Their work was not connected with the impact of unfavorable factors of metallurgical production. In the control group, the hazard classes ranged from 3.2 to 3.4. The difference between the classes of hazard impact consisted in an increase of the intensity of the hazardous factors exposure. In the structure of conjunctival diseases we have diagnosed chronic conjunctivitis (198.3 ‰), chronic blepharoconjunctivitis (109.4 ‰), pinguecula (266.7 ‰) and pterygium (7.2 ‰) in metallurgical workers. Pengucules in the control group of workers was not only more often than in engineers, but also larger, often located in the inner and outer corner of the eye, and was detected in workers under 30–35 years. The workers of the main shops suffered more severely from the function of tear production and the stability of the pre-corneal tear film. The miners suffered from the highest frequency of cataract development — 301 ‰. Thus, a high incidence of inflammatory and dystrophic diseases of the eyelids and conjunctiva, cataracts in metallurgy has been established. An increase in the frequency of ophthalmopathology was noted with an increase in the length of service under harmful conditions of metallurgical production. The authors suggest the term “metallurgy ophthalmopathy”, which includes manifestations of chronic conjunctivitis and / or blepharoconjunctivitis against the background of unstable precorneal film and frequent formation of posterior-subcapsular opacities of the lens.