bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct roles for Peyer’s patch B cells for induction of antigen-specific IgA antibody responses in mice administered oral recombinantSalmonella
Authors: Hashizume-Takizawa Tomomi , Shibata Naoko , Kurashima Yosuke , Kiyono Hiroshi , Kurita-Ochiai Tomoko , Fujihashi Kohtaro 
Published: March 2019

AbstractOur previous study demonstrated an indispensable role of Peyer’s patches (PPs) for the induction of antigen-specific secretory (S)IgA antibody responses after oral immunization with recombinant Salmonella expressing fragment C of tetanus toxin (rSalmonella-Tox C). In this study, we defined the PP lymphoid structures and immune cells required for the induction of mucosal SIgA antibody responses. Adoptive transfer of mononuclear cells (MNCs) from PPs into PP-deficient (PP-null) mice failed to elicit tetanus toxoid (TT)-specific mucosal immunity. However, when the same PP MNCs were transferred into lethally irradiated PP-normal recipient mice, PP MNCs preferentially emigrated to recipient PPs, leading to PP lymphoid structures and TT-specific SIgA antibody responses. Significantly reduced numbers of TT-specific IgA antibody-forming cells were detected in the mesenteric lymph nodes (MLNs) and intestinal lamina propria of mice when surface expression of the sphingosine 1-phosphate receptor on lymphocytes was inhibited by its agonist FTY720. However, FTY720 treatment did not alter dendritic cell migration or Salmonella dissemination into these tissues. When rSalmonella-Tox C-stimulated CD4+ T cells isolated from PPs, MLNs and the spleen were co-cultured with B cells from these tissues, significantly increased levels of TT-specific IgA antibody responses were exclusively induced in cultures containing PP B cells. Furthermore, surface IgA+ PP B cells produced TT-specific IgA antibody responses in vitro. These findings suggest that PP lymphoid structures and surface IgA+ PP B cells are essential elements for the induction of antigen-specific intestinal SIgA antibody responses to oral Salmonella.
Reassessing the dosing of cefoxitin prophylaxis during major abdominal surgery: insights from microdialysis and population pharmacokinetic modelling
Authors: Boisson Matthieu , Torres Bruna Gaelzer Silva , Yani Sabrina , Couet William , Mimoz Olivier , Dahyot-Fizelier Claire , Marchand Sandrine , Grégoire Nicolas 
Published: March 2019

Abstract

Objectives
Cefoxitin is frequently used for surgical antibiotic prophylaxis (SAP). Using microdialysis, we evaluated whether the currently recommended dosing regimen is appropriate to maintain cefoxitin subcutaneous tissue concentrations above the MIC for pathogens involved in abdominal surgical site infection.

Methods
Data from eight patients undergoing major abdominal surgery were analysed using population pharmacokinetic modelling, and Monte Carlo simulations were conducted to determine the PTA for aerobic and anaerobic pathogens. ClinicalTrials.gov: NCT02703857.

Results
Only 2.3% and 47.4% of the simulated patients maintained cefoxitin subcutaneous concentrations above the MIC breakpoint for anaerobic (MIC = 16 mg/L) and aerobic (MIC = 8 mg/L) pathogens, respectively. New simulations with administration of a loading dose followed by a constant infusion of cefoxitin were conducted and demonstrate that, notwithstanding using the same total dose per unit of time, continuous infusion of cefoxitin can cover aerobes in 96.6% of the simulated patients, but remains insufficient for anaerobic bacteria.

Conclusions
The recommended dosing regimen of cefoxitin is insufficient for covering the usual bacteria during abdominal surgery. Administration of a loading dose followed by a constant infusion should be considered for aerobic bacteria and cefoxitin should be avoided as SAP for anaerobic bacteria.
Underserved populations with missing race ethnicity data differ significantly from those with structured race/ethnicity documentation
Authors: Sholle Evan T , Pinheiro Laura C , Adekkanattu Prakash , Davila Marcos A , Johnson Stephen B , Pathak Jyotishman , Sinha Sanjai , Li Cassidie , Lubansky Stasi A , Safford Monika M , Campion Thomas R 
Published: March 2019

AbstractObjectiveWe aimed to address deficiencies in structured electronic health record (EHR) data for race and ethnicity by identifying black and Hispanic patients from unstructured clinical notes and assessing differences between patients with or without structured race/ethnicity data.Materials and MethodsUsing EHR notes for 16 665 patients with encounters at a primary care practice, we developed rule-based natural language processing (NLP) algorithms to classify patients as black/Hispanic. We evaluated performance of the method against an annotated gold standard, compared race and ethnicity between NLP-derived and structured EHR data, and compared characteristics of patients identified as black or Hispanic using only NLP vs patients identified as such only in structured EHR data.ResultsFor the sample of 16 665 patients, NLP identified 948 additional patients as black, a 26%increase, and 665 additional patients as Hispanic, a 20% increase. Compared with the patients identified as black or Hispanic in structured EHR data, patients identified as black or Hispanic via NLP only were older, more likely to be male, less likely to have commercial insurance, and more likely to have higher comorbidity.DiscussionStructured EHR data for race and ethnicity are subject to data quality issues. Supplementing structured EHR race data with NLP-derived race and ethnicity may allow researchers to better assess the demographic makeup of populations and draw more accurate conclusions about intergroup differences in health outcomes.ConclusionsBlack or Hispanic patients who are not documented as such in structured EHR race/ethnicity fields differ significantly from those who are. Relatively simple NLP can help address this limitation.
Antecedents: A Half-Century of Imaging the Breast
Authors: Kopans Daniel B 
Published: March 2019

AbstractThe field of Breast Imaging evolved because a fairly small number of dedicated individuals realized the lifesaving potential of detecting breast cancer earlier. They persevered despite persistent efforts to curtail screening. From the first attempts to produce X-ray images of the breast to magnetic resonance and digital breast tomosynthesis, investigators have worked continuously to develop better ways to detect breast cancer at a time when cure is possible, while working continuously to preserve access for women to screening. Consequently, the death rate from breast cancer has declined by more than 40%. Therapy has improved, but therapy saves lives when breast cancers are treated early.
Hidden Between Craft and Industry: Engineering Patternmakers’ Design Knowledge
Authors: Stein Jesse Adams 
Published: March 2019

Abstract
Craft is currently experiencing an academic and popular revival, as evidenced by increasing interest in ‘makers’ and artisanal practices, both within and beyond design history. Yet, in this moment of craft’s resurgence, some aspects are regularly overlooked. Industrial craft in manufacturing, for instance, is a field ripe for closer analysis. Engineering patternmaking is an industrial craft that remains almost invisible in design history, despite the design-related nature of patternmaking, and its centrality to many industrial manufacturing processes. Drawing on oral histories with Australian patternmakers, this article emphasizes that patternmaking is both a manual and intellectual practice that requires thorough knowledge of drawing, materials, geometry, three-dimensional visuality and manufacturing processes planning. Accordingly, I argue that patternmakers possess and enact a specific type of design knowledge, a form of expertise that has thus far been undervalued in both design and craft histories. Making use of Nigel Cross’ influential theorization of ‘designerly ways of knowing’, this article explores the connections and divergences between design and patternmaking knowledge sets, reminding us that the making of manufactured objects is deeply collaborative across professional and class formations. In doing so, I highlight the significance of industrial craft knowledge in the actualization of design. This example has broader historical implications for how design history frames and values the knowledge, skills and influence of those engaged in industrial production.
Insecticidal Effects of Triterpene Glycosides Extracted From Holothuria atra (Echinodermata: Holothuroidea) Against Spodoptera litura (Lepidoptera: Noctuidae)
Authors: Nobsathian Saksit , Ruttanaphan Torranis , Bullangpoti Vasakorn 
Published: March 2019

Abstract
Spodoptera litura (Fabricius) is economically important in many countries. Many biopesticides have been developed and studied to control this pest species; however, few natural products from marine organisms have been studied for their pesticidal efficiency. This study compared the acute contact toxicity of solvent extracted from the viscera of Holothuria atra and isolated triterpene glycoside compounds on third-instar S. litura. Our results showed that crude methanolic extracts were the most toxic to the larvae (LC50 ~ 10.37 mg/ml) compared with other crude extracts and contained the bioactive compound 3,4-dihydroxybenzoic acid (LC50 ~ 6.01 mg/ml). All crude extracts and isolated triterpene glycoside compounds inhibited carboxylesterase activity. The efficiency and effectiveness of H. atra extractions and triterpene glycoside compounds against S. litura indicate their potential use in integrated pest management programs and the overall value of marine species as natural sources of insecticidal compounds.
No Indication of High Host-Plant Specificity in Afrotropical Geometrid Moths
Authors: Holm Sille , Javoiš Juhan , Molleman Freerk , Davis Robert B , Õunap Erki , Roininen Heikki , Tammaru Toomas 
Published: March 2019

Abstract
Specificity is one of the fundamental concepts in ecology. Host specificity of phytophagous insects has been of particular interest because of its crucial role in diversification and life-history evolution. However, the majority of tropical insects remain insufficiently explored with respect to their host-plant relations. A lack of respective data is also hindering the debate over whether higher levels of host-plant specificity prevail in tropical insects compared to temperate ones. We investigated host-plant specificity of forest geometrid moths (Lepidoptera: Geometridae) in equatorial Africa using host-plant acceptability trials with neonate larvae, with the addition of field observations. We compare our experimental data to the (well-known) host-specificity patterns of closely related temperate (hemiboreal) species. Similarly to the temperate region, there were broadly polyphagous tropical species in several clades of Geometridae utilizing hosts belonging to different plant families. Phylogenetic comparative analysis returned no significant differences in host specificity between the two regions. Our study contributes to the evidence that host-plant specificity of herbivores is not necessarily substantially higher in tropical than temperate regions.
Primeval very low-mass stars and brown dwarfs – VI. Population properties of metal-poor degenerate brown dwarfs
Authors: Zhang (张曾华) Z H , Burgasser A J , Gálvez-Ortiz M C , Lodieu N , Zapatero Osorio M R , Pinfield D J , Allard F 
Published: March 2019

ABSTRACT
We presented 15 new T dwarfs that were selected from UKIRT Infrared Deep Sky Survey, Visible and Infrared Survey Telescope for Astronomy , and Wide-field Infrared Survey Explorer surveys, and confirmed with optical to near-infrared spectra obtained with the Very Large Telescope and the Gran Telescopio Canarias. One of these new T dwarfs is mildly metal-poor with slightly suppressed K-band flux. We presented a new X-shooter spectrum of a known benchmark sdT5.5 subdwarf, HIP 73786B. To better understand observational properties of brown dwarfs, we discussed transition zones (mass ranges) with low-rate hydrogen, lithium, and deuterium burning in brown dwarf population. The hydrogen burning transition zone is also the substellar transition zone that separates very low-mass stars, transitional, and degenerate brown dwarfs. Transitional brown dwarfs have been discussed in previous works of the Primeval series. Degenerate brown dwarfs without hydrogen fusion are the majority of brown dwarfs. Metal-poor degenerate brown dwarfs of the Galactic thick disc and halo have become T5+ subdwarfs. We selected 41 T5+ subdwarfs from the literature by their suppressed K-band flux. We studied the spectral-type–colour correlations, spectral-type–absolute magnitude correlations, colour–colour plots, and HR diagrams of T5+ subdwarfs, in comparison to these of L–T dwarfs and L subdwarfs. We discussed the T5+ subdwarf discovery capability of deep sky surveys in the 2020s.
Cell membrane-covered nanoparticles as biomaterials
Authors: Xuan Mingjun , Shao Jingxin , Li Junbai 
Published: March 2019

AbstractSurface engineering of synthetic carriers is an essential and important strategy for drug delivery in vivo. However, exogenous properties make synthetic nanosystems invaders that easily trigger the passive immune clearance mechanism, increasing the retention effect caused by the reticuloendothelial systems and bioadhesion, finally leading to low therapeutic efficacy and toxic effects. Recently, a cell membrane cloaking technique has been reported as a novel interfacing approach from the biological/immunological perspective, and has proved useful for improving the performance of synthetic nanocarriers in vivo. After cell membrane cloaking, nanoparticles not only acquire the physiochemical properties of natural cell membranes but also inherit unique biological functions due to the presence of membrane-anchored proteins, antigens, and immunological moieties. The derived biological properties and functions, such as immunosuppressive capability, long circulation time, and targeted recognition integrated in synthetic nanosystems, have enhanced their potential in biomedicine in the future. Here, we review the cell membrane-covered nanosystems, highlight their novelty, introduce relevant biomedical applications, and describe the future prospects for the use of this novel biomimetic system constructed from a combination of cell membranes and synthetic nanomaterials.
Genetic code restoration by artificial RNA editing of Ochre stop codon with ADAR1 deaminase
Authors: Bhakta Sonali , Azad Md Thoufic Anam , Tsukahara Toshifumi 
Published: March 2019

Abstract
Site directed mutagenesis is a very effective approach to recode genetic information. Proper linking of the catalytic domain of the RNA editing enzyme adenosine deaminase acting on RNA (ADAR) to an antisense guide RNA can convert specific adenosines (As) to inosines (Is), with the latter recognized as guanosines (Gs) during the translation process. Efforts have been made to engineer the deaminase domain of ADAR1 and the MS2 system to target specific A residues to restore G→A mutations. The target consisted of an ochre (TAA) stop codon, generated from the TGG codon encoding amino acid 58 (Trp) of enhanced green fluorescent protein (EGFP). This system had the ability to convert the stop codon (TAA) to a readable codon (TGG), thereby restoring fluorescence in a cellular system, as shown by JuLi fluorescence and LSM confocal microscopy. The specificity of the editing was confirmed by polymerase chain reaction-restriction fragment length polymorphism, as the restored EGFP mRNA could be cleaved into fragments of 160 and 100 base pairs. Direct sequencing analysis with both sense and antisense primers showed that the restoration rate was higher for the 5′ than for the 3′A. This system may be very useful for treating genetic diseases that result from G→A point mutations. Successful artificial editing of RNA in vivo can accelerate research in this field, and pioneer genetic code restoration therapy, including stop codon read-through therapy, for various genetic diseases.
Outpatient Physical Therapist Attitudes Toward and Behaviors in Cardiovascular Disease Screening: A National Survey
Authors: Severin Richard , Wang Edward , Wielechowski Adam , Phillips Shane A 
Published: March 2019

Abstract

Background
Screening the cardiovascular system is an important and necessary component of the physical therapist examination to ensure patient safety, appropriate referral, and timely medical management of cardiovascular disease (CVD) and risk factors. The most basic screening includes a measurement of resting blood pressure (BP) and heart rate (HR). Previous work demonstrated that rates of BP and HR screening and perceptions toward screening by physical therapists are inadequate.

Objective
The purpose was to assess the current attitudes and behaviors of physical therapists in the United States regarding the screening of patients for CVD or risk factors in outpatient orthopedic practice.

Design
This was a cross-sectional, online survey study.

Methods
Data were collected from an anonymous adaptive online survey delivered via an email list.

Results
A total of 1812 surveys were included in this analysis. A majority of respondents (n = 931; 51.38%) reported that at least half of their current caseload included patients either with diagnosed CVD or at moderate or greater risk of a future occurrence. A total of 14.8% of respondents measured BP and HR on the initial examination for each new patient. The most commonly self-reported barriers to screening were lack of time (37.44%) and lack of perceived importance (35.62%). The most commonly self-reported facilitators of routine screening were perceived importance (79.48%) and clinic policy (38.43%). Clinicians who managed caseloads with the highest CVD risk were the most likely to screen.

Limitations
Although the sampling population included was large and representative of the profession, only members of the American Physical Therapy Association Orthopaedic Section were included in this survey.

Conclusions
Despite the high prevalence of patients either diagnosed with or at risk for CVD, few physical therapists consistently included BP and HR on the initial examination. The results of this survey suggest that efforts to improve understanding of the importance of screening and modifications of clinic policy could be effective strategies for improving rates of HR and BP screening.
STATISTICAL ESTIMATION OF RADIOLOGICAL HAZARDS FROM RADIONUCLIDE CONTENTS IN BUILDING MATERIALS
Authors: Adewoyin O O , Omeje M , Joel E S , Odetunmibi O A 
Published: March 2019

Abstract
Thirteen (13) types of building tiles and Sharp sand commonly used for building purposes were collected for their radionuclide contents analysis using gamma ray spectrometry. The average value of the Radium equivalent activity is 208.81 Bq/kg for all samples considered was found to be lower than the recommended safe limit of 370 Bq/kg. Similarly, both external and internal hazard indices (Hex and Hin) were noted to have mean values of 0.57 and 0.73, respectively, which were lower than the recommended safe standard of 1. More so, the average absorbed gamma dose rate was observed to be 94.31 nGy/h, much higher than the recommended safe limit of 59 nGy/h.
Impaired Subcortical Detection of Auditory Changes in Schizophrenia but Not in Major Depression
Authors: Gaebler Arnim Johannes , Zweerings Jana , Koten Jan Willem , König Andrea Anna , Turetsky Bruce I , Zvyagintsev Mikhail , Mathiak Klaus 
Published: March 2019

Abstract
The mismatch negativity is a cortical response to auditory changes and its reduction is a consistent finding in schizophrenia. Recent evidence revealed that the human brain detects auditory changes already at subcortical stages of the auditory pathway. This finding, however, raises the question where in the auditory hierarchy the schizophrenic deficit first evolves and whether the well-known cortical deficit may be a consequence of dysfunction at lower hierarchical levels. Finally, it should be resolved whether mismatch profiles differ between schizophrenia and affective disorders which exhibit auditory processing deficits as well. We used functional magnetic resonance imaging to assess auditory mismatch processing in 29 patients with schizophrenia, 27 patients with major depression, and 31 healthy control subjects. Analysis included whole-brain activation, region of interest, path and connectivity analysis. In schizophrenia, mismatch deficits emerged at all stages of the auditory pathway including the inferior colliculus, thalamus, auditory, and prefrontal cortex. In depression, deficits were observed in the prefrontal cortex only. Path analysis revealed that activation deficits propagated from subcortical to cortical nodes in a feed-forward mechanism. Finally, both patient groups exhibited reduced connectivity along this processing stream. Auditory mismatch impairments in schizophrenia already manifest at the subcortical level. Moreover, subcortical deficits contribute to the well-known cortical deficits and show specificity for schizophrenia. In contrast, depression is associated with cortical dysfunction only. Hence, schizophrenia and major depression exhibit different neural profiles of sensory processing deficits. Our findings add to a converging body of evidence for brainstem and thalamic dysfunction as a hallmark of schizophrenia.
OnabotulinumtoxinA for Simultaneous Treatment of Upper Facial Lines: Subject-Reported Satisfaction and Impact From a Phase 3 Study
Authors: Rivkin Alexander Z. , Ogilvie Patricia , Dayan Steven , Yoelin Steven G. , Weichman Barry M. , Garcia Julie K. 
Published: March 2019

BACKGROUND
Patient-reported outcomes are increasingly recognized as important measures of treatment benefit.

OBJECTIVE
To evaluate subject-reported satisfaction and impact outcomes with onabotulinumtoxinA treatment in neurotoxin-naive adults with forehead lines (FHL), glabellar lines (GL), and crow's feet lines (CFL).

METHODS
This Phase 3 study randomized 787 subjects to onabotulinumtoxinA 64 U (FHL 20 U, GL 20 U, and CFL 24 U), 40 U (FHL 20 U, GL 20 U, and CFL placebo), or placebo in double-blind Period 1. Subjects could receive up to 2 additional 64 U treatments in open-label Period 2. Patient-reported outcomes were assessed using the validated Facial Line Satisfaction Questionnaire (FLSQ) and 11-item Facial Line Outcomes (FLO-11) Questionnaire.

RESULTS
The proportion of subjects mostly or very satisfied was significantly greater with onabotulinumtoxinA 64 U and 40 U versus placebo (87.9% and 81.4% vs 3.2%; p < .0001). Responder rates on FLSQ Impact Domain, FLO-11 Items 1, 4, 5, and total score were significantly greater with onabotulinumtoxinA versus placebo on Day 30 (p < .0001). Responder rates favoring onabotulinumtoxinA in Period 1 were maintained with repeated onabotulinumtoxinA 64 U treatment in Period 2.

CONCLUSION
OnabotulinumtoxinA treatment was associated with high subject satisfaction and significant improvements in appearance-related psychological and emotional impacts.