bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elicited and pre‐existing anti‐Neu5Gc antibodies differentially affect human endothelial cells transcriptome
Authors: Le Berre Ludmilla , Danger Richard , Mai Hoa L. , Amon Ron , Leviatan Ben‐Arye Shani , Bruneau Sarah , Senage Thomas , Perreault Helene , Teraiya Milan , Nguyen Thi Van Ha , Le Tourneau Thierry , Yu Hai , Chen Xi , Galli Cesare , Roussel Jean‐Christian , Manez Rafael , Costa Cristina , Brouard Sophie , Galinanes Manuel , Harris Kristina M. , Gitelman Stephen , Cozzi Emanuele , Charreau Beatrice , Padler‐Karavani Vered , Soulillou Jean‐Paul 
Published: July 2019

AbstractHumans cannot synthesize N‐glycolylneuraminic acid (Neu5Gc) but dietary Neu5Gc can be absorbed and deposited on endothelial cells (ECs) and diet‐induced anti‐Neu5Gc antibodies (Abs) develop early in human life. While the interaction of Neu5Gc and diet‐induced anti‐Neu5Gc Abs occurs in all normal individuals, endothelium activation by elicited anti‐Neu5Gc Abs following a challenge with animal‐derived materials, such as following xenotransplantation, had been postulated. Ten primary human EC preparations were cultured with affinity‐purified anti‐Neu5Gc Abs from human sera obtained before or after exposure to Neu5Gc‐glycosylated rabbit IgGs (elicited Abs). RNAs of each EC preparation stimulated in various conditions by purified Abs were exhaustively sequenced. EC transcriptomic patterns induced by elicited anti‐Neu5Gc Abs, compared with pre‐existing ones, were analyzed. qPCR, cytokines/chemokines release, and apoptosis were tested on some EC preparations. The data showed that anti‐Neu5Gc Abs induced 967 differentially expressed (DE) genes. Most DE genes are shared following EC activation by pre‐existing or anti‐human T‐cell globulin (ATG)‐elicited anti‐Neu5Gc Abs. Compared with pre‐existing anti‐Neu5Gc Abs, which are normal component of ECs environment, elicited anti‐Neu5Gc Abs down‐regulated 66 genes, including master genes of EC function. Furthermore, elicited anti‐Neu5Gc Abs combined with complement‐containing serum down‐regulated most transcripts mobilized by serum alone. Both types of anti‐Neu5Gc Abs‐induced a dose‐ and complement‐dependent release of selected cytokines and chemokines. Altogether, these data show that, compared with pre‐existing anti‐Neu5Gc Abs, ATG‐elicited anti‐Neu5Gc Abs specifically modulate genes related to cytokine responses, MAPkinase cascades, chemotaxis, and integrins and do not skew the EC transcriptome toward a pro‐inflammatory profile in vitro.
Preparation of hybrid porcine thymus containing non‐human primate thymic epithelial cells in miniature swine
Authors: Sekijima Mitsuhiro , Sahara Hisashi , Shimizu Akira , Iwanaga Takehiro , Murokawa Takahiro , Ariyoshi Yuichi , Pomposelli Thomas , Khosravi Maharlooei Mohsen , Sykes Megan , Yamada Kazuhiko 
Published: July 2019

AbstractBackgroundWe have achieved greater than a 6‐month survival of a life‐supporting kidney co‐transplanted with a vascularized thymic graft into non‐human primates (NHPs). Although we have achieved pig‐specific unresponsiveness in vitro, immunosuppression was not able to be fully weaned. Studies in mice and humanized mice suggest that a hybrid pig thymus (Hyb‐thy)‐containing host thymic epithelial cells (TECs) can optimize intra‐thymic selection, achieving xenograft tolerance with improved reconstitution of T‐cell function.MethodsWe have tested the feasibility of the preparation of a Hyb‐thy that contains NHP TECs in the donor thymic grafts. We first prepared the Hyb‐thy in the donor pigs 2‐3 weeks before xeno‐Tx. We performed six cases of Hyb‐thy preparation in six juvenile miniature swine. Two pigs received non‐manipulated cynomolgus monkey thymic cells that were isolated from an excised atrophic thymus via injection into their thymic lobes (Group 1). The remaining four received thymic cells that were isolated from non‐atrophic thymic glands (Groups 2 and 3). Pigs in Group 2 received unmanipulated thymic cells in one thymic lobe, as well as CD2‐positive cell‐depleted TEC‐enriched cells in the contralateral lobe. Pigs in Group 3 received TEC‐enriched cells alone.ResultsAll thymus‐injected pigs received tacrolimus and rapamycin until endpoint (POD16). We detected cynomolgus monkey TEC networks in pig thymus from Groups 1 and 3, while pigs in Group 2 rejected the thymic cells. We demonstrated the preparation of Hyb‐thy in pigs using tacrolimus plus rapamycin therapy.ConclusionsOur results suggest that the enrichment of TEC from the excised NHP thymus facilitated NHP TEC engraftment in pig thymus.
Seroepidemiology and genotyping of hepatitis E virus in Singapore reveal rise in number of cases and similarity of human strains to those detected in pig livers
Authors: Wong Chui Ching , Thean Su Ming , Ng Youming , Kang Joanne Su Lin , Ng Tong Yong , Chau Man Ling , Koh Tse Hsien , Chan Kwai Peng 
Published: July 2019

AbstractHepatitis E virus (HEV) causes 20 million infections worldwide yearly, of which only about 3.3 million are symptomatic. In developed Asian countries, HEV strains detected in human sera and in food sources were genetically similar, suggesting that indigenous HEV infections may be largely food‐borne. To assess the burden of hepatitis E in Singapore, we performed a seroepidemiologic study of the infection. Additionally, we carried out HEV genotyping on archived, residual HEV IgM‐positive serum samples collected between 2014 and 2016 (n = 449), and on pig liver samples (n = 36) purchased from wet markets and supermarkets. Our study shows a rise in hepatitis E incidence (IgM) from 1.7 to 4.1 cases per 100,000 resident population from 2012 to 2016 and an increase in hepatitis E IgG positivity rate among residents from 14% in 2007 to 35% in 2016. Other findings also suggest the epidemiology of hepatitis E in Singapore has shifted, from it being mainly a disease imported from the Indian subcontinent, to one that is now increasingly prevalent in our resident population. Genotypes obtained from 143 human samples identified the majority to be genotype 3 (n = 121), 21 to be genotype 1 and one to be genotype 4. Further phylogenetic analyses suggest genotype 3a to be the cause of indigenous infections in residents, which showed genetic similarity to the genotype 3a strains detected in pig livers. This link between the strains in the majority of human samples and those in pig livers consumed by the public suggests a possible food‐borne route of HEV infection in Singapore.
Alert Correlation for Detecting Cyber-Manufacturing Attacks and Intrusions
Authors: Wu Mingtao , Moon Young B. 
Published: July 2019

Abstract
Cyber-manufacturing system (CMS) is a vision of smart factories where manufacturing processes are fully integrated with computational components. In CMS, an effective intrusion detection system (IDS) is essential in protecting manufacturing operations from cyber-physical attacks. Current IDS analyses data from cyber and physical domains but produces reports separately for cyber domain and physical domain. To utilize connections between cyber and physical alerts, this paper presents a cyber-physical alert correlation method. To evaluate the method, four case studies have been developed and carried out on a CMS testbed. The experimental results demonstrate that the method can effectively reduce the number of false alerts, improve the detection accuracy, and identify root causes.
Superhuman AI for multiplayer poker
Authors: Brown Noam , Sandholm Tuomas 
Published: July 2019

AI now masters six-player poker
Computer programs have shown superiority over humans in two-player games such as chess, Go, and heads-up, no-limit Texas hold'em poker. However, poker games usually include six players—a much trickier challenge for artificial intelligence than the two-player variant. Brown and Sandholm developed a program, dubbed Pluribus, that learned how to play six-player no-limit Texas hold'em by playing against five copies of itself (see the Perspective by Blair and Saffidine). When pitted against five elite professional poker players, or with five copies of Pluribus playing against one professional, the computer performed significantly better than humans over the course of 10,000 hands of poker.

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The rise of pinnacle reefs: Islands of diversity in seas of despair
Authors: McLaughlin Patrick I. , Bancroft Alyssa M. , Brett Carlton E. , Emsbo Poul 
Published: July 2019

ABSTRACT
Pinnacle reef tracts are geomorphic features of carbonate systems that originated in the early Silurian and display an episodic distribution into the Cenozoic. Detailed study of Silurian pinnacle reefs of the United States midcontinent demonstrates repeated motifs, but most enigmatic is the coincidence of carbonate carbon isotope (δ13Ccarb) excursions and reef pulses. Silurian δ13Ccarb excursions were associated with environmental changes and extinctions, and reefs appear to mark a resurgence of conditions favorable to biomineralizers following those extinction events. Previous workers in the region identified six discrete reef origination events in the United States midcontinent during the Silurian. Our reevaluation of outcrops and cores, conodont collections, and the generation of considerable new chemostratigraphic data across the region are clarifying the age relations of these events and their relationships to perturbations of the global carbon cycle.
Hydrodynamic role of groundwater in bolide impact: Evidence from the Kentland structure, Indiana, USA
Authors: Gutschick† Raymond C. 
Published: July 2019

ABSTRACT
The extremely important role of groundwater has been largely overlooked in studies of meteorite and comet impact processes. Beyond the radius of plasma generation, impacts can produce massive shattering in saturated porous rocks. Fluid pressure rise reduces rock strength and facilitates hydrofracture, to produce intraformational monomict breccias, faulting, and generation of mobile polymict breccia slurries. Decompression of a deep “transient” crater accounts for complex central uplift and gravitational collapse of tremendous slide blocks that in turn cause injection and ejection of fluidized breccia. As pore fluid pressures equilibrate, frictional strength increases, and the structural form is locked into stability. Evidence is reported here for Kentland, Indiana, where quarry rocks display relatively low pressure-temperature (elastic to ductile transition, 100 kb–100 °C) impact phases of the model of D. Stöffler. Breccias include monomict, polymict, mixed polymict-fault, and conventional fault types. The monomict breccias are associated with aquifer beds and formed by pervasive shockwave transmission on impact. Polymict breccias are derived from all rock types and formed from late stage injection-ejection pseudoviscous slurries. These processes can apply to similar impacts like Wells Creek, Flynn Creek, Decaturville, Sierra Madre, and many others.
Screening for early onset neonatal sepsis: NICE guidance-based practice versus projected application of the Kaiser Permanente sepsis risk calculator in the UK population
Authors: Goel Nitin , Shrestha Sudeep , Smith Rhian , Mehta Akshay , Ketty Malini , Muxworthy Helen , Abelian Artur , Kirupaalar Vickness , Saeed Shakir , Jain Shikha , Asokkumar Amar , Natti Murali , Barnard Ian , Pitchaikani Prem Kumar , Banerjee Sujoy 
Published: July 2019

ObjectiveTo compare management recommendations of the National Institute for Health and Care Excellence (NICE) guidelines with the Kaiser Permanente sepsis risk calculator (SRC) for risk of early onset neonatal sepsis (EONS).DesignMulticentre prospective observational projection study.SettingEight maternity hospitals in Wales, UK.PatientsAll live births ≥34 weeks gestation over a 3-month period (February–April 2018).MethodsDemographics, maternal and infant risk factors, infant’s clinical status, antibiotic usage and blood culture results from first 72 hours of birth were collected. Infants were managed using NICE recommendations and decisions compared with that projected by SRC.Main outcome measureProportion of infants recommended for antibiotics on either tool.ResultsOf 4992 eligible infants, complete data were available for 3593 (71.9%). Of these, 576 (16%) were started on antibiotics as per NICE recommendations compared with 156 (4.3%) projected by the SRC, a relative reduction of 74%. Of the 426 infants avoiding antibiotics, SRC assigned 314 (54.6%) to normal care only. There were seven positive blood cultures—three infants were recommended antibiotics by both, three were not identified in the asymptomatic stage by either; one was a contaminant. No EONS-related readmission was reported.ConclusionThe judicious adoption of SRC in UK clinical practice for screening and management of EONS could potentially reduce interventions and antibiotic usage in three out of four term or near-term infants and promote earlier discharge from hospital in >50%. We did not identify any EONS case missed by SRC when compared with NICE. These results have significant implications for healthcare resources.
Physically active individuals have a 23% lower risk of any colorectal neoplasia and a 27% lower risk of advanced colorectal neoplasia than their non-active counterparts: systematic review and meta-analysis of observational studies
Authors: Wang Jingjing , Huang Liwen , Gao Yang , Wang Yanhong , Chen Shanquan , Huang Junjie , Zheng Wenjing , Bao Pingping , Gong Yangming , Zhang Yanfeng , Wang Mei , Wong Martin Chi Sang 
Published: July 2019

BackgroundFew studies have examined the associations between physical activity (PA), sedentary behaviour (SB) and risk of colorectal neoplasia (CN).MethodsWe systematically searched Medline, Embase, PsyInfo, Cochrane and other sources from their inception to 30 September 2018 for cohort, case-control and cross-sectional studies that evaluated these associations in asymptomatic, average-risk subjects. Random-effect models were used to estimate relative risks (RRs) of any-type CN, advanced CN, and non-advanced CN, respectively, in individuals with the highest versus the lowest level of PA and SB. Dose-response analyses and subgroup analyses were conducted. The I2statistic was used to examine heterogeneity among studies.ResultsWe identified 32 observational studies, including 17 cross-sectional studies, 10 case-control studies and five longitudinal studies. PA (highest vs lowest) was inversely associated with risk for any-type CN (n=23 studies) and advanced CN (n=15 studies), with a RR of 0.77 (95% CI=0.71 to 0.83, I2=57.5%) and 0.73 (95% CI=0.63 to 0.82, I2=45.5%), respectively. There was no association between PA and non-advanced CN (n=5 studies). There was an as association between PA and any-type CN in both sexes, and also for the distal colon. We found no dose–response relationship between PA and any-type or advanced CN. Based on three studies identified, SB time (longest vs shortest) was associated with an increased risk of advanced CN (RR=1.24, 95% CI 1.04 to 1.49, I2=14.4%). No publication bias was detected by Begg’s test.ConclusionWe report a 23% lower relative risk of any type of CN and a 27% lower risk of advanced CN in people with the highest level of PA compared with those in the lowest.
International Study of Definitions of English-Language Terms for Suicidal Behaviours: protocol of an opinion survey
Authors: Goodfellow Benjamin , Kõlves Kairi , De Leo Diego , Silverman Morton , Berman Alan , Mann John , Arensman Ella , Hawton Keith , Phillips Michael , Vijayakumar Lakshmi 
Published: July 2019

IntroductionThe objective of present paper is to outline the methodology of the International Study of Definitions of English-Language Terms for Suicidal Behaviours (ISDELTSB). The aim of the study is to survey existing English language terms and definitions used around the world for suicidal behaviour.Methods and analysisThe ISDELTSB is a worldwide survey based on one ‘designated expert’ per each WHO-registered country. ‘Experts’ were contacted through the International Association for Suicide Prevention (IASP), the World Psychiatric Association and the World Organization of Family Doctors. Each individual was sent an invitation to participate and a link to an online questionnaire. A comparison sample was created by inviting all IASP members to respond to the questionnaire. The questionnaire was designed to assess respondents’ preferences about a particular set of terms and definitions by using the four major criteria of the definition of suicide identified in the literature (outcome, intent, knowledge and agency). The questionnaire used a multiple-choice question format. Participants were asked to choose one term in the list for each of the proposed definitions. Statements and definitions in the questionnaire were elaborated using the four main features of the definition of suicide, starting by the definitions and terms for which there is already a certain degree of consensus and then progressing to definitions and terms less agreed on.Ethics and disseminationThe study protocol obtained approval of Griffith University’s Ethics Committee (ethics reference number 2017/601) and in accordance with the Australian National Statement on Ethical Conduct in Human Research. Respondents are asked if they accept to be personally acknowledged in any output originating from this study, and if so to provide their full name, title and affiliations. If respondents do not accept, they are informed that the conduct of this research respects Griffith University’s Privacy Plan and that identified personal information is confidential and that anonymity will at all times be safeguarded. As detailed in the questionnaire cover letter, by answering the online or paper version of the questionnaire, respondents express their consent to participate. Dissemination of results will be done through a peer-reviewed journal article publication. This study aims to map the international use of definitions and terms for suicidal behaviour and ideation and favour the future use of an internationally shared set of terms and definitions. This will hopefully avoid undue duplication of efforts and reliably permit meta-analysis of data produced in different countries.
Retrospective study of prognosis and relating factors of cardiac complications associated with electrical injuries at a single centre in Korea
Authors: Choi Jae Hyuk , Han Donghoon , Kang Si-Hyuck , Yoon Chang-Hwan , Cho Jung Rae , Kym Dohern 
Published: July 2019

ObjectivesTo date, no research has investigated the association between cardiac complication and electrical injury; hence, we aimed to assess the consequences and relating factors of cardiac complications from electrical injuries in South Korea.DesignRetrospective single-centre study.Participants721 patients who had electrical injury–related admission during 2007–2017. An electronic medical record system was used to extract records of patients admitted for electrical injury treatment.ResultsCardiac complications included abnormal parameters of myocardial damage, abnormal regional wall motion detected via echocardiogram, dysrhythmia (eg, bradycardia, atrial flutter/fibrillation) and ventricular tachycardia or fibrillation. Overall, 107 patients (14.8%) experienced cardiac complications. The average admission duration and intensive care unit stay duration were significantly longer in patients with cardiac complications than in those without them (75.0±45.3 vs 56.6±48.0 days and 19.3±24.1 vs 10.4±15.5 days, respectively, p<0.01 for both). Of the total cardiac cases, 72.9% had Troponin I elevation, 3.7% had regional wall motion abnormality, and 5.6% had atrial flutter/fibrillation. Overall, seven patients from the cardiac complication group and three patients from the control group died (p=0.01). All deaths occurred within 32 days, and the most common cause of death was septic shock. Total body surface area (TBSA) was only positively related factor to cardiac complications.ConclusionThis study is the first in South Korea to reveal that electrical accident patients with cardiac complications experience poorer in-hospital prognosis, and TBSA was the only risk factor of cardiac complications. And initial treatment for infection and inflammations could be important in electrical injury.
A multifaceted intervention to improve the quality of care for patients undergoing total joint arthroplasty
Authors: Sorensen Lyle , Idemoto Lori , Streifel Janet , Williams Barbara , Mecklenburg Robert , Blackmore Craig 
Published: July 2019

Knee and hip arthroplasties vary in cost, quality and outcomes. We developed a Lean quality improvement intervention for knee and hip arthroplasty patients encompassing the recognition, readiness, restoration and recovery phases of care.The intervention included standardised, evidence-based pathways, shared decision making, patient and family member engagement, and transdisciplinary rounding, implemented successively through a series of rapid process improvement workshops. We evaluated the intervention through run charts and time series analysis for 2005–2014. Outcomes included length of stay (LOS), 30-day readmission, discharge disposition, postsurgical complications and patient satisfaction.Included were 4253 total joint arthroplasty procedures, 1659 hip and 2594 knee. LOS decreased from 3.2 to 2.4 days postintervention for both hip and knee patients (p<0.001). The 30-day hospital readmission rate for hip patients decreased from 3.1% (18/576) to 1.1% (5/446, p=0.032) with knee patients unchanged. Discharge to home (vs rehabilitation facility or skilled nursing facility) increased from 72% (415/576) to 91% (405/446) (p<0.001) for hip patients, and from 70% (599/860) to 87% (578/663) for knee patients (p<0.001).Our standardised multifaceted Lean quality improvement programme was associated with reduced LOS, decreased readmission rates and improved discharge disposition in total knee and hip arthroplasty patients.
Rehoming a surrendered dog to its original owner
Authors: Cheung Wesley , Fawcett Anne 
Published: July 2019

THIS series gives readers the opportunity to consider and contribute to discussion of some of the ethical dilemmas that can arise in veterinary practice. Each month, a case scenario is presented, followed by discussion of some of the issues involved.In addition, a possible way forward is suggested; however, there is rarely a cut‐and‐dried answer in such cases, and readers may wish to suggest an alternative approach. This month's dilemma, ‘Rehoming a surrendered dog to its original owner’, was submitted and is discussed by Wesley Cheung and Anne Fawcett. Readers with comments to contribute are invited to send them as soon as possible, so that they can be considered for publication in the next issue.The series is being coordinated by Steven McCulloch, senior lecturer in human animal studies at the Centre for Animal Welfare, University of Winchester. It aims to provide a framework that will help practising veterinarians find solutions when facing similar dilemmas.