bluuebunny/crossref_metadata_2025_split huggingface dataset:
Video intervention for the psychiatric waiting room: proof-of-concept randomised controlled trial of RESOLVE (Relaxation Exercise, SOLVing problem and cognitive Errors)
Authors: Naeem Farooq , Munshi Tariq , Gratzer David , Rodie David , Irfan Muhammad , Rao Sanjay , Husain Nusrat , Farooq Saeed , Sanches Marcos , Ayub Muhammad , Lecomte Tania 
Published: September 2019

Background
The waiting room in psychiatric services can provide an ideal setting for offering evidence-based psychological interventions that can be delivered through electronic media. Currently, there is no intervention available that have been developed or tested in mental health.

Aims
This proof-of-concept study aimed to evaluate a pilot design of RESOLVE (Relaxation Exercise, SOLving problem and cognitiVe Errors) to test the procedure and obtain outcome data to inform future, definitive trials (trial registration at Clinicaltrials.gov NCT02536924, REB Number: PSIY-477-15).

Method
Forty participants were enrolled and equally randomised to the intervention, RESOLVE plus treatment as usual arm (TAU), or to a control group (TAU only). Those in the intervention group watched RESOLVE in a room adjacent to the waiting area. Participants in the control received routine care. Outcome measures included the Hospital Anxiety and Depression Scale; the Clinical Outcomes in Routine Evaluations outcome measure; and the World Health Organization Disability Assessment Schedule. These measures were performed by a masked assessor at baseline and at 6-week follow-up. Additionally, we measured the number of contacts with mental health services during the prior 4 weeks. Both intention-to-treat and per protocol analyses were performed.

Results
The study proved feasible. We were able to recruit the required number of participants. There was a statistically significant improvement in depression (P < 0.001), anxiety (P < 0.001), general psychopathology (P < 0.001) and disability (P = 0.0361) in favour of the intervention group. People in the intervention group were less likely to contact the service (P = 0.012) post-intervention.

Conclusions
Findings provide preliminary evidence that evidence-based psychosocial interventions can be delivered through electronic media in a waiting-room setting. The outcome data from this study will be used for future definitive trials.

Declaration of interest
None.
CBE1 is a manchette and mitochondria associated protein with a potential role in somatic cell proliferation
Authors: Pleuger Christiane , Lehti Mari S , Cooper Madeleine , O’Connor Anne E , Merriner D Jo , Smyth Ian M , Cottle Denny L , Fietz Daniela , Bergmann Martin , O’Bryan Moira K 
Published: September 2019

Abstract
Ciliated bronchial epithelium 1 (CBE1) is a microtubule-associated protein localized to the manchette and developing flagellum during spermiogenesis, and associated with sperm maturation arrest in humans. It was hypothesized that CBE1 functions in microtubule-mediated transport mechanisms and sperm tail formation. To test this hypothesis, we analysed Cbe1 expression and localization during spermiogenesis, and in mouse IMCD3 cells as a model of ciliogenesis. Further, we generated and analysed the fertility of a Cbe1 mutant mouse line. Mice containing a homozygous deletion in the long forms of Cbe1 were born at a lower frequency than predicted by Mendelian inheritance, however, adult male mice were fertile. An in-depth analysis of the Cbe1 gene revealed alternative transcript variants, which were not affected by the exon 2 mutation. To assess whether short variants compensate for the loss of long variants, exons 2 and 4 (which affect all variants) were individually mutated in IMCD3 cells and the effect on cell proliferation and ciliogenesis analysed. In wild type IMCD3 cells, both variants were upregulated during cilia assembly. CBE1 protein was not a structural component of cilia, rather, CBE1 localised to the mitochondria and the contractile ring of dividing IMCD3 cells. While IMCD3 cells carrying the mutation in long variants showed no phenotypic alterations, the mutation in exon 4 resulted in a significantly decreased proliferation rate. This study reveals that long isoforms of CBE1 are not essential for male fertility. Data, however, suggests that CBE1 is associated to intra-manchette transport and mid-piece formation of the sperm tail.
Quality of Recovery After Breast Surgery: A Multicenter Randomized Clinical Trial Comparing Pectoral Nerves Interfascial Plane (Pectoral Nerves II) Block With Surgical Infiltration
Authors: Barrington Michael J. , Seah Gloria J. , Gotmaker Robert , Lim Daniel , Byrne Kelly 
Published: September 2019

BACKGROUND:
Pectoral nerves (PECS II) block is a popular regional analgesia technique for breast surgery. PECS II block or local infiltration by surgeon may improve outcomes including quality of recovery (QoR).

METHODS:
In this multicenter randomized clinical trial, 104 female patients undergoing breast surgery received: (1) PECS II block with local anesthetic and surgical infiltration with 0.9% saline (PECS group) or (2) PECS II block with 0.9% saline and surgical infiltration with local anesthetic (infiltration group). Patients, anesthetists, surgeons, nursing staff, and research assistants were blinded to group allocation. Patients received standardized general anesthesia and multimodal analgesia. The primary outcome was the global score (maximum score, 150; good recovery, 118) of the multidimensional (pain, comfort, independence, psychological, emotional) QoR-15 questionnaire measured 24 hours postoperatively. Secondary outcomes were pain, and its functional interference measured 24 hours and 3 months postoperatively using the Brief Pain Inventory (BPI) short form (0, optimal; 120, worst possible). Randomly assigned groups were compared on outcomes using the Wilcoxon rank-sum test, and the results were reported as median difference with 95% confidence interval.

RESULTS:
One hundred eight patients were recruited from August 17, 2016 to June 8, 2018, and 4 patients were withdrawn. Twelve patients from 104 had mastectomy, with the remainder having less invasive surgery. Baseline QoR-15 global scores reported as median [quartiles] were 135 [129, 143] in the PECS group and 139 [127, 143] in the infiltration group. The 24-hour QoR-15 global score reported as median [quartiles] was 131 [116, 140] in the PECS group and 123 [117, 143] in the infiltration group (P = .60), with median difference (95% confidence interval) of −2 (−9 to 5). The median difference reported as infiltration minus PECS for QoR-15 domains was pain 0 (−2 to 1), physical comfort −1 (−3 to 2), physical independence 0 (−2 to 1), psychological support 0 (0–0), and emotions 0 (−1 to 2) (P > .28). The BPI pain subscale at 24 hours (0–40, lower score indicates less pain), reported as median [quartiles], was 7 [2, 13] in the PECS group and 10 [5, 17] in the infiltration group (P = .15). The BPI global score at 24 hours, reported as median [quartiles], was 20 [7, 36] in the PECS group and 23 [10, 43] in the infiltration group (P = .34) and at 3 months was 0 [0, 14] and 0 [0, 11] (P = .85).

CONCLUSIONS:
After mostly minor surgery for breast cancer, PECS II block was not superior to local infiltration by the surgeon.
Experimental models and tools to tackle glioblastoma
Authors: Robertson Faye L. , Marqués-Torrejón Maria-Angeles , Morrison Gillian M. , Pollard Steven M. 
Published: September 2019

ABSTRACT
Glioblastoma multiforme (GBM) is one of the deadliest human cancers. Despite increasing knowledge of the genetic and epigenetic changes that underlie tumour initiation and growth, the prognosis for GBM patients remains dismal. Genome analysis has failed to lead to success in the clinic. Fresh approaches are needed that can stimulate new discoveries across all levels: cell-intrinsic mechanisms (transcriptional/epigenetic and metabolic), cell-cell signalling, niche and microenvironment, systemic signals, immune regulation, and tissue-level physical forces. GBMs are inherently extremely challenging: tumour detection occurs too late, and cells infiltrate widely, hiding in quiescent states behind the blood-brain barrier. The complexity of the brain tissue also provides varied and complex microenvironments that direct cancer cell fates. Phenotypic heterogeneity is therefore superimposed onto pervasive genetic heterogeneity. Despite this bleak outlook, there are reasons for optimism. A myriad of complementary, and increasingly sophisticated, experimental approaches can now be used across the research pipeline, from simple reductionist models devised to delineate molecular and cellular mechanisms, to complex animal models required for preclinical testing of new therapeutic approaches. No single model can cover the breadth of unresolved questions. This Review therefore aims to guide investigators in choosing the right model for their question. We also discuss the recent convergence of two key technologies: human stem cell and cancer stem cell culture, as well as CRISPR/Cas tools for precise genome manipulations. New functional genetic approaches in tailored models will likely fuel new discoveries, new target identification and new therapeutic strategies to tackle GBM.
SH3YL1 cooperates with ESCRT-I in the sorting and degradation of the EGF receptor
Authors: Hasegawa Junya , Jebri Imen , Yamamoto Hikaru , Tsujita Kazuya , Tokuda Emi , Shibata Hideki , Maki Masatoshi , Itoh Toshiki 
Published: September 2019

ABSTRACT
Ubiquitinated membrane proteins such as epidermal growth factor receptor (EGFR) are delivered to early endosomes and then sorted to lysosomes via multivesicular bodies (MVBs) for degradation. The regulatory mechanism underlying formation of intralumenal vesicles en route to generation of MVBs is not fully understood. In this study, we found that SH3YL1, a phosphoinositide-binding protein, had a vesicular localization pattern overlapping with internalized EGF in endosomes in the degradative pathway. Deficiency of SH3YL1 prevents EGF trafficking from early to late endosomes and inhibits degradation of EGFR. Moreover, we show that SH3YL1 mediates EGFR sorting into MVBs in a manner dependent on its C-terminal SH3 domain, which is necessary for the interaction with an ESCRT-I component, Vps37B. Taken together, our observations reveal an indispensable role of SH3YL1 in MVB sorting and EGFR degradation mediated by ESCRT complexes.
Tetraspanin CD151 and integrin α3β1 contribute to the stabilization of integrin α6β4-containing cell-matrix adhesions
Authors: te Molder Lisa , Juksar Juri , Harkes Rolf , Wang Wei , Kreft Maaike , Sonnenberg Arnoud 
Published: September 2019

ABSTRACT
Tetraspanin CD151 has been suggested to regulate cell adhesion through its association with laminin-binding integrins α3β1 and α6β4; however, its precise function in keratinocyte adhesion remains elusive. In this study, we investigated the role of CD151 in the formation and maintenance of laminin-associated adhesions. We show that CD151, through binding to integrin α3β1, plays a critical role in the stabilization of an adhesion structure with a distinct molecular composition of hemidesmosomes with tetraspanin features. These hybrid cell-matrix adhesions, which are formed early during cell adhesion and spreading and at later stages of cell spreading, are present in the central region of the cells. They contain the CD151–α3β1/α6β4 integrin complexes and the cytoskeletal linker protein plectin, but are not anchored to the keratin filaments. In contrast, hemidesmosomes, keratin filament-associated adhesions that contain integrin α6β4, plectin, BP180 (encoded by COL17A1) and BP230 (encoded by DST), do not require CD151 for their formation or maintenance. These findings provide new insights into the dynamic and complex regulation of adhesion structures in keratinocytes and the pathogenic mechanisms underlying skin blistering diseases caused by mutations in the gene for CD151.
An Azulene-Based Chiral Helicene and Its Air-Stable Cation Radical
Authors: Narita Masahiro , Teraoka Takaaki , Murafuji Toshihiro , Shiota Yoshihito , Yoshizawa Kazunari , Mori Shigeki , Uno Hidemitsu , Kanegawa Shinji , Sato Osamu , Goto Kenta , Tani Fumito 
Published: September 2019

Abstract
A helicene compound called AIBTh, wherein two azulene units are fused with isobenzothiophene, has been prepared and characterized by spectroscopic and crystallographic methods. The enantiomers of AIBTh were resolved by HPLC, exhibiting stable optical activity. AIBTh showed two reversible oxidation and one irreversible reduction waves with a HOMO-LUMO gap of 2.07 eV. Upon one-electron oxidation of AIBTh, its air-stable cation radical was isolated and analyzed by EPR as well as X-ray crystallography. Based on the EPR spectrum, the crystal structure, and DFT calculation, it is suggested that favorable resonance structures including aromatic tropylium cation forms and wide delocalization of electronic spin are dominating in the electronic structure of the cation radical.
Treatment optimisation for hepatitis C in the era of combination direct-acting antiviral therapy: a systematic review and meta-analysis
Authors: Jones Christopher R. , Flower Barnaby F. , Barber Ella , Simmons Bryony , Cooke Graham S. 
Published: September 2019

Background: Prior to direct-acting antiviral (DAA) therapy, personalised medicine played an important role in the treatment of hepatitis C virus (HCV). Whilst simplified treatment strategies are central to treatment scale-up, some patients will benefit from treatment optimisation. This systematic review and meta-analysis explores treatment optimisation strategies in the DAA era. Methods: We systematically searched Medline, Embase, and Web of Science for studies that adopted a stratified or personalised strategy using a licensed combination DAA regimen, alone or with additional agents. We performed a thematic analysis to classify optimisation strategies and a meta-analysis of sustained virologic response rates (SVR), exploring heterogeneity with subgroup analyses and meta-regression. Results: We included 64 studies (9450 participants). Thematic analysis found evidence of three approaches: duration, combination, and/or dose optimisation. We separated strategies into those aiming to maintain SVR in the absence of predictors of failure, and those aiming to improve SVR in the presence of predictors of failure. Shortened duration regimens achieve pooled SVR rates of 94.2% (92.3-95.9%) for 8 weeks, 81.1% (75.1-86.6%) for 6 weeks, and 63.1% (39.9-83.7%) for ≤4 weeks. Personalised strategies (100% vs 87.6%; p<0.001) and therapy shortened according to ≥3 host/viral factors (92.9% vs 81.4% or 87.2% for 1 or 2 host/viral factors, respectively; p=0.008) offer higher SVR rates when shortening therapy. Hard-to-treat HCV genotype 3 patients suffer lower SVR rates despite treatment optimisation (92.6% vs 98.2%; p=0.001). Conclusions: Treatment optimisation for individuals with multiple predictors of treatment failure can offer high SVR rates. More evidence is needed to identify with confidence those individuals in whom SVR can be achieved with shortened duration treatment.
SOPA, FAMÍLIA E TRABALHO: PERCEPÇÕES CONSTITUÍDAS ENTRE FORQUETA E ITÁLIA
Authors: Prodanov Cleber Cristiano , Magalhães Magna Lima , Metz Rogério De Vargas 
Published: September 2019

Resumo: Este estudo aborda as percepções de moradores da zona rural de Forqueta, distrito de Caxias do Sul, sobre o alimento. A localidade está inserida na região historicamente conhecida pela imigração italiana do século XIX e integra o roteiro turístico da região serrana do Rio Grande do Sul. Buscou-se, com base na narrativa oral, o recordar de sujeitos que vivenciaram experiências relacionadas à gastronomia italiana e que acionam, a partir de suas lembranças e rememorações, códigos culturais relacionados aos alimentos. Entende-se, de acordo com Alessandro Portelli (1996), que as narrativas apresentam significados individuais relacionados à vivência e à subjetividade de cada indivíduo. Para tanto, lança-se mão da história oral como metodologia de trabalho, o que possibilita conhecer e registrar diferentes manifestações socioculturais. Destacam-se, portanto, no artigo, as percepções e memórias de três descendentes de italianos que viajaram e conheceram o país de seus antepassados, a Itália. Entende-se o alimento como código cultural constituído em uma complexidade histórica e que pode ser relacionado à constituição de identidades e autorrepresentação.Palavras-chave: Alimento. Memória. Italianos. Imigração.Abstract: This study approaches the perceptions of residents of Forqueta rural area, Caxias do Sul district, about food. The locality is inserted in the region historically known by the Italian immigration of century XIX and it integrates the itinerary that is in the tourist route of the mountainous region of Rio Grande do Sul. Based on the oral narrative, we sought to recall the subjects who experienced experiences related to the Italian gastronomy and that trigger, from their memories and recollections, cultural codes related to food. According to Alessandro Portelli (1996), it is possible to comprehend that the narratives show individual meanings related to each person experience and subjectivity. Therefore, it was used the oral history as this works methodology, that enables us to know and to register different sociocultural manifestations. Stands out the perceptions and memories of three Italian descendants who had traveled and visited their parent’s country, Italy. The foods are understood as a cultural code that was made during a historical complexity and can be related to identity construction and self-representation. Keywords: Foods. Memory. Italians. Immigration.
Managing women in pregnancy after bariatric surgery: the midwife as the co-ordinator of care
Authors: Abraham Jenny , Neha Shah , Power Alison 
Published: September 2019

Bariatric surgery is a recommended, cost-effective, evidenced-based intervention to reduce weight and associated comorbidities in severely obese people. People with a BMI of 40 kg/m2 or more, or a BMI between 35–40 kg/m2 with other medical conditions such as diabetes, hypertension, high cholesterol and obstructive sleep apnoea meet the criteria to be considered for bariatric surgery. Over the past 10 years, bariatric surgery in the UK has been more widely accessible and consequently midwives may be required to care for pregnant women who have undergone bariatric surgery such as a gastric band, sleeve gastrectomy and gastric bypass. Midwives are required to work co-operatively, recognising and working within the limits of their competence and providing leadership. The aim of this article is to consider the midwife's role as co-ordinator of care for pregnant women who have undergone bariatric surgery. It outlines the most common bariatric procedures and specific considerations, including nutritional supplementation required when providing care to women in the antenatal and postnatal period.
200  Gbit/s/λ PDM-PAM-4 PON system based on intensity modulation and coherent detection
Authors: Zhang Jiao , Yu Jianjun , Li Xinying , Wang Kaihui , Zhou Wen , Xiao Jiangnan , Zhao Li , Pan Xiaolong , Liu Bo , Xin Xiangjun 
Published: September 2019

A low-complexity and cost-efficient coherent detection-based 100 Gb/s or beyond passive optical network (PON) has attracted a lot of attention in recent years, as this technology offers high receiver sensitivity, colorless frequency selectivity, and linear detection enabling channel impairment compensation in the digital domain. We experimentally demonstrate the first single-wavelength 200 Gb/s coherent PON over 20 km downstream transmission with polarization division multiplexed four-level pulsed amplitude modulation (PDM-PAM-4) signals in the C-band. The intensity modulator replaces the costly in-phase/quadrature modulator, and hardware-efficient carrier recovery technologies are used for recovery of PAM-4 symbols. By using optimized Nyquist pulse shaping, the transceiver bandwidth can be reduced to within 50 GHz. A maximum power budget of 32.5 dB can be achieved for 200 Gb/s/λ PDM-PAM-4 at a bit error rate of

1
×

10

−
2

over 20 km fiber transmission. The possibility of a 200 Gb/s/λ PON is investigated using intensity modulation and coherent detection for the first time to our knowledge.
Malignant Granular Cell Tumor of the Bile Duct
Authors: Quinn Patrick L. , Abdelfatah Eihab , Galan Mark A. , Ahlawat Sushil K. , Chokshi Ravi J. 
Published: September 2019

ABSTRACT
A 56-year-old man presented to the emergency department with painless jaundice and weight loss. Abdominal ultrasound detected dilation of the common bile duct and the intrahepatic bile ducts. Follow-up with endoscopic retrograde cholangiography exposed a stricture of the common hepatic duct, with cholangioscopy identifying an infiltrating tumor. Biopsy revealed a granular cell tumor, which was confirmed by positive S-100 immunohistochemical staining. Surgical excision confirmed granular cell tumor of the bile duct with morphological features suggestive of malignancy.
Studies on Wall-Slip in Entangled Polymeric Liquids
Authors: Joshi Yogesh M. 
Published: September 2019

Abstract
In this work, it is attempted to theoretically understand the phenomenon of wall slip through empirical and molecular models. Initially, we use the framework a transient network theory. We show that the severe disentanglement in the interfacial region can give rise to non-monotonic flow curve locally in that region. Further, we generalize this model into a unified slip model, which predicts wall slip by either disentanglement or by debonding mechanism, depending upon the adhesive energy of the wall-polymer pair. The model predictions of the critical wall shear stress are in good agreement with experiments for various adhesive energies of the wall-polymer pair. The model predicts that the temperature dependence of the critical wall shear stress for debonding is different than that of disentanglement mechanism under certain experimental conditions. To validate the predictions of unified model, we measure the critical stress for sudden slip due to debonding for various temperatures using cone and plate viscometer with fluoroelastomer-coated cone. The temperature dependence of the critical stress for instability (slip) on a coated cone is found out to be inversely dependent on temperature, which expected for the case of debonding. In the final part of this thesis, we develop a parameter-free tube model for predicting the stick-slip phenomenon. The model, which is based on the contour variable model [Mead et al., 1998, Macromolecules, 31, 7895], considers the dynamics of the tethered chains, which are grafted on a high-energy wall and which are entangled with the bulk chains flowing past them. We show that the restricted relaxation modes of the tethered molecule give rise to discontinuous slip instability. More specifically, the slow relaxation of the tethered chain due to the restricted convective constraint release is unable to randomize its flow-induced orientation above a critical shear rate or stress. This decreases the resistance to flow for the bulk chains, which suddenly slip past the oriented tethered chains.