bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation of a magnetic graphene/polydopamine nanocomposite for magnetic dispersive solid-phase extraction of benzoylurea insecticides in environmental water samples
Authors: Huang Xiaodong , Qiao Kexin , Li Lingyun , Liu Guangyang , Xu Xiaomin , Lu Runhua , Gao Haixiang , Xu Donghui 
Published: June 2019

AbstractA magnetic graphene/polydopamine (MG/PDA) nanocomposite has been prepared and used as sorbent for magnetic dispersive solid-phase extraction (MDSPE) of four benzoylurea insecticides in environmental water samples. The obtained nanocomposites were characterized by transmission electron microscopy, scanning electron microscopy, vibrating sample magnetometry, powder X-ray diffraction, fourier transform infrared spectroscopy, surface area and porosity analysis and thermogravimetric analysis. To investigate the adsorption performance of MG/PDA for target analytes, various parameters affecting the MG/PDA-based MDSPE procedure were optimized. Under the optimal conditions, the established method exhibits good linearity (R2 ≥ 0.9988) in the concentration range 2.5–500 µg L−1. A low limit of detection (0.75 µg L−1, signal/noise = 3:1), a low limit of quantification (2.50 µg L−1, signal/noise = 10:1), and good precision (intraday relative standard deviation ≤3.6%, interday relative standard deviation ≤4.5%) are also achieved. Finally, the simple, fast, and sensitive sample preparation technique was successfully used to determine benzoylurea insecticides in environmental water samples.
Lossy mode resonance sensors based on lateral light incidence in nanocoated planar waveguides
Authors: Fuentes Omar , Del Villar Ignacio , Corres Jesus M. , Matias Ignacio R. 
Published: June 2019

AbstractThe deposition of an indium oxide (In2O3) thin film on conventional planar waveguides (a coverslip and a glass slide) allows generating lossy mode resonances (LMR) by lateral incidence of light on the waveguide and by registering the optical spectrum in a spectrometer. This novel sensing system becomes an alternative to optical fibre, the substrate where LMR-based sensors have been developed so far, since it is easier to handle and more robust. An additional advantage is that cost effective waveguides, such as slides or coverslips, can be used in a platform that resembles surface plasmon resonance-based sensors in the Kretschmann configuration but without the need for a coupling prism and with the advantage of being able to generate TE and TM LMR resonances with metallic oxide or polymer thin films. The results are corroborated with simulations, which provide in-depth understanding of the phenomena involved in the sensing system. As a proof-of-concept for the optical platform, two refractometers were developed, one with low sensitivity and for a wide range of refractive indices, and the other with higher sensitivity but for a narrower refractive index range. The sensors presented here open up the path for the development of LMR-based chemical sensors, environmental sensors, biosensors, or even the generation of other optical phenomena with the deposition of multilayer structures, gratings or nanostructures, which is much easier in a planar waveguide than in an optical fibre.
Frequency of the CYP2C19*17 polymorphism in a Chilean population and its effect on voriconazole plasma concentration in immunocompromised children
Authors: Espinoza N. , Galdames J. , Navea D. , Farfán M. J. , Salas C. 
Published: June 2019

AbstractInvasive fungal infections (IFIs) are the most frequent cause of morbidity and mortality in immunocompromised children. Voriconazole is the first-line antifungal choice in the treatment of IFIs like aspergillosis. Voriconazole pharmacokinetics vary widely among patients and voriconazole is metabolized mainly in the liver by the CYP2C19 enzyme, which is highly polymorphic. The CYP2C19*17 allele is characterized by the presence of four single nucleotide polymorphisms expressing an ultra-rapid enzyme phenotype with an accelerated voriconazole metabolism, is associated with low (sub-therapeutic) plasma levels in patients treated with the standard dose. Considering that in our center a high percentage of children have sub-therapeutic levels of voriconazole when treated with standard doses, we sought to determine the frequency of the CYP2C19*17 polymorphism (rs12248560) in a Chilean population and determine the association between voriconazole concentrations and the rs12248560 variant in immunocompromised children. First, we evaluated the frequency of the rs12248560 variant in a group of 232 healthy Chilean children, and we found that 180 children (77.6%) were non-carriers of the rs12248560 variant, 49 children (21.1%) were heterozygous carriers for rs12248560 variant and only 3 children (1.3%) were homozygous carriers for rs12248560 variant, obtaining an allelic frequency of 12% for variant in a Chilean population. To determine the association between voriconazole concentrations and the rs12248560 variant, we analyzed voriconazole plasma concentrations in a second group of 33 children treated with voriconazole. In these patients, carriers of the rs12248560 variant presented significantly lower voriconazole plasma concentrations than non-carriers (p = 0,011). In this study, we show the presence of the rs12248560 variant in a Chilean population and its accelerating effect on the pharmacokinetics of voriconazole in pediatric patients. From these data, it would be advisable to consider the variant of the patient prior to calculating the dosage of voriconazole.
Xylella fastidiosa: climate suitability of European continent
Authors: Godefroid Martin , Cruaud Astrid , Streito Jean-Claude , Rasplus Jean-Yves , Rossi Jean-Pierre 
Published: June 2019

AbstractThe bacteriumXylella fastidiosa (Xf)is a plant endophyte native to the Americas that causes diseases in many crops of economic importance (grapevine,Citrus, Olive trees etc).Xfhas been recently detected in several regions outside of its native range including Europe where little is known about its potential geographical expansion. We collected data documenting the native and invaded ranges of theXfsubspeciesfastidiosa,paucaand multiplex and fitted bioclimatic species distribution models (SDMs) to assess the potential climate suitability of European continent for those pathogens. According to model predictions, the currently reported distribution ofXfin Europe is small compared to the large extent of climatically suitable areas. The regions at high risk encompass the Mediterranean coastal areas of Spain, Greece, Italy and France, the Atlantic coastal areas of France, Portugal and Spain as well as the southwestern regions of Spain and lowlands in southern Italy. The extent of predicted climatically suitable conditions for the different subspecies are contrasted. The subspeciesmultiplex, and to a certain extent the subspeciesfastidiosa, represent a threat to most of Europe while the climatically suitable areas for the subspeciespaucaare mostly limited to the Mediterranean basin. These results provide crucial information for the design of a spatially informed European-scale integrated management strategy, including early detection surveys in plants and insect vectors and quarantine measures.
Effect of heritable symbionts on maternally-derived embryo transcripts
Authors: Mateos Mariana , Silva Nadisha O. , Ramirez Paulino , Higareda-Alvear Victor M. , Aramayo Rodolfo , Erickson James W. 
Published: June 2019

AbstractMaternally-transmitted endosymbiotic bacteria are ubiquitous in insects. Among other influential phenotypes, many heritable symbionts of arthropods are notorious for manipulating host reproduction through one of four reproductive syndromes, which are generally exerted during early developmental stages of the host: male feminization; parthenogenesis induction; male killing; and cytoplasmic incompatibility (CI). Major advances have been achieved in understanding mechanisms and identifying symbiont factors involved in reproductive manipulation, particularly male killing and cytoplasmic incompatibility. Nonetheless, whether cytoplasmically-transmitted bacteria influence the maternally-loaded components of the egg or early embryo has not been examined. In the present study, we investigated whether heritable endosymbionts that cause different reproductive phenotypes in Drosophila melanogaster influence the mRNA transcriptome of early embryos. We used mRNA-seq to evaluate differential expression in Drosophila embryos lacking endosymbionts (control) to those harbouring the male-killing Spiroplasma poulsonii strain MSRO-Br, the CI-inducing Wolbachia strain wMel, or Spiroplasma poulsonii strain Hyd1; a strain that lacks a reproductive phenotype and is naturally associated with Drosophila hydei. We found no consistent evidence of influence of symbiont on mRNA composition of early embryos, suggesting that the reproductive manipulation mechanism does not involve alteration of maternally-loaded transcripts. In addition, we capitalized on several available mRNA-seq datasets derived from Spiroplasma-infected Drosophila melanogaster embryos, to search for signals of depurination of rRNA, consistent with the activity of Ribosome Inactivating Proteins (RIPs) encoded by Spiroplasma poulsonii. We found small but statistically significant signals of depurination of Drosophila rRNA in the Spiroplasma treatments (both strains), but not in the symbiont-free control or Wolbachia treatment, consistent with the action of RIPs. The depurination signal was slightly stronger in the treatment with the male-killing strain. This result supports a recent report that RIP-induced damage contributes to male embryo death.
The Ratios of monounsaturated to saturated phosphatidylcholines in lung adenocarcinoma microenvironment analyzed by Liquid Chromatography-Mass spectrometry and imaging Mass spectrometry
Authors: Muranishi Yusuke , Sato Toshihiko , Ito Shinji , Satoh Junko , Yoshizawa Akihiko , Tamari Shigeyuki , Ueda Yuichiro , Yutaka Yojiro , Menju Toshi , Nakamura Tatsuo , Date Hiroshi 
Published: June 2019

AbstractAdenocarcinoma is the most common type of lung cancer, and can be classified into various histologic subtypes. However, little is known about the subtype-dependent variations in lipid metabolism processes. We performed dual lipidomic analyses using liquid chromatography–mass spectrometry (LC-MS) and matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) to identify possible biomarkers to distinguish adenocarcinoma specimens from normal lung specimens, and to determine if there are any differences in lipid metabolism among the histologic subtypes (lepidic, acinar, papillary, micropapillary, solid, and mucinous). LC-MS was used to characterize the lipid profiles of lung adenocarcinoma and normal lung tissue, and MALDI-IMS analysis was performed to confirm the results with information on lipid localization within the lung. LC-MS analysis found significant differences in the relative abundances of phosphatidylcholine (PC)(16:0/16:0) (P = 0.0432) and sphingomyelin (SM)(42:2) (P < 0.0001) between adenocarcinoma and normal lung specimens. The ratios of PC(16:0/16:1)/PC(16:0/16:0), PC(16:0/18:1)/PC(16:0/16:0), and PC(16:0/18:1)/PC(16:0/18:0) were significantly higher in adenocarcinoma specimens (P = 0.02221, P = 0.0004, and P = 0.0215, respectively). MALDI-IMS analysis confirmed that these ratios were significantly higher in adenocarcinoma regions of the lung. The ratio of PC(16:0–18:1)/PC(16:0–18:0) was significantly lower in solid subtypes than in other subtypes (P = 0.0028). The monounsaturated/saturated PC ratios may have applications in adenocarcinoma diagnoses and subtyping.
Optimized electrochemical performance of Ni rich LiNi0.91Co0.06Mn0.03O2 cathodes for high-energy lithium ion batteries
Authors: Lee Seung-Hwan , Lee Seul , Jin Bong-Soo , Kim Hyun-Soo 
Published: June 2019

AbstractWe report high electrochemical performances of LiNi0.91Co0.06Mn0.03O2 cathode material for high-energy lithium ion batteries. LiNi0.91Co0.06Mn0.03O2 is synthesized at various sintering temperatures (640~740 °C). The sintering temperatures affect crystallinity and structural stability, which play an important role in electrochemical performances of LiNi0.91Co0.06Mn0.03O2. The electrochemical performances are improved with increasing sintering temperature up to an optimal sintering temperature. The LiNi0.91Co0.06Mn0.03O2 sintered at 660 °C shows remarkably excellent performances such as initial discharge capacity of 211.5 mAh/g at 0.1 C, cyclability of 85.3% after 70 cycles at 0.5 C and rate capability of 90.6% at 2 C as compared to 0.5 C. These results validate that LiNi0.91Co0.06Mn0.03O2 sintered at 660 °C can be regarded as a next generation cathode.
Coupled flow accumulation and atmospheric blocking govern flood duration
Authors: Najibi Nasser , Devineni Naresh , Lu Mengqian , Perdigão Rui A. P. 
Published: June 2019

AbstractWe present a physically based Bayesian network model for inference and prediction of flood duration that allows for a deeper understanding of the nexus of antecedent flow regime, atmospheric blocking, and moisture transport/release mechanisms. Distinct scaling factors at the land surface and regional atmospheric levels are unraveled using this Bayesian network model. Land surface scaling explains the variability in flood duration as a function of cumulative exceedance index, a new measure that represents the evolution of the flood in the basin. Dynamic atmospheric scaling explains the cumulative exceedance index using the interaction between atmospheric blocking system and the synergistic model of wind divergence and atmospheric water vapor. Our findings underline that the synergy between a large persistent low-pressure blocking system and a higher rate of divergent wind often triggers a long-duration flood, even in the presence of moderate moisture supply in the atmosphere. This condition in turn causes an extremely long-duration flood if the basin-wide cumulative flow prior to the flood event was already high. Thus, this new land-atmospheric interaction framework integrates regional flood duration scaling and dynamic atmospheric scaling to enable the coupling of ‘horizontal’ (for example, streamflow accumulation inside the basin) and ‘vertical’ flow of information (for example, interrelated land and ocean-atmosphere interactions), providing an improved understanding of the critical forcing of regional hydroclimatic systems. This Bayesian model approach is applied to the Missouri River Basin, which has the largest system of reservoirs in the United States. Our predictive model can aid in decision support systems for the protection of national infrastructure against long-duration flood events.
Calaxin is required for cilia-driven determination of vertebrate laterality
Authors: Sasaki Keita , Shiba Kogiku , Nakamura Akihiro , Kawano Natsuko , Satouh Yuhkoh , Yamaguchi Hiroshi , Morikawa Motohiro , Shibata Daisuke , Yanase Ryuji , Jokura Kei , Nomura Mami , Miyado Mami , Takada Shuji , Ueno Hironori , Nonaka Shigenori , Baba Tadashi , Ikawa Masahito , Kikkawa Masahide , Miyado Kenji , Inaba Kazuo 
Published: June 2019

AbstractCalaxin is a Ca2+-binding dynein-associated protein that regulates flagellar and ciliary movement. In ascidians, calaxin plays essential roles in chemotaxis of sperm. However, nothing has been known for the function of calaxin in vertebrates. Here we show that the mice with a null mutation in Efcab1, which encodes calaxin, display typical phenotypes of primary ciliary dyskinesia, including hydrocephalus, situs inversus, and abnormal motility of trachea cilia and sperm flagella. Strikingly, both males and females are viable and fertile, indicating that calaxin is not essential for fertilization in mice. The 9 + 2 axonemal structures of epithelial multicilia and sperm flagella are normal, but the formation of 9 + 0 nodal cilia is significantly disrupted. Knockout of calaxin in zebrafish also causes situs inversus due to the irregular ciliary beating of Kupffer’s vesicle cilia, although the 9 + 2 axonemal structure appears to remain normal.
MCRIP1 promotes the expression of lung-surfactant proteins in mice by disrupting CtBP-mediated epigenetic gene silencing
Authors: Weng Jane S. , Nakamura Takanori , Moriizumi Hisashi , Takano Hiroshi , Yao Ryoji , Takekawa Mutsuhiro 
Published: June 2019

AbstractProper regulation of epigenetic states of chromatin is crucial to achieve tissue-specific gene expression during embryogenesis. The lung-specific gene products, surfactant proteins B (SP-B) and C (SP-C), are synthesized in alveolar epithelial cells and prevent alveolar collapse. Epigenetic regulation of these surfactant proteins, however, remains unknown. Here we report that MCRIP1, a regulator of the CtBP transcriptional co-repressor, promotes the expression of SP-B and SP-C by preventing CtBP-mediated epigenetic gene silencing. Homozygous deficiency of Mcrip1 in mice causes fatal respiratory distress due to abnormal transcriptional repression of these surfactant proteins. We found that MCRIP1 interferes with interactions of CtBP with the lung-enriched transcriptional repressors, Foxp1 and Foxp2, thereby preventing the recruitment of the CtBP co-repressor complex to the SP-B and SP-C promoters and maintaining them in an active chromatin state. Our findings reveal a molecular mechanism by which cells prevent inadvertent gene silencing to ensure tissue-specific gene expression during organogenesis.
Toxicity of combined exposure of ZnO nanoparticles (NPs) and myricetin to Caco-2 cells: changes of NP colloidal aspects, NP internalization and the apoptosis-endoplasmic reticulum stress pathway
Authors: Wu Chaohua , Luo Yunfeng , Liu Liangliang , Xie Yixi , Cao Yi 
Published: June 2019

Abstract
Phytochemicals as typical food components may significantly influence the toxicity of nanoparticles (NPs) in intestinal cells, indicating a need to evaluate the toxicological effects of NPs in a complex situation. Previous studies suggested that the anti-oxidative properties of phytochemicals were important to elicit cytoprotective effects against NP exposure. However, we recently found that the changes of signaling pathways may be more important for cytoprotective effects of phytochemicals. In this study, we investigated the influence of myricetin (MY) on the cytotoxicity of ZnO NPs in Caco-2 cells and the possible mechanism. MY at 50 μM showed minimal impact on the solubility and colloidal aspects of ZnO NPs, but protected Caco-2 cells from NP exposure as it increased the EC50 value. For comparison, dihydromyricetin (DMY; chemical analog of MY) increased the EC50 value to a much lesser extent. Exposure to ZnO NPs significantly induced intracellular Zn ions, whereas MY or DMY did not significantly influence the internalization of NPs. However, ZnO NPs significantly promoted the ratio of caspase-3/pro-caspase-3, which was inhibited by the presence of MY. Exposure to ZnO NPs did not significantly promote the biomarkers of endoplasmic reticulum (ER) stress, but co-exposure to ZnO NPs and MY significantly lowered the levels of a panel of ER stress biomarkers. In conclusion, these results suggested that MY could protect Caco-2 cells from ZnO NP exposure, which may not be related to the changes of colloidal stability or internalization of NPs but could be alternatively related to the reduction of ER stress leading to lower cleaved caspase-3.
Globular cluster candidates in the Galactic bulge: Gaia and VVV view of the latest discoveries
Authors: Gran F. , Zoccali M. , Contreras Ramos R. , Valenti E. , Rojas-Arriagada A. , Carballo-Bello J. A. , Alonso-García J. , Minniti D. , Rejkuba M. , Surot F. 
Published: June 2019

Context. Thanks to the recent wide-area photometric surveys, the number of star cluster candidates have risen exponentially in the last few years. Most detections, however, are based only on the presence of an overdensity of stars in a given region or an overdensity of variable stars, regardless of their distance. As candidates, their detection has not been dynamically confirmed. Therefore, it is currently unknown how many and which of the published candidates are true clusters and which are chance alignments.
Aims. We present a method to detect and confirm star clusters based on the spatial distribution, coherence in motion, and appearance on the color-magnitude diagram. We explain and apply this approach to one new star cluster and several candidate star clusters published in the literature.
Methods. The presented method is based on data from the second data release of Gaia complemented with data from the VISTA Variables in the Vía Láctea survey for the innermost bulge regions. This method consists of a nearest neighbors algorithm applied simultaneously over spatial coordinates, star color, and proper motions to detect groups of stars that are close in the sky, move coherently, and define narrow sequences in the color-magnitude diagram, such as a young main sequence or a red giant branch.
Results. When tested in the bulge area (−10 <  ℓ (deg) <  +10; −10 <  b (deg) <  +10) the method successfully recovered several known young and old star clusters. We report in this work the detection of one new, likely old star cluster, while deferring the others to a forthcoming paper. Additionally, the code has been applied to the position of 93 candidate star clusters published in the literature. As a result, only two of these clusters are confirmed as coherently moving groups of stars at their nominal positions.
Production of recombinant extracellular cholesterol esterase using consistently active promoters in Burkholderia stabilis
Authors: Yoshida Keitaro , Konishi Kenji , Magana-Mora Arturo , Rougny Adrien , Yasutake Yoshiaki , Muramatsu Shuji , Murata Satomi , Kumagai Toshitaka , Aburatani Sachiyo , Sakasegawa Shin-ichi , Tamura Tomohiro 
Published: June 2019

ABSTRACT
Burkholderia stabilis FERMP-21014 produces highly active cholesterol esterase in the presence of fatty acids. To develop an overexpression system for cholesterol esterase production, we carried out RNA sequencing analyses to screen strongly active promoters in FERMP-21014. Based on gene expression consistency analysis, we selected nine genes that were consistently expressed at high levels, following which we constructed expression vectors using their promoter sequences and achieved overproduction of extracellular cholesterol esterase under fatty acid-free conditions. Of the tested promoters, the promoter of BSFP_0720, which encodes the alkyl hydroperoxide reductase subunit AhpC, resulted in the highest cholesterol esterase activity (24.3 U mL−1). This activity level was 243-fold higher than that of the wild-type strain under fatty acid-free conditions. We confirmed that cholesterol esterase was secreted without excessive accumulation within the cells. The gene expression consistency analysis will be useful to screen promoters applicable to the overexpression of other industrially important enzymes.