bluuebunny/crossref_metadata_2025_split huggingface dataset:
A case of eccrine porocarcinoma characterized by a progressive increase in the level of Ki-67 index: case report and review of literature
Authors: Shen Jie , Pan Xinfa , Lu Yongfu , Pan Desheng , Ma Yuehui , Zhan Renya 
Published: October 2019

AbstractBackgroundEccrine porocarcinoma is an extremely rare skin adnexal malignant neoplasia with highly invasive and metastatic potential. We report an additional case of eccrine porocarcinoma with intracranial metastases. This case is characterized by a complete record of the progress of eccrine porocarcinoma, its immunohistochemistry after three operations showed a progressive increase in the level of Ki-67 index.Case presentationWe herein report a case of a 37-year-old-male with eccrine carcinoma occurring on the left posterior occipital scalp which invaded the skull and dura, presenting with progressive headache. This patient has performed three surgeries in total. During the last hospitalization, he underwent an extended surgical resection, lymphadenectomy, myocutaneous flap transplantation and vascular anastomosis in our institution. After surgery, he was treating with radiotherapy at 200 Gray in 12 fractions. But one year after the operation, he developed chest tightness, imaging examination and biopsy puncture revealed pulmonary metastasis.ConclusionIntracranial metastasis of eccrine porocarcinoma is a late event with poor prognosis. This case emphases on that progressively increased level of Ki-67 index may predict more chance to occur the intracranial metastasis of scalp eccrine porocarcinoma, long-term follow-up and appropriately dense follow-up interval is necessary.
Using a marine microalga as a chassis for polyethylene terephthalate (PET) degradation
Authors: Moog Daniel , Schmitt Johanna , Senger Jana , Zarzycki Jan , Rexer Karl-Heinz , Linne Uwe , Erb Tobias , Maier Uwe G. 
Published: October 2019

Abstract

Background
The biological degradation of plastics is a promising method to counter the increasing pollution of our planet with artificial polymers and to develop eco-friendly recycling strategies. Polyethylene terephthalate (PET) is a thermoplast industrially produced from fossil feedstocks since the 1940s, nowadays prevalently used in bottle packaging and textiles. Although established industrial processes for PET recycling exist, large amounts of PET still end up in the environment—a significant portion thereof in the world’s oceans. In 2016, Ideonella sakaiensis, a bacterium possessing the ability to degrade PET and use the degradation products as a sole carbon source for growth, was isolated. I. sakaiensis expresses a key enzyme responsible for the breakdown of PET into monomers: PETase. This hydrolase might possess huge potential for the development of biological PET degradation and recycling processes as well as bioremediation approaches of environmental plastic waste.

Results
Using the photosynthetic microalga Phaeodactylum tricornutum as a chassis we generated a microbial cell factory capable of producing and secreting an engineered version of PETase into the surrounding culture medium. Initial degradation experiments using culture supernatant at 30 °C showed that PETase possessed activity against PET and the copolymer polyethylene terephthalate glycol (PETG) with an approximately 80-fold higher turnover of low crystallinity PETG compared to bottle PET. Moreover, we show that diatom produced PETase was active against industrially shredded PET in a saltwater-based environment even at mesophilic temperatures (21 °C). The products resulting from the degradation of the PET substrate were mainly terephthalic acid (TPA) and mono(2-hydroxyethyl) terephthalic acid (MHET) estimated to be formed in the micromolar range under the selected reaction conditions.

Conclusion
We provide a promising and eco-friendly solution for biological decomposition of PET waste in a saltwater-based environment by using a eukaryotic microalga instead of a bacterium as a model system. Our results show that via synthetic biology the diatom P. tricornutum indeed could be converted into a valuable chassis for biological PET degradation. Overall, this proof of principle study demonstrates the potential of the diatom system for future biotechnological applications in biological PET degradation especially for bioremediation approaches of PET polluted seawater.
Fish consumption during menarche, menstruation, pregnancy and postpartum in Sikuani women from Meta, Colombia
Authors: Cubillos-Cuadrado Luisa Fernanda , Muñoz-Hernández Daniela Stephany , Vásquez-Londoño Carlos Alberto 
Published: October 2019

Abstract

Background
Societies have selected their food for health, cultural, religious, political, economical, and environmental reasons. Most of the food included in Sikuani traditional diet still comes from wild natural resources and involves numerous species of fish, mammals, birds, reptiles, amphibians, insects, and plants. During certain periods of the Sikuani women’s reproductive cycle, fish intake is avoided. The objective of this research is to study the conceptions underlying fish consumption regulations among Sikuani women at the Wacoyo Reservation, in Meta, Colombia.

Methods
We conducted a field study through interviews and participant observation with Sikuani Indigenous from the Wacoyo Reservation (Colombia). We inquired about the conceptions of fish consumption regulation by Sikuani women during the stages of the reproductive cycle. PCA (principal component analysis) was used to identify the most important characteristics of fish that are related to the avoidance of fish intake by Sikuani women during pregnancy. This study combines qualitative and quantitative analysis.

Results
It was found that during menarche and postpartum fish consumption is avoided by Sikuani women only before the ritual known as the prayer of the fish is performed. The menstruation does not imply significant regulations for fish intake, while during pregnancy there are multiple and specific avoidances for the consumption of fish. According to our results, there are some features of fish associated with their regulation on the diet of pregnant Sikuani women. The consumption of some fish is avoided during pregnancy because it is related to the appearance of disease caused by ainawi, protector spirits of aquatic animals.

Conclusions
The traditional diet of Sikuani women includes numerous fish species and an important proportion of them are avoided during menarche, menstruation, gestation, and postpartum. According to our results, there are some features of fish associated with their regulation on the diet of pregnant Sikuani women. The main reasons underlying the avoidance of fish consumption by Sikuani women are the prevention of human disease as well as the strengthening of communities and ecosystems resilience.
Effect of probiotic supplementation in nonalcoholic steatohepatitis patients: PROBILIVER TRIAL protocol
Authors: Silva-Sperb Amanda Souza , Moraes Helena Abadie , de Moura Bruna Concheski , Alves Bruna Cherubini , Bruch-Bertani Juliana Paula , Azevedo Vittoria Zambon , Dall’Alba Valesca 
Published: October 2019

Abstract

Background
Recently factors in the relationship between gut microbiota, obesity, diabetes and the metabolic syndrome have been suggested in the development and progression of nonalcoholic steatohepatitis (NASH). In this sense, this work aims to evaluate the effects of probiotic supplementation on intestinal microbiota modulation, degree of hepatic steatosis and fibrosis, inflammation, gut permeability, and body composition.

Methods
This double-blind, randomized clinical trial will include adult outpatients with a diagnosis of NASH confirmed by biopsy with or without transient elastography. All patients will undergo a complete anamnesis to investigate their alcohol consumption, previous history, medications, nutritional assessment (dietary intake and body composition), sarcopenia, physical activity level and physical and functional capacity, cardiovascular risk, biochemical parameters for assessment of inflammatory status, lipid profile, hepatic function, gut permeability, and assessment of microbiota. These procedures will be performed at baseline and repeated after 24 weeks (at the end of the study). Through the process of randomization, patients will be allocated to receive treatment A or treatment B. Both patients and researchers involved will be blinded (double-blind study). The intervention consists of treatment with a probiotic mix (Lactobacillus acidophillus + Bifidobacterium lactis + Lactobacillus rhamnosus + Lactobacillus paracasei, 1 x 109 CFU for each) and the placebo which is identical in all its characteristics and packaging. Patients will be instructed to consume two sachets/day during 24 weeks and to report any symptoms or side effects related to the use of the sachets. Adherence control will be carried out through the patient’s notes on a form provided, and also by checking the number of sachets used.

Discussion
The final results of study will be analyzed and disseminated in 2020.

Trial registration
ClinicalTrials.gov, ID: NCT03467282. Registered on 15 March 2018.
The Transposon Registry
Authors: Tansirichaiya Supathep , Rahman Md. Ajijur , Roberts Adam P. 
Published: October 2019

Abstract
Transposable elements in prokaryotes are found in many forms and therefore a robust nomenclature system is needed in order to allow researchers to describe and search for them in publications and databases. Here we provide an update on The Transposon Registry which allocates numbers to any prokaryotic transposable element. Additionally, we present the completion of registry records for all transposons assigned Tn numbers from Tn1 onwards where sequence data or publications exist.
Knowledge of neonatal danger signs and associated factors among mothers who gave birth during the last 4 months while attending immunization services in Harar town public health facilities, Ethiopia, 2017
Authors: Welay Fissaha Tekulu , Kassa Nega Assefa , Gebremeskel Gebrekiros Aregawi , Assefa Natnael Etsay , Mengesha Meresa Berwo , Weldemariam Mulu Gebretsadik , Gebremeskel Senait Gebreslasie 
Published: October 2019

Abstract

Objective
The purpose of this study was to assess knowledge about neonatal danger signs and associated factors among mothers who gave birth in the last 4 months attending immunization services.

Result
The study recruited 432 mothers to participate. A knowledge score of neonatal danger signs was found [32.9% (95% CI 28.9%, 37%)]. Mothers educated to secondary level were 4.9 times more likely to know about neonatal danger signs [(AOR = 4.9, 95% CI (1.15, 21). Similarly, mothers whose husband educated to college and above [AOR = 4.95, 95% CI (1.15, 21)], and being multipara mother [(AOR = 2.59, 95% CI (1.05, 6.6)], were factors significantly associated with good knowledge of mothers about neonatal danger signs.
Recent advances in delivery of veterinary DNA vaccines against avian pathogens
Authors: Jazayeri Seyed Davoud , Poh Chit Laa 
Published: October 2019

AbstractVeterinary vaccines need to have desired characteristics, such as being effective, inexpensive, easy to administer, suitable for mass vaccination and stable under field conditions. DNA vaccines have been proposed as potential solutions for poultry diseases since they are subunit vaccines with no risk of infection or reversion to virulence. DNA vaccines can be utilized for simultaneous immunizations against multiple pathogens and are relatively easy to design and inexpensive to manufacture and store. Administration of DNA vaccines has been shown to stimulate immune responses and provide protection from challenges in different animal models. Although DNA vaccines offer advantages, setbacks including the inability to induce strong immunity, and the fact that they are not currently applicable for mass vaccination impede the use of DNA vaccines in the poultry industry. The use of either biological or physical carriers has been proposed as a solution to overcome the current delivery limitations of DNA vaccines for veterinary applications. This review presents an overview of the recent development of carriers for delivery of veterinary DNA vaccines against avian pathogens.
The therapeutic effects of microRNAs in preclinical studies of acute kidney injury: a systematic review protocol
Authors: Zankar Sarah , Rodriguez Rosendo A. , Vinas Jose Luis , Burns Kevin D. 
Published: October 2019

Abstract

Background
Acute kidney injury (AKI) causes significant morbidity and mortality in humans, and there are currently no effective treatments to enhance renal recovery. MicroRNAs (miRNAs) are short chain nucleotides that regulate protein expression and have been implicated in the pathogenesis of AKI. Recently, preclinical studies in vivo have uncovered a therapeutic role for administration of specific miRNAs in AKI. However, the overall benefits of this strategy in preclinical studies have not been systematically reviewed, and the potential for translation to human studies is unclear.

Aim
The primary aim is to conduct a systematic review of the therapeutic properties of miRNAs in preclinical studies of AKI. The secondary aim is to determine potential adverse effects of miRNA administration in these studies.

Methods
A comprehensive search strategy will identify relevant studies in AKI in vivo models, using the MEDLINE, EMBASE, OVID, PUBMED, and Web of Science databases. The search strategy will include terms for mammalian (non-human) AKI models, including injury related to ischemia/reperfusion, nephrotoxicity, sepsis, contrast agents, cardio-pulmonary bypass, and hemorrhagic shock. Interventions will be defined as direct administration of exogenous miRNAs or antagonists of miRNAs, as well as maneuvers that alter expression of miRNAs that are mechanistically linked to AKI outcomes. The primary outcomes will be indices of kidney function and structure, and there will be no restriction on comparator interventions. Two independent investigators will initially screen abstracts, and selected articles that meet eligibility criteria will be reviewed for data abstraction and analysis. The SYRCLE RoB tool for animal studies will determine risk of bias, and meta-analysis will be performed as appropriate. The GRADE methodology will assess the quality of evidence.

Discussion
The administration of selective miRNA mimics or antagonists exerts beneficial effects in mammalian models of AKI, although multiple obstacles must be addressed prior to translation to human clinical trials. The proposed systematic review will document key miRNA candidates, and determine effect size estimates and sources of outcome bias. The review will also identify gaps in knowledge and guide future directions in AKI research.

Systematic review registration
PROSPERO CRD42019128854
Neurocognitive outcome of school-aged children with congenital heart disease who underwent cardiopulmonary bypass surgery: a systematic review protocol
Authors: Feldmann Maria , Ullrich Cinzia , Bataillard Célina , Knirsch Walter , Gosteli-Peter Martina A. , Latal Beatrice , Held Ulrike 
Published: October 2019

AbstractBackgroundOver the past decades, survival rates of children born with congenital heart disease (CHD) have increased dramatically. Progress in prenatal diagnosis, less-invasive catheter techniques and perioperative intensive care as well as surgical techniques have led to an increased focus on extracardiac comorbidities, including potential neurodevelopmental sequelae associated with CHD. A growing body of literature reports impairments in early and school-age developmental outcome; however, there is a substantial variability in the spectrum of examined CHD types, assessment ages and applied test batteries. Furthermore, little information is available on executive function impairments in this population. Therefore, the aim of this systematic review is to determine the impact of CHD on intellectual outcome and executive functioning at school age and to determine risk factors for impaired outcomes by means of a systematic search.MethodsA systematic review of literature that reports neurodevelopmental outcome in children with CHD undergoing cardiopulmonary bypass surgery. Intelligence quotient or executive function scores will be considered primary outcomes. Databases such as Cochrane, EMBASE, MEDLINE and PsycINFO will be searched.DiscussionThe results of this systematic review will summarize the current evidence on intellectual and executive function outcome after cardiopulmonary bypass surgery in school-age children with CHD. This review will thus be the basis for better patient and parental counselling and the establishment of tailored follow-up programmes and interventional trials.Systematic review registrationIn accordance with the guidelines, our systematic review protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO) on January 9, 2019 (CRD42018086568). PROSPEROCRD42019118736.
The existence of sign-changing solution for a class of quasilinear Schrödinger–Poisson systems via perturbation method
Authors: Chen Lizhen , Feng Xiaojing , Hao Xinan 
Published: October 2019

Abstract
This paper is concerned with the existence of a sign-changing solution to a class of quasilinear Schrödinger–Poisson systems. There are some technical difficulties in applying variational methods directly to the problem because the quasilinear term makes it impossible to find a suitable space in which the corresponding functional possesses both smoothness and compactness properties. In order to overcome the difficulties caused by nonlocal term and quasi-linear term, we shall apply the perturbation method by adding a 4-Laplacian operator to consider the perturbation problem. And then, by using the approximation technique, a sign-changing solution with precisely two nodal domains is derived.
Some new inequalities for generalized fractional conformable integral operators
Authors: Nisar Kottakkaran Sooppy , Rahman Gauhar , Khan Aftab 
Published: October 2019

Abstract
The present paper aims to establish certain new classes of integral inequalities for a class of n ($n\in \mathbb{N}$
n
∈
N
) positive continuous and decreasing functions by utilizing the generalized fractional conformable integral operators (FCIO) recently defined by Khan and Khan. From these results, we also derive several particular cases.
‘Remark or retake’? A study of candidate performance in IELTS and perceptions towards test failure
Authors: Pearson William S. 
Published: October 2019

Abstract
It is becoming increasingly important for individuals for whom English is a second language to demonstrate their linguistic credentials for academic, work and employment purposes. One option is to undertake International English Language Testing System (IELTS), which involves attempting to meet the linguistic entrance criteria set by a gatekeeping institution in the skills of listening, reading, writing, and speaking. Yet limited information is available in the public domain concerning the success of test-takers in meeting cut-off criteria set by the 10,000 or so organisations that utilise IELTS. The present study analyses the relationship between the test results and stated band score objectives of a cohort of 600 IELTS candidates, who shared their results on a social networking platform. It was uncovered that more test-takers failed to meet their band score goals (n = 281) than achieved them (n = 245), with many requiring high level linguistic goals to maximise their prospects in immigration systems. Thematic analysis was employed to explore the seldom-heard perspectives of the test-takers who missed their targets, and thereby ‘failed’ the IELTS test. Far more candidates held perspectives that constituted a rejection of their overall or sub-test score in comparison with those who were accepting of their results. Candidates’ incredulity was notably acute concerning the accuracy of Speaking and Writing assessment, likely fuelled by a mistrust in single examiner marking and a lack of detailed test performance feedback to explain what went wrong.
Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
Authors: Palacios-Moreno Juan , Rubio Cecilia , Quilhot Wanda , Cavieres M. Fernanda , de la Peña Eduardo , Quiñones Natalia V. , Díaz Hugo , Carrión Flavio , Henríquez-Roldán Carlos F. , Weinstein-Oppenheimer Caroline R. 
Published: October 2019

Abstract

Background
Epanorin (EP) is a secondary metabolite of the Acarospora lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that EP inhibits cancer cell growth. MCF-7 breast cancer cells, normal fibroblasts, and the non-transformed HEK-293 cell line were exposed to increasing concentrations of EP, and proliferation was assessed by the sulforhodamine-B assay.

Results
MCF-7 cells exposed to EP were examined for cell cycle progression using flow cytometry, and DNA fragmentation was examined using the TUNEL assay. In addition, EP’s mutagenic activity was assessed using the Salmonella typhimurium reverse mutation assay. The data showed that EP inhibits proliferation of MCF-7 cells, and it induces cell cycle arrest in G0/G1 through a DNA fragmentation-independent mechanism. Furthermore, EP’s lack of overt cytotoxicity in the normal cell line HEK-293 and human fibroblasts in cell culture is supported by the absence of mutagenic activity of EP.

Conclusion
EP emerges as a suitable molecule for further studies as a potential antineoplastic agent.
Total joint reconstruction using computer-assisted surgery with stock prostheses for a patient with bilateral TMJ ankylosis
Authors: Rhee Seung-Hyun , Baek Seung-Hak , Park Sang-Hun , Kim Jong-Cheol , Jeong Chun-Gi , Choi Jin-Young 
Published: October 2019

Abstract

Backgrounds
The purpose of this study is to discuss the total joint reconstruction surgery for a patient with recurrent ankylosis in bilateral temporomandibular joints (TMJs) using three-dimensional (3D) virtual surgical planning, computer-aided manufacturing (CAD/CAM)-fabricated surgical guides, and stock TMJ prostheses.

Case presentation
A 66-year-old female patient, who had a history of multiple TMJ surgeries, complained of severe difficulty in eating and trismus. The 3D virtual surgery was performed with a virtual surgery software (FACEGIDE, MegaGen implant, Daegu, South Korea). After confirmation of the location of the upper margin for resection of the root of the zygoma and the lower margin for resection of the ankylosed condyle, and the position of the fossa and condyle components of stock TMJ prosthesis (Biomet, Jacksonville, FL, USA), the surgical guides were fabricated with CAD/CAM technology. Under general anesthesia, osteotomy and placement of the stock TMJ prosthesis (Biomet) were carried out according to the surgical planning. At 2 months after the operation, the patient was able to open her mouth up to 30 mm without complication.

Conclusion
For a patient who has recurrent ankylosis in bilateral TMJs, total joint reconstruction surgery using 3D virtual surgical planning, CAD/CAM-fabricated surgical guides, and stock TMJ prostheses may be an effective surgical treatment option.