bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protective effects of hydrogen gas in a rat model of branch retinal vein occlusion via decreasing VEGF-α expression
Authors: Long Pan , Yan Weiming , He Mengshan , Zhang Qianli , Wang Zhe , Li Manhong , Xue Junhui , Chen Tao , An Jing , Zhang Zuoming 
Published: March 2019

Abstract
Background: Oxidative stress (OS) is an essential factor in the pathogenesis of branch retinal vein occlusion (BRVO). Studies have demonstrated the role of hydrogen gas in the regulation of OS. This study was designed to evaluate the effecacy of hydrogen gas on the BRVO rat model.
Methods: Twenty-four BRVO rats were randomly divided into two groups: the hydrogen gas (H) group (42% H2, 21% O2, 37% N2) and the model (M) group (21% O2, 79% N2). Rats in the H group inhaled hydrogen gas for 8 h every day up to 30 d post-occlusion. Twelve age-matched healthy rats served as the control (C) group. Retinal function and morphology were detected at 1, 7, 14 and 30 d post-occlusion. Furthermore, the expression of vascular endothelial growth factor (VEGF-α) was detected by immunofluorescent staining.
Results: Full-field electroretinography (ffERG) revealed that the amplitude of the b-wave (dark-adaptation 3.0 response), the amplitude of the OPs2 wave and the light-adapted flicker response in the H group were all higher than those in the M group at 7 d post-occlusion (all p<0.05). The reopen time of occlusive retinal vessels in the H group was 2.235 ± 1.128 d, which was shorter than that in the M group (4.234±2.236 d, p<0.05). The rats in the H group had a thinner IPL+GCL+NFL and an increased total retina compared with those in the M group at 3 d post-occlusion (p<0.05), while the rats in the H group had a thicker INL, IPL+GCL+NFL and total retina compared with those at 7, 14 and 30 d post-occlusion (p<0.05). Moreover, the flow velocity of ear vein blood was increased in the H group compared with that in the M group (p<0.05). The expression of VEGF-α in the H group was dramatically decreased compared with that in the M group at 1, 7 and 14 d post-occlusion (p<0.05), while the expression kept in similar level at 30 d post-occlusion (p>0.05).
Conclusions: Our findings demonstrate that inhalation of hydrogen gas could alleviate retinal oedema, shorten reopen time and improve retinal function, and the potential mechanism might be related to a decrease in VEGF-α expression.
Changes in macular pigment optical density with age in healthy and in patients with dry age-related macular degeneration: a cross sectional study
Authors: Jung Woo Hyun , Lee Jae Hyup , Ban Ji Hoon , Park No Hae , Lee Jung Hoo , Kwak Hyun Duck , Kim Ki Soo , CHANG IN BOEM 
Published: March 2019

Abstract
Background

To study the changes in macular pigment optical density (MPOD) with age in healthy eyes and in patients with dry age-related macular degeneration (AMD) using the Macular Pigment Screener II (MPSII®).

Methods

One hundred and twenty-six eyes of 126 individuals (51 male, 75 female) were retrospectively evaluated. The MPOD was measured using the MPSII®, which uses a heterochromatic flicker photometry (HFP) method, and the estimated values were reviewed. Spearman’s correlation test was used to evaluate the correlations between the MPOD and age. Simple linear regression analysis was performed to obtain determine the association between the MPOD and age and MPOD. The Kruskal-Wallis test was used to compare the MPOD between among the four groups, and the Mann-Whitney U test was utilized to compare the MPOD between the dry AMD group and the elderly controls

Results

Estimated MPOD values decreased significantly with increasing age (Spearman’s correlation coefficient, -0.239; p=0.008) in the general population. In the simple regression analysis, a statistically significant linear regression model was observed, and the estimated values of the MPOD decreased by -0.005 as the age increased by one 1 year (p=0.001, Eestimated value of MPOD value = 0.884 – 0.005 ×x Aage). In the healthy population, the estimated MPOD values exhibited a decreasing trend with age, but there were no significant differences according to age, with the exception of patients with dry AMD (p=0.201). The MPOD was significantly lower in patients with dry AMD than in age-matched healthy controls (p=0.001).

Conclusions

Measurement of the MPOD using the MPSII® is feasible in both healthy individuals and in patients with dry AMD. Early detection of AMD and the contributing risk factors may be possible with regular MPOD screening in elderly patients or in patients with a reduced MPOD.
Unilateral Inferior Oblique Anterior Transposition for Asymmetric Dissociated Vertical Deviation with Unilateral Inferior Oblique Over-action
Authors: Wu Shuang-Qing , Xu Qi-Bin , Sheng Wen-Yan , Zhu Li-Wei 
Published: March 2019

Abstract
Background: To evaluate the results of unilateral inferior oblique anterior transposition (IOAT) for dissociated vertical deviation (DVD) combined with inferior oblique over-action (IOOA).
Methods：Retrospective chart review of the records of all patients with asymmetric DVD combined with unilateral IOOA in the non-dominant eye, who received unilateral IOAT on the non-dominant eye. No other muscles were operated simultaneously. The amount of DVD and IOOA before and after the operation was measured and statistically analyzed.
Results: Seventeen patients were included. Mean age at surgery was 23.5 ± 8.4 years (range 12 - 38). The mean postoperative follow-up period was 15.7 ± 7.2 months (range 6 - 32). Primary position DVD was 19.6 ± 5.4 PD (range 14 - 36) preoperatively, which decreased significantly to 2.9 ± 2.0 PD (range 0 - 8) postoperatively (P < 0.01). There were 2, 7, and 8 patients with +1, +2, and +3 IOOA preoperatively, respectively, which was reduced significantly to 0.3 ± 0.4 postoperatively.
Conclusions: Unilateral IOAT was recommended in patients with asymmetric DVD coexists with unilateral IOOA.
Agreement between anterior segment Optical Coherence Tomography, Lenstar and Ultrasound Biomicroscopy measurements of anterior segment parameters
Authors: Yu Ziyan , Huang Ting , Yu Qiao , Kong Jun , Qu Bo 
Published: March 2019

Abstract
Purpose

To compare anterior chamber depth (ACD) and central corneal thickness (CCT) measurements by anterior segment optical coherence tomography (AS-OCT), Lenstar and ultrasound biomicroscopy (UBM).

Methods

A retrospective cross section study. A total of 83 eyes from 54 patients were included. Central corneal thickness (CCT) and anterior chamber depth (ACD) values obtained from three devices (AS-OCT, Lenstar and UBM) were recorded. All patients were collected from Oct. 2015 to Aug. 2016 in the Fourth Affiliated Hospital of China Medical University. Differences of these three devices (ACD and CCT) were statistically analyzed by randomized blocks analysis. Pearson correlative analysis and Bland-Altman analysis were used to compare the correlation and agreement.

Results

There’s no significant difference in measuring ACD by Lenstar and UBM (P>0.05), but there were significant differences in other two groups (AS-OCT and UBM, AS-OCT and Lenstar) (P<0.05). There were significant differences in the mean CCT measured by the three devices (P<0.05). There were high correlation in measuring ACD (all P<0.001, r1=0.928,r2=0.982,r3=0.932, respectively), and CCT(all P<0.001,r1=0.957,r2=0.949,r3=0.928, respectively).

Conclusion

AS-OCT, Lenstar and UBM yielded comparable results and are all can be used interchangeably for anterior segment measurements.
Synthesis and Antibacterial Activity of Mefloquine-Based Analogs Against Sensitive and Resistant Mycobacterium tuberculosis Strains
Authors: da Silva Araújo Adriele , Moraes Adriana M. , Lourenço Maria C.S. , Pessoa Cláudia O. , da Silva Emerson T. , de Souza Marcus V.N. 
Published: March 2019

Background and Introduction:Mefloquine, a drug used to prevent and treat malaria is described possessing activity against the Mycobacterium tuberculosis (Mtb) as well as against multidrugresistant tuberculosis (MDR) and other types of bacteria. Despite their importance, few compounds based on the Mefloquine nucleus have been synthesized and evaluated against TB.Materials and Methods:For the synthesis of all the compounds based on the Mefloquine nucleus we used a synthetic route which utilized the key derivative 4-methoxy-2,8-bis(trifluoromethyl)quinoline 2 as starting material. The compounds 3 (a-c), 4 (a-b) were synthesized after one step by reaction of 2 with appropriate amines substituted. The chloro derivatives 5 and 6 were obtained from compounds 4b and 4a by treatment with SOCl2 in CH2Cl2 at reflux in 75 and 80% yield, respectively. The analogue 6 was converted to 7 after treatment with ethanolamine under heating at 90oC in 64% yield and to the azido derivative 8 in 56% after reaction with sodium azide in MeOH at reflux for 2 h. The analogue 9 was obtained after reaction of 5 with ethanolamine at 90oC for 1 h in 90% yield. All the new compounds were identified by detailed spectral data, including 1H NMR, 13C NMR and high resolution mass spectra. All the compound were evaluated for their in vitro antibacterial activity against sensitive Mycobacterium tuberculosis ATCC 27294, using the microplate Alamar Blue assay (MABA). The more active compounds 3c, 7, and 9 were also evaluated against resistant strain SR 2571/0215 (resistant to Rifampicin and Isoniazid) by above method. All compounds were tested against three cancer cell lines: SF-295 (glioblastoma), HCT-116 (colon) and PC-3 (prostate) using the MTT assay.Results:All the planned ten compounds were synthetically obtained in good global yield, displaying activity against sensitive Mycobacterium tuberculosis in vitro, with exception of one, with MIC values between 37.2 and 154.8 µM. The compounds 3c (37.2 µM), 7 (68.1 µM) and 9 (65.6 µM) showed the highest activity in this series with MIC values similar when compare to the standard Mefloquine (30 – 60 µM), being 3c the most potent. The more active compounds 3c, 7, and 9 were also evaluated against resistant strain, displaying MIC of 37.2, 136.2 and 65.6 µM, respectively. All compounds were tested against three cancer cell lines and showed low cytotoxicity.Conclusion:All synthesized compounds, with the exception of 5, exhibited activity against the Mtb. Compound 3c was the most potent against resistant and sensitive Mtb in this series, with MIC value of 37.2 µM. All compounds showed low cytotoxicity. These findings could be considered a good model to develop possible lead compounds in the fight against TB based on Mefloquine nucleus.
On-Pump Coronary Artery Bypass Graft: The State of the Art
Authors: Cuminetti Giovanni , Bonadei Ivano , Vizzardi Enrico , Sciatti Edoardo , Lorusso Roberto 
Published: March 2019

Background:Coronary artery bypass grafting (CABG) remains the standard of care for patients with coronary artery disease (CAD). Debate exists concerning several factors, which include percutaneous coronary intervention (PCI) vs. CABG, single vs. bilateral mammary artery grafts, radial artery vs. saphenous vein grafts, right internal mammary artery vs. radial artery grafts, endoscopic vs. open vein-graft harvesting, and on-pump vs. off- pump surgery.:Moreover, challenging is the management of diabetic patients with CAD undergoing CABG. This review reports current indications, practice patterns, and outcomes of CABG.Methods:Randomized controlled trials comparing CABG to other therapeutical strategies for CAD were searched through MEDLINE, EMBASE, Cochrane databases, and proceedings of international meetings.Results:Large multicenter randomized and observational studies (SYNTAX, BEST, PRECOMBAT, ASCERT) have reported excellent outcomes in CABG patients, with always fewer rates of operative mortality and major morbidity, than PCI. The 10-year follow-up of ARTS II trial showed no difference between single and bilateral mammary artery. BARI 2D, MASS II, CARDia, FREEDOM trials showed that CABG is the best choice for diabetic patients.Conclusion:CABG still represents one of the most widespread major surgeries, with well-known benefits on symptoms and prognosis in patients with CAD. However, further studies and follow-up data are needed to validate these evidences.
Adaptive Elman Model of Gene Regulation Network Based on Time Series Data
Authors: Cao Shengxian , Wang Yu , Tang Zhenhao 
Published: March 2019

Background:Time series expression data of genes contain relations among different genes, which are difficult to model precisely. Slime-forming bacteria is one of the three major harmful bacteria types in industrial circulating cooling water systems.Objective:This study aimed at constructing gene regulation network(GRN) for slime-forming bacteria to understand the microbial fouling mechanism.Methods:For this purpose, an Adaptive Elman Neural Network (AENN) to reveal the relationships among genes using gene expression time series is proposed. The parameters of Elman neural network were optimized adaptively by a Genetic Algorithm (GA). And a Pearson correlation analysis is applied to discover the relationships among genes. In addition, the gene expression data of slime-forming bacteria by transcriptome gene sequencing was presented.Results:To evaluate our proposed method, we compared several alternative data-driven approaches, including a Neural Fuzzy Recurrent Network (NFRN), a basic Elman Neural Network (ENN), and an ensemble network. The experimental results of simulated and real datasets demonstrate that the proposed approach has a promising performance for modeling Gene Regulation Networks (GRNs). We also applied the proposed method for the GRN construction of slime-forming bacteria and at last a GRN for 6 genes was constructed.Conclusion:The proposed GRN construction method can effectively extract the regulations among genes. This is also the first report to construct the GRN for slime-forming bacteria.
Inhibition of Polo-Like Kinase 1 by BI2536 Reverses the Multidrug Resistance of Human Hepatoma Cells In Vitro and In Vivo
Authors: Li Hui Y. , Luo Fei , Li Xiao Y. , Fu Xi F. , He Jie F. , Tian Yan Z. , Zhu Jun J. , Chu Xin Y. , Zhao Hao L. 
Published: March 2019

Background:
Multi Drug Resistance (MDR) is the phenomenon that cancers develop resistance to
majority of chemotherapy drugs and is a serious obstacle to the treatment for Hepatocellular Carcinoma (HCC).
Polo-Like Kinase 1 (PLK1) is a serine/threonine kinase associated with tumor growth and clinical prognosis in
HCC and BI2536 is its potent inhibitor with IC50 of 0.83nM.

Aims:
To test whether the down-regulation of PLK1 by its inhibitor BI2536 would have beneficial effects on the
reversal of MDR in HCC cells.

Methods:
The CCK-8 assay was used to determine the viability of HepG2/ADM and SMMC7721/ADM cells
and their parental cells treated with BI2536. Then animal model studies were performed. Cell invasion assay and
wound healing assay were used to determine the invasion ability and motility. Flow cytometric was used to test
the apoptosis induced by BI2536. Western blot and quantitative real-time PCR were performed to test the
change of expression of MDR and apoptosis-related gene.

Results:
BI2536 down-regulated the expression of PLK1 protein and mRNA specifically. BI2536 can significantly
reduce IC50 for ADM and other drugs in ADM-resistant HCC cells. Meanwhile, it inhibited cell viability,
proliferation, and invasion, and induced cell cycle arrest and apoptosis in HCC cells with MDR.

Conclusion:
Our results suggest that PLK1 inhibitor BI2536 can re-sensitize HCC cancer cell with MDR
through induction of apoptosis. Thus, PLK1 inhibitor BI2536 may act as an effective chemotherapeutic drug in
the clinical treatment of HCC patients with MDR.
Vietnamese Young Graduate Employees’ Evaluation of Sources of Self-efficacy at Work
Authors: Bui Thi Hong Thai , Nguyen Thi Anh Thu , Nguyen Thi Mui 
Published: March 2019

Background:Self-efficacy at work is considered to be one of the factors affecting employees’ quality of work life and professional development. Understanding the sources of employees’ self-efficacy at work is essential, especially in the context of Viet Nam’s Doi Moi (Renovation) policy that has led to wide-reaching socio-economic changes in Viet Nam, including the workplace over the past 30 years.Methods:This article is based on a quantitative study of 166 Vietnamese graduates with less than 2-year working experience at their organizations, followed by 11 interviews. The study seeks to demonstrate their evaluation of the sources of self-efficacy at work.Findings:The results show that “physiological and emotional states”, “vicarious experiences” and “verbal persuasion” have a significantly positive correlation with self-efficacy at work while no remarkable association is observed for “mastery experiences”. In addition, the content of the interviews further clarifies the above-mentioned results.Conclusion:The findings suggest the important role of cultural-social factors and the characteristics of the workforce in Viet Nam in interpreting employee’s self-efficacy.
Convergence of Canonical and Non-Canonical Wnt Signal: Differential Kat3 Coactivator Usage
Authors: Lai Keane K.Y. , Nguyen Cu , Lee Kyung-Soon , Lee Albert , Lin David P. , Teo Jia-Ling , Kahn Michael 
Published: March 2019

Background:The ancient and highly evolutionarily conserved Wnt signaling pathway is critical in nearly all tissues and organs for an organism to develop normally from embryo through adult. Wnt signaling is generally parsed into “canonical” or Wnt-β-catenin-dependent or “non-canonical” β-catenin-independent signaling. Even though designating Wnt signaling as either canonical or noncanonical allows for easier conceptual discourse about this signaling pathway, in fact canonical and non-canonical Wnt crosstalk regulates complex nonlinear networks.Objective:In this perspective, we discuss the integration of canonical and non-canonical Wnt signaling via differential Kat3 (CBP and p300) coactivator usage, thereby regulating and coordinating gene expression programs associated with both proliferation and cellular differentiation and morphogenesis.Methods:Pharmacologic inhibitors, cell culture, real-time PCR, chromatin immunoprecipitation, protein immunoprecipitation, Western blotting, reporter-luciferase, protein purification, site-directed mutagenesis, in vitro phosphorylation and binding assays, and immunofluorescence were utilized.Conclusion:Coordinated integration between both canonical and non-canonical Wnt pathways appears to be crucial not only in the control of fundamental morphologic processes but also in the regulation of normal as well as pathologic events. Such integration between both canonical and non-canonical Wnt signaling is presumably effected via reversible phosphorylation mechanism (e.g., protein kinase C) to regulate differential β -catenin/Kat3 coactivator usage in order to coordinate proliferation with differentiation and adhesion.
Knowledge, Attitude and Practices Slaughterhouse Management in Special Capital Region of Jakarta
Authors: Sidablok Hasudungan Agustinus , - Macfud , - Nahrowi , Pandjaitan Nurmala K 
Published: March 2019

The objective of this research is understanding the correlation of characteristics with level of knowledge, attitude, and practice of slaughterhouse management concerning hygiene, sanitation, and waste management. Research objects in this study are 3 types of slaughterhouse in Jakarta; pig, poultry, and ruminant slaughterhouse. The aim of this study is determine the correlation of knowledge, attitude, and practice characteristics of slaughterhouse management related to hygiene, sanitation, and waste management. Tools used are questionnaire to assess knowledge, attitude, and practices of slaughterhouse management. Data collected consist by questionnaire filled by slaughterhouse agency. Data were analysed with SPSS® to calculate characteristic and variables correlation. On pig slaughterhouse management, there is correlation between educational background with knowledge (P<0.05; r=-0.804). On ruminant slaughterhouse, there is a correlation between income level with practice (P<0.05; r=-0.804) and knowledge level with attitude (P<0.05; r=0.641). On poultry slaughterhouse, there is a clear correlation between educational level with knowledge (P<0.05; r=0.686), income level with knowledge (P<0.06; r=-0.802), educational level with attitude (P<0.05; r=0.716), frequency of training with attitude (P<0.05; r=-0.741), frequency of training with practice (P<0.05; r=0.758), and employment status with practice (P<0.05; r=0.127). Training and socialization frequency must be improved especially for ruminant slaughterhouse for it is the location with the least number of training and socialization. Education level requirement for employment must also be increased since education level has a clear correlation with knowledge.
KAJIAN ERGONOMI LEMARI, MEJA DAN KURSI PROGRAM STUDI TEKNIK ARSITEKTUR (STUDI KASUS: RUANG STUDIO ARSITEKTUR UNIKA MUSI CHARITAS DI PALEMBANG)
Authors: Anggraeni Dhita Wahyu 
Published: March 2019

Abstract: Design architecture was the college the nucleus in engineering study program architecture. Activities exercise and finished the task is done in studios picture , that is the main facility required to smooth teaching and learning in lecture architecture .Instrumentality used in studios is furniture of a cupboard, tables and chairs .Its furniture must comply with needs that ergonomic .Study ergonomics to do with furniture in engineering study program architecture especially in classrooms , a studio and space duty the end .The purpose of this research is studying about the mebel-mebel have met the requirements ergonomics .The findings of the study give contribution to furniture next election .A measure in research that is athropometric .Methods used in this research is descriptive exploration with to match ergonomics furniture existing with users. From the review found that furniture is in studios is not adequate furniture ergonomic , who make a difference on the activities of done users.Keywords: furniture, ergonomics, activity usersAbstrak: Perancangan arsitektur merupakan mata kuliah inti dalam Program Studi Teknik Arsitektur. Kegiatan latihan dan menyelesaikan tugas dilakukan di dalam Ruang studio gambar, yang merupakan fasilitas utama yang dibutuhkan untuk kelancaran belajar mengajar pada perkuliahan Arsitektur. Sarana yang digunakan di dalam ruang studio adalah mebel berupa lemari, meja dan kursi. Mebel tersebut harus memenuhi standar kebutuhan yang ergonomis. Kajian ergonomi akan dilakukan terhadap mebel di Program Studi Teknik Arsitektur khususnya pada ruang kelas, studio dan ruang Tugas Akhir. Tujuan penelitian ini adalah mengkaji sejauhmana mebel-mebel tersebut telah memenuhi persyaratan ergonomi. Hasil kajian memberi konstribusi terhadap pemilihan mebel selanjutnya. Tolok ukur dalam penelitian yaitu athropometri. Metode yang digunakan dalam penelitian ini adalah deskriptif eksploratif dengan mencari hubungan antara ergonomi mebel yang sudah ada dengan para pengguna. Dari hasil kajian ditemukan bahwa mebel yang ada di ruang studio tidak memenuhi standar mebel yang ergonomis, yang memberi pengaruh terhadap aktivitas yang dilakukan pengguna.Kata kunci: mebel, ergonomi, aktivitas pengguna