bluuebunny/crossref_metadata_2025_split huggingface dataset:
The economics of fertility in the dairy herd
Authors: Stott A. W. , Veerkamp R. F. , Wassell T. R. 
Published: May 2019

AbstractA method to establish the economic optimum (minimum) cost of fertility in the dairy herd is described and demonstrated. A Markov chain model is used iteratively to establish the gross margin of the herd in the long term at various levels of oestrous detection rate and under two different rebreeding strategies. These gross margins are required by the optimization methodology. Under the initial assumptions reflecting current commercial practice in the United Kingdom, gross margin was £806 per cow. This figure varied by proportionately 0·15 over the range of oestrous detection rates assumed (0·4 to 0·7) while delaying rebreeding by 20 days caused gross margin to drop by approximately 0·04. It was concluded that it is important to optimize fertility control as well as rebreeding strategy in order to establish the economic impact of fertility in the dairy herd.The economic value of fertility was also expressed per unit of calving interval and adjusted calving interval (ACI). ACI was calculated by dividing calving interval by the proportion of cows not culled for reproductive failure. Under the assumptions made, the marginal value of calving interval at the optimum oestrous detection rate was £6·22 per day, rising to £7·44 per day if rebreeding was delayed. The corresponding figures for ACI were £1·57 per day and £1·24 per day. The range in marginal values at sub-optimal oestrous detection rates were £4·38 for calving interval and £0·61 for ACI. It was concluded that the lower variation in ACI at different levels of fertility may make it a more representative trait for inclusion in a selection index provided the necessary genetic parameters can be reliably estimated.
Growth equations for skeletal muscle derived from the cytonuclear ratio and growth constraining supplementary functions
Authors: Roux C. Z. 
Published: May 2019

AbstractFor purely hypertrophic muscle it is postulated that the growth rate in number of nuclei is proportional to the cytoplasmic mass per nucleus multiplied by a growth constraining supplementary function. Growth constraint depends on the distance from any one of the limit number of nuclei, the limit muscle mass or the limit cytoplasmic mass per nucleus. Furthermore, theory and evidence are presented for a power (allometric) relationship between total number of nuclei (n) and muscle mass (m) given by the equation n = gmh. Evidence points to two clusters of values for h, one in the vicinity of h = 2/3 and the other h = 1/2. Both may depend on a linear relationship between number of nuclei inside muscle fibre and fibre cross-sectional area. The difference between the two situations can be derived from basic assumptions on either local or systemic diffusion mediated control of the number or division of satellite cell nuclei, leading directly to values of h either equal to 2/3 or V2. For likely values of h and suitable choices of growth constraints, almost all well known growth functions in the literature are derived as potentially applicable to total number of nuclei, or muscle mass or their ratio. Muscle mass growth will show a sigmoidal form for h = 1. This explains sigmoidal growth in body mass as it is mostly dominated by muscle mass. A possible linear growth phase before maturity is explicable from the cessation of either length (h = 1) or nuclear (h = 0) growth in muscle fibres, while cytoplasmic growth continues to maturity. Furthermore, two rat examples indicate that whole body protein growth can be described by the equations derived for muscle mass growth.
Factors influencing the production of milk from pastoral cattle herds in Kenya
Authors: Roderick S. , Stevenson P. , Ndung’u J. 
Published: May 2019

AbstractThe Maasai of Kenya are typical of many pastoral communities in that they rely on milk as a major part of their diet. Milk production in three herds of Maasai cattle was studied from 1991 to 1996. Weekly measurements of milk extracted for human consumption were used to estimate lactation length, total offtake and mean daily production. Calf live-weight data were used to estimate total milk yield. Least-mean squares regression and analysis of variance tests were adopted to assess the effect of a number of variables on milk production parameters.Data from a total of 650 lactations and 383 calvings were collected. Mean daily offtake was 891 ml. The length of lactation of frequently milked cows was 372 (s.e. 7·96) days. Total lactation offtake was estimated at 305 I. Herd, parity level and year of calving differences were observed. Multiple regression analyses indicated that some of the variation in daily offtake could be explained by stage of lactation, season of milking, herd, parity and year of calving. An average total yield of 2·73 I/day was estimated for the first 90 days of lactation. Of this, approximately 0·4 was used for human consumption. Overall, seasonality was seen as the major variable influencing production.
Characterization ofin situfibre digestion of several fibrous foods
Authors: Escalona B. , Rocha R. , García J. , Carabaño R. , Blast C. de 
Published: May 2019

AbstractThe disappearance of neutral-detergent fibre (NDF) from six fibrous foods (lucerne hay, NaOH-treated barley straw, olive leaves, paprika meal, soya-bean hulls and sunflower hulls) when incubated in the rumen of sheep in nylon bags was investigated. Source of fibre, time of incubation and its interaction had a significant (P< 0·01) effect on NDF degradation. Stepwise regression analysis showed that the best single predictor for NDF degradation rate was the hemicellulose fraction of NDF (HEMndf). The variables selected to predict potential degradability and degradability of NDF at 72 h were the proportions of acid-detergent lignin in NDF (ADLndf, first step) and of acid-detergent cutin in ADL (ADCadl, second step). For NDF degradability at 12 h, the variables selected were the proportions of HEMndf, ADLndfand ADCadl, in the first, second and third step, respectively. The results showed that ADC is an important component to understand NDF degradability at both 12 and 72 h.
Can’t touch this
Authors: Clarke Amy , King Stuart , Leach Andrew , Van Acker Wouter 
Published: May 2019

Australia’s laid-back, sun-drenched beach lifestyle has been a celebrated and prominent part of its official popular culture for nigh on a century, and the images and motifs associated with this culture have become hallmarks of the country’s collective identity. Though these representations tend towards stereotype, for many Australians the idea of a summer holiday at the beach is one that is intensely personal and romanticised – its image is not at all urbanised. As Douglas Booth observed, for Australians the beach has become a ‘sanctuary at which to abandon cares – a place to let down one’s hair, remove one’s clothes […] a paradise where one could laze in peace, free from guilt, drifting between the hot sand and the warm sea, and seek romance’.1 Beach holidays became popular in the interwar years of the twentieth century, but the most intense burst of activity – both in touristic promotion and in the development of tourism infrastructure – accompanied the postwar economic boom, when family incomes were able to meet the cost of a car and, increasingly, a cheap block of land by the beach upon which a holiday home could be erected with thrift and haste. In subtropical southeast Queensland, the postwar beach holiday became the hallmark of the state’s burgeoning tourism industry; the state’s southeast coastline in particular benefiting from its warm climate and proximity to the capital, Brisbane. It was here – along the evocatively named Gold Coast (to Brisbane’s south) and Sunshine Coast (to its north) [1] – that many families experienced their first taste of what is now widely celebrated as the beach lifestyle [2]. As one reflection has it:
In the era before motels and resorts, a holiday at the Gold and Sunshine coasts usually meant either pitching a tent and camping by the beach or staying in a simple cottage owned by family or friends […] Simplicity, informality, individuality […] were the hallmarks of these humble places.2
Live Imaging and Analysis of Vasoactive Properties of Drugs Using an in-ovo Chicken Embryo Model: Replacing and Reducing Animal Testing
Authors: Swaminathan Akila , Balaguru Uma Maheswari , Manjunathan Reji , Bhuvaneswari Srinivasan , Kasiviswanathan Dharanibalan , Sirishakalyani Bandi , Nayak Prasunpriya , Chatterjee Suvro 
Published: May 2019

AbstractVasodilation occurs as a result of the relaxation of the smooth muscle cells present in the walls of blood vessels. Various suitable models are available for the analysis of the vasoactive properties of drugs with therapeutic applications. But all these models have limitations, such as ethical issues and high cost. The purpose of this study is to develop an alternative model for studying the vasoactive properties of drugs using an in-ovo chicken embryo model. In the preliminary experiment, we used a well-known vasoconstrictor (adrenaline) and a vasodilator (spermine NoNoate) in the chick embryo area vasculosa and evaluated their concentration-response curve. Adrenaline (10 µM) and spermine NoNoate (10 µM) were administered in different arteries and veins and different positions of the right vitelline artery of the chick embryo. Results showed the middle of the vessel bed of the right vitelline artery having the best vasoactive effect compared to others. Finally, anti-hypertensive drugs, calcium channel blockers, and NOS agonists were administered in the chick embryo area vasculosa to validate the model. Results demonstrate that the chick embryo area vasculosa can be an alternative, robust, and unique in-ovo model for screening of anti-hypertensive drugs in real time.
Offshore Postseismic Deformation of the 2011 Tohoku Earthquake Revisited: Application of an Improved GPS‐Acoustic Positioning Method Considering Horizontal Gradient of Sound Speed Structure
Authors: Honsho C. , Kido M. , Tomita F. , Uchida N. 
Published: May 2019

AbstractOne of the important issues on the GPS‐acoustic (GPS‐A) observation for sea bottom positioning is how to address the horizontal heterogeneity of the sound speed in oceans. This study presents an analysis method of GPS‐A data in the presence of a sloping sound speed structure. By applying this method and revising the analysis scheme to make full use of existing data, we reevaluated the horizontal postseismic deformations occurring ~1.5–5 years after the 2011 Tohoku earthquake. The revised horizontal movements have more uniform directions and rates between neighboring sites, suggesting enhancement of the array positioning accuracy. The revised displacement rate of the site on the incoming Pacific plate, located ~100 km northeast of the main rupture zone, was decreased significantly; it was only slightly, by 1.4 cm/year larger than the global motion of the Pacific plate, suggesting a relatively small effect of viscoelastic relaxation. The horizontal movements of the near‐trench sites above the main rupture zone were generally landward and were significantly faster than the Pacific plate motion, indicating a viscoelastic relaxation of 5–10 cm/year. The distribution of the fast landward movements peaked near 38°N at an updip of the mainshock hypocenter and extended significantly farther to the north than to the south. This implies the existence of a secondary coseismic slip patch in the northern area in addition to a primary slip patch at ~38°N. The occurrence of episodic slow slip in early 2015 to the north of the main rupture zone was also verified from the GPS‐A analyses.
Evolution of Dissolved Organic Matter Along a Septic System Plume: Evidence of Sustained Biogeochemical Activity in the Groundwater Zone
Authors: Aukes P. J. K. , Schiff S. L. , Robertson W. D. 
Published: May 2019

AbstractDissolved organic matter (DOM) is an important energy source for biogeochemical reactions. However, DOM is often assumed to be recalcitrant in the groundwater zone due to extensive microbial processing in the overlying soil zone and long groundwater residence times. Consequently, further heterotrophic microbial processes proceed at inherently low rates. Septic systems provide an ideal opportunity to study the evolution of groundwater DOM due to the development of confined and easily identifiable plumes of known age. Here we use measures of DOM composition (size‐exclusion liquid chromatography, ultraviolet absorbance, and C:N) to quantify DOM evolution along an exceptionally well‐characterized septic‐impacted groundwater plume. Elevated concentrations (5 to 8 mg C/L) close to the tile bed decrease to values below 2 mg C/L with lower UV‐absorbing components normalized to overall DOM concentration (specific ultraviolet absorbance) along the six‐year‐old plume flow path. The humic substance fraction (HSF) comprises the largest percentage of DOM and decreases in concentration along the septic plume. HSF properties also change with travel time becoming lower in molecular weight, specific ultraviolet absorbance, and C:N. Denitrification continues along the length of the plume concomitant with changes in DOM and HSF composition. Thus, HSF, typically considered recalcitrant in subsurface environments, is actively cycled by microorganisms on multiyear time frames in accordance with the recent paradigm shift for soil organic matter evolution. Lastly, measures of DOM composition indicate changes in DOM that are not evident from measures of concentration alone.
Trapped and Accelerated Electrons Within a Magnetic Mirror Behind a Flux Rope on the Magnetopause
Authors: Zhu Changbo , Zhang Hui , Fu Song , Ni Binbin , Strangeway R. J. , Giles B. L. , Wan Weixing , Liu Libo , Chen Yiding , Le Huijun 
Published: May 2019

AbstractOn 6 December 2015, the Magnetospheric Multiscale satellites traversed the southward outflow of a magnetic reconnection on the magnetopause from the outside in. The magnetic structures or the geometries of the field lines inside this outflow are distinguished in detail by using the plasma and magnetic field observations. A flux rope and the magnetic flux piling‐up against this flux rope are found embedding in this outflow. The strong magnetosheath magnetic field allows the formation of a magnetic mirror in the flux pileup region. As the Magnetospheric Multiscale satellites approached the flux rope inside this mirror, the pitch angles of electrons converge toward 90°, indicating that the electrons are trapped inside the mirror. The local loss cones of these electrons along the satellite trajectory are estimated. It is found that the triangle‐like pitch angle distribution is well embraced by the curves of the estimated local loss cones. The energy spectra inside the mirror show that the electrons are accelerated in the direction perpendicular to the magnetic field. A test particle method is used to reproduce the acceleration process in a fitted 3‐D magnetic field model. The results show that the quasi‐perpendicular electrons experience the betatron acceleration when drifting into the stronger field region and that the electrons may also experience the Fermi acceleration when their pitch angles enlarge. The mirror structure on the magnetopause provides a unique opportunity to explore the behaviors of the magnetic reconnection associated electrons.
Impact of Aseismic Ridges on Subduction Systems: Insights From Analog Modeling
Authors: Flórez‐Rodríguez A. G. , Schellart W. P. , Strak V. 
Published: May 2019

AbstractThe influence of aseismic ridges on subduction kinematics and dynamics, and on the deformation of the overriding plate, remains a topic of debate. This study presents laboratory‐based geodynamic models that simulate the process of aseismic ridge subduction below an overriding plate. The analog experiments show that, depending on its size, subduction of an aseismic ridge can impact on the kinematics of subduction, overriding plate deformation and, to a lower extent, on the geometry of the slab and that of the trench. Specifically, it can cause a reduction of the subducting plate and trench retreat velocities, decreases overriding plate extension and even drives local forearc shortening for the widest ridge, increases the slab dip angle next to the aseismic ridge, but decreases the dip angle at the aseismic ridge in case the ridge is thick and wide, and produces a local perturbation (indentation) of the trench geometry. The magnitude of the described modifications relies to a comparable extent on aseismic ridge width and thickness. The effects of aseismic ridge subduction observed in our models compare to those of the Louisville Seamount Chain, the Cocos Ridge, and the d'Entrecasteaux Ridge system in nature and provide an explanation for the local deformation observed at these subduction zones.
Ibrutinib induces chromatin reorganisation of chronic lymphocytic leukaemia cells
Authors: Holmes Katie B. , Sadreev Ildar I. , Rawstron Andy C. , Munir Tal , Westhead David R. , Hillmen Peter , Lefevre Pascal F. 
Published: May 2019

AbstractChronic lymphocytic leukaemia (CLL) is the most common leukaemia in Western countries. It has recently been shown that the homogeneity of the chromatin landscape between CLL cells contrasts with the important observed genetic heterogeneity of the disease. To gain further insight into the consequences of disease evolution on the epigenome’s plasticity, we monitored changes in chromatin structure occurring in vivo in CLL cells from patients receiving continuous Ibrutinib treatment. Ibrutinib, an oral inhibitor of the Bruton’s tyrosine kinase (BTK) has proved to be remarkably efficient against treatment naïve (TN), heavily pre-treated and high-risk chronic lymphocytic leukaemia (CLL), with limited adverse events. We established that the chromatin landscape is significantly and globally affected in response to Ibrutinib. However, we observed that prior to treatment, CLL cells show qualitative and quantitative variations in chromatin structure correlated with both EZH2 protein level and cellular response to external stimuli. Then, under prolonged exposure to Ibrutinib, a loss of the two marks associated with lysine 27 (acetylation and trimethylation) was observed. Altogether, these data indicate that the epigenome of CLL cells from the peripheral blood change dynamically in response to stimuli and suggest that these cells might adapt to the Ibrutinib “hit” in a process leading toward a possible reduced sensitivity to treatment.
Phosphorylation of 14-3-3ζ links YAP transcriptional activation to hypoxic glycolysis for tumorigenesis
Authors: Jia Yu , Li Hui-Yan , Wang Jue , Wang Ying , Zhang Peng , Ma Ning , Mo Shi-Jing 
Published: May 2019

AbstractHypoxic microenvironment deregulates metabolic homeostasis in cancer cells albeit the underlying mechanisms involved in this process remain hitherto enigmatic. 14-3-3ζ/Yes-associated protein (YAP) axis plays a principal role in malignant transformation and tumor development. Here, we report that hypoxia disassembles 14-3-3ζ from YAP and thereby promotes YAP nuclear localization mediated by ERK2, which directly binds to the D-site of mitogen-activated protein kinase (MAPK) docking domain in 14-3-3ζ Leu98/100 and phosphorylates 14-3-3ζ at Ser37. When localizing in nucleus, YAP recruits at pyruvate kinase M2 (PKM2) gene promoter with hypoxia-inducible factor 1α (HIF-1α), for which PKM2 transcription is required. 14-3-3ζ Ser37 phosphorylation is instrumental for the hypoxia-induced glucose uptake, lactate production, and clonogenicity of pancreatic ductal adenocarcinoma (PDAC) cells, as well as tumorigenesis in mice. The 14-3-3ζ Ser37 phosphorylation positively correlates with p-ERK1/2 activity and HIF-1α expression in clinical samples from patients with PDAC and predicts unfavorable prognosis. Our findings underscore an appreciable linkage between YAP transcriptional activation and hypoxic glycolysis governed by ERK2-dependent 14-3-3ζ Ser37 phosphorylation for malignant progression of PDAC.
TRAF4 positively regulates the osteogenic differentiation of mesenchymal stem cells by acting as an E3 ubiquitin ligase to degrade Smurf2
Authors: Li Jinteng , Wang Peng , Xie Zhongyu , Wang Shan , Cen Shuizhong , Li Ming , Liu Wenjie , Tang Su’an , Ye Guiwen , Zheng Guan , Su Hongjun , Ma Mengjun , Wu Xiaohua , Wu Yanfeng , Shen Huiyong 
Published: May 2019

AbstractTNF receptor-associated factor 4 (TRAF4), a member of the TRAF family, plays an important role in the embryogenesis and development of the bone system. Mesenchymal stem cells (MSCs), which are the primary origin of osteoblasts in vivo, are key cells in bone development; however, whether TRAF4 modulates the osteogenic capacity of MSCs has never been explored. In this study, we demonstrated that TRAF4 positively regulates the osteogenic process of MSCs both in vitro and in vivo. In addition, we further demonstrated that TRAF4 modulates the osteogenic process of MSCs by acting as an E3 ubiquitin ligase to mediate the K48-linked ubiquitination of Smurf2 at the K119 site and cause degradation. Furthermore, TRAF4 was abnormally decreased in bone sections of ovariectomized rat and osteoporosis patients. Taken together, our findings suggest that TRAF4 positively regulates the osteogenic differentiation of MSCs by acting as an E3 ubiquitin ligase to degrade Smurf2. These results emphasize the critical role of TRAF4 in bone formation and could not only improve the clinical use of MSCs in tissue engineering but also clarify the pathogenesis of bone metabolism disorders.
Low-dose aspirin protective effects are correlated with deregulation of HNF factor expression in the preeclamptic placentas from mice and humans
Authors: Ducat Aurélien , Vargas Alexandra , Doridot Ludivine , Bagattin Alessia , Lerner Jonathan , Vilotte Jean-Luc , Buffat Christophe , Pontoglio Marco , Miralles Francisco , Vaiman Daniel 
Published: May 2019

AbstractAspirin (acetyl-salicylic acid) is one of the most ancient drugs of the human pharmacopeia. Nonetheless, its action at low doses is not well understood at the molecular level. One of the applications of low-dose aspirin treatment is the prevention of preeclampsia (PE) in patients at risk. Foeto-placental overexpression of the STOX1A transcription factor in mice triggers PE symptoms. Transcriptomic analysis of the placentas, showed that aspirin massively down-regulates genes of the coagulation and complement cascade, as well as genes involved in lipid transport. The genes modified by aspirin treatment are not the ones that are modified by STOX1 overexpression, suggesting that aspirin could act downstream, symptomatically on the preeclamptic disease. Bioinformatics analysis of the promoters of the deregulated genes showed that they are strongly enriched in HNF transcription factors-binding sites, in accordance with existing literature showing their roles as regulators of coagulation. Two of these transcription factors, Hnf1β and Hnf4α are found down-regulated by aspirin treatment. In parallel, we show that in human patient placentas, aspirin-induced deregulations of genes of the coagulation cascade are also observed. Finally, the expression of Hnf1β target sequences (Kif12, F2, Hnf4α promoters and a synthetic concatemer of the Hnf1β-binding site) were investigated by transfection in trophoblast cell models, with or without aspirin treatment and with or without STOX1A overexpression. In this model we observed that STOX1A and aspirin tended to synergize in the down-regulation of Hnf1β target genes in trophoblasts.