bluuebunny/crossref_metadata_2025_split huggingface dataset:
The guardians of the periodontium—sequential and differential expression of antimicrobial peptides during gingival inflammation. Results from in vivo and in vitro studies
Authors: Dommisch Henrik , Skora Philipp , Hirschfeld Josefine , Olk Gabriela , Hildebrandt Laura , Jepsen Søren 
Published: February 2019

AbstractAimTo evaluate the sequential and differential expression of a variety of antimicrobial peptides (AMPs) during the development of an experimentally induced gingivitis in humans.Material and methodsIn twenty healthy volunteers, gingival inflammation was induced by abstention from oral hygiene at 6 teeth. Bleeding on probing (BOP) and plaque index (PI) were assessed, and gingival biopsies and gingival crevicular fluid (GCF) were collected at 8 different time points (t0‐t35). Gingival epithelial cells (GECs) were stimulated with various receptor agonists. In biopsies and GECs, mRNA expression of human beta‐defensins (hBD‐2, hBD‐3), CC‐chemokine ligand 20 (CCL20), S100A7/psoriasin (S100A7), and calgranulin A/B (S100A8, S100A9) was evaluated using real‐time PCR, and protein profiles were measured by ELISA. Statistical analysis was performed using non‐parametric tests.ResultsThe clinical parameters BOP, PI and GCF increased over time (p < 0.0001). Tissue AMP mRNA expression was elevated, but at different and AMP‐specific time points (p < 0.05). Protein analysis revealed a similar expression pattern for hBD‐2 and CCL20 in GCF (p < 0.05). In GECs, multiple receptor stimulation was required to induce AMP gene expression (p < 0.0001).ConclusionsFor the first time, this study showed the sequential and differential expression of AMPs during a developing inflammation in vivo providing further evidence for their role as guardians of a healthy periodontium.
Randomized controlled trial of the effect of periodontal treatment on cardiovascular risk biomarkers in patients with stable coronary artery disease: Preliminary findings of 3 months
Authors: Montenegro Marlon M. , Ribeiro Ingrid W. J. , Kampits Cassio , Saffi Marco A. L. , Furtado Mariana V. , Polanczyk Carisi A. , Haas Alex N. , Rösing Cassiano K. 
Published: February 2019

AbstractAimTo assess the effect of periodontal therapy (PT) on cardiovascular blood biomarkers.Materials and MethodsThis single‐blind, parallel‐design, randomized controlled trial included patients with stable coronary artery disease and periodontitis. The test group (TG) received non‐surgical PT, whereas the control group (CG) received one session of plaque removal. Plasma levels of C‐reactive protein (CRP), glycated haemoglobin, lipids and cytokines (IL‐1β, IL‐6, IL‐8, IL‐10, IFN‐γ and TNF‐α) were measured at baseline and after 3 months.ResultsEighty‐two patients (74.4% women, mean age 59.6 years) were analysed. TG had significantly better periodontal parameters than CG after 3 months, but no significant differences in blood markers were observed between them. In a post hoc subgroup analysis in patients with baseline CRP <3 mg/L, a significant increase in CRP was observed in CG (1.44 ± 0.82 mg/L to 4.35 ± 7.85 mg/L, p = 0.01), whereas CRP remained unchanged in TG (1.40 ± 0.96 mg/L to 1.33 ± 1.26 mg/L, p = 0.85), resulting in a significant difference between groups at 3 months. In patients with CRP ≥3 mg/L, a significant reduction in CRP was observed only in TG (11.3 ± 12.8 mg/L to 5.7 ± 4.1 mg/L, p = 0.04). Levels of IL‐6 and IL‐8 were significantly lower in TG than CG at 3 months.ConclusionsPT leads to lower levels of CRP, IL‐6 and IL‐8 in cardiovascular patients with high CRP levels.
Multiple recurrences after endoscopic removal of common bile duct stones: A retrospective analysis of 976 cases
Authors: Kawaji Yuki , Isayama Hiroyuki , Nakai Yousuke , Saito Kei , Sato Tatsuya , Hakuta Ryunosuke , Saito Tomotaka , Takahara Naminatsu , Mizuno Suguru , Kogure Hirofumi , Matsubara Saburo , Tada Minoru , Kitano Masayuki , Koike Kazuhiko 
Published: February 2019

AbstractBackground and AimRecurrences after endoscopic treatment of common bile duct stones (CBDS) are common. The aims of this study were to identify risk factors for recurrences of CBDS and to evaluate the effect of interventions for prevention of further recurrences.MethodsA total of 976 patients who underwent endoscopic treatment of CBDS were retrospectively studied. Risk factors for single and multiple recurrent CBDS were evaluated using a Cox hazard regression model. The incidences of further recurrences were evaluated according to the additional interventions.ResultsThe mean age was 69.3 years, and 39.3% were female. Endoscopic papillary balloon dilation, endoscopic sphincterotomy, and endoscopic papillary large balloon dilation were performed in 858, 77, and 41 patients, respectively. The rates of one or more recurrence and multiple recurrences of CBDS were 12.4% and 2.7%, respectively. In the multivariate analyses, the significant risk factors were the bile duct size (hazard ratio [HR] 1.07, P = 0.012), gallbladder left in situ with stones (HR 1.91, P = 0.046), and pneumobilia after treatment (HR 2.10, P = 0.047) for single recurrence and the number of stones at the first recurrence (HR 1.16, P = 0.021) for multiple recurrences. In five out of nine cases with multiple recurrences, further recurrence was not observed after additional sphincteroplasty in addition to cholecystectomy.ConclusionsThe incidence of multiple recurrences was not uncommon after the first recurrence of CBDS.
PEX16 contributions to peroxisome import and metabolism revealed by viable Arabidopsis pex16 mutants
Authors: Burkhart Sarah E. , Llinas Roxanna J. , Bartel Bonnie 
Published: February 2019

AbstractPeroxisomes rely on peroxins (PEX proteins) for biogenesis, importing membrane and matrix proteins, and fission. PEX16, which is implicated in peroxisomal membrane protein targeting and forming nascent peroxisomes from the endoplasmic reticulum (ER), is unusual among peroxins because it is inserted co‐translationally into the ER and localizes to both ER and peroxisomal membranes. PEX16 mutations in humans, yeast, and plants confer some common peroxisomal defects; however, apparent functional differences have impeded the development of a unified model for PEX16 action. The only reported pex16 mutant in plants, the Arabidopsis shrunken seed1 mutant, is inviable, complicating analysis of PEX16 function after embryogenesis. Here, we characterized two viable Arabidopsis pex16 alleles that accumulate negligible PEX16 protein levels. Both mutants displayed impaired peroxisome function − slowed consumption of stored oil bodies, decreased import of matrix proteins, and increased peroxisome size. Moreover, one pex16 allele exhibited reduced growth that could be alleviated by an external fixed carbon source, decreased responsiveness to peroxisomally processed hormone precursors, and worsened or improved peroxisome function in combination with other pex mutants. Because the mutations impact different regions of the PEX16 gene, these viable pex16 alleles allow assessment of the importance of Arabidopsis PEX16 and its functional domains.
Does lymphadenectomy effect postoperative surgical morbidity and survival in patients with adult granulosa cell tumor of ovary?
Authors: Erkılınç Selçuk , Taylan Enes , Karataşlı Volkan , Uzaldı İpek , Karadeniz Tuğba , Gökçü Mehmet , Sancı Muzaffer 
Published: February 2019

AbstractAimTo evaluate the effect of lymphadenectomy on surgical morbidity and survival in adult granulosa cell tumor (AGCT) of the ovary.MethodsPatients who underwent surgical treatment for AGCT between January 1993 and January 2016 were identified. Data were collected for patient age, menopausal status, surgical staging, lymphadenectomy, postoperative complications (anemia, wound infection, incisional hernia), length of hospital stay, follow‐up duration, site and time for recurrence, management of recurrence and vital status. Histopathological records were also evaluated for number of cellular mitosis.ResultsLymphadenectomy (pelvic–paraaortic) was performed in 53 (53%) of 98 patients. Decrease in postoperative hemoglobin level and increased wound infection and longer hospital stay were significantly higher in lymphadenectomy group (P = 0.003, 0.043 and <0.001, respectively). Tumor stage (HR 95% CI 14.9 [2.43–92.8]) and number of mitoses >5 (HR 95% CI 14.9 [2.43–92.8]) were significantly associated with recurrence (P = <0.001 and 0.02, respectively). Tumor stage was the only prognostic factor for predicting overall survival (HR 95% CI 8.47 [2.17–33.2]). Lymphadenectomy showed no effect on disease‐free survival and overall survival both in multivariate Cox regression analyses (P = 0.46 and 0.69, respectively). Disease‐free survival and overall survival were similar in lymphadenectomy and no lymphadenectomy groups (Log Rank P = 0.382, 0.741, respectively).ConclusionLymphadenectomy had no improved effect on survival and had negative effect on surgical morbidity in patients with AGCT.
Management of vulvar lymphedema of filarial origin in a pregnant woman and its outcome: A case report
Authors: Dorairajan Gowri , Subbaiah Murali S. , Shanbhag Esha R. 
Published: February 2019

AbstractLymphedema of filarial origin affecting the vulva is extremely rare. It is a dilemma if seen in a pregnant woman as there are no guidelines regarding excision and the mode of delivery. With the World Health Organization‐driven global program to eradicate filaria, it is unfortunate to see such cases. We report of a woman who had massive lymphedema of both the labia majora following filarial infection with a small secondary ulcer. She presented to our outpatient department in early pregnancy. After detailed counseling with the couple, a decision was taken for excision. The same was carried out. The wound healed well but the lymphedema recurred after 6 weeks. She was thereafter managed symptomatically. Pregnancy advanced without any complication. Her intrapartum management for a successful vaginal delivery is outlined in the report.
Long‐term efficacy and safety of biosimilar infliximab (CT‐P13) after switching from originator infliximab: open‐label extension of the NOR‐SWITCH trial
Authors: Goll G. L. , Jørgensen K. K. , Sexton J. , Olsen I. C. , Bolstad N. , Haavardsholm E. A. , Lundin K. E. A. , Tveit K. S. , Lorentzen M. , Berset I. P. , Fevang B. T. S. , Kalstad S. , Ryggen K. , Warren D. J. , Klaasen R. A. , Asak Ø. , Baigh S. , Blomgren I. M. , Brenna Ø. , Bruun T. J. , Dvergsnes K. , Frigstad S. O. , Hansen I. M. , Hatten I. S. H. , Huppertz‐Hauss G. , Henriksen M. , Hoie S. S. , Krogh J. , Midtgard I. P. , Mielnik P. , Moum B. , Noraberg G. , Poyan A. , Prestegård U. , Rashid H. U. , Strand E. K. , Skjetne K. , Seeberg K. A. , Torp R. , Ystrøm C. M. , Vold C. , Zettel C. C. , Waksvik K. , Gulbrandsen B. , Hagfors J. , Mørk C. , Jahnsen J. , Kvien T. K. 
Published: February 2019

AbstractBackground and objectivesThe 52‐week, randomized, double‐blind, noninferiority, government‐funded NOR‐SWITCH trial demonstrated that switching from infliximab originator to less expensive biosimilar CT‐P13 was not inferior to continued treatment with infliximab originator. The NOR‐SWITCH extension trial aimed to assess efficacy, safety and immunogenicity in patients on CT‐P13 throughout the 78‐week study period (maintenance group) versus patients switched to CT‐P13 at week 52 (switch group). The primary outcome was disease worsening during follow‐up based on disease‐specific composite measures.MethodsPatients were recruited from 24 Norwegian hospitals, 380 of 438 patients who completed the main study: 197 in the maintenance group and 183 in the switch group. In the full analysis set, 127 (33%) had Crohn's disease, 80 (21%) ulcerative colitis, 67 (18%) spondyloarthritis, 55 (15%) rheumatoid arthritis, 20 (5%) psoriatic arthritis and 31 (8%) chronic plaque psoriasis.ResultsBaseline characteristics were similar in the two groups at the time of switching (week 52). Disease worsening occurred in 32 (16.8%) patients in the maintenance group vs. 20 (11.6%) in the switch group (per‐protocol set). Adjusted risk difference was 5.9% (95% CI −1.1 to 12.9). Frequency of adverse events, anti‐drug antibodies, changes in generic disease variables and disease‐specific composite measures were comparable between arms. The study was inadequately powered to detect noninferiority within individual diseases.ConclusionThe NOR‐SWITCH extension showed no difference in safety and efficacy between patients who maintained CT‐P13 and patients who switched from originator infliximab to CT‐P13, supporting that switching from originator infliximab to CT‐P13 is safe and efficacious.
The Psychological Management of the Poor: Prescribing Psychoactive Drugs in the Age of Neoliberalism
Authors: Dougherty Ryan J 
Published: February 2019

AbstractThis article examines neuroleptics in relation to the histories of biopsychiatry and neoliberalism in the United States. Drawing from Foucault's concept of biopower, I contend that neuroleptics are socially constructed as a mechanism to address underlying biological illnesses in order to achieve neoliberal subjectivity for mad/disabled people. I then argue this biopsychiatric and neoliberal construct dominates services with the expressed goal of creating people who self‐govern their own drug consumption. This, however, contrasts with accounts that depict intersubjective balancing acts between treatment providers and mad/disabled people, who must incorporate the various effects of psychoactive drugs beyond measures of rehabilitation. In doing so, I advocate for new lines of psychological inquiry that critically examine the structural determinants of poverty and the underlying constructions of citizenship and wellness.
Natural antimicrobials for control of Salmonella Enteritidis in feed and in vitro model of the chicken digestive process
Authors: Oliveira Geovane R. , Oliveira Willian K. , Andrade Carla , Melo Antonio Diego B. , Luciano Fernando B. , Macedo Renata E. F. , Costa Leandro B. 
Published: February 2019

AbstractThis study evaluated the antimicrobial effect of essential oils (EO) and organic acids (OA) against Salmonella Enteritidis in chicken feed and during an in vitro model that mimics the chicken digestive process. The minimal inhibitory concentration (MIC) of allyl isothiocyanate (AITC), carvacrol (CV), propionic acid (PROP) and caproic acid (CAP) were individually determined. Then, based on the MICs of each compound, combinations of EOs and/or OAs were tested to evaluate their synergic antimicrobial effect. The synergic effect of AITC and CAP was the most efficient against the bacterial strain tested. Commercial feed was inoculated with a 5‐strain cocktail of S. Enteritidis and treated with different doses of AITC + CAP to evaluate their effect on the growth/survival of the pathogen. In addition, the simulated digestion model was used to access the antimicrobial effect of AITC + CAP added to the feed towards S. Enteritidis and Lactobacillus plantarum. Synergistic effect was found between AITC (0.065 mM) and CAP (17.5 mM) against S. Enteritidis in chicken feed, where S. Enteritidis was reduced to undetectable levels (<1.00 log CFU/g). AITC (1.95 mM) + CAP (45 mM) also decreased (p < 0.05) the population of S. Enteritidis in the simulated digestion, while the growth of L. plantarum was not affected. Therefore, the addition of AITC + CAP in feed might be a potential natural antimicrobial able to prevent economic losses caused for Salmonella in chicken.
Fire and lichen dynamics in the Taiga Shield of the Northwest Territories and implications for barren‐ground caribou winter forage
Authors: Lewis Kathy J. , Johnson Chris J. , Karim MD. Nayeem 
Published: February 2019

AbstractQuestionsFire is the main disturbance agent in boreal forests and has profound effects on vegetation composition and structure, including terrestrial lichens that provide critical winter forage for barren‐ground caribou. What is the influence of fire on forest structure and the distribution and species assemblage of forage lichens? What is the current or potential effect of climate change on forage lichen in the boreal forest?LocationHigh Boreal, Low Subarctic, and High Subarctic ecoregions of the Taiga Shield Ecozone in the Northwest Territories, Canada.MethodsWe used fire history data, satellite and air photo images, and field sampling to determine fire history and severity, and subsequent effects on tree and lichen species composition, in a 880,000 ha study area in the winter range of the Bathurst caribou herd.ResultsWe found that low‐ and mixed‐severity fires were more common than high‐severity fires, and that forest fire severity increased with distance to treeline. Forest structure and composition were more important drivers of lichen cover than fire severity or time since fire.ConclusionsWhile fire had a dominant role in forest structure and composition, the effects on lichen availability were due more to variation in tree growth. We found little evidence of changes in fire regime over the length of our reconstructions (approximately 200 years); however, increases in temperature and the positive relationship between temperature and fire frequency may lead to shortened fire cycles causing a reduction in the availability of forage lichens through burning.
Bosumtwi impact structure, Ghana: Evidence for fluidized emplacement of the ejecta
Authors: Baratoux David , Niang Cheikh Ahmadou Bamba , Reimold Wolf Uwe , Sapah Marian Selorm , Jessell Mark Walter , Boamah Daniel , Faye Gayane , Bouley Sylvain , Vanderhaeghe Olivier 
Published: February 2019

AbstractThe about 10.5 km diameter Bosumtwi impact crater is one of the youngest large impact structures on Earth. The crater rim is readily noticed on topographic maps or in satellite imagery. It defines a circular basin filled by water (Lake Bosumtwi) and lacustrine sediments. The morphology of this impact structure is also characterized by a circular plateau extending beyond the rim and up to 9–10 km from the center of the crater (about 2 crater radii). This feature comprises a shallow ring depression, also described as an annular moat, and a subdued circular ridge at its outer edge. The origin of this outermost feature could so far not be elucidated based on remote sensing data only. Our approach combines detailed topographic analysis, including roughness mapping, with airborne radiometric surveys (mapping near‐surface K, Th, U concentrations) and field observations. This provides evidence that the moat and outer ring are features inherited from the impact event and represent the partially eroded ejecta layer of the Bosumtwi impact structure. The characteristics of the outer ridge indicate that ejecta emplacement was not purely ballistic but requires ejecta fluidization and surface flow. The setting of Bosumtwi ejecta can therefore be considered as a terrestrial analog for rampart craters, which are common on Mars and Venus, and also found on icy bodies of the outer solar system (e.g., Ganymede, Europa, Dione, Tethys, and Charon). Future studies at Bosumtwi may therefore help to elucidate the mechanism of formation of rampart craters.
Advancing the Scientific Understanding of Trust and Culture in Negotiations
Authors: Kong Dejun Tony , Yao Jingjing 
Published: February 2019

AbstractTrust plays a crucial role throughout the entire negotiation process, and culture adds more complexity to the meaning, functions, and dynamics of trust in negotiations. We take a modest step to provide some insights on trust and culture in the context of negotiations and envision what opportunities are ahead of us in this area. Specifically, we provide a “cognitive map” based on the collective wisdom in the extant negotiation literature and focus on raising important questions about six key culture‐related issues that warrant future research: (a) the meaning of trust, (b) the effects of trust, (c) trust development, (d) trust and distrust, (e) trust repair, and (f) trust in virtual negotiations.
Comparative analysis reveals Ce3D as optimal clearing method for in toto imaging of the mouse intestine
Authors: Bossolani Gleison D. P. , Pintelon Isabel , Detrez Jan D. , Buckinx Roeland , Thys Sofie , Zanoni Jacqueline Nelisis , De Vos Winnok H. , Timmermans Jean‐Pierre 
Published: February 2019

AbstractBackgroundThe intestinal wall has a complex topographical architecture. The multi‐layered network of the enteric nervous system and its intercellular interactions are difficult to map using traditional section‐based or whole‐mount histology. With the advent of optical clearing techniques, it has become feasible to visualize intact tissue and organs in 3D. However, as yet, a gap still needs to be filled in that no in‐depth analysis has been performed yet on the potential of different clearing techniques for the small intestine.AimThe goal of this study was to identify an optimal clearing protocol for in toto imaging of mouse intestinal tissue.MethodsFive aqueous‐based clearing protocols (SeeDB2, CUBIC, ScaleS, Ce3D, and UbasM) and four organic reagent‐based clearing protocols (3DISCO, iDISCO+, uDISCO, and Visikol®) were assessed in segments of small intestine from CX3CR1GFP/GFP and wild‐type mice. Following clearing, optical transparency, tissue morphology, green fluorescent protein (GFP) fluorescence retention, and compatibility with (immuno‐)labeling were analyzed.Key resultsAll organic reagent‐based clearing protocols—except for Visikol—rendered tissue highly transparent but led to substantial tissue shrinkage and deformation. Of the aqueous‐based protocols, only Ce3D yielded full‐thickness tissue transparency. In addition, Ce3D displayed excellent GFP retention and preservation of tissue morphology.ConclusionsCe3D emerged as a most efficient protocol for enabling rapid full‐thickness 3D mapping of the mouse intestinal wall.
Tree mycorrhizal associations mediate soil fertility effects on forest community structure in a temperate forest
Authors: Mao Zikun , Corrales Adriana , Zhu Kai , Yuan Zuoqiang , Lin Fei , Ye Ji , Hao Zhanqing , Wang Xugao 
Published: February 2019

Summary

Soil fertility influences plant community structure, yet few studies have focused on how this influence is affected by the type of mycorrhizal association formed by tree species within local communities.

We examined the relationship of aboveground biomass (AGB) and diversity of adult trees with soil fertility (nitrogen, phosphorus, organic matter, etc.) in the context of different spatial distributions of arbuscular mycorrhizal (AM) and ectomycorrhizal (EM) trees in a temperate forest in Northeast China.

Diversity showed a positive trend along the soil fertility gradient driven mostly by a positive relationship between AM tree abundance and soil fertility. By contrast, the AGB showed a negative trend along the soil fertility gradient driven mostly by a negative relationship between EM tree AGB and soil fertility. Furthermore, the opposite trend in the AGB and tree species diversity along the soil fertility gradient led to an overall negative diversity–biomass relationship at the 50‐m scale but not the 20‐m scale.

These results suggest that tree mycorrhizal associations play a critical role in driving forest community structure along soil fertility gradients and highlight the importance of tree mycorrhizal associations in influencing how the diversity–ecosystem function (e.g. biomass) relationships change with soil fertility.
Childhood pemphigus foliaceus presenting as a polycyclic eruption: Case report and review of the literature
Authors: Evans Megan S. , Culton Donna A. , Diaz Luis A. , Googe Paul B. , Morrell Dean S. 
Published: February 2019

AbstractPemphigus foliaceus (PF) is an autoimmune bullous disorder that has occasionally been reported to present as a polycyclic or arcuate eruption in children. We present a case of childhood PF presenting as an annular and polycyclic eruption, which initially led to a diagnostic conundrum and a delay in diagnosis but which ultimately responded well to therapy with systemic steroids and rituximab infusions. We briefly review the literature on polycyclic presentations of PF in childhood as well as the use of rituximab for pediatric pemphigus.