bluuebunny/crossref_metadata_2025_split huggingface dataset:
PO-088 Effect of Aerobic Exercise on Myocardial Mitochondria Fusion-Fission in Chronic Intermittent Hypoxia Mice
Authors: Hu Yulong , Chen Juan 
Published: October 2018

Objective Training monitoring is an important part of scientific training, and also accumulated a large amount of data, but the analysis and evaluation of biochemical indicators are mostly concentrated on the level of experience and the general, phased and individualized research application of statistical methods. The data mining technology is applied to the analysis and evaluation of the biochemical indexes of competitive sports, the analysis of the data is carried out in the deep level, the potential, new and useful information and knowledge are extracted, and the new exploration ideas are carried out for the analysis of the biochemical indexes of competitive sports, and a more reliable and more powerful data branch is provided for the scientific and efficient training support.
Methods Using the literature data method, logic analysis method and expert interview method, the application of the current data mining technology in the analysis of biochemical indicators is summarized.
Results The scientific analysis and evaluation of athletes' physical function status has been the focus of domestic and foreign coaches and sports researchers. The application of data mining technology in sports biochemical indicators is also becoming more and more extensive. For example, Mao Jie and others applied the gray ART clustering model analysis method to the monitoring of competitive sports biochemical indicators. Through this data mining model, the coach can easily judge the athlete's competitive physical condition, and can provide a scientific basis for correct training according to the different competitive conditions of each athlete, using different training guidance programs and training methods. Ma Jing et al. explored the feasibility of applying decision tree algorithm and association rules in volleyball biochemical analysis. It was found that C5.0 decision tree and Apriori association rule algorithm can be used to predict and analyze the technical level of women's volleyball players. Li Guangjun and others successfully applied the association rule data mining to the biochemical data analysis of canoeists, and provided a basis for scientific decision-making and analysis of sports training and athlete selection. Zhang Hui designed a data mining system for sports biochemical index based on association rules. The results show that the system has fast data mining rate, short time consuming and high reliability. It provides a more scientific evaluation standard for the data mining of sports biochemical index, and also provides a basis for the future training program.
Conclusions With the development of competitive sports, in order to achieve new heights, the application of data mining technology to vast biochemical data is of great significance for the establishment of scientific training evaluation methods and standards, and is also the inevitable development of future sports scientific research.
PO-123 Voluntary wheel training attenuate brain aging in SAMP8 mice
Authors: Chen Dandan , Kou Xianjuan 
Published: October 2018

Objective Alzheimer's disease (AD), is a progressive neurodegenerative disease. The pathological hallmarks including the deposition of senile plaque and neurofibrillary tangles (NFTs) in AD. The underlying molecular mechanisms for AD are not fully understood. Studies have shown that exercise as an economical and popular fitness method, long-term exercise can reduce the risk of AD, delay the occurrence of AD or delay the progression of AD. This study was to investigate the protective effect of the Voluntary wheel on cerebral cortex senescence in SAMP8 mice.
Methods Fifteen SPF-class 7-month-old SAMP8 mice were randomly divided into control group (CON), voluntary wheel group (V) and voluntary wheel with chloroquine group (VQ), with 5 mice in each group. Each group was under adaptive feeding for one week. After 3 days of adaptive voluntary wheel training for V and VQ groups, the experiment officially started. The V and VQ groups performed an 8-week voluntary wheel training and the VQ group received a daily intraperitoneal injection of chloroquine (40 mg/kg). After 8 weeks intervention, the Morris water maze test was used to determine the changes in spatial learning and memory ability of each group. After 24 hours of the end of this experiment, the mice were killed by breaking neck. The degree of hippocampal cell injury was detected by Nissl staining; the expression of apoptosis and senescence-associated protein in the cerebral cortex was detected by Western blot.
Results Compared with the CON group, the escape latency of the V group mice in the Morris water maze test was significantly shorter (P<0.01), the number of crossing target quadrants was significantly increased (P<0.05), however, the VQ group exacerbate above response compared with CON group. Nissl staining showed that the cells of cerebral cortex in the CON group had obvious damage, while the V group showed significant improvement. The VQ group had a significant damage compared with the V group; Western blot results showed that the expression of AD -like pathological changes such as BACE1 protein in the cortex of group V was significantly decreased, and the expression of P-GSK-3β protein was increased (P<0.01) compared with CON group. On the contrary, compared with V group, VQ alleviated the improvement effect of voluntary running. (P<0.05); the expression of apoptosis-related protein increased, and thedecreased in CON group , while voluntary running intervention down-regulated BAX, Cleaved-caspase3 , increased the Bcl-2 protein level compared with the CON group (P<0.05). Furthermore, compared with the CON group, the expression level of aging-related protein AC-P53 was decreased in the V group, and the expression level of Sirt1 was increased.
Conclusions The 8-week voluntary wheel training can significantly improve the learning and memory ability of SAMP8. Mechanismly, voluntary wheel training can improve the senescence of cerebral cortex by inhibiting cell apoptosis, the expression of AD –like pathological protein and increasing Sirt1 protein level.
PO-141 The cardiovascular protection of irisin and its research progress in sport field
Authors: Wang Shen , Hu Min 
Published: October 2018

Objective The irisin, recently identified novel molecule, exercise induced myokinehas，has been shown to be secreted from fibronectin type III domain containing 5 (FNDC5) of skeletal muscle by an unknown protease. It can drive brown-fat-like development of white fat, increase thermogenesis and lose weight. Apart from this, its expression and role in various other conditions such as inflammation, hippocampal neurogenesis, aging and other metabolic conditions have been reported. Moreover, it has benn reported that irisin play an important role in the regulation of various cardiovascular disease, such as endothelial disfunction, hypertension and atherosclerosis. The present review discussed the research progress of irisin in the field of sports, and the protective effects for cardiovascular disease. However, due to conflicting results, several issues have been raised regarding its expression, cleavage, circulating levels, detection, the form of exercise, etc. And it also discussed the current challenges and future perspectives.
Methods Complete literature survey was performed using PubMed and WOS database search to gather available information regarding FNDC5/irisin.
Results The present review discussed on the discovery of irisin, its possible role in the cardiovascular protection and its research progress in sport field. It provide a research direction and new perspective of the possible target for the prevention and treatment of related disease.
Conclusions Irisin has a promising effect in predicting and diagnosing cardiovascular diseases, and the exercise level could be a influence factor. More research will be needed in the future.
PO-146 Effects of aerobic exercise training on GTT and ITT in apelin Knockout mice
Authors: He Shiyi , Zhang Ying 
Published: October 2018

Objective Aerobic exercise training is important to prevent and cure chronic diseases such as diabetes, cardiovascular diseases and so on. Apelin has been identified as a novel myokine in recent years, and the exogenous supplementation of apelin can promote the glucose absorption, the biosynthesis of mitochondria and the oxidation of fatty acids in skeletal muscle. Intraperitoneal glucose (GTT) and insulin tolerance tests (ITT) are useful in vivo assays that provide approximations of glucose metabolism and homeostasis. The bigger area under the curve (AUC) confirmed the decreased glucose clearance, which is evaluated by GTT. However, the mechanism of apelin mediating glucose metabolism during aerobic exercise training is not clear. Our study was to investigate the differences of GTT and ITT after four weeks training between wild-type (WT) mice (C57BL/6J) and apelin Knockout (KO) mice.
Methods Two-month-old WT and KO were divided into trained and control groups (n=8-10/group) respectively. There are four groups: WT control (WC), apelin KO control (KC), WT trained (WT), and apelin KO trained (KT). The trained groups were trained on treadmills for four weeks (six days per week and one hour per day). In order to maintain the exercise intensity, the speed is at 70%-75% VO2max with an incline of 5 degrees. The control groups were kept at a sedentary condition. after four weeks of interventions, glucose was measured at 0, 15, 30, 45, 60, 90, 120min following GTT. Glucose was also measured at 0, 30, 60, 90, 120min following ITT.
Results (1) blood glucose levels and AUC of the KC were significantly bigger than those of WC. ITT showed that KC also had slower insulin-stimulated glucose clearance compared with the WC. (2) Following 4-week training, KT had lower blood glucose levels and AUC of the KT was significantly smaller than those of KC. KT had faster insulin-stimulated glucose clearance compared with KC.
Conclusions Without apelin, glucose tolerance and insulin tolerance in mice will decrease. And aerobic exercise training improves them in apelin deficiency mice.
PO-147 Effect of Exercise-induced Fatigue on the Electrical Activity of the External Globus Pallidus Neurons in rats
Authors: Yu Hao , Hou Lijuan , Ma Jing , Wang Zhifeng , Zhao Gang , Qiao Decai 
Published: October 2018

Objective The motor cortex (MC) stimulation-induced unitary responses of globus pallidus external segment (GPe) neurons in control and exercise induced-fatigue rats were recorded in vivo to examine the role of cortical-striatum-external globus pallidal pathway in the mechanism of central fatigue.
Methods 32 Clean healthy male Wistar rats (260~300g), were randomly divided into 4 groups: control group (Control), 1-day fatigue group (1FG), 3-day fatigue group (3FG) and 7-day fatigue group (7FG). Rats were subjected to a 5-day adaptive treadmill training. Modified Bedford treadmill exercise with progressively increasing load was used to creat the exercise fatigue model. （3 levels:8.2 m/min, 15 min; 15m/min, 15 min; 20 m/min, lasting till exhaustion） The spontaneous unit activity and responses to MC stimulation of GPe neurons were recorded by the electrophysiological technique of extracellular recording of glass microelectrodes.
Results The results showed that the firing frequency of high-frequency firing with pause (HFP) and low frequency firing with burst (LFB) in the GPe of 1FG was comparable with that of control group (P>0.05). However in 3FG and 7FG , the percentage of HFP neuron was significantly decreased (P<0.05), while the proportion of LFB was significantly increased (P<0.05), and the average firing rate of LFB was higher and inter spike intervals (ISI) was significantly lower than that of the control group. With 200μA electrical stimulation, the explosive discharge of GPe neurons was attenuated after fatigue in rats. The response of GPe neurons to variable frequency stimulation in exhausted model groups was stronger than that of the control group.MC-stimulation typically induced a triphasic response composed of early excitation, inhibition, and late excitation in GPe neurons. The population of neurons showing a short inhibition slightly increased in 3FG and 7FG.
Conclusions 1. The results confirmed that GPe is an important nucleus of basal ganglia involved in the regulation of exercise-induced fatigue by the change of spontaneous activity.

Electrical stimulation on the cortex can alter response patterns of GPe neurons in exercise-induced fatigue rats, the results confirmed that the Ctx-Str-GPe neural pathway is involved in the regulation of exercise fatigue, and the indirect pathway is over-activated.
PO-148 Effect of Aerobic Exercise on the Expression of CaMKIIδ/MEF2 in Hypertensive and Physiological Cardiac Hypertrophy
Authors: Zhu Man , Shi Lijun 
Published: October 2018

Objective The type II calcium/calmodulin-dependent protein kinase IIδ (CaMKIIδ) signal plays a key role in the development of cardiac hypertrophy. This study used CaMKIIδ as an entry point to investigate the mechanism of moderate-intensity aerobic exercise affecting myocardial function.
Methods Male spontaneously hypertensive rats (SHRs) and Wistar-Kyoto rats (WKYs), 12 weeks age, were randomly divided into aerobic exercise group (SHR-EX/WKY-EX) and sedentary control group (SHR-SED/WKY-SED), with 12 rats in each group. The aerobic exercise group conducted an 8-week treadmill exercise training with a slope of 0°, 20m/min (about 55-65% of maximal aerobic velocity), 60min/day, and 5d/wk. The control group did not exercise. The body weight of each group of rats was measured weekly and the blood pressure of the rats was measured non-invasively. After 8 weeks, the hearts of SHR-EX group, WKY-EX group, SHR-SED group and WKY-SED group were weighed, and then myocardial tissue sections were taken for HE staining to observe the thickness of the ventricular wall and the morphology of myocardial cells. The expression of CaMKIIδ and MEF2 in each group was determined by Western blotting.
Results (1) The body weight of SHR-SED group was significantly higher than that of SHR-EX group (p<0.01), and the heart weight of rats in exercise group changed significantly. The WKY-EX group had greater heart weight than the WKY-SED group, and the SHR-SED group was heavier than the SHR-EX group (p<0.05). The heart weight/body weight ratio of the WKY-EX group was significantly higher than that of the WKY-SED group (p<0.01). The heart weight/body weight ratio of SHR-EX group and SHR-SED group was higher than that of WKY-EX group and WKY-SED group (p<0.01). (2) Compared with the WKY-SED group, the SHR-SED group had loose interstitial cells and increased single cell area. The SHR-EX group is more compact than the SHR-SED group, and the cell cross-sectional area is reduced. (3) The expression of CaMKIIδ protein in SHR-EX group was significantly lower than that in SHR-SED group (p<0.01), but the expression level of CaMKIIδ in WKY-EX group was significantly higher than that in WKY-SED group (p<0.01). The expression level of CaMKIIδ was significantly higher in the SHR-SED group than in the WKY-SED group. In addition, the expression of MEF2 protein in SHR-EX group and WKY-SED group was significantly lower than that in SHR-SED group (p<0.01), while the MEF2 expression level in WKY-EX group was higher than WKY-SED group and SHR-EX group (p<0.05).
Conclusions There is an interaction between aerobic exercise and hypertension. Aerobic exercise can effectively delay the development of hypertensive cardiac hypertrophy by regulating the expression of CaMKIIδ and MEF2 protein in the myocardium, but it can also cause cardiac hypertrophy in normal heart. It is one of the important mechanisms affecting the myocardial morphology and function.
PO-156 Effects of acute hypoxic exercise on TRPV4 channels in prefrontal cortex of rats
Authors: Huang Xing , Hu Yang 
Published: October 2018

Objective At the early stage of high altitude training, the decrease of motor ability caused by hypoxia makes it impossible for the training to be carried out normally. Since 90s, researchers have focused more on the decrease of the cardiopulmonary function and muscle oxygen utilization capacity, but there are few studies on cerebral hemodynamic factors, and no related mechanism studies. This study will take this as a starting point, to study the acute hypoxic exercise and its biological mechanism for brain hemodynamic effects of prefrontal cortex, providing a new target in order to improve the athletes at the beginning of the plateau duration and decreased exercise capacity problems.
Methods In order to explore the changes of the TRPV4 channels protein in rat prefrontal cortex induced by exercise under hypoxic exposure, this study used HE staining and immunohistochemical method to analyze the pathological changes and TRPV4 channel protein expression of prefrontal cortex in rats during incremental exercise in different oxygen concentration environment.
Results The results showed that cortex lesions characterized by venous congestion and neuronophagia, when exercise to level 5 under the condition of the simulated 4500 meters altitude, which was similar to normoxia exercise to level 8. With the increasing of exercise load, the expressions of TRPV4 in rat prefrontal cortex were significantly increased. And the TRPV4 expressions of every groups in the simulated 4500 meters altitude were significantly higher than those in normoxia groups.
Conclusions The results indicate that increased load exercise in acute hypoxia can lead to pathological changes of brain tissue much earlier that in normoxia condition, and increase the expression of TRPV4 channel protein in brain tissue. This study will provide valuable data for the study of the role of Ca2+ channels in the membrane of central nervous system in the phenomenon of the centers fatigue appearing earlier in hypoxic exercise.
PO-163 Aerobic exercise activates myocardial FGF21/FGFR1/PI3K-AKT signaling pathway and inhibits cardiomyocyte apoptosis in post-myocardial infarction rats
Authors: Li Mingxiao , Tian Zhenjun 
Published: October 2018

Objective To investigate the effect of aerobic exercise on the expression of fibroblast growth factor 21 (FGF21) and cardiomyocyte apoptosis in Myocardial Infarction (MI) rats.
Methods male SD rats were randomly divided into three groups：the sham operation (S), sedentary MI group (MI) and MI with aerobic exercise group (ME). The MI model was established by ligation of the left anterior descending branch of the left coronary artery. ME group were trained four weeks after the operation. LVSP, LVEDP and ±dp/dtmax were used to evaluate cardiac function. H9C2 cardiomyocytes were stimulated by 400 μmol/L H2O2 for 4h to simulate myocardial apoptosis mode. AMPK agonist AICAR and FGF21 receptor inhibitor PD166866 were used to interfere with H9C2. Myocardial collagen volume fraction was calculated by Masson staining and myocardium FGF21, FGFR1, Bax, Bcl-2 and PI3K-AKT pathway by western blotting or RT-Qpcr. Cardiomyocytes apoptosis was evaluated by TUNEL.
Results Compared with S, the expression of FGF21, FGFR1, Bax, Bcl-2 and PI3K, AKT increased significantly in MI group, the apoptotic cardiomyocytes and collagen fibers increased significantly, but the cardiac function decreased. Compared to MI, myocardium FGF21, FGFR1 and PI3K, AKT were further increased in ME group, the Bax/Bcl-2 and the apoptotic cardiomyocytes decreased significantly. The percentage of collagen fibers decreased and the cardiac function was improved. Myocardium FGF21 was positively correlated with the inhibition of cardiomyocyte apoptosis and the improvement of cardiac function. Furthermore, the expression of Bax/Bcl-2, TNF-α/IL-10 and the apoptotic cardiomyocytes was significantly increased by PD166866, but PI3K-AKT pathway decreased significantly by PD166866. However, AICAR single intervention or PD166866 simultaneous intervention also can reverse this adverse effects.
Conclusions Exercise can increase myocardial FGF21/FGFR1 with MI. The one of the mechanisms is to activate PI3K-AKT pathway to inhibit cardiaomyocyte apoptosis and inflammatory. It indicates that FGF21/FGFR1/PI3K-AKT signaling pathway plays an important role in inhibiting myocardial apoptosis and improving cardiac function.
PO-167 Effects of Hydrogen-rich Water on Rat Skeletal Muscles' Oxidative Damage and Autophagy Induced by Repeated Exhaustive Exercise
Authors: Xu Zujie , Gu Hongdi , Tian Zhenjun 
Published: October 2018

Objective This study was carried out to detect the effects of hydrogen-water intervention on oxidative stress and cell autophagy in skeletal muscle of rats with repeated exhaustion.
Methods 30 male SD rats in the age of 3 months, weighing 180-210g, were randomly divided into control group (C), repeated exhaustion group (EX), and the repeated exhaustion with hydrogen intervention group (EH), 10 rats in each group. The EX and EH groups were subjected to a four-weeks of repeated-exhaustive exercise. The initial speed of the exercise was 15 m/min, and increasedby 5 m/min every 5 min, the final speed is 35m/min until exhaustion, 5 d/wk, with a total of 4 wk. In EH group, hydro-water was given to rats 30 mins before exercise. The ultrastructural changes of skeletal muscle were observed by using a transmission electron microscopy. Activity of SDH and CK in serum and SOD activity, MDA content and T-AOC level in skeletal muscle tissue were detected. Western blotting was used to detect the proteins expression of autophagy related proteins in skeletal muscle, mTOR, p-mTOR, LC3B-2 and P53.
Results Compared with the EX group, in the EH group, the ultrastructural damage and mitochondrial swelling were significantly reduced, and the time of exhaustion was significantly prolonged (p<0.05), Serum SDH activity increased significantly (p<0.05), CK activity decreased significantly (p<0.05), and skeletal muscle tissue SOD activity and total antioxidant capacity significantly increased (p<0.05), MDA content decreased significantly (p<0.01), mTOR and p-mTOR protein expression was significantly increased(p<0.05), the LC3B-2 and P53 protein expression was significantly lower (p<0.05).
Conclusions Hydrogen water intervention could significantly improve repeatedly exhaustion exercise result in rat skeletal muscle injury, oxidative stress and cell ultrastructure damage excessive autophagy, improving oxidation resistance of the skeletal muscle and exercise endurance.