bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-Wide Analysis of Glucocorticoid-Responsive Transcripts in the Hypothalamic Paraventricular Region of Male Rats
Authors: Itoi Keiichi , Motoike Ikuko , Liu Ying , Clokie Sam , Iwasaki Yasumasa , Uchida Katsuya , Sato Tatsuya , Aguilera Greti 
Published: October 2018

Abstract
Glucocorticoids (GCs) are essential for stress adaptation, acting centrally and in the periphery. Corticotropin-releasing factor (CRF), a major regulator of adrenal GC synthesis, is produced in the paraventricular nucleus of the hypothalamus (PVH), which contains multiple neuroendocrine and preautonomic neurons. GCs may be involved in diverse regulatory mechanisms in the PVH, but the target genes of GCs are largely unexplored except for the CRF gene (Crh), a well-known target for GC negative feedback. Using a genome-wide RNA-sequencing analysis, we identified transcripts that changed in response to either high-dose corticosterone (Cort) exposure for 12 days (12-day high Cort), corticoid deprivation for 7 days (7-day ADX), or acute Cort administration. Among others, canonical GC target genes were upregulated prominently by 12-day high Cort. Crh was upregulated or downregulated most prominently by either 7-day ADX or 12-day high Cort, emphasizing the recognized feedback effects of GC on the hypothalamic-pituitary-adrenal (HPA) axis. Concomitant changes in vasopressin and apelin receptor gene expression are likely to contribute to HPA repression. In keeping with the pleotropic cellular actions of GCs, 7-day ADX downregulated numerous genes of a broad functional spectrum. The transcriptome response signature differed markedly between acute Cort injection and 12-day high Cort. Remarkably, six immediate early genes were upregulated 1 hour after Cort injection, which was confirmed by quantitative reverse transcription PCR and semiquantitative in situ hybridization. This study may provide a useful database for studying the regulatory mechanisms of GC-dependent gene expression and repression in the PVH.
Hypoxia-ischemia alters distribution of lysosomal proteins in rat cortex and hippocampus
Authors: Troncoso M. , Bannoud N. , Carvelli L. , Asensio J. , Seltzer A. , Sosa M. A. 
Published: October 2018

ABSTRACTNeuronal excitotoxicity induced by glutamatergic receptor overstimulation contributes to brain damage. Recent studies have shown that lysosomal membrane permeabilization (LMP) is involved in ischemia-associated neuronal death. In this study we evaluated the effect of neonatal hypoxia-ischemia (HI), as a model of excitotoxicity, on the lysosomal integrity throughout the distribution of the lysosomal proteins cathepsin D and prosaposin. Rat pups (7 days old) of the Wistar Kyoto strain were submitted to HI and they were euthanized 4 days after treatment and the cerebral cortex (Cx) and hippocampus (HIP) were processed for immunohistochemistry or immunoblotting. Treatment induced an increase of gliosis and also a redistribution of both prosaposin and cathepsin D (as intermediate and mature forms), into the cytosol of the HIP and Cx. In addition, HI induced a decrease of LAMP-1 in the membranous fraction and the appearance of a reactive band to anti-LAMP-1 in the cytosolic fraction, suggesting a cleavage of this protein. From these results, we propose that the abnormal release of Cat D and PSAP to the cytosol is triggered as a result of LAMP-1 cleavage in HI animals, which leads to cell damage. This could be a common mechanism in pathological conditions that compromises neuronal survival and brain function.
Zebrafish knock-ins swim into the mainstream
Authors: Prykhozhij Sergey V. , Berman Jason N. 
Published: October 2018

ABSTRACT
The zebrafish is an increasingly popular model organism for human genetic disease research. CRISPR/Cas9-based approaches are currently used for multiple gene-editing purposes in zebrafish, but few studies have developed reliable ways to introduce precise mutations. Point mutation knock-in using CRISPR/Cas9 and single-stranded oligodeoxynucleotides (ssODNs) is currently the most promising technology for this purpose. Despite some progress in applying this technique to zebrafish, there is still a great need for improvements in terms of its efficiency, optimal design of sgRNA and ssODNs and broader applicability. The papers discussed in this Editorial provide excellent case studies on identifying problems inherent in the mutation knock-in technique, quantifying these issues and proposing strategies to overcome them. These reports also illustrate how the procedures for introducing specific mutations can be straightforward, such that ssODNs with only the target mutation are sufficient for generating the intended knock-in animals. Two of the studies also develop interesting point mutant knock-in models for cardiac diseases, validating the translational relevance of generating knock-in mutations and opening the door to many possibilities for their further study.
Factors affecting length of stay after percutaneous biliary interventions
Authors: Roy Mayank , Kyaw Tun Jimmy , Banerjee Abhirup , Mohandas Shailesh , Abraham Ajit T , Hutchins Robert R , Bhattacharya Satyajit , Renfrew Ian , Low Deborah , Fotheringham Tim , Kocher Hemant M 
Published: October 2018

Objective:
To evaluate the factors affecting the length of hospital stay (LOS) after percutaneous transhepatic biliary drainage (PTBD).

Methods:
A retrospective review of all patients who had undergone PTBD with or without stenting at a UK specialist centre between 2005 and 2016 was conducted.

Results:
692 patients underwent 1976 procedures over 731 clinical episodes for which, the median age was 65 (range 18–100) years, and the median Charlson Index was 3. PTBD was performed for malignant (n = 563) and benign strictures (n = 60), stones (n = 62), and bile leaks (n = 46). The median LOS was 13 (range 0–157) days, and the median interprocedure duration was 9 (range 0–304) days. The median number of procedures per patient was 2 and the median number of days required to complete a set of procedures for a patient (TBID) ranged from 0 to 557 days, with a median of 16 (interquartile range: 8–32) days. Patients with biliary leak had the highest LOS. Biliary stents were mostly placed at the second stage at a median of 6 (range 0–120) days from the first procedure day. Placement of a biliary stent in the first stage of the procedure was associated with shorter LOS (p &lt; 0.001).

Conclusions:
Biliary stenting at index procedure reduces LOS, although it is not always technically possible. Patients with bile leak managed with PTBD have longer LOS.

Advances in knowledge:
This study provides data which can help in appropriate consenting, better planning, and efficient resource utilization for patients undergoing PTBD.
Model of entrepreneurship competence as a basis for the development of entrepreneurship education
Authors: Venesaar Urve , Täks Marge , Arro Grete , Malleus Elina , Loogma Krista , Mädamürk Kaja , Titov Eneken , Toding Martin 
Published: October 2018

Artikli eesmärk on kirjeldada ja empiiriliselt põhjendada ettevõtluspädevuse mudelis sisalduvate alapädevuste valikut, et luua alus ettevõtlusõppe arendamiseks ning toetada õppijate ettevõtluspädevuse arengut ja toimetulekut nii töös (sh ettevõtluses) kui ka igapäevaelus. Ettevõtluspädevuse mudel hõlmab 14 alapädevust, mis on jaotatud enesejuhtimise, väärtust loova mõtlemise, sotsiaalsete olukordade lahendamise ja äriideede elluviimise valdkonnaks. Kõrgkooliõpilaste (N = 1479) hulgas tehtud uuringust ilmneb, et seosed alapädevuste vahel on ootuspärased ja vastavad teoreetilistele eeldustele. See lubab arvata, et alapädevused moodustavad terviku ning on usaldusväärseks aluseks õppija ettevõtluspädevuse arendamisele ja toetamisele. Artikkel soodustab diskussiooni ettevõtluspädevuse ja selle arendamise võimaluste üle, pakkudes ühtlasi välja lähtekoha ettevõtlusõppe arendamiseks Eesti haridussüsteemis.
Summary
Speech and the dental interface
Authors: Hyde Amy C , Moriarty Lynne , Morgan Annie G , Elsharkasi Lamis M , Deery Chris 
Published: October 2018

This article outlines how sounds are produced and how speech and language develop, in the child. The assessment of speech by a speech and language therapist is briefly described. It then discusses the evidence for an impact of occlusion, and the loss or absence of teeth on speech. In summary, there is a possibility that the loss, absence or malalignment of teeth may affect speech, but unfortunately the evidence base is weak.CPD/Clinical Relevance: Dental care professionals should understand the development of speech and how dental health potentially affects speech.
Primary failure of eruption or mechanical failure of eruption?
Authors: Sharma Geetanjali , Kneafsey Louise , Ashley Paul , Noar Joseph 
Published: October 2018

Failure of eruption (FOE) of first and second permanent molars is rare, with an estimated prevalence of 0.01% in the case of the first permanent molar and 0.06% in the case of the second. 1 When there is failure of eruption of a permanent molar, the following differential diagnoses should be considered in addition to tooth impaction: primary failure of eruption (PFE) (Type I/Type II) and mechanical failure of eruption (MFE) owing to ankylosis. Limited studies have proposed protocols for the management of failure of eruption (FOE) of permanent molars with no clear consensus or guidelines. CPD/Clinical Relevance: Making an early distinction between MFE and PFE is important in the management of patients with failure of eruption of permanent molars since they dictate different treatment modalities. This paper reviews the clinical characteristics of FOE of permanent molars to aid the clinician in diagnosis and subsequent management of this rare and challenging condition.
Clinical Pharmacogenetics for Precision Medicine: Successes and Setbacks
Authors: Chambliss Allison B , Marzinke Mark A 
Published: October 2018

Abstract

Background
Pharmacogenetics is a key component in the delivery of therapeutics to maximize pharmacologic efficacy and minimize toxicity. There are numerous identified gene–drug pairs that demonstrate the utility of pharmacogenetics testing for drug or dose selection. Although some of these pairs have translated into clinical use, pharmacogenetic testing has not yet made its way into routine clinical practice at many institutions.

Content
This review provides an overview of clinically actionable pharmacogenetics in precision medicine. Examples of successfully implemented gene–drug pairs, along with common testing methodologies and guidelines for application, are discussed. Remaining barriers to widespread clinical implementation are also examined.

Summary
There is a recognized role for genotyping in the guidance of therapeutic drug regimens and the prevention of adverse drug reactions. Evidence-based guidelines are available to aid in the selection of treatment upon pharmacogenetics testing for established gene–drug pairs. Multidisciplinary clinical collaboration and clinical decision support tools will be critical for widespread adoption, and financial reimbursement barriers remain.
Predicting consumer intention to purchase clothing products made from sustainable fabrics
Authors: Yoo Jeong-Ju , Divita Lorynn , Kim Hye-Young 
Published: October 2018

Abstract
The goal of this study is to investigate the differential influences of environmental concern and fashion opinion leadership on consumer intention to purchase clothing products made of sustainable fabrics. In doing so, this study examines the mediating role of environmental attitude towards clothing consumption. The data were collected from 122 US college students with an average age of 21.4. The majority of the participants were female (83.6 per cent) and Caucasian (64.5 per cent). A weighted least squares hierarchical regression analysis was used for the data analysis. The results suggested that fashion opinion leadership was a more powerful determinant than environmental concern in predicting consumer intention to purchase clothing products made of sustainable fabrics. However, environmental attitude towards clothing consumption did not serve as a significant mediator. These findings provide beneficial insight for fast fashion retailers mainly targeting fashion opinion leaders. Managerial implications are discussed to delineate how fast fashion retailers can benefit from sustainable production and retailing strategies.
Notes on wearer–worn attachments: Learning to wear
Authors: Valle-Noronha Julia , Niinimäki Kirsi , Kujala Sari 
Published: October 2018

Abstract
Previous literature in person–product attachment has identified factors in long-term relationships responsible for the strengthening of bonds between users and products, stimulating longevity in use. Interested in further understanding the matter in the realm of fashion, this study investigates how relationships between individuals and the clothes they wear evolve over time. It identifies motivators behind the increase and decrease in the overall quality of wearer–worn relationships in regard to four dimensions: comfort, frequency of use, visuality and versatility. In order to achieve this aim, an adaptation of the UX curve method is used. The method was employed with a group of ten participants, wearers of specific clothing production, namely experimental fashion, in contrast with commercial fashion pieces. The study findings contribute to the literature on person–product attachment and highlight ‘learning to wear’ as an engaging experience encouraging stronger relations with clothes. In the discussion, the article proposes future endeavours to understand wearing practices aiming at more engaging designs.
Oxidative stress in mollusks Biomphalaria glabrata exposed to gamma radiation
Authors: Silva Luanna Ribeiro Santos , Augusto Ricielle Lopes , Siqueira Williams Nascimento de , Pereira Dewson Rocha , Silva Edvane Borges da , Melo Ana Maria Mendonça de Albuquerque 
Published: October 2018

Ionizing radiation can cause biological changes in different organisms such as mollusks from Biomphalaria glabrata species, in which alterations could be observed in the reproductive system of the specimens, prejudicing fertility and fecundity. As the changes may occur due to the lipid peroxidation caused by the action of free radicals on the gonads, the objective of this work was to evaluate the oxidative damage caused by the exposure of B. glabrata mollusks to different doses of 60Co gamma radiation. In addition, efforts were carried out to standardize a sensitive and low-cost technique for detecting negative effects caused by high doses of ionizing radiation. For this, each mollusk group (n = 10) was submitted to 0 (control), 10, 15, 20 and 25 Gy (gammacell 60Co, dose rate 3.53 kGy/h). The TBARS method was applied for the quantification of lipid peroxidation of the gonads of the mollusks after 24 and 48 h. ANOVA, followed by the mean comparison (Tukey) at the 5% of significance level (p<0.05), indicated high concentrations of TBARS in the gonads after 24 h. Otherwise, after 48 h, differences for TBARS concentrations were not significant, determining that the action of free radicals from ionizing radiation on cell membranes mainly occurred within 24 h after irradiation. Therefore, the TBARS assay could be applied for detecting oxidative stress caused by short exposure of B. glabrata to ionizing radiation.
Sprint-Specific Training in Youth: Backward Running vs. Forward Running Training on Speed and Power Measures in Adolescent Male Athletes
Authors: Uthoff Aaron , Oliver Jon , Cronin John , Harrison Craig , Winwood Paul 
Published: October 2018

Abstract
Uthoff, A, Oliver, J, Cronin, J, Harrison, C, and Winwood, P. Sprint-specific training in youth: Backward running vs. forward running training on speed and power measures in adolescent male athletes. J Strength Cond Res 34(4): 1113–1122, 2020—This study compared the effects of 2 sprint-specific training programs against the natural development of speed, power, and stiffness in a group of adolescent male athletes. Forty-three male adolescents (aged 13–15 years) were randomly assigned to 1 of 2 training groups; backward running training (BRT = 26), or forward running training (FRT = 17). A physical education class (n = 24) of similar age constituted a control (CON) group. Both training groups performed running sessions matched for distance and intensity biweekly for 8 weeks. Parametric and magnitude-based inferences were used to analyze within group (pre-post measures) and between group (gain scores) for 10-m, 10- to 20-m, and 20-m sprint times, vertical countermovement jump (CMJ) height, and vertical leg stiffness. Both running groups significantly improved (p ≤ 0.05) in all performance tests from pre-training to post-training, with effect sizes ranging from −1.25 to 0.63. When the groups were compared, the BRT and FRT groups improved significantly (p ≤ 0.01) on all sprint performances and stiffness relative to the CON group. The BRT group demonstrated favorable effects for 10-m and 20-m sprint performances (effect size [ES] = −0.47 and −0.26, respectively) and CMJ height (ES = 0.51) compared with the FRT group. These results demonstrate that forward and backward sprint-specific training programs enhance speed and power measures more than natural development in adolescent male athletes. Furthermore, the greater training responses in sprint performance and CMJ ability indicate that BRT is a useful tool for improving concentric strength and power and may be classified as a sprint-specific training method.
Diş Hekimliği Fakültesi’ne başvuran hastaların dental implant farkındalıklarının değerlendirilmesi
Authors: Ünal Erzurumlu Zerrin , Kara Zeliha Sertap 
Published: October 2018

Amaç: Bu çalışmada T.C. Ordu Üniversitesi Diş Hekimliği Fakültesi Ağız,
Diş ve Çene Radyolojisi Anablim Dalı’na başvuran hastaların dental implant
hakkında farkındalıklarının değerlendirilmesi amaçlanmıştır.Gereç ve Yöntemler: Çalışma 18 yaşveüzeri 387 (227 kadın, 160 erkek) hastanın kendi
isteğiyle ankete katılımı ile gerçekleştirilmiştir. Demografik yapıyı da içeren
çoktan seçmeli standardize edilmiş anket formu hastalar tarafından
doldurulmuştur. Sonuçların istatistiksel değerlendirmesi frekans dağılımı ve
ki-kare testi kullanılarak yapılmıştır.Bulgular: Katılımcıların %66.7'sinde(258)
dental implant farkındalığının bulunduğu
tespit edilmiştir. Çalışmamızda dental implant farkındalığının eğitim durumu
ile ilişkisi istatistiksel olarak anlamlı bulunmuştur (p&lt;0.05). Dental implant farkındalığı olan
katılımcıların %51.6'sının bilgi kaynağının “dişhekimi” olduğu belirlenmiştir.
Tüm katılımcıların %86.6'sı implant
hakkındaki bilgileri dişhekimlerinden öğrenmek istediğini bildirmiştir.
Farkındalığı olan katılımcıların, %56.2'si
implant tedavisini isterken %6.6'sı
implant tedavisini istemediğini ve %37.2'si
kararsız olduğunu bildirmiştir.Sonuçlar: Çalışmamızın
sınırları dahilinde, implant tedavisi konusunda bireylerin kararsız olma
nedeninin bu konuda yeterli bilgiye sahip olmamalarından kaynaklandığı tespit
edilmiştir. Bu nedenle hastaların implant hakkında farkındalıklarının
arttırılabilmesi için eğitim programları düzenlenmelidir.

Anahtar kelimeler:Dental
implant, Eksik diş, Farkındalık, Anket
MARCHA DE ABSORÇÃO DE MICRONUTRIENTES EM PLANTAS DE PIMENTÃO SOB FERTIRRIGAÇÃO EM AMBIENTE PROTEGIDO
Authors: Marcussi Francisco Fernando Noronha , Bôas Roberto Lyra Villas 
Published: October 2018

MARCHA DE ABSORÇÃO DE MICRONUTRIENTES EM PLANTAS DE PIMENTÃO SOB FERTIRRIGAÇÃO EM AMBIENTE PROTEGIDO Francisco Fernando Noronha MarcussiDepartamento de Engenharia Rural, Faculdade de Ciências Agronômicas,Universidade Estadual Paulista, Botucatu, SP. CP 237, CEP 18603-970. E-mail: fmarcussi@hotmail.comRoberto Lyra Villas BôasDepartamento de Recursos Naturais e Ciência do Solo, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, Botucatu, SP CP 237, CEP 18603-970. E-mail:rlvboas@fca.unesp.br 1 RESUMO Esta pesquisa teve por objetivo determinar a marcha de absorção de micronutrientes da planta de pimentão (híbrido Elisa), sob fertirrigação, através de oito épocas de coleta de planta. Ao final do experimento foi determinada a quantidade de micronutrientes absorvida pela planta, bem como a necessidade micro nutricional da planta em cada etapa de crescimento. O experimento foi desenvolvido em área do Departamento de Recursos Naturais - Ciência do Solo da Faculdade de Ciências Agronômicas, campus de Botucatu. Os tratamentos constaram de oito épocas de coleta de planta (0, 20, 40, 60, 80, 100, 120, e 140 dias após o transplante das mudas) e quatro repetições, sendo cada repetição formada por quatro plantas. A fertirrigação foi via gotejamento. Os resultados de extração de micronutrientes demonstraram que o maior desenvolvimento da planta, conseqüentemente, sua maior necessidade nutricional de B, Cu, Fe, Mn e Zn, se concentra no período entre 120 e 140 dias após o transplante de mudas, onde verificou-se que a planta dobrou a extração total dos micronutrientes analisados em comparação ao período anterior. UNITERMOS: fertirrigação, pimentão, Capsicum annuum L., irrigação por gotejamento e acúmulo de micronutrientes MARCUSSI, F.F.N.; VILLAS BÔAS, R.L. ABSORPTION COURSE OF MICRONUTRIENTS IN BELL PEPPER PLANT UNDER FERTIGATION IN PROTECTED ENVIRONMENT 2 ABSTRACT This research aimed to determine the absorption course of micronutrients in bell pepper plants (Elisa hybrid) under fertigation using eight time periods of plant collection. At the end of the experiment the amount of absorbed and accumulated micronutrients by plants has been determined as well as the plant nutritional needs in each growth stage. The experiment was carried out at the Natural Resource Department – Soil Science at the Agricultural Science College, Botucatu campus, São Paulo state, Brazil. The treatments were eight time periods of plant collection (0, 20, 40, 60, 80, 100, 120, and 140 days after seedling transplant) and four repetitions, each one comprising four plants. The fertigation was performed through drip irrigation. The largest plant development and consequently the highest B, Cu, Fe, Mn and Zn nutritional needs occurred from 120 to 140 days after seedling transplant where the plant doubled the whole analyzed micronutrient extraction compared to the earlier period. KEYWORDS: fertigation, bell pepper, Capsicum annuum L., drip irrigation and micronutrient absorption.