bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New Frozen Soil Parameterization Including Frost and Thaw Fronts in the Community Land Model
Authors: Gao Junqiang , Xie Zhenghui , Wang Aiwen , Liu Shuang , Zeng Yujin , Liu Bin , Li Ruichao , Jia Binghao , Qin Peihua , Xie Jinbo 
Published: February 2019

AbstractFreeze–thaw processes in soils, including changes in frost and thaw fronts (FTFs), are very sensitive to warming. However, the latest climate models do not predict changes in FTFs directly. In this study, a new frozen soil parameterization including changes in FTFs was incorporated into the Community Land Model version 4.5 for climate modeling, which we denote CLM4.5_FTF. A set of numerical experiments including single points, regions in China, and a global scale were conducted using the model to validate its performance. The simulated FTF depths compare well with observed data from both the D66 station (permafrost) and Hulugou station (seasonally frozen ground). The simulated active layer thickness, defined as the maximum thaw front depth in permafrost, is in general agreement but slightly greater than observations from the Circumpolar Active Layer Monitoring program. The simulated distributions of different types of frozen soil in China and permafrost in the northern hemisphere are in agreement with the frozen soil map of China and the International Permafrost Association map, respectively. The results confirm that the model performs well for FTF simulations. The model was also used for year‐long simulations of soil temperature and freeze–thaw processes to check its applicability in continuous simulation. The results show that CLM4.5_FTF performed better than the original model, and the improvement was better for lower levels than for the upper level. Finally, we give simulated latent heat flux, sensible heat flux, and 10‐cm soil temperature deviations determined via the couple model with and without the new scheme.
P2Y12 regulates microglia activation and excitatory synaptic transmission in spinal lamina II neurons during neuropathic pain in rodents
Authors: Yu Tingting , Zhang Xin , Shi Haosong , Tian Jinge , Sun Lingling , Hu Xueming , Cui Wenqiang , Du Dongping 
Published: February 2019

AbstractPeripheral nerve injury causes neuropathic pain and microglia activation. P2Y12 receptors on microglia are thought to be a key player in the surveillance of the local environment, but whether or how these receptors are engaged in the cross-talk between microglia and neurons of the dorsal horn remain ambiguous. Using a rodent model of nerve injury-induced pain, we investigated the roles of P2Y12 in microglia activation, excitatory synaptic transmission, and nociceptive allodynia. We found that spinal nerve ligation (SNL) significantly increased the level of P2Y12 receptors specifically in the microglia of the ipsilateral dorsal horn. Injections of P2Y12 antagonists (MRS2395 or clopidogrel) attenuated microglia activation and increased the paw withdrawal latency in response to thermal stimuli on the ipsilateral side without affecting the basal threshold on the contralateral side. These effects on pain behaviors were replicated in P2Y12 knockout mice. Patch-clamp recordings further revealed that partial sciatic nerve ligation (PSNL)-induced excessive miniature excitatory postsynaptic currents (mEPSCs) were significantly attenuated in P2Y12 knockout mice. Moreover, we found that SNL activates the GTP-RhoA/ROCK2 signaling pathway and elevates the level of phosphorylated p38 mitogen-activated protein kinase (MAPK), which was inhibited by the P2Y12 antagonist. The phosphorylation of p38 MAPK was inhibited by a ROCK inhibitor, but not vice versa, suggesting that p38 MAPK is downstream of ROCK activation. Our findings suggest that nerve injury engages the P2Y12 receptor-dependent GTP-RhoA/ROCK2 signaling pathway to upregulate excitatory synaptic transmission in the dorsal horn. This cross-talk ultimately participates in the manifestation of nociceptive allodynia, implicating P2Y12 receptor as a potential target for alleviating neuropathic pain.
Universal hidden order in amorphous cellular geometries
Authors: Klatt Michael A. , Lovrić Jakov , Chen Duyu , Kapfer Sebastian C. , Schaller Fabian M. , Schönhöfer Philipp W. A. , Gardiner Bruce S. , Smith Ana-Sunčana , Schröder-Turk Gerd E. , Torquato Salvatore 
Published: February 2019

AbstractPartitioning space into cells with certain extreme geometrical properties is a central problem in many fields of science and technology. Here we investigate the Quantizer problem, defined as the optimisation of the moment of inertia of Voronoi cells, i.e., similarly-sized ‘sphere-like’ polyhedra that tile space are preferred. We employ Lloyd’s centroidal Voronoi diagram algorithm to solve this problem and find that it converges to disordered states associated with deep local minima. These states are universal in the sense that their structure factors are characterised by a complete independence of a wide class of initial conditions they evolved from. They moreover exhibit an anomalous suppression of long-wavelength density fluctuations and quickly become effectively hyperuniform. Our findings warrant the search for novel amorphous hyperuniform phases and cellular materials with unique physical properties.
Articular cartilage and sternal fibrocartilage respond differently to extended microgravity
Authors: Fitzgerald Jamie , Endicott Jamie , Hansen Uwe , Janowitz Cathleen 
Published: February 2019

AbstractThe effects of spaceflight on cartilaginous structure are largely unknown. To address this deficiency, articular cartilage (AC) and sternal cartilage (SC) from mice exposed to 30 days of microgravity on the BION-M1 craft were investigated for pathological changes. The flight AC showed some evidence of degradation at the tissue level with loss of proteoglycan staining and a reduction in mRNA expression of mechano-responsive and structural cartilage matrix proteins compared to non-flight controls. These data suggest that degradative changes are underway in the AC extracellular matrix exposed to microgravity. In contrast, there was no evidence of cartilage breakdown in SC flight samples and the gene expression profile was distinct from that of AC with a reduction in metalloproteinase gene transcription. Since the two cartilages respond differently to microgravity we propose that each is tuned to the biomechanical environments in which they are normally maintained. That is, the differences between magnitude of normal terrestrial loading and the unloading of microgravity dictates the tissue response. Weight-bearing articular cartilage, but not minimally loaded sternal fibrocartilage, is negatively affected by the unloading of microgravity. We speculate that the maintenance of physiological loading on AC during spaceflight will minimize AC damage.
The role of oral magnesium supplements for the management of stable bronchial asthma: a systematic review and meta-analysis
Authors: Abuabat Faisal , AlAlwan Abdulaziz , Masuadi Emad , Murad Mohammad Hassan , Jahdali Hamdan Al , Ferwana Mazen Saleh 
Published: February 2019

AbstractAsthma is a chronic lung disease characterized by airway inflammation and hyper-responsiveness of airway smooth muscles. There is growing evidence that magnesium may have a role in managing asthma through its dual effect as an anti-inflammatory and bronchodilating agent. To assess the efficacy of oral magnesium supplements in chronic asthmatic patients. In addition to searching through Clinicaltrials.gov/ and references for oral magnesium supplement studies, we performed a database search in Medline, CINAHL, CENTRAL, and Embase. We contacted the authors of the included trials to ask for additional information. We included randomized controlled trials that compared oral magnesium supplements versus placebo, in addition to standard asthma treatment in mild-moderate asthmatic adults and children (older than 6 years). Two reviewers independently performed the study selection, data abstraction, and the assessment of the risk of bias. Eight trials at moderate risk of bias enrolling a total of 917 patients were included. Oral magnesium improved FEV1 at week 8 (5.69 (L/min); 95% CI: 1.92, 9.46; I2: 45%). There was no significant improvement in FEV1 at other follow up periods. There was no significant change in FVC, Methacholine challenge test, the frequency of bronchodilator use, or symptoms score. There were no data on mortality or quality of life. Oral magnesium supplements may lead to improvement in FEV1 that was only demonstrated at eight weeks; but no effect on any other outcome. Until future evidence emerges, oral magnesium cannot be recommended as adjuvants to standard treatment for mild to moderate asthmatic individuals.
Plague vaccine: recent progress and prospects
Authors: Sun Wei , Singh Amit K. 
Published: February 2019

AbstractThree great plague pandemics, resulting in nearly 200 million deaths in human history and usage as a biowarfare agent, have made Yersinia pestis as one of the most virulent human pathogens. In late 2017, a large plague outbreak raged in Madagascar attracted extensive attention and caused regional panics. The evolution of local outbreaks into a pandemic is a concern of the Centers for Disease Control and Prevention (CDC) in plague endemic regions. Until now, no licensed plague vaccine is available. Prophylactic vaccination counteracting this disease is certainly a primary choice for its long-term prevention. In this review, we summarize the latest advances in research and development of plague vaccines.
Effects of light and darkness on pH regulation in three coral species exposed to seawater acidification
Authors: Venn A. A. , Tambutté E. , Caminiti-Segonds N. , Techer N. , Allemand D. , Tambutté S. 
Published: February 2019

AbstractThe resilience of corals to ocean acidification has been proposed to rely on regulation of extracellular calcifying medium pH (pHECM), but few studies have compared the capacity of coral species to control this parameter at elevated pCO2. Furthermore, exposure to light and darkness influences both pH regulation and calcification in corals, but little is known about its effect under conditions of seawater acidification. Here we investigated the effect of acidification in light and darkness on pHECM, calcifying cell intracellular pH (pHI), calcification, photosynthesis and respiration in three coral species:Stylophora pistillata,Pocillopora damicornisandAcropora hyacinthus. We show thatS. pistillatawas able to maintain pHECMunder acidification in light and darkness, but pHECMdecreased inP. damicornisandA. hyacinthusto a much greater extent in darkness than in the light. Acidification depressed calcifying cell pHIin all three species, but we identified an unexpected positive effect of light on pHI. Calcification rate and pHECMdecreased together under acidification, but there are inconsistencies in their relationship indicating that other physiological parameters are likely to shape how coral calcification responds to acidification. Overall our study reveals interspecies differences in coral regulation of pHECMand pHIwhen exposed to acidification, influenced by exposure to light and darkness.
The effect of metformin therapy on incidence and prognosis in prostate cancer: A systematic review and meta-analysis
Authors: He Kancheng , Hu Huating , Ye Senlin , Wang Haohui , Cui Rongrong , Yi Lu 
Published: February 2019

AbstractThe relationship between metformin and prostate cancer (PCa) remains controversial. To clarify this association, the PubMed, Embase and Cochrane library databases were systematically searched from their inception dates to May 23, 2018, using the keywords “metformin” and “prostate cancer” to identify the related studies. The results included incidence, overall survival (OS), PCa-specific survival (CSS) and recurrence-free survival (RFS), which were measured as hazard ratios (HR) with a 95% confidence interval (95% CI) using Review Manager 5.3 software. A total of 30 cohort studies, including 1,660,795 patients were included in this study. Our study revealed that metformin treatment improves OS, CSS and RFS in PCa (HR = 0.72, 95% CI: 0.59–0.88, P = 0.001; HR = 0.78, 95% CI: 0.64–0.94, P = 0.009; and HR = 0.60, 95% CI: 0.42–0.87 P = 0.006, respectively) compared with non-metformin treatment. However, metformin usage did not reduce the incidence of PCa (HR = 0.86, 95% CI: 0.55–1.34, P = 0.51). In conclusion, compared with non-metformin treatment, metformin therapy can significantly improve OS, CSS and RFS in PCa patients. No association was noted between metformin therapy and PCa incidence. This study indicates a useful direction for the clinical treatment of PCa.
Role of Cholecystectomy in Choledocholithiasis Patients Underwent Endoscopic Retrograde Cholangiopancreatography
Authors: Wang Chi-Chih , Tsai Ming-Chang , Wang Yao-Tung , Yang Tzu-Wei , Chen Hsuan-Yi , Sung Wen-Wei , Huang Shih-Ming , Tseng Ming-Hseng , Lin Chun-Che 
Published: February 2019

AbstractThere are no clinical guidelines for the timing of cholecystectomy (CCY) after performing therapeutic endoscopic retrograde cholangiopancreatography (ERCP) for choledocholithiasis. We tried to analyze the clinical practice patterns, medical expenses, and subsequent outcomes between the early CCY, delayed CCY, and no CCY groups of patients. 1827 choledocholithiasis patients who underwent therapeutic ERCP were selected from the nationwide population databases of two million random samples. These patients were further divided into early CCY, delayed CCY, and no CCY performed. In our analysis, 1440 (78.8%) of the 1827 patients did not undergo CCY within 60 days of therapeutic ERCP, and only 239 (13.1%) patients underwent CCY during their index admission. The proportion of laparoscopic CCY increased from 37.2% to 73.6% in the delayed CCY group. There were no significant differences (p = 0.934) between recurrent biliary event (RBE) rates with or without early CCY within 60 days of ERCP. RBE event-free survival rates were significantly different in the early CCY (85.04%), delayed CCY (89.54%), and no CCY (64.45%) groups within 360 days of ERCP. The method of delayed CCY can reduce subsequent RBEs and increase the proportion of laparoscopic CCY with similar medical expenses to early CCY in Taiwan’s general practice environment.
Harnessing neurovascular interaction to guide axon growth
Authors: Partyka Paul P. , Jin Ying , Bouyer Julien , DaSilva Angelica , Godsey George A. , Nagele Robert G. , Fischer Itzhak , Galie Peter A. 
Published: February 2019

AbstractRegulating the intrinsic interactions between blood vessels and nerve cells has the potential to enhance repair and regeneration of the central nervous system. Here, we evaluate the efficacy of aligned microvessels to induce and control directional axon growth from neural progenitor cells in vitro and host axons in a rat spinal cord injury model. Interstitial fluid flow aligned microvessels generated from co-cultures of cerebral-derived endothelial cells and pericytes in a three-dimensional scaffold. The endothelial barrier function was evaluated by immunostaining for tight junction proteins and quantifying the permeability coefficient (~10−7 cm/s). Addition of neural progenitor cells to the co-culture resulted in the extension of Tuj-positive axons in the direction of the microvessels. To validate these findings in vivo, scaffolds were transplanted into an acute spinal cord hemisection injury with microvessels aligned with the rostral-caudal direction. At three weeks post-surgery, sagittal sections indicated close alignment between the host axons and the transplanted microvessels. Overall, this work demonstrates the efficacy of exploiting neurovascular interaction to direct axon growth in the injured spinal cord and the potential to use this strategy to facilitate central nervous system regeneration.
Increase in Interfacial Adhesion and Electrochemical Charge Storage Capacity of Polypyrrole on Au Electrodes Using Polyethyleneimine
Authors: Kim Kyung-Geun , Kim Sung Yeol 
Published: February 2019

AbstractHigh-performance devices based on conducting polymers (CPs) require the fabrication of a thick CP-coated electrode with high stability. Herein, we propose a method for enhancing the interfacial adhesion strength between a gold electrode and an electropolymerized polypyrrole (pPy) layer by introducing a polyethyleneimine (PEI) layer. Although this insulating layer hinders the initial growth of the pPy layer on the Au surface, it improves the adhesion by up to 250%. Therefore, a thick layer of pPy can be fabricated without delamination during drying. X-ray photoelectron spectroscopy shows that the PEI layer interacts with the Au surface via polar/ionic groups and van der Waals interactions. Scanning electron microscopy reveals that the cohesion of the pPy layer is stronger than the interfacial adhesion between the PEI layer and the pPy layer. Importantly, the electroactivities of pPy and its dopant are unaffected by the PEI layer, and the electrochemical storage capacity of the pPy layers on the PEI-coated Au electrodes increases with thickness, reaching ~530 mC/cm2. Negative potential sweep is detrimental to pPy layer adhesion: pPy layers on a bare Au electrode peel off instantly as the potential is swept from 0.2 to −0.7 V, and most of the charge stored in the layer becomes inaccessible. In contrast, pPy layers deposited on PEI coated Au electrode are mechanically stable and majority of the charge can be accessed, demonstrating that this method is also effective for enhancing electrochemical stability. Our simple approach can find utility in various applications involving CP-coated electrodes, where thickness-dependent performance must be improved without loss of stability.
Examining Interactions Between and Among Predictors of Net Ecosystem Exchange: A Machine Learning Approach in a Semi-arid Landscape
Authors: Zhou Qingtao , Fellows Aaron , Flerchinger Gerald N. , Flores Alejandro N. 
Published: February 2019

AbstractNet ecosystem exchange (NEE) is an essential climate indicator of the direction and magnitude of carbon dioxide (CO2) transfer between land surfaces and the atmosphere. Improved estimates of NEE can serve to better constrain spatiotemporal characteristics of terrestrial carbon fluxes, improve verification of land models, and advance monitoring of Earth’s terrestrial ecosystems. Spatiotemporal NEE information developed by combining ground-based flux tower observations and spatiotemporal remote sensing datasets are of potential value in benchmarking land models. We apply a machine learning approach (Random Forest (RF)) to develop spatiotemporally varying NEE estimates using observations from a flux tower and several variables that can potentially be retrieved from satellite data and are related to ecosystem dynamics. Specific variables in model development include a mixture of remotely sensed (fraction of photosynthetically active radiation (fPAR), Leaf Area Index (LAI)) and ground-based data (soil moisture, downward solar radiation, precipitation and mean air temperature) in a complex landscape of the Reynolds Creek Experimental Watershed (RCEW) in southwest Idaho, USA. Predicted results show good agreement with the observed data for the NEE (r2 = 0.87). We then validate the temporal pattern of the NEE generated by the RF model for two independent years at the two sites not used in the development of the model. The model development process revealed that the most important predictors include LAI, downward solar radiation, and soil moisture. This work provides a demonstration of the potential power of machine learning methods for combining a variety of observational datasets to create spatiotemporally extensive datasets for land model verification and benchmarking.
Tumour Necrosis Factor-α Inhibition Improves Stroke Outcome in a Mouse Model of Rheumatoid Arthritis
Authors: Bonetti N. R. , Diaz-Cañestro C. , Liberale L. , Crucet M. , Akhmedov A. , Merlini M. , Reiner M. F. , Gobbato S. , Stivala S. , Kollias G. , Ruschitzka F. , Lüscher T. F. , Beer J. H. , Camici G. G. 
Published: February 2019

AbstractRheumatoid Arthritis (RA) is a chronic inflammatory disorder where incidence and severity of myocardial infarction are increased. Data on the incidence and outcome of stroke are conflicting. Thus, we investigated outcome after Ischemia/Reperfusion (I/R) brain injury in a mouse model of RA and assessed for the role of the tumour necrosis factor-α (TNF-α) inhibitor Infliximab herein. We used a TNF-α reliant mouse model of RA. RA and wildtype (WT) animals were treated with vehicle (RA/WT) or Infliximab (RA Infliximab) for 4 weeks, before undergoing I/R brain injury. RA-animals displayed larger strokes and poorer neurological performance. Immunohistochemistry on brain sections revealed increased numbers of resident and peripheral innate immune cells (microglia and macrophages); increased Blood-Brain-Barrier (BBB)-disruption; decreased levels of the tight junction proteins (TJPs) claudin-5 and occludin; increased expression of matrix-metalloproteinases (MMP)-3 and -9 and enhanced lipid peroxidation. Treatment with Infliximab corrected these alterations. We show that RA associates to worse stroke-outcome via exacerbated BBB degradation by decrease of the TJPs claudin-5 and occludin. We identified MMPs-3 and -9 and increased oxidative stress as potential mediators thereof. Increased numbers of resident and peripheral innate immune cells (microglia and macrophages) may in turn contribute to all these effects. Infliximab-treatment restored the phenotype of RA-mice to baseline. Our data provide evidence clearly linking RA to adverse stroke-outcome in mice and indicate an approved TNF-α inhibitor as a potential strategy to reduce stroke-burden in this setting.
Climate change and climate change velocity analysis across Germany
Authors: Kosanic A. , Kavcic I. , van Kleunen M. , Harrison S. 
Published: February 2019

AbstractAlthough there are great concerns to what extent current and future climate change impacts biodiversity across different spatial and temporal scales, we still lack a clear information on different climate change metrics across fine spatial scales. Here we present an analysis of climate change and climate change velocity at a local scale (1 × 1 km) across Germany. We focus on seasonal climate variability and velocity and investigate changes in three time periods (1901–2015, 1901–1950 and 1951–2015) using a novel statistical approach. Our results on climate variability showed the highest trends for the 1951–2015 time period. The strongest (positive/negative) and spatially the most dispersed trends were found for Summer maximum temperature and Summer minimum temperatures. For precipitation the strongest positive trends were most pronounced in the summer (1951–2015) and winter (1901–2015). Results for climate change velocity showed that almost 90% of temperature velocities were in the range of 0.5 to 3 km/year, whereas all climate velocities for precipitation were within the range of −3.5 to 4.5 km/year. The key results amplify the need for more local and regional scale studies to better understand species individualistic responses to recent climate change and allow for more accurate future projections and conservation strategies.