bluuebunny/crossref_metadata_2025_split huggingface dataset:
Public health importance of the detection of high-grade dysplasia and adenocarcinoma lesions following 2 different protocols: High resolution Virtual chromoendoscopy versus Seattle protocol: CONVERSE study
Authors: Queneherve Lucille , Hardouin Jean-Benoit , Riche Valery-Pierre , Compaore Youssouf , ARCHAMBEAUD Isabelle , Bardot Stephanie , Alebic Iva Jirka , Marguerite Monique , Letaillandier Cyrielle , Ratajczak Christelle , Pierry Océane , Jobert Alexandra , Rahmi Gabriel , Berger Arthur , Karsenti David , Yzet Clara , Pioche Mathieu , Barrioz Thierry , Tchoumak Irina , Chevaux Jean-Baptiste , Jacques Jeremie , Wallenhorst Timothée , Vanbiervliet Geoffroy , Koch Stéphane , Barret Maximillien , Poinas Alexandra , Coron Emmanuel 
Published: November 2024

Abstract

Background: Barrett's esophagus (BE) is a pre-cancerous condition with an increased risk of esophageal adenocarcinoma (EAC). Current surveillance involves white light endoscopy with random biopsies (Seattle Protocol, SP) but has limitations. This study explores high-resolution virtual chromoendoscopy (HRMC) as a potential alternative.
Methods: This controlled trial will enroll 110 patients with Barrett's esophagus (BE) across 15 gastroenterology departments in France. Each patient will undergo both HRMC and SP examinations during the same endoscopic procedure. Although the trial is non-randomized, it is important to highlight that the two endoscopists performing either the HRMC or the SP will be blinded to each other’s results. Therefore, each patient will serve as their own control. Biopsy decisions will be based on both methods, with any visible lesions resected. The primary objective is to compare the detection rate of HGD and EAC lesions using HRMC with targeted biopsies versus SP with random biopsies. Secondary objectives include comparing detection rates of LGD lesions, procedure time, missed lesions, and cost-effectiveness.
Discussion: If HRMC proves superior to SP for detecting HGD and EAC, it could lead to more accurate, efficient, and cost-effective BE surveillance strategies, improving patient outcomes and resource utilization.
Trial Registration: ClinicalTrials.gov NCT05025826 – First Submitted 2021-12-07
Educational Escape Rooms for Raising Information Privacy Competences: An Empirical Validation
Authors: Papaioannou Thanos , Kokotos Spyridon Eftychios , Tsohou Aggeliki 
Published: November 2024

Abstract

Contemporary research has highlighted the need to specify the competences that individuals need to possess in order to protect their privacy against the potential risks of the internet, beyond mere awareness. The reassessment of conventional strategies for enhancing knowledge and skills for privacy protection has directed research attention towards the design and development of innovative educational interventions. To address this gap, in this paper we explore the effectiveness of educational escape rooms in enhancing users' privacy competencies. The study experimentally tested a 2D digital escape room which we designed to improve five specific privacy skills among 81 adult participants. The empirical findings show significant improvement in all the competencies tested: the ability to install and customize safeguards, the ability to perceive privacy risk, self-control, the ability to perceive privacy and privacy concerns. This demonstrates the potential of escape rooms as innovative tools for privacy learning, suggesting implications for designers of educational games and privacy software. This investigation contributes novel insights into privacy education by being one of the first studies to implement and test a privacy-focused escape room.
Long-term maintenance of regenerative agriculture enhances soil food webs and drives carbon accumulation
Authors: Martin Tvisha , Sprunger Christine 
Published: November 2024

Abstract

There is an expectation that regenerative agriculture will enhance soil food web structure through improved soil health, yet this has rarely been tested over long-term periods. Here, we seek to understand shifts of nematode communities and soil carbon (C) over a 30-year period across a range of agroecosystems within the W.K. Kellogg Biological Station Long-Term Ecological Research Site. After 30 years, nematode communities shifted from bacterivore and plant parasitic dominance to fungivore dominance, in unmanaged successional systems. Soil C accumulation was four times greater after 30 years, but only in successional systems. Together, this decadal study demonstrates that the long-term maintenance of regenerative agricultural practices can alter soil food web structure and drive soil C accumulation in agroecosystems.
Cellular response in the resilience of Microcystis aeruginosa under polyaluminum chloride exposure
Authors: Eyun Seong-il , Kim Eun-jeong , Park Yeon-jeong , Lee Jae Hak , Lee Heesuk , Yang Jihye , Kim Han Soon 
Published: November 2024

Abstract

Polyaluminum chloride (PAC) is a flocculant commonly used to remove microalgal cells from blooming reservoir. However, some cells exposed to PAC can survive and remain suspended at the surface of eutrophic lakes, potentially reblooming in high-temperature conditions. This study investigated the cellular responses underlying the survival resilience of Microcystis in response to PAC treatment. During cell growth, we observed that exposure to low levels of PAC led to a growth pattern resembling normal conditions, whereas cells exposed to high levels of PAC experienced immediate growth inhibition, followed by cell death. Therefore, we employed RNA sequencing to investigate dynamic gene expression. At the transcriptomic level, 264 distinct genes exhibited differential expression under low PAC exposure, significantly affecting the bacterial secretion system and photosynthesis. Changes in the expression of the photosystem II antenna complex phycobilisome were subsequently reflected in changes in phycocyanin pigment production. Furthermore, we identified 223 unique genes under high PAC exposure. Notably, in type II toxin–antitoxin systems, which serve as a prokaryotic defense mechanism, several toxin genes were expressed at higher levels than antitoxin genes, promoting cell death or apoptosis. These findings bridge a gap in the understanding of cyanobacterial ecotoxicology and environmental responses, potentially enhancing biotechnological and clinical applications.
Accuracy of the Resting Metabolic Rate Equations
Authors: Bakır Binnur Okan , Cebioğlu İrem Kaya 
Published: November 2024

Abstract

Background
The resting metabolic rate (RMR) is recommended to be measured via indirect calorimetry. Since an indirect calorimeter may not be available in all settings, predictive equations are used worldwide. Given that the accuracy of the predictive equations may depend on ethnicity, we aimed to evaluate the most accurate prediction equation in Turkish adults.
Methods
We included 57 voluntary participants (68.4% women) who were normal in body weight and overweight or obese (63.2%). Harris-Benedict; Owen; WHO/FAO/UNU weight; WHO/FAO/UNU weight and height equations; and Mifflin-St. Jeor equations were used to compare the predicted RMRs and the indirect calorimeter measurements. The root mean square error, mean percentage difference, and concordance correlation coefficient were calculated to indicate the predictivity of the equations and to show the deviation of the differences between the predicted and measured RMRs.
Results
Although it was not within the agreement of ± 10% of the measured RMR, which is acceptable for accuracy, the Harris-Benedict equation provided the highest accuracy (61.9%), whereas the Owen equation had the lowest accuracy (19%) for normal body weight. The WHO/FAO/UNU weight equation had the highest accuracy (58.3%), whereas the Owen and WHO/FAO/UNU weight and height equations had 25% accuracy for overweight and obese individuals.
Conclusions
Although none of the five widely used equations showed good accuracy in terms of our RMR measurements via indirect calorimetry, the Harris-Benedict equation was recommended for normal body weight, and the WHO/FAO/UNU weight equation was recommended for overweight and obese individuals until a new, population-based formula was developed.
Evaluation of social preference, anxiety, cortisol, and chemically-induced hyperlocomotion in mecp2 null-mutant zebrafish
Authors: Shams Soaleha , Cronell Pierre , Landin Jenny , Pietri Thomas , Gimdal Adrian Ekehorn , Kettunen Petronella , Westberg Lars 
Published: November 2024

Abstract

Background: Methyl CpG binding protein 2 (MECP2) is vital for neuronal function as it is an essential global modulator of transcription, and mutations in MECP2 are the most common cause of Rett syndrome, an X-linked neurodevelopmental disorder. Patients diagnosed with Rett syndrome have increased risk for epilepsy as well as problems with anxiety and social communication. Using the zebrafish mecp2Q63X line, this study aimed to increase our understanding of the role of mecp2 in regulation of seizures and general locomotion, ontogeny of social behaviour, and adult socialization and anxiety behaviour. To determine responses of mecp2-/- zebrafish to a stimulating convulsant, general locomotor activity was measured at 5 days post-fertilization (dpf) in sibling mecp2+/+, mecp2+/-, and mecp2-/- fish after treatment with a GABAA receptor antagonist pentylenetetrazol (PTZ) at varying concentrations. Responses to social stimulus were investigated in larval (21 dpf) and adult mecp2-/- and mecp2+/+ fish. Anxiety responses to a novel tank and whole-body cortisol levels were also measured in adult mecp2-/- and control mecp2+/+ zebrafish.
Results: The behavioural tests showed that mecp2-/- zebrafish displayed hypolocomotion at the larval stage, along with increased freezing time and thigmotaxis, and higher whole-body cortisol levels in adulthood. However, lack of functional Mecp2 did not change the hyper-locomotion response to PTZ at 5 dpf or affect the social preference for visual social stimulus at 21 dpf and in adulthood.
Conclusions: Functional mecp2 modulated larval locomotion and behavioural anxiety at different ages and adult cortisol levels, but lacking mecp2 did not alter adult locomotion or socialization, and developmental sociability and PTZ-induced hyperlocomotion in zebrafish. Given the variability reported in humans and rodent Mecp2 models, studies using zebrafish can explore vital elements of MECP2’s role across development and improve our understanding of neural mechanisms underlying neurodevelopmental disorders.
Cognitive Health After Cerebellar Stroke: Linking Socio-Educational Status and Neuropsychological Outcome
Authors: Voruz Philippe , Constantin Ioana Medeleine , Chassot Emilie , Thomasson Marine , Assal Frédéric , Péron Julie A. 
Published: November 2024

Abstract

Background
While deficits in episodic memory have been noted following cerebellar damage, there is a lack of research systematically exploring the socio-demographic and cognitive profiles of patients with such impairments.
Objective
This study aimed to differentiate between chronic-phase cerebellar stroke patients with and without verbal episodic memory deficits, and to determine whether those with deficits exhibit distinct socio-demographic and clinical profiles, thereby identifying potential factors associated with these impairments.
Methods
Data from 15 cerebellar stroke patients in the CEREBEMO cohort were analyzed, with participants categorized into two groups based on verbal episodic memory performance: deficits (n = 8) and no deficits (n = 7). Statistical analyses, including Generalized Linear Mixed Models and Chi-Squared tests, compared socio-demographic and neuropsychological variables between the groups.
Results
Significant differences were observed in socio-educational levels, with a higher proportion of patients with memory deficits at intermediate education levels. Moreover, patients with memory deficits performed worse on the Montreal Cognitive Assessment and the Trail Making Test, indicating overall lower cognitive efficiency and slower processing speed. Post-hoc analysis showed that, despite the limited sample size, our sample effectively detected a significant difference between the two groups with high statistical power.
Conclusion
These findings highlight potential socio-educational and cognitive factors associated with memory impairments following cerebellar stroke.
Low expression of miR-7-5p promotes resistance to radiotherapy in lung cancer through direct upregulation of PKP2 expression
Authors: Zhang Fan , Yang Jiaxue , Qiu Xiao-lu , He Jie , Zou Wen , Sang Yi , Chen Chun 
Published: November 2024

Abstract

Purpose
Lung cancer remains a significant global health challenge, with advanced stages often limiting surgical options and necessitating systemic therapies like radiotherapy. Resistance to radiotherapy frequently undermines treatment efficacy. This study explores the role of miR-7-5p in modulating plakophilin-2 (PKP2) expression and its impact on radiosensitivity in non-small cell lung cancer (NSCLC) cells.
Methods
Using clonogenic assays, CCK-8 assays, immunofluorescence staining, Western blotting, and reporter gene assays, we assessed the effects of miR-7-5p overexpression and inhibition in A549 NSCLC cells.
Results
Results show that miR-7-5p overexpression enhances radiosensitivity by increasing DNA damage (evidenced by higher γ-H2AX foci) and inhibiting non-homologous end joining (NHEJ) repair. Bioinformatics and experimental validation identified PKP2 as a direct target of miR-7-5p. Overexpression of PKP2 mitigates the radiosensitizing effects of miR-7-5p, confirming the miR-7-5p/PKP2 axis's role in regulating radiosensitivity.
Conclusion
This work highlights the potential of targeting the miR-7-5p/PKP2 pathway to overcome radiotherapy resistance in NSCLC, offering a promising therapeutic approach to enhance treatment outcomes.
Establishment and Validation of a Macrophage-Related Model for Predicting Synchronous Distant Metastasis of Colorectal Cancer
Authors: Li Yunlong , Guo Tairan , Huang Yuzhou , Li Yaqing , Chen Shaojie , Li Jiajia , Yin Dong , Huang Kaihong , Lian Guoda 
Published: November 2024

Abstract

Background
To search for immune cells and related risk genes that affect distant metastasis of colorectal cancer, and to construct a prediction model based on certain immune cells and risk genes to guide systemic screening for synchronous distant metastasis.
Methods
Firstly, the genes acquired from the TCGA database were screened by differential analysis, and differences in immune infiltration were analyzed by the TIMER algorithm. Then, differential genes related to immune cells were screened by association analysis. Next, LASSO regression and logistic regression were used to build a predicting model. Finally, the accuracy of the model was assessed and externally validated with ROC curves and calibration curves, and the prospect of clinical application was evaluated with the decision curve and clinical impact curve, while the potential role of selected genes in distant metastasis of colorectal cancer was verified by immunohistochemistry.
Results
The model for predicting synchronous distant metastasis of colorectal cancer is as follows: riskScore=-0.125103*C1orf35 + 0.016437*TMPRSS4 + 0.151171*PCDHGB7-0.039227*LAP3 + 0.033138*TSC22D3 + 0.031953*NIPAL2 + 0.015495*IPO5-5.642131, which is well-calibrated with an AUC value of 0.85 for internal evaluation and 0.68 for external validation, and the immunohistochemistry confirmed the potential role of seven risk genes in distant metastasis of colorectal cancer.
Conclusions
Seven macrophage-related risk genes for distant metastasis of colorectal cancer were screened, and a model was constructed to predict the incidence of synchronous distant metastasis in colorectal cancer patients as a guide for clinical PET/CT application.
On the North Atlantic Warming Hole
Authors: Madan Gaurav , Gjermundsen Ada , LaCasce Joseph H. 
Published: November 2024

Abstract

The North Atlantic Warming Hole (WH) is examined using the Norwegian Earth System model. The WH is a distinctive cool region in sea surface temperature anomaly, lying south of Iceland. It is present now, and intensifies in the climate simulation examined here. Previous studies have suggested the feature reflects declining oceanic heat transport, or increased surface cooling due to changes in the atmospheric circulation. In the present simulations, the primary driver instead is an influx of freshwater from high latitudes. The freshwater flows into the subpolar gyre and subsequently pools in the Icelandic basin. The structure cools in the interior, and as such the WH has only a weak signature in density. Rather, it has a strong "spice" anomaly, indicating density compensation. The turbulent fluxes indicate that eddy restratification acts to counteract buoyancy production from the freshwater, reducing the lateral density gradients. Thus the WH is fundamentally an advective feature in the ocean and could not be generated by atmospheric processes alone. Furthermore, the WH is not the direct result of reduced heat transport into the subpolar gyre. Rather, the freshwater simultaneously weakens the Atlantic Meridional Overturning Circulation (AMOC), so that AMOC declines in concert with the cooling of the NAWH. The findings underscore the significant role of freshwater fluxes from high latitudes, from melting Arctic sea ice and increased runoff, in altering the regional and global ocean circulation.
Emotion regulation beyond executive and attention difficulties: impact on daily life impairments in typically developing adolescents
Authors: Poznyak Elena , Debbané Martin 
Published: November 2024

Abstract

Background It is becoming widely recognized that emotion dysregulation is an essential feature present along the continuum from subclinical to clinical Attention Deficit/Hyperactivity Disorder (ADHD). Yet, it remains unclear whether and how specific processes related to emotion regulation contribute to daily life impairments, across different domains of functioning. The aim of this cross-sectional study in community adolescents was to investigate whether three processes commonly implicated in adaptive emotion regulation – emotion recognition, emotion reactivity and use of cognitive emotion regulation strategies – uniquely contribute to adolescent-rated functional impairment, above and beyond the effects of age and gender, ADHD symptoms, and individual differences in verbal ability and executive control. Methods 161 adolescents from the general population (mean age = 15.57; SD = 1.61) completed the Weiss Functional Impairment Scale, the Emotion Reactivity Scale, the Cognitive Emotion Regulation Questionnaire and the Geneva Emotion Recognition Test. Hierarchical regression analysis examined the unique contributions of candidate predictors to impairment scores. Results Total impairment scores were best predicted by older age, inattention symptoms, higher emotional reactivity, and higher use of maladaptive cognitive emotion regulation strategies. Emotion regulation processes were associated with interpersonal difficulties and self-concept impairments, whereas inattention symptoms were associated with school and life skills impairments. Conclusions This study stresses that emotion reactivity and maladaptive cognitive emotion regulation represent major sources of perceived social and emotional difficulties in community adolescents. Our results also support the continuum hypothesis of attention difficulties, where emotion regulation abilities may at least partially explain the relationship between ADHD symptoms and social impairments. Together, these findings highlight the vital importance of targeting emotion regulation in psychotherapeutic interventions aiming to improve socio-emotional outcomes in adolescents.
Awareness and Usage of Electronic Resources Among University Students: A Case Study of Ogongo Campus, University of Namibia
Authors: Uukongo Wilka Nandjambi , Beukes Maggy Amiss Dr , Uutoni Wilhem Dr 
Published: November 2024

Abstract

This study investigates the awareness and usage of electronic resources (e-resources) among students at the University of Namibia's Ogongo Campus. Using a qualitative phenomenological approach, the research aims to determine the level of awareness, identify available e-resources, assess usage levels, and understand the challenges faced by students in utilizing these resources. Data were collected through focus group discussions, semi-structured interviews, and observations. The findings reveal that while some students are aware of the available e-resources, many struggles with accessibility and usage due to various challenges. The study recommends enhanced library awareness campaigns and training programs to improve students' digital literacy and e-resource utilization.
Isolation and characterization of goose astrovirus genotype 1 causing enteritis in goslings from Sichuan province, China
Authors: Chen Guo , Zhang Huanrong 
Published: November 2024

Abstract

Since 2017, Goose astrovirus (GoAstV) has been widely prevalent in various provinces of China, leading to substantial economic losses in the goose industry. This study documents an outbreak of infectious disease in 1–3 week-old goslings on a goose farm in Sichuan, characterized by enteritis and a mortality rate of approximately 30%. Viral metagenomic sequencing of fecal samples identified the infection as Goose Astrovirus genotype 1 (GoAstV-1). PCR analysis further confirmed GoAstV-1 in liver tissues from deceased goslings, and a GoAstV-1 strain was successfully isolated from goose embryos. The full genome of the isolated strain, named Goose Astrovirus AAstV/Goose/CHN/2023/C1 (GenBank No. PP108251), was obtained through segmental amplification, sequencing, and assembly. Phylogenetic analysis revealed that the isolate belongs to GoAstV-1 subtype B. Experimental inoculation of one-day-old goslings with the virus (10− 4.834 ELD50/0.5 mL) resulted in a mortality rate of 5 out of 15 and a reduction in growth rates. Pathological examination indicated viral damage to the intestines, liver, spleen, and kidneys. These findings contribute to a deeper understanding of the genetic evolution and pathogenicity of GoAstV-1 and provide critical insights for disease prevention and control strategies in Sichuan province.
Metallo-serine protease mediated synthesis of silver nanoparticles with enhanced therapeutic properties
Authors: Khan Zahoor , Shafique Maryam , Khan Sumera Afzal , Hayat Fawad , Jabeen Nusrat , Naz Sehar Afshan 
Published: November 2024

Abstract

Silver nanoparticles (AgNPs) possess distinctive physicochemical properties which make them well-suited for various commercial applications. Herein, we developed a single-step and efficient technique for AgNPs biosynthesis using a metallo-serine protease as a bio-reducing agent employing hydrothermal method. The optimal conditions for AgNPs biosynthesis were 600 U mL⁻¹ enzyme concentration and 1.25% (5 mM) silver nitrate, with steam sterilization for 2 min at 121 °C and 15 psi. During characterization, Scanning Electron Microscopy and Energy Dispersive X-ray Spectroscopy revealed spherical particles with silver peaks at 3.00-3.5 keV and counts of ~50-350. X-ray Diffraction indicated a crystalline structure with an average particle size of 30.07 nm, while Fourier-transform Infrared Spectroscopy confirmed the presence of functional groups of carboxylic acids, amine salts, and alkyl aryl ethers, involved in bio-capping and stabilization of the nanoparticles. The particles demonstrated significant antimicrobial activity against clinical isolates including Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhimurium, Klebsiella pneumoniae, Morganella morganii, and Enterococcus faecalis at 100 μg mL⁻¹. Moreover, AgNPs also exhibited notable radical scavenging activity of 68.15% at 500 μg mL⁻¹, suggesting their potential antitumor properties. These findings present protease-assisted hydrothermal synthesis as an alternative method for producing metal nanoparticles with extended therapeutic properties.