bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross‑cultural adaptation and psychometric properties of the Indonesia version of the self‐efficacy oral health questionnaire for adolescents
Authors: Rachmawati Yuanita Lely , Maharani Diah Ayu , Oho Takahiko 
Published: February 2019

SummaryBackgroundSelf‐efficacy can be interpreted as a self‐belief that one has successfully committed to a specific situation or maintained a specific behaviour.AimWe aimed to test the reliability and validity of a cross‐cultural adaptation of the self‐efficacy oral health (SEOH) questionnaire in the Indonesian language among adolescents and to understand the diverse perspectives of oral health self‐efficacy related to gender.DesignA cross‐cultural adaptation of the SEOH self‐administered questionnaire was completed followed by a dental caries examination. Sample size estimation suggested 341 adolescents aged 12‐13 years. Eleven junior high schools were clustered and randomly selected from six districts across Jakarta, Indonesia.ResultsThe SEOH was reliable with interclass correlation and Cronbach's alpha values of 0.86 and 0.86, respectively. Significant results for the construct and discriminant validity analyses were observed. There was no significant difference in terms of the total score between males and females, but there was a significant difference in terms of the psychology control domain.ConclusionsOur study results provide evidence supporting the reliability and validity of the Indonesian version of the SEOH questionnaire for adolescents across Indonesia.
The psychometric properties of the CFSS‐DS for schoolchildren in Saudi Arabia: A confirmatory factor analytic approach
Authors: Alharbi Amjad , Humphris Gerry , Freeman Ruth 
Published: February 2019

SummaryAimTo test the psychometric properties of an Arabic version of the Child Fear Survey Schedule‐Dental Subscale (CFSS‐DS) a using confirmatory factor analysis.MethodsTwo convenience samples were obtained: Sample [1]: 600 boys (33%) and girls attending 4 public schools in Onizah and Sample [2] 800 girls attending 8 public schools in Buridah. The questionnaire asked the participant's age, gender, and completion of the CFSS‐DS. The data were subjected to exploratory factor analysis (EFA), parallel factor analysis, Cronbach's alpha, confirmatory factor analysis (CFA), and goodness of fit statistics.ResultsA total of 513 children in Sample [1] and 503 children in Sample [2] participated giving a valid response rate of 86% and 67%, respectively. From the EFA, 3 factors were identified and confirmed statistically using parallel factor analysis. The internal consistency of the 3 factors, Dental Fear Subscale (0.86), Hospital Fear Subscale (0.77), and Stranger Fear Subscale (0.71), was good. The CFA showed that the current EFA model was an equivalent fit to the El Housseiny et al (BMC Oral Health 2016;16:49). model; however, the solution using El Housseiny et al's structure was distorted.ConclusionsA 3‐factor structure with acceptable reliability exists for this Arabic version of the CFSS‐DS, confirmed by a CFA using an additional data set.
Characteristics of ventricular intracardiac electrograms of ventricular tachycardias originating from the epicardia in patients with an implantable cardioverter defibrillator
Authors: Kawamura Iwanari , Fukamizu Seiji , Arai Marina , Inagaki Dai , Miyabe Tomonori , Miyazawa Satoshi , Kitamura Takeshi , Hojo Rintaro , Nishizaki Mitsuhiro , Sakurada Harumizu , Hiraoka Masayasu 
Published: February 2019

AbstractIntroductionWhile characteristic waveforms of 12‐lead electrocardiograms have been reported to predict the epicardial origin of ventricular tachycardia (VT), it has not been fully examined whether ventricular intracardiac electrograms (VEGMs) recorded from the implantable cardioverter defibrillator (ICD) via telemetry can determine the origin of VT or not. The aim of this study was to investigate the VEGM characteristics of VT originating from the epicardia.Method and ResultsIntracardiac VEGMs of the induced VTs, with detected sites of origin during the VT study, were recorded in 15 (23 VTs) of the 46 patients. The characteristics of the 23 VTs were evaluated using far‐field and near‐field VEGMs recorded via telemetry. Five of 23 VTs were found to be focused on the epicardial site (epi group) and 18 VTs were focused on the endocardium (endo group). VTs of the epi group had longer VEGM duration in far‐field EGM than those of the endo group (epi group: 240 ± 49 ms vs endo group: 153 ± 45 ms; P = 0.002) and the duration from the onset to the peak of VEGM was also longer than that of the endo group (epi group: 153 ± 53 ms vs endo group: 63 ± 28 ms; P < 0.001). There was no difference in the V wave duration in tip‐ring EGM between both groups (epi group: 122 ± 52 ms vs endo group: 98 ± 6 ms; P = 0.377).ConclusionEvaluation of intracardiac VEGM before VT ablation may be helpful to predict the epicardial origin of VT in patients with an ICD.
An integrated analysis to predict micro‐RNAs targeting both stemness and metastasis in breast cancer stem cells
Authors: Rahimi Mahsa , Sharifi‐Zarchi Ali , Firouzi Javad , Azimi Mahnaz , Zarghami Nosratollah , Alizadeh Effat , Ebrahimi Marzieh 
Published: February 2019

AbstractSeveral evidences support the idea that a small population of tumour cells representing self‐renewal potential are involved in initiation, maintenance, metastasis, and outcomes of cancer therapy. Elucidation of microRNAs/genes regulatory networks activated in cancer stem cells (CSCs) is necessary for the identification of new targets for cancer therapy. The aim of the present study was to predict the miRNAs pattern, which can target both metastasis and self‐renewal pathways using integration of literature and data mining. For this purpose, mammospheres derived from MCF‐7, MDA‐MB231, and MDA‐MB468 were used as breast CSCs model. They had higher migration, invasion, and colony formation potential, with increasing in stemness‐ and EMT‐related genes expression. Our results determined that miR‐204, ‐200c, ‐34a, and ‐10b contemporarily could target both self‐renewal and EMT pathways. This core regulatory of miRNAs could increase the survival rate of breast invasive carcinoma via up‐regulation of OCT4, SOX2, KLF4, c‐MYC, NOTCH1, SNAI1, ZEB1, and CDH2 and down‐regulation of CDH1. The majority of those target genes were involved in the regulation of pluripotency, MAPK, WNT, Hedgehog, p53, and transforming growth factor β pathways. Hence, this study provides novel insights for targeting core regulatory of miRNAs in breast CSCs to target both self‐renewal and metastasis potential and eradication of breast cancer.
Acetyltransferase p300 inhibitor reverses hypertension‐induced cardiac fibrosis
Authors: Rai Rahul , Sun Tianjiao , Ramirez Veronica , Lux Elizabeth , Eren Mesut , Vaughan Douglas E. , Ghosh Asish K. 
Published: February 2019

AbstractEpigenetic dysregulation plays a crucial role in cardiovascular diseases. Previously, we reported that acetyltransferase p300 (ATp300) inhibitor L002 prevents hypertension‐induced cardiac hypertrophy and fibrosis in a murine model. In this short communication, we show that treatment of hypertensive mice with ATp300‐specific small molecule inhibitor L002 or C646 reverses hypertension‐induced left ventricular hypertrophy, cardiac fibrosis and diastolic dysfunction, without reducing elevated blood pressures. Biochemically, treatment with L002 and C646 also reverse hypertension‐induced histone acetylation and myofibroblast differentiation in murine ventricles. Our results confirm and extend the role of ATp300, a major epigenetic regulator, in the pathobiology of cardiac hypertrophy and fibrosis. Most importantly, we identify the efficacies of ATp300 inhibitors C646 and L002 in reversing hypertension‐induced cardiac hypertrophy and fibrosis, and discover new anti‐hypertrophic and anti‐fibrotic candidates.
Type 2 diabetes is associated with suppression of autophagy and lipid accumulation in β‐cells
Authors: Ji Jeff , Petropavlovskaia Maria , Khatchadourian Armen , Patapas Jason , Makhlin Julia , Rosenberg Lawrence , Maysinger Dusica 
Published: February 2019

AbstractBoth type 2 diabetes (T2D) and obesity are characterized by excessive hyperlipidaemia and subsequent lipid droplet (LD) accumulation in adipose tissue. To investigate whether LDs also accumulate in β‐cells of T2D patients, we assessed the expression of PLIN2, a LD‐associated protein, in non‐diabetic (ND) and T2D pancreata. We observed an up‐regulation of PLIN2 mRNA and protein in β‐cells of T2D patients, along with significant changes in the expression of lipid metabolism, apoptosis and oxidative stress genes. The increased LD buildup in T2D β‐cells was accompanied by inhibition of nuclear translocation of TFEB, a master regulator of autophagy and by down‐regulation of lysosomal biomarker LAMP2. To investigate whether LD accumulation and autophagy were influenced by diabetic conditions, we used rat INS‐1 cells to model the effects of hyperglycaemia and hyperlipidaemia on autophagy and metabolic gene expression. Consistent with human tissue, both LD formation and PLIN2 expression were enhanced in INS‐1 cells under hyperglycaemia, whereas TFEB activation and autophagy gene expression were significantly reduced. Collectively, these results suggest that lipid clearance and overall homeostasis is markedly disrupted in β‐cells under hyperglycaemic conditions and interventions ameliorating lipid clearance could be beneficial in reducing functional impairments in islets caused by glucolipotoxicity.
Resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition
Authors: Zou Yanfen , Li Shuhong , Wu Dan , Xu Yetao , Wang Sailan , Jiang Ying , Liu Fang , Jiang Ziyan , Qu Hongmei , Yu Xiang , Wang Xiaoli , Wang Yuanli , Sun Lizhou 
Published: February 2019

AbstractImpairment spiral arteries remodelling was considered to be the underlying cause of pathogenesis of pre‐eclampsia (PE). Resveratrol (RE) was reported that it could modulate cellar phenotype to ameliorate diverse human diseases. However, the biological function of RE in PE remains poorly understood. In this report, we investigated the effect of RE on trophoblast phenotype both in vivo and in vitro. We conducted MTT and transwell assays to explore cell proliferation and invasion events in HTR‐8/SVneo. In mice model, the clinical characteristics of PE were established through the injection of NG‐nitro‐l‐arginine methyl ester (L‐NAME). Furthermore, related experiments were performed to detect cellar phenotype‐associated signalling pathway, including epithelial‐mesenchymal transition (EMT) and Wnt/β‐catenin. Cell assays indicated that RE could increase trophoblasts migration and invasion. In addition, hypertension and proteinuria were markedly ameliorated by RE compared with the controls in PE mice model. Moreover, treatment by RE in trophoblasts or in PE model, we found that RE activated EMT progress through the regulation of E‐cadherin, β‐catenin, N‐cadherin, vimentin expression, and further altered the WNT‐related gene expression, including WNT1, WNT3 and WNT5B. Our findings demonstrated that RE might stimulate the invasive capability of human trophoblasts by promoting EMT and mediating the Wnt/β‐catenin pathway in PE.
Comparison between two bone substitutes for alveolar ridge preservation after tooth extraction: Cone‐beam computed tomography results of a non‐inferiority randomized controlled trial
Authors: Llanos Alexandre Hugo , Sapata Vítor Marques , Jung Ronald E. , Hämmerle Christoph H. , Thoma Daniel S. , César Neto João Batista , Pannuti Claudio Mendes , Romito Giuseppe Alexandre 
Published: February 2019

AbstractAimTo test the non‐inferiority of demineralized bovine bone mineral (DBBM) compared toDBBMwith 10% collagen (DBBM‐C) for maintenance of bone volume after tooth extraction in the anterior maxilla.Materials and MethodsSixty‐six patients were randomly treated withDBBMorDBBM‐C, both of which were covered with a collagen matrix for ridge preservation in the anterior maxilla. Cone‐beam computed tomographic analysis was performed immediately and 4 months after treatment. The primary outcome, for which non‐inferiority ofDBBMwas tested, was change in the horizontal ridge width 1 mm below the buccal alveolar crest (HW‐1) 4 months after extraction.ResultsFour months after extraction,HW‐1 measured −1.60 mm ± 0.82 mm forDBBM‐C, while theDBBMgroup showed a mean loss of −1.37 mm ± 0.84 mm (p = 0.28, 0.23 [95%CI: −0.19; 0.64]). The horizontal ridge width at 3 mm (HW‐3) showed −0.98 mm (±0.67 mm) forDBBM‐C and −0.84 mm (±0.62 mm) forDBBM(p = 0.40, 0.12 [95%CI: −0.19; 0.45]), and the horizontal ridge width at 5 mm (HW‐5) showed −0.67 mm (±0.47 mm) forDBBM‐C and −0.56 mm (±0.48 mm) forDBBM(p = 0.36, 0.11 [95%CI: −0.13; 0.34]).ConclusionsThe present clinical trial demonstrated non‐inferiority ofDBBMcompared toDBBM‐C for maintenance of alveolar bone volume 4 months after tooth extraction in the anterior maxilla.
Leucine–glycine and carnosine dipeptides prevent diabetes induced by multiple low‐doses of streptozotocin in an experimental model of adult mice
Authors: Vahdatpour Tohid , Nokhodchi Ali , Zakeri‐Milani Parvin , Mesgari‐Abbasi Mehran , Ahmadi‐Asl Naser , Valizadeh Hadi 
Published: February 2019

AbstractAims/IntroductionPeptides are considered to be quasi‐hormones and effective molecules for regulation of the cells function and prevention of metabolic disorders. Di‐ and tripeptides gastrointestinal absorption ability have been proposed to prevent diabetes progression.Materials and MethodsSmall peptides with different sequences of specific amino acids were synthesized based on a solid phase peptide synthesis protocol, and carnosine (A) and glutathione were examined for the prevention of diabetes induced by multiple low‐doses of streptozotocin in mice.ResultsThe peptides A, Leu‐Gly (D) and Pro‐Pro showed preventive effects on blood glucose elevation and impairment of the signaling and performance of β‐cells. The β‐cell function assessed by immunofluorescence and blood glucose level in mice exposed to diabetes treated by the peptides A and D was similar to the normal mice. The peptide D prevented bodyweight loss caused by diabetes induction. The use of D and A peptides dramatically prevented the incidence of disruption in β‐cells signaling by maintaining the natural balance of intracellular Akt‐2 and cyclic adenosine monophosphate.ConclusionsThe results proved that peptide D (Leu‐Gly), named Hannaneh, inhibits the bodyweight loss caused by diabetes induction. The Hannaneh and carnosine dipeptides, with preservation of normal β‐cell signaling and anti dipeptidyl peptidase‐4 activity, prevented blood glucose increases in mice at risk of diabetes. These dipeptides might be regarded as the pharmaceutical agents for the prevention of diabetes.
Efficacy of photodynamic therapy combined with topical interventions for the treatment of actinic keratosis: a meta‐analysis
Authors: Heppt M.V. , Steeb T. , Leiter U. , Berking C. 
Published: February 2019

AbstractBackgroundPhotodynamic therapy (PDT) is a highly effective treatment option for patients with actinic keratoses (AK). However, efficacy can be reduced by insufficient illumination or hyperkeratotic nature of lesions.ObjectivesTo investigate if PDT combined with a topical intervention is superior to monotherapy in terms of efficacy and tolerability.MethodsA systematic literature research was conducted in Medline, Embase and CENTRAL. Pertinent trial registers were hand‐searched for eligible randomized controlled trials (RCTs) until 20 August 2018. Results were pooled using a random effects model to calculate relative risks (RR) or mean differences. The risk of bias was assessed with the Cochrane Risk of Bias Tool. The quality of evidence was estimated for each outcome of interest according to GRADE.ResultsOut of 1800 references initially identified, 10 RCTs with a total sample size of n = 277 were included. Four studies investigated a combination of PDT with imiquimod cream, three with 5‐fluorouracil cream and one each with ingenol mebutate gel, tazarotene gel and calcipotriol ointment, respectively. Patients treated with a combination showed higher participant complete (RR 1.63; 95% CI 1.15‐2.33; P = 0.007) and partial clearance rates (RR 1.19; 95% CI 0.84–1.67; P = 0.33). Similarly, the lesion‐specific clearance was higher for PDT plus topical intervention compared to monotherapy (RR 1.48; 95% CI 1.04–2.11; P = 0.03). A subgroup analysis was performed for PDT combined with imiquimod, revealing an increased participant complete clearance rate compared to monotherapy (RR 1.57, 95% CI 1.09–2.25, P = 0.02). PDT‐induced pain and local skin reactions after treatment were poorly reported. The studies were estimated at high risk for performance and detection bias.ConclusionThe combination of PDT with another topical drug intervention does improve AK clearance rates compared to either monotherapy alone. This study highlights that the sequential application of two field‐directed treatments represents an efficient approach in patients with multiple AK and field cancerization.
Challenges in creating dissectible anatomical 3D prints for surgical teaching
Authors: Ratinam Ratheesraj , Quayle Michelle , Crock John , Lazarus Michelle , Fogg Quentin , McMenamin Paul 
Published: February 2019

AbstractThree‐dimensional (3D) printing, or additive manufacturing, is now a widely used tool in pre‐operative planning, surgical teaching and simulator training. However, 3D printing technology that produces models with accurate haptic feedback, biomechanics and visuals for the training surgeon is not currently available. Challenges and opportunities in creating such surgical models will be discussed in this review paper. Surgery requires proper tissue handling as well as knowledge of relevant anatomy. To prepare doctors properly, training models need to take into account the biomechanical properties of the anatomical structures that will be manipulated in any given operation. This review summarises and evaluates the current biomechanical literature as it relates to human tissues and correlates the impact of this knowledge on developing high fidelity 3D printed surgical training models. We conclude that, currently, a printer technology has not yet been developed which can replicate many of the critical qualities of human tissue. Advances in 3D printing technology will be required to allow the printing of multi‐material products to achieve the mechanical properties required.
Yield and quality of grapes cultivated under plastic coverings with different downy mildew control strategies
Authors: Holcman Ester , Sentelhas Paulo Cesar , Spósito Marcel Bellato , Conceição Marco Antônio Fonseca , Couto Hilton Thadeu Zarate 
Published: February 2019

AbstractViticulture has been expanding in tropical regions. However, the climate in these areas is generally favourable to the incidence of plant diseases, especially downy mildew. Plastic covers and warning systems have shown very positive results in disease control, but they are tools that have never been used simultaneously in a tropical area. The Vitis vinifera cv. BRS Morena table grape was evaluated as regards yield and quality under different downy mildew control strategies as carried out on vineyards trained on an overhead trellis system, covered by a black shading screen (BSS) or a braided polypropylene film (BPF), over a 3‐year period. Different grapevine downy mildew management approaches defined the treatments: Co) Control (no spraying); Ca) Conventional control (calendar); Ba) “Rule 3–10” (Atti Istituto Botanico, 8, 1947, 45); Ma25) Low‐infection efficiency—i0>25%; and Ma75) High‐infection efficiency—i0>75% (Plant Disease, 84, 2000, 549). The occurrence of downy mildew and the amount of damage inflicted on vine yield and grape quality are directly related to the period of the crop cycle when there is rainfall. The use of the Ma75 warning system (Plant Disease, 84, 2000, 549) under braided polypropylene film resulted, for the most part, in similar vineyard productivity compared to Ca, but did not influence the number of branches and its fertility. The other warning systems decreased productivity by 31.9% compared to Ca. It was not possible to establish a relationship between the occurrence of downy mildew and its influence on grape sweetness and acidity. The use of warning systems led to a substantial reduction in fungicide sprays, approximately 66.7 to 71.3%, compared to the calendar system commonly used by the vine growers, with the Ba (Atti Istituto Botanico, 8, 1947, 45) and Ma75 controls (Plant Disease, 84, 2000, 549) leading to the highest fungicide saving.
How floral displays affect geitonogamy in an upward foraging bumblebee‐pollinated protandrous plant
Authors: Zhu Xing‐Fu , Yang Ji‐Qin , Li Qing‐Jun 
Published: February 2019

AbstractReducing geitonogamy (pollen transfer among flowers within the same plant) has been suggested as a major selective force for plants with multiple flowers. The occurrence of geitonogamy is generally different among flowers within inflorescences; however, no researchers have examined whether plants enlarge their display size without increasing the possibility of geitonogamy by presenting more flowers at positions where they are less likely to be geitonogamously pollinated. We observed that bumblebee pollinators foraged upward within the tower‐shaped inflorescences of protandrous Megacodon stylophorus (C. B. Clarke) Harry Sm. Because M. stylophorus did not strictly bloom bottom‐up, there were substantial frequencies of geitonogamous pollination resulting from upward and horizontal pollen transfer. Although there was a strong correlation between total numbers of flowers plants produced and numbers of flowers presented on single days, proportions of flowers possibly geitonogamously pollinated were weakly correlated with total numbers of flowers. This might have been because plants with more resources enlarged their display size by producing more flowers on lower floors where flowers had a low probability of being geitonogamously pollinated. This study shows that the tower‐shaped inflorescences of M. stylophorus enlarge their size without more cost of geitonogamous mating, suggesting that geitonogamy acts as an important selective agent in the evolution of inflorescence architectures.
Controlling chronic Pseudomonas aeruginosa infections by strategically interfering with the sensory function of SagS
Authors: Dingemans Jozef , Al‐Feghali Rebecca E. , Lau Gee W. , Sauer Karin 
Published: February 2019

SummaryThe hybrid sensor SagS plays a central role in the formation of Pseudomonas aeruginosa biofilms, by enabling the switch from the planktonic to the biofilm mode of growth and by facilitating the transition of biofilm cells to a highly tolerant state. In this study, we examined the importance of the SagS key amino acid residues associated with biofilm formation (L154) and antibiotic tolerance (D105) in P. aeruginosa virulence. Recombinant P. aeruginosa ΔsagS and ΔsagS chromosomally expressing wild‐type sagS, or its two variants D105A and L154A, were tested for their potential to form biofilms and cause virulence in plants and mouse models of acute and chronic pneumonia. Although mutation of sagS did not alter P. aeruginosa virulence during acute infections, a significant difference in pathogenicity of sagS mutants was observed during chronic infections, with the L154A variant showing reduced bacterial loads in the chronic pneumonia model, while interference with the D105 residue enhanced the susceptibility of P. aeruginosa biofilms during tobramycin treatment. Our findings suggest that interference with the biofilm or tolerance regulatory circuits of SagS affects P. aeruginosa pathogenicity in chronic but not acute infections, and reveal SagS to be a promising new target to treat P. aeruginosa biofilm infections.