bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biologi, neraca hayati, dan pemangsaan Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) pada Paracoccus marginatus Williams & Granara de Willink (Hemiptera: Pseudococcidae)
Authors: Wahyuningsih Eka , Rauf Aunu , Santoso Sugeng 
Published: August 2019

Kutu putih pepaya, Paracoccus marginatus Williams & Granara de Willink (Hemiptera: Pseudococcidae), adalah hama asing invasif yang terdeteksi untuk pertama kalinya di Indonesia pada tahun 2008. Salah satu musuh alami yang umum ditemukan adalah kumbang predator Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae). Penelitian ini bertujuan untuk menentukan parameter biologi dan neraca hayati serta pemangsaan C. montrouzieri pada P. marginatus. Pengamatan biologi dan neraca hayati serta pemangsaan dilakukan dengan menempatkan predator C. montrouzieri di dalam cawan Petri yang berisi kutu putih. P. marginatus. Hasil penelitian menunjukkan bahwa rataan masa inkubasi telur C. montrouzieri adalah 4,35 hari. Perkembangan larva instar-1, instar-2, instar-3, dan instar-4 secara berurutan berlangsung 4,23; 4,21; 5,84; dan 5,93 hari. Prapupa berlangsung 1,95 hari dan pupa 7,55 hari. Masa hidup imago jantan adalah 51,34 hari, sedangkan betina 83,39 hari. Jumlah telur yang diletakkan oleh satu imago betina rata-rata 198,49 butir. Laju reproduksi bersih (Ro) 117,11 individu/betina/generasi, laju pertambahan intrinsik (rm) 0,073 individu/betina/hari, rataan masa generasi (T) 64,776 hari, masa penggandaan (Dt) 9,426 hari, dan laju pertambahan terbatas (λ) adalah 1,076 per hari. Larva instar-4 C. montrouzieri merupakan fase yang paling rakus, dengan rataan jumlah telur, nimfa instar-1, instar-2, instar-3, dan imago betina kutu putih yang dimangsa per hari berturut-turut 188,0 butir; 53,4; 44,0; 26,8; dan 15,6 individu. Indeks preferensi Manly menunjukkan bahwa larva dan imago C. montrouzieri lebih memilih telur dan nimfa instar-1 P. marginatus dibandingkan dengan fase mangsa lainnya. Hasil penelitian mengindikasikan potensi yang tinggi dari C. montrouzieri dalam pengendalian hayati P. marginatus.
Diet and wild ungulate preferences of wolves in northwestern Anatolia during winter
Authors: Mengüllüoğlu Deniz , İlaslan Eylül , Emir Hasan , Berger Anne 
Published: August 2019

The gray wolf (Canis lupus) is making a comeback in many habitats in central Europe, where it has been once extirpated. Although densities are still low to moderate, this comeback already raises management concerns. In Anatolia, the gray wolf is one of the most common predator species occupying almost all kind of habitats. Although its numbers were reduced in some parts of the country, it has never been extirpated and lived in sympatry with humans. In this study we investigated, for the first time, the winter diet of wolves in north-west Anatolia, where a multispecies wild ungulate community occurs in sympatry with high density livestock. We selected two geographically close but different habitats (steppe and forest) with different wild prey availabilities and compositions. In both areas ungulate contribution to winter diet biomass was more than 90%. Wolf pack size (four to eight wolves) were higher in the study area where livestock numbers and human disturbance were lower and wild prey were more available. In both study areas, wild boar (Sus scrofa) was the main and most preferred food item (Chesson’s α = 0.7 − 0.9) and it occurred at higher density where wolf pack size was smaller. We could not find a high preference (Chesson’s α = 0.3) and high winter predation pressure on the reintroduced Anatolian wild sheep (Ovis gmelinii anatolica) population that occurs in the study area covered by steppe vegetation. Contribution of livestock and food categories other than wild ungulates to wolf diet stayed low. Wolves can help mitigate human-wildlife conflict regulating wild boar numbers, the most common conflict-causing ungulate species in Anatolia. Instead of managing wolf numbers in human dominated landscapes, we recommend reintroduction of wild ungulates to the areas where they became locally extinct and replaced by livestock.
Genome-wide identification and expression analysis of the VQ gene family in soybean (Glycine max)
Authors: Wang Yongbin , Jiang Zhenfeng , Li Zhenxiang , Zhao Yuanling , Tan Weiwei , Liu Zhaojun , Cui Shaobin , Yu Xiaoguang , Ma Jun , Wang Guangjin , Li Wenbin 
Published: August 2019

Background
VQ proteins, the plant-specific transcription factors, are involved in plant development and multiple stresses; however, only few articles systematic reported the VQ genes in soybean.

Methods
In total, we identified 75 GmVQ genes, which were classified into 7 groups (I-VII). Conserved domain analysis indicated that VQ gene family members all contain the VQ domains. VQ genes from the same evolutionary branches of soybean shared similar motifs and structures. Promoter analysis revealed that cis-elements related to stress responses, phytohormone responses and controlling physical as well as reproductive growth. Based on the RNA-seq and qRT-PCR analysis, GmVQ genes were showed expressing in nine tissues, suggesting their putative function in many aspects of plant growth and development as well as response to stress in Glycine max.

Results
This study aims to understand the roles of VQ genes in various development processes and their expression patterns in responses to stimuli. Our results provide basic information in identification and classification of GmVQ genes. Further experimental analysis will allows us to know the functions of GmVQs participation in plant growth and stress responses.
Nutritional Quality, Functional Properties and Sensory Acceptability of an Orange - fleshed Sweet Potato - based Complementary Food
Authors: Ashun Esther Kumea , Darkwa Sarah , Nsiah-Asamoah Christiana 
Published: August 2019

The study focuses on the development of a complementary food (CF) with the orange-fleshed sweet potato (OFSP) to help address the public health problem of vitamin A deficiency among infants. Experimental research design was used for the study. Fifty six infants aged between 6 and 24 months were purposively sampled, together with their mothers, to evaluate three complementary food (CF) products and a control, code-named GAD, PEA, SAB and KAN respectively. The CF products were formulated from orange fleshed sweet potato, anchovies, onion and tomatoes; and the nutrients and functional properties were determined. A questionnaire and an interview guide were used to collect data to assess the sensory attributes and overall acceptability of the formulated CF products. The results showed that the three complementary foods were nutrient dense, with the moisture content being highest in PEA and lowest in GAD. GAD, PEA and SAB were all high in protein and fibre but low in fat and carbohydrate. KAN (control) was the most acceptable with respect to all the sensory attributes of a complementary food, although its overall acceptability was not significantly different from that of the other three CF products - GAD, PEA and SAB.
A recurrent 8 bp frameshifting indel in FOXF1 defines a novel mutation hotspot associated with alveolar capillary dysplasia with misalignment of pulmonary veins
Authors: Karolak Justyna A. , Bacolla Albino , Liu Qian , Lantz Patrick E. , Petty John , Trapane Pamela , Panzer Karin , Totapally Balagangadhar R. , Niu Zhiyv , Xiao Rui , Xie Nina G. , Wu Lucia R. , Szafranski Przemyslaw , Zhang David Y. , Stankiewicz Paweł 
Published: August 2019

AbstractAlveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) is a rare lethal lung developmental disease. Affected infants manifest with severe respiratory distress and refractory pulmonary hypertension and uniformly die in the first month of life. Heterozygous point mutations or copy‐number variant deletions involving FOXF1 and/or its upstream lung‐specific enhancer on 16q24.1 have been identified in the vast majority of ACDMPV patients. We have previously described two unrelated families with a de novo pathogenic frameshift variant c.691_698del (p.Ala231Argfs*61) in the exon 1 of FOXF1. Here, we present a third unrelated ACDMPV family with the same de novo variant and propose that a direct tandem repeat of eight consecutive nucleotides GCGGCGGC within the ~4 kb CpG island in FOXF1 exon 1 is a novel mutation hotspot causative for ACDMPV.
2019 Update of the American College of Rheumatology/Spondylitis Association of America/Spondyloarthritis Research and Treatment Network Recommendations for the Treatment of Ankylosing Spondylitis and Nonradiographic Axial Spondyloarthritis
Authors: Ward Michael M. , Deodhar Atul , Gensler Lianne S. , Dubreuil Maureen , Yu David , Khan Muhammad Asim , Haroon Nigil , Borenstein David , Wang Runsheng , Biehl Ann , Fang Meika A. , Louie Grant , Majithia Vikas , Ng Bernard , Bigham Rosemary , Pianin Michael , Shah Amit Aakash , Sullivan Nancy , Turgunbaev Marat , Oristaglio Jeff , Turner Amy , Maksymowych Walter P. , Caplan Liron 
Published: August 2019

ObjectiveTo update evidence‐based recommendations for the treatment of patients with ankylosing spondylitis (AS) and nonradiographic axial spondyloarthritis (SpA).MethodsWe conducted updated systematic literature reviews for 20 clinical questions on pharmacologic treatment addressed in the 2015 guidelines, and for 26 new questions on pharmacologic treatment, treat‐to‐target strategy, and use of imaging. New questions addressed the use of secukinumab, ixekizumab, tofacitinib, tumor necrosis factor inhibitor (TNFi) biosimilars, and biologic tapering/discontinuation, among others. We used the Grading of Recommendations, Assessment, Development and Evaluation methodology to assess the quality of evidence and formulate recommendations and required at least 70% agreement among the voting panel.ResultsRecommendations for AS and nonradiographic axial SpA are similar. TNFi are recommended over secukinumab or ixekizumab as the first biologic to be used. Secukinumab or ixekizumab is recommended over the use of a second TNFi in patients with primary nonresponse to the first TNFi. TNFi, secukinumab, and ixekizumab are favored over tofacitinib. Co‐administration of low‐dose methotrexate with TNFi is not recommended, nor is a strict treat‐to‐target strategy or discontinuation or tapering of biologics in patients with stable disease. Sulfasalazine is recommended only for persistent peripheral arthritis when TNFi are contraindicated. For patients with unclear disease activity, spine or pelvis magnetic resonance imaging could aid assessment. Routine monitoring of radiographic changes with serial spine radiographs is not recommended.ConclusionThese recommendations provide updated guidance regarding use of new medications and imaging of the axial skeleton in the management of AS and nonradiographic axial SpA.
An H∞non‐fragile observer‐based adaptive sliding mode controller design for uncertain fractional‐order nonlinear systems with time delay and input nonlinearity
Authors: Parvizian Majid , Khandani Khosro , Majd Vahid Johari 
Published: August 2019

AbstractIn this paper the problem of non‐fragile adaptive sliding mode observer design is addressed for a class of nonlinear fractional‐order time‐delay systems with uncertainties, external disturbance, exogenous noise, and input nonlinearity. An H∞ observer‐based adaptive sliding mode control considering the non‐fragility of the observer is proposed for this system. The sufficient asymptotic stability conditions are derived in the form of linear matrix inequalities. It is proven that the sliding surface is reachable in finite time. An illustrative example is provided which corroborates the effectiveness of the theoretical results.
Ion‐Selective Membrane‐Free Dual Sulfur‐Iodine Catholyte for Low‐Cost and High‐Power Flow Battery Applications
Authors: Huang Wei , Zou Qingli , Lu Yi‐Chun 
Published: August 2019

AbstractHighly concentrated polysulfide‐ (PS) and iodide‐based (I3−/I−) redox couples are promising active materials for redox flow battery applications owing to their high volumetric capacity. However, their applications in lithium redox flow batteries suffer from severe shuttle of iodine and PS and thus require the use of an ion‐selective ceramic membrane for stable operation. This inherent challenge critically limits the practical current density of the flow battery (<1 mA cm−2). Herein, we developed a dual PS‐LiI catholyte to simultaneously alleviate the shuttling of iodine and the passivation of Li2S, demonstrating stable ion‐selective membrane‐free Li‐catholyte cells achieving volumetric capacity between 30–60 Ah L−1catholyte with long cycle life (>150 cycles) at high current densities (3.8 mA cm−2 and 5.4 mA cm−2). The dual PS‐LiI catholyte not only increases the volumetric capacity and stability, but also removes the resistive and high‐cost ion‐selective membrane for low‐cost, high‐energy and high‐power flow battery applications.
Sam68 is required for the growth and survival of nonmelanoma skin cancer
Authors: Fu Kai , Sun Xin , Xia Xue , Hobbs Ryan P. , Guo Yajuan , Coulombe Pierre A. , Wan Fengyi 
Published: August 2019

AbstractAlthough targeting DNA repair signaling pathways has emerged as a promising therapeutic for skin cancer, the relevance of DNA damage responses (DDR) in the development and survival of nonmelanoma skin cancer (NMSC), the most common type of skin cancer, remains obscure. Here, we report that Src‐associated substrate during mitosis of 68 kDa (Sam68), an early signaling molecule in DDR, is elevated in skin tumor tissues derived from NMSC patients and skin lesions from Gli2‐transgenic mice. Downregulation of Sam68 impacts the growth and survival of human tumor keratinocytes and genetic ablation of Sam68 delays the onset of basal cell carcinomas (BCC) in Gli2‐transgenic mice. Moreover, Sam68 plays a critical role in DNA damage‐induced DNA repair and nuclear factor kappa B (NF‐κB) signaling pathways in keratinocytes, hence conferring keratinocyte sensitivity to DNA damaging agents. Together, our data reveal a novel function of Sam68 in regulating DDR in keratinocytes that is crucial for the growth and survival of NMSC.
Synthesis and Antibacterial Evaluation of Mannich Bases Derived from 1,2,4‐Triazole
Authors: Paneth Agata , Trotsko Nazar , Popiołek Łukasz , Grzegorczyk Agnieszka , Krzanowski Tomasz , Janowska Sara , Malm Anna , Wujec Monika 
Published: August 2019

AbstractThe series of novel Mannich bases were synthesized and evaluated for their in vitro antibacterial activity against Gram‐positive and Gram‐negative bacterial strains. The results showed that all compounds were less active than the drugs used as reference, but some of them had moderate potency against Staphylococcus epidermidis ATCC 12228 and Bacillus subtilis ATCC 6633. The presence of a phenyl ring in the position 4 of piperazine seems to be necessary for antibacterial activity in this class of compounds.
Collapse‐Resistant Large‐Sized 2D Metal‐Organic‐Framework‐Derived Nitrogen‐Doped Porous Ultrathin Carbon Nanosheets for High‐Performance Supercapacitors
Authors: Li Xiangyang , Zhou Jiangqi , Li Xin 
Published: August 2019

AbstractMetal‐organic‐framework (MOF)‐derived two‐dimensional materials have attracted tremendous attention in energy storage, because of their unique layered structure and abundant active sites. However, the facile synthesis of carbon materials simultaneously having large‐sized, ultrathin‐layered nanosheets and ultrahigh specific surface area remains a challenge. In this work, we develop a simple carbon protection strategy for efficiently stabilizing the ultrathin carbon nanosheets against structure degradation. The optimized as‐prepared sample (UPZCNs‐K4) exhibits a flat morphology with a large size (ca. 10 μm), a thickness of 2.8 nm, high pore volume, and a high capacitance of 402 F g−1 at 1 A g−1 in 6.0 M KOH aqueous electrolyte. An assembled symmetric supercapacitor delivers 16.18 W h kg−1 and 21.89 W h kg−1 output in KOH aqueous solution and Na2SO4 electrolytes, respectively. The excellent performances of the optimized sample can be attributed to the combination of ultrahigh specific surface area for ions storage and ultrathin structure with large size for a short ion diffusion distance. We believe this work will aid in designing ultrathin porous carbon nanosheets with new nanostructures toward energy‐related applications.
Evidence‐based statistical analysis and methods in biomedical research (SAMBR) checklists according to design features
Authors: Dwivedi Alok Kumar , Shukla Rakesh 
Published: August 2019

AbstractBackgroundStatistical analysis according to design features and objectives is essential to ensure the validity and reliability of the study findings and conclusions in biomedical research. Heterogeneity in reporting study design elements and conducting statistical analyses is often observed for the same study design and study objective in medical literatures. Sometimes, researchers face a lot of predicaments using appropriate statistical approaches highlighted by methodologists for a specific study design either due to lack of accessibility or understanding of statistical methods or unavailability of checklists related to design and analysis in a concise format. The purpose of this review is to provide the checklist of statistical analysis and methods in biomedical research (SAMBR) to applied researchers.Recent findingsWe initially identified the important steps of reporting design features that may influence the choice of statistical analysis in biomedical research and essential steps of data analysis of common studies. We subsequently searched for statistical approaches employed for each study design/study objective available in publications and other resources. Compilation of these steps produced SAMBR guidance document, which includes three parts. Applied researchers can use part (A) and part (B) of SAMBR to describe or evaluate research design features and quality of statistical analysis, respectively, in reviewing studies or designing protocols. Part (C) of SAMBR can be used to perform essential and preferred evidence‐based data analysis specific to study design and objective.ConclusionsWe believe that the statistical methods checklists may improve reporting of research design, standardize methodological practices, and promote consistent application of statistical approaches, thus improving the quality of research studies. The checklists do not enforce the use of suggested statistical methods but rather highlight and encourage to conduct the best statistical practices. There is a need to develop an interactive web‐based application of the checklists for users for its wide applications.
Triplet BODIPY and AzaBODIPY Derived Donor‐acceptor Dyads: Competitive Electron Transfer versus Intersystem Crossing upon Photoexcitation
Authors: Shao Shuai , Gobeze Habtom B. , Bandi Venugopal , Funk Christiane , Heine Brian , Duffy Maddie J. , Nesterov Vladimir , Karr Paul A. , D'Souza Francis 
Published: August 2019

AbstractThe bis‐iodo β‐pyrrole‐substituted BF2‐chelated dipyrromethene, I2BODIPY, and its structural analogue BF2‐chelated aza dipyrromethene, I2azaBODIPY, carrying a nitrogen at the meso‐position instead of carbon, were synthesized and characterized as new set of triplet sensitizers using different techniques. These sensitizers were further functionalized with fullerene, C60, at the central boron atom to build donor‐acceptor conjugates. Using spectral, electrochemical, and computational methods, these conjugates were characterized, and the energy levels were established. Intersystem crossing to populate the triplet state was observed upon excitation of I2BODIPY and I2azaBODIPY, however, the measured rates of kISC were found to be nearly two orders of magnitude higher for I2azaBODIPY (kISC∼1011 s−1) compared to I2BODIPY (kISC∼109 s−1). The energetics, kISC, and position of HOMO and LUMO levels was found to control the ability of the dyad to undergo electron transfer, although the donor‐acceptor distances were virtually the same in both I2BODIPY‐C60 and I2azaBODIPY‐C60 conjugates. Free‐energy calculations revealed that the photoinduced electron transfer process was thermodynamically feasible from only the singlet excited states in both conjugates. Consequently, electron transfer from the 1I2BODIPY* in competition with intersystem crossing was witnessed in the case of I2BODIPY‐C60 dyad while in the case of I2azaBODIPY‐C60 dyad, excitation of azaBODIPY led to a short‐lived charge transfer state involving the catechol bridge followed by populating the low‐lying 3I2azaBODIPY* state without promoting the process of charge separation involving C60. The lifetime of the charge‐separated states was in the ns range in the I2BODIPY‐C60 conjugate both in polar and nonpolar solvents.
The past, present, and future of coral heat stress studies
Authors: Cziesielski Maha J. , Schmidt‐Roach Sebastian , Aranda Manuel 
Published: August 2019

AbstractThe global loss and degradation of coral reefs, as a result of intensified frequency and severity of bleaching events, is a major concern. Evidence of heat stress affecting corals through loss of symbionts and consequent coral bleaching was first reported in the 1930s. However, it was not until the 1998 major global bleaching event that the urgency for heat stress studies became internationally recognized. Current efforts focus not only on examining the consequences of heat stress on corals but also on finding strategies to potentially improve thermal tolerance and aid coral reefs survival in future climate scenarios. Although initial studies were limited in comparison with modern technological tools, they provided the foundation for many of today's research methods and hypotheses. Technological advancements are providing new research prospects at a rapid pace. Understanding how coral heat stress studies have evolved is important for the critical assessment of their progress. This review summarizes the development of the field to date and assesses avenues for future research.
High cell density transient transfection of CHO cells for TGF‐β1 expression
Authors: Elshereef Abdalla A. , Jochums André , Lavrentieva Antonina , Stuckenberg Lena , Scheper Thomas , Solle Dörte 
Published: August 2019

AbstractHigh cell densities for transient transfection with polyethyleneimine (PEI) can be used for rapid and maximal production of recombinant proteins. High cell densities can be obtained by different cultivation systems, such as batch or perfusion systems. Herein, densities up to 18 million cells/mL were obtained by centrifugation for transfection evaluation. PEI transfection efficiency was easily determined by transfected enhanced green fluorescence protein (EGFP) reporter plasmid DNA (pDNA). A linear correlation between fluorescence intensity and transfection efficiency was improved. The transfection efficiency of PEI was highly dependent on the transfection conditions and directly related to the level of recombinant protein. Several factors were required to optimize the transient transfection process; these factors included the media type (which is compatible with low or high cell density transfection), the preculture CHO‐K1 suspension cell density, and the pDNA to PEI level. Based on design of experiment (DoE) analyses, the optimal transfection conditions for 10 × 106 cells/mL in the CHOMACS CD medium achieved 73% transfection efficiency and a cell viability of over 80%. These results were confirmed for the production of transforming growth factor‐beta 1 (TGF‐β1) in a shake flask. The purified TGF‐β1 protein concentration from 60 mL supernatant was 27 µg/mL, and the protein was biologically active.