bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arginine to glutamine mutation in olfactomedin like 3 (OLFML3) is a candidate for severe goniodysgenesis and glaucoma in the Border Collie dog breed
Authors: Pugh Carys A , Farrell Lindsay L , Carlisle Ailsa J , Bush Stephen J , Trejo-Reveles Violeta , Matika Oswald , de Kloet Arne , Walsh Caitlin , Bishop Stephen C , Prendergast James GD , Schoenebeck Jeffrey J , Rainger Joe , Summers Kim M 
Published: May 2018

AbstractGoniodysgenesis is a developmental abnormality of the anterior chamber of the eye. It is generally considered to be congenital in dogs (Canis lupus familiaris), and has been associated with glaucoma and blindness. Goniodysgenesis and early-onset glaucoma initially emerged in Border Collies in Australia in the late 1990s and has subsequently been found in Europe and the USA. The objective of the present study was to determine the genetic basis of goniodysgenesis in Border Collies. Clinical diagnosis was based on results of examinations by veterinary ophthalmologists of affected and unaffected dogs from eleven different countries. Genotyping using the Illumina high density canine SNP chip and whole genome sequencing were used to identify candidate genetic regions. Expression profiles and evolutionary conservation of candidate genes were assessed using public databases. Analysis of pedigree information was consistent with an autosomal recessive mode of inheritance for severe goniodysgenesis (potentially leading to glaucoma) in this breed. There was a highly significant peak of association over chromosome 17, with ap-value of 2 × 10-13. Whole genome sequences of three dogs with glaucoma, three severely affected and three unaffected dogs identified a missense variant in the olfactomedin like 3 (OLFML3) gene in all six affected animals. This was homozygous in all nine cases with glaucoma and 12 of 14 other severely affected animals. Of 67 reportedly unaffected animals, only one (offspring of two homozygous affected parents) was homozygous for this variant. The identification of a candidate genetic region and putative causative mutation will aid breeders to reduce the frequency of goniodysgenesis and the risk of glaucoma in the Border Collie population.
Support for the dominance theory inDrosophilatranscriptomes
Authors: Llopart Ana , Brud Evgeny , Pettie Nikale , Comeron Josep M. 
Published: May 2018

ABSTRACTInteractions among divergent elements of transcriptional networks from different species can lead to misexpression in hybrids through regulatory incompatibilities, some with the potential to generate sterility. Genes with male-biased expression tend to be overrepresented among genes misexpressed in hybrid males. While the possible contribution of faster-male evolution to this misexpression has been explored, the role of the hemizygousXchromosome (i.e., the dominance theory for transcriptomes) remains yet to be determined. Here we study genome-wide patterns of gene expression in females and males ofDrosophila yakubaandD. santomeaand their hybrids. We used attached-X stocks to specifically test the dominance theory, and we uncovered a significant contribution of recessive alleles on theXchromosome to hybrid misexpression. Our analysis of gene expression patterns suggests that there is a contribution of weakly deleterious regulatory mutations to gene expression divergence in the sex towards which the expression is biased. In the opposite sex (e.g., genes with female-biased expression analyzed in male transcriptomes), we detect stronger selective constraints on gene expression divergence. Although genes with high degree of male-biased expression show a clear signal of faster-X evolution for gene expression divergence, we also detected slower-X evolution of gene expression in other gene classes (e.g. female-biased genes) that is mediated by significant decreases ofcis- andtrans-regulatory divergence. The distinct behavior of X-linked genes with high degree of male-biased expression is consistent with these genes experiencing a higher incidence of positively selected regulatory mutations than their autosomal counterparts. We propose that both dominance theory and faster-X evolution of gene expression may be major contributors to hybrid misexpression and possibly the large X-effect in these species.
Assessment of urinary pharmacodynamic profiles of faropenem against extended-spectrum β-lactamase-producing Escherichia coli with canine ex vivo modeling
Authors: Harada Kazuki , Shimizu Takae , Miyashita Naoki , Hikasa Yoshiaki 
Published: May 2018

AbstractExtended-spectrum β-lactamase (ESBL)-producing bacteria are of great concern in companion animals with urinary tract infections (UTIs). Because of its high safety and stability in the presence of ESBLs, faropenem is assumed to be a candidate antimicrobial agent for canine UTIs with ESBL-producing bacteria. This study was performed to investigate the urinary pharmacokinetics and pharmacodynamics of faropenem administered at 5 mg/kg body weight in six healthy dogs using an ex vivo model. Six UTI pathogenic strains of ESBL-producing Escherichia coli (ESBL-EC) with the following faropenem minimum inhibitory concentrations (MICs) were used: 1 µg/mL (n = 2), 2 µg/mL (n = 2), 4 µg/mL (n = 1), and 16 µg/mL (n = 1). Urine samples were obtained every 4 h for the first 12 h after faropenem administration for measurement of the urine drug concentration and urinary bactericidal titers (UBTs). The urine concentration of faropenem peaked at 0 to 4 h after administration, with a mean maximum concentration of 584 μg/mL, and markedly decreased at 8 to 12 h (23 μg/mL). The median UBTs for all tested ESBL-EC strains were highest at 0 to 4 h and then significantly decreased at 8 to 12 h. These findings indicate that administration of faropenem more than once daily is recommended for the treatment of ESBL-EC-related UTIs in dogs. In addition, the median areas under the UBT–time curves (AUBTs) were significantly inversely correlated with the corresponding MICs for faropenem in the tested strains (P < 0.05). Notably, the median AUBTs were significantly higher in ESBL-EC strains with an MIC of 1 µg/mL than in those with an MIC of ≥4 µg/mL (P < 0.05). The present study serves as the basis of clinical application of faropenem for ESBL-producing bacteria-related UTIs in dogs.
The metastable XBP1u transmembrane domain defines determinants for intramembrane proteolysis by signal peptide peptidase
Authors: Yücel Sara Suna , Stelzer Walter , Lorenzoni Alessandra , Wozny Manfred , Langosch Dieter , Lemberg Marius K. 
Published: May 2018

SummaryUnsplicedXBP1mRNA encodes XBP1u, the transcriptionally inert variant of the unfolded protein response (UPR) transcription factor XBP1s. XBP1u targets its mRNA-ribosome-nascent-chain-complex to the endoplasmic reticulum (ER) to facilitate UPR activation and prevents overactivation. Yet, its membrane association is controversial. Here, we use cell-free translocation and cellular assays to define a moderately hydrophobic stretch in XBP1u that is sufficient to mediate insertion into the ER membrane. Mutagenesis of this transmembrane (TM) region reveals residues that facilitate XBP1u turnover by an ER-associated degradation route that is dependent on signal peptide peptidase (SPP). Furthermore, the impact of these mutations on TM helix dynamics was assessed by residue-specific amide exchange kinetics, evaluated by a semi-automated algorithm. Based on our results, we suggest that SPP-catalyzed intramembrane proteolysis of TM helices is not only determined by their conformational flexibility, but also by side chain interactions near the scissile peptide bond with the enzyme’s active site.
Relief of the Dma1-mediated checkpoint requires Dma1 autoubiquitination and dynamic localization
Authors: Jones Christine M. , Chen Jun-Song , Johnson Alyssa E. , Elmore Zachary C. , Cullati Sierra N. , Beckley Janel R. , Gould Kathleen L. 
Published: May 2018

AbstractChromosome segregation and cell division are coupled to prevent aneuploidy and cell death. In the fission yeastSchizosaccharomyces pombe, the septation initiation network (SIN) promotes cytokinesis, but upon mitotic checkpoint activation, the SIN is actively inhibited to prevent cytokinesis from occurring before chromosomes have safely segregated. SIN inhibition during the mitotic checkpoint is mediated by the E3 ubiquitin ligase Dma1. Dma1 binds to the CK1-phosphorylated SIN scaffold protein, Sid4, at the SPB, and ubiquitinates it. Sid4 ubiquitination antagonizes the SPB localization of the Polo-like kinase Plo1, the major SIN activator, so that SIN signaling is delayed. How this checkpoint is silenced once spindle defects are resolved has not been clear. Here we establish that Dma1 transiently leaves SPBs during anaphase B due to extensive auto-ubiquitination. The SIN is required for Dma1 to return to SPBs later in anaphase. Blocking Dma1 removal from SPBs by permanently tethering it to Sid4 prevents SIN activation and cytokinesis. Therefore, controlling Dma1’s SPB dynamics in anaphase is an essential step in S.pombecell division and the silencing of the Dma1-dependent mitotic checkpoint.
Information creates relative bargaining power in vendor negotiations
Authors: Macy Katharine V. 
Published: May 2018

Purpose
This paper aims to examine how libraries can create relative bargaining power and presents a methodology for analyzing collections and preparing for negotiations.

Design/methodology/approach
A brief literature review of the current state of collection budgets and electronic resource prices is presented prior to proposing a methodology based on business analysis frameworks and techniques.

Findings
Electronic resource subscription prices are increasing at a rate significantly higher than inflation, while collection budgets grow slowly, remain stagnant or decrease. Academic libraries have the ability to counteract this trend by creating relative bargaining power through organizational efforts that take advantage of size and concentration (e.g. consortia), vertical integration through practices such as library publishing and open access and through individual efforts using information. This paper proposes metrics and methodologies that librarians can use to analyze their collections, set negotiation priorities and prepare for individual resource negotiations to create relative bargaining power.

Practical implications
The proposed methodology enables librarians and buyers of information resources to harness the information available about their electronic resource collections to better position themselves when entering negotiations with vendors.

Originality/value
This paper presents metrics, some not commonly used (i.e. average annual price increase/decrease), that aid in understanding price sensitivity. Pareto analysis has been traditionally used to analyze usage, but this paper suggests using it in relation to costs and budgets for setting negotiation priorities.
Work in progress: the progression of competence-based employability
Authors: Froehlich Dominik Emanuel , Liu Mingyang , Maria Van der Heijden Beatrice Isabella Johanna 
Published: May 2018

PurposeEmployability and its components have received a lot of attention from scholars and practitioners. However, little is known about the interrelations between these different components of employability and how employees progress within their employability trajectories. Therefore, a model of such progression was constructed and tested using Van der Heijde and Van der Heijden’s (2006) employability measurement instrument. The paper aims to discuss these issues.Design/methodology/approachThe propositions were tested empirically by applying a Rasch model using a sample of 167 Austrian business consultants.FindingsThe findings lend some support for the hypothesized progression model of employability. Specifically, the items measuring occupational expertise are largely located in the group of items that were relatively likely to be endorsed. Also, the items of personal flexibility and anticipation and optimization were, in general, less likely to be endorsed than the items of occupational expertise.Research limitations/implicationsThe major thrust of this paper is a theoretical one. However, the empirical demonstration tentatively supports the proposed model, which implies that further, more robust longitudinal research in this direction may be a worthwhile endeavor.Practical implicationsBy understanding which competences are important at which stage or across which stages of an individual’s career, career advisors and human resource management professionals can give more targeted advice concerning career management practices.Originality/valueThe present study contributes to the literature by investigating how employees may make progress within their employability trajectories.
My 28-year journey with cannabis: from terminal disease to post-pharmaceutical healing
Authors: Godfrey Sarah Jane 
Published: May 2018

Purpose
The purpose of this paper is to convey the experience of medical cannabis users and growers in the UK.

Design/methodology/approach
Biography and personal ethnograpy.

Findings
Medical cannabis users are forced into cultivating their own medicine.

Research limitations/implications
Single case study.

Practical implications
There is an urgent need for policy change to enable medical cannabis users to access their medication easily and affordably.

Social implications
A rising number of people are denied their constitutional right to health by a misguided policy.

Originality/value
This study fills a major gap in the literature on medical cannabis growers.
International competitiveness and ex-ante treatment effects of CPEC on household welfare in Pakistan
Authors: Naz Lubna , Ali Adeel , Fatima Ambreen 
Published: May 2018

PurposeThis paper aims to presents one of the first direct micro-econometric impact of competitive industries (based on revealed comparative advantage [RCA] between Pakistan and China) on household welfare in Pakistan using semi-parametric matching technique.Design/methodology/approachThe study has also measured and identified the industrial competitiveness in both agricultural and non-agricultural (manufacturing) industries using RCA approach. RCA at the four-digit ISIC level are matched to household survey data (Pakistan Social and Living Standard Measurement) for 2013-2014 to represent the competitive industries in which the household’s higher earner is employed.FindingsThe findings of the study reveal that the China–Pakistan ex-post treatment effect (industrial competitiveness) provides welfare-improving effects. Furthermore, on this behalf, this study further assesses ex-ante treatment effects of recently signed China–Pakistan Economic Corridor (CPEC) on household welfare and suggests that CPEC would boost further trade liberalization and, therefore, would lead to industrial competitiveness and hence economic growth.Originality/valuePaper contributes to two streams of literature. First, it measures and identifies the industrial competitiveness in both agricultural and non-agricultural industries using RCA approach; and second, it assesses the welfare of those households associated with these industries using semi-parametric propensity score matching technique.
Vulnerable employment in Mauritius: experience of an upper-middle-income country
Authors: Gokhool Suneila , Kasseeah Harshana , Tandrayen-Ragoobur Verena 
Published: May 2018

Purpose
The purpose of this paper is to shed light on the socio-economic characteristics of workers engaged in vulnerable jobs in Mauritius. The study has a particular focus on the gender and youth dimensions of vulnerable employment. The study also provides a pre-crisis and post-crisis analysis of vulnerable employment.

Design/methodology/approach
The paper uses several waves of the continuous multi-purpose household survey, which is a high-quality individual-level data set, to study vulnerable employment. Several definitions of vulnerable employment are used to identify the workers employed in vulnerable jobs. These include “own-account” workers and “contributing family workers”.

Findings
The results obtained suggest that women and young workers have a lower probability of being in vulnerable employment. Marital status, age and education are also important variables influencing the probability of being in vulnerable employment.

Research limitations/implications
The paper has important policy implications regarding welfare and education policies. Appropriate mechanisms need to be put in place for the social protection and training of workers so that they do not end up in vulnerable jobs.

Originality/value
This paper studies Mauritius as it is a small island economy vulnerable to external shocks. Vulnerable unemployment has often been understudied as the focus of many studies has been solely on employment, and the quality of employment has often not been considered.
Skill drain from ASEAN countries: can sending countries afford?
Authors: Ullah AKM Ahsan 
Published: May 2018

PurposeMigration of skilled workers to other countries remains a highly contentious issue. Skill drain does not take place based on skill surplus and deficient equation. Skilled migrants can make their choice to migrate on their own with minimal control of the Government. This paper aims to argue that sending countries lose skill which cannot be offset or justified by the remittances inflow.Design/methodology/approachThis paper is based on a research conducted on skill migration from the Philippines, Singapore and Thailand. In this study, skilled migrants are engineers, medical doctors, nurses and academics. The author interviewed 12 engineers (four from the Philippines; two from Malaysia; four from Singapore and two from Thailand); nine medical doctors (four from the Philippines; three from Singapore, one from Malaysia and one from Thailand); eight nurses (six from the Philippines and two from Thailand); and 14 academics (six from the Philippines; five from Singapore and three from Malaysia) who were working abroad.FindingsSkill migration continues to grow because of the growing demand, wage differentials, glorifications of the contribution of remittances to development and failure of the origin countries to retain them. The question remains whether the respective sending country is producing more of them so that they can send after their own demand is met. This paper investigates whether the sending end can afford exporting such skills.Originality/valueThis is an important contribution to the scholarship.
Relationship between sustainability and risk management in fashion supply chains
Authors: Rafi-Ul-Shan Piyya Muhammad , Grant David B. , Perry Patsy , Ahmed Shehzad 
Published: May 2018

Purpose
Fashion supply chain (FSC) research has identified two important issues of sustainability management and risk management. However, investigation of these issues is relatively sparse and has primarily been independent with little combinatory research, despite their important interrelationships. The purpose of this paper is to address that gap by critically reviewing extant literature to synthesise important sustainability risk issues in FSCs and proposing an empirical research agenda.

Design/methodology/approach
This paper uses a structured literature review approach and Denyer and Tranfield’s (2009) context, intervention, mechanisms and outcome (CIMO) criteria for critical analysis to enable the development of future empirical research areas.

Findings
While sustainability and risk are discussed independently in the supply chain literature, combinatory discussions are very limited, despite the interdependence of these concepts. There is little substantial research on sustainability risk in global FSCs and therefore, an empirical research agenda is proposed with the four research directions to address the gap and take forward the notion of supply chain sustainability risk management in FSCs: definition; organisation and management; influence on performance; and development of a conceptual framework.

Research limitations/implications
This paper provides a critical literature review and thus lacks empirical study.

Practical implications
This paper highlights important issues in sustainability risk management for FSCs and presents an agenda for future empirical research.

Originality/value
This paper contributes by providing a combinatory synthesis of sustainability and risk management in FSC literature and an agenda for future empirical research.
Tertiary-level internet users’ opinions and perceptions of cyberhate
Authors: Celik Serkan 
Published: May 2018

Purpose
The purpose of this paper is to elaborate the opinions and perceptions of internet users regarding online hate speech, and bring cyberhate to the attention of internet users and policy stakeholders.

Design/methodology/approach
A sectional research design and survey method was adopted throughout the study to examine the opinions and perceptions of internet users regarding cyberhate by descriptively exploring the existing situation from various perspectives. The participants of the study were determined by purposive sampling methods to attain maximum variety among internet users who are considered as highly literate in technology use. The data were collected through a personal data form and a survey (Cyberhate Perception Scale) from 372 internet users living in Turkey and the USA.

Findings
The findings of the study revealed that the majority of participants do not perceive cyberhate as a part of freedom of speech and they believe that online hate behaviors, which they also consider to be a violation of human rights, should be deemed illegal and be punished accordingly. The findings, which were discussed in line with the existing research, indicated some significant predictors of internet users’ perceptions on cyberhate.

Originality/value
As cyberhate is an understudied area that raises concerns in terms of internet user exposure, the objective of this research is to understand tendencies about the opinions and perceptions of internet users regarding online hate speech, and bring cyberhate to the attention of internet users and policy stakeholders.