bluuebunny/crossref_metadata_2025_split huggingface dataset:
Casuística do triênio 2013-2015 de atuação do laboratório de Patologia Veterinária
Authors: Mendes Ricardo Evandro , Rhoden Leandro Anderson , Casagrande Renata Assis , Henker Luan Cleber , Schwertz Claiton Ismael , Piva Manoela Marchezan , Stedille Fernanda Agustini , Gabriel Mateus Eloir , Christ Ricardo 
Published: March 2019

Este trabalho apresenta a casuística de diagnósticos nos anos de 2013 a 2015 do Laboratório de Patologia Veterinária do IFC Campus Concórdia. Foram 399 diagnósticos em bovinos, 68 em ovinos e 149 em suínos, totalizando 616. Destes, 505 (78,7%) foram através de necropsias e 131 (21,3%) através de amostras formolizadas enviadas por veterinários. Em bovinos as doenças mais prevalentes foram babesiose com 16 casos (4,0%); leucose enzoótica bovina com 15 casos (3,8%); endocartite e peritonite com 14 casos cada (3,5%); intoxicação por Pteridium arachnoideum e hemoncose com 13 casos cada (3,3%). Já em ovinos hemoncose com 17 casos (25,0%) e suínos 13 casos (8,7%) de circovirose, 12 casos (8,0%) de doença de Glässer e 12 casos (8,0%) de meningite supurativa.
Deficiência de Selênio como causa de mortalidade em leitões neonatos no Oeste de Santa Catarina
Authors: Mendes Ricardo Evandro , Gabriel Mateus Eloir , Da Rosa Rafael Alves 
Published: March 2019

Dentre as alterações clínicas observadas em casos de deficiência de selênio e vitamina E, destaca-se a hepatose dietética em suínos, acometendo geralmente leitões jovens e de crescimento rápido. São escassos os relatos acometendo leitões neonatos. Este trabalho relata três surtos de mortalidade por hepatose dietética em leitões com poucos dias de vida, em granjas do Oeste de Santa Catarina, Brasil, descrevendo as alterações clínico-patológicas encontradas. Os níveis de selênio hepático dos leitões do terceiro surto foram mensurados e encontravam-se cerca de 11 vezes abaixo dos níveis considerados ideais. Determinou-se a quantidade de vitamina E e selênio na ração fornecida às matrizes da granja, sendo que estas estavam de acordo com o recomendado. Sugere-se, então, que a causa da deficiência de selênio, níveis deste mineral nas matrizes, em decorrência a um excesso de ácidos graxos poliinsaturados na alimentação destas fêmeas.Palavras chave: Hepatose dietética, suínos, ácidos graxos poliinsaturados.
Casuística de neoplasias diagnosticadas em bovinos do Oeste de Santa Catarina - um estudo retrospectivo
Authors: Mendes Ricardo Evandro , Stedille Fernanda Agustini , Pozzo Ildo Dal , Broll Fabrisio 
Published: March 2019

As neoplasias fazem parte dos diagnósticos estabelecidos em bovinos, a partir de necropsias e fragmentos enviados para análise ao Laboratório de Patologia Veterinária do Instituto Federal Catarinense Campus Concórdia (LPV – IFC). O presente estudo teve por objetivo identificar a prevalência de neoplasias em bovinos no período dos três primeiros anos (2013 a 2015) de atuação do laboratório na região Oeste Catarinense. Realizou-se um levantamento de dados nos arquivos, onde foram identificados 46 diagnósticos de neoplasias, correspondendo a 11,4%, dentre um total de 402 bovinos avaliados. Identificou-se ainda, prevalência por raça, sexo, idade e localização anatômica.As neoplasias mais frequentes foram linfossarcoma (32,6%) e o carcinoma de células escamosas (28,1%). Concluiu-se que as neoplasias são uma importante causa de mortalidade e possível fator que leva ao descarte de bovinos, pois representaram um expressivo número dos diagnósticos em bovinos no período de tempo avaliado (3 anos). Em virtude do predomínio da etiologia viral do linfossarcoma e da intoxicação crônica pelo Pteridium arachnoideum no carcinoma de células escamosas, medidas de prevenção e controle deveriam ser instauradas na região para reduzir os prejuízos econômicos.Palavras-chave: Bovinos, neoplasias, diagnóstico.
INTOXICAÇÃO POR ÁCIDO CIANÍDRICO NO ALTO URUGUAI CATARINENSE - ESTUDO RETROSPECTIVO (2013-2017)
Authors: Broll Fabrisio , Bavaresco Lucas H. , Piva Manoela M. , Carneiro Daniele C. S. , Gris Anderson , Cechin Renan A. , Werlang Rafael A. , Gimeses Isabela , Chiocca Raul , Gomes Teane M. A. , Mendes Ricardo E. 
Published: March 2019

São consideradas plantas cianogênicas aquelas que contêm como princípio tóxico o ácido cianídrico (HCN). Esta é uma substância muito volátil e considerada como uma das mais tóxicas conhecidamente. Este trabalho teve como objetivo relatar a ocorrência dos casos de intoxicação por ácido cianídrico em bovinos no Alto Uruguai Catarinense, diagnosticados pelo Laboratório de Patologia V eterinária (LPV) do Instituto Federal Catarinense – Campus Concórdia. Para isso, realizou-se um estudo retrospectivo dos diagnósticos em bovinos entre 2013 e 2017. Neste período, foram realizadas 641 necropsias em bovinos, sendo que oito foram devido à intoxicações por ácido cianídrico, correspondendo a 1,25%. A partir dos dados coletados, observou-se uma baixa, mas importante ocorrência desse tipo de intoxicação nos animais desta região. É necessária a continuidade nos trabalhos de divulgação e diagnóstico por parte do laboratório e dos profissionais envolvidos com a cadeia bovina da região, para que essas perdas econômicas sejam minimizadas.Palavras-chave: bovino, patologia, pessegueiro bravo, diagnóstico, necropsia.
INTOXICAÇÃO POR NITRATO/NITRITO NO OESTE DE SANTA CATARINA - ESTUDO RETROSPECTIVO (2013-2017)
Authors: Neto Eduardo P. , Mingotti Taisson R. , Gris Anderson , Cechin Renan A. , Christ Ricardo , Perosa Fernanda F. , Werlang Rafael A. , Braga Francisco J. , Gomes Teane A. M. , Casagrande Renata A. , Mendes Ricardo E. 
Published: March 2019

A intoxicação por nitrato/nitrito é uma enfermidade comum no estado de Santa Catarina, e que leva a morte súbita em bovinos. Trata-se de um distúrbio ocasionado pela utilização excessiva de adubação nitrogenada nas pastagens e condições climáticas adversas, como diminuição de luminosidade ou déficit hídrico, levando ao acúmulo de nitrato na pastagem. Este trabalho teve como objetivo relatar a ocorrência dos casos de intoxicação por nitrato/nitrito em bovinos na região oeste de Santa Catarina, diagnosticados pelo Laboratório de Patologia Veterinária (LPV) do Instituto Federal Catarinense – Campus Concórdia. Entre os anos de 2013 e 2017, foram diagnosticados por necropsia 10 surtos de intoxicações por nitrato/nitrito. De um total de 641 bovinos necropsiados pelo LPV nesse período, 19 correspondem a essa intoxicação, representando 2,96% da casuística de necropsias e 2,20% da casuística total de diagnósticos (necropsias e histopatológico). Ainda que seja um número expressivo de causa mortis, acredita-se que existam muitos outros casos não diagnosticados pelo LPV, visto que nem todos os animais são remetidos para necropsia. Conclui-se que as intoxicações por nitrato/nitrito são expressiva causa de morte nos rebanhos bovinos da região, devendo serem adotadas medidas de controle e prevenção. Palavras-chave: metemoglobina, patologia, bovino, diagnóstico, necropsia.
INTOXICAÇÃO POR PTERIDIUM ARACHNOIDEUM NO OESTE DE SANTA CATARINA - ESTUDO RETROSPECTIVO (2013-2017)
Authors: Mingotti Taisson R. , Alves Felipe D. , Gris Anderson , Cechin Renan A. , Perosa Fernanda F. , Bavaresco Lucas H. , Werlang Rafael A. , Lima Jéssica L. F. de , Gomes Teane M. A. , Casagrande Renata A. , Mendes Ricardo E. 
Published: March 2019

Samambaia (Pteridium arachnoideum) é uma das plantas tóxicas mais importantes na criação de bovinos de todo o Brasil. O objetivo desse trabalho foi descrever os diagnósticos de intoxicação por samambaia, em bovinos na região oeste de Santa Catarina, realizados pelo Laboratório de Patologia Veterinária (LPV) do IFC – Campus Concórdia, no período entre 2013 e 2017. No período, foram realizadas 641 necropsias de bovinos, sendo que 22 dessas tiveram o diagnóstico de intoxicação por samambaia, totalizando 3,4% da casuística total de necropsias em bovinos. Foram 13 casos de síndrome hemorrágica aguda, seis casos de carcinomas de células escamosas na base da língua, esôfago ou rúmen, e três casos de hematúria enzoótica bovina. Apesar dos inúmeros trabalhos científicos a respeito da toxicidade do P. arachnoideum, o número de diagnósticos dessa intoxicação é alto na região, e a planta ainda é facilmente encontrada. A melhor forma de diminuir as intoxicações é o adequado manejo das pastagens e a correta adubação do solo, evitando com que a planta esteja disponível ao consumo dos animais, porém essa informação precisa ser difundida aos produtores rurais. Palavras-chave: diagnóstico, patologia, samambaia, hematúria, carcinoma.
Arthritis prevention in the pre-clinical phase of RA with abatacept (the APIPPRA study): a multi-centre, randomised, double blind, parallel group placebo-controlled clinical trial Protocol
Authors: Al-Laith Mariam , Jasenecova Marianna , Abraham Sonya , Bosworth Aisla , Bruce Ian N. , Buckley Christopher D. , Ciurtin Coziana , D’Agostino Maria-Antonietta , Emery Paul , Gaston Hill , Isaacs John D. , Filer Andrew , Fisher Benjamin A. , Huizinga Thomas W. J. , Ho Pauline , Jacklin Clare , Lempp Heidi , McInnes Iain B. , Pratt Arthur G. , Östor Andrew , Raza Karim , Taylor Peter C. , Schaardenburg Dirkjan van , Shivapatham Dharshene , Wright Alison J. , Vasconcelos Joana C. , Kelly Joanna , Murphy Caroline , Prevost A. Toby , Cope Andrew P. 
Published: March 2019

Abstract
Trial design: We present a study protocol for a multi-centre, randomised, double blind, parallel group, placebo-controlled trial that seeks to test the feasibility, acceptability and effectiveness of a 52-week period of treatment with the first-in-class costimulatory blocker abatacept for preventing or delaying the onset of inflammatory arthritis. Methods: The study aimed to recruit 206 male or female subjects from the secondary care hospital setting across the UK and the Netherlands. Participants aged 18 or over who report inflammatory sounding joint pain (clinically suspicious arthralgia) and who are found to be positive for serum autoantibodies associated with RA were eligible for enrolment. All study subjects were randomised to receive weekly injections of investigational medicinal product (IMP), either abatacept or placebo treatment over a 52-week period. Participants were then followed up for a further 52 weeks. The primary endpoint was defined as the time to development of ≥ 3 swollen joints, or to the fulfilment of the 2010 ACR/EULAR classification criteria for RA using swollen but not tender joints, whichever endpoint was met first. In either case, swollen joints were confirmed by ultrasonography. Participants, care givers, and those assessing the outcomes were all blinded to group assignment. Clinical assessors and ultrasonographers were also blinded to each other’s assessments for the duration of the study. Conclusions: There is limited experience of the design and implementation of trials for the prevention of inflammatory joint diseases. We discuss the rationale behind choice and duration of treatment, the challenges associated with defining the “at risk” state, and offer pragmatic solutions in the protocol to enrolling subjects at risk of RA. Trial registration: Current Controlled Trials, ID: ISRCTN46017566. Registered on 04 July 2014.
Assessment of the Deformation of the Outer Nuclear Layer in the Epiretinal Membrane Using Spectral-Domain Optical Coherence Tomography
Authors: Takagi Seiji , Kudo Shigeki , Yokota Hideo , Akiba Masahiro , Mandai Michiko , Hirami Yasuhiko , Takahashi Masayo , Kurimoto Yasuo , Ishida Masahiro 
Published: March 2019

Abstract
Background

We aimed to investigate the deformation of the outer nuclear layer using optical coherence tomography in patients with epiretinal membrane (ERM) and its relationship with metamorphopsia.

Methods

Thirty-nine eyes from 39 patients with ERM were included in the study. Patients with the subtypes of pseudo macula hole and lamellar hole were excluded. Twenty-one fellow eyes without macular disease were included as normal controls. Forty-nine B-scan images were obtained in the range of 20 degrees around the macula using SD-OCT. The outer nuclear layer (ONL) was evaluated as a three-dimensional image (3D-ONL) reconstructed using the distance between the ONL and retinal pigment epithelium (RPE) line. The deformation of the ONL was figured at the reference plane and evaluation plane (ONL-B). The characteristic parameters of the ONL-B were defined as circularity, area ratio, and axis ratio. The correlations between these parameters and visual acuity and MCHART score ratio (MH/MV) were then evaluated.

Results

ONL height was significantly higher in ERM patients than in normal controls (54.1 ± 5.3 μm and 84.1 ± 12.9 μm, respectively; P<0.001). In ERM patients, the MV score was 0.53 ± 0.50, the MH score was 0.71 ± 0.61, and the distance from the RPE line to the ONL-B was 153.5 ±13.5µm. The axis of the ONL-B in normal controls and ERM patients was -6.25±21.8 and -1.28±29.1, respectively, which indicates that the ONL is horizontally long in both normal individuals and ERM patients. The circularity and area ratio were significantly smaller in ERM patients than in normal controls. In all ERM patients, MH/MV had a significant correlation with axis (r=-0.29, p=0.034), circularity (r=-0.28, p=0.044), area ratio (r=-0.47, p=0.001). Moreover, we found that the correlation was more significant if the subjects had an axis of the ONL within ± 10 degrees (n=16); the correlations of MH/MV with axis (r=-0.29, p=0.034), circularity (r=-0.53, p=0.021), and area ratio were more significant (r=-0.78, P<0.0001).

Conclusion

The ONL is horizontally long in normal individuals and ERM patients. The direction of metamorphopsia is correlated with the direction of ONL deformation.
Cognitive behavioural therapy augmented with virtual reality exposure for treatment of social anxiety: A randomised clinical trial
Authors: Arnfred Benjamin , Bang Peter , Hjorthøj Carsten , Winding Clas , Møller Kirsten , Hvenegaard Morten , Gausbøl Ulrik Krog , Sø Ditte , Rosenberg Nicole , Nordentoft Merete 
Published: March 2019

Abstract
Background

Social Anxiety Disorder (SAD) has high lifetime prevalence, early onset and long duration or chronicity. Cognitive Behavioural Therapy (CBT) has best evidence for treatment effect and planned exposure is considered one of the most effective elements of CBT in SAD. However, in vivo exposure can be difficult to access and control and is sometimes rejected by patients because they expect it to be too aversive. The use of virtual reality allows exposure to challenging situations in an immersive, but also protected, flexible and controlled environment.

Methods

The design is an investigator initiated randomized, assessor-blinded, parallel group and superiority designed clinical trial. 302 patients diagnosed with social anxiety disorder, will be included from two psychotherapeutic outpatient clinics at Mental Health Center Copenhagen. All patients will be offered a manual-based 14-week CBT group program, including eight sessions of exposure therapy. Patients will be randomised with concealed allocation sequence to CBT augmented with virtual reality exposure or CBT. Patients will be assessed at baseline, post treatment and at one-year follow-up by researchers blinded for treatment condition. The primary outcome will be social anxiety measured with Liebowitz Social Anxiety Scale. Secondary outcome measures will be depression, social functioning, and patient satisfaction. Exploratory outcomes will be substance and alcohol use, working alliance and quality of life.

Discussion

The SO REAL trial will be the hitherto largest trial investigating the use of virtual reality as augmentation of CBT and the results may guide future clinical treatments.

Trials registration
The project was registered at clinicaltrials.gov on 02/19/2019 as NCT03845101. Can be found online at: https://clinicaltrials.gov/ct2/show/NCT03845101
Radiotherapy versus radiotherapy combined with temozolomide in high-risk low-grade gliomas after surgery:Study protocol for a randomized controlled clinical trial
Authors: Wang Jingjing , Peng Xingchen , He Yan , Guan Hui , Mu Xiaoli , He Ling , Wang Ying 
Published: March 2019

Abstract
Abstract

Background
It has been reported that radiation therapy followed by PCV chemotherapy (procarbazine, lomustine and vincristine) could improve progression-free survival (PFS) and overall survival (OS) in patients with high-risk WHO grade 2 gliomas after surgery. However, procarbazine is not available in China. In clinical practice, Chinese doctors often use radiotherapy combined with temozolomide to treat these patients, though large-scale prospective studies are lacking. This trial aims to confirm whether RT combined with temozolomide (TMZ) can improve PFS and OS in patients with high-risk low-grade gliomas (LGGs).

Methods/design
This is a two-group, randomized controlled trial (RCT) enrolling patients who have low-grade (WHO grade 2) gliomas aged 40 years or older without regard to the extent of resection or younger than 40 years old with subtotal resection or biopsy. Eligible participants will be randomly assigned to receive radiation therapy (RT) alone or RT plus temozolomide chemotherapy in a 1:1 ratio. The same RT will be given to all eligible participants regardless of whether they are randomly assigned to RT group or chemoradiotherapy (CRT) group. While in the CRT group, patients will receive adjuvant TMZ with or without concurrent radiochemotherapy. The primary outcome of this trial is progression-free survival and it will be analyzed by intention-to-treat (ITT). Secondary outcomes include OS, adverse events and cognitive function (CF).

Discussion
The objective of our research is to assess the effect of radiotherapy coupled with temozolomide in high-risk LGGs after surgery, compared with RT alone. Different histological types and molecular subtypes will be examined and subgroup analysis will be conducted based on them. Our data can provide evidence for postoperative adjuvant therapy in Chinese high-risk LGGs.

Trial Registration
Chinese Clinical Trial Registry, ChiCTR1800015199. Registered on 13th March, 2018. http://www.chictr.org.cn
Evaluation of the surveillance system for invasive meningococcal disease (IMD) in the Netherlands, 2004 – 2016
Authors: Brandwagt Diederik A.H. , Ende Arie van der , Ruijs Helma (W.) L.M. , de Melker Hester E. , Knol Mirjam J. 
Published: March 2019

Abstract
Background
Enhanced surveillance for confirmed cases of invasive meningococcal disease (IMD) was introduced in the Netherlands in 2003, in which reference laboratory data (NRLBM) are linked with notification data (OSIRIS). The quality of surveillance information is important for public health decision making. Our objective was to describe the system and evaluate it for data completeness and timeliness. Methods Cases reported in the surveillance system from 2004 to 2016 were included. For the notification data, we used information on serogroup, vaccination status, mortality, and country of infection as indicators for record completeness. Notification times to regional and national level were calculated using the reported dates available in the notification database.  Results A total of 2,123 cases were reported in the years 2004-2016, of which 1.968 (93%) were reported by the reference laboratory and 1.995 (94%) in the notification system. Of all cases, 1.840 cases (87%) were reported in both systems and could be linked. The serogroup was known in 86% of the notified cases, and was significantly higher (94%) in the years 2013-2016. Information on vaccination status, mortality and country of infection was available in 88%, 99% and 97% of notified cases, respectively. Regional notification of cases occurred within one working day for 86% of cases and 98% were notified nationally within three days.  Conclusions A well performing IMD surveillance system was demonstrated and serogroup completeness has improved over the years. Underlining the need for reporting to both the clinical and laboratory surveillance system remains important to further improve the overall performance in supporting public health response and vaccination policy.
New Insight into Genotypic and Phenotypic Relatedness of Staphylococcus aureus Strains from Human Infections or Animal Reservoirs
Authors: LISOWSKA-ŁYSIAK KLAUDIA , KOSECKA-STROJEK MAJA , BIAŁECKA JOANNA , KASPROWICZ ANDRZEJ , GARBACZ KATARZYNA , PIECHOWICZ LIDIA , KMET VLADIMIR , SAVINI VINCENZO , MIĘDZOBRODZKI JACEK 
Published: March 2019

Abstract

Staphylococcus aureus is a common human and livestock opportunistic pathogen, and there is evidence of animal to human transmission. This paper aimed to recognize properties of the isolates from collections of human and livestock S. aureus strains and to estimate compatibility of results based on phenotypic tests, microarrays and the spa typing methods. The second goal was to study differences between human and animal isolates in terms of specificity of their hosts and the strain transmission among various hosts. Most strains showed multi-susceptible profiles and produced enzymes on a high level, and they were phenotypically and genetically similar. However, in contrast to the Polish bovine mastitis strains, the Slovakian strains were multi-resistant. In this research, the strains showed significant differences in terms of their phenotypic manifestations and the presence of hemolysins genes; however, other enzyme-encoding genes correlated to a higher extent with the microarrays results. Interestingly, there was a lack of enterotoxin genes in human Poultry-like protein A+ strains in comparison to other human strains. Our study showed that differences between virulence profiles of the human and animal strains correlated with their origin rather than their hosts, and any trait allowed clearly distinguishing between them based on the microarray results.
The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment
Authors: WITA AGNIESZKA , BIAŁAS WOJCIECH , WILK RADOSŁAW , SZYCHOWSKA KATARZYNA , CZACZYK KATARZYNA 
Published: March 2019

Abstract
Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase. Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed. Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world. In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture. The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method. Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification. Optimization of biosynthesis of the cellulose-degrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21–42°C (depending on the strain and carbon source). The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains. These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).