bluuebunny/crossref_metadata_2025_split huggingface dataset:
Life history and biocontrol potential of the first female-producing parthenogenetic species of Diglyphus (Hymenoptera: Eulophidae) against agromyzid leafminers
Authors: Ye Fu-Yu , Zhu Chao-Dong , Yefremova Zoya , Liu Wan-Xue , Guo Jian-Yang , Wan Fang-Hao 
Published: February 2018

AbstractDiglyphusspecies are dominant biocontrol agents for suppressing outbreaks of agromyzid leafminers in fields. In July 2015, we collected a new thelytokous species ofDiglyphusin Qinghai, China. The wasp is here named asD.waniLiu, Zhu & Yefremova sp. nov., based on morphological and molecular analyses. The life history and biocontrol potential of the wasp were studied in the laboratory and by providingLiriomyza sativaelarvae. The intrinsic rate of increase, finite rate of increase, and mean generation time were 0.2373 d−1, 1.2678 d−1, and 15.9 d, respectively. The wasps showed three types of host-killing behaviour, namely parasitism, host feeding and host stinging, resulting in 47.6, 94.4 and 3.4 host larvae killed per wasp over a lifetime, respectively. The finite total host-killing rate was 1.0071 and the wasp showed strong synovigeny. The wasp may be a crucial biocontrol agent against agromyzid leafminers.
Debye formulas for a relaxing system with memory
Authors: Klik Ivo , McHugh James , Chantrell Roy W. , Chang Ching-Ray 
Published: February 2018

AbstractRate (master) equations are ubiquitous in statistical physics, yet, to the best of our knowledge, a rate equation with memory has previously never been considered. We write down an integro-differential rate equation for the evolution of a thermally relaxing system with memory. For concreteness we adopt as a model a single-domain magnetic particle driven by a small ac field and derive the modified Debye formulas. For any memory time Θ the in-phase component of the resultant ac susceptibility is positive at small probing frequencies ω, but becomes negative at large ω. The system thus exhibits frequency induced diamagnetism. For comparison we also consider particle pairs with dipolar coupling. The memory effect is found to be enhanced by ferromagnetic coupling and suppressed by antiferromagnetic coupling. Numerical calculations support the prediction of a negative susceptibility which arises from a phase shift induced by the memory effect. It is proposed that the onset of frequency induced diamagnetism represents a viable experimental signature of correlated noise.
New Zealand glowworm (Arachnocampa luminosa) bioluminescence is produced by a firefly-like luciferase but an entirely new luciferin
Authors: Watkins Oliver C. , Sharpe Miriam L. , Perry Nigel B. , Krause Kurt L. 
Published: February 2018

AbstractThe New Zealand glowworm, Arachnocampa luminosa, is well-known for displays of blue-green bioluminescence, but details of its bioluminescent chemistry have been elusive. The glowworm is evolutionarily distant from other bioluminescent creatures studied in detail, including the firefly. We have isolated and characterised the molecular components of the glowworm luciferase-luciferin system using chromatography, mass spectrometry and 1H NMR spectroscopy. The purified luciferase enzyme is in the same protein family as firefly luciferase (31% sequence identity). However, the luciferin substrate of this enzyme is produced from xanthurenic acid and tyrosine, and is entirely different to that of the firefly and known luciferins of other glowing creatures. A candidate luciferin structure is proposed, which needs to be confirmed by chemical synthesis and bioluminescence assays. These findings show that luciferases can evolve independently from the same family of enzymes to produce light using structurally different luciferins.
Controlling striatal function via anterior frontal cortex stimulation
Authors: van Holstein Mieke , Froböse Monja I. , O’Shea Jacinta , Aarts Esther , Cools Roshan 
Published: February 2018

AbstractMotivational, cognitive and action goals are processed by distinct, topographically organized, corticostriatal circuits. We aimed to test whether processing in the striatum is under causal control by cortical regions in the human brain by investigating the effects of offline transcranial magnetic stimulation (TMS) over distinct frontal regions associated with motivational, cognitive and action goal processing. Using a three-session counterbalanced within-subject crossover design, continuous theta burst stimulation was applied over the anterior prefrontal cortex (aPFC), dorsolateral prefrontal cortex, or premotor cortex, immediately after which participants (N = 27) performed a paradigm assessing reward anticipation (motivation), task (cognitive) switching, and response (action) switching. Using task-related functional magnetic resonance imaging (fMRI), we assessed the effects of stimulation on processing in distinct regions of the striatum. To account for non-specific effects, each session consisted of a baseline (no-TMS) and a stimulation (post-TMS) fMRI run. Stimulation of the aPFC tended to decrease reward-related processing in the caudate nucleus, while stimulation of the other sites was unsuccessful. A follow-up analysis revealed that aPFC stimulation also decreased processing in the putamen as a function of the interaction between all factors (reward, cognition and action), suggesting stimulation modulated the transfer of motivational information to cortico-striatal circuitry associated with action control.
Therapeutic effects of diclofenac, pregabalin, and duloxetine on disuse-induced chronic musculoskeletal pain in rats
Authors: Ohmichi Yusuke , Ohmichi Mika , Murai Nobuhito , Yasui Masaya , Takeshita Nobuaki , Oshibuchi Hidehiro , Naito Munekazu , Nakano Takashi , Sato Jun 
Published: February 2018

AbstractThe aim of this study was to clarify the mechanism of disuse-induced muscle hyperalgesia through the evaluation of the pharmacological behaviour of muscle hyperalgesia profiles in chronic post-cast pain (CPCP) rats with acute and chronic-phase mirror-image muscle hyperalgesia treated with diclofenac (NSAID), pregabalin (an inhibitor of Ca2+ channel α2δ), and duloxetine (SNRI). After 2 weeks of cast immobilization, the peak cross-sectional area and muscle wet weight of the ipsilateral soleus and gastrocnemius muscles decreased more significantly in CPCP rats than in untreated rats. Histological findings revealed disuse-induced muscle atrophy in CPCP rats. The blood biochemical parameters of CPCP rats in acute and chronic phases did not differ significantly from those of untreated rats. The diclofenac and pregabalin-treated groups exhibited no improvement in acute or chronic muscle hyperalgesia. In contrast, the duloxetine-treated group exhibited an improvement in acute muscle hyperalgesia, but showed no apparent effect on chronic muscle hyperalgesia on ipsilateral or contralateral sides. However, the chronic muscle hyperalgesia was reversed by intrathecal administration of DAMGO (a μ-opioid receptor agonist). The results suggest that chronic muscle hyperalgesia in CPCP rats did not result from an inflammatory mechanism, and there is only a low probability that it’s caused by a neuropathic mechanism.
Multiple Kernel Learning Model for Relating Structural and Functional Connectivity in the Brain
Authors: Surampudi Sriniwas Govinda , Naik Shruti , Surampudi Raju Bapi , Jirsa Viktor K. , Sharma Avinash , Roy Dipanjan 
Published: February 2018

AbstractA challenging problem in cognitive neuroscience is to relate the structural connectivity (SC) to the functional connectivity (FC) to better understand how large-scale network dynamics underlying human cognition emerges from the relatively fixed SC architecture. Recent modeling attempts point to the possibility of a single diffusion kernel giving a good estimate of the FC. We highlight the shortcomings of the single-diffusion-kernel model (SDK) and propose a multi-scale diffusion scheme. Our multi-scale model is formulated as a reaction-diffusion system giving rise to spatio-temporal patterns on a fixed topology. We hypothesize the presence of inter-regional co-activations (latent parameters) that combine diffusion kernels at multiple scales to characterize how FC could arise from SC. We formulated a multiple kernel learning (MKL) scheme to estimate the latent parameters from training data. Our model is analytically tractable and complex enough to capture the details of the underlying biological phenomena. The parameters learned by the MKL model lead to highly accurate predictions of subject-specific FCs from test datasets at a rate of 71%, surpassing the performance of the existing linear and non-linear models. We provide an example of how these latent parameters could be used to characterize age-specific reorganization in the brain structure and function.
Filling the Gaps in the Kirromycin Biosynthesis: Deciphering the Role of Genes Involved in Ethylmalonyl-CoA Supply and Tailoring Reactions
Authors: Robertsen Helene L. , Musiol-Kroll Ewa M. , Ding Ling , Laiple Kristina J. , Hofeditz Torben , Wohlleben Wolfgang , Lee Sang Yup , Grond Stephanie , Weber Tilmann 
Published: February 2018

AbstractKirromycin is the main product of the soil-dwelling Streptomyces collinus Tü 365. The elucidation of the biosynthetic pathway revealed that the antibiotic is synthesised via a unique combination of trans-/cis-AT type I polyketide synthases and non-ribosomal peptide synthetases (PKS I/NRPS). This was the first example of an assembly line integrating the three biosynthetic principles in one pathway. However, information about other enzymes involved in kirromycin biosynthesis remained scarce. In this study, genes encoding tailoring enzymes KirM, KirHVI, KirOI, and KirOII, and the putative crotonyl-CoA reductase/carboxylase KirN were deleted, complemented, and the emerged products analysed by HPLC-HRMS and MS/MS. Derivatives were identified in mutants ΔkirM, ΔkirHVI, ΔkirOI, and ΔkirOII. The products of ΔkirOI, ΔkirOII, and kirHVI were subjected to 2D-NMR for structure elucidation. Our results enabled functional assignment of those enzymes, demonstrating their involvement in kirromycin tailoring. In the ΔkirN mutant, the production of kirromycin was significantly decreased. The obtained data enabled us to clarify the putative roles of the studied enzymes, ultimately allowing us to fill many of the missing gaps in the biosynthesis of the complex antibiotic. Furthermore, this collection of mutants can serve as a toolbox for generation of new kirromycins.
Evolving Bacterial Fitness with an Expanded Genetic Code
Authors: Tack Drew S. , Cole Austin C. , Shroff Raghav , Morrow Barrett R. , Ellington Andrew D. 
Published: February 2018

AbstractSince the fixation of the genetic code, evolution has largely been confined to 20 proteinogenic amino acids. The development of orthogonal translation systems that allow for the codon-specific incorporation of noncanonical amino acids may provide a means to expand the code, but these translation systems cannot be simply superimposed on cells that have spent billions of years optimizing their genomes with the canonical code. We have therefore carried out directed evolution experiments with an orthogonal translation system that inserts 3-nitro-L-tyrosine across from amber codons, creating a 21 amino acid genetic code in which the amber stop codon ambiguously encodes either 3-nitro-L-tyrosine or stop. The 21 amino acid code is enforced through the inclusion of an addicted, essential gene, a beta-lactamase dependent upon 3-nitro-L-tyrosine incorporation. After 2000 generations of directed evolution, the fitness deficit of the original strain was largely repaired through mutations that limited the toxicity of the noncanonical. While the evolved lineages had not resolved the ambiguous coding of the amber codon, the improvements in fitness allowed new amber codons to populate protein coding sequences.
Cooling aerosols and changes in albedo counteract warming from CO2 and black carbon from forest bioenergy in Norway
Authors: Arvesen Anders , Cherubini Francesco , del Alamo Serrano Gonzalo , Astrup Rasmus , Becidan Michael , Belbo Helmer , Goile Franziska , Grytli Tuva , Guest Geoffrey , Lausselet Carine , Rørstad Per Kristian , Rydså Line , Seljeskog Morten , Skreiberg Øyvind , Vezhapparambu Sajith , Strømman Anders Hammer 
Published: February 2018

AbstractClimate impacts of forest bioenergy result from a multitude of warming and cooling effects and vary by location and technology. While past bioenergy studies have analysed a limited number of climate-altering pollutants and activities, no studies have jointly addressed supply chain greenhouse gas emissions, biogenic CO2 fluxes, aerosols and albedo changes at high spatial and process detail. Here, we present a national-level climate impact analysis of stationary bioenergy systems in Norway based on wood-burning stoves and wood biomass-based district heating. We find that cooling aerosols and albedo offset 60–70% of total warming, leaving a net warming of 340 or 69 kg CO2e MWh−1 for stoves or district heating, respectively. Large variations are observed over locations for albedo, and over technology alternatives for aerosols. By demonstrating both notable magnitudes and complexities of different climate warming and cooling effects of forest bioenergy in Norway, our study emphasizes the need to consider multiple forcing agents in climate impact analysis of forest bioenergy.
Assessment of soil heavy metals for eco-environment and human health in a rapidly urbanization area of the upper Yangtze Basin
Authors: Jia Zhongmin , Li Siyue , Wang Li 
Published: February 2018

AbstractSoil pollution with heavy metals (HMs) has been attracting more and more interests, however, assessment of eco-environmental and human risks particularly in a rapidly urbanization area (the upper Yangtze) remains limited. Multiple modern indices were firstly performed for complete risk assessment of eco-environment and human health based on a high-spatial-resolution sampling. Averages of HMs were far below grade II threshold level of the Chinese Environmental Quality standards for soils, whereas Cd, As and Hg considerably exceeded the local background values. EF suggested overall moderate enrichments of Cd and Se, resulting in soils uncontaminated to moderately contaminated with them. Potential ecological risk index showed significant differences among Counties that were characterized by moderate risk. However, several sites were moderately to heavily contaminated with As, Cd and Hg by Igeo, resulting in that these sites were categorized as “considerable risk”, or “high risk”. Moreover, children were more susceptible to the potential health risk irrespective of the carcinogenic or non – carcinogenic risk. There were no significant carcinogenic and non – carcinogenic risks for adults, children however showed significant non – carcinogenic effect. Our first assessment provided important information for policy making to reduce the potential effects of soil contamination on human and eco-environment.
Common Deregulation of Seven Biological Processes by MicroRNAs in Gastrointestinal Cancers
Authors: Zhang Lin , Zhang Yuchen , Wong Sunny H. , Law Priscilla T. Y. , Zhao Shan , Yu Jun , Chan Matthew T. V. , Wu William K. K. 
Published: February 2018

AbstractMicroRNAs are frequently dysregulated in human neoplasms, including gastrointestinal cancers. Nevertheless, the global influence of microRNA dysregulation on cellular signaling is still unknown. Here we sought to elucidate cellular signaling dysregulation by microRNAs in gastrointestinal cancers at the systems biology level followed by experimental validation. Signature dysregulated microRNAs in gastric, colorectal and liver cancers were defined based on our previous studies. Targets of signature dysregulated miRNAs were predicted using multiple computer algorithms followed by gene enrichment analysis to identify biological processes perturbed by dysregulated microRNAs. Effects of microRNAs on endocytosis were measured by epidermal growth factor (EGF) internalization assay. Our analysis revealed that, aside from well-established cancer-related signaling pathways, several novel pathways, including axon guidance, neurotrophin/nerve growth factor signaling, and endocytosis, were found to be involved in the pathogenesis of gastrointestinal cancers. The regulation of EGF receptor (EGFR) endocytosis by two predicted miRNAs, namely miR-17 and miR-145, was confirmed experimentally. Functionally, miR-145, which blocked EGFR endocytosis, prolonged EGFR membrane signaling and altered responsiveness of colon cancer cells to EGFR-targeting drugs. In conclusion, our analysis depicts a comprehensive picture of cellular signaling dysregulation, including endocytosis, by microRNAs in gastrointestinal cancers.
In silico guided reconstruction and analysis of ICAM-1-binding var genes from Plasmodium falciparum
Authors: Carrington Eilidh , Otto Thomas D. , Szestak Tadge , Lennartz Frank , Higgins Matt K. , Newbold Chris I. , Craig Alister G. 
Published: February 2018

AbstractThe Plasmodium falciparum variant surface antigen PfEMP1 expressed on the surface of infected erythrocytes is thought to play a major role in the pathology of severe malaria. As the sequence pool of the var genes encoding PfEMP1 expands there are opportunities, despite the high degree of sequence diversity demonstrated by this gene family, to reconstruct full-length var genes from small sequence tags generated from patient isolates. To test whether this is possible we have used a set of recently laboratory adapted ICAM-1-binding parasite isolates to generate sequence tags and, from these, to identify the full-length PfEMP1 being expressed by them. In a subset of the strains available we were able to produce validated, full-length var gene sequences and use these to conduct biophysical analyses of the ICAM-1 binding regions.
Cocaine-induced locomotor sensitization associates with slow oscillatory firing of neurons in the ventral tegmental area
Authors: Liu Chang-Liang , Wang Ya-Kun , Jin Guo-Zhang , Shi Wei-Xing , Gao Ming 
Published: February 2018

AbstractThe initiation of psychostimulant sensitization depends on the mesocorticolimbic dopamine (DA) system. Although many cellular adaptations has been reported to be associated with this addictive behavior, the overall influence of these adaptations on the network regulation of DA neurons has not been established. Here, we profile a network-driven slow oscillation (SO) in the firing activity of ventral tegmental area (VTA) putative DA and non-DA neurons and their correlation with locomotor sensitization induced by repeated administration of cocaine. One day after the last cocaine injection, the power of SO (Pso) significantly increased both in DA and non-DA neurons. Interestingly, the Pso in DA neurons was positively correlated, while Pso in non-DA neurons was negatively correlated with the level of locomotor sensitization. On the other hand, the firing rates of DA and non-DA neurons were both elevated, but none exhibited any correlation with the level of sensitization. Fourteen days after the last injection, the Pso of DA neurons dissipated but still positively correlated with the level of sensitization. In contrast, the Pso in non-DA neurons lost correlation with locomotor sensitization. These results suggest that cocaine-induced locomotor sensitization is associated with long-term network adaptation in DA system and that DA and non-DA neurons may corporately facilitate/hamper the initiation of locomotor sensitization.
Understanding the functional role of genistein in the bone differentiation in mouse osteoblastic cell line MC3T3-E1 by RNA-seq analysis
Authors: Kim Myungsuk , Lim Jisun , Lee Jung-Hee , Lee Kyung-Mi , Kim Suji , Park Kye Won , Nho Chu Won , Cho Yoon Shin 
Published: February 2018

AbstractGenistein, a phyto-estrogen, can potentially replace endogenous estrogens in postmenopausal women, but the underlying molecular mechanisms remain incompletely understood. To obtain insight into the effect of genistein on bone differentiation, RNA sequencing (RNA-seq) analysis was used to detect differentially expressed genes (DEGs) in genistein-treated vs. untreated MC3T3-E1 mouse osteoblastic cells. Osteoblastic cell differentiation was monitored by measuring osteoblast differentiation factors (ALP production, bone mineralization, and expression of osteoblast differentiation markers). From RNA-seq analysis, a total of 132 DEGs (including 52 up-regulated and 80 down-regulated genes) were identified in genistein-treated cells (FDR q-value < 0.05 and fold change > 1.5). KEGG pathway and Gene Ontology (GO) enrichment analyses were performed to estimate the biological functions of DEGs and demonstrated that these DEGs were highly enriched in functions related to chemotactic cytokines. The functional relevance of DEGs to genistein-induced osteoblastic cell differentiation was further evaluated by siRNA-mediated knockdown in MC3T3-E1 cells. These siRNA knockdown experiments (of the DEGs validated by real-time qPCR) demonstrated that two up-regulated genes (Ereg and Efcab2) enhance osteoblastic cell differentiation, while three down-regulated genes (Hrc, Gli, and Ifitm5) suppress the differentiation. These results imply their major functional roles in bone differentiation regulated by genistein.
Decontamination of Petroleum-Contaminated Soils Using The Electrochemical Technique: Remediation Degree and Energy Consumption
Authors: Streche Constantin , Cocârţă Diana Mariana , Istrate Irina-Aura , Badea Adrian Alexandru 
Published: February 2018

AbstractCurrently, there are different remediation technologies for contaminated soils, but the selection of the best technology must be not only the treatment efficiency but also the energy consumption (costs) during its application. This paper is focused on assessing energy consumption related to the electrochemical treatment of polluted soil with petroleum hydrocarbons. In the framework of a research project, two types of experiments were conducted using soil that was artificially contaminated with diesel fuel at the same level of contamination. The experimental conditions considered for each experiment were: different amounts of contaminated soils (6 kg and 18 kg, respectively), the same current intensity level (0.25A and 0.5A), three different contamination degrees (1%, 2.5% and 5%) and the same time for application of the electrochemical treatment. The remediation degree concerning the removal of petroleum hydrocarbons from soil increased over time by approximately 20% over 7 days. With regard to energy consumption, the results revealed that with an increase in the quantity of treated soil of approximately three times, the specific energy consumption decreased from 2.94 kWh/kg treated soil to 1.64 kWh/kg treated soil.