bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adverse events associated with continuous interscalene block administered using the catheter-over-needle method: a retrospective analysis
Authors: Tanizima Meishu , Takechi Kenichi , Nakanishi Kazuo , Yorozuya Toshihiro 
Published: October 2019

Abstract
Background: Continuous interscalene block is widely used for pain management in shoulder surgery. However, continuous interscalene block performed using the catheter-through-needle method is reportedly associated with adverse events such as pericatheter leakage of the local anesthetic, phrenic nerve paralysis, and hoarseness. Because we expected that the catheter-over-needle method would reduce these adverse events, we examined cases in which continuous interscalene block was performed using the catheter-over-needle method to determine what adverse events occurred and when.
Methods: We retrospectively reviewed the anesthesia and medical records of adult patients who underwent catheter insertion to receive a continuous interscalene block performed using the catheter-over-needle method at our hospital from July 2015 to July 2017.
Results: During the surveillance period, 122 adult patients underwent catheter insertion to receive a continuous interscalene block administered using the catheter-over-needle method. No case of pericatheter local anesthetic leakage was observed. Adverse events, such as dyspnea, hoarseness, insufficient anesthetic effect, dizziness, cough reflex during drinking, or ptosis, were observed in 42 patients (34.4%; 95% confidence interval 26–42.7). Most of the adverse events occurred on postoperative day 2. The median time between surgery and the onset of adverse events was 28.5 h.
Conclusions: The catheter-over-needle method may prevent the pericatheter leakage of the local anesthetic. However, adverse events occurred in more than one-third of the patients. During continuous interscalene block, patients must be carefully observed for adverse events, especially on postoperative day 2.
Trial registration: This study was registered with the UMIN Clinical Trials Registry (UMIN000037673).
Regulatory Roles of Differentially Expressed MicroRNAs in Metabolic Processes in Negative Lens-Induced Myopia Guinea Pigs
Authors: Guo Dadong , Ding Meihua , Song Xiaoli , Sun Yuanyuan , Li Guoping , Li Zonghong , Wei Huixia , Wu Jianfeng , Jiang Wenjun , Bi Hongsheng 
Published: October 2019

Abstract
Myopia is one of the most common vision defects worldwide. microRNAs can regulate the target gene expressions, influencing the development of diseases. In order to investigate the alterations of microRNAs profiling in negative lens-induced myopia (NLIM) guinea pigs and to explore the regulatory role of microRNAs in the occurrence and the development of myopia, we first established the NLIM guinea pig model after induction for 2 weeks. Further, we isolated sclera to purify total messenger RNA (mRNA) in both NLIM and NLIM fellow sclera. Using next generation sequencing technique and bioinformatics analysis, we identified the differentially expressed microRNAs in NLIM guinea pigs, performed the bioinformatics annotation for the differentially expressed microRNAs, and validated the expressions of differentially expressed microRNAs. We successfully established a negative NLIM model in guinea pigs, identified 27 differentially expressed microRNAs in NLIM guinea pig sclera, including 10 up-regulated and 17 down-regulated microRNAs. The KEGG annotation showed the main signaling pathways were closely associated with PPAR signaling, pyruvate and propanoate metabolisms, and TGF-beta signaling pathways. In summary, we found the development of myopia is mainly involved in the disorder of metabolic processes in NLIM guinea pigs. The PPAR signaling, pyruvate and propanoate metabolism pathways may play roles in the development of myopia.
Validation of a New Risk Score System for Non-variceal Upper Gastrointestinal Bleeding
Authors: Kim Min Seong , Moon Hee Seok , Kwon In Sun , Park Jae Ho , Kim Ju Seok , Kang Sun Hyung , Sung Jae Kyu , Lee Eaum Seok , Kim Seok Hyun , Lee Byung Seok , Jeong Hyun Yong 
Published: October 2019

Abstract
Background Recently, a new international bleeding score was developed to predict 30-day hospital mortality in patients with upper gastrointestinal bleeding (UGIB). However, the efficacy of this newly developed scoring system has not been extensively investigated. We aimed to validate a new scoring system for predicting 30-day mortality in patients with non-variceal UGIB and determine whether a higher score is associated with re-bleeding, length of hospital stay and endoscopic failure.Methods A retrospective study was performed on 905 cases with acute non-varieal UGIB who were examined in our hospital between January 2013 and December 2017. Baseline characteristics, endoscopic findings, re-bleeding, admission, and mortality were reviewed. The 30-day mortality rate of the new international bleeding risk score was calculated using the receiver operating characteristic curves and compared to the pre-endoscopy Rockall score, AIMS65, Glasgow Blatchford score, and Progetto Nazionale Emorragia Digestiva score. To verify the variable for the 30-day mortality of the new scoring system, we performed multivariate logistic regression using our data and further analyzed the score items.Results Compared with other scoring systems, the new international bleeding scoring system showed higher receiver operating characteristic (ROC) curve values in predicting mortality. (area under ROC curve 0.832; [95% confidence interval (CI)]) Multivariate analysis was performed using our data, the 30-day mortality rate was related to multiple comorbidities, BUN, creatinine, albumin, syncope at first visit, and endoscopic failure at first admission within 24 hours. In addition, in the new high score group, relatively long hospital stays, re-bleeding, and endoscopic failure were observed.Conclusion The new international bleeding score could predict 30-day mortality better than the other scoring systems. High-risk patients can be screened using this new scoring system to predict 30-day mortality. The use of this scoring system seems to improve the outcomes of non-variceal UGIB patients through proper management and intervention.
Risk factors of pulmonary complications after minimally invasive surgery for elderly patients with vertebral compression fractures
Authors: Zhang Hua-Xing , Shen Yong , Chen Jia , Zhang Long , Lin Wei 
Published: October 2019

Abstract
Purpose: To determine the risk factors for pulmonary complications after minimally invasive surgery in elderly patients with vertebral compression fractures (VCFs). Methods: We retrospectively analyzed 233 elderly patients (age ≥ 65 years) with VCFs who underwent percutaneous vertebroplasty (PVP) or percutaneous kyphoplasty (PKP) surgery at Hebei General Hospital from January 2011 to December 2016 . Risk factors and the effects of the model were determined by univariate logistic regression analyses and the receiver operating characteristic (ROC) curve, respectively. A risk assessment scale was established based on the risk factors and physiological and surgical scores for mortality and morbidity. The risk assessment scale prospectively evaluated risk factors of pulmonary complications after minimally invasive surgery for elderly patients with VCFs from January to June 2017. Results: A total of 27 patients diagnosed with pulmonary complications (11.59%) among 233 detected patients. There were statistically significant differences in age, body mass index (BMI), smoking, cardiovascular diseases and old fractures between patients with and without pulmonary complications (P<0.05). Logistic regression analysis showed that smoking, cardiovascular diseases and old fractures were risk factors of pulmonary complications after PVP or PKP for elderly patients with VCFs (P<0.05) and area under the curve was 0.738 (95% confidence intervals (CI): 0.648-0.828). We assessed 53 elderly patients with VCFs, 5 of whom occurred pulmonary complications after PVP or PKP. Areas under the curve of preoperative and total risk assessment values were all 0.925. Conclusions: Significant risk factors of pulmonary complications were BMI, cardiovascular diseases and old fractures for patients aged 65 years or elderly with VCFs after minimally invasive surgery. The risk assessment scale established by us gaining high accuracy.
Information needs and information seeking behaviour of dementia patients and their caregivers: a scoping review
Authors: Soong Aijia , Au Shu Ting , Kyaw Bhone Myint , Theng Yin Leng , Car Lorainne Tudor 
Published: October 2019

Abstract
Background Patients with dementia often require full-time caregivers especially in the later stages of their condition. Patients and caregivers’ access to reliable information on dementia is essential as it may have an important impact on patient care and quality of life. This study aims to provide an overview of the information needs and information seeking behaviour of dementia patients and their caregivers.Methods We conducted a scoping review of the literature and searched four electronic databases for eligible studies published up to August 2018. Two reviewers independently screened studies and extracted data. Information needs were classified according to emerging themes in the literature, and information seeking behaviour was categorized using Wilson’s model of information behaviour.Results Twenty studies with a total of 4,140 participants, were included in this review. Reported information needs focused on: (i) disease; (ii) patient care provision; (iii) healthcare services; and (iv) caregiver self-care. The most commonly reported information need was on healthcare service-related information. Characteristics found to influence information needs were the severity of dementia as well as patient and caregiver status. Dementia patients and caregivers mainly displayed active searching information seeking behaviour and preferred using electronic sources to obtain health information.Conclusion Current dementia information sources available in English are extensive in the information they offer, but more emphasis needs to be placed on healthcare service-related information. All studies originated from high income countries and focused on information needs of caregiver only. The only variable found to significantly influence information needs was severity of dementia condition. The information needs identified in this review can be used to inform development and design of future dementia resources for patients and their caregivers.
Comparison of Outcomes Postoperation between Straight Leg Raising Test Negative and Positive Patients Who Underwent Percutaneous Transforaminal Endoscopic Discectomy for Lumbar Disc Herniation
Authors: Wei Feilong , Gao Haoran , Yuan Yifang , Qian Shu , Guo Quanyou , Guo Shikong , Xue Weigao , Zhou Chengpei , Qian Jixian 
Published: October 2019

Abstract
Background: Percutaneous Transforaminal Endoscopic Discectomy is used increasingly in patients with Lumbar Disc Herniation. There is little knowledge on the related factors including SLR test influencing the operation. Therefore, we designed this prospective study to explore the relevant factors influencing postoperative effect of PTED surgery.Methods: Consecutive patients with LDH who came to our hospital from August 2015 to September 2016 and received PTED surgery. 4 kinds of scales including VAS (lumbar/leg), ODI and JOA were measured and reassessed at 1 day, 3 months, 6 months, 12months and 36 months after the PTED to assess their surgical outcomes. Results: All the patients had successful surgery. ODI and VAS (lumbar/leg) decreased in all patients and groups. And there was a statistically significant difference in each postoperative follow-up compared with that before surgery in every visit. In addition, the increase of JOA in postoperation was statistically significant compared with that before surgery. And, there is statistically significant difference between the three subpopulations (patients with SLR Positive (0°-30°), SLR Positive (31°-60°) and SLR Negative (61°-) in the changes of the scores of VAS(leg), ODI and JOA. However, there is no statistically significant difference between the three subpopulations (patients with SLR Positive (0°-30°), SLR Positive (31°-60°) and SLR Negative (61°--RRB- in the changes of the score of VAS(lumbar). Conclusions: PTED showed great effect on treating patients with lumbar disc herniation. And the main scale score such as VAS(leg). ODI and JOA showed that there is a statistically significant difference between the three subpopulations treated by PTED. Patients with SLR negative may get greater benefit from PTED.
Prevalence and contributing factors of Birth asphyxia among the neonates delivered at Negest Eleni Mohammed memorial teaching hospital, Southern Ethiopia: A cross-sectional study
Authors: Abdo Ritbano Ahmed , Halil Hassen Mosa , Kebede Biruk Assefa , Anshebo Abebe Alemu , Gejo Negeso Gebeyehu 
Published: October 2019

Abstract
Background: Birth asphyxia is a major contributor to neonatal mortality worldwide. In Ethiopia, birth asphyxia remains a severe condition that leads to significant mortality and morbidity. This study aimed to assess the prevalence and contributing factors of birth asphyxia among the neonates delivered at the Negest Eleni Mohammed Memorial Teaching Hospital, Southern Ethiopia.
Methods: A hospital based cross-sectional study design was employed from June 1- 30, 2019. Two hundred seventy nine study participants were selected using the systematic sampling method. Data were collected using a pretested structured interviewer administered questionnaire, check list and chart review. Data were entered into Epi-data (version 3.1) and analyzed using statistical package for social sciences (version 24). Descriptive and binary logistic regression analysis were made and P value of < 0.05 was considered significant.
Result: The overall prevalence of birth asphyxia among newborns was found to be 15.1%. Factors that were significantly associated with birth asphyxia included mothers aged ≥ 35 [AOR=6.4, 95% CI (2.0, 20.5)], primigravida [AOR=5.1, 95% CI (2.0, 13.3)], prolonged second stage of labor [AOR=4.6, 95%CI (1.6, 13.3)], preterm birth [AOR=4.7, 95% CI (1.5, 14.1)], meconium stained amniotic fluid[AOR=7.5, 95% CI (2.5, 21.4)]and tight nuchal[AOR=3.1, 95% CI (1.2, 9.3)].
Conclusion: Birth asphyxia is still prevalent in the study setting. The results of this study show the need for better maternal care, creating awareness about contributing factors of birth asphyxia to the maternity health professionals, careful monitoring of labor, and identifying and taking proper measures that could help in reducing the occurrence of birth asphyxia.
Proteome Profile Changes During Poly-hydroxybutyrate Intracellular Mobilization in Gram Positive Bacillus cereus tsu1
Authors: LI HUI , O’Hair Joshua , Thapa Santosh , Bhatti Sarabjit , Zhou Suping , Yang Yong , Fish Tara , Thannhauser Theodore W. 
Published: October 2019

Abstract
Background Bacillus cereus is a bacterium species can grow efficiently on a wide range of carbon sources and accumulate biopolymer poly-hydroxybutyrate (PHB) up to 80% cell dry weight. PHB is an aliphatic polymer produced and stored intracellularly as a reservoir of carbon and energy, its mobilization is a key biological process for the spore forming Bacillus spp . Our lab previously isolated and identified a bacterial strain Bacillus cereus tsu1. The bacterium was cultured on rapeseed cake substrate (RCS) and the maxima of PHB accumulation reached within 12 h, and depleted after 48 h; fore-spore and spore structure were observed after 24 h culture.Results Quantitative proteomic analysis of ‘ tsu1’ identified 3,215 proteins in total, out of which 2,952 got quantified. 244 were identified as significantly changed proteins (SCPs) in the 12-24-hour pair of samples, and 325 in the12-48-hour pair of samples. Gene Ontology classification revealed that enriched biological processes only found in the 12-24h pair SCPs include purine nucleotide metabolism, protein folding, metal ion homeostasis, response to stress, carboxylic acid catabolism, cellular amino acid catabolism; and those only found in the 12-48h pair SCPs were carbohydrate metabolism, protein metabolism, oxidative phosphorylation, formation of translation ternary structure. Based on statistical analysis, key enzymes poly(R)-hydroxyalkanoic acid synthase (PhaC, KGT44864) is significantly higher in 12h-culture; SigF, SpoEII related with sporulation were significantly higher in the 24h-samples; enzymes for nitrate respiration and fermentation were significantly induced in 48h-culture.Conclusions Proteome profile changes during PHB intracellular mobilization in B. cereus tsu1 were characterized in this study. The key enzyme PhaC for PHB synthesis is significantly higher in 12h-culture which supports the highest PHB accumulation at this time point; protein abundance of SpoIIE and σF were significantly increased to induce sporulation in 24h-culture; enzymes for nitrate respiration and fermentation were significantly induced in 48h-culture which implied the depletion of oxygen at this stage and carbon flow towards fermentative growth. The results from this study provide insights into proteome profile changes during PHB accumulation and reuse, which can be applied to achieve a higher PHB yield and to improve bacterial growth performance and stress resistance.
Assessment of Care Provision for Hypertension at Emergency Department of an Urban Hospital in Mozambique
Authors: Bay Neusa Vanessa Amad , JUGA Edna , MACUACUA Carlos , JOAO Jose , ANIBAL Maria , STEWART Simon , MOCUMBI Ana Olga 
Published: October 2019

Abstract
Background: Management of hypertension in Mozambique is poor, and rates of control are amongst the lowest in the world. Health system related factors contribute at least partially to this situation, particularly in settings where there is scarcity of resources to address the double burden of infectious and non-communicable diseases. This study aimed to assess the management of hypertension in an emergency department (ED). Methods: During a pragmatic and prospective 30-day snapshot study (with 24 hour surveillance) and random profiling of one-in-five presentations to the ED of Hospital Geral De Mavalane, Maputo, we assessed patient’s flow, infrastructure and resources through direct observation, and reports from pharmacy and laboratory stocks were used to assess availability of diagnostics and medicines needed for hypertension management. Results: Hypertensive patients had several stops during their journey inside the health facility, and followed a non-standardized flow. No clinical protocols or algorithms for risk stratification of hypertension were available. Stock-outs of basic diagnostic tools for risk stratification and medicines were registered. The availability of medicines was 28% on average. Conclusions: Critical gaps in health facility readiness to address arterial hypertension seen in ED were uncovered, including lack of clinical protocols, insufficient availability of diagnostics and essential medicines, as well as low affordability of the families to guaranty continuum of care. Innovative financing mechanisms are needed to support the health system to address hypertension.
Epidemic Ribotypes Of Clostridium (Now Clostridioides) Difficile Are Likely To Be More Virulent Than Non-epidemic Ribotypes In Animal Models
Authors: Vitucci John C. , Pulse Mark , Tabor-Simecka Leslie , Simecka Jerry W. 
Published: October 2019

Abstract
Background. Clostridium difficile infections have become more frequently diagnosed and associated with greater disease severity, which has resulted in an increase burden on the healthcare system. These increases are attributed to the increased prevalence of hypervirulent strains encompassing select ribotypes. These epidemic ribotypes were characterized as hypervirulent due to higher in vitro spore and toxin production, as well as increased incidence, severity and mortality within patients. However, it is unclear whether epidemic ribotypes are truly more virulent than non-epidemic ribotypes in vivo. Furthermore, there is conflicting evidence about the ability of a strain’s in vitro phenotype to be predictive of their in vivo virulence. The goals of the current studies were to determine if epidemic ribotypes are more virulent than other ribotypes in animal models, and whether the in vitro virulence phenotype of an isolate or ribotype predict in vivo virulence. Results. To determine if epidemic strains were truly more virulent than other non-epidemic strains, the in vivo virulence of thirteen C. difficile isolates (7 non-epidemic and 6 epidemic ribotype isolates) were determined in murine (C57BL/6 mice) and hamster (golden Syrian hamster) models of C. difficile infections. The isolates of epidemic ribotype of C. difficile were found to be more virulent in both the murine and hamster models than non-epidemic isolates. In particular, the group of epidemic ribotypes of C. difficile had lower LD50 values in hamsters. The increased severity of disease was associated with higher levels of Toxin A and Toxin B production found in fecal samples, but not numbers of organisms recovered. The isolates were further characterized for their in vitro virulence phenotypes, e.g. toxin production, growth rates, spore formation and adherence of spores to intestinal epithelial cell lines. Although there were higher levels of toxins produced and greater adherence for the group of epidemic ribotypes, the in vitro profiles of individual isolates were not always predictive of their in vivo virulence. Conclusions. Overall, the group of epidemic ribotypes of C. difficile were more virulent in vivo despite individual isolates having similar phenotypes to the non-epidemic isolates in vitro.
A Comparison of Two DNA Metagenomic Bioinformatic Pipelines while evaluating the Microbial Diversity in feces of Tanzanian small holder dairy cattle
Authors: Kibegwa Felix , Francesca Stomeo , Rawlynce Bett C. , Charles Gachuiri K. , Fidalis Mujibi D. 
Published: October 2019

Abstract
Background: Analysis of shotgun metagenomic data generated from next generation sequencing platforms can be done through a variety of bioinformatic pipelines. These pipelines employ different sets of sophisticated bioinformatics algorithms which may affect the results of this analysis. Furthermore, no conventional assessment technique for estimating the precision of each pipeline exists and few studies have been carried out to compare the characteristics, benefits and disadvantages of each pipeline. In this study we compared two commonly used pipelines for shotgun metagenomic analysis: MetaGenome Rapid Annotation using Subsystem Technology (MG-RAST) and Kraken, in terms of taxonomic classification, diversity analysis and usability using their primarily default parametersResults: Overall, the two pipelines detected similar abundance distributions in the three most abundant taxa Proteobacteria, Firmicutes and Bacteroidetes. Within bacterial domain, 497 genera were identified by both pipelines, while an additional 694 and 98 genera were solely identified by Kraken and MG-RAST respectively. 933 species were detected by the two algorithms. Kraken solely detected 3550 species, while MG-RAST identified 557 species uniquely. For archaea, Kraken generated 105 and 236 genera and species respectively while MG-RAST detected 60 genera and 88 species. 54 genera and 72 species were commonly detected by the two methods. Kraken had a quicker analysis time (~4 hours) while MG-RAST took approximately 2 days per sample.Conclusions: This study revealed that Kraken and MG-RAST generate comparable results and that a reliable high-level overview of sample is generated irrespective of the pipeline selected. The observed variations at the genus level show that a main restriction is using different databases for classification of the metagenomic data. Specifically, the pipelines could have been limited because some rumen microbes lack reference genomes. The results of this research indicate that a more inclusive and representative classification of microbiomes may be achieved through creation of combined pipelines.