bluuebunny/crossref_metadata_2025_split huggingface dataset:
A community engaged primary healthcare strategy to address rural school student inequities: a descriptive paper
Authors: Jones Debra , Ballard Jacqueline , Dyson Robert , Macbeth Peter , Lyle David , Sunny Palatty , Thomas Anu , Sharma Indira 
Published: March 2019

AbstractAimThis descriptive paper aims to describe the design and implementation of a community engaged primary healthcare strategy in rural Australia, the Primary Healthcare Registered Nurse: Schools-Based strategy. This strategy seeks to address the health, education and social inequities confronting children and adolescents through community engaged service provision and nursing practice.BackgroundThere have been increasing calls for primary healthcare approaches to address rural health inequities, including contextualised healthcare, enhanced healthcare access, community engagement in needs and solutions identification and local-level collaborations. However, rural healthcare can be poorly aligned to community contexts and needs and be firmly entrenched in health systems, marginalising community participation.MethodsThis strategy has been designed to enhance nursing service and practice responsiveness to the rural context, primary healthcare principles, and community experiences and expectations of healthcare. The strategy is underpinned by a cross-sector collaboration between a local health district, school education and a university department of rural health. A research framework is being developed to explore strategy impacts for service recipients, cross-sector systems, and the establishment and maintenance of a primary healthcare nursing workforce.FindingsAlthough in the early stages of implementation, key learnings have been acquired and strategic, relationship, resource and workforce gains achieved.
An Investigation of Hydrate Formation in Unsaturated Sediments Using X‐Ray Computed Tomography
Authors: Lei Liang , Liu Zhichao , Seol Yongkoo , Boswell Ray , Dai Sheng 
Published: March 2019

AbstractPhysical properties of hydrate‐bearing sediments are often correlated with hydrate saturation with little or no information on hydrate distribution uniformity in the specimens. This study focuses on water redistribution and sediment skeleton shift depending on various hydrate formation conditions in unsaturated systems, as well as on the resulting hydrate distribution patterns. Using X‐ray computed tomography, we investigate the factors such as fines content and the pressure‐temperature path on mass migration during carbon dioxide hydrate formation. The experiments show water migration, preferential hydrate formation toward the core periphery, localized patchy hydrate distribution, and sediment particle movement toward the core center. Sediment particle movement can be impeded in densely packed specimens. The overall mass migration due to hydrate formation can be significantly suppressed by adding 5% by mass of kaolinite. Hydrate formation initiated by pressurization and then cooling causes less mass migration than the cases where hydrate is formed using cooling followed by pressurization or pressurizing frozen cores followed by heating methods. Freezing can induce water migration and particle pushing in a similar manner as hydrate formation. Image analyses show that the pressure‐temperature path and the rates of heat transfer during hydrate nucleation and growth govern the uniformity of hydrate distribution in sediments.
Deciphering the chronology of copy number alterations in Multiple Myeloma
Authors: Aktas Samur Anil , Minvielle Stephane , Shammas Masood , Fulciniti Mariateresa , Magrangeas Florence , Richardson Paul G. , Moreau Philippe , Attal Michel , Anderson Kenneth C. , Parmigiani Giovanni , Avet-Loiseau Hervé , Munshi Nikhil C. , Samur Mehmet Kemal 
Published: March 2019

AbstractMultiple myeloma (MM) and its precursor condition MGUS are characterized by chromosomal aberrations. Here, we comprehensively characterize the order of occurrence of these complex genomic events underlying MM development using 500 MGUS, and MM samples. We identify hyperdiploid MM (HMM) and non-HMM as genomically distinct entities with different evolution of the copy number alterations. In HMM, gains of 9,15 or 19 are the first and clonal events observed as clonal even at MGUS stage. These events are thus early and may underlie initial transformation of normal plasma cells to MGUS cells. However, CNAs may not be adequate for progression to MM except in 15% of the patients in whom the complex subclonal deletion events are observed in MM but not MGUS. In NHMM, besides the driver translocations, clonal deletion of 13 and 1q gain are early events also observed in MGUS. We combined this information to propose a timeline for copy number alteration.
Modeling magnetic-field-induced domain wall propagation in modulated-diameter cylindrical nanowires
Authors: Fernandez-Roldan J. A. , De Riz A. , Trapp B. , Thirion C. , Vazquez M. , Toussaint J.-C. , Fruchart O. , Gusakova D. 
Published: March 2019

AbstractDomain wall propagation in modulated-diameter cylindrical nanowires is a key phenomenon to be studied with a view to designing three-dimensional magnetic memory devices. This paper presents a theoretical study of transverse domain wall behavior under the influence of a magnetic field within a cylindrical nanowire with diameter modulations. In particular, domain wall pinning close to the diameter modulation was quantified, both numerically, using finite element micromagnetic simulations, and analytically. Qualitative analytical model for gently sloping modulations resulted in a simple scaling law which may be useful to guide nanowire design when analyzing experiments. It shows that the domain wall depinning field value is proportional to the modulation slope.
Tunable Schottky barrier in graphene/graphene-like germanium carbide van der Waals heterostructure
Authors: Wang Sake , Chou Jyh-Pin , Ren Chongdan , Tian Hongyu , Yu Jin , Sun Changlong , Xu Yujing , Sun Minglei 
Published: March 2019

AbstractThe structural and electronic properties of van der Waals (vdW) heterostructrue constructed by graphene and graphene-like germanium carbide were investigated by computations based on density functional theory with vdW correction. The results showed that the Dirac cone in graphene can be quite well-preserved in the vdW heterostructure. The graphene/graphene-like germanium carbide interface forms a p-type Schottky contact. The p-type Schottky barrier height decreases as the interlayer distance decreases and finally the contact transforms into a p-type Ohmic contact, suggesting that the Schottky barrier can be effectively tuned by changing the interlayer distance in the vdW heterostructure. In addition, it is also possible to modulate the Schottky barrier in the graphene/graphene-like germanium carbide vdW heterostructure by applying a perpendicular electric field. In particular, the positive electric field induces a p-type Ohmic contact, while the negative electric field results in the transition from a p-type to an n-type Schottky contact. Our results demonstrate that controlling the interlayer distance and applying a perpendicular electric field are two promising methods for tuning the electronic properties of the graphene/graphene-like germanium carbide vdW heterostructure, and they can yield dynamic switching among p-type Ohmic contact, p-type Schottky contact, and n-type Schottky contact in a single graphene-based nanoelectronics device.
Hydrogen-terminated diamond field-effect transistor with AlOx dielectric layer formed by autoxidation
Authors: Wang Yan-Feng , Wang Wei , Chang Xiaohui , Zhang Xiaofan , Fu Jiao , Liu Zhangcheng , Zhao Dan , Shao Guoqing , Fan Shuwei , Bu Renan , Zhang Jingwen , Wang Hong-Xing 
Published: March 2019

AbstractFabrication of hydrogen-terminated diamond (H-diamond) field-effect transistor (FET) with AlOx dielectric layer has been successfully carried out. The AlOx layer was formed by auto-oxidizing 6 nm Al film in the air at room temperature, and a FET without AlOx dielectric layer has also been fabricated for comparison. For both FETs, 100 nm Al layers were deposited as the gate electrodes, respectively. The leakage current density in FET with AlOx dielectric layer was four magnitude orders lower than that without AlOx dielectric layer at VGS = −5 V, indicating that AlOx dielectric layer could effectively reduce leakage current and prevent reverse ID in ID − VDS caused by defects on diamond surface. Distinct pinch-off characteristic with p-type channel was observed in ID − VDS measurement. The threshold voltage was −0.4 V at VDS = −15 V.
Local-Field Corrections as a Regularization Method for the Spin-Boson Model
Authors: Crosse J. A. 
Published: March 2019

AbstractThe decoherence rate of a ‘central spin’ in a bosonic bath of magnetic fluctuations is computed using the spin-boson model. The magnetic fluctuations are treated in a fully quantum mechanical way by using the macroscopic quantum electrodynamics formalism and are expressed in terms of the classical electromagnetic Green’s function of the system. The resulting frequency integral formally diverges but it can be regularized by applying real-cavity, local-field corrections to the location of the ‘central spin’. This results in a cut-off function in terms of the magnetic permeability of the background material that leads to convergence at both high and low frequencies. This cut-off function appears naturally from the formalism and thus removes the need to rely on ad-hoc arguments to justify the form of the cut-off function. Furthermore, the magnetic permeability and the nature of interactions in quantum electrodynamics illuminate the connection between the two main models of ‘central spin’ decoherence, the spin-boson model and the spin-bath model, demonstrating how the two very different models are able to correctly model the same underlying physics.
Unveiling a Scaling and Universal Behavior for the Magnetocaloric Effect in Cubic Crystal Structures: A Monte Carlo Simulation
Authors: Alzate-Cardona J. D. , Salcedo-Gallo J. S. , Rodríguez-Patiño D. F. , Acosta-Medina C. D. , Restrepo-Parra E. 
Published: March 2019

AbstractThe magnetocaloric effect and the universal character for the magnetic entropy change regarding the cubic crystal structures (SC, BCC, FCC) were investigated, in a qualitative way, using Monte Carlo simulations. A classical Heisenberg Hamiltonian with nearest neighbors, and next nearest neighbors interactions was implemented. In order to compute the critical temperature of the system depending on the coordination number, it was calculated the dependence of the magnetization and magnetic susceptibility as a function of temperature. Magnetic field dependence on the magnetization for isothermal processes was performed considering a magnetocrystalline anisotropy term. In this way, the magnetic entropy change (ΔSm) was computed. Results show that the rescaled ΔSm as well as the exponent (n) characterizing the field dependence of the magnetic entropy change curves, collapse onto a single curve for the studied crystal structures. By this reason, it can be assured that ΔSm exhibits a universal behavior regarding the strength and contribution of the magnetic exchange energy to the total magnetic energy.
A plasmonic refractive index sensor with an ultrabroad dynamic sensing range
Authors: Cheng Yu-Chieh , Chang Ya-Ju , Chuang Yu-Ching , Huang Bo-Zhi , Chen Chii-Chang 
Published: March 2019

AbstractRefractive index sensors based on surface plasmon resonance (SPR) promise to deliver high sensitivities. However, these sensitivities depend on the derivative of the monitored SPR parameters near resonance, so this dependency leads to a relatively narrow detection range for refractive index changes. Herein, we introduce an idea to improve the detection range refractive index through a high-contrast-index curved waveguide surrounded with an outer gold ring. The proposed detection technique, based on the output power measurement of the curved waveguide, offers a linear response over an ultrabroad range of the refractive index for a surrounding medium from n = 1 to 2.36. Meanwhile, an theoretically ultrahigh refractive index resolution (RIU) of 4.53 × 10−10 could be accessible for such a broad testing range, available for both gas and aqueous chemical sample refractive indices Furthermore, the power detection approach enables an integrated photodetector for a lab-on-chip sensor platform, revealing a high potential for a multifunctional, compact, and highly sensitive sensor-on-chip device.
Effects of sublethal doses of clothianidin and/or V. destructor on honey bee (Apis mellifera) self-grooming behavior and associated gene expression
Authors: Morfin Nuria , Goodwin Paul H. , Hunt Greg. J. , Guzman-Novoa Ernesto 
Published: March 2019

AbstractLittle is known about the combined effects of stressors on social immunity of honey bees (Apis mellifera) and related gene expression. The interaction between sublethal doses of a neurotoxin, clothianidin, and the ectoparasite, Varroa destructor, was examined by measuring differentially expressed genes (DEGs) in brains, deformed wing virus (DWV) and the proportion and intensity of self-grooming. Evidence for an interaction was observed between the stressors in a reduction in the proportion of intense groomers. Only the lowest dose of clothianidin alone reduced the proportion of self-groomers and increased DWV levels. V. destructor shared a higher proportion of DEGs with the combined stressors compared to clothianidin, indicating that the effects of V. destructor were more pervasive than those of clothianidin when they were combined. The number of up-regulated DEGs were reduced with the combined stressors compared to clothianidin alone, suggesting an interference with the impacts of clothianidin. Clothianidin and V. destructor affected DEGs from different biological pathways but shared impacts on pathways related to neurodegenerative disorders, like Alzheimer’s, which could be related to neurological dysfunction and may explain their negative impacts on grooming. This study shows that the combination of clothianidin and V. destructor resulted in a complex and non-additive interaction.
Activin A regulates the epidermal growth factor receptor promoter by activating the PI3K/SP1 pathway in oral squamous cell carcinoma cells
Authors: Tsai Chi-Neu , Tsai Chia-Lung , Yi Jui-Shan , Kao Huang-Kai , Huang Yenlin , Wang Chun-I , Lee Yun-Shien , Chang Kai-Ping 
Published: March 2019

AbstractEpidermal growth factor receptor (EGFR) and activin A are both overexpressed in oral cavity squamous cell carcinoma (OSCC). We evaluated their clinical correlation and activin A-mediated EGFR regulation in this study. Overexpression of both transcripts/proteins indicated a poorer prognosis in OSCC patients. Knockdown of endogenous INHBA repressed the expression of EGFR and inhibited activin A-mediated canonical Smads, noncanonical phosphorylation of AKT (ser473) (p-AKT ser473) and SP1. Inhibition of PI3K signaling via its inhibitor attenuated p-AKT ser473 and in turn reduced SP1 and EGFR expression in the presence of recombinant activin A (rActivin A) in OSCC cells, as revealed via a luciferase assay and western blotting. However, canonical Smad signaling repressed the EGFR promoter, as revealed by a luciferase assay. The transcription factor SP1, its coactivator CBP/p300, and Smad proteins were recruited to the EGFR proximal promoter following rActivin A treatment, as revealed by chromatin immunoprecipitation (ChIP). Smad2/3/4 dramatically outcompeted SP1 binding to the EGFR proximal promoter following mithramycin A treatment. Activin A activates the PI3K and Smad pathways to compete for binding to overlapping SP1 consensus sequences on the EGFR proximal promoter. Nevertheless, canonical p-Smad2 was largely repressed in OSCC tumor tissues, suggesting that the activin A-mediated noncanonical pathway is essential for the carcinogenesis of OSCC.
Effect of Mechanical Stretch on the DNCB-induced Proinflammatory Cytokine Secretion in Human Keratinocytes
Authors: Oh Seunghee , Chung Hyewon , Chang Sooho , Lee Su-Hyon , Seok Seung Hyeok , Lee Hyungsuk 
Published: March 2019

AbstractSkin is exposed to various physico-chemical cues. Keratinocytes, a major component of the skin epidermis, directly interact with the surrounding extracellular matrix, and thus, biochemical and biophysical stimulations from the matrix regulate the function of keratinocytes. Although it was reported that inflammatory responses of skin were altered by an applied mechanical force, understanding how the keratinocytes sense the mechanical stimuli and regulate a cytokine secretion remains unclear. Here, we designed a device that is able to apply chemo-mechanical cues to keratinocytes and assess their proinflammatory cytokine IL-6 production. We showed that when chemical stimuli were applied with mechanical stimuli simultaneously, the IL-6 production markedly increased compared to that observed with a single stimulus. Quantitative structural analysis of cellular components revealed that the applied mechanical stretch transformed the cell morphology into an elongated shape, increased the cell size, and dictated the distribution of focal adhesion complex. Our results suggest that the mechanical cue-mediated modulation of focal adhesion proteins and actin cytoskeleton translates into intracellular signaling associated with the IL-6 production particularly in skin sensitization. Our study can be applied to understand proinflammatory responses of skin under altered biophysical environments of the skin.
Mass production of poly(ethylene glycol) monooleate-modified core-shell structured upconversion nanoparticles for bio-imaging and photodynamic therapy
Authors: Zhang Xingyuan , Guo Zhao , Zhang Xiao , Gong Linji , Dong Xinghua , Fu Yanyan , Wang Qing , Gu Zhanjun 
Published: March 2019

AbstractDeveloping robust and high-efficient synthesis approaches has significant importance for the expanded applications of upconversion nanoparticles (UCNPs). Here, we report a high-throughput synthesis strategy to fabricate water-dispersible core-shell structured UCNPs. Firstly, we successfully obtain more than 10 grams core UCNPs with high quality from one-pot reaction using liquid rare-earth precursors. Afterwards, different core-shell structured UCNPs are fabricated by successive layer-by-layer strategy to get enhanced fluorescence property. Finally, the hydrophobic UCNPs are modified with poly(ethylene glycol) monooleate (PEG-OA) though a novel physical grinding method. On the basis of mass-production, we use the as-prepared PEG-UCNPs to construct an 808-nm stimuli photodynamic therapy agent, and apply them in cancer therapy and bio-imaging.
Context-dependent activity of A domains in the tyrocidine synthetase
Authors: Degen Anna , Mayerthaler Florian , Mootz Henning D. , Di Ventura Barbara 
Published: March 2019

AbstractNon-ribosomal peptide synthetases (NRPSs) are large, modular enzymes that produce bioactive peptides of tremendous structural and chemical diversity, due to the incorporation, alongside the canonical 20 amino acids, of non-proteinogenic amino acids, fatty acids, sugars and heterocyclic rings. For linear NRPSs, the size and composition of the peptide product is dictated by the number, order and specificity of the individual modules, each made of several domains. Given the size and complexity of NRPSs, most in vitro studies have focused on individual domains, di-domains or single modules extracted from the full-length proteins. However, intermodular interactions could play a critical role and regulate the activity of the domains and modules in unpredictable ways. Here we investigate in vitro substrate activation by three A domains of the tyrocidine synthetase TycC enzyme, systematically comparing their activity when alone (with the respective PCP domain), in pairs (di-modular constructs) or all together (tri-modular construct). Furthermore, we study the impact of mutations in the A or PCP domains in these various constructs. Our results suggest that substrate adenylation and effects of mutations largely depend on the context in which the domains/modules are. Therefore, generalizing properties observed for domains or modules in isolation should be done with caution.
Parasite detection in the ornamental fish trade using environmental DNA
Authors: Trujillo-González A. , Edmunds R. C. , Becker J. A. , Hutson K. S. 
Published: March 2019

AbstractEffective border control relies on stringent biosecurity protocols to detect and prevent introductions of exotic pests and diseases. Detection of pathogens and parasites in the live ornamental fish trade using environmental DNA (eDNA) techniques has the potential to improve current biosecurity practices. We examined water samples from 11 target consignments (cyprinids susceptible to Dactylogyrus spp. infections) and seven non-target fish consignments (non-cyprinids, not susceptible to Dactylogyrus spp. infections) imported from Southeast Asia to Australia for the presence of eDNA from five Dactylogyrus species (Monogenea: Dactylogyridae). A four-step predictive framework was used to predict putative positive and putative negative detections from quantitative PCR assays. Both target and non-target consignments were positive for Dactylogyrus spp. eDNA as confirmed by Sanger sequencing. Positive detections for Dactylogyrus spp. eDNA in non-target fish consignments demonstrates the possibility of source water contamination, limiting the applicability of eDNA screening methods at border control. This study suggests that screening for parasite eDNA within ornamental fish consignments should be tested during pre-export quarantine periods to avoid false positive detections at border control. Lastly, the proposed predictive framework has a broad utility for minimizing false positive and false negative eDNA detections of aquatic organisms.
Theoretical analysis on thermodynamic stability of chignolin
Authors: Sumi Tomonari , Koga Kenichiro 
Published: March 2019

AbstractUnderstanding the dominant factor in thermodynamic stability of proteins remains an open challenge. Kauzmann’s hydrophobic interaction hypothesis, which considers hydrophobic interactions between nonpolar groups as the dominant factor, has been widely accepted for about sixty years and attracted many scientists. The hypothesis, however, has not been verified or disproved because it is difficult, both theoretically and experimentally, to quantify the solvent effects on the free energy change in protein folding. Here, we developed a computational method for extracting the dominant factor behind thermodynamic stability of proteins and applied it to a small, designed protein, chignolin. The resulting free energy profile quantitatively agreed with the molecular dynamics simulations. Decomposition of the free energy profile indicated that intramolecular interactions predominantly stabilized collapsed conformations, whereas solvent-induced interactions, including hydrophobic ones, destabilized them. These results obtained for chignolin were consistent with the site-directed mutagenesis and calorimetry experiments for globular proteins with hydrophobic interior cores.