bluuebunny/crossref_metadata_2025_split huggingface dataset:
When does it pay to be green? The strategic benefits of adoption speed
Authors: Su Hung‐Chung , Fu Wayne , Linderman Kevin 
Published: October 2024

AbstractDoes the speed of adopting environmental practices impact financial benefits? The strategy literature discusses the contingencies under which firms can gain an early‐mover advantage or a late‐mover advantage. This research examines the effect of adoption speed on two types of environmental practices: environmental innovation practices (EIP) and environmental management practices (EMP). The results show that early adoption of EIP increases competitive advantage when firms face intense competition. In comparison, we show that early adoption of EMP increases competitive advantage when firms face extremely low competition or have moderate to high levels of slack resources. The study contributes to the literature by revealing the nuances, contingencies, and boundary conditions of when it pays to be green. Prior research shows mixed results when studying firms' decisions to implement environmental practices, which implies that it may not pay to be green. This study shows that firms can get an early mover advantage from environmental practices, but it depends on the type of environmental practices, the firm's internal slack resources, and the firm's external competitive environment.
Synergy Between Woody Peat and Bentonite Alters Stability of Soil Organic Carbon in Coarse Soil by Enhancing Capacity for Soil Aggregation and Hydro‐Physical Properties
Authors: Wang Xi , Fan Jun , Wang Huan , Du Mengge 
Published: October 2024

ABSTRACTCoarse soil has a poor structure and is susceptible to wind and water erosion, thereby making it difficult to maintain the soil organic carbon (SOC) content. Woody peat (WP) is an organic material that can increase the SOC content of the soil, while clay materials can rapidly enhance the capacity for soil aggregate formation. In order to explore the synergistic effects of WP and clay materials (bentonite and red clay) on the aggregate structure and hydro‐physical properties of coarse soil, as well as the mechanism associated with SOC mineralization (ΔSOC), we conducted an incubation study using lou soil (L0) and loessial soil (H0) with three treatments: addition of WP alone (LW, HW), mixture of WP and bentonite (LWB, HWB), and mixture of WP and red clay (LWR, HWR). The three treatments enhanced the proportion of macroaggregate (> 2 mm) and aggregate stability of the two soils, and optimized the water retention and ventilation performance. The highest aggregate stability of LWB and HWB can be attributed to the positive synergistic effect of WP and bentonite, and bentonite was more effective than red clay due to its crystal structure. The results also showed that the ΔSOC values were significantly lower under LWB and HWB than those under WP addition alone and adding the mixture of WP and red clay (p < 0.05). Moreover, partial least squares path modeling analysis showed that the hydro‐physical properties of the two improved soils inhibited SOC mineralization (p > 0.05), whereas particulate organic carbon (POC) content significantly accelerated SOC mineralization (p < 0.01). The synergistic effect of clay materials increased mineral‐associated organic carbon (MAOC), which was beneficial to maintain the long‐term effectiveness of WP. Overall, our results demonstrated that the synergistic use of WP and bentonite enhanced the aggregate stability and hydro‐physical properties of coarse soil and improved SOC storage capacity. These results provide scientific and theoretical guidance to facilitate the rapid improvement of coarse soil through engineering measures in arid and semi‐arid areas with water and fertilizer limitations.
Effect of different calcium sulfate aspect ratios and chitosan addition methods on the interface properties of calcium sulfate/chitosan composite bone cement
Authors: Jiang Liuyun , Tang Chunyan , Tang Shuo , Wang Yuqing , Ouyang Qi , Hu Xiang 
Published: October 2024

AbstractIt is a great challenge to improve the properties of pure calcium sulfate bone cement. Here, two calcium sulfate hemihydrate powders with different aspect ratios were prepared, and three chitosan addition methods were adopted to investigate the effect on the surface and interface properties of calcium sulfate/chitosan composite bone cement. The results showed that it lacked chemical bond between chitosan and calcium sulfate, and the short rod calcium sulfate displayed better interface adhesion with chitosan owing to its smaller size, so it possessed shorter setting time, better compressive strength and slower degradation than the long rod calcium sulfate, while the biocompatibility had no remarkable difference. Moreover, chitosan solution as liquid phase was a better solidification mode than citric acid, and calcium sulfate/chitosan composite as solid phase was the worst mode because of poor interface compatibility. Conclusively, it was a simple, low‐cost, and effective preparation process to choose short rod calcium sulfate powder as solid phase and 1 wt% chitosan solution as liquid phase, which could achieve calcium sulfate/chitosan composite bone cement with the best properties, including setting time, compressive strength, degradation rate, and biocompatibility, displaying a promising application in bone defect repair.
A prediction model for differential resilience to the effects of combat‐related stressors in US army soldiers
Authors: Kessler Ronald C. , Bossarte Robert M. , Hwang Irving , Luedtke Alex , Naifeh James A. , Nock Matthew K. , Petukhova Maria , Sadikova Ekaterina , Sampson Nancy A. , Sverdrup Erik , Zubizarreta Jose R. , Wager Stefan , Wagner James , Stein Murray B. , Ursano Robert J. 
Published: October 2024

AbstractObjectivesTo develop a composite score for differential resilience to effects of combat‐related stressors (CRS) on persistent DSM‐IV post‐traumatic stress disorder (PTSD) among US Army combat arms soldiers using survey data collected before deployment.MethodsA sample of n = 2542 US Army combat arms soldiers completed a survey shortly before deployment to Afghanistan and then again two to three and 8–9 months after redeployment. Retrospective self‐reports were obtained about CRS. Precision treatment methods were used to determine whether differential resilience to persistent PTSD in the follow‐up surveys could be developed from pre‐deployment survey data in a 60% training sample and validated in a 40% test sample.Results40.8% of respondents experienced high CRS and 5.4% developed persistent PTSD. Significant test sample heterogeneity was found in resilience (t = 2.1, p = 0.032), with average treatment effect (ATE) of high CRS in the 20% least resilient soldiers of 17.1% (SE = 5.5%) compared to ATE = 3.8% (SE = 1.2%) in the remaining 80%. The most important predictors involved recent and lifetime pre‐deployment distress disorders.ConclusionsA reliable pre‐deployment resilience score can be constructed to predict variation in the effects of high CRS on persistent PTSD among combat arms soldiers. Such a score could be used to target preventive interventions to reduce PTSD or other resilience‐related outcomes.
Inflammatory arthropathies: Perspectives from a Portuguese male individual (1574–1834 CE)
Authors: Antunes‐Ferreira Nathalie , Curate Francisco , Prates Carlos , Marques Carina 
Published: October 2024

AbstractArthropathies are common in past populations and can be categorized into two groups: those with predominant bone production (e.g., osteoarthritis) and those with significant bone loss (e.g., erosive arthropathies). The former is frequent in the archaeological record, whereas the latter are uncommon. We present a Post‐Medieval male individual, recovered in the Convent of the Holy Spirit (Loures, Portugal), with multiple articular and entheseal bone changes, particularly extensive periarticular, marginal, and subchondral erosive processes, often exposing trabecular bone. Proliferative lesions and extensive ankylosis are also observed in the synovial joints. These pathological changes affect both the axial and peripheral skeleton in a polyarticular, bilateral, and asymmetric pattern. Given that the appendicular skeleton, particularly the hands and feet, are the most affected areas, the most probable diagnosis is a peripheral spondyloarthropathy such as psoriatic arthritis or reactive arthritis. This case study is the first archaeological instance of psoriatic arthritis or reactive arthritis described in Portugal, highlighting the importance of a differential diagnosis and the need for reflection when pathological changes characteristics overlap, advocating for a broader diagnostic approach.
Guaranteed Cost Control of Impulsive Nonlinear Stochastic Systems With Mixed Time Delays
Authors: Zhao Pengfei , Chen Dongyan , Hu Jun , Zhang Zhiyang , Yang Ning 
Published: October 2024

ABSTRACTThis article mainly addresses the guaranteed cost control (GCC) problem for a class of impulsive nonlinear stochastic systems with mixed time delays, where both distributed time delay as well as discrete time delay are considered. Utilizing the Hamilton–Jacobi inequalities technique, Lyapunov–Krasovskii functional (LKF) and stochastic control theory, a new delay‐dependent sufficient condition is acquired to ensure the asymptotic stability in probability (ASP) of the closed‐loop system (CLS). Moreover, the guaranteed cost controller is designed and the upper bound of cost function is given. At the same time, three corollaries are presented for the special circumstance without impulse, distributed time delays or time‐varying time delay, respectively. Finally, the feasibility of the theoretical results is verified by an example.
Protein Quakes in Redox Metalloenzymes: Clues to Molecular Enzyme Conductivity Triggered by Binding of Small Substrate Molecules
Authors: Bohr Henrik , Shim Irene , Ulstrup Jens , Xiao Xinxin 
Published: October 2024

AbstractMulticentre redox metalloproteins undergo conformational changes on electrochemical surfaces, or on enzyme substrate binding. The two‐centre copper enzymes, laccase (Type I and TypeII/III Cu) and nitrite reductase (CuNIR) (Type I and Type II Cu) are examples. With some exceptions, these enzymes show no non‐turnover voltammetry on Au(111)‐surfaces modified by thiol based self‐assembled molecular monolayers, but dioxygen or nitrite substrate triggers strong electrocatalytic signals. Scanning tunnelling microscopy also shows high conductivity only when dioxygen or nitrite is present. Atomic force microscopy shows constant CuNIR height but pronounced structural expansion in the electrocatalytic range on nitrite binding. We have recently offered a rationale, based on ab initio quantum chemical studies of water/nitrite substitution in a 740‐atom CuNIR fragment. Presently we provide much more detailed structural assignment mapped to single‐residue resolution. NO2−‐binding induces both a 2 Å Cu−Cu distance increase, and pronounced frontier orbital delocalization strongly facilitating ET between the Cu regions. The conformational changes transmit from the catalytic Type II centre to the electron inlet Type I centre, via the His129‐Cys130 ligands, and via Type I–Cys130 or Type I‐His129 ending at Type II Asp92. The ET patterns are reflected in different atomic Mulliken charges in the water and nitrite CuNIR fragment.
Runt‐related transcription factor 1 (RUNX1) is a mediator of acute kidney injury
Authors: Fontecha‐Barriuso Miguel , Villar‐Gomez Natalia , Guerrero‐Mauvecin Juan , Martinez‐Moreno Julio M , Carrasco Susana , Martin‐Sanchez Diego , Rodríguez‐Laguna María , Gómez Manuel J , Sanchez‐Niño María D , Ruiz‐Ortega Marta , Ortiz Alberto , Sanz Ana B 
Published: October 2024

AbstractTreatment for acute kidney injury (AKI) is suboptimal. A better understanding of the pathogenesis of AKI may lead to new therapeutic approaches. Kidney transcriptomics of folic acid‐induced AKI (FA‐AKI) in mice identified Runx1 as the most upregulated RUNX family gene. We then examined the expression of RUNX1 in FA‐AKI, in bacterial lipopolysaccharide (LPS)‐induced cytokine storm‐AKI (CS‐AKI), and in human AKI. In cultured mouse tubule cells, we explored the expression and role of RUNX1 in response to the cytokine TWEAK or LPS. A chemical inhibitor of RUNX1 (Ro5‐3335) was used in animal models of AKI to test its potential as a therapeutic target. RUNX1 overexpression in FA‐AKI was validated at the mRNA and protein levels and localized mainly to tubule cell nuclei. CS‐AKI also upregulated kidney RUNX1. Increased tubule and interstitial RUNX1 expression were also observed in human AKI. In cultured mouse tubule cells, the pro‐inflammatory cytokine TWEAK and LPS increased RUNX1 and IL‐6 expression. Mechanistically, RUNX1 bound to the Il6 gene promoter and RUNX1 targeting with the chemical inhibitor Ro5‐3335, or a specific small interfering RNA (siRNA), prevented the TWEAK‐ and LPS‐induced upregulation of IL6 through a RUNX1/NFκB1 p50 pathway. In vivo, preventive Ro5‐3335 improved kidney function and reduced inflammation in FA‐AKI and CS‐AKI. However, Ro5‐3335 administration after the insult only improved kidney function in CS‐AKI. Kidney transcriptomics identified inflammatory genes and transcription factor mRNAs such as Yap1 and Trp53 as key targets of Ro5‐3335 in CS‐AKI. In conclusion, RUNX1 contributes to AKI by driving the expression of genes involved in inflammation and represents a novel therapeutic target in AKI. © 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.
Understanding the Morphology and Cross‐Link Density in Silicone Rubber‐Multiwalled Carbon Nanotube Composites via Solvent Transport Studies
Authors: Krishnageham Sidharthan Sisanth , Keloth Paduvilan Jibin , Velayudhan Prajitha , Kaliyathan Abitha Vayyaprontavida , Krishnan Remya , Zapotoczny Szczepan , Thomas Sabu 
Published: October 2024

ABSTRACTThe diffusion characteristics of silicone rubber‐based nanocomposites have not been extensively studied in the literature. This study aims to provide a comprehensive analysis of the solvent transport properties of these materials, incorporating detailed dissolution modeling. This study reported a novel approach to elucidate the morphological and diffusion characteristics of silicone rubber‐MWCNT (multiwalled carbon nanotube) composites. FESEM micrograph analysis reveals structural changes with the lower loadings forming continuous networks and higher loadings leading to agglomeration of the fillers. Diffusion studies highlight reduced solvent uptake over time due to compact physical networks, while Kraus plot analysis confirms MWCNTs' reinforcing ability. Dissolution modeling using Korsmeyer–Peppas and Peppas–Sahlin models indicates the type of solvent release behavior, with the latter offering a superior fit. Mode of transport analysis suggests a less Fickian mode influenced by MWCNT loading, while swelling parameters demonstrate hindered solvent transport with increasing MWCNT content. The molecular mass between successive cross‐links and the cross‐link density decreases with rising MWCNT loading, which is theoretically predicted by the affine model. This study also focused on the complex interplay between filler loading, composite structure, and solvent transport behavior in silicone rubber‐MWCNTs composites, offering valuable insights for their potential applications in various fields.
Enhanced cellular death in liver and breast cancer cells by dual BET/BRPF1 inhibitors
Authors: Cazzanelli Giulia , Dalle Vedove Andrea , Sbardellati Nicolò , Valer Luca , Caflisch Amedeo , Lolli Graziano 
Published: October 2024

AbstractThe acetylpyrrole scaffold is an acetylated lysine mimic that has been previously explored to develop bromodomain inhibitors. When tested on the hepatoma cell line Huh7 and the breast cancer cell line MDA‐MB‐231, a few compounds in our acetylpyrrole‐thiazole library induced peculiar morphological changes, progressively causing cell death at increasing concentrations. Their evaluation on a panel of human bromodomains revealed concurrent inhibition of BRPF1 and BET bromodomains. To dissect the observed cellular effects, the acetylpyrrole derivatives were compared to JQ1 and GSK6853, chemical probes for the bromodomains of BET and BRPF1, respectively. The appearance of neurite‐like extrusions, accompanied by βIII‐tubulin overexpression, is caused by BET inhibition, with limited effect on cellular viability. Conversely, interference with BRPF1 induces cellular death but not phenotypic alterations. Combined treatment with JQ1 and GSK6853 showed additivity in reducing cellular viability, comparably to the acetylpyrrole‐thiazole‐based BET/BRPF1 inhibitors. In addition, we determined the crystallographic structures of the BRD4 and BRPF1 bromodomains in complex with the acetylpyrrole‐thiazole compounds. The binding modes in the two bromodomains show similar interactions for the acetylpyrrole and different orientations of the moiety that point to the rim of the acetyl‐lysine pocket.
Protein degradation of antizyme depends on the N‐terminal degrons
Authors: Hsieh Ju‐Yi , Leong Pui‐Ying , Yang Yi‐Fang , Liu Yi‐Liang , Liu Guang‐Yaw , Hung Hui‐Chih 
Published: October 2024

AbstractAntizyme (AZ) is a regulatory protein that plays a crucial role in modulating the activity of ornithine decarboxylase (ODC), which is the initial and rate‐limiting enzyme in the complex pathway of polyamine biosynthesis. AZ facilitates the swift degradation of ODC, thereby modulating the levels of cellular polyamines. This study unveils a new ubiquitin‐independent mechanism for AZ degradation, emphasizing the essential role of N‐terminal degrons. Contrary to traditional ubiquitin‐dependent degradation, our findings reveal that AZ degradation is significantly influenced by its N‐terminal region. By conducting a series of experiments, including in vitro degradation assays, cycloheximide chase experiments, differential scanning calorimetry, and measurement of cellular concentrations of polyamines, we demonstrate that N‐terminal truncation significantly enhances AZ's stability and facilitates the reduction of polyamine levels by accelerating ODC degradation. The removal of the N‐terminal portion of AZ results in a reduced degradation rate and enhanced thermal stability of the protein, leading to a more efficient inhibition of polyamine synthesis. These findings are corroborated by the analysis of AZ isoforms, AZ1, AZ2, and AZ3, which display differential degradation patterns based on the specific N‐terminal segments. This substantiates a degradation mechanism driven by an intrinsically disordered N‐terminal region acting as a degron, independent of lysine ubiquitination. These results underscore the significant regulatory function of the N‐terminal domain in the activity of AZ and the maintenance of polyamine homeostasis.
Acaricide resistance mechanisms and host plant responses in the tomato specialist Aculops lycopersici
Authors: Vervaet Lore , Charamis Jason , Vandenhole Marilou , Vontas John , Van Leeuwen Thomas 
Published: October 2024

AbstractBACKGROUNDThe mite Aculops lycopersici is a major tomato pest with extremely reduced gene families involved in chemoreception and detoxification. How this limited detoxification toolbox affects the evolution of resistance to acaricides in tomato russet mite(s) (TRM) remains enigmatic. Moreover, although a tomato specialist, TRM has been observed on other Solanaceae and Convolvulaceae plant species, raising questions about transcriptional plasticity underlying host exchange.RESULTSWe identified a field strain with strongly decreased susceptibility to both abamectin and spiromesifen. We detected target‐site resistance caused by mutations at conserved positions in two glutamate‐gated chloride channels (GluCl), as well as four overexpressed detoxification genes. We then examined transcriptional responses after host shift from tomato to two Solanaceae (potato and black nightshade) and two Convolvulaceae (sweet potato and hedge bindweed) species, as more challenging host plants. Transcriptional responses varied significantly between host plant families, with key differentially expressed genes (DEGs) related to proteolytic, metabolic and detoxification processes. Last, we also identified DEGs encoding for secreted proteins potentially involved in TRM–host plant interactions.CONCLUSIONSDespite a limited detoxification toolbox, A. lycopersici might quickly evolve target‐site resistance, probably facilitated by strong selection pressure on the genetic variation associated with enormous population size in field settings. Responses to host plant changes include plasticity in genes related to digestion and detoxification, while most responsive genes are of unknown function and remain to be investigated. © 2024 Society of Chemical Industry.
miRNAs targeted transcription factors HaGATAa/b to mediate the post‐mating switch in Helicoverpa armigera female reproductive behavior
Authors: Zhang Songdou , Cai Limei , Wang Yilin , Liu Xiaoxia 
Published: October 2024

AbstractBACKGROUNDThe process of mating induces significant shifts in female reproductive behavior across various species, with the postmating behavioral switch playing a crucial role in insect reproduction. Previous studies have demonstrated the regulatory role of GATA transcription factors in vitellogenin transcription and egg formation in insects, while miRNAs have been implicated in modulating GATA expression and insect reproductive processes. Nevertheless, the precise regulatory mechanism underlying the interaction between miRNAs and GATA transcription factors in the postmating behavioral switch remains largely unexplored.RESULTSIn this study, we identified two key GATA transcription factors, HaGATAa and HaGATAb, as central players in orchestrating the postmating behavior of H. armigera using transcriptomics and RNAi technologies. HaGATAa was found to act upstream of HaGATAb, regulating its expression. Furthermore, we observed a postmating increase in miR‐282 levels in females, targeting HaGATAa to regulate egg‐laying capacity. Conversely, the decreased expression of miR‐2 following mating functioned as a negative feedback regulator, influencing the expression of HaGATAb and thus impacting the postmating behavior of female individuals.CONCLUSIONOur results revealed a signal‐mediated feedback regulatory mechanism that sustains female postmating behavior in H. armigera. These findings not only establish a strong basis for understanding the postmating behavior mechanisms in female moths, but also offer valuable insights for identifying potential targets for pest control strategies. © 2024 Society of Chemical Industry.