bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis
Authors: Fang Yuan , Shen Zhi-Yong , Zhan Yi-Zhi , Feng Xiao-Chuang , Chen Ke-Li , Li Yong-Sheng , Deng Hai-Jun , Pan Su-Ming , Wu De-Hua , Ding Yi 
Published: September 2019

AbstractThe diverse expression pattern of CD36 reflects its multiple cellular functions. However, the roles of CD36 in colorectal cancer (CRC) remain unknown. Here, we discover that CD36 expression is progressively decreased from adenomas to carcinomas. CD36 loss predicts poor survival of CRC patients. In CRC cells, CD36 acts as a tumor suppressor and inhibits aerobic glycolysis in vitro and in vivo. Mechanically, CD36-Glypcian 4 (GPC4) interaction could promote the proteasome-dependent ubiquitination of GPC4, followed by inhibition of β-catenin/c-myc signaling and suppression of downstream glycolytic target genes GLUT1, HK2, PKM2 and LDHA. Moreover, disruption of CD36 in inflammation-induced CRC model as well as ApcMin/+ mice model significantly increased colorectal tumorigenesis. Our results reveal a CD36-GPC4-β-catenin-c-myc signaling axis that regulates glycolysis in CRC development and may provide an intervention strategy for CRC prevention.
Na+-H+ exchanger 1 determines atherosclerotic lesion acidification and promotes atherogenesis
Authors: Liu Cong-Lin , Zhang Xian , Liu Jing , Wang Yunzhe , Sukhova Galina K. , Wojtkiewicz Gregory R. , Liu Tianxiao , Tang Rui , Achilefu Samuel , Nahrendorf Matthias , Libby Peter , Guo Junli , Zhang Jin-Ying , Shi Guo-Ping 
Published: September 2019

AbstractThe pH in atherosclerotic lesions varies between individuals. IgE activates macrophage Na+-H+ exchanger (Nhe1) and induces extracellular acidification and cell apoptosis. Here, we show that the pH-sensitive pHrodo probe localizes the acidic regions in atherosclerotic lesions to macrophages, IgE, and cell apoptosis. In Apoe–/– mice, Nhe1-deficiency or anti-IgE antibody reduces atherosclerosis and blocks lesion acidification. Reduced atherosclerosis in Apoe–/– mice receiving bone marrow from Nhe1- or IgE receptor FcεR1-deficient mice, blunted foam cell formation and signaling in IgE-activated macrophages from Nhe1-deficient mice, immunocomplex formation of Nhe1 and FcεR1 in IgE-activated macrophages, and Nhe1-FcεR1 colocalization in atherosclerotic lesion macrophages support a role of IgE-mediated macrophage Nhe1 activation in atherosclerosis. Intravenous administration of a near-infrared fluorescent pH-sensitive probe LS662, followed by coregistered fluorescent molecular tomography-computed tomography imaging, identifies acidic regions in atherosclerotic lesions in live mice, ushering a non-invasive and radiation-free imaging approach to monitor atherosclerotic lesions in live subjects.
The Functional 3D Organization of Unicellular Genomes
Authors: Ben-Elazar Shay , Chor Benny , Yakhini Zohar 
Published: September 2019

AbstractGenome conformation capture techniques permit a systematic investigation into the functional spatial organization of genomes, including functional aspects like assessing the co-localization of sets of genomic elements. For example, the co-localization of genes targeted by a transcription factor (TF) within a transcription factory. We quantify spatial co-localization using a rigorous statistical model that measures the enrichment of a subset of elements in neighbourhoods inferred from Hi-C data. We also control for co-localization that can be attributed to genomic order. We systematically apply our open-sourced framework, spatial-mHG, to search for spatial co-localization phenomena in multiple unicellular Hi-C datasets with corresponding genomic annotations. Our biological findings shed new light on the functional spatial organization of genomes, including: In C. crescentus, DNA replication genes reside in two genomic clusters that are spatially co-localized. Furthermore, these clusters contain similar gene copies and lay in genomic vicinity to the ori and ter sequences. In S. cerevisae, Ty5 retrotransposon family element spatially co-localize at a spatially adjacent subset of telomeres. In N. crassa, both Proteasome lid subcomplex genes and protein refolding genes jointly spatially co-localize at a shared location. An implementation of our algorithms is available online.
Global invasion genetics of two parasitic copepods infecting marine bivalves
Authors: Feis Marieke E. , Goedknegt M. Anouk , Arzul Isabelle , Chenuil Anne , Boon Onno den , Gottschalck Leo , Kondo Yusuke , Ohtsuka Susumu , Shama Lisa N. S. , Thieltges David W. , Wegner K. Mathias , Luttikhuizen Pieternella C. 
Published: September 2019

AbstractInvasive species, and especially invasive parasites, represent excellent models to study ecological and evolutionary mechanisms in the wild. To understand these processes, it is crucial to obtain more knowledge on the native range, invasion routes and invasion history of invasive parasites. We investigated the consecutive invasions of two parasitic copepods (Mytilicola intestinalis and Mytilicolaorientalis) by combining an extensive literature survey covering the reported putative native regions and the present-day invaded regions with a global phylogeography of both species. The population genetic analyses based on partial COI sequences revealed significant population differentiation for M. orientalis within the native region in Japan, while introduced populations in North America and Europe could not be distinguished from the native ones. Thus, M. orientalis’ invasion history resembles the genetic structure and recent spread of its principal host, the Pacific oyster, Crassostrea gigas, while M. intestinalis lacks population genetic structure and has an overall low genetic diversity. Therefore, the native origin of M. intestinalis remains unclear. With this study, we demonstrate that even highly related and biologically similar invasive species can differ in their invasion genetics. From this, we conclude that extrapolating invasion genetics dynamics from related invasive taxa may not always be possible.
Reduced synchronized brain activity in schizophrenia during viewing of comedy movies
Authors: Tu Pei-Chi , Su Tung-Ping , Lin Wei-Chen , Chang Wan-Chen , Bai Ya-Mei , Li Cheng-Ta , Lin Fa-Hsuan 
Published: September 2019

AbstractPrevious evaluation of brain function in schizophrenia has focused on standard experimental tasks, with cerebral response to natural stimuli less clear. This study employed inter-subject correlation (ISC) analysis to investigate the neural basis of humor processing during free viewing of comedy movies in patients with schizophrenia. We recruited 29 patients diagnosed with schizophrenia and 29 healthy, age- and sex-matched controls. Each participant underwent fMRI scanning during two viewings of three comedy movie clips. The ISC map from each participant pair within each population group and each movie viewing was separately derived. The significance of ISC within a group and between two groups were assessed by bootstrapping. The ISC map from each patient pair were also correlated with the product of Positive and Negative Syndrome Scale (PANSS) rating between the same participant pair in schizophrenia patients. Schizophrenia patients showed significant ISC in bilateral lateraloccipital, bilateral superior frontal, left supramarginal, and right lateralorbiofrontal cortices. Compared with the controls, the schizophrenia group exhibited significantly lower ISC in the left superior temporal sulcus, bilateral supramarginal, and bilateral inferiorparietal cortices. Higher clinical severity (higher total PANSS rating) was associated with lower ISC in the middle frontal and middle temporal regions, and also higher ISC in the visual cortex, inferior temporal gyrus, and anterior cingulate. The findings indicated that patients with schizophrenia are characterized by lower ISC in a frontal parietal network while viewing comedy film clips, which implicated a deficit in the cognitive component of humor processing. The lower synchronization in parts of the frontal parietal network also correlated with symptom severity.
Interrogating Mutant Allele Expression via Customized Reference Genomes to Define Influential Cancer Mutations
Authors: Grant Adam D. , Vail Paris , Padi Megha , Witkiewicz Agnieszka K. , Knudsen Erik S. 
Published: September 2019

AbstractGenetic alterations are essential for cancer initiation and progression. However, differentiating mutations that drive the tumor phenotype from mutations that do not affect tumor fitness remains a fundamental challenge in cancer biology. To better understand the impact of a given mutation within cancer, RNA-sequencing data was used to categorize mutations based on their allelic expression. For this purpose, we developed the MAXX (Mutation Allelic Expression Extractor) software, which is highly effective at delineating the allelic expression of both single nucleotide variants and small insertions and deletions. Results from MAXX demonstrated that mutations can be separated into three groups based on their expression of the mutant allele, lack of expression from both alleles, or expression of only the wild-type allele. By taking into consideration the allelic expression patterns of genes that are mutated in PDAC, it was possible to increase the sensitivity of widely used driver mutation detection methods, as well as identify subtypes that have prognostic significance and are associated with sensitivity to select classes of therapeutic agents in cell culture. Thus, differentiating mutations based on their mutant allele expression via MAXX represents a means to parse somatic variants in tumor genomes, helping to elucidate a gene’s respective role in cancer.
The indole compound MA-35 attenuates tumorigenesis in an inflammation-induced colon cancer model
Authors: Kanehara Keigo , Ohnuma Shinobu , Kanazawa Yoshitake , Sato Keisuke , Kokubo Shoji , Suzuki Hideyuki , Karasawa Hideaki , Suzuki Takehiro , Suzuki Chitose , Naitoh Takeshi , Unno Michiaki , Abe Takaaki 
Published: September 2019

AbstractIn inflammatory bowel disease, chronic inflammation results in the development of colon cancer known as colitis-associated cancer. This disease is associated with tumor necrosis factor-α (TNF-α) signaling. In addition, intestinal fibrosis is a common clinical complication that is promoted by transforming growth factor β1 (TGF-β1). In our previous study, MA-35 attenuated renal fibrosis by inhibiting both TNF-α and TGF-β1 signaling. This study aimed to identify the possible antitumor effects and antifibrotic effects of MA-35 using an AOM/DSS mouse model. MA-35 was orally administered every day for 70 days in the AOM/DSS mouse model. There was no difference in weight loss between the AOM/DSS group and the AOMDSS + MA-35 group, but the disease activity index score and the survival rate were improved by MA-35. MA-35 blocked the anemia and shortening of the colon induced by AOM/DSS. MA-35 reduced the macroscopic formation of tumors in the colon. In the microscopic evaluation, MA-35 reduced inflammation and fibrosis in areas with dysplasia. Furthermore, the TNF-α mRNA level in the colon tended to be reduced, and the interleukin 6, TGF-β1 and fibronectin 1 mRNA levels in the colon were significantly reduced by MA-35. These results suggested that MA-35 inhibited AOM/DSS-induced carcinogenesis by reducing inflammation and fibrosis.
Critical transitions in suspended sediment dynamics in a temperate meso-tidal estuary
Authors: Cox T. J. S. , Maris T. , Van Engeland T. , Soetaert K. , Meire P. 
Published: September 2019

AbstractThere is growing consensus that human interventions can fundamentally change fine sediment transport in estuaries. Critical transitions in response to human interventions have been hypothesized based on indirect observational evidence and theoretical understanding. So far direct evidence has been lacking. Based on a 20 year data-set of surface suspended particulate matter (SPM) concentrations, we present empirical evidence of critical transitions in a temperate meso-tidal estuary. In 2008–2009 the SPM dynamics of the Scheldt estuary (Belgium/The Netherlands) changed dramatically. Not only did the total amount of sediment in suspension increase, a new maximum turbidity zone (MTZ) at typical winter discharges appeared. At intermediate and low summer discharges the longitudinal distribution of SPM now flickers between two markedly different states. Our data suggest that a range of human interventions (fairway widening and deepening, dredging and dumping activities) set the scene leading to the observed transitions. Moreover the freshwater MTZ in the Scheldt and in its major tributary exhibit an increasing sensitivity towards freshwater discharge, coinciding with water quality improvements. This suggests large scale impacts of changes in eutrophication status on estuarine sediment dynamics. This has largely been a blind spot in morphodynamic research.
The Detection and Characterization of Herpes Simplex Virus Type 1 in Confirmed Measles Cases
Authors: Li Chongshan , Li Yunyi , Yang Yuying , Wang Jing , Zhu Caixia , Tang Suwen , Pang Cong , Tang Wei , Cai Qiliang , Li Zhi , Hu Jiayu , Cui Xiaoxian , Zhang Xi , Xu Songtao , Zhang Yan , Yuan Zhengan , Hu Yunwen , Yuan Zhenghong 
Published: September 2019

AbstractBased on measles surveillance in Shanghai, People’s Republic of China, from 2006 to 2015, we found that measles virus isolates from 40 throat swab samples exhibited atypical cytopathic effects in Vero/hSLAM cells, which was found to be a result of coinfection with measles virus (MeV) and human herpes simplex virus type 1 (HSV-1). Serological and molecular approaches were used to confirm and characterize the coinfections in these patients. Among the 40 measles cases, measles-specific IgM was detected in 37 cases, while measles-specific IgG was detected in 27 cases. HSV-1-specific IgM and IgG were detected in 7 and 34 cases, respectively, suggesting that most of the MeV infections were primary, but that HSV-1 infection was due to the reactivation of latent virus in most cases. The titers of HSV-1 IgG in patients with either measles or measles-HSV-1 coinfection were significantly higher than those in the healthy group (P = 0.0026 and P < 0.0001, respectively); however, there was no significant difference in the titers of HSV-1 IgG in the MeV and MeV-HSV-1 coinfection patients (P = 0.105). Nucleic acids from MeV and HSV-1 were detected in 40 and 39 throat swabs, respectively. Twenty five MeV RNA sequences were genotyped, and all represented genotype H1, which is the endemic genotype in China. Sequences from the glycoprotein G gene of HSV-1 were used to classify the isolates into two distinct phylogenetic groups: 34 belonged to group A and 3 belonged to group B.
Sampling, identification and sensory evaluation of odors of a newborn baby’s head and amniotic fluid
Authors: Uebi Tatsuya , Hariyama Takahiko , Suzuki Kazunao , Kanayama Naohiro , Nagata Yoshifumi , Ayabe-Kanamura Saho , Yanase Shihoko , Ohtsubo Yohsuke , Ozaki Mamiko 
Published: September 2019

AbstractFor baby odor analyses, noninvasive, stress-free sample collection is important. Using a simple method, we succeeded in obtaining fresh odors from the head of five newborn babies. These odors were chemically analyzed by two-dimensional gas chromatography coupled with mass spectrometry (GC × GC-MS), and compared with each other or with the odor of amniotic fluid from the baby’s mother. We identified 31 chemical components of the volatile odors from neonate heads and 21 from amniotic fluid. Although 15 of these components were common to both sources, there was an apparent difference in the GC × GC patterns between the head and amniotic fluid odors, so the neonate head odor might be individually distinct immediately after birth. Therefore, we made artificial mixtures of the major odor components of the neonate head and maternal amniotic fluid, and used psychological tests to examine whether or not these odors could be distinguished from each other. Our data show that the artificial odor of a neonate head could be distinguished from that of amniotic fluid, and that the odors of artificial head odor mixtures could be correctly discriminated for neonates within an hour after birth and at 2 or 3 days of age.
Identification of stably expressed housekeeping miRNAs in endothelial cells and macrophages in an inflammatory setting
Authors: Link Fabian , Krohn Knut , Schumann Julia 
Published: September 2019

AbstractReliable quantification of miRNA expression by qRT-PCR crucially depends on validated housekeepers for data normalization. Here we present thoroughly tested miRNAs eligible as references in immunological studies utilizing endothelial cells and macrophages, respectively. Endothelial cells (cell line: TIME) and macrophages (cell line: RAW264.7) were treated with various pro- and anti-inflammatory mediators (cytokines, LPS, unsaturated fatty acids) given as either single substances or in combination. Isolated RNA was screened for stably expressed miRNAs by next generation sequencing. Housekeeper candidates were thereafter validated by means of two independent quantification techniques: qRT-PCR for relative quantification and ddPCR for absolute quantification. Both methods consistently confirmed the suitability of let-7g-5p, let-7i-5p, miR-127-3p and miR-151a-5p in cytokine/fatty acid-treated TIME and miR-16-5p, miR-27b-3p, miR-103a-3p and miR-423-3p in LPS/fatty acid-treated RAW264.7, respectively as housekeeping miRNAs. With respect to abundancy and over all expression stability the miRNAs miR-151a-5p (cell line: TIME) as well as miR-27b-3p and miR-103a-3p (cell line: RAW264.7) can be particularly recommended for normalization of qRT-PCR data.
Target-oriented motor imagery for grasping action: different characteristics of brain activation between kinesthetic and visual imagery
Authors: Lee Woo Hyung , Kim Eunkyung , Seo Han Gil , Oh Byung-Mo , Nam Hyung Seok , Kim Yoon Jae , Lee Hyun Haeng , Kang Min-Gu , Kim Sungwan , Bang Moon Suk 
Published: September 2019

AbstractMotor imagery (MI) for target-oriented movements, which is a basis for functional activities of daily living, can be more appropriate than non-target-oriented MI as tasks to promote motor recovery or brain-computer interface (BCI) applications. This study aimed to explore different characteristics of brain activation among target-oriented kinesthetic imagery (KI) and visual imagery (VI) in the first-person (VI-1) and third-person (VI-3) perspectives. Eighteen healthy volunteers were evaluated for MI ability, trained for the three types of target-oriented MIs, and scanned using 3 T functional magnetic resonance imaging (fMRI) under MI and perceptual control conditions, presented in a block design. Post-experimental questionnaires were administered after fMRI. Common brain regions activated during the three types of MI were the left premotor area and inferior parietal lobule, irrespective of the MI modalities or perspectives. Contrast analyses showed significantly increased brain activation only in the contrast of KI versus VI-1 and KI versus VI-3 for considerably extensive brain regions, including the supplementary motor area and insula. Neural activity in the orbitofrontal cortex and cerebellum during VI-1 and KI was significantly correlated with MI ability measured by mental chronometry and a self-reported questionnaire, respectively. These results can provide a basis in developing MI-based protocols for neurorehabilitation to improve motor recovery and BCI training in severely paralyzed individuals.
Magnetohydrodynamic nanofluid radiative thermal behavior by means of Darcy law inside a porous media
Authors: Nguyen-Thoi Trung , Sheikholeslami M. , Shah Zahir , Kumam Poom , Shafee Ahmad 
Published: September 2019

AbstractRadiative nanomaterial thermal behavior within a permeable closed zone with elliptic hot source is simulated. Darcy law is selected for simulating permeable media in existence of magnetic forces. Contour plots for various buoyancy, Hartmann numbers and radiation parameter were illustrated. Carrier fluid is Al2O3-water with different shapes. Outputs prove that conduction mode augments with enhance ofHa.Nuaugments with considering radiation source term.
Inkjet-printed MXene micro-scale devices for integrated broadband ultrafast photonics
Authors: Jiang Xiantao , Li Wenjia , Hai Ting , Yue Rui , Chen Zhangwei , Lao Changshi , Ge Yanqi , Xie Guoqiang , Wen Qiao , Zhang Han 
Published: September 2019

AbstractMXene, as a novel 2D crystal material, possessing tunable bandgap, low optical attenuation and broadband nonlinear optical responses that may promote the fabrications of advanced electro-photonics devices has gathered remarkable attention recently. However, current investigations of 2D crystals for photonics devices suffer from the limitations of reproducibility, scalability, and compatibility. Inkjet printing is one of the powerful additive manufacturers that facilitate well-controlled, low-cost, scalable and small-footprint electro-photonics devices on myriad substrates. Herein, we directly inkjet printed MXene nanosheets in laser resonators with both fiber and free-space geometrics, and achieved extensive spectral band ultrafast laser operations from near- to the mid-infrared regime with pulse duration going to 100 femtoseconds. The demonstrations of versatile inkjet-printed devices based on MXene, while forthputting its distinct electro-optical properties, may allow the realizations of advanced MXene enable photonics devices shortly.
Structural determinants of CO2-sensitivity in the β connexin family suggested by evolutionary analysis
Authors: Dospinescu Valentin-Mihai , Nijjar Sarbjit , Spanos Fokion , Cook Jonathan , de Wolf Elizabeth , Biscotti Maria Assunta , Gerdol Marco , Dale Nicholas 
Published: September 2019

AbstractA subclade of connexins comprising Cx26, Cx30, and Cx32 are directly sensitive to CO2. CO2 binds to a carbamylation motif present in these connexins and causes their hemichannels to open. Cx26 may contribute to CO2-dependent regulation of breathing in mammals. Here, we show that the carbamylation motif occurs in a wide range of non-mammalian vertebrates and was likely present in the ancestor of all gnathostomes. While the carbamylation motif is essential for connexin CO2-sensitivity, it is not sufficient. In Cx26 of amphibia and lungfish, an extended C-terminal tail prevents CO2-evoked hemichannel opening despite the presence of the motif. Although Cx32 has a long C-terminal tail, Cx32 hemichannels open to CO2 because the tail is conformationally restricted by the presence of proline residues. The loss of the C-terminal tail of Cx26 in amniotes was an evolutionary innovation that created a connexin hemichannel with CO2-sensing properties suitable for the regulation of breathing.
Response mechanism of mine-isolated fungus Aspergillus niger IOC 4687 to copper stress determined by proteomics
Authors: Dias Meriellen , Gomes de Lacerda José Thalles Jocelino , Perdigão Cota de Almeida Silas , de Andrade Lidiane Maria , Oller do Nascimento Claudio Augusto , Rozas Enrique Eduardo , Mendes Maria Anita 
Published: September 2019

Abstract
Proteomic analysis of the fungus Aspergillus niger showed that its capacity to absorb metals was boosted by physiological modification under metal stress conditions. To investigate the proteome elicited by copper stress, the mine-isolated strain A. niger IOC 4687 was cultured in the absence (control) or presence of copper ions (50 mg L−1) for 72 h. Protein extract from each treatment was analyzed by nano-liquid chromatography-mass spectrometry and proteins were identified using PEAKS Studio 8.5 software. Grouping proteins by functional category showed that antioxidant enzymes, such as catalase, superoxide dismutase, and cytochrome c peroxidase, were present in both treatments. However, heat shock proteins (Hsp60 and Hsp70) and some metalloproteins (LMBR1 domain protein and A. niger contig An09c0040) were only observed after copper treatment. These proteins were the cellular response to the stress conditions. In conclusion, significant changes in the proteome of A. niger were observed due to the presence of copper.