bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adeno-associated virus Rep proteins antagonize phosphatase PP1 to counteract KAP1 repression of the latent viral genome
Authors: Smith-Moore Sarah , Neil Stuart J. D. , Fraefel Cornel , Linden R. Michael , Bollen Mathieu , Rowe Helen M. , Henckaerts Els 
Published: March 2018

Significance
In recent years, adeno-associated virus (AAV) has attracted considerable attention as a result of its success as a gene therapy vector. However, several aspects of its biology remain elusive. Given that AAV vectors mimic the latent phase of the viral life cycle, defining the mechanisms involved in the regulation of AAV latency is of particular importance. Our studies demonstrate that epigenetic processes are involved in the regulation of viral latency and reveal virus–host interactions and helper functions that are aimed at counteracting the epigenetic repression of the viral genome during the lytic phase of the viral life cycle. These observations will inform the design of future AAV vector technologies.
Fusogenic micropeptide Myomixer is essential for satellite cell fusion and muscle regeneration
Authors: Bi Pengpeng , McAnally John R. , Shelton John M. , Sánchez-Ortiz Efrain , Bassel-Duby Rhonda , Olson Eric N. 
Published: March 2018

Significance
Skeletal muscle damaged by injury or disease can regenerate new muscle fibers. The regenerative properties of skeletal muscle involve fusion of activated muscle stem cells (satellite cells). We recently discovered Myomixer, a conserved micropeptide that is specifically expressed during muscle formation. Myomixer, together with its partner Myomaker, another muscle-specific membrane protein, is necessary for muscle formation during embryogenesis. Here, we show the absolute requirement of Myomixer for the fusion of satellite cells and regeneration of adult muscle in response to injury. Our findings provide insights into the mechanisms of muscle formation and suggest opportunities for enhancing muscle regeneration through manipulation of Myomixer and Myomaker.
Early Modern Comedy and the Politics of Religion: Reconsidering Comedy in Rospigliosi's Il Sant'Alessio
Authors: Purciello Maria Anne 
Published: March 2018

ABSTRACTGiulio Rospigliosi and Stefano Landi's 1634 revisions of Il Sant'Alessio for the Barberini stage expanded the role of comedy within the opera. These revisions reveal an important juncture in the history of religious comedy and lay the foundation for the development of a comic rubric for the still-developing operatic genre. This article examines the legend of St Alexis and its potential for historic reinterpretation in light of the Barberini family's religious and political goals. Its consideration of the interrelationship of religion and comedy in early modern sacred drama provides the theoretical context for an analysis of scenes in the 1634 version featuring the comic pages Martio and Curtio. Operating as a mitigating force between the extreme ideologies presented by the characters of Sant'Alessio and the Devil, the pages’ comedy promotes a more widespread acceptance of key religious ideals and becomes the driving force behind a significant political statement for post-Tridentine Rome.
Utilizing Risk Index for Overdose Or Serious Opioid-Induced Respiratory Depression (RIOSORD) Scores to Prioritize Offer of Rescue Naloxone in An Outpatient Veteran Population: A Telephone-Based Project
Authors: Yates Derek , Frey Theresa , Montgomery Jean Charles 
Published: March 2018

Background Since 2014, the Department of Veterans Affairs (VA) has been working to address the ongoing opioid epidemic through opioid-education initiatives, the development of risk calculators, and other risk stratification tools. One primary focus of VA efforts has been the distribution of rescue naloxone kits to veterans at greatest risk of opioid-related adverse events. The purpose of this project was to identify primary care veterans at highest risk for serious opioid-related adverse events using the Risk Index for Overdose and Serious Opioid-Induced Respiratory Depression (RIOSORD) and offer rescue naloxone kits by telephone-based outreach. RIOSORD is a risk-stratification tool developed and validated within the veteran population. Methods Veterans identified at highest risk of overdose or opioid-related adverse effects were contacted by telephone or letter to offer to provide a rescue naloxone kit between November 1st, 2016 and February 1st, 2017. The primary outcome of the project was the percentage of patients contacted that agreed to a naloxone prescription. Secondary outcomes included comparative efficacy of phone versus letter contact and reasons for refusal of naloxone if the offer was declined. Results Of 41 veterans targeted by this project, most were successfully reached by telephone within three attempts (92.7%, n = 38). Approximately two-thirds of those reached by telephone agreed to a prescription for rescue naloxone (n = 26, 63.4%). The veterans that requested rescue naloxone selected the nasal formulation (n = 17) over the intramuscular auto-injector (n = 9). Conclusions This project demonstrated that telephone-based outreach can be one method of distributing rescue naloxone to a high-risk patient population without requiring an in-person visit to a provider.
Hypertrophic Scar Severity at Autograft Sites Is Associated With Increased Pain and Itch After Major Thermal Burn Injury
Authors: Mauck Matthew C , Shupp Jeffrey W , Williams Felicia , Villard Marie Ashley , Jones Samuel W , Hwang James , Smith Jennifer , Karlnoski Rachel , Smith David J , Cairns Bruce A , McLean Samuel A 
Published: March 2018

Abstract
Approximately three quarters of major thermal burn injury (MThBI) survivors suffer from hypertrophic scarring (HTS) and over half experience chronic pain or itch. In survivors of MThBI, HTS and chronic pain or itch are considered one of the greatest unmet challenges of postburn injury care and psychosocial reintegration. Although scarring, itch, and pain have been clinically associated, there are no prospective, multisite studies examining tissue autograft site pain or itch and scar outcomes. The authors collected a representative cohort (n = 56) of MThBI survivors who received autografting within 14 days of injury and evaluated graft-site pain or itch severity (0–10 Numeric Rating Scale) and HTS using a validated scar photograph assessment scale 6 months following MThBI. Given that stress is known to influence wound healing, the authors also assessed the relationship between previous trauma exposure, peritraumatic stress, preburn overall health (SF-12), scarring, and chronic pain or itch severity using Spearman’s correlation. Association between HTS and chronic pain or itch was significant in a linear regression model adjusted for age, sex, and ethnicity (β = 0.2, P = .033 for pain, β = 0.2, P = .019 for itch). Results indicate that prior trauma exposure is inversely correlated (r = −.363, P = .030) with scar severity, but not pain or itch severity 6 months after MThBI. Study results suggest that preburn chronic pain or itch is associated with pathological scarring 6 months following MThBI. Results also indicate that stress may improve scarring after MThBI. Further work to understand the mechanisms that underlie both HTS and chronic pain or itch and their relationship to chronic stress is critical to the development of novel therapies to assist burn survivors recover.
Cytochrome P450 2B diversity in a dietary specialist—the red tree vole (Arborimus longicaudus)
Authors: Kitanovic Smiljka , Marks-Fife Chad A , Parkes Quincy A , Wilderman P Ross , Halpert James R , Dearing M Denise 
Published: March 2018

AbstractAlthough herbivores rely on liver enzymes to biotransform plant secondary metabolites ingested in plant-based diets, only a few enzymes from a handful of species have been characterized at the genomic level. In this study, we examined cytochrome P450 2B (CYP2B) sequence diversity and gene copy number in a conifer specialist, the red tree vole (Arborimus longicaudus). We fed captive individuals exclusively Douglas-fir (Pseudotsuga menziesii) foliage, cloned and sequenced their liver CYP2B cDNA, and estimated CYP2B gene copy number. We identified 21 unique CYP2B nucleotide sequences, and 20 unique CYP2B amino acid sequences. Gene copy number of CYP2B was estimated at 7.7 copies per haploid genome. We compared red tree vole CYP2B with CYP2B sequences of a generalist, the prairie vole (Microtus ochrogaster), found in GenBank. Our study revealed that the CYP2B enzymes of red tree voles possess unique sequences compared to CYP2B enzymes of other herbivorous species. The unique combination of amino acid residues at key substrate recognition sites of CYP2B enzymes may underlie the ability of the red tree vole to specialize on a highly toxic diet of Douglas-fir.
Tumor Specimen Biobanks: Data Gaps for Analyzing Health Inequities—the Case of Breast Cancer
Authors: Krieger Nancy , Jahn Jaquelyn L 
Published: March 2018

Abstract
Biobanks are increasingly recognized to be vital for analyzing tumor properties, treatment options, and clinical prognosis, yet few data exist on whether they are equipped to enable research on cancer inequities, that is, unfair and unnecessary social group differences in health. We conducted a systematic search of global biobanks, identified 46 that have breast tumor tissue and share data externally with academic researchers, and e-mailed and called to obtain data on the sociodemographic, socioeconomic, and geospatial data included, plus time span encompassed. Among the 32 biobank respondents, 91% housed specimens solely from the Global North, only 31% obtained socioeconomic data, 63% included racial/ethnic data (of which 55% lacked socioeconomic data), 44% included limited geographic data, and 55% had specimens dating back at most to 2000. To enable research to address cancer inequities, including trends over time, biobanks will need to address the data gaps documented by our study.
Constraining hydrostatic mass bias of galaxy clusters with high-resolution X-ray spectroscopy
Authors: Ota Naomi , Nagai Daisuke , Lau Erwin T 
Published: March 2018

Abstract
Gas motions in galaxy clusters play important roles in determining the properties of the intracluster medium (ICM) and in the constraint of cosmological parameters via X-ray and Sunyaev–Zel’dovich effect observations of galaxy clusters. The Hitomi measurements of gas motions in the core of the Perseus Cluster have provided new insights into the physics in galaxy clusters. The XARM mission, equipped with the Resolve X-ray micro-calorimeter, will continue Hitomi’s legacy by measuring ICM motions through Doppler shifting and broadening of emission lines in a larger number of galaxy clusters, and at larger radii. In this work, we investigate how well we can measure bulk and turbulent gas motions in the ICM with XARM, by analyzing mock XARM simulations of galaxy clusters extracted from cosmological hydrodynamic simulations. We assess how photon counts, spectral fitting methods, multiphase ICM structure, deprojections, and region selection affect the measurements of gas motions. We first show that XARM is capable of recovering the underlying spherically averaged turbulent and bulk velocity profiles for dynamically relaxed clusters to within ∼50% with a reasonable amount of photon counts in the X-ray emission lines. We also find that there are considerable azimuthal variations in the ICM velocities, where the velocities measured in a single azimuthal direction can significantly deviate from the true value even in dynamically relaxed systems. Such variation must be taken into account when interpreting data and developing observing strategies. We will discuss the prospect of using the upcoming XARM mission to measure non-thermal pressure and to correct for the hydrostatic mass bias of galaxy clusters. Our results are broadly applicable for future X-ray missions, such as Athena and Lynx.
Comparison of the Efficacy and Efficiency of the Use of Virtual Reality Simulation With High-Fidelity Mannequins for Simulation-Based Training of Fiberoptic Bronchoscope Manipulation
Authors: Jiang Bailin , Ju Hui , Zhao Ying , Yao Lan , Feng Yi 
Published: March 2018

Introduction
This study compared the efficacy and efficiency of virtual reality simulation (VRS) with high-fidelity mannequin in the simulation-based training of fiberoptic bronchoscope manipulation in novices.

Methods
Forty-six anesthesia residents with no experience in fiberoptic intubation were divided into two groups: VRS (group VRS) and mannequin (group M). After a standard didactic teaching session, group VRS trained 25 times on VRS, whereas group M performed the same process on a mannequin. After training, participants' performance was assessed on a mannequin five consecutive times. Procedure times during training were recorded as pooled data to construct learning curves. Procedure time and global rating scale scores of manipulation ability were compared between groups, as well as changes in participants' confidence after training.

Results
Plateaus in the learning curves were achieved after 19 (95% confidence interval = 15–26) practice sessions in group VRS and 24 (95% confidence interval = 20–32) in group M. There was no significant difference in procedure time [13.7 (6.6) vs. 11.9 (4.1) seconds, t′ = 1.101, P = 0.278] or global rating scale [3.9 (0.4) vs. 3.8 (0.4), t = 0.791, P = 0.433] between groups. Participants' confidence increased after training [group VRS: 1.8 (0.7) vs. 3.9 (0.8), t = 8.321, P < 0.001; group M = 2.0 (0.7) vs. 4.0 (0.6), t = 13.948, P < 0.001] but did not differ significantly between groups.

Conclusions
Virtual reality simulation is more efficient than mannequin in simulation-based training of flexible fiberoptic manipulation in novices, but similar effects can be achieved in both modalities after adequate training.
Collective movement in ecology: from emerging technologies to conservation and management
Authors: Westley Peter A. H. , Berdahl Andrew M. , Torney Colin J. , Biro Dora 
Published: March 2018

Recent advances in technology and quantitative methods have led to the emergence of a new field of study that stands to link insights of researchers from two closely related, but often disconnected disciplines: movement ecology and collective animal behaviour. To date, the field of movement ecology has focused on elucidating the internal and external drivers of animal movement and the influence of movement on broader ecological processes. Typically, tracking and/or remote sensing technology is employed to study individual animals in natural conditions. By contrast, the field of collective behaviour has quantified the significant role social interactions play in the decision-making of animals within groups and, to date, has predominantly relied on controlled laboratory-based studies and theoretical models owing to the constraints of studying interacting animals in the field. This themed issue is intended to formalize the burgeoning field of collective movement ecology which integrates research from both movement ecology and collective behaviour. In this introductory paper, we set the stage for the issue by briefly examining the approaches and current status of research in these areas. Next, we outline the structure of the theme issue and describe the obstacles collective movement researchers face, from data acquisition in the field to analysis and problems of scale, and highlight the key contributions of the assembled papers. We finish by presenting research that links individual and broad-scale ecological and evolutionary processes to collective movement, and finally relate these concepts to emerging challenges for the management and conservation of animals on the move in a world that is increasingly impacted by human activity.This article is part of the theme issue ‘Collective movement ecology’.
The importance of individual variation in the dynamics of animal collective movements
Authors: del Mar Delgado Maria , Miranda Maria , Alvarez Silvia J. , Gurarie Eliezer , Fagan William F. , Penteriani Vincenzo , di Virgilio Agustina , Morales Juan Manuel 
Published: March 2018

Animal collective movements are a key example of a system that links two clearly defined levels of organization: the individual and the group. Most models investigating collective movements have generated coherent collective behaviours without the inclusion of individual variability. However, new individual-based models, together with emerging empirical information, emphasize that within-group heterogeneity may strongly influence collective movement behaviour. Here we (i) review the empirical evidence for individual variation in animal collective movements, (ii) explore how theoretical investigations have represented individual heterogeneity when modelling collective movements and (iii) present a model to show how within-group heterogeneity influences the collective properties of a group. Our review underscores the need to consider variability at the level of the individual to improve our understanding of how individual decision rules lead to emergent movement patterns, and also to yield better quantitative predictions of collective behaviour.
This article is part of the theme issue ‘Collective movement ecology’.
Friendship across species borders: factors that facilitate and constrain heterospecific sociality
Authors: Sridhar Hari , Guttal Vishwesha 
Published: March 2018

Our understanding of animal sociality is based almost entirely on single-species sociality. Heterospecific sociality, although documented in numerous taxa and contexts, remains at the margins of sociality research and is rarely investigated in conjunction with single-species sociality. This could be because heterospecific and single-species sociality are thought to be based on fundamentally different mechanisms. However, our literature survey shows that heterospecific sociality based on mechanisms similar to single-species sociality is reported from many taxa, contexts and for various benefits. Therefore, we propose a conceptual framework to understand conspecific versus heterospecific social partner choice. Previous attempts, which are all in the context of social information, model partner choice as a trade-off between information benefit and competition cost, along a single phenotypic distance axis. Our framework of partner choice considers both direct grouping benefits and information benefits, allows heterospecific and conspecific partners to differ in degree and qualitatively, and uses a multi-dimensional trait space analysis of costs (competition and activity matching) and benefits (relevance of partner and quality of partner). We conclude that social partner choice is best-viewed as a continuum: some social benefits are obtainable only from conspecifics, some only from dissimilar heterospecifics, while many are potentially obtainable from conspecifics and heterospecifics.This article is part of the theme issue ‘Collective movement ecology'.
The genetic basis of the human-cannabis relationship
Authors: Henry Philippe 
Published: March 2018

AbstractCannabis can elicit various reactions in different consumers. In order to shed light on the mechanisms underlying the human-cannabis relationship, we begin to investigate the genetic basis of this differential response. The web-based platform OpenSNP was used to collect selfreported genetic and phenotypic data. Participants either reported a positively or negative affinity to cannabis. A total of 26 individuals were retained, 10 of which indicated several negative responses and the remaining 16 indicating strong affinity for Cannabis. A total of 325’895 single nucleotide polymorphisms (SNPs) were retained. The software TASSEL 5 was used to run a genome-wide association study (GWAS), with a generalized liner model (GLM) and1000 permutations. The analysis yielded a set of 45 SNPs that were significantly associated with the reported affinity to cannabis, including one strong outlier found in the MYO16 gene. A diagnostic process is proposed by which individuals can be assessed for their affinity to cannabis. We believe this type of tool may be helpful in alleviating some of the stigma associated with cannabis use in individuals sensitive to THC and other cannabis constituents such as myrcene, which may potentiate negative responses.
Tendency towards being a “Morning person” increases risk of Parkinson’s disease: evidence from Mendelian randomisation
Authors: Noyce AJ , Kia DA , Heilbron K , Jepson JEC , Hemani G ,   The International Parkinson’s Disease Genomics Consortium,   The 23andMe Research Team, Hinds DA , Lawlor DA , Davey Smith G , Hardy J , Singleton A , Nalls MA , Wood NW 
Published: March 2018

AbstractBackgroundCircadian rhythm may play a role in neurodegenerative diseases such as Parkinson’s disease (PD). Chronotype is the behavioural manifestation of circadian rhythm and Mendelian randomisation (MR) involves the use of genetic variants to explore causal effects of exposures on outcomes. This study aimed to explore a causal relationship between chronotype and coffee consumption on risk of PD.MethodsTwo-sample MR was undertaken using publicly available GWAS data. Associations between genetic instrumental variables (IV) and “morning person” (one extreme of chronotype) were obtained from the personal genetics company 23andMe, Inc., and UK Biobank, and consisted of the per-allele odds ratio of being a “morning person” for 15 independent variants. The per-allele difference in log-odds of PD for each variant was estimated from a recent meta-analysis. The inverse variance weight method was used to estimate an odds ratio (OR) for the effect of being a “morning person” on PD. Additional MR methods were used to check for bias in the IVW estimate, arising through violation of MR assumptions. The results were compared to analyses employing a genetic instrument of coffee consumption, because coffee consumption has been previously inversely linked to PD.FindingsBeing a “morning person” was causally linked with risk of PD (OR 1⋅27; 95% confidence interval 1⋅06-1⋅51; p=0⋅012). Sensitivity analyses did not suggest that invalid instruments were biasing the effect estimate and there was no evidence for a reverse causal relationship between liability for PD and chronotype. There was no robust evidence for a causal effect of high coffee consumption using IV analysis, but the effect was imprecisely estimated (OR 1⋅12; 95% CI 0⋅89-1⋅42; p=0⋅22).InterpretationWe observed causal evidence to support the notion that being a “morning person”, a phenotype driven by the circadian clock, is associated with a higher risk of PD. Further work on the mechanisms is warranted and may lead to novel therapeutic targets.FundingNo specific funding source.