bluuebunny/crossref_metadata_2025_split huggingface dataset:
Benefits of stable isotope labeling in RNA analysis
Authors: Asadi-Atoi Paria , Barraud Pierre , Tisne Carine , Kellner Stefanie 
Published: March 2019

AbstractRNAs are key players in life as they connect the genetic code (DNA) with all cellular processes dominated by proteins. They contain a variety of chemical modifications and many RNAs fold into complex structures. Here, we review recent progress in the analysis of RNA modification and structure on the basis of stable isotope labeling techniques. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy are the key tools and many breakthrough developments were made possible by the analysis of stable isotope labeled RNA. Therefore, we discuss current stable isotope labeling techniques such as metabolic labeling, enzymatic labeling and chemical synthesis. RNA structure analysis by NMR is challenging due to two major problems that become even more salient when the size of the RNA increases, namely chemical shift overlaps and line broadening leading to complete signal loss. Several isotope labeling strategies have been developed to provide solutions to these major issues, such as deuteration, segmental isotope labeling or site-specific labeling. Quantification of modified nucleosides in RNA by MS is only possible through the application of stable isotope labeled internal standards. With nucleic acid isotope labeling coupled mass spectrometry (NAIL-MS), it is now possible to analyze the dynamic processes of post-transcriptional RNA modification and demodification. The trend, in both NMR and MS RNA analytics, is without doubt shifting from the analysis of snapshot moments towards the development and application of tools capable of analyzing the dynamics of RNA structure and modification profiles.
The Gid-complex: an emerging player in the ubiquitin ligase league
Authors: Liu Huaize , Pfirrmann Thorsten 
Published: March 2019

Abstract
The Saccharomyces cerevisiae Gid-complex is a highly evolutionary conserved ubiquitin ligase with at least seven protein subunits. Here, we review our knowledge about the yeast Gid-complex as an important regulator of glucose metabolism, specifically targeting key enzymes of gluconeogenesis for degradation. Furthermore, we summarize existing data about the individual subunits, the topology and possible substrate recognition mechanisms and compare the striking similarities, but also differences, between the yeast complex and its vertebrate counterpart. Present data is summarized to give an overview about cellular processes regulated by the vertebrate GID-complex that range from cell cycle regulation, primary cilia function to the regulation of energy homeostasis. In conclusion, the vertebrate GID-complex evolved as a versatile ubiquitin ligase complex with functions beyond the regulation of glucose metabolism.
Optimal Energy Scheduling Method under Load Shaping Demand Response Program in a Home Energy Management System
Authors: Kakran Sandeep , Chanana Saurabh 
Published: March 2019

Abstract
With the latest smart technologies in the electricity sector, the consumers of electricity got the opportunity to reduce their electricity consumption cost by participating in the demand response programs offered by the utility companies. In this paper, a model of energy management system is introduced for the energy scheduling at home. Residential automatic smart appliances of general use are selected for energy scheduling. The energy controlling device in the EMS model receives the real time electricity price signals from the utility company and schedule the appliances according to the user requirements in such a way so that the electricity consumption cost could be minimized. The appliances are scheduled under real time pricing combined with inclined block rate pricing scheme so that the peak to average ratio of power could be maintained in the satisfactory range. This helps the utility companies in maintaining the system reliability. For the solution of the scheduling problem, particle swarm optimization algorithm is used due to its effectiveness and easy implementation. Finally, the results have been compared and verified against the results achieved by genetic algorithm.
Electronic Instrumentation and Computational Simulation to Evaluate the Combined Use of Microwave and Infrared Technologies for Reheating Biphasic Foods
Authors: Piza Luciana Vieira , Von Atzingen Gustavo Voltani , Costa Ernane José Xavier 
Published: March 2019

Abstract
Combined use of microwave and infrared technologies for reheating food may be considered an efficient technique in food industry and fast food chains, as the overall quality and identity standard are maintained. The aim of this study was to test a new methodology for reheating baked foods, using both radiations. Computer simulation and preliminary tests were carried out controlling radiations emissions. The tests were conducted with biphasic foods to show the effectiveness and action of technologies. Preliminary studies were carried out to verify the effect of radiations in each food phase, which provided the action times of 15 s of microwave and 30 s of infrared. It was possible to measure the internal and external temperature of the samples, as well as moisture migration from the center to the surface. The results showed that the process was effective to reheat samples, providing attractive samples to consumers, according to sensorial analysis.
Skin pathologies in pregnancy
Authors: Snarskaya Elena Sergeevna , Olisova Olga Yurievna , Makatsariya Alexander Davidovich , Kochergin Nikolai Georgievich , Radetskaya Lyudmila , Bitsadze Viktoriya , Khizroeva Jamilya 
Published: March 2019

Abstract
Progesterone is a hormone responsible for pregnancy maintenance and the amount of progesterone increases in a woman’s body during pregnancy, as well as the level of female sex hormones, estrogens are also upregulated. Due to these changes the cutaneous sensitivity to external stimuli (meteorological factors, bacteria, etc.) increases. In general, all skin changes during pregnancy can be divided into three groups: physiological changes (hormone-associated), nonspecific or dermatoses that existed before pregnancy or were triggered by it, and specific pregnancy-related dermatoses, which appear during pregnancy and resolve in the postpartum period. In this brief  review, we describe the dermatoses commonly seen in pregnancy and present our own clinical examples. We hope the review will be of some practical help for dermatologists and obstetricians.
Peirce on facts, propositions, and the index
Authors: Atkins Richard Kenneth 
Published: March 2019

Abstract
Peirce holds that our logic should be the basis for our metaphysics. He also thinks that facts and propositions are structurally isomorphic. However, unlike many theorists who take propositions such as snow is white and grass is green as their paradigmatic examples, Peirce takes it rains (Latin: pleurit) and similar propositions as his paradigmatic examples. I explore how his analysis of such propositions and the way in which they convey meaning becomes more complex from 1895 to 1909, how this impacts his metaphysics, and how he can claim that something like the common environment of two interlocutors can itself be an index.
High-pressure synthesis and crystal structure of the mixed-cation borate Ga4In4B15O33(OH)3
Authors: Vitzthum Daniela , Huppertz Hubert 
Published: March 2019

Abstract
The mixed cation triel borate Ga4In4B15O33(OH)3 was synthesized in a Walker-type multianvil apparatus at high-pressure/high-temperature conditions of 12.5 GPa and 1300°C. Although the product could not be reproduced in further experiments, its crystal structure could be reliably determined via single-crystal X-ray diffraction data. Ga4In4B15O33(OH)3 crystallizes in the tetragonal space group I41/a (origin choice 2) with the lattice parameters a = 11.382(2), c = 15.244(2) Å, and V = 1974.9(4) Å3. The structure of the quaternary triel borate consists of a complex network of BO4 tetrahedra, edge-sharing InO6 octahedra in dinuclear units, and very dense edge-sharing GaO6 octahedra in tetranuclear units.
Analysis of Selected Lymphocyte (CD45+) Subset Distribution in Capillary Blood of Young Soccer Players
Authors: Nowak Robert , Kostrzewa-Nowak Dorota , Buryta Rafał 
Published: March 2019

Abstract
Nowak, R, Kostrzerwa-Nowak, D, and Buryta, R. Analysis of selected lymphocyte (CD45+) subset distribution in capillary blood of young soccer players. J Strength Cond Res 35(8): 2279–2286, 2021—Mechanisms responsible for increasing athletes' physical capacity and induction of exercise-induced immunosuppression processes are not fully understood. The aim of the study was to monitor changes in percentages of lymphocyte subsets: T, Th, Tc, B, and NK cells in capillary blood of junior soccer players. Ten subjects median aged 18 years (range 17–19 years) were recruited form young soccer players. Capillary blood was collected 24 hours after each soccer match during the 8 weeks of the final phase of Central Junior League competition, and white blood cell (WBC) phenotyping was performed to determine the percentages of B lymphocytes, NK cells, and T-lymphocyte subsets. Cumulative match-time (a sum of time spend playing the game by each athlete during the observation period) was also calculated. Significant changes in the percentage of total lymphocytes (p = 0.00005) and T cells (p = 0.00006) were observed. The slight increases in lymphocytes' and Th cells' median percentages correlated with increasing cumulative match-time of studied subjects, although the correlation was not strong (R = 0.24; p = 0.0205 and R = 0.30; p = 0.0035, for lymphocytes and Th cells, respectively). It seems that the exercise bouts are among considerable factors influencing the changes in WBC subsets, especially in CD3+ cells, among young soccer players. Regarding the number of games played and training loads, they are more susceptible to immunosuppression and subsequent infections and thus should be monitored regarding WBC phenotype assessment.
Using an Inertial Device (WIMU PRO) to Quantify Neuromuscular Load in Running: Reliability, Convergent Validity, and Influence of Type of Surface and Device Location
Authors: Gómez-Carmona Carlos D. , Bastida-Castillo Alejandro , González-Custodio Adrián , Olcina Guillermo , Pino-Ortega José 
Published: March 2019

ABSTRACT
Gómez-Carmona, CD, Bastida-Castillo, A, González-Custodio, A, Olcina, G, and Pino-Ortega, J. Using an inertial device (WIMU PRO) to quantify neuromuscular load in running: reliability, convergent validity, and influence of type of surface and device location. J Strength Cond Res 34(2): 365–373, 2020—Currently, the use of accelerometers in sport is increasing, and thus, the devices are required to be valid and reliable. This study tested (a) the reliability and validity of WIMU PRO accelerometers to measure PlayerLoad (PL) and (b) the influence of speed, inertial device location, and type of surface where the incremental test is performed. Twenty resistance-trained men (age: 27.32 ± 6.65 years; height: 1.74 ± 0.03 m; body mass: 68.96 ± 4.37 kg; and body mass index: 22.76 ± 1.11 kg·m−2) volunteered to participate in the study that lasted 5 weeks. Four progressive incremental tests were performed in treadmill and athletic track conditions. External load variable (PL) and physiological variables (heart rate [HR] and SmO2) were recorded by 4 WIMU PRO inertial devices (scapulae, center of mass, knee, and ankle), a GARMIN HR band, and a MOXY near-infrared spectroscopy device, respectively. High reliability was found on both types of surface, showing the best values at the ankle (treadmill: intraclass correlation coefficient [ICC] = 0.99, coefficient of variation [CV] = 4.65%; track: ICC = 0.96, CV = 6.54%). A nearly perfect convergent validity was shown with HRAVG (r = 0.99) and a moderate one with SmO2 (r = −0.69). Significant differences in the PL variable between surfaces were reported in all locations except the scapulae (p = 0.173), and the higher values were found on the track. In the analysis per location, the ankle location reported the highest values at all speeds and on the 2 surfaces analyzed. Assessment needs to be individualized, due to the great variability of gait biomechanics among subjects. The accelerometer location should be chosen according to the purpose of the measurement, with the ankle location being recommended for neuromuscular load analysis in running.
Short-Term Delayed Effects of Kinesio Taping on Sprint Cycling Performance
Authors: Trecroci Athos , Formenti Damiano , Rossi Alessio , Esposito Fabio , Alberti Giampietro 
Published: March 2019

Abstract
Trecroci, A, Formenti, D, Rossi, A, Esposito, F, and Alberti, G. Short-term delayed effects of Kinesio taping on sprint cycling performance. J Strength Cond Res 33(5): 1232–1236, 2019—The aim of this study was to assess short-term delayed effects of Kinesio taping (KT) on maximal cycling performance. A design with repeated measures was used. Fifteen healthy trained subjects underwent 3 conditions: no KT (without tape), KT (Kinesio tape applied longitudinally on the thighs), and sham taping (Kinesio tape applied horizontally on the thighs). Each subject performed 2 sets of 3 × 6-second sprints (separated by 3 minutes) interspersed by 30 minutes of rest on a cycle ergometer. Allometric scaling of peak power (PP) and average power (AP) values were computed and analyzed for each sprint performance. Both PP and AP decreased significantly after 30 minutes (between set 1 and set 2) in all conditions, with a greater decrease in no KT condition compared with KT and sham (p < 0.01). No significant differences were observed between KT and sham conditions. The application of KT on thigh muscles attenuated the performance decrease that occurred after 30 minutes of rest between the 2 sets compared with the no KT condition. This finding suggests athletes may use KT to better manage their performance during delays in competition events.
HNCcorr: A Novel Combinatorial Approach for Cell Identification in Calcium-Imaging Movies
Authors: Spaen Quico , Asín-Achá Roberto , Chettih Selmaan N. , Minderer Matthias , Harvey Christopher , Hochbaum Dorit S. 
Published: March 2019

AbstractCalcium imaging is a key method in neuroscience for investigating patterns of neuronal activityin vivo. Still, existing algorithms to detect and extract activity signals from calcium-imaging movies have major shortcomings. We introduce the HNCcorr algorithm for cell identification in calcium-imaging datasets that addresses these shortcomings. HNCcorr relies on the combinatorial clustering problem HNC (Hochbaum’s Normalized Cut), which is similar to the Normalized Cut problem of Shi and Malik, a well known problem in image segmentation. HNC identifies cells as coherent clusters of pixels that are highly distinct from the remaining pixels. HNCcorr guarantees a globally optimal solution to the underlying optimization problem as well as minimal dependence on initialization techniques. HNCcorr also uses a new method, called “similarity squared”, for measuring similarity between pixels in calcium-imaging movies. The effectiveness of HNCcorr is demonstrated by its top performance on the Neurofinder cell identification benchmark. We believe HNCcorr is an important addition to the toolbox for analysis of calcium-imaging movies.
FosbInduction in Nucleus Accumbens by Cocaine Is Regulated by E2F3a
Authors: Cates Hannah M. , Lardner Casey K. , Bagot Rosemary C. , Neve Rachael L. , Nestler Eric J. 
Published: March 2019

AbstractThe transcription factor ΔFosB has been proposed as a molecular switch for the transition from casual, volitional drug use into a chronically addicted state, but the upstream regulatory mechanisms governing ΔFosB expression are incompletely understood. In this study, we find a novel regulatory role for the transcription factor E2F3, recently implicated in transcriptional regulation by cocaine, in controlling ΔFosB induction in the mouse nucleus accumbens (NAc) following cocaine administration. We find that an E2F consensus sequence 500 bp upstream of theFosbtranscription start site is enriched for E2F3 specifically over other E2F isoforms. We further conclude that ΔFosB expression is regulated specifically by E2F3a, not E2F3b, thatE2f3aexpression is specific to D1 receptor-expressing medium spiny neurons, and that E2F3a overexpression in NAc recapitulates the induction ofFosbandΔFosbmRNA expression observed after chronic cocaine exposure. E2F3a knockdown in NAc does not abolishΔFosbinduction by cocaine, a result consistent with previously published data showing that singular knockdown of upstream regulators of ΔFosB is insufficient to block cocaine-induced expression. Finally, to elucidate potential combinatorial epigenetic mechanisms involved in E2F3a’s regulation ofFosb, we explore H3K4me3 enrichment at theFosbpromoter and find that it is not enhanced by E2F3a overexpression, suggesting that it may instead be a pre-existing permissive mark allowing for E2F3a to interact withFosb. Together, these findings support a role for E2F3a as a novel, upstream regulator of the addiction-mediating transcription factor ΔFosB in NAc.
Synaptic Basis for Contrast-Dependent Shifts in Functional Identity in Mouse V1
Authors: Yunzab Molis , Choi Veronica , Meffin Hamish , Cloherty Shaun L. , Priebe Nicholas J. , Ibbotson Michael R. 
Published: March 2019

AbstractA central transformation that occurs within mammalian visual cortex is the change from linear, polarity-sensitive responses to nonlinear, polarity-insensitive responses. These neurons are classically labelled as either simple or complex, respectively, on the basis of their response linearity (Skottun et al., 1991). While the difference between cell classes is clear when the stimulus strength is high, reducing stimulus strength diminishes the differences between the cell types and causes some complex cells to respond as simple cells (Crowder et al., 2007; van Kleef et al., 2010; Hietanen et al., 2013). To understand the synaptic basis for this shift in behavior, we usedin vivowhole-cell recordings while systematically shifting stimulus contrast. We find systematic shifts in the degree of complex cell responses in mouse primary visual cortex (V1) at the subthreshold level, demonstrating that synaptic inputs change in concert with the shifts in response linearity and that the change in response linearity is not simply due to the threshold nonlinearity. These shifts are consistent with a visual cortex model in which the recurrent amplification acts as a critical component in the generation of complex cell responses (Chance et al., 1999).
Effects of dietary fat on insulin secretion in subjects with the metabolic syndrome
Authors: Gulseth Hanne L , Gjelstad Ingrid M F , Tiereny Audrey C , McCarthy Danielle , Lovegrove Julie A , Defoort Catherine , Blaak Ellen E , Lopez-Miranda Jose , Dembinska-Kiec Aldona , Risérus Ulf , Roche Helen M , Drevon Christian A , Birkeland Kåre I 
Published: March 2019

Objective
Impaired insulin secretion and action contribute to the development of type 2 diabetes. Dietary fat modification may improve insulin sensitivity, whereas the effect on insulin secretion is unclear. We investigated the effect of dietary fat modification on insulin secretion in subjects with the metabolic syndrome.

Design
In a 12-week pan-European parallel, randomized controlled dietary intervention trial (LIPGENE), 486 subjects were assigned to four isoenergetic diets: high-fat diets rich in saturated fat (HSFA) or monounsaturated fat (HMUFA) or low-fat, high-complex carbohydrate diets with (LFHCC n-3) or without (LFHCC control) 1.2 g/day of n-3 PUFA supplementation. Insulin secretion was estimated as acute insulin response to glucose (AIRg) and disposition index (DI), modeled from an intravenous glucose tolerance test.

Results
There were no overall effect of the dietary intervention on AIRg and DI in the total cohort, in neither the high-fat nor LFHCC groups. We observed significant diet*fasting glucose category interactions for AIRg (P = 0.021) and DI (P = 0.001) in the high-fat groups. In subjects with normal fasting glucose and preserved first phase insulin secretion, the HMUFA diet increased, whereas the HSFA diet reduced AIRg (P = 0.015) and DI (P = 0.010).

Conclusions
The effects of dietary fat modification on insulin secretion were minor, and only evident in normoglycemic subjects. In this case, the HMUFA diet improved AIRg and DI, as compared to the HSFA diet.
Pituitary deficiency and precocious puberty after childhood severe traumatic brain injury: a long-term follow-up prospective study
Authors: Dassa Yamina , Crosnier Hélène , Chevignard Mathilde , Viaud Magali , Personnier Claire , Flechtner Isabelle , Meyer Philippe , Puget Stéphanie , Boddaert Nathalie , Breton Sylvain , Polak Michel 
Published: March 2019

ObjectivesChildhood traumatic brain injury (TBI) is a public health issue. Our objectives were to determine the prevalence of permanent pituitary hormone deficiency and to detect the emergence of other pituitary dysfunctions or central precocious puberty several years after severe TBI.DesignFollow-up at least 5 years post severe TBI of a prospective longitudinal study.PatientsOverall, 66/87 children, who had endocrine evaluation 1 year post severe TBI, were included (24 with pituitary dysfunction 1 year post TBI).Main outcome measuresIn all children, the pituitary hormones basal levels were assessed at least 5 years post TBI. Growth hormone (GH) stimulation tests were performed 3–4 years post TBI in children with GH deficiency (GHD) 1 year post TBI and in all children with low height velocity (<−1 DS) or low IGF-1 (<−2 DS). Central precocious puberty (CPP) was confirmed by GnRH stimulation test.ResultsOverall, 61/66 children were followed up 7 (5–10) years post TBI (median; (range)); 17/61 children had GHD 1 year post TBI, and GHD was confirmed in 5/17 patients. For one boy, with normal pituitary function 1 year post TBI, GHD was diagnosed 6.5 years post TBI. 4/61 patients developed CPP, 5.7 (2.4–6.1) years post-TBI. Having a pituitary dysfunction 1 year post TBI was significantly associated with pituitary dysfunction or CPP more than 5 years post TBI.ConclusionSevere TBI in childhood can lead to permanent pituitary dysfunction; GHD and CPP may appear after many years. We recommend systematic hormonal assessment in children 1 year after severe TBI and a prolonged monitoring of growth and pubertal maturation. Recommendations should be elaborated for the families and treating physicians.