bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laboratory practices for identification and reporting of carbapenem-resistant Enterobacteriaceae in Department of Veterans Affairs facilities
Authors: Fitzpatrick Margaret A. , Suda Katie J. , Ramanathan Swetha , Guihan Marylou , Brown Charnetta , Safdar Nasia , Evans Martin , Jones Makoto M. , Pfeiffer Christopher D. , Klutts J. Stacey , Icardi Michael , Perencevich Eli , Rubin Michael , Evans Charlesnika T. ,   the QUERI CARRIAGE Program
Published: March 2019

AbstractLaboratory identification of carbapenem-resistant Enterobacteriaceae (CRE) is a key step in controlling its spread. Our survey showed that most Veterans Affairs laboratories follow VA guidelines for initial CRE identification, whereas 55.0% use PCR to confirm carbapenemase production. Most respondents were knowledgeable about CRE guidelines. Barriers included staffing, training, and financial resources.
China's Conflict Mediation and the Durability of the Principle of Non-Interference: The Case of Post-2014 Afghanistan
Authors: Hirono Miwa 
Published: March 2019

AbstractChina's efforts in conflict mediation are an important test of the durability of the principle of non-interference. By analysing the approaches and means of China's post-2014 mediation efforts in Afghanistan, this article finds that China's behaviour shows it engages in medium-level interference in domestic affairs, but mostly with the host government's concurrence. This is because of the two forms China's mediation takes. In a bilateral context, China's mediation takes the form of “incentivizing mediation,” in which its economic power, and its omnidirectional foreign policy, provide incentives or leverage for warring factions to come to the negotiation table, but which also lets the warring factions formulate their own roadmap to peace talks. In a multilateral context, China sometimes engages in “formulative mediation,” in which the mediators, not the disputing parties, formulate a roadmap to peace talks.
Integration of a recent infection testing algorithm into HIV surveillance in Ireland: improving HIV knowledge to target prevention
Authors: Robinson E. , Moran J. , O'Donnell K. , Hassan J. , Tuite H. , Ennis O. , Cooney F. , Nugent E. , Preston L. , O'Dea S. , Doyle S. , Keating S. , Connell J. , De Gascun C. , Igoe D. 
Published: March 2019

AbstractRecent infection testing algorithms (RITA) for HIV combine serological assays with epidemiological data to determine likely recent infections, indicators of ongoing transmission. In 2016, we integrated RITA into national HIV surveillance in Ireland to better inform HIV prevention interventions. We determined the avidity index (AI) of new HIV diagnoses and linked the results with data captured in the national infectious disease reporting system. RITA classified a diagnosis as recent based on an AI < 1.5, unless epidemiological criteria (CD4 count <200 cells/mm3; viral load <400 copies/ml; the presence of AIDS-defining illness; prior antiretroviral therapy use) indicated a potential false-recent result. Of 508 diagnoses in 2016, we linked 448 (88.1%) to an avidity test result. RITA classified 12.5% of diagnoses as recent, with the highest proportion (26.3%) amongst people who inject drugs. On multivariable logistic regression recent infection was more likely with a concurrent sexually transmitted infection (aOR 2.59; 95% CI 1.04–6.45). Data were incomplete for at least one RITA criterion in 48% of cases. The study demonstrated the feasibility of integrating RITA into routine surveillance and showed some ongoing HIV transmission. To improve the interpretation of RITA, further efforts are required to improve completeness of the required epidemiological data.
Online symptom checker diagnostic and triage accuracy for HIV and hepatitis C
Authors: Berry A.C. , Cash B.D. , Wang B. , Mulekar M.S. , Van Haneghan A.B. , Yuquimpo K. , Swaney A. , Marshall M. C. , Green W.K. 
Published: March 2019

AbstractWe sought to address the prior limitations of symptom checker accuracy by analysing the diagnostic and triage feasibility of online symptom checkers using a consecutive series of real-life emergency department (ED) patient encounters, and addressing a complex patient population – those with hepatitis C or HIV. We aimed to study the diagnostic and triage accuracy of these symptom checkers in relation to an emergency room physician-determined diagnosis. An ED retrospective analysis was performed on 8363 consecutive adult patients. Eligible patients included: 90 HIV, 67 hepatitis C, 11 both HIV and hepatitis C. Five online symptom checkers were utilised for diagnosis (Mayo Clinic, WebMD, Symptomate, Symcat, Isabel), three with triage capabilities. Symptom checker output was compared with ED physician-determined diagnosis data in regards to diagnostic accuracy and differential diagnosis listing, along with triage advice. All symptom checkers, whether for combined HIV and hepatitis C, HIV alone or hepatitis C alone had poor diagnostic accuracy in regards to Top1 (<20%), Top3 (<35%), Top10 (<40%), Listed at All (<45%). Significant variations existed for each individual symptom checker, as some appeared more accurate for listing the diagnosis in the top of the differential, vs. others more apt to list the diagnosis at all. In regards to ED triage data, a significantly higher percentage of hepatitis C patients (59.7%; 40/67) were found to have an initial diagnosis with emergent criteria than HIV patients (35.6%; 32/90). Symptom checker diagnostic capabilities are quite inferior to physician diagnostic capabilities. Complex patients such as those with HIV or hepatitis C may carry a more specific differential diagnosis, warranting symptom checkers to have diagnostic algorithms accounting for such complexity. Symptom checkers carry the potential for real-time epidemiologic monitoring of patient symptoms, as symptom entries and subsequent symptom checker diagnosis could allow health officials a means to track illnesses in specific patient populations and geographic regions. In order to do this, accurate and reliable symptom checkers are warranted.
Towns, government, legislation and the ‘police’ in Jamaica and the British Atlantic, 1770–1805
Authors: Graham Aaron 
Published: March 2019

AbstractUrban renewal in the British Isles in the long eighteenth century was based on new municipal powers made possible by parliament. Focusing on Jamaica between 1770 and 1805, which passed legislation for the ‘policing’ – in the broader Scottish sense – of its towns, demonstrates that it was a global phenomenon common to the whole British Atlantic. However, the solutions it produced were also specific to local circumstances. Jamaican elites feared invasion, revolt and the dissolution of the slave society. Their police acts reflected these concerns, and demonstrate the alternative pathway that urban modernity took in this part of the British Atlantic.
Electron Beam Effects on Oxide Thin Films—Structure and Electrical Property Correlations
Authors: Neelisetty Krishna Kanth , Mu Xiaoke , Gutsch Sebastian , Vahl Alexander , Molinari Alan , von Seggern Falk , Hansen Mirko , Scherer Torsten , Zacharias Margit , Kienle Lorenz , Chakravadhanula VS Kiran , Kübel Christian 
Published: March 2019

AbstractIn situtransmission electron microscope (TEM) characterization techniques provide valuable information on structure–property correlations to understand the behavior of materials at the nanoscale. However, understanding nanoscale structures and their interaction with the electron beam is pivotal for the reliable interpretation ofin situ/ex situTEM studies. Here, we report that oxides commonly used in nanoelectronic applications, such as transistor gate oxides or memristive devices, are prone to electron beam induced damage that causes small structural changes even under very low dose conditions, eventually changing their electrical properties as examined viain situmeasurements. In this work, silicon, titanium, and niobium oxide thin films are used forin situTEM electrical characterization studies. The electron beam induced reduction of the oxides turns these insulators into conductors. The conductivity change is reversible by exposure to air, supporting the idea of electron beam reduction of oxides as primary damage mechanism. Through these measurements we propose a limit for the critical dose to be considered forin situscanning electron microscopy and TEM characterization studies.
Failure Evaluation of a SiC/SiC Ceramic Matrix Composite During In-Situ Loading Using Micro X-ray Computed Tomography
Authors: Thornton John , Arhatari Benedicta D. , Sesso Mitchell , Wood Chris , Zonneveldt Matthew , Kim Sun Yung , Kimpton Justin A. , Hall Chris 
Published: March 2019

AbstractIn this study, we have examined ceramic matrix composites with silicon carbide fibers in a melt-infiltrated silicon carbide matrix (SiC/SiC). We subjected samples to tensile loads while collecting micro X-ray computed tomography images. The results showed the expected crack slowing mechanisms and lower resistance to crack propagation where the fibers ran parallel and perpendicular to the applied load respectively. Cracking was shown to initiate not only from the surface but also from silicon inclusions. Post heat-treated samples showed longer fiber pull-out than the pristine samples, which was incompatible with previously proposed mechanisms. Evidence for oxidation was identified and new mechanisms based on oxidation or an oxidation assisted boron nitride phase transformation was therefore proposed to explain the long pull-out. The role of oxidation emphasizes the necessity of applying oxidation resistant coatings on SiC/SiC.
Estimating the Frequency of Sudden Stratospheric Warming Events From Surface Observations of the North Atlantic Oscillation
Authors: Domeisen Daniela I.V. 
Published: March 2019

AbstractSudden stratospheric warming (SSW) events can exhibit long‐lasting surface impacts that promise improvements in medium‐range to seasonal predictability. Their surface impact is dominated by the negative phase of the North Atlantic Oscillation (NAO). Hence, the question arises if stratospheric variability, and in particular the frequency of SSW events, can in turn be estimated from surface NAO conditions. This is especially relevant for the period before frequent upper air observations became available, while daily surface observations of the NAO date back to 1850. The surface impact is here quantified by NAO characteristics that are commonly observed after SSW events: a switch from a positive to a negative NAO and an extended persistence of the negative NAO, termed NAO events. Two thirds of SSW events are found to be followed by either a persistence or switch NAO event, and a quarter of SSW events are followed by both. On the other hand, less than 25% of winter surface NAO events are preceded by a SSW event. Based on these findings, an index purely based on surface NAO observations is derived that estimates SSW frequency for the satellite era and extends it back to 1850, indicating that decadal stratospheric variability was present for the entire time series, with no significant trend. The minimum in SSW frequency in the 1990s is found to be coincident with the longest absence of NAO events since 1850, indicating that the early 1990s may constitute the longest absence of SSW events for the 150‐year record.
Investigating Transport and Dissipation in the Subauroral E Region With Ionospheric Modification Experiments and Very High Frequency Radar Backscatter
Authors: Hysell D. L. , Munk J. , McCarrick M. 
Published: March 2019

AbstractIonospheric modification experiments have been performed at the High‐Frequency Active Auroral Research Program (HAARP) facility in Gakona, Alaska, using a Very High Frequency (VHF) coherent scatter radar in Homer, Alaska, for experimental diagnostics. The experiments were intended to determine the threshold pump electric field required to initiate thermal parametric instability in the E region. The pump power level was ramped systematically to determine the threshold, and the experiment was repeated at four closely spaced pump frequencies. This provided threshold estimates at four E region altitudes. The theory for thermal parametric instability based on the work of Dysthe et al. (1983, https://doi.org/10.1063/1.863993) has been modified for application in the E region. The theory considers magneto‐ionic effects on the pump mode, linear mode conversion theory for upper hybrid wave generation, wave heating, and the effects of transport and dissipation based on fluid theory. The theory amounts to an eigenvalue problem where the eigenvalue is the threshold pump electric field for instability. The theory shows how the threshold depends on ionospheric transport coefficients and on the fractional cooling rate for inelastic electron‐neutral collisions. The theoretical predictions for threshold are roughly consistent with experimental values although the latter are probably affected by excess ionospheric absorption.
Unraveling the biophysical underpinnings to the success of multispecies biofilms in porous environments
Authors: Scheidweiler David , Peter Hannes , Pramateftaki Paraskevi , de Anna Pietro , Battin Tom J 
Published: March 2019

Abstract
Biofilms regulate critical processes in porous ecosystems. However, the biophysical underpinnings of the ecological success of these biofilms are poorly understood. Combining experiments with fluidic devices, sequencing and modeling, we reveal that architectural plasticity enhances space exploitation by multispecies biofilms in porous environments. Biofilms consistently differentiated into an annular base biofilm coating the grains and into streamers protruding from the grains into the pore space. Although different flow-related processes governed the differentiation of these architectures, both BB and streamers were composed of similar bacterial assemblages. This is evidence for architectural plasticity. Architectural plasticity allowed for complementary use of the space provided by the grain–pore complexes, which increased biofilm carrying capacity at the larger scale of the porous system. This increase comes potentially at the cost of a tradeoff. Contrasting time scales of oxygen replenishment and consumption, we show that streamers locally inhibit the growth of the BB downstream from the grains. Our study provides first insights into the biophysical underpinnings to the success of multispecies biofilms in porous environments.
Inhibition of lymphatic proliferation by the selective VEGFR-3 inhibitor SAR131675 ameliorates diabetic nephropathy in db/db mice
Authors: Hwang Seun Deuk , Song Joon Ho , Kim Yaeni , Lim Ji Hee , Kim Min Young , Kim Eun Nim , Hong Yu Ah , Chung Sungjin , Choi Bum Soon , Kim Yong-Soo , Park Cheol Whee 
Published: March 2019

AbstractRecent studies have demonstrated that chronic inflammation-induced lymphangiogenesis plays a crucial role in the progression of various renal diseases, including diabetic nephropathy. SAR131675 is a selective vascular endothelial cell growth factor receptor-3 (VEGFR-3)-tyrosine kinase inhibitor that acts as a ligand for VEGF-C and VEGF-D to inhibit lymphangiogenesis. In this study, we evaluated the effect of SAR131675 on renal lymphangiogenesis in a mouse model of type 2 diabetes. Male C57BLKS/J db/m and db/db mice were fed either a regular chow diet or a diet containing SAR131675 for 12 weeks from 8 weeks of age. In addition, we studied palmitate-induced lymphangiogenesis in human kidney-2 (HK2) cells and RAW264.7 monocytes/macrophages, which play a major role in lymphangiogenesis in the kidneys. SAR131475 ameliorated dyslipidemia, albuminuria, and lipid accumulation in the kidneys of db/db mice, with no significant changes in glucose and creatinine levels and body weight. Diabetes-induced systemic inflammation as evidenced by increased systemic monocyte chemoattractant protein-1 and tumor necrosis factor-α level was decreased by SAR131475. SAR131475 ameliorated the accumulation of triglycerides and free fatty acids and reduced inflammation in relation to decreased chemokine expression and pro-inflammatory M1 macrophage infiltration in the kidneys. Downregulation of VEGF-C and VEGFR-3 by SAR131475 inhibited lymphatic growth as demonstrated by decreased expression of LYVE-1 and podoplanin that was further accompanied by reduced tubulointerstitial fibrosis, and inflammation in relation to improvement in oxidative stress and apoptosis. Treatment with SAR131475 improved palmitate-induced increase in the expression of VEGF-C, VEGFR-3, and LYVE-1, along with improvement in cytosolic and mitochondrial oxidative stress in RAW264.7 and HK2 cells. Moreover, the enhanced expression of M1 phenotypes in RAW264.7 cells under palmitate stress was reduced by SAR131475 treatment. The results suggest that modulation of lymphatic proliferation in the kidneys is a new treatment approach for type 2 diabetic nephropathy and that SAR131675 is a promising therapy to ameliorate renal damage by reducing lipotoxicity-induced lymphangiogenesis.
TNFRSF receptor-specific antibody fusion proteins with targeting controlled FcγR-independent agonistic activity
Authors: Medler Juliane , Nelke Johannes , Weisenberger Daniela , Steinfatt Tim , Rothaug Moritz , Berr Susanne , Hünig Thomas , Beilhack Andreas , Wajant Harald 
Published: March 2019

AbstractAntibodies specific for TNFRSF receptors that bind soluble ligands without getting properly activated generally act as strong agonists upon FcγR binding. Systematic analyses revealed that the FcγR dependency of such antibodies to act as potent agonists is largely independent from isotype, FcγR type, and of the epitope recognized. This suggests that the sole cellular attachment, achieved by Fc domain-FcγR interaction, dominantly determines the agonistic activity of antibodies recognizing TNFRSF receptors poorly responsive to soluble ligands. In accordance with this hypothesis, we demonstrated that antibody fusion proteins harboring domains allowing FcγR-independent cell surface anchoring also act as strong agonist provided they have access to their target. This finding defines a general possibility to generate anti-TNFRSF receptor antibodies with FcγR-independent agonism. Moreover, anti-TNFRSF receptor antibody fusion proteins with an anchoring domain promise superior applicability to conventional systemically active agonists when an anchoring target with localized disease associated expression can be addressed.
Neuronal AMP-activated protein kinase hyper-activation induces synaptic loss by an autophagy-mediated process
Authors: Domise Manon , Sauvé Florent , Didier Sébastien , Caillerez Raphaëlle , Bégard Séverine , Carrier Sébastien , Colin Morvane , Marinangeli Claudia , Buée Luc , Vingtdeux Valérie 
Published: March 2019

AbstractAlzheimer’s disease (AD) is a neurodegenerative disorder characterized by synaptic loss that leads to the development of cognitive deficits. Synapses are neuronal structures that play a crucial role in memory formation and are known to consume most of the energy used in the brain. Interestingly, AMP-activated protein kinase (AMPK), the main intracellular energy sensor, is hyper-activated in degenerating neurons in several neurodegenerative diseases, including AD. In this context, we asked whether AMPK hyper-activation could influence synapses' integrity and function. AMPK hyper-activation in differentiated primary neurons led to a time-dependent decrease in pre- and post-synaptic markers, which was accompanied by a reduction in synapses number and a loss of neuronal networks functionality. The loss of post-synaptic proteins was mediated by an AMPK-regulated autophagy-dependent pathway. Finally, this process was also observed in vivo, where AMPK hyper-activation primed synaptic loss. Overall, our data demonstrate that during energetic stress condition, AMPK might play a fundamental role in the maintenance of synaptic integrity, at least in part through the regulation of autophagy. Thus, AMPK might represent a potential link between energetic failure and synaptic integrity in neurodegenerative conditions such as AD.
Circadian control of stress granules by oscillating EIF2α
Authors: Wang Ruiqi , Jiang Xin , Bao Puhua , Qin Meiling , Xu Jin 
Published: March 2019

AbstractStress granule formation is important for stress response in normal cells and could lead to chemotherapy resistance in cancer cells. Aberrant stress granule dynamics are also known to disrupt proteostasis, affect RNA metabolism, and contribute to neuronal cell death. Meanwhile, circadian abnormality is an aging-related risk factor for cancer and neurodegeneration. Whether stress granule dynamics are circadian regulated is entirely unknown. Here we show that the formation of stress granules varied by zeitgeber time in mouse liver. Moreover, altering circadian regulation by silencing the core circadian gene Bmal1 in a cell line expressing an endogenous GFP-tagged G3BP1 significantly increased stress granule dynamics, while the overexpression of Bmal1 decreased them. Surprisingly, increased stress granule dynamics and formation by transient decrease of BMAL1 coincided with increased resistance to stress-induced cell death. The circadian regulation of stress granules was mediated by oscillating eIF2α expression. At zeitgeber time when BMAL1 and eIF2α were at nadir, reduction of unphosphorylated eIF2α could significantly alter the ratio of phosphorylated/total eIF2α and quickly lead to increased formation of stress granules. Therefore, diurnal oscillating eIF2α connects the circadian cue to a cellular stress response mechanism that is vital for both neurodegeneration and cancer.
Sustained NF-κB-STAT3 signaling promotes resistance to Smac mimetics in Glioma stem-like cells but creates a vulnerability to EZH2 inhibition
Authors: da Hora Cintia Carla , Pinkham Kelsey , Carvalho Litia , Zinter Max , Tabet Elie , Nakano Ichiro , Tannous Bakhos A. , Badr Christian E. 
Published: March 2019

AbstractGlioblastoma is an incurable and highly aggressive brain tumor. Understanding therapeutic resistance and survival mechanisms driving this tumor type is key to finding effective therapies. Smac mimetics (SM) emerged as attractive cancer therapeutics particularly for tumor populations that are highly resistant to conventional apoptosis-inducing therapies. We evaluated the therapeutic efficacy of SM on Glioma stem-like cells (GSCs) and showed that this family of compounds stimulates an adaptive response triggered by TNFα. Increased expression of TNFα results in a prolonged and sustained activation of NF-κB and STAT3 signaling thus activating several tumor cell resistance mechanisms in GSCs. We show that STAT3 activation is contingent on EZH2 activation and uncover a synergistic lethality between SM and EZH2 inhibitors. Therapeutic inhibition of EZH2 impaired the viability of SM-treated GSCs. Our study outlines the molecular underpinnings of SM resistance in glioblastoma and provides mechanistic insight to overcome this resistance and increase therapeutic efficacy.