bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns of genetic diversity in African forest elephants living in a human‐modified landscape in southwest Gabon
Authors: Johnson Mireille B. , Parker Lillian D. , Vanthomme Hadrien , Tchignoumba Landry , Deichmann Jessica L. , Maldonado Jesús E. , Korte Lisa , Alonso Alfonso 
Published: September 2019

AbstractWe investigated the patterns of genetic diversity and structure of African forest elephants in a human‐modified landscape in the Gamba complex of protected areas (GCPA), a tropical wilderness area along the southwest coast of Gabon. We collected 298 elephant fecal samples from four sites (Sette Cama, Gamba area, Vera Plains, and Mayonami), along approximately 80 km of coastline from north to south. We used microsatellites and mitochondrial DNA (mtDNA) to successfully genotype 295 of the 298 fecal samples, and identified 213 individuals. Using sex markers, we identified 84 males and 118 females; we could not determine the sex of the remaining 11 individuals. We also characterized the sex, group size, and social status of crop‐raiding elephants and did not find characteristics distinguishing them from nonraiders. Overall, our mtDNA and microsatellite markers revealed that elephants in the research area maintain high levels of genetic variation and low levels of subdivision. Gene flow appears to be mostly mediated by male dispersal away from natal herds. Our structure analysis revealed two highly admixed genetic clusters, attributable to high connectivity among the protected areas. However, forest areas within the GCPA have become increasingly fragmented by human‐induced habitat modification. We detected a pattern of isolation by distance, accentuated by the presence of the town of Gamba between Sette‐Cama and Mayonami. We found a high degree of connectivity among sampling locations within the GCPA. This supports the importance of establishing agricultural best practices to reduce habitat loss that may sever gene exchange and to maintain connectivity, as well as to avoid human–elephant conflict that can result in retaliatory killing of elephants in this area. This study emphasizes the importance of conducting baseline monitoring of demographic data, genetic diversity, and structure to enable future comparisons to assess the long‐term impact of human‐induced habitat fragmentation.
Risk of potential pesticide use to honeybee and bumblebee survival and distribution: A country‐wide analysis for The Netherlands
Authors: Yasrebi‐de Kom Izak A. R. , Biesmeijer Jacobus C. , Aguirre‐Gutiérrez Jesús 
Published: September 2019

AbstractAimBees play an important role in natural ecosystems and the world's food supply. In the past decades, bee abundance and diversity have declined globally. This has resulted in decreased pollination services for natural ecosystems and the agricultural sector at the field scale. One of the causes of the decline in bee abundance and diversity is the use of pesticides. Linking pesticide use, land use and bee presence could provide crucial insights into areas, and pesticides that pose a significant threat to the abundance and diversity of bees. Obtaining actual figures of farmer pesticide use is rarely possible. Therefore, we designed a method to study the effects of potential pesticide use on the survival and distribution of honeybees and bumblebees.LocationThe Netherlands.MethodsA pesticide risk model was implemented incorporating a hazard quotient as the risk assessment. The number of allowed pesticide active ingredients per crop that could pose a risk to honeybees and bumblebees was linked to the Dutch crop parcel locations for 2015 and 2016. The potential pesticide risk maps were analysed using honeybee colony survival and bumblebee presence data.ResultsNon‐significant negative effects of potential pesticide risk on honeybee colony survival and bumblebee presence were found. A significant negative effect of greenhouses was identified for both honeybees and bumblebees. The most important factors in the models predicting honeybee colony survival and bumblebee presence were urban land areas and natural grasslands, respectively, both showing a positive effect.Main conclusionsHere, the first attempt to estimate and map pesticide risk to bees in the Netherlands in a country‐wide manner is presented. The results could provide crucial high‐resolution insights for bee conservation action and facilitate the increase of pollination services in natural ecosystems and the agricultural sector on a local and country‐wide scale.
Caveat consumptor notitia museo: Let the museum data user beware
Authors: Nekola Jeffrey C. , Hutchins Benjamin T. , Schofield Alison , Najev Briante , Perez Kathryn E. 
Published: September 2019

AbstractIssueLot accession information from natural history collections represents a potentially vital source of large datasets to test biodiversity, biogeography and macroecology hypotheses. But does such information provide an accurate portrayal of the natural world? We review the many types of bias and error intrinsic to museum collection data and consider how these factors may affect their ability to accurately test ecological hypotheses.EvidenceWe considered all Texas land snail collections from the two major repositories in the state and compared them with an ecological sample drawn across the same landscape. We found that museum collection localities were biased in favour of regions with higher human population densities and iconic destinations. They also tended to be made during attractive temporal windows. Small, uncharismatic taxa tended to be under‐collected while larger, charismatic species were over‐collected. As a result, for most species it was impossible to use museum lot frequency to accurately predict frequency and abundance in an ecological sample. Species misidentification rate was approximately 20%, while 4% of lots represented more than one species. Errors were spread across the entire shell size spectrum and were present in 75% of taxonomic families. Contingency table analysis documented significant dependence of both misidentification and mixed lot rates upon shell size and family richness.ConclusionResearchers should limit their use of museum record data to situations where their inherent biases and errors are irrelevant, rectifiable or explicitly considered. At the same time museums should begin incorporating expert specimen verification into their digitization programs.
Environmental factors explain the spatial mismatches between species richness and phylogenetic diversity of terrestrial mammals
Authors: Barreto Elisa , Graham Catherine H. , Rangel Thiago F. 
Published: September 2019

AbstractAimExplore the spatial variation of the relationships between species richness (SR), phylogenetic diversity (PD) and environmental factors to infer the possible mechanisms underlying patterns of diversity in different regions of the globe.LocationGlobal.Time periodPresent day.Major taxa studiedTerrestrial mammals.MethodsWe used a hexagonal grid to map SR and PD of mammals and four environmental factors (temperature, productivity, elevation and climate‐change velocity since the Last Glacial Maximum). We related those variables through direct and indirect pathways using a novel combination of path analysis and geographically weighted regression to account for spatial non‐stationarity of path coefficients.ResultsSpecies richness, PD and environmental factors relate differently across the geographical space, with most relationships varying in both magnitude and direction. Species richness is associated with lower PD in much of the tropics and in the Americas, which reflects the tropical origin and the recent diversification of some mammalian clades in these regions. Environmental effects on PD are predominantly mediated by their effects on SR. But once richness is controlled for, the relationships between environmental factors and PD (i.e. PDSR) highlight environmentally driven changes in species composition. Environmental–PDSR relationships suggest that the relative importance of different mechanisms driving biodiversity shifts spatially. Across most of the globe, temperature and productivity are the strongest predictors of richness, whereas PDSR is best predicted by temperature.Main conclusionsRichness explains most spatial variation in PD, but both dimensions of biodiversity respond differently to environmental conditions across the globe, as indicated by the spatial mismatches in the relationships between environmental factors and these two types of diversity. We show that accounting for spatial non‐stationarity and environmental effects on PD while controlling for richness uncovers a more complex scenario of drivers of biodiversity than previously observed.
Blood donor–recipient sex mismatch: outcomes impacted?
Authors: Tran Minh‐Ha 
Published: September 2019

Background and objectivesA number of recent publications have questioned whether female donor transfusions (particularly of red cells) or donor–recipient sex mismatch are associated with increased risk for mortality. A systematic review was conducted to further explore this concern.Materials and methodsA literature search was performed to identify original studies linking red‐blood‐cell transfusion donor or recipient gender to mortality risk. Exclusion criteria included (1) absence of a focus on gender mismatch and mortality outcomes, (2) nonclinical studies, (3) non‐English language studies.ResultsThe initial search strategy yielded 136 results and 8 studies met all selection criteria. An additional 2 studies were found by supplemental search. Six of the ten studies reported increased mortality risk with sex mismatch, while four reported no increase in mortality. Study end‐points varied, thus limiting generalizability. Conflicting evidence was noted in regard to female donor sex, sex mismatch, directionality of risk, age of donors and recipients, and transfusion burden.ConclusionAdditional studies are needed to resolve this issue. As the majority of studies relied upon a ‘big data’ approach, there is concern for subtle confounding.
High-Sensitivity Troponin and the Application of Risk Stratification Thresholds in Patients With Suspected Acute Coronary Syndrome
Authors: Bularga Anda , Lee Kuan Ken , Stewart Stacey , Ferry Amy V. , Chapman Andrew R. , Marshall Lucy , Strachan Fiona E. , Cruickshank Anne , Maguire Donogh , Berry Colin , Findlay Iain , Shah Anoop S.V. , Newby David E. , Mills Nicholas L. , Anand Atul ,   On behalf of the High-STEACS Investigators
Published: September 2019

Background:
Guidelines acknowledge the emerging role of high-sensitivity cardiac troponin (hs-cTnl) for risk stratification and the early rule-out of myocardial infarction, but multiple thresholds have been described. We evaluate the safety and effectiveness of risk stratification thresholds in patients with suspected acute coronary syndrome.

Methods:
Consecutive patients with suspected acute coronary syndrome (n=48 282) were enrolled in a multicenter trial across 10 hospitals in Scotland. In a prespecified secondary and observational analysis, we compared the performance of the limit of detection (<2 ng/L) and an optimized risk stratification threshold (<5 ng/L) using the Abbott high-sensitivity troponin I assay. Patients with myocardial injury at presentation, with ≤2 hours of symptoms or with ST-segment elevation myocardial infarction were excluded. The negative predictive value was determined in all patients and in subgroups for a primary outcome of myocardial infarction or cardiac death within 30 days. The secondary outcome was myocardial infarction or cardiac death at 12 months, with risk modeled using logistic regression adjusted for age and sex.

Results:
In total, 32 837 consecutive patients (61±17 years, 47% female) were included, of whom 23 260 (71%) and 12,716 (39%) had hs-cTnl concentrations of <5 ng/L and <2 ng/L at presentation. The negative predictive value for the primary outcome was 99.8% (95% CI, 99.7%–99.8%) and 99.9% (95% CI, 99.8%–99.9%) in those with hs-cTnl concentrations of <5 ng/L and <2 ng/L, respectively. At both thresholds, the negative predictive value was consistent in men and women and across all age groups, although the proportion of patients identified as low risk fell with increasing age. Compared with patients with hs-cTnl concentrations of ≥5 ng/L but <99th centile, the risk of myocardial infarction or cardiac death at 12 months was 77% lower in those <5 ng/L (5.3% vs 0.7%; adjusted odds ratio, 0.23 [95% CI, 0.19–0.28]) and 80% lower in those <2 ng/L (5.3% vs 0.3%; adjusted odds ratio, 0.20 [95% CI, 0.14–0.29]).

Conclusions:
Use of risk stratification thresholds for hs-cTnl identify patients with suspected acute coronary syndrome and at least 2 hours of symptoms as low risk at presentation irrespective of age and sex.

Clinical Trial Registration:

URL:
https://www.clinicaltrials.gov
. Unique identifier: NCT01852123.
Improving the quality of life of people with advanced respiratory disease and severe breathlessness
Authors: Booth Sara , Johnson Miriam J. 
Published: September 2019

Advanced respiratory disease imposes a greater symptom burden than many cancers but not does have comparable recognition of the need for supportive and palliative care or the infrastructure for its systematic delivery. Consequently, many people with advanced respiratory disease (and those closest to them) have a poor quality of life, disabled by chronic breathlessness, fatigue and other symptoms. They are socially isolated by the consequences of long-term illness and are often financially impoverished. The past decade has seen an increasing realisation that care for this group must improve and that symptom management must be prioritised. Clinical guidelines recommend person-centred care, including access to supportive and palliative care as needed, as part of standard medical practice. Advanced lung disease clinics and specialist breathlessness services (pioneered within palliative care) are developing within respiratory medicine services but are provided inconsistently.This review covers the comprehensive assessment of the patient with advanced respiratory disease, the importance of supporting carers and the current best practice in the management of breathlessness, fatigue and cough. It also suggests ways to incorporate person-centred care into the general respiratory clinic, assisted by better liaison with specialist palliative and primary care. Emerging evidence shows that excellent symptom management leads to better clinical outcomes and reduces inappropriate use of emergency medical services.Key pointsPeople living with advanced respiratory disease and severe chronic breathlessness (and those closest to them) have a poor quality of life.Chronic breathlessness is a disabling symptom, and acute-on-chronic/episodic breathlessness is frightening to experience and observe.Chronic breathlessness imposes profound physical limitations and psychosocial burdens on those suffering from it or living with someone experiencing it.Fatigue and cough are two other cardinal symptoms of advanced respiratory disease, with very detrimental effects on quality of life.The impact of all these symptoms can be alleviated to a variable extent by a predominantly non-drug complex intervention.Many of the interventions are delivered primarily by allied health or nursing professionals.Doctors, nurses and other health professionals also need to play an active part in promoting quality of life as part of excellent medical care.A person-centred, psychologically informed approach is needed by all clinicians treating patients with advanced respiratory disease.Educational aimsTo give specialist respiratory clinicians practical clinical tools to help improve the quality of life of their patients with advanced respiratory disease and chronic breathlessness.To outline the evidence base for these interventions with reference to definitive sources.To highlight the importance of person-centred care in people with respiratory disease at all stages of illness.
Why is medical oxygen a challenge for people travelling by air?
Authors: Orritt Rachel , Powell Pippa , Saraiva Isabel 
Published: September 2019

There are currently 3.5 million people in Europe who require medical oxygen, and as life expectancies increase, this figure is likely to grow. At the same time, air travel is becoming more accessible to a wider range of people, as costs of flights fall, and airlines and airports make improvements to the accessibility of their services.People who need medical oxygen to fly experience a wide range of difficulties when planning to travel by plane, and sometimes during or after the flight.A European Commission Regulation (EC No 1107/2006) sets the standard for airlines when it comes to making air travel accessible, but healthcare professionals and oxygen providers can both help patients to navigate the various requirements for using medical oxygen when travelling.In this review, we discuss the journey of the patient planning to travel by air, from initial consultation and fit-to-fly test, through to planning their air travel and oxygen supply, travelling, and arriving at their destination. We also highlight some common problems at each stage and suggest points for healthcare professionals to discuss with patients.Key pointsPatients who require medical oxygen for air travel should begin planning their trip as far in advance as possible.It is very advantageous for healthcare professionals to be able to advise patients on travelling with oxygen and what they need to do.Requirements and policies can vary greatly between airlines, causing problems for patients who are trying to book their flights.Patients or their carers need to be confident operating their oxygen equipment, as the stress of travel and lack of medical assistance on an airplane can put them at risk.Careful arrangements need to be made by the patient to make sure that they have the correct oxygen therapy at their destination, and can access support if they need it.Educational aimsTo understand the process and potential challenges for a patient who requires oxygen to travel by plane.To be confident in discussing air travel with patients who are affected by lung disease.
Revealing the painterly technique beneath the surface of Vermeer’s Girl with a Pearl Earring using macro- and microscale imaging
Authors: Vandivere Abbie , van Loon Annelies , Dooley Kathryn A. , Haswell Ralph , Erdmann Robert G. , Leonhardt Emilien , Delaney John K. 
Published: September 2019

Abstract
The initial steps that Johannes Vermeer took to create Girl with a Pearl Earring (c. 1665) are, partially or completely, hidden beneath the painted surface. Vermeer’s painting technique involved applying underlayers that vary in colour and thickness, on top of which he applied the upper paint layer(s). To investigate the painting’s genesis, the Girl was examined using non-invasive scientific imaging as part of the project The Girl in the Spotlight (2018). This was complemented by the re-examination of existing paint cross-sections using microscopic analytical techniques, including focused ion beam-scanning transmission electron microscopy (FIB-STEM). The weave of the canvas, visible in the X-radiograph, was examined using a new computer-assisted thread-level canvas analysis algorithm to measure the thread spacing and thread angles. The canvas was primed with a warm light grey ground, although probably not by Vermeer himself. The tinted ground provided neutral base tone upon which he began to lay in areas of light and shade in his composition, and some outlines around the figure. Multispectral infrared reflectography( MS-IRR) images in four spectral bands spanning the spectral range from 900 to 2500 nm were collected of the painting at high spatial resolution (50 µm/pixel). Visible 3D digital microscopy visualised the underlayers where Vermeer left them visible around the perimeter of the figure as a contour, and where the upper paint layers are thin. These infrared reflectograms and visible micrographs identified three distinct preparatory phases of Girl with a Pearl Earring: the ground, fine black outlines, and underlayers. Some pentimenti were also detected, including a change in the position of her ear. The combination of examination methods showed that the materials beneath the surface played an important role in establishing the three-dimensional space, fall of light, and the eventual colour in different areas of the painting.
Acute surgical vs non-surgical management for ocular and peri-ocular burns: a systematic review and meta-analysis
Authors: Klifto Kevin M. , Elhelali Ala , Gurno Caresse F. , Seal Stella M. , Asif Mohammed , Hultman C. Scott 
Published: September 2019

Abstract

Background
Burn-related injury to the face involving the structures of the eyes, eyelids, eyelashes, and/or eyebrows could result in multiple reconstructive procedures to improve functional and cosmetic outcomes, and correct complications following poor acute phase management. The objective of this article was to evaluate if non-surgical or surgical interventions are best for acute management of ocular and/or peri-ocular burns.

Methods
This systematic review and meta-analysis compared 272 surgical to 535 non-surgical interventions within 1 month of patients suffering burn-related injuries to 465 eyes, 253 eyelids, 90 eyelashes, and 0 eyebrows and evaluated associated outcomes and complications. The PubMed, Embase, Cochrane Library, Web of Science, and Scopus databases were systematically and independently searched. Patient and clinical characteristics, surgical and medical interventions, outcomes, and complications were recorded.

Results
Eight of the 14,927 studies queried for this study were eligible for the systematic review and meta-analysis, with results from 33 of the possible 58 outcomes and complications using Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) and Cochrane guidelines. Surgery was associated with standard mean differences (SMD) 0.44 greater visual acuity on follow-up, SMD 1.63 mm shorter epithelial defect diameters on follow-up, SMD 1.55 mm greater changes in epithelial diameters from baseline, SMD 1.17 mm2 smaller epithelial defect areas on follow-up, SMD 1.37 mm2 greater changes in epithelial defect areas from baseline, risk ratios (RR) 1.22 greater numbers of healed epithelial defects, RR 11.17 more keratitis infections, and a 2.2 greater reduction in limbal ischemia compared to no surgical intervention.

Conclusions
This systematic review and meta-analysis found that compared to non-surgical interventions, acute surgical interventions for ocular, eyelid, and/or eyelash burns were found to have greater visual acuity on follow-up, shorter epithelial defect diameters on follow-up, greater changes in epithelial diameters from baseline, smaller epithelial defect areas on follow-up, greater changes in epithelial defect areas from baseline, greater numbers of healed epithelial defects, more keratitis infections, and a greater reduction in limbal ischemia, possibility preventing the need of a future limbal stem cell transplantation.