bluuebunny/crossref_metadata_2025_split huggingface dataset:
An immunoregulatory and metabolism-improving injectable hydrogel for cardiac repair after myocardial infarction
Authors: Sun Yage , Zhao Xinrui , Zhang Qian , Yang Rong , Liu Wenguang 
Published: November 2024

Abstract
The hypoxia microenvironment post-myocardial infarction (MI) critically disturbs cellular metabolism and inflammation response, leading to scarce bioenergy supplying, prolonged inflammatory phase and high risk of cardiac fibrosis during cardiac restoration. Herein, an injectable hydrogel is prepared by Schiff base reaction between fructose-1,6-bisphosphate (FBP)-grafted carboxymethyl chitosan (CF) and oxidized dextran (OD), followed by loading fucoidan-coated baicalin (BA)-encapsulated zein nanoparticles (BFZ NPs), in which immunoregulatory and metabolism improving functions are integrally included. The grafted FBP serves to enhance glycolysis and provide more bioenergy for cardiomyocytes survival under hypoxia microenvironment, and elevating cellular antioxidant capacity via pentose phosphate pathway. OD with intrinsic anti-inflammatory effect can induce M2 polarization of macrophages to accelerate inflammatory elimination. While facing the possibility of endothelial-to-mesenchymal transition (EndoMT) caused by excessive expressed TGF-β1 secreted from M2 macrophages, BFZ NPs can target endothelia cells and intracellularly release BA to regulate the level of fatty acid oxidation, resulting in retained endothelial features and decreased risk of cardiac fibrosis. After being injecting the hydrogel into rats’ infarcted cardiac, the 28-day-post surgical outcomes demonstrate its benign effects on restoring cardiac functions and attenuating adverse left ventricular remodeling. This study shows a promising measure for MI treatment with immunoregulating and metabolism regulation comprehensively.
Lipid-core nanocapsules containing simvastatin do not affect the biochemical and hematological indicators of toxicity in rats
Authors: Lorenzoni Ricardo , Davies Samuel , Cordenonsi Leticia Malgarim , Roggia Isabel , Viçosa José Alcides da Silva , Mezzomo Nathana Jamille , de Oliveira Amanda Lima , do Carmo Guilherme Machado , Vitalis Graciela , Gomes Patrícia , Raffin Renata Platcheck , Alves Oswaldo Luiz , Vaucher Rodrigo De Almeida , Rech Virginia Cielo 
Published: November 2024

Abstract
Our research group previously studied the effectiveness of lipid-core nanocapsules (LNC) containing simvastatin (SV-LNC) in treating cognitive impairment in rats. While our results were promising, we needed to evaluate the potential toxicity of the nanoparticles themselves. This study aimed to compare the biochemical and hematological parameters of adult Wistar rats receiving LNC or SV-LNC to those receiving low doses of simvastatin crystals dispersed in a saline solution over 45 days. We discovered that LNC and SV-LNC, which are both nanometers in size with low polydispersity index, negative zeta potential, and high SV encapsulation efficacy, were not more toxic than SV crystals based on various biochemical markers of hepatic, pancreatic, renal, mineral, bony, alkaline phosphatase, glucose, and uric acid damage. Furthermore, LNC exhibited no toxicity for hematological parameters, including red and white blood cell counts. Based on this animal model of toxicological study, our findings suggest that long-term administration of LNC is a safe and promising nanocarrier.
The Molecular Dialogue Between Zymoseptoria tritici and Wheat
Authors: Meile Lukas , Carrasco-López Cristian , Lorrain Cécile , Kema Gert H. J. , Saintenac Cyrille , Sánchez-Vallet Andrea 
Published: November 2024

Zymoseptoria tritici is a highly damaging pathogen that causes high wheat yield losses in temperate climates. Z. tritici emerged during the domestication of wheat in the Fertile Crescent and has been extensively used as a model system for population genetic and genomic studies. New genetic tools and resources have provided a better understanding of the molecular components involved in the wheat- Z. tritici interaction, which is highlighted by the cloning of three wheat resistance genes and four Z. tritici avirulence genes. Despite the considerable progress made in the last few years, the mechanisms that mediate Z. tritici colonization remain largely unknown. In this review, we summarize the latest advances in understanding the molecular components mediating wheat- Z. tritici interactions, and we discuss future research lines to close current knowledge gaps. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .
GATA1‐activated lncRNA OIP5‐AS1 and GAS5 promote pyroptosis to exacerbate asthma through regulating miR‐136‐5p/LIFR axis
Authors: Zhao Suzhi , Huang Sini , Wu Yawei , Yao Xiaozhou , Cai Xingjun 
Published: November 2024

AbstractPyroptosis plays a pivotal role in airway epithelial inflammation during the progression of asthma. This study aimed to explore the influence and mechanisms of opa‐interacting protein 5 antisense RNA1 (OIP5‐AS1) and growth arrest‐specific transcript 5 (GAS5) on pyroptosis in asthmatic models. Pyroptosis was induced in Dermatophagoides pteronyssinus 1 (Der p1)‐exposed 16HBE cells and ovalbumin (OVA)‐challenged rats. Subsequently, pyroptosis and its related molecular mechanisms were investigated. Our results indicated that GATA1, OIP5‐AS1, GAS5, and LIFR were upregulated, while miR‐136‐5p was downregulated in the patients and experimental models of asthma. OIP5‐AS1/GAS5 knockdown repressed NLRP3 inflammasome‐mediated pyroptosis in 16HBE cells. Mechanistically, OIP5‐AS1/GAS5 sponged miR‐136‐5p to enhance LIFR expression and subsequently activated NF‐κB pathway. OIP5‐AS1, GAS5, or LIFR‐mediated induction of pyroptosis was abrogated by miR‐136‐5p mimics or NF‐κB inhibitors (BAY11‐7082). Finally, GATA1 transcriptionally activated OIP5‐AS1/GAS5 to trigger pyroptosis, thereby driving asthma progression in vivo and in vitro. In conclusion, OIP5‐AS1/GAS5 transcriptionally activated by GATA1 promoted NLRP3 inflammasome‐mediated pyroptosis via the modulation of miR‐136‐5p/LIFR/NF‐κB axis and consequently resulted in airway inflammation in asthma. Our results may provide novel therapeutic strategies for asthma.
Minimally Invasive Transthoracic Intramyocardial Cellular Transplantation Under Echocardiographic Guidance for Myocardial Impairment
Authors: Li Sulei , Tao Bo , Liu Junsong , Liu Dongyue , Wang Yujia , Zhang Jibin , Tian Lei , Xu Baixuan , Fang Yan , Wang Yabin , Lou Xin , Zhang Jinming , Lan Feng , Cao Feng 
Published: November 2024

Abstract

Objective:
To explore the approach of minimally invasive transthoracic intramyocardial cellular transplantation under echocardiographic guidance to promote ischemic myocardial repair in a preclinical big-animal study.

Methods:
Female Guangxi Bama miniature pigs (weight: 25–30 kg) were randomly allocated into the sham group, untreated myocardial infarction (MI) group (MI group), the MI and surgical intramyocardial injection (SIM) group (MI-SIM group), and the MI and transthoracic echocardiography-guided percutaneous intramyocardial injection (TTEPIM) group (MI-TTEPIM group) (n = 4 each) using a lottery method. A swine MI model was established in the 3 groups excluding the sham group, and human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CM) labeled with the herpes simplex virus type-1 thymidine kinase reporter gene (hiPS-CMTK+) were transplanted by SIM in MI-SIM group and TTEPIM in MI-TTEPIM group. The operation time, postoperative recovery time of animals and volume of blood loss were collected for comparison between MI-SIM group and MI-TTEPIM group. 9-(4-[18F] fluoro-3-(hydroxymethyl) butyl) guanine positron emission tomography/computed tomography imaging was performed to track the hiPS-CMTK+
in vivo. Cardiac function and morphology were evaluated by echocardiography.

Results:
The operation time and postoperative recovery time of MI-TTEPIM group were significantly shorter than those of MI-SIM group ((28.3 ± 3.6) min vs. (97.0 ± 6.7) min, P < 0.001; (1.3 ± 0.3) d vs. (7.5 ± 0.9) d, P < 0.001). MI-TTEPIM also showed significantly lesser volume of blood loss during cell transplantation than MI-SIM group ((4.3 ± 0.8) mL vs. (47.0 ± 4.1) mL, P < 0.001). The transplanted cells could be traced more accurately in vivo in MI-TTEPIM than in MI-SIM. The circumferential strain of intervention region in the MI-TTEPIM group (–25.07% ± 0.27%) was significantly higher than that of the MI-SIM (–20.39% ± 0.67%) and MI groups (–19.68% ± 0.67%), respectively (P < 0.01).

Conclusion:
A minimally invasive TTEPIM protocol with stem cells for treating the ischemic myocardium was established in this study. Transplantation of hiPS-CMTK+ with this method could promote the recovery of the circumferential strain of the ischemic myocardium. The findings of this study lay a foundation for the clinical transformation of this auxiliary means of treatment in the future.
Global, regional, and national lifetime risk of developing and dying from lung cancer in 2022: A population-based study in 185 countries
Authors: Li Meng , Wen Xin , Liang Xin , Liu Mengwen , Zhang Li , Zheng Rongshou 
Published: November 2024

Abstract

Background:
Lifetime cancer risk is an index that indicates the cumulative probability of cancer at some age during a person’s lifetime. Nevertheless, comparative evaluations regarding the probability of developing lung cancer and dying from the disease among diverse populations at the global, regional, and national levels are scarce.

Methods:
Lung cancer data from 185 countries were obtained from GLOBOCAN 2022, and data on any other cause of death were acquired from the United Nations. The lifetime risks of lung cancer development and death were estimated using adjustment for multiple primary cancers (AMP) method. The lung cancer risks in countries or regions worldwide were compared by region and the Human Development Index (HDI).

Results:
The global lifetime risk of developing and dying from lung cancer in 2022 was 3.49% and 2.69%, respectively. The lifetime probabilities of developing lung cancer in countries/areas with low, medium, high, and very high HDIs were 0.33%, 0.95%, 4.72%, and 5.29%, and dying from lung cancer in low, medium, high and very high HDI countries were 0.30%, 0.86%, 3.69% and 3.92%, respectively. After the age of 40 years, the remaining probability of lung cancer development and death decreased with age, leaving a residual risk of 2.00% and 1.71%, respectively, starting at 70 years.

Conclusions:
The probability of developing lung cancer during one’s lifetime is equivalent to 1 in 28 and 1 in 37 people suffering and dying from lung cancer. The age-related risk of developing and dying of lung cancer varies among geographic locations with different HDIs.
Comparison of Traditional and Intensive Cardiac Rehabilitation on Dietary Behavior and Clinical Risk Factor Outcomes
Authors: Rasmussen Ruth A. , Sisson Susan B. , Baldwin Jonathan D. , Hord Norman , Eliot Kathrin , Anderson Leah , Gowin Mary J. , Scott Brook D. , Wortham Darlene 
Published: November 2024

Purpose:
The objective of this study was to assess differences in dietary behavior and clinical risk factor outcomes and explore predictors of change among patients participating in traditional and Pritikin intensive cardiac rehabilitation (CR).

Methods:
This secondary analysis of cardiac registry data from 2015 to 2021 included patients participating in traditional CR (n = 420) or Pritikin intensive CR (n = 1005) at a single hospital site. Dietary behavior outcomes included the Rate Your Plate measure, while clinical risk factor outcomes included fasting lipids, blood pressure, anthropometrics, functional outcomes, and psychosocial assessment. Analysis of covariance examined a difference in Rate Your Plate scores between CR groups. Multivariate analysis of covariance was used to determine differences in clinical risk factor variables between CR groups. Predictors of change in dietary behavior and clinical risk factors were determined through multiple bivariate linear regression models within each CR group.

Results:
Both CR programs led to significant improvements though more change was observed among Pritikin intensive CR participants in dietary behavior, anthropometrics, and fasting lipids. Status as a current or recent smoker was not a significant predictor of dietary behavior. Male sex, status as a current or recent smoker, beta-blockers, and angiotensin-converting enzyme inhibitors prescribed were not significant predictors of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, or non–high-density lipoprotein cholesterol for traditional and Pritikin intensive CR groups. There were not significant predictors of blood pressure, body mass, or waist circumference.

Conclusion:
Although participation in either CR program yielded cardiometabolic benefits, Pritikin intensive CR patients exhibited significantly greater improvements in dietary behavior and clinical risk factors.
ArtiSential versus conventional laparoscopic colorectal cancer surgery: a multicenter retrospective matched cohort study
Authors: Pyo Dae Hee , Lee Yoon Suk , Min Byung Soh , Lee Jaeim , Kim Chang Hyun , Oh Heung-Kwon , Yoon Yong Sik , Bae Donghwan , Huh Jung Wook 
Published: November 2024

Background:
This study aims to compare outcomes of colorectal cancer surgeries performed using the newly developed articulating laparoscopic instrument, ArtiSential, with those using conventional non-articulating or rigid laparoscopic instruments.

Methods:
This multicenter, retrospective, matched cohort study enrolled patients with colorectal cancer undergoing laparoscopic surgery in seven tertiary referral hospitals from January 2021 to October 2022. A 1:1 propensity score matching was performed between the articulating (Arti-LAP) and conventional (Rigid-LAP) laparoscopic groups. The primary outcome was the overall incidence of short-term complications.

Results:
The study initially comprised 694 and 2008 patients in the Arti-LAP and Rigid-LAP groups, respectively. After matching, each group consisted of 694 patients, with no significant differences in baseline clinical characteristics. The mean operation time was 161±32 min in Arti-LAP and 152±25 min in Rigid-LAP groups (P=0.105). Open conversion rates were 0% (0/694) in Arti-LAP and 1.0% (7/694) in Rigid-LAP groups (P=0.024). Overall morbidity rates were 9.5% (66/694) in Arti-LAP and 12.8% (89/694) in Rigid-LAP (P=0.061), with similar rates of severe morbidities. Specimen quality was acceptable in both groups. Multivariable regression analysis showed that the choice of instrument type (articulating or rigid) was not associated with perioperative morbidity. Despite a short median follow-up period of 19.5 months, no significant difference in disease-free survival was observed between the groups.

Conclusion:
Laparoscopic surgery using ArtiSential was performed safely and effectively for patients with colorectal cancer. Articulating laparoscopic instruments may offer an advantage in reducing open conversion rates.
Successful Treatment of Mycoplasma genitalium Urethritis With High-Dose Tinidazole
Authors: Liscynesky Christina , Lipps Ashley , Bazan Jose A. 
Published: November 2024

Abstract
A 30-year-old male patient with symptomatic Mycoplasma genitalium urethritis failed treatment with oral azithromycin, 2-stage doxycycline-moxifloxacin, and minocycline. Molecular testing confirmed the presence of macrolide resistance mutations. Treatment with oral tinidazole 2 g daily for 7 days resulted in clinical and microbiologic cure.
Parametric analysis of 3D printing parameters on stiffness and hysteresis characteristics of pediatric prosthetic foot coupon samples
Authors: Batley Abigail , Glithro Richard , Montalvão Diogo , Dyer Bryce , Sewell Philip 
Published: November 2024

Background:
This research paper presents a comprehensive parametric study that investigates the influence of various 3D printing parameters on the mechanical properties of a pediatric prosthetic keel using coupon samples.

Objective:
To analyze how 3D printing parameters impact upon on 2 crucial mechanical properties: stiffness and hysteresis.

Methods:
Key 3D printing parameters including the amount of continuous carbon fiber layers, fiber distribution, and matrix fill pattern, are systematically varied, and mechanically tested through compression to analyze the results.

Results:
The results demonstrate the substantial impact that printing parameters have on the mechanical characteristics of 3D-printed pediatric prosthetic feet. Notably, the selection of these parameters for the prosthetic keel plays a pivotal role in shaping the overall performance and functionality of the prosthetic foot, emphasizing the need for precise parameter optimization in pediatric prosthetic design, if 3D printing is the manufacturing process.

Conclusions:
The findings of this study contribute to a better understanding of the manufacturing process for pediatric prosthetic feet via 3D printing and offer valuable insights for optimizing their design. By identifying the ideal combination of 3D printing parameters that yield the desired stiffness and minimize hysteresis, we aim to enhance the performance and comfort of pediatric prosthetic devices, ultimately improving the quality of life for young users.
Nonselective beta blockade enhances gut microbiome diversity in a rodent model of trauma, hemorrhage, and chronic stress
Authors: Munley Jennifer A. , Kelly Lauren S. , Park Gwoncheol , Pons Erick E. , Apple Camille G. , Kannan Kolenkode B. , Bible Letitia E. , Efron Philip A. , Nagpal Ravinder , Mohr Alicia M. 
Published: November 2024

BACKGROUND
Traumatic injury leads to gut dysbiosis with changes in microbiome diversity and conversion toward a “pathobiome” signature characterized by a selective overabundance of pathogenic bacteria. The use of non-selective beta antagonism in trauma patients has been established as a useful adjunct to reduce systemic inflammation. We sought to investigate whether beta-adrenergic blockade following trauma would prevent the conversion of microbiome to a “pathobiome” phenotype.

METHODS
Sprague-Dawley rats (n = 6–8/group) were subjected to routine daily handling (naïve), lung contusion with hemorrhagic shock (LCHS), or LCHS with daily chronic stress (LCHS/CS), each with or without administration of intraperitoneal propranolol (BB) (10 mg/kg/day). Fecal microbiome was measured on Days 0, 7, and 14 using high-throughput 16S rRNA sequencing and QIIME2 bioinformatics analyses. Alpha- and beta-diversity and microbiome composition were assessed with significance defined as *p < 0.05.

RESULTS
Use of propranolol following LCHS or LCHS/CS demonstrated a significant increase in the number of bacterial species (Chao1 index), as well as overall richness and evenness (Shannon index) compared with their untreated counterparts at Day 7. By Day 14, these differences were no longer apparent between BB and untreated groups subjected to LCHS/CS. There was an abundance of commensal bacteria such as Oscillospiraceae and Clostridia in LCHS and LCHS/CS treated with BB after 7 days which persisted at 14 days.

CONCLUSION
These findings suggest a role for beta-antagonism in altering the diversity of the gut microbiome and the need for further studies to elucidate the cellular and molecular mechanisms underlying this intriguing connection of microbiome with trauma and beta-blockade.
Cities beyond proximity
Authors: Hill Dan , Bruno Matteo , Melo Hygor P. M. , Takeuchi Yuichiro , Loreto Vittorio 
Published: November 2024

The concept of ‘proximity-based cities’ has gained attention as a new urban organizational model. Most prominently, the 15-minute city contends that cities can function more effectively, equitably and sustainably if essential, everyday services and key amenities are within a 15-minute walk or cycle. However, focusing solely on travel time risks overlooking disparities in service quality, as the proximity paradigm tends to emphasize the mere presence of an element in a location rather than bringing up more complex questions of identity, diversity, quality, value or relationships. Transitioning to value-based cities by considering more than just proximity can enhance local identity, resilience and urban democracy. Fostering bottom–up initiatives can create a culture of local care and value, while predominantly top–down governing strategies can lead to large inequalities. Balancing these approaches can maximize resilience, health and sustainability. This equilibrium has the potential to accompany sustainable growth, by encouraging the creation of innovative urban solutions and reducing inequalities.
This article is part of the theme issue ‘Co-creating the future: participatory cities and digital governance’.
Functional segregation of cortical hand and speech areas by frequency detuning of an intrinsic motor rhythm
Authors: Anastasopoulou Ioanna , Cheyne Douglas O , Johnson Blake W 
Published: November 2024

ABSTRACTMany decades after Penfield’s (1937) classic depiction of the motor homunculus, it remains unclear how spatially contiguous and interconnected representations within human sensorimotor cortex might separate their activities to achieve the directed and precise control of distinct body regions evident in activities as different as typing and speaking. One long-standing but relatively neglected explanation draws from models of simple physical systems (like swinging pendulums) to posit that small differences in the oscillatory properties of neuronal populations (termed “frequency detuning”) can result in highly effective segregation of their activities and outputs. We tested this hypothesis by comparing the peak frequencies of beta-band (13-30 Hz) motor rhythms measured in a magnetoencephalographic neuroimaging study of finger and speech movements in a group of healthy adults and a group of typically developing children. Our results confirm a peak frequency task difference of about 1.5 Hz in the beta motor rhythms of both left and right hemispheres in adults. A comparable task difference was obtained in children for the left but not for the right hemisphere. These results provide novel support for the role of frequency detuning in the functional organisation of the brain and suggest that this mechanism should play a more prominent role in current models of bodily representations and their development within the sensorimotor cortex.NEW & NOTEWORTHYThis work is the first evidence for frequency detuning of an intrinsic motor rhythm in spatially contiguous regions of primary motor cortex associated with hand and speech movements, supporting a mechanism of functional segregation that has been largely overlooked by current models of motor cortex organization. Our results from children further provide the first evidence for frequency detuning in the developing brain, indicating an early-developing mechanism with later refinements of hemispheric control.