bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface Attachment Enhances the Thermodynamic Stability of Protein L
Authors: Ortega Gabriel , Kurnik Martin , Dauphin‐Ducharme Philippe , Li Hui , Arroyo‐Currás Netzahualcóyotl , Caceres Amanda , Plaxco Kevin W. 
Published: December 2018

AbstractDespite the importance of protein–surface interactions in both biology and biotechnology, our understanding of their origins is limited due to a paucity of experimental studies of the thermodynamics behind such interactions. In response, we have characterized the extent to which interaction with a chemically well‐defined macroscopic surface alters the stability of protein L. To do so, we site‐specifically attached a redox‐reporter‐modified protein variant to a hydroxy‐terminated monolayer on a gold surface and then used electrochemistry to monitor its guanidine denaturation and determine its folding free energy. Comparison with the free energy seen in solution indicates that interaction with this surface stabilizes the protein by 6 kJ mol−1, a value that is in good agreement with theoretical estimates of the entropic consequences of surface‐induced excluded volume effects, thus suggesting that chemically specific interactions with this surface (e.g., electrostatic interactions) are limited in magnitude.
Brückeninstandsetzung, Ertüchtigung und Abdichtung mit Ultrahochfestem‐Faserbeton (UHFB)
Authors: Methner Raphael , Müller Roger 
Published: December 2018

AbstractBei der Instandsetzung und Verstärkung von drei Schweizer Großbrücken auf der Autobahn A4 zwischen Küssnacht und Brunnen wird im Auftrag des Bundesamts für Strassen (ASTRA) Ultrahochfester‐Faserbeton (UHFB) in großem Umfang eingesetzt. Gegenüber klassischen Verstärkungen, z. B. CFK‐Lamellen oder externer Vorspannung, erwies sich die Verstärkung der Stützbereiche mit UHFB als sinnvolle Alternative. Wie durch nichtlineare Berechnungen gezeigt werden konnte, führt dies zu einer Erhöhung der Systemtraglast. Normative Grundlage bildet das SIA‐Merkblatt 2052, welches im März 2016 veröffentlicht wurde. Erstmals (in dieser Größenordnung) wird auf die Anwendung einer konventionellen Brückenabdichtung verzichtet. Damit konnten die Baukosten und die Bauzeit reduziert werden. Neben der Ertüchtigung wird UHFB für die Instandsetzung der Längsträgerstege verwendet.
Determination of olanzapine for therapeutic drug monitoring in schizophrenia patients by LC/MS method
Authors: Albayrak Mevlut , Kadioglu Yucel , Yaman Mehmet Emrah , Senol Onur , Oral Elif 
Published: December 2018

AbstractOlanzapine is an atypical antipsychotic drug from the thienobenzodiazepine family which displays efficacy in patients with schizophrenia and related psychoses. A novel LC/MS method was developed and validated for determination of olanzapine in schizophrenia patients' plasma. A liquid–liquid extraction procedure was carried out using 5 mL diethyl ether–diisopropyl ether mixture (1:1, v/v). Average recovery of the extraction procedure was 94.8%. Chromatographic separation was performed on reversed‐phase C18 column (250 × 2.0 mm, 5 μm) using mixture of deionized water (trifluoro acetic acid 0.1%)–acetonitrile (20:80, v/v) as mobile phase at a flow rate of 1 mL/min. Irbesartan was used as internal standart and total run time was 2.5 min. Mass spectrometric analysis were carried out in selective‐ion montoring mode, and detected olanzapine at m/z 313.1 and IS at m/z 429.4 in all forms of the ions. The calibration curve of olanzapine was linear in the range 2–300 ng/mL (r2 > 0.9993). The interday and intraday precisions (RSD) were <7.55%, and accuracy was >7.59% (n = 6). The proposed study was successfully validated with respect to the US Food and Drug Administration guidelines.
Incremental cost and length of stay associated with postprocedure delirium in transcatheter and surgical aortic valve replacement patients in the United States
Authors: Potter Brian J. , Thompson Christin , Green Philip , Clancy Seth 
Published: December 2018

AbstractObjectivesTo explore the impact of post‐procedure delirium on resource utilization following transcatheter and surgical aortic valve replacement (TAVR and SAVR, respectively).BackgroundPostprocedure delirium is associated with worse long‐term survival after TAVR and SAVR. However, its effect on resource utilization has been understudied.MethodsUsing the 2015 Medicare Provider Analysis and Review File (MedPAR), we retrospectively analyzed elderly (≥80 years) Medicare beneficiaries receiving either SAVR or endovascular TAVR in the United States. Multivariate regression models estimating hospitalization cost and length of stay (LoS) were adjusted for patient demographics, comorbidities, and nondelirium complications.ResultsA total of 21,088 discharges were available for analysis (12,114 TAVR and 8,974 SAVR). TAVR patients were older (87 ± 3.8 vs. 84 ± 2.7 years; P < 0.001) with a higher comorbidity burden (Charlson index 3.0 ± 1.8 vs. 2.1 ± 1.7; P < 0.0001). Despite this, fewer TAVR patients (1.6%) experienced postoperative delirium during the index hospitalization compared to surgical patients (3.6%; P < 0.0001). Delirium was associated with a 4.16 [3.51–4.81] day longer hospital LoS and $15,592 ($12,849–$18,334) higher incremental hospitalization cost. When stratified by treatment approach, the adjusted incremental cost of delirium was +$13,862 ($9,431–$18,292) with TAVR and +$16,656 ($13,177–$20,136) with SAVR with an additional hospital LoS of +3.39 (2.34–4.43) days and +4.63 (3.81–5.45) days for TAVR and SAVR, respectively.ConclusionsPostprocedure delirium is associated with significantly increased hospitalization costs and LoS following AVR. TAVR was associated with a lower postoperative delirium rate compared to SAVR. Post‐TAVR delirium may be associated with less resource consumption than post‐SAVR delirium.
Straightforward Ball‐Milling Access to Dinucleoside 5′,5′‐Polyphosphates via Phosphorimidazolide Intermediates
Authors: Appy Lucie , Depaix Anaïs , Bantreil Xavier , Lamaty Frédéric , Peyrottes Suzanne , Roy Béatrice 
Published: December 2018

AbstractA solvent‐assisted mechanochemical approach to access symmetrical and mixed dinucleoside 5,5′‐polyphosphates is reported. Under ball‐milling conditions, nucleoside 5′‐monophosphates were quantitatively activated using 1,1′‐carbonyldiimidazole, forming their phosphorimidazolide derivatives. The addition of a nucleoside 5′‐mono‐, di‐ or triphosphate directly led to the formation of the corresponding dinucleotides. Benefits of the reported one‐pot method include the use of unprotected nucleotides in their sodium or acid form, activation by the eco‐friendly 1,1′‐carbonyldiimidazole, non‐dry conditions, short reaction time, high conversion rates, and easy setup and purification. This work offers new perspectives for the synthesis of nucleotide conjugates and analogues, combining the phosphorimidazolide approach and milling conditions.
Cutting and nesting system of cloud manufacturing based on SOA
Authors: Li Bo , Wang Shi 
Published: December 2018

SummaryCutting and nesting are common problems in production manufacturing. However, most existing research works focus on the optimization algorithm rather than commercialized application software system. A kind of cutting and nesting system for cloud manufacturing based on SOA is proposed in the paper, which deploys nesting algorithm distributedly to multi‐core computer cluster so as to provide the cutting and nesting service for numerous small and medium‐sized enterprises through uniform network service and to realize the integration with existing enterprise system based on SOA. Firstly, the system structure is introduced in the paper. Then, distributed scheduling algorithm, service architecture process, and relevant service interface of the system are stated. Finally, a typical application case for the system is established.
Contribution of Adipose Tissue Inflammation to the Development of Type 2 Diabetes Mellitus
Authors: Burhans Maggie S. , Hagman Derek K. , Kuzma Jessica N. , Schmidt Kelsey A. , Kratz Mario 
Published: December 2018

ABSTRACTThe objective of this comprehensive review is to summarize and discuss the available evidence of how adipose tissue inflammation affects insulin sensitivity and glucose tolerance. Low‐grade, chronic adipose tissue inflammation is characterized by infiltration of macrophages and other immune cell populations into adipose tissue, and a shift toward more proinflammatory subtypes of leukocytes. The infiltration of proinflammatory cells in adipose tissue is associated with an increased production of key chemokines such as C‐C motif chemokine ligand 2, proinflammatory cytokines including tumor necrosis factor α and interleukins 1β and 6 as well as reduced expression of the key insulin‐sensitizing adipokine, adiponectin. In both rodent models and humans, adipose tissue inflammation is consistently associated with excess fat mass and insulin resistance. In humans, associations with insulin resistance are stronger and more consistent for inflammation in visceral as opposed to subcutaneous fat. Further, genetic alterations in mouse models of obesity that reduce adipose tissue inflammation are—almost without exception—associated with improved insulin sensitivity. However, a dissociation between adipose tissue inflammation and insulin resistance can be observed in very few rodent models of obesity as well as in humans following bariatric surgery‐ or low‐calorie‐diet‐induced weight loss, illustrating that the etiology of insulin resistance is multifactorial. Taken together, adipose tissue inflammation is a key factor in the development of insulin resistance and type 2 diabetes in obesity, along with other factors that likely include inflammation and fat accumulation in other metabolically active tissues. © 2019 American Physiological Society.Compr Physiol9:1‐58, 2019.
Elevated circulating cathepsin S levels are associated with metabolic syndrome in overweight and obese individuals
Authors: Chen Lili , Lu Bin , Yang Yehong , Zhang Weiwei , Wang Xuanchun , Zhou Houguang , Wen Jie , Yang Zhen , Hu Renming 
Published: December 2018

AbstractObjectiveCathepsin S is highly expressed in subcutaneous and visceral adipose tissue. Cathepsin S correlates with central obesity and contributes to the formation and progression of atherosclerosis. Here, we sought to evaluate the association of serum cathepsin S with metabolic syndrome (MS) in overweight and obese Chinese adults.MethodsWe evaluated serum cathepsin S levels in a cross‐sectional sample of 781 overweight and obese Chinese adults by ELISA. Glucose, insulin, lipid profile, inflammatory markers, and adipokines were also measured.ResultsCathepsin S was significantly associated with BMI, waist circumference, waist‐to‐hip ratio, fasting glucose, fasting insulin, the homeostatic model assessment of insulin resistance (HOMA‐IR), systolic blood pressure, C‐reactive protein (CRP), triglycerides, and HDL cholesterol (all P < 0.05). Plasma cathepsin S levels increased significantly (P = 0.045 for trend) with increasing numbers of MS components after adjustment for potential confounders. In the highest cathepsin S quartile, the MS risk was significantly higher (odds ratio 2.30; 95% confidence interval, 1.89‐2.78) than in the lowest quartile after adjustment for age, gender, alcohol consumption, smoking, education, physical activity, self‐reported CVD, and family history of diabetes. This association remained strong (odds ratio 1.97; 95% confidence interval, 1.72‐2.48) after controlling further for CRP, adiponectin, HOMA‐IR, and BMI.ConclusionsElevated circulating cathepsin S concentrations are strongly and independently associated with MS in overweight and obese Chinese adults. Prospective studies are needed to establish the role of cathepsin S in the development of MS.
Mesozoic basin evolution of the East China Sea Shelf and tectonic system transition in Southeast China
Authors: Yang Changqing , Han Baofu , Yang Chuansheng , Yang Yanqiu , Sun Jin , Yu Fusheng 
Published: December 2018

AbstractThe East China Sea Shelf Basin (ECSSB) is located on the south‐eastern edge of the Eurasian Plate. The tectono‐evolution and dynamic mechanism of the ECSSB are related to the collision of the Pacific Plate with the Eurasian Plate, and the remote pushing effect of the Indo‐Australian Plate remains a hot topic of debate. Based on the latest survey data, this paper mainly focuses on the evolution of the Mesozoic basin in the East China Sea Shelf by means of assessing the regional tectonic background, conducting structural physical simulation experiments, and applying balanced geological section recovery technology to discuss the processes of dynamic transition in Southeast China. Our data suggest that the ECSSB experienced an evolutionary sequence involving the pre‐Late Triassic passive continental margin; the Late Triassic–Middle Jurassic active continental margin extrusion‐depression; the extrusional stress from the low‐angle subduction of the Izanaki Plate under the Eurasian Plate; the late Early Cretaceous to Late Cretaceous active continental marginal extension faulted basin, during which the extensional stress originated from the lithospheric thinning and palaeoenvironment resulting from the subduction of the Palaeo‐Pacific Plate under the Eurasian Plate; and the Palaeogene back‐arc extension faulted sags. The transition time from the E‐W‐trending Palaeo‐Tethys tectonic system to the NE‐trending Palaeo‐Pacific tectonic system in Southeast China occurred at the end of the Middle Triassic. The low‐angle subduction and withdrawal of subduction of the Palaeo‐Pacific Plate probably represented the deep geological processes of the Mesozoic in Southeast China.
Compatibility of CO2 laser surgery and fluorescence detection in head and neck cancer cells
Authors: Odenthal Julia , Friedl Peter , Takes Robert P. 
Published: December 2018

AbstractBackgroundSurgical treatment of cancer requires tumor excision with emphasis on function preservation which is achieved in (early stage) laryngeal cancer by transoral carbon dioxide (CO2) laser surgery. Whereas conventional laser surgery is restricted by the surgeon's visual recognition of tumor tissue, new approaches based on fluorescence‐guided surgery (FGS) improve the detection of the tumor and its margin. However, it is unclear whether fluorophores are compatible with high‐power laser application or whether precision is compromised by laser‐induced bleaching of the dye.MethodsWe applied topology‐controlled 3D laser resection of fluorescent tumors cell in vitro and laser‐induced autofluorescence analysis ex vivo.ResultsLaser‐induced bleaching of fluorescent dyes in the visible and near‐infrared light spectrum (650‐900 nm) ranges below the resolution range of operation microscopes. Furthermore, specific fluorescent signals in an FGS mouse model is 104 higher than laser‐induced autofluorescence in mouse tissue.ConclusionLaser‐induced lateral photobleaching is negligible indicating a path forward for fluorescence‐guided laser surgery in head and neck cancer.
The Role of Free Tissue Transfer in the Management of Chronic Frontal Sinus Osteomyelitis
Authors: Rimmer Ryan A. , Duffy Alexander N. , Knops Alexander M. , Rabinowitz Mindy R. , Koszewski Ian J. , Rosen Marc R. , Ortlip Timothy , Heffelfinger Ryan N. , Garcia Hermes G. , Evans James J. , Nyquist Gurston G. , Curry Joseph M. 
Published: December 2018

Objectives/HypothesisChronic frontal sinus infection is managed with a combination of medical and surgical interventions. Frontal bone osteomyelitis due to recurrent infection following trauma or prior open surgery may require more significant debridement. Free tissue transfer may allow for extensive debridement with replacement of tissue, and definitive eradication of osteomyelitis.Study DesignRetrospective chart review.MethodsPatients undergoing free flap obliteration of the frontal sinus for frontal bone osteomyelitis at a single institution were included in the study. Clinical, radiologic, and surgical data were collected. Surgeries before and after free flap obliteration were compared by Wilcoxon signed rank test.ResultsFifteen patients were identified; however, one patient had less than 6 months of follow‐up and was excluded from analysis. Of the remaining 14 patients, mean follow‐up duration was 26 months (range, 6–120 months). Mean number of surgeries prior to free flap was 3.7 (range, 1–8 surgeries). Free flap obliteration resolved chronic frontal sinusitis in all patients. Two patients experienced postoperative infection, and the overall complication rate was 29%. Eight patients underwent cranioplasty (six immediate, two delayed) without complication. All patients received planned courses of postoperative antibiotics. A statistically significant decrease in the number of surgeries after free flap obliteration was observed P ≤ .01).ConclusionsExtensive debridement followed by free tissue transfer and antibiotics offers a definitive treatment for complicated, recurrent frontal osteomyelitis. Simultaneous cranioplasty provides immediate protective and aesthetic benefit without complication. Consideration should be given for free tissue transfer and cranioplasty earlier in the algorithm for treatment of refractory frontal sinus osteomyelitis.Level of Evidence4Laryngoscope, 129:1497–1504, 2019
The Reaction of Poly(Vinyl Amine) with Acetone in Water
Authors: Trommler Katja , Walther Tina , Seifert Susan , Seifert Andreas , Hähnle Hans‐Joachim , Anders Susann , Kroll Lothar , Spange Stefan 
Published: December 2018

AbstractPoly(vinyl amine) (PVAm) reacts with acetone in aqueous solution. It generates imine and aminal moieties along the PVAm backbone. The molecular structure of acetone‐modified PVAm is confirmed by liquid 1H and 13C as well as solid state 13C NMR and ATR‐FTIR spectroscopies. Model compounds produced from 1,3‐diaminopropane with acetone in chloroform are used to assign the solid state 13C NMR signals of the modified polymer. Quantitative elemental analysis of acetone‐modified PVAm samples supports the analytical results. The mechanism of the imine and aminal formation is discussed with regard to the anomeric stabilization of the incipient hemiaminal intermediate. The rapid and unexpectedly favorable formation of PVAm acetone hemiaminal, acetone imine, and aminal formation has implications for the conduct of PVAm research and even the interpretation of prior published results. As acetone was often used in the past to precipitate waterborne PVAm derivatives, this finding has a severe impact on the interpretation of research results. The consequences and the revised interpretation of selected publications are discussed.
The vaginal distention model in mice is not a reliable model of simulated birth trauma‐induced stress urinary incontinence
Authors: El Hassan Ramzi , Galante Alex , Kavran Michael , Ganocy Stephen , Khalifa Ahmad O. , Hijaz Adonis 
Published: December 2018

AimsVaginal distention (VD) is a validated model of birth‐related trauma in rats. Recently a mouse VD model was reported. Our study was originally conducted to evaluate the impact of age on VD in mice. This manuscript describes the study and reports on the lack of reproducibility of VD models in mice.MethodsWe utilized female C57BL/6 mice. A total of 190, 12‐weeks old mice, were randomized into VD and sham groups. We inflated a modified Foley's balloon with 0.3 mL for 1 h inside the mice vagina. Afterwards, we measured the leak point pressure (LPP) at defined timepoints (0, 4, 10, 20, or 40 days). We randomized another 190, 40‐week old, C57BL/6 mice into either VD or sham groups. We used an extra 20 mice as age − matched controls.ResultsIn both 12 and 40 weeks‐old mice, LPP was significantly decreased versus the negative controls at day 0. Additionally, in both 12 and 40 weeks‐old mice, the decrease in LPP was significantly higher in the VD group compared to the sham group at day 0. However, the LPP results were comparable between VD and sham at any other time point thereafter. Furthermore, there was no significant change in LPP values between instrumented (VD and sham) mice and control mice at any time after day 0.ConclusionsThe VD models previously described is not a reproducible model for the study of VD with large number of mice. Our results, unfortunately, do not support its use to study VD injury in mice.
Gold‐nanoparticle‐coated magnetic beads for concentration and ionization of analytes for laser desorption/ionization mass spectrometry
Authors: Kim Moon‐Ju , Park Jong‐Min , Noh Joo‐Yoon , Yun Tae Gyeong , Kang Min‐Jung , Pyun Jae‐Chul 
Published: December 2018

RationaleMagnetic particles coated with gold nanoparticles (Au NPs) (Au‐MAGs) were developed and used (1) for sample concentration and (2) as a solid matrix for laser adsorption/desorption mass spectrometry (LDI‐MS).MethodsThe Au‐MAGs were prepared by (1) coating polystyrene on iron oxide NPs (PS‐MNPs), (2) coating poly‐l‐lysine on the PS‐MNPs (PLL‐coated PS‐MNPs), and (3) coating negatively charged Au NPs on the PLL‐coated PS‐MNPs (Au‐MAGs).ResultsThe Au‐MAGs were used to concentrate the target analyte by means of electrostatic interactions between the positively charged GHP9 and the negatively charged Au‐MAGs as well as selective interactions (such as gold–sulfur interactions) between glutathione (GSH) and Au‐MAGs. Then, the concentrated analyte could be ionized for LDI‐MS.ConclusionsThe Au‐MAGs were demonstrated (1) to concentrate the target analyte in a sample solution, tested by electrostatic interactions and selective interactions between gold and sulfur and (2) to ionize the concentrated analyte for LDI‐MS.
Investigating the vulnerability of nonductile reinforced concrete columns in moderate seismic regions to gravity load collapse
Authors: Mehmood Tahir , Rodsin Kittipoon , Warnitchai Pennung , Kolozvari Kristijan 
Published: December 2018

SUMMARYReinforced concrete (RC) columns are important components of the lateral load‐resisting structural system of RC buildings. In moderate seismic regions, where stringent seismic reinforcement detailing requirements are usually not considered, RC columns are categorized as nonductile. Postearthquake studies have shown that gravity load collapse of RC columns can trigger the progressive collapse of RC buildings. The current study presents the seismic response behavior of nonductile RC columns in moderate seismic regions, with a particular focus on gravity load collapse. Six RC columns, three with lap splices and three without lap splices with variable aspect ratios, were tested under reversed cyclic loading. Experimental results show that the column failing in shear could tolerate the maximum drift in order of 2.7–3.5%, whereas the columns failed in flexural mode could achieve the maximum drift of 4.5%. For the columns with lap splice, the lateral strength was significantly degraded, but all spliced columns could sustain gravity load even when their displacements were more than 4% drift. This very large drift without axial collapse, observed in the current study, is associated with the splice slip causing the large rotation just above the splice region.