bluuebunny/crossref_metadata_2025_split huggingface dataset:
Daphnetin attenuates LPS‐induced osteolysis and RANKL mediated osteoclastogenesis through suppression of ERK and NFATc1 pathways
Authors: Wu Zuoxing , Wu Hailun , Li Chen , Fu Fangsheng , Ding Jiaxin , Shao Siyuan , Li Kai , Yu Xiao , Su Yuangang , Liang Jiamin , Lin Xixi , Yuan Guixin , Zhou Juan , Song Fangming , Zhao Jinmin , Xu Jiake , Liu Qian , Xu Feng 
Published: February 2019

AbstractAseptic prosthetic loosening and periprosthetic infection resulting in inflammatory osteolysis is a leading complication of total joint arthroplasty (TJA). Excessive bone destruction around the bone and prosthesis interface plays a key role in the loosening prostheses leading to revision surgery. The bacterial endotoxins or implant‐derived wear particles‐induced inflammatory response is the major cause of the elevated osteoclast formation and activity. Thus, agents or compounds that can attenuate the inflammatory response and/or inhibit the elevated osteoclastogenesis and excessive bone resorption would provide a promising therapeutic avenue to prevent aseptic prosthetic loosening in TJA. Daphnetin (DAP), a natural coumarin derivative, is clinically used in Traditional Chinese Medicine for the treatment of rheumatoid arthritis due to its anti‐inflammatory properties. In this study, we report for the first time that DAP could protect against lipopolysaccharide‐induced inflammatory bone destruction in a murine calvarial osteolysis model in vivo. This protective effect of DAP can in part be attributed to its direct inhibitory effect on RANKL‐induced osteoclast differentiation, fusion, and bone resorption in vitro. Biochemical analysis found that DAP inhibited the activation of the ERK and NFATc1 signaling cascades. Collectively, our findings suggest that DAP as a natural compound has potential for the treatment of inflammatory osteolysis.
Allogeneic hematopoietic cell transplantation in Farber disease
Authors: Ehlert Karoline , Levade Thierry , Di Rocco Maja , Lanino Edoardo , Albert Michael H. , Führer Monika , Jarisch Andrea , Güngör Tayfun , Ayuk Francis , Vormoor Josef 
Published: February 2019

AbstractBackgroundFarber disease (FD) is a rare, lysosomal storage disorder caused by deficient acid ceramidase activity. FD has long been considered a fatal disorder with death in the first three decades of life resulting either from respiratory insufficiency as a consequence of airway involvement or from progressive neurodegeneration because of nervous system involvement. Peripheral symptoms associated with FD, including inflammatory joint disease, have been described to improve relatively rapidly after hematopoietic cell transplantation (HCT).AimsTo evaluate the disease‐specific status and limitations in the long‐term follow‐up after HCT, investigate genotype/phenotype correlations and the benefit of allogeneic HCT in FD patients with nervous system involvement.Patients and methodsTransplant‐ and disease‐related information of ten FD patients was obtained by using a questionnaire, physicians' letters and additional telephone surveys. ASAH1 gene mutations were identified to search for genotype/phenotype correlations.ResultsAfter mainly busulfan‐based preparative regimens, all patients engrafted with one late graft loss. The inflammatory symptoms resolved completely in all patients. Abnormal neurologic findings were present pre‐transplant in 4/10 patients, post‐transplant in 6/10 patients. Mutational analyses revealed new mutations in the ASAH1 gene and a broad diversity of phenotypes without a genotype/phenotype correlation. With a median follow‐up of 10.4 years, overall survival was 80% with two transplant‐related deaths.ConclusionAllogeneic HCT leads to complete and persistent resolution of the inflammatory aspects in FD patients. It appears to have no beneficial effect on progression of nervous system involvement. New mutations in the acid ceramidase gene were identified. A genotype/phenotype correlation could not be established.
Human adenovirus replication and persistence in hypertrophic adenoids and palatine tonsils in children
Authors: Proenca‐Modena José Luiz , de Souza Cardoso Ricardo , Criado Miriã Ferreira , Milanez Guilherme Paier , de Souza William Marciel , Parise Pierina Lorencini , Bertol Jéssica Wildgrube , de Jesus Bruna Lais Santos , Prates Mirela Cristina Moreira , Silva Maria Lúcia , Buzatto Guilherme Pietrucci , Demarco Ricardo Cassiano , Valera Fabiana Cardoso Pereira , Tamashiro Edwin , Anselmo‐Lima Wilma Terezinha , Arruda Eurico 
Published: February 2019

AbstractThe role of human adenovirus (HAdV) infection in different acute diseases, such as febrile exudative tonsillitis, conjunctivitis, and pharyngoconjunctival fever is well established. However, the relationships, if any, of HAdV persistence and reactivation in the development of the chronic adenotonsillar disease is not fully understood. The present paper reports a 3‐year cross‐sectional hospital‐based study aimed at detecting and quantifying HAdV DNA and mRNA of the HAdV hexon gene in adenoid and palatine tonsil tissues and nasopharyngeal secretions (NPS) from patients with adenotonsillar hypertrophy or recurrent adenotonsillitis. HAdV C, B, and E were detectable in nearly 50% of the patients, with no association with the severity of airway obstruction, nor with the presence of recurrent tonsillitis, sleep apnea or otitis media with effusion (OME). Despite the higher rates of respiratory viral coinfections in patients with HAdV, the presence of other viruses, including DNA and RNA viruses, had no association with HAdV replication or shedding in secretions. Higher HAdV loads in adenoids showed a significant positive correlation with the presence of sleep apnea and the absence of OME. Although this study indicates that a significant proportion (~85%) of individuals with chronic adenotonsillar diseases have persistent nonproductive HAdV infection, including those by HAdV C, B, and E, epithelial and subepithelial cells in tonsils seem to be critical for HAdV C production and shedding in NPS in some patients, since viral antigen was detected in these regions by immunohistochemistry in four patients, all of which were also positive for HAdV mRNA detection.
A novel diffraction tomographic algorithm with narrow observation angle
Authors: Yang Chunxia , Zhang Jian , Tong Meisong 
Published: February 2019

AbstractConventional diffraction tomography (DT) can be used to reconstruct objects with 360° coverage of observation angle. But solving inverse problem could be challenging when the full angle data are not available. In this paper, a novel diffraction tomographic algorithm with extrapolation method is proposed to detect two‐dimensional (2D) dielectric objects in cases where the observation stations are far away from the search domain and the observation angles are limited to a narrow range. Extrapolation is introduced to obtain more data in the frequency domain. Using this algorithm, all locations, shapes, and dielectric properties of targets can be reconstructed under 90° or even narrower observation angle. Due to the use of fast Fourier transform in the implementation, this algorithm is very fast although many iterations of extrapolation are needed. Images can be reconstructed with nearly real‐time performance. Simulation results are given to show the validity of the algorithm.
Optimising proliferation and migration of mesenchymal stem cells using platelet products: A rational approach to bone regeneration
Authors: Moisley Katrina M. , El‐Jawhari Jehan J. , Owston Heather , Tronci Giuseppe , Russell Stephen J. , Jones Elena A. , Giannoudis Peter V. 
Published: February 2019

ABSTRACTThis study investigates how mesenchymal stem cell's (MSCs) proliferation and migration abilities are influenced by various platelet products (PP). Donor‐matched, clinical‐, and control laboratory‐standard PPs were generated and assessed based on their platelet and leukocyte concentrations. Bone marrow derived MSCs were exposed to these PP to quantify their effect on in vitro MSC proliferation and migration. An adapted colony forming unit fibroblast (CFU‐F) assay was carried out on bone marrow aspirate using clinical‐standard PP‐loaded electrospun poly(ϵ‐caprolactone) (PCL) membrane to mimic future clinical applications to contain bone defects. Clinical‐standard PP had lower platelet (2.5 fold, p < 0.0001) and higher leukocyte (14.1 fold, p < 0.0001) concentrations compared to laboratory‐standard PP. It induced suboptimal MSC proliferation compared to laboratory‐standard PP and fetal calf serum (FCS). All PP induced significantly more MSC migration than FCS up to 24 h. The removal of leukocytes from PP had no effect on MSC proliferation or migration. The PP‐loaded membranes successfully supported MSC colony formation. This study indicates that platelet concentrations in PP impact MSC proliferation more than the presence of leukocytes, whilst MSC migration in response to PP is not influenced by platelet or leukocyte numbers. Clinical‐standard PP could be applied alongside manufactured membranes in the future treatment of bone reconstruction. © 2019 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res 37:1329–1338, 2019.
Parenteral Provision of Micronutrients to Adult Patients: An Expert Consensus Paper
Authors: Blaauw Renée , Osland Emma , Sriram Krishnan , Ali Azmat , Allard Johane P. , Ball Patrick , Chan Lingtak‐Neander , Jurewitsch Brian , Logan Coughlin Kathleen , Manzanares William , Menéndez Ana Maria , Mutiara Rina , Rosenfeld Ricardo , Sioson Marianna , Visser Janicke , Berger Mette M. 
Published: February 2019

AbstractBackgroundMicronutrients, an umbrella term used to collectively describe vitamins and trace elements, are essential components of nutrition. Those requiring alternative forms of nutrition support are dependent on the prescribed nutrition regimen for their micronutrient provision. The purpose of this paper is to assist clinicians to bridge the gap between the available guidelines’ recommendations and their practical application in the provision of micronutrients via the parenteral route to adult patients.MethodsBased on the available evidenced‐based literature and existing guidelines, a panel of multidisciplinary healthcare professionals with significant experience in the provision of parenteral nutrition (PN) and intravenous micronutrients developed this international consensus paper.ResultsThe paper addresses 14 clinically relevant questions regarding the importance and use of micronutrients in various clinical conditions. Practical orientation on how micronutrients should be prescribed, administered, and monitored is provided.ConclusionMicronutrients are a critical component to nutrition provision and PN provided without them pose a considerable risk to nutrition status. Obstacles to their daily provision—including voluntary omission, partial provision, and supply issues—must be overcome to allow safe and responsible nutrition practice.
Interspecific competition changes nutrient : nutrient ratios of weeds and maize
Authors: da Conceição de Matos Christiano , da Silva Teixeira Rafael , da Silva Ivo Ribeiro , Costa Maurício Dutra , da Silva Antonio Alberto 
Published: February 2019

AbstractThe elemental ratios of plant tissues are associated with the adaptive and competitive success of a plant species in an ecosystem. So far, no study has evaluated if and how crop–weed competition influences the elemental ratios of competing populations, although such information is important to understand weed infestation dynamics and to improve weed management in agroecosystems. The objective of this study was to analyze weed–crop elemental ratios during interspecific competition between weeds and crops in greenhouse experiments. For this, maize (Zea mays L.) and the weeds Amaranthus viridis L, Bidens pilosa L., and Ipomoea grandifolia (Dammer) O'Donell were grown under seven treatments: maize and weed monocultures, and maize in competition with weeds. Competition between plants practically did not influence growth and nutrient contents of maize but reduced weed growth and nutrient uptake. Maize showed few changes in elemental ratios. In contrast, B. pilosa and I. grandifolia were very sensitive to competition and showed significant increases in C : N, C : P, C : K, N : P, and N : K ratios when grown with maize. A. viridis showed low flexibility of nutrient : nutrient ratios under the same competitive pressure as that faced by B. pilosa and I. grandifolia. The interspecific competition led to increases only in the C : P ratio of A. viridis shoots. Therefore, interspecific competition changes the elemental ratios, mainly of the weeds, and the magnitude of this change is dependent on the plant species involved. Interspecific competition changes plant biomass quality (higher C : nutrient ratios), mainly for B. pilosa and I. grandifolia.
Dry matter production, nutrient accumulation, and nutrient partitioning of barley
Authors: Rogers Christopher W. , Dari Biswanath , Hu Gongshe , Mikkelsen Robert 
Published: February 2019

AbstractTotal dry matter (TDM) and nutrient accumulation, nutrient partitioning, and cumulative growing degree days at the time of maximum nutrient accumulation for two‐row spring barley (Hordeum vulgare L.) are not well quantified under high‐yielding irrigated conditions common in the semi‐arid western United States. Thus, five cultivars of barley were grown under irrigated conditions on a loam soil in the 2015 and 2016 growth seasons to determine these factors. Total nutrient accumulation was greatest at either the soft dough or maturity stage where specific nutrients were greater at one stage as compared to the other. Mean N accumulation was greatest at the soft dough stage (256 kg ha−1) where the regression model accounted for 80% of the variation in the data. Additionally, spike N increased from 91 to 105 kg ha−1 from soft dough to maturity. Specific nutrients (e.g., K) had significantly greater plant (i.e., culms plus leaves) accumulation between soft dough and maturity, 253 and 172 kg ha−1, respectively, where the spike at the same growth stages had an accumulation of 37 and 42 kg ha−1, respectively. In contrast, other nutrients (e.g., P) were remobilized to the spike as noted by the increase from 14 kg ha−1 at soft dough to 26 kg ha−1 at maturity. In addition to nutrient partitioning, linear regressions resulted in well‐correlated models between TDM and total nutrient accumulation (R2 = 0.35–0.88) for measured nutrients. Results from the current study provide critical data on nutrient accumulation as well as regression models for two‐row barley under high‐yielding conditions. This information can be used to improve harvest decisions as well as more accurately predict nutrient cycling in barley cropping systems.
Coffee silverskin as nutraceutical ingredient in yogurt: its effect on functional properties and its bioaccessibility
Authors: Bertolino Marta , Barbosa‐Pereira Letricia , Ghirardello Daniela , Botta Cristian , Rolle Luca , Guglielmetti Alessandro , Borotto Dalla Vecchia Stefania , Zeppa Giuseppe 
Published: February 2019

AbstractBACKGROUNDSilverskin is a by‐product obtained from coffee roasting. It is characterized by a high content of dietary fibre, phenolic compounds and caffeine. The aim of this study was to assess the silverskin obtained from two species of Coffea (Arabica and Robusta) at three percentages (2%, 4%, or 6%) into cow whole‐milk yogurt to raise the nutraceutical value of the products and to verify the bioaccessibility of bioactive compounds during the shelf‐life of 3 weeks.RESULTSThe amount and origin of silverskin significantly influenced all the physicochemical parameters. Concerning the bioactive compounds, the highest levels were observed in yogurt supplemented with 6% of silverskin. Between the coffee species, Arabica yielded the highest 5‐caffeoylquinic acid content and the strongest antioxidant activity, whereas Robusta gave the highest caffeine content.The digestion increased antioxidant activity in the yogurt, possibly because of greater accessibility of compounds.CONCLUSIONThe results obtained highlighted that silverskin can be used in yogurt production to increase the nutraceutical value of the products and that the bioactive compounds are bioaccessible during the digestion process. The characteristics and bioaccessibility of the resulting yogurt were strongly correlated with the coffee species and with the percentage added. © 2019 Society of Chemical Industry
Treatment sequencing for simultaneous colorectal liver metastases
Authors: Krell Robert W , D'Angelica Michael I 
Published: February 2019

AbstractUp to 25% of patients with colorectal cancer present with simultaneous metastases and the liver is frequently the only metastatic site. This review will review treatment sequence planning considerations—including metastatic burden, primary tumor site, chemotherapy response, and ability to perform minimally invasive surgery—for patients with simultaneous resectable colorectal liver metastases. In addition, this review will address conversion chemotherapy, combined vs staged surgeries, and their possible sequences.
A novel strategy to utilize ethylene glycol‐ionic liquids for the selective precipitation of polysaccharides
Authors: Zhao Xiaoyong , Ma Ge , Wu Datong , Cai Pengfei , Pan Yuanjiang 
Published: February 2019

AbstractIn the present work, three hydrophilic ionic liquids based on the combination between imidazolium cations attached with ethylene glycol polymers of various lengths and hexafluorophosphate anion were designed and synthesized for the separation of polysaccharides. By employing dextran 100 kDa as model compound, the effects of ionic liquid content, solvent/anti‐solvent volume, and temperature on its recovery efficiency were investigated systematically. The ability of these ionic liquids to precipitate dextran 100 kDa, increases with the elongation of ethylene glycol polymer chain. The established ionic liquid‐based precipitation system was successfully applied to selectively precipitate polysaccharides from water extracts of three traditional Chinese medicines and the precipitation could be achieved in about 15 min. In addition, the different precipitation responses of acidic, neutral, and basic polysaccharides in the ionic liquid‐based precipitation system and theoretical calculations both suggested that the selective precipitation of polysaccharides was probably mediated by interaction between ionic liquids and polysaccharides. The proposed strategy facilitated the isolation and purification of polysaccharides and may trigger a novel application of ionic liquids in carbohydrate research.
LC method for the direct and simultaneous determination of four major furan derivatives in coffee grounds and brews
Authors: Albouchi Abdullatif , Murkovic Michael 
Published: February 2019

AbstractThe simultaneous quantification of two potential genotoxic hydroxymethyl furan derivatives in coffee (furfuryl alcohol and 5‐hydroxymethylfurfural) alongside their carboxylic acid derivatives (2‐furoic acid and 5‐hydroxymethyl furoic acid, respectively) was carried out. Their extraction from ground roasted coffee using sonication, simple shaking or heat‐assisted shaking lead to similar results. A minimum of 97.3% of the four furan derivatives were extracted during the first extraction cycle using water, whereas methanol showed considerably lower extraction efficiency. A simple high‐performance liquid chromatography method coupled with diode array detection was developed for the simultaneous determination and was applied to roasted coffee extracts or brews. No sample pre‐treatment except for centrifugation was needed. The diode array detector was used to assess the purity of the peaks of interest in analyzed samples against authentic standards. The linearity according to Mandel, accuracy (recovery ≥ 89.9%) and precision (inter‐ and intraday relative standard deviation ≤ 4.5%) were checked. The values for the limit of detection and quantification ranged within 0.11–0.76 and 0.35–2.55 μg/mL, respectively. Filtered and espresso brews were analyzed for the four furan derivatives where furfuryl alcohol showed double the concentration of 5‐hydroxymethylfurfural and about ten times the concentrations of 2‐furoic acid or 5‐hydroxymethyl furoic acid.
Recovery of species richness lags behind functional recovery in restored grasslands
Authors: Tölgyesi Csaba , Török Péter , Kun Róbert , Csathó András István , Bátori Zoltán , Erdős László , Vadász Csaba 
Published: February 2019

AbstractRestoration of grasslands on abandoned croplands is a major opportunity for land development in degraded landscapes. In this study, we compared the success of three restoration measures, spontaneous recovery, perennial‐crop–mediated restoration, and sowing native grasses, by using a unique combination of species‐based and trait‐based approaches. We sampled 373 4‐m2 plots in nearly 1,000 ha of 20‐year‐old–restored grasslands and 72 4‐m2 plots in three neighbouring ancient grasslands. Species richness and target species cover were higher in spontaneously recovered sites than in other restored sites, but reference sites had higher scores than all restored sites. Spontaneous sites had higher functional diversity than the other restored sites. Spontaneous and reference sites showed little difference in their functional diversity and in the community‐weighted means of relevant traits. Our findings indicate that the establishment limitation effect of the initial application of perennial crops or sowing competitive grasses can last for decades, hindering the recolonization of target species and the recovery of functional diversity; thus, they should be applied in landscapes with high cover of natural habitats only when inhibiting undesirable species is of paramount importance. The similar levels of functional niche saturation in spontaneous and reference sites may also indicate a self‐developed establishment‐limited situation after 20 years of succession. In combination with this, the lagging recovery of species richness entails lower functional redundancy in spontaneous sites. Our results underline the necessity of postrestorative management methods that reduce establishment limitation and contribute to the recolonization and subsequent coexistence of functionally similar species.