bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic Adaptability and Nutrient Scavenging in Toxoplasma gondii: Insights from Ingestion Pathway-Deficient Mutants
Authors: Rimple Patrick A. , Olafsson Einar B. , Markus Benedikt M. , Wang Fengrong , Augusto Leonardo , Lourido Sebastian , Carruthers Vern B. 
Published: November 2024

AbstractThe obligate intracellular parasiteToxoplasma gondiireplicates within a specialized compartment called the parasitophorous vacuole (PV). Recent work showed that despite living within a PV,Toxoplasmaendocytoses proteins from the cytosol of infected host cells via a so-called ingestion pathway. The ingestion pathway is initiated by dense granule protein GRA14, which binds host ESCRT machinery to bud vesicles into the lumen of the PV. The protein-containing vesicles are internalized by the parasite and trafficked to the Plant Vacuole-like compartment (PLVAC), where cathepsin protease L (CPL) degrades the cargo and the chloroquine resistance transporter (CRT) exports the resulting peptides and amino acids to the parasite cytosol. However, although the ingestion pathway was proposed to be a conduit for nutrients, there is limited evidence for this hypothesis. We reasoned that ifToxoplasmauses the ingestion pathway to acquire nutrients, then parasites lacking GRA14, CPL, or CRT should rely more on biosynthetic pathways or alternative scavenging pathways. To explore this, we conducted a genome-wide CRISPR screen in wild-type (WT) parasites and Δgra14, Δcpl, and Δcrtmutants to identify genes that become more fitness conferring in ingestion-deficient parasites. Our screen revealed a significant overlap of genes that become more fitness conferring in the ingestion mutants compared to WT. Pathway analysis indicated that Δcpland Δcrtmutants relied more on pyrimidine biosynthesis, fatty acid biosynthesis, TCA cycle, and lysine degradation. Bulk metabolomic analysis showed reduced levels of glycolytic intermediates and amino acids in the ingestion mutants compared to WT, highlighting the pathway’s potential role in host resource scavenging. Interestingly, ingestion mutants showed an exacerbated growth defect when grown in amino acid-depleted media, suggesting a role for theToxoplasmaingestion pathway during nutrient scarcity.ImportanceToxoplasma gondiiis an obligate intracellular pathogen that infects virtually any nucleated cell in most warm-blooded animals. Infections are asymptomatic in most cases but people with weakened immunity can experience severe disease. For the parasite to replicate within the host, it must efficiently acquire essential nutrients, especially as it is unable to make several key metabolites. Understanding the mechanisms by whichToxoplasmascavenges nutrients from the host is crucial for identifying potential therapeutic targets. Our study highlights the function of the ingestion pathway in sustaining parasite metabolites and contributes to parasite replication under amino acid limiting conditions. This work advances our understanding of the metabolic adaptability ofToxoplasma.
Cooperative Role of PACT and ADAR1 in Preventing Aberrant PKR Activation by Self-Derived Double-Stranded RNA
Authors: Manjunath Lavanya , Santiago Gisselle , Ortega Pedro , Sanchez Ambrocio , Oh Sunwoo , Garcia Alexander , Bournique Elodie , Bouin Alexis , Semler Bert L. , Setiaputra Dheva , Buisson Rémi 
Published: November 2024

ABSTRACTDouble-stranded RNAs (dsRNAs) produced during viral infections are recognized by the innate immune sensor protein kinase R (PKR), triggering a host translation shutoff that inhibits viral replication and propagation. Given the harmful effects of uncontrolled PKR activation, cells must tightly regulate PKR to ensure that its activation occurs only in response to viral infections, not endogenous dsRNAs. Here, we use CRISPR-Translate, a FACS-based genome-wide CRISPR-Cas9 knockout screening method that exploits translation levels as a readout and identifies PACT as a key inhibitor of PKR during viral infection. We find that cells deficient for PACT hyperactivate PKR in response to several different RNA viruses, raising the question of why cells need to limit PKR activity. Our results demonstrate that PACT cooperates with ADAR1 to suppress PKR activation from self-dsRNAs in uninfected cells. The simultaneous deletion of PACT and ADAR1 results in synthetic lethality, which can be fully rescued in PKR-deficient cells. We propose that both PACT and ADAR1 act as essential barriers against PKR, creating a threshold of tolerable levels to endogenous dsRNA in cells without activating PKR-mediated translation shutdown and cell death.
Cycles in Seizure Duration and Their Underlying Dynamics in the Tetanus Toxin Rat Model
Authors: Eskikand Parvin Zarei , Kazemi Sepehr , Cook Mark J. , Burkitt Anthony N. , Grayden David B. 
Published: November 2024

AbstractSeizure duration, a characteristic of epilepsy that is understudied in relation to its relationship with rhythmic cycles, provides critical insights into the severity and temporal dynamics of seizures. This study investigates the rhythmic patterns of seizure duration in the Tetanus Toxin (TT) rat model of epilepsy. Our analysis shows significant cyclical patterns in seizure durations, with periods ranging from 4 to 8 days across rats. The synchronization index and circular-linear correlations revealed phase-locked relationships between seizure durations and cycles, suggesting non-random, predictable temporal dynamics. Further analyses examined the relationship between seizure durations, inter-seizure intervals, and dominant EEG power. The findings highlight that seizure durations exhibit predictable rhythms, which could transform seizure prediction and enable time-based intervention strategies, ultimately improving epilepsy management and patient outcomes. These insights lay the groundwork for personalized, rhythm-aware therapeutic approaches.
Assessing the feasibility of a new approach to measure the full spectrum of CSF dynamics within the human brain using MRI: insights from a simulation study
Authors: van der Voort E.C. , van der Plas M.C.E. , Zwanenburg J.J.M. 
Published: November 2024

AbstractCerebrospinal fluid (CSF) dynamics are essential in waste clearance of the brain. Disruptions in CSF flow are linked to various neurological conditions, highlighting the need for accurate measurement of its dynamics. Current methods typically capture limited aspects of CSF movement or focus on a single anatomical region, presenting challenges for comprehensive analysis. This study proposes a novel approach using Displacement Encoding with Stimulated Echoes (DENSE) MRI to assess the full spectrum of CSF motion within the brain. Through simulations, we evaluated the feasibility of disentangling distinct CSF motion components, including heartbeat- and respiration-driven flows, as well as a net velocity component due to continuous CSF turnover, and tested the performance of our method under incorrect assumptions about the underlying model of CSF motion. Results demonstrate that DENSE MRI can accurately separate these components, and reliably estimated a net velocity, even when periodic physiological motions vary over time. The method proved to be robust for including low frequency components (LFO), incorrect assumption on the nature of the net velocity component and missing CSF components in the model. This approach offers a comprehensive measurement technique for quantifying CSF dynamics, advancing our understanding of the relative role of various drivers of CSF dynamics in brain clearance.
Lamin B1-dependent regulation of human separase in mitosis
Authors: Picotto Julien , Cipressa Francesca , Busso Didier , Cenci Giovanni , Bertrand Pascale , Pennarun Gaëlle 
Published: November 2024

ABSTRACTSeparase plays a central role in chromosome separation during mitosis and in centrosome cycle. Tight control of separase activity is required to prevent unscheduled resolution of sister chromatid cohesion and centrosome aberrations, thereby preserving genome stability. In mammals, despite their disassembly in early mitosis, some nuclear envelope components possess mitotic roles, but links with separase activity remain unexplored. Here, we uncover a new mechanism of separase regulation involving lamin B1, a key nuclear envelope factor. We show that separase and lamin B1 associate preferentially during early stages of mitosis. Importantly, lamin B1 depletion leads to an increase in separase recruitment on chromosomes together with premature chromatid separation, a phenotype reminiscent of separase overexpression. Conversely, similar to separase depletion, lamin B1 overexpression induces formation of diplochromosomes- resulting from chromatid separation failure-, in association with centrosome amplification. Importantly, increasing separase level prevents lamin B1-induced centrosome aberrations, suggesting a separase defect at their origin. Indeed, we show that overexpression of lamin B1 leads to a decrease in the recruitment of separase to the chromosome and a delay in its activity. Taken together, this study unveils a novel mechanism of separase regulation involving the nuclear envelope factor lamin B1, that is crucial for genome integrity maintenance.
Isoliquiritigenin attenuated cognitive impairment, cerebral tau phosphorylation and oxidative stress in a streptozotocin-induced mouse model of Alzheimer’s disease
Authors: Tang Zhi , Sha Ting , Wang Yuanshang , Xiao Yan , Ding Yuanting , Ni Ruiqing , Qi Xiaolan 
Published: November 2024

AbstractIntroductionTau hyperphosphorylation, mitochondrial dysfunction and oxidative stress play important roles in Alzheimer’s disease (AD). Isoliquiritigenin, a natural flavonoid isolated from the root of liquorice, has been shown to exert inhibitory effects on oxidative stress. Here, we assessed the neuroprotective effects of isoliquiritigenin on a streptozotocin-injected mouse model.MethodMolecular docking analysis performed for isoliquiritigenin with mTOR and ERK2. The mice (n = 27, male) were intracerebroventricularly injected with streptozotocin, treated with isoliquiritigenin (intraperitoneal, 2 days) and assessed using the Morris water maze. Oxidative stress, tau phosphorylation, mitochondrial dysfunction and synaptic impairment were evaluated in the cortex and hippocampal tissues of the mice by using biochemical assays and immunofluorescence staining.ResultsIsoliquiritigenin treatment mitigated the spatial memory capacity of streptozotocin-injected mice and alleviated tau phosphorylation at Ser396; the production of reactive oxygen species; the mitochondrial fission proteins Mfn1 and Mfn2; neuronal loss; and synaptic impairment (PSD95, SNAP25). Isoliquiritigenin treatment reduced the levels of mTOR Ser2448 and ERK1/2 T202/Y204 and upregulated the level of GSK-3βSer9 in the cortex and hippocampus of streptozotocin-injected mice.ConclusionIn conclusion, our findings suggest that isoliquiritigenin ameliorates streptozotocin-induced cognitive impairment, hyperphosphorylated tau, oxidative stress, mitochondrial dysfunction and synaptic impairment by decreasing mTOR and ERK activity and increasing GSK-3β activity.
What does your i-deal mean? Mediation of relative deprivation in the relationship between coworkers’ i-deals and employees’ voice behaviors
Authors: Li Kunlin , Sun Xin , Cheng Jin 
Published: November 2024

PurposeThis study examines the impact of perceptions of coworkers’ developmental i-deals on employees’ pro-organizational (POV) and self-interested voice (SIV) behaviors in high-tech companies, exploring the mediating role of perceived relative deprivation and the moderating role of psychological entitlement in these relationships.Design/methodology/approachThe study conducted a multi-wave, multi-source questionnaire survey among 409 employees in China. Statistical analyses were conducted using AMOS 22 and the PROCESS macro in SPSS 26.FindingsThe results suggest that perceptions of coworkers’ developmental i-deals decreased employees’ POV behaviors and increased SIV behaviors through focal employees’ perceived relative deprivation. In addition, employees’ psychological entitlement reinforced the effects of perceptions of coworkers’ developmental i-deals on employees’ perceived relative deprivation.Originality/valueUtilizing relative deprivation theory, this study discovered a new mediating mechanism (i.e. perceived relative deprivation) that links perceptions of coworkers’ developmental i-deals to focal employees’ POV behaviors and SIV behaviors. In addition, this study also revealed the moderating role of psychological entitlement in the relationship between perceptions of coworkers’ developmental i-deals and the focal employee’s relative deprivation.
Willingness to disclose information in mobile banking apps: how to tackle privacy concerns?
Authors: Luo Dan , Ni Xiewen , Aw Eugene Cheng-Xi , Tan Garry Wei-Han 
Published: November 2024

PurposeThis study aims to propose and validate a research framework pertaining to the willingness to disclose information in the context of mobile banking apps. The interrelationships between privacy protection, perceived personalization, social presence, design aesthetics, consumer empowerment, parasocial interactions and privacy concerns are assessed as antecedents of willingness to disclose information.Design/methodology/approachBased on a self-administered survey, this study gathered data from 450 Chinese consumers. The data were analyzed using structural equation modeling via SmartPLS 4 software.FindingsThe findings indicate that (1) privacy concerns are negatively related to willingness to disclose information, (2) parasocial interaction reduces privacy concerns, (3) consumer empowerment is positively influenced by privacy protection and perceived personalization and (4) social presence and design aesthetics positively contribute to the formation of parasocial interaction.Originality/valueThe current study serves to reinforce a theoretical understanding of the willingness to disclose information in mobile banking apps, which is underresearched. The findings offer alternative psychological mechanisms (i.e. consumer empowerment and parasocial interaction) and relevant mobile banking app attributes to explain the willingness to disclose information.
Does the fuzzy logic model explains more? Moderating effect of personality on the relationship between SR-HRM and job performance
Authors: Ozkan-Canbolat Ela , Kalemci R. Arzu , Kalemci-Tuzun Ipek 
Published: November 2024

PurposeThe purpose of this study is to examine the moderating effect of personality traits on social responsibility-oriented human resources management (SR-HRM) practices and job performance.Design/methodology/approachThe sample of the study consists of 442 employees working in firms developing knowledge and innovation-based systems in Ankara (Turkey). The strengths of this study stem from its methodology, which contributes to the literature in terms of analysis comparing linear and fuzzy set qualitative comparative analyzes (fsQCA).FindingsBoth linear analyses and fsQCA results show the moderating effect of conscientiousness on the relationship between job performance and SR-HRM. Both models demonstrate the impact of agreeableness on business performance. Study results indicate that linear and fsQCA examine the moderating effect of conscientiousness in the same way as the literature. The linear analysis results differ from fsQCA, which examined the moderating effect of compatibility in this study. The fsQCA results of this study show that SR-HRM affects job performance when extraversion and conscientiousness are taken into account.Research limitations/implicationsFurther research may compare linear and fuzzy logic models about moderating and mediating effects. Future researchers may highlight the increasing importance of linear and fsQCA in any organizational behavior or organizational theory subject.Practical implicationsThe main implication is that managers should consider the significant impacts of effective SR-HRM development on employees’ job performance. Personality traits should be taken into account in HRM processes such as recruitment and retention.Social implicationsThe main contribution of the current study is the insight it provides into the moderating effects of the big five personality traits on the relationship between job performance and SR-HRM and how they differ in the linear model and fsQCA.Originality/valueThe current study contributes to the relevant literature by presenting the research design and questions. Using a multi-method approach, this research provides a better understanding of patterns between variables and contributes to the existing literature.
The role of athlete brands for the international marketing of professional sports leagues: a case study of the German Bundesliga in Asia
Authors: Anderski Matthias , Stegmann Pascal , Dickson Geoff , Ströbel Tim 
Published: November 2024

PurposeThis study investigates the role of athlete brands for the international marketing of professional sports leagues by examining how athlete brands contribute to initiatives of football leagues.Design/methodology/approachThrough a single case study of the German Bundesliga underpinned by 17 semi-structured interviews with league, club and media executives, we identify specific brand co-creation opportunities of athlete brands and the sports league brand.FindingsOur findings reveal that athlete brands play a significant role in enhancing league visibility, engaging global audiences and unlocking new markets. Moreover, we identify specific opportunities for co-creating league brand meaning through strategic partnerships and athlete endorsements.Originality/valueThis research contributes to the literature by highlighting the importance of athlete brands in sports league marketing and offering insights for practitioners on innovative marketing strategies. Using a multi-actor perspective, this study provides valuable insights by revealing how sports leagues can pursue innovative marketing strategies by considering the role of athlete brands, thereby offering new ways for fans and sponsors to engage with the sports league.
Authentic leadership: Taylor’s version
Authors: Woods Kathryn , Kates Melissa Anne 
Published: November 2024

PurposeThe purpose of this manuscript is to explore an assignment given to students in an online survey of leadership theory and practice graduate course to help them learn and apply information on authentic leadership theory and critically examine the literature on this theory through a feminist lens.Design/methodology/approachAfter studying authentic leadership theory, students were prompted to examine Taylor Swift’s public statements, actions and impact on her industry and society and how her words and actions support or refute her status as an authentic leader. Students were challenged to examine how Swift’s gender impacts her status as an authentic leader and to critically examine how the demographics of the authors who developed this theory could affect its application.FindingsStudents critically engaged with the concept of authentic leadership, thoughtfully applied their knowledge to a real-world case study, and displayed an understanding of the interplay of gender and authentic leadership.Originality/valueRecommendations are provided to inspire new ideas for leadership educators who seek to prepare students to understand (the often gender-influenced) applications of authentic leadership in a modern setting.
Examining the complementary relationship between peer-to-peer lending and traditional banks in the USA
Authors: Cuadros Carrion Maria Belen , Czarnik Zofia Joanna , Lemke Robert J. , Tao Ran 
Published: November 2024

PurposeThe study aims to determine whether P2P lending serves as a complement or substitute to traditional bank lending, particularly in the context of decreasing bank branch availability. This research investigates how local banking competition, and demographic factors influence P2P loan activity. We aim to provide insight into the potential of P2P lending to enhance access to credit and present new evidence through 2023.Design/methodology/approachWe utilize a unique, comprehensive dataset that integrates data from the Census, financial institutions, and peer-to-peer (P2P) loans issued by Prosper, the first marketplace lending platform in the USA. This dataset covers over 5,000 US cities from 2018 to 2023. AI (ChatGPT) is employed to help merge approximately 166,000 P2P loans per year with the other datasets. Our analysis considers local banking market access and competition, demographics and P2P loan usage. We apply panel fixed-effect models to control for both city-specific and year-specific effects.FindingsWe find that access to financial institutions is positively related to P2P borrowing, with more Prosper loans issued in competitive banking markets and areas with a greater presence of small banks. P2P lending complements traditional bank lending, but it does not significantly benefit the lowest-income households or areas with high unemployment rates. P2P activity increases with income up to a median threshold of $75,000 and is not significantly affected by cities’ racial, educational and age compositions or population sizes. These findings suggest maintaining a competitive banking market that includes both traditional and P2P lending to promote broader credit access.Originality/valueThis research offers original insights by analyzing the interplay between traditional banking and peer-to-peer (P2P) lending. Prior research in this area is limited. Furthermore, we provide new evidence on the dynamics of this relationship through 2023. Utilizing a comprehensive dataset from Prosper, financial institutions and the Census, this study uniquely explores how local banking competition and demographic factors influence P2P loan activity. The study contributes to existing literature by highlighting that P2P lending complements, rather than substitutes for, traditional banking. Our findings underscore the importance of maintaining a competitive banking market that includes both traditional and Fintech lenders to enhance credit access.
Behavioural biases in sell-side equity analysts’ decision-making: a South African emerging market perspective
Authors: Sebastian Avani , Seetharam Yudhvir 
Published: November 2024

Purpose
Sell-side equity analysts are key information intermediaries, although prior literature has found that they exhibit behavioural biases. The aim of this study is to describe the process followed by analysts in formulating recommendations and to identify the behavioural biases that are likely to influence the process.

Design/methodology/approach
Semi-structured interviews were conducted with 20 sell-side equity analysts in an emerging market. This direct interaction allowed the researchers to gain insights into analysts’ use of information, related challenges and proclivity for biases.

Findings
The authors find evidence of intentional and spurious herding, availability, overconfidence and the disposition effect. In spite of the volume of information in corporate reports, a key source of new information is direct interaction with management, where analysts use their intuition or “gut feel”. Weary of the possibility of financial misstatement, they use these direct interactions to assess management’s trustworthiness. The authors find reputational motivations for the safety of herding around the broker consensus. Declining coverage of JSE-listed companies is therefore concerning for the analysts.

Originality/value
The study contributes to the limited qualitative research on analyst bias in the emerging South African economy. In addition, the focus on the information context of analysts juxtaposes corporate reporting and behavioural finance research to provide insights on the use of various sources of information for decision-making.