bluuebunny/crossref_metadata_2025_split huggingface dataset:
Satb2 regulates proliferation and nuclear integrity of pre-osteoblasts
Authors: Dowrey Todd , Schwager Evelyn E. , Duong Julieann , Merkuri Fjodor , Zarate Yuri A. , Fish Jennifer L. 
Published: March 2019

AbstractSpecial AT-rich sequence binding protein 2 (Satb2) is a matrix attachment region (MAR) binding protein. Satb2 impacts skeletal development by regulating gene transcription required for osteogenic differentiation. Although its role as a high-order transcription factor is well supported, other roles for Satb2 in skeletal development remain unclear. In particular, the impact of dosage sensitivity (heterozygous mutations) and variance on phenotypic severity is still not well understood. To further investigate molecular and cellular mechanisms of Satb2-mediated skeletal defects, we used the CRISPR/Cas9 system to generateSatb2mutations in MC3T3-E1 cells. Our data suggest that, in addition to its role in differentiation, Satb2 regulates progenitor proliferation. We also find that mutations inSatb2cause chromatin defects including nuclear blebbing and donut-shaped nuclei. These defects may contribute to a slight increase in apoptosis in mutant cells, but apoptosis is insufficient to explain the proliferation defects.Satb2expression exhibits population-level variation and is mostly highly expressed from late G1 to late G2. Based on these data, we hypothesize that Satb2 may regulate proliferation through two separate mechanisms. First, Satb2 may regulate the expression of genes necessary for cell cycle progression in pre-osteoblasts. Second, similar to other MAR-binding proteins, Satb2 may participate in DNA replication. Deficiencies in either of these processes could reduce the pace of cell cycle progression and contribute to nuclear damage. We also hypothesize that Satb2-mediated proliferation defects may be buffered in some genetic backgrounds, which provides some explanation for differences in severity of skeletal defects. Further elucidation of the role of Satb2 in proliferation has potential impacts on our understanding of both skeletal defects and cancer.
The bacterial endosymbiontWolbachiaincreases reproductive investment and accelerates the life cycle of ant colonies
Authors: Singh Rohini , Linksvayer Timothy A. 
Published: March 2019

AbstractWolbachiais a widespread group of maternally-transmitted endosymbiotic bacteria that often manipulates the reproductive strategy and life history of its solitary hosts to enhance its own transmission.Wolbachiaalso commonly infects eusocial insects such as ants, although the effects of infection on social organisms remain largely unknown. We tested the effects of infection on colony-level reproduction and life history traits in the invasive pharaoh ant,Monomorium pharaonis. First we compared the reproductive investment of infected and uninfected colonies with queens of three discrete ages, and we found that infected colonies had increased reproductive investment. Next, we compared the long-term growth and reproduction of infected and uninfected colonies across their life cycle, and we found that infected colonies had increased colony-level growth and early colony reproduction. These colony-level effects ofWolbachiainfection seem to result because of a ‘live fast, die young’ life history strategy of infected queens. Such accelerated colony life cycle is likely beneficial for both the host and the symbiont and may have contributed to success of the highly invasive pharaoh ant.
Transcriptome profiling of mouse samples using nanopore sequencing of cDNA and RNA molecules
Authors: Sessegolo Camille , Cruaud Corinne , Da Silva Corinne , Cologne Audric , Dubarry Marion , Derrien Thomas , Lacroix Vincent , Aury Jean-Marc 
Published: March 2019

AbstractOur vision of DNA transcription and splicing has changed dramatically with the introduction of short-read sequencing. These high-throughput sequencing technologies promised to unravel the complexity of any transcriptome. Generally gene expression levels are well-captured using these technologies, but there are still remaining caveats due to the limited read length and the fact that RNA molecules had to be reverse transcribed before sequencing. Oxford Nanopore Technologies has recently launched a portable sequencer which offers the possibility of sequencing long reads and most importantly RNA molecules. Here we generated a full mouse transcriptome from brain and liver using the Oxford Nanopore device. As a comparison, we sequenced RNA (RNA-Seq) and cDNA (cDNA-Seq) molecules using both long and short reads technologies and tested the TeloPrime preparation kit, dedicated to the enrichment of full-length transcripts. Using spike-in data, we confirmed that expression levels are efficiently captured by cDNA-Seq using short reads. More importantly, Oxford Nanopore RNA-Seq tends to be more efficient, while cDNA-Seq appears to be more biased. We further show that the cDNA library preparation of the Nanopore protocol induces read truncation for transcripts containing internal runs of T’s. This bias is marked for runs of at least 15 T’s, but is already detectable for runs of at least 9 T’s and therefore concerns more than 20% of expressed transcripts in mouse brain and liver. Finally, we outline that bioinformatics challenges remain ahead for quantifying at the transcript level, especially when reads are not full-length. Accurate quantification of repeat-associated genes such as processed pseudogenes also remains difficult, and we show that current mapping protocols which map reads to the genome largely over-estimate their expression, at the expense of their parent gene. The entire dataset is available from http://www.genoscope.cns.fr/externe/ONT_mouse_RNA.
Difference contact maps: from what to why in the analysis of the conformational flexibility of proteins
Authors: Iyer Mallika , Li Zhanwen , Jaroszewski Lukasz , Sedova Mayy , Godzik Adam 
Published: March 2019

AbstractProtein structures, usually visualized in various highly idealized forms focusing on the three-dimensional arrangements of secondary structure elements, can also be described as lists of interacting residues or atoms and visualized as two-dimensional distance or contact maps. We show that contact maps provide an ideal tool to describe and analyze differences between structures of proteins in different conformations. Expanding functionality of the PDBFlex server and database developed previously in our group, we describe how analysis of difference contact maps (DCMs) can be used to identify critical interactions stabilizing alternative protein conformations, recognize residues and positions controlling protein functions and build hypotheses as to molecular mechanisms of disease mutations.Author SummaryProtein folding is driven, and the three-dimensional structures of proteins are defined, by the non-local interactions between amino acid chains. However, the usual visualizations of protein structures focus on the overall chain topology and do not provide much information about the interactions defining them. Here we explore contact map visualization of protein structures, which directly focuses on interactions stabilizing the structure and thus can be easily used to understand effects of mutations on protein structure. We show that contact map visualization is particularly useful for comparing alternative conformations of proteins and identifying critical positions controlling functional flexibility.
Does regionalism matter? Local politics and foreign direct investment in South Korea
Authors: Kim Dong-Hun , Paek Sunwoo 
Published: March 2019

Purpose
The purpose of this paper is to examine how political/authoritarian regionalism affects foreign direct investment (FDI) in sub-national states in South Korea.

Design/methodology/approach
This paper employs statistical analysis to examine the relationship between regionalism and FDI, along with historical description of regionalism in South Korea.

Findings
The analyses suggest that not only authoritarian regionalism influence foreign investment to the region but also political regionalism matters. Sub-national states with higher authoritarian regionalism receive less foreign investment while sub-national states with high political regionalism, which imply political stability, receive more FDI than others.

Originality/value
The paper examined how local politics influence foreign investments in South Korea, and suggests stronger decentralization will positively influence FDI in the future.
Linking peace with reconciliation
Authors: Nam Kijeong 
Published: March 2019

Purpose
The purpose of this paper is to explain Japan’s role in the peace process on the Korean Peninsula that began in early 2018.

Design/methodology/approach
This paper emphasizes the historical context of international politics in Northeast Asia, rather than power politics or geopolitics. The paper reaffirms the significance of the ongoing peace process on the Korean Peninsula by considering a synthesis of three joint declarations published in 1998, 2000 and 2002 between the Republic of Korea (ROK) and Japan, the ROK and Democratic People’s Republic of Korea (DPRK), and between the DPRK and Japan.

Findings
The normalization of diplomatic relations between DPRK and Japan, along with reaffirmation of the joint declaration between the ROK and Japan, and the Panmunjeom Declaration, would be a base for denuclearizing Northeast Asia.

Originality/value
In Northeast Asia, historical reconciliation among the two Koreas and Japan and peace-building between the two parties on the Peninsula are closely linked. Moreover, the three bilateral relationships among these three parties are also the basis for creating a new multilateral security order in Northeast Asia.
Corporate dispersion and tax avoidance
Authors: Su Kun , Li Bin , Ma Chen 
Published: March 2019

PurposeThe purpose of this paper is to investigate the effects of corporate dispersion on tax avoidance from geographical and institutional dispersion perspectives by using evidence from China.Design/methodology/approachUsing a panel data of Chinese listed firms during 2003-2015, this paper estimates with correlation analysis and multiple regression analysis.FindingsBoth geographical and institutional dispersion are negatively associated with the degree of corporate tax avoidance. Furthermore, corporate governance mechanisms and female chief executive officers can mitigate the negative relation between corporate dispersion and tax avoidance. The results also indicate that ineffective internal control is one of the channels through which corporate dispersion reduces tax avoidance.Originality/valueThis is the first paper about the impact of firm dispersion on the degree of tax avoidance, complementing the research content of diversification and corporate decision-making.
A moderated mediation examination of Kahn’s theory in the development of new product performance
Authors: Lee Chih-Jen , Huang Stanley Y.B. 
Published: March 2019

PurposeThis study aims to propose a multilevel moderated mediation model of transformational leadership, corporate social responsible, organization-based self-esteem and job engagement to detect Kahn’s theory and predict new product development performance.Design/methodology/approachThis study used a longitudinal study over a six-month period to test the multilevel moderated mediation model. Empirical testing used a survey of 1,655 employees from 165 different R&D work group in Great China.FindingsTransformational leadership, corporate social responsible and organization-based self-esteem well predict employees’ job engagement and new product development performance and are moderated by open discussion of conflict.Originality/valueThis study is the first to propose a multilevel moderated mediation model to detect Kahn’s job engagement theory and predict new product development performance.
Multiple-relaxation-time lattice Boltzmann simulation for adsorption processes of carbon dioxide in a fixed-bed
Authors: Qiu ZhenYu , Ma Qiang , Zhang Ying , Yi Yiwu 
Published: March 2019

Purpose
This paper aims to discuss the dynamic adsorption processes of carbon dioxide in a porous fixed bed on the industrial scale, using a multiple-relaxation-time lattice Boltzmann (LB) model.

Design/methodology/approach
A multiple-relaxation-time LB model is developed to predict the dynamic adsorption processes of carbon dioxide in a porous fixed bed on the industrial scale. The breakthrough curves from the simulation results are compared with the experimental data to validate the reliability of this model, and the effects of flow velocity, porosity and linear driving force mass transfer coefficient on the adsorption behaviors of carbon dioxide are explored further.

Findings
The numerical results show that the improved fluid flux leads to the reduction in the time required for completion of adsorption processes nonlinearly, and the differential pressure significantly raises with the decreasing porosity of porous fixed bed for fixed values of Reynolds number and total adsorption capacity. The maximum adsorption ratio of carbon dioxide was found at Re = 12 in this work. In addition, the higher mass transfer resistance of adsorbent particles advances the appearance time of the breakthrough point and delays the completion time of the adsorption processes.

Originality/value
This work will provide a way to study the adsorption technology of carbon dioxide in the fixed-bed using the LB method.
An investigation of benchmark image collections: how different from digital libraries?
Authors: Qu Jingye , Chen Jiangping 
Published: March 2019

Purpose
This paper aims to introduce the construction methods, image organization, collection use and access of benchmark image collections to the digital library (DL) community. It aims to connect two distinct communities: the DL community and image processing researchers so that future image collections could be better constructed, organized and managed for both human and computer use.

Design/methodology/approach
Image collections are first identified through an extensive literature review of published journal articles and a web search. Then, a coding scheme focusing on image collections’ creation, organization, access and use is developed. Next, three major benchmark image collections are analysed based on the proposed coding scheme. Finally, the characteristics of benchmark image collections are summarized and compared to DLs.

Findings
Although most of the image collections in DLs are carefully curated and organized using various metadata schema based on an image’s external features to facilitate human use, the benchmark image collections created for promoting image processing algorithms are annotated on an image’s content to the pixel level, which makes each image collection a more fine-grained, organized database appropriate for developing automatic techniques on classification summarization, visualization and content-based retrieval.

Research limitations/implications
This paper overviews image collections by their application fields. The three most representative natural image collections in general areas are analysed in detail based on a homemade coding scheme, which could be further extended. Also, domain-specific image collections, such as medical image collections or collections for scientific purposes, are not covered.

Practical implications
This paper helps DLs with image collections to understand how benchmark image collections used by current image processing research are created, organized and managed. It informs multiple parties pertinent to image collections to collaborate on building, sustaining, enriching and providing access to image collections.

Originality/value
This paper is the first attempt to review and summarize benchmark image collections for DL managers and developers. The collection creation process and image organization used in these benchmark image collections open a new perspective to digital librarians for their future DL collection development.
Autonomy, health expenditures and development
Authors: Sommer Jamie 
Published: March 2019

PurposeState capacity for development is decomposed into two broad dimensions, intrinsic and extrinsic. The former concerns the state’s cohesiveness as a strategic actor, which encompasses the ability of the state to formulate and implement policy in a coherent fashion, and the latter is the state’s ability to extract and redistribute resources. Diverging from previous studies, this paper aims to consider how these intrinsic and extrinsic factors interact to increase health cross-nationally.Design/methodology/approachThis study provides a framework for understanding how domestic and international state autonomy in policy decisions improves the effectiveness of health spending at achieving its intended outcomes. Using two-way fixed effects regression models for a sample of 89 low and middle-income nations from 1996-2012, the author tests the claim that health expenditures reduce infant and child mortality more in nations with higher levels of international and domestic autonomy rather than those with lower levels.FindingsThe author finds evidence that supports the theoretical perspective that state autonomy in international and domestic policy, through cohesion, legitimacy and local insight, improves the effectiveness of health expenditures at reducing infant and child mortality.Research limitations/implicationsThe data only exist for 89 nations and from 1996 to 2012. In addition, the independent variables of interest that measure autonomy are not direct measures but instead indices.Originality/valueThe health and development of a nation’s citizenry largely depends on prioritizing expenditures in health. This research considers under what conditions nations can better prioritize health expenditures.
Lesson study outcomes: a theoretical model
Authors: Mynott John Paul 
Published: March 2019

Purpose
Lesson study (LS) research is disadvantaged by a lack of clarity surrounding the potential outcomes an LS cycle can produce for participant learning. The purpose of this paper is to set out a model of the potential outcomes an LS cycle can achieve. The model identifies the limitations that can occur in LS groups and how these limitations impact on the overall outcomes for participants.

Design/methodology/approach
Case studies are used to exemplify the different outcomes in the model taken from five years of LS work in a primary school in England. The case studies shape the four different outcomes of the model, defining and contextualising the attributes and characteristics of each outcome.

Findings
The model presented indicates that there are four key outcomes for LS cycles, with the most common outcome being a form of limited learning. The paper explores the limitations of time, collaboration and expertise to articulate how each of these limiting factors has a bearing on the overall outcome for an LS cycle.

Research limitations/implications
The model is currently based on a singular educational setting. This means that each outcome needs further exploration through wider LS work in order to clarify and refine the outcome model.

Practical implications
The outcome model will support the development of a shared vocabulary for discussing LS cycles. By articulating where on the outcome model an LS is, it is possible for researchers to discuss how to reduce the impact of limitations and other challenges to LS, enabling research to develop a more evaluation-led approach to using LS.

Originality/value
The outcome model supports LS researchers in articulating the outcomes of their LS cycles with a shared vocabulary. It addresses understudied areas of LS research, namely failed and dysfunctional LS cycles and identifies that while an LS can bring the potential for participant learning, the cycle outcomes are the starting point for participant change.
The impact of entertainment and social interaction with salespeople on mall shopper satisfaction
Authors: Elmashhara Maher Georges , Soares Ana Maria 
Published: March 2019

Purpose
The purpose of this paper is to understand the role played by emotional states in the relationship between entertainment and social interaction with salespeople and shopper satisfaction.

Design/methodology/approach
The proposed model was tested using a survey-based study, with a sample size of 318 mall shoppers.

Findings
The results indicate that pleasure and dominance are mediators in the relationship between entertainment and social interaction with salespeople and mall shopper satisfaction. Moreover, regarding the direct relations, entertainment directly influences satisfaction, while social interaction does not.

Practical implications
The results have practical implications for mall managers and mall developers. Specifically, entertainment can be used to attract shoppers and to enhance their satisfaction with the overall shopping experience. Practical suggestions to this end are offered.

Originality/value
This study’s contribution is twofold: first, this study adds to research by addressing the gap in research regarding shopper emotional states. Specifically, it addresses the mediating role played by emotional states of shoppers on the impact of entertainment and social interaction in satisfaction. Second, the study concentrates on the role of salespeople by focusing on the social aspects of the interaction.
Unfolding tensions within post-settlement governance and tribal economies in Aotearoa New Zealand
Authors: Mika Jason Paul , Smith Graham Hingangaroa , Gillies Annemarie , Wiremu Fiona 
Published: March 2019

PurposeThis paper aims to examine indigenous governance and economies of iwi Maori (Maori tribes) in Aotearoa New Zealand. Research into persisting inequities amongst iwi that have settled treaty claims and the potential for intervention through new governance models and indigenous entrepreneurship contextualise the paper.Design/methodology/approachKaupapa Maori (Maori philosophy) is used as an indigenous methodology to facilitate and empower transformative change, underpinned by Maori knowledge, language and culture. A multi-level approach is used to collect data from international, national and local tribal organisations. Validity is established through stakeholder engagement.FindingsA central challenge in the post-treaty settlement context is exponentialising tribal capabilities because of the multiple purposes ascribed to post-settled iwi. Four themes, characterised as “unfolding tensions”, offer a critique and basis for solving tribal development challenges: how do tribes create culturally grounded global citizens; how do tribes rebalance wealth creation and wealth distribution; how do tribes recalibrate tribal institutions; and how do tribes embed entrepreneurship and innovation within their economies?Research limitations/implicationsAs data collection is still underway, the paper is conceptual.Practical implicationsFive strategies to address unfolding tensions are identified for tribes to consider.Social implicationsTribal governors and tribal members are implicated in the analysis, as well as the architects of post-treaty settlement governance models.Originality/valueThe paper contributes to theorising about tribal governance, economies and entrepreneurship.