bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms involved in follistatin-induced hypertrophy and increased insulin action in skeletal muscle
Authors: Han X. , Møller L. L. V. , De Groote Estelle , Bojsen-Møller K.N. , Davey J. , Henríquez-Olguin C. , Li Z. , Knudsen J.R. , Jensen T. E. , Madsbad S. , Gregorevic P. , Richter E. A. , Sylow L. 
Published: March 2019

BackgroundSkeletal muscle wasting is often associated with insulin resistance. A major regulator of muscle mass is the transforming growth factor β (TGF-β) superfamily, including activin A, which causes atrophy. TGF-β superfamily ligands also negatively regulate insulin-sensitive proteins, but whether this pathway contributes to insulin action remains to be determined.MethodsTo elucidate if TGF-β superfamily ligands regulate insulin action we used an adeno-associated virus gene editing approach to overexpress the activin A inhibitor, follistatin (Fst288) in mouse muscle of lean and diet-induced obese mice. We determined basal and insulin-stimulated 2 deoxy-glucose uptake using isotopic tracers in vivo. Furthermore, to evaluate whether circulating Fst and activin A concentrations are associated with obesity, insulin resistance, and weight loss in humans we analysed serum from morbidly obese subjects before, 1 week, and 1 year after Roux-en-Y gastric bypass (RYGB).ResultsFst288 muscle overexpression markedly increased in vivo insulin-stimulated (but not basal) glucose uptake (+75%, p<0.05) and increased protein expression and intracellular insulin signalling of AKT, TBC1D4, PAK1, PDH-E1α, and p70S6K (p<0.05). No correlation was observed between the Fst288-driven hypertrophy and the increase in insulin-stimulated glucose uptake but Fst288 increased basal and insulin-stimulated protein synthesis. Importantly, Fst288 completely normalized muscle glucose uptake in insulin-resistant diet-induced obese mice. RYGB surgery doubled circulating Fst and reduced Activin A (−24%, p<0.05) concentration 1 week after surgery before any significant weight loss in morbidly obese normoglycemic patients, while major weight loss after 1 year did not further change the concentrations.ConclusionsWe here present evidence that Fst is a potent regulator of insulin action in muscle and in addition to AKT and p70S6K, we identify TBC1D1, TBC1D4 and PAK1 as Fst targets. A possible role for Fst in regulating glycemic control is suggested because circulating Fst more than doubled post RYGB surgery, a treatment that markedly improved insulin sensitivity. These findings demonstrate the therapeutic potential of inhibiting TGF-β superfamily ligands to improve insulin action and Fst’s relevance to muscle wasting associated insulin resistant conditions in mice and humans.
The Arabian camel,Camelus dromedariusinterferon epsilon: functional expression,in vitrorefolding, purification and cytotoxicity on breast cancer cell lines MDA-MB-231 and MCF-7
Authors: Abdel-Fattah Manal , Saeed Hesham , El-Shennawy Lamiaa , Shalaby Manal , Embaby Amira M. , Ataya Farid , E.Mahmoud Hoda , Hussein Ahmed 
Published: March 2019

AbstractThe current study highlights for the first time cloning, overexpression, purification, and assessing the cytotxcity of the novel interferon epsilon (IFNε), from the Arabian camelCamelus dromedarius, against two human breast cancer cell lines MDA-MB-231 and MCF-7. Full-length cDNA encoding interferon epsilon (IFNε) was isolated and cloned from the liver of the Arabian camel,C. dromedariususing reverse transcription-polymerase chain reaction. The sequence analysis of the camel IFNε cDNA showed a 582-bp open reading frame encoding a protein of 193 amino acids with an estimated molecular weight of 22.953 kDa. A BLAST search analysis revealed that theC. dromedariusIFNε shared high sequence identity with the IFN genes of other species, such asCamelus ferus,Vicugna pacos, andHomo sapiens. Expression of the camel IFNε cDNA inEscherichia coligave a fusion protein band of 22.73 kDa after induction with either isopropyl β-D-1-thiogalactopyranoside or lactose for 5 h. Recombinant IFNε protein was overexpressed in the form of inclusion bodies that were easily solubilized and refolded using SDS and KCl. The solubilized inclusion bodies were purified to apparent homogeneity using nickel affinity chromatography. We examined the effect of IFNε on two breast cancer cell lines MDA-MB-231 and MCF-7. In both cell lines, IFNε inhibited cell survival in a dose dependent manner as observed by MTT assay, morphological changes and apoptosis assay. Caspase-3 expression level was found to be increased in MDA-MB-231 treated cells as compared to untreated cells.
Niche partitioning and coexistence of two spiders of the genusPeucetia(Araneae, Oxyopidae) inhabitingTrichogoniopsis adenanthaplants (Asterales, Asteraceae): a population approach
Authors: Villanueva-Bonilla German Antonio , Safuan-Naide Suyen , Pires Mathias Mistretta , Vasconcellos-Neto João 
Published: March 2019

AbstractNiche theory suggests that the coexistence of ecologically similar species at the same site requires some form of resource partitioning that reduces or avoids interspecific competition. Here, we investigated the temporal and spatial niche differentiation of two sympatric congeneric spiders,Peucetia rubrolineataandP. flava, inhabitingTrichogoniopsis adenantha(Asteraceae) plants along an altitudinal gradient in various shaded and open areas in an Atlantic forest in Serra do Japi, SP, Brazil. In theory, the coexistence of twoPeucetiaspecies could be explained by: (1) temporal segregation; (2) differential use of the branches of the plant; (3) differential use of specific parts of the branches of the plant; (4) differential distribution in shaded and open areas; and (5) differential altitudinal distribution of the twoPeucetiaspecies. With respect to temporal niche, we observed that the two spider species had a similar age structure and similar fluctuation in abundance throughout the year. With respect to micro-habitat use, in both species, different instars used different plant parts, while the same instars of both species used the same type of substrate. However, the twoPeucetiaspecies segregated by meso-habitat type, withP. rubrolineatapreferringT. adenanthaplants in shaded areas andP. flavapreferring those in open areas. Our results support the hypothesis of niche partitioning begetting diversity, and highlight the importance of analysing habitat use at multiple scales to understand mechanisms related to coexistence.
An Integrated Chemical Proteomics Approach for Quantitative Profiling of Intracellular ADP-Ribosylation
Authors: Kalesh Karunakaran , Lukauskas Saulius , Borg Aaron J. , Snijders Ambrosius P. , Leung Anthony K. L. , Haskard Dorian O. , DiMaggio Peter A. 
Published: March 2019

AbstractADP-ribosylation is integral to a diverse range of cellular processes such as DNA repair, chromatin regulation and RNA processing. However, proteome-wide investigation of its cellular functions has been limited due to numerous technical challenges including the complexity of the poly(ADP-ribose) (PAR) chains, low abundance of the modification and lack of sensitive enrichment methods. To facilitate live-cell profiling of ADP-ribosylated proteins, we show that 2-alkyne-adenosine (2YnAd) is metabolically incorporated in mammalian cells and enables fluorescent detection and robust affinity enrichment of the modified proteins. We then present an integrated chemical biology approach that involves simultaneous metabolic incorporation of 2YnAd and the previously reported 6-alkyne-adenosine (6YnAd) in live cells followed by click chemistry with a capture reagent to facilitate highly sensitive and comprehensive enrichment of the modified proteins. By combining this dual metabolic labelling strategy with the tandem mass tag (TMT) isobaric mass spectrometry, we have quantified the responses of thousands of endogenous proteins to clinical PARP inhibitors Olaparib and Rucaparib. Our study provides insight into the wider scope of PARP targets and will help to advance further characterisation of their functional roles.
A new method of calculating interatomic spacing: the equal-ratio method
Authors: Fan Quncheng 
Published: March 2019

Based on a simple principle of analytical geometry, a new equal-ratio method has been developed to calculate the interatomic spacing of crystal structures. If an atom (x
2, y
2, z
2) or its equi-position atom (e + x
2, f + y
2, g + z
2) (e, f and g are integers) is located at the 1/r ≤ 1 of one interatomic spacing period d′[uvw] on the [uvw] atomic row passing through the atom (x
1, y
1, z
1), the distance between the two atoms can be calculated by the formula d
[uvw]
(1/r) = d′[uvw]/r, where d′[uvw] = (u
2
a
2 + v
2
b
2 + w
2
c
2 + 2uvabcosγ + 2vwbccosα + 2uwaccosβ)1/2 is the interlattice point spacing of the corresponding primary lattice of the crystal structure, 1/r is the interatomic spacing coefficient, and r is equal to the reciprocal of the common factor of (x
2 − x
1), (y
2 − y
1) and (z
2 − z
1). The reliability and advantages (no auxiliary view is required, suitable for arbitrary directions and for all crystal structures) of the equal-ratio method have been examined by calculations for the β-cristobalite SiO2 structure and Cu3Au I superstructure as well as face-centred cubic, body-centred cubic and hexagonal close-packed structures.
Social responsibility and event-sponsor portfolio fit
Authors: Close Scheinbaum Angeline , Lacey Russell , Drumwright Minnette 
Published: March 2019

Purpose
This study aims to examine the outcomes of consumer perceptions of event social responsibility (ESR) for a sponsored community event and its sponsor portfolio (i.e. group of sponsoring companies). It integrates a new antecedent and new moderators of ESR with extant findings to provide a comprehensive model that is theoretically grounded in social identity theory, congruency theory and image and affect transfer.

Design/methodology/approach
The authors test the theoretical framework via a field study of attendees (n = 879) at a sponsored, large-scale sporting event that provided ESR through health and wellness education and activities. A field study is especially appropriate because of the experiential nature of sponsored events and ESR.

Findings
Fan identification with the sport is an antecedent of ESR, and motivation to attend the event’s supporting activities moderates the relationship between fan identification and ESR. High event-sponsor fit strengthens the relationship between ESR and word-of-mouth and between ESR and sponsor patronage.

Research limitations/implications
This study illuminates the role of ESR as a key driver of outcomes for events and for their sponsor portfolio. Future research should investigate ESR in contexts other than sport and use longitudinal data that include actual purchases. It should further examine the construct of sponsor portfolio because so many events have multiple sponsors

Practical implications
Event sponsorship offers an attractive platform for brands to demonstrate good corporate citizenship; therefore, marketers should consider ESR as a key criterion when selecting events to sponsor. Marketers should sponsor events with high event-sponsor portfolio fit to enhance the outcomes related to ESR for both sponsors and events. This research generally underscores the importance of creating auxiliary, interactive experiences for event attendees.

Social implications
ESR entails that events should contribute or give back to the local communities and organizations in a charitable way to both help give back socially and to maximize success as measured by electronic word-of-mouth (eWOM) and sponsor patronage toward brands sponsoring the event.

Originality/value
This research identifies a new antecedent and new moderators of ESR and integrates them with extant findings to create a comprehensive, theoretically grounded model. It investigates outcomes for both the event and its sponsor portfolio, in contrast to the scholarship that tends to focus on the (title) sponsor.
Interpersonal practice in project marketing: how institutional logics condition and change them
Authors: Cova Bernard , Skålén Per , Pace Stefano 
Published: March 2019

Purpose
Project marketing is the specific activity of companies selling projects-to-order. Interpersonal practice is known to be important in this type of marketing. While this interpersonal practice has been little studied, some previous research suggests that changes in the institutional macro environment have affected it. Therefore, the purpose of this paper is to study today’s interpersonal practice in project business and how the institutional environment conditions it.

Design/methodology/approach
Semi-structured interviews were conducted with marketing managers at project-based firms in different business sectors in France and Sweden. Data collection and analysis was informed by grounded theory.

Findings
The paper identifies three types of interpersonal practice in project marketing, referred to as the transactional, the work-based and the socializing. Changes in these are explained in relation to the three institutional logics identified in the data: the market institutional logic of business ethics, the corporate institutional logic of rationalization and the family institutional logic of gender equality.

Research limitations/implications
Future studies can continue and broaden this work as it regards how the institutional conditioning of interpersonal practice varies with context.

Practical implications
By clearly categorizing the three types of interpersonal practice and their relative role today, companies can orient the activities of salespeople, business developers and other project marketers.

Social implications
The paper highlights how business ethics and gender equality have changed interpersonal practices in project marketing.

Originality/value
The paper contributes to the current debate on project marketing by identifying three types of interpersonal practice and by illustrating how institutional logics condition and change these. The paper shows that extra-business activities are needed less than previous research has argued with regard to maintaining customer relationships in-between projects.
NIE-based SME brand building in foreign markets: an exploratory study
Authors: Chung Jae-Eun , Jin Byoungho , Jeong So Won , Yang Heesoon 
Published: March 2019

Purpose
The purpose of this study is to examine the branding strategies of SMEs from NIEs, juxtaposing the different strategies used to specifically target developed and developing countries with regard to brand-building approach, type and number of brands and degree of standardization.

Design/methodology/approach
A case-study approach is used. In-depth interviews are conducted with 10 Korean consumer-goods SMEs exporting their own in-house brands.

Findings
Clear differences emerge between the strategies of SMEs entering developed countries and those entering developing countries, particularly regarding brand identity development, use of foreign sales subsidiaries and number and types of brands used. The authors find an interaction effect between product characteristics and host market levels of economic development, both of which influenced the degree of product standardization.

Originality/value
This study is the first attempt to uncover the branding strategies of NIE consumer-goods SMEs. The findings contribute to the field by extending our understanding of branding strategies used by consumer-goods SMEs from NIEs, thereby providing useful insight for other NIE enterprises when establishing branding strategies aimed at foreign markets.
A QoS-based approach for cloud-service matchmaking, selection and composition using the Semantic Web
Authors: Modi Kirit J. , Garg Sanjay 
Published: March 2019

PurposeCloud computing provides a dynamic, heterogeneous and elastic environment by offering accessible ‘cloud services’ to end-users. The tasks involved in making cloud services available, such as matchmaking, selection and composition, are essential and closely related to each other. Integration of these tasks is critical for optimal composition and performance of the cloud service platform. More efficient solutions could be developed by considering cloud service tasks collectively, but the research and academic community have so far only considered these tasks individually. The purpose of this paper is to propose an integrated QoS-based approach for cloud service matchmaking, selection and composition using the Semantic Web.Design/methodology/approachIn this paper, the authors propose a new approach using the Semantic Web and quality of service (QoS) model to perform cloud service matchmaking, selection and composition, to fulfil the requirements of an end user. In the Semantic Web, the authors develop cloud ontologies to provide semantic descriptions to the service provider and requester, so as to automate the cloud service tasks. This paper considers QoS parameters, such as availability, throughput, response time and cost, for quality assurance and enhanced user satisfaction.FindingsThis paper focus on the development of an integrated framework and approach for cloud service life cycle phases, such as discovery, selection and composition using QoS, to enhance user satisfaction and the Semantic Web, to achieve automation. To evaluate performance and usefulness, this paper uses a scenario based on a Healthcare Decision-Making System (HDMS). Results derived through the experiment prove that the proposed prototype performs well for the defined set of cloud-services tasks.Originality/valueAs a novel concept, our proposed integrated framework and approach for cloud service matchmaking, selection and composition based on the Semantic Web and QoS characterisitcs (availability, response time, throughput and cost), as part of the service level agreement (SLA) will help the end user to match, select and filter cloud services and integrate cloud-service providers into a multi-cloud environment.
Re‐visiting safe uncertainty: six perspectives for clinical practice and the assessment of risk
Authors: Mason Barry 
Published: March 2019

AbstractThis clinical paper brings together five of the author’s ideas since the original paper introducing the notion of safe uncertainty was published. Together with Cecchin’s (1987) use of curiosity these ideas are: a commitment to experimenting with difference; the taking of relational risks; a question related to family of origin scripts in coping with adversity; authoritative doubt; and the development of a culture of contribution. Clinical examples are given to aid practice and the assessment of risk.
Diagnostic dissonance and negotiations of biomedicalisation: mental health practitioners’ resistance to theDSMtechnology and diagnostic standardisation
Authors: Nelson Amber D. 
Published: March 2019

AbstractPrevious research demonstrates that biomedicalisation and diagnostic processes are intertwined in American mental health care, but few studies examine practitioners’ negotiations. This study examines how Mental Health Practitioners (MHPs) negotiate the Diagnostic and Statistical Manual (DSM), diagnosis, standardisation and biomedicalisation‐in‐practice. Feminist grounded theory analysis of 42 semi‐structured interviews with licensed adolescentMHPs reveals accounts of discursive, everyday resistance to theDSMtechnology and standardisation, which I regard as key aspects of biomedicalisation. Findings demonstrateMHPs seemingly practice what I term diagnostic dissonance: a deep conflict between their professional theoretical orientations and the biomedical model legitimated in theDSMtechnology and insurers’ diagnostic standardisation.MHPs enact dissonance by undermining theDSM, working around standardisation and by coding the social. Coding the social refers to the employment of V‐codes – illegitimate secondary diagnoses – which convey social and relational conditions of mental distress.MHPs’ contestations of theDSMand standardisation are responses to a healthcare infrastructure that decontextualises mental health. Practitioner resistance to biomedicalisation‐in‐diagnosis is important because the biomedicalisation of mental health takes focus away from the social and relational conditions and solutions to individual and community health and illness.
On the body of the consumer: performance‐seeking with wearables and health and fitness apps
Authors: Hardey Mariann (Maz) 
Published: March 2019

AbstractContributing to critical digital health research and the sociology of health consumption, this study investigates the phenomenon of self‐tracking and interpretation of consumer data via wearable technology and mobile fitness software applications (apps). It critically analyses qualitative data collected from members of running communities in the UK who are heavy users of apps and wearables. The study seeks to understand the meaning and practise of long‐term use of apps and wearables targeted at consumers interested in tracking fitness, and the collection of personal health information over time. The paper offers an interpretative perspective on runners as performance‐seeking fitness consumers engaged in long‐term self‐management of health. These consumers are driven by a profound motivation to visualise and embody a long‐term state of fitness. Participants were also hyper‐aware of advertising and promotional methods used to engage consumers. The findings raise concerns about the validity of personal fitness data, and how its collection promises improved personal health while visually promoting sought‐after fit bodies. Further research is required to understand the transformative impact of fitness‐tracking and how individuals negotiate personal classifications of health.
Justice, Legitimacy and the Authority of Legislation within the European Union
Authors: van den Brink Martijn 
Published: March 2019

AbstractWhat are we to make of the authority of legislation within the EU? EU lawyers have questioned the significance of legislative decision‐making within the EU. This article challenges these views and argues that the EU legislature must enjoy adequate freedom to shape EU law with the general interest in mind. Institutional accounts that seek to curtail the authority of legislation tend to rest upon ‘content‐dependent’ conceptions of political legitimacy, according to which the legitimacy of a decision depends on its moral qualities. Such conceptions overlook reasonable disagreements on justice and rest upon an overly optimistic (pessimistic) view of the Court (the legislature). The article argues for a content‐independent conception of legitimacy, following which the benefits of legislative decision‐making are more easily understood. The authority of legislation deserves wider recognition among EU lawyers for reasons of political legitimacy and because the EU legislature is better positioned to decide in the general interest.
So Long, Farewell, Auf Wiedersehen, Adieu: Brexit and the Charter of Fundamental Rights
Authors: Barnard Catherine 
Published: March 2019

The UK's relationship with the Charter of Fundamental Rights of the European Union can at best be described as strained, at worst, actively hostile. The Charter was, for the UK, an unwanted child, unloved at birth, grudgingly tolerated during life, and willingly surrendered at the death of the UK's membership of the EU. This article charts the UK's approach to the Charter from its inception to its demise in the EU (Withdrawal) Act 2018. It considers, in particular, the UK's so‐called opt out from the Charter in Protocol 30 and the confusion that has been generated as a result. It then argues that the Charter will have a legacy effect in the UK, primarily through the renaissance of the general principles of law.