bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human beta defensin 3 alters matrix metalloproteinase production in human dendritic cells exposed to Porphyromonas gingivalis hemagglutinin B
Authors: Raina Monica , Bates Amber M. , Fischer Carol L. , Progulske‐Fox Ann , Abbasi Taher , Vali Shireen , Brogden Kim A. 
Published: February 2018

AbstractBackgroundMatrix metalloproteinases (MMPs) are zinc‐ or calcium‐dependent proteinases involved in normal maintenance of extracellular matrix. When elevated, they contribute to the tissue destruction seen in periodontal disease. Recently, we found that human beta defensin 3 (HBD3), a cationic antimicrobial peptide, alters chemokine and proinflammatory cytokine responses in human myeloid dendritic cells exposed to Porphyromonas gingivalis hemagglutinin B (HagB). In this study, the hypotheses that HagB induces MMP production in dendritic cells and that HBD3 mixed with HagB prior to treatment alters HagB‐induced MMP profiles were tested.MethodsDendritic cells were exposed to 0.2 μM HagB alone and HagB + HBD3 (0.2 or 2.0 μM) mixtures. After 16 hours, concentrations of MMPs in cell culture media were determined with commercial multiplex fluorescent bead‐based immunoassays. An integrated cell network was used to identify potential HagB‐induced signaling pathways in dendritic cells leading to the production of MMPs.Results0.2 μM HagB induced MMP1, ‐2, ‐7, ‐9, and ‐12 responses in dendritic cells. 0.2 μM HBD3 enhanced the HagB‐induced MMP7 response (P < 0.05) and 2.0 μM HBD3 attenuated HagB‐induced MMP1, ‐7, and ‐9 responses (P < 0.05). The MMP12 response was not affected. In the predicted network, MMPs are produced via activation of multiple pathways. Signals converge to activate numerous transcription factors, which transcribe different MMPs.ConclusionHagB was an MMP stimulus and HBD3 was found to decrease HagB‐induced MMP1, ‐7, and ‐9 responses in dendritic cells at 16 hours, an observation that suggests HBD3 can alter microbial antigen‐induced production of MMPs.
Exposure to occupational noise and periodontitis in Korean workers
Authors: Shim Su‐Hyun , Han Dong‐Hun 
Published: February 2018

AbstractBackgroundChronic exposure to occupational noise is reported to cause a wide range of health problems. But, there are no reports on studies investigating the association between noise exposure and periodontitis. The purpose of this study was to examine the relationship between occupational noise exposure and periodontitis in a nationally representative sample of Korean workers.MethodsThis cross‐sectional study used data from the 4th Korean National Health and Nutrition Examination Survey. The study sample included 8,927 workers aged 19 to 54 years. Noise exposure assessment was performed by using self‐report questionnaires. The dependent variable was periodontitis. Age, sex, perceived stress level, place of residence, income, education, health behaviors (frequency of daily toothbrushing, binge drinking, smoking status, regular dental checkup), diabetes, obesity, and white blood cell count were included as covariates. Logistic regression analyses estimated the association between noise exposure and periodontitis after sequential adjustment.ResultsCompared with never‐exposed individuals, participants exposed to occupational noise had an increased prevalence of periodontitis. The association remained significant after full adjustment of covariates (odds ratio [OR] 1.34; 95% confidence interval [CI] 1.06 to 1.70). Association of noise exposure with periodontitis was particularly strong for individuals aged < 45 years (OR 1.65; 95% CI 1.22 to 2.23).ConclusionsOur study shows that there is an association between occupational noise exposure and periodontitis in a representative sample of Korean workers. Policy efforts to improve workplace noise environments might have an effect on improving periodontal health status among Korean workers.
Disintegration of the agricultural by‐product wheat bran under subcritical conditions
Authors: Reisinger Michael , Tirpanalan Özge , Pruksasri Suwattana , Kneifel Wolfgang , Novalin Senad 
Published: February 2018

AbstractBACKGROUNDThe disintegration of destarched wheat bran in water and sulfuric acid (pH 3) under subcritical conditions (275–300 °C) and at short reaction times (1–4 min) was investigated. A cascade process comprising a stepwise separation of the liquid was applied to reduce the formation of undesired degradation products.RESULTSThe highest degree of biomass disintegration (67% dry mass solubilization) was achieved by application of a cascade process at 275 °C (pH 3). Regarding the dissolution of carbohydrates (monomeric and oligomeric form), the total glucose yields remained below 60%, while the total xylose and arabinose yields were about 76% and 67%. Approximately 74% of the protein and 95% of the mineral fraction could be extracted. The application of the cascade process enabled a substantially reduced formation of degradation products.CONCLUSIONWhen operating hydrothermally and subcritically in order to avoid some problematic aspects of a biorefinery, an extensive disintegration and monomerization of wheat bran and its constituents remains difficult even under the tested conditions (300 °C, pH 3). However, the applied cascade process proved to be useful to increase the yields and to substantially reduce the formation of undesired degradation products. Despite this fact, increased water consumption has to be conceded. © 2018 Society of Chemical Industry
β‐Cyclodextrin‐modified three‐dimensional graphene oxide‐wrapped melamine foam for the solid‐phase extraction of flavonoids
Authors: Hou Xiudan , Lu Xiaofeng , Niu Panhong , Tang Sheng , Wang Licheng , Guo Yong 
Published: February 2018

AbstractA new three‐dimensional graphene oxide‐wrapped melamine foam was prepared and used as a solid‐phase extraction substrate. β‐Cyclodextrin was fabricated onto the surface of three‐dimensional graphene oxide‐wrapped melamine foam by a chemical covalent interaction. In view of a specific surface area and a large delocalized π electron system of graphene oxide, in combination with a hydrophobic interior cavity and a hydrophilic peripheral face of β‐cyclodextrin, the prepared extraction material was proposed for the determination of flavonoids. In order to demonstrate the extraction properties of the as‐prepared material, the adsorption energies were theoretically calculated based on periodic density functional theory. Static‐state and dynamic‐state binding experiments were also investigated, which revealed the monolayer coverage of flavonoids onto the β‐cyclodextrin/graphene oxide‐wrapped melamine foams through the chemical adsorption. 1H NMR spectroscopy indicated the formation of flavonoids–β‐cyclodextrin inclusion complexes. Under the optimum conditions, the proposed method exhibited acceptable linear ranges (2–200 μg/L for rutin and quercetin‐3‐O‐rhamnoside; 5–200 μg/L for quercetin) with correlation coefficients ranging from 0.9979 to 0.9994. The batch‐to‐batch reproducibility (n = 5) was 3.5–6.8%. Finally, the as‐established method was satisfactorily applied for the determination of flavonoids in Lycium barbarum (Goji) samples with relative recoveries in the range of 77.9–102.6%.
Keeping it authentic: Reconciling ORCID iDs gathered at submission with the author manuscript
Authors: Dunford Robin , Rosenblum Bruce 
Published: February 2018

Key points
Increasing pressure from funders to enable tracking of research outputs that result from their funding is driving the adoption of the ORCID iD.
Developers of submission and peer‐review systems have built tools to gather and authenticate ORCID iDs from authors during manuscript submission.
Crossref Auto‐update of author ORCID records requires authenticated iDs, but many publishers generate Crossref metadata XML from the author manuscript.
eXtyles ORCID Integration Suite allows automated reconciliation and synchronization between the submission system's transmittal file and the author manuscript.
Pseudo anti‐commuting Ricci tensor and Ricci soliton real hypersurfaces in complex hyperbolic two‐plane Grassmannians
Authors: Suh Young Jin , Kim Gyu Jong , Woo Changhwa 
Published: February 2018

AbstractIn this paper, first we introduce a new notion of pseudo anti commuting Ricci tensor for real hypersurfaces in complex hyperbolic two‐plane Grassmannians and prove a complete classification theorem that such a hypersurface must be a tube over a totally real totally geodesic , , a horosphere whose center at the infinity is singular or an exceptional case.
Nose resurfacing with free fasciocutaneous flaps in burns patients
Authors: Yen Cheng‐I , Yang Jui‐Yung , Chang Chun‐Shin , Huang Jung‐Ju , Wu Chih‐Wei , Chen Hung‐Chang , Yang Shih‐Yi , Chang Shu‐Yin , Chuang Shiow‐Shuh , Hsiao Yen‐Chang 
Published: February 2018

BackgroundNasal reconstruction after burn injury can be challenging due to limited availability of local flaps. We present our experience of free flap reconstruction for full‐thickness nasal defect after severe facial burn injury.MethodsBetween August 1998 and September 2015, six patients underwent nasal reconstruction with seven free flaps after burn injury. Among them, flame burn occurred in two patients, chemical burn in two, explosive burn in one, and contact thermal burn in one patient. The percentage of total body surface area ranged from 4% to 48%, and the face and forehead were involved in all patients. Their clinical and photographic records were retrospectively reviewed to evaluate the aesthetic results.ResultsFour ulnar forearm flaps, one radial forearm flap, one anterolateral thigh flap, and one medial sural artery perforator flap were used for nasal reconstruction. The nasal framework was constructed simultaneously using costal cartilage or conchal cartilage. The facial artery and vein were typically used as recipient vessels. One case each of partial necrosis and infection were noted during the average follow‐up of 59 months (range, 16–126 months). Patients had satisfactory aesthetic and functional outcomes after 4.5 times (range, 2–7 times) refinement operation.ConclusionsFree flap is an applicable alternative to restore nasal skin envelope, with rebuilding the nasal framework performed in the same stage after severe facial burn injury. Through thoughtful planning and sufficient refinement, satisfactory aesthetic, and functional results are achievable.
In vivo self‐gated 23Na MRI at 7 T using an oval‐shaped body resonator
Authors: Platt Tanja , Umathum Reiner , Fiedler Thomas M. , Nagel Armin M. , Bitz Andreas K. , Maier Florian , Bachert Peter , Ladd Mark E. , Wielpütz Mark O. , Kauczor Hans‐Ulrich , Behl Nicolas G.R. 
Published: February 2018

PurposeThis work faces three challenges of sodium (23Na) torso MRI on the way to quantitative 23Na MRI: Development of a 23Na radiofrequency transmit and receive coil covering a large part of the human body in width and length for 23Na MRI at 7 T; reduction of blurring due to respiration in free‐breathing 23Na MRI using a self‐gating approach; and reduction of image noise using a compressed‐sensing reconstruction.MethodsAn oval‐shaped birdcage resonator with a large field of view of (400 mm)3 and a homogeneous transmit and receive field distribution was designed, simulated, and implemented on a 7T MR system. In free‐breathing 3‐dimensional radial 23Na MRI (acquisition time ≈ 30 minutes), retrospective respiratory self‐gating was applied, which sorts the acquired projections into two respiratory states based on the intrinsic respiration‐dependent signal changes. Furthermore, a 3‐dimensional dictionary‐learning compressed‐sensing reconstruction was applied.ResultsThe developed body coil provided homogeneous radiofrequency excitation (flip angle error of 4.9% in central region of interest of 23 × 13 × 10 cm3) and homogeneous signal reception. The self‐gating approach allowed for separation of the full data set into two subsets associated with different respiratory states (inhaled and exhaled), and thereby reduced blurring due to respiration in the separated images. Image noise was markedly reduced by the compressed‐sensing algorithm.ConclusionThe presented body coil enables full body width 23Na MRI with long z‐axis coverage at 7 T for the first time. Additionally, the retrospective respiratory self‐gating performance is demonstrated for free‐breathing lung and abdominal 23Na MRI in 3 subjects.
Controlling T2 blurring in 3D RARE arterial spin labeling acquisition through optimal combination of variable flip angles and k‐space filtering
Authors: Zhao Li , Chang Ching‐Di , Alsop David C. 
Published: February 2018

PurposeTo improve the SNR efficiency and reduce the T2 blurring of 3D rapid acquisition with relaxation enhancement stack‐of‐spiral arterial spin labeling imaging by using variable refocusing flip angles and k‐space filtering.MethodsAn algorithm for determining the optimal combination of variable flip angles and filtering correction is proposed. The flip angles are designed using extended phase graph physical simulations in an analytical and global optimization framework, with an optional constraint on deposited power. Optimal designs for correcting to Hann and Fermi window functions were compared with conventional constant amplitude or variable flip angle only designs on 6 volunteers.ResultsWith the Fermi window correction, the proposed optimal designs provided 39.8 and 27.3% higher SNR (P < .05) than conventional constant amplitude and variable flip angle designs. Even when power deposition was limited to 50% of the constant amplitude design, the proposed method outperformed the SNR (P < .05) of these 2 conventional approaches by 32.5 and 20.7%. The sharpness and the contrast between gray and white matter were improved with the k‐space filtering correction for all of the flip angle designs. The improvements were moderate for the Hann window correction.ConclusionThis work demonstrates that variable flip angles can be derived as the output of an optimization problem. The combined design of variable flip angle and k‐space filtering provided superior SNR to designs primarily emphasizing either approach singly.
Minimum echo time PRESS‐based proton observed carbon edited (POCE) MRS in rat brain using simultaneous editing and localization pulses
Authors: Kumaragamage Chathura , Madularu Dan , Mathieu Axel P. , Lupinsky Derek , de Graaf Robin A. , Near Jamie 
Published: February 2018

PurposeIndirect 13C MRS by proton‐observed carbon editing (POCE) is a powerful method to study brain metabolism. The sensitivity of POCE‐MRS can be enhanced through the use of short TEs, which primarily minimizes homonuclear J‐evolution related losses; previous POCE‐MRS implementations use longer than optimal echo times due to sequence limitations, or short TE image selected in vivo spectroscopy‐based multi‐shot acquisitions for 3D localization. To that end, this paper presents a novel single‐shot point resolved spectroscopy (PRESS)‐localized POCE‐MRS sequence that involves the application of simultaneous editing and localization pulses (SEAL)‐PRESS, allowing the TE to be reduced to a theoretically optimal value of ∼ 1/JHC.MethodsThe optimized SEAL‐PRESS sequence was first evaluated in simulation and in phantom; next, the sequence was validated with dynamic in vivo POCE‐MRS performed in a rat preparation during a 1,6‐13C2‐Glc infusion, and on a microwave fixed rat brain following a 2‐hour [1,6‐13C2]‐Glc infusion. POCE spectra from the SEAL‐PRESS sequence were compared against a previously described 12.6‐ms PRESS‐POCE sequence utilizing a classical carbon editing scheme.ResultsThe SEAL‐PRESS sequence provides > 95% editing efficiency, optimal sensitivity, and localization for POCE MRS with an overall sequence TE of 8.1 ms. Signal amplitude of 13C‐labeled metabolites Glu‐H4, Gln‐H4, Glx‐H3, Glc‐H6 +Glx‐H2, and Asp‐H2 were shown to be improved by >17% relative to a 12.6‐ms PRESS‐POCE sequence in vivo.ConclusionWe report for the first time, a single‐shot PRESS‐localized and edited 8.1‐ms TE POCE‐MRS sequence with optimal sensitivity, editing efficiency, and localization.
Robust SENSE reconstruction of simultaneous multislice EPI with low‐rank enhanced coil sensitivity calibration and slice‐dependent 2D Nyquist ghost correction
Authors: Lyu Mengye , Barth Markus , Xie Victor B. , Liu Yilong , Ma Xin , Feng Yanqiu , Wu Ed X. 
Published: February 2018

PurposeTo improve simultaneous multislice (SMS) EPI by robust Nyquist ghost correction in both coil sensitivity calibration and SMS reconstruction.MethodsTo derive coil sensitivity and slice‐dependent phase difference map between positive‐ and negative‐echo images, single‐band EPI reference data are fully sampled with EPI parameters matched to SMS acquisition. First, the reference data are organized into positive‐ and negative‐echo virtual channels where missing data are estimated using low‐rank‐based simultaneous autocalibrating and k‐space estimation (SAKE) at small matrix size. The resulting ghost‐free positive‐ and negative‐echo images are combined to generate coil sensitivity maps. Second, full‐matrix positive‐ and negative‐echo images are SENSE reconstructed from the reference data. Their phase difference or error map is then calculated. Last, SMS EPI is reconstructed using phase error correction SENSE (PEC‐SENSE) that incorporates phase error map into coil sensitivity maps for negative‐echo data. The proposed method was evaluated using both experimental data from 7T systems and simulations.ResultsVirtual coil SAKE eliminated Nyquist ghosts in the single‐band EPI, yielding high‐quality coil sensitivity maps and phase error maps. The subsequent PEC‐SENSE robustly reconstructed SMS EPI under various conditions, including presence of in‐plane acceleration, with lesser artifacts and higher temporal SNR than slice‐dependent 1D linear correction method.ConclusionThe proposed procedure of virtual coil SAKE calibration and PEC‐SENSE reconstruction substantially reduces all ghost‐related artifacts originating either directly from SMS EPI data or indirectly from EPI‐based coil sensitivity maps. It is computationally efficient, and generally applicable to all SMS EPI‐based applications.
Cerebellar herniation demonstrated by the occipitum‐dens line: Ultrasonography assessment of normal fetuses, fetuses with myelomeningocele, and fetuses that underwent antenatal myelomeningocele surgery
Authors: Barreto Enoch Quinderé de Sá , Cavalheiro Sérgio , Milani Herbene José Figuinha , Barbosa Maurício Mendes , Araujo Júnior Edward , Nardozza Luciano Marcondes Machado , Moron Antonio Fernandes 
Published: February 2018

AbstractObjectiveTo establish a method to quantify the position of the cerebellum by ultrasonography in normal fetuses, fetuses with myelomeningocele (MMC), and fetuses that underwent in utero MMC repair.MethodsReference points identifiable on ultrasound were established. The basilar portion of the occipital bone and upper portion of the odontoid process were considered as the external and internal limits, respectively, of the level zero of a line that was designated the occipitum‐dens line (ODL). Eighty‐three normal fetuses were assessed 1 occasion (cross‐sectional study) in addition to 25 fetuses with MMC and 25 fetuses that underwent in utero MMC repair. The groups were compared using analysis of variance or the Kruskal‐Wallis test. The intraclass correlation coefficient (ICC) was used to determine intra‐ and interobserver reproducibility.ResultsThe analysis of ODL indicated that all normal fetuses had the cerebellar tonsil above level zero at a mean distance of 2.8 ± 1.1 mm (P = 0.125). The fetuses with MMC had the cerebellar tonsil below level zero, except in 2 cases of myelocystocele. All the fetuses that underwent in utero MMC repair exhibited regression of the cerebellar herniation (0.5 mm per week,P < 0.005). Herniation increased gradually (1.0 mm per week,P < 0.005) in fetuses that did not undergo MMC repair. ICC indicated good intra‐ and interobserver reproducibility (0.996 and 0.983, respectively).ConclusionThe reference points for ODL may be used to assess cerebellar height. ODL allowed the demonstration of the regression of cerebellar herniation in fetuses that underwent in utero MMC repair.
Preparation and application of polyaniline nanofibers: an overview
Authors: Wang Yanmin 
Published: February 2018

AbstractPolyaniline (PANI) nanofibers have enhanced water processibility and improved sensitivity and response time when exposed to chemical vapors, and unique advantages in other fields including electric devices and corrosion inhibition. Therefore, PANI nanofibers have attracted great interest and have potential for many commercial applications. In this paper, the advantages, formation mechanism, characteristics and factors influencing various methods for preparing PANI nanofibers are described in detail. Their conductive properties and mechanism are briefly introduced. Finally, the potential applications of PANI nanofibers are elaborated in many fields such as gas sensors, capacitors, electrode materials for cells, removal of contaminants from water, corrosion inhibition, enzyme immobilization, Schottky diodes, and so on. © 2018 Society of Chemical Industry
Illuminating structure and acyl donor sites of a physiological transglutaminase substrate from Streptomyces mobaraensis
Authors: Juettner Norbert E. , Schmelz Stefan , Bogen Jan P. , Happel Dominic , Fessner Wolf‐Dieter , Pfeifer Felicitas , Fuchsbauer Hans‐Lothar , Scrima Andrea 
Published: February 2018

AbstractTransglutaminase from Streptomyces mobaraensis (MTG) has become a powerful tool to covalently and highly specifically link functional amines to glutamine donor sites of therapeutic proteins. However, details regarding the mechanism of substrate recognition and interaction of the enzyme with proteinaceous substrates still remain mostly elusive. We have determined the crystal structure of the Streptomyces papain inhibitory protein (SPIp), a substrate of MTG, to study the influence of various substrate amino acids on positioning glutamine to the active site of MTG. SPIp exhibits a rigid, thermo‐resistant double‐psi‐beta‐barrel fold that is stabilized by two cysteine bridges. Incorporation of biotin cadaverine identified Gln‐6 as the only amine acceptor site on SPIp accessible for MTG. Substitution of Lys‐7 demonstrated that small and hydrophobic residues in close proximity to Gln‐6 favor MTG‐mediated modification and are likely to facilitate introduction of the substrate into the front vestibule of MTG. Moreover, exchange of various surface residues of SPIp for arginine and glutamate/aspartate outside the glutamine donor region influences the efficiency of modification by MTG. These results suggest the occurrence of charged contact areas between MTG and the acyl donor substrates beyond the front vestibule, and pave the way for protein engineering approaches to improve the properties of artificial MTG‐substrates used in biomedical applications.