bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experience of combined use of a Bakri uterine balloon and a non‐pneumatic anti‐shock garment in a university hospital in Colombia
Authors: Escobar María F. , Suso Juan P. , Hincapié María A. , Echavarría María P. , Fernández Paula , Carvajal Javier 
Published: May 2019

AbstractObjectiveTo compare the outcomes of women with postpartum hemorrhage (PPH) refractory to initial management and in a state of hypoperfusion between management with a non‐pneumatic anti‐shock garment (NASG) and Bakri balloon and management with other surgical interventions.MethodsA retrospective observational descriptive study of women with PPH and hemorrhagic shock who were treated at a high complexity obstetric unit in Columbia between 2011 and 2017. Clinical records were reviewed and women were divided in two groups by clinical management. Group 1 women were managed with surgical interventions; group 2 women were managed with NASG plus a Bakri balloon.ResultsOverall, 142 women were treated for PPH, with 69 in group 1 and 73 in group 2). There were differences between group 1 and group 2 in the degree of hypovolemic shock (shock index: 1.1 vs 0.9, P=0.02), indicators associated with hypoperfusion (lactic acid, 2.9 vs 1.9 mmol/L, P=0.001), and frequency of transfusion of blood components (68% vs 44%, P<0.05).ConclusionsThe joint use of NASG and Bakri balloon in PPH management seemed to improve hypoperfusion‐related markers such as lactic acid and shock index, and reduce the frequency of additional blood transfusion.
Effects of low maternal heart rate on fetal growth and birthweight
Authors: Odendaal Hein , Kieser Eduard , Nel Daan , Brink Lucy , du Plessis Carlie , Groenewald Coen , Lucchini Maristella , Fifer William P. , Myers Michael M. 
Published: May 2019

AbstractObjectiveTo evaluate the association between birthweight and maternal heart rate (MHR) or heart rate variability (HRV) under resting conditions at 20–24 gestational weeks and 34 weeks or later (34+ weeks).MethodsData were retrospectively reviewed from the Safe Passage Study, a prospective longitudinal cohort study of alcohol use in pregnancy and birth outcomes in Cape Town, South Africa, between August 2007 and January 2015. Using custom‐designed software, MHR and indicators of HRV were obtained from the recorded maternal electrocardiograms and compared with birthweight and z‐scores of birthweight adjusted for gestation and gender.ResultsData from 5655 women were included. MHR increased from 84.6 bpm at 20–24 weeks to 88.3 bpm at 34+ weeks. Increasing MHR from 70–80 to 80–90 and 90–100 bpm at 20–24 weeks was associated with increasing birthweight from 2940 to 2998 and 3032 g, respectively (P<0.05). MHR and HRV contributed 29% to the variability associated with birthweight, whereas maternal body mass index at recruitment contributed 44%. Similar associations were observed for MHR at 34+ weeks.ConclusionThe observed association of low maternal heart rate with birthweight might help to identify pregnancies at risk of poor fetal growth.
Exploring Educators' Figured Worlds of Controversial Literature and Adolescent Readers
Authors: Hartsfield Danielle E. , Kimmel Sue C. 
Published: May 2019

AbstractEducators assume the role of gatekeepers when they make literature selections for adolescent classrooms. Their taken‐for‐granted assumptions, or figured worlds, about adolescent and youth literature may inform their decisions to either select or preemptively censor books. In this qualitative study, the authors examined the figured worlds of six preservice and inservice middle and secondary educators as they read and discussed controversial youth literature (i.e., challenged or banned books). Three themes emerged in the analysis: figured worlds about youth, figured worlds about “appropriate” literature, and figured worlds about how adolescents should access controversial literature. These figured worlds may shape which books these participants would choose to include or exclude from their classrooms. The authors conclude by sharing suggestions that educators may use to deconstruct their own figured worlds and promote wider access to a diverse range of literature for adolescent readers.
Community pharmacist patient care services: A systematic review of approaches used for implementation and evaluation
Authors: Bacci Jennifer L. , Bigham Kyle A. , Dillon‐Sumner Laurel , Ferreri Stefanie , Frail Caitlin K. , Hamada Cory Y. , Lantaff Wendy , McGivney Melissa S. , Renner Hannah , Snyder Margie E. , Curran Geoffrey M. 
Published: May 2019

AbstractBackgroundStudies have identified the positive impact of community pharmacist patient care services. Identifying the most effective implementation strategies and performing robust research are critical to sustaining evidence‐based patient care in this setting. This systematic review aimed to describe: (1) implementation strategies, and (2) dissemination and implementation (D&I) outcomes used to evaluate the implementation of patient care services in community pharmacies.MethodsPubMed/MEDLINE, EMBASE, and International Pharmaceutical Abstracts were searched for articles that were: (1) written in English and published in 1985 or later, (2) described a peer‐reviewed empirical study or practice experience implementing a patient care service in a community pharmacy, and (3) described the strategies used to implement and/or evaluate the implementation of the service. Two investigators reviewed the abstract, and full‐text when necessary, of each article to assess eligibility. Data were extracted from each article by two investigators using a standardized form. Implementation strategies were categorized using the Expert Recommendations for Implementation Change (ERIC) study terminology. The D&I outcomes were classified using the Proctor and colleagues terminology. All data were summarized using descriptive statistics.ResultsTwo hundred and thirty‐seven articles were included, 223 of which described at least one implementation strategy and 224 described at least one D&I outcome. “Train and educate stakeholders” and “engage consumer” were the most common and “provide interactive assistance” and “adapt and tailor to context” were the least commonly reported implementation strategies. Reach, acceptability, and adoption were the most common and fidelity and sustainability were the least commonly evaluated D&I outcomes. Most studies used quasi‐experimental or observational designs.ConclusionsThis systematic review used published manuscripts to identify the breadth and depth of implementation and evaluation strategies being utilized in various community pharmacies. Findings suggest opportunities for further research and innovation via collaboration between pharmacists and implementation practitioners and researchers.
miRNA‐30‐3p improves myocardial ischemia via the PTEN/PI3K/AKT signaling pathway
Authors: Yin Yugang , Yang Chun 
Published: May 2019

AbstractThe aim of this study was to explain the effect and mechanisms of miRNA‐30‐3p in myocardial ischemia‐induced cell apoptosis in vitro and in vivo studies. In the cell experiment, the H9C2 cells were divided into the normal control (NC), and the model, miRNA, and miRNA + phosphatidylinositol 3‐kinase (PI3K) inhibitor groups. The cell survival rates of the different groups were measured with the 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium assay kit; the lactate dehydrogenase (LDH), malondialdehyde (MDA) content, and superoxidedimutase (SOD) activity in the bathing medium were assayed for the evaluation of myocardial cell injury. The cell apoptosis rate of different groups was measured with flow cytometry analysis. The relative protein expressions of different cell groups were evaluated by Western blot analysis. In the vivo study, the Sprague‐Dawley rats were divided into four groups: the NC group, the model group, miRNA group, and the (miRNA + PI3K inhibitor) group. The pathological observations, cell apoptosis, LDH, SOD, MDA, and relative protein expressions were evaluated with hematoxylin and eosin, enzyme‐linked immunosorbent assay, terminal deoxynucleotide transferase dUTP nick‐end labeling or immunohistochemical methods. The results show that miRNA‐30‐3p had the effect of improving cell apoptosis induced by myocardial ischemia in vitro and in vivo studies by the regulation of the PTEN/PI3K/AKT pathway.
Quercetin augments apoptosis of canine osteosarcoma cells by disrupting mitochondria membrane potential and regulating PKB and MAPK signal transduction
Authors: Ryu Soomin , Park Sunwoo , Lim Whasun , Song Gwonhwa 
Published: May 2019

AbstractOsteosarcoma is a mesenchymal malignant bone tumor accompanied by a high rate of lung metastasis and short survival in dogs. Although various therapies have been reported, the etiological mechanism of osteosarcoma remains undetermined and the development of novel therapeutic agents is warranted. In this study, we have reported the diverse functions of quercetin, one of the well‐known flavonoid, in D‐17 and DSN (canine osteosarcoma) cell lines. Current results indicate that quercetin decreases proliferative properties and increases programmed cell death, in addition to altering the cell cycle, mitochondrial depolarization, level of reactive oxygen species, and concentration of cytoplasmic calcium in both cells. Furthermore, it was observed that quercetin suppresses phosphorylation of AKT, P70S6K, and S6 proteins and upregulates phosphorylation of ERK1 or 2, P38, c‐Jun N‐terminal kinase, and P90RSK proteins in both cell lines. Collectively, we suggest that quercetin can be used as a pharmacological agent for suppressing the proliferation and inducing the apoptosis of canine osteosarcoma cells.
S100 A9 is expressed and secreted by the oviduct epithelium, interacts with gametes and affects parameters of human sperm capacitation in vitro
Authors: Massa Estefanía , Prez Gastón , Zumoffen Carlos , Morente Carlos , Ghersevich Sergio 
Published: May 2019

AbstractOur previous findings demonstrate that some oviductal secretion proteins bind to gametes and affect sperm physiology and gamete interaction. One of these proteins possesses an estimated molecular weight of 14 kDa. The objective of this study was to isolate and identify this 14 kDa protein, to localize it in the human oviduct, to detect gamete binding sites for the protein, and to evaluate its effects on sperm capacitation parameters and gamete interaction. Explants from the human oviductal tissues of premenopausal women were cultured in the presence of [35S]‐Methionine‐proteins ([35S]‐Met‐proteins). De novo synthesized secreted [35S]‐Met‐proteins were isolated from the culture media by affinity chromatography using their sperm membrane binding ability and analysed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Using liquid chromatography‐tandem mass spectrometry peptide sequencing, human S100 A9 was identified as one of the isolated proteins from the 14 kDa protein band. S100 A9 was detected in oviduct epithelium and oviduct secretion using immunohistochemistry and a Western blot. S100 A9 binding to human oocytes and spermatozoa was assessed by indirect immunofluorescence. The acrosome reaction (AR) affected S100 A9 ability to bind sperm cells. The presence of S100 A9 significantly increased both the induced AR and the sperm protein tyrosine phosphorylation, with respect to controls. However, the protein did not affect sperm‐zona pellucida interaction. Results indicate that S100 A9 is present in the human oviduct and that it modulates parameters of sperm capacitation in vitro. Hence, the protein might contribute to the regulation of the reproductive process in the oviductal microenvironment.
Inhibition of mitochondrial activity ameliorates atherosclerosis in ApoE−/− mice via suppressing vascular smooth cell activation and macrophage foam cell formation
Authors: Xia Weiwei , Li Yuanyuan , Wu Mengying , Yin Jie , Zhang Yue , Chen Hongbing , Huang Songming , Jia Zhanjun , Zhang Aihua 
Published: May 2019

AbstractBackgroundAtherosclerosis involves disorders in lipoprotein metabolism and inflammation. Mitochondrial dysfunction plays a critical role in promoting cell apoptosis, inflammation, and oxidative stress involved in the progress of atherosclerosis. Whereas the direct effect of mitochondrial activity modulation on atherogenesis remains unclear. This study evaluated the effect of a mitochondrial complex inhibitor on atherosclerosis in ApoE‐deficient mice as well as the potential mechanisms.Methods and ResultsWe treated ApoE‐deficient mice with mitochondrial complex I inhibitor rotenone in the western diet and found that rotenone attenuated early and advanced atherosclerosis with no effect on serum lipoprotein levels. Mechanistic investigation showed that rotenone suppressed primary macrophage foam cell formation possibly by suppressing CD36. In addition, we also found that the inhibitory effect of rotenone on VSMC proliferation and migration possibly by targeting the PI3K/AKT signaling. Consistently, mitochondrial complex III inhibitor azoxystrobin also exhibited similar actions as rotenone in VSMCs but not in macrophages.ConclusionsInhibition of mitochondrial activity could significantly attenuate atherosclerosis possibly by modulating CD36‐mediated macrophage foam cell formation and PI3K/AKT signaling pathway‐associated VSMC activation. Targeting mitochondrial activity might be the potential therapeutic strategy for atherosclerosis.
MicroRNA‐181b‐5p modulates tumor necrosis factor‐α‐induced inflammatory responses by targeting interleukin‐6 in cementoblasts
Authors: Wang Xiaoxuan , Sun Hualing , Liu Huan , Ma Li , Jiang Chenxi , Liao Haiqing , Xu Shihan , Xiang Junbo , Cao Zhengguo 
Published: May 2019

AbstractTooth cementum is a bone‐like mineralized tissue and serves as a microbial barrier against invasion and destruction. Cementum is also responsible for tooth stability and defending pulp from outside stimuli, which is formed by cementoblasts. Although it is crucial for periodontal and periapical diseases, the mechanisms underlying the pathophysiological changes of cementoblasts and their inflammatory responses remain unclear. MiR‐181b is found to modulate vascular inflammation and endotoxin tolerance. In this study, miR‐181b‐5p was downregulated in tumor necrosis factor‐α (TNF‐α)‐stimulated cementoblasts, whereas proinflammatory molecules increased. The mouse periapical lesions have similar results, which imitate an inflammatory environment for cementoblasts in vivo. The bioinformatics analysis and dual luciferase reporter assay suggested that miR‐181b‐5p targeted interleukin‐6 (IL‐6). Overexpressing miR‐181b‐5p negatively regulated IL‐6 and proinflammatory chemokine. Western blot analysis and luciferase activity reporter assay verified that miR‐181b‐5p weakened the NF‐κB activity. Hence, miR‐181b‐5p moderated proinflammatory chemokine production by targeting IL‐6 in cementoblasts and NF‐κB signaling pathway was involved. Furthermore, miR‐181b‐5p promoted cementoblast apoptosis, which may enhance the resolution of inflammation. Overall, our data revealed that miR‐181b‐5p was a negative regulator of TNF‐α‐induced inflammatory responses in cementoblasts.
Circular RNA circMAN2B2 facilitates glioma progression by regulating the miR‐1205/S100A8 axis
Authors: Xiong Jinsheng , Wang Tianyao , Tang Haitao , Lv Zhonghua , Liang Peng 
Published: May 2019

AbstractThe aim of this study was to research the mechanism of circMAN2B2 in the development of glioma. In our study, we found that circMAN2B2 has a higher expression in glioma tissues and cells, which was negatively related to the overall survival of glioma patients. The cell counting kit‐8 assay, 5‐ethynyl‐2′‐deoxyuridine labeling assay, transwell assay, and the nude mice assay indicated that knockdown of circMAN2B2 inhibited the cell proliferation, invasion, migration and decreased tumor size. In terms of mechanism, knockdown of circMAN2B2 increased the expression of miR‐1205. Moreover, circMAN2B2 regulated S100A8 expression by inhibiting miR‐1205. We also showed that knockdown of S100A8 inhibited cell proliferation, invasion, and migration. Increasing S100A8 expression rescued the effect of si‐circMAN2B2. In conclusion, circMAN2B2 could improve cell proliferation, invasion, and migration of the glioma by inhibiting miR‐1205 and promoting the expression of S100A8.
Dysregulated microRNAs in colorectal carcinogenesis: New insight to cell survival and apoptosis regulation
Authors: Anvarnia Alireza , Mohaddes‐Gharamaleki Farzad , Asadi Milad , Akbari Morteza , Yousefi Bahman , Shanehbandi Dariush 
Published: May 2019

AbstractColorectal cancer (CRC) is the third most common cancer and a leading cause of cancer‐related death worldwide. About 1.8 million new cases were reported in 2018. Accordingly, there is an urgent need for development of effective strategies to lower the mortality and morbidity originated from this malignancy. MicroRNAs (miRNAs) play key roles in several cellular events. They have attracted a great deal of attention in the etiology of a variety of disorders including cancer and might be considered as disease biomarkers or treatment targets. Initiation and development of CRC is a multistep process that is caused by loss of function (LOF) or gain of function (GOF) in genes of different signaling pathways. Particular miRNAs conduct oncogenic impacts by suppressing their target transcripts and simulate the effects of LOF or GOF, whereas there may be no sign of common genetic or epigenetic modifications. On the contrary, some miRNAs serve as tumor suppressor and help to maintain normal functioning and cellular homeostasis. Hence, highlighting the function of each individual miRNA in CRC tumorigenesis could assign it as a therapeutic target or molecular marker for further investigations. In the present review, we have tried to compile the latest information regarding the dysregulated miRNAs in CRC and their function in different disease stages and signaling pathways involved.
Long noncoding RNA LINC‐PINT regulates laryngeal carcinoma cell stemness and chemoresistance through miR‐425‐5p/PTCH1/SHH axis
Authors: Yuan Zhennan , Xiu Cheng , Liu Daming , Zhou Guangkai , Yang Hao , Pei Rong , Ding Chao , Cui Xiaowei , Sun Ji , Song Kaibin 
Published: May 2019

AbstractFunctional, noncoding RNA of about 200 nucleotides in length are known as long noncoding RNA (lncRNA). Advances in ‐omics have revolutionized the information with respect to the coding and noncoding regions of the genome. Several studies have illustrated the role of lncRNA in cell growth and cancer. Profiling and bioinformatic studies of laryngeal cancer has identified LINC‐PINT as one of the lncRNA. However, the functional aspects of the deregulation have not been studied in laryngeal tumors. In this study, LINC‐PINT expression in normal and tumor tissues were studied. Using a bioinformatic approach, microRNA (miRNA) targets of LINC‐PINT and gene targets of the miRNA were determined. The impact of LINC‐PINT on cell proliferation and chemoresistance was determined. Further through a set of silencing and re‐expression studies phenotype rescue was studied. LINC‐PINT expression was downregulated in laryngeal tumors. LINC‐PINT targeted miR‐425‐5p by three sites. miR‐425‐5p also targeted PTCH1 a protein of the Hedgehog pathway. Downregulation of LINC‐PINT was associated with increased cancer stemness and chemoresistance to cisplatin. Our results indicate a probable role of LINC‐PINT in the pathology of laryngeal tumors. LINC‐PINT re‐expression in laryngeal tumors may be explored for reversion of cancer cell stemness and also for rescue of drug resistance phenotype.
Effects of Sitagliptin on Lipid Profile in Patients With Type 2 Diabetes Mellitus After 7 Years of Therapy
Authors: Derosa Giuseppe , Tritto Isabella , Romano Davide , D'Angelo Angela , Catena Gabriele , Maffioli Pamela 
Published: May 2019

AbstractThe aim of this study was to investigate whether the effects of sitagliptin on lipid profile were maintained even after 7 years of treatment. We treated 591 patients who had not been well controlled by current therapy with the addition of sitagliptin 100 mg/d. Data were compared with those of 612 patients treated with sulfonylureas plus metformin, pioglitazone plus metformin, and pioglitazone plus sulfonylureas. We observed that, compared with patients treated with sulfonylureas plus metformin, patients receiving sitagliptin in addition to metformin experienced a greater decrease of total cholesterol and low‐density lipoprotein cholesterol (LDL‐C) compared with baseline and with sulfonylureas plus metformin. Compared with patients treated with metformin plus pioglitazone, sitagliptin resulted in a greater reduction of total cholesterol and LDL‐C relative to baseline and to metformin plus pioglitazone. We also observed a higher increase of high‐density lipoprotein cholesterol (HDL‐C) after sitagliptin had been added to pioglitazone, both compared with baseline and to the competitor. Compared with patients treated with pioglitazone plus sulfonylureas, the combination of sitagliptin and sulfonylureas was more effective in reducing LDL‐C and in increasing HDL‐C. High‐sensitivity C‐reactive protein was decreased by all pharmacological combinations. We can conclude that the addition of sitagliptin led to a better and more durable improvement of lipid profile compared with sulfonylureas or thiazolidinediones.
Food wastes and sewage sludge as feedstock for an urban biorefinery producing biofuels and added‐value bioproducts
Authors: Battista Federico , Frison Nicola , Pavan Paolo , Cavinato Cristina , Gottardo Marco , Fatone Francesco , Eusebi Anna L , Majone Mauro , Zeppilli Marco , Valentino Francesco , Fino Debora , Tommasi Tonia , Bolzonella David 
Published: May 2019

AbstractThe updated Bioeconomy Strategy document “A sustainable bioeconomy for Europe: strengthening the connection between economy, society and the environment”, which was issued by the European Commission in October 2018, encourages the exploitation of organic wastes according to a pyramidal hierarchy in which the extraction of valuable biomolecules, which will be used as they are or as precursors of high‐added‐value compounds, is a priority in biofuel production. This review considers a biorefinery platform in which food waste and sewage sludge are adopted to produce volatile fatty acids (VFAs) through a dark fermentation process. VFA fermentation is optimized by slightly acid pH (6–7), short hydraulic retention time (1–7 days) and high organic load rate (more than 10 gTS L−1 d−1). Attention has been focused on VFA exploitation for polyhydroxyalkanoate (PHA) production via a ‘feast and famine’ strategy performed in sequencing batch reactors. The obtained PHA yields are around 0.4–0.5 gPHA gCOD−1. Moreover, VFAs allow for the production of biofuels, such as hydrogen and methane, through single‐ or double‐staged anaerobic digestion. Innovative bioelectrochemical upgrade strategies for biogas helps producers to obtain biomethane for the automotive sector. Moreover, biogas has recently been tested for the production of polyhydroxybutyrate, a biodegradable and biocompatible thermoplastic made by microorganisms from C1 carbon sources (CO2 and CH4). Digestates from anaerobic bioreactors are still rich in nitrogen and phosphorus compounds. These latter compounds have been identified as critical raw materials due to their low availability in the European Union and to increasing demand from the growing global population. Thus, nutrient recovery from digestate allows users to close the loop of the ‘circular economy’ approach. © 2019 Society of Chemical Industry