bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of porcine granulocyte-macrophage-colony-stimulating factor on porcine cumulus-oocyte complex maturation in vitro
Authors: Dang Long Thanh , Le Thu-Thao Thi , Huynh Cam Manh , Hoang Son Nghia 
Published: January 2019

Introduction: The in vitro maturation (IVM) of oocytes is an essential step in the in vitro embryo production. This study aimed to improve the efficacy of IVM by supplementing with granulocytemacrophage colony stimulating factor (GM-CSF) in the culture medium.
Methods: The combinatorial effects of GM-CSF and porcine follicular fluid (pFF) were also investigated in porcine cumulusoocyte complex (COC) models. COCs were obtained by aspirating follicles with an 18 G needles. Small and medium follicles were used in this study. COCs from two kinds of follicles were divided into 13 groups to investigate the maturation of COCs at different concentrations of pFF (0, 10%) and GM-CSF (0, 2, 10, 20 ng/mL). The maturation of COCs was evaluated by different variables including cumulus expansion, the appearance of the first polar body, lipid droplet localization, mitochondrial distribution, and cortical granule distribution.
Results: The results showed that GM-CSF improves the cumulus expansion, and stimulate the mitochondrial and cortical granule movement. However, GM-CSF did not increase nuclear maturation or lipid droplet localization rates in the porcine oocyte.
Conclusion: In conclusion, porcine GM-CSF had some positive effects on the porcine oocyte maturation.
Diagnosis and Treatment Monitoring of a Patient with Gastrointestinal Stromal Tumor by Next-Generation Sequencing and Droplet Digital Polymerase Chain Reaction Assay of a PDGFRA Mutation in Plasma-Derived Cell-Free Tumor DNA
Authors: Boonstra Pieter A. , ter Elst Arja , Tibbesma Marco , Gietema Jourik A. , Schuuring Ed , Reyners Anna K.L. 
Published: January 2019

Abstract
In patients with a suspected malignancy, standard-of care management currently includes histopathologic examination and analysis of tumor-specific molecular abnormalities. Herein, we present a 77-year-old patient with an abdominal mass suspected to be a gastrointestinal stromal tumor (GIST) but without the possibility to collect a tumor biopsy. Cell-free DNA extracted from a blood sample was analyzed for the presence of mutations in GIST-specific genes using next generation sequencing. Furthermore, liquid biopsies were used to monitor the levels of mutant DNA copies during treatment with a tumor-specific mutation droplet digital PCR assay that correlated with the clinical and radiological response. Blood-based testing is a good alternative for biopsy-based testing. However, it should only be applied when biopsies are not available or possible to obtain because overall, in only 50%–85% of the cell-free plasma samples is the known tumor mutation detected.
Rio+20 : Quelle ambition pour l'environnement? 3eme réunion mondiale de juristes et des associations de droit de l'environnement, Limoges, les 29, 30 septembre et 1er octobre 2011 - Recommandations issues de la réunion
Authors: Droit Comparé de l'Environnement Centre International de 
Published: January 2019

RIO+20 : QUELLE AMBITION POUR L'ENVIRONNEMENT ?3eme réunion mondiale de juristes et des associations de droit de l'environnement, Limoges les 29, 30 septembre et 1er octobre 2011 - Recommandations issues de la réunion RIO+20: WHAT AMBITIONS FOR THE ENVIRONMENT?3rd World Meeting of Environmental Lawyers and Environmental Law Associations, Limoges, 29 September - 1st October 2011 - Recommendations adopted in the Meeting RIO+20: ¿QUÉ AMBICIÓN PARA EL MEDIO AMBIENTE?3ª reunión mundial de juristas y de asociaciones de Derecho ambiental, Limoges, 29 de septiembre a 1 de octubre de 2011 -Recomendaciones adoptadas en la reunión
Conflictividad y desplazamiento ambiental: elementos jurídico-políticos de justicia ambiental y reparación a sociedades tradicionales
Authors: Mesa Cuadros Gregorio 
Published: January 2019

El proceso de desarrollo agenciado por el modelo hegemónico de apropiación de la naturaleza recurre a proyectos y megaproyectos de extracción que, en lo sustantivo, generan desplazamiento ambiental de pueblos, sociedades y comunidades tradicionales rurales y agrarias que habitan ancestralmente bosques y territorios colectivos. En este escrito presentamos algunos de los momentos más significativos del desplazamiento ambiental resultado de las actividades humanas que generan proyectos, obras o actividades de empresas alrededor del mundo y que, en las últimas décadas, usan sin límite o control los bosques naturales, el aire, los suelos, el subsuelo y demás elementos del ambiente. Paradójicamente, las sociedades tradicionales, que han conservado los bosques, el agua y los suelos por muchos años, son violentadas en sus derechos y tienen que huir o desplazarse de sus territorios tradicionales por un modelo de desarrollo inadecuado basado en huellas ambientales y energéticas insostenibles. Este modelo ha establecido que todo puede ser apropiado en la naturaleza y que los límites ambientales podrían ir en contra del desarrollo y los derechos de las personas, desconociendo la cruda realidad del deterioro ambiental que afecta a los más vulnerables, que terminan desplazados forzadamente de sus tierras y se convierten en "migrantes climáticos". Nuestra reflexión se desarrolla en dos momentos: el primero establece las formas de apropiación de la naturaleza que llevan a la degradación y contaminación de elementos ambientales clave para la vida presente y futura y sus consecuencias ambientales (ecosistémicas y culturales) sobre las sociedades tradicionales que habitan bosques naturales; el segundo recupera el papel de estas sociedades tradicionales en la conservación ambiental y propone una visión alternativa desde el Derecho ambiental para el reconocimiento y la compensación como sujetos colectivos vitales en la resolución de los conflictos y problemas globales actuales y en la concreción de una idea de dignidad y justicia ambiental.
Perspectives de la litigació pel canvi climàtic arran del cas Urgenda
Authors: Ruda-Gonzalez Albert 
Published: January 2019

La sentència del tribunal de l'Haia de juny de 2016 en el cas Urgenda, que va condemnar l’Estat holandès per no fer prou per combatre el canvi climàtic,va suposar un autèntic terratrèmol per al Dret de la responsabilitat ambiental i va donar lloc a una progènie de reclamacions similars. La seva confirmació pel tribunal d’apel·lació corresponent el passat 9 d’octubre de 2018 sembla donar-hi encara més força. No obstant això, el fet és que aquests pronunciaments judicials susciten qüestions molt profundes no només sobre la responsabilitat (civil o patrimonial de l'Administració, segons el sistema) sinó també d'ordre constitucional. En judicialitzar un tema eminentment polític, permeten de plantejar dubtes sobre si pronunciaments com aquests respecten o no la deguda separació entre els poders de l'Estat. Aquest article analitza críticament unes sentències que, si bé han donat una gran visibilitat a una qüestió de gran urgència i encara d’actualitat, sembla pressuposar que un tribunal és el millor lloc per a jutjar la responsabilitat pel canvi climàtic. Aquest treball s'acaba preguntant si una reclamació “a la Urgenda”seria possible en el nostre sistema i quin seria previsiblement el seu resultat.
Law of the Sea and Environmental Law acting together: Experience of laying submarine cable in the Arctic
Authors: Shvets Daria 
Published: January 2019

All states have the right to lay submarine cables on the seabed according to the United Nations Law of the Sea Convention 1982 (LOSC). Unlike submarine pipelines, cables are generally consideredbeing environmentally friendly, having minimum impact on the marine environment. Currently, there are several projects on submarine cables planned to be laidin the Arctic. In particular, the Quintillion Subsea Cable System is an ongoinglarge-scale project, which majorpart will go through the Arctic waters. Despite mostly local and moderate disturbance to the environment in other regions, it causes certainconcerns regarding the influence on the Arctic environment due to its unique and sensible ecosystem. The LOSC does notseparate the Arctic from other regions, andcables are laid there following the same conditions as well as in other parts of the World Ocean. This article aims to examine the international legal regime of submarine cables in the context of the ongoing cable projects in the Arctic as well as to consider the applicability of the precautionary principle and the provisions of the Polar Code for ensuring the protection of the Arctic environment.
High-dose chemotherapy with autologous hematopoietic stem cell transplantation in high-risk neuroblastoma patients: Raisa Gorbacheva Memorial Research Institute of Children Oncology, Hematology and Transplantation, First Pavlov State Medical University of St. Petersburg experience
Authors: Kazantsev I. V. , Gevorgyan A. G. , Yukhta T. V. , Tolkunova P. S. , Kozlov A. V. , Andreeva T. V. , Babenko E. V. , Estrina M. A. , Kuga P. S. , Litvinov A. P. , Lapekin S. V. , Safonova S. A. , Punanov Yu. A. , Zubarovskaya L. S. , Afanasyev B. V. 
Published: January 2019

Introduction.Neuroblastoma (NB) is the most common extracranial pediatric solid tumor. The high-risk group patients are characterized by adverse prognosis and require intensive complex therapy including high-dose chemotherapy (HDCT) with hematopoietic stem cell transplantation (auto-HSCT). The current study presents a single center experience of HSCT with auto-HSCT for high-risk NB performed in Raisa Gorbacheva Memorial Research Institute of Children Oncology, Hematology and Transplantation, First Pavlov State Medical University of St. Petersburg, Ministry of Health of Russia.Patients and methods.A cohort of 72 consecutive high-risk NB patients was included in the study. Among them 69 patients received Bu-Mel conditioning regimen (busulfan 16 mg/kg, melphalan 140 mg/m2 ), in 3 patients the 5D/5D regimen was used (carboplatin 1000 mg/m2 , irinotecan 150 mg/m2 , temozolomide 750 mg/m2 , etoposide 400 mg/m2 , cyclophosphamide 140 mg/kg). In most cases the autologous hematopoietic stem cells source was bone marrow (BM) (n = 59; 82 %), peripheral blood stem cells (PBSC) (n = 11; 15 %), or BM and PBSC (n = 2; 3 %). In 52/66 (79 %) patients with initial bone marrow involvement the potential transplant contamination was assessed by flow cytometry.Results.The 2-year and 5-year overall (OS) and event-free (EFS) survival was 61 % and 48 %, 41 % and 35 % accordingly. The main adverse factors for OS and EFS were age of more than 18 months at diagnosis, combined bone marrow and bones involvement, MYCN amplification, initial neuron-specific enolase level of more than 100 ng/ml, primary resistance or relapse, and metaiodobenzylguanidinepositive lesions persistence prior to or after HSCT with auto-HSCT.Conclusions.The results achieved are comparable to those described for similar cohorts. Some patient subgroups are unlikely to achieve response after HSCT with auto-HSCT. Therefore, additional stratification methods and treatment modalities are needed.Conflict of interest. The authors declare no conflict of interest.Funding. The study was performed without external funding.
Atypical teratoid/rhabdoid tumors of the central nervous system in children: the state of the problem today. Literature review
Authors: Dinikina Yu. V. , Belogurova M. B. 
Published: January 2019

Atypical teratoid/rhabdoid tumors (AT/RT) are a group of rare highly aggressive malignant tumors in young patients. Among all the malignant tumors of the central nervous system (CNS) in children, they are 1–2 %, which, due to the small number of groups, makes it difficult to develop uniform recommendations for antitumor therapy. The molecular genetic profile of AT/RT, which largely determines the characteristics of the disease, has been studied sufficiently. Despite the large number of ongoing clinical studies, the results of treatment of AT/RT CNS in the world today remain unsatisfactory. The early age of patients limits the use of radiation therapy, which leads to the need to intensify chemotherapy regimens and to choose the optimal strategy in the toxicity – benefit ratio. The article describes modern approaches to the treatment of central nervous system disorders in children, presents the results of studies with the largest number of included patients, using the multimodal treatment strategy, identifies current trends in targeted therapy.Conflict of interest. The authors declare no conflict of interest.Funding. The study was performed without external funding.
Successful targeted therapy in a patient with transformation of a BRAF-mutated ameloblastoma into an undifferentiated round-cell sarcoma
Authors: Sagoyan G. B. , Mareeva Yu. M. , Roshchin V. Yu. , Grachev N. S. , Konovalov D. M. , Druy A. E. , Bondarenko V. P. , Alekseev M. A. , Ovsyannikova G. S. , Litvinov D. V. , Zhukov N. V. 
Published: January 2019

The article describes a clinical case of a patient aged 15 years with a sequential transformation of the ameloblastoma of the upper jaw into ameloblastic fibrosarcoma and undifferentiated round-cell sarcoma as a result of frequent relapses against the background of standard anticancer treatment. This clinical example is of interest not only because of the preservation of the mutation in the BRAF gene at all stages of transformation, which made it possible to conduct successful therapy with BRAF and MEK inhibitors after the exhaustion of all standard therapeutic possibilities.Conflict of interest. The authors declare no conflict of interest.Funding. The study was performed without external funding
The first experience of using immunotherapy in a patient with a recurrence of neuroblastoma
Authors: Zvyagintseva D. A. , Kuleva S. A. , Ivanova S. V. 
Published: January 2019

Neuroblastoma (NB) is one of the most common childhood solid tumors. Current problem of children’s oncology is treatment of patients from high-risk NB. Now the standard of treatment of these patients is application of the stage-by-stage therapy including induction, consolidation, and post-consolidation. During induction the maximum reduction of the primary tumor and the metastasis for a possibility of optimum local control is reached. Consolidation consists in high-dose chemotherapy which purpose is to consolidate the reached remission. After the completions of treatment patients receive the maintenance therapy, the standard is use of the differentiating therapy in combination with monoclonal anti-GD2-antibody (dinutuximab). Certainly, this tactics allowed achieving considerable progress in treatment of primary patients with unfavorable prognosis. Options of treatment for high-risk NB depend on some factors. Certainly, the tumor dissemination is characterized by extremely adverse forecast for life and recovery of the patient. However a local recurrence can be successfully cured. The purpose of this study is to demonstrate a rare clinical case of therapy from isolated central nervous system NB recurrence.Conflict of interest. The authors declare no conflict of interest.Funding. The study was performed without external funding.
A Formação Inicial de Professores no EaD e a Iniciação Científica: a Experiência do Projeto de Pesquisa Professor seu Lugar é Aqui EAD
Authors: Battini Okçana , Salvi Vinicius , Reis Sandra Regina 
Published: January 2019

No momento atual, o crescimento da Educação a Distância (EaD) atende outros direcionamentos. A oferta, que tinha forte vinculação somente com o ensino, passa a se estender à pesquisa e à extensão. As universidades públicas e privadas começam a investir em pesquisa com aluno de EaD e inserir programas de iniciação científica nesta modalidade. Nos cursos de formação de professores na EaD essa tendência tende a ser mais presente e apontam para a necessidade de fortalecimento. As questões aqui apontadas decorrem de um recorte do projeto de Pesquisa: “Professor seu lugar é aqui EaD: estudos mediados pelas tecnologias sobre a formação, trabalho e práticas pedagógicas no espaço escolar”. O recorte apresentado recai sobre a descrição de como foram realizadas as atividades com os alunos de Iniciação Científica matriculados nos cursos de Licenciatura EaD da Unopar. É importante salientar que o atendimento ao estudante de iniciação cientifica EaD foi realizado por meio das tecnologias da informação e da comunicação, via AVA, sendo acompanhado por professores doutores e alunos do Mestrado em Ensino da Universidade. As discussões apresentadas demonstram o ineditismo de ações de Iniciação Científica no Ensino a Distância e apontam para caminhos possíveis para se expandir e consolidar este processo na EaD.Palavras chaves: Iniciação Científica. Modalidade Ensino a Distância. Formação Inicial de Professores.AbstractNowadays, the growth of distance education (EAD) serves other directions. The offer that had a strong connection only with teaching began to be extended to research and extension. Public and private universities begin to invest in research with EaD students and to introduce scientific initiation programs in this modality. In teacher training courses in EaD this trend tends to be more present and point to the need for strengthening. The questions mentioned in the study herein derive from a cut-off of the research project “Professor seu lugar é Aqui Ead”: studies mediated by technologies on training, work and pedagogical practices in the school space”. The cut-off exhibited , falls on the description of how the activities were carried out with the students of Scientific Initiation enrolled in the courses of Bachelor Degree EADS of Unopar. It is important to point out that the service to the scientific initiation student EaD was carried out through the information and communication technologies, via AVA, being followed up by professors doctors and students of the Master’s degree Teaching of the University. The discussions presented demonstrate the novelty of Scientific Initiation actions in Distance Learning and point to possible ways to expand and consolidate this process in EaD.Keywords: Scientific Initiation. Distance Learning Modality. Initial Teacher’s Training.
Türkiye’de Sığır Eti Üretimi ve Dış Ticaretinde Son 25 Yıllık Tarımsal Politikaların ve Uygulamaların Değerlendirilmesi
Authors: NİYAZ Özge Can 
Published: January 2019

Gelişmiş
toplumların beslenme biçimleri incelendiğinde hayvansal protein tüketiminin
gelişmemiş toplumlara göre oldukça fazla olduğu görülmektedir. Yeterli miktarda
protein tüketebilmek beslenme anlamında bir gelişmişlik göstergesidir.
Ulusların yeterli miktarda protein alımı içeren beslenme düzenlerine sahip
olması ise bu alandaki tarımsal politikalar ve bu politikaların uygulamaları
ile doğrudan ilişkilidir. Türkiye’de kişi başına hayvansal protein tüketimi
gelişmiş ülkelerin oldukça gerisinde kalmaktadır. Bunun temel sebebi ise
kırmızı et üretim ve dış ticaret politikalarının doğru belirlenememesi veya
uygulanamamasıdır. Kırmızı et üreticisi ve tüketicisi bu aksaklıklardan
doğrudan etkilenmektedir. Bu nedenle, bu çalışmanın amacı Türkiye’nin son 25
yıllık döneminde sığır eti üretim ve dış ticaretinde belirlenen ve uygulanan
politikaların irdelenmesidir. Çalışmada resmi raporlar ve akademik
çalışmalardan alınan veriler derlenmiştir. Sığır eti üretimi yıllar itibari ile
incelendiğinde mevcut tüketim düzeyinde yeterli görülmektedir. Fakat Türkiye’de
kırmızı et tüketimi gelişmiş ülkelerin gerisindedir. Buna göre daha yüksek bir
tüketim düzeyi için mevcut sığır üretiminin de yetersiz kaldığı görülmektedir. Dış ticaret verileri incelendiğinde, 2010
yılında başlayan sığır eti ithalatın uzun vadede tüketici fiyatlarında düşüşün
aksine fiyat düzeyinde artışa sebep olduğu görülmüştür. Artan girdi maliyetleri
ve ithalat yerli sığır yetiştiricilerinin önünde duran en büyük engellerdir.
Buna göre; Türkiye’nin sığır eti ihtiyacının karşılanması yerli üreticiyi
destekleyecek politikaların güçlendirilmesi yolu ile yapılmalıdır. Et Balık
Kurumu et işleme kapasitesini nüfusun ihtiyacını karşılayacak şekilde
genişletmeli ve tekrar piyasa üzerinde aktif rol oynar hale getirilmelidir.
Cultural investment of Javanese Krama language in Islamic elementary schools
Authors: Khuzaefi Khulukul 
Published: January 2019

Language is the most essential communication tool for human beings. Languages possessed by humans are so dynamic that it continues to evolve over time. The language instruction was termed Linguistic Etiquette. In Linguistic Etiquette there is a term of speech levels. The use of language in Islamic Elementary School (Madrasa Ibtidaiyah/MI) was implemented in an integrated manner with the relationshipbetween fields of study. Javanese is the mother tongue of the Javanese. The society levels of speech in Javanese language are Ngoko, Krama Madya, and Krama Inggil. The Javanese practices Krama level language to communicate between students with teachers, among teachers and teachers with headmaster of MI at school. This research was conducted qualitatively to informants from MI Tegalwaton and MI Al Islam Bonomerto. The researcher used qualitative descriptive approach to describe the state or phenomenon status of the data obtained from the research object, which then analyzed. This study are investigating MI programs in instilling Javanese `Krama` language, the results of Javanese `Krama` language cultural cultivation in MI Tegalwaton and MI Al Islam Bonomerto as well as obstacles experienced by the students in using Javanese `Krama` language.
Building a Community of Connected ELT Professionals on Twitter
Authors: Nicholas Bonnie , Avram Augusta , Chow Jennifer , Lupasco Svetlana 
Published: January 2019

Although English language teaching professionals are committed to ongoing continuous professional development, economic and workload factors may impede their ability to attend a traditional 2-day annual conference. Teachers have turned to social media as an alternative approach to self-directed professional development. In particular, Twitter has become the choice of many educators with its potential for networking and collaboration. There are scheduled Twitter chats held regularly on a variety of topics of interest to language teaching professionals, including #CdnELTchat. In addition, connected educators have built a community of practice on the Twitter platform and provide inspiration and support to their colleagues online. In this article, four connected educators who have found their community on Twitter share their stories of how they got started on Twitter, how they connected with each other, and what they value about the open Twitter platform.
Bien que les professionnels de l’enseignement de l’anglais s’engagent à poursuivre un perfectionnement professionnel continu, des facteurs économiques ou des charges de travail peuvent les empêcher d’assister à une conférence annuelle traditionnelle de 2 jours. Les enseignantes et enseignants trouvent dans les réseaux sociaux une solution de rechange qui leur permet de veiller à leur propre perfectionnement professionnel. Twitter, notamment, est le nouveau choix de nombreux éducateurs et éducatrices en raison des possibilités de réseautage et de collaboration qu’il présente. Des sessions de clavardage ont régulièrement lieu sur une variété de sujets présentant un intérêt pour les professionnels de l’enseignement des langues, et ce, notamment sur #CdnELTchat. Qui plus est, les éducatrices et éducateurs connectés ont construit une communauté de praticiens sur la plateforme Twitter et fournissent une inspiration et un soutien à leurs collègues en ligne. Dans cet article, quatre éducatrices connectées qui ont découvert leur communauté sur Twitter décrivent comment elles ont commencé à se servir de Twitter, comment elles se sont connectées les unes aux autres, et ce qu’elles apprécient le plus sur la plateforme ouverte de Twitter.