bluuebunny/crossref_metadata_2025_split huggingface dataset:
Duodenal Bulb Histology in Paediatric Celiac Disease: A Case–Control Study
Authors: Boschee Erin , Lacson Atilano , Turner Justine , Yap Jason 
Published: April 2019

Abstract

Background
Controversy exists about optimal methods for duodenal biopsy in diagnosis of celiac disease (CD), in terms of both number of samples and anatomic location. The reliability of duodenal bulb biopsy has been questioned given that normal bulb architecture may mimic disease. However, multiple studies have reported patients with CD have histopathological lesions limited to proximal changes in the duodenal bulb alone.

Methods
We retrospectively compared duodenal and duodenal bulb histology in a population of paediatric patients with CD and compared with a population of nonceliac controls at Stollery Children’s Hospital, 2010 to 2012.

Results
Fifty-seven paediatric patients diagnosed with CD and 16 nonceliac controls were included in the study. Fifty-three celiac patients (93.0%) had histopathology consistent with CD (modified Marsh score of 3A, 3B or 3C) in the duodenal bulb. The modified Marsh classification differed significantly between duodenum and duodenal bulb in nine celiac patients (15.8%). Of these, five (8.8%) had Marsh 3 in the bulb and Marsh 0 in the distal duodenum. Among controls, no patients had villous atrophy in either the distal duodenum or duodenal bulb, and all patients had a modified Marsh score of 0 at both sites.

Conclusions
The results of this study reinforce that duodenal bulb samples are critically important for diagnosing CD in paediatric patients. We suggest that duodenal bulb samples be submitted in separate containers from distal duodenal samples to facilitate accurate interpretation. In contrast to prior reports, we found villous blunting and intraepithelial lymphocytosis are actually uncommon findings in paediatric patients with nonceliac gastrointestinal disorders.
Sexual maturation in male long-finned pilot whales (Globicephala melas edwardii): defining indicators of sexual maturity
Authors: Betty Emma L , Stockin Karen A , Smith Adam N H , Bollard Barbara , Orams Mark B , Murphy Sinéad 
Published: April 2019

AbstractMale reproductive biology is described for the Southern Hemisphere long-finned pilot whale (Globicephala melas edwardii), a subspecies that regularly mass strands along the New Zealand coastline. Ten mass stranding events sampled over a 7-year period enabled assessments of key life history parameters. Sexual maturation in immature, maturing, and mature males was assessed using morphological data and histological examination of testicular tissue. Variation was observed in the age (11–15 years) and length (450–490 cm) at which individuals attained sexual maturity. Using Bayesian cumulative logit regression models, we estimated the average age and length at the attainment of sexual maturity to be 13.5 years and 472 cm, respectively. Combined testes weight, combined testes length, an index of testicular development (combined testes weight/combined testes length), and mean seminiferous tubule diameter were all good indicators of sexual maturity status. Combined testes length was the best nonhistological indicator, and all testicular measures were found to be better indicators of sexual maturation for G. m. edwardii than age or total body length. Sexual maturity was attained before physical maturity (> 40 years and 570 cm), and at a younger age and smaller body length than previously reported for Globicephala melas melas in the North Atlantic. Given the ease of collection, minimal processing, and applicability to suboptimal material collected from stranding events, future studies should assess the value of testicular size as an indicator of sexual maturity in pilot whales and other cetacean species. Estimates of the average age and length at sexual maturity for G. m. edwardii provided in this study may be used to inform population models required for conservation management of the subspecies, which is subject to high levels of stranding-related mortality.
Development of Chrysomya rufifacies (Diptera: Calliphoridae) at Constant Temperatures Within its Colony Range in Yangtze River Delta Region of China
Authors: Hu Guoliang , Wang Yu , Sun Yong , Zhang Yingna , Wang Man , Wang Jiangfeng 
Published: April 2019

Abstract
The age of insects found on corpses is often used to estimate the minimum postmortem interval by forensic entomologists. Insect development is affected by biotic and abiotic factors, and temperature is the most important environmental factor that determines the length of insect development. Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) is a globally distributed fly that is commonly found on corpses, and this study investigated the development of C. rufifacies from China at various constant temperatures. At 16, 19, 22, 25, 28, 31, and 34°C, the developmental time from egg to adult was 870.17 ± 11.50, 566.20 ± 8.79, 406.38 ± 10.98, 291.14 ± 4.71, 232.59 ± 5.96, 192.47 ± 3.45, and 160.48 ± 7.15 h, respectively. We established three developmental models for C. rufifacies to estimate the age of the developing insect: the isomorphen diagram model, the isomegalen diagram model and the thermal summation model. Regression analysis was conducted to obtain the relationship between body length and development time of the larvae from hatching to wandering. The developmental threshold temperatures of the egg, 1st instar, 2nd instar, 3rd instar, and wandering larvae, and intra-puparial stages were 12.28 ± 0.30, 11.74 ± 0.95, 12.70 ± 0.55, 11.68 ± 0.96, 10.53 ± 1.53, and 12.51 ± 0.41°C, respectively. The developmental threshold temperature, and the thermal summation constant during the entire developmental period were 3759.95 ± 170.80 degree hours and 11.96 ± 0.38°C, respectively. This study provides an improved dataset to estimate the postmortem interval of corpses using C. rufifacies.
Resistance Status to Deltamethrin, Permethrin, and Temephos Along With Preliminary Resistance Mechanism in Aedes aegypti (Diptera: Culicidae) From Punjab, Pakistan
Authors: Khan Hafiz Azhar Ali , Akram Waseem 
Published: April 2019

Abstract
The use of insecticides such as deltamethrin, permethrin, and temephos has been a primary tool to manage Aedes aegypti (Linnaeus) in Punjab province, Pakistan; however, recent reports of control failure necessitate monitoring insecticide resistance. For this reason, we evaluated 12 field strains of Ae. aegypti from Punjab for resistance against deltamethrin, permethrin, and temephos along with underlying resistance mechanisms. For deltamethrin, high level of resistance was observed in Rawalpindi, Faisalabad, Sheikhupura, Lahore, Pattoki, and Kasur strains (RRLC50 > 10-fold); moderate level of resistance in Sargodha, Gujranwala, and Sialkot strains (RRLC50 = 5- to 10-fold), and low level of resistance in Okara, Multan, and Sahiwal strains (RRLC50 < 5-fold). In the case of permethrin, high level of resistance was found in all the field strains, except the Okara strain that exhibited moderate resistance. For temephos, five field strains, viz. Faisalabad, Rawalpindi, Kasur, Lahore, and Gujranwala, showed high level of resistance; five strains, viz. Sheikhupura, Sialkot, Pattoki, Sahiwal, and Okara, showed moderate resistance, and two strains from Multan and Sargodha showed low resistance to temephos. Synergism bioassays implementing piperonyl butoxide and S,S,S-tributylphosphorotrithioate exhibited a nonsignificant effect on synergizing toxicity of deltamethrin and permethrin in all field strains except the Lahore strain, suggesting the possible role of target-site resistance mechanism. However, both synergists had a significant effect on synergizing toxicity of temephos in all field strains, suggesting the possibility of metabolic-based mechanism of insecticide resistance. In conclusion, the study confirmed the presence of resistance to deltamethrin, permethrin, and temephos in the studied field strains of Ae. aegypti from Punjab, Pakistan.
A bilateral Hilbert-style investigation of 2-intuitionistic logic
Authors: Drobyshevich Sergey 
Published: April 2019

AbstractWe develop a bilateral Hilbert-style calculus for 2-intuitionistic logic of Heinrich Wansing. This calculus is defined over signed formulas of two types: formulas signed with plus correspond to assertions, while formulas signed with minus correspond to rejections. In this way, the provided system is a Hilbert-style calculus, which does take rejection seriously by considering it a primitive notion on par with assertion. We show that this presentation is not trivial and provide two equivalent axiomatizations obtained by extending intuitionistic and dual intuitionistic logics, respectively. Finally, we show that 2-intuitionistic logic is in some sense definitionally equivalent to a variant of Nelson’s logic with constructible falsity.
Addressing the circularity problem in the Ep−Eiso correlation of gamma-ray bursts
Authors: Amati Lorenzo , D’Agostino Rocco , Luongo Orlando , Muccino Marco , Tantalo Maria 
Published: April 2019

ABSTRACT
We here propose a new model-independent technique to overcome the circularity problem affecting the use of gamma-ray bursts (GRBs) as distance indicators through the use of Ep−Eiso correlation. We calibrate the Ep−Eiso correlation and find the GRB distance moduli that can be used to constrain dark energy models. We use observational Hubble data to approximate the cosmic evolution through Bézier parametric curve obtained through the linear combination of Bernstein basis polynomials. In doing so, we build up a new data set consisting of 193 GRB distance moduli. We combine this sample with the supernova JLA data set to test the standard ΛCDM model and its wCDM extension. We place observational constraints on the cosmological parameters through Markov Chain Monte Carlo numerical technique. Moreover, we compare the theoretical scenarios by performing the Akaike and Deviance Information statistical criteria.the 2σ level, while for the wCDM model we obtain $\Omega _m=0.34^{+0.13}_{-0.15}$ and $w=-0.86^{+0.36}_{-0.38}$ at the 2σ level. Our analysis suggests that ΛCDM model is statistically favoured over the wCDM scenario. No evidence for extension of the ΛCDM model is found.
The extruded non-template strand determines the architecture of R-loops
Authors: Carrasco-Salas Yeraldinne , Malapert Amélie , Sulthana Shaheen , Molcrette Bastien , Chazot-Franguiadakis Léa , Bernard Pascal , Chédin Frédéric , Faivre-Moskalenko Cendrine , Vanoosthuyse Vincent 
Published: April 2019

Abstract
Three-stranded R-loop structures have been associated with genomic instability phenotypes. What underlies their wide-ranging effects on genome stability remains poorly understood. Here we combined biochemical and atomic force microscopy approaches with single molecule R-loop footprinting to demonstrate that R-loops formed at the model Airn locus in vitro adopt a defined set of three-dimensional conformations characterized by distinct shapes and volumes, which we call R-loop objects. Interestingly, we show that these R-loop objects impose specific physical constraints on the DNA, as revealed by the presence of stereotypical angles in the surrounding DNA. Biochemical probing and mutagenesis experiments revealed that the formation of R-loop objects at Airn is dictated by the extruded non-template strand, suggesting that R-loops possess intrinsic sequence-driven properties. Consistent with this, we show that R-loops formed at the fission yeast gene sum3 do not form detectable R-loop objects. Our results reveal that R-loops differ by their architectures and that the organization of the non-template strand is a fundamental characteristic of R-loops, which could explain that only a subset of R-loops is associated with replication-dependent DNA breaks.
Fatty acid conjugation enhances potency of antisense oligonucleotides in muscle
Authors: Prakash Thazha P , Mullick Adam E , Lee Richard G , Yu Jinghua , Yeh Steve T , Low Audrey , Chappell Alfred E , Østergaard Michael E , Murray Sue , Gaus Hans J , Swayze Eric E , Seth Punit P 
Published: April 2019

Abstract
Enhancing the functional uptake of antisense oligonucleotide (ASO) in the muscle will be beneficial for developing ASO therapeutics targeting genes expressed in the muscle. We hypothesized that improving albumin binding will facilitate traversal of ASO from the blood compartment to the interstitium of the muscle tissues to enhance ASO functional uptake. We synthesized structurally diverse saturated and unsaturated fatty acid conjugated ASOs with a range of hydrophobicity. The binding affinity of ASO fatty acid conjugates to plasma proteins improved with fatty acid chain length and highest binding affinity was observed with ASO conjugates containing fatty acid chain length from 16 to 22 carbons. The degree of unsaturation or conformation of double bond appears to have no influence on protein binding or activity of ASO fatty acid conjugates. Activity of fatty acid ASO conjugates correlated with the affinity to albumin and the tightest albumin binder exhibited the highest activity improvement in muscle. Palmitic acid conjugation increases ASO plasma Cmax and improved delivery of ASO to interstitial space of mouse muscle. Conjugation of palmitic acid improved potency of DMPK, Cav3, CD36 and Malat-1 ASOs (3- to 7-fold) in mouse muscle. Our approach provides a foundation for developing more effective therapeutic ASOs for muscle disorders.
Brain Physiological Response and Adaptation During Spaceflight
Authors: Marshall-Goebel Karina , Damani Rahul , Bershad Eric M 
Published: April 2019

Abstract
More than half of astronauts returning from long-duration missions on the International Space Station present with neuro-ocular structural and/or functional changes, including optic disc edema, optic nerve sheath distension, globe flattening, choroidal folds, or hyperopic shifts. This spaceflight-associated neuro-ocular syndrome (SANS) represents a major risk to future exploration class human spaceflight missions, including Mars missions. Although the exact pathophysiology of SANS is unknown, evidence thus far suggests that an increase in intracranial pressure (ICP) relative to the upright position on Earth, which is due to the loss of hydrostatic pressure gradients in space, may play a leading role. This review focuses on brain physiology in the spaceflight environment, specifically on how spaceflight may affect ICP and related indicators of cranial compliance, potential factors related to the development of SANS, and findings from spaceflight as well as ground-based spaceflight analog research studies.
Identification of Cigarette Brands by Soft Independent Modeling of Class Analogy of Volatile Substances
Authors: Zelinkova Zuzana , Wenzl Thomas 
Published: April 2019

Abstract

Introduction
This study aimed to develop a method for discriminating cigarette brands based on the profiles of volatile components extracted from the tobacco fraction of the finished cigarettes to authenticate branded cigarettes of unknown origin.

Methods
An analytical method comprising direct thermal desorption coupled with gas chromatography-quadrupole time-of-flight mass spectrometry was developed for acquiring volatile profiles of cigarettes. About 290 samples of commercially available cigarettes were analyzed. Within this batch, 123 samples represented four popular cigarette brands. They were selected for in-depth characterization. Multivariate analysis was used to investigate the interrelations among volatile compounds of cigarettes and to identify characteristic markers for the cigarette discrimination. Supervised pattern recognition techniques were used for designing classification models.

Results
Principal component analysis covering all detected volatiles allowed the differentiation of cigarettes based on the brand. A number of 56 volatile components were identified as markers with high discrimination power. These compounds were used for establishing classification models. A method of soft independent modeling of class analogy developed for the four studied cigarette brands proved to be efficient in the classification of unknown cigarettes, with accuracy between 95.9% and 100%.

Conclusions
The data evaluation by soft independent modeling of class analogy was highly accurate in classification of unknown cigarettes with a low rate of false positives and false negatives. The developed models can be used for discrimination of genuine from non-genuine products with high level of probability.

Implications
Profiling of volatiles, which is commonly used for authentication of different food commodities, was applied for the characterization of cigarette tobacco for the purpose of authentication a cigarette brand. Volatile components with a high discrimination power were identified by means of multivariate statistical methods and used for establishing of a classification model. The classification model was able to discriminate genuine from non-genuine cigarettes with a high level of prediction accuracy. This model could be a powerful tool for tobacco control to judge the authenticity of cigarettes.
Tobacco Industry Promotions and Pricing After Tax Increases: An Analysis of Internal Industry Documents
Authors: Apollonio Dorie E , Glantz Stanton A 
Published: April 2019

Abstract

Background
Increasing tobacco taxes, and through them, prices, is an effective public health strategy to decrease tobacco use. The tobacco industry has developed multiple promotional strategies to undercut these effects; this study assessed promotions directed to wholesalers and retailers and manufacturer price changes that blunt the effects of tax and price increases.

Methods
We reviewed tobacco industry documents and contemporaneous research literature dated 1987 to 2016 to identify the nature, extent, and effectiveness of tobacco industry promotions and price changes used after state-level tobacco tax increases.

Results
Tobacco companies have created promotions to reduce the effectiveness of tobacco tax increases by encouraging established users to purchase tobacco in lower-tax jurisdictions and sometimes lowering manufacturer pricing to “undershift” smaller tax increases, so that tobacco prices increased by less than the amount of the tax.

Conclusions
Policymakers should address industry efforts to undercut an effective public health intervention through regulating minimum prices, limiting tobacco industry promotions, and by enacting tax increases that are large, immediate, and result in price increases.

Implications
Tobacco companies view excise tax increases on tobacco products as a critical business threat. To keep users from quitting or reducing tobacco use in response to tax increases, they have shifted manufacturer pricing and developed specific promotions that encourage customers to shop for lower-taxed products. Health authorities should address tobacco industry efforts to undercut the effects of taxes by regulating prices and promotions and passing large and immediate tax increases.
Louise Rosenblatt
Authors: Shook John R. 
Published: April 2019

Louise Rosenblatt (b. 1904–d. 2005) was a highly influential thinker in literary and critical theory, reading pedagogy, and education. She was professor of education at New York University from 1948 until 1972, and she continued to teach for many years at other universities. The impact of her writings extends to aesthetics, communication and media studies, and cultural studies. Her transactional theory of reading literature earned a permanent place among methodologies applied to the study of reader comprehension and improving the teaching of reading, from preschool to college-age years. She is most widely known for her “reader response” theory of literature. The process of reading is a dynamic transaction between the reader and the text, in which meaningful ideas arise for readers from their own thoughtful and creative interpretations. Her first book, Literature as Exploration, which was published in 1938, has gone through five editions and remains in print in the early 21st century. Her last book, Making Meaning with Texts: Selected Essays, was published in 2005 and contained selected essays from each decade of her career. Rosenblatt’s view of literary experience threw down a challenge to a dominant paradigm during the 1940s and 1950s, namely the New Criticism. New Criticism held that authentic meanings of a piece of creative writing—a novel, story, drama, poem, and so on—are already within the text itself, requiring attention to that somewhat concealed yet objective truth. Rosenblatt took the pragmatist approach, starting from the aesthetics of reading. As a member of the Conference on Methods in Philosophy and the Sciences at Columbia University during the 1930s, she studied John Dewey, Charles Peirce, and William James. During this time, she married the pragmatist philosopher Sidney Ratner. Rosenblatt applied her knowledge of pragmatism to the question of understanding creative writing. For pragmatism, all experiences are creative fusions of intersecting processes, some from within and some from without. Any comprehension of a text blends the reader’s particular approach for appreciating it together with the capacity of the text to provoke a variety of stimulating ideas. The emotional and the factual are rarely found in pure forms; only a gradual range from the affective to the cognitive can characterize lived experience. Understanding the process of reading in its fundamental experiential situation has been a revolutionary philosophical position, impacting both childhood education and literary theory. Rosenblatt’s work continues to inspire fresh academic research and curricular innovations.
To Experience or to Be Informed? Classical Conditioning Induces Nocebo Hyperalgesia even when Placebo Analgesia Is Verbally Suggested—Results of a Preliminary Study
Authors: Bajcar Elżbieta A , Wiercioch-Kuzianik Karolina , Adamczyk Wacław M , Bąbel Przemysław 
Published: April 2019

Abstract

Objective
To investigate whether direct experience (i.e., classical conditioning) or verbal suggestion is more important in inducing nocebo hyperalgesia, five groups (total sample size, N = 99) were studied: conditioning, congruent conditioning, incongruent conditioning, verbal suggestion, and control.

Methods
Participants in groups with conditioning experienced more intensive pain stimuli after presentation of a white circle. In the congruent conditioning group, suggestion that the circle would precede more intensive pain stimuli was additionally provided, whereas in the incongruent conditioning group, the opposite suggestion was used. Control and verbal suggestion groups received pain stimuli of one intensity; however, the latter received suggestion that a circle would precede pain stimuli of higher intensity.

Results
The nocebo effect was observed in all conditioning groups, regardless of the verbal suggestions used. Moreover, the experience of hyperalgesia was able to nullify the effect of the verbal suggestion of analgesia. Incongruence between verbal suggestion and pain experience produced expectancies that affected nocebo hyperalgesia.

Conclusions
The results of this preliminary study suggest that direct experience seems to be more important than verbal suggestion in inducing nocebo hyperalgesia.