bluuebunny/crossref_metadata_2025_split huggingface dataset:
Concordância intra e interobservador com relação ao sistema de classificação de Walch para artrose da articulação do ombro
Authors: Pajolli Lauro José Rocchetti , Carlini Marcelo Casciato , Ferrari Isabella , Matsunaga Fábio Teruo , Archetti Netto Nicola , Tamaoki Marcel Jun Sugawara 
Published: May 2018

Resumo
Objetivo Avaliar a concordância inter e intraobservador com relação ao sistema de classificação de Walch para artrose do ombro.
Materiais e Métodos Foram selecionadas tomografias computadorizadas da articulação do ombro de pacientes adultos entre 2012 e 2016, que foram classificadas por médicos com diferentes níveis de experiência em ortopedia. As imagens foram examinadas em três momentos distintos, e a análise foi avaliada pelo índice Kappa de Fleiss para verificar a concordância intra e interobservador.
Resultados O índice Kappa na concordância intraobservador variou entre 0,305 e 0,545. A concordância interobservador se mostrou muito baixa no fim das três avaliações (κ = 0,132).
Conclusão A concordância intraobservador com relação à classificação de Walch modificada mostrou-se variável, entre moderada e baixa. A concordância interobservador foi baixa.
Intermediate lakes of the Chulym and Kargat river valleys and their role in the evolution of the Lake Chany basin
Authors: Krivonogov S.K. , Gusev V.A. , Parkhomchuk E.V. , Zhilich S.V. 
Published: May 2018

Abstract
The Chulym and Kargat Rivers flow through chains of saucer-shaped depressions, which are swampy meadows or drainage lakes. In the past, all of them were lakes short-lived in different Holocene periods. These depressions accumulated a significant amount of the Chulym and Kargat runoff and thus influenced the water balance of Lake Chany. Our studies have reconstructed the history of these depressions. A model describing their filling with sediments is proposed. The lacustrine sediments penetrated by boreholes and pits are dated by the radiocarbon method at 6.3–2.0 cal. kyr BP. The data obtained explane the long existence of Lake Chany as a shallow basin and rise in its level at ~ 2 kyr BP.
MISSING THE POOREST IN RURAL AREAS? TARGETING LOW INCOME VOTERS IN MAYORAL ELECTIONS
Authors: Amick Joe 
Published: May 2018

AbstractThis article utilizes an original household survey of two regency-level elections in Indonesia to explore campaign targeting. It uses a list experiment to show that direct survey questions about accepting transfers from campaigns elicit honest responses from respondents in Indonesia. Although the relationship between income and whether a respondent accepted transfers from political campaigns decreases over the entire distribution of income, it increases initially, producing a curvilinear relationship between income and accepting transfers from campaigns. This article argues that the poorest voters face barriers to being targeted by campaigns. However, these barriers recede as they become relatively richer, at which point a negative relationship is found due to diminishing marginal utility of accepting these transfers. Finally, in-kind transfers, as opposed to cash transfers, target low-income voters more effectively.
Prenatal malnutrition and adult cognitive impairment: a natural experiment from the 1959–1961 Chinese famine
Authors: He Ping , Liu Li , Salas J. M. Ian , Guo Chao , Cheng Yunfei , Chen Gong , Zheng Xiaoying 
Published: May 2018

AbstractThe current measures of cognitive functioning in adulthood do not indicate a long-term association with prenatal exposure to the Dutch famine. However, whether such association emerges in China is poorly understood. We aimed to investigate the potential effect of prenatal exposure to the 1959–1961 Chinese famine on adult cognitive impairment. We obtained data from the Second National Sample Survey on Disability implemented in thirty-one provinces in 2006, and restricted our analysis to 387 093 individuals born in 1956–1965. Cognitive impairment was defined as intelligence quotient (IQ) score under 70 and IQ of adults was evaluated by the Wechsler Adult Intelligence Scale – China Revision. Famine severity was defined as excess death rate. The famine impact on adult cognitive impairment was estimated by difference-in-difference models, established by examining the variations of famine exposure across birth cohorts. Results show that compared with adults born in 1956–1958, those who were exposed to Chinese famine during gestation (born in 1959–1961) were at greater risk of cognitive impairment in the total sample. Stratified analyses showed that this effect was evident in males and females, but only in rural, not in urban areas. In conclusion, prenatal exposure to famine had an enduring deleterious effect on risk of cognitive impairment in rural adults.
Recruitment advertising for Antarctic personnel: between adventure and routine
Authors: Nielsen Hanne E. F. , Jaksic Cyril 
Published: May 2018

ABSTRACTThis paper examines how Antarctica has been depicted in recruitment material, and compares the expectations set up in the advertising imagery with the reality of expeditioners’ experiences. Textual analyses of advertisements and job descriptions are used to reveal dominant themes, including the trope of extremity, while interviews with those who have spent time on the ice provide reflections on the actual challenges encountered when working in Antarctica, such as boredom. Much of the popular discourse around Antarctica continues to centre on the Heroic Era (1895–1922), a time of exploration typified by men pitting themselves against nature and striding out into unchartered expanses of ice. Although modern day life on Antarctic stations differs markedly from the extreme conditions experienced by early explorers, the continent continues to be associated with notions of toughness and extremity. We argue that in some cases, advertisements may actually target the wrong audience. This has important implications for how an Antarctic station as a workplace is conceptualised, and then experienced by those who head south, with potential detrimental effects.
Revisiting Labor Activism in Iran: Some Notes on the Vatan Factory Strike in 1931
Authors: Afacan Serhan 
Published: May 2018

AbstractThis article discusses the discursive practices of Iranian workers between 1921 and 1941, with a particular focus on the Vatan Factory strike, which took place in Isfahan in May 1931. Initially, the article provides a brief background on the industrialization process of the era for a better contextualization of the factory and the strike under study. Then, it discusses Iranian workers’ self-perceptions and the discursive practices and strategies they used in their engagement with the ruling classes, by utilizing the petitions they sent to various authorities. And finally, it elaborates on the Vatan Factory strike in order to gain a better understanding of workers’ self-representations and the changing dynamics between them and the official authorities. In the main, this article suggests that notwithstanding the confrontational nature of this particular labor action, striking workers’ discursive engagement was in conformity with the developing labor discourse at large.
The New Speech Politics
Authors: Raine Barnaby 
Published: May 2018

AbstractIn engaging the popularity of “free speech” as a conservative battle cry in recent years, this article poses two questions: What particular conception of that demand does the right defend, and how should the left respond? Finding that conservatives have moved from a “negative” to a “positive” understanding of this freedom selectively applied, this article presents the consistent application of that positive understanding of free speech as a challenge to concentrations of power that conspire to privilege some speech over others. Turning to recent attempts by graduate students on North American campuses to unionize, the article attempts to ground a reading of union rights as free speech rights. Free speech is presented as a useful frame through which to think about political struggles more generally, though the article cautions against treating free speech as an invariably desirable principle.
Populism is Not in the Air (but maybe it should be)
Authors: Semán Ernesto 
Published: May 2018

AbstractAnalogies between the rise of Donald Trump in the United States and populist leaders in Latin America are an emblematic case of misleading comparative analysis. They hide the ideological core of Trumpism, which undermines collective action and its emblematic institution, organized labor, dismantling the protections it attained during the twentieth century. In particular, references to Argentina's Juan Perón obscure Trump's emphasis on individual economic freedom, property rights, and the naturalization of inequality, values engrained in American political thinking. They also distort the populist legacies in Latin America. Populist leaders in the postwar years challenged liberal democratic thinking by posing that social rights and collective prerogatives for excluded groups should prevail over individual citizenship in order to expand political democracy. That legacy is not only alien to Trumpism; it might also be the main weapon to fight against it.
Evaluation of Cotton Responses to Fomesafen-Based Treatments Applied Preemergence
Authors: Li Xiao , Grey Timothy , Vencill William , Freeman James , Price Katilyn , Cutts George , Price Andrew 
Published: May 2018

AbstractFomesafen provides effective control of glyphosate-resistant Palmer amaranth in cotton. However, cotton seedlings can be injured when fomesafen is applied PRE. Therefore, greenhouse and field experiments were conducted at Athens, GA, and at six locations in Alabama and Georgia in 2013 and 2016 to evaluate cotton growth and yield response to fomesafen applied PRE at 70, 140, 280, 560, 1,120, or 2,240 g ai ha−1, and in combination with pendimethalin, diuron, acetochlor, and fluridone at 1×label rates. Greenhouse bioassays indicated that fomesafen reduced cotton height and dry weight with increasing rate in Cecil sandy loam and Tifton loamy sand but not in Greenville sandy clay loam––possibly as a result of this soil’s higher organic matter (OM) and clay content. Fomesafen applied at 2,240 g ai ha−1reduced cotton stand by as much as 83% compared to the nontreated check (NTC) at all field locations except Alabama’s Macon and Baldwin counties, and 1,120 g ai ha−1reduced cotton stand only at Pulaski County, GA, by 52%. Cotton height was reduced by the two highest rates of fomesafen at all locations except Clarke County, GA, and Baldwin County, AL. Injury data indicated more visual injury followed increasing fomesafen rates, and high-rate treatments produced more injury in sandier soils. Cotton yield was unaffected by herbicide treatments at any location, except for the 1,120 g ai ha−1rate at Pulaski County (49% yield loss compared to NTC), 2,240 g ai ha−1at Pulaski County (72% yield loss), and Tift County (29% yield loss). These data indicated cotton yield should not be negatively affected by fomesafen applied PRE alone within label rates or in combination with pendimethalin, diuron, acetochlor, and fluridone at 1×label rates, although some visual injury, or stand or height reduction may occur early in the growing season.
Transcriptome analysis in whole blood reveals increased microbial diversity in schizophrenia
Authors: Olde Loohuis Loes M. , Mangul Serghei , Ori Anil P. S. , Jospin Guillaume , Koslicki David , Yang Harry Taegyun , Wu Timothy , Boks Marco P. , Lomen-Hoerth Catherine , Wiedau-Pazos Martina , Cantor Rita M. , de Vos Willem M. , Kahn René S. , Eskin Eleazar , Ophoff Roel A. 
Published: May 2018

AbstractThe role of the human microbiome in health and disease is increasingly appreciated. We studied the composition of microbial communities present in blood across 192 individuals, including healthy controls and patients with three disorders affecting the brain: schizophrenia, amyotrophic lateral sclerosis, and bipolar disorder. By using high-quality unmapped RNA sequencing reads as candidate microbial reads, we performed profiling of microbial transcripts detected in whole blood. We were able to detect a wide range of bacterial and archaeal phyla in blood. Interestingly, we observed an increased microbial diversity in schizophrenia patients compared to the three other groups. We replicated this finding in an independent schizophrenia case–control cohort. This increased diversity is inversely correlated with estimated cell abundance of a subpopulation of CD8+ memory T cells in healthy controls, supporting a link between microbial products found in blood, immunity and schizophrenia.
Iron overload promotes mitochondrial fragmentation in mesenchymal stromal cells from myelodysplastic syndrome patients through activation of the AMPK/MFF/Drp1 pathway
Authors: Zheng Qingqing , Zhao Youshan , Guo Juan , Zhao Sida , Fei Chengming , Xiao Chao , Wu Dong , Wu Lingyun , Li Xiao , Chang Chunkang 
Published: May 2018

AbstractIron overload (IO) has been reported to contribute to mesenchymal stromal cell (MSC) damage, but the precise mechanism has yet to be clearly elucidated. In this study, we found that IO increased cell apoptosis and lowered cell viability in MSCs, accompanied by extensive mitochondrial fragmentation and autophagy enhancement. All these effects were reactive oxygen species (ROS) dependent. In MSCs with IO, the ATP concentrations were significantly reduced due to high ROS levels and low electron respiratory chain complex (ETC) II/III activity. Reduced ATP phosphorylated AMP-activated protein kinase (AMPK). Activation of AMPK kinase complexes triggered mitochondrial fission. Moreover, gene knockout of AMPK via CRISPR/Cas9 reduced cell apoptosis, enhanced cell viability and attenuated mitochondrial fragmentation and autophagy caused by IO in MSCs. Further, AMPK-induced mitochondrial fragmentation of MSCs with IO was mediated via phosphorylation of mitochondrial fission factor (MFF), a mitochondrial outer-membrane receptor for the GTPase dynamin-related protein 1 (Drp1). Gene knockdown of MFF reversed AMPK-induced mitochondrial fragmentation in MSCs with IO. In addition, MSCs from IO patients with myelodysplastic syndrome (MDS) showed increased cell apoptosis, decreased cell viability, higher ROS levels, lower ATP concentrations and increased mitochondrial fragmentation compared with MSCs from non-IO patients. In addition, iron chelation or antioxidant weakened the activity of the AMPK/MFF/Drp1 pathway in MDS-MSCs with IO from several patients, accompanied by attenuation of mitochondrial fragmentation and autophagy. Taken together, the AMPK/MFF/Drp1 pathway has an important role in the damage to MDS-MSCs caused by IO.
HOXB9 promotes endometrial cancer progression by targeting E2F3
Authors: Wan Junhu , Liu Hongyang , Feng Quanling , Liu Jun , Ming Liang 
Published: May 2018

AbstractHOXB9, as a HOX family transcription factor, playing a significant role in embryonic development and cancer progression. However, the function of HOXB9 and its precise mechanism in regulating endometrial cancer progression remains unknown. Here, we demonstrated that the expression of HOXB9 was increased in endometrial cancer, and associated with histological grade and lymph node metastasis. In addition, elevated HOXB9 predicts a poor prognosis in endometrial cancer patients. Interestingly, bioinformatics analysis of TCGA cancer database showed that HOXB9 expression is positively correlated with E2F3 expression. Moreover, HOXB9 promoted E2F3 expression by directly targeting to its promoter. Furthermore, we found that knocking down E2F3 abolished the ability of HOXB9 in enhancing cell migration. Taken together, for the first, we demonstrated the function and mechanism of HOXB9 in regulating endometrial cancer progression, and indicated HOXB9 may be a novel prognostic marker of endometrial cancer.
Nucleolar TRF2 attenuated nucleolus stress-induced HCC cell-cycle arrest by altering rRNA synthesis
Authors: Yuan Fuwen , Xu Chenzhong , Li Guodong , Tong Tanjun 
Published: May 2018

AbstractThe nucleolus is an important organelle that is responsible for the biogenesis of ribosome RNA (rRNA) and ribosomal subunits assembly. It is also deemed to be the center of metabolic control, considering the critical role of ribosomes in protein translation. Perturbations of rRNA synthesis are closely related to cell proliferation and tumor progression. Telomeric repeat-binding factor 2 (TRF2) is a member of shelterin complex that is responsible for telomere DNA protection. Interestingly, it was recently reported to localize in the nucleolus of human cells in a cell-cycle-dependent manner, while the underlying mechanism and its role on the nucleolus remained unclear. In this study, we found that nucleolar and coiled-body phosphoprotein 1 (NOLC1), a nucleolar protein that is responsible for the nucleolus construction and rRNA synthesis, interacted with TRF2 and mediated the shuttle of TRF2 between the nucleolus and nucleus. Abating the expression of NOLC1 decreased the nucleolar-resident TRF2. Besides, the nucleolar TRF2 could bind rDNA and promoted rRNA transcription. Furthermore, in hepatocellular carcinoma (HCC) cell lines HepG2 and SMMC7721, TRF2 overexpression participated in the nucleolus stress-induced rRNA inhibition and cell-cycle arrest.
Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis
Authors: Sathe Mugdha , Muthukrishnan Gayatri , Rae James , Disanza Andrea , Thattai Mukund , Scita Giorgio , Parton Robert G. , Mayor Satyajit 
Published: May 2018

AbstractUsing real-time TIRF microscopy imaging, we identify sites of clathrin and dynamin-independent CLIC/GEEC (CG) endocytic vesicle formation. This allows spatio-temporal localisation of known molecules affecting CG endocytosis; GBF1 (a GEF for ARF1), ARF1 and CDC42 which appear sequentially over 60 s, preceding scission. In an RNAi screen for BAR domain proteins affecting CG endocytosis, IRSp53 and PICK1, known interactors of CDC42 and ARF1, respectively, were selected. Removal of IRSp53, a negative curvature sensing protein, abolishes CG endocytosis. Furthermore, the identification of ARP2/3 complex at CG endocytic sites, maintained in an inactive state reveals a function for PICK1, an ARP2/3 inhibitor. The spatio-temporal sequence of the arrival and disappearance of the molecules suggest a mechanism for a clathrin and dynamin-independent endocytic process. Coincident with the loss of PICK1 by GBF1-activated ARF1, CDC42 recruitment leads to the activation of IRSp53 and the ARP2/3 complex, resulting in a burst of F-actin polymerisation potentially powering scission.
Biosynthetic pathway for furanosteroid demethoxyviridin and identification of an unusual pregnane side-chain cleavage
Authors: Wang Gao-Qian , Chen Guo-Dong , Qin Sheng-Ying , Hu Dan , Awakawa Takayoshi , Li Shao-Yang , Lv Jian-Ming , Wang Chuan-Xi , Yao Xin-Sheng , Abe Ikuro , Gao Hao 
Published: May 2018

AbstractFuranosteroids, represented by wortmannin, viridin, and demethoxyviridin, are a special group of fungal-derived, highly oxygenated steroids featured by an extra furan ring. They are well-known nanomolar-potency inhibitors of phosphatidylinositol 3-kinase and widely used in biological studies. Despite their importance, the biosyntheses of these molecules are poorly understood. Here, we report the identification of the biosynthetic gene cluster for demethoxyviridin, consisting of 19 genes, and among them 15 biosynthetic genes, including six cytochrome P450 monooxygenase genes, are deleted. As a result, 14 biosynthetic intermediates are isolated, and the biosynthetic pathway for demethoxyviridin is elucidated. Notably, the pregnane side-chain cleavage requires three enzymes: flavin-dependent Baeyer-Villiger monooxygenase, esterase, and dehydrogenase, in sharp contrast to the single cytochrome P450-mediated process in mammalian cells. Structure–activity analyses of these obtained biosynthetic intermediates reveal that the 3-keto group, the C1β–OH, and the aromatic ring C are important for the inhibition of phosphatidylinositol 3-kinase.
qSR: a quantitative super-resolution analysis tool reveals the cell-cycle dependent organization of RNA Polymerase I in live human cells
Authors: Andrews J. O. , Conway W. , Cho W -K. , Narayanan A. , Spille J -H. , Jayanth N. , Inoue T. , Mullen S. , Thaler J. , Cissé I. I. 
Published: May 2018

AbstractWe present qSR, an analytical tool for the quantitative analysis of single molecule based super-resolution data. The software is created as an open-source platform integrating multiple algorithms for rigorous spatial and temporal characterizations of protein clusters in super-resolution data of living cells. First, we illustrate qSR using a sample live cell data of RNA Polymerase II (Pol II) as an example of highly dynamic sub-diffractive clusters. Then we utilize qSR to investigate the organization and dynamics of endogenous RNA Polymerase I (Pol I) in live human cells, throughout the cell cycle. Our analysis reveals a previously uncharacterized transient clustering of Pol I. Both stable and transient populations of Pol I clusters co-exist in individual living cells, and their relative fraction vary during cell cycle, in a manner correlating with global gene expression. Thus, qSR serves to facilitate the study of protein organization and dynamics with very high spatial and temporal resolutions directly in live cell.
Heritability of Sleep EEG Topography in Adolescence: Results from a Longitudinal Twin Study
Authors: Markovic Andjela , Achermann Peter , Rusterholz Thomas , Tarokh Leila 
Published: May 2018

AbstractThe topographic distribution of sleep EEG power is a reflection of brain structure and function. The goal of this study was to examine the degree to which genes contribute to sleep EEG topography during adolescence, a period of brain restructuring and maturation. We recorded high-density sleep EEG in monozygotic (MZ; n = 28) and dizygotic (DZ; n = 22) adolescent twins (mean age = 13.2 ± 1.1 years) at two time points 6 months apart. The topographic distribution of normalized sleep EEG power was examined for the frequency bands delta (1–4.6 Hz) to gamma 2 (34.2–44 Hz) during NREM and REM sleep. We found highest heritability values in the beta band for NREM and REM sleep (0.44 ≤ h2 ≤ 0.57), while environmental factors shared amongst twin siblings accounted for the variance in the delta to sigma bands (0.59 ≤ c2 ≤ 0.83). Given that both genetic and environmental factors are reflected in sleep EEG topography, our results suggest that topography may provide a rich metric by which to understand brain function. Furthermore, the frequency specific parsing of the influence of genetic from environmental factors on topography suggests functionally distinct networks and reveals the mechanisms that shape these networks.