bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of wheat gluten–soy protein ratios and moisture levels on high‐moisture extruded meat analogues for burger patties
Authors: Dubey Aishwary , Kumar Arun , Singh Narpinder 
Published: November 2024

AbstractIn response to the growing demand for plant‐based meat alternatives, this study explores the impact of incorporating wheat gluten (WG) into soy protein isolate (SPI) on both the proximate and functional properties of protein blends. The research also examines the effects of high moisture extrusion processing, varying feed moisture levels (60%, 65%, and 70%), and WG‐SPI blends on extruder response, as well as the textural, rheological, and solubility characteristics of the resulting extruded meat analogues. Moreover, the prime objective was to gain insights into the impact of using HMMA made at different ratios and feed moisture levels on plant‐based burgers. As the WG incorporation level increased in SPI, the RVA viscosity, water absorption, and oil absorption capacities, and foaming stability exhibited a decrease while foaming capacity increased. As feed moisture and WG incorporation levels in SPI increased, the system parameters, rheological, and textural parameters of high moisture extruded meat analogs decreased. WG25‐SPI75 showed the highest degree of texturization or sulfide bonds, and its extruded meat analogues at 60% feed moisture level burger patties resemble a similar textural to a chicken burger patty. This study is pivotal in understanding how wheat gluten in SPIs and feeding moisture level influence the textural and rheology properties of HMMA.
Characteristics of whey protein concentrate/egg white protein composite film modified by transglutaminase and its application on cherry tomatoes
Authors: Jiang Shujuan , Zhang Jiaxin , Zhang Mengyuan , Qian Fang , Mu Guangqing 
Published: November 2024

AbstractIn order to obtain food packaging film with better performance, whey protein concentrate (WPC) and egg white protein (EWP) were used as film‐forming substrates, and its film properties were modified by transglutaminase (TG). Then the effect of TG on the mechanical, physical, barrier, and microstructural properties of the WPC/EWP composite biodegradable film was investigated, and its preliminary application potential was explored. Compared to WPC and EWP films, WPC/EWP composite film had higher transmittance, tensile strength (TS), and thermal stability. Fluorescence results showed that the film experienced fluorescence quenching after TG treatment. Fourier transform infrared and x‐ray diffraction results showed that WPC and EWP had good compatibility in the biodegradable film, the hydrogen bond interaction of film was increased due to TG, resulting in an increase in TS. Meanwhile, the water vapor permeability and contact angle of WPC/EWP film treated with TG at 5 U/g protein increased by 28% and 76.1%, respectively. Besides, the WPC/EWP biodegradable film modified by TG (TG‐W/E) was applied as a coating film on cherry tomatoes, effectively reducing the weight loss rate during storage from 14.2% to 10.8%. Furthermore, indexes, such as solid content, spoilage rate, hardness, pH, and lycopene, showed that the film had a good preservation effect on cherry tomatoes. To conclude, the appropriate addition of TG has a positive effect on the film properties of the WPC/EWP biodegradable film, which is beneficial to the development and utilization of protein‐based film. WPC/EWP biodegradable film modified by TG has a great application prospect in extending the shelf life of fruit and vegetable.
Switching to reusable operating theatre equipment: lessons learnt from sterile light handle projects in two Australian hospitals
Authors: Chen Stanley , McAlister Scott , Colagiuri Philomena , Pickles Kristen , Barratt Alexandra L. 
Published: November 2024

AbstractBackgroundReplacing single‐use operating theatre equipment with reusables might be one strategy for reducing the carbon footprint of operating theatres. However, in Australia, where the energy mix is predominantly fossil‐fuel‐based, the re‐sterilization of reusables may increase the carbon footprint. We analyzed the financial and environmental impacts of introducing reusable operating theatre light handles in two NSW hospitals.MethodsThe effects on cost, waste, and carbon footprint of replacing disposable light handle covers with reusable handles in each hospital were analyzed over 12 months using procurement, waste and sterilization data, and life cycle assessment.ResultsEnergy requirement for sterilization of reusable handles, increasing alongside weight of the handle, resulted in higher carbon footprint than using disposable covers. At one hospital, using a heavy handle increased carbon emissions sixfold, while the cost of handle sterilization exceeded the cost of disposable covers, resulting in 11% higher cost per use. At the other hospital, using a lighter handle increased carbon emissions by 40% per use, while sterilization cost was less than the cost of disposable covers, resulting in 14.8% lower cost per use. Scenario modelling indicated that sterilizing handles as part of a hollowware set rather than as individual items would significantly reduce cost and carbon footprint. At both hospitals, associated clinical waste was essentially eliminated.ConclusionJudicious replacement of disposable covers with lightweight yet durable reusable handles can reduce costs, but increases carbon footprint in the current Australian energy context. Adopting predominantly renewable energy and more efficient sterilization practice would mitigate this.
Paediatric bone marrow mesenchymal stem cells support acute myeloid leukaemia cell survival and enhance chemoresistance via contact‐independent mechanism
Authors: Laing Alison , Elmarghany Ahmed , Alghaith Arwa A. , Gouma Aya , Stevens Thomas , Winton Alexander , Cassels Jennifer , Clarke Cassie J. , Schwab Claire , Harrison Christine J. , Gibson Brenda , Keeshan Karen 
Published: November 2024

SummaryChildren diagnosed with acute myeloid leukaemia (paediatric AML [pAML]) have limited treatment options and relapse rates due to chemoresistance and refractory disease are over 30%. Current treatment is cytotoxic and in itself has long‐lasting harsh side effects. New, less toxic treatments are needed. The bone marrow microenvironment provides chemoprotection to leukaemic cells through cell communication and interaction with mesenchymal stem cells (MSCs), but this is not well defined in pAML. Using primary patient material, we identify a cell contact‐independent mechanism of MSC‐mediated chemoprotection involving extrinsic soluble factors that is abrogated through inhibition of the JAK/STAT and ERK pathways.
Long‐term tracking of haematopoietic clonal dynamics and mutations in non‐human primate undergoing transplantation of lentivirally barcoded haematopoietic stem and progenitor cells
Authors: Hosuru Rohan V. , Yang Jack , Zhou Yifan , Gin Ashley , Hayal Taha B. , Hong So Gun , Dunbar Cynthia E. , Wu Chuanfeng 
Published: November 2024

SummaryHaematopoietic stem and progenitor cell (HSPC) autologous gene therapies are promising treatment for a variety of blood disorders. Investigation of the long‐term HSPC clonal dynamics and other measures of safety and durability following lentiviral‐mediated gene therapies in predictive models are crucial for assessing risks and benefits in order to inform decisions regarding wider utilization. We established an autologous lentivirally barcoded HSPC transplantation model in rhesus macaque (RM), a model offering insights into haematopoiesis and gene therapies with direct relevance to human. Healthy young adult RMs underwent total body irradiation, followed by transplantation of autologous HSPCs transduced with a lentiviral vector containing a diverse genetic barcode library, uniquely labelling individual HSPCs and their progeny. With up to 131 months of follow‐up, we now report quantitative clonal dynamics, characterizing the number, diversity, stability and lineage bias of hundreds of thousands of HSPC clones tracked in five RMs. We documented long‐term stable and multi‐lineage output from a highly polyclonal pool of HSPCs. Clonal succession after stable haematopoietic reconstitution was minimal. There was no evidence for accelerated acquisition of acquired somatic mutations following autologous lentivirally transduced HSPC transplantation. Our results provide relevant insights into long‐term HSPC behaviours in vivo following transplantation and gene therapies.
The opposite roles of injustice and cruelty in the internalization of a devaluation: The humiliation paradox revisited
Authors: Gonzalez‐Puerto Jose A. , Fernández Saulo 
Published: November 2024

AbstractCruelty and its link to injustice in contexts of humiliation have not received to date due attention from experimental psychosocial research. Aiming at filling this gap, this paper presents three studies with increasing degrees of experimental control (Ntotal = 1098) that show a dual opponent‐process response to being targeted by potentially humiliating actions: while targets appraising more injustice internalize less the devaluation underlying the humiliation experience (thus partially dissolving the so‐called “paradox of humiliation”, Fernández et al., 2015, Personality and Social Psychology Bulletin, 41, 976), targets appraising more cruelty internalize more a devalued self‐view and feel more humiliated. The fine balance between these two closely connected but distinct appraisals is key to understand the internal/subjective experience of targets: seeing themselves mainly as victims of injustice or cruelty will prevent or favour, respectively, their internalization of the devaluation and their feeling humiliated. This opposite pattern also impacts victims' reaction: Both appraisals relate to aggressive responses via anger but while appraising cruelty also paradoxically leads to powerless inertia, appraising injustice (including importantly the injustice of cruelty itself) leads to less powerlessness and more assertive agency. The theoretical and applied implications of approaching the victims of humiliation as victims of both an injustice and a cruelty are discussed.
Antisense oligonucleotides as a precision therapy for developmental and epileptic encephalopathies
Authors: Quilón Paloma García , Volpedo Greta , Cappato Serena , Ferrera Loretta , Zara Federico , Bocciardi Renata , Riva Antonella , Striano Pasquale 
Published: November 2024

AbstractDevelopmental and epileptic encephalopathies (DEEs) comprise a complex spectrum of neurological disorders characterized by neurodevelopmental delay and early‐onset seizures primarily caused by diverse genetic mutations. Traditional treatments have largely been symptomatic, focusing on seizure control without addressing the underlying genetic causes. The advent of gene therapy, particularly through antisense oligonucleotides (ASOs), offers a promising avenue toward targeted therapeutic interventions. ASOs by virtue of their ability to modulate gene expression at the mRNA level represent a sophisticated approach to counteract the effects of pathogenic mutations. This review delves into the recent advancements in ASO technology, highlighting its application in preclinical and clinical settings for DEEs. We present evidence of the efficacy of ASOs in ameliorating disease phenotypes in vitro and in vivo, alongside promising outcomes from ongoing clinical trials. The therapeutic landscape for DEEs is on the cusp of significant transformation, underscored by the potential of ASOs to offer precise, personalized, treatments that extend beyond symptomatic relief to potentially rectify the genetic underpinnings of these disorders.
Static and Dynamic Functional Network Connectivity in Parkinson's Disease Patients With Postural Instability and Gait Disorder
Authors: Shen Bo , Yao Qun , Zhang Yixuan , Jiang Yinyin , Wang Yaxi , Jiang Xu , Zhao Yang , Zhang Haiying , Dong Shuangshuang , Li Dongfeng , Chen Yaning , Pan Yang , Yan Jun , Han Feng , Li Shengrong , Zhu Qi , Zhang Daoqiang , Zhang Li , Wu Yun‐cheng 
Published: November 2024

ABSTRACTAimsThe exact cause of the parkinsonism gait remains uncertain. We first focus on understanding the underlying neurological reasons for these symptoms through the examination of both static functional network connectivity (SFNC) and dynamic functional network connectivity (DFNC).MethodsWe recruited 64 postural instability and gait disorder‐dominated Parkinson's disease (PIGD‐PD) patients, 31 non‐PIGD‐PD (nPIGD‐PD) patients, and 54 healthy controls (HC) from Nanjing Brain Hospital. The GIFT software identified five distinct independent components: the basal ganglia (BG), cerebellum (CB), sensory networks (SMN), default mode network (DMN), and central executive network (CEN). We conducted a comparison between the SFNC and DFNC of the five networks and analyzed their correlations with postural instability and gait disorder (PIGD) symptoms.ResultsCompared with nPIGD‐PD patients, the PIGD‐PD patients demonstrated reduced connectivity between CEN and DMN while spending less mean dwell time (MDT) in state 4. This is characterized by strong connections. Compared with HC, PIGD‐PD patients exhibited enhanced connectivity in the SFNC between CB and CEN, as well as the network between CB and DMN. Patients with PIGD‐PD spent more MDT in state 1, which is characterized by few connections, and less MDT in state 4. In state 3, there was an increase in the functional connectivity between the CB and DMN in patients with PIGD‐PD. The nPIGD patients showed increased SFNC connectivity between CB and DMN compared to HC. These patients spent more MDT in state 1 and less in state 4. The MDT and fractional windows of state 2 showed a positive link with PIGD scores.ConclusionPatients with PIGD‐PD exhibit a higher likelihood of experiencing reduced brain connectivity and impaired information processing. The enhanced connection between the cerebellum and DMN networks is considered a type of dynamic compensation.
Predicting tumour resistance to paclitaxel and carboplatin utilising genome‐wide screening in haploid human embryonic stem cells
Authors: Nissenbaum Jonathan , Segal Emanuel , Philip Hagit , Cashman Rivki , Golan‐Lev Tamar , Reubinoff Benjamin E. , Turjeman Adi , Yanuka Ofra , Lezmi Elyad , Kopper Oded , Benvenisty Nissim 
Published: November 2024

AbstractTaxanes and platinum molecules, specifically paclitaxel and carboplatin, are widely used anticancer drugs that induce cell death and serve as first‐line chemotherapy for various cancer types. Despite the efficient effect of both drugs on cancer cell proliferation, many tumours have innate resistance against paclitaxel and carboplatin, which leads to inefficient treatment and poor survival rates. Haploid human embryonic stem cells (hESCs) are a novel and robust platform for genetic screening. To gain a comprehensive view of genes that affect or regulate paclitaxel and carboplatin resistance, genome‐wide loss‐of‐function screens in haploid hESCs were performed. Both paclitaxel and carboplatin screens have yielded selected plausible gene lists and pathways relevant to resistance prediction. The effects of mutations in selected genes on the resistance to the drugs were demonstrated. Based on the results, an algorithm that can predict resistance to paclitaxel or carboplatin was developed. Applying the algorithm to the DNA mutation profile of patients' tumours enabled the separation of sensitive versus resistant patients, thus, providing a prediction tool. As the anticancer drugs arsenal can offer alternatives in case of resistance to either paclitaxel or carboplatin, an early prediction can provide a significant advantage and should improve treatment. The algorithm assists this unmet need and helps predict whether a patient will respond to the treatment and may have an immediate clinically actionable application.
Developing guidelines on EFT for same‐sex/gender relationships: Recommendations from a Delphi study
Authors: Edwards Caitlin , Wittenborn Andrea K. , Allan Robert 
Published: November 2024

AbstractEmotionally focused therapy (EFT) is one of few empirically supported treatments for relationship distress. While evidence‐based approaches are critical for ensuring safe and effective treatment, EFT has not been adapted for use with same‐sex/same‐gender (SS/SG) relationships. This study used the Delphi method to generate consensus on treatment guidelines for using EFT with SS/SG relationships. Forty therapists with clinical expertise in EFT for SS/SG relationships were recruited. Data were collected in three phases. In phase one, participants responded to open‐ended questions regarding how EFT should be adapted for SS/SG relationships. Phases two and three involved participants rating the importance of the recommended guidelines. Data were analyzed using thematic analysis and descriptive statistics. The final recommendations included 49 guidelines on EFT for SS/SG relationships. The data reflected modifications to therapists' foundational knowledge and development, practice set up and orientation, and the three stages and nine steps of EFT.
Associations of comorbid headache disorders and depression with leisure‐time physical activity among 14,088 adults in The Brazilian Longitudinal Study of Adult Health
Authors: de Oliveira Arão Belitardo , Peres Mario Fernando Prieto , Mercante Juliane Prieto Peres , Brunoni André R. , Wang Yuan‐Pang , Molina Maria del Carmen B. , Uchiyama Lucas K. , Lotufo Paulo A. , Benseñor Isabela M. , Goulart Alessandra C. 
Published: November 2024

AbstractBackgroundWhile headache disorders are linked to low physical activity levels, the impact of depression on this relationship is unclear.ObjectiveTo assess how single and comorbid diagnoses of migraine and tension‐type headache (TTH) interact with depression and leisure‐time physical activity (LTPA) levels in The Brazilian Longitudinal Study of Adult Health (ELSA‐Brasil).MethodsIn this cross‐sectional analysis based on the ELSA‐Brasil baseline data, the relationship of migraine, TTH (both assessed with the International Classification of Headache Disorders, Second Edition), and depression (assessed with the Clinical Interview Schedule–Revised) with LTPA levels (International Physical Activity Questionnaire) was investigated by employing linear regression models. Models were adjusted for sociodemographic, lifestyle, and clinical covariates, and interaction terms were created to examine additive effects of comorbid conditions.ResultsAmong 14,088 participants, 54.4% (7668/14,088) were female, prevalence rates were: TTH = 39.6% (5573/14,088), migraine = 27.0% (3806/14,088), depression = 0.7% (94/14,088), depression + TTH = 1.1% (148/14,088), and depression + migraine = 2.5% (356/14,088). The mean (standard deviation) LTPA levels across the groups were: no headache + no depression = 148.7 (183.0) min/week, TTH = 133.5 (170.1) min/week, migraine = 110.3 (154.8) min/week, depression = 76.5 (146.3) min/week, depression + TTH = 84.5 (127.7) min/week, and depression + migraine = 64.3 (123.2) min/week. Negative associations were found for depression (β = −55.1, 95% confidence interval [CI] −93.6 to −17.0; p = 0.005), migraine (β = −24.7, 95% CI −33.2 to −15.4; p < 0.001), and TTH (β = −15.5, 95% CI −23.1 to −7.6; p < 0.001) with LTPA. No interaction effect was observed for depression + TTH (β = 36.0, 95% CI −12.6 to 84.6; p = 0.147) and depression + migraine (β = 31.7, 95% CI −11.3 to 74.7; p = 0.149), indicating no additive effect of comorbid conditions on LTPA levels. After adjusting for headache attack frequency, only depression + migraine remained negatively associated with LTPA (β = −38.7, 95% CI −71.6 to −5.8; p = 0.021).ConclusionsHeadache disorders and depression were independently and inversely associated with LTPA, with the strongest effects seen in depression alone or comorbid with migraine.
Efficacy of durvalumab plus tremelimumab treatment for unresectable hepatocellular carcinoma in immunotherapy era clinical practice
Authors: Hiraoka Atsushi , Tada Toshifumi , Hirooka Masashi , Kariyama Kazuya , Tani Joji , Atsukawa Masanori , Takaguchi Koichi , Itobayashi Ei , Fukunishi Shinya , Tsuji Kunihiko , Ishikawa Toru , Tajiri Kazuto , Ohama Hideko , Toyoda Hidenori , Ogawa Chikara , Nishimura Takashi , Hatanaka Takeshi , Kakizaki Satoru , Kawata Kazuhito , Naganuma Atsushi , Kosaka Hisashi , Matono Tomomitsu , Kuroda Hidekatsu , Yata Yutaka , Nishikawa Hiroki , Imai Michitaka , Aoki Tomoko , Ochi Hironori , Tada Fujimasa , Nakamura Shinichiro , Nakamura Yoshiko , Nouso Kazuhiro , Morishita Asahiro , Itokawa Norio , Okubo Tomomi , Arai Taeang , Tsutsui Akemi , Nagano Takuya , Tanaka Kazunari , Tanaka Hironori , Koshiyama Yuichi , Kanayama Yuki , Noritake Hidenao , Enomoto Hirayuki , Kaibori Masaki , Hiasa Yoichi , Kudo Masatoshi , Kumada Takashi ,   RELPEC Group and HCC 48 Group
Published: November 2024

AbstractAimSince the development of tremelimumab plus durvalumab (Dur/Tre) for unresectable hepatocellular carcinoma (uHCC), it has been used as not only an initial but also later line treatment in clinical practice. This study aimed to elucidate clinical prognostic factors for progression‐free survival (PFS) in Dur/Tre treatment cases.MethodsEnrolled were 183 uHCC patients treated with Dur/Tre from 2023 to May 2024 (median age, 74 years; male patients, 152; Child–Pugh class A:B, 150:33; Barcelona Clinic Liver Cancer stage B:C, 59:124; initial line use, 64). Clinical factors with prognostic influence on PFS in these patients were retrospectively evaluated.ResultsThe median observation period was 7.2 months (interquartile range, 3.2–10.4). History of atezolizumab plus bevacizumab (Atz/Bev) treatment was the only significant prognostic factor for PFS at introduction of Dur/Tre in multivariate analysis (hazard ratio 2.040, p = 0.028) (median PFS: without vs. with = 5.6 vs. 2.7 months, p < 0.001). Although immune‐mediated adverse events (imAE) occurrence was only significant in univariate analysis, when objective response and disease control rates were examined according to imAE positivity (any grade) at the time of analysis, those were noted in 14.4% and 39.2%, respectively, of patients without imAE, while in patients with imAE (any grade), they were noted in 18.2% and 56.1%, respectively (p = 0.523 and p = 0.038, respectively).ConclusionHistory of Atz/Bev treatment may be an independent clinical factor for poor PFS at Dur/Tre introduction.