bluuebunny/crossref_metadata_2025_split huggingface dataset:
Altered EEG resting-state large-scale brain network dynamics in euthymic bipolar disorder patients
Authors: Damborská Alena , Piguet Camille , Aubry Jean-Michel , Dayer Alexandre G. , Michel Christoph M. , Berchio Cristina 
Published: June 2019

AbstractBackgroundNeuroimaging studies provided evidence for disrupted resting-state functional brain network activity in bipolar disorder (BD). Electroencephalographic (EEG) studies found altered temporal characteristics of functional EEG microstates during depressive episode within different affective disorders. Here we investigated whether euthymic patients with BD show deviant resting-state large-scale brain network dynamics as reflected by altered temporal characteristics of EEG microstates.MethodsWe used high-density EEG to explore between-group differences in duration, coverage and occurrence of the resting-state functional EEG microstates in 17 euthymic adults with BD in on-medication state and 17 age- and gender-matched healthy controls. Two types of anxiety, state and trait, were assessed separately with scores ranging from 20 to 80.ResultsMicrostate analysis revealed five microstates (A-E) in global clustering across all subjects. In patients compared to controls, we found increased occurrence and coverage of microstate A that did not significantly correlate with anxiety scores.ConclusionOur results provide neurophysiological evidence for altered large-scale brain network dynamics in BD patients and suggest the increased presence of A microstate to be an electrophysiological trait characteristic of BD.
Evolutionary time best explains the latitudinal diversity gradient of living freshwater fish diversity
Authors: Miller Elizabeth Christina , Román-Palacios Cristian 
Published: June 2019

AbstractAimThe evolutionary causes of the latitudinal diversity gradient are debated. Hypotheses have ultimately invoked either faster rates of diversification in the tropics, or more time for diversification due to the tropical origins of higher taxa. Here we perform the first test of the diversification rate and time hypotheses in freshwater ray-finned fishes, a group comprising nearly a quarter of all living vertebrates.LocationGlobal.Time period368–0 mya.Major taxa studiedExtant freshwater ray-finned fishes.MethodsUsing a mega-phylogeny of actinopterygian fishes and a global database of occurrence records, we estimated net diversification rates, the number of colonizations and regional colonization times of co-occurring species in freshwater drainage basins. We used Generalized Additive Models to test whether these factors were related to latitude. We then compared the influence of diversification rates, colonization numbers, colonization times and surface area on species richness, and how these factors are related to each other.ResultsWhile both diversification rates and time were related to richness, time had greater explanatory power and was more strongly related to latitude than diversification rates. Other factors (basin surface area, number of colonizations) also helped explain richness but were unrelated to latitude. The world’s most diverse freshwater basins (Amazon, Congo rivers) were dominated by lineages with Mesozoic origins. The temperate groups dominant today arrived near the K-Pg boundary, leaving comparatively less time to build richness. Diversification rates and colonization times were inversely related: recently colonized basins had the fastest rates, while ancient species-rich faunas had slower rates.Main conclusionsWe concluded that time is the lead driver of latitudinal richness disparities in freshwater fish faunas. We suggest that the most likely path to building very high species richness is through diversification over long periods of time, rather than diversifying quickly.
Low-Density Granulocytes are a novel immunopathological feature in both Multiple Sclerosis and Neuromyelitis optica spectrum disorder
Authors: Ostendorf Lennard , Mothes Ronja , van Koppen Sofie , Lindquist Randall L. , Bellmann-Strobl Judith , Asseyer Susanna , Ruprecht Klemens , Alexander Tobias , Niesner Raluca A. , Hauser Anja E. , Paul Friedemann , Radbruch Helena 
Published: June 2019

AbstractObjectiveTo investigate whether low-density granulocytes (LDGs) are a immunophenotypic feature of patients with multiple sclerosis (MS) and/or neuromyelitis optica spectrum disorder (NMOSD).MethodsBlood samples were collected from 26 patients with NMOSD and 20 patients with MS, as well as from 18 patients with Systemic Lupus Erythematosus (SLE) and 23 Healthy Donors (HD). We isolated peripheral blood mononuclear cells (PBMCs) with density gradient separation and stained the cells with antibodies against CD14, CD15, CD16, and CD45, and analysed the cells by flow cytometry or imaging flow cytometry. We defined LDGs as CD14-CD15high and calculated their share in total PBMC leukocytes (CD45+) as well as the share of CD16hi LDGs. Clinical data on disease course, medication, and antibody status were obtained.ResultsLDGs were significantly more common in MS and NMOSD than in HDs, comparable to SLE samples (median values HD 0.2%, MS 0.9%, NMOSD 2.1%, SLE 4.3%). 0/23 of the HDs, but 17/20 NMOSD and 11/17 MS samples as well as 13/15 SLE samples had at least 0.7 % LDGs. NMOSD patients without continuous immunosuppressive treatment had significantly more LDGs compared to their treated counterparts. LDG nuclear morphology ranged from segmented to rounded, suggesting a heterogeneity within the group.ConclusionLDGs are a feature of the immunophenotype in some patients with MS and NMOSD.
Microstimulation of primate neocortex targeting striosomes induces negative decision-making
Authors: Amemori Satoko , Amemori Ken-ichi , Yoshida Tomoko , Papageorgiou Georgios K. , Xu Rui , Shimazu Hideki , Desimone Robert , Graybiel Ann M. 
Published: June 2019

AbstractAffective judgment and decision-making are strongly modulated by the pregenual anterior cingulate (pACC) and caudal orbitofrontal (cOFC) cortical regions. By combining MRI-guided electrical microstimulation with viral tracing methods in non-human primates, we demonstrate that circumscribed pACC and cOFC microstimulation sites that induce negative decision-making preferentially project to striosomes in the anterior striatum. These results outline a behaviorally important circuit from pACC/cOFC to striosomes causally modulating decision-making under emotional conflict.
Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation
Authors: King Matthew R. , Petry Sabine 
Published: June 2019

AbstractPhase separation of substrates and effectors is proposed to enhance biological reaction rates and efficiency. TPX2 is an effector of microtubule nucleation in spindles, and functions with the substrate tubulin by an unknown mechanism. Here, we show that TPX2 phase separates into a co-condensate with tubulin, which mediates microtubule nucleation in vitro and in isolated cytosol. TPX2-tubulin co-condensation preferentially occurs on pre-existing microtubules at the endogenous and physiologically relevant concentration of TPX2. Truncation and chimera versions of TPX2 directly demonstrate that TPX2-tubulin co-condensation enhances the efficiency of TPX2-mediated microtubule nucleation. Finally, the known inhibitor of TPX2, the importin-α/β heterodimer, regulates both co-condensation and activity. Our study demonstrates how regulated phase separation can simultaneously enhance reaction efficiency and spatially coordinate microtubule nucleation, which may facilitate rapid and accurate spindle formation.
Prepontine non-giant neurons drive flexible escape behavior in zebrafish
Authors: Marquart Gregory D. , Tabor Kathryn M. , Bergeron Sadie A. , Briggman Kevin L. , Burgess Harold A. 
Published: June 2019

AbstractMany species execute ballistic escape reactions to avoid imminent danger. Despite fast reaction times, responses are often highly regulated, reflecting a trade-off between costly motor actions and perceived threat level. However, how sensory cues are integrated within premotor escape circuits remains poorly understood. Here we show that in zebrafish, less precipitous threats elicit a delayed escape, characterized by flexible trajectories, that are driven by a cluster of 38 prepontine neurons that are completely separate from the fast escape pathway. Whereas neurons that initiate rapid escapes receive direct auditory input and drive motor neurons, input and output pathways for delayed escapes are indirect, facilitating integration of cross-modal sensory information. Rapid decision making in the escape system is thus enabled by parallel pathways for ballistic responses and flexible delayed actions.
Compound heterozygosity of the gamma-glutamyl carboxylase mutants V255M and S300F causes pseudoxanthoma elasticum-like disease through impaired processivity
Authors: Rishavy Mark A. , Hallgren Kevin W. , Runge Kurt W. , Berkner Kathleen L. 
Published: June 2019

ABSTRACTVitamin K-dependent (VKD) protein activities require carboxylated Glus (Glas) generated by the gamma-glutamyl carboxylase. Some carboxylase mutations cause severe bleeding, while others cause pseudoxanthoma elasticum (PXE)-like associated with excessive calcification. How carboxylase mutations cause PXE-like was unknown. We analyzed two mutants (V255M and S300F) whose compound heterozygosity causes PXE-like. Substrates derived from VKD proteins important to calcification (MGP) or clotting (factor IX) were studied, which contained the Gla domain and exosite-binding domain that mediates carboxylase binding. Surprisingly, the V255M mutant was more active (4-5 fold) than wild type carboxylase, while S300F activity was low. The V255M results suggested faster substrate release, which could impact carboxylase processivity, where the carboxylase remains bound to VKD proteins throughout multiple Glu to Gla conversions. To assess mutant processivity, we performed a novel challenge assay in which MGP-carboxylase and factor IX-carboxylase complexes were reacted in the presence of excess challenge VKD peptide. Tight complexes between VKD proteins and wild type carboxylase excluded access of the challenge peptide during the carboxylation of VKD protein in the complex. In contrast, VKD protein complexes with V255M or S300F allowed promiscuous access of challenge peptide. Both mutants therefore impair processivity. Most of the V255M product was carboxylated challenge peptide, which could explain mild PXE-like observed in the proband’s mother and aunt. Both have wild type and V255M carboxylase alleles; however, higher V255M production of a potentially defective MGP product could account for their phenotype. The results are an important advance in understanding why carboxylase mutations cause the PXE-like disease.
Effect of habitat quality, microclimatic conditions and waste water contamination on diversity & distribution of Collembola community
Authors: Abbas Mohammad Jalaluddin , Parwez Hina 
Published: June 2019

AbstractSoil biodiversity is undergoing dramatic changes due to human and climatic interference, habitat loss and fragmentation due to land use change, increasing pollution and degradation in terrestrial ecosystems. The overall effect of habitat loss is on the loss of soil biodiversity which might be due to negative response of substrate quality. Adverse microclimatic conditions may also be detrimental to species specific communities along with waste water contamination. Thus, an investigation has been carried out to ascertain the effect of habitat quality, microclimatic conditions and waste water contamination on diversity and distribution of Collembola community at Aligarh. For this, 4 samples have been taken monthly for a period of one year from two different study sites of Aligarh along with edapho-chemical properties. We observed that, density of Collembola (18.8 ind./sample) was always approximately three times more in agriculture site than in wasteland (7.6 ind./sample) site. Population of Collembola was statistically positive significant with reference to soil organic matter % (r = 0.621, P>0.05), SOC % (r = 0.622, P>0.05), Available nitrogen (r = 0.622, P>0.05) and Potash (r = 0.562, P>0.05) in Agriculture site whereas these all were statistically insignificant in Wasteland site. This observation suggests that quality of habitat has a direct effect on the diversity and population of Collembolans. The wasteland site which is a sewage filled from the nearby colonies also it is a dumping ground of waste building material under Municipal Corporation. Hence, the soil is highly contaminated as compared to the agricultural soil.
Cdc48/VCP and endocytosis regulate TDP-43 and FUS toxicity and turnover
Authors: Liu Guangbo , Byrd Aaron , Pei Fen , Buchanan Allison , Basha Eman , Warner Amanda , Buchan J. Ross 
Published: June 2019

AbstractAmyotrophic lateral sclerosis (ALS) is a fatal motor neuron degenerative disease. TDP-43 (TAR DNA-binding protein 43) and FUS (fused in sarcoma) are aggregation-prone RNA-binding proteins that in ALS can mis-localize to the cytoplasm of affected motor neuron cells, often forming cytoplasmic aggregates in the process. Such mis-localization and aggregation are implicated in ALS pathology, though the mechanisms of TDP-43 and FUS cytoplasmic toxicity remains unclear. Recently, we determined that the endocytic function aids turnover of TDP-43 and reduces TDP-43 toxicity. Here, we identified that Cdc48 and Ubx3, a Cdc48 co-factor implicated in endocytic function, regulates the turnover and toxicity of TDP-43 and FUS expressed in S. cerevisiae. Cdc48 physically interacts and co-localizes with TDP-43, as does VCP in ALS patient tissue. In yeast, FUS toxicity also depends strongly on endocytic function, but not autophagy under normal conditions. FUS expression also impairs endocytic function, as previously observed with TDP-43. Taken together, our data identifies a role for Cdc48/VCP and endocytosis function in regulating TDP-43 and FUS toxicity and turnover. Furthermore, endocytic dysfunction may be a common defect affecting cytoplasmic clearance of ALS aggregation-prone proteins and may represent a novel therapeutic target of promise.
Bioactive compounds from Chrysosporium multifidum, a fungus isolated from Hermetia illucens gut microbiota
Authors: Correa Yesenia , Cabanillas Billy , Jullian Valérie , Álvarez Daniela , Castillo Denis , Dufloer Cédric , Bustamante Beatriz , Roncal Elisa , Neyra Edgar , Sheen Patricia , Sauvain Michel 
Published: June 2019

AbstractThe gut microbiota of insects contains a wide range of organisms that protect them against the attack of pathogens by releasing various types of bioactive compounds. In the present study, we report the isolation and identification of the fungus Chrysosporium multifidum as a component of the microbiota from the larval gut of Hermetia illucens. Extract from the broth culture of C. multifidum showed moderate activity on a strain of methicillin-resistant Staphylococcus aureus (MRSA). The bioguided isolation of the extract resulted in the characterization of six α-pyrone derivatives (1-6) and one diketopiperazines (7), among them 5,6-dihydro-4-methoxy-6-(1-oxopentyl)-2H-pyran-2-one (4) showed the best activity (IC50 = 11.4 ± 0.7 µg/ml and MIC = 62.5 μg/ml).
Spatial distribution of epibionts on olive ridley sea turtles at Playa Ostional, Costa Rica
Authors: Robinson Nathan J. , Lazo-Wasem Emily , Butler Brett O. , Lazo-Wasem Eric A. , Zardus John D. , Pinou Theodora 
Published: June 2019

ABSTRACTThere is a wealth of published information on the epibiont communities of sea turtles, yet many of these studies have exclusively sampled epibionts found only on the carapace. Considering that epibionts may be found on almost all body-surfaces and that it is highly plausible to expect different regions of the body to host distinct epibiont taxa, there is a need for quantitative comparative studies to investigate spatial variation in the epibiont communities of turtles. To achieve this, we measured how total epibiont abundance and biomass on olive ridley turtlesLepidochelys olivaceavaries among four body-areas of the hosts (n = 30). We show that epibiont loads on olive ridleys are higher, both in terms of number and biomass, on the skin than they are on the carapace or plastron. This contrasts with previous findings for other hard-shelled sea turtles, where epibionts are usually more abundant on the carapace. Moreover, the arguably most ubiquitous epibiont taxon for other hard-shelled sea turtles, the barnacleChelonibia spp., only occurs in relatively low numbers on olive ridleys, while the barnaclesStomatolepas elegansandPlatylepas hexastylosare far more abundant. We postulate that these differences between the epibiont communities of different sea turtle taxa could indicate that the carapaces of olive ridley turtles provide a more challenging substratum for epibionts than do the hard shells of other sea turtles. In addition, we conclude that it is important to conduct full body surveys when attempting to produce a holistic qualitative or quantitative characterization of the epibiont communities of sea turtles.
Socio-demographic correlates and clustering of non-communicable diseases risk factors among reproductive aged women of Nepal: Results from Nepal Demographic Health Survey 2016
Authors: Bista Bihungum , Dhungana Raja Ram , Chalise Binaya , Pandey Achyut Raj 
Published: June 2019

AbstractGlobally, Non Communicable Diseases (NCDs) are the major killer diseases, majority of which are attributable to common risk factors like smoking, alcohol intake, physical inactivity and low fruits/vegetable consumption. Clustering of these risk factors increases the risk of developing NCDs. The occurrence of NCDs among women is alarmingly high, and this invites impact on upcoming generation too. So, this study aimed to assess the prevalence and clustering of selected risk factors and their socio-demographic determinants in Nepalese women using Nepal Demographic and Health Survey (NDHS) 2016 data.NDHS applied stratified multi-stage cluster sampling to reach to the individual respondent for representing the whole nation. This study included analysis of data of 6,396 women of age 15 to 49 years. Chi-square test for bivariate analysis and multiple poisson regression to calculate adjusted prevalence ratio was applied.A total of 8.91% participants were current smoker. Similarly, 22.19% and 11.45% of participants were overweight and hypertensive respectively. Around 6.02% of participants had a co-occurrence of two NCDs risk factors. Smoking, overweight and hypertension were significantly associated with age, education, province, wealth index and ethnicity. Risk factors were more likely to cluster in women aged 40-49 years (APR=2.95, CI: 2.58-3.38), widow/separated (APR=3.09, CI: 2.24-4.28) and Dalit) (APR=1.34, CI: 1.17-1.55).This study found that NCD risk factors were disproportionately distributed by age, education, socio-economic status and ethnicity and clustered in more vulnerable groups such as widow/separated, Dalit and Janajati.
Diversity and Life-cycle Analysis of Pacific Ocean Zooplankton by Videomicroscopy and DNA Barcoding. I. Cnidaria
Authors: Bryant Peter J. , Arehart Timothy E. 
Published: June 2019

AbstractMost, but not all cnidarian species in the classes Hydrozoa, Scyphozoa and Anthozoa have a life cycle in which a colonial, asexually reproducing hydroid phase alternates with a free-swimming, sexually reproducing medusa phase that, in the hydrozoans, is usually microscopic. Hydrozoan medusae were collected by zooplankton tows in Newport Bay and the Pacific Ocean near Newport Beach, California, and hydroid colonies were collected from solid substrates in the same areas. Specimens were documented by videomicroscopy, preserved in ethanol, and sent to the Canadian Centre for DNA Barcoding at the University of Guelph, Ontario, Canada for DNA barcoding.Among the order Anthomedusae (athecate hydroids), DNA barcoding allowed for the discrimination between the medusae of eight putative species of Bougainvillia, and the hydroid stages were documented for two of these. The medusae of three putative species of Amphinema were identified, and the hydroid stages were identified for two of them. DNA barcodes were obtained from medusae of one species of Cladonema, one adult of the By-the wind Sailor, Velella Velella, five putative species of Corymorpha with the matching hydroid phase for one; and Coryne eximia, Turritopsis dohrnii and Turritopsis nutricula with the corresponding hydroid phases. The actinula larvae and hydroid for the pink-hearted hydroid Ectopleura crocea were identified and linked by DNA barcoding.Among the order Leptomedusae (thecate hydroids) medusae were identified for Clytia elsaeoswaldae, Clytia gracilis and Clytia sp. 701 AC and matched with the hydroid phases for the latter two species. Medusae were matched with the hydroid phases for two species of Obelia (including O. dichotoma) and Eucheilota bakeri. Obelia geniculata was collected as a single hydroid. DNA barcodes were obtained for hydroids of Orthopyxis everta and three other species of Orthopyxis.The medusa of one member of the family Solmarisidae, representing the order Narcomedusae, and one member (Liriope tetraphylla) of the order Trachymedusae were recognized as medusae.In the Scyphozoa, DNA barcoding confirmed the planktonic larval stage (ephyra) of the Moon Jelly, Aurelia aurita, the adult medusa of which is occasionally common in and around Newport Bay. In the Anthozoa, antipathula larvae were identified from the Onion Anemone, Paranthus rapiformis and a cerinula larva was identified from the Tube-dwelling Anemone, Isarachnanthus nocturnus. We have yet to find the adults of these species locally.
A 3D assembly model retrieval method based on assembly information
Authors: Qiao Hu , Wu Qingyun , Yu Songlin , Du Jiang , Xiang Ying 
Published: June 2019

Purpose
The purpose of this paper is to propose a three-dimensional (3D) assembly model retrieval method based on assembling semantic information to address semantic mismatches, poor accuracy and low efficiency in existing 3D assembly model retrieval methods.

Design/methodology/approach
The paper proposes an assembly model retrieval method. First, assembly information retrieval is performed, and 3D models that conform to the design intention of the assembly are found by retrieving the code. On this basis, because there are conjugate subgraphs between attributed adjacency graphs (AAG) that have an assembly relationship, the assembly model geometric retrieval is translated into a problem of finding AAGs with a conjugate subgraph. Finally, the frequent subgraph mining method is used to retrieve AAGs with conjugate subgraphs.

Findings
The method improved the efficiency and accuracy of assembly model retrieval.

Practical implications
The examples illustrate the specific retrieval process and verify the feasibility and reasonability of the assembly model retrieval method in practical applications.

Originality/value
The assembly model retrieval method in the paper is an original method. Compared with other methods, good results were obtained.