bluuebunny/crossref_metadata_2025_split huggingface dataset:
Few‐layer graphene coated on indium tin oxide electrodes prepared by chemical vapor deposition and their enhanced glucose electrooxidation activity
Authors: Caglar Aykut , Ulas Berdan , Sahin Ozlem , Demir Kivrak Hilal 
Published: June 2019

ABSTRACTAt present, few‐layer graphene is deposited on copper (Cu) foil by chemical vapor deposition (CVD) method. Then, the few‐layer graphenes produced on the Cu foil are coated onto the indium tin oxide (ITO) electrode to investigate their glucose electrooxidation activities. Hexane and hydrogen flow rate and deposition time parameters with CVD method are examined on different Cu foils. These electrodes are characterized by scanning electron microscopy‐energy dispersive X‐ray analysis (SEM‐EDX), X‐ray photoelectron spectroscopy (XPS), and Raman spectroscopy. Furthermore, glucose electrooxidation is examined with cyclic voltammetry (CV), chronoamperometry (CA), and electrochemical impedance spectroscopy (EIS) measurements. One could note that the graphene network is clearly visible from SEM images. The deconvoluted XPS spectrum indicates that carbon appeared in the form of non‐oxygenated ring C atoms for few‐layer graphene. The few‐layer graphene structure is confirmed by Raman analysis. Few‐layer graphene/ITO produced at 5 sccm Hexane and 50 sccm hydrogen flow rate and 20 minutes deposition time (G7/ITO) reveals the best electrode activity. The specific activity of G7/ITO electrode is obtained as 6.58 mA cm−2. According to CV, CA, and EIS results, G7/ITO electrode has high electrocatalytic activity, stability, and resistance in comparison with other electrodes.
Electrochemical Impedance Spectroscopy of Electrospun La0.6Sr0.4Co0.2Fe0.8O3–δ Nanorod Cathodes for Intermediate Temperature – Solid Oxide Fuel Cells
Authors: Costamagna P. , Sanna C. , Campodonico A. , Sala E. M. , Sažinas R. , Holtappels P. 
Published: June 2019

AbstractElectrospun La0.6Sr0.4Co0.2Fe0.8O3–δ (LSCF) nanorod cathodes are investigated. Morphological characterization shows that the nanorods are arranged into a 3‐D stochastic structure, with multiple contact points with each other. Experimental characterization of symmetrical button cells through electrochemical impedance spectroscopy demonstrates an electrode polarization resistance Rp = 3.9 Ω cm2 at 940 K and Rp = 10.9 Ω cm2 at 890 K. The impedance results, measured from 890 K up to 1,200 K, show that the depressed Gerischer behavior is not an intrinsic feature of the electrode, since it appears only at certain well defined temperatures, and then it tends to disappear for other operating temperatures. Equivalent circuit model fitting is accomplished through the depressed Gerischer element, and also through an alternative circuit based on a normal Gerischer coupled to an RQ element. The superior fitting capability of the latter equivalent circuit is demonstrated. The latter circuit allows to assess separately the impedance contribution of the electrode bulk, associated to the Gerischer element, and of the electrode/electrolyte interface, associated to the RQ element. Both the related polarization resistances are larger with the electrospun LSCF 3‐D random nanorod electrode, rather than with an electrospun LSCF electrode with unbroken nanofibers laying neatly in‐plane over the electrolyte.
An integrative Bayesian approach to matrix‐based analysis in neuroimaging
Authors: Chen Gang , Bürkner Paul‐Christian , Taylor Paul A. , Li Zhihao , Yin Lijun , Glen Daniel R. , Kinnison Joshua , Cox Robert W. , Pessoa Luiz 
Published: June 2019

AbstractUnderstanding the correlation structure associated with brain regions is a central goal in neuroscience, as it informs about interregional relationships and network organization. Correlation structure can be conveniently captured in a matrix that indicates the relationships among brain regions, which could involve electroencephalogram sensors, electrophysiology recordings, calcium imaging data, or functional magnetic resonance imaging (FMRI) data—We call this type of analysis matrix‐based analysis, or MBA. Although different methods have been developed to summarize such matrices across subjects, including univariate general linear models (GLMs), the available modeling strategies tend to disregard the interrelationships among the regions, leading to “inefficient” statistical inference. Here, we develop a Bayesian multilevel (BML) modeling framework that simultaneously integrates the analyses of all regions, region pairs (RPs), and subjects. In this approach, the intricate relationships across regions as well as across RPs are quantitatively characterized. The adoption of the Bayesian framework allows us to achieve three goals: (a) dissolve the multiple testing issue typically associated with seeking evidence for the effect of each RP under the conventional univariate GLM; (b) make inferences on effects that would be treated as “random” under the conventional linear mixed‐effects framework; and (c) estimate the effect of each brain region in a manner that indexes their relative “importance”. We demonstrate the BML methodology with an FMRI dataset involving a cognitive‐emotional task and compare it to the conventional GLM approach in terms of model efficiency, performance, and inferences. The associated program MBA is available as part of the AFNI suite for general use.
Cutaneous lymphomas—An update 2019
Authors: Kempf Werner , Zimmermann Anne‐Katrin , Mitteldorf Christina 
Published: June 2019

AbstractPrimary cutaneous lymphomas (CL) are the second most common form of extranodal lymphomas. Cutaneous T‐cell lymphomas represent the majority. They are classified according to the WHO classification 2017 and the updated WHO‐EORTC 2018 published in the fourth edition of the WHO classification for Skin Tumors monograph. Primary cutaneous acral CD8+ T‐cell lymphoma and EBV‐positive mucocutaneous ulcer have been listed as new provisional entities. Moreover, the histological and genetic spectrum of lymphomatoid papulosis has been expanded. Recently, prognostic subtypes were delineated for some entities and subtypes of CL such as folliculotropic mycosis fungoides and marginal zone lymphoma. Since CL show overlapping histological features, clinico‐pathological correlation is of outmost importance for the diagnosis. Recent studies revealed new biomarkers and genetic alterations underlying the pathogenesis of CL. Moreover, targeted therapies have widened the treatment options particularly for aggressive lymphomas.
Rare mature B‐cell lymphomas in children and adolescents
Authors: Woessmann Wilhelm , Quintanilla‐Martinez Leticia 
Published: June 2019

AbstractPediatric‐type follicular lymphoma (PTFL), pediatric nodal marginal zone lymphoma (pnMZL), and large B‐cell lymphoma (LBCL) with IRF4 rearrangement have been introduced into the current World Health Organization (WHO) classification. They account for 5% to 10% of mature B‐cell lymphomas in children and adolescents. Both PTFL and pnMZL predominantly affect male adolescents and usually present with localized lymphadenopathy in the head and neck region. The cells within the follicles of PTFL typically show high‐grade cytology, IGH monoclonality and lack the t(14;18) chromosomal alteration. In contrast, pnMZL is characterized by progressive transformation of germinal center (PTGC)‐like features and interfollicular proliferation of the cells with expansion of the marginal zones with diffuse areas. Watch and wait after complete resection seems an adequate therapy with chemotherapy restricted to incompletely resected disease. All children with PTFL and pnMZL reported, so far, survived. B‐cell lymphomas presenting in the Waldeyer's ring are characterized by the expression of IRF4/MUM1 and often associated with IRF4 rearrangements. Because of the frequent diffuse component, treatment often follows current protocols for mature B‐NHL. The prognosis is excellent.
The modern approach to mantle cell lymphoma
Authors: Rule Simon 
Published: June 2019

AbstractMantle Cell Lymphoma is a rare and generally aggressive form of non Hodgkin lymphoma. Our understanding of the pathophysiology of this disease is improving and whilst risk factors are understood, treatments are not yet tailored towards these. The treatment algorithm in the front line is well established for older and younger patients and observation is the norm for a subset of patients although these are not well characterised as yet. In the relapse setting the role of novel agents, especially the BTK inhibitors is becoming established and combination approaches look promising. Trials are beginning to challenge the role of chemotherapy against the novel agents especially as part of front line therapy. As a consequence it is likely we will see a paradigm shift in the management of this disease in the next few years.
LncRNA LOXL1‐AS1/miR‐let‐7a‐5p/EGFR‐related pathway regulates the doxorubicin resistance of prostate cancer DU‐145 cells
Authors: Bai Tianliang , Liu Yabin , Li Binghui 
Published: June 2019

AbstractOur study aimed to investigate the effects of lncRNA LOXL1‐AS1/miR‐let‐7a‐5p/EGFR‐axis on prostate cancer (PCa) progression. Microarray analysis was conducted to determine differentially expressed lncRNAs and mRNAs. Gene Set Enrichment analysis was implemented for verification of dys‐regulated signaling pathways between DU‐145 cells and doxorubicin‐resistant prostate cancer DU‐145 cells. Relative expression of lncRNA LOXL1‐AS1 in doxorubicin‐resistant prostate cancer DU‐145 cells was analyzed by qRT‐PCR. CCK‐8 assay and flow cytometry analysis were employed to detect cell proliferation and apoptosis, respectively. Cell migration was performed by transwell assay. Furthermore, targeted relationships between lncRNA LOXL1‐AS1 and miR‐let‐7a‐5p, as well as miR‐let‐7a‐5p and EGFR were predicted using bioinformatics analysis and validated by dual‐luciferase reporter gene assay. Besides, tumor xenograft assay was utilized for verification of the roles of LOXL1‐AS1 in PCa progression in vivo. Microarray analysis showed that lncRNA LOXL1‐AS1 and EGFR were both downregulated, while miR‐let‐7a‐5p was upregulated in doxorubicin‐resistant prostate cancer DU‐145 cells. MiR‐let‐7a‐5p could target both lncRNA LOXL1‐AS1 and EGFR to affect PCa progression. Upregulation of lncRNA LOXL1‐AS1 promoted cell proliferation and migration, while suppressed cell apoptosis. Besides, it was further confirmed that EGFR was downregulated in drug‐resistant PCa cells and negatively correlated with miR‐let‐7a‐5p. Tumor xenograft assay verified that silence of lncRNA LOXL1‐AS1 inhibited the tumor growth in vivo in DU‐145 cells. Our results demonstrated that the lncRNALOXL1‐AS1/miR‐let‐7a‐5p/EGFR axis significantly affected proliferation, migration, and apoptosis of drug‐resistant DU‐145 Cells, which may provide us with a potential treatment strategy for drug‐resistant PCa patients. © 2019 IUBMB Life, 2019
A Pilot Study Showing Acute Inhibitory Effect of GLP‐1 on the Bone Resorption Marker CTX in Humans
Authors: Nissen Anne , Marstrand Simone , Skov‐Jeppesen Kirsa , Bremholm Lasse , Hornum Mads , Andersen Ulrik B , Holst Jens Juul , Rosenkilde Mette Marie , Hartmann Bolette 
Published: June 2019

ABSTRACTBones have been suggested to be a target for glucagon‐like peptide ‐1 (GLP‐1); however, studies of the effects on human bones so far have given diverging results. We hypothesized that GLP‐1, together with glucagon‐like peptide‐2 and glucose‐dependent insulinotropic polypeptide, plays a role in the gut–bone axis. We examined the acute effect of three GLP‐1 receptor ligands [GLP‐1 (7‐36)amide, GLP‐1 (9‐36)amide, and exenatide] on markers of bone remodeling. Eight healthy, normal‐weight participants, with a mean age of 24.3 years, were studied for 4 days in a double‐blinded, randomized clinical trial. Blood was collected before and after s.c. injection of GLP‐1 (7‐36)amide (1.5 nmol/kg), GLP‐1 (9‐36)amide (1.5 nmol/kg), exenatide (2.4 nmol/subject), or saline. Plasma was analyzed for bone markers and for osteoprotegerin (OPG), PTH, and IGF‐1 levels. All ligands were tested in vitro for their cAMP‐inducing activity on the human GLP‐1 receptor. GLP‐1 (7‐36)amide decreased CTX‐levels, compared with placebo (area under the curve [AUC] ±SD 0 to 120 min = –2143 ± 1294 % × min versus –883 ± 1557 % × min; p < 0.05). No difference was observed between placebo and GLP‐1 (9‐36)amide, or between placebo and exenatide, although exenatide had a similar potency as GLP‐1 (7‐36)amide for cAMP formation in vitro (EC50 of 0.093 and 0.054 nmol/L). However, exenatide reached maximum plasma concentration at 90 min versus 15 min for GLP‐1 (7‐36)amide, and plasma CTX was significantly decreased during the second hour of the study after exenatide injections compared with placebo (AUC ±SD –463.1 ± 218 % × min and –136 ± 91 % × min; p < 0.05). There was no effect of the injections on bone formation markers (P1NP and osteocalcin) or on OPG, PTH and IGF‐1 levels. In conclusion, we show that GLP‐1 receptor agonists, but not the primary metabolite GLP‐1 (9‐36)amide, decrease bone resorption, and suggest that GLP‐1 may be part of the gut–bone axis. © 2019 The Authors. JBMR Plus is published by Wiley Periodicals, Inc. on behalf of the American Society for Bone and Mineral Research.
Chondrocyte-Specific RUNX2 Overexpression Accelerates Post-traumatic Osteoarthritis Progression in Adult Mice
Authors: Catheline Sarah E , Hoak Donna , Chang Martin , Ketz John P , Hilton Matthew J , Zuscik Michael J , Jonason Jennifer H 
Published: June 2019

ABSTRACT
RUNX2 is a transcription factor critical for chondrocyte maturation and normal endochondral bone formation. It promotes the expression of factors catabolic to the cartilage extracellular matrix and is upregulated in human osteoarthritic cartilage and in murine articular cartilage following joint injury. To date, in vivo studies of RUNX2 overexpression in cartilage have been limited to forced expression in osteochondroprogenitor cells preventing investigation into the effects of chondrocyte-specific RUNX2 overexpression in postnatal articular cartilage. Here, we used the Rosa26Runx2 allele in combination with the inducible Col2a1CreERT2 transgene or the inducible AcanCreERT2 knock-in allele to achieve chondrocyte-specific RUNX2 overexpression (OE) during embryonic development or in the articular cartilage of adult mice, respectively. RUNX2 OE was induced at embryonic day 13.5 (E13.5) for all developmental studies. Histology and in situ hybridization analyses suggest an early onset of chondrocyte hypertrophy and accelerated terminal maturation in the limbs of the RUNX2 OE embryos compared to control embryos. For all postnatal studies, RUNX2 OE was induced at 2 months of age. Surprisingly, no histopathological signs of cartilage degeneration were observed even 6 months following induction of RUNX2 OE. Using the meniscal/ligamentous injury (MLI), a surgical model of knee joint destabilization and meniscal injury, however, we found that RUNX2 OE accelerates the progression of cartilage degeneration following joint trauma. One month following MLI, the numbers of MMP13-positive and TUNEL-positive chondrocytes were significantly greater in the articular cartilage of the RUNX2 OE joints compared to control joints and 2 months following MLI, histomorphometry and Osteoarthritis Research Society International (OARSI) scoring revealed decreased cartilage area in the RUNX2 OE joints. Collectively, these results suggest that although RUNX2 overexpression alone may not be sufficient to initiate the OA degenerative process, it may predetermine the rate of OA onset and/or progression following traumatic joint injury. © 2019 American Society for Bone and Mineral Research.
LINC01579 promotes cell proliferation by acting as a ceRNA of miR‐139‐5p to upregulate EIF4G2 expression in glioblastoma
Authors: Chai Yang , Xie Mingxiang 
Published: June 2019

AbstractGlioblastoma (GBM), a malignant and lethal tumor, remains a big threat to human health and life. Increasing explorations have confirmed that long noncoding RNAs are involved in the tumorigenesis and development of multiple cancers. Nevertheless, the regulatory mechanism of (long intergenic nonprotein coding RNA 1579 LINC01579) in GBM remains to be investigated. In this study, the expression of LINC01579 was upregulated in GBM cells and LINC01579 knockdown inhibited cell proliferation as well as promoted cell apoptosis. Additionally, LINC01579 acted as a sponge for miR‐139‐5p in GBM and eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) was found to be a downstream target of miR‐139‐5p. Furthermore, the positive correlation of LINC01579 and EIF4G2 as well as the converse correlation between miR‐139‐5p and LINC01579 (or EIF4G2) were revealed by the experiments. Based on rescue assays, EIF4G2 overexpression or miR‐139‐5p inhibitor partially recovered the function of LINC01579 knockdown on cell proliferation and apoptosis. In summary, the results of this study verified that LINC01579 modulated cell proliferation and cell apoptosis in GBM by competitively binding with miR‐139‐5p to regulate EIF4G2, which provided a new clue to figure out potential therapy for patients suffered from GBM.
Slc25a17 acts as a peroxisomal coenzyme A transporter and regulates multiorgan development in zebrafish
Authors: Kim Yong‐Il , Nam In‐Koo , Lee Dong‐Kyu , Bhandari Sushil , Charton Lennart , Kwak SeongAe , Lim Jae‐Young , Hong KwangHeum , Kim Se‐Jin , Lee Joon No , Kwon Sung Won , So Hong‐Seob , Linka Nicole , Park Raekil , Choe Seong‐Kyu 
Published: June 2019

AbstractSlc25a17 is known as a peroxisomal solute carrier, but the in vivo role of the protein has not been demonstrated. We found that the zebrafish genome contains twoslc25a17genes that function redundantly, but additively. Notably, peroxisome function inslc25a17knockdown embryos is severely compromised, resulting in an altered lipid composition. Along the defects found in peroxisome‐associated phenotypic presentations, we highlighted that development of the swim bladder is also highly dependent on Slc25a17 function. As Slc25a17 showed substrate specificity towards coenzyme A (CoA), injecting CoA, but not NAD+, rescued the defective swim bladder induced byslc25a17knockdown. These results indicated that Slc25a17 acts as a CoA transporter, involved in the maintenance of functional peroxisomes that are essential for the development of multiple organs during zebrafish embryogenesis. Given high homology in protein sequences, the role of zebrafish Slc25a17 may also be applicable to the mammalian system.
An antibody to Notch3 reverses the skeletal phenotype of lateral meningocele syndrome in male mice
Authors: Yu Jungeun , Siebel Christian W. , Schilling Lauren , Canalis Ernesto 
Published: June 2019

AbstractLateral meningocele syndrome (LMS), a genetic disorder characterized by meningoceles and skeletal abnormalities, is associated with NOTCH3 mutations. We created a mouse model of LMS (Notch3tm1.1Ecan) by introducing a tandem termination codon in the Notch3 locus upstream of the proline (P), glutamic acid (E), serine (S) and threonine (T) domain. Microcomputed tomography demonstrated that Notch3tm1.1Ecan mice exhibit osteopenia. The cancellous bone osteopenia was no longer observed after the intraperitoneal administration of antibodies directed to the negative regulatory region (NRR) of Notch3. The anti‐Notch3 NRR antibody suppressed the expression of Hes1, Hey1, and Hey2 (Notch target genes), and decreased Tnfsf11 (receptor activator of NF Kappa B ligand) messenger RNA in Notch3tm1.1Ecan osteoblast (OB) cultures. Bone marrow‐derived macrophages (BMMs) from Notch3tm1.1Ecan mutants exhibited enhanced osteoclastogenesis in culture, and this was increased in cocultures with Notch3tm1.1Ecan OB. Osteoclastogenesis was suppressed by anti‐Notch3 NRR antibodies in Notch3tm1.1Ecan OB/BMM cocultures. In conclusion, the cancellous bone osteopenia of Notch3tm1.1Ecan mutants is reversed by anti‐Notch3 NRR antibodies.
Identification of genes and pathways in human antigen‐presenting cell subsets in response to polio vaccine by bioinformatical analysis
Authors: Yanan Wu , Wenyong Zhu , Ze Liu , Jingxia Gao , Lei Ma , Shengjie Ouyang , Bingjie Zhang , Xiaohu Dai , Weidong Li , Guoyang Liao 
Published: June 2019

AbstractBackgroundPolio eradication has been achieved in the world except for three countries due to the widespread use of the inactivated poliovirus vaccine (IPV) and the live‐attenuated oral poliovirus vaccine. Following polio eradication, the IPV would be the only polio vaccine available. However, the mechanisms of the interactions between IPV and human antigen‐presenting cells (APCs) remain largely unclear.MethodsTo investigate the involvement of the IPV in human monocytes, we downloaded the gene chip GSE44721 from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified using the GEO2R analysis tool. Functional and pathway enrichment analyses were performed for DEGs using the Metascape database. DEG‐associated protein–protein‐interactions (PPIs) were established by the Search Tool for the Retrieval of Interacting Genes website and visualized by Cytoscape.ResultsThere were 240 DEGs (51 upregulated and 189 downregulated genes) identified from the GSE44721 data set, and they were significantly enriched in several biological processes, including antigen processing and presentation of lipid antigen via MHC class Ib, adaptive immune response, and response to interferon‐gamma. One hundred thirty‐six nodes were screened from the DEG PPI network. There were six significant hub proteins (WDR36, MRTO4, RPF2, PPAN, CD40, and BMS1) that regulated the IPV in human monocytes.ConclusionsIn summary, using bioinformatical analysis, we have information for the immunization activated by the IPV in monocytes. Moreover, hormones and cytokines regulate the activation of APCs.
Advantages of asymmetrical over symmetrical cells for detection of under deposit CO2 corrosion using electrochemical noise
Authors: Zeidabadinejad Mahdieh , Shahidi‐Zandi Mehdi , Foroughi Mohammad Mehdi , Asadollahzadeh Hamideh 
Published: June 2019

Electrochemical noise (EN) measurements were done on symmetrical and asymmetrical cells after immersion in 3.5% NaCl solutions saturated with CO2 and containing different concentrations of sodium bicarbonate to get a better interpretation of the under‐deposit corrosion of mild steel. The symmetrical cell was constructed from two sand‐deposited electrodes and the asymmetrical cell was prepared from one sand‐deposited electrode and one bare electrode. The combination of the standard deviation of partial signal (SDPS) plots arising from wavelet analysis and recurrence plots for analyzing the EN signals obtained from symmetrical and asymmetrical cells confirmed the higher detective ability of the asymmetrical cells in comparison with the symmetrical cells for measuring the under‐deposit corrosion of mild steel. Both the SDPS and recurrence plots arising from symmetrical cells showed no significant change in the corrosion severity of the steel alloy with adding sodium bicarbonate. In contrast, the SDPS and recurrence plots of the asymmetrical cells proved that the corrosion severity decreased initially with adding the bicarbonate and thereafter remained constant with increasing the bicarbonate concentration. The optical microscopy images of the sand‐covered electrode surface confirmed the latter result.