bluuebunny/crossref_metadata_2025_split huggingface dataset:
Costos directos del cuidado paliativo domiciliario para pacientes con cáncer gástrico estadio IV en Colombia*
Authors: León Martha Ximena 
Published: August 2018

En Latinoamérica se han publicado pocos estudios acerca de la efectividad y costos del cuidado paliativo, y ello puede constituir una barrera para la implementación del cuidado paliativo (CP). Objetivo: Determinar la frecuencia de uso y costos directos relacionados con los servicios de CP domiciliario para pacientes con cáncer gástrico estadio IV en Colombia. Métodos: Estudio descriptivo de análisis de costos. Revisión de literatura para identificar síntomas, tratamientos y servicios más comunes en pacientes con cáncer gástrico estadio IV. Posteriormente, se construyó un borrador de instrumento. Usando un proceso Delphi modificado, nueve expertos en cuidado paliativo revisaron y sugirieron cambios en el instrumento. Se incluyeron 83 historias clínicas de pacientes afiliados a tres aseguradoras en el estudio. Criterios de inclusión: pacientes con cáncer gástrico estadio IV que estuvieran recibiendo CP en casa entre julio y diciembre de 2013. De acuerdo con los resultados del instrumento y un abordaje bottom up, se realizó un análisis de costos para cada caso. Resultados: El promedio de estancia en el programa fue de 37,6 días con un costo promedio de 60 dólares paciente/ día y de 979 dólares total/paciente (rango:77-4647 dólares). El porcentaje de distribución del costo total fue: 55 % visitas domiciliarias, 36 % medicamentos, 8 % procedimientos, y 1 % exámenes paraclínicos. El costo promedio día fue de 59,88 dólares. Conclusiones: Teniendo en cuenta los costos relacionados, el CP en casa podría ser una asignación apropiada de recursos en salud con beneficios potenciales para los pacientes.
Caracterización de los pacientes, tratamiento y complicaciones más frecuentes de los recién nacidos con gastrosquisis y onfalocele manejados en la Unidad de Recién Nacidos del Hospital Universitario San Ignacio. Experiencia de 10 años
Authors: Bertolotto Ana María , Córdoba Maria Adelaida , Vargas Vaca Yaris Anzully , Guzmán Cruz Paula Carolina , Álvarez Angélica Natalia 
Published: August 2018

Introducción: Los defectos de la pared abdominal son infrecuentes; por ello se debe optimizar su diagnóstico y manejo, identificando las características clínicas más frecuentes. Objetivo: Caracterizar la población de pacientes con gastrosquisis y onfalocele atendidos en el Hospital Universitario San Ignacio (HUSI) en los últimos 10 años. Métodos: Se revisaron todas las historias de pacientes con defectos de pared abdominal atendidos en la Unidad de ReciénNacidos (URN) del HUSI entre el 2004 y el2014. Se estimaron las frecuencias de los hallazgosmás relevantes. Resultados: Se evaluaron 29pacientes, 18 pacientes con gastrosquisis (62 %)y 11 con onfalocele (38 %). Se encontró asociacióncon otras malformaciones en 20 pacientes(68,9 %) y en 5 casos de pacientes con onfalocelese evidenció cromosomopatías (17,2 %). Todoslos pacientes con gastrosquisis, y el 37 % de losniños con onfalocele recibieron manejo quirúrgico;el 55 %, manejo médico, y el 8 %, manejocombinado. El tiempo promedio de ayuno fue de11,8 días y con nutrición parenteral fue de 19,8días. No se encontró asociación con el consumomaterno de sustancias psicoactivas. Conclusión:Los defectos de pared son una condición rara querequirió un manejo especializado en la URN delHUSI durante los años 2004-2014. Fueron atendidos29 pacientes con características y evoluciónclínica similar a la reportada en la literaturalatinoamericana, aunque con tiempo de ayuno yde nutrición parenteral menor. No se encontróasociación con el consumo materno de sustanciaspsicoactivas.
Caracterización del estudiante de medicina de la Pontificia Universidad Javeriana de Cali. Aspectos sociodemográficos
Authors: Moreno Sandra , Vergara Carlos , Murcia Camila , Moreno Freddy 
Published: August 2018

Introducción: En el 2010, la Pontificia Universidad Javeriana Cali (PUJ Cali) ofertópor primera vez la carrera de Medicina. Debido a que el programa académico esrelativamente nuevo y ofrece un plan de estudios innovador, basado en un currículo porcompetencias que integra las ciencias básicas, las clínicas médicas y la salud pública,es necesario verificar que el perfil de ingreso propuesto de los neojaverianos se adaptea las exigencias académicas, científicas y humanas requerida en la formación de losmédicos javerianos. Objetivo: Caracterizar a los estudiantes del programa de medicinade la PUJ Cali, a partir de aspectos sociodemográficos. Materiales y métodos: Estudiodescriptivo de corte transversal que caracterizó a los estudiantes de Medicina de laPUJ Cali. De los 423 estudiantes matriculados en el primer periodo (enero-junio) de2013, 299 diligenciaron una encuesta diseñada en el software Cardiff TELEform®versión 10.0. Resultados: De los 299 estudiantes que diligenciaron la encuesta, el64,3 % son mujeres y el 35,7 % son hombres; el 90,9 % son mayores de 18 años; el59 % nacieron en la ciudad de Cali. La vivienda se distribuye en el 69,9 % en losestratos 4, 5 y 6. Conclusión: La muestra estudiada corresponde a una población homogénea donde un poco más de la mitad delos estudiantes nacieron y residen en la ciudadde Cali. Asimismo, los estratos socioeconómicospredominantes son el 4, 5 y 6, teniendo en cuentaque una cantidad considerable de estudiantesdesconocen el estrato donde residen.
Técnica de diafanización para describir el desarrollo embrionario del sistema óseo. Revisión de la literatura
Authors: Sandoval David , Tellez Jelua , Rivera Guillermo , Moreno Sandra , Moreno Freddy 
Published: August 2018

Introducción: La técnica anatómica de diafanización consiste en transparentar los tejidosblandos de organismos vertebrados (aclaramiento), para teñir los tejidos mineralizadosy poder visualizar los componentes óseos y cartilaginosos (tinción). Objetivo: Revisarlos reportes disponibles en la literatura especializada que han descrito el desarrolloembrionario del sistema óseo de mamíferos a través de técnicas de diafanización ytinción simple o tinción doble. Materiales y métodos: Se revisó la literatura sobreel tema en PubMed, Google Académico y SciELO, basándose en la metodologíaPRISMA, a través de la asociación del término double staining con los descriptores ensalud del Medical Subject Headings (MeSH) alizarin red, alcian blue, bone y cartilage,y la combinación con los operadores boleanos + y &. Resultados: En esta revisión dela literatura se incluyeron 22 artículos que describieron la técnica de diafanizacióny tinción simple o tinción doble empleada en la observación, registro y análisis del desarrollo embrionario del sistema óseo de mamíferos.Conclusión: La diafanización y tinciónsimple o tinción doble es una técnica anatómicade estudio —versátil y de bajo costo— del desarrolloembrionario del sistema óseo, la cualpuede emplearse en estudios toxicológicos, paradescartar la posibilidad de anomalías de desarrollodurante la formación del cartílago (condrogénesis)y del hueso (osteogénesis), asociadas a laexposición de un posible agente teratógeno.
Blood Flow Restriction Therapy Following Microfracture Surgery for Osteochondritis Dissecans in a Collegiate Athlete
Authors: Kruithof Emily E. , Thomas Spencer A. , Tripp Patricia 
Published: August 2018

A 19-year-old college football wide receiver (height: 193 cm, mass: 94 kg) reported sudden onset medial pain in his right knee during practice. Diagnostic imaging revealed medial femoral condyle osteochondral defects with loose bodies and a grade I medial collateral sprain (MCL). The medical and therapeutic intervention included arthroscopic microfracture, chondroplasty, loose body removal, and a 6-month rehabilitation program that included the use of blood flow restriction therapy. Current evidence estimates a 7–11-month recovery following microfracture surgery for the management of articular cartilage lesions, with a return-to-sport rate of only 50–60%.
Educating Parents of Children in Sport About Abuse Using Narrative Pedagogy
Authors: McMahon Jenny , Knight Camilla J. , McGannon Kerry R. 
Published: August 2018

Research on abuse in sport reveals that sporting environments are unique contexts where athlete abuse can occur. An international panel on “safe sport” identified the need to implement strategies to ensure sport is safe for all. One strategy identified as a way of preventing abuse from occurring in sport is to educate the parents of athletes. This study centres on an education intervention implemented with 14 parents from a gymnastics and swimming context where narrative pedagogy (e.g., athletes’ stories of abuse) was used. As a result of engaging with narrative pedagogy, parents were able to identify unacceptable coaching practices. However, the extent of several dominant cultural ideologies (e.g., competitive performance ideology) became known through the parents’ responses and influenced the way they took up the athletes’ abuse stories.
Sporting Shanghai: Haipai Cosmopolitanism, Glocal Cityness, and Urban Policy as Mega-Event
Authors: Yu Lin , Xue Hanhan , Newman Joshua I. 
Published: August 2018

In recent years, Shanghai has become one of Asia’s major players in the bidding for, and hosting of, international sporting events. Uniquely positioned by history (e.g., China’s liberalized urban node to the globalizing economy, an imbedded urban cosmopolitanism) and geopolitics (e.g., a shift toward free market domestic political economy, a growing pro-corporatist governing alliance), sporting Shanghai provides a critical site of analysis for contemporary sport-based global-localisms. In this study, we examine how local culture, global commercialism, and policy discourse intersect to “produce” the global sporting city. We conclude with a discussion of how sport in this context is manufactured not only as economic, social, and political catalyst, but also how sport policy itself represents a conjunctural city as “modernization” event.
Draft Genome Sequences of 112 Salmonella enterica Serovar Dublin Strains Isolated from Humans and Animals in Brazil
Authors: Campioni Fábio , Vilela Felipe Pinheiro , Cao Guojie , Kastanis George , Miller Daniela , Sanchez Leon Maria , Tiba-Casas Monique Ribeiro , Fernandes Sueli Aparecida , Rodrigues Dália dos Prazeres , Costa Renata Garcia , Allard Marc William , Falcão Juliana Pfrimer 
Published: August 2018

ABSTRACT

Salmonella enterica
serovar Dublin is a strongly adapted serovar that causes enteritis and/or systemic disease in cattle and results in high rates of mortality. Here, we report the draft genome sequences of 112
S.
Dublin strains isolated from humans and animals in Brazil. These draft genome sequences will help enhance our understanding of this serovar in Brazil.
Structure and Genome Organization of a Novel Fiji Strain of Sweet Potato Vein Clearing Virus Identified by High-Throughput Sequencing
Authors: Wu Liping , Liu Huawei , Abad Jorge , French Ronald D. , Li Ruhui 
Published: August 2018

ABSTRACT

The complete genome of a
Sweet potato vein clearing virus
(SPVCV) isolate infecting a quarantined sweet potato accession from Fiji was determined. Sequence comparisons revealed the highest nucleotide sequence identity of 94.6% with that of the SPVCV type species, an isolate from the Dominican Republic. The virus was mechanically transmitted to
Nicotiana bigelovii
plants.
Complete Genome Sequence of a Novel Mutant Strain of Vibrio parahaemolyticus from Pacific White Shrimp (Penaeus vannamei)
Authors: Kanrar Siddhartha , Dhar Arun K. 
Published: August 2018

ABSTRACT

The acute hepatopancreatic necrosis disease (AHPND) of
Penaeus vannamei
shrimp is caused by
Vibrio parahaemolyticus
carrying toxin genes,
pirA
and
pirB
. We report the complete genome sequence of the novel
V. parahaemolyticus
strain R14, which did not display AHPND symptoms in
P. vannamei
despite containing the binary toxin genes.
Two Novel, Distantly Related Papillomaviruses Isolated from Healthy Skin of the Timor Deer (Rusa timorensis)
Authors: Mengual-Chuliá Beatriz , Wibbelt Gudrun , Gottschling Marc , Bravo Ignacio G. 
Published: August 2018

ABSTRACT

We report the complete genome sequences of
Rusa timorensis papillomavirus 1
(RtimPV1) and
Rusa timorensis papillomavirus 2
(RtimPV2), isolated from hair follicles of asymptomatic skin from the same Timor deer specimen. RtimPV1 and RtimPV2 are evolutionarily only distantly related. RtimPV1 lacks a canonical E2-binding site, and RtimPV2 does not carry an
E6
gene.
Complete Genome Sequence of Campylobacter jejuni Strain 12567, a Livestock-Associated Clade Representative
Authors: Sacher J. C. , Yee E. , Szymanski C. M. , Miller W. G. 
Published: August 2018

ABSTRACT

We report here the complete genome sequence of
Campylobacter jejuni
strain 12567, a member of a
C. jejuni
livestock-associated clade that expresses glycoconjugates associated with improved gastrointestinal tract persistence.
Draft Genome Sequence of Moorella sp. Strain Hama-1, a Novel Acetogenic Bacterium Isolated from a Thermophilic Digestion Reactor
Authors: Harada Jun , Yamada Takeshi , Giri Surya , Hamada Masako , Nobu Masaru K. , Narihiro Takashi , Tsuji Hideto , Daimon Hiroyuki 
Published: August 2018

ABSTRACT

Moorella
sp. strain Hama-1 was isolated from a thermophilic anaerobic digestion reactor treating poly(
l
-lactic acid). The strain is a thermophilic acetogen capable of lactate oxidation under anaerobic conditions. Here, we report the draft genome sequence of strain Hama-1, comprising 3.27 Mb in 48 contigs, with a G+C content of 56.6%.
Draft Genome Sequence of Fish Pathogen Aeromonas bestiarum GA97-22
Authors: Kumru Salih , Tekedar Hasan C. , Griffin Matt J. , Waldbieser Geoffrey C. , Liles Mark R. , Sonstegard Tad , Schroeder Steven G. , Lawrence Mark L. , Karsi Attila 
Published: August 2018

ABSTRACT

Aeromonas bestiarum
is a Gram-negative mesophilic motile bacterium causing acute hemorrhagic septicemia or chronic skin ulcers in fish. Here, we report the draft genome sequence of
A. bestiarum
strain GA97-22, which was isolated from rainbow trout in 1997. This genome sequence will improve our understanding of the complex taxonomy of motile aeromonads.
Complete Genome Sequence of Dengue Virus Serotype 2, Asian/American Genotype, Isolated from the Urine of a Venezuelan Child with Hemorrhagic Fever in 2016
Authors: Blohm Gabriela M. , Paniz-Mondolfi Alberto E. , Márquez Marilianna C. , Loeb Julia C. , Pacheco Carlos , Lednicky John A. , Pulliam Juliet R. C. , Morris J. Glenn 
Published: August 2018

ABSTRACT

The complete genome sequence was obtained for a
Dengue virus
2 isolate from the urine of an 8-year-old girl who was hospitalized with dengue hemorrhagic fever in 2016 in Venezuela.
T Cell Responses to Nonstructural Protein 3 Distinguish Infections by Dengue and Zika Viruses
Authors: Herrera Bobby Brooke , Tsai Wen-Yang , Brites Carlos , Luz Estela , Pedroso Celia , Drexler Jan Felix , Wang Wei-Kung , Kanki Phyllis J. 
Published: August 2018

ABSTRACTThe 2015–2016 Zika virus (ZIKV) epidemic in the Americas and the Caribbean demonstrated that clinical assays to detect, distinguish, and characterize immune responses to flaviviral infections are needed. ZIKV and dengue virus (DENV) are mosquito-transmitted flaviviruses sharing overlapping geographic distributions and have significant sequence similarities that can increase the potential for antibody and T cell cross-reaction. Using nonstructural protein 1-based enzyme-linked immunosorbent assays (ELISAs), we determined the serostatus of individuals living in a region of DENV and ZIKV endemicity in Brazil, identifying individuals with primary DENV (pDENV) and primary ZIKV (pZIKV), ZIKV with primary DENV (ZIKVwpDENV), and secondary DENV (sDENV) infections; the presence of pDENV and pZIKV was further confirmed by neutralization tests. Development of an enzyme-linked immunosorbent spot (ELISPOT) assay for DENV and ZIKV structural and nonstructural (NS) protein antigens enabled us to distinguish infections by these viruses based on T cell responses and to characterize those responses. We found that gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) T cell responses to NS3 differentiated DENV and ZIKV infections with 94% sensitivity and 92% specificity. In general, we also showed that pDENV and sDENV cases and pZIKV and ZIKVwpDENV cases elicit similar T cell response patterns and that HIV-infected individuals show T cell responses that are lower than those shown by HIV-negative individuals. These results have important implications for DENV and ZIKV diagnostic and vaccine development and provide critical insights into the T cell response in individuals with multiple flaviviral infections.IMPORTANCEThe potential for antibody and T cell cross-reactions to DENV and ZIKV, flaviviruses that cocirculate and can sequentially infect individuals, has complicated diagnostic and vaccine development. Our serological data show that antibodies to nonstructural protein 1 can distinguish sequential human infections by DENV and ZIKV. The development of a simple and inexpensive assay also enables the differentiation of DENV and ZIKV infections based on characterization of T cell responses. Our T cell data reveal strong response patterns that are similar in nature to those seen with individuals with one or multiple DENV infections and with individuals with only primary ZIKV infection and ZIKV-infected individuals with previous DENV exposure. The characterization of T cell responses in a serologically validated group of individuals is of relevance to the development of vaccines and immunotherapeutics against these global threats.
Relative Contributions of
Halobacteriovorax
and Bacteriophage to Bacterial Cell Death under Various Environmental Conditions
Authors: Chen Huan , Laws Edward A. , Martin Julio L. , Berhane Timkhite-Kulu , Gulig Paul A. , Williams Henry N. 
Published: August 2018

ABSTRACT

The role of protists and bacteriophages in bacterial predation in the microbial food web has been well studied. There is mounting evidence that
Bdellovibrio
and like organisms (BALOs) also contribute to bacterial mortality and, in some cases, more so than bacteriophages. A full understanding of the ecologic function of the microbial food web requires recognition of all major predators and the magnitude of each predator’s contribution. Here we investigated the contribution of
Halobacteriovorax
, one of the BALOs, and bacteriophages when incubated with their common prey,
Vibrio vulnificus
, in a seawater microcosm. We observed that
Halobacteriovorax
was the greatest responder to the prey, increasing 18-fold with a simultaneous 4.4-log-unit reduction of
V. vulnificus
at 40 h, whereas the bacteriophage population showed no significant increase. In subsequent experiments to formulate a medium that would support the predatory activities and replication of both predators, low-nutrient media favored the predation and replication of the
Halobacteriovorax
, whereas higher-nutrient media enhanced phage growth. The greatest prey reduction and replication of both
Halobacteriovorax
and phage were observed in media with moderate nutrient levels. Additional experiments show that the predatory activities of both predators were influenced by environmental conditions, specifically, temperature and salinity. The two predators combined exerted greater control on
V. vulnificus
, a synergism that may be exploited for practical applications to reduce bacterial populations. These findings suggest that along with bacteriophage and protists,
Halobacteriovorax
has the potential to have a prominent role in bacterial mortality and cycling of nutrients, two vital ecologic functions.

IMPORTANCE
Although much has been reported about the marine microbial food web and the role of micropredators, specifically viruses and protists, the contribution of
Bdellovibrio
-like predators has largely been ignored, posing a major gap in understanding food web processes. A complete scenario of the microbial food web cannot be developed until the roles of all major micropredators and the magnitude of their contributions to bacterial mortality, structuring of microbial communities, and cycling of nutrients are assessed. Here we show compelling evidence that
Halobacteriovorax
, a predatory bacterium, is a significant contributor to bacterial death and, in some cases, may rival viruses as agents of bacterial mortality. These results advance current understanding of the microbial loop and top-down control on the bacterial community.
Cyclic Enterobacterial Common Antigen Maintains the Outer Membrane Permeability Barrier of Escherichia coli in a Manner Controlled by YhdP
Authors: Mitchell Angela M. , Srikumar Tharan , Silhavy Thomas J. 
Published: August 2018

ABSTRACT

Gram-negative bacteria have an outer membrane (OM) impermeable to many toxic compounds that can be further strengthened during stress. In
Enterobacteriaceae
, the envelope contains enterobacterial common antigen (ECA), a carbohydrate-derived moiety conserved throughout
Enterobacteriaceae
, the function of which is poorly understood. Previously, we identified several genes in
Escherichia coli
K-12 responsible for an RpoS-dependent decrease in envelope permeability during carbon-limited stationary phase. For one of these,
yhdP
, a gene of unknown function, deletion causes high levels of both vancomycin and detergent sensitivity, independent of growth phase. We isolated spontaneous suppressor mutants of
yhdP
with loss-of-function mutations in the ECA biosynthesis operon. ECA biosynthesis gene deletions suppressed envelope permeability from
yhdP
deletion independently of envelope stress responses and interactions with other biosynthesis pathways, demonstrating suppression is caused directly by removing ECA. Furthermore,
yhdP
deletion changed cellular ECA levels and
yhdP
was found to co-occur phylogenetically with the ECA biosynthesis operon. Cells make three forms of ECA: ECA lipopolysaccharide (LPS), an ECA chain linked to LPS core; ECA phosphatidylglycerol, a surface-exposed ECA chain linked to phosphatidylglycerol; and cyclic ECA, a cyclized soluble ECA molecule found in the periplasm. We determined that the suppression of envelope permeability with
yhdP
deletion is caused specifically by the loss of cyclic ECA, despite lowered levels of this molecule found with
yhdP
deletion. Furthermore, removing cyclic ECA from wild-type cells also caused changes to OM permeability. Our data demonstrate cyclic ECA acts to maintain the OM permeability barrier in a manner controlled by YhdP.

IMPORTANCE
Enterobacterial common antigen (ECA) is a surface antigen made by all members of
Enterobacteriaceae
, including many clinically relevant genera (e.g.,
Escherichia
,
Klebsiella
,
Yersinia
). Although this surface-exposed molecule is conserved throughout
Enterobacteriaceae
, very few functions have been ascribed to it. Here, we have determined that the periplasmic form of ECA, cyclic ECA, plays a role in maintaining the outer membrane permeability barrier. This activity is controlled by a protein of unknown function, YhdP, and deletion of
yhdP
damages the OM permeability barrier in a cyclic ECA-dependent manner, allowing harmful molecules such as antibiotics into the cell. This role in maintenance of the envelope permeability barrier is the first time a phenotype has been described for cyclic ECA. As the Gram-negative envelope is generally impermeable to antibiotics, understanding the mechanisms through which the barrier is maintained and antibiotics are excluded may lead to improved antibiotic delivery.