bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic significance of miR-122 expression after curative resection in patients with hepatocellular carcinoma
Authors: Ha Sang Yun , Yu Jeong Il , Choi Changhoon , Kang So Young , Joh Jae-Won , Paik Seung Woon , Kim Seonwoo , Kim Minji , Park Hee Chul , Park Cheol-Keun 
Published: October 2019

AbstractDownregulation of MicroRNA-122 (miR-122) and its association with cancer progression have been reported in hepatocellular carcinoma (HCC) cell line models and a limited number of HCC samples. Recently, restoration of miR-122 expression by direct delivery of miR-122 yielded promising results in HCCs. However, the prognostic effect of miR-122 expression in human HCC samples is not fully understood. We investigated the expression level of miR-122 by quantitative real-time polymerase chain reaction in 289 curatively resected HCC samples and 20 normal liver samples and evaluated the prognostic effect of miR-122 expression. The relative quantification value of miR-122 was much lower in HCC samples than in normal liver tissues. During a median 119 months of follow-up for survival, the low miR-122 expression group showed shorter recurrence-free survival (RFS) (p = 0.033) and intrahepatic recurrence-free survival (IHRFS) (p = 0.014), and a trend of short distant metastasis-free survival (DMFS) (p = 0.149) than high expression group. On multivariate analysis, miR-122 expression was an independent prognostic factor for RFS, IHRFS and DMFS. Downregulation of miR-122 expression, frequently found in HCC samples, was an independent prognostic factor for RFS after curative resection. Emerging therapeutic approaches targeting miR-122 could be applicable in patients with miR-122 downregulated hepatocellular carcinoma.
Development of two different formats of heterogeneous fluorescence immunoassay for bioanalysis of afatinib by employing fluorescence plate reader and KinExA 3200 immunosensor
Authors: Darwish Ibrahim A. , AlRabiah Haitham , Hamidaddin Mohammed A. 
Published: October 2019

AbstractAfatinib (AFT) is a potent and highly selective drug used to treat various solid tumors including non-small cell lung cancer (NSCLC). To ensure safe and effective treatment of cancer patients with AFT, its plasma concentrations should be monitored. Thus, sensitive immunoassays are required for measuring AFT concentrations in plasma samples. In this study, two different formats of heterogeneous fluorescent immunoassays were developed and validated for AFT bioanalysis. These assays were microwell-based fluorescence immunoassay (FIA) using fluorescence plate reader and kinetic exclusion assay (KinExA) using KinExA 3200 immunosensor. Both FIA and KinExA were developed using the same reagents: mouse anti-AFT antibody, solid-phase immobilized AFT conjugated with bovine serum albumin protein (AFT-BSA), and goat anti-mouse IgG labelled with fluorescein isothiocyanate (FITC-IgG) for signal generation. The analytical performances of both assays were comparatively evaluated, and the results revealed that although both assays had comparable accuracies, KinExA was superior to FIA in terms of sensitivity and precisions. Moreover, both FIA and KinExA were better alternatives to the existing chromatographic methods for bioanalysis of AFT. The proposed FIA and KinExA are anticipated to effectively contribute in ensuring safe and effective treatment with AFT in clinical settings.
Impact of vertical air motions on ice formation rate in mixed-phase cloud layers
Authors: Bühl Johannes , Seifert Patric , Engelmann Ronny , Ansmann Albert 
Published: October 2019

AbstractThe relationship between vertical air velocity at cloud base and primary ice formation has been measured for shallow mixed-phase cloud layers (thickness <380 m) by means of ground-based cloud radar and Doppler lidar. For layers with a cloud-top temperature below −12 °C, an increase of vertical-velocity standard deviation from 0.1 to 1.0 m s−1 leads to an increase in the mass flux of ice water by two orders of magnitude. The cloud layers under study were selected in such a way that secondary ice-formation processes played a minor role, and primary ice formation was the dominant source of ice formation. Phenomenological parameterizations of the ice mass and the ice mass flux as functions of standard deviation of vertical air velocity are given.
Pyridoxamine-phosphate oxidases and pyridoxamine-phosphate oxidase-related proteins catalyze the oxidation of 6-NAD(P)H to NAD(P)+
Authors: Marbaix Alexandre Y. , Chehade Georges , Noël Gaëtane , Morsomme Pierre , Vertommen Didier , Bommer Guido T. , Van Schaftingen Emile 
Published: October 2019

Abstract
6-NADH and 6-NADPH are strong inhibitors of several dehydrogenases that may form spontaneously from NAD(P)H. They are known to be oxidized to NAD(P)+ by mammalian renalase, an FAD-linked enzyme mainly present in heart and kidney, and by related bacterial enzymes. We partially purified an enzyme oxidizing 6-NADPH from rat liver, and, surprisingly, identified it as pyridoxamine-phosphate oxidase (PNPO). This was confirmed by the finding that recombinant mouse PNPO oxidized 6-NADH and 6-NADPH with catalytic efficiencies comparable to those observed with pyridoxine- and pyridoxamine-5′-phosphate. PNPOs from Escherichia coli, Saccharomyces cerevisiae and Arabidopsis thaliana also displayed 6-NAD(P)H oxidase activity, indicating that this ‘side-activity’ is conserved. Remarkably, ‘pyridoxamine-phosphate oxidase-related proteins’ (PNPO-RP) from Nostoc punctiforme, A. thaliana and the yeast S. cerevisiae (Ygr017w) were not detectably active on pyridox(am)ine-5′-P, but oxidized 6-NADH, 6-NADPH and 2-NADH suggesting that this may be their main catalytic function. Their specificity profiles were therefore similar to that of renalase. Inactivation of renalase and of PNPO in mammalian cells and of Ygr017w in yeasts led to the accumulation of a reduced form of 6-NADH, tentatively identified as 4,5,6-NADH3, which can also be produced in vitro by reduction of 6-NADH by glyceraldehyde-3-phosphate dehydrogenase or glucose-6-phosphate dehydrogenase. As 4,5,6-NADH3 is not a substrate for renalase, PNPO or PNPO-RP, its accumulation presumably reflects the block in the oxidation of 6-NADH. These findings indicate that two different classes of enzymes using either FAD (renalase) or FMN (PNPOs and PNPO-RPs) as a cofactor play an as yet unsuspected role in removing damaged forms of NAD(P).
Asteroseismology of evolved stars to constrain the internal transport of angular momentum
Authors: Eggenberger P. , den Hartogh J. W. , Buldgen G. , Meynet G. , Salmon S. J. A. J. , Deheuvels S. 
Published: October 2019

Context. Asteroseismic observations enable the characterisation of the internal rotation of evolved stars. These measurements reveal that an unknown efficient angular momentum (AM) transport mechanism is needed for subgiant and red giant stars in addition to hydrodynamic transport processes. A revised prescription for AM transport by the magnetic Tayler instability has been recently proposed as a possible candidate for such a missing mechanism.
Aims. We compare the rotational properties predicted by this magnetic AM transport to asteroseismic constraints obtained for evolved stars with a particular focus on the subgiant phase.
Methods. We computed models accounting for the recent prescription for AM transport by the Tayler instability with the Geneva stellar evolution code for subgiant and red giant stars, for which an asteroseismic determination of both core and surface rotation rates is available.
Results. The revised prescription for the transport by the Tayler instability leads to low core rotation rates after the main sequence that are in better global agreement with asteroseismic measurements than those predicted by models with purely hydrodynamic processes or with the original Tayler-Spruit dynamo. A detailed comparison with asteroseismic data shows that the rotational properties of at most two of the six subgiants can be correctly reproduced by models accounting for this revised magnetic transport process. This result is obtained independently of the value adopted for the calibration parameter in this prescription. We also find that this transport by the Tayler instability faces difficulties in simultaneously reproducing asteroseismic measurements available for subgiant and red giant stars. The low values of the calibration parameter needed to correctly reproduce the rotational properties of two of the six subgiants lead to core rotation rates during the red giant phase that are too high. Inversely, the higher values of this parameter needed to reproduce the core rotation rates of red giants lead to a very low degree of radial differential rotation before the red giant phase, which is in contradiction with the internal rotation of subgiant stars.
Conclusions. In its present form, the revised prescription for the transport by the Tayler instability does not provide a complete solution to the missing AM transport revealed by asteroseismology of evolved stars.
Nasale mukoziliäre Clearance und
Vitamin-D-Mangel
Authors: Haxel Boris 
Published: October 2019

Akcan FA et al. Evaluation of nasal mucociliary clearance time in patients with
Vitamin-D deficiency. Eur Arch Otorhinolaryngol 2019; doi:
10.1007/s00405–019–05286-y
HNO-Ärzte aus der Türkei untersuchten die Zeitdauer der nasalen mukoziliären
Clearance (NMC) bei Patienten mit Vitamin-D-Mangel.
Ausbildungscurriculum zum Zertifikat „Diagnostik und
Therapie oropharyngealer Dysphagien, inklusive FEES“ der Deutschen Gesellschaft
für Phoniatrie und Pädaudiologie und der Deutschen Gesellschaft für
Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie
Authors: Graf Simone , Keilmann Annerose , Dazert Stefan , Deitmer Thomas , Stasche Norbert , Arnold Barbara , Löhler Jan , Arens Christoph , Pflug Christina 
Published: October 2019

Zusammenfassung
Hintergrund In Deutschland leiden ca. 5 Millionen Menschen jeden
Lebensalters an einer Dysphagie. Durch den demografischen Wandel und die
verbesserte medizinische Versorgung ist mit einer Zunahme der Inzidenz von
Schluckstörungen zu rechnen. Die Dysphagie ist mit einer erhöhten Morbiditäts-
und Mortalitätsrate assoziiert und führt zu einer erheblichen finanziellen
Belastung der Gesundheitssysteme. Die zwei häufigsten Ursachen einer
Schluckstörung sind neurologische Erkrankungen und Kopf-Hals-Tumore. Die
Diagnostik und Therapie haben sich in den letzten Jahrzehnten kontinuierlich
weiterentwickelt. Insbesondere die flexibel-endoskopische Beurteilung des
Schluckaktes (FEES) ist zu einem festen Bestand in der Abklärung von
Schluckstörungen geworden.
Ergebnisse Das Zertifikat „Diagnostik und Therapie oropharyngealer
Dysphagien, inkl. FEES“ wurde von der Deutschen Gesellschaft für Phoniatrie und
Pädaudiologie (DGPP) und der Deutschen Gesellschaft für
Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie (DGHNO KHC) in
Zusammenarbeit mit dem Deutschen Berufsverband für Phoniatrie und Pädaudiologie
und dem Deutschen Berufsverband der Hals-Nasen-Ohrenärzte entwickelt.Es besteht aus drei Teilen: den Modulen (A, B und C), der indirekten Supervision
und einer praktischen Prüfung. Aufbau, detaillierte Inhalte und die
Anforderungen zur Erlangung des Zertifikates werden im folgenden Artikel
beschrieben. Ebenso werden die Qualifikationen der Dozenten und Prüfer
festgesetzt.
Fazit Die systematische Ausbildung dient der Qualitätssicherung und
Etablierung von Standards in der Diagnostik und Therapie von oropharyngealen
Dysphagien in der Phoniatrie und Pädaudiologie und in der Hals-, Nasen-
Ohrenheilkunde.
Rheumatologische Rehabilitation und Begutachtung aus Patientensicht
Authors: Schmidt-Ohlemann Matthias 
Published: October 2019

ZusammenfassungIn der modernen Medizin spielt die Einbeziehung der Patientenperspektive eine zunehmende Rolle. Dies gilt in besonderem Maße für das System der Rehabilitation, in dem im SGB IX umfassende Patientenbeteiligungen und eine stärkere Personorientierung vorgesehen sind. Zur Praxis der medizinischen Rehabilitation werden Erwartungen, Hoffnungen und Erfahrungen von Patientinnen und Patienten mit entzündlich-rheumatischen Erkrankungen berichtet und Vorschläge zur Weiterentwicklung der medizinischen Rehabilitation für Rheumakranke unterbreitet. Im sozialrechtlichen Kontext werden im Rahmen der Verwaltungsverfahren häufig medizinische Unterlagen, Stellungnahmen und Gutachten benötigt. Probleme in den verschiedenen Verwaltungsverfahren werden aufgezeigt und die spezifisch rheumatologis chen Fragestellungen dargestellt. Vorschläge für eine sachgerechte und patientenorienterte Befunderstellung, sozialmedizinische Beurteilung und Begutachtung werden vorgestellt.
Newborn Screening for Congenital Hypothyroidism, Congenital Adrenal Hyperplasia, and Glucose-6-Phosphate Dehydrogenase Deficiency for Improving Health Care in India
Authors: Verma Jyotsna , Roy Papai , Thomas Divya C. , Jhingan Geetu , Singh Azad , Bijarnia-Mahay Sunita , Verma Ishwar C. 
Published: October 2019

AbstractNewborn screening (NBS) aims toward early detection of treatable congenital disorders. From January 2008 through December 2017, 13,376 newborns were screened for congenital hypothyroidism (CH), congenital adrenal hyperplasia (CAH), and glucose-6-phosphate dehydrogenase (G6PD) deficiency at Sir Ganga Ram Hospital, India, by measuring G6PD activity, thyroid-stimulating hormone, and 17-hydroxyprogesterone on dried blood specimens. The birth prevalence of 1:2,000 for CH, 1:2,500 for CAH, and 1:125 for G6PD deficiency indicates the latter as the most prevalent. Performance evaluation of testing reveals a robust screening program with 100% sensitivity and >99% specificity. Hence, we recommend NBS for early diagnosis and treatment to prevent adverse outcomes.
Work estimation and work fluctuations in the presence of non-ideal measurements
Authors: Debarba Tiago , Manzano Gonzalo , Guryanova Yelena , Huber Marcus , Friis Nicolai 
Published: October 2019

Abstract
From the perspective of quantum thermodynamics, realisable measurements cost work and result in measurement devices that are not perfectly correlated with the measured systems. We investigate the consequences for the estimation of work in non-equilibrium processes and for the fundamental structure of the work fluctuations when one assumes that the measurements are non-ideal. We show that obtaining work estimates and their statistical moments at finite work cost implies an imperfection of the estimates themselves: more accurate estimates incur higher costs. Our results provide a qualitative relation between the cost of obtaining information about work and the trustworthiness of this information. Moreover, we show that Jarzynski’s equality can be maintained exactly at the expense of a correction that depends only on the system’s energy scale, while the more general fluctuation relation due to Crooks no longer holds when the cost of the work estimation procedure is finite. We show that precise links between dissipation and irreversibility can be extended to the non-ideal situation.
Slaughtering of entire male pigs seen from the slaughterhouse perspective
Authors: Aaslyng M D , Støier S , Lund B W , Nielsen D B 
Published: October 2019

AbstractSeveral challenges need to be taken into account before a full transition to slaughtering entire male pigs. To optimize welfare, entire male pigs should spend as short a time as possible in the pick-up facilities at the farm. At the slaughterhouse, there is no need to control the ratio between genders in the pens, as this has only minor effects on welfare. Boar taint detection must be performed, and an analytical on-line method is being developed. Rejection limits for skatole and androstenone must be set as a balance between the risk of consumers getting a negative experience on consuming the meat and the number of carcasses discarded. Smoking is a promising strategy for use of the discarded carcasses, and as much smoke as possible is recommended. Using spices and serving the meat in complex meals can also be a strategy to utilize boar tainted meat. In addition to boar taint, meat from entire male pigs is less tender and the protein content is lower than meat from castrates. In addition, the weight distribution of the carcass is different, with entire males having approximately 500g more weight at the fore-end and less at the ham. However, the meat percentages are higher in the entire males, and this can somewhat counteract the weight distribution.
Sensory evaluation of meat and meat products: fundamentals and applications
Authors: Mörlein Daniel 
Published: October 2019

Abstract
Sensory analysis is widely applied to both fresh and processed meat. Herein, the frequently used sensory evaluation techniques are briefly characterised; some critical aspects, such as assessor selection, attribute generation, and context effects, are detailed. Without aiming to be exhaustive, some examples of previous research are show-cased to demonstrate what types of research questions can be answered by utilising sensory evaluation.
Antilisterial effect of juniper (Juniperus communis) and its mixed application with winter savory (Satureja montana) in beef protection
Authors: Nikolic B , Vasilijevic B , Mitic-Culafic D 
Published: October 2019

Abstract

Juniperus communis essential oil and post-distillation waste were tested for their antibacterial potential against common food contaminants. Results of microdilution assay directed further study of Listeria monocytogenes. Checkerboard assay showed synergistic antilisterial effect of both substances with conventional antibiotics. By evaluating of their cytotoxicity on human colon cells, post-distillation waste was denoted as selective against L. monocytogenes, being able to interfere with its in vitro adhesion on colon cells. On the other hand, the food preservative potential of J. communis essential oil was studied and compared with the activity of Satureja montana essential oil. In a microdilution assay, both substances induced antilisterial effect against reference ATCC 19111 strain and three wild isolates. A checkerboard assay showed synergism against isolates. An in vitro time-kill assay was used to confirm the types of interaction, and to estimate curve MIC values. Finally, in situ antilisterial efficacies of the individual essential oils and their mixture on red wine-marinated beef, previously inoculated with ATCC 19111 strain or primoisolate from beef carcass, were determined. All treatments enhanced the antilisterial potential of wine marinade. In conclusion, derivatives of J. communis possess significant antilisterial potential both in in vitro and in situ conditions, so further research is advised.
Food safety aspects of common carp produced in wastewaterfed fish ponds
Authors: Ljubojevic Pelic D , Pelic M , Djordjevic V , Cirkovic M 
Published: October 2019

Abstract
There is an increasing trend in the use of wastewater in fish production because wastewater-based aquaculture is a sustainable, biological way to treat and recycle wastewater. Different fish species including common carp have been reared in wastewater-fed ponds. However, untreated wastewater contains many kinds of contaminants that have adverse effects on human health and the environment. Thus, the health risks arising from fish produced in wastewater-filled fish ponds seem undeniable. On the other hand, the appropriate use of wastewater in aquaculture has important environmental and economic significance, including recycling nutrients and reuse of water. The main objective of the present review is verification of rearing fish in purified wastewater-fed fishponds through revision of available data related to fish meat safety. Wastewater could be an excellent source of nutrients for fish, but proper purification of this waste stream is necessary. Fish also have a role as bioindicators of the effectiveness of wastewater purification. Continuous monitoring of the presence and concentration of different contaminants in common carp and other fish species reared in purified wastewater is very important.
Modified atmosphere packaging of fish – an impact on shelf life
Authors: Babic Milijasevic J , Milijasevic M , Djordjevic V 
Published: October 2019

Abstract
The shelf life of fresh fish is short. Therefore, the fishing industry has always been willing to explore new technologies for shelf life extension. This paper will focus on one such technique, i.e., changing the nature of the gas surrounding the fish. The main purposes of modified atmosphere packaging of fish and fish products, but also other foodstuffs, are two-fold: to ensure the microbiological shelf life and the sensory quality of the product, including the colour, odour and palatability. During the last two decades, modified atmosphere packaging has become a dominant retail fish packaging technology. The main reasons for the development of modified atmosphere packaging are the continuous increase in fresh fish consumption, increases of urban populations and exhaustion of natural food resources. Developments in packaging materials and technologies have made the application of modified atmosphere packaging on a larger scale for fish and fish products feasible. In this paper, we present the basic principles of modified atmosphere packaging of fish, and the microbiological, sensory, chemical and physicochemical parameters that are important for the shelf life of fish packaged using this technology.
Household food waste in Belgrade - sin and unconcern
Authors: Janjic J , Popovic M , Radosavac A , Sarcevic D , Grbic S , Starcevic M , Baltic M Z 
Published: October 2019

Abstract
The aim of this study was to examine the actual procedures with food in households and consumer attitudes about food waste. The survey was conducted in 83 households in Belgrade, Serbia. All participants were interviewed using a standardized questionnaire. The results obtained show that awareness of food waste is at a satisfactory level, but the actual situation is that food is discarded in large quantities, even though people are aware of what a global problem this is. Large contradictions were observed among the respondents’ answers in this study. Respondents who stated that they never discard food, in further responses, declared they throw away significant amounts of food for various reasons (too long storage, overconsumption, improper preparation, etc.). We conclude that people are either unaware of how much food they discard, or they do not want to admit it to themselves. However, participants largely have a sense of guilt about discarding food. This indicates consumer awareness of food waste, and is an encouraging sign that further education could be effective in consumers taking into account their food waste habits, starting from procurement planning, through storage and preparation.
Physical properties (pH and aw value) of fermented sausages inoculated with Yersinia enterocolitica
Authors: Mitrovic R R , Jankovic V V , Ciric J S , Djordjevic V Z , Juric Z Lj , Mitrovic-Stanivuk M R , Baltic B M 
Published: October 2019

Abstract
Fermented sausages are produced without heat treatment, and the conservative effect is achieved by a combination of three factors: pH, lowering the water activity (aw) and the creation of lactic acid. Here, we summarize the results of the production of fermented sausages inoculated with Y. enterocolitica, with the added starter culture. Number of Y.enterocolitica declined during ripening, as did pH and aw value. During the ripening process, the aw of fermented sausages of narrower and wider diameters significantly decreased and was lower in sausages in which starter culture was used. At the end of the production process, there were no significant differences between the physical parameters of sausage quality (pH and aw) among fermented sausages with and without added starter culture, and between sausages with narrower and wider diameters.