bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pharmacy services and psychiatric patient satisfaction among community-based health insurance users in Ethiopia
Authors: Chanie Gashaw Sisay , Atalay Wagaye , Alemayehu Tekletsadik Tekleslassie , Wube Bayleyegn Zemenu , Tadesse Gebresilassie , Fentahun Setegn , Wassie Yilkal Abebaw , Tesfaye Tegenu Chanie , Geremew Gebremariam Wulie 
Published: November 2024

BackgroundThe development of community-based health insurance (CBHI) was driven by the need to provide economic protection for the poor against unexpected healthcare expenses. This can lead to increased patient satisfaction with their overall care. Maintaining high levels of client satisfaction with pharmacy services is crucial for effectively treating patients with psychiatric disorders. Therefore, the purpose of this study was to assess the impact of pharmacy services on psychiatry patient satisfaction among users of CBHI in the psychiatric setting.MethodsA multicenter cross-sectional study was conducted at the psychiatric clinics with a systematic random sampling technique between December 2023 and May 2024. A structured interview questionnaire was used to gather data. Both bivariate and multivariate analysis were employed. In multivariate analysis, variables having a p-value of &lt; 0.05 were deemed statistically significant.ResultA total of 420 participants were enrolled with a response rate of 99.5%. Overall, 261 (62.1%) of the patients reported being satisfied with the pharmacy service. However, a significant number of patients expressed dissatisfaction with certain aspects of the service, such as pharmacist counseling on medication side effects (43.1%), medication interactions (36.9%), and labeling and dispensing of medicines (42.4%). According to the study, living in an urban area [AOR = 2.0; 95% CI (1.25, 3.2); P = 0.04], being between the ages of 18–35 and 36–44 [AOR = 2.7, 95% CI (1.38, 5.3), p = 0.04] and [AOR = 3.3, 95% CI (1.6, 5.7), p = 0.001] respectively. First and second visits to the institution [AOR = 2.2; 95% CI (1.15, 4.4); P = 0.01] and [AOR = 1.9; 95% CI (1.13, 3.3); P = 0.01] respectively. Having two psychiatric disorders [AOR = 1.8, 95% CI (1.07, 3.07), p = 0.02] and all drug availability [AOR = 1.5, 95% CI (1.3, 3.43), p = 0.02], were positively associated with psychiatry patient pharmacy service satisfaction.ConclusionIn this study, the users of CBHI psychiatric patients were generally satisfied with the pharmacy service. Additionally, being urban residency age (18–44 years), first and second visits to the institution, having two psychiatric disorders and all drug availability were found to have a significant impact on psychiatric patient pharmacy services satisfaction.
KS-cSCC-1 and KS-cSCC-2: two novel cutaneous squamous cell carcinoma cell lines established from Japanese patients
Authors: Ito Takamichi , Tanaka Yuka , Kaku-Ito Yumiko , Tanegashima Keiko , Imajima Mao , Ichiki Toshio , Nakahara Takeshi 
Published: November 2024

IntroductionCutaneous squamous cell carcinoma (cSCC) is a common form of skin cancer. Less accessibility to the cSCC cell lines has limited analyses of this disease. Thus, we here aimed to establish novel cSCC cell lines from patient's cSCC lesions.MethodsTwo novel cSCC cell lines (named KS-cSCC-1 and KS-cSCC-2) were established from an axillary lymph node metastasis of a Japanese female and an inguinal lymph node metastasis of a Japanese male. The characteristics of the established cell lines were assessed by in vitro analyses.ResultsThe cells were successfully maintained for more than 9 months, with a doubling time of 47.5 ± 1.11 h (KS-cSCC-1) and 39.2 ± 5.78 h (KS-cSCC-2). The cell lines exhibited constant growth, spheroid formation, and invasiveness. Short tandem repeat analyses and immunohistochemistry confirmed that both cell lines are identical to their original tumor. The KS-cSCC-1 cells were weakly positive for CK14 and strongly positive for CK10, while the KS-cSCC-2 showed opposite expression patterns. Chemosensitivity of the cell lines was further tested and the cells were sensitive to anticancer drugs which are used to treat cSCC.ConclusionThe KS-cSCC-1 and KS-cSCC-2 cell lines were promising resources for basic and preclinical research on cSCC to better define the tumor characteristics and treatment strategy of this cancer.
Optimization of diagnosis and treatment of hematological diseases via artificial intelligence
Authors: Wang Shi-Xuan , Huang Zou-Fang , Li Jing , Wu Yin , Du Jun , Li Ting 
Published: November 2024

BackgroundOptimizing the diagnosis and treatment of hematological diseases is a challenging yet crucial research area. Effective treatment plans typically require the comprehensive integration of cell morphology, immunology, cytogenetics, and molecular biology. These plans also consider patient-specific factors such as disease stage, age, and genetic mutation status. With the advancement of artificial intelligence (AI), more “AI + medical” application models are emerging. In clinical practice, many AI-assisted systems have been successfully applied to the diagnosis and treatment of hematological diseases, enhancing precision and efficiency and offering valuable solutions for clinical practice.ObjectiveThis study summarizes the research progress of various AI-assisted systems applied in the clinical diagnosis and treatment of hematological diseases, with a focus on their application in morphology, immunology, cytogenetics, and molecular biology diagnosis, as well as prognosis prediction and treatment.MethodsUsing PubMed, Web of Science, and other network search engines, we conducted a literature search on studies from the past 5 years using the main keywords “artificial intelligence” and “hematological diseases.” We classified the clinical applications of AI systems according to the diagnosis and treatment. We outline and summarize the current advancements in AI for optimizing the diagnosis and treatment of hematological diseases, as well as the difficulties and challenges in promoting the standardization of clinical diagnosis and treatment in this field.ResultsAI can significantly shorten turnaround times, reduce diagnostic costs, and accurately predict disease outcomes through applications in image-recognition technology, genomic data analysis, data mining, pattern recognition, and personalized medicine. However, several challenges remain, including the lack of AI product standards, standardized data, medical–industrial collaboration, and the complexity and non-interpretability of AI systems. In addition, regulatory gaps can lead to data privacy issues. Therefore, more research and improvements are needed to fully leverage the potential of AI to promote standardization of the clinical diagnosis and treatment of hematological diseases.ConclusionOur results serve as a reference point for the clinical diagnosis and treatment of hematological diseases and the development of AI-assisted clinical diagnosis and treatment systems. We offer suggestions for further development of AI in hematology and standardization of clinical diagnosis and treatment.
Impact of a virtual antenatal intervention for improved diet and iron intake in Kapilvastu district, Nepal - the VALID randomized controlled trial
Authors: Saville Naomi M. , Bhattarai Sanju , Giri Santosh , Sapkota Suprich , Morrison Joanna , Thapaliya Bibhu , Bhattarai Basudev , Yadav Samata , Arjyal Abriti , Copas Andrew , Haghparast-Bidgoli Hassan , Harris-Fry Helen , Piya Reecha , Baral Sushil C. , Hillman Sara L. 
Published: November 2024

IntroductionCounseling, together with iron and folic acid supplements, can improve hemoglobin levels in pregnant women, but few interventions have tested a virtual method of delivering counseling. We hypothesized that a virtual counseling intervention delivered via a mobile device (mHealth) would prevent and treat anemia, compared with routine antenatal care (ANC).MethodsVirtual antenatal intervention for improved diet and iron intake (VALID) was a non-blinded parallel group two-arm, individually randomized superiority trial (1:1 allocation). Participants were pregnant women who were married, aged 13–49 years, able to answer questions, 12–28 weeks’ gestation and living in Kapilvastu district, Nepal. Women were randomized to receive routine ANC (control arm), or ANC plus a virtual antenatal intervention of two problem-solving counseling sessions via video call. The primary outcome was iron folic acid (IFA) tablet compliance (consumption on 12 or more days of the previous 14 days). Secondary outcomes were dietary diversity, promoted food consumption, iron bioavailability enhancement, and knowledge of iron-rich foods. Primary logistic regression analysis was by intention-to-treat, adjusting for baseline values.ResultsWe enrolled 319 pregnant women (161 control, 158 intervention) from 23 January 2022 to 6 May 2022 and analyzed outcomes in 144 control and 127 intervention women. Compliance with IFA increased in both arms. In the intervention arm, compliance increased by 29.7 percentage points (pp) (49.0–78.7%) and 19.8 pp. in the control arm (53.8–73.6%). Despite the more significant increase in the intervention arm, we found no intervention effect upon IFA compliance (adjusted odds ratio [aOR] 1.33; 95% confidence interval [CI]: 0.75, 2.35; p = 0.334), dietary diversity, or ANC visits. The intervention increased knowledge of iron-rich foods (coefficient 0.96; 95% CI: 0.50, 1.41; p &lt; 0.001), consumption of promoted foods (aOR: 1.81; 95% CI: 1.08, 3.02; p = 0.023), behavior to enhance iron bioavailability (aOR: 4.41; 95% CI: 1.23, 15.83; p = 0.023), and coronavirus disease 2019 (COVID-19) knowledge (aOR: 4.06; 95% CI: 1.56, 10.54; p = 0.004). The total intervention cost was US$35,193, and the cost per pregnant woman receiving two virtual counseling sessions was US$277.ConclusionVirtual counseling can improve antenatal health behaviors, such as the consumption of promoted foods and methods to enhance bioavailability. Improved IFA consumption and ANC attendance may require additional family/community support.Clinical trial registrationhttps://www.isrctn.com/ISRCTN17842200, identifier ISRCTN17842200.
Protecting optimal childhood growth: systematic nutritional screening, assessment, and intervention for children at risk of malnutrition in the Kingdom of Saudi Arabia
Authors: Murray Robert D. , Shaaban Sanaa Y. , Al Amrani Mohammed , Aldekhail Wajeeh , Alhaffaf Faisal A. , Alharbi Abdulaziz O. , Almehaidib Ali , Al-Suyufi Yasir , Al-Turaiki Muath , Amin Ahmed , Hasosah Mohammed Y. , Alkhormi Musa , Mirza Ziyad T. , Sleiman Rola , Sukkar Ghassan 
Published: November 2024

BackgroundIn 2024, the Kingdom of Saudi Arabia Advisory Board on Pediatric Nutrition (KSA-ABPN) reviewed childhood undernutrition in the Middle East. We sought to foster efficient nutritional care for infants and children at nutritional risk. Severe malnutrition due to starvation is rare in Saudi Arabia, so we focused on early recognition and treatment of children with mild growth impairment that forewarns risk for further nutritional decline. This paper summarizes our findings and introduces a recommended guide for nutritional screening, assessment, and follow-up interventions.ObjectiveThe KSA-ABPN aimed to build an algorithm with pathways and tools to facilitate up-to-date nutrition-care practices for infants and children. The algorithm is intended to encourage consistent professional training-for and use-of validated tools, adoption of standardized thresholds for intervention, and delivery of nutritional support. Consistent care will increase opportunities for comparative analyses of various treatment strategies and their health and cost outcomes.RecommendationsWe developed a 4-stage algorithm for identifying and caring for children at nutritional risk: (i) screening for clinical risk factors and age-related growth measures, (ii) observation of malnutrition-related physical signs, diet history, and/or laboratory detection of evidence indicating specific nutrient deficiencies, (iii) assessment of the severity of nutritional deficit, and (iv) development of a patient-specific Nutrition Care Plan that includes diet counseling, supplementation, routine monitoring, and follow-up.ConclusionsBy helping professionals identify nutritional risk and specific nutritional deficits in infants and children early in the clinical course, we seek to expand quality nutritional care and ensure that children grow and develop fully.
Characterization of sarcoma topography in Li-Fraumeni syndrome
Authors: Brockman Karin J. , Thompson Mone’t B. , Mirabello Lisa , Savage Sharon A. , Malayeri Ashkan , Hatton Jessica N. , Khincha Payal P. 
Published: November 2024

IntroductionLi-Fraumeni syndrome (LFS) is a hereditary cancer predisposition syndrome primarily caused by germline TP53 pathogenic/likely pathogenic (P/LP) variants. Soft tissue and bone sarcomas are among the most frequently occurring of the many LFS-associated cancer types. Cancer screening recommendations for LFS are centered around annual whole-body MRI (wbMRI), the interpretation of which can be challenging. This study aims to characterize sarcoma topography in LFS.MethodsStudy subjects included individuals from clinically and genetically ascertained cohorts of germline TP53 variant-carriers, namely the National Cancer Institute’s LFS longitudinal cohort study (NCI-LFS), the NCI Genetic Epidemiology of Osteosarcoma (NCI-GEO) study, and the germline TP53 Database.ResultsData was aggregated for a total of 160 sarcomas that had detailed topography available. Abdominal sarcomas and extremity osteosarcomas were among the most frequent locations of sarcomas. Chi-squared analyses showed no statistical differences in sarcoma topography based on age (pediatric vs adult) or sex (male vs female). A case series of sarcomas from the NCI-LFS study highlights the diagnostic challenges due to topography-related imaging. DiscussionWhile LFS-related sarcomas frequently occur in expected locations such as the extremities, they also occur in less typical sites, leading to difficulties in discerning between differential diagnoses on wbMRI and imaging. Prospective collection of detailed cancer topography in individuals with LFS will further aid in recommendations for radiologic interpretation and personalized screening in individuals with LFS.
Transcriptome and metabolome analyses provide crucial insights into the adaptation of chieh-qua to Fusarium oxysporum infection
Authors: Qiao Yanchun , Peng Jiazhu , Wu Bei , Wang Min , He Guoping , Peng Qingwu , Gao Yin , Liu Yuping , Yang Songguang , Dai Xiuchun 
Published: November 2024

IntroductionChieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) is a wax gourd variety that is generally susceptible to infection and damage by Fusarium oxysporum during its cultivation. Therefore, analyzing the adaption mechanism of chieh-qua to F. Oxysporum infection is of great significance for cultivating resistant varieties.MethodsThrough comparative transcriptome analysis, comparative metabolome analysis, integrated analysis of transcriptome and metabolome and between F. Oxysporum infected samples and control samples of susceptible linesResultsThis study found that proteins such as NPR1, TGA and PR1 in plant hormone signal transduction pathway were up-regulated after infection, which may activate a series of plant secondary metabolic synthesis pathways. In addition, the expression of 27 genes in the flavonoid biosynthetic process in resistant lines after infection was significantly higher than that in susceptible lines, indicating that these genes may be involved in fungal resistance. This study also found that alternative splicing of genes may play an important role in responding to F. Oxysporum infection. For example, plant protein kinase genes such as EDR1, SRK2E and KIPK1 were not differentially expressed after F. Oxysporum infection, but the transcripts they produced differ at the transcription level. Finally, through comparative metabolome analysis, this study identified potentially functional substances such as oxalic acid that increased in content after F. Oxysporum infection. Through integrated analysis of transcriptome and metabolome, some differential expressed genes significantly related to differential metabolites were also identified.DiscussionThis study provides a basis for understanding and utilizing chieh-qua’s infection mechanism of F. Oxysporum through analysis of the transcriptome and metabolome.
Intervention with institutional caregivers for the purpose of supportive education for disadvantaged children: 3C
Authors: Dere Zeynep , Akagündüz Eğrikılınç Duygu 
Published: November 2024

IntroductionChildren in institutional care may have limited access to educational opportunities compared to children in parental care. Improving and enriching the educational practices of these children is important for their well-being. In this study, the Child Development, Communication and Care (3C) Training Program was prepared by the researchers. The 3C education approach emphasizes early childhood development and education. Particular focus is on the use of communication techniques in the education of young children. Our goal with 3C is to increase caregivers’ knowledge of child development and support them to communicate effectively with children. Through this curriculum framework, children are encouraged to interact effectively and empathically with others. Therefore, the curriculum framework emphasizes understanding emotions and building trusting relationships as key components in the holistic development of young disadvantaged children.MethodsA quasi-experimental design with pre-test-post-control group was used in the study. 16 sessions of 3C were applied to the personnel in the experimental group via distance education. Data were collected from the participants before and after the start of the training program by using the Child Education Competence Scale-Parent Form, Personal Information Form, and Training Evaluation Questionnaire.ResultsAs a result, significant differences were obtained between the experimental and control groups in favor of the group receiving training (z = 3.57, p&lt;0.05). Accordingly, it can be said that 3C contributes to the holistic development of the child.DiscussionIt is hoped that such work could enhance the developmental outcomes of young children in institutional care and improve the quality of early childhood education.
Physiological parameters to support attention deficit hyperactivity disorder diagnosis in children: a multiparametric approach
Authors: Castro Ribeiro Thais , García Pagès Esther , Huguet Anna , Alda Jose A. , Badiella Llorenç , Aguiló Jordi 
Published: November 2024

IntroductionAttention deficit hyperactivity disorder (ADHD) is a high-prevalent neurodevelopmental disorder characterized by inattention, impulsivity, and hyperactivity, frequently co-occurring with other psychiatric and medical conditions. Current diagnosis is time-consuming and often delays effective treatment; to date, no valid biomarker has been identified to facilitate this process. Research has linked the core symptoms of ADHD to autonomic dysfunction resulting from impaired arousal modulation, which contributes to physiological abnormalities that may serve as useful biomarkers for the disorder. While recent research has explored alternative objective assessment tools, few have specifically focused on studying ADHD autonomic dysregulation through physiological parameters. This study aimed to design a multiparametric physiological model to support ADHD diagnosis.MethodsIn this observational study we non-invasively analyzed heart rate variability (HRV), electrodermal activity (EDA), respiration, and skin temperature parameters of 69 treatment-naïve ADHD children and 29 typically developing (TD) controls (7-12 years old). To identify the most relevant parameters to discriminate ADHD children from controls, we explored the physiological behavior at baseline and during a sustained attention task and applied a logistic regression procedure.ResultsADHD children showed increased HRV and lower EDA at baseline. The stress-inducing task elicits higher reactivity for EDA, pulse arrival time (PAT), and respiratory frequency in the ADHD group. The final classification model included 4 physiological parameters and was adjusted by gender and age. A good capacity to discriminate between ADHD children and TD controls was obtained, with an accuracy rate of 85.5% and an AUC of 0.95.DiscussionOur findings suggest that a multiparametric physiological model constitutes an accurate tool that can be easily employed to support ADHD diagnosis in clinical practice. The discrimination capacity of the model may be analyzed in larger samples to confirm the possibility of generalization.
Acceptability of identification and management of perinatal anxiety: a qualitative interview study with postnatal women
Authors: Meades Rose , Moran Patricia M. , Hutton Una , Khan Rafiyah , Maxwell Margaret , Cheyne Helen , Delicate Amy , Shakespeare Judy , Hollins Kathryn , Pisavadia Kalpa , Doungsong Kodchawan (Pim) , Edwards Rhiannon Tudor , Sinesi Andrea , Ayers Susan 
Published: November 2024

BackgroundAnxiety in pregnancy and postpartum is highly prevalent but under-recognized and few women receive adequate support or treatment. Identification and management of perinatal anxiety must be acceptable to women in the perinatal period to ensure that women receive appropriate care when needed. We aimed to understand the acceptability to women of how anxiety was identified and managed by healthcare professionals.MethodWe conducted in-depth qualitative interviews with 60 women across England and Scotland approximately 10 months after birth. Women were sampled from an existing systematically recruited cohort of 2,243 women who recorded mental health throughout pregnancy and after birth. All women met criteria for further assessment of their mental health by a healthcare professional. We analyzed the data using a theoretical framework of acceptability of healthcare interventions.ResultsInterview data fitted the seven constructs within the theoretical framework of acceptability. Women valued support before professional treatment but were poorly informed about available services. Services which treated women as individuals, which were accessible and in which there was continuity of healthcare professional were endorsed. Experience of poor maternity services increased anxiety and seeing multiple midwives dissuaded women from engaging in conversations about mental health. Having a trusted relationship with a healthcare professional facilitated conversation about and disclosure of mental health problems.ConclusionWomen’s experiences would be improved if given the opportunity to form a trusting relationship with a healthcare provider. Interventions offering support before professional treatment may be valued and suitable for some women. Clear information about support services and treatment options available for perinatal mental health problems should be given. Physiological aspects of maternity care impacts women’s mental health and trust in services needs to be restored. Findings can be used to inform clinical guidelines and research on acceptable perinatal care pathways in pregnancy and after birth and future research.