bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptional profiling of macrophages infected with Fonsecaea monophora
Authors: Shi Minglan , Sun Jiufeng , Lu Sha , Qin Jinglin , Xi Liyan , Zhang Junmin 
Published: January 2019

SummaryFonsecaea monophora is a member of dematiaceous fungi capable of causing chromoblastomycosis through traumatic injury. However, little is known about the pathogenesis and early interactions between F. monophora and host. The aim of this study was to explore the potential mechanism of macrophages against F. monophora, especially the role of melanin during the pathogenic process. We carried out RNA sequencing based on the Illumina system. It showed that according to melanin contents, different strains of F. monophora induced different transcriptional profilings in macrophages. Functional analyses suggested the biological functions of differentially expressed genes were closely related to immune response, and the melanin might affect the interactions by regulating the MAPK signalling pathway of macrophages. Our results provide insights into the pathogenesis of infection by F. monophora conidia.
Roles of protective factors and risk factors in suicidal ideation among adolescents in Taiwan
Authors: Huang Hui‐Wen , Wang Ruey‐Hsia 
Published: January 2019

AbstractObjectiveTo examine the role of protective factors and risk factors in suicidal ideation among adolescents in Taiwan based on a resilience protective model.Design and SampleA cross‐sectional design was employed. A total of 390 adolescents aged 15–19 years were recruited from four high schools in Taiwan by stratified random sampling.MethodsAn anonymous self‐report questionnaire was used to collect demographic characteristics, suicidal ideation, depressive symptoms, life stress, emotion‐focused coping, self‐esteem, and problem‐focused coping. Hierarchical multiple regression was used to test the hypotheses.ResultsDepressive symptoms were significantly and positively correlated with suicidal ideation. The interaction between depressive symptoms and self‐esteem as well as between emotion‐focused coping and problem‐focused coping were significantly and negatively correlated with suicidal ideation.ConclusionsDepressive symptoms were a risk factor of suicidal ideation. Self‐esteem can moderate the negative effect of depressive symptoms on suicidal ideation. Problem‐focused coping can moderate the negative effects of emotion‐focused coping on suicidal ideation. Public health nurses could cooperate with school nurses to periodically screen depressive symptoms and provide early interventions. Teaching parents and teachers methods for improving self‐esteem of adolescents and enhancing adolescents to apply problem‐focused coping strategies could be useful to reduce suicidal ideation of adolescents.
Syllables constitute proximate units for Mandarin speakers: Electrophysiological evidence from a masked priming task
Authors: Zhang Qingfang , Damian Markus F. 
Published: January 2019

AbstractLanguages may differ regarding the primary mental unit of phonological encoding in spoken production, with models of speakers of Indo‐European languages generally assuming a central role for phonemes, but spoken Chinese production potentially attributing a more prominent role to syllables. In the present study, native Mandarin Chinese speakers named objects that were preceded by briefly presented and masked prime words, which were form related and either matched or mismatched concerning their syllabic structure, or were unrelated. Behavioral results showed a previously reported interaction between prime and target syllable type. Concurrently recorded EEG also exhibited this interaction and further revealed that syllable overlap modulated ERPs mainly in the time window of 300–400 ms after picture onset. By contrast, phonemic overlap modulated ERPs from 500 ms to 600 ms. This pattern might suggest that speakers retrieved syllables before phonemes and strengthens the claim that for Chinese individuals syllables constitute primary functional representations (“proximate units”).
Age‐dependent hypoxia‐induced PD‐L1 upregulation in patients with obstructive sleep apnoea
Authors: Cubillos‐Zapata Carolina , Balbás‐García Cristina , Avendaño‐Ortiz Jose , Toledano Victor , Torres Marta , Almendros Isaac , Casitas Raquel , Zamarrón Ester , García‐Sánchez Aldara , Feliu Jaime , Aguirre Luis A. , Farre Ramón , López‐Collazo Eduardo , García‐Rio Francisco 
Published: January 2019

ABSTRACTBackground and objectiveIn obstructive sleep apnoea (OSA), intermittent hypoxia (IH) compromises immune surveillance through the upregulation of the programmed cell death‐1 (PD‐1) receptor and its ligand (PD‐L1). Because the risk of OSA‐related cancer depends on age, we assessed PD‐L1/PD‐1 expression in middle‐aged and older patients with OSA as well as in a murine model.MethodsPD‐L1 expression was studied in 41 patients with severe OSA and 40 healthy volunteers (HV), divided into two groups (≤55 and >55 years of age). We used flow cytometry, quantitative PCR (qPCR) and ELISA to determine PD‐L1 expression on monocytes and plasma PD‐L1 protein levels. Moreover, we analysed PD‐L1 expression on an in vivo IH model with old and young mice.ResultsIn subjects up to 55 years of age, severe OSA increased PD‐L1 surface protein and mRNA level expression on monocytes and soluble‐PD‐L1 protein concentration in plasma compared to HV. PD‐L1 and hypoxia‐induced factor (HIF)‐1α expression correlated with age in HV, whereas in patients with OSA there was a negative relationship. In the mice exposed to IH, PD‐L1 expression on F4/80+ splenocytes was also only increased in young animals. HIF‐1α expression was significantly higher in patients with OSA than in HV in subjects up to 55 years of age, while PD‐L1 expression in monocytes was related to HIF‐1α expression in young patients with OSA.ConclusionPD‐L1 upregulation in patients with OSA as a consequence of HIF‐1α activation occurs mainly in young patients. In older patients with OSA, upregulation was not detected, possibly due to impaired oxygen sensitivity.
Noninvasive analysis of skin proteins in healthy Chinese subjects using an Orbitrap Fusion Tribrid mass spectrometer
Authors: Ma Jie , Xu Shuangjun , Wang Xiaomeng , Zhang Jing , Wang Yaochi , Liu Mengting , Jin Ling , Wu Mingshun , Qian Danfeng , Li Xueying , Zhen Qi , Guo Huimin , Gao Jinping , Yang Sen , Zhang Xuejun 
Published: January 2019

AbstractObjectiveThe objective of this study was to perform noninvasive analysis of skin proteins in a healthy Chinese population using label‐free nanoflow liquid chromatography‐mass spectrometry (nLC‐MS).Materials and MethodsFive consecutive tape strippings were obtained from the volar forearm skin of healthy Chinese subjects. Proteins were extracted, and trypsin‐digested peptides were analyzed by a nanochromatography instrument coupled to an Orbitrap Fusion Tribrid mass spectrometer. Data‐dependent acquisition allowed protein identification, which was performed by using Proteome Discoverer software (v2.2).ResultsIn this study, we identified 80 common proteins that were expressed in the skin of healthy Chinese volunteers and divided these proteins into 16 categories, including keratins, cornified envelope proteins, and enzymes associated with substance metabolism. These proteins were closely associated with multiple functions of the skin barrier.ConclusionThis study provides a noninvasive method to analyze healthy human epidermal proteins, which are closely associated with the skin barrier. In addition, this study provides a reference for further studies on the application of proteomic technologies to investigate the role of human epidermal proteins in health and disease.
Three‐dimensional reconstruction of skin disease using multi‐view mobile images
Authors: Hong Geeyun , Lee Onseok 
Published: January 2019

AbstractBackground/PurposeIn skin beauty area, interest in diagnosis and management methods for acne is increasing. However, it is difficult to diagnose protruding skin disease by two‐dimensional (2D) image. Three‐dimensional (3D) approach is needed. The purpose of this study was to propose a system for reconstructing 3D model (visual hull) of 2D images obtained using mobile devices.MethodsAcne images were acquired using a 3D printing mobile image acquisition system. Using two attached mirrors, five multi‐view acne silhouette images were obtained. They were then, reconstructed to 3D. To measure the volume of the formed 3D model (visual hull), post‐processing, calculation, and texture mapping were performed. The volume of acne samples designed with 3D printing was compared with the volume calculated in the produced system using statistical analysis program.ResultsThe 3D modeling program demonstrated its superiority by showing high correlation (r = 0.783) between the actual volume of acne and the volume calculated by the system. The 3D model (visual hull) was successfully reconstructed by capturing 2D images of actual acne.ConclusionIn this study, we created a system to reconstruct small sized protruding skin disease images such as acne obtained with mobile devices. The 3D printing system was used to obtain images from mobile camera and reconstructed to 3D. To verify the volume of the reconstructed model, 3D printing samples were produced and compared with the calculated actual volume. It can be used, for initial diagnosis by receiving information about the protruding skin disease without space‐time constraints using a mobile device.
Reviewer Acknowledgements
Authors: Smith Robert 
Published: January 2019

Journal of Education and Training Studies (JETS) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether JETS publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue.Reviewers for Volume 6, Number 12aAdalberto Felipe Martinez, Federal University of São Carlos, BrazilBahman Aalizadeh, IranEnisa Mede, Bahcesehir University, TurkeyLorna T. Enerva, Polytechnic University of the Philippines, PhilippinesMassimiliano Barattucci, Ecampus University, ItalyMeral Seker, Alanya Alaaddin Keykubat University, TurkeyMichael Wall, Independent Researcher in Music and Music Education, USANiveen M. Zayed, MENA College of Management, JordanShabnam AsadKarami, IranSima Mokari Saei, IranVjacheslav Ivanovich Babich, Luhansk Taras Shevchenko National University, Ukraine Robert SmithEditorial AssistantOn behalf of,The Editorial Board of Journal of Education and Training StudiesRedfame Publishing9450 SW Gemini Dr. #99416Beaverton, OR 97008, USAURL: http://jets.redfame.com
Parameters affecting postoperative success of surgery for stage 4A/4B ROP
Authors: Özsaygili Cemal , Ozdek Sengul , Ozmen Mehmet Cuneyt , Atalay Hatice Tuba , Yalinbas Yeter Duygu 
Published: January 2019

PurposeTo describe the long-term anatomical and functional results of surgery for retinal detachment (RD) associated with stage 4 retinopathy of prematurity (ROP) and patient and surgery-related factors affecting postoperative success.DesignRetrospective case series at a single tertiary referral paediatric vitreoretinal practice.MethodsOne hundred and twenty-one eyes of 82 infants (40 female/42 male) who underwent lens-sparing vitrectomy (LSV) or lensectomy with vitrectomy surgery for stage 4A and 4B ROP at Gazi University Department of Ophthalmology between 2011 and 2016 were enrolled in this study. Patient characteristics including gestational age, birth weight, gender, stage of ROP at presentation, preoperative treatment (laser, anti-vascular endothelial growth factor (VEGF) or combined), anatomical and functional outcome and complications were recorded. The effect of birth weight, gestational age, presence of plus disease, preoperative treatment status, surgically induced posterior hyaloid detachment, postoperative vitreous haemorrhage and iatrogenic retinal tear formation on anatomical and functional results was evaluated.Results61.2% of the eyes were stage 4A and 38.8% were stage 4B ROP. The mean follow-up was 24.5 months. 18.2% of the eyes had no preoperative treatment. Anatomical success was 86.5% for stage 4A and 68.1% for stage 4B at the first year, 91.7% for stage 4A and 69.4% for stage 4B at the second year, and 95.8% for stage 4A and 57.9% for stage 4B at the third year. Functional success was 85.1% for stage 4A and 65.9% for stage 4B at the first year, 89.6% for stage 4A and 61.1% for stage 4B at the second year, and 87.5% for stage 4A and 57.8% for stage 4B at the third year. The mean visual acuity was 1.12±0.34 logarithm of the minimum angle of resolution (logMAR) for stage 4A and 1.34±0.32 logMAR at the 3-year follow-up duration (p>0.05). There was preoperative plus disease in 59.5% of the eyes. Subsequent retinal surgeries were required in 17.4% of the eyes. Presence of plus disease and absence of preoperative treatment, iatrogenic retinal tear formation and postoperative vitreous haemorrhage were found to have significant negative effects, while surgical induction of posterior hyaloid detachment and sparing the lens intraoperatively affected the anatomical and functional results positively.ConclusionsSurgery for stage 4 ROP-associated RD resulted in encouraging anatomical and functional outcomes and the results are even better in eyes with preoperative (laser/anti-VEGF) treatment, LSV and surgically induced posterior hyaloid detachment.
Acceptability and feasibility pilot randomised controlled trial of medical skin camouflage for recovery of women prisoners with self-harm scarring (COVER): the study protocol
Authors: Mitchell Heather , Abel Kathryn M , Dunlop Brendan James , Walker Tammi , Ranote Sandeep , Robinson Louise , Edgar Fiona , Millington Tracy , Meacock Rachel , Shaw Jenny , Gutridge Kerry 
Published: January 2019

IntroductionSelf-harm in prison is a major public health concern. Less than 5% of UK prisoners are women, but they carry out more than a fifth of prison self-harm. Scars resulting from self-harm can be traumatising and stigmatising, yet there has been little focus on recovery of women prisoners with self-harm scarring. Medical skin camouflage (MSC) clinics treat individuals with disfiguring skin conditions, with evidence of improved well-being, self-esteem and social interactions. Only one community study has piloted the use of MSC for self-harm scarring.Methods and analysisWe describe an acceptability and feasibility pilot randomised controlled trial; the first to examine MSC for women prisoners who self-harm. We aim to randomise 20–25 women prisoners to a 6-week MSC intervention and 20–25 to a waitlist control (to receive the MSC after the study period). We aim to train at least 6–10 long-term prisoners with personal experience of self-harm to deliver the intervention. Before and after intervention, we will pilot collection of women-centred outcomes, including quality of life, well-being and self-esteem. We will pilot collection of self-harm incidents during the intervention, resources used to manage/treat self-harm and follow-up of women at 12 weeks from baseline. Data on recruitment, retention and dropout will be recorded. We aim for the acceptability of the intervention to prison staff and women prisoners to be explored in qualitative interviews and focus groups.Ethics and disseminationEthical approval for COVER has been granted by the North East–York Research Ethics Committee (REC) for phases 1 and 2 (reference: 16/NE/0030) and West of Scotland REC 3 for phases 3 and 4 (reference: 16/WS/0155). Informed consent will be the primary consideration; it will be made clear that participation will have no effect on life in prison or eligibility for parole. Due to the nature of the study, disclosures of serious self-harm may need to be reported to prison officials. We aim for findings to be disseminated via events at the study prison, presentations at national/international conferences, journal publications, prison governor meetings and university/National Health Service trust communications.Trial registration numberNCT02638974; Pre-results.
Assessment of public oral healthcare services in Curitiba, Brazil: a cross-sectional study using the Primary Care Assessment Tool (PCATool)
Authors: Nascimento Antonio Carlos , Moysés Simone Tetü , Werneck Renata Iani , Gabardo Marilisa Carneiro Leão , Moysés Samuel Jorge 
Published: January 2019

ObjectivesTo compare two different models of public oral health in primary care services, a so-called family health strategy (FHS), as opposed to non-FHS services designated as ‘conventional’ healthcare (CHC), regarding the presence and extent of the attributes of ‘good’ primary healthcare (PHC). The null hypothesis of this study is that the attributes do not differ between the FHS and CHC.DesignCross-sectional.SettingPublic PHC services in Curitiba, the state capital of Paraná.ParticipantsPHC users of the public oral health network (n=900) and dentists active in this municipal network (n=203).Primary and secondary outcome measuresThe Primary Care Assessment Tool (PCATool)-Dentists and PCATool-Users were used to analyse the primary outcomes (‘essential’ attributes) and secondary outcomes (‘derived’ attributes) in the PHC.ResultsOverall, the primary care services in oral health were well evaluated, both by users and by dentists, with mean scores ascribed to PHC attributes mostly above the cut-off point (6.6). The exception for users were affiliation (6.36; 95% CI 6.11 to 6.60) and accessibility (5.83; 95% CI 5.78 to 5.89); and for dentists the accessibility (5.80; 95% CI 5.63 to 5.96). When comparing FHS and CHC, there was a superiority of the FHS model, which reached a general mean score of 7.53 (95% CI 7.48 to 7.58) among users and 7.56 (95% CI 7.45 to 7.67) among dentists; on the other hand, the CHC general mean score was of 6.61 (95% CI 6.49 to 6.73) and 6.68 (95% CI 6.56 to 6.80) respectively for users and dentists.ConclusionsThe results reveal a reasonable level of attainment of PHC attributes in the services investigated. Nevertheless, public health managers should make efforts to reduce the difficulties faced by users in accessing dental care. The more positive results achieved by FHS services indicate that the provision of oral healthcare under this strategy should be expanded.
Association of lifestyle modification and pharmacological adherence on blood pressure control among patients with hypertension at Kenyatta National Hospital, Kenya: a cross-sectional study
Authors: Kimani Samuel , Mirie Waithira , Chege Margaret , Okube Okubatsion Tekeste , Muniu Samuel 
Published: January 2019

ObjectiveAssociation of lifestyle modification and pharmacological adherence among patients with hypertension attending a national referral hospital in Kenya.DesignDescriptive, cross-sectional.SettingMedical wards and outpatient clinic of a national referral hospital.ParticipantsPatients (n=229) diagnosed with primary hypertension for at least 6 months.Primary outcomesClinical makers, cholesterol levels, anthropometrics, lifestyle/dietary habits adjusted for age, gender and education; antihypertensive adherence; views on prevention of hypertension and adequacy of hypertension information.ResultsAgeing was associated with elevated diastolic blood pressure (BP) (p<0.05), heart rate (HR) and cholesterol. Females had higher body mass index (BMI). More males reported drinking alcohol and smoking (p<0.001), especially the highly educated. Higher BPs were observed in smokers and drinkers (p<0.05). Daily vegetables and fruits intake were linked to lower BP, HR and BMI (p<0.05). Intake of foods high in saturated fat and cholesterol were associated with raised HR (p<0.05). Respondents on antihypertensive medication, those engaged in healthy lifestyle and took their prescribed medications had lower mean BPs than those on medication only (138/85 vs 140/90). Few respondents (30.8%) considered hypertension as preventable, mainly the single and highly educated (p<0.05). Respondents (53.6%) believed they should stop taking their antihypertensive medication once hypertension is controlled.ConclusionMissed targets for BP control and hypertension-related risks are associated with ageing, female gender, fast food and animal fat intake. Alcohol and smoking is common in males associated with poor BP control. Daily vegetables and fruits intake are associated with better BP control and overall hypertension risk reduction. Observed suboptimal BP control despite pharmacological adherence suggests lifestyle modification is needed besides antihypertensive medication. Interventions should address modifiable risk factors aggravated by age and adverse lifestyles through adopting combined lifestyle modification, pharmacological adherence and tailored expert delivered hypertension-related information.
Combination of the National Early Warning Score (NEWS) and inflammatory biomarkers for early risk stratification in emergency department patients: results of a multinational, observational study
Authors: Eckart Andreas , Hauser Stephanie Isabelle , Kutz Alexander , Haubitz Sebastian , Hausfater Pierre , Amin Devendra , Amin Adina , Huber Andreas , Mueller Beat , Schuetz Philipp 
Published: January 2019

ObjectivesThe National Early Warning Score (NEWS) helps to estimate mortality risk in emergency department (ED) patients. This study aimed to investigate whether the prognostic value of the NEWS at ED admission could be further improved by adding inflammatory blood markers (ie, white cell count (WCC), procalcitonin (PCT) and midregional-proadrenomedullin (MR-proADM).DesignSecondary analysis of a multinational, observational study (TRIAGE study, March 2013–October 2014).SettingThree tertiary care centres in France, Switzerland and the USA.ParticipantsA total of 1303 adult medical patients with complete NEWS data seeking ED care were included in the final analysis. NEWS was calculated retrospectively based on admission data.Main outcome measuresThe primary outcome was all-cause 30-day mortality. Secondary outcome was intensive care unit (ICU) admission. We used multivariate regression analyses to investigate associations of NEWS and blood markers with outcomes and area under the receiver operating curve (AUC) as a measure of discrimination.ResultsOf the 1303 included patients, 54 (4.1%) died within 30 days. The NEWS alone showed fair prognostic accuracy for all-cause 30-day mortality (AUC 0.73), with a multivariate adjusted OR of 1.26 (95% CI 1.13 to 1.40, p<0.001). The AUCs for the prediction of mortality using the inflammatory markers WCC, PCT and MR-proADM were 0.64, 0.71 and 0.78, respectively. Combining NEWS with all three blood markers or only with MR-proADM clearly improved discrimination with an AUC of 0.82 (p=0.002). Combining the three inflammatory markers with NEWS improved prediction of ICU admission (AUC 0.70vs0.65 when using NEWS alone, p=0.006).ConclusionNEWS is helpful in risk stratification of ED patients and can be further improved by the addition of inflammatory blood markers. Future studies should investigate whether risk stratification by NEWS in addition to biomarkers improve site-of-care decision in this patient population.Trial registration numberNCT01768494; Post-results.