bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering the Influence of Meridional versus Facial Isomers in Spin Crossover Complexes
Authors: Lathion Timothée , Guénée Laure , Besnard Céline , Bousseksou Azzedine , Piguet Claude 
Published: September 2018

AbstractChelate coordination of non‐symmetrical didentate pyrazine‐benzimidazole (L1) or pyridine‐benzimidazole (L2) N‐donor ligands around divalent iron in acetonitrile produces stable homoleptic triple‐helical spin crossover [Fe(Lk)3]2+ complexes existing as mixtures of meridional (C1‐symmetry) and facial (C3‐symmetry) isomers in slow exchange on the NMR timescale. The speciation deviates from the expected statistical ratio mer/fac=3:1, a trend assigned to the thermodynamic trans‐influence, combined with solvation effects. Consequently, the observed spin state FeIIlow‐spin↔FeIIhigh‐spin equilibria occurring in [Fe(Lk)3]2+ refer to mixtures of complexes in solution, an issue usually not considered in this field, but which limits rational structure‐properties correlations. Taking advantage of the selective and quantitative formation of isostructural facial isomers in non‐constrained related spin crossover d‐f helicates (HHH)‐[LnFe(Lk)3]5+ (Ln is a trivalent lanthanide, Lk=L5, L6), we propose a novel strategy for assigning pertinent thermodynamic driving forces to each spin crossover triple‐helical isomer. The different enthalpic contributions to the spin state equilibrium found in mer‐[Fe(Lk)3]2+ and fac‐[Fe(Lk)3]2+ reflect the Fe−N bond strengths dictated by the trans‐influence, whereas a concomitant solvent‐based entropic contribution reinforces the latter effect and results in systematic shifts of the spin crossover transitions toward higher temperature in the facial isomers.
Prognostic value of autonomous and controlled motivation in outpatient eating‐disorder treatment
Authors: Sansfaçon Jeanne , Gauvin Lise , Fletcher Émilie , Cottier Danaëlle , Rossi Erika , Kahan Esther , Israël Mimi , Steiger Howard 
Published: September 2018

AbstractAccording to Self‐Determination Theory, when motivation to reach an objective is fully internal, it is said to be “autonomous”; when driven by external incentives, it is said to be “controlled”. Previous research has indicated that autonomously motivated individuals show better response to treatments for eating disorders.ObjectiveIn individuals undergoing different intensities of outpatient treatment for an eating disorder, we sought to assess associations between autonomous and controlled motivations and response to treatment on the one hand, and likelihood of dropping out of treatment, on the other.MethodSeven hundred seventy adults meeting DSM‐5 criteria for an eating disorder (216 with Anorexia Nervosa, 282 with Bulimia Nervosa, and 272 with Other Specified Feeding or Eating Disorder) were included in this study. Before an interval of outpatient treatment, individuals completed the Eating Disorder Examination Questionnaire and the Autonomous and Controlled Motivations for Treatment Questionnaire. Participants completed the Eating Disorder Examination Questionnaire again at one or two subsequent timepoints.ResultsAfter controlling for diagnosis, treatment intensity, and number of previous treatments, analyses showed that higher autonomous motivation was associated with better response on eating‐disorder overall symptoms and lower likelihood of dropping out of treatment. In contrast, controlled motivation was not associated with response to treatment.DiscussionOur results suggest that autonomous motivation has trans‐diagnostic influence upon response to various intensities of treatment for an eating disorder. In support of an autonomy supportive approach to treatment, findings link autonomous motivation with more favorable outcome.
The outcome and characteristics of patients with relapsed adult T cell leukemia/lymphoma after allogeneic hematopoietic stem cell transplantation
Authors: Kato Koji , Uike Naokuni , Wake Atsushi , Yoshimitsu Makoto , Tobai Tomomi , Sawayama Yasushi , Takatsuka Yoshifusa , Fukuda Takahiro , Uchida Naoyuki , Eto Tetsuya , Nakashima Yasuhiro , Kondo Tadakazu , Taguchi Jun , Miyamoto Toshihiro , Nakamae Hirohisa , Ichinohe Tatsuo , Kato Koji , Suzuki Ritsuro , Utsunomiya Atae ,   ATL Working Group of the Japan Society for Hematopoietic Cell Transplantation
Published: September 2018

AbstractTreatment options for patients with adult T cell leukemia/lymphoma (ATLL) who have relapsed disease after allogeneic hematopoietic stem cell transplantation (allo‐HSCT) are limited. To clarify which patients with ATLL are likely to benefit from these treatment options and to define patient populations for novel treatments, we performed a nationwide retrospective analysis of 252 Japanese patients who had relapsed ATLL after allo‐HSCT. Some long‐term survivors remained after tapering and withdrawal of immunosuppressive agents. Thirty‐six patients who received donor lymphocyte infusion had a better overall survival (OS) in comparison to those who did not [hazard ratio (HR), 0.63; 95% confidence interval (CI), 0.43‐0.93; P = .02], suggesting the efficacy of a graft‐versus‐ATLL (GvATLL) effect even after relapse. Multivariate analysis demonstrated that skin lesions at initial relapse of ATLL were independently associated with higher OS (HR, 0.41; 95% CI, 0.22‐0.74; P = .003), indicating that the skin is a susceptible target organ of GvATLL. This study suggested that enhancement of a GvATLL effect is a potential therapeutic option for relapsed disease after allo‐HSCT. Further investigations of incorporation of immune‐based approaches with new molecular target drugs into the therapeutic options of patients with ATLL before and after transplantation are warranted.
A high‐fat diet impairs mitochondrial biogenesis, mitochondrial dynamics, and the respiratory chain complex in rat myocardial tissues
Authors: Chen Dan , Li Xia , Zhang LiTing , Zhu Mei , Gao Ling 
Published: September 2018

AbstractA high‐fat diet (HFD) has been associated with heart failure and arrhythmias; however, the molecular mechanisms underlying these associations are poorly understood. The mitochondria play an essential role in optimal heart performance, most of the energy for which is obtained from the oxidation of fatty acids. As such, chronic exposure to excess fatty acids may cause mitochondrial dysfunction and heart failure. To investigate the effects of a HFD on the mitochondrial function in the myocardium, 40 male rats were randomly divided into two groups and fed with either a normal diet or a HFD for 28 weeks. The myocardial lipid content, cardiac parameters and function, and mitochondrial morphology and function were evaluated. The expression of a number of genes involved in mitochondrial dynamics was measured using quantitative polymerase chain reaction and Western blot analyses. Proteomic analysis was also performed to identify the proteins affected by HFD treatment. Significant fat deposition in the myocardia, cardiac hypertrophy, and cardiac dysfunction were all observed in HFD‐treated rats. Electron microscopy showed abnormal mitochondrial density and morphology. In addition, abnormal expression of genes involved in mitochondrial dynamics, decreased mitochondrial DNA copy numbers, reduced complex I‐III and citrate synthase activities, and decreased mitochondrial respiration were observed in HFD‐treated rats. High performance liquid chromatography showed downregulated adenosine triphosphate (ATP) and adenosine diphosphate levels and an increased adenosine monophosphate (AMP)/ATP ratio. Proteomic analysis confirmed the alteration of mitochondrial function and impaired expression of proteins involved in mitochondrial dynamics in HFD‐treated rats. Mitochondrial dysfunction and impaired mitochondrial dynamics play an important role in heart dysfunction induced by a HFD, thus presenting a potential therapeutic target for the treatment of heart disease.
Opening of the CLC‐3 chloride channel induced by dihydroartemisinin contributed to early apoptotic events in human poorly differentiated nasopharyngeal carcinoma cells
Authors: Zhou Congran , Tang Xinwei , Xu Jingkui , Wang Jiajia , Yang Yaping , Chen Yehui , Chen Lixin , Wang Liwei , Zhu Linyan , Yang Haifeng 
Published: September 2018

AbstractNasopharyngeal carcinoma (NPC) is a specific type of head and neck cancer that is prevalent in Southeast Asia. Dihydroartemisinin (DHA), a semisynthetic derivative of artemisinin, has specific anticancer activity. Here, we aimed to investigate the role of the CLC‐3 chloride channel in the anticancer effect of DHA in poorly differentiated NPC CNE‐2Z cells. First, we observed that DHA could specifically inhibit the proliferation, induce apoptosis, and increase cleaved caspase‐3 expression in the CNE‐2Z cells. Then, we found that DHA could activate chloride channels, which led to Cl− efflux and apoptotic volume decrease (AVD) in the early stage in the CNE‐2Z cells. DHA also specifically increased CLC‐3 chloride channel protein expression in the CNE‐2Z cells. Silencing of the CLC‐3 protein expression depleted the Cl− currents, and decreased the AVD capacity and cell apoptosis induced by DHA. Finally, we revealed that the [Ca2+]i increased after around 6 hours of treatment with DHA, which was also inhibited by silencing of the CLC‐3 protein expression. Our data demonstrated that the selective antitumor activities of DHA in NPC may occur through the specific activation of the CLC‐3 Cl− channel, leading to Cl− efflux, and induced AVD, then led to [Ca2+]i accumulation and caspase‐3 activation, and finally induced apoptosis. The activation of the CLC‐3 chloride channel played an essential and proximal upstream role in the antitumor activities of DHA.
Genomic integrity of ground‐state pluripotency
Authors: Jafari Narges , Giehr Pascal , Hesaraki Mahdi , Baas Roy , de Graaf Petra , Timmers H. T. Marc , Walter Jörn , Baharvand Hossein , Totonchi Mehdi 
Published: September 2018

AbstractPluripotent cells appear to be in a transient state during early development. These cells have the capability to transition into embryonic stem cells (ESCs). It has been reported that mouse pluripotent cells cultivated in chemically defined media sustain the ground state of pluripotency. Because the epigenetic pattern of pluripotent cells reflects their environment, culture under different conditions causes epigenetic changes, which could lead to genomic instability. This study focused on the DNA methylation pattern of repetitive elements (REs) and their activation levels under two ground‐state conditions and assessed the genomic integrity of ESCs. We measured the methylation and expression level of REs in different media. The results indicated that although the ground‐state conditions show higher REs activity, they did not lead to DNA damage; therefore, the level of genomic instability is lower under the ground‐state compared with the conventional condition. Our results indicated that when choosing an optimum condition, different features of the condition must be considered to have epigenetically and genomically stable stem cells.
Decreased miR‐200a‐3p is a key regulator of renal carcinoma growth and migration by directly targeting CBL
Authors: Ding Meng , Sun Xinlei , Zhong Jinsha , Zhang Cuiping , Tian Yaping , Ge Jingping , Zhang Chen‐Yu , Zen Ke , Wang Jun‐Jun , Zhang Chunni , Wang Cheng 
Published: September 2018

AbstractAlthough emerging evidence has revealed that microRNAs (miRNAs) dysregulation contribute to carcinogenesis, the mechanism underlying their roles in renal cell carcinoma (RCC) is unclear. The purpose of the current study was to analyze the association of miR‐200a‐3p expression with RCC and to understand potential novel target genes, functions and mechanisms of miR‐200a‐3p in RCC. MiR‐200a‐3p expression levels were first measured by quantitative real‐time polymerase chain reaction and in situ hybridization in pairs of RCC tissue samples. Next, the potential miR‐200a‐3p target gene was analyzed using a combination of computer‐aided algorithms, luciferase reporter assays and Western blot analysis. Finally, the biological roles of miR‐200a‐3p in RCC tumorigenesis were investigated both in vitro by 5‐ethynyl‐20‐deoxyuridine, apoptosis assay and transwell assay, as well as in vivo using a xenograft mouse model. Our results demonstrated that miR‐200a‐3p was remarkably downregulated in RCC tissues compared with normal adjacent tissue, and CBL is a direct target of miR‐200a‐3p. An inverse correlation between miR‐200a‐3p and CBL was observed in RCC tissue samples. Mechanistic investigations revealed that ectopic expression of miR‐200a‐3p in RCC cell lines suppressed cell proliferation and migration and enforced cell apoptosis by directly inhibiting CBL in vitro and in vivo, whereas silencing miR‐200a‐3p resulted in the opposite effects. Additionally, overexpressing CBL abolished the effects induced by miR‐200a‐3p overexpression. Taken together, our results show that the miR‐200a‐3p/CBL regulation axis is a novel mechanism underlying RCC pathogenesis and may serve as a candidate biomarker and therapeutic target in RCC.
Developing DNA methylation‐based prognostic biomarkers of acute myeloid leukemia
Authors: Gao Chundi , Zhuang Jing , Zhou Chao , Liu Lijuan , Liu Cun , Li Huayao , Zhao Minzhang , Liu Gongxi , Sun Changgang 
Published: September 2018

AbstractAcute myeloid leukemia (AML) is a heterogeneous clonal neoplasm characterized by complex genomic alterations. The incidence of AML increases with age, and most cases experience serious illness and poor prognosis. To explore the relationship between abnormal DNA methylation and the occurrence and development of AML based on the Gene Expression Database (GEO), this study extracted data related to methylation in AML and identified a methylated CpG site that was significantly different in terms of expression and distribution between the primary cells of AML patients, and hematopoietic stem/progenitor cells from normal bone marrow. To further investigate the differences caused by the dysfunction of methylation sites, bioinformatics analysis was used to screen methylation‐related biomarkers, and the potential prognostic genes were selected by univariate and multivariate Cox proportional hazards regressions. Finally, five independent prognostic indicators were identified. In addition, these results provide new insight into the molecular mechanisms of methylation.
Evaluation of IL‐17A, IL‐17F, IL‐23R, VDR, CCL2, CCL5, CCR2, and CCR5 gene polymorphisms and expression in Chinese individuals with syphilis
Authors: Hu Wen‐Long , Ren Hong , Xu Bu‐Fang , Zhang Jing‐Ping , Zhang Rui‐Li , Wang Qian‐Qiu , Zhang Ting‐Ting 
Published: September 2018

AbstractSyphilis is a sexually transmitted disease caused by the infection of Treponema pallidum subspecies pallidum. T‐helper type 17–related genes, vitamin D receptor (VDR) gene, and chemokine/chemokine receptor genes are crucial in microbial infection. A total of 16 single‐nucleotide polymorphisms (SNPs) in eight genes (interleukin [IL]‐17A, IL‐17F, IL‐23R, VDR, C‐C motif chemokine ligand [CCL] 2, CCL5, C‐C chemokine receptor [CCR] 2, and CCR5) were analyzed in 188 patients with syphilis and 216 healthy controls. The results showed a strong correlation of IL‐17A rs2275913 (AA vs AG + GG: odds ratio [OR], 1.78; 95% confidence interval [CI], 1.09 to 2.92; P = 0.020; A vs G: OR, 1.33; 95% CI, 1.01 to 1.76; P = 0.043) and rs3819024 (GG vs AA + GA: OR, 1.76; 95% CI, 1.06 to 2.91; P = 0.028; G vs A: OR, 1.36; 95% CI, 1.03 to 1.80; P = 0.030) with syphilis. In haplotype analysis, IL‐17A rs2275913A/rs3819024G showed a risk effect (OR, 1.38; 95% CI, 1.04 to 1.82; P = 0.026), whereas IL‐17A rs2275913G/rs3819024A showed a protective effect (OR, 0.76; 95% CI, 0.57 to 0.998; P = 0.048). The expression levels of IL‐17A messenger RNA (mRNA) in peripheral blood mononuclear cells and IL‐17A secretion in plasma were further examined. No significant differences were found between patients with syphilis and healthy controls. The study also explored whether IL‐17A rs2275913 and rs3819024 were associated with the expression of IL‐17A mRNA and IL‐17A secretion in patients with syphilis. Similar negative results were found. In conclusion, the polymorphisms of IL‐17A rs2275913 and rs3819024 and the haplotype containing these two SNPs influenced the susceptibility to syphilis in a Han Chinese population.
Perillaldehyde prevents the formations of atherosclerotic plaques through recoupling endothelial nitric oxide synthase
Authors: Yu Li , Liu Hua 
Published: September 2018

AbstractBackgroundsNitric oxide (NO) produced by endothelial NO synthase (eNOS) is essential for cardiovascular homeostasis owing to its anti‐inflammatory, antithrombotic, antiproliferative, and antioxidant effects. Perillaldehyde (PAH), one of the major oil components in Perilla frutescens, has anti‐inflammatory effects.AimThis study was designed to explore whether PAH prevents atherosclerosis through normalizing eNOS functions.MethodsThe model of atherosclerosis was induced by feeding rats and mice with high‐fat diet or plus balloon injury. Oil red and hematoxylin‐eosin (HE) stainings were used to assess the atherosclerotic plaque.ResultsPretreatment of PAH for 30 minutes concentration‐dependently increased tetrahydrobiopterin (BH4) levels, NO generations, and improved cell viabilities in cultured human umbilicus vessel endothelial cells (HUVEC) incubated with oxidized (OX) low‐density lipoprotein (LDL), in which all protective effects of PAH were abolished by guanosinetriphosphate (GTP) cyclohydrolase 1 inhibitor 2,4‐Diamino‐6‐hydroxypyrimidine (DAHP) or eNOS inhibitor L‐NAME in those cells. In rats, high‐fat diet plus balloon injury induced the formation of atherosclerotic plaque in carotid arteries. Administration of PAH or lovastatin reduced the size of atherosclerotic plaque in rats and improved the responses of aortic rings to acetylcholine isolated from rats, accompanied with increased BH4 content and NO generations. In Apoe
−/− mice feeding with normal diet or high‐fat diet, PAH (150 mg/kg) reduced the size of atherosclerotic plaque in aortic arteries, prevented endothelial dysfunctions, and increased both BH4 and NO generations in carotid arteries.ConclusionPAH prevents the growth of atherosclerosis through increasing BH4 generation and subsequent eNOS recouping. Clinically, PAH should be considered as a new medicine to treat patients with atherosclerosis.
Effect of scrotal heating on sperm quality, seminal biochemical substances, and reproductive hormones in human fertile men
Authors: Zhang Mei‐Hua , Zhai Li‐Ping , Fang Zhen‐Ya , Li An‐Na , Xiao Wei , Qiu Yi 
Published: September 2018

AbstractAt present, male contraceptive methods are only vasectomy and condoms, so it is necessary to research on male contraceptive techniques. The aim of this study is to observe the effects of scrotal heating (SH) on semen parameters, seminal l‐carnitine (LC), epidermal growth factor (EGF), macrophage migration inhibitory factor (MIF), reproductive hormones and sperm chromosome numbers of adult healthy men, and to provide the experimental data for male contraception. The scrotums of 30 healthy male volunteers were exposed to the condition of 40 to 43°C SH belt warming 40 minutes each day for successive 2 days per week. The course of SH was continuous for 3 months. Computer‐assisted semen analysis and hypo‐osmotic swelling test, sperm DNA integrity, l‐carnitine, MIF and EGF, and sperm fluorescence in situ hybridization were performed before, during, and after SH. The serum level of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) were measured by chemiluminescent immunoassay. The mean parameters of sperm concentration, vitality, and normal morphological sperm were significantly decreased in groups with sperms being collected during 1, 2, and 3 months of SH when compared with those in groups of pre‐SH (P < 0.01). Statistically significant differences of sperm DNA fragmentation, normal sperm membrane functionality, levels of LC and MIF in semen, and LH, FSH, and T in serum were observed between the groups of before SH and after SH 3 months and the groups of during SH 1, 2, and 3 months (P < 0.001). The total rate of chromosome number for 13, 18, 21, X, and Y in the 3 months of SH was 13.7‐fold greater (13.72%/1.69%) than before SH (P < 0.001). The constant SH can impact the semen quality, sperm DNA integrity, sperm chromosome, LC and MIF, and LH, FSH, and T in serum. Transient SH may be a new method for male contraception.
Characterization of potential TRPP2 regulating proteins in early Xenopus embryos
Authors: Futel Mélinée , Le Bouffant Ronan , Buisson Isabelle , Umbhauer Muriel , Riou Jean‐François 
Published: September 2018

AbstractTransient receptor potential cation channel‐2 (TRPP2) is a nonspecific Ca2+‐dependent cation channel with versatile functions including control of extracellular calcium entry at the plasma membrane, release of intracellular calcium ([Ca2+]i) from internal stores of endoplasmic reticulum, and calcium‐dependent mechanosensation in the primary cilium. In early Xenopus embryos, TRPP2 is expressed in cilia of the gastrocoel roof plate (GRP) involved in the establishment of left‐right asymmetry, and in nonciliated kidney field (KF) cells, where it plays a central role in early specification of nephron tubule cells dependent on [Ca2+]i signaling. Identification of proteins binding to TRPP2 in embryo cells can provide interesting clues about the mechanisms involved in its regulation during these various processes. Using mass spectrometry, we have therefore characterized proteins from late gastrula/early neurula stage embryos coimmunoprecipitating with TRPP2. Binding of three of these proteins, golgin A2, protein kinase‐D1, and disheveled‐2 has been confirmed by immunoblotting analysis of TRPP2‐coprecipitated proteins. Expression analysis of the genes, respectively, encoding these proteins, golga2, prkd1, and dvl2 indicates that they are likely to play a role in these two regions. Golga2 and prkd1 are expressed at later stage in the developing pronephric tubule where golgin A2 and protein kinase‐D1 might also interact with TRPP2. Colocalization experiments using exogenously expressed fluorescent versions of TRPP2 and dvl2 in GRP and KF reveal that these two proteins are generally not coexpressed, and only colocalized in discrete region of cells. This was observed in KF cells, but does not appear to occur in the apical ciliated region of GRP cells.