bluuebunny/crossref_metadata_2025_split huggingface dataset:
The E3 ligase OsPUB33 controls rice grain size and weight by regulating the OsNAC120–BG1 module
Authors: Xie Zizhao , Sun Ying , Zhan Chenghang , Qu Chengfeng , Jin Ning , Gu Xinyue , Huang Junli 
Published: November 2024

Abstract
Grain size and weight are important determinants of crop yield. Although the ubiquitin pathway has been implicated in the grain development in rice (Oryza sativa), the underlying genetic and molecular mechanisms remain largely unknown. Here, we report that the plant U-box E3 ubiquitin ligase OsPUB33 interferes with the OsNAC120–BG1 module to control rice grain development. Functional loss of OsPUB33 triggers elevated photosynthetic rates and greater sugar translocation, leading to enhanced cell proliferation and accelerated grain filling. These changes cause enlarged spikelet hulls, thereby increasing final grain size and weight. OsPUB33 interacts with transcription factor OsNAC120, resulting in its ubiquitination and degradation. Unlike OsPUB33, OsNAC120 promotes grain size and weight: OsNAC120-overexpression plants harbor large and heavy grains, whereas osnac120 loss-of-function mutants produce small grains. Genetic interaction analysis supports that OsPUB33 and OsNAC120 function at least partially in a common pathway to control grain development, but have opposite functions. Additionally, OsNAC120 transcriptionally activates BIG GRAIN1 (BG1), a prominent modulator of grain size, whereas OsPUB33 impairs the OsNAC120-mediated regulation of BG1. Collectively, our findings uncover an important molecular framework for the control of grain size and weight by the OsPUB33–OsNAC120–BG1 regulatory module and provide promising targets for improving crop yield.
cunuSHT: GPU accelerated spherical harmonic transforms on arbitrary pixelizations
Authors: Belkner Sebastian , Duivenvoorden Adriaan J , Carron Julien , Schaeffer Nathanael , Reinecke Martin 
Published: November 2024

ABSTRACT
We present cunuSHT, a general-purpose Python package that wraps a highly efficient CUDA implementation of the non-uniform spin-0 spherical harmonic transform. The method is applicable to arbitrary pixelization schemes, including schemes constructed from equally spaced iso-latitude rings as well as completely non-uniform ones. The algorithm has an asymptotic scaling of $\mathcal {O}{(\ell _{\rm max}^3)}$ for maximum multipole $\ell _{\rm max}$ and can be made to achieve machine precision accuracy, considering band-limited transforms for which $N\approx \ell _{\rm max}^2$ (where N is the number of pixels in the map). While cunuSHT is developed for applications in cosmology in mind, it is applicable to various other interpolation problems on the sphere. We outperform the fastest available CPU algorithm at problem sizes $\ell _{\rm max}\sim 4\times 10^2$ and larger. The speed-up increases with the problem size and reaches a factor of up to 5 for problems with a non-uniform pixelization and $\ell _{\rm max}\gt 4\times 10^3$ when comparing a single modern GPU to a modern 32-core CPU. This performance is achieved by utilizing the double Fourier sphere method in combination with the non-uniform fast Fourier transform and by avoiding transfers between the host and device. For scenarios without GPU availability, cunuSHT wraps existing CPU libraries. cunuSHT is publicly available and includes tests, documentation, and demonstrations.
Alternativizing markets: the framing of moral commerce
Authors: Bååth Jonas , Fuentes Christian 
Published: November 2024

Abstract
How are moral markets organized? While previous research has shown that moral markets combine conventional and alternative elements, it has failed to explain how these hybridizations can ensure the organization of moral markets. To this end, we take an economic-sociological approach using ethnographic materials from a study of Swedish Reko-rings, a direct-to-customer market for local foods, to analyse how actors address the key coordination problems of value, competition and cooperation. The analysis shows how participants of these markets enact an ‘alternative framing’, emphasizing economic certainty as instrumental for moral certainty, which enables them to develop and implement ‘alternativized’ solutions to market coordination problems. Thereby, participants make economic and moral values certain by making the former instrumental for the latter. We advance previous research by showing that moral markets require not just hybridization, but hybridization guided by a specific economic-moral framing that enables these markets to avoid co-option.
Estate planning for art collections—a German perspective
Authors: Garbers-von Boehm Katharina , Neziraj Betim 
Published: November 2024

Abstract
Under German law, artworks are highly attractive assets when it comes to estate planning for various reasons: First, the valuation method to be used is favourable, secondly, if applicable, payment of taxes with artworks instead of cash can ease liquidity issues, and thirdly, donations to public-benefit organizations can significantly reduce tax liabilities. Last but not least, artworks in private collections may be eligible for tax exemptions of either 60% or 100%. In the course of such estate planning in relation to artworks, it is important to consider certain provisions of inheritance law to avoid disputes among the future heirs and, if the testator wishes so, to protect the integrity of the art collection.
Association between eating rate and childhood overweight/obesity: a systematic review and meta-analysis
Authors: Fang Kehong , Liu Hui , Zhai Bingzhong , Wang Lingli , Zhao Lijuan , Hao Li , Huang Liming , Zhang Xuhui 
Published: November 2024

Abstract
Many studies have reported the relationship between eating rate and childhood overweight/obesity, while results remain inconclusive. The present study was done to estimate the association between eating rate and childhood overweight/obesity through a systematic review of prevalence studies. Relevant studies were searched by two independent researchers in databases including PubMed, Embase, Cochrane Library, and Web of Science, and data were collected from relevant studies published through June 2023 using predefined inclusion/exclusion criteria. A summary estimate was calculated using a random-effect model, and subgroup analysis was performed to explore sources of heterogeneity. Data from 16 published studies were eligible for inclusion. Fast eating was associated with a higher risk of overweight/obesity compared with a medium eating rate (OR = 1.80; 95% CI: 1.49, 2.18), and slow eating showed a declined overweight/obesity risk among children and adolescents (OR = 0.65; 95% CI: 0.52, 0.81). Subgroup analysis performed according to age showed that in all age groups, eating fast was positively correlated with overweight/obesity, while eating slowly was negatively associated with overweight/obesity. According to our study, eating rate was closely related to childhood overweight/obesity, and eating fast was associated with an increased likelihood of being overweight/obesity. In the future, it will be necessary to understand the factors that influence fast eating and develop methods to slow down the eating rate in children and adolescents.
Maternal Mortality in the United States: Are the High and Rising Rates Due to Changes in Obstetrical Factors, Maternal Medical Conditions, or Maternal Mortality Surveillance?
Authors: Joseph K.S. , Lisonkova Sarka , Boutin Amélie , Muraca Giulia M. , Razaz Neda , John Sid , Sabr Yasser , Chan Wee-Shian , Mehrabadi Azar , Brandt Justin S. , Schisterman Enrique F. , Ananth Cande V. 
Published: November 2024

(Abstracted from Am J Obstet Gynecol 2024;230:440.e1–440.e13)
High reported maternal death rates in the United States have garnered attention and skepticism, especially with reports indicating a near doubling of maternal mortality between 2018 and 2021. Some attribute this increase to changes in reporting criteria for maternal mortality developed in 2003 by the National Center for Health Statistics (NCHS).
Enhancing Perioperative Outcomes of Pancreatic Surgery with Wearable Augmented Reality Assistance System: A Matched-Pair Analysis
Authors: Javaheri Hamraz , Ghamarnejad Omid , Widyaningsih Rizky , Bade Ragnar , Lukowicz Paul , Karolus Jakob , Stavrou Gregor Alexander 
Published: November 2024

Objective:
The present study aimed to evaluate the safety of the first wearable augmented reality assistance system (ARAS) specifically designed for pancreatic surgery and its impact on perioperative outcomes.

Background:
Pancreatic surgery remains highly complex and is associated with a high rate of perioperative complications. ARAS, as an intraoperative assistance system, has the potential to reduce these complications.

Methods:
This prospective, single-center study included 20 patients who underwent pancreatic surgery using ARAS. These patients were matched in a 1:3 ratio with 60 patients from our retrospective data who underwent standard pancreatic resection. Matching variables were selected based on factors associated with poor intraoperative outcomes.

Results:
A higher proportion of patients in the ARAS group were diagnosed with borderline resectable pancreatic cancer and received neoadjuvant chemotherapy (20.0% vs 6.7%, P = 0.085). Additionally, more patients in the ARAS group required arterial resection compared with the control group (15.0% vs 0.0%, P = 0.002). Nevertheless, the ARAS group had a significantly shorter operative time (246 vs 299 minutes, P = 0.004) and required significantly fewer intraoperative blood transfusions (0.0 ± 0.0 vs 0.5 ± 1.4 units, P = 0.014). None of the patients in the ARAS group had positive resection margins (0.0% vs 20.0%, P = 0.045). Furthermore, patients in the ARAS group experienced a significantly shorter hospital stay (13.8 ± 6.6 vs 17.9 ± 8.2 days, P = 0.046).

Conclusions:
ARAS is a safe and effective assistance system for pancreatic surgery, offering superior perioperative outcomes compared with standard procedures.
Evaluating Advanced Machine Learning Models for Histopathological Diagnosis of Hansen Disease
Authors: Vargas-Clavijo Mariana , Cardona-Castro Nora , Ospina-Gómez Juan Pablo , Serrano-Coll Héctor 
Published: November 2024

Introduction:
Leprosy is a neglected infectious disease caused by Mycobacterium leprae and Mycobacterium lepromatosis and remains a public health challenge in tropical regions. Therefore, the development of technological tools such as machine learning (ML) offers an opportunity to innovate strategies for improving the diagnosis of this complex disease.

Objective:
To validate the utility of different ML models for the histopathological diagnosis of Hansen disease.

Methodology:
An observational study was conducted where 55 H&E-stained tissue slides from leprosy patients and 51 healthy skin controls were analyzed using microphotographs captured at various magnifications. These images were categorized based on histopathological findings and processed using the Cross-Industry Standard Process for Data Mining methodology for designing and training ML models. Five types of ML models were evaluated using standard metrics such as accuracy, sensitivity, and specificity, emphasizing data normalization as a fundamental step in optimizing model performance.

Results:
The artificial neural network (ANN) model demonstrated an accuracy of 70%, sensitivity of 74%, and specificity of 65%, demonstrating its ability to identify leprosy cases with moderate precision. The receiver operating characteristic curve of the ANN model showed an area under the curve of 0.71. Conversely, models such as decision trees, logistic regression, and random forests showed similar accuracy results but with slightly lower sensitivity, potentially indicating a higher risk of false negatives in detecting leprosy-positive cases.

Conclusion:
The ANN model emerges as a promising alternative for leprosy detection. However, further refinement of these models is necessary to enhance their adaptability across different clinical settings and participation in patient care.
Quantifying Mast Cell and Eosinophil Cellular Density in Skin Biopsy Tissue From Adults With Maculopapular Cutaneous Mastocytosis as Compared With Urticaria and Normal Skin: A Retrospective Histopathologic Study
Authors: King Anne L. , Montagnon Carmen M. , Todd Austin , Agrawal Shruti , Wieland Carilyn N. , Lehman Julia S. , Johnson Emma F. 
Published: November 2024

Background:
Maculopapular cutaneous mastocytosis (MPCM) is a rare disorder characterized by a pathologic accumulation of mast cells in the skin, which may or may not be accompanied by systemic mastocytosis. Diagnosis of MPCM on skin biopsy can be challenging because the findings may be subtle. Although mast cell density in MPCM has been reported, data informing a proposed cutoff for diagnosis and diagnostic criteria are limited.

Methods:
We identified adult patients diagnosed with MPCM and urticarial tissue reaction/chronic urticaria on skin biopsy and compared the mast cell and eosinophil counts per 1 mm2 in 10 cases each of MPCM, chronic urticaria, and normal skin from routine biopsies. All slides were stained with CD117, and CD117-positive mast cells were counted per 1 mm2 using digital microscopy. Eosinophils were counted on hematoxylin and eosin-stained slides per 1 mm2 using digital microscopy.

Results:
The median number of mast cells per 1 mm2 was significantly higher in MPCM than in cases of urticaria and normal skin/control tissue (177.3 vs. 26.8 vs. 47.8 mast cell per mm2, respectively; P ≤ 0.001). The calculated “cut point” for mastocytosis versus chronic urticaria and normal skin was 66 mast cells per 1 mm2, whereas the value for controls versus urticaria was 37 mast cells per 1 mm2. Eosinophils had similar density in MPCM and urticaria, and their presence was significant in the differentiation of MPCM and urticaria from normal tissue.

Conclusions:
This study adds to the literature by providing objective mast cell density data to distinguish challenging cases of cutaneous mastocytosis from urticarial reactions and normal skin. Future studies could explore the development of computer-aided estimations of cellular density with more extensive comparison with other inflammatory conditions to translate our findings more readily into clinical practice.
Ossifying Panniculitis Presenting as a Painful Nodule on the Abdomen
Authors: Oaks Rosemary , Beard Abigail , Miller Richard , Baribault Keith 
Published: November 2024

Abstract:
Myositis ossificans is a benign ossifying nodule that can affect any type of tissue but is most commonly found in muscle. Occurrence in the soft tissue of the subcutis is less common and has been referred to as panniculitis ossificans. In this case report, we describe a 46-year-old woman who presented with a 1.7 × 2.0 cm lesion on the abdomen that had been present for several months, was painful, and easily irritated by clothing. Initial clinical assessment based on history and physical examination was an epidermal cyst and removal by excision was performed. Initial histologic findings showed a spindle cell proliferation in the subcutis along with bone formation. Osteoblasts were noted to be rimming the bone and giant cells were present. The spindle cells were SMA positive and partially factor XIIIa positive while S100 protein staining was negative, confirming the diagnosis of panniculitis ossificans.
First bipolar episode following booster dose of COVID-19 vaccination
Authors: Kudsi Maysoun , Tarcha Raghad , Khalayli Naram , Hodaifa Yara , Alghawe Fatima Alzahraa 
Published: November 2024

Introduction and importance:
The interactions between the susceptibility of the host and some of the COVID-19 vaccine components happened. Viral infections, including COVID-19, are related to the pathogenesis of bipolar disorder (BD). These findings could trigger an initial bipolar episode.

Case presentation:
A 48-year-old Syrian married female, presented to the out-clinic of Modern Medical Centre Hospital, in July 2022 for episodes of sub-maniac/mania episodes, which began within 15 days after the booster dose of COVID-19 vaccination, preceded by depressive mood. Both family and personal past psychiatric and/or trauma history were negatives. Initiation of carbamazepine 600 mg/day showed significant improvement within 3 days; 3 months later, carbamazepine dose was decreased to 200 mg/day, as there were no clinical symptoms.

Clinical discussion:
mRNA vaccines of COVID-19 increase the levels of type I interferon (INF), an inflammatory cytokine that creates a signal leading to the expression of interleukins, INF, and tumor necrosis factor-alpha. The pathology of BD is also unclear. Genetic predisposition, immunological effects, and the classification of BD, itself, as an autoimmune disease play a role. Although rapid improvement following treatment, prolonged and maintenance treatment by essentially mood stabilizer should be considered in bipolar episodes initiated by COVID-19 vaccination. Rare data are available about maniac episodes after COVID-19 infection, but to our knowledge, we reported the first case of bipolar onset after the booster vaccination of COVID-19.

Conclusion:
Bipolar disease can present following a booster dose of COVID-19 vaccination in previously healthy people.
Repair of Scrotal Wounds after Extensive Excision of Extramammary Paget Disease with Thick Split-thickness Skin Graft
Authors: Chen Yi , Tao Ke-Rong , Luo Zhao-He , Sun Guang-Feng 
Published: November 2024

Summary:
Extramammary Paget disease (EMPD) is a rare cutaneous malignant tumor. Currently, surgery is the most recommended treatment for EMPD. Here, we present a case of EMPD involving the groin, penis, and scrotum. The patient underwent two lesion resections. A thick split-thickness skin graft was utilized for scrotal reconstruction. After a 37-month follow-up period, no tumor recurrence was detected, and the outcome was deemed satisfactory.
The Effect of Breathing and Relaxation Exercises on Self-Care, Dyspnea, and Fatigue in Heart Failure
Authors: Solmaz Gürcan , Mollaoğlu Mukadder , Asoğlu Ramazan 
Published: November 2024

Dyspnea, fatigue, and self-care are common occurrences of heart failure. This study evaluated the effects of yoga breathing and progressive muscle relaxation exercises on dyspnea, fatigue, and self-care in heart failure. The study conducted between April 2023 and March 2024 randomized 66 heart failure patients into 3 groups (yoga breathing exercise, progressive muscle relaxation exercise, and control). The intervention groups engaged in specific exercises tailored to their assigned regimen for 10 minutes twice daily over a 2-week period, while the control group maintained their pharmacological treatment. At the end of the intervention (second week) yoga breathing exercise notably ameliorated fatigue, dyspnea, and self-care (P< .001), changes in saturation, heart rate, and respiratory rate favoring the intervention groups (P< .05). Yoga breathing exercises enhance self-care, alleviate fatigue, and dyspnea in heart failure. Additionally, yoga breathing and progressive muscle relaxation exercises provide benefits in regulating saturation, heart rate, and respiratory rate in heart failure.
Genetic Evidence Supporting a Causal Role of Snoring in Keratoconus: A Bidirectional Mendelian Randomization Study
Authors: Chen Xi , Liu Shiji , Liu Chang , Huang Yuke , Hou Xiangtao , Zhuang Jiejie , Luo Yiqi , Yu Na , Zhuang Jing , Yu Keming 
Published: November 2024

Purpose:
To clarify the controversial causal association between snoring and keratoconus (KCN), which is crucial in clinical prevention and treatment.

Methods:
This is a 2-sample bidirectional mendelian randomization (MR) case–control study. MR is an innovative method that uses genetic variation as a natural experiment to investigate the causal relationships between potentially modifiable risk factors and health outcomes in observational data. The single nucleotide polymorphisms associated with snoring were retrieved from the UK biobank cohort with 218,346 participants (61,792 cases and 156,554 controls). The summary statistics of KCN were obtained from the European ancestry with 209,598 subjects (311 cases and 209,287 controls). The inverse-variance-weighted method was applied as the primary estimate, whereas weighted median and MR-pleiotropy residual sum and outlier played a subsidiary role.

Results:
Elevated risk of snoring showed a robust causal effect on KCN (inverse-variance-weighted: causal effect = 9.821, 95% confidence interval [CI], 1.944–17.699, P = 0.015), which was consistent with complementary methods of the weighted median (causal effect = 11.117, 95% CI, 2.603–19.631, P = 0.010), maximum likelihood (causal effect = 10.245, 95% CI, 3.967–16.523, P = 0.001), and MR-pleiotropy residual sum and outlier (causal effect = 9.793, 95% CI, 2.316–17.269, P = 0.028). However, there was no causality of KCN on the increasing risk of snoring.

Conclusions:
This study provides genetic evidence supporting the causal role of snoring on KCN. Our findings provide new insights into potential strategies to manage KCN.
Shock wave-pretreated ADMSCs of cell-sheet scaffold (CSS) patched on the left ventricular wall (LVW) inhibited LVW remodeling in mini-pig MI: role of CSS on counteracting Laplace’s Law of LVW stress – experimental study
Authors: Sheu Jiunn-Jye , Yeh Jui-Ning , Chen Yin-Chia , Chiang John Y. , Sung Pei-Hsun , Huang Chi-Ruei , Li Yi-Chen , Yip Hon-Kan 
Published: November 2024

Background:
We investigated whether shock wave (SW)-pretreated autologous adipocyte-derived mesenchymal stem cells (ADMSCs) seeded in the cell-sheet scaffold (CSS) could inhibit left ventricular (LV) remodeling and improve LV ejection fraction (LVEF) in old myocardial infarction (MI).

Methods:
Mini-pigs (n=20) were divided into group 1 (sham-operated control), group 2 (old MI), group 3 (old MI + autologous ADMSCs/1.0×107 in CSS on LV myocardium), and group 4 [old MI + SW (0.12 mJ/mm2 for total 140 shots)-pretreated ADMSCs in CSS on LV myocardium]. Treatments started on day 28 after MI induction. In-vivo and in-vitro studies were conducted.

Results:
Cell viability/relative mitochondria DNA expression/mitochondrial cytochrome C/adenosine triphosphate concentration in ADMCSs and protein expressions of angiogenesis factors [vascular endothelial growth factor (VEGF)/stromal cell-derived factor-1 (SDF-1)/mitochondrial respiratory chain complexes I–IV/oxygen consumption rate] were higher in group 4 than in group 3 (P<0.001). By day 180, LVEF and small vessel numbers in the peri-infarct or infarct area were highest in group 1, lowest in group 2, and significantly lower in group 3 than in group 4. In contrast, the LV dimension was opposite to the pattern of change in LVEF in all groups (P<0.0001). The basal/middle/apical infarct and fibrotic areas were inversely related to LVEF in all groups (all P<0.0001). Protein levels of angiogenetic markers (SDF-1α/C-X-C chemokine receptor type 4/VEGF/angiopoietin-1) were significantly and persistently increased from groups 1 to 4. In contrast, protein levels of endothelial cell markers (von Willebrand factor or endothelial nitric oxide synthase) showed an identical pattern to LVEF in all groups (all P<0.0001).

Conclusion:
SW pretreatment of ADMSCs seeded in CSS offered significant benefits in preserving LV performance and ameliorating LV remodeling in mini-pigs with old MI.