bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental reduction of host Plasmodium infection load affects mosquito survival
Authors: Gutiérrez-López Rafael , Martínez-de la Puente Josué , Gangoso Laura , Yan Jiayue , Soriguer Ramón , Figuerola Jordi 
Published: June 2019

AbstractPlasmodium transmission success depends upon the trade-off between the use of host resources to favour parasite reproduction and the negative effects on host health, which can be mediated by infection intensity. Despite its potential influence on parasite dynamics, the effects of infection intensity on both, birds and vectors, and on Plasmodium transmission success are still poorly understood. Here, we experimentally reduced the Plasmodium load in naturally infected wild house sparrows with the antimalarial primaquine to assess the effects of intensity of infection in the vertebrate hosts on Plasmodium transmission to and by mosquitoes. We monitored the survival of Culex pipiens mosquitoes throughout the development of the parasite and the infection status of the mosquitoes by analysing the head-thorax and saliva at 13 days post-exposure to birds. The proportion of mosquitoes infected by Plasmodium and the presence of Plasmodium in saliva were not associated with the medication treatment of birds. However, the experimental treatment affected vector survival with mosquitoes fed on medicated birds showing a higher survival rate than those fed on control individuals. These results provide strong experimental evidence of the impact of parasite load of vertebrate hosts on the survival probability of malaria vectors.
Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection
Authors: Petrova Irina , Konopsky Valery , Nabiev Igor , Sukhanova Alyona 
Published: June 2019

AbstractCirculating cancer markers are metabolic products found in body fluids of cancer patients, which are specific for a certain type of malignant tumors. Cancer marker detection plays a key role in cancer diagnosis, treatment, and disease monitoring. The growing need for early cancer diagnosis requires quick and sensitive analytical approaches to detection of cancer markers. The approach based on the photonic crystal surface mode (PC SM) detection has been developed as a label-free high-precision biosensing technique. It allows real-time monitoring of molecular and cellular interactions using independent recording of the total internal reflection angle and the excitation angle of the PC surface wave. We used the PC SM technique for simultaneous detection of the ovarian cancer marker cancer antigen 125 and two breast cancer markers, human epidermal growth factor receptor 2 and cancer antigen 15-3. The new assay is based on the real-time flow detection of specific interaction between the antigens and capture antibodies. Its particular advantage is the possibility of multichannel recording with the same chip, which can be used for multiplexed detection of several cancer markers in a single experiment. The developed approach demonstrates high specificity and sensitivity for detection of all three biomarkers.
Realization of p-type gallium nitride by magnesium ion implantation for vertical power devices
Authors: Shi Ya-Ting , Ren Fang-Fang , Xu Wei-Zong , Chen Xuanhu , Ye Jiandong , Li Li , Zhou Dong , Zhang Rong , Zheng Youdou , Tan Hark Hoe , Jagadish Chennupati , Lu Hai 
Published: June 2019

AbstractImplementing selective-area p-type doping through ion implantation is the most attractive choice for the fabrication of GaN-based bipolar power and related devices. However, the low activation efficiency of magnesium (Mg) ions and the inevitable surface decomposition during high-temperature activation annealing process still limit the use of this technology for GaN-based devices. In this work, we demonstrate successful p-type doping of GaN using protective coatings during a Mg ion implantation and thermal activation process. The p-type conduction of GaN is evidenced by the positive Seebeck coefficient obtained during thermopower characterization. On this basis, a GaN p-i-n diode is fabricated, exhibiting distinct rectifying characteristics with a turn-on voltage of 3 V with an acceptable reverse breakdown voltage of 300 V. Electron beam induced current (EBIC) and electroluminescent (EL) results further confirm the formation of p-type region due to Mg ion implantation and subsequent thermal activation. This repeatable and uniform manufacturing process can be implemented in mass production of GaN devices for versatile power and optoelectronic applications.
Long-term impacts of rising sea temperature and sea level on shallow water coral communities over a ~40 year period
Authors: Brown B. E. , Dunne R. P. , Somerfield P. J. , Edwards A. J. , Simons W. J. F. , Phongsuwan N. , Putchim L. , Anderson L. , Naeije M. C. 
Published: June 2019

AbstractEffects of combined rising sea temperature and increasing sea level on coral reefs, both factors associated with global warming, have rarely been addressed. In this ~40 y study of shallow reefs in the eastern Indian Ocean, we show that a rising relative sea level, currently estimated at ~11 mm y−1, has not only promoted coral cover but also has potential to limit damaging effects of thermally-induced bleaching. In 2010 the region experienced the most severe bleaching on record with corals subject to sea temperatures of >31 °C for 7 weeks. While the reef flats studied have a common aspect and are dominated by a similar suite of coral species, there was considerable spatial variation in their bleaching response which corresponded with reef-flat depth. Greatest loss of coral cover and community structure disruption occurred on the shallowest reef flats. Damage was less severe on the deepest reef flat where corals were subject to less aerial exposure, rapid flushing and longer submergence in turbid waters. Recovery of the most damaged sites took only ~8 y. While future trajectories of these resilient reefs will depend on sea-level anomalies, and frequency of extreme bleaching the positive role of rising sea level should not be under-estimated.
Tuning properties of biomimetic magnetic nanoparticles by combining magnetosome associated proteins
Authors: Peigneux Ana , Jabalera Ylenia , Vivas Ma Antonia Fernández , Casares Salvador , Azuaga Ana I. , Jimenez-Lopez Concepción 
Published: June 2019

AbstractThe role of magnetosome associated proteins on the in vitro synthesis of magnetite nanoparticles has gained interest, both to obtain a better understanding of the magnetosome biomineralization process and to be able to produce novel magnetosome-like biomimetic nanoparticles. Up to now, only one recombinant protein has been used at the time to in vitro form biomimetic magnetite precipitates, being that a scenario far enough from what probably occurs in the magnetosome. In the present study, both Mms6 and MamC from Magnetococcus marinus MC-1 have been used to in vitro form biomimetic magnetites. Our results show that MamC and Mms6 have different, but complementary, effects on in vitro magnetite nucleation and growth. MamC seems to control the kinetics of magnetite nucleation while Mms6 seems to preferably control the kinetics for crystal growth. Our results from the present study also indicate that it is possible to combine both proteins to tune the properties of the resulting biomimetic magnetites. In particular, by changing the relative ratio of these proteins, better faceted and/or larger magnetite crystals with, consequently, different magnetic moment per particle could be obtained. This study provides with tools to obtain new biomimetic nanoparticles with a potential utility for biotechnological applications.
The antifungal potential of (Z)-ligustilide and the protective effect of eugenol demonstrated by a chemometric approach
Authors: Rodrigues Alice M. S. , Eparvier Véronique , Odonne Guillaume , Amusant Nadine , Stien Didier , Houël Emeline 
Published: June 2019

AbstractMankind is on the verge of a postantibiotic era. New concepts are needed in our battle to attenuate infectious diseases around the world and broad spectrum plant-inspired synergistic pharmaceutical preparations should find their place in the global fight against pathogenic microorganisms. To progress towards the discovery of potent antifungal agents against human pathologies, we embarked upon developing chemometric approach coupled with statistical design to unravel the origin of the anticandidal potential of a set of 66 essential oils (EOs). EOs were analyzed by GC-MS and tested against Candida albicans and C. parapsilosis (Minimal Inhibitory Concentration, MIC). An Orthogonal Partial Least Square (OPLS) analysis allowed us to identify six molecules presumably responsible for the anticandidal activity of the oils: (Z)-ligustilide, eugenol, eugenyl acetate, citral, thymol, and β-citronellol. These compounds were combined following a full factorial experimental design approach in order to optimize the anticandidal activity and selectivity index (SI = IC50(MRC5 cells)/MIC) through reconstituted mixtures. (Z)-Ligustilide and citral were the most active compounds, while (Z)-ligustilide and eugenol were the two main factors that most contributed to the increase of the SI. These two terpenes can, therefore, be used to construct bioinspired synergistic anticandidal mixtures.
Evaluation of Neurosecretome from Mesenchymal Stem Cells Encapsulated in Silk Fibroin Hydrogels
Authors: Martín-Martín Yolanda , Fernández-García Laura , Sanchez-Rebato Miguel H. , Marí-Buyé Núria , Rojo Francisco J. , Pérez-Rigueiro José , Ramos Milagros , Guinea Gustavo V. , Panetsos Fivos , González-Nieto Daniel 
Published: June 2019

AbstractPhysical and cognitive disabilities are hallmarks of a variety of neurological diseases. Stem cell-based therapies are promising solutions to neuroprotect and repair the injured brain and overcome the limited capacity of the central nervous system to recover from damage. It is widely accepted that most benefits of different exogenously transplanted stem cells rely on the secretion of different factors and biomolecules that modulate inflammation, cell death and repair processes in the damaged host tissue. However, few cells survive in cerebral tissue after transplantation, diminishing the therapeutic efficacy. As general rule, cell encapsulation in natural and artificial polymers increases the in vivo engraftment of the transplanted cells. However, we have ignored the consequences of such encapsulation on the secretory activity of these cells. In this study, we investigated the biological compatibility between silk fibroin hydrogels and stem cells of mesenchymal origin, a cell population that has gained increasing attention and popularity in regenerative medicine. Although the survival of mesenchymal stem cells was not affected inside hydrogels, this biomaterial format caused adhesion and proliferation deficits and impaired secretion of several angiogenic, chemoattractant and neurogenic factors while concurrently potentiating the anti-inflammatory capacity of this cell population through a massive release of TGF-Beta-1. Our results set a milestone for the exploration of engineering polymers to modulate the secretory activity of stem cell-based therapies for neurological disorders.
Human endometriotic lesion expression of the miR-144-3p/miR-451a cluster, its correlation with markers of cell survival and origin of lesion content
Authors: Nothnick Warren B. , Swan Kimberly , Flyckt Rebecca , Falcone Tommaso , Graham Amanda 
Published: June 2019

AbstractEndometriosis is an inflammatory condition in which endometrial tissue grows in ectopic locations. Survival and growth of these ectopic lesions is associated with pain and infertility. MicroRNAs (miRNAs) have been postulated to play a role in the pathophysiology of the disease and we have previously demonstrated expression of miR-451 in human endometriotic lesion tissue. Here we report elevated expression of the miR-144-3p/miR-451a cluster in human endometriotic lesion tissue. Use of an endometriotic epithelial cell line (12Z) in which the miRNA processing enzyme, DROSHA, was knocked down resulted in an enrichment in the primary (pri) form of miR-144-3p but not that of pri-miR-451a. Using an experimental mouse model of endometriosis in which ectopic endometriotic lesions were deficient for both of these miRNAs revealed that miR-451a, but not miR-144-3p may be derived from exogenous sources such as the circulation/erythrocytes. Together, these data suggest that the miR-144-3p/miR-451a cluster is expressed in human endometriotic lesion tissue, the level of expression correlates with survival status of the lesion tissue and that miR-451a, but not miR-144-3p may be derived from exogenous sources such as erythrocytes.
Contrast of 83% in reflection measurements on a single quantum dot
Authors: Lochner Pia , Kurzmann Annika , Schott Rüdiger , Wieck Andreas D. , Ludwig Arne , Lorke Axel , Geller Martin 
Published: June 2019

AbstractWe report on a high optical contrast between the photon emission from a single self-assembled quantum dot (QD) and the back-scattered excitation laser light. In an optimized semiconductor heterostructure with an epitaxially grown gate, an optically-matched layer structure and a distributed Bragg reflector, a record value of 83% is obtained; with tilted laser excitation even 885%. This enables measurements on a single dot without lock-in technique or suppression of the laser background by cross-polarization. These findings open up the possibility to perform simultaneously time-resolved and polarization-dependent resonant optical spectroscopy on a single quantum dot.
Endocrine regulation of MFS2 by branchless controls phosphate excretion and stone formation in Drosophila renal tubules
Authors: Rose Emily , Lee Daniela , Xiao Emily , Zhao Wenzhen , Wee Mark , Cohen Jonathan , Bergwitz Clemens 
Published: June 2019

AbstractHow inorganic phosphate (Pi) homeostasis is regulated in Drosophila is currently unknown. We here identify MFS2 as a key Pi transporter in fly renal (Malpighian) tubules. Consistent with its role in Pi excretion, we found that dietary Pi induces MFS2 expression. This results in the formation of Malpighian calcium-Pi stones, while RNAi-mediated knockdown of MFS2 increases blood (hemolymph) Pi and decreases formation of Malpighian tubule stones in flies cultured on high Pi medium. Conversely, microinjection of adults with the phosphaturic human hormone fibroblast growth factor 23 (FGF23) induces tubule expression of MFS2 and decreases blood Pi. This action of FGF23 is blocked by genetic ablation of MFS2. Furthermore, genetic overexpression of the fly FGF branchless (bnl) in the tubules induces expression of MFS2 and increases Malpighian tubule stones suggesting that bnl is the endogenous phosphaturic hormone in adult flies. Finally, genetic ablation of MFS2 increased fly life span, suggesting that Malpighian tubule stones are a key element whereby high Pi diet reduces fly longevity previously reported by us. In conclusion, MFS2 mediates excretion of Pi in Drosophila, which is as in higher species under the hormonal control of FGF-signaling.
Eliciting Response Bias Within Forced Choice Tests to Detect Random Responders
Authors: Orthey Robin , Vrij Aldert , Meijer Ewout , Leal Sharon , Blank Hartmut 
Published: June 2019

AbstractThe Forced Choice Test (FCT) can be used to detect malingered loss of memory or sensory deficits. In this test, examinees are presented with two stimuli, one correct and one incorrect, in regards to a specific event or a perceptual discrimination task. The task is to select the correct answer alternative, or guess if it is unknown. Genuine impairment is associated with test scores that fall within chance performance. In contrast, malingered impairment is associated with purposeful avoidance of correct information, resulting in below chance performance. However, a substantial proportion of malingerers intentionally randomize their responses, and are missed by the test. Here we examine whether a ‘runs test’ and a ‘within test response ‘bias’ have diagnostic value to detect this intentional randomization. We instructed 73 examinees to malinger red/green blindness and subjected them to a FCT. For half of the examinees we manipulated the ambiguity between answer alternatives over the test trials in order to elicit a response bias. Compared to a sample of 10,000 cases of computer generated genuine performance, the runs test and response bias both detected malingered performance better than chance.
Wavelet-based analysis of ground deformation coupling satellite acquisitions (Sentinel-1, SMOS) and data from shallow and deep wells in Southwestern France
Authors: Burnol André , Aochi Hideo , Raucoules Daniel , Veloso Fernanda M. L. , Koudogbo Fifamè N. , Fumagalli Alfio , Chiquet Pierre , Maisons Christophe 
Published: June 2019

AbstractAcquisitions of the Sentinel-1 satellite are processed and comprehensively analyzed to investigate the ground displacement during a three-year period above a double gas storage site (Lussagnet and Izaute) in Southwestern France. Despite quite low vertical displacements (between 4 and 8 mm) compared to the noise level, the cyclic motion reflects the seasonal variations due to charge and discharge during summer and winter periods, respectively. We can simulate the ground deformation at both storage sites by a simple mechanical model. However, ground movements of low-magnitude may be also induced by natural factors, such as the temperature or the soil moisture. Using a wavelet-based analysis, we show there is a soil expansion in the Lussagnet zone that contrasts both in phase and period with the seasonal deformation and that is linked to the surface soil moisture measured by the SMOS satellite. This other displacement is consistent with the water infiltration in the unsaturated zone followed by the swelling of a clay layer. This work reveals the combination of two different processes driving the ground displacement with the same order of magnitude (about 6 mm), namely the pressure variation of a deep gas reservoir and the swelling/shrinking of the shallow subsurface.
Arterial Spin Labeling and Dynamic Susceptibility Contrast-enhanced MR Imaging for evaluation of arteriovenous shunting and tumor hypoxia in glioblastoma
Authors: Nabavizadeh S. Ali , Akbari Hamed , Ware Jeffrey B. , Nasrallah MacLean , Guiry Samantha , Bagley Stephen J. , Desai Arati , Levy Scott , Sarchiapone Whitney , Prior Timothy , Detre John , Wolf Ronald L. , O’Rourke Donald M. , Brem Steven , Davatzikos Christos 
Published: June 2019

AbstractGlioblastoma (GBM) is the most common primary malignant brain tumor in adults and carries a dismal prognosis. Significant challenges in the care of patients with GBM include marked vascular heterogeneity and arteriovenous (AV) shunting, which results in tumor hypoxia and inadequate delivery of systemic treatments to reach tumor cells. In this study, we investigated the utility of different MR perfusion techniques to detect and quantify arteriovenous (AV) shunting and tumor hypoxia in patients with GBM. Macrovascular shunting was present in 33% of subjects, with the degree of shunting ranging from (37–60%) using arterial spin labeling perfusion. Among the dynamic susceptibility contrast-enhanced perfusion curve features, there were a strong negative correlation between hypoxia score, DSC perfusion curve recovery slope (r = −0.72, P = 0.018) and angle (r = −0.73, P = 0.015). The results of this study support the possibility of using arterial spin labeling and pattern analysis of dynamic susceptibility contrast-enhanced MR Imaging for evaluation of arteriovenous shunting and tumor hypoxia in glioblastoma.
First-principles calculations of the epsilon phase of solid oxygen
Authors: Anh Le The , Wada Masahiro , Fukui Hiroshi , Kawatsu Tsutomu , Iitaka Toshiaki 
Published: June 2019

AbstractThe crystal, electronic and magnetic structures of solid oxygen in the epsilon phase have been investigated using the strongly constrained appropriately normed (SCAN) + rVV10 method and the generalized gradient approximation (GGA) + vdW-D + U method. The spin-polarized SCAN + rVV10 method with an 8-atom primitive unit cell provides lattice parameters consistent with the experimental results over the entire pressure range, including the epsilon-zeta structural phase transition at high pressure, but does not provide accurate values of the intermolecular distances d1 and d2 at low pressure. The agreement between the intermolecular distances and the experimental values is greatly improved when a 16-atom conventional unit cell is used. Therefore, the SCAN + rVV10 method with a 16-atom unit cell can be considered the most suitable model for the epsilon phase of solid oxygen. The spin-polarized SCAN + rVV10 model predicts a magnetic phase at low pressure. Since the lattice parameters of the predicted magnetic structure are consistent with the experimental lattice parameters measured at room temperature, our results may suggest that the epsilon phase is magnetic even at room temperature. The GGA + vdW-D + U (with an ad hoc value of Ueff = 2 eV at low pressure instead of the first-principles value of Ulreff ~ 9 eV) and hybrid functional methods provide similar results to the SCAN + rVV10 method; however, they do not provide reasonable values for the intermolecular distances.
The mediating effect of immune markers on the association between ambient air pollution and adult-onset asthma
Authors: Mostafavi Nahid , Jeong Ayoung , Vlaanderen Jelle , Imboden Medea , Vineis Paolo , Jarvis Debbie , Kogevinas Manolis , Probst-Hensch Nicole , Vermeulen Roel 
Published: June 2019

AbstractWe aim to investigate to what extent a set of immune markers mediate the association between air pollution and adult-onset asthma. We considered long-term exposure to multiple air pollution markers and a panel of 13 immune markers in peripheral blood samples collected from 140 adult cases and 199 controls using a nested-case control design. We tested associations between air pollutants and immune markers and adult-onset asthma using mixed-effects (logistic) regression models, adjusted for confounding variables. In order to evaluate a possible mediating effect of the full set of immune markers, we modelled the relationship between asthma and air pollution with a partial least square path model. We observed a strong positive association of IL-1RA [OR 1.37; 95% CI (1.09, 1.73)] with adult-onset asthma. Univariate models did not yield any association between air pollution and immune markers. However, mediation analyses indicated that 15% of the effect of air pollution on risk of adult-onset asthma was mediated through the immune system when considering all immune markers as a latent variable (path coefficient (β) = 0.09; 95% CI: (−0.02, 0.20)). This effect appeared to be stronger for allergic asthma (22%; β = 0.12; 95% CI: (−0.03, 0.27)) and overweight subjects (27%; β = 0.19; 95% CI: (−0.004, 0.38)). Our results provides supportive evidence for a mediating effect of the immune system in the association between air pollution and adult-onset asthma.