bluuebunny/crossref_metadata_2025_split huggingface dataset:
The total mass of the close, double degenerate (DA+DQ) system NLTT 16249
Authors: Vennes Stéphane , Kawka Adela 
Published: December 2024

ABSTRACT
We revisit the binary and stellar properties of the double-degenerate system NLTT 16249. An analysis of new echelle spectra, supported by a joint study of a DQZ velocity template NLTT 44303, confirms the orbital period and constrains the mass ratio revealing a carbon-polluted DQ white dwarf that is up to ${\approx} 6$ per cent more massive than its hydrogen-rich DA companion. Our new model atmosphere analysis of the DA and DQ components, constrained by an accurate Gaia parallax measurement that places the binary at a distance of 57.8 pc, reveals lower mass and temperature than previously estimated for both components, but with higher carbon and nitrogen abundances in the DQ atmosphere. The two components are nearly coeval and could have been generated following a single common envelope event.
Bgee in 2024: focus on curated single-cell RNA-seq datasets, and query tools
Authors: Bastian Frederic B , Cammarata Alessandro Brandulas , Carsanaro Sara , Detering Harald , Huang Wan-Ting , Joye Sagane , Niknejad Anne , Nyamari Marion , Mendes de Farias Tarcisio , Moretti Sébastien , Tzivanopoulou Marianna , Wollbrett Julien , Robinson-Rechavi Marc 
Published: December 2024

Abstract
Bgee (https://www.bgee.org/) is a database to retrieve and compare gene expression patterns in multiple animal species. Expression data are integrated and made comparable between species thanks to consistent data annotation and processing. In the past years, we have integrated single-cell RNA-sequencing expression data into Bgee through careful curation of public datasets in multiple species. We have fully integrated this new technology along with the wealth of other data existing in Bgee. As a result, Bgee can now provide one definitive answer all the way to the cell resolution about a gene’s expression pattern, comparable between species. We have updated our programmatic access tools to adapt to these changes accordingly. We have introduced a new web interface, providing detailed access to our annotations and expression data. It enables users to retrieve data, e.g. for specific organs, cell types or developmental stages, and leverages ontology reasoning to build powerful queries. Finally, we have expanded our species count from 29 to 52, emphasizing fish species critical for vertebrate genome studies, species of agronomic and veterinary importance and nonhuman primates.
ATAC and SAGA histone acetyltransferase modules facilitate transcription factor binding to nucleosomes independent of their acetylation activity
Authors: Chesnutt Kristin V , Yayli Gizem , Toelzer Christine , Damilot Mylène , Cox Khan , Gautam Gunjan , Berger Imre , Tora László , Poirier Michael G 
Published: December 2024

Abstract
Transcription initiation involves the coordination of multiple events, starting with activators binding specific DNA target sequences, which recruit transcription coactivators to open chromatin and enable binding of general transcription factors and RNA polymerase II to promoters. Two key human transcriptional coactivator complexes, ATAC (ADA-two-A-containing) and SAGA (Spt-Ada-Gcn5 acetyltransferase), containing histone acetyltransferase (HAT) activity, target genomic loci to increase promoter accessibility. To better understand the function of ATAC and SAGA HAT complexes, we used in vitro biochemical and biophysical assays to characterize human ATAC and SAGA HAT module interactions with nucleosomes and how a transcription factor (TF) coordinates these interactions. We found that ATAC and SAGA HAT modules bind nucleosomes with high affinity, independent of their HAT activity and the tested TF. ATAC and SAGA HAT modules directly interact with the VP16 activator domain and this domain enhances acetylation activity of both HAT modules. Surprisingly, ATAC and SAGA HAT modules increase TF binding to its DNA target site within the nucleosome by an order of magnitude independent of histone acetylation. Altogether, our results reveal synergistic coordination between HAT modules and a TF, where ATAC and SAGA HAT modules (i) acetylate histones to open chromatin and (ii) facilitate TF targeting within nucleosomes independently of their acetylation activity.
Open Targets Platform: facilitating therapeutic hypotheses building in drug discovery
Authors: Buniello Annalisa , Suveges Daniel , Cruz-Castillo Carlos , Llinares Manuel Bernal , Cornu Helena , Lopez Irene , Tsukanov Kirill , Roldán-Romero Juan María , Mehta Chintan , Fumis Luca , McNeill Graham , Hayhurst James D , Martinez Osorio Ricardo Esteban , Barkhordari Ehsan , Ferrer Javier , Carmona Miguel , Uniyal Prashant , Falaguera Maria J , Rusina Polina , Smit Ines , Schwartzentruber Jeremy , Alegbe Tobi , Ho Vivien W , Considine Daniel , Ge Xiangyu , Szyszkowski Szymon , Tsepilov Yakov , Ghoussaini Maya , Dunham Ian , Hulcoop David G , McDonagh Ellen M , Ochoa David 
Published: December 2024

Abstract
The Open Targets Platform (https://platform.opentargets.org) is a unique, open-source, publicly-available knowledge base providing data and tooling for systematic drug target identification, annotation, and prioritisation. Since our last report, we have expanded the scope of the Platform through a number of significant enhancements and data updates, with the aim to enable our users to formulate more flexible and impactful therapeutic hypotheses. In this context, we have completely revamped our target–disease associations page with more interactive facets and built-in functionalities to empower users with additional control over their experience using the Platform, and added a new Target Prioritisation view. This enables users to prioritise targets based upon clinical precedence, tractability, doability and safety attributes. We have also implemented a direction of effect assessment for eight sources of target–disease association evidence, showing the effect of genetic variation on the function of a target is associated with risk or protection for a trait to inform on potential mechanisms of modulation suitable for disease treatment. These enhancements and the introduction of new back and front-end technologies to support them have increased the impact and usability of our resource within the drug discovery community.
Dicle University Hospital’s hospital disaster plan and emergency service management: Kahramanmaraş earthquake experience
Authors: Yaman Mahmut , Ülgüt Şilan Göger , Şen Abdullah , Ülgüt Ali Fırat , Belek Sema , Durgun Hasan Mansur 
Published: December 2024

Abstract

Background
Hospitals, being establishments with varying functions and capacities, must have disaster plans that reduce vulnerability, ensure the continuity, and possibly increase the capacity of healthcare services; these measures are crucial for reducing mortality and facilitating the normalization of life after a disaster. In this study, the aim was to analyze the operational process of the disaster management plan at Dicle University Faculty of Medicine Hospital following the earthquake in Kahramanmaraş on 6 February 2023.

Methods
This is a retrospective observational study. The study focused on determining the role of the hospital’s disaster plan in crisis situations, specifically examining the emergency service task distribution and management.

Results
The study included patients aged 1 to 85 years (median 34, mean 36.9 ± 19.0), with 52.5% being female. The median injury severity score was 17 (mean 20.1 ± 19.2). Hospital admission was 65.7%, with nephrology and orthopedics being the most common departments. Higher injury severity scores were significantly associated with mortality (P &lt; .05), and dialysis and surgery rates were significantly higher in deceased patients (P &lt; .05). No significant differences were found in age, gender, or comorbidities between groups.

Conclusion
The earthquake highlighted the importance of disaster preparedness in hospitals for effective patient care, collaboration among disciplines, and resource management. Detailed data on the hospital’s disaster plan and its operational process during the earthquake were provided to underscore its critical role in managing the crisis. Lessons learned will shape future disaster response protocols, stressing continual evaluation and improvement in healthcare disaster readiness.
O013 Sleep and overnight emotional memory in older adults with depression: A high-density EEG investigation
Authors: Riccardiello A , Wassing R , Kao C , Kumfor F , Ireland C , Bartlett D , Blackwell C , Grunstein R , McKinnon A , Naismith S , D’Rozario A 
Published: December 2024

Abstract
Older adults with depression have a high incidence of sleep disturbance which is posited to be mechanistically involved in maladaptive overnight emotional memory consolidation. In older adults (≥ 50 years) with and without depression, we aimed to compare group differences in overnight emotional memory and rapid eye movement (REM) and non-rapid eye movement (NREM) (N2 and N3) sleep disturbance. Secondly, we investigated the relationship between emotional memory consolidation, self-report emotional valence and arousal perception, and sleep disturbance.
Participants underwent overnight PSG with high-density EEG. An emotional memory image task with concurrent subjective emotional arousal and valence rating was completed before and after sleep. REM sleep disturbance was measured by REM sleep duration, global REM gamma and alpha activity and REM EEG arousal index. NREM sleep disturbance was measured by NREM sleep duration, global NREM delta, alpha and sigma power, and NREM EEG arousal index. T-tests and non-parametric tests were used for group comparisons. Linear regressions were used to assess relationships between sleep disturbance and emotional memory.
Twenty-two older adults (Depression: n = 12, Control: n = 10) with a mean age of 63.7 ± 6.5 years completed the study. Older adults with depression demonstrated differences in overnight perception of emotional valence and arousal for negative information, suggesting sleep may be involved in emotion perception. Global delta power in NREM was reduced in older adults with depression, suggestive of homeostatic alterations. However, no robust associations between overnight memory consolidation, emotional valence or arousal and REM or NREM sleep disturbance were observed.
O024 Bidirectional associations between maternal and infant sleep and mental health across the perinatal period
Authors: Astbury L , Crowther M , Pinnington D , Milgrom J , Bei B 
Published: December 2024

Abstract

Introduction
The bidirectional relationship between sleep and mental health in the perinatal periods is still poorly understood, with infant sleep rarely considered in this relationship. This longitudinal study investigated the bidirectional relationships between maternal sleep, infant sleep, and maternal depressive and anxiety symptoms over five years.

Methods
This is a secondary data analysis of a randomised controlled trial on maternal sleep health. A community sample of nulliparous participants (N=163, Mage=33.47, SDage=3.52) self-reported depressive and anxiety symptoms, maternal sleep, and infant sleep collected via questionnaires at 30- and 35 weeks’ gestation, 1.5, 3, 6, 12 and 24 months postpartum. Data were analysed using cross-lagged panel analyses, controlling for intervention condition in the larger randomised controlled trial.

Results
Variables were generally associated with each other at the same timepoint and the same variable tended to predict itself at the prospective timepoint. Depressive symptoms at 30 weeks’ gestation predicted maternal insomnia symptoms at 35 weeks’ gestation. Infant night-time sleep duration at 1.5m predicted maternal anxiety at 3m postpartum. Maternal insomnia scores at 1.5m, 6m and 12m predicted maternal anxiety at 3m, 12m, and 24m respectively. Depression symptoms at 12m postpartum predicted maternal insomnia symptoms at 24m postpartum.

Discussion
There are bidirectional associations between maternal, infant sleep and maternal mental health, and the direction of these associations differed between perinatal stages. Future studies are needed to better understand these relationships and examine whether intervening on sleep or mental health may be protective of the other.
O046 Autonomic Control of Heart Rate in Growth Hormone Naïve Children with Prader-Willi Syndrome
Authors: Patoglu O , Walter L , Davey M , Nixon G , Edwards B , Horne R 
Published: December 2024

Abstract

Background
Adults with Prader Willi syndrome (PWS) have an increased risk of cardiovascular morbidity and mortality, which may be due to impaired autonomic control. Heart rate variability (HRV) has been used in different populations as a strong and independent predictor of cardiac mortality and diseases. Studies have not assessed cardiovascular control in children with PWS during sleep while accounting for sleep disordered breathing severity which affects HRV.

Methods
Overnight electrocardiogram recordings during routine sleep studies in children with PWS pre-growth hormone treatment (n=48) and typically developing (TD) children matched in age, obstructive and central apnoea hypopnoea index, body mass index and sex were collected. Power spectral analysis determined HRV for total, low frequency (LF, sympathetic and parasympathetic activity), high frequency (HF, parasympathetic activity) power and LF/HF (sympathovagal balance) during N2, N3, REM and total sleep. Linear mixed-effects modelling adjusting for age, and obstructive and central sleep apnoea severity compared HRV parameters between children with PWS and TD children.

Results
Children with PWS had significantly reduced LF power in N2, REM and total sleep when compared to TD children (p&lt;0.05 for all).

Conclusions
The significant reduction in LF power indicates children with PWS have reduced parasympathetic and sympathetic activity, which may increase cardiovascular risk, however longitudinal studies following children into adulthood are required to confirm this.
The role of silent mutations in KRAS-mutant tumors
Authors: Lu Jun , Zhou Chao , Pan Feng , Liu Hongyu , Jiang Haohua , Zhong Hua , Han Baohui 
Published: December 2024

Abstract
Silent mutations within the RAS gene have garnered increasing attention for their potential roles in tumorigenesis and therapeutic strategies. Kirsten-RAS (KRAS) mutations, predominantly oncogenic, are pivotal drivers in various cancers. While extensive research has elucidated the molecular mechanisms and biological consequences of active KRAS mutations, the functional significance of silent mutations remains relatively understudied. This review synthesizes current knowledge on KRAS silent mutations, highlighting their impact on cancer development. Silent mutations, which do not alter protein sequences but can affect RNA stability and translational efficiency, pose intriguing questions regarding their contribution to tumor biology. Understanding these mutations is crucial for comprehensively unraveling KRAS-driven oncogenesis and exploring novel therapeutic avenues. Moreover, investigations into the clinical implications of silent mutations in KRAS-mutant tumors suggest potential diagnostic and therapeutic strategies. Despite being in early stages, research on KRAS silent mutations holds promise for uncovering novel insights that could inform personalized cancer treatments. In conclusion, this review underscores the evolving landscape of KRAS silent mutations, advocating for further exploration to bridge fundamental biology with clinical applications in oncology.
Chalazia: A Scoping Review to Identify the Evidence Behind Treatments
Authors: Park James K. , Vyas Charu , Dagi Glass Lora R. 
Published: December 2024

Purpose:
Current treatment approaches aimed at resolving a chalazion vary greatly among practice settings and practitioners. Our objective is to provide a review of existing literature on treatment modalities aimed at resolving a chalazion.

Methods:
We conducted a review of existing peer-reviewed publications that described treatment methods aimed at resolving chalazia in patients of any age. Literature searches were conducted using PubMed, Embase, and Cochrane. A 3-stage review process was conducted by 2 trained reviewers, including an initial screening, a full-text review, and data extraction. Articles were assessed for baseline study identifiers, study design, country of origin, number of patients, demographic characteristics, types and number of treatments administered, and resolution rates. Publications were graded using the Oxford Centre for Evidence-Based Medicine levels of evidence table.

Results:
A total of 2814 articles were initially identified based on database queries; 39 studies met inclusion criteria for full-text review. The most represented study design was case series (15), followed by randomized controlled trials (14). Twenty-five publications discussed intralesional corticosteroid injections, 18 discussed incision or excision and curettage, 5 discussed conservative treatments, and 8 discussed topical antibiotic±steroid treatments. Both the definition of chalazion resolution and resolution rates for each treatment modality varied widely between publications.

Conclusions:
Procedural management of chalazia, including corticosteroid injections and surgical management, is most often studied in the literature. Fewer publications exist on the efficacy of conservative and topical treatments.
Differences in Nurse Practitioner Work Effectiveness by Level of Education and Work Experience
Authors: Hampton Debra , Melander Sheila , Rayens Mary Kay 
Published: December 2024

OBJECTIVE
The purpose of this study was to assess the association of educational level, work experience, and other demographic factors with the perceived impact of nurse practitioner role effectiveness.

BACKGROUND
Nurse practitioners (NPs) bring value by impacting patient and organizational outcomes in multiple ways. Employers report difficulties differentiating between how MSN- versus DNP-educated NPs affect patient and organizational outcomes.

METHODS
A cross-sectional descriptive survey design was used in this study that included demographic variables, productivity information, and variables related to perceived organizational outcome impacts.

RESULTS
Participants included 128 NPs with an average of 9.5 years of experience as an NP. Years of practice as an NP was correlated with age. Educational preparation (MSN or DNP) was significantly correlated with overall perceived organizational outcomes and with specific outcomes.

CONCLUSIONS
Based on the results of this study, hiring DNP-prepared NPs is expected to bring a return-on-investment value for organizations.
Assessing alexithymia in chronic pain: psychometric properties of the Toronto Alexithymia Scale-20 and Perth Alexithymia Questionnaire
Authors: Aaron Rachel V. , Preece David A. , Heathcote Lauren C. , Wegener Stephen T. , Campbell Claudia M. , Mun Chung Jung 
Published: December 2024

Abstract

Introduction:
Alexithymia is elevated in chronic pain and relates to poor pain-related outcomes. However, despite concerns from other clinical populations, the psychometric properties of alexithymia measures have not been rigorously established in chronic pain.

Objective:
This study examined the psychometric properties of the Toronto Alexithymia Scale-20 Item (TAS-20) and the Perth Alexithymia Questionnaire (PAQ) in adults with chronic pain.

Methods:
An online sample of adults with chronic pain across the United States (N = 1453) completed the TAS-20, PAQ, and related questionnaires at baseline, 3-month follow-up, and 12-month follow-up.

Results:
Both measures showed good temporal stability, convergent validity (with emotion regulation scores), divergent validity (with depression and anxiety scores), and criterion validity. Some concerns were raised about the TAS-20: the original 3-factor structure showed a poor model fit; the Externally Oriented Thinking subscale of the TAS-20 had poor factor loadings and unacceptable internal consistency; and, we identified several TAS-20 items that may slightly inflate the predictive validity of the TAS-20 on pain-related outcomes. The original 5-factor structure of the PAQ showed a good fit; each PAQ subscale had good factor loadings and excellent internal consistency.

Conclusions:
Both the TAS-20 and PAQ had psychometric strengths. Our data raised some concern for the use of TAS-20 subscales; the PAQ may be a psychometrically stronger option, particularly for investigators interested in alexithymia subscale analysis in people with chronic pain.
Dopamine dynamics in chronic pain: music-induced, sex-dependent, behavioral effects in mice
Authors: Flores-García Montse , Flores África , Aso Ester , Otero-López Paloma , Ciruela Francisco , Videla Sebastià , Grau-Sánchez Jennifer , Rodríguez-Fornells Antoni , Bonaventura Jordi , Fernández-Dueñas Víctor 
Published: December 2024

Abstract

Introduction:
Chronic pain is a debilitating disease that is usually comorbid to anxiety and depression. Current treatment approaches mainly rely on analgesics but often neglect emotional aspects. Nonpharmacological interventions, such as listening to music, have been incorporated into clinics to provide a more comprehensive management of chronic pain. However, the underlying mechanisms of music-mediated pain relief are not fully understood.

Objectives:
Our aim was to evaluate the effects and mechanisms of music exposure in an animal model of chronic pain.

Methods:
We injected mice with the complete Freund adjuvant (CFA) inflammatory agent into the hind paw and housed them for 14 days with background music, or ambient noise, during their active period (Mozart K.205, overnight). The effect of music exposure on nociception, anxiety-like behaviors, and depression-like behaviors was evaluated through different paradigms, including the hot plate, Von Frey, elevated plus maze, splash, and tail suspension tests. In addition, we conducted fiber photometry experiments to investigate whether music influences dopamine dynamics in the nucleus accumbens (NAcc), a crucial region involved in pain processing, anhedonia, and reward.

Results:
Our findings indicate that music exposure prevents the decrease in NAcc activity observed in CFA-injected mice, linking with a sex-dependent reduction in allodynia, anxiety-like behaviors, and depression-like behaviors. Accordingly, female mice were more sensitive to music exposure than male mice.

Conclusion:
Collectively, our findings provide compelling evidence for the integration of music as a nonpharmacological intervention in chronic pain conditions. Moreover, the observed effect on NAcc suggests its potential as a therapeutic target for addressing chronic pain and its associated symptoms.