bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tumor infiltrating lymphocytes can help to identify CD8+ tumor infiltrating lymphocytes and histopathologic subtypes of ovarian carcinoma
Authors: Oktari Desyani , Maulani Heni , Auline Rusminan Suly , Bahar Erial 
Published: July 2019

Abstract
Ovarian carcinoma is the common cause of most death cases in female genital malignancy. Ovarian carcinoma is considered as one of the carcinomas that can evoke immune responses. One of the immune responses is characterized by lymphocyte infiltration of a tumor (tumor infiltrating lymphocytes). This study aims to determine the association of density of tumor infiltrating lymphocytes (TILs) with CD8+ tumor infiltrating lymphocytes and histopathologic subtypes of ovarian carcinoma. This study was a cross-sectional study. Samples were taken based on five main histopathologic subtypes of ovarian carcinoma from January 1, 2015, until December 31, 2017, comprising 50 cases. All preparations were stained with CD8 monoclonal antibody. The density of intratumoral and stromal TILs was subsequently evaluated on histopathologic preparations and immunohistochemical examination. The association between the density of TILs, CD8+ tumor-infiltrating lymphocytes and histopathologic subtypes of ovarian carcinoma was evaluated using Spearman, Kruskal-Wallis, and Mann-Whitney test. The density of TILs and intratumoral CD8+ TILs were associated with histopathologic subtypes, especially high-grade serous carcinoma that had a different density value compared to other histopathologic subtypes. In addition, the density of TIL and intratumoral CD8+ TIL had a meaningful association with histopathologic subtypes of ovarian carcinoma.
Seluang (Rasbora agrotyenia) Fish Oil Increases Vitamin D in Autoimmune Patients (Systemic Lupus Erythematosus)
Authors: Gonzales Penserga Ester , Umi Partan Radiyati , Hidayat Rachmat , Saputra Nurwan , Prapto Hari , Rahmayani Febri 
Published: July 2019

Abstract
Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease which usually affects multiple organ systems. Its patients are prone to hypovitaminosis D along with its complications such as osteoporosis. The improvement of vitamin D status in SLE patients is associated with better immune modulation, proinflammatory cytokine suppression, calcium absorption improvement, as well as better severity and lower osteoclastogenesis. This double-blind, randomised clinical trial was conducted in the Allergology-Immunology Polyclinic in the Internal Medicine Department of Mohammad Hoesin General Hospital, Palembang, Indonesia, over the period April 2017 – February 2018. Samples were given either seluang (Rasbora agrotyenia) fish oil or placebo for 12 weeks. On the start and the end of the study, vitamin D levels were examined. 32 patients, all female, were divided evenly into the two groups. On the end of the study, the seluang oil group had significant vitamin D increase compared to placebo (p = 0.000). We concluded that the administration of seluang fish oil was able to increase vitamin D levels in SLE patients.
CD8+ Serum as a predictor of Neoadjuvant Chemotherapy Response in Locally Advanced Breast Cancer
Authors: Qodir Nur , Umar Mulawan , Darwin Nopriansyah , Theodorus  
Published: July 2019

Abstract
CD8+ Cytotoxic T-cell Lymphocytes (CTLs) had a major role in tumour apoptosis. Meanwhile, chemotherapy could induce tumour cell apoptosis and enhance CTL response. Therefore, pre-treatment immune status might predict the ability of chemotherapy to eliminate cancer cells. The aim of this study was to evaluate the efficacy of neoadjuvant chemotherapy to CD8+ serum in locally advanced breast cancer patient. A randomized clinical trial has been conducted in Dr Mohammad Hoesin Palembang from October 2017 until January 2018. There were 30 samples who fulfil the inclusion criteria. All samples were taken neoadjuvant chemotherapy and analysis data was using SPSS ver.21. The range of age patient with locally advanced breast cancer in this study 30-66 y.o with mean 45±10.526 y.o. After neoadjuvant chemotherapy, the CD8+ serum was significantly decreased (p=0.000). CD8+ serum before chemotherapy has sensitivity 42.86% and specificity 43.48% to predict neoadjuvant chemotherapy response with cut off point 660.7 cell/mm3. Neoadjuvant chemotherapy decreases CD8+ significantly in locally advanced breast cancer patient.
Factors of soil-transmitted helminths infections in children who live in the surrounding of the final disposal landfill of Sukawinatan, Palembang
Authors: Ramayanti Indri , Ghiffari Ahmad 
Published: July 2019

Abstract
Soil-Transmitted Helminths (STH) is a group of nematodes that infect people and transmitted through soil media. STH occurs especially among pre-school and school-aged children, and commonly related to environmental sanitation and personal hygiene. The study objected to determine the factors related to the incidence of STH in children 5-15 years who lived surrounding the Sukawinatan district of Palembang city. The observational analytic using the cross-sectional design, consisted of 110 subjects sampled by consecutive sampling. Data on environmental sanitation and personal hygiene were obtained by questionnaires, while infection status using the Kato-Katz faecal technic. The results were analyzed using Chi-square test (α = 0.05), showed that 24.5% of population where infected with STH. A number of 1-24-2 children were infected with hookworm-Ascaris lumbricoides-Trichuris trichiura infection, respectively. Based on statistical test results, the association of STH infection with variables were: waste disposal (p = 0.268), water facilities (p = 1.000), sewage disposal (p = 0.224), latrine (p = 0.021), hand washing prior to meal (p = 0.001), hand washing after defecate (p = 0.028), use of footwear (p = 0.013), and nail hygiene (p = 1.000). Concluded that the significant factors related to STH were use of latrine, hand washing behaviour, and use of footwear. Further research will be necessary to successfully eliminate this neglected tropical disease.
A preliminary study: Centella asiatica extract modulates acetylcholine in the heart
Authors: Augusta Rosdah Ayeshah , Lusiana Evi , Reagan Muhammad , Akib Abdurrahman , Khairunnisa Fadhila , Husna Afkara 
Published: July 2019

Abstract
Previous studies have demonstrated the cardioprotective effect of acetylcholine (Ach), and therefore is indeed a potential theraupetic target for cardiovascular disease. Recent studies have shown that Ach is not only important in the pathological but also physiological state of the heart. The herb Centella asiatica has long been used to cure a number of conditions in traditional Indian medicine. Previous studies have shown that the acetylcholinesterase inhibitory activity of the Centella asiatica extract is capable of increasing the Ach level in the brain. However, it is unclear whether it can affect Ach level in other organs, such as the heart. In this study, healthy adult male Wistar rats were allocated into groups treated with the positive control, Donepezil, or the aqueous extract at doses 200 mg/kg, 400 mg/kg, and 800 mg/kg. After 21 days, the whole heart was evacuated and weighed prior to further measurement for Ach content. The present study showed that long-term consumption of 200 mg/kg and 400 mg/kg of aqueous Centella asiatica extract could modulate the Ach content in the heart.
Cutaneous larva migrans - a case report in amplas community health centre, Medan
Authors: Sakina Adriani , Darlan Dewi 
Published: July 2019

Abstract
Cutaneous Larva Migrans (CLM) is one of the most helminthic skin infestations, frequently in tropics and subtropics countries, including Southeastern United States, Central, and South America, Southeast Asia, and Africa. The infected cats and dogs usually defecate in sandpits, seashore, or areas with loose, wet soils where also being the natural environments for the larvae. Human is the dead-end hosts that the filariform larvae in humans are not capable of maturation. The larvae keep moving approximately 2 – 3 cm/day. During its migration, an itchy erythematous. CLM is a self-limiting disease, the spontaneous resolution coming up within a few weeks. But most patients used to receive anti-parasite therapy, such as Albendazole or Ivermectin.
Identification of KRAS gene codon 12 polymorphism in colorectal cancer patients at Mohammad Hoesin General Hospital Palembang
Authors: Saleh Irsan , Triwani  , Hayati Lusia , Surya Fatimah Liliana , Subandrate  
Published: July 2019

Abstract
Colorectal cancer occurs due to neoplastic transformation of epithelial cells in the colon and rectum resulting from the accumulation of genetic and epigenetic aberrations. One of the genetic factors associated with colorectal cancer is KRAS gene polymorphism, which mostly occurs in codon 12. KRAS gene polymorphism leads to constitutional activation of KRAS protein effectors which will initiate signalling network to nucleus to increase cell proliferation, survival and differentiation. The aim of this study was to identify the KRAS gene codon 12 polymorphism in colorectal cancer patients at Mohammad Hoesin General Hospital Palembang. This study was a descriptive laboratory study with PCR–RFLP technique using BstNI enzyme on thirty patients with colorectal cancer. Wild type genotype (GG) of KRAS gene codon 12 found in 15 patients (50%), heterozygous mutant genotype (Gg) found in 12 patients (40%) and homozygous mutant genotype (gg) only found in 3 patients (10%). The G allele frequency was 70% and the g was 30%. The most common genotype found in colorectal cancer patients was GG genotype and most common alotype was G allele. KRAS gene polymorphism was found in half of the colorectal cancer patients, heterozygous mutant genotype (Gg) was more frequent then homozygous mutant genotype (gg).
Histoplasmosis of bilateral adrenal masses and submandibular lymphadenopathy: other diagnosis besides malignancy and tuberculosis
Authors: Aprilia Salim Nelda , Tanzil Iswadi , Permata Mega , Hudari Harun , Perdana Rizky 
Published: July 2019

Abstract
Histoplasma capsulatum is a dimorphic fungus which can cause infection usually in immunocompromised patients. An immunocompetent patient can be affected if he got chronic exposure to soil contaminated with birds or bats guano. We are reporting a case of a 54 years old man, working as trashman at open-air landfill, presented to hospital, with persistent fever and abdominal pain. The patient also complained of enlargement of submandibular lymph nodes. The abdominal ultrasound and CT-scan showed bilateral adrenal masses. Biopsy per laparoscopy of adrenal mass, and aspiration cytology of lymphadenopathy were performed and the results of cyto and histopathology examinations were Histoplasma capsulatum infection. The patient was planned for itraconazole 200 mg bid for 12-24 months. After 6 months of therapy, the patient had no more complains and the lymph nodes have shrinked significantly.
Supplementation of curcuma domestica extract reduces cox-2 and inos expression on raw 264.7 cells
Authors: Setyono Joko , Murti Harini Ika , Sarmoko Sarmoko , Rujito Lantip 
Published: July 2019

Abstract
The expression of COX-2 and iNOS have an important role in inflammation. Turmeric (Curcuma domestica) contains active compounds such as curcumin, which can inhibit the expression of the enzyme so that the risk of chronic inflammation and cancer can be reduced. The aim of this study was to determine the anti-inflammatory effects of turmeric extract concentrations on the expression of COX-2 and iNOS in RAW 264.7 cells induced by lipopolysaccharide (LPS). This study was an experimental post-test only with a control group design. The RAW 264.7 cultured cells were divided into six groups (G) on a 24-well plate; GA (control group without LPS), GB (control group with LPS), GC (control group without primary antibody), GD (62,5μg/mL extract concentration), GE (125μg/mL extract concentration), and GF (250μg/mL extract concentration). After 18 hours of treatment, all the cells were fixed with ethanol and stored and tested with immunocytochemistry. Results show the expressions of COX-2 and iNOS in GA (10,51±5,15; 12,51±3.10), GB (82,29±1,49; 82,70±1,67), GC (29,01±5,19; 16,33±1,61), GD (32,19±5,36; 58,58±10,31), GE (24,29±5,88; 52,55±9,03), and GF (40,42±3,15; 29,24±7,84). The test results of data analysis by one-way ANOVA (COX-2) and Kruskal-Wallis (iNOS) showed significant differences (p <0.05) along with Post Hoc test Tamhanes (COX-2) and Mann-Whitney (iNOS), which also showed significant differences (p <0.05). The most substantial differences shown by the mean of the treatment group turmeric extract concentration were 125 μg/mL (COX-2) and 250μg/mL (iNOS). These results suggest that turmeric extracts can reduce the effects of inflammation on RAW 264.7 cells induced by lipopolysaccharide
Factors influencing the accuracy of ETT size prediction in children with CHD: a pilot study
Authors: Soenarto R F , Kusuma H 
Published: July 2019

Abstract
Induction and intubation for pediatric patients with congenital heart defects (CHD) should be conducted with careful measures to avoid any complications due to anesthetic drugs. Proper endotracheal tube (ETT) size is essential to avoid reintubation. The aim of this study is to analyze factors affecting the accuracy of uncuffed ETT size prediction in paediatrics with CHD. This observational-analytic study included seventeen patients aged 0-8 years with CHD underwent general anesthesia with tracheal intubation. Subjects were classified as cyanotic or acyanotic heart disease. The ETT size was predicted by Cole’s formula. The accuracy of prediction was analyzed according to age, body weight, height and type of CHD. The prediction of ETT size was accurate in 53% of the subjects. The prediction was inaccurate in 63% of cyanotic subjects, while only 16.7% of acyanotic subjects. Univariate analysis for all variables showed there was no significant factor to affect the accuracy of ETT size prediction. The smallest p value was subject’s height. Height has a 69.8% affecting factor to predict the accurate ETT size. Thus, age, body weight, height and type of CHD are not statistically significant factors to affect the accuracy of ETT size prediction. But, height gives a better prediction to the accuracy of ETT size.
Correlation between flat feet and body mass index in primary school students
Authors: Suciati Tri , Rulan Adnindya Msy , Seta Septadina Indri , Putri Pratiwi Poppy 
Published: July 2019

Abstract
Body mass index is one of the indicators used to detect body weight and nutritional status. Excessive weight in children can affect the occurrence of flat feet compared with children who have normal weight. Flat feet is a foot structure disorder that does not form the medial arch. The aim of this research is to know the relationship between body mass index and flat feet in elementary school children at Xaverius 1 Palembang. This is an analytical-observational research with a cross-sectional design. The subjects of this research are 70 students of elementary school at Xaverius 1 Palembang and collected by using stratified random sampling proportionally. The research was conducted in October 2017. Data analysis was performed by Chi-Square test on SPSS application. The category of body mass index was classified into overweight/obese and not-overweight. In the category of overweight/obese, there are 24 (80%) people were positive flat feet and 6 (20%) normal people. In the category of not-overweight, there are 7 (17.5%) people were positive flat feet and 33 (82.5%) normal people. The result of the bivariate analysis showed that there was a significant relationship between body mass index and flat feet (p = 0,000) with PR value of 4.571, that is overweight/obese people risk 4.5 times for flat feet compared to people who were notoverweight.
Antidepressant activity of basil leaves essential oil (Ocimum basilicum) in male balb/c mice
Authors: Rizki Suryani Puji , Febri Zulissetiana Eka , Arroyantri Prananjaya Bintang 
Published: July 2019

Abstract
Depression is the most common mental disorder nowadays. It was triggered many searches to find out traditional plants that has antidepressant activity, one of which is sweet basil. Sweet basil oil that consist of eugenol inspected by in vivo using depression-induced male Balb/c mice and compared its antidepressant activity with fluoxetine HCl. This study was to compare antidepressant activity of sweet basil oil (Ocimum basilicum) and fluoxetine HCl evaluated from immobility time and serotonin level in plasma. An experimental study, in vivo, has been done in animal house and biomolecular laboratory medical faculty of Sriwijaya university during May until July 2015. Samples of 32 male Balb/c mice that divided into 4 groups was adapted for 10 days. Tail suspension test was done for 14 days, then mice blood was taken for checking depression serotonin level. In day 15th until 28th, mice given with sweet basil oil with gradually dose (P1, P2, and P3) and fluoxetine HCl (P4). Tail suspension test was done once and mice blood was taken for checking after therapy serotonin level. Data analyzed with SPSS 16. All male mice are similar in body weight, immobility time at tail suspension test I, and depression serotonin level. By tail suspension test, there is significant difference before and after therapy in group P1, P2, and P3 (p <0,001), group P4 (p = 0,001). There is no dose suitability between sweet basil oil and fluoxetine HCl based on tail suspension test (p < 0,001). Serotonin level in plasma before and after therapy shows significant difference in all groups (p < 0,001). There is no dose suitability between sweet basil oil and fluoxetine HCl based on serotonin level in plasma (p < 0,001). Antidepressant activity of sweet basil oil and fluoxetine HCl is not similar based on immobility time and serotonin level in plasma.
Experimental Investigation of Recycled Machining Coolant Mixed with Nanofluids Based Al2O3
Authors: Lim S K , Mohamad N Z , Yusoff A R 
Published: July 2019

Abstract
Machining coolant is used in manufacturing industry for lubrication, rusting control and cooling which are crucial in operations such as grinding and cutting process. However, the machining coolant lose efficiency in months due to the thermal degradation and contamination. To recycle the used of machining coolant while enhancing the performance, the mixture of nanofluids (N) based Al2O3 with better thermal properties to synthesize with the recycled machining coolant (RMC). This study suspended Al2O3 nanofluids in four base ratios of recycled machining coolant (i.e. 0:100, 20:80, 40:60 and 60:40) by ultrasonic homogenies. The three main parameters in cooling rate performance and rusting control are thermal conductivity, dynamic viscosity and pH indication, respectively. The thermal conductivity and dynamic viscosity are then measured at temperature range of 30 to 60°C. The highest enhancement of thermal conductivity was evaluated to be 37.9% higher than machining coolant base fluid at the temperature of 60°C in 60:40 (N:RMC). However, the enhancement of dynamic viscosity was measured to be 4.5% for 60:40 (N:RMC) at 60°C. The pH value of 60:40 (N:RMC) at ambient temperature obtained 8.18 value was nearly neutral and decreased the rusting process. Therefore, this study recommends the use of recycled machining coolants based Al2O3 as cutting fluid in the ratio of 60:40 for application in machining operations.
Energy Efficiency Assessment in Production Line: An Approach towards Sustainable Manufacturing
Authors: Saleh C , Prasetiyo R , Hendradewa A P , Hassan A B 
Published: July 2019

Abstract
Global demands of energy which causes a quick depletion of the natural energy reserves become a critical concern. Industrial sector is concerned to be the largest energy consumer compared to other sectors. Thus, an energy efficiency improvement tends to be a proper solution in addressing this problem. This work aims to analyse a prospect energy efficiency improvement within a production line. A discrete-event simulation (DES) was used to investigate the energy saving potential and a case study was conducted in a crumb rubber company. Three parameters were taken into consideration to calculate the energy consumption, i.e. processing time, working shift schedule, and machine states. An initial model which represents an actual condition of the manufacturing production line was developed. From the analysis of the initial model result, a recommendation scenario was built. It was shown that a reduction of 8.22% in the energy consumption is achievable by implementing the suggested scenario.
An Application of Cutting-Stock Problem in Green Manufacturing: A Case Study of Wooden Pallet Industry
Authors: Wattanasiriseth P , Krairit A 
Published: July 2019

Abstract
The cutting-stock problem is used to minimize the impact of wasting material during the stock cutting process. The linear programming model is developed and implemented on Microsoft Excel to solve for the optimal cutting plan with the criteria of minimizing material waste. The case study of pine wood stock cutting in wooden pallet industry demonstrates the benefit of using the approach in terms of reducing production cost and carbon emission. The results show that waste can be reduced up to 70.86% resulting in a cost reduction of 977,352 Baht/year. The amount of carbon dioxide equivalent emitted from the life cycle of pine wood is reduced by 22,713 kg CO2-e/year or approximately 5.32% reduction.
Chipping Detection in Ceramic Insert during Turning by Analysis of Workpiece Surface Profile Using Cross-Correlation
Authors: Mohamad N A , Ratnam M M 
Published: July 2019

Abstract
Chipping in ceramic cutting inserts during machining can cause detrimental effects on the surface finish quality of the workpiece. Current methods of detecting chipping in ceramic inserts do not compare the minute changes in the workpiece profile in subsequent machining passes. In this work, an effective method of detecting chipping in ceramic inserts by analysing the workpiece surface profile using cross-correlation is proposed. In this method, the sub-pixel edge location method was used to extract the surface profile captured using a digital camera. Surface profiles corresponding to subsequent machining passes were cross-correlated at three different sections of the workpiece. An abrupt drop in the correlation coefficient was observed in the middle and end sections of the workpiece after the fifth machining pass, thus providing a reliable measure of tool chipping.
Effect of Machining Parameters on the Surface Roughness for Different Type of Materials
Authors: Ghazali M H M , Mazlan A Z A , Wei L M , Tying C T , Sze T S , Jamil N I M 
Published: July 2019

Abstract
Surface roughness (Ra
) is an important parameter in determining the quality of surface finishing of any products obtained from the machining process. Surface roughness can be affected by several factors such as machining parameters, workpiece material properties, cutting tool parameters and machine tool conditions. In this study, the effects of machining parameters such as feed rate and cutting speed on the surface roughness was investigated for aluminium, mild steel and brass materials during the turning process using CNC Lathe machine. The experimental Ra values obtained were compared with the theoretical values with discrepancy ranging from 1.14 % to 113.71 %. From the study, it can be concluded that the surface roughness of materials will increases as the feed rate increases and decrease when higher cutting speed is applied.