bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolution-guided engineering of small-molecule biosensors
Authors: Snoek Tim , Chaberski Evan K. , Ambri Francesca , Kol Stefan , Bjørn Sara P. , Pang Bo , Barajas Jesus F. , Welner Ditte H. , Jensen Michael K. , Keasling Jay D. 
Published: April 2019

AbstractAllosteric transcription factors (aTFs) have proven widely applicable for biotechnology and synthetic biology as ligand-specific biosensors enabling real-time monitoring, selection and regulation of cellular metabolism. However, both the biosensor specificity and the correlation between ligand concentration and biosensor output signal, also known as the transfer function, often needs to be optimized before meeting application needs. Here, we present a versatile and high-throughput method to evolve and functionalize prokaryotic aTF specificity and transfer functions in a eukaryote chassis, namely baker’s yeastSaccharomyces cerevisiae. From a single round of directed evolution of the effector-binding domain (EBD) coupled with various toggled selection regimes, we robustly select aTF variants of thecis, cis-muconic acid-inducible transcription factor BenM evolved for change in ligand specificity, increased dynamic output range, shifts in operational range, and a complete inversion of function from activation to repression. Importantly, by targeting only the EBD, the evolved biosensors display DNA-binding affinities similar to BenM, and are functional when ported back into a non-native prokaryote chassis. The developed platform technology thus leverages aTF evolvability for the development of new host-agnostic biosensors with user-defined small-molecule specificities and transfer functions.
A novel tool for monitoring endogenous human MYC transcription and translation by EGFP tag insertion at the 3’ end using CRISPR-Cas9 genome editing
Authors: Cumbal N. , Cole MD. 
Published: April 2019

AbstractThe MYC oncogene is overexpressed in over 70% of human cancers. Since its identification, the study of MYC has led to the discovery of the various ways through which oncogenes contribute to the ability of normal cells to become malignant. However, there are many aspects of MYC biology that remain unknown or controversial in terms of its regulation, targetability and downstream control of its targets. We developed two stable cell lines expressing MYC endogenously tagged with EGFP via CRISPR/Cas9-mediated genome editing. This system allows efficient detection of transcriptional activity of MYC as well the resulting fusion protein while maintaining the gene expression profiles, growth factors-associated MYC induction and growth kinetics of the parental cells. To our knowledge, this is the first report showing endogenous monitoring of MYC expression in colorectal adenocarcinoma through an EGFP tag, thus making it an efficient tool for high-throughput approaches such as genetic and drug screens.
Effect of nanofluids on heat transfer and cooling system of the photovoltaic/thermal performance
Authors: Nasrin Rehena , Hasanuzzaman Md. , Rahim N.A. 
Published: April 2019

Purpose
Effective cooling is one of the challenges for photovoltaic thermal (PVT) systems to maintain the PV operating temperature. One of the best ways to enhance rate of heat transfer of the PVT system is using advanced working fluids such as nanofluids. The purpose of this research is to develop a numerical model for designing different form of thermal collector systems with different materials. It is concluded that PVT system operated by nanofluid is more effective than water-based PVT system.

Design/methodology/approach
In this research, a three-dimensional numerical model of PVT with new baffle-based thermal collector system has been developed and solved using finite element method-based COMSOL Multyphysics software. Water-based different nanofluids (Ag, Cu, Al, etc.), various solid volume fractions up to 3 per cent and variation of inlet temperature (20-40Â°C) have been applied to obtain high thermal efficiency of this system.

Findings
The numerical results show that increasing solid volume fraction increases the thermal performance of PVT system operated by nanofluids, and optimum solid concentration is 2 per cent. The thermal efficiency is enhanced approximately by 7.49, 7.08 and 4.97 per cent for PVT system operated by water/Ag, water/Cu and water/Al nanofluids, respectively, compared to water. The extracted thermal energy from the PVT system decreases by 53.13, 52.69, 42.37 and 38.99 W for water, water/Al, water/Cu and water/Ag nanofluids, respectively, due to each 1°C increase in inlet temperature. The heat transfer rate from heat exchanger to cooling fluid enhances by about 18.43, 27.45 and 31.37 per cent for the PVT system operated by water/Al, water/Cu, water/Ag, respectively, compared to water.

Originality/value
This study is original and is not being considered for publication elsewhere. This is also not currently under review with any other journal.
SLAC – a semi-Lagrangian artificial compressibility solver for steady-state incompressible flows
Authors: Mortezazadeh Mohammad , Wang Liangzhu (Leon) 
Published: April 2019

Purpose
The purpose of this paper is the development of a new density-based (DB) semi-Lagrangian method to speed up the conventional pressure-based (PB) semi-Lagrangian methods.

Design/methodology/approach
The semi-Lagrangian-based solvers are typically PB, i.e. semi-Lagrangian pressure-based (SLPB) solvers, where a Poisson equation is solved for obtaining the pressure field and ensuring a divergence-free flow field. As an elliptic-type equation, the Poisson equation often relies on an iterative solution, so it can create a challenge of parallel computing and a bottleneck of computing speed. This study proposes a new DB semi-Lagrangian method, i.e. the semi-Lagrangian artificial compressibility (SLAC), which replaces the Poisson equation by a hyperbolic continuity equation with an added artificial compressibility (AC) term, so a time-marching solution is possible. Without the Poisson equation, the proposed SLAC solver is faster, particularly for the cases with more computational cells, and better suited for parallel computing.

Findings
The study compares the accuracy and the computing speeds of both SLPB and SLAC solvers for the lid-driven cavity flow and the step-flow problems. It shows that the proposed SLAC solver is able to achieve the same results as the SLPB, whereas with a 3.03 times speed up before using the OpenMP parallelization and a 3.35 times speed up for the large grid number case (512 × 512) after the parallelization. The speed up can be improved further for larger cases because of increasing the condition number of the coefficient matrixes of the Poisson equation.

Originality/value
This paper proposes a method of avoiding solving the Poisson equation, a typical computing bottleneck for semi-Lagrangian-based fluid solvers by converting the conventional PB solver (SLPB) to the DB solver (SLAC) through the addition of the AC term. The method simplifies and facilitates the parallelization process of semi-Lagrangian-based fluid solvers for modern HPC infrastructures, such as OpenMP and GPU computing.
Preservice EFL teachers’ collaborative understanding of lesson study
Authors: Ousseini Hamissou 
Published: April 2019

PurposeThe purpose of this paper is to report on a small-scale research undertaken to examine the preservice English as foreign language (EFL) teachers’ collaborative understanding of lesson study. It constitutes the first of two empirical steps undertaken for integrating lesson study in initial EFL teacher education. The paper takes to the belief that preservice teachers’ understanding is a key factor that determines success or failure of lesson study projects and should therefore be investigated.Design/methodology/approachThere were four preservice teachers who volunteered to participate in the study. Data were collected based on two procedures. Participants read the works of Lewis and Tsuchida (1999) and Dudley (2014) and were asked to collaboratively reflect about their readings on a WhatsApp platform. They were afterwards brought to a face-to-face discussion guided by questions mostly built from their previous reflections on the WhatsApp platform.FindingsParticipants have demonstrated a joint understanding which was essentially achieved due to the collaborative procedures. Likewise, participants have collaboratively reflected on the factors that could affect the process of lesson study and its application to EFL classrooms in Niger. These factors have been related to the issues in research methodology and the contextual learning culture.Originality/valueThe findings from this study draw attention to the need for teacher educators to use interactive and collaborative strategies while instructing preservice teachers about lesson study. The paper also offers insights about contextual factors which require preliminary and immediate actions before implementing lesson study in Niger.
Computational fluid dynamic study on the tribological performance of dimple-textured surface fabricated using the turning process
Authors: Abdul Rahman Haniff , A. Ghani Jaharah , Wan Mahmood Wan Mohd Faizal , Mohammad Rasani Mohammad Rasidi 
Published: April 2019

Purpose
This study aims to simulate the influence of surface texturing produced via turning process toward pressure distribution and load capacity generation using computational fluid dynamics (CFD).

Design/methodology/approach
The dimple geometry was obtained via turning process, to be used for future application on piston skirt surfaces. Two cases were studied: a preliminary study using single periodic dimple assuming linear dimple distribution and an application study using multiple periodic dimples to address actual dimple orientation following the turning process.

Findings
For the first case, the dimple was proven to generate load capacity with regard to untextured surface, owing to the asymmetric pressure distribution. Increasing the Reynolds number, dimple width and dimple depth was found to increase load capacity. For the second case, although load capacity increases via surface texturing, the value was 97.4 per cent lower relative to the first case. This confirmed the importance of doing multiple dimple simulations for real applications to achieve more realistic and accurate results.

Originality/value
A new concept of dimple fabrication using a low-cost turning process has been developed, with a potential to increase the tribological performance under hydrodynamic lubrication. Previous CFD simulations to simulate these benefits have been done using a single periodic dimple, assuming equal distribution array between dimples. However, due to the different orientations present for dimples produced using turning process, a single periodic dimple simulation may not be accurate, and instead, multiple dimple simulation is required. Therefore, present research was conducted to compare the results between these two cases and to ensure the accuracy of CFD simulation for this type of dimple.
Panel report: the dark side of the digitization of the individual
Authors: Turel Ofir , Matt Christian , Trenz Manuel , Cheung Christy M.K. , D’Arcy* John , Qahri-Saremi* Hamed , Tarafdar* Monideepa 
Published: April 2019

Purpose
Digital technologies have diffused into many personal life domains. This has created many new phenomena that require systematic theorizing, testing and understanding. Such phenomena have been studied under the Digitization of the Individual (DOTI) umbrella and have been discussed in the DOTI pre-International Conference on Information Systems workshop for the last three years (from 2015 to 2017). While prior years have focused on a variety of issues, this year (2018) we decided to put special emphasis on negative effects of the DOTI, i.e., “the dark side” of the DOTI.

Design/methodology/approach
This manuscript reports on a panel of three experts (in alphabetical order: John D’Arcy, Hamed Qahri-Saremi and Monideepa Tarafdar) who presented their past research in this domain, as well as their outlook for future research and methodologies in research on the DOTI.

Findings
The authors introduce the topic, chronicle the responses of the panelists to the questions the authors posed, and summarize and discuss their response, such that readers can develop a good idea regarding next steps in research on the dark side of the DOTI.

Originality/value
The authors introduce the topic of the dark sides of DOTI and point readers to promising research directions and methodologies for further exploring this relatively uncharted field of research.
Wanted: strategic partners for young firms eager to enter foreign markets
Authors: Mi Lili , Kang Yuanfei , Liu Yulong 
Published: April 2019

Purpose
This paper aims to investigate the relationship between strategic asset-seeking intent and firms’ entry strategies of foreign investment in the context of emerging market firms.

Design/methodology/approach
This study is based on survey data of 392 Chinese foreign direct investment projects. Structural equation modelling is used for data analysis.

Findings
With stronger strategic asset-seeking intent, emerging market multinational enterprises are likely to locate their subsidiaries in developed countries, use a wholly owned subsidiary mode and invest with greater intensity, while they do not have a clear preference in entry timing.

Practical implications
The strategic asset-seeking intent applies not only to emerging market firms but also to small and medium firms in general that have limited resources and a need to catch up with stronger competitors. This study therefore provides guidance to these firms.

Originality/value
This study contributes by investigating how the strategic asset-seeking intent affects firms’ strategies. The findings have practical implications for strategic managerial decisions that lead to sustained competitive advantage and improved firm performance.
Impact of religiosity on ethical judgement: a study on preference of retail stores among consumers
Authors: Uysal Adem , Okumuş Abdullah 
Published: April 2019

Purpose
The purpose of this study is to identify the impact of ethical judgements, depending on religiosity level of consumers, on decisions of buying or not buying products from supermarkets selling alcoholic beverages.

Design/methodology/approach
The scope of this study covers consumers from province of Mus in Turkey who indicate they go shopping at supermarkets. Accordingly, data are collected from 362 consumers via face-to-face survey and the results are evaluated through regression analysis.

Findings
According to analysis, it was found that the internal and external religiosity of the consumers positively affected their ethical judgement towards the markets selling alcoholic beverages. Besides, it is concluded that the ethical judgements of consumers have a positive impact on boycott decisions against supermarkets selling alcoholic beverages.

Originality/value
The survey contributes to relevant literature by putting forth the impact of ethical judgements of consumers, associated with their level of religiosity, on their decisions as to preference of supermarkets. Moreover, this study is consistent with the previous studies and supports the conclusion that the ethical decisions are affected by the extrinsic and intrinsic levels of religiosity; however, they are more affected by the intrinsic levels of religiosity.
How to improve innovation in sustainable tourism? Five lessons learned from the Austrian Alps
Authors: Heslinga Jasper Hessel , Hillebrand Hans , Emonts Tanja 
Published: April 2019

Purpose
The purpose of this paper is to propose lessons learned on how innovation can help facilitate sustainable tourism in regions. Sustainable tourism is a promising concept for a resilient future of tourism destinations, however, its practical implication remains a challenge.

Design/methodology/approach
To gain these practical lessons on innovation, a so-called “inspirational journey” was organized. An interdisciplinary group of experts went to the Austrian Alps and many local initiatives and innovative ideas were presented.

Findings
As a result of this journey, the authors advocate five lessons that can help improve sustainable tourism in the future.

Practical implications
Being a source of inspiration for policy-makers and planners, these lessons can be applied to other regions.

Originality/value
The added value of this viewpoint paper is that it provides practical examples on how innovation can help improve sustainable tourism in the future.
Are all observations measurements?
Authors: Kadri Faisal L. 
Published: April 2019

Purpose
The purpose of this study is to investigate the possibility of soft science measurement of motivation under strict hard science criteria from observations of individual animals and to suggest the conditions under which an observation can be classified as a measurement.

Design/methodology/approach
The methodology starts from reconciling second-order cybernetics/radical constructivism (SOC/RC) understanding of the central role of the observer with physical measurements, which accepts the existence of a mind-independent reality. As a result of the reconciliation, parallels were identified between the SOC/RC experiences of as_is and as_if, on the one hand, and the measurement concepts of accuracy and resolution, on the other hand. The scales of physical measurement are defined by criteria of varying strictness, and the scales that meet the strict criterion of concatenation are generally considered hard science and lead to well-defined accuracy and precision. The similarity between SOC/RC and physical measurement suggests that if accuracy and precision can be computed from observations, then the observations can be classified as measurements in a strict hard science fashion; otherwise, the observations are just observations.

Findings
A nonlinear dynamic model of motivation is reintroduced as an example for reference in measurements of motivation. If there was an agreement on its use among observers (Ethologists), which in reality is not the case, then empirical data may be collected, and the averages and spreads of parameter estimations will define a reference for an animal species. Later, observers with their own data will calibrate with the reference model, so that new observers will have calculated values of accuracy and precision for their data.

Research limitations/implications
Unlike hard science whose scales of measurement are practically unambiguous, measuring the purpose of behaviour of an animal has inherent ambiguity according to the reintroduced model. The ambiguity cannot be resolved from instantaneous readings. The necessary existence of ambiguity renders the criticism of hard science invalid, that of expecting to measure motivation with a static scale as if it were temperature.

Practical implications
Human observers can be treated as measuring devices of motivation from observing behaviour. Each observer can have characteristic accuracy/precision, or validity/reliability, calculated from empirical data.

Social implications
This is an inductive, rather than deductive, study of individual animal behaviour; the author believes it is extensible to individual human behaviour and personality studies. However, group behaviour studies are beyond its scope.

Originality/value
The author believes that the suggestion of ambiguity of scales of animal motivation is original, and the suggested link between SOC/RC and a mainstream hard science is new.
Paving the way for the circular economy and more sustainable supply chains
Authors: Cardoso de Oliveira Mara Cristina , Machado Marcio Cardoso , Chiappetta Jabbour Charbel Jose , Lopes de Sousa Jabbour Ana Beatriz 
Published: April 2019

PurposeCircular economy is an emerging concept which requires insights from a variety of disciplines, especially from sustainable operations management. Therefore, the purpose of this paper is to verify how formal and informal instruments of governance influence the induction of green practices in a green network located in Brazil, with implications for the circular economy.Design/methodology/approachBased on a review of the supply chain (SC), green supply chain management, and governance literature, proposals are made regarding the influence of governance instruments in inducing green practices. To investigate these propositions, a qualitative research was conducted using a single exemplary case study of a cosmetics supply network.FindingsThe authors present original research findings which have both expected and unexpected implications for the circular economy, due to the fact that the data analysis showed that the formal (contracts and environmental norms) and informal (trust and cooperation) instruments of governance positively influence the induction of green practices within the supply network.Originality/valueThis study contributes to supply network and governance theory by providing insights for better understanding of how governance instruments can induce green practices in a supply network, and it provides practical implications for SC managers, by showing the importance of considering different governance instruments. Implications for the circular economy are made.
Effects of food additives on gut microbiota: friend or foe?
Authors: Inan-Eroglu Elif , Ayaz Aylin 
Published: April 2019

PurposeRecent evidence suggests that especially processed foods may lead to undesirable metabolic effects in gut microbiota. The emulsifiers and artificial sweeteners that are added to processed foods may play a role in the progression of the diseases through the modulation of microbiota in mice. In this context, the purpose of this paper is to evaluate the effects of emulsifiers and artificial sweeteners.Design/methodology/approachThis paper presents a narrative review of the effects of emulsifiers and artificial sweeteners which are mainly in consumed in the Western diet, to the gut microbiota by mainly focusing on the experimental studies.FindingsAlthoughin vivostudies and animal model studies showed various adverse effects of sweeteners and emulsifiers to microbiota, studies should be conducted in humans to investigate the effects of these food additives to human microbiota by making dietary interventions in the context of ethical rules.Originality/valueIn future, studies will allow us to draw more definitive conclusion whether human population consuming sweeteners and emulsifiers are at risk.