bluuebunny/crossref_metadata_2025_split huggingface dataset:
When you go low, I go high: Negative coordination of physiological synchrony among parents and children
Authors: Creavy Kristine L. , Gatzke‐Kopp Lisa M. , Zhang Xutong , Fishbein Diana , Kiser Laurel J. 
Published: August 2019

AbstractThe parent–child relationship is an important context for children's emotional development. The current study assessed 43 parent–child dyads while watching a positive film clip and a negative film clip, in order to examine whether partner's physiological reactivity demonstrated dyadic synchrony, and whether this was associated with children's prosocial empathy. Respiratory sinus arrhythmia was measured in both caretakers and children (ages 9–14) and a continuous time series was estimated in order to maximize temporal resolution of physiological dynamics. Dyads varied in both the magnitude and direction of synchrony, with correlation coefficients ranging from moderately negative; r = −.44 (increases in one partner correspond to decreases in the other), to moderately positive; r = .55 (increases in one partner correspond to increases in the other). For both conditions, negative synchrony was correlated with higher child‐reported empathy. For the negative mood condition, this effect was moderated by caretaker's emotional acceptance such that positive synchrony was associated with lower levels of empathy among dyads in which caretaker emotional acceptance was lower. These results suggest that positive physiological synchrony during a negative mood condition may not be adaptive for children when their caretaker lacks the skills to appropriately socialize their child's emotional development.
Liraglutide combined with human umbilical cord mesenchymal stem cell transplantation inhibits beta‐cell apoptosis via mediating the ASK1/JNK/BAX pathway in rats with type 2 diabetes
Authors: Wang Wei , Wu Rong Dan , Chen Pin , Xu Xiang Jin , Shi Xiao Zhi , Huang Li Hong , Shao Zhu Lin , Guo Wen 
Published: August 2019

AbstractObjectiveAccumulating evidence suggests an association between beta‐cell apoptosis and the ASK1/JNK/BAX pathway. The aim of this study was to investigate the effects of a combined therapy of liraglutide and human umbilical cord mesenchymal stem cells (hUC‐MSCs) on the glucose metabolism and islet beta‐cell apoptosis, and further explore its relationship to the ASK1/JNK/BAX pathway.MethodType 2 diabetes mellitus (T2DM) rat model was induced by a high‐sugar and high‐fat diet and intraperitoneal injection of low‐dose streptozotocin (STZ) (30 mg/kg). Three days after STZ injection, diabetic rats were randomly treated with subcutaneous injection of liraglutide (200 μg/kg/12 h) for 8 weeks and or hUC‐MSCs (1 × 106/rat) at the first and fifth weeks. Diabetes‐related physical and biochemical parameters, pancreatic histopathological changes, immunohistochemical staining, quantitative real‐time polymerase chain reaction, and western blot were used to measure the expression of apoptosis signal‐regulating kinase 1 (ASK1), Jun N‐terminal kinase (JNK), Bcl‐2 associated X protein (BAX), and B‐cell lymphoma‐2 (Bcl‐2).ResultsEight weeks after liraglutide or human umbilical cord mesenchymal stem cell administration, FPG, HbA1c, glucagon, body weight, and pancreatic ASK1, JNK, and BAX mRNA and proteins were significantly decreased, and the levels of serum C‐p, INS and GLP‐1, ratio of insulin positive area, and Bcl‐2 expression were significantly increased in three treatment groups compared with T2DM group (P<.05).ConclusionLiraglutide combined with hUC‐MSCs improve glucose metabolism and inhibit islet beta‐cell apoptosis in a ASK1/JNK/BAX pathway‐dependent manner.
Electrochemical Detection of Guanine‐methylation Using Glassy Carbon Electrode
Authors: Mendes Carlos H. S. , Montenegro João G. S. , Queiroz Nathalia L. , Moreira Thayane C. S. , Nascimento Valberes B. , Oliveira Severino Carlos B. 
Published: August 2019

AbstractThis work presents a study of the electrochemical oxidation of 7‐methylguanine (7‐mGua) in aqueous solution at glassy carbon electrode by cyclic voltammetry, differential pulse voltammetry, square wave voltammetry and electrochemical impedance spectrometry. The anodic behaviour of 7‐mGua was compared with the electro‐oxidation of guanine and 7‐methylguanosine. The results demonstrated that the methyl and ribose groups are not electroactive but strongly influence the oxidation mechanism of these species. The oxidation of 7‐mGua occurred in a single pH‐dependent step, with the withdrawal of two electrons and two protons of C8, to form 8‐oxo‐7‐methylguanine, while the electro‐oxidation of 7‐methylguanosine also occurred in a single pH‐dependent step, however, with the withdrawal of one electron and one proton of C8 to form a hydroxylated product, since its oxidation to 8‐oxo‐7‐methylguanosine is hindered by the presence of the pendant groups. In addition, the oxidation of 7‐mGua was investigated in the presence of DNA and DNA‐bases, leading to the conclusion that the formation of 7‐mGua, from an interaction of DNA with an alkylating agent, would cause an increase on the deoxyguanosine peak current of the DNA‐biosensor, with no interference of any free DNA bases, which demonstrated that DNA‐electrochemical biosensors find application on detecting DNA methylation, opening a new avenue for applications of DNA biosensors.
Electrochemical Determination and in silico Studies of Fludarabine on NH2 Functionalized Multiwalled Carbon Nanotube Modified Glassy Carbon Electrode
Authors: Dogan‐Topal Burcu , Bakirhan Nurgul K. , Tok Tugba Taskin , Ozkan Sibel A. 
Published: August 2019

AbstractA sensitive voltammetric technique has been developed for the determination of Fludarabine using amine‐functionalized multi walled carbon nanotubes modified glassy carbon electrode (NH2‐MWCNTs/GCE). Molecular dynamics simulations, an in silico technique, were employed to examine the properties including chemical differences of Fludarabine‐ functionalized MWCNT complexes. The redox behavior of Fludarabine was examined by cyclic, differential pulse and square wave voltammetry in a wide pH range. Cyclic voltammetric investigations emphasized that Fludarabine is irreversibly oxidized at the NH2‐MWCNTs/GCE. The electrochemical behavior of Fludarabine was also studied by cyclic voltammetry to evaluate both the kinetic (ks and Ea) and thermodynamic (ΔH, ΔG and ΔS) parameters on NH2‐MWCNTs/GCE at several temperatures. The mixed diffusion‐adsorption controlled electrochemical oxidation of Fludarabine revealed by studies at different scan rates. The experimental parameters, such as pulse amplitude, frequency, deposition potential optimized for square‐wave voltammetry. Under optimum conditions in phosphate buffer (pH 2.0), a linear calibration curve was obtained in the range of 2×10−7 M–4×10−6 M solution using adsorptive stripping square wave voltammetry. The limit of detection and limit of quantification were calculated 2.9×10−8 M and 9.68×10−8 M, respectively. The developed method was applied to the simple and rapid determination of Fludarabine from pharmaceutical formulations.
Hedging performance of multiscale hedge ratios
Authors: Sultan Jahangir , Alexandridis Antonios K. , Hasan Mohammad , Guo Xuxi 
Published: August 2019

AbstractIn this study, the wavelet multiscale model is applied to selected assets to hedge time‐dependent exposure of an agent with a preference for a certain hedging horizon. Based on the in‐sample and out‐of‐sample portfolio variances, the wavelet‐based generalized autoregressive conditional heteroskedasticity (GARCH) model produces the lowest variances. From a utility standpoint, wavelet networks combined with GARCH have the highest utility. Finally, the wavelet‐GARCH model has the lowest minimum capital risk requirements. Overall, the wavelet GARCH and wavelet networks offer improvements over traditional hedging models.
Immune Exhaustion of T Cells in Alveolar Echinococcosis Patients and Its Reversal by Blocking Checkpoint Receptor TIGIT in a Murine Model
Authors: Zhang Chuanshan , Lin Renyong , Li Zhide , Yang Shuting , Bi Xiaojuan , Wang Hui , Aini Abudusalamu , Zhang Ning , Abulizi Abuduaini , Sun Cheng , Li Liang , Zhao Zhibin , Qin Rongde , Li Xiaohong , Li Liang , Aji Tuerganaili , Shao Yingmei , Vuitton Dominique A. , Tian Zhigang , Wen Hao 
Published: August 2019

Background and Aims
The cestode Echinococcus multilocularis infection, a serious health problem worldwide, causes alveolar echinococcosis (AE), a tumor‐like disease predominantly located in the liver and able to spread to any organs. Until now, there have been few studies that explore how T‐cell exhaustion contributes to the parasite’s escape from immune attack and how it might be reversed.

Approach and Results
In this study, we found that liver T‐cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine‐based inhibitory motif domain (TIGIT) expression was significantly enhanced and positively correlated with lesion activity in AE patients. High TIGIT expression in both liver‐infiltrating and blood T cells was associated with their functional exhaustion, and its ligand CD155 was highly expressed by hepatocytes surrounding the infiltrating lymphocytes. In co‐culture experiments using human blood T cells and hepatic cell line HL‐7702, CD155 induced functional impairment of TIGIT+ T cells, and in vitro blockade with TIGIT antibody restored the function of AE patients’ T cells. Similar TIGIT‐related functional exhaustion of hepatic T cells and an abundant CD155 expression on hepatocytes were observed in E. multilocularis–infected mice. Importantly, in vivo blocking TIGIT prevented T‐cell exhaustion and inhibited disease progression in E. multilocularis–infected mice. Mechanistically, CD4+ T cells were totally and CD8+ T cells partially required for anti‐TIGIT–induced regression of parasite growth in mice.

Conclusions
This study demonstrates that E. multilocularis can induce T‐cell exhaustion through inhibitory receptor TIGIT, and that blocking this checkpoint may reverse the functional impairment of T cells and represent a possible approach to immunotherapy against AE.
Novel Lipid Long Intervening Noncoding RNA, Oligodendrocyte Maturation‐Associated Long Intergenic Noncoding RNA, Regulates the Liver Steatosis Gene Stearoyl‐Coenzyme A Desaturase As an Enhancer RNA
Authors: Benhammou Jihane N. , Ko Arthur , Alvarez Marcus , Kaikkonen Minna U. , Rankin Carl , Garske Kristina M. , Padua David , Bhagat Yash , Kaminska Dorota , Kärjä Vesa , Pihlajamäki Jussi , Pisegna Joseph R. , Pajukanta Päivi 
Published: August 2019

The global obesity epidemic is driving the concomitant rise in nonalcoholic fatty liver disease (NAFLD). To identify new genes involved in central liver functions, we examined liver RNA‐sequence data from 259 patients who underwent morbidly obese bariatric surgery. Of these patients, 84 had normal liver histology, 40 simple steatosis, 43 nonalcoholic steatohepatitis, and the remaining 92 patients had varying degrees of NAFLD based on liver histology. We discovered oligodendrocyte maturation‐associated long intergenic noncoding RNA (OLMALINC), a long intervening noncoding RNA (lincRNA) in a human liver co‐expression network (n = 75 genes) that was strongly associated with statin use and serum triglycerides (TGs). OLMALINC liver expression was highly correlated with the expression of known cholesterol biosynthesis genes and stearoyl‐coenzyme A desaturase (SCD). SCD is the rate‐limiting enzyme in monounsaturated fatty acids and a key TG gene that is known to be up‐regulated in liver steatosis and NAFLD and resides adjacent to OLMALINC on the human chromosome 10q24.31. Next, we functionally demonstrated that OLMALINC regulates SCD as an enhancer‐RNA (eRNA), thus describing the first lincRNA that functions as an eRNA to regulate lipid metabolism. Specifically, we show that OLMALINC promotes liver expression of SCD in cis through regional chromosomal DNA–DNA looping interactions. Conclusion: The primate‐specific lincRNA OLMALINC is a novel epigenetic regulator of the key TG and NAFLD gene SCD.
Unconditional nonlinear stability for double‐diffusive convection in a porous medium with temperature‐dependent viscosity and density
Authors: Hameed Ayat A. , Harfash Akil J. 
Published: August 2019

AbstractIn this study, fluid flow in a porous medium is analyzed using a Forchheimer model. The problem of double‐diffusive convection is addressed in such a porous medium. We utilize a higher‐order approximation for viscosity‐temperature and density‐temperature, such that the perturbation equations contain more nonlinear terms. For unconditional stability, nonlinear stability has been achieved for all initial data by utilizing the or norms. It also shows that the theory of is not sufficient for such unconditional stability. Both linear instability and nonlinear energy stability thresholds are tested using three‐dimensional (3D) simlations. If the layer is salted above and salted below then stationary convection is dominant. Thus the critical value of the linear instability thresholds occurs at a real eigenvalue , and our results show that the linear theory produces the actual threshold. Moreover, it is known that with the increase of the salt Rayleigh number, Rc, the onset of convection is more likely to be via oscillatory convection as opposed to steady convection. The 3D simulation results show that as the value of Rc increases, the actual threshold moves towards the nonlinear stability threshold, and the behavior of the perturbation of the solutions becomes more oscillatory.
Effects of double stratification on MHD chemically reacting second‐grade fluid through porous medium between two parallel plates
Authors: Kumar N. Naresh , Ojjela Odelu , Sastry D. R. V. S. R. K. 
Published: August 2019

AbstractThe present study focuses on the study of heat transfer in a magnetohydrodynamic double stratification fluid flow through porous channel. In getting the solution of Navier‐Stokes equations, we have adapted the similarity transformation technique to convert the partial differential equations into a coupled system of nonlinear ordinary differential equations. We further applied the fourth‐order Runge‐Kutta method with shooting technique to solve the problem numerically. The results are exhibited via graphs, and the analysis has been addressed for various flow characteristics with respect to the parameters concerned. It is found that thermal stratification enhances both temperature and heat transfer rates, and solutal stratification enhances concentration and mass transfer rates. Concentration decreases with increasing chemical reaction and it increases with stratification. A significant variation is observed between concentration profiles in the presence of stratification.
Developing Disciplinary Literacy in Mathematics: Learning From Professionals Who Use Mathematics in Their Jobs
Authors: Croce Keri‐Anne , K. McCormick Montana 
Published: August 2019

AbstractPedagogy within the field of disciplinary literacy can be shaped by those who use mathematics in their jobs. The authors draw on the results of a study that investigated language used by professionals who use mathematics in their jobs. The findings of the study suggest that professionals use language to determine the change requested by a client, create a solution, and present the solution within a narrative crafted for a specific audience. In addition, professionals are members of different language communities. The construct of a language community involves defining how roles and power structures specifically shape audiences for disciplinary language. The authors offer recommendations as to how student apprentices may examine language as they enter into language communities with professionals who become specialists/mentors. Students can also practice using language for specific purposes through a process called linguistic inquiry and discourse analysis.
In vivo hydroxyapatite scaffold performance in infected bone defects
Authors: Pearson Joseph J. , Gerken Nicholas , Bae Chunsik , Lee Kyu‐Bok , Satsangi Arpan , McBride Sean , Appleford Mark R. , Dean David D. , Hollinger Jeffrey O. , Ong Joo L. , Guda Teja 
Published: August 2019

AbstractCritically sized bone defects are often compounded by infectious complications. The standard of care consists of bone autografts with systemic antibiotics. These injuries and treatments lead to donor site morbidity, antibiotic resistant strains of bacteria, and often end stage amputation. This study proposes an alternative to the autograft using a porous, hydroxyapatite (HA) scaffold evaluated with and without infection and antibiotics. Twenty‐four New Zealand white rabbits received either our HA scaffold or a pulverized autograft (PBA) within a surgically created critical‐sized defect in the femur. The two grafts were evaluated in either septic or aseptic defects and with or without antibiotic treatment. The HA scaffolds were characterized with micro computed tomography. Post‐euthanasia, micro computed tomography, histology, and white blood cells component analysis were completed. The HA had significantly greater (p < .001) mineralization to total volume than the PBA groups with 27.56% and 14.88%, respectively, and the septic HA groups were significantly greater than the aseptic groups both with and without antibiotics (p = .016). The bone quality denoted by bone mineral density was also significantly greater (p < .001) in the HA groups (67.01 ± 0.38 mgHA/cm3) than the PBA groups (64.66 ± 0.85 mgHA/cm3). The HA scaffold is a viable alternative to the bone autograft in defects with and without infection as shown by the quality and quantity of bone.
Fracture and Bone Mineral Density Response by Baseline Risk in Patients Treated With Abaloparatide Followed by Alendronate: Results From the Phase 3 ACTIVExtend Trial
Authors: Leder Benjamin Z , Zapalowski Carol , Hu Ming-Yi , Hattersley Gary , Lane Nancy E , Singer Andrea J , Dore Robin K 
Published: August 2019

ABSTRACT
In the randomized, placebo-controlled, double-blind phase 3 ACTIVE study (NCT01343004), 18 months of abaloparatide 80 μg daily (subcutaneous injection) in postmenopausal women at risk of osteoporotic fracture significantly reduced the risk of vertebral, nonvertebral, clinical, and major osteoporotic fractures and significantly increased bone mineral density (BMD) versus placebo regardless of baseline risk factors. Women from the abaloparatide and placebo groups who completed ACTIVE were eligible for ACTIVExtend (NCT01657162), in which all enrollees received sequential, open-label monotherapy with alendronate 70 mg once weekly for up to 24 months. This prespecified analysis evaluated whether fracture risk reductions and bone mineral density (BMD) gains associated with abaloparatide during ACTIVE persisted through the full 43-month ACTIVE–ACTIVExtend study period in nine prespecified baseline risk subgroups. Baseline risk subgroups included BMD T-score at the lumbar spine, total hip, and femoral neck (≤ − 2.5 versus &gt; − 2.5 and ≤ −3.0 versus &gt; − 3.0), history of nonvertebral fracture (yes/no), prevalent vertebral fracture (yes/no), and age (&lt;65 versus 65 to &lt;75 versus ≥75 years). Forest plots display treatment effect. Treatment-by-subgroup interactions were tested using the Breslow-Day test, Cox proportional hazards model, and ANCOVA model. After the combined ACTIVE–ACTIVExtend study period, reductions in relative risk for new vertebral, nonvertebral, clinical, and major osteoporotic fractures were greater among patients in the abaloparatide/alendronate group than among those in the placebo/alendronate group across all nine baseline risk subgroups. BMD gains at the lumbar spine, total hip, and femoral neck were greater in the abaloparatide/alendronate group versus the placebo/alendronate group. No clinically meaningful interaction between treatment assignment and any baseline risk variable was observed. The sequence of abaloparatide for 18 months followed by alendronate for up to 24 months appears to be an effective treatment option for a wide range of postmenopausal women at risk for osteoporotic fractures. © 2019 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals, Inc.
Preliminary study: Descriptive analysis of longitudinal data on the international session of the Japan Primary Care Association annual conference, 2012–2018
Authors: Hirooka Nobutaka , Ban Nobutaro ,   the Japan Primary Care Association International Affairs Committee
Published: August 2019

AbstractIntroductionJapan Primary Care Association has launched an international session at annual conference since 2012. Longitudinal characteristics of the presentations were investigated.MethodsAbstracts of the session were analyzed regarding type of presentation (oral/poster), category (research/clinical case/activity/review), and presenters' geographical location.ResultsThe numbers of oral and poster presentations significantly increased. The most frequent presentation was research with cross‐sectional survey. Majority of presentations were clinical and less educational research, most of which were by Japanese.ConclusionThe numbers of presentations in the international session increased, but the session should be used more rigorously to enhance primary care.
External validation and update of a prognostic model to predict mortality in hospitalized adults with RSV: A retrospective Dutch cohort study
Authors: Vos Laura M. , Oosterheert Jan Jelrik , Hoepelman Andy I. M. , Bont Louis J. , Coenjaerts Frank E. J. , Naaktgeboren Christiana A. 
Published: August 2019

AbstractRespiratory syncytial virus (RSV) causes significant mortality in hospitalized adults. Prediction of poor outcomes improves targeted management and clinical outcomes. We externally validated and updated existing models to predict poor outcome in hospitalized RSV‐infected adults. In this single center, retrospective, observational cohort study, we included hospitalized adults with respiratory tract infections (RTIs) and a positive polymerase chain reaction for RSV (A/B) on respiratory tract samples (2005‐2018). We validated existing prediction models and updated the best discriminating model by revision, recalibration, and incremental value testing. We included 192 RSV‐infected patients (median age 60.7 years, 57% male, 65% immunocompromised, and 43% with lower RTI). Sixteen patients (8%) died within 30 days. During hospitalization, 16 (8%) died, 30 (16%) were admitted to intensive care unit, 21 (11%) needed invasive mechanical ventilation, and 5 (3%) noninvasive positive pressure ventilation. Existing models performed moderately at external validation, with C‐statistics 0.6 to 0.7 and moderate calibration. Updating to a model including lower RTI, chronic pulmonary disease, temperature, confusion and urea, increased the C‐statistic to 0.76 (95% confidence interval, 0.61‐0.91) to predict in‐hospital mortality. In conclusion, existing models to predict poor prognosis among hospitalized RSV‐infected adults perform moderately at external validation. A prognostic model may help to identify and treat RSV‐infected adults at high‐risk of death.