bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mixed-lineage leukemia protein 2 suppresses ciliary assembly by the modulation of actin dynamics and vesicle transport
Authors: Yang Yang , Hao Huijie , Wu Xiaofan , Guo Song , Liu Yang , Ran Jie , Li Te , Li Dengwen , Liu Min , Zhou Jun 
Published: June 2019

AbstractPrimary cilia are critically involved in the coordination of diverse signaling pathways and ciliary defects are associated with a variety of human diseases. The past decades have witnessed great progress in the core machinery orchestrating ciliary assembly. However, the upstream epigenetic cues that direct ciliogenesis remain elusive. Herein, we demonstrate that mixed-lineage leukemia protein 2 (MLL2), a histone methyltransferase, plays a negative role in ciliogenesis. RNA-sequencing analysis reveals that the expression of five actin-associated proteins is significantly downregulated in MLL2-depleted cells. Overexpression of these proteins partially rescues ciliary abnormality elicited by MLL2 depletion. Our data also show that actin dynamics is remarkably changed in MLL2-depleted cells, resulting in the impairment of cell adhesion, spreading, and motility. In addition, MLL2 depletion promotes ciliary vesicle trafficking to the basal body in an actin-related manner. Together, these results reveal that MLL2 inhibits ciliogenesis by modulating actin dynamics and vesicle transport, and suggest that alteration of MLL2 may contribute to the pathogenesis of cilium-associated diseases.
Circuit asymmetries underlie functional lateralization in the mouse auditory cortex
Authors: Levy Robert B. , Marquarding Tiemo , Reid Ashlan P. , Pun Christopher M. , Renier Nicolas , Oviedo Hysell V. 
Published: June 2019

AbstractThe left hemisphere’s dominance in processing social communication has been known for over a century, but the mechanisms underlying this lateralized cortical function are poorly understood. Here, we compare the structure, function, and development of each auditory cortex (ACx) in the mouse to look for specializations that may underlie lateralization. Using Fos brain volume imaging, we found greater activation in the left ACx in response to vocalizations, while the right ACx responded more to frequency sweeps. In vivo recordings identified hemispheric differences in spectrotemporal selectivity, reinforcing their functional differences. We then compared the synaptic connectivity within each hemisphere and discovered lateralized circuit-motifs that are hearing experience-dependent. Our results suggest a specialist role for the left ACx, focused on facilitating the detection of specific vocalization features, while the right ACx is a generalist with the ability to integrate spectrotemporal features more broadly.
Organometallic compounds as carriers of extraterrestrial cyanide in primitive meteorites
Authors: Smith Karen E. , House Christopher H. , Arevalo Ricardo D. , Dworkin Jason P. , Callahan Michael P. 
Published: June 2019

AbstractExtraterrestrial delivery of cyanide may have been crucial for the origin of life on Earth since cyanide is involved in the abiotic synthesis of numerous organic compounds found in extant life; however, little is known about the abundance and species of cyanide present in meteorites. Here, we report cyanide abundance in a set of CM chondrites ranging from 50 ± 1 to 2472 ± 38 nmol·g−1, which relates to the degree of aqueous alteration of the meteorite and indicates that parent body processing influenced cyanide abundance. Analysis of the Lewis Cliff 85311 meteorite shows that its releasable cyanide is primarily in the form of [FeII(CN)5(CO)]3− and [FeII(CN)4(CO)2]2−. Meteoritic delivery of iron cyanocarbonyl complexes to early Earth likely provided an important point source of free cyanide. Iron cyanocarbonyl complexes may have served as precursors to the unusual FeII(CN)(CO) moieties that form the catalytic centers of hydrogenases, which are thought to be among the earliest enzymes.
Disentangling the relative impacts of climate change and human activities on fluvial sediment supply to the coast by the world’s large rivers: Pearl River Basin, China
Authors: Ranasinghe Roshanka , Wu Chuang Shou , Conallin John , Duong Trang Minh , Anthony Edward Jamal 
Published: June 2019

AbstractThe world’s large rivers are under stress and experiencing unprecedented changes in hydrology, ecosystems, and fluvial sediment loads. Many of these rivers terminate at the great deltas of the world (home to 500 million people), which depend on fluvial sediments for their very existence. While fluvial sediment loads of large rivers have already been greatly modified by human activities, climate change is expected to further exacerbate the situation. But how does the effect of climate change on fluvial sediment loads compare with that of human impacts? Here, we address this question by combining historical observations and 21st century projections for one of the world’s largest 25 rivers containing two mega dams; Pearl River, China. Our analysis shows that variations in fluvial sediment supply to the coast from the Pearl river over a ~150 year study period are dominated by human activities. Projected climate change driven 21st century increases in riverflow will only compensate for about 1% of the human induced deficit in sediment load, leading to the coastal zone being starved of about 6000 Mt of sediment over the remainder of this century. A similar dominance of human impacts on fluvial sediment supply is likely at other heavily engineered rivers.
Purification and characterization of a novel, highly potent fibrinolytic enzyme from Bacillus subtilis DC27 screened from Douchi, a traditional Chinese fermented soybean food
Authors: Hu Yuanliang , Yu Dan , Wang Zhaoting , Hou Jianjun , Tyagi Rohit , Liang Yunxiang , Hu Yongmei 
Published: June 2019

AbstractThe highly fibrinolytic enzyme-producing bacterium was identified as Bacillus subtilis DC27 and isolated from Douchi, a traditional fermented soybean food. The DFE27 enzyme was purified from the fermentation broth of B. subtilis DC27 by using UNOsphere Q column chromatography, Sephadex G-75 gel filtration, and high-performance liquid chromatography. It was 29 kDa in molecular mass and showed the optimal reaction temperature and pH value of 45 °C and 7.0, respectively, with a stable fibrinolytic activity below 50 °C and within the pH range of 6.0 to 10.0. DFE27 was identified as a serine protease due to its complete inhibition by phenylmethysulfony fluoride. The first 24 amino acid residues of the N-terminal sequence of the enzyme were AQSVPYGVSQIKAPALHSQGFTGS. The enzyme displayed the highest specificity toward the substrate D-Val-Leu-Lys-pNA for plasmin and it could not only directly degrade but also hydrolyze fibrin by activating plasminogen into plasmin. Overall, the DFE27 enzyme was obviously different from other known fibrinolytic enzymes in the optimum substrate specificity or fibrinolytic action mode, suggesting that it is a novel fibrinolytic enzyme and may have potential applications in the treatment and prevention of thrombosis.
Local accumulation of diacylglycerol alters membrane properties nonlinearly due to its transbilayer activity
Authors: Campomanes Pablo , Zoni Valeria , Vanni Stefano 
Published: June 2019

AbstractDiacylglycerols (DAGs) are bioactive lipids that are ubiquitously present at low concentrations in cellular membranes. Upon the activation of lipid remodeling enzymes such as phospholipase C and phosphatidic acid phosphatase, DAG concentration increases, leading to a disruption of the lamellar phase of lipid membranes. To investigate the structural origin of these phenomena, here we develop a coarse-grained model for DAGs that is able to correctly reproduce its physicochemical properties, including interfacial tension and flip-flop rate. We find that even at low concentrations a nonnegligible percentage of DAG molecules occupies the interleaflet space. At high concentrations, DAG molecules undergo a phase-separation process from lamellar lipids, segregating in DAG-only blisters and effectively reducing the DAG surface pool available to peripheral enzymes. Our results allow for a better understanding of the role of DAGs in cellular membranes and provide a new tool for the quantitative estimation of low-abundance lipids on membrane properties.
Compound phenotype of osteogenesis imperfecta and Ehlers–Danlos syndrome caused by combined mutations inCOL1A1andCOL5A1
Authors: Lin Zejia , Zeng Jican , Wang Xinjia 
Published: June 2019

AbstractOsteogenesis imperfecta (OI) is an inherited connective tissue disorder with a broad clinical spectrum that can overlap with Ehlers–Danlos syndrome (EDS). To date, patients with both OI and EDS have rarely been reported. In the present study, we investigated a family with four members, one healthy individual, one displaying OI only, and two displaying the compound phenotype of OI and EDS, and identified the pathogenic mutations. Whole exome sequencing was applied to the proband and her brother. To verify that the mutations were responsible for the pathogenesis, conventional Sanger sequencing was performed for all members of the family. We identified a known COL1A1 (encoding collagen type I α 1 chain) mutation (c.2010delT, p.Gly671Alafs*95) in all three patients (the proband, her brother, and her mother) in this family, but also a novel heterozygous COL5A1 (encoding collagen type V α 1 chain) mutation (c.5335A&gt;G, p.N1779D) in the region encoding the C-terminal propeptide domain in the proband and her mother, who both had the compound phenotype of OI and EDS. The results of the present study suggested that the proband and her mother presented with the compound OI–EDS phenotype caused by pathogenic mutations in COL5A1 and COL1A1.
Magnetic probe for improvement of near‐field resolution in radiated susceptibility mapping
Authors: Merfeldas A. , Kuzas P. , Gailius D. , Nakutis Z. 
Published: June 2019

In this Letter, a magnetic near‐field probe aimed for improvement of the spatial resolution of radiated susceptibility mapping over the 80–3000 MHz frequency range is presented. The magnetic field simulation of the standard and the improved probe was performed and the measurement results for both probes are presented. The spatial resolution improvement of up to 47% was shown by modelling. The measured resolution improvement of 38.5% confirmed the enhanced localisation of susceptible RF PCB areas using the proposed probe.
The EFSUMB Guidelines and Recommendations for the Clinical Practice of Elastography in Non-Hepatic Applications: Update 2018
Authors: Săftoiu Adrian , Gilja Odd Helge , Sidhu Paul S. , Dietrich Christoph F. , Cantisani Vito , Amy Dominique , Bachmann-Nielsen Michael , Bob Flaviu , Bojunga Jörg , Brock Marko , Calliada Fabrizio , Clevert Dirk André , Correas Jean-Michel , D’Onofrio Mirko , Ewertsen Caroline , Farrokh André , Fodor Daniela , Fusaroli Pietro , Havre Roald Flesland , Hocke Michael , Ignee André , Jenssen Christian , Klauser Andrea Sabine , Kollmann Christian , Radzina Maija , Ramnarine Kumar V. , Sconfienza Luca Maria , Solomon Carolina , Sporea Ioan , Ștefănescu Horia , Tanter Mickael , Vilmann Peter 
Published: June 2019

AbstractThis manuscript describes the use of ultrasound elastography, with the exception of liver applications, and represents an update of the 2013 EFSUMB (European Federation of Societies for Ultrasound in Medicine and Biology) Guidelines and Recommendations on the clinical use of elastography.
Perioperative Complications after Kasai Hepatoportoenterostomy: Data from the Swiss National Biliary Atresia Registry
Authors: Calinescu Ana M. , Wilde Jim C. H. , Korff Simona , McLin Valérie A. , Wildhaber Barbara E. 
Published: June 2019

Introduction Hepatoportoenterostomy (HPE) is the first-line treatment for biliary atresia (BA) patients. This study aims to describe perioperative complications after HPE and to analyze their impact on outcome.
Materials and Methods Patients with HPE (Swiss National Biliary Atresia Registry, 1994–2017) were retrospectively analyzed. Perioperative complications were defined as complications occurring up to 30 days after surgery. Surgical complications were defined as directly related to the surgical act; medical complications were defined as any other deviation from the uneventful postoperative course.
Results Sixty-two patients were included. Median age at HPE was 63 days (18–126). Twenty six patients out of 62 (42%) had ≥ 1 complications: 6/62 (10%) surgical, 24/62 (39%) medical, that is, we observed 7 surgical and 28 medical complications. As for medical complications, cholangitis was the most frequent: 19/28 (68%). Lower gestational age at birth correlated with more overall complications (p = 0.02). Age, weight at HPE, syndromic BA, and postoperative steroid administration were not significantly correlated. There were no perioperative deaths. Perioperative complications did not correlate with overall survival (p = 0.14) and survival with native liver (p = 0.55).
Conclusion HPE is often associated with perioperative medical complications. Lower gestational age at birth was significantly associated with more complications. Perioperative complications had no impact on overall outcome.
Classification of degenerations and Picard lattices of Kählerian K3 surfaces with symplectic automorphism group
Authors: Nikulin V. V. 
Published: June 2019

Abstract
In [1]–[6] we classified the degenerations and Picard lattices of Kählerian K3 surfaces with finite symplectic automorphism groups of high order. This classification was not considered for the remaining groups of small order (

,

,

,

,

and

) because each of these cases requires very long and difficult considerations and calculations.
Here we consider this classification for the dihedral group

of order

.
Strigolactone promotes cytokinin degradation through transcriptional activation ofCYTOKININ OXIDASE/DEHYDROGENASE 9in rice
Authors: Duan Jingbo , Yu Hong , Yuan Kun , Liao Zhigang , Meng Xiangbing , Jing Yanhui , Liu Guifu , Chu Jinfang , Li Jiayang 
Published: June 2019

SignificanceStrigolactone plays a vital role in plant growth and development, but its response genes remain to be identified. In this study, we found that cytokinin content is markedly increased in the strigolactone signaling mutantd53, and thatOsCKX9, which encodes a cytokinin oxidase to catalyze the degradation of cytokinin, functions as a primary strigolactone-responsive gene to regulate rice tillering, plant height, and panicle size, likely via a secondary response gene,OsRR5, which encodes a cytokinin-inducible rice type-A response regulator, demonstrating that strigolactone regulates rice shoot architecture through enhanced cytokinin catabolism by modulatingOsCKX9expression.
Americans overestimate the intergenerational persistence in income ranks
Authors: Cheng Siwei , Wen Fangqi 
Published: June 2019

SignificanceIntergenerational mobility indicates the openness within a society. The question of how Americans think about socioeconomic mobility prospects is drawing growing attention from scholars and policy makers. Our study proposes a survey instrument that connects the empirical literature on patterns of mobility with the literature on the public perceptions of mobility. With large-scale, population-representative data, we show that Americans overestimate the intergenerational persistence in income ranks. That is, they tend to see greater inequality of economic prospects between children from rich and poor families. These results highlight the need for policy and political solutions that seriously engage with Americans’ concerns about the equality of opportunity in the society.
Multimodal cue integration in the dung beetle compass
Authors: Dacke Marie , Bell Adrian T. A. , Foster James J. , Baird Emily J. , Strube-Bloss Martin F. , Byrne Marcus J. , el Jundi Basil 
Published: June 2019

Significance
We show that South African dung beetles use a multimodal orientation compass based on celestial cues and wind cues to steer across the savanna. The cue preference between these 2 modalities is set in a flexible manner: at low sun elevations, the beetles use a celestially driven compass, but switch to a wind-dominated compass at high sun elevations. This switch allows the beetles to orient with high precision even when the midday sky no longer provides reliable directional input. Moreover, our data suggest that the wind and celestial cues converge in the same memory network in the brain. This spatial memory based on both modalities allows the animal to rely on the most reliable cue at any moment in time.
Integration of renewable deep eutectic solvents with engineered biomass to achieve a closed-loop biorefinery
Authors: Kim Kwang Ho , Eudes Aymerick , Jeong Keunhong , Yoo Chang Geun , Kim Chang Soo , Ragauskas Arthur 
Published: June 2019

Significance
Deep eutectic solvents (DESs) have gained increasing attention due to their application-friendly properties, including universal solvating capabilities and wide tunability. Additionally, ease of synthesis and broad availability from inexpensive chemical components could render DESs more versatile solvents for biomass pretreatment, as compared with traditional ionic liquids. Because the long-term success of the biorefinery depends on the development of sustainable processes to convert lignocellulosics into biofuels, DESs derived from renewable sources such as lignin are highly desirable. We herein present our innovative process that integrates the use of low-recalcitrant engineered biomass with its pretreatment using lignin-derived DESs. The promising results described by near-theoretical sugar yield demonstrate the effectiveness of the integrated process, opening up opportunities toward a sustainable and circular bioeconomy.
Structure-guided examination of the mechanogating mechanism of PIEZO2
Authors: Taberner Francisco J. , Prato Vincenzo , Schaefer Irina , Schrenk-Siemens Katrin , Heppenstall Paul A. , Lechner Stefan G. 
Published: June 2019

Significance
Aristotle’s five senses—sight, smell, taste, hearing, and touch—are crucial for the perception of and interaction with the environment. While the molecular machineries that detect visual, olfactory, gustatory, and auditory stimuli are well characterized, comparatively little is known about how the sense of touch works at the molecular level. Here we identified and characterized intraprotein domain interfaces that govern the mechanosensitivity of the ion channel PIEZO2. Considering that PIEZO2 is an important detector of tactile and painful mechanical stimuli, selectively blocking these domain interactions in primary afferent neurons might provide an effective means to modulate pain sensitivity. Thus our work provides the basis for the development of additional types of analgesics that attack pain at its source.
Le discours préfaciel au XVIIIe siècle à travers le Dictionnaire de
l’Académie (1718) et le Dictionnaire de Trévoux (1721). Essai de
classification
Authors: Trujillo-González Verónica C. 
Published: June 2019

Abstract
The 18th century was a very productive period from a lexicographic point of view. In that century, the French
Academy published four new editions of their dictionary, being the second edition (1718) the one that included major revisions (1718). The Dictionnaire de
Trévoux (1721) is also considered to be one of the pillars of 18th century
lexicography in France, with eight published editions. The comparison of the prefaces of these two major pieces of French
lexicography, in spite of their different conceptions, will allow us to establish the big strategical lines that have marked
French Lexicography during the first part of the 18th century, as well as presenting how two of the most important dictionaries of
the French 18th century are organized.
Electron spectroscopy of various nanostructured carbon films
Authors: Nishchak O Yu , Savchenko N F , Streletsky O A , Zykova E Yu , Khvostov V V 
Published: June 2019

Abstract
X-ray photoelectron spectroscopy technique have been used to study carbon films obtained by means of ion-plasma deposition in the plasma of different chemical composition. The detailed analysis of electron spectroscopy data made it possible to determine the structure of the films and the influence of nitrogen and hydrogen admixture in plasma on the structural properties. Diamond-like films are obtained in pure argon plasma, graphite-like and carbon chain structures correspond to nitrogen and hydrogen addition respectively.
Formation and investigation of plasma nanostructured hafnia coatings
Authors: Savushkina S V , Polyansky M N , Vysotina E A , Ashmarin A A , Panasova G V , Agureev L E 
Published: June 2019

Abstract
Nanostructured coatings HfO2-9wt%Y2O3 were obtained by plasma spraying in vacuum using supersonic nozzle creating Prandtl-Mayer expansion fan. The coatings were investigated by scanning electron microscopy, X-ray diffraction analysis, and synchronous thermal analysis (differential scanning calorimetry, thermogravimetric analysis, and differential thermogravimetric analysis) in the temperature range of 40÷1600°C. The coatings are predominantly characterized by the nanostructured globular structure. Thermal analysis showed a peak corresponding to the exothermic reaction in the temperature range of 1400÷1600 °C, accompanied by a mass loss. c-HfO2 phase remained in the coating after thermal analysis with a decrease in the lattice parameter a from 5.18 Å to 5.13 Å.
Total reflectance of a random medium with the Reynolds-McCormick phase function at grazing incidence of light
Authors: Marinyuk V V , Sheberstov S V 
Published: June 2019

Abstract
We study the total reflectance of an absorbing, multiply scattering medium with large (as compared to the light wavelength) inhomogeneities at grazing incidence of light. To model highly forward scattering in the medium, we take advantage of the two-parameter Reynolds-McCormick scattering phase function. Using the scaling analysis for the small-angle radiative transfer equation, we derive simple analytic formulae for the dependence of the reflectance on the medium transport coefficients and the angle of incidence. The results obtained are verified by comparison with results of a direct numerical integration of the radiative transfer equation.
Structure and tribological behavior of electro-spark deposited TiC based coatings
Authors: Medts G De , Petrzhik M I , Kiryukhantsev-Korneev P V 
Published: June 2019

Abstract
This work gives an analysis of the coating obtained by electro-spark alloying using TiCNiCr electrode regarding their composition, structure, morphology, roughness, and mechanical properties. Tribological properties were studied by pin-on-disk test against different counterparts without lubricant. Soft materials like Cu, 100Cr6 steel are not damaging the coating and hard materials are either a good couple like with WC-Co, or characterized by a aggressive wear like with SiC, Si3N4, Al2O3. Detailed microscopic investigations of the tracks were done after tribo-tests.
Experimental study of environment ionization in the zone of a periodic discharge in a flow of liquid (PDFL)
Authors: Bogdanovich B Yu , Buyanov G O , Nesterovich A V 
Published: June 2019

Abstract
The study of the ionized environment in the area surrounding PDFL has showed the presence of visible plasmoids moving in the horizontal plane. The study of traces on the X-ray film made it possible to detect a significant number of traces of an identical shape and size that look like the “birds” which were found earlier in other facilities. A preliminary analysis has been carried out for their identification, which led to a conclusion about the nature of these formations, in particular, their identity with the Dirac monopole (or similar object) having a “tail” and “wings” formed by a flow of electrons from the surroundings.