bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scaffolding of NLRP3 on osmotically swollen vesicles but not intermediate filaments promotes inflammasome assembly
Authors: de Paz Linares Guillermo , Mossemann Jan , Sayed Blayne , Freeman Spencer A. 
Published: November 2024

AbstractInflammasomes are nucleated by receptors that become activated upon cellular stresses including ionic dyshomeostasis. Rather than forming in the cytosol, recent evidence suggests that inflammasomes are nucleated at specific sites in the cell including on cytoskeletal polymers and the membrane surfaces of organelles. The NLRP3 inflammasome, which is formed upon the loss of cytosolic K+, had been proposed to form on intermediate filaments as well as on vesicles derived from the trans-Golgi network and endosomes. To determine the necessary requirement of either mechanism, we used vimentin knockout macrophages which do not have intermediate filaments and compared the formation and function of NLRP3 inflammasomes. We report that vimentin was dispensable for the activation of caspase-1, IL-β cleavage and release, and the inflammatory responses in mice which are attributed to the inflammasome. Instead, NLRP3 was found on osmotically swollen PI4P-and LAMP1-positive compartments. These results provide clarification to the two models for NLRP3 inflammasome formation, demonstrating that intermediate filaments are not required, which contrasts with select previous literature. The results also suggest that NLRP3 may be activated by signals coincident with PI4P, including high membrane tension on vesicular compartments.
High-brightness megahertz-rate beam from a direct-current and superconducting radio-frequency combined photocathode gun
Authors: Jia H. , Li T. , Wang T. , Zhao Y. , Zhang X. , Xu H. , Liu Z. , Liu J. , Lin L. , Xie H. , Feng L. , Wang F. , Zhu F. , Hao J. , Quan S. , Liu K. , Huang S. 
Published: November 2024

High-brightness megahertz-rate electron beams are highly desired for cutting-edge applications in many areas of science. Photocathode electron guns capable of generating such beams with low dark current remain to be a challenging field. In this paper, we report breakthroughs with a hybrid gun combining a direct-current gap and a superconducting radio-frequency (SRF) cavity. The gun, employing K2CsSb photocathodes driven by a green laser, delivers a few MeV electron beam at 1 and 81.25 MHz rates with an average current up to 3 mA and a dark current several orders lower than current normal conducting continuous-wave guns. Emittance compensation and multipole field corrections have been applied to achieve a high-quality beam. The achievement will inspire the development of high-brightness SRF guns and promote megahertz-rate beam applications including coherent linac light sources and ultrafast electron diffraction/microscopy.

Published by the American Physical Society
2024
High-performance work systems and employee voice behavior: a sequential mediation analysis
Authors: Abou-Al-Ross Sami A. , Abualigah Ahmad , Barbar Julia , Kundi Yasir Mansoor , Dahleez Khalid Abed 
Published: November 2024

PurposeBased on ability-motivation-opportunity, social exchange, and job demands-resources theories, this research aims to investigate how high-performance work systems (HPWS) are linked to voice behavior through sequential mediating effects of psychological meaningfulness and work engagement in a Middle Eastern context.Design/methodology/approachStructural equation modeling was employed to analyze data obtained from a sample of 538 nurses working in various hospitals located in Palestine.FindingsThe findings show a positive association between HPWS and voice behavior. In addition, the study findings support the sequential mediation of psychological meaningfulness and work engagement on the association between HPWS and voice behavior.Originality/valueThis study is one of the few examining the effect of HPWS on individual-level outcomes in the context of the Middle East. More specifically, our study is the first to test the association between HPWS and voice behavior, especially in this under-researched context. It is also the first to explore the influence of HPWS on voice behavior through sequential mediating pathways of psychological meaningfulness and work engagement.
Role of cognition in mediating parental knowledge and support as precipitants of early adolescent delinquency escalation: partial replication and extension
Authors: Walters Glenn D. 
Published: November 2024

Purpose
The purpose of this study was to determine whether cognitive factors mediate the relationship between parental knowledge/support and delinquency escalation.

Design/methodology/approach
Using data from early adolescent youth enrolled in the Gang Resistance Education and Training (GREAT) study, two analyses were performed. The first analysis cross-lagged parental knowledge and cognitive impulsivity as predictors of delinquency escalation and the second analysis cross-lagged parental support and moral neutralization as predictors of delinquency escalation.

Findings
In both analyses, the indirect effect of a change in parenting on delinquency escalation via a change in cognition attained significance, whereas the indirect effect of a change in cognition on delinquency escalation via a change in parenting did not. In neither case did the direct effect of parenting on delinquency achieve significance.

Research limitations/implications
This study was limited, however, by exclusive reliance on self-report measures to assess all variables in this study and the use of explicit rather than implicit measures of cognitive impulsivity and moral neutralization.

Practical implications
The practical implications of these results are that they point to ways in which improved parenting can lead to crime deceleration; reduced cognitive impulsivity and moral neutralization can lead to crime deceleration.

Social implications
These results imply that social variables like parental knowledge and support stimulate a change in cognition as part of the process by which delinquency escalates during early adolescence.

Originality/value
The unique contribution this study makes to the field is that it highlights the role antisocial cognition plays in mediating between social factors and delinquency as part of the crime acceleration process that often occurs in early adolescence.
Genetic Characterization of a Novel Retron Element Isolated from Vibrio mimicus
Authors: Caigoy Jant Cres , Shimamoto Toshi , Ishida Yojiro , Ahmed Ashraf M. , Miyoshi Shin‐ichi , Shimamoto Tadashi 
Published: November 2024

ABSTRACTBacterial reverse transcriptase coding gene (RT) is essential for the production of a small satellite DNA‐RNA complex called multicopy single‐stranded DNA (msDNA). In this study, we found a novel retron, retron‐Vmi1 (Vm85) from Vibrio mimicus. The retron is comprised of the msr‐msd region, orf323, and the ret gene, a genetic organization similar to Salmonella's retron‐Sen2 (St85). The protein sequence of the RNA‐directed DNA polymerase (RT‐Vmi1) is highly homologous to the RTs of Vibrio metoecus, Vibrio parahaemolyticus, and Vibrio vulnificus. Phylogenetic and protein sequence similarity analysis of retron‐Vmi1 ORF323 and RT revealed a close relatedness to retron‐Sen2. We found that retron‐Vmi1 was inserted in the dusA gene, similar to the insertion of the retron‐Vpa1 (Vp96) of V. parahaemolyticus AQ3354, suggesting that retrons can be transferred via the tRNA gene. These results are the first convincing evidence that retron is moving across species. The neighboring genes of retron‐Vmi1 shared high homology with the genetic environment of V. parahaemolyticus and V. vulnificus retrons. We also found two junction points within the retron‐Vmi1 and the dusA gene suggesting that retron‐Vmi1 was inserted into this site in a two‐step manner.
A quiet convergence: The 2022 full‐scale Russian invasion of Ukraine and Italian Parties' positions on the European Union
Authors: Capati Andrea , Trastulli Federico 
Published: November 2024

AbstractWe investigate the impact of the 2022 Russian invasion of Ukraine on domestic party competition over the European Union (EU) in its immediate aftermath, with a focus on the case of Italy. Drawing on a discursive neo‐functionalist framework, we perform OLS regression analysis on a dataset of more than 10,000 Facebook posts retrieved via CrowdTangle. Our findings show that Italian parties converged towards supportive EU positions following the full‐scale Russian invasion due to a decrease in anti‐EU claims. Furthermore, the degree of convergence of individual parties varied depending on their ideological orientation. While extreme left‐wing and green parties significantly decreased their discursive opposition to the EU, anti‐Europeanism did not abate in radical right parties, which remained consistent in their Eurosceptic stances throughout the analysed timeframe. Compared to COVID‐19, the different surrounding international context and policy fields involved in this crisis are associated with different party reactions vis‐à‐vis the EU.
“Ineffective breastfeeding (00104)” and “Readiness for enhanced breastfeeding (00106)”: Clinical validation
Authors: de Godoy Natália Aparecida , Emidio Suellen Cristina Dias , Jones Dorothy A. , Carmona Elenice Valentim 
Published: November 2024

AbstractPurposeTo clinically validate the NANDA International (NANDA‐I), nursing diagnoses (ND) of “Ineffective breastfeeding (00104)” and “Readiness for enhanced breastfeeding (00106)” in postpartum women and their infants, and to determine the sensitivity, specificity, and predictive value of their elements.MethodsCross‐sectional study was conducted from June 2023 to April 2024. Recruitment happened at a public teaching hospital, approaching dyads from the neonatology outpatient clinic and the rooming‐in. The researcher applied the tool, assessed the dyad and medical records. Statistical analysis was based on accuracy measures and inferential statistics. The study was approved by the Ethics Committee.FindingsThe sample comprised 205 Brazilian dyads: 57 (27.80%) presented “Ineffective breastfeeding (00104),” whereas 203 (99.02%) presented the “Readiness for enhanced breastfeeding (00106).” “Unsustained sucking on the breast” was the most sensitive defining characteristic (DC) for the “Ineffective breastfeeding (00104).” All the DCs of “Readiness for enhanced breastfeeding (00106)” showed relevant sensitivity.ConclusionsFindings validated the presence of NANDA‐I ND “Ineffective breastfeeding (00104)” and “Readiness for enhanced breastfeeding (00106)” in a sample of dyads, demonstrating accuracy in such population.Implications for nursing practiceThis is an essential step to raise the level of evidence of the studied ND from NANDA‐I terminology. Furthermore, this study has potential to contribute to improving nurse's clinical reasoning on breastfeeding, as well as to highlight that women can present simultaneously the two diagnoses, so naming them is useful to identify mothers more likely to breastfeed and those who will need more differentiated interventions.
Ineffective overweight self‐management: A study of diagnostic accuracy and etiological relationships
Authors: Carino Ana Carolina Costa , Fernandes Renata Marinho , Dantas Juliane Rangel , Martins Cláudio César Guimarães , de Araújo Annaiza Freitas Lopes , Fernandes Maria Isabel da Conceição Dias , Lopes Marcos Venícios de Oliveira , Lira Ana Luisa Brandão de Carvalho 
Published: November 2024

AbstractPurposeTo evaluate the accuracy of clinical indicators and etiological factors associated with the nursing diagnosis of ineffective overweight self‐management among university students.MethodA diagnostic accuracy study with a cross‐sectional design was conducted with 108 university students. A latent class analysis model with random effects was used to establish sensitivity and specificity measures of clinical indicators, estimate the prevalence of the diagnosis, and calculate posterior probabilities for the diagnosis. Odds ratios for etiological factors were calculated using univariate logistic regression. The study was approved by the Research Ethics Committee of the responsible institution.ResultsThe nursing diagnosis of ineffective overweight self‐management had an estimated prevalence of 38.1% in the sample. Sensitive clinical indicators included excessive calorie consumption (0.9514) and dissatisfaction with body image (0.8834). Specific indicators included negative self‐perception of health (0.9999), inadequate eating behavior (0.9990), and impaired emotional health (0.8273). A personal history of overweight (OR = 2.78; p‐value = 0.018) increased the likelihood of developing ineffective overweight self‐management by approximately two and a half times.ConclusionIneffective overweight self‐management is prevalent among university students and is characterized by two sensitive clinical indicators, three specific indicators, and a population at risk that increases the likelihood of this diagnosis.Implications for nursing practiceAccurate clinical indicators and the identification of etiological factors for the diagnosis of ineffective overweight self‐management support nurses’ clinical reasoning in practice. Moreover, verifying accurate components for diagnosing ineffective overweight self‐management contributes to the development of interventions aimed at promoting the health of university students.
The soil Mycobacterium sp. promotes health and longevity through different bacteria‐derived molecules in Caenorhabditis elegans
Authors: Liu Limeng , Hao Xusheng , Bai Yang , Tian Ye 
Published: November 2024

AbstractCommensal bacteria and their derivatives hold significant promise as therapeutic interventions to delay aging. However, with the diverse nature of the soil microbiome and the long lifespan of mammalian models, the exploration of the influence of soil bacteria and bacteria‐derived molecules on host aging remains limited. We conducted a lifespan screening in Caenorhabditis elegans using plant root bacterial collection. Our screening identified 8 genera of bacterial isolates capable of extending lifespan, with Mycobacterium sp. Root265 exhibits the most pronounced effect on lifespan extension. Biochemical analysis revealed two specific molecules derived from Root265, polysaccharides (PSs) and arabinogalactan peptidoglycan (AGP), responsible for lifespan extension via daf‐16‐dependent and ‐independent pathways, respectively. Notably, AGP exhibited a unique ability to enhance protein homeostasis effectively. Moreover, polar lipids originating from Root265 were found to extend lifespan while mitigating age‐related BAS‐1 decline in neurons. Intriguingly, even brief exposures to these bioactive compounds were sufficient to achieve the lifespan‐promoting effects. We found diverse beneficial bacteria and anti‐aging active compounds from soil bacteria. These findings highlight the potential of exploring bacterial derivatives as therapies targeting aging without the constraints associated with direct microbial interventions.
Clinical outcomes in febrile children with a positive urine culture without pyuria: Implications for antibiotics
Authors: Miyake Rei , Hataya Hiroshi 
Published: November 2024

AbstractAimThis study aimed to investigate whether antibiotics should be administered to patients aged <2 years with a positive urine culture without pyuria by identifying their clinical characteristics and outcomes in the absence of antibiotic treatment.MethodsThis retrospective, observational study included children aged <2 years with positive findings on a culture of urine obtained using a catheter in the paediatric emergency department between 2016 and 2021. The primary outcome was the spontaneous resolution of fever without antibiotics in patients with a positive urine culture without pyuria. The clinical characteristics of the patients with and without pyuria were also compared.ResultsOf 391 patients, 86 (22%) were negative for pyuria. Of these, 63 (73%) received no antibiotics, and 56 (89%) had spontaneous defervescence and no symptom recurrence. The patients without pyuria had a higher proportion of other possible causes of fever, lower inflammatory markers, fewer cultured bacteria, and a higher proportion of multiple, bacterial species in their culture than the patients with pyuria.ConclusionsMost of the patients with a positive urine culture without pyuria may not require antibiotics if their fever resolves spontaneously.
Impact of antibiotic duration and type on short‐ and long‐term outcomes in very‐low‐birthweight infants
Authors: Loe Marcus Wing Choy , Sultana Rehena , Goh Guan Lin , Lim Selina Wan Xuan , Yeo Kee Thai 
Published: November 2024

AbstractAimTo determine the association between the duration and types of antibiotic exposure and the occurrence of short‐ and long‐term outcomes among preterm, very‐low‐birthweight (VLBW) infants.MethodsRetrospective cohort study of VLBW infants born <32 weeks gestation between January 2017–December 2021. Association between antibiotic exposure and the occurrence of death and/or major morbidities, and neurodevelopmental impairment (NDI) at 18–24 months corrected age, was evaluated.ResultsA total of 728 infants were included—median gestation 28 weeks (IQR 26, 30) and median birthweight 1070g (IQR 850, 1300). Compared to no antibiotics, antibiotics exposure >3 days was significantly associated with increased risk of mortality and/or bronchopulmonary dysplasia (BPD)—adjusted odds ratio (AOR) 2.0 (95% CI 1.1–3.8; p=0.03), 3.5 (95% CI 1.6–7.8; p=0.01) and 3.8 (95% CI 1.6‐9.0; p<0.001) corresponding to antibiotic exposure of 4‐7 days, 8–14 and >14 days, respectively. Additional exposure to broad‐spectrum antibiotics was associated with AOR of 3.2 (95% CI 1.6–6.5; p<0.01) for death and/or BPD. There was no significant association between antibiotic exposure and NDI.ConclusionInfants who received >3 days of antibiotics and/or additional broad‐spectrum antibiotics had significantly increased odds of death and/or BPD compared to no exposure. Antibiotic exposure was not significantly associated with NDI in our population.
Nature, predictors, and outcomes of Nurses' trajectories of harmonious and obsessive passion
Authors: Cheyroux Pierre , Morin Alexandre J. S. , Colombat Philippe , Gillet Nicolas 
Published: November 2024

AbstractThis study sought to achieve a dynamic person‐centered understanding of the various harmonious and obsessive work passion trajectories observed among a sample of nurses, as well as the connections between these two types of work passion trajectories. Moreover, it sought to document the predictive role of workload, unfairness, harassment, and supervisor support in relation to these harmonious and obsessive passion trajectories, as well as the implications of these trajectories for a variety of outcomes related to attitude (i.e., turnover intention), psychological health (i.e., perceived psychological health and work fatigue), and behaviors (i.e., work performance, presenteeism, and absenteeism). A sample of 622 nurses was surveyed six times over a period of five months. Our results revealed that harmonious and obsessive passion trajectories matched five primary profiles, similar across the two types of work passion. Workload, unfairness, harassment, and supervisor support were associated with these trajectories in a way that mainly supported our expectations. Trajectories characterized by higher levels of harmonious passion and lower levels of obsessive passion were associated with higher levels of perceived psychological health and work performance, and with lower levels of work fatigue, turnover intention, presenteeism, and absenteeism. Conversely, trajectories characterized by lower levels of harmonious passion and higher levels of obsessive passion were associated with the most negative outcomes.
Influence of Metal Artifact Reduction Tool of Two Cone Beam CT on the Detection of Bone Graft Loss Around Titanium and Zirconium Implants—An Ex Vivo Diagnostic Accuracy Study
Authors: Chang Michelle , Felizardo Henrique Mateus Alves , Oliveira‐Santos Christiano , Gaêta‐Araujo Hugo 
Published: November 2024

ABSTRACTObjectiveThe objective of this study is to investigate the influence of metal artifact reduction (MAR) on two cone beam computed tomography (CBCT) units in the evaluation of bone graft loss adjacent to titanium (Ti) and zirconium (Zr) implants.Material and MethodsTwelve Ti and twelve Zr implants were placed in the posterior region of dry human mandibles. Bone graft was applied to the level of the cover screw. Bone graft loss was simulated in half of the sample (6 Ti and 6 Zr) by removing the graft material up to the third implant thread on the buccal surface. CBCT images were acquired on two units, varying the application of MAR (OP300—off and on; Eagle 3D—standard, intermediate, and extreme). The images were assessed by five evaluators that scored the presence of graft loss according to a 5‐point scale. The diagnostic values were calculated and compared by non‐parametric tests with a significance level of 5%.ResultsHigher diagnostic values were achieved with MAR activated in the OP300 unit, for Ti and Zr (p < 0.05). On the Eagle 3D unit, MAR in extreme mode resulted in lower diagnostic values for both types of implants (p < 0.05). The diagnostic values of Ti implants were higher than Zr implants (p < 0.05).ConclusionThe application of MAR influences the diagnosis of bone graft loss adjacent to Ti and Zr dental implants. However, the extreme mode of MAR in the Eagle 3D unit can impair the diagnostic task in both types of implants and should be avoided.