bluuebunny/crossref_metadata_2025_split huggingface dataset:
A putative novel role forEip74EFin male reproduction in promoting sperm elongation at the cost of male fecundity
Authors: Chebbo Sharif , Josway Sarah , Belote John M. , Manier Mollie K. 
Published: September 2019

ABSTRACTSpermatozoa are the most morphologically variable cell type, yet little is known about genes controlling natural variation in sperm shape.Drosophilafruit flies have the longest sperm known, which are evolving under postcopulatory sexual selection, driven by sperm competition and cryptic female choice. Long sperm outcompete short sperm but primarily when females have a long seminal receptacle (SR), the primary sperm storage organ. Thus, selection on sperm length is mediated by SR length, and the two traits are coevolving across theDrosophilalineage, driven by a genetic correlation and fitness advantage of long sperm and long SR genotypes in both males and females.Ecdysone induced protein 74EF(Eip74EF) is expressed during post-meiotic stages of spermatogenesis, when spermatid elongation occurs, and we found that it is rapidly evolving under positive selection inDrosophila. Hypomorphic knockout of theE74Aisoform leads to shorter sperm but does not affect SR length, suggesting that E74A may be involved in promoting spermatid elongation but is not a genetic driver of male-female coevolution. We also found thatE74Aknockout has opposing effects on fecundity in males and females, with an increase in fecundity for males but a decrease in females, consistent with its documented role in oocyte maturation. Our results suggest a novel function ofEip74EFin spermatogenesis and demonstrates that this gene influences both male and female reproductive success. We speculate on possible roles for E74A in spermatogenesis and male reproductive success.RESEARCH HIGHLIGHTSEip74EFpromotes oocyte maturation inDrosophila. We found evidence that it also promotes sperm elongation in males, but at a cost to male fecundity. Mutant males have shorter sperm but have higher reproductive success, while females have reduced fecundity.
De novo assembly of a Tibetan genome and identification of novel structural variants associated with high altitude adaptation
Authors: Ouzhuluobu  , He Yaoxi , Lou Haiyi , Cui Chaoying , Deng Lian , Gao Yang , Zheng Wangshan , Guo Yongbo , Wang Xiaoji , Ning Zhilin , Li Jun , Li Bin , Bai Caijuan , Baimakangzhuo  , Gonggalanzi  , Dejiquzong  , Bianba  , Duojizhuoma  , Liu Shiming , Wu Tianyi , Xu Shuhua , Qi Xuebin , Su Bing 
Published: September 2019

AbstractStructural variants (SVs) may play important roles in human adaption to extreme environments such as high altitude but have been under-investigated. Here, combining long-read sequencing with multiple scaffolding techniques, we assembled a high-quality Tibetan genome (ZF1), with a contig N50 length of 24.57 mega-base pairs (Mb) and a scaffold N50 length of 58.80 Mb. The ZF1 assembly filled 80 remaining N-gaps (0.25 Mb in total length) in the reference human genome (GRCh38). Markedly, we detected 17,900 SVs, among which the ZF1-specific SVs are enriched in GTPase activity that is required for activation of the hypoxic pathway. Further population analysis uncovered a 163-bp intronic deletion in the MKL1 gene showing large divergence between highland Tibetans and lowland Han Chinese. This deletion is significantly associated with lower systolic pulmonary arterial pressure, one of the key adaptive physiological traits in Tibetans. Moreover, with the use of the high quality de novo assembly, we observed a much higher rate of genome-wide archaic hominid (Altai Neanderthal and Denisovan) shared non-reference sequences in ZF1 (1.32%-1.53%) compared to other East Asian genomes (0.70%-0.98%), reflecting a unique genomic composition of Tibetans. One such archaic-hominid shared sequence, a 662-bp intronic insertion in the SCUBE2 gene, is enriched and associated with better lung function (the FEV1/FVC ratio) in Tibetans. Collectively, we generated the first high-resolution Tibetan reference genome, and the identified SVs may serve as valuable resources for future evolutionary and medical studies.
MetaLab 2.0 enables accurate post-translational modifications profiling in metaproteomics
Authors: Cheng Kai , Ning Zhibin , Zhang Xu , Li Leyuan , Liao Bo , Mayne Janice , Figeys Daniel 
Published: September 2019

AbstractStudying the structure and function of microbiomes is an emerging research field. Metaproteomic approaches focusing on the characterization of expressed proteins and post-translational modifications (PTMs) provide a deeper understanding of microbial communities. Previous research has highlighted the value of examining microbiome-wide protein expression in studying the roles of the microbiome in human diseases. Nevertheless, the regulation of protein functions in complex microbiomes remains under-explored. This is mainly due to the lack of efficient bioinformatics tools to identify and quantify PTMs in the microbiome. We have developed a comprehensive software termed MetaLab for the data analysis of metaproteomic datasets. Here we build an open search workflow within MetaLab for unbiased identification and quantification of PTMs from microbiome samples. This bioinformatics platform provides information about proteins, PTMs, taxa, functions, and pathways of microbial communities. The performance of the workflow was evaluated using conventional proteomics, metaproteomics from mouse and human gut microbiomes, and modification-specific enriched datasets. Superior accuracy and sensitivity were obtained simultaneously by using our method comparing with the traditional closed search strategy.
Integrative model to coordinate the oligomerization and aggregation mechanisms of CCL5
Authors: Chen Yi-Chen , Chen Siou-Pei , Li Jin-Ye , Chen Pei-Chun , Lee Yi-Zong , Li Kun-Mou , Zarivach Raz , Sun Yuh-Ju , Sue Shih-Che 
Published: September 2019

AbstractThe CC-type chemokine ligand 5 (CCL5) is involved in the pathogenesis of many inflammatory conditions. The oligomerization and aggregation of CCL5 are considered to be responsible for its inflammatory properties. The CC-type dimer acts as the basic unit to constitute the oligomer. However, the structural basis of CCL5 oligomerization remains controversial. In this study, NMR and biophysical analyses proposed evidence that no single dimer-dimer interaction dominates in the oligomerization process of CCL5. CCL5 could oligomerize alternatively through two different interactions, E66-K25 and E66-R44/K45. In addition, a newly determined trimer structure reported an interfacial interaction through the N-terminal12FAY14sequence. The interaction contributes to aggregation and precipitation. In accordance with the observations, an integrative model explains the CCL5 oligomerization and aggregation process. CCL5 assembly consists of two types of dimer-dimer interactions and one aggregation mechanism. For full-length CCL5, the molecular accumulation triggers oligomerization through the E66-K25 interaction, and the12FAY14interaction acts as a secondary effect to derive aggregation. The E66-R44/K45 interaction dominates in CCL5 N-terminal truncations. The interaction would lead to filament-like formation in solution.
Corrosion behavior of X80 steel under disbond coating interfered with AC
Authors: Ding Qingmiao , Li Zili , Shen Tao , Cui Gan 
Published: September 2019

Purpose
This paper aims to research the corrosion behavior of the metal under the disbonded coatings interfered with AC through electrochemical method.

Design/methodology/approach
The corrosion behavior of the metal under disbond coating interfered with alternate stray current (AC) was studied by electrochemical methods using the rectangular coating disbonded simulator. The obtained data from electrode potential test, electrochemical impedance spectroscopy (EIS) and polarization curves in simulated soil solution indicated that under the natural corrosion condition, the self-corrosion potential and the corrosion current density of the metal at different depths under disbond coating had obviously changed if there was AC interference.

Findings
The self-corrosion potential of the metal at the same depths under disbond coating shifted negatively with the rising of the AC voltage. Under the condition of cathode polarization, there was still obvious potential gradient with the extension of the deep peeling of the coating gap, and the corrosion current density of the test points was minimum, and the protection effect was best when the cathode protection potential was −1.0 V. When the metal was applied with over-protection, the corrosion rate of the metal increased as AC stray current flowing through it increased.

Originality/value
This paper used the rectangular aperture device to study the corrosion behavior of X80 steel under the disbonded coatings through electrochemical methods when the AC stray current interference voltage was 0V, 1V, 5V or 10V and the protection potential was 0V, −0.9V, −1.0V, −1.2V or −1.3V, respectively. There is great significance to the safe operation and long-term service of pipeline steel in soil environment.
An 8kW LLC resonant converter in plasma power supply based on SiC power devices for efficiency improvement
Authors: Wang Zhenmin , Fan Wenyan , Xie Fangxiang , Ye Chunxian 
Published: September 2019

Purpose
This paper aims to present an 8 kW LLC resonant converter designed for plasma power supply with higher efficiency and lighter structure. It presents how to solve the problems of large volume and weight, low performance and low efficiency of traditional plasma power supply.

Design/methodology/approach
At present, conventional silicon (Si) power devices’ switching performance is close to the theoretical limit determined by its material properties; the next-generation silicon carbide (SiC) power devices with outstanding advantages can be used to optimal design. This 8 kW LLC resonant converter prototype with silicon carbide (SiC) power devices with a modulated switching frequency ranges from 100  to 400 kHz.

Findings
The experimental results show that the topology, switching loss, rectifier loss, transformer loss and drive circuit of the full-bridge LLC silicon carbide (SiC) plasma power supply can be optimized.

Research limitations/implications
Due to the selected research object (plasma power supply), this study may have limited universality. The authors encourage the study of high frequency resonant converters for other applications such as argon arc welding.

Practical implications
This study provides a practical application for users to improve the quality of plasma welding.

Originality/value
The experimental results show that the full-bridge LLC silicon carbide (SiC) plasma power supply is preferred in operation under conditions of high frequency and high voltage. And its efficiency can reach 98%, making it lighter, more compact and more efficient than previous designs.
External career mentoring and mentor turnover intentions
Authors: Renn Robert W. , Steinbauer Robert , Huning Tobias Michael 
Published: September 2019

PurposeAlthough studies have improved understanding of the relation between external career mentoring and mentor work outcomes, an important question remains regarding whether this mentoring function influences mentor turnover intentions. The purpose of this paper is to investigate the impact of career mentoring outside the workplace on mentor turnover intentions.Design/methodology/approachData were collected from 101 working business professionals in the southeastern USA at two points in time who provided career mentoring to business student protégés in an eight-month university sponsored mentoring program.FindingsAs hypothesized, moderated mediation analysis indicated that amount of external career mentoring negatively related to mentor turnover intentions and that the indirect effect of external career mentoring on mentor turnover intentions via mentor work engagement was stronger when both mentor protégé satisfaction and meeting frequency were high vs low. A two-way interaction revealed that mentors reporting higher protégé satisfaction had lower turnover intentions when meeting frequency was high vs low.Originality/valueThe findings help clarify the external career mentoring and mentor turnover intentions relation and have valuable theoretical implications for research on the benefits external mentoring can provide mentors. They also have practical implications for using external mentoring to enhance mentor work engagement and reduce mentor turnover intentions.
Fast multi-objective design optimization of microwave and antenna structures using data-driven surrogates and domain segmentation
Authors: Koziel Slawomir , Bekasiewicz Adrian 
Published: September 2019

Purpose
The purpose of this paper is to investigate the strategies and algorithms for expedited design optimization of microwave and antenna structures in multi-objective setup.

Design/methodology/approach
Formulation of the multi-objective design problem-oriented toward execution of the population-based metaheuristic algorithm within the segmented search space is investigated. Described algorithmic framework exploits variable fidelity modeling, physics- and approximation-based representation of the structure and model correction techniques. The considered approach is suitable for handling various problems pertinent to the design of microwave and antenna structures. Numerical case studies are provided demonstrating the feasibility of the segmentation-based framework for the design of real-world structures in setups with two and three objectives.

Findings
Formulation of appropriate design problem enables identification of the search space region containing Pareto front, which can be further divided into a set of compartments characterized by small combined volume. Approximation model of each segment can be constructed using a small number of training samples and then optimized, at a negligible computational cost, using population-based metaheuristics. Introduction of segmentation mechanism to multi-objective design framework is important to facilitate low-cost optimization of many-parameter structures represented by numerically expensive computational models. Further reduction of the design cost can be achieved by enforcing equal-volumes of the search space segments.

Research limitations/implications
The study summarizes recent advances in low-cost multi-objective design of microwave and antenna structures. The investigated techniques exceed capabilities of conventional design approaches involving direct evaluation of physics-based models for determination of trade-offs between the design objectives, particularly in terms of reliability and reduction of the computational cost. Studies on the scalability of segmentation mechanism indicate that computational benefits of the approach decrease with the number of search space segments.

Originality/value
The proposed design framework proved useful for the rapid multi-objective design of microwave and antenna structures characterized by complex and multi-parameter topologies, which is extremely challenging when using conventional methods driven by population-based metaheuristics algorithms. To the authors knowledge, this is the first work that summarizes segmentation-based approaches to multi-objective optimization of microwave and antenna components.
Employees’ perceptions of human resource management practices and employee outcomes
Authors: Li Shaoheng , Rees Christopher J. , Branine Mohamed 
Published: September 2019

PurposeThe purpose of this paper is to examine the relationship between employees’ perceptions of HRM practices and two outcomes, namely, employee commitment and turnover intention (TI), in small- and medium-sized enterprises (SMEs) in mainland China.Design/methodology/approachThis paper adopts a quantitative approach based on a sample of 227 employees working in 24 SMEs in eastern and western China.FindingsEmployees’ perceptions of HRM practices, such as training and development, reward management and performance management, are significant predictors of employee commitment. A negative direct relationship is found between employees’ perceptions about the use of HRM practices and TIs.Research limitations/implicationsAlthough data were collected from two representative provinces of eastern and western China, the size of the sample may limit the generalisability of the findings to the wider region.Practical implicationsThe relationship between employees’ perceptions of HRM practices and employee outcomes in Chinese SMEs provides an effective way for SME owners and HR practitioners to generate desirable employee attitudes and behaviours, which, ultimately contribute to improving organisational performance.Originality/valueThis is an original paper which makes a contribution by helping to address the dearth of studies which have explored aspects of the effectiveness of HRM in SMEs in China. In contrast to the majority of China-focussed studies on this topic, it highlights HRM outcomes at the individual level rather than the organisational level. Further, the study involves SMEs in western China which is an under-explored region.
Numerical simulation of droplet dynamics on chemically heterogeneous surfaces by lattice Boltzmann method
Authors: Wang Xin , Xu Bo , Chen Z. 
Published: September 2019

Purpose
This paper aims to investigate spontaneous movement of single droplet on chemically heterogeneous surfaces induced by the net surface tension, using the improved three-dimensional (3D) lattice Boltzmann (LB) method.

Design/methodology/approach
D3Q19 Shan-Chen LB model is improved in this paper. Segmented particle distribution functions coupled with the P-R equation of state are introduced to maintain the higher accuracy and greater stability. In addition, exact difference method (EDM) is adopted to implement force term to predict the droplet deformation and dynamics.

Findings
The numerical results demonstrate that spontaneous movement of single droplet (=1.8 µm) along wedge-shaped tracks is driven by net surface tension. Advancing angle decreases instantaneously with time, while receding angle changes slightly first and then decreases rapidly. Wetting length is affected by vertex angle and wetting difference, whereas the final value is only dependent on the stronger wettability. Although the velocity of single droplet on wedge-shaped tracks can be increased by the larger vertex angle, it has a negative influence on the displacement. For the same wetting difference, vertex angle equal to 30º is an optimization strategy in this model. If the simulation length is extended enough, then the smaller vertex angle is beneficial for the droplet movement. In addition, a larger wetting difference is beneficial to spontaneous movement, which can speed up the droplet movement.

Originality/value
The proposed numerical model of droplet dynamics on chemically heterogeneous surfaces provides fundamental insights for the enhancement of drop-wise condensation heat transfer.
Multiple-relaxation-time lattice Boltzmann model for simulation of free convection in axisymmetric nanofluid-filled annulus-experimental and numerical observations
Authors: Sun Heng , Ross David 
Published: September 2019

Purpose
The MRT lattice Boltzmann simulation of natural convection in a confined environment is carried out. The flow and heat transfer during natural convection in a symmetrical annulus are studied.

Design/methodology/approach
The cavity is filled with TiO2-water nanofluid, and the thermal conductivity and dynamic viscosity of nanofluid are measured experimentally. The experimental data are utilized in the numerical simulations. The nanofluids are prepared at four different nanoparticle concentrations φ = 0, 0.1, 0.3 and 0.5. It is notable that the radial coordinate is used into the temperature distribution function. As a result, only one source term is required for the present lattice Boltzmann model. On the other hand, the macro cylindrical energy equation is exactly recovered using Chapman–Enskog analysis.

Findings
Influence of some main parameters including Rayleigh number in range of 103 to 106, solid volume fraction of nanofluid in range of 0 to 0.5 and four different aspect ratios on the the nanofluid flow (i.e. streamlines), heat transfer (i.e. temperature distribution and average Nusselt number) and entropy generation (i.e. total entropy generation and Bejan number) are presented, quantitatively and graphically. It is found that adding TiO2 nanoparticles to the base fluid has considerable positive effect on the heat transfer performance and entropy generation. In addition, the configuration of the annulus can be good controlling parameter on the heat transfer rate during natural convection.

Originality/value
The originality of this work is using of a modern numerical method to simulate the free convection and conducting experimental observations to calculate the thermo-physical properties of nanofluid. In addition, the numerical and experimental works are combined to provide accurate results.
Microbreweries, brewpubs and beerfirms in the Sicilian craft beer industry
Authors: Alfeo Vincenzo , Todaro Aldo , Migliore Giuseppina , Borsellino Valeria , Schimmenti Emanuele 
Published: September 2019

Purpose
This paper aims to illustrate the organisational and managing models characterising the craft beer producers in Sicily (Southern Italy) and the main issues of the provision of raw materials.

Design/methodology/approach
A direct survey involving the overall population of 41 craft breweries operating in Sicily in 2016 was carried out. Then 29 questionnaires were collected for exploratory analysis. A hierarchical cluster analysis was also performed out to group companies by similar structural, productive and economic features.

Findings
The findings of the survey showed a Sicilian craft beer industry characterised by a substantial dependence on the import of malts hops and yeasts and the limited use of local raw materials among brewers. Furthermore, the characteristics of the processing plants and the sales channels appear to influence the diversification of the products and the turnover levels of the Sicilian craft beer producers.

Originality/value
This is the first study describing the craft brewing industry in Sicily. The findings contribute to enrich the knowledge on the organisational models applied in the craft beer industry. In particular, the findings could contribute to shed light on some critical issues about the provision of raw materials, suggesting possible paths for the successful development of the craft beer industry in the region.
Chasing Frankenstein’s monster: information literacy in the black box society
Authors: Lloyd Annemaree 
Published: September 2019

Purpose
The purpose of this paper is to introduce and examine algorithmic culture and consider the implications of algorithms for information literacy practice. The questions for information literacy scholars and educators are how can one understand the impact of algorithms on agency and performativity, and how can one address and plan for it in their educational and instructional practices?

Design/methodology/approach
In this study, algorithmic culture and implications for information literacy are conceptualised from a sociocultural perspective.

Findings
To understand the multiplicity and entanglement of algorithmic culture in everyday lives requires information literacy practice that encourages deeper examination of the relationship among the epistemic views, practical usages and performative consequences of algorithmic culture. Without trying to conflate the role of the information sciences, this approach opens new avenues of research, teaching and more focused attention on information literacy as a sustainable practice.

Originality/value
The concept of algorithmic culture is introduced and explored in relation to information literacy and its literacies.