bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Profile of Students’ Scientific Inquiry Literacy Based on Scientific Inquiry Literacy Test (ScInqLiT)
Authors: Innatesari D K , Sajidan S , Sukarmin S 
Published: June 2019

Abstract
This study aimed to describe the profile of scientific inquiry literacy. This study used a descriptive method with engaging 205 students from three junior high schools in Kediri Regency. The sample selection used a purposive sampling technique. The used scientific inquiry literacy instrument referred to ScInqLiT (Scientific Inquiry Literacy Test) by Wenning [12]. The results showed that students’ achievements of scientific inquiry literacy were indicated in the indicators such as identifying and controlling variable (38,05%) with low category; recognizing and analyzing alternative explanations and models (42,93%) with enough category; drawing appropriate conclusions from evidence (60,98%) with enough category; understanding and analyzing data (48,62%) with enough category; constructing and interpreting graphs (38,70%) with low category; constructing hypotheses (39,15%) with low category; designing experimental procedures (61,63%) with high category; and identifying problems to be investigated (51,95%) with enough category. Thus, it can be concluded that students’ scientific inquiry literacy in Kediri was relatively low. Accordingly, for the further studies, they are recommended to enhance some indicators of students’ scientific inquiry literacy with applying appropriate learning methods and teaching materials.
Development of Practicum Instructions for Junior High School Students: Formalin Test Using Natural Indicators
Authors: Rahman N , Purwoko A A , Muntari  , Haifaturrahmah  
Published: June 2019

Abstract
The purpose of this study was to develop a formaldehyde test practicum instructions using natural indicators for the first high school students. This research method refers to the development procedure developed by Borg and Galls. The trial of 104 leaf and flower crown samples which were estimated to have potential as a natural indicator for formalin testing was carried out. Only 3 natural indicators can be used for drops and filter paper, namely flower: ribosa ruellia, hibiscus rosa-sinensis L and balsamina Impatiens L purpel. Based on the results of the expert assessment and the teacher showed that the practicum instructions were suitable for use by junior high school students to test formalin.
Physiologic Glycated-Bovine Serum Albumin Determination using Spectrum-UV
Authors: Khoirunnisa W , Nur M I , Widyarti S , Permana S , Sumitro S B 
Published: June 2019

Abstract

Albumin is not native phase, but it physiologically-binding with another compound which conclude the functions as transporter and scavenger. The general non-enzymatic reaction within proteins, which has a significant impact on their physical and functional properties by reducing sugar, known as glycation. The study investigated the effective composition to glycate the bovine serum albumin (BSA) by UV-spectrum. Five BSA concentrations (750, 500, 100, 10 and 1 mM) was prepared in PBS pH 7.4. The glycation carried out using glucose concentrations (2M, 1.5 M, 1 M, 500 mM, and 100 mM) before and after incubation for seven days. Depending on concentration, BSA 1 mM and 10 mM showed the best UV spectrum of protein that two peaks, 220 and 280 nm. Hence, the glycation by high concentration of glucose would be made a conformational change of BSA which is marked by the UV-spectrum of BSA configuration. Glucose 100 and 500 mM was effective to glycate BSA.
Bioethanol from Sargassum sp Using Acid Hydrolysis and Fermentation Method Using Microbial Association
Authors: Kusuma Wardani Anggraeni , Herrani Retno 
Published: June 2019

AbstractSargassumsp is one of the abundant seaweed in Indonesian seas and it has the potential to be used as a renewable alternative energy source. DrySargassumsp contains 58.23% carbohydrate that can be used as bioethanol through a fermentation process. This research aims to determine the effect of fermentation time on the amount of ethanol content produced fromSargassumsp fermentation.The type of this study was laboratory experiment. The experiment was conducted by using microbial association namelyZymomonas mobilis,Tape Yeast and Bread Yeast with variation of fermentation duration of 4 days, 5 days, 6 days, and 7 days. Glucose test is done by using the DNS method. The measurement of ethanol content is done by using Gas Chromatography. In the data analysis the researcher uses correlation regression statistic method. The result of the research shows that the best treatment was 6 days fermentation duration with the amount of ethanol 24.67% with the reducing sugar content 54,570 ppm. The longer the fermentation time the higher the ethanol content produced except on the 7th day showing decline. The results of data analysis showed that the difference in fermentation time to ethanol content produced had a correlation coefficient that was positive and there was no significant relationship.
The effect of molase and urea on the production of alkaline protease from indigenous Aspergillus flavus DUCC K225
Authors: Rukmi I , Mulyani N S , Pujiyanto S 
Published: June 2019

Abstract
Protease is one of industrial important enzyme which can be produced by microorganisms. including fungi. Alkaline protease had physiological and commercially important. this enzyme used in detergent industries as additives. The indigenous mold Aspergillus flavus DUCC K225 isolated from lime soil of Madura island demonstrate the ability in producing extracellular protease at pH 8. The aim of this study was to examined the production of alkaline protease from A. flavus K225 on Czapeks Dox modified liquid medium containing molase as C-source and urea as N-source. The study were done in submerged culture on rotary shaker. The production of alkaline protease were observed after 7 days incubation. by examined fungal biomass. protease activity. protein content and protease specific activity. The examination of enzyme activity conducted at pH 8.5 done on crude enzyme and ammonium phosphate fractions of 0-20%. 20-40%. 40-60%. 60-80% and 80-100%. The results showed that the fungal biomass gained was 14.27 g/L. while the highest protease specific activity found at 40-60% ammonium sulphate fractination (71.75 U/ml) and 4.2 purification fold.
Development of immunohistochemical triple staining method for the evaluation of different types of cell death in coronary thrombus
Authors: Pertiwi K R , de Boer O J , Gabriels P , Mackaaij C , van der Wal A 
Published: June 2019

Abstract
Immunohistochemistry is a powerful technique to identify the presence, distribution and extent of certain antigens at protein level in tissue specimen. Multiple immunostainings enable the detection, colocalization and comparison of several markers within one specimen. However, an optimization study is firstly needed to ensure the expression of multiple markers is clear and distinguishable. We formulated an optimized sequential triple immunostaining protocol which could identify the simultaneous presence of three types of cell death namely necrosis, apoptosis and etosis. These following antibodies were used: C-reactive protein (CRP, necrosis), (cleaved) Caspase-3 (Casp3, apoptosis) and citrullinated-Histone3 (CitH3, etosis). Several antigen retrieval methods, various concentration and order combinations of those antibodies as well as different combination color of chromogens were tested on coronary thrombus materials obtained from patients with acute myocardial infarction (AMI). The results showed that CRP (1:4000) visualized with 3,3’-diaminobenzidine (DAB) in brown, is better performed as the first staining, followed by CitH3 (1:8000) visualized with Perma Blue as the second staining and Casp3 (1:500) visualized with Perma Red as the last staining. In conclusion, we presented an immunohistochemical triple staining protocol to identify the comparative presence of different types of cell deaths: necrosis, apoptosis and etosis in coronary thrombus specimens.
Effect of Fermentation to Total Titrable Acids, Flavonoid and Antioxidant Activity of Butterfly Pea Kombucha
Authors: Christiani Dwiputri Maria , Lauda Feroniasanti Y.M. 
Published: June 2019

Abstract

Butterfly Pea (Clitoria ternatea L.) has been utilized as traditional medicine to cure many kind of diseases. Previous study indicated that Butterfly Pea contains bioactive compounds that can be used as antioxidant, such as flavonoid. Butterfly Pea can be used as alternative substance for kombucha. This research aims to study about the effect of fermentation length towards total titrable acids content, total flavonoid content and antioxidant activity of butterfly pea kombucha. This research was conducted from March until May 2018 at the Laboratory of Biology and Pharmacy Department, Sanata Dharma University. This research used Completely Randomized Design (CRD) with the treatment of six different fermentation length, which was 0 day, 4 days, 8 days, 12 days, 16 days and 20 days. Each data obtained from this research were analyzed using correlation and regression statistic test. The results of the test showed that the treatment of fermentation length gave significant and positive correlation towards total titrable acids content and total flavonoid content. It means that the longer of fermentation length leads to higher of total titrable acids content and total flavonoid content. Whereas, the statistic test of antioxidant activity data showed that there was no significant correlation between the treatment of fermentation length to the levels of antioxidant activity of Butterfly Pea kombucha.
Use of Mung Bean Sprout (Tauge) as Alternative Fungal Growth Medium
Authors: Ilmi M , Putri L K , Muhamad A A K , Cholishoh A , Ardiansyah S A 
Published: June 2019

Abstract
Growth media are essential in mycological studies. Currently, laboratories are using ready-to-use media which are commercially available. However, the prices of such media are high and are a burden on low-funded laboratories. Mung bean sprout (tauge) has been used as a cheap alternative growth medium since 1974, but study comparing performance of the medium with its commercial counterparts has not been reported. This study was done to compare the performances of tauge extract with commercial Potato Extract and Yeast Extract for growing yeast and filamentous fungi. We also endeavoured to optimise the composition of the alternative media by statistical analysis. The results show that the tauge extract medium gave a significantly higher growth rate of Saccharomyces cerevisiae and a significantly lower growth rate of Kluyveromyces marxianus compared to the commercial media. On the other hand, the growth rates of Aspergillus oryzae and Trichoderma viride on all media are not significantly different. The optimum composition of tauge extract media for S. cerevisiae are 9.6 and 6.8 % (w/v) of tauge and sugar, respectively. For A. oryzae, maximum growth is predicted when the medium contains 11.2 and 7.4 % (w/v) of tauge and sugar, respectively.
Developing enrichment module of polyploidy on shallot (Allium ascalonicum L.) for 12th grade students
Authors: Khikmah F F , Suratsih  
Published: June 2019

Abstract
The objectives of this research were to determine the effect immersion duration and concentration of colchicine on polyploidy of shallots (Allium ascalonicum L.), to develop enrichment module based on the study, and to assess the enrichment module feasibility based on assessment of material experts, media experts, biology teachers, and responses of students. The research was development research using ADDIE model limited to development stage. Data of the study was the description of chromosome counting. The module feasibility data was obtained from the assessment by the reviewers and the responses of the respondents. The module feasibility assessment data was processed into quantitative data. The aspects analysed include the assessment of appropriateness of the concept, feasibility of content, presentation, language, and graphic. The results showed that colchicine’s immersion duration and concentrations had effects on polyploidy of shallots. The results of the research were used to develop module of mutation learning based on 2013 curriculum. Module review from material experts showed that most of the module’s substances were appropriate. Module review from media experts and responses of students showed that the module was feasible, and from biology teachers was highly feasible. The overall assessment results showed that polyploidy module was eligible feasible as teaching material in enrichment program for 12th grade students.
Teacher’s Perception Towards Implementation of Service Learning Model during Teaching and Learning of Biology in Senior High Schools in Yogyakarta District
Authors: Ajeng Antasari Galuh , Subali Bambang 
Published: June 2019

Abstract
This study aimed to determine the teacher’s perception towards implementation of service learning model during teaching and learning of Biology in senior high schools based on the; the status of school, i.e. state Senior High School and private Senior High School. This study used survey method to collect data from a sample population of 46 Biology teachers in 24 senior high schools in Yogyakarta district, Yogyakarta special region province, Indonesia. Amongst these schools, 11 state schools and 13 private schools based on ownership status. Data collection techniques used was questionnaires and data analysis was done by using descriptive statistical analysis. The study results showed that 32 % teachers from state was searching information about service learning and understand it. However just 9.09 % that implement it in biology learning. 37.5 % teacher from privately owned schools was searching information about service learning and understand it and 12.5 % apply it ini biology learning. Both had a positive perception towards the implemention of service learning model on certain learning materials like disorders and diseases that attack the human body system.
The Teachers’ Experiences in Implementation of Project Based Learning during Teaching and Learning of Biology in Senior High Schools in Yogyakarta District
Authors: Baptist K J , Subali B 
Published: June 2019

Abstract
This study aimed to; (1) find out how often teachers use PjBL during the teaching and learning process of biology, (2) assess the teachers experiences during guiding students on aspects involved in project designing, project implementation, and presenting the project results, and (3) find out how often the students were guided by their teachers on the aspects of project designing, project implementation, and presenting the project result. The research used the qualitative methodology to study 21 biology teachers and 250 teachers from tenth and eleventh grade of 10 state senior high schools in Yogyakarta district between March and May of the academic year 2018 / 2019. The data obtained from teachers’ and students’ questionnaires were analyzed using SPSS version 23. The results showed that; (1) most biology teachers (71.4%) frequently use PjBL, (2) majority of the teachers, (59.4%) project designing, (76.9%) project implementation, and (56.4%) presenting of project results felt it relatively easy to guide students on the aspects of PjBL and (3) most students (67.6%) are often guided by their teachers on aspects involved in the project designing, project implementation, and presenting of project results.
Students’ Scientific Argumentation Skills Based on Differences in Academic Ability
Authors: Ika Noviyanti Nurul , Rosyadah Mukti Windy , Dahlia Yuliskurniawati Irma , Mahanal Susriyati , Zubaidah Siti 
Published: June 2019

Abstract
One of the skills students must possess in the 21st-century biology learning is scientific argumentation skills. Scientific argumentation skills are associated with a strong understanding of concepts and improved critical thinking skills and scientific literacy. The purpose of this study was to investigate the scientific argumentation skills of high school students with different academic abilities. The current study was designed as a survey which involved tenth graders from two public senior schools, SMAN 3 Malang and SMAN 7 Malang. The first served as the representative of students with high academic ability and the latter was appointed to represent the low achievers. The data were analyzed using an independent-samples t-test. The results showed that there were significant differences between the high and ability students’ scientific argumentation skills with a p-value of 0.003. Around 10.34% of the high achievers could perform level 1 argumentation skills, 74.41% of them were able to achieve level 2, and 17.24% of the students reported level 3 responses. Among the students with low academic ability, 12% had reached level 1 argumentation skills and 88% of them were only able to achieve level 2, indicating that no one (0%) could answer the test with level 3 responses. This condition suggests that it is important to implement an innovative learning.
Profile content and context of biology modules based analytical thinking skill on the material of the human reproductive system
Authors: Putri Kus Sundari Puput , Widoretno Sri , Ashadi  
Published: June 2019

Abstract
This research aims to analyze biology modules based on the analytical thinking skill on the material of the human reproductive system. Research procedure was conducted by making indicators are used to analyze the content and context of modules through observation of classroom learning activities. The results of the analysis are presented in the form of percentage graphs which show that: 1) the five modules display the aspect of attributing the lowest percentage, 2) content has a lower percentage than the context of modules.
Empirical Validity of Collaborative-Learning Student Worksheet on Ecology Material to Practice Student’s Scientific Literacy Skill of Tenth Grade Senior High School
Authors: Anfa Qurrotul , Rachmadiarti Fida , Winarsih  , Aminatun Tien 
Published: June 2019

Abstract
The demands of the 21st century emphasize the importance of cooperation and collaboration in problem solving. Besides that, the element of scientific literacy is also needed by students to have a comprehensive learning experience in aspects of social attitudes skills, cognitive and psychomotor. Therefore, a collaborative learning media in the form of worksheets is required. The purpose of this study was to create collaborative learning student worksheets that is proper empirically on ecology material. This study used 3D model of development; Define, Design and Develop. The instruments used for empirical validity included student collaborative observation activity sheets, student scientific process observation activity sheets, student learning outcomes completeness sheets and student response sheets. Empirically, the result of student collaborative activities was very proper with 93.48%, the activity of students’ scientific process was also proper with 85.15%, the completeness of student learning outcomes received 85.87% and the positive response of students got 84.44%.
The Analysis of Environmental Attitudes of Public Junior High School Students in Ngawi
Authors: Arifiantari R , Ashadi  , Dwiastuti S 
Published: June 2019

Abstract
Environmental attitudes need to be developed by the students in the 21st century due to the world’s worse condition nowadays. Environmental attitudes are defined as someone’s internal condition to be willing or unwilling to preserve and take care of the environment in order to keep it stable. Students who possess such attitudes make quite significant impacts on the environmental quality. Besides, environmental attitudes are able to increase problem solving and decision making especially on the environmental issues. The objective of this research is to analyse environmental attitudes of public junior high school students in Ngawi. The participants are 7 graders of public junior high schools in Ngawi. The data are collected by distributing questionnaire concerning environmental attitudes based on NEP by Dunlap. The result indicates that the percentage of students categorized as pro is 38.33%, ones categorized as middle is 60.83%, and ones categorized as anti is 0.83%. Based on the result, it is concluded that the majority of the environmental attitudes of public junior high school students in Ngawi are categorized as middle. It becomes a serious concern to create better environmental quality.
Development of Assessment Instruments In Measuring Critical Thinking Skills of Senior High School Participants of Biology Subject
Authors: Hidayat S , Fadillah E.N. 
Published: June 2019

Abstract
The purposes of this study are to determine the instrument assessment appropriateness in measuring critical thinking skills and understand the level of critical thinking skills at 10th-grade senior high school participants of biology subjects at Palembang. This study is about research and development (R & D). The instrument of development model used is a 4-D development model from Thiagarajan, namely; define, design, develop, and disseminate. Aiken’s V qualitative and quantitative analysis by using QUEST program was used in this study. The results showed that; 1) Biological assessment instruments used in developing critical thinking skills of senior high school participants of the 10th class of biology subject are worth to be reviewed in terms of test standard characteristics; and 2) the percentage of critical thinking skills mastery level on interpretation indicators: 46.78%; 44.86% analysis; 41.15% evaluation; 49.12% inference; 47.50% explanation; and self-regulation 54.27%.
A Preliminary Study of Students Initial Creative Thinking Skills in Surakarta High School
Authors: Riani U Y , Nurcahyo H 
Published: June 2019

Abstract
Often founded more emphasis on memorizing the educational word that makes high level thinking ability of the students seldom trained. The 2013 Curriculum implemented higher-order thinking that encourage students to think critically, logically, reflective and creative. This study aims to describe the questionnaire of preliminary study and creative thinking skills of high school students in Surakarta. This research’s type is descriptive qualitative. Sampling techniques used a Purposive Sampling based on the location of the school and the category of schools. Test instruments of students’ creative thinking are provided towards four school in Surakarta. Data obtained from questionnaire of preliminary study and creative thinking skills instrument of student`s creative thinking skills. The validity and reliability of the test instrument analyzed quantitatively using the Quest. Internal consistency value is 0.90, it means that the instrument has high reliability. The results of the preliminary study shows that the majority of learning methods used by teacher was discussion (31.9%), lecture (42.2%), and laboratory activities (7.1%). While, the results of the creative thinking skills is shows that the students had very less category (11.2%), had less category (73.9%), and had medium category (14.9%).