bluuebunny/crossref_metadata_2025_split huggingface dataset:
Timing is Everything: Using a Phased Toolkit Approach in Large-Scale Education
Authors: Buckner Jessica B. , Kirkman Allyson , Tripp Heather , Fencl Jennifer 
Published: December 2024

Purpose
This article aims to share an innovative way to use virtual toolkits in large-scale education.

Description of Program
An incredible number of educational needs exist at any time in the hospital setting. Sharing information with hundreds of nurses in different departments or campuses is a vast undertaking for the clinical nurse specialist (CNS). At times, the material required for a single educational endeavor can be substantial, presenting another challenge for the CNS. Based on principles from Cognitive Load Theory and microlearning, a toolkit methodology allows in-depth education to be broken down into manageable portions for leaders, CNSs, and/or nursing professional development practitioners to effectively share with staff. The toolkit educational strategy utilizes calendar reminders with educational flyers, links, and content attached, making education accessible and easy to share.

Outcome
This method is an effective way to educate staff without requiring traditional class time.

Conclusion
CNSs, nursing professional development practitioners, and leaders alike can use the toolkit strategy for rolling out large initiatives in smaller portions over time, which can lead to better uptake and retention of information.
MR-Guided PET Reconstruction
Authors: Huellner Martin W. , Maurer Alexander , Spangler-Bickell Matthew 
Published: December 2024

Abstract
We report a case of a 33-year-old man with epilepsy and equivocal EEG, MRI signs of mesiotemporal sclerosis, and nondiagnostic standard FDG-PET imaging. The patient underwent repeat FDG-PET/MRI to clarify the sidedness of the epileptogenic focus and to confirm the suspected MTS. The standard PET reconstruction using block sequential regularized expectation maximization failed to provide evidence of a clear epileptogenic focus. However, using MR-guided PET reconstruction, circumscribed hypometabolism was observed in the right-sided entorhinal cortex, compatible with the epileptogenic focus. The MR-guided PET reconstruction provided significantly improved gray/white matter differentiation, enhancing confidence in imaging interpretation.
Evaluation of Situs Inversus Combined With Plastic Surgery–Related Malformations in a Chinese Clinic Population
Authors: Jia Ying , Zhang Ziming , Gao Shangjie , Jiang Fengli , Wang Yuhao , Su Xueshang , Zhuang Jun , Li Jingjing , Hu Jintian , Zhan Sien 
Published: December 2024

Background
Situs inversus is a congenital malformation that occurs in one or multiple organs simultaneously and can be accompanied by malformations in other body parts. We analyzed the prevalence and phenotype of patients with situs inversus and comorbidities associated with other plastic surgery–related malformations to enhance the knowledge of its related disorders and facilitate treatment.

Methods
We recruited patients with situs inversus who were seen at our institution from February 2015 to July 2023. They underwent ultrasound and physical examinations to investigate deformity in other body parts. Fisher's exact test was used to analyze the laterality between hemifacial microsomia and situs inversus type.

Results
All 14 patients with situs inversus had plastic surgery–related malformations—three congenital and three developmental. Nine (65%) patients had hemifacial microsomia (right side, n = 8; bilateral, n = 1). The laterality of hemifacial microsomia was associated with the situs inversus type, with a significant difference (P < 0.05), and hemifacial microsomia occurred ipsilaterally in patients with right-sided hearts.

Conclusions
Early situs inversus diagnosis is clinically important for patients and plastic surgeons. Further studies on anomalies associated with situs inversus will improve our understanding of their etiology.
Comprehensive Validation of the German Version of the LIMB-Q
Authors: Besmens Inga S. , Watson Jennifer A. , Akyildiz Efe , Mundy Lily R. , Giovanoli Pietro , Calcagni Maurizio , Lindenblatt Nicole 
Published: December 2024

Objective
Lower extremity trauma significantly impacts patients' lives, requiring a nuanced approach to evaluate outcomes beyond clinical measures. The LIMB-Q, a patient-reported outcome measure (PROM), assesses the multifaceted impacts of lower extremity trauma, including physical and emotional dimensions, from the patient's perspective. This study validates the German version of the LIMB-Q.

Methods
We relied on the translation of the LIMB-Q that had followed the International Society for Pharmacoeconomics and Outcomes Research best practice guidelines. Sixty patients who had undergone reconstructive surgery or amputation of the lower limb completed the LIMB-Q, Lower Extremity Functional Scale (LEFS), and Short Form Health Survey (SF-36). Internal consistency of the German LIMB-Q was determined by Cronbach's alpha, and reliability was assessed using the intraclass correlation coefficient. Construct validity was analyzed using Pearson correlation coefficients between the LIMB-Q, LEFS, and SF-36.

Results
Validation involved 60 patients. Internal consistency in a test-retest subset of 5 patients was good to excellent (α: 0.891 to 0.965). The intraclass correlation coefficient for these scales ranged from 0.821 to 1. The LIMB-Q domains significantly correlated with the corresponding domains of the SF-36 and LEFS, confirming excellent construct validity.

Conclusions
The German version of the LIMB-Q is conceptually equivalent to the original English version. It is a reliable and valid PROM for assessing physical and psychological impairments in patients who have undergone lower extremity reconstructive surgery or amputation.
A Physician-Centered Craniofacial Asymmetry Index for the Severity of Plagiocephaly
Authors: Lee Chien-Han , Lin Ting-Hsuan , Chen Shih-Heng , Chen Meng-Tse , Chen Pin-Ru , Shih Albert J. , Lee Chang-Chun , Chou Pang-Yun 
Published: December 2024

Background
Plagiocephaly, wherein infants' head exhibits a diagonal asymmetry, is currently diagnosed based on physicians' subjective judgment. Discrepancies between physician and parent perspectives may result in dissatisfaction with treatment outcomes. This problem highlights the need for an objective assessment system aligning with physician-made clinical diagnoses.

Methods
Infant heads were modeled using 3-dimensional scanning techniques. We developed a craniofacial asymmetric index (CAI) based on 10 height planes of heads with varying weight. CAI and traditional craniofacial vault asymmetry index (CVAI) of 10 infants undergoing helmet therapy were compared with 11 craniofacial surgeons' judgment. The Pearson correlation coefficient and Bland-Altman plot were used to determine the correlations and agreement between physicians' judgment and the aforementioned assessment methods. The adjusted intraclass correlation coefficient was calculated to evaluate the reliability of between-physician agreement.

Results
All 10 infants were divided into the following 3 severity groups: severe, moderate, and mild groups based on craniofacial surgeons' judgment. Notably in CAI, front/back halves of skull and multiangular weighting factors were evaluated. The evaluation revealed perfect alignment in severity classification between the CAI and physicians' judgment, whereas both the CVAI score and MATLAB analysis show varying degrees of difference, 6 and 4 distinct results, respectively. Coefficients of the correlations of physician-assigned scores with the MATLAB analysis, CVAI score, and CAI score were 0.500, 0.833, and 1.000, respectively. Furthermore, Bland-Altman plots revealed the best agreement between CAI and physician-assigned scores.

Conclusions
CAI closely aligns with the subjective judgment of craniofacial surgeons' assessing the severity of plagiocephaly in infants.
Optimal Treatment for Post-Surgical Cicatricial Alopecia: Autologous Hair Transplantation?
Authors: Zhu Ling , Niu Yanchao , Chi Longjun , Zhang Jian , Wang Jing , Ren Ci 
Published: December 2024

Background and Rationale:
Scarring alopecia significantly impacts patients’ appearance and psychological well-being. Surgery is a common cause of scarring alopecia, making positive treatment crucial for patients’ overall health.

Objective:
To investigate the clinical efficacy of autologous follicular unit extraction (FUE) transplantation for postoperative secondary stable scarring alopecia and evaluate its effectiveness.

Methods:
A retrospective descriptive study was conducted, including 14 patients with postoperative secondary scarring alopecia. All patients underwent initial autologous FUE hair transplantation, and postoperative complications were monitored. Patient satisfaction surveys were also conducted.

Results:
Satisfaction assessments were performed for all 14 patients. Two patients (14.3%) reported being very satisfied, 6 patients expressed satisfaction (35.7%), 4 patients reported being somewhat satisfied (28.6%), and 3 patients were dissatisfied (21.4%). Aesthetic complications included low follicular density post-transplantation, uneven density, and unnatural appearance.

Conclusion:
Solely using a single FUE hair transplantation for treating postoperative secondary scarring alopecia has limited effectiveness and may not always meet expectations, potentially requiring multiple transplantations. Further research is necessary to guide and improve follicular transplantation survival rates to ensure surgical efficacy.
Rouvière’s Sulcus as a Landmark for a Safe Laparoscopic Cholecystectomy: An Interim Analysis of a Multicenter Cross-sectional Study on the Prevalence and Morphologic Type of Rouvière’s Sulcus in the Italian Population
Authors: Cirocchi Roberto , Properzi Luca , Matteucci Matteo , Artico Marco , Vettoretto Nereo , Desiderio Jacopo , Di Cintio Antonio , Di Nardo Domenico , Farinacci Federico , Gemini Alessandro , Guerci Lorenzo , Mazzetti Stefano , Ricci Francesco , Trastulli Stefano , Avenia Stefano , Boselli Carlo , Cirillo Bruno , Brachini Gioia , Fedeli Piergiorgio , Montori Giulia , Ursi Pietro , Iandoli Ruggero , Bergamini Carlo , Giordano Alessio , Santoro Alberto , Mingoli Andrea , Antipas Pavlos , Tebala Giovanni Domenico 
Published: December 2024

Background:
Laparoscopic cholecystectomy is the gold standard in the treatment of symptomatic gallstones. The large number of gallbladders removed every year is not fully consistent with the excessively high incidence of iatrogenic bile duct injury (IBDI). Several strategies have been suggested to reduce this risk. Among them, the use of extra biliary anatomic structures, such as the Rouvière’s sulcus, as a landmark to guide the surgeon during dissection has been proposed as a means to prevent IBDI. The main aim of the present paper is the evaluation of the prevalence of Rouvière’s sulcus (RS) and its anatomic variants in a given population.

Materials and Methods:
This observational, cross-sectional, and multicenter study has been conducted at the Department of Digestive and Emergency Surgery of the “Azienda Ospedaliera Santa Maria,” Terni (Italy), at the Department of Surgical Sciences of the “Azienda Ospedaliera Perugia,” Perugia (Italy) and at the Department of Emergency and Trauma Surgery of the “Policlinico Umberto I,” Rome (Italy). Intraoperative images of 111 patients undergoing laparoscopic cholecystectomy were analyzed to identify the presence and type of RS, according to the Singh-Prasad classification and the Dahmane classification.

Results:
RS was present in 93 (83.8%) patients. Singh-Prasad type 1A is present in 48.4% of patients, type 1B in 25.8%, type 2 in 12.9% and type 3 in 12.9%. Dahmane’s open type is present in 48.4% of patients and fused type in 51.6%.

Conclusion:
Due to its high prevalence, RS can be used as an anatomic landmark and probably reduces the incidence of IBDI during laparoscopic cholecystectomy.
Unraveling Hepatic and Caval Flow to Improve Surgical Planning and Assessment in Fontan Patients via Novel 4D Flow CMR and CFD Methods
Authors: Sahni Akshita , Kannojiya Vikas , St. Clair Nicole , Hoganson David M. , Hammer Peter E. , del Nido Pedro J. , Rathod Rahul H. , Govindarajan Vijay 
Published: December 2024

AbstractThe Fontan procedure, a palliative surgery for single-ventricle heart defects, creates a non-physiological circulation that often leads to complications such as pulmonary arteriovenous malformations (PAVMs), thrombosis, and energy loss. As the number of Fontan patients surviving into adulthood continues to grow, precise understanding and improved monitoring of patient-specific hemodynamics is critical for long-term management. Two key challenges hinder progress: 1) the lack of a reliable method to quantify hepatic flow distribution (HFD) from post-operative imaging, a critical determinant of outcomes, and 2) the absence of a validated, patient-specific computational fluid dynamics (CFD) workflow to guide surgical personalization.This study addresses these gaps by integrating a novel 4D Flow CMR-based particle tracking algorithm with an image-based CFD workflow to quantify and predict Fontan hemodynamics, including HFD, validated with in-vivo data. The 4D Flow-based particle tracking algorithm offers a precise, non-invasive tool for visualizing HFD, demonstrating excellent agreement with phase-contrast MRI (< 5% deviation). Patient-specific CFD models further predicted flow dynamics with high accuracy, validated against in vivo data (< 8% deviation).Using this integrated workflow, we uncovered uneven mixing between the inferior vena cava and hepatic blood flow, challenging assumptions of uniform mixing and highlighting the critical role of local flow dynamics in determining long-term outcomes. By enabling non-invasive assessment and improved surgical planning, this validated CFD workflow, combined with 4D Flow particle tracking, offers immediate clinical application. With CFD-based surgical planning gaining traction, this approach establishes a new standard for personalized management for patients undergoing treatment for congenital heart diseases.SummaryThis study presents a validated workflow integrating 4D Flow CMR and patient-specific CFD to accurately quantify hepatic flow distribution and enhance surgical planning, addressing key challenges in the treatment and long-term management of Fontan patients with single-ventricle disease.
Magnetically induced magnetosome chain (MAGiC): A biogenic magnetic-particle-imaging tracer with high performance and navigability
Authors: Li Lei , Zhao Chan , Zhao Zhiyuan , Han Dou , Liao Yidong , Liu Yanjun , Liu Sijia , Jia Xiaohua , Tian Jiesheng , Liu Qing , Feng Xin , Tian Jie 
Published: December 2024

AbstractMagnetic particle imaging (MPI) enables real-time, sensitive and quantitative visualization of magnetic tracers’ spatial distribution, augmenting the capability of in vivo imaging technologies. Previous tracer studies in MPI have primarily focused on superparamagnetic nanoparticles; however, their non-ideal sigmoidal magnetization response limits the spatial resolution. Here we demonstrate the utilization of magnetically induced magnetosome chain (MAGiC) as a novel superferromagnetic MPI tracer, exhibiting a 25-fold improvement in resolution and a 91-fold enhancement in signal intensity compared to the commercial tracer VivoTrax+™. The spatial resolution of MPI was pushed to an unprecedented 80 μm under a 4 T/m gradient field. Additionally, MAGiC can be precisely controlled using magnetic fields, enabling it to function as a MPI trackable microrobot. We provided a theoretical model elucidating MAGiC’s unique properties, and validated its imaging and actuation performance through phantom studies and in vivo experiments. As a high-performance MPI tracer and magnetic microrobot with exceptional capabilities, MAGiC holds tremendous potential for diverse applications including cell tracking, targeted drug delivery as well as therapeutic interventions.
Going beyond cell clustering and feature aggregation: Is there single cell level information in single-cell ATAC-seq data?
Authors: Kwok Aaron Wing Cheung , Shim Heejung , McCarthy Davis J 
Published: December 2024

1AbstractSingle-cell Assay for Transposase Accessible Chromatin with sequencing (scATAC-seq) has become a widely used method for investigating chromatin accessibility at single-cell resolution. However, the resulting data is highly sparse with most data entries being zeros. As such, currently available computational methods for scATAC-seq feature a range of transformation procedures to extract meaningful information from the sparse data. Most notably, these transformations can be categorized into: 1) feature aggregation with known biological associations, 2) pseudo-bulking cells of similar biology, and 3) binarisation of count data. These strategies beg the question of whether or not scATAC-seq data actually has usable single-cell and single-region information as intended from the assay. If we can go beyond aggregated features and pooled cells, it opens up the possibility of more complex statistical tasks that require that degree of granularity. To reach the finest possible resolution of single-cell, single-region information there are inevitably many computational challenges to overcome. Here, we review the major data analysis challenges lying between raw data readout and biological discovery, and discuss the limitations of current data analysis approaches. Lastly, we conclude that chromatin accessibility profiling at true single-cell resolution is not yet achieved with current technology, but that it may be achieved with promising developments in optimising the efficiency of scATAC-seq assays.
New insight into erythema reduction induced by quercetin metal ions chelates
Authors: Bolaño Alvarez Alain , Arvesen Kristian B. , Hjuler Kasper F. , Bjerring Peter , Petersen Steffen B. 
Published: December 2024

AbstractIn dermatology, chelates play a significant role in skin care and treatment of skin diseases. Chelates involve coordination bonding between metal ions and organic molecules such as flavonoids. Quercetin (Q) is an extensively studied natural flavonoid with proven safety and anti-inflammatory properties. This study shows the role of two biocompatible metal ions, Iron (Fe2+) and Copper (Cu2+) in coordination bonding withQin 2-Propanol 50% and 80% at 1:1 stoichiometry. Our results show that chelation involves the hydroxyl groups and occurs by coordination ofCu2+toQfor the Ring A-B (Benzoyl group) resulting in a fluorescence emission peak at 530nm from the Ring B-C (Cinnamoyl group). This chelatesQ+Cu2+reduces mechanically induced erythema in the skin (Tanned type). A similar effect was observed in the chelateQ+Fe2+where the coordination ofFe2+toQoccurs for the cinnamoyl group resulting in an emission peak at 425nm from Benzoyl group ofQ. The statistical analysis shows significant differences in the effects ofQ+Cu2+(p-value = 0.00001),Q+Fe2+(p-value = 0.0003) respect toQas well as between them (p-value =0.0029). Our results suggest that the interaction betweenQand metal ions plays a central role in the inflammatory pathway. We conclude that the anti-inflammatory properties ofQwere enhanced by bothQ+Fe2+andQ+Cu2+chelates, highlighting the effect ofQ+Fe2+where the hydroxyl groups available in the cinnamoyl group of theQmolecule are the main intermediates to interact withFe2+, which is a requirement to trigger the anti-inflammatory molecular events ofQmolecules.
Differing genetics of saline and cocaine self-administration in the hybrid mouse diversity panel
Authors: Khan Arshad H. , Bagley Jared R. , LaPierre Nathan , Gonzalez-Figueroa Carlos , Spencer Tadeo C. , Choudhury Mudra , Xiao Xinshu , Eskin Eleazar , Jentsch James D. , Smith Desmond J. 
Published: December 2024

AbstractTo identify genes involved in regulating the behavioral and brain transcriptomic response to the potentially addictive drug cocaine, we performed genome-wide association studies (GWASs) for intravenous self-administration of cocaine or saline (as a control) over 10 days using a panel of inbred and recombinant inbred mice. A linear mixed model increased statistical power for these longitudinal data and identified 145 loci for responding when saline only was delivered, compared to 17 for the corresponding cocaine GWAS. Only one locus overlapped. Transcriptome-wide association studies (TWASs) using RNA-Seq data from the medial frontal cortex and nucleus accumbens identified5031434O11RikandZfp60as significant for saline self-administration. Two other genes,Myh4andNpc1, were nominated based on proximity to loci for multiple endpoints or acislocus regulating expression. All four genes have previously been implicated in locomotor activity. Our results indicate distinct genetic bases for saline and cocaine self-administration, and suggest some common genes for saline self-administration and locomotor activity.
Neurotransmitter loaded DNA nanocages as potential therapeutics for α-synuclein based neuropathies in cells andin vivo
Authors: Vaswani Payal , Kansara Krupa , Kumar Ashutosh , Bhatia Dhiraj 
Published: December 2024

AbstractParkinson’s disease is one of the neuropathies characterized by accumulation of α-synuclein protein, leading to motor dysfunction. Levodopa is the gold standard treatment, however, in long term usage, it leads to levodopa induced dyskinesia (LID). New therapeutic options are need of the hour to treat the α-synuclein based neuropathies. The role of imbalance of neurotransmitters other than dopamine has been underestimated in α-synuclein based neuropathies. Here, we explore the role of serotonin, epinephrine and norepinephrine as a therapeutic moiety. For the efficientin vivodelivery, we use DNA nanotechnology-based DNA tetrahedra that has shown the potential to cross the biological barriers. In this study, we explore the use of DNA nanodevices, particularly DNA tetrahedron functionalized with neurotransmitters, as a novel therapeutic approach for MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) induced Parkinson’s disease in PC12 cellular system. We first establish the effect of these nanodevices on clearance of α-synuclein protein in cells. We follow the study by understanding the various cellular processes like ROS, iron accumulation and lipid peroxidation. We also explore the effect of the neurotransmitter loaded nanodevices inin vivozebrafish model. We show that neurotransmitter loaded DNA nanocages can potentially clear the MPTP induced α-synuclein aggregates in cells andin vivo. The findings of these work open up new avenues for use of DNA nanotechnology by functionalizing it with neurotransmitters for future therapeutics in treatment of neurodegenerative diseases such as Parkinson’s disease.TOCTD:NT can clear α-synuclein by targeting the ferroptosis pathway.