bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA‐29b‐3p reduces intestinal ischaemia/reperfusion injury via targeting of TNF receptor‐associated factor 3
Authors: Dai Yan , Mao Zhang , Han Xu , Xu Youwei , Xu Lina , Yin Lianhong , Qi Yan , Peng Jinyong 
Published: June 2019

Background and PurposeThe microRNA miR‐29b‐3p shows important roles in regulating apoptosis and inflammation. However, its effects on intestinal ischaemia/reperfusion (II/R) injury have not been reported. Here we have investigated the functions of miR‐29b‐3p on II/R injury on order to find drug targets to treat the injury.Experimental ApproachTwo models ‐ in vitro hypoxia/reoxygenation (H/R) of IEC‐6 cells; in vivo, II/R injury in C57BL/6 mice were used. Western blotting and dual‐luciferase reporter assays were used and mimic and siRNA transfection tests were applied to assess the effects of miR‐29b‐3p on II/R injury via targeting TNF receptor‐associated factor 3 (TRAF3).Key ResultsThe H/R procedure decreased cell viability and promoted inflammation and apoptosis in IEC‐6 cells, and the II/R procedure also promoted intestinal inflammation and apoptosis in mice. Expression levels of miR‐29b‐3p were decreased in H/R‐induced cells and II/R‐induced intestinal tissues of mice compared with control group or sham group, which directly targeted TRAF3. Decreased miR‐29b‐3p level markedly increased TRAF3 expression via activating TGF‐α‐activated kinase 1 phosphorylation, increasing NF‐κB (p65) levels to promote inflammation, up‐regulating Bcl2‐associated X expression, and down‐regulating Bcl‐2 expression to trigger apoptosis. In addition, the miR‐29b‐3p mimetic and TRAF3 siRNA in IEC‐6 cells markedly suppressed apoptosis and inflammation to alleviate II/R injury via inhibiting TRAF3 signallimg.Conclusions and ImplicationsThe miR‐29b‐3p played a critical role in II/R injury, via targeting TRAF3, which should be considered as a significant drug target to treat the disease.
Safety and efficacy of endoscopic ultrasound‐guided fine needle aspiration for pancreatic masses: A prospective multicenter study
Authors: Yoshinaga Shigetaka , Itoi Takao , Yamao Kenji , Yasuda Ichiro , Irisawa Atsushi , Imaoka Hiroshi , Tsuchiya Takayoshi , Doi Shinpei , Yamabe Akane , Murakami Yoshitaka , Ishikawa Hideki , Saito Yutaka 
Published: June 2019

ObjectivesEndoscopic ultrasound‐guided fine needle aspiration (EUS‐FNA) for solid pancreatic lesions has high diagnostic yield. However, few prospective multicenter studies have been performed. We performed a prospective cohort study to evaluate the efficacy and safety of EUS‐FNA for diagnosis of solid pancreatic lesions.MethodsThis prospective cohort study involved five hospitals in Japan. The primary outcome was sensitivity of EUS‐FNA for diagnosing malignant lesions. We also evaluated parameters of diagnostic sufficiency and the safety of EUS‐FNA.ResultsIn total, 246 patients were enrolled. The absolute values of the parameters evaluated showed no significant differences; however, the percentage changes in the white blood cell counts and C‐reactive protein levels after examination were significantly higher, and the percentage change in hemoglobin concentrations was significantly lower. The minor and major complication rates at the time of puncture, 24 h, 7 days and 28 days were 4.1%, 2.8%, 1.6%, and 0.0%, respectively. The true complication rate was 1.2%. The diagnostic sensitivity, specificity, accuracy, positive predictive value, and negative predictive value were 97.2%, 88.0%, 96.2%, 100%, and 81.4%, respectively.ConclusionsEUS‐FNA for solid pancreatic lesions has high diagnostic yield and is safe, consistent with previously studies.
Tissue Doppler imaging of the atrial lateral wall: Correlation with atrial strain rate and parameters of diastolic function
Authors: Argento Laura V. , Travetto Carolina M. , de las M. Colicigno Maria , Gentile Silvia , Salvati Ana , Lax Jorge , Cianciulli Tomás 
Published: June 2019

AbstractObjectiveTo evaluate the velocity profile of the lateral wall of the left atrium (LA) using pulsed‐wave color tissue Doppler imaging (TDI) on line and its correlation with two‐dimensional strain rate imaging (SRI) by speckle tracking, of equal segments and phases, and other parameters of diastolic function in a population of individuals without known cardiovascular disease.Materials and MethodsA total of 91 adults aged 18–74 years, without cardiovascular history, hypertension, diabetes, dyslipidemia or obesity, were evaluated. A Doppler echocardiogram was performed. The lateral wall of the LA was divided subjectively into three portions, the atrial roof was excluded; the peak velocities of the basal and midportions, and their average, were measured with pulsed‐wave TDI on line and SRI. Using both techniques, a first positive wave (S'la and SRS‐reservoir phase) and two negative waves (E'la and SRE—conduit phase—and A'la and SRA—atrial systole—respectively) were obtained. E'la/A'la ratio and SRE/SRA ratio were analyzed.ResultsThe median age was 42 years, and 54.9% of the subjects were female. S'la presented moderate correlation with S' lateral mitral annulus. Both E'la and the E'la/A'la relationships showed moderate to good correlation with various diastolic dysfunction parameters evaluated and SRI. A'la did not present a statistically significant correlation with any of the variables evaluated.ConclusionEvaluation of the lateral wall of the LA by pulsed‐wave color TDI on line is feasible in the vast majority of patients. In the conduit phase, E'la and the E'la/A'la relationship showed correlation with the parameters that evaluate diastolic function and SRI.
Apolipoprotein E genetic variants interact with Mediterranean diet to modulate postprandial hypertriglyceridemia in coronary heart disease patients: CORDIOPREV study
Authors: Gomez‐Delgado Francisco , Alcala‐Diaz Juan Francisco , Leon‐Acuña Ana , Lopez‐Moreno Javier , Delgado‐Lista Javier , Gomez‐Marin Beatriz , Roncero‐Ramos Irene , Yubero‐Serrano Elena M. , Rangel‐Zuñiga Oriol Alberto , Vals‐Delgado Cristina , Luque Raul M. , Ordovas Jose M. , Lopez‐Miranda Jose , Perez‐Martinez Pablo 
Published: June 2019

AbstractBackgroundWe try to explore whether long‐term consumption of two healthy dietary patterns (low‐fat [LF] diet or Mediterranean diet [MedDiet]) interacts with the apolipoprotein E (APOE) single‐nucleotide polymorphisms (SNPs: rs439401, rs440446 and rs7412) modulating postprandial hypertriglyceridemia (ppHTG) in coronary heart disease (CHD) patients.Methods and resultsWe selected patients from the CORDIOPREV study with genotyping and who underwent an oral fat load test (FLT) at baseline and after 3 years follow‐up (n = 506). After 3 years of follow‐up, we found a gene‐diet interaction between the APOE rs439401 SNP and MedDiet. Specifically, T‐allele carriers in the MedDiet group showed a more significant decrease in postprandial triglycerides (TG: P = 0.03) and large triacylglycerol‐rich lipoproteins (TRLs) TG (large TRLs TG; P = 0.01) compared with CC subjects. Consistently, the area under the curve of TG (AUC‐TG; P‐interaction = 0.03) and AUC‐large TRLs TG (P‐interaction = 0.02) were significantly lower in T‐allele carriers compared with CC subjects.ConclusionsThe long‐term consumption of a MedDiet modulates ppHTG through APOE genetic variants in CHD patients. This gene‐diet interaction may contribute to a more precise dietary advice in CHD patients.
Anthropogenic nitrogen enrichment enhances soil carbon accumulation by impacting saprotrophs rather than ectomycorrhizal fungal activity
Authors: Maaroufi Nadia I. , Nordin Annika , Palmqvist Kristin , Hasselquist Niles J. , Forsmark Benjamin , Rosenstock Nicholas P. , Wallander Håkan , Gundale Michael J. 
Published: June 2019

AbstractThere is evidence that anthropogenic nitrogen (N) deposition enhances carbon (C) sequestration in boreal forest soils. However, it is unclear how free‐living saprotrophs (bacteria and fungi, SAP) and ectomycorrhizal (EM) fungi responses to N addition impact soil C dynamics. Our aim was to investigate how SAP and EM communities are impacted by N enrichment and to estimate whether these changes influence decay of litter and humus. We conducted a long‐term experiment in northern Sweden, maintained since 2004, consisting of ambient, low N additions (0, 3, 6, and 12 kg N ha−1 year−1) simulating current N deposition rates in the boreal region, as well as a high N addition (50 kg N ha−1 year−1). Our data showed that long‐term N enrichment impeded mass loss of litter, but not of humus, and only in response to the highest N addition treatment. Furthermore, our data showed that EM fungi reduced the mass of N and P in both substrates during the incubation period compared to when only SAP organisms were present. Low N additions had no effect on microbial community structure, while the high N addition decreased fungal and bacterial biomasses and altered EM fungi and SAP community composition. Actinomycetes were the only bacterial SAP to show increased biomass in response to the highest N addition. These results provide a mechanistic understanding of how anthropogenic N enrichment can influence soil C accumulation rates and suggest that current N deposition rates in the boreal region (≤12 kg N ha−1 year−1) are likely to have a minor impact on the soil microbial community and the decomposition of humus and litter.
Breast implant‐associated anaplastic large cell lymphoma (BIA‐ALCL): an overview of presentation and pathogenesis and guidelines for pathological diagnosis and management
Authors: Jones J Louise , Hanby Andrew M , Wells Clive , Calaminici Marie , Johnson Laura , Turton Philip , Deb Rahul , Provenzano Elena , Shaaban Abeer , Ellis Ian O , Pinder Sarah E ,   on behalf of the National Co‐ordinating Committee of Breast Pathology
Published: June 2019

AimsBreast implant‐associated anaplastic large cell lymphoma (BIA‐ALCL) is an uncommon complication associated largely with textured implants. It is important that the symptoms associated with BIA‐ALCL are recognised and that robust pathways are in place to establish the diagnosis. The aim of this paper is to review what is known of the incidence of the disease, current thoughts on pathogenesis, patterns of presentation and pathological features to provide standard guidelines for its diagnosis.Methods and resultsSystematic review of the literature via PubMed covering cases series, modes of presentation, cytological, histological and immunohistochemical features and disease outcome. Since 1997, 518 cases throughout 25 countries have been registered on the American Society of Plastic Surgeons PROFILE registry, with an estimated risk for women with an implant of one to three per million per year. It most frequently presents as a late‐onset accumulation of seroma fluid, sometimes as a mass lesion. The neoplastic cells are highly atypical, consistently strongly positive for CD30, with 43–90% also positive for EMA, and all are ALK‐negative. Behaviour is best predicted using a staging system for solid tumours.ConclusionBIA‐ALCL is a rare but important complication of breast implants. While characterised by CD30‐positive neoplastic cells this must be interpreted with care, and we provide pathological guidelines for the robust diagnosis of this lesion as well as the most appropriate staging system and management strategies. Finally, in order to generate more accurate data on incidence, we recommend mechanisms for the routine central reporting of all cases.
18β‐Glycyrrhetinic acid: its core biological properties and dermatological applications
Authors: Kowalska A. , Kalinowska‐Lis U. 
Published: June 2019

AbstractRecently, attention has been focused on identifying natural herbal compounds with high biological activity, especially antioxidative, anti‐inflammatory and antimicrobial properties, for preventing and controlling various skin conditions, including inflammation‐related diseases such as atopic dermatitis and UV‐induced skin photoaging. One key active plant ingredient is 18β‐glycyrrhetinic acid (GA), the main metabolite of glycyrrhizin (GL), obtained from licorice root. The review examines the valuable biological properties of GA, particularly those playing key roles in the treatment of various dermatological disorders in humans. The review highlights the key anti‐inflammatory, antioxidant and antimicrobial properties of GA and its toxicity towards normal cells lines. It also examines the physicochemical properties of GA and presents methods of increasing its penetration through the stratum corneum and bioaccumulation with the use of modern delivery systems such as liposomes and nanoemulsions.
Climatic conditions and human activities shape diploid–tetraploid coexistence at different spatial scales in the common weedTripleurospermum inodorum(Asteraceae)
Authors: Čertner Martin , Kúr Pavel , Kolář Filip , Suda Jan 
Published: June 2019

AbstractAimWhile environmental conditions are reported as important determinants of cytotype distribution patterns in some mixed‐ploidy plant species, in others they seem to play no role. One reason for such inconsistency might be how and at what spatial scale the ecology of different cytotypes is compared. To address this issue, we adopted several complementary approaches to assess ecological requirements of cytotypes in a single species across wide‐range of spatial scales.LocationEurope.TaxonTripleurospermum inodorum(Asteraceae).MethodsWorldClim extracted data were used for comparing climatic niches ofT. inodorumdiploids and tetraploids at continental and subcontinental scales and for predicting climatic suitability of habitats in Europe. Within the contact zone of cytotypes, at the regional scale, ecological preferences of cytotypes were assessed independently in three regions. Habitat types and ecological indicators inferred from species composition of vegetation in both uniform‐ploidy and mixed‐ploidy populations were used for ecological niche reconstructions.ResultsThe two cytotypes differed in their climatic niches. However, due to considerable niche overlap, diploids and tetraploids were predicted to co‐occur in an extensive contact zone in Europe. Within the contact zone, cytotypes had identical ecological preferences but varied in their frequency at particular habitat types. The ecological niches of mixed‐ploidy and uniform‐ploidy populations were undistinguishable.Main conclusionsCytotype distribution patterns may be shaped by multiple environmental forces, each operating at a different spatial scale. InT. inodorum, climate is an important driver of cytotype distribution patterns at continental scale, however, at finer scales within the contact zone, the founder effect and non‐random migration patterns caused by human activities seem to be more important than environmental heterogeneity. The use of independent replicates and/or across‐scale comparisons can significantly improve the performance of ecological niche assessments.
Providing Behavioral Health Services in Nursing Homes Is Difficult: Findings From a National Survey
Authors: Orth Jessica , Li Yue , Simning Adam , Temkin‐Greener Helena 
Published: June 2019

OBJECTIVESBehavioral health (BH) disorders affect 65% to 90% of nursing home (NH) residents. Access to BH services in NHs has been generally considered inadequate, but the empirical evidence is sparse. We examined the availability of BH services and identified facility‐level factors associated with the difficulty of providing BH services in NHs.DESIGNA national random sample of 3996 NHs was identified. Two structured surveys with questions about BH service availability, quality, satisfaction, staffing, staff education, turnover, and service barriers were mailed to administrators and directors of nursing in each NH between July and December 2017.SETTING/PARTICIPANTSCompleted surveys were obtained from 1079 NHs (27% response rate). Descriptive statistics and multivariable logistic regressions were employed.MEASUREMENTSFour outcome measures were based on five‐point Likert scales: (1) adequacy of BH staff education; (2) ability to meet resident BH service needs; (3) adequacy of coordination/collaboration between NH/community providers; and (4) availability of necessary facility infrastructure.RESULTSBH service needs were unmet in one third of NHs; almost half lacked appropriate staff BH education. Over 30% reported having inadequate coordination of care between NH and community providers, and 26.2% had inadequate infrastructure for residents' referrals/transport. Staff BH education was less problematic in NHs with Alzheimer disease units (odds ratio [OR] = 0.6; P < .05), lower registered nurse (RN) turnover (OR = 0.7; P < .05), and more psychiatrically trained RNs (OR = 0.5; P < .001) and social workers (OR = 0.6; P < .05). Lower RN turnover (OR = 0.7; P < .05) and more psychiatrically trained RNs (OR = 0.6; P < .05) were associated with fewer NHs reporting being unable to meet BH service needs. Having more psychiatrically trained RNs (OR = 0.6; P < .05) was associated with fewer NHs reporting inadequate coordination with community providers.CONCLUSIONInadequate BH education and psychiatric training among NH staff were associated with subpar provision of BH services in this care setting. New initiatives that increase access to BH providers and services and improve staff education are urgently needed in NHs. J Am Geriatr Soc 67:1713–1717, 2019
Nurses' attitudes towards support for and communication about sexual health—A qualitative study from the perspectives of oncological nurses
Authors: Annerstedt Catharina Frid , Glasdam Stinne 
Published: June 2019

AbstractAim and objectivesTo explore nurses' articulations of support and communication regarding sexual health with patients.BackgroundSexual health is adversely effected by cancer and various oncological treatments. Patients' often have the experience that healthcare professionals do not talk about sexual health.MethodSemi‐structured interviews were conducted with seven nurses in Southern Sweden. Content analyses were made, inspired by Foucault's concepts of power, discipline and normalisation. SRQR checklist was used.ResultsPatients' sexual health had low priority in the oncological clinic from the perspective of nurses. The medical logic directed nurses' articulations about sexual health towards a physical view, understood as sex, and sexual problems, which could be treated pharmacologically. Further, nurses articulated a sexual norm that sex belongs to young people and younger persons in permanent, monogamous and heterosexual relationships. This norm‐governed nurses' inclusion and exclusion of patients in communication about sexual health. According to nurses, most patients did not mention sex, but some patients challenged the clinic's norms. Assessing sexual health problems, nurses often engaged other professions and thereby became gatekeepers for patients' options for getting help.ConclusionsFrom the perspective of nurses, nurses' support and communication regarding sexual health with patients with cancer diagnosis were relatively absent and had a low priority in an oncological clinic. Overall, the nurses had the power to set the agenda about patients' sexual health in the oncological clinic. The clinical gaze became a disciplinary technique that tacitly defined by whom, in what way and how sexual health could be articulated in an oncological clinic.Relevance to clinical practiceBy illuminating nurses' preconceptions, thoughts and actions in relation to cancer patients' sexual health, the results invite practitioners to reflect upon and discuss the challenges, opportunities and limitations in providing inclusive and supportive sexual health care to cancer patients.
Long non‐coding RNA myocardial infarction associated transcript promotes epithelial‐mesenchymal transition and is an independent risk factor for poor prognosis of tongue squamous cell carcinoma
Authors: Zhong Waisheng , Xu Zi , Wen Senli , Xie Tao , Wang Fang , Wang Qiwei , Chen Jie 
Published: June 2019

AbstractBackgroundLong non‐coding RNA myocardial infarction associated transcript (MIAT) has been suspected to be associated with poor prognosis in several malignancies. This study aims to investigate the association between MIAT expression levels and prognosis of tongue squamous cell carcinoma (TSCC).Materials and MethodsExpressions of MIAT in TSCC specimens, corresponding adjacent non‐neoplastic tongue tissues, and serums collected from 116 TSCC patients were detected by Quantitative Real‐Time PCR. Then, these patients were followed up for 12 months after discharge. MIAT was suppressed and upregulated in TSCC cells, and then cell invasion and epithelial‐mesenchymal transition (EMT) markers were analyzed.ResultsMyocardial infarction associated transcript expression level in TSCC specimen was upregulated compared to adjacent non‐neoplastic tongue tissue and had a significant positive association with its level in serum. MIAT levels in both TSCC specimen and serum were correlated with cervical lymph node metastasis and histological grading of TSCC patients. Results of survival analysis showed that high expression levels of MIAT in both TSCC tissues and serums indicated higher mortality and shorter survival time of TSCC patients. Receiver operating characteristic curve analysis showed that there was no significant difference between MIAT in TSCC specimen and serum to differentiate death and non‐death of TSCC patients. In vitro study showed that MIAT could induce invasion of TSCC cells by regulating expressions of EMT markers through activation of Wnt/β‐catenin signaling pathway.ConclusionUpregulated MIAT promoted EMT of TSCC cells through activation of Wnt/β‐catenin signaling pathway and indicated a poor prognosis of TSCC, which may provide us a non‐invasive approach to evaluate the prognosis of TSCC.
Effect of culturing parameters on the vegetative growth of Haematococcus alpinus (strain lcr‐cc‐261f) and modeling of its growth kinetics
Authors: Mazumdar Nilanjana , Novis Phil M. , Visnovsky Gabriel , Gostomski Peter 
Published: June 2019

The present study investigated the effect of different culture conditions on the vegetative growth of a new species, Haematococcus alpinus (strain LCR‐CC‐261f) using airlift photobioreactors. The influence of culture medium, aeration rates, CO2 concentration in air‐gas mixture, temperature, light intensities, and wavelengths were investigated to achieve sustainable high cell density cultures. Growth parameters were determined by fitting the data to a form of the logistic equation that included a lag phase. The shear‐sensitive vegetative cells favored lower aeration rates in the photobioreactors. MLA medium increased to 40 mM nitrate produced high density cultures. Temperatures between 12°C and 18°C, 3% (v/v) CO2 concentration and a narrow photon flux density ranging between 37 and 48 μmol photons · m−2 · s−1 were best suited for growth. The wavelength of the light source also impacted growth and a high cell density of 9.6 × 105 cells · mL−1 was achieved using a mixture of red and blue compared to warm white, red, or blue LEDs.