bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microwave vs radiofrequency ablation for hepatocellular carcinoma within the Milan criteria: a propensity score analysis
Authors: Liu Wenwu , Zheng Yun , He Wei , Zou Ruhai , Qiu Jiliang , Shen Jingxian , Yang Zhiwen , Zhang Yuanping , Wang Chenwei , Wang Yongjin , Zuo Dinglan , Li Binkui , Yuan Yunfei 
Published: July 2018

SummaryBackgroundWhether microwave ablation (MWA) challenges the standard role of radiofrequency ablation (RFA) in treating early‐stage hepatocellular carcinoma (HCC) remains unclear.AimTo compare the efficacy of MWA vs RFA for treating primary HCC within the Milan criteria.MethodsFrom January 2002 to January 2017, the oncological outcomes after MWA (126 patients) and RFA (436 patients) were analysed by propensity score matching.ResultsBefore propensity score matching, for overall patients, MWA resulted in similar 5‐year overall survival to RFA (80.1% vs 75.8%, P = 0.190) but better 5‐year recurrence‐free survival (28.1% vs 19.6%, P = 0.036). For solitary HCC ≤ 3 cm, MWA resulted in comparable 5‐year overall survival (81.8% vs 77.1%, P = 0.170) to RFA but better 5‐year recurrence‐free survival (34.6% vs 24.0%, P = 0.042). After propensity score matching, MWA resulted in better 5‐year overall survival (79.3% vs 68.4%, P = 0.021) and 5‐year recurrence‐free survival (27.9% vs 6.4%, P < 0.001) than RFA for HCC. For solitary HCC ≤3 cm, MWA resulted in comparable 5‐year overall survival (81.2% vs 66.3%, P = 0.062) and 5‐year recurrence‐free survival (37.7% vs 17.4%, P = 0.088) to RFA. In Cox analysis, RFA modality, tumours located in risk areas and low serum albumin levels were unfavourable prognostic factors for overall survival. RFA modality, multiple tumours, tumour size and low serum albumin levels were unfavourable prognostic factors for recurrence‐free survival (all P < 0.05).ConclusionsRFA is inferior to MWA for treating HCC within the Milan criteria, but has comparable efficacy to MWA for solitary HCC ≤ 3 cm.
Microanatomy of the testes and testicular ducts of the butterfly lizard, Leiolepis ocellata Peters, 1971 (Reptilia: Squamata: Agamidae) during the active reproductive period
Authors: Pewhom Akkanee , Srakaew Nopparat 
Published: July 2018

AbstractThe microanatomy of the testes and testicular ducts (rete testis, ductuli efferentes, ductus epididymis and ductus deferens) of Leiolepis ocellata (Agamidae) was investigated using light microscopy including histochemistry. Each testis contains seminiferous tubules and interstitial tissues. The former house spermatogenic cells (spermatogonia A & B, preleptotene, primary and secondary spermatocytes, spermatids (steps 1–8) and spermatozoa) and Sertoli cells, while the latter comprise peritubular and intersitial tissues. The rete testis is an anastomosing duct, having intratesticular and extratesticular portions. The proximal region of ductuli efferentes has wider outer ductal and luminal diameters than those of the distal region. The convoluted ductus epididymis is subdivided into four regions (initial segment, caput, corpus and cauda), based on the ductal diameter, epithelium characteristics and cell components. The ductus deferens has the greatest diameter and is divided into the ductal and ampulla ductus deferens. The ductal portion is subdivided into the proximal and distal regions, based on the epithelium types and ductal diameters. The ampulla ductus deferens is a fibromuscular tube, having numerous mucosal folds projecting into the lumen. Spermiophagy is detectable in the ductus epididymis and ductus deferens. The present results contribute to improved fundamental knowledge on the microanatomy of the reptilian reproductive system.
Long non‐coding RNA FAL1 functions as a ceRNA to antagonize the effect of miR‐637 on the down‐regulation of AKT1 in Hirschsprung's disease
Authors: Li Yang , Zhou Lingling , Lu Changgui , Shen Qiyang , Su Yang , Zhi Zhengke , Wu Feng , Zhang Hua , Wen Zechao , Chen Guanglin , Li Hongxing , Xia Yankai , Tang Weibing 
Published: July 2018

AbstractObjectivesEmerged evidence demonstrates that long non‐coding RNAs (lncRNAs) may play quintessential regulatory roles in the cellular processes, tumourigenesis and the development of disease. Though focally amplified lncRNA on chromosome 1 (FAL1) has been identified to have crucial functions in many diseases, its biological mechanism in the development of Hirschsprung's disease (HSCR) still remains unknown.Materials and methodsThe expression levels of FAL1 in HSCR aganglionic tissues and matched normal specimens were detected by quantitative real‐time PCR (qRT‐PCR). Cell proliferation and migration were detected by Cell Counting Kit‐8 (CCK‐8) assay, Ethynyl‐deoxyuridine (EdU) assay and transwell assay relatively. Cell cycle and apoptosis were assessed using flow cytometer analysis. Moreover, the novel targets of FAL1 were confirmed with the help of bioinformatics analysis and dual‐luciferase reporter assay. Western blot assay as well as RNA immunoprecipitation (RIP) assay was conducted to investigate the potential mechanism.ResultsFAL1 expression was markedly down‐regulated in HSCR aganglionic tissues and decreased FAL1 expression was associated with the diagnosis of HSCR. Cell functional analyses indicated that FAL1 overexpressing notably promoted cell proliferation and migration, while down‐regulation of FAL1 suppressed cell proliferation and migration. Additionally, Flow cytometry assay demonstrated that knockdown of FAL1 induced markedly cell cycle stalled in the G0/G1 phase. Furthermore, FAL1 could positively regulate AKT1 expression by competitively binding to miR‐637.ConclusionsThese results illuminated that FAL1 may work as a ceRNA to modulate AKT1 expression via competitively binding to miR‐637 in HSCR, suggesting that it may be clinically valuable as a biomarker of HSCR.
Prediction of coronary heart disease or heart failure using high‐sensitivity cardiac troponin T: A pilot study
Authors: Árnadóttir Ásthildur , Vestergaard Kirstine Roll , Sölétormos György , Steffensen Rolf , Goetze Jens P. , Iversen Kasper 
Published: July 2018

AbstractBackgroundHigh‐sensitivity cardiac troponin T (hs‐cTnT) is a good prognostic marker for mortality. However, it is uncertain if hs‐cTnT can be used to detect sub‐clinical cardiac disease.MethodPilot study in patients without known heart disease and elevated hs‐cTnT measured at presentation to the emergency department. Hs‐cTnT was measure with Roche Diagnostics. Echocardiography was used to assess structural heart disease and the participants underwent computed tomography angiography for assessment of coronary artery disease and agatston score.ResultsTen patients were included in the final cohort. Median age was 68 years IQR (57‐78) and 80% were female (n = 8). Six patients had a history of chronic obstructive lung disease and five patients had history of hypertension. The median level of hs‐cTnT was 26 ng/L and values ranged from 19 ng/L to 495 ng/L. The median calcium score was 12. Three patients had signs of coronary artery disease. All patients had normal left ventricular ejection fraction with a median LVEF at 54.5%. Two patients were noted to have increased left ventricular mass index (LVMI).ConclusionThe majority of patients with hs‐cTnT above the 99th percentile did not have structural heart disease or ischaemic coronary disease. However, 30% of the patient did have signs of coronary disease and might benefit from preventive medical treatment. Measuring hs‐cTnT in the absence of acute illness might be a better approach for evaluation for sub‐clinical cardiac disease.
Modelling the removal and reversible immobilization of murine noroviruses in a Phaeozem under various contamination and rinsing conditions
Authors: Tesson V. , de Rougemont A. , Capowiez L. , Renault P. 
Published: July 2018

Enteric viruses entering the soil with contaminated irrigation water can reach groundwater or be internalized in plants through their roots without being inactivated. Their fate in the soil depends on the virus, the soil and the soil solution. In order to write a mathematical model suitable for a Calcaric Phaeozem, we investigated the removal of murine norovirus and reversible immobilization in aggregate columns according to a saturation procedure, conditions between contamination and rinsing time, temperature and soil solution. Viruses were quantified before and after 0.45‐μm filtration with an RT‐qPCR (real‐time polymerase chain reaction). Experimental results supported a model that combined free and colloidal transport of viruses in mobile water, exchange of free viruses between mobile and immobile water, virus removal and reversible virus adsorptions on suspended colloids, the outer aggregate surface and the inner aggregate particles. For an artificial soil solution at 20°C, the fate of viruses in contaminations lasting 1 to 7 days followed by 7 hours of rinsing was described by combining 0.38 log10 daily removal and weak reversible immobilization using a Freundlich adsorption isotherm (kF = 1120, nF = 1.53), which explained why free viruses prevailed in mobile water. Partial drying without aggregate desaturation did not affect virus recovery. Magnesium cation enrichment induced geochemical changes that faded over time, resulting in up to ten times more viruses adsorbed on suspended colloids than free and enhanced adsorption on outer aggregate surfaces. Likewise, groundwater rich in Mg2+ slowed remobilization. The fate of murine norovirus within a Calcaric Phaeozem can be described by a model that takes into account geochemical fluctuations.Highlights
How far does soil reduce the risk of noroviruses from irrigation reaching groundwater or roots?
Virus removal over a 5‐day average residence time in Phaeozem is about two log10.
Reversible virus immobilization is weak; Mg intake favours it, together with colloidal transport.
Virus removal and reversible adsorption can be modelled for better assessment of contamination risk.
The neuropsychiatric phenotype in CACNA1A mutations: a retrospective single center study and review of the literature
Authors: Indelicato E. , Nachbauer W. , Karner E. , Eigentler A. , Wagner M. , Unterberger I. , Poewe W. , Delazer M. , Boesch S. 
Published: July 2018

Background and purposeCACNA1A encodes the α1 subunit of the neuronal calcium channel P/Q. CACNA1A mutations underlie three allelic disorders: familial hemiplegic migraine type 1 (FHM1), episodic ataxia type 2 (EA2) and spinocerebellar ataxia type 6 (SCA6). A clear‐cut genotype–phenotype correlation is often lacking since clinical manifestations may overlap. Several case reports have described cognitive and behavioral features in CACNA1A disorders, but studies in larger case series are lacking.MethodsGenetically confirmed CACNA1A cases were retrieved from the database of the ataxia outpatient clinic of the Department of Neurology at Innsbruck Medical University. Clinical charts and neuropsychological test results were retrospectively analyzed. In addition, a review of the literature including only genetically confirmed cases was performed.ResultsForty‐four CACNA1A cases were identified in our database. Delayed psychomotor milestones and poor school performance were described in seven (four FHM1, three EA2) and eight (three FHM1, five EA2) patients, respectively. Psychiatric comorbidities were diagnosed in eight patients (two FHM1, six EA2). Neuropsychological testing was available for 23 patients (11 FHM1, 10 EA2, two SCA6). Various cognitive deficits were documented in 21 cases (all patients except one SCA6). Impairments were predominantly seen in figural memory, visuoconstructive abilities and verbal fluency. In the literature, an early psychomotor delay is described in several children with EA2 and FHM1, whilst reports of cognitive and psychiatric findings from adult cases are scarce.ConclusionsNeuropsychiatric manifestations are common in episodic CACNA1A disorders. In the case of otherwise unexplained developmental delay and a positive family history, CACNA1A mutations should be considered in the differential diagnosis.
Patient and public engagement in research and health system decision making: A systematic review of evaluation tools
Authors: Boivin Antoine , L'Espérance Audrey , Gauvin François‐Pierre , Dumez Vincent , Macaulay Ann C. , Lehoux Pascale , Abelson Julia 
Published: July 2018

AbstractBackgroundPatient and public engagement is growing, but evaluative efforts remain limited. Reviews looking at evaluation tools for patient engagement in individual decision making do exist, but no similar articles in research and health systems have been published.ObjectiveSystematically review and appraise evaluation tools for patient and public engagement in research and health system decision making.MethodsWe searched literature published between January 1980 and February 2016. Electronic databases (Ovid MEDLINE, Embase, Cochrane Database of Systematic Reviews, CINAHL and PsycINFO) were consulted, as well as grey literature obtained through Google, subject‐matter experts, social media and engagement organization websites. Two independent reviewers appraised the evaluation tools based on 4 assessment criteria: scientific rigour, patient and public perspective, comprehensiveness and usability.ResultsIn total, 10 663 unique references were identified, 27 were included. Most of these tools were developed in the last decade and were designed to support improvement of engagement activities. Only 11% of tools were explicitly based on a literature review, and just 7% were tested for reliability. Patients and members of the public were involved in designing 56% of the tools, mainly in the piloting stage, and 18.5% of tools were designed to report evaluation results to patients and the public.ConclusionA growing number of evaluation tools are available to support patient and public engagement in research and health system decision making. However, the scientific rigour with which such evaluation tools are developed could be improved, as well as the level of patient and public engagement in their design and reporting.
Impact of processing parameters and post‐treatment on the shape accuracy of 3D‐printed baking dough
Authors: Yang Fan , Zhang Min , Fang Zhongxiang , Liu Yaping 
Published: July 2018

SummaryApart from the material compositions, processing parameters of a 3D printer also affect the modelling effect of printed samples. This paper presents the influence of processing parameters including filament diameter (2.10, 2.30, 2.50 mm), nozzle movement speed (20, 25, 30 mm/s), nozzle diameter (0.8, 1.5, 2.0 mm) and nozzle height (2.10, 2.40, 2.60 mm), as well as that of post‐process fast‐cooling (−65 °C; 0, 5, 10 min), on the rheological properties and geometric accuracy of a 3D‐printed food construct made of baking dough (BD). Results showed that the printed object whose shape best matched the target geometry could be obtained with the following processing parameters: filament diameter of 2.30 mm, nozzle movement speed of 25 mm/s, nozzle diameter of 2.0 mm and nozzle height of 2.40 mm during 3D printing, combined with fast‐cooling at −65 °C for more than 10 min after printing and before baking.
Different sides of the same conversation: Black and White partners differ in perceptions of interaction content
Authors: Olson Michael A. , Johnson Camille S. , Zabel Kevin L. , Phillips Joy E. 
Published: July 2018

AbstractA positive interracial interaction can create a foundation for friendships, improved intergroup attitudes and reduced prejudice. Recent research has demonstrated that what people talk about in important. Here, two studies expand the interaction content model of interracial interactions to reveal that Black and White Americans perceive interaction content in similar and different ways. As expected, Black and White participants evaluated conversation topics along the same three dimensions, but differed in their perceptions of specific conversation topics. These convergences and differences emerged for pre‐generated (Study 1) and self‐generated (Study 2) topics. Factor analyses revealed that conversation attributes similarly distilled to the predicted underlying content dimensions of intimacy, valence, and controversy for Black and White Americans. However, Black individuals found interaction content, in general, to be more controversial, race‐related, enjoyable, and predictable than White individuals. Although both groups found race‐related content more controversial, Black individuals were less bothered by discussing race and found race‐related topics to be more predictable and enjoyable to discuss. These findings supported the interaction content model which may provide a framework for future research on interracial interactions. We conclude with the importance of considering differences in perceptions of interaction content, as well as suggestions for how intergroup interaction research could benefit from systematically incorporating such content.
Migration distance affects stopover use but not travel speed: contrasting patterns between long‐ and short‐distance migrating ospreys
Authors: Monti Flavio , Grémillet David , Sforzi Andrea , Dominici Jean Marie , Bagur Rafel Triay , Navarro Antoni Muñoz , Fusani Leonida , Klaassen Raymond H. G. , Alerstam Thomas , Duriez Olivier 
Published: July 2018

The development of migratory behaviour is a continuous process which is not only determined by genes, but also moulded by individual differences based on life‐history variations occurring at each ontogenetic stage. Assessing consistency and plasticity in migratory traits between long distance (LDM) and short distance migratory (SDM) populations within the same species that may express dissimilarities in the leeway of annual schedules is essential to understand the evolution and ontogeny of migratory strategies.We studied the migration strategies in autumn regarding flight speed and the use of stopovers (number and duration of stop‐overs across the whole journey) at the intra‐specific level, by tracking with GPS loggers the intercontinental migration of 43 adult and juvenile ospreys Pandion haliaetus from both LDM and SDM populations.LDM ospreys travelled distances five times larger than SDM ospreys, but their total migration speed was 2.4 times slower. While daily distance travelled did not differ between populations, the reduced total migration speed by LDMs was due to higher stopover use compared to SDM birds. SDM birds used more direct routes, crossing open sea at higher flight speeds, even though both populations largely benefitted from wind assistance across their journey. Across populations, adult birds travelled longer distances per day and displayed less sinuous migratory paths than juveniles, suggesting that migratory capabilities improve with age and experience of the bird. Overall, the time constraint related to total migration distance was not the main driver of the total migration speed, and other factors such as physiological needs to rest and refuel at stopover sites may play an important role. Our study underlines the importance of investigating variability in migration strategies in partially migratory species, for a better understanding of avian migratory ecology.
A systematic review of the use of ketogenic diets in adult patients with cancer
Authors: Sremanakova J. , Sowerbutts A. M. , Burden S. 
Published: July 2018

AbstractBackgroundA growing body of evidence indicates the importance of nutrition in cancer treatment. Ketogenic diets are one strategy that has been proposed to enhance traditional anticancer therapy. This review summarises the evidence concerning the effect of oral ketogenic diets on anthropometry, metabolism, quality of life (QoL) and tumour effects, at the same time as documenting adverse events and adherence in patients with cancer.MethodsWe searched electronic databases using medical subject headings (MeSH) and text words related to ketogenic diets and cancer. Adult patients following a ketogenic diet as a complementary therapy prior, alongside or after standard anticancer treatment for more than 7 days were included. Studies were assessed for quality using the Critical Appraisal Skills Programme tools (https://www.casp-uk.net).ResultsEleven studies were included with 102 participants (age range 34–87 years) from early‐phase trials, cohort studies and case reports. Studies included participants with brain, rectal or mixed cancer sites at an early or advanced disease stage. The duration of intervention ranged from 2.4 to 134.7 weeks (0.5–31 months). Evidence was inconclusive for nutritional status and adverse events. Mixed results were observed for blood parameters, tumour effects and QoL. Adherence to diet was low (50 out of 102; 49%) and ranged from 23.5% to 100%.ConclusionsHigh‐quality evidence on the effect of ketogenic diets on anthropometry, metabolism, QoL and tumour effects is currently lacking in oncology patients. Heterogeneity between studies and low adherence to diet affects the current evidence. There is an obvious gap in the evidence, highlighting the need for controlled trials to fully evaluate the intervention.
Vaccinia‐related kinase 2 modulates role of dysbindin by regulating protein stability
Authors: Jeong Young‐Hun , Choi Jung‐Hyun , Lee Dohyun , Kim Sangjune , Kim Kyong‐Tai 
Published: July 2018

AbstractVaccinia‐related kinase 2 (VRK2) is a serine/threonine kinase that belongs to the casein kinase 1 family. VRK2 has long been known for its relationship with neurodegenerative disorders such as schizophrenia. However, the role of VRK2 and the substrates associated with it are unknown. Dysbindin is known as one of the strong risk factors for schizophrenia. The expression of dysbindin is indeed significantly reduced in schizophrenia patients. Moreover, dysbindin is involved in neurite outgrowth and regulation of NMDA receptor signaling. Here, we first identified dysbindin as a novel interacting protein of VRK2 through immunoprecipitation. We hypothesized that dysbindin is phosphorylated by VRK2 and further that this phosphorylation plays an important role in the function of dysbindin. We show that VRK2 phosphorylates Ser 297 and Ser 299 of dysbindin using in vitro kinase assay. In addition, we found that VRK2‐mediated phosphorylation of dysbindin enhanced ubiquitination of dysbindin and consequently resulted in the decrease in its protein stability through western blotting. Over‐expression of VRK2 in human neuroblastoma (SH‐SY5Y) cells reduced neurite outgrowth induced by retinoic acid. Furthermore, a phosphomimetic mutant of dysbindin alleviated neurite outgrowth and affected surface expression of N‐methyl‐d‐aspartate 2A, a subunit of NMDA receptor in mouse hippocampal neurons. Together, our work reveals the regulation of dysbindin by VRK2, providing the association of these two proteins, which are commonly implicated in schizophrenia.Open science badgesThis article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at https://cos.io/our-services/open-science-badges/.
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