bluuebunny/crossref_metadata_2025_split huggingface dataset:
Topologic Analysis of Plasma Mitochondrial DNA Reveals the Coexistence of Both Linear and Circular Molecules
Authors: Ma Mary-Jane L , Zhang Haiqiang , Jiang Peiyong , Sin Sarah T K , Lam Wai Kei Jacky , Cheng Suk Hang , Lee Wing-Shan , Gai Wanxia , Tse O Y Olivia , Peng Wenlei , Wong John , Raghupathy Radha , Wong Raymond S M , Sahota Daljit , Leung Tak Y , Chan K C Allen , Chiu Rossa W K , Lo Y M Dennis 
Published: July 2019

Abstract

BACKGROUND
Cellular mitochondrial DNA (mtDNA) is organized as circular, covalently closed and double-stranded DNA. Studies have demonstrated the presence of short mtDNA fragments in plasma. It is not known whether circular mtDNA might concurrently exist with linear mtDNA in plasma.

METHODS
We elucidated the topology of plasma mtDNA using restriction enzyme BfaI cleavage signatures on mtDNA fragment ends to differentiate linear and circular mtDNA. mtDNA fragments with both ends carrying BfaI cleavage signatures were defined as circular-derived mtDNA, whereas those with no cleavage signature or with 1 cleavage signature were defined as linear-derived mtDNA. An independent assay using exonuclease V to remove linear DNA followed by restriction enzyme MspI digestion was used for confirming the conclusions based on BfaI cleavage analysis. We analyzed the presence of BfaI cleavage signatures on plasma DNA ends in nonhematopoietically and hematopoietically derived DNA molecules by sequencing plasma DNA of patients with liver transplantation and bone marrow transplantation.

RESULTS
Both linear and circular mtDNA coexisted in plasma. In patients with liver transplantation, donor-derived (i.e., liver) mtDNA molecules were mainly linear (median fraction, 91%; range, 75%–97%), whereas recipient-derived (i.e., hematopoietic) mtDNA molecules were mainly circular (median fraction, 88%; range, 77%–93%). The proportion of linear mtDNA was well correlated with liver DNA contribution in the plasma DNA pool (r = 0.83; P value = 0.0008). Consistent data were obtained from a bone marrow transplantation recipient in whom the donor-derived (i.e., hematopoietic) mtDNA molecules were predominantly circular.

CONCLUSIONS
Linear and circular mtDNA molecules coexist in plasma and may have different tissue origins.
CHEMICAL ELEMENT COMPOSITION OF HYPERICUM PERFORATUM PLANTS: ELEMENTS WHICH CONCEN-TRATIONS ARE NOT REGULATED
Authors: Siromlya Tat'yana Ivanovna , Zagurskaya Yuliya Vasil'yevna 
Published: July 2019

Hypericum perforatum L. (St. John’s wort) is a popular medicinal plant, but its chemical element composition has been studied insufficiently, especially of the plants originated in the Russian Federation. The aim of the study was to examine chemical element composition of Hypericum perforatum L plants originating from the south of West Siberia and to review similar data on plants grown in other climatic zones and regions, attempting to establish chemical elements' ranges in the phytomass of the studied species. Chemical element concentrations were determined by AES in 100 samples of aboveground and 60 samples of belowground H. perforatum plant parts collected in West Siberia (Novosibirsk and Kemerovo regions, the Altai Republic). Total concentrations of various chemical elements did not differ (P≤0.05) in different samples. The aboveground plant parts were found to have very high concentrations of K, Са, P, Si, Mg (n×103–104 mg/kg) alongside with high concentrations of Al, Fe, Na (n×102 mg/kg) and moderate concentrations of Мn, Sr, Ba, Zn, B, Ti, Cu (n×10 mg/kg), whereas such elements as Ni, Zr (n mg/kg) were found in decreased concentrations, while V, Cr, Mo, Co, Y, Ga showed low concentrations (n×10-1 mg/kg), Sc, Ве, Yb (n×10-2 mg/kg) being very low. The accumulation of Ba and Sr in plants revealed some regional peculiarities as their concentrations in plants of Siberian origin was higher than in plants of the European one. The average chemical element concentrations for a wide set of world data displayed a wider range, with extremely high or low values, as compared to the regional range of variation. The studied plants had no barriers for the uptake of Zn, Р, B, Mg, Mn and K, but for Са, Ва, Sr, Mo, Co in different samples the root barrier coefficient was higher, lower or equal to 1.
SECONDARY PLANT METABOLITES ACHILLEA MILLEFOLIUM AND GOSSYPIUM HIRSUTUM L. AND THEIR BIOLOGICAL EFFECTIVENESS AGAINST THE RED SPIDER MITE
Authors: Zakirova Rano Pulatovna , Khidirova Nazira Kudratovna , Eshbakova Komila Alibekovna , Melieva Shohista Olimovna , Urakov Barat Abdubakievich 
Published: July 2019

The biological activity of secondary metabolites with respect to pest insects is a poorly studied field of scientific research and opens wide possibilities for revealing many aspects of the theoretical and practical plan.
The aim of the work is to create a plant protection product against the effects of a red spider mite of complex action on the basis of secondary metabolites of yarrow Achillea millefolium (Asteraceae) and cotton Gossypium hirsutum L. (Malvaceae).
The chemical composition of the aerial part of the A. millefolium plant collected in the foothill areas of the Tashkent region of the Republic of Uzbekistan was studied. A high yield of eucalyptol (1,8-cineol) – 37.71% of the amount of the extract was revealed by chromatography-mass spectrometry. The method of high-performance thin layer chromatography revealed that the main component of Uchkun's preparation was the sum of polyprenyl homologs with 10–12 isoprene units, the content of which reached 69.5%. Other components of the extract were phytosiosterol and tocopherols in an amount of 30.5%. A synergistic effect was established in the combination of monoterpenoids of the yarrow plant of ordinary and polyprenols of Uchkun. The composition becomes more effective, having both a toxic effect on the red spider mite and further improving the state of the photosynthetic apparatus in damaged leaves of cucumber plants. As a result of the work carried out, it is shown that with a degree of infection of plants within the range of 100–150 pieces per sheet, the developed composition can replace the treatment with a chemical protective agent – a drug based on abamectin – Vertimec.
HYDROGENATION OF ABIES WOOD AND ETHANOL-LIGNIN BY MOLECULAR HYDROGEN IN SUPERCRITI-CAL ETHANOL OVER BIFUNCTIONAL RU/C CATALYST
Authors: Kazachenko Aleksandr Sergeyevich , Baryshnikov Sergey Viktorovich , Chudina Anna Il'inichna , Malyar Yuriy Nikolayevich , Sychev Valentin Vladimirovich , Taran Oksana Pavlovna , Djakovitch Laurent , Kuznetsov Boris Nikolayevich 
Published: July 2019

The effect of a bifunctional catalyst containing nano-dispersed ruthenium particles on an oxidized carbon support “Sibunit” on the yield and composition of products in the processes of hydrogenation of abies wood and abies ethanol-lignin in a supercritical ethanol medium at 250° C was studied. The presence of Ru/C catalyst resulted in a raise the ethanol-lignin conversion from 85 to 98 wt.%, the yield of liquid product from 75 to 85 wt.%, the yield of gases – by 1.5 times, but the yield of solid products dropped from 14 to 2.8 wt.%.
Ruthenium catalyst increased the conversion of abies wood by 12.5 wt.%, but did not affect the yield of liquid products. The yield of the solid residue in the presence of the catalyst was reduced by 12.5 wt.%, and the yield of gases rose by 2.5 times.
Simultaneously, the ruthenium catalyst promoted the process of hydrodeoxygenation of liquid products, diminution their average molecular mass from 1174 g/mol to 827 g/mol and the formation of monomeric and dimeric compounds with a molecular mass 193 and 426 g/mol, respectively. The solid residue of catalytic hydrogenation contained 70.1 wt.% of cellulose. Thus, the application of Ru/C catalyst in the process of hydrogenation of abies wood allowed the reductive fractionation of wood biomass into a solid cellulose product, liquid and gaseous products from lignin and hemicelluloses.
BRICS ÜLKELERİ İLE TÜRKİYE’NİN PERFORMANS VE POTANSİYEL KRİTERLERİ ÇERÇEVESİNDE DEĞERLENDİRİLMESİ
Authors: BATTAL Turgay , AKAN Ercan 
Published: July 2019

BRICS ülkeleri Dünya nüfusunun yüzde 43’ünü, Dünya ekonomisinin de yüzde 30’unu kapsamasından dolayı önem arz etmektedir. Türkiye son yıllarda başardığı dönüşüm hamleleri ile gelişme gösteren bir ülke olarak BRICS’e üye olabileceğini en üst mertebeden dile getirmiştir. Literatürde BRICS ile ilgili birçok çalışma olmasına rağmen, içinde Türkiye’nin de olduğu, ekonomik ve lojistik performanslarıyla, ülkelerin potansiyellerini ölçmeye yönelik çalışmaların oldukça kısıtlı olduğu görülmektedir. Yapılan bu çalışma ülkelerin ekonomik performanslarını ölçen, yaygın olarak kullanılan makroekonomik kriterlerin yanı sıra ülkelerin gelişiminde önemli rol oynayan lojistik performans kriterleri de kullanılmıştır. Bu kriterlere ilave olarak ülkelerin gelişme potansiyellerini ölçmeye katkı sağlayabilecek özgün kriterler seçilerek ilgili ülkelerin gelişme potansiyelleri de bu çalışmada dikkate alınmıştır. Genel olarak ülkelerin değerlendirilmesi, ülkelerin ilgili başlıklardaki verilerinin birincil veya ikincil kaynaklardan toplanarak çeşitli kalitatif ve kantitatif metotlar ile karşılaştırılarak sıralanması yoluyla yapılmaktadır. Çok Kriterli Karar Verme Yöntemleri (ÇKKV) bu tür karşılaştırmalarda kullanılan ve elde edilen sonuçların alternatifleri arasında objektif olarak değerlendirilmesine imkân vermesi sebebiyle araştırmalarda sıklıkla kullanılmaktadır. Bu çalışmada kriterlerin CRITIC yöntemiyle ağırlıklandırılması ile hesaplanması ve PROMTHEE-GAIA ÇKKV yöntemi ile sıralanması ve çıkan sonuçların değerlendirilmesi yoluyla yapılmış özgün bir çalışmadır.
TARIMSAL ÜRÜN FİYAT ARTIŞLARININ TÜRKİYE TARIMININ YAPISAL SORUNLARINA DAİR GÖSTERDİKLERİ
Authors: ÖNAL Nevzat Evrim 
Published: July 2019

Türkiye’de işlenmemiş tarımsal ürünlerin perakende fiyatları Kasım 2018 itibariyle genel olarak zaten yüksek seyretmekte olan enflasyonun çok ötesinde bir artış sürecine girdi. Yaş sebze ve meyvede dört ay zarfında yüzde 50’yi aşan nominal fiyat artışı doğal olarak hararetli tartışmaları da tetikledi ve meseleye çok sayıda açıklama getirildi. Bu açıklamalar içerisinde en yaygını Türkiye tarımının girdi açısından dışa bağımlılığı ve döviz kurunda 2018 ortasında yaşanan şokun fiyatlara yansımış olmasıydı. Bu açıklama, belli bir düzeyde doğru olsa da; bu makalenin yazarı döviz kuru ile işlenmemiş tarımsal ürünlerin perakende fiyatları arasında kurulan ilişkinin Türkiye’nin tarımsal ürün ihracatı dikkate alınmadığı müddetçe eksikli olacağını düşünmektedir. Fiyatların kura verdiği şiddetli tepki, Türkiye tarımının son yirmi yılını kapsayan yapısal sorunlarının bir bakiyesi niteliğindedir ve doğru yöntem, olanların bu tarihsel perspektiften değerlendirilmesidir. Dolayısıyla bu makalede, son verilerin işaret ettiği eğilimler tarihsel analize tabi tutulacak ve eldeki soruna kısa vadeli ve geçici değil; uzun vadeli ve (en azından daha) kalıcı, bütünlüklü bir çözüm önerilecektir.
Application of patient-specific 3D navigation templates for pedicle screw fixation of subaxial and upper thoracic vertebrae
Authors: Kovalenko R. A. , Rudenko V. V. , Kashin V. A. , Cherebillo V. Yu. , Ptashnikov D. A. 
Published: July 2019

Objective. To analyze the safety and accuracy of pedicle screw placement in the subaxial cervical and upper thoracic spine using patient-specific 3D navigation templates.Material and Methods. The study included 16 patients who underwent transpedicular implantation of screws in the subaxial cervical and upper thoracic vertebrae using patient-specific 3D navigation templates. A total of 88 screws were installed. All patients underwent preoperative CT angiography to assess visualization of the vertebral artery. Customized vertebral models and navigation templates were created using 3D printing technology. Models and templates were sterilized and used during surgery. The results of screw implantation, as well as the safety and accuracy of the placement, were assessed by postoperative CT.Results. The average deviation from the planned trajectory was 1.8 ± 0.9 mm. Deviation was estimated as class 1 (<2 mm) for 57 (64.77 %) screws, class 2 (2–4 mm) for 29 (32.95 %), and class 3 for two (2.27 %). The safety of screw implantation of grade 0 (the screw is completely inside the bone structure) was in 79 (89.77 %) cases, of grade 1 (<50 % of the screw diameter perforates the bone) – in 5 (5.68 %), and of grade 3 – in 2 (2.27 %).Conclusion. Using 3D navigation templates is an affordable and safe method of installing pedicle screws in the cervical and upper thoracic spine. The method can be used as an alternative to intraoperative CT navigation.
Personal resources of protective-coping behavior of adolescents with idiopathic scoliosis at the stage of preparation for surgery
Authors: Pyatakova G. V. , Kozhevnikova A. O. , Vissarionov S. V. 
Published: July 2019

Objective. To analyze personal resources in adolescents with idiopathic scoliosis at the stage of preparation for surgery.Material and Methods. The study involved 29 adolescents aged 12–17 years (7 boys and 22 girls) with grade III–IV idiopathic scoliosis. The survey was conducted within the first days of admission. The control group consisted of 30 adolescents aged 12–17 years without serious somatic diseases. The following diagnostic methods were used: analysis of medical records (case histories), semi-structured interview to assess the severity of traumatic experiences in children and adolescents, the Life Style Index questionnaire, the Coping Strategies Questionnaire, a self-assessment scale, and self-test questionnaire.Results. In adolescents with idiopathic scoliosis, events associated with the disease and preparation for surgery are accompanied by extreme level experiences manifested in medium and medium-high values of post-traumatic stress. Moderate symptoms of avoidance, hyperarousal and obsessive reproduction of traumatic episodes predominate in the structure of stress. Positive self-attitude indicators contribute to the activation of effective coping strategies and can be considered as an adaptive personal resource in a difficult life situation associated with a serious illness and the upcoming surgery.Conclusion. Understanding of personal resources that contribute to the formation of effective coping behavior in a difficult life situation associated with the disease and complex surgical treatment will optimize psychological preparation of adolescents with idiopathic scoliosis for surgery.
The Construction of National Identity through Cybernetic Process: The Example of “K’na” Dance Event in Greek and Turkish Thrace
Authors: Filippidou Eleni , Koutsouba Maria , Lalioti Vassiliki , Lantzos Vassilis 
Published: July 2019

Abstract
The research field of this paper is the area of Thrace, a large geopolitical-cultural unit that was divided – due to political reasons – in three subareas distributed among three different countries: Bulgaria, Turkey and Greece. A dance event that used to take place before the border demarcation but is still performed in the Greek and Turkish Thrace is that of “K’na”, a wedding dance event danced by the people of both border areas, despite of the changes in their magical-religious beliefs and the changes brought by socio-economic and cultural development. In particular, the aim of this paper is the study of the “construction” of the national identity of inhabitants both of Greek and Turkish Thrace, as this is manifested through the dance practice within the wedding event of “K’na”, through the lens of sociocybernetics. Data was gathered through ethnographic method as this is applied to the study of dance, while its interpretation was based on sociocybernetics according to Burke’s identity control theory. From the data analysis, it is showed that the “K’na” dance in Greek and Turkish Thrace constructs and reconstructs the national identity of the people who use them as a response to the messages they receive via the communication with “the national others”. In conclusion, the “construction” of the identity results from a continuous procedure of self-regulation and self-control through a cybernetic sequence of steps.
Achilles Tendinopathy: An Evidence-Based Overview for the Sports Medicine Professional
Authors: Kaplan Kelby , Olivencia Ovidio , Dreger Melissa , Hanney William J. , Kolber Morey J. 
Published: July 2019

ABSTRACTACHILLES TENDINOPATHY (AT) IS A CONDITION WHEREBY INDIVIDUALS EXPERIENCE PAIN AND IMPAIRMENTS AS A RESULT OF PATHOLOGICAL CHANGES AT THE ACHILLES TENDON AND NEIGHBORING TISSUES. THIS ARTICLE PROVIDES AN EVIDENCE-BASED OVERVIEW OF THE STRUCTURAL PATHOLOGY AND CLINICAL SEQUELA ASSOCIATED WITH AT. THE EVIDENCE UNDERPINNING MORE COMMON TREATMENTS, WITH AN EMPHASIS ON EXERCISE INTERVENTIONS, IS PRESENTED IN AN EFFORT TO MITIGATE THE IMPAIRMENT SEQUELA AND GUIDE SPORTS MEDICINE PROFESSIONALS IN THEIR CHOICE OF TREATMENTS FOR AT.
Plantação de Bananeira - A atentividade e a imediação como procedimentos de estudo da dança na Escola Básica Municipal Victor Miguel (Florianópolis – SC)
Authors: Ferro Felipe Ferreira , Da Silva Heloísa Marina 
Published: July 2019

Este artigo analisa a proposição Plantação de Bananeira, um projeto articulado com práticas e pesquisas que ocorreram em Florianópolis desde 2017, em escolas públicas. Com atenção à prática da improvisação na dança, o estudo tem apetite por Pedagogias Radicais, arte relacional e atentividade na performance. Além disso, Plantação de Bananeira abre o caminho para a investigação de formas de participação e condução de processos criativos, onde eu elaborei o eixo: o que é a imediação no processo de aprendizagem da dança?A imediação pode ser como apontar um outro caminho onde o sensível, a polifonia dos afetos engendrados na experiência, ou situações em que a diversidade prevalece e nos chama a criar a partir dela, em vez de reiterar os parâmetros regulatórios inerentes ao regime normativo da instituição escolar. Essa preocupação surge a partir do objetivo de criar campos para o estudo da dança em sua verve atual e relacional, para que possamos nos conectar com poderes ainda não atualizados de forma dançada, conectando o que chamamos de memória do passado com a vida em que nós gostaríamos de ser coletivamente. Plantar Bananeira é uma proposta de reativação do ânima para que a vida seja criada em outros campos de diversidade.
Sex Differences in Cognitive Flexibility and Resting Brain Networks in Middle-Aged Marmosets
Authors: LaClair M. , Febo M. , Nephew B. , Gervais N.J. , Poirier G. , Workman K. , Chumachenko S. , Payne L. , Moore M.C. , King J.A. , Lacreuse A. 
Published: July 2019

AbstractSex differences in human cognitive performance are well characterized. However, the neural correlates of these differences remain elusive. This issue may be clarified using nonhuman primates, for which sociocultural influences are minimized. We used the marmoset (Callithrix jacchus) to investigate sex differences in two aspects of executive function: reversal learning and intradimensional/extradimensional (ID/ED) set shifting. Stress reactivity and motor function were also assessed. In agreement with human literature, females needed more trials than males to acquire the reversals. No sex differences in ED set shifting or motivational measures were observed. The findings suggest enhanced habit formation in females, perhaps due to striatal estrogenic effects. Both sexes showed increased urinary cortisol during social separation stressor, but females showed an earlier increase in cortisol and a greater increase in agitated locomotion, possibly indicating enhanced stress reactivity. Independent of sex, basal cortisol predicted cognitive performance. No sex differences were found in motor performance. Associations between brain networks and reversal learning performance were investigated using resting state fMRI. Resting state functional connectivity (rsFC) analyses revealed sex differences in cognitive networks, with differences in overall neural network metrics and specific regions, including the prefrontal cortex, caudate, putamen, and nucleus accumbens. Correlations between cognitive flexibility and neural connectivity indicate that sex differences in cognitive flexibility are related to sex-dependent patterns of resting brain networks. Overall, our findings reveal sex differences in reversal learning, brain networks, and their relationship in the marmoset, positioning this species as an excellent model to investigate the biological basis of cognitive sex differences.
Centella asiatica prevents chronic unpredictable mild stress-induced behavioral changes in rats
Authors: Jagadeesan Saravanan , Musa Chiroma Samaila , Baharuldin Mohamad Taufik Hidayat , Taib Che Norma Mat , Amom Zulkhairi , Adenan Mohd Ilham , Moklas Mohamad Aris Mohd 
Published: July 2019

Background: Depression is a psychological disorder which is associated with mood swings and cognitive deficits, and is ranked third among the leading causes of global health care burden. Chronic unpredictable mild stress (CUMS) has been shown to induce depression-like behaviors in rodents, which exhibit similarities to the human form of depression. Centella asiatica (CA) is an ancient medicinal plant which has neuroprotective potential. This work aimed to assess the protective role of CA on CUMS-induced rats.
Methods: Thirty six rats randomly divided into six groups were used for the evaluation. The groups were as follows: 1, Unstressed + normal saline (control); 2, CUMS + normal saline (model); 3, CUMS + fluoxetine (10 mg/kg) standard antidepressant; 4-6, CUMS + CA (200, 400 & 800 mg/kg). Nine different stressors (S) (S1: 24-h food deprivation, S2: 24- h water deprivation, S3: 5-min cold swimming (at 5◦C), S4: 12-h change of cage mate, S5: 1-min tail pinch (1 cm from the tip of the tail), S6: 12-h cage tilt (at 45◦), S7: 12-h overcrowding of cage, S8: 12-h wet bedding with 200 mL of water, and S9: 4-h physical restraint) were administered to the stressed groups with at least 2 stressors per day. The treatments lasted for 8 weeks, and the rats were evaluated through open field test (OFT) and elevated plus maze (EPM) for anxiety-like behavior, forced swimming test (FST) for depression-like behaviour, and T-maze spontaneous alternation for learning and memory. Furthermore, serum cortisol levels were also evaluated.
Results: CUMS-induced rats showed anxiety-like behaviors in OFT and EPM tests, depression-like behavior in FST and cognitive deficits in T-maze test, as well as increased serum cortisol levels. Conversely, administration of CA (at 400 and 800 mg/kg doses) and fluoxetine (at 10 mg/kg dose) prevented the aberrant behavioral changes and also the changes in the serum cortisol levels. No significant differences of behaviors were observed between the groups of rats administered with CA (400 and 800 mg/kg) and those administered with fluoxetine (10 mg/kg), suggesting that the therapeutic potential of CA is comparable to that of fluoxetine.
Conclusion: The data obtained showed that CA, at doses of 400 and 800 mg/kg, effectively reversed the anxiety and depression-like behaviors, amnesic behaviors, as well as serum cortisol levels in a CUMS-induced rat model of depression. This suggests that CA is a potential candidate for the development of future anti-depressants.