bluuebunny/crossref_metadata_2025_split huggingface dataset:
EGFR mutation: a prognostic factor associated with immune infiltration in lower-grade glioma.
Authors: Hao Zhaonian , Guo Dongsheng 
Published: September 2019

Abstract
Glioma is one of the most common type of primary central nervous system (CNS) tumors. EGFR mutation, a common alteration occurs in various tumors, is not brought to the forefront in understanding and treating glioma at present. In the present study, we demonstrated an immune infiltration related pattern of EGFR mutation in lower-grade glioma. In silico analyses were performed to investigate EGFR mutation and its biological effects and clinical values. GO and GSEA process were used as enrichment analysis. Infiltration levels of specific types of immune cells were estimated at TIMER database. Clinical data of patients were obtained from TCGA and were employed for survival analyses. Results revealed that EGFR mutation leads to an up-regulation of immune response related pathways and dismal prognosis in lower-grade glioma. Infiltration of CD4+ T cells, neutrophils, macrophages, and dendritic cells were significantly increased in EGFR-mutant cases. Infiltration of specific types of immune cells were correlated with shorter survival time. PD-L1 was elevated in EGFR-mutant cases and correlated with infiltration level of CD4+ T cells, neutrophils and dendritic cells. In conclusion, EGFR mutation indicates increasing infiltration of specific types of immune cells and poor prognosis in lower-grade glioma. Alteration of immune microenvironment since the EGFR mutation might influence the survival of glioma. We also provided a novel evidence and indicator of PD-1 inhibitor application in glioma.
Young-Onset Colorectal Cancer in the North East of Scotland: Survival, Clinico-Pathological Features and Genetics
Authors: Perrott Sarah , Laurie Kirsten , Laws Kirsten , Johnes Annie , Miedzybrodzka Zosia , Samuel Leslie 
Published: September 2019

Abstract
Background
Colorectal cancer (CRC) in patients aged under 55 years is on the rise, constituting approximately 10% of cases. Our aim was to determine the survival and clinico-pathological details of young-onset CRC (yCRC), as well as audit the referral rate to genetic services and thus establish the incidence of microsatellite instability (MSI).
Methods
A retrospective case note review was conducted for patients aged under 55 years who were diagnosed with CRC between 2005-2015 in the North East of Scotland. Cases were identified by pathology records and data was obtained from patient notes then crosschecked with the NHS Grampian genetics electronic case records. Analysis was performed using SPSS version 25 (IBM, New York, USA) to produce Kaplan-Meier survival estimates, descriptive statistics and markers predictive for genetic referral.
Results
Data from 345 patients (age range 22-54 years) were analysed. The one year, five year and overall survival rates were found to be 89%, 63% and 55%, respectively. A majority of patients (61%) presented with advanced Dukes’ C or D disease. Of 201 patients that met criteria for genetic referral, only 93 (46%) were referred to genetics services. MSI was identified in 14% of those referred.
Conclusions
Survival in yCRC was found to be better than that in later onset disease, despite higher rates of advanced disease. Patients were under-referred to genetic services, where a significant proportion were found to be MSI positive.
RHEALITY: Recurrence of HEr2+ pAtients: evaluation of Long term outcome In patients receiving trastuzumab TherapY
Authors: Saghatchian Mahasti , Curtit Elsa , Coeffic David , Flinois Alain , Levy Christelle 
Published: September 2019

Abstract
Background Despite improved prognosis of HER2+ eBC since the introduction of trastuzumab in the adjuvant setting in 2006, disease recurrences still occur in some patients after a few years. We aimed to describe in real life the long-term follow-up to assess disease-free and metastasis-free survival of patients with HER2+ eBC treated with adjuvant trastuzumab. Methods This was a multicenter, retrospective, cohort study assessing HER2+ eBC patients diagnosed between 2009 and 2010 and treated with adjuvant trastuzumab. Data were collected from patient’s medical charts. Disease-free survival (DFS), and metastatic-free survival (MFS) were evaluated in the overall population and within subgroups according to hormonal and nodal status. Results In the overall population (n=2,513) at 7 years, the DFS rate was 75.8% [95%CI 74.0%-77.6%], and the MFS rate was 84.1% [82.5%-85.6%]. According to hormonal status, the 7-year DFS rate was significantly higher for HR+ than for HR- patients [80.0% vs . 68.6%; p <0.001], and the 7-year MFS rate [87.9% for HR+ vs . 77.5% HR-]. According to nodal status, the 7-year DFS rate was significantly higher for N- than for N+ patients [86.7% vs . 66.4%; p <0.001], and the 7-year MFS rate [94.7% for N- vs . 74.9% for N+]. Conclusions Despite introduction of adjuvant trastuzumab, prognosis of HER2+ eBC is still a matter above all in subgroups associated with a higher risk of disease recurrence. Our real-world study pointed out a particularly aggressive profile of N+ and HR- subgroups and the need for more efficient approaches for these particular group of patients.
Characteristics of gas-forming pyogenic liver abscess caused by Klebsiella pneumoniae and Clostridi perfringens
Authors: Chen Zheng-Yang , Jiang Nan , Guo Song , Li Bin-Bin , Yang Jia-Qi , Chai Shao-Bin , Yan Hong-Feng , Yang He-Ming , Zhou Jin-Lian , Sun Hong-Wei , Cui Yan 
Published: September 2019

Abstract
Background: Gas-forming pyogenic liver abscess is a life-threatening disease with poor prognosis commonly caused by 2 bacteria, Klebsiella pneumoniae and Clostridium perfringens . Due to its low incidence and associated high mortality rate, it is important to study the biological characteristics of the disease. The aim of this study was to conduct a worldwide review of literature on gas-forming pyogenic liver abscess caused by K. pneumoniae and C. perfringens .
Methods: We searched PubMed and Web of Science databases from January 2009 to March 2019, with published in English. All relevant articles were accessed in full text. The manual search included references of retrieved articles. Finally, 35 publications were selected for review.
Results: The results showed that more cases of gas-forming pyogenic liver abscess in Asia were caused by K. pneumoniae than by C. perfringens (P=0.011). The prevalence of diabetes mellitus in patients with gas-forming pyogenic liver abscess caused by K. pneumoniae was higher than caused by C. perfringens (P=0.032). The survival rate of patients with gas-forming pyogenic liver abscess caused by K. pneumoniae who received surgical debridement or drainage was higher than caused by C. perfringens (P=0.002).
Conclusions: The prevalence of diabetes mellitus was higher in patients with gas-forming pyogenic liver abscess caused by K. pneumoniae than in patients caused by C. perfringens .
Historical changes in recurrence forms and their mechanisms after laparoscopic transabdominal preperitoneal inguinal hernioplasty, as assessed by video review
Authors: Sato Masanori , Nozawa Masashi , Watanabe Takahiro , Onoda Takanobu , Matsuyama Atsuko , Shiiya Norihiko , Wada Hidetoshi 
Published: September 2019

Abstract
Background: Technical factors leading to hernia recurrence after transabdominal preperitoneal (TAPP) repair include insufficient dissection, inadequate prosthetic overlap and prosthetic size, improper fixation and folding or crinkling of the prosthesis. However, which of these can intraoperatively determine if a case will go on to develop recurrent hernias remains unclear. Methods: We reviewed operation videos for 27 control cases and primary laparoscopic hernioplasty of 13 lesions where the patient suffered recurrence after TAPP repair. Five surgeons blindly reviewed the videos to assess surgical techniques such as adequate dissection, mesh coverage, and folding or crinkling of the mesh. We explored the association of the technique with development of recurrence across our policy change, and hernia type before and after recurrence. Results: After implementing a TAPP repair policy of covering myopectineal orifice (MPO) with sufficiently overlapped mesh, 79% of the rating were satisfactory, whereas 34% were satisfactory prior to the policy. The recurrence rate decreased to 0.7% (5/678) compared with 6.2% (10/161) before the policy ( p <0.001). Although various types of recurrences were noted in the earlier period, only primary indirect and recurrent indirect hernias were observed in the later period ( p =0.006). With univariate analysis, the scores of posterior prosthesis overlap to the MPO in the recurrent cases were significantly lower than controls in the later period ( p =0.019). Conclusions: Fully covering the MPO with mesh is essential for preventing direct type recurrence. Prosthesis overlap posterior to the MPO in a large indirect hernia is important for preventing recurrence.
Neonatal hand, foot, and mouth disease: a case-control study in Shanghai
Authors: Xu Shanshan , Li Huajun , Qiao Peng , Xu Guofeng , Zhao Dongying , Lin Xiaoyan , Qin Yu , Yu Huiju , Zhang Xi , Zhang Wanju , Huang Lisu 
Published: September 2019

Abstract
Background Evidence of hand, foot, and mouth disease (HFMD) in neonates is limited. This study aimed to report the clinical symptoms, possible transmission routes, and prognosis of neonatal HFMD in Shanghai. Methods This was a case-control study based on registry system of HFMD. All neonates and infected family members were enrolled between 2016 and 2017 in Shanghai. Neonates with HFMD were followed for at least half a year. The detailed questionnaires, medical history and physical examination were recorded. Routine blood examination, liver and renal function, immunophenotypes of peripheral blood lymphocytes (CD3, CD4, and CD8 T-cells; NK cells), immunoglobulin (Ig) M, IgG, and IgA and cytokine interleukin (IL-1β, IL-2R, IL-6, IL-8, IL-10, and TNF-α) levels were detected. All rectal swab specimens were collected and genotyped for enterovirus. T-test or nonparametric test was used to evaluate the differences. Logistic analysis was applied to find the risk of clinical symptoms in the group of neonates and their HFMD paired siblings.Results There were 16 neonates among the 12608 diagnosed patients with HFMD, accounting for 1.3‰. All the neonates were transmitted within-family, mainly by the elder sibling, with different types of coxsackievirus A6 infection. Coxsackievirus A6 was also the emerging and predominant causative agent of HFMD in Shanghai. None of the neonates with HFMD suffered fever, onychomadesis, or severe complications. However, two elder sibling patients showed lethargy, and one developed hypoperfusion. The white blood cells in the elder siblings with HFMD were generally higher than the neonates with HFMD. The immunologic function of the neonates was basically normal. The inflammatory response was high regardless of the neonates or elder siblings. The clinical symptoms receded at about one week. None of the neonates had sequalae.Conclusions The severity of neonates infected with HFMD may vary with pathogen. With the two-child policy in China, it should be noticed that elder siblings may be the main route of HFMD transmission.
Seasonal variation of acute appendicitis and fecaliths in children
Authors: Hsu Yao-Jen , Fu Yu-Wei , Chin Taiwai 
Published: September 2019

Abstract
Purpose A higher incidence of acute appendicitis (AA) in summer has been reported. The reason of this observation is uncertain. The purpose of this study is to analyze the difference of the clinical findings between summer and non-summer AA.
Methods We reviewed the clinical data of 171 patients who had an appendectomy from 2013 to 2016. The patients were under 18 years of age by the time of surgery. Laboratory data, CT scans, pathologic reports and operative records were reviewed.
Results More appendectomies were performed in summer than non-summer months (101 patients vs 70 patients). There is no significant difference of laboratory results between the summer and non-summer patients. The percentage of AA patients with a fecalith is significantly lower in the summer (33.6%) than non-summer months (55.7%). There was no significant difference in appendiceal perforation/abscess between summer and non-summer months.
Conclusions We observed a lower percentage of AA with a fecalith in summer. To our knowledge, this observation has not been reported in literature. The increase of AA in summer may be a result of more lymphoid hyperplasia in summer. This may correlate with the yearly outbreak of enteroviral infections in this area.
Candida Coinfection among Patients with Pulmonary tuberculosis in the World; A Systematic Review and Meta-analysis of Cross-Sectional Studies
Authors: Ghazalibina Mehran , Shakerimoghaddam Ali , Khaledi Azad 
Published: September 2019

Abstract
Background

Diagnosis of fungal co-infections in patients with pulmonary tuberculosis has critical importance. In this review, we aimed to determine the prevalence of candida coinfection in patients with pulmonary tuberculosis.Methods

The present systematic review of cross-sectional studies was conducted based on the Preferred Reporting Items for Systematic reviews and Meta-analysis (PRISMA) Protocol. Studies published online in English from January 2001 to March 2019 were assessed. Literature search was performed in Web of Science, MEDLINE/PubMed, and Scopus databases using keywords combinations of “pulmonary fungal”, “pulmonary coinfection”, OR “pulmonary mycosis”, “pulmonary fungal infections/agents”, OR “polymicrobial infection”, OR “secondary infection”, OR “mixed infections”, “pulmonary candidiasis”, “fungi coinfection”, “fungal co-colonization”, AND “pulmonary tuberculosis”, OR “pulmonary TB”. Data was analyzed using Comprehensive Meta-Analysis software. Heterogeneity between studies was evaluated by Cochran's Q, and I 2 tests.Results

The pooled global prevalence of candida coinfection among patients with pulmonary tuberculosis was 25.7% (95% CI: 23.7-27.9). C. albicans was the most prevalent Candida spp. with a pooled prevalence of 65.8% (95% CI: 54.3-75.7). Risk factors of candida coinfection included smoking, diabetes, advanced age, and low body mass index.Conclusion

The present review showed the high rate of candida coinfection among patients suffering from pulmonary tuberculosis. Adequate measures are necessary to early diagnose and treat these infections.
Comparative analysis of different host adaptation in two rice planthopper species
Authors: Huang Hai-Jian , Cui Jia-Rong , Hong Xiao-Yue 
Published: September 2019

Abstract
Background The brown planthopper (Nilaparvata lugens, BPH) and small brown planthopper (Laodelphax striatellus, SBPH) are most destructive insect pests belonging to Delphacidae. These two species differ in host range, but the underlying mechanism remained unknown.Results In this work, we compared the gut transcriptome of two planthoppers that colonized on rice, colonized on wheat, and transferred from rice to wheat. We found that the majority of differentially expressed genes in SBPH were Type I responsive, which are plastic and evolved in the same direction. Genes associated with sugar transporters and heat shock proteins showed similar expression trends between BPH and SBPH. However, distinct gene expression variation were found after BPH and SBPH transferring from rice to wheat, with detoxifying-, ribosomal-, and amino acid metabolic-related genes reciprocally regulated.Conclusion The results of this study provide evidence that non-adaptation of BPH on wheat was determined within 24 h after transferring, with the majority of genes regulating in “wrong” way. In contrast, SBPH sophisticatedly response to wheat hosts, with the majority of genes plastic and evolved in same direction. Our study promotes our understanding on herbivore adapting to different food source, and might provide a potential strategy for pest management.
Relocation experiences of the elderly to a long-term care facility in Taiwan: a qualitative study
Authors: Wu Chia-Shan , Rong Jiin-Ru 
Published: September 2019

Abstract
Background Relocation to a long-term care (LTC) facility is a major life change for most elderly people. Following relocation, many elderly experience difficulties in adapting to changes in the living environment. Taiwan is increasingly becoming an "aging society” and Taiwanese elderly is shown to differ in their expectations of long-term residential care. However, in-depth evidence on the experiences of the elderly of their stay in LTC facilities in Taiwan is relatively sparse. The aim of this study was to explore the relocation experiences of the elderly to a LTC facility to inform policy and practice to address their needs effectively. Methods A qualitative study, using semi-structured in-depth interviews, was conducted to explore the experiences of 16 elderly people who have relocated to and lived in a LTC facility in Taiwan for up to a period of 12 months. All interviews were recorded, transcribed, and analyzed using grounded theory approach. Results Participants’ accounts reflected four interrelated key themes: wish to minimize the burden, but stay connected with the family; perceived barriers to adaptation; valuing tailored care; and acceptance and engagement. Each theme included interrelated subthemes that influenced one another and represented the different stages in the relocation journey. Most participants viewed relocation as a way of minimizing the burden of their care from family members, but desired to keep a close connection with family and friends. Participants recounted experiences of psychological resistance while making the decision to relocate. Fear of losing autonomy and the ability to perform self-care was a major reason for resistance to adapt. Provision of tailored care was accorded much value by the participants. The eventual decision to face the challenge and to adapt themselves to the new environment due to their needs for constant care was explicit in some accounts. Conclusions Relocation to LTC facility is a dynamic process in the first year of moving into the facility, and involves a range of emotions, feelings and experiences. Adaptation of the elderly into the LTC facility can be maximized if the relocation is well planned with provisions for individually tailored care and family involvement.
The role of Bacillus acidophilus in osteoporosis and its roles in osteocyte proliferation and differentiation
Authors: Chen Chen , Dong Baokang , Wang Yuming , Zhang Qiang , Wang Bangmao , Feng Shuzhi , Zhu Yu 
Published: September 2019

Abstract
The microbes play an important role to physiological activities of human. Osteoporosis is one of the most closely relationship diseases with the elderly. In recent years, the studies found that gut microbiota can cause osteoporosis. These microbes may affect activity of osteoclasts and osteoblasts to affect bone metabolism. We selected 5 health people and 10 osteoporosis patients and found that the diversity of gut microbiota in osteoporosis patients are decreased and imbalance with lower abundance of lactobacillus and butylic acid bacteria, meanwhile, 25-hydroxyitamin D and procollagen type I N-terminal peptide (PINP) of osteoporosis patient was significantly lower than the normal group. The proliferation, differentiation and maturity of osteoblasts MC3T3-E1 cells was stimulated, the activity of alkaline phosphatase, concentration of osteocalcin and the expression of RUNX2, WNT2 and CTNNB1 was improved by supernatant of lactobacillus acidophilus and butanoic acids, however, the proliferation, differentiation and maturity, the expression of RANK,WNT2 and CTNNB1 in osteoclasts RAW 264.7 cells was suppressed. There is not a similar significant effect by lactobacillus rhamnosus treatment.
Novel MprF amino acid mutations contributing to daptomycin non-susceptibility
Authors: Kawada Hayato , Hori Tsubasa , Nakamura Masaki , Kimura Yuko , Sato Kurumi , Yoshida Mayu , Matsui Hidehito , Hanaki Hideaki , Kitasato Hidero 
Published: September 2019

Abstract
Background
Daptomycin (DAP) is a cyclic lipopeptide antibiotic with potent bactericidal activity against gram-positive bacteria, and use to treat infections due to methicillin-resistant Staphylococcus aureus (MRSA). It was reported that MprF that is bacterial membrane protein and affect to DAP non-susceptible (DAP-NS), and it was generally considered that electrically repulsion by MprF mutation was thought to be contributed to DAP-NS. However, a lot of mechanisms were proposed previously and still controversial. Therefore, in this study, we found new MprF mutations from clinical MRSAs then introduced those MprF mutations into sequenced strain ( Staphylococcus aureus N315) in order to evaluate those effects to DAP-NS.
Results
We analysed the drug susceptibility profile of three MRSA clinical isolates and performed MprF sequence. Two of the three MRSA isolates showed DAP-NS and had different mutations of MprF (N450_I451 insI) and MprF (T345P), which have not been reported before. Then the mutations we identified were introduced into Staphylococcus aureus N315 (S. aureus N315), and we evaluated its susceptibility profile and positive charge of cell envelop to elucidate effect of DAP susceptbility. The MIC of DAP was increased by introduction of these mutations into S. aureus N315. In addition, the positive charge of the cell membrane was significantly increased in MprF(T345P), but not in MprF(N450_I451 insI).
Conclusions
These findings suggest that the two novel mutations contribute to DAP-NS. Furthermore, we suggested that the mechanism of DAP-NS was different depending on the mutation type and/or position of the amino acid.
Gestational diabetes mellitus is associated with antenatal hypercoagulability and secondary hyperfibrinolysis: a case control study of Chinese women
Authors: Liu Yan , Sun Xiaofang , Tao Junxian , Song Bin , Wu Wei , Li Ying , Sun Xing , Lu Dan , Zhu Danmo , Liu Chao , Cui Ji 
Published: September 2019

Abstract
Background : To determine the relationship between gestational diabetes mellitus (GDM) and coagulation/fibrinolysis disorders in antenatal Chinese women . Methods: Case control study. Fifty women had GDM and 132 did not (the NGDM group). Maternal plasma biochemistry and previous medical history were collected from perinatal health records. Antenatal coagulation/fibrinolysis were assessed using thromboelastography and traditional measures, then the relationship between coagulation/fibrinolysis and GDM was analyzed by multiple regression analysis. Results: GDM was significantly associated with higher activated partial thromboplastin time (odds ratio [OR] 1.5, 95% confidence interval [CI] 0.4–2.6); fibrinogen (OR 0.3, 95% CI 0.1–0.6); and percentage reduction in clot lysis after 30 min (OR 1.2, 95% CI 0.2–2.2), after adjustment for potential confounding factors. Both the intraoperative (238.2±71.0 ml vs . 286.0±102.4 ml, P =0.003) and 24-hour after surgery (270.7±99.8 ml vs . 314.7±131.1 ml, P =0.033) blood loss were lower and the prevalence of cesarean delivery (56.0% vs . 37.9%, P =0.027) was higher in the GDM group. There were no significant differences in the prevalence of maternal thrombotic events or maternal body mass before delivery. Conclusions: GDM is significantly associated with hypercoagulability and secondary hyperfibrinolysis in these antenatal Chinese women.