bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD93 hematopoietic stem cells improve diabetic wound healing by VEGF activation and downregulation of DAPK‐1
Authors: Zafari Fariba , Shirian Sadegh , Sadeghi Morteza , Teimourian Shahram , Bakhtiyari Mehrdad 
Published: September 2019

AbstractDiabetes is associated with numerous complications, such as diabetic skin wounds or ulcerations. The aim of this study was to evaluate experimentally the effectiveness of applying polycaprolactone (PCL)‐gelatin scaffold, with or without rat CD93 hematopoietic stem cells (HSCs), in diabetic wound healing in a rat model. CD93 HSCs were aseptically isolated from rat bone marrow using fluorescent activated cell sorting (FACS) method and FACS‐SORTER. A total of 25 Wistar rats were divided into five groups including Group I (sham, nondiabetic, and wound covered only with sterile dressing), II (control, diabetic rat), III (CD93 HSCs alone), IV (PCL‐gelatin scaffold), and V (CD93 HSCs+PCL‐gelatin scaffold). Animals were killed on Days 7, 14, or 28 posttreatment and histological sections were blindly evaluated by two expert pathologists. Death‐associated protein kinase 1 (DAPK‐1) gene and vesicular endothelial growth factors (VEGF) protein expression were evaluated using reverse transcription‐polymerase chain reaction and western blot, respectively. The thickest and the thinnest epidermises microscopically were belonged to CD93+HSCs+scaffold and the control group, respectively. The growth rate of the epidermis and adnexal epithelia was the highest in both the cell and cell+scaffold groups. Evaluation of the protein expression level of VEGF indicated that the expression levels of this growth factor were the most on Day 7 posttreatment in sham, HSCs alone, and HSCs cell+scaffold groups. While the lowest expression levels of this growth factor was detected in the control and scaffold groups. The gene expression level of DAPK‐1 on Day 7 posttreatment was higher than that of the Day 14 posttreatment in all groups. The highest and lowest gene expression levels of DAPK‐1 belonged to control and sham groups, respectively. According to our findings, CD93 HSCs offer new prospects for the treatment of diabetic ulcers and concomitant application of these cells with PCL‐gelatin nanofiber scaffold significantly improves diabetic wound treatment.
Differential expression of lncRNA/miRNA/mRNA and their related functional networks during the osteogenic/odontogenic differentiation of dental pulp stem cells
Authors: Liu Zhongjun , Xu Shuaimei , Dao Junfeng , Gan Zekun , Zeng Xiongqun 
Published: September 2019

AbstractDentin‐pulp regeneration requires dental pulp stem cells (DPSCs), but the role of long noncoding RNAs (lncRNAs) during this process remains unclear. Here, we cultured human DPSCs in osteogenic/odontogenic medium for 14 days and analyzed cells via RNA‐sequencing. The data were validated by quantitative reverse transcription‐polymerase chain reaction and lncRNA–microRNA (miRNA)–messenger RNA (mRNA) networks were constructed to reveal the potential competing endogenous RNA regulatory role of lncRNAs. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analysis were performed. One lncRNA, SNHG7, was identified and validated by genetic shRNA silencing. A total of 89 lncRNAs, 1,636 mRNAs, and 113 miRNAs were differentially expressed after differentiation. Bioinformatics identified an array of affected signaling pathways including phosphoinositide‐3‐kinase–protein kinase B, transforming growth factor‐β, and Wnt. mRNAs were enriched in cell migration, cell differentiation, stem cell development, ossification, and skeletal development. One lncRNA, SNHG7, was indentified to inhibit the odonto/osteogenic differentiation of DPSCs when silenced. In summary, we reveal several lncRNAs that significantly change during DPSC differentiation, including SNHG7. This reveals new targets for dentin‐pulp complex regeneration and tissue engineering.
Characterization of C2C12 cells in simulated microgravity: Possible use for myoblast regeneration
Authors: Calzia Daniela , Ottaggio Laura , Cora Alessandro , Chiappori Giorgia , Cuccarolo Paola , Cappelli Enrico , Izzotti Alberto , Tavella Sara , Degan Paolo 
Published: September 2019

AbstractMuscle loss is a major problem for many in lifetime. Muscle and bone degeneration has also been observed in individuals exposed to microgravity and in unloading conditions. C2C12 myoblst cells are able to form myotubes, and myofibers and these cells have been employed for muscle regeneration purposes and in myogenic regeneration and transplantation studies. We exposed C2C12 cells in an random position machine to simulate microgravity and study the energy and the biochemical challenges associated with this treatment. Simulated microgravity exposed C2C12 cells maintain positive proliferation indices and delay the differentiation process for several days. On the other hand this treatment significantly alters many of the biochemical and the metabolic characteristics of the cell cultures including calcium homeostasis. Recent data have shown that these perturbations are due to the inhibition of the ryanodine receptors on the membranes of intracellular calcium stores. We were able to reverse this perturbations treating cells with thapsigargin which prevents the segregation of intracellular calcium ions in the mitochondria and in the sarco/endoplasmic reticula. Calcium homeostasis appear a key target of microgravity exposure. In conclusion, in this study we reported some of the effects induced by the exposure of C2C12 cell cultures to simulated microgravity. The promising information obtained is of fundamental importance in the hope to employ this protocol in the field of regenerative medicine
The Durable, Dynamic Nature of Genre and Science: A Purpose‐Driven Typology of Science Trade Books
Authors: May Laura , Crisp Thomas , Bingham Gary E. , Schwartz Renée S. , Pickens Mario T. , Woodbridge Kate 
Published: September 2019

AbstractThe authors conducted a qualitative content analysis of the 400 National Science Teachers Association Outstanding Science Trade Books for Students K–12 from 2010–2017 selected for their instructional usefulness in science classrooms. The authors examined these expert‐recommended science trade books to better understand their design and to identify the various aspects of science education to which various genres may contribute. In light of previous research demonstrating the importance of texts used in science classrooms, the authors attended to the books’ scientific discourses and features. The authors present a typology helpful for understanding the books as related to science education broadly. The authors found general alignment between books that use expository language and those genres most likely to present knowledge accepted as reliable by the relevant scientific community. Genres apt to support the development of understandings about the nature of science and scientific inquiry, however, were more often composed of narrative. Given the richness of the text set and the multiple discourses therein, the authors were unable to bring their typology into alignment with arguments that certain kinds of trade books are unsuitable for science classrooms (e.g., biography, realistic fiction). A challenge with much of the existing research on science trade books and their use is that it has used oversimplified binary categories of narrative/expository to encourage limiting exposure to a single discourse or genre. Ultimately, through the typology presented, the authors seek to support the ability of science educators and researchers in identifying how different types of science trade books align with comprehensive science education.
A General and Straightforward Route to Noble Metal‐Decorated Mesoporous Transition‐Metal Oxides with Enhanced Gas Sensing Performance
Authors: Ma Junhao , Xiao Xingyu , Zou Yidong , Ren Yuan , Zhou Xinran , Yang Xuanyu , Cheng Xiaowei , Deng Yonghui 
Published: September 2019

AbstractControllable and efficient synthesis of noble metal/transition‐metal oxide (TMO) composites with tailored nanostructures and precise components is essential for their application. Herein, a general mercaptosilane‐assisted one‐pot coassembly approach is developed to synthesize ordered mesoporous TMOs with agglomerated‐free noble metal nanoparticles, including Au/WO3, Au/TiO2, Au/NbOx, and Pt/WO3. 3‐mercaptopropyl trimethoxysilane is applied as a bridge agent to cohydrolyze with metal oxide precursors by alkoxysilane moieties and interact with the noble metal source (e.g., HAuCl4 and H2PtCl4) by mercapto (SH) groups, resulting in coassembly with poly(ethylene oxide)‐b‐polystyrene. The noble metal decorated TMO materials exhibit highly ordered mesoporous structure, large pore size (≈14–20 nm), high specific surface area (61–138 m2 g−1), and highly dispersed noble metal (e.g., Au and Pt) nanoparticles. In the system of Au/WO3, in situ generated SiO2 incorporation not only enhances their thermal stability but also induces the formation of ε‐phase WO3 promoting gas sensing performance. Owning to its specific compositions and structure, the gas sensor based on Au/WO3 materials possess enhanced ethanol sensing performance with a good response (Rair/Rgas = 36–50 ppm of ethanol), high selectivity, and excellent low‐concentration detection capability (down to 50 ppb) at low working temperature (200 °C).
The effect of tree age on the chemical composition of the wood of silver birch (Betula pendula Roth.) in Poland
Authors: Lachowicz Hubert , Wróblewska Hanna , Wojtan Rafał , Sajdak Magdalena 
Published: September 2019

Abstract
The article presents the results of a so far most extensive study on the effect of tree age on variation of the chemical structure of silver birch wood carried out in Poland. Field studies took place in 12 forestry districts distributed throughout the country. In each district, study sites were selected with stands aged approximately 30, 50, and 70 years in fresh broadleaved forest habitats, as well as in five districts in fresh mixed broadleaved forest habitats. Analyses were made of the contents of substances soluble in cold and hot water, ethanol and 1% NaOH, as well as the contents of cellulose, lignin, pentosans and ash, and the pH, of wood from 306 silver birch trees. Tree age was shown to have a significant effect on the contents of cellulose, pentosans, ash, and substances soluble in 1% NaOH. In the case of the other characteristics, age was found to have no significant effect. The cellulose content was lowest in 30-year-old stands and increased with tree age. Similar observations were made regarding ash content. Contents of pentosans and substances soluble in 1% NaOH were highest in the wood of the youngest trees. Since birch wood is used by the pulp and paper, board and energy industries, the knowledge of its chemical composition, depending on the age of the trees, can be of potential practical importance for its further application.
Tumor size  ≥50 mm as an Independent Prognostic Factor for Patients with Stage II or III Gastric Cancer After Postoperative S‐1 Monotherapy: Analysis of a Multi‐institution Dataset
Authors: Tsutsuyama Masayuki , Kanda Mitsuro , Ito Seiji , Mochizuki Yoshinari , Teramoto Hitoshi , Ishigure Kiyoshi , Murai Toshifumi , Asada Takahiro , Ishiyama Akiharu , Matsushita Hidenobu , Tanaka Chie , Kobayashi Daisuke , Fujiwara Michitaka , Murotani Kenta , Kodera Yasuhiro 
Published: September 2019

AbstractBackgroundLittle is known about the changes in prognostic factors after adjuvant S‐1 monotherapy has become widespread as a standard of care for patients with gastric cancer (GC) in East Asia. The present study compared prognostic factors of patients with stage II/III GC treated with or without S‐1 adjuvant to formulate appropriate risk stratification strategies.MethodsWe designed a large multicenter dataset and retrospectively analyzed 847 patients with GC stage II or III, who underwent curative gastrectomy between 2010 and 2014. Clinicopathological features and prognostic factors were compared between the two patient groups: surgery‐alone (n = 266) and S‐1 adjuvant (n = 581).ResultsThere were no significant differences in pathological tumor depths, nodal status, and disease stages between groups. Recurrence‐free survival was significantly longer in the S‐1 adjuvant group. For the surgery‐alone group, independent prognostic factors were (in order of hazard ratio): (1) invasive growth, (2) high preoperative carcinoembryonic antigen levels, (3) total gastrectomy. For the S‐1 adjuvant group, macroscopic tumor size (≥50 mm) was identified as another independent prognostic factor next only to pN2/3. There was overlap between the survival curves of patients with tumor size  ≥50 mm in both groups. After receiving adjuvant S‐1 monotherapy,  ≥50 mm patients had significantly higher prevalence of peritoneal and lymph node metastasis as initial recurrences compared with  <50 mm patients.ConclusionsAdjuvant S‐1 monotherapy may alter listing of adverse prognostic factors of stage II and III patients. Macroscopic tumor size  ≥50 mm may serve as an important determinant for risk stratification to identify patients who require more intensive treatment.
Hematocrit levels and thrombotic events in patients with polycythemia vera: an analysis of Veterans Health Administration data
Authors: Parasuraman Shreekant , Yu Jingbo , Paranagama Dilan , Shrestha Sulena , Wang Li , Baser Onur , Scherber Robyn 
Published: September 2019

Abstract
Patients with polycythemia vera (PV) have a high incidence of thrombotic events (TEs), contributing to a greater mortality risk than the general population. The relationship between hematocrit (HCT) levels and TE occurrence among patients with PV from the Veterans Health Administration (VHA) was evaluated to replicate findings of the CYTO-PV trial with a real-world patient population. This retrospective study used VHA medical record and claims data from the first claim with a PV diagnosis (index) until death, disenrollment, or end of study, collected between October 1, 2005, and September 30, 2012. Patients were aged ≥ 18 years at index, had ≥ 2 claims for PV (ICD-9-CM code, 238.4) ≥ 30 days apart during the identification period, continuous health plan enrollment from 12 months pre-index until end of study, and ≥ 3 HCT measurements per year during follow-up. This analysis focused on patients with no pre-index TE, and with all HCT values either < 45% or ≥ 45% during the follow-up period. The difference in TE risk between HCT groups was assessed using unadjusted Cox regression models based on time to first TE. Patients (N = 213) were mean (SD) age 68.9 (11.5) years, 98.6% male, and 61.5% white. TE rates for patients with HCT values < 45% versus ≥ 45% were 40.3% and 54.2%, respectively. Among patients with ≥ 1 HCT before TE, TE risk hazard ratio was 1.61 (95% CI, 1.03–2.51; P = 0.036). This analysis of the VHA population further supports effective monitoring and control of HCT levels < 45% to reduce TE risk in patients with PV.
Relationships between recovery experiences and well-being among younger and older teachers
Authors: Virtanen Anniina , De Bloom Jessica , Kinnunen Ulla 
Published: September 2019

AbstractPurposeThe study had three aims. We investigated, first, how six recovery experiences (i.e., detachment, relaxation, control, mastery, meaning, and affiliation) during off-job time suggested by the DRAMMA model (Newman et al. in J Happiness Stud 15(3):555–578.10.1007/s10902-013-9435-x, 2014) are related to well-being (i.e., vitality, life satisfaction, and work ability). Second, we examined how age related to these outcomes, and third, we investigated whether age moderated the relationships between recovery experiences and well-being outcomes.MethodsA sample of 909 Finnish teachers responded to an electronic questionnaire (78% women, average age 51 years). The data were analyzed with moderated hierarchical regression analyses.ResultsDetachment from work, relaxation, control, and mastery were associated with higher vitality. Detachment, relaxation, meaning, and affiliation were related to higher life satisfaction. Older age was related to lower work ability, but not to vitality or life satisfaction. Older teachers benefited more from control and mastery during off-job time than did younger teachers in terms of vitality, whereas younger teachers benefited more from relaxation in terms of all well-being outcomes.ConclusionsDetachment, relaxation, control, mastery, meaning, and affiliation during off-job time were related to higher well-being, supporting the DRAMMA model. Age moderated the relationships between control, mastery, and relaxation and vitality and life satisfaction. The role of aging in recovery from work needs further research.
Factors related to parental pre-treatment motivation in outpatient child and adolescent mental health care
Authors: Drent Halewijn M. , van den Hoofdakker Barbara , de Bildt Annelies , Buitelaar Jan K. , Hoekstra Pieter J. , Dietrich Andrea 
Published: September 2019

AbstractThe aim of this study was to investigate the relation between a variety of child, parent, family and environmental factors and pre-treatment motivation of parents of children and adolescents newly referred to a mental health care clinic in The Netherlands. Data were collected of 521 parents most involved in the upbringing of the child (443 mothers and 78 fathers; Dutch origin 97.1%) of 207 girls and 314 boys (ageM = 10.2, range 1–18 years). Treatment motivation was measured by the Parent Motivation Inventory. Least absolute shrinkage and selection operator (LASSO) regression was used to investigate the prediction strength of 33 factors in 5 domains: (1) source of referral and prior use of healthcare services, (2) child characteristics, (3) characteristics of the primary parent, (4) parenting characteristics of the primary parent, and (5) family characteristics. Twenty-one factors were statistically relevant, explaining 21.3% of the deviance in pre-treatment motivation. Child characteristics, mainly type and severity of problems, contributed most to the model. Notably, internalising problems contributed more than externalising problems. Furthermore, we found relations between parental pre-treatment motivation and parents' perceived self-efficacy, parents' perceived parenting competence, financial problems and source of referral. Our findings provide insight into the multifacetedness of parental motivation prior to starting treatment and inform health professionals of specific contextual factors of interest in parents' readiness to change their behaviour and participate in treatment.
Using the lead crack framework to reduce durability test duration
Authors: Molent L. , Singh R. 
Published: September 2019

ABSTRACTAircraft full-scale fatigue tests are expensive and time-consuming to conduct but are a critical item on the certification path of any aircraft design or modification. This paper outlines a proposal that trades cycling hours for increased detail in the teardown of a metallic test article. A method for determining the equivalent demonstrated crack size (and crack growth curve) at the mandated test life utilising the lead crack framework is demonstrated. It is considered that the test duration can be significantly reduced, whilst still achieving all the desired outcomes of a certification program.
UVB-induced DHODH upregulation, which is driven by STAT3, is a promising target for chemoprevention and combination therapy of photocarcinogenesis
Authors: Hosseini Mohsen , Dousset Léa , Michon Pauline , Mahfouf Walid , Muzotte Elodie , Bergeron Vanessa , Bortolotto Doriane , Rossignol Rodrigue , Moisan François , Taieb Alain , Bouzier-Sore Anne-Karine , Rezvani Hamid R. 
Published: September 2019

AbstractThe leading cause of cutaneous squamous cell carcinomas (cSCCs) is exposure to ultraviolet radiation (UV). Unlike most other cancers, the incidence rates of cSCCs are still on the rise and the treatment options currently available are limited. We have recently found that dihydroorotate dehydrogenase (DHODH), which is the rate-limiting enzyme in the de novo pyrimidine synthesis pathway, plays a critical role in UVB-induced energy metabolism reprogramming. Using a multistage model of UVB radiation-induced skin cancer, we show that UVB-induced DHODH upregulation is mainly regulated transcriptionally by STAT3. Our results indicate that chronic inhibition of DHODH by leflunomide (LFN) blocks UVB-induced tumor initiation. Human tumor xenograft studies showed that LFN treatment reduces growth of established tumors when used in combination with a genotoxic agent, 5-fluorouracil (5-FU). Our data suggest that DHODH is a promising target for chemoprevention and combination therapy of UVB-induced cSCCs.
Skeletal adaptations in young male mice after 4 weeks aboard the International Space Station
Authors: Maupin Kevin A. , Childress Paul , Brinker Alexander , Khan Faisal , Abeysekera Irushi , Aguilar Izath Nizeet , Olivos David J. , Adam Gremah , Savaglio Michael K. , Ganesh Venkateswaran , Gorden Riley , Mannfeld Rachel , Beckner Elliott , Horan Daniel J. , Robling Alexander G. , Chakraborty Nabarun , Gautam Aarti , Hammamieh Rasha , Kacena Melissa A. 
Published: September 2019

AbstractGravity has an important role in both the development and maintenance of bone mass. This is most evident in the rapid and intense bone loss observed in both humans and animals exposed to extended periods of microgravity in spaceflight. Here, cohabitating 9-week-old male C57BL/6 mice resided in spaceflight for ~4 weeks. A skeletal survey of these mice was compared to both habitat matched ground controls to determine the effects of microgravity and baseline samples in order to determine the effects of skeletal maturation on the resulting phenotype. We hypothesized that weight-bearing bones would experience an accelerated loss of bone mass compared to non-weight-bearing bones, and that spaceflight would also inhibit skeletal maturation in male mice. As expected, spaceflight had major negative effects on trabecular bone mass of the following weight-bearing bones: femur, tibia, and vertebrae. Interestingly, as opposed to the bone loss traditionally characterized for most weight-bearing skeletal compartments, the effects of spaceflight on the ribs and sternum resembled a failure to accumulate bone mass. Our study further adds to the insight that gravity has site-specific influences on the skeleton.
Gaia 8: Discovery of a star cluster containing β Lyrae
Authors: Bastian U. 
Published: September 2019

Aims. The aim here is to try and clarify whether there are stars in the neighbourhood of β Lyrae which are physically connected to the famous prototype eclipsing binary.
Methods. We scrutinised the astrometric and photometric data (from HIPPARCOS and Gaia DR2) of stars in a limited sky field around β Lyrae.
Results. A star cluster of about 100 members was discovered, nicely centred on β Lyrae in five dimensions. There are clear indications that the newly discovered cluster belongs to a larger old (extinct) star formation complex, including two known star clusters and an extended, strewn field of stars at practically the same distance and space velocity.
Conclusions. This discovery opens up the possibility of determining a precise distance and precise age for β Lyrae using the combined properties of the cluster members. This in turn may form an important basis for an improved astrophysical interpretation of the model binary β Lyrae and its kin. The larger old star formation complex is of scientific interest in its own right.