bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early Mortality in Children and Adolescents with Acute Promyelocytic Leukemia: Experience of the Boldrini Children’s Center
Authors: de Azevedo Amilcar C. , Matsuda Eduardo , Cervellini Julia Y. , Prandi Larissa R. , Omae Cristiane , Jotta Patricia Y. , Pereira Ricardo M. , Brandalise Silvia R. 
Published: October 2019

Introduction:
Acute promyelocytic leukemia (APL) is currently considered a highly curable disease. However, an early death (ED) remains one of the main causes of APL treatment failure.

Patients and Methods:
In this retrospective study, we aimed to analyze the clinical characteristics of 91 children and adolescents with APL, who were consecutively registered at the (name of institution removed) Children’s Center from January 1, 1998 to December 31, 2017. Data were assessed for age, sex, ethnicity, body mass index percentile, initial white blood cell count, peripheral blood blast count, and platelet count, hemoglobin value, partial thromboplastin time, prothrombin time, fibrinogen level, serum creatinine level, APL morphology subtype (classic vs. hypogranular variant M3v), and FLT3 gene mutations.

Results:
ED occurred in 12 of 91 (13.1%) patients and was mainly related to cerebral thromboembolism. Overall 66% of deaths occurred in the second week after diagnosis. ED was associated with white blood cell ≥10×109 cells/L (odds ratio of 8.44; 95% confidence interval [CI]=1.48-48.26; P=0.0016), initial promyelocytes ≥20×109/L (odds ratio of 9.29; 95% CI=2.45-35.8; P=0.001), morphologic subtype M3v (odds ratio of 3.63; 95% CI=1.04-12.64; P=0.043), and creatinine serum levels >0.7 mg/dL (odds ratio of 6.78; 95% CI=1.83-25.13; P=0.004). In multivariate analyses, ED was associated with initial peripheral promyelocytes ≥20×109 blasts/L and creatinine serum levels >0.7 mg/dL.

Conclusions:
EDs were mainly caused by thrombohemorrhagic events and occurred within the second week after diagnosis. High peripheral promyelocytes and creatinine levels were predictors of ED in APL.
Comparison of tuning properties of gamma and high-gamma power in local field potential (LFP) versus electrocorticogram (ECoG) in visual cortex
Authors: Dubey Agrita , Ray Supratim 
Published: October 2019

AbstractElectrocorticogram (ECoG), obtained from macroelectrodes placed on the cortex, is typically used in drug-resistant epilepsy patients, and is increasingly being used to study cognition in humans. These studies often use power in gamma (30-70 Hz) or high-gamma (>80 Hz) ranges to make inferences about neural processing. However, while the stimulus tuning properties of gamma/high-gamma power have been well characterized in local field potential (LFP; obtained from microelectrodes), analogous characterization has not been done for ECoG. Using a hybrid array containing both micro and ECoG electrodes implanted in the primary visual cortex of two female macaques, we compared the stimulus tuning preferences of gamma/high-gamma power in LFP versus ECoG and found them to be surprisingly similar. High-gamma power, thought to index the average firing rate around the electrode, was highest for the smallest stimulus (0.3° radius), and decreased with increasing size in both LFP and ECoG, suggesting local origins of both signals. Further, gamma oscillations were similarly tuned in LFP and ECoG to stimulus orientation, contrast and spatial frequency. This tuning was significantly weaker in electroencephalogram (EEG), suggesting that ECoG is more like LFP than EEG. Overall, our results validate the use of ECoG in clinical and basic cognitive research.
DeepCINAC: a deep-learning-based Python toolbox for inferring calcium imaging neuronal activity based on movie visualization
Authors: Denis Julien , Dard Robin F. , Quiroli Eleonora , Cossart Rosa , Picardo Michel A. 
Published: October 2019

AbstractTwo-photon calcium imaging is now widely used to infer neuronal dynamics from changes in fluorescence of an indicator. However, state of the art computational tools are not optimized for the reliable detection of fluorescence transients from highly synchronous neurons located in densely packed regions such as the CA1 pyramidal layer of the hippocampus during early postnatal stages of development. Indeed, the latest analytical tools often lack proper benchmark measurements. To meet this challenge, we first developed a graphical user interface allowing for a precise manual detection of all calcium transients from imaged neurons based on the visualization of the calcium imaging movie. Then, we analyzed the movies using a convolutional neural network with an attention process and a bidirectional long-short term memory network. This method is able to reach human performance and offers a better F1 score (harmonic mean of sensitivity and precision) than CaImAn to infer neural activity in the developing CA1 without any user intervention. It also enables automatically identifying activity originating from GABAergic neurons. Overall, DeepCINAC offers a simple, fast and flexible open-source toolbox for processing a wide variety of calcium imaging datasets while providing the tools to evaluate its performance.Significance statementInferring neuronal activity from calcium imaging data remains a challenge due to the difficulty in obtaining a ground truth using patch clamp recordings and the problem of finding optimal tuning parameters of inference algorithms. DeepCINAC offers a flexible, fast and easy-to-use toolbox to infer neuronal activity from any kind of calcium imaging dataset through visual inspection.
Single-cell multiomics sequencing reveals the functional regulatory landscape of early embryos
Authors: Wang Yang , Yuan Peng , Yan Zhiqiang , Yang Ming , Huo Ying , Nie Yanli , Zhu Xiaohui , Yan Liying , Qiao Jie 
Published: October 2019

AbstractExtensive epigenetic reprogramming occurs during preimplantation embryo development and is accompanied by zygotic genome activation (ZGA) and first cell fate specification. Recent studies using single-cell epigenome sequencing techniques have provided global views of the dynamics of different epigenetic layers during this period. However, it remains largely unclear how the drastic epigenetic reprogramming contributes to transcriptional regulatory network. Here, we developed a single-cell multiomics sequencing technology (scNOMeRe-seq) that enables profiling of genome-wide chromatin accessibility, DNA methylation and RNA expression in the same individual cell with improved performance compared to that of earlier techniques. We applied this method to analyze the global dynamics of different molecular layers and their associations in mouse preimplantation embryos. We found that global DNA methylation remodeling facilitates the reconstruction of genetic lineages in early embryos and revealed that the gradual increases in heterogeneity among blastomeres are driven by asymmetric cleavage. Allele-specific DNA methylation pattern is maintained throughout preimplantation development and is accompanied by allele-specific associations between DNA methylation and gene expression in the gene body that are inherited from oocytes and sperm. Through integrated analyses of the collective dynamics between gene expression and chromatin accessibility, we constructed a ZGA-associated regulatory network and revealed coordination among multiple epigenetic layers, transcription factors (TFs) and repeat elements that instruct the proper ZGA process. Moreover, we found that inner cell mass (ICM)/trophectoderm (TE) lineage-associated cis-regulatory elements are stepwise activated in blastomeres during post-ZGA embryo stages. TE lineage-specific TFs play dual roles in promoting the TE program while repressing the ICM program, thereby separating the TE lineage from the ICM lineage. Taken together, our findings not only depict the first single-cell triple-omics map of chromatin accessibility, DNA methylation and RNA expression during mouse preimplantation development but also enhance the fundamental understanding of epigenetic regulation in early embryos.
“Viscotaxis”- Directed Migration of Mesenchymal Stem Cells in Response to Loss Modulus Gradient
Authors: Shirke Pallavi Uday , Goswami Hiya , Kumar Vardhman , Shah Darshan , Das Siddhartha , Bellare Jayesh , Venkatesh K.V. , Seth Jyoti R. , Majumder Abhijit 
Published: October 2019

AbstractDirected cell migration in response to chemical and mechanical gradients plays a crucial role in physiological and pathological conditions. One such mechanical cues that is known to influences cell migration is the gradient of substrate elastic modulus (E). However, the elastic modulus alone cannot fully define the material properties of the cellular microenvironment, which often has both elastic and viscous characteristics. In this study, we investigated the influence of the gradient of viscous nature, as defined by loss modulus, G”, on cell migration. We cultured human mesenchymal stem cells (hMSCs) on a collagen-coated polyacrylamide gel with constant elastic property, as defined by the storage modulus G’, but with the gradient of loss modulus G”. We found hMSCs to migrate from high to low loss modulus. We have termed this, thus far unreported, directional cellular migration as “Viscotaxis”. We have confirmed uniform collagen density and constant storage modulus of the gel by fluorescence microscopy and atomic force microscopy to eliminate the possibilities of haptotaxis and durotaxis. We hypothesize that material creep in the high loss modulus region hinders the building up of the cellular traction, leading to a force asymmetry that drives the observed viscotaxis. To verify our hypothesis, we estimated the cellular traction on gels with high and low loss moduli. We indeed found that cells apply higher traction force on more elastic materials i.e. materials with low loss modulus. On the disruption of actomyosin contractility with myosin inhibitor blebbistatin and ROCK inhibitor Y27632, directional migration was lost. Further, we showed that cells can maintain a stable morphology on a low loss modulus substrate but due to its inability to build up stable cellular traction on a substrate with high loss modulus, the cell spreading remains in a dynamic state. Our findings in this paper highlight the importance of considering the viscous modulus while preparing stiffness-based substrates for the field of tissue engineering.
Deteriogenic flora of the Phlegraean Fields Archaeological Park: ecological analysis and management guidelines
Authors: Motti Riccardo , Bonanomi Giuliano , Stinca Adriano 
Published: October 2019

AbstractBiodeterioration, the alteration caused by living organisms, on historical buildings and stone monuments is a well-known problem affecting two-thirds of the world’s cultural heritage. The study of the flora growing on wall surface is of particular importance for the assessment of the risk of biodeterioration of stone artifacts by vascular plants, and for maintenance planning. In this study, we investigate how rock type, exposure and inclination of the wall affect the biodeteriogenic flora at 13 sites of the Archaeological Park of the Phlegraean Fields located in the province of Naples, in southern Italy. For each site, we analysed randomly selected square areas with 2 × 2 m size, representing the different vegetation types in terms of vascular plant species cover. The total number of plant species recorded was 129, belonging to 43 families.Erigeron sumatrensis, Sonchus tenerrimus, andParietaria judaicaare the most commonly reported species, whileCapparis orientalisis the species with the highest average coverage. Substrate type, exposure and surface inclination affect the floristic composition, with the average plant cover significantly higher on vertical surfaces and at western and southern exposure. All the main biodeteriogenic vascular plant species grow on more or less porous lythotype like yellow tufa, conglomerate and bricks. Finally, woody plants eradications methods are proposed by the tree cutting and local application of herbicides, to avoid stump and root sprouting and to minimize the dispersion of chemicals in the surrounding environment.
Municipal-owned community bank: capital raising dilemma
Authors: Swai Tobias Aloisi 
Published: October 2019

Learning outcomes
The case introduces student to basic understanding of banking sector in Tanzania as well as the strategies and struggle to raise capital through shareholders’ funds. Application of Banking theory and Pecking order theory is evidenced from the case. The case outlines why the bank struggled to raise capital and what triggers the capital raising strategies. It also give students an opportunity to think about applicable theories of capital structure and bank capital, and strategies the bank could use to rescue its capital crunch in the future.

Case overview/synopsis
The case provides details of how the Capital Community Bank (CCB) raised its capital through strategic financial engineering which enabled it to raise the minimum regulatory capital required to be licensed as a financial institution unit, to a regional financial institution, to a fully fledged commercial bank. The bank started with a paid up capital of TZS 472.3m in 2002, involving four Local Government Authorities and individual investors. Capital raised to TZS 31.3bn in 2014 and down to TZS 20.6bn at the end of 2016. The minimum regulatory capital required is TZS 15bn, while paid up capital was 16.9bn. With the change of the management team in 2017, the bank is looking for avenues to raise further capital to meet the regulatory limits and continue to survive as a commercial bank, given dramatic changes in the banking sector in Tanzania.

Complexity academic level
The case is suitable for third year students in Bachelor of Commerce/Economics specializing in banking/financial services. It also suits postgraduate/master's students seeking a Postgraduate Diploma or Master of Business Administration in financial institutions/banking course.

Supplementary materials
Teaching Notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes.

Subject code
CSS 1: Accounting and Finance.
Not such an ordinary life: a comparison of employment, marital status and housing profiles of adults with and without intellectual disabilities
Authors: McMahon Martin , Bowring Darren Lee , Hatton Chris 
Published: October 2019

Purpose
Having paid work, relationships and a choice of where to live are common policy priorities for adults with intellectual disabilities. The purpose of this paper is to compare outcomes with respect to these three priorities between adults with intellectual disability and the general population in Jersey.

Design/methodology/approach
Data were collected from 217 adults with intellectual disability known to services, and 2,350 adults without intellectual disability using a stratified random sample. Data on employment, marital status and accommodation profiles were compared.

Findings
In sum, 87 per cent of adults with intellectual disability were currently single vs 16 per cent of adults without intellectual disability; 23 per cent of working-age adults with intellectual disability were in paid employment vs 92 per cent of working-age adults without intellectual disability; and 57 per cent of adults with intellectual disability lived-in sheltered housing vs 2 per cent of adults without intellectual disability.

Social implications
Very few adults with intellectual disability are in paid employment or intimate relationships, and the majority live in sheltered, supported housing, with very few owning their own home. There is a significant disconnect between policy and reality. Considerable work is required to make an ordinary life the reality for adults with intellectual disability.

Originality/value
This study adds to the body of evidence that suggests people with intellectual disabilities are less likely to experience an ordinary life. Furthermore, it illustrates that despite Jersey being an affluent society, the same difficulties and barriers exist there for persons with an intellectual disability as in other jurisdictions.
Could excision repair cross‐complementing group‐1 mRNA expression from peripheral blood lymphocytes predict locoregional failure with cisplatin chemoradiation for locally advanced laryngeal cancer?
Authors: Raturi Vijay Parshuram , Wu Chen‐Ta , Mohammad Suhel , Hojo Hidehiro , Bei Yanping , Nakamura Masaki , Okumura Masayuki , Rachi Toshiya , Singh Rahul , Gupta Rajeev , Parmar Devendra , Hasan Feza , Gaur Jalaj , Kishan Dewesh , Kumar Saurabh , Badajena Avinash , Katepogu Pranay , Shigematsu Naoyuki 
Published: October 2019

AbstractAimThe association of excision repair cross‐complementing 1 mRNA (ERCC‐1 mRNA) expression with the outcome has been reported with immunohistochemistry (IHC) using tumor tissue in head and neck cancer. We evaluated ERCC‐1 mRNA expression by reverse transcription polymerase chain reaction (RT‐PCR) from peripheral blood lymphocytes (PBLs) as bio‐predictor of locoregional failure (LRF) to chemoradiation (CRT) for locally advanced laryngeal squamous cell cancer (LALSCC).MethodsA total of 107 male patients with LALSCC were enrolled in this prospective study. ERCC‐1 mRNA expression by PBLs was determined by RT‐PCR. Definitive CRT was delivered with 35 mg/m2 weekly cisplatin. Response Evaluation Criteria in Solid Tumor 1.1 (RECIST 1.1) were used in evaluating treatment response. The primary objective was to assess LRF. The influence of patient characteristics, treatment response, weekly cisplatin cycles, ERCC mRNA expression was determined for LRF, progression‐free survival (PFS) and overall survival (OS).ResultsA total of 98 patients completed definitive CRT. The median value of 2‐ΔΔCT ERCC‐1 mRNA expression was 3.9; based on which it was categorized as low and high. Correlation of ERCC‐1 expression with treatment response was insignificant (P‐ .38). With a median follow‐up of 33 months; 2‐year LRF, PFS, and OS was 63.3%, 34.7% and 79.4%. The 2‐year LRF, PFS and OS for low versus high expression were 53.1% versus 73.5% (P‐value = 0.036), 44.9% versus 24.4% (P‐value = 0.047) and 81.6% versus 77.2% (P‐value = 0.33), respectively. In multivariate analysis, ERCC‐1 expression, T‐stage, N‐stage and tumor subsite are predictive factors for LRF; T‐stage and nodal recurrence for OS; stage and treatment response for PFS.ConclusionLALSCC patient with ERCC‐1 mRNA low expression was associated with lower LRF rate, and improved PFS.
The Political Importance of Financial Performance
Authors: Pond Amy , Zafeiridou Christina 
Published: October 2019

AbstractAsset mobility is thought to constrain taxation, as firms with mobile assets can avoid taxation by locating their assets in low‐tax jurisdictions. Firms with immobile assets then face higher taxes. By considering the political incentives that accompany widespread financialization, we identify a new limit to the targeting of immobile firms: Publicly traded firms with immobile underlying assets lose more value in financial markets when taxes are increased, as shareholders anticipate that these underlying assets cannot be withheld from taxation. When governments care about this loss in value, their incentive to tax immobile, publicly traded firms declines. Political concern for financial performance therefore limits the extent to which immobile assets can be targeted for taxation. We argue that broad‐based participation in the stock market and democratic political institutions increase political concern for financial performance. We discuss the implications of the theory and findings for policy autonomy, firm ownership, and economic voting.
Apical aortic blood pump preclinical assessment for long‐term use: Durability test and stator topology to reduce wear in the bearing system
Authors: da Silva Bruno Utiyama , da Fonseca Jeison Willian Gomes , Leal Edir Branzoni , Cardoso José Roberto , Biscegli José Francisco , de Andrade Aron José Pazin 
Published: October 2019

AbstractThis study presents an assessment for long‐term use of the apical aortic blood pump (AABP), focusing on wear reduction in the bearing system. AABP is a centrifugal left ventricle assist device initially developed for bridge to transplant application. To analyze AABP performance in long‐term applications, a durability test was performed. This test indicated that wear in the lower bearing pivot causes device failure in long‐term. A wear test in the bearing system was conducted to demonstrate the correlation of the load in the bearing system with wear. Results from the wear test showed a direct correlation between load and wear at the lower bearing pivot. In order to reduce load, thus reducing wear, a new stator topology has been proposed. In this topology, a radial stator would replace the axial stator previously used. Another durability test with the new stator has accounted twice the time without failure when compared with the original model.
The Relation Between Dialect Density and the Codevelopment of Writing and Reading in African American Children
Authors: Puranik Cynthia , Branum‐Martin Lee , Washington Julie A. 
Published: October 2019

The purpose of this longitudinal study was to examine the influence of spoken dialect density on writing and on the codevelopment of reading and writing in African American English‐speaking (AAE) children from first through fifth grades. The sample included 869 students, ranging in age from 5.8 to 12.5 years. Results indicated that dialect density had a negative influence concurrently and longitudinally on reading and writing in AAE‐speaking children. High dialect users tended to have weak reading and writing skills and heavier dialect density slowed growth in reading and writing. However, this effect was moderated by the effects of reading and writing on each other. Reading had a facilitative effect on writing even in the presence of heavy dialect use.
A systematic expert‐based assessment of progress and priorities for conservation of the Bengal Florican Houbaropsis bengalensis
Authors: Mahood Simon P. , Jha Rohit R. S. , Rahmani Asad R. , Thakuri Jyotendra J. , Chamnan Hong , Bhargava Rajat , Lahkar Bibhuti P. , Brahma Namita , Narwade Sujit S. , Pandey Naveen , Gore Kedar , Gadhvi Devesh , Virak Son , Garnett Stephen T. 
Published: October 2019

AbstractWe used information derived from expert elicitation to measure progress in conservation of the Bengal Florican (Houbaropsis bengalensis) by calculating six metrics, namely: (a) current threat impact, (b) research need, (c) research achievement, (d) management need, (e) management achievement, and (f) percentage threat reduction. We then used the metrics to identify priority threats, namely predation by dogs, hunting, power lines, and vegetation changes caused by human activities. The metrics indicate that although there has been little progress in addressing predation and power lines, reductions in hunting have been achieved and practitioners have a good understanding of how to ameliorate vegetation changes. We assessed conservation needs across all sites that support Bengal Florican, and highlight conservation achievements at Stoung‐Chikreang Bengal Florican Conservation Area (BFCA), Manas National Park and Kaziranga National Park. We used our results to develop a framework for prioritizing collaboration on tackling priority threats. We highlight D'Ering Wildlife Sanctuary in India as one of the highest priority sites for Bengal Florican conservation, and propose that conservation in Stoung‐Chikreang Bengal Florican Conservation Area (BFCA), Manas National Park and Kaziranga National Park must be continued, scaled‐up and good‐practice replicated at Koklabari, Baray BFCA and Koshi Tappu Wildlife Reserve. If these plans are followed, then we believe that priority threats can be adequately addressed and the extinction of Bengal Florican can be prevented.