bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluoroscopy as Definitive Postreduction Imaging of Pediatric Wrist and Forearm Fractures Is Safe and Saves Time
Authors: Goodman Avi D. , Walsh Devin F. , Zonfrillo Mark R. , Eberson Craig P. , Cruz Aristides I. 
Published: April 2019

Background:
Recent studies indicate that formal postreduction radiographs may be unnecessary for closed, isolated pediatric wrist, and forearm when mini C-arm fluoroscopy is used for reduction. Our institution changed the Emergency Department (ED) management protocol to reflect this by allowing orthopaedic providers to determine if fluoroscopy was acceptable to assess fracture reduction. We hypothesized that using fluoroscopy as definitive postreduction imaging would decrease total encounter time, without an increase in the rate of rereduction or surgery.

Methods:
Patients with closed, isolated distal radius/distal ulna (DR/DU) or both bone forearm (BBFA) fractures that required sedation and reduction under mini C-arm fluoroscopy at our Level 1 pediatric ED were reviewed for 6 months both before and after this policy change. Before, all patients had formal postreduction radiographs; after, the decision was left to the orthopaedic physician. Timestamp data were collected, as was the need for rereduction or surgery. In addition to descriptive statistics, between-group differences were analyzed with the Student t test, χ2 test, and multivariable regression as appropriate.

Results:
A total of 243 patients (119 before, 124 after) had 165 DR/DU and 78 BBFA fractures. Demographic data were similar before and after. After protocol implementation, univariable analysis (Student t test) showed that sedation times were longer, while total ED time and the time from sedation beginning to discharge were similar. The proportion of patients requiring rereduction or surgery were similar.
After multivariable regression, “fluoroscopy as definitive imaging” was the only independent determinant of the time intervals compared with using conventional radiography. Sedation was an average of 13.8 minutes longer (P<0.001), while the interval from sedation beginning to discharge was 15.8 minutes shorter (P=0.007), and total ED time was 33.0 minutes shorter (P=0.018). Fluoroscopy as definitive imaging was not a predictor of surgery (odds ratio=0.63, P=0.520), although having a BBFA increased the likelihood (odds ratio=4.50, P=0.008).

Conclusions:
Implementing a protocol in which the provider could use mini C-arm fluoroscopy for definitive postreduction imaging did not result in increased rates remanipulation or need for surgery. Regression analysis further demonstrated time savings associated with foregoing conventional radiographs.

Level of Evidence:
Level III—therapeutic.
Prevalence of Clostridium difficile infection among hospitalized inflammatory bowel disease patients in Greece
Authors: Viazis Nikos , Pontas Christos , Karmiris Konstantinos , Dimas Ioannis , Fragaki Maria , Paspatis Gregorios , Drygiannakis Ioannis , Koutroubakis Ioannis E. , Moschovis Dimitris , Tzouvala Maria , Theocharis Georgios , Tsolias Chrysostomos , Thomopoulos Konstantinos , Zampeli Evanthia , Axiaris Georgios , Michopoulos Spyros , Belesiotou Eleni , Banasa Maria , Maraki Sofia , Kouskoumpekou Fotini , Apostolopoulos Georgios , Stamouli Vasiliki , Prifti Heleni , Mantzaris Gerasimos J. 
Published: April 2019

Background
Inflammatory bowel disease (IBD) is an independent risk factor for Clostridium difficile infection (CDI), which is associated significantly with disease severity. We aimed to determine the rates of CDI among hospitalized IBD patients in major tertiary referral hospitals in Greece.

Patients and methods
A retrospective analysis was carried out of stool cultures from hospitalized patients investigated for diarrhea, during 2016, tested for CDI with glutamate dehydrogenase (GDH) and toxins A and B.

Results
In total, 6932 patients were tested for CDI; 894 were positive for GDH (12.89%) and 339 were also positive for C. difficile toxin (4.89%). The prevalence of CDI among all hospitalized patients was 1.6/1000 patient-days. Among these, there were 401 IBD patients, and 62 were positive for GDH (15.46%) and 30 were also positive for C. difficile toxin (7.48%). The prevalence of CDI in IBD patients was 2.5/1000 patient-days, significantly higher than in non-IBD hospitalized patients (30/401 vs. 309/6531, P=0.013). Among the 30 IBD patients (ulcerative colitis=18, Crohn’s disease=12) with CDI, six were receiving biologics, three were on corticosteroids [one combined with azathioprine (AZA) and one combined with 5-ASA], nine were on AZA monotherapy and 12 were on 5-ASA monotherapy. The prevalence of CDI among patients receiving AZA monotherapy was significantly higher than in patients receiving other medications (9/68 vs. 21/333, P=0.047). Mild CDI (n=28) was treated with metronidazole and/or vancomycin, whereas severe CDI (n=2) was treated with vancomycin.

Conclusion
The prevalence of CDI is higher in hospitalized IBD patients than those without IBD and AZA monotherapy increases the risk of CDI.
Why Some Walk and Others Don't: Neighborhood Safety and the Sociodemographic Variation Effect on Walking for Leisure and Transportation
Authors: Aliyas Zeinab 
Published: April 2019

Objective:The study was aimed at assessing the association of all components of safety (crime, traffic, social, and physical) and general safety with walking for leisure and transportation among Iranian adults. In this regard, sociodemographic factors as potential effect modifiers on walking behavior and the association of neighborhood safety with the level of walking were explored.Method:A total of 1300 questionnaires were distributed among respondents residing in 8 neighborhoods in Bandar Abbas city, among which 1132 qualified for use in further analysis. Using a binary logistic regression method, differences in walking behavior and perceived safety to walk were evaluated across sociodemographic characteristics.Results:The results showed that the walking rate is higher among older adults, married people, and adults with lower education and a higher length of residency. An increase in neighborhood safety from crime, traffic, social disorders, and overall safety associates with increased leisure walking among all populations. Further, overall neighborhood safety and safety from crime and traffic positively correlate with the level of transportation walking among all respondents. For both types of walking, this association varies in sociodemographic groups.Conclusion:The study suggests that an increase in the safety of a neighborhood contributes to a higher level of leisure and transportation walking; however, it varies in different subgroups. This study provides the knowledge needed to consider all aspects of neighborhood safety to measure walking behavior level.
Mitochondrial PE potentiates respiratory enzymes to amplify skeletal muscle aerobic capacity
Authors: Heden Timothy D. , Johnson Jordan M. , Ferrara Patrick J. , Eshima Hiroaki , Verkerke Anthony R. P. , Wentzler Edward J. , Siripoksup Piyarat , Narowski Tara M. , Coleman Chanel B. , Lin Chien-Te , Ryan Terence E. , Reidy Paul T. , de Castro Brás Lisandra E. , Karner Courtney M. , Burant Charles F. , Maschek J. Alan , Cox James E. , Mashek Douglas G. , Kardon Gabrielle , Boudina Sihem , Zeczycki Tonya N. , Rutter Jared , Shaikh Saame R. , Vance Jean E. , Drummond Micah J. , Neufer P. Darrell , Funai Katsuhiko 
Published: April 2019

AbstractExercise capacity is a strong predictor of all-cause mortality. Skeletal muscle mitochondrial respiratory capacity, its biggest contributor, adapts robustly to changes in energy demands induced by contractile activity. While transcriptional regulation of mitochondrial enzymes has been extensively studied, there is limited information on how mitochondrial membrane lipids are regulated. Herein, we show that exercise training or muscle disuse alters mitochondrial membrane phospholipids including phosphatidylethanolamine (PE). Addition of PE promoted, whereas removal of PE diminished, mitochondrial respiratory capacity. Surprisingly, skeletal muscle-specific inhibition of mitochondrial-autonomous synthesis of PE caused a respiratory failure due to metabolic insults in the diaphragm muscle. While mitochondrial PE deficiency coincided with increased oxidative stress, neutralization of the latter did not rescue lethality. These findings highlight the previously underappreciated role of mitochondrial membrane phospholipids in dynamically controlling skeletal muscle energetics and function.
Random forest-based modelling to detect biomarkers for prostate cancer progression
Authors: Toth Reka , Schiffmann Heiko , Hube-Magg Claudia , Büscheck Franziska , Höflmayer Doris , Weidemann Sören , Lebok Patrick , Fraune Christoph , Minner Sarah , Schlomm Thorsten , Sauter Guido , Plass Christoph , Assenov Yassen , Simon Ronald , Meiners Jan , Gerhäuser Clarissa 
Published: April 2019

AbstractThe clinical course of prostate cancer (PCa) is highly variable, demanding an individualized approach to therapy and robust prognostic markers for treatment decisions. We present a random forest-based classification model to predict aggressive behaviour of PCa. DNA methylation changes between PCa cases with good or poor prognosis (discovery cohort with n=70) were used as input. The model was validated with data from two large independent PCa cohorts from the “International Cancer Genome Consortium” (ICGC) and “The Cancer Genome Atlas” (TCGA). Ranking of cancer progression-related DNA methylation changes allowed selection of candidate genes for additional validation by immunohistochemistry. We identified loss of ZIC2 protein expression, mediated by alterations in DNA methylation, as a promising novel prognostic biomarker for PCa in >12,000 tissue micro-array tumors. The prognostic value of ZIC2 proved to be independent from established clinico-pathological variables including Gleason grade, tumor stage, nodal stage and PSA. In summary, we have developed a PCa classification model, which either directly orviaexpression analyses of the identified top ranked candidate genes might help in decision making related to the treatment of prostate cancer patients.
Meiotic Cellular Rejuvenation is Coupled to Nuclear Remodeling in Budding Yeast
Authors: King Grant A. , Goodman Jay S. , Chetlapalli Keerthana , Schick Jennifer G. , Jorgens Danielle M. , McDonald Kent L. , Ünal Elçin 
Published: April 2019

ABSTRACTProduction of healthy gametes in meiosis relies on the quality control and proper distribution of both nuclear and cytoplasmic contents. Meiotic differentiation naturally eliminates age-induced cellular damage by an unknown mechanism. Using time-lapse fluorescence microscopy in budding yeast, we found that nuclear senescence factors – including protein aggregates, extrachromosomal ribosomal DNA circles, and abnormal nucleolar material – are sequestered away from chromosomes during meiosis II and subsequently eliminated. A similar sequestration and elimination process occurs for the core subunits of the nuclear pore complex in both young and old cells. Nuclear envelope remodeling drives the formation of a membranous compartment containing the sequestered material. Importantly,de novogeneration of plasma membrane is required for the sequestration event, preventing the inheritance of long-lived nucleoporins and senescence factors into the newly formed gametes. Our study uncovers a new mechanism of nuclear quality control and provides insight into its function in meiotic cellular rejuvenation.
West Nile Virus Subverts T Cell Stimulatory Capacity of Human Dendritic Cells
Authors: Zimmerman Matthew G. , Bowen James R. , McDonald Circe E. , Pulendran Bali , Suthar Mehul S. 
Published: April 2019

AbstractWest Nile virus (WNV) is a neurotropic flavivirus and the leading cause of mosquito-borne encephalitis in the United States. Recent studies in humans have found that dysfunctional T cell responses strongly correlate with development of severe WNV neuroinvasive disease. However, the contributions of human dendritic cells (DCs) in priming WNV-specific T cell immunity remains poorly understood. Here, we demonstrate that human monocyte-derived DCs (moDCs) support productive viral replication following infection with a pathogenic strain of WNV. Antiviral effector gene transcription was strongly induced during the log-phase viral growth, while secretion of type I interferons (IFN) occurred with delayed kinetics. Activation of RIG-I like receptor (RLR) or type I IFN signaling prior to log-phase viral growth significantly diminished viral replication, suggesting that activation of antiviral programs early can block WNV infection. In contrast to the induction of antiviral responses, WNV infection did not promote transcription or secretion of pro-inflammatory (IL-6, GM-CSF, CCL3, CCL5, CXCL9) or T cell modulatory cytokines (IL-4, IL-12, IL-15). There was also minimal induction of molecules associated with antigen presentation and T cell priming, including the co-stimulatory molecules CD80, CD86, and CD40. Functionally, WNV-infected moDCs dampened allogenic CD4 and CD8 T cell activation and proliferation. Combined, we propose a model where WNV subverts human DC activation to compromise priming of WNV-specific T cell immunity.ImportanceWest Nile virus (WNV) is an encephalitic flavivirus that remains endemic in the United States. Previous studies have found dysfunctional T cell responses correlate to severe disease outcomes during human WNV infection. Here, we sought to better understand the ability of WNV to program human dendritic cells (DCs) to prime WNV-specific T cell responses. While productive infection of monocyte-derived DCs activated antiviral and type I interferon responses, molecules associated with inflammation and programming of T cells were minimally induced. Functionally, WNV-infected DCs dampened T cell activation and proliferation during an allogeneic response. Combined, our data supports a model where WNV infection of human DCs compromises WNV-specific T cell immunity.
Genomic Selection in Rubber Tree Breeding: A Comparison of Models and Methods for dealing with G × E
Authors: Souza L.M. , Francisco F.R. , Gonçalves P.S. , Scaloppi Junior E.J. , Le Guen V. , Fritsche-Neto R. , Souza A.P 
Published: April 2019

AbstractSeveral genomic prediction models incorporating genotype × environment (G×E) interactions have recently been developed and used in genomic selection (GS) in plant breeding programs. G×E interactions decrease selection accuracy and limit genetic gains in plant breeding. Two genomic data sets were used to compare the prediction ability of multi-environment G×E genomic models and two kernel methods (a linear kernel (genomic best linear unbiased predictor, GBLUP) (GB) and a nonlinear kernel (Gaussian kernel, GK)) and prediction accuracy (PA) of five genomic prediction models: (1) one without environmental data (BSG); (2) a single-environment, main genotypic effect model (SM); (3) a multi-environment, main genotypic effect model (MM); (4) a multi-environment, single variance GxE deviation model (MDs); and (5) a multi-environment, environment-specific variance GxE deviation model (MDe). We evaluated the utility of GS with 435 rubber tree individuals in two sites and genotyped the individuals with genotyping-by-sequencing (GBS) of single-nucleotide polymorphisms (SNPs). Prediction models were estimated for diameter (DAP) and height (AP) at different ages, with a heritability ranging from 0.59 to 0.75 for both traits. Applying the model (BSG, SM, MM, MDs, and MDe) and kernel method (GBLUP and GK) combinations to rubber tree data showed that models with the nonlinear GK and linear GBLUP kernel had similar PAs. Multi-environment models were superior to single-environment genomic models regardless the kernel (GBLUP or GK), suggesting that introducing interactions between markers and environmental conditions increases the proportion of variance explained by the model and, more importantly, the PA. In the best scenario (well-watered (WW / GK), an increase of 6.7 and 8.7 fold of genetic gain can be obtained for AP and DAP, respectively, with multi-environment GS (MM, MDe and MDS) than by conventional genetic breeding model (CBM). Furthermore, GS resulted in a more balanced selection response in DAP and AP and if used in conjunction with traditional genetic breeding programs will contribute to a reduction in selection time. With the rapid advances in and declining costs of genotyping methods, balanced against the overall costs of managing large progeny trials and potential increased gains per unit time, we are hopeful that GS can be implemented in rubber tree breeding programs.
Deciphering the mechanoresponsive role of β-catenin in Keratoconus epithelium
Authors: Amit Chatterjee , Padmanabhan Prema , Narayanan Janakiraman 
Published: April 2019

AbstractKeratoconus (KC) a disease with established biomechanical instability of the corneal stroma, is an ideal platform to identify key proteins involved in mechanosensing. This study aims to investigate the possible role of β-catenin as mechanotransducer in KC epithelium. KC patients were graded as mild, moderate or severe using Amsler Krumeich classification. Immunoblotting and tissue immunofluorescence studies were performed on KC epithelium to analyze the expression and localization of β-catenin, E-cadherin, ZO1, α-catenin, Cyclin D1, α-actinin, RhoA, Rac123. Co-immunoprecipitation (Co-IP) of β-catenin followed by mass spectrometry of mild KC epithelium was performed to identify its interacting partners. This was further validated by using epithelial tissues grown on scaffolds of different stiffness. We observed down regulation of E-cadherin, α-catenin, ZO1 and upregulation of Cyclin D1, α-actinin and RhoA in KC corneal epithelium. β-catenin Co-IP from mild KC epithelium identified new interacting partners such as StAR-related lipid transfer protein3, Dynamin-1-like protein, Cardiotrophin-1,Musculin, Basal cell adhesion molecule and Protocadherin Fat 1.β-catenin localization was altered in KC which was validatedin vitro, using control corneal epithelium grown on different substrate stiffness. β-catenin localization is dependent upon the elastic modulus of the substrate and acts as mechanotransducer by altering its interaction and regulating the barrier function in corneal epithelium.
The sensory school: working with teachers, parents and pupils to create good sensory conditions
Authors: Martin Nicola , Milton Damian Elgin Maclean , Krupa Joanna , Brett Sally , Bulman Kim , Callow Danielle , Copeland Fiona , Cunningham Laura , Ellis Wendy , Harvey Tina , Moranska Monika , Roach Rebecca , Wilmot Seanne 
Published: April 2019

Purpose
An alliance of schools and researchers formed a collaborative community of practice in order to understand and improve the sensory school environment for pupils on the autistic spectrum, and incorporate the findings into school improvement planning. The paper aims to discuss this issue.

Design/methodology/approach
Representatives of special and mainstream schools in South London and a team of researchers formed the project team, including an autistic researcher. The researchers and a named staff member from each of the schools met regularly over the course of 18 months in order to work on an iterative process to improve the sensory experience pupils had of the school environment. Each school completed sensory audits and observations, and was visited by members of the research team. Parents were involved via meetings with the research team and two conferences were organised to share findings.

Findings
Useful outcomes included: developing and sharing of good practice between schools; opportunities for parents of autistic pupils to discuss their concerns, particularly with someone with insider perspective; and exploration of creative ways to achieve pupil involvement and the idea that good autism practice has the potential to benefit all pupils. A resource pack was produced for the schools to access. Plans are in place to revisit the initiative in 12 months’ time in order to ascertain whether there have been long-term benefits.

Originality/value
Projects building communities of practice involving autistic people as core team members are rare, yet feedback from those involved in the project showed this to be a key aspect of shared learning.
What would people do with their money if they were rich? A search for Hofstede dimensions across 52 countries
Authors: Minkov Michael , Dutt Pinaki , Schachner Michael , Jandosova Janar , Khassenbekov Yerlan , Morales Oswaldo , Blagoev Vesselin 
Published: April 2019

Purpose
The purpose of this paper is to test the replicability of Hofstede’s value-based dimensions – masculinity–femininity (MAS–FEM) and individualism–collectivism (IDV–COLL) – in the field of consumer behavior, and to compare cultural prioritizations with respect to disposable income budgets across the world.

Design/methodology/approach
The authors asked 51,529 probabilistically selected respondents in 52 countries (50 nationally representative consumer panels and community samples from another two countries) what they would do with their money if they were rich. The questionnaire items targeted Hofstede’s MAS–FEM and IDV–COLL as well as a wider range of options deemed sufficiently meaningful, ethical and moral across the world.

Findings
The authors obtained two main dimensions. The first contrasts self-enhancing and altruistic choices (status and power-seeking spending vs donating for healthcare) and is conceptually similar to MAS–FEM. However, it is statistically related to Hofstede’s fifth dimension, or monumentalism–flexibility (MON–FLX), not to MAS–FEM. The second dimension contrasts conservative-collectivist choices and modern-hedonistic concerns (donating for religion and sports vs preserving nature and travel abroad for pleasure) and is a variant of COLL–IDV.

Research limitations/implications
The authors left out various potential consumer choices as they were deemed culturally incomparable or unacceptable in some societies. Nevertheless, the findings paint a sufficiently rich image of worldwide value differences underpinning idealized consumer behavior prioritizations.

Practical implications
The study could be useful to international marketing and consumer behavior experts.

Social implications
The study contributes to the understanding of modern cultural differences across the world.

Originality/value
This is the first large cross-cultural study that reveals differences in values through a novel approach: prioritizations of consumer choices. It enriches the understanding of IDV–COLL and MON–FLX, and, in particular, of the value prioritizations of the East Asian nations. The study provides new evidence that Hofstede’s MAS–FEM is a peculiarity of his IBM database with no societal analogue. Some of the so-called MAS–FEM values are components of MON–FLX, which is statistically unrelated to Hofstede’s MAS–FEM.