bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adoption rates of laparoscopic techniques for colorectal resections among Brazilian surgeons: limiting factors affecting incorporation into daily practice
Authors: Campos Fábio Guilherme , Bertoncini Alexandre Bruno , Martinez Carlos Augusto Real , Bustamante-Lopez Leonardo Alphonso , Morais Paula Gabriela Melo 
Published: November 2018

AbstractRoutine adoption of laparoscopy in clinical practice and Medical Residency has not been widely evaluated in Brazil so far.
Aim To take an overview on the adoption and limitations concerning the use of laparoscopic techniques among Brazilian colorectal surgeons.
Methods A questionnaire was sent to 1870 SBCP filiated members, containing personal and professional data such as sex, age, length and local of practice, SBCP filliation, number of procedures, treatment of cancer and laparoscopy limitations.
Results 418 members (22.4%) sent their response (80% men and 20% women). 110 members (26.3%) affirmed they don’t perform any laparoscopic procedure, while 308 (73.7%) have already adopted laparoscopy as a routine. An average number of 7.6 laparoscopic colorectal procedures were declared to be performed per month (1 to 40 procedures). Laparoscopic adoption rates were favourably influenced by young age members (46% vs. 28%) and affiliation to University hospitals (p = 0.01). Conversely, surgeons from private clinic showed a greater tendency of no adoption. Among the 308 responders, 106 (34.4%) have already surpassed more than 100 laparoscopic cases, and 167 (54.2%) reported an experience of more than 50 operated patients. The group of surgeons not using minimally invasive techniques incriminated lack of training (73.6%) and laparoscopic instruments availability (27.3%) as the main reasons for no adoption.
Conclusions Adoption rate of laparoscopic techniques to treat colorectal diseases is still low (at least 17%). Future efforts should focus on providing supervised training, proctorship during the initial experience and help instrumental acquisition in centers willing to change their routine and perspectives.
Efficacy of low residue enteral formula versus clear liquid diet during bowel preparation for colonoscopy: a randomised controlled pilot trial
Authors: Gee Tikfu , Lee Limi , Liew Ngoh Chin , Lim Shu Yu , Ghani Nur Suriyana Abd , Martindale Robert G. 
Published: November 2018

Abstract
Objectives Conventional bowel preparation for colonoscopy confines patient to clear liquid diet the day before and such non-nutritive dietary regimen often caused discomfort and hunger. The purpose of this study is to determine the feasibility of feeding patient with low-residue, lactose-free semi-elemental enteral formula (PEPTAMEN®) compare to conventional clear liquid diet during bowel preparation before colonoscopy.
Methods This was a randomised, endoscopist-blinded study. Patients were randomised into two groups, those receiving oral PEPTAMEN® and mechanical bowel preparation (A) and those receiving clear liquid while undergoing mechanical bowel preparation (B). Documentation was made with regard to the type of bowel cleansing agents used, completeness of the colonoscopy, cleanliness quality score, and hunger score.
Results A total of 97 patients were included in the study, A = 48 and B = 49. Eight patients, who were not compliant to the bowel-cleansing agent or had an incomplete colonoscopic examination, were excluded from the study. In terms of the overall cleanliness score, no statistical significant difference was seen (p = 0.25) between the two groups, A (fair or poor 37.5%, good or excellent 62.5%) and B (fair or poor 49%, good or excellent 51%) whereas the hunger score showed a significant difference (p = 0.016), A (no hunger 41.7%, slight hunger 12.5%, hungry 12.5%) and B (no hunger 24.5%, slight hunger 38.8%, hungry 36.7%).
Conclusions These data suggest that the addition of oral PEPTAMEN® as part of the bowel preparation regimen did not significantly alter the luminal cleanliness score during colonoscopy while alleviating hunger.
Local irrigation with silver nitrate, a non-surgical approach for persistent anal fistula
Authors: Placer-Galán Carlos , Aguirre-Allende Ignacio , Enriquez-Navascués José María 
Published: November 2018

Abstract
Purpose Treatment of persistent anal fistula implies a major challenge for surgeons, with risk of additional recurrence and potential continence impairment. We present a non-surgical treatment based on irrigation with silver nitrate 1% solution.
Methods This is a prospective study including patients with persistent anal fistula after surgery, who were treated with silver nitrate 1% solution irrigation from May 2015 to March 2017. Patients with evidence of abscess, presence of >1 external opening and those with bowel inflammatory disease were excluded. 3–5 cc of silver nitrate 1% solution were instilled through a catheter. The procedure was repeated on a weekly basis, conducting a maximum of 7 sessions per patient.
Results 18 patients (13 male, 72.2%) with a median age of 48 years old (IQR 41–55) were treated using silver nitrate 1% solution. A median of 5 sessions per patient was performed (IQR 3–6). The median follow-up period was 18 months (IQR 9–25). After the described period 8 patients’ (44.4%) presented complete resolution of the fistula, 2 patients’ (11.2%) were classified as partial healing and in 8 patients’ (44.4%) the treatment was considered to fail. 6 patients’ experienced self-limited pain during instillation, with persistence up to 24 h in 2 of them.
Conclusions Treatment with silver nitrate 1% solution is a minimally invasive procedure, with a favourable safety profile, that can be performed in an outpatients’ basis achieving a complete healing rate of 44.4%. Therefore, this method should be considered for the treatment of recurrent or persistent anal fistula.
Evolution of the Southern Segment of the Philippine Trench: Constraints From Seismic Tomography
Authors: Fan Jianke , Zhao Dapeng 
Published: November 2018

AbstractA high‐resolution model of three‐dimensional P wave tomography of the crust and upper mantle beneath the southern part of the Philippine Trench is determined by inverting 22,960 arrival times of 2,789 local earthquakes and 4,660 traveltime residuals of 751 teleseismic events simultaneously. The tomographic method TOMOG3D is used to obtain the tomographic model that has a lateral resolution of 0.6° or better, as shown by extensive checkerboard resolution tests. A model‐recovery synthetic test is also performed, which indicates that main features of our tomographic images are quite robust. Our results show that the Philippine Sea slab has subducted down to depths of 450–600 km with an overturned dip angle along the southern segment of the Philippine Trench, indicating that the subduction of the Philippine Sea Plate along the southern segment of the Philippine Trench initiated at 20–25 Ma, contemporaneous with the Sangihe Trench. The subduction initiation of the Philippine Sea Plate at the central Philippine Trench resulted from the collision between the Philippines and the Palawan block at ~10 Ma. The subduction completion of the Molucca Sea slab propagated from the south to the north at ~5 Ma, which presumably resulted in the subduction reorganization of the Philippine Sea Plate along the southern segment of the Philippine Trench and subduction initiation of the Philippine Sea Plate along the northern segment of the Philippine Trench.
VLF Transmitters as Tools for Monitoring the Plasmasphere
Authors: Koronczay Dávid , Lichtenberger János , Juhász Lilla , Steinbach Péter , Hospodarsky George 
Published: November 2018

AbstractContinuous burst mode very low frequency (VLF) measurements were recorded on the Van Allen Probes satellites and are analyzed to detect pulses from the Russian Alpha (RSDN‐20) ground‐based navigational system between January and March 2016. Based on the wave characteristics of these pulses and on the position of the spacecraft, the signals propagated mostly in ducted mode in the plasmasphere. Knowledge of the propagation path allowed us to carry out a monochromatic wave propagation inversion to obtain plasmaspheric electron densities. We compared the obtained densities with independent in situ measurements on the spacecraft. The results show good agreement, validating our inversion process. This contributes to validating the field‐aligned density profile model routinely used in the inversion of whistlers detected on the ground. Furthermore, our method can provide electron densities at regimes where no alternative measurements are available on the spacecraft. This raises the possibility of using this method as an additional tool to measure and monitor plasmaspheric electron densities.
Water Resources Assessment of China's Transboundary River Basins Using a Machine Learning Approach
Authors: Yan Jiabao , Jia Shaofeng , Lv Aifeng , Zhu Wenbin 
Published: November 2018

AbstractA comprehensive and reliable assessment of the water resources in China's transboundary river basins is vital for water resources management and peaceful development. In this study, we built machine learning (random forest, gradient boosting, and stacking) and traditional linear models to identify the relation between the runoff coefficient and its influencing factors, including topography, climate, land cover, and soil. The cross‐validation results show that the machine learning models greatly outperform the traditional linear model in predicting runoff coefficient. High‐resolution (0.1°) runoff coefficient and runoff maps for the China's transboundary river basins riparian countries were produced and compared with other estimates at the country level. The best water resources estimates achieved from the machine learning model are consistent with the Food and Agriculture Organization of the United Nations AQUASTAT database (root‐mean‐square error = 76.97 km3/year, normalized root‐mean‐square error = 12%) at the country level. This outperformed two currently available runoff products: the UNH/GRDC Global Composite Runoff Fields and the Global Streamflow Characteristics Dataset. The study also demonstrated that accurate precipitation data can improve runoff and water resources estimation accuracy and that climate and topographic factors have a controlling role in prediction, whereas the influences of land cover and soils are weak. Finally, China's transboundary water resources were calculated and thoroughly assessed at basin and country levels.
Deformation twinning and grain partitioning in a hexagonal close-packed magnesium alloy
Authors: Arul Kumar M. , Clausen B. , Capolungo L. , McCabe R. J. , Liu W. , Tischler J. Z. , Tomé C. N. 
Published: November 2018

AbstractPervasive deformation twinning in magnesium greatly affects its strength and formability. The local stress fields associated with twinning play a key role on deformation behavior and fracture but are extremely difficult to characterize experimentally. In this study, we perform synchrotron experiments with differential-aperture X-ray microscopy to measure the 3D stress fields in the vicinity of a twin with a spatial resolution of 0.5 micrometer. The measured local stress field aids to identify the sequence of events involved with twinning. We find that the selected grain deforms elastically before twinning, and the twin formation splits the grain into two non-interacting domains. Under further straining one domain of the grain continued to deform elastically, whereas the other domain deforms plastically by prismatic slip. This heterogeneous deformation behavior may be mediated by the surrounding medium and it is likely to lead to asymmetric twin growth.
Pressure shock fronts formed by ultra-fast shear cracks in viscoelastic materials
Authors: Gori M. , Rubino V. , Rosakis A. J. , Lapusta N. 
Published: November 2018

AbstractSpontaneously propagating cracks in solids emit both pressure and shear waves. When a shear crack propagates faster than the shear wave speed of the material, the coalescence of the shear wavelets emitted by the near-crack-tip region forms a shock front that significantly concentrates particle motion. Such a shock front should not be possible for pressure waves, because cracks should not be able to exceed the pressure wave speed in isotropic linear-elastic solids. In this study, we present full-field experimental measurements of dynamic shear cracks in viscoelastic polymers that result in the formation of a pressure shock front, in addition to the shear one. The apparent violation of classic theories is explained by the strain-rate-dependent material behavior of polymers, where the crack speed remains below the highest pressure wave speed prevailing locally around the crack tip. These findings have important implications for the physics and dynamics of shear cracks such as earthquakes.
Calcium stabilizes the strongest protein fold
Authors: Milles Lukas F. , Unterauer Eduard M. , Nicolaus Thomas , Gaub Hermann E. 
Published: November 2018

AbstractStaphylococcal pathogens adhere to their human targets with exceptional resilience to mechanical stress, some propagating force to the bacterium via small, Ig-like folds called B domains. We examine the mechanical stability of these folds using atomic force microscopy-based single-molecule force spectroscopy. The force required to unfold a single B domain is larger than 2 nN – the highest mechanostability of a protein to date by a large margin. B domains coordinate three calcium ions, which we identify as crucial for their extreme mechanical strength. When calcium is removed through chelation, unfolding forces drop by a factor of four. Through systematic mutations in the calcium coordination sites we can tune the unfolding forces from over 2 nN to 0.15 nN, and dissect the contribution of each ion to B domain mechanostability. Their extraordinary strength, rapid refolding and calcium-tunable force response make B domains interesting protein design targets.
Downregulation of VEGFR3 signaling alters cardiac lymphatic vessel organization and leads to a higher mortality after acute myocardial infarction
Authors: Vuorio Taina , Ylä-Herttuala Elias , Laakkonen Johanna P. , Laidinen Svetlana , Liimatainen Timo , Ylä-Herttuala Seppo 
Published: November 2018

AbstractHeart has a wide lymphatic network but the importance of cardiac lymphatic system in heart diseases has remained unclear. Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) is a key molecule in the development and maintenance of cardiac lymphatic vessels. Here we characterized the role of VEGFR3 in healthy hearts and after myocardial infarction (MI) by using sVEGFR3 transgenic mice expressing a soluble decoy VEGFR3 under K14 promoter and Chy mice which have an inactivating mutation in the VEGFR3 gene. Cardiac lymphatic vessels were significantly dilated in the healthy hearts of sVEGFR3 mice when compared to controls. Lymphatic vessels formed large sheet-like structures in Chy mice. Attenuated VEGFR3 signaling led to a more severe MI predisposing to a significantly higher mortality in sVEGFR3 mice than in control mice. sVEGFR3 mice displayed intramyocardial hemorrhages in the infarcted area indicating hyperpermeability of the vasculature. Furthermore, novel MRI methods TRAFF2 and TRAFF4 and histological analysis revealed a modified structure of the fibrotic infarcted area in sVEGFR3 mice. In conclusion, the downregulation of VEGFR3 signaling modifies the structure of cardiac lymphatic network and causes vascular leakiness and increased mortality after MI.
Design and effectiveness evaluation of mirror myoelectric interfaces: a novel method to restore movement in hemiplegic patients
Authors: Sarasola-Sanz Andrea , Irastorza-Landa Nerea , López-Larraz Eduardo , Shiman Farid , Spüler Martin , Birbaumer Niels , Ramos-Murguialday Ander 
Published: November 2018

AbstractThe motor impairment occurring after a stroke is characterized by pathological muscle activation patterns or synergies. However, while robot-aided myoelectric interfaces have been proposed for stroke rehabilitation, they do not address this issue, which might result in inefficient interventions. Here, we present a novel paradigm that relies on the correction of the pathological muscle activity as a way to elicit rehabilitation, even in patients with complete paralysis. Previous studies demonstrated that there are no substantial inter-limb differences in the muscle synergy organization of healthy individuals. We propose building a subject-specific model of muscle activity from the healthy limb and mirroring it to use it as a learning tool for the patient to reproduce the same healthy myoelectric patterns on the paretic limb during functional task training. Here, we aim at understanding how this myoelectric model, which translates muscle activity into continuous movements of a 7-degree of freedom upper limb exoskeleton, could transfer between sessions, arms and tasks. The experiments with 8 healthy individuals and 2 chronic stroke patients proved the feasibility and effectiveness of such myoelectric interface. We anticipate the proposed method to become an efficient strategy for the correction of maladaptive muscle activity and the rehabilitation of stroke patients.
Crystallographic and kinetic analyses of human IPMK reveal disordered domains modulate ATP binding and kinase activity
Authors: Seacrist Corey D. , Blind Raymond D. 
Published: November 2018

AbstractInositol polyphosphate multikinase (IPMK) is a member of the IPK-superfamily of kinases, catalyzing phosphorylation of several soluble inositols and the signaling phospholipid PI(4,5)P2 (PIP2). IPMK also has critical non-catalytic roles in p53, mTOR/Raptor, TRAF6 and AMPK signaling mediated partly by two disordered domains. Although IPMK non-catalytic functions are well established, it is less clear if the disordered domains are important for IPMK kinase activity or ATP binding. Here, kinetic and structural analyses of an engineered human IPMK lacking all disordered domains (ΔIPMK) are presented. Although the KM for PIP2 is identical between ΔIPMK and wild type, ΔIPMK has a 1.8-fold increase in kcat for PIP2, indicating the native IPMK disordered domains decrease IPMK activity in vitro. The 2.5 Å crystal structure of ΔIPMK is reported, confirming the conserved ATP-grasp fold. A comparison with other IPK-superfamily structures revealed a putative “ATP-clamp” in the disordered N-terminus, we predicted would stabilize ATP binding. Consistent with this observation, removal of the ATP clamp sequence increases the KM for ATP 4.9-fold, indicating the N-terminus enhances ATP binding to IPMK. Together, these structural and kinetic studies suggest in addition to mediating protein-protein interactions, the disordered domains of IPMK impart modulatory capacity to IPMK kinase activity through multiple kinetic mechanisms.
Identification of DKK-1 as a novel mediator of statin effects in human endothelial cells
Authors: Pontremoli Marta , Brioschi Maura , Baetta Roberta , Ghilardi Stefania , Banfi Cristina 
Published: November 2018

AbstractThis study shows that DKK-1, a member of the Dickkopf family and a regulator of the Wnt pathways, represents a novel target of statins which, through the inhibition of HMG-CoA reductase and of non-steroidal isoprenoid intermediates, exert extra-beneficial effect in preventing atherosclerosis beyond their effect on the lipid profile. We found that atorvastatin downregulates DKK-1 protein (−88.3 ± 4.1%) and mRNA expression (−90 ± 4.2%) through the inhibition of Cdc42, Rho and Rac geranylgeranylated proteins. Further, a combined approach based on the integration of label-free quantitative mass spectrometry based-proteomics and gene silencing allowed us to demonstrate that DKK-1 itself mediates, at least in part, statin effects on human endothelial cells. Indeed, DKK-1 is responsible for the regulation of the 21% of the statin-modulated proteins, which include, among others, clusterin/apoJ, plasminogen activator inhibitor type 1 (PAI-1), myristoylated alanine-rich C-kinase substrate (MARCKS), and pentraxin 3 (PTX3). The Gene Ontology enrichment annotation revealed that DKK-1 is also a potential mediator of the extracellular matrix organization, platelet activation and response to wounding processes induced by statin. Finally, we found that plasma level of DKK-1 from cholesterol-fed rabbits treated with atorvastatin (2.5 mg/kg/day for 8 weeks) was lower (−42 ± 23%) than that of control animals. Thus, DKK-1 is not only a target of statin but it directly regulates the expression of molecules involved in a plethora of biological functions, thus expanding its role, which has been so far restricted mainly to cancer.
Enabling precision medicine in neonatology, an integrated repository for preterm birth research
Authors: Sirota Marina , Thomas Cristel G. , Liu Rebecca , Zuhl Maya , Banerjee Payal , Wong Ronald J. , Quaintance Cecele C. , Leite Rita , Chubiz Jessica , Anderson Rebecca , Chappell Joanne , Kim Mara , Grobman William , Zhang Ge , Rokas Antonis , Muglia Louis J. , Ober Carol , England Sarah K. , Macones George , Driscoll Deborah , Parry Samuel , Shaw Gary M. , Stevenson David K. , Simpson Joe Leigh , Thomson Elizabeth , Butte Atul J. ,   March of Dimes Prematurity Research Centers, Driscoll Deborah , Macones George , Muglia Louis J , Ober Carole , Stevenson David K. 
Published: November 2018

AbstractPreterm birth, or the delivery of an infant prior to 37 weeks of gestation, is a significant cause of infant morbidity and mortality. In the last decade, the advent and continued development of molecular profiling technologies has enabled researchers to generate vast amount of ‘omics’ data, which together with integrative computational approaches, can help refine the current knowledge about disease mechanisms, diagnostics, and therapeutics. Here we describe the March of Dimes’ Database for Preterm Birth Research (http://www.immport.org/resources/mod), a unique resource that contains a variety of ‘omics’ datasets related to preterm birth. The database is open publicly, and as of January 2018, links 13 molecular studies with data across tens of thousands of patients from 6 measurement modalities. The data in the repository are highly diverse and include genomic, transcriptomic, immunological, and microbiome data. Relevant datasets are augmented with additional molecular characterizations of almost 25,000 biological samples from public databases. We believe our data-sharing efforts will lead to enhanced research collaborations and coordination accelerating the overall pace of discovery in preterm birth research.
X-ray computed microtomography of Megachirella wachtleri
Authors: Simões Tiago R. , Caldwell Michael W. , Tałanda Mateusz , Bernardi Massimo , Palci Alessandro , Vernygora Oksana , Bernardini Federico , Mancini Lucia , Nydam Randall L. 
Published: November 2018

AbstractUnderstanding the origin and early evolution of squamates has been a considerable challenge given the extremely scarce fossil record of early squamates and their poor degree of preservation. In order to overcome those limitations, we conducted high-resolution X-ray computed tomography (CT) studies on the fossil reptile Megachirella wachtleri (Middle Triassic, northern Italy), which revealed an important set of features indicating this is the oldest known fossil squamate in the world, predating the previous oldest record by ca. 75 million years. We also compiled a new phylogenetic data set comprising a large sample of diapsid reptiles (including morphological and molecular data) to investigate the phylogenetic relationships of early squamates and other reptile groups along with the divergence time of those lineages. The re-description of Megachirella and a new phylogenetic hypothesis of diapsid relationships are presented in a separate study. Here we present the data descriptors for the tomographic scans of Megachirella, which holds fundamental information to our understanding on the early evolution of one of the largest vertebrate groups on Earth today.