bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short communication: Estimation of the dietary standardized ileal digestible valine to lysine ratio required for 40 to 130 kg pigs during the finisher periods
Authors: Wu Zijuan , Li Wenli , Li Yali 
Published: November 2024

Abstract
Background
Three experiments were conducted separately to determine the optimum standardized ileal digestible (SID) valine (Val) to lysine (Lys) ratio for early finishing (Experiment 1, 40 to 75 kg bodyweight), finishing (Experiment 2, 75 to 100 kg bodyweight), and late finishing (Experiment 3, 100 to 130 kg bodyweight) pigs. Dietary SID Val: Lys ratios were designed at 0.61, 0.65, 0.69, 0.73, and 0.77. The optimal SID Val: Lys ratio was estimated by different regression models, including a quadratic polynomial model, a two-slope quadratic broken-line model, a curvilinear-plateau model, and a one-slope straight broken-line model.

Results
In Exp.1, a total of 550 early finishing pigs (initially 40.3 kg bodyweight) were used in a 38-day growth trial. Pigs consuming 0.61 dietary SID Val: Lys ratio had lower final bodyweight compared to those fed 0.69 in diets. Using regression models, the optimal dietary SID Val: Lys requirement for average daily gain (ADG) was between 0.63 and 0.68, and for feed to gain ratio (F: G) was between 0.62 and 0.68, respectively. In Exp.2, 525 finishing pigs (initially 76.4 kg) were used in a 26-day trial. Based on regression models, estimate of the required SID Val: Lys for ADG was between 0.65 and 0.71, and for F: G was between 0.64 and 0.70, respectively. In Exp.3, 640 late finishing pigs (102 kg bodyweight) were used in a 27-day trial. No significant improvement was found for performance parameters of pigs from 100 to 130 kg, while 0.73 SID Val: Lys ratio resulted in the highest ADG and the lowest F: G from a numerical point of view.

Conclusions
These findings indicated that the optimum SID Val: Lys requirement for pigs from 40 to 75 kg was between 0.62 and 0.68, and for pigs from 75 to 100 kg was estimated to be between 0.64 and 0.71, using different regression models.
PEG600 induced krill oil-based nanoemulsion system: ternary phase behaviour and cytotoxicity assessment
Authors: Sharma Anshika , Saifi Arshad , Kumar Anoop 
Published: November 2024

Abstract
Background
Endogenous substances of krill oil (KO) are lipophilic in nature and have clinical significance viz. DHA/EPA, phospholipids and astaxanthin. To improve the nanodispersibility of endogenous substances of KO, a self-nanoemulsifying system (SNE) was developed.

Results
Ternary phase behaviour of KO was explored in ethanol (ET); propylene glycol, (PG); and PEG600 using Tween80 and Tween20 as surfactants. PEG600 induced the self-nanoemulsification of KO and yielded one phase region (OPR); dilution lines (KO/Smix fraction containing PEG600) traversed across OPR, produced a fully dilutable nanoemulsion system. PEG600-based nanoformulations (NFs) of KO underwent phase transformation via percolation behaviour in nanostructure domains (86–207 nm). PEG600 induced ternary phase behaviour of KO as revealed from rheological data (higher eta values), refractive index (nonlinear) and conductivity (bimodal) patterns. Induced phase transformation could be an interaction between aqueous phase and KO/Tween20 in PEG600 environment; generating highly viscous domains of low electrical conductivity. NFs offered antioxidant activity over corresponding coarse systems (p < 0.01) as measured using DPPH method. Optimized NFs (F4 and F6) inhibited the growth of skin cancer cell line (A431) in the range of 100–500 × dilutions.

Conclusion
Phase behaviour of KO was induced by PEG600, transforming the dilution pattern via generation of one phase region; however, ethanol and propylene glycol as co-solvents did not. PEG600-based NFs of KO possessed antioxidant as well as cytotoxic to skin cancer cell lines (A431).
Long-Term Outcomes of Stereotactic Radiosurgery for Pineocytomas: An International Multicenter Study
Authors: Hamel Andréanne , Tourigny Jean-Nicolas , Niranjan Ajay , Lunsford L. Dade , Wei Zhishuo , Srinivasan Priyanka N. , Liscak Roman , May Jaromir , Martínez Moreno Nuria , Martínez Álvarez Roberto , Lee Cheng-chia , Yang Huai-che , Tripathi Manjul , Kumar Narendra , Mashiach Elad , Kondziolka Douglas , Briggs Robert G. , Yu Cheng , Zada Gabriel , Franzini Andrea , Pecchioli Guido , Bowden Gregory N. , Dayawansa Samantha , Sheehan Jason , Mathieu David 
Published: November 2024

BACKGROUND AND OBJECTIVES:
Pineocytomas are grade 1 tumors arising from the pineal parenchyma. Gross total resection can potentially cure these benign lesions but can be associated with morbidity. This study was designed to provide multi-institutional data to evaluate the results of stereotactic radiosurgery (SRS) for pineocytomas.

METHODS:
Centers participating in the International Radiosurgery Research Foundation were asked to review their database and provide data for patients who had SRS for histology confirmed grade 1 pineocytomas, for whom clinical and imaging follow-up of at least 6 months was available.

RESULTS:
In total, 38 patients underwent SRS as part of the management of a pineocytoma. The median age at SRS was 39 years (range 8-76). SRS was performed as primary approach in 68%, adjuvant after partial resection 19%, and at recurrence in 13% of patients. The median margin dose was 15 Gy (range 11-25 Gy). The median treatment volume was 3.35 cc (range 0.1-17.9 cc). Local tumor control was achieved in 92% of patients, with a mean actuarial progression-free survival of 21.6 years (median not reached). At last follow-up, 82% were still controlled, 8% had local recurrence, and 10% had cerebrospinal fluid dissemination. Tumor control was significantly better when SRS was used as primary care compared with the adjuvant or recurrent setting (P = .016). Five patients (13%) died during follow-up, all from tumor progression. The actuarial mean survival duration was 24.3 years, with a 5-year survival rate of 91%, and an estimated rate of 76% at 29 years. Larger tumor volume at SRS was found to be correlated to increased risk of death (P = .045). Transient symptomatic adverse radiation effects were observed in 4 patients (11%).

CONCLUSION:
SRS appears safe and effective for the management of pineocytomas. Long-term tumor control is achieved in most cases. SRS can be offered to selected patients as an alternative to surgical resection.
Outcomes Associated With Stereotactic Radiosurgery After Multiple Resections of Nonfunctioning Pituitary Macroadenomas: An International, Multicenter Case Series
Authors: Grogan Dayton , Dumot Chloe , Mantziaris Georgios , Tos Salem M , Tewari Anant , Dayawansa Sam , Sheehan Kimball , Sheehan Darrah , Peker Selcuk , Samanci Yavuz , Nabeel Ahmed M. , Reda Wael A. , Tawadros Sameh R. , AbdelKarim Khaled , El-Shehaby Amr M. N. , Emad Reem M. , Abdelsalam Ahmed Ragab , Liscak Roman , May Jaromir , Mashiach Elad , Vasconcellos Fernando De Nigris , Bernstein Kenneth , Kondziolka Douglas , Speckter Herwin , Mota Ruben , Brito Anderson , Bindal Shray Kumar , Niranjan Ajay , Lunsford L. Dade , Benjamin Carolina Gesteira , Almeida Timoteo Abrantes de Lacerda , Mathieu David , Tourigny Jean-Nicolas , Tripathi Manjul , Palmer Joshua David , Mao Jennifer , Matsui Jennifer , Crooks Joseph , Wegner Rodney E. , Shepard Matthew J. , Sheehan Jason 
Published: November 2024

BACKGROUND AND OBJECTIVES:Stereotactic radiosurgery (SRS) represents an effective treatment for nonfunctioning pituitary adenomas (NFPAs). However, no data have yet been published regarding results of SRS on NFPAs after multiple previous resections.METHODS:Retrospective multicentric data of patients diagnosed with NFPA and who underwent multiple resections (≥2) before SRS were reviewed and analyzed. The treatment interval spanned the period of 1992 to 2022. Cox regression and Kaplan–Meier curves were used to assess predictive factors and the probability of tumor control and hypopituitarism.RESULTS:Among the 311 patients (median age: 50.2 [IQR: 18.0] years), 226 (72.7%) had undergone ≥2 previous resections. The median margin dose was 14 Gy (IQR: 4.0 Gy), and the median tumor volume 3.6 cm3(IQR: 4.8). Overall, the probability of tumor control after SRS was 93.3% (CI 95%: 89.9-96.9) and 86.7% (CI 95%: 81.1-92.6) at 5 and 10 years, respectively. A margin dose >14 Gy was associated with a decreased risk of tumor progression (hazard ratio = 0.33, CI 95% = 0.15-0.75,P= .008). At a last clinical follow-up of 4.1 (IQR 6.1) years, 10.1% (30/296) developed at least 1 new hormone deficiency after SRS. The cumulative probability of new hormone deficiency was 6.1% (95% CI: 3.0-9.1), 10.3% (95% CI: 5.8-14.6), and 18.9% (95% CI: 11.5-25.8) at 3, 5, and 10 years after SRS, respectively. The average latency between SRS and development of new hormone deficiencies was 3.3 years (IQR 4.1). A maximum point dose to the pituitary stalk >10 Gy was associated with a new deficiency (hazard ratio = 4.06, CI 95% = 1.57-10.5,P-value = .004).CONCLUSION:For patients with NFPA with multiple previous resections, SRS offers effective local tumor control and a low risk of delayed hypopituitarism for managing these challenging adenomas. SRS should be strongly considered in patients with NFPA with 2 previous resections compared with considering a third resection.
A framework for the dissemination of research on depression via social media
Authors: Jackson Nathan , Cockrill Antje , Almoraish Ahmed 
Published: November 2024

Background/Aims Effective dissemination of research findings can help to ensure that information reaches the relevant stakeholders, informing evidence-based practice. However, the dissemination of mental health research via widely used channels, such as social media, can be challenging. This study explored the perspectives of researchers (experts) and research users (stakeholders) on the dissemination of depression-related research via social media, aiming to use these insights to create a framework to guide this process. Methods Participants were identified using the lead author's professional network. Semi-structured interviews were conducted with five experts (including researchers and one communications officer) and five stakeholders (including mental health practitioners and a member of the public). Interviews were recorded and transcribed verbatim. Data were analysed using thematic analysis. Results Experts and stakeholder groups felt that social media could be a useful tool for disseminating research, although this was not always a priority for experts. Barriers to effective dissemination via social media included gaps in expertise, difficulties measuring impact, resource limitations, concerns about negative feedback and differences in stakeholder preferences. Suggestions to optimise dissemination included engaging stakeholders; building knowledge of stakeholders and working with communications teams. Participants generally felt that there was a need for guidance on these processes. The authors used this feedback to create a framework for the dissemination of depression-related research via social media. Conclusions Mental health researchers and stakeholders are aware of the potential benefits of social media for the dissemination of depression-related research, but may require more guidance to overcome barriers to effective use of these platforms. A framework could be a useful way of providing this guidance and ensuring that research findings reach key stakeholders.
Pan-TRK positive uterine sarcoma in immunohistochemistry without neurotrophic tyrosine receptor kinase gene fusions: A case report
Authors: Lee Seungmee , Jeon Yu-Ra , Shin Changmin , Kwon Sun-Young , Shin Sojin 
Published: November 2024

BACKGROUND
The classification of uterine sarcomas is based on distinctive morphological and immunophenotypic characteristics, increasingly supported by molecular genetic diagnostics. Data on neurotrophic tyrosine receptor kinase (NTRK ) gene fusion-positive uterine sarcoma, potentially aggressive and morphologically similar to fibrosarcoma, are limited due to its recent recognition. Pan-TRK immunohistochemistry (IHC) analysis serves as an effective screening tool with high sensitivity and specificity for NTRK -fusion malignancies.
CASE SUMMARY
We report a case of a malignant mesenchymal tumor originating from the uterine cervix, which was pan-TRK IHC-positive but lacked NTRK gene fusions, accompanied by a brief literature review. A 55-year-old woman presented to the emergency department with abdominal pain and distension, exhibiting significant ascites and multiple solid pelvic masses. Pelvic examination revealed a tumor encompassing the uterine cervix, extending to the vagina and uterine corpus. A punch biopsy of the cervix indicated NTRK sarcoma with positive immunochemical pan-TRK stain. However, subsequent next generation sequencing revealed no NTRK gene fusion, leading to a diagnosis of poorly differentiated, advanced-stage sarcoma.
CONCLUSION
The clinical significance of NTRK gene fusion lies in potential treatment with TRK inhibitors for positive sarcomas. Identifying such rare tumors is crucial due to the potential applicability of tropomyosin receptor kinase inhibitor treatment.
A good index surgery for congenital pseudarthrosis of the tibia minimizes complications following surgery
Authors: Tan Si H. S. , Pei Yingzhi , Chan Chloe X. , Pang Khang C. , Lim Andrew K. S. , Hui James H. , Ning Bo 
Published: November 2024

AimsCongenital pseudarthrosis of the tibia (CPT) has traditionally been a difficult condition to treat, with high complication rates, including nonunion, refractures, malalignment, and leg length discrepancy. Surgical approaches to treatment of CPT include intramedullary rodding, external fixation, combined intramedullary rodding and external fixation, vascularized fibular graft, and most recently cross-union. The current study aims to compare the outcomes and complication rates of cross-union versus other surgical approaches as an index surgery for the management of CPT. Our hypothesis was that a good index surgery for CPT achieves union and minimizes complications such as refractures and limb length discrepancy.MethodsA multicentre study was conducted involving two institutions in Singapore and China. All patients with CPT who were surgically managed between January 2009 and December 2021 were included. The patients were divided based on their index surgery. Group 1 included patients who underwent excision of hamartoma, cross-union of the tibia and fibula, autogenic iliac bone grafting, and internal fixation for their index surgery. Group 2 included patients who underwent all other surgical procedures for their index surgery, including excision of hamartoma, intramedullary rodding, and/or external fixation, without cross-union of the tibia and fibula. Comparisons of the rates of union, refracture, limb length discrepancy, reoperations, and other complications were performed between the two groups.ResultsA total of 36 patients were included in the study. Group 1 comprised 13 patients, while Group 2 comprised 23 patients. The mean age at index surgery was four years (1 to 13). The mean duration of follow-up was 4.85 years (1.75 to 14). All patients in Group 1 achieved bony union at a mean of three months (1.5 to 4), but ten of 23 patients in Group 2 had nonunion of the pseudarthrosis (p = 0.006). None of the patients in Group 1 had a refracture, while seven of 13 patients who achieved bony union in Group 2 suffered a refracture (p = 0.005). None of the patients in Group 1 had a limb length discrepancy of more than 2 cm, while ten of 23 patients in Group 2 have a limb length discrepancy of more than 2 cm (p = 0.006). In Group 1, four of 13 patients had a complication, while 16 of 23 patients in Group 2 had a complication (p = 0.004). Excluding removal of implants, four of 13 patients in Group 1 had to undergo additional surgery, while 18 of 23 patients in Group 2 had to undergo additional surgery following the index surgery (p = 0.011).ConclusionA good index surgery of excision of hamartoma, cross-union of the tibia and fibula, autogenic iliac bone grafting, and internal fixation for CPT achieves union and minimizes complications such as refractures, limb length discrepancy, and need for additional surgeries.
Optical characterization of a manual tunable lens for eye-care applications
Authors: Salvador-Roger Raquel , Esteve-Taboada José J. , Micó Vicente 
Published: November 2024

The popularity of focus tunable lenses has increased in the last decade. In this study we present an experimental optical characterization of a commercially available manually tunable lens to describe its behavior regarding optical aberrations, expressed in terms of Zernike coefficients, under different laboratory conditions. Measurements were performed by using a Shack–Hartmann aberrometer, and four different experiments were carried out in order to assess 1) the lens stability in time for a given temperature, 2) the temporal response of the lens, 3) the behavior of the lens when changing the room temperature, and 4) the possible influence of gravity on the lens performance according to its mounting orientation. The main conclusion we outlined states that the properties of the tunable lens stay steady over time as long as room temperature remains constant, making it a good option for ophthalmologic and optometric eye-care applications.
First evidence of circulation of multiple arboviruses in Algeria
Authors: Boubidi Saïd C. , Mousson Laurence , Kernif Tahar , Khardine Fayez , Hachid Aïssam , Beck Cécile , Lecollinet Sylvie , Moraes Rayane A. , Moutailler Sara , Dauga Catherine , Failloux Anna Bella 
Published: November 2024

Background
Algeria like other North African countries is experiencing recurrent episodes of West Nile Virus (WNV) emergences and new health threats associated with the introduction of Aedes albopictus in 2010 are to be feared. To improve the surveillance of mosquito-borne pathogens, we performed a study using innovative tools based on multiplex molecular methods.

Methods
We combined two approaches: a high-throughput chip based on the BioMark Dynamic array system to detect arboviruses in mosquitoes, and a set of immunologic methods (ELISA, microsphere immunoassays (MIA) and virus microneutralization tests (MNT)) for serological surveys in animal hosts. We investigated two distinct regions: a first zone located in the coastal humid region and a second one in the Saharan desert region.

Principal findings
We collected a total of 1,658 mosquitoes belonging to nine different species and found predominantly Culex pipienss. l. (56.5%) and Cx. perexiguus (27.5%). From 180 pools of 10 mosquitoes, we detected four arboviruses: Banna virus (BAV), chikungunya virus (CHIKV), Sindbis virus (SINV), and Usutu virus (USUV). Moreover, we examined 389 blood samples from equids and poultry and found that 52.4% were positive for flavivirus antibodies in ELISA, while 30.8% were positive for WNV and two chickens and two equids were positive for USUV by MNT and MIA respectively.

Conclusions
To our knowledge, this is the first report of five arboviruses circulating in Algeria, with three reported for the first time (CHIKV, BAV, and USUV). Our study brings evidence that reinforcing surveillance using more discriminant tools may help in anticipating future emergences and propose adapted control measures.
Understanding the success factors of MOOCs’ retention intention: A Necessary Condition Analysis
Authors: Huacui Zhang , Hossain Md. Nahin , Zhen Kong , Kumar Nilesh 
Published: November 2024

Purpose
This paper seeks to explore the influence of success factors, specifically motivation and course quality, on MOOC retention intention. Going beyond a mere examination of these motivational and quality factors, the study investigates students’ motivation, considering needs, interests, course system, content, and service quality. Methodologically, a questionnaire survey was conducted, collecting data from 311 students enrolled in online courses. To ascertain the impact of interest or need-based motivation on students’ retention rates, a Structural Equation Model (SEM) was employed. Subsequently, Necessary Condition Analysis (NCA) was utilized to identify the essential factors and components in each area. SEM results revealed a positive influence of motivational factors and quality issues on students’ behavior. Retention behavior was notably affected by academic and professional needs, along with personal interests. Furthermore, course content and service quality demonstrated a significant effect on students’ perseverance behavior. NCA results identified academic motivation and system quality as having a substantial impact on retention behavior, while personal motivation and technological motivation had a comparatively smaller effect. Practically, the findings suggest that course developers should consider students’ academic and personal requirements when designing online courses. Additionally, providing students with the ability to customize course and system content according to their needs is crucial. Timely problem-solving attitudes from service providers are essential for ensuring student retention.
Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins
Authors: Ruan Chenyao , Wang Chen , Gu Jiawen , Zhu Zhihui 
Published: November 2024

Objective
Isoscopoletin is one of the primary metabolites of natural product scoparone, which was reported to against tumor proliferation. The aim of this study was to explore the mechanism of isoscopoletin against hepatocellular carcinoma (HCC).

Methods
Transcriptomics was used to reveal the possible pathways of isoscopoletin against HCC in vitro. The potential targets of isoscopoletin against HCC through affecting glycolysis were analyzed by network pharmacology, then the potential binding abilities of isoscopoletin to glycolysis-related proteins were initially verified by high throughput virtual molecular docking. The affinities of isoscopoletin for glycolysis-related proteins were assayed using microscale thermophoresis (MST), which was reverse-validated by inhibiting the binding ability of isoscopoletin to GPD2. Glucose consumption and lactate production were examined to evaluate the effects of isoscopoletin on intracellular glycolysis, and the regulation of glycolysis-related targets by isoscopoletin was detected using RT-qPCR and ELISA kits.

Results
The results of transcriptomics showed that the differentially expressed genes (DEGs) were mainly enriched in glycolysis and other metabolic-related pathways. Network pharmacology and molecular docking revealed that GPD2, GPI, HSP90AA1 and PGK2 were the core targets in the glycolysis process of isoscopoletin against HCC. MST results showed that there was a strong affinity between isoscopoletin and GPD2, GPI, Hsp90α and PGK2. In vitro results showed that isoscopoletin inhibited glucose consumption and lactate production, while regulating the levels of glycolysis-related proteins.

Conclusion
This study suggests that isoscopoletin may exist an anti-tumor effect by regulating the glycolysis-related proteins GPD2, GPI, Hsp90α and PGK2, inhibiting the glycolysis process in HCC cells, then blocking the energy supply of tumor cells.