bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sociality genes are associated with human-directed social behaviour in golden and Labrador retriever dogs
Authors: Persson Mia E. , Sundman Ann-Sofie , Halldén Lise-Lotte , Trottier Agaia J. , Jensen Per 
Published: November 2018

Background
Dogs have human-directed social skills that allow them to communicate and cooperate with humans. We have previously identified two loci on chromosome 26 associated with human contact-seeking behaviors during an unsolvable problem task in laboratory beagles (Persson et al., 2016). The aim of the present study was to verify the single nucleotide polymorphisms (SNPs) in additional dog breeds. We also studied how the allele frequencies have changed during domestication and recent selection.

Methods
Dogs of two breeds, 61 golden retrievers and 100 Labrador retrievers, were phenotyped and genotyped, and 19 wolves were genotyped. The Labrador retrievers were divided into common and field type by pedigree data to make it possible to study the effects of recent selection. All dogs were tested in an unsolvable problem task where human-directed social behaviors were scored. DNA from dogs (buccal swabs) and wolves (blood or brain tissue) was analyzed for genotype on two of the previously identified SNP markers, BICF2G630798942 (SNP1) and BICF2S23712114 (SNP2), by pyrosequencing.

Results
There was genetic variation for SNP1 in both dog breeds whereas the wolves were fixed for this polymorphism, and for SNP2 there was variation in both dogs and wolves. For both SNPs, Labrador retriever types differed significantly in allele frequencies. We found associations between SNPs and human-directed social behavior in both dog breeds. In golden retrievers, SNP1 was associated with physical contact variables, for example, with the duration of physical contact with the owner (F2,56 = 4.389, p = 0.017). SNP2 was associated with several behavioral variables in both breeds, among others owner gazing frequency in both golden retrievers (F2,55 = 6.330, p = 0.003) and Labradors (F1,93 = 5.209, p = 0.025).

Discussion
Our results verify the association between the previously identified SNPs and human-directed social behavior scored in an unsolvable problem task. Differences in allele frequencies suggest that these loci have been affected by selection. The results indicate that these genomic regions are involved in human-directed social behavior in not only beagles but in other dog breeds as well. We hypothesize that they may have been important during dog domestication.
Initiating Global Service Learning Movement: Best Practices from Petra Christian University
Authors: Intan Rolly 
Published: November 2018

Petra Christian University (PCU) is a private Christian university established in September 22nd, 1961. Tracing back its history when there was a significant necessity in providing higher education opportunity and service in Surabaya, especially for Christian and Chinese-Indonesian people; PCU was initiated to fulfill the needs. As a population of academic community, up to present the majority of PCU student body are Chinese-Indonesian descendants with middle level-economic family background.
Through its vision “To be a Caring and Global University with Commitment to the Christian Values”, PCU enhances its concern and commitment in applying the “whole person education” or “holistic education” to equip the students with at least five excellences (academic excellence, emotional excellence, moral excellence, spiritual excellence and cultural excellence). Hence, the mind, heart, spirit and cultural engagement are blended nicely within their learning process in the university to prepare their roles successfully in the global era as global citizens. PCU, then, learns that Service-Learning (S-L) program is one of the most effective and powerful concepts as well as learning method in order to achieve the holistic education objective. In the program, students as participants gain some experience not only to mingle amongst the rural communities, but also to purposely support the society’s life by sharing their expertise, involving their emotion in building relationships and communication, and earning personal reflection and commitment to continue the caring spirit toward others.
Association between cirrhosis and aneurysmal subarachnoid hemorrhage
Authors: Parikh Neal S. , Merkler Alexander E. , Jesudian Arun , Kamel Hooman 
Published: November 2018

AbstractObjectiveCirrhosis has been associated with nontraumatic subarachnoid hemorrhage (SAH). We sought to evaluate the specific association between cirrhosis and aneurysmal SAH.MethodsWe performed a retrospective cohort study using a sample of Medicare claims data from 2008 to 2015. Cirrhosis was defined using a validated ICD‐9‐CM diagnosis code algorithm. Nontraumatic SAH was identified using a validated approach requiring an inpatient claim for its ICD‐9‐CM diagnosis code. Additionally, we required the presence of an ICD‐9‐CM procedure code reflecting treatment of a cerebral aneurysm during the same hospitalization to ensure ascertainment of aneurysmal SAH specifically. We used survival statistics to calculate incidence rates and Cox proportional hazards models to evaluate the association between cirrhosis and aneurysmal SAH after adjustment for demographics, stroke risk factors, and comorbidities.ResultsWe identified 10,658 (0.6%) patients with cirrhosis from among the 1,778,604 beneficiaries in our sample. The mean age of patients with cirrhosis was 73.5 (±7.8) years, and 48% were female. Over a mean of 4.7 (±2.1) years of follow‐up, 4,272 patients were hospitalized with aneurysmal SAH. The annual incidence of aneurysmal SAH in patients with cirrhosis was 0.12% (95% confidence interval [CI], 0.08–0.17%) compared to 0.05% (95% CI, 0.05–0.05%) in patients without cirrhosis. In the adjusted model, cirrhosis was independently associated with aneurysmal SAH (hazard ratio, 2.2; 95% confidence interval, 1.5–3.4).InterpretationCirrhosis was independently associated with an increased risk of aneurysmal SAH among older individuals. Confirmation of these findings may yield opportunities for risk stratification and prevention.
Surfactant Free Delivery of Docetaxel by Poly[(R)‐3‐hydroxybutyrate‐(R)‐3‐hydroxyhexanoate]‐Based Polymeric Micelles for Effective Melanoma Treatments
Authors: Luo Zheng , Jiang Lu , Ding Chizhu , Hu Benhui , Loh Xian Jun , Li Zibiao , Wu Yun‐Long 
Published: November 2018

AbstractDocetaxel (DTX) is a new semisynthetic chemical in the taxoid family and serves a wide spectrum of chemotherapeutics. Current commercial formulation of DTX is based on the addition of the nonionic surfactants (i.e., ethanol and Tween 80), which are reported to cause severe hemolysis, hypersensitivity reactions, or neurotoxic toxicity and greatly hinders patient tolerance or compliance. In this report, a novel low‐toxic, biodegradable, and amphiphilic poly[(R)‐3‐hydroxybutyrate‐(R)‐3‐hydroxyhexanoate] (PHBHx)‐based polyurethane (a copolymer made of hydrophobic PHBHx with biocompatible D‐3‐hydroxybutyric acid as degradation product, thermosensitive polypropylene glycol (PPG), and hydrophilic polyethylene glycol (PEG) segments) with nanosized micelle formation ability to encapsulate DTX, as a surfactant free formulation, is reported. Interestingly, this DTX‐loaded poly(PHBHx/PEG/PPG urethane) micelle formulation with >90% drug loading efficiency shows significantly improved DTX solubility in aqueous medium, reduced hemolysis for better blood compatibility, and increased drug uptake in A375 melanoma cells, which provides the possibility of systematic delivery of DTX. As a proof‐of‐concept, an A375 melanoma xenograft mouse model is established to verify the therapeutic effect of this DTX‐loaded poly(PHBHx/PEG/PPG urethane) micelle formulation, indicating the promising application of PHBHx‐based polymeric nanosized micelle as a surfactant free formulation of chemotherapeutics which might greatly be beneficial for controllable delivery of pharmaceutics and cancer therapy.
Direct Patterning of Piezoelectric Thin Films by Inkjet Printing
Authors: Godard Nicolas , Glinšek Sebastjan , Matavž Aleksander , Bobnar Vid , Defay Emmanuel 
Published: November 2018

AbstractInkjet printing is currently gaining momentum as a low‐cost and eco‐friendly additive manufacturing technique for electronics. Patterning of functional metal oxide thin films on high surface energy substrates via inkjet printing remains a challenge and requires assistance from lithographic processes. This work unveils a novel process for lithography and etching‐free patterning of lead zirconate titanate (PZT) films on pristine platinized silicon through the use of inkjet‐printed alkanethiolate‐based templates. The technique requires neither lithography nor etching, respectively, before and after PZT printing. The described process allows for feature sizes in the sub‐100 µm range with control over the thickness of the final film. Inkjet‐printed PZT displays typical ferroelectric and piezoelectric properties of solution‐derived thin films, with remnant polarization 13 µC cm−2, coercive field 58 kV cm−1, permittivity 900, dielectric losses 0.07, and effective longitudinal piezoelectric coefficient 50 pm V−1.
Rapid On‐Chip Healing of Metal Thin Films
Authors: Danzi Stefano , Schnabel Volker , Gabl Johannes , Sologubenko Alla , Galinski Henning , Spolenak Ralph 
Published: November 2018

AbstractSelf‐healing behavior, the ability to autonomously counteract damage, is observed in some inorganic materials, and it has recently been extended to various artificial systems. In metals, healing usually requires thermal activation by a furnace treatment that stimulates damage repair. High temperature exposure, however, renders these routes incompatible with temperature‐sensitive systems such as on‐chip microelectronic components. In this work, designing Ni/Al multilayers as on‐chip heat sources, a concept for on‐demand healing of metal films that no longer relies on external annealing, is demonstrated. The process is based on harvesting a solitary self‐sustained heat wave that is produced by a solid‐state reaction in the heat source to weld cracks in different metal films. Healing is activated at room temperature with a remarkably small current input and in situ probing reveals a large conductance recovery up to 500 nm wide cracks within 1 ms, orders of magnitude faster than furnace‐based approaches. Intrinsic heat source healing represents a unique concept for rapid on‐chip healing of metal films that will provide new flexibility to prevent failure in inaccessible electronic systems: from implantable healthcare devices, to space probe instrumentation.
CO2 Capture in Metal–Organic Framework Adsorbents: An Engineering Perspective
Authors: Hu Zhigang , Wang Yuxiang , Shah Bhuvan B. , Zhao Dan 
Published: November 2018

AbstractCO2 emission has raised worldwide concerns because of its potential effects on climate change, species extinction, and plant nutrition deterioration. Metal–organic frameworks (MOFs) are one class of crystalline adsorbent materials that are believed to be of huge potential in CO2 capture applications because of their advantages such as ultrahigh porosity, boundless chemical tunability, and surface functionality over traditional porous zeolites and activated carbon. In terms of chemistry, there are already many studies devoted to the synthesis of new functional MOFs. Some of the synthesized MOFs have been evaluated for CO2 capture at laboratory‐scale. Several reviews have been published on this topic, but mainly from a chemistry and materials point of view. In this review, the authors focus on the engineering perspective on this topic, with emphases on material evaluation, performance judgment, and process design to address the engineering issues of these materials to be used as adsorbents in industrial CO2 capture. The current engineering evaluation approaches for MOFs are summarized, in a manner that could also be applied to other adsorbent materials.
Thermionic Emission–Based Interconnecting Layer Featuring Solvent Resistance for Monolithic Tandem Solar Cells with Solution‐Processed Perovskites
Authors: Li Can , Wang Zi Shuai , Zhu Hugh Lu , Zhang Di , Cheng Jiaqi , Lin Hong , Ouyang Dan , Choy Wallace C. H. 
Published: November 2018

AbstractAll‐perovskite tandem cells have been considered a potential candidate for bringing the power conversion efficiency (PCE) beyond the Shockley–Queisser limit of single‐junction device while retaining the advantages of earth‐abundant materials and solution processability. However, a challenging issue with regard to realizing such solution‐processed devices is the fulfillment of complex and coupled requirements of the interconnecting layer (ICL), including solvent resistance to protect underlying perovskite film, high electrical properties for carrier transport and recombination, and high optical transmission. In this work, a new thermionic emission–based ICL with enhanced solvent resistance features is demonstrated. Fundamentally, the thermionic emission plays a critical role in the electron transport process in the ICL, which is confirmed through both experimental and theoretical studies. Besides achieving high optical transmission and electrical properties, the new ICL chemically protects the underlying perovskite film by introducing a fluoride silane–incorporated polyethylenimine ethoxylated hybrid system that also passivates the surface defects to reduce electrical loss. The monolithic all‐perovskite tandem cells demonstrate highest PCE of 17.9% (from current density–voltage scan) and the highest steady‐state efficiency is 16.1% for a typical device. Consequently, this work contributes to not only understanding the fundamental mechanism of ICLs but also promotes robust and low‐cost photovoltaics.
Arylierungschemie für die Biokonjugation
Authors: Zhang Chi , Vinogradova Ekaterina V. , Spokoyny Alexander M. , Buchwald Stephen L. , Pentelute Bradley L. 
Published: November 2018

AbstractMittels Biokonjugationschemie können modifizierte Biomoleküle hergestellt werden, deren Funktionsumfang das natürliche Maß übersteigt. Hierbei ist die Entwicklung hocheffizienter und selektiver Biokonjugationsreaktionen von Bedeutung, die unter milden, biokompatiblen Bedingungen ablaufen. Vielversprechend sind hierbei Verfahren, bei denen Bindungen zwischen einem Nukleophil und einem sp2‐hybridisierten Kohlenstoffatom gebildet werden, wodurch in manchen Fällen Moleküle mit einzigartigen Eigenschaften erhalten werden können. In diesem Aufsatz werden Biokonjugationsstrategien für die Arylierung von Schwefel, Stickstoff, Selen, Sauerstoff und Kohlenstoff sowie ihre Anwendungen für die Modifikation von Peptiden, Proteinen, Kohlenhydraten und Nukleinsäuren zusammengefasst.
Tin Diselenide Molecular Precursor for Solution‐Processable Thermoelectric Materials
Authors: Zhang Yu , Liu Yu , Lim Khak Ho , Xing Congcong , Li Mengyao , Zhang Ting , Tang Pengyi , Arbiol Jordi , Llorca Jordi , Ng Ka Ming , Ibáñez Maria , Guardia Pablo , Prato Mirko , Cadavid Doris , Cabot Andreu 
Published: November 2018

AbstractIn the present work, we detail a fast and simple solution‐based method to synthesize hexagonal SnSe2 nanoplates (NPLs) and their use to produce crystallographically textured SnSe2 nanomaterials. We also demonstrate that the same strategy can be used to produce orthorhombic SnSe nanostructures and nanomaterials. NPLs are grown through a screw dislocation‐driven mechanism. This mechanism typically results in pyramidal structures, but we demonstrate here that the growth from multiple dislocations results in flower‐like structures. Crystallographically textured SnSe2 bulk nanomaterials obtained from the hot pressing of these SnSe2 structures display highly anisotropic charge and heat transport properties and thermoelectric (TE) figures of merit limited by relatively low electrical conductivities. To improve this parameter, SnSe2 NPLs are blended here with metal nanoparticles. The electrical conductivities of the blends are significantly improved with respect to bare SnSe2 NPLs, what translates into a three‐fold increase of the TE Figure of merit, reaching unprecedented ZT values up to 0.65.
Enantioselective Organocatalytic Four‐Atom Ring Expansion of Cyclobutanones: Synthesis of Benzazocinones
Authors: Zhou Yirong , Wei Yun‐Long , Rodriguez Jean , Coquerel Yoann 
Published: November 2018

AbstractAn enantioselective Michael addition– four‐atom ring expansion cascade reaction involving cyclobutanones activated by a N‐aryl secondary amide group and ortho‐amino nitrostyrenes has been developed for the preparation of functionalized eight‐membered benzolactams using bifunctional aminocatalysts. Taking advantage of the secondary amide activating group, the eight‐membered cyclic products could be further rearranged into their six‐membered isomers having a glutarimide core under base catalysis conditions without erosion of optical purity, featuring an overall ring expansion– ring contraction strategy.
Confined Pt11+ Water Clusters in a MOF Catalyze the Low‐Temperature Water–Gas Shift Reaction with both CO2 Oxygen Atoms Coming from Water
Authors: Rivero‐Crespo Miguel A. , Mon Marta , Ferrando‐Soria Jesús , Lopes Christian W. , Boronat Mercedes , Leyva‐Pérez Antonio , Corma Avelino , Hernández‐Garrido Juan C. , López‐Haro Miguel , Calvino Jose J. , Ramos‐Fernandez Enrique V. , Armentano Donatella , Pardo Emilio 
Published: November 2018

AbstractThe synthesis and reactivity of single metal atoms in a low‐valence state bound to just water, rather than to organic ligands or surfaces, is a major experimental challenge. Herein, we show a gram‐scale wet synthesis of Pt11+ stabilized in a confined space by a crystallographically well‐defined first water sphere, and with a second coordination sphere linked to a metal–organic framework (MOF) through electrostatic and H‐bonding interactions. The role of the water cluster is not only isolating and stabilizing the Pt atoms, but also regulating the charge of the metal and the adsorption of reactants. This is shown for the low‐temperature water–gas shift reaction (WGSR: CO + H2O → CO2 + H2), where both metal coordinated and H‐bonded water molecules trigger a double water attack mechanism to CO and give CO2 with both oxygen atoms coming from water. The stabilized Pt1+ single sites allow performing the WGSR at temperatures as low as 50 °C.
Arylation Chemistry for Bioconjugation
Authors: Zhang Chi , Vinogradova Ekaterina V. , Spokoyny Alexander M. , Buchwald Stephen L. , Pentelute Bradley L. 
Published: November 2018

AbstractBioconjugation chemistry has been used to prepare modified biomolecules with functions beyond what nature intended. Central to these techniques is the development of highly efficient and selective bioconjugation reactions that operate under mild, biomolecule compatible conditions. Methods that form a nucleophile–sp2 carbon bond show promise for creating bioconjugates with new modifications, sometimes resulting in molecules with unparalleled functions. Here we outline and review sulfur, nitrogen, selenium, oxygen, and carbon arylative bioconjugation strategies and their applications to modify peptides, proteins, sugars, and nucleic acids
An investigation of the molecular and biochemical basis underlying chlorantraniliprole‐resistant Drosophila strains and their cross‐resistance to other insecticides
Authors: Kim A‐Young , Kwon Deok Ho , Jeong In Hong , Koh Young Ho 
Published: November 2018

AbstractChlorantraniliprole is an anthranilic diamide insecticide that binds to the insect ryanodine receptor (RyR) and induces an uncontrolled release of Ca2+, resulting in paralysis and ultimately death of the target insects. Recently, it was reported that chlorantraniliprole‐resistant diamondback moths,
Plutella xylostella Linnaeus, have mutations in their RyR. In this study, we developed two different chlorantraniliprole‐resistant
Drosophila melanogaster strain. The resistance ratio (RR) of the low‐concentration chlorantraniliprole‐treated resistant (Low‐Res) strain was 2.3, while that of the high‐concentration chlorantraniliprole‐treated resistant (High‐Res) strain was 21.3. The LC
50 of the untreated control (Con) strain was 23.8~25.9 ppm, which was significantly higher than that reported for the susceptible diamondback moth (0.03~0.51 ppm). The high LC
50 of the Con may be because the helix S2 amino acid sequence of
D. melanogaster RyR (
DmRyR) is identical to the I4790M mutation of the chlorantraniliprole‐resistant diamondback moths, resulting in a lower binding affinity of
DmRyR for chlorantraniliprole. Among the tested detoxification enzymes, the activity of esterase was significantly increased in the two Res strains, but glutathione
S‐transferases and acetylcholinesterase were significantly decreased in the two Res strains. The cross‐resistance of the High‐Res strain to other insecticides with different modes of actions (MoAs) revealed that the RRs of the neuronal acetylcholine receptor allosteric and competitive modulators were significantly increased, while those of the Na
2+ channel modulators were significantly reduced. Our studies showed that RRs against the same insecticide vary with the treatment concentration, and that RRs against other insecticides with different MoAs can be altered.
Global Folding of a Na+‐Specific DNAzyme Studied by FRET
Authors: He Yanping , Zhou Yibo , Chen Da , Liu Juewen 
Published: November 2018

AbstractRecently, a few RNA‐cleaving DNAzymes have been isolated with excellent specificity for Na+, and some of them contain a Na+‐binding aptamer. This metal recognition mechanism is different from that of most previously reported DNAzymes. When using 2‐aminopurine (2AP) as a probe, interesting local folding induced by Na+ was recently observed. In this work, FRET was used to probe the global folding of the Ce13d DNAzyme; one of the Na+‐specific DNAzymes. FRET pairs were at different locations, which yielded a total of five constructs to probe the three‐way junction structure with a large loop. With endlabeled DNAzymes, the global structure appears to be quite rigid with little folding upon adding up to 200 mm monovalent metal ions, although some minor differences were observed between Li+, Na+, and K+. This lack of significant conformational change is also consistent with circular dichroism spectroscopy data. The loop was then labeled with an internal tetramethylrhodamine fluorophore at the G14 position, and its cleavage activity was partially retained. A clear Na+‐dependent folding was observed with spectral crossover. From a biosensing standpoint, global folding based sensors are unlikely to work due to the overall rigid structure of the DNAzyme. Therefore, the best way to use this DNAzyme to discriminate Na+ from K+ is based on cleavage activity, followed by probing local folding, whereas global folding is the least effective for metal discrimination.
CYP109E1 from Bacillus megaterium Acts as a 24‐ and 25‐Hydroxylase for Cholesterol
Authors: Putkaradze Natalia , Litzenburger Martin , Hutter Michael Christopher , Bernhardt Rita 
Published: November 2018

AbstractIn this study, the ability of CYP109E1 from Bacillus megaterium DSM319 to metabolize cholesterol was investigated. This steroid was identified as a new substrate to be converted by CYP109E1 with adrenodoxin and adrenodoxin reductase as redox partners in vitro. The biotransformation was successfully reproduced in vivo by using Bacillus megaterium cells that overexpressed CYP109E1. To enhance the production of cholesterol derivatives, an Escherichia coli based whole‐cell system that harbored CYP109E1 was established. This novel system showed a 3.3‐fold higher activity than that of the B. megaterium system, yielding about 45 mg L−1 of these products. Finally, the reaction products were isolated and identified to be the highly important cholesterol derivatives 24(S)‐ and 25‐hydroxycholesterol.