bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chronic infections can shape epidemic exposure: Pathogen co-occurrence networks in the Serengeti lions
Authors: Fountain-Jones Nicholas M. , Packer Craig , Jacquot Maude , Blanchet F. Guillaume , Terio Karen , Craft Meggan E. 
Published: July 2018

AbstractPathogens are embedded in a complex network of microparasites that can collectively or individually alter disease dynamics and outcomes. Chronic pathogens, for example, can either facilitate or compete with subsequent pathogens thereby exacerbating morbidity and mortality. Pathogen interactions are ubiquitous in nature, but poorly understood, particularly in wild populations. We report here on ten years of serological and molecular data in African lions, leveraging comprehensive demographic and behavioral data to utilize pathogen networks to test if chronic infections shape infection by acute pathogens. We combine network and community ecology approaches to assess broad network structure and characterize associations between pathogens across spatial and temporal scales. We found significant non-random structure in the lion-pathogen co-occurrence network and identified potential facilitative and competitive interactions between acute and chronic pathogens. Our results provide a novel insight for untangling the complex associations underlying pathogen co-occurrence networks.
Neuronal overexpression ofDYRK1A/minibrainalters motor decline, neurodegeneration and synaptic plasticity inDrosophila
Authors: Lowe Simon A , Usowicz Maria M , Hodge James JL 
Published: July 2018

AbstractDown syndrome (DS) is characterised by abnormal cognitive and motor development, and later in life by progressive Alzheimer’s disease (AD)-like dementia, neuropathology, declining motor function and shorter life expectancy. It is caused by trisomy of chromosome 21 (Hsa21), but how individual Hsa21 genes contribute to various aspects of the disorder is incompletely understood. Previous work has demonstrated a role for triplication of the Hsa21 geneDYRK1Ain cognitive and motor deficits, as well as in altered neurogenesis and neurofibrillary degeneration in the DS brain, but its contribution to other DS phenotypes is unclear. Here we demonstrate that overexpression ofminibrain(mnb), theDrosophilaortholog ofDYRK1A, in theDrosophilanervous system accelerated age-dependent decline in motor performance and shortened lifespan. Overexpression ofmnbin the eye was neurotoxic and overexpression in ellipsoid body neurons in the brain caused age-dependent neurodegeneration. At the larval neuromuscular junction, an established model for mammalian central glutamatergic synapses, neuronalmnboverexpression enhanced spontaneous vesicular transmitter release. It also slowed recovery from short-term depression of evoked transmitter release induced by high-frequency nerve stimulation and increased the number of boutons in one of the two glutamatergic motor neurons innervating the muscle. These results provide further insight into the roles ofDYRK1Atriplication in abnormal aging and synaptic dysfunction in DS.Author summaryDown syndrome (DS) is caused by three copies of chromosome 21 instead of the usual two. It is characterised by cognitive and motor deficits, which worsen with age resulting in Alzheimer’s disease (AD). Which genes on chromosome 21 cause these phenotypes is incompletely understood. Here we demonstrate that neuronal overexpression ofminibrain, theDrosophilaortholog of the chromosome 21 geneDYRK1A, causes age-dependent degeneration of brain neurons, accelerates age-dependent decline in motor performance and shortens lifespan. It also modifies presynaptic structure, enhances spontaneous transmitter release and slows recovery from short-term depression of synaptic transmission at a model glutamatergic synapse. These findings give insight into the role ofDYRK1Aoverexpression in aberrant aging and altered information processing in DS and AD.
A post-translational modification of the sheath modulatesFrancisellatype VI secretion system assembly and function
Authors: Ziveri Jason , Chhuon Cerina , Jamet Anne , Prigent Guénolé , Rytter Héloïse , Tros Fabiola , Barel Monique , Coureuil Mathieu , Lays Claire , Henry Thomas , Keep Nicholas H , Guerrera Ida Chiara , Charbit Alain 
Published: July 2018

AbstractFrancisella tularensisis a facultative intracellular pathogen that causes the zoonotic disease tularemia in human and animal hosts. This bacterium possesses a non-canonical type VI secretion systems (T6SS) required for phagosomal escape and access to its replicative niche in the cytosol of infected macrophages. KCl stimulation has been previously used to trigger assembly and secretion of the Francisella T6SS in culture. We found that the amounts of essentially all the TSS6 proteins remained unchanged upon KCl stimulation. We therefore hypothesized that a post-translational modification might be involved in T6SS assembly. A whole cell phosphoproteomic analysis allowed us to identify a unique phosphorylation site on IglB, the TssC homologue and key component of the T6SS sheath. Importantly, the phosphorylated form of IglB was not present in the contracted sheath and 3D modeling indicated that the charge repulsion provoked by addition of a phosphogroup on tyrosine 139 was likely to weaken the stability of the sheath structure. Substitutions of the phosphorylatable residue of IglB (tyrosine 139) with alanine or with phosphomimetics prevented T6SS formation and totally impaired phagosomal escape. In contrast, the substitution with the non-phosphorylatable aromatic analog phenylalanine impaired but did not prevent phagosomal escape and cytosolic bacterial multiplication in J774-1 macrophages. Altogether these data suggest that phosphorylation of the sheath participates to T6SS disassembly. Post-translational modifications of the sheath may represent a previously unrecognized mechanism to finely modulate the dynamics of T6SS assembly-disassembly.Data are available via ProteomeXchange with identifier PXD012507.SynopsisFrancisellapossesses a non-canonical T6SS that is essential for efficient phagosomal escape and access to the cytosol of infected macrophages. KCl stimulation has been previously used to trigger assembly and secretion of the Francisella T6SS in culture. We found that KCl stimulation did not result in an increased production of TSS6 proteins. We therefore hypothesized that a post-translational modification might be involved in T6SS assembly. Using a global and site-specific phosphoproteomic analysis ofFrancisellawe identified a unique phosphorylation site on IglB, the TssC homologue and a key component of the T6SS contractile sheath. We show that this site plays a critical role in T6SS biogenesis and propose that phosphorylation may represent a new mechanism affecting the dynamics of sheath formation.
HiPLA: High-throughput imaging Proximity Ligation Assay
Authors: Serebryannyy Leonid A. , Misteli Tom 
Published: July 2018

AbstractProtein-protein interactions are essential for cellular structure and function. To delineate how the intricate assembly of protein interactions contribute to cellular processes in health and disease, new methodologies that are both highly sensitive and can be applied at large scale are needed. Here, we develop HiPLA (high-throughput imaging proximity ligation assay), a method that employs the antibody-based proximity ligation assay in a high-throughput imaging screening format to systematically probe protein interactomes. Using HiPLA, we probe the interaction of 60 proteins and associated PTMs with the nuclear lamina in a model of the premature aging disorder Hutchinson-Gilford progeria syndrome (HGPS). We identify a subset of proteins that differentially interact with the nuclear lamina in HGPS. In combination with quantitative indirect immunofluorescence, we find that the majority of differential interactions were accompanied by corresponding changes in expression of the interacting protein. Taken together, HiPLA offers a novel approach to probe cellular protein-protein interaction at a large scale and reveals mechanistic insights into the assembly of protein complexes.
Effect of laser power on amorphous forming capability and corrosion performance of laser thermal sprayed Al-Ni-Fe-Gd coatings
Authors: Baoxu Yan , Dejun Kong 
Published: July 2018

Purpose
The amorphous Al-Ni-Fe-Gd coatings were fabricated to improve anti-corrosion performance of offshore platforms.

Design/methodology/approach
The amorphous Al-Ni-Fe-Gd coatings were first fabricated on S355 steel using the laser thermal spraying.

Findings
The amorphous forming capability and corrosion resistance increases with the laser powers increasing.

Research Limitations/implications
The amorphous Al-Ni-Fe-Gd coatings were applied on S355 steel of offshore platforms to increase its long-term heavy and anti-corrosion protection.

Originality/value
The amorphous Al-Ni-Fe-Gd coatings were first fabricated using a laser thermal spraying, improving its anti-corrosion.
Project scheduling with time, cost and risk trade-off using adaptive multiple objective differential evolution
Authors: Tran Duc Hoc , Long Luong Duc 
Published: July 2018

PurposeAs often in project scheduling, when the project duration is shortened to reduce total cost, the total float is lost resulting in more critical or nearly critical activities. This, in turn, results in reducing the probability of completing the project on time and increases the risk of schedule delays. The objective of project management is to complete the scope of work on time, within budget in a safe fashion of risk to maximize overall project success. The purpose of this paper is to present an effective algorithm, named as adaptive multiple objective differential evolution (DE) for project scheduling with time, cost and risk trade-off (AMODE-TCR).Design/methodology/approachIn this paper, a multi-objective optimization model for project scheduling is developed using DE algorithm. The AMODE modifies a population-based search procedure by using adaptive mutation strategy to prevent the optimization process from becoming a purely random or a purely greedy search. An elite archiving scheme is adopted to store elite solutions and by aptly using members of the archive to direct further search.FindingsA numerical construction project case study demonstrates the ability of AMODE in generating non-dominated solutions to assist project managers to select an appropriate plan to optimize TCR problem, which is an operation that is typically difficult and time-consuming. Comparisons between the AMODE and currently widely used multiple objective algorithms verify the efficiency and effectiveness of the developed algorithm. The proposed model is expected to help project managers and decision makers in successfully completing the project on time and reduced risk by utilizing the available information and resources.Originality/valueThe paper presented a novel model that has three main contributions: First, this paper presents an effective and efficient adaptive multiple objective algorithms named as AMODE for producing optimized schedules considering time, cost and risk simultaneously. Second, the study introduces the effect of total float loss and resource control in order to enhance the schedule flexibility and reduce the risk of project delays. Third, the proposed model is capable of operating automatically without any human intervention.
The policy influence on the development of entrepreneurship in higher education
Authors: Hägg Gustav , Schölin Tobias 
Published: July 2018

Purpose
The purpose of this paper is to describe and analyze the foundation of higher education policies that have promoted entrepreneurship in Sweden since the mid-1990s.

Design/methodology/approach
To do this, the authors use Bacchi’s (1999) “What’s the problem?” approach. A central assumption of which is that perceptions of a problem affect how its solution looks. Bacchi’s approach is described as a type of discourse analysis.

Findings
The authors show that problem definition within policies regarding the role and importance of entrepreneurship within higher education has explicitly been directed toward equipping individuals to develop action-orientated skills in the field of entrepreneurship. The equipment of action-oriented skills has implicitly been directed to individuals’ personal initiatives to meet explicit social and collective problems, fueling a neoliberal development and fostering an enterprising culture. The authors also show how policy creates a discourse, which may be characterized as “useful, unreflective citizens.”

Research limitations/implications
The study addresses the implicit steering that is being exercised through policies. This steering needs to be questioned and problematized in order to avoid blindly following the implied course of action.

Originality/value
The study contributes to current understanding of how entrepreneurship in higher education is both governed explicitly and implicitly, by policy, through the creation of new norms in society.
Positioning in prospective secondary English teachers’ annotations of teaching videos
Authors: Sherry Michael B. , Messier-Jones Lauriann M. , Morales Joanelle 
Published: July 2018

PurposeEnglish education researchers have used video annotation to connect theory to practice and to encourage prospective secondary English teachers (PSETs) to reflectively evaluate their own and others’ teaching. This study aimed to examine whether and how PSETs’ annotations of their own and others’ teaching videos reveal (dis)connections between visions of English teaching valued in methods courses and those practiced in local school field placements.Design/methodology/approachExamination of 538 annotations on 18 lesson videos – recorded in a university teaching-methods course and in local secondary classrooms by 12 PSETs in a rural, northeastern US teacher-preparation program – revealed what kinds of practices PSETs evaluated and with whom they identified (student or teacher) as they made those evaluations.FindingsAnnotations from two PSETs illustrate a trend in the larger data sets: PSETs’ annotations expressed pedagogical values that differed and sometimes conflicted according to their identification with the role of student or of teacher. PSETs’ preferences as students were often superseded by visions of what one must do/be in the secondary English classroom.Research limitations/implicationsThis study identifies tensions among PSETs’ annotations that corresponded to their identifications with the role of student or of teacher but does not explore whether and how they reconciled these tensions, or how they might affect student learning. Future research might explore how PSETs negotiate contradictions in their pedagogical preferences as they annotate their own and others’ teaching videos.Practical implicationsEnglish teacher educators who use video-based methods might attend to whether and how such assignments/activities position PSETs as students or as teachers in viewing teaching videos.Originality/valuePSETs may value different and even conflicting pedagogical practices, regardless of setting and despite their own experiences, based on their identifications with the role of student or of teacher. These identifications may allow them to compartmentalize visions of teaching that might otherwise come into productive conflict.
The significance of reflective conversations for adolescent writers
Authors: Vetter Amy , Meacham Mark 
Published: July 2018

PurposeFor writing instruction, reflection has been an essential tool. Typically, educators ask students to reflect in a structured written, individual format. Less explored is the role that small and whole group reflective conversations have in fostering students’ understandings about writing. The purpose of this paper is to explore several conversations from a young writers’ camp to examine how three high school students engaged in four different kinds of reflective talk during the writing process.Design/methodology/approachThis paper draws from a larger qualitative study about how campers constructed and enacted their writer identities in a two-week young writers’ camp. Five researchers observed, video/audio recorded, engaged in interviews and collected artifacts with 58 campers for ten consecutive days. Qualitative analysis was used to examine how young writers (Grades 9-12) engaged in reflective talk to develop understandings about writing.FindingsData illustrated that students engaged in four types of reflective talk: prospective, reflective-in-action, introspective and retrospective. The paper provides one example for each kind of reflective conversation and provides analysis related to how those conversations shaped campers’ understandings about writing.Originality/valueThis paper illustrated how adolescent writers used prospective, reflective-in action, introspective and retrospective talk during conversations to tell their stories of learning about writing, a topic less studied in the field. This work offers insight into teaching students how to have such reflective conversations so that they are productive and supportive during writing practices.
Impact of reinforcing fabric thread count on cation exchange membrane properties
Authors: Stránská Eliška , Neděla David , Křivčík Jan , Weinertová Kristýna , Václavíková Natália 
Published: July 2018

Purpose
The purpose of this paper is to examine the impact of thread count in polyester reinforcing fabric on heterogeneous cation exchange membrane mechanical properties and electrochemical performance.

Design/methodology/approach
Seven polyester fabrics differing in thread count were used for membrane manufacture and mechanical properties such as ultimate force or ultimate strain of all fabrics and membranes were determined. Electrochemical and physical properties of produced membranes were evaluated as well.

Findings
It was found that with increasing weft density ultimate force became greater in the case of fabric and membrane as well. The impact of weft density on ultimate strain was not confirmed but changes in swelling ability mainly in width direction were observed. The assumption of worse electrochemical properties of membranes reinforced by fabric with lower open area was also validated and these membranes exhibited higher areal resistance.

Originality/value
Gained information is a useful tool in design process of new ion exchange membrane types with improved mechanical and swelling properties.
The effect of weave pattern on the thermal properties of woven fabrics
Authors: Asayesh Azita , Talaei Mehraneh , Maroufi Mohammad 
Published: July 2018

PurposeFabric structural parameters play an important role on the thermal comfort of clothing. The purpose of this paper is to investigate the effect of weave pattern and also the length of warp float in each weave pattern on the thermal properties of woven fabrics.Design/methodology/approachCotton woven fabrics with 23 different weave patterns were produced with identical linear densities of warp and weft yarns as well as constant warp and weft nominal densities. Thereafter, their thermal properties were studied.FindingsStatistical analysis demonstrated that the weave pattern significantly influences on the thermal properties of woven fabrics. Plain fabric exhibited the lowest thermal resistance and the highest thermal conductivity, and hopsack 2/2(4) weave fabric demonstrated the highest thermal resistance and the lowest thermal conductivity. Moreover, except hopsack (4) weave fabric, in all weave patterns, the length of warp float had a significant effect on the thermal characteristics of the fabrics, as increasing the warp float led to increase in the thermal resistance of the fabrics.Originality/valueWeave pattern as one of the structural parameters of the fabric has a determinant role on the thermal properties of fabric and subsequently, the comfort of clothing produced from it. Owing to the lack of investigation in this area, this research considers the effect of weave pattern and the length of warp float in each weave pattern on the thermal properties of woven fabrics.
Technological intensity and innovation capability in industrial firms
Authors: Zawislak Paulo Antônio , Fracasso Edi Madalena , Tello-Gamarra Jorge 
Published: July 2018

Purpose
Over time, technological intensity has been used as a proxy for innovation capability of firms in an industrial sector. However, not only firms belonging to the stratum of high technological intensity are able to innovate. Therefore, this study aims to explore a potential association between technological intensity and innovation capability in firms from different industrial sectors, using the Organization for Economic Cooperation and Development (OECD)’s classification and the components of innovation capability proposed by Zawislak et al. (2012, 2013).

Design/methodology/approach
The authors conducted an exploratory research with four case studies focusing on the innovation capability of Brazilian firms.

Findings
The results show that the four firms, each belonging to one stratum of technological intensity, have innovation capability, and the differences regarding this feature can be explained by the balance and development of all firms’ capabilities (technological, operational, managerial and transactional).

Originality/value
In the literature, studies that relate technological intensity and innovation capability are scarce. Therefore, the originality of this research is to relate these two concepts. The most important is that firms can be innovative regardless of their stratum of technological intensity, which shows the importance of other capabilities to ensure the innovation’s success.