bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive shear stress analysis of turbulent boundary layer profiles
Authors: Womack Kristofer M. , Meneveau Charles , Schultz Michael P. 
Published: September 2019

Motivated by the need for accurate determination of wall shear stress from profile measurements in turbulent boundary layer flows, the total shear stress balance is analysed and reformulated using several well-established semi-empirical relations. The analysis highlights the significant effect that small pressure gradients can have on parameters deduced from data even in nominally zero pressure gradient boundary layers. Using the comprehensive shear stress balance together with the log-law equation, it is shown that friction velocity, roughness length and zero-plane displacement can be determined with only velocity and turbulent shear stress profile measurements at a single streamwise location for nominally zero pressure gradient turbulent boundary layers. Application of the proposed analysis to turbulent smooth- and rough-wall experimental data shows that the friction velocity is determined with accuracy comparable to force balances (approximately 1 %–4 %). Additionally, application to boundary layer data from previous studies provides clear evidence that the often cited discrepancy between directly measured friction velocities (e.g. using force balances) and those derived from traditional total shear stress methods is likely due to the small favourable pressure gradient imposed by a fixed cross-section facility. The proposed comprehensive shear stress analysis can account for these small pressure gradients and allows more accurate boundary layer wall shear stress or friction velocity determination using commonly available mean velocity and shear stress profile data from a single streamwise location.
Identifying and mapping very small (<0.5 km2) mountain glaciers on coarse to high-resolution imagery
Authors: Leigh J. R. , Stokes C. R. , Carr R. J. , Evans I. S. , Andreassen L. M. , Evans D. J. A. 
Published: September 2019

AbstractSmall mountain glaciers are an important part of the cryosphere and tend to respond rapidly to climate warming. Historically, mapping very small glaciers (generally considered to be <0.5 km2) using satellite imagery has often been subjective due to the difficulty in differentiating them from perennial snowpatches. For this reason, most scientists implement minimum size-thresholds (typically 0.01–0.05 km2). Here, we compare the ability of different remote-sensing approaches to identify and map very small glaciers on imagery of varying spatial resolutions (30–0.25 m) and investigate how operator subjectivity influences the results. Based on this analysis, we support the use of a minimum size-threshold of 0.01 km2for imagery with coarse to medium spatial resolution (30–10 m). However, when mapping on high-resolution imagery (<1 m) with minimal seasonal snow cover, glaciers <0.05 km2and even <0.01 km2are readily identifiable and using a minimum threshold may be inappropriate. For these cases, we develop a set of criteria to enable the identification of very small glaciers and classify them ascertain,probableorpossible.This should facilitate a more consistent approach to identifying and mapping very small glaciers on high-resolution imagery, helping to produce more comprehensive and accurate glacier inventories.
Coexistence of three specialist predators of the hemlock woolly adelgid in the Pacific Northwest USA
Authors: Rose Alexander , Ross Darrell W. , Havill Nathan P. , Motley Kyle , Wallin Kimberly F. 
Published: September 2019

AbstractThe hemlock woolly adelgid (Hemiptera: Adelgidae: Adelges tsugae Annand) is an invasive insect, introduced from Japan to eastern North America, where it causes decline and death of hemlock trees. There is a closely related lineage of A. tsugae native to western North America. To inform classical biological control of A. tsugae in the eastern USA, the density and phenology of three native western adelgid specialist predators, Leucopis argenticollis (Zetterstedt), Le. piniperda (Malloch) (Diptera: Chamaemyiidae), and Laricobius nigrinus Fender (Coleoptera: Derodontidae), were quantified in the Pacific Northwest. Infested branches were collected from western hemlock (Pinaceae: Tsuga heterophylla (Raf.) Sarg.) at four sites around the Puget Sound, Washington and three sites in Oregon. Immature Leucopis were identified to species using DNA barcodes. Leucopis argenticollis was roughly twice as abundant as Le. piniperda. Laricobius nigrinus larvae were more abundant than the two species of Leucopis during the egg stage of the first adelgid generation, but Leucopis were present as feeding larvae during the second adelgid generation when La. nigrinus was aestivating in the soil, resulting in Leucopis being more abundant than La. nigrinus across the entire sampling period. Adelges tsugae and La. nigrinus densities were not correlated, while A. tsugae and Leucopis spp. densities were positively correlated. Leucopis spp. and La. nigrinus densities were negatively correlated. These results support the complementary use of La. nigrinus and the two Leucopis species for biological control of A. tsugae in the eastern USA, and point to the need for further investigation of spatial and temporal niche partitioning among the three predator species.
Analysis of the effects of nanosilver on bacterial community in the intestinal fluid of silkworms using high-throughput sequencing
Authors: Lin Chen , Wei Zhou , Yi Zhou , Tingting Tan , Huamao Du , Lichun Feng 
Published: September 2019

AbstractNanosilver is an environment-friendly, harmless alternative of traditional disinfectants which can be potentially applied in the sericulture industry. However, the effects of nanosilver on the intestinal bacterial community of the silkworms (Bombyx mori L.) are unclear. In this study, Illumina MiSeq high-throughput sequencing technology was used to assess the intestinal bacterial community in both male and female silkworms while treated with different concentrations of nanosilver. We found that nanosilver significantly influenced the composition of silkworm intestinal bacterial community on the different taxonomic levels. Most conspicuously, the abundance of Firmicutes was increased by the treatment of 20 mg L−1 nanosilver but decreased by that of 100 mg L−1 nanosilver at the phylum level. The same trend was observed in Bacilli at the class level and in Enterococcus at the genus level. In some extreme cases, application of nanosilver eliminated the bacterium, e.g., Brevibacillus, but increased the population of several other bacteria in the host intestine, such as Blautia, Terrisporobacter, Faecalibacterium, and some bacteria could only be found in nanosilver treatment groups, e.g., Dialister. In addition, although nanosilver generally showed negative effects on the cocooning rate in a dose-dependent manner, we found that 20 mg L−1 nanosilver treatment significantly increased the body weight of silkworms and did not show negative effects on the survival rate. These results indicated that the intestinal bacteria community of silkworm larvae was significantly changed after nanosilver treatment which might consequently influence host growth and development.
Induced eggplant resistance against Trialeurodes vaporariorum triggered by jasmonic acid, abscisic acid, and Nesidiocoris tenuis feeding
Authors: Esmaeily Saeideh , Amin Samih Mohammad , Izadi Hamzeh 
Published: September 2019

AbstractGreenhouse whitefly, Trialeurodes vaporariorum Westwood, is one of the major insect pests of agricultural crops such as eggplant. Due to various difficulties associated with synthetic pesticides, more environmentally friendly alternative methods are greatly appreciated for controlling pests. In the present study, the induction of resistance was investigated in eggplant using root and foliar application of jasmonic acid, abscisic acid, as well as Nesidiocoris tenuis (Reuter) either individually or in combination against T. vaporariorum. The experiments were carried out under laboratory conditions inside a growth chamber, which was set at 27 ± 2°C, 50 ± 5% relative humidity with a 16 h day length. Our results showed an increase in plant resistance due to the higher immature mortality rates, longer immature periods, lower longevity of adults, and fecundity. In free-choice situation, oviposition on root jasmonic acid (RJA) + N. tenuis and root abscisic acid (RABA) + N. tenuis was similar, but numbers of eggs deposited on these plants were lower than other treatments and control plants. The plant enzyme activity and phenolic content were significantly greater in RJA + N. tenuis and RABA + N. tenuis, intermediate in individual treatments, and the lowest in control plants. Correspondingly, T. vaporariorum longevity, number of eggs produced per female, oviposition preference, all were lowest when the insects fed on these treatments. These findings suggest that the induction of resistance in eggplants with the physiological changes in the host plant leads to a reduction in whitefly damage.
Emotions, finances and independence. Uranium as a “happy object” in the Greenlandic debate on secession from Denmark
Authors: Thisted Kirsten 
Published: September 2019

AbstractAnalysing the Danish-Greenlandic debate on Greenland’s plans to extract and export uranium, the article advocates bringing the fields of extraction studies and cultural studies into dialogue. Drawing on discourse analysis, critical theory and the “emotional turn” in social sciences, the article demonstrates how the current discussion about secession is linked to a Danish-Greenlandic affective economy instituted during the colonial era. Conceived as the antithesis to the unhappy condition of present postcoloniality, independence has become the ultimate political goal for the Greenlandic nation. The reasoning is that history has made the Greenlanders citizens in a foreign nation, which has left them in a state of alienation. In order to lock colonialism away firmly in the past and attain future happiness, the Greenlanders must attain statehood. Uranium is supposed to promote this goal and is thus circulated as a “happy object”, positioning opponents of uranium mining as “affect aliens” or “killjoys” in the independence discourse. In Denmark, the Greenlandic detachment has led to “postcolonial melancholia” – and to a greater receptiveness to the Greenland desire for equality. In Greenland, disappointed expectations of rapid economic progress and growing distrust of large-scale projects have sparked a discussion about the significations of the concept of “independence”.
The ice as leitmotif of a life: Erich von Drygalski, writings and photographs
Authors: Millar Pat 
Published: September 2019

AbstractProfessional scientist-geographer Erich von Drygalski led the first German expedition to Antarctica in 1901–1903. The expedition saw itself as purely scientific, which turned out to be at odds with the expectations of Imperial Germany at the time. It was one of the first to use photography extensively and effectively to document and record scientific activities and to shape the public’s image of the work that was being done in this remote and unknown part of the world. Ice was the leitmotif of Drygalski’s life. He had prior experience in the Arctic, and the year spent in Antarctica confirmed his nuanced way of viewing the ice: on the one hand, and foremost, scholarly and objective, while still appreciating its aesthetic qualities; on the other, infused with feelings of human vulnerability. Using discourse analysis, this article examines Drygalski’s published work and photographs he chose to illustrate it, in order to investigate what the ice meant to him. In his writings, it was the scholarly, objective attitude which predominated and this may have contributed to the generally lacklustre reception of his Antarctic achievements. The photographs he chose to illustrate his published work, however, were many and varied, often capturing the awe-inspiring beauty of the ice and contributing to good sales of his narrative of the South Polar Expedition.
What can artificial languages reveal about morphosyntactic processing in bilinguals?
Authors: Grey Sarah 
Published: September 2019

AbstractThis article reviews work that has employed artificial languages to investigate the learning and processing of additional language grammar in bilinguals, with a focus on morphosyntactic processing in sentence contexts. The article first discusses research that has utilized artificial languages to elucidate two central issues in research on bilingual third language learning and processing: the role of prior language-learning experience and cross-linguistic transfer from the native and second languages to the third. Then, research that has compared bilingual third language to monolingual second language grammar processing is discussed, with specific consideration of hypothesized bilingual advantages at language learning. Finally, future directions in artificial language learning research on bilingual morphosyntactic processing are considered.
Engaging with the Sustainable Development Goals by going beyond Modernity: An ethical evaluation within a worldview framework
Authors: de Vries Bert J. M. 
Published: September 2019

Non-technical summaryThe UN Sustainable Development Goals (SDGs) and targets are an important achievement. They largely reflect the worldview of Modernity, with its emphasis on scientific and planning rationality and emancipatory ideals. This worldview is no longer evident and dominant, and it is time to systematically explore complementing worldviews. Explicit use of worldviews, as sets of values and beliefs, and ethics enriches the interpretation and implementation of the SDGs. This facilitates the engagement of citizens in their real-world diversity and the development of respect for and empathy and cooperation with people holding other values, beliefs and morals. Dialogue around ‘the middle road’ can give guidance to just, fair and sustainable development pathways.
Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria)
Authors: Assié Adrien , Leisch Nikolaus , Meier Dimitri V , Gruber-Vodicka Harald , Tegetmeyer Halina E , Meyerdierks Anke , Kleiner Manuel , Hinzke Tjorven , Joye Samantha , Saxton Matthew , Dubilier Nicole , Petersen Jillian M 
Published: September 2019

Abstract
Most autotrophs use the Calvin–Benson–Bassham (CBB) cycle for carbon fixation. In contrast, all currently described autotrophs from the Campylobacterota (previously Epsilonproteobacteria) use the reductive tricarboxylic acid cycle (rTCA) instead. We discovered campylobacterotal epibionts (“Candidatus Thiobarba”) of deep-sea mussels that have acquired a complete CBB cycle and may have lost most key genes of the rTCA cycle. Intriguingly, the phylogenies of campylobacterotal CBB cycle genes suggest they were acquired in multiple transfers from Gammaproteobacteria closely related to sulfur-oxidizing endosymbionts associated with the mussels, as well as from Betaproteobacteria. We hypothesize that “Ca. Thiobarba” switched from the rTCA cycle to a fully functional CBB cycle during its evolution, by acquiring genes from multiple sources, including co-occurring symbionts. We also found key CBB cycle genes in free-living Campylobacterota, suggesting that the CBB cycle may be more widespread in this phylum than previously known. Metatranscriptomics and metaproteomics confirmed high expression of CBB cycle genes in mussel-associated “Ca. Thiobarba”. Direct stable isotope fingerprinting showed that “Ca. Thiobarba” has typical CBB signatures, suggesting that it uses this cycle for carbon fixation. Our discovery calls into question current assumptions about the distribution of carbon fixation pathways in microbial lineages, and the interpretation of stable isotope measurements in the environment.
Sourcing the immune system to induce immunogenic cell death in Kras-colorectal cancer cells
Authors: Cirone Mara , Lotti Lavinia Vittoria , Granato Marisa , Renzo Livia Di , Biunno Ida , Cattaneo Monica , Verginelli Fabio , Vespa Simone , Davies Derek , Wells Valerie , Mariani-Costantini Renato , Mallucci Livio 
Published: September 2019

Abstract

Background
Current approaches aimed at inducing immunogenic cell death (ICD) to incite an immune response against cancer neoantigens are based on the use of chemotherapeutics and other agents. Results are hampered by issues of efficacy, combinatorial approaches, dosing and toxicity. Here, we adopted a strategy based on the use of an immunomolecule that overcomes pharmachemical limitations.

Methods
Cytofluorometry, electron microscopy, RT-PCR, western blotting, apotome immunofluorescence, MLR and xenografts.

Results
We report that an ICD process can be activated without the use of pharmacological compounds. We show that in Kras-mut/TP53-mut colorectal cancer cells the 15 kDa βGBP cytokine, a T cell effector with onco-suppressor properties and a potential role in cancer immunosurveillance, induces key canonical events required for ICD induction. We document ER stress, autophagy that extends from cancer cells to the corresponding xenograft tumours, CRT cell surface shifting, ATP release and evidence of dendritic cell activation, a process required for priming cytotoxic T cells into a specific anticancer immunogenic response.

Conclusions
Our findings provide experimental evidence for a rationale to explore a strategy based on the use of an immunomolecule that as a single agent couples oncosuppression with the activation of procedures necessary for the induction of long term response to cancer.
Dynamin and reverse-mode sodium calcium exchanger blockade confers neuroprotection from diffuse axonal injury
Authors: Omelchenko Anton , Shrirao Anil B. , Bhattiprolu Atul K. , Zahn Jeffrey D. , Schloss Rene S. , Dickson Samantha , Meaney David F. , Boustany Nada N. , Yarmush Martin L. , Firestein Bonnie L. 
Published: September 2019

AbstractMild traumatic brain injury (mTBI) is a frequently overlooked public health concern that is difficult to diagnose and treat. Diffuse axonal injury (DAI) is a common mTBI neuropathology in which axonal shearing and stretching induces breakdown of the cytoskeleton, impaired axonal trafficking, axonal degeneration, and cognitive dysfunction. DAI is becoming recognized as a principal neuropathology of mTBI with supporting evidence from animal model, human pathology, and neuroimaging studies. As mitochondrial dysfunction and calcium overload are critical steps in secondary brain and axonal injury, we investigated changes in protein expression of potential targets following mTBI using an in vivo controlled cortical impact model. We show upregulated expression of sodium calcium exchanger1 (NCX1) in the hippocampus and cortex at distinct time points post-mTBI. Expression of dynamin-related protein1 (Drp1), a GTPase responsible for regulation of mitochondrial fission, also changes differently post-injury in the hippocampus and cortex. Using an in vitro model of DAI previously reported by our group, we tested whether pharmacological inhibition of NCX1 by SN-6 and of dynamin1, dynamin2, and Drp1 by dynasore mitigates secondary damage. Dynasore and SN-6 attenuate stretch injury-induced swelling of axonal varicosities and mitochondrial fragmentation. In addition, we show that dynasore, but not SN-6, protects against H2O2-induced damage in an organotypic oxidative stress model. As there is currently no standard treatment to mitigate cell damage induced by mTBI and DAI, this work highlights two potential therapeutic targets for treatment of DAI in multiple models of mTBI and DAI.
Kinase-independent inhibition of cyclophosphamide-induced pathways protects the ovarian reserve and prolongs fertility
Authors: Bellusci Giovanna , Mattiello Luca , Iannizzotto Valentina , Ciccone Sarah , Maiani Emiliano , Villani Valentina , Diederich Marc , Gonfloni Stefania 
Published: September 2019

AbstractPremature ovarian failure and infertility are adverse effects of cancer therapies. The mechanism underlying chemotherapy-mediated depletion of the ovarian reserve remains unclear. Here, we aim to identify the signaling pathways involved in the loss of the ovarian reserve to prevent the damaging effects of chemotherapy. We evaluated the effects of cyclophosphamide, one of the most damaging chemotherapeutic drugs, against follicle reserve. In vivo studies showed that the cyclophosphamide-induced loss of ovarian reserve occurred through a sequential mechanism. Cyclophosphamide exposure induced the activation of both DNAPK-γH2AX-checkpoint kinase 2 (CHK2)-p53/TAp63α isoform and protein kinase B (AKT)-forkhead box O3 (FOXO3a) signaling axes in the nucleus of oocytes. Concomitant administration of an allosteric ABL inhibitor and cyclophosphamide modulated both pathways while protecting the ovarian reserve from chemotherapy assaults. As a consequence, the fertility of the treated mice was prolonged. On the contrary, the administration of an allosteric ABL activator enhanced the lethal effects of cyclophosphamide while shortening mouse fertility. Therefore, kinase-independent inhibition may serve as an effective ovarian-protective strategy in women under chemotherapy.
Determinants of maximum cup depth in non-glaucoma and primary open-angle glaucoma subjects: a population-based study
Authors: Zhang Qing , Zhang Ye , Xin Chen , Mao Yingyan , Cao Kai , Jan Catherine , Guo Chunyu , Wang Ningli , Thomas Ravi 
Published: September 2019

AbstractBackground/objectivesTo study the associations of intraocular pressure (IOP) and retinal vessel diameters: central retinal arteriolar equivalent (CRAE) and central retinal venular equivalent (CRVE) with the maximum cup depth (MCD) in subjects with and without POAG.Subjects/methodsEligible subjects from the Handan Eye Study. All participants underwent physical and comprehensive eye examinations. Univariable and multivariable linear regression models assessed the association between MCD and other parameters.ResultsFour thousand one hundred and ninety-four eligible nonglaucoma and 40 POAG subjects were analyzed. On univariable analysis, deeper MCD was significantly associated with younger age, male gender, lower systolic blood pressure (BP), higher IOP, higher estimated cerebro-spinal fluid pressure (ECSFP), lower estimated trans-laminal cribrosa pressure difference (ETLCPD), longer axial length, narrower CRAE, narrower CRVE, larger disc area (DA) and a lower prevalence of hypertension and diabetes. On multivariable analysis, significant independent determinants of MCD were larger DA (P < 0.001; beta: 0.042;B: 0.20; 95% CI: 0.19, 0.22), younger age (P < 0.001; beta: −0.09;B: −0.002; 95% CI: −0.003, −0.001), higher IOP (P < 0.01; beta: 0.040;B: 0.003; 95% CI: 0.001, 0.005), and narrower CRAE (P < 0.001; beta: −0.06;B: −0.001; 95% CI: −0.001, −0.0003). On adding ECSFP and ETLCPD to the model, MCD was associated with IOP but not with estimated CSFP and TLCPD. A 1 μm decrease in CRAE or 1 mmHg increase of IOP was associated with a 1 μm increase of MCD (P < 0.001) and 3 μm increase of MCD respectively (P = 0.009).ConclusionsNarrow CRVE and higher IOP are associated with an increase in MCD.