bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between thrombocytopenia and adverse outcomes in patients with atrial fibrillation: a systematic review and meta-analysis
Authors: Ding Qiuhua , Xu Wenlin , Chen Yaoyao , Chang Sijie , Zhang Jinhua 
Published: December 2024

BackgroundThrombocytopenia is often associated with adverse outcomes in patients with atrial fibrillation. Therefore, we conducted a meta-analysis to comprehensively assess the impact of thrombocytopenia on ischemic stroke/systemic embolism, major bleeding and all-cause mortality in patients with atrial fibrillation.MethodsTwo electronic databases, PubMed and Web of Science, were systematically searched from their inception to December 1, 2023, including the studies on the correlation between atrial fibrillation patients with thrombocytopenia and adverse outcomes. Relevant data was extracted, literature quality was evaluated, meta-analysis was performed by using REVMAN 5.4 software, and the results were reported with odds ratio (OR) of 95% confidence interval (CI).ResultsA total of 12 studies included 73,824 patients with atrial fibrillation (average age: 72.67, males: 42,275, 57.3%), among them, there were 7,673 patients combined with thrombocytopenia. The average follow-up time of these studies was 87 days to 55 months. Compared to no thrombocytopenia, atrial fibrillation patients combined with thrombocytopenia have a significant risk reduction of ischemic stroke/systemic embolism [OR: 0.79, 95% CI: (0.69, 0.91); P &lt; 0.01]. Nevertheless, the risk of both major bleeding [OR: 1.51, 95% CI: (1.20, 1.79), P &lt; 0.01] and all-cause mortality [OR: 1.40, 95% CI: (1.23, 1.61); P &lt; 0.01] is significantly higher in thrombocytopenia group.ConclusionsThrombocytopenia has an important impact on the prognosis of patients with atrial fibrillation. Thrombocytopenia is significantly associated with a lower risk of ischemic stroke/systemic embolism but a higher risk of major bleeding and all-cause mortality. Attention to thrombocytopenia and optimization of treatment may be the effective way to improve the prognosis of atrial fibrillation with thrombocytopenia.Systematic Review Registrationhttps://www.crd.york.ac.uk/, PROSPERO Registration Number: (CRD42023459916).
Case Report: A novel method of needle-free transseptal puncture
Authors: Lee Chia-Chen , Chao Chen-An , Chang Su-Huan , Chen Chun-Kai , Chen Yen-Siou , Lin Chang-En , Jeng Tsung-Ping , Yu Chih-Chieh 
Published: December 2024

BackgroundIn the era of fluoroless catheter ablation (CA), achieving a successful transseptal puncture (TSP) presents a significant challenge. We introduce a novel technique for zero-fluoroscopy and cost-effective needle-free TSP.Case summaryWe describe two cases where a GMS-1 guidewire (0.025 inch, pigtail configuration; Toray Medical Co., Ltd., Japan) was utilized for TSP. This technique was performed using either fluoroscopy or intracardiac echocardiography (ICE). The procedure was completed successfully in both cases, with no complications reported.ConclusionThe use of a 0.025 inch GMS-1 guidewire with an electrocautery technique enables effective transseptal puncture without the need for a needle or fluoroscopy. This novel approach offers a safe, efficient, and zero-fluoroscopic alternative for TSP.
Depression and anxiety among pregnant women during COVID 19 pandemic in Ethiopia: a systematic review and meta-analysis
Authors: Gebeyehu Wondmeneh Temesgen , Wogris Mohhamed 
Published: December 2024

BackgroundCoronavirus Disease-19 pandemic had an adverse impact on the mental health of the public worldwide, but the problem is worst among pregnant women due to social distancing policies and mandatory lockdown, including prenatal care services. As a result, the prevalence of depression and anxiety could increase during the pandemic, particularly among pregnant women. Thus, the purpose of this review is to determine the magnitude of depression and anxiety and contributing factors among pregnant women during the pandemic in Ethiopia.MethodsWeb of Science, Since Direct, PubMed, Google Scholar, and African Journals Online were the electronic databases searched, the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) reporting guidelines were followed in this review. The Newcastle-Ottawa Critical Appraisal Checklist was used to assess the quality of the included studies. A predefined data extraction sheet developed in Excel was used to extract the data. The pooled prevalence of anxiety and depression was determined by a random effect model meta-analysis.Results4,269 and 1,672 pregnant women were involved in depression and anxiety studies, respectively. The pooled prevalence of depression and anxiety among pregnant women during the COVID-19 pandemic in Ethiopia was 24.7% (95% CI: 18.52–30.87) and 35.19% (95% CI: 26.83–43.55), respectively. Single marital status (AOR = 2.22, 95% CI: 1.07–3.37), poor social support (AOR = 2.7, 95% CI: 1.06–4.35), unplanned pregnancies (AOR = 2.17, 95% CI: 1.34–3.0), and unsatisfied marital status (AOR = 2.16, 95% CI: 1.17–3.14) were risk factors for depression. Violence against intimate partners (AOR = 2.87, 95% CI: 1.97–3.77) and poor social support (AOR = 1.98, 95% CI: 1.24–2.71) were risk factors for anxiety.ConclusionOne-fourth and nearly one-third of pregnant women had depression and anxiety, respectively, during COVID-19 pandemic in Ethiopia. Single or unsatisfied marital status and unplanned pregnancies were risk factors for depression. Poor social support was significantly associated with depression and anxiety. Pregnant women who experienced violence against intimate partners had higher anxiety. After COVID-19 pandemic, mental health interventions are essential for reducing depression and anxiety.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=527148, PROSPERO (CRD42024527148).
Unique lymphocyte transcriptomic profiles in septic patients with chronic critical illness
Authors: Barrios Evan L. , Balzano-Nogueira Leandro , Polcz Valerie E. , Rodhouse Christine , Leary Jack R. , Darden Dijoia B. , Rincon Jaimar C. , Dirain Marvin L. , Ungaro Ricardo , Nacionales Dina C. , Larson Shawn D. , Sharma Ashish , Upchurch Gilburt , Wallet Shannon M. , Brusko Todd M. , Loftus Tyler J. , Mohr Alicia M. , Maile Robert , Bacher Rhonda , Cai Guoshuai , Kladde Michael P. , Mathews Clayton E. , Moldawer Lyle L. , Brusko Maigan A. , Efron Philip A. 
Published: December 2024

IntroductionDespite continued improvement in post-sepsis survival, long term morbidity and mortality remain high. Chronic critical illness (CCI), defined as persistent inflammation and organ injury requiring prolonged intensive care, is a harbinger of poor long-term outcomes in sepsis survivors. Current dogma states that sepsis survivors are immunosuppressed, particularly in CCI. Investigation of this immune suppression in heterogeneous immune populations across distinct clinical trajectories and outcomes, along with limited sampling access, is accessible via single-cell RNA sequencing (scRNA-seq).MethodsscRNA-seq analysis was performed on healthy subjects (n=12), acutely septic patients at day 4 ± 1 (n=4), and those defined as rapid recovery (n=4) or CCI (n=5) at day 14-21. Differential gene expression and pathway analyses were performed on peripheral blood lymphocytes at both a population and annotated cell subset level. Cellular function was assessed via enzyme-linked immunosorbent spot (ELISpot), cytokine production analysis, and T-cell proliferation assays on an additional cohort of septic patients (19 healthy, 68 acutely septic, 27 rapid recovery and 20 classified as CCI 14-21 days after sepsis onset).ResultsSepsis survivors that developed CCI exhibited proportional shifts within lymphoid cell populations, with expanded frequency of CD8+ and NK cells. Differential expression and pathway analyses revealed continued activation in T cells and NK cells, with generalized suppression of B-cell function. Both T and NK cell subsets displayed transcriptomic profiles of exhaustion and immunosuppression in CCI, particularly in CD8+ T effector memory (TEM) cells and NK cells. Functional validation of T-cell behavior in an independent cohort demonstrated T cells maintained proliferative responses in vitro yet exhibited a marked loss of cytokine production. IFN-γ production at the acute phase (day 4 ± 1) was significantly reduced in subjects later classified as CCI.DiscussionSepsis patients exhibit unique T-, B-, and NK-cell transcriptional patterns that are both time- and clinical trajectory-dependent. These transcriptomic and pathway differences in sepsis patients that develop CCI are associated with exhaustion in CD8+ TEM cells and NK cells. Understanding the specific immune system patterns of different cell subsets after sepsis at a molecular level will be key to the development of personalized immunotherapy and drug-targeting intervention.Clinical trial registrationhttps://clinicaltrials.gov/, identifier NCT02276417.
A new perspective on macrophage-targeted drug research: the potential of KDELR2 in bladder cancer immunotherapy
Authors: Zhao Zhiyi , Jia Hongling , Sun Zhou , Li Yumeng , Liu Lingyun 
Published: December 2024

IntroductionBladder cancer was recognized as one of the most common malignant tumors in the urinary system, and treatment options remained largely limited to conventional surgery, radiotherapy, and chemotherapy, which limited patient benefits.MethodsResearchers constructed an RNA transcriptome map of bladder cancer by integrating single-cell RNA sequencing and clinical data, identifying potential molecular targets for diagnosis and treatment. We also verified the antitumor activity of the target through in vitro experiment.ResultsA distinct tumor cell subpopulation characterized by elevated S100A8 expression exhibited high copy number variation, high stemness, and low differentiation. It interacted with myeloid cells via the MIF-(CD74+CD44) and MIF-(CD74+CXCR4) signaling pathways. This study underscored KDELR2’s role in promoting cell proliferation, invasion, and migration, providing new therapeutic insights. Prognostic analysis revealed that KDELR2 correlated with poor survival, higher immune scores, and increased macrophage infiltration.DiscussionThe findings suggested that patients with high KDELR2 expression might benefit from immune checkpoint therapy. KDELR2 was also shown to enhance bladder cancer cell proliferation, invasion, and migration, highlighting it as a promising target for macrophage-focused drug development.
Integrating omics data and machine learning techniques for precision detection of oral squamous cell carcinoma: evaluating single biomarkers
Authors: Sun Yilan , Cheng Guozhen , Wei Dongliang , Luo Jiacheng , Liu Jiannan 
Published: December 2024

IntroductionEarly detection of oral squamous cell carcinoma (OSCC) is critical for improving clinical outcomes. Precision diagnostics integrating metabolomics and machine learning offer promising non-invasive solutions for identifying tumor-derived biomarkers.MethodsWe analyzed a multicenter public dataset comprising 61 OSCC patients and 61 healthy controls. Plasma metabolomics data were processed to extract 29 numerical and 47 ratio features. The Extra Trees (ET) algorithm was applied for feature selection, and the TabPFN model was used for classification and prediction.ResultsThe model achieved an area under the curve (AUC) of 93% and an overall accuracy of 76.6% when using top-ranked individual biomarkers. Key metabolic features significantly differentiated OSCC patients from healthy controls, providing a detailed metabolic fingerprint of the disease.DiscussionOur findings demonstrate the utility of integrating omics data with advanced machine learning techniques to develop accurate, non-invasive diagnostic tools for OSCC. The study highlights actionable metabolic signatures that have potential applications in personalized therapeutics and early intervention strategies.
Development and validation of a prediction model for the prognosis of renal cell carcinoma with liver metastases: a population-based cohort study
Authors: Wang Fei , Wang Pan , Wang Xihao , Lu Hengming , Han Yuchun , Wang Lianqu , Li Zhihui 
Published: December 2024

BackgroundCurrent studies on the establishment of prognostic model for renal cell carcinoma (RCC) with liver metastases (LM) were scarce. This study aimed to develop nomograms to predict the prognosis of RCC with LM.MethodsPatients diagnosed with RCC between 2010 and 2021 from the Surveillance, Epidemiology, and End Results (SEER) database were selected. The eXtreme Gradient Boosting (XGBoost) and Random Forest (RF) machine learning algorithms were used to screen for the most influential factors affecting prognosis, and the Venn diagram method was employed for further refinement. Subsequently, a nomogram related to brain metastases was constructed. The performance of the nomograms was evaluated through receiver operating characteristics (ROC) curves, calibration plots, C-index, time-dependent C-index, and decision curve analysis (DCA). Kaplan–Meier (K-M) survival curves were used to provide additional verification of the clinical efficacy of the nomogram.ResultsThis research comprised 2,395 RCC patients with LM. The Venn diagram demonstrated that age, histological type, grade, AJCC T stage, AJCC N stage, surgery, chemotherapy, marital status, and lung metastasis were highly relevant variables to patients with LM. The AUC, C-index, calibration curves, and DCA curves showed excellent performance of the nomogram. Additionally, the prognostic nomogram accurately classified RCC with LM patients into low- and high-risk groups for mortality.ConclusionThis study developed a novel nomogram to predict the prognostic factors of RCC with LM, providing a valuable reference for making accurate clinical decisions.
Probiotic effects on skin health: comprehensive visual analysis and perspectives
Authors: Deng Kexin , Fan Xiaofei , Yuan Zhigen , Li Dian 
Published: December 2024

BackgroundBacteria play a crucial role in maintaining the health of human skin. Research has demonstrated that probiotics present notable benefits for extraintestinal organs. Despite the extensive research on the impact of probiotics on skin health, there is a notable absence of regulatory frameworks governing their external application, with no approval from the FDA for any probiotic products for external use. The aim of this study is to offer a thorough summary of the research status in the field since 2000 and project future trends.MethodThe Web of Science Core Collection and SCI-Expanded index were selected for an extensive search of studies concerning the role of probiotics in skin health since 2000. A total of 1,306 publications were identified. Employing a double-blind method, two subsets of literature were scrutinized and subsequently combined for analysis. Using CiteSpace, this research explored key aspects such as primary countries, institutions, authors, journals, trending topics, research frontiers, and emerging patterns in research related to application of probiotic for skin health.ResultThis article included 709 research papers. The number of published papers has shown a rapid increase. The United States had the highest number of research papers (128), and Canada had the highest intermediate centrality (0.23). The University of California System emerged as the most prolific institution. Huang, Chun-Ming has published the most articles, and his research is at the forefront among those prolific authors. Twelve clusters were identified, with cluster #0 skin microbiota, #3 mechanisms, and #8 antimicrobial being the most recent. As for the hot topic, “diversity,” “health,” “skin microbiome,” “oxidative stress,” “microbiota,” and “antioxidants” have been at the forefront of the current field. The overall research trend has shifted from clinical trials to mechanistic exploration and from oral treatments to external applications, with the research level moving from general categories to specific strains.ConclusionThis paper summarized and visualized academic achievements in the field of probiotic application for skin health using CiteSpace and VOSviewer, offering a systematic and comprehensive perspective, along with a longitudinal overview of this research field.
Wide-spread brain alterations early after the onset of Crohn’s disease in children in remission—a pilot study
Authors: Filip Pavel , Vojtíšek Lubomír , Jičínská Anna Marie , Valenta Zdeněk , Horák Ondřej , Hrunka Matěj , Mangia Silvia , Michaeli Shalom , Jabandžiev Petr 
Published: December 2024

BackgroundThe research on possible cerebral involvement in Crohn’s disease (CD) has been largely marginalized and failed to capitalize on recent developments in magnetic resonance imaging (MRI).ObjectiveThis cross-sectional pilot study searches for eventual macrostructural and microstructural brain affection in CD in remission and early after the disease onset.Methods14 paediatric CD patients and 14 healthy controls underwent structural, diffusion weighted imaging and quantitative relaxation metrics acquisition, both conventional free precession and adiabatic rotating frame transverse and longitudinal relaxation time constants as markers of myelination, iron content and cellular loss.ResultsWhile no inter-group differences in cortical thickness and relaxation metrics were found, lower mean diffusivity and higher intracellular volume fraction were detected in CD patients over vast cortical regions essential for the regulation of the autonomous nervous system, sensorimotor processing, cognition and behavior, pointing to wide-spread cytotoxic oedema in the absence of demyelination, iron deposition or atrophy.ConclusionAlthough still requiring further validation in longitudinal projects enrolling larger numbers of subjects, this study provides an indication of wide-spread cortical oedema in CD patients very early after the disease onset and sets possible directions for further research.
Expression and clinical significance of Numb and Notch-1 proteins between tissue of colon cancer and regional lymph node metastases
Authors: Ma Jingyou , Zhen Jinpeng , Yang Ningbao , Meng Changjuan , Lian Yanjun 
Published: December 2024

ObjectiveTo investigate the expression and clinical significance of Notch-1 and Numb protein in colon cancer tissues and regional lymph node metastases.MethodsImmunohistochemical method was used to detect the expression of Notch-1 protein and Numb protein in 110 cases of colon cancer tissues, along with tumor adjacent tissues and 56 cases of MLN tissues, and to analyze its role in colon cancer and MLN tissue.ResultsComparing colon cancer tissue or lymph node metastases with tumor adjacent tissue, the positive expression rate of Numb was significantly decreased, while the positive expression of Notch-1 was significantly increased in colon cancer tissue or lymph node metastases (both p&lt;0.05). The expression of Notch-1 and Numb was correlated with the lymph node metastasis, TNM stage, and degree of differentiation (p&lt;0.05). The expression between Numb and Notch-1 showed negative correlation in colon cancer tissues (r=−0.261, p&lt;0.05). There was no relationship between the expression of Numb and Notch-1 protein in colon cancer and metastatic lymph node tissue (p&gt;0.05).ConclusionNumb expression is decreased and Notch-1 expression is increased in colon cancer tissue and metastatic lymph node tissue, suggesting that the interaction between the two proteins may play a promote role in the development, invasion, and metastasis of colon cancer. There was no relationship between the expression of Numb and Notch-1 protein in colon cancer and metastatic lymph node tissue, suggesting that there is no obvious enhancement of the cancer cells; in the process of lymph node metastasis, the degree of malignant biological behavior remains relatively stable.
Prognostic significance of CNNM4 in ovarian cancer: a comprehensive bioinformatics analysis
Authors: Wang Yiya 
Published: December 2024

BackgroundOvarian cancer (OV) is a common malignancy in the female reproductive system, characterized by poor prognosis and high recurrence rates. The discovery of dependable molecular markers is crucial for improving the timeliness of detection, diagnosis, and treatment, ultimately aiming to lower fatality rates. CNNM4 (cyclin and CBS domain divalent metal cation transport mediator 4), a member of the CNNM (Cyclin M) family, binds to PRL (prolactin) to regulate magnesium homeostasis and influence tumor cell proliferation. Although CNNM4 is implicated in various cancers, its role in OV remains unclear.MethodsIn vitro experiments assessed CNNM4 expression and its impact on the proliferation and migration of OV cells. Comparisons of TCGA and GTEx data were used to identify correlations between clinical features and outcomes. The role of CNNM4 in OV was further explored through comprehensive bioinformatics analyses.ResultsElevated levels of CNNM4 expression were observed in OV cells and tissues, and were linked to a poor prognosis. CNNM4 could modulate the proliferation and migration of various OV cell lines, including IOSE-80, SKOV-3, and A2780. Through involvement in multiple signaling pathways, evidenced by GSVA and GSEA, CNNM4 was implicated in OV progression. CNNM4 positively regulated the infiltration level of Macrophages M2, T cells CD4 memory resting and NK cells resting, and had a negative regulation effect on NK cells activated and T cells gamma delta. Moreover, CNNM4 is related to drug sensitivity of OV. A prediction model based on CNNM4 expression and clinical symptoms was constructed to predict OV prognosis.ConclusionCNNM4 may affect the progression of OV and is associated with a poor prognosis. It has potential as a biomarker for predicting survival and as a target for therapeutic interventions in OV patients.