bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Soft Contact Piezo-Controlled Liquid Cell for Probing Polymer Films under Confinement using Synchrotron FTIR Microspectroscopy
Authors: Benbow Natalie L. , Webber Jessie L. , Pawliszak Piotr , Sebben Damien A. , Ho Tracey T. M. , Vongsvivut Jitraporn , Tobin Mark J. , Krasowska Marta , Beattie David A. 
Published: December 2018

AbstractSoft polymer films, such as polyelectrolyte multilayers (PEMs), are useful coatings in materials science. The properties of PEMs often rely on the degree of hydration, and therefore the study of these films in a hydrated state is critical to allow links to be drawn between their characteristics and performance in a particular application. In this work, we detail the development of a novel soft contact cell for studying hydrated PEMs (poly(sodium 4-styrenesulfonate)/poly(allylamine hydrochloride)) using FTIR microspectroscopy. FTIR spectroscopy can interrogate the nature of the polymer film and the hydration water contained therein. In addition to reporting spectra obtained for hydrated films confined at the solid-solid interface, we also report traditional ATR FTIR spectra of the multilayer. The spectra (microspectroscopy and ATR FTIR) reveal that the PEM film build-up proceeds as expected based on the layer-by-layer assembly methodology, with increasing signals from the polymer FTIR peaks with increasing bilayer number. In addition, the spectra obtained using the soft contact cell indicate that the PEM film hydration water has an environment/degree of hydrogen bonding that is affected by the chemistry of the multilayer polymers, based on differences in the spectra obtained for the hydration water within the film compared to that of bulk electrolyte.
The formation of chromatin domains involves a primary step based on the 3-D structure of DNA
Authors: Bernardi Giorgio 
Published: December 2018

AbstractThe general model presented here for the formation of chromatin domains, LADs and TADs, is primarily based on the 3-D structures of the corresponding DNA sequences, the GC-poor and GC-rich isochores. Indeed, the low-heterogeneity GC-poor isochores locally are intrinsically stiff and curved because of the presence of interspersed oligo-Adenines. In contrast, the high-heterogeneity GC-rich isochores are in the shape of peaks characterized by increasing levels of GC and of interspersed oligo-Guanines. In LADs, oligo-Adenines induce local nucleosome depletions leading to structures that are well suited for the attachment to (and embedding in) the lamina. In TADs, the gradients of GC and of oligo-Guanines are responsible for a decreasing nucleosome density, decreasing supercoiling and increasing accessibility. This “moulding step” shapes the “primary TADs” into loops that lack self-interactions, being CTCF/cohesin-free structures. The cohesin complex then binds to the tips of “primary TADs” and slides down the loops, thanks to Nipbl, an essential factor for loading cohesin and for stimulating its ATPase activity and its translocation. This “extruding step” leads to closer contacts and to self-interactions in the loops and stops at the CTCF binding sites located at the base of the loops that are thus closed and insulated.
Fresh-blood-free diet for rearing malaria mosquito vectors
Authors: Marques Joana , Cardoso João C. R. , Felix Rute C. , Santana Rosa A. G. , Guerra Maria das Graças Barbosa , Power Deborah , Silveira Henrique 
Published: December 2018

AbstractMosquito breeding depends on the supply of fresh vertebrate blood, a major bottleneck for large-scale production of Anopheles spp. Feeding alternatives to fresh blood are thus a priority for research, outdoor large-cage trials and control interventions. Several artificial meal compositions were tested and Anopheles oogenesis, egg laying and development into the next generation of adult mosquitoes were followed. We identified blood-substitute-diets that supported ovarian development, egg maturation and fertility as well as, low progeny larval mortality, and normal development of offspring into adult mosquitoes. The formulated diet is an effective artificial meal, free of fresh blood that mimics a vertebrate blood meal and represents an important advance for the sustainability of Anopheles mosquito rearing in captivity.
An automated bacterial concentration and recovery system for pre-enrichment required in rapid Escherichia coli detection
Authors: Zhang Yushan , Xu Chang-qing , Guo Tianyi , Hong Lingcheng 
Published: December 2018

AbstractOne of the biggest challenges in rapid low concentration bacterial detection is the pre-concentration or pre-enrichment, which aims to increase bacteria concentration and reduce sample volume for easy bacterial detection. In practical bacterial detection, large-volume water samples with a pathogenic bacterial concentration of less than 1 CFU/mL have to be tested rapidly. The reported biosensors either have insufficient detection limit or have limited capability of handling a sufficiently large water sample. Therefore, a high-performance automated pre-enrichment process is strongly demanded in rapid practical bacterial detection. In this paper, a practical high performance automated bacterial concentration and recovery system (ABCRS) based on the combination of a ceramic membrane and tangential flow filtration technique was presented with short processing time (less than one hour), low pre-enrichment limit (≤0.005 CFU/mL), high concentration ratio (≥ 500), high recovery efficiency (~ 90%), and small final retentate volume (≤ 5 mL).
Quantitative Model for Ion Transport and Cytoplasm Conductivity of Chinese Hamster Ovary Cells
Authors: Fazelkhah Azita , Braasch Katrin , Afshar Samaneh , Salimi Elham , Butler Michael , Bridges Greg , Thomson Douglas 
Published: December 2018

AbstractIn mammalian cells cytoplasm ion concentrations and hence cytoplasm conductivity is an important indicator of their physiological state. Changes in the cytoplasm conductivity has been associated with physiological changes such as progression of cancer and apoptosis. In this work, a model that predicts the effects of physiological changes in ion transport on the cytoplasm conductivity of Chinese hamster ovary (CHO) cells is demonstrated. We determined CHO-specific model parameters, Na+/K+ATPase pumps and ion channels densities, using a flux assay approach. The obtained sodium (PNa), potassium (PK) and chloride (PCl) permeability and Na+/K+ATPase pump density were estimated to be 5.6 × 10−8 cm/s, 5.6 × 10−8 cm/s, 3.2 × 10−7 cm/s and 2.56 × 10−11 mol/cm2, respectively. The model was tested by comparing the model predictions with the experimentally determined temporal changes in the cytoplasm conductivity of Na+/K+ATPase pump inhibited CHO cells. Cells’ Na+/K+ATPase pumps were inhibited using 5 mM Ouabain and the temporal behavior of their cytoplasm conductivity was measured using dielectrophoresis cytometry. The measured results are in close agreement with the model-calculated values. This model will provide insight on the effects of processes such as apoptosis or external media ion concentration on the cytoplasm conductivity of mammalian cells.
Susceptible and mCry3A resistant corn rootworm larvae killed by a non-hemolytic Bacillus thuringiensis Cyt1Aa mutant
Authors: Bravo Alejandra , López-Diaz Jazmin A. , Yamamoto Takashi , Harding Kathleen , Zhao Jian-Zhou , Mendoza Gretel , Onofre Janette , Torres-Quintero Mary-Carmen , Nelson Mark E. , Wu Gusui , Sethi Amit , Soberón Mario 
Published: December 2018

AbstractThe western corn rootworm (WCR)Diabrotica virgifera virgiferacauses substantial damage in corn. Genetically modified (GM) plants expressing someBacillus thuringiensis(Bt) insecticidal Cry proteins efficiently controlled this pest. However, changes in WCR susceptibility to these Bt traits have evolved and identification of insecticidal proteins with different modes of action against WCR is necessary. We show here for the first time that Cyt1Aa from Bt exhibits toxicity against WCR besides to the dipteranAedes aegyptilarvae. Cyt1Aa is a pore-forming toxin that shows no cross-resistance with mosquitocidal Cry toxins. We characterized different mutations in helix α-A from Cyt1Aa. Two mutants (A61C and A59C) exhibited reduced or absent hemolytic activity but retained toxicity toA.aegyptilarvae, suggesting that insecticidal and hemolytic activities of Cyt1Aa are independent activities. These mutants were still able to form oligomers in synthetic lipid vesicles and to synergize Cry11Aa toxicity. Remarkably, mutant A61C showed a five-fold increase insecticidal activity against mosquito and almost 11-fold higher activity against WCR. Cyt1Aa A61C mutant was as potent in killing WCR that were selected for resistance to mCry3A as it was against unselected WCR indicating that this toxin could be a useful resistance management option in the control of WCR.
Experimental severe malaria is resolved by targeting newly-identified monocyte subsets using immune-modifying particles combined with artesunate
Authors: Niewold Paula , Cohen Amy , van Vreden Caryn , Getts Daniel R. , Grau Georges E. , King Nicholas J. C. 
Published: December 2018

AbstractCurrent treatment of severe malaria and associated cerebral malaria (CM) and respiratory distress syndromes are directed primarily at the parasite. Targeting the parasite has only partial efficacy in advanced infection, as neurological damage and respiratory distress are due to accumulation of host blood cells in the brain microvasculature and lung interstitium. Here, computational analysis identifies Ly6Clomonocytes as a major component of the immune infiltrate in both organs in a preclinical mouse model. Specifically targeting Ly6Clomonocyte precursors, identified by adoptive transfer, with immune-modifying particles (IMP) prevents experimental CM (ECM) in 50% ofPlasmodium bergheiANKA-infected mice in early treatment protocols. Furthermore, treatment at onset of clinical ECM with 2 doses of a novel combination of IMP and anti-malarial drug artesunate results in 88% survival. This combination confers protection against ECM and mortality in late stage severe experimental malaria and provides a viable advance on current treatment regimens.
Magnetospheric access for solar protons during the January 2005 SEP event
Authors: Kalegaev Vladimir V. , Vlasova Natalia A. , Nazarkov Ilya S. , Melkova Sophia A. 
Published: December 2018

The early phase of the extraordinary solar energetic particle 20 January, 2005 event having the highest peak flux of any SEP in the past 50 years of protons with energies > 100 MeV is studied. Solar energetic particles (>16 MeV) entry to the Earth’s magnetosphere on January 20, 2005 under northward interplanetary magnetic field conditions is considered based on multi-satellite data analysis and magnetic field simulation. Solar wind parameters and interplanetary magnetic field data, as well as calculations in terms of the A2000 magnetospheric magnetic field model were used to specify conditions in the Earth’s environment corresponding to solar proton event. It was shown that during the early phase of the event energetic particle penetration into the magnetosphere took place in the regions on the magnetopause where the magnetospheric and interplanetary magnetic field vectors are parallel. Complex analysis of the experimental data on particle fluxes in the interplanetary medium (data from ACE spacecraft) and on low-altitude (POES) and geosynchronous (GOES) orbits inside the Earth’s magnetosphere show two regions on the magnetopause responsible for particle access to the magnetosphere: the near equatorial day-side region and open field lines window at the high-latitude magnetospheric boundary. Calculations in terms of A2000 magnetospheric magnetic field model and comparison with SuperDARN images support the link between high-latitude solar energetic particle precipitations and the region at the magnetopause where the magnetospheric field is coupled with northward IMF, allowing solar particles entrance into the magnetosphere and access to the northern polar cap.
Circulating Tissue Inhibitor of Metalloproteinase-4 levels are not a Predictor of Preeclampsia in the period between 20 and 25 Weeks of Gestation
Authors: Sandrim Valeria Cristina , Machado Jackeline , Bettiol Heloisa , Barbieri Marco Antonio , Cardoso Viviane Cunha , Palei Ana Carolina , Cavalli Ricardo Carvalho 
Published: December 2018

Abstract
Objective To evaluate whether the circulating level of tissue inhibitor of metalloproteinase-4 (TIMP-4) in the period between 20 and 25 weeks of gestation is a predictor of preeclampsia.
Methods We have performed a case-control study, nested in a prospective study cohort in Ribeirão Preto, in the state of São Paulo, Brazil. Of the 1,400 pregnant women evaluated between 20 and 25 weeks of gestation, 460 delivered in hospitals outside of our institution. Of the 940 pregnant women who completed the protocol, 30 developed preeclampsia. Healthy pregnant women (controls, n = 90) were randomly selected from the remaining 910 participants. From blood samples collected between 20 and 25 weeks of gestation, we performed a screening of 55 angiogenesis-related proteins in 4 cases and 4 controls. The protein TIMP-4 was the most differentially expressed between cases and controls. Therefore, we measured this protein in all cases (n = 30) and controls selected (n = 90).
Results There were no differences in the plasma TIMP-4 levels of cases compared with controls (1,144 ± 263 versus 1,160 ± 362 pg/mL, respectively; p > 0.05).
Conclusion Plasma TIMP-4 levels were not altered at 20 to 25 weeks of gestation, before the manifestation of clinical symptoms; therefore, they are not good predictors of the development of preeclampsia.
Malignant Vascular Tumors of the Liver in Adults
Authors: Rimola Jordi , Alves Venancio 
Published: December 2018

AbstractHepatic angiosarcoma and epithelioid hemangioendothelioma (EHE) might be clinically considered a spectrum since, although more frequently presenting indolent behavior, EHE occasionally evolves to high-grade neoplasms. However, in most circumstances, pathological and immunohistochemical patterns define this differential diagnosis. More recently, molecular pathways for angiosarcoma and for EHE from other organs and from soft tissue have been proved different, paving the way for future morpho-molecular assessment of their hepatic counterpart. The frequency of liver involvement by Kaposi sarcoma in HIV-infected patients is lower nowadays. Histological findings and immunostaining for HHV-8 Ag are characteristic. Hepatic small vessel neoplasms have been recently recognized as important mimickers of angiosarcoma. The criteria for this differential diagnosis and the clinical behavior, up to now considered favorable, must be further studied.
Älter werden mit Konzept
Authors: Hug Josef 
Published: December 2018

ZusammenfassungDass man dem Personalmangel in der Pflege etwas entgegensetzen kann, macht das
Klinikum Karlsruhe vor. Pflegedirektor Josef Hug beschreibt, wie sich das
Projekt „Lebensphasengerechtes Arbeiten“ umsetzen lässt.
Recent origin and evolution of obesity-income correlation across the United States
Authors: Bentley R. Alexander , Ormerod Paul , Ruck Damian J. 
Published: December 2018

AbstractFrom a gene-culture evolutionary perspective, the recent rise in obesity rates around the Developed world is unprecedented; perhaps the most rapid population-scale shift in human phenotype ever to occur. Focusing on the recent rise of obesity and diabetes in the United States, we consider the predictions of human behavioral ecology (HBE) versus the predictions of social learning (SL) of obesity through cultural traditions and/or peer–to–peer influence. To isolate differences that might discriminate these different models, we first explore temporal and geographic trends in the inverse correlation between household income and obesity and diabetes rates in the U.S. Whereas by 2015 these inverse correlations were strong, these correlations were non-existent as recently as 1990. The inverse correlations have evolved steadily over recent decades, and we present equations for their time evolution since 1990. We then explore evidence for a “social multiplier” effect at county scale over a ten-year period, as well as a social diffusion pattern at state scale over a 26–year period. We conclude that these patterns support HBE and SL as factors driving obesity, with HBE explaining ultimate causation. As a specific “ecological” driver for this human behavior, we speculate that refined sugar in processed foods may be a prime driver of increasing obesity and diabetes.
Review of agriculture-nutrition linkages in South Asia.
Authors: Bhavani R. V. , Rampal Priya 
Published: December 2018

Abstract
The paper reviews the evidence of agriculture-nutrition linkages with particular reference to South Asia from studies published during the period 2012-2018. South Asia houses the largest population of undernourished people in the world and a majority of the population in the region is dependent on agriculture and allied activities and live in rural areas. Following a review of agriculture nutrition linkage, the paper reviews recent work in South Asia focusing on the pathway of agriculture as an asset and source of food and the role of women in agriculture. The evidence from reviews of agriculture nutrition linkage finds lack of robust evidence of impact on nutrition outcomes. The studies from South Asia highlight the importance of production diversity, agriculture and land productivity and enabling factors of WASH and women's empowerment, for impact on dietary diversity and nutrition outcomes. This points to need for policy focus in these areas. Most of the studies were based on secondary data and the search yielded very few intervention-based studies; there were also no studies from Pakistan. The importance of leveraging agriculture for nutrition in order to progress towards the Sustainable Development Goals (SDGs) is now well accepted. Along with efforts to improve dietary diversity through agriculture, long-term intervention studies with robust design targeting nutrition indicators can help better inform policy.
Setting Neuro-Fuzzy PID Control In Plant Nonlinear Active Suspension
Authors: Andrew Ferdinandus Aprildy Randy , Nugraha Anggara Trisna , Jamaaluddin Jamaaluddin 
Published: December 2018

Abstract
The main problem in vibration in the vehicle is caused by the road surface. For that reason it is necessary to regulate the vibration of the vehicle suspension directly related to the road surface. Neuro-fuzzy PID controller is used to set the active suspension of the vehicle. The suspension model used is the active suspension quarter-car with nonlinear model. Faulty road surfaces are used as a disturbance in the system, interference changes in amplitude (height / depth) in the range of -25cm to 25cm. The simulation results show the performance of the PID neuro-fuzzy setting method that can overcome 77% of the amount of road noise.
A development process of enterprise applications with microservices
Authors: Vera-Rivera F H 
Published: December 2018

Abstract
This paper presents a development process of enterprises applications with microservices. We identify the construction phases, practices, fundamentals, methods and tools that are being used in the development of microservices based enterprise applications. After identifying and proposing the development process, it is validated and tested in the construction of an application called Sinplafut. By using microservices the size of enterprise applications is reduced and easier to manage, deployment, scaling, test and replace using the resources offered by cloud computing. In this way the costs and efforts necessary to maintain the application in the cloud are considerably reduced.
Technological innovation of the footwear sector companies in Cucuta and its metropolitan area
Authors: Romero Y A , Monroy R , Gelves J F 
Published: December 2018

Abstract
The objective of this research was to analyze the technological innovation of footwear companies in Cúcuta and its metropolitan area. A sample of 32 companies that are currently working (legal character) was selected. For the analysis of the technological innovation of the footwear sector companies, an information gathering instrument was applied where they were asked about the different organizational aspects, their technological innovation capabilities and innovation processes. In the dimension of innovation capacity, the level of human talent training, the orientation of human talent towards innovation, the financial liquidity for investment of R & D projects and the activities of collaboration and interaction with other organizations were addressed. In the innovation processes, the companies evaluated the development of new products, participation in technological innovation projects in process, participation in projects of technological innovation in product and existence of a Department of R+D+i. In the analysis, it was identified that companies in the footwear sector must strengthen their skills in human talent training and implement a culture of innovation within the organization that allows them to strengthen their innovation.
Nicotine Effects on White Matter Microstructure in Young Adults
Authors: Kangiser Megan M , Thomas Alicia M , Kaiver Christine M , Lisdahl Krista M 
Published: December 2018

Abstract

Objective
Nicotine use is widely prevalent among youth, and is associated with white matter microstructural changes as measured by diffusion tensor imaging (DTI). In adults, nicotine use is generally associated with lower fractional anisotropy (FA), but in adolescents/young adults (≤30 years), microstructure appears healthier, indicated by higher FA. This cross-sectional study examined associations between nicotine use and white matter microstructure using fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), and radial diffusivity (RD) in young adults.

Methods
Fifty-three participants (18 nicotine users [10 female]/35 controls [17 female]) ages 18–25 underwent MRI scan, neuropsychological battery, toxicology screening, and drug use interview. Nicotine group associations with FA and MD were examined in various white matter tracts. In significant tracts, AD and RD were measured. Exploratory correlations were conducted between significant tracts and verbal memory and sustained attention/working memory performance.

Results
Nicotine users exhibited significantly lower FA than controls in the left anterior thalamic radiation, left inferior longitudinal fasciculus, left superior longitudinal fasciculus—temporal, and left uncinate fasciculus. In these tracts, AD and RD did not differ, nor did MD differ in any tract. White matter quality was positively correlated with sustained attention/working memory performance.

Conclusions
Cigarette smoking may disrupt white matter microstructure. These results are consistent with adult studies, but inconsistent with adolescent/young adult studies, likely due to methodological and sample age differences. Further studies should examine longitudinal effects of nicotine use on white matter microstructure in a larger sample.