bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wall shear stress from jetting cavitation bubbles
Authors: Zeng Qingyun , Gonzalez-Avila Silvestre Roberto , Dijkink Rory , Koukouvinis Phoevos , Gavaises Manolis , Ohl Claus-Dieter 
Published: May 2018

The collapse of a cavitation bubble near a rigid boundary induces a high-speed transient jet accelerating liquid onto the boundary. The shear flow produced by this event has many applications, examples of which are surface cleaning, cell membrane poration and enhanced cooling. Yet the magnitude and spatio-temporal distribution of the wall shear stress are not well understood, neither experimentally nor by simulations. Here we solve the flow in the boundary layer using an axisymmetric compressible volume-of-fluid solver from the OpenFOAM framework and discuss the resulting wall shear stress generated for a non-dimensional distance, $\unicode[STIX]{x1D6FE}=1.0$ ($\unicode[STIX]{x1D6FE}=h/R_{max}$, where $h$ is the distance of the initial bubble centre to the boundary, and $R_{max}$ is the maximum spherical equivalent radius of the bubble). The calculation of the wall shear stress is found to be reliable once the flow region with constant shear rate in the boundary layer is determined. Very high wall shear stresses of 100 kPa are found during the early spreading of the jet, followed by complex flows composed of annular stagnation rings and secondary vortices. Although the simulated bubble dynamics agrees very well with experiments, we obtain only qualitative agreement with experiments due to inherent experimental challenges.
Self-organized oscillations of Leidenfrost drops
Authors: Ma Xiaolei , Burton Justin C. 
Published: May 2018

In the Leidenfrost effect, a thin layer of evaporated vapour forms between a liquid and a hot solid. The complex interactions between the solid, liquid and vapour phases can lead to rich dynamics even in a single Leidenfrost drop. Here we investigate the self-organized oscillations of Leidenfrost drops that are excited by a constant flow of evaporated vapour beneath the drop. We show that for small Leidenfrost drops, the frequency of a recently reported ‘breathing mode’ (Caswell, Phys. Rev. E, vol. 90, 2014, 013014) can be explained by a simple balance of gravitational and surface tension forces. For large Leidenfrost drops, azimuthal star-shaped oscillations are observed. Our previous work showed how the coupling between the rapid evaporated vapour flow and the vapour–liquid interface excites the star-shaped oscillations (Ma et al., Phys. Rev. Fluids, vol. 2, 2017, 031602). In our experiments, star-shaped oscillation modes of $n=2{-}13$ are observed in different liquids, and the number of observed modes depends sensitively on the viscosity of the liquid. Here we expand on this work by directly comparing the oscillations with theoretical predictions, as well as show how the oscillations are initiated by a parametric forcing mechanism through pressure oscillations in the vapour layer. The pressure oscillations are driven by the capillary waves of a characteristic wavelength beneath the drop. These capillary waves can be generated by a large shear stress at the liquid–vapour interface due to the rapid flow of evaporated vapour. We also explore potential effects of thermal convection in the liquid. Although the measured Rayleigh number is significantly larger than the critical Rayleigh number, the frequency (wavelength) of the oscillations depends only on the capillary length of the liquid, and is independent of the drop radius and substrate temperature. Thus convection seems to play a minor role in Leidenfrost drop oscillations, which are mostly hydrodynamic in origin.
Effect of biotite on the firing characteristics of certain weathered schists
Authors: Norrish K. , Radoslovich E. W. 
Published: May 2018

AbstractA study was made of five clays used for brickmaking to determine the reason why certain clays crumbled on firing. Partially weathered biotite was found to be a constituent of the clays which crumbled and the crumbling was due to this mineral exfoliating and so disrupting the bricks at a comparatively low temperature. Fresh biotite and highly weathered biotite do not have this adverse effect.
Scanning Transmission Electron Microscopy for Critical Dimension Monitoring in Wafer Manufacturing
Authors: Wang Haifeng , Fang Jason , Arjavac Jason , Kellner Rudy 
Published: May 2018

Abstract
Automated scanning transmission electron microscopy (STEM) metrology provides critical dimension (CD) measurements an order of magnitude more precise than comparable scanning electron microscopy (SEM) measurements. New developments in automation now also provide throughput and response time sufficient to support high volume microelectronic manufacturing processes. The newly developed methodology includes automated, focused ion beam (FIB) based sample preparation; innovative, ex-situ sample extraction; and automated metrology. Although originally developed to control the production of thin film magnetic heads for data storage, the technique is fully applicable to any wafer-based manufacturing process.
Passive Mirror Imaging through a Solid-State Back-Scattered Electron Detector
Authors: Croccolo Fabrizio , Riccardi Claudia 
Published: May 2018

Abstract
The scanning electron microscope (SEM) is commonly used to obtain images of a wide variety of samples within a wide range of magnification factors from the order of 10 up to about 105×. This technique is usually applied, but not limited to, the investigation of conductive samples. This is because the interaction of the scanning beam with the sample generates a net charge on the sample surface. Thus, if the sample is conductive, the charge can be quickly disposed of to ground, away from the beam spot. If the sample in non-conductive, the sample becomes locally charged, giving rise to a distortion of the primary beam. In certain conditions, the charge stored on the sample is able to reflect back the incoming electrons, much like an electrostatic mirror.
Beyond the Black Box: Interactive Global Docking of Protein Complexes
Authors: Heyd Jochen , Birmanns Stefan 
Published: May 2018

Abstract
A key to understanding the function of biological systems is the visualization of their natural state, ideally in a natural environment. At a molecular level, this is challenging. Traditional experimental techniques, like X-ray crystallography, can provide the atomic structure of proteins, but only by removing them from their native surroundings and forcing them into crystals. Over the past decade, microscopy techniques have emerged as alternatives to these traditional structure determination methods, with the advantage of visualizing molecules in a near-native state. Given the current focus of structural biology on interactions between proteins and better understanding of large protein complexes, cryo-electron microscopy (cryo-EM) has become a valuable tool.
The Use of Poly-L-lysine as an Adhesive in Scanning Electron Microscopy
Authors: Taylor Jeannette 
Published: May 2018

Abstract
Poly-L-lysine of a medium molecular weight, 30,000 to 70,000 Daltons (Sigma–Aldrich), is currently used in our facility as a polycationic adhesive on 5 mm square silicon chips to secure individual anionic cells and particles for ease of processing and viewing in the scanning electron microscope. In this technique, a solution of 1 mg/ml of poly-L-lysine is dissolved in distilled water. Drops of the solution are placed onto clean 5 mm square silicon chips and allowed to sit for one to several hours before being wicked away. The prepared chips are used immediately. Suspended cells are then applied to the chips and allowed to settle and adhere, after which they are washed and fixed. Alternatively, fixed suspended cells or particles are applied to these silicon chips. The cells and particles secured to the silicon chips are easily handled through processing steps such as dehydration, critical point drying, and metal sputter coating for viewing in the scanning electron microscope. Presented herein is a brief history of the evolution of the technique of using poly-L-lysine as an adhesive for SEM.
Multiscale processes in the genesis of a near‐equatorial tropical cyclone during the Dynamics of the MJO Experiment: Results from partial lateral forcing experiments
Authors: Yang Hongwei , Wang Bin 
Published: May 2018

AbstractTropical cyclone (TC) 05A (TC05A, November 2011) over the Indian Ocean originated near the equator and brought huge damages to Sri Lanka and India. The genesis and propagation of TC05A involves interactions with the Madden–Julian Oscillation (MJO), interannual (IA) variations, and other synoptic (SY) systems. To investigate the impacts of the SY, MJO, and IA forcings across the lateral boundaries on TC05A, regional simulation with the Weather Research and Forecast model through the partial lateral forcing approach is conducted. The control experiment reproduces realistic genesis and movement of TC05A and associated heavy rainfall, as well as the mean states and MJO. Both the SY and IA forcings make a moderate contribution to the initiation of cyclonic vorticity and rainfall enhancement in the central Indian Ocean and moderate modification of the TC05A track. However, it is the MJO forcing that fundamentally reproduces a coherent moist, low‐level convergence, and a cyclonic environment that is favorable for the development of TC05A. The greatest impact of northern boundary intraseasonal (IS) forcing on the genesis location and path of TC05A is further identified, suggesting the importance of the interaction between the MJO and northeast winter monsoon. In contrast, the southern boundary IS forcing is unimportant and the western and eastern boundary IS forcings play a moderate role in slowing down TC05A and modifying its track, intensity, and rainfall. This study demonstrates that the partial lateral forcing method is an effective tool to identify the key factors controlling the extreme events through regional climate modeling.
Statistical Distributions of Lightning Peak Currents: Why Do They Appear to Be Lognormal?
Authors: Slyunyaev Nikolay N. , Mareev Evgeny A. , Rakov Vladimir A. , Golitsyn Georgy S. 
Published: May 2018

AbstractThe statistical distribution of peak currents in cloud‐to‐ground lightning strokes is analyzed and discussed both in the context of the existing experimental data and from a theoretical viewpoint. The distributions of peak currents directly measured at instrumented towers are compared to those of peak currents reported by the U.S. National Lightning Detection Network and peak currents measured in rocket‐triggered lightning experiments; all those appear to be lognormal. To explain the observed peak current distributions, a simple model based on the large‐scale electric field evolution prior to lightning flashes is developed. Based only on the most general assumptions concerning the probability of occurrence of lightning discharge, it is shown that the distribution of the pre‐first‐stroke large‐scale electric field is close to lognormal in a certain range. With the introduction of structure factors relating the leader or cloud electric potential to this field, semiempirical relationships between the potential and the peak current in negative first and subsequent strokes imply that the peak current and pre‐first‐stroke large‐scale electric field obey distributions of the same type. It is demonstrated that with a suitable choice of parameters, the model‐predicted distributions are close to those obtained from direct measurements, and statistical tests show that their agreement with the data is at least as good as that seen for lognormal approximations.
Airborne Remote Sensing of Wave Propagation in the Marginal Ice Zone
Authors: Sutherland Peter , Brozena John , Rogers W. Erick , Doble Martin , Wadhams Peter 
Published: May 2018

AbstractAirborne scanning lidar was used to measure the evolution of the surface wave field in the marginal ice zone (MIZ) during two separate wave events in the Beaufort Sea in October 2015. The lidar data consisted of a 2‐D field of surface elevation with horizontal resolutions between 17 and 33 cm, over a swath approximately 150‐220 m wide, centred on the ground track of the aircraft. Those data were used to compute directional wavenumber spectra of the surface wave field. Comparison with nearly collocated buoy data found the lidar and buoy measurements to be generally consistent. During the first event, waves traveling from open water into the ice were attenuated by the ice. The low spectral spreading and
spectral dependence of the attenuation was consistent with dissipative models that treat sea ice as a highly viscous fluid floating on a less viscous ocean. Upper‐ocean eddy viscosities calculated using that model were found to be significantly lower than those from previous work. The second event was in off‐ice winds and cold temperatures, allowing measurement of the wave fetch relation in ice‐forming conditions. The wave growth rate was found to be slightly higher than previous measurements under unstable atmospheric conditions without ice formation. Comparison with WAVEWATCH III model output highlighted the importance of accurate ice information and fine geographic computational resolution when making predictions near the ice edge. Finally, the very short scales over which the wave field was observed to evolve in the MIZ are discussed.
Light-driven transformable optical agent with adaptive functions for boosting cancer surgery outcomes
Authors: Qi Ji , Chen Chao , Zhang Xiaoyan , Hu Xianglong , Ji Shenglu , Kwok Ryan T. K. , Lam Jacky W. Y. , Ding Dan , Tang Ben Zhong 
Published: May 2018

AbstractFluorescence and photoacoustic imaging have different advantages in cancer diagnosis; however, combining effects in one agent normally requires a trade-off as the mechanisms interfere. Here, based on rational molecular design, we introduce a smart organic nanoparticle whose absorbed excitation energy can be photo-switched to the pathway of thermal deactivation for photoacoustic imaging, or to allow opposed routes for fluorescence imaging and photodynamic therapy. The molecule is made of a dithienylethene (DTE) core with two surrounding 2-(1-(4-(1,2,2-triphenylvinyl)phenyl)ethylidene)malononitrile (TPECM) units (DTE-TPECM). The photosensitive molecule changes from a ring-closed, for photoacoustic imaging, to a ring-opened state for fluorescence and photodynamic effects upon an external light trigger. The nanoparticles’ photoacoustic and fluorescence imaging properties demonstrate the advantage of the switch. The use of the nanoparticles improves the outcomes of in vivo cancer surgery using preoperative photoacoustic imaging and intraoperative fluorescent visualization/photodynamic therapy of residual tumours to ensure total tumour removal.
Screening for the anti-inflammation quality markers of Xiaojin Pills based on HPLC-MS/MS method, COX-2 inhibition test and protein interaction network
Authors: Xiong Xi , He Ya-nan , Feng Bi , Pan Yuan , Zhang Hai-zhu , Ke Xiu-mei , Zhang Yi , Yang Ming , Han Li , Zhang Ding-kun 
Published: May 2018

AbstractNowadays, breast disorders seriously affect women’s health in an increasing number. In China, Xiaojin Pills are commonly used in the treatment of breast diseases. Doctors have concluded that the combined use of Xiaojin Pills with conventional therapy can significantly improve the efficacy with fewer side effects. However, the prescription of Xiaojin Pills is complicated and their quality control methods cannot completely ensure the quality of Xiaojin Pills. On the basis of its mechanism, our study combined chemical evaluation and biological evaluation to identify the anti-inflammatory markers of Xiaojin Pills. In this manuscript, 13 compounds in Xiaojin Pills were quantified. At the same time, the cyclooxygenase-2 inhibition rates of different Xiaojin Pills were measured and the possible markers were screened by spectrum-effect relationship. Further, anti-inflammatory activities of markers were verified and protein interaction network was analyzed, identifying the components of Protocatechuate, Beta-Boswellic acid and Levistilide A as the anti-inflammatory quality markers of Xiaojin Pills. We hope our studies can provide a scientific theoretical basis for accurately quality control of Xiaojin Pills and reasonable suggestions for pharmaceutical companies and new ideas for the quality control of other medicines.
Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine
Authors: Fan Yi-Chin , Chen Jo-Mei , Lin Jen-Wei , Chen Yi-Ying , Wu Guan-Hong , Su Kuan-Hsuan , Chiou Ming-Tang , Wu Shang-Rung , Yin Ji-Hang , Liao Jiunn-Wang , Chang Gwong-Jen J. , Chiou Shyan-Song 
Published: May 2018

AbstractSwine are a critical amplifying host involved in human Japanese encephalitis (JE) outbreaks. Cross-genotypic immunogenicity and sterile protection are important for the current genotype III (GIII) virus-derived vaccines in swine, especially now that emerging genotype I (GI) JE virus (JEV) has replaced GIII virus as the dominant strain. Herein, we aimed to develop a system to generate GI JEV virus-like particles (VLPs) and evaluate the immunogenicity and protection of the GI vaccine candidate in mice and specific pathogen-free swine. A CHO-heparan sulfate-deficient (CHO-HS(-)) cell clone, named 51-10 clone, stably expressing GI-JEV VLP was selected and continually secreted GI VLPs without signs of cell fusion. 51-10 VLPs formed a homogeneously empty-particle morphology and exhibited similar antigenic activity as GI virus. GI VLP-immunized mice showed balanced cross-neutralizing antibody titers against GI to GIV viruses (50% focus-reduction micro-neutralization assay titers 71 to 240) as well as potent protection against GI or GIII virus infection. GI VLP-immunized swine challenged with GI or GIII viruses showed no fever, viremia, or viral RNA in tonsils, lymph nodes, and brains as compared with phosphate buffered saline-immunized swine. We thus conclude GI VLPs can provide sterile protection against GI and GIII viruses in swine.
Improvement of the activity of the anti-HIV-1 integrase aptamer T30175 by introducing a modified thymidine into the loops
Authors: Virgilio Antonella , Amato Teresa , Petraccone Luigi , Esposito Francesca , Grandi Nicole , Tramontano Enzo , Romero Raquel , Haider Shozeb , Gomez-Monterrey Isabel , Novellino Ettore , Mayol Luciano , Esposito Veronica , Galeone Aldo 
Published: May 2018

AbstractIn this paper, we report our investigations on analogues of the anti-human immunodeficiency virus type 1 (HIV-1) integrase (IN) aptamer T30175 in which the individual thymidines forming the loops were replaced by 5-hydroxymethyl-2′-deoxyuridine residues (H). Circular dichroism, nuclear magnetic resonance and gel electrophoresis investigations clearly indicated that all the modified aptamers preserve the ability to form the original 5′-5′ end-stacked head-to-head dimeric G-quadruplex structure, in which each G-quadruplex adopts a parallel arrangement and is characterized by three G-tetrads, three propeller loops and one bulge-loop. All the modified aptamers were tested in an IN inhibition LEDGF-independent assay. While the modified aptamers INTB-H13 and INTB-H17 showed IC50 values comparable with that of the parent aptamer (INTB-nat), analogues INTB-H2, INTB-H5 and, to a lesser extent, INTB-H9 showed a higher ability to inhibit the HIV IN than the unmodified aptamer. Molecular modelling studies evaluating the aptamer/HIV IN interaction highlighted the ability of the modified thymidines to establish several contacts with the target protein. All the data point to the importance of loops in the aptamer/target interaction and suggest that the site-specific replacement of loop residues with commercially available analogues can be considered a straightforward strategy to improve the biological activities of several G-quadruplex aptamers.
Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex
Authors: Bradshaw Karen P. , Figueroa Velez Dario X. , Habeeb Mariyam , Gandhi Sunil P. 
Published: May 2018

AbstractThe end of the critical period for primary visual cortex (V1) coincides with the deposition of perineuronal nets (PNN) onto Parvalbumin (PV) inhibitory neurons. Recently, we found that transplantation of embryonic inhibitory neurons into adult V1 reinstates a new critical period. Here we used Wisteria Floribunda Agglutinin (WFA) staining to compare the deposition of PNNs onto neurons during normal development and following transplantation at equivalent cell ages. In accord with previous findings, PV and PNN expression increases from negligible levels at postnatal day 14 (P14) to mature levels by P70. In contrast to P14, PNNs are found on transplanted PV neurons by 21 days after transplantation and persist to 105 days after transplantation. This precocious deposition was specific to PV neurons and excluded transplanted neurons expressing Somatostatin. Notably, the onset of PV expression in transplanted inhibitory neurons follows the timing of PV expression in juvenile V1. Moreover, transplantation has no discernible effect on host PNNs. The precocious deposition of PNNs onto transplanted PV neurons suggests that PNN expression identified by WFA does not reflect neuronal maturity and may be an inaccurate marker for transplant-induced plasticity of cortical circuits.
Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies
Authors: Golovynska Iuliia , Beregova Tatiana V. , Falalyeyeva Tatiana M. , Stepanova Ludmila I. , Golovynskyi Sergii , Qu Junle , Ohulchanskyy Tymish Y. 
Published: May 2018

AbstractThe enteric nervous system (ENS) and a glutamate receptor (GluR), N-methyl-D-aspartate receptor (NMDAR), participate in gastric acid secretion (GAS) regulation. NMDARs are localized in different stomach cells; however, knowledge of NMDAR expression and function in the ENS is limited. In the present study, we clarified the types of stomach cells that express the NMDARs that are involved in GAS regulation. The pharmacological method of isolated stomach perfusion by Ghosh and Shild combined with direct mapping of NMDARs by fluorescence microscopy in the rat stomach was employed. By immunofluorescence labeling with an anti-NMDA-NR1 antibody, NMDARs were found to be highly expressed in nerve cells of the submucosal and myenteric plexuses in the stomach. The exact localization of the NMDARs relevant to GAS and its mechanism of action were determined by stimulating different receptors of neuronal and stomach cells using specific secretagogues for NMDA and by selectively blocking those receptors. NMDARs relevant to GAS stimulation are mainly localized in cholinergic interneurons; however, all of the nerve cells of the submucosal ganglia are involved in the stimulating process. In addition, the NMDARs in parietal cells are involved in gastric acid inhibition via influencing H2-histamine receptors.