bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pulsed Electric Field Treatment Enhances the Cytotoxicity of Plasma-Activated Liquids in a Three-Dimensional Human Colorectal Cancer Cell Model
Authors: Griseti Elena , Kolosnjaj-Tabi Jelena , Gibot Laure , Fourquaux Isabelle , Rols Marie-Pierre , Yousfi Mohammed , Merbahi Nofel , Golzio Muriel 
Published: May 2019

AbstractCold atmospheric plasma and more recently, plasma-activated liquids (culture media, water or buffered solutions previously exposed to plasma), are gathering momentum in cancer cells treatment. Nevertheless, in vitro tests show that this novel approach is sometimes less efficient than expected. We here evaluate the mechanisms of action of the plasma-activated PBS and suggest to use electropermeabilization (EP) in combination with the plasma-activated phosphate-buffered saline (PBS), in order to potentiate the cytotoxic effect of the plasma activated liquid. Human multicellular tumor spheroids (MCTS), a three-dimensional cell model, which resembles small avascular tumors, was used to define the optimal treatment conditions for single and dual-mode treatments. MCTS growth, viability, and global morphological changes were assessed by live cell video-microscopy. In addition, the induction of caspases activation, the appearance of DNA damages, and cell membrane permeabilization, as well as the early modifications in the cellular ultrastructure, were examined by immunofluorescence, propidium iodide staining, confocal fluorescence microscopy and transmission electron microscopy, respectively. Altogether, our results show that a combined treatment resulted in an earlier onset of DNA damage and caspases activation, which completely abolished MCTS growth. This report is a proof of concept study evidencing that electropermeabilization greatly potentiates the cytotoxic effect of plasma-activated PBS in vitro in a three-dimensional cancer cell model.
Outcome of Septoplasty with Inferior Turbinectomy as an In-patient or Out-patient Procedure
Authors: Chen Yung-Yuan , Huang Tzu-Chin 
Published: May 2019

AbstractThe study aimed to compare the surgical outcomes of septoplasty with inferior turbinectomy between in-patient and out-patient groups. A total of 152 patients who underwent septoplasty with inferior turbinectomy between May 2012 and February 2013 were retrospectively reviewed and divided into in-patient group and out-patient group. The two groups were compared in three aspects: (i) consumption of medical resources, including National Health Insurance payments, patient surcharges, and total surgical expenses; (ii) prognostic indicators; and (iii) post-operative complications. There were no statistically significant differences between the prognostic indicators or post-operative complications in the 2 study groups except for “duration of nasal decongestant use”. The in-patient group had higher medical resource consumption in all categories. In conclusion, septoplasty with inferior turbinectomy can be performed cost-effectively as an out-patient procedure with satisfactory quality and adequate safety.
Senescence-related deterioration of intercellular junctions in the peritoneal mesothelium promotes the transmesothelial invasion of ovarian cancer cells
Authors: Pakuła Martyna , Witucka Anna , Uruski Paweł , Radziemski Artur , Moszyński Rafał , Szpurek Dariusz , Maksin Konstantin , Woźniak Aldona , Sajdak Stefan , Tykarski Andrzej , Mikuła-Pietrasik Justyna , Książek Krzysztof 
Published: May 2019

AbstractMechanisms of transmesothelial invasion of ovarian cancer are still poorly understood. Here we examined whether this phenomenon may be determined by an expression of intercellular junctions in peritoneal mesothelial cells (PMCs). Analysis of ovarian tumors showed that cancer cells are localized below an intact layer of PMCs. The PMCs located near the invaded cancer cells displayed low expression of connexin 43, E-cadherin, occludin, and desmoglein, as well as expressed SA-β-Gal, a marker of senescence. Experiments in vitro showed that senescent PMCs exhibited decreased levels of the four tested intercellular junctions, and that the invasion of ovarian cancer cells through the PMCs increased proportionally to the admixture of senescent cells. Intervention studies showed that the expression of connexin 43, E-cadherin, occludin, and desmoglein in senescent PMCs could be restored upon the blockade of p38 MAPK, NF-κB, AKT, JNK, HGF, and TGF-β1. When these molecules were neutralized, the efficiency of the transmesothelial cancer cell invasion was diminished. Collectively, our findings show that the integrity of the peritoneal mesothelium, which is determined by the expression of junctional proteins, is critical for the invasion of ovarian cancer. They also indicate a mechanism by which senescent PMCs may promote the invasive potential of cancer cells.
Minimally invasive laser treatment combined with intravitreal injection of anti-vascular endothelial growth factor for diabetic macular oedema
Authors: Inagaki Keiji , Hamada Masafumi , Ohkoshi Kishiko 
Published: May 2019

AbstractThe purpose of this study was to investigate the effect of the combination of minimally invasive laser treatment to the intravitreal injection of anti-vascular endothelial growth factor (VEGF) for diabetic macular oedema (DME). This study was retrospective longitudinal study of thirty-four eyes of 31 patients with DME. Either once or several times of intravitreal anti-VEGF injection was followed by the single minimally invasive laser within a month. The mean best corrected visual acuity (VA) and the central macular thickness (CMT) were measured before treatment, 1, 3, 6 and 12 months after the first anti-VEGF injection. The mean logMAR VA had improved from 0.52 ± 0.34 at baseline to 0.44 ± 0.32 (p = 0.003), 0.40 ± 0.34 (p = 0.006), 0.43 ± 0.33 (p = 0.063), and 0.41 ± 0.34 (p = 0.009), at 1, 3, 6, and 12 months after treatment, respectively. The mean CMT decreased significantly by 1 month and maintained over 12 months (491.1 ± 133.9 µm at baseline, 396.6 ± 116.8 µm (p = 0.001), 385.2 ± 156.2 µm (p = 0.002), 336.5 ± 86.3 µm (p = 0.000), and 354.8 ± 120.4 µm (p = 0.000) at 1, 3, 6, and 12 months, respectively). The average number of the anti-VEGF injection in 1 year was 3.6 ± 2.1 in all patients. The combined intravitreal anti-VEGF and minimally invasive laser therapy improves the VA, alleviates DME, and may decrease the required number of anti-VEGF injections.
Constitutive alterations in vesicular trafficking increase the sensitivity of cells from celiac disease patients to gliadin
Authors: Lania Giuliana , Nanayakkara Merlin , Maglio Mariantonia , Auricchio Renata , Porpora Monia , Conte Mariangela , De Matteis Maria Antonietta , Rizzo Riccardo , Luini Alberto , Discepolo Valentina , Troncone Riccardo , Auricchio Salvatore , Barone Maria Vittoria 
Published: May 2019

AbstractCeliac Disease (CD) is an autoimmune disease characterized by inflammation of the intestinal mucosa due to an immune response to wheat gliadins. Some gliadin peptides (e.g., A-gliadin P57-68) induce an adaptive Th1 pro-inflammatory response. Other gliadin peptides (e.g., A-gliadin P31-43) induce a stress/innate immune response involving interleukin 15 (IL15) and interferon α (IFN-α). In the present study, we describe a stressed/inflamed celiac cellular phenotype in enterocytes and fibroblasts probably due to an alteration in the early-recycling endosomal system. Celiac cells are more sensitive to the gliadin peptide P31-43 and IL15 than controls. This phenotype is reproduced in control cells by inducing a delay in early vesicular trafficking. This constitutive lesion might mediate the stress/innate immune response to gliadin, which can be one of the triggers of the gliadin-specific T-cell response.
Qigesan reduces the motility of esophageal cancer cells via inhibiting Gas6/Axl and NF-κB expression
Authors: Kong Lingyu , Wu Zhongbing , Zhao Yang , Lu Xin , Shi Huijuan , Liu Shugang , Li Jing 
Published: May 2019

Abstract
The present study is mainly to explore the mechanism that how Qigesan (QGS) affects the movement capacity of esophageal cancer (EC) cell. QGS incubates ECA109 and TE1 cell lines and detecting the motility of tumor cells by different experiments. Growth arrest-specific 6 (Gas6) and Anexelekto (Axl) were co-localized, and then detecting Gas6, Axl signaling pathway, and protein expression after QGS intervention. Similarly, Observing the signal localization and protein expression of P-phosphoinositide3-kinases (PI3K), P-AKT protein kinase B (AKT), P-nuclear factor-kappa B (NF-κB), matrix metalloproteinase-2 (MMP2), and matrix metalloproteinase-9 (MMP9). The results showed that the concentration of QGS was less than 200 ug/ml, and the cultured cells did not exceed 24 h, that no obvious cytotoxicity was observed. QGS significantly inhibited the mobility of ECA109 and TE1 cell lines in the concentration-dependent manner. In addition, QGS can regulate the Gas6/Axl pathway, inhibit the formation and localization of the Gas6/Axl complex, and reduce the protein activation of PI3K/AKT, NF-κB, MMP2, and MMP9. Experimental innovation shows that QGS can significantly slow down the mobility of EC cells by regulating the Gas6/Axl complex and downstream signaling pathways, and provides a theoretical basis for the pharmacological effects of QGS in the therapy of EC.
Hsa_circ_0009361 acts as the sponge of miR-582 to suppress colorectal cancer progression by regulating APC2 expression
Authors: Geng Yiting , Zheng Xiao , Hu Wenwei , Wang Qi , Xu Yanjie , He Wenting , Wu Chen , Zhu Danxia , Wu Changping , Jiang Jingting 
Published: May 2019

Abstract
Circular RNA (circRNA) plays an important role in the development of human malignant tumors. Recently, an increasing number of circRNAs have been identified and investigated in various tumors. However, the expression pattern and biological function of circRNAs in colorectal cancer (CRC) still remain largely unexplored. In the present study, hsa_circ_0009361 was significantly down-regulated in CRC tissues and cells. Low expression level of hsa_circ_0009361 promoted the proliferation, epithelial–mesenchymal transition (EMT), migration, and invasion of CRC cells. Hsa_circ_0009361 was identified as the sponge of miR-582 by fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP), and luciferase reporter assays. Overexpression of hsa_circ_0009361 up-regulated the expression of adenomatous polyposis coli 2 (APC2) and inhibited the activity of the Wnt/β-catenin pathway by competitively combining with miR-582. Exogenous miR-582 and APC2 interventions could reverse the multiple biological functions mediated by hsa_circ_0009361 in CRC cells. In vivo experiments also confirmed that hsa_circ_0009361 inhibited the growth and metastasis of CRC. Hsa_circ_0009361 acted as a tumor suppressive sponge of miR-582, which could up-regulate the expression of APC2, inhibit the Wnt/β-catenin signaling, and suppress the growth and metastasis of CRC. Collectively, the hsa_circ_0009361/miR-582/APC2 network could be employed as a potential therapeutic target for CRC patients.
Incidence of Femoral Component Malrotation Using Posterior Condylar Referencing in Total Knee Arthroplasty
Authors: Lee Diana K. , Grosso Matthew J. , Trofa David P. , Sonnenfeld Julian J. , Cooper H. John , Shah Roshan P. , Geller Jeffrey A. 
Published: May 2019

AbstractProper femoral component rotation in total knee arthroplasty (TKA) is important, given the prognostic impact of a poorly positioned component. The purpose of this observational study was to determine the incidence of femoral component malrotation using posterior condylar axis (PCA) referencing. A total of 100 knees in 92 patients with varus gonarthritis of the knee undergoing primary TKA using a standard medial parapatellar approach were evaluated intraoperatively. After distal femoral resection, the standard femoral sizing guide referencing the posterior condylar axis was used to set femoral component rotation. This was then compared with both the transepicondylar (TEA) and trochlear anteroposterior axes (TRAx). Disparites were recorded and corrected in line with the epicondylar axis. Rotational adjustment for addition of further external rotation was made in 13 (13.0%) cases. In seven cases, the medial pin sites were raised between 1 and 3 mm, and in six cases, the lateral pin site was lowered between 1 and 3 mm (based on risk of notching the femoral cortex). It is critical to not rely exclusively on the PCA to confirm rotational positioning of the femoral component as predicted by posterior condylar referencing guides. Intraoperative adjustment and confirmation using the TEA and TRAx occurred in 13% of primary TKA cases, which might have, otherwise, had a significant effect on the clinical outcome.
Chinese L2 users as active social agents: Sentence final particles variation and identities
Authors: Han Yiting 
Published: May 2019

Abstract
This study investigated the sociolinguistic pragmatic
performance of eight advanced Chinese learners from a variationist
sociolinguistic perspective, focusing on the variable appearance versus absence
of Mandarin sentence final particles (SFPs) in non-interrogative sentences.
These learners participated in a Chinese TV talk show and have become popular on
social media platforms. The speech data from the talk show (400 tokens) and
online data from Chinese social media platform Weibo (400 tokens) were collected
and analyzed. These Chinese L2 users productively demonstrated variation in
their use of SFPs in non-interrogative sentences, in that they added SFPs to a
sentence in around 20% of variable contexts. Rbrul (logistic regression)
analyses revealed that the variable presence of SFPs was conditioned by their
duration of time spent in China and gender-related personalities. Follow-up case
analyses explored how they made use of SFPs in different situations and how they
were received by other participants. The study found that these Chinese L2 users
became active social agents who made decisions to shift styles in different
contexts or to strengthen their identities. The results are discussed within a
broader context of identity, language socialization, and L2 social agency. This
study also attempts to offer pedagogical implications for the acquisition of
Chinese SFPs.
The rivalry between far from being + predicative item and its counterpart omitting the copula in Modern English
Authors: Vosberg Uwe , Rohdenburg Günter 
Published: May 2019

Abstract
Distinguishing between several subtypes of the frame far from + optional and recessive being + predicative phrase, this paper charts the evolution of the rivalling variants in British and American English over the last few centuries. The paper reports on two major findings. First, in line with the Complexity Principle, there is a tendency for more complex predicatives to help preserve the more explicit being variant better than simpler ones. In particular, morphologically complex adjectives and syntactically complex noun phrases in the predicative slot are shown to retain the being variant longer than less complex ones. Second, as regards the establishment of the less explicit zero variant, the relation between British and American English corresponds to the so-called lag and overtake scenario. While initially trailing behind British English, American English has - in more recent times - adopted the zero variant much faster than British English.
The relevance of specific semantic categories in investigating the neural bases of abstract and concrete semantics
Authors: Dreyer Felix R. , Pulvermüller Friedemann 
Published: May 2019

Abstract
Previous research has shown that modality-preferential sensorimotor areas are relevant for processing of words referring to concrete objects or actions. However, whether modality preferential areas also play a role for abstract words is still under debate. In this chapter we will argue that the apparent lack of empirical evidence for a grounding of abstract words stems, at least in part, from the treatment of abstract words as one monolithic semantic category, rather than taking into account specific abstract word types and sub-categories. We will review classical and recent empirical evidence from neuropsychological, neuroimaging and behavioral approaches and demonstrate the necessity of considering specific semantic meaning types when investigating a possible grounding of concrete and abstract concepts, for both theoretical and methodological reasons.
Acceptability properties of abstract senses in copredication
Authors: Murphy Elliot 
Published: May 2019

Abstract
This chapter explores the acceptability properties of copredication and how they can inform debates about the representation of abstract concepts. Across a series of acceptability judgment experiments, it was tested whether copredication in book-, lunch- and city-type nominals is difficult across-the-board or depends on adjective ordering in sentences like “John said that the folded and educational newspaper was on the shelf”. The results revealed no acceptability difference between copredication and non-copredication, however there was a strong preference for concrete adjectives to be placed before abstract ones. It is suggested for the first time that the parser is sensitive to semantic complexity, and that it is more optimal to access abstract concepts after associated concrete concepts than the reverse.
Different degrees of abstraction from visual cues in processing concrete nouns
Authors: Franzon Francesca , Zanini Chiara 
Published: May 2019

Abstract
Concreteness has been defined as a semantic property related to physical perception. In this paper we tackle the concreteness issue from the viewpoint of countability by arguing that uncountable expressions (e.g., some cake), although concrete, are more abstract than countable ones (e.g., one cake) since the former entail the suppression of the reference to shape, which is a salient property in the representation of entities.
We report empirical data collected with preschool children in which we show that the uncountable reference is dispreferred. We discuss possible reasons for this phenomenon, which involve the roles played by shape and by language (and in particular grammar) in early perceptual processing, and we suggest how these factors may relate to our ability to abstract from perceptual experience.
The development of the abstract scientific concept of heat energy in a naturalistic classroom setting
Authors: Zacharias Sally 
Published: May 2019

Abstract
Science aims to understand the physical and living world around us. To do this, it requires us to develop abstract ways of thinking. The aim of this chapter is to describe and explain how the abstract concepts of heat energy and heat transfer emerge and evolve during two secondary (high) school science lessons, by providing an account of how they are linguistically represented in discourse using a cognitive discursive framework. The analysis of a teacher-led demonstration, a group writing task, and an interview, during which pupils externalize their mental images in speech, writing, and in visual representations, shows how the discourse as well as the social and concrete here-and-now physical contexts, affect the development of these abstract concepts.
An outline of some future research issues for internet pragmatics
Authors: Yus Francisco 
Published: May 2019

AbstractInternet communication has evolved a lot since it first became popular in the early nineties of the last century. Pragmatics has also evolved and has tried to come to terms with the non-stop changes that internet is constantly producing in our lives and especially in how we communicate and interact. We are probably now at a stage of internet development in which we can make some sound predictions regarding certain challenges that a pragmatics of internet communication will have to face in the next few years to deal with the radical changes that are taking place in today’s internet use. This article will be devoted to listing some of these research issues and to discussing what pragmatics can do to address them accurately, ranging from those issues centred upon the interpretation of online discourses to those involving interfaces and their options for contextualisation.
The pragmatic use of vocatives in private one-to-one digital communication
Authors: Asprey Esther , Tagg Caroline 
Published: May 2019

AbstractThis article examines a corpus of private text messages collected in Birmingham and surrounding towns in 2015. We look specifically at pragmatic roles played by the vocatives we find in the corpus. Since text messages are sent to targeted recipients, vocatives are structurally redundant, and we review literature concerning vocatives in spoken and written data to first see what categories others have proposed for these functions. We then challenge some of these categorisations, identifying a new category of ‘focuser’ which we argue is akin to a summons in spoken language. We also examine the pragmatic value of vocatives in performing identity work for both sender and receiver. We finish by looking at gendered performance of identity as a case study in our corpus of how text producers construct themselves and others.
Discursive metaphorical frames in newspaper texts on language change
Authors: Bogetić Ksenija 
Published: May 2019

AbstractThe concept of ‘metaphorical framing’ is currently witnessing renewed interest in metaphor research, but for discourse-oriented work it remains a problematic analytical tool given the variety of senses it has been employed with. The present paper considers an approach to metaphorical frames in discourse, by proposing the notion ofdiscursive metaphorical framesto capture the complex, systematic metaphorical representations prominent across discourse. The perspective follows the direction of recent integrated approaches to metaphor, frames and discourse (e.g.,Burgers et al., 2016;Cameron et al., 2009;Semino et al., 2016) and is proposed as particularly suited to studying public discourses, as ideologically laden, multi-textual and multi-voiced. The approach is illustrated through an analysis of metaphorical representations of language in Serbian and British newspapers. The analysis reveals the deeper social ideologies underlying the newspaper discussions on language in Serbia and Great Britain, including similarities as well as notable differences, pointing to the diverse ideological processes shaping contemporary media metadiscourses. The results are also discussed in relation to the adopted approach, to frames of presentation, (sub)domain representations and the dynamics of metaphor use in public discourse.
The effectiveness of the Conceptual Metaphor Approach to English idiom acquisition by young Chinese learners
Authors: Pan Molly Xie 
Published: May 2019

AbstractAlthough the Conceptual Metaphor Approach (CMA) has been claimed to be effective with respect to English idiom acquisition for EFL/ESL learners at elementary level, the claim awaits further investigation with empirical evidence (Beréndi, Csábi, & Kövecses, 2008;Boers, 2004). How CMA benefits these learners’ idiom acquisition and in what way it can best be implemented in their classrooms remain unknown. This study addresses these issues and sheds light on the effectiveness of CMA through a quasi-experiment, in which metaphor is exploited as an intervention that explicitly introduces the connection between idioms and the underlying Conceptual Metaphor theme. Quantitative and qualitative data were collected from 43 participants through questionnaires, tests and a semi-structured interview with the instructor. Results from an immediate post-test showed that participants under CMA scored higher than their counterparts who were undergoing a Memorizing Translation Approach, though the difference was statistically insignificant (p = 0.08). A one-week delayed post-test found that CMA facilitated idiom acquisition at a statistically significant level (p < .05). Findings from participants’ drawings and the semi-structured interview showed that CMA had contributed to an engaging and active learning experience. Pedagogical implications of this study are discussed further.