bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome Dynamics Reveals Progressive Transition from Effector to Memory in CD4+T cells
Authors: Soon Megan S. F. , Lee Hyun Jae , Engel Jessica A. , Straube Jasmin , Thomas Bryce S. , Clarke Lachlan S. , Laohamonthonkul Pawat , Pernold Clara P. S. , Haldar Rohit N. , Williams Cameron G. , Lansink Lianne I. M. , Koufariotis Ross , Lakis Vanessa , Wood Scott , Chen Xi , James Kylie R. , Lönnberg Tapio , Lane Steven W. , Davenport Miles P. , Khoury David S. , Svensson Valentine , Teichmann Sarah A. , Haque Ashraful 
Published: June 2019

AbstractCD4+T cells are repositories of immune memory, conferring enhanced immunity to many infectious agents. Studies of acute viral and bacterial infection suggest that memory CD4+T cells develop directly from effectors. However, delineating these dynamic developmental pathways has been challenging. Here, we used high-resolution single-cell RNA-seq and temporal mixture modelling to examine the fate of Th1 and Tfh effector cells during non-lethalPlasmodiuminfection in mice. We observed linear Th1 and Tfh pathways towards memory, characterized by progressive halving in the numbers of genes expressed, and partial transcriptomic coalescence. Low-level persisting infection diverted but did not block these pathways. We observed in the Th1-pathway a linear transition from Th1 through a Tr1 state to TEMcells, which were then poised for Th1 re-call. The Tfh-pathway exhibited a modest Th1-signature throughout, with little evidence of Tr1 development, and co-expression of TCMand memory Tfh markers. Thus, we present a high-resolution atlas of transcriptome dynamics for naïve to memory transitions in CD4+T cells. We also defined a subset of memory-associated genes, including transcription factorsId2andMaf, whose expression increased progressively against the background of transcriptomic quiescence. Single-cell ATAC-seq revealed substantial heterogeneity in chromatin accessibility in single effectors, which was extensively, though incompletely reset and homogenized in memory. Our data reveal that linear transitions from effector to memory occur in a progressive manner over several weeks, suggesting opportunities for manipulating CD4+T cell memory after primary infection.HighlightsscRNA-seq reveals progressive transition from effector to memory in CD4+T cells.Transcriptome dynamics suggest linear not branching models for memory development.A subset of genes associates with gradual onset of CD4+T cell memory.Th1/Tfh predisposition varies among clonotypes with identical antigen-specificity.scATAC-seq uncovers non-coding “memory” elements in the genome.
An epitranscriptomic switch at the 5′-UTR controls genome selection during HIV-1 genomic RNA packaging
Authors: Pereira-Montecinos Camila , Toro-Ascuy Daniela , Rojas-Fuentes Cecilia , Riquelme-Barrios Sebastián , Rojas-Araya Bárbara , García-de-Gracia Francisco , Aguilera-Cortés Paulina , Ananías-Sáez Catarina , de Bisschop Grégoire , Chaniderman Jonás , Acevedo Mónica L. , Sargueil Bruno , Valiente-Echeverría Fernando , Soto-Rifo Ricardo 
Published: June 2019

ABSTRACTDuring retroviral replication, the full-length RNA serves both as mRNA and genomic RNA (gRNA). While the simple retrovirus MLV segregates its full-length RNA into two functional populations, the HIV-1 full-length RNA was proposed to exist as a single population used indistinctly for protein synthesis or packaging. However, the mechanisms by which the HIV-1 Gag protein selects the two RNA molecules that will be packaged into nascent virions remain poorly understood. Here, we demonstrate that HIV-1 full-length RNA packaging is regulated through an epitranscriptomic switch requiring demethylation of two conserved adenosine residues present within the 5′-UTR. As such, while m6A deposition by METTL3/METTL14 onto the full-length RNA was associated with increased Gag synthesis and reduced packaging, FTO-mediated demethylation was required for the incorporation of the full-length RNA into viral particles. Interestingly, HIV-1 Gag associates with the RNA demethylase FTO in the nucleus and drives full-length RNA demethylation. Finally, the specific inhibition of the FTO RNA demethylase activity suppressed HIV-1 full-length RNA packaging. Together, our data propose a novel epitranscriptomic mechanism allowing the selection of the full-length RNA molecules that will be used as viral genomes.
Consumption in fast-food restaurants in Croatia and Serbia
Authors: Sapic Srdjan , Filipovic Jovana , Dlacic Jasmina 
Published: June 2019

Purpose
The purpose of this paper is to explore the antecedents of product evaluations and behavioural intentions regarding fast-food restaurants. Research tests the moderating effects of tradition on selected relationships in Croatia and Serbia. Further, it tests consumer willingness to use services in domestic and foreign fast-food restaurants.

Design/methodology/approach
Samples were collected from 332 and 362 respondents from Croatia and Serbia, respectively. The validity of the model was tested with confirmatory factor analysis, and structural equation modelling was used to determine main and interaction effects.

Findings
For both foreign and domestic fast-food restaurants, variety seeking and cosmopolitanism had a positive impact on consumer evaluations and behavioural intentions, while the desire for unique products had a negative impact. Personal innovativeness had a positive impact on evaluations and intentions regarding foreign restaurants but a negative impact for domestic restaurants. A moderating effect of tradition was more present in the Serbian sample.

Originality/value
This study is the first to use the theory of planned behaviour and theory of social identity to investigate the impact of variety seeking, desire for unique products, cosmopolitanism and personal innovativeness on consumption in fast-food restaurants in two emerging countries in the country of origin context. Furthermore, it also provides information for researchers and marketers on fast-food consumption antecedents in different countries. New insights are provided for the moderating role of tradition.
Agility assessment in manufacturing companies
Authors: Rahiminezhad Galankashi Masoud , Helmi Syed Ahmad , Abdul Rahim Abd. Rahman , Rafiei Farimah Mokhatab 
Published: June 2019

Purpose
The purpose of this paper is to propose a framework to assess the agility of manufacturing companies.

Design/methodology/approach
Particularly, three supply chain logistic drivers (facility, transportation and inventory) along with three cross-functional drivers (information, sourcing and pricing) are selected as the main sets to classify all required activities of agility. In addition, supply chain contracts, as an important indicator of supply chain agility, is also considered to categorize the activities. These activities are ranked using an (AHP) and then categorized based on the major perspectives of agility. Finally, using a cycle view of supply chain, the developed activities are categorized as the major policies of supply chain’s echelons.

Findings
This study developed a framework to evaluate the agility of manufacturing companies. Operational activities of agile supply chain strategy (ASCS) in addition to supply chain contracts are determined and categorized with regard to supply chain drivers.

Originality/value
This study contributes to recognizing, ranking and classifying the operational activities of ASCS with regard to logistics and cross-functional drivers of supply chain. In addition, this study considers the supply chain contracts in conjunction with supply chain drivers. From the theoretic and methodological features, to the best of authors’ knowledge, this study contributes to offer new insights to this area as no similar research has been conducted before.
Story-making as a method for business modelling
Authors: Haggège Meyer , Vernay Anne-Lorène 
Published: June 2019

Purpose
Imagining a new business model is a creative process that requires entrepreneurs to define how a firm can create and capture value with a new activity. The literature emphasises various tools and approaches for prototyping business models. The purpose of this paper is to investigate the suitability of story-making as a means of designing new business models and to shed light on its potential for stimulating creative entrepreneurial thinking.

Design/methodology/approach
This paper tests the use of story-making for business modelling in a real-life case to show its usefulness and shed light on its potential for stimulating creative entrepreneurial thinking.

Findings
The authors argue that story-making should be recognised as an approach to business modelling that can foster creativity and empathy. Building on insights from design thinking literature, the paper shows that planning for a long exploratory phase is necessary to allow system thinking. It also shows that anchors can act as intermediary stopping rule and help manage complexity.

Originality/value
The paper introduces an original method for crafting business models during early stages of the innovation process and argues that this method could also be used to design business processes, especially when they are not already formalised.
Beyond national culture difference
Authors: Cui Zhipeng , Liu Junying , Xia Bo , Cheng Yaxiao 
Published: June 2019

PurposeInternational construction joint ventures (ICJVs) have been widely used as a temporary arrangement in many projects all over the world, especially in megaprojects. Within ICJVs, the national culture difference between partners affects their cooperation significantly. However, prior research has provided contradictory empirical evidence regarding these impacts. To address this problem, the purpose of this paper is to introduce cultural intelligence that judges an individual’s capability to function and manage effectively in culturally diverse settings as a moderating variable.Design/methodology/approachMultiple regression analysis and moderated multiple regression were undertaken to test proposed hypotheses. A questionnaire survey was conducted with international construction practitioners who had experiences of managing or participating in ICJVs.FindingsThe result of multiple regression analysis revealed that difference in national culture has significant negative effects on information exchange, shared problem solving and flexibility when asking for changes, thus effecting cooperation within ICJVs. Meanwhile, cultural intelligence of members can weaken these negative influences.Practical implicationsFirst, given that national culture difference affects negatively on the cooperation within ICJVs, it needs to be regarded as one of vital resources of risk which should be prevented and managed when attending ICJVs; Second, managers should build a series of mutually agreeable regulations and rules to lessen the negative effect of national culture difference; Third, it is recommended that ICJV management teams contain as many work-experienced members as possible and members within ICJVs, especially new staff, receive cross-cultural training termly to facilitate the cooperation between partners.Originality/valueThis research reveals the moderating effects of cultural intelligence on the relationship between national culture difference and cooperation in ICJVs as well as provides practical implications for ICJV managers to deal with national culture difference and reduce its negative impact on cooperation within ICJVs.
How financial systems and firm strategy impact the choice of innovation funding
Authors: Sierra Jaime 
Published: June 2019

PurposeThe funding of innovation is explained by typical cost-based financial approaches. This paper breaks away from such tradition, and the purpose of this paper is to propose an alternative view where innovation funding decisions are strategic and concern interactions between actors – each with their own characteristics and strategic intentions – project features, and traits of the setting in which interactions take place.Design/methodology/approachThis paper builds up an alternative framework to understand how innovation is financed by considering the interplay of innovation characteristics, the strategic reasons of project owners and funders, and the role of the matching environment and conditions. This proposal includes explanatory elements overlooked by extant theories. An illustrative case is presented to support the need for this proposal.FindingsThe framework proposed proves useful to better understand innovation funding cases where the traditional financial theory does not suffice.Practical implicationsInnovative companies may improve decision making about resource allocation to innovation; innovation funders may refine their decision-making criteria and implementation; and policy makers and practitioners need to devise better supporting strategies for innovative companies.Originality/valueThis proposal considers a continuum of funding options where supply/demand will match on the grounds of strategic decisions made during the interaction itself, under certain contextual conditions. Hence, it enriches the understanding of strategic decisions regarding firm capital structure and investment theory when it comes to funding innovation.
Consumer engagement in online brand communities: the moderating role of personal values
Authors: Marbach Julia , Lages Cristiana , Nunan Daniel , Ekinci Yuksel 
Published: June 2019

Purpose
Despite growing recognition of the importance of consumer engagement with new technologies, a gap remains in terms of understanding the antecedents, consequences and moderators of online consumer engagement (OCE). This paper aims to address this gap by exploring the relationship between personality traits, OCE, perceived value and the moderating role of personal values.

Design/methodology/approach
A theoretical framework anchored in the extant OCE literature is tested through a study of 559 users of two distinct firm-hosted online brand communities (FHOBCs).

Findings
Findings suggest that three personality traits – extraversion, openness to experiences and altruism – are positively correlated with OCE. OCE is related to two types of perceived value, namely, social value and aesthetic value. The personal values of conservation and self-enhancement moderate the relationships between the three identified personality traits and OCE.

Research limitations/implications
Future research into OCE should consider the application of this study’s conceptual framework across different cultures to account for the fast-changing nature of online communities.

Practical implications
Understanding how personality traits drive OCE and what value consumers receive from engagement in online communities can help managers to better segment and evaluate consumers. Engagement and levels of activity within these online communities can be improved accordingly.

Originality/value
This study’s contribution to the OCE literature is threefold. First, the study provides new insights regarding personality traits as antecedents of consumer engagement with FHOBCs. Second, the study reveals the first insights into the role of personal values in the relationship between personality traits and OCE. Specifically, conservation and self-enhancement emerged as moderators of the relationship between three personality traits (extraversion, openness to experiences, altruism) and OCE. Third, the study yields support for perceived value types (social value and aesthetic value) that emerge as consequences of consumer engagement in FHOBCs.
Barriers to “green operation” of commercial office buildings
Authors: Rock Stephanie , Hosseini M. Reza , Nikmehr Bahareh , Martek Igor , Abrishami Sepehr , Durdyev Serdar 
Published: June 2019

Purpose
The built environment is a major source of carbon emissions. However, 80 per cent of the damage arises through the operational phase of a building’s life. Office buildings are the most significant building type in terms of emission-reduction potential. Yet, little research has been undertaken to examine the barriers faced by building operators in transitioning to a green operation of the office buildings in their care. This study aims to identify those barriers.

Design/methodology/approach
Building facilities managers with between 7 and 25 years’ experience in operating primarily Melbourne high-rise office buildings were interviewed. The sample was taken from LinkedIn connections, with ten agreeing to participate in semi-structured interviews – out of the 17 invitations sent out. Interview comments were recorded, coded and categorised to identify the barriers sought by this study.

Findings
Seven categories of barriers to effecting green operation of office buildings were extracted. These were financial, owner-related, tenant-related, technological, regulatory, architectural and stakeholder interest conflicts. Difficulties identifying green operation strategies that improved cost performance or return on investment of buildings was the major barrier.

Practical implications
Government, policymakers and facilities managers themselves have been struggling with how to catalyse a green transition in the operation of office buildings. By identifying the barriers standing in the way, this study provides a concrete point of departure from which remedial strategies and policies may be formulated and put into effect.

Originality/value
The uptake of green operation of office buildings has been extremely slow. Though barriers have been hypothesised in earlier works, this is the first study, to the best of the authors’ knowledge, that categorically identifies and tabulates the barriers that stand in the way of improving the green operational performance of office buildings, drawing on the direct knowledge of facilities experts.
Simulation of water/FMWCNT nanofluid forced convection in a microchannel filled with porous material under slip velocity and temperature jump boundary conditions
Authors: Gholamalizadeh Ehsan , Pahlevanzadeh Farzad , Ghani Kamal , Karimipour Arash , Nguyen Truong Khang , Safaei Mohammad Reza 
Published: June 2019

Purpose
This study aims to numerically study the forced convection effects on a two-dimensional microchannel filled with a porous material containing the water/FMWCNT nanofluid. The upper and lower microchannel walls were fully insulated thermally along 15 per cent of their lengths at each end of the microchannel, with the in-between length being exposed to a constant temperature. The slip velocity boundary condition was applied along the microchannel walls.

Design/methodology/approach
The Navier–Stokes equations were discretized before being solved numerically via a FORTRAN computer code. The following ranges were considered for the studied parameters: slip factor (B) equal to 0.001, 0.01 and 0.1; Reynolds number (Re) between 10 and 100; solid nanoparticle mass fraction (ϕ) between 0.0012 and 0.0025; Darcy number (Da) between 0.001 and 0.1; and porosity factor (ε) between 0.4 and 0.9.

Findings
Increasing the Da caused a greater increase in the velocity profile than increasing Re, whereas increasing porosity did not affect the velocity profile growth at all.

Originality/value
This paper is the continuation of the authors’ previous studies. Using the water/FMWCNT nanofluid as the working fluid in microchannels is among the achievements of this study.
Liquid drop breakup in homogeneous isotropic turbulence
Authors: Zhong Cheng , Komrakova Alexandra 
Published: June 2019

Purpose
This paper aims to demonstrate the capabilities of a diffuse interface free energy lattice Boltzmann method to perform direct numerical simulations of liquid–liquid dispersions in a well-controlled turbulent environment. The goal of this research study is to develop numerical techniques that can visualize and quantify drop interaction with the turbulent vortices. The obtained information will be used for the development of sub-models of drop breakup for multi-scale simulations.

Design/methodology/approach
A pure binary liquid system is considered that is subject to fully developed statistically stationary turbulent flow field in a cubic fully periodic box with the edge size of 300 lattice units. Three turbulent flow fields with varying energy input are examined and their coherent structures are visualized using a normalized Q-criterion. The evolution of the liquid–liquid interface is tracked as a function of time. The detailed explanation of the numerical method is provided with a highlight on a choice of the numerical parameters.

Findings
Drop breakup mechanisms differ depending on energy input. Drops break due to interaction with the vortices. Quantification of turbulent structures shows that the size of vortices increases with the decrease of energy input. Drop interacts simultaneously with multiple vortices of the size comparable to or smaller than the drop size. Vortices of the size smaller than the drop size disturb drop interface and pinch off the satellites. Vortices of the size comparable to the drop size tend to elongate the drop and tear it apart producing daughter drops and satellites. Addition of the second phase enhances turbulent dissipation at the high wavenumbers. To obtain physically realistic two-phase energy spectra, the multiple-relaxation-time collision operator should be used.

Originality/value
Detailed information of drop breakup in the turbulent flow field is crucial for the development of drop breakup sub-models that are necessary for multi-scale numerical simulations. The improvement of numerical methods that can provide these data and produce reliable results is important. This work made one step towards a better understanding of how drops interact with the turbulent vortices.
Numerical modeling of aeroacoustic characteristics of different savonius blade profiles
Authors: Delbari Seyed Hamid , Nejat Amir , Ahmadi Mohammad H. , Khaleghi Ali , Goodarzi Marjan 
Published: June 2019

Purpose
This study aims to carry out numerical modeling to predict aerodynamic noise radiation from four different Savonius rotor blade profile.

Design/methodology/approach
Incompressible unsteady reynolds-averaged navier-stokes (URANS) approach using gamma–theta turbulence model is conducted to obtain the time accurate turbulent flow field. The Ffowcs Williams and Hawkings (FW-H) acoustic analogy formulation is used for noise predictions at optimal tip speed ratio (TSR).

Findings
The mean torque and power coefficients are compared with the experimental data and acceptable agreement is observed. The total and Mono+Dipole noise graphs are presented. A discrete tonal component at low frequencies in all graphs is attributed to the blade passing frequency at the given TSR. According to the noise prediction results, Bach type rotor has the lowest level of noise emission. The effect of TSR on the noise level from the Bach rotor is investigated. A direct relation between angular velocity and the noise emission is found.

Practical implications
The savonius rotor is a type of vertical axis wind turbines suited for mounting in the vicinity of residential areas. Also, wind turbines wherein operation are efficient sources of tonal and broadband noises and affect the inhabitable environment adversely. Therefore, the acoustic pollution assessment is essential for the installation of wind turbines in residential areas.

Originality/value
This study aims to investigate the radiated noise level of four common Savonius rotor blade profiles, namely, Bach type, Benesh type, semi-elliptic and conventional. As stated above, numbers of studies exploit the URANS method coupled with the FW-H analogy to predict the aeroacoustics behavior of wind turbines. Therefore, this approach is chosen in this research to deal with the aeroacoustics and aerodynamic calculation of the flow field around the aforementioned Savonius blade profiles. The effect of optimal TSR on the emitted noise and the contribution of thickness, loading and quadrupole sources are of interest in this study.