bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal undernutrition in the first eighty days of gestation negatively programs ovarian development in dairy calves
Authors: Frau Adele , Edache David , Sale Sebastiano , Gallo Antonio , Miragliotta Vincenzo , Lazzarini Giulia , Corda Andrea , Corda Francesca , Barbato Olimpia , Succu Sara , Bebbere Daniela , Franciosi Federica , Atzori Alberto S , Mossa Francesca 
Published: November 2024

Abstract
We hypothesized that in dairy cattle maternal energy restriction applied during two gestational windows (up to day 80 or 120 of gestation) impairs ovarian and cardiovascular development in juvenile female offspring. We also investigated the role of maternal leptin and testosterone in developmental programming in calves. Holstein-Friesian heifers were randomly assigned to one of three experimental groups; starting 10 days before artificial insemination, they were individually fed at (i) 0.6 of their maintenance energy requirements (M) up to day 80 (Nutrient Restricted, NR80) or (ii) day 120 of gestation (NR120); (iii) 1.8 M until day 120 of pregnancy (Control). Plasma leptin concentrations increased transiently in nutritionally restricted heifers pregnant with a single female calf, but maternal testosterone concentrations were not influenced by diet. Calves had similar body growth, but daughters of NR80 and NR120 had impaired ovarian development, as assessed by reduced gonadal weight, fewer surface antral and primary follicles, and recovered cumulus-oocyte complexes, as well as lower circulating anti-Müllerian hormone concentrations. Cardiovascular morphology and function in the offspring were not influenced by maternal diet, as determined by peripheral arterial blood pressure, echocardiography, post-mortem heart weight, and aortic circumference. Regardless of its duration (until day 80 or 120 of gestation), nutritional restriction resulted in a similar alteration of ovarian development in juvenile progeny, but cardiovascular development was unaltered. Evidence suggests that the window of development that encompasses the peri-ovulatory period to the first 2.6 months of gestation is critical in ovarian programming and that maternal leptin may be involved.
Management of knee osteoarthritis using bone marrow aspirate concentrate: a systematic review
Authors: Migliorini Filippo , Pilone Marco , Ascani Jacopo , Schäfer Luise , Jeyaraman Madhan , Maffulli Nicola 
Published: November 2024

Abstract

Introduction
Knee osteoarthritis (OA) is a common degenerative joint condition and a major cause of disability in the general population.

Source of data
Recent published literature identified from PubMed, EMBASE, Google Scholar, and Scopus.

Areas of agreement
Orthobiological therapies try to regenerate articular cartilage and stop the progression of the degenerative lesion. Intra-articular injections of biological derivates have been increasingly used in the last decade.

Areas of controversy
The indications for the use of bone marrow aspirate concentrate (BMAC) are still unclear.

Growing points
We systematically reviewed the current literature on BMAC in the management of knee OA, giving an update on the current indications for the selection of the ideal patient and the preparations and efficacy of BMAC compared to other biological alternatives.

Areas timely for developing research
BMAC is a valuable source of mesenchymal stem cells, offering potential benefits in attenuating the inflammatory pathway associated with knee OA. Intra-articular injection of BMAC has shown effectiveness in clinical trials improving functional outcomes of knee OA patients. The superiority of BMAC over other orthobiological treatments cannot be assessed because of conflicting results.
Online transcranial magnetic stimulation reveals dynamic interactions between language control and processing in bilingual language production
Authors: Wu Junjie , Ji Yannan , Cai Chuyao , Pu Xinping , Wang Qiping , Yan Guoli , Wang Qiang , Wang Xin 
Published: November 2024

Abstract
Language production in bilinguals relies on the collaborative interaction between two neural systems: the language control system (e.g. the right inferior frontal gyrus) and the language processing system (e.g. the left superior temporal gyrus). However, the causal mapping between these brain systems and their cognitive functions, as well as the temporal dynamics between these two systems during bilingual language production, remain unclear. To investigate this, our study applied online transcranial magnetic stimulation to the right inferior frontal gyrus and left superior temporal gyrus in Chinese–English bilinguals performing a language-switching task. Transcranial magnetic stimulation was administered within seven 100 ms time windows (TW1 to TW7) following the picture onset. We observed time window–specific enhancements of language control, as shown by a significant reduction in switching costs upon the stimulations on both sites relative to the sham stimulation: specifically, the right inferior frontal gyrus at TW1, TW2, TW4, and TW5 and the left superior temporal gyrus at TW2 and TW5. These findings indicate a double-strike top–down control mechanism underpinned by the pathway from the right inferior frontal gyrus to the right inferior frontal gyrus, potentially during both stages during production: the language task schema and the lemma selection. Our study provides the first evidence of the dynamic interactions between language control and processing systems causally underpinning bilingual language production.
Survival after extracorporeal cardiopulmonary resuscitation for adolescent arrhythmogenic arrest: ECPella (extracorporeal membrane oxygenation with Impella®)—a case report
Authors: Lerner Anouska , Desai Ajay , Trimlett Richard , Till Janice , Chan-Dominy Amy 
Published: November 2024

Abstract

Background
The combined therapy with venoarterial extracorporeal membrane oxygenation and Impella (ECPella) has been shown to improve survival in acute cardiogenic shock (CS) in adult patient. Only three paediatric cases have been reported in a multicentre study.

Case summary
We present our case, the first described to our knowledge in the UK, of a 15-year-old adolescent of Afro-Caribbean descent, weight 75 kg, who received extracorporeal cardiopulmonary resuscitation (E-CPR) and ECPella implantation. The patient suffered a sudden cardiac arrest at home; his mother called for an ambulance that arrived within 10 min and commenced life support with a LUCAS device. He received three doses of adrenaline and three desynchronized shocks for an underlying rhythm of ventricular fibrillation (VF) after which return of spontaneous circulation was achieved. He was then transferred to his local hospital where he had another VF arrest with successful cardioversion and was then transferred to our institution where on arrival he had another VF arrest and received E-CPR and ECPella implantation under the institutional adult shock programme within 3 h of in-hospital cardiac arrest. Following weaning from ECPella, the patient underwent cardiac and brain magnetic resonance imaging and serial echocardiograms with complete recovery of ventricular function. After implantation of cardiac defibrillator, he was discharged home without neurological sequelae. He remains asymptomatic from a cardiac perspective, with a normal cardiac examination and with no neurological sequelae at 2-year follow-up.

Discussion
This is the first case description of ECPella use in a child in the UK and highlighted the importance of timely institution of E-CPR on survival benefit in fatal CS. The outcome success of post-resuscitation ECPella strategy in this adolescent was through collaborative interprofessional engagement of multiple supra-specialists within acute cardiology and critical care across paediatric and adult services and alignment with the institutional adult shock programme.
Tracking the uptake of labelled host-derived extracellular vesicles by the human fungal pathogen Aspergillus fumigatus
Authors: Visser Corissa , Rivieccio Flora , Krüger Thomas , Schmidt Franziska , Cseresnyés Zoltán , Rohde Manfred , Figge Marc Thilo , Kniemeyer Olaf , Blango Matthew G , Brakhage Axel A 
Published: November 2024

Abstract
Extracellular vesicles (EVs) have gained attention as facilitators of intercellular as well as interkingdom communication during host–microbe interactions. Recently we showed that upon infection, host polymorphonuclear leukocytes produce antifungal EVs targeting the clinically important fungal pathogen Aspergillus fumigatus; however, the small size of EVs (&lt;1 µm) complicates their functional analysis. Here, we employed a more tractable, reporter-based system to label host alveolar epithelial cell-derived EVs and enable their visualization during in vitro A. fumigatus interaction. Fusion of EV marker proteins (CD63, CD9, and CD81) with a Nanoluciferase (NLuc) and a green fluorescent protein (GFP) facilitated their relative quantification by luminescence and visualization by a fluorescence signal. The use of an NLuc fused with a GFP is advantageous as it allows for quantification and visualization of EVs simultaneously without additional external manipulation and to distinguish subpopulations of EVs. Using this system, visualization and tracking of EVs was possible using confocal laser scanning microscopy and advanced imaging analysis. These experiments revealed the propensity of host cell-derived EVs to associate with the fungal cell wall and ultimately colocalize with the cell membrane of A. fumigatus hyphae in large numbers. In conclusion, we have created a series of tools to better define the complex interplay of host-derived EVs with microbial pathogens.
Hyperactivation of the PI3K pathway in inborn errors of immunity: current understanding and therapeutic perspectives
Authors: IJspeert Hanna , Dalm Virgil A S H , van Zelm Menno C , Edwards Emily S J 
Published: November 2024

Abstract
The phosphoinositide-3-kinase (PI3K) pathway function is crucial to the normal development, differentiation, and function of immune cells including B, T, and NK cells. Following the description of two cohorts of patients with an inboirn error of immunity (also known as primary immunodeficiency) with gain-of-function variants in the PIK3CD gene a decade ago, the disease entity activated PI3K delta syndrome (APDS) was named. Since then, many more patients with PIK3CD variants have been described, and loss-of-function variants in PIK3R1 and PTEN have also been linked to APDS. Importantly, the availability of small molecules that inhibit the PI3K pathway has enabled targeted treatment of APDS patients. In this review, we define (i) the PI3K pathway and its role in inborn errors of immunity; (ii) the clinical and immunological presentation of APDS1 (PIK3CD GOF), APDS2 (PIK3R1 LOF), and related disorders; (iii) Diagnostic approaches to identify and functionally validate the genetic causes of disease; (iv) therapeutic interventions to target PI3K hyperactivation; and finally (v) current challenges and future perspectives that require attention for the optimal treatment of patients with APDS and APDS-L diseases.
Saccharomyces boulardii enhances anti-inflammatory effectors and AhR activation via metabolic interactions in probiotic communities
Authors: Hedin Karl Alex , Mirhakkak Mohammad H , Vaaben Troels Holger , Sands Carmen , Pedersen Mikael , Baker Adam , Vazquez-Uribe Ruben , Schäuble Sascha , Panagiotou Gianni , Wellejus Anja , Sommer Morten Otto Alexander 
Published: November 2024

Abstract
Metabolic exchanges between strains in gut microbial communities shape their composition and interactions with the host. This study investigates the metabolic synergy between potential probiotic bacteria and Saccharomyces boulardii, aiming to enhance anti-inflammatory effects within a multi-species probiotic community. By screening a collection of 85 potential probiotic bacterial strains, we identified two strains that demonstrated a synergistic relationship with S. boulardii in pairwise co-cultivation. Furthermore, we computationally predicted cooperative communities with symbiotic relationships between S. boulardii and these bacteria. Experimental validation of 28 communities highlighted the role of S. boulardii as a key player in microbial communities, significantly boosting the community’s cell number and production of anti-inflammatory effectors, thereby affirming its essential role in improving symbiotic dynamics. Based on our observation, one defined community significantly activated the aryl hydrocarbon receptor—a key regulator of immune response—280-fold more effectively than the community without S. boulardii. This study underscores the potential of microbial communities for the design of more effective probiotic formulations.
Dose-efficient phase-contrast imaging of thick weak phase objects via OBF STEM using a pixelated detector
Authors: Ooe Kousuke , Seki Takehito , Nogami Mitsuru , Ikuhara Yuichi , Shibata Naoya 
Published: November 2024

Abstract
Optimum bright-field scanning transmission electron microscopy (OBF STEM) is a recently developed low-dose imaging technique that uses a segmented or pixelated detector. While we previously reported that OBF STEM with a segmented detector has a higher efficiency than conventional STEM techniques such as annular bright field (ABF), the imaging efficiency is expected to be further improved by using a pixelated detector. In this study, we adopted a pixelated detector for the OBF technique and investigated the imaging characteristics. Because OBF imaging is based on the thick weak phase object approximation (tWPOA), a non-zero crystalline sample thickness is considered in addition to the conventional WPOA, where the pixelated OBF method can be regarded as the theoretical extension of single side band (SSB) ptychography. Thus, we compared these two techniques via signal-to-noise ratio transfer functions (SNRTFs), multi-slice image simulations, and experiments, showing how the OBF technique can improve dose efficiency from the conventional WPOA-based ptychographic imaging.
Detection of Gram-positive and Gram-negative bacteria in brain abscesses by 16S rRNA in situ hybridization
Authors: Mbongo William , Laga Alvaro C , Solomon Isaac H 
Published: November 2024

Abstract
In situ hybridization (ISH) staining of bacterial 16S ribosomal RNA (rRNA) is an alternative to standard histological stains (eg, Gram, Warthin-Starry), and may improve the diagnosis of bacterial brain abscesses. To evaluate the utility of 16S rRNA ISH, a 10-year retrospective cohort was assembled from a large academic medical center. Results of histological stains, cultures, and 16S rRNA sequencing were extracted from reports, and new Gram and 16S rRNA ISH stains were performed. Histologically identifiable bacteria were present in 40/63 (63%) cases and 38/57 (67%) were associated with positive cultures. Overall, 16S rRNA ISH was positive in 18/63 (29%) cases, including 16/37 (43%) with positive Gram stains, 12/38 (32%) positive by culture, and 4/8 (50%) positive by sequencing. 16S rRNA ISH highlighted bacteria in 14/40 (35%) cases with Gram-positive organisms and 9/17 (53%) with Gram-negative organisms (including 6 polymicrobial cases). Compared to a composite gold standard of Gram stain and culture, the sensitivity and specificity of 16S rRNA ISH were 35% and 93%, respectively. While sensitivity is relatively low, 16S rRNA ISH may be useful for distinguishing real organisms from artifacts and for identifying brain abscess cases suitable for 16S rRNA sequencing.
Case report: Migratory biliary stent resulting in sigmoid colon perforation
Authors: Beloy Jasmine B , Lund Nicholas P , Van Hell Annika M , Allamaneni Shyam 
Published: November 2024

Abstract
Biliary stent insertion during endoscopic retrograde cholangiopancreatography is a therapeutic intervention to relieve obstruction and facilitate flow through the biliary tree. In rare circumstances, these stents can migrate and result in distal gastrointestinal perforation, which may necessitate endoscopic or surgical intervention. We report a case involving a 79-year-old female who presented with peritonitis due to sigmoid colon perforation following biliary stent migration. The stent was placed to treat acute cholangitis with choledocholithiasis. Two weeks following stent placement, gastroenterology attempted scheduled stent removal, but was unable to visualize the stent on endoscopy. Eleven days later, the patient was emergently taken to the operating room for an exploratory laparotomy and a Hartmann’s procedure for stent migration and subsequent sigmoid perforation. No established protocol exists for managing migratory biliary stents to avoid perforations. We emphasize the need for follow-up imaging and individualized clinical decision-making based on patient stability.
Nash meets Łukasiewicz: computing equilibria through logic
Authors: Preto Sandro , Finger Marcelo 
Published: November 2024

Abstract
A Nash equilibrium is a strategy profile of a game in which none of the players involved has any gain by changing alone his/her own strategy. Given a two-player game, we show a codification of all of its Nash equilibria into Łukasiewicz infinitely-valued logic, that is, we derive a propositional theory in this logic whose models codify all the Nash equilibria. Based on such propositional theory, we derive a polynomial reduction from the problem of computing a Nash equilibrium to the problem of satisfiability of (sets of) formulas of Łukasiewicz infinitely-valued logic. These applications of logic to game theory lead to new methods for computing Nash equilibria.
Combat-Relevant Anesthesia Fellowships Help Sustain the Army’s Multidomain Medical Force
Authors: Custer Jonathan 
Published: November 2024

ABSTRACT
In the community of Army Nurse Anesthetists, there is an underutilized potential for Combat-Relevant Fellowship training that, if enthusiastically encouraged and taken advantage of, would proffer many benefits to the Military Health System and the beneficiaries of its care. Most importantly, increased access to and encouragement for participation in these fellowships for Nurse Anesthetists would act as a tool for skill sustainment, denying any potential for skill degradation or readiness gaps in preparation for the next-generation war. These fellowships would also augment the already robust breadth, quality, and safety of Army Nurse Anesthetists’ combat and noncombat anesthesia care, potentially increase Return-To-Duty rates, potentially expedite casualty clearance of the battlefield, increase the often-limited access to advanced pain management care for chronic pain patients in Military Treatment Facilities, increase the retention rates of Nurse Anesthetists, augment the military anesthesia community’s knowledge-base, and help to advance the art of anesthesia as a whole. To triumph against the anticipated rigors of the future war’s multidomain operational environment, we are duty bound to continually improve and strive to be the best versions of ourselves as soldiers, as professionals, and as communities. This paper will explore the untapped utility of Combat-Relevant Fellowships for Army Nurse Anesthetists.
Preparing Military Interprofessional Health Care Teams for Effective Collaboration
Authors: Barry Erin S , Varpio Lara , Teunissen Pim , Vietor Robert , Kiger Michelle 
Published: November 2024

ABSTRACT

Introduction
Military Interprofessional Health care Teams (MIHTs) are the backbone of modern military medicine. However, these teams face distinct operational challenges, including frequent personnel rotation, diverse work environments, and the constant possibility of rapid deployment. Serving in dynamic teams that deploy for both military and humanitarian missions, MIHTs face the unique challenge of constant restructuring and reorganization. Consequently, preparing MIHT members to function effectively as a team presents a significant hurdle. This difficulty highlights the limited applicability of existing literature focused on training civilian interprofessional health care teams. To address this gap, we conducted interviews with MIHT members to understand specific training elements that equip MIHT members to effectively collaborate. By gaining a deeper understanding of their needs, we can improve training programs and ultimately optimize MIHT performance, readiness, and patient care.

Methods
We conducted individual semi-structured interviews with military health care professionals. We employed purposeful sampling to ensure a diverse range of perspectives from individuals with direct experience working in or leading MIHTs. The 30 participants interviewed represented a broad spectrum of MIHT professions. The data used for this study stems from a broader research program on MIHTs conducted between 2017 and 2019. We conducted a secondary analysis focusing specifically on interview data related to education and training. Using Braun and Clarke’s 6-step approach to Thematic Analysis, we identified themes from the data to build an understanding of MIHT perspectives on training effectiveness.

Results
The participants’ insights allowed us to identify 3 critical themes related to the training elements they considered most beneficial for fostering collaboration within MIHTs: (1) MIHT members rely on their own predeployment readiness; (2) MIHT contexts require unique, adaptive communication skills; and (3) MIHT training is an ongoing endeavor.

Discussion
We need our MIHTs to be ready to deploy and effectively collaborate, which involves being clinically, emotionally, physically, and operationally ready. MIHT members describe 3 aspects of specific training elements as having significant potential to equip them to effectively collaborate: (1) MIHT members rely on their own predeployment readiness; (2) MIHT contexts require unique, adaptive communication skills; and (3) MIHT training is an ongoing endeavor at both the individual and team levels. By investing in tailored training programs that address these areas, we can empower MIHTs to continuously adapt, excel, and ultimately, positively impact patient outcomes in diverse military health care settings.
The X-ray re-brightening of GRB afterglow revisited: a possible signature from activity of the central engine
Authors: Yang Zhe , Lü Hou-Jun , Yang Xing , Shen Jun , Yi Shuang-Xi 
Published: November 2024

ABSTRACT
Long-duration gamma-ray bursts (GRBs) are thought to be from core collapse of massive stars, and a rapidly spinning magnetar or black hole may be formed as the central engine. The extended emission in the prompt emission, flares, and plateaus in X-ray afterglow, are proposed to be as the signature of central engine re-activity. However, the direct evidence from observations of identifying the central engines remains an open question. In this paper, we systemically search for long-duration GRBs that consist of bumps in X-ray afterglow detected by Swift/XRT and find that the peak time of the X-ray bumps exhibit bimodal distribution (defined as ‘early’ and ‘late’ bumps) with division line at $t=7190$ s. Although we cannot rule out that such a bimodality arises from selection effects. We proposed that the long-duration GRBs with an early (or late) bumps may be originated from the fall-back accretion onto a new-born magnetar (or black hole). By adopting Monte Carlo Markov Chain (MCMC) method to fit the early (or late) bumps of X-ray afterglow with the fall-back accretion of magnetar (or black hole), it is found that the initial surface magnetic field and period of magnetars for most early bumps are clustered around $5.88\times 10^{13}$ G and 1.04 ms, respectively. Meanwhile, the derived accretion mass of black hole for late bumps is in the range of $[4\times 10^{-4}, 1.8\times 10^{-2}]\,{\rm M}_{\odot }$, and the typical fall-back radius is distributed range of $[1.04, 4.23]\times 10^{11}$ cm, which is consistent with the typical radius of a Wolf–Rayet star. However, we also find that the fall-back accretion magnetar model is disfavoured by the late bumps, but the fall-back accretion of black hole model cannot be ruled out to interpret the early bumps of X-ray afterglow.