bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved Quality Attributes in Soy Yogurts Prepared From DAG Enriched Edible Oils and Edible Deoiled Soy Flour
Authors: Sengupta Samadrita , Bhattacharyya Dipak K. , Bhowal Jayati 
Published: June 2018

Diacylglycerols (DAGs), particularly the 1, 3‐isoform, have immense potential health benefits with regards to post prandial lipemia and obesity. DAG enriched edible oil fortified soy yogurts are developed using edible decorticated and deoiled soy flour instead of using whole soybean seed. DAG enriched oils are prepared by enzymatic glycerolysis from different edible oils namely soybean, sunflower, rice bran, mustard, and sesame oil. All DAG oil enriched soy yogurts exhibit higher oxidative stability and antioxidant activity than the control soy yogurt during storage at 4 °C. The extent of proteolysis by Lactobacillus spp. and Streptococcus spp. is enhanced by the presence of DAG‐enriched edible oils as shown by a substantial increase in o‐phthaldialdehyde values. The results exhibit that the addition of rice bran oil‐DAG improves the physicochemical properties, microbiological as well as proteolytic activity of soy yogurts and enables a novel technological approach for making fortified soy yogurts with enhanced health‐benefit ingredients.Practical application: DAG enriched edible oils may be considered as novel functional food ingredients for soy yogurt production. Thus, development of functional soy yogurt with DAG enriched edible oils is an alternative for a value‐added product with better physicochemical properties, microbiological as well as proteolytic activity compared with conventional soy yogurts. The process is commercially economical in view of the uses of seed flour as direct raw material without odor problems for making non‐dairy food products. Soy yogurt with DAG enriched edible vegetable oils can be protected from adverse external conditions with potential benefits in food and therapeutic formulations for improved efficacy and health benefits.Soy yogurts prepared from diaclyglycerol enriched edible oils can be a novel approach for soy yogurt production. The process is economical and can be shown to have improved health benefits in the final product.
Impaired Signaling of NF-κB and NRF2 in CX3CR1-Deficient Microglia: Implications in Tauopathies
Authors: Castro-Sánchez Sara , García-Yagüe Ángel J. , Kügler Sebastian , Lastres-Becker Isabel 
Published: June 2018

ABSTRACTTAU protein aggregation is the main characteristic of neurodegenerative diseases known as tauopathies. Low-grade chronic inflammation is also another hallmark that indicates crosstalk between damaged neurons and glial cells. We have demonstrated that neurons overexpressing TAUP301L release CX3CL1, which activates anti-inflammatory NRF2 signalling in microglial cells in vitro and in vivo. However, the potential role of CX3CR1 in the context of tauopathies and its implication in neuroinflammation are poorly described. In this work we show that CX3CL1 activates the pro-inflammatory pathway as an early response mediated by the transcription factor NF-κB through the activation of mitogen-and stress-activated protein kinase-1 (MSK-1). At a functional level, CX3CR1-deficient microglia show decreased expression of NRF2, impaired cell migration and deficiency of phagocytosis. The relevance of these findings is evident in a tauopathy model, where the treatment with an inducer of NRF2, sulforaphane, is able to modulate astrogliosis but not microgliosis. These findings suggest that CX3CR1/NRF2 axis is essential in microglial activation associated with tauopathies and that polymorphisms have to be taken into account to development of therapeutic strategies
Four assessment methods to measure student gains in a graduate course on mathematical modeling in cell biology
Authors: Dale Renee , Kato Naohiro , Wischusen Bill 
Published: June 2018

ABSTRACTThe push for mathematics and quantitative skill development in biology is greater than ever before. The perceptions of these techniques are a barrier to their implementation on a grand scale. Methods to assess the efficacy of approaches to teaching quantitative skills are needed to determine if they effectively overcome social stereotypes and fears of math. In our project we study the effect of a mathematical modeling course in cell biology. We developed four assessments to understand if hands-on modeling increases student understanding of the concepts while simultaneously reducing fear of math. Two concept inventories track students’ learning gains both in general and specific knowledge to the course content. Two surveys quantify students’ quantitative confidence and opinions of the usefulness of these techniques in biology. We discuss the effectiveness of our methods and the implications for assessment development in mathematical biology education.
Genomic divergence in allopatric Northern Cardinals of the North American warm deserts is associated with behavioral differentiation
Authors: Provost Kaiya L. , Mauck William M. , Tilston Smith Brian 
Published: June 2018

ABSTRACTBiogeographic barriers are thought to be important in initiating speciation through geographic isolation, but they rarely indiscriminately and completely reduce gene flow across the entire community. Understanding which species’ attributes regulate a barrier could help elucidate how speciation is initiated. Here, we investigated the association of behavioral isolation on population differentiation in Northern Cardinals (Cardinalis cardinalis) distributed across the Cochise Filter Barrier, a region of transitional habitat which separates the Sonoran and Chihuahuan deserts. Using genome-wide markers, we modeled demographic history by fitting the data to isolation and isolation-with-migration models. The best-fit model indicated that desert populations diverged in the mid-Pleistocene and there has been historically low, unidirectional gene flow into the Sonoran Desert. We then tested song recognition using reciprocal call-broadcast experiments to compare song recognition between deserts, controlling for song dialect changes within deserts. We found that male Northern Cardinals in both deserts were most aggressive to local songs and failed to recognize across-barrier songs. A correlation of genomic differentiation despite historic introgression and strong song discrimination is consistent with a model where speciation is initiated across a barrier and maintained by behavioral isolation.
Preservation of circadian rhythms by the protein folding chaperone, BiP
Authors: Pickard Adam , Chang Joan , Alachkar Nissrin , Calverley Ben , Garva Richa , Arvan Peter , Meng Qing-Jun , Kadler Karl E. 
Published: June 2018

AbstractER stress and dysregulation of collagen synthesis are associated with progression of disease in cancer and fibrosis. Collagen synthesis is co-ordinated with the circadian clock, which curiously in cancer cells, is deregulated by ER stress. We hypothesised that interplay exists between circadian rhythm, collagen synthesis and ER stress in normal cells. Here we show that fibroblasts with ER stress do not demonstrate circadian rhythms in gene expression upon clock-synchronizing time cues. Conversely, overexpression of BiP or treatment with chemical chaperones strengthens the oscillation amplitude of circadian rhythms. The significance of these findings was explored in tendon, where we showed that BiP expression is ramped preemptively prior to a surge in collagen synthesis at night, thereby preventing protein misfolding and ER stress. In turn, we propose, this forestalls activation of the unfolded protein response in order for circadian rhythms to be maintained. Thus, targeting ER stress could be used to modulate circadian rhythm and restore collagen homeostasis in disease.
WDR5 regulates epithelial-to-mesenchymal transition in breast cancer cellsviaTGFβ
Authors: Simona Punzi , Chiara Balestrieri , Carolina D’Alesio , Daniela Bossi , Ivan Dellino Gaetano , Elena Gatti , Giancarlo Pruneri , Carmen Criscitiello , Alessandro Carugo , Giuseppe Curigliano , Gioacchino Natoli , Giuseppe Pelicci Pier , Luisa Lanfrancone 
Published: June 2018

AbstractEven if the mortality rate in breast cancer (BC) has recently decreased, development of metastases and drug resistance are still challenges to successful systemic treatment. The epithelial-to-mesenchymal transition (EMT), as well as epigenetic dynamic modifications, plays a pivotal role in invasion, metastasis, and drug resistance. Here, we report that WDR5, the core subunit of histone H3 K4 methyltransferase complexes, is crucial in coordinating EMT and regulating epigenetic changes that drive metastasis. We show that silencing of WDR5 in BC up-regulates an epithelial signature in triple negative and luminal B like patients by transcriptional repression of mesenchymal genes and reduction of the metastatic properties of these cells. Moreover, we demonstrate that this regulation is mediated by inhibition of the TGFβ signaling both at the transcriptional and post-translational level, suggesting an active role of WDR5 in guiding tumor plasticity upon oncogenic insults, regardless of the pathological BC subtypes.We therefore suggest that WDR5 inhibition could be a successful pharmacologic approach to inhibit EMT and sensitize breast cancer cells to chemotherapy.
Dynamics of PAR proteins explain the oscillation and ratcheting mechanisms in dorsal closure
Authors: Durney C.H. , Harris T.J.C. , Feng J.J. 
Published: June 2018

AbstractWe present a vertex-based model forDrosophiladorsal closure that predicts the mechanics of cell oscillation and contraction from the dynamics of the PAR proteins. Based on experimental observations of how aPKC, Par-6 and Bazooka migrate from the circumference of the apical surface to the medial domain, and how they interact with each other and ultimately regulate the apicomedial actomyosin, we formulate a system of differential equations that capture the key features of the process. The oscillation in cell area in the early phase of dorsal closure results from an intracellular negative feedback loop that involves myosin, an actomyosin regulator, aPKC and Bazooka. In the slow phase, gradual sequestration of apicomedial aPKC into Bazooka clusters causes incomplete disassembly of the myosin network over each cycle of oscillation, thus producing the so-called ratchet. The fast phase of rapid cell and tissue contraction arises when medial myosin, no longer hindered by aPKC, builds up in time and produces sustained contraction. Thus, a minimal set of rules governing the dynamics of the PAR proteins, extracted from experimental observations, can account for all major mechanical outcomes of dorsal closure, including the transitions between its three distinct phases.Insert Received for publication Date and in final form Date
NADP-dependent malic enzyme 1 participates in the abscisic acid response in Arabidopsis thaliana
Authors: Arias Cintia L. , Pavlovic Tatiana , Torcolese Giuliana , Badia Mariana B. , Gismondi Mauro , Maurino Verónica G. , Andreo Carlos S. , Drincovich María F. , Gerrard Wheeler Mariel C. , Saigo Mariana 
Published: June 2018

AbstractArabidopsis thaliana possesses three cytosolic (NADP-ME1-3) and one plastidic (NADP-ME4) NADP-dependent malic enzymes. NADP-ME2 and-ME4 show constitutive expression, in contrast to NADP-ME1 and-ME3, which are restricted to particular tissues. Here, we show that NADP-ME1 transcript and protein were almost undetectable during normal vegetative growth, but gradually increased and reached levels higher than those of the other isoforms in the latest stages of seed development. Accordingly, in knockout nadp-me1 mature seeds the total NADP-ME activity was significantly lower than in wild type mature seeds. The phenotypic analysis of nadp-me1 plants indicated alterations of seed viability and germination. Besides, the treatment with abscisic acid (ABA), NaCl and mannitol specifically induced the accumulation of NADP-ME1 in seedlings. In line with this, nadp-me1 plants show a weaker response of primary and lateral root length and stomatal opening to the presence of ABA.The results suggest that NADP-ME1 plays a specialized role, linked to ABA signalling during the seed development as well as in the response to saline and osmotic stress.
Relationship between impulsivity, uncontrolled eating and body mass index: a hierarchical model
Authors: Garcia-Garcia Isabel , Neseliler Selin , Morys Filip , Dadar Mahsa , Yau Yvonne H.C. , Scala Stephanie G. , Zeighami Yashar , Sun Natalie , Collins D. Louis , Vainik Uku , Dagher Alain 
Published: June 2018

AbstractBackgroundImpulsivity increases the risk for obesity and weight gain. However, the precise role of impulsivity in the aetiology of overeating behavior and obesity is currently unknown. Here we examined the relationships between personality-related measures of impulsivity, Uncontrolled Eating, BMI, and longitudinal weight changes. Additionally, we analyzed the associations between general impulsivity domains and brain cortical thickness to elucidate brain vulnerability factors related to weight gain.MethodsStudents in their first year of university - a risky period for weight gain - completed questionnaire measures of impulsivity and eating behavior at the beginning (N = 2318) of the school year. We also collected their weight at the end of the term (N = 1197). Impulsivity was divided into factors stress reactivity, reward sensitivity and lack of self-control. Using structural equation models, we tested the plausibility of a hierarchical relationship, in which impulsivity traits were associated with Uncontrolled Eating, which in turn predicted BMI and weight change. 71 participants underwent T1-weighted MRI to investigate the correlation between impulsivity and cortical thickness.ResultsImpulsivity traits showed positive correlations with Uncontrolled Eating. Higher scores in Uncontrolled Eating were in turn associated with higher BMI. None of the impulsivity-related measurements nor Uncontrolled Eating were correlated with longitudinal weight gain. Higher stress sensitivity was associated with increased cortical thickness in the superior temporal gyrus. Lack of self-control was positively associated with increased thickness in the superior medial frontal gyrus. Finally, higher reward sensitivity was associated with lower thickness in the inferior frontal gyrus.ConclusionThe present study provides a comprehensive characterization of the relationships between different facets of impulsivity and obesity. We show that differences in impulsivity domains might be associated with BMI via Uncontrolled Eating. Our results might inform future clinical strategies aimed at fostering self-control abilities to prevent and/or treat unhealthy weight gain.
The N-cadherin interactome in primary cardiomyocytes as defined by quantitative proximity proteomics
Authors: Li Yang , Merkel Chelsea D. , Zeng Xuemei , Heier Jonathon A. , Cantrell Pamela S. , Sun Mai , Stolz Donna B. , Watkins Simon C. , Yates Nathan A. , Kwiatkowski Adam V. 
Published: June 2018

AbstractThe junctional complexes that couple cardiomyocytes must transmit the mechanical forces of contraction while maintaining adhesive homeostasis. The adherens junction (AJ) connects the actomyosin networks of neighboring cardiomyocytes and is required for proper heart function. Yet little is known about the molecular composition of the cardiomyocyte AJ or how it is organized to function under mechanical load. Here we define the architecture, dynamics and proteome of the cardiomyocyte AJ. Mouse neonatal cardiomyocytes assemble stable AJs along intercellular contacts with organizational and structural hallmarks similar to mature contacts. We combine quantitative mass spectrometry with proximity labeling to identify the N-cadherin (CDH2) interactome. We define over 350 proteins in this interactome, nearly 200 of which are unique to CDH2 and not part of the E-cadherin (CDH1) interactome. CDH2-specific interactors are comprised primarily of adaptor and adhesion proteins that promote junction specialization. Finally, we find evidence of dynamic interplay between AJ and Z-disc proteins. Together, our results provide novel insight into the cardiomyocyte AJ and provide a proteomic atlas for defining the molecular complexes that regulate cardiomyocyte intercellular adhesion.Summary StatementProximity proteomics reveals a specific and specialized N-cadherin (CDH2) interactome along the cell-cell contacts of primary cardiomyocytes.
Carleson measure and Volterra type operators on weighted BMOA spaces
Authors: Qian Ruishen , Li Songxiao 
Published: June 2018

Abstract
Let μ be a nonnegative Borel measure on the unit disk

𝔻

{\mathbb{D}}

. In this paper, we investigate measures μ such that the weighted

BMOA

{\mathrm{BMOA}}

spaces are embedded boundedly or compactly into tent-type spaces

𝒯
φ
∞

⁢

(
μ
)

{\mathcal{T}_{\varphi}^{\infty}(\mu)}

. As an application, we characterize the boundedness and compactness of Volterra integral operators on the weighted

BMOA

{\mathrm{BMOA}}

space.
›Wirklichkeitsverlust‹, ›Wirklichkeitshunger‹ und »Neuer Realismus«
Authors: Gittel Benjamin 
Published: June 2018

AbstractLiterary Studies of contemporary works of literature, in the absence of a canon, are largely directed by order-making umbrella terms. Using the umbrella term ‘new realism’ as an example this study examines (1) its relationship with poetological discourses and discourses of a diagnosis of the present, (2) how the umbrella term’s attractiveness corresponds to its conceptual underdetermination, and (3) which different forms text analytical concepts of ‘new realism’ can take.
Die edition suhrkamp
Authors: Michalski Claudia 
Published: June 2018

AbstractThe article focuses on two important paratextual features of edition suhrkamp – its cover design and its seriality. In examining the complex relationship of these features both with respect to each other and to the image and content of edition suhrkamp, it can be shown that the paratext produces a surplus of meaning and thus allows the series to represent both the publishing firm of Suhrkamp and the “Suhrkamp Culture”.
Influence of disinfectant «Bi-des» on the sporulation on eimery oocyst of chicken
Authors: Golubtsova M.V. , Stybel V.V. , Sobolta A.G. 
Published: June 2018

Eymeriosis (coccidiosis) is a protozoal disease of a poultry characterized by inhibition, loss of appetite, thirst, diarrhea, often with blood, anemia, nervous phenomena. Diseases are registered in the world everywhere, including in Ukraine. Regardless of its type eymeriosis is one of the most serious technological diseases of farm birds in developed countries. Sick animals excreted with oocysts, which are the source of invasion, and contaminated by them feed, water, litter, equipment of livestock buildings, objects of care, etc. are the factors of transmission. Infection with coccidia occurs in an elementary way by swallowing an oocyst with water or food. The results of the study of the des-invasive effect of the Bi-des preparation on oocysts of chicken eimery are presented. In the formulation of the proposed disinfectant, as ADR included: PGMG-hydrochloride and triamine and auxiliaries substances – cocamidopropyl-betaine and glutamic acid as well. Due to which this pharmaceutical combination has a cleansing effect. Researches of the effect of the «Bi-des» preparation on the process of sporulation of the oocyst E. tenella was carried out at concentrations of 1, 2 and 3% with exposition of one, two, three and four hours. It was found that the preparation of 1% concentration with exposition of three and four hours caused the death of almost half of the oocyst eimery (44.98 and 47.9%). Sufficiently high oocystocidal effect (62.7–79.9%) were found at 2% disinfectant concentration with different exposition regimes. 3 hours exposition caused 100% lysis oocyst eimeria of chicken. It was established that using the preparation caused visible destructive changes of morphology of the simplest and led to the static effect on the sporulation of the oocyst.
They Don’t Care About Us! Care Personnel’s Perspectives on Ambient Assisted Living Technology Usage: Scenario-Based Survey Study
Authors: Offermann-van Heek Julia , Ziefle Martina 
Published: June 2018

Background
Demographic change represents enormous burdens for the care sectors, resulting in high proportions of (older) people in need of care and a lack of care staff. Ambient Assisted Living (AAL) technologies have the potential to support the bottlenecks in care supply but are not yet in widespread use in professional care contexts.

Objective
The objective of our study was to investigate professional caregivers’ AAL technology acceptance and their perception regarding specific technologies, data handling, perceived benefits, and barriers. In particular, this study focuses on the perspectives on AAL technologies differing between care professionals working in diverse care contexts to examine the extent to which the care context influences the acceptance of assistive technologies.

Methods
A Web-based survey (N=170) was carried out focusing on professional caregivers including medical, geriatric, and disabled people’s caregivers. Based on a scenario, the participants were asked for their perceptions concerning specific technologies, specific types of gathered data, and potential benefits of and barriers to AAL technology usage.

Results
The care context significantly impacted the evaluations of AAL technologies (F14,220=2.514; P=.002). Professional caregivers of disabled people had a significantly more critical attitude toward AAL technologies than medical and geriatric caregivers, indicated (1) by being the only caregiver group that rejected evaluations of AAL technology acceptance (F2,118=4.570; P=.01) and specific technologies (F2,118=11.727; P<.001) applied for gathering data and (2) by the comparatively lowest agreements referring to the evaluations of data types (F2,118=4.073, P=.02) that are allowed to be gathered.

Conclusions
AAL technology acceptance is critical because of technology implementation reasons, especially in the care of people with disabilities. AAL technologies in care contexts have to be tailored to care professional’s needs and concerns (“care about us”). The results contribute to a broader understanding of professional caregivers’ needs referring to specific data and technology configurations and enclose major differences concerning diverse care contexts. Integrating these findings into user group-tailored technology concepts and communication strategies will support a sustainable adoption of AAL systems in professional care contexts.