bluuebunny/crossref_metadata_2025_split huggingface dataset:
Balance design for robust foliar nutrient diagnosis of “Prata” banana (Musa spp.)
Authors: de Deus José Aridiano Lima , Neves Júlio César Lima , Corrêa Márcio Cleber de Medeiros , Parent Serge-Étienne , Natale William , Parent Léon Etienne 
Published: October 2018

AbstractThe “Cavendish” and “Prata” subgroups represent respectively 47% and 24% of the world banana production. Compared to world average progressing from 10.6 to 20.6 t ha−1between 1961 and 2016, and despite sustained domestic demand and the introduction of new cultivars, banana yield in Brazil has stagnated around 14.5 t ha−1mainly due to nutrient and water mismanagement. “Prata” is now the dominant subgroup in N-E Brazil and is fertigated at high costs. Nutrient balances computed as isometric log-ratios (ilr) provide a comprehensive understanding of nutrient relationships in the diagnostic leaf at high yield level by combining raw concentration data. Although the most appropriate method for multivariate analysis of compositional balances may be less efficient due to non-normal data distribution and limited nutrient mobility in the plant, robustness of the nutrient balance approach could be improved using Box-Cox exponents assigned to raw foliar concentrations. Our objective was to evaluate the accuracy of nutrient balances to diagnose fertigated “Prata” orchards. The dataset comprised 609 observations on fruit yields and leaf tissue compositions collected from 2010 to 2016 in Ceará state, N-E Brazil. Raw nutrient concentration ranges were ineffective as diagnostic tool due to considerable overlapping of concentration ranges for low- and high-yielding subpopulations at cutoff yield of 40 Mg ha−1. Nutrient concentrations were combined into isometric log-ratios (ilr) and normalized by Box-Cox corrections between 0 and 1 which may also account for restricted nutrient transfer from leaf to fruit. Despite reducedilrskewness, Box-Cox coefficients did not improve model robustness measured as the accuracy of the Cate-Nelson partition between yield and the multivariate distance acrossilrvalues. Sensitivity was 94%, indicating that low yields are attributable primarily to nutrient imbalance. There were 148 false-positive specimens (high yield despite nutrient imbalance) likely due to suboptimal nutrition, contamination, or luxury consumption. The profitability of “Prata” orchards could be enhanced by rebalancing nutrients usingilrstandards with no need for Box-Cox correction.
Versatile chemical handling to confine radioactive cesium as stable inorganic crystal
Authors: Duy Quang Nguyen , Eba Hiromi , Sakurai Kenji 
Published: October 2018

AbstractThe present paper describes an extremely efficient, reproducible and inexpensive chemical handling method for converting the nuclear wastes contaminated by radioactive cesium to stable inorganic crystal, pollucite (CsAlSi2O6), which is promising as a form of the final storage. In this processing, the clays are used as a source for aluminum and silicon, and it is important to get a well-mixed homogenous solution by the aid of some heat and pressure. The present method proposes the use of ethylene glycol as a solvent, rather than water. It has been found that one can obtain crystalline pollucite by heating up to 350 °C in a high-pressure container (~15 MPa), mixed with montmorillonite – an abundant natural clay and ethylene glycol. It has been found that the reduction of the amount of water helps to achieve very high confinement rate in a reasonable time of few~20 h. This will be fairly important in processing contaminated water in the nuclear power plant. The influence of seawater has been also examined.
Robust Enhancement of Lentivirus Production by Promoter Activation
Authors: Suzuki Naoto , Yoshida Takeshi , Takeuchi Hiroaki , Sakuma Ryuta , Sukegawa Sayaka , Yamaoka Shoji 
Published: October 2018

AbstractLentiviral vectors are a valuable tool to deliver exogenous genes for stable expression in cells. While much progress has been made in processing lentiviral vector-containing culture medium, it remains to be explored how the production of lentiviral vector from producer cells can be increased. We initially found that co-expression of the SPRY domain-containing SOCS box protein 1 (SPSB1) promotes the production of human immunodeficiency virus type 1 (HIV-1) and lentiviral vector with increased expression of the Gag and envelope proteins and activation of the HIV-1 LTR and CMV promoter. The presence of AP-1, NF-κB and CREB/ATF recognition sites in these promoters prompted us to utilize human T-lymphotropic virus type 1 (HTLV-1) Tax for lentiviral vector production because Tax activates all these transcription factors. Co-expression of a small amount of Tax markedly increased both the expression of viral structural proteins in producer cells and release of lentiviral vector particles, resulting in a more than 10-fold enhancement of transduction efficiency. Of note, the Tax protein was not detected in the lentiviral vector particles concentrated by ultracentrifugation, supporting the safety of this preparation. Collectively, these results indicate that promoter activation in producer cells represents a promising approach to preparing high-titer lentiviral vectors.
Higher species richness enhances yield stability in intensively managed grasslands with experimental disturbance
Authors: Haughey Eamon , Suter Matthias , Hofer Daniel , Hoekstra Nyncke J. , McElwain Jennifer C. , Lüscher Andreas , Finn John A. 
Published: October 2018

AbstractClimate models predict increased frequency and severity of drought events. At an Irish and Swiss site, experimental summer droughts were applied over two successive years to grassland plots sown with one, two or four grassland species with contrasting functional traits. Mean yield and plot-to-plot variance of yield were measured across harvests during drought and after a subsequent post-drought recovery period. At both sites, there was a positive relationship between species richness and yield. Under rainfed control conditions, mean yields of four-species communities were 32% (Wexford, Ireland) and 51% (Zürich, Switzerland) higher than in monocultures. This positive relationship was also evident under drought, despite significant average yield reductions (−27% at Wexford; −21% at Zürich). Four-species communities had lower plot-to-plot variance of yield compared to monoculture or two-species communities under both rainfed and drought conditions, which demonstrates higher yield stability in four-species communities. At the Swiss but not the Irish site, a high degree of species asynchrony could be identified as a mechanism underlying increased temporal stability in four-species communities. These results indicate the high potential of multi-species grasslands as an adaptation strategy against drought events and help achieve sustainable intensification under both unperturbed and perturbed environmental conditions.
A frequency reconfigurable dipole antenna with solid-state plasma in silicon
Authors: Kim Da-Jin , Jo Eon-Seok , Cho Young-Kyun , Hur Jae , Kim Choong-Ki , Kim Cheol Ho , Park Bonghyuk , Kim Dongho , Choi Yang-Kyu 
Published: October 2018

AbstractA frequency reconfigurable dipole antenna based on a silicon radiator is presented. The silicon radiator is activated with the aid of highly dense solid-state plasma by injecting carriers into the intrinsic region of p-i-n diodes. The fabrication and design guideline of the reconfigurable dipole antenna with this plasma radiator are described. When the plasma radiator is activated or deactivated, the length of the dipole arm changes, which means that the operating frequency of the dipole antenna is reconfigurable. When all the channels in the plasma radiator are activated, the operating frequency is tuned from 6.3 GHz to 4.9 GHz. The measured tunable bandwidth of our fabricated dipole antenna is approximately 31%, which is a practical value in comparison to conventional frequency reconfigurable antennas whose tunable bandwidth is in a range from 20% to 50%. To further support the validity of our results, we provide the well-matched simulation results from an antenna simulation. These results demonstrate that silicon with its commercial technology, which has not attracted attention in comparison to a metal antennas, is a promising tunable material for a frequency reconfigurable antenna. This plasma-based reconfigurable antenna has great potential for use in the dynamic communication environment.
Multiplex Biomarker Screening Assay for Urinary Extracellular Vesicles Study: A Targeted Label-Free Proteomic Approach
Authors: Chutipongtanate Somchai , Greis Kenneth D. 
Published: October 2018

AbstractThe recent advance in targeted label-free proteomics, SWATH-MS, can provide consistent protein detection and reproducible protein quantitation, which is a considerable advantage for biomarker study of urinary extracellular vesicles. We developed a SWATH-MS workflow with a curated spectral library of 1,145 targets. Application of the workflow across nine replicates of three sample types (exosome-like vesicles (ELVs), microvesicles (MVs) and urine proteins (UPs)) resulted in the quantitation of 888 proteins at FDR <1%. The median-coefficient of variation of the 888 proteins in the ELV sample was 7.7%, indicating excellent reproducibility. Data analysis showed common exosome markers, (i.e. CD9, CD63, ALIX, TSG101 and HSP70) were enriched in urinary ELVs as compared to MVs and UPs. The use of a multiplex biomarker screening assay focused on ELVs was investigated, and perspectives in future applications are discussed.
Comparison Of Four Dietary Scores As Determinants Of Coronary Heart Disease Mortality
Authors: Menotti Alessandro , Puddu Paolo Emilio 
Published: October 2018

AbstractWe aimed at comparing 2 a-priori -Mediterranean Adequacy Index (MAI), the Median Score (MED) - versus 2 a-posteriori, -Factor Analysis (FA2) and Principal Components analysis (PC2)- dietary scores in 1214 CHD-free men aged 45–64 belonging to the Italian Rural Areas of the Seven Countries Study examined in 1965 and followed-up for mortality during 40 years. CHD death was the end-point. Collection of dietary history allowed to define 17 main food groups expressed in gr/day and to compare dietary scores, each divided into 3 classes. Kaplan-Meier curves showed higher survival for classes 2 and 3 (healthy) versus class 1 (unhealthy), but the log-rank test was not significant for the 2 a-priori scores. Cox proportional hazards models showed similar significant findings comparing class 3 with class 1 in the a-posteriori scores FA2 and PC2, with hazards ratios of 0.48 and 0.43, that became 0.65 and 0.53 respectively after adjusting for six specific risk factors for CHD (age, cigarette smoking, systolic blood pressure, serum cholesterol, body mass index and physical activity). Food intake of class 3 in all 4 scores matched rather well the characteristics of the Mediterranean Diet. The poor performance of a-priori dietary scores was partly explained by the unexpected and direct relationship of fruit intake with CHD deaths. The superiority of a-posteriori dietary patterns could be in turn due to the specificity of the study population. External validations and comparisons of a-priori versus a-posteriori dietary patterns in larger cohorts, using the same 17 food groups explored here, are urgently needed.
An innovative flow cytometry method to screen human scFv-phages selected by in vivo phage-display in an animal model of atherosclerosis
Authors: Hemadou Audrey , Laroche-Traineau Jeanny , Antoine Ségolène , Mondon Philippe , Fontayne Alexandre , Le Priol Yannick , Claverol Stéphane , Sanchez Stéphane , Cerutti Martine , Ottones Florence , Clofent-Sanchez Gisèle , Jacobin-Valat Marie-Josée 
Published: October 2018

AbstractAtherosclerosis is a chronic, progressive inflammatory disease that may develop into vulnerable lesions leading to thrombosis. This pathology is characterized by the deposition of lipids within the arterial wall and infiltration of immune cells leading to amplification of inflammation. Nowadays there is a rising interest to assess directly the molecular and cellular components that underlie the clinical condition of stroke and myocardial infarction. Single chain fragment variable (scFv)-phages issuing from a human combinatorial library were selected on the lesions induced in a rabbit model of atherosclerosis after three rounds of in vivo phage display. We further implemented a high-throughput flow cytometry method on rabbit protein extracts to individually test one thousand of scFv-phages. Two hundred and nine clones were retrieved on the basis of their specificity for atherosclerotic proteins. Immunohistochemistry assays confirmed the robustness of the designed cytometry protocol. Sequencing of candidates demonstrated their high diversity in VH and VL germline usage. The large number of candidates and their diversity open the way in the discovery of new biomarkers. Here, we successfully showed the capacity of combining in vivo phage display and high-throughput cytometry strategies to give new insights in in vivo targetable up-regulated biomarkers in atherosclerosis.
Efficient and Sensitive Electrically Small Rectenna for Ultra-Low Power RF Energy Harvesting
Authors: Assimonis Stylianos D. , Fusco Vincent , Georgiadis Apostolos , Samaras Theodoros 
Published: October 2018

AbstractA new electrically small antenna with size ka = 0.415 is presented, fabricated and measured in this work. This is intrinsically matched to 50 Ω, has omni-directional and linear-polarized radiation pattern in the horizontal plane with maximum directivity of 1.75 dBi and simulated radiation efficiency of 93%. The antenna in combination with a low-complex and co-planar rectifier with one single diode forms a high efficient and sensitive electrically small rectenna with ka = 0.53 at 868 MHz (UHF RFID-band in Europe). The latter has measured efficiency 22.5% for −19 dBm power input and sensitivity of −44 dBm (or equivalently 0.00028 μW/cm2 power density), while at 2.25 μW/cm2 is able to supply continuously, i.e., without a boost converter or use of any energy tank, a small electrical device with 118 μW. In order to increase the dc output voltage and the delivered dc power to the load for lower power density levels, rectenna-array configuration is exploited. Application to batteryless, backscatter wireless sensor node powering is discussed. Specifically, for a power density of 0.1237 μW/cm2 the RF energy harvesting system delivers 172 μW at 2.85 V every 22.5 s.
Structural Rearrangements Maintain the Glycan Shield of an HIV-1 Envelope Trimer After the Loss of a Glycan
Authors: Ferreira Roux-Cil , Grant Oliver C. , Moyo Thandeka , Dorfman Jeffrey R. , Woods Robert J. , Travers Simon A. , Wood Natasha T. 
Published: October 2018

AbstractThe HIV-1 envelope (Env) glycoprotein is the primary target of the humoral immune response and a critical vaccine candidate. However, Env is densely glycosylated and thereby substantially protected from neutralisation. Importantly, glycan N301 shields V3 loop and CD4 binding site epitopes from neutralising antibodies. Here, we use molecular dynamics techniques to evaluate the structural rearrangements that maintain the protective qualities of the glycan shield after the loss of glycan N301. We examined a naturally occurring subtype C isolate and its N301A mutant; the mutant not only remained protected against neutralising antibodies targeting underlying epitopes, but also exhibited an increased resistance to the VRC01 class of broadly neutralising antibodies. Analysis of this mutant revealed several glycans that were responsible, independently or through synergy, for the neutralisation resistance of the mutant. These data provide detailed insight into the glycan shield’s ability to compensate for the loss of a glycan, as well as the cascade of glycan movements on a protomer, starting at the point mutation, that affects the integrity of an antibody epitope located at the edge of the diminishing effect. These results present key, previously overlooked, considerations for HIV-1 Env glycan research and related vaccine studies.
Raman spectroscopic detection of high-grade cervical cytology: Using morphologically normal appearing cells
Authors: Duraipandian Shiyamala , Traynor Damien , Kearney Padraig , Martin Cara , O’Leary John J. , Lyng Fiona M. 
Published: October 2018

AbstractThis study aims to detect high grade squamous intraepithelial cells (HSIL) by investigating HSIL associated biochemical changes in morphologically normal appearing intermediate and superficial cells using Raman spectroscopy. Raman spectra (n = 755) were measured from intermediate and superficial cells from negative cytology ThinPrep specimens (n = 18) and from morphologically normal appearing intermediate and superficial cells from HSIL cytology ThinPrep specimens (n = 17). The Raman data was subjected to multivariate algorithms including the standard principal component analysis (PCA)-linear discriminant analysis (LDA) and partial least squares discriminant analysis (PLS-DA) together with random subsets cross-validation for discriminating negative cytology from HSIL. The PCA-LDA method yielded sensitivities of 74.9%, 72.8%, and 75.6% and specificities of 89.9%, 81.9%, and 84.5%, for HSIL diagnosis based on the dataset obtained from intermediate, superficial and mixed intermediate/superficial cells, respectively. The PLS-DA method provided improved sensitivities of 95.5%, 95.2% and 96.1% and specificities of 92.7%, 94.7% and 93.5% compared to the PCA-LDA method. The results demonstrate that the biochemical signatures of morphologically normal appearing cells can be used to discriminate between negative and HSIL cytology. In addition, it was found that mixed intermediate and superficial cells could be used for HSIL diagnosis as the biochemical differences between negative and HSIL cytology were greater than the biochemical differences between intermediate and superficial cell types.
Analysis of application effect of 220 kV UPFC demonstration project in Shanghai grid
Authors: Cui Yong , Yu Yinghui , Bao Wei , Feng Yuyao , Guo Qiang , Xie Wei , Jin Minjie 
Published: October 2018

The feasibility study of unified power flow controller (UPFC) application in 220 kV Shanghai Grid is conducted based on the analysis of the practical operation of the grid. The location selection method and scheme are proposed for the application of 220 kV UPFC demonstration project. The application effect of the demonstration project of UPFC is evaluated from various perspectives including enhancement of interface transfer capability, improvement of voltage stability performance of the grid and contribution to balancing of reactive power in 220 kV sectionalised systems.
Development of online monitor for substation 10 kV MOA
Authors: Song Xinming , Xia Yunfeng , He Jianzong , Jia Zhidong , Wang Xilin 
Published: October 2018

Metal oxide arresters (MOA) are widely used for over‐voltage protection of power facilities in 10 kV distribution networks, and it is hard to determine the failure ones. This study presented an online monitor for 10 kV MOA used in substation. Firstly, with simulation on electromagnetic transient program (EMTP), the authors found that the resistive component of leakage current flowing through the MOA could reflect its health status. Then the principle of detecting the resistive leakage current flowing MOA by the harmonic analysis method is introduced. Finally, a remote monitoring system for MOA based on wireless communication technology is built and testing experiments of monitoring the health status of MOA has been done.
Detection of a high-mass prestellar core candidate in W43-MM1
Authors: Nony T. , Louvet F. , Motte F. , Molet J. , Marsh K. , Chapillon E. , Gusdorf A. , Brouillet N. , Bontemps S. , Csengeri T. , Despois D. , Nguyen Luong Q. , Duarte-Cabral A. , Maury A. 
Published: October 2018

Aims. To constrain the physical processes that lead to the birth of high-mass stars it is mandatory to study the very first stages of their formation. We search for high-mass analogs of low-mass prestellar cores in W43-MM1.
Methods. We conducted a 1.3 mm ALMA mosaic of the complete W43-MM1 cloud, which has revealed numerous cores with ~2000 au FWHM sizes. We investigated the nature of cores located at the tip of the main filament, where the clustering is minimum. We used the continuum emission to measure the core masses and the 13CS(5-4) line emission to estimate their turbulence level. We also investigated the prestellar or protostellar nature of these cores by searching for outflow signatures traced by CO(2-1) and SiO(5-4) line emission, and for molecular complexity typical of embedded hot cores.
Results. Two high-mass cores of ~1300 au diameter and ~60 M⊙ mass are observed to be turbulent but gravitationally bound. One drives outflows and is associated with a hot core. The other core, W43-MM1#6, does not yet reveal any star formation activity and thus is an excellent high-mass prestellar core candidate.
Assessment and Management of Over-Activity and Sleep Disorder in Mucopolysaccharidoses
Authors: Hare Dougal , Mahon Louise , Rust Stewart 
Published: October 2018

AbstractThere is a growing awareness, based on both research and clinical studies, that abnormal sleep and circadian functioning are associated with the various forms of mucopolysaccharidoses (MPS), with sleep respiratory problems seemingly common in many forms of MPS. In the case of MPS III, there is evidence of syndrome-specific sleep disturbance related to an abnormal and possibly unique circadian rhythm. The presence of such patterns of sleep and activity has detrimental consequences for both the person with MPS and also invariably for her/his family and carers. On the basis of current evidence, routine assessment of sleep functioning is clearly warranted for all children with MPS III together with appropriate medical and behavioral interventions as required.
Risk of Cesarean Delivery among Nulliparous Asian-Indian Women with Term Singleton Pregnancies
Authors: Melamed Alexander , Bryant Allison , Prabhu Malavika 
Published: October 2018

Objective Asian-Indian women are a growing population in the United States, but little data exist about their risk of cesarean delivery (CD). We characterize the odds of CD among Asian-Indian women and determine whether neonatal birth weight modifies this relationship.
Study Design This is a retrospective cohort study using an administrative perinatal database from California. We identified 1,029,940 nulliparous women with live, singleton, nonanomalous deliveries between 37 and 42 completed weeks of gestation. We performed multivariable logistic regression analyses to determine if Asian-Indian women were more likely to deliver by CD, compared with white non-Hispanic women, adjusting for sociodemographic and clinical variables. We explored if birth weight was an effect modifier, testing the interaction term's significance using Wald's test, and performed multivariable logistic regressions stratified by birth weight category.
Results Asian-Indian women comprised 2.0% of the cohort. Compared with white non-Hispanic women, Asian-Indian women had an adjusted odds of 1.41 (95% confidence interval: 1.36–1.46) for CD. However, we noted effect modification of birth weight on the odds of CD by race/ethnicity (p < 0.001). Among all birth weight categories exceeding 3,000 g, Asian-Indian women had higher odds of CD than white non-Hispanic women.
Conclusion Asian-Indian women are at greater risk of CD than white non-Hispanic women when birthweight exceeds 3,000 g.