bluuebunny/crossref_metadata_2025_split huggingface dataset:
Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors
Authors: Guo Rongqun , Hu Fangxiao , Weng Qitong , Lv Cui , Wu Hongling , Liu Lijuan , Li Zongcheng , Zeng Yang , Bai Zhijie , Zhang Mengyun , Liu Yuting , Liu Xiaofei , Xia Chengxiang , Wang Tongjie , Zhou Peiqing , Wang Kaitao , Dong Yong , Luo Yuxuan , Zhang Xiangzhong , Guan Yuxian , Geng Yang , Du Juan , Li Yangqiu , Lan Yu , Chen Jiekai , Liu Bing , Wang Jinyong 
Published: June 2019

ABSTRACTAchievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells is a central aim in T cell regenerative medicine. To date, preferentially regenerating T lymphopoiesis in vivo from pluripotent stem cells (PSC) remains a practical challenge. Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial to hematopoietic transition and hematopoietic maturation stages induced in vitro from PSC (iR9-PSC) preferentially generated engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T (iT) cells in primary and secondary immunodeficient recipients. Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSC, unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors. The iT cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβ repertoire. The regenerative T lymphopoiesis rescued the immune-surveillance ability in immunodeficient mice. Furthermore, gene-edited iR9-PSC produced tumor-specific-T cells in vivo that effectively eradicated tumor cells. This study provides insight into universal generation of functional and therapeutic T lymphopoiesis from the unlimited and editable PSC source.
Inferring biosynthetic and gene regulatory networks from Artemisia annua RNA sequencing data on a credit card-sized ARM computer
Authors: Tan Qiao Wen , Mutwil Marek 
Published: June 2019

0.ABSTRACTPrediction of gene function and gene regulatory networks is one of the most active topics in bioinformatics. The accumulation of publicly available gene expression data for hundreds of plant species, together with advances in bioinformatical methods and affordable computing, sets ingenuity as the major bottleneck in understanding gene function and regulation. Here, we show how a credit card-sized computer retailing for less than 50 USD can be used to rapidly predict gene function and infer regulatory networks from RNA sequencing data. To achieve this, we constructed a bioinformatical pipeline that downloads and allows quality-control of RNA sequencing data; and generates a gene co-expression network that can reveal enzymes and transcription factors participating and controlling a given biosynthetic pathway. We exemplify this by first identifying genes and transcription factors involved in the biosynthesis of secondary cell wall in the plant Artemisia annua, the main natural source of the anti-malarial drug artemisinin. Networks were then used to dissect the artemisinin biosynthesis pathway, which suggest potential transcription factors regulating artemisinin biosynthesis. We provide the source code of our pipeline and envision that the ubiquity of affordable computing, availability of biological data and increased bioinformatical training of biologists will transform the field of bioinformatics.HighlightsProcessing of large scale transcriptomic data with affordable single-board computersTranscription factors can be found in the same network as their targetsCo-expression of transcription factors and genes in secondary cell wall biosynthesisCo-expression of transcription factors and genes involved in artemisinin biosynthesis
Decoding Speech and Music Stimuli from the Frequency Following Response
Authors: Losorelli Steven , Kaneshiro Blair , Musacchia Gabriella A. , Blevins Nikolas H. , Fitzgerald Matthew B. 
Published: June 2019

AbstractThe ability to differentiate complex sounds is essential for communication. Here, we propose using a machine-learning approach, called classification, to objectively evaluate auditory perception. In this study, we recorded frequency following responses (FFRs) from 13 normal-hearing adult participants to six short music and speech stimuli sharing similar fundamental frequencies but varying in overall spectral and temporal characteristics. Each participant completed a perceptual identification test using the same stimuli. We used linear discriminant analysis to classify FFRs. Results showed statistically significant FFR classification accuracies using both the full response epoch in the time domain (72.3% accuracy, p < 0.001) as well as real and imaginary Fourier coefficients up to 1 kHz (74.6%, p < 0.001). We classified decomposed versions of the responses in order to examine which response features contributed to successful decoding. Classifier accuracies using Fourier magnitude and phase alone in the same frequency range were lower but still significant (58.2% and 41.3% respectively, p < 0.001). Classification of overlapping 20-msec subsets of the FFR in the time domain similarly produced reduced but significant accuracies (42.3%–62.8%, p < 0.001). Participants’ mean perceptual responses were most accurate (90.6%, p < 0.001). Confusion matrices from FFR classifications and perceptual responses were converted to distance matrices and visualized as dendrograms. FFR classifications and perceptual responses demonstrate similar patterns of confusion across the stimuli. Our results demonstrate that classification can differentiate auditory stimuli from FFR responses with high accuracy. Moreover, the reduced accuracies obtained when the FFR is decomposed in the time and frequency domains suggest that different response features contribute complementary information, similar to how the human auditory system is thought to rely on both timing and frequency information to accurately process sound. Taken together, these results suggest that FFR classification is a promising approach for objective assessment of auditory perception.
Challenges of turboprop engine installation on small aircraft
Authors: Guła Paweł , Ulma Dawid , Żurek Krzysztof , Żurawski Rafał 
Published: June 2019

Purpose
The purpose of this paper is to present the challenges of turbine engine installation on small aircraft. The work was a part of the European Union project Efficient Systems and Propulsion for Small Aircraft, FP7 EU – Activity, 7.1.4. improving cost efficiency.

Design/methodology/approach
Few of the most interesting issues associated with replacing a piston engine with a turboprop engine were chosen: changes in engine bay cooling, air inlet, exhaust system, nacellès weight and parts reduction, flight tests and performance. The publication presents an approach to: design, assemble and test the small aircraft with a turboprop engine.

Findings
Replacement of piston engine by turbine was carried out. The full program of ground and flight test small aircraft has been successfully completed. Pros and cons of the new design are described in the paper.

Practical implications
Currently, aviation gasoline (AVGAS ) is increasingly being replaced by JET-A1 (kerosene-type fuels) or diesel fuel. The change concerns engine replacement and all the necessary additional components on the aircraft. This is consistent with the new directions of development of aviation: clean, green and eco design. Replacing the piston engine with a turbine allows improvement to performance and reduces operation cost.

Originality/value
The achieved results allow for identifying and highlighting new directions of aviation technology development. A significant added value is to draw attention to the necessity of preparing for future requirements and amendments in regulations for the new class of aircraft: general aviation SET(L) – single engine turboprop.
Macroeconomic factors and stock prices in the food and drink industry
Authors: Šimáková Jana , Stavárek Daniel , Pražák Tomáš , Ligocká Marie 
Published: June 2019

PurposeThe purpose of this paper is to estimate and evaluate the impact of macroeconomic fundamentals on stock prices of selected food and drink industry stocks during the period of 2005–2015, which saw the global financial crisis and its aftermath.Design/methodology/approachThe paper employed correlation analysis and the Johansen cointegration test with the vector error correction mechanism for EU companies operating in the food and drink industry. The paper tested the effects of GDP, inflation and interest rates (IR) on the stock prices of companies from Austria, Croatia, Cyprus, Denmark, Finland, Germany, Ireland, Italy, Lithuania, Poland, Spain and the UK.FindingsBased on the results, the authors can see that GDP has a generally positive effect on stock price development. In contrast, the relationship between stock prices and inflation and IR is negative in most cases.Originality/valueDespite the fact that a majority of empirical research on companies in the food and drink sector was performed using the microeconomic approach, this paper used the macroeconomic approach and clearly demonstrated the effects of selected macro-variables on stock prices in selected EU markets. Macroeconomic factors shape the company’s performance and could potentially lead to persistent changes in supply and demand conditions in food and drink markets.
The influence of abusive supervision on employees’ motivation and extra-role behaviors
Authors: Huang Liang-Chih , Su Chun-Hui , Lin Cheng-Chen , Lu Szu-Chi 
Published: June 2019

Purpose
The purpose of this paper is to attempt to unlock how and why abusive supervision influences employees’ day-to-day behaviors. Thus, the present study proposes that employees who are continuously faced with a supervisor’s hostile verbal and nonverbal behavior might obstruct their willingness to exhibit two different kinds of extra-role behaviors [i.e. organizational citizenship behavior (OCB) and voice] because sustained abusive behavior might hinder employees from their tasks and result in disengagement. Abused employees are more likely to disengage from their current tasks, and this is likely to in turn result in lower OCB and voice.

Design/methodology/approach
The data were collected from a Taiwan mid-sized high-tech manufacturing company. The present study adopted a within-person approach (a daily-basis research design) and collected data from 60 front-line employees over 10 working days. Although all variables were self-rated, common method variance is minor. Confirmatory factor analysis (CFA) was conducted to ensure discriminant and convergent validity, and hierarchical linear modeling (HLM) was used to test the hypotheses.

Findings
The results of CFA ensure the measures have discriminant and convergent validity, while the results of HLM analysis showed that work engagement fully mediates the negative relationship between abusive supervision and the two kinds of extra-role behaviors. The bootstrapping results also support the full mediation effect of work engagement.

Originality/value
The present study used the job demands-resources model to examine how abusive supervision influences employees’ OCB and voice and found that work engagement is one possible mechanism between these two types of extra-role behavior. Specifically, a daily research design discovered that in a given working day, once a leader exhibits abusive supervision behavior, compared with any given day without abusive behaviors, employees will find it difficult to focus on their current tasks (i.e. through exhibiting decreased work engagement), which will in turn influence their willingness to exhibit OCB and voice on that particular day. Thus, both researchers and managers should focus on the daily interactions between leaders and employees because it is impossible to achieve organization success in one day, but rather such success is the aggregate result of both leaders’ and employees’ daily efforts.
Innovative seismic resistant structure of shield building with base isolation and tuned-mass-damping for AP1000 nuclear power plants
Authors: Hou Gangling , Li Meng , Hai Sun , Song Tianshu , Wu Lingshu , Li Yong , Zheng Gang , Shen Feng , Chen Yaodong 
Published: June 2019

Purpose
Seismic isolation, as an effective risk mitigation strategy of building/bridge structures, is incorporated into AP1000 nuclear power plants (NPPs) to alleviate the seismic damage that may occur to traditional structures of NPPs during their service. This is to promote the passive safety concept in the structural design of AP1000 NPPs against earthquakes.

Design/methodology/approach
In conjunction with seismic isolation, tuned-mass-damping (TMD) is integrated into the seismic resistance system of AP1000 NPPs to satisfy the multi-functional purposes. The proposed base-isolation-tuned-mass-damper (BIS-TMD) is studied by comparing the seismic performance of NPPs with four different design configurations (i.e. without BIS, BIS, BIS-TMD and TMD) with the design parameters of the TMD subsystem optimized.

Findings
Such a new seismic protection system (BIS-TMD) is proved to be promising because the advantages of BIS and TMD can be fully used. The benefits of the new structure include effective energy dissipation (i.e. wide vibration absorption band and a stable damping effect), which results in the high performance of NPPs subject to earthquakes with various intensity levels and spectra features.

Originality/value
Parametric studies are performed to demonstrate the seismic robustness (e.g. consistent performance against the changing mass of the water in the gravity liquid tank and mechanical properties) which further ensures that seismic safety requirements of NPPs can be satisfied through the use of BIS-TMD.
An evaluation of the impact of risk cost on risk allocation in public private partnership projects
Authors: Almarri Khalid , Alzahrani Saleh , Boussabaine Halim 
Published: June 2019

Purpose
A unique aspect of PPP is the opportunity for the transfer of risk ownership to the private sector. The purpose of this paper is to investigate how risk cost influences risk allocation.

Design/methodology/approach
A questionnaire survey was used to collect data. The questionnaire included nine sub-categories of risks. To quantify the influence of risk cost on risk allocation, a dependency risk matrix was employed. Heat maps techniques were used to visualise the results of the survey.

Findings
The findings show which risks within the endogenous or exogenous groups are to be allocated to the public sector, the private sector, or to be shared. The finding from this research provides a baseline for the PPP stakeholders in developing guidelines for estimating the value of risk costs in the risks register as well as serving as a mechanism for risk allocation.

Research limitations/implications
The context of the study may limit the generalisability of the results.

Practical implications
The study provides practical guidance to PPP stakeholders on risk allocation appetite.

Originality/value
This study extends the processes and methods by which PPP project’s risk is allocated to create a better value for all the stakeholders.
The economics of the entrepreneur and the banker historical roots and contributions to the management of innovation
Authors: Boutillier Sophie 
Published: June 2019

PurposeThe purpose of this paper is to study the nature of the relationship between the entrepreneur and the banker, which is central to any analysis of business creation and innovation management. The author’s main purpose is to understand how this relationship has been studied by the pioneer economists of the entrepreneur and to highlight their contribution to the understanding of today’s reality.Design/methodology/approachTo do so, the author proposes a sketch of an entrepreneur and banker economics based on the study of six economists (Cantillon, Smith, Bentham, Say, Schumpeter and Baumol) known for their works on entrepreneur theory. In their works, they explained how the (often difficult) relationship between the entrepreneur and the banker is built in a context of multi-uncertainty. They define the entrepreneur in different ways (a risk-taker, a prudent man, a projector, etc.), and put forward different behaviors facing uncertainty through social relations. The relationship between the entrepreneur and the banker can be read according to the grid of analysis of strong or weak ties (Granovetter, 1973).FindingsThis analysis demonstrates the importance of trust between the two protagonists. This contribution remains fundamental to study the behavior of financers and entrepreneurs today in the context of business eco-systems, clusters, science parks ‒ in other words, the main places of emergence of innovation.Research limitations/implicationsThis research leads to the proposal of the main basis of an economics of the entrepreneur and the banker; it can be further developed with the addition of other contributions of historical economists.Practical implicationsThis research shows the importance of thinking about the ways to build trust within the relation between entrepreneurs and their funders (bankers, venture capital, crowdfunding).Social implicationsThe analysis of social ties (weak or strong) plays a major role in this relation.Originality/valueThe originality of the article is to come back to the works of pioneer economists and to show their contributions to the understanding of today’s reality.
Transforming a practice-based problem into research questions
Authors: Björk Marie 
Published: June 2019

Purpose
This paper describes and discusses aspects that affect research questions in a practice-based research study, where learning study is used as a framework. The purpose of this paper is to contribute to understanding of the process where teachers and a researcher collaborate in transforming practical teaching problems into research questions.

Design/methodology/approach
A case study is conducted. Data consist of field notes, logbooks, manuscripts and conference papers from two learning studies conducted in grade 4 by three teachers and one researcher, and notes from meetings in a subject-teacher group at the school. The analysis focuses on how the research questions emerge and change in relation to discussions among teachers and in the research group of teaching, previous research and learning theory.

Findings
Questions about students’ discernment of the structure in the base-ten system emerged in learning study 1 and in the subject-teacher group. Discussions of previous research and the didactical theory learning activity transformed the research questions in learning study 2, into focusing students’ theoretical knowledge, examining general structures in the base system, using learning models as tools. Conditions for identification of specific teaching problems and alternative theory in a learning study are discussed.

Originality/value
The explicit example where research questions are transformed can be used in further discussions and methodological questions regarding formulation of research questions in educational research. Discussions, specifically of transforming research questions, when learning study is used may be promoted.
Accounting for strategic management, strategising and power structures in the Jordanian higher education sector
Authors: Hutaibat Khaled 
Published: June 2019

Purpose
The purpose of this paper is to present the findings of a field study, investigating accounting, strategising and accounting for strategic management and power structures in the Jordanian higher education (HE) sector on the basis of Bourdieu’s theory of practice.

Design/methodology/approach
This paper adopts an interpretive stance, seeking to investigate the perceptions of actors in the field, with regard to accounting, strategising and accounting for strategic management in HE. The adopted methodology is adapted grounded theory, as this study assumes a prior theoretical stance of Bourdieu’s theoretical concepts. Data were collected through participant observation in meetings, at the workplace, interviews and documentation.

Findings
The main findings of this paper reflect how strategising and accounting in practice manifest themselves in the Jordanian HE sector. Bourdieu’s theory of practice sets the meta-theoretical context of the current study, with field setting the scene, and habitus being represented in the strategising mind-set participants adopt. The mind-set determines how strategic management accounting is perceived and dealt with. Strategic management accounting takes place at varying degrees. The power structures that influence and determine strategising and accounting in support thereof are researched on the basis of Bourdieu’s forms of capital. Different forms of capital matter in the HE sector determined by fields’ doxa.

Research limitations/implications
The researcher is a part of the field, the Jordanian HE sector; thus, their habitus has been exposed to its characteristics and features. Thus, certain internalised structures and experiences needed to be challenged for this analysis, which was not an easy task.

Originality/value
This study investigates accounting, strategic management and power structures in HE, and it highlights the different power structures, using Bourdieu’s forms of capital, which offers a great insight into how different cultures approach similar issues.
Merger control in the telecom industry: a landscape transformed
Authors: Tyagi Kalpana 
Published: June 2019

Purpose
This paper aims to underscore how the digitization of content and the convergence in the telecommunications sector has prompted a wave of consolidation between telecom and content players.

Design/methodology/approach
Using interdisciplinary insights from competition policy and business strategy, the paper draws attention to the interplay between business model innovation and merger control in the converged telecoms sector.

Findings
Technological innovation and business model innovation led to the emergence of over-the-top (OTT) services. This innovation in turn led to two key effects, first, successful commercialization of content and the emergence of the “smart pipes” that in turn has led to the second effect, which is increased mergers and acquisitions (M&As) in the converged telecommunications sector. Emergence of OTT with big data as a key advantage challenged the strategy and business models of the more established players, such as the AT&T, Time Warner, Liberty Global and Fox, which in turn led to the current trend of M&As in the sector.

Originality/value
This paper makes the following key contributions to the literature on M&As between the fixed/mobile and content players. First, it elucidates how the existing market players can benefit from competition policy, such as merger remedies to enter new and related markets. Second, it advocates that the US and the European competition authorities while assessing these M&As, take due account of innovation in business models, as business model innovation not only promotes innovation in the market but also enhances consumer welfare, considering that it offers the merged firm economies of scale and scope to offer better-quality goods and services at subsidized prices.