bluuebunny/crossref_metadata_2025_split huggingface dataset:
The complexity and challenges of fertility preservation in women with cervix cancer—A prospective cohort study reporting on reproductive outcome and overall survival
Authors: Marklund Anna , Jiang Yanyu , Röjlar Hanna , Sergouniotis Fotios , Nilsson Hanna , Lundberg Frida E. , Rodriguez‐Wallberg Kenny A. 
Published: November 2024

AbstractIntroductionOur objective was to assess the feasibility of fertility preservation (FP) in women referred for cervix cancer, the long‐term reproductive outcome, and overall survival.Material and MethodsProspective cohort study of patients referred for FP counseling between 1999 and 2021 to the FP program of Karolinska University Hospital, Stockholm, Sweden. Baseline data included age, parity, disease stage, treatment characteristics, and FP methods applied. Data on reproductive outcomes and overall survival (by October 19, 2023) were retrieved from clinical registries and a population‐based register. Trial registration number: ClinicalTrials.gov NTC04602962.ResultsIn total, 91 women were referred, 67% with stage I cancers and 25%, 7%, and 1% with stages II, III, and IV, respectively. Cancers were diagnosed during pregnancy or postpartum in six cases. Cancer treatments included fertility‐sparing surgery in 14%, hysterectomy in 30%, and radiochemotherapy in 79% of cases. The treatment modality did not rule out the possibility to undergo FP, and following counseling, 68 patients elected to undergo FP by cryopreservation of embryos or oocytes (N = 11), ovarian tissue (N = 54), or both (N = 3). After a mean follow‐up of 8.1 years, 25 women (37%) returned to the center, five women achieved conception either spontaneously or through assisted reproduction, and 11 women became mothers through adoption or surrogacy. In the group of women receiving radical surgery or chemo/radiotherapy, no live births using cryopreserved specimens have yet been achieved. During follow‐up, 7 women (10%) in the FP group and 5 women (24%) in the group without FP had died of their disease. Cancer recurrence was documented in 19 patients.ConclusionsOur findings underscore the complexity and challenges associated with FP in the context of cervix cancer. Results of this study demonstrate that many women diagnosed with cervix cancer at reproductive age desire to achieve parenthood. While fertility‐sparing surgery can allow pregnancy, those who undergo a hysterectomy are limited to adoption, surrogacy, or the emerging possibility of uterus transplantation.
Decreased expression of Syndecan‐ 1 (CD138) in the endometrium of adenomyosis patients suggests a potential pathogenetic role
Authors: Shaalan Walid , Ibrahim Mohamed Gamal , Plasger Ariana , Hassan Nourhan , Kiesel Ludwig , Schüring Andreas N. , Götte Martin 
Published: November 2024

AbstractIntroductionAdenomyosis is a special subtype of endometriosis, affecting the myometrium, affecting about 20% of women in the reproductive age period. Clinical symptoms and intensity are diverse and can vary from heavy menstrual bleeding and dysmenorrhea to infertility and repeated pregnancy losses. Thus, patients often present with a long history of illness pending presumptive clinical or surgical diagnosis. A definitive diagnosis of adenomyosis is made upon histopathological examination verifying ectopic endometrial tissue (endometrial glands and/or stroma) within the myometrium, surrounded by hyperplastic and hypertrophic smooth muscles. However, nowadays ultrasonographic and/or MRI signs can precisely detect it as well. The precise etiology and pathogenesis remain unclear. One theory assumes that adenomyosis occurs through metaplastic transformation or migration of stem cell‐like cells.Material and MethodsOur study examined the immunohistochemical expression of the transmembrane proteoglycan Syndecan‐1 (CD 138), a multifunctional matrix receptor and signaling co‐receptor, in the endometrium of 35 patients (n = 21 with adenomyosis and n = 14 as a control group) in the period 2016–2017.ResultsAs a pilot study, we concluded that Syndecan‐1 is downregulated in adenomyosis patients compared to the control group, supporting its potential role in the development of adenomyosis. Our study did not find a correlation between the immune‐expression of Syndecan‐1 and the menstrual cycle phase.ConclusionsFor clinical significance in relation to our results, the investigated data showed that the downregulation of Syndecan‐1 in adenomyotic patients in our study may suggest a role in promoting the invasiveness of endometriotic islands within the myometrium. However, further studies are still needed to understand the mechanistic contribution of Syndecan‐1 to the pathogenesis of adenomyosis.
Infectious morbidity and white blood cell count associated with grade repetition and school absenteeism
Authors: Villamor Eduardo , Beer Rachael J. , Seeley Allison L. , López‐Arana Sandra , Marín Constanza , Mora‐Plazas Mercedes 
Published: November 2024

AbstractAimInfections can impair cognitive development, but their role on adverse childhood educational outcomes is unknown. We examined the associations of infectious morbidity and inflammatory biomarkers with grade repetition and school absenteeism.MethodsWe followed 2762 Colombian children aged 5–12 years for a school year. We quantified inflammatory biomarkers at enrolment and prospectively recorded incidence of gastrointestinal and respiratory infections, doctor visits and absent days from school using pictorial diaries. We estimated adjusted relative risks (ARR) with 95% confidence intervals (CI) for grade repetition and absenteeism by infectious morbidity burden and inflammatory biomarker categories, and percentages of the associations mediated through absenteeism.ResultsMorbidity was associated with increased risk of grade repetition. ARR (95% CI) of grade repetition comparing high versus no incidence of gastrointestinal, respiratory and ear infections were, respectively, 2.17 (1.00, 4.72), 2.31 (1.28, 4.16) and 2.57 (1.13, 5.86). Infections also predicted school absenteeism, which mediated 35%, 31% and 38% of the corresponding morbidity‐grade repetition associations. Elevated white blood cells (WBC), especially granulocytes, were related to increased grade repetition and school absenteeism risks.ConclusionChildhood infections and elevated WBC are associated with grade repetition and school absenteeism. Absenteeism does not fully explain the morbidity‐grade repetition associations.
Watery archives: Reflections on doing participatory archival research for climate action and audience engagement
Authors: Worthen Hannah , Weatherall Claire 
Published: November 2024

AbstractThis paper is based on our experience of running a one‐day participatory workshop at the Hull History Centre in the summer of 2023 titled ‘Watery Archives: Exploring Hull's flood histories’. The event was co‐designed by academics and archivists in direct response to feedback from our community participants as part of the Risky Cities project at the University of Hull. During the day, we invited participants to explore original historical material about Hull's flood histories from the seventeenth to the twentieth century, and then to produce creative and sometimes activist outputs that encouraged participants to critically engage with climate change today. The paper reflects on the value of community and participatory based approaches to open up access to archives and to unleash new learnings and discoveries. It considers some of the potential barriers to engagement in archives and shows how we addressed some of those challenges in our workshop design using methods of participatory learning and research. The paper shows how the 'Watery Archives' workshop built on the power of partnership between academic and heritage organisations to drive community‐led, place‐based research.
Memorials and collective memory: A text analysis of online reviews
Authors: Mulderig Bethany , Carriere Kevin R. , Wagoner Brady 
Published: November 2024

AbstractMemorials extend beyond their physical constructs, embodying political narratives and influencing collective memory. This study examines how traditional memorials and counter‐memorials shape geopolitical storytelling and public sentiment. Through text analysis of over 158,000 online reviews, we compare emotional responses elicited by these memorial types. Our findings reveal distinct differences in reactions, with traditional memorials evoking pride and heroism, while counter‐memorials prompt reflection on loss and historical trauma. These results contribute to understanding how memorials influence political perceptions and collective memory, offering insights into the psychological mechanisms behind geopolitical engagement with historical events.
Disruption of survivin protein expression by treatment with YM155 accelerates the resolution of neutrophilic inflammation
Authors: Fernandes Débora de Oliveira , Machado Jessica Rayssa , Beltrami Vinicius Amorim , Santos Anna Clara Paiva Menezes Dos , Queiroz‐Junior Celso Martins , Vago Juliana Priscila , Soriani Frederico Marianetti , Amaral Flávio Almeida , Teixeira Mauro Martins , Felix Franciel Batista , Pinho Vanessa 
Published: November 2024

AbstractBackground and PurposeProlonged survival of neutrophils is essential for determining the progression and severity of inflammatory and immune‐mediated disorders, including gouty arthritis. Survivin, an anti‐apoptotic molecule, has been described as a regulator of cell survival. This study aims to examine the effects of YM155 treatment, a survivin selective suppressant, in maintaining neutrophil survival in vitro and in vivo experimental settings of neutrophilic inflammation.Experimental approachBALB/c mice were injected with monosodium urate (MSU) crystals and treated with YM155 (intra‐articularly) at the peak of inflammatory response. Leukocyte recruitment, apoptosis neutrophil and efferocytosis were determined by knee joint wash cell morphology counting and flow cytometry. Resolution interval (Ri) was quantified by neutrophil infiltration, monitoring the amplitude and duration of the inflammation. Cytokine production was measured by ELISA. Mechanical hypernociception was assessed using an electronic von Frey aesthesiometer. Efferocytosis was evaluated in zymosan‐induced neutrophilic peritonitis. Survivin and cleaved caspase‐3 expression was determined in human neutrophils by flow cytometry.Key ResultsSurvivin was expressed in neutrophils during MSU‐induced gout, and the treatment with YM155 reduced survivin expression and shortened Ri from ∼8 h observed in vehicle‐treated mice to ∼5.5 h, effect accompanied by increased neutrophil apoptosis and efferocytosis, both crucial for the inflammation resolution. Reduced IL‐1β and CXCL1 levels were also observed in periarticular tissue. YM155 reduced histopathological score and hypernociceptive response. In human neutrophils, lipopolysaccharide (LPS) increased survivin expression, whereas survivin inhibition with YM155 induced neutrophil apoptosis, with activation of caspase‐3.Conclusions and ImplicationsSurvivin may be a promising therapeutic target to control neutrophilic inflammation.
GraphCPP: The new state‐of‐the‐art method for cell‐penetrating peptide prediction via graph neural networks
Authors: Imre Attila , Balogh Balázs , Mándity István 
Published: November 2024

Background and PurposeCell‐penetrating peptides (CPPs) are short amino acid sequences that can penetrate cell membranes and deliver molecules into cells. Several models have been developed for their discovery, yet these models often face challenges in accurately predicting membrane penetration due to the complex nature of peptide–cell interactions. Hence, there is a need for innovative approaches that can enhance predictive performance.Experimental ApproachIn this study, we present the application GraphCPP, a novel graph neural network (GNN) for the prediction of membrane penetration capability of peptides.Key ResultsA new comprehensive dataset—dubbed CPP1708—was constructed resulting in the largest reliable database of CPPs to date. Comparative analyses with previous methods, such as MLCPP2, C2Pred, CellPPD and CellPPD‐Mod, demonstrated the superior predictive performance of our model. Upon testing against other published methods, GraphCPP performs exceptionally, achieving 0.5787 Matthews correlation coefficient and 0.8459 area under the curve (AUC) values on one dataset. This means a 92.8% and 23.3% improvement in Matthews correlation coefficient and AUC measures respectively compared with the next best model. The capability of the model to effectively learn peptide representations was demonstrated through t‐distributed stochastic neighbour embedding plots. Additionally, the uncertainty analysis revealed that GraphCPP maintains high confidence in predictions for peptides shorter than 40 amino acids. The source code is available at https://github.com/attilaimre99/GraphCPP.Conclusion and ImplicationsThese findings indicate the potential of GNN‐based models to improve CPP penetration prediction and it may contribute towards the development of more efficient drug delivery systems.
Young Carers' Identification: Comparing Adolescents' and Parents' Perspectives
Authors: Pilato Jade , Dorard Géraldine , Cincidda Clizia , Untas Aurélie 
Published: November 2024

ABSTRACTBackgroundYoung carers are children or adolescents who provide significant care to another family member who has an illness or disability. This situation impacts their physical and mental health, and their social and school life. Nonetheless, this vulnerable population is often covert, which makes YCs' identification a major challenge. One of the methods in the literature to identify them consists of obtaining the adolescent's report of a relative's illness or disability, coupled with the standardized psychometric evaluation of their level of care. To date, no research had compared adolescents' and parents' perspectives using this method, while this would enable to gain insights on YCs' identification. The objective of this study was to compare parents' and adolescents' reports on the identification of an ill or disabled relative within the household, and adolescents' levels of caring activities.MethodsSurvey data were collected from 776 adolescents (mean age = 12.76 years; 55.50% of females) and one of their parents (mean age = 44.31 years; 83.76% of females). They completed questionnaires evaluating their sociodemographic characteristics, illnesses or disabilities within their household, and adolescents' levels of caring activities (MACA–YC 18). Cohen's κ for interrater agreement, Student's t‐tests and chi‐square tests of independence were performed for child–parent responses comparisons.ResultsFewer adolescents reported that there was an ill or disabled relative within the household compared with their parents. Only 27.56% of the dyads agreed both on the person who was ill or disabled and the illness or disability. The dyads were more often in agreement when the ill or disabled relative had a serious somatic illness and was the mother. On the other hand, adolescents reported engaging in higher levels of caregiving activities than indicated by their parents' assessments.ConclusionThis study highlights the difference of perception between parents and adolescents both on illness and disability reports, and on adolescents' levels of caring activities. These two points of view seem complementary, suggesting that combining adolescents' and parents' perspectives can aid in better identifying young carers.
Examining the Likelihood of Being Referred to the Child Welfare Services in Sweden: Influences at the Individual, Peer and School‐Area Levels
Authors: Kalin Torbjörn 
Published: November 2024

ABSTRACTIn Sweden, both maltreatment and behavioural problems are indicative of needs that motivate interventions from child welfare services (CWS). However, the likelihood of being referred to CWS can be influenced by other than indicative factors on individual, peer and school‐area levels. Studies disentangling such other factors (possible bias) from indicative factors are still lacking in Sweden. Using a subsample of 1286 children across 14 schools from a longitudinal research programme and linking survey data to child welfare records, this article examines the relationship between maltreatment, behavioural problems, individual socio‐economic factors, peer affiliation, disadvantaged school areas and the likelihood of being referred. After controlling for maltreatment and behavioural problems, findings demonstrate that other factors on all three levels (individual, peer and school‐area) increase the likelihood of being referred, for example, living with low socio‐economic status, living in a single‐parent household, being affiliated with peers who smoke and attending school with a large proportion of children from socioeconomically disadvantaged areas. The findings highlight that individual‐level socio‐economic factors are pivotal in understanding why some children are referred, while others with similar needs are not. Implications for outreach social work with children at risk of maltreatment and behavioural problems are discussed.
Global Trends and Perspectives on Dental Fragment Reattachment (1988–2024): A Bibliometric and Altmetric Review
Authors: Mulinari Julia , de Souza Isadora Durante , Pinho Nicolle Silveira , dos Anjos Lucas Menezes , Santana Carla Miranda , Cardoso Mariane , de Oliveira Rocha Aurélio 
Published: November 2024

ABSTRACTFragment reattachment in traumatic injuries is notable for its excellent esthetic, morphological, and functional results. The aim of this study was to investigate the scientific history and trends in publications on dental fragment reattachment through a bibliometric and altmetric analysis. The search was conducted in October 2024 using Web of Science. Two researchers selected articles and extracted key study characteristics. Collaborative networks were generated using VOSviewer software. Dimensions was consulted to measure altmetric data. The Spearman correlation was used. A total of 129 articles were included. The most cited article received 160 citations. Studies were published between 1988 and 2024. The leading journal was Dental Traumatology (n = 55). Case reports (n = 63) were the most frequent study design. The majority of studies aimed at clinical case follow‐up (n = 71), with coronal fractures being most commonly reported (n = 96). Young patients were most affected in the selected studies (n = 49). Tewari N stood out (n = 8). The All India Institute of Medical Sciences was prominent with seven articles. Brazil was the most prevalent country (n = 29), and Asia was the most frequent continent (n = 57). Vosviewer demonstrated collaborations among authors. According to Dimensions, significant mentions were identified on Mendeley, followed by X users. This bibliometric analysis provides a comprehensive description of the technique of fragments reattachment in traumatized teeth, which has been studied for over 36 years. Studies on this technique primarily rely on case reports describing follow‐up in young patients. Intervention studies and systematic reviews are recommended. Additionally, more studies in Africa, Oceania, and North America are encouraged.
Threshold of defensive response in Apis mellifera (honey bees) and subsequent brain gene expression in reaction to noxious stimuli
Authors: Acevedo‐Gonzalez Jenny P. , Galindo‐Cardona Alberto , Fuenzalida‐Uribe Nicolas L. , Ortiz‐Alvarado Yarira , Ghezzi Alfredo , Giray Tugrul 
Published: November 2024

AbstractHoney bee, Apis mellifera L. (Hymenoptera: Apidae), colonies and individuals respond variably to disturbances. The response depends on perception and interpretation of stimuli requiring both neural modulation and use of energy. In this study, we examined the role of neural modulation and brain metabolism in constitutive and experience‐dependent differences in defensive response. For constitutive differences, we compared brain gene expression in bees from gentle and defensive colonies identified in a standard colony‐level assay. For experience‐dependent changes in defensiveness response, we compared brain gene expression in control bees and bees that responded by sting extension to electric shock in a standardized individual behavioral assay. In both experiments, for neuromodulation, we examined membrane receptor genes for the biogenic amines dopamine (DopR2), octopamine (OA1), and serotonin (5HT2a), as well the gene for the enzyme responsible for serotonin synthesis (THR). For neural metabolism, we examined the expression of two Oxidative Phosphorylation Pathway “OXPHOS” genes (ND51 and ND20‐LIKE). Bees collected from defensive colonies had a significantly lower expression of amine receptor, synthesis gene, and OXPHOS genes. However, bees responding to noxious stimuli (i.e., electric shock) showed greater gene expression for both OXPHOS and neuromodulation genes, except for 5HT2a. We discuss the intriguing intersection of neuromodulation and neural metabolism in defensive response both for constitutive differences, and contrasting experience dependent or adaptive differences.
Comparison of serum microRNA in healthy horses and horses with moderate to severe mitral valve regurgitation using a commercially available canine cardiac panel
Authors: Calewaert Amber , Palarea‐Albaladejo Javier , Coultous Robert , Capewell Paul , Hanks Eve , Decloedt Annelies , van Loon Gunther 
Published: November 2024

AbstractBackgroundMicroRNA (miRNA) has previously been used as a biomarker for cardiac disease in humans and dogs, however, studies in horses are not yet available.ObjectivesTo determine if adult horses with moderate or severe mitral valve regurgitation have a different serum miRNA expression profile compared to healthy controls.Study designRetrospective cross‐sectional.MethodsSerum samples from 77 adult horses with moderate or severe mitral valve regurgitation and 77 healthy control horses were analysed using a commercial cardiac disease‐specific miRNA panel previously used in dogs.ResultsThe commercial canine cardiac miRNA panel had low discriminatory power as a biomarker for mitral valve regurgitation in adult horses. Sensitivity was 0.58 (95% Cl: 0.47–0.69) and specificity 0.57 (95% Cl: 0.46–0.68).Main limitationsClinical data were extracted retrospectively and currently there is no well‐established criteria for grading mitral regurgitation in horses; there were few severe mitral regurgitation cases and the pathogenesis of mitral regurgitation was not considered. Controls were not matched by age, breed or sex. An assay developed for use in dogs was used.ConclusionDespite strong miRNA conservation across species, the commercially available canine cardiac miRNA panel failed as biomarker for mitral valve regurgitation in adult horses. Further research is needed to determine if an equine specific panel can be developed that performs better as biomarker for cardiac disease in horses.