bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell line development and bioreactor process optimization for an atezolizumab biosimilar
Authors: Kuyucu Ayca Zeybek , Sayili Dogu , Orkut Ridvan , Mert Olcay , Tarman İbrahim Oguzhan , Lulaci Busra , Sencer Ali Mert , Pekmezci Asli Kurden , Avci Mehmet Ender , Kalyoncu Sibel , Inan Mehmet 
Published: December 2024

AbstractCheckpoint inhibitors are widely recognized immunotherapeutic drugs, known for their effectiveness in treating various cancers. Atezolizumab, targeting the immune checkpoint programmed death‐ligand 1, is successfully used to treat several types of cancers. Atezolizumab is a potential biosimilar candidate due to its huge success in the clinic but there is no literature on its production process in mammalian cells. In this study, we generated a monoclonal cell line derived from recombinant Chinese hamster ovary DG44 cells to produce atezolizumab. The selected single clone was employed for media screening and process development. Following production in a 7‐L bioreactor, atezolizumab was purified using a three‐step chromatographic method. Finally, the purified atezolizumab was characterized and compared with commercial atezolizumab (Tecentriq) through several chromatographic and kinetics analyses.
Impact of Shock Compression on the Photovoltaic Performance of NiCo2O4 Nanoparticles
Authors: Franklin J. Bosco , Saravanan P. , Suruthi S. , Bincy F. Irine Maria , Sundaram S. John , Dhas S. A. Martin Britto , Wadaan Mohammad Ahmad , Kumar Jothi Vinoth , Mythili R. 
Published: December 2024

ABSTRACTSpherical‐shaped nickel cobaltite (NC) nanoparticles were synthesized via a simple sol–gel technique and calcined at 600°C. X‐ray diffraction (XRD) analysis revealed significant changes in crystallite size, with an average of 23 nm for the control sample and variations observed after 50 shockwaves. Fourier transform infrared spectroscopy (FTIR) confirmed metal‐oxygen stretching, indicating structural integrity. UV–visible absorption studies showed changes in the optical band gap, which increased after shock treatments, suggesting bandgap tunability for optoelectronic and photovoltaic applications. The material exhibited good optical absorption up to 600 nm, making it suitable for light‐harvesting devices. Vibrating sample magnetometry (VSM) detected shifts in dipole moments and magnetic saturation, with all samples displaying paramagnetic behavior. The shock‐treated samples showed enhanced magnetic properties, which could be useful in magnetic storage devices. The combined tunability of bandgap and magnetic properties via shock wave treatment underscores the potential of these nanoparticles for applications in photovoltaics, spintronics, and energy storage systems.
An Exploration of Risk Factors for Further Police Contact Among Females Known for Online Child Sexual Exploitation Offenses
Authors: Fortunato Erika , Slikboer Reneta , Henshaw Marie , Meyer Denny , Ogloff James R. P. 
Published: December 2024

ABSTRACTDespite increases in online child sexual exploitation (OCSE) internationally, no study has examined risk factors for re‐offending among females who perpetrate OCSE, resulting in limited knowledge regarding the idiosyncratic needs of this cohort. This study explored factors predictive of further police contact among 116 females known to police for OCSE offenses in Victoria, Australia. Four binary regressions were run for each of Any, Violent, and Non‐Violent re‐offending, with one regression each focusing on characteristics related to offending history, index offending, co‐offending, and victimization. Predicted probabilities from each model were then input as predictors into a final binary regression for each recidivism type. Offending history emerged as the most robust predictor across re‐offending types, even when controlling for other domains. Victimization and index offending also predicted Non‐Violent re‐offending, while co‐offending was not predictive across any analysis. These preliminary findings have implications for the unique management needs of females who perpetrate OCSE.
The anatomical basis for surgical intervention in myositis ossificans—A systematic review
Authors: Stammer Adam , Ashwood Neil , Amara Veda , Suryawanshi Suraj , Wilson Paul , Dekker Andrew 
Published: December 2024

AbstractMyositis ossificans (MO) is a benign condition characterized by heterotrophic bone formation, most commonly within muscle tissue. Multiple types have been described, the most predominant being myositis ossificans circumscripta, which occurs in response to trauma. Myositis ossificans cases reported in the literature were reviewed systematically. The aim of the review was to examine how the condition and its management are influenced by the anatomical structures involved. The Medline and Ovid Embase online databases were searched for all papers relevant to MO between Jan 1972 and Dec 2020. Duplicate and unobtainable records were removed. The remaining records were manually assessed for relevance by three independent reviewers, initially by abstract and then by full‐text screening, to ensure that all papers were suitable. Acceptance of articles was not limited by case features or study design. In total, 77 papers from the literature search were included. They contained information on 89 patients diagnosed with MO. The average age of the patients was 26.17 years (range 13 weeks to 72 years, a 71.75 year range) and 65.17% were male. The lower limb was affected more than the upper limb or spine, the most common site being the thigh. Muscle was the most commonly affected tissue, the quadricep femoris being most often involved. Diagnostic imaging varied widely among cases; X‐ray alone was the most common method, followed by a combination of X‐ray and MRI. Lesions in the lower limb had larger diameters than those in the upper limb or spine. More cases in the upper limb (47.83%) than in the lower limb (33.33%) required surgery. All instances of MO in the jaw, hand, and foot were symptomatic, and all required surgical management. The findings were consistent with previous research showing MO to be a predominantly male disease, with most patients being under 30 years of age. Trauma was involved in 52.81%, lower than previously reported. In this study, the vastus lateralis of the quadriceps was most often affected. MO lesions were less common and smaller in the upper limb but more required surgery. Anatomically confined sites such as the hand and foot always required surgery, probably because of earlier onset or more severe functional effects of symptoms. Further work is needed to review the anatomical predilection of MO to help identify patients who could benefit from earlier consideration of surgery. A national registry of MO cases could contribute to further research on this disease.
Transcatheter Tricuspid Valve Replacement Following Tricuspid Edge‐To‐Edge‐Repair
Authors: Goldschmied Andreas , Droppa Michal , Gawaz Meinrad , Geisler Tobias 
Published: December 2024

ABSTRACTSignificant tricuspid regurgitation (TR) is seen as a relevant contributor of cardiac morbidity and mortality. Transcatheter tricuspid valve replacement (TTVR) is a novel technique to treat this condition. We present the case of an 82‐year‐old lady who was admitted for recurrent right heart decompensation despite having undergone treatment with tricuspid edge‐to‐edge repair (TEER). The patient underwent transfemoral implantation of a 48 mm EVOQUE valve. Since the implanted Clip was not in the central part of the valve, it was pushed toward the valvular anulus by the expanded prothesis. Echocardiography showed a good result with no residual TR. Options for residual TR after T‐TEER are very limited. TTVR might be suitable in selected patients with non‐central Clip placement.
From lectures to co‐constructively developed inservice teacher training with the DiKoLAN framework
Authors: Henne Anna , Syskowski Sabrina , Möhrke Philipp , Thoms Lars‐Jochen , Becker‐Genschow Sebastian , Huwer Johannes 
Published: December 2024

AbstractIm Rahmen der Initiative lernen:digital entstehen deutschlandweit neue Fortbildungsangebote für Lehrkräfte. In diesem Artikel wird ein Ansatz zur ko‐konstruktiven Entwicklung von Fortbildungen basierend auf Konzepten der partizipativen Aktionsforschung vorgestellt. Zur Entwicklung des Aufbaus und der Inhalte der Fortbildung werden Erkenntnisse aus der fachdidaktischen Literatur, Lernziele aus den Bildungsplänen und die Expertise von Forschenden, Lehrpersonen und Fachleiterinnen und Fachleitern an staatlichen Seminaren für Aus‐ und Weiterbildung zusammengeführt, um eine Fortbildung zu entwickeln, die die Bedürfnisse aller Beteiligten berücksichtigt.
Ten years of experience with ruxolitinib since approval for polycythemia vera: A review of clinical efficacy and safety
Authors: Masarova Lucia , Mascarenhas John , Rampal Raajit , Hu Wilson , Livingston Robert A. , Pemmaraju Naveen 
Published: December 2024

AbstractThe oral Janus kinase (JAK) 1/JAK2 inhibitor ruxolitinib was approved by the US Food and Drug Administration in 2014 for treatment of patients with polycythemia vera (PV) who have an inadequate response to or intolerance of hydroxyurea (HU). PV is a chronic myeloproliferative neoplasm defined by primary absolute erythrocytosis, bone marrow hypercellularity, and JAK mutations such as JAK2V617F. Patients with PV experience burdensome symptoms and are at risk of thromboembolic events, in particular those with resistance to or intolerance of initial treatments such as HU. Other risks for patients with PV include progression of disease to more aggressive forms with worse prognoses, such as myelofibrosis or blast‐phase myeloproliferative neoplasms. This review summarizes the efficacy and safety of ruxolitinib from key phase 2 and 3 trials (MAJIC‐PV, RESPONSE, RESPONSE‐2, RELIEF, and Ruxo‐BEAT), large real‐world studies, and a decade of postmarketing surveillance safety data. The authors focus on improved blood count control, rates of thromboembolic events, symptom improvement, and markers of disease modification such as reduction of JAK2V617F allele burden in patients treated with ruxolitinib. They also discuss the well‐characterized safety profile of ruxolitinib regarding hematologic and other adverse events of interest. In the 10 years since its approval, ruxolitinib remains a safe and effective standard‐of‐care treatment for PV. As the treatment landscape for PV continues to evolve in the coming years, the efficacy and safety profiles of ruxolitinib suggest it will remain a preferred treatment as monotherapy and as a potential backbone of future combination regimens.
Comparison of Salivary Opiorphin in Burning Mouth Syndrome and Healthy Subjects and Its Correlation With Psychiatric Disorders
Authors: Dalirsani Zohreh , Amirchaghmaghi Maryam , Semnani Mahshid Malakuti , Talebi Mahdi , Hashemy Seyed Isaac , Shakeri Mohammad Taghi , Taghizadeh Ava 
Published: December 2024

ABSTRACTObjectivesBurning mouth syndrome (BMS) is a chronic disease in which the patient experiences a burning sensation in the oral mucosa without any pathological cause. Opiorphin is a pentapeptide that prevents pain and can have an antidepressant effect. The aim of this study was to evaluate salivary opiorphin concentration in BMS and healthy subjects. In addition, opiorphin concentration in BMS patients before and after psychiatric treatment was compared and the association between burning severity and psychiatric scores with salivary opiorphin concentration was examined.Materials and MethodsThis cross‐sectional study included 28 BMS patients and 40 healthy individuals referred to the Department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Mashhad, from September 2018 to August 2019. Patients with proven disease based on clinical and psychiatric examinations were included in the study. Unstimulated salivary opiorphin levels were assessed by ELISA. Statistical analysis was performed using SPSS version 24.ResultsSalivary opiorphin levels were significantly higher in BMS patients (2.16 ± 0.30 ng/mL) than in healthy subjects (1.80 ± 0.36 ng/mL) (p < 0.001). Opiorphin levels in BMS patients increased insignificantly after psychiatric therapy (p = 0.212). In addition, there was no significant association between opiorphin levels and age, gender, menopause, burning severity, anxiety, and depression status.ConclusionsThe opiorphin concentration in saliva is higher in BMS patients than in healthy subjects. Most BMS patients also suffer from anxiety and depression.
The effect of trimetazidine on cardiac haemodynamics and mitochondrial function in wild‐type transthyretin amyloidosis
Authors: Ladefoged Bertil Thyrsted , Pedersen Anders Lehmann Dahl , Dybro Anne Mette , Clemmensen Tor Skibsted , Gillmore Julian , Poulsen Steen Hvitfeldt 
Published: December 2024

AbstractAimsWild‐type transthyretin cardiac amyloidosis (ATTRwt) is a cardiomyopathy causing myocardial hypoperfusion and impaired cardiac mitochondrial function. Trimetazidine is an antianginal agent used in patients with stable angina pectoris, which improves cardiac contractility and mitochondrial function. The aim of the study was to investigate the effect of trimetazidine on invasive haemodynamics and cardiac mitochondrial function in ATTRwt.MethodsIn a randomized, double‐blind, placebo‐controlled, crossover trial, 22 patients with ATTRwt received 4 weeks of trimetazidine and placebo in randomized order. After each treatment period followed examinations with endomyocardial biopsies taken for high‐resolution respirometry and right heart catheterization at rest and during a cardiopulmonary exercise test. The primary endpoint was mean pulmonary artery wedge pressure (mPAWP) during peak exercise. The secondary endpoint was cardiac mitochondrial oxidative phosphorylation capacity. Exploratory endpoints were echocardiographic parameters, cardiac biomarker levels and quality of life.ResultsTrimetazidine did not significantly reduce mPAWP during peak exercise (31 ± 12 vs. 31 ± 13 mmHg, P = 0.61) or improve the cardiac mitochondrial oxidative phosphorylation capacity (73.4 ± 7.7 vs. 75.3 ± 7.7 pmol O2/(mg*s), P = 0.81) compared with placebo, nor did treatment with trimetazidine improve ejection fraction (P = 0.93), global longitudinal strain (P = 0.23), N‐terminal pro‐brain natriuretic peptide (NT‐proBNP) levels (P = 0.92) or the patients' quality of life (P = 0.98).ConclusionsIn ATTRwt, treatment with trimetazidine did not improve mPAWP or cardiac mitochondrial oxidative phosphorylation capacity compared with placebo.
Identification of Fracture Extension Modes During Hydraulic Fracturing in Coalbed Methane Vertical Wells: A Case Study From the Southern Shizhuang Area of the Qinshui Basin, China
Authors: Yan Jin , Ni Xiaoming , Zhang Yafei , Wang Wensheng , Niu Ruize 
Published: December 2024

ABSTRACTThe accurate identification of fracture extension patterns in hydraulic fracturing can provide important guidance for the optimisation of fracturing parameters. In this paper, factors such as effective hole friction and wellbore flow friction during fracturing are fully considered, and a calculation model of net bottom‐hole pressure of fracturing is constructed. By introducing the change rate of net bottom‐hole pressure and the changing characteristics of the fracturing curve, seven fracture extension modes during hydraulic fracturing in coalbed methane vertical wells are established. The accuracy of the identification method is verified by the fracture monitoring and production results in Shizhuang South Block. The results show that fracture elongation is mainly controlled by in situ stress difference, angle between natural fracture and maximum principal stress, coal tensile strength, fracturing time, proppant and angle between other factors. When the fracture construction parameters are fixed, the smaller the difference between maximum and minimum horizontal principal stresses and the smaller the natural fractures and maximum horizontal principal stresses. When the reservoir potential is similar, the effective extension index is positively correlated with the gas production effect, and the effective extension index can effectively judge the fracturing effect. The higher the proportion of effective extension mode, the longer the extension time and the higher the stable daily gas production. The research results provide a method and reference for clearly identifying the fracture extension and the occurrence conditions of different extension modes in the hydraulic fracturing process.
Mediating Role of Financialization and Intellectual Capital in the Relationship Between Ownership Structure and Firms’ Financial Performance
Authors: Amoughin Ramin Zamani , Rostamy Ali Asghar Anvary , Araghi Maryam Khalili , Saeidi Ali 
Published: December 2024

ABSTRACTThis paper investigates how intellectual capital (IC) and financialization mediate the relationship between ownership structure (OS) and the financial performance (FP) of manufacturing companies listed on the Iranian stock exchange from 2016 to 2022. OS is analyzed through three variables: institutional ownership (IO), ownership concentration (OC), and managerial ownership (MO). IC and financialization are considered as mediating variables, with firms’ FP as the dependent variable. This research was conducted due to a sample of 112 listed companies and employs artial least squares structural equation modeling (PLS‐SEM) for data evaluation. The results reveal that both IO and OC positively impact FP, whereas MO negatively affects FP. Furthermore, IC and financialization mediate the relationships between OC and MO with FP. However, these mediating factors do not affect the relationship between IO and FP.
Volatilome Study of Jabuticaba (Plinia cauliflora) Using a Hydrophilic Microporous Cartridge with Direct Immersion Solid Phase Microextraction and Gas Chromatography System With a Hybrid Quadrupole Time‐Of‐Flight Mass Spectrometry
Authors: de Oliveira Jhonatan Bispo , Rodrigues Samantha Christina , Menezes Helvécio Costa , de Souza Patterson Patrício , Cardeal Zenilda de Lourdes 
Published: December 2024

ABSTRACTJabuticaba (Plinia cauliflora) is a typical subtropical Brazilian fruit with unique organoleptic properties and a high nutritional value. This study shows a qualitative analysis of jabuticaba peels with volatile and semi‐volatile components harvested from Minas Gerais, Brazil. A new device, the hydrophilic microporous cartridge, was developed to extract jabuticaba peels' volatile/semi‐volatile components using a solid‐phase microextraction method by direct immersion. This cartridge protected the polymer phase fiber, preventing its breakage and impregnation of the material. The developed method is simple, using a few steps to prepare jabuticaba peel samples. The fiber selected for analysis was a divinylbenzene/carboxene/polydimethylsiloxane of 30/50 µm. A comprehensive two‐dimensional gas chromatography time‐of‐flight mass spectrometry system was used to analyze natural products in jabuticaba peels. The method was optimized by a factorial design and could detect 213 organic compounds. Of particular note is the detection of terpenes (33.27%), fatty acids (29.60%), and ethyl esters (9.23%), which are mainly responsible for the nutritional properties and odor of the fruit. This study presents an improved method for extracting volatile compounds, offering enhanced insights into the phytochemical composition, aroma, flavor, and bioactivity of jabuticaba.
Capillary Electrophoresis‐Frontal Analysis (CE‐FA) and Molecular Docking Studies on the Albumin‐Binding Properties of Dopamine and Serotonin
Authors: Kalaycıoğlu Zeynep , Bilen Defne 
Published: December 2024

ABSTRACTDopamine and serotonin are neurotransmitters that are crucial for numerous physiological processes, including mood regulation, reward, and motor function. Dysregulation of these neurotransmitters is associated with various neuropsychiatric disorders. Albumin in plasma modulates the bioavailability of drugs and free concentrations of bioactive constituents. This study aimed to characterize the interactions of dopamine and serotonin with bovine serum albumin. Capillary electrophoresis in the frontal analysis mode was utilized as an effective method to assess dopamine–bovine serum albumin and serotonin–bovine serum albumin affinity. The free neurotransmitter plateaus were distinctly separated from the bovine serum albumin–neurotransmitter complex plateaus. Free dopamine and serotonin concentrations were determined by monitoring the heights of their respective plateaus. In contrast, the bound concentrations were calculated from the difference between the total and free plateau heights. Dopamine and serotonin were found to bind to bovine serum albumin at independent sites with binding constant values of 1.90 × 103 and 2.90 × 103 L/mol, respectively. Additionally, an in silico molecular docking approach revealed the binding sites for dopamine and serotonin near the glutamic acid‐291 and serine‐428 residues of bovine serum albumin, respectively.
Pivotal Role of Mass Spectrometry for the Assessment of Exposure to Reactive Chemical Contaminants: From the Exposome to the Adductome
Authors: Debrauwer Laurent , Mervant Loic , Laprevote Olivier , Jamin Emilien L. 
Published: December 2024

ABSTRACTA large part of the Human chemical exposome is now well characterized, and its health effects has been widely documented, although precise causal links remain difficult to establish. In parallel, genetic factors only were shown to contribute less than 30% to various pathologies. Therefore, environmental factors may represent the predominant cause of chronic diseases. Mass Spectrometry has been established for many years as a main “gold standard” in this field due to its performances both in sensitivity and selectivity. However, some unstable or highly reactive compounds may escape their detection in the biological samples because of their short half‐life although some of their stable metabolites, if any, can be used for the exposure assessment. These electrophilic molecules are known to bind covalently to nucleophilic molecules in the body to form what are commonly called adducts. The study of adducts formed with DNA, proteins or with glutathione, nowadays called adductomics, can provide additional toxicologically relevant information in biomonitoring studies. This review describes this particular part of the reactive exposome and the related mass spectrometric methods developed therein. Three dedicated parts of this review are devoted to the contribution of mass spectrometry respectively to the assessment of DNA modifications, protein modifications, and reaction with glutathione.