bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vanadium Dioxide Nanocoating Induces Tumor Cell Death through Mitochondrial Electron Transport Chain Interruption
Authors: Li Jinhua , Jiang Meng , Zhou Huaijuan , Jin Ping , Cheung Kenneth M. C. , Chu Paul K. , Yeung Kelvin W. K. 
Published: December 2018

AbstractA biomaterials surface enabling the induction of tumor cell death is particularly desirable for implantable biomedical devices that directly contact tumor tissues. However, this specific antitumor feature is rarely found. Consequently, an antitumor‐cell nanocoating comprised of vanadium dioxide (VO2) prepared by customized reactive magnetron sputtering has been proposed, and its antitumor‐growth capability has been demonstrated using human cholangiocarcinoma cells. The results reveal that the VO2 nanocoating is able to interrupt the mitochondrial electron transport chain and then elevate the intracellular reactive oxygen species levels, leading to the collapse of the mitochondrial membrane potential and the destruction of cell redox homeostasis. Indeed, this chain reaction can effectively trigger oxidative damage in the cholangiocarcinoma cells. Additionally, this study has provided new insights into designing a tumor‐cell‐inhibited biomaterial surface, which is modulated by the mechanism of mitochondria‐targeting tumor cell death.
Evaluation of fluxgate magnetometry and electromagnetic induction surveys for subsurface characterization of archaeological features in World War 1 battlefields
Authors: Note Nicolas , Saey Timothy , Gheyle Wouter , Stichelbaut Birger , Van den Berghe Hanne , Bourgeois Jean , Van Eetvelde Veerle , Van Meirvenne Marc 
Published: December 2018

AbstractGeophysical prospection as a noninvasive archaeological survey technique has become a widely applied discipline that is currently finding its way into the former World War 1 (WW1) battlefields. Because of the imminent danger of unexploded ordnances, noninvasiveness is the key to exploring the subsurface containing our buried heritage. Fluxgate magnetometry (FGM), a frequently applied prospection technique in archaeology and unexploded ordnance detection, is not always satisfactory in this area. This is due to the high density of ferrous objects masking the underlying features of interest. Frequency domain electromagnetic induction (EMI) has proved to be less sensitive to metal objects, thereby revealing more WW1 traces. Another advantage of EMI is that soil disturbances related to shelling and metal objects can be classified with higher precision by combining magnetic and electrical conductivity data layers. After evaluating both prospecting techniques on maximum feature coverage, feature type identification, metal object filtering, and localization, EMI was shown to be the most successful. Although FGM is not inferior (providing high survey speed and density with multisensor arrays and reliability in pinpointing features, etc.) overall, EMI is the preferred option on sites with heavily disturbed soil. In this paper, these findings will be demonstrated with survey examples, case studies, and numerical analyses from the WWI battlefields of Flanders.
Oxytocin receptor agonist reduces perinatal brain damage by targeting microglia
Authors: Mairesse Jérôme , Zinni Manuela , Pansiot Julien , Hassan‐Abdi Rahma , Demene Charlie , Colella Marina , Charriaut‐Marlangue Christiane , Rideau Batista Novais Aline , Tanter Mickael , Maccari Stefania , Gressens Pierre , Vaiman Daniel , Soussi‐Yanicostas Nadia , Baud Olivier 
Published: December 2018

AbstractPrematurity and fetal growth restriction (FGR) are frequent conditions associated with adverse neurocognitive outcomes. We have previously identified early deregulation of genes controlling neuroinflammation as a putative mechanism linking FGR and abnormal trajectory of the developing brain. While the oxytocin system was also found to be impaired following adverse perinatal events, its role in the modulation of neuroinflammation in the developing brain is still unknown. We used a double‐hit rat model of perinatal brain injury induced by gestational low protein diet (LPD) and potentiated by postnatal injections of subliminal doses of interleukin‐1β (IL1β) and a zebrafish model of neuroinflammation. Effects of the treatment with carbetocin, a selective, long lasting, and brain diffusible oxytocin receptor agonist, have been assessed using a combination of histological, molecular, and functional tools in vivo and in vitro. In the double‐hit model, white matter inflammation, deficient myelination, and behavioral deficits have been observed and the oxytocin system was impaired. Early postnatal supplementation with carbetocin alleviated microglial activation at both transcriptional and cellular levels and provided long‐term neuroprotection. The central anti‐inflammatory effects of carbetocin have been shown in vivo in rat pups and in a zebrafish model of early‐life neuroinflammation and reproduced in vitro on stimulated sorted primary microglial cell cultures from rats subjected to LPD. Carbetocin treatment was associated with beneficial effects on myelination, long‐term intrinsic brain connectivity and behavior. Targeting oxytocin signaling in the developing brain may be an effective approach to prevent neuroinflammation – induced brain damage of perinatal origin.
Oncogenic dependence of glioma cells on kish/TMEM167A regulation of vesicular trafficking
Authors: Portela Marta , Segura‐Collar Berta , Argudo Irene , Sáiz Almudena , Gargini Ricardo , Sánchez‐Gómez Pilar , Casas‐Tintó Sergio 
Published: December 2018

AbstractGenetic lesions in glioblastoma (GB) include constitutive activation of PI3K and EGFR pathways to drive cellular proliferation and tumor malignancy. An RNAi genetic screen, performed in Drosophila melanogaster to discover new modulators of GB development, identified a member of the secretory pathway: kish/TMEM167A. Downregulation of kish/TMEM167A impaired fly and human glioma formation and growth, with no effect on normal glia. Glioma cells increased the number of recycling endosomes, and reduced the number of lysosomes. In addition, EGFR vesicular localization was primed toward recycling in glioma cells. kish/TMEM167A downregulation in gliomas restored endosomal system to a physiological state and altered lysosomal function, fueling EGFR toward degradation by the proteasome. These endosomal effects mirrored the endo/lysosomal response of glioma cells to Brefeldin A (BFA), but not the Golgi disruption and the ER collapse, which are associated with the undesirable toxicity of BFA in other cancers. Our results suggest that glioma growth depends on modifications of the vesicle transport system, reliant on kish/TMEM167A. Noncanonical genes in GB could be a key for future therapeutic strategies targeting EGFR‐dependent gliomas.
Augmented astrocyte microdomain Ca2+ dynamics and parenchymal arteriole tone in angiotensin II‐infused hypertensive mice
Authors: Diaz Juan Ramiro , Kim Ki Jung , Brands Michael W. , Filosa Jessica A. 
Published: December 2018

AbstractHypertension is an important contributor to cognitive decline but the underlying mechanisms are unknown. Although much focus has been placed on the effect of hypertension on vascular function, less is understood of its effects on nonvascular cells. Because astrocytes and parenchymal arterioles (PA) form a functional unit (neurovascular unit), we tested the hypothesis that hypertension‐induced changes in PA tone concomitantly increases astrocyte Ca2+. We used cortical brain slices from 8‐week‐old mice to measure myogenic responses from pressurized and perfused PA. Chronic hypertension was induced in mice by 28‐day angiotensin II (Ang II) infusion; PA resting tone and myogenic responses increased significantly. In addition, chronic hypertension significantly increased spontaneous Ca2+ events within astrocyte microdomains (MD). Similarly, a significant increase in astrocyte Ca2+ was observed during PA myogenic responses supporting enhanced vessel‐to‐astrocyte signaling. The transient potential receptor vanilloid 4 (TRPV4) channel, expressed in astrocyte processes in contact with blood vessels, namely endfeet, respond to hemodynamic stimuli such as increased pressure/flow. Supporting a role for TRPV4 channels in aberrant astrocyte Ca2+ dynamics in hypertension, cortical astrocytes from hypertensive mice showed augmented TRPV4 channel expression, currents and Ca2+ responses to the selective channel agonist GSK1016790A. In addition, pharmacological TRPV4 channel blockade or genetic deletion abrogated enhanced hypertension‐induced increases in PA tone. Together, these data suggest chronic hypertension increases PA tone and Ca2+ events within astrocytes MD. We conclude that aberrant Ca2+ events in astrocyte constitute an early event toward the progression of cognitive decline.
Salvage laryngectomy and laryngopharyngectomy: Multicenter review of outcomes associated with a reconstructive approach
Authors:   Microvascular Committee of the American Academy of Otolaryngology‐Head & Neck Surgery*
Published: December 2018

AbstractBackgroundSurgeons have developed various reconstructive techniques to minimize the rate of pharyngocutaneous fistula and optimize functional outcome after salvage laryngectomy or laryngopharyngectomy.MethodsMulticenter retrospective review at 33 institutions of 486 patients with a history of squamous cell carcinoma (SCC) of the larynx or hypopharynx previously treated with primary chemoradiotherapy (CRT) who required salvage surgery. Outcomes evaluated were overall fistula rate, fistula requiring reoperation, and 12‐month speech and swallowing function.ResultsPrimary closure of the hypopharynx was associated with a statistically higher overall fistula rate and fistula requiring reoperation compared to reconstruction with vascularized tissue augmentation. Vascularized tissue augmentation with muscle led to worse 12‐month “understandability of speech” and “nutritional mode” scores compared to vascularized tissue augmentation without muscle.ConclusionVascularized tissue augmentation reduces the overall fistula rate and fistula requiring reoperation but vascularized tissue augmentation with muscle may impair speech and swallowing outcomes.
Recognizing dissimilarities between resilience engineering and EFQM approaches to ensure safety in hospitals
Authors: Asadzadeh Seyed Mohammad , Tanhaeean Mehrab , Abdi Niloofar 
Published: December 2018

AbstractA common ground between resilience engineering (RE) and European Foundation for Quality Management (EFQM) model is to address safety issues. However, the advent of RE may adversely become inefficient or may be regarded redundant if there is no clear uniqueness with RE. This study aims to achieve a clear distinction between the perspectives of RE and EFQM model to address safety issues, using a questionnaire‐based comparative approach, in a large hospital. The paper contributes to the scientific field by enabling the comparison using an integrated approach of fuzzy analytical hierarchy process (AHP), simulated annealing (SA), and clustering techniques. Fuzzy AHP provides assessment scores of RE and EFQM. The SA identifies the RE items having the least correlation with the EFQM ones. Finally, the identified RE items are categorized by k‐means clustering into a set of unique healthcare‐focused RE factors to help healthcare practitioners have a clear understanding of RE concepts and principles.
River flood seasonality in the Northeast United States: Characterization and trends
Authors: Collins Mathias J. 
Published: December 2018

AbstractThe New England and Mid‐Atlantic regions of the Northeast United States have experienced climate‐induced increases in both the magnitude and frequency of floods. However, a detailed understanding of flood seasonality across these regions, and how flood seasonality may have changed over the instrumental record, has not been established. The annual timing of river floods reflects the flood‐generating mechanisms operating in a basin, and many aquatic and riparian organisms are adapted to flood seasonality, as are human uses of river channels and flood plains. Changes in flood seasonality may indicate changes in flood‐generating mechanisms, and their interactions, with important implications for habitats, flood plain infrastructure, and human communities. I applied a probabilistic method for identifying flood seasons at a monthly resolution for 90 Northeast U.S. watersheds with natural, or near‐natural, flood‐generating conditions. Historical trends in flood seasonality were also investigated. Analyses were based on peaks‐over‐threshold flood records that have, on average, 85 years of data and three peaks per year—thus providing more information about flood seasonality than annual maximums. The results show rich detail about annual flood timing across the region with each site having a unique pattern of monthly flood occurrence. However, a much smaller number of dominant seasonal patterns emerged when contiguous flood‐rich months were classified into commonly recognized seasons (e.g., Mar–May, spring). The dominant seasonal patterns identified by manual classification were corroborated by unsupervised classification methods (i.e., cluster analyses). Trend analyses indicated that the annual timing of flood‐rich seasons has generally not shifted over the period of record, but 65 sites with data from 1941 to 2013 revealed increased numbers of June–October floods—a trend driving previously documented increases in Northeast U.S. flood counts per year. These months have been historically flood‐poor at the sites examined, so warm‐season flood potential has increased with possible implications for aquatic and riparian organisms.
Loss of m6A on FAM134B promotes adipogenesis in porcine adipocytes through m6A‐YTHDF2‐dependent way
Authors: Cai Min , Liu Qing , Jiang Qin , Wu Ruifan , Wang Xinxia , Wang Yizhen 
Published: December 2018

AbstractN6‐methyladenosine (m6A) mRNA modification plays an important role in adipogenesis, but its role on single gene remains unexplored. Family with Sequence Similarity 134, Member B (FAM134B) is a cis‐Golgi transmembrane protein that known to be necessary for the long‐term survival of nociceptive and autonomic ganglion neurons. Recent work has shown that FAM134B plays a pivotal role in lipid homeostasis and was identified as its significant m6A level difference between Chinese local Jinhua pigs and Landrace through RNA‐sequence. Here, we construct the non‐m6A FAM134B coding sequence (CDS) plasmid (FAM134B‐MUT) and found one important m6A site on its CDS. Expression of FAM134B‐MUT was more effective in promoting porcine preadipocytes adipogenic differentiation and lipid deposition than wild‐type FAM134B (FAM134B‐WT) both in early and ultimate differentiation stage. FAM134B‐MUT functions better in promoting fat deposition by upregulating peroxisome proliferator‐activated receptor γ (PPARγ) and CCAAT/enhancer‐binding protein (C/EBPα) level. The m6A reader protein YTH m6A RNA binding protein 2 (YTHDF2) interacts with FAM134B mRNA and down regulated its protein level. These results demonstrate that FAM134B was the target of YTHDF2, which may recognize and binds the m6A site of FAM134B to reduce its mRNA lifetime and reduce its protein abundance. © 2018 IUBMB Life, 71(5):580–586, 2019
Vasculature reconstruction of decellularized liver scaffolds via gelatin‐based re‐endothelialization
Authors: Meng Fanwei , Almohanna Falah , Altuhami Abdullah , Assiri Abdallah M. , Broering Dieter 
Published: December 2018

AbstractDecellularized liver scaffolds based liver engineering is a promising approach toward developing functional liver surrogates. However, a major obstacle to long‐term transplantation is the hemocompatibility of the bioengineered liver surrogates. One approach to improve the hemocompatibility of engineered liver surrogates is re‐endothelialization. In the current study, immortalized endothelial cells were perfused for re‐endothelialization of decellularized rat liver scaffolds. When compared to the media‐based perfusion approach, gelatin hydrogels‐based perfusion significantly increased the number of cells that were retained in the decellularized liver scaffolds and the vascular lumen coverage ratio. Endothelial cells were lining along the vasculatures of the decellularized liver scaffolds and actively proliferating. Re‐endothelialization improved the blood retention ability of the liver scaffold vasculatures. Doppler ultrasound detected active blood flows within the re‐endothelialized liver scaffold transplants 8 days post‐transplantation. Our results strengthened the feasibility of developing bioengineered liver surrogates utilizing decellularized liver scaffolds. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 392–402, 2019.
Fabrication and characterization of the 3D‐printed polycaprolactone/fish bone extract scaffolds for bone tissue regeneration
Authors: Heo Seong‐Yeong , Ko Seok‐Chun , Oh Gun‐Woo , Kim Namwon , Choi Il‐Whan , Park Won Sun , Jung Won‐Kyo 
Published: December 2018

AbstractFish bone extract (FBE) containing a trioligopeptide (FBP‐KSA, Lys‐Ser‐Ala) isolated fromJohnius belengeriicould induce osteogenic activities on MC3T3‐E1 pre‐osteoblasts in our previous study. Regarding the osteogenic effect of FBE, in the present study, we fabricated the three‐dimensional (3D) interconnected polycaprolactone (PCL)/FBE scaffolds for bone tissue regeneration. After fabrication of PCL scaffolds using 3D printing, FBE was coated on the surface of PCL scaffolds by self‐assembly process. In the physical characteristic and mechanical property tests, the results demonstrated that the fabricated scaffolds have the strut diameter (between 340 and 345 μm), pore size (between 470 and 480 μm), porosity (between 50% and 55%), and tensile properties (Young's modulus: 9.18–9.42 MPa; max tensile strengths 82.3–87.4 MPa) were similar to those of PCL scaffold. In the cell proliferation and osteogenic assay, the results showed that PCL/FBE scaffolds could significantly induce cell proliferation, calcium deposition, and the expression of osteogenic phenotype markers such as alkaline phosphatase, osteopontin, osteocalcin, and bone morphogenetic protein‐2 in the osteoblasts. These results suggest that FBE‐coated PCL scaffolds are promising materials for use in biomedical application to promote bone tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1937–1944, 2019.
Bone as a Preferential Site for Metastasis
Authors: Sowder Miranda E , Johnson Rachelle W 
Published: December 2018

ABSTRACTBone marrow provides a unique microenvironment favoring the colonization and outgrowth of metastatic tumor cells. Despite the high incidence of bone metastasis in breast and prostate cancer patients, many of the molecular mechanisms controlling metastatic progression remain unclear. Several gene signatures associated with bone metastasis have been reported, but no metastasis‐specific gene alterations have been identified. Therefore, there has been considerable interest in understanding how the bone microenvironment impacts the behavior of disseminated tumor cells (DTCs) prior to and following colonization of the bone. Substantial evidence indicates that disruption of normal bone homeostasis by tumor‐derived factors establishes a premetastatic niche within the bone that favors DTC colonization. Following dissemination, bone resident cells and the surrounding stroma provide critical signals that support tumor cell colonization, survival, and eventual outgrowth. Clinical data suggest that patients can harbor DTCs for years to decades prior to developing overt bone metastases, suggesting a period of tumor dormancy occurs in the bone marrow. Several dormancy‐promoting factors have been recently identified; however, critical questions surrounding the molecular triggers and timing of tumor cell emergence from dormancy remain. Here, we review how metastatic tumor cells co‐opt the bone marrow microenvironment for metastatic progression and discuss emerging insights into how to more effectively target DTCs and prevent metastasis. © 2018 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research
Predictors of Imminent Risk of Nonvertebral Fracture in Older, High‐Risk Women: The Framingham Osteoporosis Study
Authors: Hannan Marian T , Weycker Derek , McLean Robert R , Sahni Shivani , Bornheimer Rebecca , Barron Richard , Travison Thomas G , Kiel Douglas P 
Published: December 2018

AbstractOsteoporosis treatment decisions are often based solely on BMD or on 10‐year fracture risk; little is known about factors increasing imminent fracture risk. Understanding factors contributing to imminent risk of fracture is potentially useful for personalizing therapy, especially among those at high risk. Our aim was to identify predictors of nonvertebral fracture for 1‐ and 2‐year periods in women at high risk for fracture. The Framingham Osteoporosis Study cohort included 1470 women (contributing 2778 observations), aged ≥65 years with BMD hip T‐score ≤ −1.0, or history of fragility fracture (irrespective of T‐score). Nonvertebral fractures were ascertained prospectively over 1 year and 2 years following a baseline BMD scan. Potential risk factors included age, anthropometric variables, comorbidities/medical history, cognitive function, medications, history of fracture, self‐rated health, falls in the past year, smoking, physical performance, hip BMD T‐score, Activities of Daily Living (ADL) score, and caffeine and alcohol intakes. Predictive factors with p value ≤ 0.10 in bivariate Cox proportional hazards regression models were subsequently considered in multivariable models. Mean baseline age was 75 years (SD 6.0). During 1‐year follow‐up, 89 nonvertebral fractures occurred; during 2‐year follow‐up, 176 fractures occurred. Of the variables considered in the bivariate models, significant predictors of nonvertebral fractures included age, history of fracture, self‐rated health, falls in the prior year, BMD T‐score, ADL, renal disease, dementia, and current use of nitrates, beta‐blockers, calcium channel blockers, or antidepressants. In multivariable models, significant independent risk factors were history of fracture, self‐rated health, hip BMD T‐score, and use of nitrates. Significant 1‐year results were attenuated at the 2‐year follow‐up. In addition to the traditional factors of BMD and fracture history, self‐rated health and use of nitrates were independently associated with imminent risk of fracture in older, high‐risk women. These specific risk factors thus may be useful in identifying which women to target for therapy. ©2018 The Authors JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.