bluuebunny/crossref_metadata_2025_split huggingface dataset:
Descemet’s Membrane Endothelial Keratoplasty for Pseudoexfoliation Syndrome: A Case Series
Authors: Tase Saho , Shimizu Toshiki , Hayashi Takahiko , Tabuchi Hitoshi , Niimi Koji , Mizuki Nobuhisa , Kato Naoko 
Published: May 2019

Abstract
Bachground: To evaluate the clinical outcomes and features of Descemet’s membrane endothelial keratoplasty (DMEK) for eyes with pseudoexfoliation syndrome (PEX).

Methods: In this retrospective study, 37 DMEK cases were reviewed from available medical records. Patients who exhibited endothelial dysfunction derived from PEX or Fuchs endothelial corneal dystrophy (FECD) and successfully underwent cataract surgery about four weeks before DMEK were enrolled. The best spectacle-corrected visual acuity (BSCVA), central corneal thickness (CCT), endothelial cell density (ECD), and incidence of intra-operative/post-operative complications of DMEK were analyzed.

Results: This study included 14 eyes of 14 patients (PEX: n=6, FECD: n=8). There was no primary graft failure. In the PEX group, BSCVA improved from 0.67 ± 0.28 at the preoperative point to 0.43 ± 0.14 at 1 month, 0.27 ± 0.10 at 3 months, and 0.19 ± 0.08 at 6 months after DMEK. The donor corneal ECD was 2,704 ± 225 cells/mm2 at the preoperative point and decreased to 1,691 ± 498 cells/mm2 at 1 month, 1,425 ± 366 cells/mm2 at 3 months, and 1,281 ± 340 cells/mm2 (52.7 ± 11.7% less than ECD of the donor graft) at 6 months after DMEK. None of the patients required rebubbling. When compared with the FECD group, no statistical difference was observed in CCT (p=0.821); BSCVA (p=0.001) and the reduction rate of ECD (p=0.010) were relatively worse.

Conclusions: DMEK is effective for the treatment of endothelial dysfunction due to PEX.
Association between dynamic obesity and mortality in patients with first-ever ischemic stroke: a hospital-based prospective study
Authors: Wang Ju , Li Jijie , Li Mier , Hou Lisha , Zhu Ping , Du Xudong , Zhou Muke , Zhu Cairong 
Published: May 2019

Abstract
Background and Purpose: Although obesity is an established risk factor for the occurrence of a primary stroke, the association between obesity with post-stroke mortality remains unclear. We evaluated the association of dynamic obesity status with mortality among first-ever stroke survivors in China.
Methods: Data from 775 patients with first-ever ischaemic stroke from a longitudinal study, 754 patients were categorized 4 categories of BMI (underweight, normal weight, overweight and obese) and 2 categories of WC (normal WC and abdominal obesity) according to Chinese-specific criteria. Mortality information and obesity status were obtained through every 3 months via telephone follow-up, beginning in 2010 and continuing through 2016. Chi-square tests were used to compare different obesity status at admission and personal characteristics. Time-dependent Cox proportional hazards models were used to estimate the unadjusted and adjusted hazard ratios (HRs) for relationship between all-cause mortality and dynamic obesity status.
Results: Of 754 analyzed patients, 60.87% were males, with a mean age of 61.45 years. After adjusting for possible confounders, there were significant inverse associations between BMI and WC with all-cause mortality: compared with normal BMI or WC counterparts, overweight and abdominal obesity patients significantly decrease risk of all-cause mortality (HR and 95% confidence interval (CI):0.521(0.303,0.897),0.545(0.352,0.845),respectively), whereas under-weight patients have the highest risk and obese have decreased risk of mortality, though significance was not obtained (1.241(0.691,2.226),0.486(0.192,1.231), respectively).
Conclusions: Overweight and abdominal obesity was paradoxically associated with reduced risk of mortality among first-ever ischaemic stroke survivors in China. Body weight management recommendations ought to not be based on mere projection from primary prevention of stroke.
Integrated analysis of small RNA, transcriptome and degradome sequencing provides new insights into floral development and abscission in yellow lupine (Lupines luteus L.)
Authors: Glazinska Paulina , Kulasek Milena , Glinkowski Wojciech , Wojciechowski Waldemar , Kosiński Jan 
Published: May 2019

Abstract
Background

Yellow lupine (Lupinus luteus L., Taper c.) is an important legume crop. However, its flower development and pod formation are often affected by excessive abscission. Organ detachment occurs within the abscission zone (AZ) and in L. luteus primarily affects flowers formed at the top of the inflorescence. The top flowers’ fate appears determined before anthesis. The organ development and abscission mechanisms utilize a complex molecular network, not yet not fully understood, especially as to the role of miRNAs and siRNAs. We aimed at identifying differentially expressed (DE) small ncRNAs in lupine by comparing small RNA-seq libraries generated from developing upper and lower raceme flowers, and flower pedicels with active and inactive AZs. Their target genes were also identified using transcriptome and degradome sequencing.

Results

Within all the libraries, 394 known and 28 novel miRNAs and 316 phased siRNAs were identified. In flowers at different stages of development, 30 miRNAs displayed DE in the upper and 29 in the lower parts of the raceme. In comparisons between upper and lower raceme flowers, a total of 46 DE miRNAs were identified. miR393 and miR160 were related to the upper and miR396 to the lower flowers and pedicels of non-abscising flowers. In flower pedicels we identified 34 DE miRNAs, with miR167 being the most abundant of all. Most siRNAs seem to play a marginal role in the processes studied herein, with the exception of tasiR-ARFs, which were DE in the developing flowers. The target genes of these miRNAs were predominantly categorized into the following Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways: ‘Metabolism’ (15,856), ‘Genetic information processing’ (5,267), and ‘Environmental information processing’ (1,517). Over 700 putative targets were categorized as belonging to the ‘Plant Hormone Signal Transduction pathway’. 26,230 target genes exhibited Gene Ontology (GO) terms: 23,092 genes were categorized into the ‘Cellular component’, 23,501 into the ‘Molecular function’, and 22,939 into the ’Biological process’.

Conclusion

Our results indicate that miRNAs and siRNAs in yellow lupine may influence the development of flowers and, consequently, their fate by repressing their target genes, particularly those associated with the homeostasis of phytohormones, mainly auxins.
Transcriptome and expression analysis of Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) in response to cycloxaprid
Authors: Xue Jin Jian , Zhao-Chun Ye , Ying Cheng , Feng-Liang Li , Wen-Hong Li , Yu-Hang Zhou , Dao-Chao Jin 
Published: May 2019

Abstract
Background

The white backed planthopper, Sogatella furcifera (Horváth), causes substantial damage to many crops by direct feeding or transmitting plant viruses, especially southern rice black-streaked dwarf virus (SRBSDV) that has become a great threat to rice production in East Asia. Cycloxaprid, a novel cis-nitromethylene neonicotinoid insecticide, has good industrialization prospects because of its high efficiency against rice planthoppers, including imidacloprid-resistant populations. This chemical would be used extensively in future. However, very little is known about the influence on S. furcifera after cycloxaprid application at the molecular level. We sequenced S. furcifera transcriptome of female adult of S. furcifera using the Illumina sequencing.

Results

By de novo transcriptome assembling and massive parallel pyrosequencing, we constructed two transcriptomes of S. furcifera and profiled the alternation of gene expression in response to cycloxaprid treatment in transcriptional level. Over 157,906,456 nucleotides and 131,601 different unigenes were generated using Illumina technology from both cycloxaprid-treatment and no-treatment S. furcifera. And a total of 38,534 unigenes matched known proteins in at least one database, accounting for 29.28% in total unigenes. The number of Coding DNA Sequence (CDS) were 28,546 and that of the amino acid sequence of coding region were 22,299. A total 15,868 simple sequence repeats (SSRs) were identified. The trinucleotide repeats accounted for 45.1% (7,157) in total SSRs and the (AAG/CTT)n was the most frequent motif. There were 359 differentially expressed genes (DEGs) that might be induced by cycloxaprid. There were 131 genes up-regulated and 228 down-regulated. 22 unigenes may take participate in the resistance to cycloxaprid, such as cytochrome P450, Glutathione-s transferase (GST), Acid phosphatase (ACP), cadherin, etc.

Conclusions

Our study will provide a splendid database for future investigations of the resistance mechanism induced by cycloxaprid and will provide new strategies for pest management and crop protection.
Epidemiology and molecular characterization of Enterobacteriaceae producing Extended spectrum β-lactamase in extensive and extensive breeding animals in Burkina Faso
Authors: SANOU Soufiane , Ouedraogo Abdoul Salam , Lounnas Manon , Zougmore Arnaud , Poda Armel , Zoungrana Jacques , Ouedraogo Georges Anicet , Traore/Ouedraogo Rasmata , Ouchar Oumar , Carriere Christian , Pierre Helene Jean - , Godreuil Sylvain 
Published: May 2019

Abstract
Summary

Background

Antimicrobial resistance genes can be found in all ecosystems even in those where antibiotic selection pressure is less exerted. Extended spectrum 𝛽-lactamases (ESBL) determinants have been detected in clinical isolates and commensal bacteria from humans and animals. Here we investigated, for the first time, the ESBL-producing Enterobacteriaceae in stool from intensive and extensive breeding animals (cattle, pigs and poultry) in Burkina Faso.

Results

from March to June 2017 in the Bobo Dioulasso area, we investigated stool samples collected from healthy animals (cattle = 251; pigs = 250 and poultry = 397) in one (1) slaughterhouse, five (5) livestock farms and one (1) poultry market. The frequency of ESBL genes carriage was 41.03% among cattle, 69.60% among pigs, 0.8% among intensive farming and 19.1% among extensive poultry farming. Only all the poultry were fed with antibiotics. The bacterial strains carrying the ESBL were E. coli (278/315) and K. pneumoniae (36/315). The ESBL genes carried were CTX-M 15, TEM and Oxa-1-like. These three β- lactamase genes were associated in some bacterial strains. The E. coli strains belonged most commonly to the phylogroup A.

Conclusion

This study showed the high resistance of Enterobacteriaceae to antibiotics in livestock in Burkina Faso by production of ESBL. This high level of resistance was observed in animals that did not receive antibiotics. This situation could suggest an environmental contamination of the livestock with ESBL-producing bacteria.
Cell-based Therapy for Perianal Fistulising Crohn’s Disease
Authors: Kotze Paulo Gustavo , Spinelli Antonino , Lightner Amy Lee 
Published: May 2019

Background:
The management of complex perianal fistulas in Crohn’s disease (CD) represents a
challenge for patients, gastroenterologists and colorectal surgeons. There are clear limitations with current medical
and surgical options, and healing rates remain far from what is expected. A multidisciplinary approach with
optimized medical therapy, usually anti-TNF agents, associated with setons and additional surgical techniques is
currently the best strategy to aim fistula healing.

Methods:
A comprehensive review of the literature was conducted on the use of mesenchymal stem cells
(MSCs).

Results:
The use of mesenchymal stem cells (MSCs) has recently emerged as a promising new therapeutic strategy
for complex fistulas in CD patients. This review summarizes the evidence of the use of MSCs in complex CD
fistulas, by exploring in detail the types of cells that can be used and their modes of delivery. Additionally, the
results of the most recent phase III randomized trial with local MSCs injection are described, and future challenges
of this therapeutic option are discussed.

Conclusion:
The use of MSCs represents hope for better outcomes in patients with CD-related perianal fistulas.
More research in the field will help to position this specific therapy in treatment algorithms.
Diagnostic Value of Non-Invasive Prenatal Screening of β-thalassemia by Cell Free Fetal DNA and Fetal NRBC
Authors: Shafei Nadia , Hakhamaneshi Mohammad Saeed , Houshmand Massoud , Gerayeshnejad Siavash , Fathi Fardin , Sharifzadeh Sardar 
Published: May 2019

Background:
Beta thalassemia is a common disorder with autosomal
recessive inheritance. The most prenatal diagnostic methods are the invasive
techniques that have the risk of miscarriage. Now the non-invasive methods will be
gradually alternative for these invasive techniques.

Objective:
The aim of this study is to evaluate and compare the diagnostic value of two
non-invasive diagnostic methods for fetal thalassemia using cell free fetal DNA (cff-DNA)
and nucleated RBC (NRBC) in one sampling community.

Methods:
10 ml of blood was taken in two k3EDTA tube from 32 pregnant women
(mean of gestational age = 11 weeks), who themselves and their husbands had minor
thalassemia. One tube was used to enrich NRBC and other was used for cff-DNA
extraction. NRBCs were isolated by MACS method and immunohistochemistry; the
genome of stained cells was amplified by multiple displacement amplification (MDA)
procedure. These products were used as template in b-globin segments PCR. cff-DNA
was extracted by THP method and 300 bp areas were recovered from the agarose gel
as fetus DNA. These DNA were used as template in touch down PCR to amplify b-globin
gen. The amplified b-globin segments were sequenced and the results compared with
CVS resul.

Results:
The data showed that sensitivity and specificity of thalassemia diagnosis by
NRBC were 100% and 92% respectively and sensitivity and specificity of thalassemia
diagnosis by cff-DNA were 100% and 84% respectively.

Conclusion:
These methods with high sensitivity can be used as screening test but due
to their lower specificity than CVS, they cannot be used as diagnostic test.
Repurposing Disulfiram as An Anti-Cancer Agent: Updated Review on Literature and Patents
Authors: Ekinci Elmira , Rohondia Sagar , Khan Raheel , Dou Qingping P. 
Published: May 2019

Background:Despite years of success of most anti-cancer drugs, one of the major clinical problems is inherent and acquired resistance to these drugs. Overcoming the drug resistance or developing new drugs would offer promising strategies in cancer treatment. Disulfiram, a drug currently used in the treatment of chronic alcoholism, has been found to have anti-cancer activity.Objective:To summarize the anti-cancer effects of Disulfiram through a thorough patent review.Methods:This article reviews molecular mechanisms and recent patents of Disulfiram in cancer therapy.Results:Several anti-cancer mechanisms of Disulfiram have been proposed, including triggering oxidative stress by the generation of reactive oxygen species, inhibition of the superoxide dismutase activity, suppression of the ubiquitin-proteasome system, and activation of the mitogen-activated protein kinase pathway. In addition, Disulfiram can reverse the resistance to chemotherapeutic drugs by inhibiting the P-glycoprotein multidrug efflux pump and suppressing the activation of NF-kB, both of which play an important role in the development of drug resistance. Furthermore, Disulfiram has been found to reduce angiogenesis because of its metal chelating properties as well as its ability to inactivate Cu/Zn superoxide dismutase and matrix metalloproteinases. Disulfiram has also been shown to inhibit the proteasomes, DNA topoisomerases, DNA methyltransferase, glutathione S-transferase P1, and O6- methylguanine DNA methyltransferase, a DNA repair protein highly expressed in brain tumors. The patents described in this review demonstrate that Disulfiram is useful as an anti-cancer drug.Conclusion:For years the FDA-approved, well-tolerated, inexpensive, orally-administered drug Disulfiram was used in the treatment of chronic alcoholism, but it has recently demonstrated anti-cancer effects in a range of solid and hematological malignancies. Its combination with copper at clinically relevant concentrations might overcome the resistance of many anti-cancer drugs in vitro, in vivo, and in patients.
Influence of Worktable Errors on the Pascal Limacon Gear Pitch Curve
Authors: Wang Yazhou , Liu Yongping 
Published: May 2019

Background:
More and more noncircular gears are reported in various relevant patents and
papers. The Pascal limacon gear is one kind of noncircular gears and widely used in many mechanisms.
The pitch curve is a key accuracy index for the noncircular gears and influenced by many errors, included
worktable errors.

Objective:
The goal of this paper is to analyze the influence of worktable errors on the Pascal limacon
gear’s pitch curve.

Methods:
Firstly, according to the mathematical model for hobbing Pascal limacon gear, a vector expression
for the Pascal limacon gear pitch curve errors caused by the worktable rotary angle error and
the center distance error was derived. Then, with consideration of the above errors, a comprehensive
method was proposed, and the Pascal limacon gear pitch curve errors were calculated. The concept of
the sensitivity degree of the errors of the Pascal limacon gear pitch curve to the center distance was
proposed. Thirdly, the influence of the center distance errors on the pitch curve could be assessed.

Results:
Using one Pascal limacon gear as an example, the results show that the worktable rotary angle
error has a slight effect on the Pascal limacon gear pitch curve, and the center distance error has a great
effect on the Pascal limacon gear pitch curve.

Conclusion:
The Pascal limacon gear pitch curve error is highly sensitive to the center distance error near the polar angles of π/ 2 and 3π / 2.
Cooperación internacional y educación superior indígena en América Latina: constitución de un campo de acción
Authors: Didou Aupetit Sylvie 
Published: May 2019

La presencia de organismos y agencias de cooperación internacional o bilateral en el campo de la educación superior indígena se ha incrementado en la década pasada, en América Latina. Esos organismos auspiciaron la institucionalización de dispositivos de atención y generaron capacidades innovadoras de gestión, atención pedagógica e investigación. Tomando en cuenta la escasa información sistematizada sobre su rol, sus incidencias y sus alianzas con organismos nacionales con propósitos convergentes, caracterizaremos, con base en ejemplos, sus características, áreas de incidencia y propósitos en términos de inclusión, equidad y respecto a la diversidad.
Resultados más significativos del posgrado en una universidad cubana. Perspectivas del proceso desde el contexto del municipio.
Authors: De la Cruz Soriano Raquel , Boullosa Torrecilla Armando , Guevara Reyes Onelia 
Published: May 2019

El presente trabajo constituyó un aporte a la gestión del posgrado en una universidad territorial, donde se concibió como un proceso cíclico para hacer efectivo y pertinente la gestión, en el se incluyeron las etapas siguientes: caracterización de las entidades y organismos priorizados del territorio, cuantificación del potencial de profesionales, determinación de necesidades de formación posgraduada, concepción del programa de posgrado y ejecución del programa, evaluación de impacto del programa de posgrado y una retroalimentación continua con las necesidades de formación posgraduada.A partir de los resultados obtenidos en el diagnóstico de las necesidades de superación posgraduada y del impacto de la actividad posgraduada realizadas en el periodo de 1996-1998; lo cual constituyó una notable contribución a la gestión del posgrado en el territorio en la etapa posterior y al establecimiento de un procedimiento para la determinación de necesidades de superación posgraduada.Los mayores impactos demostraron el comportamiento satisfactorio de los indicadores de profesionales superados en diferentes figuras de posgrado; se incluyeron, los doctorados, maestrías, especialidades, diplomados, cursos y entrenamientos. Además cualitativamente se han experimentado mejorías en las acciones de posgrado que han abarcado diferentes perfiles profesionales y problemáticas de interés para el territorio.Dadas las transformaciones en la proyección del posgrado, se contextualizó este proceso en las condiciones concretas de los municipios, desde las perspectivas de las dimensiones de la determinación de necesidades de formación de los profesionales.
Los fundamentos pedagógicos que sustenta la práctica docente de los profesores que ingresan y egresan de la Maestría en Intervención Socioeducativa
Authors: Martínez Camacho Margarita 
Published: May 2019

La presente investigación se enfoca en los procesos de transformación conceptual que ocurren en el aprendizaje de los alumnos del nivel de posgrado; se analizó la conceptualización pedagógica que poseen los estudiantes de la maestría en Intervención Socioeducativa, al ingresar y al estar por egresar, con el propósito de observar diferencias significativas sobre su formación inicial, experiencia profesional y formación continua. La investigación se desarrolló por medio de la metodología mixta, la fenomenología, el método interpretativo y el estudio de casos, a través de encuestas, entrevistas y registros de observación, y del análisis estadístico. Los hallazgos encontrados evidencian que los alumnos de 1° semestre de maestría reflejan en su concepción un enfoque tradicionalista y conductista más arraigado que los de 5°, esto significa que no ha existido una transformación o cambio conceptual a fondo y de forma consciente, por lo que existe una evolución conceptual pedagógica a partir de sus estudios de maestría.
El aprendizaje de la química en los nuevos “Laboratorios de Ciencias para el Bachillerato UNAM”
Authors: Sánchez-Lazo Pérez Sheila , Gallegos Cázares Leticia , Flores Camacho Fernando 
Published: May 2019

Este trabajo muestra las posibilidades en la mejora del aprendizaje de temas científicos – enlace químico– en el bachillerato con la aplicación de una secuencia didáctica orientada desde una perspectiva educativa basada en la multi representacionalidad, la colaboración y el empleo de las técnica de la información y la comunicación (TIC) en un entorno como el que presentan los nuevos laboratorios de ciencias del bachillerato de la Universidad Nacional Autónoma de México (UNAM). Los resultados muestran que los estudiantes participantes, en un porcentaje significativo, integran diversas representaciones y, con ello, explican los modelos del enlace químico, las propiedades, estructura y composición de los materiales desde el nivel nanoscópico, a diferencia de estudiantes que cursan la asignatura de forma tradicional.
Los estudiantes universitarios con diversidad funcional visual. Sus retos
Authors: Márquez Ramírez Genoveva 
Published: May 2019

Este artículo plantea algunos conceptos y resultados encontrados durante la investigación sobre resiliencia en los universitarios con diversidad funcional: con ceguera parcial o total. El aporte de nuestro trabajo consiste en responder a la interrogante sobre las experiencias que han vivido los estudiantes con diversidad funcional (visual) de la Universidad Autónoma de Tlaxcala, México, para responder a las necesidades y expectativas que les genera el ámbito académico. A lo largo de la historia de la humanidad, el término discapacidad (diversidad funcional ahora) ha estado presente. En México, algunos esfuerzos conjuntos han llegado a promover cambios de actitud en la sociedad, los gobiernos y los centroseducativos. Además, el tratamiento se debe proporcionar a las personas que lo necesitan.