bluuebunny/crossref_metadata_2025_split huggingface dataset:
A screening questionnaire for occupational and hobby exposures during pregnancy
Authors: Zachek C M , Schwartz J M , Glasser M , DeMicco E , Woodruff T J 
Published: June 2019

Abstract

Background
Occupational and environmental exposures during the prenatal period may be associated with adverse pregnancy outcomes and lifelong health effects. Yet, identification and evaluation of these potential hazards is lacking in routine obstetric care.

Aims
To assess the feasibility of incorporating a self-administered occupational and environmental exposure questionnaire into obstetric clinics.

Methods
A cross-sectional survey assessed prenatal clinic patients at a public hospital who were currently employed and &lt;20 weeks gestation. Questionnaires evaluated job characteristics, workplace and hobby exposures, protective equipment use and symptoms during pregnancy.

Results
Of 69 participants (96% response rate), 46% were predominantly Spanish-speaking. Primary occupations were caregiver (16%), cleaner (14%) and administrative assistant (14%). Overall, 93% were exposed to a workplace hazard, with most participants reporting physical stressors (82%) or organic solvent exposure (78%). Most women (74%) used some personal protective equipment. Nearly half (54%) reported at least one non-pregnancy symptom, and 52% were referred for follow-up with an occupational medicine practitioner. Household and hobby-related chemical exposures were common in our sample (91%). We observed moderate consistency between job task and chemical use responses: 67–99% of intentionally redundant questions were fully or partially matched. Closed- compared to open-ended activity questions identified a higher proportion of physical stressors (82% versus 12%) and cleaning product (76% versus 30%) exposures.

Conclusions
A self-administered questionnaire is an effective screening tool for identifying women with occupational and hobby-related exposures during pregnancy. Consistent incorporation of exposure assessment into prenatal care can improve clinical communications and early interventions for at-risk pregnant women.
Interest in Switching to On-Demand HIV Pre-Exposure Prophylaxis (PrEP) Among Australian Users of Daily PrEP: An Online Survey
Authors: Cornelisse Vincent J , Lal Luxi , Price Brian , Ryan Kathleen E , Bell Charlotte , Owen Louise , Wright Edwina J 
Published: June 2019

AbstractWe surveyed 970 PrEPX study participants to evaluate interest in switching from daily to on-demand PrEP in a study setting. Interested respondents (n = 469, 48%) more commonly reported PrEP cessation (adjusted odds ratio [aOR], 3.0; P &lt; .001), difficulty with adherence (aOR, 1.6; P = .029), infrequent sex (aOR, 3.7; P &lt; .001), and toxicity concerns (aOR, 2.7; P &lt; .001).
Boötes. IV. A new Milky Way satellite discovered in the Subaru Hyper Suprime-Cam Survey and implications for the missing satellite problem
Authors: Homma Daisuke , Chiba Masashi , Komiyama Yutaka , Tanaka Masayuki , Okamoto Sakurako , Tanaka Mikito , Ishigaki Miho N , Hayashi Kohei , Arimoto Nobuo , Carlsten Scott G , Lupton Robert H , Strauss Michael A , Miyazaki Satoshi , Torrealba Gabriel , Wang Shiang-Yu , Murayama Hitoshi 
Published: June 2019

AbstractWe report on the discovery of a new Milky Way (MW) satellite in Boötes based on data from the ongoing Hyper Suprime-Cam (HSC) Subaru Strategic Program (SSP). This satellite, named Boötes IV, is the third ultra-faint dwarf that we have discovered in the HSC-SSP. We have identified a statistically significant (32.3σ) overdensity of stars with characteristics of a metal-poor, old stellar population. The distance to this stellar system is $D_{\odot }=209^{+20}_{-18}\:$kpc with a V-band absolute magnitude of $M_V=-4.53^{+0.23}_{-0.21}\:$mag. Boötes IV has a half-light radius of $r_{\rm h}=462^{+98}_{-84}\:$pc and an ellipticity of $0.64^{+0.05}_{-0.05}$, which clearly suggests that this is a dwarf satellite galaxy. We also found another overdensity that appears to be a faint globular cluster with $M_V=-0.20^{+0.59}_{-0.83}\:$mag and $r_{\rm h}=5.9^{+1.5}_{-1.3}\:$pc located at $D_{\odot }=46^{+4}_{-4}\:$kpc. Adopting the recent prediction for the total population of satellites in a MW-sized halo by Newton et al. (2018, MNRAS, 479, 2853), which combined the characteristics of the satellites observed by the Sloan Digital Sky Survey and the Dark Energy Survey with the subhalos obtained in ΛCDM models, we estimate that there should be about two MW satellites at MV ≤ 0 in the ∼676 deg2 covered by HSC-SSP, whereas that area includes six satellites (Sextans, Leo IV, Pegasus III, Cetus III, Virgo I, and Boötes IV). Thus, the observed number of satellites is larger than the theoretical prediction. On the face of it, we have a problem of too many satellites, instead of the well-known missing satellites problem whereby the ΛCDM theory overpredicts the number of satellites in a MW-sized halo. This may imply that the models need more refinement for the assignment of subhalos to satellites, such as considering those found by the current deeper survey. More statistically robust constraints on this issue will be brought by further surveys of HSC-SSP over the planned ∼1400 deg2 area.
Normal fecal calprotectin levels in healthy children are higher than in adults and decrease with age
Authors: Velasco Rodríguez-Belvís Marta , Viada Bris Javier Francisco , Plata Fernández Carmen , García-Salido Alberto , Asensio Antón Julia , Domínguez Ortega Gloria , Muñoz Codoceo Rosa Ana 
Published: June 2019

AbstractBackground/ObjectivesThe paediatric reference range of fecal calprotectin (FC) has not been decisively established and previous studies show a wide within-age variability, suggesting that other factors like anthropometric data or type of feeding can influence FC. Our aims were to establish the normal levels of FC in healthy children grouped by age and analyze whether sex, gestational age, birth weight, type of delivery, type of feeding, or anthropometric data influence FC values.MethodsThis multicentre, cross-sectional, and observational study enrolled healthy donors under 18 years of age who attended their Primary Health Care Centre for their routine Healthy Child Program visits. The exclusion criteria were: (i) immunodeficiency, (ii) autoimmune or (iii) gastrointestinal disease; (iv) medication usage; (v) gastrointestinal symptoms; or (vi) positive finding in the microbiological study.ResultsWe enrolled 395 subjects, mean age was 4.2 years (range 3 days to 16.9 years), and 204 were male. The median FC was 77.0 mcg/g (interquartile range 246). A negative correlation between age and FC was observed (Spearman’s rho = −0.603, P&lt;0.01), and none of the other factors analyzed were found to influence FC levels.ConclusionsNormal FC values in healthy children (particularly in infants) are higher than those considered to be altered in adults and show a negative correlation with age. It is necessary to reconsider the upper limits of FC levels for paediatric patients according to age, with further studies required to determine other factors that influence FC during infancy.
Bifurcation and anomalous spectral accumulation in an oval billiard
Authors: Makino Hironori 
Published: June 2019

Abstract
The spectral statistics of a quantum oval billiard whose classical dynamical system shows bifurcations is numerically investigated in terms of the two-point correlation function (TPCF), which is defined as the probability density of finding two levels at a specific energy interval. The eigenenergy levels at the bifurcation point are found to show anomalous accumulation, which is observed as a periodic spike oscillation of the TPCF. We analyzed the eigenfunctions localizing onto the various classical trajectories in the phase space and found that the oscillation is supplied from a limited region in the phase space that contains the bifurcating orbit. We also show that the period of the oscillation is in good agreement with the period of a contribution from the bifurcating orbit to the semiclassical TPCF obtained by Gutzwiller’s trace formula [J. Math. Phys. 12, 343 (1971)].
${\bf I=2} \boldsymbol{\pi\pi}$ potential in the HAL QCD method with all-to-all propagators
Authors: Akahoshi Yutaro , Aoki Sinya , Aoyama Tatsumi , Doi Takumi , Miyamoto Takaya , Sasaki Kenji 
Published: June 2019

AbstractIn this paper, we perform the first application of the hybrid method (exact low modes plus stochastically estimated high modes) for all-to-all propagators to the HAL QCD method. We calculate the HAL QCD potentials in the $I=2$$\pi\pi$ scattering in order to see how statistical fluctuations of the potential behave under the hybrid method. All of the calculations are performed with the 2+1 flavor gauge configurations on a $16^3 \times 32$ lattice at the lattice spacing $a \approx 0.12$ fm and $m_{\pi} \approx 870$ MeV. It is revealed that statistical errors for the potential are enhanced by stochastic noises introduced by the hybrid method, which, however, are shown to be reduced by increasing the level of dilutions, in particular, that of space dilutions. From systematic studies, we obtain a guiding principle for a choice of dilution types/levels and a number of eigenvectors to reduce noise contamination to the potential while keeping numerical costs reasonable. We also confirm that we can obtain the scattering phase shifts for the $I=2$$\pi\pi$ system by the hybrid method within a reasonable numerical cost; these phase shifts are consistent with the result obtained with the conventional method. The knowledge that we obtain in this study will become useful for the investigation of hadron resonances that require quark annihilation diagrams such as the $\rho$ meson by the HAL QCD potential with the hybrid method.
Lethal coagulopathy resulting from the consumption of contaminated synthetic cannabinoids: the story of a public health crisis
Authors: Fasih Anum 
Published: June 2019

Abstract
In March 2018, Illinois saw the outbreak of an unprecedented public health crisis, which manifested as the development of a potentially lethal coagulopathy associated with the consumption of synthetic cannabinoids. This outbreak impacted a reported 174 patients, and was responsible for the deaths of five patients. While events unfolded, it was uncovered that the coagulopathy was not caused directly by the consumption of synthetic cannabinoids, but by the contamination of these products with Brodifacuom, bromadiol and difenacoum, potent Vitamin K antagonists that are the active ingredient in most rodenticides. This article chronicles the first reported instance of clinically significant Long Acting Anticoagulant Rodenticide poisoning on such a widespread and uniform scale, and examines the challenges posed to the providers and Public Health authorities tasked with the management of this outbreak. Crisis preparedness determines mitigation, response and recovery during trying times. It is prudent that healthcare agencies engage in collective measures to establish preparedness plans and build up their capacity to respond to health-related crises such as the one faced by Illinois in early 2018. By examining the solutions developed to tackle each unique challenge, the objective of this article is to lay a framework for action and response to similar public health crises.
S136 REPROGRAMMING HEMATOPOIETIC STEM CELL FUNCTION VIA MODULATION OF MITOCHONDRIAL ACTIVITY
Authors: Girotra M. , Rincon‐Restrepo M. , Oggier A. , Coukos G. , Naveiras O. , Rezzi S. , Vannini N. 
Published: June 2019

Background:Hematopoietic stem cells (HSCs) are a small group of cells that ensure a steady production of all mature blood cells throughout an organism's lifetime. HSCs mediate this process by giving rise to progenitors that in turn produce terminally differentiated mature blood cell lineages via several rounds of cell division and well‐orchestrated differentiation steps. Cellular metabolism has emerged as a crucial regulator guiding this complex process. Especially, HSCs differ from their committed progeny by relying primarily on anaerobic glycolysis rather than mitochondrial oxidative phosphorylation for energy production. This distinct metabolic state protects the HSCs from cellular damage inflicted by reactive oxygen species (ROS) in active mitochondria. However, whether this change in the metabolic program is the cause or a consequence of the unique function of HSCs remains unknown.Aims:Having previously shown that mitochondrial activity can be used as a reliable readout for HSC fate, here we asked if modulation of mitochondrial activity results in enhancement of HSC function.Methods:We used in vitro mitochondrial activity and in vivo long‐term blood reconstitution assays as readouts of murine and human HSC functionality.Results:We previously demonstrated that modulation of mitochondrial metabolism in murine and human HSCs, by chemical uncouplers of the electron transport chain or via NAD+ boosting agent Nicotinamide Riboside (NR), results in better long‐term blood production in serially transplanted mice (Vannini N∗, Girotra M∗. et al., Nature Communication 2016 and, Vannini et al., Cell Stem Cell, in press).
Here we proceeded to carry out a screen, using mitochondrial activity as readout, to identify metabolic modulators that enhance HSC function. We found two novel candidates, a natural compound and a vitamin precursor, that modulate mitochondrial activity in both mouse and human HSCs, and resulted in enhanced HSC function post bone‐marrow transplantation. Interestingly, we found that these candidates mediate their effects partially by inducing mitophagy, supporting recent studies highlighting the role of mitophagy as a key driver of HSC function. Moreover, our preliminary analysis reveals that they mediate similar effects in aged human HSCs, making them ideal candidates to revert age‐associated myeloid bias in human patients.Summary/Conclusion:Our data establishes a causal relationship between mitochondrial metabolism and HSC function via mitophagy. It also provides a valuable tool to identify optimal ex vivo conditions for HSC expansion, to improve the outcome for patients suffering from bone marrow insufficiency and to restore the functionality of the immune system in aged individuals.
PF193 NO BLASTS AT DAY 8 IS BETTER THAN MORE IN CORTICO‐SENSITIVE ACUTE LYMPHOBLASTIC LEUKEMIA OF CHILDREN
Authors: Frikha I. , Turki I. , Medhaffer M. , Hdijji S. , Ben Amor I. , Charfi M. , Kassar O. , Ghorbel M. , Kallel F. , Elloumi M. 
Published: June 2019

Background:Acute lymphoblastic leukemia (ALL) had a good prognostic in children and young adults. However, some parameters were predictive of a worse outcome, particularly the poor response to prephase.Aims:We analysed in this study the impact of persistent blasts at day 8 of pre‐phase in cortico‐sensitives patients with ALL.Methods:Between january 2000 and December 2015, we retrospectively analysed the data of 264 children and young adults treated according the EORTC 58951 protocol. Cortico‐sensitivity (Cs) was considered if peripheral blast count is less than 1000/mm3 at day 8 of pre‐phase. The group of cortico‐sensitives ALL was subdivided on two subgroups : the first with no blasts at day 8 (Cs = 0) and the second with more (Csǂ0). We analysed the impact of cortico‐sensitivity on complete remission (CR), relapse and survival (overall survival (OS), event free survival (EFS) and disease free survival (DFS)).Results:One hundred ninety two children and 72 young adults were treated according to the EORTC 58951 protocol. Median age was 10 years (1 to 30 years). Sex ratio was 1,5. B and T phenotype were observed in respectively 64 and 36% of cases. The ALL were stratified in low risk (11 patients), average risk (182 patients), and high risk (71 patients). Two hundred and nine patients (79%) were cortico‐sensitive, among them 150 patients (72%) had no blast at day 8 of pre‐phase. The therapeutics results in our study and into the two groups of cortico‐sensitivity were shown in this table :Summary/Conclusion:Cortico‐sensitivity is one of several parameters that predict good outcome in ALL. Moreover, our study showed that no blasts in peripheral blood smears at day 8of prephase can be considered a good first marker of early blastic clearance and good outcome. In fact, this parameter does not discussed previously in the literature.image
PF199 TARGETING BRD4 IN MLL‐PTD AML
Authors: Pepe F. , McNeil B. , Ozer H.G. , Karunasiri M. , Bill M. , Garzon R. , Dorrance A. 
Published: June 2019

Background:Mixed lineage leukemia (MLL) gene encodes for a nuclear protein that regulates genes involved in development and hematopoiesis. De‐regulation of MLL by chromosome translocations causes acute myeloid leukemia (AML). In addition, about 5–11% of AML patients have MLL‐partial tandem duplication (PTD) mutations. MLL‐PTD is associated with poor prognosis and shorter remission duration in AML. Preliminary studies from our group indicate that when MLL‐PTD is knockdown in AML blasts we observe a mRNA expression profile that is very similar to that of bromodomain 4 (BRD4) inhibition in AML.Aims:We hypothesized that MLL‐PTD interacts with BRD4 forming a complex that regulate key genes in MLL‐PTD positive AML. Therefore treatment with BRD4 inhibitors, such as JQ1, could be a valid therapeutic strategy for MLL‐PTD AML patients.Methods:To identify proteins forming the transcriptional complex regulated by BRD4 and MLL, we conducted a Rapid Immunoprecipitation Mass spectrometry of Endogenous protein (RIME) study using BRD4 and MLL antibodies. Successively, we treated MLL‐PTD positive primary patient cells and CD34+ cord blood from healthy donors as controls with JQ1, and conducted RNA‐seq and ChIP‐seq studies. Finally, we conducted in vitro and in vivo studies on MLL‐PTD harboring samples to assess JQ1 treatment efficiency in inducing cell death in MLL‐PTD AML.Results:RIME analysis using BRD4 and MLL antibodies showed that the 75% of the proteins were in common between the two groups. PANTHER gene ontology analysis identified common pathways between them, suggesting that MLL and BRD4 could be part of similar protein complexes. Next, we treated MLL‐PTD+ primary patient cells, and CD34+ cord blood cells as controls with JQ1 or vehicle, and conducted RNA‐seq and ChIP‐seq studies. RNA expression and peak intensity on promoter region results were combined to identify genes aberrantly expressed in MLL‐PTD+ samples. We identified 98 up‐regulated and 32 down‐regulated genes that were differentially expressed d by JQ1 treatment only in AML samples. The top deregulated genes were validated by RT‐qPCR and ChIP in both human and murine MLL‐PTD+ samples. Next, we treated the MLL‐PTD+ AML cell line (EOL1) and primary murine AML cells from Mll‐PTD/Flt3‐ITD model with JQ1 and showed that JQ1 treatment induced more apoptosis and reduction of living cells in MLL‐PTD+ models respect the relative controls (fold change living cells at 48 hours WT: 0.81, Mll‐PTD+: 0.42, p‐value: 0.0247). Last, treatment of the Mll‐PTD/Flt3‐ITD xenotranplanted BoyJ mice with JQ1 significantly increased their survival compared with controls (median survival CTRL: 75 days, JQ1: 109 days, Mantel‐Cox p‐value: 0.0195). In addition, we observed a reduced engraftment in serial re‐transplantation assays.Summary/Conclusion:Our data support developing a therapeutic strategy to target BRD4 in MLL‐PTD AML.image
PF249 NEXT‐GENERATION‐SEQUENCE‐BASED GENE MUTATION DETECTION AND ITS CLINICAL SIGNIFICANCE IN PATIENTS WITH ACUTE MYELOID LEUKEMIA
Authors: Yu J. , Li T. , Li Y. , Li Y. , Xing H. , Sun H. , Wan D. , Liu Y. , Wang C. , Xie X. , Jiang Z. 
Published: June 2019

Background:Acute myeloid leukemia (AML) is a biologically heterogeneous disease with many different cytogenetic disorders and gene mutations. The advances of the next‐generation sequence (NGS) made it more practical for the clinical research to explore the cytogenetic analysis and mutation testing for the classification and risk assessment of AML patients.Aims:In this study, we sought to investigate the differences in molecular gene mutations between different type of AML patients and the response from those newly diagnosed AML patients to induction chemotherapy.Methods:We retrospectively evaluated 274 hospitalized non‐M3 AML patients. A next‐generation sequencing assay covering 43 genes was used to investigate recurrently mutated genes. The mutational status of fusion genes was determined by real time PCR. Clinical follow up was based on the patients recent clinic visits. Correlation analysis was done based different groups classified by different categories.Results:The median age at diagnosis was 45 year‐old (range 16–81 years). 231 patients were 59 years or younger with the median age of 41 years. Forty‐three patients were 60 years or older with a median age of 69 years (range 60–81 years). At least one non‐synonymous gene mutation was detected in 233 patients (85%). The median number of gene mutations was two (range 0–8). Comparing to the 59 years or younger patients, there was a significantly higher incidence of gene mutation in 60 years and older patients (p <0.01). The mean number of molecular gene mutations at diagnosis was higher in older patients than younger patients (2.92 vs 1.48, p < 0.05). Older patients had significantly higher frequency of ASXL1 (23.3% vs 13.9%, p <0.05), TP53 (11.6% vs 1.7%, p <0.05), IDH2 (18.6% vs 4.3%, p < 0.05) mutations, higher frequency of CEBPA (21.2% vs 14%, p < 0.05). Among 11 patients with no gene mutation received DA (daunorubicin 80 mg/m2 per day on days 1–3 and cytarabine 100 mg/m2 twice per day on days 1–7) as the induction therapy, 8 patients (73%) achieved CR after 1 course induction treatment. Meanwhile, among 21 patients with one or more gene mutations received DA induction therapy, only 12 patients (57%) achieved CR after 1 course induction treatment. More data need to be collected regarding the significance of each individual gene fusion correlating with induction chemotherapy.Summary/Conclusion:A higher incidence of cytogenetic disorders was found in older AML patients with higher burdens of molecular mutations, comparing to younger patients. Also, significantly higher frequency of ASXL1, TP53, IDH2 and CEBPA had been found with older patients. Patients with fewer gene fusion had a better chance of achieving CR when treated with cytarabine and daunorubicin induction chemotherapy.
PF270 IS ACUTE MYELOBLASTIC LEUKEMIA IN CHILDREN UNDER TWO YEARS OF AGE A SPECIFIC ENTITY? A REPORT FROM THE FRENCH ELAM02 STUDY GROUP
Authors: Blais S. , Boutroux H. , Pasquet M. , Leblanc T. , Fenneteau O. , Gandemer V. , Bertrand Y. , Ducassou S. , Michel G. , Nelken B. , Petit A. , Cuccuini W. , Gouache E. , Marceau A. , Baruchel A. , Lapillonne H. , Leverger G. 
Published: June 2019

Background:The clinical and biological characteristics of children under two years (infants) with acute myeloid leukemia (AML) are different from those of older children.Aims:We aimed to describe the specific characteristics of this population and the potential factors that influence the prognosis.Methods:We analyzed data concerning 438 children with newly‐diagnosed AML treated in the ELAM02 protocol between March 2005 and December 2011, of which 103 were under two years old at diagnosis. The evaluation criteria were overall survival (OS) and event‐free survival (EFS) of infants versus older children. The clinical and biological features were secondary criteria.Results:Infants presented more frequent extra‐medullary presentation than older children. They had a significantly higher proportion of skin lesions and central nervous system involvement (15% vs 3%, P < 0.0001 and 26% vs 12%, P = 0.0005, respectively). The global incidence of KMT2A rearrangements was nearly 55% for infants versus 11% for older children (P < 0.0001). Median five‐year OS was 70.4% for infants versus 71.4% for older children (P = 0.83). Five‐year EFS was 67% for infants versus 58% for older children (P = 0.27).Summary/Conclusion:Infants with AML represent a cohort of patients with specific clinical and biological features. These remarkable differences had no significant impact on their outcome in the ELAM02 protocol.