bluuebunny/crossref_metadata_2025_split huggingface dataset:
Depletion of Ras Suppressor-1 (RSU-1) promotes cell invasion of breast cancer cells through a compensatory upregulation of a truncated isoform
Authors: Gkretsi Vasiliki , Kalli Maria , Efstathiades Christodoulos , Papageorgis Panagiotis , Papanikolaou Vassilios , Zacharia Lefteris C. , Tsezou Aspasia , Athanassiou Evangelos , Stylianopoulos Triantafyllos 
Published: July 2019

AbstractExtracellular matrix (ECM)-adhesion proteins and actin cytoskeleton are pivotal in cancer cell invasion. Ras Suppressor-1 (RSU-1), a cell-ECM adhesion protein that interacts with PINCH-1, thus being connected to Integrin Linked Kinase (ILK), alpha-parvin (PARVA), and actin cytoskeleton, is up-regulated in metastatic breast cancer (BC) samples. Apart from the originally-identified gene (RSU-1L), an alternatively-spliced isoform (RSU-1-X1) has been reported. We used non-invasive MCF-7 cells, expressing only RSU-1L, and highly invasive MDA-MB-231-LM2 expressing both isoforms and generated stable shRNA-transduced cells lacking RSU-1L, while the truncated RSU-1-X1 isoform was depleted by siRNA-mediated silencing. RSU-1L depletion in MCF-7 cells resulted in complete abrogation of tumor spheroid invasion in three-dimensional collagen gels, whereas it promoted MDA-MB-231-LM2 invasion, through a compensatory upregulation of RSU-1-X1. When RSU-1-X1 was also eliminated, RSU-1L-depletion-induced migration and invasion were drastically reduced being accompanied by reduced urokinase plasminogen activator expression. Protein expression analysis in 23 human BC samples corroborated our findings showing RSU-1L to be upregulated and RSU-1-X1 downregulated in metastatic samples. We demonstrate for the first time, that both RSU-1 isoforms promote invasion in vitro while RSU-1L elimination induces RSU-1-X1 upregulation to compensate for the loss. Hence, we propose that both isoforms should be blocked to effectively eliminate metastasis.
Efficient White LEDs Using Liquid-state Magic-sized CdSe Quantum Dots
Authors: Sadeghi Sadra , Khabbaz Abkenar Sirous , Ow-Yang Cleva W. , Nizamoglu Sedat 
Published: July 2019

AbstractMagic clusters have attracted significant interest to explore the dynamics of quantum dot (QD) nucleation and growth. At the same time, CdSe magic-sized QDs reveal broadband emission in the visible wavelength region, which advantageously offer simple integration of a single-type of nanomaterial and high color rendering ability for white light-emitting diodes (LEDs). Here, we optimized the quantum yield of magic-sized CdSe QDs up to 22% via controlling the synthesis parameters without any shelling or post-treatment process and integrated them in liquid-state on blue LED to prevent the efficiency drop due to host-material effect. The fabricated white LEDs showed color-rendering index and luminous efficiency up to 89 and 11.7 lm/W, respectively.
Forecasting cell fate during antibiotic exposure using stochastic gene expression
Authors: Rossi Nicholas A. , El Meouche Imane , Dunlop Mary J. 
Published: July 2019

AbstractAntibiotic killing does not occur at a single, precise time for all cells within a population. Variability in time to death can be caused by stochastic expression of genes, resulting in differences in endogenous stress-resistance levels between individual cells in a population. Here we investigate whether single-cell differences in gene expression prior to antibiotic exposure are related to cell survival times after antibiotic exposure for a range of genes of diverse function. We quantified the time to death of single cells under antibiotic exposure in combination with expression of reporters. For some reporters, including genes involved in stress response and cellular processes like metabolism, the time to cell death had a strong relationship with the initial expression level of the genes. Our results highlight the single-cell level non-uniformity of antibiotic killing and also provide examples of key genes where cell-to-cell variation in expression is strongly linked to extended durations of antibiotic survival.
Effects of icariin on long noncoding RNA and mRNA expression profile in the aortas of apoE-deficient mice
Authors: Zhang Yibing , Xu Rui , Li Xiangjun , Tan Qi , Huang Peng , Zhang Yang , Qin Meng , Ren Liqun 
Published: July 2019

Abstract
Objective : The beneficial effects of icariin (ICA) in ameliorating atherosclerosis (AS) are well known, but the underlying protective mechanism has not been fully elucidated. The present study aimed to investigate altered long noncosing RNA (lncRNA) and mRNA expression profiles in ApoE−/− mice after ICA treatment.
Method : The atherosclerotic plaque area was evaluated on high-fat diet (HFD)-induced ApoE−/− mice treated with either ICA or vehicle. LncRNA and mRNA integrated microarrays was performed on aortic tissues. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were utilized to explore the significant function and pathway of the differentially expressed (DE) mRNAs, global signal transduction network were constructed to select key mRNAs, and lncRNA–mRNA co-expression network was built to find out the interactions between lncRNA and mRNA. Quantitative real-time PCR (qPCR) was used to further validate the expressions of selected lncRNAs and mRNAs.
Results : Administration of ICA significantly reduced plaque size after 12 weeks (P&lt;0.05). A total of 1512 DE lncRNAs and 2059 DE mRNAs were identified. The mRNAs: protein kinase C, β (Prkcb), Cyp2c65, Mapk10, Calmodulin 5 (Calm5), Calmodulin-like 3 (Calml3) and Camk4 were selected as hub mRNAs, the correlated lncRNAs in co-expression network were identified as important regulatory lncRNAs. The identified target pairs such as lncRNA-NONMMUT000659/Prkcb may play critical roles in AS development mediated by ICA.
Conclusion : Taken together, our study highlights a panel of DE lncRNAs and mRNAs that could explain the molecular mechanism of ICA’s anti-atherosclerotic effects. The work lays a foundation for subsequent genes functional researches, which could contribute to provide new therapeutic targets for AS.
Visualizing the inner life of microbes: practices of multi-color single-molecule localization microscopy in microbiology
Authors: Vojnovic Ilijana , Winkelmeier Jannik , Endesfelder Ulrike 
Published: July 2019

Abstract
In this review, we discuss multi-color single-molecule imaging and tracking strategies for studying microbial cell biology. We first summarize and compare the methods in a detailed literature review of published studies conducted in bacteria and fungi. We then introduce a guideline on which factors and parameters should be evaluated when designing a new experiment, from fluorophore and labeling choices to imaging routines and data analysis. Finally, we give some insight into some of the recent and promising applications and developments of these techniques and discuss the outlook for this field.
Early experience with cap-assisted endoscopic pancreatic necrosectomy: A technique to enhance safe tissue extraction and decrease interventions
Authors: Puri Nishant , Hallac Alexander , Srikureja Wichit 
Published: July 2019

Abstract
Background and study aim Endoscopic treatment of walled-off pancreatic necrosis (WOPN) has been established as an alternative to operative intervention for well selected patients for many years.
Patients and methods A retrospective database of patients who underwent cap-assisted endoscopic necrosectomy of symptomatic or infected WOPN using the assistance of a sterilized banding cap was constructed. All procedures were performed at a single center between January 2017 and June 2018.
Results Eight patients met the inclusion criteria for this study. Contrast computed tomography scan was obtained between the initial percutaneous or trans-gastric access and initial necrosectomy. The WOPN had a median length of 9.5 cm (range 3.2 – 14) and width of 5.3 cm (range 2.8 – 11.6). Median duration of endoscopic debridement was 69 minutes (range 21 – 105). Four of six patients underwent a second debridement with a median duration of 95 minutes (range 16 – 108). No periprocedural adverse events occurred. Follow-up was at 6 months, and there were no additional endoscopic or percutaneous interventions for recurrent pancreatic fluid collections.
Conclusion The technique of cap-assisted necrosectomy can allow for safe and efficient method of endoscopically treating WOPN.
Tanshinone II A Affects Diabetic Peripheral Neuropathic Pain via Spinal Dorsal Horn Neuronal Circuitry by Modulating Endoplasmic Reticulum Stress Pathways
Authors: Kong Dawei , Guo Zhuangli , Yang Wenqiang , Wang Qi , Yu Yanbing , Zhang Li 
Published: July 2019

AbstractDiabetic peripheral neuropathic pain (DPNP) is a common manifestation of diabetic peripheral neuropathy (DPN). Although the pathogenesis of DPNP remains unclear, the disinhibition of spinal dorsal horn neuronal circuitry mediated by endoplasmic reticulum stress (ERS) is an important mechanism underlying neuropathic pain (NP). Tanshinone II A is mainly used to treat cardiovascular diseases but has also been shown to relieve various types of neuralgia, including DPNP. This study investigated the effects of tanshinone II A in DPNP model rats. We divided animals into two groups: 1) the model (diabetic) group and 2) the tanshinone II A-treatment group. Our results demonstrated that diabetic rats exhibited a decrease in the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL), and that NMT is increased and TWL is prolonged in rats treated with tanshinone II A. Additionally, the levels of ERS-signaling pathway factors in the spinal dorsal horns of rats were lower in the tanshinone II A-treated group than in the diabetic group. Overall, our study demonstrated that the disinhibition of spinal dorsal horn neuronal circuitry mediated by endoplasmic reticulum stress underlies DPNP and is modulated by tanshinone II A treatment.
Is the Energy Expenditure Provided by Exergames Valid?
Authors: Viana Ricardo Borges , Gentil Paulo , Andrade Marília Santos , Vancini Rodrigo Luiz , de Lira Claudio Andre Barbosa 
Published: July 2019

AbstractTo assess energy expenditure (EE) estimated by a metabolic system during the exergame Hollywood Workout on Xbox 360Kinect®
and to compare this measure with the EE estimated by the exergame (EEEx). thirty-four physically active young males performed the Sports Athlete protocol of the Hollywood Workout. Oxygen uptake (V̇O2) and carbon dioxide production (V̇CO2) were measured before and during the protocol using a metabolic system. The absolute means V̇O2 and V̇CO2 were used to calculate the EE during the exergame session (EEMS). The EEEx (kcal) provided by the exergame was collected, and the two measures were compared. Mean V̇CO2 and V̇O2 during the Sports Athlete protocol were 1.8±0.4 L·min−1 (22.9±3.2 mL·kg−1.min−1) and 2.0±0.4 L·m−1 (25.4±2.6 mL·kg−1·min−1), respectively. The EEMS (300.3±53.4 kcal) was 88.6±34.7% greater than the EEEx (160.3±16.5 kcal; p<0.0001; d=3.7 [large effect size]). The EEMS was almost double EEEx. Thus, it is necessary to use caution when using EE information provided by an exergame interface. Further, future studies need to evaluate EE provided by other exergames. The companies that produce the games must be more attentive and adopt more precise methods in order to provide more accurate information to the users.
Bilateral Striatal Necrosis with Polyneuropathy with a Novel SLC25A19 (Mitochondrial Thiamine Pyrophosphate Carrier OMIMI*606521) Mutation: Treatable Thiamine Metabolic Disorder—A Report of Two Indian Cases
Authors: Gowda Vykuntaraju K. , Srinivasan Varunvenkat M. , Jehta Kapil , Bhat Maya D. 
Published: July 2019

Abstract
Background SLC25A19 gene mutations cause Amish congenital lethal microcephaly and bilateral striatal necrosis with polyneuropathy. We are reporting two cases of bilateral striatal necrosis with polyneuropathy due to SLC25A19 gene mutations.
Methods A 36-month-old boy and a 5-year-old girl, unrelated, presented with recurrent episodes of flaccid paralysis and encephalopathy following nonspecific febrile illness. Examination showed dystonia and absent deep tendon reflexes.
Results Nerve conduction studies showed an axonal polyneuropathy. Magnetic resonance imaging (MRI) of the brain in both cases showed signal changes in the basal ganglia. Next-generation sequencing revealed a novel homozygous missense variation c.910G>A (p.Glu304Lys) in the SLC25A19 gene in the boy and a homozygous mutation c.869T > A (p. Leu290Gln) in the SLC25A19 gene in the girl. Mutations were validated by Sanger sequencing, and carrier statuses of parents of both children were confirmed. Both children improved with thiamine supplementation.
Conclusion If any child presents with recurrent encephalopathy with flaccid paralysis, dystonia, and neuropathy, a diagnosis of bilateral striatal necrosis with polyneuropathy due to SLC25A19 mutations should be considered and thiamine should be initiated.
Congenital Segmental Intestinal Dilatation: A 25-Year Review with Long-Term Follow-up at the Medical University of Innsbruck, Austria
Authors: Sergi Consolato , Hager Thomas , Hager Josef 
Published: July 2019

Background and Aim Congenital segmental intestinal dilatation (CSID) is a neonatal condition with unclear etiology and pathogenesis. Typically, the newborn with CSID presents with a limited (circumscribed) bowel dilatation, an abrupt transition between normal and dilated segments, neither intrinsic nor extrinsic perilesional obstruction, and no aganglionosis or neuronal intestinal dysplasia. We aimed to review this disease and the long-term follow-up at the Children's Hospital of the Medical University of Innsbruck, Tyrol, Austria.
Study Design Retrospective 25-year review of medical charts, electronic files, and histopathology of neonates with CSID.
Results We identified four infants (three girls and one boy) with CSID. The affected areas included duodenum, ileum, ascending colon, and sigmoid colon. Noteworthy, all patients presented with a cardiovascular defect, of which two required multiple cardiac surgical interventions. Three out of the four patients recovered completely. To date, the three infants are alive.
Conclusion This is the first report of patients with CSID and cardiovascular defects. The clinical and surgical intervention for CSID also requires a thorough cardiologic evaluation in these patients. CSID remains an enigmatic entity pointing to the need for joint forces in identifying common loci for genetic investigations.
“Yes, he is, at least, entertaining”
Authors: Malthus Miriam 
Published: July 2019

AbstractThis paper investigates facework and identity construction on a pro-community water fluoridation Facebook page, drawing on rapport management (Spencer-Oatey 2000) and Culpeper’s (1996,2010,2011) taxonomy of impoliteness in English. In contrast to previous work on conflictual political talk on social media, which focuses largely on right/left or socially conservative/progressive polarised topics, it addresses discourse on a topic where conflict is between factions aligned with or against the scientific establishment. The paper shows members of an activist group engaging in face-aggravating behaviour against an ideologically opposed commenter. Even when they profess to be educating the commenter, the core goals of their behaviour are enhancing their own quality and identity face within the group by antagonising the outsider; participants construct an expert identity through performing superior intelligence and education, expressed through displays of scientific knowledge and creative forms of linguistic impoliteness.
Changes in Voluntary Admission and Restraint use after a Comprehensive Tobacco-Free Policy in Inpatient Psychiatric Health Facilities
Authors: Scheeres Annaka , Xhezo Regina , Julius Rose , Coffman Ryan , Frisby Jarma , Weber Luise , Streeter Jessica , Leone Frank , Bettigole Cheryl , Lawman Hannah 
Published: July 2019

Background: Cardiovascular and respiratory diseases, predominantly due to tobacco use, are the leading causes of death among individuals with serious and persistent mental illness. However, many psychiatric health facilities do not routinely treat tobacco use disorder. The purpose of the current study was to examine the impact of implementing a tobacco-free policy in inpatient psychiatric health facilities in a large, urban setting on behavioral problems, treatment access, and tobacco treatment. Methods: Data on seclusion and restraint incidents, voluntary commitment at admission for each hospitalization episode, and nicotine replacement therapy (NRT) prescriptions were collected through secondary analysis of Medicaid administrative records from baseline in January 2015 ( n = 8983) to follow-up in December 2016 ( n = 9685) at 14 inpatient psychiatric health facilities. Results: There were no significant changes from baseline to follow-up in odds of seclusion and restraint incidents or voluntary admission status. There was a significant increase in the odds of NRT prescriptions at both 30 and 180 days post discharge (odds ratio [OR] range = 1.58–2.09, P < .01). Conclusions: In a large, urban setting among Medicaid enrollees, implementation of a tobacco-free policy in inpatient psychiatric health facilities had no negative impact on behavioral problems or treatment access and improved access to NRT, although overall NRT use remained low. This study challenges perceptions among some providers that addressing tobacco use disorder will negatively impact treatment outcomes in individuals with serious mental illness. These findings support tobacco-free policies in psychiatric health facilities and the role of psychiatric health providers in treating tobacco use in this population, which is at high risk for tobacco-related mortality.
Opioid, Cocaine, and Amphetamine use Disorders are Associated with Higher30-Day Inpatient Readmission Rates in the United States
Authors: Mejia de Grubb Maria C. , Salemi Jason L. , Gonzalez Sandra J. , Chima Charles C. , Kowalchuk Alicia A. , Zoorob Roger J. 
Published: July 2019

Background: Patients with substance use disorders (SUDs) are more likely to experience serious health problems, high healthcare utilization, and premature death. However, little is known about the contribution of SUDs to medical 30-day readmission risk. We examined the association between SUDs and 30-day all cause readmission among non-pregnant adult in-patients in the US. Methods: We conducted a retrospective study using 2010–2014 data from the Nationwide Readmissions Database. Our primary focus was on opioid use compared to stimulant use (cocaine and amphetamine) identified by ICD-9-CM diagnosis codes in index hospitalizations. Multivariable logistic regression models were used to estimate adjusted odds ratios and 95% CI representing the association between substance use and 30-day readmission, overall and stratified by the principal reason for the index hospitalization. Results: Nearly 118 million index hospitalizations were included in the study, 4% were associated with opioid or stimulant use disorder. Readmission rates for users (19.5%) were higher than for nonusers (15.7%), with slight variation by the type of substance used: cocaine (21.8%), opioid (19.0%), and amphetamine (17.5%). After adjusting for key demographic, socioeconomic, clinical, and health system characteristics, SUDs and stimulant use disorders increased the odds of 30-day all-cause readmission by 20%. Conclusions: Reducing the frequency of inpatient readmission is an important goal for improving the quality of care and ensuring proper transition to residential/outpatient care among patients with SUDs. Differences between groups may suggest directions for further investigation into the distinct needs and challenges of hospitalized opioid- and other drug-exposed patients.
Development of a regulatable low-temperature protein expression system using the psychrotrophic bacterium, Shewanella livingstonensis Ac10, as the host
Authors: Kawai Soichiro , Kawamoto Jun , Ogawa Takuya , Kurihara Tatsuo 
Published: July 2019

ABSTRACT
A low-temperature protein expression system is useful for the production of thermolabile proteins. We previously developed a system that enables constitutive protein production at low temperatures, using the psychrotrophic bacterium Shewanella livingstonensis Ac10 as the host. To increase the utility of this system, in the present study, we introduced a repressible promoter of the trp operon of this bacterium into the system. When ß-lactamase was produced under the control of this promoter at 18°C and 4°C, the yields were 75 and 33 mg/L-culture, respectively, in the absence of l-Trp, and the yields were decreased by 72% and 77%, respectively, in the presence of l-Trp. We also found that 3-indoleacrylic acid, a competitive inhibitor of the Escherichia coli trp repressor, increased the expression of the reporter gene. This repressible gene expression system would be useful for regulatable recombinant protein production at low temperatures.
Effects of rising atmospheric CO2, climate change, and nitrogen deposition on aboveground net primary production in a temperate forest
Authors: Wang Wen J , Ma Shuang , He Hong S , Liu Zhihua , Thompson Frank R , Jin Wenchi , Wu Zheng Fang , Spetich Martin A , Wang Lei , Xue Song , Zhang Wenguang , Wang Xianwei 
Published: July 2019

AbstractTemperate forests regrowing from historical land use and land cover change in the eastern US serve as carbon (C) sinks. Environmental drivers have been significantly altered (e.g. rising atmospheric CO2concentration, warmer temperature, and elevated nitrogen (N) deposition) and will have a wide range of impacts on future forest C sinks. However, the interactions among these environmental drivers are unclear and their effects are subject to uncertainty. We assessed the combined and interactive effects of rising CO2concentration, climate change (temperature, precipitation), and N deposition on forest aboveground net primary production (ANPP) and their relative contribution to ANPP changes of a temperate forest in the eastern US. We used a process-based ecosystem model PnET-day to simulate coupled cycles of C, water, and N of forest ecosystems. We found that (1) climate change exerted negative effects on ANPP (−0.250 kg C m−2yr−1) whereas rising CO2and N deposition enhanced ANPP (+0.253, +0.014 kg C m−2yr−1); (2) climate change interacted with rising CO2and N deposition to decrease ANPP (−0.032, −0.018 kg C m−2yr−1); rising CO2and N deposition acted in synergy to increase ANPP (+0.014 kg C m−2yr−1); (3) changes in ANPP were mainly attributed to rising CO2and climate change whereas N deposition effects and any two- or three-factor interactive effects were relatively small. Our results suggest that the total negative effect sizes will not be offset by the total positive effect sizes, thus resulting in reductions in forest ANPP over the 21st century.
Pilot-scale AC electric arc furnace plasma characterization
Authors: Pauna Henri , Willms Thomas , Aula Matti , Echterhof Thomas , Huttula Marko , Fabritius Timo 
Published: July 2019

Abstract
Fundamental knowledge of the electric arc properties is important for the development of process control of electric arc furnaces. In this work, a pilot-scale AC electric arc has been studied with optical emission spectroscopy together with filtered camera footage. The properties of the arcs were determined with plasma diagnostics and image analysis in order to obtain both the characteristic plasma parameters and the physical form of the arc. The plasma temperatures, ranging from 4500 to 9000 K, were derived individually for three elements. The electron densities of the plasma were between 1018 and 1020 cm−3 and fulfilled the local thermal equilibrium criterion, but the plasma temperatures derived from atomic emission lines for different elements had high and unpredictable differences. The properties of the electric arcs have been studied with respect to the arc length derived from the image analysis. The slag composition, especially the relative FeO content of over 30%, was observed to have a notable effect on the brightness of the arc on slag and thus also on the radiative heat transfer.