bluuebunny/crossref_metadata_2025_split huggingface dataset:
Konstruksi Kecantikan dalam Novel Midah Si Manis Bergigi Emas dan Cantik Itu Luka
Authors: Farida Maesaroh  
Published: October 2024

Cantik Itu Luka dan Midah Si Manis Bergigi Emas dan Cantik Itu Luka merupakan novel yang mengambil perempuan sebagai tokoh utamanya. Novel Midah Si Manis Bergigi Emas karya Pramoedya Ananta Toer dan Novel Cantik Luka karya Eka Kurniawan merupakan dua karya besar yang mendapatkan berbagai penghargaan. Bertema pada perjuangan perempuan, kedua novel ini memaknai cantik yang dikonstruksikan melalui tokoh-tokoh perempuan. Penelitian ini bertujuan untuk mendeskripsikan konstruksi kecantikan dalam novel Midah Si Manis Bergigi Emas dan novel Cantik Itu Luka dengan pendekatan deskriptif kualitatif. Metode yang digunakan dalam penelitian ini adalah sastra bandingan, khususnya untuk mendapatkan persamaan atau perbedaan dalam kedua novel tersebut. Teori konstruksi kecantikan dengan pendekatan psikologi sastra Sigmund Freud digunakan untuk memperoleh pemaknaan kecantikan dari kepribadian tokoh perempuan. Hasil analisis dari kedua novel mengkonstruksikan kecantikan bukan hanya soal cantik dari dalam ataupun luar, juga sebagai pandangan bahwa cantik itu bisa membawa kemalangan dan malapetaka. Perbandingan tentang kecantikan yang muncul dari novel Midah Si Manis Bergigi Emas memfokuskan kecantikan pada satu kepribadian tokoh yang menjadi pusat dari alur ceritanya, yaitu Midah. Novel Cantik Itu Luka direkonstruksi oleh beberapa tokoh perempuan seperti Dewi Ayu, Cantik, Alamanda, dan Aneu dengan beragam stigma yang didapatkannya.
The economic and social burden of congenital thrombotic thrombocytopenic purpura (cTTP) in Italy
Authors: Rumi Filippo , Basile Michele , Di Brino Eugenio , Fortunato Agostino , Antonini Debora 
Published: October 2024

Background: Congenital thrombotic thrombocytopenic purpura (cTTP) is a rare genetic disorder characterized by an abnormal clotting process leading to serious health complications such as thrombocytopenia and hemolytic anemia. Despite its clinical significance, the estimated broader economic and social impacts in Italy on patients and society remain underexplored.
Methods: A detailed questionnaire, developed and validated by a panel of cTTP experts, was distributed to healthcare professionals and patient associations to gather comprehensive data on the economic and social burden of cTTP in Italy. The study was focused on direct and indirect costs associated with the disease management of 15 patients with cTTP, as well as out-of-pocket expenses incurred by patients.
Results: The analysis revealed that of the 15 patients diagnosed with cTTP in Italy, 60% were adults aged 40-55 years. Productivity loss and diminished quality of life dominated the economic burden of cTTP, accounting for 81.05% of total costs. Direct medical costs, while substantial, represented a smaller portion of the overall financial impact. Additionally, out-of-pocket expenses, though constituting the smallest fraction, underscored the financial contributions made by patients towards their own care management.
Conclusion: The findings underscore the need for a multidisciplinary and holistic approach to cTTP management that extends beyond the clinical treatment. Addressing the economic and social implications of the disease is crucial for improving patient well-being and ensuring the sustainability of healthcare systems. The study advocates for increased awareness, research, and access to care for cTTP patients, highlighting the importance of comprehensive support networks to mitigate the disease’s broader societal impacts, estimated to be around 2 million euro for 15 patients in 10 years.
Subunit-specific mechanisms of isoflurane-induced acute tonic inhibition in dentate gyrus granule neuron
Authors: Yu Zhiqiang , Chen Xiaodan , Liu Zheng , Ding Ran , Xu Jin 
Published: October 2024

Prolonged exposure to volatile anesthetics may raise the risk of developing cognitive impairment by acting on gamma-a Aminobutyric acid A receptors (GABAAR). The dentate gyrus plays an important role in the hippocampus and has a high potential for neural plasticity. However, it is unknown whether prolonged anesthesia induces a change in acute phasic or tonic inhibition in dentate gyrus granule cells (DGGCs) by acting on GABAAR. In order to verify the effects of volatile anesthetics on the current in DGGCs, a whole-cell patch was applied to record acute brain slices, and this study indicated that 4 h but not 2 h of isoflurane (ISO) exposure induced significantly larger tonic currents in DGGCs other than hippocampal CA1 pyramidal and thalamic relay neurons. Furthermore, this study demonstrated that the increased tonic current in DGGCs was dependent on the δ subunit-containing GABAARs by using transgenic δ subunit knockout mice. In conclusion, the δ subunit specific GABAAR is the key element that increased acute tonic inhibition in DGGCs of mice after prolonged ISO exposure, which may be one of the mechanisms of ISO neurotoxicity to the developing brain.
Imaging human coronary cholesterol/urate crystals with cross-polarized micro-optical coherence tomography
Authors: Nishimiya Kensuke , Sharma Gargi , Singh Kanwarpal , Ahsen Osman O. , Gardecki Joseph A. , Tearney Guillermo J. 
Published: October 2024

IntroductionBirefringent crystals such as monosodium-urate (MSU) and cholesterol crystals (CC) likely contribute to the progression of coronary artery disease (CAD) due to their potential to exacerbate inflammation through inflammatory cytokine activation. Here, we present cross-polarized micro-optical coherence tomography (CP-µOCT) for visualizing individual birefringent crystals in human coronary arteries.Methods and resultsHuman cadaver coronary arteries with a history of CAD with or without gout were dissected for CP-µOCT imaging. Specimens were processed for histological identification of birefringence under polarization light microscopy (PLM). CP-µOCT visualized needle-crystals that appeared as long projections in orthogonal planes, and PLM confirmed that CP-µOCT-delineated needle-crystals demonstrated negative birefringence. The needle-crystals were dissolved after immersion in uricase (p &lt; 0.05), and thus were MSU. CP-µOCT was three-dimensionally volume-rendered for counting MSU and CCs in 79 regions of interest sized [750 (x) × 500 (y) × 400 (z) µm]. Crystal counts were normalized by the total coronary length utilized. The relationship between CP-µOCT-delineated MSU counts and those seen in corresponding histology, and the difference in coronary MSU amongst gout vs. non-gout patients was analyzed. CP-µOCT-delineated MSU counts were significantly correlated with MSU counted by PLM-based histology (R = 0.98, p &lt; 0.01), and with histology-derived intimal thickening (R = 0.51, p &lt; 0.01). MSU and CCs were both significantly greater in gout patients compared with non-gout patients (p &lt; 0.05).DiscussionThese results demonstrate a significant increase in CP-µOCT-delineated crystals in gout vs. non-gout patients, suggesting that this technology can be used to improve our understanding of crystal-driven coronary pathogenesis.
Screening of m6A-associated ferroptosis-related genes in atherosclerosis based on WGCNA
Authors: Jiang Meiling , Zhao Weidong , Wu Liyong , Zhu Guofu 
Published: October 2024

BackgroundN6-methyladenosine (m6A) has been shown to mediate ferroptosis but its role in atherosclerosis (AS) is unclear.MethodsDifferentially expressed m6A-associated ferroptosis-related genes (DE-m6A-Ferr-RGs) were obtained using differential expression analysis and Pearson correlation analysis. Weighted gene co-expression network analysis (WGCNA) was also performed. The intersection of the module genes and the DE-m6A-Ferr-RGs were recorded as candidate m6A-Ferr-related signature genes. Finally, the m6A-Ferr-related signature genes were screened using least absolute shrinkage and selection operator (LASSO) analysis. Expression validation, receiver operating characteristic ( mapping, and immune correlation analysis were also performed based on the m6A-Ferr-related signature genes. The expression of m6A-Ferr-related signature genes was further validated using a real-time polymerase chain reaction (RT-qPCR).ResultsIn total, 6,167 differentially expressed genes were intersected with 24 m6A- and 259 ferroptosis-related genes, respectively, resulting in 113 DE-m6A-Ferr-RGs obtained using Pearson’s correlation analysis. The module genes obtained from the WGCNA and the 113 DE-m6A-Ferr-RGs were intersected to obtain 48 candidate m6A-Ferr-related signature genes. LASSO analysis was performed and six m6A-Ferr-related signature genes were screened. In addition, the area under the curve values of all six m6A-Ferr-related signature genes were greater than 0.7, indicating that they had potential diagnostic value. Furthermore, the RT-qPCR results revealed that the expression of SLC3A2, NOX4, and CDO1 was consistent with the transcriptome level. Moreover, there was a significant difference in two types of immune cells between the AS and control groups. Naive B cells, CD8+ T cells, regulatory T cells, and activated natural killer cells were positively correlated with CDO1 and NOX4 but negatively correlated with ATG7, CYBB, and SLC3A2.ConclusionIn total, three m6A-Ferr-related signature genes (NOX4, CDO1, and SLC3A2) were obtained through a series of bioinformatics analyses and an RT-qPCR.
Technoference in infant feeding: the impact of maternal digital media use during breastfeeding on maternal attention and mother-infant interactions
Authors: Mason Emma M. , Riccabona Tyne M. , Ventura Alison K. 
Published: October 2024

IntroductionParents' sensitivity and responsiveness to their infants may be affected by the widespread availability and use of mobile devices. The present study examined the impact of maternal digital media use on maternal attention and the quality of mother-infant interaction during breastfeeding.MethodsMothers and infants (n = 25 dyads) participated in a within-subject experiment. Mothers breastfed their infants under one experimental and one control condition, counterbalanced across two laboratory visits. During the Digital Media condition, mothers watched a television show on a tablet. During the Control condition, mothers listened to classical music at ambient levels. Video records were later coded to assess maternal attention to the infant, tablet, or elsewhere and evaluate the quality of mother-infant interaction.ResultsThere were more disruptions in maternal attention to the infant during the Digital Media (M = 3.7, S.E. = 0.2 per minute) vs. Control condition (M = 1.7, S.E. = 0.2 per minute, p &lt; 0.001). The proportion of the meal duration mothers spent focused on their infant was significantly lower during the Digital Media (M = 52.5%, S.E. = 3.9) vs. Control condition (M = 83.9%, S.E. = 4.0%, p &lt; 0.001). Lower maternal attention to the infant was associated with lower maternal sensitivity to cues (p = 0.03) and cognitive growth fostering (p = 0.002), as well as lower infant clarity of cues (p = 0.001). Lower maternal attention was also associated with less socioemotional growth fostering (p &lt; 0.001) and lower infant responsiveness to the mother (p &lt; 0.001) regardless of whether digital media was present or absent, but during the Digital Media condition, mothers engaged in more socioemotional growth fostering (p = 0.004) and infants were more responsive to mothers (p = 0.03).DiscussionThe presence of digital media during infant feeding led to more interruptions to mothers' attention to their infants and the time mothers spent focused on digital media displaced time spent focused on their infants. The degree to which mothers were attentive to their infants vs. digital media was a more important predictor of most aspects of interaction quality than the mere presence of digital media.
Successful eculizumab treatment as an adjunctive therapy to desensitization in ABO-incompatible living donor kidney transplantation and its molecular phenotypes
Authors: Heo Ga Young , Jung Minsun , Piao Honglin , Kim Hyun Jeong , Kim Hyung Woo , Lee Juhan , Huh Kyu Ha , Kim Beom Seok , Yang Jaeseok 
Published: October 2024

IntroductionABO-incompatible (ABOi) kidney transplantation (KT) has become an important option to overcome organ shortage. Plasmapheresis/rituximab-based desensitization therapy has successfully reduced anti-ABO antibody levels and suppressed antibody-mediated rejection (AMR) in ABOi KT. However, high titers of anti-ABO antibodies in some patients are refractory to standard desensitization, leading to loss of KT opportunities or AMR.MethodsEculizumab treatment was used an adjunctive therapy to rescue high-titer ABOi KT patients refractory to plasmapheresis/rituximab-based desensitization. Molecular phenotypes of allograft biopsies and cellular phenotypes of peripheral blood mononuclear cells of eculizumab group were compared with those of control groups using the Banff Human Organ Transplant gene panel and flow-cytometric analysis, respectively.ResultsThe initial titers of anti-ABO antibodies in the two patients were 1:512 and &gt;1:1024; the final pre-transplant titers after desensitization were 1:128 and 1:64. Both patients received eculizumab from KT day to two or four weeks post-KT and maintained stable renal function up to one-year post-transplantation without overt infection, despite early episodes of probable AMR or borderline T cell-mediated rejection. Molecular phenotype analysis revealed that gene expression patterns in the ABOi KT with eculizumab group overlapped with those in the ABOi KT with AMR group more than in the ABOi KT without AMR group, except for complement pathway-related gene expression. Anti-ABO antibody titers decreased to low levels 1–3 months post-transplant in the eculizumab group in parallel with decreasing anti-B-specific B cells.ConclusionsShort-term eculizumab therapy is promising for rescuing ABOi KT recipients with high anti-ABO antibody titers refractory to plasmapheresis-based desensitization therapy.
Cuproptosis-related lncRNAs emerge as a novel signature for predicting prognosis in prostate carcinoma and functional experimental validation
Authors: Lu Yangbai- , Wu Jinfeng- , Li Xianzhe , Leng Qu- , Tan Jian- , Huang Hongxing- , Zhong Rui- , Chen Zhenjie- , Zhang Yongxin- 
Published: October 2024

BackgroundProstate cancer (PCa) is one of the most common malignancies of the urinary system. Cuproptosis, a newly discovered form of cell death. The relationship between cuproptosis-related long non-coding RNAs (ClncRNAs) related to PCa and prognosis remains unclear. This study aimed to explore the clinical significance of novel ClncRNAs in the prognostic assessment of PCa.MethodsClncRNAs and differentially expressed mRNAs linked to these ClncRNAs were identified using Pearson’s correlation and differential expression analyses. A prognostic signature (risk score) comprising three ClncRNAs was established based on multivariable Cox regression analysis. The predictive performance of this ClncRNAs signature was validated using receiver operating characteristic curves and nomograms. Finally, further in vitro cell experiments were conducted for validation, including quantitative polymerase chain reaction (qPCR), western blot (WB), cell proliferation assays, cell migration assays, cell invasion assays, apoptosis, and cell cycle analysis.ResultsWe constructed a prognostic signature of ClncRNAs for PCa comprising three key differentially expressed ClncRNAs(AC010896-1, AC016394-2, and SNHG9). Multivariable Cox regression analysis indicated that clinical staging and risk scores of the ClncRNAs signature were independent prognostic factors for PCa. Compared to other clinical features, the ClncRNAs signature exhibited higher diagnostic efficiency and performed well in predicting the 1-, 3-, and 5-year progression-free intervals (PFIs) for PCa. Notably, in terms of immune activity, PCa patients with high-risk scores exhibited higher tumor mutational burden (TMB) levels, while their Tumor Immune Dysfunction and Exclusion (TIDE) scores were lower than those of PCa patients with low-risk scores. Additionally, in vitro cellular functional experiments, we knocked down SNHG9 that is the most significantly differentially expressed ClncRNA among the three key ClncRNAs. SNHG9 knockdown resulted in a significant increase in G1 phase cells and a decrease in S and G2 phases, indicating inhibition of DNA synthesis and cell cycle progression. Colony formation assays showed reduced clonogenic ability, with fewer and smaller colonies. Western blot analysis revealed the upregulation of the key cuproptosis-related mRNAs FDX1 and DLST. These findings suggested that SNHG9 promotes PCa cell proliferation, migration, and invasion.ConclusionBuilding on the three ClncRNAs, we identified a novel prognostic signature of PCa. The ClncRNA SNHG9 can promote PCa cell proliferation, migration, and invasion.
Genetically predicted plasma metabolites mediate the causal relationship between gut microbiota and primary immune thrombocytopenia (ITP)
Authors: Hong Yang , Zhang Cuilin , Shen Kai , Dong Xiaoqing , Chen Bing 
Published: October 2024

BackgroundPrimary immune thrombocytopenia (ITP) is an immune-mediated hematologic disorder characterized by a reduction in platelet count, increasing the risk of bleeding. Recent studies have indicated a close association between alterations in gut microbiota and the development of ITP. However, the mechanisms by which gut microbiota influence the occurrence and progression of ITP through plasma metabolites remain poorly understood. Evidence suggests extensive interactions between gut microbiota and plasma metabolites, implying a potential role for gut microbiota in influencing ITP through alterations in plasma metabolites, which requires further investigation.MethodsIn this study, summarized GWAS data (including 211 gut microbiota taxa, 1,400 plasma metabolites or ratios, and an ITP patient cohort) were retrieved from the MiBioGen and GWAS Catalog databases. Using a two-sample Mendelian randomization (MR) approach, we screened gut microbiota and plasma metabolites potentially causally related to ITP. We further identified plasma metabolites serving as mediators through which gut microbiota affect ITP and calculated the strength of the mediation effect. To ensure result stability, we primarily used the inverse variance weighted (IVW) method as the main judgment index. We also utilized MR Egger and inverse variance weighted methods to detect heterogeneity in the results, and employed MR-Egger and MR-PRESSO methods to assess the presence of pleiotropy.ResultsThough two-sample MR analysis, 8 gut microbiota taxa were found to have causal relationships with ITP. After excluding six plasma metabolites with pleiotropy, 39 plasma metabolites were found to be causally related to ITP (P &lt; 0.05). Eleven plasma metabolites were identified as having causal relationships between gut microbiota and plasma metabolites. Finally, using the delta method, it was calculated that Sphingomyelin levels (8.0%, 95%CI: 0.9% to 11.5%, P = 0.047) and Glucose-to-mannose ratio (6.5%, 95%CI: 0.7% to 9.5%, P = 0.039) are intermediates for Intestinimonas influencing ITP, while Bilirubin (Z,Z) to etiocholanolone glucuronide ratio (5.6%, 95%CI: 4.7% to 6.9%, P = 0.043) is an intermediate for Senegalimassilia influencing ITP.ConclusionGut microbiota can influence the development of ITP through changes in plasma metabolites. Sphingomyelin levels, Glucose-to-mannose ratio, and Bilirubin (Z,Z) to etiocholanolone glucuronide ratio are newly discovered intermediates through which gut microbiota influence ITP, providing potential indicators and targets for clinical diagnosis and treatment. This study highlights the intricate relationship between gut microbiota and plasma metabolites in the context of ITP, suggesting new avenues for clinical diagnosis and treatment.
Effects of plastic film mulching on soil microbial carbon metabolic activity and functional diversity at different maize growth stages in cool, semi-arid regions
Authors: Kong Meng , Huang Ming-Jing , Zhang Zhi-Xian , Long Jiang , Siddique Kadambot H. M. , Zhang Dong-Mei 
Published: October 2024

IntroductionPlastic film mulching has been widely used to enhance soil hydrothermal conditions and increase crop yields in cool, semi-arid areas. However, its impact on soil microbial carbon metabolic activity and functional diversity during plant growth remains unclear despite their important roles in nutrient cycling and soil quality evaluation.MethodsThis study used the Biolog EcoPlate technique to investigate the dynamics and driving factors of soil microbial carbon metabolic activity and functional diversity at different maize growth stages following plastic film mulching.Results and discussionThe results revealed that film mulching significantly increased microbial carbon metabolic activities [represented by average well color development (AWCD)] by 300% at the seedling stage and by 26.8% at maturity but decreased it by 47.4% at the flowering stage compared to the control (without mulching). A similar trend was observed for the microbial functional diversity index. Redundancy analysis identified soil moisture (SM), soil temperature (ST), dissolved organic carbon (DOC), microbial biomass carbon (MBC), and bacteria amounts as the primary factors influencing changes in soil microbial carbon source utilization. The mulch treatment significantly increased SM at all growth stages, while its warming effect disappeared at the flowering stage. Soil DOC, MBC, and bacterial populations were notably higher under mulching at the seedling and maturity stages but lower at the flowering stage. Pearson correlation analysis showed that changes in SM, ST, DOC, MBC, and bacterial populations positively correlated with the utilization of all carbon source classes, AWCD, and functional diversity indexes after film mulching. Furthermore, maize grain yield and water use efficiency increased by 142 and 129%, respectively, following film mulching. In conclusion, plastic film mulching enhanced soil microbial carbon metabolic activity and functional diversity at the seedling and maturity stages, improving crop yields in cool, semi-arid areas. Furthermore, the decrease in soil carbon metabolic capacity at flowering stage highlights that supplementing soil carbon sources should be considered after continuous film mulching to sustain or enhance farmland productivity and soil quality.