bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cold spot microrefugia hold the key to survival for Brazil's Critically Endangered Araucaria tree
Authors: Wilson Oliver J. , Walters Richard J. , Mayle Francis E. , Lingner Débora V. , Vibrans Alexander C. 
Published: July 2019

AbstractBrazil's Araucaria tree (Araucaria angustifolia) is an iconic living fossil and a defining element of the Atlantic Forest global biodiversity hotspot. But despite more than two millennia as a cultural icon in southern Brazil, Araucaria is on the brink of extinction, having lost 97% of its extent to 20th‐century logging. Although logging is now illegal, 21st‐century climate change constitutes a new—but so far unevaluated—threat to Araucaria's future survival. We use a robust ensemble modelling approach, using recently developed climate data, high‐resolution topography and fine‐scale vegetation maps, to predict the species' response to climate change and its implications for conservation on meso‐ and microclimate scales. We show that climate‐only models predict the total disappearance of Araucaria's most suitable habitat by 2070, but incorporating topographic effects allows potential highland microrefugia to be identified. The legacy of 20th‐century destruction is evident—more than a third of these likely holdouts have already lost their natural vegetation—and 21st‐century climate change will leave just 3.5% of remnant forest and 28.4% of highland grasslands suitable for Araucaria. Existing protected areas cover only 2.5% of the surviving microrefugia for this culturally important species, and none occur in any designated indigenous territory. Our results suggest that anthropogenic climate change is likely to commit Araucaria to a second consecutive century of significant losses, but targeted interventions could help ensure its survival in the wild.
Deep soil inventories reveal that impacts of cover crops and compost on soil carbon sequestration differ in surface and subsurface soils
Authors: Tautges Nicole E. , Chiartas Jessica L. , Gaudin Amélie C. M. , O'Geen Anthony T. , Herrera Israel , Scow Kate M. 
Published: July 2019

AbstractIncreasing soil organic carbon (SOC) via organic inputs is a key strategy for increasing long‐term soil C storage and improving the climate change mitigation and adaptation potential of agricultural systems. A long‐term trial in California's Mediterranean climate revealed impacts of management on SOC in maize‐tomato and wheat–fallow cropping systems. SOC was measured at the initiation of the experiment and at year 19, at five depth increments down to 2 m, taking into account changes in bulk density. Across the entire 2 m profile, SOC in the wheat–fallow systems did not change with the addition of N fertilizer, winter cover crops (WCC), or irrigation alone and decreased by 5.6% with no inputs. There was some evidence of soil C gains at depth with both N fertilizer and irrigation, though high variation precluded detection of significant changes. In maize‒tomato rotations, SOC increased by 12.6% (21.8 Mg C/ha) with both WCC and composted poultry manure inputs, across the 2 m profile. The addition of WCC to a conventionally managed system increased SOC stocks by 3.5% (1.44 Mg C/ha) in the 0–30 cm layer, but decreased by 10.8% (14.86 Mg C/ha) in the 30–200 cm layer, resulting in overall losses of 13.4 Mg C/ha. If we only measured soil C in the top 30 cm, we would have assumed an increase in total soil C increased with WCC alone, whereas in reality significant losses in SOC occurred when considering the 2 m soil profile. Ignoring the subsoil carbon dynamics in deeper layers of soil fails to recognize potential opportunities for soil C sequestration, and may lead to false conclusions about the impact of management practices on C sequestration.
A review and meta‐analysis of intraspecific differences in phenotypic plasticity: Implications to forecast plant responses to climate change
Authors: Matesanz Silvia , Ramírez‐Valiente José Alberto 
Published: July 2019

AbstractAimMany studies use differences among plant populations to infer future plant responses, but these predictions will provide meaningful insights only if patterns of plasticity among populations are similar (i.e., in the absence of population‐by‐environment interaction, P × E). In this study, we tested whether P × E is considered in climate change studies. Specifically, we evaluated whether population differentiation varies across environments and whether P × E is determined by aspects of the study system and experimental design.LocationGlobal.MethodsWe conducted a literature search in the Thomson Reuters Web of Science database to identify studies assessing population differentiation in a climate change context. We quantified the occurrence of P × E and performed a meta‐analysis to calculate the percentage of traits showing P × E in the study cases.ResultsWe identified 309 study cases (from 237 published articles) assessing population differentiation in 172 plant species, of which 64% included more than one test environment and tested P × E. In 77% of these studies, P × E was significant for at least one functional trait. The overall proportion of traits showing P × E was 33.4% (95% confidence interval 27.7–39.3). These results were generally consistent across life‐forms, ecoregions and type of experiment. Furthermore, population differentiation varied across test environments in 76% of cases. The overall proportion of traits showing environment‐dependent population differentiation was 53.7% (95% confidence interval 37.9–69.3).ConclusionsOur findings revealed that differences in phenotypic plasticity among populations are common but are usually neglected in order to forecast population responses to climate change. Future studies should assess population differentiation in many test environments (accounting for P × E) that realistically reflect future environmental conditions, assessing climate change drivers that are rarely considered (e.g., multifactor experiments incorporating higher CO2 levels). Our review also revealed the predominant focus of population studies on trees from temperate climates, identifying underexplored life‐forms (shrubs, annuals), phylogenetic groups (ferns, ancient gymnosperms) and ecoregions (tropical, arctic) that should receive more attention in future.
Development of vitamin E‐enriched functional foods: stability of tocotrienols in food systems
Authors: Pandya Janam K. , DeBonee Maryssa , Corradini Maria G. , Camire Mary Ellen , McClements David Julian , Kinchla Amanda J. 
Published: July 2019

SummaryTocotrienols are members of the vitamin E family that provide many nutritional benefits. Developing tocotrienol‐fortified functional foods introduces vitamin E into consumers diets without changing their food habits. The purpose of this study was to compare the stability of tocotrienols in bulk form and six food matrices at a fixed dose (40 mg kg−1) under accelerated and conventional storage conditions. The delta‐ and gamma‐tocotrienol content was measured using HPLC, and the sensory attributes were evaluated using triangle and hedonic tests. Tocotrienol in bulk powder was less stable under stressed conditions than in bulk oil, with the powders having approximately 40% and 80% reduction in δ‐ and γ‐ after storage and bulk oil with no significant degradation. HPLC analysis demonstrated that tocotrienol content remained unchanged in dry mix lemonade, yogurt, raw and UHT milk, and margarine over time; however, complete degradation of γ‐ and partial degradation (up to 50%) of δ‐tocotrienol was observed in bread during ambient and stressed storage conditions. A significant decrease in the δ‐tocotrienol relative concentration was also observed in HTST processed milk samples. Tocotrienol addition was not perceived, and no significant difference in the product attributes and overall liking was reported in the discrimination sensory testing.
Effect of dry fractionated hybrid protein ingredients on the structural, textural, thermal and sensory properties of gluten‐free oat and faba pasta
Authors: Duta Denisa E. , Culetu Alina , Sozer Nesli 
Published: July 2019

SummaryProtein‐enriched gluten‐free pasta was prepared from oat starch‐rich endosperm (SRE), which was substituted with different proportions of oat protein concentrate (OPC) and/or faba protein concentrate (FPC) fractions in order to obtain a similar protein content (18% and 35%). Accordingly, the health claims ‘source of protein’ and ‘high protein’ were achieved. Pasta with OPC and FPC had lower optimal cooking time (8 and 6.3 min), cooking loss (6% and 10.2% dm) and water absorption (152% and 147%) than control SRE pasta (9.3 min; 12.3% dm and 185%). Addition of protein concentrates produced an increase in hardness and chewiness of spaghetti. The glycaemic index of pasta was lowered by the addition of protein ingredients, the lowest value being achieved with FPC. In vitro protein digestibility of pasta increased up to 3.5% for OPC and up to 7.1% in case of FPC addition. The most protein‐digestible pasta was that with FPC addition to reach 35% protein content.
Genetic reprogramming for NK cell cancer immunotherapy with CRISPR/Cas9
Authors: Afolabi Lukman O. , Adeshakin Adeleye O. , Sani Musbahu M. , Bi Jiacheng , Wan Xiaochun 
Published: July 2019

SummaryNatural killer cells are potent cytotoxic lymphocytes specialized in recognizing and eliminating transformed cells, and in orchestrating adaptive anti‐tumour immunity. However, NK cells are usually functionally exhausted in the tumour microenvironment. Strategies such as checkpoint blockades are under investigation to overcome NK cell exhaustion in order to boost anti‐tumour immunity. The discovery and development of the CRISPR/Cas9 technology offer a flexible and efficient gene‐editing capability in modulating various pathways that mediate NK cell exhaustion, and in arming NK cells with novel chimeric antigen receptors to specifically target tumour cells. Despite the high efficiency in its gene‐editing capability, difficulty in the delivery of the CRISPR/Cas9 system remains a major bottleneck for its therapeutic applications, particularly for NK cells. The current review discusses feasible approaches to deliver the CRISPR/Cas9 systems, as well as potential strategies in gene‐editing for NK cell immunotherapy for cancers.
Research Review: Psychological and psychosocial treatments for children and young people with post‐traumatic stress disorder: a network meta‐analysis
Authors: Mavranezouli Ifigeneia , Megnin‐Viggars Odette , Daly Caitlin , Dias Sofia , Stockton Sarah , Meiser‐Stedman Richard , Trickey David , Pilling Stephen 
Published: July 2019

BackgroundPost‐traumatic stress disorder (PTSD) is a potentially chronic and disabling disorder that affects a significant minority of youth exposed to trauma. Previous studies have concluded that trauma‐focused cognitive behavioural therapy (TF‐CBT) is an effective treatment for PTSD in youth, but the relative strengths of different psychological therapies are poorly understood.MethodsWe undertook a systematic review and network meta‐analyses of psychological and psychosocial interventions for children and young people with PTSD. Outcomes included PTSD symptom change scores post‐treatment and at 1–4‐month follow‐up, and remission post‐treatment.ResultsWe included 32 trials of 17 interventions and 2,260 participants. Overall, the evidence was of moderate‐to‐low quality. No inconsistency was detected between direct and indirect evidence. Individual forms of TF‐CBT showed consistently large effects in reducing PTSD symptoms post‐treatment compared with waitlist. The order of interventions by descending magnitude of effect versus waitlist was as follows: cognitive therapy for PTSD (SMD −2.94, 95%CrI −3.94 to −1.95), combined somatic/cognitive therapies, child–parent psychotherapy, combined TF‐CBT/parent training, meditation, narrative exposure, exposure/prolonged exposure, play therapy, Cohen TF‐CBT/cognitive processing therapy (CPT), eye movement desensitisation and reprocessing (EMDR), parent training, group TF‐CBT, supportive counselling and family therapy (SMD −0.37, 95%CrI −1.60 to 0.84). Results for parent training, supportive counselling and family therapy were inconclusive. Cohen TF‐CBT/CPT, group TF‐CBT and supportive counselling had the largest evidence base. Results regarding changes in PTSD symptoms at follow‐up and remission post‐treatment were uncertain due to limited evidence.ConclusionsTrauma‐focused cognitive behavioural therapy, in particular individual forms, appears to be most effective in the management of PTSD in youth. EMDR is effective but to a lesser extent. Supportive counselling does not appear to be effective. Results suggest a large positive effect for emotional freedom technique, child–parent psychotherapy, combined TF‐CBT/parent training, and meditation, but further research is needed to confirm these findings as they were based on very limited evidence.
Immune landscape of hepatocellular carcinoma microenvironment: Implications for prognosis and therapeutic applications
Authors: Cariani Elisabetta , Missale Gabriele 
Published: July 2019

AbstractThe development of immunotherapy for solid tumours has boosted interest in the contexture of tumour immune microenvironment (TIME). Several lines of evidence indicate that the interplay between tumour cells and TIME components is a key factor for the evolution of hepatocellular carcinoma (HCC) and for the likelihood of response to immunotherapeutics. The availability of high‐resolution methods will be instrumental for a better definition of the complexity and diversity of TIME with the aim of predicting disease outcome, treatment response and possibly new therapeutic targets. Here, we review current knowledge about the immunological mechanisms involved in shaping the clinical course of HCC. Effector cells, regulatory cells and soluble mediators are discussed for their role defining TIME and as targets for immune modulation, together with possible immune signatures for optimization of immunotherapeutic strategies.
Why nurses should be Marxists
Authors: Porter Sam 
Published: July 2019

AbstractThe argument that nurses should be Marxists is made by looking at the primary areas of nursing activity in turn, giving an example of how capitalist economic relations negatively impact upon that activity, and providing a Marxist explanation of the reasons why it has that impact. In relation to the nursing activity of health promotion, it is argued that capitalism's generation of social inequality undermines the health of the population. In relation to curative activities, the focus is on how capitalism's reckless pursuit of profit has subverted the sustainability of bactericidal interventions. The argument in relation to comforting and assistive care is that the ownership and control of health services by capitalist corporations undermines therapeutic relationships. Finally, in relation to supportive care, it is contended that capitalism's requirement for a disciplined workforce can compromise healthcare professionals’ support of employees. It is concluded that if nurses aspire to have some control over their activities, then it is a good idea for them to avail of Marxism's capacity to identify the socio‐economic mechanisms by which capitalism affects nursing care.
Effect of apple cultivars and storage periods on the virulence of Neofabraea spp.
Authors: Di Francesco A. , Cameldi I. , Neri F. , Barbanti L. , Folchi A. , Spadoni A. , Baraldi E. 
Published: July 2019

Bull’s eye rot is a typical quiescent postharvest apple disease in major fruit‐growing areas. The susceptibility of different apple cultivars to Neofabraea spp. (N. vagabunda and N. malicorticis) was assessed, with Granny Smith showing the most resistance and Cripps Pink the most susceptibility. To assess the factors involved in conidial germination, Neofabraea spp. were grown on crude protein extracts (CPEs) collected from apple fruits at different storage periods. Fungal germ tube growth rate and pathogenic enzyme (cellulase and xylanase) activity were assessed. Results showed that CPEs collected after 2 and 4 months of storage progressively stimulated conidial germination and germ tube elongation, while a lesser effect was observed from CPEs after 1 month of storage. Xylanase proved to be the main degrading enzyme secreted by all the isolates, while cellulase was produced only by N. vagabunda isolates. Overall, the isolate ID02 was the most virulent, based on more rapid germ tube elongation and greater activity of the lytic enzymes.
Performance Modeling and Analysis of a Single-Shaft Closed-Cycle Gas Turbine Using Different Operational Control Strategy
Authors: Osigwe Emmanuel O. , Gad-Briggs Arnold , Igbong Dodeye , Nikolaidis Theoklis , Pilidis Pericles 
Published: July 2019

Abstract
In the last few years, one considerable factor for the viability and interest in the closed-cycle gas turbine (GT) systems for nuclear or conventional power plant application is its potential to maintain high cycle performance at varying operating conditions. However, for this potential to be realized, more competitive analysis and understanding of its control strategy are importantly required. In this paper, the iterative procedure for three independent control strategies of a 40 MW single-shaft intercooled-recuperated closed-cycle GT incorporated to a generation IV nuclear reactor is been analyzed and their performance at various operating conditions compared. The rationale behind this analysis was to explore different control strategies and to identify potential limitations using each independent control. The inventory control strategy offered a more viable option for high efficiency at changes in ambient and part-load operations, however, operational limitations in terms of size and pressure of inventory tank, rotational speed for which the centrifugal forces acting on the blade tips could become too high, hence would affect the mechanical integrity and compressor performance. The bypass control responds rapidly to load rejection in the event of loss of grid power. And more interestingly, the results showed the need for a mixed or combined control instead of a single independent technique, which is limited in practice due to operational limits.
Review of Morphing Laminated Composites
Authors: Chillara V. S. C. , Dapino M. J. 
Published: July 2019

Abstract
Morphing structures, defined as body panels that are capable of a drastic autonomous shape transformation, have gained importance in the aerospace, automotive, and soft robotics industries since they address the need to switch between shapes for optimal performance over the range of operation. Laminated composites are attractive for morphing because multiple laminae, each serving a specific function, can be combined to address multiple functional requirements such as shape transformation, structural integrity, safety, aerodynamic performance, and minimal actuation energy. This paper presents a review of laminated composite designs for morphing structures. The trends in morphing composites research are outlined and the literature on laminated composites is categorized based on deformation modes and multifunctional approaches. Materials commonly used in morphing structures are classified based on their properties. Composite designs for various morphing modes such as stretching, flexure, and folding are summarized and their performance is compared. Based on the literature, the laminae in an n-layered composite are classified based on function into three types: constraining, adaptive, and prestressed. A general analytical modeling framework is presented for composites comprising the three types of functional laminae. Modeling developments for each morphing mode and for actuation using smart material-based active layers are discussed. Results, presented for each deformation mode, indicate that the analytical modeling can not only provide insight into the structure's mechanics but also serve as a guide for geometric design and material selection.
Differences in access to Emergency Paediatric Intensive Care and care during Transport (DEPICT): study protocol for a mixed methods study
Authors: Ramnarayan Padmanabhan , Evans Ruth , Draper Elizabeth S , Seaton Sarah E , Wray Jo , Morris Stephen , Pagel Christina 
Published: July 2019

IntroductionFollowing centralisation of UK paediatric intensive care, specialist retrieval teams were established who travel to general hospitals to stabilise and transport sick children to regional paediatric intensive care units (PICUs). There is national variation among these PICU retrieval teams (PICRTs) in terms of how quickly they reach the patient’s bedside and in the care provided during transport. The impact of these variations on clinical outcomes and the experience of stakeholders (patients, families and healthcare staff) is however unknown. The primary objective of this study is to address this evidence gap.Methods and analysisThis mixed-methods project involves the following: (1) retrospective analysis of linked data from routine clinical audits (2014–2016) to assess the impact of service variations on 30-day mortality and other secondary clinical outcomes; (2) a prospective questionnaire study conducted at 24 PICUs and 9 associated PICRTs in England and Wales over a 12-month period in 2018 to collect experience data from parents of transported children as well as qualitative analysis of in-depth interviews with a purposive sample of patients, parents and staff to assess the impact of service variations on patient/family experience; (3) health economic evaluation analysing transport service costs (and other associated costs) against lives saved and longer term measurements of quality of life at 12 months in transported children and (4) mathematical modelling evaluating the costs and potential impact of different service configurations. A final work stream involves a series of stakeholder workshops to synthesise study findings and generate recommendations.Ethics and disseminationThe study has been reviewed and approved by the Health Research Authority, ref: 2 18 569. Study results will be actively disseminated through peer-reviewed journals, conference presentations, social media, print and broadcast media, the internet and stakeholder workshops.