bluuebunny/crossref_metadata_2025_split huggingface dataset:
Type-I hyperbolic metasurfaces for highly-squeezed designer polaritons with negative group velocity
Authors: Yang Yihao , Qin Pengfei , Lin Xiao , Li Erping , Wang Zuojia , Zhang Baile , Chen Hongsheng 
Published: May 2019

AbstractHyperbolic polaritons in van der Waals materials and metamaterial heterostructures provide unprecedented control over light-matter interaction at extreme nanoscales. Here we propose a concept of type-I hyperbolic metasurface supporting highly-squeezed magnetic designer polaritons, which act as magnetic analogs of hyperbolic polaritons in the hexagonal boron nitride (h-BN) in the first Reststrahlen band. Compared with the natural h-BN, the size and spacing of the metasurface unit cell can be readily engineered, allowing for manipulating designer polaritons in frequency and space with greater flexibility. Microwave experimental measurements display a cone-like dispersion in momentum space, exhibiting an effective refractive index up to 60 and a group velocity down to c/400. Tailoring the metasurface, we demonstrate an ultra-compact integrated designer polariton circuit including high-transmission 90° sharp bending waveguides and waveguide splitters. The present metasurface could serve as a platform for polaritonics, and find applications in waveguiding, terahertz sensing, subdiffraction focusing/imaging, low-threshold terahertz Cherenkov radiation, and wireless energy transfer.
Network analysis of synthesizable materials discovery
Authors: Aykol Muratahan , Hegde Vinay I. , Hung Linda , Suram Santosh , Herring Patrick , Wolverton Chris , Hummelshøj Jens S. 
Published: May 2019

AbstractAssessing the synthesizability of inorganic materials is a grand challenge for accelerating their discovery using computations. Synthesis of a material is a complex process that depends not only on its thermodynamic stability with respect to others, but also on factors from kinetics, to advances in synthesis techniques, to the availability of precursors. This complexity makes the development of a general theory or first-principles approach to synthesizability currently impractical. Here we show how an alternative pathway to predicting synthesizability emerges from the dynamics of the materials stability network: a scale-free network constructed by combining the convex free-energy surface of inorganic materials computed by high-throughput density functional theory and their experimental discovery timelines extracted from citations. The time-evolution of the underlying network properties allows us to use machine-learning to predict the likelihood that hypothetical, computer-generated materials will be amenable to successful experimental synthesis.
Black phosphorene as a hole extraction layer boosting solar water splitting of oxygen evolution catalysts
Authors: Zhang Kan , Jin Bingjun , Park Cheolwoo , Cho Yoonjun , Song Xiufeng , Shi Xinjian , Zhang Shengli , Kim Wooyul , Zeng Haibo , Park Jong Hyeok 
Published: May 2019

AbstractAs the development of oxygen evolution co-catalysts (OECs) is being actively undertaken, the tailored integration of those OECs with photoanodes is expected to be a plausible avenue for achieving highly efficient solar-assisted water splitting. Here, we demonstrate that a black phosphorene (BP) layer, inserted between the OEC and BiVO4 can improve the photoelectrochemical performance of pre-optimized OEC/BiVO4 (OEC: NiOOH, MnOx, and CoOOH) systems by 1.2∼1.6-fold, while the OEC overlayer, in turn, can suppress BP self-oxidation to achieve a high durability. A photocurrent density of 4.48 mA·cm−2 at 1.23 V vs reversible hydrogen electrode (RHE) is achieved by the NiOOH/BP/BiVO4 photoanode. It is found that the intrinsic p-type BP can boost hole extraction from BiVO4 and prolong holes trapping lifetime on BiVO4 surface. This work sheds light on the design of BP-based devices for application in solar to fuel conversion, and also suggests a promising nexus between semiconductor and electrocatalyst.
Hydrogen embrittlement in metallic nanowires
Authors: Yin Sheng , Cheng Guangming , Chang Tzu-Hsuan , Richter Gunther , Zhu Yong , Gao Huajian 
Published: May 2019

AbstractAlthough hydrogen embrittlement has been observed and extensively studied in a wide variety of metals and alloys, there still exist controversies over the underlying mechanisms and a fundamental understanding of hydrogen embrittlement in nanostructures is almost non-existent. Here we use metallic nanowires (NWs) as a platform to study hydrogen embrittlement in nanostructures where deformation and failure are dominated by dislocation nucleation. Based on quantitative in-situ transmission electron microscopy nanomechanical testing and molecular dynamics simulations, we report enhanced yield strength and a transition in failure mechanism from distributed plasticity to localized necking in penta-twinned Ag NWs due to the presence of surface-adsorbed hydrogen. In-situ stress relaxation experiments and simulations reveal that the observed embrittlement in metallic nanowires is governed by the hydrogen-induced suppression of dislocation nucleation at the free surface of NWs.
Structural mechanism underlying G protein family-specific regulation of G protein-gated inwardly rectifying potassium channel
Authors: Kano Hanaho , Toyama Yuki , Imai Shunsuke , Iwahashi Yuta , Mase Yoko , Yokogawa Mariko , Osawa Masanori , Shimada Ichio 
Published: May 2019

AbstractG protein-gated inwardly rectifying potassium channel (GIRK) plays a key role in regulating neurotransmission. GIRK is opened by the direct binding of the G protein βγ subunit (Gβγ), which is released from the heterotrimeric G protein (Gαβγ) upon the activation of G protein-coupled receptors (GPCRs). GIRK contributes to precise cellular responses by specifically and efficiently responding to the Gi/o-coupled GPCRs. However, the detailed mechanisms underlying this family-specific and efficient activation are largely unknown. Here, we investigate the structural mechanism underlying the Gi/o family-specific activation of GIRK, by combining cell-based BRET experiments and NMR analyses in a reconstituted membrane environment. We show that the interaction formed by the αA helix of Gαi/o mediates the formation of the Gαi/oβγ-GIRK complex, which is responsible for the family-specific activation of GIRK. We also present a model structure of the Gαi/oβγ-GIRK complex, which provides the molecular basis underlying the specific and efficient regulation of GIRK.
Targeting the HIV-infected brain to improve ischemic stroke outcome
Authors: Bertrand Luc , Méroth Fannie , Tournebize Marie , Leda Ana Rachel , Sun Enze , Toborek Michal 
Published: May 2019

AbstractHIV-associated cerebrovascular events remain highly prevalent even in the current era of antiretroviral therapy (ART). We hypothesize that low-level HIV replication and associated inflammation endure despite antiretroviral treatment and affect ischemic stroke severity and outcomes. Using the EcoHIV infection model and the middle cerebral artery occlusion as the ischemic stroke model in mice, we present in vivo analysis of the relationship between HIV and stroke outcome. EcoHIV infection increases infarct size and negatively impacts tissue and functional recovery. Ischemic stroke also results in an increase in EcoHIV presence in the affected regions, suggesting post-stroke reactivation that magnifies pro-inflammatory status. Importantly, ART with a high CNS penetration effectiveness (CPE) is more beneficial than low CPE treatment in limiting tissue injury and accelerating post-stroke recovery. These results provide potential insight for treatment of HIV-infected patients that are at risk of developing cerebrovascular disease, such as ischemic stroke.
Meta-analytic evidence that sexual selection improves population fitness
Authors: Cally Justin G. , Stuart-Fox Devi , Holman Luke 
Published: May 2019

AbstractSexual selection has manifold ecological and evolutionary consequences, making its net effect on population fitness difficult to predict. A powerful empirical test is to experimentally manipulate sexual selection and then determine how population fitness evolves. Here, we synthesise 459 effect sizes from 65 experimental evolution studies using meta-analysis. We find that sexual selection on males tends to elevate the mean and reduce the variance for many fitness traits, especially in females and in populations evolving under stressful conditions. Sexual selection had weaker effects on direct measures of population fitness such as extinction rate and proportion of viable offspring, relative to traits that are less closely linked to population fitness. Overall, we conclude that the beneficial population-level consequences of sexual selection typically outweigh the harmful ones and that the effects of sexual selection can differ between sexes and environments. We discuss the implications of these results for conservation and evolutionary biology.
Plasmonic photosynthesis of C1–C3 hydrocarbons from carbon dioxide assisted by an ionic liquid
Authors: Yu Sungju , Jain Prashant K. 
Published: May 2019

AbstractPhotochemical conversion of CO2 into fuels has promise as a strategy for storage of intermittent solar energy in the form of chemical bonds. However, higher-energy-value hydrocarbons are rarely produced by this strategy, because of kinetic challenges. Here we demonstrate a strategy for green-light-driven synthesis of C1–C3 hydrocarbons from CO2 and H2O. In this approach, plasmonic excitation of Au nanoparticles produces a charge-rich environment at the nanoparticle/solution interface conducive for CO2 activation, while an ionic liquid stabilizes charged intermediates formed at this interface, facilitating multi-step reduction and C–C coupling. Methane, ethylene, acetylene, propane, and propene are photosynthesized with a C2+ selectivity of ~50% under the most optimal conditions. Hydrocarbon turnover exhibits a volcano relationship as a function of the ionic liquid concentration, the kinetic analysis of which coupled with density functional theory simulations provides mechanistic insights into the synergy between plasmonic excitation and the ionic liquid.
Recent advances in the production of recombinant glycoconjugate vaccines
Authors: Kay Emily , Cuccui Jon , Wren Brendan W. 
Published: May 2019

AbstractGlycoconjugate vaccines against bacteria are one of the success stories of modern medicine and have led to a significant reduction in the global occurrence of bacterial meningitis and pneumonia. Glycoconjugate vaccines are produced by covalently linking a bacterial polysaccharide (usually capsule, or more recently O-antigen), to a carrier protein. Given the success of glycoconjugate vaccines, it is surprising that to date only vaccines againstHaemophilus influenzaetype b,Neisseria meningitisandStreptococcus pneumoniaehave been fully licenced. This is set to change through the glycoengineering of recombinant vaccines in bacteria, such asEscherichia coli, that act as mini factories for the production of an inexhaustible and renewable supply of pure vaccine product. The recombinant process, termed Protein Glycan Coupling Technology (PGCT) or bioconjugation, offers a low-cost option for the production of pure glycoconjugate vaccines, with the in-built flexibility of adding different glycan/protein combinations for custom made vaccines. Numerous vaccine candidates have now been made using PGCT, which include those improving existing licenced vaccines (e.g., pneumococcal), entirely new vaccines for both Gram-positive and Gram-negative bacteria, and (because of the low production costs) veterinary pathogens. Given the continued threat of antimicrobial resistance and the potential peril of bioterrorist agents, the production of new glycoconjugate vaccines against old and new bacterial foes is particularly timely. In this review, we will outline the component parts of bacterial PGCT, including recent advances, the advantages and limitations of the technology, and future applications and perspectives.
In-planta expression of insecticidal proteins provides protection against lepidopteran insects
Authors: Rauf Imran , Javaid Shaista , Naqvi Rubab Zahra , Mustafa Tanveer , Amin Imran , Mukhtar Zahid , Jander Georg , Mansoor Shahid 
Published: May 2019

AbstractThe development of advanced biotechnological control strategies opens a new era of environment friendly pest management. The current study is part of such an effort, in which we developed a control strategy based on gene pyramiding that confers broad-spectrum resistance against lepidopteran (Helicoverpa armigera and Spodoptera litura) and hemipteran (Myzus persicae, Phenacoccus solenopsis, and Bemisia tabaci) insect pests. Previously, we reported a double gene construct expressing Hvt and lectin in tobacco (Nicotiana tabacum) plants under phloem specific promoters which confers resistance against hemipteran insects. Here we extended our studies by evaluating the advanced generation of these tobacco plants expressing hvt-lectin against lepidopteran insects. Tobacco plants expressing both toxins were tested against H. armigera and S. litura. Insect bioassay results showed 100% mortality of H. armigera within 48–72 hours and 100% mortality of S. litura within 72–96 hours. Our results suggest that the use of both toxins as a gene pyramiding strategy to control both lepidopteran and hemipterans insects on commercial basis to reduce the use of chemical pesticides.
Mouse models and strain-dependency of Chédiak-Higashi syndrome-associated neurologic dysfunction
Authors: Hedberg-Buenz Adam , Dutca Laura M. , Larson Demelza R. , Meyer Kacie J. , Soukup Dana A. , van der Heide Carly J. , Mercer Hannah E. , Wang Kai , Anderson Michael G. 
Published: May 2019

AbstractChédiak-Higashi syndrome (CHS) is a lethal disorder caused by mutations in the LYST gene that involves progressive neurologic dysfunction. Lyst-mutant mice exhibit neurologic phenotypes that are sensitive to genetic background. On the DBA/2J-, but not on the C57BL/6J-background, Lyst-mutant mice exhibit overt tremor phenotypes associated with loss of cerebellar Purkinje cells. Here, we tested whether assays for ataxia could measure this observed strain-dependency, and if so, establish parameters for empowering phenotype- and candidate-driven approaches to identify genetic modifier(s). A composite phenotypic scoring system distinguished phenotypes in Lyst-mutants and uncovered a previously unrecognized background difference between wild-type C57BL/6J and DBA/2J mice. Accelerating rotarod performance also distinguished phenotypes in Lyst-mutants, but at more advanced ages. These results establish that genetic background, Lyst genotype, and age significantly influence the severity of CHS-associated neurologic deficits. Purkinje cell quantifications likewise distinguished phenotypes of Lyst-mutant mice, as well as background differences between wild-type C57BL/6J and DBA/2J mice. To aid identification of potential genetic modifier genes causing these effects, we searched public datasets for cerebellar-expressed genes that are differentially expressed and/or contain potentially detrimental genetic variants. From these approaches, Nos1, Prdx2, Cbln3, Gnb1, Pttg1 were confirmed to be differentially expressed and leading candidates.
RNA-seq analysis of the salivary glands and midgut of the Argasid tick Ornithodoros rostratus
Authors: Araujo Ricardo N. , Silva Naylene C. S. , Mendes-Sousa Antonio , Paim Rafaela , Costa Gabriel C. A. , Dias Luciana R. , Oliveira Karla , Sant’Anna Mauricio R. V. , Gontijo Nelder F. , Pereira Marcos H. , Pessoa Grasielle D. , Valenzuela Jesus G. , Koerich Leonardo B. , Oliveira Fabiano 
Published: May 2019

AbstractOrnithodoros rostratus is a South American argasid tick which importance relies on its itchy bite and potential as disease vector. They feed on a wide variety of hosts and secrete different molecules in their saliva and intestinal content that counteract host defences and help to accommodate and metabolize the relatively large quantity of blood upon feeding. The present work describes the transcriptome profile of salivary gland (SG) and midgut (MG) of O. rostratus using Illumina sequencing. A total of 8,031 contigs were assembled and assigned to different functional classes. Secreted proteins were the most abundant in the SG and accounted for ~67% of all expressed transcripts with contigs with identity to lipocalins and acid tail proteins being the most representative. On the other hand, immunity genes were upregulated in MG with a predominance of defensins and lysozymes. Only 10 transcripts in SG and 8 in MG represented ~30% of all RNA expressed in each tissue and one single contig (the acid tail protein ORN-9707) represented ~7% of all expressed contigs in SG. Results highlight the functional difference of each organ and identified the most expressed classes and contigs of O. rostratus SG and MG.
Comparison of livestock-associated and community-associated Staphylococcus aureus pathogenicity in a mouse model of skin and soft tissue infection
Authors: Randad Pranay R. , Dillen Carly A. , Ortines Roger V. , Mohr David , Aziz Maliha , Price Lance B. , Kaya Hülya , Larsen Jesper , Carroll Karen C. , Smith Tara C. , Miller Lloyd S. , Heaney Christopher D. 
Published: May 2019

AbstractIndustrial hog operation (IHO) workers are at increased risk of carrying Staphylococcus aureus in their nares, particularly strains that are livestock-associated (LA) and multidrug-resistant. The pathogenicity of LA-S. aureus strains remains unclear, with some prior studies suggesting reduced transmission and virulence in humans compared to community-associated methicillin-resistant (CA-MRSA) S. aureus. The objective of this study was to determine the degree to which LA-S. aureus strains contracted by IHO workers cause disease relative to a representative CA-MRSA strain in a mouse model of skin and soft tissue infection (SSTI). Mice infected with CC398 LA-S. aureus strains (IHW398-1 and IHW398-2) developed larger lesion sizes with higher bacterial burden than mice infected with CA-MRSA (SF8300) (p < 0.05). The greatest lesion size and bacterial burden was seen with a CC398 strain that produced a recurrent SSTI in an IHO worker. The LA-S. aureus infected mice had decreased IL-1β protein levels compared with CA-MRSA-infected mice (p < 0.05), suggesting a suboptimal host response to LA-S. aureus SSTIs. WGSA revealed heterogeneity in virulence factor and antimicrobial resistance genes carried by LA-S. aureus and CA-MRSA strains. The observed pathogenicity suggest that more attention should be placed on preventing the spread of LA-S. aureus into human populations.
Serum Concentrations of Citrate, Tyrosine, 2- and 3- Hydroxybutyrate are Associated with Increased 3-Month Mortality in Acute Heart Failure Patients
Authors: Stryeck Sarah , Gastrager Michaela , Degoricija Vesna , Trbušić Matias , Potočnjak Ines , Radulović Bojana , Pregartner Gudrun , Berghold Andrea , Madl Tobias , Frank Saša 
Published: May 2019

AbstractConsidering the already established relationship between the extent of the metabolic dysfunction and the severity of heart failure (HF), it is conceivable that the metabolomic profile of the serum may have a prognostic capacity for 3-month mortality in acute heart failure (AHF). Out of 152 recruited patients, 130 serum samples were subjected to the metabolomic analyses. The 3-month mortality rate was 24.6% (32 patients). Metabolomic profiling by nuclear magnetic resonance spectroscopy found that the serum levels of 2-hydroxybutyrate (2-HB), 3-hydoxybutyrate (3-HB), lactate, citrate, and tyrosine, were higher in patients who died within 3 months compared to those who were alive 3 months after onset of AHF, which was confirmed by univariable logistic regression analyses (p = 0.009, p = 0.005, p = 0.008, p<0.001, and p<0.001, respectively). These associations still remained significant for all tested metabolites except for lactate after adjusting for established prognostic parameters in HF. In conclusion, serum levels of 2-HB, 3-HB, tyrosine, and citrate measured at admission are associated with an increased 3-month mortality rate in AHF patients and might thus be of prognostic value in AHF.
Anaerobiosis influences virulence properties of Pseudomonas aeruginosa cystic fibrosis isolates and the interaction with Staphylococcus aureus
Authors: Pallett Ross , Leslie Laura J. , Lambert Peter. A. , Milic Ivana , Devitt Andrew , Marshall Lindsay J. 
Published: May 2019

AbstractThe airways of individuals with cystic fibrosis (CF) are abundantly colonised by Staphylococcus aureus and Pseudomonas aeruginosa. Co-infecting hypoxic regions of static mucus within CF airways, together with decreases in pulmonary function, mucus plugging and oxygen consumption by host neutrophils gives rise to regions of anoxia. This study determined the impact of anaerobiosis upon S. aureus-P. aeruginosa interactions in planktonic co-culture and mixed species biofilms in vitro. Whilst anoxia reduced the ability for P. aeruginosa CF isolates to dominate over S. aureus, this occurred in an isolate dependent manner. Investigations into the underlying mechanisms suggest that the anti-staphylococcal compound facilitating P. aeruginosa dominance under normoxia and anoxia is greater than 3 kDa in size and is heat-stable. Not all interspecies interactions studied were antagonistic, as S. aureus exoproducts were shown to restore and enhance P. aeruginosa motility under normoxia and anoxia in an isolate dependent manner. Collectively, this study suggests changes in oxygen availability within regions of the CF lung is likely to influence interspecies interactions and in turn, potentially influence disease progression.
Studies on Isoniazid Derivatives through a Medicinal Chemistry Approach for the Identification of New Inhibitors of Urease and Inflammatory Markers
Authors: Rizvi Fazila , Khan Majid , Jabeen Almas , Siddiqui Hina , Choudhary M. Iqbal 
Published: May 2019

AbstractA library of thiosemicarbazide derivatives of isoniazid 3–27, was synthesized and evaluated for their anti-inflammatory and urease inhibition activities, by using in vitro bioassays. Among these compounds 9, 10, 12, 21, and 26 were identified as new derivatives. Prolonged use of non-steroidal anti-inflammatory drugs (NSAIDs) and infections caused by Helicobacter pylori (ureolytic bacteria), are the two most significant causes of gastric and peptic ulcers. We focused on the identification of the dual inhibitors of inflammation and urease enzyme. Compound 23 was identified as the best dual inhibitor of inflammation (ROS; IC50 = 12.3 µg/mL), and urease enzyme inhibition activity (IC50 = 22.4 µM). Many of these compounds showed comparable activities to the standard anti-inflammatory drug (ibuprofen, IC50 = 11.2 µg/mL) and urease inhibitor (thiourea/acetohydraoxamic acid, IC50 = 21.1/20.3 µM). Compound 12 was found to be the most potent urease inhibitor (IC50 = 12.3 µM) and good inhibitor of inflammation (IC50 = 27.7 µg/mL). Compounds 19, 11, 13, 9, 17, 10, and 16, were also found to be potent inhibitors of urease. Cytotoxicity was also evaluated and all the compounds were found to be non-cytotoxic, except compound 18 and the parent drug isoniazid (IC50 = 29.5 and 28.5 µM, respectively).