bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic basis and outcome in a nationwide study of Finnish patients with hypertrophic cardiomyopathy
Authors: Jääskeläinen Pertti , Vangipurapu Jagadish , Raivo Joose , Kuulasmaa Teemu , Heliö Tiina , Aalto‐Setälä Katriina , Kaartinen Maija , Ilveskoski Erkki , Vanninen Sari , Hämäläinen Liisa , Melin John , Kokkonen Jorma , Nieminen Markku S. ,   The FinHCM Study Group, Laakso Markku , Kuusisto Johanna 
Published: February 2019

AbstractAimsNationwide large‐scale genetic and outcome studies in cohorts with hypertrophic cardiomyopathy (HCM) have not been previously published.Methods and resultsWe sequenced 59 cardiomyopathy‐associated genes in 382 unrelated Finnish patients with HCM and found 24 pathogenic or likely pathogenic mutations in six genes in 38.2% of patients. Most mutations were located in sarcomere genes (MYBPC3, MYH7, TPM1, and MYL2). Previously reported mutations by our study group (MYBPC3‐Gln1061Ter, MYH7‐Arg1053Gln, and TPM1‐Asp175Asn) and a fourth major mutation MYH7‐Val606Met accounted for 28.0% of cases. Mutations in GLA and PRKAG2 were found in three patients. Furthermore, we found 49 variants of unknown significance in 31 genes in 20.4% of cases. During a 6.7 ± 4.2 year follow‐up, annual all‐cause mortality in 482 index patients and their relatives with HCM was higher than that in the matched Finnish population (1.70 vs. 0.87%; P < 0.001). Sudden cardiac deaths were rare (n = 8). Systolic heart failure (hazard ratio 17.256, 95% confidence interval 3.266–91.170, P = 0.001) and maximal left ventricular wall thickness (hazard ratio 1.223, 95% confidence interval 1.098–1.363, P < 0.001) were independent predictors of HCM‐related mortality and life‐threatening cardiac events. The patients with a pathogenic or likely pathogenic mutation underwent an implantable cardioverter defibrillator implantation more often than patients without a pathogenic or likely pathogenic mutation (12.9 vs. 3.5%, P < 0.001), but there was no difference in all‐cause or HCM‐related mortality between the two groups. Mortality due to HCM during 10 year follow‐up among the 5.2 million population of Finland was studied from death certificates of the National Registry, showing 269 HCM‐related deaths, of which 32% were sudden.ConclusionsWe identified pathogenic and likely pathogenic mutations in 38% of Finnish patients with HCM. Four major sarcomere mutations accounted for 28% of HCM cases, whereas HCM‐related mutations in non‐sarcomeric genes were rare. Mortality in patients with HCM exceeded that of the general population. Finally, among 5.2 million Finns, there were at least 27 HCM‐related deaths annually.
Comparison of false-positive rates of 2 hypothesis-test approaches in relation to laboratory toxicity test performance
Authors: Fox John F. , Denton Debra L. , Diamond Jerry , Stuber Robyn 
Published: February 2019

Abstract

We compared 2 statistical hypothesis-test approaches (no-observed-effect concentration [NOEC] and test of significant toxicity [TST]) to determine the influence of laboratory test performance on the false-positive error rate using the US Environmental Protection Agency's Ceriodaphnia dubia reproduction whole-effluent toxicity (WET) test endpoint. Simulation and power calculations were used to determine error rates based on observed control coefficients of variation (CV) for 8 laboratories over a range of effect levels. Average C. dubia control reproduction among laboratories was 20 to 40 offspring per female, and the 75th percentile CV was 0.10 to 0.31, reflecting a range in laboratory performance. The 2 approaches behave similarly for CVs of 0.2 to 0.3. At effects &lt;10%, as CV decreases, TST is less likely to declare toxicity and NOEC is more likely to do so. Laboratory performance affects whether a sample is declared toxic and influences the probability of false-positive (and −negative) error rates using either approach. At the 75th percentile control CV observed for each laboratory, 4 laboratories would achieve approximately a 5% false-positive rate using 13 or fewer replicates for this test method. For the remaining 4 laboratories, more replicates would be needed to achieve a 5% false-positive rate. The present analyses demonstrate how false-positive rates are influenced by laboratory performance and WET test design. Environ Toxicol Chem 2019;38:511–523. Published 2019 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.
Performance analysis of multicell massive MIMO THP with pilot contamination
Authors: Maboud Sanaie Saiedeh , Khaleghi Bizaki Hossein 
Published: February 2019

AbstractPilot contamination (PC) is a performance limiting factor in multicell massive multiple‐input–multiple‐output systems. The PC may cause the channel estimation of users in the desired cell to be polluted with the channels of users with the same set of pilots in the adjacent cells. In this paper, we propose a multicell Tomlinson‐Harashima precoding (MC‐THP) to mitigate PC. The MC‐THP matrices are derived by the optimization function in which the errors seen by the users in the desired cell and the interference seen by the users in the neighboring cells are jointly minimized. In addition, the robust MC‐THP design with imperfect channel state information is investigated based on the covariance matrices of the channel estimation errors of the desired and interfering channels. Finally, an approximate analytical closed‐form of symbol error rate is obtained through the analysis of error covariance matrix in the desired cell. This is then used for power loading technique to improve the performance of the proposed MC‐THP. The simulation results confirm the improvement of the proposed MC‐THP concerning the conventional linear multicell minimum mean‐square error (MC‐MMSE) precoder.
On the capacity of additive white mixture Gaussian noise channels
Authors: Sheikh‐Hosseini Mohsen , Hodtani Ghosheh Abed 
Published: February 2019

AbstractThis paper provides an information‐theoretic study for the additive white impulsive noise channels by considering Gaussian mixture (GM) noise model, which includes Middleton class A andε‐mixture Gaussian models. First, three lower and two upper bounds are found for the differential entropy of complex multivariate GM distribution and these bounds are extended to the univariate GM noise of flat fading impulsive noise channels. Second, the instantaneous capacity of these channels is bounded using two lower and two upper bounds, and the ergodic capacity and outage probability of these bounds are investigated for the log‐normal fading model. Finally, the obtained bounds are numerically illustrated and their tightness is discussed for various realizations of the GM noise. The results demonstrate that the tight bounds are not the same for all considered realizations, but they are close enough and converge to each other for a specific realization.
Production and characterization of a new glycolipid, mannosylerythritol lipid, from waste cooking oil biotransformation by Pseudozyma aphidis ZJUDM34
Authors: Niu Yongwu , Wu Jianan , Wang Wei , Chen Qihe 
Published: February 2019

AbstractMannosylerythritol lipids (MELs) are glycolipids possessing unique biosurfactant properties. However, the prices of substrates currently used for MEL formation caused its unsustainable commercial development. Waste cooking oil poses significant ecological and economical problems. Thus, the production of MELs from used waste cooking oil using the biotransformation route is one of the better alternatives to utilize it efficiently and economically. This work aims at the production of MELs using waste cooking oil instead of soybean oil and evaluating the major characteristics and compositions of MELs. The titers reached 61.50 g/L by the optimization of culture medium, higher than the counterpart (10.25 ± 0.32 g/L) of the nonoptimized medium. MELs exhibited good surface activity and better performance in contrast to MELs grown on soybean oil. The water phase behavior of MEL‐A was also evaluated. The process showed higher productivity of MELs with better surface activity and application stability than the conventional process using soybean oil. The findings of this study imply that the use of inexpensive fermentation substrates associated with straightforward downstream processing is expected to have a great impact on the economy of MEL production.
Long noncoding RNA DINO (damage induced noncoding) represses the development of gastric cancer by modulating p21 and Bcl‐2 Associated X Protein (Bax) expression
Authors: Liu Qinqiang , Xiao Yan , Cai Peng , Li Jun , Li Dawei 
Published: February 2019

AbstractlncRNAs are responsible for a variety of diseases, including gastric cancer (GC). Many recent studies have reported that lncRNAs can serve as crucial regulators of various genes. Nevertheless, the biological function of lncRNA damage induced noncoding (DINO) remained poorly investigated in GC. Therefore, in our present study, the detailed role of DINO was investigated. It was manifested that DINO was significantly downregulated in GC tissues. Then, DINO was modulated by infecting LV‐DINO or by LV‐shRNA in BGC‐823 and MGC‐803 cells. Moreover, it was displayed that GC cell proliferation was suppressed by DINO overexpression, whereas silencing DINO increased cell proliferation significantly. For another, it was indicated that DINO dramatically induced apoptotic ratios of BGC‐823 and MGC‐803 cells, whereas the decrease of DINO depressed GC cell apoptosis. Apart from these, GC cell cycle progression was greatly blocked by LV‐DINO. Furthermore, Western blot results displayed that upregulation of DINO elevated p21 expression and Bax expression. Oppositely, inhibition of DINO greatly suppressed p21 and Bax protein expression level. Taken these, DINO might exert a tumor inhibitory role in the progression of GC through modulating p21 and Bax.
Mesenchymal stem cells improve platelet counts in mice with immune thrombocytopenia
Authors: Zhang Ping , Zhang Guoyang , Liu Xiaoyan , Liu Hongyun , Yang Pengfeng , Ma Liping 
Published: February 2019

AbstractImmune thrombocytopenia (ITP) is a common autoimmune bleeding disorder. The breakdown of immune tolerance (regulatory T [Treg] cells and suppressor cytokines) plays an important role in ITP pathophysiology, especially in refractory ITP. Bone marrow–derived mesenchymal stem cells (BM‐MSCs) show immunomodulatory properties and have been extensively utilized for autoimmune diseases. However, it has not been fully elucidated how BM‐MSCs affect ITP. In this study, we explore the therapeutic mechanism of BM‐MSCs on ITP in mice. Dose‐escalation passive ITP mice were inducted by injection of MWReg30. BALB/c mice were randomly divided into two groups: ITP with BM‐MSC transplantation and ITP controls. The serum levels of cytokines (interleukin 10 [IL‐10] and transforming growth factor‐β1 [TGF‐β1]) were examined by enzyme‐linked immunosorbent assays. The frequency of Treg cells in both peripheral blood and spleen mononuclear cells was analyzed by flow cytometry, and the forkhead box P3 (Foxp3) messenger RNA (mRNA) level was measured by real‐time polymerase chain reaction. After BM‐MSC treatment, the platelet (PLT) counts were significantly elevated. Meanwhile, cytokines (TGF‐β1 and IL‐10), the ratios of Treg cells, and the Foxp3 mRNA expression level were significantly higher in the BM‐MSC group. Our results show that BM‐MSCs can improve PLT counts mainly by secreting suppressive cytokines and upregulating Tregs, which may provide new therapeutic potential for human ITP.
Exonuclease 1 expression is associated with clinical progression, metastasis, and survival prognosis of prostate cancer
Authors: Luo Fei , Wang Yu‐Zhuo , Lin Dong , Li Jian , Yang Kuo 
Published: February 2019

AbstractProstate cancer (PCa) is the most prevalent malignancy and the second leading cause of cancer‐related deaths in the male population in western countries, and we explored the association between exonuclease 1 (EXO1) expression and clinical progression, metastasis (Met), and survival prognosis of PCa. EXO1 expression of high/low‐metastatic patient‐derived xenografts model was investigated and clinical correlation and prognosis outcomes were validated. EXO1 in high‐metastatic models was significantly increased compared with low‐metastatic lines. In memorial sloan‐kettering cancer center (MSKCC) cohort, EXO1 expression positively correlated with PCa Met, and patients with high EXO1 had poor biochemical recurrence‐free survival in primary PCa cohort. Validation in The Cancer Genome Atlas primary cohort indicated EXO1 expression was significantly associated with lymph node Met and disease‐free survival. The overexpression of EXO1 is significantly associated with PCa poor survival outcome, and is a promising biomarker for PCa, especially for primary PCa. A prospective study is clearly needed to validate these findings.
Prognostic genes of hepatocellular carcinoma based on gene coexpression network analysis
Authors: Xu Baojin , Lv Wu , Li Xiaoyan , Zhang Lina , Lin Jie 
Published: February 2019

AbstractHepatocellular carcinoma (HCC) is the most common subtype in liver cancer whose prognosis is affected by malignant progression associated with complex gene interactions. However, there is currently no available biomarkers associated with HCC progression in clinical application. In our study, RNA sequencing expression data of 50 normal samples and 374 tumor samples was analyzed and 9225 differentially expressed genes were screened. Weighted gene coexpression network analysis was then conducted and the blue module we were interested was identified by calculating the correlations between 17 gene modules and clinical features. In the blue module, the calculation of topological overlap was applied to select the top 30 genes and these 30 genes were divided into the green group (11 genes) and the yellow group (19 genes) through searching whether these genes were validated by in vitro or in vivo experiments. The genes in the green group which had never been validated by any experiments were recognized as hub genes. These hub genes were subsequently validated by a new data set GSE76427 and KM Plotter Online Tool, and the results indicated that 10 genes (FBXO43, ARHGEF39, MXD3, VIPR1, DNASE1L3, PHLDA1, CSRNP1, ADR2B, C1RL, and CDC37L1) could act as prognosis and progression biomarkers of HCC. In summary, 10 genes who have never been mentioned in HCC were identified to be associated with malignant progression and prognosis of patients. These findings may contribute to the improvement of the therapeutic decision, risk stratification, and prognosis prediction for HCC patients.
Adverse impact of carbon tetrachloride on metabolic function in mice
Authors: Liang Xiaoliu , Wu Ka , Liu Meizhen , Yang Bin 
Published: February 2019

AbstractCarbon tetrachloride (CCl4), a potent hepatotoxin, is linked to the histopathological outcomes of inflammatory or oxidative stress, and cell death. However, further study of additional dysmetabolism induced by CCl
4 toxicant has not yet been investigated. In current study, chronical and acute exposures of CCl
4 in mice were used to unmask the biological molecular mechanism responsible for insulin‐dependent metabolic disorder. In experimental methods, a number of biochemical assays were used in assessment of biological impacts on insulin‐produced pancreas and insulin‐responsive hepatocyte after long‐ and short‐term exposures of CCl
4 toxicant, respectively. As a result, data from oral glucose tolerance test showed that CCl
4 exposures induced glucose tolerance and disrupted blood insulin and glucagon levels time‐dependently. Meanwhile, biochemical and histocytological analyses further indicated that CCl
4 exposures significantly resulted in liver cell damage, induced abnormal changes of hepatic and skeletal glycogen synthesis. In addition, acute CCl
4‐exposed mice showed reduced functional proteins of glucose transporter 2 (GLUT2), insulin receptor β, insulin receptor substrate 1, glycogen synthase kinase 3β (GSK3β), p‐AKT
Ser473 associated with AKT signaling pathway in liver cells, whereas acute CCl
4 exposure downregulated the endogenous expressions of the insulin and glucagon hormonal proteins in the pancreas. Taken together, the current findings highlight that CCl
4 impaired insulin‐dependent glucose homeostasis through modulating hepatocellular AKT signaling pathway in acute CCl
4 exposure and GLUT2/GSK3β pathway in chronic CCl
4‐exposed liver cells.
Continuous flow adsorption of phenolic compounds from olive mill wastewater with resin XAD16N: life cycle assessment, cost–benefit analysis and process optimization
Authors: Frascari Dario , Molina Bacca Aurora Esther , Wardenaar Tjerk , Oertlé Emmanuel , Pinelli Davide 
Published: February 2019

AbstractBACKGROUNDOlive mill wastewaters (OMWs) represent a major environmental concern due to their high organic load and phytotoxic activity. The selective recovery of phenolic compounds (PCs) from OMW is promising, thanks to the antioxidant and antimicrobial properties of PCs. The goal of this work was to perform a life cycle assessment (LCA) and cost–benefit analysis (CBA) of a full‐scale process of PC adsorption/desorption on resin Amberlite XAD16N. The industrial process was designed on the basis of laboratory tests aimed at performing a preliminary process optimization.RESULTSAdsorption tests were conducted at different velocities in a 1.8‐m column packed with XAD16N. The optimal superficial velocity and retention time (2.78 m h–1 and 0.56 h) allowed the attainment of satisfactory performances in terms of resin operating capacity (0.46), PC adsorption yield (0.92), PC mass fraction in the sorbed product (0.50 gPC/gVS) and specific antioxidant activity (3–6 gascorbic acid/gPC). Six consecutive adsorption/desorption cycles, operated with the same resin load, resulted in stable process performances. The LCA indicated that the environmental impact of the process could be decreased markedly through the addition of an anaerobic digestion step for the production of irrigation‐quality water and fertilizers from the dephenolized OMW. The PC market price required for the generation of a positive business case resulted relatively low (€1.7–13.5 kgPC–1).CONCLUSIONThe results indicate that the proposed PC adsorption/desorption technology, if integrated with an anaerobic digestion step, represents a promising solution for the treatment and valorization of OMW, a major agro‐industrial waste in Mediterranean countries. © 2019 Society of Chemical Industry
Modeling the clay minerals–enzyme binding by fusion fluorescent proteins and under atomic force microscope
Authors: Math Renukaradhya K. , Reddy Srinivasa , Dae Yun Han , Kambiranda Devaiah , Ghebreiyessus Yemane 
Published: February 2019

AbstractIn the present study, binding of cellulase protein to different clay minerals were tested using fluorescent–protein complex and microscopic techniques. Cellulase gene (Cel5H) was cloned into three fluorescent vectors and expressed as fusion enzymes. Binding of Cel5H–mineral particles was confirmed by confocal microscopy, and enzyme assay. Among the Cel5H–fusion enzymes, green–fusion enzyme showed higher intensity compared with other red and yellow fusion–proteins. Intensity of fusion–proteins was dependent on the pH of the medium. Confocal microscopy revealed binding of the all three fusion proteins with different clay minerals. However, montmorillonite displayed higher binding capacity than kaolinite clay. Likewise, atomic force microscopy (AFM) image profile analysis showed proteins appeared globular molecules in free‐state on mica surface with an average cross sectional diameter of 110 ± 2 nm and rough surface of montmorillonite made protein appear flattened due to structural alteration. Even surface of kaolinite also exerted some strain on protein molecular conformation after binding to surface. Our results provide further evidence for 3D visualization of enzyme–soil complex and encourage furthering study of the force involved interactions. Therefore, our results indicate that binding of proteins to clay minerals was external and provides a molecular method to observe the interaction of clay minerals–enzyme complex.
Policies towards donation after circulatory death liver transplantation: the need for a guideline?
Authors: van Reeven Marjolein , Pirenne Jacques , Muiesan Paolo , Fondevila Constantino , IJzermans Jan N. M. , Polak Wojciech G. 
Published: February 2019

AbstractBackgroundLiver transplantation (LT) using grafts from donation after circulatory death (DCD) is evolving to standard of care in many countries. Various transplant centers have developed a protocol for DCD‐LT. The existence of numerous protocols may cause inconsistencies. Knowledge of these differences may help improve the outcome of DCD‐LT.MethodsAn internet‐based survey was sent to 119 transplant surgeons among four countries: Belgium (BE), the Netherlands (NL), Spain (ES) and the United Kingdom (UK).ResultsThirty‐three percent of all respondents indicated having no specific age limit for DCD‐LT donors, and if there was a limit, half of them ignored it. Calculation of donor warm ischemia time (dWIT) varied substantially between countries. In ES and the UK, the starting point of dWIT was defined as deterioration of saturation/blood pressure, while in NL, cardiac arrest was used as starting point. Seventy‐eight percent of the respondents used a super‐rapid sterno‐laparotomy as procurement technique. Surgeons from NL and BE mainly used aortic perfusion (95% and 72%), while dual perfusion was more common in the UK (90%) and ES (91%).ConclusionsThis study demonstrates major differences in approach to DCD‐LT. To assure both donors and recipients a consistent standard of care, a consensus meeting on DCD‐LT is highly recommended.
Conjugacy classes, characters and products of elements
Authors: Guralnick Robert M. , Moretó Alexander 
Published: February 2019

AbstractRecently, Baumslag and Wiegold proved that a finite group G is nilpotent if and only if for every of coprime order. Motivated by this result, we study the groups with the property that and those with the property that for every and every nontrivial of pairwise coprime order. We also consider several ways of weakening the hypothesis on x and y. While the result of Baumslag and Wiegold is completely elementary, some of our arguments here depend on (parts of) the classification of finite simple groups.
A preliminary biopsychosocial analysis of online information on causes of neck pain
Authors: Neelapala Y.V. Raghava , Raja Riya , Bhandary Aishwarya 
Published: February 2019

AbstractObjectiveThe aim of the present study was to analyse the freely available online information on the causes of neck pain based on the biopsychosocial model of pain.MethodsA preliminary biopsychosocial analysis tool was developed, after an extensive literature review of the pathoanatomical and psychosocial contributors for neck pain. The websites that commonly appeared after the search term “causes of neck pain” in the first two pages of the search engines (Google, Yahoo and Bing) were selected for the biopsychosocial analysis. In addition, the websites were reviewed for Health on Net (HON) certification.ResultsTen websites were analysed, of which eight were identified to contain a predominant biomedical orientation, as they reported only the pathoanatomical causes of neck pain. The remaining two websites were determined to represent limited psychosocial information and described only two psychological contributors to the neck pain.ConclusionsThe online information on the causes of neck pain appears to contain limited biopsychosocial orientation. Further detailed analysis is essential to obtain firm conclusions on the content validity of online information on neck pain.