bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human adipose and umbilical cord mesenchymal stem cell-derived extracellular vesicles mitigate photoaging via TIMP1/Notch1
Authors: Zhang Huan , Xiao Xian , Wang Liping , Shi Xianhao , Fu Nan , Wang Shihua , Zhao Robert Chunhua 
Published: October 2024

AbstractUVB radiation induces oxidative stress, DNA damage, and inflammation, leading to skin wrinkling, compromised barrier function, and an increased risk of carcinogenesis. Addressing or preventing photoaging may offer a promising therapeutic avenue for these conditions. Recent research indicated that mesenchymal stem cells (MSCs) exhibit significant therapeutic potential for various skin diseases. Given that extracellular vesicles (EV) can deliver diverse cargo to recipient cells and elicit similar therapeutic effects, we investigated the roles and underlying mechanisms of both adipose-derived MSC-derived EV (AMSC-EV) and umbilical cord-derived MSC-derived EV (HUMSC-EV) in photoaging. Our findings indicated that in vivo, treatment with AMSC-EV and HUMSC-EV resulted in improvements in wrinkles and skin hydration while also mitigating skin inflammation and thickness alterations in both the epidermis and dermis. Additionally, in vitro studies using human keratinocytes (HaCaTs), human dermal fibroblast cells (HDFs), and T-Skin models revealed that AMSC-EV and HUMSC-EV attenuated senescence, reduced levels of reactive oxygen species (ROS) and DNA damage, and alleviated inflammation induced by UVB. Furthermore, EV treatment enhanced cell viability and migration capacity in the epidermis and promoted extracellular matrix (ECM) remodeling in the dermis in photoaged cell models. Mechanistically, proteomics results showed that TIMP1 was highly expressed in both AMSC-EV and HUMSC-EV and could exert similar effects as MSC-EV. In addition, we found that EV and TIMP1 could inhibit Notch1 and downstream targets Hes1, P16, P21, and P53. Collectively, our data suggests that both AMSC-EV and HUMSC-EV attenuate skin photoaging through TIMP1/Notch1.
Multisensory temporal processing in schizophrenia and bipolar disorder: implications for psychosis
Authors: Amadeo Maria Bianca , Escelsior Andrea , Esposito Davide , Inuggi Alberto , Versaggi Silvio , Marenco Giacomo , Massalha Yara , Bertolasi Jessica , Pereira da Silva Beatriz , Amore Mario , Serafini Gianluca , Gori Monica 
Published: October 2024

AbstractStructuring sensory events in time is essential for interacting with the environment and producing adaptive behaviors. Over the past years, the microstructure of temporality received increasing attention, recognized as a fundamental factor influencing cognitive, affective, and social abilities, whose alteration can underlie the etiopathogeneses of some clinical symptoms in psychiatric disorders. The present research investigated multisensory temporal processing in individuals with schizophrenia (N = 21), bipolar disorder (N = 20) and healthy controls (N = 21) in order to explore a plausible link between multisensory alterations in the temporal order of events and the psychopathological dimensions underlying psychosis. We asked participants to temporally order audio-tactile, visual-tactile, and audio-visual stimuli, and we administered different psychopathological scales to explore depressive, manic and psychotic symptoms. Results demonstrated that both subjects with schizophrenia and bipolar disorder are less precise in temporal order judgment independently of the sensory modalities involved. Interestingly, reduced precision in temporal processing of patients is positively associated with the presence and severity of positive symptoms. Our findings support the hypothesis that low-level sensory alterations in temporal structure may contribute to the emergence of clinical symptoms such as delusions, hallucinations, and disorganized behaviors.
Synthesis and characterization of new organometallic lanthanides metal complexes for photodynamic therapy
Authors: Fetouh H. A. , El-Mossalamy E. H. , El Desouky J. M. , Batouti Mervette El 
Published: October 2024

AbstractNew Schiff base ligand: 4-methoxy salicaldhyde-2-2-phenyl-hydrazono acetaldehíyde prepared by facile method. The molecular structures characterized by elemental analysis and proton magnetic resonance spectra (1H-NMR spectra). This spectra at the chemical shifts (3.5–10.39 ppm) confirmed the types and the numbers of protons. The sharp melting point at the range 110–112 °C confirmed purity. New optically active metal (samarium, terbium and gadolinium) complexes of the Schiff base synthesized in a one pot reaction. Vibrational IR spectra confirmed functional groups. Scanning electron microscopy micrographs confirmed that the modified microstructure of the metal complexes differed in morphology than the ligand. Powder X-ray diffraction patterns confirmed good crystalline structure. The optically activity of the solid metal complexes confirmed from electronic absorption spectra. The UV absorbance band at the wavelength range 280–390 nm and the intense phosphorescence bands up to 830 nm enabled application in photo dynamic therapy for apoptosis cancer cells by conversion triplet oxygen in the tissues into reactive singlet oxygen. Low charge transfer energy: 2.59–2.61 eV, high molar extinction coefficients (ε) at the order of magnitude $$\:{10}^{6}$$ M− 1 cm− 1 and the intense phosphorescence bands reflected good photodynamic activity. The metal complexes are thermally stable.
Structural dynamics of human deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase)
Authors: Sarre Ravdna , Dobrovolska Olena , Lundström Patrik , Turcu Diana , Agback Tatiana , Halskau Øyvind , Isaksson Johan 
Published: October 2024

AbstractStructural- and functional heterogeneity, as well as allosteric regulation, in homo-monomeric enzymes is a highly active area of research. One such enzyme is human nuclear-associated deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase), which has emerged as an interesting drug target in combination therapy with traditional nucleotide analogue treatment of cancer. We report, for the first time, a full structural dynamics study of human dUTPase by NMR. dUTPase has been investigated in terms of structural dynamics in its apo form, in complex with the modified substrate resistant to hydrolysis, 2’-deoxyuridine 5’-α,β-imido-triphosphate (dUpNHpp), as well as the product, 2’-deoxy-uridine-monophosphate (dUMP). The apo form of the enzyme displayed slow dynamics in the milli- to microsecond regime in relaxation dispersion experiments, which was further slowed down to observable heterogeneity upon substrate-analogue binding. The results suggest that the non-hydrolysable substrate-analogue traps the enzyme in the conformational isomerization step that has been previously suggested to be part of the enzyme catalysis kinetics cycle. The observed heterogeneity fits well with the pattern expected to emerge from the suggested kinetic model, and no evidence for homotropic allosterism was found. The heatmaps of the slow dynamics, chemical shift perturbation upon substrate binding and conserved regions of the enzyme sequence all displayed a similar pattern, which suggests that the structural dynamics is finely tuned and important for the biological function of the enzyme for binding, conformational shift, catalysis and substrate release.
Public attitudes towards social media field experiments
Authors: Straub Vincent J. , Burton Jason W. , Geers Michael , Lorenz-Spreen Philipp 
Published: October 2024

Abstract
The growing use of social media field experiments demands a rethink of current research ethics in computational social science and psychological research. Here, we provide an exploratory empirical account of key user concerns and outline a number of critical discussions that need to take place to protect participants and help researchers to make use of the novel opportunities of digital data collection and field studies. Our primary contention is that we need to elicit public perceptions to devise more up-to-date guidelines for review boards whilst also allowing and encouraging researchers to arrive at more ethical individual study design choices themselves. To ground our discussion in real-world examples of online experiments, we focus on recent social media studies in the field of misinformation, polarization, and hate speech research. We conclude by discussing how we can better strike a balance between meeting ethical guidelines and the concerns of social media users alongside maximizing scientific impact and credibility.
Liver magnetic resonance imaging, non-alcoholic fatty liver disease and metabolic syndrome risk in pre-pubertal Mexican boys
Authors: de Celis Alonso Benito , Shumbayawonda Elizabeth , Beyer Cayden , Hidalgo-Tobon Silvia , López-Martínez Briseida , Dies-Suarez Pilar , Klunder-Klunder Miguel , Miranda-Lora América Liliana , Pérez Eduardo Barragán , Thomaides-Brears Helena , Banerjee Rajarshi , Thomas E. Louise , Bell Jimmy D. , So Po-Wah 
Published: October 2024

AbstractRising global pediatric obesity rates, increase non-alcoholic fatty liver disease (NAFLD) and metabolic syndrome (MetS) prevalence, with MetS being a NAFLD risk factor. NAFLD can be asymptomatic, with liver function tests insensitive to mild disease, and liver biopsy, risking complications. Thus, we investigated multiparametric MRI (mpMRI) metrics of liver fat (proton density fat fraction, PDFF) and disease activity (fibro-inflammation; iron-corrected T1, cT1), in a Hispanic pre-pubertal pediatric cohort, with increased risk of NAFLD. Pre-pubertal boys (n = 81) of varying Body-Mass Index (BMI) were recruited in Mexico City. Most children (81%) had normal liver transaminase levels, 38% had high BMI, and 14% had ≥ 3 MetS risk factors. Applying mpMRI thresholds, 12%, 7% and 4% of the cohort had NAFLD, NASH and high-risk NASH respectively. Participants with ≥ 3 MetS risk factors had higher cT1 (834 ms vs. 737 ms, p = 0.004) and PDFF (8.7% vs. 2.2%, p < 0.001) compared to those without risk factors. Those with elevated cT1 tended to have high BMI and high insulin (p = 0.005), HOMA-IR (p = 0.005) and leptin (p < 0.001). The significant association of increased risk of MetS with abnormal mpMRI, particularly cT1, proposes the potential of using mpMRI for routine pediatric NAFLD screening of high-risk (high BMI, high MetS risk score) populations.
Synthesis and characterization of MoS2-carbon based materials for enhanced energy storage applications
Authors: Szkoda Mariusz , Ilnicka Anna , Trzciński Konrad , Zarach Zuzanna , Roda Daria , Nowak Andrzej P. 
Published: October 2024

AbstractThe article delves into the synthesis and characterization of MoS2-carbon-based materials, holding promise for applications in supercapacitors and ion batteries. The synthesis process entails the preparation of MoS2 and its carbon hybrids through exfoliation, hydrothermal treatment, and subsequent pyrolysis. Various analytical techniques were employed to comprehensively examine the structural, compositional, and morphological properties of the resulting materials. The article explores the electrochemical performance of these electrode materials in supercapacitors and ion batteries (LiB, SiB, KiB). Electrochemical measurements were conducted in aqueous electrolyte for supercapacitors and various aprotic electrolytes for ion batteries. Results highlight the impact of the synthesis process on electrochemical performance, emphasizing factors such as capacitance, rate capability, and charge/discharge cycle performance. Hydrothermally treated MoS2-carbon exhibited a specific capacitance of approximately 150 F g-1 in supercapacitors, attributed to its high surface area and efficient charge storage mechanisms. Additionally, for Li-ion battery materials without hydrothermal treatment showed impressive capacity retention of around 88% after 500 charge-discharge cycles, starting with an initial specific capacity of about 920 mAh/g. Long-term stability was demonstrated in both supercapacitors and lithium-ion batteries, with minimal capacitance degradation even after extensive charge-discharge cycles. This research underscores the potential of MoS2-based materials as effective energy storage solutions.
The predictability of fluctuating environments shapes the thermal tolerance of marine ectotherms and compensates narrow safety margins
Authors: Fusi Marco , Barausse Alberto , Booth Jenny Marie , Chapman Erica , Daffonchio Daniele , Sanderson William , Diele Karen , Giomi Folco 
Published: October 2024

AbstractAquatic species living in productive coastal habitats with abundant primary producers have evolved in highly dynamic diel and seasonally fluctuating environments in terms of, for example, water temperature and dissolved oxygen. However, how environmental fluctuations shape the thermal tolerance of marine species is still poorly understood. Here we hypothesize that the degree of predictability of the diel environmental fluctuations in the coastal area can explain the thermal response of marine species. To test this hypothesis, we measured the thermal tolerance of 17 species of marine ectotherm from tropical, warm temperate and cold temperate latitudes under two levels of oxygen (around saturation and at supersaturation), and relate the results to their site-specific temperature and oxygen fluctuation and their environmental predictability. We demonstrate that oxygen and temperature fluctuations at tropical latitudes have a higher predictability than those at warm and cold temperate latitudes. Further, we show that marine species that are adapted to high predictability have the potential to tune their thermal performance when exposed to oxygen supersaturation, despite being constrained within a narrow safety margin. We advocate that the predictability of the environmental fluctuation needs to be considered when measuring and forecasting the response of marine animals to global warming.
Polyethyleneimine facilitates the growth and electrophysiological characterization of iPSC-derived motor neurons
Authors: Yang Meimei , You Daofeng , Liu Gang , Lu Yin , Yang Guangming , O’Brien Timothy , Henshall David C. , Hardiman Orla , Cai Li , Liu Min , Shen Sanbing 
Published: October 2024

AbstractInduced pluripotent stem cell (iPSC) technology, in combination with electrophysiological characterization via multielectrode array (MEA), has facilitated the utilization of iPSC-derived motor neurons (iPSC-MNs) as highly valuable models for underpinning pathogenic mechanisms and developing novel therapeutic interventions for motor neuron diseases (MNDs). However, the challenge of MN adherence to the MEA plate and the heterogeneity presented in iPSC-derived cultures raise concerns about the reproducibility of the findings obtained from these cellular models. We discovered that one novel factor modulating the electrophysiological activity of iPSC-MNs is the extracellular matrix (ECM) used in the coating to support in vitro growth, differentiation and maturation of iPSC-MNs. The current study showed that two coating conditions, namely, Poly-L-ornithine/Matrigel (POM) and Polyethyleneimine (PEI) strongly promoted attachment of iPSC-MNs on MEA culture dishes compared to three other coating conditions, and both facilitated the maturation of iPSC-MNs as characterized by the detection of extensive electrophysiological activities from the MEA plates. POM coating accelerated the maturation of the iPSC-MNs for up to 5 weeks, which suits modeling of neurodevelopmental disorders. However, the application of PEI resulted in more even distribution of the MNs on the culture dish and reduced variability of electrophysiological signals from the iPSC-MNs in 7-week cultures, which permitted the detection of enhanced excitability in iPSC-MNs from patients with amyotrophic lateral sclerosis (ALS). This study provides a comprehensive comparison of five coating conditions and offers POM and PEI as favorable coatings for in vitro modeling of neurodevelopmental and neurodegenerative disorders, respectively.
Consensus tissue domain detection in spatial omics data using multiplex image labeling with regional morphology (MILWRM)
Authors: Kaur Harsimran , Heiser Cody N. , McKinley Eliot T. , Ventura-Antunes Lissa , Harris Coleman R. , Roland Joseph T. , Farrow Melissa A. , Selden Hilary J. , Pingry Ellie L. , Moore John F. , Ehrlich Lauren I. R. , Shrubsole Martha J. , Spraggins Jeffrey M. , Coffey Robert J. , Lau Ken S. , Vandekar Simon N. 
Published: October 2024

AbstractSpatially resolved molecular assays provide high dimensional genetic, transcriptomic, proteomic, and epigenetic information in situ and at various resolutions. Pairing these data across modalities with histological features enables powerful studies of tissue pathology in the context of an intact microenvironment and tissue structure. Increasing dimensions across molecular analytes and samples require new data science approaches to functionally annotate spatially resolved molecular data. A specific challenge is data-driven cross-sample domain detection that allows for analysis within and between consensus tissue compartments across high volumes of multiplex datasets stemming from tissue atlasing efforts. Here, we present MILWRM (multiplex image labeling with regional morphology)—a Python package for rapid, multi-scale tissue domain detection and annotation at the image- or spot-level. We demonstrate MILWRM’s utility in identifying histologically distinct compartments in human colonic polyps, lymph nodes, mouse kidney, and mouse brain slices through spatially-informed clustering in two different spatial data modalities from different platforms. We used tissue domains detected in human colonic polyps to elucidate the molecular distinction between polyp subtypes, and explored the ability of MILWRM to identify anatomical regions of the brain tissue and their respective distinct molecular profiles.
ZC3HAV1 facilitates STING activation and enhances inflammation
Authors: Qin Danhui , Song Hui , Wang Caiwei , Ma Xiaojie , Fu Yu , Zhao Chunyuan , Zhao Wei , Zhang Lei , Zhang Weifang 
Published: October 2024

AbstractStimulator of interferon genes (STING) is vital in the cytosolic DNA-sensing process and critical for initiating the innate immune response, which has important functions in host defense and contributes to the pathogenesis of inflammatory diseases. Zinc finger CCCH-type antiviral protein 1 (ZC3HAV1) specifically binds the CpG dinucleotides in the viral RNAs of multiple viruses and promotes their degradation. ZAPS (ZC3HAV1 short isoform) is a potent stimulator of retinoid acid-inducible gene I (RIG-I) signaling during the antiviral response. However, how ZC3HAV1 controls STING signaling is unclear. Here, we show that ZC3HAV1 specifically potentiates STING activation by associating with STING to promote its oligomerization and translocation from the endoplasmic reticulum (ER) to the Golgi, which facilitates activation of IRF3 and NF-κB pathway. Accordingly, Zc3hav1 deficiency protects mice against herpes simplex virus-1 (HSV-1) infection- or 5,6-dimethylxanthenone-4-acetic acid (DMXAA)-induced inflammation in a STING-dependent manner. These results indicate that ZC3HAV1 is a key regulator of STING signaling, which suggests its possible use as a therapeutic target for STING-dependent inflammation.
Beyond blooms: the winter ecosystem reset determines microeukaryotic community dynamics in the Fram Strait
Authors: Oldenburg Ellen , Kronberg Raphael M. , Metfies Katja , Wietz Matthias , von Appen Wilken-Jon , Bienhold Christina , Popa Ovidiu , Ebenhöh Oliver 
Published: October 2024

AbstractThe Arctic Ocean is undergoing a major transition as a result of global warming, with uncertain consequences for its ecosystems. Our study introduces an integrated analytical approach using co-occurrence networks, convergent cross-mapping, and energy landscape analysis. Applied to four years of amplicon data from Fram Strait, located at the boundary between Arctic and Atlantic waters, our method identifies keystone species in seasonal microbial communities, elucidates causal interactions, and predicts stable configurations across changing environments. We find strong evidence for a “winter reset”, implying that organisms representing the spring bloom are largely determined by prevailing environmental conditions during winter. In addition, our analysis suggests that winter communities may adapt more readily to expected Atlantification than summer communities. These results highlight the utility of innovative time-series analyses in disentangling ecosystem dynamics. This approach provides critical insights into Arctic ecological interactions, dynamics, and resilience and aids in understanding ecosystem responses to environmental change.
An Evaluation of the Psychometric Properties of the Short Form of the Speech, Spatial and Qualities of Hearing Scale in Swedish: Online Versus Paper-and-Pen
Authors: Ahlberg Sandra , Brännström Jonas , Öberg Marie , Thorén Elisabet 
Published: October 2024

Purpose:
The purpose of this study was to examine the psychometric properties of the Swedish short form of the Speech, Spatial and Qualities of Hearing Scale (SSQ12) and investigate whether the paper-and-pen and online formats could be used interchangeably.

Method:

Individuals with and without hearing problems were invited to participate in this study. The participants (
N
= 125) were randomized into four groups: paper–paper, online–online, paper–online, and online–paper. All participants completed the Swedish SSQ12 twice.

Results:
Principal components analysis revealed one component. Statistical analysis revealed good psychometric properties. Administration formats were compared using repeated-measures analysis of variance, which revealed no statistically significant differences.

Conclusions:
The results indicate that the Swedish SSQ12 is possible to use in paper-and-pen and online formats interchangeably. The questionnaire has potential to be used by Swedish audiologists seeking to understand the individual experience of hearing loss or to evaluate hearing rehabilitation. To further understand the possible differences and to broaden the use and understanding of the SSQ12, future studies should aim to determine the minimal clinically important difference for the SSQ12.