bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative prognostic subtype discovery in high‐grade serous ovarian cancer
Authors: Xie Hongyu , Xu Huan , Hou Yan , Cai Yuqing , Rong Zhiwei , Song Wei , Wang Wenjie , Li Kang 
Published: July 2019

AbstractObjectiveWe sought to identify novel molecular subtypes of high‐grade serous ovarian cancer (HGSC) by the integration of gene expression and proteomics data and to find the underlying biological characteristics of ovarian cancer to improve the clinical outcome.MethodsThe iCluster method was utilized to analysis 131 common HGSC samples between TCGA and Clinical Proteomic Tumor Analysis Consortium databases. Kaplan‐Meier survival curves were used to estimate the overall survival of patients, and the differences in survival curves were assessed using the log‐rank test.ResultsTwo novel ovarian cancer subtypes with different overall survival (P = .00114) and different platinum status (P = .0061) were identified. Eighteen messenger RNAs and 38 proteins were selected as differential molecules between subtypes. Pathway analysis demonstrated arrhythmogenic right ventricular cardiomyopathy pathway played a critical role in the discrimination of these two subtypes and desmosomal cadherin DSG2, DSP, JUP, and PKP2 in this pathway were overexpression in subtype I compared with subtype II.ConclusionOur study extended the underlying prognosis‐related biological characteristics of high‐grade serous ovarian cancer. Enrichment of desmosomal cadherin increased the risk for HGSC prognosis among platinum‐sensitive patients, the results guided the revision of the treatment options for platinum‐sensitive ovarian cancer patients to improve outcomes.
New strategy of bone marrow mesenchymal stem cells against oxidative stress injury via Nrf2 pathway: oxidative stress preconditioning
Authors: Zhang Fei , Peng Wuxun , Zhang Jian , Dong Wentao , Yuan Dajiang , Zheng Yinggang , Wang Zhenwen 
Published: July 2019

AbstractClinically, bone marrow mesenchymal stem cells (BMSCs) have been used in treatment of many diseases, but the local oxidative stress (OS) of lesion severely limits the survival of BMSCs, which reduces the efficacy of BMSCs transplantation. Therefore, enhancing the anti‐OS stress ability of BMSCs is a key breakthrough point. Preconditioning is a common protective mechanism for cells or body. Here, the aim of this study was to investigate the effects of OS preconditioning on the anti‐OS ability of BMSCs and its mechanism. Fortunately, OS preconditioning can increase the expression of superoxide dismutase, catalase, NQO1, and heme oxygenase 1 through the nuclear factor erythroid 2‐related factor 2 pathway, thereby decreased the intracellular reactive oxygen species (ROS) levels, relieved the damage of ROS to mitochondria, DNA and cell membrane, enhanced the anti‐OS ability of BMSCs, and promoted the survival of BMSCs under OS.
Intrapersonal and interpersonal facilitators of forgiveness following spousal infidelity: A stress and coping perspective
Authors: Chi Peilian , Tang Yixin , Worthington Everett L. , Chan Cecilia L. W. , Lam Debbie O. B. , Lin Xiuyun 
Published: July 2019

AbstractObjectiveForgiveness includes processes that involve a decision to stop bitterness and thoughts of revenge (i.e., decisional forgiveness), which further motivates the forgiver towards the restoration of positive emotions (i.e., emotional forgiveness). Using stress and coping framework, this study investigated intrapersonal and interpersonal facilitators of decisional and emotional forgiveness in a Chinese marital context.MethodParticipants were 154 respondents who had experienced or were experiencing spousal infidelity.ResultsSolidarity‐oriented personality and perceived partner's reconciliation motivation facilitated benign attributions and empathy, then facilitated higher levels of decisional forgiveness, which promoted emotional forgiveness. Strength of marital bond before the infidelity directly predicted higher levels of emotional forgiveness.ConclusionsOur findings provide evidence for the differentiated decisional and emotional forgiveness processes after spousal infidelity and delineate different coping mechanism that triggers them, thus lending culturally appropriate evidence for clinicians who work with clients facing spousal infidelity.
Long noncoding RNA NEAT1 sponges miR‐495‐3p to enhance myocardial ischemia‐reperfusion injury via MAPK6 activation
Authors: Luo Man , Sun Qingsong , Zhao Hongmei , Tao Jiali , Yan Dongsheng 
Published: July 2019

AbstractThe biological function of long noncoding RNA NEAT1 has been revealed in a lot of diseases. Nevertheless, it is still not yet clear whether NEAT1 can modulate the process of myocardial ischemia–reperfusion injury (M‐I/R). Here, we reported that NEAT1 was able to sponge miR‐495‐3p to contribute to M‐I/R injury through activating mitogen‐activated protein kinase 6 (MAPK6). First, elevated expression of NEAT1 was revealed in M‐I/R injury mice, meanwhile, lactate dehydrogenase (LDH) and creatine kinase‐muscle/brain (CK‐MB) were also upregulated in the serum. Meanwhile, as previously reported, miR‐495 serves as a tumor suppressor or an oncogenic miRNA in different types of cancer. Currently, we found miR‐495‐3p was remarkably reduced in M‐I/R mice. Additionally, NEAT1 was significantly induced whereas miR‐495‐3p was greatly reduced by H2O2 treatment in H9C2 cells. Moreover, loss of NEAT1 in H9C2 cells could repress the viability and proliferation of cells. For another, overexpression of NEAT1 exhibited an opposite phenomenon. Furthermore, LDH release and caspase‐3 activity were obviously triggered by upregulation of NEAT1 while suppressed by NEAT1 knockdown. miR‐495‐3p was indicated and validated as a target of NEAT1 using the analysis of bioinformatics. Interestingly, we observed that miR‐495‐3p mimics repressed tumor necrosis factor‐α (TNF‐α), interleukin‐1β (IL‐1β), and IL‐18 protein expression while their levels were enhanced by the inhibition of miR‐495‐3p in H9c2 cells. Subsequently, it was manifested that MAPK6 was a target of miR‐495‐3p, which could exert a lot in the NEAT1/miR‐495‐3p‐mediated M‐I/R injury. Overall, our results implied that NEAT1 contributed to M‐I/R injury via the modulation of miR‐495‐3p and MAPK6.
RETRACTED: Long noncoding RNA WDFY3‐AS2 suppresses tumor progression by acting as a competing endogenous RNA of microRNA‐18a in ovarian cancer
Authors: Li Weijia , Ma Shihong , Bai Xiaoxu , Pan Wenjing , Ai Limin , Tan Wenhua 
Published: July 2019

AbstractOvarian cancer (OC) is a fatal cancer in women, mainly due to its aggressive nature and poor survival rate. The lncRNA‐miRNA‐mRNA (long noncoding RNA‐microRNA‐messenger RNA) interaction is promising biomarkers for the improving prognosis of OC. Therefore, we explored the regulatory mechanism of WDFY3‐AS2/miR‐18a/RORA axis involved in the biological activities of OC cells. Microarray analysis predicted differentially expressed lncRNA, miRNA, and mRNA related to OC, followed by investigating the relationship among them. The expression patterns of the identified lncRNA WDFY3‐AS2, miR‐18a, and RORA were measured in OC tissue and cells. Gain‐ and loss‐of‐function experiments were performed to characterize the effect of lncRNA WDFY3‐AS2 on OC cells, as well as the involvement of miR‐18a and RAR related orphan receptor A (RORA). The in vitro assays were validated by in vivo experiments. According to bioinformatics analysis, WDFY3‐AS2 was speculated to affect OC by sponging miR‐18a and modulating RORA. WDFY3‐AS2 and RORA were underexpressed in OC, while miR‐18a was highly expressed. Notably, WDFY3‐AS2 acts as a competing endogenous RNA to sponge miR‐18a and upregulate RORA. Upon overexpressing WDFY3‐AS2 or inhibiting miR‐18a, RORA expression was increased, thereby the OC cell proliferation, migration, invasion, and epithelial‐to‐mesenchymal transition (EMT) were suppressed, accompanied by enhanced apoptosis. In vivo experiments confirmed that the tumor growth was reduced in response to overexpressed WDFY3‐AS2 or inhibited miR‐18a. Taken together, the lncRNA WDFY3‐AS2/miR‐18a axis regulates the tumor progression of OC by targeting RORA, providing new insights for prevention and control of OC.
Glycochenodeoxycholate promotes the metastasis of gallbladder cancer cells by inducing epithelial to mesenchymal transition via activation of SOCS3/JAK2/STAT3 signaling pathway
Authors: Wang Hui , Zhan Ming , Liu Qiang , Wang Jian 
Published: July 2019

AbstractThe incidence of gallbladder cancer (GBC) is relatively rare but a high degree of malignancy. The migration and invasion potential of GBC severely affects the prognosis of patients with GBC. Glycochenodeoxycholate (GCDC) is one of the most important components in GBC‐associated microenvironment. However, the role of GCDC in the metastatic feature of GBC cells is not fully understood. First, the results of this study found that GCDC could effectively enhance the metastasis of GBC cells. Furthermore, GCDC could lead to the enhancement of epithelial to mesenchymal transition (EMT) phenotype in GBC cells, which is concerned to be an important mechanism of tumor metastasis. Further studies showed that GCDC treatment induced the upregulation of matrix metalloproteinase‐3 (MMP3), MMP9, and SOCS3/JAK2/p‐STAT3 signal pathway in GBC cells, which could regulate the level of EMT. Beside that, we also found the positive expression of farnesoid X receptor (FXR) in GBC cells and inhibition of FXR could significantly block the effect of GCDC on the metastasis of GBC cells. These results indicated that GCDC promoted GBC cells metastasis by enhancing the level of EMT and inhibition of FXR could significantly block the effect of GCDC. On one hand, FXR might be an indicator for predicting the metastasis of patient with GBC. On the other hand, FXR might serve as a potential antimetastasis target in GBC therapy.
Highly multiplex guide RNA expression units of CRISPR/Cas9 were completely stable using cosmid amplification in a novel polygonal structure
Authors: Nakanishi Tomoko , Maekawa Aya , Tabata Hirotaka , Yoshioka Takashi , Pei Zheng , Sato Kumiko , Mori Mai , Kato Masaya , Saito Izumu 
Published: July 2019

AbstractBackgroundGenome editing using the CRISPR/Cas9 system is now well documented in basic studies and is expected to be applied to gene therapy. Simultaneous expression of multiplex guide RNA (gRNA) and Cas9/Cas9 derivative is attractive for the efficient knockout of genes and a safe double‐nicking strategy. However, such use is limited because highly multiplex gRNA‐expressing units are difficult to maintain stably in plasmids as a result of deletion via homologous recombination.MethodsLambda in vitro packaging was used instead of transformation for the construction and preparation of large, cos‐containing plasmid (cosmid). Polymerase chain reaction fragments containing multiplex gRNA units were obtained using the Four‐guide Tandem method. Transfection was performed by lipofection.ResultsWe constructed novel cosmids consisting of linearized plasmid‐DNA fragments containing up to 16 copies of multiplex gRNA‐expressing units as trimer or tetramer (polygonal cosmids). These cosmids behaved as if they were monomer plasmids, and multiplex units could stably be maintained and amplified with a lack of deletion. Surprisingly, the deleted cosmid was removed out simply by amplifying the cosmid stock using lambda packaging. The DNA fragments containing multiplex gRNA‐units and Cas9 were transfected to 293 cells and were found to disrupt the X gene of hepatitis B virus by deleting a large region between the predicted sites.ConclusionsWe present a simple method for overcoming the problem of constructing plasmids stably containing multiplex gRNA‐expressing units. The method may enable the production of very large amounts of DNA fragments expressing intact, highly‐multiplex gRNAs and Cas9/Cas9 derivatives for safe and efficient genome‐editing therapy using non‐viral vectors.
Using a HIV registry to develop accurate estimates for the HIV care cascade – the Singapore experience
Authors: Ho Zheng Jie Marc , Huang Flora , Wong Chen Seong , Chua Lily , Ma Stefan , Chen Mark I‐Cheng , Lee Vernon J 
Published: July 2019

AbstractIntroductionAchieving UNAIDS 90‐90‐90 targets is a crucial step towards ending the AIDS epidemic. Many countries have published estimates of care cascades, although often with methodological limitations. We describe an approach that used the national HIV registry as a starting‐point to determine the HIV care cascade and resulting UNAIDS 90‐90‐90 estimates for Singapore in 2014.MethodsHIV is a legally notifiable disease in Singapore. The anonymized HIV registry data provided for a back‐calculation model from the European Centre for Disease Prevention and Control to obtain 2014 estimates for the total number of persons living with HIV (PLHIV), and the count in the registry for proportions diagnosed with HIV and linked to care. Using additional data collected for a simple random sample from the registry, outcomes in 2015 and 2016 were ascertained retrospectively to derive proportions for those retained in care, on antiretroviral therapy, and achieved viral suppression. Findings were extrapolated to derive national estimates and UN90‐90‐90 estimates. Bootstrapped samples from the model and sample were used to derive 95% confidence intervals.ResultsAn estimated 6900 (95% CI 6650, 7050) persons were living with HIV and AIDS in 2014. Of these, 4948 were diagnosed with HIV, and 4820 had been linked to care. The random sample of 500 persons was further analysed, and of these, 87.2% were retained in care, 84.6% on antiretroviral therapy, and 79.6% had suppressed viral loads. The proportions of HIV‐infected individuals on antiretroviral therapy and achieving viral suppression were 60.7% (95% CI 58.4, 63.6) and 57.1% (95% CI 55.0, 60.5) respectively. The corresponding UNAIDS 90‐90‐90 estimates were 71.7% (95% CI 70.0, 74.2) of all persons diagnosed; 84.6% (95% CI 81.6, 87.4) of diagnosed persons being on antiretroviral therapy, and 94.1% (95% CI 91.6, 96.2) of persons on therapy having achieved viral suppression.ConclusionsA national HIV registry, alongside back‐calculation and additional data from a sample, can be used to estimate attainment of UNAIDS 90‐90‐90 targets and identify system gaps. The registry had advantages of providing a well‐established, comprehensive capture of diagnosed persons and easily accessible data. The same approach can be used elsewhere if similar data are available.
Artificially designed hepatitis B virus core particles composed of multiple epitopes of type A and O foot‐and‐mouth disease virus as a bivalent vaccine candidate
Authors: Lei Yao , Shao Junjun , Zhao Furong , Li Yangfan , Lei Chenglin , Ma Feifei , Chang Huiyun , Zhang Yongguang 
Published: July 2019

AbstractRecently, many countries, including China, have experienced a series of type A and O foot‐and‐mouth disease virus (FMDV) epidemics, causing serious economic losses. Although concerns about the safety of inactivated FMD vaccines have been raised, the development of a safe and effective subunit vaccine is necessary. We constructed two chimeric virus‐like particles (VLPs; rHBc/AO and rHBc/AOT VLPs) displaying tandem repeats of B cell epitopes (VP1 residue 134‐161 and 200‐213) derived from type A and O FMDV and one T cell epitope (3 A residue 21‐35) using the truncated hepatitis B virus core (HBc) carrier. Our results indicate that the chimeric HBc can self‐assemble into VLPs with these FMDV epitopes displayed on the surface. Immunization with the chimeric VLPs induced specific IgG and neutralization antibodies against type A and O FMDV in mice. Compared with the commercial type A/O FMDV bivalent inactivated vaccine, rHBc/AO and rHBc/AOT VLPs significantly stimulated the production of Th1 type cytokines (IFN‐γ and IL‐2), whereas Th2 cytokine production (IL‐4 and IL‐10) was decreased. Compared with rHBc/AO, rHBc/AOT induced increased Th2 cytokine and specific IgG production. These results demonstrate that the VLPs constructed in the current study induced both humoral and cellular immune responses and may represent potential bivalent VLP vaccines targeting both FMDV type A and O strains.
An optimal far‐field radiation pattern synthesis of time‐modulated linear and concentric circular antenna array
Authors: Das Avishek , Mandal Durbadal , Kar Rajib 
Published: July 2019

AbstractIn this paper, an optimal far‐field radiation pattern synthesis by using time modulation technique is carried out for linear antenna array (LAA), and concentric circular antenna array (CCAA) designs using a stochastic optimization method called grey wolf optimization (GWO). An improved far‐field radiation characteristic mainly depends on the synthesis of low side lobe level (SLL) and narrow first null beam width (FNBW). A low SLL is a requirement to reduce the interference by the other systems operating in the same frequency band. A narrow FNBW is necessary to obtain a high directivity. In time modulation technique, “time” has a most significant role. It is considered as the fourth‐dimensional parameter as it plays the role of an additional degree of freedom to achieve an improved radiation pattern. The time‐modulated antenna array (TMAA) radiates at various harmonic frequencies for the periodic switching time sequence. In this paper, the generation of radiation pattern only at the fundamental/central frequency and at its first two harmonic frequencies are considered. The GWO algorithm is a flexible and robust search algorithm and is applied here to find out the optimum sets of switching on‐time duration for each array element and to determine the optimum inter‐element separation between two array elements of the 16‐element time‐modulated linear antenna array (TMLAA) and the nine‐ring time‐modulated concentric circular antenna array (TMCCAA). The GWO algorithm‐based results show a substantial improvement in far‐field radiation pattern synthesis compared with the uniform radiation pattern and those of the methods reported in state‐of‐the‐art literature.
Effect of biosurfactant derived from Vibrio natriegens MK3 against Vibrio harveyi biofilm and virulence
Authors: Kannan Suganya , Krishnamoorthy Govindan , Kulanthaiyesu Arunkumar , Marudhamuthu Murugan 
Published: July 2019

AbstractVibrio harveyi is a marine luminous pathogen, which causes biofilm‐mediated infections, pressures the search for an innovative alternate approach to strive against vibriosis in aquaculture. This study anticipated to explore the effect of glycolipid biosurfactant as an antipathogenic against
V. harveyi to control vibriosis. In this study, 27 bacterial strains were isolated from marine soil sediments. Out of these, 11 strains exhibited surfactant activity and the strain MK3 showed high emulsification index. The potent strain was identified as
Vibrio natriegens and named as
V. natriegens MK3. The extracted biosurfactant was purified using high‐performance liquid chromatography and it was efficient to decrease the surface tension of the growth medium up to 21 mN/m. The functional group and composition of the biosurfactant were determined by Fourier‐transform infrared spectroscopy and nuclear magnetic resonance spectroscopy spectral studies and the nature of the biosurfactant was identified as glycolipid. The surfactant was capable of reducing the biofilm formation, bioluminescence, extracellular polysaccharide synthesis, and quorum sensing in marine shrimp pathogen
V. harveyi. The antagonistic effect of biosurfactant was evaluated against
V. harveyi‐infected brine shrimp
Artemia salina. This study reveals that biosurfactant can be considered for the management of biofilm‐related aquatic infections.
Analysis of the structures of confirmed and questionable CRISPR loci in 325 Staphylococcus genomes
Authors: Zhang Mengmeng , Bi Chunxia , Wang Mengyuan , Fu Hengxia , Mu Zhengrong , Zhu Yuanqi , Yan Zhiyong 
Published: July 2019

AbstractThe CRISPR‐Cas (clustered regular interspaced short palindromic repeats and CRISPR‐associated proteins) system is a newly discovered immune defense system in the genome of prokaryotes, which can resist the invasion of foreign genetic elements, such as plasmids or phage. In this study, 154 strains of Staphylococcus published in the CRISPRDatabase and 171 strains included in NCBI were downloaded, the confirmed and questionable CRISPR loci of which were analyzed by bioinformatics methods, including their distribution, characteristics of the structure (including the direct repeats, spacers and cas genes), and the relationship between the presence of CRISPR and the mecA gene. Meanwhile, a comprehensive analysis of orphan CRISPR arrays was performed on this basis. A total of 196 confirmed and 1757 questionable CRISPR loci were found in 325 Staphylococcus genomes. Only 25 strains contained cas genes, which were classified into III‐A (48.1%) and II‐C (51.9%). The difference between the presence of the cas gene and the carrying rate of mecA was statistically significant, and they were negatively correlated. A total of 137 confirmed and 1755 questionable CRISPR loci were assumed to be false‐CRISPR. The present study also analyzed the questionable CRISPR array for the first time while analyzing the confirmed CRISPR array in the Staphylococcal genome and screened the false‐CRISPR elements in the orphan CRISPR array.
Enhanced conversion of sterols to steroid synthons by augmenting the peptidoglycan synthesis gene pbpB in Mycobacterium neoaurum
Authors: Sun Wan‐Ju , Liu Yong‐Jun , Liu Hao‐Hao , Ma Jie‐De , Ren Yu‐Hong , Wang Feng‐Qing , Wei Dong‐Zhi 
Published: July 2019

AbstractSome species of mycobacteria have been modified to transform sterols to valuable steroid synthons. The unique cell wall of mycobacteria has been recognized as an important organelle to absorb sterols. Some cell wall inhibitors (e.g., vancomycin and glycine) have been validated to enhance sterol conversion by interfering with transpeptidation in peptidoglycan biosynthesis. Therefore, two transpeptidase genes, pbpA and pbpB, were selected to rationally modify the cell wall to simulate the enhancement effect of vancomycin and glycine on sterol conversion in a 22‐hydroxy‐23,24‐bisnorchol‐4‐ene‐3‐one (4‐HBC) producing strain (WIII). Unexpectedly, the pbpA or pbpB gene augmentation was conducive to the utilization of sterols. The pbpB augmentation strain WIII‐pbpB was further investigated for its better performance. Compared to WIII, the morphology of WIII‐pbpB was markedly changed from oval to spindle, indicating alterations of the cell wall. Biochemical analysis indicated that the altered cell wall properties of WIII‐pbpB might contribute to the positive effect on sterol utilization. The productivity of 4‐HBC was enhanced by 28% in the WIII‐pbpB strain compared to that of WIII. These results demonstrated that the modification of peptidoglycan synthesis can improve the conversion of sterols to steroid synthons in mycobacteria.