bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical and Endoscopic Presentation of Primary Gastric Lymphoma: An Italian Multicenter Study.
Authors: Patti Caterina , Zullo A. , Tedeschi L. , Di Raimondo F. , Hassan C. , Recine U. , Bibas M. , Miedico A. , Romanelli A. , Vinci M. , Costa A. , Restivo G. , Mistretta A. , Montalbano L. , Linea C. , Marrone C. , Caruso L. , Chiarenza A. , Bonanno G. , Lizzani G. , Stella F. , Mirto S. , Morini S. , Cottone M. , Andriani Alessandro 
Published: October 2019

Abstract
Background. The stomach is the most frequent site of intestinal lymphomas. However, few data are available on the clinical-endoscopic presentation of gastric lymphoma as well as on possible differences in clinical pattern and endoscopic features between low-grade (LG) and high-grade (HG) lymphomas. In this study, we evaluated such aspects on consecutive primary gastric lymphoma patients observed in the last 12 years (1993–2004) in four Italian Hospitals (1 North, 2 Centre, 2 South).
Methods. Clinical, histological, and endocospic records of consecutive patients diagnosed with LG or HG gastric lymphoma were retrieved and accurately evaluated. Symptoms were categorized as “alarm” (anaemia/melaena/heamorrage, persistent vomiting, weight loss) or “no alarm” (epigastric/abdominal pain, heartburn, dyspepsia/bloating). The endoscopic findings were classified as “normal” (no macroscopic lesions) or “abnormal” (ulcer, erosions, nodular pattern, hypertrophic folds, polypoid mass). Statistical analysis was carried out by using the Chi squared test.
Results. During the study period, 143 patients with primary gastric lymphoma were detected. Overall, 61 patients were observed in the first 6 years and 82 in the last 6 years. The main results of the study are summarized in the table 1.
Conclusions. The incidence of primary gastric lymphoma seems to be increasing. The overall prevalence of alarm symptoms is quite low, and they may be absent in near 75% of LG lymphoma patients. Moreover, contrarily to HG, LG lymphoma may present as a normal endoscopic finding and it is more frequently associated with H. pylori infection. At diagnosis, HG lymphoma is more frequently detected in an advanced stage as compared to LG lymphoma.
Overall (143 patients) LG lymphoma (73 patients) HG lymphoma (70 patients) P value Age (mean ± SD) yrs 59.5 ± 14.2 59.4 ± 13.3 59.7 ± 15.1 0.4 Sex (M /F) 83/60 44/29 39/31 0.6 Alarm symptoms 57 (40%) 19 (26%) 38 (54%) 0.0009 Normal endoscopy 15 (10%) 15 (20%) 0 (0%) 0.0004 H. pylori infection 66 (73%) 47 (86%) 27 (39%) <0.0001 Stage (IA />IA) 78/65 58/15 20/50 <0.0001
Beta-2 Microglobulin and Soluble Interleukin-2 Receptor Serum Levels Correlates with Human Immunodeficiency Virus Associated Non-Hodgkins Lymphoma-Diffuse Large B-Cell Lymphoma (HIV-NHL-DLBCL).
Authors: McPherson Eugene , Ng J. , Hazel E. , Tassy P. 
Published: October 2019

Abstract
Beta-2-microglobulin (B2M) is a cell surface protein on many somatic cells including T and B lymphocytes and macrophages as a subunit of class I major histocompatibilty complex (MHC). Soluble interleukin-2 receptor (sIL-2R) is a T cell derived cytokine that induce proliferation of activated T cells, participates in differentiation of B cells and modulates macrophage function and phenotype. Both serum B2M and sIL-2R levels reflect immune-system activation and are elevated in patients with HIV-NHL-DLBCL. Combined detection of B2M and sIL-2R can be used to predict treatment response or failure in patients with aggressive HIV-NHL-DLBCL. When highly active antiretroviral therapy (HAART) chemotherapy is administered to refractoriness of elevated B2M and sIL-2R serum levels may be seen in HIV-NHL-DLBCL poor responders or failures. As sIL-2R levels decrease, B2M levels remain elevated in early stage HIV-NHL-DLBCL. Treatment of these patients with CHOP-rituximab and HAART both markers decrease. However, in late stage disease (stage III-IV, HIV-NHL-DLBCL based on DLBCL), B2M and sIL-2R remain significantly elevated and prognosis poor even after immune restoration of CD4+ T cell count and HIVRNA viral load approach normal.
CASE: We present one of several cases - a 38 year old HIV positive male with DLBCL of his sinus turbinates, had turbinectomy surgery with sIL-2R level of 617 U/mL (5,460.226 pg/ml) postop which gradually increased to 27,673.95 pg/ml in less than six months. The B2M level while on HAART decreased from 18.8 mg/L to 4.4 mg/L. His LDH, CRP, and GGT levels were elevated but the CD4+ T cell count was 26% and his HIVRNA viral load became undetectable. After CHOP-rituximab therapy sIL-2R decreased to 10,806 pg/ml from 27,674 pg/ml but slowly rose to 39,975.25 pg/ml and B2M increased to 19 mg/L. The CD19+ B cell count was zero and a CD4/CD8 ratio improve to 0.546.
CONCLUSION: B2M and sIL-2R serum level measurements provides data that when used together may offer a better management approach to these HIV-NHL-DLBCL patients. B2M and sIL-2R elevated refractory levels correlates with tumour burden and predicts poor prognosis in aggressive HIV-NHL-DLBCL.
The Roles of Pten in Hematopoietic Stem Cell Regulation and Leukemogenesis.
Authors: Zhang Jiwang , He Xi , Jayasinghe Sach , Ross Jason , Haug Jeff , Li Linheng 
Published: October 2019

Abstract
Pten was the first phosphatase identified as a tumor suppressor and one of the most frequently mutated genes involved in human tumor/cancer. Pten, involved in regulation of both PI3K/Akt and MEK/Erk activity, is downstream of growth factor, cytokine, integrin and cadherin signaling pathways and therefore plays important roles in cell growth, survival, differentiation, metabolism and migration. Although Pten mutation is not common in leukemic cells, phosphorylated Pten (p-Pten), which represents the inactive form of Pten, has been observed in a majority of acute myeloid leukemias that are associated with poor clinical outcomes. To explore the role of Pten in hematopoietic stem cell (HSC) regulation and leukemogenesis, we generated an interferon-inducible Pten knockout mouse by crossing Mx1Cre mice with Ptenloxp mice. All of the mutant mice developed myeloproliferative disorder characterized by increased peripheral white blood cell counts, hyperproliferative macrophages and granulocytes in bone marrow and spleen, and multiple tissue infiltration by myeloid cells. The HSC number was decreased in the bone marrow but mobilized and expanded in the spleen. Extra-medullar hematopoiesis was shown by dramatically increased myeloid and erythroid progenitors in the spleen. B lymphocyte differentiation was blocked at the common lymphoid progenitor stage, while the T cell number was increased in all hematopoietic tissues. Compared to wild type, Pten mutant HSCs and progenitor cells were highly proliferative, forming larger colonies in an in vitro culture study. However, Pten mutant HSCs showed reduced competency in repopulation assay after in vivo bone marrow transplantation. Our study demonstrates that Pten plays important roles in restricting HSC activation, proliferation and mobilization. Pten also plays a role in HSC lineage decision by favoring myeloid differentiation at the expense of B lymphocyte lineage.
Evaluation of the Oncohaematologic Pathology of Two Oncologics Centers of Buenos Aires, Argentina in the Period between 1995–2005; a Total of 1884 Cases.
Authors: Dragosky Marta O. , Annetta Isabel , Alvarellos4 Viviana , Devotto5 Laura , Luchetta6 Patricia , Marquez7 Marisa , Alcaraz Susana 
Published: October 2019

Abstract
Introduction: In the absence of national and regional records of oncohaematologic pathology the information regarding the composition and characterstics of this patients population was evaluated in this two oncologic centers of Buenos Aires.
Results: Of the whole of 1884 patients, the distribution of the pathologies was: Non Hodgkin Lymphoma (NHL): 1040 cases (55,2 %), Hodgkin Lymphoma (HL) 358 (19 %), Leukemia 258 (13,7 %), (chronic lymphatic 105, chronic myeloid 93, acute 52, trycholeukemia 8) Myeloma 131 (6,95 %), thrombocythemia 24 (1,27 %), Myelodysplasia 22 (1,16 %), Polycythemia Vera: 17 (0,90 %), Plasmacytoma 14 (0,74 %), Myelofibrosis 8 (0,42 %), Histiocytosis. 5 (0,26 %), Macroglobulinemia of Waldenstrom 4 (0,2 %), Granulocytic Sarcoma 2 (0,1 %), Dendritics Cells Sarcoma 1 (0,05 %).
The sex distribution was: 982 (52.14 %) males, 902 (47,86 %) females. The range of ages was: 15 to 94 years, with an average of 52,3 years.
Of the whole of 1040 NHL 274 (26,34 %) presented extranodal locations, distributed as follows: skin 63, orbital 37, tonsil 35, oral cavity 17, stomach 16, central nervous system 16 (12 brain), breast 13, soft tissues 12, parothyd 11, cavum 11, bowel 9, testicle 7, paranasal sinuses and bones 6, rectum and lung 3, larynx, uterus and scalp 2, liver, ovary and thyroid gland 1. A whole of 29 patients presented pathology associated with the HIV: 22 NHL and 7 HL.
In the population with HL the average age was 36,7 years, with 38 % younger than 25 years, we do not observe the bimodal curve usually descript.
The predominant histologies: nodular sclerosis 40,7 % and mixed cellularity36,53 % (low frequency of subtypes lymphocitary predominance and lymphocitary depletion). Stage II 44,87 % and stage III 23,71 %. The most frequent locations: cervical 53,2 % and mediastinic 47,11 % (and low frequency of abdominal and inguinal locations).
Conclusions: The frequency of extranodal locations of NHL is similar as the international records. The distribution of the unusual locations is probably biased by the pathologies arrived from specific centers (for example: a center of ophthalmology). The population of HL is characterized by the young age and the supradiaphragmatic locations
Hepatitis C Viral Infection Is Not Associated with Waldenstrom’s Macroglobulinemia.
Authors: Leleu Xavier , O’Connor Kelly , Ho Allen , Santos Daniel D. , Manning Robert , Xu Lian , Hatjiharissi Evdoxia , Tournilhac Olivier , Chemaly Mariana , Branagan Andrew R. , Hunter Zachary R. , Patterson Christopher J. , Anderson Kenneth C. , Treon Steven 
Published: October 2019

Abstract
Infection with hepatitis C virus (HCV) has previously been associated with the pathogenesis of low grade B-cell lymphoproliferative diseases including Waldenstrom’s macroglobulinemia (WM). Most of these studies have utilized HCV antibody detection assays, which may not accurately assess HCV presence in patients with impaired humoral responses. In patients with WM, pronounced IgA and IgG hypogammaglobulinemia is present, which remains persistant despite therapy (Blood 104:306b). As such, we investigated the incidence of HCV in 88 randomly selected, and previously untreated patients with the consensus panel diagnosis of WM utilizing both HCV antibody detection and qualitative PCR assays (Quest Diagnostics, Cambridge, MA, USA). The median age for these patients was 61 (38 – 83 years) and the male: female sex ratio was 1.44. No patient had a known history of liver disease, hepatitis B, HCV or HIV infection. Liver function tests obtained at time of serum collection showed normal range SGOT and and SGPT levels for 86/88 patients. In contrast to previous reports, we failed to demonstrate HCV infection by both HCV antibody detection and the PCR assays. These studies therefore show no association of WM with HCV infection.
Follicular Lymphoma of the GI Tract - Distinct Entity or Extranodal Manifestation of Nodal Disease?.
Authors: Mehta Sharmila , Kulharya Anita , Kallab Andre , Mazzella Fermina M. 
Published: October 2019

Abstract
Introduction: Primary GI follicular lymphoma is a rare, poorly studied entity. Current discussions in the literature debate whether or not it is a distinct extranodal entity, similar to cutaneous follicle center lymphoma. Like cutaneous follicle center lymphoma, it appears to have a good prognosis and low incidence of t(14;18).
Case History: This is a 74 year old white male who presented for routine screening colonoscopy in May 2003. On evaluation the patient was found to have nodularity of the colon. Biopsies demonstrated several submucosal and mucosal follicles with no distinct mantle zones, composed of a uniform population of centrocytes. No lymphoepithelial lesions or plasma cells were identified. These cells proved to be monoclonal B cells, with a phenotype of CD20+, CD10−/+, bcl-2+/−, bcl-6+, CD5−, CD43−, lambda+ and t(14;18) negative. CD21 stain highlighted the underlying follicular dendritic architecture. No adenopathy was noted at this time. A diagnosis of B cell lymphoma, consistent with extranodal follicular lymphoma, grade I was rendered. As the patient was otherwise asymptomatic, no treatment was given. Four months later, the patient developed retroperitoneal adenopathy and a large pelvic mass. A lymph node biopsy demonstrated numerous large follicles composed of a uniform population of centrocytes, with attenuated to absent mantle zones. The cells were CD20+, CD10+, bcl-2+, lambda+ and t(14;18) positive. There was a comcomitant transitional cell carcinoma of the urinary bladder, which required radical cystectomy. The patient was then initiated on chemotherapy with CVP, for three cycles. He was followed without treatment for 2 years when he developed a tonsillar mass. A biopsy demonstrated a CD10+ diffuse large B-cell lymphoma. Cytogenetic analysis revealed a complex karyotype with a t(14;18). The patient underwent a staging bone marrow, which was positive for follicular lymphoma. The cells were CD20+, CD10−/+, bcl-6+, lambda+, and t(14;18) negative. He was then started on R-CHOP for his B symptoms and extensive adenopathy, and is currently 2 months into therapy.
Discussion: This is an unusual case of almost concomitant presentation of t(14;18) negative extranodal follicular lymphoma of the GI tract and classical t(14;18) positive nodal follicular lymphoma. Possible explanations include that these are two manifestations of the same disease, two separate diseases coincidentally occurring around the same time, or a follicular lymphoma clone which has evolved into nodal and extranodal entities.
Collagen Type IV as Independent Prognostic Factor for Non-Hodgkin’s Lymphoma.
Authors: Hadzi-Pecova Liljana , Petrusevska Gordana , Panovska Irina , Stankovic Svetlana , Stojanovik Aleksandar 
Published: October 2019

Abstract
Many attempts have been made recently to improve the prognostic systems for non-Hodgkin’s lymphoma (NHL). There are no available data in the literature regarding the influence of intactness of the basement membrane of the blood vessels on the prognosis of NHL, when compared with the clinical characteristics of the disease. On the other hand, it is known that the integrity of the basement membrane is marker for the aggressiveness of the malignant disorders.
In order to analyze the association of the expression patterns of collagen type IV on the prognosis of the patients with NHL we have conducted a retrospective study.
We have evaluated 136 patients diagnosed and treated as NHL in the last ten years at the Clinic of Hematology, Medical Faculty -Skopje. All 136 patients with representative tumor tissues were diagnosed with NHL according to the REAL classification (71 patients were diagnosed as diffuse large cell lymphoma, 28 as follicular lymphoma, 24 as small lymphocytic lymphoma and 14 as marginal zone lymphoma). Median age of the patients was 49 years, with male: female ratio 1.5:1.0. The application of international prognostic index (IPI) identified four risk groups with predicted five-years survival rates of 87%, 82%, 18% and 0%, with statistical significance (p< 0.001).
Using immunoperoxidase staining we have analyzed the collagen type IV expressing patterns in our group of patients, and defined three tumor invasive grades: high tumor invasive grade as total lack of immunoreactivity for collagen type IV (68 patients), medium-presented as discontinuous expression (56 patients) and low-as a continuous linear expression (14 patients). The 5 year overall survival rate was significantly lower (p<0.01) in patients with high invasive grade than in patients with medium or low invasive grade (54% vs. 78% and 100%). Further, in multivariate analysis collagen type IV expression appeared as an independent survival-predicting factor together with the parameters of the IPI.
Our results indicated that the expression of a collagen type IV immunoreactivity could predict the outcome of patients with NHL. However, further studies are needed to define the role of collagen type IV in the future prognostic systems for NHL.
Expression of the BAFF-R (BR3) Is Positive in a Minority of Diffuse Large B Cell Lymphomas and Does Not Correlate with the Germinal Center or Non Germinal Center Origin of Lymphomatous Cells.
Authors: Proust Alexis , Adrar Idir , Rince Patricia , Mercier Virginie , Lazure Thierry , Raphael Martine , Garçon Loïc 
Published: October 2019

Abstract
BAFF, a member of the TNF family, has been shown to be implicated in B cell maturation and survival through an interaction with its three receptors, BCMA, TACI and BAFF-R. Recent data revealed that it also plays a role in B cell malignancies. For exemple, an autocrine activation of the BAFF receptors could participate to oncogenesis during multiple myeloma or chronic lymphocytic leukemia. BAFF-R, the sole BAFF specific receptor, is expressed in a subset of Diffuse Large B Cell Lymphomas (DLBCL). Since BAFF-R activation in B cells leads to cell proliferation and survival, expression of this receptor in DLBCL could correlate with more aggressive lymphomas. DLBCL can be divided into prognostically important subgroups with germinal center B cell-like (GCB), activated B cell-like (ABC) and type 3 gene expression profiles using a cDNA microarray. The germinal center origin of malignant B cells in DLBCL can also be characterized by immunohistochemistry (IHC) according to CD10, Bcl6 CD138 and MUM1 expression. In this work, we investigated whether expression of BAFF-R in DLBCL correlate or not with the GCB vs non GCB phenotype of lymphomatous cells. Lymph nodes biopsies from 23 DLBCL (among whom 3 post-transplantation lymphomas and 1 AIDS-related DLBCL) were analyzed. In a first step, we characterized by IHC the precise phenotype of each DLBCL: CD10+/Bcl6+ DLBCL were classified into the germinal center (GCB) group (n=9), whereas CD10-/BCL6-/MUM1+ lymphomas defined the non germinal center (NGCB) one (n=14). Each DLBCL was then analyzed for BAFF-R expression by IHC. In agreement with a recent report, most of the DLBCL were found to be negative for BAFF-R expression (16 out of 23). Interestingly, 4 of the 9 GCB DLBCL were found to be BAFF-R positive (44%), whereas only 3 out of 14 (21%) in the NGCB group. Although the difference did not reach statistical significance, we concluded from this observation that a consequent percentage of GCB DLBCL express the specific receptor BAFF-R. Thus, we actually analyze the clinical and biological characteristics of BAFF-R positive DLBCL and investigate whether expression of BAFF-R could represent a bad prognosis factor in term of clinical outcome and response to treatment.
Analysis of Nuclear Factor Kappa B in Mantle Cell Lymphoma.
Authors: Schrader Carsten , Klapper Wolfram , Janssen Dirk , Riis Paul , Meusers Peter , Brittinger Guenter , Siebmann Jens U. , Kneba Michael , Parwaresch Reza 
Published: October 2019

Abstract
Gene expression profiles revealed that proliferation associated genes are important prognostic factors in the clinical outcome in mantle cell lymphoma (MCL). Beside this well accepted markers also analysis of apoptotic proteins are now under investigation. We investigated immunohistochemically the expression of the apoptotic marker NF-kB in relation to the clinical course in 89 patients enrolled in two multicenter prospective trials. Biopsies were recut and stained with mandatory antibodies (CD20, CD5, CD3, CD23, cyclin D1) and NF-kB. The NF-kB expression was analyzed in three groups: negative, cytoplasmatic positive and nuclear positive (more than 1/HPF). The expression was compared with the overall survival data analyzed according to Kaplan and Meier. In 13 cases a negative NF-kB staining was detected. Fifty-seven cases were positive only in the cytoplasma and in 17 cases more than 1 cell per high power field showed nuclear activity. Patients with mantle cell lymphoma that had negative and cytoplasmatic positive expression had a median overall survival time of 35.7 months compared to 22.4 months for patients with a nuclear NF-kB expression. The Kaplan-Meier analysis showed a significant difference in the overall survival time (p=0.0121). The immunohistochemical detection of NF-kB in mantle cell lymphoma is possible and a tool to identify patients with a poor prognosis.
Value of Early Evaluation with FDG-PET for Follicular Lymphoma.
Authors: Gressin Remy R. , Vuilliez Jean Philippe J.P. , Molina Lysiane L. , Pegourie Brigitte B. , Courby Stephane S. , Garban Frederic F. , Bulabois Claude Eric C.E. , Orsini Frederique F. , Nicolas Emmanuelle E. , Makowski Caroline C. , Sotto Jean Jacques J.J. , Cahn Jean Yves J.Y. 
Published: October 2019

Abstract
Objective: evaluate the predictive value of the FDG-PET on the evolution of Follicular lymphomas.
Methods: FDG-PET was performed in 17 consecutive patients treated for follicular lymphoma during the course of their treatment (ttt): 8 were evaluate early (Ea) after the second course of chemotherapy (CT) and 9 later (La) after the third course (5 pts) or the fourth (4 pts). 9 were treated at diagnosis and 8 at relapse. 15 histologic grade 1–2 received 6 courses of Rituximab-VAD-Chlorambucil (n=13) or R-miniCHVP (n=2) and two grade 3 a R-CHOP like regimen. 12 were intensified with haematopoietic stem cell transplantation (10 auto, 2 allo). 6 were Flipi 0–1, 3 Flipi 2 and 8 Flipi 3–4.
Results:
Among the 8 Ea, only 3 negativate their FDG-PET, 4 had a minimal uptake and one remained positive (he relapsed 2 months after the completion of their ttt). 4/4 pts with minimal uptake became negative after the completion of the ttt. 7/7 negative pts remained relapse free (Median Fup 17 months).
Among the 9 La, 8 were negative (5/5 after the third course) and one had a residual uptake after the fifth course (he died disease free from non haematolgical cause). One/8 negative patient have relapsed 5 months after ttt, 7 are alive and disease free. The 2 histologic grades 3 were Ea. Negative. Flipi 0–1(n=6) or Flipi 2 (n=3) were all negative. The Ea. Positive and the La. Residual were Flipi 3.
Conclusion
The best time of evaluation for the follicular lymphoma might be later than for High grade and might be proposed after three courses of chemotherapy when associated with rituximab. The Flipi seems to be correlated to the FDG-Pet response. These preliminary results are encouraging to define the predictive value of FDG-PET for follicular lymphoma survival, especially for the flipi 3.