bluuebunny/crossref_metadata_2025_split huggingface dataset:
Suppression of PRPF4 regulates pluripotency, proliferation, and differentiation in mouse embryonic stem cells
Authors: Park Song , Han Se‐Hyeon , Kim Hyeon‐Gyeom , Jeong Jain , Choi Minjee , Kim Hee‐Yeon , Kim Min‐Gi , Park Jin‐Kyu , Han Jee Eun , Cho Gil‐Jae , Kim Myoung Ok , Ryoo Zae Young , Choi Seong‐Kyoon 
Published: September 2019

Mouse embryonic stem cells (mESCs) are characterized by their self‐renewal and pluripotency and are capable of differentiating into all three germ layers. For this reason, mESCs are considered a very important model for stem cell research and clinical applications in regenerative medicine. The pre‐mRNA processing factor 4 (PRPF4) gene is known to have a major effect on pre‐mRNA splicing and is also known to affect tissue differentiation during development. In this study, we investigated the effects of PRPF4 knockdown on mESCs. First, we allowed mESCs to differentiate naturally and observed a significant decrease in PRPF4 expression during the differentiation process. We then artificially induced the knockdown of PRPF4 in mESCs and observed the changes in the phenotype. When PRPF4 was knocked down, various genes involved in mESC pluripotency showed significantly decreased expression. In addition, mESC proliferation increased abnormally, accompanied by a significant increase in mESC colony size. The formation of mESC embryoid bodies and teratomas was delayed following PRPF4 knockdown. Based on these results, the reduced expression of PRPF4 affects mESC phenotypes and is a key factor in mESC.Significance of the studyOur results indicate that PRPF4 affects the properties of mESCs. Suppression of PRPF4 resulted in a decrease in pluripotency of mESC and promoted proliferation. In addition, suppression of PRPF4 also resulted in decreased apoptosis. Moreover, the inhibition of PRPF4 reduced the ability to differentiate and formation of teratoma in mESC. Our results demonstrated that PRPF4 is a key factor of controlling mESC abilities.
Dysregulated interleukin 11 in primary Sjögren's syndrome contributes to apoptosis of glandular epithelial cells
Authors: Ye Lei , Shi Huan , Wu Shufeng , Yu Chuangqi , Wang Baoli , Zheng Lingyan 
Published: September 2019

AbstractThe purpose of this study was to explore the potential function of interleukin‐11 (IL‐11) in the pathogenesis of primary Sjögren's syndrome (pSS) patients. Real‐time polymerase chain reaction was performed to examine IL‐11 expression in the labial glands of 30 pSS patients and 30 healthy controls. Immunohistochemistry was conducted to assess the distribution of IL‐ll‐positive cells in labial glands. The human salivary gland (HSG) cell line was used to study the effects of IL‐11 on gland epithelial cells in vitro. Cell viability and cell proliferation were examined by CCK‐8 kit and EdU assay, respectively. The population of apoptotic cells was detected in flow cytometry followed by Annexin V/PI and Hoechst staining. We found that the expression levels of IL‐11 were remarkably decreased in pSS labial glands and were positively correlated with C‐reactive protein levels and negatively correlated with rheumatoid factor levels. Fewer numbers of glandular epithelial cells were observed to be positively stained with IL‐11 antibody in labial glands from pSS patients than those in healthy control patients. After IL‐11 treatment, the viability and proliferation of HSG cells were significantly higher than those in the control group. The total apoptotic and necrotic rates of HSG cells in the group after IL‐11 treatment were significantly lower. In conclusion, the results indicated that IL‐11 promoted viability and proliferation and inhibited apoptotic and necrotic rates of glandular epithelial cells. In pSS, downregulated IL‐11 might contribute to the apoptosis of salivary gland epithelial cells. However, it might be a potential target to alleviate the pathological atrophy of glandular epithelial cells in pSS patients.
Heat shock protein 27 as a neuroprotective biomarker and a suitable target for stem cell therapy and pharmacotherapy in ischemic stroke
Authors: Behdarvandy Marjan , Karimian Mohammad , Atlasi Mohammad Ali , Azami Tameh Abolfazl 
Published: September 2019

AbstractIschemic stroke is a major common cause of death and long‐term disability worldwide. Several pathophysiological events including excitotoxicity, oxidative/nitrative stress, inflammation, and apoptosis are involved in ischemic injuries. Recently, the molecular mechanisms involved in cerebral ischemia through a focus on a member of small heat shock proteins family, Hsp27, has been developed. Notably, following exposure to ischemia, Hsp27 expression in the brain could be increased rather than the normal condition and it may play an important role in neuroprotection after ischemic stroke. The neuroprotection effects of Hsp27 may arise from its anti‐oxidant, anti‐inflammatory, anti‐apoptotic, and chaperonic properties. Moreover, some therapeutic strategies such as stem cell therapy and pharmacotherapy have been developed with Hsp27 targeting. In this review, we describe the function and structure of Hsp27 and its possible role in neuroprotection after ischemic stroke. Finally, we present current studies in stroke therapy, which focused on Hsp27 targeting.
A case of carcinoma ex pleomorphic adenoma arising from multinodular pleomorphic adenoma of the buccal region
Authors: Yamamoto Koji , Sato Yuichiro , Kondo Yuudai , Tanaka Hiroyuki , Akiyama Yutaka , Yamashita Yoshihiro , Kataoka Hiroaki 
Published: September 2019

AbstractIn multinodular lesions or tumors with mixed benign and malignant components, malignant elements may be undetectable using fine‐needle aspiration biopsy/cytology in preoperative pathological diagnosis of some cases, because of sampling error.
Orthogonally Incorporating Dual‐Fluorescence Control into Gated Photochromism for Multifunctional Molecular Switching
Authors: Weng Taoyu , Zhang Kai , Wu Bin , Chen Xuanying , Zou Qi , Zeng Tao , Zhu Liangliang 
Published: September 2019

AbstractGated photochromism is of interest for the operation and control of modern high‐tech optofunctional materials. For further advancing this topic towards the achievement of multifunctional molecular switching, however, it remains a great challenge to incorporate multiple fluorescence regulation into gated photochromism in one unimolecular system. Herein, it is reported that a dithienylethene derivative DTEN with a Schiff base connection can be facilely synthesized by one‐step coupling, and it enabled distinct color and spectral changes upon different stimuli, including ultraviolet, visible light, Ni2+, and Al3+. Relying on hydrazine and hydroxy units in this molecule, compound DTEN exhibited novel Ni2+‐locked photochromic characteristics originating from complexation of the compound with Ni2+ in a 2:1 stoichiometry. On the other hand, a 1:1 complexation between compound DTEN and Al3+ could allow both of the initial and photostationary states of DTEN to display fluorescent enhancement and a redshift, realizing a dual‐fluorescence “turn‐on” sensing of Al3+ by light. On this basis, it is argued that the switching of the coordination mode between DTEN and Ni2+ or Al3+ brings up the possibility of tunable photoswitching by multiple stimuli, which offers a novel way for future development of multifunctional switching materials with different input and output signals, as exemplified by the construction of a delicate molecular circuit.
Stafia‐1: a STAT5a‐Selective Inhibitor Developed via Docking‐Based Screening of in Silico O‐Phosphorylated Fragments
Authors: Natarajan Kalaiselvi , Müller‐Klieser Daniel , Rubner Stefan , Berg Thorsten 
Published: September 2019

AbstractWe present a new approach for the identification of inhibitors of phosphorylation‐dependent protein–protein interaction domains, in which phenolic fragments are adapted by in silico O‐phosphorylation before docking‐based screening. From a database of 10 369 180 compounds, we identified 85 021 natural product‐derived phenolic fragments, which were virtually O‐phosphorylated and screened for in silico binding to the STAT3 SH2 domain. Nine screening hits were then synthesized, eight of which showed a degree of in vitro inhibition of STAT3. After analysis of its selectivity profile, the most potent inhibitor was then developed to Stafia‐1, the first small molecule shown to preferentially inhibit the STAT family member STAT5a over the close homologue STAT5b. A phosphonate prodrug based on Stafia‐1 inhibited STAT5a with selectivity over STAT5b in human leukemia cells, providing the first demonstration of selective in vitro and intracellular inhibition of STAT5a by a small‐molecule inhibitor.
Impact of assertive and defensive impression management tactics on recruiter evaluation: The case of post‐failure entrepreneurs in employment interviews
Authors: Cusin Julien , Passebois‐Ducros Juliette 
Published: September 2019

AbstractThis study investigates how human‐resource professionals examine, during a job interview, the application of post‐bankruptcy entrepreneurs seeking to rebound as salaried workers. We assume that the way candidates talk about their failure (impression management tactics—IM) impacts the assessment of the recruiter. Thanks to an experimental methodology we compare the impact of three distinct IM tactics on the assessment of the hiring manager (a defensive IM tactic based on excuses, a defensive IM tactic based on justification, and an assertive IM tactic). We demonstrate that recruiters are more inclined to recruit the post‐bankruptcy entrepreneur if the candidate has been able to turn the ordeal into an element of self‐valorization.
Comparative toxicity outcomes of proton‐beam therapy versus intensity‐modulated radiotherapy for prostate cancer in the postoperative setting
Authors: Santos Patricia Mae G. , Barsky Andrew R. , Hwang Wei‐Ting , Deville Curtiland , Wang Xingmei , Both Stefan , Bekelman Justin E. , Christodouleas John P. , Vapiwala Neha 
Published: September 2019

BackgroundDespite increasing utilization of proton‐beam therapy (PBT) in the postprostatectomy setting, no data exist regarding toxicity outcomes relative to intensity‐modulated radiotherapy (IMRT). The authors compared acute and late genitourinary (GU) and gastrointestinal (GI) toxicity outcomes in patients with prostate cancer (PC) who received treatment with postprostatectomy IMRT versus PBT.MethodsWith institutional review board approval, patients with PC who received adjuvant or salvage IMRT or PBT (70.2 gray with an endorectal balloon) after prostatectomy from 2009 through 2017 were reviewed. Factors including combined IMRT and PBT and/or concurrent malignancies prompted exclusion. A case‐matched cohort analysis was performed using nearest‐neighbor 3‐to‐1 matching by age and GU/GI disorder history. Logistic and Cox regressions were used to identify univariate and multivariate associations between toxicities and cohort/dosimetric characteristics. Toxicity‐free survival (TFS) was assessed using the Kaplan‐Meier method.ResultsThree hundred seven men (mean ± SD age, 59.7 ± 6.3 years; IMRT, n = 237; PBT, n = 70) were identified, generating 70 matched pairs. The median follow‐up was 48.6 and 46.1 months for the IMRT and PBT groups, respectively. Although PBT was superior at reducing low‐range (volumes receiving 10% to 40% of the dose, respectively) bladder and rectal doses (all P ≤ .01), treatment modality was not associated with differences in clinician‐reported acute or late GU/GI toxicities (all P ≥ .05). Five‐year grade ≥2 GU and grade ≥1 GI TFS was 61.1% and 73.7% for IMRT, respectively, and 70.7% and 75.3% for PBT, respectively; and 5‐year grade ≥3 GU and GI TFS was >95% for both groups (all P ≥ .05).ConclusionsPostprostatectomy PBT minimized low‐range bladder and rectal doses relative to IMRT; however, treatment modality was not associated with clinician‐reported GU/GI toxicities. Future prospective investigation and ongoing follow‐up will determine whether dosimetric differences between IMRT and PBT confer clinically meaningful differences in long‐term outcomes.
PFG‐NMR as a Tool for Determining Self‐Diffusivities of Various Probe Molecules through H‐ZSM‐5 Zeolites
Authors: Bingre Rogéria , Losch Pit , Megías‐Sayago Cristina , Vincent Bruno , Pale Patrick , Nguyen Patrick , Louis Benoît 
Published: September 2019

AbstractThe understanding of major zeolite applications is partially based on diffusion of molecules inside or outside microporous networks. However, it is still a challenge to measure such phenomena. The diffusion ordered nuclear magnetic resonance spectroscopy (DOSY) technique has been reported to measure a probe molecule‘s diffusion inside porous solids. Pulsed‐field gradient (PFG)‐NMR has been used herein to measure the self‐diffusivity of different probe molecules, such as neopentane, benzene, toluene and 1‐dodecene with increasing dynamic diameter, respectively, on a series of H‐ZSM‐5 zeolites. The latter materials exhibit different crystal sizes, Si/Al ratios and the presence (or absence) of crystalline defects. In addition, shaped zeolite bodies representing industrial catalysts were compared with the afore‐mentioned samples.
Secondary Stroke Prevention: A Population‐Based Cohort Study on Anticoagulation and Antiplatelet Treatments, and the Risk of Death or Recurrence
Authors: Faure Mareva , Castilloux Anne‐Marie , Lillo‐Le‐Louet Agnès , Bégaud Bernard , Moride Yola 
Published: September 2019

Using claims databases of a public healthcare program (Quebec) for the years 2010–2013, we conducted a cohort study of patients with acute ischemic stroke (AIS) to describe secondary prevention treatments and determine how they stood against practice guidelines. We compared the risk of death or AIS recurrence over 1 year in patients treated with anticoagulants, antiplatelets, and/or other cardiovascular drugs. In the month after discharge, 44.3% of the patients did not receive the recommended treatment and > 20% did not have any treatment. Untreated patients were younger, had less comorbidities, and a more severe AIS. Anticoagulants and antiplatelets were associated with a reduced risk of death or recurrence (hazard ratio (HR) 0.27; 95% confidence interval (CI) 0.20–0.36 and HR 0.25; 95% CI 0.16–0.38, respectively) compared with the untreated group. Effect size was similar for the other treatments. Findings confirm treatment benefits shown in clinical trials and emphasize the importance of AIS secondary prevention.
Design and analysis of CNTFET based 10T SRAM for high performance at nanoscale
Authors: Kumar Mukesh , Ubhi Jagpal Singh 
Published: September 2019

SummaryThe proposed 10T SRAM cell design is implemented for different CNTFET parameters like pitch, number of tubes, chirality, dielectric materials, and flatband voltage to analyze its effect on various performance parameters. The channel gate width, average read, and write power increase, but leakage power, read delay, and write delay decrease with the increase in pitch of CNTFET, whereas all these parameters are directly proportional to the number of tubes. Chirality alteration shows inverse effect on threshold voltage, read delay, and write delay although other parameters are directly related to it. The performance parameters are evaluated for various dielectric materials, and HfO2 gives the best results for low power and high‐speed applications. Analysis of flatband voltage on proposed 10T SRAM is performed by keeping flatband voltage constant for n‐CNTFET and varied for p‐CNTFET. Extensive analysis has been done to scrutinize the sharing of powers and delay of 10T SRAM because of variations in supply voltage and temperature. The supply voltage sweeps for a range between 0.6 and 1.2 V, and range of temperature variation is considered from −27 to 127°C. The stability of the proposed SRAM cell is calculated using N‐curve method to find voltage and current information. The CNTFET based 10T SRAM cell depicts that it persists supply voltage and temperature variation significantly superior than CMOS.
Sexually dimorphic behavioral effects of maternal separation in anorexic rats
Authors: Farinetti Alice , Aspesi Dario , Marraudino Marilena , Marzola Enrica , Amianto Federico , Abbate-Daga Giovanni , Gotti Stefano 
Published: September 2019

AbstractExposure to negative events during the neonatal period is one of the leading factors contributing to the development of psychiatric disorders, including anorexia nervosa. In this study, we investigated the effects of maternal separation (MS) on the development of anorexia in rodents using the mild‐stress form of the activity‐based anorexia (ABA) model (2 hr of free access to a running wheel and a 1‐hr feeding test) in both male and female rats. We assessed anxiety‐like and locomotor behavior and hyperactivity with the open field and elevated plus maze tests. Our results showed that ABA rats of both sexes displayed hyperactive behavior associated with reduced anxiety‐like behavior when compared to controls. However, a sexually dimorphic effect of MS emerged in anorexic rats: while the females exposed to MS + ABA were hyperactive with diminished anxiety‐related behaviors compared to females of the ABA group, MS in males attenuated or did not alter the effects of the ABA protocol. In conclusion, our data reveal that the synergistic effects of MS and ABA on physical activity and anxiety‐like behavior act in opposite directions in the two sexes.
Highly Selective and Sensitive Electrochemical Determination of Ni(II) in Real Samples Based on Ion‐imprinted Polymer Technology
Authors: Razmi Habib , Dehghanzade Mahsa 
Published: September 2019

AbstractNickel is one of the heavy metals which has been considered as a serious pollutant affecting the human health. Therefore, it is necessary to control its concentration in foods and drinks. Recently, ion‐imprinted polymer based electrochemical sensors have been attracted lots of attention due to their valuable selectivity and sensitivity toward target analytes. In this study, Ni(II)‐1,10‐phenanthroline complex was prepared in ethanol and added stepwise to the aqueous solution of dopamine, followed by pH adjustment for self‐polymerization of dopamine in alkaline conditions. During the polymerization process, Ni(II)‐imprinted polydopamine (Ni‐PDA) was formed due to effective interactions between polydopamine and 1,10‐phenanthroline ligands carrying Ni(II) ions. A thin film of Ni‐PDA was anchored on a glassy carbon electrode (GCE) through a simple casting procedure. After drying, the sensor was employed for the determination of Ni(II) ions by means of differential pulse voltammetry. Under optimum conditions, a linear calibration curve was obtained in the range 1–25 μM with the detection limit of 0.39 μM (S/N=3). Due to excellent selectivity to Ni(II) ions, the sensor was not suffered from the excess amount of interfering ions and used for Ni(II) determination in different water and tea samples.
Enhanced Electrocatalytic Oxidation of Paracetamol at DNA Modified Gold Electrode
Authors: Althagafi Ismail I. , Kassem Mohammed A. , Awad Mohamed I. 
Published: September 2019

AbstractCysteine monolayer has been assembled onto bare gold electrode (SAM/Au), and subsequently deoxyribonucleic acid (DNA) has been successfully immobilized at the SAM/Au electrode. The thus modified electrode is assigned DNA/SAM/Au. Modification steps of the electrode were followed electrochemically using K4[Fe(CN6)] electrochemical marker. Also, the build‐up of the modified electrode composition is followed using EDX and the crystallographic orientation is inspected using XRD. The electrochemical behavior of paracetamol (PC) at DNA/SAM/Au electrode is investigated. Interestingly, the sluggish irreversible behavior of PC at the bare gold electrode is converted to a quasi‐reversible one at DNA/SAM/Au electrode pointing to some interaction between the immobilized DNA and PC. The enhanced electrochemical behavior of PC at modified DNA/SAM/Au electrode is successfully used for a sensitive electrochemical determination of PC. Square wave voltammetry (SWV) was used for this purpose. The concentration of PC was in linear relation with the peak current at the optimum conditions within the range 10.0–110.0 μg mL−1 with correlation coefficient (R2) of 0.998. Also, the standard deviation (SD) and relative standard deviation (RSD) were calculated and found to be 0.817 and 1.52, respectively, indicating the significance of the present method.
Pulsed Deposited Manganese and Vanadium Oxide Film Modified with Carbon Nanotube and Gold Nanoparticle: Chitosan and Ionic Liquid‐based Biosensor
Authors: Akoğulları Seçil , Çιnar Seda , Özdokur Kemal Volkan , Aydemir Tülin , Ertaş Fatma Nil , Koçak Süleyman 
Published: September 2019

AbstractPresent study describes the synthesis of mixed oxide films of manganese and vanadium by electrochemical pulsed deposition technique on a glassy carbon electrode (GCE) modified with multiwall carbon nanotubes (MWCNT). The film was further decorated with gold nanoparticles to enhance the reduction signal of dissolved oxygen in pH 5.17 acetate buffer solution. All of the electrochemical synthesized modified electrodes have been characterized with Scanning electron microscopy(SEM), High‐resolution transmission electron microscopy (HRTEM), X‐Ray photoelectron spectroscopy (XPS), X‐Ray diffraction (XRD) techniques. The electrode obtained (AuNPs/MnOx−VOx/CNT/GCE) was utilized as a platform for glucose biosensor where the glucose oxidase enzyme was immobilized on the composite film with the aid of chitosan and an ionic liquid. The electrochemical performance of the biosensor was investigated by cyclic voltammetry and the relative parameters have been optimized by amperometric measurements in pH 5.17 acetate buffer solution. The developed biosensor exhibited a linear range for glucose between 0.1–1.0 mM and the limit of detection was calculated as 0.02 mM.
A Novel Strategy for Quantifying Clopidogrel Using Square‐wave Voltammetry and a Boron‐doped Diamond Film
Authors: Nascimento Louise Oliveira , Scremin Jessica , Mattos Gabriel Junquetti , Gomes Adriana , Clausen Débora Nobile , Sartori Elen Romão 
Published: September 2019

AbstractThe voltammetric behavior of clopidogrel bisulfate (CLO), an antiplatelet agent, was investigated for the first time in the literature on a cathodically pretreated boron‐doped diamond electrode (CP‐BDDE) using cyclic (CV) and square‐wave voltammetry (SWV). It was observed an anodic peak for CLO, suitable for analytical purposes, at about 1.15 V (vs. Ag/AgCl (3.0 mol L−1 KCl)) by CV in Britton‐Robinson buffer solution (pH 5.0). On the physical‐chemical characterization of the interface phenomena, it was proved that electrode reaction of the analyte was controlled by a diffusion process. At optimized square‐wave parameters (pulse amplitude of 60 mV, frequency of 30 Hz and scan increment of 3 mV), the obtained analytical curve was linear for the CLO concentration range from 0.60 to 60.0 μmol L−1, with a detection limit of 0.60 μmol L−1. The simple, rapid and greener analytical method, based on CP‐BDDE electrochemical sensor, was successfully applied in real samples (pharmaceuticals and urine).