bluuebunny/crossref_metadata_2025_split huggingface dataset:
The long noncoding RNA TPTE2P1 promotes the viability of colorectal cancer cells
Authors: Dai Xiaoyu , Xie Yangyang , Dong Mingjun , Zhao Jianpei , Yu Hua , Zhou Baofeng , Xu Yidong , Yu Yongming , Cao Yisheng , Zhang Youping 
Published: November 2018

AbstractLong noncoding RNAs (lncRNAs) have important functions in tumor development and progression, including colorectal cancer (CRC), but their roles are not completely understood. In this study, the roles of the lncRNA transmembrane phosphoinositide 3‐phosphatase and tensin homolog 2 pseudogene 1 (TPTE2P1), previously implicated in gallbladder cancer cell migration and invasion, were evaluated in CRC. In particular, quantitative polymerase chain reaction was used to quantify TPTE2P1 levels in tumor tissues and cell lines. The association between TPTE2P1 and survival was analyzed using the online tool OncoLnc. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assays, colony formation assays, and flow cytometry were used to evaluate the effects of TPTE2P1 on viability, cell cycle progression, and apoptosis. Signaling pathway proteins were quantitated by Western blot analysis. Finally, the role of TPTE2P1 was analyzed in vivo using mouse models. TPTE2P1 levels were significantly higher in CRC tissues than in adjacent normal tissues. Higher expression was associated with a poor survival rate. The silencing of TPTE2P1 led to cell cycle arrest at the S phase and thereby inhibited cell viability. TPTE2P1 knockdown also caused cancer cell apoptosis via the activation of the apoptosis regulator (BCL2)/caspase 3 signaling cascade. In addition, the inhibition of TPTE2P1 had suppressive effects on tumors in vivo. TPTE2P1 is upregulated in CRC and plays essential roles in the regulation of cell viability in vitro and tumor formation in vivo.
The impact of interferon‐free direct‐acting antivirals on clinical outcome after curative treatment for hepatitis C virus–associated hepatocellular carcinoma: Comparison with interferon‐based therapy
Authors: Nagaoki Yuko , Imamura Michio , Nishida Yuno , Daijo Kana , Teraoka Yuji , Honda Fumi , Nakamura Yuki , Morio Kei , Fujino Hatsue , Nakahara Takashi , Kawaoka Tomokazu , Tsuge Masataka , Hiramatsu Akira , Kawakami Yoshiiku , Miki Daiki , Hiyama Yuichi , Ochi Hidenori , Chayama Kazuaki , Aikata Hiroshi 
Published: November 2018

AbstractBackground and AimTo examine the effect on recurrence and survival of treatment by interferon (IFN)‐free direct‐acting antivirals (DAA) for patients with hepatitis C virus (HCV)‐associated hepatocellular carcinoma (HCC) who underwent primary curative treatment.MethodsThis was a retrospective cohort study of 250 patients with HCV who had received curative treatment for primary HCC. As anti‐HCV treatment after HCC treatment, 38 patients received IFN‐free DAA therapy (DAA patients) and 94 received IFN‐based therapy (IFN patients). The recurrence of HCC and overall survival of the patient groups were compared in a case‐control study.ResultsThe cumulative HCC recurrence rates at 1, 3, and 5 years were 5%, 39%, and 39% for DAA patients and 0%, 46%, and 62% for IFN patients, respectively (P = 0.370). Multivariate analysis of the HCC recurrence identified treatment responses (sustained virological response [SVR]: hazard ratio [HR] 2.237;
P = 0.003) as an independent predictive factor. The cumulative overall survival rates at 3 and 5 years were 96%, 96% for DAA patients and 93%, 73% for IFN patients, respectively (
P = 0.163). Multivariate analysis identified treatment responses (SVR: HR 8.742;
P < 0.001) as independent predictors of overall survival. Propensity score matching analysis showed no significant difference in HCC development rates and overall survival rates in the two groups.ConclusionsWe found that SVR obtained after curative treatment for primary HCC suppressed recurrence and improved overall survival. And, IFN‐free DAA therapy after curative treatment for primary HCC could predict improving overall survival and suppressed HCC recurrence.
p107 Deficiency Increases Energy Expenditure by Inducing Brown‐Fat Thermogenesis and Browning of White Adipose Tissue
Authors: Cunarro Juan , Buque Xabier , Casado Sabela , Lugilde Javier , Vidal Anxo , Mora Alfonso , Sabio Guadalupe , Nogueiras Rubén , Aspichueta Patricia , Diéguez Carlos , Tovar Sulay 
Published: November 2018

ScopeThe tumor suppressor p107, a pocket protein member of the retinoblastoma susceptibility protein family, plays an important role in the cell cycle and cellular adipocyte differentiation. Nonetheless, the mechanism by which it influences whole body Energy homeostasis is unknown.Methods and ResultsThe phenotype of p107 knockout (KO) mixed‐background C57BL6/129 mice phenotype is studied by focusing on the involvement of white and brown adipose tissue (WAT and BAT) in energy metabolism. It is shown that p107 KO mice are leaner and have high‐fat diet resistence. This phenomenon is explained by an increase of energy expenditure. The higher energy expenditure is caused by the activation of thermogenesis and may be mediated by both BAT and the browning of WAT. Consequently, it leads to the resistance of p107 KO mice to high‐fat diet effects, prevention of liver steatosis, and improvement of the lipid profile and glucose homeostasis.ConclusionThese data allowed the unmasking of a mechanism by which a KO of p107 prevents diet‐induced obesity by increasing energy expenditure via increased thermogenesis in BAT and browning of WAT, indicating the relevance of p107 as a modulator of metabolic activity of both brown and white adipocytes. Therefore, it can be targeted for the development of new therapies to ameliorate the metabolic syndrome.
Degenerative chain‐transfer process: Controlling all chain‐growth polymerizations and enabling novel monomer sequences
Authors: Kamigaito Masami , Satoh Kotaro , Uchiyama Mineto 
Published: November 2018

ABSTRACTThe use of dormant species has opened a new era in precision polymerization and has changed the concept of living polymerization. The dormant species can be exchanged into the active species via reversible termination or via reversible chain transfer. Professor Mitsuo Sawamoto has greatly contributed to the establishment of the concepts of living cationic and radical polymerizations based on the reversible activation of dormant species. This highlight, dedicated to Professor Sawamoto on his retirement from Kyoto University, provides an overview of reversible or degenerative chain‐transfer (DT) processes, which are effective in controlling all chain‐growth polymerizations, including radical, cationic, anionic, coordination, ring‐opening metathesis, and ring‐opening polymerizations. In addition, structures with novel sequences accessible only by a combination of different propagating species with a common DT agent are reviewed. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2019, 57, 243–254
Cross‐strand disulfides in the hydrogen bonding site of antiparallel β‐sheet (aCSDhs): Forbidden disulfides that are highly strained, easily broken
Authors: Haworth Naomi L. , Wouters Michael J. , Hunter Morgan O. , Ma Lixia , Wouters Merridee A. 
Published: November 2018

AbstractSome disulfide bonds perform important structural roles in proteins, but another group has functional roles via redox reactions. Forbidden disulfides are stressed disulfides found in recognizable protein contexts, which currently constitute more than 10% of all disulfides in the PDB. They likely have functional redox roles and constitute a major subset of all redox‐active disulfides. The torsional strain of forbidden disulfides is typically higher than for structural disulfides, but not so high as to render them immediately susceptible to reduction under physionormal conditions. Previously we characterized the most abundant forbidden disulfide in the Protein Data Bank, the aCSDn: a canonical motif in which disulfide‐bonded cysteine residues are positioned directly opposite each other on adjacent anti‐parallel β‐strands such that the backbone hydrogen‐bonded moieties are directed away from each other. Here we perform a similar analysis for the aCSDh, a less common motif in which the opposed cysteine residues are backbone hydrogen bonded. Oxidation of two Cys in this context places significant strain on the protein system, with the β‐chains tilting toward each other to allow disulfide formation. Only left‐handed aCSDh conformations are compatible with the inherent right‐handed twist of β‐sheets. aCSDhs tend to be more highly strained than aCSDns, particularly when both hydrogen bonds are formed. We discuss characterized roles of aCSDh motifs in proteins of the dataset, which include catalytic disulfides in ribonucleotide reductase and ahpC peroxidase as well as a redox‐active disulfide in P1 lysozyme, involved in a major conformation change. The dataset also includes many binding proteins.
Residence Life, Men, and Masculinities: Recruitment, Training, and Administrative Processes
Authors: Wagner Rachel , Tillapaugh Daniel 
Published: November 2018

AbstractAuthors examine how interrogating hegemonic gender roles can enhance recruitment, training, and engagement practices for residence life staff in leadership positions. Under specific focus are issues of gendered expectations of the men as paraprofessional role, dynamics in supervision, recruitment and training of staff, and bystander intervention in the residential community.
Treatment of fetal circular shunt with non‐steroidal anti‐inflammatory drugs
Authors: Torigoe T. , Mawad W. , Seed M. , Ryan G. , Marini D. , Golding F. , VAN Mieghem T. , Jaeggi E. 
Published: November 2018

ABSTRACTA circular shunt (CS) is a life‐threatening condition involving massive shunting of systemic arterial blood via the ductus arteriosus to the left ventricle without traversing the lungs. In the prenatal setting, it occurs mainly in fetuses with severe forms of Ebstein's anomaly (EA) owing to unrestricted ductal flow and significant pulmonary and tricuspid regurgitation. We aimed to improve the fetal hemodynamics and chances of survival of affected fetuses by inducing ductal constriction using transplacental non‐steroidal anti‐inflammatory drugs (NSAIDs). Following initiation of treatment between 26 and 34 weeks' gestation, three (75%) of four fetuses with EA/CS responded with sustained ductal constriction and improved hemodynamic function, which allowed continuation of pregnancy for 3–7 weeks and elective delivery. All successfully treated cases underwent neonatal surgery immediately after birth to eliminate the CS and survived. This included two neonates that underwent single‐ventricle palliation surgery that required postoperative extracorporeal membrane oxygenation and hemofiltration for transient respiratory and renal failure. The one case that did not respond to treatment with NSAIDs was delivered prematurely for progressive fetal compromise and died shortly after birth. Transplacental treatment with NSAIDs represents a novel approach to controlling fetal CS, avoiding in‐utero death and prolonging the pregnancy to a more advanced gestational age, thereby potentially increasing the chances of neonatal survival. This treatment should be considered and initiated at an early stage of systemic steal to prevent brain injury due to hypoperfusion. Copyright © 2018 ISUOG. Published by John Wiley & Sons Ltd.
Increased Sylvian fissure angle as early sonographic sign of malformation of cortical development
Authors: Pooh R. K. , Machida M. , Nakamura T. , Uenishi K. , Chiyo H. , Itoh K. , Yoshimatsu J. , Ueda H. , Ogo K. , Chaemsaithong P. , Poon L. C. 
Published: November 2018

ABSTRACTObjectiveTo evaluate Sylvian fissure development by assessing Sylvian fissure angles in fetuses with malformation of cortical development (MCD).MethodsThis was a retrospective study of 22 fetuses with MCD. Cases with a stored three‐dimensional (3D) brain volume acquired at 18 + 0 to 30 + 6 weeks of gestation at an ultrasound‐based research clinic between January 2010 and December 2017 were identified through a database. Of the 22 fetuses, seven had an extracranial abnormality, such as cardiac, renal, gastrointestinal and/or digital anomalies, and five had a minor abnormality such as micrognathia, low‐set ears and/or single umbilical artery. To confirm the final clinical diagnosis of brain abnormality, postmortem histological findings or prenatal or postnatal magnetic resonance images were used. For measurement of Sylvian fissure angle, an anterior coronal plane of the fetal brain on transvaginal 3D volume multiplanar imaging was visualized as a single image from the three orthogonal views. The right and left Sylvian fissure angles were measured between a horizontal reference line (0°) and a line drawn along the upper side of the respective Sylvian fissure. The Sylvian fissure angle on both sides was plotted on the graphs of the reference ranges for gestational age in weeks.ResultsIn 21 (95.5%; 95% CI, 86.8–100.0%) of 22 fetuses with MCD, the Sylvian fissure angle on one or both sides was larger than the 90th percentile of the normal reference. There was one case with apparent focal MCD in the parietal lobe, but the Sylvian fissure angles were normal. A case with apparent unilateral cortical dysplasia and one with apparent unilateral schizencephaly had conspicuous discrepancies between the left and right Sylvian fissure angles. Abnormal genetic test results were obtained in six cases, including four cases with a mutation in a single gene.ConclusionsThis study has shown that the Sylvian fissures, as defined by the Sylvian fissure angle, have delayed development in most MCD cases prior to the diagnosis of the condition. The Sylvian fissure angle may potentially be a strong indicator for the subsequent development of cortical malformation, before the time point at which the gyri and sulci become obvious on the fetal brain surface. Further research is required to validate these findings. © 2018 The Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of the International Society of Ultrasound in Obstetrics and Gynecology.
The relationships between precipitation amounts, number of rain days, and relative vorticity in the mid‐troposphere over Iran
Authors: Darand Mohammad , Mirzaei Nabi 
Published: November 2018

AbstractPrecipitation is one of the most studied atmospheric parameters, and its occurrence has a direct relationship with the atmospheric circulation pattern. This study investigates the relationship between relative vorticity in the mid‐troposphere (at 500 hPa), and precipitation amounts and the number of rain days over the period 1979–2013 in Iran. Relative vorticity was calculated using the u and v components of the wind data from the European Centre for Medium‐Range Weather Forecasts (ECMWF) interim reanalysis (ERA‐Interim) over Iran, with 0.125° spatial resolution. Gridded daily precipitation data from the Asfazari National Dataset with a spatial resolution of 15 × 15 km, covering the same period, were also used. Pearson's correlation coefficient was used to investigate, at the 90% confidence level, the relationship between relative vorticity, and monthly precipitation amount and number of rain days. The significance of the trend for relative vorticity for different months, at the 90% confidence level, was evaluated using the nonparametric modified Mann–Kendall test. The results showed a significant relationship between relative vorticity and the number of rain days and precipitation amounts over Iran. In temporal terms, the correlations between relative vorticity and the number of rain days and precipitation amounts are strongest during the months of high precipitation over Iran, especially in November and December. In spatial terms, eastern Iran exhibits the strongest relationship between precipitation and relative vorticity at the 500 hPa level. Examination of the time series data showed that the monthly precipitation amounts and numbers of rain days fluctuate in line with changes in relative vorticity during the period of study. Analysis of trends in the relative vorticity data also showed, at the 90% confidence level, that the maximum variations in relative vorticity occurred in autumn and winter, respectively. Trend analysis also showed a decreasing trend in relative vorticity in March and an increasing trend in November.
Analytic results for the planar double box integral relevant to top-pair production with a closed top loop
Authors: Adams Luise , Chaubey Ekta , Weinzierl Stefan 
Published: November 2018

AbstractIn this article we give the details on the analytic calculation of the master integrals for the planar double box integral relevant to top-pair production with a closed top loop. We show that these integrals can be computed systematically to all order in the dimensional regularisation parameterε. This is done by transforming the system of differential equations into a form linear inε, where theε0-part is a strictly lower triangular matrix. Explicit results in terms of iterated integrals are presented for the terms relevant to NNLO calculations.
In‐Country Training by the Ghana College of Physicians and Surgeons: An Initiative that has Aided Surgeon Retention and Distribution in Ghana
Authors: Gyedu Adam , Debrah Sam , Agbedinu Kwabena , Goodman Stephanie K. , Plange‐Rhule Jacob , Donkor Peter , Mock Charles 
Published: November 2018

AbstractBackgroundPrior to 2003, production of new surgeons in Ghana was limited. In 2003, the Ghana College of Physicians and Surgeons (GCPS) initiated the first wholly in‐country training and credentialing of surgeons. The purpose of this study was to assess the impact of in‐country training of surgeons in Ghana.MethodsWe interviewed 117 (80%) of the 146 surgeons trained through the GCPS from inception through 2016. We gathered data on type of training, practice location, clinical workload, and administrative and teaching roles. Operations were categorized into those deemed essential (most cost‐effective, highest population impact) by the World Bank’s Disease Control Priorities project versus other.ResultsIn‐country retention was 87–97%. A little more than half (56%) were working in the two largest cities and 44% were working in higher need areas. Twenty‐two (19%) were the first surgeon to have worked at their current hospital. The surgeons performed a mean of 13 operations per week (seven electives, six emergencies). 35% of elective and 77% of emergency operations were in the essential category. Most (79%) surgeons were engaged in training/teaching; 46% were engaged in research; and 33% held an administrative office.ConclusionsIn‐country surgical training has led to high retention and wide geographic distribution, including high need areas. The in‐country trained surgeons are playing key roles in clinical practice, training, and administration. These data provide support for investments in similar efforts in other low‐ and middle‐income countries.
Internet-delivered cognitive behaviour therapy for chronic health conditions: a systematic review and meta-analysis
Authors: Mehta Swati , Peynenburg Vanessa A. , Hadjistavropoulos Heather D. 
Published: November 2018

AbstractThis systematic review and meta-analysis aims to evaluate the effectiveness of internet-delivered cognitive behavioural therapy (ICBT) on anxiety and depression among persons with chronic health conditions. A systematic database search was conducted of MEDLINE, CINAHL, PsycInfo, EMBASE, and Cochrane for relevant studies published from 1990 to September 2018. A study was included if the following criteria were met: (1) randomized controlled trial involving an ICBT intervention; (2) participants experienced a chronic health condition; (3) participants ≥ 18 years of age; and (4) effects of ICBT on anxiety and/or depression were reported. The Cochrane Risk of Bias tool was used to assess the risk of bias on the included studies. Pooled analysis was conducted on the primary and condition specific secondary outcomes. Twenty-five studies met inclusion criteria and investigated the following chronic health conditions: tinnitus (n = 6), fibromyalgia (n = 3), pain (n = 7), rheumatoid arthritis (n = 3), cardiovascular disease (n = 2), diabetes (n = 1), cancer (n = 1), heterogeneous chronic disease population (n = 1), and spinal cord injury (n = 1). Pooled analysis demonstrated small effects of ICBT in improving anxiety and depression. Moderate effects of therapist-guided approach were seen for depression and anxiety outcomes; while, self-guided approaches resulted in small effects for depression and moderate effects in anxiety outcomes. ICBT shows promise as an alternative to traditional face-to-face interventions among persons with chronic health conditions. Future research on long-term effects of ICBT for individuals with chronic health conditions is needed.Trial RegistrationPROSPERO registration number: CRD42018087292.
Improvement in Residents’ Attitudes Toward Individuals with Substance Use Disorders Following an Online Training Module on Stigma
Authors: Avery Jonathan , Knoepflmacher Daniel , Mauer Elizabeth , Kast Kristopher A. , Greiner Miranda , Avery Joseph , Penzner Julie B. 
Published: November 2018

Background Resident physicians have been shown to possess negative attitudes toward individuals with substance use disorders (SUDs), even if the residents believe they have adequate knowledge and skills to care for these patients. Residents’ negative attitudes may have an adverse impact on patient engagement, treatment, and outcomes. Questions/Purposes The goal of this study was to examine the impact of an online training module on residents’ attitudes toward people with SUDs. We hypothesized that residents who received the educational intervention would show improved attitudes toward people with alcohol and opioid use disorders. Methods A web-based questionnaire, including demographic information and the Medical Condition Regard Scale (MCRS) about individuals with alcohol and opioid use disorders, was sent to internal medicine and psychiatry residents before and 6 months after they took an online training module on stigma toward individuals with SUDs. Results A total of 46 residents completed the initial questionnaire and 29 completed the follow-up questionnaire 6 months later. Attitudes toward individuals with SUDs, as reflected by an increase in MCRS scores, were improved 6 months after the online training module. Conclusion Residents’ attitudes toward individuals with SUDs improved after taking an online training module. This is encouraging, as studies have shown that attitudes toward individuals with SUDs tend to decline during residency training and negatively affect patient care. Larger studies are needed to determine if such online modules can improve attitudes of other groups of clinicians, result in sustained change over time, and improve patient outcomes.