bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in plasma biomarkers differentiate clinical stages of Alzheimer's disease using immunomagnetic reduction assays
Authors: Chao Shu-Ping , Lin Wei-Che , Lu Cheng-Hsien , Wu Hsiu-Chuan , Liu Hsing-Cheng , Hu Chaur-Jong 
Published: December 2024

Background Many groups have been using immunomagnetic reduction (IMR) for assaying plasma amyloid-β 1-40 (Aβ1-40) peptide, Aβ1-42 peptide and total tau protein (T-Tau) in cognitively normal controls (NC), patients with amnestic mild cognitive impairment (aMCI) and Alzheimer's disease dementia (ADD). Tremendous results have been independently reported. Objective We used traditional knowledge databases (e.g., PubMed, Embase), papers published at international conferences, and private communications to obtain data involving the use of IMR assay for plasma biomarkers of plasma Aβ1-40, Aβ1-42, and T-Tau in NC, aMCI, and ADD. Methods Results of thirty studies were analyzed, including twenty-five studies published in papers, two studies presented at conferences and three unpublished studies. Results Among the thirty studies, there were 1189 subjects of NC, 544 aMCI patients and 853 ADD patients. The average value of plasma Aβ1-40 in subjects significantly decreased from NC (59.72 pg/ml) to aMCI (45.92 pg/ml, p < 0.0001), which was no significant different from ADD (48.34 pg/ml, p > 0.05). The average level of plasma Aβ1-42 significantly increased from NC (15.72 pg/ml) to aMCI (17.66 pg/ml, p < 0.0001), which was not significantly different from ADD (19.39 pg/ml, p > 0.05). However, the average level of plasma T-Tau significantly increased from NC (17.92 pg/ml) to aMCI (28.26 pg/ml, p < 0.0001), further significantly increased to AD (35.51 pg/ml, p < 0.001). Conclusions Plasma Aβ1-40, Aβ1-42, and T-Tau levels are able to discriminate NC from patients with aMCI and ADD. Plasma T-Tau levels are disease-severity dependent.
Sensitivity and Specificity of Modified Early Obstetric Warning Score (MEOWS) and Maternal Early Warning Criteria (MEWC) for Maternal Morbidity: A Retrospective Cohort Study in Pregnant Women with COVID-19
Authors: Cahyani Dinda Desti , Priyanto Heru , Dhiya An Alfun , Supriyatiningsih  
Published: December 2024

Objective: To measure the comparative sensitivity, specificity, and predictive value of MEOWS and MEWC in predicting morbidity in pregnant patients infected with COVID-19. This research can be one of the screenings used to determine the level of care for pregnant patients with COVID-19.
Methods: The retrospective cohort technique was used to examine the 89 pregnant women with COVID-19 who were admitted to the Bantul Regional General Hospital between January and December 2021. Data analysis used the ROC curve to compare sensitivity, specificity, and predictive value.
Results: MEWC is better than MEOWS in predicting the morbidity of pregnant patients with COVID-19 infection. This is because MEWC has a better sensitivity (78,3%) and PPV value (78%) than MEOWS, even though it has a lower specificity value (81,8%) and NPV value (82%). The specificity value of MEWC (81.8%), when compared to MEOWS (97.1%), does have a lower specificity value. The results of MEOWS and MEWC data analysis using ROC produce an area under the curve for MEOWS of 74,9% while for MEWC of 80%.
Discussion: MEWC has a better sensitivity indicating that patients who do not trigger MEWC criteria will have a low risk of experiencing maternal morbidity. Screening tools will prioritize the sensitivity value compared to the specificity value of the instrument used. A screening tool will have a lower positive predictive value if the study population has a lower prevalence of morbidity. Based on the comparison of the predictive value, sensitivity, and specificity of the MEWC and MEOWS instruments, it can be concluded that MEWC is associated with maternal morbidity with a higher sensitivity than MEOWS, although it has a lower specificity. High sensitivity values will result in screening tools with consistent results. The ROC curve can also show that MEWC has a higher sensitivity value by looking at the Y-coordinate, which is higher than the Y-coordinate of MEOWS. MEWC has a better Receiver Operator Curve (ROC) intersection point than MEOWS, where the MEWC intersection point has the furthest point on the upper left of the ROC diagonal line.
Conclusion: MEWC has a higher sensitivity compared to MEOWS, even though it has a lower specificity. High sensitivity values will produce screening tools with consistent results.
Keywords: MEWC, maternal early warning system, maternal morbidity, MEOWS
Peningkatan Minat Belajar Bahasa Inggris melalui Metode Small Group Discussion pada SMP IT Qurrata A’yun Kota Palu
Authors: Jamiluddin Jamiluddin , Zamzam Fadhilah , Yatun Anasthasia Woluta , Nirwana Nirwana 
Published: December 2024

Kegiatan pengabdian ini dilatarbelakangi adanya salah satu hasil penelitian tentang penguasaan bahasa Inggris di Indonesia yang masih rendah dan belum memberikan harapan yang baik. Data menunjukkan bahwa materi grammar bahasa Inggris paling membingungkan, diikuti oleh materi berbicara (speaking) sulit dipelajari. Dengan demikian tujuan dilakukan pengabdian ini adalah Peningkatan Minat Belajar Bahasa Inggris melalui Metode Small Group Discussion Siswa SMP IT Qurrota A’yun, Palu. Tahapan pelaksanaannya melalui metode pendekatan pembelajaran berupa kegiatan edukasi terhadap proses pembelajaran bahasa Inggris yang merujuk pada proses belajar bersama dalam bentuk ‘Small Group Discussion’. Hasil pengabdian yang telah dilakukan dalam bentuk pelatihan menunjukkan bahwa model pembelajaran ‘small group discussion’ membuat semua peserta didik aktif dalam kegiatan belajar mengajar, mengajarkan kepada peserta didik menghargai pendapat orang lain dan bekerjasama dengan teman yang lain, melatih dan mengembangkan sikap sosial dan demokratis siswa, meningkatkan keterampilan berkomunikasi bagi siswa, mempertinggi partisipasi peserta didik baik secara individual dalam kelompok maupun dalam kelas, dan mengembangkan pengetahuan mereka, karena saling bertukar pendapat antar siswa dalam kelompoknya.
Urinary microbiome profiling as a non-invasive tool for identifying biomarkers in systemic lupus erythematosus and lupus nephritis
Authors: Shi Bo , Chen Fei , Gong Jianmin , Khan Adeel , Qian Xiang , Xu Zhipeng , Yang Ping 
Published: December 2024

IntroductionBacteriome alterations have been implicated in the pathogenesis of systemic lupus erythematosus (SLE). However, the relationship between SLE and the urinary microbiome remains underexplored. This study aimed to characterize the urinary microbiome of SLE patients using 16S rRNA sequencing and to investigate its correlations with clinical parameters through integrative analyses.MethodsUrine sediment samples were collected from individuals with SLE and lupus nephritis (LN) (n = 20), SLE without LN (n = 22), and healthy controls (HCs) (n = 23). DNA was extracted and subjected to 16S rRNA sequencing to profile the urinary microbiome. Receiver operating characteristic (ROC) curve analysis was conducted to evaluate the diagnostic efficacy of urinary microbiota, while Spearman’s correlation analysis was employed to identify links between specific microbial taxa and clinical parameters. Functional predictions of bacterial roles were performed using Picrust2.ResultsThe urinary microbiota diagnostic model exhibited excellent performance in distinguishing SLE patients from HCs. Spearman’s analysis revealed significant correlations between the urinary microbiome and clinical parameters. Specifically, Sphingomonas and Lachnospiraceae genera showed positive correlations with vitamin D levels, cylinderuria, and proteinuria, while Pedobacter, Aquabacterium, Delftia, and Achromobacter displayed negative correlations with proteinuria and albumin-to-creatinine ratio (ACR). Functional predictions indicated that the urinary microbiome might influence immune regulation through modulation of signaling pathways and metabolic processes.DiscussionOur study is the first to reveal dysbiosis in the urinary microbiome of patients with SLE. Certain bacterial taxa in the urinary microbiome were identified as potential diagnostic biomarkers for SLE. Furthermore, the functional implications of these bacterial communities suggest their involvement in immune modulation, highlighting the potential for further investigation into their roles in SLE pathogenesis and diagnosis.
Human airway epithelium controls Pseudomonas aeruginosa infection via inducible nitric oxide synthase
Authors: Grubwieser Philipp , Böck Nina , Soto Erika Kvalem , Hilbe Richard , Moser Patrizia , Seifert Markus , Dichtl Stefanie , Govrins Miriam Alisa , Posch Wilfried , Sonnweber Thomas , Nairz Manfred , Theurl Igor , Trajanoski Zlatko , Weiss Günter 
Published: December 2024

IntroductionAirway epithelial cells play a central role in the innate immune response to invading bacteria, yet adequate human infection models are lacking.MethodsWe utilized mucociliary-differentiated human airway organoids with direct access to the apical side of epithelial cells to model the initial phase of Pseudomonas aeruginosa respiratory tract infection.ResultsImmunofluorescence of infected organoids revealed that Pseudomonas aeruginosa invades the epithelial barrier and subsequently proliferates within the epithelial space. RNA sequencing analysis demonstrated that Pseudomonas infection stimulated innate antimicrobial immune responses, but specifically enhanced the expression of genes of the nitric oxide metabolic pathway. We demonstrated that activation of inducible nitric oxide synthase (iNOS) in airway organoids exposed bacteria to nitrosative stress, effectively inhibiting intra-epithelial pathogen proliferation. Pharmacological inhibition of iNOS resulted in expansion of bacterial proliferation whereas a NO producing drug reduced bacterial numbers. iNOS expression was mainly localized to ciliated epithelial cells of infected airway organoids, which was confirmed in primary human lung tissue during Pseudomonas pneumonia.DiscussionOur findings highlight the critical role of epithelial-derived iNOS in host defence against Pseudomonas aeruginosa infection. Furthermore, we describe a human tissue model that accurately mimics the airway epithelium, providing a valuable framework for systemically studying host-pathogen interactions in respiratory infections.
Genetic diversity and population structure analysis of 418 tomato cultivars based on single nucleotide polymorphism markers
Authors: Xu Weijie , Gong Chao , Mai Peiting , Li Zhenxing , Sun Baojuan , Li Tao 
Published: December 2024

IntroductionTomato (Solanum lycopersicum) is a highly valuable fruit crop. However, due to the lack of scientific and accurate variety identification methods and unified national standards, production management is scattered and non-standard, resulting in mixed varieties. This poses considerable difficulties for the cataloging and preservation of germplasm resources as well as the identification, promotion, and application of new tomato varieties.MethodsTo better understand the genetic diversity and population structure of representative tomato varieties, we collected 418 tomato varieties from the past 20 years and analyzed them using genome-wide single nucleotide polymorphism (SNP) markers. We initially assessed the population structure, genetic relationships, and genetic profiles of the 418 tomato germplasm resources utilizing simplified genome sequencing techniques. A total of 3,374,929 filtered SNPs were obtained and distributed across 12 chromosomes. Based on these SNP loci, the 418 tomatoes samples were divided into six subgroups.ResultsThe population structure and genetic relationships among existing tomato germplasm resources were determined using principal component analysis, population structure analysis, and phylogenetic tree analysis. Rigorous selection criteria identified 15 additional high-quality DNA fingerprints from 50 validated SNP loci, effectively enabling the identification of the 418 tomato varieties, which were successfully converted into KASP (Kompetitive Allele Specific PCR) markers.DiscussionThis study represents the first comprehensive investigation assessing the diversity and population structure of a large collection of tomato varieties. Overall, it marks a considerable advancement in understanding the genetic makeup of tomato populations. The results broadened our understanding of the diversity, phylogeny, and population structure of tomato germplasm resources. Furthermore, this study provides a scientific basis and reference data for future analysis of genetic diversity, species identification, property rights disputes, and molecular breeding in tomatoes.
Genome-wide identification and expression analysis of the AS2/LOB gene family in physic nut
Authors: Tang Yuehui , Wang Xiaohui , Feng Jiayu , Wang Yaoyao , Liu Tengfei , Bao Xinxin 
Published: December 2024

IntroductionAS2/LOB genes, a class of transcription factors ubiquitously existing in plants, are vital for plant growth, development, and stress tolerance. Despite the availability of the physic nut genome, information regarding the expression profiles and evolutionary histories of its AS2/LOB genes remains scarce.MethodsAn elaborate exploration of the AS2/LOB gene family was conducted, including phylogeny, exon-intron structure, chromosomal location, conserved domain characteristics, conserved motifs, promoter cis-acting elements, protein interaction, and expression profiles under normal growth and abiotic stress conditions.ResultsIn this study, 28 AS2/LOB genes (JcASLs) were identified in the physic nut genome. Phylogenetic analysis, based on homologs from Arabidopsis, classified the 28 JcASLs genes into two groups (calss I and II). Chromosome localization indicated that the 28 JcASLs genes were unevenly distributed across nine chromosomes. RNA-seq and qRT-PCR results revealed that the majority of the 28 JcASLs genes exhibited differential expression in tissues such as roots, cortex stems, leaves, and seeds. Notably, JcASL8 and JcASL13 were exclusively expressed in seeds, and 16 JcASLs genes responded to drought and salt stress at least at one time point under at least one treatment condition.DiscussionThese results establish a basis for future investigations into the molecular mechanism by which the JcASLs genes regulate physic nut's response to drought and salt stress and their role in modulating the growth and development of physic nut.
Food waste and its associated environmental impacts in workplace buffet-style canteens in Beijing, China
Authors: Zhang Dan , Zhang Panpan , Xue Li , Wu Liang 
Published: December 2024

IntroductionFood waste is a significant global challenge, with critical implications for food security, ecosystem services, biodiversity, and human health. Despite growing attention to food waste in food service sectors, workplace buffet-style canteens—particularly in China—remain understudied. This research addresses this gap by quantifying food waste and evaluating its environmental impacts in workplace buffet-style canteens in Beijing, China.MethodsField surveys were conducted in four workplace buffet-style canteens in Beijing during August and September 2021. Systematic sampling yielded 656 valid samples. Food waste was measured using a direct weighing method, categorized into eight food types, and analyzed for its environmental impacts using carbon, nitrogen, phosphorus, water, and ecological footprint metrics. Conversion factors were applied to translate cooked food into raw agricultural equivalents for consistent impact assessment.ResultsThe study found an average daily per capita food waste of 158.4 g, with lunch contributing the highest waste (78.8 g) compared to breakfast and dinner. While vegetables were the largest contributors by weight, livestock products, though comprising only 29.9% of waste, accounted for the majority of environmental impacts, including 96.7% of the ecological footprint and 85.7% of the water footprint. Self-run canteens generated less waste across all meals compared to outsourced ones, and higher food and cuisine diversity were associated with increased food waste.DiscussionThe findings underscore the influence of dietary habits, management models, and food diversity on food waste generation. Livestock product waste poses disproportionate environmental burdens, highlighting the need for targeted interventions such as portion control and educational campaigns on sustainable dining practices. These results provide valuable insights for policymakers aiming to align food waste reduction strategies with Sustainable Development Goal 12.3.
Бразилия во внешнеполитической паутине Трампа
Authors: БАЖЕНОВА О.А. 
Published: December 2024

В 1986 г. в американском политическом лексиконе появился новый термин основной союзник вне НАТО (ОСВН). Он обозначает особый статус, присваиваемый главой Белого дома странам, не входящим в Североатлантический альянс, с которыми у США складываются тесные отношения в военно-стратегической сфере. Летом 2019 г. ряды обладателей статуса ОСВН пополнила Бразилия. Цель статьи изучить роль латиноамериканского государства во внешнеполитической стратегии Д. Трампа.
In 1986 the American political dictionary has been enriched with a new concept Major Non-NATO Ally (MNNA). This term defines a specific status attributed by the US president to countries beyond the North Atlantic Alliance with whom the United States develops close military-strategic relationship. In2019 Brazil joined MNNA ranks. Article studies Latin American states role in Trumps foreign strategy.
Методика типологического анализа управленческой культуры офицера в воинском подразделении ВС РФ
Authors: ЕРОХИН Е.Г. 
Published: December 2024

Статья раскрывает логику проведения типологического анализа управленческой культуры офицера в воинском подразделении Вооруженных Сил Российской Федерации.
The article reveals the logic of carrying out a typological analysis of officers management culture in the military unit of the Armed Forces of the Russian Federation.
К вопросу о социальном управлении служебным поведением военнослужащих Вооруженных Сил Российской Федерации
Authors: ПОГРЕБНОЙ Д.Н. 
Published: December 2024

В статье дается аналитический обзор специфики социального управления служебным поведением военнослужащих. Рассмотрены различные аспекты управления с точки зрения их социальной роли и места в системе управления служебным поведением.
In article the analytical review of specificity of social management of service behavior of military men is given. Different aspects of management from the point of view of their social role and place in the system of management of official behavior are considered.
Pengaruh Model Two Stay Two Stray Melalui Diagram Fishbone Terhadap Keterampilan Menulis Siswa Kelas V SD Negeri 3 Puding Besar
Authors: Kamelia Husna  , Maulina Hendrik  , Silvio Juliana Nabela  
Published: December 2024

Penelitian ini dilatarbelakangi oleh kurangnya keterampilan menulis siswa dalam menulis. Kesulitan yang dialami peserta didik yaitu: kesulitan dalam penggunaan ejaan dan tanda baca, penulisan huruf kapital yang kata kurang tepat serta kerapian tulisan yang belum sesuai dengan aspek penilaian keterampilan menulis. Penelitian ini bertujuan untuk mengetahui pengaruh model two stay two stray melalui diagram fishbone terhadap keterampilan menulis kelas V SD Negeri 3 Puding Besar.
Penelitian ini menggunakan metode penelitian kuantitatif. desain yang digunakan dalam penelitian ini adalah desain eksperimen. Desain yang digunakan dalam penelitian ini adalah pre-experimental design dalam bentuk one group pretest-posttest design. Teknik dalam pengambilan sampel pada penelitian ini menggunakan sampling jenuh. Teknik pengumpulan data dalam penelitian ini berupa tes, yaitu pretest dan posttest. Teknik analisis data yang digunakan adalah Uji Paired Sampel- t-test.
Hasil penelitian menunjukkan bahwa dari hasil uji hipotesis di peroleh t hitung 7867 > t tabel 2,045. Hal ini menunjukkan H0 ditolak dan Ha diterima yang artinya terdapat pengaruh model two stay two stray melalui diagram fishbone terhadap keterampilan menulis kelas V SD Negeri 3 Puding Besar.
Desain Pembelajaran Penjumlahan dan Pengurangan Bilangan Bulat Menggunakan Matematika Realistik Konteks Permainanan Tradisional Congklak di SD Inpres Nunbaun Delha
Authors: Lantakay Chindy Novelin , Samo Damianus D. , Blegur Irna Karlina Sensiana 
Published: December 2024

Penelitian ini bertujuan untuk menghasilkan desain pembelajaran penjumlahan dan pengurangan bilangan bulat menggunakan matematika realistik konteks permainan tradisional congklak di SD Inpres Nunbaun Delha. Metode yang digunakan dalam penelitian ini yaitu desain research yang mana metode ini difokuskan pada pengembangan desain pembelajaran dalam bentuk hypothetical learning trajectory. Penelitian ini merupakan penelitian kualitatif yang melibatkan 6 orang siswa pada siklus pilot experiment dan 24 orang siswa pada siklus teching experiment. Teknik pengumpulan data yang digunakan adalah tes tertulis, catatan lapangan, wawancara dan dokumentasi. Sedangkan teknik analisis data dilakukan secara kualitatif. Penelitian ini menghasilkan suatu learning trajectory melalui pendekatan PMRI yang dapat digunakan untuk mendukung proses pembelajaran terkaitpenjumlahaan dan pengurangan bilangan bulat di SD Inpres Nunbaun Delha kelas VI. Penelitian ini menghasilkan suatu desain pembelajaran melalui pendekatan PMRI. Desain pembelajaran yang dihasilkan terdiri atas 7 aktivitas dimana pendekatan PMRI menjadi acuan utama dalam setiap aktivitas yang ada. Konteks yang digunakan pada penelitian ini adalah permainan tradisonal congklak. Siswa diminta bermain permainan tardisional congklak, Setelah itu, siswa mengamati proses bermain permainan tradisional congklak, Siswa diberikan congklak yang telah dimodifikasi cara bermainnya. Kemudian siswa menyelesaikan soal dengan bermain congklak yang telah dimodifikasi cara bermainnya. Selanjutnya siswa menggambar bulatan untuk menyelesaikan soal penjumlahaan dan pengurangan bilangan bulat. Setelah itu, siswa menyelesaikan soal tanpa menggunakan congklak maupun gambar bulatan dan diakhiri dengan siswa menyimpulkan konsep penjumlahaan dan pengurangan bilangan bulat.
PERANCANGAN SISTEM INFORMASI TARATIK BERBASIS WEBSITE DI SMK NEGERI 2 PARIAMAN
Authors: Gusmi Mardatil , Anggraini Samudra Ami , Kurniawan Haris 
Published: December 2024

Penelitian ini di latarbelakangi oleh poin siswa tidak terpantau secara real time dan pencatatan yang sering hilang. Sering kali laporan pelanggaran siswa hilang dan tidak dapat ditemukan karena pengarsipan masih dilakukan secara manual. Penelitian ini bertujuan untuk membangun system siswa dan merekap pelanggaran serta mendokumentasikan prestasi siswa dibidang akademik dan non-akedemik. Penelitian perancangan sistem informasi taratik berbasis website di SMK Negeri 2 Pariaman ini dilakukan dengan menggunakan metode Software Development life cycle (SDLC) dengan model iterative yaitu model pengembangan perangkat lunak yang menggunakan elemen yang diulang berkali-kali dan secara bersamaan. Hasil penelitian beta untuk validasi tenaga memperoleh hasil sangat setuju dengan rata -rata nilai 92,35% dan nilai rata – rata pernyataan survei pengguna adalah 97,55 % sangat baik. Dengan persentase tersebut, sistem informasi taratik berbasis website di SMK Negeri 2 Pariaman layak digunakan., Hasil pengujian beta untuk validasi tenaga memperoleh hasil sangat setuju dengan rata -rata nilai 92,35% dan nilai rata – rata pernyataan survei pengguna adalah 97,55 % sangat baik. Dengan persentase tersebut, sistem informasi taratik berbasis website di SMK Negeri 2 Pariaman layak digunakan.