bluuebunny/crossref_metadata_2025_split huggingface dataset:
Participation of actin filaments, myosin and phosphatidylinositol 3‐kinase in the formation and polarisation of tetraspore germ tube ofGelidium floridanum(Rhodophyta,Florideophyceae)
Authors: Filipin E. P. , Pereira D. T. , Ouriques L. C. , Bouzon Z. L. , Simioni C. 
Published: November 2018

AbstractThis study aimed to examine the evidence of direct interaction among actin, myosin and phosphatidylinositol 3‐kinase (PI3K) in the polarisation and formation of the tetraspore germ tube ofGelidium floridanum.After release, tetraspores were exposed to cytochalasin B, latrunculin B,LY294002 andBDMfor a period of 6 h.In control samples, formation of the germ tube occurred after the experimental period, with cellulose formation and elongated chloroplasts moving through the tube region in the presence of F‐actin. In the presence of cytochalasin B, an inhibitor of F‐actin, latrunculin B, an inhibitor of G‐actin, andBDM, a myosin inhibitor, tetraspores showed no formation of the germ tube or cellulose. Spherical‐shaped chloroplasts were observed in the central region with a few F‐actin filaments in the periphery of the cytoplasm. Tetraspores treated withLY294002, aPI3K inhibitor, showed no formation of the tube at the highest concentrations. Polarisation of cytoplasmic contents did not occur, only cellulose formation.It was concluded that F‐actin directs the cell wall components and contributes to the maintenance of chloroplast shape and elongation during germ tube formation.PI3K plays a fundamental role in signalling for the asymmetric polarisation of F‐actin. Thus, F‐actin regulates the polarisation and germination processes of tetraspores ofG. floridanum.
Post‐thawing effects of three cryopreservation diluents on Rusa deer (Rusa timorensis) spermatozoa
Authors: Monaco Davide , Zappia Eduardo , Apichaya Sudsukh , Lacalandra Giovanni M. , Thongtip Nikorn 
Published: November 2018

ContentsThe aim of this study was to evaluate home‐made and commercial extenders for the cryopreservation of Rusa deer semen. After collection by electroejaculation, six ejaculates were diluted and frozen in TES‐based, Tris‐based and Triladyl® extenders. Subjective motility, viability, morphology, acrosome integrity and membrane functionality were assessed post‐thawing and after 1‐hr incubation at 37°C (Thermal stress test). Total and progressive motility, and kinematic parameters were also assessed through CASA system. Post‐thawing sperm progressive motility (PM), velocity according to the straight path (VSL) and linearity (LIN) showed significant differences, and higher values were detected for spermatozoa diluted with Triladyl® and TES (p < 0.05) as compared with Tris (PM of Triladyl® 14.7% vs. 3.2% TES and 2.5% Tris; VSL 56 for Triladyl®, 59.2 for TES and 41.7 for Tris; LIN 45.6 for Triladyl®, 52 for TES and 36.5 for Tris). Triladyl® and TES extender led to better post‐thawing sperm parameters, but these preliminary results need to be verified through artificial insemination trials.
Effect of semen and donor factors on multiple ovulation and embryo transfer (MOET) in sheep
Authors: Bergstein‐Galan Tácia Gomes , Weiss Romildo Romualdo , Kozicki Luiz Ernandes 
Published: November 2018

ContentsMultiple ovulation and embryo transfer (MOET) is an important tool in the sheep industry for increasing numbers of genetically superior individuals. The objective of this study was to evaluate the effect of semen source (frozen or fresh), the number of embryo collection procedures for each donor (NECP), the season in which embryo transfer and collection was performed, and the age and breed of the donor, on the number of recovered embryos and pregnancy rates after embryo transfer. The Alamos Genetics’ flushing station database was used. This consisted of 140 embryo collection procedures, from 53 Dorper and White Dorper sheep donors, aged between one and eight years, totalling 1,200 collected embryos. Neither the number of retrieved embryos nor the pregnancy rate was affected by the semen preservation method (fresh or frozen), NECP or the age and breed of donor. The season did not affect the number of collected embryos but had a significant effect (p < 0.05) on the recipient pregnancy rate, with higher pregnancy rates reported in the winter (65.57% ± 25.33%) compared with spring (37.11% ± 33.27%), summer (29.95% ± 28.33%) or autumn (35.03% ± 31.66%). There is an estimated increase of 98.4% and 71.5% of embryos recovered in the spring and summer seasons, respectively, when winter is used as reference. The survival of embryos is significantly higher when implanted during the breeding season, more specifically in winter. Embryo collection can be carried out throughout the year in sheep, but there may be a marginal advantage in the use of superovulation and fresh embryo transfer programmes in the autumn and winter.
Phenotypic plasticity induced using high ambient temperature during embryogenesis in domesticated zebrafish, Danio rerio
Authors: Hosseini Shahrbanou , Brenig Bertram , Tetens Jens , Sharifi Ahmad Reza 
Published: November 2018

ContentsAmbient temperature during early stages of life has a substantial effect on physiological processes, eliciting phenotypic plasticity during zebrafish developmental stages. Zebrafish are known to possess a noteworthy ability to modify their phenotype in dependence of environmental factors. However, there is a poor understanding of the effects of temperature during embryogenesis, which influences the biological functions such as survival ability and masculinization in later developmental stages. Since the middle embryonic phase (pharyngula period) is genetically the most conserved stage in embryogenesis, it is very susceptible to embryonic lethality in developmental processes of vertebrates. Here, we tested the effect of transient perturbations (heat shock) during early development (5–24 hr post‐fertilization; hpf) at 35°C compared to control group at 28°C, on survival ability of zebrafish to study the embryonic and post‐embryonic mortality. We studied the variation of heat‐induced masculinization among and across the families in response to high temperature. Furthermore, morphometric traits of adult zebrafish at different developmental time points were measured in order to estimate the temperature × sex interaction effect. We found the highest embryonic mortality around the gastrula and segmentation periods in both experimental groups, with significantly lower survival ability in the temperature‐treated group (73.30% ± 0.58% vs. 70.19% ± 0.57%, respectively). A higher hatching success was observed in the control group (71.08% ± 0.61%) compared to the heat‐induced group (67.95% ± 0.60%). A distinct reduction in survival ability was also observed in both experimental groups during the first two weeks after hatching, followed by a reduced level of changes thereafter. We found sex ratio imbalances across all families, with 25.2% more males under temperature treatment. Our study on growth performance has shown a positive effect of increased temperature on growth plasticity, with a greater impact on female fish in response to high ambient temperature.
Eyetracking measures of social attention in young children: How gaze patterns translate to real‐life social behaviors
Authors: van Rijn Sophie , Urbanus Evelien , Swaab Hanna 
Published: November 2018

AbstractThe aim of this study was to evaluate to what degree eyetracking paradigms of social attention, in combination with synchronous measurements of affective arousal, were associated with real‐life social behavior of children aged 3–7 years. Several eyetracking paradigms were used, involving social interactions, single/multiple faces, and emotional faces. Arousal was measured using electrocardiography. Real‐life social behavior was measured using structured behavior observations, parent questionnaires, and developmental interviews. Time spent looking at social stimuli was significantly associated with real‐life social behaviors, and independent of age, IQ, or gender. Paradigms involving social interactions and looking time to the eyes showed the most consistent relations with social behaviors. Stronger affective arousal responses were associated with shorter looking times toward eyes, which in turn were associated with less social awareness in real life. Eyetracking and arousal measures allow for sensitive and objective assessment of social abilities that have great relevance for real‐life social behaviors, with the potential to use in a broad and diverse population. These measures may help gain insight into the underpinnings of social behavior and may serve as a valuable marker or outcome measure in understanding, monitoring, and stimulating social‐emotional development early in life.
Ureaplasma urealyticum pyelonephritis presenting with progressive dysuria, renal failure, and neurologic symptoms in an immunocompromised patient
Authors: Schwartz Drew J. , Elward Alexis , Storch Gregory A. , Rosen David A. 
Published: November 2018

AbstractUreaplasma urealyticum is a bacterial species correlated with urethritis in healthy individuals and invasive infections in immunocompromised patients. We describe a 20‐year‐old female with a history of remote heart transplant on everolimus, mycophenolate, and rituximab presenting with progressive urinary tract symptoms, renal failure, and neurologic symptoms. An extensive workup ultimately identified U urealyticum infection, and the patient successfully recovered after a course of azithromycin and doxycycline.
Metabolite profiling and genome‐wide association studies reveal response mechanisms of phosphorus deficiency in maize seedling
Authors: Luo Bowen , Ma Peng , Nie Zhi , Zhang Xiao , He Xuan , Ding Xin , Feng Xing , Lu Quanxiao , Ren Zhiyong , Lin Haijian , Wu Yuanqi , Shen Yaou , Zhang Suzhi , Wu Ling , Liu Dan , Pan Guangtang , Rong Tingzhao , Gao Shibin 
Published: November 2018

SUMMARYInorganic phosphorus (Pi) is an essential element in numerous metabolic reactions and signaling pathways, but the molecular details of these pathways remain largely unknown. In this study, metabolite profiles of maize (Zea mays L.) leaves and roots were compared between six low‐Pi‐sensitive lines and six low‐Pi‐tolerant lines under Pi‐sufficient and Pi‐deficient conditions to identify pathways and genes associated with the low‐Pi stress response. Results showed that under Pi deprivation the concentrations of nucleic acids, organic acids and sugars were increased, but that the concentrations of phosphorylated metabolites, certain amino acids, lipid metabolites and nitrogenous compounds were decreased. The levels of secondary metabolites involved in plant immune reactions, including benzoxazinoids and flavonoids, were significantly different in plants grown under Pi‐deficient conditions. Among them, the 11 most stable metabolites showed significant differences under low‐ and normal‐Pi conditions based on the coefficient of variation (CV). Isoleucine and alanine were the most stable metabolites for the identification of Pi‐sensitive and Pi‐resistant maize inbred lines. With the significant correlation between morphological traits and metabolites, five low‐Pi‐responding consensus genes associated with morphological traits and simultaneously involved in metabolic pathways were mined by combining metabolites profiles and genome‐wide association study (GWAS). The consensus genes induced by Pi deficiency in maize seedlings were also validated by reverse‐transcription quantitative polymerase chain reaction (RT‐qPCR). Moreover, these genes were further validated in a recombinant inbred line (RIL) population, in which the glucose‐6‐phosphate‐1‐epimerase encoding gene mediated yield and correlated traits to phosphorus availability. Together, our results provide a framework for understanding the metabolic processes underlying Pi‐deficient responses and give multiple insights into improving the efficiency of Pi use in maize.
LncRNA33732‐respiratory burst oxidase module associated with WRKY1 in tomato‐ Phytophthora infestans interactions
Authors: Cui Jun , Jiang Ning , Meng Jun , Yang Guanglei , Liu Weiwei , Zhou Xiaoxu , Ma Ning , Hou Xinxin , Luan Yushi 
Published: November 2018

SummaryOur previous studies indicated that tomato WRKY1 transcription factor acts as a positive regulator during tomato resistance to Phytophthora infestans. However, the molecular mechanism of WRKY1‐mediated resistance regulation remains unclear. Here, we used a comparative transcriptome analysis between wild‐type and WRKY1‐overexpressing tomato plants to identify differentially expressed genes (DEGs) and long non‐coding RNAs (DELs), and we examined long non‐coding RNA (lncRNA)‐gene networks. The promoter sequences of the upregulated DEGs and DELs were analyzed. Among 1073 DEGs and 199 DELs, 1 kb 5′‐upstream regions of 59 DEGs and 22 DELs contain the W‐box, the target sequence of the WRKY1. The results of promoter−β‐glucuronidase (GUS) fusion and yeast one‐hybrid assay showed that lncRNA33732 was activated by WRKY1 through sequence‐specific interactions with the W‐box element in its promoter. The overexpression and silencing analysis of lncRNA33732 in tomato showed that lncRNA33732 acts as a positive regulator and enhanced tomato resistance to P. infestans by induction of the expression of respiratory burst oxidase (RBOH) and increase in the accumulation of H2O2. When the expression of RBOH gene was inhibited in tomato plants, H2O2 accumulation decreased and resistance were impaired. These findings suggest that lncRNA33732 activated by WRKY1 induces RBOH expression to increase H2O2 accumulation in early defense reaction of tomato to P. infestans attack. Our results provide insights into the WRKY1−lncRNA33732−RBOH module involved in the regulation of H2O2 accumulation and resistance to P. infestans, as well as provide candidates to enhance broad‐spectrum resistance to pathogens in tomato.
Consequences of photosystem‐I damage and repair on photosynthesis and carbon use in Arabidopsis thaliana
Authors: Lima‐Melo Yugo , Gollan Peter J. , Tikkanen Mikko , Silveira Joaquim A. G. , Aro Eva‐Mari 
Published: November 2018

SummaryNatural growth environments commonly include fluctuating conditions that can disrupt the photosynthetic energy balance and induce photoinhibition through inactivation of the photosynthetic apparatus. Photosystem II (PSII) photoinhibition is efficiently reversed by the PSII repair cycle, whereas photoinhibited photosystem I (PSI) recovers much more slowly. In the current study, treatment of the Arabidopsis thaliana mutant proton gradient regulation 5 (pgr5) with excess light was used to compromise PSI functionality in order to investigate the impact of photoinhibition and subsequent recovery on photosynthesis and carbon metabolism. The negative impact of PSI photoinhibition on CO2 fixation was especially deleterious under low irradiance. Impaired starch accumulation after PSI photoinhibition was reflected in reduced respiration in the dark, but this was not attributed to impaired sugar synthesis. Normal chloroplast and mitochondrial metabolisms were shown to recover despite the persistence of substantial PSI photoinhibition for several days. The results of this study indicate that the recovery of PSI function involves the reorganization of the light‐harvesting antennae, and suggest a pool of surplus PSI that can be recruited to support photosynthesis under demanding conditions.
Comparing approval procedures for new drugs
Authors: Houy Nicolas , Jelovac Izabela 
Published: November 2018

AbstractWe analyse how drug approval procedures influence the incentives of pharmaceutical firms to commercialise new drugs in the presence of international reference pricing. Since 1995, the European Medicines Agency (EMA) coordinates a centralised approval procedure for specific new drugs in the EU. With such a centralised procedure, the EMA grants simultaneous drug approvals for all EU countries. Alongside, non‐centralised procedures coexist for approvals of other new drugs and approvals can be sequential. We focus on the effects of the exogenous timing of drug approvals, either simultaneous or sequential, and we compare the effects of centralised versus non‐centralised procedures on the firms’ incentives to commercialise new drugs in different markets. In a context of international reference pricing, we show that a centralised procedure limits the number of countries where the firm commercialises new drugs, compared to a non‐centralised procedure. We also show in a simplified framework that countries are better off with non‐centralised procedures, while pharmaceutical firms in theory prefer the drug to be approved everywhere as soon as possible, as in an early centralised procedure. This is in line with the stated objective of the EMA to generate savings for the pharmaceutical firms.
Phenotypic similarity leads to taxonomic inconsistency: A revision of the lowland's antpittas
Authors: Carneiro Lincoln , Bravo Gustavo A. , Aleixo Alexandre 
Published: November 2018

AbstractA comprehensive molecular phylogeny of lowland antpittas in the genera Hylopezus and Myrmothera indicated that Hylopezus, as currently defined, is paraphyletic with respect to Myrmothera and Grallaricula. Specifically, both species now placed in Myrmothera, Hylopezus dives, Hylopezus fulviventris and Hylopezus berlepschi form a strongly supported clade that is sister to a clade comprised by Hylopezus perspicillatus, Hylopezus auricularis, Hylopezus ochroleucus, Hylopezus whittakeri, Hylopezus paraensis, Hylopezus macularius, and Hylopezus dilutus. Furthermore, Hylopezus nattereri is sister to a clade glade grouping Myrmothera, Hylopezus, and Grallaricula, representing the most divergent lineage in this complex. Our approach to assess diagnosability and define generic boundaries among these taxa integrates phylogenetic relationships with morphological and acoustic traits. Given that phenotypic and ecological differences do not warrant merging H. nattereri into any other genus, and because there is no generic name available for H. nattereri, we describe herein a new genus for this Atlantic Forest endemic lineage, Cryptopezus gen. n. We also redefine generic limits in Myrmothera and Hylopezus to have a taxonomic classification concordant with their phylogenetic relationships.
Understanding the full burden of drowning: a retrospective, cross-sectional analysis of fatal and non-fatal drowning in Australia
Authors: Peden Amy E , Mahony Alison J , Barnsley Paul D , Scarr Justin 
Published: November 2018

ObjectivesThe epidemiology of fatal drowning is increasingly understood. By contrast, there is relatively little population-level research on non-fatal drowning. This study compares data on fatal and non-fatal drowning in Australia, identifying differences in outcomes to guide identification of the best practice in minimising the lethality of exposure to drowning.DesignA subset of data on fatal unintentional drowning from the Royal Life Saving National Fatal Drowning Database was compared on a like-for-like basis to data on hospital separations sourced from the Australian Institute of Health and Welfare’s National Hospital Morbidity Database for the 13-year period 1 July 2002 to 30 June 2015. A restrictive definition was applied to the fatal drowning data to estimate the effect of the more narrow inclusion criteria for the non-fatal data (International Classification of Diseases (ICD) codes W65-74 and first reported cause only). Incidence and ratios of fatal to non-fatal drowning with univariate and Χ2analysis are reported and used to calculate case-fatality rates.SettingAustralia, 1 July 2002 to 30 June 2015.ParticipantsUnintentional fatal drowning cases and cases of non-fatal drowning resulting in hospital separation.Results2272 fatalities and 6158 hospital separations occurred during the study period, a ratio of 1:2.71. Children 0–4 years (1:7.63) and swimming pools (1:4.35) recorded high fatal to non-fatal ratios, whereas drownings among people aged 65–74 years (1:0.92), 75+ years (1:0.87) and incidents in natural waterways (1:0.94) were more likely to be fatal.ConclusionsThis study highlights the extent of the drowning burden when non-fatal incidents are considered, although coding limitations remain. Documenting the full burden of drowning is vital to ensuring that the issue is fully understood and its prevention adequately resourced. Further research examining the severity of non-fatal drowning cases requiring hospitalisation and tracking outcomes of those discharged will provide a more complete picture.
Synthesis and Polymerization of Naphthoxazines Containing Furan Groups: An Approach to Novel Biobased and Flame-Resistant Thermosets
Authors: de Oliveira Davi Rabelo , Mazzetto Selma Elaine , Lomonaco Diego 
Published: November 2018

Naphthoxazines are a class of compounds with potential application in obtaining high-performance polymeric materials. Such application of these compounds, however, is still scarcely explored in the literature. Combined with the search for new high-performance materials, the development of biobased polymers has gained a lot of attention. In this sense, the inclusion of furan groups in polymers has been explored as a strategy that combines the search for high-performance materials with the search for the development of biobased materials. In this work, novel naphthoxazine monomers containing furan groups were synthetized. The syntheses were carried out in a single step, without the use of solvents and catalysts, obtaining the products in satisfactory yields and high purity. The naphthoxazines had their chemical structures completely characterized by FTIR, 1H NMR, and 13C NMR techniques. The thermal analyses (DSC and TGA) showed that all naphthoxazines exhibit exothermic typical polymerization events, making these compounds suitable for obtention of poly(naphthoxazines) resins, but suffer significant mass losses at temperatures below the onset polymerization temperature. In this way, a catalyst (1 mol% MgCl2) was used in order to allow the polymerization of the compounds before the mass loss events. The FTIR analysis showed strong evidences of the formation of poly(naphthoxazines), and TGA analyses showed that the resins have high thermal stability, with high flame resistance and self-extinguishing properties (LOI > 28), which makes these compounds attractive in the development of biobased and high-performance materials.
Innovation in newly public firms: The influence of government grants, venture capital, and private equity
Authors: Shinkle George A , Suchard Jo-Ann 
Published: November 2018

We investigate the influence of government grants, venture capital (VC), and private equity (PE) funding on innovation in newly public firms. We examine innovation inputs (R&D), innovation outputs (patents), and the quality thereof (patent citations). We contribute to understanding of the mechanisms between government grants and subsequent VC and PE funding and innovation. We find that grants encourage VC funding but not PE funding. Grants and VC/PE funding are generally complements regarding innovation except grants substitute for VC funding on innovation inputs. Furthermore, we observe that the firm-level heterogeneity of VC/PEs significantly influences innovation in portfolio companies. JEL Classification: G24, G32, L53, O31, O38