bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global outcomes and prognosis for relapsed/refractory mature T-cell and NK-cell lymphomas: results from the PETAL consortium
Authors: Han Jessy Xinyi , Koh Min Jung , Boussi Leora , Sorial Mark , McCabe Sean M. , Peng Luke , Singh Shambhavi , Eche-Ugwu Ijeoma Julie , Gabler Judith , Fernandez Turizo Maria J. , MacVicar Caroline T. , Garg Aditya , Disciullo Alexander , Chopra Kusha , Lenart Alexandra , Nwodo Emmanuel , Barnes Jeffrey , Koh Min Ji , Miranda Eliana , Chiattone Carlos , Stuver Robert , Horwitz Steven M. , Merrill Mwanasha , Jacobsen Eric , Manni Martina , Civallero Monica , Skrypets Tetiana , Lymboussaki Athina , Federico Massimo , Kim Yuri , Kim Jin Seok , Cho Jae Yong , Eipe Thomas , Shet Tanuja , Sridhar Epari , Shetty Alok , Saha Saswata , Jain Hasmukh , Sengar Manju , Van Der Weyden Carrie , Prince Henry Miles , Hamouche Ramzi , Murdashvili Tinatin , Foss Francine , Gentilini Marianna , Casadei Beatrice , Zinzani Pier Luigi , Okatani Takeshi , Yoshida Noriaki , Yoon Sang Eun , Kim Won-Seog , Panchoo Girisha , Mohamed Zainab , Verburgh Estelle , Alturas Jackielyn Cuenca , Al-Mansour Mubarak , Ford Josie , Cabrera Maria Elena , Ku Amy , Bhagat Govind , Ma Helen , Sawas Ahmed , Kariya Khyati Maulik , Iwasaki Makoto , Bhanushali Forum , O’Connor Owen A. , Marchi Enrica , Shen Changyu , Shah Devavrat , Jain Salvia 
Published: October 2024

Abstract
Variances in global access to drugs and treatment practices make it challenging to understand the benefit of contemporary therapies in patients with relapsed and refractory (R/R) mature T-cell and natural killer–cell lymphomas (MTCL and MNKCL). We conducted an international retrospective cohort study of 925 patients with R/R MTCL and MNKCL. In peripheral T-cell lymphoma–not otherwise specified and anaplastic lymphoma kinase–negative anaplastic large cell lymphoma (ALK– ALCL), patients with relapsed lymphoma demonstrated a superior median overall survival (OS) relative to refractory from the time of second-line treatment. We identified several independent predictors of OS for R/R lymphoma including age &gt;60 years, primary refractory disease, histological subtype other than angioimmunoblastic T-cell lymphoma (AITL), extranodal sites &gt;1, Ki67 ≥40%, and absolute lymphocyte count less than the lower limit of normal. A multivariable model incorporating these formed the basis for a prognostic index for R/R TCL, in which patients are stratified into low-risk (0-1 risk factor), intermediate-risk (2-3 risk factors), or high-risk (≥4 risk factors) groups, which were associated with 3-year OS of 57.14%, 23.3%, and 7%, respectively. Patients received either a "novel" single agent (SA; 35%) or cytotoxic chemotherapy (CC; 60%) for their second-line treatment. Higher progression-free survival was observed with SA over CC for the entire cohort with a higher 3-year OS in AITL and ALK– ALCL. Among the SA, small-molecule inhibitors demonstrated OS advantage relative to CC in AITL. Our results highlight continued efficacy of novel drugs globally and the potential of a new prediction model in informing heterogeneous prognosis within the R/R population of MTCL and MNKCL.
Experiences of lifestyle changes among Thai older adults six months after applying the Plan-Do-Study-Act (PDSA) cycle
Authors: Wongsala Manothai , Rosendahl Sirpa , Anbäcken Els-Marie , Manasatchakun Pornpun , Holmgren Jessica 
Published: October 2024

Abstract
Background
Thai older adults are valuable resources in their society. The Thai health service system is challenged when it comes to ensuring that older Thai adults can continue to live healthy and independent lives in society. It is of great value to support independence and improve older people’s active ageing. Promoting lifestyle changes by applying the Plan-Do-Study-Act cycle (PDSA cycle), at group meetings in a municipality context, is a way of focusing on active ageing. This study aims to describe older adults´ experiences of lifestyle change six months after finishing group meetings applying the PDSA cycle.

Methods
A qualitative approach with individual interviews and a qualitative content analysis were used with 12 Thai older adults who participated in the meetings applying the PDSA cycle.

Results
Six months after finishing applying the PDSA cycle, some older adults kept their individual goals and were influenced by their family surroundings. They also formulated additional goals. Three categories and six sub-categories emerged: Keeping individual goals, influenced by the surroundings, and formulation of additional goals were the overall categories.

Conclusions
These Thai older adults showed that they had the ability to make lifestyle changes with the support of the PDSA cycle, but not all maintained their planned activities after six months. The question is how healthcare professionals and the surroundings, may further support and motivate these people to maintain these changes based on their own preferences in a sustainable way.
Sex differences in contextual fear conditioning and extinction after acute and chronic nicotine treatment
Authors: Keady Jack V. , Hessing Marissa C. , Songrady Judy C. , McLaurin Kristen , Turner Jill R. 
Published: October 2024

Abstract
Background
Chronic cigarette smokers report withdrawal symptomology, including affective dysfunction and cognitive deficits. While there are studies demonstrating sex specific withdrawal symptomology in nicotine-dependent individuals, literature examining the underlying biological mediators of this is scant and not in complete agreement. Therefore, in this study, we evaluated the sex specific effects of nicotine and withdrawal on contextual fear memory, a hippocampally dependent aspect of cognition that is disrupted in nicotine withdrawal.

Methods
Male and female B6/129F1 mice (8–13 weeks old) were used in all experiments. For the acute nicotine experiment, mice received intraperitoneal saline or nicotine (0.5 mg/kg) prior to contextual fear conditioning and test. For the chronic nicotine experiment, mice received nicotine (18 mg/kg/day) or saline for 11 days, then underwent contextual fear conditioning and test. Following the test, mice underwent minipump removal to elicit withdrawal or sham surgery, followed by the fear extinction assay. Bulk cortical tissue was used to determine nicotinic acetylcholine receptor levels via single point [3H]Epibatidine binding assay. Gene expression levels in the dorsal and ventral hippocampus were quantified via RT-PCR.

Results
We found that female mice had a stronger expression of contextual fear memory than their male counterparts. Further, following acute nicotine treatment, male, but not female, subjects demonstrated augmented contextual fear memory expression. In contrast, no significant effects of chronic nicotine treatment on fear conditioning were observed in either sex. When examining extinction of fear learning, we observed that female mice withdrawn from nicotine displayed impaired extinction learning, but no effect was observed in males. Nicotine withdrawal caused similar suppression of fosb, cfos, and bdnf, our proxy for neuronal activation and plasticity changes, in the dorsal and ventral hippocampus of both sexes. Additionally, we found that ventral hippocampus erbb4 expression, a gene implicated in smoking cessation outcomes, was elevated in both sexes following nicotine withdrawal.

Conclusions
Despite the similar impacts of nicotine withdrawal on gene expression levels, fosb, cfos, bdnf and erbb4 levels in the ventral hippocampus were predictive of delays in female extinction learning alone. This suggests sex specific dysfunction in hippocampal circuitry may contribute to female specific nicotine withdrawal induced deficits in extinction learning.
Sex dimorphism and tissue specificity of gene expression changes in aging mice
Authors: Zhu Dantong , Arnold Matt , Samuelson Brady A. , Wu Judy Z. , Mueller Amber , Sinclair David A. , Kane Alice E. 
Published: October 2024

Abstract
Background
Aging is a complex process that involves all tissues in an organism and shows sex dimorphism. While transcriptional changes in aging have been well characterized, the majority of studies have focused on a single sex and sex differences in gene expression in aging are poorly understood. In this study, we explore sex dimorphism in gene expression in aging mice across three tissues.

Methods
We collected gastrocnemius muscle, liver and white adipose tissue from young (6 months, n = 14) and old (24 months, n = 14) female and male C57BL/6NIA mice and performed RNA-seq. To investigate sex dimorphism in aging, we considered two levels of comparisons: (a) differentially expressed genes between females and males in the old age group and (b) comparisons between females and males across the aging process. We utilized differential expression analysis and gene feature selection to investigate candidate genes. Gene set enrichment analysis was performed to identify candidate molecular pathways. Furthermore, we performed a co-expression network analysis and chose the gene module(s) associated with aging independent of sex or tissue-type.

Results
We identified both tissue-specific and tissue-independent genes associated with sex dimorphism in aged mice. Unique differentially expressed genes between old males and females across tissues were mainly enriched for pathways related to specific tissue function. We found similar results when exploring sex differences in the aging process, with the exception that in the liver genes enriched for lipid metabolism and digestive system were identified in both females and males. Combining enriched pathways across analyses, we identified amino acid metabolism, digestive system, and lipid metabolism as the core mechanisms of sex dimorphism in aging. Although the vast majority of age-related genes were sex and tissue specific, we identified 127 hub genes contributing to aging independent of sex and tissue that were enriched for the immune system and signal transduction.

Conclusions
There are clear sex differences in gene expression in aging across liver, muscle and white adipose. Core pathways, including amino acid metabolism, digestive system and lipid metabolism, contribute to sex differences in aging.
Comparison of facilities with and without additional medical fees for nutrition support team activity during the COVID-19 pandemic
Authors: Futamura Akihiko , Koseki Takenao , Iida Junichi , Suzuki Akito , Muroi Nobuyuki , Myotoku Michiaki , Maki Hiroki , Mizutani Kazuhisa , Ogino Hikaru , Taniguchi Yasuki , Higashi Keiichiro , Usui Masanobu 
Published: October 2024

Abstract
Background
This study aimed to clarify the effectiveness of nutrition support team (NST) facilities for preventing central line-associated bloodstream infection (CLABSI).

Methods
We retrospectively analyzed the incidence of CLABSI as well as the presence or absence of additional medical fees for NST activity between 2019 and 2021, including the period before and after the COVID-19 pandemic. Subsequently, we performed between-group comparisons of the CLABSI incidence. CLABSI rates were compared based on cumulative per 1000 catheter uses during the relevant period.

Results
Among 47 facilities that were registered for participation, there were 34 and 13 facilities with and without additional medical fees for NST activity (NST and non-NST groups, respectively). The CLABSI incidence rate was significantly lower in the NST group 0.96 [0.28–1.73] than in the non-NST group 1.25 [075–6.10] (p < 0.05). Before the pandemic, the NST group had a lower CLABSI rate per 1000 catheter uses than the non-NST group 2019: 0.70 [0.12–1.26] vs 2.10 [0.62–5.97]. During the pandemic, the CLABSI incidence showed no significant between-group difference 2020: 0.99 [0.51–1.61] vs 1.01 [0.80–4.16]; 2021: 1.24 [0.44–2.35] vs 1.96 [1.23–5.31]; however, the CLABSI rates in the NST group remained low.

Conclusion
During the COVID-19 pandemic, the incidence of CLABSI was lower in the NST group than in the non-NST group, indicating the effectiveness of NST in preventing the occurrence of CLABSI.
Prescribed fires effects on actual and modeled fuel loads and forest structure in southern coast redwood (Sequoia sempervirens) forests
Authors: Katuna Taj A. , Collins Brandon M. , Stephens Scott L. 
Published: October 2024

Abstract
Background
Fire suppression, timber harvesting, and the forced removal of Indigenous burning have fundamentally changed conditions in coast redwood forests. The contemporary approach of forest preservation and fire exclusion has produced high densities of small trees, elevated fuel loads, and increased vulnerability to wildfire and climate change. Prescribed broadcast burning presents a viable treatment option to meet forest management goals, especially where mechanical treatments are not feasible. Forest and fire managers utilizing fire modeling software such as the Fire and Fuels Extension of Forest Vegetation Simulator (FFE) to predict prescribed fire effects in redwoods are limited by model accuracy due to a lack of empirical research and model verification across a breadth of site conditions.

Results
We compared the difference between pre- and post-treatment conditions for two fall-season prescribed burns in Sonoma and Santa Cruz counties in California to quantify changes to forest structure, fuel loads, and modeled wildfire hazard. Observed data was used to analyze the accuracy of FFE modeled prescribed fire treatment outputs for post-treatment forest and fuel conditions. Observed burn treatments were low intensity and resulted in no significant change to forest structure and composition, but there was a reduction in seedling and sapling densities and an increase in resprout density. There was a reduction in duff and litter fuels, and litter and fine woody debris reduction was driven by pre-treatment total fuel loads. The modeled probability of torching was very low pre- and post-treatment. FFE underpredicted scorch height, duff fuel reduction, and redwood regeneration, but slightly overpredicted tree mortality and significantly overpredicted reduction of litter and fine woody debris.

Conclusion
Our results highlight a need for model refinement in regard to species-specific mortality, tree regeneration dynamics, fuel recruitment and deposition, and moisture-dependent fuel consumption. In order to achieve desired forest management goals, fire practitioners may need to burn at moderate to high intensities, and potentially pair burning with mechanical thinning. Long-term health of coast redwood forests also relies on the restoration of cultural fire and stewardship partnerships that equally share decision making power between western science and Indigenous knowledge bearers.
Circulating microRNA-21, microRNA-122, and microRNA-222 as diagnostic biomarkers for hepatitis c virus-related hepatocellular carcinoma
Authors: Hetta Helal F. , Hamed Hager M. , Mekky Mohamed A. , Abdel-Malek Mohamed O. , Hassan Waleed Attia 
Published: October 2024

Abstract
Background and aim
MicroRNAs (miRs) are now a well-known subject in various tumor genesis and are studied as early diagnostic biomarker. Many arrays of miRs were incorporated in the pathogenesis of HCV-related hepatocellular carcinomas (HCV-HCC). In this respect, we aimed to evaluate the diagnostic role of circulating miR-21, miR-122, and miR-222 in Egyptian patients with HCV-HCC.

Patient and methods
Between June 2018 and April 2019, a cross-sectional comparative study was designed to evaluate the circulating miR-21, miR-122, and miR-222 by quantitative Real-Time PCR. For analytical purposes, patients were categorized into three groups: chronic HCV group (CHC-group, n = 22), HCV-related liver cirrhosis (LC-group, n = 22), and HCV-related hepatocellular carcinoma (HCV-HCC-group, n = 54).

Results
Serum levels of miR-21 and miR-222 increased with the progressive course from CHC to LC and HCC; p < .001. Serum levels of miR-122 in HCC patients were significantly lower than non-HCC patients (CHC and LC patients, n = 44); p < .001. However, the differences in levels of serum miR-122 between CHC and LC were not statistically significant; P = 0.8.
ROC curve analysis showed that the sensitivity and specificity of miR-21 were 61.1% and 95.5%, miR-222 were 71.7% and 93.2%, and miR-122 were 98.2% and 100%. The positive predictive value for miRNA-21, miRNA-122, and miRNA-222 were 13.4%, 93.3%, and 10.5% respectively. The Negative predictive value for miRNA-21, miRNA-122, and miRNA-222 were 94.3%, 97.8%, and 92.7% respectively.

Conclusion
MiR-21 and miR-222 could be potential markers for advanced liver damage, while miR-122 had the best diagnostic accuracy and could be a promising marker for detection of HCC.
Weak field deflection angle and analytical parameter estimation of the Lorentz-violating Bumblebee parameter through the black hole shadow using EHT data
Authors: Lambiase Gaetano , Pantig Reggie C. , Övgün Ali 
Published: October 2024

Abstract
We explored how the Lorentz symmetry breaking parameter ℓ affects the Reissner-Nordstöm BH solution in the context of weak field deflection angle, and the black hole shadow. We aim to derive the general expression for the weak deflection angle using the non-asymptotic version of the Gauss-Bonnet theorem, and we presented a way to simplify the calculations under the assumption that the distance of the source and the receiver are the same. Through the Solar System test, ℓ is constrained from around

orders of magnitude to 0, implying challenging detection of ℓ through the deflection of light rays from the Sun. We also studied the black hole shadow in an analytic way, where we applied the EHT results under the far approximation in obtaining an estimate expression for ℓ. Using the realistic values of the black hole mass and observer distance for Sgr. A* and M87*, it was shown that

is satisfied, implying the relevance and potential promise of the spontaneous Lorentz symmetry breaking parameter's role on the shadow radius uncertainties as measured by the EHT. We find constraints for ℓ to be negatively valued, where the upper and lower bounds are ∼ - 1.94 and ∼ - 2.04, respectively.
Reliable replicative lifespan determination of yeast with a single-channel microfluidic chip
Authors: Salzman Valentina , Torres Moises R. Bustamante , Tedesco Francisco G. Correa , Tarkowski Nahuel , Willems María J. Godás , Bravo Joaquín N. , Mercuri Magalí , Mercado Dante G. , Berlin Guido , Bellino Martín G. , Aguilar Pablo S. , Estrada Laura C. 
Published: October 2024

ABSTRACT
Saccharomyces cerevisiae is a powerful model for aging research due to its short lifespan and genetic malleability. Microfluidic devices offer an attractive approach enabling rapid monitoring of hundreds of cells during their entire replicative lifespan (RLS). Yet, key operational issues such as contaminations, cell loss, and cell-aggregates-dependent flow obstruction can hinder RLS experiments. We report the development of a microfluidic device configuration that effectively prevents flow blockage. We conducted comprehensive performance characterization, evaluating trapping efficiency, cell retention, budding orientation, and cell aggregate formation. The optimized device successfully supported long-term culturing and reliable RLS measurements of budding yeast strains. For accurate lifespan determination, a detailed workflow is provided that includes device fabrication, live microscopy setup, and characterization of cell age distribution. This work describes an accessible and reliable microfluidic device for yeast RLS studies, promoting further exploration in aging research.
Cell–cell junctions in focus – imaging junctional architectures and dynamics at high resolution
Authors: Janssen Vera , Huveneers Stephan 
Published: October 2024

ABSTRACT
Studies utilizing electron microscopy and live fluorescence microscopy have significantly enhanced our understanding of the molecular mechanisms that regulate junctional dynamics during homeostasis, development and disease. To fully grasp the enormous complexity of cell–cell adhesions, it is crucial to study the nanoscale architectures of tight junctions, adherens junctions and desmosomes. It is important to integrate these junctional architectures with the membrane morphology and cellular topography in which the junctions are embedded. In this Review, we explore new insights from studies using super-resolution and volume electron microscopy into the nanoscale organization of these junctional complexes as well as the roles of the junction-associated cytoskeleton, neighboring organelles and the plasma membrane. Furthermore, we provide an overview of junction- and cytoskeletal-related biosensors and optogenetic probes that have contributed to these advances and discuss how these microscopy tools enhance our understanding of junctional dynamics across cellular environments.
The Questionnaire for Experiencing and Using Violence in intimate relationships
Authors: Dyjakon Dorota Jolanta , Rajba Beata Marta 
Published: October 2024

Cel pracyCelem skonstruowania i walidacji Kwestionariusza Poczucia Doznawania i Stosowania Przemocy w relacji intymnej (KPDiSP) jest pomoc w diagnozie i wzbogacenie kompetencji psychoterapeutów pracujących z osobami doświadczającymi trudności w związkach intymnych.MetodaKwestionariusz składa się z ośmiu twierdzeń dotyczących poczucia doznawania i stosowania przemocy. Ustosunkowując się do kolejnych twierdzeń w pięciostopniowej skali, osoba może określić poziom doznawania, ale także i stosowania przemocy. KPDiSP pozwala mierzyć poziom doświadczania i stosowania przemocy w czterech podstawowych wymiarach – emocjonalnym, seksualnym, ekonomicznym i fizycznym.WynikiNa podstawie całości przeprowadzonych analiz można stwierdzić, że kwestionariusz cechuje dobra rzetelność. KPDiSP może być używany przez terapeutów zarówno jako narzędzie służące ocenie natężenia doznawania i stosowania przemocy, ale także przez pacjentów jako materiał do analizy własnych doznań oraz zachowań partnera, pomagając im zdobyć świadomość trudności, a czasem i zagrożeń w relacji intymnej.WnioskiZaletą tego kwestionariusza jest także krótka forma ułatwiającą terapeutom i pacjentom szybkie zapoznanie się z treścią.
High-performance 320-nm continuous-wave solid-state laser
Authors: Xu Guofeng , Wang Shuxian , Liang Fei , Lu Dazhi , Wu Kui , Zhang Huaijin , Yu Haohai , Yu Pingzhang , Wang Zhengping 
Published: October 2024

For the first time, to our knowledge, we realized a high-performance 320-nm continuous-wave (CW) laser with a fiber-coupled blue laser diode as the pump source. A V-shaped folded cavity is constituted by three mirrors, with a Pr3+:LiYF4 (Pr:YLF) as the laser crystal and a LiB3O5 (LBO) as the frequency doubling crystal, respectively. Under an absorbed pump power of 19.5 W, the maximum 320-nm output power reaches 4.26 W, corresponding to an optical conversion efficiency of 21.8% and a root-mean-square (RMS) power fluctuation of 0.61%, which give the best results of a 320-nm CW laser up to now. The excellent performance will make this ultraviolet laser source very suitable for practical applications, including semiconductor detection, spectral analysis, microscopic imaging, and biotechnology.