bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermal Cycling Reliability on Encapsulated Flexible Printed Circuit Fabricated With Water-Based Ink and Room-Temperature Curable Adhesive
Authors: Lall Pradeep , Bimali Sabina , Miller Scott 
Published: November 2024

Abstract
The application of room-temperature curable adhesive and water-based ink offers an economical and sustainable method in the field of electronics production. On the other hand, little is known about how encapsulation and thermal cycling affect the functionality of circuits fabricated using these materials. The purpose of this study is to look into how thermal cycling circumstances and encapsulation techniques affect the reliability and performance of circuits made with room-temperature curable adhesive and water-based ink. Understandings from this research will fill this knowledge gap and improve the processes involved in the design and production of environmentally friendly electronic circuits, leading to the development of sustainable electronics technology. Even with the progress made in electronics production, not much is known about how encapsulation will affect the thermal cycling reliability of a circuit made with water-based ink and room-temperature curable adhesive. Comprehensive studies addressing the performance and reliability test of circuits made using this environmentally friendly method are lacking in the current literature. This lack of systematic studies that examine these circuits’ behavior in different environmental settings — like thermal cycling with encapsulation procedures — is the limitation. As a result, the lack of knowledge makes it difficult to optimize manufacturing procedures and compromises the production of robust, reliable electronics made of eco-friendly materials. It is crucial to address this shortcoming to further sustainable electronics technology and encourage the electronics industry to embrace environmentally friendly manufacturing practices. This paper investigates the effect of encapsulation in flexible printed circuits fabricated with water-based inks and room-temperature curable adhesives, undergoing thermal cycling processes. A comparison will be made between the encapsulated and non-encapsulated circuits manufactured with room-temperature processable interconnects, coupled with water-based inks, for both test designs: individual components test and functional circuit test. The study includes the realization of a differentiator circuit through aerosol jet printing, employing room temperature interconnects for component attachment, and comparing the performance of encapsulated circuits with that of the non-encapsulated circuit, under Thermal Cycling.
State of Health Estimation Model Development for Li-Ion Battery Lifetime Considering Calendar Aging and Post-Knee Degradation
Authors: Lall Pradeep , Soni Ved , Bimali Sabina 
Published: November 2024

Abstract
Lithium-ion batteries are the most popular choice for power sources in the modern consumer electronics products, due to their exceptional specific energy, specific capacity, and other features including long cycle life, high columbic efficiency, and low self-discharge rate. To address the modern and ubiquitous concerns of improving product sustainability and counter supply-chain shortages, understanding battery degradation is pivotal for extending battery life, improving battery materials, and informing end user about device battery health to prompt replacement as needed. Battery health degradation as a phenomenon starts even before the battery is put into use in a device, as the battery capacity degrades during storage (calendar aging); and continues even after its cycling life is completed, wherein the battery experiences catastrophic failure as its health degrades rapidly (knee-point failure). The majority of the models available in literature today focus mainly of estimating battery health during its usage phase. However, it is critical to model battery health in the pre-use phase, to understand the battery health status when its put into use, and even after the battery has experienced knee-point failure to inform the user of the need for urgent replacement. This study proposes a cell lifetime degradation model which addresses all the three aforementioned phases of battery life: calendar aging, cycle aging, and post-knee aging. By testing batteries using different case-specific parameters, battery degradation data has been acquired for different aging stages. This data is then used to build individual models for the three stages which have later been combined to form the cell lifetime degradation model. Moreover, the study provides markers to estimate onset of knee-point failure using battery parameters that can be made accessible to the end user.
Sequential High Humidity and Isothermal Evolution of UF-Substrate Interface
Authors: Lall Pradeep , Choudhury Padmanava , Pandurangan Aathi 
Published: November 2024

Abstract
FCBGA architecture has become increasingly complex, with increases their power requirements, and in conjunction, these packages are ubiquitous modern-day automotive platforms. The assurance of the reliability of the packages requires a fundamental understanding of the damage accrual at the interfaces. Underfills are used to provide supplemental support to the interconnects between the chip and substrate. Long-term high humidity and high temperature are detrimental environmental stresses which may increase the propensity for the premature failure of the interface followed by interconnect failures. In this study, the objective is to analyze the interface strength reliability between underfill/substrate due to sequential high-temperature and high humidity operation.
Frequent genetic exchanges revealed by a pan-mitogenome graph of a fungal plant pathogen
Authors: van Westerhoven Anouk C. , Dijkstra Jelmer , Aznar Palop Jose L. , Wissink Kyran , Bell Jasper , Kema Gert H. J. , Seidl Michael F. 
Published: November 2024

ABSTRACT

Mitochondria are present in almost all eukaryotic lineages. The mitochondrial genomes (mitogenomes) evolve separately from nuclear genomes, and they can therefore provide relevant insights into the evolution of their host species.
Fusarium oxysporum
is a major fungal plant pathogen that is assumed to reproduce clonally. However, horizontal chromosome transfer between strains can occur through heterokaryon formation, and recently, signs of sexual recombination have been observed. Similarly, signs of recombination in
F. oxysporum
mitogenomes challenged the prevailing assumption of clonal reproduction in this species. Here, we construct, to our knowledge, the first fungal pan-mitogenome graph of nearly 500
F
.
oxysporum
mitogenome assemblies to uncover the variation and evolution. In general, the gene order of fungal mitogenomes is not well conserved, yet the mitogenome of
F. oxysporum
and related species are highly colinear. We observed two strikingly contrasting regions in the
F. oxysporum
pan-mitogenome, comprising a highly conserved core mitogenome and a long variable region (6–16 kb in size), of which we identified three distinct types. The pan-mitogenome graph reveals that only five intron insertions occurred in the core mitogenome and that the long variable regions drive the difference between mitogenomes. Moreover, we observed that their evolution is neither concurrent with the core mitogenome nor with the nuclear genome. Our large-scale analysis of long variable regions uncovers frequent recombination between mitogenomes, even between strains that belong to different taxonomic clades. This challenges the common assumption of incompatibility between genetically diverse
F. oxysporum
strains and provides new insights into the evolution of this fungal species.

IMPORTANCE

Insights into plant pathogen evolution is essential for the understanding and management of disease.
Fusarium oxysporum
is a major fungal pathogen that can infect many economically important crops. Pathogenicity can be transferred between strains by the horizontal transfer of pathogenicity chromosomes. The fungus has been thought to evolve clonally, yet recent evidence suggests active sexual recombination between related isolates, which could at least partially explain the horizontal transfer of pathogenicity chromosomes. By constructing a pan-genome graph of nearly 500 mitochondrial genomes, we describe the genetic variation of mitochondria in unprecedented detail and demonstrate frequent mitochondrial recombination. Importantly, recombination can occur between genetically diverse isolates from distinct taxonomic clades and thus can shed light on genetic exchange between fungal strains.
Cardiorespiratory fitness, body mass index and mortality: a systematic review and meta-analysis
Authors: Weeldreyer Nathan R , De Guzman Jeison C , Paterson Craig , Allen Jason D , Gaesser Glenn A , Angadi Siddhartha S 
Published: November 2024

ObjectiveThe purpose of this review was to assess the joint relationship of cardiorespiratory fitness (CRF) and Body Mass Index (BMI) on both cardiovascular disease (CVD) and all-cause mortality risk.DesignA systematic review and meta-analysis was conducted. Pooled HR and 95% CI were calculated using a three-level restricted maximum likelihood estimation random-effects model with robust variance estimation. The reference group was normal weight-fit and was compared with normal weight-unfit, overweight-unfit and fit, and obese-unfit and fit.Data sourcesElectronic databases (PubMed/MEDLINE, Web of Science and SportDiscus) were searched following registration on PROSPERO.Eligibility criteriaArticles meeting the following criteria were included: (1) published between January 1980 and February 2023, (2) prospective cohort study, (3) CRF assessed using a maximal or VO2peak exercise test, (4) BMI reported and directly measured, (5) joint impact of CRF and BMI on all-cause mortality or CVD mortality were analysed, and (6) the reference group was normal weight, fit individuals.Results20 articles were included in the analysis resulting in a total of 398 716 observations. Compared with the reference group, overweight-fit (CVD HR (95% CI): 1.50 (0.82–2.76), all-cause HR: 0.96 (0.61–1.50)) and obese-fit (CVD: 1.62 (0.87–3.01), all-cause: 1.11 (0.88–1.40)) did not have a statistically different risk of mortality. Normal weight-unfit (CVD: 2.04 (1.32–3.14), all-cause: 1.92 (1.43–2.57)), overweight-unfit (CVD: 2.58 (1.48–4.52), all-cause: 1.82 (1.47–2.24)) and obese-unfit (CVD: 3.35 (1.17–9.61), all-cause: 2.04 (1.54–2.71)) demonstrated 2–3-fold greater mortality risks.ConclusionsCRF is a strong predictor of CVD and all-cause mortality and attenuates risks associated with overweight and obesity. These data have implications for public health and risk mitigation strategies.
Caregiver burden and quality of life in palliative care: cross-sectional study
Authors: Shefeek Suhail K , Joy Teena Mary , Olickal Jeby Jose , Nezrin Malathu Abdulnazer , George Annie , Thankappan Kavumpurathu Raman 
Published: November 2024

BackgroundLiterature on caregiver burden is limited in India and Kerala. We examined the prevalence and factors associated with caregiver burden and its impact on their quality of life.MethodsWe conducted a cross-sectional study among 115 informal caregivers (mean age 70 years, 80% female) of palliative care patients in Kerala. Caregivers were interviewed using the Zarit Burden Interview-22 and the WHOQOL-BREF questionnaires. Factors associated with caregiver burden were analysed using binary logistic regression.ResultsThe prevalence of moderate to severe burden was 32.1% (95% CI 23.7% to 41.5%), with 6.2% reporting severe burden (95% CI 2.4% to 12.1%). Caregivers of patients aged ≥73 years (adjusted OR (aOR)=7.19, 95% CI 1.87 to 27.71, p=0.004), children acting as caregivers (aOR=11.09, 95% CI 1.21 to 101.03, p=0.033) and those caring for patients with prolonged disease duration (aOR=3.62, 95% CI 1.04 to 12.67, p=0.044) reported significantly higher burden compared with their counterparts. Moderate to severe burden was associated with lower physical (p<0.001), psychological (p<0.001) and social relations (p=0.002) quality of life scores.ConclusionTargeted interventions for caregivers of older patients, children acting as caregivers and those taking care of patients with prolonged disease duration are likely to reduce burden and improve their quality of life.
Effect of Protease Combined With Heat Treatment on the Stability of Proteins in Liqueur Wine
Authors: Zhao Yuanyuan , Zhang Zhen , Li Wen , Li Wei , Shang Qi , Du Jianming , Wang Li 
Published: November 2024

Background: Protein instability has been reported to be an important cause of haze in white wines; to investigate the feasibility of protease as a substitute for bentonite in the protein stability of white grape liqueurs, the effect of protease combined with heat treatment on the composition and protein stability of liqueur wines was investigated by chemical analysis, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE), and amino acid analysis.Results: The results showed that the contents of total acid, volatile acid, pH, and glycerol were not significantly different from the blank control; the effect on total protein content and thermal stability were similar to the bentonite treatment; the L∗, b∗, and values of liqueur wines increased by 5.59%, 4.33%, and 4.33% after protease combined with heat treatment (p < 0.05); SDS‐PAGE results confirmed that protease combined with heat treatment had good hydrolysis for thaumatin‐like proteins (TLPs) and chitinases (CHIs) in liqueur wines. Amino acid analysis revealed that the levels of threonine, serine, tyrosine, arginine, and proline had increased, respectively, and isoleucine (1.26 mg/L) and leucine (1.16 mg/L) were newly detected after protease combined with heat treatment. Particle size analysis showed that the protease combined with heat treatment had less effect on the particle size distribution of the wines.Conclusion: Protease combined with heat treatment had a certain protein stabilizing effect and had no significant negative impact on other nutrients of liqueur wines. Thus, it can be used as an alternative to bentonite for the protein stabilization of liqueur wines.
Abstract A015: Validation of a cancer early-detection assay on liquid biopsies using DNA methylation
Authors: Gracheva Ekaterina 
Published: November 2024

Abstract
There is growing awareness of the benefits of early cancer detection. Early intervention can improve patient outcomes, leading to a reduction in the health care burden associated with cancer, and can also provide critical information for personalized treatments. Liquid biopsies enable quick, easy, non-invasive, and real-time testing that is suitable for screening and increases accessibility, particularly in developing countries. The use of epigenomic techniques, such as DNA methylation profiling, enhances the depth and breadth of screening beyond what standard genetic analysis can offer. Universal Diagnostics (UDx), a respected pioneer in the field of early cancer detection, has partnered with Hologic Diagenode, which brings 20 years of experience in epigenomics, to develop a customized early detection workflow for colorectal cancer (CRC). This analysis relies on DNA methylation biomarkers identified within cell-free DNA (cfDNA) from plasma. The UDx assay provides not only easy screening but also a more comprehensive analysis than that possible with conventional markers. Genome-wide methylation profiles from healthy individuals and cancer patients were used to identify biomarkers. These biomarker candidates were then validated for performance; the results of which are presented in this poster.
Citation Format: Ekaterina Gracheva. Validation of a cancer early-detection assay on liquid biopsies using DNA methylation [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A015.
Abstract A021: Analytical platform to validate microRNA cancer biomarkers
Authors: Kanavarioti Anastassia , Rehman M. Hassaan , Qureshi Salma , Rafiq Aleena , Sultan Madiha 
Published: November 2024

Abstract
We developed and tested a novel assay to quantify and validate microRNA (miRNA) cancer biomarkers in liquid biopsies. The assay is amplification-free, exploits a commercially available nanopore-array device for detection (MinION from Oxford Nanopore Technologies), uses total RNA isolated from biospecimen as the miRNA source, and proprietary osmylated oligonucleotides, complementary to the target miRNA, as probes. The assay exploits a bracketing experimental protocol to quantify miRNA, and yields miRNA copy numbers (copies) per 1microLiter of isolated total RNA, with an unprecedented accuracy of approximately +/- 20% across all concentrations. For development purposes and as a control/reference the combined serum of healthy men (# H6914 from Sigma-Aldrich) was used. During a period of 4 years different lots of H6914 yielded, within experimental error, comparable miRNA copies for let-7b, miR-16, miR-21, miR-15b, miR-375 and miR-141. Serum samples from healthy individuals and cancer patients were also tested. Once data were normalized to the same total RNA content, miRNA copies appeared to belong to one of two groups. The first group included miR-21, miR-375 and miR-141 copies in H6914 and in healthy samples. The second group included miR-21, miR-375 and miR-141 copies in cancer samples exhibiting an approximate 1.8-fold overexpression compared to the first group. miR-15b copies in H6914, healthy and cancer samples were all in the first group. The two groups exhibited zero data overlap which is unprecedented in the miRNA/cancer literature. Zero data overlap was the result of (i) the assay’s bracketing approach with a protocol-defined accuracy of approximately +/-20%, and (ii) the normalization of miRNA copies to the same total RNA content. Notably, these two groups included both serum and urine samples from cancer patients and healthy individuals. All subjects were deselected regarding age, sex, and ethnicity, while patients were selected for breast, prostate or pancreatic cancer, Stage I or II, pretreatment. To the best of our knowledge, this is the first-time agreement between serum and urine miRNA copies is reported and leads to the suggestion that a urine sample may replace a blood draw for the tested miRNAs. Not only clear discrimination between healthy and cancer samples was possible, but this analytical platform may be used to validate tentative cancer/disease biomarkers when miRNA overexpression between cancer/disease and healthy is equal or higher than 1.8-fold. Non-Coding RNA 2024, 10(4), 42; https://doi.org/10.3390/ncrna10040042
Citation Format: Anastassia Kanavarioti, M. Hassaan Rehman, Salma Qureshi, Aleena Rafiq, Madiha Sultan. Analytical platform to validate microRNA cancer biomarkers [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A021.
Abstract A033: Leveraging mass cytometry for phenotyping CTCs in SCLC liquid biopsies: Tracking therapy resistance at a personalized level
Authors: Karacosta Loukia G , Bhattacharyya Sayantan , Stewart Allison , Victorian Ashley , Lujan Ester F , Ehsan Shafqat , Kim Subin , Duarte Alberto , Wang Runsheng , Anchang Benedict , Wang Jing , Byers Lauren 
Published: November 2024

Abstract
This abstract is being presented as a short talk in the scientific program. A full abstract is printed in the Proffered Abstracts section (PR005) of the Conference Program/Proceedings.
Citation Format: Loukia G Karacosta, Sayantan Bhattacharyya, Allison Stewart, Ashley Victorian, Ester F Lujan, Shafqat Ehsan, Subin Kim, Alberto Duarte, Runsheng Wang, Benedict Anchang, Jing Wang, Lauren Byers. Leveraging mass cytometry for phenotyping CTCs in SCLC liquid biopsies: Tracking therapy resistance at a personalized level [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A033.
Abstract A047: Detecting Hypoxic Circulating Tumor Cells (H-CTC) with a novel technique
Authors: Zayas Beatriz , Rios Karoline , Cox Osvaldo 
Published: November 2024

Abstract
There is a continues need to develop technologies to detect and characterize circulating tumor cells that can become aggressive, metastatic, and hard to treat. Among these aggressive CTC are cells known as Hypoxic cell populations that learn to adapt and survive to low oxygen levels. Our project aims to test the applicability of a novel technique to detect Hypoxic Circulating Tumor Cells (H-CTC) that can move from tumors to circulating blood invading other tissues and becoming resistant to chemotherapy and radiation. The proposed assay incorporates a set of patented organic compounds as markers of hypoxic circulating tumor cells (H-CTC) with potential clinical applications. To date, our research has generated preliminary in-vitro studies with a novel NBQ-TOM demonstrating its capacity as markers of hypoxic human cancer cells in vitro. Comparison with the control Pimonidazole demonstrated the advantages of novel NBQ- TOM compounds as hypoxic marker. To determine the capacity of NBQ-TOM as hypoxic fluorescent marker, samples of 15 ml human whole blood were spiked with hypoxic or normoxic colon cancer cells (COLO 205). Spiked hypoxic or normoxic cells are then enriched, recovered, and treated with NBQ-TOM (25uM) for 24 hours. After treatment generated fluorescence is measured with an OPTIMA BMG Fluorimeter to demonstrate the significant formation of a fluorescent metabolite on hypoxic tumor cells in contrast to cells treated under normoxic environment. Also, fluorescence microscopy analysis confirmed the stronger fluorescence generation at hypoxic conditions. These preliminary results strongly support the applicability of these molecules for an in-vitro detection assay of hypoxic CTC in circulating blood that can become metastatic. Additional sensitivity and specificity analysis needs to be implemented.
Citation Format: Beatriz Zayas, Karoline Rios, Osvaldo Cox. Detecting Hypoxic Circulating Tumor Cells (H-CTC) with a novel technique [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A047.
Abstract A048: Screening early detection of HPV-associated oropharynx cancers with multi- feature HPV whole genome sequencing liquid biopsy
Authors: Kuhs Krystle , Robbins Hilary , Das Dipon , Bryan Michael , Aye Ling , Al-Inaya Yana , Hirayama Shun , Adalsteinsson Viktor , Lawrence Michael , Waterboer Tim , Faden Daniel L 
Published: November 2024

Abstract
This abstract is being presented as a short talk in the scientific program. A full abstract is printed in the Proffered Abstracts section (PR007) of the Conference Program/Proceedings.
Citation Format: Krystle Kuhs, Hilary Robbins, Dipon Das, Michael Bryan, Ling Aye, Yana Al-Inaya, Shun Hirayama, Viktor Adalsteinsson, Michael Lawrence, Tim Waterboer, Daniel L Faden. Screening early detection of HPV-associated oropharynx cancers with multi- feature HPV whole genome sequencing liquid biopsy [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A048.