bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis, antibacterial and antifungal activity of 1-[4-(1,1,3,3-tetramethylbutyl)phenoxy]-3-dialkylamino-2-propanol quaternary salts
Authors: Korotkii Y. V. , Vrynchanu N. A. , Dronova M. L. , Suvorova Z. S. , Smertenko O. A. 
Published: September 2019

The emergence and spread of resistant strains of pathogens as well as reduction of the efficacy of current antimicrobial agents requires the development of novel antimicrobial compounds.
The aim of the present study was synthesis and evaluation of antimicrobial activity of new arylaliphatic aminopropanols.
The objects of the present study were 1-[4-(1,1,3,3-tetramethylbutyl)phenoxy]-3-dialkylamino-2-propanol quaternary salts (compounds I–XIV).
Compounds synthesis was carried out by heating of precursor epoxide and excessive amount of appropriate amines in isopropanole, followed by treatment with excess of alkyl halides. Methods of elemental analysis, IR- and PMR-spectroscopy were used for confirmation of chemical structure. Antimicrobial activity against Staphylococcus аureus АTCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa АТСС 27853 and Candida albicans NCTC 885/653 was determined by a broth dilution method and evaluated via minimum inhibitory concentration (MIC).
Our investigation of antibacterial and antifungal activity of 1-[4-(1,1,3,3-tetra methylbutyl)phenoxy]-3-dialkylamino-2-propanol quaternary salts showed that compounds possess narrow spectrum, as well as broad spectrum action. Significant antimicrobial activity of the novel aryl aliphatic aminoalcohols indicates their potential usage as a component of new antimicrobial drugs.
Structural and adsorptive properties of silica-alumina and clay materials of mineral and synthetic origin
Authors: Gerashchenko I. I. , Voitko I. I. , Vasilieva A. V. 
Published: September 2019

Location on territory of Ukraine of great deposits of aluminosilicates together with good facilities for their processing and standardization promotes the study of this raw material, results of which could be useful for the developers of enterosorbents. For search the specific sorptive activity a comparative examination of several materials of silica-alumina and clay nature has been carried out in this work.
The X-ray phase analysis, a measuring of specific surface area by the low temperature adsorption–desorption of nitrogen and the method of isotherm of adsorption have been used.
It is shown that silica-alumina and clay materials have much less specific surface as compared to silica gel Syloid® 244 FP and carbon sorbent SCN. The sorbents of montmorillonite type (smectite, saponite) are characterized by the highest adsorptive capacity in relation to methylene blue and congo red dyes. Synthetic zeolites NaA and NaX effectively absorb the ions of zinc from solution, significantly overcoming Syloid® 244 FP and SCN which were taken as preparations of comparison. Therefore, on the basis of NaA and NaX the selective enterosorbents for elimination from the organism of toxic metals could be created.
Evaluation of factions antigens disintegration fungal cells Candida albicans and Candida tropicalis if advised candidiasis
Authors: Rybalkin M. V. , Filimonova N. I. , Strilets O. P. , Strelnikov L. S. 
Published: September 2019

Candidiasis – one of the most common fungal diseases humanity has ever faced. Etiological factors of the disease are most often Candida albicans and Candida tropicalis. To fight Candida infection has been actively conducting research on vaccine development, as in the CIS countries and in the countries of Europe and America. In the context of this research have shown trends of development of combined subunit vaccines based on the fungi Candida. Authors at the National University of Pharmacy at the Department of Biotechnology and Microbiology, Virology and Immunology developed a method of disintegration of cells of fungi C. albicans and C. tropicalis using ultrasound.
The objective of our work was the experimental evaluation of fungal cell antigen fractions C. albicans and C. tropicalis in the prevention and treatment of candidiasis.
Fungal cells from C. albicans and C. tropicalis were isolated proteins and polysaccharides using an ultrasonic disintegrator when extending wave of 22 kHz and 15 min exposure. Filtered through a membrane «Vladipor» IPA-MA № 3, which provides shutoff of the biological material with a size of 10 kDa.Obektom study were fractions dezintegrata fungal cell C. albicans and C. tropicalis molecular size and greater than 10 kDa. Mice were intramuscularly twice at an interval of 14 days were injected into the upper portion of the right hind paw of 0.2 ml of separately investigated fractions at a protein concentration for C. albicans 3 mg/ml for C. tropicalis 5 mg/ml. After 1 month, for one group, and after 3 months for a second group of test animals after the second injection, the animals carried abdominal infection. After 14 days the animals were carried out inspection and measured results
The studies found that the fraction of cells dezintegrata fungi C. albicans and C. tropicalis with a molecular size of 10 kDa at double intramuscular injection of 0.2 ml provides 100% efficacy in the prevention of Candida infections.
Synthesis of new potential biological active substances among 3-mercapto-4-amino-5-benzyl-1,2,4-triazol-4H derivatives
Authors: Saidov N. B. , Georgiyants V. A. , Garnaya N. V. 
Published: September 2019

Conventional approaches to the formation of 1,2,4-triazole ring allow to introduce different substituents into the heterocyclic ring during the synthesis of key intermediates stage. In continuation of previous studies the synthesis of 3-mercapto-4-amino-5-benzyl-1,2,4-triazole-4H derivatives is described.
The aim of investigation was synthesis of a series of new derivatives of 3-mercapto-4-amino-5-benzyl-1,2,4-triazole, proving their structure by physico-chemical methods and planning of pharmacological screening using PASS.
Phenylacetic acid ethyl ester has been used for the synthesis as starting substance. It was subjected to hydrazinolysis to receive an appropriate hydrazide. In the next stage substance obtained was processed with hydrogen disulfide and appropriate potassium ditiokarbaminat has been received, which by reaction with an excess of hydrazine hydrate has been cyclised into a target 3-mercapto-4-amino-5-benzyl-1,2,4-triazol-4H. The first stage of chemical transformation reactions of key intermediate was its alkylation with chloroacetanilides under alkaline catalysis. By Paal–Knorr reaction the amino group in the synthesized alkylthio-4-amino-5-benzyl-1,2,4-triazole-4H was modified into pyrrole ring by the action of 2,5-dimethoxytetrahydrofuran in acetic acid medium.
The structure of the compounds synthesized has been proved by 1H NMR spectroscopy. The successful alkylation of mercapto group indicates the disappearance of its proton signal at the spectra and appearance of the acetanilide residues protons signals. Modification of the amino derivatives into corresponding pyrrolyl substituted has been accompanied by the appearance of the triplet signals of magnet equivalent methyne protons of the pyrrole ring (protons at positions 3.4) at 6.30 ppm. The signals of the second pyrrole protons pair overlap with other aromatic protons signals.
For the planning of pharmacological screening previous prognosis of the potential pharmacological activity spectrum has been carried out using software PASS. The prognosis showed that for all pyrrolyl substituted substances predominant in the spectrum of action was the influence on the central nervous system, and the probability of the activity of their synthetic precursors mainly limited to moderate antioxidant properties.
A relational database with pharmaceutical information: problems of creation and primary filling for provision of a qualitative statistical processing
Authors: Rervyatskyy I. Yu. 
Published: September 2019

The disclosure of pharmaceutical information is not intended to provide the possibility of further automated processing of the data provided by independent experts, and, accordingly, the choice of the methods of presentation is aimed to optimize visual perception by users.
The aim of the work was to analyze in the internet-sources the availability of information in the appropriate format for automatic filling of the relational database. The subjects of the study were: ATC: ICD-10 (International Classification of Diseases); content of EF 9.8; classification of dosage forms by the Ministry of Health of Ukraine, FDA (Food and Drug Administration, USA), EMA (European Medicines Agency) and EphMRA (European Pharmaceutical Market Research Association). Methods used are review of information with the corresponding structure in the internet, processing of the information found using computer code.
Approbation was carried out on the basis of the digital online system «Likypedia» (http://likypedia.zzz.com.ua; http://facebook.com/likypedia).
The basic goals for the quality of the results of statistical processing of pharmaceutical information are formed. To achieve them, a list of required information for the initial download to the relational database is defined. The sources of the received information which was used for initial loading to the relational database are presented. The multicomponent record of pharmaceutical information is described.
The list of dosage forms was formed on the basis of own practical experience, trends in the indication on packaging of medicines by manufacturers, electronic databases of pharmaceutical wholesalers, the titles of articles given in the EF 9.8 edition. Information from different sources about pharmaceutical dosage forms was analyzed and divided into five information blocks: made by manufacturer; its characteristic; for DF, which will be prepared with DF produced by the manufacturer; its characteristic; way of using DF.
A variant has been developed and presented for structuring the information record of the drug dose in the relational database in several variants, which makes it possible to further carry out automated calculations, optimize the selection and sorting of data.
In silico screening of drug-like molecules for the treatment of cardiovascular diseases on the basis of five-membered privileged heterocycles
Authors: Drapak І. V. 
Published: September 2019

Among various heterocyclic systems, the derivatives of five-membered heterocycles are of special interest. Most of the above mentioned heterocycles are treatred as so-called privileged structures in modern medicinal chemistry. In silico screening among five-membered heterocycles of molecules for the treatment of cardiovascular diseases is actual.
The aim of the work was the search for synthetic drug-like molecules based on functionalized five-membered heterocycles and related heterocyclic systems as an element of the theoretical platform for rational design of compounds acting on the cardiovascular system, and prediction of their possible mechanism of action.
The objects of the study were derivatives of uncondensed and condensed five-membered heterocycles. In the work, in silico approaches were applied using the programs: Hyper-Chem, PASS, AutoDock, PROTOX.
Based on the previous studies, focused sub-libraries of small synthetic drug-like molecules based on functionalized five-membered heterocycles and related heterocyclic systems have been selected. The compounds were divided on 12 groups. The optimization of the compound structures, the drug-like parameters calculation were carried out. The activity prediction, the acute toxicity level and docking studies to probable bio-targets which are related with cardiovascular drug mechanism of action have been carried out. It was shown that thiazole and thiadiazole based compounds possessed the highest calculated affinity levels to selected bio-targets. This is consistent with PASS-based prediction data.
Diverse functionalized derivatives of five-membered heterocycles (thiazole, thiazolidine, thiadiazole, pyrazole, thiophene, triazole) and related fused heterocycles have been grouped in focused sub-libraries of compounds. it has been established that thiazole and thiadiazole based compounds are promising objects for directed synthesis and further modification as potential cardiovascular agents based on the prediction of biological activity, the calculation of affinity to potent bio-targets, and the prediction of the drug-like features and acute toxicity level. The prognostic values of the parameters of the above mentioned groups of compounds may be used as the element of theoretical platform for the search and de novo design of potential drugs for the treatment of cardiovascular diseases.
Luminescent determination of residues of trazodon hydrochloride and melatonin after cleaning pharmaceutical equipment
Authors: Voitiuk O. D. , Yegorova A. V. , Scrypynets Yu. V. , Kashutskуy S. N. , Antonovich V. P. 
Published: September 2019

A prerequisite for ensuring the quality of medicines is their production in accordance with the rules of GMP (Good Manufacturing Practice for Medicinal Products), one of the most important requirements of which is equipment cleaning. In many cases, the same equipment is used in the production of various preparations. Therefore, to prevent contamination of each of the following drugs, the previous one, it is very important to carry out an effective equipment cleaning procedure with a mandatory assessment of its purity.
The purpose of this study was to develop simple, express, selective methods for luminescent determination of residual quantities of APIs of trazodone hydrochloride (TG) and melatonin (MT) in washes to control the completeness of their removal when cleaning process equipment.
The excitation and luminescence spectra were recorded using a Cary Eclipse "Varian" spectrofluorimeter (Australia) with a xenon lamp 150 W. Electronic absorption spectra were recorded on a UV-2401 PC spectrophotometer «Shimadzu» (Japan).
The electronic absorption spectra of TG and MT have absorption bands in the UV spectral region. It was established experimentally that the excitation spectra of TG and MT are similar to their absorption spectra (λex = 318 nm (TG) and λem = 274 nm (MT)). The effect on the luminescence intensity of TG and MT of methanol, ethanol, acetonitrile, dimethylformamide, dimethylsulfoxide, propanol (50 v/v) was studied. It is established that the maximum luminescence is observed in water.
The methods were validated according to the following parameters: specificity, linearity, accuracy, limit of quantitation. The degree of extraction of trazodone hydrochloride and melatonin from applicators and surfaces of pharmaceutical equipment is more than 90%. The developed methods can be recommended for determining the residual amounts of trazodone hydrochloride and melatonin while monitoring the quality of the cleaning of pharmaceutical equipment.
The research of anti-inflammatory activity of 5-(5-bromofuran-2-yl)-4-ethyl-1,2,4-triazole-3-thion S-derivatives
Authors: Pruglo Ye. S. 
Published: September 2019

The studing of anti-inflammatory activity of the novel compounds may give rise to the using of safer and more active drugs in the treatment of diseases which are shown NSAIDs.
To further study the pharmacological properties of the synthesized c compounds were studied for acute toxicity and anti-inflammatory activity of 5-thio derivates of 3-(5-вromofuran-2-yl)-4-ethyl-(4H)-1,2,4-triazole at white rats by formalin method of acute edema.
After investigation of anti-inflammatory activity in vivo of first synthesized 1,2,4-triazole derivatives it was found that potassium salt of 2-(4-ethyl-5-(5-bromfuran-2-yl)-1,2,4-triazoles 3-iltio) acetic acid (Compound 24) had the most significant anti-inflammatory activity. This compound suppressed the formation of edema quotes of rats at 59.87% and was more active than the reference drug diclofenac at 15.13%.
There were established the patterns regarding chemical structure and pharmacological action of these substances. So replacing of bromine atom at the dimethylamino group in the para-position of the phenyl radical in the molecule of N'-(4-brombenzyliden)-2-((5-(5-bromfuran-2-yl)-4-ethyl-4H-1,2,4-triazole-3-yl)tio)acetic hydrazid (Compound 36) was accompanied by appearence of the anti-inflammatory action.
It was revealed that in the number from the ammonium salt (Compound 30) to monoethanolamonium salt (Compound 23) and to the morpholinum salt (Compound 22) it was observed an increase of inflammation and probably growing number of pro-inflammatory mediators released.
Relationship of antimicrobial activity and chemical structure of the arylaliphatic aminoalcohol derivatives
Authors: Dronova M. L. , Suvorova Z. S. , Korotkyi Yu. V. 
Published: September 2019

Arylaliphatic aminoalcohols appeared to be the new promising class of compounds for the development of antibacterial and antifungal agents. The aim of the presented study was to analyze the «chemical structure-antimicrobial activity» relationship for further activity-directed synthesis of compounds of this class.
The antimicrobial activity of the compounds was investigated by serial broth dilution method. Primary analysis of the effect of substituents’ structure on the ability of the derivatives to inhibit the growth of test-microorganisms was carried out by empirical method. Molecular structural characteristics of arylaliphatic aminoalcohols (surface area, volume, partition coefficient logP and dipole moment) were calculated by means of «Hyperchem 8.0.8» software. Relationship between the antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and molecular characteristics was investigated by correlation analysis using the Spearman test. Statistical processing was performed by «StatSoft Statistica 6.0», the data obtained were evaluated using Chaddock scale.
The data obtained suggest, that the presence of both antibacterial and antifungal activity in arylaliphatic aminoalcohols depends on the amino group structure and composition of aryl(alkyl)oxy-radical (4-(1,1,3,3-tetramethylbutyl)phenyl, 1-adamantyl, 4-(1-adamantyl)phenyl, 4-phenyl-phenyl or 2,4-ditretbutyl phenyl). The correlation analysis revealed an inverse relationship between the antimicrobial action and surface area, volume, and lipophilicity of compounds. The tightest correlation was found between these parameters and antistaphylococcal activity. Our results indicate the promises of the synthesis, directed to the reducing of the molecule size and lipophilicity of tetramethylbutylphenyl aminoalcohols, for further development of broad-spectrum antimicrobial agents.
A comparative study of nature of adnexal masses by ultrasonography and histopathology
Authors: Roshed Md Mahabubur , Akhter Mst Dalia , Hossain Syed Mozammel 
Published: September 2019

Background: Adnexal masses are common lesions in women across the globe. This is also important for woman in Bangladesh. The incidence of adnexal malignancy ranks only after the carcinoma of cervix and endometrium. So their accurate and early diagnosis is necessary for design management and treatment protocol.
Objective: The aim of the study is to assess the correlation between histopathological examination and ultrasonographic evaluation in making early and confirm diagnosis of adnexal masses.
Methodology: Eighty four women with different ages having adnexal mass, diagnosed by ultrasonographic evaluations and followed by surgery were included in this study between january 12 to December, 14. The morphologic characteristics of the masses were evaluated with gray scale Ultrasonography (USG). Specimens were collected after surgical resection in a container containing 10% formalin. The specimens were processed by paraffin embedding method and stained by routine Haematoxylin and Eosin stain for microscopic examination. The results of this study were calculated by standard statistical formula.
Results: Histopathological examination revealed that 68 masses (80.95%) were benign, two (2.38%) were borderline malignant, and 16 (19.05%) were malignant. USG enabled correct diagnosis of 63 of the 68 benign masses (92.65%) and all 16 malignant masses (100%).
Conclusion: There is a correlation between histopathological examination and ultrasonographic evaluation of adnexal masses in most cases. Therefore the imaging analysis of internal architectural appearance by USG and histopathological examination increases the specificity in the diagnosis of adnexal masses.
Bang Med J (Khulna) 2018; 51 : 7-11
Variation of blood pressure during change of posture
Authors: Islam Md Nazrul , Kamal SK , Islam Md Sirajul , Hossain Sk Amir 
Published: September 2019

Background: Many factors can influence blood pressure, one of which is body position. The aim of present study was to establish a specific pattern of blood pressure (BP) changes in accordance with posture changes in healthy adults.
Objective: Aim of present study was to establish a specific pattern of blood pressure (BP) changes in accordance with posture changes in healthy adult male and females.
Methods: Blood pressure was recorded by aneroid sphygmomanometer in supine, sitting and standing posture on both arms. Mean ± standard deviation of the observation for all the parameters were calculated and comparison in supine, sitting and standing position was done by repeated measures Analysis was done by ANOVA test and tukey's post hoc test.
Results: Fifty healthy males and fifty healthy female took part in this study. BP varies according to body position. Both Systolic blood pressure and diastolic blood pressure was significantly decreased (P<0.05) while changing posture from supine to standing. But blood pressure changes during change of posture from supine to sitting or from sitting to standing was not significant (P>0.05).
Conclusions: Change in body posture changes systolic blood pressure as well as diastolic blood pressure.
Bang Med J (Khulna) 2018; 51 : 16-20
Comparison between flunarizine and levetiracetam in paediatric migraine prophylaxis
Authors: Rahman Sk Masiur , Kundu Gopen Kumar , Fatema Kanij , Akhter Shaheen , Rahman Md Mizanur 
Published: September 2019

Background: Migraine hampers child's life through school absence, limitation of home and social activities. The goal of prophylactic (preventive) therapy is to reduce headache frequency, headache days, and headache severity with minimum side effects.
Objective: To evaluate the efficacy and tolerability of levetiracetam compared to flunarizine as prophylactic treatment of Migraine in children. Methods: This prospective study was carried out in the outpatient department of a tertiary care hospital in Bangladesh. A total of 105 children aged 6-15 years, diagnosed as migraine without aura, were enrolled. Patients were treated with flunarizine or levetiracetam for three months. Headache disabilities were evaluated at baseline and at the end of three months.
Results: In flunarizine group, 54 children and in levetiracetam group, 51 children were enrolled. Among them thirty nine children in flunarizine group and 36 children in levetiracetam group completed the study. Headache frequency, headache days, VAS (Visual Analogue Scale) score and PedMIDAS (Paediatric Migraine Disability Assessment Score) score were evaluated at baseline and during the follow up. After three months, headache frequency, headache days, pain severity (Visual Analogue Scale score) reduced significantly compared to baseline (p<0.05). PedMIDAS score was also reduced from 60.35(±16.36) to 30.13(±14.28) in flunarizine group and from 64.25(±19.63) to 25.91(±18.6) in levetiracetam group (p<0.05). Some minor side effects were also reported by both groups, but were well tolerated by the patients and need not withdrawal of medication.
Conclusion: Levetiracetam is as effective as flunarizine in paediatric Migraine Prophylaxis.
Bang Med J (Khulna) 2018; 51 : 35-39