bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Caribbean and Farallon Plates Connected: Constraints From Stratigraphy and Paleomagnetism of the Nicoya Peninsula, Costa Rica
Authors: Boschman L. M. , van der Wiel E. , Flores K. E. , Langereis C. G. , van Hinsbergen D. J. J. 
Published: June 2019

AbstractPlate kinematic reconstructions play an essential role in our understanding of global geodynamics, but become increasingly difficult to constrain back in geological time due to the subduction of oceanic lithosphere. Here, we attempt to kinematically reconstruct the Cretaceous and older plate tectonic history of the Caribbean Plate within the Mesozoic Panthalassa (paleo‐Pacific) Ocean. To this end, we present new paleomagnetic data from Jurassic and Cretaceous oceanic sedimentary and volcanic Large Igneous Province‐related rocks of the Nicoya Peninsula and Murciélago Islands of northwestern Costa Rica. We use these data, in combination with constraints from marine magnetic anomalies to infer the age of the lithospheric basement, seismic tomography to locate deep‐mantle plume generation zones, and general kinematic feasibility, to test different reconstruction scenarios connecting the Caribbean Plate to the Farallon Plate as restored from Pacific spreading records. Our resulting reconstruction implies that the western Caribbean subduction zone initiated around 100 Ma, in an intraoceanic setting, breaking up oceanic lithosphere of at least 70 Myr old.
Micromechanics of High‐Pressure Compaction in Granular Quartz Aggregates
Authors: Hangx Suzanne J. T. , Brantut Nicolas 
Published: June 2019

AbstractThe mechanical behavior of porous sandstones is generally modeled using concepts from granular mechanics, often overlooking the effect of cementation. To probe the key differences between sand and sandstone mechanics, we performed triaxial deformation experiments on Ottawa quartz sand at 5‐ to 40 MPa effective confining pressure. At 5 MPa, the samples are able to dilate. At higher confinement, the aggregates show continuous compaction, displaying strain hardening. The stress‐strain behavior is nonlinear, and the exact onset of inelastic compaction could not be determined accurately. Measured P‐wave velocities show the development of anisotropy. With increasing axial strain, the along‐axis velocities tend to increase, while velocities perpendicular to the compression axis tend to decrease (at low pressure) or remain constant (at high pressure). In samples deformed under elevated pressure conditions, acoustic emission event locations are diffuse. Microstructural investigations show an increase in grain chipping and crushing with increasing confining pressure, but no evidence of localized compaction could be observed. The nature of the pore fluid, either decane or water, does not significantly influence the mechanical behavior at strain rates of 10−6 to 10−4 s−1. Grain angularity and grain‐size distribution also did not significantly change the mechanical behavior. We infer that our observations indicate that the lack of cementation introduces additional degrees of freedom for grains to slide, rotate, and reorganize at the sample scale, precluding the existence and sustainability of stress concentrations beyond the grain scale. This results in progressive compaction and hardening, and lack of compaction localization.
Generation of Seed Electrons by Extensive Air Showers, and the Lightning Inception Problem Including Narrow Bipolar Events
Authors: Rutjes Casper , Ebert Ute , Buitink Stijn , Scholten Olaf , Trinh Thi Ngoc Gia 
Published: June 2019

AbstractLightning streamers and leaders need thermal electrons to initiate, but free electrons are extremely rare in the wet air of a thundercloud. Here we analyze the probabilities that high electron densities occur in extensive air showers. We argue that relevant air showers are created by cosmic particles with energies between 1015 and 1017 eV impinging onto our atmosphere. We simulate a large number of air showers and perform a stochastic analysis of their results. We present the available densities of thermal electrons as a function of altitude, time interval, and considered area, while neglecting effects of local electric fields. We find that free electron densities at altitudes between 5 and 13 km can reach values of order 103 cm−3, but only in shower cores with a radius on a centimeter scale. Above 6 km, the availability of extreme free electron densities decreases significantly with increasing altitude. Recent measurements by Rison et al. (2016, https://doi.org/10.1038/ncomms10721) indicate that several streamers must have been triggered simultaneously during discharge inception, and we suggest that an extensive air shower could have been the trigger. Rison's measurements show further that the streamers are laterally separated by several tens of meters, so they must have been triggered by electron densities as low as 1 cm−3. Such low electron densities demand a stochastic approach to streamer initiation near hydrometeors.
Spatial Modeling of Local‐Scale Biogenic and Anthropogenic Carbon Dioxide Emissions in Helsinki
Authors: Järvi Leena , Havu Minttu , Ward Helen C. , Bellucco Veronica , McFadden Joseph P. , Toivonen Tuuli , Heikinheimo Vuokko , Kolari Pasi , Riikonen Anu , Grimmond C. Sue B. 
Published: June 2019

AbstractThere is a growing need to simulate the effect of urban planning on both local climate and greenhouse gas emissions. Here, a new urban surface carbon dioxide (CO2) flux module for the Surface Urban Energy and Water Balance Scheme is described and evaluated using eddy covariance observations at two sites in Helsinki in 2012. The spatial variability and magnitude of local‐scale anthropogenic and biogenic CO2 flux components at high spatial (250 m × 250 m) and temporal (hourly) resolution are examined by combining high‐resolution (down to 2 m) airborne lidar‐derived land use data and mobility data to account for people's movement. Urban effects are included in the biogenic components parameterized using urban eddy covariance and chamber observations. Surface Urban Energy and Water Balance Scheme reproduces the seasonal and diurnal variability of the CO2 flux well. Annual totals deviate 3% from observations in the city center and 2% in a suburban location. In the latter, traffic is the dominant CO2 source but summertime vegetation partly offsets traffic‐related emissions. In the city center, emissions from traffic and human metabolism dominate and the vegetation effect is minor due to the low proportion of vegetation surface cover (22%). Within central Helsinki, human metabolism accounts for 39% of the net local‐scale emissions and together with road traffic is to a large extent responsible for the spatial variability of the emissions. Annually, the biogenic emissions and sinks are in near balance and thus the effect of vegetation on the carbon balance is small in this high‐latitude city.
Integrating Species‐Specific Information in Models Improves Regional Projections Under Climate Change
Authors: Remy Cécile C. , Krofcheck Dan J. , Keyser Alisa R. , Litvak Marcy E. , Collins Scott L. , Hurteau Matthew D. 
Published: June 2019

AbstractModels commonly used to project forest carbon response to climate change reduce biodiversity to a small number of plant functional types or plant functional traits for the sake of computational efficiency at large spatial scales. We simulated the climate sensitivity of the dominant woody vegetation types in New Mexico using both a generalized functional type and a species‐specific model parameterization. Both parameterizations achieve reasonable current carbon uptake rates and aboveground biomass amount at the ecosystem scale. When vegetation types are subjected to increasing temperature and decreasing precipitation, the generalized parameterization differs substantially from the species‐specific parameterization by homogenizing the diversity of adaptations for dealing with higher temperature and drought, leading to divergent responses under changing climate. We recommend integrating species‐specific information, when available, to improve projections of climate change impacts on forested ecosystems to develop robust ecosystem management strategies at regional scales.
The Energy Side of Budyko: Surface‐Energy Partitioning From Hydrological Observations
Authors: Yin Jun , Calabrese Salvatore , Daly Edoardo , Porporato Amilcare 
Published: June 2019

AbstractLand‐surface partitioning of net radiation into sensible and latent heat fluxes is critical for hydroclimatic processes but remains highly uncertain due to limited observations. We show that a suitable extension of the Budyko's curve, a well‐known framework in hydrology for water balance estimation, can be utilized effectively to partition the surface energy fluxes by expressing the long‐term evaporative fraction (EF) as a function of the dryness index only. The combination of this energy partitioning method with hydrological observations allows us to estimate the surface energy components at watershed and continental scales. Using this new framework, we show that North American Regional Reanalysis data overestimate surface evaporation, likely influencing the modeling of atmospheric convection. The obtained hydrologic constrains on energy partitioning can be used to provide more accurate estimations of surface energy fluxes for hydroclimatic predictions.
Dissolution Dominates Silica Cycling in a Shelf Sea Autumn Bloom
Authors: Poulton Alex J. , Mayers Kyle M.J. , Daniels Christopher J. , Stinchcombe Mark C. , Woodward E. Malcolm S. , Hopkins Joanne , Wihsgott Julianne U. , Widdicombe Claire E. 
Published: June 2019

AbstractAutumn phytoplankton blooms represent key periods of production in temperate and high‐latitude seas. Biogenic silica (bSiO2) production, dissolution, and standing stocks were determined in the Celtic Sea (United Kingdom) during November 2014. Dissolution rates were in excess of bSiO2production, indicating a net loss of bSiO2. Estimated diatom bSiO2contributed ≤10% to total bSiO2, with detrital bSiO2supporting rapid Si cycling. Based on the average biomass‐specific dissolution rate (0.2 day−1), 3 weeks would be needed to dissolve 99% of the bSiO2present. Negative net bSiO2production was associated with low‐light conditions (<4 E·m−2·day−1). Our observations imply that dissolution dominates Si cycling during autumn, with low‐light conditions also likely to influence Si cycling during winter and early spring.
STYK1 promotes tumor growth and metastasis by reducing SPINT2/HAI-2 expression in non-small cell lung cancer
Authors: Ma Zhiqiang , Liu Dong , Li Weimiao , Di Shouyin , Zhang Zhipei , Zhang Jiao , Xu Liqun , Guo Kai , Zhu Yifang , Han Jing , Li Xiaofei , Yan Xiaolong 
Published: June 2019

AbstractNon-small cell lung cancer (NSCLC) is the leading cause of cancer deaths worldwide. However, the molecular mechanisms underlying NSCLC progression remains not fully understood. In this study, 347 patients with complete clinicopathologic characteristics who underwent NSCLC surgery were recruited for the investigation. We verified that elevated serine threonine tyrosine kinase 1 (STYK1) or decreased serine peptidase inhibitor Kunitz type 2 (SPINT2/HAI-2) expression significantly correlated with poor prognosis, tumor invasion, and metastasis of NSCLC patients. STYK1 overexpression promoted NSCLC cells proliferation, migration, and invasion. STYK1 also induced epithelial–mesenchymal transition by E-cadherin downregulation and Snail upregulation. Moreover, RNA-seq, quantitative polymerase chain reaction (qRT-PCR), and western blot analyses confirmed that STYK1 overexpression significantly decreased the SPINT2 level in NSCLC cells, and SPINT2 overexpression obviously reversed STYK1-mediated NSCLC progression both in vitro and in vivo. Further survival analyses showed that NSCLC patients with high STYK1 level and low SPINT2 level had the worst prognosis and survival. These results indicated that STYK1 facilitated NSCLC progression via reducing SPINT2 expression. Therefore, targeting STYK1 and SPINT2 may be a novel therapeutic strategy for NSCLC.
Is the population of Sado Island genetically close to the population of western Japan?
Authors: Misawa Kazuharu , Watanabe Hiroshi , Yokoseki Akio , Wakasugi Minako , Onodera Osamu , Narita Ichiei , Momotsu Takeshi , Sato Kenji , Endo Naoto 
Published: June 2019

AbstractTo explore the effect of aging, a cohort study is being performed on Sado Island, which is located in the Sea of Japan. Sado Island is close to the eastern coast of Japan, yet its population speaks the western Japanese dialect. Consequently, the genetic background of the population of Sado Island is of interest. Based on Nei’s genetic distance, we compared the allele frequencies of people from Sado Island to those of people from Nagahama and Miyagi, which are located in the western and northeastern parts of Honshu, respectively. The results showed that the populations of Miyagi and Nagahama are genetically closer to each other than to the population of Sado Island. Because the Sado and Honshu Islands are isolated by a channel, it is possible that genetic drift occurred within Sado Island, which would explain the uniqueness of the people of this region.
Strain-tunable van der Waals interactions in few-layer black phosphorus
Authors: Huang Shenyang , Zhang Guowei , Fan Fengren , Song Chaoyu , Wang Fanjie , Xing Qiaoxia , Wang Chong , Wu Hua , Yan Hugen 
Published: June 2019

AbstractInterlayer interactions in 2D materials, also known as van der Waals (vdWs) interactions, play a critical role in the physical properties of layered materials. It is fascinating to manipulate the vdWs interaction, and hence to “redefine” the material properties. Here, we demonstrate that in-plane biaxial strain can effectively tune the vdWs interaction of few-layer black phosphorus with thickness of 2-10 layers, using infrared spectroscopy. Surprisingly, our results reveal that in-plane tensile strain efficiently weakens the interlayer coupling, even though the sample shrinks in the vertical direction due to the Poisson effect, in sharp contrast to one’s intuition. Moreover, density functional theory (DFT) calculations further confirm our observations and indicate a dominant role of the puckered lattice structure. Our study highlights the important role played by vdWs interactions in 2D materials during external physical perturbations.
Tri-phasic engineered wetland system for effective treatment of azo dye-based wastewater
Authors: Kumar Yeruva Dileep , Ranadheer Palle , Kiran Kumar A. , Venkata Mohan S. 
Published: June 2019

AbstractSynthetic dyes are a major class of compounds used in the textile industry. The effluents released from these industries are carcinogenic and mutagenic in nature and pose potential threat to all life forms. Here, we designed a novel tri-phasic engineered flow through wetland system (TEWS) with three specific microenvironments (tri-phasic aquatic systems) integrated in a defined sequence to effectively treat azo dye-based textile wastewater. Tank 1 with free-floating and emergent aquatic macrophytes create anaerobic/anoxic microenvironments and facilitate reduction of the azo dye molecules. Tank 2 consists of submerged macrophytes which create an aerophilic microenvironment that direct the oxidation of carbon compounds along with nitrification. Tank 3 with free-floating plants and aquatic animals acts as filter feeder intended to remove suspended solids and colour, as well as residual carbon. The strategically designed TEWS attributes to 76%/87% of dye/chemical oxygen demand removal. The morphological toxicity test of plants and fishes illustrate the nontoxic nature of treated effluents. Thus, TEWS not only decolorizes the azo dye but also removes its toxic and mutagenic components.
Geographical origin traceability of Cabernet Sauvignon wines based on Infrared fingerprint technology combined with chemometrics
Authors: Hu Xiao-Zhen , Liu Si-Qi , Li Xiao-Hong , Wang Chuan-Xian , Ni Xin-Lu , Liu Xia , Wang Yang , Liu Yuan , Xu Chang-Hua 
Published: June 2019

AbstractMid-infrared (MIR) and near-infrared (NIR) spectroscopy combined with chemometrics were explored to classify Cabernet Sauvignon wines from different countries (Australia, Chile and China). Commercial wines (n = 540) were scanned in transmission mode using MIR and NIR, and their characteristic fingerprint bands were extracted at 1750-1000 cm−1 and 4555-4353 cm−1. Through the identification system of Tri-step infrared spectroscopy, the correlation between macroscopic chemical fingerprints and geographical regions was explored more deeply. Furthermore, Principal component analysis (PCA), soft independent modelling of class analogy (SIMCA) and discriminant analysis (DA) based on MIR and NIR spectra were used to visualize or discriminate differences between samples and to realize geographical origin traceability of Cabernet Sauvignon wines. Through “external test set (n = 157)” validation, SIMCA models correctly classified 97%, 97% and 92% of Australian, Chilean and Chinese Cabernet Sauvignon wines, while the DA models correctly classified 86%, 85% and 77%, respectively. Based on unique digital fingerprints of spectroscopy (FT-MIR and FT-NIR) associated with chemometrics, geographical origin traceability was achieved in a more comprehensive, effective and rapid manner. The developed database models based on IR fingerprint spectroscopy with chemometrics could provide scientific basis and reference for geographical origin traceability of Cabernet Sauvignon wines (Australia, Chile and China).
A comparative characterization of the circulating miRNome in whole blood and serum of HCC patients
Authors: Pascut Devis , Krmac Helena , Gilardi Francesca , Patti Riccardo , Calligaris Raffaella , Crocè Lory Saveria , Tiribelli Claudio 
Published: June 2019

AbstractmiRNAs are considered promising non-invasive biomarkers. Serum represents the major source of biomarkers, being readily accessible for many analytical tests. Recently, whole blood has drawn increasing interest in biomarker studies due to the presence of cancer-interacting cells and circulating cancer cells. Although Hepatocellular Carcinoma (HCC) is the seventh most frequent cancer worldwide, fragmented information exists regarding the miRNome characterization in blood and serum. We profiled the circulatory miRNome of paired serum and blood samples from 20 HCC patients, identifying 274 miRNA expressed in serum and 670 in blood, most of them still uncharacterized. 157 miRNA significantly differ between the two biofluids with 28 exclusively expressed in serum. Six miRNA clusters significantly characterize the two compartments, with the cluster containing miR-4484, miR-1281, miR-3178, miR-3613-3p, miR-4532, miR-4668-5p, miR-1825, miR-4487, miR-455-3p, miR-940 having the highest average expression in serum compared to blood. The ontological analysis revealed a role of these miRNAs in cancer progression, vascular invasion and cancer immune surveillance thought the regulation of DUSP1, PD-L1 and MUC1. Taken together, these results provide the most comprehensive contribution to date towards a complete miRNome profile of blood and serum for HCC patients. We show a consistent portion of circulatory miRNAs being still unknown.
Znf703 is a novel RA target in the neural plate border
Authors: Janesick Amanda , Tang Weiyi , Ampig Kristen , Blumberg Bruce 
Published: June 2019

AbstractZnf703 is an RAR- and Wnt-inducible transcription factor that exhibits a complex expression pattern in the developing embryo: Znf703 mRNA is found in the early circumblastoporal ring, then later throughout the neural plate and its border, and subsequently in the mid/hindbrain and somites. We show that Znf703 has a different and separable function in early mesoderm versus neural crest and placode development. Independent of its early knockdown phenotype on Gdf3 and Wnt8, Znf703 disrupts patterning of distinct neural crest migratory streams normally delineated by Sox10, Twist, and Foxd3 and inhibits otocyst formation and otic expression of Sox10 and Eya1. Furthermore, Znf703 promotes massive overgrowth of SOX2+ cells, disrupting the SoxB1 balance at the neural plate border. Despite prominent expression in other neural plate border-derived cranial and sensory domains, Znf703 is selectively absent from the otocyst, suggesting that Znf703 must be specifically cleared or down-regulated for proper otic development. We show that mutation of the putative Groucho-repression domain does not ameliorate Znf703 effects on mesoderm, neural crest, and placodes. We instead provide evidence that Znf703 requires the Buttonhead domain for transcriptional repression.
The relationship between the structural characteristics of lactobacilli-EPS and its ability to induce apoptosis in colon cancer cells in vitro
Authors: Tukenmez Ummugulsum , Aktas Busra , Aslim Belma , Yavuz Serkan 
Published: June 2019

AbstractColon cancer is one of the most common cancer around the world. Exopolysaccharides (EPSs) produced by lactobacilli as potential prebiotics have been found to have an anti-tumor effect. In this study, lyophilized EPSs of four Lactobacillus spp. for their impact on apoptosis in colon cancer cells (HT-29) was evaluated using flow cytometry. The relationship between capability of a lactobacilli-EPS to induce apoptosis and their monosaccharide composition, molecular weight (MW), and linkage type was investigated by HPLC, SEC, and NMR, respectively. Changes in apoptotic-markers were examined by qPCR and Western Blotting. EPSs were capable of inhibiting proliferation in a time-dependent manner and induced apoptosis via increasing the expression of Bax, Caspase 3 and 9 while decreasing Bcl-2 and Survivin. All EPSs contained mannose, glucose, and N-acetylglucosamine with different relative proportions. Some contained arabinose or fructose. MW ranged from 102–104Da with two or three fractions. EPS of L. delbrueckii ssp. bulgaricus B3 having the highest amount of mannose and the lowest amount of glucose, showed the highest apoptosis induction. In conclusion, lactobacilli-EPSs inhibit cell proliferation in HT-29 via apoptosis. Results suggest that a relationship exists between the ability of EPS to induce apoptosis and its mannose and glucose composition.