bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age Takes Hold of Us by Surprise: Conceptualizing Vulnerabilities in Aging as the Timing of Adverse Events
Authors: Vanhoutte Bram 
Published: July 2019

Abstract
Objectives: Aging in the public eye can be distilled to a limited number of adverse events, such as loss of health, partnership and wealth. While these events are a constitutive part of “normal aging,” they do not occur uniformly at the same time point in the life course. This study investigates to what extent bereavement, functional health loss, and onset of poverty are adequate markers of aging, and illustrates inequalities in their timing according to cohort, gender, class, education, and ethnicity.
Methods: The English Longitudinal Study of Ageing (ELSA), collected over seven waves (2002–2016) (n = 7,890) is examined in an event history framework. Cox proportional hazard models are used with the Andersen Gill extension in case of multiple failures per respondent.
Results: Persistent associations of lower occupational class, lower education, and having a black or minority ethnic background are found with increased hazards of functional health loss and wealth loss. Earlier born cohorts have lower hazards for functional health loss, wealth loss, and bereavement. Women have higher hazards for bereavement, and lower hazards for wealth loss.
Discussion: The timing of adverse events is a crucial gateway through which existing social inequalities are transferred into unequal aging pathways.
“… Above All, It’s a Matter of  This Person’s Quality of Life”: Health Care Professionals’ Perspectives on Deprescribing in Older Patients With Limited Life Expectancy
Authors: Lundby Carina , Graabæk Trine , Ryg Jesper , Søndergaard Jens , Pottegård Anton , Nielsen Dorthe Susanne 
Published: July 2019

Abstract

Background and Objectives
Deprescribing may be particularly relevant in older people with limited life expectancy. In order to effectively carry out deprescribing in this population, it is important to understand the perspectives of the full spectrum of health care professionals (HCPs) involved in the management of these patients’ medication. Thus, we aimed to explore different HCPs’ perspectives on deprescribing in older patients with limited life expectancy.

Research Design and Methods
Six qualitative focus group interviews were conducted using a semistructured approach. The groups comprised HCPs from both primary and secondary care, including family physicians (FPs), geriatricians, clinical pharmacologists, clinical pharmacists, nurses, and health care assistants. Interviews were audio recorded and transcribed verbatim. Results were analyzed using systematic text condensation.

Results
A total of 32 HCPs participated in the study (median age of 40.5 years; 22% male). The analysis elicited three main themes related to HCPs’ perspectives on deprescribing in older patients with limited life expectancy: (a) Approaching deprescribing, (b) Taking responsibility, and (c) Collaboration across professions. Within themes, subthemes were identified and analyzed.

Discussion and Implications
Our results imply that different groups of HCPs consider deprescribing an essential aspect of providing good care for older people with limited life expectancy and find that all HCPs play a crucial role in the deprescribing process, with FPs having the primary responsibility. In order to facilitate deprescribing among this population, however, the collaboration between different HCPs should be improved.
A physically based model for the electrical conductivity of water-saturated porous media
Authors: Duy Thanh Luong , Jougnot Damien , Van Do Phan , Van Nghia A Nguyen 
Published: July 2019

SUMMARY
Electrical conductivity is one of the most commonly used geophysical method for reservoir and environmental studies. Its main interest lies in its sensitivity to key properties of storage and transport in porous media. Its quantitative use therefore depends on the efficiency of the petrophysical relationship to link them. In this work, we develop a new physically based model for estimating electrical conductivity of saturated porous media. The model is derived assuming that the porous media is represented by a bundle of tortuous capillary tubes with a fractal pore-size distribution. The model is expressed in terms of the porosity, electrical conductivity of the pore liquid and the microstructural parameters of porous media. It takes into account the interface properties between minerals and pore water by introducing a surface conductivity. Expressions for the formation factor and hydraulic tortuosity are also obtained from the model derivation. The model is then successfully compared with published data and performs better than previous models. The proposed approach also permits to relate the electrical conductivity to other transport properties such as the hydraulic conductivity.
Removing mantle lithosphere under orogens: delamination versus convective thinning
Authors: Lei Tian , Li Zhong-Hai , Liu Mian 
Published: July 2019

SUMMARY
Thinning of the lithosphere under continental collisional orogens is often attributed to delamination or convective thinning. Both processes remove part or all of the mantle lithosphere that has become denser and gravitationally unstable. Previous studies mostly focused on the different thermomagmatic consequences of these two processes; the dynamic links between them, and the critical conditions for one or the other process to dominate lithosphere thinning, remain uncertain. Here, we used high-resolution thermomechanical models with various rheology (linear viscous, power-law viscous and/or the extended Drucker–Prager plasticity) to systematically investigate the dynamics of delamination and convective thinning under collisional orogens. Our results show that convective thinning is favoured in models of linear (Newtonian) viscous rheology and low viscosity $({10^{19}}\!-\! {10^{20}}\,\,{\rm{Pa}} \, {\rm{s}})$. Power-law viscous rheology promotes strain localization, which reduces the effective viscosity and may lead to localized rising of the asthenosphere to the crustal base, thus triggering delamination. Further strain localization and stronger delamination are predicted with inclusion of plastic rheology in the model. These results indicate that convective thinning and delamination are dynamically linked and can occur in the same orogeny. Their relative dominance during orogenesis may be distinguished by the resulting spatiotemporal evolutions of thermal perturbation, magmatism and elevation changes. We applied the models to show that the evolution of the Central Anatolian Plateau is consistent with the dominance of convective thinning, whereas delamination played a major role in thinning the mantle lithosphere under central-northern Tibetan Plateau.
Upper-mantle density structure in the Philippine Sea and adjacent region and its relation to tectonics
Authors: Liang Qing , Chen Chao , Kaban Mikhail K , Thomas Maik 
Published: July 2019

SUMMARY
The evolution of the Philippine Sea Plate (PSP) since Jurassic is one of the key issues in the dynamics of lithosphere and mantle. The related studies benefited mostly from seismic tomography which provides velocity structures in the upper mantle. However, the upper-mantle structure is not well resolved compared to the continental areas due to the lack of seismic data in the Philippine Sea. We employ a 3-D gravity inversion constrained by an initial model based on the S-wave tomography (SL2013sv; Schaeffer & Lebedev 2013) to image the density structure of the upper mantle of the PSP and adjacent region. The resulting model shows a three-layer pattern of vertical high-low-high density variation in the upper mantle under the PSP. The thin high-density layer evidences for strong oceanic lithosphere in the West Philippine Sea. The relatively low dense mantle located below the PSP possibly originates from the asthenosphere. The PSP differs from the Pacific and the Indian-Australian plates in the whole depth range, while its structure is similar to the eastern Eurasian and Sunda plates. In the depth range, 200–300 km, the relative high-density zone beneath PSP extends to the Sunda Plate and to the eastern Eurasian Plate. We further estimated the conversion factor of our density model and the velocity model (SL2013sv; Schaeffer & Lebedev 2013) in order to locate the changes of compositional effects in the upper mantle. The negative conversion factor indicates that the compositional changes primarily affect the density anomalies beneath the PSP. We, therefore, describe the layered density structures as ‘sandwich’ pattern, which is unique and different from adjacent regions.
Semirational design and engineering of grapevine glucosyltransferases for enhanced activity and modified product selectivity
Authors: Joshi Rakesh , Trinkl Johanna , Haugeneder Annika , Härtl Katja , Franz-Oberdorf Katrin , Giri Ashok , Hoffmann Thomas , Schwab Wilfried 
Published: July 2019

AbstractUridine diphosphate-dependent glycosyltransferases (UGTs) catalyze the transfer of a diversity of sugars to several acceptor molecules and often exhibit distinct substrate specificity. Modulation of glycosyltransferases for increased catalytic activity and altered substrate or product specificity are the key manipulations for the biotechnological use of glycosyltransferases in various biosynthetic processes. Here, we have engineered the binding pocket of three previously characterized Vitis vinifera glycosyltransferases, UGT88F12, UGT72B27 and UGT92G6, by structure-guided in silico mutagenesis to facilitate the interactions of active site residues with flavonol glucosides and thus modify substrate specificity and activity. Site-directed mutagenesis at selected sites, followed with liquid chromatography–mass spectrometry based activity assays, exhibited that mutant UGTs were altered in product selectivity and activity as compared to the wild-type enzymes. Mutant UGTs produced larger amounts of flavonol di-monosaccharide glucosides, which imply that the mutations led to structural changes that increased the volume of the binding pocket to accommodate a larger substrate and to release larger products at ease. Mutants showed increased activity and modified product specificity. Thus, structure-based systematic mutations of the amino acid residues in the binding pocket can be explored for the generation of engineered UGTs for diverse biotechnological applications.
Financial protection and equity of access to health services with the free maternal and child health initiative in Lao PDR
Authors: Nagpal Somil , Masaki Emiko , Pambudi Eko Setyo , Jacobs Bart 
Published: July 2019

Abstract
Though Lao People’s Democratic Republic (Lao PDR) has made considerable progress in improving maternal and child health (MCH), significant disparities exist nationwide, with the poor and geographically isolated ethnic groups having limited access to services. In its pursuit of universal health coverage, the government introduced a Free MCH initiative in 2011, which has recently been subsumed within the new National Health Insurance (NHI) programme. Although this was a major national health financing reform, there have been few evaluations of the extent to which it improved equitable access to MCH services. We analyse surveys that provide information on demand-side and supply-side factors influencing access and utilization of free MCH services, especially for vulnerable groups. This includes two rounds of household surveys (2010 and 2013) in southern Lao PDR involving, respectively 2766 and 2911 women who delivered within 24 months prior to each survey. These data have been analysed according to the socio-economic status, geographic location and ethnicity of women using the MCH services as well as any associated out-of-pocket expenses and structural quality of these services. Two other surveys analysed here focused on human resources for health and structural quality of health facilities. Together, these data point to persistent large inequities in access and financial protection that need to be addressed. Significant differences were found in the utilization of health services by both economic status and ethnicity. Relatively large costs for institutional births were incurred by the poor and did not decline between 2010 and 2013 whereby there was no significant impact on financial protection. The overall benefit incidence of the universal programme was not pro-poor. The inequity was accentuated by issues related to distribution and nature of human resources, supply-side readiness and thus quality of care provided across different geographical areas.
How to do (or not to do) … using the standardized patient method to measure clinical quality of care in LMIC health facilities
Authors: King Jessica J C , Das Jishnu , Kwan Ada , Daniels Benjamin , Powell-Jackson Timothy , Makungu Christina , Goodman Catherine 
Published: July 2019

Abstract
Standardized patients (SPs), i.e. mystery shoppers for healthcare providers, are increasingly used as a tool to measure quality of clinical care, particularly in low- and middle-income countries where medical record abstraction is unlikely to be feasible. The SP method allows care to be observed without the provider’s knowledge, removing concerns about the Hawthorne effect, and means that providers can be directly compared against each other. However, their undercover nature means that there are methodological and ethical challenges beyond those found in normal fieldwork. We draw on a systematic review and our own experience of implementing such studies to discuss six key steps in designing and executing SP studies in healthcare facilities, which are more complex than those in retail settings. Researchers must carefully choose the symptoms or conditions the SPs will present in order to minimize potential harm to fieldworkers, reduce the risk of detection and ensure that there is a meaningful measure of clinical care. They must carefully define the types of outcomes to be documented, develop the study scripts and questionnaires, and adopt an appropriate sampling strategy. Particular attention is required to ethical considerations and to assessing detection by providers. Such studies require thorough planning, piloting and training, and a dedicated and engaged field team. With sufficient effort, SP studies can provide uniquely rich data, giving insights into how care is provided which is of great value to both researchers and policymakers.
Client perspectives on an outreach approach for HIV prevention targeting Indonesian MSM and transwomen
Authors: Nugroho Adi , Erasmus Vicki , Krier Sarah E , Reviagana Kusnindyah P , Laksmono Pipiet A , Widihastuti Asti , Richardus Jan Hendrik 
Published: July 2019

Abstract
This study explored clients’ perspective on an outreach approach to promote HIV testing in Indonesia targeting men who have sex with men (MSM) and transgender women (transwomen or waria). Semi-structured qualitative interviews were conducted with 32 individuals (21 MSM and 11 waria) who had received services from outreach workers (OWs) in five cities in Indonesia. Participants in this study reported positive experiences with the outreach approach and perceived OWs as their motivators in accessing HIV testing as well as HIV care and treatment. OWs provided easy-to-understand HIV information. Clients expected OWs to be well-trained and more creative in performing outreach. They perceived that the Internet and social media have helped them considerably to stay in touch with OWs. Yet, they expressed that such virtual contacts could not simply replace the face-to-face contact, especially for waria. Furthermore, clients suggested outreach to be delivered in a more appealing manner, for example through activities that may facilitate clients learning professional or life skills. They also asserted that as an HIV prevention approach, outreach needs to use more positive framing and go beyond HIV and health contents, chiefly for the youth. Future outreach programmes should facilitate OWs in providing tailored services based on the level and type of support that the clients need, and in applying varied proportion and levels of sophistication in the use of online and virtual platforms for outreach.
Synapse diversity and synaptome architecture in human genetic disorders
Authors: Grant Seth G N 
Published: July 2019

Abstract
Over 130 brain diseases are caused by mutations that disrupt genes encoding the proteome of excitatory synapses. These include neurological and psychiatric disorders with early and late onset such as autism, schizophrenia and depression and many other rarer conditions. The proteome of synapses is highly complex with over 1000 conserved proteins which are differentially expressed generating a vast, potentially unlimited, number of synapse types. The diversity of synapses and their location in the brain are described by the synaptome. A recent study has mapped the synaptome across the mouse brain, revealing that synapse diversity is distributed into an anatomical architecture observed at scales from individual dendrites to the whole systems level. The synaptome architecture is built from the hierarchical expression and assembly of proteins into complexes and supercomplexes which are distributed into different synapses. Mutations in synapse proteins change the synaptome architecture leading to behavioral phenotypes. Mutations in the mechanisms regulating the hierarchical assembly of the synaptome, including transcription and proteostasis, may also change synapse diversity and synaptome architecture. The logic of synaptome hierarchical assembly provides a mechanistic framework that explains how diverse genetic disorders can converge on synapses in different brain circuits to produce behavioral phenotypes.
Pax7, Pax3 and Mamstr genes are involved in skeletal muscle impaired regeneration of dy2J/dy2J mouse model of Lama2-CMD
Authors: Yanay Nurit , Elbaz Moran , Konikov-Rozenman Jenya , Elgavish Sharona , Nevo Yuval , Fellig Yakov , Rabie Malcolm , Mitrani-Rosenbaum Stella , Nevo Yoram 
Published: July 2019

AbstractCongenital muscular dystrophy type-1A (Lama2-CMD) and Duchenne muscular dystrophy (DMD) result from deficiencies of laminin-α2 and dystrophin proteins, respectively. Although both proteins strengthen the sarcolemma, they are implicated in clinically distinct phenotypes. We used RNA-deep sequencing (RNA-Seq) of dy2J/dy2J, Lama2-CMD mouse model, skeletal muscle at 8 weeks of age to elucidate disease pathophysiology. This study is the first report of dy2J/dy2J model whole transcriptome profile. RNA-Seq of the mdx mouse model of DMD and wild-type (WT) mouse was carried as well in order to enable a novel comparison of dy2J/dy2J to mdx. A large group of shared differentially expressed genes (DEGs) was found in dy2J/dy2J and mdx models (1834 common DEGs, false discovery rate [FDR] < 0.05). Enrichment pathway analysis using ingenuity pathway analysis showed enrichment of inflammation, fibrosis, cellular movement, migration and proliferation of cells, apoptosis and necrosis in both mouse models (P-values 3E-10–9E-37). Via canonical pathway analysis, actin cytoskeleton, integrin, integrin-linked kinase, NF-kB, renin–angiotensin, epithelial–mesenchymal transition, and calcium signaling were also enriched and upregulated in both models (FDR < 0.05). Interestingly, significant downregulation of Pax7 was detected in dy2J/dy2J compared to upregulation of this key regeneration gene in mdx mice. Pax3 and Mamstr genes were also downregulated in dy2J/dy2J compared to WT mice. These results may explain the distinct disease course and severity in these models. While the mdx model at that stage shows massive regeneration, the dy2J/dy2J shows progressive dystrophic process. Our data deepen our understanding of the molecular pathophysiology and suggest new targets for additional therapies to upregulate regeneration in Lama2-CMD.
CFAP70 mutations lead to male infertility due to severe astheno-teratozoospermia. A case report
Authors: Beurois Julie , Martinez Guillaume , Cazin Caroline , Kherraf Zine-Eddine , Amiri-Yekta Amir , Thierry-Mieg Nicolas , Bidart Marie , Petre Graciane , Satre Véronique , Brouillet Sophie , Touré Aminata , Arnoult Christophe , Ray Pierre F , Coutton Charles 
Published: July 2019

Abstract
The use of high-throughput sequencing techniques has allowed the identification of numerous mutations in genes responsible for severe astheno-teratozoospermia due to multiple morphological abnormalities of the sperm flagella (MMAF). However, more than half of the analysed cases remain unresolved suggesting that many yet uncharacterised gene defects account for this phenotype. Based on whole-exome sequencing data from a large cohort of 167 MMAF-affected subjects, we identified two unrelated affected individuals carrying a homozygous deleterious mutation in CFAP70, a gene not previously linked to the MMAF phenotype. One patient had a homozygous splice variant c.1723-1G>T, altering a consensus splice acceptor site of CFAP70 exon 16, and one had a likely deleterious missense variant in exon 3 (p.Phe60Ile). The CFAP70 gene encodes a regulator protein of the outer dynein arms (ODA) strongly expressed in the human testis. In the sperm cells from the patient carrying the splice variant, immunofluorescence (IF) experiments confirmed the absence of the protein in the sperm flagellum. Moreover, IF analysis showed the absence of markers for the ODAs and the central pair complex of the axoneme. Interestingly, whereas CFAP70 staining was present in sperm cells from patients with mutations in the three other MMAF-related genes ARMC2, FSIP2 and CFAP43, we observed an absence of staining in sperm cells from patients mutated in the WDR66 gene, suggesting a possible interaction between two different axonemal components. In conclusion, this work provides the first evidence that loss of CFAP70 function causes MMAF and that ODA-related proteins may be crucial for the assembly and/or stability of the flagellum axoneme in addition to its motility.
Deep neural networks for analysis of fisheries surveillance video and automated monitoring of fish discards
Authors: French Geoff , Mackiewicz Michal , Fisher Mark , Holah Helen , Kilburn Rachel , Campbell Neil , Needle Coby 
Published: July 2019

Abstract
We report on the development of a computer vision system that analyses video from CCTV systems installed on fishing trawlers for the purpose of monitoring and quantifying discarded fish catch. Our system is designed to operate in spite of the challenging computer vision problem posed by conditions on-board fishing trawlers. We describe the approaches developed for isolating and segmenting individual fish and for species classification. We present an analysis of the variability of manual species identification performed by expert human observers and contrast the performance of our species classifier against this benchmark. We also quantify the effect of the domain gap on the performance of modern deep neural network-based computer vision systems.