bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between nocturnal polyuria and non‐dipping blood pressure in male patients with lower urinary tract symptoms
Authors: Takayama Misato , Omori So , Iwasaki Kazuhiro , Shiomi Ei , Takata Ryo , Sugimura Jun , Abe Takaya , Obara Wataru 
Published: May 2018

ObjectiveThe aim of the present study was to examine factors of nocturnal polyuria and blood pressure variability in male patients with lower urinary tract symptoms (LUTS) who were treated.MethodsTwo hundred and forty‐two male patients with LUTS who were treated recorded frequency volume charts. We investigated their urinary condition and characteristics, medical history, and medications. Thirty‐four of these patients underwent ambulatory blood pressure monitoring (ABPM) for 24 hours to evaluate blood pressure variability.ResultsIn the present study, 194 patients (80.2%) had nocturia and 136 (56.2%) had nocturnal polyuria (NP). Among patients with nocturia (≥2 voids/night), 130 (67.0%) had nocturnal polyuria, and 26 of those with nocturia (13.4%) had reduced functional bladder capacity. The use of 2 or more antihypertensive medications was significantly higher in the NP than non‐NP group (22.8% vs. 12.3%; P = .035). Significantly more patients in the NP group had non‐dipping blood pressure (P = .037). Non‐dipping blood pressure was considered a potential factor for NP.ConclusionWe suggest that treatment of non‐dipping blood pressure may improve NP.
Bacterial microbiota assemblage in Aedes albopictus mosquitoes and its impacts on larval development
Authors: Wang Xiaoming , Liu Tong , Wu Yang , Zhong Daibin , Zhou Guofa , Su Xinghua , Xu Jiabao , Sotero Charity F. , Sadruddin Adnan A. , Wu Kun , Chen Xiao‐Guang , Yan Guiyun 
Published: May 2018

AbstractInteractions between bacterial microbiota and mosquitoes play an important role in mosquitoes’ capacity to transmit pathogens. However, microbiota assemblages within mosquitoes and the impact of microbiota in environments on mosquito development and survival remain unclear. This study examined microbiota assemblages and the effects of aquatic environment microbiota on the larval development of the Aedes albopictus mosquito, an important dengue virus vector. Life table studies have found that reducing bacterial load in natural aquatic habitats through water filtering and treatment with antibiotics significantly reduced the larva‐to‐adult emergence rate. This finding was consistent in two types of larval habitats examined—discarded tires and flowerpots, suggesting that bacteria play a crucial role in larval development. Pyrosequencing of the bacterial 16S rRNA gene was used to determine the diversity of bacterial communities in larval habitats and the resulting numbers of mosquitoes under both laboratory and field conditions. The microbiota profiling identified common shared bacteria among samples from different years; further studies are needed to determine whether these bacteria represent a core microbiota. The highest microbiota diversity was found in aquatic habitats, followed by mosquito larvae, and the lowest in adult mosquitoes. Mosquito larvae ingested their bacterial microbiota and nutrients from aquatic habitats of high microbiota diversity. Taken together, the results support the observation that Ae. albopictus larvae are able to utilize diverse bacteria from aquatic habitats and that live bacteria from aquatic habitats play an important role in larval mosquito development and survival. These findings provide new insights into bacteria's role in mosquito larval ecology.
Aging attenuates adenosine triphosphate‐induced, but not muscarinic and nicotinic, cutaneous vasodilation in men
Authors: Fujii Naoto , Nishiyasu Takeshi , Sigal Ronald J. , Boulay Pierre , McGarr Gregory W. , Kenny Glen P. 
Published: May 2018

AbstractObjectiveWe evaluated the hypothesis that aging attenuates muscarinic, nicotinic, and ATP‐related cutaneous vasodilation.MethodsIn 11 young (24 ± 4 years) and 11 older males (61 ± 8 years), CVC was assessed at 3 forearm skin sites that were infused with either: (i) methacholine (muscarinic receptor agonist, 5 doses: 0.0125, 0.25, 5, 100, 2000 mmol/L), (ii) nicotine (nicotinic receptor agonist, 5 doses: 1.2, 3.6, 11, 33, 100 mmol/L), or (iii) ATP (purinergic receptor agonist, 5 doses: 0.03, 0.3, 3, 30, 300 mmol/L). Each agonist was administered for 25 minutes per dose.ResultsWe showed that CVC at all doses of methacholine did not differ between groups. Similarly, no between‐group differences in CVC were observed during nicotine administration at all doses administered. By contrast, while no differences in CVC were measured during the administration of ATP at low (0.03 and 0.3 mmol/L) or high (300 mmol/L) concentrations, CVC was reduced in the older relative to the young males at moderate concentrations of ATP (3 mmol/L: 23 ± 6 vs 40 ± 13%max, 30 mmol/L: 62 ± 11 vs 83 ± 8%max, both P ≤ .05).ConclusionsWe show that aging attenuates ATP‐induced, but not muscarinic or nicotinic, cutaneous vasodilation in men.
Attention‐deficit/hyperactivity disorder symptoms and suicidal behavior in adult psychiatric outpatients
Authors: Stickley Andrew , Tachimori Hisateru , Inoue Yosuke , Shinkai Takahiro , Yoshimura Reiji , Nakamura Jun , Morita Gihei , Nishii Shigeki , Tokutsu Yuki , Otsuka Yuka , Egashira Kazuteru , Inoue Miyuki , Kubo Takamitsu , Tesen Hirofumi , Takashima Naoyuki , Tominaga Hirotaka , Koyanagi Ai , Kamio Yoko 
Published: May 2018

AimWe aimed to examine the association between attention‐deficit/hyperactivity disorder (ADHD) symptoms and suicidal behavior in psychiatric outpatients and whether this association differs among patients with different psychiatric disorders.MethodsCross‐sectional data came from the Japan Prevalence Study of Adult ADHD at Psychiatric Outpatient Care, which included psychiatric outpatients aged 18–65 years recruited from one university hospital and three general psychiatric outpatient clinics in Kitakyushu City, Fukuoka, Japan from April 2014 to January 2015 (N = 864). The Adult ADHD Self‐Report Scale (ASRS) Screener was used to collect information on ADHD symptoms. Reports of current and lifetime suicidal behavior were also obtained. A multivariable Poisson regression analysis was used to examine the association between ADHD symptoms and suicidal behavior.ResultsAfter adjusting for covariates there was a strong association between possible ADHD (ASRS ≥14) and suicidal behavior with prevalence ratios ranging from 1.17 (lifetime suicidal ideation) to 1.59 (lifetime suicide attempt) and 2.36 (current suicidal ideation). When ASRS strata were used, there was a dose–response association between increasing ADHD symptoms and suicidal ideation and suicide attempts. Analyses of individual ICD‐10 psychiatric disorders showed that associations varied across disorders and that for anxiety disorder, ADHD symptoms were significantly linked to all forms of suicidal behavior.ConclusionADHD symptom severity is associated with an increased risk for suicidal behavior in general psychiatric outpatients. As ADHD symptoms are common among adult psychiatric outpatients, detecting and treating ADHD in this population may be important for preventing suicidal behavior.
Prevalence rate of Internet addiction among Japanese college students: Two cross‐sectional studies and reconsideration of cut‐off points of Young's Internet Addiction Test in Japan
Authors: Tateno Masaru , Teo Alan R. , Shiraishi Masaki , Tayama Masaya , Kawanishi Chiaki , Kato Takahiro A. 
Published: May 2018

AimDue to variation in estimates of the prevalence of Internet addiction (IA) in prior research, we conducted two cross‐sectional studies over 2 years investigating the prevalence rate of IA in college students in Japan and reconsidered appropriate cut‐off points of a self‐rating scale to screen possible IA.MethodsThis study was composed of two parts: survey I in 2014 and survey II in 2016, which were conducted in the same schools with an interval of 2 years. The study questionnaire included questions about demographics and Internet use, and Young's Internet Addiction Test (IAT). Additionally, the subjects in survey II were asked about self‐reported IA.ResultsThere were 1005 respondents in total with a mean age (± SD) of 18.9 ± 1.3 years. The mean IAT scores remained stable between 2014 and 2016: 45.2 ± 12.6 in survey I and 45.5 ± 13.1 in survey II (overall mean IAT score of 45.4 ± 13.0). With respect to self‐reported IA in survey II, a total of 21.6% admitted to having IA (score of 5 or 6 on a 6‐point Likert scale). We categorized these subjects as IA, and the remainder as non‐IA. The mean IAT score showed a significant difference between these two groups (57.8 ± 14.3 vs 42.1 ± 10.7, P < 0.001).ConclusionThe severity of IA symptoms among Japanese college students has appeared stable in recent years, with mean IAT scores of over 40. Our results suggest that a screening score cut‐off of 40 on the IAT could be reconsidered and that 50 might be proposed for the cut‐off.
Evaluation of DEFB1 polymorphisms in individuals with chronic periodontitis and diabetes mellitus type 2 in a population of northeastern Brazil
Authors: Soraia Aguiar de Melo Dias Rayanne , Carlos Mourão Pinho Roberto , Almeida Felipe Rodrigues de , Alfredo Farias Bandeira Francisco , Celerino da Silva Ronaldo , Crovella Sergio , de Carvalho Farias Vajgel Bruna , Cimões Renata 
Published: May 2018

AbstractAimsThe role of genetic variations in genes related to innate response, as β‐defensin‐1 (DEFB1), in the context of chronic periodontitis (CP) and diabetes mellitus type 2 (DM2), is still not clear. The present study evaluates the distribution of DEFB1 single nucleotide polymorphisms (SNPs) 5′‐untranslated (5′UTR) region and its relation with the CP in DM2 individuals in northeastern Brazilians.MethodsTwo hundred and eighty individuals participated in the study, being 116 DM2+CP, 95 CP, and 69 healthy individuals. Three known DEFB1 functional SNPs [‐52 G > A (rs1799946), ‐44 C > G (rs1800972), ‐20 G > A (rs11362)] were genotyped with allele‐specific assays.ResultsAssociation was found for the DEFB1 ‐20 G > A SNP. The G allele, the GA and GG genotypes were significantly (P < 0.05) more frequent in the DM2+CP (59.5%, 50%, and 34.5%, respectively) and CP (61%, 44.2%, and 38.9%, respectively) than in healthy individuals (26.8%, 36.2%, and 8.7%, respectively). The GCG and ACG combinations (‐52, ‐44, ‐20) were significantly more frequent among DM2+CP and CP than in the healthy individuals.ConclusionThe results indicate that genetic variations of DEFB1 gene (SNP‐20: G allele and GA and GG genotypes) and the DEFB1 5′UTR haplotypes (GCG and ACG) may be associated with a susceptibility to CP in DM2 individuals as well as CP individuals without DM2.
Measuring Women's Covert Use of Modern Contraception in Cross‐Sectional Surveys
Authors: Choiriyyah Ifta , Becker Stan 
Published: May 2018

AbstractA proportion of women in couples use contraception without their partners’ knowledge. There are two principal ways to measure this covert use in cross‐sectional surveys like the Demographic and Health Surveys (DHS). First is a direct question, “Does your husband/partner know that you are using a method of family planning?” Second is an indirect method: the reports of both partners to the question on contraceptive use are matched, and if the woman reports a modern contraceptive method and the male partner reports nonuse, her use is considered covert. For 21 DHS surveys for which both estimates could be made, there are large discrepancies between the two. We found that a proxy variable—responses to the question, “Would you say that using contraception is mainly your decision, mainly your husband's/partner's decision, or did you both decide together?”—has high sensitivity and specificity for classifying those in the open category for both methods and those in the covert category for both methods. Recommendations are that the direct question be reinstated in the DHS and that the indirect method not be used by itself but in conjunction with the decision‐making variable.
The erythrocyte alloimmunisation in patients with sickle cell anaemia: a systematic review
Authors: da Cunha Gomes E. G. , Machado L. A. F. , de Oliveira L. C. , Neto J. F. N. 
Published: May 2018

SUMMARYTransfusion therapy is a common practice in the treatment of anaemia and can cause erythrocyte alloimmunisation. To systematise data related to erythrocyte alloimmunisation in patients with sickle cell disease (SCD), a bibliographic search was carried out in September 2017 to search for studies in four electronic databases. (i) Referring to the original work, (ii) being cohort or case–control, (iii) having been developed with individuals with SCD and (iv) having evaluated the erythrocyte alloimmunisation. Two reviewers identified the articles for inclusion in the study, extracted the predetermined data and carried out the evaluation of the methodological quality of the work. 21 studies were selected; the studies included data on 20 636 individuals (children and adults), were mostly published in the last 10 years, were developed in the United States and had high methodological quality. The occurrence of erythrocyte alloimmunisation ranged from 4·4 to 76%, and there was a higher rate of alloimmunisation against antigens of the Rh system. The risk factors for alloimmunisation were age; gender (female); red blood cell (RBC) units received; presence of ≥1 autoantibodies, TNF‐α, interleukin (IL1B), human leukocyte antigens (HLA)‐DRB1 gene polymorphisms; first blood transfusion (BT) after 5 years of age, transfusion episodic, multiple or during inflammatory events, acute chest syndrome (ACS) and vase‐occlusive crisis (VOC); increased percentage of CD41 T memory cells; and positive direct antiglobulin test. Transfusion policies should be developed to protect the patient and his or her health based on the main factors associated with its incidence.
In vitro bactericidal activity of 465–470 nm blue light phototherapy and aminolevulinic acid on Staphylococcus pseudintermedius
Authors: Bae Seulgi , Oh Taeho 
Published: May 2018

BackgroundStaphylococcus pseudintermedius is the principal pathogen causing bacterial skin infections in dogs. Photodynamic therapy (PDT) involving the combination of light and a topical photosensitizer is used to treat human skin infections. Although the antimicrobial effects of PDT have been demonstrated using in vivo and in vitro studies in humans, its effects on dogs and their pathogens are unclear.ObjectivesThe aim of this study was to demonstrate the in vitro efficacy of PDT over a 465–470 nm spectrum to kill S. pseudintermedius using δ‐aminolevulinic acid (ALA) as the photosensitizer.MethodsSix S. pseudintermedius isolates from canine skin were exposed to blue light‐emitting diodes (LEDs) at 465–470 nm, with or without ALA. The light doses were 18.4, 36.8 and 55.2 J/cm2. The number of colony‐forming units and optical densities of broth cultures were measured and then compared with Dunnett's test. Bacterial viability was monitored using fluorescence microscopy and the fluorescence intensity values were compared with a paired Student's t‐test.ResultsBlue light inhibited the growth of S. pseudintermedius; the effect significantly increased with the addition of ALA as a photosensitizer and with increasing light doses. Live/dead staining confirmed that PDT reduced bacterial viability and exerted an antibacterial effect.Conclusion and clinical importanceBlue light has a strong antibacterial effect on S. pseudintermedius in a light dose‐dependent manner. ALA alone did not exhibit bactericidal action, but its combination with blue light increased the effect of PDT compared to blue light alone.
Complete Genomic Sequence of Pseudoalteromonas sp. Strain SAO4-4, a Protease-Producing Bacterium Isolated from Seawater of the Atlantic Ocean
Authors: Tang Bai-Lu , Rong Jin-Cheng , Dang Yan-Ru , Xie Bin-Bin , Chen Xiu-Lan , Zhang Xi-Ying 
Published: May 2018

ABSTRACT

The complete genome of
Pseudoalteromonas
sp. strain SAO4-4, a protease-producing bacterium from seawater, is composed of two circular chromosomes and one plasmid. This genome sequence will provide a better understanding of the ecological roles of protease-producing bacteria in the degradation of organic matter in marine aquatic environments.
Complete Genome Sequence of a Novel Recombinant
Citrus Tristeza Virus
, a Resistance-Breaking Isolate from Uruguay
Authors: Benítez-Galeano María José , Vallet Thomas , Carrau Lucía , Hernández-Rodríguez Lester , Bertalmío Ana , Rivas Fernando , Rubio Leticia , Maeso Diego , Vignuzzi Marco , Moratorio Gonzalo , Colina Rodney 
Published: May 2018

ABSTRACT

We report here the complete genome sequence of a
Citrus tristeza virus
(CTV) from Uruguay, sequenced by using Illumina and Sanger sequencing technology. This CTV DSST-17 genome clustered within genotype resistance breaking (RB) and presents two recombination events.
Draft Genome Sequence of the Novel Enterobacter cloacae Strain amazonensis, a Highly Heavy Metal-Resistant Bacterium from a Contaminated Stream in Amazonas, Brazil
Authors: Astolfi Maria Clara Tavares , Carvalho Elen Bethleen de Souza , de Barros Adriane Menezes , Pinto Marcelo Valente , de Lacerda Luna Barrôco , Nogueira Viviane Brito , Lopes Eraldo Ferreira , Astolfi-Filho Spartaco 
Published: May 2018

ABSTRACT

Here, we report the draft genome of the
Enterobacter cloacae
strain amazonensis, a bacterium highly resistant to mercury that was isolated from a metal- and sewage-contaminated stream in Amazonas, Brazil. The exploration of the 5.0-Mb genome revealed 104 genes encoding resistance to toxic compounds and heavy metals, highlighting the potential biotechnological applications of this strain.
Complete Genome Sequences of Three Tomato Aspermy Virus Isolates in Japan
Authors: Inoue Sota , Tamura Misato , Ugaki Masashi , Suzuki Masashi 
Published: May 2018

ABSTRACTWe report here the first complete nucleotide sequences of genomic RNAs of threeTomato aspermy virus(TAV) isolates in Japan. Analysis of these sequences showed that they have unique characteristics in RNAs 2 and 3. The Japanese isolates are similar to each other compared to other TAVs.
Complete Genome Sequence of Multidrug-Resistant Edwardsiella ictaluri Strain MS-17-156
Authors: Abdelhamed Hossam , Tekedar Hasan C. , Ozdemir Ozan , Hsu Chuan-Yu , Arick Mark A. , Karsi Attila , Lawrence Mark L. 
Published: May 2018

ABSTRACT

Edwardsiella ictaluri
is a significant pathogen of cultured fish, particularly channel catfish. Here, we present the complete genome sequence of a multidrug-resistant
E. ictaluri
strain, MS-17-156, isolated from diseased channel catfish. The genome sequence of this multidrug-resistant strain is expected to help us understand the molecular mechanism of antibiotic resistance in this important pathogen.
Preseason intrinsic risk factors—associated odds estimate the exposure to proximal lower limb injury throughout the season among professional football players
Authors: Liporaci Rogério Ferreira , Saad Marcelo Camargo , Bevilaqua-Grossi Débora , Riberto Marcelo 
Published: May 2018

Background/aimFootball players frequently face the occurrence of non-contact musculoskeletal injuries. The purpose of the study was to verify whether the evaluation of combined risk factors could produce a score system to determine the probability of injury in football players during the Brazilian football season.MethodsSixty-two male professional soccer players recruited from the first and second division soccer teams, with ages between 18 and 36 years. Functional performance evaluations were carried out at the beginning of the preseason which included strength and jumping tests, history of injuries and characteristics of athletes.Results and conclusionsThe results were grouped and a score/monogram was constructed.
Expansion of IL-23 receptor bearing TNFR2+ T cells is associated with molecular resistance to anti-TNF therapy in Crohn’s disease
Authors: Schmitt Heike , Billmeier Ulrike , Dieterich Walburga , Rath Timo , Sonnewald Sophia , Reid Stephen , Hirschmann Simon , Hildner Kai , Waldner Maximilian J , Mudter Jonas , Hartmann Arndt , Grützmann Robert , Neufert Clemens , Münster Tino , Neurath Markus F , Atreya Raja 
Published: May 2018

ObjectiveAnti-tumour necrosis factor (TNF) antibodies are successfully used for treatment of Crohn’s disease. Nevertheless, approximately 40% of patients display failure to anti-TNF therapy. Here, we characterised molecular mechanisms that are associated with endoscopic resistance to anti-TNF therapy.DesignMucosal and blood cells were isolated from patients with Crohn’s disease prior and during anti-TNF therapy. Cytokine profiles, cell surface markers, signalling proteins and cell apoptosis were assessed by microarray, immunohistochemistry, qPCR, ELISA, whole organ cultures and FACS.ResultsResponders to anti-TNF therapy displayed a significantly higher expression of TNF receptor 2 (TNFR2) but not IL23R on T cells than non-responders prior to anti-TNF therapy. During anti-TNF therapy, there was a significant upregulation of mucosal IL-23p19, IL23R and IL-17A in anti-TNF non-responders but not in responders. Apoptosis-resistant TNFR2+IL23R+ T cells were significantly expanded in anti-TNF non-responders compared with responders, expressed the gut tropic integrins α4β7, and exhibited increased expression of IFN-γ, T-bet, IL-17A and RORγt compared with TNFR2+IL23R− cells, indicating a mixed Th1/Th17-like phenotype. Intestinal TNFR2+IL23R+ T cells were activated by IL-23 derived from CD14+ macrophages, which were significantly more present in non-responders prior to anti-TNF treatment. Administration of IL-23 to anti-TNF-treated mucosal organ cultures led to the expansion of CD4+IL23R+TNFR2+ lymphocytes. Functional studies demonstrated that anti-TNF-induced apoptosis in mucosal T cells is abrogated by IL-23.ConclusionsExpansion of apoptosis-resistant intestinal TNFR2+IL23R+ T cells is associated with resistance to anti-TNF therapy in Crohn’s disease. These findings identify IL-23 as a suitable molecular target in patients with Crohn’s disease refractory to anti-TNF therapy.
Transient Invariant and Quasi-Invariant Structures in an Example of an Aperiodically Time Dependent Fluid Flow
Authors: Fortunati Alessandro , Wiggins Stephen 
Published: May 2018

Starting from the concept of invariant KAM tori for nearly-integrable Hamiltonian systems with periodic or quasi-periodic nonautonomous perturbation, the paper analyzes the “analogue” of this class of invariant objects when the dependence on time is aperiodic. The investigation is carried out in a model motivated by the problem of a traveling wave in a channel over a smooth, quasi- and asymptotically flat (from which the “transient” feature) bathymetry, representing a case in which the described structures play the role of barriers to fluid transport in phase space. The paper provides computational evidence for the existence of transient structures also for “large” values of the perturbation size, as a complement to the rigorous results already proven by the first author for real-analytic bathymetry functions.
Bifurcation Diagram of a Map with Multiple Critical Points
Authors: Romera M. , Pastor G. , Danca M.-F. , Martin A. , Orue A. B. , Montoya F. , Encinas L. Hernández , Ţundrea E. 
Published: May 2018

In this work a conjecture to draw the bifurcation diagram of a map with multiple critical points is enunciated. The conjecture is checked by using two quartic maps in order to verify that the bifurcation diagrams obtained according to the conjecture contain all the periodic orbits previously counted by Xie and Hao for maps with four laps. We show that a map with split bifurcation contains more periodic orbits than those counted by these authors for a map with the same number of laps.