bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protective effect of hydroxysafflor yellow A against acute kidney injury via the TLR4/NF-κB signaling pathway
Authors: Bai Juan , Zhao Jinyi , Cui Dongxiao , Wang Fan , Song Ying , Cheng Lianghua , Gao Kai , Wang Jin , Li Long , Li Shujun , Jia Yanyan , Wen Aidong 
Published: June 2018

AbstractThis study aimed to evaluate the protective effect of hydroxysafflor yellow A (HSYA) on ischemia/reperfusion (I/R)-induced acute kidney injury via the TLR4/NF-κB pathway, both in vitro and in vivo. Rats were subjected to removal of the right kidney and I/R injury to the left kidney. Rats subjected to renal I/R injury were treated with HSYA at 0.5 h prior to I/R injury. Renal function, histopathological analysis, and cells apoptosis were measured in vivo. In vitro, proximal renal tubular cells (HK-2) were subjected to hypoxia/reoxygenation (H/R). Apoptotic cell death and inflammatory cytokines, Toll-like receptor 4 (TLR4), and nuclear factor (NF)-κB expression were determined. Treatment of I/R rats with HSYA markedly reduced the levels of serum creatinine and blood urea nitrogen, attenuated renal cell apoptosis, alleviated changes in renal tissue morphology, and reduced IL-1β, TNF-α, and caspase-3 release. In vitro, HSYA effectively decreased NF-κB p65 and inflammatory cytokines, such as IL-1β, TNF-α, and IL-6. Thus, HSYA can protect renal function from I/R injury by ameliorating acute kidney injury and partly by promoting tubular cell survival via the TLR4/NF-κB pathway. These results suggest that HSYA can be used to prevent I/R-induced acute kidney injury.
Ductile bulk metallic glass by controlling structural heterogeneities
Authors: Scudino S. , Bian J. J. , Shakur Shahabi H. , Şopu D. , Sort J. , Eckert J. , Liu G. 
Published: June 2018

AbstractA prerequisite to utilize the full potential of structural heterogeneities for improving the room-temperature plastic deformation of bulk metallic glasses (BMGs) is to understand their interaction with the mechanism of shear band formation and propagation. This task requires the ability to artificially create heterogeneous microstructures with controlled morphology and orientation. Here, we analyze the effect of the designed heterogeneities generated by imprinting on the tensile mechanical behavior of the Zr52.5Ti5Cu18Ni14.5Al10 BMG by using experimental and computational methods. The imprinted material is elastically heterogeneous and displays anisotropic mechanical properties: strength and ductility increase with increasing the loading angle between imprints and tensile direction. This behavior occurs through shear band branching and their progressive rotation. Molecular dynamics and finite element simulations indicate that shear band branching and rotation originates at the interface between the heterogeneities, where the characteristic atomistic mechanism responsible for shear banding in a homogeneous glass is perturbed.
Colorectal cancer-associated Streptococcus infantarius subsp. infantarius differ from a major dairy lineage providing evidence for pathogenic, pathobiont and food-grade lineages
Authors: Kaindi Dasel Wambua Mulwa , Kogi-Makau Wambui , Lule Godfrey Nsereko , Kreikemeyer Bernd , Renault Pierre , Bonfoh Bassirou , Otaru Nize , Schmid Thomas , Meile Leo , Hattendorf Jan , Jans Christoph 
Published: June 2018

AbstractStreptococcus infantarius subsp. infantarius (Sii), a member of the Streptococcus bovis/Streptococcus equinus complex (SBSEC), predominates as dairy-adapted and non-adapted variants in fermented dairy products (FDP) in East and West Africa. Epidemiologic data suggest an association with colorectal cancer for most SBSEC members, including Sii from Kenyan patients. Phylogenetic relationships of East African human (EAH) isolates to those of dairy and pathogenic origin were analysed to better estimate potential health implications via FDP consumption. The MLST-derived population structure was also evaluated to provide host, disease, geography and dairy adaptation associations for 157 SBSEC isolates, including 83 novel Sii/SBSEC isolates of which 40 originated from Kenyan colonoscopy patients. Clonal complex (CC) 90 was delineated as potential pathogenic CC for Sii. Single EAH, West African dairy (WAD), food and animal Sii isolates clustered within CC-90, suggesting a potential link to pathogenic traits for CC-90. The majority of EAH and WAD Sii were clustered in a shared clade distinct from CC-90 and East African dairy (EAD) isolates. This indicates shared ancestry for the EAH and WAD clade and limitations to translate disease associations of EAH and CC-90 to EAD Sii, which could support the separation of pathogenic, pathobiont/commensal and food lineages.
All-depth dispersion cancellation in spectral domain optical coherence tomography using numerical intensity correlations
Authors: Jensen Mikkel , Israelsen Niels Møller , Maria Michael , Feuchter Thomas , Podoleanu Adrian , Bang Ole 
Published: June 2018

AbstractIn ultra-high resolution (UHR-) optical coherence tomography (OCT) group velocity dispersion (GVD) must be corrected for in order to approach the theoretical resolution limit. One approach promises not only compensation, but complete annihilation of even order dispersion effects, and that at all sample depths. This approach has hitherto been demonstrated with an experimentally demanding ‘balanced detection’ configuration based on using two detectors. We demonstrate intensity correlation (IC) OCT using a conventional spectral domain (SD) UHR-OCT system with a single detector. IC-SD-OCT configurations exhibit cross term ghost images and a reduced axial range, half of that of conventional SD-OCT. We demonstrate that both shortcomings can be removed by applying a generic artefact reduction algorithm and using analytic interferograms. We show the superiority of IC-SD-OCT compared to conventional SD-OCT by showing how IC-SD-OCT is able to image spatial structures behind a strongly dispersive silicon wafer. Finally, we question the resolution enhancement of $$\sqrt{2}$$

2

that IC-SD-OCT is often believed to have compared to SD-OCT. We show that this is simply the effect of squaring the reflectivity profile as a natural result of processing the product of two intensity spectra instead of a single spectrum.
Large-Area, Cost-Effective, Ultra-Broadband Perfect Absorber Utilizing Manganese in Metal-Insulator-Metal Structure
Authors: Aalizadeh Majid , Khavasi Amin , Butun Bayram , Ozbay Ekmel 
Published: June 2018

AbstractAchieving broadband absorption has been a topic of intensive research over the last decade. However, the costly and time consuming stage of lithography has always been a barrier for the large-area and mass production of absorbers. In this work, we designed, fabricated, and characterized a lithography-free, large-area compatible, omni-directional, ultra-broadband absorber that consists of the simplest geometrical configuration for absorbers: Metal-Insulator-Metal (MIM). We introduced and utilized Manganese (Mn) for the first time as a very promising metal for broadband absorption applications. We optimized the structure step-by-step and compared Mn against the other best candidates introduced so far in broadband absorption structures and showed the better performance of Mn compared to them. It also has the advantage of being cheaper compared to metals like gold that has been utilized in many patterned broadband absorbers. We also presented the circuit model of the structure. We experimentally achieved over 94 percent average absorption in the range of 400–900 nm (visible and above) and we obtained absorption as high as 99.6 percent at the wavelength of 626.4 nm. We also experimentally demonstrated that this structure retains broadband absorption for large angles up to 70 degrees.
Early-life and pubertal stress differentially modulate grey matter development in human adolescents
Authors: Tyborowska Anna , Volman Inge , Niermann Hannah C. M. , Pouwels J. Loes , Smeekens Sanny , Cillessen Antonius H. N. , Toni Ivan , Roelofs Karin 
Published: June 2018

AbstractAnimal and human studies have shown that both early-life traumatic events and ongoing stress episodes affect neurodevelopment, however, it remains unclear whether and how they modulate normative adolescent neuro-maturational trajectories. We characterized effects of early-life (age 0–5) and ongoing stressors (age 14–17) on longitudinal changes (age 14 to17) in grey matter volume (GMV) of healthy adolescents (n = 37). Timing and stressor type were related to differential GMV changes. More personal early-life stressful events were associated with larger developmental reductions in GMV over anterior prefrontal cortex, amygdala and other subcortical regions; whereas ongoing stress from the adolescents’ social environment was related to smaller reductions over the orbitofrontal and anterior cingulate cortex. These findings suggest that early-life stress accelerates pubertal development, whereas an adverse adolescent social environment disturbs brain maturation with potential mental health implications: delayed anterior cingulate maturation was associated with more antisocial traits – a juvenile precursor of psychopathy.
Extracellular nanovesicles released from the commensal yeast Malassezia sympodialis are enriched in allergens and interact with cells in human skin
Authors: Johansson Henrik J. , Vallhov Helen , Holm Tina , Gehrmann Ulf , Andersson Anna , Johansson Catharina , Blom Hans , Carroni Marta , Lehtiö Janne , Scheynius Annika 
Published: June 2018

AbstractMalassezia sympodialis is a dominant commensal fungi in the human skin mycobiome but is also associated with common skin disorders including atopic eczema (AE). M. sympodialis releases extracellular vesicles, designated MalaEx, which are carriers of small RNAs and allergens, and they can induce inflammatory cytokine responses. Here we explored how MalaEx are involved in host-microbe interactions by comparing protein content of MalaEx with that of the parental yeast cells, and by investigating interactions of MalaEx with cells in the skin. Cryo-electron tomography revealed a heterogeneous population of MalaEx. iTRAQ based quantitative proteomics identified in total 2439 proteins in all replicates of which 110 were enriched in MalaEx compared to the yeast cells. Among the MalaEx enriched proteins were two of the M. sympodialis allergens, Mala s 1 and s 7. Functional experiments indicated an active binding and internalization of MalaEx into human keratinocytes and monocytes, and MalaEx were found in close proximity of the nuclei using super-resolution fluorescence 3D-SIM imaging. Our results provides new insights into host-microbe interactions, supporting that MalaEx may have a role in the sensitization and maintenance of inflammation in AE by containing enriched amounts of allergens and with their ability to interact with skin cells.
Functional switching of ascorbate peroxidase 2 of rice (OsAPX2) between peroxidase and molecular chaperone
Authors: Hong Sung Hyun , Tripathi Bhumi Nath , Chung Moon-Soo , Cho Chuloh , Lee Sungbeom , Kim Jin-Hong , Bai Hyoung-Woo , Bae Hyeun-Jong , Cho Jae-Young , Chung Byung Yeoup , Lee Seung Sik 
Published: June 2018

AbstractAscorbate peroxidase (APX) is a class I haem-containing peroxidase, which catalyses the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. APX plays a central role in the elimination of intracellular reactive oxygen species (ROS) and protects plants from the oxidative damage that can occur as a result of biotic and abiotic stresses. At present, the only known function of APX is as a peroxidase. However, in this study, we demonstrate that Oryza sativa APX2 also operates as a molecular chaperone in rice. The different functions of OsAPX2 correlate strongly with its structural conformation. The high-molecular-weight (HMW) complexes had chaperone activity, whereas the low-molecular-weight (LMW) forms displayed predominantly APX activity. The APX activity was effectively inhibited by sodium azide, which is an inhibitor of haem-containing enzymes, but this did not affect the protein’s activity as a chaperone. Additionally, the OsAPX2 conformational changes could be regulated by salt and heat stresses and these stimulated OsAPX2 dissociation and association, respectively. Our results provide new insight into the roles of APXs.
Social status and prenatal testosterone exposure assessed via second-to-fourth digit ratio affect 6–9-year-old children’s prosocial choices
Authors: Horn Lisa , Hungerländer Niklas A. , Windhager Sonja , Bugnyar Thomas , Massen Jorg J. M. 
Published: June 2018

AbstractProsocial behaviour (i.e., voluntary behaviour intended to benefit another) seems to be fully developed in children by the age of 6 years. However, questions about which factors modify prosocial behaviour at that age remain understudied. Here we used a resource allocation paradigm to test prosocial behaviour in 6–9-year-old school children. They could decide between a “selfish” (i.e., one sticker for themselves) and a “prosocial” option (i.e., one sticker for themselves and one for the receiver) and we tested whether friendship, social status and prenatal androgen exposure (approximated by the 2nd to 4th digit ratio; 2D:4D) influenced children’s prosocial choices. We found that children behaved prosocially, and that their prosocial tendencies were negatively correlated with prenatal androgen exposure; i.e., children with high 2D:4D ratios (reflecting low prenatal androgen exposure) acted more prosocially than children with low 2D:4D ratios. Further, their social status in the classroom influenced their choices: children with fewer interaction partners chose the “prosocial” option more often than more ‘popular’ children. However, they did so irrespectively of whether they were paired with a recipient or not. Our results highlight the importance of considering social, as well as physiological factors when investigating prosocial behaviour in children.
Structural Neural Correlates of Physiological Mirror Activity During Isometric Contractions of Non-Dominant Hand Muscles
Authors: Maudrich Tom , Kenville Rouven , Lepsien Jöran , Villringer Arno , Ragert Patrick 
Published: June 2018

AbstractMirror Activity (MA) describes involuntarily occurring muscular activity in contralateral homologous limbs during unilateral movements. This phenomenon has not only been reported in patients with neurological disorders (i.e. Mirror Movements) but has also been observed in healthy adults referred to as physiological Mirror Activity (pMA). However, despite recent hypotheses, the underlying neural mechanisms and structural correlates of pMA still remain insufficiently described. We investigated the structural correlates of pMA during isometric contractions of hand muscles with increasing force demands on a whole-brain level by means of voxel-based morphometry (VBM) and tract-based spatial statistics (TBSS). We found significant negative correlations between individual tendencies to display pMA and grey matter volume (GMV) in the right anterior cingulate cortex (ACC) as well as fractional anisotropy (FA) of white matter (WM) tracts of left precuneus (PrC) during left (non-dominant) hand contractions. No significant structural associations for contractions of the right hand were found. Here we extend previously reported functional associations between ACC/PrC and the inhibtion of intrinsically favoured mirror-symmetrical movement tendencies to an underlying structural level. We provide novel evidence that the individual structural state of higher order motor/executive areas upstream of primary/secondary motor areas might contribute to the phenomen of pMA.
Focus-Induced Photoresponse: a novel way to measure distances with photodetectors
Authors: Pekkola Oili , Lungenschmied Christoph , Fejes Peter , Handreck Anke , Hermes Wilfried , Irle Stephan , Lennartz Christian , Schildknecht Christian , Schillen Peter , Schindler Patrick , Send Robert , Valouch Sebastian , Thiel Erwin , Bruder Ingmar 
Published: June 2018

AbstractWe present the Focus-Induced Photoresponse (FIP) technique, a novel approach to optical distance measurement. It takes advantage of a universally-observed phenomenon in photodetector devices, an irradiance-dependent responsivity. This means that the output from a sensor is not only dependent on the total flux of incident photons, but also on the size of the area in which they fall. If probe light from an object is cast on the detector through a lens, the sensor response depends on how far in or out of focus the object is. We call this the FIP effect. Here we demonstrate how to use the FIP effect to measure the distance to that object. We show that the FIP technique works with different sensor types and materials, as well as visible and near infrared light. The FIP technique operates on a working principle, which is fundamentally different from all established distance measurement methods and hence offers a way to overcome some of their limitations. FIP enables fast optical distance measurements with a simple single-pixel detector layout and minimal computational power. It allows for measurements that are robust to ambient light even outside the wavelength range accessible with silicon.
In planta proximity dependent biotin identification (BioID)
Authors: Khan Madiha , Youn Ji-Young , Gingras Anne-Claude , Subramaniam Rajagopal , Desveaux Darrell 
Published: June 2018

AbstractProximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.
Genome-wide transcriptional analysis of submerged lotus reveals cooperative regulation and gene responses
Authors: Wang Bei , Jin Qijiang , Zhang Xiao , Mattson Neil S. , Ren Huihui , Cao Jing , Wang Yanjie , Yao Dongrui , Xu Yingchun 
Published: June 2018

AbstractFlooding severely limits plant growth even for some aquatic plants. Although much work has been done on submergence response of some important crop plants, little is known about the response mechanism of aquatic plants, i.e. lotus (Nelumbo nucifera). In this study, we investigated the genome-wide regulation lotus genes in response to submergence stress by high-throughput mRNA sequencing. A total of 4002 differentially expressed genes (DEGs) in lotus upon submergence stress. Among them, 1976 genes were up-regulated and 2026 down-regulated. Functional annotation of these genes by Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis revealed that they were mainly involved in processes of oxidation-reduction, abiotic stimuli, cellular metabolism and small molecule metabolism. Based on these data, previous work and quantitative RT-PCR (RT-qPCR) validation, we constructed a cooperative regulation network involved in several important DEGs in regards to the antioxidant system, disease resistance, hypoxia resistance and morphological adaptation. Further work confirmed that several innate immunity genes were induced during submergence and might confer higher resistance to lotus rot disease. In conclusion, these results provide useful information on molecular mechanisms underlying lotus responses to submergence stress.
Unexplored photoluminescence from bulk and mechanically exfoliated few layers of Bi2Te3
Authors: Gupta Bipin Kumar , Sultana Rabia , Singh Satbir , Singh Vijeta , Awana Geet , Gupta Anurag , Singh Bahadur , Srivastava A. K. , Srivastava O. N. , Auluck S. , Awana V. P. S. 
Published: June 2018

AbstractWe report the exotic photoluminescence (PL) behaviour of 3D topological insulator Bi2Te3 single crystals grown by customized self-flux method and mechanically exfoliated few layers (18 ± 2 nm)/thin flakes obtained by standard scotch tape method from as grown Bi2Te3 crystals. The experimental PL studies on bulk single crystal and mechanically exfoliated few layers of Bi2Te3 evidenced a broad red emission in the visible region from 600–690 nm upon 375 nm excitation wavelength corresponding to optical band gap of 2 eV. These findings are in good agreement with our theoretical results obtained using the ab initio density functional theory framework. Interestingly, the observed optical band gap is several times larger than the known electronic band gap of ~0.15 eV. The experimentally observed 2 eV optical band gap in the visible region for bulk as well as for mechanically exfoliated few layers Bi2Te3 single crystals clearly rules out the quantum confinement effects in the investigated samples which are well known in the 2D systems like MoS2,WS2, WSe2, and MoSe2 for 1–3 layers.
Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of Leptospira at the Onset of Disease
Authors: Esteves Lisa M. , Bulhões Sara M. , Branco Claudia C. , Carreira Teresa , Vieira Maria L. , Gomes-Solecki Maria , Mota-Vieira Luisa 
Published: June 2018

AbstractCurrently, direct detection of Leptospira can be done in clinical laboratories by conventional and by real-time PCR (qRT-PCR). We tested a biobank of paired samples of serum and urine from the same patient (202 patients) presenting at the hospital in an area endemic for leptospirosis using qRT-PCR followed by high resolution melting (HRM) analysis. The results were compared with those obtained by conventional nested PCR and with the serologic gold standard microscopic agglutination test (MAT). Differences were resolved by sequencing. qRT-PCR-HRM was positive for 46 of the 202 patients (22.7%, accuracy 100%) which is consistent with known prevalence of leptospirosis in the Azores. MAT results were positive for 3 of the 46 patients (6.5%). Analysis of paired samples allowed us to identify the illness point at which patients presented at the hospital: onset, dissemination or excretion. The melting curve analysis of Leptospira species revealed that 60.9% (28/46) of patients were infected with L. interrogans and 39.1% (18/46) were infected with L. borgpetersenii, both endemic to the Azores. We validated the use of qRT-PCR-HRM for diagnosis of leptospirosis and for identification of the Leptospira species at the earliest onset of infection in a clinical setting, in less than 2 hours.
RNA-Seq reveals large quantitative differences between the transcriptomes of outbreak and non-outbreak locusts
Authors: Bakkali M. , Martín-Blázquez R. 
Published: June 2018

AbstractOutbreaks of locust populations repeatedly devastate economies and ecosystems in large parts of the world. The consequent behavioural shift from solitarious to gregarious and the concomitant changes in the locusts’ biology are of relevant scientific interest. Yet, research on the main locust species has not benefitted from recent advances in genomics. In this first RNA-Seq study on Schistocerca gregaria, we report two transcriptomes, including many novel genes, as well as differential gene expression results. In line with the large biological differences between solitarious and gregarious locusts, almost half of the transcripts are differentially expressed between their central nervous systems. Most of these transcripts are over-expressed in the gregarious locusts, suggesting positive correlations between the levels of activity at the population, individual, tissue and gene expression levels. We group these differentially expressed transcripts by gene function and highlight those that are most likely to be associated with locusts’ phase change either in a species-specific or general manner. Finally, we discuss our findings in the context of population-level and physiological events leading to gregariousness.