bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring Parkinson’s disease symptoms during daily life: a feasibility study
Authors: Heijmans Margot , Habets Jeroen G. V. , Herff Christian , Aarts Jos , Stevens An , Kuijf Mark L. , Kubben Pieter L. 
Published: September 2019

AbstractParkinson’s disease symptoms are most often charted using the MDS-UPDRS. Limitations of this approach include the subjective character of the assessments and a discrepant performance in the clinic compared to the home situation. Continuous monitoring using wearable devices is believed to eventually replace this golden standard, but measurements often lack a parallel ground truth or are only tested in lab settings. To overcome these limitations, this study explores the feasibility of a newly developed Parkinson’s disease monitoring system, which aims to measure Parkinson’s disease symptoms during daily life by combining wearable sensors with an experience sampling method application. Twenty patients with idiopathic Parkinson’s disease participated in this study. During a period of two consecutive weeks, participants had to wear three wearable sensors and had to complete questionnaires at seven semi-random moments per day on their mobile phone. Wearable sensors collected objective movement data, and the questionnaires containing questions about amongst others Parkinson’s disease symptoms served as parallel ground truth. Results showed that participants wore the wearable sensors during 94% of the instructed timeframe and even beyond. Furthermore, questionnaire completion rates were high (79,1%) and participants evaluated the monitoring system positively. A preliminary analysis showed that sensor data could reliably predict subjectively reported OFF moments. These results show that our Parkinson’s disease monitoring system is a feasible method to use in a diverse Parkinson’s disease population for at least a period of two weeks. For longer use, the monitoring system may be too intense and wearing comfort needs to be optimized.
The sequence and de novo assembly of Takifugu bimaculatus genome using PacBio and Hi-C technologies
Authors: Zhou Zhixiong , Liu Bo , Chen Baohua , Shi Yue , Pu Fei , Bai Huaqiang , Li Leibin , Xu Peng 
Published: September 2019

AbstractTakifugu bimaculatus is a native teleost species of the southeast coast of China where it has been cultivated as an important edible fish in the last decade. Genetic breeding programs, which have been recently initiated for improving the aquaculture performance of T. bimaculatus, urgently require a high-quality reference genome to facilitate genome selection and related genetic studies. To address this need, we produced a chromosome-level reference genome of T. bimaculatus using the PacBio single molecule sequencing technique (SMRT) and High-through chromosome conformation capture (Hi-C) technologies. The genome was assembled into 2,193 contigs with a total length of 404.21 Mb and a contig N50 length of 1.31 Mb. After chromosome-level scaffolding, 22 chromosomes with a total length of 371.68 Mb were constructed. Moreover, a total of 21,117 protein-coding genes and 3,471 ncRNAs were annotated in the reference genome. The highly accurate, chromosome-level reference genome of T. bimaculatus provides an essential genome resource for not only the genome-scale selective breeding of T. bimaculatus but also the exploration of the evolutionary basis of the speciation and local adaptation of the Takifugu genus.
Targeted genomic CRISPR-Cas9 screen identifies MAP4K4 as essential for glioblastoma invasion
Authors: Prolo Laura M. , Li Amy , Owen Scott F. , Parker Jonathon J. , Foshay Kara , Nitta Ryan T. , Morgens David W. , Bolin Sara , Wilson Christy M. , Vega L Johana C. M. , Luo Emily J. , Nwagbo Gigi , Waziri Allen , Li Gordon , Reimer Richard J. , Bassik Michael C. , Grant Gerald A. 
Published: September 2019

AbstractAmong high-grade brain tumors, glioblastoma is particularly difficult to treat, in part due to its highly infiltrative nature which contributes to the malignant phenotype and high mortality in patients. In order to better understand the signaling pathways underlying glioblastoma invasion, we performed the first large-scale CRISPR-Cas9 loss of function screen specifically designed to identify genes that facilitate cell invasion. We tested 4,574 genes predicted to be involved in trafficking and motility. Using a transwell invasion assay, we discovered 33 genes essential for invasion. Of the 11 genes we selected for secondary testing using a wound healing assay, 6 demonstrated a significant decrease in migration. The strongest regulator of invasion was mitogen-activated protein kinase 4 (MAP4K4). Targeting of MAP4K4 with single guide RNAs or a MAP4K4 inhibitor reduced migration and invasion in vitro. This effect was consistent across three additional patient derived glioblastoma cell lines. Analysis of epithelial-mesenchymal transition markers in U138 cells with lack or inhibition of MAP4K4 demonstrated protein expression consistent with a non-invasive state. Importantly, MAP4K4 inhibition limited migration in a subset of human glioma organotypic slice cultures. Our results identify MAP4K4 as a novel potential therapeutic target to limit glioblastoma invasion.
Apatinib Mesylate in the treatment of advanced progressed lung adenocarcinoma patients with EGFR-TKI resistance —A Multicenter Randomized Trial
Authors: Fang Ping , Zhang Liqin , Zhang Xianru , Yu Jiawen , Sun Jun , Jiang Qi-an , Zha Mingbao , Nesterova Anastasia P. , Cao Hongbao 
Published: September 2019

AbstractFew pieces of evidence have been published on the use of Apatinib Mesylate (AM) against EGFR-TKI resistance in lung adenocarcinoma (LA) patients. Here, we investigate the clinical efficacy and safety of AM in the treatment of advanced progressed epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI) resistant LA patients. We conducted a double-blind, randomized controlled trial in 68 patients admitted to 18 hospitals of Anhui province in China. The efficacy and safety of AM treatment were evaluated in terms of progression-free survival (PFS), objective response rate (ORR), and disease control rate (DCR), as well as related adverse events (AE). A literature knowledge database analysis and a pathway model reconstruction were performed to decipher the relevant mechanism may be involved. Our results showed that, compared to the control group, AM presented improved efficacy in PFS (P = 0.033), ORR (P < 0.001), and DCR (P < 0.001). No significant difference was observed between case and control group in terms of AE, and no drug-related death occurred. Pathway analysis supports that Apatinib can be repurposed for the treatment of LA. Our results suggested that AM could be a potential option for advanced progressed LA patients to combat EGFR-TKI resistance.
Eugenol Reduces LDL Cholesterol and Hepatic Steatosis in Hypercholesterolemic Rats by Modulating TRPV1 Receptor
Authors: Harb Amani A. , Bustanji Yasser K. , Almasri Ihab M. , Abdalla Shtaywy S. 
Published: September 2019

AbstractEugenol, a component of essential oils of medicinal and food plants, has a hypolipidemic effect in experimental animals although its mechanism of action is still unclear. This study aims to explore the mechanism of the hypolipidemic effect of eugenol in rats fed a high cholesterol and fat diet (HCFD). Eugenol significantly reduced total cholesterol (TC), low-density lipoproteins (LDL), atherogenic index (AI) but not high-density lipoproteins (HDL) or triglycerides (TG). Eugenol also decreased steatosis and hepatic inflammation in liver sections, decreased hepatomegaly, and the hepatic marker enzymes alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activity and increased the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) activity in hypercholesterolemic rats. Eugenol did not inhibit hepatic 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase but caused down-regulation of transient receptor potential vanilloid (TRPV1) channels in the liver. Docking simulation using fast, rigid exhaustive docking (FRED) software indicated a tail-up/head-down interaction of eugenol with TRPV1 channel. Data indicate that eugenol does not inhibit HMG-CoA reductase but rather induces its action by interaction with TRPV1 channels.
Environmental versus operational drivers of drifting FAD beaching in the Western and Central Pacific Ocean
Authors: Escalle Lauriane , Scutt Phillips Joe , Brownjohn Maurice , Brouwer Stephen , Sen Gupta Alex , Van Sebille Erik , Hampton John , Pilling Graham 
Published: September 2019

AbstractIn an effort to increase purse seine fishing efficiency for tropical tunas, over 30,000 drifting Fish Aggregating Devices (dFADs) are deployed every year by fishers in the Western and Central Pacific Ocean (WCPO). The use of dFADs also impacts ecosystems, in particular through marine pollution and dFAD beaching. This paper presents the first estimate of dFAD beaching events in the WCPO (>1300 in 2016–2017) and their distribution. Lagrangian simulations of virtual dFADs, released subject to contrasting deployment distributions, help us determine the relative importance of operational versus environmental drivers of dFADs drifting to beaching areas. The highest levels of beaching, occurring on Papua New Guinea and Solomon Islands, are likely a result of the prevailing westward oceanic circulation and subsequent local processes driving dFADs towards land. Similarly, high beaching rates in Tuvalu appear to be due to the general circulation of the WCPO. In contrast, beaching in Kiribati Gilbert Islands appear to be more strongly related to dFAD deployment strategy. These findings indicate that reducing beaching events via changes in deployment locations may be difficult. As such, management approaches combining dFAD deployment limits, the use of biodegradable dFADs, recoveries at-sea close to sensitive areas and/or beached dFAD removal should be considered.
Stem cells from the dental apical papilla in extracellular matrix hydrogels mitigate inflammation of microglial cells
Authors: Tatic Natalija , Rose Felicity R. A. J. , des Rieux Anne , White Lisa J. 
Published: September 2019

AbstractAfter spinal cord injury (SCI) chronic inflammation hampers regeneration. Influencing the local microenvironment after SCI may provide a strategy to modulate inflammation and the immune response. The objectives of this work were to determine whether bone or spinal cord derived ECM hydrogels can deliver human mesenchymal stem cells from the apical papilla (SCAP) to reduce local inflammation and provide a regenerative microenvironment. Bone hydrogels (8 and 10 mg/ml, B8 and B10) and spinal cord hydrogels (8 mg/ml, S8) supplemented with fibrin possessed a gelation rate and a storage modulus compatible with spinal cord implantation. S8 and B8 impact on the expression of anti and pro-inflammatory cytokines (Arg1, Nos2, Tnf) in LPS treated microglial cells were assessed using solubilised and solid hydrogel forms. S8 significantly reduced the Nos2/Arg1 ratio and solubilised B8 significantly reduced Tnf and increased Arg1 whereas solid S8 and B8 did not impact inflammation in microglial cells. SCAP incorporation within ECM hydrogels did not impact upon SCAP immunoregulatory properties, with significant downregulation of Nos2/Arg1 ratio observed for all SCAP embedded hydrogels. Tnf expression was reduced with SCAP embedded in B8, reflecting the gene expression observed with the innate hydrogel. Thus, ECM hydrogels are suitable vehicles to deliver SCAP due to their physical properties, preservation of SCAP viability and immunomodulatory capacity.
Precision Vascular Delivery of Agrochemicals with Micromilled Microneedles (µMMNs)
Authors: Kundu Avra , Nogueira Campos Maria Gabriela , Santra Swadeshmukul , Rajaraman Swaminathan 
Published: September 2019

AbstractWe demonstrate use of makerspace techniques involving subtractive microtechnologies to fabricate micromilled microneedles (µMMNs) of stainless steel (SS) for precise delivery of agrochemicals into vascular bundles of plant tissue. Precision delivery is of immense importance for systemic pathogen control in specific areas of plant tissue. Optimization of the micromilling allows for selective removal of SS at the microscale and the microfabrication of a 5 × 5 array of µMMNs having both base width and height of 500 µm to enable precise puncture into the stem of citrus saplings. Atomic Absorption Spectroscopy reveals up to 7.5× increase in the uptake of a therapeutic cargo while Scanning Electron Microscopy reveals that specific sites of the vascular bundle; either xylem or the phloem can be uniquely targeted with customized µMMNs. Such rapid and cost-effective customization with intricate designs along with scalability is enabled by makerspace microfabrication. Additionally, a 19 × 20 array of micromilled mesoneedles has been fabricated and affixed to a paint roller as an applicator system for real-world field testing outside the laboratory. Initial results indicate reliable behavior of the applicator system and the technique can be applied to the systemic delivery of agrochemicals while conserving the loss of the agrochemical with increased application efficiency.
An Effective Way for Simulating Oceanic Turbulence Channel on the Beam Carrying Orbital Angular Momentum
Authors: Pan Sunxiang , Wang Le , Wang Wennai , Zhao Shengmei 
Published: September 2019

AbstractIn this paper, we present an effective way for simulating oceanic turbulence channel on the beam carrying orbital angular momentum (OAM). The influence caused by oceanic turbulence channel on the phase and intensity of the propagation beam is equivalent to that the beam passing through several individual phase screens generated by power spectrum inversion method at regular intervals. A modified subharmonic compensation method is then further balance the phase screen for the losses of lower frequency components in the power spectrum inversion method. The feasibility is verified by the theoretical phase structure function and the propagation characteristics of an OAM beam in underwater environment. The results show that the phase structure function and the propagation characteristics of the OAM beam evaluated by the phase screen model all coincide with those theoretical results at high spatial frequency. Simultaneously, the low frequency components could be effectively compensated by the modified subharmonic method. With the increase of the subharmonic order and sample level, the performance evaluated by the phase screen model are closer to the theoretical ones. It has provided an effective way for simulating oceanic turbulence channel for the underwater optical communications.
A variant of the castor zinc finger 1 (CASZ1) gene is differentially associated with the clinical classification of chronic venous disease
Authors: Jones Gregory T. , Marsman Judith , Pardo Luba M. , Nijsten Tamar , De Maeseneer Marianne , Phillips Vicky , Lynch-Sutherland Chi , Horsfield Julia , Krysa Jolanda , van Rij Andre M. 
Published: September 2019

AbstractRecent reports have suggested a reproducible association between the rs11121615 SNP, located within an intron of the castor zinc finger 1 (CASZ1) gene, and varicose veins. This study aimed to determine if this variant is also differentially associated with the various clinical classifications of chronic venous disease (CVD). The rs11121615 SNP was genotyped in two independent cohorts from New Zealand (n = 1876 controls /1606 CVD cases) and the Netherlands (n = 1626/2966). Participants were clinically assessed using well-established CVD criteria. The association between the rs11121615 C-allele and varicose veins was validated in both cohorts. This was strongest in those with higher clinical severity classes and was not significant in those with non-varicose vein CVD. Functional analysis of the rs11121615 variant demonstrated that the risk allele was associated with increased enhancer activity. This study demonstrates that the CASZ1 gene associated C-allele of rs11121615 has a significant, reproducible, association with CVD (CEAP C ≥ 2 meta-odds ratio 1.31, 95% CI 1.27–1.34, P = 1 × 10−98, PHet = 0.25), but not with non-varicose vein (CEAP C1, telangiectasia or reticular veins) forms of venous disease. The effect size of this association therefore appears to be susceptible to influence by phenotypic heterogeneity, particularly if a cohort includes a large number of cases with lower severity CVD.
Identification of an adhesive interface for the non-clustered δ1 protocadherin-1 involved in respiratory diseases
Authors: Modak Debadrita , Sotomayor Marcos 
Published: September 2019

AbstractCadherins form a large family of calcium-dependent adhesive proteins involved in morphogenesis, cell differentiation, and neuronal connectivity. Non-clustered δ1 protocadherins form a cadherin subgroup of proteins with seven extracellular cadherin (EC) repeats and cytoplasmic domains distinct from those of classical cadherins. Non-clustered δ1 protocadherins mediate homophilic adhesion and have been implicated in various diseases including asthma, autism, and cancer. Here we present X-ray crystal structures of human Protocadherin-1 (PCDH1), a δ1-protocadherin member essential for New World Hantavirus infection that is typically expressed in the brain, airway epithelium, skin keratinocytes, and lungs. The structures suggest a binding mode that involves antiparallel overlap of repeats EC1 to EC4. Mutagenesis combined with binding assays and biochemical experiments validated this mode of adhesion. Overall, these results reveal the molecular mechanism underlying adhesiveness of PCDH1 and δ1-protocadherins, also shedding light on PCDH1’s role in maintaining airway epithelial integrity, the loss of which causes respiratory diseases.
Differential cell-type dependent brain state modulations of sensory representations in the non-lemniscal mouse inferior colliculus
Authors: Chen Chenggang , Song Sen 
Published: September 2019

AbstractSensory responses of the neocortex are strongly influenced by brain state changes. However, it remains unclear whether and how the sensory responses of the midbrain are affected. Here we addressed this issue by using in vivo two-photon calcium imaging to monitor the spontaneous and sound-evoked activities in the mouse inferior colliculus (IC). We developed a method enabling us to image the first layer of non-lemniscal IC (IC shell L1) in awake behaving mice. Compared with the awake state, spectral tuning selectivity of excitatory neurons was decreased during isoflurane anesthesia. Calcium imaging in behaving animals revealed that activities of inhibitory neurons were highly correlated with locomotion. Compared with stationary periods, spectral tuning selectivity of excitatory neurons was increased during locomotion. Taken together, our studies reveal that neuronal activities in the IC shell L1 are brain state dependent, whereas the brain state modulates the excitatory and inhibitory neurons differentially.
Photoacoustics of core–shell nanospheres using comprehensive modeling and analytical solution approach
Authors: Shahbazi Khosro , Frey Wolfgang , Chen Yun-Sheng , Aglyamov Salavat , Emelianov Stanislav 
Published: September 2019

AbstractPhotoacoustic visualization of nanoparticles is capable of high contrast imaging at depth greater than that of traditional optical imaging techniques. Identifying the impact of various parameters on the photoacoustic signal is crucial in the design of effective medical imaging and diagnostics. Here, we develop a complete model of Fourier heat conduction incorporating the interfacial thermal resistance and photoacoustic equation for core-shell nanospheres in a fluid under nanosecond pulsed laser illumination. An analytical solution is obtained, elucidating the contribution of each region (core, shell, or the fluid) in the generation of the photoacoustic signal. The model reveals that the sharper the laser pulse temporal waveform is, the higher the sensitivity of the generated photoacoustic signal will be to the interfacial thermal resistance, and, thus, the higher the possibility of photoacoustic signal amplification will be using silica-coating. The comprehensive model and adopted analytical solution reveal the underlying physics of the photoacoustic signal generation form core-shell nanosphere systems.
J-PLUS: photometric calibration of large-area multi-filter surveys with stellar and white dwarf loci
Authors: López-Sanjuan C. , Varela J. , Cristóbal-Hornillos D. , Vázquez Ramió H. , Carrasco J. M. , Tremblay P.-E. , Whitten D. D. , Placco V. M. , Marín-Franch A. , Cenarro A. J. , Ederoclite A. , Alfaro E. , Coelho P. R. T. , Civera T. , Hernández-Fuertes J. , Jiménez-Esteban F. M. , Jiménez-Teja Y. , Maíz Apellániz J. , Sobral D. , Vílchez J. M. , Alcaniz J. , Angulo R. E. , Dupke R. A. , Hernández-Monteagudo C. , Mendes de Oliveira C. L. , Moles M. , Sodré L. 
Published: September 2019

Aims. We present the photometric calibration of the 12 optical passbands observed by the Javalambre Photometric Local Universe Survey (J-PLUS).
Methods. The proposed calibration method has four steps: (i) definition of a high-quality set of calibration stars using Gaia information and available 3D dust maps; (ii) anchoring of the J-PLUS gri passbands to the Pan-STARRS photometric solution, accounting for the variation in the calibration with the position of the sources on the CCD; (iii) homogenization of the photometry in the other nine J-PLUS filters using the dust de-reddened instrumental stellar locus in (𝒳 − r) versus (g − i) colours, where 𝒳 is the filter to calibrate. The zero point variation along the CCD in these filters was estimated with the distance to the stellar locus. Finally, (iv) the absolute colour calibration was obtained with the white dwarf locus. We performed a joint Bayesian modelling of 11 J-PLUS colour–colour diagrams using the theoretical white dwarf locus as reference. This provides the needed offsets to transform instrumental magnitudes to calibrated magnitudes outside the atmosphere.
Results. The uncertainty of the J-PLUS photometric calibration, estimated from duplicated objects observed in adjacent pointings and accounting for the absolute colour and flux calibration errors, are ∼19 mmag in u, J0378, and J0395; ∼11 mmag in J0410 and J0430; and ∼8 mmag in g, J0515, r, J0660, i, J0861, and z.
Conclusions. We present an optimized calibration method for the large-area multi-filter J-PLUS project, reaching 1–2% accuracy within an area of 1022 square degrees without the need for long observing calibration campaigns or constant atmospheric monitoring. The proposed method will be adapted for the photometric calibration of J-PAS, that will observe several thousand square degrees with 56 narrow optical filters.