bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bite performance surfaces of three ecologically divergent Iguanidae lizards: relationships with lower jaw bones
Authors: Simon Monique Nouailhetas , Brandt Renata , Kohlsdorf Tiana , Arnold Stevan J 
Published: April 2019

AbstractTraits that interact to perform an ecologically relevant function are expected to be under multivariate non-linear selection. Using the lower jaw morphology as a biomechanical model, we test the hypothesis that lower jaw bones of lizards are subjected to stabilizing and correlational selection, associated with mechanical advantage and maximum bite force. We used three closely related tropidurine species that differ in size, head shape and microhabitat: Eurolophosaurus nanuzae, Tropidurus hispidus and Tropidurus semitaeniatus. We predicted a common pattern of correlational selection on bones that are part of in-levers or part of the out-lever of the lower jaw. The predicted pattern was found in E. nanuzae and T. hispidus, but this could not be shown to be statistically significant. For T. semitaeniatus, we found significant disruptive selection on a contrast involving the surangular, and also significant directional selection on linear combinations of traits in all species. The results indicate that the non-linear selection on lower jaw bones does not reflect an optimum to enhance mechanical advantage in all species. Divergent functional demands and specific ecological contexts of species seem relevant in shaping patterns of selection on morphology.
Growth differentiation factor 8 regulates SMAD2/3 signaling and improves oocyte quality during porcine oocyte maturation in vitro†
Authors: Yoon Junchul David , Hwang Seon-Ung , Kim Mirae , Jeon Yubyeol , Hyun Sang-Hwan 
Published: April 2019

Abstract
Growth differentiation factor 8 (GDF8), also known as myostatin, is a member of the transforming growth factor-β (TGF-β) family and has been identified as a strong physiological regulator of muscle differentiation. Recently, the functional role of GDF8 in reproductive organs has received increased interest following its detection in the human placenta and uterus. To investigate the effects of GDF8 during porcine oocyte in vitro maturation (IVM), we assessed the quality of matured oocytes. Furthermore, we investigated the specific gene transcription and protein activation levels in oocytes and cumulus cells after IVM and subsequent embryonic development after in vitro fertilization and parthenogenetic activation. Prior to these experiments, the concentration of GDF8 in porcine follicular fluid was determined. During the entire IVM period, 1.3 ng/mL GDF8 and its signaling inhibitor SB431542 (SB) at 5 μM were added as control, SB, SB + GDF8, and GDF8 groups, respectively. Our results demonstrate that supplementation with GDF8 during porcine oocyte IVM enhanced both meiotic and cytoplasmic maturation, with altered transcriptional patterns, via activation of Sma- and Mad-related protein 2/3 (SMAD2/3). Using the pharmacological inhibitor SB431542, we demonstrated that inhibition of GDF8-induced Smad2/3 signaling reduces matured oocyte quality. In conclusion, for the first time, we demonstrated paracrine factor GDF8 in porcine follicular fluid in vivo. Furthermore, we showed that GDF8 supplementation improved mature oocyte quality by regulating p38 mitogen-activated protein kinase phosphorylation and intracellular glutathione and reactive oxygen species levels during porcine IVM.
Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice†
Authors: Shi Lin , Zhou Ting , Huang Qian , Zhang Shiyang , Li Wei , Zhang Ling , Hess Rex A , Pazour Gregory J , Zhang Zhibing 
Published: April 2019

Abstract
Intraflagellar transport protein 74 (IFT74) is a component of the core intraflagellar transport complex, a bidirectional movement of large particles along the axoneme microtubules for cilia formation. In this study, we investigated its role in sperm flagella formation and discovered that mice deficiency in Ift74 gene in male germ cells were infertile with low sperm count and immotile sperm. The few developed spermatozoa displayed misshaped heads and short tails. Transmission electron microscopy revealed abnormal flagellar axonemes in the seminiferous tubules where sperm are made. Clusters of unassembled microtubules were present in the spermatids. Testicular expression levels of IFT27, IFT57, IFT81, IFT88, and IFT140 proteins were significantly reduced in the conditional Ift74 mutant mice, with the exception of IFT20 and IFT25. The levels of outer dense fiber 2 and sperm-associated antigen 16L proteins were also not changed. However, the processed A-Kinase anchor protein, a major component of the fibrous sheath, a unique structure of sperm tail, was significantly reduced. Our study demonstrates that IFT74 is essential for mouse sperm formation, probably through assembly of the core axoneme and fibrous sheath, and suggests that IFT74 may be a potential genetic factor affecting male reproduction in man.
CXCR4 signaling at the ovine fetal–maternal interface regulates vascularization, CD34+ cell presence, and autophagy in the endometrium†
Authors: Runyan Cheyenne L , McIntosh Stacia Z , Maestas Marlie M , Quinn Kelsey E , Boren Ben P , Ashley Ryan L 
Published: April 2019

Abstract
Placenta development is characterized by extensive angiogenesis and vascularization but if these processes are compromised placental dysfunction occurs, which is the underlying cause of pregnancy complications such as preeclampsia and intrauterine growth restriction. Dysregulation of placental angiogenesis has emerged as one of the main pathophysiological features in the development of placental insufficiency and its clinical consequences. The signaling axis initiated by chemokine ligand 12 (CXCL12) and its receptor CXCR4 stimulates angiogenesis in other tissues, and may be central to placental vascularization. We hypothesized that CXCL12-CXCR4 signaling governs the pro-angiogenic placental microenvironment by coordinating production of central angiogenic factors and receptors and regulates endometrial cell survival essential for placental function and subsequent fetal longevity. The CXCR4 antagonist, AMD3100, was used to elucidate the role of CXCL12-CXCR4 signaling regarding uteroplacental vascular remodeling at the fetal–maternal interface. On day 12 postbreeding, osmotic pumps were surgically installed and delivered either AMD3100 or PBS into the uterine lumen ipsilateral to the corpus luteum. On day 20, endometrial tissues were collected, snap-frozen in liquid nitrogen, and uterine horn cross sections preserved for immunofluorescent analysis. In endometrium from ewes receiving AMD3100 infusion, the abundance of select angiogenic factors was diminished, while presence of CD34+ cells increased compared to control ewes. Ewes receiving AMD3100 infusion also exhibited less activation of Akt/mTOR signaling, and elevated LC3B-II, a marker of cellular autophagy in endometrium. This study suggests that CXCL12-CXCR4 signaling governs placental homeostasis by serving as a critical upstream mediator of vascularization and cell viability, thereby ensuring appropriate placental development.
Genome-wide copy number variation analysis identified ANO1 as a novel oncogene and prognostic biomarker in esophageal squamous cell cancer
Authors: Yu Yue , Cao Jing , Wu Weibing , Zhu Quan , Tang Yu , Zhu Chengxiang , Dai Juncheng , Li Zhihua , Wang Jun , Xue Lei , Zhen Fuxi , Liu Jinyuan , Huang Chenjun , Zhao Fei , Zhou Yue , Wen Wei , Pan Xianglong , Wei Haixing , Zhu Yining , He Yaozhou , Que Jun , Wang Wei , Luo Jinhua , Xu Jing , Chen Liang 
Published: April 2019

Abstract
Copy number variations (CNVs) represent one of the most common genomic alterations. This study aimed to evaluate the roles of genes within highly aberrant genome regions in the prognosis of esophageal squamous cell cancer (ESCC). Exome sequencing data from 81 paired ESCC tissues were used to screen aberrant genomic regions. The associations between CNVs and gene expression were evaluated using gene expression data from the same individuals. Then, an RNA expression array profile from 119 ESCC samples was adopted for differential gene expression and prognostic analyses. Two independent ESCC cohorts with 315 subjects were further recruited to validate the prognostic value using immunohistochemistry tests. Finally, we explored the potential mechanism of our identified novel oncogene in ESCC. In total, 2003 genes with CNVs were observed, of which 76 genes showed recurrent CNVs in more than three samples. Among them, 32 genes were aberrantly expressed in ESCC tumor tissues and statistically correlated with CNVs. Strikingly, 4 (CTTN, SHANK2, INPPL1 and ANO1) of the 32 genes were significantly associated with the prognosis of ESCC patients. Patients with a positive expression of ANO1 had a poorer prognosis than ANO1 negative patients (overall survival rate: 42.91% versus 26.22% for ANO1−/+ samples, P < 0.001). Functionally, ANO1 promoted ESCC cell proliferation, migration and invasion by activating transforming growth factor-β pathway. Knockdown of ANO1 significantly inhibited tumor progression in vitro and in vivo. In conclusion, ANO1 is a novel oncogene in ESCC and may serve as a prognostic biomarker for ESCC.
Bitter-Induced Salivary Proteins Increase Detection Threshold of Quinine, But Not Sucrose
Authors: Martin Laura E , Kay Kristen E , Torregrossa Ann-Marie 
Published: April 2019

AbstractExposures to dietary tannic acid (TA, 3%) and quinine (0.375%) upregulate partially overlapping sets of salivary proteins which are concurrent with changes in taste-driven behaviors, such as rate of feeding and brief access licking to quinine. In addition, the presence of salivary proteins reduces chorda tympani responding to quinine. Together these data suggest that salivary proteins play a role in bitter taste. We hypothesized that salivary proteins altered orosensory feedback to bitter by decreasing sensitivity to the stimulus. To that end, we used diet exposure to alter salivary proteins, then assessed an animal’s ability to detect quinine, using a 2-response operant task. Rats were asked to discriminate descending concentrations of quinine from water in a modified forced-choice paradigm, before and after exposure to diets that alter salivary protein expression in a similar way (0.375% quinine or 3% TA), or 1 of 2 control diets. Control animals received either a bitter diet that does not upregulate salivary proteins (4% sucrose octaacetate), or a nonbitter diet. The rats exposed to salivary protein-inducing diets significantly decreased their performance (had higher detection thresholds) after diet exposure, whereas rats in the control conditions did not alter performance after diet exposure. A fifth group of animals were trained to detect sucrose before and after they were maintained on the 3% TA diet. There was no significant difference in performance, suggesting that these shifts in threshold are stimulus specific rather than task specific. Taken together, these results suggest that salivary proteins reduce sensitivity to quinine.
Efficacy and Safety of Ascending Dosages of Moxidectin and Moxidectin-albendazole Against Trichuris trichiura in Adolescents: A Randomized Controlled Trial
Authors: Keller Ladina , Palmeirim Marta S , Ame Shaali M , Ali Said M , Puchkov Maxim , Huwyler Jörg , Hattendorf Jan , Keiser Jennifer 
Published: April 2019

Abstract

Background
Preventive chemotherapy is the main strategy to control soil-transmitted helminth (STH) infections. Albendazole and mebendazole are ubiquitously used, but they are not sufficiently effective against Trichuris trichiura. Moxidectin might be a useful addition to the small drug armamentarium. However, the optimal dosage of moxidectin alone and in combination with albendazole against T. trichiura and other STHs has not yet been determined.

Methods
A Phase II, randomized, placebo-controlled, dose-finding trial was conducted in 2 secondary schools on Pemba Island, Tanzania. Using a computer-generated list, T. trichiura–infected adolescents were randomly assigned to 7 treatment arms: 8, 16, or 24 mg of moxidectin monotherapy; 8, 16, or 24 mg of moxidectin plus 400 mg of albendazole combination therapy; or placebo. The primary outcome was cure rate (CR) against T. trichiura, analyzed 13 to 20 days after treatment by quadruple Kato-Katz thick smears.

Results
A total of 290 adolescents were enrolled (41 or 42 per arm). CRs against T. trichiura were 43, 46, and 44% for 8, 16, and 24 mg of moxidectin alone, respectively; 60, 62, and 66% for the same moxidectin dosages plus 400 mg of albendazole, respectively; and 12% for placebo. The moxidectin-albendazole arms also revealed higher CRs and egg reduction rates against hookworm than the monotherapy arms. Moxidectin and its combination with albendazole were well tolerated.

Conclusions
Moxidectin-albendazole is superior to moxidectin. There is no benefit of using doses above 8 mg, which is the recommended dose for onchocerciasis. The moxidectin-albendazole combination of 8 mg plus 400 mg should be investigated further to develop recommendations for appropriate control of STH infections.

Clinical Trials Registration
NCT03501251.
A Nationwide Spatiotemporal Analysis of Syphilis Over 21 Years and Implications for Prevention and Control in China
Authors: Tao Yusha , Chen Marcus Y , Tucker Joseph D , Ong Jason J , Tang Weiming , Wong Ngai Sze , Chu Minjie , Zhuang Xun , Fairley Christopher K , Zhang Lei 
Published: April 2019

Abstract
Syphilis control programs have been scaled up due to the substantial burden in China. We analyzed syphilis incidence according to demographic, spatiotemporal, and economic factors. The increasing latent syphilis diagnoses and declining congenital syphilis suggest the effectiveness of scale-up screening. However, primary and secondary cases persist, especially in inland provinces.
Prophylactic Versus Therapeutic Inoculation Treatments for Resistance to Influence
Authors: Compton Josh 
Published: April 2019

AbstractOne of the most significant departures from conventional inoculation theory is its intentional application for individuals already “infected”—that is, inoculation not as a preemptive strategy to protect existing positions from future challenges, but instead, inoculation as a means to change a position (e.g., from negative to positive) and to protect the changed position against future challenges. The issue is important for persuasion scholarship in general, as theoretical boundary conditions help at each stage of persuasion research development, serving as a guide for literature review, analysis, synthesis, research design, interpretation, theory building, and so on. It is an important issue for inoculation theory and resistance to influence research, specifically, for it gets at the very heart—and name and foundation—of inoculation theory. This article offers a theoretical analysis of inoculation theory used as both prophylactic and therapeutic interventions and concludes with a set of recommendations for inoculation theory scholarship moving forward.
Rhetorical Arbitrage: The Rhetoric of the Sharing Economy
Authors: Duerringer Christopher Michael 
Published: April 2019

AbstractThis article extends the growing conversation on the rhetoric of economics through a critical analysis of the rhetoric of the “sharing economy” firm Uber. After reconfiguring the economic concept of arbitrage from a rhetorical perspective, I demonstrate the means by which “ride sharing” services exploit their customers and workers. Ultimately, I contend that Uber’s rhetoric works by deploying the language of the private sphere in order that the company can operate like a taxi company even as it evades the obligations that ordinarily attach to providing taxi service to the public.
Work and Employment Characteristics of Deaf and Hard-of-Hearing Adults
Authors: Dammeyer Jesper , Crowe Kathryn , Marschark Marc , Rosica Mark 
Published: April 2019

AbstractStudies have reported a higher level of unemployment among deaf and hard-of-hearing (DHH) adults compared to adults without hearing loss. However, little is known about factors explaining success in the labor market. This study presents the analysis of two data sets. The first was drawn from a survey of 804 DHH adults in Denmark. The second was a survey completed by 190 DHH adults with post-secondary qualification in the United States. In the Danish sample, among the spoken language users, higher ages, higher level of educational attainment, and no additional disabilities were associated with having a job. Among the sign language users, higher ages, higher level of educational attainment, earlier ages at hearing loss diagnosis, and not using cochlear implants were associated with having a job. In the US sample, male gender and better sign language skills were associated with having a job.
Transcatheter aortic valve implantation vs. surgical aortic valve replacement for treatment of symptomatic severe aortic stenosis: an updated meta-analysis
Authors: Siontis George C M , Overtchouk Pavel , Cahill Thomas J , Modine Thomas , Prendergast Bernard , Praz Fabien , Pilgrim Thomas , Petrinic Tatjana , Nikolakopoulou Adriani , Salanti Georgia , Søndergaard Lars , Verma Subodh , Jüni Peter , Windecker Stephan 
Published: April 2019

Abstract

Aims
Owing to new evidence from randomized controlled trials (RCTs) in low-risk patients with severe aortic stenosis, we compared the collective safety and efficacy of transcatheter aortic valve implantation (TAVI) vs. surgical aortic valve replacement (SAVR) across the entire spectrum of surgical risk patients.

Methods and results
The meta-analysis is registered with PROSPERO (CRD42016037273). We identified RCTs comparing TAVI with SAVR in patients with severe aortic stenosis reporting at different follow-up periods. We extracted trial, patient, intervention, and outcome characteristics following predefined criteria. The primary outcome was all-cause mortality up to 2 years for the main analysis. Seven trials that randomly assigned 8020 participants to TAVI (4014 patients) and SAVR (4006 patients) were included. The combined mean STS score in the TAVI arm was 9.4%, 5.1%, and 2.0% for high-, intermediate-, and low surgical risk trials, respectively. Transcatheter aortic valve implantation was associated with a significant reduction of all-cause mortality compared to SAVR {hazard ratio [HR] 0.88 [95% confidence interval (CI) 0.78–0.99], P = 0.030}; an effect that was consistent across the entire spectrum of surgical risk (P-for-interaction = 0.410) and irrespective of type of transcatheter heart valve (THV) system (P-for-interaction = 0.674). Transcatheter aortic valve implantation resulted in lower risk of strokes [HR 0.81 (95% CI 0.68–0.98), P = 0.028]. Surgical aortic valve replacement was associated with a lower risk of major vascular complications [HR 1.99 (95% CI 1.34–2.93), P = 0.001] and permanent pacemaker implantations [HR 2.27 (95% CI 1.47–3.64), P &lt; 0.001] compared to TAVI.

Conclusion
Compared with SAVR, TAVI is associated with reduction in all-cause mortality and stroke up to 2 years irrespective of baseline surgical risk and type of THV system.
Protein-based cardiogenic shock patient classifier
Authors: Rueda Ferran , Borràs Eva , García-García Cosme , Iborra-Egea Oriol , Revuelta-López Elena , Harjola Veli-Pekka , Cediel Germán , Lassus Johan , Tarvasmäki Tuukka , Mebazaa Alexandre , Sabidó Eduard , Bayés-Genís Antoni 
Published: April 2019

Abstract

Aims
Cardiogenic shock (CS) is associated with high short-term mortality and a precise CS risk stratification could guide interventions to improve patient outcome. Here, we developed a circulating protein-based score to predict short-term mortality risk among patients with CS.

Methods and results
Mass spectrometry analysis of 2654 proteins was used for screening in the Barcelona discovery cohort (n = 48). Targeted quantitative proteomics analyses (n = 51 proteins) were used in the independent CardShock cohort (n = 97) to derive and cross-validate the protein classifier. The combination of four circulating proteins (Cardiogenic Shock 4 proteins—CS4P), discriminated patients with low and high 90-day risk of mortality. CS4P comprises the abundances of liver-type fatty acid-binding protein, beta-2-microglobulin, fructose-bisphosphate aldolase B, and SerpinG1. Within the CardShock cohort used for internal validation, the C-statistic was 0.78 for the CardShock risk score, 0.83 for the CS4P model, and 0.84 (P = 0.033 vs. CardShock risk score) for the combination of CardShock risk score with the CS4P model. The CardShock risk score with the CS4P model showed a marked benefit in patient reclassification, with a net reclassification improvement (NRI) of 0.49 (P = 0.020) compared with CardShock risk score. Similar reclassification metrics were observed in the IABP-SHOCK II risk score combined with CS4P (NRI =0.57; P = 0.032). The CS4P patient classification power was confirmed by enzyme-linked immunosorbent assay (ELISA).

Conclusion
A new protein-based CS patient classifier, the CS4P, was developed for short-term mortality risk stratification. CS4P improved predictive metrics in combination with contemporary risk scores, which may guide clinicians in selecting patients for advanced therapies.
The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs
Authors: Jacobsen Thomas , Liao Chunyu , Beisel Chase L 
Published: April 2019

ABSTRACT
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) nuclease Acidaminococcus sp. Cas12a (AsCas12a, also known as AsCpf1) has become a popular alternative to Cas9 for genome editing and other applications. AsCas12a has been associated with a TTTV protospacer-adjacent motif (PAM) as part of target recognition. Using a cell-free transcription-translation (TXTL)-based PAM screen, we discovered that AsCas12a can also recognize GTTV and, to a lesser degree, GCTV motifs. Validation experiments involving DNA cleavage in TXTL, plasmid clearance in Escherichia coli, and indel formation in mammalian cells showed that AsCas12a was able to recognize these motifs, with the GTTV motif resulting in higher cleavage efficiency compared to the GCTV motif. We also observed that the -5 position influenced the activity of DNA cleavage in TXTL and in E. coli, with a C at this position resulting in the lowest activity. Together, these results show that wild-type AsCas12a can recognize non-canonical GTTV and GCTV motifs and exemplify why the range of PAMs recognized by Cas nucleases are poorly captured with a consensus sequence.
Potential for Sugar Maple to Provide High-Quality Sawlog Trees at the Northern Edge of Its Range
Authors: Guillemette François , Bédard Steve 
Published: April 2019

AbstractThe management of sugar maple (Acer saccharum) at the northern edge of its range is mainly oriented toward timber production, from trees of higher grades. However, both the quality of mature trees in natural stands and how the quality may vary depending on the silvicultural treatment are unknown, especially under northern conditions. The objective of this study was to describe the variation in stem quality of mature maple trees (diameter >33 cm) according to climatic, geographic or soil variables, and to evaluate the effects of a first selection cutting cycle on this quality. Annual temperature (1.7–4.1° C) was the most important variable explaining differences in the proportion of higher-grade trees, with a 16 percent gain associated with every additional increase in degrees Celsius. The practice of a first selection cutting was associated with an 11 percent gain in this proportion. Although the actual proportion of high-quality trees was below 35 percent on the coolest sites, a proper tree selection through silviculture could likely improve this proportion in future decades, whereas the potential effects of climate change are unclear.