bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute dietary zinc deficiency in rats exacerbates myocardial ischaemia–reperfusion injury through depletion of glutathione
Authors: Skene Karen , Walsh Sarah K. , Okafor Oronne , Godsman Nadine , Barrows Charlotte , Meier Philip , Gordon Margaret J. , Beattie John H. , Wainwright Cherry L. 
Published: February 2019

AbstractZn plays an important role in maintaining the anti-oxidant status within the heart and helps to counter the acute redox stress that occurs during myocardial ischaemia and reperfusion. Individuals with low Zn levels are at greater risk of developing an acute myocardial infarction; however, the impact of this on the extent of myocardial injury is unknown. The present study aimed to compare the effects of dietary Zn depletion with in vitro removal of Zn (N,N,N′,N′-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine (TPEN)) on the outcome of acute myocardial infarction and vascular function. Male Sprague–Dawley rats were fed either a Zn-adequate (35 mg Zn/kg diet) or Zn-deficient (&lt;1 mg Zn/kg diet) diet for 2 weeks before heart isolation. Perfused hearts were subjected to a 30 min ischaemia/2 h reperfusion (I/R) protocol, during which time ventricular arrhythmias were recorded and after which infarct size was measured, along with markers of anti-oxidant status. In separate experiments, hearts were challenged with the Zn chelator TPEN (10 µm) before ischaemia onset. Both dietary and TPEN-induced Zn depletion significantly extended infarct size; dietary Zn depletion was associated with reduced total cardiac glutathione (GSH) levels, while TPEN decreased cardiac superoxide dismutase 1 levels. TPEN, but not dietary Zn depletion, also suppressed ventricular arrhythmias and depressed vascular responses to nitric oxide. These findings demonstrate that both modes of Zn depletion worsen the outcome from I/R but through different mechanisms. Dietary Zn deficiency, resulting in reduced cardiac GSH, is the most appropriate model for determining the role of endogenous Zn in I/R injury.
Stance-taking and participation framework in museum commenting platforms: On subjects, objects, authors, and principals
Authors: Noy Chaim , Hamo Michal 
Published: February 2019

AbstractThe realization of subjectivity through language use is a key concern of sociolinguistic research. We argue for examining it by juxtaposing Goffman's participation framework and Du Bois’ stance triangle. We focus on museums’ commenting platforms as ‘stance-rich’ media (Du Bois 2007:151) by examining the communicative affordances of a visitor book at the Florida Holocaust Museum and analyzing museumgoers’ texts (3,064). These texts are homogeneous in their morally indignant evaluation of the Holocaust and their alignment with the museum, but heterogeneous in positioning and participation framework. Museumgoers’ texts are highly responsive to the setting and previous discourses of the museum and include patterns of shared authorship and principalship. These patterns allow museumgoers to construct shared, institutionally mandated, subjectivities while maintaining personal commitment. These findings contribute to our understanding of the construction of subjectivity as inherently dialogic, and as involving the purposeful use of a multiplicity of contextual resources. (Participation framework, stance triangle, repetitions, principalship, commitment, Holocaust, museums, visitor books)
A unificationist defence of revealed preferences
Authors: Vredenburgh Kate 
Published: February 2019

AbstractRevealed preference approaches to modelling agents’ choices face two seemingly devastating explanatory objections. The no self-explanation objection imputes a problematic explanatory circularity to revealed preference approaches, while the causal explanation objection argues that, all things equal, a scientific theory should provide causal explanations, but revealed preference approaches decidedly do not. Both objections assume a view of explanation, the constraint-based view, that the revealed preference theorist ought to reject. Instead, the revealed preference theorist should adopt a unificationist account of explanation, allowing her to escape the two explanatory problems discussed in this paper.
QUANTILE-BASED STUDY OF (DYNAMIC) INACCURACY MEASURES
Authors: Kayal Suchandan , Moharana Rajesh , Sunoj S. M. 
Published: February 2019

AbstractIn the present communication, we introduce quantile-based (dynamic) inaccuracy measures and study their properties. Such measures provide an alternative approach to evaluate inaccuracy contained in the assumed statistical models. There are several models for which quantile functions are available in tractable form, though their distribution functions are not available in explicit form. In such cases, the traditional distribution function approach fails to compute inaccuracy between two random variables. Various examples are provided for illustration purpose. Some bounds are obtained. Effect of monotone transformations and characterizations are provided.
Trends of hospitalisations rates in a cohort of HIV-infected persons followed in an Italian hospital from 1998 to 2016
Authors: Bellino S. , Borghetti A. , Lombardi F. , Camoni L. , Ciccullo A. , Baldin G. , Belmonti S. , Moschese D. , Lamonica S. , Cauda R. , Pezzotti P. , Di Giambenedetto S. 
Published: February 2019

AbstractHere we evaluated hospitalisation rates and associated risk factors of human immunodeficiency virus (HIV)-infected individuals who were followed up in an Italian reference hospital from 1998 to 2016. Incidence rates (IR) of hospitalisations were calculated for five study periods from 1998 to 2016. The random-effects Poisson regression model was used to assess risk factors for hospitalisation including demographic and clinical characteristics. To consider that more events may occur for the same subject, multiple failure-time data analysis was also performed for selected causes using the Cox proportional hazards model. We evaluated 2031 patients. During 13 173 person-years (py) of follow-up, 3356 hospital admissions were carried out for 756 patients (IR: 255 per 1000 py). IR decreased significantly over the study period, from 634 in 1998–2000 to 126 per 1000 py in 2013–2016. Major declines were detected for AIDS-defining events, non-HIV/AIDS-related infections and neurological diseases. Older age, female sex, longer HIV duration and HCV coinfection were associated with a higher hospitalisation risk, whereas higher CD4 nadir and antiretroviral therapy were associated with a reduced risk. Influence of advanced HIV disease markers declined over time. Hospitalisation rates decreased during the study period in most causes. The relative weight of hospitalisations for non-AIDS-related tumours, cardiovascular, respiratory and kidney diseases increased during the study period, whereas those for AIDS-defining events declined.
Skin and soft tissue infections and current antimicrobial prescribing practices in Australian aged care residents
Authors: Bennett N. J. , Imam N. , Ingram R. J. , James R. S. , Buising K. L. , Bull A. L. , Chen C. S. , Thursky K. A. , Worth L. J. 
Published: February 2019

AbstractTo determine the burden of skin and soft tissue infections (SSTI), the nature of antimicrobial prescribing and factors contributing to inappropriate prescribing for SSTIs in Australian aged care facilities, SSTI and antimicrobial prescribing data were collected via a standardised national survey. The proportion of residents prescribed ⩾1 antimicrobial for presumed SSTI and the proportion whose infections met McGeer et al. surveillance definitions were determined. Antimicrobial choice was compared to national prescribing guidelines and prescription duration analysed using a negative binomial mixed-effects regression model. Of 12 319 surveyed residents, 452 (3.7%) were prescribed an antimicrobial for a SSTI and 29% of these residents had confirmed infection. Topical clotrimazole was most frequently prescribed, often for unspecified indications. Where an indication was documented, antimicrobial choice was generally aligned with recommendations. Duration of prescribing (in days) was associated with use of an agent for prophylaxis (rate ratio (RR) 1.63, 95% confidence interval (CI) 1.08–2.52), PRN orders (RR 2.10, 95% CI 1.42–3.11) and prescription of a topical agent (RR 1.47, 95% CI 1.08–2.02), while documentation of a review or stop date was associated with reduced duration of prescribing (RR 0.33, 95% CI 0.25–0.43). Antimicrobial prescribing for SSTI is frequent in aged care facilities in Australia. Methods to enhance appropriate prescribing, including clinician documentation, are required.
Social and economic influences on infant and child feeding practices in a Marshallese community
Authors: Ayers Britni L , Shreve Marilou D , Scott Allison L , Seaton Victoria A , Johnson Kelly V , Hawley Nicola L , Rowland Brett , Moore Ramey , McElfish Pearl A 
Published: February 2019

AbstractObjectiveThe present study aimed to examine the key influences on infant and child feeding practices among a Marshallese community at each social ecological level. It is the first study to examine the key influences on infant and child feeding practices with Marshallese immigrant women in the USA and helps fill a gap in the previous literature that has included other immigrant women.DesignCommunity-based participatory research design with twenty-seven participants taking part in four qualitative focus groups.SettingThe study took place within the Marshallese community in Arkansas, USA.ParticipantsParticipants included Marshallese women with children aged 1–3 years and/or caregivers. Caregivers were defined as someone other than the parent who cares for children. Caregivers were often older women in the Marshallese community.ResultsThere were five primary themes within multiple levels of the Social Ecological Model. At the intrapersonal level, mothers’ and caregivers’ autonomy emerged. At the interpersonal level, child-led and familial influences emerged. At the organizational level, health-care provider influences emerged; and at the policy level, the Special Supplemental Nutrition Program for Women, Infants, and Children emerged as the most salient influence.ConclusionsMarshallese immigrant women’s infant and child feeding practices are influenced at intrapersonal, interpersonal, organizational and policy levels. Understanding these multidimensional influences is necessary to inform the creation of culturally tailored interventions to reduce health disparities within the Marshallese community.
On Energy and Turbulent Mixing in the Thermocline
Authors: Dewar W. K. , McWilliams J. C. 
Published: February 2019

AbstractA new method for computing the rate at which turbulent mixing builds potential energy in the ocean is described. The traditional approach has focused on the rate of change of the background potential energy associated with an adiabatically leveled state. We argue that when examining mixing events, so‐called “Thorpe” sorting yields a useful and local measure of diabatically generated potential energy and exhibits some advantages relative to adiabatic leveling. Among these, the open question about the leveling domain is avoided, the fate of kinetic energy during a mixing event is clearly defined, and the computational load associated with the leveling is relieved. The resultant kinetic energy equation leads to a natural definition of mixing efficiency and turbulent diffusivity in terms of sign definite viscous and diffusive contributions. Applications to 2‐D Kelvin Helmholtz instability demonstrate the utility of the procedure. We find an integrated efficiency of ≈ 0.15 for a Prandtl number of 1, and of ≈ 0.08 for a Prandtl number of 10. The larger is comparable to the classical value of 0.2 used frequently by the mixing community and smaller than that found in some recent simulations.
Study of Cathepsin B inhibition in VEGFR TKI treated human renal cell carcinoma xenografts
Authors: Chen Chun-Hau , Bhasin Swati , Khanna Prateek , Joshi Mukta , Joslin Patrick MN. , Saxena Ruchi , Amin Seema , Liu Suhu , Sindhu Shreya , Walker Sarah R. , Catalano Paul , Frank David A. , Alper Seth L. , Bhasin Manoj , Bhatt Rupal S. 
Published: February 2019

AbstractSeveral therapeutic options are available for metastatic RCC, but responses are almost never complete, and resistance to therapy develops in the vast majority of patients. Consequently, novel treatments are needed to combat resistance to current therapies and to improve patient outcomes. We have applied integrated transcriptome and proteome analyses to identify cathepsin B (CTSB), a cysteine proteinase of the papain family, as one of the most highly upregulated gene products in established human RCC xenograft models of resistance to vascular endothelial growth factor receptor (VEGFR) tyrosine kinase inhibitors (TKI). We used established RCC models to test the significance of CTSB in the progression of renal cancer. Our evaluation of CTSB showed that stable CTSB knockdown suppressed RCC growth in vitro and in vivo. Stable over-overexpression of wild-type CTSB (CTSBwt/hi), but not of an CTSB active site mutant (CTSBN298A), rescued cell growth in CTSB knockdown cells and abolished the efficacy of VEGFR TKI treatment. Genome-wide transcriptome profiling of CTSB knockdown cells demonstrated significant effects on multiple metabolic and stem cell-related pathways, with ALDHA1A (ALDH1) as one of the most significantly downregulated genes. Importantly, survival analysis across 16 major TCGA cancers revealed that CTSB overexpression is associated with low rates of three and five year patient survival rates (P = 2.5e–08, HR = 1.4). These data strongly support a contribution of CTSB activity to RCC cell growth and tumorigenicity. They further highlight the promise of CTSB inhibition in development of novel combination therapies designed to improve efficacy of current TKI treatments of metastatic RCC.
Nanoparticle-based hollow microstructures formed by two-stage nematic nucleation and phase separation
Authors: Riahinasab Sheida T. , Keshavarz Amir , Melton Charles N. , Elbaradei Ahmed , Warren Gabrielle I. , Selinger Robin L. B. , Stokes Benjamin J. , Hirst Linda S. 
Published: February 2019

AbstractRapid bulk assembly of nanoparticles into microstructures is challenging, but highly desirable for applications in controlled release, catalysis, and sensing. We report a method to form hollow microstructures via a two-stage nematic nucleation process, generating size-tunable closed-cell foams, spherical shells, and tubular networks composed of closely packed nanoparticles. Mesogen-modified nanoparticles are dispersed in liquid crystal above the nematic-isotropic transition temperature (TNI). On cooling through TNI, nanoparticles first segregate into shrinking isotropic domains where they locally depress the transition temperature. On further cooling, nematic domains nucleate inside the nanoparticle-rich isotropic domains, driving formation of hollow nanoparticle assemblies. Structural differentiation is controlled by nanoparticle density and cooling rate. Cahn-Hilliard simulations of phase separation in liquid crystal demonstrate qualitatively that partitioning of nanoparticles into isolated domains is strongly affected by cooling rate, supporting experimental observations that cooling rate controls aggregate size. Microscopy suggests the number and size of internal voids is controlled by second-stage nucleation.
Counting growth factors in single cells with infrared quantum dots to measure discrete stimulation distributions
Authors: Le Phuong , Lim Sung Jun , Baculis Brian C. , Chung Hee Jung , Kilian Kristopher A. , Smith Andrew M. 
Published: February 2019

AbstractThe distribution of single-cell properties across a population of cells can be measured using diverse tools, but no technology directly quantifies the biochemical stimulation events regulating these properties. Here we report digital counting of growth factors in single cells using fluorescent quantum dots and calibrated three-dimensional deconvolution microscopy (QDC-3DM) to reveal physiologically relevant cell stimulation distributions. We calibrate the fluorescence intensities of individual compact quantum dots labeled with epidermal growth factor (EGF) and demonstrate the necessity of near-infrared emission to overcome intrinsic cellular autofluoresence at the single-molecule level. When applied to human triple-negative breast cancer cells, we observe proportionality between stimulation and both receptor internalization and inhibitor response, reflecting stimulation heterogeneity contributions to intrinsic variability. We anticipate that QDC-3DM can be applied to analyze any peptidic ligand to reveal single-cell correlations between external stimulation and phenotypic variability, cell fate, and drug response.
3D ink-extrusion additive manufacturing of CoCrFeNi high-entropy alloy micro-lattices
Authors: Kenel Christoph , Casati Nicola P. M. , Dunand David C. 
Published: February 2019

AbstractAdditive manufacturing of high-entropy alloys combines the mechanical properties of this novel family of alloys with the geometrical freedom and complexity required by modern designs. Here, a non-beam approach to additive manufacturing of high-entropy alloys is developed based on 3D extrusion of inks containing a blend of oxide nanopowders (Co3O4 + Cr2O3 + Fe2O3 + NiO), followed by co-reduction to metals, inter-diffusion and sintering to near-full density CoCrFeNi in H2. A complex phase evolution path is observed by in-situ X-ray diffraction in extruded filaments when the oxide phases undergo reduction and the resulting metals inter-diffuse, ultimately forming face-centered-cubic equiatomic CoCrFeNi alloy. Linked to the phase evolution is a complex structural evolution, from loosely packed oxide particles in the green body to fully-annealed, metallic CoCrFeNi with 99.6 ± 0.1% relative density. CoCrFeNi micro-lattices are created with strut diameters as low as 100 μm and excellent mechanical properties at ambient and cryogenic temperatures.
Lithium anode stable in air for low-cost fabrication of a dendrite-free lithium battery
Authors: Shen Xiaowei , Li Yutao , Qian Tao , Liu Jie , Zhou Jinqiu , Yan Chenglin , Goodenough John B. 
Published: February 2019

AbstractLithium metal, the ideal anode material for rechargeable batteries, suffers from the inherent limitations of sensitivity to the humid atmosphere and dendrite growth. Herein, low-cost fabrication of a metallic-lithium anode that is stable in air and plated dendrite-free from an organic-liquid electrolyte solves four key problems that have plagued the development of large-scale Li-ion batteries for storage of electric power. Replacing the low-capacity carbon anode with a safe, dendrite-free lithium anode provides a fast charge while reducing the cost of fabrication of a lithium battery, and increasing the cycle life of a rechargeable cell by eliminating the liquid-electrolyte ethylene-carbonate additive used to form a solid-electrolyte interphase passivation layer on the anode that is unstable during cycling. This solution is accomplished by formation of a hydrophobic solid-electrolyte interphase on a metallic-lithium anode that allows for handling of the treated lithium anode membrane in a standard dry room during cell fabrication.
Irreversible electroporation reverses resistance to immune checkpoint blockade in pancreatic cancer
Authors: Zhao Jun , Wen Xiaofei , Tian Li , Li Tingting , Xu Chunyu , Wen Xiaoxia , Melancon Marites P. , Gupta Sanjay , Shen Baozhong , Peng Weiyi , Li Chun 
Published: February 2019

AbstractImmunotherapy has only limited efficacy against pancreatic ductal adenocarcinoma (PDAC) due to the presence of an immunosuppressive tumor-associated stroma. Here, we demonstrate an effective modulation of that stroma by irreversible electroporation (IRE), a local ablation technique that has received regulatory approval in the United States. IRE induces immunogenic cell death, activates dendritic cells, and alleviates stroma-induced immunosuppression without depleting tumor-restraining collagen. The combination of IRE and anti-programmed cell death protein 1 (anti-PD1) immune checkpoint blockade promotes selective tumor infiltration by CD8+ T cells and significantly prolongs survival in a murine orthotopic PDAC model with a long-term memory immune response. Our results suggest that IRE is a promising approach to potentiate the efficacy of immune checkpoint blockade in PDAC.
Photosynthetic apparatus of Rhodobacter sphaeroides exhibits prolonged charge storage
Authors: Ravi Sai Kishore , Rawding Piper , Elshahawy Abdelnaby M. , Huang Kevin , Sun Wanxin , Zhao Fangfang , Wang John , Jones Michael R. , Tan Swee Ching 
Published: February 2019

AbstractPhotosynthetic proteins have been extensively researched for solar energy harvesting. Though the light-harvesting and charge-separation functions of these proteins have been studied in depth, their potential as charge storage systems has not been investigated to the best of our knowledge. Here, we report prolonged storage of electrical charge in multilayers of photoproteins isolated from Rhodobacter sphaeroides. Direct evidence for charge build-up within protein multilayers upon photoexcitation and external injection is obtained by Kelvin-probe and scanning-capacitance microscopies. Use of these proteins is key to realizing a ‘self-charging biophotonic device’ that not only harvests light and photo-generates charges but also stores them. In strong correlation with the microscopic evidence, the phenomenon of prolonged charge storage is also observed in primitive power cells constructed from the purple bacterial photoproteins. The proof-of-concept power cells generated a photovoltage as high as 0.45 V, and stored charge effectively for tens of minutes with a capacitance ranging from 0.1 to 0.2 F m−2.
Ediacaran biozones identified with network analysis provide evidence for pulsed extinctions of early complex life
Authors: Muscente A. D. , Bykova Natalia , Boag Thomas H. , Buatois Luis A. , Mángano M. Gabriela , Eleish Ahmed , Prabhu Anirudh , Pan Feifei , Meyer Michael B. , Schiffbauer James D. , Fox Peter , Hazen Robert M. , Knoll Andrew H. 
Published: February 2019

AbstractRocks of Ediacaran age (~635–541 Ma) contain the oldest fossils of large, complex organisms and their behaviors. These fossils document developmental and ecological innovations, and suggest that extinctions helped to shape the trajectory of early animal evolution. Conventional methods divide Ediacaran macrofossil localities into taxonomically distinct clusters, which may represent evolutionary, environmental, or preservational variation. Here, we investigate these possibilities with network analysis of body and trace fossil occurrences. By partitioning multipartite networks of taxa, paleoenvironments, and geologic formations into community units, we distinguish between biostratigraphic zones and paleoenvironmentally restricted biotopes, and provide empirically robust and statistically significant evidence for a global, cosmopolitan assemblage unique to terminal Ediacaran strata. The assemblage is taxonomically depauperate but includes fossils of recognizable eumetazoans, which lived between two episodes of biotic turnover. These turnover events were the first major extinctions of complex life and paved the way for the Cambrian radiation of animals.