bluuebunny/crossref_metadata_2025_split huggingface dataset:
906 PB 529 FREEZING TOLERANCE AND ACCLIMATION CAPACITY INCREASED IN CULTIVATED POTATO CROSSED TO WILD POTATO SPECIES
Authors: Weiss Laurie S. , Bamberg John B. , Palta Jiwan P. 
Published: February 2019

Solanum acaule (acl) and Solanum commersonii (cmm) represent the extremes of frost tolerance and cold acclimation ability among potato species. We have combined these species with cultivated S. tuberosum (tbr) to develop a potato with desired tuber traits and a high degree of frost tolerance. For this purpose diploid cmm was made 4x and crossed with naturally 4x acl. The F1 and F2 appear to exhibit hybrid vigor for vine growth for flowering, but none had frost tolerance greater than the parents. The F1 and F2 were crossed with S. tuberosum ssp. andigena and Katahdin via 2n eggs resulting in 6x 3-way hybrids. These hybrids were evaluated both in the field and laboratory for frost tolerance and acclimation ability. Results showed an increase of 1°C of frost tolerance and 2°C increase in cold acclimation capacity in the hybrids as compared to the sensitive tbr parents. Some of the 6x (3-way) hybrids produced significant tubers but yield and earliness needs much improvement. These results demonstrate that it should be possible to move both non acclimated freezing tolerance and cold acclimation ability from wild to cultivated species and offer exciting opportunities to enhance potato production in frost prone areas in the world.
Supported by USDA/NRI grant 91-3700-6636 to J.P.P. and J.B.B..
912 PB 156 EFFECT OF SOIL STERILIZATION WITH METHYL BROMIDE GAS ON GROWTH AND PRODUCTION OF STRAWBERRY (Fragana × ananassa) Duch
Authors: Ragab M. I. , Okasha Kh. A. 
Published: February 2019

The objective of thus work was to study the effect of soil fumigation with methyl bromide and different mulching types on growth and productivity of the strawberry cultivar Chandler. The experiment Included 8 Treatments which were the combination of 2 soil fumigation treatments × 4 mulching polyethylene types A split plot design with four replicates was adopted Soil fumigation treatments (fumigated and non fumigated) were assigned as main plots, whereas the four mulching polyethylene treatment (control, black, white and transparent) were distributed as subplots.
Results Indicated that in order to improve the vegetable growth and to increase the high yielding ability of strawberry cv. Chandler, it is recommended to fumigate soil with methyl bromide gas (50 gm/m2) and apply transparent polyethylene soil mulching
Moreover, in strawberry fields where weeds show serious problem. it is better to use the black polyethylene in controlling weeds of both the fumigated and non fumigated soils.
1004 THE DUAL ROLE OF MANNITOL AS OSMOPROTECTANT AND PHOTOASSIMILATE IN CELERY
Authors: Pharr D.M. , Stoop J.M.H. , Feusi M.E. Studer , Williamson J.D. , Massel M.O. , Conkling M.A. 
Published: February 2019

Mannitol, a six carbon sugar alcohol, is widely distributed in nature and is a major phloem-translocated photoassimilate in celery. II may also function as a compatible osmolyte providing stress tolerance. Until recently, little was known about the route of mannitol catabolism in sink tissues of higher plants. An enzyme. mannitol dehydrogenase. (MDH) that oxidizes mannitol to mannose utilizing NAD as the electron acceptor was discovered (Arch. Biochem. Biophys. 1991. 298:612-619) in “sink” tissues of celery and celeriac plants. The activity of the enzyme is inversely related to tissue mannitol concentration in various parts of celery plants suggesting a role for the enzyme in mannitol catabolism. In osmostressed celery plants, the activity of the enzyme in sink tissues decreases as mannitol accumulates.
Celery cells growing heterotrophically in suspension culture utilize either sucrose or mannitol as the sole carbon source and grow equally well on either carbohydrate. Mannitol-grown cells contain more MDI-I activity than sucrose-grown cells, and the activity of the enzyme is correlated with the rate of depletion of mannitol from the culture medium. Cells growing on mannitol contain an internal pool of mannitol but little sugar. Cells growing on sucrose contain internal sugar pools but no mannitol. Mannitol-grown cells are also more salt tolerant than cells grown on sucrose. Our laboratory is involved in studies of the physiological role of the mannitol oxidizing enzyme in regulating mannitol utilization and the role of the enzyme in regulating mannitol pool size during salt and osmostress in both celery plants and celery suspension cultures. Current studies on the molecular control of expression of the enzyme will be discussed.
1045 IMPACT OF SEED VIGOR ON PRODUCTIVITY OF VEGETABLE TRANSPLANT NURSERIES AND MECHANICAL TRANSPLANTING OPERATIONS.
Authors: Adams Steven C. 
Published: February 2019

Seed vigor has a very subtle effect on the productivity of greenhouses producing vegetable transplants, celery, cauliflower, lettuce, etc. and on todays highly mechanized automatic or semi-automatic transplanting operations. As greenhouse production technology moves from traditional bare root to plug/tray growing systems and as automatic and semi-automatic transplanting operations increase in number, the impact of poor seed vigor is realized.
Measures to mitigate the impact of poor seed vigor in the nursery are: Seed density grading; increased growing cycle in the nursery, hand culling or replanting. Measures to mitigate the impact of poor seed vigor in automatic transplanting operations: increase the number of people following the planter to replace poor vigor plants; use hand fed transplanters.
IPTASE SYNTHESIS AND LIGHT MEDIATION IN TRANSGENIC TOBACCO
Authors: Barbour Sandra L. , McMahon Margaret J. , Frett John J. , Decoteau Dennis R. 
Published: February 2019

Similarities exist between the effects of phytochrome and cytokinins on plant growth and development (e.g., chloroplast development, amaranthin synthesis. seed germination, photomorphogenesis). It is unclear, however, if and how these two systems interact.
As a beginning step to determine cytokinin-phytochrome interactions, we developed a strategy utilizing ipt -transgenic tobacco in phytochrome/light treatment investigations. The sour-cc of the ipt gene was Agrobacterium tumefaciens Ti plasmid 15955. This gene encodes for isopentenyl transferase which is an enzyme active in cytokinin biosynthesis.
Ipt -transgenic tobacco cultures (grown on MS medium supplemented with kanamycin but no plant growth regulators) were treated with end-of-day red or far-red light for 15 minutes. After 30 days of treatment, the plant tissue was harvested and either homogenized for SDS-PAGE or fixed for transmission electron microscopic analysis.
Results from immuno-gold labelling using polyclonal antibodies specific to iptase will he used to Indicate the influence of phytochrome on cytokinin activity. Also, structural changes at the ultra-cellular level will be determined.
Seasonal Fluctuations of Sugar Accumulation and the Activities of Related Enzymes Accompanying Maturation of Different Tomato Cultivars
Authors: Islam Md. Shahidul , Khan S. , Matsui T. 
Published: February 2019

Seasonal fluctuations of carbohydrate levels and compositions and the activities of related enzymes of three cultivated tomato (Lycopersicon esculentum Mill. cv. Lady First, Momotaro, and Minicarol) cultivars were examined at 45-days interval with seven different sowing in the relatively warm climate of Japan. Fruits picked on early winter to spring seasons had higher sugar concentrations compared to hot season. Fructose and glucose in nearly equal amounts were the predominant sugar in all the seasons. Sucrose was present in trace quantities, but cherry cultivar Minicarol accumulated higher levels than the other two large-fruited types.
Acid invertase (EC 3.2.1.26) was highest at red stage during December to April, while fruit matured during May to August had lowest activity. The activity levels of soluble invertase were predominant compared to cell wall-bound fraction. The sucrose synthase (EC 2.4.1.13) showed highest activity in rapidly growing fruits followed by a very low activity with fruit maturation. Sucrose synthase showed the higher activity during November to February, and almost low activity during all the experimental periods. The sucrose phosphate synthase (EC 2.4.1.14) also showed higher activity during October to February, but the activity levels did not change drastically throughout the fruit development. The results substantiate the conclusion that, in all the planting seasons, acid invertase is a principal enzyme in the process of tomato fruit ripening and during early stage of tomato fruit development, sucrose synthase is the dominant enzyme, which, in turn, plays a part in regulating the translocation of sucrose into the fruit.
A Student-driven Landscape Design for a New Academic Building
Authors: Stevenson Elizabeth A. , Grueber Kevin L. 
Published: February 2019

Berry College offers a unique environment for learning with 28,000 acres of forests, meadows, lakes, and streams. This distinctly beautiful setting has encouraged environmental awareness among students and faculty on campus. The construction of an academic building to house the School of Math and Natural Sciences in a previously undisturbed, wooded site prompted students and faculty to become interested in the preservation of the site's natural characteristics. Students in the horticulture program worked closely with the Director of Horticulture and the Academic Dean to develop a plan to create a landscape that was both educationally and environmentally sound.
The plan consisted of a detailed landscape design as well as the identification of the steps necessary to implement the design. The design incorporated ornamental plants and geological features native to the southeastern region of the United States with the plant species that existed on the site. The design contains such features as a wildflower meadow, an aquatic garden, rock gardens, and various native trees, shrubs, and groundcovers. Plant materials were properly labeled and brochures are made available to guests, students, and faculty interested in learning more about indigenous geological features and plant materials while touring the building and its landscape. The success of this project is due to the cooperation and participation of faculty, staff, and students and represents a unique learning opportunity.
(87) Short-term Freeze Stress Attenuation in St. Augustine Turfgrass with Selected Biostimulants
Authors: Morales-Payan J. Pablo , Stall William M. 
Published: February 2019

Experiments were conducted to determine the effects of selected biostimulants on St. Augustine turfgrass exposed to short-term periods of freezing temperatures, which are common in north-central Florida during March and April. Aqueous solutions of a triterpenic acid-rich extract from Siberian fir (Abiessibirica) [(TTA), 0 and 300 mg·L-1 a.i.], a seaweed (Ascophyllum nodosum) extract [(CSE), 30 mg·L-1 a.i.], acetylthioproline [(AP), 250 mg·L-1 a.i.], and amino levulinic acid [(5-ALA), 15 mg·L-1 a.i.] were sprayed on residential St. Augustine turfgrass about 50 hours prior to the forecasted freezing event. After freezing, the aesthetic quality of AP-treated St. Augustine turfgrass was the same as in untreated turfgrass plots, but it was drastically reduced in turfgrass treated with 5-ALA. In contrast, St. Augustine tufgrass aesthetic quality was higher in CSE- and TTA-treated plots than in untreated plots. These results indicate that CSE and TTA may help alleviate the negative effects of short-term exposure to freezing temperatures in St. Augustine turfgrass.
(131) Nitrogen and Potassium Changes during Growth of Poinsettia (Euphorbia pulcherrimaWild) `Supjibi Red'
Authors: Rosas-Ramos Jorge-Moises , Castillo-Gonzalez Ana-Maria , Colinas-Leon María Teresa , Pineda-Pineda Joel 
Published: February 2019

Nitrogen and potassium changes in poinsettia `Supjibi Red' were evaluated at four stages: vegetative growth, inductive stage, bract development and flowering. Nitrogen doses were 100, 200, 300, 400, and 500 mg·L-1 and those of potassium were: 175, 250, 325, and 400 mg·L-1, which were applied constantly with irrigation. Plants were grown in 7-inch pots with tezontle as the substrate. A completly random experimental design with nine treatments and 10 replicates was used. Short photoperiod was applied from 61 days after transplant, covering plants for 14 hours from 7 pm on. Samples for nitrogen and potassium evaluations were taken from recently grown-up leaves at 25, 50, 75, 100 and 125 days after transplant. Nitrogen absorption increased up to 75 days after transplant; treatments with 400 and 500 mg·L-1 of nitrogen induced the highest foliar levels 2.44% and 2.6%, respectively. In the following sampling dates, nitrogen decreased for all treatments. Potassium content decreased as plants developed; highest levels were obtained 25 days after transplant. The 325 and 400 mg·L-1 K treatments induced the highest concentrations in leaves 7.04% and 7.02%, respectively. Thus, it is confirmed that nitrogen is required throughout vegetative growth, and potassium just in the initial stage of vegetative growth.
Repetition as the Performative Syndrome of Dying
Authors: Chukhrov Keti 
Published: February 2019

In his Difference and Repetition Deleuze reveals an aporia: repetition is singular, solitary, it is torn away from any original or source; nevertheless it preserves a genetic tie with certain event to which it is a repetition. This solitariness of the repetition is not, however, confined to mere difference between the act of repetition and the repeated source that cancels the original just to differentiate two performative procedures. An act of repetition is solitary only when it evolves in specific time-regime, which even ontically diverges from the regular ontology of time. Deleuze calls such temporality “empty”, Nietzsche defines it as amor fati, Heidegger sees in it convergence of eternity and an instant. The stake in this case is a specific kind of repetitive regime which unfolds as the performative syndrome of ‘dying’ – a “repetition into death” (Deleuze) which paradoxically executes itself as performative plenitude. But who is the Subject undergoing such a syndrome and what should have happened to her/him so as to impose the regime of dying on any act of repetition?
A stormwater user fee model for operations and maintenance in small cities
Authors: Tasca Fabiane Andressa , Finotti Alexandra Rodrigues , Goerl Roberto Fabris 
Published: January 2019

Abstract
In this paper, a stormwater fee specifically for small municipalities was developed through a simplification of the Equivalent Residential Unit (ERU) system, which is the main method applied in the United States. The Simplified ERU is based on the amount of impervious area and the fee considers the operations and maintenance costs, besides having a single class of billing. It was applied in Santo Amaro da Imperatriz city, located in the southern region of Brazil, which can be classified as a small city. The value of the Simplified ERU (294.32 m2) was similar to the average impervious area in the United States (269.42 m2) and it was equivalent to $0.28 per square meter (within the range of other countries' fees). The method proved to be a feasible and rapid technique for funding stormwater services, and its simplicity allows its application in different locations. This funding model can generate revenue to address current stormwater problems, such as the lack of funding for existing drainage infrastructure, and contribute to downstream flooding reduction. This paper also intends to encourage discussion about the methods for designing a stormwater fee in the academic community, which is still incipient.
Preparation of graphene oxide–montmorillonite nanocomposite and its application in multiple-pollutants removal from aqueous solutions
Authors: Zhang Chengyu , Luan Jingde , Chen Wei , Ke Xin , Zhang Haijun 
Published: January 2019

AbstractIt is of interest to develop a novel fabrication method of a mineral adsorbent for wastewater treatment to remove the combination of heavy metal ions and refractory organic contaminants. The crosslinking agent stearyl trimethyl ammonium chloride was added into a suspension of montmorillonite and graphene oxide to implement their recombination to fabricated graphene oxide–montmorillonite nanocomposite (GMN). The fabricated nanocomposite was characterized by X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller analysis and zeta potential. Results indicated that GMN exhibited a honeycomb texture, providing the chemical reaction site for the simultaneous adsorption of Pb2+ and p-nitrophenol (PNP). Factors including pH value, contact time, contact temperature and GMN dosage in the adsorption process were crucial for both Pb2+ adsorption and PNP adsorption. The optimum adsorption capacities of Pb2+ and PNP onto GMN were 19.39 mg·g−1 and 14.90 mg·g−1 under the condition of pH = 6, contact temperature 55 °C, contact time 60 min and GMN dosage 0.10 g, respectively. Data from experimental studies on simultaneous adsorption was well described by the pseudo-second-order model. The implementation of this work shows that GMN is a promising material for application in the simultaneous removal of heavy metal ions and refractory organic contaminants.
Natural Products and their (Semi-)Synthetic Forms in the Treatment of Migraine: History and Current Status
Authors: Tauchen Jan 
Published: January 2019

Background:
Migraine may be described as a headache with moderate to extreme pain
that is often accompanied by incapacitating neurological symptoms. It is estimated that 12% of the
world population suffers from migraine. Although a number of drugs have been used for treatment
of migraine, most of these are not effective for every patient and may have undesirable side-effects.
Thus, there is an enormous unmet need in current migraine therapy for discovering safer and more
effective agents.

Methods:
The information summarized in this review was obtained through extensive literature
review and search of relevant books and articles with the use of Web of Knowledge and SciVerse
Scopus databases.

Results:
Greater understanding of the molecular mechanisms underlying the etiopathogenesis of
migraine is helpful in identifying novel targets for antimigraine drugs such as cannabinoid, histamine,
and melatonin receptors. In the past, natural product-derived constituents have served as an
invaluable source of numerous medicinally useful antimigraine agents and it may be expected that
further promising drug candidates from natural products will be discovered for antimigraine pharmacotherapy
with better efficacy and fewer adverse-effects.

Conclusion:
The discovery of novel targets in migraine therapy has opened new horizons for compounds
that have not been clinically tested or that previously failed in clinical trials as potential antimigraine
drugs. Ginkgolide B, melatonin, histamine, oxytocin, various ribosomal peptide toxins,
kavalactones, devil’s claw-derived compounds, salvinorin A and petasin are among those agents that
show considerable promise as novel drugs in migraine prevention and treatment. It is necessary to
conduct more research to better understand their antimigraine action, to confirm their effectiveness
and safety, and to introduce them into clinical practice.
Efficacy and Safety of Addition of Anti-PD1 to Chemotherapy in Treatment of Non-Small Cell Lung Cancer
Authors: Ru Chu-Hui , Zhuang Yan-Bing 
Published: January 2019

Background:
Patients with previously treated non-small-cell lung cancer (NSCLC) have
limited treatment options. A novel treatment based on programmed death 1 (PD-1)/programed death
ligand 1 (PD-L1) inhibitors has emerged as promising therapeutic options for advanced NSCLC. We
assessed oncological outcomes of PD-L1 antibody versus docetaxel in previously treated NSCLC.

Objectives:
The purpose of this meta-analysis was to analyse the oncological outcomes of anti-PD1
to chemotherapy in the treatment of non-small-cell lung cancer.

Results:
Overall survival (OR=0.68,95%CI=0.61-0.75, P<0.00001) and progression-free survival
(OR=0.84,95%CI=0.77-0.92, P=0.0002) were longer with anti-PD1 than with docetaxel in NSCLC.
Anti-PD1 was associated with even greater objective response rate than docetaxel
(OR=1.61,95%CI=1.16-2.24, P=0.004). Treatment-related adverse events of grade 3-5 did favor
anti-PD1 over docetaxel (OR=0.21,95%CI=0.10-0.42, P<0.00001).

Conclusions:
Among patients with advanced NSCLC, we found that there was a superior survival
benefit and with a favorable safety profile with anti-PD1 than with docetaxel. More large-scale
randomized controlled trials are needed to identify relevant biomarkers that have an effect on
predicting the population that would most likely benefit from PD-1/PD-L1 for pretreated advanced
NSCLC patients.
Synthesis of Some Novel Quinolinols with In-vitro Antimicrobial, and Antioxidant Activity
Authors: Shehab Marwa A. Sh. , El-Naggar Mohamed , Ismail Rabab A. , El Kafrawy Hala M. , Abood Amira , Ismail Shaymaa A. , Sabry Nermien M. , El Sayed Mardia T. 
Published: February 2019

Background:
Amongst the quinolone core structures, 8-hydroxyquinoline (8-HQ or quinolinol)
stands out as the greatest frequently used therapeutic moietiy. This includes the most critical molecules
in medicinal chemistry. Quinolinol remains a broad-spectrum ligand capable of chelating to a
large number of metal ions.

Methods:
The synthesized quinolinols Mannich bases were screened for their in vitro antimicrobial activity
against Staphylococcus aureus (ATTCC 6538), Escherichia coli (ATTCC 7839), Klebsiella
pneumonia (ATCC10131). The antifungal activity of the prepared compounds was assessed against
Candida albicans (10231), Aspergillus niger and Penicillium sp. The antioxidant activity of the
established compounds was assessed by means of α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical
scavenging method.

Results:
The antimicrobial outcomes indicated that all the synthesized compounds excluding 5 and 9b
displayed reasonable antibacterial activity against Staphylococcus aureus (ATTCC 6538) and
Escherichia coli (ATTCC 7839) with an inhibition zones ranging from 13 to 23 mm. However, in the
case of Klebsiella pneumonia (ATCC10131) only compound 6 did not show any activity. The results
also indicated that compounds 2b and 3 were the most potent antibacterial compounds against the
verified strains with minimum inhibitory concentration (MIC) values ranging from 0.05 to 0.5mg/ml. In
the antifungal assay, all compounds showed good activity against Candida albicans (10231) except
compounds 5 and 9b. However, in the case of Aspergillus niger and Penicillium sp. only compounds
2b and 3 showed good activity. In the antifungal assay, MIC values for compounds 2b and 3 ranged
from 0.25 to 2.5 mg/ml against the specified fungal strains. The antioxidant activity was assessed using
the DPPH scavenging activity method. The results indicated that 2b was the most active among all
tested compounds, with almost double the antioxidant activity as compared with that of trolox (positive
control).

Conclusion:
In this work, we describe the synthesis of new Mannich bases comprising 8-HQ (1) and its
derivative (8). The resulted Mannich bases of type 2 were used in transamination reactions with hydrazine
and hydrazine derivatives. The structures of the newly synthesized Mannich bases were confirmed
based on the NMR spectroscopic data and elemental analysis. Antimicrobial and antioxidant activities
were also assessed.