bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chloroplast genome analysis and genetic transformation of Parachlorella kessleri-I a potential marine alga for biofuel production
Authors: Nawkarkar Prachi , Chugh Sagrika , Tanwar Amita , Sharma Surbhi , Kaushal Charli , Abdin Malik Zainul , Jain Mukesh , Kumar Shashi 
Published: July 2019

Abstract
Background: Parachlorella kessleri-I produces higher biomass and lipid content suitable for commercial production of biofuels. Sequencing complete chloroplast genome will be instrumental in the constructing species specific chloroplast transformation vectors and generating chloroplast transgenic microalga with the desired traits and greater productivity, essential for commercial sustainability of microalgae based biofuel production.

Results: Complete chloroplast genome sequence (cpDNA) of P. kessleri-I was annotated. The 109,642 bp chloroplast genome exhibited a quadripartite structure with two reverse repeat regions (IRA and IRB), a long single copy (LSC) and a small single copy (SSC) region. The genome encodes 117 unique genes, with 70 predicted protein coding genes, 35 tRNAs, 4 rRNAs. The cpDNA provided essential information like codons, UTRs and flank sequences for homologous recombination to make a species specific chloroplast transformation vector that facilitated chloroplast transformation of P. kessleri-I. To optimize chloroplast transformation, two antibiotic resistance makers aminoglycoside adenine transferase (aadA) conferring resistance to spectinomycin and Sh-ble gene from bacteria that conferred resistance to zeocin were tested. Using a aadA gene, transgenic colonies were retrieved on TAP medium containing 400 mg/l spectinomycin. However, no transgenic colonies were recovered in the zeocin supplemented medium. The spectinomycin resistant algal cell lines were analyzed by PCR. Southern blotting confirmed the stable transgenes integration into the chloroplast genome of P. kessleri-I via homologous recombination.

Conclusion: The complete chloroplast genome analysis may provide valuable resources for population and evolutionary studies of Parachlorella species and identifying the related species. The chloroplast genome of P. kessleri-I was assembled as a quadripartite structure of 109,642 bp with defined IR regions. Its complete sequencing has provided essential information like codons, UTRs and flanking sequences to generate the species specific chloroplast transformation vector and obtaining the successful site-specific chloroplast transformation in P. kessleri-I via homologous recombination. The optimized chloroplast transformation in marine alga P. kessleri-I should open a new possibilities like controlling the chain lengths of fatty acids for biofuel application, manipulating the RubisCo enzyme for improving photosynthetic process, introducing carbon concentration mechanism (CCM) for fixing higher CO2 that may be instrumental in producing economically viable biofuel molecules.
Meta-analysis of the efficacy of pulmonary surfactants combined with budesonide for the prevention of bronchopulmonary dysplasia
Authors: He Bingbin , Zhuang Jiayong , Ye Boran , Liu Guosheng 
Published: July 2019

Abstract
Background

Pulmonary surfactants(PS) combined with the intratracheal instillation of budesonide to prevent bronchopulmonary dysplasia (BPD) have been report previously. However, the safety of the use of PS combined with budesonide is still unknown and remains to be clarified.

Methods

PubMed, the CochraneLibrary, EMBASE, the China Knowledge Network and the Wanfang database were searched for relevant studies. Searches were performed from December 2018, and data from randomized controlled trials (RCTs) were collected. Primary outcome measures were BPD incidence, BPD-related mortality. Secondary outcomes were BPD-related complications. The Cochrane risk assessment tool was used for the evaluation of bias. RevMan5. 3software was employed for meta-analysis. An Egger's test was used for publication bias assessments.

Results

A total of 720 subjects were enrolled from 6 RCTs, including 352 in the experimental group and 368 in the observation group. A meta-analysis showed that the incidence of BPD [RR=0. 42, 95% CI (0. 37, 0. 89), P < 0.001] and BPD-related mortality [RR = 0.54, 95% CI (0.38, 0.89), P<0.05] significantly differed between the groups. Differences were also observed for intraventricular hemorrhage, infection/sepsis, retinopathy of prematurity (ROP), and patent ductus arteriosus. There were no significant differences in the incidence of PDA, neonatal necrotizing enterocolitis (NEC), hyperglycemia, or hypertension (P > 0.05).

Conclusion

The intratracheal instillation of pulmonary surfactants with budesonide can reduce the incidence of BPD and BPD-related mortality, with no increased risk of short-term complications. However, considering both sample size and study bias, the safety and efficacy of this treatment plan requires clarification in large-samples, and multi-center clinical RCTs. In addition, the impact of long-term complications such as neurodevelopmental disorders requires further assessment.
De la psicoterapia a la teoterapia. Sentidos subjetivos respecto al proceso teoterapéutico en personas adictas a sustancias psicoactivas con experiencia previa en psicoterapia
Authors: Kameniecki Mario 
Published: July 2019

Posiblemente las llamadas adicciones a las drogas son uno de los temas que más polémicas suscita en la sociedad; despierta desde las condenas más radicales hasta, en el otro extremo, posiciones de absoluta liviandad. Hay que decir que el uso de drogas forma parte de algo más vasto: el fenómeno de las drogas, con múltiples aristas entre las cuales el consumo –cualquiera sea su modalidad- es el último eslabón de una compleja cadena de elementos. Se caracteriza en nuestro tiempo por el uso masivo de sustancias psicoactivas (SPA). Es lo que condujo a Alain Ehrenberg hace algunos años a considerar el consumo de nuestra época como una toxicomanía de masas. También es oportuno decir que el fenómeno de las drogas es un constructo social armado principalmente por los medios de comunicación como algo homogéneo y uniforme. Es el consumo de drogas y en especial las adicciones, es decir, los estados de dependencia a ellas, los que se articulan con sus posibles tratamientos.
A IDEIA DE LIBERDADE EM KANT
Authors: Pimentel JÃºlia Mara Rodrigues , Arakaki Fernanda Franklin Seixas 
Published: July 2019

Este artigo tem por escopo analisar o conceito de liberdade desenvolvido pelo filÃ³sofo alemÃ£o Immanuel Kant â€“ considerado por muitos como o pai da filosofia moderna â€“ como um dos elementos centrais da ideia de justiÃ§a, ao lado da igualdade. Para tanto, trabalhar-se-Ã¡, sob uma perspectiva crÃ­tica, tanto a ideia de liberdade como a noÃ§Ã£o de igualdade, ambas acasteladas por Kant. Ligadas Ã ideia de justiÃ§a, indesatÃ¡veis estÃ£o as ideias de liberdade e de igualdade. Destarte, para o filÃ³sofo, o exercÃ­cio da liberdadeÂ Â de cada um deve compatibilizar-se com o da liberdade de todos os demais segundo um princÃ­pio de igualdade.
O USO DA HISTÃ“RIA ORAL: A HISTÃ“RIA PRODUZIDA NA POSTERIORIDADE
Authors: Hot Amanda Dutra , Campos Germano Moreira , Soares Juliana Souza Martins , Alves Leonardo de Carvalho , Correa Leonardo Souza 
Published: July 2019

Este trabalho explicarÃ¡ os usos e os problemas da HistÃ³ria Oral (H.O), uma nova metodologia da histÃ³ria surgida no sÃ©culo XX, mais precisamente em1950, apartir do surgimento do gravador. Tal mÃ©todo baseia-se na realizaÃ§Ã£o de entrevistas direcionadas pelo historiador Ã s testemunhas que vivenciaram, mesmo como simples espectadores, determinado evento, o que faz da H.O uma metodologia restrita ao perÃ­odo contemporÃ¢neo e que trabalha com oÂ â€œconhecimento da posterioridadeâ€, ou seja, com informaÃ§Ãµes obtidas apÃ³s a ocorrÃªncia do evento, nÃ£o deixando, por isso, no entanto, de ter suas utilidades, afinal de contas os relatos das testemunhas servem e muito para ajudar aos historiadores a preencherem as lacunas existentes na historiografia.
REPOSICIONAMENTO DA EMPRESA FAMILIAR EM SEU PROCESSO SUCESSÃ“RIO: UM ESTUDO DE CASO DAS LOJAS NG - MANHUAÃ‡U (MG)
Authors: Vieira Neidiane Pimentel Breder , Souza Reginaldo Adriano de , Alvim-Hannas Anandy Kassis de F. , Souza JosÃ© Carlos de 
Published: July 2019

O processo de reposicionamento das empresas Ã© fator essencial para estas se adequarem Ã s mudanÃ§as ocorridas no ambiente. Este artigo tem como principal objetivo identificar os fatores contingenciais que impulsionaram a Loja NG a se reposicionar no mercado apÃ³s a sucessÃ£o familiar ocorrida e ainda verificar a percepÃ§Ã£o dos clientes quanto Ã s mudanÃ§as ocorridas nesse processo. A metodologia utilizada teve como base o estudo de caso e pesquisa descritiva. Para o levantamento dos dados foram utilizadas as anÃ¡lises quantitativas e qualitativas, com o intuito de buscar dados da percepÃ§Ã£o dos clientes e anÃ¡lise do gestor da empresa, durante o reposicionamento. Os dados obtidos foram favorÃ¡veis Ã s mudanÃ§as ocorridas, os clientes percebem, de forma clara, os objetivos e aÃ§Ãµes propostas pela empresa neste novo posicionamento adotado. Assim, a organizaÃ§Ã£o busca a melhoria contÃ­nua da qualidade dos serviÃ§os oferecidos, visando ao bem- estar e Ã satisfaÃ§Ã£o dos clientes.
Prediction of dissolved oxygen in urban rivers at the Three Gorges Reservoir, China: extreme learning machines (ELM) versus artificial neural network (ANN)
Authors: Zhu Senlin , Heddam Salim 
Published: July 2019

AbstractIn the present study, two non-linear mathematical modelling approaches, namely, extreme learning machine (ELM) and multilayer perceptron neural network (MLPNN) were developed to predict daily dissolved oxygen (DO) concentrations. Water quality data from four urban rivers in the backwater zone of the Three Gorges Reservoir, China were used. The water quality data selected consisted of daily observed water temperature, pH, permanganate index, ammonia nitrogen, electrical conductivity, chemical oxygen demand, total nitrogen, total phosphorus and DO. The accuracy of the ELM model was compared with the standard MLPNN using several error statistics such as root mean squared error, mean absolute error, the coefficient of correlation and the Willmott index of agreement. Results showed that the ELM and MLPNN models perform well for the Wubu River, acceptably for the Yipin River and moderately for the Huaxi River, while poor model performance was obtained at the Tributary of Huaxi River. Model performance is negatively correlated with pollution level in each river. The MLPNN model slightly outperforms the ELM model in DO prediction. Overall, it can be concluded that MLPNN and ELM models can be applied for DO prediction in low-impacted rivers, while they may not be appropriate for DO modelling for highly polluted rivers.This article has been made Open Access thanks to the kind support of CAWQ/ACQE (https://www.cawq.ca).
Principal component analysis for decision support in integrated water management
Authors: Helness H. , Damman S. , Sivertsen E. , Ugarelli R. 
Published: July 2019

Abstract
A general methodology for holistic sustainability assessment of measures in integrated water management based on principal component analysis (PCA) was developed. Application on data from three cases demonstrated that PCA could be used to rank alternatives, assess differences between groups of alternatives and the main properties responsible for this, and account for the impacts of measures on different dimensions of sustainability. The results demonstrated the general applicability of the method. For all cases a combination of measures/options yielded the most sustainable solution. The absence of a single clearly most optimal solution highlights the need for a transparent and systematic analysis, which can be obtained with the presented methodology.
Effects of Dietary Management Education on Self-Efficacy and Caregiver Practice in Dietary Care of Family Members with Type 2 DM
Authors: Anggraini Ria , Suryawati Chriswardani , Rachma Nurullya 
Published: July 2019

<p><em>Type 2 Diabetes Mellitus </em><em>(DM) </em><em>is one of the public health problems with high prevalence </em><em>both </em><em>in Indonesia and in the word. According to the World Health Organization (2016), 90% of all DM cases around the world is of type 2 DM. </em><em>D</em><em>ata from the Indonesian Ministry of Health in 2014 also showed that 90% of all diabetic cases in Indonesia w</em><em>ere</em><em> of type 2 DM. In caring for the family members with type 2 DM</em><em>, c</em><em>aregivers require self-efficacy and practice of </em><em>dietary </em><em>care. This self-efficacy and </em><em>care </em><em>practice can be improved through dietary management education. This study aimed to analyze the effects of type 2 DM dietary management education on self-efficacy and </em><em>dietary </em><em>care practice of caregivers. This</em><em> quantitative </em><em>study </em><em>used</em><em> a pre and posttest quasi-experiment</em><em>al design </em><em>with a control group</em><em>. </em><em>The samples were 44 caregivers recruited using purposive sampling technique and were assigned to two groups based on the inclusion and exclusion criteria. The results </em><em>showed that </em><em>dietary management education </em><em>could </em><em>improve self-efficacy and </em><em>caregiver practice in the d</em><em>ietary care </em><em>of </em><em>family members with type 2 DM (p-value=0.000 and α=0.05). This study concluded that type 2 DM dietary management education program gave effects on self-efficacy and caregiver</em><em> </em><em>practice. Dietary management education can be used as an </em><em>alternative </em><em>to improve self-efficacy and caregiver</em><em>s’ </em><em>practice</em><em> in the dietary care of patients with type 2 DM</em><em>.</em><em></em></p>
Premenstrual Dysphoric Disorder Causes Discomfort And Interfere Adolescent’s Social Relationship
Authors: Marfuah Dewi 
Published: July 2019

<pre><em>Menstruation is the discharge of blood from the uterus through the vagina which happens monthly</em><em>. </em><em>Premenstrual syndrome (PMS) is </em><em>one of menstrual disorder and </em><em>a health problem in reproductive women with prevalence about 20-40%. While, P</em><em>remenstrual Dysphoric Disorder (P</em><em>MDD</em><em>)</em><em> is severe form of PMS with prevalence about 3-8%. Prevalence of PMS and PMDD increased in Pasca of disaster area about 47,3% and 41,8%. However, no researches aim to describe about life experience of adolescence with PMDD’s syndromes in pasca of disaster area. </em><em>This study aimed </em><em>to describe adolescents’s experience who experiencing symptoms of PMDD.</em><em> This study used a</em><em> qualitative study </em><em>with</em><em> phenomenological approach. Number of participants were six female students of SMK Negeri 1 Cangkringan</em><em>, Yogyakarta, Indonesia</em><em> that meet PMDD based on </em><em>the </em><em>Diagnostic</em><em> </em><em>and Statistic Manual of Mental Disorders</em><em> </em><em>DSM-IV. In-depth interviews with a unstructured interview and using Colaizzi method</em><em> for analysis data</em><em>.</em><em> Thus </em><em>for trustworthiness with data source triangulation</em><em> to their teachers and classmates. The results found four</em><em> main themes describing the experience of adolescence with PMDD’s syndromes were formed: symptoms perceived as a change that affects the psychological, behavioral and physical; symptoms are intermittent along the menstrual cycle; environmental and hormonal factors play a role in the onset of symptoms; </em><em>and </em><em>symptoms cause discomfort and interfere social relationships of adolescents</em><em>. </em><em>Adolescents's experience with symptoms of PMDD post-disaster as like as the normal condition that cause discomfort that interferes adolescent’s social relationship. Nurses and other media personnel in the health care institution is expected to more actively participate to provide a wide range of related reproductive health education</em><em> and giving intervention to reduce PMDD in order to increasing Women’s Quality of Life.</em></pre>
ANALISIS KERENTANAN SISTEM IRIGASI AKIBAT PERUBAHAN IKLIM DENGAN TOLOK UKUR INDEKS KELENTINGAN DI EMPAT DAERAH IRIGASI WILAYAH DAERAH ALIRAN SUNGAI BRANTAS
Authors: Lestari Wahyuni Setyo , Suprayogo Didik , Prijono Sugeng 
Published: July 2019

Climate change which mainly affected by the increase of climate variability from time to time is highly impacted to the vulnerability of irrigation system, in a way that by the increase of water demand can not be fulfilled by water availability (the decrease of capacity). While, basic measure of conducting irrigation system management currently is solely based on comparison value between demand and irrigation water availability (K-factor) without considering the increase of climate variability. Resilience Index (Ik) as one of basic measures in water resource management can be used as a limitation in irrigation system operational management. The results of the study show that Molek, Jatikulon, Menturus and Konto irrigation areas (DI) are impacted by climate change with different scales of resilience. The resilience index of 100% for Molek Irrigation area, 80% for Jatikulon irrigation area, 50% for Menturus irrigation area, and 33% for Konto irrigation area. Climate change is predicted causing the decrease of harvested area, in such a way that in the condition of El Niño around 48% in Konto Irrigation Area, 34% in the Menturus Irrigation Area, 28% at the Jatikulon Irrigation Area, and 15% in the Molek Irrigation areas. Nevertheless, in the La-Nina condition, there was no the increase of harvested area in the entire of irrigation area studied, even, there was a decreased of harvested area of 28% in Konto irrigation area, and 6% in Menturus irrigation area. The most powerful threat toward irrigation system sustainability is the environment, followed by physical, social, and economic aspects respectively. The results of this study implicate to the imprevement of irrigation management policy, namely to the operational pattern of irrigation system, which use resilience index (Ik) as basic measure of irrigation system management out of the K-factor.
Risk Mitigation on Supply Chain of Rice in Central Java Province and Special Region of Yogyakarta
Authors: Guritno Adi Djoko , Kristanti Novita Erma , Tanuputri Megita Ryanjani 
Published: July 2019

Rice is a vital commodity in Indonesia because it is the main staple food for most Indonesian people. Balancing supply and demand of rice in Indonesia is complex and possibly led to polemics, even though the production of rice in Indonesia tends to increase. An analysis of the supply-demand for rice commodity is necessary as an effort to improve the national rice condition along with the logistics and supply chain point of view. This study aims to analyze the supply chain risk management of rice to provide recommendations on improving rice supply chain performance on each tier. In this study, supply chain of rice mainly consists of farmer, collector who acts as trader, and retailer. Meanwhile, the role of government is represented by The Indonesia Logistics Bureau (BULOG). The different supply chain pattern can be identified from the role of middleman in each province. This study uses ISO 31000:2009 standard for analyzing the risk in each tier along the supply chain. Based on the risk analysis, on-farm activity plays a vital role in transferring risk along the supply chain in terms of quality and quantity. Risk mitigation of each tier is explored in this study.
Centroamérica en una nuez. Vuskovíc Céspedes, Pedro, Centroamérica: fisonomía de una región, México, PECA-CIDE, 1987, 107 pp.
Authors: León José Luis 
Published: July 2019

No por casualidad, los estudios internacionales y latinoamericanos en la década de los ochenta se han caracterizado por una noble proliferación de asuntos conectados con el acontecer de Centroamérica.
Hacia la equidad: la llegada de las mujeres como estudiantes universitarias
Authors: Zuleta Medina Alejandra 
Published: July 2019

Este artículo se ubica en la categoría denominada investigación; en él se expone, en términos generales, el ingreso de las estudiantes a la universidad, así como los obstáculos que debieron sortearse y algunos resultados favorables de su lucha. A partir del siglo XX, la participación femenina en todas las esferas sociales y públicas es activa e indiscutible a nivel mundial. De esta manera, se cree que los prejuicios contra la mujer prácticamente han desaparecido. No obstante, se han presentado diferencias significativas con las circunstancias, condiciones y oportunidades que rodean el actuar femenino y masculino, incluso en espacios de transformación social y discusión científica, como las instituciones de Educación Superior. En este texto, se abordarán algunas de las dificultades superadas por las pioneras universitarias en Europa, Estados Unidos y América Latina a nivel general y, de forma más específica, en Colombia y la Universidad de Nariño.
Meningkatkan Keterampilan Gerak Tari Melalui Strategi Praktik Berpasangan Kelas IV SD Negeri 66/IV Kota Jambi
Authors: Destrinelli D , Leony L 
Published: July 2019

Abstract. This study aims to describe the application of paired practice strategies (Practice Rehearsal Pairs) to improve dance movement skills of students in 4th grade elementary school dance learning. This research was conducted in 66 / IV Public Elementary School in Jambi City in the even semester of the 2018/2019 academic year. This type of research is classroom action research, which consists of II cycles with procedures starting from planning, implementation, observation, and reflection. The subjects of this study were grade IVB students of SD 66 / IV in Jambi City. Instrument Data collection used was teacher action observation and practice test (performance) of student dance movement skills. Data were analyzed qualitatively and quantitatively. The results of the percentage of student practice tests (performance) amounted to 45% with less categories in the first cycle and increased in the second cycle by 82.5% in the good category. From the results of this study, it was concluded that by implementing a paired practice strategy (Practice Rehearsal Pairs) can improve students' dance skills in fourth grade elementary school dance learning.

Keywords: Practice Rehearsal Pairs, dance movements skills
The Inclusive Education Program in Jambi: Voices from Insiders
Authors: ROSMIATI ROSMIATI , GHAFAR ABDOEL , TABRONI TABRONI , RAHMAN ADITYA 
Published: July 2019

This study was aimed at exploring the inclusive education programs developed by the government including inclusion-education curriculum, special mentor recruitment, and infrastructure facilities in the schools. The study used a qualitative method with a case-study tradition. The data were collected through interviews with 8 informants, 5 classroom observations, and documents at one elementary school. The interview data were analyzed using within-case and cross-case analyses among informants. The observational data were recorded and presented in accordance with the themes of the interview results and document data were used as the comparison. The results revealed that there were no new student admission preparation, and lack of government attention on infrastructure. The presentation of the data also included the challenges faced in implementing inclusive education. Suggestions and implications for schools in the implementation of inclusive education and the government are discussed.