bluuebunny/crossref_metadata_2025_split huggingface dataset:
Campylobacter fetus is Internalized by Bovine Endometrial Epithelial Cells
Authors: CAMPOS-MÚZQUIZ LIZETH GUADALUPE , MÉNDEZ-OLVERA ESTELA TERESITA , ARELLANO-REYNOSO BEATRIZ , MARTÍNEZ-GÓMEZ DANIEL 
Published: June 2019

Abstract

Campylobacter fetus is an important venereal pathogen of cattle that causes infertility and abortions. It is transmitted during mating, and it travels from the vagina to the uterus; therefore, an important cell type that interacts with C. fetus are endometrial epithelial cells. Several virulence factors have been identified in the genome of C. fetus, such as adhesins, secretion systems, and antiphagocytic layers, but their expression is unknown. The ability of C. fetus to invade human epithelial cells has been demonstrated, but the ability of this microorganism to infect bovine endometrial epithelial cells has not been demonstrated. Bovine endometrial epithelial cells were isolated and challenged with C. fetus. The presence of C. fetus inside the endometrial epithelial cells was confirmed by the confocal immunofluorescence. C. fetus was not internalized when actin polymerization was disturbed, suggesting cytoskeleton participation in an internalization mechanism. To evaluate the intracellular survival of C. fetus, a gentamicin protection assay was performed. Although C. fetus was able to invade epithelial cells, the results showed that it did not have the capacity to survive in the intracellular environment. This study reports for the first time, the ability of C. fetus to invade bovine endometrial epithelial cells, and actin participation in this phenomenon.
Bacterial Diversity in Soybean Rhizosphere Soil at Seedling and Mature Stages
Authors: WANG LIN , LI ZHIYING , LIU RUIRUI , LI LULU , WANG WEIWEI 
Published: June 2019

Abstract
Changes in the structural diversity of bacterial communities in soybean rhizospheres play important roles in plant growth and crop productivity. However, there are only a few studies on different soybean growth stages. Here, we investigated the changes in the bacterial community of soybean rhizosphere soil at two stages using Illumina high-throughput sequencing. The results showed that the bacterial abundance and diversity in the seeding stage were higher than those in the mature stage and that the diversity changed significantly. Actinobacteria, Acidobacteria, and Proteobacteria were the dominant bacteria in the soybean rhizosphere soil. Additionally, changes in Actinobacteria and Proteobacteria abundances showed opposite trends.
Patterns of Drug-Resistant Bacteria in a General Hospital, China, 2011–2016
Authors: MAO TINGTING , ZHAI HUIJUAN , DUAN GUANGCAI , YANG HAIYAN 
Published: June 2019

Abstract
Drug-resistant bacteria has been a threat to public life and property. We described the trends and changes in antibiotic resistance of important pathogens in a general hospital in Zhengzhou, China from 2011 to 2016, to control antimicrobial-resistant bacteria in hospital and provide support to clinicians and decision-making departments.
Five dominant bacteria were enrolled based on the data from the general hospital during 6 years. The results of antimicrobial susceptibility testing were interpreted according to Clinical and Laboratory Standards Institute (CLSI). From 2011 to 2016, a total of 19,260 strains of bacteria were isolated, of which Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii accounted for 51.98%. The resistance rate of K. pneumoniae and E. coli to carbapenem was less than 15%, but resistance of K. pneumoniae to carbapenems increased with time and resistance of E. coli to meropenem increased. The rate of extended-spectrum beta-lactamase (ESBL) production among K. pneumoniae and E. coli was decreasing. For most antibiotics, the resistance rate of ESBL-positive isolates was higher than that of ESBL-negative isolates, excluding carbapenems and cefoxitin. For S. aureus, the rate of methicillin-resistant S. aureus (MRSA) was stable. Resistance of S. aureus to mostly antibiotics decreased with time. Besides polymyxin B, P. aeruginosa and A. baumannii showed high resistance to other antibiotics. For A. baumannii, the resistance rate to mostly antibiotics was increasing. The bacteria showed high levels of resistance and multiple drug resistance. Continuous surveillance and optimizing the use of antibiotics are essential.
Gastric Microbiota Alteration in Klebsiella pneumoniae-Caused Liver Abscesses Mice
Authors: CHEN NAN , JIN TONG-TONG , LIU WEN-NING , ZHU DONG-QING , CHEN YING-YING , SHEN YUE-LIANG , LING ZONG-XIN , WANG HONG-JIE , ZHANG LI-PING 
Published: June 2019

Abstract
Gastric microbiota provides a biological barrier against the invasion of foreign pathogens from the oral cavity, playing a vital role in maintaining gastrointestinal health. Klebsiella spp. of oral origin causes various infections not only in gastrointestinal tract but also in other organs, with Klebsiella pneumoniae serotype K1 resulting in a liver abscess (KLA) through oral inoculation in mice. However, the relationship between gastric microbiota and the extra-gastrointestinal KLA infection is not clear. In our study, a 454 pyrosequencing analysis of the bacterial 16S rRNA gene shows that the composition of gastric mucosal microbiota in mice with or without KLA infection varies greatly after oral inoculation with K. pneumoniae serotype K1 isolate. Interestingly, only several bacteria taxa show a significant change in gastric mucosal microbiota of KLA mice, including the decreased abundance of Bacteroides, Alisptipes and increased abundance of Streptococcus. It is worth noting that the abundance of Klebsiella exhibits an obvious increase in KLA mice, which might be closely related to KLA infection. At the same time, the endogenous antibiotics, defensins, involved in the regulation of the bacterial microbiota also show an increase in stomach and intestine. All these findings indicate that liver abscess caused by K. pneumoniae oral inoculation has a close relationship with gastric microbiota, which might provide important information for future clinical treatment.
The State of Research on Antimicrobial Activity of Cold Plasma
Authors: NIEDŹWIEDŹ IWONA , WAŚKO ADAM , PAWŁAT JOANNA , POLAK-BERECKA MAGDALENA 
Published: June 2019

Abstract
Microbiological contamination is a big challenge to the food industry, medicine, agriculture, and environmental protection. For this reason, scientists are constantly looking for alternative methods of decontamination, which ensure the effective elimination of unwanted biological agents. Cold plasma is a new technology, which due to its unique physical and chemical properties becomes a point of interest to a growing group of researchers. The previously conducted experiments confirm its effective action, e.g. in the disinfection of skin wounds, air, and sewage treatment, as well as in food preservation and decontamination. The reactive compounds present in the plasma: high-energy electrons, ionized atoms and molecules, and UV photons are the key factors that cause an effective reduction in the number of microorganisms. The mechanism and effectiveness of the cold plasma are complex and depend on the process parameters, environmental factors and the type and properties of the microorganisms that are to be killed. This review describes the current state of knowledge regarding the effectiveness of the cold plasma and characterizes its interaction with various groups of microorganisms based on the available literature data.
Mutagenic effects of ultraviolet (UV-C) irradiation on the anatomy of three species of Capsicum
Authors: Kareem KA Abdul , Olobatoke TJ , Rahaman AA Abdul , Mustapha OT 
Published: June 2019

UV radiant seedlings of Capsicum annuum, C. Chinenese and Capsicum frutescens were studied anatomically to observe the UV effects on the leaf epidermis, stem and root ultrastructures. While there is a higher percentage of stomatal index in the UV-exposed plants compared to the controlled, unexposed plants, there is no correlation in the stomatal density and stomatal size between the exposed and unexposed plants to the ultraviolet irradiation. There was also no correlation between the stomatal size and the stomatal density in both treatments (exposed and unexposed) in all the plants. Significant differences were observed in the stomatal index on both leaf surfaces between the exposed and controlled plants of C. frutescens and C. annuum. Cell walls of the stem and root wereobserved to be thicker in the UV-exposed plants.
Bangladesh J. Sci. Ind. Res.54(2), 111-116, 2019
In-vitro and in-vivo antibacterial effect of Croton lobatus Linnaeus L. on two days post surgical wounds in rats
Authors: Kilani MA , Hassan AZ , Fadason ST , Obalowu AM , Aliyu A , Kilani HM Badmus 
Published: June 2019

Phytochemical constituents of Croton lobatus L. (C. lobatus) water extracts and quantitative analysis were carried out following standard procedures. The antibacterial activity against Staphylococcus aureus (ATCC 33591); Streptococcus Spp; Pseudomonas aeruginosa (ATCC 9028); Proteus vulgaris; Escherichia coli (ATCC 43895); and Salmonella Spp (ATCC 4932) was carried out at the concentration of 0.5g/mL, 0.05 g/mL and 0.00 5g/mL of water. In vivo antimicrobial assay was carried out by creating four wounds of 0.5 by 0.5 cm on dorsal surface of a male albino rat under anesthesia. The wounds were left for 48 hrs, after which they were accessed and samples were collected for culture, identification and colony forming unit counts (CFU). Respective treatment using dried C. lobatus, C. lobatus (water extract), Physiological saline solution and Cicatrin powder was carried out and samples were collected at day one, three, five and seven after initiation of treatments for CFU counts on nutrient and MacConkey agar. The phytochemical studies revealed that C. lobatus contains carbohydrates, glycoside, saponins, steroids, triterpenes, flavonoids, alkaloids and tannins. Croton lobatus L. showed a dose dependent activity against micro organisms with C. lobatus 0.5 inhibited the growth of most bacteria at the zone of inhibition ≤ 21mm. This was also supported by in vivo antimicrobial assay. Secondary metabolite tannins, triterpenoids, flavonoids, crotonic acids and saponin were responsible for its antimicrobial activity against the tested microorganisms thereby supporting its usage by the traditional medicine practitioner in Nigeria to treat chronic wounds.
Bangladesh J. Sci. Ind. Res.54(2), 139-146, 2019
Health Problems of Elderly Patients attending Combined Military Hospital, Dhaka
Authors: Kabir SM Humayun , Islam Md Ziaul , Begum Masuda , Ahmed Masud , Mohsin Mohammad , Jahan Ishrat , Haque SM Shahidul 
Published: June 2019

Introduction: Health problems of elderly are an emerging health burden throughout the world. Bangladesh is currently undergoing a demographic transition and the proportion of the population of 60 years and older is increasing rapidly. Health care providers and policymakers are highly concerned with this burning issue.
Objective: To know the disease pattern among the elderly patients in Combined Military Hospital, Dhaka.
Materials and Methods: This cross-sectional study was carried out from July 2015 to June 2016 among 152 elderly patients above 60 years of age admitted in Combined Military Hospital (CMH), Dhaka Cantonment. Data were collected by face-to-face interview with semi-structured questionnaire and checklist following purposive sampling technique. Analysis of data was done by Statistical Package for Social Science (SPSS, version 20.0).
Results: Mean age of the elderly was 72.06±4.56 years with the range of 60-80 years and majority (90.8%) of the elderly was male. Out of total 152 elderly patients, by occupation majority (31.6%) were in the business group followed by 30.3% in the retired group and 9.2 % in the housewife group. Average monthly family income was BDT 17927.63±7360.75 with the range of BDT 6000-35000. With initial complaints elderly patients reported to doctors in private chamber (38.2%), private hospital (25.6%) and Govt hospital (5.9%). Among all of the elderly patients, majority (21.1%) had Diabetes Mellitus followed by Rheumatoid Arthritis (17.6%), Asthma (12.5%), Cataract (11.2%), ENT problem (6.6%), Malignancy (5.9%) and Benign Enlargement of Prostate 8(5.3%).
Conclusion: The number of elderly people is expanding rapidly; it also presents multifaceted health problems and thus creates unique challenges for the national healthcareservices. Early identification of problem and ensuring the availability of health with economic and social support can have a control over the elderly health problems.
Journal of Armed Forces Medical College Bangladesh Vol.13(1) 2017: 8-12
A Comparative Study on Indomethacin Suppositories with Physical Therapy and Conventional Treatment Modalities for Lumbago Sciatica
Authors: Haque SM Shahidul , Miah Md Abdul Ali , Yousuf Mohammad , Rahman Md Fashiur , Rahman Wahida , Kabir SM Humayun , Haque Md Maksudul 
Published: June 2019

Introduction: Most of the patients with Lumbago sciatica can be managed conservatively in a primary care setting with the expectation of a good outcome. It is one of the most common causes of pain and disability pertinent to lumbar spine. There are many alternative forms of treatment for this condition but the evidence of the efficacy of Indomethacin suppositories is yet to be established from clinical trials. Though several interventions are used in combination with physical therapy but indomethacin suppositories is commonly employed as a first line agent in the management of lumbago sciatica.
Objective: To ascertain whether a comparative effect of Indomethacin suppositories with other conventional treatment modalities produce a significant difference in the outcome.
Materials and Methods: This experimental clinical study was carried out at the Department of Physical Medicine, Combined Military Hospital (CMH), Dhaka and Department of Physical Medicine and Rehabilitation BSMMU, from 01 September 2015 to 28 February 2016. A total of 153 patients were randomly selected for trial and they were divided by lottery into 3 Groups. Group-“A” received Indomethacin suppositories with physical exercise and patients were 54 in number. 'Group-B' received NSAIDs (except indomethacin suppositories), SWD /MWD/IFT/TENS and patients were 47. In 'Group-C' there were 52 patients who received counseling with only physical exercises. Each Group received above-mentioned treatment modalities 5 days in a week for 6 weeks.
Results: The results were observed and noted with the help of visual analogue scale (VAS). The highest significant improvement was observed in 'Group-A' throughout the whole treatment period. The significant difference of improvement was found between groups finally in week 4 (W4) to week 6 (W6). The group received Indomethacin suppositories with physical exercise shows improvement of patients with lumbago sciatica.
Conclusion: The use of Indomethacin suppositories with physical therapy and the conventional modalities of treatment comparison help the physician to provide treatment facilities. The result was found better in groups those who received Indomethacin suppositories (Group-A) rather than those Groups who received other conventional treatment modalities.
Journal of Armed Forces Medical College Bangladesh Vol.13(1) 2017: 28-32
Operative versus Conservative Treatment for the Management of Displaced Midshaft Clavicle Fractures in Adults
Authors: Haque Md Maksudul , Ahmed Masud , Hossain Md Zakir , Faisal Md Ali , Islam Mohammad Saiful , Haque SM Shahidul , Tarik Md Monowar , Russel Mohammad 
Published: June 2019

Introduction: Displaced fractures of mid third clavicle are common in young athletic population and choice of management is still controversial. Recent studies have shown that these fractures do not have favourable outcomes with non-operative management and nonunion rates could be as high as 20%, in addition, there is symptomatic malunion with shortening.
Objective: To compare the results of the operative versus that of conservative treatment for the management of displaced midshaft clavicular fractures.
Materials and Methods: This prospective cross-sectional study was carried out in Orthopaedic and Trauma center, CMH, Dhaka, from the period of January 2014 to December 2016 to compare results of open reduction and internal fixation by plating with that of conservative management. Total 60 patients (30 in each group) were analyzed in terms of fracture union and functional outcome. Patients in the non-operative group were managed by triangular sling with or without strapping whereas in the operative group fractures were reduced and fixed with a contoured reconstruction plate. Patients were actively followed up for 12 months and functional outcome was measured by Rowe and Oxford shoulder scoring system. Complications were monitored clinically and radiologically.
Results: All fractures in the operated group united compared with thirteen cases of symptomatic malunion (43.33%) in the non-operated group which is statistically significant. Rowe and Oxford scores was significantly higher in the operated group than the non-operated group in every occasion of follow-up. There was no major complication of surgery. In one patient (3.33%) plate had to be removed for hardware irritation and prominence.
Conclusion: Open reduction and internal plate fixation in acute displaced midshaft clavicular fractures resulted in improved outcome decreased rate of non-union and symptomatic mal-union compared with non-operative treatment
Journal of Armed Forces Medical College Bangladesh Vol.13(1) 2017: 81-85
Role of Resistance Index in Differentiation of Benign and Malignant Breast Masses Compared with Histopathological Diagnosis
Authors: Amin Mashah Binte , Yasmin Tarana , Sarkar Samar C , Rekha Khaleda Parvin , Leeba Rushaida Haque , Akhter Nasima 
Published: June 2019

Background: Breast tumor remains a worldwide public health problem for women. Linear array sonography is currently one of the main diagnostic methods for detecting breast lumps. Duplex color Doppler sonography has been helpful to distinguish malignant from benign breast disease and also to predict the disease prognosis.
Objectives: The purpose of this study was to evaluate the role of spectral Doppler as a method to measure the vascular resistance index in differential diagnosis between the benign and malignant breast neoplasm.
Materials and Methods: This cross-sectional study was carried out in department of Radiology & Imaging of Enam Medical College & Hospital during January 2015 to December 2017. Sonography was done in 153 women having breast lumps. Among them histopathology was done in 105 cases. Ultrasonographic findings and histopathological report analyses were done using SPSS 13.0.
Results: The study was done in 105 women with mean age 31.6 ± 4.5 years. On ultrasonogram, lesions were diagnosed as benign in 65 (62%) cases and malignant in 40 (38%) cases. Out of sonographically diagnosed 65 benign lesions having peripheral vascularity and decreased RI, 63 (97%) were proved benign histopathologically. With these criteria for diagnosis of benign lesion, sensitivity was 94%, specificity 92%, positive predictive value 95%, negative predictive value 89% and accuracy 93%. Sonographically 40 lesions were diagnosed as malignant. Among them 37 (92.5%) cases were proven malignant histopathologically and showed central vascularity and increased RI having sensitivity of 92%, specificity 94%, positive predictive value 89%, negative predictive value 95% and accuracy 93%.
Conclusion: The analysis of vascular resistance index combined with findings on grayscale sonographic images correlates well with histopathological reports and can be of great assistance in the assessment of breast masses with high sensitivity and specificity.
J Enam Med Col 2019; 9(2): 97-103
Congenital Cytomegalovirus Infection: Evaluation and Management
Authors: Benzamin Md , Rahman Md Mizanur , Rukunuzzaman Md , Karim ASM Bazlul 
Published: June 2019

Congenital cytomegalovirus (CMV) infection is the most common congenital infection worldwide and most individuals are eventually exposed to this agent. In developing countries the seroprevalence in women of reproductive age approximates 100%. Cytomegalovirus (CMV) infection has great importance to obstetriciangynecologists and pediatricians. Despite the heavy disease burden, CMV infection is severely under-diagnosed because the majority (approximately 80%) of affected mothers are asymptomatic. The clinical manifestations of congenital CMV infection vary widely, from asymptomatic infection to potentially life-threatening disseminated disease. Prenatal diagnosis of fetal CMV infection can be made by testing amniotic fluid for cytomegalovirus by amniocentesis. Diagnosis of congenital cytomegalovirus infection in neonates should include real-time PCR of saliva, urine, or both, as soon as possible after birth. Antiviral therapy is not routinely recommended for congenital cytomegalovirus infection. Neonates with life-threatening infection and moderately to severely symptomatic congenital cytomegalovirus disease, CNS involvement is considered for immediate treatment that should be initiated within first month of life.
J Enam Med Col 2019; 9(2): 116-126
USAHA PEMBERDAYAAN EKONOMI MASYARAKAT MISKIN YANG MEMILIKI TANGGUNGAN ANAK PENYANDANG CACAT MELALUI KEGIATAN IbM
Authors: Arvianti Eri Yusnita , Lestari Sri Umi , Laiyan Macaria Theresia 
Published: June 2019

Fenomena kemiskinan di kalangan masyarakat yang memiliki tanggungan anak cacat merupakan masalah yang perlu segera diatasi. Banyak sekali anak penyandang cacat yang tidak bisa mengenyam bangku pendidikan karena kondisi ekonomi orang tua mereka yang tidak mampu. Kondisi seperti ini timbul karena masyarakat miskin tidak memiliki akses, kemampuan finansial dan ketrampilan yang cukup dalam mengatasi masalah tersebut.Keluarga penyandang cacat Ridho Eko Atmojo maupun Leni Nurhayati merupakan dua contoh keluarga yang memerlukan bimbingan dan bantuan modal. Rido Eko memiliki kelainan bisu dan tuli, sedangkan Leni memiliki kelainan epilepsy dan retardasi mental ringan. Melalui kegiatan Ipteks bagi masyarakat (IbM) berupa usaha jualan sayur keliling dan beternak ayam kampung ini diharapkan terjadi peningkatan produktivitas dan kemandirian keluarga mitra baik secara ekonomi mapun sosial, juga pemberian rehabilitasi medis si penyandang cacat agar mampu berperan dalam lingkungan sosialnya .Hasilnya setelah mendapatkan bantuan dari program IbM Keluarga Agus Susanto (Ridho Eko Atmojo) dan Keluarga Setiono (Leni Nurhayati) semakin meningkat produktifitasnya sehingga mampu memperbaiki taraf kehidupan ekonomi dan sosialnya menuju kemandirian ekonomi keluarga. Hasil analisis kelayakan usaha untuk jualan sayur keliling adalah sebesar 1.47 dan usaha beternak ayam sebesar 1.16 sehingga dua kegiatan usaha tersebut layak untuk diusahakan. Pemberian rehab medis memberikan efek positip terhadap tingkat kesehatan kedua anak mitra.Keyword : Miskin, Anak Cacat, Pemberdayaan.
CONTINGENCY LEADERSHIP THEORY / PENDEKATAN SITUASIONAL
Authors: Zulaihah Ifatun 
Published: June 2019

Paper ini mencoba menelaah konsep kepemimpinan situasional yang dipelopori diantaranya oleh Hersey and Blanchard, kemudian berkembang lagi teori Fiedler serta teori Path Goal. Dari teori tersebut masing-masing menganalisis bahwa kepemimpinan tidak hanya terbentuk oleh kepribadian dari pemimpin itu saja, namun kepemimpinan terbentuk dari lingkungan serta faktor-faktor yang mempengaruhi keberhasilan dari situasi sebuah organisasi agar mencapai tujuan yang dimaksud. Secara singkatnya teori tersebut menggunakan analisis kuantitatif dalam proses mempengaruhi bawahan-bawahannya dengan menggunakan variable-variable yang sudah terukur sedemikian rupa.