bluuebunny/crossref_metadata_2025_split huggingface dataset:
Field demonstration of a semiochemical treatment that enhances Diorhabda carinulata biological control of Tamarix spp.
Authors: Gaffke Alexander M. , Sing Sharlene E. , Dudley Tom L. , Bean Daniel W. , Russak Justin A. , Mafra-Neto Agenor , Peterson Robert K. D. , Weaver David K. 
Published: September 2019

AbstractThe northern tamarisk beetle Diorhabda carinulata (Desbrochers) was approved for release in the United States for classical biological control of a complex of invasive saltcedar species and their hybrids (Tamarix spp.). An aggregation pheromone used by D. carinulata to locate conspecifics is fundamental to colonization and reproductive success. A specialized matrix formulated for controlled release of this aggregation pheromone was developed as a lure to manipulate adult densities in the field. One application of the lure at onset of adult emergence for each generation provided long term attraction and retention of D. carinulata adults on treated Tamarix spp. plants. Treated plants exhibited greater levels of defoliation, dieback and canopy reduction. Application of a single, well-timed aggregation pheromone treatment per generation increased the efficacy of this classical weed biological control agent.
Impact of cleaning and disinfection procedures on microbial ecology and Salmonella antimicrobial resistance in a pig slaughterhouse
Authors: Bridier Arnaud , Le Grandois Patricia , Moreau Marie-Hélène , Prénom Charleyne , Le Roux Alain , Feurer Carole , Soumet Christophe 
Published: September 2019

AbstractTo guarantee food safety, a better deciphering of ecology and adaptation strategies of bacterial pathogens such as Salmonella in food environments is crucial. The role of food processing conditions such as cleaning and disinfection procedures on antimicrobial resistance emergence should especially be investigated. In this work, the prevalence and antimicrobial resistance of Salmonella and the microbial ecology of associated surfaces communities were investigated in a pig slaughterhouse before and after cleaning and disinfection procedures. Salmonella were detected in 67% of samples and isolates characterization revealed the presence of 15 PFGE-patterns belonging to five serotypes: S.4,5,12:i:-, Rissen, Typhimurium, Infantis and Derby. Resistance to ampicillin, sulfamethoxazole, tetracycline and/or chloramphenicol was detected depending on serotypes. 16S rRNA-based bacterial diversity analyses showed that Salmonella surface associated communities were highly dominated by the Moraxellaceae family with a clear site-specific composition suggesting a persistent colonization of the pig slaughterhouse. Cleaning and disinfection procedures did not lead to a modification of Salmonella susceptibility to antimicrobials in this short-term study but they tended to significantly reduce bacterial diversity and favored some genera such as Rothia and Psychrobacter. Such data participate to the construction of a comprehensive view of Salmonella ecology and antimicrobial resistance emergence in food environments in relation with cleaning and disinfection procedures.
Patched1 haploinsufficiency severely impacts intermediary metabolism in the skin of Ptch1+/−/ODC transgenic mice
Authors: Li Changzhao , Mishra Bharat , Kashyap Mahendra , Weng Zhiping , Andrabi Shaida A. , Mukhtar Shahid M. , Kim Arianna L. , Bickers David R. , Kopelovich Levy , Athar Mohammad 
Published: September 2019

AbstractThe study of dominantly heritable cancers has provided insights about tumor development. Gorlin syndrome (GS) is an autosomal dominant disorder wherein affected individuals develop multiple basal cell carcinomas (BCCs) of the skin. We developed a murine model of Ptch1 haploinsufficiency on an ornithine decarboxylase (ODC) transgenic background (Ptch1+/−/ODCt/C57BL/6) that is more sensitive to BCCs growth as compared with Ptch1+/+/ODCt/C57BL/6 littermates. Ptch1+/−/ODCt/C57BL/6 mice show an altered metabolic landscape in the phenotypically normal skin, including restricted glucose availability, restricted ribose/deoxyribose flow and NADPH production, an accumulation of α-ketoglutarate, aconitate, and citrate that is associated with reversal of the tricarboxylic acid cycle, coupled with increased ketogenic/lipogenic activity via acetyl-CoA, 3-hydroybutyrate, and cholesterol metabolites. Also apparent was an increased content/acetylation of amino-acids, glutamine and glutamate, in particular. Accordingly, metabolic alterations due to a single copy loss of Ptch1 in Ptch1+/−/ODCt/C57BL/6 heterozygous mice may provide insights about the cancer prone phenotype of BCCs in GS patients, including biomarkers/targets for early intervention.
Germline variability and tumor expression level of ribosomal protein gene RPL28 are associated with survival of metastatic colorectal cancer patients
Authors: Labriet Adrien , Lévesque Éric , Cecchin Erika , De Mattia Elena , Villeneuve Lyne , Rouleau Michèle , Jonker Derek , Couture Félix , Simonyan David , Allain Eric P. , Buonadonna Angela , D’Andrea Mario , Toffoli Giuseppe , Guillemette Chantal 
Published: September 2019

AbstractThis study investigated the potential of single nucleotide polymorphisms as predictors of survival in two cohorts comprising 417 metastatic colorectal cancer (mCRC) patients treated with the FOLFIRI (folinic acid, 5-fluorouracil and irinotecan) regimen. The rs4806668G > T of the ribosomal protein gene RPL28 was associated with shorter progression-free survival and overall survival by 5 and 9 months (P = 0.002), with hazard ratios of 3.36 (P < 0.001) and 3.07 (P = 0.002), respectively. The rs4806668T allele was associated with an increased RPL28 expression in transverse normal colon tissues (n = 246, P = 0.007). RPL28 expression was higher in colorectal tumors compared to paired normal tissues by up to 124% (P < 0.001) in three independent datasets. Metastatic cases with highest RPL28 tumor expression had a reduced survival in two datasets (n = 88, P = 0.009 and n = 56, P = 0.009). High RPL28 was further associated with changes in immunoglobulin and extracellular matrix pathways. Repression of RPL28 reduced proliferation by 1.4-fold to 5.6-fold (P < 0.05) in colon cancer HCT116 and HT-29 cells. Our findings suggest that the ribosomal RPL28 protein may influence mCRC outcome.
Isolation, characterization and identification of antibiofouling metabolite from mangrove derived Streptomyces sampsonii PM33
Authors: Gopikrishnan Venugopal , Radhakrishnan Manikkam , Shanmugasundaram Thangavel , Ramakodi Meganathan P. , Balagurunathan Ramasamy 
Published: September 2019

AbstractIn this study, we report the production, bioassay guided isolation and identification of antibiofouling metabolite from mangrove derived actinobacterium, Streptomyces sampsonii (PM33). The actinobacterial strain PM33 yields maximum amount of antifouling compounds through agar surface fermentation. In optimization, carbohydrates such as glucose, fructose and xylose, are suitable for maximum production of the active compound. In addition, other compounds such as malt extract, glutamine, and sodium chloride concentrations (2.5, 5 and 7.5%) and parameters such as pH 7.0 and temperature range 30 °C to 40 °C enhanced the production of antifouling metabolite. The antifouling metabolite was extracted in ethyl acetate. TLC and bioautography was used to separate and detect the antifouling metabolite present in the crude extract. The physico chemical features revealed that the antifouling metabolite PM33 – B as taxifolin (C15H12O7). The purified taxifolin was found to be active against biofouling bacteria, algal spore germination and mollusc foot adherence, respectively. Toxicity nature of taxifolin was also determined by adopting zebrafish embryos. The taxifolin isolated from mangrove-derived Streptomyces sampsonii PM33 is a promising candidate for the development of eco-friendly antifouling preparation.
Pre-treatment with the CDK4/6 inhibitor palbociclib improves the efficacy of paclitaxel in TNBC cells
Authors: Cretella Daniele , Fumarola Claudia , Bonelli Mara , Alfieri Roberta , La Monica Silvia , Digiacomo Graziana , Cavazzoni Andrea , Galetti Maricla , Generali Daniele , Petronini Pier Giorgio 
Published: September 2019

AbstractTriple Negative Breast Cancer (TNBC) is a challenging disease due to the lack of druggable targets; therefore, chemotherapy remains the standard of care and the identification of new targets is a high clinical priority. Alterations in the components of the cell cycle machinery have been frequently reported in cancer; given the success obtained with the CDK4/6 inhibitor palbocicib in ER-positive BC, we explored the potential of combining this drug with chemotherapy in Rb-positive TNBC cell models. The simultaneous combination of palbociclib with paclitaxel exerted an antagonistic effect; by contrast, the sequential treatment inhibited cell proliferation and increased cell death more efficaciously than single treatments. By down-regulating the E2F target c-myc, palbociclib reduced HIF-1α and GLUT-1 expression, and hence glucose uptake and consumption both under normoxic and hypoxic conditions. Importantly, these inhibitory effects on glucose metabolism were enhanced by palbociclib/paclitaxel sequential combination; the superior efficacy of such combination was ascribed to the ability of paclitaxel to inhibit palbociclib-mediated induction of AKT and to further down-regulate the Rb/E2F/c-myc signaling. Our results suggest that the efficacy of standard chemotherapy can be significantly improved by a pre-treatment with palbociclib, thus offering a better therapeutic option for Rb-proficient TNBC.
Rich observations of local and regional infrasound phases made by the AlpArray seismic network after refinery explosion
Authors: Fuchs Florian , Schneider Felix M. , Kolínský Petr , Serafin Stefano , Bokelmann Götz 
Published: September 2019

AbstractOn September 1st, 2018 a devastating explosion occurred on the facility of an oil refinery near Ingolstadt, Germany. We analyzed data of 400 permanent and temporary seismic stations and find strong seismo-acoustic signals on more than 80 seismic stations. The infrasound signal is detectable on seismic stations within 10–350 km from the source, with 40 km spatial resolution. We confirm the explosion site both by the seismic and seismo-acoustic arrivals. Apart from seismic P- and S-waves, we identified three separate acoustic phases with celerities of 332, 292, and 250 m/s, respectively, each of which has a particular spatial pattern of positive detections at the ground. Seismo-acoustic amplitudes are strongly affected by the type of seismic installation but still allow insight into regional infrasound attenuation. Our observations likely represent tropospheric, stratospheric, and thermospheric phases. We performed 3D acoustic ray tracing to validate our findings. Tropospheric and thermospheric arrivals are to some extent reproduced by the atmospheric model. However, ray tracing does not predict the observed acoustic stratospheric ducts. Our findings suggest that small-scale variations had considerable impact on the propagation of infrasound generated by the explosion.
Biodegradation influence on alkylphenanthrenes in oils from Bongor Basin, SW Chad
Authors: Huang Haiping , Li Zhiqiang , Wen Zhigang , Han Denglin , Pan Renfang 
Published: September 2019

AbstractOil samples from the Bongor Basin, SW Chad have been geochemically characterized to investigate the biodegradation influence on alkylphenanthrenes. Concentrations of C0–3-alkylphenanthrenes (C0–3Ps) increase markedly after level 6 biodegradation due to the removal of other vulnerable components, decrease sharply after level 7 biodegradation and approach to absence at level 8. Phenanthrene appears to have higher ability to resist biodegradation than C1–3Ps at certain biodegradation levels (≤level 7) due to demethylation, which has been inferred as a possible reaction process during biodegradation of the aromatic hydrocarbons. The enrichment of non-alkylated phenanthrene in biodegraded oils makes biodegradation assessment complicated on the basis of alkylphenanthrene distributions. Individual isomers in alkylphenanthrenes exhibit variable ability to resist biodegradation influence. While certain isomers do show higher ability to resist biodegradation than others, no uniform biodegradation sequence can be established. Meanwhile, the biodegradation susceptibility between hopanes and alkylphenanthrenes varies greatly in different samples. The biodegradation systematics of alkylphenanthrenes proves to be highly complex, which may be indicative of the multiple charges and mixing during biodegradation.
Effect of explant source, perlite nanoparticles and TiO2/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum
Authors: Ebadollahi R. , Jafarirad S. , Kosari-Nasab M. , Mahjouri S. 
Published: September 2019

AbstractIt appears that the biologically-synthesized nanoparticles (NPs) have potential to perform as effective elicitors for the production of valuable secondary metabolites in plants. Besides, it has been reported that the toxicity of the biologically-synthesized NP is not as much as that of the chemically-synthesized NPs. Therefore, it is necessary to test their advantages aspects. In this study, the physical synthesis of perlite NPs and biologically-synthesis of TiO2/perlite nanocomposites (NCs) were conducted. Subsequently, their effects and explant source influence on the growth characteristics and secondary metabolite profiles of Hypericum perforatum callus cultures were evaluated. According to the obtained results, morphology of the synthesized perlite NPs and TiO2/perlite NCs were mesoporous and spherical with sizes ranging about 14.51–23.34 and 15.50–24.61 nm, respectively. Addition of perlite NPs and TiO2/perlite NCs to the culture medium at the concentration range of 25–200 mg/L showed no adverse impacts on the growth characteristics of H. perforatum calli. According to the GC-MS analysis, the stress caused by perlite NPs and TiO2/perlite NCs led to an increase in the variety, amount and number of volatile compounds. The calli obtained from in vitro grown plants produced more volatile compounds relative to the calli obtained from field grown plants under the nanomaterial stress conditions. The production of hypericin and pseudohypericin were also determined in the callus cultures under desired nanomaterials elicitation. Accordingly, our results suggest that perlite NPs and TiO2/perlite NCs can possibly be considered as effective elicitors for the production of volatile compounds, hypericin, and pseudohypericin in callus cultures of H. perforatum.
Synthesis and characterization of CaSr-Metal Organic Frameworks for biodegradable orthopedic applications
Authors: Joseph Naomi , Lawson Harrison D. , Overholt Kalon J. , Damodaran Krishnan , Gottardi Riccardo , Acharya Abhinav P. , Little Steven R. 
Published: September 2019

AbstractMetal-organic frameworks (MOFs) formed from metals and organic ligands, are crystalline materials that are degradable in aqueous medium, and capable of releasing Ca and Sr ions. In this manuscript, the ability of MOFs to degrade and release osteogenic Ca and Sr ions was investigated. MOFs were generated by choosing osteoinductive Ca and Sr metals, and an organic ligand 1,3,5 tricarboxylicbenzene (H3BTC) as a linker. These MOFs were able to induce in vitro biomineralization from pre-osteoblastic MC3T3 cells and human mesenchymal stem cells (hMSCs). Moreover, these MOFs (when loaded with dimethyloxalylglycine (DMOG)) induced vascular endothelial production from hMSCs. qRT-PCR analysis performed on hMSCs (isolated from femoral heads of patients undergoing joint arthroplasty) treated with MOFs crystals suggested that the CaSr-MOFs by themselves can upregulate osteogenic genes in hMSCs, which is the first time to our knowledge that this has been observed from MOFs.
Efficient partition of integer optimization problems with one-hot encoding
Authors: Okada Shuntaro , Ohzeki Masayuki , Taguchi Shinichiro 
Published: September 2019

AbstractQuantum annealing is a heuristic algorithm for solving combinatorial optimization problems, and hardware for implementing this algorithm has been developed by D-Wave Systems Inc. The current version of the D-Wave quantum annealer can solve unconstrained binary optimization problems with a limited number of binary variables. However, the cost functions of several practical problems are defined by a large number of integer variables. To solve these problems using the quantum annealer, integer variables are generally binarized with one-hot encoding, and the binarized problem is partitioned into small subproblems. However, the entire search space of the binarized problem is considerably larger than that of the original integer problem and is dominated by infeasible solutions. Therefore, to efficiently solve large optimization problems with one-hot encoding, partitioning methods that extract subproblems with as many feasible solutions as possible are required. In this study, we propose two partitioning methods and demonstrate that they result in improved solutions.
Fine-tuning the photosynthetic light harvesting apparatus for improved photosynthetic efficiency and biomass yield
Authors: Friedland N. , Negi S. , Vinogradova-Shah T. , Wu G. , Ma L. , Flynn S. , Kumssa T. , Lee C.-H. , Sayre R. T. 
Published: September 2019

AbstractPhotosynthetic electron transport rates in higher plants and green algae are light-saturated at approximately one quarter of full sunlight intensity. This is due to the large optical cross section of plant light harvesting antenna complexes which capture photons at a rate nearly 10-fold faster than the rate-limiting step in electron transport. As a result, 75% of the light captured at full sunlight intensities is reradiated as heat or fluorescence. Previously, it has been demonstrated that reductions in the optical cross-section of the light-harvesting antenna can lead to substantial improvements in algal photosynthetic rates and biomass yield. By surveying a range of light harvesting antenna sizes achieved by reduction in chlorophyll b levels, we have determined that there is an optimal light-harvesting antenna size that results in the greatest whole plant photosynthetic performance. We also uncover a sharp transition point where further reductions or increases in antenna size reduce photosynthetic efficiency, tolerance to light stress, and impact thylakoid membrane architecture. Plants with optimized antenna sizes are shown to perform well not only in controlled greenhouse conditions, but also in the field achieving a 40% increase in biomass yield.
Prothrombin, alone or in complex concentrates or plasma, reduces bleeding in a mouse model of blood exchange-induced coagulopathy
Authors: Eltringham-Smith Louise J. , Yu Ruoying , Qadri Syed M. , Wang Yiming , Bhakta Varsha , Pryzdial Edward L. , Crosby Jeffrey R. , Ni Heyu , Sheffield William P. 
Published: September 2019

AbstractProthrombin complex concentrates (PCC) are fractionated plasma protein drugs that reverse warfarin anticoagulation. PCC may control more general bleeding. We sought to identify the dominant procoagulant factor in PCC in vivo. We tested PCC or coagulation factor (F) treatment in CD1 mice made coagulopathic by exchange of whole blood for washed red cells. Anesthetized mice were transfused with murine fresh-frozen plasma (mFFP), PCC, mixtures of human vitamin K-dependent proteins (VKDP) (prothrombin, FVII, FIX, or FX), or purified single human VKDP, immediately prior to tail transection (TT), liver laceration (LL), or intravascular laser injury (ILI). Plasma donor mice were treated with vehicle or control antisense oligonucleotide (ASO-CON) or ASO specific for prothrombin (FII) (ASO-FII) to yield mFFP or ASO-CON mFFP or ASO-FII mFFP. Blood losses were determined spectrophotometrically (TT) or gravimetrically (LL). Thrombus formation was quantified by intravital microscopy of laser-injured arterioles. PCC or four factor- (4F-) VKDP or prothrombin significantly reduced bleeding from TT or LL. Omission of prothrombin from 4F-VKDP significantly reduced its ability to limit bleeding. Mice transfused with ASO-FII mFFP demonstrated inferior haemostasis versus those transfused with ASO-FII following TT, LL, or ILI. Prothrombin is the dominant procoagulant component of PCC and could limit bleeding in trauma.
Exploring the link between microseism and sea ice in Antarctica by using machine learning
Authors: Cannata Andrea , Cannavò Flavio , Moschella Salvatore , Gresta Stefano , Spina Laura 
Published: September 2019

AbstractThe most continuous and ubiquitous seismic signal on Earth is the microseism, closely related to ocean wave energy coupling with the solid Earth. A peculiar feature of microseism recorded in Antarctica is the link with the sea ice, making the temporal pattern of microseism amplitudes different with respect to the microseism recorded in low-middle latitude regions. Indeed, during austral winters, in Antarctica the oceanic waves cannot efficiently excite seismic energy because of the sea ice in the Southern Ocean. Here, we quantitatively investigate the relationship between microseism, recorded along the Antarctic coasts, and sea ice concentration. In particular, we show a decrease in sea ice sensitivity of microseism, due to the increasing distance from the station recording the seismic signal. The influence seems to strongly reduce for distances above 1,000 km. Finally, we present an algorithm, based on machine learning techniques, allowing to spatially and temporally reconstruct the sea ice distribution around Antarctica based on the microseism amplitudes. This technique will allow reconstructing the sea ice concentration in both Arctic and Antarctica in periods when the satellite images, routinely used for sea ice monitoring, are not available, with wide applications in many fields, first of all climate studies.
Hyperglycemia induces spermatogenic disruption via major pathways of diabetes pathogenesis
Authors: Maresch Constanze C. , Stute Dina C. , Fleming Thomas , Lin Jihong , Hammes Hans-Peter , Linn Thomas 
Published: September 2019

AbstractDiabetes-induced hyperglycemia has previously been shown to impact on male sub-/infertility, however, still little is known about the underlying mechanisms. In the present study we have addressed three major biochemical pathways implicated in the pathogenesis of hyperglycemia induced organ damage (the advanced glycation end product (AGE) formation pathway, the diacylglycerol-protein kinase C pathway (PKC), and the polyol pathway) in both testis and epididymis of the Ins2Akita mouse model of Type 1 diabetes (T1DM). Hyperglycemia activated both the PKC and the polyol pathway in a significant and progressive manner within the testis, but not within the epididymis. While the AGE receptor was ubiquitiously expressed in the testis, concentrations of precursor methylglyoxal and AGE carboxymethyllysine were increased in both epididymis and testis in diabetic mice. However, AGEs did not activate intracellular pathways of ERK1, ERK2, Rela, Nrf-2, IkBkB, NFkB except CDC42, Akt1. In conclusion, two of the major pathways of hyperglycemia-induced organ damage were clearly activated within the testis of T1DM mice. This provides therapeutical opportunities in the treatment of diabetic male reproductive dysfunction.