bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neck-specific exercise may reduce radiating pain and signs of neurological deficits in chronic whiplash - Analyses of a randomized clinical trial
Authors: Landén Ludvigsson Maria , Peterson Gunnel , Peolsson Anneli 
Published: August 2018

AbstractUp to 90% of people with neurological deficits following a whiplash injury do not recover and cervical muscle dysfunction is common. The aim of this multicentre, randomized controlled trial was to examine whether two versions of neck-specific exercise or prescription of physical activity (PPA) can improve radiating arm pain and clinical signs that can be associated with neurological deficits in people with chronic whiplash associated disorders (WAD). Participants with chronic WAD, arm symptoms and signs associated with neurological deficits (n = 171) were randomized to: 12 weeks of neck-specific exercise without (NSE) or with a behavioural approach (NSEB), or PPA. Pain/bothersomeness frequency, six measures of arm pain/paraesthesia (VAS scales), and four clinical neurological tests were evaluated after 3 months. The NSE group reported the lowest frequency and lowest levels of arm pain, the highest proportion of participants with at least 50% pain reduction and the highest proportion of normal arm muscle force. The NSEB group reported increased normal tendon reflexes. No improvements were recorded for the PPA group. Neck-specific exercise may improve arm pain and decrease signs of neurological deficits, but the addition of a behavioural approach does not seem to be of additional benefit.
Klebsiella oxytoca expands in cancer cachexia and acts as a gut pathobiont contributing to intestinal dysfunction
Authors: Pötgens Sarah A. , Brossel Hélène , Sboarina Martina , Catry Emilie , Cani Patrice D. , Neyrinck Audrey M. , Delzenne Nathalie M. , Bindels Laure B. 
Published: August 2018

AbstractCancer cachexia is a complex multi-organ syndrome characterized by body weight loss, weakness, muscle atrophy and fat depletion. With a prevalence of 1 million people in Europe and only limited therapeutic options, there is a high medical need for new approaches to treat cachexia. Our latest results highlighted microbial dysbiosis, characterized by a bloom inEnterobacteriaceaeand altered gut barrier function in preclinical models of cancer cachexia. They also demonstrated the potential of targeting the gut microbial dysbiosis in this pathology. However, the exact mechanisms underlying the gut microbiota-host crosstalk in cancer cachexia remain elusive. In this set of studies, we identifiedKlebsiella oxytocaas one of the mainEnterobacteriaceaespecies increased in cancer cachexia and we demonstrated that this bacteria acts as a gut pathobiont by altering gut barrier function in cachectic mice. Moreover, we propose a conceptual framework for the lower colonization resistance toK.oxytocain cancer cachexia that involves altered host gut epithelial metabolism and host-derived nitrate boosting the growth of the gut pathobiont. This set of studies constitutes a strong progression in the field of gut microbiota in cancer cachexia, by dissecting the mechanism of emergence of one bacterium,K.oxytoca, and establishing its role as a gut pathobiont in this severe disease.
Hybrid optical-electronic convolutional neural networks with optimized diffractive optics for image classification
Authors: Chang Julie , Sitzmann Vincent , Dun Xiong , Heidrich Wolfgang , Wetzstein Gordon 
Published: August 2018

AbstractConvolutional neural networks (CNNs) excel in a wide variety of computer vision applications, but their high performance also comes at a high computational cost. Despite efforts to increase efficiency both algorithmically and with specialized hardware, it remains difficult to deploy CNNs in embedded systems due to tight power budgets. Here we explore a complementary strategy that incorporates a layer of optical computing prior to electronic computing, improving performance on image classification tasks while adding minimal electronic computational cost or processing time. We propose a design for an optical convolutional layer based on an optimized diffractive optical element and test our design in two simulations: a learned optical correlator and an optoelectronic two-layer CNN. We demonstrate in simulation and with an optical prototype that the classification accuracies of our optical systems rival those of the analogous electronic implementations, while providing substantial savings on computational cost.
Quantifying emergence and self-organisation of Enterobacter cloacae microbial communities
Authors: Balaban Valeriu , Lim Sean , Gupta Gaurav , Boedicker James , Bogdan Paul 
Published: August 2018

AbstractFrom microbial communities to cancer cells, many such complex collectives embody emergent and self-organising behaviour. Such behaviour drives cells to develop composite features such as formation of aggregates or expression of specific genes as a result of cell-cell interactions within a cell population. Currently, we lack universal mathematical tools for analysing the collective behaviour of biological swarms. To address this, we propose a multifractal inspired framework to measure the degree of emergence and self-organisation from scarce spatial (geometric) data and apply it to investigate the evolution of the spatial arrangement of Enterobacter cloacae aggregates. In a plate of semi-solid media, Enterobacter cloacae form a spatially extended pattern of high cell density aggregates. These aggregates nucleate from the site of inoculation and radiate outward to fill the entire plate. Multifractal analysis was used to characterise these patterns and calculate dynamics changes in emergence and self-organisation within the bacterial population. In particular, experimental results suggest that the new aggregates align their location with respect to the old ones leading to a decrease in emergence and increase in self-organisation.
Humpback whale migrations to Antarctic summer foraging grounds through the southwest Pacific Ocean
Authors: Andrews-Goff V. , Bestley S. , Gales N. J. , Laverick S. M. , Paton D. , Polanowski A. M. , Schmitt N. T. , Double M. C. 
Published: August 2018

AbstractHumpback whale (Megaptera novaeangliae) populations typically undertake seasonal migrations, spending winters in low latitude breeding grounds and summers foraging in high latitude feeding grounds. Until recently, a broad scale understanding of whale movement has been derived from whaling records, Discovery marks, photo identification and genetic analyses. However, with advances in satellite tagging technology and concurrent development of analytical methodologies we can now detail finer scale humpback whale movement, infer behavioural context and examine how these animals interact with their physical environment. Here we describe the temporal and spatial characteristics of migration along the east Australian seaboard and into the Southern Ocean by 30 humpback whales satellite tagged over three consecutive austral summers. We characterise the putative Antarctic feeding grounds and identify supplemental foraging within temperate, migratory corridors. We demonstrate that Antarctic foraging habitat is associated with the marginal ice zone, with key predictors of inferred foraging behaviour including distance from the ice edge, ice melt rate and variability in ice concentration two months prior to arrival. We discuss the highly variable ice season within the putative foraging habitat and the implications that this and other environmental factors may have on the continued strong recovery of this humpback whale population.
Interaction of septin 7 and DOCK8 in equine lymphocytes reveals novel insights into signaling pathways associated with autoimmunity
Authors: Schauer Melanie , Kleinwort Kristina J. H. , Degroote Roxane L. , Wiedemann Carmen , Kremmer Elisabeth , Hauck Stefanie M. , Deeg Cornelia A. 
Published: August 2018

AbstractThe GTP-binding protein septin 7 is involved in various cellular processes, including cytoskeleton organization, migration and the regulation of cell shape. Septin 7 function in lymphocytes, however, is poorly characterized. Since the intracellular signaling role of septin 7 is dependent on its interaction network, interaction proteomics was applied to attain novel knowledge about septin 7 function in hematopoietic cells. Our previous finding of decreased septin 7 expression in blood-derived lymphocytes in ERU, a spontaneous animal model for autoimmune uveitis in man, extended the role of septin 7 to a potential key player in autoimmunity. Here, we revealed novel insights into septin 7 function by identification of DOCK8 as an interaction partner in primary blood-derived lymphocytes. Since DOCK8 is associated with important immune functions, our finding of significantly decreased DOCK8 expression and altered DOCK8 interaction network in ERU might explain changes in immune response and shows the contribution of DOCK8 in pathomechanisms of spontaneous autoimmune diseases. Moreover, our analyses revealed insights in DOCK8 function, by identifying the signal transducer ILK as a DOCK8 interactor in lymphocytes. Our finding of the enhanced enrichment of ILK in ERU cases indicates a deviant influence of DOCK8 on inter- and intracellular signaling in autoimmune disease.
Invisible Flashes Alter Perceived Sound Location
Authors: Delong Patrycja , Aller Máté , Giani Anette S. , Rohe Tim , Conrad Verena , Watanabe Masataka , Noppeney Uta 
Published: August 2018

AbstractInformation integration across the senses is fundamental for effective interactions with our environment. The extent to which signals from different senses can interact in the absence of awareness is controversial. Combining the spatial ventriloquist illusion and dynamic continuous flash suppression (dCFS), we investigated in a series of two experiments whether visual signals that observers do not consciously perceive can influence spatial perception of sounds. Importantly, dCFS obliterated visual awareness only on a fraction of trials allowing us to compare spatial ventriloquism for physically identical flashes that were judged as visible or invisible. Our results show a stronger ventriloquist effect for visible than invisible flashes. Critically, a robust ventriloquist effect emerged also for invisible flashes even when participants were at chance when locating the flash. Collectively, our findings demonstrate that signals that we are not aware of in one sensory modality can alter spatial perception of signals in another sensory modality.
Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA
Authors: Miyagi-Shiohira Chika , Nakashima Yoshiki , Kobayashi Naoya , Saitoh Issei , Watanabe Masami , Noguchi Hirofumi 
Published: August 2018

AbstractInduced pluripotent stem (iPS) cells have significant implications for overcoming most of the ethical issues associated with embryonic stem (ES) cells. Furthermore, our recent study demonstrated the generation of induced tissue-specific stem (iTS) cells by transient overexpression of the reprogramming factors using a plasmid combined with tissue-specific selection. In this study, we were able to generate RNA-based iTS cells that utilize a single, synthetic, self-replicating VEE-RF RNA replicon expressing four reprogramming factors (OCT4, KLF4, SOX2, and GLIS1). A single VEE-RF RNA transfection into mouse pancreatic tissue resulted in efficient generation of iTS cells from pancreas (iTS-P cells) with genetic markers of endoderm and pancreatic progenitors and differentiation into insulin-producing cells more efficiently than ES cells. Subcutaneous transplantation of iTS-P cells into immunodeficient mice resulted in no teratoma formation. Bisulfite genomic sequencing demonstrated that the promoters of Oct4 and Nanog remained partially methylated in iTS-P cells. We compared the global gene-expression profiles of ES cells, iTS-P cells, and pancreatic islets. Microarray analyses confirmed that the iTS-P cells were similar but not identical to ES cells compared with islets. These data suggest that iTS-P cells are cells that inherit numerous components of epigenetic memory from pancreas cells and acquire self-renewal potential. The generation of iTS cells may have important implications for the clinical application of stem cells.
Extracellular vesicles protect glucuronidase model enzymes during freeze-drying
Authors: Frank Julia , Richter Maximilian , de Rossi Chiara , Lehr Claus-Michael , Fuhrmann Kathrin , Fuhrmann Gregor 
Published: August 2018

AbstractExtracellular vesicles (EVs) are natural nanoparticles that play important roles in intercellular communication and are increasingly studied for biosignalling, pathogenesis and therapy. Nevertheless, little is known about optimal conditions for their transfer and storage, and the potential impact on preserving EV-loaded cargoes. We present the first comprehensive stability assessment of different widely available types of EVs during various storage conditions including −80 °C, 4 °C, room temperature, and freeze-drying (lyophilisation). Lyophilisation of EVs would allow easy handling at room temperature and thus significantly enhance their expanded investigation. A model enzyme, β-glucuronidase, was loaded into different types of EVs derived from mesenchymal stem cells, endothelial cells and cancer cells. Using asymmetric flow field-flow fractionation we proved that the model enzyme is indeed stably encapsulated into EVs. When assessing enzyme activity as indicator for EV stability, and in comparison to liposomes, we show that EVs are intrinsically stable during lyophilisation, an effect further enhanced by cryoprotectants. Our findings provide new insight for exploring lyophilisation as a novel storage modality and we create an important basis for standardised and advanced EV applications in biomedical research.
Polarization-sensitive tunable absorber in visible and near-infrared regimes
Authors: Lee Dasol , Han Sung Yong , Jeong Yeonggyo , Nguyen Duc Minh , Yoon Gwanho , Mun Jungho , Chae Jeonghoon , Lee Jae Hyuk , Ok Jong G. , Jung Gun Young , Park Hui Joon , Kim Kyunghoon , Rho Junsuk 
Published: August 2018

AbstractA broadband tunable absorber is designed and fabricated. The tunable absorber is comprised of a dielectric-metal-dielectric multilayer and plasmonic grating. A large size of tunable absorber device is fabricated by nano-imprinting method. The experimental results show that over 90% absorption can be achieved within visible and near-infrared regimes. Moreover, the high absorption can be controlled by changing the polarization of incident light. This polarization-sensitive tunable absorber can have practical applications such as high-efficiency polarization detectors and transmissive polarizer.
Density functional theory study of the role of benzylic hydrogen atoms in the antioxidant properties of lignans
Authors: Vo Quan V. , Nam Pham Cam , Bay Mai Van , Thong Nguyen Minh , Cuong Nguyen Duc , Mechler Adam 
Published: August 2018

AbstractAntioxidants are a diverse group of chemicals with proven health benefits and thus potential preventive medicine and therapeutic applications. While most of these compounds are natural products, determining their mechanism of radical scavenging and common motifs that contribute to antioxidant activity would allow the rational design of novel antioxidants. Here the origins of the antioxidant properties of ten natural products of the lignan family were studied in silico by calculating their thermochemical properties by using ROB3LYP/6-311++G(2df,2p)//B3LYP/6-311G(d,p) model chemistry. Three conditions were modelled: gas phase, ethanol and water solvents. The results allowed assigning the antioxidant activity to specific moieties and structural features of these compounds. It was found that the benzylic hydrogen atoms are the most likely to be abstracted to form radicals and hence define antioxidant properties in most of the studied compounds. The results also suggested that the most likely mechanism of HOO• radical scavenging differs by the key moiety: it is hydrogen atom transfer in case the benzylic C-H bonds, however it is proton coupled electron transfer in case of the compounds where O-H bonds are responsible for radical scavenging.
Impact of Comorbidities and Smoking on the Outcome in Aneurysmal Subarachnoid Hemorrhage
Authors: Hammer Alexander , Steiner Anahi , Ranaie Gholamreza , Yakubov Eduard , Erbguth Frank , Hammer Christian M. , Killer-Oberpfalzer Monika , Steiner Hans , Janssen Hendrik 
Published: August 2018

AbstractThe intention of this observational study is to show the significant impact of comorbidities and smoking on the outcome in aneurysmal subarachnoid hemorrhage (SAH). During this observational study 203 cases of treatment of ruptured intracranial aneurysms were analyzed. We examined and classified prospectively the 12 month outcome according to the modified Rankin Scale (mRS) considering retrospectively a history of smoking and investigated prospectively the occurrence of early and delayed cerebral ischemia between 2012 and 2017. Using logistic regression methods, we revealed smoking (odds ratio 0.21; p = 0.0031) and hypertension (odds ratio 0.18; p = 0.0019) to be predictors for a good clinical outcome (mRS 0–2). Age (odds ratio 1.05; p = 0.0092), WFNS Grade (odds ratio 6.28; p < 0.0001), early cerebral ischemia (ECI) (odds ratio 10.06; p < 0.00032) and delayed cerebral ischemia (DCI) (odds ratio 4.03; p = 0.017) were detected as predictors for a poor clinical outcome. Significant associations of occurrence of death with hypertension (odds ratio 0.12; p < 0.0001), smoking (odds ratio 0.31; p = 0.048), WFNS grade (odds ratio 3.23; p < 0.0001) and age (odds ratio 1.09; p < 0.0001), but not with ECI (p = 0.29) and DCI (p = 0.62) were found. Smoking and hypertension seem to be predictors for a good clinical outcome after aneurysmal SAH.
Nuclear connectin novex-3 promotes proliferation of hypoxic foetal cardiomyocytes
Authors: Hashimoto Ken , Kodama Aya , Sugino Miki , Yobimoto Tomoko , Honda Takeshi , Hanashima Akira , Ujihara Yoshihiro , Mohri Satoshi 
Published: August 2018

AbstractLoss of cardiomyocyte proliferative capacity after birth is a major obstacle for therapeutic heart regeneration in adult mammals. We and others have recently shown the importance of hypoxic in utero environments for active foetal cardiomyocyte proliferation. Here, we report the unexpected expression of novex-3, the short splice variant of the giant sarcomeric protein connectin (titin), in the cardiomyocyte nucleus specifically during the hypoxic foetal stage in mice. This nuclear localisation appeared to be regulated by the N-terminal region of novex-3, which contains the nuclear localisation signal. Importantly, the nuclear expression of novex-3 in hypoxic foetal cardiomyocytes was repressed at the postnatal stage following the onset of breathing and the resulting elevation of oxygen tension, whereas the sarcomeric expression remained unchanged. Novex-3 knockdown in foetal cardiomyocytes repressed cell cycle-promoting genes and proliferation, whereas novex-3 overexpression enhanced proliferation. Mechanical analysis by atomic force microscopy and microneedle-based tensile tests demonstrated that novex-3 expression in hypoxic foetal cardiomyocytes contributes to the elasticity/compliance of the nucleus at interphase and facilitates proliferation, by promoting phosphorylation-induced disassembly of multimer structures of nuclear lamins. We propose that novex-3 has a previously unrecognised role in promoting cardiomyocyte proliferation specifically at the hypoxic foetal stage.
Effect of probiotic Bifidobacterium bifidum G9-1 on the relationship between gut microbiota profile and stress sensitivity in maternally separated rats
Authors: Fukui Hirokazu , Oshima Tadayuki , Tanaka Yoshiki , Oikawa Yosuke , Makizaki Yutaka , Ohno Hiroshi , Tomita Toshihiko , Watari Jiro , Miwa Hiroto 
Published: August 2018

AbstractAlthough gut microbiota and early life events are likely involved in the development of irritable bowel syndrome (IBS), it remains unclear how these factors interact in the pathophysiology of IBS. In the present study, using rats subjected to maternal separation (MS) as a model of IBS, we investigated interrelationships among gut microbiota, stress susceptibility and intestinal permeability, and examined the effect of the probiotic Bifidobacterium bifidum G9-1 (BBG9-1) on those interrelationships. When compared with the controls at postnatal day 20, MS rats showed hypercorticosteronemia, enhanced intestinal permeability and changes in gut microbiota structure. All of these changes in MS rats were prevented by treatment with BBG9-1. Although the gut microbiota profile and basal serum corticosterone level did not differ between MS and control rats at postnatal day 56, MS rats showed hypersensitivity to restraint stress in terms of serum corticosterone level and fecal frequency. However, such hypersensitivity was not observed in MS rats treated with BBG9-1. These findings suggest that MS initiates the link between gut microbiota alteration and hypersensitivity to stress and that the triggering of this process can be prevented by the treatment with the probiotic BBG9-1.
UBXD1 is a mitochondrial recruitment factor for p97/VCP and promotes mitophagy
Authors: Bento Ana C. , Bippes Claudia C. , Kohler Corina , Hemion Charles , Frank Stephan , Neutzner Albert 
Published: August 2018

AbstractClearance of damaged mitochondria through mitophagy is critical for maintaining mitochondrial fidelity and the prevention of neurodegeneration. Here, we report on the UBX domain-containing, p97/VCP cofactor UBXD1/UBXN6/UBXDC2 and its role in mitophagy. Recognizing depolarized mitochondria via its C-terminal UBX domain, UBXD1 translocates to mitochondria in a Parkin-dependent manner. During Parkin-independent mitophagy, UBXD1 shows no mitochondrial translocation. Once translocated, UBXD1 recruits p97 to mitochondria via a bipartite binding motif consisting of its N-terminal VIM and PUB domains. Recruitment of p97 by UBXD1 only depends on the presence of UBXD1 on mitochondria without the need for further mitochondrial signals. Following translocation of UBXD1 to CCCP-depolarized mitochondria and p97 recruitment, formation of LC3-positive autolysosomes is strongly enhanced and autophagic degradation of mitochondria is significantly accelerated. Diminished levels of UBXD1 negatively impact mitophagic flux in Parkin-expressing cells after CCCP treatment. Thus, our data supports a model, whereby the p97 cofactor UBXD1 promotes Parkin-dependent mitophagy by specifically recognizing damaged mitochondria undergoing autophagic clearance.
Avian vision models and field experiments determine the survival value of peppered moth camouflage
Authors: Walton Olivia C. , Stevens Martin 
Published: August 2018

AbstractAnimal defensive coloration has long provided many important examples of evolution and adaptation. Of these, industrial melanism in the peppered moth is the classic textbook example of evolution in action, whereby dark and pale morphs suffer differential predation in polluted and unpolluted woodland based on their camouflage. Despite extensive work, a striking gap remains in that no study has ever objectively quantified their camouflage or related this directly to predation risk. Here we use image analysis and avian vision models to show that pale individuals more closely match lichen backgrounds than dark morphs. Artificial predation experiments in unpolluted woodland show 21% higher survival rates of pale than melanic individuals. Overall, we provide the strongest direct evidence to date that peppered moth morph frequencies stem from differential camouflage and avian predation, providing key support for this iconic example of natural selection.