bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving Permafrost Modeling by Assimilating Remotely Sensed Soil Moisture
Authors: Zwieback S. , Westermann S. , Langer M. , Boike J. , Marsh P. , Berg A. 
Published: February 2019

AbstractKnowledge of soil moisture conditions is important for modeling soil temperatures, as soil moisture influences the thermal dynamics in multiple ways. However, in permafrost regions, soil moisture is highly heterogeneous and difficult to model. Satellite soil moisture data may fill this gap, but the degree to which they can improve permafrost modeling is unknown. To explore their added value for modeling soil temperatures, we assimilate fine‐scale satellite surface soil moisture into the CryoGrid‐3 permafrost model, which accounts for the soil moisture's influence on the soil thermal properties and the surface energy balance. At our study site in the Canadian Arctic, the assimilation improves the estimates of deeper (>10 cm) soil temperatures during summer but not consistently those of the near‐surface temperatures. The improvements in the deeper temperatures are strongly contingent on soil type: They are largest for porous organic soils (30%), smaller for thin organic soil covers (20%), and they essentially vanish for mineral soils (only synthetic data available). That the improvements are greatest over organic soils reflects the strong coupling between soil moisture and deeper temperatures. The coupling arises largely from the diminishing soil thermal conductivity with increasing desiccation thanks to which the deeper soil is kept cool. It is this association of dry organic soils being cool at depth that lets the assimilation revise the simulated soil temperatures toward the actually measured ones. In the future, the increasing availability of satellite soil moisture data holds promise for the operational monitoring of soil temperatures, hydrology, and biogeochemistry.
Controls on Near‐Surface Hydraulic Conductivity in a Raised Bog
Authors: Morris Paul J. , Baird Andy J. , Eades Phil A. , Surridge Ben W. J. 
Published: February 2019

AbstractShallow water tables protect northern peatlands and their important carbon stocks from aerobic decomposition. Hydraulic conductivity, K, is a key control on water tables. The controls on K, particularly in degraded and restored peatlands, remain a subject of ongoing research. We took 29 shallow (~50 cm) peat cores from an estuarine raised bog in Wales, UK. Parts of the bog are in close‐to‐natural condition, while other areas have undergone shallow peat cutting for fuel and drainage, followed by restoration through ditch blocking. In the laboratory we measured horizontal (Kh) and vertical (Kv) hydraulic conductivity. We fitted linear multiple regression models to describe log10‐transformed Kh and Kv on the basis of simple, easy‐to‐measure predictors. Dry bulk density and degree of decomposition were the strongest predictors of Kh and Kv. Perhaps surprisingly, the independent effect of hummocks was to produce higher‐Kv peat than in lawns; while the independent effect of restored diggings was to produce higher‐K peat than in uncut locations. Our models offer high explanatory power for Kh (adjusted r2 = 0.740) and Kv (adjusted r2 = 0.787). Our findings indicate that generalizable predictive models of peat K, similar to pedotransfer functions for mineral soils, may be attainable. Kh and Kv possess subtly different controls that are consistent with the contrasting roles of these two properties in peatland water budgets. Our near‐surface samples show no evidence for the low‐K marginal peat previously observed in deeper layers at the same site, indicating that such structures may be less important than previously believed.
Immunoglobulin G modulation of the melanocortin 4 receptor signaling in obesity and eating disorders
Authors: Lucas Nicolas , Legrand Romain , Bôle-Feysot Christine , Breton Jonathan , Coëffier Moïse , Akkermann Kirsti , Järv Anu , Harro Jaanus , Déchelotte Pierre , Fetissov Sergueï O. 
Published: February 2019

AbstractMelanocortin 4 receptor (MC4R) plays a key role in regulation of appetite activated by its main ligand α-melanocyte-stimulating hormone (α-MSH) in both central and peripheral targets. α-MSH also binds to circulating immunoglobulins (Igs) but the functional significance of such immune complexes (ICs) in MC4R signaling in normal and pathological conditions of altered appetite has remained unknown. To address this question, we analyzed plasma levels, affinity kinetics, and binding epitopes of α-MSH-reactive IgG extracted from plasma samples of female patients with hyperphagic obesity, anorexia nervosa, bulimia nervosa, binge-eating disorder, and healthy controls. Ability of α-MSH/IgG IC to bind and activate human MC4R were studied in vitro and to influence feeding behavior in vivo in rodents. We found that α-MSH-reactive IgG were low in obese but increased in anorectic and bulimic patients and displayed different epitope and kinetics of IC formation. Importantly, while α-MSH/IgG IC from all subjects were binding and activating MC4R, the receptor binding affinity was decreased in obesity. Additionally, α-MSH/IgG IC had lower MC4R-mediated cAMP activation threshold as compared with α-MSH alone in all but not obese subjects. Furthermore, the cellular internalization rate of α-MSH/IgG IC by MC4R-expressing cells was decreased in obese but increased in patients with anorexia nervosa. Moreover, IgG from obese patients prevented central anorexigenic effect of α-MSH. These findings reveal that MC4R is physiologically activated by IC formed by α-MSH/IgG and that different levels and molecular properties of α-MSH-reactive IgG underlie biological activity of such IC relevant to altered appetite in obesity and eating disorders.
Autophagic flux blockage in alveolar epithelial cells is essential in silica nanoparticle-induced pulmonary fibrosis
Authors: Zhao Xinyuan , Wei Saisai , Li Zhijian , Lin Chen , Zhu Zhenfeng , Sun Desen , Bai Rongpan , Qian Jun , Gao Xiangwei , Chen Guangdi , Xu Zhengping 
Published: February 2019

AbstractSilica nanoparticles (SiNPs) have been reported to induce pulmonary fibrosis (PF) with an unknown mechanism. Recently, the activation of autophagy, a lysosome-dependent cell degradation pathway, by SiNPs has been identified in alveolar epithelial cells (AECs). However, the underlying mechanism and the relevance of SiNPs-induced autophagy to the development of PF remain elusive. Here, we report that autophagy dysfunction and subsequent apoptosis in AECs are involved in SiNPs-induced PF. SiNPs engulfed by AECs enhance autophagosome accumulation and apoptosis both in vivo and in vitro. Mechanically, SiNPs block autophagy flux through impairing lysosomal degradation via acidification inhibition. Lysosomal reacidification by cyclic-3′,5′-adenosine monophosphate (cAMP) significantly enhances autophagic degradation and attenuate apoptosis. Importantly, enhancement of autophagic degradation by rapamycin protects AECs from apoptosis and attenuates SiNPs-induced PF in the mouse model. Altogether, our data demonstrate a repressive effect of SiNPs on lysosomal acidification, contributing to the decreased autophagic degradation in AECs, thus leading to apoptosis and subsequent PF. These findings may provide an improved understanding of SiNPs-induced PF and molecular targets to antagonize it.
Hemi-methylated CpG sites connect Dnmt1-knockdown-induced and Tet1-induced DNA demethylation during somatic cell reprogramming
Authors: He Songwei , Wang Fuhui , Zhang Yixin , Chen Jinlong , Liang Lining , Li Yuan , Zhang Mengdan , Yang Xiao , Pang Hongshen , Li Yingying , Huang Xiaofen , Qin Dajiang , Pei Duanqing , Sun Hao , Zheng Hui 
Published: February 2019

AbstractThe relationship between active DNA demethylation induced by overexpressing Tet1 and passive DNA demethylation induced by suppressing Dnmt1 remains unclear. Here, we found that DNMT1 preferentially methylated, but TET1 preferentially demethylated, hemi-methylated CpG sites. These phenomena resulted in a significant overlap in the targets of these two types of DNA demethylation and the counteractions of Dnmt1 and Tet1 during somatic cell reprogramming. Since the hemi-methylated CpG sites generated during cell proliferation were enriched at core pluripotency loci, DNA demethylation induced by Tet1 or sh-RNA against Dnmt1 (sh-Dnmt1) was enriched in these loci, which, in combination with Yamanaka factors, led to the up-regulation of these genes and promoted somatic cell reprogramming. In addition, since sh-Dnmt1 induces DNA demethylation by impairing the further methylation of hemi-methylated CpG sites generated during cell proliferation, while Tet1 induced DNA demethylation by demethylating these hemi-methylated CpG sites, Tet1-induced DNA demethylation, compared with sh-Dnmt1-induced DNA demethylation, exhibited a higher ability to open the chromatin structure and up-regulate gene expression. Thus, Tet1-induced but not sh-Dnmt1-induced DNA demethylation led to the up-regulation of an additional set of genes that can promote the epithelial-mesenchymal transition and impair reprogramming. When vitamin C was used to further increase the demethylation ability of TET1 during reprogramming, Tet1 induced a larger up-regulation of these genes and significantly impaired reprogramming. Therefore, the current studies provide additional information regarding DNA demethylation during somatic cell reprogramming.
Contemporary climatic analogs for 540 North American urban areas in the late 21st century
Authors: Fitzpatrick Matthew C. , Dunn Robert R. 
Published: February 2019

AbstractA major challenge in articulating human dimensions of climate change lies in translating global climate forecasts into impact assessments that are intuitive to the public. Climate-analog mapping involves matching the expected future climate at a location (e.g., a person’s city of residence) with current climate of another, potentially familiar, location - thereby providing a more relatable, place-based assessment of climate change. For 540 North American urban areas, we used climate-analog mapping to identify the location that has a contemporary climate most similar to each urban area’s expected 2080’s climate. We show that climate of most urban areas will shift considerably and become either more akin to contemporary climates hundreds of kilometers away and mainly to the south or will have no modern equivalent. Combined with an interactive web application, we provide an intuitive means of raising public awareness of the implications of climate change for 250 million urban residents.
Whole-genome sequencing identifies ADGRG6 enhancer mutations and FRS2 duplications as angiogenesis-related drivers in bladder cancer
Authors: Wu Song , Ou Tong , Xing Nianzeng , Lu Jiang , Wan Shengqing , Wang Changxi , Zhang Xi , Yang Feiya , Huang Yi , Cai Zhiming 
Published: February 2019

AbstractBladder cancer is one of the most common and highly vascularized cancers. To better understand its genomic structure and underlying etiology, we conduct whole-genome and targeted sequencing in urothelial bladder carcinomas (UBCs, the most common type of bladder cancer). Recurrent mutations in noncoding regions affecting gene regulatory elements and structural variations (SVs) leading to gene disruptions are prevalent. Notably, we find recurrentADGRG6enhancer mutations andFRS2duplications which are associated with higher protein expression in the tumor and poor prognosis. Functional assays demonstrate that depletion ofADGRG6orFRS2expression in UBC cells compromise their abilities to recruit endothelial cells and induce tube formation. Moreover, pathway assessment reveals recurrent alterations in multiple angiogenesis-related genes. These results illustrate a multidimensional genomic landscape that highlights noncoding mutations and SVs in UBC tumorigenesis, and suggest ADGRG6 and FRS2 as novel pathological angiogenesis regulators that would facilitate vascular-targeted therapies for UBC.
Direct solution-phase synthesis of 1T’ WSe2 nanosheets
Authors: Sokolikova Maria S. , Sherrell Peter C. , Palczynski Pawel , Bemmer Victoria L. , Mattevi Cecilia 
Published: February 2019

AbstractCrystal phase control in layered transition metal dichalcogenides is central for exploiting their different electronic properties. Access to metastable crystal phases is limited as their direct synthesis is challenging, restricting the spectrum of reachable materials. Here, we demonstrate the solution phase synthesis of the metastable distorted octahedrally coordinated structure (1T’ phase) of WSe2 nanosheets. We design a kinetically-controlled regime of colloidal synthesis to enable the formation of the metastable phase. 1T’ WSe2 branched few-layered nanosheets are produced in high yield and in a reproducible and controlled manner. The 1T’ phase is fully convertible into the semiconducting 2H phase upon thermal annealing at 400 °C. The 1T’ WSe2 nanosheets demonstrate a metallic nature exhibited by an enhanced electrocatalytic activity for hydrogen evolution reaction as compared to the 2H WSe2 nanosheets and comparable to other 1T’ phases. This synthesis design can potentially be extended to different materials providing direct access of metastable phases.
Direct dehydrogenative alkyl Heck-couplings of vinylarenes with umpolung aldehydes catalyzed by nickel
Authors: Lv Leiyang , Zhu Dianhu , Li Chao-Jun 
Published: February 2019

AbstractAlkenes are fundamental functionalities in nature and highly useful intermediates in organic synthesis, medicinal chemistry and material sciences. Transition-metal-catalyzed Heck couplings with organic halides as electrophiles have been established as a powerful protocol for the synthesis of this valuable building block. However, the requirement of organic halides and the generation of stoichiometric hazardous halide wastes may cause significant sustainable concerns. The halide-free oxidative Heck alkenylations involving organometallics or arenes as the coupling partners provide a facile and alternative pathway. Nonetheless, stoichiometric amounts of extra oxidant are essential in most cases. Herein, we present a direct dehydrogenative alkyl Heck-coupling reaction under oxidant-free conditions, liberating hydrogen, nitrogen and water as the side products. Excellent regioselectivity is achieved via neighboring oxygen atom coordination. Broad substrate scope, great functional group (ketone, ester, phenol, free amine, amide etc) tolerance and modification of pharmaceutical candidates and biological molecules exemplified its generality and practicability.
Quantum magnetisms in uniform triangular lattices Li2AMo3O8 (A = In, Sc)
Authors: Iida Kazuki , Yoshida Hiroyuki , Okabe Hirotaka , Katayama Naoyuki , Ishii Yuto , Koda Akihiro , Inamura Yasuhiro , Murai Naoki , Ishikado Motoyuki , Kadono Ryosuke , Kajimoto Ryoichi 
Published: February 2019

AbstractMolecular based spin-1/2 triangular lattice systems such as LiZn2Mo3O8 have attracted research interest. Distortions, defects, and intersite disorder are suppressed in such molecular-based magnets, and intrinsic geometrical frustration gives rise to unconventional and unexpected ground states. Li2AMo3O8 (A = In or Sc) is such a compound where spin-1/2 Mo3O13 clusters in place of Mo ions form the uniform triangular lattice. Their ground states are different according to the A site. Li2InMo3O8 undergoes conventional 120° long-range magnetic order below TN = 12 K whereas isomorphic Li2ScMo3O8 exhibits no long-range magnetic order down to 0.5 K. Here, we report exotic magnetisms in Li2InMo3O8 and Li2ScMo3O8 investigated by muon spin rotation (μSR) and inelastic neutron scattering (INS) spectroscopies using polycrystalline samples. Li2InMo3O8 and Li2ScMo3O8 show completely different behaviors observed in both μSR and INS measurements, representing their different ground states. Li2InMo3O8 exhibits spin wave excitation which is quantitatively described by the nearest neighbor anisotropic Heisenberg model based on the 120° spin structure. In contrast, Li2ScMo3O8 undergoes short-range magnetic order below 4 K with quantum-spin-liquid-like magnetic fluctuations down to the base temperature. Origin of the different ground states is discussed in terms of anisotropies of crystal structures and magnetic interactions.
Cardiac fibroblast activation and hyaluronan synthesis in response to hyperglycemia and diet-induced insulin resistance
Authors: Gorski Daniel J. , Petz Anne , Reichert Christina , Twarock Sören , Grandoch Maria , Fischer Jens W. 
Published: February 2019

AbstractDiabetic patients are at a greater risk of heart failure due to diabetic cardiomyopathy and worsened outcome post-myocardial infarction. While the molecular mechanisms remain unclear, fibrosis and chronic inflammation are common characteristics of both conditions. Diabetes mellitus (types I and II) results in excessive hyaluronan (HA) deposition in vivo, and hyperglycemia stimulates HA synthesis for several cell types in vitro. HA-rich extracellular matrix contributes to fibrotic, hyperplastic and inflammatory disease progression. We hypothesized that excessive hyperglycemia-driven HA accumulation may contribute to pathological fibroblast activation and fibrotic remodelling in diabetic patients. Therefore, we analysed the impact of both hyperglycemia and diet-induced obesity and insulin resistance on HA matrix formation and cardiac fibroblast activation. Here we report that cardiac fibroblasts isolated from mice on a diabetogenic diet acquire pro-fibrotic gene expression without a concomitant increase in HA matrix deposition. Additionally, hyperglycemia alone does not stimulate HA synthesis or cardiac fibroblast activation in vitro, suggesting that the direct effect of hyperglycemia on fibroblasts is not the primary driver of fibrotic remodelling in cardiac diabetic maladaptation.
Ventricular-Subventricular Zone Contact by Glioblastoma is Not Associated with Molecular Signatures in Bulk Tumor Data
Authors: Mistry Akshitkumar M. , Wooten David J. , Davis L. Taylor , Mobley Bret C. , Quaranta Vito , Ihrie Rebecca A. 
Published: February 2019

AbstractWhether patients with glioblastoma that contacts the ventricular-subventricular zone stem cell niche (VSVZ + GBM) have a distinct survival profile from VSVZ − GBM patients independent of other known predictors or molecular profiles is unclear. Using multivariate Cox analysis to adjust survival for widely-accepted predictors, hazard ratios (HRs) for overall (OS) and progression free (PFS) survival between VSVZ + GBM and VSVZ − GBM patients were calculated in 170 single-institution patients and 254 patients included in both The Cancer Genome (TCGA) and Imaging (TCIA) atlases. An adjusted, multivariable analysis revealed that VSVZ contact was independently associated with decreased survival in both datasets. TCGA molecular data analyses revealed that VSVZ contact by GBM was independent of mutational, DNA methylation, gene expression, and protein expression signatures in the bulk tumor. Therefore, while survival of GBM patients is independently stratified by VSVZ contact, with VSVZ + GBM patients displaying a poor prognosis, the VSVZ + GBMs do not possess a distinct molecular signature at the bulk sample level. Focused examination of the interplay between the VSVZ microenvironment and subsets of GBM cells proximal to this region is warranted.
Catalytic preparation of levulinic acid from cellobiose via Brønsted-Lewis acidic ionic liquids functional catalysts
Authors: Liu Shiwei , Wang Kai , Yu Hailong , Li Binghan , Yu Shitao 
Published: February 2019

AbstractBrønsted-Lewis acidic ionic liquids (ILs) were applied to catalyze cellobiose to prepare levulinic acid (LA) in one pot under hydrothermal conditions. Under the optimum conditions, the highest LA yield of 67.51% was obtained when low [HO3S-(CH2)3-mim]Cl-FeCl3 (molar fraction of FeCl3 x = 0.60) was used. This indicated the Brønsted-Lewis acidic ILs played an active role in the conversion of cellobiose to LA. The catalytic mechanism of ILs had been established, disclosing that the Brønsted-Lewis acidic ILs had the catalytic synergistic effect originating from its double acid sites. During the reaction process, the Lewis acid sites improved the isomerization of glucose to fructose, then the Brønsted and Lewis acid sites simultaneously enhanced the dehydration of fructose to produce hydroxymethylfurfural (HMF), which was propitious to the synthesize LA with high yield. In addition, LA could be easily extracted by methyl isobutyl ketone (MIBK), and the ILs could retain its basic activity after 5 cycles. The solid residues were characterized using SEM, FT-IR and TG-DTG spectroscopy. It was the conclusion that a large amount of humins were produced during the cellobiose conversion process. In this reaction, the ILs not only overcomes the problems of the conventional catalyst, but also completes the reaction-separation integration and the recycling of the catalyst. This paper provided an important theoretical basis for the application of ILs in the field of biomass.
Distinct Subsets of Lateral Hypothalamic Neurotensin Neurons are Activated by Leptin or Dehydration
Authors: Brown Juliette A. , Wright Anna , Bugescu Raluca , Christensen Lyndsay , Olson David P. , Leinninger Gina M. 
Published: February 2019

AbstractThe lateral hypothalamic area (LHA) is essential for ingestive behavior but it remains unclear how LHA neurons coordinate feeding vs. drinking. Most LHA populations promote food and water consumption but LHA neurotensin (Nts) neurons preferentially induce water intake while suppressing feeding. We identified two molecularly and projection-specified subpopulations of LHA Nts neurons that are positioned to coordinate either feeding or drinking. One subpopulation co-expresses the long form of the leptin receptor (LepRb) and is activated by the anorectic hormone leptin (NtsLepRb neurons). A separate subpopulation lacks LepRb and is activated by dehydration (NtsDehy neurons). These molecularly distinct LHA Nts subpopulations also differ in connectivity: NtsLepRb neurons project to the ventral tegmental area and substantia nigra compacta but NtsDehy neurons do not. Intriguingly, the LHA Nts subpopulations cannot be discriminated via their classical neurotransmitter content, as we found that all LHA Nts neurons are GABAergic. Collectively, our data identify two molecularly- and projection-specified subpopulations of LHA Nts neurons that intercept either leptin or dehydration cues, and which conceivably could regulate feeding vs. drinking behavior. Selective regulation of these LHA Nts subpopulations might be useful to specialize treatment for ingestive disorders such as polydipsia or obesity.