bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pathogenesis of Anti–PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells
Authors: Kanie Keitaro , Bando Hironori , Iguchi Genzo , Muguruma Keiko , Matsumoto Ryusaku , Hidaka-Takeno Ryoko , Okimura Yasuhiko , Yamamoto Masaaki , Fujita Yasunori , Fukuoka Hidenori , Yoshida Kenichi , Suda Kentaro , Nishizawa Hitoshi , Ogawa Wataru , Takahashi Yutaka 
Published: August 2019

Abstract

Context
Anti–pituitary-specific transcriptional factor-1 (anti–PIT-1) antibody syndrome is characterized by acquired and specific deficiencies in growth hormone, prolactin, and thyroid-stimulating hormone. Although PIT-1–reactive cytotoxic T lymphocytes (CTLs) have been speculated to recognize anterior pituitary cells and to cause the injury in the pathogenesis of the syndrome, it remains unclear whether endogenous PIT-1 protein is processed through the proteolytic pathway and presented as an antigen on anterior pituitary cells.

Objective
To examine how PIT-1 protein is processed and whether its epitope is presented by major histocompatibility complex (MHC)/HLA class I on anterior pituitary cells.

Materials and Methods
Immunofluorescence staining and proximity ligation assay (PLA) were performed using anti–PIT-1 antibody and patients’ sera on PIT-1–expressing cell line GH3 cells and human induced pluripotent stem cell (iPSC)-derived pituitary tissues.

Results
PIT-1 was colocalized with MHC class I molecules, calnexin, and GM130 in the cytosol. PLA results showed that PIT-1 epitope was presented by MHC/HLA class I molecules on the cell surface of GH3 cells and iPSC-derived pituitary cells. The number of PIT-1/HLA complexes on the cell surface of pituitary cells in the patient was comparable with that in the control subject.

Conclusions
Our data indicate that PIT-1 protein is processed in the antigen presentation pathway and that its epitopes are presented by in MHC/HLA class I on anterior pituitary cells, supporting the hypothesis that PIT-1–reactive CTLs caused the cell-specific damage. It is also suggested that number of epitope presentation was not associated with the pathogenesis of anti–PIT-1 antibody syndrome.
Multifocus Fluorescence Correlation Spectroscopy with Spatially Separated Excitation Beams
Authors: Otosu Takuhiro , Ishii Kunihiko , Tahara Tahei 
Published: August 2019

Abstract
We report on a new multifocus optical system developed for fluorescence correlation spectroscopy (FCS). This system utilizes a beamsplitter array to spatially separate excitation beams. Fluorescence from each spot is individually detected with a single-photon avalanche photodiode (SPAD) through a fiber bundle. The combined use of beamsplitters and multiple SPADs is beneficial to give a higher detection uniformity among focal spots than the existing multifocus FCS systems. Applications of this setup show that the variation of the optical parameters among seven focal spots are as small as ∼5% and prove its high performance in FCS measurements. High efficiency of this system is also demonstrated in application to an advanced FCS method, two-dimensional fluorescence lifetime correlation spectroscopy. Along with the results of these applications, we describe a method for proper correction of the crosstalk effect in the multifocus setup.
Stimulating the Search Activity of Primary Schoolchildren in the Process of Solving Educational Problems
Authors: Аксючиц Светлана , Aksyuchic Svetlana 
Published: August 2019

The article raises the problem of the formation of the search activity of younger schoolchildren and the formation of their subjective position in learning. The essence of the concept of “educational task” is revealed as a component of educational activity. The structure of the lesson is described, in the process of which the skills of younger schoolchildren to solve educational problems that are aimed at mastering generalized methods of objective actions are formed. This provides the opportunity for students to master the system of scientific concepts that allows selfdevelopment and self-learning. A concrete example of solving the educational problem in the lesson of the Russian language in the second grade
is given.
Health problems of individuals with autism spectrum disorder
Authors: Yılmaz Medine , Sari Hatice Yildirim , Coşansu Gülhan Kuzu , Nahcivan Nursen , Aktaş Betül , Tiraki Zeliha , Dogruyol Devrim 
Published: August 2019

Aim: The purpose of this study is to determine the health needs of individuals with autism spectrum disorder (ASD) using the Problem Classification Scheme (PCS), one of the main components of the Omaha System. The data obtained are expected to guide health programmes focusing on the needs of individuals with ASD. Method: This descriptive study was conducted at a special education implementation centre providing daytime education for individuals with ASD. Data obtained by the institutional nurse during the daily diagnosis and examination procedures of the ambulatory treatment unit in the centre were defined by another researcher according to the PCS forms. Routine health care was provided for all the individuals whose condition was diagnosed. Results: The 10 most frequently documented problems were personal care (83.78%), mental health (62.84%), speech and language (61.49%), social contact (50%), interpersonal relationship (42.57%), cognition (32.43%), income (28.38%), medication regimen (24.32 %), nutrition (22.30%) and neglect (18.92%). Conclusion: The results indicate that it is useful to use the Omaha System in determining the health needs of an individual with autism holistically, and in assessing and evaluating the individual's care, as it provides a standardised language. Impact statements: The Omaha PCS System can be used by nurses in the holistic diagnosis of the health status of individuals with ASD.
Using ecosystem approach indicators for assessing the ecological status of reef fisheries management in a marine protected area
Authors: YULIANA ERNIK , BOER MENNOFATRIA , FAHRUDIN ACHMAD , KAMAL MOHAMMAD MUKHLIS , PARDEDE SHINTA TRILESTARI 
Published: August 2019

Abstract. Yuliana E, Boer M, Fahrudin A, Kamal MM, Pardede ST. 2019. Using ecosystem approach indicators for assessing the ecological status of reef fisheries management in a marine protected area. Biodiversitas 20: 1802-1810. The purpose of this study was to assess the status of reef fisheries management in a marine protected area base on ecosystem approach indicators and to determine the tactical decisions required to improve management. The study site was in Karimunjawa National Park (KNP) Jepara, Central Java, Indonesia. Data were collected using survey and observation methods and included both primary and secondary data. Coral fish, which were the object of the study was Caesio cuning, Caesio caerulaurea, Plectropomus oligacanthus, and Parupeneus barberinus. Determination of the status of each ecosystem approach indicator was conducted using the flag model. Tactical decisions were necessary to improve the status of management indicators. The results indicated that the ecological status of reef fisheries management of KNP is at a moderate level with an achievement value of 62.75% Tactical decisions are necessary to push the ten indicators to achieve a better level. Three indicators still received a poor status, and the remaining (seven indicators) were within the moderate status. Therefore, the three indicators that need to be improved quickly are fish abundance, fishing gear modification, and fishing capacity.
Emperor Menilek II and the Art of Manuscript Illumination: The Politics of Representation in Nineteenth-Century Ethiopia
Authors: Jenkins Earnestine 
Published: August 2019

Abstract
Menelik II was both King of Shewa (1865–1888) and Emperor of Ethiopia (1889–1912). In the late nineteenth century he was confronted with issues unique to the era of European colonization in Africa. This paper examines Menelik II’s use of the visual arts in expressing and communicating ideas about royal authority and power. I examine several illuminated manuscripts commissioned by Menelik II. The books document how Menelik II borrowed and adapted objects and symbols from Ethiopia’s ancient past as a Christian power. Menelik realized that his image as an African ruler who retained his independence was unique within the contexts of late nineteenth-century Africa, wherein the entire continent was colonized except for Liberia and Ethiopia. Menelik II used the traditional Christian art of manuscript illumination in Ethiopia to create visual images that symbolically reinforced his authority as both a Christian and an African sovereign.
The Popular Image of Därgue as Reflected in Amharic Folk Poetry (1974–91)
Authors: Workneh Melakneh Mengistu 
Published: August 2019

Abstract
The popular image of governments that rise and demise is determined by their integrity, vision, and responsiveness to the aspirations of the populace. The resultant social phenomenon, known as popular image or perception, could thus be viewed either as an expression of conformism or nonconformism with the status quo. One of the means by which the public externalizes its outlook is folk literature, which is permeated with the tragedy and comedy of the subject people. This study was designed to determine the popular image of the military government of Ethiopia (1974–91) as reflected in contemporary Amharic folk poetry, which revealed the duality of public perception. On the one hand, the selected couplets are found to be critical of the status quo, which perpetuates negative images. On the other hand, the Därgue1 is perceived as a nationalist government credited with protecting the national interest and the territorial integrity of the country irrespective of its inadvertent malpractices. At any rate, politically motivated propaganda against the military government of Ethiopia seems to have backfired, because most of the couplets evoke nostalgia for the past.
THE EFFECTIVENESS OF INFRASTRUCTURE DEVELOPMENT FOR WASTEWATER SERVICE IN DECENTRALIZED INDONESIA: CASE OF BANDUNG MUNICIPALITY
Authors: Waskitawati Diny , Widianingsih Ida , Gunawan Budhi 
Published: August 2019

This paper discusses the effectiveness of infrastructure development for wastewater service in decentralized Indonesia, particularly in Bandung municipality as one of the biggest cities in the country where sanitation problems persist. Although wastewater service coverage in Bandung municipality shows a gradual improvement from 2010 to 2016, the research identifies the ineffectiveness of wastewater infrastructure development. Qualitative analyses revealed that the wastewater infrastructure development in Bandung municipality funds came from two different sources (local budget and internal donor support). We would argue that in the case of wastewater infrastructure development, the same amount of money shares different processes and results. Compared to AusAID water and sanitation program, in term of value of money, the one designed and managed by the Municipality Water Supply Agency (PDAMs) is less effective. It is concluded the newly developed decentralization laws in Indonesia could not ensure the effectiveness of public service delivery due to the absence of sound governance at local level.
Homeoprotein Neuroprotection of Embryonic Neuronal Cells
Authors: Vargas Abonce Stephanie E. , Leboeuf Mélanie , Prochiantz Alain , Moya Kenneth L. 
Published: August 2019

AbstractMost homeoprotein transcription factors have a highly conserved internalization domain used in intercellular transfer. Internalization of homeoproteins ENGRAILED1 or ENGRAILED2 promotes the survival of adult dopaminergic cells, whereas that of OTX2 protects adult retinal ganglion cells. Here we characterize thein vitroneuroprotective activity of several homeoproteins in response to H2O2. Protection is observed with ENGRAILED1, ENGRAILED2, OTX2, GBX2, and LHX9 on midbrain and striatal embryonic neurons, whereas cell-permeable c-MYC shows no protective effects. Therefore, five homeoproteins belonging to three different classes (ANTENNAPEDIA, PAIRED, and LIM) share the ability to protect embryonic neurons from midbrain and striatum. Because midbrain and striatal neurons do not express the same repertoire of the four proteins, a lack of neuronal specificity together with a general protective activity can be proposed. Interestingly, hEN1 and GBX2 provided protection to primary midbrain astrocytes but not to non-neural cells, including mouse embryo fibroblasts, macrophages or HeLa cells. For the four proteins, protection against cell death correlated with a reduction in the number of H2O2-induced DNA break foci in midbrain and striatal neurons. In conclusion, within the limit of the number of cell types and homeoproteins tested, homeoprotein protection against oxidative stress-induced DNA breaks and death is specific to neurons and astrocytes but shows no homeoprotein or neuronal type specificity.
Adaptations during Maturation in an Identified Honeybee Interneuron Responsive to Waggle Dance Vibration Signals
Authors: Kumaraswamy Ajayrama , Ai Hiroyuki , Kai Kazuki , Ikeno Hidetoshi , Wachtler Thomas 
Published: August 2019

AbstractHoneybees are social insects, and individual bees take on different social roles as they mature, performing a multitude of tasks that involve multi-modal sensory integration. Several activities vital for foraging, like flight and waggle dance communication, involve sensing air vibrations through their antennae. We investigated changes in the identified vibration-sensitive interneuron DL-Int-1 in the honeybeeApis melliferaduring maturation by comparing properties of neurons from newly emerged adult and forager honeybees. Although comparison of morphological reconstructions of the neurons revealed no significant changes in gross dendritic features, consistent and region-dependent changes were found in dendritic density. Comparison of electrophysiological properties showed an increase in the firing rate differences between stimulus and nonstimulus periods in foragers compared with newly emerged adult bees. The observed differences in neurons of foragers compared with newly emerged adult honeybees suggest refined connectivity, improved signal propagation, and enhancement of response features possibly important for the network processing of air vibration signals relevant for the waggle dance communication of honeybees.
Association of GDNF and CNTNAP2 gene variants with gambling
Authors: Das Arundhuti , Pagliaroli Luca , Vereczkei Andrea , Kotyuk Eszter , Langstieh Banrida , Demetrovics Zsolt , Barta Csaba 
Published: August 2019

Background and aims
Some form of gambling can be observed in nearly every society, as the gratification felt upon winning in uncertain conditions is universal. A culturally distinct form of gambling, associated with a traditional sporting event of archery known as “teer,” is innate to the province of Meghalaya, India. The objective of this study was to find genetic variants underlying this unique form of behavioral addiction. To better understand game-based gambling, we studied genetic variants related to dopaminergic pathways and other genes previously linked to various psychological disorders.

Methods
This study was carried out on a sample of 196 Indo-Aryan adults from Shillong, Meghalaya. Genotyping of glial cell line-derived neurotrophic factor (GDNF) polymorphisms was carried out using real-time PCR. We further investigated 32 single nucleotide polymorphisms located in the 3′ UTR of additional genes of interest using an OpenArray® real-time PCR platform.

Results
Case–control analysis revealed a significant association between GDNF variant rs2973033 (p = .00864, χ2 = 13.132, df = 2) and contactin-associated protein-like 2 (CNTNAP2) variant rs2530311 (p = .0448, χ2 = 13.132, df = 2) with gambling.

Discussion and conclusions
Association of the GDNF gene with gambling could be attributed to its involvement in the development and survival of dopaminergic neurons. Our result is in good agreement with previous data indicating the role of GDNF in certain substance addictions. Several rare variants in the CNTNAP2 gene were also implicated in alcohol addiction in a previous study. This pilot study provides further support for the role of GDNF and CNTNAP2 in addiction behaviors.
Ways to Reduce Power Losses in Mining Power Supply Lines
Authors: Antsiferov N. S. 
Published: August 2019

Electric energy is the most common and universal form of energy. It can be produced in large quantities near energy sources, transmitted to large distances, easily distributed between consumers and converted into mechanical, thermal, and light energy. Ensuring reliable and economical quality electric energy supply to mining enterprises with the optimal use of energy resources is one of the most important tasks facing the country's energy sector. In the Russian Federation, high degree of concentration of generating capacities at power plants has been achieved. The main capacities are concentrated in power plants with installed capacity of more than 1 million kW. High importance of the electric power industry is determined by the infrastructural nature of the industry and the direct relationship between the country's economic growth and the level of its development effectiveness. One of the most pressing problems of modern electricity supply is the problem of ensuring the quality of electric energy. The main reason for the deterioration in the electricity quality is widespread non-linear loads that create non-sinusoidal currents and voltage unbalance during their operation. The voltage unbalance is most often caused by the presence of an unbalanced load. Unbalanced load currents flowing through the elements of the power supply system cause unbalanced voltage losses in them. This results in appearance of unbalanced voltages on the terminals of electric apparatus. The voltage deviations at electric apparatus overdriven phase may exceed the normally acceptable values, while the voltage deviations at electric apparatus other phases may remain within the normal limits. In addition to the deterioration of the voltage mode at electric apparatus under the unbalanced voltage conditions, the operating conditions of both the electric apparatus and all the network elements are significantly worsened, and the reliability of the electrical equipment and the power supply system as a whole decreased. The paper considers the issues of reducing the voltage losses in power supply networks by improving quality of electric power.
Effect of Operating Factors on Reliability of Stoping Complexes and Assessment of Reliability of their Elements
Authors: Troinich V. A. , Dubovsky A. A. , Vysotskaya N. A. 
Published: August 2019

For analyzing the dependence of face equipment failure on its length, two groups of elements are commonly considered. The first group includes all elements of shearer-loaders: conveyor drives, elements of pumping stations of powered supports, supports of face junctions with strikes and others. The second group includes all elements of powered support sections, linear sections of pan lines and scrapers of face scraper conveyors, electric cables of shearer-loaders, main pipelines of powered supports, etc. It is noted that the constancy of number of the first group elements linear variability of number of the second group elements do not uniquely determine the constancy or variability of the failure factor of the aggregate of the same type elements of the first or the second groups [1]. The plot of mean-time-between-failures (MTBF) of SL-500S stoping complex as function of face length is presented. Besides, the curve of the face (complex) length-dependence of average recovery) time (after failure of the SL-500S stoping complex time is shown. Analyzing the dependence of availability factor of stoping complexes on the face length showed that the length of stoping complexes is not a factor determining decrease in the MTBF and increase in the average recovery time. The plot of recovery time (after failure) of the SL-500S stoping complex as function of face length is shown. A formula is presented for assessing the cumulative effect, on the MTBF of SL500S stoping complex, of its length and potash ore cuttability. The plot of correlation between the MTBF of SL500S stoping complex and the face length/the potash ore cuttability is presented, which demonstrates that the complex length followed by the thickness of the extracted layer produce the greatest effect on the MTBF. The plot of the number of failures per day as a function of the maintenance factor of the SL-500C shearer-loader is presented. The plot demonstrates that the average number of failures of the SL-500C shearer-loader per day reaches a minimum and practically stabilize at values of the maintenance factor of 0.9–1.0, which correspond to three-shift production with one 6-hour maintenance shift per day.
Primary infective endocarditis with isolated involvement of the pulmonary valve not associated with drug addiction
Authors: Chipigina N. S. , Karpova N. Yu. , Tulinov M. M. , Golovko E. V. , Goloukhova L. M. , Kornienko V. S. , Kostin A. Yu. , Barsegyan V. A. 
Published: August 2019

Objective: to describe a rare case of infective endocarditis (IE) with isolated localization in the pulmonary valve (PV).Materials and methods. We observed primary IE with isolated localization in the PV in a 27-year-old female patient without risk factors of right-side IE.Results. The disease was caused by Streptococcus gordonii and proceeded acutely with typical signs of right-side IE: fever above 38 °С, chills, clinical picture of bilateral septic embolic abscess pneumonia, as well as secondary anemia, secondary thrombocytopenia, and glomerulonephritis. Echocardiography showed large vegetations in the PV prolapsing in the right ventricle and pulmonary artery.Conclusion. IE with localization in the PV should be suspected in patients with fever and clinical picture of septic embolic pneumonia in absence of other embolic situations.
A clinical case of systemic amyloidosis in a cardiologists’s practice
Authors: Okorokov V. G. , Evsina O. V. , Fomina V. A. , Ivanova G. O. , Soldatov E. S. , Dianov D. V. , Tkachenko K. A. , Ganyuta A. V. 
Published: August 2019

The aimof the work was to study the clinical case of systemic amyloidosis.Materials and methods. Patient Ch., 63 уears old, have admitted to the regional cardiological dispensary with complaints of short stabbing pains in the heart, without reaction to nitroglycerin, interruptions in the work of the heart, mainly at night, shortness of breath with little exertion, weakness, swelling of the legs and feet in September 2018. Sick from April 2016, when was dyspnea on exertion. The high level of creatinine, normochromic anemia have detected. In June 2016 chronic pyelonephritis was diagnosed. In August 2018, a right-sided hydrothorax was diagnosed, a pleural puncture was performed.Results. Laboratory and instrumental research methods were performed. Based on anamnesis data (normochromic anemia, proteinuria, increased creatinine in the blood), clinical picture, data of instrumental studies (restrictive cardiomyopathy, bilateral hydrothorax) clinical diagnosis was made: systemic amyloidosis the kidneys and heart with chronic kidney disease C5 and chronic heart failure. Histological evidence of amyloid and determination of the variant of amyloidosis by immunohistochemical method was recommended. But the patient died before the study. The histological verification of the diagnosis was carried out at the autopsy.Conclusion.This clinical observation is an example of late diagnosis of systemic amyloidosis and postmortal verification of the diagnosis, which is associated with the nonspecific symptoms and the rapidly progressing course of the disease. This case is interesting because amyloidosis proceeded under the guise of other diseases, quickly led to the development of severe heart and renal failure and to the death of the patient before verifying the disease.