bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential Oil Compositions and Antimicrobial Activities of Syzygium fluviatile (Hemsl.) Merr. & L.M.Perry: A Comparative Study on Collection Regions
Authors: Dai Do Ngoc , Xuyen Do Thi , Huong Le Thi , Anh Nguyen Thi Hoang , Ha Nguyen Xuan , The Son Ninh 
Published: October 2024

Objective Essential oils extracted from Syzygium species are known for various pharmacological purposes. Syzygium fluviatile (Hemsl.) Merr. & L.M.Perry is a flowering plant found in China and Vietnam. The current study aims to offer a comparison of chemical compositions in essential oils of S. fluviatile fruits and leaves, collected from five regions of Vietnam. The obtained oils were also taken into antimicrobial consideration, which was further aided by in silico approaches. Methods Phytochemical analysis of essential oils was carried out using GC-FID/MS (gas chromatography-flame ionization detection/mass spectrometry) analysis. An antimicrobial assay was performed using broth micro-dilution for in vitro screening. In silico considerations are mainly based on docking studies and toxicity assessments using the AutoDock Vina v1.2.3 program and the ProTox 3.0 web server, respectively. Results Hydro-distillation of S. fluviatile fresh fruits and leaves can lead to the production of yellow essential oil with yields of 0.21–0.32% v/w. In general, the obtained oils were dominated by monoterpene and sesquiterpene derivatives, as well as ( E)-caryophyllene (8.40-47.12%) being the principal compound. The oil samples showed strong antimicrobial activity against the Gram (+) bacteria Enterococcus faecalis ATCC51299, Staphylococcus aureus ATCC29213, and Bacillus cereus ATCC11778, and the yeast Candida albicans ATCC 60193 with the MIC and IC50 values 16–64 µg/mL and 5.12–24.68 µg/mL. Docking results indicated that ( E)-caryophyllene exhibited binding affinities from −6.728 kcal/mol to −5.729 kcal/mol with important amino acid residues in the DNA gyrase, PBP3, and SAP2 targets. The toxicity profile of ( E)-caryophyllene is also discussed. Conclusion The isolation of ( E)-caryophyllene from Vietnamese Syzygium essential oils as a purified compound is necessary. In vivo antimicrobial studies and molecular mechanisms of action are needed.
Injuries, Risk Factors, and Prevention Strategies in Bicycle Motocross (BMX): A Scoping Review
Authors: Rockliff Claire , Pulsifer Karen , Gupta Srijal , Jewell Carley B. , Black Amanda M. 
Published: October 2024

Context: Bicycle motocross (BMX) has become increasingly popular since its inclusion in the 2008 Olympics, but it has some of the highest injury rates (IRs) in multisport studies. To support planning for tailored primary prevention, understanding gaps in BMX injury prevention is crucial. Objective: To examine the evidence on injury incidence, prevalence, risk factors, prevention strategies, and prevention implementation in BMX. Data Sources: Ovid MEDLINE, Embase, APA PsycInfo, CINAHL, and SPORTDiscus were searched systematically in June 2023. Study Selection: Articles including BMX and any injury as the main topic or subtopic were searched across multiple databases. Study Design: A scoping review was designed following the PRISMA Extension for Scoping Reviews (PRISMA-ScR). Level of Evidence: Level 4. Data Extraction: BMX injury incidences, prevalence, risk factors, prevention strategies, and prevention implementation were extracted. Two reviewers screened all studies and extracted data independently. Results: Of the 1856 articles screened, 37 met inclusion criteria. Most studies used injury surveillance at elite competitions or emergency departments, and common injuries were contusions, lacerations, and fractures. IRs provided were based primarily on elite competition and were heterogeneous (eg, 2016 Olympics: 37.5 per 100 athletes; 2007 BMX World Championship: 11.7 per 100 athletes; 1989 BMX Euro Championship: 6.6 per 100 athletes). Only 1 study stratified IRs by BMX discipline (BMX freestyle: IR, 22.2 injuries per 100 athletes; BMX racing: IR, 27.1 per 100 athletes). Few prevention strategies have been evaluated, but reducing the number of riders per race could be helpful. Conclusion: Most BMX studies do not use recommended injury surveillance methodology. Studies based on emergency department data may underestimate minor injuries and do not adequately measure BMX exposures. Rigorous community-based prospective studies examining IRs for both BMX racing and freestyle, risk factors, and prevention strategies are needed to inform widespread evidence-based prevention strategies.
Fluctuation of Bone Marrow Lesions and Inflammatory MRI Markers over 2 Years and Concurrent Associations with Quantitative Cartilage Loss
Authors: Roemer Frank W. , Jansen Mylène P. , Maschek Susanne , Mastbergen Simon C. , Marijnissen Anne-Karien , Wisser Anna , Heiss Rafael , Weinans Harrie H. , Blanco Francisco J. , Berenbaum Francis , Kloppenburg Margreet , Haugen Ida K. , Eckstein Felix , Hunter David J. , Guermazi Ali , Wirth Wolfgang 
Published: October 2024

Objective To assess whether change of semiquantitatively magnetic resonance imaging (MRI)-defined bone marrow lesions (BMLs) and inflammatory markers is associated with change in quantitatively-assessed cartilage loss in the femorotibial joint (FTJ) in knees with radiographic osteoarthritis (OA) over 24 months. Design Participants were included from the IMI-APPROACH and the Osteoarthritis Initiative FNIH studies. Semiquantitative MRI assessment was performed for BMLs, Hoffa- and effusion-synovitis. Quantitative cartilage thickness measurements were performed manually. Definitions of change included number of subregions with BMLs, change in sum and change in maximum increase in size. Change in Hoffa-synovitis and effusion-synovitis was categorized in addition. Between-group comparisons regarding cartilage loss in the FTJ, medial and lateral compartments were performed using analysis of variance (ANOVA). Results A total of 629 participants were included. Knees without any BMLs at baseline (BL) and follow-up (FU) had significantly less cartilage loss compared to the other subgroups. Change in both directions in the sum score of BMLs was associated with increased rates of cartilage loss. Maximum increase in size of BMLs was associated with increased rates of cartilage loss (FTJ increase by 2 grades −0.183 mm, 95% CI [−0.335, −0.031], by 3 grades −0.306 mm, [−0.511, −0.101]). Worsening of Hoffa-synovitis was associated with increased rates of cartilage loss. Conclusion Knees without BMLs at BL and FU showed lowest rates of cartilage loss. Knees with an increase in BML size showed increased rates of concurrent cartilage loss. Approaches with the aim to inhibit BML development, avoidance of increase in size and avoidance of Hoffa-synovitis worsening may have beneficial effects on cartilage loss.
Reevaluating Informed Consent: Integrating Shared Decision-Making into Spinal Surgery for Better Patient Outcomes
Authors: Wang Jeffrey N. , Elhakeem Mohamed A. , Mesimer Matthew J. , Mastrokostas Paul G. , Ahmad Salman , Reed Tim , Klein Brandon , Bartlett Lucas E. , Bitterman Adam D. , Megas Andrew 
Published: October 2024

Study Design Narrative review. Objectives The objectives of this study were to answer the following questions: (1) What is the quality of informed consent in spine surgery, including both neurosurgery and orthopaedic spine surgery? (2) What limitations impede the ability of surgeons to engage in effective shared decision-making (SDM) and obtain adequate informed consent? (3) What strategies and solutions may improve the quality of informed consent and SDM? (4) What factors decrease the incidence of litigation in spine surgery? Methods N/A. Results SDM is a collaborative process where patients are involved in their treatment choices through open communication about risks, alternatives, and postoperative expectations. Informed consent is a vital component of this process, ensuring that patients are fully informed and empowered to make decisions based on their values and preferences. This review highlights the current state of informed consent within the context of SDM in spine surgery and explores how enhancing this process can improve patient outcomes, reduce dissatisfaction, and decrease litigation. By emphasizing patient autonomy and improving the quality of risk communication, SDM fosters better physician-patient relationships and more positive clinical outcomes. Conclusions Orthopaedic surgery and neurosurgery are highly litigated specialties, with failure to obtain informed consent frequently cited in lawsuits. These legal challenges are costly and time-consuming for both physicians and patients. Integrating SDM into the informed consent process can help mitigate these issues, leading to improved patient satisfaction and fewer legal disputes.
The Effects of Coffee/Caffeine in Patients with Multiple Sclerosis; A Systematic Review
Authors: Salekzamani Shabnam , Baharomid Saman , Pakkhesal Sina , Balafkandeh Maryam , Gholipour-Khalili Elnaz , Talebi Mahnaz , Sanaie Sarvin , Naseri Amirreza 
Published: October 2024

Background Multiple sclerosis (MS) is a demyelinating disease of the central nervous system. Caffeine, as the most widely consumed psychoactive substance, has been suggested to have potential effects on the clinical course and disability levels of MS patients. This study aimed to review the current evidence on the effects of coffee/caffeine in patients with MS. Methods This study followed the Joanna Briggs Institute (JBI) Manual for Evidence Synthesis and PRISMA 2020 statement. Clinical evidence regarding the effects of caffeine/coffee in MS patients was considered. A systematic search was performed in PubMed, Scopus, Web of Science, and Embase in October 2023, and updated via handsearching in March 2024. JBI's critical appraisal tools were utilized to scrutinize the risk of bias. Results Out of 297 screened records, eight studies were eventually found to meet our inclusion criteria. The sample size of the studies varied between 12 and 1372 and the study designs were retrospective cohort, RCT, single-blind crossover trial, single-arm pilot study (each one study), and cross-sectional (four studies). No significant association between the level of disability and coffee/caffeine intake has been reported, although it was reported to be associated with cognitive improvements. Discussion Evidence indicates an association between coffee/caffeine consumption, and improved cognitive outcomes in patients with MS, while there is no considerable relationship with the disease disability. Considering the limitations of the evidence, such as the small number of studies, and great diversity in study designs, the findings of this study should translate to clinical practice with caution.
CHRDL1 inhibits OSCC metastasis via MAPK signaling-mediated inhibition of MED29
Authors: Huang Songkai , Zhang Junwei , Qiao Yu , Pathak Janak Lal , Zou Rui , Piao ZhengGuo , Xie ShiMin , Liang Jun , Ouyang Kexiong 
Published: October 2024

Abstract
Background
CHRDL1 belongs to a novel class of mRNA molecules. Nonetheless, the specific biological functions and underlying mechanisms of CHRDL1 in oral squamous cell carcinoma (OSCC) remain largely unexplored.

Methods
RT-qPCR and immunohistochemical staining were employed to assess the mRNA and protein expression levels of the MED29 gene in clinical samples of OSCC. Additionally, RT-qPCR and Western Blot analyses were conducted to investigate the mRNA and protein expression levels of the MED29 gene specifically in OSCC. The impact of MED29 on epithelial-mesenchymal transition (EMT), invasion, and migration of OSCC was evaluated through scratch assay, transwell assay, and immunofluorescence staining. Furthermore, wound healing assay and Transwell assay were utilized to examine whether CHRDL1 influences the malignant behavior of OSCC by modulating MED29 in vitro. The regulatory role of CHRDL1 on MED29 was further elucidated in vivo through a tail vein lung metastasis model in nude mice.

Results
MED29 expression was elevated in tumor tissues of OSCC patients compared with adjacent cancer tissues. Moreover, in CAL27 and SCC25 cell lines, MED29 was upregulated and associated with increased cell migration and invasion abilities. Overexpression of MED29 facilitated EMT in OSCC cell lines, whereas knockdown of MED29 impeded EMT, resulting in diminished cell migration and invasion capacities. CHRDL1 exerted inhibitory effects on the expression of MED29, thereby suppressing EMT progression and consequently restraining the invasion and migration of OSCC cells. Furthermore, CHRDL1 mediated the inhibition of migration of OSCC cell lines to the OSCC through its regulation of MED29.

Conclusions
MED29 facilitated the epithelial-mesenchymal transition process in OSCC, thereby promoting migration and invasion. On the other hand, CHRDL1 exerted inhibitory effects on the invasion and metastasis of OSCC by suppressing MED29 through the inhibition of the MAPK signaling pathway.
Assessing personalized molecular portraits underlying endothelial-to-mesenchymal transition within pulmonary arterial hypertension
Authors: Wu Ruhao , Zhang Ge , Guo Mingzhou , Li Yue , Qin Lu , Jiang Tianci , Li Pengfei , Wang Yu , Wang Ke , Liu Yize , He Zhiqiu , Cheng Zhe 
Published: October 2024

AbstractPulmonary arterial hypertension (PAH) is a progressive and rapidly fatal disease with an intricate etiology. Identifying biomarkers for early PAH lesions based on the exploration of subtle biological processes is significant for timely diagnosis and treatment. In the present study, nine distinct cell populations identified based on gene expression profiles revealed high heterogeneity in cell composition ratio, biological function, distribution preference, and communication patterns in PAH. Notably, compared to other cells, endothelial cells (ECs) showed prominent variation in multiple perspectives. Further analysis demonstrated the endothelial-to-mesenchymal transition (EndMT) in ECs and identified a subgroup exhibiting a contrasting phenotype. Based on these findings, a machine-learning integrated program consisting of nine learners was developed to create a PAH Endothelial-to-mesenchymal transition Signature (PETS). This study identified cell populations underlying EndMT and furnished a potential tool that might be valuable for PAH diagnosis and new precise therapies.
Optimizing yield and water productivity in summer mung bean (Vigna radiata L.) through crop residue management and irrigation strategies
Authors: Tripathi Saurabh , Kaur Anureet , Brar Ajmer Singh , Sekhon Karamjit Singh , Singh Sukhpreet , Malik Anurag , Kisi Ozgur 
Published: October 2024

AbstractA multi-season research trial entitled ‘crop residue management effects on yield and water productivity of summer mung bean (Vigna radiata L.) under different irrigation regimes in Indian Punjab’ was conducted at Punjab Agricultural University (PAU), Regional Research Station (RRS), Bathinda, during rabi 2020 and 2021. The field experiment was conducted in a split-plot layout with nine treatment combinations and replicated thrice. The treatments consisted of T1 (no wheat residue along with tillage), T2 (leftover wheat residue with zero tillage), and T3 (incorporated wheat residue along with tillage) in main plots and irrigation regimes viz., I1 (vegetative growth and flowering stage), I2 (vegetative growth, flowering, and pod filling stage) and I3 (vegetative growth, flowering, pod formation and pod filling stage) in sub-plots, respectively. The growth and yield attributing characters were significantly higher under T3 than T1 but statistically at par with T2 during both years. An increase of 24.1% and 19.0% in grain yield was found in residue incorporation (T3) and residue retention (T2) over residue removal (T1), respectively. Maximum crop and irrigation water productivity was observed under T3 due to reduced water use and increased yield. Among the irrigation regimes, the I3 recorded significantly higher grain yield (0.70 and 0.79 t ha− 1) than I1. It was at par with I2 during both years due to higher irrigation frequency at the pod formation and pod filling stage. Crop water productivity (CWP) was higher under I3, whereas irrigation water productivity (IWP) was higher under I1 during both years. Additional irrigation at the pod-filling stage increased the grain yield by 36.5%, and two additional irrigations at the pod-formation and pod-filling stage further increased yield by 46.2% compared to only two irrigations at the vegetative and flowering stages. The treatment combinations of T2I2 and T3I2 outperformed T1I3 in terms of growth and yield attributing characters viz. plant height, dry matter accumulation (DMA), leaf area index (LAI), pods plant− 1, seeds pod− 1, and 1000-seed weight, which resulted in higher grain yield in these treatment combinations over T1I3. Applying crop residue can help minimize water use and increase crop water productivity. So, retaining crop residue in summer mung bean resulted in saving irrigation water due to lesser evapotranspiration from the soil surface.
Hybridization has localized effect on genetic variation in closely related pine species
Authors: Szczepański Sebastian , Łabiszak Bartosz , Lasek Martyna , Wachowiak Witold 
Published: October 2024

Abstract
Background
Hybridization is a known phenomenon in nature but its genetic impact on populations of parental species remains less understood. We investigated the evolutionary consequences of the interspecific gene flow in several contact zones of closely related pine species. Using a set of genetic markers from both nuclear and organellar genomes, we analyzed four hybrid zones (384 individuals) and a large panel of reference allopatric populations of parental taxa (2104 individuals from 96 stands).

Results
We observed reduced genetic diversity in maternally transmitted mitochondrial genomes of pure pine species and hybrids from contact zones compared to reference allopatric populations. The distribution of mtDNA haplotypes followed geographic rather than species boundaries. Additionally, no new haplotypes emerged in the contact zones, instead these zones contained the most common local variants. However, species diverged significantly at nuclear genomes and populations in contact zones exhibited similar or higher genetic diversity compared to the reference stands. There were no signs of admixture in any allopatric population, while clear admixture was evident in the contact zones, indicating that hybridization has a geographically localized effect on the genetic variation of the analyzed pine species.

Conclusions
Our results suggest that hybrid zones act as sinks rather than melting pots of genetic diversity. Hybridization influences sympatric populations but is confined to contact zones. The spectrum of parental species ancestry in hybrids reflects the old evolutionary history of the sympatric populations. These findings also imply that introgression may play a crucial role in the adaptation of hybrids to specific environments.
Impact of semaglutide on weight and functional outcomes among obese heart failure patients: a propensity scores matching analysis
Authors: Balata Mahmoud , Becher Marc Ulrich 
Published: October 2024

Abstract
Background & objectives
Obesity is a common comorbidity in heart failure, yet effective pharmacological options for weight loss in these patients are limited. Semaglutide, a glucagon-like peptide 1 receptor agonist, has shown promise for weight reduction in obese adults. This study aims to evaluate semaglutide’s impact on weight loss, functional status, and clinical outcomes in obese patients with heart failure.

Methods
A retrospective analysis was conducted on all consecutive obese (BMI > 30 kg/m²) patients with heart failure at the University Hospital Bonn outpatient clinic from July 2019 to July 2022. Propensity score matching paired patients receiving semaglutide as an add-on therapy (SEMA) with those on medical therapy alone (Control).

Results
Among 1,942 patients with heart failure screened, 26 matched pairs were identified. At one year, the SEMA group exhibited significant weight loss, with a mean BMI reduction of -2.91 kg/m² (95% CI: -4.27 to -1.55; p < 0.001), while the control group showed a non-significant mean change of -0.41 kg/m² (95% CI: -1.08 to 0.26; p = 0.22). The difference in BMI between the two groups was statistically significant (mean difference: 3.42 kg/m², 95% CI: 1.43 to 5.42; p = 0.001). Improvements by at least one NYHA class were observed in 65% of the SEMA group (p < 0.001) compared to 15% of the control group (p = 0.18). The SEMA group also showed a significant increase in 6-minute walk distance (6MWD), with a mean difference of 75 m between the groups at one year (95% CI: 0.53 to 150.02; p = 0.049). NT-proBNP levels significantly decreased in the SEMA group (p < 0.001) compared to the control group (p = 0.78), with a statistically significant difference in NT-proBNP between the groups (p = 0.048). Both improvements in 6MWD and reductions in NT-proBNP were significantly correlated with BMI percentage reductions.

Conclusions
Semaglutide was associated with significant weight reduction in obese patients with heart failure, accompanied by improved NYHA classification and 6-minute walk distance. Larger, multi-center trials and prospective, randomized controlled trials are warranted. These studies should focus on assessing long-term outcomes, optimizing dosage, and exploring the potential cardiovascular benefits beyond weight reduction.
Estimating reference intervals from an IPD meta-analysis using quantile regression
Authors: Jiang Ziren , Chu Haitao , Wang Zhen , Murad M. Hassan , Siegel Lianne K. 
Published: October 2024

Abstract
Background
Reference intervals, which define an interval in which a specific proportion of measurements from a healthy population are expected to fall, are commonly used in medical practice. Synthesizing information from multiple studies through meta-analysis can provide a more precise and representative reference interval than one derived from a single study. However, the current approaches for estimating the reference interval from a meta-analysis mainly rely on aggregate data and require parametric distributional assumptions that cannot always be checked.

Methods
With the availability of individual participant data (IPD), non-parametric methods can be used to estimate reference intervals without any distributional assumptions. Furthermore, patient-level covariates can be introduced to estimate personalized reference intervals that may be more applicable to specific patients. This paper introduces quantile regression as a method to estimate the reference interval from an IPD meta-analysis under the fixed effects model.

Results
We compared several non-parametric bootstrap methods through simulation studies to account for within-study correlation. Under fixed effects model, we recommend keeping the studies fixed and only randomly sampling subjects with replacement within each study.

Conclusion
We proposed to use the quantile regression in the IPD meta-analysis to estimate the reference interval. Based on the simulation results, we identify an optimal bootstrap strategy for estimating the uncertainty of the estimated reference interval. An example of liver stiffness measurements, a clinically important diagnostic test without explicitly established reference range in children, is provided to demonstrate the use of quantile regression in estimating both overall and subject-specific reference intervals.