bluuebunny/crossref_metadata_2025_split huggingface dataset:
De opmars van het uitleveringsrecht in de rechtspraak van het Hof van Justitie – het arrest Pisciotti nader beschouwd in het licht van eerdere Europese jurisprudentie en de Nederlandse uitleveringspraktijk
Authors: de Vries A.J. 
Published: February 2019

De opmars van het uitleveringsrecht in de rechtspraak van het Hof van Justitie – het arrest Pisciotti nader beschouwd in het licht van eerdere Europese jurisprudentie en de Nederlandse uitleveringspraktijk
Recentelijk heeft het Hof van Justitie in de zaak Pisciotti opnieuw een oordeel gegeven over uitlevering van een EU-onderdaan naar een derde staat.HvJ (Grote Kamer) 10 april 2018, zaak C-191/16, Romano Pisciotti/Bundesrepublik Deutschland, ECLI:EU:C:2018:222. Het arrest bouwt voort op eerdere revolutionaire rechtspraak en verduidelijkt de ingezette lijn van het Hof van Justitie. Om die reden is de uitspraak bijzonder interessant. In deze bijdrage bespreek ik het oordeel van het Hof van Justitie in Pisciotti. Vervolgens plaats ik het arrest in de context van eerdere Europese jurisprudentie over uitlevering. Ik bekijk ook welke implicaties de rechtspraak van het Hof van Justitie heeft voor de rechtspraktijk hier te lande.HvJ (Grote Kamer) 10 april 2018, zaak C-191/16, Romano Pisciotti/Bundesrepublik Deutschland, ECLI:EU:C:2018:222.
Integración de las TIC en los procesos de enseñanza-aprendizaje de la especialidad de Pedagogía en los conservatorios superiores de música
Authors: Díez Latorre Nuria , Carrera Farran Xavier 
Published: February 2019

Esta investigación analiza la integración de Tecnologías de la Información y la Comunicación (TIC) en los procesos de enseñanza-aprendizaje de los futuros pedagogos de música. Para ello se ha tomado en consideración la perspectiva del profesorado de los departamentos de Pedagogía de 14 conservatorios superiores españoles. Los datos se han recogido a partir de un cuestionario y un grupo de discusión. En general la predisposición a utilizar las TIC es alta, pero se basa en criterios de acceso a recursos y eficacia en las tareas docentes más que al fomento de prácticas pedagógicas innovadoras.
This research analyzes how Information and Communication Technologies (ICT) are integrated into teaching and the learning process of future music pedagogues. For this purpose, integration of ICT and teaching perspective of 14 Spanish superior music conservatories was analyzed through the conduction of a survey and the evaluation of a discussion group. The results suggest that, in general, there is a high predisposition towards the use of ICTs. They also show that this predisposition is based on access to resources and the efficiency in teaching tasks criteria rather than the promotion of innovative pedagogical practices.
СТРУКТУРНЫЕ ТРАНСФОРМАЦИИ УГЛЕРОДНОГО НАНОМАТЕРИАЛА ПРИ ВОЗДЕЙСТВИИ ВЫСОКОЭНЕРГЕТИЧЕСКОГО ЛАЗЕРНОГО ИЗЛУЧЕНИЯ
Authors: Pavlyuchenko P.E. , Seropyan G.M. , Trenikhin M.V. , Drozdov V.A. 
Published: February 2019

В работе проведено облучение глобулярного турбостратного углеродного материала лазерными импульсами в различных условиях. Методом ПЭМ зафиксировано, что формирование наноструктурированных частиц – нанокапсул в процессе облучения на длине волны 1064 нм происходит при плотности мощности I =108 Вт/см2, а её увеличение до I =1011 Вт/см2 приводит к возрастанию упорядоченности получаемых структур. Показано, что облучение исходных глобул импульсами с длиной волны 532 нм позволяет уменьшить межплоскостное расстояние графеновых слоев с 0,410 нм до 0,346 нм в сформированных нанокапсулах. Проанализирована зависимость максимальной температуры нагрева наночастиц от плотности энергии в пятне лазера. Установлено влияние температуры нагрева углеродного наноматериала на его структуру и морфологию.
Wild edible fool’s watercress, a potential crop with high nutraceutical properties
Authors: Guijarro-Real Carla , Prohens Jaime , Rodriguez-Burruezo Adrian , Adalid-Martínez Ana María , López-Gresa M Pilar , Fita Ana 
Published: February 2019

BackgroundFool’s watercress (Apium nodiflorum) is an edible vegetable with potential as a new crop. However, little information is available regarding the antioxidant properties of the plant and the individual phenolics accounting for this capacity are unknown.MethodsThe antioxidant properties of twenty-five wild populations were analysed and individual phenolics present in the species reported and compared with celery and parsley. The antioxidant activity was measured as the 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) free radical scavenging capacity, and the total phenolics content (TPC) via the Folin-Ciocalteu procedure. The individual phenolics constituents were determined via high performance liquid chromatography (HPLC) as aglycones.ResultsThe average DPPH and TPC of fool’s watercress were 28.1 mg Trolox g−1DW and 22.3 mg of chlorogenic acid equivalents g−1DW, respectively, much higher than those of celery and parsley. Significant differences for both DPPH and TPC, which may be explained by either genotype or environmental factors, were detected among groups established according to geographical origin. Quercetin was identified as the major phenolic present in the leaves of the species, unlike parsley and celery, in which high amounts of apigenin and luteolin were determined. Quercetin represented 61.6% of the phenolics targeted in fool’s watercress, followed by caffeic acid derivatives as main hydroxycinnamic acids.DiscussionThe study reports the high antioxidant properties of fool’s watercress based on a large number of populations. Results suggest that quercetin accounts for an important share of the antioxidant capacity of this potential new crop. The study also provides a basis for future breeding programs, suggesting that selection by geographical locations may result in differences in the antioxidant properties.
A gelatin/collagen/polycaprolactone scaffold for skin regeneration
Authors: Wei Lin-Gwei , Chang Hsin-I , Wang Yiwei , Hsu Shan-hui , Dai Lien-Guo , Fu Keng-Yen , Dai Niann-Tzyy 
Published: February 2019

BackgroundA tissue-engineered skin substitute, based on gelatin (“G”), collagen (“C”), and poly(ε-caprolactone) (PCL; “P”), was developed.MethodG/C/P biocomposites were fabricated by impregnation of lyophilized gelatin/collagen (GC) mats with PCL solutions, followed by solvent evaporation. Two different GC:PCL ratios (1:8 and 1:20) were used.ResultsDifferential scanning calorimetry revealed that all G/C/P biocomposites had characteristic melting point of PCL at around 60 °C. Scanning electron microscopy showed that all biocomposites had similar fibrous structures. Good cytocompatibility was present in all G/C/P biocomposites when incubated with primary human epidermal keratinocytes (PHEK), human dermal fibroblasts (PHDF) and human adipose-derived stem cells (ASCs)in vitro. All G/C/P biocomposites exhibited similar cell growth and mechanical characteristics in comparison with C/P biocomposites. G/C/P biocomposites with a lower collagen content showed better cell proliferation than those with a higher collagen contentin vitro. Due to reasonable mechanical strength and biocompatibilityin vitro, G/C/P with a lower content of collagen and a higher content of PCL (GCLPH) was selected for animal wound healing studies. According to our data, a significant promotion in wound healing and skin regeneration could be observed in GCLPHseeded with adipose-derived stem cells by Gomori’s trichrome staining.ConclusionThis study may provide an effective and low-cost wound dressings to assist skin regeneration for clinical use.
Food for thought: Exploring associations between knowledge of the Healthy Living Pyramid, the Australian Guide to Healthy Eating and food group intake among Western Australian adolescents
Authors: Farringdon Fiona , Farringdon Samuel , Chivers Paola 
Published: February 2019

AbstractIssue addressedEvidence indicates adolescents do not comply with the Australian Guide to Healthy Eating (AGHE)‐recommended daily serves (RDS). Little research has identified their level of knowledge regarding the guidelines and associations with food intake.MethodsSurvey methodology was used to measure knowledge of the Healthy Living Pyramid (HLP) and the AGHE‐RDS. Food group intake was measured using a modified food frequency questionnaire. A generalised linear model (GLM) investigated the associations between the HLP, RDS knowledge and daily equivalent frequencies (DEFs) for each food group.ResultsThe study comprised 190 students aged 14‐15 years. The majority reported accurate knowledge of the HLP. Only fruit and vegetable RDS was correctly identified. Participants did not meet DEFs for any food group. For both DEF grains (P = 0.004) and vegetables (P = 0.023) participants with correct knowledge reported a DEF closer to the RDS. For DEF‐baked goods and snacks (P = 0.012), participants reporting correct knowledge also reported DEF closer to the RDS. No associations were identified for the other food groups.So what?Knowledge of the HLP was more accurate than the AGHE‐RDS. Knowledge of fruit and vegetable RDS was better than the other food groups. The positive associations between knowledge and compliance with the RDS suggest knowledge can influence food choice. However, the lack of associations between knowledge and consumption for most food groups suggests clear “do” messages, similar to Go for 2&5, about the RDS for all food groups should be developed for adolescents.
Environmental Niche Adaptation Revealed Through Fine Scale Phenological Niche Modeling
Authors: Dam Matthew H. Van , Rominger Andrew J. , Brewer Michael S. 
Published: February 2019

ABSTRACTAimPhenology, the temporal response of a population to its climate, is a crucial behavioral trait shared across life on earth. How species adapt their phenologies to climate change is poorly understood but critical in understanding how species will respond to future change. We use a group of flies (Rhaphiomidas) endemic to the North American deserts to understand how species adapt to changing climatic conditions. Here we explore a novel approach for taxa with constrained phenologies aimed to accurately model their environmental niche and relate this to phenological and morphological adaptations in a phylogenetic context.TaxonInsecta, Diptera, Mydidae,RhaphiomidasLocationNorth America, Mojave, Sonoran and Chihuahuan Deserts.MethodsWe gathered geographical and phenological occurrence data for the entire genusRhaphiomidas, and, estimated a time calibrated phylogeny. We compared Daymet derived temperature values for a species adult occurrence period (phenology) with those derived from WorldClim data that is partitioned by month or quarter to examine what effect using more precise data has on capturing a species’ environmental niche. We then examined to what extent phylogenetic signal in phenological traits, climate tolerance and morphology can inform us about how species adapt to different environmental regimes.ResultsWe found that the Bioclim temperature data, which are averages across monthly intervals, poorly represent the climate windows to which adult flies are actually adapted. Using temporally-relevant climate data, we show that many species use a combination of morphological and phenological changes to adapt to different climate regimes. There are also instances where species changed only phenology to track a climate type or only morphology to adapt to different environments.Main ConclusionsWithout using a fine-scale phenological data approach, identifying environmental adaptations could be misleading because the data do not represent the conditions the animals are actually experiencing. We find that fine-scale phenological niche models are needed when assessing taxa that have a discrete phenological window that is key to their survival, accurately linking environment to morphology and phenology. Using this approach, we show thatRhaphiomidasuse a combination of niche tracking and adaptation to persist in new niches. Modeling the effect of phenology on such species’ niches will be critical for better predictions of how these species might respond to future climate change.
Sex differences in the effects of gonadal hormones on white matter microstructure development in adolescence
Authors: Ho Tiffany C. , Oskirko Kira , Colich Natalie L. , Sisk Lucinda M. , Gotlib Ian H. 
Published: February 2019

AbstractAdolescence, the transition between childhood and adulthood, is characterized by rapid brain development in white matter (WM) that is attributed in part to surges in gonadal hormones. To date, however, there have been no longitudinal investigations of the effects of gonadal hormones on WM development in adolescents. We acquired T1-weighted and diffusion-weighted MRIs at two timepoints and saliva samples from 80 adolescents (52 females [11.10±1.05 years at Time 1; 12.75±1.37 years at Time 2] and 28 males [ages 11.91±0.88 years at Time 1; 13.79±0.95 years at Time 2] who were matched on pubertal stage at Time 1. We estimated mean fractional anisotropy (FA) from 10 major WM tracts and assayed levels of testosterone (in both sexes) and estradiol (in females only). We used linear regression models to test whether interactions between sex and changes in testosterone levels significantly explained changes in FA. Sex significantly moderated the associations between changes in testosterone and changes in FA within the corpus callosum, inferior fronto-occipital fasciculus (IFOF), uncinate fasciculus (UF), and cingulum cingulate (all ps<0.05): whereas these associations were positive in females, they were not significant in males. Females also exhibited positive associations between changes in estradiol and changes in UF, IFOF, and corticospinal FA (all ps<0.05). Our findings indicate that sex differences in WM microstructure of tracts supporting cognitive control, response inhibition, and emotion regulation are explained by differences in changes in testosterone, and have important implications for understanding sex differences in brain development and psychosocial behaviors during the pubertal transition.
Microbial metabolism and adaptations in Atribacteria-dominated methane hydrate sediments
Authors: Glass Jennifer B. , Ranjan Piyush , Kretz Cecilia B. , Nunn Brook L. , Johnson Abigail M. , Xu Manlin , McManus James , Stewart Frank J. 
Published: February 2019

SummaryGas hydrates harbor gigatons of natural gas, yet their microbiomes remain understudied. We bioprospected 16S rRNA amplicons, metagenomes, and metaproteomes from methane hydrate-bearing sediments under Hydrate Ridge (offshore Oregon, USA, ODP Site 1244, 2-69 mbsf) for novel microbial metabolic and biosynthetic potential. Atribacteria sequences generally increased in relative sequence abundance with increasing sediment depth. Most Atribacteria ASVs belonged to JS-1-Genus 1 and clustered with other sequences from gas hydrate-bearing sediments. We recovered 21 metagenome-assembled genomic bins spanning three geochemical zones in the sediment core: the sulfate-methane transition zone, metal (iron/manganese) reduction zone, and gas hydrate stability zone. We found evidence for bacterial fermentation as a source of acetate for aceticlastic methanogenesis and as a driver of iron reduction in the metal reduction zone. In multiple zones, we identified a Ni-Fe hydrogenase-Na+/H+ antiporter supercomplex (Hun) in Atribacteria and Firmicutes bins and in other deep subsurface bacteria and cultured hyperthermophiles from the Thermotogae phylum. Atribacteria expressed tripartite ATP-independent (TRAP) transporters downstream from a novel regulator (AtiR). Atribacteria also possessed adaptations to survive extreme conditions (e.g., high salt brines, high pressure, and cold temperatures) including the ability to synthesize the osmolyte di-myo-inositol-phosphate as well as expression of K+-stimulated pyrophosphatase and capsule proteins.Originality-Significance StatementThis work provides insights into the metabolism and adaptations of microbes that are ubiquitous and abundant in methane-rich ecosystems. Our findings suggest that bacterial fermentation is a source of acetate for aceticlastic methanogenesis and a driver of iron reduction in the metal reduction zone. Atribacteria, the most abundant phylum in gas hydrate-bearing sediments, possess multiple strategies to cope with environmental stress.
Comparative genome analysis indicates rapid evolution of pathogenicity genes inColletotrichum tanaceti
Authors: Lelwala Ruvini V. , Korhonen Pasi K. , Young Neil D. , Scott Jason B. , Ades Peter K. , Gasser Robin B. , Taylor Paul W. J. 
Published: February 2019

ABSTRACTColletotrichum tanacetiis an emerging foliar fungal pathogen of pyrethrum (Tanacetum cinerariifolium), posing a threat to the global pyrethrum industry. Despite being reported consistently from field surveys in Australia, the molecular basis of pathogenicity ofC. tanacetion pyrethrum is unknown. Herein, the genome ofC. tanaceti(isolate BRIP57314) was assembledde novoand annotated using transcriptomic evidence. The inferred pathogenicity gene suite ofC. tanaceticomprised a large array of genes encoding secreted effectors, proteases, CAZymes and secondary metabolites. Comparative analysis of its CAZyme pathogenicity profiles with those of closely related species suggested thatC. tanacetihad additional hosts to pyrethrum. The genome ofC. tanacetihad a high repeat content and repetitive elements were located significantly closer to genes inferred to influence pathogenicity than other genes. These repeats are likely to have accelerated mutational and transposition rates in the genome, resulting in a rapid evolution of certain CAZyme families in this species. TheC. tanacetigenome consisted of a gene-sparse, A-T rich region facilitating a “two-speed” genome. Pathogenicity genes within this region were likely to have a higher evolutionary rate than the ‘core’ genome. This “two-speed” genome phenomenon in certainColletotrichumspp. was hypothesized to have caused the clustering of species based on the pathogenicity genes, to deviate from taxonomy. With the large repertoire of pathogenicity factors that can potentially evolve rapidly in response to control measures,C. tanacetimay pose a high-risk to global pyrethrum production. Knowledge of the pathogenicity genes will facilitate future research in disease management ofC. tanacetiand otherColletotrichumspp..
TheArabidopsis thalianapan-NLRome
Authors: Van de Weyer Anna-Lena , Monteiro Freddy , Furzer Oliver J. , Nishimura Marc T. , Cevik Volkan , Witek Kamil , Jones Jonathan D.G. , Dangl Jeffery L. , Weigel Detlef , Bemm Felix 
Published: February 2019

AbstractDisease is both among the most important selection pressures in nature and among the main causes of yield loss in agriculture. In plants, resistance to disease is often conferred by Nucleotide-binding Leucine-rich Repeat (NLR) proteins. These proteins function as intracellular immune receptors that recognize pathogen proteins and their effects on the plant. Consistent with evolutionarily dynamic interactions between plants and pathogens, NLRs are known to be encoded by one of the most variable gene families in plants, but the true extent of intraspecific NLR diversity has been unclear. Here, we define the majority of theArabidopsis thalianaspecies-wide “NLRome”. From NLR sequence enrichment and long-read sequencing of 65 diverseA. thalianaaccessions, we infer that the pan-NLRome saturates with approximately 40 accessions. Despite the high diversity of NLRs, half of the pan-NLRome is present in most accessions. We chart the architectural diversity of NLR proteins, identify novel architectures, and quantify the selective forces that act on specific NLRs, domains, and positions. Our study provides a blueprint for defining the pan-NLRome of plant species.
A genome-based species taxonomy of theLactobacillusGenus Complex
Authors: Wittouck Stijn , Wuyts Sander , Meehan Conor J , Noort Vera van , Lebeer Sarah 
Published: February 2019

AbstractBackgroundThere are over 200 published species within theLactobacillusGenus Complex (LGC), the majority of which have sequenced type strain genomes available. Although gold standard, genome-based species delimitation cutoffs are accepted by the community, they are seldom checked against currently available genome data. In addition, there are many species-level misclassification issues within the LGC. We constructed ade novospecies taxonomy for the LGC based on 2,459 publicly available, decent-quality genomes and using a 94% core nucleotide identity threshold. We reconciled thesede novospecies with published species and subspecies names by (i) identifying genomes of type strains in our dataset and (ii) performing comparisons based on 16S rRNA sequence identity against type strains.ResultsWe found that genomes within the LGC could be divided into 239 clusters (de novospecies) that were discontinuous and exclusive. Comparison of thesede novospecies to published species lead to the identification of ten sets of published species that can be merged and one species that can be split. Further, we found at least eight genome clusters that constitute new species. Finally, we were able to accurately classify 98 unclassified genomes and reclassify 74 wrongly classified genomes.ConclusionsThe current state of LGC species taxonomy is largely consistent with genome data, but there are some inconsistencies as well as genome misclassifications. These inconsistencies should be resolved to evolve towards a meaningful taxonomy where species have a consistent size in terms of sequence divergence.
Pyrethroid exposure altersAnopheles albimanusmicrobiota and resistant mosquitoes harbor more insecticide-metabolizing bacteria
Authors: Dada Nsa , Lol Juan C. , Benedict Ana Cristina , López Francisco , Sheth Mili , Dzuris Nicole , Padilla Norma , Lenhart Audrey 
Published: February 2019

AbstractA deeper understanding of the mechanisms underlying insecticide resistance is needed to mitigate its threat to malaria vector control. Building upon our earlier identified associations between mosquito microbiota and insecticide resistance, we demonstrate for the first time, type-specific effects of pyrethroid exposure on internal and cuticle surface bacteria in adult progeny of field-collectedAnopheles albimanus. In contrast, larval cuticle surface—but not internal—bacteria were affected by pyrethroid exposure. Being over five-folds more abundant in pyrethroid resistant adults, as compared to susceptible or non-insecticide-exposed mosquitoes,Klebsiella(alphacypermethrin),PantoeaandAsaia(permethrin) were identified as potential markers of pyrethroid resistance inAn. albimanus. We also show for the first time thatAn. albimanuslarvae and adult cuticles harbor more diverse bacterial communities than their internal microbial niches. Our findings indicate insecticide selection pressures on mosquito microbiota, and support the hypothesis of an undescribed microbe-mediated mechanism of insecticide metabolism in mosquitoes.
A Moving Target: The Megaplasmid pMPPla107 Sensitizes Cells to an Inhibitory Agent Conserved AcrossPseudomonas spp
Authors: Smith Brian A. , Feinstein Yelena , Clark Meara , Baltrus David A. 
Published: February 2019

ABSTRACTThe widespread use of antibiotics across clinical and agricultural settings results in strong selection pressures and contributes to the fixation of antibiotic resistance genes, the presence of which lowers the efficacy of proven treatments for infection. Furthermore, plasmids are often key vectors that facilitate the rapid dispersal of antibiotic resistance genes across bacterial strains via horizontal gene transfer. In contrast to previous widespread correlations between plasmid acquisition and resistance to antimicrobial compounds, we demonstrate that acquisition of theP. syringaemegaplasmid pMPPla107 sensitizes strains to a conserved, bacteriostatic small molecule produced by manyPseudomonas spp. Moreover, we find the acquisition of pMPPla107 reduces production of the inhibitory agent. Our results provide insights and suggest new directions to investigate collateral sensitivity to antimicrobials due to plasmid acquisition as well as highlight costs associated to horizontal gene transfer in the form of sensitivity to antagonistic microbial interactions.