bluuebunny/crossref_metadata_2025_split huggingface dataset:
An integrative transcriptome analysis reveals a functional role for thyroid transcription factor‐1 in small cell lung cancer
Authors: Horie Masafumi , Miyashita Naoya , Mattsson Johanna Sofia Margareta , Mikami Yu , Sandelin Martin , Brunnström Hans , Micke Patrick , Nagase Takahide , Saito Akira 
Published: June 2018

AbstractSmall cell lung cancer (SCLC) is a neuroendocrine tumour that exhibits rapid growth and metastatic spread. Although SCLC represents a prototypically undifferentiated cancer type, thyroid transcription factor‐1 (TTF‐1, gene symbol NKX2‐1), a master regulator for pulmonary epithelial cell differentiation and lung morphogenesis, is strongly upregulated in this aggressive cancer type. The aim of this study was to evaluate a functional role for TTF‐1 in SCLC. We demonstrated that achaete‐scute complex homolog 1 (ASCL1), an essential transcription factor for neuroendocrine differentiation, positively regulated TTF‐1 in SCLC cell lines. Subsequently, we described genes and microRNAs (miRNAs) that were possibly controlled by TTF‐1 and identified nuclear factor IB (NFIB), a recently characterised driver of SCLC progression, as a transcriptional target of TTF‐1. Our findings shine light on a regulatory axis in SCLC consisting of ASCL1/TTF‐1/NFIB that potentially contributes to the tumourigenesis of SCLC. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Outcome of children and adolescents with Down syndrome treated on Dana‐Farber Cancer Institute Acute Lymphoblastic Leukemia Consortium protocols 00–001 and 05‐001
Authors: Athale Uma H. , Puligandla Maneka , Stevenson Kristen E. , Asselin Barbara , Clavell Luis A. , Cole Peter D. , Kelly Kara M. , Laverdiere Caroline , Leclerc Jean‐Marie , Michon Bruno , Schorin Marshall A. , Sulis Maria Luisa , Welch Jennifer J. G. , Harris Marian H. , Neuberg Donna S. , Sallan Stephen E. , Silverman Lewis B. 
Published: June 2018

AbstractBackgroundChildren and adolescents with Down syndrome (DS) and acute lymphoblastic leukemia (ALL) are reported to have increased relapse rates and therapy‐related mortality (TRM). Treatment regimens for DS‐ALL patients often include therapy modifications. Dana‐Farber Cancer Institute (DFCI) ALL Consortium protocols have used same risk‐stratified treatment for patients with and without DS.ProceduresWe compared clinical and outcome data of DS (n = 38) and non‐DS (n = 1,248) patients enrolled on two consecutive DFCI ALL trials 00–001 (2000–2004) and 05‐001 (2005–2011) with similar risk adapted therapy regardless of DS status.ResultsThere was no difference in demographic or presenting clinical features between two groups except absence of T‐cell phenotype and lower frequency of hyperdiploidy in DS‐ALL group. All DS‐ALL patients achieved complete remission; four relapsed and one subsequently died. There was no TRM in DS‐ALL patients. DS‐ALL patients had significantly higher rates of mucositis (52% vs. 12%, p < 0.001), non‐CNS thrombosis (18% vs. 8%; p = 0.036), and seizure (16% vs. 5%, p = 0.010). Compared to non‐DS‐ALL patients, DS‐ALL patients had a higher incidence of infections during all therapy phases. The 5‐year event‐free and overall survival rates of DS‐ALL patients were similar to non‐DS‐ALL patients (91% [95% confidence interval (CI), 81–100] vs. 84% [95% CI, 82–86]; 97% [95% CI, 92–100] vs. 91% [95% CI, 90–93]).ConclusionThe low rates of relapse and TRM indicate that uniform risk‐stratified therapy for DS‐ALL and non‐DS‐ALL patients on DFCI ALL Consortium protocols was safe and effective, although the increased rate of toxicity in the DS‐ALL patients highlights the importance of supportive care during therapy.
Biology of human primitive erythroblasts for application in noninvasive prenatal diagnosis
Authors: Huang Zhou‐Wei , Fong Chui‐Yee , Gauthaman Kalamegam , Sukumar Ponnusamy , Mahyuddin Aniza P. , Barrett Angela N. , Bongso Ariff , Choolani Mahesh 
Published: June 2018

AbstractObjectiveHuman primitive erythroblasts produced during early embryogenesis have been found in maternal circulation at early gestation and are considered good target cells for noninvasive prenatal diagnosis. We aimed to gain a better understanding of the biology of primitive erythroblasts and maximize their potential utility for noninvasive prenatal diagnosis.MethodsCells were obtained from first trimester human placental tissues. Biological properties including surface antigen composition, differentiation, proliferation, enucleation, and degeneration were studied as gestation progressed. A microdroplet culture system was developed to observe the behavior of these cells in vitro.ResultsHistology showed that primitive erythroblasts undergo maturation from polychromatic to orthochromatic erythroblasts and can differentiate spontaneously in vitro. Cell surface markers and nuclear gene expression suggest that the cells do not possess stemness properties, despite being primitive in nature. They have limited proliferative activity and highly deacetylated chromatin, but a microdroplet culture system can prolong their viability under normoxic conditions. No apoptosis was seen by 11 weeks' gestation, and there was no enucleation in vitro.ConclusionThese properties confirm that viable cells with intact nuclei can be obtained at very early gestation for genetic analysis.
Photocatalytic degradation of organic dyes by a donor–acceptor type conjugated polymer: poly(thiophene‐1,3,4‐oxadiazole) and its photocatalytic mechanism
Authors: Yang Xiao , Duan Lian , Ran Xiaoqi 
Published: June 2018

AbstractA donor–acceptor conjugated‐type polymer, poly(thiophene‐1,3,4‐oxadiazole) (PThOD), was synthesized by one‐step polycondensation in polyphosphoric acid. PThOD was confirmed and characterized using Fourier transform infrared spectra, thermogravimetric analysis, X‐ray diffraction and UV‐visible spectra. The UV‐visible spectra showed that PThOD could absorb not only in the whole ultraviolet range from 200 to 400 nm but also in the visible light range up to 500 nm. Considering the conjugated structure of PThOD, studies were carried out of its performance for the photocatalytic degradation of various dyes, such as methyl orange, methylene blue, rhodamine B and reactive brilliant blue (RBB), under medium‐pressure Hg light source irradiation. The results showed that PThOD was an effective photocatalyst, which could degrade the four types of dye solution. Besides, visible light was also used to investigate the degradation of RBB, and the results showed that RBB could be degraded as well, though the efficiency was not as high as using the Hg lamp. The superoxide anion radical (O2•−), which is one of the reactive oxide species, was detected using the nitrotetrazolium blue chloride method and considered to play a key role in photodegradation of dyes. Moreover, various scavengers were also used to further prove the function of −. Furthermore, it was found that degradation rate could be changed by adding H+, which might be attributed to a change of adsorption capacity of PThOD. Finally, the reusability of PThOD as photocatalyst was investigated. The results indicated that the reusability efficiency of PThOD was excellent even after ten continuous rounds of use. © 2018 Society of Chemical Industry
Analytical optimization of intermediate band systems: Achieving the best of two worlds
Authors: Suchet Daniel , Delamarre Amaury , Cavassilas Nicolas , Jehl Zacharie , Okada Yoshitaka , Sugiyama Masakazu , Guillemoles Jean‐Francois 
Published: June 2018

AbstractLagrange multipliers provide a powerful framework to devise the optimization of systems under constraints. It can be especially useful in the context of photovoltaics, where electrical or structural continuity relations impose connections between quantities, such as current matching between the different subcells of tandem devices. In this work, we apply this method to Intermediate band solar cell, an advanced concept for solar energy conversion in which 2 low‐energy photons can promote an electron to the conduction band through a so‐called intermediate band. We demonstrate that an intermediate band solar cell under solar spectrum cannot meet voltage preservation and current matching at the same time. By contrast, we show that the implementation of an energy shift (electronic ratchet) in any of the bands lifts one of the constraints set on the system and allows those 2 criteria to be filled simultaneously. Additional insights are provided by the numerical study of the short‐circuit current and fill factor of the systems at stake, which show that a system with ratchet benefits from the same current increase as a standard intermediate band solar cell (same short‐circuit current), while maintaining I‐V properties of a single junction (same open‐voltage circuit, same fill factor).
High‐Strength Sub‐Micrometer Spherical Particles Fabricated by Pulsed Laser Melting in Liquid
Authors: Kondo Mitsuhiko , Shishido Nobuyuki , Kamiya Shoji , Kubo Atsushi , Umeno Yoshitaka , Ishikawa Yoshie , Koshizaki Naoto 
Published: June 2018

AbstractSub‐micrometer spherical particles that are obtained by pulsed laser melting in liquid (PLML) are usually observed to be single crystalline, and it is suggested that they are mechanically very strong. In this study, fracture tests of various sub‐micrometer spherical particles are performed by compressive force application. The results indicate that B4C and TiO2 sub‐micrometer spherical particles exhibit brittle fracture behavior under tensile fracture mode at the center of the particles. The fracture strength of the sub‐micrometer spherical particles is larger than that of the bulk material reported in the literature by about one order of magnitude. TiO2 sub‐micrometer spherical particles obtained by PLML are stronger than the commercially available TiOx sub‐micrometer spherical particles with a porous structure. In addition, due to the single crystallinity of particles, smaller particles have larger fracture strength, becoming up to 10–40% of ideal tensile fracture strength calculated based on density functional theory. Thus, these results demonstrate that sub‐micrometer spherical particles obtained using PLML exhibit fairly strong and unique mechanical properties, and therefore they are very promising for various mechanical applications at the sub‐micrometer size scale.
Hypersonic‐Induced 3D Hydrodynamic Tweezers for Versatile Manipulations of Micro/Nanoscale Objects
Authors: Cui Weiwei , He Meihang , Yang Yang , Zhang Hongxiang , Pang Wei , Duan Xuexin 
Published: June 2018

AbstractDeveloping microrobots for precisely manipulating micro/nanoscale objects has triggered tremendous research interest for various applications in biology, chemistry, physics, and engineering. Here, a novel hypersonic‐induced hydrodynamic tweezers (HSHTs), which use gigahertz nano‐electromechanical resonator to create localized 3D vortex streaming array for the capture and manipulation of micro‐ and nanoparticles in three orientations: transportation in a plane and self‐rotation in place, are presented. 3D vortex streaming can effectively pick up particles from the flow, whereas the high‐speed rotating vortices are used to drive self‐rotation simultaneously. By tuning flow rate, the captured particles can be delivered, queued, and selectively sorted through the 3D HSHTs. Through numerical simulations and theoretical analysis, the generation of the 3D vortex and the mechanism of the particles manipulation by ultrahigh frequency acoustic wave are demonstrated. Benefitting from the advantages of the acoustic and hydrodynamic method, the developed HSHTs work in a precise, noninvasive, label‐free, and contact‐free manner, enabling wide applications in micro/nanoscale manipulations and biomedical research.
Unusually Large Lattice Mismatch‐Induced Optical Behaviors of Au@Cu–Cu2O Core–Shell Nanocrystals with Noncentrally Located Cores
Authors: Hsia Chi‐Fu , Chang Chia‐Hao , Huang Michael H. 
Published: June 2018

AbstractTo extend the optical property characterization of metal–Cu2O polyhedra, 50 nm Au@Cu cubic cores are used to fabricate Au@Cu–Cu2O core–shell cubes, octahedra, and rhombic dodecahedra with tunable sizes. Despite the unusually large lattice mismatch of 15.1% between Cu and Cu2O, fine adjustment in the volumes of reagents introduced allows the formation of these heterostructures. To relieve the lattice strain, the metal cores are essentially never found to locate at the particle center, and slight lattice spacing shifts are recorded. Although efforts are made to reduce the heterostructure sizes, the Cu2O shells are generally too thick to reveal surface plasmon resonance (SPR) absorption band from the metal cores. Only the Au@Cu–Cu2O cubes with many cores located near the particle corners show observable SPR band red‐shift, but UV–vis spectra of all particle shapes are still dominated by Cu2O absorption and light scattering bands. Au@Cu–Cu2O cubes consistently show the most red‐shifted absorption bands than those of octahedra resulting from the optical facet effects.
The binding properties of cycloxaprid on insect native nAChRs partially explain the low cross‐resistance with imidacloprid in Nilaparvata lugens
Authors: Zhang Yixi , Xu Xiaoyong , Bao Haibo , Shao Xusheng , Li Zhong , Liu Zewen 
Published: June 2018

AbstractBACKGROUNDNeonicotinoids, such as imidacloprid, are selective agonists of insect nicotinic acetylcholine receptors (nAChRs) used to control Nilaparvata lugens, a major rice insect pest. High imidacloprid resistance has been reported in N. lugens both in the laboratory and in the field. Cycloxaprid (CYC), an oxa‐bridged cis‐nitromethylene neonicotinoid, showed high insecticidal activity against N. lugens and low cross‐resistance in imidacloprid‐resistant strains and field populations.RESULTSBinding studies demonstrated that imidacloprid has two binding sites with different affinities (Kd = 3.18 ± 0.43 pm and 1.78 ± 0.19 nm) in N. lugens nAChRs. CYC was poor at displacing [3H]imidacloprid at its high‐affinity binding site (Ki = 159.38 ± 20.43 nm), but quite efficient at the low‐affinity binding site (Ki = 1.27 ± 0.35 nm). These data showed that CYC had overlapping binding sites with imidacloprid only at its low‐affinity binding site. Therefore, the low displacement ability of CYC against imidacloprid binding at its high‐affinity site could partially explain the low cross‐resistance of CYC in imidacloprid‐resistant populations.CONCLUSIONThe high insecticidal activity, low cross‐resistance and different binding properties on insect nAChRs of CYC show that it is a potential insecticide for the control of N. lugens and related insect pests, especially ones with high resistance to neonicotinoids. © 2018 Society of Chemical Industry
The association between green tea consumption and breast cancer risk: A systematic review and meta‐analysis
Authors: Najaf Najafi Mona , Salehi Maryam , Ghazanfarpour Masumeh , Hoseini Zeinab Sadat , Khadem‐Rezaiyan Majid 
Published: June 2018

This systematic review and meta‐analysis aimed to critically evaluate the relation between green tea (GT) consumption and the risk of breast cancer. Popular electronic databases were systematically searched for papers in English language. All case‐control and cohort studies in addition to randomized clinical trials were included if they assessed the chemopreventive effects of GT on breast cancer. The quality of included studies was assessed using the Newcastle–Ottawa and Jadad scale. This systematic review comprised 14 studies: 9 case‐control studies, 4 cohort studies, and 1 clinical trial. Odds ratio (OR) in case‐control studies suggested that women in the group receiving the highest level of GT had 19% reduction in breast cancer risk compared with those who received the lowest level of GT (summary OR = 0.81, p = .031; 95% CI [0.66, 0.981]; heterogeneity, I2 = 71.53, p < .001, random effect model; 9 studies). OR in cohort studies also showed no significant difference (OR = 0.99, p = .94; 95% CI [0.81, 1.138]; heterogeneity, I2 = 19.06, p = .29; fixed‐effect model; 4 studies). According to the only clinical trial, treatment with GT could not alter the mammographic density compared with placebo (26% vs. 25%). It cannot be concluded that GT consumption may decrease the risk of breast cancer. Due to high heterogeneity, a pooled analysis of case‐control and cohort studies was not performed.
Fault trees analysis using expert opinion based on fuzzy‐bathtub failure rates
Authors: Nadjafi Mohammad , Farsi MohammadAli , Zio Enrico , Mousavi Arash K. 
Published: June 2018

AbstractThe main objective of fault tree analysis method is to estimate the “Top Event occurrence probability”. This requires determination of failure time distribution functions also known as “Bathtub Curves” for each of the system elements/events. This paper introduces a novel method to determine the failure time distribution functions using possibility theory. For this purpose, fuzzy‐bathtub distributions using expert opinions are generated for basic events and fuzzy formulas are derived for static and dynamic gates fault tree constructions. This process completed by proposed fuzzy Monte Carlo simulation throughout the preferred operational time and uses the actual time‐to‐failure data. Accordingly, the Top Event failure curve and the reliability profile of the system are depicted based on the defuzzificated basic‐events' bathtub‐failure‐rates. The results show that the proposed method not only is feasible and powerful but can also be accurate more than the other probabilistic and possibilistic techniques because of the component failure rates follow the real failure distributions.
Isolation and identification of potential fungal species for spent engine lubrication oil remediation in Peninsular Malaysia
Authors: Hock Ong Ghim , Cheng Ho Cheng , Fang Vivian Lim Bi , Yong Wong Yieng , Shing Wong Ling 
Published: June 2018

AbstractImproper disposal and spills of spent engine oil into the environment can result in contamination, which eventually affects humans through the food chain. Mycoremediation is an effective and inexpensive alternative to clean up spent engine oil contamination. In recent work, the potential effectiveness of fungi for degrading spent engine oil was confirmed, with the species identified through molecular identification. Fungi that were able to grow in Bushnell Haas Broth supplied with spent engine oil were identified with the potential to utilize spent engine oil as a carbon source. Six species of fungi namely Penicillium simplicissimum, Aspergillus nidulans, Aspergillus niger, Trichoderma longibrachiatum, Aspergillus ustus, and Aspergillus flavus were successfully identified in this study. Over a course of seven days, P. simplicissimum (21.11 percent) was identified as the most effective fungi in degrading spent engine oil, followed by A. nidulans (17.75 percent), A. niger (15.85 percent), T. longibrachiatum (15.12 percent), A. ustus (15.02 percent), and A. flavus (11.80 percent). As these species of fungi were isolated from the natural environment in Peninsular Malaysia, the potential of using these fungi as mycoremediation of spent engine oil was therefore confirmed.
Structural Modeling of New Polymorphism Clusters of HCV Polymerase Isolated from Direct‐Acting Antiviral Naïve Patients: Focus on Dasabuvir and Setrobuvir Binding Affinity
Authors: Romeo Isabella , Marascio Nadia , Pavia Grazia , Talarico Carmine , Costa Giosuè , Alcaro Stefano , Artese Anna , Torti Carlo , Liberto Maria Carla , Focà Alfredo 
Published: June 2018

AbstractManagement of Hepatitis C virus infection changed after the development of direct‐acting antiviral agents, including NS5B polymerase inhibitors. However, the presence of non‐synonymous substitutions could lead to therapy failure. Herein, we analyzed novel polymorphism clusters in NS5B polymerase from Hepatitis C virus (HCV) subtype 1b isolates of direct‐acting antivirals (DAAs) naïve patients by Sanger population sequencing. By means of computational approaches, we investigated the impact of these polymorphisms on the apo‐polymerase stability and on the binding affinity of dasabuvir and setrobuvir. Our thermodynamic and structural analysis highlighted that some mutational patterns could enhance or reduce the drug's affinity if compared to the wild‐type complexes and allowed us to well characterize the binding mode of the known drugs into the NS5B binding pocket. Our computational findings agreed with dasabuvir and setrobuvir in vitro reduced binding affinities against C316N mutant and could be useful in guiding the therapeutic approaches in presence of specific natural polymorphisms on HCV polymerase.
Synthesis, Antioxidant Evaluation, Density Functional Theory Study of Dihydropyrimidine Festooned Phenothiazines
Authors: Padmavathy Krishnaraj , Krishnan Kannan Gokula , Kumar Chandran Udhaya , Sutha Peramasivan , Sivaramakarthikeyan Ramar , Ramalingan Chennan 
Published: June 2018

AbstractA series of dihydropyrimidine decorated phenothiazine and their derivatives 7 a‐7 i possessing a diverse range of functionalities such as isopropyl, methoxy, chloro, fluoro, nitro, naphthyl have been synthesized by adopting a multistep synthetic strategy and characterized using various physical and spectroscopic techniques. Preliminary antioxidant evaluation of all the molecules has been executed and exerted activity upto 69.34% (IC50 148 μM) with respect to the standard ascorbic acid. Computational studies of a representative molecule 7 a have also been executed and appropriate results are compared with the experimental ones. The optimized structure with their bonding features and vibrational frequencies of 7 a have been determined by employing DFT−B3LYP method with 6–311++G(d,p) basis set. The theoretically calculated and experimentally resulted vibrational frequencies of 7 a are in good harmony with each other. Further, the 1H and 13C chemical shifts of 7 a have been calculated by the Gauge Independent Atomic Orbital (GIAO) technique and compared with the experimental ones.
Total Synthesis of Natural Product Piperodione and Its Analogues
Authors: Tiwari Praveen Kumar , Ballav Hemkalyan , Aidhen Indrapal Singh 
Published: June 2018

AbstractTwo new approaches for the synthesis of natural product piperodione, secondary metabolite have been achieved. Hitherto, unknown and easily accessible piperidine and morpholine based α‐ketoamide phosphoranes obtained from pyruvic acid have served as key building blocks for synthesis based on Wittig reaction. The alkylation of β‐keto esters with 3‐bromo derivative of pyruvic acid amides has been equally successful. Both the developed routes have enabled access to several new analogues of piperodione, opening up opportunities for systematic structure activity relationships studies during evaluation of biological activity.
Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract
Authors: Çimen Duygu , Bereli Nilay , Andaç Müge , Denizli Adil 
Published: June 2018

AbstractConcanavalin A, one of most studied lectins for the purification of glycoproteins and sugars, is selected as a model protein. In this study, a Concanavalin A imprinted poly(hydroxyethyl methacrylate) supermacroporous cryogel was prepared for the purification of Concanavalin from jack bean extract. N‐Methacryloyl‐l‐histidine methyl ester with nickel(II) ions was used as the metal ion coordination complex. Concanavalin A imprinted cryogels were characterized by swelling degree, surface area, Fourier transform infrared spectroscopy, scanning electron microscopy and micro computed tomography measurements. Concanavalin A rebinding and desorption on Concanavalin A imprinted and non‐imprinted cryogels were measured using a continuous system. Selective binding studies were carried out in the presence of Concanavalin B and bovine serum albumin. The selectivity studies were confirmed by fast protein liquid chromatography studies using jack bean extract from Canavalia ensiformis.