bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth
Authors: Song Yoseb , Shin Jongoh , Jin Sangrak , Lee Jung-Kul , Kim Dong Rip , Kim Sun Chang , Cho Suhyung , Cho Byung-Kwan 
Published: November 2018

Abstract
Background
Acetogenic bacteria constitute promising biocatalysts for the conversion of CO2/H2 or synthesis gas (H2/CO/CO2) into biofuels and value-added biochemicals. These microorganisms are naturally capable of autotrophic growth via unique acetogenesis metabolism. Despite their biosynthetic potential for commercial applications, a systemic understanding of the transcriptional and translational regulation of the acetogenesis metabolism remains unclear.

Results
By integrating genome-scale transcriptomic and translatomic data, we explored the regulatory logic of the acetogenesis to convert CO2 into biomass and metabolites in Eubacterium limosum. The results indicate that majority of genes associated with autotrophic growth including the Wood-Ljungdahl pathway, the reduction of electron carriers, the energy conservation system, and gluconeogenesis were transcriptionally upregulated. The translation efficiency of genes in cellular respiration and electron bifurcation was also highly enhanced. In contrast, the transcriptionally abundant genes involved in the carbonyl branch of the Wood-Ljungdahl pathway, as well as the ion-translocating complex and ATP synthase complex in the energy conservation system, showed decreased translation efficiency. The translation efficiencies of genes were regulated by 5′UTR secondary structure under the autotrophic growth condition.

Conclusions
The results illustrated that the acetogenic bacteria reallocate protein synthesis, focusing more on the translation of genes for the generation of reduced electron carriers via electron bifurcation, rather than on those for carbon metabolism under autotrophic growth.
EIF4A3-induced circular RNA MMP9 (circMMP9) acts as a sponge of miR-124 and promotes glioblastoma multiforme cell tumorigenesis
Authors: Wang Renjie , Zhang Sai , Chen Xuyi , Li Nan , Li Jianwei , Jia Ruichao , Pan Yuanqing , Liang Haiqian 
Published: November 2018

Abstract

Background
Circular RNAs (circRNAs) have been found to play critical roles in the development and progression of various cancers. However, little is known about the effects of the circular RNA network on glioblastoma multiforme (GBM).

Methods
A microarray was used to screen circRNA expression in GBM. Quantitative real-time PCR was used to detect the expression of circMMP9. GBM cells were transfected with a circMMP9 overexpression vector or siRNA, and cell proliferation, migration and invasion, as well as tumorigenesis in nude mice, were assessed to examine the effect of circMMP9 in GBM. Biotin-coupled miRNA capture, fluorescence in situ hybridization and luciferase reporter assays were conducted to confirm the relationship between circMMP9 and miR-124.

Results
In this study, we screened differentially expressed circRNAs and identified circMMP9 in GBM. We found that circMMP9 acted as an oncogene, was upregulated in GBM and promoted the proliferation, migration and invasion abilities of GBM cells. Next, we verified that circMMP9 served as a sponge that directly targeted miR-124; circMMP9 accelerated GBM cell proliferation, migration and invasion by targeting miR-124. Furthermore, we found that cyclin-dependent kinase 4 (CDK4) and aurora kinase A (AURKA) were involved in circMMP9/miR-124 axis-induced GBM tumorigenesis. Finally, we found that eukaryotic initiation factor 4A3 (eIF4A3), which binds to the MMP9 mRNA transcript, induced circMMP9 cyclization and increased circMMP9 expression in GBM.

Conclusions
Our findings indicate that eIF4A3-induced circMMP9 is an important underlying mechanism in GBM cell proliferation, invasion and metastasis through modulation of the miR-124 signaling pathway, which could provide pivotal potential therapeutic targets for the treatment of GBM.

Graphical abstract
Economic evaluation of mindfulness group therapy for patients with depression, anxiety, stress and adjustment disorders compared with treatment as usual
Authors: Saha Sanjib , Jarl Johan , Gerdtham Ulf-G , Sundquist Kristina , Sundquist Jan 
Published: November 2018

BackgroundA randomised controlled trial found that a structured mindfulness group therapy (MGT) programme was as effective as treatment as usual (mostly cognitive–behavioural therapy) for patients with a diagnosis of depression, anxiety or stress and adjustment disorders in Sweden (ClinicalTrials.gov: NCT01476371).AimsTo perform a cost-effectiveness analysis of MGT compared with treatment as usual from both a healthcare and a societal perspective for the trial duration (8 weeks).MethodThe costs from a healthcare perspective included treatment as usual, medication and costs for providing MGT. The societal perspective included costs from the healthcare perspective plus savings from productivity gains for the trial duration. The effectiveness was measured as quality-adjusted life-years (QALY) using the EQ-5D-5L questionnaire and the UK value set. Uncertainty surrounding the incremental costs and effects were estimated using non-parametric bootstrapping with 5000 replications and presented with 95% confidence intervals and cost-effectiveness acceptability curves.ResultsThe MGT group had significantly lower healthcare and societal costs (mean differences –€115 (95% CI −193 to −36) and –€112 (95% CI −207 to −17), respectively) compared with the control group. In terms of effectiveness, there was no significant difference in QALY gain (mean difference −0.003, 95% CI −0.0076 to 0.0012) between the two groups.ConclusionsMGT is a cost-saving alternative to treatment as usual over the trial duration from both a healthcare and a societal perspective for patients with a diagnosis of depression, anxiety or stress and adjustment disorders in Sweden.
Impact of Route of Estrogen Administration on Bone Turnover Markers in Oligoamenorrheic Athletes and Its Mediators
Authors: Singhal Vibha , Ackerman Kathryn E , Bose Amita , Flores Landy Paola Torre , Lee Hang , Misra Madhusmita 
Published: November 2018

Abstract

Objective
Transdermal, but not oral, estrogen replacement improves bone mineral density (BMD) in athletes with oligoamenorrhea (OA). Our objective was to determine mechanisms that may explain the impact of route of estrogen administration on bone outcomes.

Methods
Seventy-three participants with OA between 14 and 25 years old received (i) a 17β-estradiol transdermal patch continuously with cyclic oral micronized progesterone (PATCH), (ii) a combined ethinyl estradiol and desogestrel pill (PILL), or (iii) no estrogen/progesterone (NONE) for 12 months. We evaluated morning fasting levels of a marker of bone formation [N-terminal propeptide of type 1 procollagen (P1NP)], a marker of bone resorption (N-telopeptide), IGF-1, insulinlike growth factor binding protein 3, total testosterone, estradiol, SHBG, sclerostin, preadipocyte factor-1 (Pref-1), brain-derived neurotrophic factor (BDNF), calcium, 25(OH) vitamin D, and PTH levels at baseline and 12 months.

Results
Groups did not differ for age, weight, exercise activity, or markers of bone formation at baseline. Over 12 months, P1NP decreased the most in the PILL group (P = 0.03) associated with a decrease in IGF-1 levels (r = 0.37; P = 0.003). Sclerostin, Pref-1, and BDNF decreased in the PATCH group over 12 months. PATCH had the greatest increases in estradiol (P ≤ 0.0001), and estradiol increases were associated with increases in bone density.

Conclusion
Transdermal 17β-estradiol given over 12 months does not cause the decrease in IGF-1 observed with oral ethinyl estradiol. It also leads to decreases in sclerostin, Pref-1, and BDNF, which may mediate the beneficial effects of estrogen.
Conserved regulatory state expression controlled by divergent developmental gene regulatory networks in echinoids
Authors: Erkenbrack Eric M. , Davidson Eric H. , Peter Isabelle S. 
Published: November 2018

ABSTRACT
Evolution of the animal body plan is driven by changes in developmental gene regulatory networks (GRNs), but how networks change to control novel developmental phenotypes remains, in most cases, unresolved. Here, we address GRN evolution by comparing the endomesoderm GRN in two echinoid sea urchins, Strongylocentrotus purpuratus and Eucidaris tribuloides, with at least 268 million years of independent evolution. We first analyzed the expression of twelve transcription factors and signaling molecules of the S. purpuratus GRN in E. tribuloides embryos, showing that orthologous regulatory genes are expressed in corresponding endomesodermal cell fates in the two species. However, perturbation of regulatory genes revealed that important regulatory circuits of the S. purpuratus GRN are significantly different in E. tribuloides. For example, mesodermal Delta/Notch signaling controls exclusion of alternative cell fates in E. tribuloides but controls mesoderm induction and activation of a positive feedback circuit in S. purpuratus. These results indicate that the architecture of the sea urchin endomesoderm GRN evolved by extensive gain and loss of regulatory interactions between a conserved set of regulatory factors that control endomesodermal cell fate specification.
Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise
Authors: Hsu W-B. , Hsu W-H. , Hung J-S. , Shen W-J. , Hsu R. W-W. 
Published: November 2018

ObjectivesOsteoporosis is a metabolic disease resulting in progressive loss of bone mass as measured by bone mineral density (BMD). Physical exercise has a positive effect on increasing or maintaining BMD in postmenopausal women. The contribution of exercise to the regulation of osteogenesis in osteoblasts remains unclear. We therefore investigated the effect of exercise on osteoblasts in ovariectomized mice.MethodsWe compared the activity of differentially expressed genes of osteoblasts in ovariectomized mice that undertook exercise (OVX+T) with those that did not (OVX), using microarray and bioinformatics.ResultsMany inflammatory pathways were significantly downregulated in the osteoblasts after exercise. Meanwhile, IBSP and SLc13A5 gene expressions were upregulated in the OVX+T group. Furthermore, in in vitro assay, IBSP and SLc13A5 mRNAs were also upregulated during the osteogenic differentiation of MC3T3-E1 and 7F2 cells.ConclusionThese findings suggest that exercise may not only reduce the inflammatory environment in ovariectomized mice, indirectly suppressing the overactivated osteoclasts, but may also directly activate osteogenesis-related genes in osteoblasts. Exercise may thus prevent the bone loss caused by oestrogen deficiency through mediating the imbalance between the bone resorptive activity of osteoclasts and the bone formation activity of osteoblasts. Cite this article: W-B. Hsu, W-H. Hsu, J-S. Hung, W-J. Shen, R. W-W. Hsu. Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise. Bone Joint Res 2018;7:601–608. DOI: 10.1302/2046-3758.711.BJR-2018-0075.R2.
Political TV interviews in Austria 1981–2016 – Structures and strategies through times of substantial change in media and politics
Authors: Riedl Andreas 
Published: November 2018

Abstract
In media-centered democracies, political TV interviews can reveal a lot about the relationship between journalists and politicians. However, knowledge about these formats during non-election times is lacking. Against this background, this study aims to generate insights about specific conversation strategies, the staging of politics, and agenda control in a long-term comparison, and to link them with media logic, which has been identified as a factor that shapes agenda-setting strategies in related contexts. Following a static-dynamic approach, a quantitative content analysis was conducted for all statements (N=19,108) from 125 episodes of a specific Austrian interview format in a one-on-one setting broadcast between 1981 and 2016. Regarding the number of statements in which a newly introduced topic is discussed as a measure for agenda control, specific conversation strategies were identified that contribute to a stronger position for journalists. Moreover, specific aspects of media logic help to introduce and anchor a new topic, although their success varies over the decades.
Locally conformal symplectic structures on Lie algebras of type I and their solvmanifolds
Authors: Origlia Marcos 
Published: November 2018

Abstract
We study Lie algebras of type I, that is, a Lie algebra

𝔤

{\mathfrak{g}}

where all the eigenvalues of the operator

ad
X

{\operatorname{ad}_{X}}

are imaginary for all

X
∈
𝔤

{X\in\mathfrak{g}}

. We prove that the Morse–Novikov cohomology of a Lie algebra of type I is trivial for any closed 1-form. We focus on locally conformal symplectic structures (LCS) on Lie algebras of type I. In particular, we show that for a Lie algebra of type I any LCS structure is of the first kind. We also exhibit lattices for some 6-dimensional Lie groups of type I admitting left invariant LCS structures in order to produce compact solvmanifolds equipped with an invariant LCS structure.
Petrology and dating of the Permian lamprophyres from the Malá Fatra Mts. (Western Carpathians, Slovakia)
Authors: Spišiak Ján , Vetráková Lucia , Chew David , Ferenc Štefan , Mikuš Tomáš , Šimonová Viera , Bačík Peter 
Published: November 2018

Abstract
Calc–alkaline lamprophyres are known from several localities in the Malá Fatra Mountains. They form dykes (0.5–3 m) of varying degree of alteration that have intruded the surrounding granitoid rocks which are often incorporated xenoliths. Clinopyroxenes (diopside to augite), amphiboles (kaersutitic), biotites (annite) and plagioclases are major primary minerals of the dykes, accessory minerals include apatite, ilmenite, rutile, pyrite, chalcopyrite, and pyrrhotite. Apatite has a relatively low F, but increased Cl content compared to typical apatite from lamprophyres or magmatic apatite from granitic rocks in the Western Carpathians. The chemical composition of the lamprophyres indicates their calc–alkaline character, but affinity to alkaline lamprophyres is suggested by the Ti enrichment in clinopyroxene, amphibole and biotite. According to modal classification of the minerals, the studied rocks correspond to spessartite. The differences in the chemical composition of the rocks (including Sr and Nd isotopes) probably result from the contamination of primary magma by crustal material during magma ascent. The age of the lamprophyres, based on U/Pb dating in apatite, is 263.4 ± 2.6 Ma.
Accessory minerals and evolution of tin-bearing S-type granites in the western segment of the Gemeric Unit (Western Carpathians)
Authors: Broska Igor , Kubiš Michal 
Published: November 2018

Abstract
The S-type accessory mineral assemblage of zircon, monazite-(Ce), fluorapatite and tourmaline in the cupolas of Permian granites of the Gemeric Unit underwent compositional changes and increased variability and volume due to intensive volatile flux. The extended S-type accessory mineral assemblage in the apical parts of the granite resulted in the formation of rare-metal granites from in-situ differentiation and includes abundant tourmaline, zircon, fluorapatite, monazite-(Ce), Nb–Ta–W minerals (Nb–Ta rutile, ferrocolumbite, manganocolumbite, ixiolite, Nb–Ta ferberite, hübnerite), cassiterite, topaz, molybdenite, arsenopyrite and aluminophosphates. The rare-metal granites from cupolas in the western segment of the Gemeric Unit represent the topaz–zinnwaldite granites, albitites and greisens. Zircon in these evolved rare-metal Li–F granite cupolas shows a larger xenotime-(Y) component and heterogeneous morphology compared to zircons from deeper porphyritic biotite granites. The zircon Zr/Hfwt ratio in deeper rooted porphyritic granite varies from 29 to 45, where in the differentiated upper granites an increase in Hf content results in a Zr/Hfwt ratio of 5. The cheralite component in monazite from porphyritic granites usually does not exceed 12 mol. %, however, highly evolved upper rare-metal granites have monazites with 14 to 20 mol. % and sometimes > 40 mol. % of cheralite. In granite cupolas, pure secondary fluorapatite is generated by exsolution of P from P-rich alkali feldspar and high P and F contents may stabilize aluminophosphates. The biotite granites contain scattered schorlitic tourmaline, while textural late-magmatic tourmaline is more alkali deficient with lower Ca content. The differentiated granites contain also nodular and dendritic tourmaline aggregations. The product of crystallization of volatile-enriched granite cupolas are not only variable in their accessory mineral assemblage that captures high field strength elements, but also in numerous veins in country rocks that often contain cassiterite and tourmaline. Volatile flux is documented by the tetrad effect via patterns of chondrite normalized REEs (T1,3 value 1.46). In situ differentiation and tectonic activity caused multiple intrusive events of fluid-rich magmas rich in incompatible elements, resulting in the formation of rare-metal phases in granite roofs. The emplacement of volatile-enriched magmas into upper crustal conditions was followed by deeper rooted porphyritic magma portion undergoing second boiling and re-melting to form porphyritic granite or granite-porphyry during its ascent.
Representations of Chinese Language Learning in Contemporary English-language News Media: Hope, Hype, and Fear
Authors: Duff Patricia A. , Anderson Tim , Doherty Liam , Wang Rachel 
Published: November 2018

Abstract
The growing body of research on Chinese as an international (or “global”) language examines linguistic, psycholinguistic, social-psychological, and orthographic aspects of acquisition primarily. There has been relatively little critical discussion or analysis of the larger social context and discourses in which Chinese language education is embedded. However, recently sociocultural, discursive, and critical aspects of the teaching, learning, and use of Chinese as an additional language have begun to receive more attention. This study analyzes circulating discourses, ideologies, and tropes related to Chinese in news media, as one means by which information and perspectives are spread by media and by which public attitudes and policy decisions are (recursively) shaped or reproduced. To this end, a large sample of English-medium news reports of Chinese language education in three Anglophone countries was created and analyzed for the years 2004 to 2012.
The findings revealed that reports dealing with Chinese education tended to fall into one of several major tropes, which we have roughly classified as “hope,” “hype,” and “fear,” distinctions that parallel existing models of cyclical or amplified media coverage of innovations or otherwise newsworthy events. The sociopolitically and socioeconomically motivated occurrence of these tropes in the media, combined with the novelty of the Chinese language itself, a historically less frequently taught language in comparison with various European languages, constituted a consistent and recurring narrative. Thus, the shifting representations of Chinese learning in the media tended to appear as corollaries or “side stories” servicing the needs of larger geopolitical events and perceived or desired changes in public sentiment. These trends and their significance are illustrated and discussed in relation to Global Chinese.
Speaking Chinese: A major Challenge in the Construction of Identity and the Preservation of the Peruvian Chinese Community (1870–1930)
Authors: Lausent-Herrera Isabelle 
Published: November 2018

Abstract
The arrival of around 100,000 Chinese male workers, in Peru between 1849 and 1874 as indentured labor created particularly difficult conditions for the emergence and development of a Chinese community. Arriving without women, the majority of the Chinese founded families in Peru. To conserve a blood link with their Chinese identity, many sought to marry young mixed blood Chinese-Peruvian girls. However, to make up a Chinese community, a Chinese education was considered essential for the transmission and preservation of cultural values and language. There were several attempts to create a Chinese school for the children of the Emperor’s subjects, first by the church in 1882 and later by Chinese officials as early as 1885, following the model of San Francisco and Havana. This article examines the historical development between 1870 and 1930 of the efforts the Chinese community in Peru made in setting up Chinese language education and community associations and the institutions that supported the development.
Virtual communities: Interaction, identity and authority in digital communication
Authors: Reyes Antonio 
Published: November 2018

Abstract
This paper analyzes two settings of digital communication in which social actors participate, interact and engage differently within virtual communities. Online identities are interactionally and discursively constructed and this process defines differences in groupness, illustrating the nature of those online spaces. This study builds on the notion of Citizen Sociolinguistics, that is, ordinary people producing metacommentaries on language uses, to examine the data in different digital communication settings.
This work analyzes 500 comments posted by readers on the site of the newspaper El País, in response to an article announcing the new Spanish orthographic reforms. Additionally, it analyzes 200 threads of the “Sólo español” (“Spanish only”) sub-forum within WordReference.com. The analysis investigates identity and hierarchy in digital communication as socio-cultural results of linguistic interaction. By analyzing interaction and identity in online spaces, this paper proposes empirical ways to account for differences in virtual communities, reconsiders established criteria such as time and regularity in interaction, and proposes new criteria (hierarchy and authority) to accentuate the distinctions regarding virtual communities.