bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of optic disc-fovea distance on macular thickness measurements with OCT in healthy myopic eyes
Authors: Qiu Kunliang , Wang Geng , Zhang Riping , Lu Xuehui , Zhang Mingzhi , Jansonius Nomdo M. 
Published: March 2018

AbstractAssessment of macular thickness is important in the evaluation of various eye diseases. This study aimed to determine the influence of the optic disc-fovea distance (DFD) on macular thickness in myopic eyes. We determined the DFD and the macular thickness in 138 eyes from 138 healthy myopic subjects using the Cirrus HD-OCT. Correlation analysis and multiple linear regression were performed to determine the influence of DFD, axial length, disc area, and β-PPA on macular thickness. To further remove the confounding effect of ocular magnification on the DFD and OCT scan area, a subgroup analysis was performed in eyes with a limited axial length range (24–25 mm). DFD was significantly correlated with both regional (central, inner, and outer ETDRS subfields) and overall average macular thickness at a Bonferroni corrected P value of 0.004 (r ranging from −0.27 to −0.47), except for the temporal outer (r = −0.15, P = 0.089) and inferior outer (r = −0.22, P = 0.011) macular thickness. In the multivariable analysis, DFD was significantly associated with the average inner and outer macular thickness, the central subfield thickness, and the overall macular thickness (all P < 0.001), independent of ocular magnification and other covariates. Our findings indicate that eyes with a greater DFD have a lower macular thickness.
First detection of human hepegivirus-1 (HHpgV-1) in Iranian patients with hemophilia
Authors: Bijvand Yazdan , Aghasadeghi Mohammad Reza , Sakhaee Fatemeh , Pakzad Parviz , Vaziri Farzam , Saraji Alireza Azizi , Jamnani Fatemeh Rahimi , Siadat Seyed Davar , Fateh Abolfazl 
Published: March 2018

AbstractA novel blood-borne virus called the human hepegivirus 1 (HHpgV-1) was recently discovered in hemophilia patients. The present study aimed to investigate the presence of HHpgV-1 in hemophilia patients. A total of 436 serum samples were investigated for the presence of hepatitis C virus (HCV), human pegivirus-1 (HPgV-1), torque teno virus (TTV), and HHpgV-1. Out of the 436 patients, 163 (37.4%), 19 (4.4%), 76 (17.4%), and four (0.9%) patients were positive for HCV, HPgV-1, TTV, and HHpgV-1, respectively. HHpgV-1 patients had a mean viral load of 4.9 ± 0.3 log RNA copies/mL and were co-infected with HCV-1a, HPgV-1, and TTV. Moreover, three HHpgV-1-positive patients exhibited stage F0 liver fibrosis. HCV viral load in HHpgV-1-positive patients was lower than those of HHpgV-1-negative patients. Results also revealed that co-infection of HHpgV-1 with HPgV-1 and HCV may play a protective role in patients with chronic HCV. In conclusion, we detected a low frequency of HHpgV-1 infection in hemophilia patients, and results suggested that HHpgV-1 infection was correlated with the presence of other blood-borne viruses and is likely to also correlate with low HCV viral load and reduced severity of liver disease. Additional studies are required to further investigate the clinical importance of HHpgV-1.
Sex Genotyping of Archival Fixed and Immunolabeled Guinea Pig Cochleas
Authors: Depreux Frédéric F. , Czech Lyubov , Whitlon Donna S. 
Published: March 2018

AbstractFor decades, outbred guinea pigs (GP) have been used as research models. Various past research studies using guinea pigs used measures that, unknown at the time, may be sex-dependent, but from which today, archival tissues may be all that remain. We aimed to provide a protocol for sex-typing archival guinea pig tissue, whereby past experiments could be re-evaluated for sex effects. No PCR sex-genotyping protocols existed for GP. We found that published sequence of the GP Sry gene differed from that in two separate GP stocks. We used sequences from other species to deduce PCR primers for Sry. After developing a genomic DNA extraction for archival, fixed, decalcified, immunolabeled, guinea pig cochlear half-turns, we used a multiplex assay (Y-specific Sry; X-specific Dystrophin) to assign sex to tissue as old as 3 years. This procedure should allow reevaluation of prior guinea pig studies in various research areas for the effects of sex on experimental outcomes.
Bile Acids Increase Doxorubicin Sensitivity in ABCC1-expressing Tumour Cells
Authors: Chewchuk Simon , Boorman Tyler , Edwardson Derek , Parissenti Amadeo M. 
Published: March 2018

AbstractTumour cells possess or acquire various mechanisms to circumvent the cytotoxic effects of chemotherapy drugs. One such mechanism involves the overexpression of ABC transporters that facilitate the extrusion of a variety of structurally distinct chemotherapy drugs from the cytoplasm into the extracellular space. While specific ABC transporter inhibitors have been developed, many affect other ABC transporters, particularly at elevated concentrations. It is also unclear whether they show clear efficacy for combatting drug resistance in cancer patients with minimal host toxicity. In this study, we demonstrate the ability of two bile acids [β-cholanic acid (urso-cholanic acid) and deoxycholic acid] to specifically inhibit ABCC1-mediated drug transport, augmenting doxorubicin accumulation in breast and lung tumour cells selected for doxorubicin resistance through overexpression of the ABCC1 (but not ABCB1) drug transporter. The bile acids could also restore uptake and sensitivity to doxorubicin in human endothelial kidney cells genetically engineered to overexpress the ABCC1 drug transporter. These observations suggest a previously unreported role for bile acids as ABCC1 inhibitors or regulators. Given its additional properties of minimal clinical toxicity in humans and its ability to inhibit aldo-keto reductases involved in anthracycline resistance and anthracycline-induced cardiotoxicity, β-cholanic acid merits further in vivo and clinical investigation.
Asymmetries in Distractibility: Left Distractors Improve Reaction Time Performance
Authors: Thomas Nicole A. , Nicholls Michael E. R. 
Published: March 2018

AbstractResearch using the irrelevant-distractor paradigm shows perceptual load influences distractibility, such that distractors are more likely to be processed and decrease reaction times during low perceptual load. In contrast, under high load, attentional resources are limited, and the likelihood of distractibility is decreased. We manipulated distractor placement to determine whether location differentially influenced distractibility. During low load, reaction times were increased equally for all distractor locations. Under high load, left distractors speeded reaction times significantly more than right distractors. We suggest two potential explanations: (1) the central focus of attention was sufficiently large to encapsulate both the distractor and the visual array during low perceptual load, leading to increased distraction—during high load, attention was split across the two visual stimuli, allowing the distractors and array to be processed independently; (2) superior executive control for stimuli in the left visual field allowed participants to ‘catch and release’ left distractors more efficiently, ultimately decreasing distraction and providing a performance benefit. Our findings represent an intriguing development in relation to visual asymmetries in distractibility.
Hybrid graphene/cadmium-free ZnSe/ZnS quantum dots phototransistors for UV detection
Authors: Sun Yi-Lin , Xie Dan , Sun Meng-Xing , Teng Chang-Jiu , Qian Liu , Chen Ruo-Song , Xiang Lan , Ren Tian-Ling 
Published: March 2018

AbstractGraphene-based optoelectronic devices have attracted much attention due to their broadband photon responsivity and fast response time. However, the performance of such graphene-based photodetectors is greatly limited by weak light absorption and low responsivity induced by the gapless nature of graphene. Here, we achieved a high responsivity above 103 AW−1 for Ultraviolet (UV) light in a hybrid structure based phototransistor, which consists of CVD-grown monolayer graphene and ZnSe/ZnS core/shell quantum dots. The photodetectors exhibit a selective photo responsivity for the UV light with the wavelength of 405 nm, confirming the main light absorption from QDs. The photo-generated charges have been found to transfer from QDs to graphene channel, leading to a gate-tunable photo responsivity with the maximum value obtained at V
G
about 15V. A recirculate 100 times behavior with a good stability of 21 days is demonstrated for our devices and another flexible graphene/QDs based photoconductors have been found to be functional after 1000 bending cycles. Such UV photodetectors based on graphene decorated with cadmium-free ZnSe/ZnS quantum dots offer a new way to build environmental friendly optoelectronics.
Proteins involved in embryo-maternal interaction around the signalling of maternal recognition of pregnancy in the horse
Authors: Smits Katrien , Willems Sander , Van Steendam Katleen , Van De Velde Margot , De Lange Valérie , Ververs Cyrillus , Roels Kim , Govaere Jan , Van Nieuwerburgh Filip , Peelman Luc , Deforce Dieter , Van Soom Ann 
Published: March 2018

AbstractDuring maternal recognition of pregnancy (MRP), a conceptus-derived signal leads to the persistence of the corpus luteum and the maintenance of gestation. In the horse, the nature of this signal remains to be elucidated. Several studies have focused on the changes in gene expression during MRP, but little information exists at the protein level. The aim of this study was to identify the proteins at the embryo-maternal interface around signalling of MRP in the horse (day 13) by means of mass spectrometry. A distinct influence of pregnancy was established, with 119 proteins differentially expressed in the uterine fluid of pregnant mares compared to cyclic mares and with upregulation of several inhibitors of the prostaglandin synthesis during pregnancy. By creating an overview of the proteins at the embryo-maternal interface in the horse, this study provides a solid foundation for further targeted studies of proteins potentially involved in embryo-maternal interactions, MRP and pregnancy loss in the horse.
Transcriptome-wide identification and screening of WRKY factors involved in the regulation of taxol biosynthesis in Taxus chinensis
Authors: Zhang Meng , Chen Ying , Nie Lin , Jin Xiaofei , Liao Weifang , Zhao Shengying , Fu Chunhua , Yu Longjiang 
Published: March 2018

AbstractWRKY, a plant-specific transcription factor family, plays important roles in pathogen defense, abiotic cues, phytohormone signaling, and regulation of plant secondary metabolism. However, little is known about the roles, functions, and mechanisms of WRKY in taxane biosynthesis in Taxus spp. In this study, 61 transcripts were identified from Taxus chinensis transcriptome datasets by using hidden Markov model search. All of these transcripts encoded proteins containing WRKY domains, which were designated as TcWRKY1–61. After phylogenetic analysis of the WRKY domains of TcWRKYs and AtWRKYs, 16, 8, 10, 14, 5, 7, and 1 TcWRKYs were cladded into Group I, IIa–IIe, and III, respectively. Then, six representative TcWRKYs were selected to classify their effects on taxol biosynthesis. After MeJA (methyl jasmonate acid) and SA (salicylic acid) treatments, all of the six TcWRKYs were upregulated by MeJA treatment. TcWRKY44 (IId) and TcWRKY47 (IIa) were upregulated, whereas TcWRKY8 (IIc), TcWRKY20 (III), TcWRKY26 (I), TcWRKY41 (IIe), and TcWRKY52 (IIb) were downregulated by SA treatment. Overexpression experiments showed that the six selected TcWRKYs exerted different effects on taxol biosynthesis. In specific, TcWRKY8 and TcWRKY47 significantly improved the expression levels of taxol-biosynthesis-related genes. Transcriptome-wide identification of WRKY factors in Taxus not only enhances our understanding of plant WRKY factors but also identifies candidate regulators of taxol biosynthesis.
Discovery of a highly selective JAK3 inhibitor for the treatment of rheumatoid arthritis
Authors: Pei Heying , He Linhong , Shao Mingfeng , Yang Zhuang , Ran Yan , Li Dan , Zhou Yuanyuan , Tang Minghai , Wang Taijin , Gong Yanqiu , Chen Xiaoxin , Yang Shengyong , Xiang Mingli , Chen Lijuan 
Published: March 2018

AbstractJanus tyrosine kinase 3 (JAK3) is expressed in lymphoid cells and is involved in the signalling of T cell functions. The development of a selective JAK3 inhibitor has been shown to have a potential benefit in the treatment of autoimmune disorders. In this article, we developed the 4-aminopiperidine-based compound RB1, which was highly selective for JAK3 inhibition, with an IC50 of value of 40 nM, but did not inhibit JAK1, JAK2 or tyrosine kinase 2 (TYK2) at concentrations up to 5 µM. Furthermore, RB1 also exhibited favourable selectivity against a panel of representative kinases. In a battery of cytokine-stimulated cell-based assays, this potent inhibitor of JAK3 activity with good selectivity against other kinases could potently inhibit JAK3 activity over the activity of JAK1 or JAK2 (over at least 100-fold). A combination of liquid chromatography-mass spectrometry (LC-MS) experiments validated that RB1 covalently modified the unique cysteine 909 residue in JAK3. In vivo, RB1 exerted significantly improved pathology in the joints of a collagen-induced arthritis mouse model. The reasonable pharmacokinetics properties (F = 72.52%, T1/2 = 14.6 h) and favourable results of toxicology experiments (LD50 > 2 g/kg) suggest that RB1 has the potential to be an efficacious treatment for RA.
Maresin-1 suppresses imiquimod-induced skin inflammation by regulating IL-23 receptor expression
Authors: Saito-Sasaki Natsuko , Sawada Yu , Mashima Emi , Yamaguchi Takashi , Ohmori Shun , Yoshioka Haruna , Haruyama Sanehito , Okada Etsuko , Nakamura Motonobu 
Published: March 2018

AbstractThe anti-inflammatory effect of omega 3 polyunsaturated fatty acids has been confirmed in various inflammatory disease models. Maresin-1 (MaR1) is a lipid mediator derived from the omega-3 fatty acid docosahexaenoic acid (DHA) that has displayed strong anti-inflammatory effects in various inflammatory disease models. However, the effect of topical MaR1 on cutaneous inflammation remains unclear. Therefore, we initially examined the anti-inflammatory effects of topical Maresin-1 using an imiquimod (IMQ)-induced psoriasis-like mouse model of inflammation. Topical MaR1 reduced the ear swelling response as seen in histological findings. RT-PCR and flow cytometry analyses revealed MaR1 had no inhibitory effect on IL-23, but MaR1 suppressed IL-17A production by γδTCRmid+ and CD4+ cells in the skin. These inhibitory effects were also observed in a subcutaneous IL-23-injected psoriasis model. MaR1 downmodulated IL-23 receptor (IL-23R) expression by suppressing retinoic acid-related orphan receptor γt (RORγt) expression and internalization in a clathrin-dependent manner in γδTCRmid+ and CD4+ cells. These results lead to assumptions that topical MaR1 may be a new therapeutic agent for psoriasis and other IL-17-mediated cutaneous inflammatory diseases.
Early clinical experience with Guidezilla for transradial interventions in China
Authors: Ma Min , Diao Kai-yue , Liu Xiao-jing , He Yong 
Published: March 2018

AbstractAnatomic variations, calcified, tortuous, angulated lesions, and lack of support to increase the complexity of transradial intervention (TRI). Guidezilla is a mother-and-child catheter enabling increased support during complex interventions. As there are few published reports of its use, we describe our experience using this device to assist TRI in Chinese patients. The aim of this study was to investigate the efficacy and safety of the Guidezilla guide extension catheter in complex coronary interventions. Thirty-two patients’ clinical characteristics, angiographic details, and in-hospital outcome retrospectively collected between June 2015 and August 2017. Patients were 59.44 ± 10.48 years of age and 26 (81%) were men. The most frequent target vessels were the RCA (34%) and LAD (31%), patients had complex type C (53%) or B (47%) lesions, severely tortuous (41%) and angulated (22%).With the use of Guidezilla, technique success was 100%, and procedural success was 94%. The mean diameter of the deployed stents was 2.97 ± 0.37 mm, and the length was 27.19 ± 8.14 mm. The estimated mean distance of Guidezilla intubation into the target vessel was 7.66 ± 2.29 cm.The Guidezilla catheter extension safely facilitated successful completion of TRI in complex coronary artery lesions. This device can help interventionalists successfully perform difficult procedures.
Elucidation of the bacterial communities associated with the harmful microalgae
Alexandrium tamarense
and
Cochlodinium polykrikoides
using nanopore sequencing
Authors: Shin HyeonSeok , Lee Eunju , Shin Jongoh , Ko So-Ra , Oh Hyung-Seok , Ahn Chi-Yong , Oh Hee-Mock , Cho Byung-Kwan , Cho Suhyung 
Published: March 2018

AbstractInteractions between microalgae and bacteria are often obligatory for harmful algal blooms (HABs). Here, we investigated the specific bacterial communities associated with Alexandrium tamarense and Cochlodinium polykrikoides, which cause ecological and economic damage during their blooms. To this end, the bacterial metagenome was selectively isolated from the two dinoflagellates and subsequently used for 16S rRNA analysis via the Nanopore MinION and Illumina sequencing platforms. Although the full-length 16S rRNA reads from the MinION platform showed high correlation in higher taxonomic ranks to the partial-length 16S rRNA reads from the Illumina platform, there was less correlation at the genus and species levels. MinION reads that are similar in the V3-V4 hypervariable regions with Illumina reads are classified to different taxonomies due to the extra information encoded in the full-length 16S rRNA reads. This indicates that bias arising from the short length Illumina reads can be supplemented by MinION reads. Furthermore, integrated analysis of the Illumina and MinION data showed that A. tamarense was predominantly enriched in the Roseobacter clade and C. polykrikoides was enriched in Gammaproteobacteria and Alphaproteobacteria. These results suggest that the association of different bacterial communities with A. tamarense and C. polykrikoides may be required for HABs.
Topological estimation of signal flow in complex signaling networks
Authors: Lee Daewon , Cho Kwang-Hyun 
Published: March 2018

AbstractIn a cell, any information about extra- or intra-cellular changes is transferred and processed through a signaling network and dysregulation of signal flow often leads to disease such as cancer. So, understanding of signal flow in the signaling network is critical to identify drug targets. Owing to the development of high-throughput measurement technologies, the structure of a signaling network is becoming more available, but detailed kinetic parameter information about molecular interactions is still very limited. A question then arises as to whether we can estimate the signal flow based only on the structure information of a signaling network. To answer this question, we develop a novel algorithm that can estimate the signal flow using only the topological information and apply it to predict the direction of activity change in various signaling networks. Interestingly, we find that the average accuracy of the estimation algorithm is about 60–80% even though we only use the topological information. We also find that this predictive power gets collapsed if we randomly alter the network topology, showing the importance of network topology. Our study provides a basis for utilizing the topological information of signaling networks in precision medicine or drug target discovery.
Polarized electron beams elastically scattered by atoms as a tool for testing fundamental predictions of quantum mechanics
Authors: Dapor Maurizio 
Published: March 2018

AbstractQuantum information theory deals with quantum noise in order to protect physical quantum bits (qubits) from its effects. A single electron is an emblematic example of a qubit, and today it is possible to experimentally produce polarized ensembles of electrons. In this paper, the theory of the polarization of electron beams elastically scattered by atoms is briefly summarized. Then the POLARe program suite, a set of computer programs aimed at the calculation of the spin-polarization parameters of electron beams elastically interacting with atomic targets, is described. Selected results of the program concerning Ar, Kr, and Xe atoms are presented together with the comparison with experimental data about the Sherman function for low kinetic energy of the incident electrons (1.5eV–350eV). It is demonstrated that the quantum-relativistic theory of the polarization of electron beams elastically scattered by atoms is in good agreement with experimental data down to energies smaller than a few eV.
Sex-based differences in phagocyte metabolic profile in rats with monosodium glutamate-induced obesity
Authors: Rudyk Mariia P. , Pozur Valentyna V. , Voieikova Daryna O. , Hurmach Yevheniia V. , Khranovska Nataliia M. , Skachkova Oksana V. , Svyatetska Vitalina M. , Fedorchuk Olexander G. , Skivka Larysa M. , Berehova Tetiana V. , Ostapchenko Liudmyla I. 
Published: March 2018

AbstractThe important component of obesity pathogenesis is inflammatory activation of innate immune cells within adipose tissue and in other body locations. Both the course of obesity and innate immune reactivity are characterized by sex-associated differences. The aim of the work was a comparative investigation of metabolic profiles of phagocytes from different locations in male and female rats with MSG-induced obesity. The administration of monosodium glutamate (MSG) caused obesity, with sex-associated differences, that was more severe in male rats. Obesity was associated with pro-inflammatory activation of CD14+ phagocytes from adipose tissue in female, but not in male rats, which was demonstrated by decreased phagocytosis activity along with increased ROS generation. Phagocytes from the peritoneal cavity and peripheral blood of obese female rats exhibited neutral metabolic profile, whereas those cells from obese male rats displayed a pro-inflammatory metabolic profile. Thus, the manifestation of obesity-induced inflammation was characterized by different patterns of metabolic profile of phagocytes in male and female rats. Identified immune cell characteristics expand our knowledge of obesity immunobiology and may help to develop more effective preventive and therapeutic interventions for obese patients of different sexes.
Development, characterization and dissolution behavior of calcium-aluminoborate glass wasteforms to immobilize rare-earth oxides
Authors: Kim Miae , Corkhill Claire L. , Hyatt Neil C. , Heo Jong 
Published: March 2018

AbstractCalcium-aluminoborate (CAB) glasses were developed to sequester new waste compositions made of several rare-earth oxides generated from the pyrochemical reprocessing of spent nuclear fuel. Several important wasteform properties such as waste loading, processability and chemical durability were evaluated. The maximum waste loading of the CAB compositions was determined to be ~56.8 wt%. Viscosity and the electrical conductivity of the CAB melt at 1300 °C were 7.817 Pa·s and 0.4603 S/cm, respectively, which satisfies the conditions for commercial cold-crucible induction melting (CCIM) process. Addition of rare-earth oxides to CAB glasses resulted in dramatic decreases in the elemental releases of B and Ca in aqueous dissolution experiments. Normalized elemental releases from product consistency standard chemical durability test were <3.62·10−5 g·m−2 for Nd, 0.009 g·m−2 for Al, 0.067 g·m−2 for B and 0.073 g·m−2 for Ca (at 90, after 7 days, for SA/V = 2000m−1); all meet European and US regulation limits. After 20 d of dissolution, a hydrated alteration layer of ~ 200-nm-thick, Ca-depleted and Nd-rich, was formed at the surface of CAB glasses with 20 mol% Nd2O3 whereas boehmite [AlO(OH)] secondary crystalline phases were formed in pure CAB glass that contained no Nd2O3.