bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activated interferon signaling by down-regulation of CENP-N contributing to inhibited tumor growth in breast cancer
Authors: Zhai Zhenhua , Zhou Ye , Liu Xing , Wang Ying , Zhang Yuyang , Cheng Rui , Zhang Xiaomeng , Zhu Zhitu , Sun Hongzhi 
Published: August 2019

Abstract
Background Centromere proteins (CENPs) are primary components for chromosomal segregation in the mitotic stage. CENP-N is a member of CENPs, and is a key factor for recruitment of other CENPs and formation of a link between the centromere and micro-tubules, which facilitate cell division.
Methods In order to clarify the role of CENP-N in breast cancer, RNA sequences data were downloaded from TCGA online database and the CENP-N expression was knocked down in breast cancer cells.
Results The results show that the expression of CENP-N was higher in breast cancer comparing with the paracancerous tissues. In breast cancer, patients with high expression of CENP-N have a short-term overall survival compared with low expression of CENP-N. Both in vitro and in vivo, the growth of breast cancer cells was inhibited by down-regulation of CENP-N. In the gene-chip analysis, it reveals that down-regulation of CENP-N is primarily associated with functions of immune response and anti-tumor ef-fects. Of these changed canonical pathways, the activated interferon signaling was the most significant in CENP-N down-regulated breast cancer cells. In the western blot as-say, up-regulated expressions of molecules involved in interferon signaling were also confirmed.
Conclusions Our results suggest that CENP-N can be a potential therapeutic target in the treatment of breast cancer, and the involved interferon signaling needs to be mainly fo-cused on.
Keywords: CENP-N, Breast cancer, interferon signaling, Tumor growth
Screening biogenic amines and fish-based food (keropok lekor) extracts in induction of inflammation using Principal Component Analysis
Authors: Hussain Husniza , Bustamam MS Ahamad , Vimala B. , Lai Too Chun , Mahiyuddin Wan Rozita Wan , Fitrianto Anwar , Razak Mohd. Fuat Abd. , Mohamud Wan Nazaimoon Wan , Bakar Jamilah , Ghazali Hasanah Mohd 
Published: August 2019

Abstract
Background: Food-borne biogenic amines (BAs), namely, histamine, putrescine, cadaverine, tyramine, spermine and spermidine are known for their contributions as fish-based food freshness biomarkers to determine level of contamination. The remaining food–borne BAs (phenylethylamine, tryptamine and agmatine) effects on promoting inflammation are yet to be investigated. The effect of these compounds on induction of inflammation in macrophages was investigated using Principal Component Analysis (PCA) from independent (BAs at 1, 10 and 100 µg/ml, food extract and its standard mixture solution) and dependent variables [cell viability, nitric oxide (NO) and tumor necrosis factor-α (TNF-α) secretion]. Nine individual BAs and keropok lekor extracts were exposed to RAW 264.7 macrophages for 18-24 hr at 37oC with 5% carbon dioxide environment. Cell viability, NO and TNF-α secretion were determined using MTS assay kit, Greiss Reagent System and Enzyme-linked Immunosorbent Assay (ELISA) kits, respectively.
Results: Q2V values were not equal to Q2 (an estimate of the predictive ability of the model) values for individual variables because the eigenvalues values were more than 0.5, indicating a good model. All variance (R2VX) values were > 0.9, suggesting goodness of fit.
Conclusions: PCA is thus proven as an effective tool to discriminate between inflammogenic and non-inflammogenic food-borne BAs.
Low level of awareness among Iranian women concerning cervical cancer and screening: the role of knowledge, risk perceptions and cues to action
Authors: Asl Rahim Taghizadeh , Osch Liesbeth Van , De Vries Nanne , Zendehdel Kazem , Shams Mohsen , Zarei Fatemeh , De Vries Hein 
Published: August 2019

Abstract
Background

Iran has a low incidence of, but a higher rate of death from, cervical cancer (CC). The country is in the process of implementing an organized screening program including HPV testing and cytology. Studies show high drop-out in continued testing among eligible women. This qualitative study aimed to explore women’s awareness regarding CC and CC testing, and the role of knowledge, perceived risk and cues to action in this process.

Methods

We recruited 81 women aged between 25-65 years, who participated in 15 focus group discussions (FGDs) and two in-depth interviews in Tehran, the capital of Iran. Data were acquired through 11 open-ended questions and 32 related probe questions. All interviews were transcribed and independently analyzed by two researchers (Kappa and agreement testing respectively: 0.77, 97.11%).

Results

Coded texts were categorized under three themes and 13 subthemes. The three thematic areas referred to knowledge, cues to action and perceived risks regarding cervical cancer and screening. Results showed that women had inadequate and unspecified knowledge about cervical cancer and screening, compounded by misconceptions regarding infection and cancer prevention measures. Social and cultural barriers hindered proper communication between health system/providers and clients, and within communities on subjects related to cervical cancer and screening. Perceived risk of getting CC was low due to insufficient knowledge regarding the causes of cancer, difficulty in differentiating between cancer and sexually transmitted infections, and the absence of visible symptoms.

Conclusions

The results indicate a strong need to invest more efforts to improve health education and communication in the current national health program to promote awareness of the need to screen for CC, through, for example, establishing correct knowledge and risk perceptions among women. In addition, this intervention should address women’s social environment in order to prevent misconceptions being communicated to women.
Efficacy of a mindfulness-based intervention programme with and without virtual reality support to reduce stress in university students: protocol for a randomized controlled pragmatic trial
Authors: Modrego-Alarcón Marta , Navarro-Gil María Teresa , Beltrán-Ruiz María , Monreal-Bartolomé Alicia , Gascón Santiago , López-del-Hoyo Yolanda , García-Campayo Javier , Delgado-Suárez Irene , Montero-Marín Jesús 
Published: August 2019

Abstract
Background There is growing concern about mental health problems in university students. For this reason, mindfulness training is becoming increasingly popular in university settings. However, mindfulness-based interventions (MBIs) usually present high attrition rates. This trial aims to evaluate the efficacy of a MBI to reduce perceived stress and to improve the psychological well-being of university students, as well as to explore the capacity of virtual reality (VR) to enhance adherence to the intervention. Methods This study protocol presents a randomized controlled trial (RCT) involving assessment time points of baseline, posttreatment and 6-month follow-up. A total of 280 students from the Spanish National Distance Education University (UNED) or the University of Zaragoza will be randomly assigned to a mindfulness condition, a mindfulness condition complemented by VR, and a relaxation condition to serve as a control group. Stress will be the main outcome and will be measured using the 10-item self-report Perceived Stress Scale (PSS). Other well-being and academic functioning outcomes will be assessed, as well as variables that explore the impact of VR. Multilevel mixed-effects models will be calculated to estimate the efficacy of the programme, and effect size estimations will be carried out. Effects of VR in adherence to programme will be evaluated. Discussion Some strengths of this study are the RCT design, which includes a suitable active control group and a 6-month follow-up assessment; a large sample size of university students at different stages of their courses and a variety of degrees; and the incorporation of the VR support to facilitate completion of the MBI programme. Potential limitations are the voluntary participation of the students and the utilization of self-report measures. Trial registration number ClinicalTrials.gov NCT03771300.
Identification and Classification of LEA Family Genes in Orchids and Characterization of Their Role in Callus Formation
Authors: He Chunmei , Liu Xuncheng , Silva Jaime A. Teixeira da , Wang Haobin , Peng Tao , Zhang Mingze , Si Can , Yu Zhenming , Tan Jianwen , Zhang Jianxia , Luo Jianping , Duan Jun 
Published: August 2019

Abstract
The plant late embryogenesis abundant (LEA) proteins are abundant in seeds, play an important role in various abiotic stresses. However, there is still no information on genome-wide identification of LEA genes in orchids and their function in callus formation is almost unknown. In this study, the LEA genes from two orchids (Phalaenopsis equestris and Dendrobium officinale), were genome-wide identified, classified and characterized. A total of 57 and 59 LEA genes were identified in the genomes and these were divided into 8 and 9 groups for P. equestris and D. officinale, respectively. The LEA_1 and LEA_4 genes from P. equestris and D. officinale showed strong expression in seeds, but were significantly down-regulated in flowers and absent in vegetative organs (leaves, stems and roots). In addition, the LEA_1 and LEA_4 genes from D. officinale were abundant in the protocorm-like body (PLB) stage, while weak signals that were detected in in vitro shoots could not be detected in plantlets. The expression of these genes highlights PLBs in orchids are somatic embryos. The DoLEA36 from LEA_4 and DoLEA43 from LEA_1 were further characterized. The GFP signal of the DoLEA36-GFP fusion protein was only detected in the cytoplasm, while the GFP signal of the DoLEA43-GFP fusion protein was detected in both the cytoplasm and nucleus. This indicates that DoEA36 localizes in the cytoplasm while DoLEA43 localizes in both the cytoplasm and nucleus. Both DoLEA36 and DoLEA43 stimulated callus formation in transgenic Arabidopsis. The percentage of callus formation from 35S::DoLEA43 transgenic lines was higher than in wild type plants in two callus induction methods. Our results provide comprehensive information about the LEA gene family in orchids and genetic evidence for the involvement of LEA genes in the induction of callus, which may reveal their positive role in the maintenance of PLBs in orchids.
Gene expression profiling identifies pathways involved in seeds maturation of Jatropha curcas
Authors: Maghuly Fatemeh , Deak Tamas , Vierlinger Klemens , Pabinger Stephan , Tafer Hakim , Laimer Margit 
Published: August 2019

Abstract
Background: Jatropha curcas, a tropical shrub, is a promising biofuel crop, which produces seeds with a high content of oil and protein. To better understand the development of its seeds to improve Jatropha`s agronomic performance, a two-step approach was performed: 1) generation of the entire transcriptome of six different maturation stages of J. curcas seeds using 454-Roche sequencing of a cDNA library, 2) comparison of transcriptional expression levels in six different developmental stages of seeds using a custom Agilent 8x60K oligonucleotide microarray. Results: A total of 793,875 high-quality reads were assembled into 19,841 unique full-length contigs, of which 13,705 could be annotated with Gene Ontology (GO) terms. Microarray data analysis identified 9,111 probes (out of 57,842 probes), which were differentially expressed between the six developmental stages. The expression results were validated for 70 randomly selected putative genes. Result from cluster analyses showed that transcripts related to sucrose, fatty acid, flavonoid, phenylpropanoid, lignin, hormone biosynthesis were over-represented in the early stage, while lipid storage, seed dormancy and maturation in the late stage. Generally, the expression of the most over-represented transcripts decrease in the last stage of seed maturation. Further, expression analyses of different maturation stages of J. curcas seed showed that most changes in transcript abundance occurred between the two last stages, suggesting that the timing of metabolic pathways during seed maturation in J. curcas is in late stages. The co-expression result showed a high degree of connectivity between genes that play essential role in fatty acid biosynthesis and nutrient mobilization. Furthermore, seed development and hormone pathways are significantly well connected. Conclusion: The obtained results revealed DESs regulating important pathways related to seed maturation, which could contribute to understanding the complex regulatory network during seed development. This study provides detailed information on transcription changes during J. curcas seed development and provides a starting point for a genomic survey of seed quality traits. The current results highlighted specific genes and processes relevant to the molecular mechanisms involved in Jatropha seed development, and it is anticipated that this data can be delivered to other Euphorbiaceae species of economic value.
Association between echocardiographic manifestations and bacterial infections in patients with infective endocarditis: a cohort study
Authors: Damlin Anna , Westling Katarina , Maret Eva , Lundborg Cecilia Stålsby , Caidahl Kenneth , Eriksson Maria J 
Published: August 2019

Abstract
Abstract
Background
The diagnosis of infective endocarditis (IE) is based on microbiological analyses and diagnostic imaging of cardiac manifestations. Echocardiography (ECHO) is preferred for direct visualization of IE-induced cardiac manifestations. We investigated correlations between bacterial infections and IE manifestations diagnosed by ECHO.
Methods
In this cohort study, data from patients aged 18 years or above, with definite or possible IE admitted at the Karolinska University Hospital between 2008-2017 were obtained from Swedish National Registry of Endocarditis. Bacteria registered as pathogen were primarily selected from positive blood culture and for patients with negative blood culture, bacteria found in culture or PCR from postoperative material was registered as pathogen. Patients with negative results from culture or PCR were excluded. IE manifestations diagnosed by ECHO and risk factors were obtained from the registry. Chi-squared test and two-sided Fisher’s exact test was used for comparisons between categorical variables, and student’s ttest was used for continuous numerical variables; two-sided and skewed variables were log-transformed before these analyses. Multivariable analyses were performed using logistic regression. Associations and the strength between the variables were estimated using odds ratios (ORs) with 95% confidence intervals (CIs). P< 0.05 was considered significant.
Results
The most common bacteria were Staphylococcus aureus(n= 268, 47%) and viridans group streptococci (n= 127, 22%). The most common manifestations were vegetation in the mitral (n = 222, 36%), aortic (n = 214, 34%), and tricuspid valves (n = 117, 19%). Correlations were seen between aortic valve vegetation and coagulase-negative staphylococci (CoNS) and Enterococcus faecalis, between mitral valve vegetation and group B streptococci, tricuspid valve vegetation, andS.aureus, and between perivalvular abscesses and CoNS (all P< 0.05).
Conclusions
Correlations were found between certain bacteria and specific ECHO manifestations. Our study contributes to a better understanding of IE manifestations and their underlying bacterial etiology, which pathogens can cause severe infections and might require close follow-up and surgical treatment.
Robotic radical antegrade modular pancreatosplenectomy(RAMPS) versus standard retrograde pancreatosplenectomy (SRPS):study protocol for a randomized controlled trial
Authors: Zhang Gong , Liu Rong , Kang Yuhao , Zhang Haifeng , Wang Fei 
Published: August 2019

Abstract
Background There has been data from meta-analysis suggesting that RAMPS is a safe and effective procedure for adenocarcinoma in the body or tail of the pancreas and is oncologically superior to SRPS. However, previous studies on RAMPS were conducted under the open and laparoscopic surgery. Robotic surgery, on the other hand, plays a role in ergonomics and offers several advantages, including less fatigue, tremor filtering, 7° of wrist-like motion, motion scaling, and three-dimensional vision. At present, there is still a lack of clinical studies to observe the safety and clinical efficacy of Robotic RAMPS in the world. Hence, prospective randomized controlled trials (RCTs) comparing Robotic RAMPS and SRPS are required. We begin a RCT trial to compare short-term surgical and oncological outcomes of Robotic RAMPS and SRPS for patients with distal pancreatectomy.Methods This is a randomized, single-center clinical trial. All included adults are patients with primary carcinoma of the distal pancreatectomy. A total of 246 patients will be randomly allocated to Robotic RAMPS or SRPS. The primary endpoints are oncological outcomes(R0 rate, number of Lymph node). Secondary endpoints are the perioperative complications, Perioperative indicators(operative time, blood loss, blood transfusion volume, costs).Discussion To evaluate the surgical and oncological outcomes of Robotic RAMPS, we therefore undertake a prospective RCT. This procedure may become a standard approach to robotic pancreatosplenectomy.
Antibiotic use among twelve Canadian First Nations communities: A retrospective chart review of skin and soft tissue infections
Authors: Jeong Dahn , Nguyen Ha Nhan Thi , Tyndall Mark , Schreiber Yoko S 
Published: August 2019

Abstract
Background Previous publications indicated an emerging issue with community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA), particularly skin and soft tissue infections (SSTIs), in Indigenous communities in Canada. The objectives of this analysis were to explore the prevalence of SSTIs due to CA-MRSA and patterns of antimicrobial use in the community setting.Methods A retrospective chart review was conducted as part of an environmental scan to assess antibiotic prescriptions in 12 First Nations communities across five provinces in Canada including Alberta, Saskatchewan, Manitoba, Ontario, and Québec. Charts were randomly selected from nursing stations and patients who had accessed care in the previous 12 months and were ≥18 years were included in the review. Data was collected from September to December, 2013 on antibiotic prescriptions, including SSTIs, clinical symptoms, diagnostic information including presence of CA-MRSA infection, and treatment.Results A total of 372 charts were reviewed, 60 from Alberta, 70 from Saskatchewan, 120 from Manitoba, 100 from Ontario, and 22 from Québec. Among 372 patients, 224 (60.2%) patients had at least one antibiotic prescription in the previous 12 months and 569 prescriptions were written in total. The prevalence of SSTIs was estimated at 36.8% (137 cases of SSTIs in 372 charts reviewed). In 137 cases of SSTIs, 34 (24.8%) were purulent infections, and 55 (40.2%) were due to CA-MRSA.Conclusions This study has identified a high prevalence of antibiotic use and SSTIs due to CA-MRSA in remote and isolated Indigenous communities across Canada. This population is currently hard to reach and under-represented in standard surveillance system and randomized retrospective chart reviews can offer complimentary methodology for monitoring disease burden, treatment and prevention.
Sleeping in cooking room is associated with under-five pneumonia in North West Ethiopia: prospective cross-sectional study
Authors: Alemu Abebaw Yeshambel , Azezew Amlaku , Alebachew Wubet , Minuye Biniam 
Published: August 2019

Abstract
Background Pneumonia is the one of the five-leading cause under-five morbidity and mortality in developing countries. It is the leading cause of under-five death in Ethiopia. However, data on its institutional burden and factors associated was not explored enough in the study area. Therefore, this study was aimed to asses institution-based proportion of under-five pneumonia and associated factors at Debre Tabor General Hospital.Methods Institution based cross sectional survey was conducted from January 3 to February 10/2019 on a total of 342 participants. Data was collected using structured questionnaire. Additionally, medical charts were reviewed for objective diagnosis of pneumonia. After entered by EPI info version 7 data was exported to SPSS version 20 for further analysis. The result was summarized and presented using descriptive statistics. Logistic regression was computed to assess statistical association at 95% confidence level and p-value ≤0.05.Result Among all under-five children visiting under-five outpatient department 27.5% were diagnosed for pneumonia. Participants who were not sleeping in cooking room; 0.066 (0.035,0.123)), babies who had malnutrition (AOR (95% CI); 7.897 (2.480,25.145)), no breast feeding (AOR (95% CI); 5.805 (1.477,22.821)), and mixed breast feeding (AOR (95% CI); 4.111 (1.152,14.672)) were significantly associated with increased proportion of under-five pneumonia among children visiting Debre Tabor General Hospital under-five outpatient department.Conclusion Institution-based proportion of under-five pneumonia was high in this study. Sleeping in cooking room, breast feeding and malnutrition were associated with under-five pneumonia. Therefore, health education on exclusive breastfeeding, separate cooking and sleeping room as well as improve nutrition status of children could reduce proportion of under-five pneumonia.
Loop ileostomy closure: a retrospective comparison of three techniques
Authors: Lord Ian , Reeves Lesley , Gray Andrew , Woodfield John , Clifford Kari , Thompson-Fawcett Mark 
Published: August 2019

Abstract
Background Loop ileostomy (LI) formation is a common practice for patients undergoing low anterior resection or restorative ileo-anal pouch surgery. This can be performed using a stapled or hand-sewn technique, with or without resection. If hand-sewn, the closure can be one or two layers. Randomised controlled trials have not demonstrated one technique to be superior, and meta-analyses are limited by the heterogeneity of published studies. Our primary aim is to compare stapled ileostomy closure with single- and two-layer, hand-sewn closures.Methods This retrospective, single-centre cohort study included patients undergoing LI closure between January 1999 – April 2016. Patient demographics, anastomotic technique, operative time, and patient outcomes were collected.Results Our analysis included 244 patients (median age 67 years, 43.4% female). There were no significant differences in mean operative times (71.5, 73.1, and 88.5 minutes, for stapled, single-, and two-layer hand-sewn closures respectively, adjusted overall p=0.262), or morbidity (21.5% vs 20.4% vs 17.6%, adjusted overall p=0.934) between stapled or hand-sewn anastomoses, and no mortality. Once adjusting for age, sex, ASA, and consultant surgeon, the LOS was similar between stapled and single-layer closures (4.2 vs 5.5 days, p=0.105), but significantly different between stapled and two-layer closures (4.2 vs 8.3 days, p=0.026) (overall p=0.034). No evidence of differences in complications was found.Conclusions Stapled and single-layered hand-sewn closures are similar in length of procedure, LOS, and complication rates. A two-layer, hand-sewn technique is associated with a significant increase in stay compared to a stapled ileostomy closure.