bluuebunny/crossref_metadata_2025_split huggingface dataset:
Friction and Wear of MoO3/Graphene Oxide Modified Glass Fiber Reinforced Epoxy Nanocomposites
Authors: He Yuxin , Chen Qiuyu , Liu Hu , Zhang Li , Wu Dongyang , Lu Chang , OuYang Wei , Jiang Danfeng , Wu Mingfang , Zhang Jiaoxia , Li Yingchun , Fan Jincheng , Liu Chuntai , Guo Zhanhu 
Published: June 2019

AbstractIn order to further improve the tribological performance of glass fiber reinforced epoxy (GF/EP) composites, highly flexible, binder‐free, molybdenum trioxide MoO3 nanobelt/graphene oxide (GO) film (f‐MoO3‐GO) is prepared by a hydrothermal method. Herein, f‐MoO3‐GO is adopted to modify GF/EP composites prepared through the vacuum‐assisted resin transfer molding method. The neat GF/EP and MoO3‐GO modified GF/EP composites are also fabricated for comparison. The tribological performance is performed using a ball‐on‐disc (“steel‐on‐polymer”) configuration under a dry sliding condition. The coefficient of friction is reduced from 0.61 for neat GF/EP composites down to 0.23 for f‐MoO3‐GO modified GF/EP (f‐MoO3‐GO/GF/EP) composites and the anti‐wear performance is improved by more than four times. The worn surface morphological observation for the composite samples is used to explain the possible wear micro‐mechanisms. The wear reducing effect of the f‐MoO3‐GO/GF/EP composites can be assigned to the increased self‐lubricating effect of f‐MoO3‐GO. With the combined advantageous properties of the used individual components, these unique composites can be used for many other applications.
CDC25B and CDC25C overexpression in nonmelanoma skin cancer suppresses cell death
Authors: Al‐Matouq Jenan , Holmes Thomas R. , Hansen Laura A. 
Published: June 2019

AbstractNon‐melanoma skin cancer frequently results from chronic exposure to ultraviolet (UV) irradiation. UV‐induced DNA damage activates cell cycle arrest checkpoints through degradation of the cyclin‐dependent kinase activators, the cell division cycle 25 (CDC25) phosphatases. We previously reported increased CDC25A in nonmelanoma skin cancer, but CDC25B and CDC25C had not been previously examined. Consequently, we hypothesized that increased expression of CDC25B and CDC25C increases tumor cell proliferation and skin tumor growth. We found that CDC25B and CDC25C were increased in mouse and human skin cancers. CDC25B was primarily cytoplasmic in skin and skin tumors and was significantly increased in the squamous cell carcinoma (SCC), while CDC25C was mostly nuclear in the skin, with an increased cytoplasmic signal in the premalignant and malignant tumors. Surprisingly, forced expression of CDC25B or CDC25C in cultured SCC cells did not affect proliferation, but instead suppressed apoptosis, while CDC25C silencing increased apoptosis without impacting proliferation. Targeting CDC25C to the nucleus via mutation of its nuclear export sequence, however, increased proliferation in SCC cells. Overexpression of CDC25C in the nuclear compartment did not hinder the ability of CDC25C to suppress apoptosis, neither did mutation of sites necessary for its interaction with 14‐3‐3 proteins. Analysis of apoptotic signaling pathways revealed that CDC25C increased activating phosphorylation of Akt on Ser473, increased inhibitory phosphorylation of proapoptotic BAD on Ser136, and increased the survival protein Survivin. Silencing of CDC25C significantly reduced Survivin levels. Taken together, these data suggest that increased expression of CDC25B or CDC25C are mechanisms by which skin cancers evade apoptotic cell death.
Walnut‐Derived Peptide PW5 Ameliorates Cognitive Impairments and Alters Gut Microbiota in APP/PS1 Transgenic Mice
Authors: Wang Min , Amakye William Kwame , Guo Lu , Gong Congcong , Zhao Yuanhui , Yao Maojin , Ren Jiaoyan 
Published: June 2019

ScopeDecreasing β‐amyloid (Aβ) accumulation is of significance in finding therapeutic candidates for cognitive impairments in Alzheimer's disease (AD). The aim of this study is to investigate the potency of the active components of walnut protein in decreasing Aβ aggregation and ameliorating cognitive impairments.Methods and resultsCell model of intracellular Aβ42 aggregation is used to explore the active ingredients in walnut protein hydrolysate (WPH). A bioactive peptide (Pro‐Pro‐Lys‐Asn‐Trp, PW5) with great anti‐Aβ42 aggregation activity identified from the WPH is synthesized for in vitro and in vivo experiments. Using classic APP/PS1 mouse model, it is validated that PW5 exerts its effects on cognitive improvement through reducing Aβ plaques accumulation. Moreover, metabolomic analysis reveals that serum norepinephrine (NE) and isovalerate levels are significantly increased in response to PW5 intervention, with decreased serum levels of acetylcholine (AChe) and valerate, compared with the vehicle‐treated APP/PS1 mice. PW5 feeding also improves gut dysbiosis in APP/PS1 transgenic mice by increasing the relative abundance of Firmicutes and decreasing Proteobacteria and Verrucomicrobia as displayed by 16s rRNA analyses.ConclusionsThese promising results support the utilization of peptide PW5 as an active ingredient in functional foods or potential drug candidate for the prevention and/or treatment of AD.
Integrative genomic analysis of matched primary and metastatic pediatric osteosarcoma
Authors: Negri Gian Luca , Grande Bruno M , Delaidelli Alberto , El‐Naggar Amal , Cochrane Dawn , Lau Ching C , Triche Timothy J , Moore Richard A , Jones Steven JM , Montpetit Alexandre , Marra Marco A , Malkin David , Morin Ryan D , Sorensen Poul H 
Published: June 2019

AbstractDespite being the most common childhood bone tumor, the genomic characterization of osteosarcoma remains incomplete. In particular, very few osteosarcoma metastases have been sequenced to date, critical to better understand mechanisms of progression and evolution in this tumor. We performed an integrated whole genome and exome sequencing analysis of paired primary and metastatic pediatric osteosarcoma specimens to identify recurrent genomic alterations. Sequencing of 13 osteosarcoma patients including 13 primary, 10 metastatic, and 3 locally recurring tumors revealed a highly heterogeneous mutational landscape, including cases of hypermutation and microsatellite instability positivity, but with virtually no recurrent alterations except for mutations involving the tumor suppressor genes RB1 and TP53. At the germline level, we detected alterations in multiple cancer related genes in the majority of the cohort, including those potentially disrupting DNA damage response pathways. Metastases retained only a minimal number of short variants from their corresponding primary tumors, while copy number alterations showed higher conservation. One recurrently amplified gene, KDR, was highly expressed in advanced cases and associated with poor prognosis. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Pilot study for the fidelity, acceptability, and effectiveness of a PBIS program plus mental health supports in under‐resourced urban schools
Authors: Eiraldi Ricardo , McCurdy Barry , Schwartz Billie , Wolk Courtney Benjamin , Abraham Manju , Jawad Abbas F. , Nastasi Bonnie K. , Mautone Jennifer A. 
Published: June 2019

AbstractThis paper describes implementation (fidelity, perceived acceptability) and tier 1 and Tier 2 outcomes of school‐wide positive behavior interventions and supports approach including mental health supports at Tier 2 in two K‐8 urban schools. Interventions for Tier 2 consisted of three manualized group cognitive behavioral therapy (GCBT) protocols for externalizing behavior problems, depression, and anxiety. Tier 1 and Tier 2 interventions were implemented with fidelity but program feasibility for Tier 2 was in question because school personnel needed a great deal of external support to implement the interventions. Tier 1 interventions were associated with a decrease in office discipline referrals. Students participating in GCBT showed a significant decrease in mental health diagnostic severity at posttreatment. A discussion of perceived and actual implementation barriers and how they were addressed is provided. Implications for practice in low‐income urban schools are discussed.
Assessing psychosocial risk in pediatric cystic fibrosis
Authors: Filigno Stephanie S. , Miller Jamie , Moore Susan , Peugh James , Weiland Jeanne , Backstrom Jessica , Borschuk Adrienne 
Published: June 2019

AbstractBackgroundPsychosocial risk factors are known to impact quality of life, treatment adherence, and health outcomes. No standardized comprehensive psychosocial risk screener is routinely utilized in cystic fibrosis (CF) care. The objectives of the study were to describe the range and severity of psychosocial risk within this CF population, investigate the reliability of a comprehensive psychosocial screener in pediatric CF clinical care, and explore relationships between psychosocial risk and key factors affecting health outcomes. It was hypothesized that the PAT‐CF total and subscale α coefficients would be similar to those found in other pediatric medical populations.MethodParents of 154 children with CF completed a CF‐specific version of the Psychosocial Assessment Tool_All‐lit (PAT‐CF), an empirically‐based psychosocial risk assessment, during routine CF clinical care.ResultsThe internal consistency of the PAT‐CF Total score was 0.71. Total score and subscale reliabilities reflect findings in other pediatric populations. Total risk scores fell in the following categories: 7% (Clinical‐highest risk), 41% (Targeted), and 52% (Universal‐lowest risk), respectively. Increased psychosocial risk was associated with Medicaid status and lower parent education, whereas having private insurance was associated with decreased psychosocial risk.ConclusionsThe PAT‐CF can feasibly be used as an empirically‐based comprehensive psychosocial risk tool in routine CF care and is acceptable by parents. In addition to providing universal anticipatory guidance regarding child and family wellness, early identification of risk factors allows care teams to proactively provide targeted support and intervention for specific psychosocial risk factors to promote improved quality of life and ability to sustain daily care.
Effect of alcohol on chronic pelvic pain and prostatic inflammation in a mouse model of experimental autoimmune prostatitis
Authors: Zhang Li‐Gang , Chen Jing , Meng Jia‐Lin , Zhang Yong , Liu Yi , Zhan Chang‐Sheng , Chen Xian‐Guo , Zhang Li , Liang Chao‐Zhao 
Published: June 2019

AbstractBackgroundChronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a prevalent disease of the urogenital system. Alcohol has been reported to be closely related to CP/CPPS. Thus, we intended to verify the role of alcohol in CP/CPPS and determine the underlying mechanism.MethodsWe induced experimental autoimmune prostatitis (EAP) mouse model by intradermally injecting a mixture of prostate antigens (PAgs) and complete Freund's adjuvant on days 0 and 28. Mice were treated with alcohol (control‐alcohol and EAP‐alcohol groups) or vehicle (control‐vehicle, and EAP‐vehicle groups) from day 32 to 42. Forty‐two days after PAg injection, the pathological appearance of the prostate tissues was evaluated, and histological analyses of the prostate were performed. Chronic pelvic pain was assessed by applying von Frey filaments to the lower abdomen. Proinflammatory cytokines were detected by enzyme‐linked immunosorbent assay tests. Then, we explored the effects of the NLRP3 inhibitor MCC950 on chronic pelvic pain and prostatic inflammation in this model.ResultsHistological analyses showed diffuse inflammation in the stromal tissues that were characterized by severe infiltration of neutrophils and mononuclear cells in mice in the EAP‐alcohol group compared with EAP‐vehicle group. Chronic pain tests showed that the response frequency was significantly increased using a von Frey filament at forces of 0.4, 1.0, and 4.0 g in EAP‐alcohol group compared with EAP‐vehicle (P < .05). The levels of proinflammatory cytokines, including interferon (IFN)‐γ, tumor necrosis factor (TNF)‐α, IL‐17, and IL‐1β were all significantly elevated in EAP‐alcohol group compared with the EAP‐vehicle group (P < .05). However, between the control‐alcohol and control‐vehicle groups, chronic pain tests, histological assays, and cytokine determinations showed no differences. Furthermore, our results demonstrated that MCC950 could decrease the expression level of NLRP3 inflammasome–related proteins including NLRP3, ASC, and caspase‐1. The chronic pain tests, histological assays, and cytokine determinations showed that MCC950 could attenuate the chronic pain and prostatic inflammation through the inhibition of the NLRP3 inflammasome.ConclusionsThis study indicated that alcohol could aggravate the severity of prostatic inflammation in EAP model though activating the NLRP3 inflammasome. Furthermore, the role of MCC950 in inhibiting NLRP3 inflammasome and decreasing IL‐1β secretion to alleviate EAP severity may show that it is a promising therapeutic agent for CP/CPPS.
Concentration‐dependent effects of dutasteride on prostate‐specific membrane antigen (PSMA) expression and uptake of 177Lu‐PSMA‐617 in LNCaP cells
Authors: Kranzbühler Benedikt , Salemi Souzan , Umbricht Christoph A. , Deberle Luisa M. , Müller Cristina , Burger Irene A. , Hermanns Thomas , Sulser Tullio , Eberli Daniel 
Published: June 2019

AbstractBackgroundProstate‐specific membrane antigen (PSMA)‐based imaging and therapy are increasingly used in the management of prostate cancer. However, low PSMA surface expression in certain patients is a limitation for PSMA‐based technologies. We have previously shown that high doses of dutasteride, a 5α‐reductase inhibitor generally used for the treatment of benign prostatic enlargement, increase the PSMA expression in vitro. We now further analyzed the concentration‐ and time‐dependent effects of dutasteride in LNCaP cells.MethodsAndrogen receptor (AR) expressing prostate cancer cells (LNCaP) were treated for 7 to 14 days with vehicle control (0.1% dimethyl sulfoxide) or different concentrations of dutasteride (0.25 , 0.5 , 1 , and 5  μM). In addition to cell proliferation, PSMA surface expression was assessed using flow cytometry (FACS) and immunocytochemistry. Total PSMA and AR expression was analyzed by capillary western immunoassay (WES). In addition, tumor cell uptake and internalization assays of 177Lu‐PSMA‐617 were performed.ResultsDutasteride treatment resulted in a significant upregulation of PSMA surface expression compared to vehicle control after 7 days in all tested concentrations. After 14 days a further, concentration‐dependent increase of PSMA surface expression was detectable. Total PSMA protein expression significantly increased after treatment of cells with high concentrations of dutasteride using 5  μM for 7 or 14 days. However, when lower concentrations were used total PSMA expression was not significantly altered compared to vehicle control. Further testing revealed a dose‐dependent increase in uptake and internalization of 177Lu‐PSMA‐617 after 7 and 14 days. Though, a significantly increased uptake was only observed using a 5  μM dutasteride concentration for 7 days as well as 1  and 5  μM for 14 days.ConclusionOur investigations revealed a concentration‐ and time‐dependent effect of dutasteride on PSMA expression and uptake of 177Lu‐PSMA‐617 in LNCaP cells. A short‐term treatment of patients with high doses of dutasteride might increase the detection rate of PSMA‐based imaging and increase the effect of 177Lu‐PSMA‐617 therapy via upregulation of PSMA expression.
Improved CUSUM monitoring of Markov counting process with frequent zeros
Authors: Kim Hanwool , Lee Sangyeol 
Published: June 2019

AbstractThere has been a growing interest in monitoring processes featuring serial dependence and zero inflation. The phenomenon of excessive zeros often occurs in count time series because of the advancement of quality in manufacturing process. In this study, we propose three control charts, such as the cumulative sum chart with delay rule (CUSUM‐DR), conforming run length (CRL)‐CUSUM chart, and combined Shewhart CRL‐CUSUM chart, to enhance the performance of monitoring Markov counting processes with excessive zeros. Numerical experiments are conducted based on integer‐valued autoregressive time series models, for example, zero‐inflated Poisson INAR and INARCH, to evaluate the performance of the proposed charts designed for the detection of mean increase. A real example is also illustrated to demonstrate the usability of our proposed charts.
Investigation into the molecular structure and energetic stability of endohedral and exohedral metallofullerene derivatives of C24
Authors: Haynes Caleb A. , Lopez Serafin , Beran Kyle A. 
Published: June 2019

AbstractDensity‐functional theory was applied to the investigation of the structural and electronic properties of C24 fullerene derivatives. Transition metals (TMs) from groups 11 and 12, in various oxidation and spin‐states, are inserted at either endohedral (TM@C24) or exohedral (TM‐C24) sites and their subsequent energetic stabilities are assessed. With the exception of Ag@C24, all derivatives are predicted to occupy a minimum on the potential energy surface. The optimized exohedral TM‐C24 geometries yield TM‐C bond lengths that are consistent with comparable carbon‐metal bond lengths, and the overwhelming majority of the derivatives result in a slight deformation of the C24 cage as the bonding carbon takes on more sp3 character. All of the TM@C24 equilibrium structures maintain the integrity of the cage structure with a moderate increase in the diameter. All neutral exohedral and endohedral complexes favor the low spin‐state; conversely, all of the charged exohedral complexes prefer the high spin‐state, with the exception of Cu‐C241+ molecular ion. The Group 12 charged endohedral derivatives prefer the low spin‐state, whereas the Group 11 molecular ions do not necessarily exhibit a definitive trend. Analysis of the energetic data predicts that of the lowest energy endohedral molecular species only four are predicted to be energetically favorable in terms of insertion energy and an advantageous HOMO‐LUMO gap: Cu@C242+, Ag@C241+, Au@C243+, and Zn@C242+.
Formation of Single Micro‐ and Nanowires with Extreme Aspect Ratios in Microfluidic Channels
Authors: Lenz Mario , Sebastian Bernhard , Dittrich Petra S. 
Published: June 2019

AbstractShown here is the site‐specific formation of single extraordinarily long metal–organic micro‐ and nanowires using a microfluidic device made of poly(dimethylsiloxane) (PDMS). This approach exploits two concepts, i) the diffusion of organic precursor molecules through PDMS and ii) the use of microfluidic channels as a growth template. To initiate wire formation, metal and organic precursor solutions are filled into different supply channels that are separated by PDMS. As the precursor diffuses through PDMS, and thereby infiltrates the adjacent channel, the growth of micro‐ and nanowires starts at the side walls of this adjacent channel. The formation yields single wires with sizes ranging from several hundreds of micrometers to millimeters at diameters of 0.5–2 µm. The principles of this formation pathway are demonstrated with the reaction of tetrathiafulvalene (TTF) and gold(III) ions that yields Au‐TTF wires. The influence of various reaction parameters including the choice of solvents and the chip fabrication protocol on the reaction are evaluated. Based on these findings, a further microfluidic device design with orthogonally arranged channels is developed, and the formation of single wires in a channel‐defined pattern is demonstrated. Moreover, the possibility of pulsed precursor supply allows for advanced control over the growth of the wires.
Design of Hierarchical Beads for Efficient Label‐Free Cell Capture
Authors: Sun Shiyu , Wang Ruimin , Huang Yida , Xu Jiale , Yao Kuan , Liu Wanshan , Cao Yimei , Qian Kun 
Published: June 2019

AbstractDefined hierarchical materials promise cell analysis and call for application‐driven design in practical use. The further issue is to develop advanced materials and devices for efficient label‐free cell capture with minimum instrumentation. Herein, the design of hierarchical beads is reported for efficient label‐free cell capture. Silica nanoparticles (size of ≈15 nm) are coated onto silica spheres (size of ≈200 nm) to achieve nanoscale surface roughness, and then the rough silica spheres are combined with microbeads (≈150–1000 µm in diameter) to assemble hierarchical structures. These hierarchical beads are built via electrostatic interaction, covalent bonding, and nanoparticle adherence. Further, after functionalization by hyaluronic acid (HA), the hierarchical beads display desirable surface hydrophilicity, biocompatibility, and chemical/structural stability. Due to the controlled surface topology and chemistry, HA‐functionalized hierarchical beads afford high cell capture efficiency up to 98.7% in a facile label‐free manner. This work guides the development of label‐free cell capture techniques and contributes to the construction of smart interfaces in bio‐systems.
Confined Pyrolysis of ZIF‐8 Polyhedrons Wrapped with Graphene Oxide Nanosheets to Prepare 3D Porous Carbon Heterostructures
Authors: Ding Bing , Fan Zengjie , Lin Qingyang , Wang Jie , Chang Zhi , Li Tao , Henzie Joel , Kim Jeonghun , Dou Hui , Zhang Xiaogang , Yamauchi Yusuke 
Published: June 2019

AbstractHeterostructured materials are interesting because they may combine two or more material building blocks that together generate new types of heterointerfaces with unusual properties. Using them to construct large‐scale 3D frameworks further extends their utility in electrochemical applications because it exposes more interfaces and active sites. In this study, electrostatic interactions are used to wrap polyhedra particles of zeolitic imidazolate frameworks with graphene oxide (GO) nanosheets to prepare the composite structure. Pyrolyzing this structure generates a 3D porous carbon framework (PCF) composed of polyhedral‐shaped hollow carbon coated with reduced GO. The size of the polyhedral macropores can be adjusted from nanometer scale to micrometer scale. The PCFs generate a continuous network of heterostructured carbon with a large surface area and large pore volumes that are particularly useful as porous electrodes in lithium–sulfur batteries. The PCF/S composite electrode exhibits a high discharge capacity of 1151 mAh g−1 at 1 C and a low capacity decay of 0.035% per cycle after 650 cycles.
Mechanical and fracture properties of recycled aggregate concrete in design codes and empirical models
Authors: Ghorbel Elhem , Wardeh George , Fares Hanaa 
Published: June 2019

AbstractIn this paper the relationships between the mechanical and fracture properties of recycled aggregate concretes (RAC) are investigated based on the own research programs results and an extensive number of experimental data from the literature. The primary aim is the extension of design standards empirical models to the case of RAC with particular emphasis on the effect of replacement ratio on mechanical properties, uniaxial stress–strain curve, fracture energy, and stress–strain softening curve's parameters.It was pointed out that relationships dedicated to assess the mechanical properties of natural aggregate concretes (NAC) are not fully adequate to predict the behavior of RAC. It was established that the elastic modulus Ecm, the tensile splitting strength fctm,sp are related to the mean compressive strength fcm as well as to a parameter taking into account the effect of the recycled aggregates replacement ratio. The validity of many analytical expressions of the stress–strain relationship has been also studied and the effect of replacement ratio was taken into account. The comparison between experimental and analytical stress–strain curves shows that, with the proposed modifications, some models satisfactorily describe the behavior up to failure while others either overestimate or underestimate the postpeak branch. Due to the limited number of available data, no well‐defined relation was found between fracture energy, compressive strength, and formulation parameters.