bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the relationship between psychoacoustic and affective variables in a shutter‐press sound
Authors: Rhie Ye Lim , Kim Ga Won , Yun Myung Hwan 
Published: February 2019

AbstractWhereas smart products have emerged as a new product category, almost no studies have introduced product sounds that give a smart impression. Moreover, technological development has increased the need to shape or design consequential sounds, which have been recognized to depend on the mechanical properties of a product. Therefore, we introduce an approach to design consequential sounds for a camera‐shutter sound. First, we elicit design variables from existing camera‐shutter sounds and conduct an experiment to examine their effects on smartness and satisfaction. Next, the time structure‐related variables of a camera‐shutter sound are modified and evaluated with eight sound descriptors. The results demonstrate the relationship between design variables and affective variables by adopting the concept of psychoacoustic variables. The proposed method might be able to assist in designing a consequential sound to present both smart impressions and other targeted sound impressions depending on the research purpose.
Pelvic floor myofascial pain in patients with symptoms of urinary tract infection
Authors: Wolff Birte J. , Joyce Cara J. , Brincat Cynthia A. , Mueller Elizabeth R. , Fitzgerald Colleen M. 
Published: February 2019

AbstractObjectiveTo correlate lower urinary tract symptoms typically associated with a urinary tract infection (UTI) with physical examination findings of pelvic floor myofascial pain (PFMP).MethodsThis retrospective review included all new patients presenting to a urogynecology clinic between August 2 and December 19, 2016. Patients completed validated questionnaires, had a catheterized urine specimen, and underwent pelvic examination. Associations between demographics, symptoms, urine culture, and PFMP were analyzed.ResultsWe included 250 patients with urinary frequency (n=160, 64.0%), urgency (n=155, 62.0%), urgency incontinence (n=140, 56.0%), pelvic pain (n=43, 17.2%), and dysuria (n=25, 10.0%). PFMP was detected in 125 (50.0%) patients and culture‐proven UTI in 15 (6.0%) patients. Demographics associated with PFMP were lower prolapse stage (P<0.001), age younger than 50 years (P<0.001), lower parity (P=0.028), and non‐white ethnicity (P=0.003). Symptoms associated with PFMP were dysuria (adjusted odds ratio 4.13, 95% confidence interval 1.08–15.78), urgency/frequency (2.72, 1.47–5.04), and patient‐reported pelvic pain (2.57, 1.08–6.12). These symptoms were independent predictors in multivariable logistic regression analysis.ConclusionsMost patients had symptoms associated with UTI; however, culture‐confirmed diagnosis was infrequent and PFMT was diagnosed in half of participants. Clinicians treating women with these symptoms are advised to examine the pelvic floor muscles.
Renal transplant anastomotic pseudoaneurysms: Case report of open repair and endovascular management
Authors: Sharma Nitin , Bidnur Samir , Caldas Mauricio , McNally Dermot , Murray Allan , Turnbull Robert , Todd Gerald T , Moore Ronald B 
Published: February 2019

IntroductionAnastomotic pseudoaneurysm is one of the rarest vascular complications after renal transplant surgery. Therapeutic options include open surgical repair or endovascular stenting.Case presentationCase 1 had pseudoaneurysm involving external iliac artery and was managed by jump graft to allograft using cadaveric donor iliac arteries and patch angioplasty repair of external iliac artery after excising pseudoaneurysm. Case 2 had undergone orthotopic renal transplant with spleno‐renal arterial anastomosis and developed a massive pseudoaneurysm proximal to spleno‐renal arterial anastomosis. This patient underwent endovascular stenting preserving allograft vascularity and graft function. Outcome in both patients was successful with normalization of renal function to baseline levels.ConclusionTreatment of renal transplant anastomotic pseudoaneurysms is difficult and associated with high rates of graft loss. Open surgery is the gold standard providing several possibilities for arterial reconstruction preserving graft and limb circulation. Endovascular treatment should be considered in high‐risk surgical patients with favorable anatomy.
The Emerging Role of Osteocytes in Cancer in Bone
Authors: Atkinson Emily G , Delgado‐Calle Jesús 
Published: February 2019

ABSTRACTAdvances in the last decade have established the osteocyte, the most abundant cell in bone, as a dynamic and multifunctional cell capable of controlling bone homeostasis by regulating the function of both osteoblasts and osteoclasts. In addition, accumulating evidence demonstrates that osteocyte function is altered in several skeletal disorders, and targeting osteocytes and their derived factors improves skeletal health. Despite the remarkable progress in our understanding of osteocyte biology, there has been a paucity of information regarding the role of osteocytes in the progression of cancer in bone. Exciting, recent discoveries suggest that tumor cells communicate with osteocytes to generate a microenvironment that supports the growth and survival of cancer cells and stimulates bone destruction. This review features these novel findings and discussions regarding the impact of chemotherapy on osteocyte function and the potential of targeting osteocytes for the treatment of cancer in bone. © 2019 The Authors.JBMR Pluspublished by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.
Therapeutic effect of double plasma molecular adsorption system and sequential half‐dose plasma exchange in patients with HBV‐related acute‐on‐chronic liver failure
Authors: Yao Jia , Li Shuang , Zhou Li , Luo Lei , Yuan Lili , Duan Zhongping , Xu Jun , Chen Yu 
Published: February 2019

AbstractObjectiveThe artificial liver support system (ALSS) is used frequently as a first‐line treatment for hepatitis B virus‐associated acute‐on‐chronic liver failure (HBV‐ACLF). This study aims to compare the therapeutic efficacy of double plasma molecular adsorption system (DPMAS) with sequential half‐dose plasma exchange (PE) (DPMAS+PE) and full‐dose PE in patients with HBV‐ACLF.MethodsA total of 131 hospitalized patients who were diagnosed with HBV‐ACLF and underwent DPMAS+PE or PE were retrospectively analyzed. According to the treatment methods used, they were divided into PE group (n = 77) and DPMAS+PE group (n = 54). The main evaluation indexes included the change of liver function and the 28‐days liver transplant‐free survival rates after the different treatments.ResultsThere were no significant differences on severity of illness between PE group and DPMAS+PE group (P > 0.05). The total bilirubin (TBIL) levels immediately after treatment, and at 24 and 72 hours after treatment were markedly decreased in DPMAS+PE group than that in PE group (52.3 ± 9.4% vs 42.3 ± 7.2%, P < 0.05; 24.2 ± 10.0% vs 13.5 ± 13.0%, P < 0.05; 24.8 ± 13.1% vs 14.9 ± 14.9%, P < 0.05; respectively). The 28‐days survival rates was 62.3% and 72.2% in PE and DPMAS+PE groups (P = 0.146). Furthermore, the 28‐days survival rates were significantly higher in DPMAS+PE group than that in PE group (57.4% vs 41.7%, P = 0.043) in the intermediate‐advanced stage patients.ConclusionCompared with PE alone, DPMAS+PE might more effectively improve temporary TBIL in ACLF patients, and improve the 28‐days survival rates in HBV‐ACLF patients with intermediate‐advanced stage. Therefore, DPMAS+PE may be an available ALSS treatment for HBV‐ACLF patients.
PKM2‐regulated STAT3 promotes esophageal squamous cell carcinoma progression via TGF‐β1‐induced EMT
Authors: Ma Rong , Liu Qing , Zheng Shutao , Liu Tao , Tan Doudou , Lu Xiaomei 
Published: February 2019

AbstractRecent studies have demonstrated pleiotropic roles of pyruvate kinase isoenzyme type M2 (PKM2) in tumor progression. However, the precise mechanisms underlying the effects of PKM2 on esophageal squamous cell carcinoma (ESCC) metastasis and transforming growth factor β1 (TGF‐β1)‐induced epithelial‐mesenchymal transition (EMT) remain to be established. In this study, we observed upregulation of PKM2 in ESCC tissues that was markedly associated with lymph node metastasis and poor prognosis. High PKM2 expression in tumor tissues frequently coincided with the high pSTAT3Tyr705 expression and low E‐cadherin expression. Furthermore, altered PKM2 expression was significantly associated with proliferation, migration, and invasion of ESCC cells, in addition to expression patterns of EMT markers (Snail, E‐cadherin, and vimentin) and pSTAT3Tyr705/STAT3 ratio. Overexpression of STAT3 significantly attenuated the effects of PKM2 knockdown on cell proliferation and motility as well as expression of pSTAT3
Tyr705 and EMT markers. Consistently, stable short hairpin RNA (shRNA)‐mediated silencing of PKM2 reversed the effects of TGF‐β1 treatment, specifically, upregulation of PKM2, phosphorylation of STAT3 at Tyr705, and increased EMT, migration, and invasion. We propose that PKM2 regulates cell proliferation, migration, and invasion via phosphorylation of STAT3 through TGF‐β1‐induced EMT. Our findings collectively provide mechanistic insights into the tumor‐promoting role of PKM2, supporting its prognostic value and the therapeutic utility of PKM2 inhibitors as potential antitumor agents in ESCC.
Circular RNA expression profiles during the differentiation of human umbilical cord–derived mesenchymal stem cells into cardiomyocyte‐like cells
Authors: Ruan Zhong‐Bao , Chen Ge‐Cai , Zhang Rui , Zhu Li 
Published: February 2019

AbstractSo far, there were no reports on circular RNA (circRNA) expression profiles in the differentiation of human umbilical cord–derived mesenchymal stem cells (hUCMSCs) into cardiomyocyte‐like cells induced by 5‐aza. In this study, hUCMSCs were isolated from umbilical cords and induced with 5‐aza for 14 days. Immunofluorescence staining, real‐time reverse transcription polymerase chain reaction (RT‐PCR), and western blot of cardiac troponin I and α‐sarcomeric actin on hUCMSCs between Days 14 and 0 were performed. The expression profile of circRNAs was analyzed by microarray and validated with RT‐PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses were performed to identify the functions of differentially expressed genes and related pathways. The connections between circRNAs and microRNAs were explored by using Cytoscape. The results showed that a total of 226 circRNAs were calculated as differentially expressed during the differentiation. Among them, 127 were upregulated and 99 were downregulated. We selected circRNAs that were upregulated by more than five‐fold and downregulated by more than three‐fold. Ultimately, 74 differentially expressed circRNAs that were highly conserved on Day 14 after induction compared to Day 0 were identified. Among them, 41 were upregulated and 33 were downregulated. Four upregulated circRNAs (circRNA_01536, circRNA_04411, circRNA_09169, and circRNA_09905) and four downregulated circRNAs (circRNA_00699, circRNA_01183, circRNA_01978, and circRNA_16804) were randomly confirmed by RT‐PCR. GO analysis suggested a number of cell proliferation and differentiation related physiological processes and pathways, such as the Wnt signaling pathway and others. Network analysis uncovered three potential key circRNAs, that is, circRNA_05432, circRNA_08441, and circRNA_01536.
CTGF induces tenogenic differentiation and proliferation of adipose‐derived stromal cells
Authors: Li Xiaoning , Pongkitwitoon Suphannee , Lu Hongbin , Lee Chang , Gelberman Richard , Thomopoulos Stavros 
Published: February 2019

ABSTRACTIntrasynovial tendons are paucicellular and hypovascular, resulting in a poor response to injury. Surgical repair of ruptured or lacerated tendons often lead to complications such as adhesions, repair site gapping, and repair site rupture. Adipose‐derived stem cells (ASCs) have shown promise for enhancing tendon repair, as they have the capacity to differentiate into tendon fibroblasts and augment the healing response. Furthermore, connective tissue growth factor (CTGF) has been shown to promote tendon regeneration via the stimulation of endogenous tendon stem cells. Here, we evaluated the potential of CTGF to promote tenogenic differentiation of ASCs in vitro. Gene and protein expression, cell proliferation, and FAK and ERK1/2 signaling were assessed. CTGF increased tenogenic genes in mouse ASCs in a dose‐ and time‐dependent manner. Western blot and immunostaining analyses demonstrated increases in tenogenic protein expression in CTGF‐treated ASCs at all timepoints studied. CTGF increased ASC proliferation in a dose‐dependent manner. CTGF induced phosphorylation of ERK1/2 within 5 min and FAK within 15 min; both signals persisted for 120 min. Blocking FAK and ERK1/2 pathways by selective inhibitors SCH772984 and PF573228, respectively, attenuated the CTGF‐induced tenogenic differentiation and proliferation of ASCs. These results suggest that CTGF induces tenogenic differentiation of ASCs via the FAK and ERK1/2 pathway. Statement of clinical significance: Although prior research has led to advances in tendon operative techniques and rehabilitation methods, clinical outcomes after tendon repair remain variable, with high rates of repair site gapping or rupture. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res
Effect of protected areas in reducing land development across geographic and climate conditions of a rapidly developing country, Spain
Authors: Rodríguez‐Rodríguez David , Sebastiao Joana , Salvo Tierra Ángel Enrique , Martínez‐Vega Javier 
Published: February 2019

AbstractProtected areas (PAs) aim at safeguarding biodiversity and ecosystem services in the long term. Despite the remarkable growth in area covered by PAs in recent years, biodiversity trends continue to worsen as a result of serious global pressures such as habitat destruction and degradation. One main cause of habitat destruction and degradation is land development that implies the replacement of natural land uses–land covers (LULCs) with artificial ones. Here, we assessed the effectiveness of four PA networks at preventing land development in Spain, a biodiversity‐rich country that has experienced recent rapid environmental transformations, using two models of increased validity: an original model and a biophysically enhanced model. We applied a before–after control–impact (BACI) design whereby absolute artificial area increase (AAI) and relative artificial area increase (RAI) were compared across PA categories (nature reserves [NRs], nature parks [NPs], Sites of Community Importance [SCIs], and Special Protection Areas [SPAs]), study zones (coastal and inland), and climates (Atlantic and Mediterranean) using CORINE Land Cover (CLC) data and two control zones: 1‐ and 5‐km buffers around protected polygons. NRs prevented land development, whereas other categories reduced it moderately to very substantially in the assessed period. AAI was especially intense in inland SPAs and NPs. NRs and NPs were the most effective PA categories inland, whereas NRs and SPAs were the most effective ones on the coast. Land development was greater on the Spanish coast than inland inside and outside PAs, especially around Macaronesian and Mediterranean PAs. Atlantic PAs experienced similar or greater land development values than surrounding areas. Our results are intended to guide future conservation efforts in Spain, chiefly on its heavily pressured coastal environment.
Annual bacterial community cycle in a seasonally ice‐covered river reflects environmental and climatic conditions
Authors: Cruaud Perrine , Vigneron Adrien , Fradette Marie‐Stéphanie , Dorea Caetano C. , Culley Alexander I. , Rodriguez Manuel J. , Charette Steve J. 
Published: February 2019

AbstractBacteria are key players in biogeochemical cycles and control water quality in freshwater ecosystems. Nevertheless, little is known about the identity and ecology of riverine bacteria, especially during ice‐covered periods that are often mistakenly perceived as periods with negligible biological activities. Here, we analyzed in detail the effects of environmental and climatic conditions on freshwater bacterial community structure and diversity over a 2‐yr sampling campaign, targeting a seasonally ice‐covered river of the Quebec City (Canada) area, the Saint‐Charles River. Quantitative polymerase chain reaction and 16S ribosomal RNA gene high‐throughput sequencing demonstrated a strong seasonal cycle of the bacterial community composition with rapid successions of bacterial lineages, reflecting the harsh climatic condition of the region. During the summer, the bacterial community was dominated by typical freshwater microorganisms such as Limnohabitans, Sporichthyaceae hgcI/acI clade, and Pseudarcicella. In contrast, the results suggest that during the cold season, the low water temperatures, combined with other prevailing conditions such as reduced light availability and minimal particulate inputs from the catchment, created various environmental niches for potential methanotrophic Gammaproteobacteria, such as Crenothrix and Methylobacter, other Betaproteobacteria, such as Candidatus Methylopumilus, Candidatus Nitrotoga, and Rhodoferax, as well as Verrucomicrobia and Parcubacteria. The presence of these taxa in the winter suggests active carbon, iron, and nitrogen cycling under ice, whereas summer lineages are dormant or in a phase of reduced activity. These results increase our understanding of bacterial dynamic and potential metabolic processes occurring in seasonally ice‐covered inland waters, providing evidence that winter can be an especially important period for freshwater ecological processes.
Groundwater inflows control patterns and sources of greenhouse gas emissions from streams
Authors: Lupon Anna , Denfeld Blaize A. , Laudon Hjalmar , Leach Jason , Karlsson Jan , Sponseller Ryan A. 
Published: February 2019

AbstractHeadwater streams can be important sources of carbon dioxide (CO2) and methane (CH4) to the atmosphere. However, the influence of groundwater–stream connectivity on the patterns and sources of carbon (C) gas evasion is still poorly understood. We explored these connections in the boreal landscape through a detailed study of a 1.4 km lake outlet stream that is hydrologically fed by multiple topographically driven groundwater input zones. We measured stream and groundwater dissolved organic C (DOC), CO2, and CH4 concentrations every 50 m biweekly during the ice‐free period and estimated in‐stream C gas production through a mass balance model and independent estimates of aquatic metabolism. The spatial pattern of C gas concentrations was consistent over time, with peaks of both CH4 and CO2 concentrations occurring after each groundwater input zone. Moreover, lateral C gas inputs from riparian soils were the major source of CO2 and CH4 to the stream. DOC mineralization and CH4 oxidation within the stream accounted for 17–51% of stream CO2 emissions, and this contribution was the greatest during relatively higher flows. Overall, our results illustrate how the nature and arrangement of groundwater flowpaths can organize patterns of stream C concentrations, transformations, and emissions by acting as a direct source of gases and by supplying organic substrates that fuel aquatic metabolism. Hence, refined assessments of how catchment structure influences the timing and magnitude of groundwater–stream connections are crucial for mechanistically understanding and scaling C evasion rates from headwaters.
Spatial Variability in Size Structure, Growth, and Recruitment of Spotted Seatrout among Six Florida Estuaries
Authors: Herdter Elizabeth , Mahmoudi Behzad , Peebles Ernst , Murawski Steven A. 
Published: February 2019

Abstract
Spotted Seatrout Cynoscion nebulosus in Florida, USA, are managed under four geographical regions, including Apalachicola Bay and Cedar Key (northwest region), Tampa Bay and Charlotte Harbor (southwest region), northeast Florida (northeast region), and the northern Indian River Lagoon (southeast region). Two of these management regions are composed of more than one major estuary system (northwest and southwest regions). However, previous life history research suggests that the management regions do not accurately reflect the unique biological populations of Spotted Seatrout in Florida. Our objective was to determine (1) whether there is significant spatial variation in size structure, the age–length relationship, and recruitment patterns among populations of Spotted Seatrout in Florida; and (2) whether there is agreement in these life history parameters among estuary populations comprising individual management regions. We used long-term fishery-independent and fishery-dependent data sets on recruitment, age, and size structure among six estuaries and the four management regions. Young-of-the-year recruitment patterns and the length-at-age relationship over all ages differed significantly among estuarine populations and within management regions. Additionally, all estuaries differed significantly in length distributions of Spotted Seatrout caught by the recreational fishery. Our results highlight the need to understand the relative contributions of estuary-specific data to regional assessment models if the four-region boundary scheme for Florida is continued. Moreover, as variation in growth and recruitment among estuaries likely reflects estuary-specific environmental conditions, we suggest that environmental variables be considered in future assessments and management of this recreationally valuable species.
Dimethyl fumarate, a two‐edged drug: Current status and future directions
Authors: Saidu Nathaniel Edward Bennett , Kavian Niloufar , Leroy Karen , Jacob Claus , Nicco Carole , Batteux Frédéric , Alexandre Jérôme 
Published: February 2019

AbstractDimethyl fumarate (DMF) is a fumaric acid ester registered for the treatment of relapsing‐remitting multiple sclerosis (RRMS). It induces protein succination leading to inactivation of cysteine‐rich proteins. It was first shown to possess cytoprotective and antioxidant effects in noncancer models, which appeared related to the induction of the nuclear factor erythroid 2 (NF‐E2)–related factor 2 (NRF2) pathway. DMF also displays antitumor activity in several cellular and mice models. Recently, we showed that the anticancer mechanism of DMF is dose‐dependent and is paradoxically related to the decrease in the nuclear translocation of NRF2. Some other studies performed indicate also the potential role of DMF in cancers, which are dependent on the NRF2 antioxidant and cellular detoxification program, such as KRAS‐mutated lung adenocarcinoma. It, however, seems that DMF has multiple biological effects as it has been shown to also inhibit the transcription factor nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB), thus blocking downstream targets that may be involved in the development and progression of inflammatory cascades leading to various disease processes, including tumors, lymphomas, diabetic retinopathy, arthritis, and psoriasis. Herein, we present the current status and future directions of the use of DMF in various diseases models with particular emphases on its targeting of specific intracellular signal transduction cascades in cancer; to shed some light on its possible mode of action.