bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cation homeostasis and transport related gene markers are differentially expressed in porcine buccal pouch mucosal cells during long-term cells primary culture in vitro
Authors: Bryja Artur , Dyszkiewicz-Konwińska Marta , Jankowski Maurycy , Celichowski Piotr , Stefańska Katarzyna , Chamier-Gliszczyńska Agata , Borowiec Blanka , Mehr Katarzyna , Bukowska Dorota , Antosik Paweł , Bruska Małgorzata , Zabel Maciej , Nowicki Michał , Kempisty Bartosz 
Published: January 2019

Abstract
The mucous membrane is composed of two layers. The layer of stratified squamous epithelium and the underlying layer of the connective tissue. The epithelium is composed of keratinocytes that are in different stages of differentiation, depending on their localization. In our research, after isolation of primary in vitro cultured buccal pouch mucosal cells, we observed keratinocytes in various stages of differentiation and fibroblasts. These cells, depending on the ionic dynamics, may be subject to different morphological and biochemical transformations. Understanding the expression profile of the normal oral mucosal tissue is important for further research into the effects of biomaterials on the mucosal cells, their growth, proliferation, and differentiation.
The porcine buccal pouch mucosal cells were used in this study. The oral mucosa was separated surgically and isolated enzymatically. The cells were in vitro cultured for 30 days, and after each step of in vitro culture (7 days, 15 days, 30 days), samples were collected for isolation of total RNA. The gene expression profile was measured using Affymetrix microarray assays.
In results, we observed genes belonging to two ontology groups: cation homeostasis and cation transport. These genes were up-regulated after 7 days of in vitro culture as compared to down-regulation after 15 and 30 days of in vitro culture. These results suggested that dynamic growth, proliferation and cell adhesion are more intense in the first 7 days of in vitro culture. We also observed, for the first time, the expression of ATP13A3 in porcine oral mucosal cells.
Pathogenesis and pathophysiology of ovarian follicular cysts in mammals
Authors: Rybska Marta , Knap Sandra , Jankowski Maurycy , Borowiec Blanka , Jeseta Michal , Bukowska Dorota , Antosik Paweł , Nowicki Michał , Zabel Maciej , Kempisty Bartosz , Jaśkowski Jędrzej M. 
Published: January 2019

Abstract
Ovarian cysts remain to be one of the most common and serious problems in reproduction of farm animals, as well as humans. Apart from causing the fall in reproductive potential of the ovaries, occupying the place in which folliculogenesis and oogenesis occur, they also cause hormone imbalances, by preventing corpus luteum formation, hence lowering the amount of steroid hormone production. While singular cysts rarely affect fertility, hormone fluctuations that are associated with their presence promotes their multiplication, which usually has more adverse effects. While the cysts are easily detectable in humans, possessing distinct echography while examined by ultrasound, multiple factors prevent widespread use of effective detection methods among large herds of farm animals. Because of lack of noticeable symptoms of early stages of such malignancies, they rarely get detected before the animal stops to exhibit symptoms of heat. That causes scientific research to be focused on not only methods of detection, but also the ways to negate the effects of ovarian cysts and bring the affected specimen back to reproductive potential. Despite that, high costs of diagnosis and treatment, cause them to be uncommon on commercial farms. As lack of fertility eliminates animals from breeding purposed herds, ovarian cysts persist as a cause of large losses of the animal husbandry business. Continuous research, focused on natural examples of ovarian cysts should be conducted, in order to improve methods of detection, prevention, treatment and recovery from the effects of ovarian cysts.
Transforming growth factor (TGF) – is it a key protein in mammalian reproductive biology?
Authors: Rybska Marta , Knap Sandra , Stefańska Katarzyna , Jankowski Maurycy , Chamier-Gliszczyńska Agata , Popis Małgorzata , Jeseta Michal , Bukowska Dorota , Antosik Paweł , Kempisty Bartosz , Jaśkowski Jędrzej M. 
Published: January 2019

Abstract
The superfamily of transforming growth factors β (TGF-β) consists of cytokines that are crucial in regulating the organism’s biological functions and includes three isoforms of TGF-β protein, Anti-Müllerian Hormone (AMH), inhibin A and B, activins, 20 bone morphogenetic proteins (BMP1-20) and 9 growth factors (GDF1-9). Their signal transduction pathway involves three types of membrane receptors that exhibit a serine/threonine kinase activity, as well as the Smad proteins. After ligand binding, the Smad proteins are phosphorylated and translocated to the nucleus, where they interact with transcription factors and affect gene expression. TGF-β family members are involved in cell growth and differentiation, as well as chemo-taxis and apoptosis, and play an important role during an inflammation. Defects in TGF-β proteins or in their signalling pathway underlie many severe diseases, such as systemic lupus, systemic scleroderma, bronchial asthma, atherosclerosis, hyperthyroidism or cancer. These factors are also crucial in mammal reproductive functions, as they are involved in folliculogenesis, steroidogenesis, ovulation, maternal-embryo interaction, embryo development and uterine decidualization. Their defects result in issues with fertility. This review focuses on the relevance of TGF-β family members in a mammal reproduction with an emphasis on three TGF-β isoforms, inhibins A and B, GDF-9 and their signal transduction pathway.
The Potential and Social Acceptability of Renewable Energy sources in North Iraq: Kurdistan Region
Authors: Morad Diler H. 
Published: January 2019

Kurdistan region government(KRG) is located to the north of Iraq, its Energy System has suffered from a lack of electricity, and many challenges to be overcome to meet future increases in demand for power, about 85% of the production of power is almost depended on fossils fuels, while the remaining 15% is covered by hydro-electrical plants. Therefore, the renewable energy sources are alternative sources of energy for reducing energy demand and CO2 emissions in KRG. In order to review the Kurdistan potential renewable energy and social-acceptance toward renewable energy source use and implementations, mixed methodology was used qualitative and quantitative to provide the most complete analysis of study problems. In this study, the potential renewable energy sources (RES) has been examined as well as the survey which was designed with three groups of questions: awareness of renewable energy sources, person background information and willingness to invest in renewable energy sources. The result showed that Kurdistan region has a great ability to utilize renewable energy sources and long-term economic viability of using RES in house wasn’t clear for 40% of 320 interviews. About 63% of the participants were ready to pay extra cost to get renewable energy. The majority of the participant think that public sector should take the first step towards renewable energy production as well as providing incentives and business models to motivate peoples to implement RES in their building.
Implementasi Program BMW (Berkas Mlaku Dewe) Dalam Meningkatkan Pelayanan Bidang Administrasi Di Kecamatan Sukodono Kabupaten Sidoarjo
Authors: Ma'rufah Kurnia Dwi , Sholichah Nihayatus 
Published: January 2019

Pelayanan publik merupakan pelayanan dasar dalam penyelenggaraan pemerintahan. Pelayanan publik sebagai indikator penting dalam penilaian kinerja pemerintah, baik di tingkat pusat maupun daerah. Kecamatan Sukodono merupakan salah satu wilayah di Kabupaten Sidoarjo yang padat penduduk. Kepadatan tersebut mengakibatkan waktu pelayanan yang cukup lama. Maka dari itu Kecamatan Sukodono melakukan inovasi pelayanan di bidang administrasi dengan memanfaatkan Elekronic Government dengan nama BMW (Berkas Mlaku Dewe).Penelitian ini menggunakan metode penelitian deskriptif dengan pendekatan kualitatif. Teknik pengumpulan data dalam penelitian ini menggunakan metode wawancara, observasi dan dokumentasi. Adapun fokus dalam penelitian ini adalah pelaksanaan program BMW menggunakan teori implementasi kebijakan. Teknik analisis data yaitu pengumpulan data, penyaringan data, penyajian data dan penarikan kesimpulan.Hasil penelitian menunjukkan adanya ruang pelayanan dengan fasilitas komputer, alat scan, lobi ber-AC dan jaringan internet di setiap desa. Desa Ngaresrejo adalah desa yang paling sering mengalami trouble jaringan. BMW memiliki dasar hukum yang diatur dalam Peraturan Bupati Nomor 52 Tahun 2016 Tentang Tata Naskah Dinas di Lingkungan Pemerintah Kabupaten Sidoarjo. Pelayanan BMW tidak dipungut biaya. Operator Desa dibekali dengan pelatihan yang dilakukan tiap dua minggu sekali secara rutin.Kesimpulannya bahwa adanya aplikasi BMW dapat diterima dengan baik oleh warga Sukodono karena proses pelayanan dapat dilakukan dengan cepat, dengan menggunakan sistem online yang menghubungkan antara desa dengan Kecamatan Sukodono yang dapat menghemat waktu, biaya dan tenaga. Walaupun dalam pelaksanaannya masih ditemukan beberapa kendala namun dapat diatasi dengan baik oleh Kecamatan Sukodono. Kata kunci: Implementasi kebijakan, pelayanan, BMW (Berkas Mlaku Dewe)
Correlation between Zinc Intake and Zinc Serum Levels with C-reactive Protein Level in Head and Neck Cancer Patients
Authors: Alam Muningtya Philiyanisa , Sunardi Diana , Rinaldi Ikhwan 
Published: January 2019

The inflammatory process of head and neck cancer leads to an increase the proinflammatory cytokines and the synthesis of c-reactive protein (CRP), which then causes metabolic alteration and anorexia in the patients. Zinc is one of nutrient that has an important role in suppressing inflammation, but it is reported that about 65% of head and neck cancer patients have zinc deficiency. The aim of this cross sectional study is to determine the correlation between zinc intake and serum zinc levels with CRP level as an effort in reducing inflammation process in head and neck cancer patients. Subjects were collected by consecutive sampling in the Oncology Polyclinic Rumah Sakit Kanker Dharmais, from 49 subjects 67,3% were men, most subjects were in the age range between 46–65 years. The highest frequency (65,3%) is nasopharyngeal cancer and 69,4% are already in stage IV. All subjects (100%) in this study have a zinc intake below the recommended dietary allowance (RDA) in Indonesia. The mean serum zinc level of the subjects was 9.83±2.62 μmol/L. Most subjects have elevated CRP levels. There was a weak negative correlation between zinc concentration and CRP levels of subjects (r =-0.292, p =0.042), but there was no correlation between zinc intake and CRP levels of subjects (r =-0.25).
Higher Nutritional Status of Lung Cancer Cachexia Patients is Associated with Higher Functional Capacity and Appetite
Authors: Sunardi Diana , Bardosono Saptawati 
Published: January 2019

Introduction: Lung cancer is highly prevalent and the major cause of cancer-related deaths worldwide. Severity of weight loss has been shown to have a negative impact on patients’ performance and quality of life. Early detection of anorexia and decline of functional capacity will be very usefull to prevent severe weight loss and a better prognosis. Methods: A cross sectional study was conducted in Dharmais Cancer Hospital. Subjects were lung cancer patients newly diagnosed or a minimal of 2 months pasca chemotherapy or radiotherapy, taken consecutively from inpatients and outpatients. Results: Subjects eligible for the study were 47, with a composition of 55.3% men and 44.7% women in the age range from 36–79 years old. There were significant relationship between body weight and BMI with ECOG score. Body weight, BMI and weight loss related significantly with VAS appetite and SNAQ score. These result indicate that subjects with a higher BMI and a lower weight loss have a better physical performa and appetite. There were significant relation between haemoglobin level, anemia status and serum albumin level with appetite and risk for anorexia. This result indicate that subjects with normal haemoglobin and albumin have a good appetite and a lower risk for anorexia. Conclusions: Positive prognosis of cancer patients can be obtained by maintaining BMI and preventing weight loss, since both affected the already known prognostic marker functional capacity significantly. Serum Albumin and hemoglobin are not only simple test, but also brings benefits, a clinical marker that may be used to assess the risk of anorexia.
Patriotisme Perempuan Sunda dalam Tari Ratu Graeni
Authors: Rosilawati Riyana , Mulyati Eti 
Published: January 2019

AbstractThis study aims to reveal the meaning of Ratu Graenidanceas a symbolic dance,i.e a Sundanese woman withher patriotic spirit. The dance deals with a woman who has a courageous spirit to overcome all problems, both internally and externally. The method employed is descriptive analysis through in-depth interviews and participant observation. The results are that from its performance form, Ratu Graenidancetells the story about the Queen who was preparing to practice a war with cingeus movement, which was disclosed through her head and body movements as a picture of the female characters who is agile and enthusiastic in facing various challenges of life. Her agility is also revealed in herfoot movements, such asmincid, trisik, and so forth. This is associated with the philosophical elements of the dance, which is useful for life. The meaning contained in this dance is that women can also play a role in a life, equally with men, with a sense of toughness, patriotic/struggle, and the perseverance behind the tenderness of a woman. Keywords: Patriotism, Sundanese woman, Ratu Graenidance AbstrakPenelitian ini bertujuan ingin mengungkapkan makna tari Ratu Graenisebagai tarian simbolik mengenai jiwa patriotisme seorang perempuan Sunda.Yakni, seorang perempuan yang mempunyai jiwa pemberani dalam mengatasi segala macam persoalan, baik secara internal maupun eksternal.Metode yang digunakanadalah kualitatif dengan pendekatan deskriptif analisis, yaitu melalui wawancara mendalam dan observasi berperanserta. Hasil yang diperoleh adalahdari segi bentuk pertunjukan, tari Ratu Graenimenggambarkan Sang Ratu yang sedang berlatih perangdengan menggunakan gerakan cingeus(gesit) yang ditarikandengan gerak kepala dan badan sebagai gambaran karakter perempuan yang gesit, penuh antusias dalam menghadapi berbagai tantangan kehidupan. Kegesitan terungkap pula dalam gerakannusuk, nangkis,kiprat soder,serta gerak kaki, sepertimincid, trisik, dan tincak tilu. Hal ini terkait dengan unsur filosofis dari tarian tersebut yang sangat bemanfaat bagi kehidupan. Makna yang terkandung dalam tarian ini bahwa wanita dapat berperan juga dalam kehidupan sejajar dengan kaum pria, yang sama-sama memiliki ketangguhan, berjiwa patriotik/perjuangan, dan kegigihan dibalik kelembutan seorang wanita. Kata kunci:Patriotisme, perempuan Sunda, tari Ratu Graeni
AVALIAÇÃO DA CONCENTRAÇÃO DE FLUORETO EM ÁGUA DE ABASTECIMENTO DE CENTROS MUNICIPAIS DE EDUCAÇÃO INFANTIL: UM ESTUDO EXPLORATÓRIO
Authors: Martorell Leandro Brambilla 
Published: January 2019

A concentração de fluoreto é um parâmetro importante para a avaliação da qualidade de fluoretação das águas de abastecimento, sendo o teor recomendado de 0,6 a 0,8 ppm. Objetivo: avaliar a concentração de fluoreto nas águas que abastecem dez Centros Municipais de Educação Infantil (CMEIs) no município de Anápolis. Métodos: avaliações realizadas por meio da análise do íon flúor nas amostras estudadas, utilizando colorímetro digital para as leituras. Resultados: Oito dos dez CMEIs apresentaram amostras dentro das especificações esperadas. Os dois CMEIs que apresentaram valores abaixo do recomendado utilizavam água proveniente de poço, cisterna ou fontes mistas. Conclusões: Os dados obtidos neste estudo reforçam a importância do monitoramento da concentração de fluoreto na água consumida em instituições de ensino, uma vez que a população infantil constitui grupo de risco para o desenvolvimento de fluorose dentária.
Palavra-Chave: Abastecimento de água, Fluoretação, Monitoramento.
Anafilaxia por clorhexidina en un contexto perioperatorio: diagnóstico y manejo
Authors: Calle Ana , Guevara-Saldaña Liliana , Cardona Ricardo 
Published: January 2019

Antecedentes: Durante una cirugía, el paciente está expuesto a múltiples medicamentos y moléculas que pueden asociarse con el desarrollo de hipersensibilidad, lo que dificulta la detección del agente causal de la reacción perioperatoria y hace necesaria la realización de pruebas guiadas por alergología.Reporte de caso: Hombre de 53 años quien posterior a una artroscopia de rodilla derecha se le administró ketorolaco endovenoso; a los 12 minutos se inició brote cutáneo pruriginoso en tórax, abdomen y extremidades; de inmediato se suspendió la infusión del medicamento y se administraron 100 mg de hidrocortisona intravenosa. A los 15 minutos presentó angioedema bilateral de párpados y sensación de disnea, por lo cual se aplicó oxígeno, 2 mg de clemastina intravenosa, 50 mg de ranitidina intravenosa y 20 μg de adrenalina subcutánea. Se realizó prueba epidérmica con clorhexidina a 0.5 % e inmunoglobulina E sérica específica para clorhexidina, ambas con resultados positivos. El paciente se recuperó sin complicaciones; al egreso se le prescribió etofenamato intramuscular.Conclusión: La anafilaxia perioperatoria es rara, pero potencialmente fatal. La identificación apropiada del medicamento o sustancia responsable de la reacción por medio de pruebas alergológicas disminuye las restricciones farmacológicas innecesarias y evita la reexposición.
Conocimiento de médicos de atención primaria sobre Rinitis Alérgica y su Impacto en el Asma (ARIA): estudio piloto comparativo Brasil, Paraguay, Uruguay
Authors: Urrutia-Pereira Marilyn , Fernandez Carolina , Valentin-Rostan Marylin , Cruz Álvaro , Torres Odete , Simon Laura , Nunes-Rinelli Pietro , Solé Dirceu 
Published: January 2019

Antecedentes: En ARIA se establecen directrices para el tratamiento de la rinitis alérgica y sus comorbilidades.Objetivo: Evaluar y comparar el nivel de conocimiento sobre rinitis alérgica y la guía ARIA en médicos de atención primaria (MAP) de Brasil, Paraguay y Uruguay.Métodos: 336 MAP (Brasil 109, Paraguay 127, Uruguay 100) respondieron un cuestionario sobre datos personales, profesionales y conocimiento en rinitis alérgica y ARIA; se utilizó Google Forms.Resultados: Predominaron las mujeres (73, 50.4 y 70 % en Brasil Paraguay Uruguay); 124 trabajaban en la comunidad y 75 en servicios de urgencias. Los MAP de Brasil y Uruguay reconocieron los síntomas de rinitis alérgica; 67 % de Uruguay no preguntaba sobre antecedentes de diagnóstico de rinitis alérgica, 93 % sobre diagnóstico de asma y 90 % sobre la interferencia de la rinitis con las actividades diarias. Uso de corticosteroides intranasales: 78, 92 y 54 % de los médicos de Brasil Paraguay y Uruguay; 100, 73.4 y 78 % de Paraguay, Brasil y Uruguay no derivaban el paciente al especialista; 26.6, 62 y 6 % de Brasil, Paraguay y Uruguay conocían ARIA.Conclusiones: La rinitis alérgica frecuentemente es atendida por el MAP, pero el reconocimiento de los síntomas y el conocimiento sobre ARIA son reducidos y el tratamiento no siempre es óptimo.
Prevalencia de asma, rinitis alérgica y dermatitis atópica en niños escolares en el Bajío de México
Authors: Ramírez-Soto Martin , Bedolla-Barajas Martín , González-Mendoza Tania 
Published: January 2019

Antecedentes: La prevalencia de enfermedades alérgicas varía notoriamente de acuerdo con la región geográfica.Objetivo: Determinar las prevalencias de asma, rinitis alérgica y dermatitis atópica en niños de seis y siete años residentes del Bajío, México.Métodos: Estudio transversal que incluyó una muestra de casi 15 mil niños en cinco ciudades del Bajío. Los padres contestaron el cuestionario de The International Study of Asthma and Allergies in Childhood, de donde se obtuvo la prevalencia de las enfermedades alérgicas y sus síntomas. Se estimaron intervalos de confianza (IC) a 95 % para proporciones.Resultados: Del total de la muestra, 7466 (52.5 %) fueron niños y 7463 (47.5 %) niñas. En general, las prevalencias de asma, asma con el ejercicio y asma actual fueron 6.1 % (IC 95 % = 5.7-6.5 ), 2.1 % (IC 95 % = 1.9-2.3) y 11.6 % (IC 95 % = 11.1-12.1), respectivamente. Por su parte, la prevalencia de rinitis alérgica fue de 5.4 % (IC 95 % (5.1-5.8 ), de rinitis más conjuntivitis de 19.2 % (IC 95 % = 18.6-19.8) y de rinitis actual de 52.8 % (IC 95 % = 52-53.6). La prevalencia de dermatitis atópica y de dermatitis actual fue de 3.4 % (IC 95 % = 3.1-3.7) y 7.8 % (IC 95 % = 7.4-8.2).Conclusiones: Los resultados indicaron que la prevalencia de las enfermedades alérgicas en niños escolares es similar a las observadas en estudios previos en México.
Pelatihan Pemeliharaan dan Penggunaan Drosophila Sebagai Bahan Praktikum Pada Guru Biologi SMA Di Lombok Barat
Authors: Bahri Syamsul , Sedijani Prapti , Zulkifli Lalu , Mertha I Gde 
Published: January 2019

Abstrak: Kegiatan pengabdian pada masyarakat ini secara umum bertujuan memberi keterampilan kepada guru-guru Biologi SMA dalam memelihara hewan percobaan Drosophila, serta teknik-teknik dasar yang diperlukan dalam menggunakan hewan ini sebagai bahan percobaan pada pokok-pokok bahasan yang terkait dengan mekanisme pewarisan sifat. Kegiatan pelatihan ini dilaksanakan di SMAN 2 Labu Api dan diikuti oleh 23 orang peserta yang merupakan guru-guru Biologi SMA yang tergabung dalam MGMP Biologi Lombok Barat. Materi pelatihan meliputi Teknik Kultur Drosophila, Teknik Isolasi Betina Virgin, Teknik Menyilangkan mutan Drosophila, dan Teknik Mengatasi Kontaminasi Kultur. Hasil observasi menunjukkan bahwa dari 23 orang peserta pelatihan, 96% peserta belum pernah menggunakan Drosophila dalam praktikum, dan 100% peserta tidak memiliki keterampilan memelihara dan menggunakan Drosophila sebagai bahan praktikum di sekolah tempat mereka bertugas. Monitoring dan diskusi selama 10 hari pasca pelatihan menunjukkan bahwa hampir 74% peserta telah mampu melakukan dengan baik teknik-teknik dasar yang diperlukan dalam kultur Drosophila. Materi pelatihan tersulit yang dialami peserta adalah Teknik Mengatasi Kontaminasi Medium Kultur. Hingga 10 hari pasca pelatihan tidak seorang pun peserta melaporkan keberhasilan merekan dalam mengerjakan teknik ini. Meskipun demikian pelatihan ini berhasil karena sebagian besar materi pelatihan mampu dikerjakan dengan baik oleh mayoritas peserta pelatihan. Kata Kunci: pelatihan, kultur Drosophila
ANALISIS KEMAMPUAN KREATIVITAS MAHASISWA FISIKA FKIP UNRAM DENGAN BERBANTUAN MULTIREPRENSENTASI DESIGN TES DALAM PENGUASAAN MATERI GELOMBANG DAN OPTIK
Authors: Kosim Kosim , Hikmawati Hikmawati , Mahrus Muh. 
Published: January 2019

Research on student creativity at The Study Program of Physics Education FKIP Universitas Mataram for Wave and Optics course was conducted. This study aims to describe student creativity in the creativity of solving problems related to wave and optical matter. Tests were given to 21 students as samples with a test format following the principle of multi-representation on wave and optical questions. This test emphasizes the creativity of students' cognitive thinking which includes fluency, flexibility, originality, and elaboration. The results of cognitive thinking creativity in problem solving creativity showed that 38% were good and 68% were quite. Thus the mapping of the level quality of cognitive thinking creativity the students of Physics Education at FKIP Unram in the odd semester of the 2018/2019 academic year is generally at the level of being quite creative.Keywords: cognitive thinking creativity, multi-representation