bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association analysis of SSTR2 copy number variation with cattle stature and its expression analysis in Chinese beef cattle
Authors: Cheng J. , Jiang R. , Cao X. K. , Liu M. , Huang Y. Z. , Lan X. Y. , Cao H. , Lei C. Z. , Chen H. 
Published: September 2019

AbstractCopy number variations (CNVs), as an important source of genetic variation, can affect a wide range of phenotypes by diverse mechanisms. The somatostatin receptor 2 (SSTR2) gene plays important roles in cell proliferation and apoptosis. Recently, this gene was mapped to a CNV region, which encompasses quantitative trait loci of cattle economic traits including body weight, marbling score, etc. Therefore,SSTR2CNV may exhibit phenotypic effects on cattle growth traits. In the current study, distribution ofSSTR2gene CNVs was investigated in six Chinese cattle breeds (XN, QC, NY, JA, LX and PN), and the results showed higher CNV polymorphisms in XN, QC and NY cattle. Next, association analysis between growth traits andSSTR2CNV was performed for XN, QC and NY cattle. In NY, individuals with fewer copies showed better performance than those with more copies. Further, the effects ofSSTR2CNV on theSSTR2mRNA level were also investigated, but revealed no significant correlation in either muscle or adipose tissue of adult NY cattle. The results suggested the potential for use ofSSTR2CNV as a marker for the molecular breeding of NY cattle.
Morphological and genetic variation of Wuchereria bancrofti microfilariae in carriers in Thailand, Lao PDR and Myanmar: evaluation using Giemsa-stained thick blood films
Authors: Jongthawin J. , Intapan P.M. , Thanchomnang T. , Sadaow L. , Laymanivong S. , Maleewong W. , Sanpool O. 
Published: September 2019

Abstract
There is geographical variation in the morphology and genetics of Wuchereria bancrofti, the major cause of human lymphatic filariasis. This study aims to compare morphological and genetic variation of W. bancrofti microfilariae recovered from carriers in Lao PDR, Myanmar and Thailand. Six morphological parameters (body length, cephalic space length and width, length of head to nerve ring, body width at nerve ring, Innenkȍrper length and number of column nuclei between the cephalic space and nerve ring) were evaluated from microfilariae in Giemsa-stained thick blood films. A portion of the cytochrome c oxidase subunit 1 gene of mitochondrial DNA was sequenced and analysed. Wuchereria bancrofti microfilariae showed a wide variation in their morphology and morphometry among three countries. Phylogenetic analysis confirmed that all microfilariae belonged to W. bancrofti. Higher mutation frequencies were observed in samples from Myanmar, relative to Thailand and Lao PDR. This study highlights the morphological disparities of microfilariae and genetic variability within W. bancrofti among three geographical locations. We found that reported morphometric differences between localities were less clear-cut than previously thought. Further studies are needed to determine the microfilarial periodicity in Lao PDR.
Biodiversity and spatio-temporal changes of benthic and demersal assemblages of circalittoral soft bottoms of the Bay of Málaga (northern Alboran Sea)
Authors: Moya-Urbano Elena , Urra Javier , Marina Pablo , Gallardo-núñez Marina , García Raso J. Enrique , Bárcenas Patricia , Serna Quintero José M. , Giráldez Ana , García Teresa , Baro Jorge , Rueda José L. 
Published: September 2019

AbstractThe Bay of Málaga is located in a high biodiversity and productivity area that harbours a wide variety of commercial species exploited by different fishing fleets. Benthic and demersal fauna from circalittoral soft bottoms have been studied using a benthic dredge (BD) (8 sampling stations) and an otter trawl (OT) (8 sampling stations on a seasonal basis). Some sediment and water column variables, as well as the trawling activity, have also been studied and used for analysing their linkage with the fauna. A total of 287 species have been found in these bottoms and fish, molluscs and crustaceans represented the most diverse and abundant faunistic groups. A new record of the decapod Hippolyte leptometrae for Spanish waters is also included in this study. Some multivariate analyses using BD samples indicated the presence of three assemblages, but these seem to represent different facies of a single benthic community due to the absence of acute sediment changes and significant differences in the fauna. OT samples only displayed differences related to seasons but not to sediment types or depth. These seasonal differences seem to be linked to biological and ecological features of both dominant and/or commercial species. Mud and organic matter contents (%OM) in sediment, as well as the temperature, were the main variables linked to the spatial distribution of the benthic community identified with BD, whereas medium and coarse sand as well as gravel contents were the main variables linked to the changes of the epibenthic and demersal assemblage resulting from OT samples. The information of this study is of importance for improving the knowledge on the biodiversity of circalittoral soft bottoms of the Mediterranean and Alboran Sea as well as for the potential creation of a Marine Fisheries Reserve in the Bay of Málaga.
The temporal aspects of mobility intentions: older people's reflections on present and future support arrangements
Authors: Barken Rachel 
Published: September 2019

AbstractDiscourses on later-life housing and care are polarised. Ageing in place – typically in one's long-term dwelling – is often presented as the most desirable living arrangement, while moving to a congregate environment tends to be regarded as a last resort. Such polarised discourses obscure older people's experiences as they contemplate needs for housing, health and social care. To expand current understandings of mobility intentions, this paper examines ‘time work’ – or actions undertaken to exert some agency over time – as older people with chronic health conditions and disabilities navigate present and future support arrangements. Based on an interpretive analysis of qualitative interviews with 22 older persons receiving home care in Ontario, Canada, I identify three themes that highlight the temporal aspects of mobility intentions: (a) maintaining continuity with the past and present, (b) constructing alternative futures and (c) facing precarity. Focusing on time work shows how people make sense of ageing in place and/or relocating not only in relation to their physical, social and psychological capacities, but also in relation to perceptions of the past, present and future. Time work, moreover, has implications for feelings of security in the present and a sense of control over the future. Based on these findings, I make suggestions for developing a comprehensive continuum of supports, so all older people can make meaningful choices concerning housing and care.
Exposure of humans to the zoonotic nematode Dirofilaria immitis in Northern Portugal
Authors: Fontes-Sousa A. P. , Silvestre-Ferreira A. C. , Carretón E. , Esteves-Guimarães J. , Maia-Rocha C. , Oliveira P. , Lobo L. , Morchón R. , Araújo F. , Simón F. , Montoya-Alonso J. A. 
Published: September 2019

Abstract
Dirofilariosis caused by Dirofilaria immitis (heartworm) is a zoonosis, considered an endemic disease of dogs and cats in several countries of Western Europe, including Portugal. This study assesses the levels of D. immitis exposure in humans from Northern Portugal, to which end, 668 inhabitants of several districts belonging to two different climate areas (Csa: Bragança, Vila Real and Csb: Aveiro, Braga, Porto, Viseu) were tested for anti-D. immitis and anti-Wolbachia surface proteins (WSP) antibodies. The overall prevalence of seropositivity to both anti-D. immitis and WSP antibodies was 6.1%, which demonstrated the risk of infection with D. immitis in humans living in Northern Portugal. This study, carried out in a Western European country, contributes to the characterisation of the risk of infection with D. immitis among human population in this region of the continent. From a One Health point of view, the results of the current work also support the close relationship between dogs and people as a risk factor for human infection
Blue Emitting BaAl2O4:Ce3+ Nanophosphors with High Color Purity and Brightness for White LEDs
Authors: Chatterjee Rituparna , Saha Subhajit , Panigrahi Karamjyoti , Ghorai Uttam Kumar , Das Gopes Chandra , Chattopadhyay Kalyan Kumar 
Published: September 2019

AbstractIn this work, strongly blue emitting Ce3+-activated BaAl2O4 nanophosphors were successfully synthesized by a sol–gel technique. The crystal structure, morphology, and microstructure of the nanophosphors have been studied by X-ray powder diffraction, field emission scanning electron microscopy, and high-resolution transmission electron microscopy. The photoluminescence spectra show the impact of concentration variation of Ce3+ on the photoluminescence emission of the phosphor. These nanophosphors display intense blue emission peaking at 422 nm generated by the Ce3+ 5d → 4f transition under 350 nm excitation. Our results reveal that this nanophosphor has the capability to take part in the emergent domain of solid-state lighting and field-emission display devices.
PAX5-induced upregulation of IDH1-AS1 promotes tumor growth in prostate cancer by regulating ATG5-mediated autophagy
Authors: Zhang Nan , Li Zhongyi , Bai Fuding , Zhang Shigeng 
Published: September 2019

AbstractProstate cancer (PCa) is one of the major malignancies affecting males’ health around the world. Long noncoding RNAs (lncRNAs), a class of long transcripts, has been reported as essential regulators in tumorigenesis. IDH1 antisense RNA 1 (IDH1-AS1) is an lncRNA which can interact with genes to regulate the Warburg effect. However, function and mechanism of it in tumorigenesis of PCa remains unclear. Therefore, our current study focused on exploring the role of IDH1-AS1 in PCa tumor growth. At first, the expression of IDH1-AS1 was identified to be upregulated in PCa samples and cell lines. Mechanism associated with the upregulation of IDH1-AS1 was analyzed and demonstrated by mechanism experiments. The result suggested that PAX5 is the transcriptional activator of IDH1-AS1. Functionally, loss-of function assays revealed that silencing of IDH1-AS1 inhibited cell proliferation and induced cell apoptosis both in vitro and in vivo. Through microarray analysis and Gene ontology (GO) analysis, we determined that IDH1-AS1 can affect PCa cell autophagy by upregulating ATG5 expression. Mechanism investigation further validated that IDH1-AS1 posttranscriptionally regulated ATG5 expression by enhancing the mRNA stability of ATG5 or upregulating ATG5 by sequestering miR-216b-5p. Consequently, rescue assays demonstrated that IDH1-AS1 promoted proliferation and apoptosis in PCa via ATG5-induced autophagy. Taken together, our study elucidated the function and regulatory mechanism of IDH1-AS1, thus providing a novel biomarker for PCa.
Prevotella copri is associated with carboplatin-induced gut toxicity
Authors: Yu Chaoheng , Zhou Bailing , Xia Xuyang , Chen Shuang , Deng Yun , Wang Yantai , Wu Lei , Tian Yaomei , Zhao Binyan , Xu Heng , Yang Li 
Published: September 2019

AbstractAs a widely used cancer drug, carboplatin often results in serious side effects, such as gut toxicity. In this study, we examined the effects of gut microbiota on mice with carboplatin-induced intestinal mucosal damage. Carboplatin resulted in intestinal mucositis, as indicated by weight loss, diarrhoea, and infiltration of inflammatory cells. It markedly increased the expression of inflammatory cytokines/chemokines in intestine. Carboplatin also altered the diversity and composition of the gut microbiota. A significantly higher abundance of Prevotella copri (P. copri) was observed in carboplatin-treated mice. Moreover, the content of P. copri was positively correlated with the severity of intestinal mucositis. Pretreatment with metronidazole reduced the content of P. copri and relieved the intestinal mucosal injury and inflammation that was induced by carboplatin. Further study revealed that supplementation with P. copri in carboplatin-treated mice resulted in more severe tissue damage, lower tight junction protein expression and higher cytokine expression, and it enhanced both local and systemic immune responses. These data demonstrated that P. copri was involved in the pathological process of carboplatin-induced intestinal mucositis, suggesting a potential attenuation of carboplatin-induced intestinal mucositis by targeting P. copri.
Targeting a moonlighting function of aldolase induces apoptosis in cancer cells
Authors: Gizak Agnieszka , Wiśniewski Janusz , Heron Paul , Mamczur Piotr , Sygusch Jurgen , Rakus Dariusz 
Published: September 2019

AbstractMuscle fructose-1,6-bisphosphate aldolase (ALDOA) is among the most abundant glycolytic enzymes in all cancer cells. Here, we show that the enzyme plays a previously unknown and critical role in a cancer cell survival. Simultaneous inhibition of ALDOA activity and interaction with F-actin cytoskeleton using ALDOA slow-binding inhibitor UM0112176 leads to a rapid cofilin-dependent loss of F-actin stress fibers which is associated with elevated ROS production, inhibition of ATP synthesis, increase in calcium levels, caspase activation and arrested cellular proliferation. These effects can be reproduced by silencing of ALDOA. The mechanism of pharmacological action is, however, independent of the catalytic function of the enzyme, specific to cancer cells, and is most deleterious to cells undergoing the epithelial–mesenchymal transition, a process facilitating cancer cell invasion. Our results demonstrate that the overabundance of ALDOA in cancer cells is associated with its moonlighting rather than catalytic functions. This may have significant implications for development of novel broad-based anti-cancer therapies.
An anti-CD103 antibody-drug conjugate prolongs the survival of pancreatic islet allografts in mice
Authors: Xue Da , Liu Pili , Chen Wangming , Zhang Chi , Zhang Lei 
Published: September 2019

AbstractCD103 mediates T-cell infiltration and organ allograft rejection, and depletion of CD103-expressing cells is a promising therapeutic strategy for allograft intolerance. Recently, we verified that M290-MC-MMAF, an anti-CD103 antibody-drug conjugate, potently eliminates CD103-positive cells in vivo, with high specificity and minimal toxicity. However, the contribution of M290-MC-MMAF to blocking the CD103/E-cadherin pathway involved in transplant rejection remains unclear. Herein, we examined the impact of systemic administration of M290-MC-MMAF on allografts in an islet transplantation model. M290-MC-MMAF treatment maintained the long-term survival of islet allografts (>60 days) compared to mock injection or unconjugated M290 antibody treatment (<18 days). The change was associated with a decrease in CD103+CD8+effector T cells and an increase in CD4+CD25+regulatory T cells. CD103+CD8+effector T-cell transfer or CD4+CD25+regulatory T-cell depletion resulted in a rapid loss of allografts in long-surviving islet hosts. Moreover, M290-MC-MMAF treatment reduced IL-4, IL-6, and TNF-α expression levels and increased IL-10 expression in the grafts, which presented an immunosuppressive cytokine profile. In conclusion, targeting CD103 with M290-MC-MMAF induced immunosuppression and prolonged the survival of pancreatic islet allografts in mice, indicating the potential clinical value of M290-MC-MMAF in therapeutic interventions for allograft rejection.
Free-space transfer of comb-rooted optical frequencies over an 18 km open-air link
Authors: Kang Hyun Jay , Yang Jaewon , Chun Byung Jae , Jang Heesuk , Kim Byung Soo , Kim Young-Jin , Kim Seung-Woo 
Published: September 2019

AbstractPhase-coherent transfer of optical frequencies over a long distance is required for diverse photonic applications, including optical clock dissemination and physical constants measurement. Several demonstrations were made successfully over fiber networks, but not much work has been done yet through the open air where atmospheric turbulence prevails. Here, we use an 18 km outdoor link to transmit multiple optical carriers extracted directly from a frequency comb of a 4.2 THz spectral width. In stabilization to a high-finesse cavity with a 1.5 Hz linewidth, the comb-rooted optical carriers are simultaneously transferred with collective suppression of atmospheric phase noise to −80 dBc Hz−1. Microwaves are also delivered by pairing two separate optical carriers bound with inter-comb-mode coherence, for example a 10 GHz signal with phase noise of −105 dBc Hz−1 at 1 Hz offset. Lastly, an add-on demonstration is given for multi-channel coherent optical communications with the potential of multi-Tbps data transmission in free space.
Anchoring Cu1 species over nanodiamond-graphene for semi-hydrogenation of acetylene
Authors: Huang Fei , Deng Yuchen , Chen Yunlei , Cai Xiangbin , Peng Mi , Jia Zhimin , Xie Jinglin , Xiao Dequan , Wen Xiaodong , Wang Ning , Jiang Zheng , Liu Hongyang , Ma Ding 
Published: September 2019

AbstractThe design of cheap, non-toxic, and earth-abundant transition metal catalysts for selective hydrogenation of alkynes remains a challenge in both industry and academia. Here, we report a new atomically dispersed copper (Cu) catalyst supported on a defective nanodiamond-graphene (ND@G), which exhibits excellent catalytic performance for the selective conversion of acetylene to ethylene, i.e., with high conversion (95%), high selectivity (98%), and good stability (for more than 60 h). The unique structural feature of the Cu atoms anchored over graphene through Cu-C bonds ensures the effective activation of acetylene and easy desorption of ethylene, which is the key for the outstanding activity and selectivity of the catalyst.
Molecular Profiles and Metastasis Markers in Chinese Patients with Gastric Carcinoma
Authors: Chen Chao , Shi Chunmei , Huang Xiaochun , Zheng Jianwei , Zhu Zhongyi , Li Qiaolian , Qiu Si , Huang Zhiqing , Zhuang Zhenkun , Wu Riping , Liu Panhong , Wu Fan , Lin Shanyun , Li Bo , Zhang Xiuqing , Chen Qiang 
Published: September 2019

AbstractThe goal of this work was to investigate the molecular profiles and metastasis markers in Chinese patients with gastric carcinoma (GC). In total, we performed whole exome sequencing (WES) on 74 GC patients with tumor and adjacent normal formalin-fixed, paraffin-embedded (FFPE) tissue samples. The mutation spectrum of these samples showed a high concordance with TCGA and other studies on GC. PTPRT is significantly associated with metastasis of GC, suggesting its predictive role in metastasis of GC. Patients carrying BRCA2 mutations tend not to metastasize, which may be related to their sensitivity to chemotherapy. Mutations in MACF1, CDC27, HMCN1, CDH1 and PDZD2 were moderately enriched in peritoneal metastasis (PM) samples. Furthermore, we found two genomic regions (1p36.21 and Xq26.3) were associated with PM of GC, and patients with amplification of 1p36.21 and Xq26.3 have a worse prognosis (P = 0.002, 0.01, respectively). Our analysis provides GC patients with potential markers for single and combination therapies.
Modular network for high-rate quantum conferencing
Authors: Ottaviani Carlo , Lupo Cosmo , Laurenza Riccardo , Pirandola Stefano 
Published: September 2019

AbstractOne of the main open problems in quantum communication is the design of efficient quantum-secured networks. This is a challenging goal, because it requires protocols that guarantee both unconditional security and high communication rates, while increasing the number of users. In this scenario, continuous-variable systems provide an ideal platform where high rates can be achieved by using off-the-shelf optical components. At the same time, the measurement-device independent architecture is also appealing for its feature of removing a substantial portion of practical weaknesses. Driven by these ideas, here we introduce a modular design of continuous-variable network where each individual module is a measurement-device-independent star network. In each module, the users send modulated coherent states to an untrusted relay, creating multipartite secret correlations via a generalized Bell detection. Using one-time pad between different modules, the network users may share a quantum-secure conference key over arbitrary distances at constant rate.
Liquorpunktion – Schritt für Schritt
Authors: Siedler Gabriela , Kallmünzer Bernd , Schwab Stefan 
Published: September 2019

AbstractPerforming a diagnostic lumbar puncture is an essential clinical skill for the clinician. The procedure is of high importance for both, the emergency care for patients with suspected infection of the cerebral nervous system or subarachnoidal hemorrhage, as well as the elective diagnostic work up of chronic disease including multiple sclerosis and dementia. Furthermore the technique’s principles are applied for spinal anesthesia or the intrathecal administration of drugs. This article describes a Standard Operating Procedure for the lumbar puncture step by step. Based on the latest evidence, it particularly focusses on clinically relevant questions and practical aspects.
Community Service as Patientsʼ Tutor to Avoid Delirium
Authors: Prokop Axel , Reinauer Karl Michael , Koebler Manfred , Schwerb Erna , Keller Lisa , Chmielnicki Marc 
Published: September 2019

Abstract
Background Today, nearly two thirds of inpatients in trauma surgery hospitals are over 70 years old and at significant risk for comorbidities. These patients frequently suffer delirium. Delirium occurs in 15 – 30% of these trauma patients and increases the risk of mortality in up to 15 – 25% of cases. Conversation, attention, and activity significantly reduce this risk.
Objectives Thus, the Centre for Geriatric Traumatology and the District Seniors Council have initiated a new project of a visiting service, to prevent delirium and anxiety conditions in elderly inpatients. Volunteers from the welfare program offer daily care for selected patients.
Methods They care for and interact with the patients for approximately one hour per day. They accompany the patients in the hospital and during mobilization, explain, read aloud, converse, or simply listen, and thus provide calm, support, and company. The attendants are volunteers who have been previously trained by the clinic, who regularly attend team meetings and draw up reports from each admission. All patients and relatives are informed prior to these visits and are notified with an accompanying letter. There are structured visiting plans for each week.
Results From July 17 to May 19, 4031 patients in our clinic and in three other hospitals have been accompanied in the program. None of the patients suffered delirium. The patients and attendants found the care very good and personally satisfying. Evaluations of the volunteers for the success of their work, using the German school-grading system, was an average 1.3. Noticeable was the markedly reduced frequency of patient calls to nursing, who were thus relieved. Travel expenses and insurance costs for the volunteers were reimbursed.
Conclusion In times of skilled labour shortages, patient support provided by volunteer patient attendants enables innovative care for patients at risk for delirium. The assistance of the attendants alone reduced the workload on the nursing staff.
„Von Motivation und Sortierschachteln“
Authors: Hertlein Margit 
Published: September 2019

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