bluuebunny/crossref_metadata_2025_split huggingface dataset:
The dyspnoea–inactivity vicious circle in COPD: development and external validation of a conceptual model
Authors: Ramon Maria A. , Ter Riet Gerben , Carsin Anne-Elie , Gimeno-Santos Elena , Agustí Alvar , Antó Josep M. , Donaire-Gonzalez David , Ferrer Jaume , Rodríguez Esther , Rodriguez-Roisin Robert , Puhan Milo A. , Garcia-Aymerich Judith 
Published: August 2018

The vicious circle of dyspnoea–inactivity has been proposed, but never validated empirically, to explain the clinical course of chronic obstructive pulmonary disease (COPD). We aimed to develop and validate externally a comprehensive vicious circle model.We utilised two methods. 1) Identification and validation of all published vicious circle models by a systematic literature search and fitting structural equation models to longitudinal data from the Spanish PAC-COPD (Phenotype and Course of COPD) cohort (n=210, mean age 68 years, mean forced expiratory volume in 1 s (FEV1) 54% predicted), testing both the hypothesised relationships between variables in the model (“paths”) and model fit. 2) Development of a new model and external validation using longitudinal data from the Swiss and Dutch ICE COLD ERIC (International Collaborative Effort on Chronic Obstructive Lung Disease: Exacerbation Risk Index Cohorts) cohort (n=226, mean age 66 years, mean FEV157% predicted).We identified nine vicious circle models for which structural equation models confirmed most hypothesised paths but showed inappropriate fit. In the new model, airflow limitation, hyperinflation, dyspnoea, physical activity, exercise capacity and COPD exacerbations remained related to other variables and model fit was appropriate. Fitting it to ICE COLD ERIC, all paths were replicated and model fit was appropriate.Previously published vicious circle models do not fully explain the vicious circle concept. We developed and externally validated a new comprehensive model that gives a more relevant role to exercise capacity and COPD exacerbations.
microRNA–mRNA regulatory networks underlying chronic mucus hypersecretion in COPD
Authors: Tasena Hataitip , Faiz Alen , Timens Wim , Noordhoek Jacobien , Hylkema Machteld N. , Gosens Reinoud , Hiemstra Pieter S. , Spira Avrum , Postma Dirkje S. , Tew Gaik W. , Grimbaldeston Michele A. , van den Berge Maarten , Heijink Irene H. , Brandsma Corry-Anke 
Published: August 2018

Chronic mucus hypersecretion (CMH) is a common feature in chronic obstructive pulmonary disease (COPD) and is associated with worse prognosis and quality of life. This study aimed to identify microRNA (miRNA)–mRNA regulatory networks underlying CMH.The expression profiles of miRNA and mRNA in bronchial biopsies from 63 COPD patients were associated with CMH using linear regression. Potential mRNA targets of each CMH-associated miRNA were identified using Pearson correlations. Gene set enrichment analysis (GSEA) and STRING (search tool for the retrieval of interacting genes/proteins) analysis were used to identify key genes and pathways.20 miRNAs and 539 mRNAs were differentially expressed with CMH in COPD. The expression of 10 miRNAs was significantly correlated with the expression of one or more mRNAs. Of these, miR-134-5p, miR-146a-5p and the let-7 family had the highest representation of CMH-associated mRNAs among their negatively correlated predicted targets.KRASandEDN1were identified as key regulators of CMH and were negatively correlated predicted targets of miR-134-5p and let-7a-5p, let-7d-5p, and let-7f-5p, respectively. GSEA suggested involvement ofMUC5AC-related genes and several other relevant gene sets in CMH. The lower expression of miR-134-5p was confirmed in primary airway fibroblasts from COPD patients with CMH.We identified miR-134-5p, miR-146a-5p and let-7 family, along with their potential target genes includingKRASandEDN1, as potential key miRNA–mRNA networks regulating CMH in COPD.
RETRACTED ARTICLE: Reprogramming glioblastoma multiforme cells into neurons by protein kinase inhibitors
Authors: Yuan Jie , Zhang Fan , Hallahan Dennis , Zhang Zhen , He Liming , Wu Ling-Gang , You Meng , Yang Qin 
Published: August 2018

Abstract
Background
Reprogramming of cancers into normal-like tissues is an innovative strategy for cancer treatment. Recent reports demonstrate that defined factors can reprogram cancer cells into pluripotent stem cells. Glioblastoma multiforme (GBM) is the most common and aggressive malignant brain tumor in humans. Despite multimodal therapy, the outcome for patients with GBM is still poor. Therefore, developing novel therapeutic strategy is a critical requirement.

Methods
We have developed a novel reprogramming method that uses a conceptually unique strategy for GBM treatment. We screened a kinase inhibitor library to find which candidate inhibitors under reprogramming condition can reprogram GBM cells into neurons. The induced neurons are identified whether functional and loss of tumorigenicity.

Results
We have found that mTOR and ROCK kinase inhibitors are sufficient to reprogram GBM cells into neural-like cells and “normal” neurons. The induced neurons expressed neuron-specific proteins, generated action potentials and neurotransmitter receptor-mediated currents. Genome-wide transcriptional analysis showed that the induced neurons had a profile different from GBM cells and were similar to that of control neurons induced by established methods. In vitro and in vivo tumorigenesis assays showed that induced neurons lost their proliferation ability and tumorigenicity. Moreover, reprogramming treatment with ROCK-mTOR inhibitors prevented GBM local recurrence in mice.

Conclusion
This study indicates that ROCK and mTOR inhibitors-based reprogramming treatment prevents GBM local recurrence. Currently ROCK-mTOR inhibitors are used as anti-tumor drugs in patients, so this reprogramming strategy has significant potential to move rapidly toward clinical trials.
The Relation Between Thyroid Function and Anemia: A Pooled Analysis of Individual Participant Data
Authors: Wopereis Daisy M , Du Puy Robert S , van Heemst Diana , Walsh John P , Bremner Alexandra , Bakker Stephan J L , Bauer Douglas C , Cappola Anne R , Ceresini Graziano , Degryse Jean , Dullaart Robin P F , Feller Martin , Ferrucci Luigi , Floriani Carmen , Franco Oscar H , Iacoviello Massimo , Iervasi Georgio , Imaizumi Misa , Jukema J Wouter , Khaw Kay-Tee , Luben Robert N , Molinaro Sabrina , Nauck Matthias , Patel Kushang V , Peeters Robin P , Psaty Bruce M , Razvi Salman , Schindhelm Roger K , van Schoor Natasja M , Stott David J , Vaes Bert , Vanderpump Mark P J , Völzke Henry , Westendorp Rudi G J , Rodondi Nicolas , Cobbaert Christa M , Gussekloo Jacobijn , den Elzen Wendy P J ,   Thyroid Studies Collaboration
Published: August 2018

Abstract

Context
Anemia and thyroid dysfunction often co-occur, and both increase with age. Human data on relationships between thyroid disease and anemia are scarce.

Objective
To investigate the cross-sectional and longitudinal associations between clinical thyroid status and anemia.

Design
Individual participant data meta-analysis.

Setting
Sixteen cohorts participating in the Thyroid Studies Collaboration (n = 42,162).

Main Outcome Measures
Primary outcome measure was anemia (hemoglobin &lt;130 g/L in men and &lt;120 g/L in women).

Results
Cross-sectionally, participants with abnormal thyroid status had an increased risk of having anemia compared with euthyroid participants [overt hypothyroidism, pooled OR 1.84 (95% CI 1.35 to 2.50), subclinical hypothyroidism 1.21 (1.02 to 1.43), subclinical hyperthyroidism 1.27 (1.03 to 1.57), and overt hyperthyroidism 1.69 (1.00 to 2.87)]. Hemoglobin levels were lower in all groups compared with participants with euthyroidism. In the longitudinal analyses (n = 25,466 from 14 cohorts), the pooled hazard ratio for the risk of development of anemia was 1.38 (95% CI 0.86 to 2.20) for overt hypothyroidism, 1.18 (1.00 to 1.38) for subclinical hypothyroidism, 1.15 (0.94 to 1.42) for subclinical hyperthyroidism, and 1.47 (0.91 to 2.38) for overt hyperthyroidism. Sensitivity analyses excluding thyroid medication or high levels of C-reactive protein yielded similar results. No differences in mean annual change in hemoglobin levels were observed between the thyroid hormone status groups.

Conclusion
Higher odds of having anemia were observed in participants with both hypothyroid function and hyperthyroid function. In addition, reduced thyroid function at baseline showed a trend of increased risk of developing anemia during follow-up. It remains to be assessed in a randomized controlled trial whether treatment is effective in reducing anemia.
Cortisol Excess in Patients With Primary Aldosteronism Impacts Left Ventricular Hypertrophy
Authors: Adolf Christian , Köhler Anton , Franke Anna , Lang Katharina , Riester Anna , Löw Anja , Heinrich Daniel A , Bidlingmaier Martin , Treitl Marcus , Ladurner Roland , Beuschlein Felix , Arlt Wiebke , Reincke Martin 
Published: August 2018

Abstract

Context
Primary aldosteronism (PA) represents the most frequent form of endocrine hypertension. Hyperaldosteronism and hypercortisolism both induce excessive left ventricular hypertrophy (LVH) compared with matched essential hypertensives. In recent studies frequent cosecretion of cortisol and aldosterone has been reported in patients with PA.

Objective
Our aim was to investigate the impact of cortisol cosecretion on LVH in patients with PA. We determined 24-hour excretion of mineralocorticoids and glucocorticoids by gas chromatography–mass spectrometry and assessed cardiac remodeling using echocardiography initially and 1 year after initiation of treatment of PA.

Patients
We included 73 patients from the Munich center of the German Conn’s registry: 45 with unilateral aldosterone-producing adenoma and 28 with bilateral adrenal hyperplasia.

Results
At the time of diagnosis, 85% of patients with PA showed LVH according to left ventricular mass index [(LVMI); median 62.4 g/m2.7]. LVMI correlated positively with total glucocorticoid excretion (r2 = 0.076, P = 0.018) as well as with tetrahydroaldosterone excretion (r2 = 0.070, P = 0.024). Adrenalectomy led to significantly reduced LVMI in aldosterone-producing adenoma (P &lt; 0.001) whereas mineralocorticoid receptor antagonist therapy in bilateral adrenal patients with hyperplasia reduced LVMI to a lesser degree (P = 0.024). In multivariate analysis, the decrease in LVMI was positively correlated with total glucocorticoid excretion and systolic 24-hour blood pressure, but not with tetrahydroaldosterone excretion.

Conclusion
Cortisol excess appears to have an additional impact on cardiac remodeling in patients with PA. Treatment of PA by either adrenalectomy or mineralocorticoid receptor antagonist improves LVMI. This effect was most pronounced in patients with high total glucocorticoid excretion.
Transcriptional and Epigenetic Changes Influencing Skeletal Muscle Metabolism in Women With Polycystic Ovary Syndrome
Authors: Nilsson Emma , Benrick Anna , Kokosar Milana , Krook Anna , Lindgren Eva , Källman Thomas , Martis Mihaela M , Højlund Kurt , Ling Charlotte , Stener-Victorin Elisabet 
Published: August 2018

AbstractContextInsulin resistance in skeletal muscle is a major risk factor for the development of type 2 diabetes in women with polycystic ovary syndrome (PCOS). Despite this, the mechanisms underlying insulin resistance in PCOS are largely unknown.ObjectiveTo investigate the genome-wide DNA methylation and gene expression patterns in skeletal muscle from women with PCOS and controls and relate them to phenotypic variations.Design/ParticipantsIn a case-control study, skeletal muscle biopsies from women with PCOS (n = 17) and age-, weight-, and body mass index‒matched controls (n = 14) were analyzed by array-based DNA methylation and mRNA expression profiling.ResultsEighty-five unique transcripts were differentially expressed in muscle from women with PCOS vs controls, including DYRK1A, SYNPO2, SCP2, and NAMPT. Furthermore, women with PCOS had reduced expression of genes involved in immune system pathways. Two CpG sites showed differential DNA methylation after correction for multiple testing. However, an mRNA expression of ∼30% of the differentially expressed genes correlated with DNA methylation levels of CpG sites in or near the gene. Functional follow-up studies demonstrated that KLF10 is under transcriptional control of insulin, where insulin promotes glycogen accumulation in myotubes of human muscle cells. Testosterone downregulates the expression levels of COL1A1 and MAP2K6.ConclusionPCOS is associated with aberrant skeletal muscle gene expression with dysregulated pathways. Furthermore, we identified specific changes in muscle DNA methylation that may affect gene expression. This study showed that women with PCOS have epigenetic and transcriptional changes in skeletal muscle that, in part, can explain the metabolic abnormalities seen in these women.
Facile Preparation of a Novel Transparent Composite Film Based on Bacterial Cellulose and Atactic Polypropylene
Authors: Wang Qidong , Asoh Taka-Aki , Uyama Hiroshi 
Published: August 2018

Abstract
A transparent bacterial cellulose/atactic polypropylene (BC/at-PP) composite film was prepared by a very facile “sandwich” hot-press method. The resulting BC/at-PP composite film exhibited good transparency, hydrophobicity, and significantly enhanced mechanical properties, indicating its tremendous potential as transparent film for food packaging and optoelectronics applications.
The dynamic side of the Warburg effect: glycolytic intermediates as buffer for fluctuating glucose and O2 supply in tumor cells
Authors: van Beek Johannes H.G.M. 
Published: August 2018

Background:Tumor cells show the Warburg effect: high glucose uptake and lactate production despite sufficient oxygen supply. Otto Warburg found this effect in tissue slices and in suspensions of Ehrlich ascites tumor cells. Remarkably, these ascites tumor cells can transiently take up glucose an order of magnitude faster than the steady high rate measured by Warburg for hours.Methods:The purpose of the transiently very high glucose uptake is investigated here with a computational model of glycolysis, oxidative phosphorylation and ATP consumption which reproduces short kinetic experiments on the ascites tumor cells as well as the long-lasting Warburg, Crabtree and Pasteur effects. The model, extended with equations for glucose and O2 transport in tissue, is subsequently used to predict metabolism in tumor cells during fluctuations of tissue blood flow resulting in cycling hypoxia.Results:The model analysis suggests that the head section of the glycolytic chain in the tumor cells is partially inhibited in about a minute when substantial amounts of glucose have been taken up intracellularly; this head section of the glycolytic chain is subsequently disinhibited slowly when concentrations of glycolytic intermediates are low. Based on these dynamic characteristics, simulations of tissue with fluctuating O2and glucose supply predict that tumor cells greedily take up glucose when this periodically becomes available, leaving very little for other cells. The glucose is stored as fructose 1,6-bisphosphate and other glycolytic intermediates, which are used for ATP production during O2and glucose shortages.Conclusions:The head section of glycolysis which phosphorylates glucose may be dynamically regulated and takes up glucose at rates exceeding the Warburg effect if glucose levels have been low for some time. The hypothesis is put forward here that dynamic regulation of the powerful glycolytic enzyme system in tumors is used to buffer oxygen and nutrient fluctuations in tissue.
Embedding a user-centred approach in the development of complex behaviour change intervention to improve outcomes for young adults living with type 1 diabetes: The D1 Now Study
Authors: Walsh Deirdre M.J. , Hynes Lisa , O'Hara Mary Clare , McSharry Jenny ,   D1 Now Young Adult Panel, Dinneen Séan F. , Byrne Molly 
Published: August 2018

Background: Type 1 diabetes (T1D) is an auto-immune condition which requires intensive self-management. Diabetes self-management is challenging, especially during young adulthood. Effective interventions to improve outcomes for young adults (18-30 year olds) with T1D are needed. This paper describes the development of the D1 Now intervention, employing a user-centred approach to engage with stakeholders in parallel with the application of theory. Methods: Intervention development consisted of 4 phases: 1) the formation of a public and patient involvement (PPI) Young Adult Panel (YAP); 2) a systematic review to synthesise evidence regarding the effectiveness of interventions aimed at improving outcomes for young adults with T1D; 3) understand young adults’ diabetes self-management behaviour through engagement with key stakeholders; and 4) an expert consensus meeting to discuss self-management strategies identified in Phase 1 and 3 that would form the core components of the D1 Now intervention. Results: The YAP resulted in meaningful involvement between young adults, researchers and service providers. The systematic review highlighted a lack of quality intervention studies. Qualitative findings highlighted how young adult self-management is driven by complex interactions between external resources, which influence capability, and motivation. The expert panel in Phase 4 highlighted focus areas to improve outcomes for young adults and implementation strategies. Subsequent to these 4 phases, 3 intervention components have been identified: 1) a key worker to liaise with the young adult; 2) an online portal to facilitate relationship building between staff and young adults; and 3) an agenda setting tool to facilitate joint decision-making. Conclusions: This study described the systematic development of an intervention underpinned by theoretical frameworks and PPI, and has identified components for the D1 Now intervention. The resulting intervention content will now be subject to an intervention optimisation process.
Assessment of health behaviors, nutritional behaviors, and self-efficacy in patients with morbid obesity
Authors: Sekuła Marzena , Boniecka Iwona , Paśnik Krzysztof 
Published: August 2018

Cel pracyCelem badania była ocena nasilenia zachowań zdrowotnych i żywieniowych, dotyczących jedzenia emocjonalnego, nawykowego, stosowania restrykcji dietetycznych, określenie poczucia własnej skuteczności pacjentów z otyłością olbrzymią oraz sprawdzenie związków pomiędzy wskaźnikiem masy ciała BMI badanych pacjentów a ich zachowaniami żywieniowymi i poczuciem skuteczności.MetodaBadaniem objęto 37 chorych ze zdiagnozowaną otyłością II i III stopnia w wieku od 18-62 lat (M=39,94; SD=12,20). Wskaźnik masy ciała BMI badanych wynosił od 36 do 60kg/m2 (M=43,50; SD=5,36). Zastosowano narzędzia badawcze: Inwentarz Zachowań Żywieniowych (IZZ), Kwestionariusz Zachowań Związanych z Jedzeniem (KZZJ) i Skalę Uogólnionej Własnej Skuteczności (GSES).WynikiBadanie wykazało, że pacjenci z otyłością olbrzymią charakteryzowali się przeciętnym w przypadku mężczyzn (5 stenów) i niskim w przypadku kobiet (4 steny) nasileniem zachowań zdrowotnych. Badani przejawiali tendencję do nieprawidłowych zachowań żywieniowych, jedzenia emocjonalnego, nawykowego i stosowania restrykcji dietetycznych. Uogólnione poczucie własnej skuteczności GSES w badanej grupie było na poziomie od 4 do 10 stenów, a średnia na poziomie wysokim (7 stenów).WnioskiChorzy z otyłością olbrzymią powinni zostać objęci szeroko rozumianą edukacją w obszarze zdrowotnym, żywieniowym oraz psychologicznym.
Applying of Neuman’s Systems Model in the course of nursing care of a patient who has Cervical Disc Herniation treatment<p>Neuman Sistemler Modelinin servikal disk herni tedavisi gören bir hastanın hemşirelik bakımı sürecinde kullanılması
Authors: Eren Handan , Çalışkan Nurcan 
Published: August 2018

Nursing is a professional health discipline and nursing models based on nursing theories form its scientific foundations. Neuman’s Systems Model is one of these models. The model focuses on the individual's well-being and is based on holistic nursing care. In the model, the individual is an open system interpreting the stressors he perceives. By defining these stressors, the nurse develops the defense mechanisms of the individual on the primary, secondary and tertiary level. The nurse is responsible for the planning, implementation and evaluation. The cervical disc hernia that affects life activities is helped cope with the stressors perceived by the individual receiving the diagnosis. Thus, the quality of life of the individual can be increased, the treatment process can be shortened and the cost can be reduced. In this study, the nursing care process of a patient who was diagnosed with cervical disc herniation was examined according to Neuman’s Systems Model. Person-specific, interpersonal and extrinsic stressors were identified in the direction of the obtained data, and nursing diagnoses suitable for the patient were established in the direction of stressors. As a result, it was found that the use of the model in this case was appropriate and it was suggested to be applied in other patient groups to evaluate the effectiveness of the model. Extended English summary is in the end of Full Text PDF (TURKISH) file.ÖzetHemşirelik profesyonel bir sağlık disiplinidir ve bilimsel bilgi kaynağını kavram,[Mh1] kuram ve modellerden almaktadır. Neuman Sistemler Modeli bu modellerden birisidir. Model bireyin iyilik haline odaklanmakta ve bütüncül hemşirelik bakımını esas almaktadır. Modelde birey, algıladığı stresörleri yorumlayan açık sistemdir. Hemşire bu stresörleri tanımlayarak bireyin savunma mekanizmalarını birincil, ikincil ve üçüncül düzeyde geliştirecek bakımın planlanması, uygulanması ve değerlendirilmesinden sorumludur. Günlük yaşam aktivitelerini olumsuz etkileyen servikal disk herni tanısı alan bireyin algıladığı stresörlerle başa çıkmasına yardım edilerek bireyin yaşam kalitesi artırılabilir, tedavi süreci kısaltılabilir ve maliyet azaltılabilir. Bu çalışmada servikal disk hernisi tanısı alan bir hastanın hemşirelik bakım süreci Neuman Sistemler modeline göre incelenmiştir. Elde edilen veriler doğrultusunda kişiye özgü, kişiler arası ve kişi dışı stresörler belirlenmiş, stresörler doğrultusunda hastaya uygun hemşirelik tanıları konulmuştur. Sonuç olarak, modelin bu olguda kullanımının uygun olduğu görülmüş, modelin etkinliğini değerlendirmek amacıyla başka hasta gruplarında da uygulanması önerilmiştir.