bluuebunny/crossref_metadata_2025_split huggingface dataset:
APC/Cdh1 targets PECAM‐1 for ubiquitination and degradation in endothelial cells
Authors: Liu Jia , Yao Qinyu , Xiao Lei , Li Fan , Ma Wen , Zhang Zihui , Xie Xinya , Yang Chunmiao , Cui Qi , Tian Ying , Zhang Chao , Lai Baochang , Wang Nanping 
Published: September 2019

AbstractPlatelet endothelial cell adhesion molecule‐1 (PECAM‐1) is a member of the immunoglobulin superfamily and is expressed by hematopoietic and endothelial cells (ECs). Recent studies have shown that PECAM‐1 plays a crucial role in promoting the development of the EC inflammatory response in the context of disturbed flow. However, the mechanistic pathways that control PECAM‐1 protein stability remain largely unclear. Here, we identified PECAM‐1 as a novel substrate of the APC/Cdh1 E3 ubiquitin ligase. Specifically, lentivirus‐mediated Cdh1 depletion stabilized PECAM‐1 in ECs. Conversely, overexpression of Cdh1 destabilized PECAM‐1. The proteasome inhibitor MG132 blocked Cdh1‐mediated PECAM‐1 degradation. In addition, Cdh1 promoted K48‐linked polyubiquitination of PECAM‐1 in a destruction box‐dependent manner. Furthermore, we demonstrated that compared with pulsatile shear stress (PS), oscillatory shear stress decreased the expression of Cdh1 and the ubiquitination of PECAM‐1, therefore stabilizing PECAM‐1 to promote inflammation in ECs. Hence, our study revealed a novel mechanism by which fluid flow patterns regulate EC homeostasis via Cdh1‐dependent ubiquitination and subsequent degradation of PECAM‐1.
A large‐scale transcriptome analysis identified ELANE and PRTN3 as novel methylation prognostic signatures for clear cell renal cell carcinoma
Authors: Wei Zhongheng , Wu Bo , Wang Li , Zhang Juan 
Published: September 2019

AbstractDNA methylation was involved in the progress of many types of cancer including clear cell renal cell carcinomas (ccRCCs). This study aimed to identify the prognostic DNA methylation biomarkers for the ccRCCs by a large‐scale RNA‐seq analysis. The DNA methylation data and the corresponding clinical information of the patients with ccRCCs were extracted from TCGA database and randomly divided into the training group and the validation group. The differentially expressed CpG sites and the survival‐related CpG sites were further identified, which was combined into CpG sites pair and followed by screening the survival‐related pairs. The C‐index and the forward search algorithms were constructed to identify the prognostic signatures for the patients with ccRCCs. The prognostic signatures were verified by the validation dataset and the protein–protein interactions (PPI) network analysis was performed on the CPG sites of the signature. A total of 9,861 differentially expressed CPG sites were identified and 567 CpG sites were found to relate to the overall survival (OS) of the patients with ccRCCs. Besides, 1,146 CPG sites pairs were found to be related to the OS of the ccRCCs samples and the signature composed of seven CpG sites pairs were obtained to predict the prognosis of patients with ccRCCs and the results were verified in the validation dataset. Besides, the PPI network analysis showed that ELANE and PRTN3 gene may be associated with the invasion and metastasis of ccRCCs and could function as potential prognostic and therapeutic signatures for ccRCCs.
A new method for locating Kikuchi bands in electron backscatter diffraction patterns
Authors: Zhang Yongsheng , Fang Shangqiang , Lin Chucheng , Zhang Jimei , Jiang Caifen , Zeng Yi , Huang Fuqiang , Miao Hong 
Published: September 2019

AbstractElectron backscatter diffraction (EBSD) device can provide crystal structure, orientation, and phase content data through analysis of EBSD patterns. The reliability and precision of these data depend on the quality of the band position and zone axes data. This study introduces a new image processing method that can accurately provide the location of Kikuchi bands and poles. In this method, pattern rotation and gray gradient calculation are employed after for the initial detection of Kikuchi lines. Hough transform and Gaussian function are used for the final definition of bands position. Based on the position of Kikuchi bands, the indices of lattice planes and zone axes can be obtained precisely and easily. Angles between zone axes are calculated using locating results. The maximum error for a single‐crystal silicon sample is only 8.07%, illustrating the accuracy of this new method.
Compartmental function and modulation of the striatum
Authors: Prager Eric M. , Plotkin Joshua L. 
Published: September 2019

AbstractThe striatum plays a central role in guiding numerous complex behaviors, ranging from motor control to action selection and reward learning. The diverse responsibilities of the striatum are reflected by the complexity of its organization. In this review, we will summarize what is currently known about the compartmental layout of the striatum, an organizational principle that is crucial for allowing the striatum to guide such a diverse array of behaviors. We will focus on the anatomical and functional properties of striosome (patch) and matrix compartments of the striatum, and how the engagement of these compartments is uniquely controlled by their afferents, intrinsic properties, and neuromodulation. We will give examples of how advances in technology have opened the door to functionally dissecting the striatum's compartmental design, and close by offering thoughts on the future and relevance for human disease.
Single‐Fiber Laser Ablation in Treating Selected Metastatic Lymph Nodes of Papillary Thyroid Carcinoma and Benign Cold Thyroid Nodules—Preliminary Results
Authors: Guo YuQing , Li ZhiXian , Wang SiDa , Liao XianShan , Li Chen 
Published: September 2019

Background and ObjectivesTo evaluate the feasibility and efficacy of single‐fiber laser ablation (LA) under ultrasound guidance and appropriate ablation modes in the treatment of selected metastatic lymph nodes of papillary thyroid carcinoma (PTC) and benign cold thyroid nodulesStudy Design/Materials and MethodsA total of 18 patients (consisting of 8 patients with 18 metastatic lymph nodes of PTC and 10 patients with 10 benign cold thyroid nodules) each underwent one session of single‐fiber LA under ultrasound guidance. On the basis of the sizes of the nodules, the ablation modes were chosen accordingly. The single‐dot ablation mode was used in the nodules with three orthogonal diameters measuring no greater than 10 mm in diameter, with a dot, a level and an insertion. The double‐dots overlapping ablation mode was used in the nodules with the largest diameters measuring greater than 10 mm (in which the nodules measured no more than 15 mm in diameter and with the other two perpendicular diameters measuring no greater than 10 mm in diameter) with two dots, a level and two insertions. The multiple levels and dots overlapping ablation mode was used in the nodules with the three orthogonal diameters all measuring larger than 10 mm, with multiple dots, levels and insertions.ResultsAfter 12 months of follow‐up in the treated nodules of the metastatic lymph nodes of PTC and benign cold thyroid nodules, the mean baseline volumes decreased from 0.29 ± 0.12  to 0.03 ± 0.03 ml and 3.85 ± 0.64 to 1.1 ± 0.37 ml, respectively, and the mean volume reduction ratios (VRRs), which was calculated as {[(initial volume−final volume) × 100%]/initial volume}, were 90.3 ± 7.6% and 72 ± 5.8%, respectively. There were six ablative zones that completely disappeared, whereas the ablative zones that still existed presented as scar‐like areas or small hyperechoic areas that were compatible with scar tissue among the 18 malignant nodules at the last follow‐up.ConclusionsSingle‐fiber LA under ultrasound guidance, with the appropriate ablation modes, is feasible and effective for the treatment of selected metastatic lymph nodes of PTC and benign cold thyroid nodules. This study suggests that single‐fiber LA may be applied to selected cases with appropriate ablation modes. Lasers Surg. Med. 2019 © 2019 Wiley Periodicals, Inc.
Covalently Binding of Bovine Serum Albumin to Unsaturated Poly(Globalide‐Co‐ε‐Caprolactone) Nanoparticles by Thiol‐Ene Reactions
Authors: Guindani Camila , Frey Marie‐Luise , Simon Johanna , Koynov Kaloian , Schultze Jennifer , Ferreira Sandra R. S. , Araújo Pedro H. H. , de Oliveira Débora , Wurm Frederik R. , Mailänder Volker , Landfester Katharina 
Published: September 2019

AbstractWhen nanoparticles (NPs) are introduced to a biological fluid, different proteins (and other biomolecules) rapidly get adsorbed onto their surface, forming a protein corona capable of giving to the NPs a new “identity” and determine their biological fate. Protein–nanoparticle conjugation can be used in order to promote specific interactions between living systems and nanocarriers. Non‐covalent conjugates are less stable and more susceptible to desorption in biological media, which makes the development of engineered nanoparticle surfaces by covalent attachment an interesting topic. In this work, the surface of poly(globalide‐co‐ε‐caprolactone) (PGlCL) nanoparticles containing double bonds in the main polymer chain is covalently functionalized with bovine serum albumin (BSA) by thiol‐ene chemistry, producing conjugates which are resistant to dissociation. The successful formation of the covalent conjugates is confirmed by flow cytometry (FC) and fluorescence correlation spectroscopy (FCS). Transmission electron microscopy (TEM) allows the visualization of the conjugate formation, and the presence of a protein layer surrounding the NPs can be observed. After conjugation with BSA, NPs present reduced cell uptake by HeLa and macrophage RAW264.7 cells, in comparison to uncoated NP. These results demonstrate that it is possible to produce stable conjugates by covalently binding BSA to PGlCL NP through thiol‐ene reaction.
Bio‐Based Nanofibers Involved in Wastewater Treatment
Authors: Gandavadi Dhanush , Sundarrajan Subramanian , Ramakrishna Seeram 
Published: September 2019

AbstractStudies involving bio‐based nanofibers are well utilized in the field of energy, catalysis, electronics, and environmental science. In this review, the importance of utilizing bio‐based materials for the development and optimization of multiplexed nanofibers and the modifications adopted to overcome the drawbacks of conventional electro‐spinning to facilitate better production rates, enhanced adsorption, and its potential in removing heavy metal ions, dyes, and other contaminants polluting the environment are highlighted. This work provides the readers the ability to understand the complexity in fabrication of bio‐based nanofibers focusing primarily on chitosan, cellulose, and protein‐based nanofibers and their mechanism toward quenching/degradation of water contaminants. In addition, it also provides the advantages of using bio‐based materials over synthetic materials for the development of nanofibers.
Phase Transition Behaviors and Nanoscale Film Morphologies of Poly(δ‐valerolactone) Axles Bearing Movable and Fixed Rotaxane Wheels
Authors: Ree Brian J. , Aoki Daisuke , Kim Jehan , Satoh Toshifumi , Takata Toshikazu , Ree Moonhor 
Published: September 2019

AbstractIn this study, poly(δ‐valerolactone) (PVL) axles bearing movable and fixed dibenzo‐24‐crown‐8‐ether wheels (rot‐M and rot‐F) are investigated for the first time in the terms of phase transition and nanoscale film morphology: PVL‐rot‐M and PVL‐rot‐F. Interestingly, the PVL axles reveal a strong tendency to form a horizontal lamellar structure with three different rotational crystal lattice domains in nanoscale films. The morphological structural parameters are discernibly varied by the movable and fixed rotaxane wheels. In particular, the rot‐M wheel tends to be populated in both the interfacial and amorphous layers. The rot‐M wheel is found to significantly influence the phase transition characteristics of the PVL axle because of its movability along the polymer backbone chain. In contrast, the rot‐F wheel tends to be more localized in the interfacial layer rather than in the amorphous layer because of its immovability constrained at the polymer chain end. The rot‐F wheel causes severe thermal instability in the PVL axle, which can be attributed mainly to the presence of its counter anion (PF6−).
Impact of four lncRNA polymorphisms (rs2151280, rs7763881, rs1136410, and rs3787016) on glioma risk and prognosis: A case‐control study
Authors: Deng Yujiao , Zhou Linghui , Li Na , Wang Meng , Yao Li , Dong Shanshan , Zhang Ming , Yang Pengtao , Hao Qian , Wu Ying , Lyu Lijuan , Jin Tianbo , Dai Zhijun , Kang Huafeng 
Published: September 2019

AbstractLong noncoding RNA (lncRNA) polymorphisms are reportedly in connection with tumor susceptibility and prognosis. Glioma is one of the most aggressive and common cancers of the central nervous system. This study aimed to investigate the relationship between four lncRNA variants and glioma susceptibility and prognosis in a Chinese Han population. Sequenom Mass‐ARRAY was used to genotype 605 patients with glioma and 1300 cancer‐free individuals. Odds ratios or hazard ratios and related 95% confidence intervals were calculated to estimate the correlations. Logistic and Cox regression models, log‐rank tests, and Kaplan‐Meier curves were used for the statistical analysis. Six inheritance models showed that ANRIL rs2151280 variant genotype (A>G) was related to the susceptibility of glioma, while the other three lncRNAs showed no association. Patients treated with temozolomide or nimustine had better progression‐free survival (PFS) and overall survival (OS) than those treated with platinum. Besides, patients aged older than 40 years showed a poorer OS. The Cox multivariate analysis revealed that the rs1136410 GG genotype (A>G) was beneficial for OS and PFS. The Kaplan‐Meier analyses indicated that rs1136410 A>G and the rs7763881 A>C were associated with longer OS. ANRIL rs2151280 variant genotype might increase susceptibility of glioma. In addition, PARP1 rs1136410 variant genotype could be beneficial for the overall survival of patients with glioma. More research data are needed to further validate our results.
Sphingosine‐1‐phosphate (S1P) analog phytosphingosine‐1‐phosphate (P1P) improves the in vitro maturation efficiency of porcine oocytes via regulation of oxidative stress and apoptosis
Authors: Park Kyu‐Mi , Wang Jae Woong , Yoo Yeong‐Min , Choi Myeong Jun , Hwang Kyu Chan , Jeung Eui‐Bae , Jeong Yeon Woo , Hwang Woo Suk 
Published: September 2019

AbstractPhytosphingosine‐1‐phosphate (P1P) is a signaling sphingolipid that regulates various physiological activities. However, little is known about the effect of P1P in the context of reproduction. Thus, we aimed to investigate the influence of P1P on oocyte maturation during porcine in vitro maturation (IVM). Here, we report the expression of S1PR1–3 among P1P receptors (S1PR1–4) in cumulus cells and oocytes. When P1P was administered at concentrations of 10, 50, 100, and 1,000 nM during IVM, the metaphase II rate was significantly increased in the 1,000 nM (1 μM) P1P treatment group. Maturation rate improvement by P1P supplementation was observed only in the presence of epidermal growth factor (EGF). Oocytes under the influence of P1P showed decreased intracellular reactive oxygen species levels but no significant differences in glutathione levels. In our molecular studies, P1P treatment upregulated gene expression involved in cumulus expansion (Has2 and EGF), antioxidant enzymes (SOD3 and Cat), and developmental competence (Oct4) while activating extracellular signal‐regulated kinase1/2 and Akt signaling. P1P treatment also influenced oocyte survival by shifting the ratio of Bcl‐2 to Bax while inactivating JNK signaling. We further demonstrated that oocytes matured with P1P displayed significantly higher developmental competence (cleavage and blastocyst [BL] formation rate) and greater BL quality (total cell number and the ratio of apoptotic cells) when activated via parthenogenetic activation (PA) and in vitro fertilization. Despite the low levels of endogenous P1P found in animals, exogenous P1P influenced animal reproduction, as shown by increased porcine oocyte maturation as well as preimplantation embryo development. This study and its findings are potentially relevant for both human and animal‐assisted reproduction.
Cell‐based maximum entropy approximants for three‐dimensional domains: Application in large strain elastodynamics using the meshless total Lagrangian explicit dynamics method
Authors: Mountris Konstantinos A. , Bourantas George C. , Millán Daniel , Joldes Grand R. , Miller Karol , Pueyo Esther , Wittek Adam 
Published: September 2019

SummaryWe present the cell‐based maximum entropy (CME) approximants in E3 space by constructing the smooth approximation distance function to polyhedral surfaces. CME is a meshfree approximation method combining the properties of the maximum entropy approximants and the compact support of element‐based interpolants. The method is evaluated in problems of large strain elastodynamics for three‐dimensional (3D) continua using the well‐established meshless total Lagrangian explicit dynamics method. The accuracy and efficiency of the method is assessed in several numerical examples in terms of computational time, accuracy in boundary conditions imposition, and strain energy density error. Due to the smoothness of CME basis functions, the numerical stability in explicit time integration is preserved for large time step. The challenging task of essential boundary condition (EBC) imposition in noninterpolating meshless methods (eg, moving least squares) is eliminated in CME due to the weak Kronecker‐delta property. The EBCs are imposed directly, similar to the finite element method. CME is proven a valuable alternative to other meshless and element‐based methods for large‐scale elastodynamics in 3D. A naive implementation of the CME approximants in E3 is available to download at https://www.mountris.org/software/mlab/cme.
Restitution volumes at the end of dialysis sessions: A potential influencing factor on patients' haemoglobin levels?
Authors: Matos Joao Fazendeiro , Peralta Ricardo , Felix Carla , Pinto Bruno , Goncalves Pedro , Carlos Vera , Rodrigues Ricardo , Parisotto Maria Teresa , Carvalho Maria João , Ponce Pedro 
Published: September 2019

AbstractAimTo evaluate whether haemoglobin (Hb) levels are influenced by the restitution volume (RestVol) at the end of the dialysis session, independently of erythropoiesis‐stimulating agents (ESA) and iron doses.DesignOver 12 months, 4,386 haemodialysis patients from 34 centres were enrolled in this observational descriptive study according to the checklist STrengthening the Reporting of Observational Studies in Epidemiology (STROBE).MethodRestVol, Hb levels, ESA and iron doses of every patient were assessed on a monthly basis. To determine the ideal RestVol, the clinics were classified into three groups according to the restitution volumes at the end of the dialysis sessions.ResultsMean age was 69 ± 14 years, and 58.9% were men. The evaluation of 665,712 treatments revealed that RestVol of 380 ml seems to be the most efficient, since the clinics in this group managed to reduce ESA consumption with a negligible reduction in Hb levels.
Crystallization rates of moderate and ultrahigh molecular weight polyethylene characterized by Flash DSC measurement
Authors: Lv Ruihua , He Yucheng , Xie Kefeng , Hu Wenbing 
Published: September 2019

AbstractWe compared crystallization rates in three bulk commercial polyethylene samples separately consisting of two ultrahigh molecular weights and one moderate molecular weight. The lower‐end temperature of isothermal crystallization was expanded by means of Flash DSC measurement in order to approach the practically fast crystallization region. The results showed that, in the whole temperature range of our observations, ultrahigh molecular weights make polyethylene crystallization faster than moderate molecular weights. This behaviour is opposite to the general trend in linear polymers. In addition, ultrahigh molecular weights make polyethylene crystals resist fast heating before melting more than moderate molecular weights, due to high entanglement around the crystals. We discuss the possible mechanism of fast crystallization in ultrahigh molecular weight polyethylene, which is beneficial for prosthesis uses. © 2019 Society of Chemical Industry
Lipid Atlas of Keratinocytes and Betulin Effects on its Lipidome Profiled by Comprehensive UHPLC–MS/MS with Data Independent Acquisition Using Targeted Data Processing
Authors: Calderón Carlos , Rubarth Lara , Cebo Malgorzata , Merfort Irmgard , Lämmerhofer Michael 
Published: September 2019

AbstractBetulin is a pentacyclic triterpene with demonstrated healing properties in mid‐dermal wounds. A few earlier studies have provided insights into the wound healing effects on the molecular level. However, there are still questions left on the molecular targets of betulin. Therefore, a pharmacolipidomics analysis of betulin is undertaken in human immortalized keratinocytes to monitor alterations in the lipid profiles induced by treatment with betulin. For this purpose, lipid extracts of keratinocytes treated with betulin and untreated controls are comprehensively analyzed by an untargeted UHPLC–ESI–QTOF‐MS/MS lipidomics profiling workflow using data‐independent acquisition. Targeted data processing allows the identification of 611 lipid species from 21 different lipid classes. Statistical analysis of the identified lipids shows significant changes in 440 lipid species that can be described as downregulation of cholesteryl esters and triacylglycerides and upregulation of glycerophospholipids, sphingolipids, and diacylglycerides. Additionally, some other signals corresponding to triterpenes are found in the betulin group and suggested that betulin is incorporated (in the membrane) and metabolized in keratinocytes.