bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surpassing the single-atom catalytic activity limit through paired Pt-O-Pt ensemble built from isolated Pt1 atoms
Authors: Wang Hui , Liu Jin-Xun , Allard Lawrence F. , Lee Sungsik , Liu Jilei , Li Hang , Wang Jianqiang , Wang Jun , Oh Se H. , Li Wei , Flytzani-Stephanopoulos Maria , Shen Meiqing , Goldsmith Bryan R. , Yang Ming 
Published: August 2019

AbstractDespite the maximized metal dispersion offered by single-atom catalysts, further improvement of intrinsic activity can be hindered by the lack of neighboring metal atoms in these systems. Here we report the use of isolated Pt1 atoms on ceria as “seeds” to develop a Pt-O-Pt ensemble, which is well-represented by a Pt8O14 model cluster that retains 100% metal dispersion. The Pt atom in the ensemble is 100–1000 times more active than their single-atom Pt1/CeO2 parent in catalyzing the low-temperature CO oxidation under oxygen-rich conditions. Rather than the Pt-O-Ce interfacial catalysis, the stable catalytic unit is the Pt-O-Pt site itself without participation of oxygen from the 10–30 nm-size ceria support. Similar Pt-O-Pt sites can be built on various ceria and even alumina, distinguishable by facile activation of oxygen through the paired Pt-O-Pt atoms. Extending this design to other reaction systems is a likely outcome of the findings reported here.
A co-expressed gene status of adenylate kinase 1/4 reveals prognostic gene signature associated with prognosis and sensitivity to EGFR targeted therapy in lung adenocarcinoma
Authors: Jan Yi-Hua , Lai Tsung-Ching , Yang Chih-Jen , Huang Ming-Shyan , Hsiao Michael 
Published: August 2019

AbstractCancer cells utilize altered bioenergetics to fuel uncontrolled proliferation and progression. At the core of bioenergetics, adenine nucleotides are the building blocks for nucleotide synthesis, energy transfer and diverse metabolic processes. Adenylate kinases (AK) are ubiquitous phosphotransferases that catalyze the conversion of adenine nucleotides and regulate the homeostasis of nucleotide ratios within cellular compartments. Recently, different isoforms of AK have been shown to induce metabolic reprograming in cancer and were identified as biomarkers for predicting disease progression. Here we aim to systemically analyze the impact of all AK-associated gene signatures on lung adenocarcinoma patient survival and decipher the value for therapeutic interventions. By analyzing TCGA Lung Adenocarcinoma (LUAD) RNA Seq data, we found gene signatures from AK4 and AK1 have higher percentage of prognostic genes compared to other AK-gene signatures. A 118-gene signature was identified from consensus gene expression in AK1 and AK4 prognostic gene signatures. Immunohistochemistry (IHC) analyses in 140 lung adenocarcinoma patients showed overexpression of AK4 significantly correlated with worse overall survival (P = 0.001) whereas overexpression of AK1 significantly associated with good prognosis (P = 0.009). Furthermore, reduced AK4 expression by shRNA reduced the EGFR protein expression in EGFR mutation cells. The inhibition of AK4-AK1 signal might provide a potential target for synergistic effect in target therapy in lung cancer patients.
An Observed Regime Shift in the Formation of Warm Core Rings from the Gulf Stream
Authors: Gangopadhyay Avijit , Gawarkiewicz Glen , Silva E. Nishchitha S. , Monim M. , Clark Jenifer 
Published: August 2019

AbstractWe present observational evidence that a significant regime change occurred around the year 2000 in the formation of Warm Core Rings (WCRs) from the Gulf Stream (GS) between 75° and 55°W. The dataset for this study is a set of synoptic oceanographic charts available over the thirty-eight-year period of 1980–2017. The upward regime change shows an increase to 33 WCRs per year during 2000–2017 from an average of 18 WCRs during 1980 to 1999. A seasonal analysis confirms May-June-July as the peak time for WCR births in agreement with earlier studies. The westernmost region (75°-70°W) is least ring-productive, while the region from 65°W to 60°W is most productive. This regime shift around 2000 is detected in WCR formation for all of the four 5-degree wide sub-regions and the whole region (75°-55°W). This might be related to a reduction of the deformation radius for ring formation, allowing unstable meanders to shed more frequent rings in recent years. A number of possible factors resulting in such a regime shift related to the possible changes in reduced gravity, instability, transport of the GS, large-scale changes in the wind system and atmospheric fluxes are outlined, which suggest new research directions. The increase in WCRs has likely had an impact on the marine ecosystem since 2000, a topic worthy for future studies.
Agent-based simulations of China inbound tourism network
Authors: Wu Jinfeng , Wang Xingang , Pan Bing 
Published: August 2019

AbstractBased on the results of a large-scale survey, we construct an agent-based network model for the China independent inbound tourism system and, by the approach of numerical simulation, investigate the responses of the tourist behaviors to perturbations in different scenarios, including the closure of a tourism city, the opening of a new port city in western China, and the increase of the tourism attractiveness of a specific city. Numerical results show that: (1) the closure of a single city in general will affect the tourist visitations of many other cities and, comparing to the non-port cities, the overall visitation volume of the system is more influenced by closing a port city; (2) the opening of a new port city in western China will attract more tourists to the western cities, but has a negligible impact on either the overall visitation volume or the imbalanced tourist distribution; and (3) the increase of the tourism attractiveness of a non-port (port) city normally increases (decreases) the overall visitation volume, yet there are exceptions due to the spillover effect. Furthermore, by increasing the tourism attractiveness of a few cities simultaneously, we investigate also the strategy of multiple-city-upgrade in tourism development. We find that the overall tourist volume is better improved by upgrading important non-port cities that are geographically distant from each other. The study reveals the rich dynamic inherent in complex tourism network, and the findings could be helpful to the development and management of China inbound tourism.
Disruptive effects of repeated stress on basolateral amygdala neurons and fear behavior across the estrous cycle in rats
Authors: Blume Shannon R. , Padival Mallika , Urban Janice H. , Rosenkranz J. Amiel 
Published: August 2019

AbstractStress is a precipitating factor in depression and anxiety disorders. Patients with these disorders often show amygdala abnormalities. The basolateral amygdala (BLA) is integral in mood and emotion, and is sensitive to stress. While much is known about effects of stress on BLA neuron activity and morphology in males, less is known in females. We tested whether repeated stress exerts distinct effects on BLA in vivo neuronal activity and morphology of Golgi-stained BLA neurons [lateral (LAT) and basal (BA) nuclei] in adult female rats. Repeated restraint stress increased BLA neuronal firing and caused hypertrophy of BLA neurons in males, while it decreased LAT and BA neuronal firing and caused hypotrophy of neurons in the LAT of females. BLA neuronal activity and function, such as fear conditioning, shifts across the estrous cycle. Repeated stress disrupted this pattern of BLA activity and fear expression over the estrous cycle. The disruptive effects of stress on the pattern of BLA function across estrous may produce behavior that is non-optimal for a specific phase of the estrous cycle. The contrasting effects of stress may contribute to sex differences in the effects of stress on mood and psychiatric disorders.
Deaf readers benefit from lexical feedback during orthographic processing
Authors: Gutierrez-Sigut Eva , Vergara-Martínez Marta , Perea Manuel 
Published: August 2019

AbstractIt has been proposed that poor reading abilities in deaf readers might be related to weak connections between the orthographic and lexical-semantic levels of processing. Here we used event related potentials (ERPs), known for their excellent time resolution, to examine whether lexical feedback modulates early orthographic processing. Twenty congenitally deaf readers made lexical decisions to target words and pseudowords. Each of those target stimuli could be preceded by a briefly presented matched-case or mismatched-case identity prime (e.g., ALTAR-ALTAR vs. altar- ALTAR). Results showed an early effect of case overlap at the N/P150 for all targets. Critically, this effect disappeared for words but not for pseudowords, at the N250—an ERP component sensitive to orthographic processing. This dissociation in the effect of case for word and pseudowords targets provides strong evidence of early automatic lexical-semantic feedback modulating orthographic processing in deaf readers. Interestingly, despite the dissociation found in the ERP data, behavioural responses to words still benefited from the physical overlap between prime and target, particularly in less skilled readers and those with less experience with words. Overall, our results support the idea that skilled deaf readers have a stronger connection between the orthographic and the lexical-semantic levels of processing.
Compact all-fiber source of coherent linearly polarized octave-spanning supercontinuum based on normal dispersion silica fiber
Authors: Tarnowski Karol , Martynkien Tadeusz , Mergo Paweł , Sotor Jarosław , Soboń Grzegorz 
Published: August 2019

AbstractWe report the generation of coherent octave-spanning supercontinuum in an all-fiber system, without any free-space optical components. The setup uses the femtosecond fiber laser as a pump and an all-normal dispersion microstructured fiber as a medium for supercontinuum generation. The generated spectrum is characterized both experimentally and numerically and shows a broad bandwidth (1.1−2.2 μm), a high signal to noise ratio reaching 100 at maximum, a high coherence (closing to 1), linear polarization and average output power up to 57 mW. The source is characterized by exceptional simplicity and does not require any alignment (the nonlinear fiber is spliced to the pump) which finally opens the path to outside-lab applications of supercontinuum radiation.
Sex and age specific reduction in stress resistance and mitochondrial DNA copy number in Drosophila melanogaster
Authors: Kristensen Torsten Nygaard , Loeschcke Volker , Tan Qihua , Pertoldi Cino , Mengel-From Jonas 
Published: August 2019

AbstractEnvironmental stresses such as extreme temperatures, dehydration and food deprivation may have distinct consequences for different age-classes and for males and females across species. Here we investigate a natural population of the model organism Drosophila melanogaster. Males and females at ages 3, 19 and 35 days were tested for stress resistance; i.e. the ability of flies to cope with starvation and both cold and hot temperatures. Further, we tested a measure of metabolic efficiency, namely mitochondrial DNA copy number (mtDNA CN) in both sexes at all three age-classes. We hypothesize that stress resistance is reduced at old age and more so in males, and that mtDNA CN is a biomarker for sex- and age-dependent reductions in the ability to cope with harsh environments. We show that: (1) males exhibit reduced starvation tolerance at old age, whereas older females are better in coping with periods without food compared to younger females, (2) heat tolerance decreases with increasing age in males but not in females, (3) cold tolerance is reduced at old age in both sexes, and (4) old males have reduced mtDNA CN whereas mtDNA CN slightly increases with age in females. In conclusion, our data provide strong evidence for trait and sex specific consequences of aging with females generally being better at coping with environmental stress at old age. The reduced mtDNA CN in old males suggests reduced metabolic efficiency and this may partly explain why males are less stress tolerant at old age than females. We suggest that mtDNA CN might be a suitable biomarker for physiological robustness. Our findings likely extend to other taxa than Drosophila and therefore we discuss the observations in relation to aging and sex specific lifespan across species.
Association of MTMR3 rs12537 at miR-181a binding site with rheumatoid arthritis and systemic lupus erythematosus risk in Egyptian patients
Authors: Senousy Mahmoud A. , Helmy Hebatullah S. , Fathy Nevine , Shaker Olfat G. , Ayeldeen Ghada M. 
Published: August 2019

AbstractSingle nucleotide polymorphisms (SNPs) in microRNA-target sites influence an individual’s risk and prognosis for autoimmune diseases. Myotubularin-related protein 3 (MTMR3), an autophagy-related gene, is a direct target of miR-181a. We investigated whether MTMR3 SNP rs12537 in the miR-181a-binding site is associated with the susceptibility and progression of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Overall, 94 patients with RA, 80 patients with SLE, and 104 healthy volunteers were recruited. Genotyping and expression analysis of circulating MTMR3 and miR-181a were performed by qPCR. The autophagic marker MAP1LC3B was measured by ELISA. The rs12537 minor homozygote (TT) genotype was a candidate risk factor of both RA and SLE. rs12537TT was associated with lower serum MTMR3 expression and higher LC3B levels than other genotypes in patients with both diseases. Serum miR-181a expression was higher in rs12537TT carriers than in other genotypes among SLE patients. Serum miR-181a and MTMR3 levels were inversely correlated in SLE but not in RA patients. rs12537TT and serum miR-181a were positively associated with disease severity in both diseases. Our results identify a novel role of rs12537 in the susceptibility and progression of RA and SLE, possibly through impacting the interaction between miR-181a and MTMR3 leading to increased autophagy.
InN superconducting phase transition
Authors: Song Zhi-Yong , Shang Liyan , Hu Zhigao , Chu JunHao , Chen Ping-Ping , Yamamoto Akio , Kang Ting-Ting 
Published: August 2019

AbstractInN superconductivity is very special among III–V semiconductors, as other III–V semiconductors (such as GaAs, GaN, InP, InAs, etc.) usually lack strong covalent bonding and thus seldom show superconductivity at low temperatures. Here, we probe the different superconducting phase transitions in InN highlighted by its microstructure. Those chemical-unstable phase-separated inclusions, such as metallic indium or In2O3, are intentionally removed by HCl acid etching. The quasi-two-dimensional vortex liquid-glass transition is observed in the sample with a large InN grain size. In contrast, the superconducting properties of InN with a small grain size are sensitive to acid etching, showing a transition into a nonzero resistance state when the temperature approaches zero. Since the value of ξ0 (the zero-temperature-limit superconducting coherence length) is close to the grain size, it is suggested that individual InN grains and intergrain coupling should be responsible for the sample-dependent InN superconducting phase transition. Our work establishes a guideline for engineering superconductivity in III-nitride.
Constitutive PGC-1α overexpression in skeletal muscle does not protect from age-dependent decline in neurogenesis
Authors: Karlsson Lars , González-Alvarado María Nazareth , Motalleb Reza , Blomgren Klas , Börjesson Mats , Kuhn Hans Georg 
Published: August 2019

AbstractAerobic exercise prevents age-dependent decline in cognition and hippocampal neurogenesis. The transcription factor peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC-1α) mediates many of the exercise-induced benefits in skeletal muscle, including the release of factors into the circulation with neurotrophic effects. We use a transgenic mouse model with muscle-specific overexpression of PGC-1α to study the contribution of chronic muscle activation on exercise-induced effects on hippocampal neurogenesis in aging. Young and old transgenic and wild type animals of both sexes displayed a robust age-related reduction in newborn BrdU+-cells, immature neurons (DCX+-cells) and new mature BrdU+/NeuN+-neurons in the dentate gyrus. No differences were detected between genotypes or sexes. Analysis of serum proteins showed a tendency towards increased levels of myokines and reduced levels of pro-inflammatory cytokines for transgenic animals, but only musclin was found to be significantly up-regulated in transgenic animals. We conclude that constitutive muscular overexpression of PGC-1α, despite potent systemic changes, is insufficient for mimicking exercise-induced effects on hippocampal neurogenesis in aging. Continued studies are required to investigate the complex molecular mechanisms by which circulating signals could mediate exercise-induced effects on the central nervous system in disease and aging, with the aim of discovering new therapeutic possibilities for patients.
+50 Years of Terrestrial Hydroclimatic Variability in Africa’s Transboundary Waters
Authors: Hasan Emad , Tarhule Aondover , Zume Joseph T. , Kirstetter Pierre-Emmanuel 
Published: August 2019

AbstractGRACE Terrestrial Water Storage (TWS) provides unique and unprecedented perspectives about freshwater availability and change globally. However, GRACE-TWS records are relatively short for long-term hydroclimatic variability studies, dating back to April 2002. In this paper, we made use of Noah Land Surface Model (LSM), and El Niño–Southern Oscillation (ENSO) data in an autoregressive model with exogenous variables (ARX) to reconstruct a 66-year record of TWS for nine major transboundary river basins (TRBs) in Africa. Model performance was evaluated using standard indicators, including the Nash Sutcliffe Efficiency criteria, cumulative density frequency, standardized residuals plots, and model uncertainty bounds. Temporally, the reconstruction results were evaluated for trend, cycles, and mode of variability against ancillary data from the WaterGAP Model (WGHM-TWS) and GPCC-based precipitation anomalies. The temporal pattern reveals good agreement between the reconstructed TWS, WGHM-TWS, and GPCC, (p-value < 0.0001). The reconstructed TWS suggests a significant declining trend across the northern and central TRBs since 1951, while the southern basins show an insignificant trend. The mode of variability analysis indicates short storage periodicity of four to sixteen-month in the northern basins, while strong intra-annual variability in the central and southern basins. The long-term TWS records provide additional support to Africa’s water resources research on hydroclimatic variability and change in shared transboundary water basins.
Structural features in the glycine-binding sites of the GluN1 and GluN3A subunits regulate the surface delivery of NMDA receptors
Authors: Skrenkova Kristyna , Hemelikova Katarina , Kolcheva Marharyta , Kortus Stepan , Kaniakova Martina , Krausova Barbora , Horak Martin 
Published: August 2019

AbstractN-methyl-D-aspartate receptors (NMDARs) are ionotropic glutamate receptors that play an essential role in mediating excitatory neurotransmission in the mammalian central nervous system (CNS). Functional NMDARs are tetramers composed of GluN1, GluN2A-D, and/or GluN3A-B subunits, giving rise to a wide variety of NMDAR subtypes with unique functional properties. Here, we examined the surface delivery and functional properties of NMDARs containing mutations in the glycine-binding sites in GluN1 and GluN3A subunits expressed in mammalian cell lines and primary rat hippocampal neurons. We found that the structural features of the glycine-binding sites in both GluN1 and GluN3A subunits are correlated with receptor forward trafficking to the cell surface. In addition, we found that a potentially clinically relevant mutation in the glycine-binding site of the human GluN3A subunit significantly reduces surface delivery of NMDARs. Taken together, these findings provide novel insight into how NMDARs are regulated by their glycine-binding sites and may provide important information regarding the role of NMDARs in both physiological and pathophysiological processes in the mammalian CNS.
TLR-2 mediated cytosolic-Ca2+ surge activates ER-stress-superoxide-NO signalosome augmenting TNF-α production leading to apoptosis of Mycobacterium smegmatis-infected fish macrophages
Authors: Hussain Md. Arafat , Datta Debika , Singh Rashmi , Kumar Manmohan , Kumar Jai , Mazumder Shibnath 
Published: August 2019

AbstractThe implications of TLR-2 mediated alterations in cytosolic-Ca2+((Ca2+)c) levels inM.smegmatisinfections is not well known. Using headkidney macrophages (HKM) fromClarias gariepinus, we observed TLR-2 signalling is required in the phagocytosis ofM.smegmatis.M.smegmatisinduced caspase-dependent HKM apoptosis in MOI, time and growth-phase dependent manner. RNAi and inhibitor studies demonstrated critical role of TLR-2 in eliciting (Ca2+)c-surge and c-Src-PI3K-PLC axis playing an intermediary role in the process. The (Ca2+)c-surge triggered downstream ER-stress and superoxide (O2−) generation. The cross-talk between ER-stress and O2−amplified TNF-α production, which led to HKM apoptosis and bacterial clearance. Release of nitric oxide (NO) was also observed and silencing the NOS2-NO axis enhanced intracellular bacterial survival and attenuated caspase activity. Pre-treatment with diphenyleneidonium chloride inhibited NO production implicating O2−–NO axis imperative inM.smegmatis-induced HKM apoptosis. NO positively impacted CHOP expression and TNF-α production in infected HKM. We conclude that, TLR-2 induced (Ca2+)c-surge and ensuing cross-talk between ER-stress and O2−potentiates HKM pathology by amplifying pro-inflammatory TNF-α production. Moreover, the pro-oxidant environment triggers NO release which prolonged ER-stress and TNF-α production, culminating in HKM apoptosis and bacterial clearance. Together, our study suggests HKM an alternate model to study macrophage-mycobacteria interactions.
Cell matrix adhesion in cell migration
Authors: Conway James R.W. , Jacquemet Guillaume 
Published: August 2019

Abstract
The ability of cells to migrate is a fundamental physiological process involved in embryonic development, tissue homeostasis, immune surveillance and wound healing. In order for cells to migrate, they must interact with their environment using adhesion receptors, such as integrins, and form specialized adhesion complexes that mediate responses to different extracellular cues. In this review, we discuss the role of integrin adhesion complexes (IACs) in cell migration, highlighting the layers of regulation that are involved, including intracellular signalling cascades, mechanosensing and reciprocal feedback to the extracellular environment. We also discuss the role of IACs in extracellular matrix remodeling and how they impact upon cell migration.