bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design and computational analysis of a novel Azurin-BR2 chimeric protein against breast cancer
Authors: Rehman Hafiz Muhammad , Yousaf Numan , Hina Syeda Mahlaqa , Nadeem Tariq , Ansari Mushtaq Ahmad , Chaudry Afeefa , Kafait Iram , Khalid Sania , Alanzi Abdullah R , Bashir Hamid 
Published: November 2024

Abstract
Cancer is one of most lethal diseases worldwide. Chemotherapeutics and surgeries are among the treatment facilities available for curing cancer. However due to their negative impact on normal cells and drug resistance development, new treatment strategies have yet to be developed. Some microbial products exhibit therapeutic potential for treating cancer. Pseudomonas aeruginosa Azurins have shown anticancer effects against breast cancer without affecting normal cells. To enhance its cytotoxic effect and targeted delivery, we fused Azurin with a cell-penetrating peptide (BR2) through a rigid linker and evaluated its anticancer potential via in silico analysis. The prediction of the secondary and the tertiary structures and analysis of physiochemical properties of chimeric proteins were computationally performed. The Azurin-BR2 chimeric protein has a basic nature with a molecular weight of 16.8 kDa. The quality indices and validation of chimeric proteins were performed with ERRAT2 and Ramachandran plot values, respectively. The quality index of the chimeric protein was predicted to be 81% to 84.6%, and residues residing in the most favoured region were identified. The HDOCK bioinformatics tool was used for docking a chimeric protein with a cancer suppressor protein p53. The results of the current study support that an Azurin-BR2 fusion protein has a high binding affinity for p53 can induce apoptosis in cancerous cells, and can be used in tumor-targeting therapy.
Bioinformatic workflows for deriving transcriptomic points of departure: current status, data gaps, and research priorities
Authors: O’Brien Jason , Mitchell Constance , Auerbach Scott , Doonan Liam , Ewald Jessica , Everett Logan , Faranda Adam , Johnson Kamin , Reardon Anthony , Rooney John , Shao Kan , Stainforth Robert , Wheeler Matthew , Dalmas Wilk Deidre , Williams Andrew , Yauk Carole , Costa Eduardo 
Published: November 2024

Abstract
There is a pressing need to increase the efficiency and reliability of toxicological safety assessment for protecting human health and the environment. Although conventional toxicology tests rely on measuring apical changes in vertebrate models, there is increasing interest in the use of molecular information from animal and in vitro studies to inform safety assessment. One promising and pragmatic application of molecular information involves the derivation of transcriptomic points of departure (tPODs). Transcriptomic analyses provide a snapshot of global molecular changes that reflect cellular responses to stressors and progression toward disease. A tPOD identifies the dose level below which a concerted change in gene expression is not expected in a biological system in response to a chemical. A common approach to derive such a tPOD consists of modeling the dose–response behavior for each gene independently and then aggregating the gene-level data into a single tPOD. Although different implementations of this approach are possible, as discussed in this manuscript, research strongly supports the overall idea that reference doses produced using tPODs are health protective. An advantage of this approach is that tPODs can be generated in shorter term studies (e.g. days) compared with apical endpoints from conventional tests (e.g. 90-d subchronic rodent tests). Moreover, research strongly supports the idea that reference doses produced using tPODs are health protective. Given the potential application of tPODs in regulatory toxicology testing, rigorous and reproducible wet and dry laboratory methodologies for their derivation are required. This review summarizes the current state of the science regarding the study design and bioinformatics workflows for tPOD derivation. We identify standards of practice and sources of variability in tPOD generation, data gaps, and areas of uncertainty. We provide recommendations for research to address barriers and promote adoption in regulatory decision making.
Transcriptomic Profiling of ‘Candidatus Liberibacter asiaticus’ in Different Citrus Tissues Reveals Novel Insights into Huanglongbing Pathogenesis
Authors: Lovelace Amelia H. , Wang Chunxia , Levy Amit , Ma Wenbo 
Published: November 2024

‘ Candidatus Liberibacter asiaticus’ (Las) is a gram-negative bacterial pathogen associated with citrus huanglongbing (HLB) or greening disease. Las is transmitted by the Asian citrus psyllid (ACP) where it colonizes the phloem tissue, resulting in substantial economic losses to the citrus industry worldwide. Despite extensive efforts, effective management strategies against HLB remain elusive, necessitating a deeper understanding of the pathogen's biology. Las undergoes cell-to-cell movement through phloem flow and colonizes different tissues in which Las may have varying interactions with the host. Here, we investigate the transcriptomic landscape of Las in citrus seed coat vasculatures, enabling a complete gene expression profiling of Las genome and revealing unique transcriptomic patterns compared with previous studies using midrib tissues. Comparative transcriptomics between seed coat, midrib, and ACP identified specific responses and metabolic states of Las in different host tissue. Two Las virulence factors that exhibit higher expression in seed coat can suppress callose deposition. Therefore, they may contribute to unclogging sieve plate pores during Las colonization in seed coat vasculature. Furthermore, analysis of regulatory elements uncovers a potential role of LuxR-type transcription factors in regulating Liberibacter effector gene expression during plant colonization. Together, this work provides novel insights into the pathogenesis of the devastating citrus HLB. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Active Compared With Passive Voiding Trials After Midurethral Sling Surgery: A Systematic Review
Authors: Drangsholt Siri , Lleras Cassidy , Kindler Rebecca , Babajanian Maytal , Pollack Bracha , Harutyunyan Aleksandr , Grunfeld Matan , Gejerman Yehuda , Kilkenny Katherine , Bulmash Moshe , Hirani Rahim , Alerasool Parissa , McNeil Christopher , Popiel Patrick , Drugge Elizabeth D. , Grimes Cara 
Published: November 2024

(Abstracted from Obstet Gynecol 2024;143(5):633–643)
Up to 14% of younger women and 35% of older women are affected by stress urinary incontinence, which is commonly addressed using mesh midurethral sling surgery, with a cure rate of up to 84%. One potential adverse effect of this surgery is postoperative urinary retention, which is defined as an inability to voluntarily pass enough urine after a surgical procedure to avoid catheterization.
Salvianolate injection ameliorates cardiomyopathy by regulating autophagic flux through miR-30a/becn1 axis in zebrafish
Authors: Li Jianxuan , Zhang Yang , Zuo Zhi , Zhang Zhenzhong , Wang Ying , Chang Shufu , Huang Jia , Dai Yuxiang , Ge Junbo 
Published: November 2024

Abstract

Background:
Salvianolate is a compound mainly composed of salvia magnesium acetate, which is extracted from the Chinese herb Salvia miltiorrhiza. In recent years, salvianolate injection has been widely used in the treatment of cardiovascular diseases, but the mechanism of how it can alleviate cardiotoxicity remains unclear.

Methods:
The cardiac injury model was constructed by treatment with doxorubicin (Dox) or azithromycin (Azi) in zebrafish larvae. Heart phenotype, heart rate, and cardiomyocyte apoptosis were observed in the study. RNA-seq analysis was used to explore the underlying mechanism of salvianolate treatment. Moreover, cardiomyocyte autophagy was assessed by in situ imaging. In addition, the miR-30a/becn1 axis regulation by salvianolate was further investigated.

Results:
Salvianolate treatment reduced the proportion of pericardial edema, recovered heart rate, and inhibited cardiomyocyte apoptosis in Dox/Azi-administered zebrafish larvae. Mechanistically, salvianolate regulated the lysosomal pathway and promoted autophagic flux in zebrafish cardiomyocytes. The expression level of becn1 was increased in Dox-induced myocardial tissue injury after salvianolate administration; overexpression of becn1 in cardiomyocytes alleviated the Dox/Azi-induced cardiac injury and promoted autophagic flux in cardiomyocytes, while becn1 knockdown blocked the effects of salvianolate. In addition, miR-30a, negatively regulated by salvianolate, partially inhibited the cardiac amelioration of salvianolate by targeting becn1 directly.

Conclusion:
This study has proved that salvianolate reduces cardiomyopathy by regulating autophagic flux through the miR-30a/becn1 axis in zebrafish and is a potential drug for adjunctive Dox/Azi therapy.
A Novel Artificial Endothelial Replacement Membrane for the Treatment of Chronic Corneal Edema
Authors: Daphna Ofer , Auffarth Gerd U. , Lapid-Gortzak Ruth , Chaurasia Sunita , Gilboa Efrat , Lemze Anat , Dover Michael , Marcovich Arie L. 
Published: November 2024

Purpose:
The purpose of this study was to report the safety and efficacy results of an artificial lamellar implant for the treatment of chronic corneal edema.

Methods:
The EndoArt (EyeYon Medical, Ness Ziona, Israel), an artificial endothelial replacement membrane designed to treat corneal edema, was implanted in 24 eyes of 24 patients with low-to-normal visual potential. We present the safety and efficacy results from a prospective, open-label, single-arm, multicenter study conducted over a 12-month period.

Results:
Twenty-four patients were enrolled, with no device-related serious adverse events reported. Seventeen patients completed 12-month follow-up, showing a reduction in average central corneal thickness from 759 ± 116 μm to 613 ± 135 μm. Best-corrected distance visual acuity improved from 1.88 ± 0.79 logarithmic minimum angle of resolution (logMAR) to 1.34 ± 0.57 logMAR. Sixty percent gained at least 3 early treatment diabetic retinopathy study (ETDRS) lines. The EndoArt was removed in 5 cases due to incomplete attachment and replaced by corneal transplants; 1 patient was lost to follow-up, and 1 had a procedure failure. No device-related long-term complications, infections, or inflammations were reported. The implants remained transparent throughout the study.

Conclusions:
The first-in-human results of EndoArt implantation demonstrated the device's potential to treat patients suffering from corneal edema with a favorable safety profile and effective edema reduction in most subjects, with no device-related serious adverse event. The EndoArt may offer a viable solution in regions facing a shortage of donor corneas, as well as for patients who have poor prognosis with human tissue.
Vision-Related Quality of Life After Fyodorov–Zuev Keratoprosthesis in China
Authors: Li Yan , Mei Lina , Zhuo Deyi , Wu Jie , Xu Weiwei , Wang Qun , Wang Yu-qi , Li Manmei , Wang Xiu , Feng Kexuan , Du Cuimei , Feng Huiping , Huang Yifei , Wang Liqiang 
Published: November 2024

Purpose:
The purpose of this study was to assess vision-related quality of life (VR-QoL) and its determinants in patients with implanted Fyodorov–Zuev (MICOF) KPro.

Methods:
Eighty-eight patients with bilateral corneal blindness who underwent unilateral Fyodorov–Zuev (MICOF) KPro implantation were included in this cross-sectional study. VR-QoL was assessed using the National Eye Institute Visual Function Questionnaire-25 (NEI-VFQ-25). Univariate analyses and multivariate linear regression were used to identify VR-QoL determinants.

Results:
Eighty-eight patients, aged 50.00 ± 13.72 years, with a mean postoperative time of 5.13 ± 3.31 years, were included. The composite NEI-VFQ-25 score improved from 17.04 ± 5.60 to 46.00 ± 19.21 (P < 0.01) after Fyodorov–Zuev (MICOF) KPro implantation. The multivariate linear regression analysis results showed that the postoperative composite score was correlated with postoperative visual acuity (VA) of the operative eye (B = −21.66, P < 0.01). Improvement in NEI-VFQ scores was associated with better postoperative VA of the operative eye (B = −18.67, P < 0.01) and poorer preoperative VA of the fellow eye (B = 7.11, P < 0.05).

Conclusion:
VR-QoL in patients with bilateral corneal blindness was significantly impaired but improved after Fyodorov–Zuev (MICOF) KPro implantation. Improvement in VR-QoL was inversely associated with postoperative VA of the operative eye but positively correlated with preoperative VA of the fellow eye. This study affirmed that Fyodorov–Zuev (MICOF) KPro was an effective alternative for patients with corneal blindness with respect to VR-QoL.
Crescentic Autograft—Graft Salvage Technique for Undersized Donor Lenticules in Superficial Anterior Lamellar Keratoplasty
Authors: Bari Aafreen , Nair Sridevi , Sharma Namrata , Sinha Rajesh , Agarwal Tushar 
Published: November 2024

Purpose:
To describe the use of crescentic autograft as a graft salvage technique for inadvertently undersized donor lenticules in superficial anterior lamellar keratoplasty (SALK).

Methods:
A prospective interventional study included 5 eyes of 4 patients who underwent SALK and had an inadvertently undersized donor lenticule. The described technique involves the preparation of a crescent-shaped graft from the host lenticule itself that is matched to fit into the remaining bare recipient stromal bed.

Results:
At 6-week follow-up, the crescentic autograft was uniformly epithelized along with the donor tissue. There were no incidences of graft dehiscence, rejection, or infection.

Conclusions:
Crescentic autograft from the host lenticule offers a simple, easy-to perform, and unique technique to manage cases of undersized donor lenticules in SALK with no additional risks.
Health Center Characteristics Associated With Hospital Care Among Medicare-Medicaid Dual Enrollees
Authors: Wright Brad , Lombardi Brianna , Akiyama Jill , Potter Andrew J. , Sabik Lindsay M. , Stehlin Grace G. , Trivedi Amal N. , Wolinsky Fredric D. 
Published: November 2024

Using 2012–2018 Medicare claims and health center data, we identified factors associated with variation in rates of hospital care among Medicare–Medicaid dual enrollees receiving primary care at health centers. In our sample (n = 5961 health center-years), we found no evidence that patient-centered medical home designation or other modifiable health center characteristics were associated with reductions in hospital care use, which depends more on health center patient mix. Thus, policymakers should target efforts to health centers serving the most disadvantaged and marginalized communities.
The Development of Doctor-nurse-patient Co-construction through Evidence-based Narrative Exploration of Traditional Chinese Medicine
Authors: YU Miao , LI Bo , LIU Qian , WANG Tianyuan 
Published: November 2024

Abstract
Traditional Chinese medicine (TCM) is the wisdom crystallization and cultural treasure of the Chinese nation. Establishing a positive relationship among doctors, nurses, and patients is essential for promoting the sustainable development of TCM culture. As an important branch of evidence-based narrative medicine, evidence-based narrative of TCM significantly contributes to the co-construction of doctors, nurses and patients. This paper discusses the necessity and clinical practice value of promoting co-construction of doctors, nurses and patients based on the principles of evidence-based narrative TCM. By working together to select optimal diagnosis and treatment options, we aim to enhance patient compliance, alleviate tensions among the parties involved, and establish equitable collaborative relationships. Creating a harmonious medical environment is crucial for promoting the continuous development of this collaborative model in clinical practice, thereby improving patients’ overall health, increasing public recognition of TCM, and supporting its sustainable growth.
The Narrative Medicine Practice for the Treatment of Ben Tun Qi
Authors: WANG Zixu , LIU Yipin 
Published: November 2024

Abstract
This article takes the narrative medicine practice of traditional Chinese medicine (TCM) in the treatment of Ben Tun Qi (奔豚气) as an example to illustrate the characteristics of narrative medicine in TCM diagnosis and treatment, and strives to form a simple, easy to implement, and practical process of narrative medicine of TCM, which can help doctors provide more comprehensive, detailed, and humanistic care for diagnosis and treatment activities, and achieve doctor-patient harmony.
A Narrative Review of Congenital Syphilis in the United States: Innovative Perspectives on a Complex Public Health and Medical Disease
Authors: Lehman Alice , Olson Nehemiah , Foster Jill , Contag Stephen 
Published: November 2024

Abstract
Over the past two decades, congenital syphilis cases have risen 11-fold in the United States. While disparities across geography, race, and ethnicity exist, lack of timely screening or treatment is identified in 88% of cases nationally. Congenital syphilis is a public health and medical problem rooted in systematic and societal structural determinants of health and healthcare limitations. Early syphilis in pregnancy leads to congenital syphilis if untreated in 50 – 70% of cases, with risk for fetal demise, and among survivors, congenital anomalies, organ damage, and central nervous system disease. Prevention of congenital syphilis lies in early detection and treatment in pregnant persons. In this narrative review, we describe the evolving epidemiology of syphilis and congenital syphilis, highlighting unique aspects among women. We explore the role of novel screening and treatment strategies, public health policy, and medical considerations in terms of congenital syphilis prevention. Readers of this review will understand CS as a complex public health and medical disease that can be prevented through innovative and coordinated strategies in public health policy, expanded screening and research opportunities.
Exploring cognitive overload in adults with visual impairment: The association between concentration and fatigue
Authors: Veldman Manon H. J. , Adanç Beritan , van Rens Ger H. M. B. , van Nispen Ruth M. A. , van der Aa Hilde P. A. 
Published: November 2024

SIGNIFICANCE
This research contributes to the concept of cognitive overload and the association between concentration and fatigue in individuals with visual impairment (VI). Gaining more insight into these and other factors explaining cognitive overload is essential for a better understanding, prevention, and treatment of fatigue severity in individuals with VI.

PURPOSE
Individuals with VI need to use additional cognitive resources to compensate for their loss of vision. These cognitive demands include increased concentration, which may put an extra burden on energy levels. This study aimed to expand upon previous research on the concept of cognitive overload by exploring the relationship between concentration and fatigue severity in individuals with VI.

METHODS
A cross-sectional study was conducted based on data from the eligibility screening of the E-nergEYEze trial (n = 213). Concentration problems were measured with the Checklist of Individual Strengths subscale Concentration, and fatigue severity was measured with the Checklist of Individual Strengths subscale Fatigue Severity. Linear regression analyses were performed to investigate the association between concentration problems and fatigue severity. Potential effect modifiers (i.e., sociodemographic and vision-related characteristics) were considered, and associations were corrected for confounding.

RESULTS
Concentration problems were directly and positively associated with fatigue severity (β = 0.47; R
2 = 0.10; 95% confidence interval, 0.28 to 0.66; adjusted model: β = 0.52; R
2 = 0.16; 95% confidence interval, 0.32 to 0.72). No relevant effect modifiers were identified. However, other factors that were not included in this study were involved in this association.

CONCLUSIONS
This study contributes to understanding the cognitive overload as experienced by adults with VI and highlights the importance of the association of concentration problems, as a reflection of the cognitive burden and perceived fatigue. It is recommended to identify and address concentration difficulties through suitable awareness and treatment strategies to prevent and reduce severe fatigue in this population.
The Complexity of Health Self-Management Behavior. Beliefs and Attitudes of Individuals Living With Spinal Cord Injury in Switzerland
Authors: Diviani Nicola , Qama Enxhi , Brach Mirjam , Gemperli Armin , Jordan Xavier , Scheel-Sailer Anke , Rubinelli Sara 
Published: November 2024

Objective
Within the objective of defining targets for interventions to support self-management behaviors among people living with spinal cord injury, this study aims to describe self-management attitudes and beliefs in community-dwelling people living with spinal cord injury in Switzerland and to identify their correlates in terms of personal characteristics and outcomes, such as secondary health conditions and quality of life.

Design
This is a cross-sectional, observational study using data from the third community survey of the Swiss Spinal Cord Injury Cohort Study.

Results
Out of 1158 individuals, the data revealed high endorsement in areas of prevention importance, perceived knowledge, adherence to recommendations, and proactive problem-solving. Personal and lesion characteristics were significantly associated with differing self-management beliefs and attitudes. Overall, a more positive and proactive stance toward self-management correlated with enhanced outcomes. However, readiness to renounce activities to adhere to self-management recommendations was associated with lower quality of life.

Conclusions
The findings confirm the complex nature of self-management among individuals with spinal cord injury, advocating for a tailored, person-centered approach in designing interventions. Highlighting a potential trade-off between strict self-management adherence and quality of life outcomes posits an avenue for balanced, individualized self-management strategies to ameliorate health outcomes and life quality for individuals with spinal cord injury.
Healthcare Service Utilization and Related Costs. A Comparison of the Situation of Persons With Spinal Cord Injury to the General Population
Authors: Pacheco Barzallo Diana , Oña Ana , Brach Mirjam , Gemperli Armin 
Published: November 2024

Objective
The aim of the study is to investigate healthcare utilization and related costs in spinal cord injury compared to the Swiss population, addressing gaps in understanding this vulnerable group’s needs.

Design
We analyzed healthcare utilization across age groups using data from the Swiss Health Survey and the Swiss Community Survey. Employing count models, Zero-Inflated Negative Binomial, and Poisson regressions, we estimated the adjusted healthcare visits across different age groups in the sample.

Results
Persons with spinal cord injury use 11 times more healthcare services than the healthy population and 4 times more than persons with other chronic health conditions. Notably, service providers for persons with spinal cord injury include physiotherapists, nursing services, general practitioners, and specialists. The high frequency of usage of persons with spinal cord injury translates into high costs, which is 3 times higher than in the general healthy population. These patterns become more evident as persons with spinal cord injury age, where nursing care and rehabilitation services become the most demanded services.

Conclusions
Our findings show the multiple healthcare needs of people with spinal cord injury, which makes them a high-needs, high-cost group. This characteristic underscores the importance of preventive measures to reduce the incidence of spinal cord injury in older adults.