bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inference of past demography, dormancy and self-fertilization rates from whole genome sequence data
Authors: Sellinger Thibaut , Abu Awad Diala , Möst Markus , Tellier Aurélien 
Published: July 2019

AbstractSeveral methods based on the Sequential Markovian Coalescent (SMC) have been developed to use full genome sequence data to uncover population demographic history, which is of interest in its own right and a key requirement to generate a null model for selection tests. While these methods can be applied to all possible species, the underlying assumptions are sexual reproduction at each generation and no overlap of generations. However, in many plant, invertebrate, fungi and other species, those assumptions are often violated due to different ecological and life history traits, such as self-fertilization or long term dormant structures (seed or egg-banking). We develop a novel SMC-based method to infer 1) the rates of seed/egg-bank and of self-fertilization, and 2) the populations’ past demographic history. Using simulated data sets, we demonstrate the accuracy of our method for a wide range of demographic scenarios and for sequence lengths from one to 30 Mb using four sampled genomes. Finally, we apply our method to a Swedish and a German population ofArabidopsis thalianademonstrating a selfing rate ofca.0.8 and the absence of any detectable seed-bank. In contrast, we show that the water fleaDaphnia pulexexhibits a long lived egg-bank of three to 18 generations. In conclusion, we here present a novel method to infer accurate demographies and life-history traits for species with selfing and/or seed/egg-banks. Finally, we provide recommendations on the use of SMC-based methods for non-model organisms, highlighting the importance of the per site and the effective ratios of recombination over mutation.
Prevalence of Chlamydia trachomatis and Candida albicans; hospital based study
Authors: Njeru Albert , Mwafaida Joseph 
Published: July 2019

AbstractBackgroundChlamydia and candidiasis have only mild or no symptoms at all. When symptoms develop, they are often mistaken for something else like urinary tract infections or yeast infections. These infections affect both men and woman of all backgrounds and economic levels.ObjectiveThe prevalence of Chlamydia trachomatis (C.trachomatis) and Candida albicans (C.albicans) infections among attendees of Kilifi Medical Centre in Kilifi county, Kenya was studied.MethodologyUrethral and vaginal swabs samples were aseptically collected from 305 subjects, examined for Candida albicans and Chlamydia trachomatis using standard microbiological methods. The swabs were analyzed using direct wet smears, Gram-stained smear and culture techniques.ResultsOf the 305 participants, 181 (59.34%) females and 124 (40.66%) males were tested with the overall prevalence of 53.44 % for both Chlamydia trachomatis and Candida albicans with females having a higher infection rate (35.14 %) for chlamydia and candidasis than men (17.71). Amongst the different age groups investigated, candida and chlamydia distribution was highest in participants aged 28-32 years (21.97 %).The infection rate of C.trachomatis (14.43 %) among the male participants was higher than the infection rate revealed among the female participants of 1.97 % while the infection rate of C.albicans was higher among the female participants (33.77 %) compared to the 3.28 % recorded in male participants with no co-infections revealed.ConclusionThe results of this study demonstrated a significant difference between male and female chlamydia and candida infections with women being severely affected than men. The study recommended routine screening for sexually transmitted infections (STIs) which is essential in preventing infections transmissions, assessment of the role of socio-demographic and behavioral risks on Chlamydia trachomatis and Candida albicans, proper treatment of all candida and chlamydia by use of correct/effective medicines, contact tracing and treatment of sexual partners and health education.
Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems
Authors: Arora Deepanksha , Abel Nikolaj B. , Liu Chen , Van Damme Petra , Yperman Klaas , Vu Lam Dai , Wang Jie , Tornkvist Anna , Impens Francis , Korbei Barbara , Eeckhout Dominique , Van Leene Jelle , Goossens Alain , De Jaeger Geert , Ott Thomas , Moschou Panagiotis , Van Damme Daniël 
Published: July 2019

AbstractProximity-dependent biotin labelling (PDL) uses a promiscuous biotin ligase (PBL) or a peroxidase fused to a protein of interest. This enables covalent biotin labelling of proteins and allows subsequent capture and identification of interacting and neighbouring proteins without the need for the protein complex to remain intact. To date, only few papers report on the use of PDL in plants. Here we present the results of a systematic study applying a variety of PDL approaches in several plant systems using various conditions and bait proteins. We show that TurboID is the most promiscuous variant in several plant model systems and establish protocols which combine Mass Spectrometry-based analysis with harsh extraction and washing conditions. We demonstrate the applicability of TurboID in capturing membrane-associated protein interactomes usingLotus japonicussymbiotically active receptor kinases as test-case. We further benchmark the efficiency of various PBLs in comparison with one-step affinity purification approaches. We identified both known as well as novel interactors of the endocytic TPLATE complex. We furthermore present a straightforward strategy to identify both non-biotinylated as well as biotinylated peptides in a single experimental setup. Finally, we provide initial evidence that our approach has the potential to infer structural information of protein complexes.
A role for the P2Y1 receptor in nonsynaptic cross-depolarization in the rat dorsal root ganglia
Authors: Carvalho Gil B. , Mulpuri Yatendra , Damasio Antonio , Spigelman Igor 
Published: July 2019

AbstractNon-synaptic transmission is pervasive throughout the nervous system. It appears especially prevalent in peripheral ganglia, where non-synaptic interactions between neighboring cell bodies have been described in both physiological and pathological conditions, a phenomenon referred to as cross-depolarization (CD) and thought to play a role in sensory processing and chronic pain. CD has been proposed to be mediated by a chemical agent, but its identity has remained elusive. Here, we report that in the rat dorsal root ganglion (DRG), the P2Y1 purinergic receptor (P2RY1) plays an important role in regulating CD. The effect of P2RY1 is cell-type specific: pharmacological blockade of P2RY1 inhibited CD in A-type neurons while enhancing it in unmyelinated C-type cells. In the nodose ganglion of the vagus, CD requires extracellular calcium in a large percentage of cells. In contrast, we show that in the DRG extracellular calcium appears to play no major role, pointing to a mechanistic difference between the two peripheral ganglia. Furthermore, we show that DRG glial cells also play a cell-type specific role in CD regulation. Fluorocitrate-induced glial inactivation had no effect on A-cells but enhanced CD in C-cells. These findings shed light on the mechanism of CD in the DRG and pave the way for further analysis of non-synaptic neuronal communication in sensory ganglia.HighlightsThe purinergic receptor P2RY1 plays a regulatory role in non-synaptic crossdepolarization (CD) in the mammalian DRGThe effect of P2RY1 is cell type-specific: it enhances CD in myelinated A-type neurons, but inhibits it in unmyelinated C-cellsCD in the DRG does not require extracellular calcium. This is in contrast with the nodose ganglion, where extracellular calcium plays an important role in nonsynaptic interactionsCD is also modulated by DRG glial cells. Glia selectively inhibit CD in C-type neurons
Comparison of Non-linear and Linear Models of Single Channel EEG in patients and normal subjects
Authors: ZhuoJun Gu , ZhiQiang Huang , Xiao Zhu , ShenXun Shi 
Published: July 2019

AbstractThis article examines the possibility of using non-linear models(Support Vector Regression) to model the single channel EEG signals from psychiatric patients and a group of normal participants, to predict psychology trait ratings, like attention, anxiety, alertness, fatigue, sleepiness and depression. It used linear models as benchmarks, and the results showed non-linear models outperformed the benchmarks, as well as more advanced linear methods, like principle component regression. It is thus concluded that using single channel in practical situations to monitor these traits would be possible.
VariantSpark, A Random Forest Machine Learning Implementation for Ultra High Dimensional Data
Authors: Bayat Arash , Szul Piotr , O’Brien Aidan R. , Dunne Robert , Luo Oscar J. , Jain Yatish , Hosking Brendan , Bauer Denis C. 
Published: July 2019

AbstractThe demands on machine learning methods to cater for ultra high dimensional datasets, datasets with millions of features, have been increasing in domains like life sciences and the Internet of Things (IoT). While Random Forests are suitable for “wide” datasets, current implementations such as Google’s PLANET lack the ability to scale to such dimensions. Recent improvements by Yggdrasil begin to address these limitations but do not extend to Random Forest. This paper introduces CursedForest, a novel Random Forest implementation on top of Apache Spark and part of the VariantSpark platform, which parallelises processing of all nodes over the entire forest. CursedForest is 9 and up to 89 times faster than Google’s PLANET and Yggdrasil, respectively, and is the first method capable of scaling to millions of features.
A novel hybrid control approach for modular automation system: a case study of sensorless interior permanent magnet in-wheel motor
Authors: Li Yong , Huang Yanjun , Xu Xing 
Published: July 2019

Purpose
Sensorless interior permanent magnet in-wheel motor (IPMIWM), as an exemplar of modular automation system, has attracted considerable interests in recent years. This paper aims to investigate a novel hybrid control approach for the sensorless IPMIWM from a cyber-physical systems (CPS) perspective.

Design/methodology/approach
The control approach is presented based on the hybrid dynamical theory. In the standstill-low (S-L) speed, the rotor position/speed signal is estimated by the method of the high frequency (HF) voltage signal injection. The least square support vector machine (LS-SVM) is used to acquire the rotor position/speed signal in medium-high (M-H) speed operation. Hybrid automata model of the IPMIWM is established due to its hybrid dynamic characteristics in wide speed range. A hybrid state observer (HSO), including a discrete state observer (DSO) and a continuous state observer (CSO), is designed for rotor position/speed estimation of the IPMIWM.

Findings
The hardware-in-the-loop testing based on dSPACE is carried out on the test bench. Experimental investigations demonstrate the hybrid control approach can not only identify the rotor position/speed signal with a certain load but also be able to reject the load disturbance. The reliability and the effectiveness of the proposed hybrid control approach were verified.

Originality/value
The proposed hybrid control approach for the sensorless IPMIWM promotes the deep combination and coordination of sensorless IPMIWM drive system. It also theoretically supports and extends the development of the hybrid control of the highly integrated modular automation system.
Why the new orange juice consumption model favors global trade and growth in orange production
Authors: Allegra Valeria , Zarbà Carla , La Via Giovanni , Zarbà Alfonso Silvio 
Published: July 2019

Purpose
The purpose of this paper is to analyze international orange trade as different types of orange juice seem to obtain the favor of newer food consumption patterns as opposed to fresh oranges. In addition, the authors will outline market tendencies that could be useful to stakeholders interested in any way in the sector.

Design/methodology/approach
This document uses the Lafay index (LFI) in order to evaluate the intra-sectoral trade specialization; however, the social network analysis (SNA) approach is used to connect the international trade relations and to refine, classify and prioritize the countries having a central role in the orange juice world trade network. For both indexes, UNCOMTRADE has been used.

Findings
The findings show that the growing orange juice trade is the leading global growth factor as opposed to the sale of fresh oranges. It appears that major trade revenues come from orange juice concentrate (Frozen Concentrated Orange Juice (FCOJ)). Business takes place mostly in Europe.

Originality/value
This study shows for the first time that the current orange juice export competitiveness is crucial to the long-term survival of the orange sector. This study valuably contributes to the less known literature regarding FCOJ and Not From Concentrate orange juice trade relevance.
Framework contracts for medicine inventory management in public healthcare
Authors: Jalil Muhammad Naiman , Malik Wafa , Javaid Areeb , Khan Ali Jan 
Published: July 2019

Learning outcomes
This paper aims to highlight the implications of financial planning for public procurement process for medicine purchase. The purpose of this case is also to understand how the choice of contract type in public procurement impacts medicine inventory levels and availability. It finally highlights the appropriate configuration of framework procurement contract for procurement of discrete goods in the context of public sector procurement.

Case overview/synopsis
Primary and Secondary Healthcare Department (P&SHD), Government of the Punjab provides free public health-care services in the Punjab province. Public health-care services of P&SHD are organised in a tiered manner with almost 3,000 primary and secondary medical facilities dispersed throughout the Punjab province. P&SHD maintains inventories of approximately 300 medicines to support medical service provision.

Complexity academic level
This case can be taught in procurement and inventory management module of MBA level operations management course. It can also be used in executive course on public sector procurement management. The case aims to highlight the interrelation between inventory planning and procurement management process. Hence, it should be used after participants have understood inventory models, procurement process and procurement contract types. Standard readings or cases on inventory and procurement management that cover topics such economic order quantity, procurement process steps and procurement contracts can be used to develop this understanding.

Supplementary materials
Teaching Notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes.

Subject code
CSS 9: Operations and logistics.
Exploring entrepreneurial orientation and intentions among technical university students
Authors: Sahoo Swagatika , Panda Rajeev Kumar 
Published: July 2019

Purpose
The purpose of this paper is to empirically investigate the impact of the contextual antecedents on the individual entrepreneurial orientation (IEO) of university graduates, which, in turn, affects their entrepreneurial intentions (EIs).

Design/methodology/approach
Primary data were collected in the form of 510 valid responses from engineering students across two technical universities in India, through a structured questionnaire consisting of scales adapted from the extant literature, and the data were empirically validated in this study. The reliability and validity measures of the constructs were validated through the confirmatory factor analysis, and the proposed hypotheses were validated using structural equation modelling.

Findings
The results of this empirical analysis validate that the contextual antecedents have a significant positive impact on students’ entrepreneurial orientation (EO), which, in turn, has a significant positive influence on EIs.

Research limitations/implications
This analysis depicts the significance of EO as a perceptual driver at the individual level and substantiates that the availability of resources such as startup capital, access to business information, social networks and supportive university context significantly affects the decision-making process of an individual to venture into an otherwise uncertain occupation of entrepreneurship.

Practical implications
The study has the likely potential to help university administrators and policymakers to allocate resources, develop strategies and provide effective entrepreneurial learning in entrepreneurship-oriented courses aimed at honing entrepreneurial skills and self-confidence of the university students. This holistic model can be used as a tool for resource planning and prioritising in order to provide the desired contextual support essential for fostering the IEO of the university students towards adopting entrepreneurial career, thereby assisting them to achieve their career goals and the broader objective of nation-building.

Originality/value
This study adopts an innovative approach to empirically validate the EO construct at the individual level, which has been studied at the organisation (firm) level till today. This research explores the relevant contextual antecedents and analyses their impact on IEO as well as the explanatory capacity of IEO to explain students’ EIs in the contextual backdrop of universities in a fast transitioning economy like India.
Characterising the airtightness of dwellings
Authors: Ashdown Minnie M.A. , Crawley Jenny , Biddulph Phillip , Wingfield Jez , Lowe Robert , Elwell Clifford A. 
Published: July 2019

Purpose
The purpose of this paper is to investigate the distribution of dwelling airtightness test results for a developer, between 2007 and 2011. The changes in airtightness test results over time are discussed, and links between the airtightness test results and the construction technique are investigated.

Design/methodology/approach
A data set of airtightness test results was statistically analysed by applying probabilistic model of the distribution and using Bayesian parameter estimation techniques.

Findings
The inferred background distributions, those estimated to describe dwelling performance before secondary sealing, suggest an improvement in airtightness between 2008 and 2011, the mode decreases from 5.46±0.09 m3/m2h to 4.12±0.07 m3/m2h with a corresponding shift in practice towards a more target-driven approach. The most airtight dwellings are constructed from reinforced concrete frame, followed by “traditional” (dry lined masonry), timber frame and lightweight steel frame.

Research limitations/implications
This study is limited by the size of the available data set (901 dwellings), and by the fact that the data set contains a larger proportion of flats to houses; however, the metadata has enabled the exploration of the link between construction practices and airtightness.

Practical implications
Developers need better guidance surrounding how to meet more stringent airtightness requirements through improvements to the primary air barrier, with incentives and support to deliver changes in practice. Furthermore, if a large number of dwellings undergo secondary sealing, this may have implications for the long-term efficiency of the dwelling stock.

Originality/value
This analysis investigates two issues that have not previously been studied on a significant number of dwellings: the changes to the distribution of airtightness results over time and the link between construction methods and airtightness.
“Think sustainable, act local” – a stakeholder-filter-model for translating SDGs into sustainability initiatives with local impact
Authors: Raub Steffen P. , Martin-Rios Carlos 
Published: July 2019

Purpose
The purpose of this paper is to develop and illustrate a comprehensive framework for how hospitality firms can overcome the broad vs narrow dilemma in sustainable management. The authors develop a framework for how to break down the United Nations sustainable development goals (SDGs) into actionable and context-specific subsets and select individual sustainability initiatives with maximum impact.

Design/methodology/approach
The framework focuses on sustainable innovation and management in hospitality and the issue-focused stakeholder perspective. The authors develop a theoretical framework for the selection of impactful sustainability initiatives in the hospitality industry. In addition, the paper provides a broad range of concrete examples for how different stakeholders can act as barriers or catalysts for the implementation of sustainability initiatives.

Findings
The major contribution of this paper is twofold. First, it recognizes that the solutions to the great sustainability challenges ahead involve the active participation of the hospitality industry in establishing partnerships with stakeholders. Second, it offers an ambitious roadmap for hospitality firms to identify local issues specific to sustainable management actions committed to advancing the social, environmental and economic dimensions of sustainability.

Originality/value
The framework has considerable practical implications in that it is centered on helping hospitality firms select an appropriate set of SDGs for their local context and translate them into specific sustainability initiatives that address these goals. The “stakeholder-filter model” methodology is aligned with an approach that is already being used for the development of sustainability initiatives outside the scope of the hospitality industry. As a result, the framework should have substantial practical value for the hospitality industry.
The impact of age on gay consumers’ reaction to the physical and social servicescape in gay bars
Authors: Olson Eric , Park Heelye (Jason) 
Published: July 2019

PurposeThe purpose of this study is to examine the effects of physical servicescape, social servicescape and age on gay consumers’ evaluations of a LGBT advertisement of a gay bar of a gay bar.Design/methodology/approachA 2 × 2 × 2 experimental design was used to test the effects with a sample of gay males in the USA. Data were analyzed using ANCOVA and bootstrapping mediation.FindingsResults of this study indicate a statistically significant three-way interaction effect of the two independent variables and age on the gay bar’s perceived LGBT (lesbian/gay/bisexual/transgender)-friendliness. Perceived friendliness mediated the effects of the independent variables on behavioral intentions. Furthermore, the mediation effect was moderated by the age cohort.Research limitations/implicationsThe findings indicate a changing perception of gay servicescape between the older and younger gay men. Implications for hospitality managers are provided.Originality/valueThis research contributes to the servicescape literature by expanding the realm of research to gay servicescape and gay consumers, an emergent and more visible hospitality segment.