bluuebunny/crossref_metadata_2025_split huggingface dataset:
Altered placental expression of genes related to Vitamin D Metabolism and their correlation with spiral artery remodeling in Preeclamptic Women
Authors: Goswami Pushpa , Memon Samreen , Ujjan Ikram Din , Gul Fahmida 
Published: November 2024

Background: Several studies reveals the correlation of preeclampsia (PE) with low vitamin D (VD) level due to disturbed trophoblast invasion and failure of spiral artery remodeling. The metabolism of VD is regularized by it signaling molecules expressed by the placenta during pregnancy. Therefore, this study was designed to assess and compare the levels of VD and it signaling molecules in placentae from normotensive and preeclamptic pregnancies and their correlation with spiral artery remodeling.Methods: This study was carried out at Department of Anatomy at Liaquat University of Medical & Health Sciences, Jamshoro after ethical approval on placentae of 122 parturients; 61 from normal and 61 from preeclampsia, data was collected on predesigned proforma and was analyzed on SPSS 23.Results: The mean and standard deviation of maternal age, gestational age, and parity was 32.73±5.9 years, 37.3±3.6 weeks and 3±1 respectively. The wall thickness of SA and VD when compared in two groups found statically significant. Vitamin D signaling molecules were determined by immunostaining compared in two groups by applying Mann Whitney U test and Pearson’s correlation which reveals significant differences in preeclamptic versus controls.Conclusions: Despite extensive research indicating a link between low levels of VD and its signaling molecules in the context of inadequate spiral artery remodeling, there is still a need for further investigation to fully understand the mechanisms underlying this association in preeclampsia.Keywords: Preeclampsia; Remodeling; Spiral artery; Vitamin D; Vitamin D signaling molecules
Impact of varying sorbitol concentrations on hematological and biochemical blood parameters in Wistar rats (Rattus norvegicus)
Authors: Al-Moussawi Zahraa H.K. , Almahdawi Mozhir K.K. , Al-Bayati Abdul Sattar S.S. 
Published: November 2024

Background: Sorbitol is an artificial sweetener that is very similar to natural sugar, but it is sweeter than natural sugar and lower in calories. Sorbitol is used as an alternative to natural sugar, and it is extracted from regular glucose. It does not have any negative effects on the animals’ health, and it is characterized in safe use in many scientific experiments for the diet of humans and animals.Methods: This study was conducted in the animal house, College of Veterinary Medicine, Al-Qasim Green University for a period of 90 days. Forty rats of the Wistar strain (Rattus norvegicus) were randomly distributed into 4 groups, namely the first group (control group) while the second, third and fourth groups were treated with 100, 200 and 300 μg of sorbitol/kg of body weight .The rats were iso in live weight and age at the beginning of the experiment. Blood samples were drawn at the end of the experimental period. Results: Increase in level dose of sorbitol resulted significant improvement in accounts of red blood cells, white blood cells, and a significant increase in level of hemoglobin, lymphocytes, and monocytes for second, third, and fourth groups compared to the control group. Moreover, the significant improvement in most of the blood parameters was reflected to significant increase in clotting and bleeding time in the second, third and fourth groups of rats compared to the control group. Also, the results showed a significant increase in the levels of FSH, LH, testosterone, Inhibin and MAD hormones for the second, third and fourth groups compared to the control group.Conclusions: The results of the current study revealed that sorbitol has many benefits in the diet of humans and animals and has an effect on blood indicators, some sex hormones and antioxidants in the body. It is recommended not to consume excessive sorbitol in the diet.Keywords: Sorbitol; Hematological parameters; FSH; LH; Wistar rats
Beneficial impacts of natural antioxidants on molecular structure and parameters of human sperm
Authors: Kadhum Rafed Abbas , Sfaieh Aqads Mohammed , Salih Asmaa Musaed 
Published: November 2024

Background: Sexual dysfunctions are often associated with oxidative stress, DNA damage, and reduced levels of sex hormones.Methods: In this study, a combination of polyfloral honey, royal jelly (RJ), Korean ginseng, and palm pollen were tested to reduce oxidative DNA damage in semen. The study involved 25 male patients aged 22-45 years who were participating in a fertility clinic in Wasit, South-East Iraq. Surgically resolvable problems were excluded, as well as subjects who smoked.Results: Semen analysis and serum total oxidant status were used to assess the fertility potential in males, and pregnancy occurrence served as evidence of sperm ability for fertilization. Our findings indicate that consuming honey with Korean ginseng and palm pollen improved sperm count by reducing caspase-3 activity and protecting against oxidative DNA damage. In addition, forward progressive motility and morphology of the sperm were significantly improved by the treatment and also had a direct effect on mitochondrial enzymes.Conclusion: Therefore, the use of honey combined with specific herbals appears to be a promising treatment option for the improvement of sperm parameters to treat male infertility.Keywords: Antioxidants; Oxidative DNA damage; Honey; Royal jelly; Korean ginseng; Post-acrosomal lamina
Identification and control of toxin-producing fungus in wheat grain stores using environment friendly factors
Authors: Jubair Ali Faraj , Abdulhussein Zina Jawad , Al-Jabry Saad Manee , Alabid Musaab Abdulrasool 
Published: November 2024

Background: The problem of mycotoxins in wheat crops can lead to health issues for humans and animals. It is very important to find safe and efficient methods to prevent the growth of toxin-producing fungi or reduce and remove them from food to prevent their risks to humans, animals, and agricultural crops. The paper focuses on finding environmentally friendly materials to protect wheat grains from toxin-secreting fungi, particularly Aspergillus niger, reducing the production of aflatoxin B1.Methods: Eight fungal genera were isolated from wheat grain samples, and the most frequent fungus was A. niger. Plant extracts were used to reduce the growth of A. niger, and the culture media was used to test the ability of A. niger fungus isolates to produce aflatoxin toxins. The media was also used to sow wheat grains for the purpose of isolating the fungi associated with them, growing the fungi, and purifying it. The culture media was poured into sterile petri dishes with a diameter of 9 cm, with three replicates for each concentration, and the comparison treatment in which an untreated food media was used. Results: The paper found that A. niger was the frequent fungus among the isolated fungal genera in wheat grain samples. The plant extracts, particularly citrullus extract, showed a clear effect in inhibiting the growth of A. niger and reduced the AFB1 toxin that it produces. The paper recommends continuous examination of stored wheat grains to determine the amount of fungal contamination and to ensure good storage conditions before receiving the grains in grain stores.Conclusion: The paper aimed to detect fungi associated with local and imported wheat grains stored in the stores of Al-Muthanna Governorate and the possibility of reducing them by using some plant extracts. Keywords: Citrullus; Phylex; Aspergillus niger; AFB1
The Relationship of CXCL12 with Sperm Agglutination and Anti-Sperm Antibody Among Infertile Men
Authors: Oleiwi Fahad Dhamin , J. Mohammed Mousa , Ali Chatin I. 
Published: November 2024

Background: It is known that structural elements like the ‘blood-testis barrier’ (BTB) and local & systemic tolerance mechanisms help to maintain testicular homeostasis, which shields germ cells and mature sperms from immune attack. Any damage to this barrier could result in immune attack and may cause infertility.Methods: The most important goal of this study was to find the vital connection between male infertility and immunological factors (CXCL12 and anti-sperm antibody). During the months of October 2021 and July 2022, a total of 144 samples were taken from patients who sought to have infertility tested. Semen and blood samples were taken from the subjects to assess seminal plasma CXCL12 levels and the prevalence of anti-sperm antibody (ASA) in their serum. Alternatively, research on sperm function parameters, sperm agglutination risk, and its relationship to ASA and CXCL12.Result: statistically there was no ‘significant’ difference in CXCL12 levels across study groups in this investigation. The prevalence of sperm agglutination was 23.61% across all patients under study, the majority of whom had asthenozoospermia. In contrast, 60% of the samples from ASA-positive patients were agglutinated. The results revealed a relatively weak positive link that may call for additional research even if there was no significant statistical correlation in the relationship between CXCL12 and sperm functions and ASA.Conclusion: The findings of this study suggest that ASA affects seminal fluid parameters, which in turn affects male fertility, and that a weak, non-significant association between CXCL12 and sperm function could impair sperm function by reducing sperm motility. Sperm agglutination could be a sign of immunological infertility. ASA can impact male fertility in a number of ways. Others had to do with sperm agglutination. Keywords: CXCL12; Anti-sperm antibody; ASA; Sperm agglutination; Sperm function
Effects of seed coating with (titanium dioxide and selenium) nanoparticles on fenugreek (Trigonella foenum graecum L.) plant growth and antioxidant activity
Authors: Al-Shammeryi Wasan H. Mazeil 
Published: November 2024

Background: The fenugreek plant is a kind of herbaceous plant that resembles a clover. It is extensively used as a spice and condiment, as well as a medicinal plant. Studying the effect of seed coating on fenugreek is an important step to learn about the effects it imprints on plant growth and its antioxidant activity when its seeds are coated by nanoparticles of titanium and selenium. Methods: To study the effects of seed coating with Nanoparticles of Titanium dioxide at concentrations of (0, 100, 200, 300, and 400) ppm and selenium at concentrations of (0, 20, 40, 60, and 80) ppm on shoot length, number of leaves per plant, carbohydrate %, protein %, and antioxidant activity peroxidase %, single and two way interaction on fenugreek’s growth is taken into account. Treatments are designed as a properly randomized factorial experiment (5×5×3), with three replicates in a totally randomized design.Results: It was revealed that a single application of the previously mentioned ingredients had a significant impact on fenugreek growth and antioxidant activity, especially at high concentrations.Conclusions: Plant physiological properties were favorably influenced by seed coating with (Titanium dioxide and Selenium) nanoparticles. Based on the results of TiO2 NPs’ effect on seed germination and early seedling growth, it is possible that NPs aided in seed water absorption, increased seed ability to absorb and utilize efficiently, and activated and promoted hydrolytic enzymes in the seed antioxidant system.Keywords: Titanium dioxide; Selenium; Nanoparticles; Antioxidant activity; Fenugreek
Computational assessment of targeting angiotensin-converting enzyme for hypertension management: A structure-based virtual screening approach
Authors: Alkathiri Ahmad Salah 
Published: November 2024

Background: Hypertension is a growing public health concern globally. The angiotensin-converting enzyme (ACE) is an enzyme that cleaves the carboxy-terminal His-Leu dipeptide from angiotensin I, yielding the potent vasopressor octapeptide, angiotensin II. ACE inhibitors are a primary treatment option for hypertension, heart failure, and myocardial infarction. However, the use of synthetic ACE inhibitors has been linked to a number of side effects. Therefore, the development of novel and safe ACE inhibitors is a need of time.Methods: This study used a computational screening of a library of known compounds with anti-inflammatory activities against the active site of ACE using the PyRx-Python 0.8 tool to find more potent ACE inhibitors with less or no side effects. The physicochemical properties of the anti-inflammatory compounds were obtained from the Life Chemicals website.Result: The five hits, specifically F3398-2114, F0193-0245, F0163-0089, F0193-0237, and F0302-0060, exhibited notable interactions within the ACE binding pocket and demonstrated greater binding energy compared to the control compound, Lisinopril. All of these compounds displayed favorable physicochemical characteristics and aligned to Lipinski's rule.Conclusion: The compounds F3398-2114, F0193-0245, F0163-0089, F0193-0237, and F0302-0060 have the potential to be used as ACE inhibitors; however, further experimental validation is required to optimize them as ACE inhibitors.Keywords: Hypertension; Angiotensin-converting enzyme; Heart failure; Lipinski's rule
Association of Celiac Disease and H. pylori Infection with ATG5 Polymorphism and Interleukin-33
Authors: Atrooz Omar , Mumtaz Sura , Abood Ahmed , Atrooz Ansam 
Published: November 2024

Background: Celiac disease (CD) is an inflammatory small intestine autoimmune disease. The study aims to investigate the association and the detection of ATG5 polymorphism between celiac disease (CD) and H. pylori infection, and the association with Interleukin-33 (IL-33).Methods: The study groups included patients from Iraq, ages 4 to 35. Two primary patient groups were created: sixteen had positive H. pylori and celiac disease, and thirty had positive CD and negative H. pylori disease. The levels of tissue transglutaminase IgA (TTG IgA), H. pylori IgG, and IL-33 were measured using the ELISA method. The primers were amplified using PCR.Results: With celiac disease, the patient group's TTG IgA levels increased dramatically. The test also showed significant variations (P=0.054) in the H. pylori IgG levels between the patient and control groups. The H. pylori seropositivity test showed a statistically significant difference (p ≤0.033) between seropositive and seronegative individuals, while the patient group's IL-33 levels did not significantly differ (P ≤0.299) from the control group.Conclusions: Our results showed that CD is more common in women and occurs in the age range of 24-35 years. It also showed that the mutant variant of ATG5 is associated with CD, and the significance of H. pylori IgG serum levels in the patient group may indicate that the bacteria involved in CD. Furthermore, H. pylori infection is more strongly linked to serum IL-33 levels than CD.Keywords: Celiac disease; H. pylori; PCR; IL-33; ATG5 polymorphism
A Virtual Insight into Bioactive Compounds of Ginger as Potential Inhibitors of NEK7 for Cancer Management
Authors: Aloqbi Akram Ahmed 
Published: November 2024

Background: NIMA-related kinase 7 (NEK7) is a serine/threonine kinase that is required for cell cycle progression, particularly mitotic spindle formation and cytokinesis, and has been linked to various cancers. Furthermore, NEK7 modulates the NLRP3 inflammasome, which activates Caspase-1 and causes cell pyroptosis. A growing body of studies indicates that ginger has the ability to prevent and treat a variety of health issues.Methods: In this study, 383 distinct bioactive compounds were extracted from the LOTUS database from Zingiber officinale and were screened against NEK7 protein using the PyRx 0.8 software. The top six hits' physicochemical characteristics, molecular descriptors, and natural product-likeness ratings were assessed using the LOTUS database.Results: The compounds LTS0101379, LTS0022317, TS0094590, LTS0231343, LTS0085481, and LTS0110340 showed high affinity for NEK7, forming interactions with critical amino acid residues. Notably, these compounds had multiple interactions with amino acid residues similar to those of the control compound Dabrafenib. Furthermore, the molecular properties and descriptors of these compounds indicated favorable drug-like properties.Conclusion: These findings highlight the potential of these compounds as NEK7 inhibitors for cancer management, though additional experimental validation is required.Keywords: NEK7; Cancer; Zingiber officinale; Virtual screening; Drug-likeness
In Vitro Synergistic Activity of Ceftazidime-Avibactam and Aztreonam against New Delhi Metallo-β-Lactamase-Producing Clinical Enterobacterales Isolates
Authors: AlAnazi Naif Fahd , Marie Mohammad , Somily Ali Mohammad , Binkhamis Khalifa , Arshad Muhammad , Alfuraydi Akram A. , Absar Muhammad 
Published: November 2024

Background: Carbapenemase-producing-Enterobacterales (CPE) infections are on the rise and associated with increased morbidity and mortality, due to a limited number of therapeutic options. The goal of this study was to assess the synergistic activity of ceftazidime-avibactam (CZA) and aztreonam (ATM) combination against phenotypically and genetically characterized blaMBL-producing Enterobacterales.Methods: In this study, forty (n=40) non-repeat, CPE clinical isolates, including: n=35 Klebsiella pneumoniae, n=2 each of Escherichia coli and Klebsiella oxytoca, and n=1 Enterobacter cloacae isolates were identified and susceptibilities were assessed using the Vitek-II compact (bioMérieux, Inc., France) and Microscan Walkaway (Beckman Coulter) systems. Genotypic analysis of clinically relevant carbapenemases was performed by using Xpert-Carba-R assay on GeneXpert (Cepheid, USA). The minimum inhibitory concentrations (MICs) and synergy testing of CZA and ATM, were tested by the Etest fixed ratio method (bioMérieux, Inc., France). The fractional inhibitory concentration index (FICI) was calculated for each antibiotic.Results: Our results showed that 97.5% of blaMBL-producing Enterobacterales isolates were susceptible to an in-vitro CZA + ATM combination regimen. The fractional inhibitory concentration index (FICI) ranged from 0.001 to 1.001. Among the tested CPE isolates, Synergy was observed in 36/40 (90%), an additive effect was observed in 5% (n=2)’ while two (5%) isolates showed indifference. There was no antagonism observed in our study.Conclusion: Our study exhibited a potent activity of CZA and ATM combination synergy against clinical CPE metallo- β-lactamase producers. More extensive studies involving a variety of Gram-negative pathogens with different resistance mechanisms are required to determine the efficacy of this combination regimen.Keywords: Synergy; Aztreonam; Ceftazidime-Avibactam; Carbapenem-Resistant Enterobacterales; Etest
Role of heavy metals pollution on emergence of antibiotic co-resistance in E. coli isolates
Authors: Kadhim Aseel AbdulRazaq , Mohammed Melad Khalaf , Jabbar Ahmed Darweesh 
Published: November 2024

Background: Heavy metal pollution in water is one of the most serious environmental problems. These findings have prompted the idea that metal-exposed bacteria may have altered resistance to antibiotics. This study was performed to investigate the presence of heavy metal resistance genes (HMRGs) in Escherichia coli.Methods: HMRGs and antibiotic resistance of 100 E. coli from sewage and fresh water were detected by PCR. Minimum inhibitory concentrations (MICs) of heavy metals were determined by the broth micro dilution method. Antibiotic discs used to detect multidrug resistance, was recovered and assembled using third-generation sequencing.Results: The frequency of different HMRGs in E. coli ranged from 1–67%., while prevalence of ESBL genes ranged 5-17% in freshwater isolates, and 54- 96% in swage isolates. MICs of heavy metals for E. coli ranged widely from ≤8.0-800 mg/L. Moreover, HMRGs (PbRT, cadD arsB, PcoA, czrc, and chrA) were found to be significantly associated with one or more ARGs ( tetA, blaTEM, blaSHV and blaCTX) (P < 0.05).Conclusion: In conclusion, HMRGs were widely present in E. coli isolated Dujla river and sewage water were significantly associated with DRGs . It is remarkable that the coexistence of HMRGs, DRGs and ARGs confer co-resistance to heavy metals, and antibiotics.Keywords: Heavy metals; Antibiotic resistance
Cultivar Mixtures as Part of Integrated Protection of Winter Barley from Leaf Diseases and Abiotic Stresses
Authors: Yakhnik Yana , Volkova Galina , Danilova Anastasia , Kutumov Kirill 
Published: November 2024

Background: Currently, cultivar mixtures are being extensively used in agriculture worldwide. The current research study aimed to identify the optimal proportion of cultivars with different resistance to pathogens to reduce the severity of leaf diseases in mixed cultivars against net blotch and leaf rust of barley.Methods: Leaf rust severity was assessed using a modified Cobb scale, and net blotch severity with the E. E. Geshele scale. Disease severity was averaged from triplicate measurements on 25–30 plants. After harvest, 1000 seeds per plot were weighed. Differences were evaluated using the Fisher test (α = 0.05), and correlations via the Chaddock scale in Statistica 13.3.Results: A high degree of inverse correlation was established between the proportion of resistant cultivars and the severity by both diseases r= – 0.93/– 0.99. The optimal mixture combination of a resistant and susceptible cultivar against net blotch was recorded as 1S:4R, and leaf rust as 1S:3R and 1S:4R.Conclusion: In mixed barley crops, a strong inverse correlation was found between the proportion of resistant cultivars and disease severity (r = –0.93/–0.99). Disease severity correlated directly with humidity and temperature in spring, with an inverse correlation for net blotch in June. Using mixed cultivars enhances crop resistance to abiotic and biotic stresses, making it suitable for eco-friendly farming.Keywords: Abiotic factors; Barley; Mixture of cultivars; Puccinia hordei; Pyrenophora teres
СМЫСЛОВАЯ ДОМИНАНТА «ПУТЕШЕСТВИЕ» В РОМАНЕ ДЖ. ДЖОЙСА «УЛИСС»: ПРОБЛЕМАТИКА СОПОСТАВИТЕЛЬНОГО АНАЛИЗА
Authors: Д.А. Кононов 
Published: November 2024

Статья посвящена анализу репрезентации смысловой доми-нанты «путешествие» в романе Дж. Джойса «Улисс» с точки зрения теории регуля-тивности. Предпринята попытка применить теорию регулятивности к крупной прозе потока сознания и на основе этого применения установить существенные для текстообразования связи между смысловой доминантой «путешествие» и смысловыми доминантами «отец» и «сын», занимающими центральное положе-ние в пространстве романа. В статье приводятся основные регулятивы, с помо-щью которых реализуется смысловая доминанта «путешествие». Кроме этого, предложен ряд критериев, позволяющих анализировать разные проявления этой смысловой доминанты в тексте романа.
This article contains the analysis of dominant concept JOURNEY rep-resentation in the novel “Ulysses” by James Joyce. The theory of regulativity has been applied to the large piece of stream of consciousness prose. This theory application allowed to determine specifying connections between the dominant concept JOURNEY and dominant concepts FATHER and SON that are substantial for text formation. The article provides the main regulatives which represent the dominant concept JOURNEY and criteria which can be applied for the analysis of different representa-tions of this dominant concept in the novel.