bluuebunny/crossref_metadata_2025_split huggingface dataset:
Volumetric optoacoustic tomography enables non-invasive in vivo characterization of impaired heart function in hypoxic conditions
Authors: Ivankovic Ivana , Deán-Ben Xose Luis , Lin Hsiao-Chun Amy , Zhang Zuwen , Trautz Benjamin , Petry Andreas , Görlach Agnes , Razansky Daniel 
Published: June 2019

AbstractExposure to chronic hypoxia results in pulmonary hypertension characterized by increased vascular resistance and pulmonary vascular remodeling, changes in functional parameters of the pulmonary vasculature, and right ventricular hypertrophy, which can eventually lead to right heart failure. The underlying mechanisms of hypoxia-induced pulmonary hypertension have still not been fully elucidated while no curative treatment is currently available. Commonly employed pre-clinical analytic methods are largely limited to invasive studies interfering with cardiac tissue or otherwise ex vivo functional studies and histopathology. In this work, we suggest volumetric optoacoustic tomography (VOT) for non-invasive assessment of heart function in response to chronic hypoxia. Mice exposed for 3 consecutive weeks to normoxia or chronic hypoxia were imaged in vivo with heart perfusion tracked by VOT using indocyanide green contrast agent at high temporal (100 Hz) and spatial (200 µm) resolutions in 3D. Unequivocal difference in the pulmonary transit time was revealed between the hypoxic and normoxic conditions concomitant with the presence of pulmonary vascular remodeling within hypoxic models. Furthermore, a beat-to-beat analysis of the volumetric image data enabled identifying and characterizing arrhythmic events in mice exposed to chronic hypoxia. The newly introduced non-invasive methodology for analysis of impaired pulmonary vasculature and heart function under chronic hypoxic exposure provides important inputs into development of early diagnosis and treatment strategies in pulmonary hypertension.
A semi-continuum model of saturation overshoot in one dimensional unsaturated porous media flow
Authors: Kmec Jakub , Fürst Tomáš , Vodák Rostislav , Šír Miloslav 
Published: June 2019

AbstractA semi-continuum model for fluid flow in saturated-unsaturated porous medium in one spatial dimension is presented. The model is based on well-established physics, measurable parameters and material characteristics. The porous material is characterized by porosity, intrinsic permeability, main wetting and draining branches of the retention curve, and the saturation dependence of the relative permeability. The fluid is characterized by its density and dynamic viscosity. The only physics involved is the mass balance of fluid in porous media together with the Darcy-Buckingham Law for fluid flow in unsaturated porous media. The model is a cellular automaton based on the Macro Modified Invasion Percolation concept of dividing the porous medium into blocks which are not infinitesimal and are assumed to retain the characteristics of a porous medium. The cellular automaton repeats three successive rules: saturation update in each block, pressure update in each block, and flux update between neighboring blocks. The model tracks the evolution of the relative saturation, the fluid capillary pressure, and the fluid flux. The model is shown to reproduce qualitatively and quantitatively all features of one dimensional saturation overshoot behavior reported in the literature.
Cellular morphological changes detected by laser scanning in vivo confocal microscopy associated with clinical outcome in fungal keratitis
Authors: Chidambaram Jaya D. , Prajna Namperumalsamy V. , Palepu Srikanthi , Lanjewar Shruti , Shah Manisha , Elakkiya Shanmugam , Lalitha Prajna , Macleod David , Burton Matthew J. 
Published: June 2019

AbstractHRT3 in vivo confocal microscopy (IVCM) images may indicate clinical outcome, but few studies have analysed this in fungal keratitis (FK). Adults with FK (diameter ≥3 mm) presenting to Aravind Eye Hospital, India from 2012-3 were enrolled prospectively. IVCM was performed at baseline, days 7, 14 and 21 post-enrolment (+/− 3 days where possible). Specific morphologies were identified in IVCM images by a grader masked to microbiology and clinical outcome (defined as good: healed/improving, or poor: enlarged ulcer, perforation or transplant/glue). Associations with final visit outcome assessed using logistic regression. 143 FK participants were enrolled; 87 had good outcome, 56 had poor outcome. Poor outcomes were associated with stellate interconnected cellular processes with no visible nuclei (OR 2.28, 95% CI: 1.03–5.06, p = 0.043) in baseline IVCM images, and fungal filaments (OR 6.48, 95% CI:2.50–16.78, p < 0.001) or honeycomb distribution of inflammatory cells (OR 5.24, 95% CI: 1.44–19.06, p = 0.012) in final visit images. Fungal filaments (OR 3.61, 95% CI:1.64–7.95, p = 0.001), stromal dendritiform cells (OR 2.88, 95% CI:1.17–7.11, p = 0.022), or stellate cellular processes with no visible nuclei (OR 2.09, 95% CI:1.14–3.82, p = 0.017) were associated with poor outcome if not in baseline but present in final visit images. IVCM can reveal morphological changes associated with clinical outcome.
Genetic analyses of early-onset Alzheimer’s disease using next generation sequencing
Authors: Giau Vo Van , Bagyinszky Eva , Yang Young Soon , Youn Young Chul , An Seong Soo A. , Kim Sang Yun 
Published: June 2019

AbstractAlzheimer’s disease (AD) is the most common type of neurodegenerative dementia, but the cause of AD remained poorly understood. Many mutations in the amyloid precursor protein (APP) and presenilin 1 and 2 (PSEN1 and PSEN2) have been reported as the pathogenic causes of early-onset AD (EOAD), which accounts for up to 5% of all AD cases. In this study, we screened familiar/de novo EOAD (n = 67) samples by next-generation sequencing (NGS) of a 50-gene panel, which included causative and possible pathogenic variants linked to neurodegenerative disorders. Remarkably, three missense mutations in PSEN1 (T119I, G209A, and G417A) and one known variant in PSEN2 (H169N) were discovered in 6% of the cases. Additionally, 67 missense mutations in susceptibility genes for late-onset AD were identified, which may be involved in cholesterol transport, inflammatory response, and β-amyloid modulation. We identified 70 additional novel and missense variants in other genes, such as MAPT, GRN, CSF1R, and PRNP, related to neurodegenerative diseases, which may represent overlapping clinical and neuropathological features with AD. Extensive genetic screening of Korean patients with EOAD identified multiple rare variants with potential roles in AD pathogenesis. This study suggests that individuals diagnosed with AD should be screened for other neurodegenerative disease-associated genes. Our findings expand the classic set of genes involved in neurodegenerative pathogenesis, which should be screened for in clinical trials. Main limitation of this study was the absence of functional assessment for possibly and probably pathogenic variants. Additional issues were that we could not perform studies on copy number variants, and we could not verify the segregation of mutations.
Immunostaining in whole-mount lipid-cleared peripheral nerves and dorsal root ganglia after neuropathy in mice
Authors: Bernal L. , Cisneros E. , García-Magro N. , Roza C. 
Published: June 2019

AbstractImmunohistochemical characterization of primary afferent fibers (intact or after nerve damage) is traditionally performed in thin sections from dorsal root ganglia (DRGs) or in teased fibers, as light scattering in whole-mounts compromises visualization. These procedures are time-consuming, require specific equipment and advanced experimental skills. Lipid-clearing techniques are increasing in popularity, but they have never been used for the peripheral nervous system. We established a modified, inexpensive clearing method based on lipid-removal protocols to make transparent peripheral nerve tissue (inCLARITY). We compared retrograde-labeling and free-floating immunostaining with cryo-sections. Confocal microscopy on whole-mount transparent DRGs showed neurons marked with retrograde tracers applied to experimental neuromas (Retrobeads, Fluoro-ruby, Fluoro-emerald, DiI, and Fluoro-gold). After immunostaining with calcitonin gene-related peptide (peptidergic) or isolectin IB4 (non-peptidergic), nociceptors were visualized. Immunostaining in transparent whole-mount nerves allows simultaneous evaluation of the axotomized branches containing the neuroma and neighboring intact branches as they can be mounted preserving their anatomical disposition and fiber integrity. The goal of our study was to optimize CLARITY for its application in peripheral nerve tissues. The protocol is compatible with the use of retrograde tracers and improves immunostaining outcomes when compared to classical cryo-sectioning, as lack of lipids maximizes antibody penetration within the tissue.
Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue
Authors: Guzmán-Delgado Nancy Elena , Carranza-Torres Irma Edith , García-Davis Sara , Rivera Gildardo , Morán-Martínez Javier , Betancourt-Martínez Nadia Denys , Groothuis G. M. M. , de Graaf I. A. M. , Carranza-Rosales Pilar 
Published: June 2019

AbstractAmoebiasis is a parasitic disease that causes thousands of deaths every year, its adverse effects and resistance to conventional treatments have led to the search of new treatment options, as well as the development of novel screening methods. In this work, we implemented a 3D model of intestine and liver slices from hamsters that were infected ex vivo with virulent E. histolytica trophozoites. Results show preserved histology in both uninfected tissues as well as ulcerations, destruction of the epithelial cells, and inflammatory reaction in intestine slices and formation of micro abscesses, and the presence of amoebae in the sinusoidal spaces and in the interior of central veins in liver slices. The three chemically synthetized compounds T-001, T-011, and T-016, which act as amoebicides in vitro, were active in both infected tissues, as they decreased the number of trophozoites, and provoked death by disintegration of the amoeba, similar to metronidazole. However, compound T-011 induced signs of cytotoxicity to liver slices. Our results suggest that ex vivo cultures of precision-cut intestinal and liver slices represent a reliable 3D approach to evaluate novel amoebicidal compounds, and to simultaneously detect their toxicity, while reducing the number of experimental animals commonly required by other model systems.
Impacts of changes in vegetation on saturated hydraulic conductivity of soil in subtropical forests
Authors: Hao Mingzhuo , Zhang Jinchi , Meng Miaojing , Chen Han Y. H. , Guo Xiaoping , Liu Shenglong , Ye Lixin 
Published: June 2019

AbstractSaturated hydraulic conductivity (Ks) is one of the most important soil properties that determines water flow behavior in terrestrial ecosystems. However, the Ks of forest soils is difficult to predict due to multiple interactions, such as anthropological and geomorphic processes. In this study, we examined the impacts of vegetation type on Ks and associated mechanisms. We found that Ks differed with vegetation type and soil depth, and the impact of vegetation type on Ks was dependent on soil depth. Ks did not differ among vegetation types at soil depths of 0–10 and 20–30 cm, but was significantly lower in managed forest types (mixed evergreen broad-leaved and coniferous forests, bamboo forests, and tea gardens) than native evergreen broadleaf forests at a depth of 10–20 cm. Boosted regression tree analysis indicated that total porosity, non-capillary porosity, and macro water-stable aggregates were the primary factors that influenced Ks. Our results suggested that vegetation type was a key factor that influences hydraulic properties in subtropical forest soils through the alteration of soil properties, such as porosity and macro water-stable aggregates.
Patients with III and IV category of the Bethesda System under levothyroxine non-suppressive therapy have a lower rate of thyroid malignancy
Authors: Kaliszewski Krzysztof , Diakowska Dorota , Wojtczak Beata , Sutkowski Krzysztof , Knychalski Bartłomiej , Forkasiewicz Zdzisław 
Published: June 2019

AbstractThyroid nodules (TNs) assigned to the Bethesda System categories III and IV include numerous clinical characteristics, which increase or decrease the risk of malignancy. However, there are very few data regarding the influence of TSH non-suppressive thyroid hormone therapy (NSTHT) on the risk of malignancy in patients in the aforementioned categories. We assessed the number of patients with thyroid nodules assigned to categories III and IV who take TSH NSTHT and if thyroid hormone therapy is associated with a rate of malignancy. We retrospectively analyzed the medical records of 4,716 individuals and selected 532 (11.28%) patients with Bethesda System category III and IV thyroid nodules. All participants underwent surgery, and histopathological verification was obtained in all cases. In all, 33.1% of individuals with category III and IV thyroid nodules took TSH NSTHT. In patients with category III nodules, application of NSTHT was associated with a lower rate of thyroid cancer (TC), though this observation was not significant (OR = 0.55, p = 0.381). In patients with category IV nodules, we demonstrated a significantly lower rate of TC when NSTHT was applied (OR = 0.44, p = 0.005). In conclusion, the prevalence of patients with Bethesda System category III and IV thyroid nodules who take NSTHT is high. TSH NSTHT significantly decreases a rate of malignancy in category IV, but not category III patients.
Optimal monitor positioning and camera rotation angle for mirror image: overcoming reverse alignment during laparoscopic colorectal surgery
Authors: Miura Susumu , Oshikiri Taro , Miura Yukiko , Takiguchi Gosuke , Takase Nobuhisa , Hasegawa Hiroshi , Yamamoto Masashi , Kanaji Shingo , Matsuda Yoshiko , Yamashita Kimihiro , Matsuda Takeru , Nakamura Tetsu , Suzuki Satoshi , Kakeji Yoshihiro 
Published: June 2019

AbstractMirror image is one of the most difficult situations that the assistant surgeon encounters in laparoscopic colorectal surgery. The aim of the present study was to investigate whether task performance with mirror images improves by changing the position of the monitor and the rotation angle of the camera. Twenty-four surgeons performed the task under different conditions: Coaxial image (C), Mirror image (M), Mirror image + Monitor on the left side of participants (M + Mon), Mirror image + Camera rotated 90 degrees to the right (M + Cam), and Mirror image + Monitor on the left side + Camera rotated to the right (M + Mon + Cam) in a training box. The outcome measure was the mean time for completing the task. The mean time for completing the task, in decreasing order, was M (111.4 ± 58.9 seconds) > M + Mon (70.5 ± 29.4 seconds) > M + Cam (47.1 ± 17.1 seconds) > M + Mon + Cam (33.4 ± 10.3 seconds) > C (20.5 ± 3.5 seconds). (multivariable analysis of variance (MANOVA), p = 7.9 × 10−7) Task performance with mirror images improved by changing the monitor positioning and camera rotation angle. This novel method is a simple way to overcome mirror image in laparoscopic colorectal surgery.
Wearable 1 V operating thin-film transistors with solution-processed metal-oxide semiconductor and dielectric films fabricated by deep ultra-violet photo annealing at low temperature
Authors: Yu Byoung-Soo , Jeon Jun-Young , Kang Byeong-Cheol , Lee Woobin , Kim Yong-Hoon , Ha Tae-Jun 
Published: June 2019

AbstractAmorphous metal-oxide semiconductors (AOSs) such as indium-gallium-zinc-oxide (IGZO) as an active channel have attracted substantial interests with regard to high-performance thin-film transistors (TFTs). Recently, intensive and extensive studies of flexible and/or wearable AOS-based TFTs fabricated by solution-process have been reported for emerging approaches based on device configuration and fabrication process. However, several challenges pertaining to practical and effective solution-process technologies remain to be resolved before low-power consuming AOS-based TFTs for wearable electronics can be realized. In this paper, we investigate the non-thermal annealing processes for sol-gel based metal-oxide semiconductor and dielectric films fabricated by deep ultraviolet (DUV) photo and microwave annealing at low temperature, compared to the conventional thermal annealing at high temperature. A comprehensive investigation including a comparative analysis of the effects of DUV photo and microwave annealing on the degree of metal-oxide-metal networks in amorphous IGZO and high-dielectric-constant (high-k) aluminum oxide (Al2O3) films and device performance of IGZO-TFTs in a comparison with conventional thermal annealing at 400 °C was conducted. We also demonstrate the feasibility of wearable IGZO-TFTs with Al2O3 dielectrics on solution-processed polyimide films exhibiting a high on/off current ratio of 5 × 104 and field effect mobility up to 1.5 cm2/V-s operating at 1 V. In order to reduce the health risk and power consumption during the operation of wearable electronics, the operating voltage of IGZO-TFTs fabricated by non-thermal annealing at low temperature was set below ~1 V. The mechanical stability of wearable IGZO-TFTs fabricated by an all-solution-process except metal electrodes, against cyclic bending tests with diverse radius of curvatures in real-time was investigated. Highly stable and robust flexible IGZO-TFTs without passivation films were achieved even under continuous flexing with a curvature radius of 12 mm.
The social dynamics of lung cancer talk on Twitter, Facebook and Macmillan.org.uk
Authors: Taylor Joanna , Pagliari Claudia 
Published: June 2019

AbstractPeople with lung cancer and others affected by the condition are using social media to share information and support, but little is known about how these behaviours vary between different platforms. To investigate this, we extracted posts from Twitter (using relevant hashtags), the Lung Cancer Support Group on Facebook and the Macmillan.org.uk lung cancer discussion forum for a single month. Interaction Process Analysis revealed that all three platforms were used more for giving than seeking information, opinion or suggestions. However, interaction types (including sentiment) varied between platforms, reflecting their digital architectures, user-base and inclusion of a moderator. For example, a higher percentage of information-seeking and sentiment marked the Macmillan.org.uk, compared with Twitter and the Facebook Group. Further analysis of the messages using a four-dimensional typology of social support revealed that emotional and informational support types were most prevalent on the Macmillan.org.uk forum, closely followed by the Facebook Group. Contrary to expectations, Twitter posts showed the most companionship support, reflecting the use of hashtags as user-generated signals of community belonging and interests. Qualitative analysis revealed an unanticipated sub-category of spiritual support, which featured uniquely in the Lung Cancer Support Group on Facebook. There was little evidence of trolling or stigma, although some users remarked that lung cancer was unfairly resourced compared with other cancers. These findings provide new insights about how people affected by lung cancer use social media and begin to elucidate the value of different platforms as channels for patient engagement and support, or as potential research data sources.
Test–Retest Reliability of Relative Fundamental Frequency and Conventional Acoustic, Aerodynamic, and Perceptual Measures in Individuals With Healthy Voices
Authors: Park Yeonggwang , Stepp Cara E. 
Published: June 2019

Purpose
Recent studies have shown that an acoustic measure, relative fundamental frequency (RFF), has potential for the assessment of excessive laryngeal tension and vocal effort associated with functional and neurological voice disorders. This study presents an analysis of the test–retest reliability of RFF in individuals with healthy voices and a comparison of reliability between RFF and conventional measures of voice.

Method
Acoustic and aerodynamic measurements and Consensus Auditory–Perceptual Evaluation of Voice (CAPE-V) were performed on 28 individuals with healthy voices on 5 consecutive days. Participants produced RFF stimuli, a sustained /ɑ/, and a reading passage to allow for extraction of acoustic measures and CAPE-V ratings; /pa/ trains were produced to allow for extraction of aerodynamic measures.

Results
Moderate reliabilities (intraclass correlation coefficient [ICC] = .64–.71) were found for RFF values. Mean vocal fundamental frequency, smoothed cepstral peak prominence, shimmer, harmonics-to-noise ratio, and mean airflow rate exhibited good-to-excellent reliabilities (ICC = .76–.99). ICCs for jitter and phonation threshold pressure were moderately reliable (ICC = .67–.74). ICCs for subglottal pressure estimates and all CAPE-V parameters showed poor reliabilities (ICC = .31–.58).

Conclusion
RFF has comparable reliability to conventional measures of voice. This expands the potential for clinical application of RFF.

Supplemental Material

https://doi.org/10.23641/asha.8233376
Reflection geometries in absorbed and unabsorbed AGN
Authors: Panagiotou C. , Walter R. 
Published: June 2019

Context. The hard X-ray emission of active galactic nuclei (AGN), and in particular, the reflection component, is shaped by the innermost and outer regions of the galactic nucleus.
Aims. Our main goal is to investigate the variation of the Compton hump amongst a population of sources and correlate it with other spectral properties to constrain the source geometry.
Methods. We studied the NuSTAR hard X-ray spectra of a sample of 83 AGN and performed a detailed spectral analysis of each of them. Based on their spectral shape, we divided the sample into five categories and also studied their stacked spectra.
Results. We found a stronger reflection in mildly obscured sources, which verifies the results reported in previous works. In addition, the reflection behaviour, and probably origin, varies with absorption. The accretion disc seems to be the main reflector in unabsorbed sources. A clumpy torus seems to produce most of the reflection in obscured sources. The filling factor of the clouds surrounding the active nucleus is a key parameter that drives the appearance of AGN. Finally, we found that the Fe line and the Compton hump are roughly correlated, as expected.
Psychokardiologie – aktuelle Leitlinien und klinische Realität
Authors: Herrmann-Lingen Christoph 
Published: June 2019

AbstractIn the last decades, psychocardiological knowledge has contributed a lot to the understanding of psychosocial factors involved in the pathogenesis, course, and treatment of cardiovascular diseases. Therefore, psychological, social, and behavioral factors should be detected and addressed early on in order to prevent cardiovascular disease and/or its progression. On the other hand, cardiovascular disease is an important stressor which can lead to psychological comorbidities and thus deteriorate prognosis and quality of life. During therapy a clear, consistent, and empathic communication between professionals and patients is of importance. Up to now there is not much evidence as to the effectiveness of psychotherapeutic interventions or antidepressant medication for cardiac patients with mental comorbidities. Beyond psychosomatic basic care, combined with physical activity, severely ill patients may require multimodal inpatient care in specialized psychosomatic or psychocardiological units and continuous support with lifestyle change.