bluuebunny/crossref_metadata_2025_split huggingface dataset:
Community-based interventions for prevention of Type 2 diabetes in low- and middle-income countries: a systematic review
Authors: Gyawali Bishal , Bloch Joakim , Vaidya Abhinav , Kallestrup Per 
Published: September 2018

Abstract
Type 2 diabetes is an increasing burden in low- and middle-income countries (LMICs). Knowledge of effective prevention programs in LMICs is thus important. The aim of this review was to establish an overview of studies evaluating the effectiveness of community-based interventions for prevention of Type 2 diabetes in LMICs. A literature review with searches in the databases using MEDLINE in Ovid (Ovid MEDLINE® In-Process & Other Non-Indexed Citations, Ovid MEDLINE(R) Daily, Ovid MEDLINE and Version (R); Embase; PsycINFO; Global Health; and Google Scholar) between 1 January 2000 and 31 December 2015 was conducted. Only 10 studies that met our selection criteria were included; 3 were randomized controlled trials, 2 non-randomized controlled trials and 5 were pre-and post-intervention studies. About 9 of 10 studies reported significant reduction in both the glycated hemoglobin (HbA1c) and fasting blood glucose (FBG) levels as a result of the intervention. A majority of the studies included multicomponent interventions such as education and behavioral encompassing both individual and group work, and included health education, nutrition education, nutrition counseling, exercise and promoting physical activity, psychosocial approaches and lifestyle modification. The interventions were delivered by community health workers, volunteers, social workers, community nutritionists and community nurses. Comparisons between studies, however, was not possible due to substantial heterogeneity in study design. This review contributes to the current literature on community-based interventions for prevention of Type 2 diabetes in LMICs, acknowledging the community-based approach can be effective in prevention and control of Type 2 diabetes. Due to the heterogeneity across study designs, outcomes and in terms of variation and duration of interventions, only limited conclusions can be drawn about the effectiveness of interventions. More evidence from randomized controlled trials on culturally tailored, community-based interventions is needed to compare findings and test implementation in practice.
Adherence to components of Health Promoting Schools in schools of Bengaluru, India
Authors: Periyasamy Sugavanesh , Krishnappa Pushpanjali , Renuka Piddennavar 
Published: September 2018

AbstractSchools provide a crucial platform for health promotion as the school years are a vital stage in one’s life, where lifelong general and oral health-related behaviours are developed and established. The components of Health Promoting Schools (HPSs) suggested by World Health Organization provide guidance for facilitating health promotion within this setting. This study aimed to assess the adherence to the components of HPSs amongst schools in Bengaluru, India utilizing a comprehensive tool developed for the purpose. A cross-sectional survey was conducted among randomly selected 61 schools. Data were collected through structured interviews with the head of the schools, direct observation of school premises and verification of records. The significant findings of the study were that 80.3% of the schools had proper ventilation and separate washrooms for boys and girls, 83.6 and 88.5% of the schools had natural light and adequate water supply correspondingly. Only 39.3% of the schools had washrooms that were cleaned daily, and 55.7% of the schools were in proximity to business that sold tobacco products. Oral health education was not integrated into the curriculum in 39.3% of the schools, and 29.5% of the schools had no playgrounds. The study tool appeared to be sensitive in identifying the finer components of HPSs, indicating the lack of strict adherence to the components of HPSs in Bengaluru. This information can be utilized to design appropriate interventions at micro, meso and macro level to strengthen the capacity of schools for the attainment of health promotion.
Individual-level determinants of waterpipe smoking demand in four Eastern-Mediterranean countries
Authors: Salloum Ramzi G , Nakkash Rima , Abu-Rmeileh Niveen M E , Hamadeh Randah R , Darawad Muhammad W , Kheirallah Khalid A , Al-Farsi Yahya , Yusufali Afzalhussein , Thomas Justin , Mostafa Aya , Salama Mohamed , El Kadi Lama , Alzyoud Sukaina , Al-Sheyab Nihaya , Thrasher James F 
Published: September 2018

Abstract
The prevalence of waterpipe tobacco smoking in the Eastern Mediterranean Region is at alarmingly high levels, especially among young people. The objective of this research was to evaluate the preferences of young adult waterpipe smokers with respect to potential individual-level determinants of waterpipe smoking using discrete choice experiment methodology. Participants were young adult university students (18–29 years) who were ever waterpipe smokers, recruited from universities across four Eastern Mediterranean countries: Jordan, Oman, Palestine and the United Arab Emirates. The Internet-based discrete choice experiment, with 6 × 3 × 2 block design, evaluated preferences for choices of waterpipe smoking sessions, presented on hypothetical waterpipe café menus. Participants evaluated nine choice sets, each with five fruit-flavored options, a tobacco flavored option (non-flavored), and an opt-out option. Choices also varied based on nicotine content (0.0% vs. 0.05% vs. 0.5%) and price (low vs. high). Participants were randomized to receive menus with either a pictorial + text health-warning message or no message (between-subjects attribute). Multinomial logit regression models evaluated the influence of these attributes on waterpipe smoking choices.
Across all four samples (n = 1859), participants preferred fruit-flavored varieties to tobacco flavor, lower nicotine content and lower prices. Exposure to the health warning did not significantly predict likelihood to opt-out. Flavor accounted for 81.4% of waterpipe smoking decisions.
Limiting the use of fruit flavors in waterpipe tobacco, in addition to accurate nicotine content labeling and higher pricing may be effective at curbing the demand for waterpipe smoking among young adults.
Inclusion of ecosystem information in US fish stock assessments suggests progress toward ecosystem-based fisheries management
Authors: Marshall Kristin N , Koehn Laura E , Levin Phillip S , Essington Timothy E , Jensen Olaf P 
Published: September 2018

Abstract
The appetite for ecosystem-based fisheries management (EBFM) approaches has grown, but the perception persists that implementation is slow. Here, we synthesize progress toward implementing EBFM in the United States through one potential avenue: expanding fish stock assessments to include ecosystem considerations and interactions between species, fleets, and sectors. We reviewed over 200 stock assessments and assessed how the stock assessment reports included information about system influences on the assessed stock. Our goals were to quantify whether and how assessments incorporated broader system-level considerations, and to explore factors that might contribute to the use of system-level information. Interactions among fishing fleets (technical interactions) were more commonly included than biophysical interactions (species, habitat, climate). Interactions within the physical environment (habitat, climate) were included twice as often as interactions among species (predation). Many assessment reports included ecological interactions only as background or qualitative considerations, rather than incorporating them in the assessment model. Our analyses suggested that ecosystem characteristics are more likely to be included when the species was overfished (stock status), the assessment is conducted at a science centre with a longstanding stomach contents analysis program, and/or the species life history characteristics suggest it is likely to be influenced by the physical environment, habitat, or predation mortality (short-lived species, sessile benthic species, or low trophic-level species). Regional differences in stomach contents analysis programs may limit the inclusion of predation mortality in stock assessments, and more guidance is needed on best practices for the prioritization of when and how biophysical information should be considered. However, our results demonstrate that significant progress has been made to use best available science and data to expand single-species stock assessments, particularly when a broad definition of EBFM is applied.
Mathematical modelling of trastuzumab-induced immune response in an in vivo murine model of HER2+ breast cancer
Authors: Jarrett Angela M , Bloom Meghan J , Godfrey Wesley , Syed Anum K , Ekrut David A , Ehrlich Lauren I , Yankeelov Thomas E , Sorace Anna G 
Published: September 2018

Abstract
The goal of this study is to develop an integrated, mathematical–experimental approach for understanding the interactions between the immune system and the effects of trastuzumab on breast cancer that overexpresses the human epidermal growth factor receptor 2 (HER2+). A system of coupled, ordinary differential equations was constructed to describe the temporal changes in tumour growth, along with intratumoural changes in the immune response, vascularity, necrosis and hypoxia. The mathematical model is calibrated with serially acquired experimental data of tumour volume, vascularity, necrosis and hypoxia obtained from either imaging or histology from a murine model of HER2+ breast cancer. Sensitivity analysis shows that model components are sensitive for 12 of 13 parameters, but accounting for uncertainty in the parameter values, model simulations still agree with the experimental data. Given theinitial conditions, the mathematical model predicts an increase in the immune infiltrates over time in the treated animals. Immunofluorescent staining results are presented that validate this prediction by showing an increased co-staining of CD11c and F4/80 (proteins expressed by dendritic cells and/or macrophages) in the total tissue for the treated tumours compared to the controls ($p < 0.03$). We posit that the proposed mathematical–experimental approach can be used to elucidate driving interactions between the trastuzumab-induced responses in the tumour and the immune system that drive the stabilization of vasculature while simultaneously decreasing tumour growth—conclusions revealed by the mathematical model that were not deducible from the experimental data alone.
Integrity of clinical information in computerized order requisitions for diagnostic imaging
Authors: Lacson Ronilda , Laroya Romeo , Wang Aijia , Kapoor Neena , Glazer Daniel I , Shinagare Atul , Ip Ivan K , Malhotra Sameer , Hentel Keith , Khorasani Ramin 
Published: September 2018

Abstract

Objective
Assess information integrity (concordance and completeness of documented exam indications from the electronic health record [EHR] imaging order requisition, compared to EHR provider notes), and assess potential impact of indication inaccuracies on exam planning and interpretation.

Methods
This retrospective study, approved by the Institutional Review Board, was conducted at a tertiary academic medical center. There were 139 MRI lumbar spine (LS-MRI) and 176 CT abdomen/pelvis orders performed 4/1/2016-5/31/2016 randomly selected and reviewed by 4 radiologists for concordance and completeness of relevant exam indications in order requisitions compared to provider notes, and potential impact of indication inaccuracies on exam planning and interpretation. Forty each LS-MRI and CT abdomen/pelvis were re-reviewed to assess kappa agreement.

Results
Requisition indications were more likely to be incomplete (256/315, 81%) than discordant (133/315, 42%) compared to provider notes (p &lt; 0.0001). Potential impact of discrepancy between clinical information in requisitions and provider notes was higher for radiologist’s interpretation than for exam planning (135/315, 43%, vs 25/315, 8%, p &lt; 0.0001). Agreement among radiologists for concordance, completeness, and potential impact was moderate to strong (Kappa 0.66-0.89). Indications in EHR order requisitions are frequently incomplete or discordant compared to physician notes, potentially impacting imaging exam planning, interpretation and accurate diagnosis. Such inaccuracies could also diminish the relevance of clinical decision support alerts if based on information in order requisitions.

Conclusions
Improved availability of relevant documented clinical information within EHR imaging requisition is necessary for optimal exam planning and interpretation.
Too Expensive to Treat? Non-Treatment Decisions at the Margins of Viability
Authors: Flessas Tatiana , Jackson Emily 
Published: September 2018

Abstract
This article seeks to challenge the assumption that it is legitimate to consider the costs of premature babies’ future social and educational needs when deciding what treatment, if any, to provide in the neonatal intensive care unit (NICU) . It questions the elision that is made between the claim that a particular treatment is insufficiently cost-effective and the claim that a person will be a burden on the state in the future. It discusses a series of common misunderstandings about how treatment decisions are taken in the NICU and concludes by suggesting that the claim that premature babies are too expensive to treat may depend upon regarding a premature infant as if she were not yet a person, with rights and interests of her own.
Modulation of the microbiota by oral antibiotics treats immunoglobulin A nephropathy in humanized mice
Authors: Chemouny Jonathan M , Gleeson Patrick J , Abbad Lilia , Lauriero Gabriella , Boedec Erwan , Le Roux Karine , Monot Céline , Bredel Maxime , Bex-Coudrat Julie , Sannier Aurélie , Daugas Eric , Vrtovsnik Francois , Gesualdo Loreto , Leclerc Marion , Berthelot Laureline , Ben Mkaddem Sanae , Lepage Patricia , Monteiro Renato C 
Published: September 2018

Abstract

Background
Immunoglobulin A nephropathy (IgAN) is the most common primary glomerulonephritis worldwide. IgA is mainly produced by the gut-associated lymphoid tissue (GALT). Both experimental and clinical data suggest a role of the gut microbiota in this disease. We aimed to determine if an intervention targeting the gut microbiota could impact the development of disease in a humanized mouse model of IgAN, the α1KI-CD89Tg mice.

Methods
Four- and 12-week old mice were divided into two groups to receive either antibiotics or vehicle control. Faecal bacterial load and proteinuria were quantified both at the beginning and at the end of the experiment, when blood, kidneys and intestinal tissue were collected. Serum mouse immunoglobulin G (mIgG) and human immunoglobulin A1 (hIgA1)-containing complexes were quantified. Renal and intestinal tissue were analysed by optical microscopy after haematoxylin and eosin colouration and immunohistochemistry with anti-hIgA and anti-mouse CD11b antibodies.

Results
Antibiotic treatment efficiently depleted the faecal microbiota, impaired GALT architecture and impacted mouse IgA production. However, while hIgA1 and mIgG serum levels were unchanged, the antibiotic treatment markedly prevented hIgA1 mesangial deposition, glomerular inflammation and the development of proteinuria. This was associated with a significant decrease in circulating hIgA1–mIgG complexes. Notably, final faecal bacterial load strongly correlated with critical clinical and pathophysiological features of IgAN such as proteinuria and hIgA1–mIgG complexes. In addition, treatment with broad-spectrum antibiotics reverted established disease.

Conclusions
These data support an essential role of the gut microbiota in the generation of mucosa-derived nephrotoxic IgA1 and in IgAN development, opening new avenues for therapeutic approaches in this disease.
Withholding Perioperative Steroids in Patients Undergoing Transsphenoidal Resection for Pituitary Disease: Randomized Prospective Clinical Trial to Assess Safety
Authors: Sterl Karin , Thompson Bithika , Goss Charles W , Dacey Ralph G , Rich Keith M , Zipfel Gregory J , Chicoine Michael R , Kim Albert H , Silverstein Julie M 
Published: September 2018

Abstract

BACKGROUND
Perioperative steroid protocols for patients undergoing transsphenoidal surgery (TSS) for pituitary pathology vary by institution.

OBJECTIVE
To assess the safety of withholding glucocorticoids in patients undergoing TSS.

METHODS
Patients with an intact hypothalamic-pituitary-adrenal (HPA) axis undergoing TSS for a pituitary tumor at the same academic institution between 2012 and 2015 were randomized to either receive 100 mg of intravenous hydrocortisone followed by 0.5 mg of intravenous dexamethasone every 6 h for 4 doses (STER, n = 23) or to undergo surgery without steroids (NOSTER, n = 20). Postoperative cortisol levels were then used to determine the need for glucocorticoids after surgery. Data regarding postoperative cortisol levels, hospital stay length, and complications were collected.

RESULTS
Mean postoperative 8 am cortisol levels were higher in the NOSTER group compared to the STER group (745 ± 359 nmol/L and 386 ± 193 nmol/L, respectively, P = .001) and more patients were discharged on glucocorticoids in the STER group (42% vs 12%, P = .07). There was no difference in the incidence of postoperative complications, including hyperglycemia, diabetes insipidus, or permanent adrenal insufficiency. Permanent adrenal insufficiency occurred in 8% of patients.

CONCLUSION
Perioperative steroids can be safely withheld in patients with an intact HPA axis undergoing TSS. Although administration of perioperative glucocorticoids does not appear to increase the risk of complications, it may interfere with assessment of the HPA axis after surgery.
Low-cost detection of norovirus using paper-based cell-free systems and synbody-based viral enrichment
Authors: Ma Duo , Shen Luhui , Wu Kaiyue , Diehnelt Chris W , Green Alexander A 
Published: September 2018

AbstractNoroviruses are a primary cause of gastroenteritis and foodborne illness with cases that affect millions of people worldwide each year. Inexpensive tests for norovirus that do not require sophisticated laboratory equipment are important tools for ensuring that patients receive timely treatment and for containing outbreaks. Herein, we demonstrate a low-cost colorimetric assay that detects norovirus from clinical samples by combining paper-based cell-free transcription–translation systems, isothermal amplification and virus enrichment by synbodies. Using isothermal amplification and cell-free RNA sensing with toehold switches, we demonstrate that the assay enables detection of norovirus GII.4 Sydney from stool down to concentrations of 270 aM in reactions that can be directly read by eye. Furthermore, norovirus-binding synbodies and magnetic beads are used to concentrate the virus and provide a 1000-fold increase in assay sensitivity extending its detection limit to 270 zM. These results demonstrate the utility of paper-based cell-free diagnostic systems for identification of foodborne pathogens and provide a versatile diagnostic assay that can be applied to the concentration, amplification and detection of a broad range of infectious agents.
Advances in Gene Ontology Utilization Improve Statistical Power of Annotation Enrichment
Authors: Hinderer Eugene W. , Flight Robert M. , Dubey Rashmi , MacLeod James N. , Moseley Hunter N.B. 
Published: September 2018

AbstractGene-annotation enrichment is a common method for utilizing ontology-based annotations in these gene and gene-product centric knowledgebases. Effective utilization of these annotations requires inferring semantic linkages by tracing paths through the ontology through edges in the ontological graph, referred to as relations. However, some relations are semantically problematic with respect to scope, necessitating their omission lest erroneous term mappings occur. To address these issues, we present GOcats, a novel tool that organizes the Gene Ontology (GO) into subgraphs representing user-defined concepts, while ensuring that all appropriate relations are congruent with respect to scoping semantics. Here, we demonstrate the improvements in annotation enrichment by re-interpreting edges that would otherwise be omitted by traditional ancestor path-tracing methods.We demonstrate that GOcats’ unique handling of relations improves enrichment over conventional methods in the analysis of two different gene-expression datasets: a breast cancer microarray dataset and several horse cartilage development RNAseq datasets. With the breast cancer microarray dataset, we observed significant improvement (one-sided binomial test p-value=1.86E-25) in 182 of 217 significantly enriched GO terms identified from the conventional path traversal method when GOcats’ path traversal was used. We also found new significantly enriched terms using GOcats, whose biological relevancy has been experimentally demonstrated elsewhere. Likewise, on the horse RNAseq datasets, we observed a significant improvement in GO term enrichment when using GOcat’s path traversal: one-sided binomial test p-values range from 1.32E-03 to 2.58E-44.
Structural variants exhibit allelic heterogeneity and shape variation in complex traits
Authors: Chakraborty Mahul , Emerson J.J. , Macdonald Stuart J. , Long Anthony D. 
Published: September 2018

AbstractDespite extensive effort to reveal the genetic basis of complex phenotypic variation, studies typically explain only a fraction of trait heritability. It has been hypothesized that individually rare hidden structural variants (SVs) could account for a significant fraction of variation in complex traits. To investigate this hypothesis, we assembled 14Drosophila melanogastergenomes and systematically identified more than 20,000 euchromatic SVs, of which ∼40% are invisible to high specificity short read genotyping approaches. SVs are common inDrosophilagenes, with almost one third of diploid individuals harboring an SV in genes larger than 5kb, and nearly a quarter harboring multiple SVs in genes larger than 10kb. We show that SV alleles are rarer than amino acid polymorphisms, implying that they are more strongly deleterious. A number of functionally important genes harbor previously hidden structural variants that likely affect complex phenotypes (e.g.,Cyp6g1, Drsl5, Cyp28d1&2, InR, andGss1&2).Furthermore, SVs are overrepresented in quantitative trait locus candidate genes from eight Drosophila Synthetic Population Resource (DSPR) mapping experiments. We conclude that SVs are pervasive in genomes, are frequently present as heterogeneous allelic series, and can act as rare alleles of large effect.