bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dimers of glutaredoxin 2 as mitochondrial redox sensors in selenite-induced oxidative stress
Authors: Scalcon Valeria , Tonolo Federica , Folda Alessandra , Bindoli Alberto , Rigobello Maria Pia 
Published: May 2019

Abstract
Glutaredoxin 2 (Grx2) has been previously shown to link thioredoxin and glutathione systems receiving reducing equivalents by both thioredoxin reductase and glutathione. Grx2 catalyzes protein glutathionylation/de-glutathionylation and can coordinate an iron–sulfur cluster, forming inactive dimers stabilized by two molecules of glutathione. This protein is mainly located in the mitochondrial matrix, though other isoforms have been found in the cytosolic and nuclear cell compartments. In the present study, we have analyzed the monomeric and dimeric states of Grx2 under different redox conditions in HeLa cells, and sodium selenite was utilized as the principal oxidizing agent. After selenite treatment, an increased glutathione oxidation was associated to Grx2 monomerization and activation, specifically in the mitochondrial compartment. Interestingly, in mitochondria, a large decline of thioredoxin reductase activity was also observed concomitantly to Grx2 activity stimulation. In addition, Grx2 monomerization led to an increase free iron ions concentration in the mitochondrial matrix, induction of lipid peroxidation and decrease of the mitochondrial membrane potential, indicating that the disassembly of Grx2 dimer involved the release of the iron–sulfur cluster in the mitochondrial matrix. Moreover, sodium selenite-triggered lipid and protein oxidation was partially prevented by deferiprone, an iron chelator with mitochondriotropic properties, suggesting a role of the iron–sulfur cluster release in the observed impairment of mitochondrial functions. Thus, by sensing the overall cellular redox conditions, mitochondrial Grx2 dimers become active monomers upon oxidative stress induced by sodium selenite with the consequent release of the iron–sulfur cluster, leading to activation of the intrinsic apoptotic pathway.
Qingyihuaji formula reverses gemcitabine resistant human pancreatic cancer through regulate lncRNA AB209630/miR-373/EphB2-NANOG signals
Authors: Chen Peng , Wang Meiying , Wang Cuiping 
Published: May 2019

Abstract
To investigate the possible mechanism of Qingyihuaji formula (QYHJ) for reversing gemcitabine (GEM) resistant human pancreatic cancer. Cell proliferation, apoptosis, migration and invasion were detected in CFPAC-1 cells. Xenograft mice established with CFPAC-1 through subcutaneous on 33 immunodeficient nude mice and randomly divided into four groups: vehicle, GEM (35 mg/kg), QYHJ (40 g/kg), and GEM + QYHJ (35 mg/kg + 40 g/kg) groups for 28-day treatment. Tumor growth and the mRNA expression of lncRNA AB209630, miR373, EphB2, and NANOG evaluated in dissected tumor tissue by real-time PCR, the CD133+ cancer stem cells were isolated by flow cytometer, and the changes of the tumor sphere forming were measured. QYHJ, especially the combination of GEM and QYHJ, was significantly inhibited the cell proliferation and migration of CFPAC-1 in vitro in the indicated times. The combination of GEM and QYHJ also remarkably promoted the cell apoptosis of CFPAC-1. QYHJ treatment effectively blocked the tumor growth in nude mice. QYHJ, especially GEM + QYHJ treatment, was significantly increased the mRNA expression of lncRNA AB209630, significantly decreased the mRNA levels of miR373, EphB2 and NANOG, and markedly reduced the tumor sphere formation and the numbers of CD133+ stem cells. In addition, GEM alone treatment had no significant effect in the above biomarker changes. QYHJ could effectivly enhance the antihuman pancreatic tumor activity of GEM, which may be through inhibiting pancreatic cancer stem cell differentiation by lncRNA AB209630/miR-373/EphB2-NANOG signaling pathway.
Psychometric Evaluation of the Bilingual English–Spanish Assessment Sentence Repetition Task for Clinical Decision Making
Authors: Fitton Lisa , Hoge Rachel , Petscher Yaacov , Wood Carla 
Published: May 2019

Purpose

The purpose of this study was (a) to examine the underlying components or factor structure of the Bilingual English–Spanish Assessment (BESA;
Peña, Gutiérrez-Clellen, Iglesias, Goldstein, & Bedore, 2014
) sentence repetition task and (b) to examine the relationship between Spanish–English speaking children's sentence repetition and vocabulary performance.

Method
Participants were 291 Spanish–English speaking children in kindergarten and 1st grade. Item analyses were used to evaluate the underlying factor structure for each language version of the sentence repetition tasks of the BESA. The tasks were then examined in relation to a measure of English receptive vocabulary.

Results
Bifactor models, which include a single underlying general factor and multiple specific factors, provided the best overall model fit for both the Spanish and English versions of the task. There was no relation between children's overall Spanish sentence repetition performance and their English vocabulary. However, children's pronoun, noun phrase, and verb phrase item scores in Spanish significantly predicted their English vocabulary scores. For English sentence repetition, both children's overall performance and their specific performance on the noun phrase items were predictors of their English vocabulary scores. Follow-up analyses revealed that, for the purposes of clinical assessment, the BESA sentence repetition tasks can be considered essentially unidimensional, lending support to the current scoring structure of the test.

Conclusions
Study findings suggest that sentence repetition tasks can provide insight into Spanish–English speaking children's vocabulary skills in addition to their morphosyntactic skills when used on a broad research scale. From a clinical assessment perspective, results indicate that the sentence repetition task has strong internal validity and support to the use of this measure in clinical practice.
Use of Neonatal Lung Ultrasound for the Early Detection of Pneumothorax
Authors: Deng Bi-Ying , Li Ning , Wu Wen-Shen , He Xiao-Guang , Li Jin-Feng , Huang Tian-li , Li Yu-Chan , Jiang Shuang-lan 
Published: May 2019

Objective Pneumothorax (PTX) can be diagnosed using lung ultrasonography (LUS) in adult patients, but there are only a few reports of LUS in PTX diagnosis in neonates. The aim of the study was to assess the diagnostic accuracy for PTX.
Study Design This was a retrospective review study performed in our neonatal intensive care unit (level III) between June 2015 and June 2018. All eligible patients underwent an LUS scan before undergoing a chest X-ray (CXR), which was considered the reference standard. When a diagnosis of PTX was inconsistent between LUS and CXR, a chest computed tomography (CT) scan or chest drain was considered the gold standard.
Results Among 86 infants included in the study, 30 (34.9%) were diagnosed with PTX. In these 30 infants, 35 PTXs were detected by bedside LUS (five bilateral PTXs). Moreover, 11 infants with 14 PTXs were diagnosed only by LUS and were missed by CXR. Out of these 11 infants, 7 underwent a CT scan, whereas the remaining 4 underwent thoracentesis that confirmed PTX diagnosis.
Conclusion In neonates with PTX, LUS was more sensitive and specific for the early detection of PTX compared with CXR.
Is Foley Catheter Use during a Trial of Labor after Cesarean Associated with Uterine Rupture?
Authors: Katz Eriksen Jennifer L. , Chandrasekaran Suchitra , Delaney Shani S. 
Published: May 2019

Objective We sought to assess the safety of transcervical Foley catheter (TCF) placement for cervical ripening in women undergoing induction of labor (IOL) after prior cesarean by evaluating the risk of uterine rupture.
Study Design We performed a secondary analysis of the Maternal-Fetal Medicine Unit's Cesarean Section Registry, a prospective observational cohort study. We included women with a history of ≤2 low-transverse cesarean deliveries who underwent IOL at ≥24 weeks of gestational age with a live singleton fetus without major anomalies. We excluded those who received prostaglandins or laminaria. We performed multinomial logistic regression to calculate adjusted odds ratios (aORs) for uterine rupture and dehiscence. Relevant confounders included prior vaginal delivery, pregnancy-induced hypertension, chorioamnionitis, and cervical effacement and dilation on admission.
Results A total of 2,564 women were eligible. Unadjusted analysis demonstrated no increased risk of uterine rupture with TCF (1.9 vs. 0.9%; p = 0.10) but an increased risk of uterine dehiscence (1.9 vs. 0.6%; p = 0.02). After adjustment, TCF was not associated with an increased risk of uterine rupture (aOR: 2.02; 95% confidence interval [CI]: 0.71–5.78) or uterine scar dehiscence (aOR: 1.32; 95% CI: 0.37–4.72).
Conclusion Foley catheter is a safe tool for mechanical dilation in women undergoing IOL after prior cesarean.
Laser- and cryogenic probe-assisted NMR enables hypersensitive analysis of biomolecules at submicromolar concentration
Authors: Okuno Yusuke , Mecha Miranda F. , Yang Hanming , Zhu Lingchao , Fry Charles G. , Cavagnero Silvia 
Published: May 2019

Significance
Despite its prominent role in structural biology, NMR spectroscopy is hampered by extremely poor sensitivity. In this work, we develop a laser-assisted methodology, LC-photo-CIDNP, to enhance NMR sensitivity for the detection of amino acids and proteins in solution down to nanomolar concentration. Our method is significant because it enables detection of nanomolar concentrations of aromatic amino acids (Trp and Tyr), either in their free state or within proteins, in only a few seconds. Hence, the LC-photo-CIDNP approach renders data collection thousands of times faster than laser-free NMR experiments performed on higher-field spectrometers. Further, LC-photo-CIDNP is readily applicable to both 1D and 2D data collection mode. Rapid detection of proteins in solution at nanomolar concentration by NMR spectroscopy is unprecedented.
Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins
Authors: Caceres Paulo S. , Gravotta Diego , Zager Patrick J. , Dephoure Noah , Rodriguez-Boulan Enrique 
Published: May 2019

Significance
Epithelial cells perform critical protective, secretory, absorptive, and sensory functions, for which they require plasma membrane polarization into apical and basolateral domains. Impaired polarity causes cancer and developmental and degenerative disorders. Research on fundamental polarity mechanisms has been hindered by the paucity of model proteins and by the use of overexpression systems. Here, we introduce a high-throughput surface proteomics approach based on domain-selective biotinylation and quantitative mass spectrometry that provides candidate proteins to study polarity under normal expression levels. Using this approach, we described that clathrin adaptors mediate apical and basolateral distribution of surface proteins, expanding the traditional notion that clathrin adaptors mediate only basolateral polarity. Our results establish quantitative surface proteomics as a powerful tool to study epithelial polarity.
Cardiac myosin binding protein-C phosphorylation regulates the super-relaxed state of myosin
Authors: McNamara James W. , Singh Rohit R. , Sadayappan Sakthivel 
Published: May 2019

Significance
Cardiac myosin binding protein-C (cMyBP-C) is a sarcomeric protein closely linked to cardiac contractility. Importantly, although the phosphorylation status of cMyBP-C regulates the force and rate of cardiac contraction, the exact molecular mechanism(s) remain(s) unclear. Previously, loss of, or mutations in, cMyBP-C resulted in a loss of the inhibited super-relaxed state (SRX) of myosin, which explains the hypercontractile phenotype. Thus, we asked if cMyBP-C phosphorylation would increase contractile function by the release of myosin heads from SRX to a state more conducive to their interaction with actin. We found that a site-specific, phosphorylation-dependent interaction between cMyBP-C and myosin does modulate the fraction of SRX myosin that controls contractility.
Perfluorocarbon nanoparticle-mediated platelet inhibition promotes intratumoral infiltration of T cells and boosts immunotherapy
Authors: Zhou Zaigang , Zhang Baoli , Zai Wenjing , Kang Lin , Yuan Ahu , Hu Yiqiao , Wu Jinhui 
Published: May 2019

Significance
Trafficking of T cells to tumors is critical for the success of immunotherapy. The success against solid cancers is not as effective as desired owing to limited efficacy in delivering a higher frequency of T cells to the tumor microenvironment. At this time, no useful methods are available to enhance T-cell infiltration in tumors in a short time. New methods that could enhance T-cell trafficking in tumors are urgently in need. Up to now, the role of platelets in limiting T-cell infiltration in tumors is a neglected phenomenon. In our study, for the first time, we proved that perfluorocarbon nanoparticles could promote intratumoral infiltration of T cells via platelet inhibition, resulting in enhanced anti–PD-L1 immunotherapy.
Variable G protein determinants of GPCR coupling selectivity
Authors: Okashah Najeah , Wan Qingwen , Ghosh Soumadwip , Sandhu Manbir , Inoue Asuka , Vaidehi Nagarajan , Lambert Nevin A. 
Published: May 2019

Significance
G protein-coupled receptors (GPCRs) regulate a wide variety of important cellular processes and are targeted by a large fraction of approved drugs. GPCRs signal by activating heterotrimeric G proteins and must couple to a select subset of G proteins to produce appropriate intracellular responses. It is not known how GPCRs select G proteins, but it is generally accepted that the Gα subunit C terminus is the primary G protein determinant of coupling selectivity. We systematically studied coupling of GPCRs to four families of G proteins and chimeras with C terminal regions that were exchanged between families. We uncovered rules for coupling selectivity and found that different GPCRs can recognize different features of the same G protein for selective coupling.
Mitochondrial adaptations to high intensity interval training in older females and males
Authors: Chrøis Karoline Maise , Dohlmann Tine Lovsø , Søgaard Ditte , Hansen Camilla Vestergaard , Dela Flemming , Helge Jørn Wulff , Larsen Steen 
Published: May 2019

AbstractIntroduction: High intensity interval training (HIIT) has shown to be as effective as moderate intensity endurance training to improve metabolic health. However, the current knowledge on the effect of HIIT in older individuals is limited and it is uncertain whether the adaptations are sex specific. The aim was to investigate effects of HIIT on mitochondrial respiratory capacity and mitochondrial content in older females and males. Methods: Twenty‐two older sedentary males (n = 11) and females (n = 11) completed 6 weeks of supervised HIIT 3 days per week. The training consisted of 5 × 1 min cycling (124 ± 3% of max power output at session 2–6 and 135 ± 3% of max power output at session 7–20) interspersed by 1½ min recovery. Before the intervention and 72 h after last training session a muscle biopsy was obtained and mitochondrial respiratory capacity, citrate synthase activity and proteins involved in mitochondria metabolism were assessed. Furthermore, body composition and ⩒O2max were measured. Results: ⩒O2max increased and body fat percentage decreased after HIIT in both sexes (p < 0.05). In addition, CS activity and protein content of MnSOD and complex I‐V increased in both sexes. Coupled and uncoupled mitochondrial respiratory capacity increased only in males. Mitochondrial respiratory capacity normalised to CS activity (intrinsic mitochondrial respiratory capacity) did not change following HIIT. Conclusion: HIIT induces favourable adaptions in skeletal muscle in older subjects by increasing mitochondrial content, which may help to maintain muscle oxidative capacity and slow down the process of sarcopenia associated with ageing.
Management of the correlations of UltracoldBosons in triple wells
Authors: Dutta Sunayana , Tsatsos Marios C , Basu Saurabh , Lode Axel U J 
Published: May 2019

Abstract
Ultracold interacting atoms are an excellent tool to study correlation functions of many-body systems that are generally eluding detection and manipulation. Herein, we investigate the ground state of bosons in a tilted triple-well potential and characterize the many-body state by the eigenvalues of its reduced one-body density matrix and Glauber correlation functions. We unveil how the interplay between the interaction strength and the tilt can be used to control the number of correlated wells as well as the fragmentation, i.e. the number of macroscopic eigenvalues of the reduced one-body density matrix.
Describing many-body localized systems in thermal environments
Authors: Wu Ling-Na , Schnell Alexander , Tomasi Giuseppe De , Heyl Markus , Eckardt André 
Published: May 2019

Abstract
In this work we formulate an efficient method for the description of fully many-body localized systems in weak contact with thermal environments at temperature T. The key idea is to exploit the representation of the system in terms of quasi-local integrals of motion (l-bits) to efficiently derive the generator for the quantum master equation in Born–Markov approximation. We, moreover, show how to compute the steady state of this equation efficiently by using quantum-jump Monte-Carlo techniques as well as by deriving approximate kinetic equations of motion. As an example, we consider a one-dimensional disordered extended Hubbard model for spinless fermions, for which we derive the l-bit representation approximately by employing a recently proposed method valid in the limit of strong disorder and weak interactions. Coupling the system to a global thermal bath, we study the transport between two leads with different chemical potentials at both of its ends. We find that the temperature-dependent current is captured by an interaction-dependent version of Mott’s law for variable range hopping, where transport is enhanced/lowered depending on whether the interactions are attractive or repulsive, respectively. We interpret these results in terms of spatio-energetic correlations between the l-bits.
The Complex Torque Coefficient Applied in Subsynchronous Oscillation Caused by HVDC
Authors: Zhang Hanhua , Li Jiaqi , Wen Jun , Zhang Shuang , Tian Bei 
Published: May 2019

Abstract
Subsynchronous oscillation is an unstable state in power system, which may damage rotors of turbine generators and break the steady operation of the power system. In China, some accidents of subsynchronous oscillations had happened in the power stations where there are HVDC projects nearby. So that it is necessary to study the Subsynchronous oscillation caused by HVDC. After analysis of the principle of subsynchronous oscillation, the mathematical calculation model of the system containing HVDC is built and analysed in method of complex torque coefficient. And the simulation model is established in PSCAD/EMTDC to prove the validation of the method of complex torque coefficient used for the system containing HVDC, which combines calculation and simulation of the method for the system.
Study on three-Study unbalanced control of low voltage phase distribution network
Authors: Zhang Chu , Fu Zhouxing , Ye Huanfei , Niu Dan 
Published: May 2019

Abstract
Three-phase unbalance is one of the power quality problems. For the power quality problem caused by the three-phase unbalance phenomenon on the load side in the low-voltage distribution network, combined with the characteristics of the three-phase four-wire system of the distribution network in China, the three-phase unbalance adjustment is studied. The device control strategy is selected, and the three-phase four-bridge arm topology and the three-phase current real-time detection method based on instantaneous reactive power theory are selected. The experimental model is built by MATLAB/Simulink to verify the feasibility of the method.
Fuzzy force control based on grasped objects stiffness of tendon-driven underactuated prosthetic hand system
Authors: Huanxin Luo , Hua Deng 
Published: May 2019

Abstract
The design of an fuzzy force based on grasped objects stiffness method for tendon-driven underactuated prosthetic hand system are addressed in this study. First, a mathematical model of tendon-driven underactuated prosthetic hand is developed for force control. Next, fuzzy force based on grasped objects stiffness was discussed to improve the control performance. Finally, the proposed control strategy for underactuated prosthetic hand is simulated to six different stiffness objects. The results shown that the controller is suit for prosthetic hand grasp objects with different stiffness. The simulation results verify the effectiveness of the force control method based on grasped objects stiffness.
Speed Estimation of Asynchronous Motor Based on Model Reference Adaptive Method and On-line Identification of Rotor Time Constant
Authors: Jun TONG , Lei SHI , Li-juan LIU , Chen-yue Fan 
Published: May 2019

Abstract
In this paper, the principle of model reference adaptive method (MRAS) is analysed and studied. Aiming at the shortcomings of installing speed sensor to collect the speed signal and feedback to the control system, a method for speed estimation and parameter identification of asynchronous motor without speed sensor is presented, And taking the model reference adaptive method (MRAS) as the main line, the Popov super stability theory is used to derive and analyse the adaptive law. Finally, the proposed method is verified by MATLAB software and hardware circuit experiments.
Feasibility Study of Point Type Equipment for Detecting Train Transponder System
Authors: Chen Fei , Lv Jingyang 
Published: May 2019

Abstract
In the development of railway transportation, safety has always been the most concerned issue. Balise is the core content of high-speed railway train control system, it is one of the most basic unit of information storage and transmission, control and status information necessary for the train. It is affected by many factors in the process of operation, so it is unavoidable to have problems of malfunction. At present, staff are required to operate the transponder to repair or update message information. In order to meet the needs of daily maintenance, the scheme of reading and writing operation of transponder by dynamic train detection is proposed and its feasibility is analyzed. This paper designs the software part of the mobile detection system by analyzing the working status information of different versions of transponders and tests it. The test results show that the dynamic reading and writing operation scheme is feasible.
The Location of Electric Vehicle Charging Station Based on TLBO Optimization Algorithm
Authors: Jinlei Hu , Yunlian Sun , Junwei Yu , Jue Lu , Qiwu Zou , Xinlin Xie , Bin Fu 
Published: May 2019

Abstract
With the large-scale application of electric vehicles, the construction of electric vehicle charging stations has become a prerequisite for urgent needs. In this paper, TLBO optimization algorithm is proposed. The optimal model of the location and the constant volume of the charging station is established based on the weighted distance and minimum distance from the user to the charging station. Based on the layout of the district network in the planning area, the construction plan of the electric vehicle charging station in the planning area is studied. Through a case study of a planning area, the results show that the TLBO optimization algorithm is applied to the location and constant volume of electric vehicle charging stations, with accurate calculation, rapid convergence, and good global optimization performance.