bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developmental Regression and Epilepsy of Infancy with Migrating Focal Seizures Caused by TBCD Mutation: A Case Report and Review of the Literature
Authors: Zhang Yunjian , Zhang Linmei , Zhou Shuizhen 
Published: September 2019

AbstractMicrotubule dynamics plays a crucial role in neuronal development and function. Variants in the tubulin cofactor D (TBCD) gene, which encodes one of the five co-chaperones required for assembly and disassembly of α/β-tubulin heterodimers, may lead to neurodevelopmental disorders. We aimed to study the clinical, electroencephalographic, and imaging features of a male patient with TBCD variants, and to provide a detailed review of the previously reported cases of TBCD-related neurological disorders. The patient presented with early-onset developmental regression, secondary microcephaly, epilepsy of infancy with migrating focal seizures, hypotonia, and brain atrophy with thin corpus callosum on brain magnetic resonance imaging. Genetic analyses of the family members revealed a compound heterozygous variant of c.230A > G (p.H77R) in the proband and deletion of exons 28 to 39 of TBCD, which has not been previously reported and was inherited from his carrier parents. Epilepsy of the patient was refractory to numerous antiepileptic drugs. The review of 33 previously reported patients revealed that the age at the onset was very early, and all the patients had presentations during the first year of life. This case report provides insight regarding the clinical features and genetic etiology of TBCD-related tubulinopathy. Identification of phenotypes and genotypes in patients may help in early diagnosis and appropriate genetic counseling.
Healing Process Following Application of Set or Fresh Mineral Trioxide Aggregate as a Root-End Filling Material
Authors: Habibi Mehdi , Ghoddusi Jamileh , Habibi Ataollah , Mohtasham Nooshin 
Published: September 2019

ABSTRACTObjectives: An unsuccessful attempt to reach the apical area or to place the retrograde material is a major difficulty in periradicular surgery. The aim of this study was to compare the histological evaluation of the healing process following an orthograde versus a retrograde application of mineral trioxide aggregate (MTA) as a root-end filling material during apical surgery on cats’ teeth in order to find out whether orthograde placement of MTA before surgery can be used instead of retrograde placement during surgery. Methods: In this experimental study, 24 canine teeth in 12 mature and healthy cats were filled with either MTA or gutta-percha in an orthograde manner. Two weeks later, the teeth with MTA were surgically exposed and resected to the set-MTA within the canals. The teeth previously filled by gutta-percha were also surgically exposed, and retrograde cavities were prepared at the root ends and filled with fresh-MTA. After 8 weeks, the animals were euthanized by vital perfusion. Six-micron histological slices were prepared from samples, stained by Hematoxylin & Eosin, and histologically studied by means of a light microscope. The collected data was analyzed by the Chi-square and the T-test. Results: One of the samples in the fresh-MTA group was omitted during processing because of inappropriate sectioning. In the set-MTA group, 5 out of 12 showed chronic abscess, while in the fresh-MTA group, 2 out of 11 were discovered to have chronic abscess; however, no significant difference was observed (P>.05). Hard tissue healing (cementum, bone, cementum + bone formation) in the set-MTA and fresh-MTA groups were 7 out of 12 and 9 out of 11, respectively. While healing seemed more likely to occur in the fresh-MTA group, the difference was statistically insignificant (P>.05). The magnitude of bone, cementum, or bone and cementum formation showed slight differences between the two groups; however, the figures failed to show any marked differences (P>.05). Conclusions: Orthograde placement of MTA could be used as an obturation material before surgery. In this way, after root-end resection, there would be no need for root-end preparation and filling procedures. (Eur J Dent 2011;5:19-23)
Effects of Hydrogen Peroxide on Dental Unit Biofilms and Treatment Water Contamination
Authors: Lin Shih-Ming , Svoboda Kathy K.H. , Giletto Anthony , Seibert Jeff , Puttaiah Raghunath 
Published: September 2019

ABSTRACTObjectives: To study effects of various concentrations of hydrogen peroxide on mature waterline biofilms and in controlling planktonic (free-floating) organisms in simulated dental treatment water systems; and to study in vitro the effects of 2%, 3%, and 7% hydrogen peroxide on the removal of mature biofilms and inorganic compounds in dental waterlines. Methods: Four units of an automated dental unit water system simulation device was used for 12 weeks. All units were initially cleaned to control biofilms and inorganic deposits. H2O2 at concentrations of 1%, 2%, 3% was used weekly for periodic cleaning in three treatment group units (units 1, 2 & 3), with 0.05%, 0.15% and 0.25% H2O2 in municipal water used as irrigant respectively. The control unit (unit 4) did not have weekly cleanings and used municipal water as irrigant. Laser Scanning Confocal Microscopy and Scanning Electron Microscopy were used to study deposits on lines, and weekly heterotrophic plate counts done to study effluent water contamination. A 24 hour in vitro challenge test with 7%, 3% and 2% H2O2 on mature biofilms was conducted using harvested waterlines to study biofilm and inorganic deposit removal. Results: Heterotrophic plate counts of effluent water showed that the control unit reached contamination levels in excess of 400,000 CFU/mL while all treatment units showed contamination levels <500 CFU/mL through most of the 12 weeks. All treatment units showed varying levels of biofilm and inorganic deposit control in this short 12 week study. The in vitro challenge test showed although there was biofilm control, there was no eradication even when 7% H2O2 was used for 24 hours. Conclusions: 2% H2O2 used as a periodic cleaner, and diluted to 0.05% in municipal water for irrigation was beneficial in controlling biofilm and planktonic contamination in dental unit water systems. However, to remove well established biofilms, it may take more than 2 months when initial and multiple periodic cleanings are performed using H2O2. (Eur J Dent 2011;5:47-59)
Does Dental Insurance Make a Difference in Type of Service Received by Iranian Dentate Adults?
Authors: Bayat Fariborz , Murtomaa Heikki , Vehkalahti M Miira , Tala Heikki , Mautsch Walter 
Published: September 2019

ABSTRACTObjectives: To assess the relationship between insurance status and type of service received among dentate adults in a developing oral health care system. Methods: A cross-sectional survey based on phone interviews in Tehran, Iran. Four trained interviewers collected data using a structured questionnaire. Of 1,531 subjects answering the phone call, 224 were <18 years; of the remaining 1,307, 221 (17%) refused to participate, and 85 (6%) were excluded as edentate or reporting no dental visit, leaving 1,001 eligible subjects in the sample. The questionnaire covered insurance status, socio-demographics, frequency of tooth brushing, dental attendance as reasons for, and time since last dental visit, and dental service received then. Data analysis included the chi-square test and logistic regression. Results: Of the subjects, 71% had a dental insurance. Those with no insurance were more likely to report tooth extractions (OR=1.5) than those with an insurance coverage; for all other treatments no differences according to the insurance status appeared. Among the insured subjects, extractions were more likely for those reporting a problem-based dental visit (OR=6.0) or having a low level of education (OR=2.3). Conclusions: In Iran, with its developing oral health care system, dental insurance had only a minor impact on dental services reported. (Eur J Dent 2011;5:68-76)
Effect of Naturally Acidic Agents on Microhardness and Surface Micromorphology of Restorative Materials
Authors: Hengtrakool Chanothai , Kukiattrakoon Boonlert , Kedjarune-Leggat Ureporn 
Published: September 2019

ABSTRACTObjectives: This study investigated the titratable acidity and erosive potential of acidic agents on the microhardness and surface micromorphology of four restorative materials. Methods: Forty-seven discs of each restorative material; metal-reinforced glass ionomer cement (Ketac-S), resin-modified glass ionomer cement (Fuji II LC), resin composite (Filtek Z250) and amalgam (Valiant-Ph.D.), 12 mm in diameter and 2.5 mm in thickness, were divided into four groups (5 discs/group). Specimens were then immersed for 7 days into four storage media; deionized water (control), citrate buffer solution, green mango juice and pineapple juice. Microhardness testing before and after immersions was performed. Micromorphological changes were evaluated under a scanning electron microscope (SEM). Statistical significance among each group was analyzed using two-way repeated ANOVA and Tukey's tests. Results: The Fuji II LC and the Ketac-S showed the highest reduction in microhardness (P<.05). The Valiant-Ph.D. and the Filtek Z250 showed some minor changes over the period of 7 days. The mango juice produced the greatest degradation effect (P<.05). Conclusions: This study suggested that for restorations in patients who have tooth surface loss, materials selected should be considered. In terms of materials evaluated, amalgam and resin composite are the most suitable for restorations. (Eur J Dent 2011;5:89-100)
Bilateral Palatal Talon Cusps on Permanent Maxillary Lateral Incisors: A Case Report
Authors: Ozcelik Bahar , Atila Burcu 
Published: September 2019

ABSTRACTTalon cusp is a rare developmental extra cusp-like projection on the cingulum area of affected anterior teeth that may cause various functional and aesthetic problems. The present report describes a case of bilateral palatal talon cusps on permanent maxillary incisors and the treatment procedure to overcome the clinical problems associated with talon cusps. (Eur J Dent 2011;5:113-116)
The Effect of the Pre-Conditioning Step on the Shear Bond Strength of Nano-Filled Resin-Modified Glass-Ionomer to Dentin
Authors: El-Askary Farid S , Nassif Mohammed S 
Published: September 2019

ABSTRACTObjectives: The purpose of this study was to evaluate the effect of the pre-conditioning step using
different dentin conditioners on the shear bond strength (SBS) of the nano-filled resin-modified glass ionomer (RMGI) to dentin. Methods: Twenty-four human molars were used in this study. Subsequent to teeth sectioning in the mesio-distal direction and creation of the smear layer, the teeth were divided into six groups with and without the pre-conditioning step. Dentin surfaces were restored with RMGI cylinders and the specimens were stored in distilled water at 37ºC for 24 hours. SBS test were performed using a Universal Testing Machine at a crosshead speed of 0.5 mm/minute. Statistical analysis was performed using One-Way ANOVA followed by Duncan’s Multiple Range Test. RMGI/dentin interface was evaluated using SEM operated at 12 Kv at 2000X magnification. Results: 35% phosphoric acid and EDTA yielded significantly higher SBS (12.0±1.8 and 11.9±4.4 MPa, respectively), compared to all tested groups. In addition, 25% polyacrylic acid (8.6±3.1 MPa) reported significantly higher SBS than the nano-filled RMGI when applied according to manufacturer instructions (5.5±2.2 MPa). When nano-filled RMGI was applied without its nano-primer, or directly over 25% polyacrylic acid conditioned dentin exhibited 100% pre-test failure, and their SBS were expressed as 0.0±0.0 MPa. SEM revealed that the nano-primer was unable to decalcify and hybridize the dentin surface. Conclusions: Based on the results of the current study, the pre-conditioning step effectively improves the SBS of nano-filled RMGI to dentin. The self-adhesiveness of nano-filled RMGI was not proved in this study. (Eur J Dent 2011;5:150-156)
Surface Modifications on Aesthetically Restored Teeth following Home Bleaching with 16% Peroxide Carbamide
Authors: Bodanezi Augusto , Bittencourt Marcos Eugênio de , Bodanezi Rafaela Vanni , Zottis Tatiana , Munhoz Etiene Andrade , Carlini-Júnior Bruno 
Published: September 2019

ABSTRACTObjectives: This clinical study evaluated the effects of a highly concentrated home-bleaching agent on the surface morphology of aesthetically restored teeth. Methods: Specimens of human enamel and micro-hybrid composite resin were randomly adhered to the buccal side of right premolar and molar teeth of ten volunteers, who underwent a routine home-bleaching procedure with 16% carbamide peroxide on the upper jaw for 8 days. The mandibular unbleached specimens served as paired controls (n=10). Ultra-structural assessment of the sample was carried out by scanning electron microscopy, and the resulting images were blindly evaluated for topographical alterations. The differences between groups were calculated with the Mann-Whitney test adjusted at the 95% confidence interval. Results: The bleached enamel presented significantly more morphological changes than the control group. The aspect of resin composite exposed to the whitening substance was statistically similar to that observed in the corresponding control group (P<.05). Conclusions: The occurrence of surface topography changes during home bleaching of aesthetically restored teeth with 16% carbamide peroxide was restricted to enamel. (Eur J Dent 2011;5:157- 162)
Effect of Bleaching Gels on Surface Roughness of Nanofilled Composite Resins
Authors: Wang Linda , Francisconi Luciana Fávaro , Atta Maria Teresa , Santos Jean Rodrigo dos , Padre Natália Coelho Del , Gonini-Júnior Alcides , Fernandes Karen Barros Parron 
Published: September 2019

ABSTRACTObjectives: The aim of this study was to investigate the superficial texture of composite restorations after different bleaching protocols. Methods: Filtek Supreme (S), Filtek Z350 (F), and Grandio (G) were compared to Opallis (O) and Filtek Z250 (Z) (control microhybrid composites) and to bovine enamel using three different bleaching agents: 35% hydrogen peroxide Whiteness HP (WHP), 35% Whiteness HP MAXX (WMAXX) and 16% carbamide peroxide Whiteness Standard (WS). Six specimens from each composite were treated using each bleaching agent, according to the manufacturers’ instructions. Three random sites were measured for superficial roughness (Hommel Tester T 1000) weekly for each sample. Data were analyzed for each bleaching system using two-way ANOVA and Bonferroni tests at 5% significance level. Results: WHP treatment significantly altered the Filtek Supreme composite over time. When WMAXX was used, Grandio displayed the most significant alterations in surface roughness throughout the evaluation period, which was not observed for the other nanocomposites. Using WS, Filtek Z250 presented significant surface alterations over time, which was not seen in the nanofilled materials. Conclusions: Surface roughness alteration was material and time-dependent. The bleaching gels affected nanofilled and microhybrid composite resins. Enamel was the surface less affected by bleaching. (Eur J Dent 2011;5:173-179)
Effectiveness of Ozone with or without the Additional Use of Remineralizing Solution on Non-Cavitated Fissure Carious Lesions in Permanent Molars
Authors: Atabek Didem , Oztas Nurhan 
Published: September 2019

ABSTRACTObjectives: The aim of this study was to evaluate the efficiency of ozone alone and with a re-mineralizing solution following application on initial pit and fissure caries lesions in permanent molars. Methods: Forty children (9-12 years) having non-cavitated fissure caries lesions on bilateral 40 first permanent mandibular molar teeth were participated in the study. Patients were randomly allocated to 2 experimental groups consisting of 20 subjects. In the first group, ozone was applied once for 40 seconds to the assigned test teeth of each pair. In the second group, ozone was applied once for 40 seconds to the assigned test teeth of each pair with the use of re-mineralizing solution. Progression or improvement of the caries was assessed at baseline, immediately after treatment and at 1-,2-,3-, and 6 month follow-up by comparing the DIAGNOdent values, Clinical Severity Indexes, Oral Hygiene Scores. The results were analyzed statistically by using the Wilcoxon-Test for dependent samples in each group. When comparing different test groups (control and experimental groups) the Friedman S test followed by the Mann-Whitney U test was used. Results: A statistically significant difference was found between all of the control and experimental test lesions in each group (P<.001). However, there was no statistically significant difference between the ozone treated groups and those using the additional re-mineralizing solution (P>.001). Conclusions: Ozone treatment either alone or combined with a re-mineralizing solution was found to be effective for remineralization of initial fissure caries lesions. (Eur J Dent 2011;5:393-399)
Effect of C-factor and LED Curing Mode on Microleakage of Class V Resin Composite Restorations
Authors: Alomari Qasem Diefallah , Barrieshi-Nusair Kefah , Ali Mohammad 
Published: September 2019

ABSTRACTObjectives: To investigate the effects of cavity C-factor and LED curing mode on microleakage of class V resin composite restorations. Methods: Eighty extracted human maxillary premolars were divided into four groups. In groups I and II, V-shaped class V cavities (C-factor ≈ 1.5) were prepared while box-shaped class V cavities (high C-factor ≈ 4) were prepared in groups III and IV. All the cavities were prepared on the vestibular surfaces of the teeth. All the cavities were prepared by one operator with specific dimensions. The same adhesive system (Excite) and the same restorative composite (Tetric Ceram) were used in all the groups. The restorations in groups I and III were cured with the fast curing mode of LED curing light, while the restorations in groups II and IV were cured with the soft-start mode of the same curing light. The samples were thermocycled, immersed in a 0.5% basic fuchsin solution for 24 hours, and sectioned. The degree of dye penetration was measured quantitatively and then qualitatively at both enamel and dentin/cementum margins. Results: Quantitatively, there was no statistical difference in the degree of microleakage between the groups at the enamel margin (P>.05). At the dentin/cementum margin, group III showed significantly more dye penetration than the other groups (P<.001). Similarly, qualitative measurements of dye penetration showed that group III had higher microleakage at dentin/cementum margin than the remaining groups (I and III P<.001; II and III P<.01; III and IV P<.01). Paired sample statistics showed that microleakage at the dentin/cementum margin was not statistically different from enamel microleakage (P>.05), except for group III (P<.000). Conclusions: Fast curing mode of LED curing light resulted in more microleakage at the gingival floor of class V cavities with high C-factor. There was no difference in dye penetration between enamel and dentin margins of the cavities except when the fast curing mode was used with high C-factor cavities. Both quantitative and qualitative measurements of microleakage gave the same results at both enamel and dentin margins. (Eur J Dent 2011;5:400-408)
The Frequency and Distribution of
Idiopathic Osteosclerosis of the Jaw
Authors: Sisman Yildiray , Ertas Elif Tarim , Ertas Huseyin , Sekerci Ahmet Ercan 
Published: September 2019

ABSTRACTObjectives: To determine the prevalence of idiopathic osteosclerosis (IO) in the jaw by radiographic evaluation and to investigate the relationship between the findings in relation to age, gender, and localization. Methods: The study included 2,211 panoramic radiographs obtained from the patients (915 men and 1,296 women) whose ages ranged from 10-77 and who visited the Department of Oral Diagnosis and Radiology in the Faculty of Dentistry, Erciyes University between 2008 and 2009. Results: Of 2,211 patients, 135 patients (6.1%) had IO. The prevalence obtained in our study was in the range reported in the literature. IO was detected more often in mandible rather than the maxilla. In addition, mandibular molar localization was the most common localization, and most of the lesions were associated with root apices. Conclusions: In view of the findings, IO can be defined as developmental variations of normal bony architecture, which are unrelated to local stimuli. The lesions can arise at any age, any location with no sex predilection, and IO usually requires no treatment other than diagnosis. Because all these lesions were located in the jaw and could only is detected in panoramic evaluations, this indicates the importance of careful diagnostic evaluation of radiographies in dental examinations. (Eur J Dent 2011;5:409-414)
A Comparison of Three-Dimensional and Two-Dimensional Cephalometric Evaluations of Children with Cleft Lip and Palate
Authors: Tulunoglu Ozlem , Esenlik Elcin , Gulsen Ayse , Tulunoglu Ibrahim 
Published: September 2019

ABSTRACTObjectives: The aim of this retrospective study was to compare the consistency of orthodontic measurement performed on cephalometric films and 3D CT images of cleft lip and palate (CLP) patients. Methods: The study was conducted with 2D radiographs and 3D CT images of 9 boys and 6 girls aged 7-12 with CLP. 3D reconstructions were performed using MIMICS software. Results: Frontal analysis found statistical differences for all parameters except occlusal plane tilt (OcP-tilt) and McNamara analysis found statistical differences in 2D and 3D measurements for all parameters except ANS-Me and Co-Gn; Steiner analysis found statistical differences for all parameters except SND, SNB and Max1-SN. Intra-group variability in measurements was also very low for all parameters for both 2D and 3D images. Conclusions: Study results indicate significant differences between measurements taken from 2D and 3D images in patients with cleft lip and palate. (Eur J Dent 2011;5:451-458)
Influence of previous acid etching on interface morphology and bond strength of self-etching adhesive to cavosurface enamel
Authors: Lima Adriano Fonseca , Silva Vinícius Brito da , Soares Giulliana Panfiglio , Marchi Giselle Maria , Aguiar Flávio Henrique Baggio , Lovadino José Roberto 
Published: September 2019

ABSTRACTObjectives: The aim of this study was to evaluate the (1) bond strength of a etch-and-rinse and self-etching adhesive systems to cavosurface enamel, (2) influence of the previous acid etching with phosphoric acid 35% to the self-etching adhesive application on bond strength values, and (3) analysis of the cavosurface enamel morphology submitted to different types of conditioning, with the use of a scanning electronic microscope (SEM).Methods: Twenty four human third molars were sectioned on mesio-distal direction, resulting in two slices. The specimens were ground flat with 600-grit aluminum oxide papers, and were randomly divided into three groups: Group 1 (etch-and-rinse adhesive system (control group)), Group 2 (selfetching adhesive), and Group 3 (self-etching adhesive with previous 35% phosphoric acid-etching for 15 s). Four cylinders (0.75 mm of diameter, 1 mm height) were confectioned prior to the microshear test. Four samples for each group were prepared according the cavosurface enamel treatment and were analyzed in an SEM.Results: Group 3 had the highest values on bond strength to cavosurface enamel compared to the other two groups, which presented statistically similar values. The performance of acid etching before the application of the self-etching adhesive results in an etching pattern that is different than the other groups, favoring the adhesion to the cavosurface enamel.Conclusions: Acid etching increases the bond strength values of the self-etching adhesive to cavosurface enamel, promoting a conditioning pattern that favors the adhesion to this substrate. (Eur J Dent 2012;6:56-62)