bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accuracy of augmented reality navigated surgery for placement of zygomatic implants: a human cadaver study
Authors: Tabernée Heijtmeijer Sander , Glas Haye , Janssen Nard , Vosselman Nathalie , de Visscher Sebastiaan , Spijkervet Fred , Raghoebar Gerry , de Bree Remco , Rosenberg Antoine , Witjes Max , Kraeima Joep 
Published: December 2024

Purpose
Placement of zygomatic implants in the most optimal prosthetic position is considered challenging due to limited bone mass of the zygoma, limited visibility, length of the drilling path and proximity to critical anatomical structures. Augmented reality (AR) navigation can eliminate some of the disadvantages of surgical guides and conventional surgical navigation, while potentially improving accuracy. In this human cadaver study, we evaluated a developed AR navigation approach for placement of zygomatic implants after total maxillectomy.

Methods
The developed AR navigation interface connects a commercial navigation system with the Microsoft HoloLens. AR navigated surgery was performed to place 20 zygomatic implants using five human cadaver skulls after total maxillectomy. To determine accuracy, postoperative scans were virtually matched with preoperative three-dimensional virtual surgical planning, and distances in mm from entry-exit points and angular deviations were calculated as outcome measures. Results were compared with a previously conducted study in which zygomatic implants were positioned with 3D printed surgical guides.

Results
The mean entry point deviation was 2.43 ± 1.33 mm and a 3D angle deviation of 5.80 ± 4.12° (range 1.39–19.16°). The mean exit point deviation was 3.28 mm (±2.17). The abutment height deviation was on average 2.20 ± 1.35 mm. The accuracy of the abutment in the occlusal plane was 4.13 ± 2.53 mm. Surgical guides perform significantly better for the entry-point (P = 0.012) and 3D angle (P = 0.05); however, there is no significant difference in accuracy for the exit-point (P = 0.143) when using 3D printed drill guides or AR navigated surgery.

Conclusion
Despite the higher precision of surgical guides, AR navigation demonstrated acceptable accuracy, with potential for improvement and specialized applications. The study highlights the feasibility of AR navigation for zygomatic implant placement, offering an alternative to conventional methods.
Evaluation of the effect of a communication skills course on medical students’ attitude towards patient-centered care: a prospective study
Authors: Mohamed Khalid Gaffer , Almarabheh Amer , Almughamsi Asim Mukhideer , Atwa Hany , Shehata Mohamed Hany 
Published: December 2024

Background
This study aims to examine whether a communication skills course (Early Clinical Exposure–ECE) might support shaping medical students’ attitudes towards patient-centered care at Taibah University, Saudi Arabia. Attitudes were measured before and after the course using the Patient Practitioner Orientation Scale (PPOS), which assesses students’ orientation towards patient-centeredness.

Methods
This prospective observational study collected data at two points: the start of year two and the start of year four from the same cohort. The PPOS, divided into the sharing subscale (physician shares information, power, and control with the patient) and the caring subscale (physician considers the patient’s emotions, preferences, and expectations), was used to evaluate students’ attitudes. Mean scores were calculated, and independent sample t-tests were employed to assess statistical significance.

Results
Of the students targeted, 153 participated at year two (response rate = 93.5%) and 143 at year four (response rate = 89.5%). Significant progress was observed in the sharing subscale, with scores increasing from 3.23 ± 0.53 to 3.40 ± 0.60 (p = 0.015). However, the caring subscale exhibited a significant decline, with scores dropping from 4.06 ± 0.53 to 3.76 ± 0.68 (p < 0.001). As a result, there was no significant overall change in the total PPOS score (p = 0.282). Female students showed more progress in the sharing subscale than males.

Conclusions
The communication skills course might have had a positive impact on students’ attitudes towards sharing information in decision-making with patients, but notably, the medical students’ caring attitudes declined. This indicates a need for further emphasis on patient-centered communication, especially on the caring aspects, in the medical curriculum.
Genetic diversity of the Khorat snail-eating turtle (Malayemys khoratensis) in Thailand
Authors: Chaianunporn Thotsapol , Mokarat Monthakarn , Ruangsakunporn Apichaya , Walalite Tanapipat , Kutanan Wibhu , Lomthaisong Khemika , Chaianunporn Kanokporn , Keithmaleesatti Sarun 
Published: December 2024

Introduction
Malayemys khoratensis is a freshwater turtle species endemic to northeastern Thailand and Lao PDR. While M. khoratensis is relatively common and widespread in Thailand, its population size and demographic trends remain largely unknown, as direct population estimates are lacking. This species faces significant threats from consumption, exploitation, and trade. Therefore, this study aims to investigate the genetic diversity, population structure, and demographic trends of M. khoratensis in northeastern Thailand, using two mitochondrial DNA genes, Cyt b and ND4.

Method
We grouped the turtles based on three major river basins in northeastern Thailand: Mun, Chi, and Northeast Mekong to assess population structure and test the isolation-by-basin hypothesis. The Cyt b and ND4 genes of 49 M. khoratensis individuals across 15 provinces in Thailand were sequenced, and 15 sequences from a previous study of Ihlow et al. (2016) were included into the analyses. We constructed a haplotype network from the concatenated sequences and analyzed the population structure with the analysis of molecular variance (AMOVA). Additionally, we used a Bayesian Skyline plot (BSP) analysis to estimate the effective female population size and explore the demographic history of this species.

Results
Our analysis identified 13 unique haplotypes defined by 26 polymorphic sites from 64 samples. The total haplotype diversity for M. khoratensis was 0.819, while nucleotide diversity was 0.0031. The haplotype network and AMOVA revealed no distinct population structure among the river basins, suggesting gene flow across these regions. The BSP estimated a stable effective female population size of approximately 130,000 individuals since around 100,000 years ago (late Pleistocene).

Conclusion
Our findings indicated no significant population structure by river basin, which may be due to natural movement between basins or potential human-mediated translocations linked to the turtle trade. The demographic trend of M. khoratensis appears to have been stable over time. However, given the ongoing exploitation of this species, we recommend regular monitoring of population trends to support its long-term survival.
A single-center retrospective study of ectopic lymphoid tissues in idiopathic membranous nephropathy: clinical pathological characteristics and prognostic value
Authors: Zhang Jing , Chen Siyu , Zheng Haiying , Rao Siyi , Lin Yuanyuan , Wan Jianxin , Chen Yi 
Published: December 2024

Background
In recent years, ectopic lymphoid tissue (ELT) has been increasingly confirmed as a new biomarker for kidney injury or inflammation. However, there is insufficient research on the relationship between ELT grading and the progression of idiopathic membranous nephropathy (IMN).

Methods
A total of 147 patients with biopsy-proven IMN in our institution from March 2020 to June 2022 were classified into five grades based on the different distribution of lymphocyte subsets in renal tissue (G0: no B cells or T cells, G1: scattered B and T cells, G2: clustered B and T cells, G3: an aggregation region of B and T cells without a central network, G4: highly organized and formed zones of B and T cells with a central network of follicular dendritic cells and scattered macrophages), and were further divided into low-grade group (G0+G1), intermediate-grade group (G2) and high-grade group (G3+G4). The clinicopathological data, induction treatment response and prognosis among the three groups were analyzed and compared retrospectively.

Results
As the grading of ectopic lymphoid tissues increased, patients were older, with a higher prevalence of hypertension, a higher 24-h urinary protein level, lower baseline hemoglobin and estimated glomerular filtration rate (eGFR) levels, and more severe renal pathological damage. Logistic regression analysis showed that after 6 months of induction treatment, patients in the high-grade group were more likely to be in non-remission than those in the low-grade group (odds ratios [ORs] of the three adjusted models were 4.310, 4.239, and 5.088, respectively, P-values were 0.005, 0.006, and 0.001, respectively). Kaplan-Meier survival analysis indicated that patients in the intermediate- and high-grade groups had significantly lower renal cumulative survival rate than those in the low-grade group (P = 0.025). Univariate Cox analysis showed that the risk of adverse renal outcome was 3.662 times higher in the intermediate- and high-grade groups than in the low-grade group (95% confidence interval [CI] [1.078–12.435]; P = 0.037). Multivariate Cox analysis revealed that failure of remission at the first 6 months (hazard ratio [HR] = 5.769; 95% CI [1.854–17.950]; P = 0.002) remained an independent risk factor for poor renal outcome in patients with IMN.

Conclusions
Grading of renal ectopic lymphoid tissues correlates with disease activity and severity in IMN patients and can be used as an indicator to assess the risk of IMN progression.
Market Chain Analysis of Small Ruminant Value in Tahtay Adyabo District, Tigray Region, Ethiopia
Authors: Desta Zemeda , Zemedu Lemma , Tegegne Bosena 
Published: December 2024

The objective of this study was to examine market performance of small ruminant value chainin Tahtay Adyabo District. Multi-stage random sampling technique was used to select representative small ruminant producer kebeles and sample households. Accordingly, 138 sample households, 6 large traders, 12 small traders, 8 collectors 5 butchers, 7 hotels/restaurants and 11 consumers were interviewed through a semi-structured questionnaire and from key informant interview. The data were collected from both primary and secondary sources. Primary data were collected from randomly selected sample households through semi-structured questionnaire and checklists were used for key informants’ interviews. Secondary data were also collected from office of agriculture and rural development of the district. The performance of small ruminant market was analyzed using marketing margins. Small ruminant farmers’s share of the consumer’s price was highest along producers – consumers, producers – farmers (for breeding purpose) and producers – small traders – consumers in both goat and sheep market channels. Similarly, Producer’s share of the consumer’s price was lowest along producers – hotels/restaurants – consumers and producers – collectors – large traders –hotels/restaurants – consumers in both goat and sheep market channel. As the market channel becomes longer and involves more transactions between different actors, the producers’ share of the final price decreases. Therefore, effort should be made to establish producers’ cooperative, help farmers on giving training in appropriate technologies, marketing systems, working capital and information.
In Situ Generation of Organic Peroxide to Create a Nanotubular Uranyl Peroxide Phosphate
Authors: Kravchuk Dmytro V. , Forbes Tori Z. 
Published: October 2019

AbstractCurrent synthetic pathways for uranyl peroxide materials introduce high initial concentrations of aqueous H2O2 that decline over time. Alternatively, in situ generation of organic peroxide would maintain constant concentrations of peroxide over prolonged periods of time and open new pathways to novel uranyl peroxide compounds. Herein, we demonstrate this concept through the synthesis of a nanotube‐like uranyl peroxide phosphate (NUPP), Na12[(UO2)(μ‐O2)(HPO4)]6(H2O)40, making use of the inhibited autoxidation of benzaldehyde in benzyl alcohol solutions in the presence of phosphonate ligands. The unique feature of NUPP is the bent dihedral angle U‐(μ‐O2)‐U (123.9°±0.4° to 124.6°±0.5°), which allows hexameric uranyl peroxide macrocycles to adopt the nanotubular topology and prevents the formation of nanocapsules. Raman spectroscopy of the solution phase confirms our mechanistic understanding of the reaction pathway and confirms that consistent levels of peroxide are generated in situ over an extended period of time.
Modeling Alcohol Dehydrogenase Catalysis in Deep Eutectic Solvent/Water Mixtures
Authors: Huang Lei , Bittner Jan Philipp , Domínguez de María Pablo , Jakobtorweihen Sven , Kara Selin 
Published: October 2019

AbstractThe use of oxidoreductases (EC1) in non‐conventional reaction media has been increasingly explored. In particular, deep eutectic solvents (DESs) have emerged as a novel class of solvents. Herein, an in‐depth study of bioreduction with an alcohol dehydrogenase (ADH) in the DES glyceline is presented. The activity and stability of ADH in mixtures of glyceline/water with varying water contents were measured. Furthermore, the thermodynamic water activity and viscosity of mixtures of glyceline/water have been determined. For a better understanding of the observations, molecular dynamics simulations were performed to quantify the molecular flexibility, hydration layer, and intraprotein hydrogen bonds of ADH. The behavior of the enzyme in DESs follows the classic dependence of water activity (aW) in non‐conventional media. At low aW values (<0.2), ADH does not show any activity; at higher aW values, the activity was still lower than that in pure water due to the high viscosities of the DES. These findings could be further explained by increased enzyme flexibility with increasing water content.
Review on Recent Progress in the Development of Tungsten Oxide Based Electrodes for Electrochemical Energy Storage
Authors: Shinde Pragati A. , Jun Seong Chan 
Published: October 2019

AbstractCurrent progress in the advancement of energy‐storage devices is the most important factor that will allow the scientific community to develop resources to meet the global energy demands of the 21st century. Nanostructured materials can be used as effective electrodes for energy‐storage devices because they offer various promising features, including high surface‐to‐volume ratios, exceptional charge‐transport features, and good physicochemical properties. Until now, the successful research frontrunners have focused on the preparation of positive electrode materials for energy‐storage applications; nevertheless, the electrochemical performance of negative electrodes is less frequently reported. This review mainly focuses on the current progress in the development of tungsten oxide‐based electrodes for energy‐storage applications, primarily supercapacitors (SCs) and batteries. Tungsten is found in various stoichiometric and nonstoichiometric oxides. Among the different tungsten oxide materials, tungsten trioxide (WO3) has been intensively investigated as an electrode material for different applications because of its excellent charge‐transport features, unique physicochemical properties, and good resistance to corrosion. Various WO3 composites, such as WO3/carbon, WO3/polymers, WO3/metal oxides, and tungsten‐based binary metal oxides, have been used for application in SCs and batteries. However, pristine WO3 suffers from a relatively low specific surface area and low energy density. Therefore, it is crucial to thoroughly summarize recent progress in utilizing WO3‐based materials from various perspectives to enhance their performance. Herein, the potential‐ and pH‐dependent behavior of tungsten in aqueous media is discussed. Recent progress in the advancement of nanostructured WO3 and tungsten oxide‐based composites, along with related charge‐storage mechanisms and their electrochemical performances in SCs and batteries, is systematically summarized. Finally, remarks are made on future research challenges and the prospect of using tungsten oxide‐based materials to further upgrade energy‐storage devices.
Integrating population genetics to define conservation units from the core to the edge of Rhinolophus ferrumequinum western range
Authors: Tournayre Orianne , Pons Jean‐Baptiste , Leuchtmann Maxime , Leblois Raphael , Piry Sylvain , Filippi‐Codaccioni Ondine , Loiseau Anne , Duhayer Jeanne , Garin Inazio , Mathews Fiona , Puechmaille Sébastien , Charbonnel Nathalie , Pontier Dominique 
Published: October 2019

AbstractThe greater horseshoe bat (Rhinolophus ferrumequinum) is among the most widespread bat species in Europe but it has experienced severe declines, especially in Northern Europe. This species is listed Near Threatened in the European IUCN Red List of Threatened Animals, and it is considered to be highly sensitive to human activities and particularly to habitat fragmentation. Therefore, understanding the population boundaries and demographic history of populations of this species is of primary importance to assess relevant conservation strategies. In this study, we used 17 microsatellite markers to assess the genetic diversity, the genetic structure, and the demographic history of R. ferrumequinum colonies in the western part of its distribution. We identified one large population showing high levels of genetic diversity and large population size. Lower estimates were found in England and northern France. Analyses of clustering and isolation by distance suggested that the Channel and the Mediterranean seas could impede R. ferrumequinum gene flow. These results provide important information to improve the delineation of R. ferrumequinum management units. We suggest that a large management unit corresponding to the population ranging from Spanish Basque Country to northern France must be considered. Particular attention should be given to mating territories as they seem to play a key role in maintaining high levels of genetic mixing between colonies. Smaller management units corresponding to English and northern France colonies must also be implemented. These insular or peripheral colonies could be at higher risk of extinction in the near future.
Responses of soil moisture to regional climate change over the Three Rivers Source Region on the Tibetan Plateau
Authors: Deng Mingshan , Meng Xianhong , Li Zhaoguo , Lyv Yaqiong , Lei Huajin , Zhao Lin , Zhao Shengnan , Ge Jun , Jing Hui 
Published: October 2019

AbstractIn this study, soil moisture data from two reanalysis datasets (ERA‐Interim, ERA5), a satellite soil moisture product from the European Space Agency (ESA) and three assimilation datasets from the Global Land Data Assimilation System (GLDAS), that is, GLADS‐NOAH025, GLADS‐NOAH10, and GLDAS‐CLM were compared with the observed soil moisture data in Maqu and Maduo stations over the Three Rivers Source Region (TRSR) on the Tibetan Plateau in China. Comparative statistical parameters between the soil moisture observations and the products, including the root mean square error, correlation coefficient (R), and standard deviation ratio were calculated to evaluate the products in different seasons. It was found that most products overestimated the soil moisture values, among which GLADS‐NOAH025 had relatively good consistency with the observations in the surface soil layer (0–10 cm), whereas GLDAS‐CLM agreed well with the observations in the 10–40 cm layer. Both ERA5 and ERA‐interim soil moisture overestimated soil moisture at all levels. The responses of soil moisture to regional climate change over the TRSR were also investigated by using the GLDAS‐NOAH025 soil moisture product, the ESA soil moisture product, and the observations during the nonfreezing period. The results show that the trends of soil moisture are consistent with the precipitation changes in the main central part of the TRSR. A significant positive correlation was found between soil moisture and precipitation in most regions of the TRSR, which indicates that soil moisture increases with the increase of precipitation, except in the northwest TRSR, where the variation of soil moisture was possibly influenced by both the precipitation changes and the increase of evapotranspiration.
Improvement of the refrigerated preservation technology by hyperbaric storage for raw fresh meat
Authors: Santos Mauro D , Castro Rodolfo , Delgadillo Ivonne , Saraiva Jorge A 
Published: October 2019

AbstractBACKGROUNDThis work aimed to compare raw fresh meat (minced bovine and pork in pieces) preserved by hyperbaric storage (HS) at room‐like temperature (75 MPa/25 °C) and HS at cold temperatures (60 MPa/10 °C) for up to 60 days, being both compared to refrigeration (RF, 4 °C).RESULTSHS conditions showed microbial load reductions over 60 days of storage, leading to a possible shelf‐life extension when compared to samples at RF. Moreover, between both HS conditions similar results were found at the 60th day, reaching in some cases values < 1.00 log CFU g−1. Overall, pH presented an increase with storage for both HS conditions (e.g. over 30 days, from 5.51 ± 0.02 to 5.70 ± 0.01 and 5.85 ± 0.03, for 60 MPa/10 °C and 75 MPa/25 °C, respectively, on pork meat in pieces, PP) contrary to RF where pH values decreased (from 5.51 ± 0.02 to 5.33 ± 0.03). Regarding moisture content and drip loss, lower and higher values were found, respectively at 75 MPa/25 °C, mainly in bovine minced meat. Overall, colour ΔE* did not present considerable differences for both samples under all storage conditions. Lipid oxidation presented an increase tendency over time, with both HS conditions showing the higher values (1.795 ± 0.217 and 2.169 ± 0.117 for 60 MPa/10 °C and 75 MPa/25 °C, respectively, compared to 0.895 ± 0.084 μg MDA g−1 in PP samples at the 30th day).CONCLUSIONAlthough several advantages were found further studies should be carried out in order to optimize the HS conditions for raw fresh meat and assess the impact of this preservation methodology on other meat quality parameters as for instance sensorial aspects. © 2019 Society of Chemical Industry
Identification of ligands from natural products as inhibitors of glutathione S‐transferases using enzyme immobilized mesoporous magnetic beads with high‐performance liquid chromatography plus quadrupole time‐of‐flight mass spectrometry and molecular docking
Authors: Yi Renxing , Fu Renjie , Li Dapeng , Qi Jin , Liu Haichun 
Published: October 2019

AbstractMultidrug resistance is recognized as one of the main reasons leading to the failure of chemotherapy. Studies have shown that glutathione S‐transferase inhibitors could be regarded as multidrug resistance reversal agents. Herein, a method of applying enzyme immobilization, molecular docking, and high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry was employed to screen glutathione S‐transferase inhibitors from natural products. Magnetic mesoporous silica microspheres were synthesized and modified with a poly(dopamine) layer, which has a large quantity of amino, enabling further non‐covalent binding with glutathione S‐transferase. Moreover, the immobilization conditions, namely, potential of hydrogen, catalase concentration, reaction temperature and reaction time, were optimized. In total, six potential compounds were isolated and identified from Perilla frutescens (L.) Britt leaves and green tea and molecular docking was applied to identify the binding site. Rosmarinic acid, (−)‐epigallocatechin‐3‐O‐gallate and (−)‐epicatechin‐3‐gallate showed higher binding affinity than the compounds, and their half maximal inhibitory concentration values were further determined. The results suggested that this proposed method was effective and convenient for identifying glutathione S‐transferase inhibitors from natural products.