bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of miR‐579 on the PI3K/AKT pathway in human glioblastoma PTEN mutant cell lines
Authors: Kalhori Mohammad Reza , Irani Shiva , Soleimani Masoud , Arefian Ehsan , Kouhkan Fatemeh 
Published: June 2019

AbstractGlioblastoma multiform (GBM) is a type of aggressive brain cancer with limited success in standard treatment. MicroRNAs are one of the most beneficial tools for diagnosis, prognosis, and treatment of cancer. This study aimed to investigate the effect of miR‐579 on cellular behaviors and expression of PI3K/AKT signaling pathway in GBM cell lines. In the present study, miR‐579 was overexpressed in U251 and A‐172 cell lines by using lentil vector, and its effect on cellular behavior such as proliferation and migration was investigated by the cell cycle, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), Annexin V, colony formation, Transwell and wound healing assays. MiR‐579 predicted target genes (AKT1, Rheb, PDK1, and a few others) were also evaluated by real‐time polymerase chain reaction or luciferase assay and Western blot analysis. Our results represented that overexpression of miR‐579 could inhibit proliferation, migration, cell cycle and also promoted the apoptosis of GBM cell lines. The luciferase reporter assay showed miR‐579 directly targets the 3 UTR of mTOR, Rheb, and PDK1 and repressed their expressions. Furthermore, the Western blot analysis showed that miR‐579 could downregulate the AKT1 and Rheb protein expression. Overall, our findings propose that miR‐579 functions as a novel tumor suppressor gene in GBM by regulating the PI3K/AKT signaling pathway and may serve as a therapeutic target for clinical therapy of glioblastoma multiform.
Saffron (Crocus sativus) in the treatment of gastrointestinal cancers: Current findings and potential mechanisms of action
Authors: Naeimi Maryam , Shafiee Mojtaba , Kermanshahi Farnoush , Khorasanchi Zahra , Khazaei Majid , Ryzhikov Mikhail , Avan Amir , Gorji Narjes , Hassanian Seyed M. 
Published: June 2019

AbstractGastrointestinal (GI) cancers are major causes of cancer‐related mortality worldwide and include malignancies of the GI tract such as the stomach, liver, pancreas, small intestine, colon, and rectum. Promising and selective anticancer effects of pharmacologically active components of saffron (Crocus sativus L.) have been shown in preclinical in vitro and in vivo studies. Saffron and its active components including crocin, crocetin, and safranal exert their anticancer effects through different mechanisms, including induction of apoptosis, influence on the cell cycle, and regulation of host immune response and anti‐inflammatory activities. This review summarizes the recent literature on the chemopreventive properties of saffron in GI cancers to have a better understanding of the potential underlying mechanisms and hence the appropriate management of these malignancies.
lncRNA HNF1A‐AS1 modulates non–small cell lung cancer progression by targeting miR‐149‐5p/Cdk6
Authors: Liu Lu , Chen Yanzhi , Li Qiaoqing , Duan Peizeng 
Published: June 2019

AbstractGrowing evidence have shown the important regulation of lncRNAs (long noncoding RNAs) in non–small cell lung cancer (NSCLC). lncRNA hepatocyte nuclear factor 1 homeobox A (HNF1A)‐antisense RNA 1 (AS1), an “oncogene”, was reported to regulate human tumors progression. However, the molecular mechanism of HNF1A‐AS1 involved in the development of NSCLC is still under investigation. In the current study, we found that HNF1A‐AS1 was relatively upregulated in both NSCLC patient tissues and cell lines. Functional studies established that overexpression of HNF1A‐AS1 promoted cell proliferation, cell cycle, invasion, and migration of NSCLC cells in vitro. The promotion abilities of HNF1A‐AS1 on NSCLC cell progression were suppressed via knockdown of HNF1A‐AS1. miR‐149‐5p was then proved to be a novel target of HNF1A‐AS1, whose expression was negatively correlated with HNF1A‐AS1 in NSCLC patient tissues and cell lines. HNF1A‐AS1 increased the expression of cyclin‐dependent kinase 6 (Cdk6) via sponging with miR‐149‐5p. Gain‐ and loss‐of‐functional studies indicated that HNF1A‐AS1 promoted NSCLC progression partially through inhibition of miR‐363‐3p and induction of Cdk6. Subcutaneous xenotransplanted tumor model confirmed that interference of HNF1A‐AS1 suppressed the tumorigenic ability of NSCLC via upregulation of miR‐149‐5p and downregulation of Cdk6 in vivo. In conclusion, our findings clarified the biologic significance of the HNF1A‐AS1/miR‐149‐5p/Cdk6 axis in NSCLC progression and provided novel evidence that HNF1A‐AS1 may be a new potential therapeutic target for the treatment of NSCLC.
The relentless pursuit of perfectionism: A review of perfectionism in the workplace and an agenda for future research
Authors: Ocampo Anna Carmella G. , Wang Lu , Kiazad Kohyar , Restubog Simon Lloyd D. , Ashkanasy Neal M. 
Published: June 2019

SummaryThe pursuit of perfectionism resonates with many individuals across workplaces resulting in a recent flurry of research on the topic. Although extant research has examined the costs and benefits of perfectionism at work, these efforts are scattered across multiple disciplines and utilize varying conceptualizations. As a result, we lack a coherent understanding of how perfectionism influences work behavior. To address this issue, we integrate the nascent but fragmented perfectionism at work literature, including both empirical findings and theoretical perspectives. We introduce and discuss a future research agenda that addresses not only the need to broaden understanding of perfectionism's antecedents, processes, and boundary conditions but also its multilevel applications and methodological limitations. Our review will enable organizational scholars to develop a deeper understanding of how perfectionism renders its influence in the workplace.
Architectural and anatomical responses of maize roots to agronomic practices in a semi‐arid environment
Authors: Zhan Ai , Liu Jianliang , Yue Shanchao , Chen Xinping , Li Shiqing , Bucksch Alexander 
Published: June 2019

AbstractRoot architecture and anatomy are important determinants of nitrogen (N) and water acquisition, but they are also environmentally plastic to adapt to N and water availability. Therefore, understanding the relationship between root traits and environmental factors is essential for improving N and water acquisition. A field experiment was conducted in the semi‐arid region of the Loess Plateau in northwestern China to quantify the architectural and anatomical root traits of maize (Zea mays L.) in response to plastic film mulching and N fertilization. We compared four treatments: non‐mulching with and without N supply as well as plastic film mulching with and without N supply. Variation existed for all root architecture and anatomy traits within maize root crowns. Crown and brace root angles to the soil line decreased in response to film mulching and N fertilization. Crown roots under plastic film mulching showed a significantly decreased distance to branching, reduced lateral root length, and overall increased root diameter. Similarly, N application significantly decreased the distance to branching, yet induced more compact and denser crown roots, and increased the root diameter. Brace roots exhibited an increased distance to branching, greater lateral root length and density, as well as a larger root diameter in response to plastic film mulching and N fertilization. Additionally, the accumulated number of nodal roots increased greatly under plastic film mulching and N treatments. At the anatomical level, N application reduced the proportion of the root cortical aerenchyma area. In contrast, aerenchyma area, cortex cell size, and late metaxylem vessel diameter were increased as a result of plastic film mulching. These results demonstrate root architectural and anatomical traits respond to mulching practices and N fertilization.
A column perfusion test to assess the kinetics of nutrient release by soluble, sparingly soluble and coated granular fertilizers
Authors: Baird Roslyn , da Silva Rodrigo C. , Degryse Fien , McLaughlin Michael J. 
Published: June 2019

AbstractIn recent years, innovations in fertilizer research have principally been in the design of products to supply more timely plant nutrients and mitigate detrimental losses to the environment. In the development of these fertilizers, a laboratory method to comparatively screen a large number of formulations for the rate of nutrient release is a critical first step in formulation optimization to significantly reduce time spent on pot and field trials, reducing costs considerably. Currently, given the absence of a standard laboratory method for all fertilizers, adaptions of a method for coated fertilizers from theInternational Organization for Standardization(2017) ISO 21263 and other column tests are being used which are often complex and/or require manual sampling which can be laborious, time consuming and costly. The method presented here evaluates the dynamic release in real time by continuously perfusing samples in columns utilizing an automated sample accumulation system for short‐term and long‐term release studies and eliminates the need for soil/sand packing of columns. Mass balance is accounted for by analysing the residue after dissolution and the amount released is expressed as a % of the total nutrient content in the product. This method is reliable, uncomplicated and cost‐effective and has been used to test uncoated soluble fertilizers, nanofertilizers, fertilizers with newly developed coating technologies and other slowly soluble fertilizers, to assess the effect of variables like coating chemistry and thickness, fertilizer composition or fertilizer particle size on release rate.
Aqueous Foam Stabilized by Hydrophobic SiO2 Nanoparticles using Mixed Anionic Surfactant Systems under High‐Salinity Brine Condition
Authors: Rattanaudom Pattamas , Shiau Bor‐Jier , Suriyapraphadilok Uthaiporn , Charoensaeng Ampira 
Published: June 2019

AbstractA primary concern of surfactant‐assisted foams in enhanced oil recovery (EOR) is the stability of the foams. In recent studies, foam stability has been successfully improved by the use of nanoparticles (NP). The adhesion energy of the NP is larger than the adsorbed surfactant molecules at the air–water interface, leading to a steric barrier to mitigate foam‐film ruptures and liquid‐foam coalescence. In this study, the partially hydrophobic SiO2 nanoparticles (SiO2‐NP) were introduced to anionic mixed‐surfactant systems to investigate their potential for improving the foamability and stability. An appropriate ratio of internal olefin sulfonate (C15‐18 IOS) and sodium polyethylene glycol monohexadecyl ether sulfate (C32H66Na2O5S) was selected to avoid the formation of undesirable effects such as precipitation and phase separation under high‐salt conditions. The effects of the NP‐stabilized foams were investigated through a static foam column experiment. The surface tension, zeta potential, bubble size, and bubble size distribution were observed. The stability of the static foam in a column test was evaluated by co‐injecting the NP‐surfactant mixture with air gas. The results indicate that the foam stability depends on the dispersion of NP in the bulk phase and at the water–air interface. A correlation was observed in the NP‐stabilized foam that stability increased with increasing negative zeta potential values (−54.2 mv). This result also corresponds to the smallest bubble size (214 μm in diameter) and uniform size distribution pattern. The findings from this study provide insights into the viability of creating NP‐surfactant interactions in surfactant‐stabilized foams for oil field applications.
Intracorporeal circular‐stapled anastomosis after totally laparoscopic gastrectomy: A novel, simplest u‐shaped parallel purse‐string suture technique
Authors: Du Jianjun , Xue Hongyuan , Zhao Lizhi , Hua Jin , Hu Jian , Zhang Ziqiang 
Published: June 2019

AbstractBackgroundIntracorporeal anastomoses in totally laparoscopic gastrectomy (TLG) remain technical challenges to many surgeons, although the intracorporeal jejunojejunal or gastrojejunal anastomosis is an essential procedure during TLG. Standardized, reproducible and simple circular‐stapled anastomosis in open gastrectomy is limited in TLG due to the difficulties of intracorporeal purse‐string suture or anvil placement. An optimal procedure for intracorporeal anastomosis in TLG remains to be established.MethodsBetween February 2018 and January 2019, 31 consecutive patients with gastric cancer underwent totally laparoscopic total gastrectomy (TLTG) or totally laparoscopic distal gastrectomy (TLDG) using the novel u‐shaped parallel purse‐string suture technique along the jejunum for anvil placement. The intracorporeal circular‐stapled jejunojejunostomy of Roux‐en‐Y reconstruction in TLTG and gastrojejunostomy of Billroth II in TLDG were, respectively, performed.ResultsThe total mean  ± SD operative time was 214.7 ± 48.6 minutes. The time required for purse‐string suture and anvil placement was 2.3 ± 0.5 and 4.4 ± 1.1 minutes, respectively. There were no instances of postoperative jejunojejunal or gastrojejunal anastomosis‐related complications observed during the median follow‐up period of 5.5 months.ConclusionsThe novel procedure conceptionally and technically changes the conventional circular‐shaped purse‐string suture into a much simpler way, u‐shaped parallel purse‐string suture. This could be the simplest published intracorporeal pure‐string suture technique
Molecularly imprinted polymer film grafted from porous silica for efficient enrichment of steroid hormones in water samples
Authors: Czarny Karolina , Szczukocki Dominik , Krawczyk Barbara , Juszczak Renata , Skrzypek Sławomira , Gadzała‐Kopciuch Renata 
Published: June 2019

AbstractSteroid hormones as endocrine disrupting compounds can interfere with the functioning of hormonal systems of organisms and thus affect the health and reproduction of humans and wildlife. Unfortunately, these types of harmful endocrine disrupting compounds have been found in a variety of environmental samples at very low concentrations. Therefore, a simple, fast, and efficient method for enrichment of water samples is needed. A molecularly imprinted solid‐phase extraction combined with high performance liquid chromatography coupled with diode array detection was developed for the determination of six steroid hormones, such as estrone, 17‐β‐estradiol, estriol, 17‐α‐ethinylestradiol, progesterone, and testosterone in water samples. The recoveries obtained in the proposed method were in the range of 78.7–101.3%. Matrix effect below 20% suggests that the quantitative and qualitative results of the analysis were not significantly affected by the matrix. The results show that molecularly imprinted polymers based on spherical silica gel had the potential to be a highly innovative and selective sorbent. The proposed method was proved to be applicable for molecularly imprinted solid‐phase extraction in selective and reliable extraction and enrichment of steroid hormones in environmental water samples.
Inter‐ and Intra‐rater Agreement of Dermatologic Ultrasound for the Diagnosis of Lobular and Septal Panniculitis
Authors: Romaní Jorge , Giavedoni Priscila , Roé Esther , Vidal David , Luelmo Jesús , Wortsman Ximena 
Published: June 2019

ObjectivesDermatologic ultrasound (US) may aid in the diagnosis and classification of panniculitis. The purpose of this study was to assess the capability of dermatologic US for subtyping mainly septal/lobular panniculitis.MethodsA multicentric and prospective study of the inter‐ and intra‐rater agreement of dermatologic US for subtyping panniculitis was conducted among 4 clinicians with experience in dermatologic US and a radiologist specialized in dermatologic US. Clinicians recruited patients and performed dermatologic US examinations of the most substantial lesion and punch biopsies. A histologic study was considered the reference standard. Then the images were blindly evaluated by all researchers. For intra‐ and inter‐rater agreement, Cohen and Fleiss κ values were calculated.ResultsSixty‐four patients were included. The Cohen intra‐rater κ was 0.74. Sensitivity and specificity for lobular panniculitis were 85.19 and 88.57, respectively. The Fleiss inter‐rater κ was 0.47. Limitations of the study included the small number of patients and differences in evaluators and their dermatologic US equipment.ConclusionsThis study supports the use of US for diagnosing panniculitis. For subtyping panniculitis, the intra‐rater correlation was good. Improvement of inter‐rater agreement may depend on access to clinical information, dynamic images, a better definition of criteria, homogeneous configurations of the devices, and the expertise of dermatologic US operators.
Workforce analysis of practicing rhinologists in the united states
Authors: Heineman Thomas E. , Ramakrishnan Vijay , Hwang Peter H. , Suh Jeffrey D. 
Published: June 2019

ObjectivesThe objective of this study is to analyze the current rhinology workforce in the United States in terms of demographics, fellowship training, and regional distribution.MethodsThe American Rhinologic Society (ARS) member database was queried for workplace zip code, age, gender, and fellowship training of practicing U.S. rhinologists through 2017. The San Francisco Match data were examined for rhinology fellowships from 2006 through 2017. Each rhinologist was assigned to a specific hospital referral region (HRR) determined by the Dartmouth Healthcare Atlas to assess geographic distribution and market density.ResultsThere were 296 ARS members self‐identifying as rhinologists. Of those, 69.2% were fellowship‐trained (FTR). The median age of FTRs and non‐FTRs were 41 and 54 years, respectively (P < 0.001). Eighty percent of FTRs were male compared to 85.7% non‐FTRs. Given the recent growth of rhinology fellowships, the number of FTRs is currently expanding at approximately 16% per year. HRRs with the most rhinologists were Los Angeles, Boston, and Manhattan, all with 14. Approximately 100 million people live in HRRs without a practicing rhinologist. A wide variation in rhinologist density was observed, ranging from 133,047 to 3,636,809 people per rhinologist, with a median of 747,864.ConclusionThere may be a need for rhinologists in select parts of the country. Whereas the ARS database is likely an underestimation of the growing workforce, under current training rates we could pass the ideal number of U.S. rhinologists within 5 years.Level of EvidenceNALaryngoscope, 130:1116–1121, 2020
Sleep apnea and the impact on cardiovascular risk in patients with Marfan syndrome
Authors: Muiño‐Mosquera Laura , Bauters Fré , Dhondt Karlien , De Wilde Hans , Jordaens Luc , De Groote Katya , De Wolf Daniel , Hertegonne Katrien , De Backer Julie 
Published: June 2019

AbstractBackgroundMarfan syndrome (MFS) is an inherited connective tissue disorder characterized by ectopia lentis, aortic root dilation and dissection and specific skeletal features. Obstructive sleep apnea (OSA) in MFS has been described earlier but the prevalence and its relation with the cardiovascular risk is still controversial. This study aimed to further investigate these aspects.MethodsIn this prospective longitudinal study, we performed an attended polysomnography in 40 MFS patients (60% women, 37 ± 12.8 years) and evaluated several cardiovascular parameters through echocardiography, resting electrocardiogram, 24 hr‐Holter monitoring and serum NT‐ProBNP measurements.ResultsWe found that OSA was present in 42.5% of the patients and that higher body mass index was the most important factor associated with the presence of OSA. We observed that overweight was present in 27.5% of the patients in the whole cohort and in 55.6% if >40 years. Furthermore, when evaluating the impact of OSA on the cardiovascular system, we observed that patients with OSA tended to have higher systolic blood pressure, larger distal aortic diameters and a higher prevalence of ventricular arrhythmia. These differences were, however, not significant after adjusting for confounders.ConclusionsOur study shows a high prevalence of OSA and a high prevalence of overweight in MFS patients. We found some trends between OSA and cardiovascular features but we could not establish a solid association. Our study, however might be underpowered, and a multicenter collaborative study could be very useful to answer some important open questions.
Effects of chain extender and uniaxial stretching on the properties of PLA/PPC/mica composites
Authors: Xia Menglu , Shi Kunxiang , Zhou Mingzhu , Shen Yucai , Wang Tingwei 
Published: June 2019

Poly(lactic acid) (PLA)/poly(propylene carbonate) (PPC)/mica composites with different amount of chain extender (CE) were melt compounded and then processed via two routes (compression molding and uniaxial stretching) into sheets and films. The tensile, thermal, and oxygen barrier properties of all the samples were investigated. Tensile test showed that the tensile strength and elongation at break of all films were much higher than that of all sheets, especially for PLA/PPC/mica with 0.9‐wt% CE composite (CM3(CE)0.9) film. The crystallinity of all films increased significantly after uniaxial stretching of sheet samples. The Fourier transform infrared spectroscopy (FTIR) results proved the chemical reactions occurred between PLA/PPC and CE. Scanning electron microscope (SEM) analysis revealed that compatibility and interfacial adhesion of all samples were improved after adding mica and CE, and they were further enhanced after uniaxial stretching. The addition of CE was not favorable to improve the oxygen barrier performance of PLA/PPC/mica sheet samples. However, the oxygen barrier performance of film samples was significantly improved after uniaxial stretching. In particular, the CM3(CE)0.9 film had the lowest oxygen permeability coefficient (1.4 × 10−15 cm3·cm/(cm2·s·Pa)), and this was the best oxygen barrier properties reported in the literature for PLA‐based composites, which was comparable with PA film. This study demonstrated the high efficiency of uniaxial stretching on improvement of properties of composites, which would promote the application of biodegradable polymers in oxygen sensitive food packaging.
Nonclassical B‐Hb⋯π interaction in diborane⋯localized‐π sandwiches: A DFT‐D3 study
Authors: Saha Bapan , Sharma Himakshi , Bhattacharyya Pradip Kr. 
Published: June 2019

AbstractThe study presents a quantitative estimation of B‐Hb⋯π interaction in diborane‐localized‐π half sandwiches and sandwiches. DFT‐D3 method is used for geometry optimization and estimating the stability of the complexes (in terms of stabilization energies). The diborane molecule is the source of bridging hydrogen and O2, C2H4, and C2H2 molecules are considered as the localized π‐systems. MP2 and CCSD(T) calculations are also performed to measure the stability of the chosen complexes. Results indicate that the complexes are feebly stable in gas phase with stabilization energies <5.0 kcal mol−1. Compared to DFT‐D3 functionals, MP2 calculations are found to be more suitable in predicting the stability of the complexes. Dispersive interaction is the primary mode of interaction in stabilizing the complexes. Presence of substituents either on diborane or on the considered π‐systems play key role in stabilizing the complexes. Thermochemical analysis demonstrates the exothermic nature of complexation. 1H and 13B NMR analysis are also performed.