bluuebunny/crossref_metadata_2025_split huggingface dataset:
Findings and Management of the Rare Caput Flexum Deformity of the Hip
Authors: Leunig M. , Fickert S. , Ganz R. 
Published: August 2019

Case:
A 10-year-old girl presented after possible occult hip trauma, with shortening of the leg being the initial clinical symptom, followed by motion-dependent pain. She had limited external rotation in extension with anterior apprehension. Radiographically, the deformity was an anterior tilt of the epiphysis with coxa vara. Surgery included surgical dislocation using a retinacular flap for the anterior open wedge femoral neck osteotomy for extension and posterior translation, with an excellent 4.5-year clinical outcome.

Conclusions:
Caput flexum is a rare deformity with localized premature closure of the anterior growth plate of the hip. To avoid secondary impingement, an osteotomy was successfully placed close to the deformity.
Intramedullary Insertion of the Patellar Tendon, a Rare Anatomical Variant, Its Sequelae, and Surgical Management
Authors: Collett David J. , Sharma Rahul , Goyal Prateek 
Published: August 2019

Case:
We report a rare cause of patella baja in a 25-year-old man presenting with right knee pain. He was found to have patella baja and a genu recurvatum deformity. Imaging revealed an extremely unusual variant of the patellar tendon—identified to have an intramedullary tibial insertion. A patellar tendon reconstructive procedure was performed.

Conclusions:
An intramedullary insertion of the patellar tendon is a fascinating and rare cause of patella baja with subsequent altered biomechanics of the extensor mechanism. We believe this is the first case report to describe its surgical presentation and an operative approach to its management.
Characterization of the Intestinal Microbiota and Metabolites of Broiler Breeders with Different Egg Laying Rate
Authors: Yang Zengqiao , Wang Jianping , Celi Pietro , Zhang Keying , Ding Xuemei , Bai Shiping , Zeng Qiufeng , Mao Xiangbing , Feng Bing 
Published: August 2019

Abstract
BackgroundThe aim of the present study was to determine differences in the intestinal microbiota and metabolites of broiler breeders with different egg laying rate; and its possible relationships between intestinal microbiota and intestinal metabolites were also explored.MethodsA total of 200 AA+ parent broiler breeders (41-week-old) were separated into two groups according to their different egg laying rate: average reproductive group (AR: 77.26%±0.88%) and high reproductive group (HR: 86.67%±0.75%), with 10 replicates and 10 birds each 42-days study.ResultsFeed conversion ratio (FCR), ovary cell apoptosis rate (ApoCR) and abdominal fat pad weight percentage were lower (p < 0.05), while the hatchability of qualified egg rate (HQR) was higher (p < 0.05) in HR group than that in AR group. At phylum level, Firmicutes and Proteobacteria were the dominant bacteria in small intestines, while Bacteroidetes was dominant bacteria in cecum. At genus level, Lactobacillus was dominant in small intestines while Bacteroides were dominant in cecum. In HR Phascolarctobacterium were lower (p < 0.05) in ileum. A positive correlation was observed between Actinobacteria in the small intestines and ApoCR (r = 0.34 & p < 0.05). A positive correlation was observed between FCR and Spirochaetes in cecum (r = 0.54 & p < 0.05). The metabolomics analysis on ileal digesta content revealed a higher concentration of Octadecanoic acid, 9,12-Octadecadienoic acid, 11-trans-Octadecenoic acid, d-Ribose and Inositol in HR broiler breeders. Moreover, inositol was enriched in "Phosphatidylinositol signaling system", "Inositol phosphate metabolism", "Ascorbate and aldarate metabolism", "Galactose metabolism", "ABC transporters", and Octadecanoic acid was enriched in "Fatty acid biosynthesis", "Biosynthesis of unsaturated fatty acids".ConclusionsOur results suggest that glycerol, inositol modulate energy partitioning in fat metabolism to influence reproductive performances. Microbiota, such as Actinobacteria (phylum), Spirochaetes (phylum), and Phascolarctobacterium (genus) may have negative relationship with broiler breeders’ reproductive performances.
DNA methylation in blood - potential to provide new insights in immune cell biology
Authors: Macartney-Coxson Donia , Cameron Alanna , Clapham Jane , Benton Miles C 
Published: August 2019

Abstract
BackgroundEpigenetics plays a fundamental role in cellular development and differentiation; epigenetic mechanisms, such as DNA methylation, are involved in gene regulation and the exquisite nuance of expression changes seen in the journey from pluripotency to final differentiation. Thus, DNA methylation has the potential to reveal new insights in to immune cell biology.ResultsWe mined publicly available DNA methylation data with a machine-learning approach to identify differentially methylated loci between different white blood cell types. We then interrogated the DNA methylation and mRNA expression of candidate loci in CD4+, CD8+, CD14+, CD19+ and CD56+ fractions from 12 additional, independent healthy individuals (6 male, 6 female). ‘Classic’ immune cell markers such as CD8 and CD19 showed expected methylation/expression associations fitting with established dogma that hypermethylation is associated with the repression of gene expression. We also observed large differential methylation at loci which are not considered established immune cell markers, and some of these novel loci showed inverse correlations between methylation and mRNA expression (such as PARK2,DCP2).ConclusionsOur results highlight the value of mining publicly available data, the utility of DNA methylation as a discriminatory marker and the potential value of DNA methylation to provide additional insights into immune cell biology and developmental processes.
Risk Factors for Low Birth Weight among Neonates Delivered in Public Health Facilities in Adama town, Oromia Regional State, Ethiopia
Authors: Nebi Nigusse Obse , Chaka Tolossa Eticha , Abebe Tilaye Workineh , M Ephrem Mannekulih 
Published: August 2019

Abstract
Back ground: Low birth weight is the major predictor of prenatal mortality and morbidity world wide. It has been defined by the World Health Organization as weight at birth of less than 2,500 grams irrespective of their age. Rate of low birth weight is still high in developing countries like Ethiopia particularly Oromia regional state where adequate primary health care services for maternal and child health are not universally available to all the populations. It is therefore imperative to identify risk factors for low birth weight in various communities in order to come up with feasible intervention strategies to minimize the problem.
Methods: Facility based case-control study design was conducted from June-1/2017 to April-30/2018 on 318 mothers with singleton and full term neonates (108 case to 210 control). Semi structured interviewer administered and pretested questionnaire was used by trained data collectors working in delivery ward. The data were entered and analyzed statistical software. Descriptive and bivariate analysis was done.
Result
The mean maternal age of all study participants was 26.7 years with [SD of 4.8] with mean age for mothers of cases was 25.5 years and for controls was 27.4 years. In bivariet analysis residency being rural (AOR= 1.95 with 95% CI (1.0-3.48), parity ≥2 (AOR= 3.45 (1.89-6.32), number of antenatal care attendance ˂4 visits (AOR= 0.40(0.218-0.73)), birth interval ˂24 moths (AOR= 2.68 (1.45-4.94), history of hypertension (AOR= 0.39(0.18-0.87) and maternal MUAC ˂21cm (AOR=0.38 (0.159-0.91) were found to be statistically significant.
Conclusions
Variables that were found to have a statically significant relationship with low birth weight were residency being rural, occupation, parity ≥2 & birth interval ≤24months, number of antenatal care attendance ˂4 visits, history of hypertension and maternal MUAC ˂21cm were found to be statistically significant.
Key phrases:- Low bith weight, maternal risk factor
Two SNPs rs1801133 and rs1801394 in folate pathway are associated with the risk of nonsyndromic cleft lip with or without cleft palate
Authors: Li Qiuyan , Xu Lidan , Jia Xueyuan , Zaib Tahir , Sun Wenjing , Fu Songbin 
Published: August 2019

Abstract
Background Prenatal intake of folic acid is important for prevention of nonsyndromic cleft lip with or without cleft palate (NSCL/P), genes participant in folate pathway are crucial for preventing birth defects. The present study aims to investigate the associations between four single nucleotide polymorphisms (SNPs) in folate pathway genes and the risk of NSCL/P.
Methods Prediction by bioinformatics was conducted to assess the function of genetic variation. The PubMed, Embase database and Google Scholar were searched by two researchers to identify all relevant studies. Stata 11.0 software was used to calculate the results. Subgroup analysis was conducted to assess the influence of the genetic background. Sensitivity analysis, regression analysis and publication analysis were conducted to improve the strength of the results.
Results Two genetic variations rs1801394 in MTRR gene and rs1801133 in MTHFR were predicted damaging. A total of 34 publications were included in the present analysis. The results showed that there were a significant association between rs1801133 and NSCL/P risk in two genetic models TT allele vs CC allele (OR=1.333 95%CI=1.062-1.674, P=0.013) and recessive model (OR=1.325 95%CI=1.075-1.634, P=0.008) and there were a significant association between rs1801394 and NSCL/P risk in Asian (GG allele vs AA allele, OR=0.520 95%CI=0.321-0.841, P=0.008). Meta-regression, sensitivity analysis, and publication bias analysis confirmed that the results of the present study were statistically robust.
Conclusions This present study suggests that rs1801133 of the MTHFR gene and rs1801394 of the MTRR gene are risk factors for NSCL/P. Additional, larger studies should be performed to confirm these findings.
Obesity is potentially associated with a novel mitochondrial tRNAGly 10029A&gt;G mutation in a Chinese family
Authors: Wang Jinling , Zhao Ningning , Mao Xiaoting , Meng Feilong , Huang Ke , Dong Guanping , Ji Yanchun , Fu JunFen 
Published: August 2019

Abstract
Background

Obesity is one of the most prominent public health challenges globally. Mutations in mitochondrial genes can similarly lead to the onset of childhood obesity remains unclear.

Methods

we conducted a clinical, genetic, and molecular profiling of a Han Chinese family with evident of matrilineally-inherited obesity. Obesity was evident in 2/6 matrilineal relatives in a single generation of this family (of 3 available generations). When the mitochondrial DNA of these individuals was sequences and a pedigree analysis was performed.

Results

We were able to identify a novel homoplasmic mutation of the mitochondrial tRNAGly gene (10029A>G) in these individuals. This mutation was linked to destabilization of a conserved base pair in the anticodon of this tRNA. This position (position 43) is known to be important for mediating effective codon recognition and tRNA stability. Consistent with the importance of this conserved site, we found that the predicted structure of a tRNAGly structure bearing a 10029A>G mutation was markedly altered in a molecular dynamics simulation relative to the wild-type isoform. All other mutations identified in the mtDNA of this individual were known variants associated with Asian haplogroup D4.

Conclusion

Our report provides novel evidence of a link between a tRNA mutation and an elevated risk of maternally-transmissible obesity risk, offering potentially novel insights into the underlying nature of obesity.
Characterizing a focused landscape of familial acute respiratory distress syndrome
Authors: Toby Inimary , Thomas Neal , Thorenoor Nithyananda , Spear Debbie , DiAngelo Susan , Floros Joanna 
Published: August 2019

Abstract
Background Acute respiratory distress syndrome (ARDS) affects approximately 190,600 patients per year in the United States, with mortality up to 45%. ARDS can occur as primary disease due to various factors (e.g. bacterial or viral pneumonia, gastric aspiration, lung contusion, toxic inhalation, and near drowning) or as secondary disease due to sepsis, pancreatitis, severe trauma, massive blood transfusion, and burn. We hypothesized that ARDS-affected individuals have patterns of variants in their physiological repertoire that can be tracked and utilized to complement clinical diagnosis and/or clinical monitoring. Methods The goals of this study were to: (1) characterize the landscape of variants within protein coding but we also studied UTR regions in ARDS using an Exome sequencing approach; (2), determine the variations in signaling pathways across ARDS; and (3) use computational approaches to explore the functional consequences of ARDS. Towards this we assessed an ARDS-affected individual in the context of unaffected individuals from the same family as well as unrelated ARDS cases, in order to elucidate underlying inheritance patterns of “private variants”. Private variants consist of variants shared by ARDS cases but not found in unaffected individuals. Results Whole exome sequencing yielded 3,516 variants represented by 2,354 genes. Of these, 128 variants were shared across all ARDS cases. Of these, there were 24 unique variants represented by 9 ARDS genes shared by the primary ARDS-case and unrelated individuals with ARDS. The overall genes identified and subsequent analysis, demonstrate that there are important biological pathways that distinguish ARDS cases from or from non-ARDS. These pathways include: cell-to-cell signaling interaction, cell growth and proliferation and cell morphology. The data also show a coordinated effort amongst biological processes such as liver hyperproliferation and cell death that underlie the pathogenesis of ARDS. Conclusions These in-silico discoveries demonstrate a role for private variants shared by ARDS cases to be leveraged as biomarkers for clinical diagnosis and/or monitoring of ARDS.
Pilot study of expanded newborn screening for 573 genes related to severe diseases in China: results from 1173 newborns
Authors: Luo Xiaomei , Sun Yu , Xu Feng , Guo Jun , Li Lin , Lin Zhiwei , Ye Jun , Gu Xuefan , Yu Yongguo 
Published: August 2019

Abstract
Background: Current newborn screening (NBS) in China is mainly aimed at detecting biochemical levels of metabolites in blood, which may generate false positive/negative results. Methods: We designed a next-generation sequencing (NGS) panel of 573 genes related to severe diseases and performed NBS for 1173 individuals, who had been screened for tandem mass spectrometry (MS/MS), phenylalanine (Phe), thyroid-stimulating hormone (TSH), 17-α-hydroxyprogesterone (17-OHP) and glucose-6-phosphate dehydrogenase (G6PD) as conducted in traditional biochemical NBS in September 2016. We compared their biochemical results with genetic variants and investigated the carrier frequencies of 77 genes related to disorders of MS/MS in these newborns. Results: Biochemical results showed that four newborns were positive for G6PD enzymatic assay (all males), the other biochemical values including MS/MS, Phe, TSH and 17-OHP were negative. Genetic analysis results showed that all the four newborns with positive G6PD values carried G6PD hemizygous mutations. Besides, NGS results revealed an individual (ID 84123) carrying two common SLC22A5 mutations (c.760C>T/p.R254* and c.1400C>G/p.S467C) in Chinese population which were verified in trans later, was biochemical negative in 2016. MS/MS results in June 2019 showed free carnitine deficiency, which was consistence with genetic analysis results. Carrier frequencies data suggested the top five genes with the highest carrier frequencies in these newborns were PAH (2.04%), ETFDH (1.24%), MMACHC (1.15%), SLC25A13 (0.98%), and GCDH (0.80%). Conclusion: Our study provides data that combine biochemical results with genetic variants in 1173 newborns. This is the first study that reveals carrier frequencies of 77 genes causing inherited metabolic diseases (IMDs) in China.
Large-scale sequencing combined with bioinformatics analysis reveals KLF8 an apoptosis repressor in hepatocellular carcinoma
Authors: Xing Hao , Zhang Jin , Zheng Kai-Lian , Wang Ming-Da , Han Jun , Li Chao , Li Zhen-Li , Wu Han , Liang Lei , Wu Meng-Chao , Shen Feng , Yang Tian 
Published: August 2019

Abstract
Backgrounds Hepatocellular carcinoma (HCC) is the most frequent primary liver cancer and the third leading cause of cancer death. Krüppel-like factor 8 (KLF8) is an oncogene and has been shown playing an important role in HCC, but the major involved signaling pathways are still unknown. Here, we systematically analyzed the role of KLF8 in HCC using RNA sequencing and the anti-H3K27 acetylation ChIP sequencing combined with bioinformatics analysis, and the results of data mining. Results The results in this study showed that KLF8 worked as a transcription repressor in HCC and the main directly regulated ones were apoptosis-related genes. Furthermore, we verified the combination of KLF8 with some predicted target genes by ChIP, which supported the effectiveness of our analysis. Besides, we demonstrated that HMGA2 and MMP7, two predicted targets, were important participants in KLF8 mediated anti-apoptotic effect in HCC. Conclusions Our work offers a panoramic view of KLF8’s role in HCC for the first time and facilitates the discovery of new targets for HCC via KLF8.
Evaluation of the bacterial frequency causing ventilator associated pneumonia(VAP) in patients admitted in internal ICUs and internal wards at Shiraz Teaching Hospitals
Authors: Khalili Tahereh , Samadi Nasser , Abadi Milad Shahini Shams , Panah Mohammad Ali Davar , Motamedifar Mohammad , Hadi Nahal 
Published: August 2019

Abstract
Objective

Ventilator associated pneumonia (VAP), which is a subset of hospital infections, occurs in patients who have been mechanically ventilated for at least 48 hours. The aim of this study was to determine the frequency of bacteria causing VAP and determine their antimicrobial susceptibility pattern.Results

The present study is a cross-sectional descriptive study which was conducted in Shiraz during the 8 months (from November 2017 till June 2018). Samples were identified according to the diagnostic tests of bacteriology and antibiotic susceptibility test was performed using CLSI standards. 51 patients with VAP were examined, of which 10 and 8 patient respectively had chronic obstructive pulmonary disease and acute respiratory distress syndrome. The late onset VAP (61.7%) rate was more than compared to the early onset VAP rate (38.3%). The most frequently used antibiotics were meropenem (76.6%) and vancomycin (78.7%). Among 59 bacteria were isolated, Acinetobacter and Pseudomonas were the most prevalent organisms. Our data showed that most of the isolates (40%) belonged to Multi Drug Resistant (MDR) pathogens.
Assessment of knowledge towards breast cancer early detection methods among female health professionals in Adigrat town public health institutions, Tigray, northern Ethiopia, 2018
Authors: Aynalem Amdehiwot , Mebratu Andualem 
Published: August 2019

Abstract
Objective The main objective of the study to determine breast cancer early detection method knowledge among female health professional, Adigrat, town, Tigray, Ethiopia, 2018.Result The study assessed the knowledge of health professionals. Majority of the health professionals 55.7% (60) were founded knowledgeable and 44.3% (48) were not knowledgeable.
ANALYTICAL COMPARISON BETWEEN SPECTROPHOTOMETER AND PORTABLE GLUCOMETER FOR MEASUREMENT OF BLOOD GLUCOSE IN HORSE
Authors: Jima Nanoshe Taye , Megerssa Yoseph Cherinet 
Published: August 2019

Abstract
Objective: Analytical methods comparison for the determination of blood glucose are essential in clinical laboratory practice as it improves the quality of health care through accurate and reliable clinical decision making. Therefore The study was done to assess the analytical performance between point-of-care glucometer and spectrophotometric methods for blood glucose determination.
Results: Twenty paired samples from horses visiting SPANA clinic of college of veterinary medicine and agriculture Addis Ababa University was used. Data obtained from both instruments was compared. The results shows that the mean value of blood glucose concentrations were higher in by the glucometric method (106mg/dl) than by the spectrophotometric (73mg/dl) with the calculated t-statistic significantly different p-value of 0.000. This study showed the clinical inaccuracy of the glucometer over the spectrophotometer.
Influence of Soda Content on Desiccation Cracks in Clayey Soils
Authors: Yang Binbin , Yuan Junhong 
Published: August 2019

Alkaline clayey soil is considered to be a degraded soil, which causes serious issues in engineering and construction projects. The formation and development of desiccation cracks in alkaline clayey soils is a complex process affecting the strength, stability, and permeability of these soils. Therefore, to investigate how soda content can inhibit the development of desiccation cracks in alkaline clayey soils in Ulanhot, Inner Mongolia, China, free desiccation tests were performed to examine the cracks in alkaline clayey soil samples with different soda (Na2CO3) contents in the laboratory. During the cracking process, the changes in water content and the occurrence and evolution of cracks were recorded with a camera. Digital image processing was performed to analyze the structure characteristics of the surface cracks. The results indicated that the characteristics are influenced by the different soda contents. The soda inhibited the development of desiccation cracks in alkaline clayey soils. Three stages were found in the process of desiccation cracking in accordance with the rate of water loss: the residual, normal, and structural shrinkage stages. At <0.4 mol L−1 Na2CO3, the “three straight lines” model could be used to describe the volume shrinkage. However, at >0.4 mol L−1 Na2CO3, the structural shrinkage stage disappeared. Moreover, based on the general soil volume change equation and fractal theory, a model was formulated to quantitatively explain the relationship between the fractal dimension and the volume changes in the soil. The experimental results and equations are important for reducing subgrade deformation and slumps.Core Ideas
Soda content can inhibit the development of desiccation cracks in clayey soils.
There are three stages in the process of desiccation cracking in alkaline clayey soil.
A model is formulated to explain the fractal dimension and the volume changes in the alkaline clayey soil.
The structural shrinkage stage will disappear when Na2CO3 is >0.4 mol L−1.
Pemikiran Abdullah Ahmed An-Naim tentang Dekontruksi Syari’ah sebagai Sebuah Solusi
Authors: Taufiq Ahmad 
Published: August 2019

The laws contained in the Qur'an still exist that require interpretation and have the potential to develop. Muslims worldwide may be entitled to apply Islamic law, provided it does not violate the rights of other people and groups, both within and outside of the Islamic community. That is, in claiming and using individual and collective rights to self-determination, Muslims must also recognize and guarantee equal rights for others. Abdullah, descriptive-analytical nature. Ahmed An-Naim to answer the gap between shari'ah and issues of modernity . Shari'a decontructions are used for Islamic legal issues using the Knife of Analysis used first by Mahmud Thaha's Theory of Law Evolution, the second nasikh mansukh theory, the third Makiyyah and Madaniyah, the fourth the interpretation of the gender-based text, the fifth concept of shari'a on human rights
Manejo do aleitamento materno: ações de enfermeiros das equipes de Estratégias de Saúde da Família de um município do norte do Espírito Santo
Authors: Santos Ludmilla Gripa Barcellos dos , Nascimento Luciana de Cássia Nunes , Leite Franciele Marabotti Costa , Moser Denise Consuelo 
Published: August 2019

Aleitamento Materno; Enfermagem em Saúde Comunitária; Saúde da Criança; Enfermagem.