bluuebunny/crossref_metadata_2025_split huggingface dataset:
48 Impact of weaning strategy on beef cow performance
Authors: Worthington Mary Clarke , Ott Grace C , Poore Matthew H , Pickworth Carrie L 
Published: July 2019

Abstract
It has been shown that physiologic stress in beef cattle can impact animal performance. Weaning stress is recognized as a major stress in a calf’s life. However, less is known of its impacts on the dam or developing fetus, as the dam is at the end of the second trimester at weaning. Therefore, the objective was to investigate the impact of weaning stress on cow performance. A total of 240 cows from two locations were assigned to one of three weaning strategies: abrupt removal of the calf on d 0 (n = 80), d 7 cow-calf fenceline contact (n = 81), and late weaning on d 84 (n = 79). Cows averaged 16 and 24 weeks of gestation on d 0 and d 84 respectively. Weights and BCS were obtained monthly. Behavioral observations occurred over the week before and after weaning for each weaning period. Five late-wean cows per location were milked on d 14 and d 70 to estimate milk yield throughout the late wean period. Data were analyzed using the MIXED procedure of SAS with BW and BCS utilizing repeated measures. Means are declared different at P ≤ 0.05. The ADG over the course of the study was lower (P < 0.05) for the late weaned cows compared to the abrupt weaned cows. This was also reflected by change in body condition where late weaned cows dropped from a 5.7 to a 4.9 (P < 0.05) over the 105 d. Milk production in late weaned cows decreased over time (6.0 and 4.4 kg/d for d 14 and 70, respectively; P = 0.05). Energy expenditure for lactation may have contributed to the drop in BCS of the late weaned compared with the non-lactating cows and could compromise third trimester growth of calf in utero. The results of this study warrant further exploration of the effect of weaning stress on dams and their developing fetuses.
46 Species-specificity in metmyoglobin reduction
Authors: Bechtold Emily , Suman Surendranath , Mohanty Smita , Mazumder Suman , Krishnan Sadagopan , Nerimetla Raja 
Published: July 2019

Abstract
Myoglobin is the primary sarcoplasmic protein responsible for meat color. Previous research has reported that myoglobin oxidation is species-specific. Metmyoglobin reducing activity is an inherent property to limit myoglobin oxidation. However, limited research has determined species specificity in metmyoglobin reducing properties. The objective of current study was to compare metmyoglobin reducing properties of eight different species such as beef, porcine, bison, deer, emu, equine, goat, and sheep in vitro. Myoglobin was isolated from eight different species via ammonium sulfate precipitation. The pH of the myoglobin was adjusted by passing through a column pre-calibrated with 50 mM phosphate buffer at pH 5.6. All species myoglobin were converted to metmyoglobin, and the metmyoglobin reduction was determined by two different approaches, non-enzymatic metmyoglobin reducing activity (NMRA) and oxidation-reduction potential (ORP). In the first method, NADH (electron donor), EDTA, and methylene blue (electron carrier), were added in a cuvette and increase in absorbance at 582 nm was monitored using a UV-Vis spectrophotometer. In the second method, the ability of the heme to get reduced was determined using an RedoxSys analyzer, in which electron was directly transferred to heme. The NMRA and ORP experiments were replicated five times. The data were analyzed using the Mixed Procedure of SAS, with species as the fixed effect. There were species-specific differences (P < 0.05) in NMRA and ORP. Bovine myoglobin had the greatest (P < 0.05) NMRA compared with sheep, equine, goat, deer, bison, pork, and emu. There were no differences (P > 0.05) in NMRA among equine, goat, deer, bison, pork, and emu. ORP studies indicated that beef and porcine myoglobins had the greatest ability to get reduced (P < 0.05) compared with other species. Hence, use of different techniques and approaches will help to elucidate the mechanistic basis of metmyoglobin reduction.
54 Digestibility of forages in ruminally cannulated Jersey steers supplemented with liquid glycerol on a wheat straw forage diet
Authors: Soto Itzel A , Boyd Jamie 
Published: July 2019

Abstract
The purpose of this study was to determine the impact of supplementation of glycerol at a rate of 15% of DMI on forage digestibility on a diet of mature wheat hay. Forages tested included bermuda hay, wheat hay, alfalfa hay, and sorghum sudan hay. Samples were ground through a 2mm screen and placed in Ankom forage in-situ bags. Samples were run in triplicate to reduce error and increase statistical significance. Two ruminally cannulated steers were used in a 2x2 Latin square design for the study with ad libitum access to wheat hay and water. Animals were housed in a dry lot pen for the study. Diets offered were Control = wheat hay and Treatment= control + glycerol at 15% of daily DMI. The study consisted of 2 three-week periods. Each period had a 2-week standardization followed by a 1-week test period. During the test period, forage bags were placed in each steer for 0 h, 48 h, 72 h, 96 h, and 144 h for fermentation. At the end of the incubation period, bags were collected and cold shocked to stop microbial digestion. The samples were then dried at 55°C and stored for later analysis for NDF. A significant impact on NDFdig by treatment (P < 0.0001) was observed. NDFdig was 59.2% w/ glycerol versus control at 49.13% overall. In addition, there was a significant difference between steers (P = 0.0001) on glycerol treatment (51.95% versus 66.45% NDFdig between steer A and B), indicating difference in ruminal population efficiency between individual animals. No significant difference (P = 0.98) between steers on the control treatment was observed. Implications of this study are the inclusion of dietary glycerol in the diet of ruminants on a low-quality forage diet may increase energy level and improve ruminal digestibility and animal response may be dependent on the unique ruminal environment of individual animals.
52 Timing of progesterone exposure effects pregnancy rates following fixed timed artificial insemination in postpartum beef cows
Authors: Smith Anna L , Poole Rebecca K , Mayberry Kyle , Newsome McKayla A , Dudley Harrison B , Poole Daniel H 
Published: July 2019

Abstract
Advancements in reproductive technologies have improved pregnancy rates in postpartum cows; however, undesirable consequences leading to reduced fertility still occur. Thus, the objectives were to determine if presence of a corpus luteum (CL) at the start of estrous synchronization (AIM1) or if the length of proestrus (interval from progesterone removal to a GnRH-induced LH surge; AIM2) alters fixed timed artificial insemination (FTAI) pregnancy rates. Cycling postpartum cows (n = 285) at two locations were synchronized using the CO-Synch+CIDR® FTAI protocol. Ultrasonographic measurements of luteal area were recorded at CIDR insertion. To determine the impact of proestrus length, cows were randomly assigned to either undergo CIDR removal at the time of PGF2a injection (long proestrus, n = 145), or CIDR removal 24 hours post PGF2a injection (short proestrus, n = 140). Blood samples were collected at CIDR insertion and at insemination for progesterone (P4) analysis. Pregnancy status to FTAI was assessed using ultrasonographic diagnosis at 30 d post insemination. Data were analyzed using a MIXED procedure of SAS and examined for effects of length of proestrus, P4 concentration, luteal area, sire, location, and age. Statistical significance was determined at P 0.05) pregnancy rates. Pregnancy rates were greater in cows with >1 ng/mL P4 compared to cows with 0.05). Cows > 4 y old had higher AI pregnancy rates compared to 2–3 y cows (70.2±7.4 vs. 51±5.8%, respectively; P > 0.05). Based on these data, incorporating a presynchronization program to ensure the presence of a functional CL at the start of a FTAI protocol may improve pregnancy success in postpartum beef cows.
5 Effect of feeding time on behavioral and stress responses in gestation sows under isocaloric conditions
Authors: Manu Hayford , Lee S H , Huang Q , Pangeni D , Keyes M C , Cairns J , Baidoo S K 
Published: July 2019

Abstract
Investigated the effect of feeding time on behavior and stress responses in pregnant sows under isocaloric conditions. Eighteen sows (Landrace × Yorkshire); BW 248.6 ± 2.8 kg; parity 3.30 ± 0.39); were blocked by parity and randomly assigned to 1 of 3 feeding times. Corn-soybean meal-based diet was fed once at: 0730 (Control, T1), 1130 (T2), and 1530 h (T3). On average, sows received 7190 kcal ME d−1 during gestation from 2.25 kg of diet formulated to contain SID Lys/ME of 1.71 g/Mcal. Saliva samples were collected every 2 h for 12 h in stalls on d 52 of pregnancy and assayed for cortisol using ELISA technique. Behavior data were collected 24 h for 7 d from d 53 of gestation by affixing a Remote Insights ear tag to each sow after 21 d adaptation period. Each sow had 120,960 data points categorized into: “Active,” “Feed” or “Dormant”. Data were analyzed using GLIMMIX procedure of SAS 9.4. Statistical significance was set at P ≤ 0.05, and a trend as 0.05 < P ≤ 0.10. Sow was the experimental unit. A 24 h area under the curve (AUC) was estimated by trapezoidal summation method. Adjustment for multiple comparisons was based on Tukey Kramer’s method. Sows fed daily at 0730 had both lower feeding and total activity compared with sows fed at 1130 h (P < 0.02) but similar to sows fed at 1530 h (P > 0.05). Feeding sows at 1130 h daily resulted in reduced cortisol AUC compared with 1530 h fed sows (P < 0.04) but similar to sows fed at 0730 h (P > 0.05). In conclusion, feeding sows daily at 1130 resulted in increased feed and total activity but reduced cortisol concentration, suggesting that elevated sow activity might not necessary indicate activation of hypothalamic-pituitary-adrenal (HPA) axis.
11 Use of pressure mat gait analysis in measuring pain following normal parturition in dairy cows
Authors: Kleinhenz Michael , Gorden Patrick , Burchard Michael , Ydstie Joshua , Coetzee Johann 
Published: July 2019

Abstract
Research investigating the pain of normal parturition is lacking as there are few objective methods for measuring pain. The objective of this research was to describe the gait of cows following eutocia using pressure-mat gait analysis; and determine if meloxicam alters the gait of cows. Twenty Holstein cows within 26 h of unassisted calving were enrolled into the study. Treatment groups included: 1) post-partum cows administered meloxicam (MEL; n = 10); and 2) post-partum cows administered placebo (PLBO; n = 10). Meloxicam was administered by oral bolus at 1 mg/kg within 26 hours of calving. Placebo cows were given an oral bolus of dry whey powder within 26 hours of calving. A commercially available floor mat-based pressure/force measurement system was used to compare ambulation between treatment groups. Cows were walked across the mat before treatment administration, and 2, 4, 6, 8, 12, 24, 48 and 72 hrs post-treatment. The percent of total force, percent total contact pressure, and percent total impulse of the rear limbs was calculated. Outcome measures were statistically analyzed using repeated measures, with the cow serving as the experimental unit. Cows in the MEL group placed 48.9% (95% CI: 47.4 – 50.5%) of total force on the rear limbs compared to 46.3% (95% CI: 44.7 – 47.9%) in PLBO cows (P = 0.02). Total impulse on their rear limbs for the MEL cows was 50.5% (95% CI: 48.6 – 52.4%) compared to 46.7% (95% CI: 44.8 – 48.7%) for the PLBO cows (P = 0.01). No differences in contact pressure of the rear limbs was observed (P = 0.27). The PLBO cows had a longer gait 101.3 cm (95% CI: 95.9 – 106.6 cm) versus 90.8 cm (95% CI: 85.4 – 96.1 cm) (P = 0.03). These findings show meloxicam treated cows have altered weight distribution to the rear limbs as measured by pressure mat gait analysis, suggesting meloxicam may effectively treat post-partum pain.
12 A multimodal approach for piglet pain management after tail docking
Authors: Viscardi Abbie V , Turner Patricia 
Published: July 2019

Abstract
Millions of piglets on commercial production farms are tail docked each year to reduce the incidence of tail biting. While this procedure is known to be painful, piglets in the United States are often not provided analgesia or anesthesia for pain relief. The objectives of this study were to assess a multimodal approach to managing tail docking pain in piglets, using 0.4 mg/kg meloxicam (MEL), 0.04 mg/kg buprenorphine (BUP), and Maxilene® (MAX). The effectiveness of each drug and drug combination was evaluated using behavioral indicators, vocalization, and facial grimace analysis. This study also assessed whether male and female piglets responded differently to pain or pain treatments. Four-day-old piglets from 14 litters (n = 165 total) were used and piglets within a litter were randomly assigned to one of six possible treatments: MEL, BUP, MEL + BUP, MEL + BUP + MAX, no treatment (tail docked control), or sham (non-tail docked control). Treatments were administered intramuscularly (MEL, BUP) or topically on the tail (MAX) 20 min prior to tail docking. Piglets administered MEL + BUP and BUP demonstrated significantly fewer pain behaviors than piglets in the MEL and no treatment group (p < 0.05). MEL + BUP + MAX and BUP piglets also grimaced significantly less than piglets in the no treatment group (p < 0.05). There were no significant differences in emitted vocalizations between the analgesia-treated piglets and the no treatment group, and both injection and tail docking elicited piglet vocalizations of similar frequency, power, and energy (P > 0.05). There were no significant differences in behavior, facial grimacing or emitted vocalizations between male and female piglets. All treatment groups with buprenorphine were able to alleviate tail docking-associated pain, suggesting that opioid administration is highly effective for managing piglet pain.
13 Effect of housing cow-calf pairs on dry lots vs pasture on calf performance and behavior
Authors: Neira Lucas T , Cooney Ashley E , Chapple Wesley P , Meteer William T , Shike Daniel W 
Published: July 2019

Abstract
The objectives were to analyze the effects of housing cow-calf pairs in dry lots (DL) or pasture (PAST) on calf performance and behavior through the receiving phase. Simmental × Angus (n = 108; 87 ± 11.5 d postpartum) spring-calving cows were stratified by age, BW, BCS, and calf sex and allotted to six groups. Cows were limit-fed a ration consisting of corn silage, dried distillers grain, corn stalks, dry rolled corn (DRC), and soybean hulls to meet protein and energy requirements. Calves had ad libitum access to same diet in an adjacent pen. Calves on PAST were rotationally grazed with free-choice mineral. After weaning (177 ± 11.5 d of age) all calves were fed a diet consisting of corn silage, wet distiller grains, DRC, and grass hay during the receiving phase (42 d). At the age 87, 142, 177, 198, and 219 ± 11.5 d, BW was measured. Hair coat score (HCS) and dirt score (DS) were measured on d 0 and d 90. Calf behavior was observed after feedlot arrival. Average daily gain, DMI and feed efficiency were also analyzed. The data were analyzed using the MIXED procedure of SAS. Dry-lot calves had greater (P < 0.01) BW and ADG prior to weaning. Calves from PAST had lower (P < 0.01) DS and greater (P < 0.01) HCS at weaning. Upon feedlot arrival, more (P < 0.01) DL calves were walking and had increased (P < 0.01) vocalizations. Calves from PAST had greater (P < 0.01) ADG, DMI as a percent of BW, and gain:feed than DL calves during the receiving phase. Calves raised in a dry lot had greater BW and ADG prior to weaning, but PAST calves had fewer behavioral signs of stress and greater growth performance in feedlot.
21 Issues of sow lifetime productivity from an international perspective: from research to production level
Authors: Bortolozzo Fernando , Ulguim Rafael , Mellagi Ana Paula , Mallmann Andre L , Bernardi Mari 
Published: July 2019

Abstract
Sow longevity is defined as the time elapsed from gilt entry into the breeding herd until her removal as a result of culling or death. Increasing herd retention rate is important to improve sow lifetime productivity. Higher removal rate implies an increase in the percentage of young females in the herd, a category with lower production potential and a higher risk of early culling. Systematic gilt replacement is required for genetic gain, but it is essential to achieve a retention rate that allows the sows to reach their maximum production potential in the later parities. In this way, it is important to evaluate the reasons for removal and death in order to establish strategies for reducing involuntary culling and controlling mortality rate. Although reproductive failures persist as the main culling cause, its expressivity has been apparently reduced. To improve sow retention rate, management intervention should start during gestation with a focus on improving the replacement gilt’s weight at birth. Ensuring adequate colostrum intake is critical for achieving targeted pre-wean and post-wean growth rate. In addition, approaches that improve gilt body development during the post-weaning phase and up to first breeding are key steps to reducing culling for structural problems and low reproductive performance. In this sense, the use of gilt development units allows a regulated flow of replacement gilts that meet the required parameters recommended by the different genetic suppliers. Because locomotor disorders are frequent reasons for early culling, inspection and maintenance of the animal facilities should be prioritized in all phases of rearing gilts and sows. The opportunity to reduce sow mortality, culling for poor reproduction, and structural failure resides in the ability to translate new research discoveries on gilt birthweight, growth, and body measures into improved lifetime pig production to the 3rd parity.
19 Overview of the National Pork Board replacement gilt feet and leg evaluation trial
Authors: Stock Joseph D , Supakorn China , Calderon Diaz Julia A , Rothschild Max F , Stalder Kenneth J 
Published: July 2019

Abstract
Replacement gilt feet and leg conformation traits are subjectively evaluated at early ages (150 d) in order to select acceptable replacements. The objectives of this study were to: 1) evaluate replacement gilt feet and leg conformation (FLC) using subjective and objective measurement methods, 2) estimate objective joint angle changes over two parities, and 3) evaluate the effect of three diets differing in standardized ileal digestible (SID) lysine concentrations on FLC traits using the objective evaluation method. Crossbred Large White × Landrace gilts (n = 641) were used. Gilts were moved to GDU at 77 days-of-age and randomly allotted to three ad libitum fed dietary treatments providing similar ME but differing in SID lysine concentrations (low, medium, and high) during the grow-finisher period. All animals received common gestation and lactation diets. Feet and leg conformation traits were evaluated at 100 d, 200 d, 1st litter (392 ± 25 d) and 2nd litter (548 ± 49 d) weaning events. Subjective evaluation was done on a scale from 1 to 5. Data were analyzed using mixed linear model methods. The majority of gilts received the intermediate score (i.e. 3) when joint angles were subjectively evaluated. No differences (P ≥ 0.05) were observed for knee, front pastern and hock objective joint angles between subjective scores assigned. Differences in subjectively evaluated rear pastern (P < 0.05) were observed, where objective joint angles associated with scores of ≤ 2 and 3 were lower than score of ≥ 4. There was no interaction between dietary treatment and objective evaluation time-period for any FLC traits studied (P > 0.05). Females with greater knee, hock, front and rear pastern objective joint angles at 100 d of age, continued to have greater joint angles (0.2 ± 0.04 degrees; P < 0.001) later in life. Front and rear pastern joint angles decreased (P < 0.001) and hock joint angles increased from 100 d to end of study (P < 0.001).
32 Functional annotation of livestock genomes: chromatin structure and regulation of gene expression
Authors: Foissac Sylvain , Djebali Sarah , Munyard Kylie , Vialaneix Nathalie , Rau Andrea , Acloque Herve , Lagarrigue Sandrine , Giuffra Elisabetta 
Published: July 2019

Abstract
Improving the functional annotation of animal genomes is a key challenge in bridging the gap between genotype and phenotype, thus enabling predictive biology. Regarding livestock production, major outcomes are expected from a better understanding of the genetic architecture underlying quantitative traits. As part of the Functional Annotation of ANimal Genomes action (FAANG: www.faang.org), the FR-AgENCODE project generated omics data to improve the reference annotation of the cattle, pig, goat and chicken genome. High-throughput molecular assays have been performed on tissues/cells relevant to immune and metabolic traits. From two males and two females per species (pig, cattle, goat, chicken), strand-oriented RNA-seq gene expression and ATAC-seq chromatin accessibility assays were performed on liver and two PBMC-sorted T-cell types (CD4+ and CD8+). Chromosome Conformation Capture (in situ Hi-C) was also carried out on liver samples. About 4,000 samples have been collected at the INRA biorepository and registered at the EBI BioSamples registry. More than 80% of the planned experiments could be completed, generating ~11.5 billions of sequencing reads over the 3 assays. While most (50–80%) RNA-seq reads mapped to annotated exons, thousands of novel transcripts were found, with ~60K mRNAs and ~22K lncRNAs in cattle. Differentially expressed genes between cell types were enriched for immunity- or metabolism-related terms, and differentially accessible chromatin regions were identified as potential regulatory sites. Interestingly, correlations between gene expression and promoter accessibility across samples were skewed towards both positive and negative values, suggesting distinct regulatory mechanisms of gene expression. These patterns have been further investigated using human data from the Epigenome Roadmap Mapping Consortium. Altogether, this study illustrates the interest of a coordinated effort to tackle the genome-to-phenome challenge and provides a useful resource to the community. Availability: www.fragencode.org.