bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oncogenic role of phospholipase C‐γ1 in progression of hepatocellular carcinoma
Authors: Tang Wenqing , Zhou Yi , Sun Dalong , Dong Ling , Xia Jinglin , Yang Biwei 
Published: January 2019

AimPhospholipase C‐γ1 (PLCG1) was previously found to be involved in a variety of oncogenic behaviors such as cell motility, cell proliferation, cell migration, and invasion. However, its function in hepatocellular carcinoma (HCC) was unknown. Here, we explored the expression pattern and function of PLCG1 in HCC progression.MethodsExpression of PLCG1 was examined by western blotting in hepatoma cells and human tumor tissues. Expression was also detected by immunohistochemistry in 150 HCC clinical samples, and its clinical significance was analyzed. The influence of PLCG1 on HCC carcinogenesis were determined in vitro and in vivo. The underlying mechanisms were explored by detecting the expression of critical molecules of signaling pathways.ResultsThe results showed that PLCG1 was overexpressed in hepatoma cell lines and clinical HCC tissues. Increased PLCG1 expression in tumor tissues was remarkably correlated with poor clinical features of HCC. Patients with positive PLCG1 expression in tumor tissues had shorter overall survival and relapse‐free survival. Phospholipase C gamma 1 could substantially promote cell proliferation, anchor growth, and cell invasion in vitro. The in vivo study showed that inhibition of PLCG1 in hepatoma cells significantly repressed tumor growth in nude mice. Furthermore, we showed that PLCG1 might exert its function by activating the mitogen‐activated protein kinase and nuclear factor‐κB signaling pathways.ConclusionOur data indicated that PLCG1 could act as an oncogene in HCC carcinogenesis and could serve as a valuable prognostic marker and potential therapeutic target for HCC.
Biocompatibility and biomineralization ability of Bio‐C Pulpecto. A histological and immunohistochemical study
Authors: Cosme‐Silva Leopoldo , Benetti Francine , Dal‐Fabbro Renan , Gomes Filho João Eduardo , Sakai Vivien T. , Cintra Luciano T. A. , Alvarez Nathalia , Ervolino Edilson , Viola Naiana V. 
Published: January 2019

AimTo evaluate the inflammatory response, biomineralization and production of osteocalcin (OCN), osteopontin (OPN), and bone sialoprotein (BSP) of a new root filling material for primary teeth (Bio‐C Pulpecto) compared to MTA.DesignPolyethylene tubes containing Bio‐C Pulpecto, MTA, and empty tubes were implanted into the dorsal connective tissue. After 7, 15, 30, 60, and 90 days, the tubes with surrounding tissue were removed and histologically processed to be analysed using haematoxylin and eosin, von Kossa staining, or no staining for observation under polarized light and immunohistochemistry for the detection of OCN, OPN, and BSP. The Kruskal‐Wallis test was used followed by Dunn's test. The significance level was set at 5%.ResultsThe inflammatory response observed with MTA and Bio‐C Pulpecto was more exacerbated until the 15th day and decreased from 30 days on. No significant difference was found between control, MTA, and Bio‐C Pulpecto (P > 0.05). Bio‐C Pulpecto and MTA showed positivity for von Kossa and birefringent to polarized light. The immunolabelling for OCN, OPN, and BSP was more intense for MTA and Bio‐C Pulpecto on days 60 and 90 (P > 0.05).ConclusionBio‐C Pulpecto was biocompatible, induced biomineralization and was immunopositive for osteogenic markers such as OCN, OPN, and BSP, similarly to MTA.
Evaluating the effects of multisystemic therapy for adolescents with intellectual disabilities and antisocial or delinquent behaviour and their parents
Authors: Blankestein Annemarieke , van der Rijken Rachel , Eeren Hester V. , Lange Aurelie , Scholte Ron , Moonen Xavier , De Vuyst Katrien , Leunissen Jo , Didden Robert 
Published: January 2019

AbstractBackgroundAn adaptation of multisystemic therapy (MST) was piloted to find out whether it would yield better outcomes than standard MST in families where the adolescent not only shows antisocial or delinquent behaviour, but also has an intellectual disability.MethodTo establish the comparative effectiveness of MST‐ID (n = 55) versus standard MST (n = 73), treatment outcomes were compared at the end of treatment and at 6‐month follow‐up. Pre‐treatment differences were controlled for using the propensity score method.ResultsMultisystemic therapy‐ID resulted in reduced police contact and reduced rule breaking behaviour that lasted up to 6 months post‐treatment. Compared to standard MST, MST‐ID more frequently resulted in improvements in parenting skills, family relations, social support, involvement with pro‐social peers and sustained positive behavioural changes. At follow‐up, more adolescents who had received MST‐ID were still living at home.ConclusionsThese results support further development of and research into the MST‐ID adaptation.
MicroRNAs mediate liver transcriptome changes upon soy diet intervention in mice
Authors: Seclaman Edward , Balacescu Loredana , Balacescu Ovidiu , Bejinar Cristina , Udrescu Mihai , Marian Catalin , Sirbu Ioan Ovidiu , Anghel Andrei 
Published: January 2019

AbstractSoy‐based diets have triggered the interest of the research community due to their beneficial effects on a wide variety of pathologies like breast and prostate cancer, diabetes and atherosclerosis. However, the molecular details underlying these effects are far from being completely understood; several recent attempts have been made to elucidate the soy‐induced liver transcriptome changes in different animal models. Here we used Next Generation Sequencing to identify a set of microRNAs specifically modulated by short‐term soy‐enriched diet in young male mice and estimated their impact on the liver transcriptome assessed by microarray. Clustering and topological community detection (CTCD) network analysis of STRING generated interactions of transcriptome data led to the identification of four topological communities of genes characteristically altered and putatively targeted by microRNAs upon soy diet intervention.
Efficacy of lateral bone augmentation prior to implant placement: A systematic review and meta‐analysis
Authors: Naenni Nadja , Lim Hyun‐Chang , Papageorgiou Spyridon N. , Hämmerle Christoph H. F. 
Published: January 2019

AbstractAimThe aim of the current systematic review was to critically appraise evidence from randomized and prospective non‐randomized comparative clinical trials about the efficacy of lateral bone augmentation prior to implant placement and their outcome regarding bone width gain.Materials and MethodsEight databases were searched until May 2018 for randomized and prospective non‐randomized comparative trials on lateral bone augmentation prior to implant placement. After elimination of duplicate studies, data extraction and risk‐of‐bias assessment according to the Cochrane guidelines, random‐effects meta‐analyses of mean differences (MD) or relative risks (RR) and their 95% CIs were performed, followed by subgroup, meta‐regression and sensitivity analyses.ResultsOverall, 25 trials (16 randomized/9 non‐randomized) were identified, which included a total of 553 patients (42.2% male; mean age of 43.9 years). In these included studies and populations, various modalities for primary lateral bone augmentation rendered implant placement feasible. Small discrepancies were found between overall clinical and radiographic gain (pooled gains of 3.45 ± 1.18 mm versus 2.90 ± 0.83 mm, respectively), but were not statistically significant. Bone width gain was significantly inversely associated with baseline bone width (pooled effect: −0.35 mm/mm; 95% CI: −0.63 to −0.07 mm; p = 0.01). Additionally, % graft resorption was associated with patient age (36%/year, 95% CI: −0.62 to −0.11 mm; p = 0.01). The presence of xenograft added to autologous graft led to less resorption compared to autologous graft alone (MD: 1.06 mm; 95% CI: 0.21 to 1.92 mm; p = 0.01). Barrier membrane did not yield significant difference in terms of bone width gain (MD: −0.33 mm; 95% CI: −2.24 to 1.58 mm; p > 0.05) and graft resorption (MD: 0.84 mm; 95% CI: −1.42 to 3.09 mm; p > 0.05). However, the quality of evidence ranged from very low to moderate due to bias and imprecision.ConclusionsInitially smaller bone dimensions are associated with favours larger bone width gain, which indicates that a severe lateral bone deficiency can be effectively augmented applying primary lateral bone augmentation. Both Patients’ age and recipient site (maxilla or mandible) seem to influence graft resorption. The addition of a xenograft can be helpful in reducing graft resorption. Existing evidence from randomized and prospective non‐randomized trials on humans indicates that lateral bone augmentation prior to implant placement can successfully increase bone width. There are some indications that patient‐related, site‐related, and technique‐related characteristics might influence the amount of gained bone width, but the quality of evidence is for the most part hampered by the small number of existing studies and methodological limitations that might lead to bias.
Mesenchymal stem cells and biologic factors leading to bone formation
Authors: Bartold Mark , Gronthos Stan , Haynes David , Ivanovski Saso 
Published: January 2019

AbstractBackgroundPhysiological bone formation and bone regeneration occurring during bone repair can be considered distinct but similar processes. Mesenchymal stem cells (MSC) and associated biologic factors are crucial to both bone formation and bone regeneration.AimTo perform a narrative review of the current literature regarding the role of MSC and biologic factors in bone formation with the aim of discussing the clinical relevance of in vitro and in vivo animal studies.MethodsThe literature was searched for studies on MSC and biologic factors associated with the formation of bone in the mandible and maxilla. The search specifically targeted studies on key aspects of how stem cells and biologic factors are important in bone formation and how this might be relevant to bone regeneration. The results are summarized in a narrative review format.ResultsDifferent types of MSC and many biologic factors are associated with bone formation in the maxilla and mandible.ConclusionBone formation and regeneration involve very complex and highly regulated cellular and molecular processes. By studying these processes, new clinical opportunities will arise for therapeutic bone regenerative treatments.
Contributions of climate, plant phenology and natural enemies to the seasonal variation of aphids on cabbage
Authors: Fidelis Elisangela G. , Farias Elizeu S. , Lopes Mayara C. , Sousa Fernanda F. , Zanuncio José C. , Picanço Marcelo C. 
Published: January 2019

AbstractBrevicoryne brassicae (L), Lipaphis erysimi (Kalt) and Myzus persicae (Sultzer) (Homoptera: Aphididae) form the aphid complex that causes great losses in Brassicaceae in tropical and subtropical regions. Knowledge of their population dynamics is important for the development of integrated pest management programmes. This study aimed to investigate the effects of cabbage phenology, climatic factors and natural enemies populations on the dynamics of these organisms, and the factors regulating their predators’ occurrence. The densities of aphids and their natural enemies and the climate were monitored for two years in 16 cabbage crops. The highest densities of the aphids occurred during periods of relative humidity (RH) drop, a condition that affects them positively. Regarding the predators, the factors affecting their abundance varied but RH was positively related to most of them. This study provides relevant insights into the factors that regulate the aphids in cabbage and for the decision‐making process of control of these severe pests.
Primary Care Management of Upper Respiratory Infections in the Women's Health Care Setting
Authors: Levi Miriam E. 
Published: January 2019

AbstractUpper respiratory infections (URIs) are the most common reason for which individuals seek health care services in the outpatient clinical setting. This case report describes the clinical presentation and management of a woman with a URI. The signs and symptoms of URIs, physical examination, differential diagnoses, and treatment plan options available in the ambulatory care setting are reviewed. Current evidence‐based guidelines are discussed, and recommendations for clinical practice are reviewed. Discussion of the incidental treatment of URIs in the specialty care setting is also addressed.
Dental care utilization: patterns and predictors in persons living with HIV in British Columbia, Canada
Authors: Jessani Abbas , Aleksejuniene Jolanta , Donnelly Leeann , Craig Phillips J. , Nicolau Belinda , Brondani Mario 
Published: January 2019

AbstractObjectivesTo identify the predisposing, enabling, and need factors of the Andersen and Newman (A&N) model and their associations with the pattern of dental service utilization in a sample of people living with HIV (PLHIV) in British Columbia.MethodsParticipants responded anonymously to a 40‐item online questionnaire to explore the patterns of dental service utilization. Following the descriptive statistics, the associations between A&N model factors and main outcome variables (having a dental visit in the last year and reasons for the dental visit) were evaluated using simple and multiple logistic regression analyses.ResultsOut of 600 potential PLHIV participants, 210 responded to the survey and 186 met the inclusion criteria. The experience of being discriminated against by dental professionals (P = 0.005), having dental anxiety (P < 0.001), not having dental insurance (P = 0.001), and having living condition difficulties (P = 0.004) were significantly associated with nonemergency dental visits. In multiple logistic regression analysis, dental anxiety (OR = 0.1; 95 percent CI 0.0; 0.4), having a regular dentist (OR = 3.7; 95 percent CI 1.1; 12.6), and visiting a dental office in the last year (OR = 21.6; 95 percent CI 6.1; 76.5) were the strongest predictors of dental service utilization in this study.ConclusionsSeveral predisposing, enabling, and need factors from the A&N model were associated with dental service utilization by PLHIV. In addition to various psychosocial barriers, a significant number of respondents reported experiencing stigma and discrimination from their oral care providers.
Social cognition, social neuroscience, and evolutionary social psychology: What's missing?
Authors: Greenwood John D. 
Published: January 2019

AbstractIn this paper I argue that something important, and something social, is missing from contemporary accounts of social cognition, social neuroscience and evolutionary social psychology. Contemporary accounts of social cognition focus on cognition directed towards social objects, that is, towards persons and social groups. In contrast, early twentieth century accounts of socially engaged cognition focused upon beliefs and attitudes oriented to the represented beliefs and attitudes of members of social ‘reference groups’ and directed towards both social and non‐social objects. I argue that this earlier conception of socially engaged cognition should be integrated with contemporary research on social cognition, social neuroscience and evolutionary social psychology, since it poses a challenge but also an opportunity for these disciplines.
Prevalence of the E321GMYH1variant for immune‐mediated myositis and nonexertional rhabdomyolysis in performance subgroups of American Quarter Horses
Authors: Gianino Giuliana M. , Valberg Stephanie J. , Perumbakkam Sudeep , Henry Marisa L. , Gardner Keri , Penedo Cecilia , Finno Carrie J. 
Published: January 2019

BackgroundImmune‐mediated myositis (IMM) in American Quarter Horses (QHs) causes acute muscle atrophy and lymphocytic infiltration of myofibers. Recently, an E321G mutation in a highly conserved region of the myosin heavy chain 1 (MYH1) gene was associated with susceptibility to IMM and nonexertional rhabdomyolysis.ObjectivesTo estimate prevalence of the E321GMYH1variant in the QH breed and performance subgroups.AnimalsThree‐hundred seven elite performance QHs and 146 random registered QH controls.MethodsProspective genetic survey. Elite QHs from barrel racing, cutting, halter, racing, reining, Western Pleasure, and working cow disciplines and randomly selected registered QHs were genotyped for the E321GMYH1variant and allele frequencies were calculated.ResultsThe E321GMYH1variant allele frequency was 0.034 ± 0.011 in the general QH population (6.8% of individuals in the breed) and the highest among the reining (0.135 ± 0.040; 24.3% of reiners), working cow (0.085 ± 0.031), and halter (0.080 ± 0.027) performance subgroups. The E321GMYH1variant was present in cutting (0.044 ± 0.022) and Western Pleasure (0.021 ± 0.015) QHs at lower frequency and was not observed in barrel racing or racing QHs.Conclusions and Clinical ImportanceKnowing that reining and working cow QHs have the highest prevalence of the E321GMYH1variant and that the variant is more prevalent than the alleles for hereditary equine regional dermal asthenia and hyperkalemic periodic paralysis in the general QH population will guide the use of genetic testing for diagnostic and breeding purposes.
The Effect of Hearing Impairments on Wage Earnings: Evidence from a Copula‐Based Spline Selection Model
Authors: Zimmer David M. 
Published: January 2019

AbstractThis paper investigates the effect of hearing impairments on annual wage earnings. A standard Heckman‐style selection model suggests that hearing impairments reduce earnings by approximately 14 per cent, with no evidence of selection bias. The paper then relaxes the assumption of bivariate normality using a copula specification, and the paper allows for potentially nonlinear links between nonbinary covariates and earnings using smooth spline functions. By incorporating those two deviations, not only does the model appear to provide a better fit to the data, but hearing impairments appear to reduce earnings by 19 per cent, an effect 36 per cent larger than what is suggested by the standard selection setup. The model also uncovers evidence of statistically significant selection bias.
Molecular insights into the master regulator CysB‐mediated bacterial virulence in Pseudomonas aeruginosa
Authors: Song Yaqin , Yang Chun , Chen Gukui , Zhang Yixi , Seng Zijing , Cai Zhao , Zhang Chao , Yang Liang , Gan Jianhua , Liang Haihua 
Published: January 2019

SummaryPseudomonas aeruginosa is a major pathogen that causes serious acute and chronic infections in humans. The type III secretion system (T3SS) is an important virulence factor that plays essential roles in acute infections. However, the regulatory mechanisms of T3SS are not fully understood. In this study, we found that the deletion of cysB reduced the T3SS gene expression and swarming motility but enhanced biofilm formation. In a mouse acute pneumonia model, mutation of cysB decreased the average bacterial load compared to that of the wild‐type strain. Further experiments demonstrated that CysB contributed to the reduced T3SS gene expression and bacterial pathogenesis by directly regulating the sensor kinase RetS. We also performed crystallographic studies of PaCysB. The overall fold of PaCysB NTD domain is similar to other LysR superfamily proteins and structural superposition revealed one possible DNA‐binding model for PaCysB. Structural comparison also revealed great flexibility of the PaCysB RD domain, which may play an important role in bending and transcriptional regulation of target DNA. Taken together, these results expand our current understanding of the complex regulatory networks of T3SS and RetS pathways. The crystal structure of CysB provides new insights for studying the function of its homologs in other bacterial species.
A systematic review of nurse‐led weaning protocol for mechanically ventilated adult patients
Authors: Hirzallah Fatima Mohammad , Alkaissi Aidah , do Céu Barbieri‐Figueiredo Maria 
Published: January 2019

ABSTRACTObjectivesThe aim of this systematic review is to synthesize the current best evidence for the effectiveness of weaning protocols led by nurses compared with usual physician‐led care.BackgroundProtocol‐directed weaning has been shown to reduce the duration of mechanical ventilation. Studies have reported that a weaning protocol administered by nurses leads to a reduction in the duration of mechanical ventilation and has a major effect on weaning outcomes. This can have especially positive consequences for critically ill patients.Study designSystematic review with meta‐analysis.Search strategyThe databases CINAHL, PubMed, Scopus, and the Cochrane Central Register of Controlled Trials were searched from as far back as the database allowed until January 2016.Inclusion and exclusion criteriaSearches were performed to identify the best available evidence including quantitative studies of nurse‐led weaning protocols for mechanically ventilated adult patients. We excluded all studies of weaning protocols implemented by non‐nurses and non‐invasive mechanical ventilation and studies that addressed patient populations younger than 18 years of age.ResultsThe database searches resulted in retrieving 369 articles. Three eligible studies with a total of 532 patients were included in the final review. Pooled data showed a statistically significant difference in favour of the nurse‐led weaning protocol for reducing the duration of mechanical ventilation (mean differences = −1.69 days, 95% confidence interval = −3.23 to 0.16), intensive care unit length of stay (mean differences = −2.04 days, 95% confidence interval = −2.57 to −1.52, I2 = 18%, and p = 0.00001); and hospital length of stay (mean differences = −2.9 days, 95% confidence interval = −4.24 to −1.56, I2 = 0%, and p = 0.00001).ConclusionThere is evidence that the use of nurse‐led weaning protocols for mechanically ventilated adult patients has a positive impact on weaning outcomes and patient safety.Relevance to clinical practiceThis review provides evidence supporting intensive care unit nurses' crucial role and abilities to lead weaning from mechanical ventilation.
MAL‐PDT inhibits oral precancerous cells and lesions via autophagic cell death
Authors: Wang Yen‐Yun , Chen Yuk‐Kwan , Hu Chu‐Sung , Xiao Ling‐Yi , Huang Wan‐Ling , Chi Tsung‐Chen , Cheng Kuang‐Hung , Wang Yun‐Ming , Yuan Shyng‐Shiou F. 
Published: January 2019

AbstractBackgroundOral cancer is a common cancer with a high mortality rate. While surgery is the most effective treatment for oral cancer, it frequently causes deformity and dysfunction in the orofacial region. In this study, methyl aminolevulinate photodynamic therapy (MAL‐PDT) as a prevention tool against progression of precancerous lesion to oral cancer was explored.MethodsFor in vitro studies, we evaluated the effects of MAL‐PDT on viability of DOK oral precancerous cells by XTT, cell morphology by TEM, and intracellular signaling pathways by flow cytometry, Western blotting, and immunofluorescence. For in vivo study, DMBA was used to induce oral precancerous lesions in hamsters followed by MAL‐PDT treatment. We measured tumor size and body weight weekly. After sacrifice, buccal pouch lesions were processed for H&E stain and immunohistochemistry analysis.ResultsMAL‐PDT induced autophagic cell death in DOK oral precancerous cells. The autophagy‐related markers LC3II and p62/SQSTM1 and autophagosome formation in DOK cells were increased after MAL‐PDT treatment. In vivo, Metvix®‐PDT treatment decreased tumor growth and enhanced LC3II expression in hamster buccal pouch tumors induced by DMBA.ConclusionsOur in vitro and in vivo results suggest that MAL‐PDT may provide an effective therapy for oral precancerous lesions through induction of autophagic cell death.