bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emerging trends and hotspots in intestinal microbiota research in sepsis: bibliometric analysis
Authors: Zhang Zhengyi , Yang Meijie , Zhou Tong , Chen Yingjie , Zhou Xiujuan , Long Kunlan 
Published: November 2024

BackgroundThe association between the gut microbiota and sepsis has garnered attention in the field of intestinal research in sepsis. This study utilizes bibliometric methods to visualize and analyze the literature on gut microbiota research in sepsis from 2011 to 2024, providing a scientific foundation for research directions and key issues in this domain.MethodsOriginal articles and reviews of gut microbiota research in sepsis, which published in English between 2011 and 2024, were obtained from the Web of Science Core Collection on June 21, 2024. Python, VOSviewer, and CiteSpace software were used for the visual analysis of the retrieved data.ResultsA total of 1,031 articles were analyzed, originating from 72 countries or regions, 1,614 research institutions, and 6,541 authors. The articles were published in 434 different journals, covering 89 different research fields. The number of publications and citations in this research area showed a significant growth trend from 2011 to 2024, with China, the United States, and the United Kingdom being the main research forces. Asada Leelahavanichkul from Thailand was identified as the most prolific author, making him the most authoritative expert in this field. “Nutrients” had the highest number of publications, while “Frontiers in Cellular and Infection Microbiology,” “Frontiers in Immunology” and “the International Journal of Molecular Sciences” have shown increasing attention to this field in the past 2 years. Author keywords appearing more than 100 times included “gut microbiota (GM),” “sepsis” and “microbiota.” Finally, this study identified “lipopolysaccharides (LPS),” “short-chain fatty acids (SCFAs),” “probiotics,” “fecal microbiota transplantation (FMT)” and “gut-liver axis” as the research hotspots and potential frontier directions in this field.ConclusionThis bibliometric study summarizes current important perspectives and offers comprehensive guidance between sepsis and intestinal microbiota, which may help researchers choose the most appropriate research directions.
Identification of IspD as a novel target for tuberculosis treatment using compound M6
Authors: Dong Lijun , Qi Hui , Zhu Yue , Yang Yuma , Zhao Yue , Zhang Sihan , Su Yongqiang , Yue Taiyun , Du Xiancai , Lei Hetian , Yang Yanhui 
Published: November 2024

IntroductionTuberculosis (TB) is a serious infectious disease that endangers human health, and TB becomes more difficult in eradiation due to its multidrug resistance (MDR). The objective of this research was to identify novel targets for treating TB.MethodsA 2-fold serial dilution method was used to determine minimal inhibitory concentrations (MIC) of compound M6 against Mycobacterium smegmatis (M. smegmatis). Compound M6 was subjected to reverse molecular docking with seven Mycobacterium tuberculosis proteins, and the best binding protein with the highest LibDock score was evaluated. The target protein with the highest score was purified through prokaryotic expression. Isolated target proteins were investigated for the enzyme activities and for the kinetic effect of compound M6 by absorbance detection. Subsequently, the CRISPR/Cas9 technology was employed to inhibit target gene expression for detecting MIC changes. Finally, potential targets were evaluated for the effect of the compound M6 in bacteria.ResultsThe MIC values of compound M6 against M. smegmatis were 32 μg/mL. The results from reverse molecular docking show that IspD has the highest LibDock score of 142.50, followed by Rv0674, IspF, and Dxr, with docking scores of 110.762, 71.6955, and 57.7446, respectively. IspD is a key enzyme in the 2-C-methyl-D-erythritol 4-phosphate pathway of MTB. The aKi and Ki values of M6 for the substrate MEP are 609.58 μM and 81.33 μM. For CTP, the aKi and Ki values are 657.89 μM and 40.07 μM. With tetracycline inducing CRISPR/Cas9 to suppress the expression of IspD, the MIC value of M6 against IspD went down significantly from 32 μg/mL to 4 μg/mL.ConclusionIspD is a novel target of the compound M6 for treating TB.
Nephrology intervention to avoid acute kidney injury in patients awaiting cardiac surgery: randomized clinical trial
Authors: Codina Sergi , Oliveras Laia , Ferreiro Eva , Rovira Aroa , Coloma Ana , Lloberas Nuria , Melilli Edoardo , Hueso Miguel , Sbraga Fabrizio , Boza Enric , Vazquez José M. , Pérez-Fernández José L. , Sabater Joan , Cruzado Josep M. , Montero Nuria 
Published: November 2024

IntroductionCardiac surgery-associated acute kidney injury (CSA-AKI) is a well-known complication that increases morbidity and mortality rates. The objective of this study was to reduce CSA-AKI through nephrologist intervention in patients awaiting cardiac surgery.MethodsWe performed a single center, open-label, randomized clinical trial including 380 patients who underwent scheduled cardiac surgery at the Hospital de Bellvitge between July 2015 and October 2019. A total of 184 patients were evaluated by the same Nephrologist one month before the surgery to minimize the risk factors for AKI. In addition to assessments at the outpatient clinic, we also collected clinical data during hospitalization and during the first year.ResultsDespite the intervention, no differences were observed between the groups in the incidence of CSA-AKI (intervention group 26.37% vs. standard of care 25.13%, p=0.874), mortality (3.91% vs. 3.59%, p=0.999), length of Intensive Care Unit (ICU) stay (10 days [7.00;15.0] for both groups, p=0.347), or renal function after one year of follow-up (estimated glomerular filtration rate (eGFR) by CKD-EPI: 74.5 ml/min (standard deviation 20.6) vs 76.7 (20.8) ml/min, respectively, p=0.364). A reduction in the need for blood transfusion was observed in the intervention group, although the difference was not statistically significant (37.22% vs. 45.03%, p =0.155).ConclusionIn this clinical trial, nephrologist intervention in the entire population on the cardiac surgery waiting list did not show a nephroprotective benefit.Clinical trial registrationClinicalTrials.gov, identifier (NCT02643745).
Hemodynamic assessment of intracranial atherosclerotic stenosis: comparison between invasive non-hyperemic pressure ratio and angiography-derived quantitative flow ratio
Authors: Wang Xiaohui , Bian Yang , Zhang Rongju , Zhu Haojing , Yang Junjie , Wang Ruiling , Liu Xinfeng , Lv Bin , Cao Xiangyu , Dai Wei , Sun Zhibin , Jing Jing , Du Zhihua , Yu Shengyuan , Wang Jun 
Published: November 2024

ObjectivesTo evaluate the diagnostic accuracy of the quantitative flow ratio (QFR) for hemodynamic exploration of intracranial atherosclerotic stenosis, using the invasive cerebrovascular pressure ratio (CVPR) and resting full-cycle ratio (RFR) as reference standards.Materials and methodsPatients with symptomatic unifocal intracranial atherosclerotic stenosis were included. The CVPR was defined as the ratio of the proximal and distal pressures. All patients underwent angioplasty under general anesthesia. The QFR was calculated based on digital subtraction angiography. Using the CVPR as a reference, we compared its correlation with the QFR across different degrees and locations of stenosis.ResultsThe CVPR and QFR were measured in 34 vessels of 32 patients. The QFR demonstrated a high correlation and excellent agreement(r = 0.8227, p &lt; 0.001) with the CVPR in distal stenosis before intervention. In the subgroup with diameter stenosis &gt;80%, the QFR showed a high correlation (r = 0.8812, p &lt; 0.001) with the CVPR. In the anterior circulation subgroup, the QFR showed an excellent correlation (r = 0.9066, p &lt; 0.001) with the CVPR. In the posterior circulation subgroup, the QFR showed a high correlation with the CVPR (r = 0.7706, p &lt; 0.001). Diameter stenosis rates showed a moderate negative correlation with the CVPR.ConclusionThere was a strong correlation between the QFR and wire-based CVPR, especially for anterior circulation lesions before intervention. The QFR may serve as a predictive factor for evaluating hemodynamic changes in intracranial atherosclerotic stenosis.
Biopsychosocial rehabilitation therapy in small fiber neuropathy: research protocol to study the effect of rehabilitation treatment
Authors: Damci Aysun , den Hollander Marlies , Hoeijmakers Janneke G. J. , Faber Catharina G. , Goossens Mariëlle E. J. B. , Verbunt Jeanine A. M. C. F. 
Published: November 2024

BackgroundSmall fiber neuropathy (SFN) is a chronic neuropathic pain condition that can lead to a decreased quality of life (QOL) and disability. Current pain treatment is mainly symptomatic, consisting of analgesics, with often disappointing results. There is a need for new, more effective treatment modality. Treatment based on a biopsychosocial approach on SFN-related pain may be a promising alternative. A rehabilitation treatment study protocol is presented with the following main objective: to test the effect of a tailored interdisciplinary rehabilitation treatment targeting both cognitive and psychological factors related to pain, in decreasing disability, and improving QOL in SFN.MethodsSingle-case experimental design. Ten participants with SFN will be included. Every patient will be offered a personalized program based on one of three rehabilitation treatment modules (graded activity, exposure in vivo or acceptance and commitment therapy) depending on the most prominent factor maintaining disability. Treatment will be provided for at least 8 weeks with 2 sessions a week.Discussion/conclusionThis is the first study investigating personalized rehabilitation treatment in patients with idiopathic SFN. The findings are expected to result in an effective treatment for SFN with an increase in QOL and a decrease in disability.Clinical trial registrationClinicalTrials.gov, identifier NCT05798949.
Beta-gamma phase-amplitude coupling of scalp electroencephalography during walking preparation in Parkinson’s disease differs depending on the freezing of gait
Authors: Kimoto Yuki , Tani Naoki , Emura Takuto , Matsuhashi Takahiro , Yamamoto Takuto , Fujita Yuya , Oshino Satoru , Hosomi Koichi , Khoo Hui Ming , Miura Shimpei , Fujinaga Takahiro , Yanagisawa Takufumi , Kishima Haruhiko 
Published: November 2024

IntroductionDespite using beta oscillations within the subthalamic nucleus as a biomarker of akinesia or rigidity in Parkinson’s disease, a specific biomarker for freezing of gait (FOG) remains unclear. Recently, scalp phase-amplitude coupling (PAC) measured through scalp electroencephalography (EEG) has emerged as a promising tool for analyzing brain function. In this study, we examined whether PAC could be a biomarker for FOG.MethodsWe enrolled 11 patients with Parkinson’s disease and recorded scalp EEG in preparation for and during gait while simultaneously assessing motor function, including FOG. We investigated changes in cortical PAC during walking with and without FOG and examined its correlation with the postural instability and gait difficulty (PIGD) score.ResultsPatient characteristics were as follows: mean age 59.1 ± 6.9 years, disease duration 13.9 ± 4.1 years, and seven men. Four trials were excluded from the analysis owing to artifacts. In the trials without FOG (n = 18), beta-gamma PAC in the sensorimotor area decreased during gait preparation (p = 0.011; linear mixed-effects model), which was not the case in trials with FOG (n = 6) (p = 0.64; linear mixed-effects model). Using a support vector machine, machine learning of PAC during preparation for walking predicted the presence of FOG with an accuracy of 71.2%. Conversely, PAC increased during walking in trials with FOG (p = 0.0042; linear mixed-effects model), and PAC 20 s after the start of walking was positively correlated with the PIGD score (correlation coefficient = 0.406, p = 0.032; Pearson’s rank correlation).ConclusionBeta-gamma PAC in the sensorimotor area during preparation for walking differs depending on the emergence of FOG. As gait symptoms worsened, beta-gamma PAC in the sensorimotor area during walking gradually increased. Cortical PAC may be a biomarker for FOG in Parkinson’s disease and may lead to the development of strategies to prevent falls in the future.
Effects of time-of-day resistance training on muscle strength, hormonal adaptations, and sleep quality during Ramadan fasting
Authors: Triki Raoua , Ben Abderrahman Abderraouf , Salhi Iyed , Rhibi Fatma , Saeidi Ayoub , Almaqhawi Abdullah , Hackney Anthony C. , Laher Ismail , Granacher Urs , Zouhal Hassane 
Published: November 2024

ObjectivesWe investigated the timing of resistance training (RT) during Ramadan fasting (RF) on muscle strength, hormonal adaptations, and sleep quality.MethodsForty healthy and physically active male Muslims (age = 25.7 ± 5.6 years, body mass = 85.1 ± 17.5 kg, height = 175 ± 9 cm, BMI = 28.3 ± 5.7 kg/m2) were enrolled in this study and 37 completed pre and post-tests. Subjects were randomly allocated into two experimental groups. Group 1 (FAST, n = 20) completed an 8-week whole-body RT in the late afternoon (between 16 h and 18 h) while fasting. Group 2 (FED, n = 20) completed the similar RT protocol compared with FAST at night (between 20 h and 22 h). The following parameters were analyzed at various time-points: 2 weeks before the start of RF (T0), on the 15th day of Ramadan (T1), on the 29th day of Ramadan (T2), and 21 days after the last day of RF (T3) where both groups were in a fed state. One-repetition maximum tests (1-RM) were conducted for the squats (1-RMSQ), the deadlift (1-RMDL) and the bench press (1-RMBP). Sleep quality was assessed using the full Pittsburgh Sleep Quality Index (PSQI). Blood samples were taken to determine cortisol, testosterone and IGF-1 levels. Additionally, acute hormonal responses were evaluated before (BF), immediately after (AF), and 30 min after a RT session (AF-30 min) at T0, T1, T2, and T3.ResultsSignificant group-by-time interactions were identified for 1-RMSQ (p = 0.001; effect size [ES] = 0.43) and 1-RMDL (p = 0.001; ES = 0.36). Post-hoc tests indicated significant 1-RMSQ (p = 0.03; ES = 0.12) and 1-RMDL (p = 0.04; ES = 0.21) improvements from T0-T2 for FED. Additionally, significant group-by-time interactions were observed for the chronic effects on cortisol (p = 0.03; ES = 0.27) and testosterone levels (p = 0.01; ES = 0.32). Post-hoc tests indicated significant increases of cortisol levels among FAST at T1 and T2 compared to T0 (p = 0.05; ES = 0.41, p = 0.03; ES =  0.34) and a significant increase in cortisol levels in FED at T1 (p = 0.05; ES = 0.29) and T2 (p = 0.04; ES = 0.25). However, the observed increase was lower compared to FAST. Post-hoc tests also indicated significant increases of testosterone only among FED at T2 (p = 0.04; ES = 0.31). A significant group-by-time interaction was found for the acute effect of exercise on cortisol level (p = 0.04; ES = 0.34). The cortisol level immediately after RT was higher in FAST only at T1 (p = 0.03; ES = 0.39) and T2 (p = 0.05; ES = 0.22) compared with T0. No significant group-by-time interactions were identified for sleep quality (p = 0.07; ES = 0.43).ConclusionMuslims can safely practice RT during RF. However, training in a fed state during Ramadan might be more effective than during fasted state for the enhancement of maximal strength with better hormonal responses observed.
Nomogram for predicting testicular yolk sac tumor in children based on age, alpha-fetoprotein, and ultrasonography
Authors: Yu Huan , Wang Hui , Huang Yichen , Hu Huiyong , Zhang Yue , Wu Min , Lyu Yiqing , Chen Yan , Zhou Lijun , Liang Yan , Sun Huizhen , Lin Xiaoling , Xie Hua , Chen Fang 
Published: November 2024

ObjectiveTo establish a predictive model for distinguishing testicular benign or yolk sac tumors in children.MethodsWe retrospectively analyzed data for 119 consecutive patients with unilateral testicular tumors treated at a single institution from June 2014 to July 2020. The patients were divided into the benign (n = 90) and yolk sac (n = 29) tumor groups based on the pathological diagnosis. We recorded patient age, serum markers [serum alpha-fetoprotein (AFP), human chorionic gonadotropin], and tumor ultrasonic findings (maximum diameter, ultrasonic echo, blood flow signal). Predictive factors were identified using descriptive statistical methods. A nomogram was established for preoperative prediction. An additional 46 patients were used as a validation cohort to verify the model.ResultsPatients with testicular yolk sac tumors were younger (median age: 14.0 vs. 34.0 months, P = 0.001) and had a higher incidence of elevated AFP levels (93.1% vs. 2.2%, P &lt; 0.001). Ultrasonography indicated that testicular yolk sac tumors tended to have larger maximum diameters (26.5 ± 11.3 vs. 16.6 ± 9.2 cm, P &lt; 0.001), a higher proportion of hypoechoic masses (44.8% vs. 8.9%, P &lt; 0.001), and a higher incidence of masses with strong blood flow signals (93.1% vs. 5.6%, P &lt; 0.001). A nomogram based on age, AFP levels, and ultrasound blood flow signals effectively predicted the probability of yolk sac tumor in children, with an accuracy of 0.98 (95% confidence interval: 0.984–1.003). The Brier score of the nomogram was 0.0002.ConclusionA nomogram based on age, AFP levels, and ultrasound blood flow signals can effectively predict the probability of testicular yolk sac tumor preoperatively, aiding in clinical decision-making and patient counseling.
Soil test crop response nutrient prescription equations for improving soil health and yield sustainability—a long-term study under Alfisols of southern India
Authors: Murthy R. Krishna , Nagaraju Bhavya , Govinda K. , Uday Kumar S. N. , Basavaraja P. K. , Saqeebulla H. M. , Gangamrutha G. V. , Srivastava Sanjay , Dey Pradip 
Published: November 2024

IntroductionEnhancing soil health and nutrient levels through fertilizers boosts agricultural productivity and global food security. However, careful fertilizer use is essential to prevent environmental damage and improve crop yields. The soil test crop response (STCR) is a scientific approach to fertilizer recommendation that ensures efficient use, supporting higher crop production while protecting the environment and preserving resources.MethodologyA long-term field experiment on the STCR approach was initiated in 2017 at the Zonal Agriculture Research Station, University of Agricultural Sciences, Bangalore, India. The experiment aimed to study the impact of STCR-based nutrient prescription along with farmyard manure (FYM) for a targeted yield of soybean (Glycine max), sunflower (Helianthus annuus), dry chili (Capsicum annuum), aerobic rice (Oryza sativa L.), foxtail millet (Setaria italica), okra (Abelmoschus esculentus), and kodo millet (Paspalum scrobiculatum) on yield and changes in soil health in comparison with other approaches of fertilizer recommendation.ResultsThe results showed a significant and positive impact of the integrated use of fertilizer with FYM based on the STCR approach on the productivity of all the crops and soil fertility. Significantly higher yields of soybean (23.91 q ha−1), sunflower (27.13 q ha−1), dry chili (16.67 q ha−1), aerobic rice (65.46 q ha−1), foxtail millet (14.07 q ha−1), okra (26.82 t ha−1), and kodo millet (17.10 q ha−1) were observed in the STCR NPK + FYM approach at yield level 1 compared to the general recommended dose and soil fertility rating approach. This approach outperformed the standard recommendations, enhancing nutrient uptake and efficiency across various crops. Utilizing the principal component analysis, the soil quality index effectively reflected the impact of nutrient management on soil properties, with the STCR NPK + FYM treatment at yield level 1 showing the highest correlation with improved soil physical and chemical parameters.DiscussionThe STCR approach led to improved yield, nutrient uptake, utilization efficiency, and soil health, thanks to a balanced fertilization strategy. This strategy was informed by soil tests and included factors like crop-induced nutrient depletion, baseline soil fertility, the efficiency of inherent and added nutrients through fertilizers and farmyard manure, and the success of yield-targeting techniques in meeting the nutritional needs of crops.
A large-scale screening identifies receptor-like kinases with common features in kinase domains that are potentially related to disease resistance in planta
Authors: Huang Yan , Yuan Yuan , Yang Rongqian , Gou Xiangjian , Dai Shiping , Zhou Jun , Guo Jinya , Shen Jinbo , Lu Yanli , Liu Yaxi , Cai Yi 
Published: November 2024

IntroductionThe plant genome encodes a plethora of proteins with structural similarity to animal receptor protein kinases, collectively known as receptor-like protein kinases (RLKs), which predominantly localize to the plasma membrane where they activate their kinase domains to convey extracellular signals to the interior of the cell, playing crucial roles in various signaling pathways. Despite the large number of members within the RLK family, to date, only a few have been identified as pattern-recognition receptors (PRRs), leaving many potential RLKs that could play roles in plant immunity undiscovered.MethodsIn this study, a recombinant strategy was initially employed to screen the kinase domains of 133 RLKs in the Arabidopsis genome to determine their involvement in the pathogen-triggered immunity (PTI) pathway. Subsequently, 6 potential immune-related recombinant RLKs (rRLKs) were selected for the creation of transgenic materials and underwent functional characterization analysis. Finally, a sequence analysis was conducted on the kinase domains of these 133 RLKs as well as the known immune RLK receptor kinase domains from other species.ResultsIt was found that 24 rRLKs activated the PTI response in Arabidopsis fls2 mutant protoplasts following flg22 treatment. Consistently, when 6 of these rRLKs were individually expressed in fls2 background, they exhibited diverse PTI signal transduction capabilities via different pathways while all retained membrane localization. Intriguingly, sequence analysis revealed multiple conserved amino acid sites within kinase domains of these experimentally identified immune-related RLKs in Arabidopsis. Importantly, these patterns are also preserved in RLKs involved in PTI in other species.DiscussionThis study, on one hand, identifies common features that theoretically can enhance our understanding of immune-related RLKs and facilitate the discovery of novel immune-related RLKs in the future. On the other hand, it provides experimental evidence for the use of recombinant technique to develop diverse rRLKs for molecular breeding, thereby conferring high resistance to plants without compromising their normal growth and development.
Adults’ and children’s reasoning about the potential of diverse groups
Authors: Weatherhead Drew , Nancekivell Shaylene E. , Workye Rebeka 
Published: November 2024

IntroductionIn two experiments, we examine the degree to which adults (Experiment 1) and children 5-to-8-years-old (Experiment 2) use diversity to infer a group’s cooperative and innovative potential.MethodsParticipants heard a child-friendly vignette about a competition in which a homogenous and diverse group were competing to design the perfect toy. They were then probed using questions related to the group’s innovative potential and cooperative potential and asked to justify their responses.ResultsResults show that adults believed that the diverse group would produce the more innovative product, while children endorsed the homogenous group. When asked a question probing cooperation, adults selected the homogenous group, whereas children were equally likely to select either group. Analysis of adults’ explanations showed their explanations differed depending on which group they endorsed. However, children’s explanations did not show this nuance. Exploratory analyses suggest that participants’ responses were influenced by their personal experiences with gender and racial diversity.DiscussionPeople’s appreciation of the link between group diversity and group potential changes across the lifespan likely due to life experiences.