bluuebunny/crossref_metadata_2025_split huggingface dataset:
Altered Factor VIII Complexes in Patients with Acute Respiratory Insufficiency
Authors: Grant K , Rodvien R , Mielke C H 
Published: July 2018

SummaryAcute respiratory failure is an often-fatal syndrome of multiple etiologies in which altered factor VIII may be a marker of endothelial disease. 12 women with overwhelming viral pneumonia were studied with serial factor VIII antigen, procoagulant activity, and von Willebrand’s factor assays. Antigen levels were elevated (range: 86-1644%) out of proportion to procoagulant activity (range: 35-521 % by a one-stage assay), and factor VIII antigen to activity ratios were as high as 16:1. Von Willebrand’s factor was normal but correlated best with procoagulant activity. All patients had abnormal antigen patterns on crossed immunoelectrophoresis, with increases in protein of both fast and slow mobility. These changes in factor VIII correlated with the patients’ clinical courses.
Heparin from Intestine and Lung Source Administered in Large Dose to Dogs with Acute Myocardial Ischemia
Authors: Saliba Michael J , Pavalec Richard J 
Published: July 2018

SummaryIntestinal mucosa heparin (IMH) and beef lung heparin (BLH) were infused into dogs subjected to myocardial ischemia by intermittent coronary artery occlusions. The IMH was from a mixture of beef, sheep, and pig intestinal mucosa. Initial control occlusion and recovery was followed by a second occlusion with 60,000 units of IMH or BLH added. Electrocardiographic S-T segment elevations (ST) were measured acutely. There were no significant differences in ST in non-ischemic myocardium before occlusions or with occlusions. In ischemic myocardium, IMH significantly lowered control ST 84% in amount (t = 6.1 p <0.00005), and 76% in number (t = 11.6 p <0.00001). BLH lowered control ST a significant, lesser, 36% in amount (t = 3.6 p <0.008), and 35% in number (t = 3.2 p <0.01). The difference between IMH and BLH in ischemic myocardium was a significant 48% in amount (t = 4.0 p <0.0007), and 41% in number (t = 2.0 p <0.06). Myocardial adenosine triphosphate (ATP) levels were assayed after 90 min. ATP levels were 31% higher in both ischemic and non-ischemic myocardium in IMH-treated dogs than in BLH- treated. It was concluded that IMH and BLH are functionally different, and IMH was significantly more effective.
Relationship of Factor IX Antigen and Coagulant in Hemophilia B Patients and Carriers
Authors: Pechet Liberto , Tiarks Cheryl Y , Stevens Joyce , Sudhindra Ramakrishna R , Lipworth Leslie 
Published: July 2018

SummaryA neutralizing and precipitating monospecific antiserum to human factor IX was developed. Plasmas from 224 hemophilia B patients, 39 obligatory and 38 possible carriers were studied by coagulant assays and Laurell’s electroimmunoassays. Based on factor IX coagulant (IX-C) to IX antigen (IX-Ag) ratios, the patients were classified as being either concordant (ratios ≥0.71) or discordant (<0.71). The diagnostic classification into concordant and discordant patterns is difficult in patients with low levels of antigen where the two forms may merge due to the insensitivity of the assay below 12%. Of the total cases studied by us, 36% were discordant (excess antigen). This is in agreement with 33.66%, a composite figure representing 199 cases described in five recently published papers, and indicates that approximately one-third of the hemophilia B population is of the discordant type. The pattern, when established, was found consistent in each kinship studied.Crossed antigen-antibody electrophoresis did not reveal any abnormalities in the hemophilia B plasmas with measurable IX-Ag.We found a low incidence of the BM variety. Determining this type of hemophilia B did not appear to increase the degree of predictability of the carrier state.Studies of carriers demonstrated that immunologic techniques did not contribute to the predictability of the carrier state in pedigrees of the concordant variety. In the discordant group a factor IX-C/IX-Ag ratio of 0.7 or less indicated that a female was very likely to be a carrier. An improvement in the prediction of the carrier state in females of the discordant pattern was demonstrated.
Factor VII Antigen Levels in a Healthy Blood Donor Population
Authors: Howard P R , Bovill E G , Pike J , Chruch W R , Tracy R P 
Published: July 2018

SummaryWe determined factor VII antigen (FVIIag) levels in 705 healthy blood donors ranging in age from 17 to 79 years using a two-site solid-phase enzyme immunoassay developed in our laboratory. The mean (± SD) FVIIag level for the total population was 102 ± 31%. FVIIag levels for men (n = 375) and women (n = 330) were 101 ± 28% and 103 ± 33%, respectively. A significant increase in FVIIag was observed with age in both men (r = 0.25, p <0.0001) and women (r = 0.35, p <0.0001). FVIIag levels were significantly higher in women > 60 years when compared to men (median women: 125%; median men: 111%; p <0.05). On a subset of the study group (n = 45), FVIIag levels were correlated to total cholesterol (r = 0.27, p = 0.08) and triglyceride (r = 0.41, p <0.01). Assuming the commonly used reference interval of 60-140% for FVII, the frequencies of FVIIag values for <60% and >140% using our assay were 2.1% and 9.2%, respectively. Gender and age-related differences in FVIIag levels must be considered in a reference interval. We further suggest that assay-specific reference ranges be established, which may include values outside the commonly used values of 60-140%.
Increased Mononuclear Cell Tissue Factor and Type-2 Plasminogen Activator Inhibitor and Reduced Plasma Fibrinolytic Capacity in Children with Lymphoma
Authors: Semeraro N , Montemurro P , Giordano P , Santoro N , De Mattia D , Colucci M 
Published: July 2018

SummaryBlood clotting activation and fibrin deposition are common findings in lymphoma patients. We evaluated the capacity of peripheral blood mononuclear cells to produce procoagulant activity (PCA) and plasminogen activator inhibitor (PAI) in 12 children with newly diagnosed lymphoma (8 non-Hodgkin’s, 4 Hodgkin’s) and in 12 matched healthy donors. In the same subjects we also measured plasma antigen levels of tissue-type PA (t-PA), urokinase-type PA (u-PA), PAI-1, PAI-2, and D-dimer. PCA generated by mononuclear cells after incubation for 20 h at 37° C was significantly higher in patients than in controls (p = 0.027). In all samples it was identified as tissue factor by functional criteria (dependence on factor VII). Moreover, culture medium obtained from patients’ mononuclear cells after incubation for 20 h at 37° C contained significantly higher amounts of PAI activity and PAI-2 antigen than control samples (p <0.001). Plasma PAI-1 and t-PA antigens were significantly augmented in patients (p <0.005), the mean increase of PAI-I being about 5 times higher than that of t-PA. Plasma levels of D-dimer wete markedly increased in the patients’ group (p <0.001), whereas u-PA and PAI-2 antigens did not differ from controls. It is suggested that monocytes from lymphoma patients are endowed with functional abnormalities leading to the simultaneous expression of tissue factor and antifibrinolytic activity. These abnormalities, coupled with a reduced plasma fibrinolytic potential, could play an important pathogenetic role in blood clotting activation and fibrin deposition associated with lymphoma.
Six Novel and Three Recurrent Mutations in Nine Austrian Patients with Hemophilia B
Authors: Walter J , Pabinger-Fasching I , Watzke H H 
Published: July 2018

SummaryIn this report we describe the molecular basis of the factor IX (FIX) deficiency in nine patients with severe (n = 6), moderate (n = 1) or mild (n = 2) hemophilia B. The following genetic defects were identified by enzymatic amplification with the polymerase chain reaction (PCR) and subsequent direct sequencing of all exons and exon-intron-junctions: patient B.B. (FIX “Vienna I”): deletion of nucleotides 6343 to 6362; patient M.H. and W. J. (FIX “Vienna II”): nucleotide 17704 (C to G), Gin 97 to Glu; patient L. K. (FIX “Vienna III”): nucleotide 17761 (C to T), Arg 116 to stop; patient U. A. (FIX “Vienna IV”): nucleotide 10415 (C to G), Pro 55 to Ala; patient H.G. (FIX “Vienna V”): nucleotide 6488 (C to T), Thr 38 to lie; patient H. M. (FIX “Vienna VI”): nucleotide 31276 (G to C), Trp 385 to Cys; patient L. C. (FIX “Vienna VII”): deletion of nucleotide 6700; patient S.F. (FIX “Vienna VIII”): nucleotide 10392 (A to T), Asp 47 to Val. The causative mutation was detected in the FIX gene in each of the nine patients with hemophilia B. There was one small deletion, one point deletion and seven point mutations. The latter include six missense mutations and one nonsense mutation. The mutations in Vienna III, IV and V have already been described in previous studies. The two deletions, Vienna I and Vienna VII have not been reported previously. The genetic defects observed in Vienna II, VI and VIII are novel missense mutations which result in amino acid changes at residues 97,47 and 385, respectively.
D-Dimer Determination to Exclude Pulmonary Embolism: a Two-Step Approach Using Latex Assay as a Screening Tool
Authors: de Moerloose P , Minazio Ph , Reber G , Perrier A , Bounameaux H 
Published: July 2018

SummaryD-dimer (DD), when measured by a quantitative enzyme-linked immunosorbent assay (ELISA), is a valuable test to exclude venous thromboembolism (VTE). However, DD ELISA technique is not appropriate for emergency use and the available agglutination latex assays are not sensitive enough to be used as an alternative to rule out the diagnosis of VTE. Latex assays could still be used as screening tests. We tested this hypothesis by comparing DD levels measured by ELISA and latex assays in 334 patients suspected of pulmonary embolism. All but one patient with a positive (DD ≥500 ng/ml) latex assay had DD levels higher than 500 ng/ml with the ELISA assay. Accordingly, ELISA technique could be restricted to patients with a negative result in latex assay. This two-step approach would have spared about 50% of ELISA in our cohort. In conclusion, our data indicate that a latex test can be used as a first diagnostic step to rule out pulmonary embolism provided a negative result is confirmed by ELISA and the performance of the latex assay used has been assessed properly.
Circulating Endothelial Cell/Leukocyte Adhesion Molecules in Atherosclerosis
Authors: Blann Andrew D , McCollum Charles N 
Published: July 2018

SummarySoluble adhesion molecules E-selectin, intercellular adhesion molecule (sICAM) and vascular cell adhesion molecule (sVCAM) were measured alongside von Willebrand factor (vWf) in 40 patients with peripheral vascular disease (PVD), 43 with ischaemic heart disease (IHD) and in equal numbers of age and sex matched asymptomatic controls. Increased vWf was found in patients with IHD (p = 0.0008) and in patients with PVD (p = 0.0001) relative to their respective controls but levels did not differ between the two patient groups. Raised sICAM was found in both PVD (p = 0.0003) and IHD (p = 0.0059) compared to their respective controls and was higher in PVD than in IHD (p = 0.0088). In the subjects taken as a whole, there was no correlation between vWf and sICAM. Levels of soluble E-sel-ectin and sVCAM did not differ in patients or controls. These data suggest that soluble ICAM may be useful as an index of endothelial cell activation in clinical manifestations of atherosclerosis.
Meningioma of the Pineal Region — Differential Radiologic Aspects of Pineal Region Tumors Based on a Clinical Case
Authors: Avelar Tiago , Paiva Aline , Costa Márcio , Aguiar Guilherme , Vitorino João , Veiga José 
Published: July 2018

AbstractPineal region tumors are uncommon among neoplasm of the central nervous system, with this region being the most heterogeneous in terms of histological types. Meningiomas are rarer still, but can be found at this site, with origins in either the velum interpositum or falcotentorial junction. Neuroimaging exams can distinguish malignant from benign lesions besides helping to define the origin of the lesion as the pineal parenchymal or surrounding structures. We report the case of a woman with a pineal region tumor in which differential diagnoses included meningioma and germinoma, with confirmation of the former based on radiological characteristics and histopathology. In addition, a brief review of differential diagnoses and approaches for cases of lesions in this region is provided.
Antifibrinolytic Activity of Certain Pectins
Authors: Nilsson Inga Marie , Björkman Sven Erik , Studnitz Wilfried v. , Hallén Acke 
Published: July 2018

SummaryIngestion of wine and beer, but not of ethyl alcohol, produced a decrease of the blood fibrinolytic activity. In vitro experiments showed that wine and beer contained a principle which inhibited fibrinolytic activators. This principle was isolated from grape pulp, and upon analysis it was found to be a pectin-like substance.
The Formation of Intermediate Product I in a Purified System The Role of Factor IX or of its Precursor and of a Hageman Factor-PTA Fraction
Authors: Yin E. T , Duckert F 
Published: July 2018

Summary1. The role of two clot promoting fractions isolated from either plasma or serum is studied in a purified system for the generation of intermediate product I in which the serum is replaced by factor X and the investigated fractions.2. Optimal generation of intermediate product I is possible in the purified system utilizing fractions devoid of factor IX one-stage activity. Prothrombin and thrombin are not necessary in this system.3. The fraction containing factor IX or its precursor, no measurable activity by the one-stage assay method, controls the yield of intermediate product I. No similar fraction can be isolated from haemophilia B plasma or serum.4. The Hageman factor — PTA fraction shortens the lag phase of intermediate product I formation and has no influence on the yield. This fraction can also be prepared from haemophilia B plasma or serum.
The Relation between a Naturally Occuring Inhibitor and Prothrombin in Intermediate Product I Formation
Authors: Yin E. T , Shanberge J. N , Duckert F 
Published: July 2018

Summary1. A method for the separation from clotting factors and the concentration of an inhibitor of intermediate product I present in adsorbed plasma and adsorbed serum is described.2. This inhibitor is not only stable but also activated at 37° C. At 4° C it loses activity.3. The inhibitory effect on the formation of intermediate product I is counteracted by small amounts of prothrombin or thrombin.4. After addition of inhibitor prothrombin is necessary for the formation of intermediate product I in the purified system.5. The discrepancy, in regard to the need for prothrombin (or thrombin) between the purified and the non-purified system can be explained by the relative amounts of inhibitor in the two systems.
The Prephase Accelerator. Present Status
Authors: Duckert F 
Published: July 2018

SummaryThe properties of the prephase accelerator (PPA) are indicated as well as its differentiation from other known clotting factors. PPA is either a reaction product or a degratation product. For its normal formation genuine factor IX, PTA and ? Hageman factor are necessary.The one-stage quantitative assay method for factor “IX” does not determine the factor lacking in haemophilia B. This method gives a measure of PPA.Some practical observations are made concerning the value of the one-stage assay method for factor “IX” and of the thromboplastin generation test for the diagnosis of haemophilia B.
Some Observations on the Rôle of Hageman Factor in Blood Coagulation
Authors: Haanen C , Hommes F , Morselt Gerdy 
Published: July 2018

SummaryThe defective thromboplastin formation in Hageman factor deficient plasma is completely corrected using incubation tubes which have been coated previously with normal plasma. Positively or negatively charged molecules as protamin and sulphated polysaccharides interfere with this correction in a way that gives evidence Hageman factor is a positively charged protein.The defective thromboplastin generation of Hageman factor deficient incubation mixtures is corrected by addition of a purified Hageman factor preparation. Pre-incubation of Hageman factor with HF deficient serum improves the thromboplastin formation further, while pre-incubation of Hageman factor with HF deficient plasma abolishes this correction complete. Possibly here lies the key for the fact that serum clotting factors augment and plasma factors deteriorate during the clotting process.
The Use of DEAE Cellulose in the Preparation of a Factor VIII (AHG)-free Bovine Fibrinogen Reagent
Authors: Stapp W. F 
Published: July 2018

SummaryAdequate fibrinogen reactivity is suggested as an additional requirement for specific fibrinogen reagents, to be added to the well known requirements of protein concentration, optimum pH, and optimum ionic strength. Reasons and a method for testing are outlined.A method for the preparation of a specific fibrinogen reagent, deficient in factor VIII, from BaSO4 adsorbed bovine plasma is described in detail. The method described utilizes either fresh or regenerated DEAE cellulose as an adsorbent in a non-column chromatographic adsorption procedure, associated with a dialysis procedure in which the final fibrinogen product is concentrated and precipitated as a “euglobulin”. The “euglobulin” fibrinogen precipitate was then dissolved/suspended in buffered saline at a specific pH and NaCl concentration. Essentially the method can be briefly outlined into 5 separate steps:1. Dialysis of BaSO4 adsorbed bovine plasma against 0.5% NaCl at pH 6.6 in 0.005 M Imidazole-HCl buffer.2. Washing of DEAE cellulose with the same type of buffered NaCl solution to specific conditions.3. Mixing of the dialyzed BaSO4 adsorbed bovine plasma with the washed DEAE cellulose and separation by centrifugation.4. Concentration of the cellulose adsorbed plasma as a “euglobulin” fibrinogen precipitate by dialysis against running tap water.5. Dissolving of the “euglobulin” fibrinogen precipitate in 0.9% NaCl buffered at pH 6.8.Fibrinogen preparations, prepared with the use of regenerated DEAE cellulose were found to be similar to those prepared by fresh DEAE cellulose in fibrinogen reactivity and deficiency in factor VIII activity, but were found to have less fibrinogen concentration than those prepared using fresh DEAE cellulose.Suggestions were also made for possibly increasing the over-all yield of the fibrinogen reagent through use of a “washing-elution” procedure.The procedure that was used for regenerating the DEAE cellulose was also described in detail and commented upon.
The Investigation of a Patient with an Unusual Coagulation Defect
Authors: Gomes Emiliano L. , Calicchio Therezinha R. , Biggs Rosemary 
Published: July 2018

Summary1. The coagulation defect of a patient whose blood contains an inhibitor is described.2. The inhibitor lengthens the clotting time of plasma in the presence of weak thromboplastin and also interferes with the mechanism of glass contact activation.3. It is suggested that the inhibitor is similar to those which occur in the blood of patients with disseminated lupus erythematosus.
On the Physiological Antiacogulating System
Authors: Kudrjashov B. A 
Published: July 2018

Summary1. The influence of intravenous injections of thrombin has been tested on frogs and rats.2. After thrombin or thromboplastin injection the animals produce anticoagulant activity which is, to a certain extent, quantitatively related to the activity injected.3. If the animals tested are under anaesthesia or if the medulla oblongata is switched off the humoral reflex reaction is blockaded.4. The importance of these observations for the prophylaxis of early postoperative thromboses is discussed.5. These results combined with the effect of thrombin injection on the clotting factors in vivo are used for the elaboration of a coagulation scheme.
The Influence of Fibrin Stabilizing Factor on the Growth of Fibroblats in vitro and Wound Healing
Authors: Beck E , Duckert F , Ernst M 
Published: July 2018

SummaryCongenital deficiency of Fibrin Stabilizing Factor (FSF) is the cause of both pathological haemostasis and poor wound healing. Experiments with fibroblast cultures were carried out to characterize the latter. Growth of the cultures in the patient’s plasma was quantitatively and qualitatively inferior as compared with the growth in normal control plasma, which contained FSF. Only by addition of normal plasma and purified FSF the poor cell growth was corrected. The necessity of fibrin present in the first stage of wound healing and possible modes of action of FSF in haemostasis and wound healing are discussed.
A Modified Thromboplastin Screening Test
Authors: Macpherson Janet C. , Hardisty R. M 
Published: July 2018

SummaryA modification of the thromboplastin screening test of Hicks and Pitney is described, in which the effect of surface contact on the test plasma is controlled by the addition of a suspension of kaolin to the incubation mixture before recalcification.Comparative studies show the modified test to give more reproducible results than the standard method.
On the Behaviour of a Human Serum Proactivator Reacting with Streptokinase during Artificial Activation of the Fibrinolytic System
Authors: Doleschel W , Auerswald W 
Published: July 2018

SummaryDuring “spontaneous” activation of a human euglobulin preparation in suitably spaced samples — while plasminogen became progressively converted into piasmin — the proactivator content was tested by addition of equal amounts of streptokinase and evaluation of the lytic activities on heated and normal bovine fibrin plates. Indepedently of the decreasing content of plasminogen the proactivator which could be activated by streptokinase showed no significant change of concentration. These observation indicate that plasminogen is not acting as proactivator and that there exists a separate proactivator-activator system of the fibrinolytic mechanism in human serum.
Some Effects of Metal Cations on Blood Coagulation
Authors: Vulpis Nicoletta , Giorgino Riccardo 
Published: July 2018

SummaryThe action of some metal cations on blood coagulation was studied by means of t.e. graphic tracings. They exerted a delaying effect on coagulation process, as shown by the r increase and ma decrease. It is probably due to the formation of complex and reversible compounds. Adding a strong chelating agent, EDTA, caused perceptible reversal of the cation activity.
Tooth Extractions in Hemophilia Control of Bleeding without Use of Blood, Plasma or Plasma Fractions
Authors: Lucas O. N , Carroll R.T , Finkelmann A , Tocantins L.M 
Published: July 2018

SummaryHypnotic suggestion, loose packing of sockets and protection of the extractions area when properly done make it possible to carry out virtually painless tooth extractions in even the most severe hemophilics, with a minimum of hemorrhage and of physical or psychic trauma.Following the principles outlined 91 teeth were extracted from 20 hemophilic patients, 17 of whom had classical hemophilia “A” ranging in severity from mild to very severe (75 extractions), while 3 had hemophilia “B” (16 extractions). Fifty extractions were carried out without hospitalization.None of the extractions required pre or postoperative transfusions of blood, plasma or plasma fractions and there was no abnormal postoperative bleeding in any of the patients.A successful non-hemorrhagic extraction depends on effective cooperation between Oral Surgeon and Haematologist and the scrupulous execution of all three principles stated and not any single one in particular.