bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silencing of adaptor protein SH3BP2 reduces KIT/PDGFRA receptors expression and impairs gastrointestinal stromal tumors growth
Authors: Serrano‐Candelas Eva , Ainsua‐Enrich Erola , Navinés‐Ferrer Arnau , Rodrigues Paulo , García‐Valverde Alfonso , Bazzocco Sarah , Macaya Irati , Arribas Joaquín , Serrano César , Sayós Joan , Arango Diego , Martin Margarita 
Published: June 2018

Gastrointestinal stromal tumors (GISTs) represent about 80% of the mesenchymal neoplasms of the gastrointestinal tract. Most GISTs contain oncogenic KIT (85%) or PDGFRA (5%) receptors. The kinase inhibitor imatinib mesylate is the preferential treatment for these tumors; however, the development of drug resistance has highlighted the need for novel therapeutic strategies. Recently, we reported that the adaptor molecule SH3 Binding Protein 2 (SH3BP2) regulates KIT expression and signaling in human mast cells. Our current study shows that SH3BP2 is expressed in primary tumors and cell lines from GIST patients and that SH3BP2 silencing leads to a downregulation of oncogenic KIT and PDGFRA expression and an increase in apoptosis in imatinib‐sensitive and imatinib‐resistant GIST cells. The microphthalmia‐associated transcription factor (MITF), involved in KIT expression in mast cells and melanocytes, is expressed in GISTs. Interestingly, MITF is reduced after SH3BP2 silencing. Importantly, reconstitution of both SH3BP2 and MITF restores cell viability. Furthermore, SH3BP2 silencing significantly reduces cell migration and tumor growth of imatinib‐sensitive and imatinib‐resistant cells in vivo. Altogether, SH3BP2 regulates KIT and PDGFRA expression and cell viability, indicating a role as a potential target in imatinib‐sensitive and imatinib‐resistant GISTs.
Synthesis and Evaluation of a Magnetic Molecularly Imprinted Polymer Sorbent for Determination of Trace Trichlorfon Residue in Vegetables by Capillary Electrophoresis
Authors: Li Deqing , Qiao Xuguang , Lu Juxiu , Xu Zhixiang 
Published: June 2018

AbstractIn this study, a magnetic molecularly imprinted polymer (MMIP) based on Fe3O4@SiO2@CdTe (CdTe@MMIP) was synthesized using trichlorfon as template molecule, methacrylic acid (MAA) as the functional monomer, and ethylene glycol dimethacrylate (EGDMA) as the cross‐linker. The prepared MMIP was characterized, and the results showed that it exhibited good recognition and selective ability, offered a faster kinetics for the adsorption of trichlorfon. Scatchard analysis indicated that the binding sites in the CdTe@MMIP had two distinct groups (the high and low affinity binding sites). The saturated adsorption capacity (Qmax1) was 63.2 mg/g due to the low affinity binding sites, and another saturated adsorption capacity (Qmax2) was 71.4 mg/g due to the high affinity binding sites. Using it as sorbent, a method of molecularly imprinted magnetic solid‐phase extraction coupled with capillary electrophoresis (MIMSPE‐CE) for selective extraction, magnetic separation, and fast determination of trace trichlorfon in vegetable samples was developed. Under optimal conditions, appreciable sensitivity was achieved with a LOD of 30 μg/kg (S/N = 3) for the developed method. The relative standard deviation (RSD) for five replicate extractions of 0.05 mg/L trichlorfon standard solution was 4.2%. To evaluate the accuracy of this method, the blank cucumber and cauliflower samples spiked with trichlorfon were extracted and analyzed by this method with good recoveries ranging from 78.7% to 96.6%. Moreover, this method was applied to the quantitative detection of the trichlorfon residues in rape samples, and the results correlated well with that obtained from the gas chromatography method.
Control over Self‐Doping in High Band Gap Perovskite Films
Authors: Kulbak Michael , Levine Igal , Barak‐Kulbak Einav , Gupta Satyajit , Zohar Arava , Balberg Isaac , Hodes Gary , Cahen David 
Published: June 2018

AbstractIt is reported how differences in the composition of high bandgap Pb bromide‐based perovskites affect their carrier diffusion length and junction type. Pb‐based, APbX3, halide perovskite (HaP) films and devices are studied, where A can be a mixture of formamidinium, methylammonium (MA), and Cs, and X a mixture of Br and Cl, using a combination of dark‐ and photoconductivity and steady‐state photocarrier grating. The results show the cation and anion compositions affect both majority and minority carrier diffusion lengths. In particular, using electron beam‐induced current measurements, FTO\dTiO2mp‐TiO2HaP\PTAA (poly‐triarylamine)\Au devices are studied. The results enable identifying junction and built‐in voltage formation and track position and size of the space charge region width with changes in the HaP composition. As far as it is known, it is found for the first time that a mixed‐cation HaP forms a junction that has characteristics of a p‐i‐n one, with relatively long and comparable carrier diffusion lengths, while the single cation‐based bromide HaPs form clear p‐n junctions at the interface with the TiO2 [pure CsPbBr3 and MAPbBr3(Cl)] or a buried one (MAPbBr3) and shorter diffusion lengths. These differences are attributed to lower carrier density in MAPbBr3, and especially in the mixed cation HaP, which is comparable to iodide‐based HaP films.
Single vs multiple fraction palliative radiotherapy for uncomplicated painful bone metastases treated at University of Malaya Medical Centre: A single institutional Malaysian experience
Authors: Duraisamy Ina Shaliny , Saad Marniza , Alip Adlinda 
Published: June 2018

AbstractIntroductionThis study was conducted to compare pain response between single and multiple fraction palliative radiotherapy and to describe prognostic factors affecting treatment response in University of Malaya Medical Centre (UMMC).MethodsThe case records of 162 patients with uncomplicated painful bone metastases treated with palliative radiotherapy from 2006 to 2014 were analyzed. Treatment outcomes were pain score response, analgesic score response, response according to International Consensus Endpoints (complete response and overall response) at 4, 12, and 24 weeks, retreatment rate, symptomatic skeletal events (SSEs), and prognostic factors.ResultsAt 24 weeks, pain score response for single and multiple fraction group was 82.3% and 88.5%, analgesic score response was 54.8% and 61.5%, and overall response according to International Consensus Endpoint was 61.3% and 67.7%, respectively. There was no statistically significant difference in treatment response between the 2 treatment groups for all endpoints. ECOG (<2 vs ≥2: aOR 3.405, 95% CI 1.708‐6.790, P = .001) and primary breast and prostate (breast vs others: aOR 5.231, 95% CI 1.973‐13.869, P = .001; prostate vs others: aOR 5.522, 95% CI 1.493‐20.420, P = .01) were significant variables on multivariate analysis.ConclusionSingle fraction radiotherapy is as effective as multiple fraction radiotherapy for the palliation of uncomplicated bone metastases.
Fossil moonseeds from the Paleogene of West Gondwana (Patagonia, Argentina)
Authors: Jud Nathan A. , Iglesias Ari , Wilf Peter , Gandolfo Maria A. 
Published: June 2018

Premise of the StudyThe fossil record is critical for testing biogeographic hypotheses. Menispermaceae (moonseeds) are a widespread family with a rich fossil record and alternative hypotheses related to their origin and diversification. The family is well‐represented in Cenozoic deposits of the northern hemisphere, but the record in the southern hemisphere is sparse. Filling in the southern record of moonseeds will improve our ability to evaluate alternative biogeographic hypotheses.MethodsFossils were collected from the Salamanca (early Paleocene, Danian) and the Huitrera (early Eocene, Ypresian) formations in Chubut Province, Argentina. We photographed them using light microscopy, epifluorescence, and scanning electron microscopy and compared the fossils with similar extant and fossil Menispermaceae using herbarium specimens and published literature.Key ResultsWe describe fossil leaves and endocarps attributed to Menispermaceae from Argentinean Patagonia. The leaves are identified to the family, and the endocarps are further identified to the tribe Cissampelideae. The Salamancan endocarp is assigned to the extant genus Stephania. These fossils significantly expand the known range of Menispermaceae in South America, and they include the oldest (ca. 64 Ma) unequivocal evidence of the family worldwide.ConclusionsOur findings highlight the importance of West Gondwana in the evolution of Menispermaceae during the Paleogene. Currently, the fossil record does not discern between a Laurasian or Gondwanan origin; however, it does demonstrate that Menispermaceae grew well outside the tropics by the early Paleocene. The endocarps’ affinity with Cissampelideae suggests that diversification of the family was well underway by the earliest Paleocene.
Efficacy of chemotherapy or chemo‐anti‐PD‐1 combination after failed anti‐PD‐1 therapy for relapsed and refractory hodgkin lymphoma: A series from lysa centers
Authors: Rossi Cédric , Gilhodes Julia , Maerevoet Marie , Herbaux Charles , Morschhauser Franck , Brice Pauline , Garciaz Sylvain , Borel Cécile , Ysebaert Loïc , Obéric Lucie , Lazarovici Julien , Deau Bénédicte , Dupuis Jehan , Chauchet Adrien , Abraham Julie , Bijou Fontanet , Stamatoullas‐Bastard Aspasia , Malfuson Jean‐Valère , Golfier Camille , Laurent Camille , Pericart Sarah , Traverse‐Glehen Alexandra , Kanoun Salim , Filleron Thomas , Casasnovas René‐Olivier , Ghesquières Hervé 
Published: June 2018

AbstractAnti‐PD‐1 therapy provides high response rates in Hodgkin lymphoma (HL) patients who have relapsed or are refractory (R/R) to autologous stem cell transplantation (ASCT) and brentuximab vedotin (BV), but median progression free survival (PFS) is only one year. The efficacy of treatment following anti‐PD‐1 is not well known. We retrospectively investigated the efficacy of salvage therapies for unsatisfactory response to anti‐PD‐1 therapy, assessed by PET‐CT according to the Lugano criteria, in 30 R/R HL patients. Patients were highly pretreated before anti‐PD‐1 (70% received ASCT and 93% BV). Unsatisfactory responses to anti‐PD1 therapy were progressive disease (PD) (n = 24) and partial response (PR) (n = 6). For the 24 PD patients, median anti‐PD‐1 related PFS was 7.5 months (95%CI, 5.7‐11.6); 17 received subsequent CT alone (Group 1) and 7 received CT in addition to anti‐PD‐1 (Group 2). 16/24 patients (67%) obtained an objective response. In the 15 patients treated with the same CT, twelve obtained PR or complete response (CR). In Group 1, there were 7 CR (41%), 3 PR (18%), and 7 PD (41%). In Group 2, there were 4 CR (57%), 2 PR (29%), and 1 SD (14%). No unexpected toxicity was observed. Six patients who achieved response proceeded to allogeneic SCT. With a median follow‐up of 12.1 months (7‐14.7), the median PFS following the initiation of CT was 11 months (95% CI 6.3‐not reached) and the median of overall survival was not reached. These observations in highly pretreated HL patients suggest that anti‐PD‐1 therapy might re‐sensitize tumor cells to CT.
Combined effect of attrition and ultrasound on the disruption of Pseudomonas putida for the efficient release of arginine deiminase
Authors: Patil Mahesh D. , Shinde Ashok S. , Dev Manoj J. , Patel Gopal , Bhilare Kiran D. , Banerjee Uttam Chand 
Published: June 2018

Disruption of Pseudomonas putida KT2440 by ultrasound treatment in a bath sonicator, in presence of the glass beads, was carried out for the release of arginine deiminase (ADI) and the results were compared with that of by Dyno‐mill. The release of ADI depended mainly on the bead size and cellmass concentration being disrupted in bead mill. Nearly 23 U mL−1 ADI was released when slurry with a cell‐mass concentration of 250 g L−1 was disintegrated for 9 min with 80% bead loading (0.25 mm) in Dyno‐mill. Marginally higher amount of ADI (24.1 U mL−1) was released by the bath sonication of 250 g L−1 cellmass slurry for 30 min with the beads (0.1 mm) and a sonication power of 170 W. The glass beads, suspended along with the cellmass slurry in bath sonicator, efficiently disrupted the microbial cells to release ADI. Variation in the kinetic constants for the performance parameters implied that ADI release and cell disruption kinetics is a function of disruption technique used and the process variables thereof. Estimation of location factor suggested that selective release of ADI can be achieved. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018 © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1185–1194, 2018
Tyrosine kinase inhibitor‐induced IL‐6/STAT3 activation decreases sensitivity of EGFR‐mutant non‐small cell lung cancer to icotinib
Authors: Wang Jinyao , Wang Yizhe , Zheng Chunlei , Hou Kezuo , Zhang Tieqiong , Qu Xiujuan , Liu Yunpeng , Kang Jian , Hu Xuejun , Che Xiaofang 
Published: June 2018

AbstractTyrosine kinase Inhibitors (TKIs) of epidermal growth factor receptor (EGFR) has considerably benefited for non‐small cell lung carcinomas (NSCLC) harbor mutations in EGFR. However, the factors attenuating EGFR‐TKI efficiency are obstacles to inhibit the proliferation of EGFR‐mutant NSCLC cells successfully. Clarifying the insensitivity mechanisms of EGFR‐TKI would help to develop new treatment strategy. In this study, the sensitivity of EGFR‐mutant NSCLC cell lines, PC9 and HCC827, to icotinib was detected. Similar with other EGFR‐TKIs such as gefitinib and erlortinib in previous research, the proliferation of two cell lines was apparently inhibited. However, we surprisingly found that contrast with the suppression of EGFR‐AKT/ERK pathway, STAT3 was significantly activated in PC9 cells with the treatment of icotinib, but not in HCC827 cells. Further study confirmed that icotinib concomitantly induced IL‐6 secretion and src activation in PC9 cells. Moreover, with the treatment of IL‐6 neutralizing antibody or src inhibitor, dasatinib, icotinib‐induced phosphorylation of STAT3 was reduced, as well as the sensitivity of PC9 to icotinib was also partially increased. Our results suggest that Src/IL‐6/STAT3 bypass pathway is activated to maintain cell survival when the EGFR pathway was inhibited by TKIs, even in some EGFR‐mutant NSCLC cells sensitive to TKIs. This finding provides a groundwork for potential combinatorial treatment with TKIs and Src or STAT3 inhibitor to improve icotinib sensitivity.
Genome‐wide identification, phylogenetic classification, and exon–intron structure characterization of the tubulin and actin genes in flax (Linum usitatissimum)
Authors: Pydiura Nikolay , Pirko Yaroslav , Galinousky Dmitry , Postovoitova Anastasiia , Yemets Alla , Kilchevsky Aleksandr , Blume Yaroslav 
Published: June 2018

AbstractFlax (Linum usitatissimum L.) is a valuable food and fiber crop cultivated for its quality fiber and seed oil. α‐, β‐, γ‐tubulins and actins are the main structural proteins of the cytoskeleton. α‐ and γ‐tubulin and actin genes have not been characterized yet in the flax genome. In this study, we have identified 6 α‐tubulin genes, 13 β‐tubulin genes, 2 γ‐tubulin genes, and 15 actin genes in the flax genome and analyzed the phylogenetic relationships between flax and Arabidopsis thaliana tubulin and actin genes. Six α‐tubulin genes are represented by three paralogous pairs, among 13 β‐tubulin genes 7 different isotypes can be distinguished, 6 of which are encoded by two paralogous genes each. γ‐tubulin is represented by a paralogous pair of genes one of which may be not functional. Fifteen actin genes represent seven paralogous pairs—seven actin isotypes and a sequentially duplicated copy of one of the genes of one of the isotypes. Exon–intron structure analysis has shown intron length polymorphism within the β‐tubulin genes and intron number variation among the α‐tubulin gene: three or four introns are found in two or four genes, respectively. Intron positioning occurs at conservative sites, as observed in numerous other plant species. Flax actin genes show both intron length polymorphisms and variation in the number of intron that may be two or three. These data will be useful to support further studies on the specificity, functioning, regulation, and evolution of the flax cytoskeleton proteins.
Multimodal 19F NMR Dopamine Detection and Imaging with a Nanoparticle‐Based Displacement Assay
Authors: Gabrielli Luca , Carril Monica , Padro Daniel , Mancin Fabrizio 
Published: June 2018

AbstractNanoconjugates composed of gold nanoparticles (core diameter=1.9 nm) coated with thioundecyl‐d‐glucopyranosides and fluorinated phenylboronic acids can detect diol‐containing derivatives by means of 19F NMR spectroscopic analysis. The spectra of nanoconjugate solutions display broad signals due to the fast relaxation of the 19F nuclei caused by nanoparticle grafting. When dopamine is added, the formation of a boronate ester between the analyte and the fluorinated boronic acid causes the release of the latter in solution and consequent sharpening of the NMR signals. Dopamine can be selectively detected through magnetic resonance imaging (MRI) and NMR spectroscopic analysis with respect to glucose and galactose with a detection limit of 20 μm. The chemical shift of the released ester is diagnostic of the recognized analyte. Consequently, the sensor also enables the simultaneous detection of different analytes.
Metal Free Mono‐ and 2,3‐Bis‐sulfenylation of Indoles in Water with Sodium Sulfinates as a Sulfur Source
Authors: Liu Changqing , Fan Jian , Wu Manyi , Chen Jiahui , Zhao Yiming , Xie Meihua 
Published: June 2018

AbstractAn iodine‐PPh3 mediated sulfenylation of indoles in water with stable and odorless sodium sulfinates as the sulfur source is described. The reaction could afford monosulfenylated indoles in moderate to excellent yields under metal free conditions. Moreover, double C—H sulfenylation of indoles at 2‐ and 3‐positions has also been achieved by using excess sodium sulfinates under the optimized reaction conditions.
Laser‐Mediated Photo‐High‐p,T Intensification of Vitamin D3 Synthesis in Continuous Flow
Authors: Escribà‐Gelonch Marc , Halpin Alexei , Noël Timothy , Hessel Volker 
Published: June 2018

AbstractA new reactor design for photosynthesis in microflow is presented based on spatial overlap of a laser beam through a capillary tube. A conical Archimedean spiral facilitates exposure of the entire volume of the reaction solution to the irradiation beam. In this fashion, photo‐high‐p,T intensification of the photolysis of 7‐dehydrocholesterol is achieved using either a pulsed ultraviolet (UV) laser excitation (in the order of femtoseconds) alone or in tandem with a conventional UV lamp. The goal is to control the kinetics of the side photoreactions under pulsed photolysis, as well as to reduce the reaction time and enhance the vitamin D3 yield. Three kinds of effects are targeted: a) Utilizing a very large and spatially homogeneous photon flux inside the flowing solution; b) the novel process window of combining photo‐ with thermal intensification to promote the final, irreversible thermal step to vitamin D3; and c) the use of sub‐ps laser pulses to supply excitation energy on a timescale shorter than the formation of side photoproducts. Finally, photo‐processing with the laser alone is compared to the tandem irradiation by the laser‐UV lamp.
Mechanistic Studies of the Cu(OH)+‐Catalyzed Isomerization of Glucose into Fructose in Water
Authors: Mensah Joel B. , Delidovich Irina , Hausoul Peter J. C. , Weisgerber Laurent , Schrader Wolfgang , Palkovits Regina 
Published: June 2018

AbstractThe isomerization of glucose to fructose is a crucial interim step in the processing of biomass to renewable fuels and chemicals. This study investigates the copper‐catalyzed glucose–fructose isomerization in water, focusing on insights into the roles of the dissolved copper species. Depending on the pH, the thermodynamic equilibrium shifted towards one or a few copper species, namely Cu2+, Cu(OH)+, and Cu(OH)2. According to thermodynamics, the highest concentration of Cu(OH)+ is at pH 5.3, at which the highest fructose yield of 16 % is achieved. The obtained fructose yields strongly correlate with the concentration of Cu(OH)+. A pH decrease of 2–3 units was observed during the reaction, resulting in the deactivation of the catalyst through hydrolysis in acidic media. Based on the results of the catalytic experiments, as well as spectroscopic and spectrometric studies, we propose Cu(OH)+ as an active Lewis‐acidic species following an intramolecular 1,2‐hydride shift.
Squeezing out the last egg—annual fish increase reproductive efforts in response to a predation threat
Authors: Grégoir Arnout Francis , Thoré Eli Samuel Joachim , Philippe Charlotte , Pinceel Tom , Brendonck Luc , Vanschoenwinkel Bram 
Published: June 2018

AbstractBoth constitutive and inducible antipredator strategies are ubiquitous in nature and serve to maximize fitness under a predation threat. Inducible strategies may be favored over constitutive defenses depending on their relative cost and benefit and temporal variability in predator presence. In African temporary ponds, annual killifish of the genus Nothobranchius are variably exposed to predators, depending on whether larger fish invade their habitat from nearby rivers during floods. Nonetheless, potential plastic responses to predation risk are poorly known. Here, we studied whether Nothobranchius furzeri individuals adjust their life history in response to a predation threat. For this, we monitored key life history traits in response to cues that signal the presence of predatory pumpkinseed sunfish (Lepomis gibbosus). While growth rate, adult body size, age at maturation, and initial fecundity were not affected, peak and total fecundity were higher in the predation risk treatment. This contrasts with known life history strategies of killifish from permanent waters, which tend to reduce reproduction in the presence of predators. Although our results show that N. furzeri individuals are able to detect predators and respond to their presence by modulating their reproductive output, these responses only become evident after a few clutches have been deposited. Overall our findings suggest that, in the presence of a predation risk, it can be beneficial to increase the production of life stages that can persist until the predation risk has faded.
Life‐cycle responses of a Mediterranean non‐migratory cyprinid species, the Northern Iberian chub (Squalius carolitertii Doadrio, 1988), to streamflow regulation
Authors: Alexandre Carlos M. , Ferreira Maria T. , Almeida Pedro R. 
Published: June 2018

AbstractStreamflow is considered a driver of interspecific and intraspecific life‐history differences among freshwater fish. Therefore, dams and related flow regulation can have deleterious impacts on their life cycles. The main objective of this study was to assess existing differences in the growth and reproduction patterns of a non‐migratory fish species (the Northern Iberian chub, Squalius carolitertii, Doadrio, 1988), between nonregulated and regulated watercourses. For 1 year, samples were collected from two populations of Iberian chub, inhabiting rivers with nonregulated and regulated flow regimes. Flow regulation for water storage promoted changes in chub's condition, duration of spawning, fecundity, and oocyte size. However, this non‐migratory species was less responsive to streamflow regulation than other native potamodromous species. Findings from this study are important to understand changes imposed by regulated rivers and can be used to support the implementation of suitable river management practices.