bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute high-intensity aerobic exercise affects brain-derived neurotrophic factor in mild cognitive impairment: a randomised controlled study
Authors: Devenney Kate E , Guinan Emer M , Kelly Áine M , Mota Bibiana C , Walsh Cathal , Olde Rikkert Marcel , Schneider Stefan , Lawlor Brian 
Published: June 2019

ObjectiveTo investigate the brain-derived neurotrophic factor (BDNF) and cognitive response to a short bout of high-intensity aerobic exercise in older adults with mild cognitive impairment (MCI).MethodsParticipants were randomised to one of two testing schedules, completing either a standardised exercise test (group A) or a resting control condition (group B). Blood sampling and cognitive measures (visuospatial learning and memory, sustained attention and executive function) were collected at baseline (T1) and postintervention (T2). An additional measurement of study outcomes was collected after exercise (T3) in group B only.Results64 participants (female 53.2%, mean age 70.5±6.3 years) with MCI were recruited. From T1 to T2, serum BDNF (sBDNF) concentration increased in group A (n=35) (median (Md) 4564.61±IQR 5737.23 pg/mL to Md 5173.27±5997.54 pg/mL) and decreased in group B (Md 4593.74±9558.29 pg/mL to Md 3974.66±3668.22 pg/mL) (between-group difference p=0.024, effect size r=0.3). The control group made fewer errors on the sustained attention task compared with the exercise group (p=0.025). Measures of visuospatial learning and memory or executive function did not change significantly between groups.ConclusionThis study is the first to show that a short bout of high-intensity aerobic exercise increases peripheral sBDNF in a population with MCI. However, acute exercise did not improve cognitive performance.
Risk factors for longitudinal changes in left ventricular diastolic function among women and men
Authors: Rueda-Ochoa Oscar L , Smiderle-Gelain Marco A , Rizopoulos Dimitris , Dhana Klodian , van den Berge Jan-Kees , Echeverria Luis E , Ikram M Arfan , Deckers Jaap W , Franco Oscar H , Kavousi Maryam 
Published: June 2019

ObjectiveTo evaluate changes in left ventricular diastolic function (LVDF) parameters and their associated risk factors over a period of 11 years among community-dwelling women and men.MethodsEchocardiography was performed three times among 870 women and 630 men (age 67±3 years) from the prospective population-based Rotterdam Study during a period of 11-year follow-up. Changes in six continuous LVDF parameters were correlated with cardiovascular risk factors using a linear-mixed effect model (LMM).ResultsIn women, smoking was associated with deleterious longitudinal changes in deceleration time (DT) (Beta (β): 7.73; 95% CI 2.56 to 12.9) and high-density lipoprotein cholesterol was associated with improvement of septal e′ (β: 0.37; 95% CI 0.13 to 0.62) and E/e′ ratio (β: −0.46; 95% CI −0.84 to –0.08) trajectories. Among men, diabetes was associated with deleterious longitudinal changes in A wave (β: 3.83; 95% CI 0.06 to 7.60), septal e′ (β: −0.40; 95% CI −0.70 to –0.09) and E/e′ ratio (β: 0.60; 95% CI 0.14 to 1.06) and body mass index was associated with deleterious longitudinal changes in A wave (β: 1.25; 95% CI 0.84 to 1.66), E/A ratio (β: −0.007; 95% CI −0.01 to –0.003), DT (β: 0.86; 95% CI 0.017 to 1.71) and E/e′ ratio (β: 0.12; 95% CI 0.06 to 0.19).ConclusionsSmoking among women and metabolic factors (diabetes mellitus and body mass index) among men showed larger deleterious associations with longitudinal changes in LVDF parameters. The favourable association of HDL was mainly observed among women. This study, for the first time, evaluates risk factors associated with changes over time in continuous LVDF parameters among women and men and generates new hypothesis for further medical research.
Clinical effectiveness of single dose of intravenous dexamethasone on the duration of ropivacaine axillary brachial plexus block: the randomized placebo-controlled ADEXA trial
Authors: Clement Jean-Christophe , Besch Guillaume , Puyraveau Marc , Grelet Tommy , Ferreira David , Vettoretti Lucie , Pili-Floury Sébastien , Samain Emmanuel , Berthier Francis 
Published: June 2019

Background and objectivesThe effect of intravenous dexamethasone on the duration of axillary plexus block performed using ropivacaine is not described. The aim of this study is to assess the effect of intravenous dexamethasone on the duration of axillary plexus block analgesia after distal upper arm surgery.MethodsIn this prospective, randomized, placebo-controlled, double-blinded trial, consenting patients scheduled for hand or forearm surgery under ultrasound-guided axillary plexus block performed using 0.5 mL/kg of 0.475% ropivacaine, were randomized to receive an intravenous injection of either 8 mg/2 mL of dexamethasone (Dexa group) or 2 mL of saline (Control). The primary outcome was the time of first analgesic intake after axillary block. Secondary outcomes included motor or sensory block duration, total use of postoperative analgesics, and block-related complications.ResultsAmong the 98 patients included, 6 and 2 patients did not require postoperative analgesic intake in Dexa and Control groups, respectively (p=0.06). The time of first analgesic intake was significantly longer in the Dexa (20.9±9.3 hours) than in the Control group (14.7±6.6 hours, p<0.0004). Motor and sensory recovery occurred significantly later, and total analgesic consumption was lower in the Dexa than in the Control group. No nerve complication related to intravenous dexamethasone injection was recorded.ConclusionsThis study showed that intravenous dexamethasone delayed for 6 hours the time to first analgesic intake after upper arm surgery under axillary plexus block performed with the long-lasting local anesthetic ropivacaine. This suggests that intravenous dexamethasone could be an interesting adjuvant to axillary plexus block.Trial registration numberNCT02862327
Factors influencing Danish veterinarians’ choice of antimicrobials prescribed for intestinal diseases in weaner pigs
Authors: Eriksen Esben Oestergaard , Smed Simon , Klit Karl Johan , Olsen John Elmerdahl 
Published: June 2019

BackgroundAntimicrobial resistance is a worldwide human and animal health problem, and there is an urgent need to promote prudent use of antimicrobials among veterinarians. In order to do so, it is important to understand the factors that determine their use of antimicrobials. This questionnaire‐based study aimed to determine which factors that influence the Danish veterinarians’ choice of antimicrobials prescribed for intestinal diseases in weaner pigs.MethodsThe survey was completed by 83.3 per cent (n=105) of all veterinarians accountable for a Veterinary Advisory Contract in Danish weaner pig herds (n=126). The participants scored to which extent 29 different factors influenced their antimicrobial choice on a five‐point Likert scale (1‐5).ResultsThe veterinarian's own experiences of clinical efficacy in the herd exerted the greatest influence (94.4 per cent scored ≥4). The Danish authorities have directed a threshold of the antimicrobial use and made some antimicrobials less favourable to use in pig production through The Yellow Card Initiative, and this influenced the choice of antimicrobials significantly (78.1 per cent scored ≥4). Microbiological laboratory diagnostics influenced the choice of antimicrobial for most veterinarians (78.1 per cent scored 4 or 5), and therefore the Danish statutory requirement of laboratory diagnostics before flock treatment was considered reasonable.ConclusionThe study concluded that many factors influenced the veterinarians choice of antimicrobials, and that statutory requirments can be used to support prudent use of antimicrobials.
Comparison between hepaticojejunostomy and hepaticoduodenostomy after excision of choledochal cyst in children: a cohort study
Authors: Guzman Johann Paulo Suico , Resurreccion III Leandro L , Suntay Marcus Lester R , Bernaldez Renato G 
Published: June 2019

ObjectiveHepaticojejunostomy (HJ) and hepaticoduodenostomy (HD) are commonly used biliary reconstruction techniques after choledochal cyst excision. HD has been suggested to be a more physiologic alternative during reconstruction. The objective of this study is to compare operative time, hospital stay, morbidity (leak, cholangitis, ileus, and obstruction), and mortality between HJ and HD after cyst excision.MethodsThis is a 14-year retrospective cohort study of pediatric patients (≤18 years old) who underwent choledochal cyst excision and subsequent biliary reconstruction at the Philippine Children’s Medical Center. Data were taken from inpatient charts, operative technique, OPD logbook, readmission, and OPD charts.ResultsThere were 122 patients: 56% HD and 44% HJ. Majority were female (72%), with 1:2.6 male to female ratio. The average age was 36.1 months, with a mean follow-up of 32.8 months (range 6 months–14 years). The most common cyst was type I (87%). Operative time was longer for HJ compared with HD (321.3 vs 203.6 min; p=0.000). Hospital stay was longer with HJ compared with HD (7.7 vs 6.8 days; p=0.002). Mortality rate was low at 1.6% while morbidity was at 13.9% in both groups. Although morbidity was higher among those who underwent HD, there was no significant difference between the two procedures. Anastomotic leak (4%) and cholangitis (7.4%) were observed in HD, and ileus (7.4%) was observed in the HJ group.ConclusionsIn our series, HD provided less operative time and hospital stay than with HJ. We did not observe bile gastritis after HD as compared with others. It is suggested that longer follow-up is needed to confirm such findings.
Centrality and the shortest path approach in the human interactome
Authors: Rubanova Natalia , Morozova Nadya 
Published: June 2019

Many notions and concepts for network analysis, including the shortest path approach, came to systems biology from the theory of graphs — the field of mathematics that studies graphs. We studied the relationship between the shortest paths and a biologically meaningful molecular path between vertices in human molecular interaction networks. We analyzed the sets of the shortest paths in the human interactome derived from HPRD and HIPPIE databases between all possible combinations of start and end proteins in eight signaling pathways in the KEGG database — NF-kappa B, MAPK, Jak-STAT, mTOR, ErbB, Wnt, TGF-beta, and the signaling part of the apoptotic process. We investigated whether the shortest paths match the canonical paths. We studied whether centrality of vertices and paths in the subnetworks induced by the shortest paths can highlight vertices and paths that are part of meaningful molecular paths. We found that the shortest paths match canonical counterparts only for canonical paths of length 2 or 3 interactions. The shortest paths match longer canonical counterparts with shortcuts or substitutions by protein complex members. We found that high centrality vertices are part of the canonical paths for up to 80% of the canonical paths depending on the database and the length.
LiwePMS
Authors: Li Sumin , Huang Kaixin , Huang Linpeng , Zhu Jiashun 
Published: June 2019

Next-generation Storage Class Memory (SCM) offers low-latency, high-density, byte-addressable access and persistency. The potent combination of these attractive characteristics makes it possible for SCM to unify the main memory and storage to reduce the storage hierarchy. Aiming for this, several persistent memory systems were designed. However, the heavy metadata and transaction cost degrade the system performance. Moreover, neither of them pays attention to wear-leveling strategy.
In this article, we present a lightweight persistent memory system, LiwePMS, which allows a fast access to persistent data stored in SCM with wear-aware memory management. LiwePMS makes performance improvement by simplifying the metadata management and the consistency method. LiwePMS abstracts SCM as heap space with container-based dynamic address mapping. Also, LiwePMS implements efficient wear-aware dynamic memory allocator and lightweight transaction mechanism for data consistency in user-space library. The experiments showed that LiwePMS persists key-value records 1.5× faster than Redis RDB mechanism. LiwePMS improves the performance of persistent region operation by more than 45%, 63%, and 1.1× comparing with HEAPO, Mnemosyne, and NVML, respectively. Also, the wear-leveling policy of memory allocator outperforms that of NVMalloc from 35% to 30%, and the transaction method promotes the transaction performance to 1.8× compared to NVML.
SUMO-Specific Protease 1 Is Critical for Myeloid-Derived Suppressor Cell Development and Function
Authors: Huang Xian , Zuo Yong , Wang Xiuzhi , Wu Xuefeng , Tan Hongsheng , Fan Qiuju , Dong Baijun , Xue Wei , Chen Guo-Qiang , Cheng Jinke 
Published: June 2019

Abstract

Myeloid-derived suppressor cells (MDSC) can suppress immunity and promote tumorigenesis, and their abundance is associated with poor prognosis. In this study, we show that SUMO1/sentrin-specific peptidase 1 (SENP1) regulates the development and function of MDSC. SENP1 deficiency in myeloid cells promoted MDSC expansion in bone marrow, spleen, and other organs. Senp1−/− MDSC showed stronger immunosuppressive activity than Senp1+/+ MDSC; we observed no defects in the differentiation of myeloid precursor cell in Senp1−/− mice. Mechanistically, SENP1-mediated regulation of MDSC was dependent on STAT3 signaling. We identified CD45 as a specific STAT3 phosphatase in MDSC. CD45 was SUMOylated in MDSC and SENP1 could deconjugate SUMOylated CD45. In Senp1−/− MDSC, CD45 was highly SUMOylated, which reduced its phosphatase activity toward STAT3, leading to STAT3-mediated MDSC development and function. These results reveal a suppressive function of SENP1 in modulating MDSC expansion and function via CD45–STAT3 signaling axis.

Significance:
These findings show that increased SUMOylation of CD45 via loss of SENP1 suppresses CD45-mediated dephosphorylation of STAT3, which promotes MDSC development and function, leading to tumorigenesis.
Serum Levels of 25-Hydroxyvitamin D at Diagnosis Are Not Associated with Overall Survival in Esophageal Adenocarcinoma
Authors: Loehrer Elizabeth , Betensky Rebecca A. , Giovannucci Edward , Su Li , Shafer Andrea , Hollis Bruce W. , Christiani David C. 
Published: June 2019

Abstract

Background:
Higher levels of circulating 25-hydroxyvitamin D [25(OH)D] are associated with longer survival in several cancers, but the results have differed across cancer sites. The association between serum 25(OH)D levels and overall survival (OS) time in esophageal adenocarcinoma remains unclear.

Methods:
We utilized serum samples from 476 patients with primary esophageal adenocarcinoma, recruited from Massachusetts General Hospital (Boston, MA) between 1999 and 2015. We used log-rank tests to test the difference in survival curves across quartiles of 25(OH)D levels and extended Cox modeling to estimate adjusted HRs. We tested for interactions between clinical stage or BMI on the association between 25(OH)D and OS. We additionally performed sensitivity analyses to determine whether race or timing of blood draw (relative to treatment) affected these results.

Results:
We found no evidence that survival differed across quartiles of 25(OH)D (log rank P = 0.48). Adjusting for confounders, we found no evidence that the hazard of death among the highest quartile of 25(OH)D (quartile 1) differed from any other quartile [quartile 2 HR = 0.90, 95% confidence interval (CI), 0.67–1.23; quartile 3 HR = 1.03, 95% CI, 0.76–1.38; quartile 4 (lowest) HR = 0.98, 95% CI, 0.72–1.33]. Sensitivity analyses yielded consistent results when accounting for race or time between diagnosis and blood draw. Moreover, we did not find evidence of interaction between 25(OH)D and clinical stage or BMI on OS.

Conclusions:
Serum level of 25(OH)D near time of diagnosis was not associated with OS in patients with esophageal adenocarcinoma.

Impact:
Screening 25(OH)D levels among patients with esophageal adenocarcinoma at diagnosis is not clinically relevant to their cancer prognosis based on present evidence.
Dietary Acrylamide Intake and Risk of Esophageal, Gastric, and Colorectal Cancer: The Japan Public Health Center–Based Prospective Study
Authors: Liu Rong , Sobue Tomotaka , Kitamura Tetsuhisa , Kitamura Yuri , Ishihara Junko , Kotemori Ayaka , Zha Ling , Ikeda Sayaka , Sawada Norie , Iwasaki Motoki , Tsugane Shoichiro 
Published: June 2019

Abstract

Background:
Acrylamide has been classified as a probable human carcinogen based chiefly on laboratory evidence. However, the influence of dietary acrylamide intake on risk of esophageal, gastric, and colorectal cancer has not been extensively studied. We aimed to evaluate the association between dietary acrylamide intake and esophageal, gastric, and colorectal cancer using data from the Japan Public Health Center–based Prospective Study.

Methods:
Our study included 87,628 participants who completed a food-frequency questionnaire at enrollment in 1990 and 1993. We used Cox proportional hazards regression models to estimate hazards ratios and 95% confidence intervals (CI) after adjusting for confounding factors.

Results:
After 15.5, 15.3, and 15.3 mean years of follow-up for esophageal, gastric, and colorectal cancer, we identified and analyzed 391 esophageal, 2,218 gastric, and 2,470 colorectal cancer cases, respectively. Compared with the lowest quintile of acrylamide intake, the multivariate HR for the highest quintile was 0.86 (95% CI, 0.53–1.39; Ptrend = 0.814), 0.84 (95% CI, 0.69–1.01; Ptrend = 0.301), and 0.93 (95% CI, 0.79–1.08; Ptrend = 0.165) for esophageal, gastric, and colorectal cancer, respectively, in the multivariable-adjusted model. Furthermore, no significant associations were observed when the participants were stratified by cancer subsites.

Conclusions:
In conclusion, this study demonstrated that dietary acrylamide intake was not associated with increased risk of esophageal, gastric, or colorectal cancer among the Japanese population.

Impact:
It is the first time to assess the effect of dietary acrylamide intake on risk of digestive system cancer in Asian populations.
Incidence of Ductal Carcinoma In Situ in the United States, 2000–2014
Authors: Ryser Marc D. , Hendrix Laura H. , Worni Mathias , Liu Yiling , Hyslop Terry , Hwang E. Shelley 
Published: June 2019

Abstract

Background:
In absence of definitive molecular risk markers, clinical management of patients diagnosed with ductal carcinoma in situ (DCIS) remains largely guided by patient and tumor characteristics. In this study, we analyzed recent trends in DCIS incidence and compared them against trends in mammography use.

Methods:
The Surveillance, Epidemiology, and End Results registry was queried for patients diagnosed with DCIS from 2000 to 2014 (18 registries). Joinpoint regression analyses were used to compute age- and race-stratified trends in age-adjusted incidence of DCIS. The patterns of DCIS incidence were compared against mammography utilization data from the National Health Interview Survey.

Results:
Between 2000 and 2014, overall DCIS incidence in the U.S. population was stable (P = 0.24). Among age groups 20 to 44 years and 45 to 55 years, DCIS incidence increased by 1.3% (P = 0.001) and 0.6% (P = 0.02) per year, respectively. Although stable among white women, DCIS incidence increased among black women and women of other races by 1.6% (P &lt; 0.001) and 1.0% (P = 0.002) per year, respectively. Mammography uptake correlated well with DCIS incidence, with the exception of women ages 40 to 49 years and black women who experienced an increase in DCIS incidence despite stagnating and decreasing mammography uptake, respectively.

Conclusions:
Overall DCIS incidence rates have remained stable between 2000 and 2014. However, subgroup analyses revealed an increase in incidence among both younger women and black women.

Impact:
DCIS incidence trends did not correlate with the mammography uptake patterns, suggesting that etiologic factors other than screening may be leading to an increased DCIS incidence in these groups.
Tumor Lymphatic Function Regulates Tumor Inflammatory and Immunosuppressive Microenvironments
Authors: Kataru Raghu P. , Ly Catherine L. , Shin Jinyeon , Park Hyeung Ju , Baik Jung Eun , Rehal Sonia , Ortega Sagrario , Lyden David , Mehrara Babak J. 
Published: June 2019

Abstract
Proliferation of aberrant, dysfunctional lymphatic vessels around solid tumors is a common histologic finding. Studies have shown that abnormalities in lymphatic function result in accumulation of inflammatory cells with an immunosuppressive profile. We tested the hypothesis that dysfunctional lymphatic vessels surrounding solid tumors regulate changes in the tumor microenvironment and tumor-specific immune responses. Using subcutaneously implanted mouse melanoma and breast cancer tumors in a lymphatic endothelial cell–specific diphtheria toxin receptor transgenic mouse, we found that local ablation of lymphatic vessels increased peritumoral edema, as compared with controls. Comparative analysis of the peritumoral fluid demonstrated increases in the number of macrophages, CD4+ inflammatory cells, F4/80+/Gr-1+ (myeloid-derived suppressor cells), CD4+/Foxp3+ (Tregs) immunosuppressive cells, and expression of inflammatory cytokines such as TNFα, IFNγ, and IL1β following lymphatic ablation. Tumors grown in lymphatic ablated mice exhibited reduced intratumoral accumulation of cytotoxic T cells and increased tumor PD-L1 expression, causing rapid tumor growth, compared with tumors grown in nonlymphatic-ablated mice. Our study suggests that lymphatic dysfunction plays a role in regulating tumor microenvironments and may be therapeutically targeted in combination with immunotherapy to prevent tumor growth and progression.
An Improved Patient-Derived Xenograft Humanized Mouse Model for Evaluation of Lung Cancer Immune Responses
Authors: Meraz Ismail M. , Majidi Mourad , Meng Feng , Shao RuPing , Ha Min Jin , Neri Shinya , Fang Bingliang , Lin Steven H. , Tinkey Peggy T. , Shpall Elizabeth J. , Morris Jeffrey , Roth Jack A. 
Published: June 2019

Abstract
Human tumor xenograft models do not replicate the human immune system and tumor microenvironment. We developed an improved humanized mouse model, derived from fresh cord blood CD34+ stem cells (CD34+ HSC), and combined it with lung cancer cell line–derived human xenografts or patient-derived xenografts (Hu-PDX). Fresh CD34+ HSCs could reconstitute detectable mature human leukocytes (hCD45+) in mice at four weeks without the onset of graft-versus-host disease (GVHD). Repopulated human T cells, B cells, natural killer (NK) cells, dendritic cells (DC), and myeloid-derived suppressor cells (MDSC) increased in peripheral blood, spleen, and bone marrow over time. Although cultured CD34+ HSCs labeled with luciferase could be detected in mice, the cultured HSCs did not develop into mature human immune cells by four weeks, unlike fresh CD34+ HSCs. Ex vivo, reconstituted T cells, obtained from the tumor-bearing humanized mice, secreted IFNγ upon treatment with phorbol myristate acetate (PMA) or exposure to human A549 lung tumor cells and mediated antigen-specific CTL responses, indicating functional activity. Growth of engrafted PDXs and tumor xenografts was not dependent on the human leukocyte antigen status of the donor. Treatment with the anti–PD-1 checkpoint inhibitors pembrolizumab or nivolumab inhibited tumor growth in humanized mice significantly, and correlated with an increased number of CTLs and decreased MDSCs, regardless of the donor HLA type. In conclusion, fresh CD34+HSCs are more effective than their expanded counterparts in humanizing mice, and do so in a shorter time. The Hu-PDX model provides an improved platform for evaluation of immunotherapy.