bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell-free MicroRNA miR-505-3p in Graft Preservation Fluid Is an Independent Predictor of Delayed Graft Function After Kidney Transplantation
Authors: Roest Henk P. , Ooms Liselotte S.S. , Gillis Ad J.M. , IJzermans Jan N.M. , Looijenga Leendert H.J. , Dorssers Lambert C.J. , Dor Frank J.M.F. , van der Laan Luc J.W. 
Published: November 2018

Background
Delayed graft function (DGF), a common complication after transplantation of deceased donor kidneys, affects both short- and long-term outcomes. Currently available biomarkers during graft preservation lack sensitivity in predicting risk for DGF. The aim of this study is to identify cell-free micro ribonucleic acid (miRNA) biomarkers in graft preservation fluid predictive of DGF after kidney transplantation.

Methods
Vascular bed preservation fluid was collected from 48 kidney grafts from donation after circulatory death (DCD) or donation after brain death (DBD) donors. miRNA profiles were determined by polymerase chain reaction (PCR) array (n = 8) and validated by reverse transcription and quantitative PCR (n = 40). Graft function posttransplantation was defined as immediate good function (IF) or DGF.

Results
A total of 223 miRNAs fulfilled the preset parameters (Ct < 40 in 3 or more samples) and were included in the analysis. Thirty-two miRNAs were significantly different between DGF and IF kidney grafts (P < 0.05) but, after correction for multiple testing, only miR-505-3p remained significant. The significant association of high miR-505-3p levels with DGF was confirmed in an independent validation cohort using conventional reverse transcription and quantitative PCR detection. Multivariate analyses showed miR-505-3p as an independent predictor for DGF (odds ratio, 1.12; P = 0.028). If stratified for donor type, miR-505-3p levels remained significantly different between IF and DGF in DCD grafts (P < 0.01), but not in DBD grafts. Receiver operating characteristic curve analysis showed a high sensitivity and specificity (area under the curve, 0.833).

Conclusions
In DCD grafts, high levels of miR-505-3p in preservation fluid are associated with increased risk of DGF after kidney transplantation. Further study is required to confirm the utility of cell-free miR-505-3p as prognostic biomarker for DGF.
A Propensity-matched Survival Analysis: Do Simultaneous Liver-lung Transplant Recipients Need a Liver?
Authors: Freischlag Kyle , Ezekian Brian , Schroder Paul M. , Mulvihill Michael S. , Cox Morgan L. , Hartwig Matthew G. , Knechtle Stuart 
Published: November 2018

Background.There is debate whether simultaneous lung-liver transplant (LLT) long-term outcomes warrant allocation of 2 organs to a single recipient. We hypothesized that LLT recipients would have improved posttransplant survival compared with matched single-organ lung recipients with an equivalent degree of liver dysfunction.Methods.The Organ Procurement and Transplant Network/United Network for Organ Sharing STAR file was queried for adult candidates for LLT and isolated lung transplantation from 2006 to 2016. Waitlist mortality and transplant odds were calculated for all candidates. Donor and recipient demographic characteristics were compiled and compared. The LLT recipients were matched 1:2 with a nearest neighbor method to single-organ lung recipients. Kaplan-Meier methods with log-rank test compared long-term survival between groups. Univariate regression was used to calculate the association of LLT and mortality within 6 months of transplant. A proportional hazards model was used to calculate risk-adjusted mortality after 6 months posttransplantation.Results.Thirty-eight LLT patients were matched to 75 single-organ lung recipients. After matching, no differences in baseline demographics or liver function were observed between cohorts. Length of stay was significantly longer in LLT recipients compared to isolated lung recipients (45.89 days vs 22.44 days,P< 0.001). There was no significant difference in survival probability between LLT and isolated lung transplant (1 y, 89.5% vs 86.7%; 5 y, 67.0% vs 64.6%;P= 0.20).Conclusions.After matching for patient characteristics and level of liver dysfunction, survival in simultaneous LLT was comparable to isolated lung transplantation. Although this population is unique, the clinical picture prompting liver transplant is not clear. National guidelines to better elucidate patient selection are needed.
Recipient Outcomes From Nondirected Live Kidney Donors: A UK-based Cohort Study
Authors: Nath Jay , Patel Kamlesh , Field Melanie , Hodson James , Sharif Adnan , Inston Nicholas G. , Ready Andrew R. 
Published: November 2018

BackgroundIncreasing numbers of patients with end-stage renal failure are receiving kidneys from nondirected kidney donors (NKDs), also known as altruistic donors. Transplant outcomes for recipients of such kidneys are largely inferred from studies on specified kidney donors (SKDs), which may be inaccurate due to differences in donor, recipient and transplant specific factors. We report the outcomes for recipients of NKD in the United Kingdom.MethodsOutcomes for 6861 patients receiving a living donor kidney transplant between January 2007 and December 2014 were analyzed using both the National Health Service Blood and Transplant and the UK Renal Registry datasets. Graft and patient outcomes were compared for patients receiving NKD and SKD organs using univariable and multivariable analyses.ResultsThere was significant discordance between the NKD and SKD donors and recipients. These included increased donor age (median, 58 years vs 47 years;P< 0.001) and higher rates of hemodialysis and previous transplants in the NKD group (bothP< 0.001). Despite such markers of increased risk among both donors and recipients of NKD kidneys, there was no difference in graft survival on univariable (hazard ratio, 1.20; 95% confidence interval, 0.77-1.86;P= 0.419) or multivariable analysis (hazard ratio, 1.13; 95% confidence interval, 0.65-1.95;P= 0.665).ConclusionsDespite some markers of transplant complexity, nondirected kidney donor organs are an excellent source of organs for transplantation.
Vestibular rehabilitation: advances in peripheral and central vestibular disorders
Authors: Dunlap Pamela M. , Holmberg Janene M. , Whitney Susan L. 
Published: November 2018

Purpose of review
Rehabilitation for persons with vertigo and balance disorders is becoming commonplace and the literature is expanding rapidly. The present review highlights recent findings of both peripheral and central vestibular disorders and provides insight into evidence related to new rehabilitative interventions. Risk factors will be reviewed to create a better understanding of patient and clinical characteristics that may effect recovery among persons with vestibular disorders.

Recent findings
Clinical practice guidelines have recently been developed for peripheral vestibular hypofunction and updated for benign paroxysmal positional vertigo. Diagnoses such as persistent postural-perceptual dizziness (PPPD) and vestibular migraine are now defined, and there is growing literature supporting the effectiveness of vestibular rehabilitation as a treatment option. As technology advances, virtual reality and other technologies are being used more frequently to augment vestibular rehabilitation. Clinicians now have a better understanding of rehabilitation expectations and whom to refer based on evidence in order to improve functional outcomes for persons living with peripheral and central vestibular disorders.

Summary
An up-to-date understanding of the evidence related to vestibular rehabilitation can assist the practicing clinician in making better clinical decisions for their patient and hopefully result in optimal functional recovery.
Is intravenous thrombolysis still necessary in patients who undergo mechanical thrombectomy?
Authors: Katsanos Aristeidis H. , Tsivgoulis Georgios 
Published: November 2018

Purpose of review
To summarize available evidence on the potential utility of pretreatment with intravenous thrombolysis (IVT) using recombinant tissue-plasminogen activator (rt-PA) in acute ischemic stroke (AIS) patients with large vessel occlusions (LVO) who are treated with mechanical thrombectomy.

Recent findings
Despite theoretical concerns of a higher bleeding risk with IVT pretreatment, there are no data showing increased risk of symptomatic intracerebral hemorrhage (sICH) in patients with LVO receiving bridging therapy (IVT and mechanical thrombectomy) compared with direct mechanical thrombectomy (dMT). Additionally, evidence from observational studies suggest lower rates of infarctions in previously unaffected territories and higher rates of successful reperfusion, with lower number of device passes, in patients receiving bridging therapy. There are substantial discrepancies in studies comparing clinical outcomes between dMT and bridging therapy that are directly related to the inclusion of patients with contraindications to IVT in the dMT group. Ongoing clinical trials will provide definitive answers on the potential additional benefit of IVT in LVO patients receiving mechanical thrombectomy.

Summary
IVT and mechanical thrombectomy are two effective reperfusion therapies that should be used in a swift and noncompeting fashion in AIS patients. AIS patients with LVO and no contraindications for IVT should receive promptly rt-PA bolus followed by immediate initiation of mechanical thrombectomy as indicated by current international recommendations, unless future randomized controlled trials provide evidence to proceed differently.
New concepts on acute ocular ischemia
Authors: Sharma Rahul A. , Newman Nancy J. , Biousse Valérie 
Published: November 2018

Purpose of review
To review recent advances in the management of acute ocular ischemic events, including: transient monocular vision loss, central and branch retinal artery occlusions, and nonarteritic anterior ischemic optic neuropathy.

Recent findings
Transient monocular vision loss and acute retinal arterial occlusions require immediate diagnosis and management, with recognition of these events as transient ischemic attack or stroke equivalents, respectively. Patients should undergo an immediate stroke workup in a stroke center, similar to patients with acute cerebral ischemia. The treatment of central retinal artery occlusions remains limited despite the growing use of thrombolytic treatments. The indication for these treatments remains under debate. No quality evidence exists to support any therapy, including corticosteroids, in the treatment of nonarteritic anterior ischemic optic neuropathy. The highest priority in management is to rule-out giant cell arteritis.

Summary
Effective therapies for the treatment of ischemic events of the retina and optic nerve remain elusive. Clinicians should focus on the prompt recognition of these events as ocular emergencies and immediately refer patients with vascular transient visual loss and acute central and branch retinal arterial occlusions to the nearest stroke center.
Multidimensional dynamics of the proteome in the neurodegenerative and ageing mammalian brain
Authors: Andrews Byron , Maslen Sarah , Almeida-Souza Leonardo , Skehel J Mark , Frank René 
Published: November 2018

AbstractThe amount of any given protein in the brain is determined by the rates of its synthesis and destruction, which are regulated by different cellular mechanisms. Here, we combine metabolic labelling in live mice with global proteomic profiling to simultaneously quantify both the flux and amount of proteins in mouse models of neurodegeneration. In multiple models, protein turnover increases were associated with increasing pathology. This method distinguishes changes in protein expression mediated by synthesis from those mediated by degradation. In the AppNL-F knockin mouse model of Alzheimer’s disease increased turnover resulted from imbalances in both synthesis and degradation, converging on proteins associated with synaptic vesicle recycling (Dnm1, Cltc, Rims1) and mitochondria (Fis1, Ndufv1). In contrast to disease models, ageing in wildtype mice caused a widespread decrease in protein recycling associated with a decrease in autophagic flux. This simple multidimensional approach enables the comprehensive mapping of proteome dynamics and identifies affected proteins in mouse models of disease and other live animal test settings.
MRE11-RAD50-NBS1 activates Fanconi Anemia R-loop suppression at transcription-replication conflicts
Authors: Chang Emily Yun-chia , Wells James P. , Tsai Shu-Huei , Coulombe Yan , Chan Yujia A. , Zhu Yi Dan , Fournier Louis-Alexandre , Hieter Philip , Masson Jean-Yves , Stirling Peter C. 
Published: November 2018

SUMMARYEctopic R-loop accumulation causes DNA replication stress and genome instability. To avoid these outcomes, cells possess a range of anti-R-loop mechanisms, including RNaseH that degrades the RNA moiety in R-loops. To comprehensively identify anti-R-loop mechanisms, we performed a genome-wide trigenic interaction screen in yeast lacking RNH1 and RNH201. We identified >100 genes critical for fitness in the absence of RNaseH, which were enriched for DNA replication fork maintenance factors such as RAD50. We show in yeast and human cells that R-loops accumulate during RAD50 depletion. In human cancer cell models, we find that RAD50 and its partners in the MRE11-RAD50-NBS1 complex regulate R-loop-associated DNA damage and replication stress. We show that a non-nucleolytic function of MRE11 is important for R-loop suppression via activation of PCNA-ubiquitination by RAD18 and recruiting anti-R-loop helicases in the Fanconi Anemia pathway. This work establishes a novel role for MRE11-RAD50-NBS1 in directing tolerance mechanisms of transcription-replication conflicts.
Dynamic constriction and fission of ER membranes by reticulon
Authors: Espadas Javier , Pendin Diana , Bocanegra Rebeca , Escalada Artur , Misticoni Giulia , Trevisan Tatiana , Velasco del Olmo Ariana , Bova Sergio , Ibarra Borja , Kuzmin Peter I. , Shnyrova Anna V. , Frolov Vadim A. , Daga Andrea 
Published: November 2018

AbstractThe endoplasmic reticulum (ER) is a continuous cell-wide membrane network. Network formation has been widely associated with proteins producing membrane curvature and fusion, such as reticulons and atlastin. Regulated network fragmentation, occurring in different physiological contexts, is less understood. We found that the ER network has an embedded fragmentation mechanism based upon the ability of reticulons to produce fission of elongating network branches. In Drosophila, fission is counterbalanced by atlastin-driven fusion, with their imbalance leading to ER fragmentation. Live imaging of ER network dynamics upon ectopic expression of Drosophila reticulon linked fission to augmented membrane friction. Consistently, in vitro analysis revealed that purified reticulon produced velocity-dependent constriction and fission of lipid nanotubes pulled from a flat reservoir membrane. Fission occurred at elongation rates and pulling force ranges intrinsic to the ER network, thus suggesting a novel principle of organelle morphology regulation where the dynamic balance between fusion and fission is governed by membrane motility.
What makes me think that this product fits me? The impact of perceptual processing style on product preference among female consumers in emerging Asian countries
Authors: Charinsarn Alisara Rungnontarat 
Published: November 2018

PurposeThe purpose of this paper is to study whether the consumer’s perceptual processing style impacts consumer preference. Specifically, whether consumers with local perceptual processing style in emerging Asian countries are more likely to perceive that Asian skincare brands are suitable for them, compared with emerging Asian consumers with global perceptual processing.Design/methodology/approachAn experiment was conducted with 249 Asian female respondents in an emerging Asian market. The Navon letter – a hierarchical letter – is used to classify the respondents’ perceptual processing style. The Navon stimulus used is a big letter H made up of small letter Ls. Those who saw letter H (L) at first glance were classified as consumers with global (local) perceptual processing style. ANCOVA was used to analyze the main effect of global/local perceptual processing style on product preference, as well as to test the moderating effect of age.FindingsThe results suggest that consumers with local perceptual processing style are significantly more likely to perceive that Asian brands are suitable for them, compared with respondents with global perceptual processing style. Additionally, the covariate “age” is not the covariate between global/local perceptual processing and skincare preference.Practical implicationsManagers can apply the findings to their targeting and communication strategies. First, it will be advantageous for managers marketing Asian skincare products to target Asian consumers with local perceptual processing style. For managers marketing Western skincare products in Asia, it will be better to target Asian consumers with global perceptual processing style. In terms of communication strategy development, managers marketing Asian skincare products to Asian consumers are recommended to encourage local perceptual processing, while managers marketing Western skincare brands to emerging Asian consumers should encourage global perceptual processing.Originality valueThe value of this paper is that it applies the perceptual processing style to explain consumer decision-making in the context of consumers in emerging Asian countries. It also highlights a new perspective that helps managers craft targeting and communication strategies that help make their products to be perceived as a better fit or to facilitate consumer processing style so the product becomes a preferred choice.
Demographic and clinical characteristics of spinal calcinosis in systemic sclerosis: Possible association with peripheral angiopathy
Authors: Motegi Sei‐ichiro , Sekiguchi Akiko , Yonemoto Yukio , Mieda Tokue , Chikuda Hirotaka , Ishikawa Osamu 
Published: November 2018

AbstractThe objective was to evaluate the demographic and clinical characteristics of systemic sclerosis (SSc) patients with spinal calcinosis. Paraspinal and intraspinal calcinosis was assessed blindly by orthopedic surgeons specializing in spinal diseases using chest high‐resolution computed tomography (CT) that was performed for the screening and prospecting of interstitial lung disease in 159 Japanese SSc patients. Among these patients, we identified 27 (17%) with spinal calcinosis, and the most common site was cervical level at 77.8% (21/27). The frequency of spinal calcinosis in the late stage was higher than in the early stage (44.4% vs 29.6%). Multiple calcinosis was identified in 18.5% (5/27). The frequency of paraspinal calcinosis only was 59.3%, intraspinal calcinosis only 18.5%, and both intraspinal and paraspinal calcinosis 22.2%. Among SSc patients, 4.4% (7/159) had CT‐based evidence of spinal cord compression. Among cases with spinal cord compression, only one had neurological symptoms, and surgical removal improved the symptoms. The other six SSc patients with spinal calcinosis (3.8% of 159) had no symptoms. Male sex (29.6%) and severe peripheral vasculopathy such as digital ulcers (55.6%) and acro‐osteolysis (33.3%) were significantly more frequent in the SSc patients with spinal calcinosis than in the SSc patients without spinal calcinosis (10.6%, 32.6% and 14.4%, respectively). Our results suggest that severe peripheral vasculopathy may be associated with the development of spinal calcinosis. Because SSc patients are prone to spinal calcinosis, when SSc patients claim symptoms such as pain, numbness and movement disorder of the extremities, spinal calcinosis is a complication that should be taken into consideration.
Disentangling the effect of drought on stand mortality and productivity in northern temperate and boreal forests
Authors: Laginha Pinto Correia David , Bouchard Mathieu , Filotas Élise , Raulier Frédéric 
Published: November 2018

Abstract

It has recently been reported that changing precipitation patterns increase tree mortality and reduce biomass accumulation in northern temperate and boreal forests. Functional diversity can mitigate the impacts of climate extremes in different types of ecosystems, but few studies have focused on tree functional diversity in forest ecosystems. It is critical to identify functional traits that could help anticipate the impact of drought on tree mortality, and how these affect above‐ground tree biomass productivity at the ecosystem level.

We tested how three functional traits (ratio of dry leaf mass per unit area [LMA], xylem pressure at which 50% of stem xylem conductivity is lost through cavitation [Ψ50] and leaf area to sapwood ratio) influence severe drought impacts on stand mortality and productivity. We used structural equation modelling to compare the effect of a latent variable composed of these three traits between plots that had suffered drought and plots that had not, on the mortality and productivity of northern temperate and boreal forests of Québec, Canada.

A latent variable composed of LMA and Ψ50 significantly explained drought‐induced tree mortality, but did not explain stand productivity response to severe drought. Therefore, even if these traits relate to the ability of species to survive drought (drought tolerance) at the tree level, they did not affect the maintenance of plant productivity during drought events (drought resistance) at the stand scale. We hypothesize that the effect of tree mortality on productivity was likely compensated by the formation of canopy openings that stimulate the growth of surviving trees.

Synthesis and applications. Our results show that mitigation of water loss and xylem resistance to cavitation contribute similarly to severe drought tolerance in trees within northern temperate and boreal forests. Therefore, including drought‐resistant trees in fine‐scale mixtures might mitigate the impacts of drought on forest productivity due to the survivors’ response to released resources.
Valuing height: diagnosis, valuation and the case of idiopathic short stature
Authors: Morrison Michael 
Published: November 2018

AbstractThis paper proposes a ‘valuographic’ approach to diagnosis, exploring how values and valuation practices are implicated in the contested diagnostic category of idiopathic short stature (ISS). ISS describes children who are ‘abnormally’ short but do not have any other detectable pathology. In the USA growth‐promoting hormone therapy has been approved for ISS children, since 2003. However, no other jurisdiction has approved this treatment and the value of ISS as a diagnostic category remains disputed among healthcare professionals. Drawing on qualitative interviews with paediatric endocrinologists in the UK and the US, this study presents a historical snapshot illustrating how the problematisation of ISS as a diagnosis involved multiple registers of value including epistemic, economic and moral calculations of worth. Contestation of the diagnosis was not just about what counts but about what ought to be counted, as respondents’ accounts of ISS gave differential weight to a range of types of evidence and methods of assessment. Ultimately what was at stake was not just the value of increased height for short patients, but what it meant to properly practice paediatric endocrinology. Consideration is then given to how a valuographic approach can be applied to sociological studies of diagnosis more broadly.