bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic succession of substrate-associated bacterial composition and function duringGanoderma lucidumgrowth
Authors: Zhang Bo , Yan Lijuan , Li Qiang , Zou Jie , Tan Hao , Tan Wei , Peng Weihong , Li Xiaolin , Zhang Xiaoping 
Published: June 2018

BackgroundGanoderma lucidum, a valuable medicinal fungus, is widely distributed in China. It grows alongside with a complex microbial ecosystem in the substrate. As sequencing technology advances, it is possible to reveal the composition and functions of substrate-associated bacterial communities.MethodsWe analyzed the bacterial community dynamics in the substrate during the four typical growth stages ofG. lucidumusing next-generation sequencing.ResultsThe physicochemical properties of the substrate (e.g. acidity, moisture, total nitrogen, total phosphorus and total potassium) changed between different growth stages. A total of 598,771 sequences from 12 samples were obtained and assigned to 22 bacterial phyla.ProteobacteriaandFirmicuteswere the dominant phyla. Bacterial community composition and diversity significantly differed between the elongation stage and the other three growth stages. LEfSe analysis revealed a large number of bacterial taxa (e.g.Bacteroidetes,AcidobacteriaandNitrospirae) with significantly higher abundance at the elongation stage. Functional pathway prediction uncovered significant abundance changes of a number of bacterial functional pathways between the elongation stage and other growth stages. At the elongation stage, the abundance of the environmental information processing pathway (mainly membrane transport) decreased, whereas that of the metabolism-related pathways increased.DiscussionThe changes in bacterial community composition, diversity and predicted functions were most likely related to the changes in the moisture and nutrient conditions in the substrate with the growth ofG. lucidum, particularly at the elongation stage. Our findings shed light on theG. lucidum-bacteria-substrate relationships, which should facilitate the industrial cultivation ofG. lucidum.
Boosting Antitumor Drug Efficacy with Chemically Engineered Multidomain Proteins
Authors: Kuan Seah Ling , Fischer Stephan , Hafner Susanne , Wang Tao , Syrovets Tatiana , Liu Weina , Tokura Yu , Ng David Yuen Wah , Riegger Andreas , Förtsch Christina , Jäger Daniela , Barth Thomas F. E. , Simmet Thomas , Barth Holger , Weil Tanja 
Published: June 2018

AbstractA facile chemical approach integrating supramolecular chemistry, site‐selective protein chemistry, and molecular biology is described to engineer synthetic multidomain protein therapeutics that sensitize cancer cells selectively to significantly enhance antitumor efficacy of existing chemotherapeutics. The desired bioactive entities are assembled via supramolecular interactions at the nanoscale into structurally ordered multiprotein complexes comprising a) multiple copies of the chemically modified cyclic peptide hormone somatostatin for selective targeting and internalization into human A549 lung cancer cells expressing SST‐2 receptors and b) a new cysteine mutant of the C3bot1 (C3) enzyme from Clostridium botulinum, a Rho protein inhibitor that affects and influences intracellular Rho‐mediated processes like endothelial cell migration and blood vessel formation. The multidomain protein complex, SST3‐Avi‐C3, retargets C3 enzyme into non‐small cell lung A549 cancer cells and exhibits exceptional tumor inhibition at a concentration ≈100‐fold lower than the clinically approved antibody bevacizumab (Avastin) in vivo. Notably, SST3‐Avi‐C3 increases tumor sensitivity to a conventional chemotherapeutic (doxorubicin) in vivo. These findings show that the integrated approach holds vast promise to expand the current repertoire of multidomain protein complexes and can pave the way to important new developments in the area of targeted and combination cancer therapy.
Versatile Core–Sheath Yarn for Sustainable Biomechanical Energy Harvesting and Real‐Time Human‐Interactive Sensing
Authors: Dong Kai , Deng Jianan , Ding Wenbo , Wang Aurelia C. , Wang Peihong , Cheng Chaoyu , Wang Yi‐Cheng , Jin Limin , Gu Bohong , Sun Baozhong , Wang Zhong Lin 
Published: June 2018

AbstractThe emergence of stretchable textile‐based mechanical energy harvester and self‐powered active sensor brings a new life for wearable functional electronics. However, single energy conversion mode and weak sensing capabilities have largely hindered their development. Here, in virtue of silver‐coated nylon yarn and silicone rubber elastomer, a highly stretchable yarn‐based triboelectric nanogenerator (TENG) with coaxial core–sheath and built‐in spring‐like spiral winding structures is designed for biomechanical energy harvesting and real‐time human‐interactive sensing. Based on the two advanced structural designs, the yarn‐based TENG can effectively harvest or respond rapidly to omnifarious external mechanical stimuli, such as compressing, stretching, bending, and twisting. With these excellent performances, the yarn‐based TENG can be used in a self‐counting skipping rope, a self‐powered gesture‐recognizing glove, and a real‐time golf scoring system. Furthermore, the yarn‐based TENG can also be woven into a large‐area energy‐harvesting fabric, which is capable of lighting up light emitting diodes (LEDs), charging a commercial capacitor, powering a smart watch, and integrating the four operational modes of TENGs together. This work provides a new direction for textile‐based multimode mechanical energy harvesters and highly sensitive self‐powered motion sensors with potential applications in sustainable power supplies, self‐powered wearable electronics, personalized motion/health monitoring, and real‐time human‐machine interactions.
Generalization and fine mapping of red blood cell trait genetic associations to multi‐ethnic populations: The PAGE study
Authors: Hodonsky Chani J. , Schurmann Claudia , Schick Ursula M. , Kocarnik Jonathan , Tao Ran , van Rooij Frank J. A. , Wassel Christina , Buyske Steve , Fornage Myriam , Hindorff Lucia A. , Floyd James S. , Ganesh Santhi K. , Lin Dan‐Yu , North Kari E. , Reiner Alex P. , Loos Ruth J. F. , Kooperberg Charles , Avery Christy L. 
Published: June 2018

AbstractRed blood cell (RBC) traits provide insight into a wide range of physiological states and exhibit moderate to high heritability, making them excellent candidates for genetic studies to inform underlying biologic mechanisms. Previous RBC trait genome‐wide association studies were performed primarily in European‐ or Asian‐ancestry populations, missing opportunities to inform understanding of RBC genetic architecture in diverse populations and reduce intervals surrounding putative functional SNPs through fine‐mapping. Here, we report the first fine‐mapping of 6 correlated (Pearson's r range: |0.04‐0.92|) RBC traits in up to 19 036 African Americans and 19 562 Hispanic/Latino participants of the Population Architecture using Genomics and Epidemiology consortium. Trans‐ethnic meta‐analysis of race/ethnic‐ and study‐specific estimates for approximately 11 000 SNPs flanking 13 previously identified association signals as well as 150 000 additional array‐wide SNPs was performed using inverse‐variance meta‐analysis after adjusting for study and clinical covariates. Approximately half of previously reported index SNP‐RBC trait associations generalized to the trans‐ethnic study population (p < 1.7 × 10−4); previously unreported independent association signals within the ABO region reinforce the potential for multiple functional variants affecting the same locus. Trans‐ethnic fine‐mapping did not reveal additional signals at the HFE locus independent of the known functional variants. Finally, we identified a potential novel association in the Hispanic/Latino study population at the HECTD4/RPL6 locus for RBC count (p = 1.9 × 10−7). The identification of a previously unknown association, generalization of a large proportion of known association signals, and refinement of known association signals all exemplify the benefits of genetic studies in diverse populations.
Twisted N‐Doped Nano‐Graphenes: Synthesis, Characterization, and Resolution
Authors: Hahn Uwe , Maisonhaute Emmanuel , Nierengarten Jean‐François 
Published: June 2018

AbstractTwo diastereoisomeric N‐doped nanographene derivatives have been efficiently prepared in two synthetic steps starting from an ethynylated hexaazatriphenylene building block. The first derivative adopts a D3‐symmetrical propeller‐shaped structure with three equivalent nanographene foils. The structure of the second diastereoisomer is C2‐symmetrical and differs from the first one by the way two peripheral nanographene foils overlap. Owing to their intertwined structures, the two N‐doped nanographenes are soluble in organic solvents and could be characterized by a combination of several analytical tools. Resolution of the D3‐symmetrical derivative has been achieved and CD measurements revealed extremely strong Cotton effects.
Enhanced mechanical flexibility and performance of sodium alginate polymer electrolyte bio‐membrane for application in direct methanol fuel cell
Authors: Shaari N. , Kamarudin S. K. , Basri S. , Shyuan L. K. , Masdar M. S. , Nordin D. 
Published: June 2018

ABSTRACTA new membrane was synthesized containing pure alginate, crosslinking agent (CaCl2), and plasticizer (glycerol). Characterization studies of the membrane were applied to determine the characteristics and morphology using field emission scanning electron microscope, EDX, FTIR, XRD, and atomic force microscopy analysis. The half‐cell performance test of the membrane was verified by several tests, including proton conductivity and methanol permeability. The best membrane had high proton conductivity (10.1 × 10−3 S cm−1) and very low methanol permeability (1.984 × 10−7 cm2 s−1), which consequently resulted in very high selectivity (5.0907 × 104 Ss cm−3). Glycerol had a positive modification and good influence on the alginate characteristics. Furthermore, the poor mechanical properties of the alginate biopolymer were enhanced by calcium chloride and glycerol inside the polymer. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46666.
Removal of copper ions from aqueous solutions by adsorption onto wheat straw cellulose‐based polymeric composites
Authors: Kong Wenjia , Li Qian , Li Xiaodi , Su Yuan , Yue Qinyan , Zhou Weizhi , Gao Baoyu 
Published: June 2018

ABSTRACTThe wheat straw cellulose‐based hydrogels were synthesized by graft copolymerization followed by semi‐interpenetrating network technology. The prepared hydrogels were characterized through various methods including Fourier transform infrared spectra, scanning electron microscope, thermogravimetric analysis, and X‐ray photoelectron spectroscopy. Batch adsorption experiments were carried out to investigate the adsorption performances of hydrogels toward Cu(II) ions. The results suggested that the introduction of semi‐interpenetrating network polymers, sodium alginate and poly(vinyl alcohol), could greatly enhance the adsorption property of hydrogels. And the wheat straw cellulose‐g‐poly(potassium acrylate)/sodium alginate hydrogel showed a highest Cu(II) ions adsorption capacity of 130 mg/g. The equilibrium isotherm and adsorption kinetics were also studied. Besides, the mass transfer coefficients and the thermodynamics of Cu(II) ions adsorption were also probed. Finally, the X‐ray photoelectron spectroscopy analysis further demonstrated that the Cu(II) ions adsorption was mainly via complexation reaction of NH2 and O‐containing groups in hydrogels. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46680.
Reduced graphene oxide–poly(methyl methacrylate) nanocomposite as a supercapacitor
Authors: Zabihi Zabiholah , Rodriguez Paul Eduardo David Soto , Boujakhrout Abderrahmane , Viera Juan Carlos , del Valle Jorge Alonso , Araghi Houshang , Villalonga Reynaldo 
Published: June 2018

ABSTRACTAn exhaustive study on a nanocomposite–poly(methyl methacrylate) matrix material as a supercapacitor is presented. The study includes morphological, structural, and electrochemical characterization. Different electrolytes were used, for which both pseudocapacitance (for reduced graphene oxide) and electric double‐layer capacitance (for functionalized graphene) were observed. An optimum nanocomposite weight percentage was found (2%), and the best performance with highest capacitance (30.4 F/g) was seen for the electrolyte including the smallest anions (OH−), being among the best values for similar systems, that is, a nanocomposite/nonconductive polymer matrix. In addition, a model is presented that explains the underlying electron transport mechanism. The results are promising for the construction of supercapacitors based on novel nanocomposite–poly(methyl methacrylate) matrix materials. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46685
Effect of ferric citrate on amyloid‐beta peptides behavior
Authors: Galante D. , Cavallo E. , Perico A. , D'Arrigo C. 
Published: June 2018

AbstractAmyloid beta (Aβ) aggregation and oxidative stress are two of the central events in Alzheimer's Disease (AD). Both these phenomena can be caused by the interaction of Aβ with metal ions. In the last years the interaction between ZnII, CuII, and Aβ was much studied, but between iron and Aβ it is still little known. In this work we determine how three Aβ peptides, present in AD, interact with FeIII‐citrate. The three Aβ peptides are: full length Aβ1‐42, an isoform truncated at Glutamic acid in position three, Aβ3‐42, and its pyroglutamated form AβpE3‐42. Conformation and morphology of the three peptides, aggregated with and without FeIII‐citrate were studied. Besides, we have determined the strength of the interactions Aβ/FeIII‐citrate studying the effect of ethylenediaminetetraacetic acid as chelator. Results reported here demonstrate that FeIII‐citrate promotes the aggregation in all the three peptides. Moreover, Aspartic acid 1, Glutamic acid 3, and Tyrosine 10 have an important role in the coordination with iron, generating a more stable complex for Aβ1‐42 compared to that for the truncated peptides.
Plasma cell‐free DNA quantification is highly correlated to tumor burden in children with neuroblastoma
Authors: Wang Xisi , Wang Lijun , Su Yan , Yue Zhixia , Xing Tianyu , Zhao Wen , Zhao Qian , Duan Chao , Huang Cheng , Zhang Dawei , Jin Mei , Cheng Xianfeng , Chen Shenglan , Liu Yi , Ma Xiaoli 
Published: June 2018

AbstractTo evaluate plasma cell‐free DNA (cfDNA) as a promising biomarker for neuroblastoma (NB) tumor burden. Seventy‐nine eligible patients with newly diagnosed NB were recruited from Beijing Children's Hospital between April 2016 and April 2017. Additionally, from September 2011 to June 2017, 79 patients with stable NB were evaluated with a median follow‐up time of 21 months. Approximately 2 mL of peripheral blood was drawn upon enrollment, and plasma cfDNA levels were measured via quantitative polymerase chain reaction (qPCR). Total cfDNA analysis was performed using the long interspersed nuclear element 1 (LINE‐1) 79 bp fragment, and DNA integrity was calculated by the ratio of the LINE‐1 300 bp fragment to the LINE‐1 79 bp fragment. A total of 79 NB patients with a median age of 36 months comprised the group of newly diagnosed NB patients. The main primary tumor site was the retroperitoneal and adrenal region (81%). Three or more metastatic sites were found in 17.7% of patients. Stable NB patients older than 18 months comprised 98.7% of the stable NB patients. Neuron‐specific enolase (NSE), lactate dehydrogenase (LDH), and cfDNA levels were dramatically increased in the newly diagnosed NB patients and significantly different from those in the stable NB patients. Moreover, the concentration of cfDNA was much higher in patients with larger tumors. By analyzing the area under the receiver operator characteristic (ROC) curve (AUC), the areas of total cfDNA, NSE, and LDH levels were 0.953, 0.929, and 0.906, respectively. The sensitivity and specificity data clarified that the level of circulating cfDNA in plasma can be considered as a reliable biomarker for describing tumor load in NB. The plasma cfDNA concentration was as good as the levels of LDH and NSE to discriminate the tumor burden in children with NB.
Feasibility of using negative ultrasonography results of axillary lymph nodes to predict sentinel lymph node metastasis in breast cancer patients
Authors: Chen Xue , He Yingjian , Wang Jiwei , Huo Ling , Fan Zhaoqing , Li Jinfeng , Xie Yuntao , Wang Tianfeng , Ouyang Tao 
Published: June 2018

AbstractKnowledge of the pathology of axillary lymph nodes (ALN) in breast cancer patients is critical for determining their treatment. Ultrasound is the best noninvasive evaluation for the ALN status. However, the correlation between negative ultrasound results and the sentinel lymph nodes (SLN) pathology remains unknown. To test the hypothesis that negative ultrasound results of ALN predict the negative pathology results of SLN in breast cancer patients, we assessed the association between ALN ultrasonography‐negative results and the SLN pathology in 3115 patients with breast cancer recruited between October 2010 and April 2016 from a single cancer center, prospective database. Of these patients who met the inclusion criteria, 2317 (74.4%) had no SLN pathological metastasis. In the univariate analysis, other 798 patient with positive SLN tended to be under age 40 and premenopausal, having large tumor sizes (>2 cm), higher histological grade of primary tumor, positive hormone receptors, and negative HER‐2 status (P < .05 for all). In the multivariate analysis, menstrual status, tumor size, ER status and histological types of primary tumor remained to be independent predictors for SLN pathological metastasis. The area under curve (AUC) was 0.658 (95% CI = 0.637‐0.679), P > .05. In conclusion, only a 74.4% consistency between ALN ultrasonography‐negative results and negative pathological SLN results, although menstrual status, tumor size, histologic subtypes of primary tumor and ER status were found to be statistically independent predictors of positive SLN among patients negative for ALN ultrasonography. Therefore, the present study suggests that negative ultrasound results of ALN do not adequately predict the negative pathology results of SLN in breast cancer patients.
Adjuvant radiotherapy after curative surgery for oral cavity squamous cell carcinoma and treatment effect of timing and duration on outcome—A Taiwan Cancer Registry national database analysis
Authors: Cheng Yung‐Jen , Tsai Mu‐Hung , Chiang Chun‐Ju , Tsai Sen‐Tien , Liu Tsang‐Wu , Lou Pei‐Jen , Liao Chun‐Ta , Lin Jin‐Ching , Chang Joseph Tung‐Chieh , Tsai Ming‐Hsui , Chu Pen‐Yuan , Leu Yi‐Shing , Tsai Kuo‐Yang , Terng Shyuang‐Der , Chien Chih‐Yen , Yang Muh‐Hwa , Hao Sheng‐Po , Wang Chuan‐Cheng , Tsai Ming‐Hsun , Chen Helen H. W. , Kuo Chin , Wu Yuan‐Hua 
Published: June 2018

AbstractConduct an accurate risk assessment of resected oral cavity squamous cell carcinoma (OSCC) patients by accessing a nationwide systemic investigation is pivotal to improve treatment outcomes. In this article, we tried to determine the impact of different prognostic factors for OSCC patients who received adjuvant radiotherapy (RT) after curative surgery, using Taiwan's national cancer registry database (TCR). A nationwide, large population‐based study was conducted using TCR with patients identified from 2007 to 2015. The study variables included age, gender, cancer subsites, stage, histology grade, margin and extra‐nodal extension (ENE) status, treatment type, surgery to RT interval (ORI), total RT treatment time (RTT), and RT dose. Univariate and multivariate analysis were performed to identify predictors of the variables associated with overall survival (OS), cause‐specific survival (CSS), local‐regional relapse‐free survival (LRFS), and distant metastasis‐free survival (DMFS). 8986 OSCC patients treated with surgery and adjuvant RT were analyzed. In multivariate analysis, worse outcomes were associated with males, older age, subsite in the oral tongue, advanced stage, higher histologic grade, involved margin, and positive ENE. ORI only showed an adverse trend in LRFS, when exceeding 7 weeks (P = .06). RTT >8 weeks was a significant poor predictor in OS, CSS and LRFS (P < .001). Extreme RT dose (>70 Gy or ≤50 Gy) also demonstrated an adverse impact on the outcomes. Prolonged RT treatment time and extreme RT doses were identified as significantly poor prognostic predictors in OSCC patients who received adjuvant RT after curative surgery.
Down‐regulation of exosomal miR‐106b‐5p derived from cholesteatoma perimatrix fibroblasts promotes angiogenesis in endothelial cells by overexpression of Angiopoietin 2
Authors: Li Yang , Liang JianMin , Hu Juan , Ren Xiaoyong , Sheng Ying 
Published: June 2018

AbstractHuman cholesteatoma perimatrix fibroblasts (hCPFs) can stimulate the endothelial cells of nearby microvessels to proliferate and migrate in a paracrine manner. Exosomes, secreted from various cell types, are one of the most important paracrine factors and play critical roles in intercellular communication. However, whether exosomes derived from human cholesteatoma perimatrix fibroblasts (hCPFs‐Exo) can promote angiogenesis has not been reported. In this study, we isolated exosomes secreted by hCPFs and observed that hCPFs‐Exo was able to promote migration and tube formation in human umbilical vein endothelial cells (HUVECs). Advanced studies revealed hCPFs‐Exo with low expression of miR‐106b‐5p was transferred into HUVECs, and decreased expression of miR‐106b‐5p could promote angiogenesis by targeting Angiopoietin 2 (Angpt2) via binding to its 3′‐UTR. Furthermore, low levels of miR‐106b‐5p triggered overexpression of Angpt2, and significantly increased HUVEC migration and tube formation. Taken together, our results suggest that hCPFs‐Exo transports low expressed exosomal miR‐106b‐5p to endothelial cells and promotes angiogenesis by overexpression of Angpt2.
Gynecomastia after euthyroidism restoration in a patient with type 1 diabetes and Graves’ disease
Authors: Calcaterra Valeria , Clerici Edoardo , Ceolin Valeria , Regalbuto Corrado , Larizza Daniela 
Published: June 2018

Key Clinical MessageIn patients with autoimmune disease, gynecomastia should not be considered as 1 of the first signs of hyperthyroidism, rather it is a breast pathology that can be present even when euthyroidism restoration is achieved. It is unknown whether the autoimmune nature of thyroid disorders or simply the hyperthyroidism effects breast changes.
Size‐Dependent Visible Light Photocatalytic Performance of Cu2O Nanocubes
Authors: Karthikeyan Sekar , Kumar Santosh , Durndell Lee J. , Isaacs Mark A. , Parlett Christopher M. A. , Coulson Ben , Douthwaite Richard E. , Jiang Zhi , Wilson Karen , Lee Adam F. 
Published: June 2018

AbstractWell‐defined Cu2O nanocubes with tunable dimensions and physicochemical properties have been prepared using a simple one‐pot reaction. Reduction of Cu(II) salts by ascorbic acid in the presence of PEG as a structure‐directing agent affords crystalline Cu2O nanocubes of between 50 to 500 nm. Optical band gap, band energies, charge‐carrier lifetimes and surface oxidation state systematically evolve with nanocube size, and correlate well with visible light photocatalytic activity for aqueous phase phenol degradation and H2 production which are both directly proportional to size (doubling between 50 and 500 nm). HPLC reveals fumaric acid as the primary organic product of phenol degradation, and selectivity increases with nanocube size at the expense of toxic catechol. Apparent quantum efficiencies reach 26 % for phenol photodegradation and 1.2 % for H2 production using 500 nm Cu2O cubes.