bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coculturing of mesenchymal stem cells of different sources improved regenerative capability of osteochondral defect in the mature rabbit: An in vivo study
Authors: Mahmoud Elhussein Elbadry , Adachi Nobuo , Mawas Amany Sayed , Gaarour Osama Samir , Ochi Mitsuo 
Published: April 2019

Purpose: Choosing a therapeutic cell source for osteochondral repair remains a challenge. The present study investigated coculturing mesenchymal stem cells (MSCs) from different sources to provide an improved therapeutic cell option for osteochondral repair. Methods: Dutch and Japanese white rabbits were used in this study, the first for isolating MSCs and the second for creating an osteochondral model in the medial femoral condyle. The 26 rabbit knees were divided randomly into four groups: control ( n = 6), bone marrow-derived MSCs (BMSCs) ( n = 7), synovial tissue MSCs (SMSCs) ( n = 7), and cocultured MSCs ( n = 6). Tissue repair was assessed using the Fortier scale, and colony-forming assay was performed. Results: At different cell densities, cocultured and SMSCs formed larger colonies than BMSCs, indicating their high proliferative potential. After 2 months, complete filling of the defect with smooth surface regularity was detected in the cocultured MSC group, although there was no significant difference among the therapeutic groups macroscopically. Also, tissue repair was histologically better in the cocultured MSC group than in the control and SMSC groups, due to repair of the subchondral bone and coverage with hyaline cartilage. Additionally, toluidine blue and collagen-II staining intensity in the repaired tissue was better in the cocultured MSC group than in the remaining groups. Conclusion: Our results suggest that cocultured MSCs are a suitable option for the regeneration capability of osteochondral defects due to their enhanced osteochondrogenic potential.
Eight-year results of transtibial nonanatomic single-bundle versus double-bundle anterior cruciate ligament reconstruction: Clinical, radiologic outcomes and survivorship
Authors: Yoon Kyoung Ho , Kim Jung Suk , Kim Sang Jun , Park Moonsu , Park Soo Yeon , Park Sang Eon 
Published: April 2019

Purpose: To compare the long term outcomes of transtibial nonanatomic single-bundle (SB) and double-bundle (DB) anterior cruciate ligament (ACL) reconstruction. Methods: Between January 2008 and September 2010, we retrospectively evaluated 377 patients who underwent primary ACL reconstruction and who were available at 8 years of follow-up. Patients who received transtibial nonanatomic SB ACL reconstruction ( n = 263) were assigned to group SB and DB ACL reconstruction ( n = 114) to group DB. The patients were assessed with the International Knee Documentation Committee (IKDC), the Lysholm scores, Tegner activity score, knee joint stability tests, and patellofemoral osteoarthritis (OA) findings using the Kellgren–Lawrence (K-L) classification. Contralateral ACL (CACL) injury was also evaluated. Results: Thirteen patients underwent revision ACL surgery during the follow-up. Ultimately, 256 patients in group SB and 108 patients in group DB were available at the 8-year follow-up. At final follow-up, the IKDC subjective score ( p = 0.04) and Lysholm score ( p = 0.02) showed significantly superior results in group DB compared to group SB. However, there was no significant difference of Tegner activity score ( p = 0.30), range of motion ( p = 0.81), and knee joint stability tests. There was significant progression of patellofemoral OA between preoperative and final follow-up in ipsilateral knee of both groups but not significant progression in contralateral knee. However, there was no significant difference between the two groups at the follow-up. There were 2.7% ipsilateral ACL graft failure in group SB, compared with 5.2% in group DB ( p = 0.12) and 6.4% CACL tear in group SB, compared with 5.2% in group DB ( p = 0.65). The mean survivorship of ACL graft was 9.47 ± 0.05 years (95% confidence interval (CI), 9.36–9.58) in group SB and 9.87 ± 0.16 years (95% CI, 9.54–10.20) in group DB, and there was no significant difference ( p = 0.25). Conclusion: DB ACL reconstruction resulted in significantly better clinical IKDC subjective scores and Lysholm scores. However, most of the differences in clinical scores were small, and the clinical relevance of this difference is unknown. In addition, there was no significant difference in Tegner activity score, knee joint stability tests, patellofemoral OA, and survivor rate of the ACL graft and CACL after reconstruction after a minimum of 8-year follow-up. Level of Evidence: Cohort study; level of evidence, 3.
A Comparison of Team Sport Volume Surveyed Between High School and Club Sport Coaches
Authors: Post Eric G. , Schaefer Daniel A. , Biese Kevin M. , Watson Andrew M. , McGuine Timothy A. , Brooks M. Alison , Bell David R. 
Published: April 2019

Background: Club sport participation is increasingly common among high school athletes. Sport participation characteristics may vary widely between the high school and club sport settings. However, there have been no large-scale comparisons of sport participation volume between high school and club teams from similar sports. Purpose/Hypothesis: The purpose of this study was to compare the sport participation characteristics of high school and high school–aged club teams. We hypothesized that club team athletes would participate in more months per year and hours per week and would take more overnight trips than high school athletes and that club team coaches would be less likely to track their athletes’ sport volume than high school coaches. Study Design: Cross-sectional study. Methods: A total of 769 coaches (266 female; 34.6%) from 3 sports (basketball, volleyball, soccer) completed an anonymous online questionnaire regarding their team’s sport volume and their knowledge, attitudes, and beliefs regarding sport specialization. Coaches were eligible to participate if they had served as the head or assistant coach of a high school sport team or high school–aged club sport team in the past 12 months. Results: Overall, 64.6% (n = 497) were coaches of a high school team, and 35.4% (n = 272) were coaches of a club team with high school–aged athletes. Club coaches’ teams played more months out of the year and took more overnight trips for competitions than high school coaches’ teams but participated in fewer hours per week of practices or competitions during their season. There were no differences between club and high school coaches in tracking their athletes’ sport volume, with most coaches in both settings reporting that they do not track the amount of time that their athletes participate in other sports. Conclusion: Significant differences in sport participation volume exist between the teams of high school and club coaches.
Targeting CD47 in Sézary syndrome with SIRPαFc
Authors: Johnson Lisa D. S. , Banerjee Swati , Kruglov Oleg , Viller Natasja Nielsen , Horwitz Steven M. , Lesokhin Alexander , Zain Jasmine , Querfeld Christiane , Chen Robert , Okada Craig , Sawas Ahmed , O’Connor Owen A. , Sievers Eric L. , Shou Yaping , Uger Robert A. , Wong Mark , Akilov Oleg E. 
Published: April 2019

AbstractSézary syndrome (SS), the leukemic variant of cutaneous T-cell lymphoma, has limited treatment options and rare occurrences of long-term remission, thus warranting research into new treatment approaches. CD47 has emerged as a promising target for multiple tumor types, but its role in SS remains unknown. Here, we show that CD47 is highly expressed on Sézary cells in the peripheral blood and skin, and the high level of CD47 expression correlates with worse overall survival (OS) in patients with SS. We also demonstrate that CD47 expression on Sézary cells is under the influence of interleukin 4 (IL-4), IL-7, and IL-13. Signal regulatory protein αFc (SIRPαFc; TTI-621), a novel CD47 decoy receptor, triggers macrophage-mediated phagocytosis of Sézary cells and, when administered in clinical trial settings, results in significant tumor load reduction. We conclude that inhibition of the CD47-SIRPα signaling pathway has therapeutic benefit for patients with SS. This trial was registered at www.clinicaltrials.gov as #NCT02663518.
Nebulised lipid–polymer hybrid nanoparticles for the delivery of a therapeutic anti-inflammatory microRNA to bronchial epithelial cells
Authors: Vencken Sebastian , Foged Camilla , Ramsey Joanne M. , Sweeney Louise , Cryan Sally-Ann , MacLoughlin Ronan J. , Greene Catherine M. 
Published: April 2019

Modulation of microRNAs (miRNAs), endogenous regulators of gene expression, is a promising strategy for tackling inflammatory lung diseases. In this proof-of-concept study, we tested delivery of miR-17 to bronchial epithelial cells (BECs) using nebulised lipid–polymer hybrid nanoparticles (LPNs). The primary aim was to reduce the induced secretion of miR-17's target,i.e.the pro-inflammatory chemokine interleukin (IL)-8.Synthetic miR-17 mimics were loaded into LPNs composed of poly(dl-lactic-co-glycolic acid) (PLGA) and the cationic lipid 1,2-dioleoyloxy-3-(trimethylammonium)propane (DOTAP) using a double emulsion solvent evaporation method and nebulised using the Aerogen Solo nebuliser. The physicochemical, aerosol, inflammatory and cytotoxic properties of LPNs were characterised. The effect of LPNs on lipopolysaccharide (LPS)-induced IL-8 production from human NuLi-1 BECs was tested by ELISA.Thez-average, polydispersity index and ζ-potential of the LPNs and the aerodynamic properties of nebulised suspensions were in a range optimal for deposition in the bronchi and bronchioles post-inhalation. Cytotoxic and pro-inflammatory effects were minimal for LPNs loaded with a model cargo. Nebulisation did not affect the physicochemical or functional properties of the LPNs. Nebulised miR-17-loaded LPNs downregulated LPS-induced IL-8 secretion by >40% in BECs.This study suggests that DOTAP-modified PLGA LPNs are efficient and well-tolerated carriers for delivery of miRNA mimics to BECs.
Sclerostin antibodies as novel anabolic therapy for osteoporosis
Authors: Mamedova Elizaveta O. , Grebennikova Tatiana A. , Belaya Zhanna E. , Rozhinskaya Liudmila Y. 
Published: April 2019

Osteoporosis medications are divided into two groups: those inhibiting bone resorption and formation (bisphosphonates and denosumab), and those stimulating bone formation i.e. having an anabolic effect. The latter include teriparatide, parathyroid hormone 1-84 and abaloparatide, all of which stimulate bone resorption as well as bone formation, which limits their anabolic effect.
The discovery of sclerostin – the key inhibitor of bone formation – has led to development of the concept that inhibition of this protein could stimulate bone formation. Romosozumab is a human monoclonal antibody to sclerostin that binds to sclerostin and enables Wnt-signaling pathway ligands and their co-receptors to interact with each other, which, in turn, leads to increased bone formation and bone mineral density. Unlike classical anabolic drugs in osteoporosis treatment, romosozumab stimulates bone formation and inhibits bone resorption. In clinical trials, romosozumab showed marked increase in lumbar spine and hip bone mineral density. Presented article contains information about pre-clinical and clinical studies of romosozumab.
Térdközeli lateralis cystosus elváltozások differenciáldiagnosztikája
Authors: Szuper Kinga , Giyab Omar , Than Péter 
Published: April 2019

Abstract: Soft tissues detected on the lateral side of the knee joint may have differential diagnostic difficulties. Although most of these are benign, mostly cystic in appearance, they may raise the clinical suspicion of tumors due to their increased growth. The authors describe two rare soft tissue changes in three patients. They review the diagnostic procedures most commonly used in daily care, present surgical options, and discuss the literature of the subject. Orv Hetil. 2019; 160(15): 593–599.
Forma kanoniczna zawierania małżeństwa – spojrzenie historyczne i postulat reformy
Authors: Dohnalik Jan 
Published: April 2019

Kanoniczna forma zawarcia małżeństwa, wymagana do jego ważności, została wprowadzona przez dekret Tametsi Soboru Trydenckiego (1563). Z uwagi na fakt, że ta ważna norma prawa kanonicznego nigdy nie obowiązywała w całym Kościele, na początku XX wieku miała miejsce dyskusja odnośnie do nowych rozwiązań. To nowe prawo, zwane Ne temere, zostało ogłoszone w 1907 roku na polecenie papieża Piusa X, jako dekret Kongregacji Soboru. Chociaż było to prawo całościowe i dobrze przemyślane, podczas Soboru Watykańskiego II i późniejszej rewizji Kodeksu prawa kanonicznego były zaproponowane liczne projekty zmian. Ostatecznie obowiązujące prawo zasadniczo utrzymało zasadę kompetencji terytorialnej asystującego duchownego. Obecnie ma miejsce dyskusja w literaturze kanonistycznej odnośnie do potrzeby zreformowania kanonicznej formy małżeństwa. W tym kontekście po przeanalizowaniu dwudziestowiecznych dyskusji autor wspiera jedno z zaproponowanych rozwiązań. Podczas gdy utrzymuje się wymóg formy kanonicznej do ważności małżeństwa, należałoby wprowadzić możliwość zawierania małżeństwa przed jakimkolwiek wyświęconym kapłanem. To rozwiązanie byłoby zrozumiałe dla wiernych i gwarantowałoby sakramentalny charakter małżeństwa. Propozycja współgra z innymi normami prawnymi dotyczącymi sakramentów, łącznie z chrztem. Wreszcie postulowana reforma pomogłaby uniknąć nieważnych małżeństw spowodowanych zaniedbaniem lub niewiedzą duchownych.
Rady ewangeliczne w życiu dziewicy konsekrowanej
Authors: Stypułkowska Beata 
Published: April 2019

W artykule poruszono zagadnienie dziewic konsekrowanych jako indywidualnej formy życia konsekrowanego. Na samym początku poruszono kwestię określenia osób konsekrowanych i profesji rad ewangelicznych jako cechy ich charakteryzującej. Następnie uwaga została skoncentrowana na specyfice dziewic konsekrowanych, które składają tylko jeden ślub – ślub czystości. Najpierw zostały podane pewne uwagi dotyczące tego ślubu. W dalszej kolejności była mowa o dwóch radach ewangelicznych: ubóstwie i posłuszeństwie realizowanych w konkretach Kościoła diecezjalnego. Ubóstwo zostało powiązane z dobrami Kościoła i piątym przykazaniem kościelnym, natomiast posłuszeństwo, które odnosi się do Chrystusa, zostało powiązane z hierarchicznym ustrojem Kościoła oraz przykazaniem miłości i było rozpatrywane jako posłuszeństwo słowu Bożemu i przełożonym w Kościele. Na samym końcu zostało poruszone zagadnienie wolności i dobrowolności w stosowaniu rad ewangelicznych.
Czynności administracyjne i cywilnoprawne poprzedzające zawarcie małżeństwa konkordatowego przez pełnomocnika w Polsce
Authors: Świto Lucjan , Tomkiewicz Małgorzata 
Published: April 2019

Gdy w grę wchodzi zawarcie małżeństwa konkordatowego per procura, czynności administracyjne i cywilnoprawne poprzedzające zawarcie małżeństwa w formie wyznaniowej ze skutkami cywilnymi w prawie polskim ujawniają istotne wątpliwości. Dotyczą one wszystkich podmiotów uczestniczących w tym postępowaniu, to jest obowiązków nupturientów, pełnomocnika, kierownika USC, a także duchownego. Wątpliwości istnieją także co do zakresu kognicji sądu prowadzącego postępowanie w przedmiocie udzielenia zgody na zawarcie małżeństwa przez pełnomocnika.Artykuł poprzez analizę obowiązujących uregulowań polskiego kodeksu prawa rodzinnego, kodeksu postępowania cywilnego, ustawy Prawo o aktach stanu cywilnego oraz prawa wewnętrznego Kościoła katolickiego zawiera próbę odpowiedzi na te pytania.
The Role of Urban Pattern Indicators in Achieving Sustainable Urban Forms in the Developed Countries - A Pragmatic Evaluation of Two Sustainable Urban Contexts
Authors: Ibrahim Dr. Rebwar , Mushatat Prof. Sabah , Abdelmonem Prof. Mohammed 
Published: April 2019

Since cities are constantly changing, their new forms and activities are developed according to their patterns and physical arrangements. Sustainable urban forms are currently a widely discussed topic in the concept of urban sustainability. The physical forms of cities can be conceptualised as two interlocking planes. The first comprises the spatial distribution of activities and buildings. The second is made up of the pattern of streets, parks and the public realm. Sustainable integration between these layers leads to a compatibly built environment. This paper intends to formulate sustainable urban form indicators as a conceptual framework model that provides a comprehensive understanding of how sustainability can be evaluated and measured according to the pattern of any built environment. This framework consists of eight indicators: Accessibility, Connectivity, Compatibility, Diversity, Nodality, Density, Identity, and Adaptability. Accordingly, two of the most significant sustainable urban districts have been chosen for field study. The first is the Hammarby residential district in Stockholm, Sweden and the second is the Vauban residential district in Freiburg, Germany. The research concludes that good performance of urban pattern indicators in these developed communities may motivate, inform and improve design strategies for achieving a more sustainable urban context.
Bridging Body and Mind: Case Series of a 10-Week Trauma-Informed Yoga Protocol for Veterans
Authors: Justice Lauren , Brems Christiane 
Published: April 2019

Abstract
This case series explored the feasibility and preliminary efficacy of therapeutic yoga as a complementary form of treatment for combat-related trauma. The series recruited for and implemented a 10-week Trauma-Informed Yoga protocol for veterans in an interprofessional community health treatment setting. Participants were enrolled in a series of 90-minute therapeutic yoga classes adapted to be trauma-informed. Feasibility was measured by recruitment, retention, and level of participation in the study. Preliminary efficacy was explored via the Posttraumatic Stress Disorder Checklist, Scale of Body Connection, PROMIS-29, PROMIS Alcohol Use, PROMIS Substance Use, Difficulties in Emotional Regulation Scale, and Self-Compassion Scale–Short Form. All measures were administered at baseline, week 5, week 10, and at a 5-week follow-up. A qualitative Feasibility Questionnaire was administered weekly and at the 5-week follow-up to assess barriers and motivators for home practice and to collect feedback about session content. Recruitment challenges resulted in only seven interested individuals. Four participants (three males, one female) were successfully enrolled in the study after seven phone screenings and five in-person interviews. The four enrolled clients had a 100% follow-up retention rate, reported no adverse events, and on average participated in 85% of classes. Clinically significant enhancements were observed on trauma- and body connection–related scales for three participants from baseline to follow-up. Qualitative data revealed that motivators to practice include in-session philosophical discussions based on psychological themes; breathwork; mindfulness; and physical, social, work/academic, and mental health impact. Barriers included motivation, time, and location. Important themes emerged related to cultural considerations for veterans. Although this 10-week trauma-informed protocol faced challenges to recruitment, retention and participation were high. Efficacy measures yielded promising results for reducing trauma-related symptoms.
Body fat mass in patients with chronic obstructive pulmonary disease as a risk factor for right ventricular structural-functional changes
Authors: Kostareva R. A. , Golovskoy B. V. , Podyanova A. I. , Gerasimova A. V. 
Published: April 2019

Aim. To study the correlation between the body fat mass (BFM) and the structural-functional characteristics of right ventricle (RV) in patients with different body mass, suffering from chronic obstructive pulmonary disease (COPD).
Materials and methods. Echocardiography, ultrasound determination of subcutaneous and premesenterial layer thickness, assessment of leptin level was performed in 72 COPD patients, who were divided into three groups: group 1 – patients with normal body mass (n = 31), group 2 – patients with excess weight (n = 21), group 3 – patients with class I-II obesity (n= 20).
Results. Among COPD patients, body mass index (BMI) growth causes increase in BFM and subcutaneous and premesenterial fat thickness, elevation in blood serum leptin level, but decrease in lean body mass (LBM). The global longitudinal systolic strain of the free wall of RV in COPD patients with obesity (–17.39 ± 3.64, p = 0.002) and elevated body mass (–17.96 ± 5.69, p = 0.006) is reduced.
Conclusions. Among COPD patients with different body mass, the global longitudinal systolic strain of RV free wall aggravates as leptin level and body fat mass grow that can be the reflection of mechanical exposure and epicardial fat pressure on the right ventricle.
Lower tourniquet cuff pressure reduces postoperative thigh pain in obese patients undergoing total knee arthroplasty
Authors: Png Wenxian , Lee Wuchean , Tan Mann Hong 
Published: April 2019

<p class="abstract"><strong>Background:</strong> The use of high tourniquet pressures in obese patients undergoing total knee arthroplasty increases the risk of postoperative thromboembolic events and skin complications. Measurement of limb occlusion pressure (LOP) before surgery may lead to the use of lower tourniquet cuff pressure during surgery and thereby reduce the risk of postoperative pain and complications.</p><p class="abstract"><strong>Methods:</strong> Eighty-six obese patients scheduled for total knee arthroplasty were randomized to a control group (n=43) with tourniquet pressures at 300 mmHg or the LOP group (n=43) where a recommended tourniquet pressure was determined based on the patient’s limb occlusion pressure. The primary outcome measure was postoperative thigh pain; the secondary outcome measures were the quality of bloodless field, postoperative drop in haemoglobin, postoperative complications and Oxford knee scores.<strong></strong></p><p class="abstract"><strong>Results:</strong> The tourniquet cuff pressure was significantly lower in the LOP group than in the control group (p&lt;0.001). Patients in the LOP group showed significantly lower postoperative thigh pain scores till postoperative day 3. Our study did not show any difference in intraoperative quality of bloodless field (p&lt;0.103), postoperative complications and Oxford knee scores (p&lt;0.775) at six months after surgery.</p><p><strong>Conclusions:</strong> Our results show that the use of limb occlusion pressure method results in decreased postoperative thigh pain without reducing the quality of the bloodless field. We believe that this method in tourniquet application is safe and beneficial for the subset of obese patients undergoing total knee arthroplasty. </p>
Mengapa Kepercayaan Diri Mempengaruhi Kemampuan Menulis Kreatif Siswa?
Authors: Gunawan Himan Muttaqin , Aziz Rahmat 
Published: April 2019

Penelitian ini bertujuan untuk menguji hubungan antara kepercayaan diri dengan kemampuan menulis kreatif siswa. Jumlah sampel pada penelitian ini berjumlah 73 siswa Madrasah Aliiyah Negeri 2 Bogor. Metode pengumpulan data adalah skala kepercayaan diri, sementara metode pengumpulan data yang digunakan untuk variabel kemampuan menulis kreatif menggunakan tes menulis kreatif. Hasil analisis menunjukan adanya hubungan positif antara kepercayaan diri dengan kemampuan menulis kreatif, semakin tinggi tingkat kepercayaan diri maka semakin tinggi pula tingkat kemampuan menulis kreatif. Variabel kepercayaan diri mampu menjadi prediktor bagi tinggi rendahnya kemampuan menulis kreatif sebesar 6,1%.