bluuebunny/crossref_metadata_2025_split huggingface dataset:
The potential for civic engagement of older persons in the ageing society of Thailand
Authors: Tangchonlatip Kanchana , Chamratrithirong Aphichat , Lucktong Aksarapak 
Published: July 2019

Purpose
Several studies revealed the importance of older persons’ contribution to society. The enhancement of their potential to engage in civic activities should be encouraged. The purpose of this paper is to investigate factors associated with their civic engagement potential.

Design/methodology/approach
Data are from Thailand’s National Survey of Older Persons conducted by the National Statistical Office in 2011. A sample of 24,433 persons aged 60 years or older was analyzed to assess their engagement in four community activities. The data were subjected to logistic regression analysis.

Findings
This study found that the readiness and willingness to engage in socially productive activities of Thai older persons ranged from 7 to 23 percent depending on type of activities. The factors of ageing health, confidence in their family’s ability to assist them, and satisfaction with government services were significantly associated with their readiness and willingness to engage in socially productive activities. Inadequacy of income was not found to be an obstructing factor toward their readiness and willingness to engage in civic life in general, it inhibited only the sharing of knowledge and skills with others.

Originality/value
The findings reflect the impact of internal constraining factors and external enhancing factors on engagement of Thai older persons in civic activities. Policy implications on the role of the government to promote active ageing are discussed.
Halal certification or ingredient disclosure
Authors: Takeshita Shuko 
Published: July 2019

Purpose
The purpose of this study is to clarify what is really needed to attract Muslim tourists by comparing the two main approaches to serving food with halal certification or with ingredient disclosure.

Design/methodology/approach
This research adopted a comparative analysis of a Group promoting “halal certification” and a Group promoting “ingredient disclosure” mainly from the tourists’ perspective. Taito Ward, Tokyo, encourages restaurants to obtain halal certification, while the approach of Takayama City is to disclose the ingredients. The study compares the advantages and disadvantages of these two approaches and also investigates factors influencing them.

Findings
This study revealed that Muslim tourists would like to see both halal certification and ingredient disclosure. The reason why Taito Ward and Takayama City pursue different strategies can be summarized in three points: the types of food offered in the two areas, the vastly different scales of the two areas as tourist destinations and the different perspectives of their respective advisors.

Research limitations/implications
The limitations of this study include the small sample size and the low diversity of the origins of Muslim tourists. However, this research is still significant because few academic studies on halal certification have been done from the tourists’ perspective.

Originality/value
There are many things that could be done to attract Muslim tourists. Even without halal certification, there are many restaurants that can cater to Muslims in Japan. A little consideration and thoughtfulness on the part of restaurants can enhance the probability of attracting Muslim tourists.
A Fatal Case of Poisoning with Fentanyl Transdermal Patches in Japan
Authors: Nara Akina , Yamada Chiho , Saka Kanju , Kodama Takanori , Yoshida Masaki , Iwahara Kaori , Takagi Tetsuya 
Published: July 2019

AbstractFentanyl transdermal patches have been used to treat cancer‐ and noncancer‐related chronic pain. However, its inappropriate or illegal application may cause fatal poisoning. We herein present the case of a Japanese woman in her 40s who was found dead with seven 25‐μg/h fentanyl transdermal patches on her body. We established a detailed toxicological analysis procedure to quantify fentanyl, and its metabolite norfentanyl, and other drugs (acetaminophen, allylisopropylacetylurea, celecoxib, estazolam, promethazine, and sertraline) in human whole blood by ultra‐high‐performance liquid chromatography–tandem mass spectrometry. The measured fentanyl and norfentanyl concentrations in the femoral and cardiac blood were 0.051 and 0.072 μg/mL and 0.033 and 0.076 μg/mL, respectively. The decedent's fentanyl concentrations were consistent with previously reported postmortem blood levels for fatal cases of poisoning by fentanyl transdermal patches. Based on the decedent's case history, autopsy findings, and toxicological analyses, the cause of death was identified as intoxication with transdermal fentanyl.
Causal effect of alcohol consumption on hyperuricemia using a Mendelian randomization design
Authors: Jee Yon Ho , Jung Keum Ji , Park Yong‐Beom , Spiller Wes , Jee Sun Ha 
Published: July 2019

AbstractAimWe used a Mendelian randomization analysis to assess the causal effect of alcohol consumption on hyperuricemia in Koreans.MethodsThe Korean Cancer Prevention Study‐II (KCPS‐II) Biobank cohort consisted of 156 701 healthy Korean aged 20 years or older. Clinical data including serum uric acid, alcohol consumption, and other related confounding variables were collected at baseline. The 27 single nucleotide polymorphisms (SNP) including rs671 in aldehyde dehydrogenase 2 (ALDH2) were obtained from a genome‐wide association study of alcohol consumption in the KCPS‐II Biobank among 11 678 men and women in 2017. Both unweighted and weighted genetic risk score (wGRS) were calculated using 10 SNPs selected based on linkage disequilibrium.ResultsAs strong instrumental variables, both rs671 and wGRS were associated with an increased amount of alcohol drinking in men and women. Alcohol consumption was also positively associated with hyperuricemia risk in men (P < .001) and women (P = .014). Both rs671 major G allele and wGRS were not associated with hyperuricemia. In Mendelian randomization analysis, the causal relationship between any alcohol consumption and hyperuricemia was found only in men, albeit non‐significant after correction for multiple testing. The associations did not change after excluding heavy drinkers or the elderly.ConclusionsThese results provide evidence that alcohol consumption is causally associated with risk of hyperuricemia in Korean men and support its role as a risk determinant.
Combining the 5.8S and ITS2 to improve classification of fungi
Authors: Heeger Felix , Wurzbacher Christian , Bourne Elizabeth C. , Mazzoni Camila J. , Monaghan Michael T. 
Published: July 2019

Abstract

The internal transcribed spacer (ITS) is used often in DNA metabarcoding of fungi. One disadvantage of its high variability may be a failure to classify operational taxonomic units (OTUs) when no similar reference sequence exists. We tested whether the 5.8S region, often sequenced with ITS2 but discarded before analysis, could provide OTU classifications when ITS2 fails.

We used in silico evaluation to compare classification success of 5.8S and ITS2 from the UNITE database when reference sequences of the same species, genus, or family were removed. We then developed an automated pipeline for a combined 5.8S–ITS2 analysis and applied it to mixed environmental samples containing many lineages that are underrepresented in databases.

ITS was clearly superior for species‐level classifications with a complete reference database, but 5.8S outperformed ITS at higher level classifications with an incomplete database. Our combined 5.8S‐ITS2 pipeline classified 3× more fungal OTUs compared to ITS2 alone, particularly within Chytridiomycota (27×) and Rozellomycota (6×).

Missing reference sequences led to the failure of ITS to classify many fungal OTUs at all, and to a significant underestimation of environmental fungal diversity. Using 5.8S to complement ITS classification will likely provide better estimates of diversity in lineages for which database coverage is poor.
Basolateral amygdala is required for reconsolidation updating of heroin‐associated memory after prolonged withdrawal
Authors: Yuan Kai , Cao Lu , Xue Yan‐Xue , Luo Yi‐Xiao , Liu Xiao‐Xing , Kong Fan‐Ni , Tabarak Serik , Liao Fan , Meng Shi‐Qiu , Han Ying , Wu Ping , Bao Yan‐Ping , Zhang Wen , Lu Lin , Shi Jie 
Published: July 2019

AbstractPostretrieval extinction procedures are effective nonpharmacological interventions for disrupting drug‐associated memories. Nonetheless, the conditioned stimulus (CS) memory retrieval‐extinction procedure is ineffective in inhibiting drug craving and relapse after prolonged withdrawal, which significantly undermines its therapeutic potential. In the present study, we showed that, unlike the CS memory retrieval‐extinction procedure, noncontingent heroin injections (unconditioned stimulus [UCS]) 1 hour before the extinction sessions decreased the heroin‐priming‐induced reinstatement, renewal, and spontaneous recovery of heroin seeking after 28 days of withdrawal (ie, remote heroin‐associated memories) in rats. The UCS retrieval manipulation induced reactivation of the basolateral amygdala (BLA) after prolonged withdrawal, and this reactivation was absent with the CS retrieval manipulation. Chemogenetic inactivation of the BLA abolished the inhibitory effect of the UCS memory retrieval‐extinction procedure on heroin‐priming‐induced reinstatement after prolonged withdrawal. Furthermore, the combination of chemogenetic reactivation of BLA and CS retrieval‐extinction procedure resembled the inhibitory effect of UCS retrieval‐extinction procedure on heroin seeking after prolonged withdrawal. We also observed that the inhibitory effect of the UCS retrieval‐extinction procedure is mediated by regulation of AMPA receptor endocytosis in the BLA. Our results demonstrate critical engagement of the BLA in reconsolidation updating of heroin‐associated memory after prolonged withdrawal, extending our knowledge of the boundary conditions of the reconsolidation of drug‐associated memories.
CYP2B6 Genotype‐Dependent Inhibition of CYP1A2 and Induction of CYP2A6 by the Antiretroviral Drug Efavirenz in Healthy Volunteers
Authors: Metzger Ingrid F. , Dave Nimita , Kreutz Yvonne , Lu Jessica B.L. , Galinsky Raymond E. , Desta Zeruesenay 
Published: July 2019

We investigated the effect of efavirenz on the activities of cytochrome P450 (CYP)1A2, CYP2A6, xanthine oxidase (XO), and N‐acetyltransferase 2 (NAT2), using caffeine as a probe. A single 150 mg oral dose of caffeine was administered to healthy volunteers (n = 58) on two separate occasions; with a single 600 mg oral dose of efavirenz and after treatment with 600 mg/day efavirenz for 17 days. Caffeine and its metabolites in plasma and urine were quantified using liquid chromatography/tandem‐mass spectrometry. DNA was genotyped for CYP2B6*4 (785A>G), CYP2B6*9 (516G>T), and CYP2B6*18 (983T>C) alleles using TaqMan assays. Relative to single‐dose efavirenz treatment, multiple doses of efavirenz decreased CYP1A2 (by 38%) and increased CYP2A6 (by 85%) activities (P < 0.05); XO and NAT2 activities were unaffected. CYP2B6*6*6 genotype was associated with lower CYP1A2 activity following both single and multiple doses of efavirenz. No similar association was noted for CYP2A6 activity. This is the first report showing that efavirenz reduces hepatic CYP1A2 and suggesting chronic efavirenz exposure likely enhances the elimination of CYP2A6 substrates. This is also the first to report the extent of efavirenz–CYP1A2 interaction may be efavirenz exposure‐dependent and CYP2B6 genotype‐dependent.
Pregnancy‐associated plasma protein‐A2 levels are increased in early‐pregnancy gestational diabetes: a novel biomarker for early risk estimation
Authors: Dereke J. , Nilsson C. , Strevens H. , Landin‐Olsson M. , Hillman M. 
Published: July 2019

AbstractAimTo determine whether pregnancy‐associated plasma protein‐A2 levels are increased in early pregnancies complicated by gestational diabetes and whether gestation age influences levels. The possible use of pregnancy‐associated plasma protein‐A2 as a pre‐screening biomarker to reduce the need for performing oral glucose tolerance tests in pregnant women was also investigated.MethodsPregnant women were diagnosed with gestational diabetes in early pregnancy after a 2‐hour 75 g oral glucose tolerance test in the catchment area of Skåne University Hospital, Lund, Sweden during 2011–2015 (n = 99). Age‐ and BMI‐matched pregnant women without diabetes were recruited at similar gestational ages from maternal healthcare centres in the same geographical area during 2014–2015 to act as controls (n = 100). Circulating pregnancy‐associated plasma protein‐A2 was analysed in participant serum using commercially available enzyme‐linked immunosorbent assay kits.ResultsCirculating pregnancy‐associated plasma protein‐A2 was increased in women diagnosed with gestational diabetes [13.5 (9.58–18.8) ng/ml] compared with controls [8.11 (5.74–11.3) ng/ml; P < 0.001]. Pregnancy‐associated plasma protein‐A2 was associated with gestational diabetes independent of age, BMI, C‐peptide and adiponectin (P < 0.001). Pregnancy‐associated plasma protein‐A2 as a pre‐screening biomarker to identify women at a decreased risk of gestational diabetes resulted in a negative predictive value of 99.7%, with a sensitivity of 96% and a specificity of 30% at a cut‐off level of 6 ng/ml.ConclusionsThis is the first study to show increased pregnancy‐associated plasma protein‐A2 levels in gestational diabetes. Pregnancy‐associated plasma protein‐A2 also shows promise as a pre‐screening biomarker with the potential to reduce the need for performing oral glucose tolerance tests in early pregnancy. Future prospective cohort studies in a larger group of both high‐ and low‐risk women are, however, needed to further confirm this observation.
Nonalcoholic fatty liver disease increases the risk of gastroesophageal reflux disease: A systematic review and meta‐analysis
Authors: Xue Jinru , Xin Hua , Ren Na , Zhou Changyu , Yang Jinghui , Song Lina , Qin Shaoyou 
Published: July 2019

AbstractBackgroundIncreasing evidence indicates that nonalcoholic fatty liver disease (NAFLD) is linked to an increased risk of extra‐hepatic conditions. However, it is currently uncertain whether NAFLD is associated with the risk of gastroesophageal reflux disease (GERD). We performed a systematic review and meta‐analysis of relevant studies to examine the association between NAFLD and the risk of GERD.MethodsWe searched PubMed, Scopus, Embase and Web of Science from 1 January 1975 to 15 December 2018, using predefined terms to identify cross‐sectional, case‐control and cohort studies investigating the association between NAFLD and GERD.ResultsNine observational studies involving 185 118 subjects were eligible for inclusion in the meta‐analysis. Overall, NAFLD was significantly associated with an increased risk of GERD (random effect OR 1.28; 95% CI: 1.12‐1.44, I2 = 82%). Moreover, the significant association between NAFLD and GERD was consistent both for studies with adjusted OR/HR (n = 6, random effect OR = 1.16, 95% CI: 1.03‐1.30) and those with unadjusted OR/HR (n = 3, random effect OR = 2.09, 95% CI: 1.62‐2.56) as measures of effect. Both funnel plot and Egger's test suggested the existence of publication bias. However, a sensitivity analysis by sequentially omitting each study did not alter the pooled outcome,suggesting the robustness of the association.ConclusionNAFLD is associated with an increased risk of GERD. However, future large and cohort studies are still needed to determine the causal relationship between NAFLD and the risk of GERD.
The role of the nucleus accumbens in learned approach behavior diminishes with training
Authors: Dobrovitsky Veronica , West Mark O. , Horvitz Jon C. 
Published: July 2019

AbstractNucleus accumbens dopamine plays a key role in reward‐directed approach. Past findings suggest that dopamine's role in the expression of learned behavior diminishes with extended training. However, little is known about the central substrates that mediate the shift to dopamine‐independent reward approach. In the present study, rats approached and inserted the head into a reward compartment in response to a cue signaling food delivery. On days 4 and 5 of 28‐trial‐per‐day sessions, D1 receptor antagonist R(+)‐7‐chloro‐8‐hydroxy‐3‐methyl‐1‐phenyl‐2,3,4,5‐tetrahydro‐1H‐3‐benzazepine hydrochloride (SCH23390) infused to the NAc core reduced the probability and speed of cued approach. The disruptive effect of D1 receptor blockade was specific to the nucleus accumbens core and not seen with drug infusions to nearby dopamine target regions. In rats that received drug infusions after extended training (days 10 or 11), accumbens core D1 receptor blockade produced little effect on the expression of the same behavior. These results could have been due to a continued accumbens mediation of cued approach even after the behavior had become independent of accumbens D1 receptors. However, accumbens core ionotropic glutamate receptor blockade disrupted cued approach during early but not late stages of training, similar to the effects of D1 antagonist infusions. The results suggest that with extended training, a nucleus accumbens D1‐dependent behavior becomes less dependent not only on nucleus accumbens D1 transmission but also on excitatory transmission in the nucleus accumbens. These findings fill an important gap in a growing literature on reorganization of striatal function over the course of training.
Volatile anesthetic sevoflurane pretreatment alleviates hypoxia‐induced potentiation of excitatory inputs to striatal medium spiny neurons of mice
Authors: Fukuda Masataka , Ando Nozomi , Sugasawa Yusuke , Inoue Ritsuko , Nakauchi Sakura , Miura Masami , Nishimura Kinya 
Published: July 2019

AbstractSevoflurane, a commonly used anesthetic in surgery, has drawn attention because of its preconditioning effects in hypoxic conditions. To investigate the preconditioning effects in the striatum, a common site for ischemic stroke, we collected whole‐cell current‐clamp recordings from striatal medium spiny neurons. In our in vitro brain slice experiments, deprivation of oxygen and glucose depolarized the striatal neurons to subthreshold potentials, and the pre‐administration of sevoflurane (4%, 15 min) prolonged the time to depolarization. Furthermore, transient hypoxia induced the potentiation of excitatory postsynaptic potentials, which play a part in post‐ischemic excitotoxicity. Glibenclamide, a KATP channel inhibitor, reversed the prolonged time to depolarization and the prevention of the pathological potentiation of excitatory responses, indicating that the short exposure to sevoflurane likely participates in neuroprotection against hypoxia via activation of KATP channels. A monocarboxylate transporter blocker, 4‐CIN, also depolarized striatal neurons. Interestingly, the blockade of monocarboxylate transporters that supply lactate to neurons caused the pathological potentiation, even in the presence of enough oxygen and glucose. In this case, sevoflurane could not prevent the pathological potentiation, suggesting the involvement of monocarboxylate transporters in the sevoflurane‐mediated effects. These results indicate that sevoflurane protects striatal neurons from hypoxic damage and alleviates the pathological potentiation. Under these conditions, sevoflurane may become an effective intervention for patients undergoing surgery.
Cholinergic modulation of striatal nitric oxide‐producing interneurons
Authors: Melendez‐Zaidi Alexandria E. , Lakshminarasimhah Harini , Surmeier Dalton James 
Published: July 2019

AbstractStriatal GABAergic interneurons that express nitric oxide synthase—so‐called low‐threshold spike interneurons (LTSIs)—play several key roles in the striatum. But what drives the activity of these interneurons is less well defined. To fill this gap, a combination of monosynaptic rabies virus mapping (msRVm), electrophysiological and optogenetic approaches were used in transgenic mice in which LTSIs expressed either Cre recombinase or a fluorescent reporter. The rabies virus studies revealed a striking similarity in the afferent connectomes of LTSIs and neighboring cholinergic interneurons, particularly regarding connections arising from the parafascicular nucleus of the thalamus and cingulate cortex. While optogenetic stimulation of cingulate inputs excited both cholinergic interneurons and LTSIs, thalamic stimulation excited cholinergic interneurons, but inhibited LTSIs. This inhibition was dependent on cholinergic interneurons and had two components: a previously described GABAergic element and one that was mediated by M4 muscarinic acetylcholine receptors. In addition to this phasic signal, cholinergic interneurons tonically excited LTSIs through a distinct, M1 muscarinic acetylcholine receptor pathway. This coordinated cholinergic modulation of LTSIs predisposed them to rhythmically burst in response to phasic thalamic activity, potentially reconfiguring striatal circuitry in response to salient environmental stimuli.
Under‐ice metabolism in a shallow lake in a cold and arid climate
Authors: Song Shuang , Li Changyou , Shi Xiaohong , Zhao Shengnan , Tian Weidong , Li Zhijun , Bai Yila , Cao Xiaowei , Wang Qingkai , Huotari Jussi , Tulonen Tiina , Uusheimo Sari , Leppäranta Matti , Loehr John , Arvola Lauri 
Published: July 2019

Abstract

Winter is a long period of the annual cycle of many lakes in the northern hemisphere. Low irradiance, ice, and snow cover cause poor light penetration into the water column of these lakes. Therefore, in northern lakes, respiration often exceeds primary production leading to low dissolved oxygen concentrations. This study aimed to quantify under‐ice metabolic processes during winter in an arid zone lake with little snow cover.

This study was carried out in a mid‐latitude lake in Inner Mongolia, northern China. The study lake receives relatively high incoming solar radiation on the ice in mid‐winter, and radiation can penetrate down to the bottom sediment as the lake is shallow and the ice lacks snow cover.

Primary production and respiration were estimated during two winters using high‐frequency sensor measurements of dissolved oxygen. To quantify under‐ice metabolic processes, sensors were deployed to different depths. During both winters, sensors collected data every 10 min over several weeks.

The amount of solar radiation controlled photosynthesis under ice; temperature and photosynthesis together appeared to control respiration. The balance between gross primary production and ecosystem respiration was especially sensitive to changes in snow cover, and the balance between P and R decreased.

Our data suggest that photosynthesis by plankton, submerged plants, and epiphytic algae may continue over winter in shallow lakes in mid‐latitudes when there is no snow cover on the ice, as may occur in arid climates. The continuation of photosynthesis under ice buffers against dissolved oxygen depletion and prevents consequent harmful ecosystem effects.