bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of the RNA Regulator HuR mitigates spinal cord injury by potently suppressing post-injury neuroinflammation
Authors: Husain Mohammed Amir , Smith Reed , Sorge Robert E. , Kaimari Abdulraheem , Si Ying , Hassan Ali Z. , Guha Abhishek , Smith Katherine A. , Cardozo Christopher P. , DeBerry Jennifer J. , Andrabi Shaida A. , Nabors L. Burt , Filippova Natalia , Webb Caroline K. , King Peter H. 
Published: October 2024

AbstractBackgroundNeuroinflammation plays a significant role in promoting secondary tissue injury after spinal cord trauma. Within minutes after spinal cord injury (SCI), microglia and astrocytes become activated and produce inflammatory mediators such as TNF-α, IL-6, iNOS and COX-2 which induce tissue injury through cytotoxicity, vascular hyperpermeability, and secondary ischemia. The inflammatory cascade is amplified by chemokines such as CCL2 and CXCL1 that promote recruitment of peripheral inflammatory cells into the injured spinal cord. HuR is a key post-transcriptional RNA regulator that controls glial expression of many pro-inflammatory factors by binding to adenylate- and uridylate-rich elements in 3’ untranslated regions of the mRNA. SRI-42127 is a small molecule inhibitor that blocks HuR nucleocytoplasmic translocation, a process critical for its regulatory function. The goal of this study was to assess the potential of SRI-42127 for suppressing neuroinflammation after SCI and improving functional outcome.MethodsAdult female mice underwent a contusion injury at the T10 level. SRI-42127 or vehicle was administered intraperitoneally starting 1 h after injury and up to 5 days. Locomotor function was assessed by open field testing, balance beam and rotarod. Immunohistochemistry was used to assess lesion size, neuronal loss, myelin sparing, microglial activation and HuR localization. Molecular analyses of spinal cord and peripheral tissues for expression of inflammatory mediators included qPCR, immunohistochemistry, ELISA, or western blot. Post-SCI pain was assessed by the mouse grimace scale.ResultsSRI-42127 significantly attenuated loss of locomotor function and post-SCI pain. Histologic correlates to these beneficial effects included reduced lesion size, neuronal loss, and an increase in myelin sparing. There was reduced microglial activation at the epicenter with concomitant attenuation of HuR nucleocytoplasmic translocation. Molecular analysis revealed a striking reduction of pro-inflammatory mediators at the epicenter including IL-6, MMP-12, IL-1β, TNF-α, iNOS, COX-2, and chemokines CCL2, CXCL1, and CXCL2. Suppression of inflammatory responses extended peripherally including serum, liver, and spleen.ConclusionTargeting HuR after SCI is a viable therapeutic approach for suppressing neuroinflammatory responses after tissue injury and improving functional outcome.
Alternatives to Friction Coefficient: Fine Touch Perception Relies on Frictional Instabilities
Authors: Derkaloustian Maryanne , Bhattacharyya Pushpita , Ngo Truc , Cashaback Joshua G. A. , Medina Jared , Dhong Charles B. 
Published: October 2024

AbstractFine touch perception is often correlated to material properties and friction coefficients, but the inherent variability of human motion has led to low correlations and contradictory findings. Instead, we hypothesized that humans use frictional instabilities to discriminate between objects. We constructed a set of coated surfaces with physical differences which were imperceptible by touch but created different types of instabilities based on how quickly a finger is slid and how hard a human finger is pressed during sliding. We found that participant accuracy in tactile discrimination most strongly correlated with formations of steady sliding, and response times negatively correlated with stiction spikes. Conversely, traditional metrics like surface roughness or average friction coefficient did not predict tactile discriminability. Identifying the central role of frictional instabilities as an alternative to using friction coefficients should accelerate the design of tactile interfaces for psychophysics and haptics.
String-pulling by the common marmoset
Authors: Bertrand Mathilde , Karkuszewski Michael , Kersten Rhonda , Orban de Xivry Jean-Jacques , Pruszynski J. Andrew 
Published: October 2024

AbstractCoordinated hand movements used to grasp and manipulate objects are crucial for many daily activities, such as tying shoelaces or opening jars. Recently, the string-pulling task – which involves cyclically reaching, grasping and pulling a string – has been used to study coordinated hand movements in rodents and humans. Here we characterize how adult common marmosets perform the string-pulling task and describe changes in performance across the lifespan. Marmosets (n=15, 7 female) performed a string-pulling task for a food reward using an instrumented apparatus attached to their home-cage. Movement kinematics were acquired using markerless video tracking and we assessed individual hand movements and bimanual coordination using standard metrics. Marmosets oriented their gaze towards the string above their hands and readily performed the task regardless of sex or age. The task required very little training and animals routinely engaged in multiple pulling trials per session despite not being under water or food control. All marmosets showed consistent pulling speed and similar hand movements regardless of age. Adult marmosets exhibited a clear hand effect, performing straighter and faster movements with their right hand despite showing idiosyncratic hand preference according to a traditional food retrieval assay. Hand effects were also evident for younger animals but seemed attenuated in the older animals. In terms of bimanual coordination, all adult marmosets demonstrated an alternating movement pattern for vertical hand positions. Two younger and two older marmosets exhibited idiosyncratic coordination patterns even after substantial experience. In general, younger and older animals exhibited higher variability in bimanual coordination than did adults.
Loss of Calcitonin Gene Related Receptor component protein (RCP) in nervous system can bias “gepant” antagonism
Authors: Rahman Shafaqat M. , Dickerson Ian , Luebke Anne E. 
Published: October 2024

AbstractWe examined calcitonin gene-related peptide (CGRP)’s effects on behavioral surrogates for motion-induced nausea and static imbalance in the nestinRCP (-/-), a novel mouse model that loses expression of receptor component protein (RCP) in the nervous system after tamoxifen induction. The assays used were the motion-induced thermoregulation and center of pressure (CoP) assays. Findings suggest CGRP’s affects behavioral measures in the nestinRCP (-/-) similarly to littermate controls, since CGRP was observed to increase female sway and diminishes tail vasodilations to provocative motion in both sexes. However, the CGRP-receptor antagonist olcegepant did not antagonize CGRP’s effects in the nestinRCP (-/-), whereas it was effective in littermate controls. Findings suggest RCP loss may change the sensitivity of the CGRP receptor and affect the efficacy of receptor antagonists.Significance StatementResearch in calcitonin gene-related peptide (CGRP) has primarily focused on ligand- receptor interactions at the calcitonin-like receptor (CLR) and receptor activity-modifying unit 1 (RAMP1) subunits. However, the role of receptor component protein (RCP), which mediates signaling via the Gα-stimulatory pathway, is less understood. A novel tamoxifen-inducible mouse model, nestinRCP (-/-), was generated to study loss of RCP in CGRP signaling in the nervous system, and behavioral changes to motion-induced nausea and postural sway were studied after systemic injections of CGRP or CGRP co-delivered with migraine drugs. Findings from this study suggest the loss of CGRP-RCP can bias “gepant” antagonists like olcegepant, and may promote development of therapies to inhibit the RCP-CLR interactions.
Posterior parietal cortex mediates rarity-induced decision bias and learning under uncertainty
Authors: Sheng Weihao , Huang Xinrui , Xie Yang , Tsakiris Manolis C. , Yang Yang 
Published: October 2024

ABSTRACTMaking decisions when outcomes are uncertain requires judgment of outcome probability, yet such judgments are often inaccurate, owing to reliance on heuristics that introduce systematic errors like overweighting of low probabilities. Here, using a decision-making task in which the participants were unaware of outcome probabilities, we discovered that both humans and mice exhibit a rarity-induced decision bias (RIDB),i.e., a preference towards rare rewards, which persists throughout task performance. Optogenetics experiments demonstrated that activity in the posterior parietal cortex (PPC) is required for the RIDB. Usingin vivoelectrophysiology, we found that rare rewards bidirectionally modulate choice-encoding PPC neurons to bias subsequent decisions towards rare rewards. Learning dynamically recruits PPC neurons and enhances their stimulus encoding, and the activity of these neurons is essential for stimulus-guided decisions. We then developed a dual-agent model that successfully recapitulates the decision-making and learning behaviours, and corroborates the specific functions of PPC neurons in mediating decision-making and learning. This study not only extends our understanding of rare probability overweighting to a context where the outcome probability is unknown and characterizes the neural basis for RIDB in the PPC, but moreover reveals an evolutionarily conserved heuristic that persistently impacts decision-making and learning under uncertainty.
Characterization of Hippo Signaling Components in the Early Dorsal Pancreatic Bud
Authors: Ahuja Neha , Maynard Caitlin , Bierschenck Tyler , Cleaver Ondine 
Published: October 2024

ABSTRACTAll pancreatic lineages originate from a transitory structure known as the multipotent progenitor epithelium (MPE), which is a placode formed via epithelial stratification. Cells within the MPE undergode novolumenogenesis to give rise to an epithelial plexus, which serves as a progenitor niche for subsequent development of endocrine, ductal and acinar cell types. Recent evidence suggests that Hippo signaling is required for pancreatic cell differentiation, but little is known about the function of Hippo signaling in the development of the MPE. Here, we characterize the expression of YAP1, TAZ, and the Hippo regulators LATS1/2 kinases and MERLIN in early murine pancreatic epithelium, during epithelial stratification, plexus development and emergence of endocrine cells. We find that YAP1 expression is relatively low in the pancreas bud during stratification, but increases by E11.5. Intriguingly, we find that TAZ, but not YAP1, is expressed in early endocrine cells. We further find that MERLIN and LATS1/2 kinases are robustly expressed during the period of rapid stratification and become markedly apical at nascent lumens. To gain a better understanding of how Hippo signaling and lumen formation are connected, we analyzed the expression of Hippo signaling components in anin vitromodel of lumen formation and found that they are dynamically regulated during lumenogenesis. Together, our results point to a relationship between Hippo signaling and lumen formation during pancreatic development.HIGHLIGHTSYAP1 expression in the early mouse pancreatic anlagen is low until approximately E11.5, when it becomes localized to cell nuclei in multipotent progenitor cells. At E14.5, we find nuclear YAP1 in ductal cells.YAP1 is not expressed in early and midgestation endocrine cells. By contrast, TAZ is expressed in first transition endocrine cells.Hippo regulators MERLIN and LATS1/2 kinases are robustly expressed in the early pancreatic bud by E10.5. Both MERLIN and LATS1/2 exhibit strong apical localization in epithelial cells at nascent microlumens.Usingin vitromodels ofde novopancreas lumen formation, we show that YAP1 nuclear localization is high in early phases of lumen formation and gradually decreases as lumens matures.
‘Mini analysis’ is an unreliable reporter of synaptic changes
Authors: Greger Ingo H. , Watson Jake F. 
Published: October 2024

AbstractAnalysis of miniature postsynaptic currents (mPSCs, or ‘minis’) is one of the most extensively employed approaches to determine the functional properties of synaptic connections. The popularity of this technique stems from its simplicity. Patch-clamp recording is used to observe spontaneous transmission occurring at synapses throughout a neuron’s dendritic tree. These events are analysed in an attempt to determine quantal synaptic parameters and changes during experimental manipulation. For decades, changes in the amplitude or frequency of mPSCs have been interpreted as evidence for specific synaptic modifications, including changes in presynaptic release sites, postsynaptic receptor abundance or even nanoscale changes in the alignment of synaptic machinery. However, at the majority of brain synapses, these events are small, with many undetectable due to recording noise. Here, using both simulated and experimental data, we demonstrate that interpreting synaptic changes from mPSC datasets is fundamentally fallible. Due to incomplete detection of event distributions, seemingly specific changes in mPSC amplitude or frequency falsely report actual synaptic changes. In addition, probabilistic detection of small events gives false confidence in the completeness of detection and inaccurate determination of quantal size. We not only demonstrate the dense pitfalls of mini analysis, but also establish a method for experimental detection of the detection limit, allowing more robust data analysis and scientific interpretation.
Decoding Olfactory Bulb Output: A Behavioral Assessment of Rate, Synchrony, and Respiratory Phase Coding
Authors: Fu Xiaochen , Reinert Janine K. , Lindeman Sander , Fukunaga Izumi 
Published: October 2024

AbstractThe olfactory system is a well-known model for studying the temporal encoding of sensory stimuli due to its rhythmic stimulus delivery through respiration. The sniff rhythm is considered critical to structuring the output of computation in the primary olfactory area, the olfactory bulb. Here, Neuropixels recording from awake, head-fixed mice confirmed that both the rate and sniff-locked spike timing are informative about odour identity. We tested the behavioural importance of these temporal features using simple closed-loop optogenetics embedded in custom behavioural paradigms. We found that mice perceive differences in evoked spike counts and discriminate between synchronous vs. asynchronous activations of the output neurons. Surprisingly, they failed to distinguish the timing of evoked activity relative to the sniff cycle. These results challenge the utility of internally generated rhythms as reference signals in the neural encoding of the environment.
How to establish customers’ green brand relationships and behaviors: from green consumption consciousness perspectives
Authors: Hsu Li-Chun 
Published: October 2024

PurposeThis study aimed to understand how the consumer–green brand relationship affects the behavior of consumers of e-commerce platforms for smallholder farmers; it did so by formulating and testing a framework based on social identity perspective.Design/methodology/approachStructural equation modeling was applied to data from 532 questionnaire responses.FindingsAll hypotheses were supported. Customers’ environmental consciousness, green brand consciousness and green brand self-expression strongly affect green consumption consciousness. The effect of environmental consciousness on green brand identification is stronger than that of environmental consciousness on green brand consciousness and that of green brand self-expression on green brand identification.Practical implicationsSuggestions for managing green brands on e-commerce platforms for smallholder farmers and references for strategizing are also provided.Originality/valueThis study accounts for a wide range of antecedents of green consumption consciousness based on a social identity perspective. This study is the first to elucidate how green consumption affects the e-commerce branding of green smallholder farmers.
Decarbonization and waste reduction in a disrupted food supply chain: a step towards achieving food sustainability
Authors: Rana Ranveer Singh , Kumar Dinesh , Prasad Kanika 
Published: October 2024

PurposeThis study aims to reduce carbon emissions and minimize waste in the event of disruptions in a short and fast-food perishable such as fruits, vegetables, packaged food items, etc. supply chain through optimal investment in green and preservation technologies.Design/methodology/approach This study utilized a Hessian matrix approach to optimize decision variables with an objective to maximize the profit function.FindingsThe study demonstrates that investing in both green and preservation technology within a short and fast-food supply chain is highly beneficial for decarbonization and waste reduction and it leads to profit maximization. It has been shown with the help of a numerical experiments with investment in both green and preservation technology that total profit is 3.09% higher than without investment made in either technology.Practical implicationsThis study aids the industry in achieving food sustainability by minimizing waste of perishables and also minimizes carbon emissions which is essential for environmental protection. It assists industries in determining the optimal investment in preservation technology to minimize waste and in green technology to reduce emissions, thereby maximizing profits.Originality/valueThe current study formulates an inventory model that helps in decarbonization and waste reduction in food supply chain with the consideration of machine learning, demand disruption, preservation technology investment, screening of purchased items, waste disposal, a double triangular distribution deterioration rate, green technology investment, carbon emissions from various supply chain activities, carbon tax policy and fuel price variation over time for perishable food products in a two-warehouse system.
Deciphering consumer behaviours in the innovative hospitality settings
Authors: Magdy Ahmed , Hassan Hamada Gamal 
Published: October 2024

Purpose
This study aims to analyse the antecedents and consequences of consumer participation behaviour in the hotel industry. It investigates how citizenship behaviour influences the link between participation and switching intention. It also investigates hotel innovativeness, brand identification and consumer value co-creation using the frameworks of innovation diffusion theory, social identity theory and social exchange theory. By combining different academic perspectives, the study provides a thorough grasp of the elements that influence consumer behaviour and loyalty to hospitality brands.

Design/methodology/approach
This study used a descriptive-analytical approach to survey 526 guests who stayed at five-star hotels affiliated with chains in Egypt. The hotels were chosen using stratified random sampling, which ensured representation across multiple segments. For practical reasons, convenience sampling techniques were used to pick research participants. To test the study hypotheses, structural equation modelling was used, which provided a strong statistical analysis to assess the correlations between variables and validate the suggested theoretical framework.

Findings
The research indicates that hotel innovativeness has a substantial impact on consumers’ brand identification and involvement behaviour, but not their propensity to move brands. The relationship between participation behaviours and intention to switch is mediated by consumer citizenship behaviour.

Research limitations/implications
The cross-sectional study methodology may not address causal linkages or developmental difficulties because it only collected data at one point in time. A longer study period is required, and the sample group’s breadth is restricted to five-star hotel guests from Egypt’s chain-affiliated hotels.

Originality/value
This study offers a thorough methodology for assessing how hotel innovation influences consumer brand identification. It also looks at how consumer brand identification affects involvement and switching intentions. The study also investigates the moderating influence of consumer citizenship behaviour in the link between involvement and switching intentions.
A hybrid conceptual procurement framework for BIM uptake to enhance buildings' sustainability performance in the Jordanian public sector
Authors: Alhusban Mohammad , Nasereddin Mohammad , Alghossoon Abdullah , Hatamleh Muhammad T. 
Published: October 2024

PurposeA large portion of the public building projects in Jordan suffers from sustainability performance issues. Therefore, this research aims to develop a hybrid procurement framework to enhance the implementation of BIM in the Jordanian public sector for better sustainable building performance. This study also aims to identify the influence of procurement factors on BIM adoption and usage in the public sector in Jordan and to provide a holistic picture of recent research on technology acceptance and adoption in the construction industry.Design/methodology/approachA qualitative approach was adopted using semi-structured interviews with key stakeholders (n = 12) in Jordan to explore procurement routes issues facing the adoption of BIM and to find proposed measures. Then, the findings of the interviews analysis using conventional content analysis and literature review were used based on a problem-solving approach to develop a hybrid conceptual procurement framework for BIM uptake to enhance the sustainability of building performance. Subsequently, semi-structured interviews were conducted to validate and refine the proposed conceptual procurement framework.FindingsIt was revealed that the deployment of unfavourable construction procurement approaches represents a major hurdle towards BIM implementation. Though essential for enhancing BIM implementation, it is revealed that a fundamental change from the common Design bid build (DBB) to more collaborative procurement approaches remains infeasible in view of the realities that govern the construction industry. Moreover, this study has found the key procurement approach challenges for effective BIM implementation.Research limitations/implicationsResearch is limited to the public sector in Jordan and further qualitative and quantitative testing of the conceptual procurement framework is needed to adjust the framework before large-scale testing.Originality/valueThe developed conceptual procurement framework is the first of its kind and draws a set of procurement approach remedies for the Jordanian public sector to foster the implementation of BIM.