bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antimicrobial resistance and novel mutations detected in the gyrA and parC genes from Pseudomonas aeruginosa strains isolated from companion dogs
Authors: Park Youjin , Oh Jaeyoung , Park Sowon , Sum Samuth , Song Wonkeun , Chae Jongchan , Park Hee-Myung 
Published: August 2019

Abstract
Background

Fluoroquinolone agents, such as enrofloxacin and marbofloxacin, are commonly used in pseudomonal infection in veterinary medicine. However, the resistance rate to fluoroquinolone is rapidly increasing according to multiple studies in various countries. The point mutation in quinolone resistance determining region (QRDR) is closely related to increased fluoroquinolone resistance of Pseudomonas aeruginosa . The aim of this study was to investigate current antimicrobial susceptibility and fluoroquinolone resistance in Pseudomonas aeruginosa strains isolated from dogs. The presence of the point mutations in the QRDR was confirmed by gyrA and parC polymerase chain reaction and nucleotide sequencing analysis.

Results

A total of 84 nonduplicated P. aeruginosa isolates were obtained from 228 healthy dogs (healthy group) and 260 dogs with clinical signs (infected group). From these, 38 isolates from the healthy group were detected in several samples, whereas 46 isolates from the infected group were mostly obtained from dogs’ ears with otitis externa (41/260, 15.8%). All isolates were resistant to nalidixic acid, while some were also resistant to enrofloxacin (23/84, 27.4%), marbofloxacin (17/84, 20.2%), levofloxacin (12/84, 14.3%), or ciprofloxacin (11/84, 13.1%). Enrofloxacin resistance was significantly higher in strains from the infected group than that of the healthy group ( p <0.05). Among the 23 fluoroquinolone-resistant isolates, 8 and 4 different mutations were detected in the gyrA and parC genes, respectively. Mutations in gyrA were significantly common in the infected group ( p <0.05). Hotspots for the gyrA and parC mutations were Thr83 (34.8%, 8/23) and Pro116 (91.3%, 21/23), respectively. Double and triple mutations were also found in 5 of the isolates.

Conclusion

Novel mutations in the gyrA and parC genes were first found in P. aeruginosa isolates from companion dogs in South Korea. These findings suggest that it is important to know the prudent use of fluoroquinolone antibiotics in canine pseudomonas infection treatment.
Factors associated with progression to severe condition and treatment prolongation in respiratory syncytial virus infection: a retrospective study in Japan
Authors: Oikawa Kosuke , Suzuki Manabu , Terada Tomomasa , Koganesawa Masaya , Sakurai Motoichiro , Matsuhashi Kazuhiko , Ochiai Hirotaka , Kokaze Akatsuki , Mizuno Katsumi 
Published: August 2019

Abstract
Overview
Background: In recent years, the epidemic of respiratory syncytial virus (RSV) has been seen in the summer. In daily clinical practice, we noted that RSV infection tends to become more severe and its treatment tends to be longer, particularly in patients hospitalized in the summer. Thus, we investigated factors associated with the progression to severe condition and the prolongation of treatment in RSV infection.

Methods: Subjects were pediatric patients diagnosed with RSV infection and hospitalized for treatment during the 5 year s from April 2014 to March 2019. Information on age, sex, season of hospitalization, gestational age, and bronchial asthma of each subject was collected from inpatient medical records. Duration of oxygen administration was used as an index for treatment duration and the use of devices for severe cases as an index for severity. Multiple linear regression analysis and logistic regression analysis were performed to examine the variables associated with duration of oxygen administration and use of a device for severe cases.

Results: Data from 298 patients were analyzed. Duration of oxygen administration was significantly associated with bronchial asthma (partial regression coefficient: 0.804, p = 0.010). Hospitalization in summer was significantly associated with use of a device for severe condition (adjusted odds ratio: 5.89, 95% confidence interval: 1.72-20.18).

Conclusion: The present study showed that bronchial asthma is a risk factor for prolongation of treatment and infection in summer is a risk factor for progression to severe condition in cases of RSV infection. These findings suggested that children with bronchial asthma and infection in summer need to be treated carefully regardless of their age, sex, and gestational age.
Self-assembling Rotavirus VP6 Nanoparticle Vaccines Expressed in Escherichia coli Elicit Systemic and Mucosal Responses in Mice
Authors: Li Zhipeng , Cui Kuiqing , Huang Kongwei , Liu Fuhang , Shi Deshun , Liu Qingyou 
Published: August 2019

Background:
Rotavirus is the most common cause of infectious diarrhea in infants and
young children around the world. The inner capsid protein VP6 has been discussed as alternative
vaccine as it can induce cross-protective immune responses against different RV strai. The use of
ferritin nanoparticle may enhance the immunogenicity of the subunit vaccine.

Objective:
In this article, our motivation is to design and obtain a self-assemble rotavirus
nanoparticle vaccine which can induce efficiency immune response.

Methods:
The VP6 protein was fused with ferritin and expressed in the Escherichia coli expression
system. The recombinant VP6-ferritin (rVP6-ferritin) protein was purified by His-tag affinity
chromatography and fast protein liquid chromatography. Transmission electron micrographic
analysis was used to detect the nanostructure of the self-assembled protein. Mice were gavage with
the protein and ELISA was used to detect the titer of the VP6 specific antibody.

Results:
The recombined VP6-ferritin was expressed in the Escherichia coli as an inclusion body
form and the purified protein has similar antigenicity to rotavirus VP6. Transmission electron
micrographic analysis of rVP6-ferritin exhibited spherical architecture with a uniform size
distribution, which is similar to the ferritin nanocage. Immune response analysis showed that mice
immunized by rVP6-ferritin protein induced 8000 (8000±1093) anti-VP6 IgG titers or 1152
(1152±248.8) anti-VP6 IgA titers.

Conclusion:
According to the above research, the rotavirus VP6-ferritin protein can be easily
express and self-assemble to the nano-vaccine and induce efficiency humoral and mucosal
immunity. Our research makes a foundation for the development of oral rotavirus vaccine.
Functional Changes of Paneth Cells in the Intestinal Epithelium of Mice with Obstructive Jaundice and After Internal and External Biliary Drainage
Authors: Tian Xiaopeng , Zhang Zixuan , Li Wen 
Published: August 2019

Objective:To investigate the functional changes of Paneth cells in the intestinal epithelium of mice with obstructive jaundice (OJ) and after internal biliary drainage (ID) and external biliary drainage (ED).Methods:The experiment was divided into two stages. First stage: Mice were randomly assigned to two groups: (I) sham operation (SH); (II) OJ. The mice were sacrificed before the operation and on the 1st, 3rd, 5th and 7th day after the operation to collect specimens. Second stage: Mice were randomly assigned to four groups: (I) SH; (II) OJ; (III) OJ and ED; and (IV) OJ and ID. They were reoperated on day 5 for biliary drainage procedure. The specimens were collected on day 10.Results:The expressions of lysozyme and cryptdin-4 increased first and then decreased over time in group OJ, and the number of Paneth cells decreased gradually with the extension of OJ time（p<0.05. After the secondary operation on the mice to relieve OJ, the number of Paneth cells and expressions of lysozyme and cryptdin-4 in group ID increased more significantly than those in group ED（p<0.05）.Conclusion:OJ could cause intestinal Paneth cells to dysfunction in mice. ID was more significant than ED in restoring the function of Paneth cells. It might be one of the mechanisms that make ID superior to ED.
Pyrrolo[1,2-a]azepines Coupled with Benzothiazole and Fluorinated Aryl Thiourea Scaffolds as Promising Antioxidant and Anticancer Agents
Authors: Patel Rahul V. , Mistry Bhupendra M. , Syed Riyaz , Parekh Nikhil M. , Shin Han-Seung 
Published: August 2019

Background:
Cancer remains a major health concern throughout history and is responsible for huge
numbers of deaths globally. The sensitivity of cancer cells to anticancer drugs is a crucial factor for developing
effective treatments.

Methods:
Pyrrolo[1,2-a]azepines coupled with benzothiazole and fluorinated aryl thiourea scaffolds have been
synthesized, and their potential as cytotoxic agents was investigated against different cancer cell lines such as
human ovarian cancer (SK-OV-3), cervical cancer (HeLa), colon adenocarcinoma (HT-29) and non-small-cell
lung carcinoma (A549). Cytotoxicity of new compounds was confirmed using SRB assay against non-cancer
MDCK cell line. In addition, free radical scavenging activity of new pyrrolo[1,2-a]azepines was examined by
adopting DPPH and ABTS assays.

Results:
The results concluded that the presence and position of fluorine atom(s) on the thiourea unit played a
significant role in order to gain anticipated efficacies. Results of the cytotoxic assay against non-cancer MDCK
cells showed that these new derivatives are safe to study further. New structures were confirmed using spectral
and elemental analyses.

Conclusion:
Pyrrolo[1,2-a]azepines endowed with a benzothiazole entity and fluorinated aryl thiourea substituents
were derived aiming to furnish remarkable antioxidant and anticancer activities. New molecules generated
showed interesting biological result correlated with the structure and substituent of the final derivatives. Specifically,
numbers and position of fluorine atoms on the thiourea unit influenced the biological profile of the mentioned
compounds.
Properties of Modified High Permeable Concrete with a Crumb Rubber
Authors: Ng Cheng Yee , Narong Anaqi Ratna , Zaman Aifaa Balqis Kamarul , Mustaffa Zahiraniza , Mohammed Bashar S. , Ean Lee Woen 
Published: August 2019

Background:
High permeable concrete is designed with high porosity, which allows water to pass through it. By considering this ability, it has been widely used for various applications including high permeable road pavement. However, to fully adopt highly permeable road pavement technology is challenging due to high cost and maintenance, besides the present engineers and contractors have insufficient expertise and experience with this technology. Henceforth, the high porosity property is highly favorable for road curb applications especially in the tropical region like Malaysia with high rainfall intensity.

Objective:
This paper aimed to determine the properties of the concrete experimentally, and propose the optimum mix design of high permeable concrete for road curb.

Methods:
The flow value and setting time of the cement paste were investigated. Next, the effects of the modified high permeable concrete with varying amount of crumb rubber on compressive strength, permeability and void ratio were also investigated.

Results and Conclusion:
Results have revealed that the workability of the cement paste increases when the water-binder ratio and the amount of fly ash increase. Meanwhile, an increasing amount of cement ratio and crumb rubber lowers the flow value and consequently leads to lower void ratio and permeability. Besides, it has been found that the higher the compressive strength, the lower the void ratio and permeability of the concrete. The research findings support that using an optimum amount of cement replacement with crumb rubber as an additive may increase the performance a high permeable concrete. Herein, the results indicate that the mix design of the concrete studied has the potential to be applied onto the road curb.
In vitro Assessment of Peri-implantitis Treatment Procedures: A Review
Authors: Benli Merve , Petit Catherine , Tenenbaum Henri , Huck Olivier 
Published: August 2019

Background:
The prevalence of peri-implantitis is increasing continuously and such a biological complication significantly decreases implant survival and success. Although various treatment modalities have been identified for peri-implantitis, no completely efficient method has yet been established.

Objective:
The aim of this review was to evaluate the scientific literature regarding the in vitro effects of peri-implantitis treatment.

Methods:
A review of the literature was performed by using Google Scholar, PubMed/ MEDLINE and Science Direct databases. In vitro studies on peri-implantitis treatment modalities were selected. The search strategy identified 57 eligible studies. After selection, 21 articles met all the inclusion criteria and were included in the present review.

Results:
Included in vitro studies evaluated different types of peri-implantitis treatment modalities such as mechanical, chemical, combination and laser therapies. Combination therapies with the aid of adjuvants were found to be more effective compared to the studies that used only one type of treatment modality. Laser systems were also tested and displayed interesting results in terms of surface decontamination with a variability associated with selected parameters.

Conclusion:
This review was performed to evaluate the efficacy of the treatment modalities used for peri-implantitis in vitro. Although there are various effective treatment methods, none has been completely successful in removing the biofilms related to peri-implantitis. The findings imply the need for further studies to develop more effective antimicrobial treatment procedures.
Awareness of the Relationship between Systemic and Periodontal Diseases among Physicians and Dentists in Saudi Arabia and Kuwait: Cross-sectional Study
Authors: Al Sharrad Ahmad , Said Khalid N. , Farook Fathima F. , Shafik Sami , Al-Shammari Khallaf 
Published: August 2019

Background:Previous studies have shown possible association between periodontal diseases and Diabetes Mellitus (DM), Cardiovascular Disease (CVD) and Adverse Pregnancy Outcomes (APO). Increasing the knowledge of physicians and dentists regarding the association between periodontal disease and systemic diseases should improve their ability to provide a high standard of treatment for the disease and prevent its consequences. In our study, we sought to identify knowledge gaps in the awareness of the relationship between systemic and periodontal diseases among dentists and physicians from the Kingdom of Saudi Arabia and the State of Kuwait.Methods:A cross-sectional study was performed with a convenient sample of selected physicians and dentists in Saudi Arabia and Kuwait using a self-developed structured questionnaire. Physicians’ and dentists’ awareness were assessed regarding current evidence of the relationship between systemic diseases and conditions (DM, CVD, APO) and periodontal diseases.Results:A total of 134 (21.5%) of the identified physicians and 485 (78%) of the identified dentists participated in this study. More than half of the participants (52.1% physicians and 67.5% dentists) agreed that there is a relationship between periodontal diseases and DM, CVD and APO. Dentists had a statistically higher level of awareness compared with the physicians. The findings related to the awareness of the dentist sub groups highlighted that periodontists were significantly more likely to have a good level of knowledge.Conclusion:Within the limitations of this study, we concluded that the awareness of physicians and dentists regarding the relationship between periodontal diseases and systemic diseases (DM, CVD, APO) is generally low, Therefore, improved collaboration between medical and dental health providers is recommended.
Does the Trace Element Deficiency (Vit A, D & Zinc) Have Any Role in Vulnerability to Urinary Tract Infection in Children: A Case-Control Study: Tehran, Iran
Authors: Noorbakhsh Samileh , Nia Shima J. , Movahedi Zahra , Ashouri Sarvenaz 
Published: August 2019

Background and Objective:
Urinary Tract Infection (UTI) is one of the most common causes of hospital admission in our young population. This prospective cohort study was carried out to assess the relation of serum levels of zinc, vitamins A and D with UTI in children with proven UTI.

Methods:
The serum levels of Zinc, vitamins A and D were compared between 25 proven UTI cases (admitted in 2 educational hospitals in Tehran) and 40 controls without infection (children who had undergone for elective surgery). The average age of children was 2.17 years. Atomic absorption Spectrophotometry, Radioimmunoassay, and HPLC methods were used for measuring the Zinc, Vit D and A, respectively.

Results:
Although the serum levels of zinc were significantly lower in UTI cases (P=0.05), no significant differences had observed between cases and controls for vitamins A and D in sera (P=0.4 and P=0.9).

Conclusion:
Due to established lower zinc level in UTI cases (p-value = 0.05), zinc deficiency might have a role in susceptibility to UTI in studied children. Administration of zinc could be helpful in preventing UTI. To establish the role of Vitamins A and D in vulnerability to UTI, further extensive research with larger samples is needed.

Conclusion:
Due to established lower zinc level in UTI cases (p-value = 0.05), zinc deficiency might have a role in susceptibility to UTI in studied children. Administration of zinc could be helpful in preventing UTI. To establish the role of Vitamins A and D in vulnerability to UTI, further extensive research with larger samples is needed.
THE DATA OF THE DARMA IN THE HISTORIOGRAPHIC PERSPECTIVE
Authors: Wijayanti Yeni 
Published: August 2019

AbstrakNaskah Sewaka Darma merupakan produk historiografi masyarakat Sunda masa lampau yang menggambarkan pandangan masyarakat kala itu. Kita bisa memahami kehidupan beragama dan pola perilaku masyarakat penganutnya. Akan tetapi, bagaimana sebenarnya Naskah Sewaka Darma dalam perspektif historiografi? Tulisan ini akan mengkaji pertanyaan tersebut. Metode yang digunakan adalah metode sejarah, yang meliputi heuristik, kritik, interpretasi, dan historiografi. Hasil kajian menunjukkan bahwa Naskah Sewaka Darma yang berisi tentang ajaran agama, khususnya uraian pelepasan moksa, adalah bagian dari historiografi tradisional dengan ciri-ciri tidak kronologis, religius, dan diliputi oleh kosmologis dan astrologis.Kata Kunci: Naskah, Sewaka Darma, Historiografi AbstractSewaka Darma Manuscript is a Sundanese product historiography of the past that depicts people's views at that time. We can understand the religious life and behavior patterns of the adherents. However, how exactly is the Sewaka Darma Manuscript in the perspective of historiography? This paper will examine the question. The method used is the historical method, which includes heuristics, criticism, interpretation, and historiography. The results of the study show that the Sewaka Darma Manuscript which contains religious teachings, specifically the description of the release of moksa, is part of traditional historiography with features not chronological, religious, and covered by cosmologists and astrologists.Keywords: Manuscripts, Sewaka Darma, Historiography
NILAI-NILAI KEARIFAN LOKAL DALAM TRADISI MISALIN DI KECAMATAN CIMARAGAS KABUPATEN CIAMIS
Authors: Ratih Dewi 
Published: August 2019

AbstrakTradisi Misalin merupakan upacara adat yang dilaksanakan secara rutin setiap setahun sekali di Desa Cimaragas menjelang bulan suci Ramadhan. Banyak masyarakat yang mengetahui dan bahkan mengikuti tradisi tersebut, tetapi belum menyadari penuh nilai-nilai kearifan lokal yang terkadung di dalamnya. Oleh karenanya, tulisan ini bertujuan untuk mengetahui pelaksanaan dan nilai-nilai kearifan lokal Tradisi Misalin di Desa Cimaragas. Metode yang digunakan adalah metode historis, yang terdiri dari heuristik, kritik, interpretasi, dan historiografi. Hasil penelitian menunjukkan bahwa Tradisi Misalin memiliki nilai-nilai kearifan lokal, di antaranya nilai religius, gotong royong, seni, sejarah, dan ekonomi.Kata kunci: Tradisi, Misalin, Kearifan Lokal AbstractThe Misalin tradition is a traditional ceremony that is routinely held every time it is held in the village of Cimaragas which asks for the holy month of Ramadan. Many people understand and even follow the tradition, but have not yet fully realized the local values contained in it. Therefore, this paper will discuss the implementation and values of the local wisdom of the Misalin Tradition in Cimaragas Village. The method used is the historical method, which consists of heuristics, criticism, interpretation, and historiography. The results of the study indicate that Misalin Tradition has local wisdom values, in religious exchange rates, mutual cooperation, art, history, and economics.Keywords: Tradition, Misalin, Local Wisdom
THE COMPETENCE OF HISTORY TEACHER IN IMPLEMENTING THE AUTHENTIC ASSESSMENT : A CASE STUDY AT SMA NEGERI 2 YOGYAKARTA
Authors: Aman Aman 
Published: August 2019

ABSTRAKPenelitian ini bertujuan mengetahui bagaimana: 1) realitas sistem penilaian pembelajaran sejarah di SMA Negeri 2 Yogyakarta selama ini, 2) kemampuan guru sejarah dalam mengembangkan instrumen penilaian otentik, 3) kompetensi guru sejarah dalam implementasi penilaian otentik, dan 4) kemampuan guru sejarah dalam mengolah hasil pengukuran. Hasil penelitian menunjukkan bahwa: 1) realitas sistem penilaian mengikuti rambu-rambu penilaian dalam Kurikulum 2013; 2) guru sejarah cukup mampu dalam mengembangkan instrumen penilaian otentik dibuktikan dengan instrumen penilaian yang dikembangkan sesuai dengan tuntutan kompetensi inti dan kompetensi dasar, 3) kemampuan guru sejarah dalam implementasi penilaian otentik cukup baik di mana ada kesesuaian antara instrumen yang dikembangkan dengan penerapannya dalam penilaian, dan 4) kemampuan guru sejarah dalam pengolahan hasil pengukuran cukup baik terbukti dengan dianalisisnya seluruh hasil pengukuran untuk dilakukan penilaian dan evaluasi. Simpulan penelitian ini, guru sejarah SMA Negeri 2 Yogyakarta mampu menerapkan penilaian otentik. Kata kunci: kompetensi, guru sejarah, dan penilaian otentik. ABSTRACTThe aims of this study were to find out: 1) the assessment system reality of teaching and learning of history subject at SMA Negeri 2 Yogyakarta; 2) the competence of history teacher in developing the instrument of authentic assessment; 3) the competence of history teacher in implementing authentic assessment; and 4) the competence of history teacher in processing the assessment result. The result of the study showed that: 1) the assessment system reality has followed the assessment guidance of Curriculum 2013; 2) the competence of history teacher in developing authentic assessment instrument has improved; 3) the competence of history teacher competence was good in implementing authentic assessment where there have been compatibility between developed instrument and assessment implementation; and 4) the competence of history teacher in processing the measurement result was good. The conclusion of this research, the teacher of SMA Negeri 2 Yogyakarta is competence in implementing authentic assessment. Key words: competence, history teacher, and authentic assessment.