bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of a three‐miRNA signature as a novel potential prognostic biomarker in patients with clear cell renal cell carcinoma
Authors: Luo Yongwen , Chen Liang , Wang Gang , Xiao Yu , Ju Lingao , Wang Xinghuan 
Published: April 2019

AbstractCurrent studies suggest that some microRNAs (miRNAs) are associated with prognosis in clear cell renal cell carcinoma (ccRCC). In this paper, we aimed to identify a miRNAs signature to improve prognostic prediction for ccRCC patients. Using ccRCC RNA‐Seq data of The Cancer Genome Atlas (TCGA) database, we identified 177 differentially expressed miRNAs between ccRCC and paracancerous tissue. Then all the ccRCC tumor samples were divided into training set and validation set randomly. Three‐miRNA signature including miR130b, miR‐18a, and miR‐223 were constructed by the least absolute shrinkage and selection operator (LASSO) Cox regression model in training set. According to optimal cut‐off value of three‐miRNA signature risk score, all the patients could be classified into high‐risk group and low‐risk group significantly. Survival of patients was significantly different between two groups (hazard ratio, 5.58, 95% confidence interval, 3.17‐9.80; P < 0.0001), and three‐miRNA signature performed favorably prognostic and predictive accuracy. The results were further validated in the validation set and total set. Multivariate Cox regression analyses and subgroup analyses showed that three‐miRNA signature was an independent prognostic factor. Two nomograms that integrated three‐miRNA signature and three clinicopathological risk factors were constructed to predict overall survival and disease‐free survival after surgery for ccRCC patients. Functional enrichment analysis showed the possible roles of three‐miRNA signature in some cancer‐associated biological processes and pathways. In conclusion, we developed a novel three‐miRNA signature that performed reliable prognostic for patient survival with ccRCC, it might facilitate ccRCC patients counseling and individualize management.
Loss of p27kip1 suppresses the myocardial senescence caused by estrogen deficiency
Authors: Yan Yi , Miao Dengshun , Yang Zhijian , Zhang Dingguo 
Published: April 2019

AbstractEstrogen deficiency accelerates the aging process and increases the risk of developing cardiovascular disease (CVD). Apoptosis is one of the important mechanisms of aging. p27kip1 is a cyclin‐dependent kinase inhibitor that can regulate cell cycle, apoptosis, and cell motility. p27kip1 overexpression can inhibit cell cycle and increase apoptosis so it has been considered as a marker of aging. In the present study, bilateral ovariectomy (OVX) was performed as a model for menopause in wild‐type (WT) and p27kip1 knockout (KO) mice to assess the effects of p27kip1 loss in myocardial aging caused by estrogen deficiency. We found that myocardial fibrosis and heart weight/body weight ratio of mice in the OVX group and p27kip1 KO group were significantly increased. Echocardiography showed that the left ventricular diameter and volume of the WT OVX group increased significantly and the cardiac function decreased. However, there was no significant difference in the results of echocardiography between the two p27kip1 KO groups. The aging and apoptosis indexes in OVX group were increased significantly, However, the indexes in p27kip1 KO mice were decreased. The expression of antioxidant indexes in OVX group was decreased significantly and p27kip1 KO can improve the antioxidant ability. These results provided that estrogen deficiency increased oxidative stress and apoptosis, accelerated aging of heart. p27kip1 KO can partly delay the aging and apoptosis of heart through upregulated antioxidant enzymes.
Suppression of c‐Myc using 10058‐F4 exerts caspase‐3‐dependent apoptosis and intensifies the antileukemic effect of vincristine in pre‐B acute lymphoblastic leukemia cells
Authors: Sheikh‐Zeineddini Negar , Bashash Davood , Safaroghli‐Azar Ava , Riyahi Niknam , Shabestari Rima Manafi , Janzamin Ehsan , Safa Majid 
Published: April 2019

AbstractDespite an old history behind the identification of the leading role of c‐Myc in leukemogenesis, the road to constructing a therapeutic perspective for this molecule in acute lymphoblastic leukemia (ALL) is yet mesmerizing. This study was designed to provide a better outlook for the anticancer property of 10058‐F4, an appealing inhibitor of c‐Myc, in pre‐B ALL cell lines either in the context of monotherapy or in combination with chemotherapeutic drugs. Our results declared that abrogation of c‐Myc decreased the proliferative capacity of pre‐B ALL‐derived cells through halting the transition of the cells from G1 phase, and reducing the replicative potential of both REH and Nalm‐6 cells, at least partly, through c‐Myc‐mediated suppression of human telomerase reverse transcriptase. Moreover, 10058‐F4 potently induced a caspase‐3‐dependent apoptosis in pre‐B ALL cells via shifting the balance between pro‐ and anti‐apoptotic target genes. Although the inhibition of PI3Kδ using Idelalisib upregulated the messenger RNA expression of autophagy‐related genes in 10058‐F4‐treated cells, treatment with autophagy inhibitor chloroquine decreased viability of the cells, either as a single agent or in combination with Idelalisib and/or 10058‐F4; suggesting that the activation of autophagy in pre‐B ALL cells could blunt apoptotic events and attenuate anticancer effect of both c‐Myc and PI3K inhibitors. Finally, the results of our synergistic experiments delineated that 10058‐F4 produced a synergistic effect with vincristine and provided an enhanced therapeutic efficacy in ALL cells, highlighting that c‐Myc oncoprotein could be a bona fide target for the treatment of ALL.
Lentiviral delivery of a shRNA sequence analogous to miR‐4319/miR‐125‐5p induces apoptosis in NSCLC cells by arresting G2/M phase
Authors: Xu Xuebo , Lai Yueyang , Zhou Wenzhao , Hua Zichun 
Published: April 2019

AbstractIn this study, we explored the therapeutic potential of microRNA (miR) analogs against non–small‐cell lung cancer (NSCLC) using lentiviral delivery of short hairpin RNA (shRNA). By using A549 as a model cell line, we used analogs and mimics of miR‐4319/miR‐125‐5p to target the tumorigenic RAF1 gene. Lentiviral vectors carrying shRNA of a highly efficient miRNA analog of miR‐4319/miR‐125‐5p, Analog2, were constructed to infect A549 cells. Our results showed that, compared with the noncancerous bronchial epithelial cell line 16HBE, lentivirus delivering Analog2 shRNA induced significant G2/M arrest and subsequent apoptosis in A549 cells, but not in 16HBE cells. Western blot analysis revealed that key factors regulating cell cycle were downregulated following RAF1 inhibition. In vivo xenograft experiments showed that lentivirus carrying Analog2 shRNA markedly decreased tumor size. Therefore, lentiviral delivery of Analog2 shRNA is a valid RNA interference‐based treatment against NSCLC with high potency and specificity.
Covering arrays for some equivalence classes of words
Authors: Cassels Joshua , Godbole Anant 
Published: April 2019

AbstractCovering arrays for words of length over a ‐letter alphabet are arrays with entries from the alphabet so that for each choice of columns, each of the ‐letter words appears at least once among the rows of the selected columns. We study two schemes in which all words are not considered to be different. In the first case known as partitioning hash families, words are equivalent if they induce the same partition of a element set. In the second case, words of the same weight are equivalent. In both cases, we produce logarithmic upper bounds on the minimum size of a covering array. Definitive results for , as well as general results, are provided.
Integrated mRNA and miRNA expression profile analyses reveal the potential roles of sex‐biased miRNA–mRNA pairs in gonad tissues of the Chinese concave‐eared torrent frog (Odorrana tormota)
Authors: Shu Yilin , Zhang Huijuan , Cai Qijia , Tang Dong , Wang Gang , Liu Ting , Lv Bihua , Wu Hailong 
Published: April 2019

AbstractThe Chinese concave‐eared torrent frog (Odorrana tormota) is typically sexually dimorphic. Females are significantly less common than males in the wild. Until now, the molecular mechanisms of reproduction and sex differentiation of frogs remain unclear. Here, we integrated mRNA and microRNA (miRNA) expression profiles to reveal the molecular mechanisms of reproduction and sex differentiation in O. tormota. We identified 234 differentially expressed miRNAs (DEMs) and 18,551 differentially expressed transcripts. Of these, 12,053 mRNAs and 64 miRNAs were upregulated in testes, and 6,498 mRNAs and 170 miRNAs were upregulated in ovaries. Integrated analysis of the miRNA and mRNA expression profiles predicted 75,602 potential miRNA–mRNA interaction sites, with 42,065 negative miRNA–mRNA interactions. We found 36 differentially expressed genes (DEGs) related to reproduction and sex differentiation, of which 15 DEGs formed 92 negative miRNA–mRNA interactions with 34 known DEMs. Thus, miRNAs may play other important roles in O. tormota. Furthermore, Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed reproductive‐related processes, such as the gonadotropinreleasing hormone signaling pathway and ovarian steroidogenesis. Based on functional annotation and the literature, the retinoic acid signaling pathway, the SOX9–AMH pathway, and the process of spermatogenesis may be involved in the molecular mechanisms of reproduction and sex differentiation in O. tormota, and may be regulated by miRNAs. The miRNA–mRNA pairs described may provide further understanding of the regulatory mechanisms associated with reproduction and sex differentiation, and the molecular mechanism of reproduction in O. tormota.
Access to sexual and reproductive health services for women living with HIV in Latin America and the Caribbean: systematic review of the literature
Authors: Gómez‐Suárez Marcela , Mello Maeve B , Gonzalez Mónica Alonso , Ghidinelli Massimo , Pérez Freddy 
Published: April 2019

AbstractIntroductionSystematic reviews show that women living with HIV (WLHIV) have high unmet sexual and reproductive health (SRH) needs due to barriers to access sexual and reproductive health services (SRHS). In Latin America and the Caribbean (LAC), as of 2016, there were nearly one million WLHIV, but the existing evidence of their SRH needs comes from a few individual studies. This systematic review provides an overview of these women's needs to help define new and/or adapt existing public health strategies to the local context. This review synthesizes the evidence from the literature on the use of and access to SRHS related to family planning, antenatal care, abortion services and violence against WLHIV in LAC.MethodsUsing a systematic review of mixed studies, a search was performed in MEDLINE, EMBASE, LILACS, INASP, POPLINE, SCOPUS, for studies conducted in LAC, from 2004 to 2017, as well as contact with authors and hand search as needed. Two independent reviewers evaluated the quality of the studies using the Mixed Methods Appraisal Tool; inclusion was conducted according to the PRISMA flow diagram. An exploratory narrative synthesis followed by quantitative synthesis data was undertaken. Group analysis or meta‐analysis was not considered appropriate given the level of heterogeneity of the studies.ResultsA total of 18 studies in 13 LAC countries for a population of 5672 WLHIV were included. Data from individual studies reported unmet family planning needs; higher, but inconsistent use of condom as the sole contraceptive method OR=1.46 [1.26 to 1.69]; lesser use of other non‐permanent contraceptive methods OR=0.26 [0.22 to 0.31]; more unplanned pregnancies OR=1.30 [1.02 to 1.66]; more induced abortions OR=1.96 [1.60 to 2.39]; higher risk of immediate postpartum sterilization; and higher exposure to sexual and institutional violence by WLHIV when compared with women without HIV.ConclusionsThis review presents evidence from LAC about the SRH unmet needs of WLHIV that must be addressed by decreasing institutional and structural barriers, facilitating services and reducing stigma, and discrimination among healthcare providers to improve access to SRHS based on human rights, so women independently of their HIV status can make their own reproductive decisions, free of violence and coercion.
Myeloid C-type lectin receptors in skin/mucoepithelial diseases and tumors
Authors: Tang Ce , Makusheva Yulia , Sun Haiyang , Han Wei , Iwakura Yoichiro 
Published: April 2019

Abstract
Myeloid C-type lectin receptors (CLRs), which consist of an extracellular carbohydrate recognition domain and intracellular signal transducing motif such as the immunoreceptor tyrosine-based activation motif (ITAM) or immunoreceptor tyrosine-based inhibitory motif (ITIM), are innate immune receptors primarily expressed on myeloid lineage cells such as dendritic cells (DCs) and Mϕs. CLRs play important roles in host defense against infection by fungi and bacteria by recognizing specific carbohydrate components of these pathogens. However, these immune receptors also make important contributions to immune homeostasis of mucosa and skin in mammals by recognizing components of microbiota, as well as by recognizing self-components such as alarmins from dead cells and noncanonical non-carbohydrate ligands. CLR deficiency not only induces hypersensitivity to infection, but also causes dysregulation of muco-cutaneous immune homeostasis, resulting in the development of allergy, inflammation, autoimmunity, and tumors. In this review, we introduce recent discoveries regarding the roles of myeloid CLRs in the immune system exposed to the environment, and discuss the roles of these lectin receptors in the development of colitis, asthma, psoriasis, atopic dermatitis, and cancer. Although some CLRs are suggested to be involved in the development of these diseases, the function of CLRs and their ligands still largely remain to be elucidated.
“Keep your feet on the ground”: Simulated range of motion and hind foot posture of the Middle Jurassic sauropodRhoetosaurus browneiand its implications for sauropod biology
Authors: Jannel Andréas , Nair Jay P. , Panagiotopoulou Olga , Romilio Anthony , Salisbury Steven W. 
Published: April 2019

AbstractThe biomechanics of the sauropod dinosaur pes is poorly understood, particularly among the earliest members of the group. To date, reasonably complete and articulated pedes in Early Middle Jurassic sauropods are rare, limited to a handful of taxa. Of these,Rhoetosaurus brownei, from eastern Australia, is currently the only one from the Gondwanan Middle Jurassic that preserves an articulated pes. UsingRhoetosaurus browneias a case exemplar, we assessed its paleobiomechanical capabilities and pedal posture. Physical and virtual manipulations of the pedal elements were undertaken to evaluate the range of motion between the pedal joints, under both bone‐to‐bone and cartilaginous scenarios. Using the results as constraints, virtual reconstructions of all possible pedal postures were generated. We show thatRhoetosaurus browneiwas capable of significant digital mobility at the osteological metatarsophalangeal and distal interphalangeal joints. We assume these movements would have been restricted by soft tissue in life but that their presence would have helped in the support of the animal. Further insights based on anatomy and theoretical mechanical constraints restricted the skeletal postures to a range encompassing digitigrade to subunguligrade stances. The approach was extended to additional sauropodomorph pedes, and some validation was provided via the bone data of an African elephant pes. Based on the resulting pedal configurations, the in‐life plantar surface of the sauropod pes is inferred to extend caudally from the digits, with a soft tissue pad supporting the elevated metatarsus. The plantar pad is inferred to play a role in the reduction of biomechanical stresses, and to aid in support and locomotion. A pedal pad may have been a key biomechanical innovation in early sauropods, ultimately resulting in a functionally plantigrade pes, which may have arisen during the Early to Middle Jurassic. Further mechanical studies are ultimately required to permit validation of this long‐standing hypothesis.
Multi‐Year Effect of Human Papillomavirus Vaccination on Recurrent Respiratory Papillomatosis
Authors: Matsuzaki Hiroumi , Makiyama Kiyoshi , Hirai Ryoji , Suzuki Hirotaka , Asai Ryohei , Oshima Takeshi 
Published: April 2019

ObjectiveTo examine whether human papilloma virus (HPV) vaccination in combination with surgical resection could suppress recurrence for an extended period of time in patients with recurrent respiratory papillomatosis (RRP).MethodsIn a prospective case series, data of patients who received combination therapy comprising surgery and quadrivalent HPV vaccination (Gardasil; Merck & Co., West Point, PA) were collected. Patients were followed up for RRP from March 2012 to July 2018 in an academic tertiary care center. The patients comprised 16 adults with RRP who were observed for >12 months after HPV vaccination, and whose HPV‐DNA status was tested before and for >12 months after completion of combination therapy. The outcomes of this study were the severity score of larynx disease, tumor incidence rate, and relationship between this rate and HPV‐DNA negative conversion after therapy.ResultsThe severity score of laryngeal disease significantly decreased from before combination therapy to the time of final examination (P = 0.00045). The tumor incidence rate decreased to approximately 20% during the period from 12 to 47 months after HPV vaccination. Regarding HPV‐DNA status in the final test results of each patient, 12 of 16 (75%) patients showed negative conversion; these 12 were significantly less likely to experience recurrence than patients who persistently tested positive for HPV‐DNA during the period between 12 and 47 months after vaccination.ConclusionHPV vaccination prevented recurrence associated with RRP surgery for 4 years in 80% of patients. This may be an effective adjuvant therapy, and HPV‐DNA negative conversion after HPV vaccination might predict prevention of recurrence.Level of Evidence2 Laryngoscope, 130:442–447, 2020
Effect of the T6 heat treatment on corrosion behavior of additive manufactured and gravity cast AlSi10Mg alloy
Authors: Girelli Luca , Tocci Marialaura , Conte Manuela , Giovanardi Roberto , Veronesi Paolo , Gelfi Marcello , Pola Annalisa 
Published: April 2019

AbstractThis study investigates the corrosion behavior of AlSi10Mg alloy produced by additive manufacturing and gravity casting before and after T6 heat treatment. Electrochemical tests showed that the additive manufactured material in as‐produced condition exhibits high corrosion resistance, due to the very fine microstructure, while immersion test allowed to identify an exfoliation‐like corrosion phenomenon, which caused a significant mass loss. In this case, T6 heat treatment was beneficial for corrosion resistance since the related change in microstructure led to a less detrimental corrosion mechanism. Therefore, the T6 heat treatment resulted necessary to use additive manufactured AlSi10Mg components in aggressive chloride‐bearing environments.
Neurorehabilitation Improves the Motor Features in Prodromal SCA2: A Randomized, Controlled Trial
Authors: Velázquez‐Pérez Luis , Rodríguez‐Diaz Julio C. , Rodríguez‐Labrada Roberto , Medrano‐Montero Jacqueline , Aguilera Cruz Annety B. , Reynaldo‐Cejas Lorenzo , Góngora‐Marrero Mariela , Estupiñán‐Rodríguez Annelié , Vázquez‐Mojena Yaimeé , Torres‐Vega Reidenis 
Published: April 2019

AbstractBackgroundThe search for early interventions is a novel approach in spinocerebellar ataxias, but there are few studies supporting this notion. This article aimed to assess the efficacy of neurorehabilitation treatment in prodromal spinocerebellar ataxia type 2.MethodsThirty spinocerebellar ataxia type 2 preclinical carriers were enrolled in a randomized, controlled trial using neurorehabilitation. The intervention in the treated group was 4 hours per day, 5 days per week for 12 weeks, emphasizing static balance, gait, and limb coordination. The control group did not receive rehabilitation. The primary outcome measure was the time for 5‐m tandem gait over the floor. Secondary outcomes included other timed tests with increased motor complexity, as well as the scores of the SARA and the Inventory of Non‐ataxia Symptoms.ResultsThe times for 5‐m tandem gait over the floor and the mattress were significantly reduced only in the rehabilitated group. Moreover, the times upholding the tandem stance over a mattress and the seesaw were notably increased only in this group. Likewise, the finger‐nose and the heel‐shin tests were improved in the rehabilitated group alone. The SARA score and the count of nonataxia symptoms were unchanged.ConclusionsThis rehabilitation program improves the subtle gait, postural and coordinative deficits in prodromal spinocerebellar ataxia type 2, which provided novel hints about the preservation of motor learning and neural plasticity mechanisms in early disease stages, leading chances for other interventional approaches in this and other spinocerebellar ataxias. © 2019 International Parkinson and Movement Disorder Society
Evaluation of short folded dipole antennas as receive elements of ultra‐high‐field human head array
Authors: Avdievich Nikolai I. , Solomakha Georgiy , Ruhm Loreen , Scheffler Klaus , Henning Anke 
Published: April 2019

PurposeTo improve the receive (Rx) performance of a human head transceiver (TxRx) array at 9.4T without compromising its transmit (Tx) performance, a novel 16‐element array was developed, constructed, and tested.MethodsWe designed and constructed a phased array, which consists of 8 TxRx surface loops placed in a single row and circumscribing a head, and 8 Rx‐only short folded dipole antennas. Dipoles were positioned along the central axis of each transceiver loop perpendicular to its surface. We evaluated the effect of Rx dipoles on the Tx efficiency of the array and maximum local specific absorption rate (SAR) as compared to the array of 8 surface loops only. We also compared the new array to a 16‐channel array of the same size consisting of 8 TxRx surface loops and 8 Rx‐only vertical loops in terms of Tx efficiency, SAR, and signal‐to‐noise ratio (SNR).ResultsThe new array improves both peripheral (up to 2 times) and central (1.17 times) SNR as compared to the 16‐element array of the same geometry consisting of 8 TxRx surface loops and 8 Rx‐only vertical loops. We demonstrated that an addition of actively detuned Rx‐only dipole elements produces only a small decrease (~7%) of the transmit field and a small increase (<7%) of the maximum local SAR.ConclusionAs a proof of concept, we developed and constructed a prototype of a 9.4T (400 MHz) head array consisting of 8 TxRx surface loops and 8 Rx‐only short optimized folded dipoles. We demonstrated that at ultra‐high field, dipoles outperformed Rx‐only vertical loops in vivo.