bluuebunny/crossref_metadata_2025_split huggingface dataset:
Separating Intervention from Regime Change: China's Diplomatic Innovations at the UN Security Council Regarding the Syria Crisis
Authors: Fung Courtney J. 
Published: June 2018

AbstractChina's response to the recent Syria crisis at the UN Security Council represents a crucial case in China's approach to intervention in that it breaks from China's recent practice of becoming more permissive regarding intervention. Instead, China actively worked to ensure that a firm line was drawn to separate intervention from foreign-imposed regime change. It did so by employing three diplomatic innovations: exercising multiple, successive vetoes; expanding discourse to delegitimize intervention as “regime change” by Western powers; and engaging in norm-shaping of the international community's “responsibility to protect” post-intervention. Together, these three innovations highlight China's desire to firmly separate the intervention norm from that of regime change. Using a variety of primary sources, the article also draws insights from interviews with foreign policy elites in Beijing, New York and New Delhi.
Lakes on the Tibetan Plateau as Conduits of Greenhouse Gases to the Atmosphere
Authors: Yan Fangping , Sillanpää Mika , Kang Shichang , Aho Kelly Sue , Qu Bin , Wei Da , Li Xiaofei , Li Chaoliu , Raymond Peter A. 
Published: June 2018

AbstractLakes play an important role in the global carbon cycle, and littoral zones of lakes are potential hotspots of greenhouse gas production. In this study, we measured the partial pressures of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) in the littoral zones of 17 lakes on the Tibetan Plateau. The littoral zones of lakes on the Tibetan Plateau were supersaturated and acted as sources of CO2, CH4, and N2O to the atmosphere. The average partial pressures of CO2, CH4, and N2O in the surface lake water were 664.8 ± 182.5, 139.8 ± 335.6, and 0.3 ± 0.1 μatm, respectively. The average diffusive fluxes (and uncentainty intervals) of these three gases were 73.7 (0.9–295.3) mmol · m−2 · day−1, 5.2 (0.0008–45.9) mmol · m−2 · day−1, and 6.5 (0.07–20.9) μmol · m−2 · day−1, respectively. The diffusive fluxes of CO2 in lakes were significantly correlated with dissolved organic carbon, dissolved organic nitrogen, salinity, and water temperature. The diffusive fluxes of N2O were significantly correlated with lake water depth. However, no relationships were found between environmental factors and the CH4 diffusive flux at the scale of this study. CO2 exchange with the atmosphere from saline lakes was found to be higher than from freshwater lakes with equivalent CO2 concentrations by a factor of 2.5 due to chemical enhancement of the gas transfer velocity. Therefore, further study with enhanced spatiotemporal resolution and breadth is needed to better understand the important role played by lakes on the Tibetan Plateau in both regional and global carbon cycles.
Nutrient Limitation and the Stoichiometry of Nutrient Uptake in a Tropical Rain Forest Stream
Authors: Tromboni Flavia , Thomas Steven A. , Gücker Björn , Neres‐Lima Vinicius , Lourenço‐Amorim Christine , Moulton Timothy P. , Silva‐Junior Eduardo F. , Feijó‐Lima Rafael , Boëchat Iola G. , Zandonà Eugenia 
Published: June 2018

AbstractNutrient limitation assessment is important to understand stream ecosystem functioning. Aquatic primary producers are often limited by nitrogen, phosphorus, or both, as assessed by nutrient diffusing substrata (NDS), a common method for assessing nutrient limitation in streams. But little is known regarding how this method relates to patterns of nutrient uptake at the whole‐stream scale. We combined two techniques to examine nutrient limitation in a tropical stream. First, we conducted two NDS experiments using ammonium, nitrate, and phosphate alone and combined, to determine nutrient limitation at substrata scale over several weeks. Second, we conducted whole‐stream nutrient addition experiments over the course of a year, using nutrients alone and in combination, to test theoretical predictions about uptake characteristics of limiting and nonlimiting nutrients. NDS results consistently indicated N limitation. Ambient uptake length (SW‐amb) suggested either P limitation (shorter SW‐amb for P than N) or colimitation (similar SW‐amb for both nutrients). The relationship between N uptake and concentration when added alone or with P suggested P limitation, colimitation, or neither, depending on the date. We speculate that the different conclusions arise from differences in the spatial and temporal scale assessed by these techniques and the microbial processes involved, and the potential for physical processes influencing whole‐stream uptake estimates. We conclude that nutrient limitation is not as categorical as NDS results often imply, rather habitat, resource, and biotic diversity result in nutrient uptake rates that do not necessarily conform to predictions drawn from fine scale, process‐specific bioassays such as chlorophyll‐a accrual on NDS.
Brain-actuated functional electrical stimulation elicits lasting arm motor recovery after stroke
Authors: Biasiucci A. , Leeb R. , Iturrate I. , Perdikis S. , Al-Khodairy A. , Corbet T. , Schnider A. , Schmidlin T. , Zhang H. , Bassolino M. , Viceic D. , Vuadens P. , Guggisberg A. G. , Millán J. d. R. 
Published: June 2018

AbstractBrain-computer interfaces (BCI) are used in stroke rehabilitation to translate brain signals into intended movements of the paralyzed limb. However, the efficacy and mechanisms of BCI-based therapies remain unclear. Here we show that BCI coupled to functional electrical stimulation (FES) elicits significant, clinically relevant, and lasting motor recovery in chronic stroke survivors more effectively than sham FES. Such recovery is associated to quantitative signatures of functional neuroplasticity. BCI patients exhibit a significant functional recovery after the intervention, which remains 6–12 months after the end of therapy. Electroencephalography analysis pinpoints significant differences in favor of the BCI group, mainly consisting in an increase in functional connectivity between motor areas in the affected hemisphere. This increase is significantly correlated with functional improvement. Results illustrate how a BCI–FES therapy can drive significant functional recovery and purposeful plasticity thanks to contingent activation of body natural efferent and afferent pathways.
C-terminal truncation of IFN-γ inhibits proinflammatory macrophage responses and is deficient in autoimmune disease
Authors: Dufour Antoine , Bellac Caroline L. , Eckhard Ulrich , Solis Nestor , Klein Theo , Kappelhoff Reinhild , Fortelny Nikolaus , Jobin Parker , Rozmus Jacob , Mark Jennifer , Pavlidis Paul , Dive Vincent , Barbour Sean J. , Overall Christopher M. 
Published: June 2018

AbstractControlled macrophage differentiation and activation in the initiation and resolution of inflammation is crucial for averting progression to chronic inflammatory and autoimmune diseases. Here we show a negative feedback mechanism for proinflammatory IFN-γ activation of macrophages driven by macrophage-associated matrix metalloproteinase 12 (MMP12). Through C-terminal truncation of IFN-γ at 135Glu↓Leu136 the IFN-γ receptor-binding site was efficiently removed thereby reducing JAK-STAT1 signaling and IFN-γ activation of proinflammatory macrophages. In acute peritonitis this signature was absent inMmp12–/–mice and recapitulated inMmp12+/+mice treated with a MMP12-specific inhibitor. Similarly, loss-of-MMP12 increases IFN-γ–dependent proinflammatory markers and iNOS+/MHC class II+macrophage accumulation with worse lymphadenopathy, arthritic synovitis and lupus glomerulonephritis. In active human systemic lupus erythematosus, MMP12 levels were lower and IFN-γ higher compared to treated patients or healthy individuals. Hence, macrophage proteolytic truncation of IFN-γ attenuates classical activation of macrophages as a prelude for resolving inflammation.
Giant intrinsic circular dichroism of prolinol-derived squaraine thin films
Authors: Schulz Matthias , Zablocki Jennifer , Abdullaeva Oliya S. , Brück Stefanie , Balzer Frank , Lützen Arne , Arteaga Oriol , Schiek Manuela 
Published: June 2018

AbstractMolecular chirality and the inherently connected differential absorption of circular polarized light (CD) combined with semiconducting properties offers great potential for chiral opto-electronics. Here we discuss the temperature-controlled assembly of enantiopure prolinol functionalized squaraines with opposite handedness into intrinsically circular dichroic, molecular J-aggregates in spincasted thin films. By Mueller matrix spectroscopy we accurately probe an extraordinary high excitonic circular dichroism, which is not amplified by mesoscopic ordering effects. At maximum, CD values of 1000 mdeg/nm are reached and, after accounting for reflection losses related to the thin film nature, we obtain a film thickness independent dissymmetry factorg = 0.75. The large oscillator strength of the corresponding absorption within the deep-red spectral range translates into a negative real part of the dielectric function in the spectral vicinity of the exciton resonance. Thereby, we provide a new small molecular benchmark material for the development of organic thin film based chiroptics.
The relationship between metabolic syndrome and asthma in the elderly
Authors: Park Sangshin , Choi Nam-Kyong , Kim Seungsoo , Lee Chang-Hoon 
Published: June 2018

AbstractThe burden of asthma in the elderly is increasing, but the etiology of asthma in the elderly is not clearly understood. Recent studies have reported the epidemiological link between metabolic syndrome (MS) and asthma, but it has rarely been studied in the elderly. This study investigated the association between MS and asthma and the contribution of insulin resistance (IR) and systemic inflammation to this MS-asthma association in the elderly. Our study analyzed 4,060 elderly participants (≥65 years old) from a cross-sectional survey, the Korean National Health and Nutritional Examination Survey 2007–2012. Mediation analyses were performed to examine whether IR and systemic inflammation mediates the MS-asthma association. Participants with MS had significantly higher prevalence of asthma (adjusted odds ratio = 1.34; 95% confidence interval = 1.09–1.64), and those who had greater waist circumference and lower HDL-C were especially likely to have asthma. Participants with IR and systemic inflammation were associated with higher prevalence of asthma. Prevalence of IR and systemic inflammation were higher in participants with MS or with each MS component. The MS-asthma association was substantially mediated by IR and systemic inflammation. Our study showed a significant association between MS and asthma in the elderly. MS might affect asthma through both IR and systemic inflammation.
Targeting the association between telomere length and immuno-cellular bioenergetics in female patients with Major Depressive Disorder
Authors: Boeck Christina , Salinas-Manrique Juan , Calzia Enrico , Radermacher Peter , von Arnim Christine A. F. , Dietrich Detlef E. , Kolassa Iris-Tatjana , Karabatsiakis Alexander 
Published: June 2018

AbstractMajor Depressive Disorder (MDD) has been associated with telomere dysfunction and alterations in mitochondrial activity, which seem to be co-regulated in human cells. To investigate this co-regulation in MDD, we assessed telomere length (TL) in peripheral blood mononuclear cells (PBMC) and selected immune cell subsets by quantitative fluorescence in situ hybridization and mitochondrial respiratory activity in PBMC by high-resolution respirometry in a study cohort of 18 MDD patients and 21 non-depressed controls. We provide initial evidence for a differential vulnerability to telomere attrition in selected adaptive immune cell populations. Here we found the highest difference in TL between depressed and control subjects for memory cytotoxic T cells. Depression was associated with reduced mitochondrial activity (mitochondrial bioenergetics), but increased mitochondrial density (mitochondrial biogenesis) in PBMC. Exploratory post-hoc analyses indicated that the changes in TL and immune cell bioenergetics were most pronounced in MDD patients who reported experiences of childhood sexual abuse. Among MDD patients, PBMC TL was as a trend positively associated with mitochondrial density and negatively associated with mitochondrial leak respiration, but not with mitochondrial activity related to biological energy production. These initial findings support the hypothesis of a co-regulation between telomeres and mitochondrial biogenesis but not mitochondrial bioenergetics among MDD patients.
A novel vibration-induced exercise paradigm improves fitness and lipid metabolism of Caenorhabditis elegans
Authors: Teo Emelyne , Batchu Krishna Chaithanya , Barardo Diogo , Xiao Linfan , Cazenave-Gassiot Amaury , Tolwinski Nicholas , Wenk Markus , Halliwell Barry , Gruber Jan 
Published: June 2018

AbstractExercise has been known to reduce the risk of obesity and metabolic syndrome, but the mechanisms underlying many exercise benefits remain unclear. This is, in part, due to a lack of exercise paradigms in invertebrate model organisms that would allow rapid mechanistic studies to be conducted. Here we report a novel exercise paradigm in Caenorhabditis elegans (C. elegans) that can be implemented under standard laboratory conditions. Mechanical stimulus in the form of vibration was transduced to C. elegans grown on solid agar media using an acoustic actuator. One day post-exercise, the exercised animals showed greater physical fitness compared to the un-exercised controls. Despite having higher mitochondrial reactive oxygen species levels, no mitohormetic adaptations and lifespan extension were observed in the exercised animals. Nonetheless, exercised animals showed lower triacylglycerides (TAG) accumulation than the controls. Among the individual TAG species, the most significant changes were found in mono- and polyunsaturated fatty acid residues. Such alteration resulted in an overall lower double bond index and peroxidation index which measure susceptibility towards lipid peroxidation. These observations are consistent with findings from mammalian exercise literature, suggesting that exercise benefits are largely conserved across different animal models.
Avian influenza surveillance in domestic waterfowl and environment of live bird markets in Bangladesh, 2007–2012
Authors: Khan Salah Uddin , Gurley Emily S. , Gerloff Nancy , Rahman Md Z. , Simpson Natosha , Rahman Mustafizur , Haider Najmul , Chowdhury Sukanta , Balish Amanda , Zaman Rashid Uz , Nasreen Sharifa , Chandra Das Bidhan , Azziz-Baumgartner Eduardo , Sturm-Ramirez Katharine , Davis C. Todd , Donis Ruben O. , Luby Stephen P. 
Published: June 2018

AbstractAvian influenza viruses, including highly pathogenic strains, pose severe economic, animal and public health concerns. We implemented live bird market surveillance in Bangladesh to identify the subtypes of avian influenza A viruses in domestic waterfowl and market environments. We collected waterfowl samples monthly from 4 rural sites from 2007 to 2012 and environmental samples from 4 rural and 16 urban sites from 2009 to 2012. Samples were tested through real-time RT-PCR, virus culture, and sequencing to detect and characterize avian influenza A viruses. Among 4,308 waterfowl tested, 191 (4.4%) were positive for avian influenza A virus, including 74 (1.9%) avian influenza A/H5 subtype. The majority (99%, n = 73) of the influenza A/H5-positive samples were from healthy appearing waterfowl. Multiple subtypes, including H1N1, H1N3, H3N2, H3N6, H3N8, H4N1, H4N2, H4N6, H5N1 (clades 2.2.2, 2.3.2.1a, 2.3.4.2), H5N2, H6N1, H7N9, H9N2, H11N2 and H11N3, H11N6 were detected in waterfowl and environmental samples. Environmental samples tested positive for influenza A viruses throughout the year. Avian influenza viruses, including H5N1 and H9N2 subtypes were also identified in backyard and small-scale raised poultry. Live bird markets could be high-risk sites for harboring the viruses and have the potential to infect naive birds and humans exposed to them.
Further dissection of QTLs for salt-induced stroke and identification of candidate genes in the stroke-prone spontaneously hypertensive rat
Authors: Niiya Kaoru , Ohara Hiroki , Isono Masato , Sheikh Abdullah Md. , Matsuo Hiroyuki , Fujikawa Koichi , Isomura Minoru , Kato Norihiro , Nabika Toru 
Published: June 2018

AbstractWe previously revealed that two major quantitative trait loci (QTLs) for stroke latency of the stroke-prone spontaneously hypertensive rat (SHRSP) under salt-loading were located on chromosome (Chr) 1 and 18. Here, we attempted further dissection of the stroke-QTLs using multiple congenic strains between SHRSP and a stroke-resistant hypertensive rat (SHR). Cox hazard model among subcongenic strains harboring a chromosomal fragment of Chr-1 QTL region showed that the most promising region was a 2.1 Mbp fragment between D1Rat177 and D1Rat97. The QTL region on Chr 18 could not be narrowed down by the analysis, which may be due to multiple QTLs in this region. Nonsynonymous sequence variations were found in four genes (Cblc, Cxcl17, Cic, and Ceacam 19) on the 2.1 Mbp fragment of Chr-1 QTL by whole-genome sequence analysis of SHRSP/Izm and SHR/Izm. Significant changes in protein structure were predicted in CBL-C and CXCL17 using I-TASSER. Comprehensive gene expression analysis in the kidney with a cDNA microarray identified three candidate genes (LOC102548695 (Zinc finger protein 45-like, Zfp45L), Ethe1, and Cxcl17). In conclusion, we successfully narrowed down the QTL region on Chr 1, and identified six candidate genes in this region.
Consequences of in utero exposure to Zika virus in offspring of AG129 mice
Authors: Julander Justin G. , Siddharthan Venkatraman , Park Albert H. , Preston Elizabeth , Mathur Pranav , Bertolio Michael , Wang Hong , Zukor Katherine , Van Wettere Arnaud J. , Sinex Donal G. , Morrey John D. 
Published: June 2018

AbstractZika virus (ZIKV) can cause various diseases in offspring after congenital infection. The purpose of this study was to identify disease phenotypes in pups exposed to ZIKV in utero. Female interferon-α/β, -γ receptor knockout mice (AG129) were infected intraperitoneally with ZIKV 7.5 days’ post coitus (dpc). Viral RNA, antigen and infectious virus were detected in some, but not all, maternal and fetal tissues at various times during gestation. Fetuses of infected dams had significant intrauterine growth restriction (IUGR), which was more pronounced as females neared parturition. Pups born to infected dams were significantly smaller and had significantly shortened skull lengths, as determined by measurement with a caliper and by micro-CT analysis, as compared with age-matched controls. Growth rates of exposed pups after birth, however, was similar to sham-exposed offspring. Viral RNA was detected in pups of infected dams after birth. A lower survival rate was observed in neonates exposed to ZIKV in utero. A mortality rate of over 50%, attributed to consequences of ZIKV infection, occurred after birth in pups born to infected dams. A transient hearing loss was observed in some animals exposed to virus in utero. No motor deficits or cognitive deficits were detected using running wheel or viral paresis scoring assays. Abnormalities in offspring included smaller size, shorter skull length and increased neonatal mortality, while the only functional deficit we could detect was a low incidence of transient hearing loss.
Ion energy spectra directly measured in the interaction volume of intense laser pulses with clustered plasma
Authors: Bochkarev S. G. , Faenov A. , Pikuz T. , Brantov A. V. , Kovalev V. F. , Skobelev I. , Pikuz S. , Kodama R. , Popov K. I. , Bychenkov V. Yu. 
Published: June 2018

AbstractThe use of gas cluster media as a target for an intense femtosecond laser pulses is considered to be uniquely convenient approach for the development of a compact versatile pulsed source of ionizing radiation. Also, one may consider cluster media as a nanolab to investigate fundamental issues of intense optical fields interaction with sub-wavelength scale structures. However, conventional diagnostic methods fail to register highly charged ion states from a cluster plasma because of strong recombination in the ambient gas. In the paper we introduce high-resolution X-ray spectroscopy method allowing to study energy spectra of highly charged ions created in the area of most intense laser radiation. The emission of CO2 clusters were analyzed in experiments with 60 fs 780 nm laser pulses of 1018 W/cm2 intensity. Theory and according X-ray spectra modeling allows to reveal the energy spectra and yield of highly charged oxygen ions. It was found that while the laser of fundamental frequency creates commonly expected monotonic ion energy spectrum, frequency doubled laser radiation initiates energy spectra featuring of distinctive quasi-monoenergetic peaks. The later would provide definite advantage in further development of laser-plasma based compact ion accelerators.
Effect of calcium electroporation on tumour vasculature
Authors: Staresinic Barbara , Jesenko Tanja , Kamensek Urska , Krog Frandsen Stine , Sersa Gregor , Gehl Julie , Cemazar Maja 
Published: June 2018

AbstractCalcium electroporation (CaEP) is a novel anti-tumour treatment that induces cell death by internalization of large quantities of calcium. The anti-tumour effectiveness of CaEP has been demonstrated in vitro, in vivo, and in preliminary clinical trials; however, its effects on the vasculature have not been previously investigated. Using a dorsal window chamber tumour model, we observed that CaEP affected to the same degree normal and tumour blood vessels in vivo, as it disrupted the vessels and caused tumour eradication by necrosis. In all cases, the effect was more pronounced in small vessels, similar to electrochemotherapy (ECT) with bleomycin. In vitro studies in four different cell lines (the B16F1 melanoma, HUVEC endothelial, FADU squamous cell carcinoma, and CHO cell lines) confirmed that CaEP causes necrosis associated with acute and severe ATP depletion, a picture different from bleomycin with electroporation. Furthermore, CaEP considerably inhibited cell migratory capabilities of endothelial cells and their potential to form capillary-like structures. The finding that CaEP has anti-vascular effects and inhibits cell migration capabilities may contribute to the explanation of the high efficacy observed in preclinical and clinical studies.
Raman Spectroscopy detects changes in Bone Mineral Quality and Collagen Cross-linkage in Staphylococcus Infected Human Bone
Authors: Khalid Mohamed , Bora Tanujjal , Ghaithi Ahmed Al , Thukral Sharanjit , Dutta Joydeep 
Published: June 2018

AbstractDiagnosis of osteomyelitis presents a formidable challenge. Lack of pathognomonic clinical sign(s) and diagnostic tests that can diagnose osteomyelitis at an early stage contribute to this difficulty. If the diagnosis is not made early, the disease becomes very difficult to eradicate and can lead to limb threatening and potentially life-threatening complications. Staphylococcus aureus is the most common organism causing osteomyelitis. Raman Spectroscopy provides information about molecular vibration that could potentially be harnessed as a spectral signature for cellular changes in specific pathologic conditions. In this study we describe a technique using Raman spectroscopy that could potentially be used to diagnose staphylococcal osteomyelitis. Human bone samples were co-cultured with Staphylococcus aureus (S. aureus) and the effects of bacterial growth on bone quality were then monitored using Raman spectroscopy. A major drop in the bone mineral quality and crystallinity was observed in the infected bones compared to the controls. S. aureus infection was also found to alter the collagen cross-linking. Our study shows that specific spectral signatures are present for the cause as well as the effect of staphylococcal osteomyelitis, opening the possibility of developing a useful diagnostic modality for early and rapid diagnosis of this condition.