bluuebunny/crossref_metadata_2025_split huggingface dataset:
Propolis Potential Toward the Amount of Limphoblast and Spleen Diameter of Male Mice (Mus musculus)
Authors: Rinaldhi Werstant Adhityananda , Hestianah Eka Pramyrtha , Sosiawati Sri Mumpuni , Yustinasari Lita Rakhma 
Published: March 2018

The aim of this research was to find out the effect of propolis in the amount of lymphoblast and the correlation between the treatment of propolis with spleen diameter each of white male mice (Mus musculus). This research used 25 male white mices which are 12 weeks old has 25-35 gram of body weight. They were divided randomly into five groups. (P0) as control was given 0,5 ml aquades/day, and other group were given propolis for 0.4 mg/0.03kg/day (P1), 0.8 mg/0.03kg/day (P2), 1.6 mg/0.03kg/day (P3), and 3.2 mg/0.03kg/day (P4). After two weeks treatment, 25 mices were sacrificed their spleen were then used in histological preparation with HE staining. The data were analyzed by ANOVA method based on Completely Randomized Design and further analyzed by Duncan's Multiple Range Test. The result from statistical analysis showed that treatment with propolis increased the amount of lymphoblast also the spleen diameter of white male mice (p<0,05) and show positive correlation amount of lymphoblast and white pulp spleen diameter. Keyword: propolis, lymphoblast, white pulp, spleen
Identification the Gene Nucleotide Sequence of Outer Membrane Protein Aeromonas Hydrophilla Bacteria from East Java Local Isolates Using Polimerase Chain Reaction
Authors: Yuliani M. Gandul Atik , Handijatno Didik , Madyawati Sri Pantja 
Published: March 2018

Aeromoniasis disease or known Ulcer Diseases caused by Aeromonas hydrophila which in fish or shrimp often occurs in some embankment or in the Aquarium can cause sign patches – patches of bleeding can even cause economic loss and death if not promptly treated with treatment that accompanied the good sanitation. This disease affects many farmed freshwater fish in East Java. Previous research has managed to do the characterization of antigenic protein originating from the OMP (outer membrane protein) Aeromonas hydrophilla. Because it is felt necessary to do DNA sequensing encoder protein in question.To achieve these goals then do some exploratory research laboratoric methods, namely: identification of the bacterium Aeromonas hydrophilla fish from suspect infection by PCR, gel electrophoresis and Sequencing of gene fragments of the agarosa OMP A. hydrophilla isolates of East Java. The results obtained from this research is the result of agarosa gel electrophoresis in DNA chromosome Aeromonas hydrophila bacteria cells is carried out at a temperature of annealing 54oC position 400 bp and the results of the sequencing primer that is used produces a sequence of nucleotides is around 355 nucleotides. Key words: Aeromonas hydrophilla, agarosa gel electrophoresis, DNA sequencing
Postmortem Interval Estimation Time from Algormortis Temperature of Rats Expressed by MARS Model Approach
Authors: Syabani Dwi M. , Eliyani Hana , Suharsono Suharsono , Rantam Fedik A. , Ma’ruf Anwar 
Published: March 2018

Estimation of Postmortem is one of the challenges in forensic science. The aim of this study was to construct a MARS model of Postmortem interval estimation time (PMT) from algor mortis temperature in Rat. Sixteen healthy male rats (Rattus norvegicus), onemonth old and weigh 100 gram were randomly divided into two groups (eight/each group) and were acclimated respectively among the ambient room (temperature over 28ºC) and at the conditioning room (temperature over 20ºC). The animals then were sacrificed in two days (four rats/day for each divided room) then algor mortis by rectal temperature were recorded after death at 0 and 2,4,6,8, 10,12, 14,16, 18,20 till 22 h respectively. The MARS model is nonlinear regression but performed as a multilinier curve that can have splines fitting and be defined as function model Y = 35.321 + 1.253 * BF1 + 0.436 * BF2 - 1.319 * BF3; and on 20ºC condition room as Y = 29.980 + 1.354 * BF1 + 0.799 * BF2 - 1.347 * BF3. Therefore, performance model was comprised by multilinier curve, then function model of algor mortis on ambient room be defined into three PMT intervals i.e: 1)Y=37,94 -0.11*(0-2h) (p>0.00); 2) Y = 40.88 - 1.87* (2-6h) ( p<0.00) and 3) Y=30.82-0.09*(6-22h) (p<0.00) while on 20ºC condition room, was : 1)Y = 34.78-0.09* (0-2h) (p<0.00) ; 2) Y = 37.97-2.38* (2-6h) (p<0.00) and 3)Y = 25.36-0.04* 6-22 h (p>0.00). The acceleration of the declining algor mortis at conditioning room showed steeper than on ambient room at 2-6h PMT interval (ß : 2,38 vs 1,87). Postmortem Time Interval Estimation from Algormortis Temperature of Rats could be expressed by MARS Model. The pattern model of estimation comprised by multilinear curve with splines was fitted at both of the experimental rooms.Keywords : Postmortem time interval, algor mortis, MARS model estimation
Aquoeous Extract of Neem Leaves (Azadirachta Indica) Decrease Expression of Immunoglbulin E (IgE) and Interleukin 4 (IL-4) in Gingiva Tissue of BALB/c Mice Injected by Ovalbumine
Authors: Ratna Dewanti I Dewa Ayu , Susilawati I Dewa Ayu , Lestari Pujiana Endah , Budirahardjo Roedy , Wulandari Erawati , Widi Ristya , Wibisono Sunlip 
Published: March 2018

Allergy has been such a difficult disease to cure. Some anti-allergic drugs on the market caused side effects such as dryness of mucous membranes, confusion, dizziness, dry mouth, constipation, difficulty urinating and blurred vision, bull neck and moon face. It is difficult to find anti-allergic drugs with minimum side effects. Aim. The research aimed to find alternative anti-alergic agent, it was aquoeous extract of Neem leaves. In particular, this study analysed modulation of aquoeous extract of Neem leaves to expression of Ig E and IL-4 in gingival tissue. Materials and Methods. A total 15 BALB/c Mice was divided into three groups, i.e controle group (no treatment were introduced), Ova group (On days 0, 7, 14 submucous injected with ovalbumine), Neem group (were given with aquoeous extract of Neem leaves 200 mg/day/kg body wieght and injected with ova). Ova injection was 25 μL (in 2 mg/mL PBS), while neem leaves extract were given by oral sondation. IgE and IL-4 were analysed in gingival tissue on day 21 by imunohistochemistry method. Parameters are amouts of leucocytes that expressed IgE and IL-4. Results. ANOVA and LSD test showed that aquoeous extract of Neem leaves could decrease IgE and IL-4 expression significantly (p<0,05) in gingival tissue. Conclusion. Aquoeous extract of Neem leaves could be used as an alternative for anti-alergic agent. Key words : Aquoeous Extract from Neem Leaves ; Azadirachta Indica ; Ig E ; IL-4 ; ovalbumine ; oral
Experimental Models Point Mutations In Plasmodium falciparum pfatpase6 Gene Exposed to Recuring Artemisinin In Vitro
Authors: Maslachah Lilik , Dachlan Yoes Prijatna , Nidom Chairul A. , Fitr Loeki Enggar 
Published: March 2018

The aims of this research to prove that repeated exposure of artemisinin can cause pfatpase6 gene mutation on Plasmodium falciparum in vitro. The research methods used culture In Vitro Plasmodium falciparum of strain 2300 IC50 value determination test artemisinin, artemisinin repeated exposure test (PO1, PO2, PO3 dan PO4) dose IC50, DNA extraction, gene amplification of pfatpase6 using Polymerase Chain Reaction (PCR) technique, electrophoresis, PCR product purification, labeling DNA from PCR results, DNA precipitation of PCR product, application of product labeling on the sequencing machines, analysis of the results of sequencing, and Data Analysis. The results of PCR pfatpase6 gene amplification include region 6 – 3216 for codon 89-1031 located in exon 1 and 2 Plasmodium falciparum 2300 by using five pairs of primers. Primer pair 1FR produce a long amplicon of 737 bp which covers of codon 89; primer pair 2FR produce a long amplicon of 813 bp which covers of codon 263, 431; primer pair 4FR produce a long amplicon of 700 bp which covers of codon 460, 465, 623; primer pair 5FR produce a long amplicon of 550 bp which includes of codon 683, 769; and primer pair 6FR produce a long amplicon of 876 bp which covers of codon 898, 1031.Multialigment pfatpase6 gene Plasmodium falciparum of strains Papua 2300 point mutations are obtained in the form of transition and transversion in treatment groups at the same nucleotide region 123, 2035, 2043, 2138 dan 2148. Conclusion of this research Artemisinin repeated exposure can cause point mutations in pfatpase6 genes Plasmodium falciparum of strains 2300 in vitro Keyword: Artemisinin, Plasmodium falciparumof strain Papua 2300, pfatpase6 gene, point mutation
Sinensetin-Rich Fraction Solid Dispersion Inhibits Cancer Cell Cycle
Authors: Arifianti Lusiana , Sukardiman Sukardiman , Santosa Mulja Hadi 
Published: March 2018

Drug development efforts to find new selective and safe drugs for cancer from natural resources are promising ones. The natural products are obtained in the multiple or single compounds. One of them is a sinensetin found in ethanol extract of Orthosiphon stamineus Benth. Sinensetin could inhibit cancer cell proliferation. However, it has a poor solubility so the absorption is low and then it impacts on the low bioavailability. The solubility problem in conventional drug could be solved by pharmaceutical manipulation. In the previous research, the manipulation was tried although there was no single compound found in the material tested. We found an optimal formula of a manipulation using solid disperse system of polyethylene glycol (PEG 6000) 15 times higher than sinensetin weight. This research was focused on observing the effect of the optimal formula of solid disperse system to inhibit cancer cell cycle. The cell lines used were T47D cells. The method used was flow cytometry. The result showed that the optimum formula has a consistent effect on the concentration of 40 and 60µg/mL. The sinensetin increase cell accumulation on S phase at the percentage of 18.80% (40µg/mL) and 22.21% (60µg/mL) compared to T47D normal cells. It reflects the S phase as the longest time experienced by the cells. Inhibition on S phase (S arrest) resulted from a DNA elongation. It causes an inhibition of DNA synthesis process. It could be concluded that the solid disperse of sinensetin was active to inhibit cancer cells proliferation on phase S. Keywords: cell cycle; sinensetin; solid disperse; poor solubility
Blunted Expression of PPARa in Mice with FABP-4 and -5 Deficiency under Acute Cold Exposure
Authors: Syamsunarno Mas Rizky A.A , Putri Mirasari , Iso Tatsuya , Widyastuti Rini , Panigoro Ramdan , Kurabayashi Masahiko 
Published: March 2018

Brown Adipose Tissue (BAT) is a nonshivering thermogenesis organ during cold exposure. Peroxisomal proliferator activated receptor alpha (PPARa) is the member of the nuclear hormone receptor superfamily and primarily expressed in BAT and liver. PPARa is coordinated with uncoupling protein 1 (UCP1) to regulate fatty acid metabolism in BAT. Fatty acid binding protein (FABP)-4 and-5 play role in adaptive response under fasting and cold exposure. The purpose of this study was to investigate the expression of PPARa in mice with FABP4/5 deficiency (DKO). Wildtype (WT) and DKO mice were exposed to cold for 2 hours under fed or 20 hours-fasted conditions. BAT was collected and further mRNA level of PPARa was examined using quantitative real-time PCR. As the result, PPARa gene expression in WT mice were increased 50% and 100% in fed and fasted condition respectively after cold exposure. There was no alteration in PPARa expression in BAT of DKO mice. As conclusion, The functional FABP-4 and -5 are necessary to modulate PPARa gene expression in Brown Adipose Tissue under acute cold exposure Keywords: Acute cold exposure; FABP4; FABP5; Fasting PPARa
Low Serum Cholesterol in Mice Pre-treated with Imperata cylindrica L. after Acute Olive Oil Gavage
Authors: Anggraeni Neni , Syamsunarno Mas Rizky A.A , Mukarromah Ghina Rahmadianti , Zada Almira , Triatin Rima Destya , Pamela Yunisa , Dhianawaty Diah 
Published: March 2018

Imperata cylindrica L. extract was studied for its effect on cholesterol absorption rate in mice (Mus muscularis). Male mice (20-25g) were divided control and pre-treated group. Pretreated group was further divided into two groups that received different dose of Imperata cylindrica L. extract gavage (90mg/KgBW and 115mg/KgBW) for 2 weeks. Mice were fasted 12 hour before acutely given olive oil gavage with dosage of 10 µl/g, and blood was collected at 0, 2, and 6 hour-time points on the indicated day. Blood was collected from the retroorbital plexus at indicated time points. Total cholesterol level was significantly (p<0.05) decreased in both pretreated groups at 6 hours after acute olive oil gavage. In conclusion, there is a significant effect of Imperata cylindrica L. extract to reduce cholesterol level in serum, suggesting its potential effect as an antihypercholesterolemia therapy. Key words: Imperata cylindrical L.; cholesterol absorption; antihypercholesterolemia; lipid absorption; herbal medicine
The Role Of IL-6 In TMPD-Treated Lupus Arthritis Mice
Authors: Indriyanti Niken 
Published: March 2018

Lupus or systemic lupus erythematosus is a chronic autoimmune disease with systemic inflammation manifestations mainly in targeted organs. The appropriate animal model for lupus is necessary. The induction method by using 2,6,10,14 tetramethylpentadecane (TMPD) reveals more complex manifestations than other hydrocarbons. However, the autophagy of macrophages as an effect of TMPD makes differences to make the decision in lupus biomarker as a targeted therapy in lupus arthritis. Thus, this research focused on the role of CD68+IL-6 produced by macrophages and total IL-6 in lupus in correlation to the arthritis severity. The naïve and TMPD-treated groups (n=3) were induced by means of 0.5 ml TMPD i.p. After 6 months, the mice were sacrificed then the fresh spleens were prepared as isolated cells to be measured by using flow cytometry method. The knee joints were prepared for histology observation. The statistical analysis was performed by using T-test SPSS 22 version. The results showed the relative percentage of CD68+IL-6+ in the TMPD-treated group increased significantly (P<0.05) with the value of 62.38±9.97 %, compared to naïve group 49.70±2.34 %. Moreover, the total IL-6 did not increase significantly (P>0.05). Meanwhile, the arthritis severity score of the TMPD-treated group revealed severe erosion with the grade of 3.7±1.06, higher significantly (P<0.05) than the naïve group (0.5±0.71). The joint spaces in both groups were not significantly different. Finally, the observations gave the clear information that despite the autophagy potency, the CD68+IL-6 and the arthritis severity score were good markers in lupus preclinical study. Keywords: CD68+IL-6; inflammation; lupus arthritis; TMPD
Immunopathological Approach for Avian Influenza Virus Detection in Brain of Laying Bird with Clinical Signs of Torticollis and Curled Toe Paralysis
Authors: Yunita Niken , Wulan Ocie Harum , Wuryastuty Hastari , Wasito Raden 
Published: March 2018

Infection with avian influenza virus (AIV) in laying birds field cases often lead to clinical signs of torticollis and curled toe paralysis. Clinical signs of torticollis and curled toe paralysis is similar to the clinical signs of Newcastle disease virus infection in poultry, making it difficult for the confirmation of diagnosis of infection with both viruses. Immunopathological immunohistochemistry streptavidin-biotin (IHC SB) is an antibody-based test to detect the presence of pathogens, especially AIV in poultry with the principle of antigen detection in tissue specimens. This research aims to detect AIV infection in laying birds field case with clinical signs of torticollis and curled toe paralysis. The samples (brains from 20 layer chicken) were taken from the cases of the disease in poultry in several commercial poultry farms. The chickens showed clinical signs of torticollis and curled toe paralysis, and oedema in the brain suspected of being infected AIV. After being necropsied, then the brains were tested IHC SB and observed with a digital microscope camera system and analyzed descriptively qualitative. The results showed that the avian influenza virus antigens was found in the parenchyma, neural and endothelial cells of brain blood vessels. Based on these results, we can conclude that Immunopathologic immunohistochemical staining of streptavidin-biotin can be applied for confirmation of the diagnosis of avian influenza virus (AIV) on the brain of commercial laying chickens showing torticollis and curled toe paralysis. Keywords : AIV; IHC SB; brain; torticollis; curled toe paralysis
The Efficacy of Permot (Passiflora Foetida Linn.) Leaves Crude Extract Ointment on the Healing of Skin of Rabbit with Scabies
Authors: Hastutiek Poedji , Eliyani Hana 
Published: March 2018

This research aimed to know the efficacy of active compound of Permot (P. foetida) leaves crude extract ointment as a bioacaricide in rabbit through observation of after treatment histopathological changes on skin of body part naturally infested with Sarcoptes scabiei. Permot leaves were isolated and extracted in n-hexane solvent. Sixteen rabbits were randomly divided into four groups, control (P0), P1, P2 and P3, the group of rabbits with scabies treated respectively with 5, 10 and 15 % of permot leaves crude extract ointment. Therapy was conducted topically on day 0; 1; 2; 3; 4; 5. Efficacy was examined from Haematoxylin Eosin (HE) stained skin histopathological features under microscope with magnification of 100x, 400x and 1000x. The obtained skin histopatological feature data were assessed qualitatively. The application of 5 % ointment (P1) showed erosion and severe inflammatory cells infiltration in the epidermis; follicle proliferation and sebaceous were not present. In the dermis, severe sebaceous adenitis and folliculitis were evident. Massive inflammatory cells infiltration was found in hypodermis. The therapy using 5% ointment resulted 45 % success rate. The application of 10 % permot leaves crude extract ointment (P2) showed severe hypergranulation in the epidermis, severe proliferation of follicle and sebaceous gland, mild sebaceous adenitis and mild folliculitis in the dermis, as well as moderate inflammatory cells infiltration in the hypodermis. The therapy using 10,% ointment resulted 60 % success rate. The application of 15 % ointment (P3) showed mild hypergranulation in the epidermis, mild follicle proliferation and inflammatory cells (PMN) infiltrationin the dermis; and moderate inflammatory cells infiltration in the hypodermis. The therapy using 15 % ointment resulted 90 % success rate. Keywords: scabiosis, rabbits, Passiflora foetida, ointment, histopathology
The Effect of Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment on the Liver Structure of Iron overload of Rat (Rattus norvegicus)
Authors: Safitri Ratu , Reniarti Lelani , Madihah Madihah , Delia Lila , Syamsunarno Mas Rizky A.A , Panigoro Ramdan 
Published: March 2018

This research was carried out to detect the effect of Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment on the liver structure of iron overload rat (Rattus norvegicus).The method of experimental used Completely Random Design (CRD in triple repetition. The treatment had been carried out orally. Iron dextran with total dose of 1.5 g kg-1 of body were given to rat on the first, fourth, seventh, ten and thirteenth day. Sappan Wood Extract (Caesalpinia sappan)200 mg kg-1 bw, 400 mg kg-1 bw, Wheat grass extract 100 mg kg-1 bw and Vitamin E 60 mg kg-1 bw were given to rat everyday for 15 days. At the seventeenth, rat were killed and their liver were taken. The observed parameters are morphological abnormality including the colour, the contour, ratio between liver weight and body weight as well as histological destruction. The result showed iron dextran treatment was proved the abnormality on morphological and histological desruction. Futhermore, Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment can decrease the morphological abnormality and the liver histological destructionon of iron overload rat. Keywords: Sappan Wood Extract, Wheat grass, Vitamin E, Iron, Morphological abnormality, Histological destruction
The Effectiveness of Antibiotics and Hematopoietic Stem Cell Treatment in Periodontitis Rat Model Toward TNF α Expression
Authors: R Retno Indrawati , Diyatri Indeswati , Rahmawati Dwi 
Published: March 2018

Periodontitis is a biofilm-induced chronic inflammatory. The current therapy of periodontitis is scaling root planning and curettage, and followed by administration of antibiotic such as combination of Amoxicillin and Clavulanic acid. Hematopoietic Stem Cell has ability to differentiate into blood cells which is capable of homing and regenerating itself. Research Purpose: The aim of this study was to prove antibiotic and Hematopoietic Stem Cell administration can reduce TNF α expression. Method: This research was divided into four different groups, P1 as negative control group, P2 as positive control group which inoculated with P.gingivalis 109 for three weeks as chronic periodontitis rat model and USP were administered, P3 as chronic periodontitis rat model received Hematopoietic Stem Cell injection into the tail vein of rat, P4 as chronic periodontitis rat models were given Amoxicillin and Clavulanic acid 250 mg/kg BW orally then followed by Hematopoietic Stem Cell 106 injection into the tail vein of rat. After two weeks rat were sacrified and immunohistochemically analysed for expression of TNF α. The data were analysed by using Non-Parametric Test. Result: TNF α expression of negative control group was different as compared to positive control group and treatment group which given Hematopoietic Stem Cell injection, but there is no difference between negative control group with treatment group which given Amoxicillin and Clavulanic acid 250 mg/kg BW orally and followed by Hematopoietic Stem Cell injection. Clonclusion: The administration of Amoxicillin and Clavulanic acid 250 mg/kg BW and Hematopoietic Stem Cell can reduce TNF α expression on periodontitis rat model.Keyword: Hematopoetic Stem Cell, TNF α, Chronic Periodontitis, Antibiotic
Antiviral Activity Effect of Silver Nanoparticles (Agnps) Solution Against the Growth of Infectious Bursal Disease Virus on Embryonated Chicken Eggs with Elisa Test
Authors: Pangestika Rosa , Ernawati Rahaju 
Published: March 2018

Infectious bursal disease virus is one of the strategic infectious disease in Indonesia. Despite disinfection and vaccination technology has been doing, the cases still frequently occur and it needs another alternative technology to be developed to against IBD virus. This research try to answer the problem, it examines the effect of antiviral activity of silver nanoparticles (AgNPs) solution against the growth of infectious bursal disease virus in embryonated chicken eggs with ELISA Test. The research has two methods, the first method is conducted by mixing a solution of AgNPs and IBD Virus, two hours before inoculated (preventive method) and the second method is the virus inoculated first, 48 hours later the AgNPs solution injected (therapy method). Each method has several dosage of AgNPs solution respectively 0 ppm (positive control 20 ppm, 40 ppm, and 50 ppm). Virus samples taken from the choriallantoic membrane (CAM) and the embryo by crushed method. Results based on the value of OD (optical density) ELISA Test and Statistical Test ANOVA General Linier Models Univariate with Post-Hoc Duncan 5%, both methods have no significant difference (p>0.05), it means the solution of AgNPs has good preventative and therapeutic characteristic. The mean of OD values also showed dosage of 20ppm is most effective dosage in against the growth of the virus, the dosage has significant difference (p<0.05). The decreasing amount of virus in CAM and in embryos were not significantly different (p>0.05), in both CAM and embryo AgNPs solution has good antiviral properties. Keywords : Silver Nanoparticles, Antiviral, Infectious Bursal Disesase, ELISA, Embryonated Chicken Eggs