bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prophylactic Wound Drainage in Renal Transplantation: A Systematic Review
Authors: D’Souza Kenneth , Crowley Sean Patrick , Hameed Ahmer , Lam Susanna , Pleass Henry Claud , Pulitano Carlo , Laurence Jerome Martin 
Published: June 2019

Background.
Adult kidney transplantation is most commonly into an extraperitoneal potential space, and surgically placed drains are used routinely in many centers. There is limited evidence of clinical benefit for prophylactic drainage in other major abdominal and vascular surgery. Transplantation is, however, a unique setting combining organ dysfunction and immunosuppression, and the risks and benefits of prophylactic drain placement are not known. This study attempts to examine existing literature to determine whether prophylactic intraoperative drains have an impact on the likelihood of perigraft fluid collections and other wound-related complications following kidney transplantation.

Methods.
A literature search of MEDLINE and EMBASE was conducted to identify published comparative studies, including recipients receiving prophylactic drains to recipients in whom drains were omitted. The main outcomes were the incidence of peritransplant fluid collections and wound-related complications. Meta-analysis was performed on these data.

Results.
Four retrospective cohort studies were deemed eligible for quantitative analysis and 1 additional conference abstract was included in qualitative discussion. A total of 1640 patients, 1023 with drains and 617 without, were included in the meta-analysis. There was a lower rate of peritransplant collections associated with the drain group (RR 0.62; 95% confidence interval, 0.42-0.90). There was no significant difference in the incidence of wound-related complications between the groups (RR 0.85; 95% confidence interval, 0.34-2.11).

Conclusions.
These data associate a higher rate of peritransplant fluid collections with omission of prophylactic drainage, without a difference in the incidence of wound-related complications. Further research is required to definitively determine the impact of drains in this patient group.
Significant Reduction of Murine Renal Ischemia-Reperfusion Cell Death Using the Immediate-Acting PrC-210 Reactive Oxygen Species Scavenger
Authors: Bath Natalie M. , Fahl William E. , Redfield Robert R. 
Published: June 2019

Background.
Ischemia-reperfusion (IR) injury remains a significant problem for all solid organ transplants; thus, an important unmet need in transplantation is the prevention of IR injury. PrC-210 has demonstrated superior prevention of reactive oxygen species damage in several preclinical studies as a free radical scavenger. Here, we describe its profound efficacy in suppressing IR injury in a murine model of kidney IR injury.

Methods.
C57/B6 mice underwent laparotomy with the left renal pedicle occluded for 30 minutes to induce IR injury. Right nephrectomy was performed at the time of surgery. Mice received a single systemic dose of the PrC-210, PrC-211, or PrC-252 aminothiols 20 minutes before IR injury. Twenty-four hours following IR injury, blood and kidney tissue were collected for analysis. Kidney caspase-3 level (a marker of cell death), direct histological analysis of kidneys, and serum blood urea nitrogen (BUN) were measured in animals to assess reactive oxygen species scavenger protective efficacies.

Results.
A single systemic PrC-210 dose 20 minutes before IR injury resulted in significant reductions in (1) IR-induced kidney caspase level (P < 0.0001); caspase was reduced to levels not significantly different than control caspase levels seen in unperturbed kidneys, (2) IR-induced renal tubular injury scores (P < 0.0001); brush border loss and tubular dilation were markedly reduced, and (3) serum BUN compared with control IR injury kidneys (P < 0.0001). The ranked protective efficacies of PrC-210 > PrC-211 >> PrC-252 paralleled previous radioprotection studies of the molecules.

Conclusions.
A single PrC-210 dose, minutes before the IR insult, profoundly reduced caspase, renal tubular injury, and serum BUN in mice exposed to standard kidney IR injury. These findings support further development of the PrC-210 molecule to suppress or prevent IR injury in organ transplant and other IR injury settings.
Effect of Sodium Bicarbonate in Kidney Transplant Recipients With Chronic Metabolic Acidosis
Authors: Schulte Kevin , Püchel Jodok , Schüssel Katrin , Borzikowsky Christoph , Kunzendorf Ulrich , Feldkamp Thorsten 
Published: June 2019

Background.
Metabolic acidosis (MA) is a common complication after kidney transplantation and regarded to increase mortality, graft failure, and bone fractures. Here, we conducted a retrospective cohort study to analyze the effect of sodium bicarbonate on those events.

Methods.
All kidney transplant recipients of the German health insurance Allgemeine Ortskrankenkasse (AOK) were selected, who received their transplantation between 2007 and 2015. Three groups were formed: (1) control group (no acidosis, n = 3602), (2) acidosis group (encoded acidosis, n = 370), and (3) treatment group (encoded therapy, n = 769). The study endpoints were mortality, death-censored graft failure, and bone fractures.

Results.
The prevalence of MA in the first year after transplantation was 46.2%. The 5-year patient and graft survival were 89.8% and 89.3% in the control group, 90% and 90.8% in the acidosis group, and 87.5% and 81.6% in the treatment group, respectively. The rate of bone fractures did not differ between the groups. Neither log-rank tests nor multivariable Cox regression analyses could detect a negative impact of MA on mortality (hazard ratio [HR] 0.94; confidence interval [CI] 0.67–1.30), graft failure (HR1.18; CI 0.82–1.72), or the incidence of bone fractures (HR1.19; CI 0.92–1.55). Treatment with sodium bicarbonate was associated with an increased risk of graft failure (HR1.52; CI 1.03–2.25), whereas mortality (HR0.86; CI 0.59–1.26) and the incidence of bone fractures (HR1.16; CI 0.86–1.56) were not altered.

Conclusions.
MA is common after kidney transplantation but not associated with an increased frequency of death, graft failure, or bone fractures. Conversely, sodium bicarbonate therapy increased the incidence of graft failure.
Cost-Sensitive Machine Learning Classification for Mass Tuberculosis Screening
Authors: Septiandri Ali Akbar , Aditiawarman  , Tjiong Roy , Burhan Erlina , Shankar Anuraj H. 
Published: June 2019

AbstractActive screening for Tuberculosis (TB) is needed to optimize detection and treatment. However, current algorithms for verbal screening perform poorly, causing misclassification that leads to missed cases and unnecessary and costly laboratory tests for false positives. We investigated the role of machine learning to improve the predefined one-size-fits-all algorithm used for scoring the verbal screening questionnaire. We present a cost-sensitive machine learning classification for mass tuberculosis screening. We compared score-based classification defined by clinicians to machine learning classification such as SVM-RBF, logistic regression, and XGBoost. We restricted our analyses to data from adults, the population most affected by TB, and investigated the difference between untuned and unweighted classifiers to the cost-sensitive ones. Predictions were compared with the corresponding GeneXpert MTB/Rif results. After adjusting the weight of the positive class to 40 for XGBoost, we achieved 96.64% sensitivity and 35.06% specificity. As such, sensitivity of our identifier increased by 1.26% while specificity increased by 13.19% in absolute value compared to the traditional score-based method defined by our clinicians. Our approach further demonstrated that only 2000 data points were sufficient to enable the model to converge. Our results indicate that even with limited data we can actually devise a better method to identify TB suspects from verbal screening. This approach may be a stepping stone towards more effective TB case identification, especially in primary health centres, and foster better detection and control of TB.
Association of puberty timing with Type 2 diabetes: Systematic review and meta-analysis
Authors: Cheng Tuck Seng , Day Felix R. , Lakshman Rajalakshmi , Ong Ken K. 
Published: June 2019

OBJECTIVEWe aimed to systematically review published evidence on the association between puberty timing and Type 2 diabetes or impaired glucose tolerance (T2D/IGT), with and without adjustment for adiposity, and to estimate its potential contribution to the burden of T2D.RESEARCH DESIGN AND METHODSWe searched PubMed, Medline and Embase databases for publications until February 2019 on the timing of any secondary sexual characteristic in boys or girls in relation to T2D/IGT. Inverse-weighted random-effects meta-analysis was used to pool reported estimates and meta-regression to explore sources of heterogeneity.RESULTSTwenty eight observational studies were identified. All assessed age at menarche (AAM) in women (combined N=1,228,306); only one study additionally included men. In models without adjustment for adult adiposity, T2D/IGT risk was higher per year earlier AAM (relative risk (RR)=0.91, 95% confidence interval (CI)=0.89-0.93, 11 estimates, n=833,529, I2=85.4%) and for early versus later menarche (RR=1.41, 95% CI=1.28-1.55, 23 estimates, n=1,185,444, I2=87.8%). Associations were weaker but still evident in models adjusted for adiposity (AAM: RR=0.97 per year, 95% CI=0.95-0.98, 12 estimates, n=852,268, I2=51.8%; early menarche: RR=1.19, 95% CI=1.11-1.28, 21 estimates, n=890,583, I2=68.1%). Associations were stronger among Caucasians than Asians, and in populations with earlier average AAM. The estimated population attributable risk of T2D in UK Caucasians due to early menarche, unadjusted and adjusted for adiposity, was 12.6% (95% CI=11.0-14.3) and 5.1% (95% CI=3.6-6.7), respectively.CONCLUSIONSA substantial proportion of T2D in women is attributable to early menarche timing. This will increase in light of global secular trends towards earlier puberty timing.
Maternal Mortality and Women’s Political Participation
Authors: Bhalotra Sonia , Clarke Damian , Gomes Joseph , Venkataramani Atheendar 
Published: June 2019

AbstractWe show that large declines in maternal mortality can be achieved by raising women’s political participation. We estimate that the recent wave of quotas for women in parliament in low income countries has resulted in a 9 to 12% decline in maternal mortality. Among mechanisms are that gender quotas lead to an 8 to 10% increase in skilled birth attendance, a 6 to 12% increase in prenatal care utilization and a 4 to 11% decrease in birth rates.JEL codesI14, I15, O15.
Epigenetic prediction of major depressive disorder
Authors: Barbu Miruna C. , Walker Rosie M. , Howard David M. , Evans Kathryn L. , Whalley Heather C. , Porteous David J. , Morris Stewart W. , Deary Ian J. , Marioni Riccardo E. , Clarke Toni-Kim , McIntosh Andrew M. 
Published: June 2019

AbstractObjectiveDNA methylation (DNAm) is associated with environmental risk factors for major depressive disorder (MDD) but has not yet been tested for its ability to discriminate individuals with MDD from unaffected individuals.MethodsUsing penalized regression based on genome-wide CpG methylation, we trained a DNAm risk score of MDD (DNAm-RS) in 1,223 cases and 1,824 controls and tested in a second independent sample of 363 prevalent cases and 1,417 controls. Using DNA from 1,607 unaffected individuals, we tested whether DNAm-RS could discriminate the 190 incident cases of lifetime MDD from the 1,417 individuals who remained unaffected at follow-up.ResultsA weighted linear combination of 196 CpG sites were derived from the training sample to form a DNAm-RS. The DNAm-RS explained 1.75% of the variance in MDD risk in an independent case-control sample and significantly predicted future incident episodes of MDD at follow up (R2=0.52%). DNAm-RS and MDD polygenic risk scores together additively explained 3.99% of the variance in prevalent MDD. The DNAm-RS was also significantly associated with lifestyle factors associated with MDD, including smoking status (β=0.440, p=<2×10−16) and alcohol use (β=0.092, p=9.85×10−5). The DNAm-RS remained significantly associated with MDD after adjustment for these environmental factors (independent association: β=0.338, p=1.17×10−7 association post-adjustment: β=0.081, p=0.0006).ConclusionsA novel risk score of MDD based on DNAm data significantly discriminated MDD cases from controls in an independent dataset, and controls who would subsequently develop MDD from those who remained unaffected. DNAm-RS captured the effects of exposure to key lifestyle risk factors for MDD, revealing a potential role in risk stratification.
Temporal and spatial limitations in global surveillance for bat filoviruses and henipaviruses
Authors: Becker Daniel J. , Crowley Daniel E. , Washburne Alex D. , Plowright Raina K. 
Published: June 2019

AbstractSampling reservoir hosts over time and space is critical to detect epizootics, predict spillover, and design interventions. However, because sampling is logistically difficult and expensive, researchers rarely perform spatiotemporal sampling of many reservoir hosts. Bats are reservoirs of many virulent zoonotic pathogens such as filoviruses and henipaviruses, yet the highly mobile nature of these animals has limited optimal sampling of bat populations. To quantify the frequency of temporal sampling and to characterize the geographic scope of bat virus research, we here collated data on filovirus and henipavirus prevalence and seroprevalence in wild bats. We used a phylogenetically controlled meta-analysis to next assess temporal and spatial variation in bat virus detection estimates. Our analysis shows that only one in four bat virus studies report data longitudinally, that sampling efforts cluster geographically (e.g., filovirus data are available across much of Africa and Asia but are absent from Latin America and Oceania), and that sampling designs and reporting practices may affect some viral detection estimates (e.g., filovirus seroprevalence). Within the limited number of longitudinal bat virus studies, we observed high heterogeneity in viral detection estimates that in turn reflected both spatial and temporal variation. This suggests that spatiotemporal sampling designs are essential to understand how zoonotic viruses are maintained and spread within and across wild bat populations, which in turn could help predict and preempt risks of zoonotic viral spillover.
Physical activity and screen time in children who survived cancer – A report from the Swiss Childhood Cancer Survivor Study
Authors: Schindera Christina , Weiss Annette , Hagenbuch Niels , Otth Maria , Diesch Tamara , von der Weid Nicolas , Kuehni Claudia E. ,   for the Swiss Pediatric Oncology Group (SPOG) Scientific Committee
Published: June 2019

ABSTRACTBackgroundPhysical activity (PA) can reduce the risk of chronic adverse health conditions in childhood cancer survivors. We examined physical activity and sedentary screen time behavior in a nationwide study in Switzerland.ProceduresThe Swiss Childhood Cancer Survivor Study sent questionnaires to parents of all Swiss resident ≥5 year-survivors diagnosed 1995–2010. We assessed physical activity including compulsory school sport, recreational sport, commuting to school, and time spent with screen media in those aged 5–15 years, and compared results to international recommendations.ResultsWe included 766 survivors with a median age at diagnosis of 2.8 (interquartile range 1.4–5.0) years and a median age at study of 12.5 (10.0–14.3) years. Median PA time was 7.3 (4.8–10.0) hours/week and median screen time 1.4 (0.8–2.0) hours/day. Compulsory school sport hours and walking or cycling to school contributed significantly to total PA. 55% of survivors met PA and 68% screen time recommendations. PA was lower for children living in regions of Switzerland speaking French or Italian compared to German, and for those who had a relapse or musculoskeletal/neurological conditions. Screen time was higher in males, and children with lower parental education and a migration background.ConclusionsPA and sedentary screen watching were associated with social factors and PA also with clinical risk factors. Structural preventions that afford active commuting to school and sufficient school sport are essential, as is counselling vulnerable survivor groups such as those with musculoskeletal and neurological problems, and those who have had a relapse.
Retrograde mitochondrial transport is essential for mitochondrial homeostasis in neurons
Authors: Mandal Amrita , Pinter Katherine , Mosqueda Natalie , Beirl Alisha , Lomash Richa Madan , Won Sehoon , Kindt Katie S , Drerup Catherine M 
Published: June 2019

AbstractMitochondrial transport in neurons is essential for forming and maintaining axonal projections. While much is known about anterograde mitochondrial movement, the function of retrograde mitochondrial motility in neurons was unknown. We investigated the dynamics and utility of retrograde mitochondrial transport. Using long-term tracking of mitochondria in vivo, we found mitochondria in axon terminals turnover within hours via retrograde transport. Mitochondria do not return to the cell body solely for degradation; rather, mitochondria use bidirectional transport to redistribute themselves throughout the neuron. Disruption of retrograde mitochondrial transport severely depletes the cell body of mitochondria and impacts mitochondrial health throughout the cell. Altered mitochondrial health correlates with decreased synaptic activity. Using proteomics, we provide evidence that retrograde mitochondrial movement functions to maintain the organelle’s proteome. Together, our work demonstrates that mitochondrial retrograde transport is essential for the maintenance of a homeostatic population of mitochondria in neurons and consequently effective synaptic activity through promoting mitochondrial protein turnover.
Neuromodulatory selection of motor neuron recruitment patterns in a visuomotor behavior increases speed
Authors: Jha Urvashi , Thirumalai Vatsala 
Published: June 2019

SummaryAnimals generate locomotion at different speeds to suit their behavioral needs. Spinal circuits generate locomotion at these varying speeds by sequential activation of different spinal interneurons and motor neurons. Larval zebrafish can generate slow swims for prey capture and exploration by activation of secondary motor neurons and much faster and vigorous swims during escapes and struggles via the additional activation of primary motor neurons. Neuromodulators are known to alter motor output of spinal circuits yet their precise role in speed regulation is not understood well. Here, in the context of optomotor response (OMR), an innate, evoked locomotor behavior, we show that dopamine (DA) provides an additional layer to regulation of swim speed in larval zebrafish. Activation of D1-like receptors increases swim speed during OMR in free-swimming larvae. By analysing tail bend kinematics in head-restrained larvae, we show that the increase in speed is actuated by larger tail bends. Whole cell patch clamp recordings from motor neurons reveal that during OMR, typically only secondary motor neurons are active while primary motor neurons are quiescent. Activation of D1-like receptors increases motor drive from secondary motor neurons by decreasing spike threshold and latency. In addition, D1-like receptor activation enhances excitability and recruits quiescent primary motor neurons. Our findings provide an example of neuromodulatory reconfiguration of spinal motor neuron speed modules such that members are selectively recruited and motor drive is increased to effect changes in locomotor speed.HighlightsZebrafish larvae generate swims of increased speed during optomotor response when D1-like receptors are activated.D1-like receptor activation increases the extent of tail bending during forward swims and turns resulting in increased swim speed.Neuromodulation via D1-like receptors increases motor drive by enhancing excitability of ‘slow’ motor neurons. In addition, D1-like receptor activation recruits quiescent ‘fast’ motor neurons to increase swim speed.This demonstrates neuromodulatory selection of motor neurons belonging to different ‘speed’ modules to alter swimming behavior.Graphical abstract
How does grasshopperOxya chinensisrespond to the artificially infected bacteria?
Authors: Zhang Xiaomin , Zhang Keshi 
Published: June 2019

AbstractOxya chinensisis one of the most widespread grasshopper species found in China and one of the most common pests against rice. Due to the importance of haemocytes in insect immunity and limited information on the haemocytes ofO. chinensis, their haemocytes were examined in detail. The cellular response of the grasshopper was challenged with bacteriaEscherichia coli,Staphylococcus aureusandBacillus subtilis. The morphology of the haemocytes was illustrated by the use of light, scanning electron and transmission electron microscopy, where different morphological varieties of haemocytes were observed. Granulocytes and plasmatocytes responded to the challenged bacteria by phagocytosis. The histochemical staining has indicated the presence of acid phosphatase in plasmatocytes and granulocytes. Non-phagocytic prohemocytes and vermicytes were noticed, but their functions in the circulation are unclear. Our results demonstrate an essential role of plasmatocytes and granulocytes in the innate immunity ofO. chinensis. Insect haemocytes play a crucial role in cellular immunity, and further research is needed for a comprehensive understanding.Summary statementThe cellular response was used byOxya chinensisagainst the bacterial challenges where two types of haemocytes shared the duty of phagocytosis.
Di-Ras2 Promotes Renal Cell Carcinoma Formation by Activating the MAPK Pathway in the Absence of VHL
Authors: Rao Hanyu , Li Xuefeng , Liu Min , Liu Jing , Li Xiaoxue , Xu Jin , Li Li , Gao Wei-Qiang 
Published: June 2019

AbstractClear cell renal cell carcinoma (ccRCC) is one of the most common and lethal human urological malignancies in the world. The pathological drivers for ccRCC are still poorly understood. One of them is the Ras family of small GTPases that function as “molecular switches” in many diseases including ccRCC. Among them, Di-Ras2 encodes a 26-kDa GTPase that shares 60% homology to Ras and Rap. Yet little is known about the biological function(s) of Di-Ras2. In this study, we found that Di-Ras2 was upregulated in ccRCC, and promoted the proliferation, migration and invasion of human ccRCC cells in the absence of von Hippel-Lindau (VHL). Mechanistically, Di-Ras2 induces and regulates ccRCC formation by modulating phosphorylation of the downstream effectors and activating the MAPK signaling pathway. Moreover, Di-Ras2 interacts with E3 ubiquitin ligase, VHL, which facilitates the ubiquitination and degradation of Di-Ras2. Together, these results indicate a potential function of Di-Ras2 as an oncogene and biomarker in ccRCC, and these data provide a new perspective of the relationship between VHL and the MAPK pathway in ccRCC tumorigenesis.