bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum microRNA qPCR profiling and validation indicate upregulation of circulating miR-145-5p and miR-26a-5p in migraineurs
Authors: Kordacka Joanna , Gruszka Renata , Zakrzewska Magdalena 
Published: November 2024

Abstract
Background
In recent years, miRNAs found in biological fluids have gained interest as biomarkers of numerous conditions, including migraine. This study aimed to identify differences in the levels of circulating miRNAs in the serum of migraineurs as compared to healthy controls, as well as between patients with different types of migraine and during the ictal and nonictal phases of the condition.

Methods
The screening phase of the study included serum from 13 migraine patients and 13 sex and age matched controls. A panel of 179 miRNAs was analysed using locked nucleic acid SYBR based qPCR. Based on statistical analysis (U Mann-Whitney test) and data from existing literature, nine miRNAs were selected for validation by TaqMan qPCR in an independent cohort of 26 migraineurs and eleven healthy controls. For comparison between the study and control group, U Mann-Whitney test was performed. The differences between patients with chronic and episodic migraine, migraine with and without aura and in ictal and nonictal phases were analysed with Kruskal-Wallis test. The results were corrected for multiple comparisons using Benjamini-Hochberg method. In all analysis p value ≤ 0,05 was considered as significant.

Results
Two miRNAs, miR-145-5p and miR-26a-5p were significantly upregulated in serum of migraineurs compared to healthy controls. MiRNA-19a-3p was downregulated in patients currently experiencing migraine headache compared to those in the interictal period. No differences were found between patients with different migraine types.

Conclusion
The results of our study add to the growing body of evidence for dysregulation of the circulating miRNA profile by migraine. They are further supported by previous reports on differential expression of miR-145-5p, miR-26a-5p and miR-19a-3p in migraineurs. However, more research on larger populations is needed to validate these findings, as well as elucidate the role of circulating miRNAs in the condition. Moreover, to wholly explore the biomarker potential of miRNAs, migraine patients should not only be compared to healthy controls but also to populations with different headache disorders.

Graphical Abstract
Dose-related effect of acetylcholine on human gingival blood flow
Authors: Nagy Tamás László , Mikecs Barbara , Lohinai Zsolt M. , Vág János 
Published: November 2024

Abstract
Background
This study investigates the dose-response relationship of acetylcholine (ACh) on healthy human gingival blood flow (GBF). Understanding this dose-response relationship contributes to studying vasodilatory mechanisms in various pathological conditions.

Methods
The study involved 22 young healthy men (21 - 32 years) to investigate the dose-response relationship of ACh on GBF. Semi-circular wells were created on the labial surface of the upper right second incisor (FDI #12) and upper left first incisor (FDI #21), including the gingival sulcus, for the application of drugs. ACh-chloride solutions at 0.1, 1, and 10 mg/mL were administered to the gingival sulcus of tooth FDI #12 with a Hamilton syringe. Physiological saline was applied on the contralateral side to FDI #21 as a control. The GBF was measured non-invasively by the laser speckle contrast imaging method in four 1mm high adjacent regions: coronal, midway1, midway2, and apical, and was expressed in a laser speckle perfusion unit (LSPU). After the baseline blood flow recording, ACh doses were applied sequentially, with washout periods in between. Data were statistically analyzed using a linear mixed model.

Results
The GBF did not change on the saline site throughout the experiment. The GBF was significantly higher at the coronal region after all ACh doses (baseline: 218±31 LSPU, and 227±38 LSPU p < 0.05, 239±40 LSPU p < 0.001, 291±54 LSPU p < 0.001, respectively) compared to the saline. It was also elevated following 1 and 10 mg/mL at the midway1 (245±48 LSPU,
p < 0.05, 293±65 LSPU p < 0.001). At midway2 and apical, only the 10 mg/mL dose was effective (285±71 LSPU, p < 0.001; 302±82 LSPU, p < 0.001).

Conclusions
Our findings suggest a dose-dependent vasodilation to ACh, emphasizing its role in human gingival microcirculation. Only the 10 mg/mL ACh could evoke remote vasodilation 3 mm from the application. The described method could facilitate the investigation of endothelium-dependent vasodilation in disorders affecting microcirculation, such as periodontitis or diabetes.
Healthcare providers’ hospital breastfeeding practices during the COVID-19 endemic and associated factors in Thailand: a cross-sectional study
Authors: Lawin Nongyao , Nuampa Sasitara , Somsuk Chananchida , Srisawad Sutthisak , Raungrongmorakot Kasem , Ketsuwan Sukwadee 
Published: November 2024

Abstract
Background
During COVID-19, healthcare providers were limited in their ability to provide breastfeeding support while women encountered breastfeeding difficulties. Enhancing appropriate breastfeeding care practices among healthcare providers in hospitals may improve the safety of breastfeeding during an endemic. However, little is known about the breastfeeding care practices by healthcare providers and associated factors during the endemic impact.

Objective
To investigate the effect of the endemic on breastfeeding care practices by healthcare providers in hospitals and examine their associated factors in Thailand.

Methods
A descriptive comparative design was conducted through an online survey with 350 healthcare providers across five regions of Thailand between January and March 2022. The convenience sampling was used to recruit healthcare providers who had at least two years of experience supporting breastfeeding practices and were full-time working in the obstetric and pediatric departments of public tertiary hospitals. Analysis of variance and the independent t-test with relevant statistical corrections were utilized for comparisons of associated factors on breastfeeding care practices in healthcare providers.

Results
The mean breastfeeding care practices in hospitals during the COVID-19 endemic by healthcare providers was 39.17 (SD = 4.64, range 23 to 50). Four factors were statistically significant differences in breastfeeding care practices score, including work position (F = 7.03, df = 2.0, p = 0.001), types of COVID-19 vaccination (F = 6.95, df = 2, p = 0.001), education (F = 4.78, df = 2, p = 0.009), and monthly family income (F = 4.25, df = 3, p = 0.006), respectively. In addition, dose of COVID-19 vaccination and types of COVID-19 vaccination were significantly associated with individual breastfeeding support in hospitals (p < 0.05).

Conclusions
Healthcare providers’ breastfeeding care practices in hospitals during the COVID-19 endemic were mostly at a moderate level in the Thai context. Hospital policy for maternal and child health support should strongly recommend the effective and safe practice of breastfeeding to encourage mothers to continue their breastfeeding duration.
Systematic review of Mendelian randomization studies on antihypertensive drugs
Authors: Fan Bohan , Zhang Junmeng , Zhao Jie V. 
Published: November 2024

Abstract

Background
We systematically reviewed Mendelian randomization (MR) studies and summarized evidence on the potential effects of different antihypertensive drugs on health.

Methods
We searched PubMed and Embase for MR studies evaluating the effects of antihypertensive drug classes on health outcomes until 22 May 2024. We extracted data on study characteristics and findings, assessed study quality, and compared the evidence with that from randomized controlled trials (RCTs).

Results
We identified 2643 studies in the search, of which 37 studies were included. These studies explored a wide range of health outcomes including cardiovascular diseases and their risk factors, psychiatric and neurodegenerative diseases, cancer, immune function and infection, and other outcomes. There is strong evidence supporting the protective effects of genetically proxied antihypertensive drugs on cardiovascular diseases. We found strong protective effects of angiotensin-converting enzyme (ACE) inhibitors on diabetes whereas beta-blockers showed adverse effects. ACE inhibitors might increase the risk of psoriasis, schizophrenia, and Alzheimer’s disease but did not affect COVID-19. There is strong evidence that ACE inhibitors and calcium channel blockers (CCBs) are beneficial for kidney and immune function, and CCBs showed a safe profile for disorders of pregnancy. Most studies have high quality. RCT evidence supports the beneficial effects of ACE inhibitors and CCBs on stroke, diabetes, and kidney function. However, there is a lack of reliable RCTs to confirm the associations with other diseases.

Conclusions
Evidence of the benefits and off-target effects of antihypertensive drugs contribute to clinical decision-making, pharmacovigilance, and the identification of drug repurposing opportunities.
Traditional complementary and alternative medicine (TCAM) use among PLHIV on antiretroviral medication
Authors: Denu Mawulorm KI , Buadu Maame Araba E. , Adrah Frederick , Normeshie Cornelius A. , Berko Kofi Poku 
Published: November 2024

Abstract
Background
Traditional complementary and alternative medicine (TCAM) are products and practices that differ from conventional allopathic medicine. There continues to be an increase in the use of these methods of treatment in developed and developing countries worldwide. This often owes to the perceived ability of these treatment methods to cure chronic medical conditions like HIV. However, TCAM use among PLHIV may be associated with reduced compliance with antiretroviral medications, resulting in poor viral load suppression and increased risk for opportunistic infections. The concomitant use of antiretroviral drugs and TCAM practices may be influenced by some sociodemographic and health-related factors.

Objective
To determine the prevalence of TCAM use and examine the sociodemographic and health-related factors associated with its use among PLHIV on antiretroviral medications at the Infectious Disease unit of Korle-Bu Teaching Hospital in Ghana.

Methods
A cross-sectional study was conducted among attendants at an adult HIV clinic. 420 study participants were selected by systematic sampling. Data related to TCAM use, sociodemographic and health-related factors were collected using a standardized questionnaire and patient chart review. Multivariate logistic regression model was used to determine the association between TCAM use, sociodemographic and health-related factors.

Results
Of the 420 study participants, majority were female (76.2%) and urban community dwellers (77.9%). 77.4% of participants had been diagnosed with HIV for $$\:\ge\:$$ 5 years and had been on anti-retroviral medications for more than 5 years. The prevalence of TCAM use among PLHIV was 25.2%. No sociodemographic or HIV-related health factor was significantly associated with TCAM use in the study population.

Conclusion
TCAM use was high among PLHIV. No sociodemographic or health-related factor was found to be associated with TCAM use. Further studies employing a qualitative approach using key informant interviews and focused group discussions are needed to explore reasons for its use. Care providers and policy-makers should look beyond sociodemographic and health-related factors in addressing TCAM use among PLHIV.
Subcutaneous delivery of mesenchymal stromal cells induces immunoregulatory effects in the lymph node prior to their apoptosis
Authors: Zheng Di , Bhuvan Tejasvini , Payne Natalie L. , Pang Swee H. M. , Mendonca Senora , Hutchinson Mark R. , McKinnirey Flyn , Morgan Charlotte , Vesey Graham , Meagher Laurence , Heng Tracy S. P. 
Published: November 2024

Abstract
Background
Mesenchymal stromal cell (MSC) therapy commonly involves systemic infusion of MSCs, which undergo apoptosis in the lung and induce immunoregulatory macrophages that reduce disease. The relevance of this mode of action, however, is yet to be determined for MSCs administered via other routes. Here, we administered MSCs via subcutaneous (SC) injection into inflamed tissue and investigated the immunomodulatory effects on the local lymph node (LN), which is a major site for the initiation and regulation of immune responses.

Methods
A mouse model of localised skin inflammation was established with low-dose lipopolysaccharide (LPS) to in vivo prime adipose-derived MSCs delivered via SC injection. We then analysed the immunomodulatory changes in the LN draining the inflamed tissue, as well as the neutrophil TNF response to LPS re-exposure.

Results
When administered directly into the inflamed skin tissue, SC MSC injection induced an expansion of IL-10-producing MerTK+ subcapsular sinus macrophages and T cell zone macrophages, as well as activated CD44+ regulatory T cells (Tregs), in the draining LN, which was not observed in the non-draining LN. SC injection of viable, but not apoptotic, MSCs dampened TNF production by inflammatory cells in the draining LN when re-exposed to the inflammatory stimulus. SC injection of MSCs remote to the site of inflammation also did not attenuate the LN response to subsequent inflammatory challenge.

Conclusions
MSCs delivered directly into inflamed skin activated immunoregulatory cells in the local LN and inhibited LN responsiveness to subsequent inflammatory challenge. The immunoregulatory effects of SC-injected MSCs in the LN require priming by inflammatory cytokines in the local milieu. Furthermore, SC-injected MSCs exert anti-inflammatory effects in the draining LN prior to their apoptosis, in contrast to intravenously delivered MSCs, where anti-inflammatory effects are linked to their apoptosis.
A multicentric survey and single-centre observational study of usage behaviour of sinks in intensive care: training is needed to minimize risk
Authors: Fucini Giovanni-Battista , Abe Robert , Lemke Elke , Gastmeier Petra 
Published: November 2024

Abstract
Introduction
Sinks have been introduced near patients to improve hand hygiene as part of infection prevention and control measures. However, sinks are a known reservoir for gram-negative bacterial pathogens in particular and their removal to prevent bacterial infections in intensive care patients is currently recommended by several international guidelines.

Methods
Healthcare workers (HCWs) in 15 intensive care units (ICUs) in Germany were given the opportunity to complete an anonymous survey on the use of sinks between August 2022 and January 2023. Observations were then made in three participating ICUs to determine the frequency and reason for contact with the sink.

Results
258 questionnaires were returned (nurses 87%). 90% found it useful to very useful to have a sink in the patient room, and 56% reported using it daily for hand hygiene. We observed 33 contacts between nurses and sinks over 17 h. In 20/33 (60%) cases, the sink was used for waste disposal. In 3/33 (10%) it was used for hand washing.

Discussion
Sinks are still used for daily care in intensive care units. Educational Interventions in existing buildings to minimise risk through “sink hygiene” (i.e. separation of sinks for water disposal and uptake) can make an important contribution to infection prevention.
Threat of deepfakes to the criminal justice system: a systematic review
Authors: Sandoval Maria-Paz , de Almeida Vau Maria , Solaas John , Rodrigues Luano 
Published: November 2024

Abstract
Background
This systematic review explores the impact of deepfakes on the criminal justice system. Deepfakes, a sophisticated form of AI-generated synthetic media, have raised concerns due to their potential to compromise the integrity of evidence and judicial processes. The review aims to assess the extent of this threat, guided by a research question: (1) What threats do deepfakes pose to the criminal justice system?

Methods
The review was conducted using databases such as Web of Science, ProQuest, Scopus, and Google Scholar, focusing on publications from 2021 to 2022. Search terms were optimised for sensitivity and specificity, and articles were chosen based on criteria including relevance to deepfake threats and deepfake detection research. The methodology included rigorous screening processes using tools like Zotero and Rayyan.ai, with an emphasis on inter-rater reliability to ensure objective selection of studies.

Results
The search initially identified 1355 articles, with 1200 articles screened for eligibility after duplicates were removed. For the threat of deepfakes to the criminal justice system, 110 studies were selected for full-text review, and 44 were included in the final analysis. Key findings include identification of primary crime categories linked to deepfakes, such as pornography, fraud, and information manipulation, alongside challenges like trust erosion in institutions and evidence falsification issues.

Conclusions
Deepfakes significantly threaten the criminal justice system, highlighting the necessity for advanced detection methods. These findings underscore the importance of continued research and development in deepfake detection technologies and strategies for legal safeguards and broader implications on policy, national security, and democratic processes.
Technology and innovation in shaping the future of education
Authors: Çelik Ferdi , Baturay Meltem Huri 
Published: November 2024

AbstractThis paper highlights the potential of technology and innovation in advancing education. We discuss how emerging technologies can enhance teaching and learning experiences. We underscore challenges and opportunities in integrating technology into educational practices through an overview of current trends and practices. A balanced approach emphasizing technological integration and pedagogical innovation is essential for preparing students to succeed in an increasingly demanding world. Thus, this paper highlights the importance of fostering a learning environment that prioritizes learner-centered approaches and encourages educators to innovate by utilizing the power of technology to create meaningful educational experiences.
Overexpression of autophagy enhancer PACER/RUBCNL in neurons accelerates disease in the SOD1G93A ALS mouse model
Authors: Labrador Luis , Rodriguez Leonardo , Beltran Sebastián , Hernandez Fernanda , Gomez Laura , Ojeda Patricia , Bergmann Cristian , Calegaro-Nassif Melissa , Kerr Bredford , Medinas Danilo B. , Manque Patricio , Woehlbier Ute 
Published: November 2024

AbstractAmyotrophic lateral sclerosis (ALS) is a debilitating and fatal paralytic disorder associated with motor neuron death. Mutant superoxide dismutase 1 (SOD1) misfolding and aggregation have been linked to familial ALS, with the accumulation of abnormal wild-type SOD1 species being also observed in postmortem tissue of sporadic ALS cases. Both wild-type and mutated SOD1 are reported to contribute to motoneuron cell death. The autophagic pathway has been shown to be dysregulated in ALS. Recent evidence suggests a dual time-dependent role of autophagy in the progression of the disease. PACER, also called RUBCNL (Rubicon-like), is an enhancer of autophagy and has been found diminished in its levels during ALS pathology in mice and humans. Pacer loss of function disturbs the autophagy process and leads to the accumulation of SOD1 aggregates, as well as sensitizes neurons to death. Therefore, here we investigated if constitutive overexpression of PACER in neurons since early development is beneficial in an in vivo model of ALS. We generated a transgenic mouse model overexpressing human PACER in neurons, which then was crossbred with the mutant SOD1G93A ALS mouse model. Unexpectedly, PACER/SOD1G93A double transgenic mice exhibited an earlier disease onset and shorter lifespan than did littermate SOD1G93A mice. The overexpression of PACER in neurons in vivo and in vitro increased the accumulation of SOD1 aggregates, possibly due to impaired autophagy. These results suggest that similar to Pacer loss-of function, Pacer gain-of function is detrimental to autophagy, increases SOD1 aggregation and worsens ALS pathogenesis. In a wider context, our results indicate the requirement to maintain a fine balance of PACER protein levels to sustain proteostasis.
Development, characterization, and assessment of PLAROsomal vesicular system of curcumin for enhanced stability and therapeutic efficacy
Authors: Bhinge Somnath Devidas , Kamble Sheetal , Randive Dheeraj , Bhutkar Mangesh , Nadaf Sameer , Merekar Abhijit , Sonawane Kailas , Jadhav Namdeo , Makandar Asiya , Khan Mohd Shahnawaz , Gurav Shailendra 
Published: November 2024

Abstract
Background
Curcumin (CUR) is a natural polyphenol and one of the key phytoconstituents found in the rhizomes of Curcuma Longa. It exhibits various pharmacological properties, encompassing antioxidant, anticancer effects, antiseptic, and anti-inflammatory, among several others.
A significant drawback of using CUR is its limited bioavailability, which primarily depends on gut microorganisms responsible for converting it into its bioavailable form. Therefore, the contemporary study intended to formulate a novel PLAROsomal vesicular delivery of CUR, i.e., CUR-PLAROsomes employing a design of experiments approach to examine the influence of various process parameters, such as particle size and drug percentage release.

Result
The prepared CUR-PLAROsomes were characterized for their physicochemical properties using various hyphenated tools. The CUR-PLAROsomes exhibited sizes ranging from 40 to 300 nm, and the optimized batch demonstrated a drug entrapment of 86.38 ± 0.22%.
In-vitro anticancer studies were conducted using human colorectal adenocarcinoma cells (COLO320DM) and human breast adenocarcinoma (MCF-7). CUR-PLAROsomes exhibited significant in-vivo anti-inflammatory potential against carrageenan-induced paw edema. CUR-PLAROsomes were more potent against COLO320DM and MCF-7 cell lines, even at lower concentrations, than pure CUR.

Conclusion
Furthermore, based on the observations, it exhibits potential as an anti-inflammatory agent, suggesting that PLAROsomes are an effective vesicular drug delivery system.

Highlights

Newly introduced PLARosome is a next generation of Liposomes which have gain popularity owing to its better adaptability to overcome leakage problem of vesicular drug delivery system.

This is the pioneer attempt to prepare Curcumin-loaded PLARosome as an anti-cancer and anti-inflammatory activity.

Nano size of the PLAROsomes may contribute to enhance the efficacy of Curcumin as a target specific drug delivery system.

Site specific delivery of phytoconstituents is possible by use of PLAROsomes as a novel drug delivery system.

Graphical abstract