bluuebunny/crossref_metadata_2025_split huggingface dataset:
Agomelatine modulates calcium signaling through protein kinase C and phospholipase C‐mediated mechanisms in rat sensory neurons
Authors: Serhatlioglu Ihsan , Bilgin Batuhan , Kacar Emine , Ozcan Sibel , Canpolat Sinan , Ayar Ahmet , Kelestimur Haluk , Ozcan Mete 
Published: November 2018

AbstractAgomelatine, a novel antidepressant exerting its effects through melatonergic and serotonergic systems, implicated to be effective against pain including neuropathic pain but without any knowledge of mechanism of action. To explore the possible role of agomelatine on nociceptive transmission at the peripheral level, the effects of agomelatine on intracellular calcium ([Ca2+]i) signaling in peripheral neurons were investigated in cultured rat dorsal root ganglion (DRG) neurons. Using the fura‐2‐based calcium imaging technique, the effects of agomelatine on [Ca2+]i and roles of the second messenger‐mediated pathways were assessed. Agomelatine caused [Ca2+]i signaling in a dose‐dependent manner when tested at 10 and 100 μM concentration. Luzindole, a selective melatonin receptor antagonist, almost completely blocked the agomelatine‐induced calcium signals. The agomelatine‐induced calcium transients were also nearly abolished following pretreatment with the 100 ng/ml pertussis toxin, a Gi/o protein inhibitor. The stimulatory effects of agomelatine on [Ca2+]i transients were significantly reduced by applications of phospholipase C (PLC) and protein kinase C (PKC) blockers, 10 μM U73122, and 10 μM chelerythrine chloride, respectively. The obtained results of agomelatine‐induced [Ca2+]i signals indicates that peripheral mechanisms are involved in analgesic effects of agomelatine. These mechanisms seems to involve G‐protein‐coupled receptor activation and PLC and PKC mediated mechanisms.
Benzo(a)pyrene inhibits endometrial cell apoptosis in early pregnant mice via the WNT5A pathway
Authors: Yi Ting , Liu Min , Li Xueyan , Liu Xueqing , Ding Yubin , He Junlin , Xu Hanting , Gao Rufei , Mu Xinyi , Geng Yanqing , Wang Yingxiong , Chen Xuemei 
Published: November 2018

AbstractBenzo(a)pyrene (BaP) is an endocrine‐disrupting pollutant present in various aspects of daily life, and studies have demonstrated that BaP exerts reproductive toxicity. We previously showed that BaP damages endometrial morphology and decreases the number of implantation sites in early pregnant mice, but the mechanisms underlying these effects remain unclear. The endometrial function is crucial for implantation, which is associated with endometrial cell apoptosis. In this study, we focused on the effect of BaP on endometrial cell apoptosis and the role of WNT signaling during this process. Pregnant mice were gavaged with corn oil (control group) or 0.2 mg·kg−1·day−1BaP (treatment group) from Days 1 to 6 of pregnancy. BaP impaired endometrial function by decreasing the expression of HOXA10 and BMP2, two markers of receptivity and decidualization. WNT5A and β‐catenin were activated in the BaP group. BaP affected the expression of apoptosis‐related proteins and inhibited the apoptosis of endometrial stromal cells. In vitro, human endometrial stromal cells (HESCs) were treated with different concentrations of BaP (dimethyl sulfoxide (DMSO); 5, 10 µM). WNT5A and β‐catenin were also upregulated in the BaP treatment group. HESC apoptosis was restrained by BaP. Inhibiting WNT5A by SFRP5 partially restored the effect of BaP on apoptosis. In summary, these results suggested that BaP exposure during early pregnancy activates WNT5A/β‐catenin signaling pathway, which inhibits the endometrial cell apoptosis and potentially destroys endometrial function.
Crystallization of amorphous‐Si using nanosecond laser interference method
Authors: Kang Min Jin , Kim Minyeong , Hwang Eui Sun , Noh Jiwhan , Shin Sung Tae , Cheong Byoung‐Ho 
Published: November 2018

AbstractLaser crystallization of a 50‐nm thick amorphous‐Si (a‐Si) thin film on glass substrate was examined by a Nd:YAG (λ = 1064 nm) nanosecond laser and a two‐beam laser interference method. In spite of the low absorption rate of the laser wavelength in the a‐Si, crystallized Si ripple patterns were observed following a single laser pulse irradiation. The atomic force microscope (AFM) measurement revealed that surface ripple arrays are protruded as high as 120 nm at the positions corresponding to the maximum laser intensity and the ripples are composed of narrow double peaks with a separation of 1 μm. Raman image mapping was used to plot the spatial distribution of the crystallized Si phase. It was found that a 1064‐nm‐wavelength nanosecond laser could crystallize an a‐Si thin film into polycrystalline‐Si (poly‐Si) by nonlinear absorption under high laser energy irradiation.
Sex bias: Is it pervasive in otolaryngology clinical research?
Authors: Farzal Zainab , Stephenson Elizabeth D. , Kilpatrick Lauren A. , Senior Brent A. , Zanation Adam M. 
Published: November 2018

Objectives/HypothesisRecent initiatives highlight substantial sex bias in biomedical research. The objective was to determine whether sex bias is present in otolaryngology and whether sex is appropriately analyzed as an independent variable in otolaryngology clinical research.Study DesignLiterature review.MethodsWe systematically reviewed all 2016 articles in three major otolaryngology journals: The Laryngoscope, JAMA Otolaryngology–Head and Neck Surgery, and Otolaryngology–Head and Neck Surgery. Extracted data included study origin, location, subspecialty, number/sex of subjects, ≥50% sex matching (SM≥50), and sex‐based statistical analysis.ResultsSix hundred of 1,209 articles comprising original clinical research were reviewed including 8,997,345,495 subjects (males: 3,898,559,264 [43.3%]; females: 5,095,592,583 [56.6%]; and unknown: 3,193,648 [0.04%]). There were 533/600 (88.8%) studies that included both sexes, eight (1.3%) included females only, five (0.8%) included males only, and 56 (9.3%) did not document participant sex. Only 280 studies (46.7%) analyzed data by sex, and 330 studies (60.7%) had SM≥50. Sex‐based statistical analysis and SM≥50 were similar in domestic and international studies (48.7% vs. 42.8% and 60.9% vs. 62%, respectively). Database studies performed sex‐based statistical analysis more frequently than single and multi‐institutional studies (79.1% vs. 40.4% and 43.4%, P < .00001). Analysis by sex was more frequently performed in head and neck surgery (53.6%) and pediatric otolaryngology (51.3%), whereas SM≥50 was highest in pediatric otolaryngology (86.8%) and otology (82.4%).ConclusionsSex bias exists in the clinical otolaryngology literature, with less than half the studies analyzing sex. Acknowledging the intertwinement of sex with disease pathophysiology and outcomes is important. Eliminating sex bias in research and clinical care should become a major focus for otolaryngologists.Level of EvidenceNA Laryngoscope, 129:858–864, 2019
Cutaneous squamous cell carcinoma metastasis to the parotid region lymph nodes
Authors: Myers Larry L. , Ahn Chul 
Published: November 2018

ObjectiveTo determine the effect on survival of periauricular region cutaneous squamous cell carcinomas (cSCC) metastasizing to parotid region and cervical lymph nodes.MethodsFrom May 1995 to October 2013, consecutive patients with cSCC undergoing parotidectomy/neck dissection ≥ 18 years without distant metastasis were included. Demographic, clinical, and pathologic data were analyzed. Uni‐ and multivariate analyses of disease‐specific survival and overall survival were performed. Statistical significance was set at P < 0.05.ResultsWe evaluated 137 patients. Most patients were Caucasian males with an average age of 71.6 years. The median follow‐up time was 29.4 months.Thirty‐six percent of patients had pathological evidence of cervical metastasis (N+). Seven percent had metastasis to level I, 21.9% to level II, 14.6% to level III, 8% to level IV, and 9.5% to level V. Thirty‐nine percent of patients had metastasis to parotid region (P+). The average number of positive:total nodes from parotidectomy was 1.9:4.6.The median overall and disease‐specific survival times were 2.81 and 1.96 years, respectively. Patients with either neck or parotid metastasis (N + or P+) had significantly decreased survival, hazard ratio 2.298 (1.2739, 4.1445), compared to patients without metastasis (N0P0), P < 0.0057.ConclusionPatients with periauricular region cSCC metastasizing to parotid and cervical regions lymph nodes have significantly decreased survival compared with patients without cervical and/or parotid metastasis. Metastasis to parotid region should be considered as a distinct head and neck level when staging periauricular cancers. This study provides a basis for further studies to validate these findings.Level of Evidence4Laryngoscope, 129:1579–1586, 2019
Cellular and molecular mechanisms of cleft palate development
Authors: Deshpande Anita S. , Goudy Steven L. 
Published: November 2018

AbstractCleft lip and palate are common craniofacial deformities. The etiology underlying these deformities is complex and multifactorial and they can occur as part of one of many chromosomal syndromes, Mendelian single gene disorders, teratogenic effects, and as yet uncharacterized syndromes. Our paper will provide an overview of the multiple genes and molecular pathways that have been implicated in palatal fusion. We believe that understanding the molecular mechanisms of cleft formation can help clinicians anticipate which patients may have difficulties healing and in the future allow them to make surgical and medical treatment decisions based on genetic information.
Colored organic matter increases CO2 in meso‐eutrophic lake water through altered light climate and acidity
Authors: Nydahl Anna C. , Wallin Marcus B. , Tranvik Lars J. , Hiller Carolin , Attermeyer Katrin , Garrison Julie A. , Chaguaceda Fernando , Scharnweber Kristin , Weyhenmeyer Gesa A. 
Published: November 2018

AbstractMany surface waters across the boreal region are browning due to increased concentrations of colored allochthonous dissolved organic carbon (DOC). Browning may stimulate heterotrophic metabolism, may have a shading effect constraining primary production, and may acidify the water leading to decreased pH with a subsequent shift in the carbonate system. All these effects are expected to result in increased lake water carbon dioxide (CO2) concentrations. We tested here these expectations by assessing the effects of both altered allochthonous DOC input and light conditions through shading on lake water CO2 concentrations. We used two mesocosm experiments with water from the meso‐eutrophic Lake Erken, Sweden, to determine the relative importance of bacterial activities, primary production, and shifts in the carbonate system on CO2 concentrations. We found that DOC addition and shading resulted in a significant increase in partial pressure of CO2 (pCO2) in all mesocosms. Surprisingly, there was no relationship between bacterial activities and pCO2. Instead the experimental reduction of light by DOC and/or shading decreased the photosynthesis to respiration ratio leading to increased pCO2. Another driving force behind the observed pCO2 increase was a significant decrease in pH, caused by a decline in photosynthesis and the input of acidic DOC. Considering that colored allochthonous DOC may increase in a warmer and wetter climate, our results could also apply for whole lake ecosystems and pCO2 may increase in many lakes through a reduction in the rate of photosynthesis and decreased pH.
Induction of defensive traits in marine plankton—new copepodamide structures
Authors: Grebner Wiebke , Berglund E. Carina , Berggren Fredrik , Eklund Johan , Harðadóttir Sara , Andersson Mats X. , Selander Erik 
Published: November 2018

AbstractMarine zooplankton release chemical cues, which trigger defenses in unicellular phytoplankton, such as increased toxin production and changes of colony sizes. Here, we identify the structure of two novel alarm cues belonging to the group of copepodamides. Similar to the known copepodamides, one of the compounds described is shown to trigger both amnesic and paralytic shellfish toxin production and chain length shortening in Skeletonema marinoi. In addition, we report the putative structures of another 21 copepodamides, which constitute 28% of the total copepodamides extractable from whole animals, suggesting that the copepodamide concentrations have been underestimated in earlier studies. We introduce a structure‐based nomenclature to handle the increasing number of copepodamides. Analysis of 12 copepod species showed that marine calanoid and freshwater cyclopoid copepods contain copepodamides. The only harpacticoid included in the analysis, Tigriopus californicus, did not appear to produce detectable amounts of copepodamides. Feeding experiments revealed that copepodamide compositions depend on both diet and species‐specific properties. Copepodamides induce both morphological and biochemical defensive traits in phytoplankton and may drive large‐scale trait–mediated effects in marine food webs. The more comprehensive list of copepodamides reported here makes it possible to explore the role of the copepodamide signaling system in the pelagic ecosystem in greater detail.
Processing of Cellulose Using Ionic Liquids
Authors: Hermanutz Frank , Vocht Marc Philip , Panzier Nicole , Buchmeiser Michael R. 
Published: November 2018

AbstractThe processing of cellulose dissolved in ionic liquids (ILs) enables the development of new materials. Besides the established production of cellulosic fiber products, interesting technical applications are developed like super micro filament fibers, cellulose/chitin blend fibers, precursors for carbon fibers, and all‐cellulose composites. This review provides a detailed summary of these new developments and describes how ILs are selected for the processing of cellulose with a particular emphasis on industrial realization. State‐of‐the‐art spinning processes are reviewed and it is illustrated how uniquely selected ILs can be used not only for established fiber spinning but for the development of new cellulose‐based materials.
Silane‐Crosslinked Asymmetric Polythiosemicarbazide Membranes for Organic Solvent Nanofiltration
Authors: Aburabie Jamaliah , Emwas Abdul‐Hamid , Peinemann Klaus‐Viktor 
Published: November 2018

AbstractCrosslinked polythiosemicarbazide (PTSC) membranes with a positively charged surface are fabricated via a reaction with (3‐glycidyloxypropyl)trimethoxysilane. The integrally asymmetric ultrafiltration membranes discussed here can be easily prepared by water‐induced phase separation using a PTSC solution in dimethylsulfoxide (DMSO). The crosslinked PTSC membranes are stable in DMSO, N,N‐dimethylformamide, and tetrahydrofuran and they reject molecules of molecular weights (MW) above 1300 g mol−1. The influence of the crosslinking agent on the surface charge, membrane solvent resistance, and membrane performance is discussed. The crosslinked asymmetric PTSC membranes totally reject Direct Red dye (MW 1373 g mol−1), while the pristine PTSC membrane does not show any rejection for this dye. This finding suggests that an inorganic‐type‐network is formed during the crosslinking reaction, which tunes the pore size of the prepared membranes.
Orientation Distribution Function Pattern for Rigid Dumbbell Suspensions in Any Simple Shear Flow
Authors: Jbara Layal M. , Giacomin Alan Jeffrey 
Published: November 2018

AbstractFor rigid dumbbells suspended in a Newtonian solvent, the viscoelastic response depends exclusively on the dynamics of dumbbell orientation. The orientation distribution function ψ(θ, φ, t) represents the probability of finding dumbbells within the range (θ, θ + dθ) and (φ, φ + dφ). This function is expressed in terms of a partial differential equation (the diffusion equation), which, for any simple shear flow, is solved by postulating a series expansion in the shear rate magnitude. Each order of this expansion yields a new partial differential equation, for which one must postulate a form for its solution. This paper finds a simple and direct pattern to these solutions. The use of this pattern reduces the amount of work required to determine the coefficients of the power series expansion of the orientation distribution function, ψ
i
. To demonstrate the usefulness of this new pattern, new expressions for these coefficients are derived up to and including the sixth power of the shear rate magnitude. This work also completes previous findings that ended at the fourth power of the shear rate magnitude.
Immunogenicity of a bivalent protein as a vaccine against Edwardsiella anguillarum and Vibrio vulnificus in Japanese eel (Anguilla japonica)
Authors: Guo Songlin , Hu Linlin , Feng Jianjun , Lin Peng , He Le , Yan Qingpi 
Published: November 2018

AbstractThe OMPs A (OmpA)—of Edwardsiella anguillarum and OmpU of V. vulnificus have been proven to be good antigens. In this study, after construction of a vector, a new recombinant Omp (rOMP) containing both OmpA and OmpU was expressed and purified. Then, the Japanese eels (Anguilla japonica) were intraperitoneally (i.p.) injected with the phosphate‐buffered saline (PBS group), formalin‐killed‐cell (FKC group) or the recombinant Omp (rOMP group). The stimulation index of the whole blood cells in eels from FKC group was significantly higher than the eels from PBS and rOMP groups at 28 dpi; serum titers of anti‐E. anguillarum and anti‐V. vulnificus antibody of eels from FKC and rOMP group increased significantly at 21 and 28 dpi; in the rOMP group, eels serum titer stayed at a high level on 42 dpi. The activities of lysozyme in skin mucus, liver, kidney, and serum in three groups exhibited considerable changes. The relative percent survival (RPS) rate of eels from rOMP group were 100% and 83% when challenged with V. vulnificus or E. anguillarum. These results indicated that inoculation of rOMP would protect Japanese eels against the infection by E. anguillarum and V. vulnificus.
Pressure and temperature effects on deep‐sea hydrocarbon‐degrading microbial communities in subarctic sediments
Authors: Perez Calderon Luis J. , Gontikaki Evangelia , Potts Lloyd D. , Shaw Sophie , Gallego Alejandro , Anderson James A. , Witte Ursula 
Published: November 2018

AbstractThe Hatton–Rockall Basin (North‐East Atlantic) is an area with potential for deep‐sea (2,900 m) hydrocarbon exploration. Following the Deepwater Horizon oil spill, many investigations into the responses of sediment microbial communities to oil pollution have been undertaken. However, hydrostatic pressure is a parameter that is often omitted due to the technical difficulties associated with conducting experiments at high pressure (>10 MPa). In this study, sediments from 2,900 m in the Hatton–Rockall Basin, following a one‐week decompression period in a temperature‐controlled room at 5°C, were incubated in factorial combinations of 0.1 and 30 MPa, 5 and 20°C, and contamination with a hydrocarbon mixture or uncontaminated controls to evaluate the effect of these environmental variables on the bacterial community composition. Our results revealed varying effects of pressure, temperature, and oil contamination on the composition of the bacterial community within the sediment. Temperature was the strongest determinant of differences in the bacterial community structure between samples followed by pressure. Oil contamination did not exert a strong change in the sediment bacterial community structure when pressure and temperature conditions were held at in situ levels (30 MPa and 5°C). The γ‐proteobacteria Pseudomonas and Colwellia, and several Bacteroidetes dominated communities at 30 MPa. In contrast, hydrocarbon degraders such as Halomonas, Alcanivorax, and Marinobacter decreased in relative abundance at the same pressure. This study highlights the importance of considering hydrostatic pressure in ex situ investigations into hydrocarbon‐degrading deepwater microbial communities.
Suggestive association between OPRM1 and impulse control disorders in Parkinson's disease
Authors: Cormier‐Dequaire Florence , Bekadar Samir , Anheim Mathieu , Lebbah Said , Pelissolo Antoine , Krack Paul , Lacomblez Lucette , Lhommée Eugénie , Castrioto Anna , Azulay Jean‐Philippe , Defebvre Luc , Kreisler Alexandre , Durif Franck , Marques‐Raquel Ana , Brefel‐Courbon Christine , Grabli David , Roze Emmanuel , Llorca Pierre‐Michel , Ory‐Magne Fabienne , Benatru Isabelle , Ansquer Solene , Maltête David , Tir Melissa , Krystkowiak Pierre , Tranchant Christine , Lagha‐Boukbiza Ouhaid , Lebrun‐Vignes Bénédicte , Mangone Graziella , Vidailhet Marie , Charbonnier‐Beaupel Fanny , Rascol Olivier , Lesage Suzanne , Brice Alexis , Tezenas du Montcel Sophie , Corvol Jean‐Christophe ,   the BADGE‐PD study group
Published: November 2018

ABSTRACTBackgroundImpulse control disorders are frequently associated with dopaminergic therapy in Parkinson's disease. Genetic studies have suggested a high heritability of impulse control disorders in the general population and in PD. The aim of this study was to identify candidate gene variants associated with impulse control disorders and related behaviors in PD.MethodsWe performed a multicenter case‐control study in PD patients with (cases) or without impulse control disorders and related behaviors despite significant dopamine agonist exposure of >300 mg levodopa‐equivalent daily dose during 12 months (controls). Behavioral disorders were assessed using the Ardouin scale. We investigated 50 variants in 24 candidate genes by a multivariate logistic regression analysis adjusted for sex and age at PD onset.ResultsThe analysis was performed on 172 cases and 132 controls. Cases were younger (60 ± 8 vs 63 ± 8 years; P < 0.001) and had a higher family history of pathological gambling (12% vs 5%, P = 0.03). No variant was significantly associated with impulse control disorders or related behaviors after correction for multiple testing, although the 2 top variants were close to significant (OPRM1 rs179991, OR, 0.49; 95%CI, 0.32‐0.76; P = 0.0013; Bonferroni adjusted P = 0.065; DAT1 40‐base pair variable number tandem repeat, OR, 1.82; 95%CI, 1.24‐2.68; P = 0.0021; Bonferroni adjusted P = 0.105).ConclusionsOur results are suggestive of a novel association of the opioid receptor gene OPRM1 with impulse control disorders and related behaviors in PD and confirm a previous association with DAT1. Although replication in independent studies is needed, our results bring potential new insights to the understanding of molecular mechanisms of impulse control disorders. © 2018 International Parkinson and Movement Disorder Society