bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new pattern of underground space‐time tridimensional gas drainage: A case study in Yuwu coal mine, China
Authors: Li Yankui , Wu Shiyue , Nie Baisheng , Ma Yankun 
Published: February 2019

AbstractWith the increase of mining depth, the high gassy coal mine confronts with a variety of gas problems, such as gas concentration exceeding the limit in the working face, gas outburst and gas explosion. According to the situation of gas drainage in Qinshui Basin (South of Shanxi Province, China) and enlightened by horizontal directional drillings (HDD) and multi‐branched horizontal wells, a new gas drainage pattern that invertedπ‐shape km borehole layout was proposed. Verified in Yuwu Coal Mine, the gas drainage data were standardized via the unified theory of standardization. It could dropped the coal seam gas content to 7.71 m3/t, and increased the gas drainage rate to 34.2%, which conformed to the Chinese regulation (<8 m3/t, >30%, respectively). This pattern has a significant effect on coal mines with a single seam, where protective seam mining technology was unsuitable to prevent gas outburst and gas explosion.
Fire performance of closed‐cell charring insulation materials in plasterboard‐insulation assemblies
Authors: Hidalgo Juan P. , Torero José L. , Welch Stephen 
Published: February 2019

SummaryThis paper presents an experimental study on the fire performance of two types of plastic charring insulation materials when covered by a plasterboard lining. The specific insulation materials correspond to rigid closed‐cell plastic foams, a type of polyisocyanurate (foam A) and a type of phenolic foam (foam B), whose thermal decomposition and flammability were characterised in previous studies. The assemblies were instrumented with thermocouples. The plasterboard facing was subjected to constant levels of irradiation of 15, 25, and 65 kW m−2 using the heat‐transfer rate inducing system. These experiments serve as (1) an assessment of the fire behaviour of these materials studied at the assembly scale and (2) an identification of the fire hazards that these systems pose in building construction. The manifestation of the hazards occurred via initial pyrolysis reactions and release of volatiles followed by various complex behaviours including char oxidation (smouldering), cracking, and expansion of the foam. Gas‐phase conditions may support ignition of the volatiles, sustained burning, and ultimately spread of the flame through the unexposed insulation face. The results presented herein are used to validate the insulation “critical temperature” concept used for a performance‐based methodology focused on the selection of suitable thermal barriers for flammable insulation.
Discontinuous Galerkin finite element method applied to the coupled unsteady Stokes/Cahn‐Hilliard equations
Authors: Pigeonneau F. , Hachem E. , Saramito P. 
Published: February 2019

SummaryTwo‐phase flows driven by the interfacial dynamics are studied by tracking implicitly interfaces in the framework of the Cahn‐Hilliard theory. The fluid dynamics is described by the Stokes equations with an additional source term in the momentum equation taking into account the capillary forces. A discontinuous Galerkin finite element method is used to solve the coupled Stokes/Cahn‐Hilliard equations. The Cahn‐Hilliard equation is treated as a system of two coupled equations corresponding to the advection‐diffusion equation for the phase field and a nonlinear elliptic equation for the chemical potential. First, the variational formulation of the Cahn‐Hilliard equation is presented. A numerical test is achieved showing the optimal order in error bounds. Second, the variational formulation in discontinuous Galerkin finite element approach of the Stokes equations is recalled, in which the same space of approximation is used for the velocity and the pressure with an adequate stabilization technique. The rates of convergence in space and time are evaluated leading to an optimal order in error bounds in space and a second order in time with a backward differentiation formula at the second order. Numerical tests devoted to two‐phase flows are provided on ellipsoidal droplet retraction, on the capillary rising of a liquid in a tube, and on the wetting drop over a horizontal solid wall.
The impacts of public news announcements on intraday implied volatility dynamics
Authors: Lee Jieun , Ryu Doojin 
Published: February 2019

AbstractWe examine the responses of intraday option‐implied volatilities to scheduled announcements of macroeconomic indicators. The increase in implied volatility around macroeconomic news announcements is more pronounced for puts than for calls and is stronger for announcements made during trading hours than for those made during nontrading hours. These effects are also more pronounced in the crisis and postcrisis periods than in the precrisis period. Monetary policy announcements have a more substantial impact on volatility than other announcements have, even after controlling for news surprise components. The impact appears to be greater for policy rate hikes than for policy rate cuts.
HPV as a marker for molecular characterization in head and neck oncology: Looking for a standardization of clinical use and of detection method(s) in clinical practice
Authors: Bussu Francesco , Ragin Camille , Boscolo‐Rizzo Paolo , Rizzo Davide , Gallus Roberto , Delogu Giovanni , Morbini Patrizia , Tommasino Massimo 
Published: February 2019

AbstractBackgroundA consensus about the most appropriate diagnostic method(s) for head and neck human papillomavirus (HPV)‐induced carcinogenesis is still lacking because most of the commercially available assays have been designed for the cervix.MethodsThis article summarizes current data and trends concerning HPV diagnostic strategies in oropharyngeal squamous cell carcinoma (OPSCC). Six main approaches are described.ResultsThe diagnostic gold standard for HPV‐related OPSCC, focusing on E6/E7 mRNA detection, requires fresh samples. Because most frequently available samples are formalin‐fixed paraffin‐embedded (FFPE), the pros and cons of the different approaches were analyzed.ConclusionsIn the FFPE samples, the immunohistochemistry of p16, which is considered appropriate to assess HPV‐driven carcinogenesis in OPSCC according to the 8th American Joint Committee on Cancer TNM classification, may not be specific enough to become the diagnostic standard in the perspective of treatment deintensification. p16 may play a safer role in combination with another highly sensible assay. Other promising approaches are based on DNA detection through real‐time polymerase chain reaction and RNAscope.
Role of Akirin1 in the regulation of skeletal muscle fiber‐type switch
Authors: Rao Vanitha Venkoba , Sangiah Umamaheswari , Mary Kavitha Arockia , Akira Shizuo , Mohanty Abhishek 
Published: February 2019

AbstractAkirin1 is a highly conserved ubiquitously expressed nuclear protein. Owing to its strong nuclear localization signal and protein‐protein interaction properties, Akirin1 has been speculated to regulate transcription of target genes as a cofactor. Previous studies have reported Akirin1 as a downstream target of myostatin, a potent negative regulator of myogenesis. Mice lacking myostatin displayed enhanced Akirin1 gene expression. Further, in vitro evidence has shown Akirin1 overexpression leads to hypertrophy in C2C
12 myotubes. In this study, we used Akirin1 knockout mice as a model system to further elucidate the function of Akirin1 in fully differentiated skeletal muscle. Akirin1 knockout mice did not show any obvious phenotypic difference when compared with wild type. However, promoter‐reporter assay suggested that Akirin1 regulated the transcription of muscle‐specific RING finger 1 (MuRF‐1), an important E3 ubiquitin ligase in skeletal muscle. Furthermore, ablation of Akirin1 resulted in increased type IIa and decreased type I muscle fibers, which was further supported by an increase in Myh2 and decrease in Myh7 gene expression. Also, histochemical studies for succinate dehydrogenase activity revealed a less oxidative muscle in the absence of Akirin1. Together, our study suggests a novel role of Akirin1 in maintaining the muscle fiber type and regulation of the metabolic activity of the skeletal muscle.
Identifying novel biomarkers in hepatocellular carcinoma by weighted gene co‐expression network analysis
Authors: Li Boxuan , Pu Ke , Wu Xinan 
Published: February 2019

AbstractHepatocellular carcinoma (HCC) is a highly malignant tumor found in the bile duct epithelial cells, and the second most common tumor of the liver. However, the pivotal roles of most molecules of tumorigenesis in HCC are still unclear. Hence, it is essential to detect the tumorigenic mechanism and develop novel prognostic biomarkers for clinical application. The data of HCC mRNA‐seq and clinical information from The Cancer Genome Atlas (TCGA) database were analyzed by weighted gene co‐expression network analysis (WGCNA). Co‐expression modules and clinical traits were constructed by the Pearson correlation analysis, interesting modules were selected and gene ontology and pathway enrichment analysis were performed. Intramodule analysis and protein–protein interaction construction of selected modules were conducted to screen hub genes. In addition, upstream transcription factors and microRNAs of hub genes were predicted by miRecords and NetworkAnalyst database. Afterward, a high connectivity degree of hub genes from two networks was picked out to perform the differential expression validation in the Gene Expression Profiling Interactive Analysis database and Human Protein Atlas database and survival analysis in Kaplan–Meier plotter online tool. By utilizing WGCNA, several hub genes that regulate the mechanism of tumorigenesis in HCC were identified, which was associated with clinical traits including the pathological stage, histological grade, and liver function. Surprisingly, ZWINT, CENPA, RACGAP1, PLK1, NCAPG, OIP5, CDCA8, PRC1, and CDK1 were identified statistically as hub genes in the blue module, which were closely implicated in pathological T stage and histologic grade of HCC. Moreover, these genes also were strongly associated with the HCC cell growth and division. Network and survival analyses found that nine hub genes may be considered theoretically as indicators to predict the prognosis of patients with HCC or clinical treatment target, it will be necessary for basic experiments and large‐scale cohort studies to validate further.
Molecular docking, dynamics, and pharmacology studies on bexarotene as an agonist of ligand‐activated transcription factors, retinoid X receptors
Authors: Chitranshi Nitin , Dheer Yogita , Kumar Sanjay , Graham Stuart L. , Gupta Vivek 
Published: February 2019

AbstractRetinoid X receptors (RXRs) belong to the nuclear receptor superfamily, and upon ligand activation, these receptors control gene transcription via either homodimerization with themselves or heterodimerization with the partner‐nuclear receptor. The protective effects of RXRs and RXR agonists have been reported in several neurodegenerative diseases, including in the retina. This study was aimed to prioritize compounds from natural and synthetic origin retinoids as potential RXR agonists by molecular docking and molecular dynamic simulation strategies. The docking studies indicated bexarotene as a lead compound that can activate various RXR receptor isoforms (α, β, and γ) and has a strong binding affinity to the receptor protein than retinoic acid, which is known as a natural endogenous RXR agonist. Dynamic simulation studies confirmed that the hydrogen bonding and hydrophobic interactions were highly stable in all the three isoforms of the RXR‐bexarotene complex. To further validate the significance of the RXR receptor in neurons, in vitro pharmacological treatment of neuronal SH‐SY5Y cells with bexarotene was performed. In vitro data from SH‐SY5Y cells confirmed that bexarotene activated RXR‐simulated neurite outgrowth significantly. We conclude that bexarotene could be potentially used as an exogenous activator of RXRs and emerge as a good drug target for several neurodegenerative disorders.
Effect of cell source and osteoblast differentiation on gene expression profiles of mesenchymal stem cells derived from bone marrow or adipose tissue
Authors: Fideles Simone Ortiz Moura , Ortiz Adriana Cassia , Assis Amanda Freire , Duarte Max Jordan , Oliveira Fabiola Singaretti , Passos Geraldo Aleixo , Beloti Márcio Mateus , Rosa Adalberto Luiz 
Published: February 2019

AbstractMesenchymal stem cells (MSCs) have been used in therapies for bone tissue healing. The aim of this study was to investigate the effect of cell source and osteoblast differentiation on gene expression profiles of MSCs from bone marrow (BM‐MSCs) or adipose tissue (AT‐MSCs) to contribute for selecting a suitable cell population to be used in cell‐based strategies for bone regeneration. BM‐MSCs and AT‐MSCs were cultured in growth medium to keep MSCs characteristics or in osteogenic medium to induce osteoblast differentiation (BM‐OBs and AT‐OBs). The transcriptomic analysis was performed by microarray covering the entire rat functional genome. It was observed that cells from bone marrow presented higher expression of genes related to osteogenesis, whereas cells from adipose tissue showed a higher expression of genes related to angiogenesis and adipocyte differentiation, irrespective of cell differentiation. By comparing cells from the same source, MSCs from both sources exhibited higher expression of genes involved in angiogenesis, osteoblast differentiation, and bone morphogenesis than osteoblasts. The clustering analysis showed that AT‐OBs exhibited a gene expression profile closer to MSCs from both sources than BM‐OBs, suggesting that BM‐OBs were in a more advanced stage of differentiation. In conclusion, our results suggest that in cell‐based therapies for bone regeneration AT‐MSCs could be considered for angiogenic purposes, whereas BM‐MSCs and osteoblasts differentiated from either source could be better for osteogenic approaches.
Thioredoxin‐interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy
Authors: Song Shan , Qiu Duojun , Shi Yonghong , Wang Shuai , Zhou Xinbo , Chen Nan , Wei Jinying , Wu Ming , Wu Haijiang , Duan Huijun 
Published: February 2019

AbstractThioredoxin‐interacting protein (TXNIP) is induced by high glucose (HG), whereupon it acts to inhibit thioredoxin, thereby promoting oxidative stress. We have found that TXNIP knockdown in human renal tubular cells helped prevent the epithelial‐to‐mesenchymal transition (EMT). Here, we studied the potential effect of TXNIP on podocyte phenotypic alterations in diabetic nephropathy (DN) in vivo and in vitro. In conditionally immortalized mouse podocytes under HG conditions, knocking down TXNIP disrupted EMT, reactive oxygen species (ROS) production, and mammalian target of rapamycin (mTOR) pathway activation. Further, Raptor short hairpin RNA (shRNA), Rictor shRNA, and mTOR specific inhibitor KU‐0063794 were used to assess if the mTOR signal pathway is involved in HG‐induced EMT in podocytes. We found that Raptor shRNA, Rictor shRNA, and KU‐0063794 could all restrain HG‐induced EMT and ROS production in podocytes. In addition, antioxidant Tempol or N‐acetylcysteine presented a prohibitive effect on HG‐induced EMT in podocytes. Streptozotocin was utilized to render equally diabetic in wild‐type (WT) control and TXNIP
−/− (TKO) mice. Diabetes did not increase levels of 24‐hr urinary protein, serum creatinine, blood urea nitrogen, and triglyceride in TXNIP
−/− mice. Podocyte phenotypic alterations and podocyte loss were detected in WT but not in TKO diabetic mice. Oxidative stress was also suppressed in diabetic TKO mice relative to WT controls. Also, TXNIP deficiency suppresses the activation of mTOR in glomeruli of streptozotocin‐induced diabetic mice. Moreover, TXNIP expression, mTOR activation, Nox1, and Nox4 could be detected in renal biopsy tissues of patients with DN. This suggests that decreased TXNIP could ameliorate phenotypic alterations of podocytes via inhibition of mTOR in DN, highlighting TXNIP as a promising therapeutic target.
Allogeneic hematopoietic stem cell transplantation with myeloablative conditioning for adult cerebral X‐linked adrenoleukodystrophy
Authors: Waldhüter Nils , Köhler Wolfgang , Hemmati Philipp G. , Jehn Christian , Peceny Rudolf , Vuong Giang L. , Arnold Renate , Kühl Jörn‐Sven 
Published: February 2019

AbstractThe adult cerebral form of X‐linked adrenoleukodystrophy (ACALD), an acute inflammatory demyelinating disease, results in a rapidly progressive neurodegeneration, typically leading to severe disability or death within a few years after onset. We have treated 15 men who had developed ACALD with allogeneic hematopoietic stem cell transplantation (HSCT) from matched donors after myeloablative conditioning with busulfan and cyclophosphamide. All patients engrafted and 11 survived (estimated survival 73 ± 11%), 8 with stable cognition and 7 of them with stable motor function (estimated event‐free survival 36 ± 17%). Death after transplantation occurred within the first year after HSCT and was caused either primarily by infection (N = 3) or due to disease progression triggered by infection (N = 1). Patients with minor myelopathic symptoms (N = 4) or with no or mild cerebral symptoms pre‐transplant (N = 7) had an excellent outcome. In contrast, no patient with major neurological symptoms associated with an extensive involvement of pyramidal tract fibres in the internal capsule (N = 5) survived without cognitive deterioration. Notably, early leukocyte recovery was associated with dismal outcome for yet unknown reasons. All 10 tested survivors showed a reduction of plasma hexacosanoic acid (C26:0) in the absence of Lorenzo's oil. Over time, the event‐free survival could be improved from 2 out of 8 patients (25%) before 2013 to 5 out of 7 patients (71%) thereafter. Therefore, allogeneic HSCT appears to be a suitable treatment option for carefully selected ACALD patients when transplanted from matched donors after myeloablative, busulfan‐based conditioning.
IFN-γ-dependent nitric oxide suppresses Brucella-induced arthritis by inhibition of inflammasome activation
Authors: Lacey Carolyn A , Chambers Catherine A , Mitchell William J , Skyberg Jerod A 
Published: February 2019

Abstract
Brucellosis, caused by the intracellular bacterial pathogen Brucella, is a globally important zoonotic disease for which arthritis is the most common focal complication in humans. Wild-type mice infected systemically with Brucella typically do not exhibit arthritis, but mice lacking IFN-γ develop arthritis regardless of the route of Brucella infection. Here, we investigated mechanisms by which IFN-γ suppresses Brucella-induced arthritis. Several cell types, including innate lymphoid cells, contributed to IFN-γ production and suppression of joint swelling. IFN-γ deficiency resulted in elevated joint IL-1β levels, and severe joint inflammation that was entirely inflammasome dependent, and in particular, reliant on the NLRP3 inflammasome. IFN-γ was vital for induction of the nitric oxide producing enzyme, iNOS, in infected joints, and nitric oxide directly inhibited IL-1β production and inflammasome activation in Brucella-infected macrophages in vitro. During in vivo infection, iNOS deficiency resulted in an increase in IL-1β and inflammation in Brucella-infected joints. Collectively, this data indicate that IFN-γ prevents arthritis both by limiting Brucella infection, and by inhibiting excessive inflammasome activation through the induction of nitric oxide.
Perioperative outcomes of extensive bowel resection during cytoreductive surgery in patients with advanced ovarian cancer
Authors: Son Joo‐Hyuk , Kong Tae‐Wook , Paek Jiheum , Chang Suk‐Joon , Ryu Hee‐Sug 
Published: February 2019

AbstractBackground and ObjectivesTo achieve optimal cytoreduction, extensive bowel resections are sometimes required in patients with advanced ovarian cancer. Few studies have focused on the extent or number of resections of bowel surgeries and their feasibility.MethodsWe retrospectively reviewed the medical records of patients with advanced ovarian cancer who underwent bowel surgery as part of debulking procedures at Ajou University Hospital from 2006 to 2018. Patients who received extensive bowel resections (two‐segment resections or subtotal colectomy) were identified, and their perioperative outcomes were evaluated.ResultsA total of 172 patients underwent bowel surgery. Of them, 128 (74.4%) underwent one‐segment bowel resection, 25 (14.5%) underwent two‐segment bowel resections, and 19 (11.1%) underwent subtotal colectomy. Although the operative time, transfusion rate, and postoperative bleeding events were higher in patients who underwent extensive bowel resection, the rates of perioperative complications were not significantly higher in this group. Anastomotic leakage occurred in two (1.5%) patients in the one‐segment resection group, one (4.2%) patient in the multiple resection group, and two (10.5%) patients in the subtotal colectomy group.ConclusionsMultiple bowel resections (up to two segments) are feasible and can be safely performed with an acceptable complication rate in patients with advanced ovarian cancer.
Factors of success of low‐dose macrolides in chronic sinusitis: Systematic review and meta‐analysis
Authors: Seresirikachorn Kachorn , Suwanparin Nichana , Srisunthornphanich Chanyanuch , Chitsuthipakorn Wirach , Kanjanawasee Dichapong , Snidvongs Kornkiat 
Published: February 2019

ObjectiveTo assess the prognostic factors that predict favorable outcomes of low‐dose macrolides (LDMs) in treating chronic rhinosinusitis (CRS).MethodsRandomized controlled trials studying the effects of LDMs in treating CRS were included. Data were pooled for meta‐analysis. Primary outcome was Sino‐Nasal Outcome Test (SNOT). Six prognostic factors: CRS subtypes, serum immunoglobulin (Ig)E level, membered lactone ring of macrolides, concurrent endoscopic sinus surgery (ESS), and dosage and duration of the LDMs were assessed by subgroup analyses.ResultsTen studies (608 patients) met the inclusion criteria. LDMs and placebo were not different in SNOT improvement (standardized mean difference [SMD] = −0.23, 95% confidence interval [CI]: −0.69 to 0.24). Subgroup analyses showed that the effects favored LDMs in the patients with CRS without polyps (SMD = −0.64, 95% CI: −1.01 to −0.27) compared to CRS with polyps, and the patients receiving a half dose (SMD = −0.64, 95% CI: −1.01 to −0.27) compared to a very low dose. There was no difference in SNOT improvement between LDMs plus standard treatment compared to standard treatment (SMD = −0.52, 95% CI: −1.57 to 0.53). Subgroup analyses showed that the effects favored LDMs in the patients receiving LDMs for a duration of 24 weeks (SMD = −1.68, 95% CI: −2.40 to −0.95) compared to 8 and 12 weeks. There was no difference between the 14‐membered and 15‐membered ring LDMs. Assessment of concurrent ESS found mixed results. Serum IgE level could not be assessed.ConclusionsLDMs provided favorable outcomes in patients with CRS without polyps. A half dose of macrolides should be given for a duration of 24 weeks.Level of Evidence1aLaryngoscope, 129:1510–1519, 2019