bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial genome reorganization characterizes various lineages of mesostigmatid mites (Acari: Parasitiformes)
Authors: Li Wei‐Ning , Shao Renfu , Zhang Qi , Deng Wei , Xue Xiao‐Feng 
Published: July 2019

AbstractMesostigmata is an extremely diverse group of mites with more than 11,000 described species in 109 families. The complete mitochondrial (mt) genomes of five species of mesostigmatid mites from three families (Varroidae, Ologamasidae, Phytoseiidae) have been reported previously; all of them are rearranged or highly rearranged in gene order. However, it is unclear when mt genome reorganization occurred and how common it is in mesostigmatid mites. We sequenced the mt genomes of ten species of mesostigmatid mites from five more families (Blattisociidae, Diplogyniidae, Laelapidae, Macrochelidae, Parasitidae). We found that species in the families Diplogyniidae and Parasitidae have retained the ancestral mt genome organization of arthropods, which is in stark contrast to the highly rearranged mt genomes in the Phytoseiidae species. As in the Varroidae and Ologamasidae species, the mt genomes of the Blattisociidae, Macrochelidae and Laelapidae species are also rearranged but are less rearranged than in the Phytoseiidae species. Each of the six mesostigmatid families that have rearranged mt genomes is characterized by unique gene order not seen in other mesostigmatid families. Furthermore, the mt genome organization also differs among three genera of the Phytoseiidae, between two genera of the Laelapidae, and among threeMacrochelesspecies of the Macrochelidae. Our results indicate that: (a) the most recent common ancestor of mesostigmatid mites likely retained the ancestral mt genome organization of arthropods; and (b) mt genome organization characterizes various lineages of mesostigmatid mites and provides a valuable source of information for understanding their phylogeny and evolution.
Do European Union countries adequately address the healthcare needs of adolescents in the area of sexual reproductive health and rights?
Authors: Michaud Pierre-André , Visser Annemieke , Vervoort Johanna , Kocken Paul , Reijneveld Sijmen , Blair Mitch , Alexander Denise , Rigby Michael , Weber Martin , Jansen Danielle 
Published: July 2019

Background and objectivesAdolescent sexual and reproductive health and rights (SRHR) are of particular relevance given their potential short-term or long-term health consequences. This study evaluates recommendations and policies regarding access to care in this area in 31 European countries (European Union (EU) plus Iceland, Norway and Switzerland).MethodsAs part of the EU funded Models of Child Health Appraised project, data were gathered using a 43-item questionnaire sent to experts responsible for collecting information in each country.ResultsTen countries have not developed any formal policy or recommendation that guarantee the respect of confidentiality and the possibility of consulting a physician without parents knowing. Nearly half of the countries do not have centres specialised in adolescent healthcare, tackling comprehensive health issues or focusing specifically on SRH. Access to emergency contraception and information regarding pregnancy, including testing, is easy in most countries. However, oral contraception is delivered free of charge in only 10 countries. Twenty-three countries do not meet current standards in terms of providing policy-based pregnancy care, and only 13 have set up special programmes for pregnant adolescents. In only seven countries can adolescents definitely have their pregnancy terminated without their parents knowing (and in another seven countries in selected situations).ConclusionThe provision and availability of adolescent-friendly SRHR care are far from optimal in around half of the surveyed countries. These results call for the review and implementation of policies, specialised healthcare centres and training initiatives for primary care providers.
Effect of medical student debt on mental health, academic performance and specialty choice: a systematic review
Authors: Pisaniello Monique Simone , Asahina Adon Toru , Bacchi Stephen , Wagner Morganne , Perry Seth W , Wong Ma-Li , Licinio Julio 
Published: July 2019

ObjectivesWith the high and rising total cost of medical school, medical student debt is an increasing concern for medical students and graduates, with significant potential to impact the well-being of physicians and their patients. We hypothesised that medical student debt levels would be negatively correlated with mental health and academic performance, and would influence career direction (ie, medical specialty choice).DesignWe performed a systematic literature review to identify articles that assessed associations between medical student mental health, academic performance, specialty choice and debt. The databases PubMed, Medline, Embase, Scopus and PsycINFO were searched on 12 April 2017, for combinations of the medical subject headingsMedical StudentandDebtas search terms. Updates were incorporated on 24 April 2019.Results678 articles were identified, of which 52 met the inclusion criteria after being reviewed in full text. The majority of studies were conducted in the USA with some from Canada, New Zealand, Scotland and Australia. The most heavily researched aspect was the association between medical student debt and specialty choice, with the majority of studies finding that medical student debt was associated with pursuit of higher paying specialties. In addition, reported levels of financial stress were high among medical students, and correlated with debt. Finally, debt was also shown to be associated with poorer academic performance.ConclusionsMedical student debt levels are negatively associated with mental well-being and academic outcomes, and high debt is likely to drive students towards choosing higher paying specialties. Additional prospective studies may be warranted, to better understand how educational debt loads are affecting the well-being, career preparation and career choices of physicians-in-training, which may in turn impact the quality of care provided to their current and future patients.
‘This is our liver patient…’: use of narratives during resident and nurse handoff conversations
Authors: Kannampallil Thomas , Jones Steve , Abraham Joanna 
Published: July 2019

ObjectiveHandoffs are often framed as the co-construction of a shared understanding relying on narrative storytelling. We investigated how narratives are constructed and used during resident and nurse handoff conversations.MethodWe audio-recorded resident (n=149) and nurse (n=126) handoffs in an inpatient medicine unit. Qualitative analysis using grounded theory was conducted to identify and characterise the structure of resident and nursing handoff narratives.ResultsHandoff conversations among both residents and nurses used three types of narratives: narratives on creating clinical imagery, narratives on coordinating care continuity and narratives on integrating contextual aspects of care. Clinical imagery narratives were common during patient introductions: residents used a top-down approach relying on overarching patient clinical situations (eg, ‘a liver patient’), whereas nurses used a bottom-up approach using patient-specific identifying information. Narratives on the coordination of care continuity for residents focused on managing internal and external coordination activities, whereas nurse narratives focused on internal coordination, emphasising their role as an interface between patients and their physicians. Both resident and nurse narratives on the contextual aspects of care had considerable focus on highlighting ‘heads up’ anticipatory information and personal patient information; such information was often not present in patient charts, but was important for ensuring effective care management.DiscussionThe presence of narrative structures highlights the need for new perspectives for the design of handoff tools that allow for both informational and cognitive support and shared awareness among conversational partners during handoff conversations. We discuss the implications of the use of narratives for patient safety and describe specific design considerations for supporting narrative interactions during handoffs.
Association of trough vedolizumab levels with clinical, biological and endoscopic outcomes during maintenance therapy in inflammatory bowel disease
Authors: Plevris Nikolas , Jenkinson Philip W , Chuah Cher S , Lyons Mathew , Merchant Lynne M , Pattenden Rebecca J , Arnott Ian D , Jones Gareth R , Lees Charlie W 
Published: July 2019

ObjectiveTo establish the relationship between trough vedolizumab levels and outcomes during maintenance therapy.DesignCross-sectional service evaluation was performed on patients with inflammatory bowel disease (IBD) receiving maintenance vedolizumab therapy (minimum of 12 weeks following induction). Prior to infusion, data on clinical activity (Harvey-Bradshaw Index or partial Mayo score), trough C-reactive protein (CRP)/vedolizumab levels and faecal calprotectin were collected. Endoscopic data (±8 weeks from vedolizumab level testing) were obtained by review of medical records. Vedolizumab levels were processed using the Immundiagnostik monitor ELISA.SettingThe Edinburgh IBD Unit, Western General Hospital (tertiary IBD referral centre).PatientsSeventy-three patients (30 ulcerative colitis and 43 Crohn’s disease) were identified who fulfilled inclusion criteria and had vedolizumab levels matched with clinical activity scores, CRP and faecal calprotectin. Of these, 40 patients also had matched endoscopic data.Main outcome measuresThe association of trough vedolizumab levels with clinical remission (Harvey-Bradshaw Index <5 or partial Mayo <2), biologic remission (faecal calprotectin <250 µg/g+CRP <5 mg/L) and endoscopic remission (Mayo score 0/no inflammation and ulceration on colonoscopy).ResultsThe median trough vedolizumab levels were similar between patients in and not in clinical remission (10.6 vs 9.9 µg/mL, p=0.54); biologic remission (10.6 vs 9.8 µg/mL, p=0.35) and endoscopic remission (8.1 vs 10.2 µg/mL, p=0.21). Quartile analysis revealed no significant increase in the proportion of patients in clinical remission, biologic remission or endoscopic remission with increasing trough vedolizumab levels (p<0.05).ConclusionsIn this cohort, trough vedolizumab levels were not associated with clinical, biological or endoscopic outcomes during maintenance therapy.
Expression pattern and prognostic significance of myosin light chain 9 (MYL9): a novel biomarker in glioblastoma
Authors: Kruthika Banavathy S , Sugur Harsha , Nandaki Kanuri , Arimappamagan Arivazhagan , Paturu Kondaiah , Santosh Vani 
Published: July 2019

AimsTumour recurrence is inevitable in glioblastoma (GBM) and mostly noted in the peritumoural brain zone (PT). In our previous microarray-based study, we identified Myosin Light Chain 9 (MYL9) as a highly expressed gene in the PT of GBM. Therefore, we aimed to study the expression pattern and clinical significance of MYL9 in GBM.MethodsPatient samples included three retrospective cohorts: 25 GBM cases with differential biopsies of tumour core and PT, 62 retrospective cases of newly diagnosed GBM with survival information and 20 paired samples (newly diagnosed and recurrent GBM). All tumour tissues, archived as formalin fixed paraffin embedded blocks were retrieved and immunohistochemistry for MYL9 and IDH1 R132H was performed. MYL9 expression was correlated with patient prognosis in our cohort and in The Cancer Genome Atlas (TCGA) and Rembrandt cohorts. It was further evaluated in the 20 paired samples of GBM.ResultsMYL9 showed a cytoplasmic membranous staining of tumour cells. The staining pattern was variable and patchy within tumours. Higher MYL9 expression was associated with poor overall and progression-free survival in our and in TCGA and Rembrandt cohorts. The expression of MYL9 was higher in IDH1 R132H immunonegative cases.ConclusionsWe show MYL9 as a novel biomarker, variably expressed in GBM. The association of high MYL9 expression with poor prognosis in newly diagnosed GBM patients and increased expression in recurrent GBM is indicative of its role in conferring tumour aggressiveness.
Smoke-free and tobacco-free colleges and universities in the United States
Authors: Blake Kelly D , Klein Amanda L , Walpert Laura , Casey Len , Hallett Cynthia , Douglas Clifford , Sinha Bidisha , Koh Howard K 
Published: July 2019

ObjectiveTo describe the number and proportion of accredited, degree-granting institutions with 100% smoke-free and 100% tobacco-free protections across the USA and by state.MethodsData on postsecondary education institutions from the US Department of Education National Center for Education Statistics Integrated Postsecondary Education Data System 2015, and smoke-free and tobacco-free campus protections from the American Nonsmokers’ Rights Foundation’s Smokefree and Tobacco-Free Colleges and Universities List 2017, were integrated to calculate the number and proportion of: (1) smoke-free and tobacco-free accredited, degree-granting institutions and (2) students and staff protected by campus policies and state laws. Campus protections are given a 100% smoke-free designation if smoking is not allowed on campus anywhere, at any time; 100% tobacco-free designations extend smoke-free protections to include non-combustible products such as smokeless tobacco.Results823 accredited, degree-granting institutions (16.7%) representing 1816 individual campuses, sites and schools have either 100% smoke-free or 100% tobacco-free protections. An estimated 14.9 million college students (26.9%) and 8.9 million faculty and staff (25.4%) are protected by campus policies and state laws. Only three states and two territories have 100% smoke-free or 100% tobacco-free protections in over half of their institutions; four states and six territories have no known 100% smoke-free or 100% tobacco-free campus protections.ConclusionsIn 2017, just 16.7% of accredited, degree-granting institutions in the USA had 100% smoke-free or 100% tobacco-free protections. Despite progress, more efforts can ensure that students and staff benefit from comprehensive 100% smoke-free and 100% tobacco-free protections at US colleges and universities.
Impact of typhoon Matmo (2014) on the distribution of heavy metals in Quanzhou Bay
Authors: Lin Yunpeng , Li Yunhai , Zheng Binxin , Yin Xijie , Wang Liang , He Jia , Shu Fangfang 
Published: July 2019

Abstract
The typhoon process has a significant influence on the distribution of heavy metals in sediments. Based on the heavy metal (V, Cr, Co, Ni, Cu, Zn, Pb, and Mn) contents in surface sediments collected under normal conditions and post-typhoon Matmo in Quanzhou Bay in 2014, the distributions, sources, and impacts of typhoon processes on heavy metals and pollution conditions were studied and discussed. The results showed that the heavy metals can be divided into two categories: Class I metals (Cu, Zn, Pb, and Mn) were mainly distributed in the estuary and significantly increased after the typhoon and Class II metals (V, Cr, Co, and Ni) were distributed in the coastal intertidal zone and estuary and remained unchanged or decreased after the typhoon. The heavy metal assessment showed that heavy metal pollution in Quanzhou Bay was serious and tended to increase after the typhoon. The increased metal supply and enhanced riverine and tida hydrodynamics after the typhoon may be the main factors influencing the variations in heavy metal content and distribution. This study provided a basis for the accurate evaluation and scientific management of heavy metal pollution caused by typhoon processes in Quanzhou Bay.
The erosion—deposition process and associated control mechanisms of the Nanliu River subaqueous delta, Southern China coast
Authors: Lin Zhen-Kun , Wang Ai-Jun , Ye Xiang 
Published: July 2019

Abstract
Understanding the erosion–deposition process of sediments and the associated controlling mechanisms in subaqueous deltas is important for coastal environment protection. In this study, field observations and numerical simulations were performed for the Nanliu River subaqueous delta in Guangxi Province (Southern China) to investigate the sediment dynamic processes at the bottom boundary layer. The results show that the sediment resuspension mainly occurs during periods of spring tides and is mainly controlled by the wave action. When the seabed is free from erosion, suspended sediment settling caused by lateral transport is an important source of maintaining near bed suspended sediment concentration. It was also found that increasing the shear parameter could facilitate the formation of flocs, after which the small flocs tend to merge to large flocs. Finally, by performing a consistency analysis between the seabed erosion and deposition processes obtained from numerical simulation, and the changes of seabed level recorded by the equipment during the field observation, we determined that the local erosion coefficient was 5 × 10-5 kg/m2•s. The one-dimensional simulation is also capable of revealing the general trend at the seabed where it is first subjected to erosion and then deposition, as indicated during the field measurements.
Relationship between bowl-shaped clastic injectites and parent sand depletion: implications for their scale-invariant morphology and composition
Authors: Cobain S. L. , Hodgson D. M. , Peakall J. , Silcock S. Y. 
Published: July 2019

Abstract
Three-dimensional seismic reflection data provide a means to assess the impact of injection on parent sands, and to quantify the character of the resulting injectite networks. The morphology of a series of large injectite structures hosted in the Paleocene Lower Lista Formation were mapped using broadband 3D seismic data from the North Sea to investigate their relationship with parent sands. Fourteen bowl-shaped structures were identified within the Lista Formation in the study area (60–85 m in height, and 200–900 m in width). Sand is absent (below resolution) below these large-scale bowls, suggesting that the parent sand is the underlying Maureen Formation and that sand ‘welds’ formed, rather than sand-prone channelized deposits within the Lista Formation.
Identification of injectite networks can be ambiguous, which impacts geological model development. Observations from exhumed systems and core offer high-resolution insights into the complexity of injectite networks. To advance our understanding of this scale gap, we argue for injectites being scale invariant in their shape and grain size. This permits the application of outcrop-scale knowledge to seismic-scale interpretation. The demonstrable depletion of parent sands, and their scale invariance, can be applied to basin-fills worldwide to reduce uncertainties of the impact of sand injectites on hydrocarbon reservoirs.
Load-bearing function of the colorectal submucosa and its relevance to visceral nociception elicited by mechanical stretch
Authors: Siri Saeed , Maier Franz , Santos Stephany , Pierce David M. , Feng Bin 
Published: July 2019

Mechanical distension beyond a particular threshold evokes visceral pain from distal colon and rectum (colorectum), and thus biomechanics plays a central role in visceral nociception. In this study we focused on the layered structure of the colorectum through the wall thickness and determined the biomechanical properties of layer-separated colorectal tissue. We harvested the distal 30 mm of mouse colorectum and dissected this tissue into inner and outer composite layers. The inner composite consists of the mucosa and submucosa, whereas the outer composite includes the muscular layers and serosa. We divided each composite axially into three 10-mm-long segments and conducted biaxial mechanical extension tests and opening-angle measurements for each tissue segment. In addition, we quantified the thickness of the rich collagen network in the submucosa by nonlinear imaging via second-harmonic generation (SHG). Our results reveal that the inner composite is slightly stiffer in the axial direction, whereas the outer composite is stiffer circumferentially. The stiffness of the inner composite in the axial direction is about twice that in the circumferential direction, consistent with the orientations of collagen fibers in the submucosa approximately ±30° to the axial direction. Submucosal thickness measured by SHG showed no difference from proximal to distal colorectum under the load-free condition, which likely contributes to the comparable tension stiffness of the inner composite along the colorectum. This, in turn, strongly indicates the submucosa as the load-bearing structure of the colorectum. This further implies nociceptive roles for the colorectal afferent endings in the submucosa, which likely encode tissue-injurious mechanical distension.NEW & NOTEWORTHY Visceral pain from distal colon and rectum (colorectum) is usually elicited from mechanical distension/stretch, rather than from heating, cutting, or pinching, which usually evoke pain from the skin. We conducted layer-separated biomechanical tests on mouse colorectum and identified an unexpected role of submucosa as the load-bearing structure of the colorectum. Outcomes of this study will focus attention on sensory nerve endings in the submucosa that likely encode tissue-injurious distension/stretch to cause visceral pain.
Myofibrillar protein synthesis and muscle hypertrophy individualized responses to systematically changing resistance training variables in trained young men
Authors: Damas Felipe , Angleri Vitor , Phillips Stuart M. , Witard Oliver C. , Ugrinowitsch Carlos , Santanielo Natalia , Soligon Samuel D. , Costa Luiz A. R. , Lixandrão Manoel E. , Conceição Miguel S. , Libardi Cleiton A. 
Published: July 2019

The manipulation of resistance training (RT) variables is used among athletes, recreational exercisers, and compromised populations (e.g., elderly) attempting to potentiate muscle hypertrophy. However, it is unknown whether an individual’s inherent predisposition dictates the RT-induced muscle hypertrophic response. Resistance-trained young [26 (3) y] men ( n = 20) performed 8 wk unilateral RT (2 times/wk), with 1 leg randomly assigned to a standard progressive RT [control (CON)] and the contralateral leg to a variable RT (VAR; modulating exercise load, volume, contraction type, and interset rest interval). The VAR leg completed all 4 RT variations every 2 wk. Bilateral vastus lateralis cross-sectional area (CSA) was measured, pre- and post-RT and acute integrated myofibrillar protein synthesis (MyoPS) rates were assessed at rest and over 48 h following the final RT session. Muscle CSA increase was similar between CON and VAR ( P > 0.05), despite higher total training volume (TTV) in VAR ( P < 0.05). The 0–48-h integrated MyoPS increase postexercise was slightly greater for VAR than CON ( P < 0.05). All participants were considered “responders” to RT, although none benefited to a greater extent from a specific protocol. Between-subjects variability (MyoPS, 3.30%; CSA, 37.8%) was 40-fold greater than the intrasubject (between legs) variability (MyoPS, 0.08%; CSA, 0.9%). The higher TTV and greater MyoPS response in VAR did not translate to a greater muscle hypertrophic response. Manipulating common RT variables elicited similar muscle hypertrophy than a standard progressive RT program in trained young men. Intrinsic individual factors are key determinants of the MyoPS and change in muscle CSA compared with extrinsic manipulation of common RT variables. NEW & NOTEWORTHY Systematically manipulating resistance training (RT) variables during RT augments the stimulation of myofibrillar protein synthesis (MyoPS) and training volume but fails to potentiate muscle hypertrophy compared with a standard progressive RT. Any modest further MyoPS increase and higher training volumes do not reflect in a greater hypertrophic response. Between-subject variability was 40-fold greater than the variability promoted by extrinsic manipulation of RT variables, indicating that individual intrinsic factors are stronger determinants of the hypertrophic response.