bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cognitive improvement via cortical cannabinoid receptors and choline‐containing lipids
Authors: Moreno‐Rodríguez Marta , Martínez‐Gardeazabal Jonatan , Bengoetxea de Tena Iker , Llorente‐Ovejero Alberto , Lombardero Laura , González de San Román Estibaliz , Giménez‐Llort Lydia , Manuel Iván , Rodríguez‐Puertas Rafael 
Published: November 2024

Background and PurposeRecent research linking choline‐containing lipids to degeneration of basal forebrain cholinergic neurons in neuropathological states illustrates the challenge of balancing lipid integrity with optimal acetylcholine levels, essential for memory preservation. The endocannabinoid system influences learning and memory processes regulated by cholinergic neurotransmission. Therefore, we hypothesised that activation of the endocannabinoid system may confer neuroprotection against cholinergic degeneration.Experimental ApproachWe examined the neuroprotective potential of sub‐chronic treatments with the cannabinoid agonist WIN55,212‐2, using ex vivo organotypic tissue cultures including nucleus basalis magnocellularis and cortex and in vivo rat models of specific cholinergic damage induced by 192IgG‐saporin. Levels of lipids, choline and acetylcholine were measured with histochemical and immunofluorescence assays, along with [35S]GTPγS autoradiography of cannabinoid and muscarinic GPCRs and MALDI‐mass spectrometry imaging analysis. Learning and memory were assessed by the Barnes maze and the novel object recognition test in rats and in the 3xTg‐AD mouse model.Key ResultsDegeneration, induced by 192IgG‐saporin, of baso‐cortical cholinergic pathways resulted in memory deficits and decreased cortical levels of lysophosphatidylcholines (LPC). WIN55,212‐2 restored cortical cholinergic transmission and LPC levels via activation of cannabinoid receptors. This activation altered cortical lipid homeostasis mainly by reducing sphingomyelins in lesioned animals. These modifications were crucial for memory recovery.Conclusion and ImplicationsWe hypothesise that WIN55,212‐2 facilitates an alternative choline source by breaking down sphingomyelins, leading to elevated cortical acetylcholine levels and LPCs. These results imply that altering choline‐containing lipids via activation of cannabinoid receptors presents a promising therapeutic approach for dementia linked to cholinergic dysfunction.
Zymogen granule protein 16B (ZG16B) is a druggable epigenetic target to modulate the mammary extracellular matrix
Authors: Lengyel Máté , Molnár Ádám , Nagy Tamás , Jdeed Sham , Garai Ildikó , Horváth Zsolt , Uray Iván P. 
Published: November 2024

AbstractHigh tissue density of the mammary gland is considered a pro‐tumorigenic factor, hence suppressing the stimuli that induce matrix buildup carries the potential for cancer interception. We found that in non‐malignant mammary epithelial cells the combination of the chemopreventive agents bexarotene (Bex) and carvedilol (Carv) suppresses the zymogen granule protein 16B (ZG16B, PAUF) through an interaction of ARID1A with a proximal enhancer. Bex + Carv also reduced ZG16B levels in vivo in normal breast tissue and MDA‐MB231 tumor xenografts. The relevance of ZG16B is underscored by ongoing clinical trials targeting ZG16B in pancreatic cancers, but its role in breast cancer development is unclear. In immortalized mammary epithelial cells, secreted recombinant ZG16B stimulated mitogenic kinase phosphorylation, detachment and mesenchymal characteristics, and promoted proliferation, motility and clonogenic growth. Highly concerted induction of specific laminin, collagen and integrin isoforms indicated a shift in matrix properties toward increased density and cell‐matrix interactions. Exogenous ZG16B alone blocked Bex + Carv‐mediated control of cell growth and migration, and antagonized Bex + Carv‐induced gene programs regulating cell adhesion and migration. In breast cancer cells ZG16B induced colony formation and anchorage‐independent growth, and stimulated migration in a PI3K/Akt‐dependent manner. In contrast, Bex + Carv inhibited colony formation, reduced Ki67 levels, ZG16B expression and glucose uptake in MDA‐MB231 xenografts. These data establish ZG16B as a druggable pro‐tumorigenic target in breast cell transformation and suggest a key role of the matrisome network in rexinoid‐dependent antitumor activity.
Mitotic Arrest Deficient 2 Like 1 Contributes to Colorectal Cancer Cell Migration, Invasion, and Oxaliplatin Resistance Through the Wnt/β‐Catenin Pathway
Authors: Ding Xiang , Zhou Yonggui , He Linfang , Kang Hubin , Wen Youwu , Xu Jia , Zhu Congbo , Luo Libing , Zeng Qingjun 
Published: November 2024

ABSTRACTColorectal cancer (CRC) is a highly prevalent malignancy, requiring chemotherapy for advanced stages of the disease. Previously, we found that mitotic arrest deficient 2 like 1 (MAD2L1) was upregulated and facilitated malignant proliferation in CRC. However, the association between MAD2L1 expression and tumor progression, as well as chemotherapy resistance in CRC, remains unclear. The progression capacities of CRC cells were assessed using transwell and wound healing assays, and the resistance to cisplatin in oxaliplatin‐resistant CRC cells was assessed using CCK‐8 assay and flow cytometry. Relevant protein levels of epithelial‐to‐mesenchymal transition (EMT) and Wnt/β‐catenin pathway were analyzed using western blotting. Revealing the impact of MAD2L1 on metastasis and drug resistance in CRC through inhibition of the Wnt/β‐catenin pathway. Knockdown of MAD2L1 attenuated the malignant progression of CRC cells, inhibited EMT, and blocked the Wnt/β‐catenin pathway. MAD2L1 was significantly upregulated in oxaliplatin‐resistant CRC cells, accompanied by the activation of the Wnt/β‐catenin pathway. Knockdown of MAD2L1 effectively reversed oxaliplatin resistance, leading to apoptosis and downregulation of the protein expression levels of β‐catenin, P‐glycoprotein (P‐gp), and ABCG2. After the knockdown of MAD2L1, the inhibition of the Wnt/β‐catenin pathway exhibited a synergistic effect, effectively suppressing malignant progression and reversing oxaliplatin resistance in CRC cells. So, knockdown of MAD2L1 suppressed cell malignant progression, equally sensitized resistant CRC cells to oxaliplatin, potentially by blocking the activation of the Wnt/β‐catenin pathway.
University‐based academic programs in addiction studies in the regions of Australia and Aotearoa New Zealand: An overview
Authors: Lososová Amalie , Adams Peter , Miovský Michal 
Published: November 2024

AbstractIntroductionCurrent trends in the addiction field reflect a significant emphasis on the workforce development and education. There are already some data about university‐based addiction studies programs, but not much from Australasia.MethodsThe aim is to provide an overview and describe the academic programs for addiction professionals in Australia and Aotearoa NZ. The research was conducted in 2017 and updated in 2023. Firstly, university websites were searched using pre‐defined keywords, followed by a content analysis of the identified programs. The data were analysed and interpreted by using descriptive statistics.ResultsWe found 21 universities in Australia (13) and Aotearoa NZ (8) where 46 single programs are provided. There are three bachelor programs, nine masters, and the majority of degrees include (post)graduate certificates and diplomas. No doctorate programs are identified. The taught courses provide comprehensive coverage of the addiction field topics. Twelve programs state clearly that there is clinical practice/internship included. Application to most programs requires completion of a relevant degree and in some cases possible clinical experience.Discussion and ConclusionsIn comparison to educational options in other regions, we observe a trend towards preparing university graduates for the workforce, thereby expanding the range of programs at lower levels. Most programs possibly represent clinically oriented education primarily specialising in addictions, and graduate programs in addictions for professionals with other disciplinary bases. Great emphasis is given to the quality standards of education, and also to relationship between education and labour market. Findings help opening opportunities to collaborate globally.
A New Approach for Analyzing Root Development in Autogenous Tooth Transplants Using Computed Tomography
Authors: Beltrame Ludimila Saiter Assis , Bronzato Juliana Delatorre , Almeida Tereza Jacy da Silva , Intra João Batista Gagno , de Britto Cleber Freitas , Frozoni Marcos 
Published: November 2024

ABSTRACTIntroductionThe aim of this study was to evaluate root development in autotransplanted teeth using cone‐beam computed tomography (CBCT) images.MethodsTwelve premolars with incomplete root formation, which were selected to replace prematurely lost upper central incisors, were analyzed by CBCT on two different occasions. The first CBCT examination (T1) was conducted before tooth autotransplantation. The second CBCT examination (T2) was performed over a follow‐up period of at least 12 months and < 5 years. Three previously calibrated evaluators assessed root development. The positions of the tomographic planes were standardized. The mean root length in sagittal and coronal tomographic sections was used to validate the root length at T1 and T2. Longitudinal root development of the transplanted tooth was determined by calculating the difference in root length between T2 and T1. The intraclass correlation coefficient (ICC), paired t‐test, and Pearson test were applied, with significance set at 5%.ResultsThe mean time elapsed between T1 and T2 was 962 days/2.6 years. The ICC was > 0.75. The measurements obtained at T2 were significantly greater than at T1 (p = 0.001). The mean increase in root length was 2.83 mm. There was no significant correlation (p = 0.413; r = 0.261) between root length increase and the time elapsed between T1 and T2.ConclusionPremolar teeth with incompletely formed roots transplanted to the upper central incisor region showed continued root development during postoperative follow‐up.
Daratumumab in the Management of Red Cell Aplasia Following Allogeneic Hematopoietic Stem Cell Transplantation
Authors: Desai Nihar , Viswabandya Auro , Kim Dennis Dong Hwan , Lipton Jeffrey H. , Mattsson Jonas , Law Arjun Datt 
Published: November 2024

ABSTRACTPure red cell aplasia (PRCA) is a rare but significant complication following major ABO‐incompatible allogeneic hematopoietic stem cell transplantation (HSCT). The persistence of recipient B lymphocytes producing anti‐donor isohemagglutinins leads to reticulocytopenia and anemia, often resulting in transfusion dependence. Current treatment options for post‐HSCT PRCA are limited and frequently yield suboptimal responses, complicating patient management. Herein, we report three cases of post‐HSCT PRCA successfully managed with daratumumab, a monoclonal antibody targeting CD38‐expressing plasma cells. All patients demonstrated rapid reticulocyte recovery and transfusion independence after daratumumab treatment, despite prior treatment failures. These findings suggest that daratumumab may provide a more effective therapeutic approach, with a favorable safety profile compared to traditional therapies. Given its demonstrated efficacy and safety, daratumumab warrants consideration as a first‐line treatment for post‐HSCT PRCA, potentially improving patient quality of life and reducing transfusion‐related complications. Further studies should explore optimal dosing and long‐term outcomes.
Latent cognitive phenotypes in juvenile myoclonic epilepsy: Clinical, sociodemographic, and neuroimaging associations
Authors: Struck Aaron F. , Garcia‐Ramos Camille , Prabhakaran Vivek , Nair Veena , Adluru Nagesh , Adluru Anusha , Almane Dace , Jones Jana E. , Hermann Bruce P. 
Published: November 2024

AbstractObjectiveApplication of cluster analytic procedures has advanced understanding of the cognitive heterogeneity inherent in diverse epilepsy syndromes and the associated clinical and neuroimaging features. Application of this unsupervised machine learning approach to the neuropsychological performance of persons with juvenile myoclonic epilepsy (JME) has yet to be attempted, which is the intent of this investigation.MethodsA total of 77 JME participants, 19 unaffected siblings, and 44 unrelated controls, 12 to 25 years of age, were administered a comprehensive neuropsychological battery (intelligence, language, memory, executive function, and processing speed), which was subjected to factor analysis followed by K‐means clustering of the resultant factor scores. Identified cognitive phenotypes were characterized and related to clinical, family, sociodemographic, and cortical and subcortical imaging features.ResultsFactor analysis revealed three underlying cognitive dimensions (general ability, speed/response inhibition, and learning/memory), with JME participants performing worse than unrelated controls across all factor scores, and unaffected siblings performing worse than unrelated controls on the general mental ability and learning/memory factors, with no JME vs sibling differences. K‐means clustering of the factor scores revealed three latent groups including above average (31.4% of participants), average (52.1%), and abnormal performance (16.4%). Participant groups differed in their distributions across the latent groups (p < 0.001), with 23% JME, 22% siblings, and 2% unrelated controls in the abnormal performance group; and 18% JME, 21% siblings, and 59% unrelated controls in the above average group. Clinical epilepsy variables were unassociated with cluster membership, whereas family factors (lower parental education) and abnormally increased thickness and/or volume in the frontal, parietal, and temporal‐occipital regions were associated with the abnormal cognition group.SignificanceDistinct cognitive phenotypes characterize the spectrum of neuropsychological performance of patients with JME for which there is familial (sibling) aggregation. Phenotypic membership was associated with parental (education) and imaging characteristics (increased cortical thickness and volume) but not basic clinical seizure features.
Adaptive Significance of Long Mating With Repeated Intromissions in Zygogramma bicolorata
Authors: Pal Rabi Sankar , Bhowmick Anirban , Naik Kunmun , Nandy Bodhisatta 
Published: November 2024

ABSTRACTLong matings are abundant in insects despite the range of the costs involved. The causes and consequences of the evolution of long mating remain an interesting problem for behavioural ecologists. We studied extraordinarily long mating that involves repeated intromissions interspersed with latent periods in the Parthenium beetle (Zygogramma bicolorata). We conducted a series of interrupted mating assays to examine the fitness consequences of different components of this curious mating behaviour. We tested multiple adaptive hypotheses concerning male fertility and competitive ability. We found that sperm transfer and fertility did not exhibit a linear increase with the number of intromissions. There was also no evidence of nutrient transfer by the males. Interestingly, our results showed that both sexes suffered a significant cost of long mating. Further, female remating behaviour was found to be modulated by the length of the previous mating. Additionally, males were observed performing a curious leg rubbing behaviour during the inter‐intromission latent period, putatively serving as a copulatory courtship function that reduced female resistance to the continuance of mating. Therefore, we show that while the long mating may still serve a mate‐guarding role, there are additional fitness effects of such behaviour that need careful consideration. Our study provides insights into the adaptive significance of long mating and its fitness consequences.
Exploring the multifaceted impact of breast cancer on the body image
Authors: Davis Amelia , Acevedo Genevieve , Crowley‐Miller Alyssa , Tran Angel , Shah Malay , Langlais Mickey , Sturdivant Rodney , Elkins Gary , Yoo Jeong‐Ju 
Published: November 2024

AbstractThe goal of the study is to describe variables contributing to body image concerns among breast cancer survivors to foster positive body image. Participants for this qualitative study were recruited through convenience sampling (N = 10). The participants' ages ranged from 48 to 80, with an average of 64 years old. Data were analyzed using reflexive thematic analysis. Four themes concerned with body image were age at diagnosis, intimate partnership, preparedness for physical changes, and mental fluidity. Results from this study were used to develop strategies that can be adopted to improve the body image of cancer patients and survivors.
The Feasibility of a Claw‐Only Fishery for the Red Rock Crab, Cancer productus
Authors: McGaw Iain J. , Curtis Daniel L. , Van Leeuwen Travis E. , Schneider Eric V. C. 
Published: November 2024

ABSTRACTThe red rock crab, Cancer productus, is primarily harvested by a recreational fishery, with a small commercial harvest in California. Although relatively large, meat yields are low because the legs are small, and meat is largely confined to the claws. We investigated the potential for a claw‐only harvest for this species. Although hand removal is used in the stone crab fishery, it caused > 90% post‐harvest mortality in red rock crabs. Inducing autotomy by inserting a spike into the arthrodial membranes or by cutting into the autotomy plane resulted in a high post‐harvest survival. We recommend a minimum harvest size of 35 mm claw height to produce a claw size similar to medium‐sized stone crabs and standard‐sized brown and Jonah crabs and to ensure that severed claws are unlikely to have come from female crabs, which is important for regions that mandate a male‐only harvest.
Recreational Fishing in the Neotropical Region: A Systematic Review
Authors: da Silva Ladislau Daniel , de Souza Pauliana Leão , Gubiani Éder André 
Published: November 2024

ABSTRACTWe reviewed the scientific literature to assess the current status of recreational fishing in the Neotropical region. In total, 122 publications met our criteria and are included here. Since 1990, the number of studies published on recreational fishing in the region significantly increased. Recreational fishing was evaluated in 13 countries and two territories. Most studies were in marine environments. In total, 372 fish species captured through recreational Neotropical fishing were registered. In South American countries, the presence of nonnative species was significant. Studies were concentrated in a few countries or were not published in scientific articles, conference papers, or book chapters, and some aquatic environments, such as coastal areas, bays, coastal lagoons, and reservoirs, were not studied. In addition, there are few lines of investigation into the practice of this activity in the region, and new investigations are necessary, especially with respect to environmental problems.
A Decade of Monitoring Reveals a Dynamic Fish Assemblage on a Substantial Artificial Reef in the Texas Gulf of Mexico
Authors: Hicks David , Getz Ethan , Kline Richard , Cintra Buenrostro Carlos 
Published: November 2024

ABSTRACTFish communities on artificial reefs have rarely been monitored over long periods to evaluate success of reef deployment. Here, we used roving diver surveys to assess changes in fish assemblages on a large, reefed vessel during 2008–2017. Multivariate analyses revealed a dynamic community that stabilized after 5 years. Species richness increased and species dominance decreased during 2008–2017. The fish community shifted toward reef‐associated species such as hogfish and pufferfish. Species composition shifted, but trophic structure was relatively stable, which suggested that functional groups may not reflect larger community shifts. Our results indicate that fish communities on artificial reefs are temporally dynamic and that long‐term monitoring is needed to understand how fish assemblage structure changes through time.
Map‐Like Redness Development After Eradication Therapy for Helicobacter pylori Infection: Prospective Multicenter Observational Study
Authors: Matsumoto Sho , Sugimoto Mitsushige , Terai Tomohiro , Maruyama Yasuhiko , Sahara Shu , Kanaoka Shigeru , Yoshizawa Yashiro , Unno Shuhei , Murata Masaki , Uotani Takahiro , Sugiyama Tomohiro , Nakajima Shigemi , Hayafuji Kiyoyuki , Haruma Ken , Kamada Tomoari , Fukuzawa Masakatsu , Kawai Takashi , Itoi Takao 
Published: November 2024

ABSTRACTBackgroundMap‐like redness, pathological intestinal metaplasia, is observed in one‐fourth to one‐third of patients 1 year after Helicobacter pylori eradication therapy, mainly in the corpus, and is a newly identified endoscopic risk factor for gastric cancer development after eradication. However, it is unclear whether intestinal metaplasia is present before eradication at the site where the map‐like redness appears. We aimed to identify endoscopic findings that predict the occurrence of map‐like redness before H. pylori eradication.Materials and MethodsAs a prospective multicenter trial, the characteristics of patients in whom map‐like redness developed after eradication, and the association between the endoscopic severity of gastritis and the development of map‐like redness in patients who underwent endoscopy before and 1‐year after eradication were investigated.ResultsThe rate of map‐like redness in all 93 patients 1‐year postsuccessful eradication was 30.1% (95% confidence interval [CI]: 21.0–40.5). All patients with map‐like redness were endoscopically observed to have intestinal metaplasia before eradication, in the site that subsequently developed map‐like redness. Patients who developed map‐like redness were older, had more severe intestinal metaplasia and nodularity and a higher total score on the Kyoto Classification of Gastritis both before and after eradication than patients who did not. On multivariate analysis, map‐like redness was found to be associated with posttreatment intestinal metaplasia (odds ratio: 8.144; 95% CI: 2.811–23.592).ConclusionsIn all patients who developed map‐like redness after eradication, endoscopic intestinal metaplasia was observed at the site developed map‐like redness before eradication therapy. Map‐like redness was especially observed in patients with more severe intestinal metaplasia at 1‐year after eradication. Such patients require increased attention at surveillance endoscopy, owing to generally having a higher risk of gastric cancer development.Trial RegistrationUniversity Hospital Medical Information Network: UMIN000044707