bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum exosomal microRNA let‐7i‐3p as candidate diagnostic biomarker for Kawasaki disease patients with coronary artery aneurysm
Authors: Wang Yan‐Fei , Lian Xin‐Lei , Zhong Jia‐Yong , Su Shi‐Xin , Xu Yu‐Fen , Xie Xiao‐Fei , Wang Zhou‐Ping , Li Wei , Zhang Li , Che Di , Yu Li , Huang Ping , Jia Hong‐Ling , Gu Xiao‐Qiong 
Published: February 2019

ABSTRACTKawasaki disease (KD) is a systemic vasculitis syndrome that leads to coronary artery aneurysm (CAA). While echocardiography is the most important imaging modality for coronary artery assessment, a specific diagnostic biomarker complementary for CAA has not been reported. We aimed to analyze the profiles of exosomal miRNAs extracted from the serum of KD patients and controls to identify candidate biomarkers for CAA. Serum samples from 39 healthy children, 42 CAA patients, 38 coronary artery dilatation (CAD) patients and 45 virus‐infected patients including 24 EBV patients and 21 ADV patients were randomly selected. Next generation sequencing was used to analyze serum exosomal miRNA to detect differentially expressed miRNAs. Biomarker candidates were validated by qRT‐PCR. One hundred (and) ninety‐six differentially expressed miRNAs (DEMs) were detected in CAA patients and healthy children. There were 70 DEMs and 140 DEMs in CAA patients versus CAD patients, and in CAA patients versus virus‐infected patients, respectively. We selected the three most upregulated (let‐7i‐3p, miR‐17‐3p, and miR‐210‐5p) and the three most downregulated miRNAs (miR‐6743‐5p, miR‐1246, and miR‐6834‐5p) in the DEMs, which were expressed differentially in CAA patients versus healthy children, and in CAA patients versus virus‐infected patients, not in virus‐infected patients versus healthy children, as biomarker candidates. Excluded DEMs of CAD and virus‐infected patients, let‐7i‐3p was detected by sequence data analysis as a biomarker candidate for CAA patients, and then validated by qRT‐PCR in a larger set of clinical samples. As a biomarker candidate, let‐7i‐3p provides an additional means of diagnosing CAA patients. Additionally, miRNA biomarkers complement ultrasonic imaging, allowing for greater diagnostic precision. © 2019 IUBMB Life, 2019
T cell engineered with a novel nanobody‐based chimeric antigen receptor against VEGFR2 as a candidate for tumor immunotherapy
Authors: Hajari Taheri Fatemeh , Hassani Mahmoud , Sharifzadeh Zahra , Behdani Mehdi , Arashkia Arash , Abolhassani Mohsen 
Published: February 2019

AbstractSolid tumors that are responsible for more than 85% of cancer death cases need angiogenesis for their growth and metastasis. Among antiangiogenic therapies, targeting the vascular endothelial growth factor receptor 2 (VEGFR2) that is over‐expressed on tumor vasculatures has been a promising strategy. In this study, we developed a second generation nanobody (VHH)‐based CAR T cell targeting VEGFR2‐expressing tumor cells. The CAR T cell was developed by linking the anti‐VEGFR2 VHH to a spacer, and signaling domains of CD28 and CD3 ζ. The T cells were activated with anti‐CD3 plus rIL‐2 and electroporated with a plasmid encoding the CAR construct. The expression of activation markers, CD69 and CD25, on CAR T cells upon coculturing with VEGFR2‐expressing cells were 41% and 48%, and the IL‐2 and IFN‐γ production were 470 pg/mL and 360 pg/mL, respectively. The expression of degranulation marker, CD107a, was 30% and the cytotoxic activity of the CAR T cells reached to more than 30% with E:T ratio of 9:1. The anti‐VEGFR2 CAR but not mock T cells mediated specific lysis of 293‐KDR cells expressing human VEGFR2 and might be considered as a candidate for adoptive T‐cell immunotherapy of solid tumors. © 2019 IUBMB Life, 71(9):1259–1267, 2019
Creation of a unique architectural structure of bioactive glass sub‐micron particles incorporated in a polycaprolactone/gelatin fibrous mat; characterization, bioactivity, and cellular evaluations
Authors: Fanaee Sajjad , Labbaf Sheyda , Enayati Mohammad Hossein , Baharlou Houreh Arezou , Esfahani Mohammad‐Hossein Nasr 
Published: February 2019

AbstractIn this study, submicron, monodispersed, spherical bioactive glass (BG) particles with a mean diameter of 720 ± 80 nm were produced through sol–gel process. The prepared BG particles were successfully incorporated into 70/30 (W/W) ratio of polycaprolactone/gelatin (PCL/GT) nanofibrous mats (250 ± 60 nm) through electrospinning to obtain a unique architectural structure for the first time. To enhance biodegradation and stability of the scaffolds, crosslinking using gluteraldehyde was applied. The structure, wettability, hydroxyapatite (HA) formation, cell adhesion, cell viability and osteogenic potential of the fibrous mats were evaluated. A continuous, uniform and hydrophilic structure of PCL/GT/BG was obtained. The fibers were found to be bioactive as they formed HA on their surface after immersion in simulated body fluid. The unique structure significantly reduced HA formation time to 5 days. For in vitro investigations, human dental pulp stem cells (hDPSCs) were cultured on PCL/GT and PCL/GT/BG fibrous mats. Results demonstrated good cell attachment after 4 and 24 h with no significant levels of cytotoxicity during 10 days of culture. Alizarin red staining was applied to quantitatively analyze the potential of PCL/GT/BG in osteogenic differentiation of hDPSCs. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.
Substance P inhibits high urea‐induced apoptosis through the AKT/GSK‐3β pathway in human corneal epithelial cells
Authors: Wang Peng , Me Rao , Yuan Ying , Yu Yunjie , Li Min , Ke Bilian 
Published: February 2019

AbstractTo investigate the effect of substance P (SP) on human corneal epithelial cells (HCECs) that have been stressed by a high urea environment and to determine the relationship between SP and the protein kinase B (AKT)/glycogen synthase kinase‐3β (GSK‐3β) signaling pathway. An in vitro model of chronic renal failure (CRF)‐related dry eye was used to study HCECs that were treated with high urea concentrations. Cell proliferation was assayed using a cell counting kit‐8 test. Besides, cell apoptosis was evaluated by flow cytometry. Furthermore, the effects of SP and the AKT inhibitor perifosine on the urea‐treated HCECs were examined using immunofluorescence, quantitative real time polymerase chain reaction (qRT‐PCR), and Western blot analysis. SP markedly reduced the number of apoptotic HCECs and decreased the cleaved caspase‐3 expression levels while contributing to increased cellular proliferation (P < 0.05). The Western blot analysis and qRT‐PCR experiments revealed that SP significantly increased the expression of p‐AKT and p‐GSK‐3β (P < 0.05); additionally, these increases were attenuated after the perifosine inhibition of the AKT signaling pathway (P < 0.05). These in vitro experiments demonstrated that SP may protect against the apoptotic damage of HCECs caused by the high urea condition. The underlying mechanism may be related to the activation of the AKT/GSK‐3β signaling pathway.
How useful is bone marrow study as an initial investigative tool without lymph node biopsy in malignant lymphoma?: Eleven years of experience at a single institution
Authors: Kwoun Woo‐Jae , Ahn Jeong‐Yeal , Park Pil‐Whan , Seo Yiel‐Hea , Kim Kyung‐Hee , Seo Ja Young , Lee Hwan Tae , Yoo Kuai‐Han 
Published: February 2019

BackgroundBone marrow (BM) study plays an important role as initial investigation specimen of lymphoma as well as staging lymphoma. This study aimed to investigate the utility of BM studies for classification of lymphoma and evaluate features of BM involvement by lymphoma over a period of 11 years.MethodsA total of 1162 cases of BM studies for lymphoma evaluation were reviewed for the incidence of lymphoma subtypes, the percentage of marrow involvement, the pattern of involvement and discordance with histopathologic diagnoses of lymph nodes and other tissues.ResultsA total of 255 of 1162 cases underwent BM study without pathologic information, and 108 cases show lymphoma involvement. Lymph node biopsy underwent in 66 cases, and 10 cases show discordant result between BM and lymph node biopsy. Seven discordant cases were due to insufficient further studies. Lymphoma was diagnosed only by BM study in 38 cases. Abnormal lymphocytes were found in BM aspiration in 34 cases. Also, abnormal clonal lymphocytes were detected by flow cytometry in 26 cases. Four cases showed disease‐related chromosomal abnormalities. FISH analysis detected abnormal findings in two cases, however, discordant with other additional studies.ConclusionsDiscrepancies between the BM study and lymph node biopsy were due to insufficient further study and discordance of immunohistochemical stain result. BM study can be utilized as initial diagnosis of lymphoma by the combination of morphological feature, involvement pattern, and additional tests such as flow cytometry, chromosomal analysis, and FISH analysis. Thus, BM study with further analysis is an essential choice when lymph node biopsies are unavailable.
PrEP uptake preferences among men who have sex with men in China: results from a National Internet Survey
Authors: Han Jing , Bouey Jennifer ZH , Wang Liming , Mi Guodong , Chen Zihuang , He Ying , Viviani Tara , Zhang Fujie 
Published: February 2019

AbstractIntroductionHIV incidence among men who have sex with men (MSM) is high in China. Pre‐exposure prophylaxis (PrEP) is a promising mean to prevent HIV transmission but it is not widely available in China. We conducted a large Internet‐based online survey to assess the willingness of Chinese MSM to take PrEP and associated factors to their uptake preferences.MethodsBetween 19 January and 6 February, 2017, 4581 MSM aged over 15 years were recruited via a social networking app to take an online PrEP survey. HIV status at the time of the survey being conducted was not one of recruitment criteria. Participants were asked if they had heard of PrEP, if they had concerns about PrEP, and if they would be ready to uptake PrEP should it be provided. When asked if participants were willing to take PrEP, they were asked to select from the following responses: “definitely not,” “probably not,” “not sure,” “probably yes,” and “definitely yes.” In the final analysis, we grouped these five‐level Likert scale responses into three‐level responses as “definitely yes,” “probably yes,” and “no (definitely not/probably not/not sure).” Descriptive analysis and multinomial logistic regressions were conducted to assess the associations of PrEP adoption readiness and uptake concerns with HIV risk behaviours and demographic characteristics.ResultsMSM from 33 geographical regions of China participated in the survey. The majority were younger than 25 (65.2%) and had attended college (68.6%). HIV prevalence was high (6.8%) and 43.3% reported a history of unprotected anal sex. Only 22.4% of participants had heard of PrEP. When asked if they would uptake PrEP, 26.0% said “definitely yes,” 49.6% were “probably yes,” and 24.4% said “no.” PrEP adoption readiness was associated with having previously heard of PrEP and expressing concerns about accessibility and cost. Worries about side effects, low perceived HIV risk, preference for condoms, and never having received HIV testing were negatively associated with PrEP uptake willingness.ConclusionYoung and well‐educated Chinese MSM reported a low willingness to uptake PrEP despite being high‐risk for HIV. Effective education, especially through online mediums, will be critical to optimize this group's PrEP uptake.
Paraspinal Muscle DTI Metrics Predict Muscle Strength
Authors: Klupp Elisabeth , Cervantes Barbara , Schlaeger Sarah , Inhuber Stephanie , Kreuzpointer Florian , Schwirtz Ansgar , Rohrmeier Alexander , Dieckmeyer Michael , Hedderich Dennis M. , Diefenbach Maximilian N. , Freitag Friedemann , Rummeny Ernst J. , Zimmer Claus , Kirschke Jan S. , Karampinos Dimitrios C. , Baum Thomas 
Published: February 2019

BackgroundThe paraspinal muscles play an important role in the onset and progression of lower back pain. It would be of clinical interest to identify imaging biomarkers of the paraspinal musculature that are related to muscle function and strength. Diffusion tensor imaging (DTI) enables the microstructural examination of muscle tissue and its pathological changes.PurposeTo investigate associations of DTI parameters of the lumbar paraspinal muscles with isometric strength measurements in healthy volunteers.Study TypeProspective.SubjectsTwenty‐one healthy subjects (12 male, 9 female; age = 30.1 ± 5.6 years; body mass index [BMI] = 27.5 ± 2.6 kg/m2) were recruited.Field Strength/Sequence3 T/single‐shot echo planar imaging (ss‐EPI) DTI in 24 directions; six‐echo 3D spoiled gradient echo sequence for chemical shift encoding‐based water–fat separation.AssessmentParaspinal muscles at the lumbar spine were examined. Erector spinae muscles were segmented bilaterally; cross‐sectional area (CSA), proton density fat fraction (PDFF), and DTI parameters were calculated. Muscle flexion and extension maximum isometric torque values [Nm] at the back were measured with an isokinetic dynamometer and the ratio of extension to flexion strength (E/F) calculated.Statistical TestsPearson correlation coefficients; multivariate regression models.ResultsSignificant positive correlations were found between the ratio of extension to flexion (E/F) strength and mean diffusivity (MD) (P = 0.019), RD (P = 0.02) and the eigenvalues (λ1: P = 0.026, λ2: P = 0.033, λ3: P = 0.014). In multivariate regression models λ3 of the erector spinae muscle λ3 and gender remained statistically significant predictors of E/F (R2adj = 0.42, P = 0.003).Data ConclusionDTI allowed the identification of muscle microstructure differences related to back muscle function that were not reflected by CSA and PDFF. DTI may potentially track subtle changes of back muscle tissue composition.Level of Evidence: 3Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;50:816–823.
Molecular Characterization of Microvesicular and Macrovesicular Steatosis Shows Widespread Differences in Metabolic Pathways
Authors: Kristiansen Maria N. B. , Veidal Sanne S. , Christoffersen Christina , Jelsing Jacob , Rigbolt Kristoffer T. G. 
Published: February 2019

AbstractSimple steatosis is the hallmark of nonalcoholic fatty liver disease, with lipid accumulating as either microvesicular or macrovesicular lipid droplets within hepatocytes. The present study used a combination of laser capture microdissection and RNAseq to characterize murine gene expression in nonsteatotic, microsteatotic, and macrosteatotic compartments collected from the same liver. The data indicate that microvesicular steatosis is intermediate to macrovesicular steatosis, showing a widespread and pronounced metabolic gene regulation of lipid export, gluconeogenesis, and de novo lipogenesis. Key enzymes, such as fatty acid synthase and fructose 1,6‐bisphosphatase as well as apolipoprotein C‐III, were identified to show clear expression differences between the compartments. Furthermore, increased expression of lipid particle formation genes provided a molecular description of the fusion of microsteatotic lipid compartments to produce macrosteatotic cells with a single enlarged lipid droplet.
Technical Note: T1 and T2 and complex permittivities of mineral oil, silicone oil, and glycerol at 0.35, 1.5, and 3 T
Authors: Gach H. Michael 
Published: February 2019

PurposeTo identify an inexpensive, low‐dielectric liquid for large magnetic resonance imaging (MRI) phantoms that can be used at multiple magnetic field strengths.MethodsThe T1 and T2 of four candidate phantom liquids (pure mineral oil, food‐grade white mineral oil, silicone oil, and glycerol) with low dielectric constants were measured at three field strengths (0.35, 1.5, and 3 T) and extrapolated for 7 T. The complex permittivities of the liquids were measured for frequencies from 13 to 600 MHz. Proton densities were calculated based on molecular weight, proton number, and density. The results were compared to the American College of Radiology (ACR) large MRI phantom electrolyte liquid (10 mM NiCl2 and 75 mM NaCl in water) and deionized water. The liquids were evaluated based on the NEMA standards (T1 < 1200 ms, T2 > 50 ms, proton density within 20% of water, and produces minimal dielectric artifacts). The radiofrequency (RF) wavelengths were computed for each liquid at the four field strengths to determine the risk of dielectric artifacts.ResultsThe mineral oils were the only liquids to satisfy all of the NEMA guidelines. Excluding deionized water, silicone oil had the longest T1 and T2, and was the most expensive liquid ($200/L). Glycerol had the shortest T1 and T2, and the highest dielectric (excluding the ACR phantom electrolyte and deionized water). All of the liquids except silicone oil met the NEMA proton density guidelines.ConclusionsFood‐grade white mineral oil is a good candidate for use in a phantom due to its relaxation times, low dielectric, high proton density, and low cost. Glycerol and deionized water are poor choices for phantom liquids due to their relaxation times and high dielectric constants.
Nonneutropenic fever in children with cancer: A scoping review of management and outcome
Authors: Allaway Zoe , Phillips Robert S. , Thursky Karin A. , Haeusler Gabrielle M. 
Published: February 2019

AbstractTo date, very few studies have addressed nonneutropenic fever (NNF) in children with cancer, and there are no consensus guidelines. This scoping review aims to describe the rate of bacteremia, risk factors for infection and management, and outcomes of NNF in this population. Across 15 studies (n = 4106 episodes), the pooled‐average bacteremia rate was 8.2%, and risk factors included tunneled external central venous catheter, clinical instability, and higher temperature. In two studies, antibiotics were successfully withheld in a subset of low‐risk patients. Overall outcomes of NNF appear favorable; however, further research is required to determine its true clinical and economic impact.
Back‐contacted bottom cells with three terminals: Maximizing power extraction from current‐mismatched tandem cells
Authors: Rienäcker Michael , Warren Emily L. , Schnabel Manuel , Schulte‐Huxel Henning , Niepelt Raphael , Brendel Rolf , Stradins Pauls , Tamboli Adele C. , Peibst Robby 
Published: February 2019

AbstractMulti‐junction cells can significantly improve the energy yield of photovoltaic systems over a single‐junction cell. The internal interconnection scheme of the subcells is an important aspect in determining the resulting levelized cost of electricity. For a dual‐junction cell, two approaches are commonly discussed: series‐connected tandem cells with two terminals or independently working subcells in a four‐terminal (4T) tandem device.In this paper, we explore the working principle and the operation modes of a third, rarely discussed option: a three‐terminal (3T) tandem cell using a back‐contacted bottom cell with 3Ts. We use current–voltage measurements of illuminated 3T interdigitated back contact cells and confirm that the front and rear base contacts are at similar quasi‐Fermi level positions, which enables the bottom cell to either efficiently collect surplus carriers, in the case of a current‐limiting or carrier injecting top cell, or inject majority carriers, in the case of a current‐limiting bottom cell. As a result, no current matching is needed. The power output of an idealized 3T bottom cell without resistive effects is independent of the current density applied from the top cell. These characteristics of the 3T bottom cells enable a 3T tandem to operate as efficiently as a 4T tandem, while being compatible with monolithic design and not requiring intermediate grids.We propose a simple equivalent circuit model including additional resistive effects, which describes a real 3T bottom cell and achieves excellent agreement to the experiment. We deduce design guidelines for a 3T bottom cell in different operation regimes.
Monitoring of user‐generated reviews via a sequential reverse joint sentiment‐topic model
Authors: Liang Qiao , Wang Kaibo 
Published: February 2019

AbstractUser‐generated reviews can serve as an efficient tool for evaluating the customer‐perceived quality of online products and services. This article proposes a joint control chart for monitoring the quantitative evolution of document‐level topics and sentiments in online customer reviews. A sequential model is constructed to convert the temporally correlated document collections to topic and sentiment distributions, which are subsequently used to monitor the topics that users are concerned about and the topic‐specific opinions in an ongoing product and service process. Simulation studies on various data scenarios demonstrate the superior performance of the proposed control chart in terms of both detecting shifts and identifying truly out‐of‐control terms.
ZnFe2O4‐Decorated Mesoporous Al2O3 Modified MCM‐41: A Solar‐Light‐Active Photocatalyst for the Effective Removal of Phenol and Cr (VI) from Water
Authors: Das Kundan Kumar , Patnaik Sulagna , Nanda Binita , Pradhan Amaresh Chandra , Parida Kulamani 
Published: February 2019

AbstractA series of promising visible light driven ZnFe2O4 modified Al2O3−MCM‐41 nanocomposites were designed by wet impregnation method by loading different wt % (2, 6, 10) of ZnFe2O4 on the surface of mesoporous Al2O3−MCM‐41. All the prepared photocatalysts were analyzed by using X‐ray diffractometers (XRD), Scanning electron microscope (SEM), N2 sorption, Ultra violet‐Visible Diffuse reflectance spectroscopy (UV‐Vis DRS), X‐ray photoelectron spectroscopy (XPS), Fourier‐transmission infrared spectroscopy (FTIR) and High‐resolution transmission electron microscope (HRTEM) techniques to uncover and confirm the formation of phase, morphology and porous structure of the prepared nanocomposites. In the photo‐Fenton reaction, the ZnFe2O4 modified Al2O3−MCM‐41 nanocomposites exhibit a significant catalytic activity for the photo‐Fenton degradation of phenol (40 min, 99%) via photocatalysis and photocatalytic reduction of Cr (VI) (60 min, 66%) under sunlight with an excellent reusability nature. The high photo‐Fenton activity of the composites is mainly attributed to mesoporosity and high surface area of support Al2O3−MCM‐41, proper light harvestation and increased charge transfer efficiency by active species ZnFe2O4 , and suitable band edge potential for hydroxyl radical generation. The photo‐Fenton activities were further supported by the high photocurrent (0.29 mA/cm2) generated by the nanocomposite ZnFe2O4 modified Al2O3−MCM‐41. These results open up a new route for ZnFe2O4 modified Al2O3−MCM‐41as an efficient photo‐Fenton catalyst for environmental remediation with superior activity and higher stability.
Palladium (II) Catalyzed Arylation and Methylene Oxidation of 2, 7‐Dibromo Fluorenes with Heteroaryl Esters: Synthesis of Mesogenic2‐Heteroaryl and 2, 7‐Diheteroaryl‐9‐fluorenones
Authors: Pratap Gallelli , Narasimhaswamy Tanneru , Shanmugam Ponnusamy 
Published: February 2019

AbstractA facile and unusual palladium catalyzed synthesis of 2‐aryl and 2,7‐diheteroaryl‐9‐fluorenones via a one‐pot CH‐activation‐coupling‐oxidation of 2‐bromo and 2,7‐dibromofluorenes with heteroaryl esters have been achieved. Plausible mechanism for the formation of title compounds invoking palladium mediated direct arylation followed by methylene oxidation is provided. Significantly, among the synthesized compounds, 2,7‐dithiophene‐9‐fluorenones showed enantiotropic smectic mesophases and also optical emission.