bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Fc gamma‐receptor genes polymorphisms with chronic periodontitis and Peri‐Implantitis
Authors: Saremi Leila , Esmaeilzadeh Emran , Ghorashi Tahereh , Sohrabi Maryam , Ekhlasmand Kermani Mehdi , Kadkhodazadeh Mahdi 
Published: March 2019

AbstractThis study was conducted on 87 patients with chronic periodontitis (CP), 50 patients with peri‐implantitis and 90 periodontally healthy individuals referring to the Department of Periodontics for evaluating the association between Fc gamma‐receptor genes polymorphisms with CP and peri‐implantitis. After obtaining consent, venous blood samples (5cc) were obtained from patients and DNA was extracted using Miller's salting‐out method. Polymerase chain reaction (PCR)‐restriction fragment length polymorphism and tetra‐primer amplification refractory mutation system‐PCR methods were used to assess the polymorphisms of FcγRs IIa,
IIIa, and
IIIb genes. Analyzing showed a significant association between specific genotypes with increasing CP and peri‐implantitis risks in codominant and dominant models. For
FcγR IIIa, analyzing revealed a significant association between specific genotypes with increasing CP and peri‐implantitis risks in codominant, dominant, and recessive models. For
FcγR IIIb, we also detected a significant association between specific genotypes with increasing CP and peri‐implantitis risks in codominant, dominant, and recessive models (
P < 0.05). According to the results of this study, the
FCGRIIa (rs1801274),
FCGRIIIa (rs396991), and
FCGRIIIb (rs1050501) polymorphisms were significantly associated with CP and peri‐implantitis and may have a role in the pathogenesis of these diseases.
Elevated expression of ICAM‐1 in synovium is associated with early inflammatory response for cartilage degeneration in type 2 diabetes mellitus
Authors: Gui Tao , Lin Yong‐Kai , Huan Song‐Wei , Li Yu‐Hang , Wang Bao‐Hua , Yang Jie , Gu Rong‐He , Liu Qing , Li Zhen‐Yan , Li Sai‐Mei , Chen Yuan‐Feng , Zhang Huan‐Tian , Zha Zhen‐Gang 
Published: March 2019

AbstractType 2 diabetes mellitus (T2DM) is increasingly being recognized as an independent risk factor for the onset and progression of osteoarthritis (OA). Extensive studies have focused on the contribution of obesity (excessive mechanical stress), comorbidity frequently found in T2DM, to cartilage destruction during OA development. However, a little is known about how diabetes‐related inflammation may affect the local cartilage in a diabetic objective. In the present study, we were able to establish a T2DM rat model using a combination of a low dose of streptozotocin with high‐fat and high‐sugar diet. Although the cartilage integrity was comparable between the control and T2DM groups, the expression of matrix metalloproteinases‐13 (MMP‐13) was significantly upregulated in T2DM, indicating the initiation of an early cascade of cartilage degeneration. In parallel, an obvious alteration of subchondral bone remodeling (inhibition of bone formation) was observed, as evidenced by the reduction of osterix‐expressing positive cells. Moreover, we demonstrated that the expression of intercellular adhesion molecule‐1 (ICAM‐1) in the serum and synovium of T2DM rats was elevated, accompanied by an increase of synovitis score. We also noticed that the number of F4/80‐positive macrophage cells was significantly increased in the T2DM group. Mechanistically, the expression of ICAM‐1 in fibroblast‐like synoviocytes can be triggered by glucose and interleukin‐1β, which are the two important factors within the joint of T2DM. Given that MMP‐13 expression was significantly upregulated in the T2DM cartilage, and that ICAM‐1‐mediated filtration of macrophage was associated with synovitis, we propose that ICAM‐1 is essential for triggering a vicious cycle of inflammation within the joint, which together subsequently drivers the cartilage degradation.
FKBP12: A partner of Snx10 required for vesicular trafficking in osteoclasts
Authors: Battaglino Ricardo A. , Jha Prakash , Sultana Farhath , Liu Weimin , Morse Leslie R. 
Published: March 2019

AbstractOsteoclasts employ highly specialized intracellular trafficking controls for bone resorption and organelle homeostasis. The sorting nexin Snx10 is a (Phosphatidylinositol 3‐phosphate) PI3P‐binding protein, which localizes to osteoclast early endosomes. Osteoclasts from humans and mice lacking functional Snx10 are severely dysfunctional. They show marked impairments in endocytosis, extracellular acidification, ruffled border formation, and bone resorption, suggesting that Snx10 regulates membrane trafficking. To better understand how SNx10 regulates vesicular formation and trafficking in osteoclasts, we set out on a search for Snx10 partners. We performed a yeast two‐hybrid screening and identified FKBP12. FKBP12 is expressed in receptor activator of nuclear factor kB ligand–stimulated RAW264.7 monocytes, coimmunoprecipitates with Snx10, and colocalizes with Snx10 in osteoclasts. We also found that FKBP12, Snx10, and early endosome antigen 1 (EEA1) are present in the same subcellular fractions obtained by centrifugation in sucrose gradients, which confirms localization of FKBP12 to early endosomes. Taken together, these results indicate that Snx10 and FKBP12 are partners and suggest that Snx10 and FKBP12 are involved in the regulation of endosome/lysosome homeostasis via the synthesis. These findings may suggest novel therapeutic approaches to control bone loss by targeting essential steps in osteoclast membrane trafficking.
Cognitive heuristics in borderline personality disorder across treatment: A longitudinal non‐parametric analysis
Authors: Kramer Ueli , Golam Mehdi 
Published: March 2019

AbstractObjectiveThe development of a constructive therapeutic alliance may represent an important feature of interpersonal adaptation in clients with Borderline Personality Disorder (BPD). The present study explores cognitive heuristics as dynamic features of change in relationship with the therapeutic alliance in the treatment of BPD.MethodIn total, N = 60 clients with BPD, are included in the present study. In the context of brief therapy, the therapeutic alliance (WAI) is assessed from the client and the therapist perspectives after each therapy session; cognitive heuristics are assessed three times (CERS). The data analyses are on the basis of non‐parametric clusters (kml3d) linked with the therapeutic alliance.ResultsThe results showed that clusters of cognitive heuristics trajectories are linked with the client's therapeutic alliance (t(55) = 2.30,
p = .03), but they remained unrelated with the evolution of the therapist's alliance.ConclusionsThese results are discussed with regard to the interpersonal adaptiveness of cognitive heuristics in the context of BPD undergoing treatment.
Role of SCFAs in gut microbiome and glycolysis for colorectal cancer therapy
Authors: Wang Gang , Yu Yang , Wang Yu‐Zhu , Wang Jun‐Jie , Guan Rui , Sun Yan , Shi Feng , Gao Jing , Fu Xing‐Li 
Published: March 2019

AbstractIncreased risk of colorectal cancer (CRC) is associated with altered intestinal microbiota as well as short‐chain fatty acids (SCFAs) reduction of output The energy source of colon cells relies mainly on three SCFAs, namely butyrate (BT), propionate, and acetate, while CRC transformed cells rely mainly on aerobic glycolysis to provide energy. This review summarizes recent research results for dysregulated glucose metabolism of SCFAs, which could be initiated by gut microbiome of CRC. Moreover, the relationship between SCFA transporters and glycolysis, which may correlate with the initiation and progression of CRC, are also discussed. Additionally, this review explores the linkage of BT to transport of SCFAs expressions between normal and cancerous colonocyte cell growth for tumorigenesis inhibition in CRC. Furthermore, the link between gut microbiota and SCFAs in the metabolism of CRC, in addition, the proteins and genes related to SCFAs‐mediated signaling pathways, coupled with their correlation with the initiation and progression of CRC are also discussed. Therefore, targeting the SCFA transporters to regulate lactate generation and export of BT, as well as applying SCFAs or gut microbiota and natural compounds for chemoprevention may be clinically useful for CRCs treatment. Future research should focus on the combination these therapeutic agents with metabolic inhibitors to effectively target the tumor SCFAs and regulate the bacterial ecology for activation of potent anticancer effect, which may provide more effective application prospect for CRC therapy.
Characterization of uropathogenic E. coli O25b‐B2‐ST131, O15:K52:H1, and CGA: Neutrophils apoptosis, serum bactericidal assay, biofilm formation, and virulence typing
Authors: Mostafavi Seyyed Khalil Shokouhi , Najar‐Peerayeh Shahin , Mobarez Ashraf Mohabbati , Parizi Mehdi Kardoust 
Published: March 2019

AbstractPathogenic and drug‐resistant strains of Escherichia coli (E. coli) O25b‐B2‐ST131, O15:H1‐D‐ST393, and CGA (clonal group A) clonal groups have spread worldwide. This study aimed at determining
E. coli epidemic clonal groups, their virulence factors, biofilm formation, neutrophils apoptosis, and antimicrobial resistance pattern of uropathogenic
E. coli. A total of 95 CTX‐M‐1‐producing
E. coli clinical isolates were enrolled.
E. coli O25b‐B2‐ST131, CGA, and O15:K52:H1 were identified by serotyping and phylogrouping and allele‐specific polymerase chain reaction‐based assay. Antibiotic susceptibility, biofilm formation, hemolysis, and human serum bactericidal assay were performed. Neutrophil apoptosis was assayed by flow cytometry. Nine
E. coli clonal groups including six O25b‐B2‐ST131 strains, two CGA, and one O15:K52:H1‐D‐ST393 strains were detected. One O25b‐B2‐ST131 isolate was a strong biofilm‐producer. Three ST131 isolates had type I fimbriae. Furthermore, all the CGA and O15:K52:H1 and three of ST131 isolates harbored the P fimbriae. The virulence genes
ompT,
fimH, and
traT were detected among all the clonal groups. The apoptosis was induced by O25b‐B2‐ST131, CGA, and O15:K52:H1
E. coli. There was no significant difference regarding apoptosis induction among clonal groups. Furthermore, the presence of the
cdt,
usp, and
vat genes was significantly associated with the apoptosis of neutrophils by O25b‐B2‐ST131, CGA, and O15:K52:H1‐D‐ST393 clonal groups.
VEGF inhibition alters neurotrophin signalling pathways and induces caspase‐3 activation and autophagy in rabbit retina
Authors: Segatto Marco , Fico Elena , Gharbiya Magda , Rosso Pamela , Carito Valentina , Tirassa Paola , Plateroti Rocco , Lambiase Alessandro 
Published: March 2019

AbstractThis study sought to evaluate the prospective role exerted by vascular endothelial growth factor (VEGF) in the modulation of nerve growth factor (NGF) and brain‐derived neurotrophic factor (BDNF) signalling pathways in the rabbit retina. To reach this aim, the anti‐VEGF agents aflibercept and ranibizumab were used as a pharmacological approach to evaluate the putative consequences elicited by VEGF inhibition on neurotrophin signalling. VEGF inhibition determined a marked imbalance in proneurotrophin expression, a significant reduction in TrkA and TrkB phosphorylation states and a decrease in the pan‐neurotrophin receptor p75. Importantly, VEGF blockade also caused a strong increase in cleaved caspase‐3, beclin‐1 and lipidated LC3. The effects were more pronounced in the aflibercept group when compared with ranibizumab‐treated rabbits, particularly 1 week after injection. This study demonstrates that VEGF exerts pivotal physiological roles in regulating NGF and BDNF pathways in the retina, as its inhibition by anti‐VEGF agents deeply impacts neurotrophin homeostasis. These events are accompanied by a sustained induction of apoptotic and autophagic markers, suggesting that anti‐VEGF‐dependent impairments in neurotrophin signalling could be responsible for the activation of retinal cell death pathways.
Phosphoglycerate kinase deficiency: A nationwide multicenter retrospective study
Authors: Echaniz‐Laguna Andoni , Nadjar Yann , Béhin Anthony , Biancalana Valérie , Piraud Monique , Malfatti Edoardo , Laforêt Pascal 
Published: March 2019

AbstractPhosphoglycerate kinase (PGK) deficiency is a rare X‐linked metabolic disorder caused by mutations in the PGK1 gene. Patients usually develop various combinations of nonspherocytic hemolytic anemia (NSHA), myopathy, and central nervous system disorders. In this national multicenter observational retrospective study, we recorded all known French patients with PGK deficiency, and 3 unrelated patients were identified. Case 1 was a 32‐year‐old patient with severe chronic axonal sensorimotor polyneuropathy resembling Charcot–Marie–Tooth (CMT) disease, mental retardation, microcephaly, ophthalmoplegia, pes cavus, and the new c.323G > A PGK1 hemizygous mutation. Case 2 was a 71‐year‐old patient with recurrent exertional rhabdomyolysis, and a c.943G > A PGK1 hemizygous mutation. Case 3 was a 48‐year‐old patient with NSHA, retinitis pigmentosa, mental retardation, seizures, stroke, parkinsonism, and a c.491A > T PGK1 hemizygous mutation. This study confirms that PGK deficiency is an extremely rare disorder with a wide phenotypic spectrum, and demonstrates for the first time that PGK deficiency may affect the peripheral nervous system and present as a CMT‐like disorder.
The abdomen of a free‐living female of Strepsiptera and the evolution of the birth organs
Authors: Tröger Daniel , Beutel Rolf G. , Pohl Hans 
Published: March 2019

AbstractMengenillidae is a small, basal family of Strepsiptera, mainly characterized by free‐living females in contrast to the endoparasitic females of Stylopidia. Here, we describe external and internal structures of the female abdomen of Eoxenos laboulbenei (Mengenillidae). The external morphology was examined and documented using microphotography. Internal structures were reconstructed three‐dimensionally using a μCT‐data set. The morphologically simplified abdomen comprises 10 segments. The integument is weakly sclerotized and flexible. Spiracles are present dorsolaterally on segments I–VII. Segment VII bears the posteroventral birth opening and the small abdominal segment X carries the anus at its apex. Numerous eggs float freely in the hemolymph. The musculature of segments I–IV is composed of ventral and dorsal longitudinal muscle bundles, strongly developed paramedial dorsoventral muscles and a complex meshwork of small pleural muscles, with minimal differences between the segments. Segments V–X contain more than 50 individual muscles, even though the musculature as a whole is weakly developed. Even though it is not involved in processing food, the digestive tract is well‐developed. Its postabdominal section comprises a part of the midgut and the short hindgut. The midgut fills a large part of the postabdominal lumen. The lumina of the midgut and hindgut are not connected. Five or six nodular Malpighian tubules open into the digestive tract at the border region between the midgut and hindgut. The birth organ below the midgut releases the primary larvae after hatching via the birth opening at segment VII. It is likely derived from primary female genital ducts. The presence of six additional birth organs of segments I–VI are de novo formations and a groundplan apomorphy of Stylopidia, the large strepsipteran subgroup with endoparasitic females. The loss of the primary birth organ of segment VII is an apomorphy of Stylopiformia (Stylopidia excl. Corioxenidae).
Raman spectra of natural manganese oxides
Authors: Bernardini Simone , Bellatreccia Fabio , Casanova Municchia Annalaura , Della Ventura Giancarlo , Sodo Armida 
Published: March 2019

AbstractManganese oxides occur typically as cryptocrystalline and fine‐grained mixtures of different Mn‐phases, carbonates, silicates, and Fe oxides/hydroxides; thus their characterization by standard methods, such as X‐ray diffraction, is extremely challenging. These materials have been widely used in various applications over the millennia, for example, in art works as pigments for pottery, mural paintings, stained glass, and recently, as nanostructured materials with very attractive physicochemical properties. Furthermore, they are important geomaterials that could play a key role in environmental applications, by controlling the partitioning of arsenic and heavy metals between rocks, soils, and aqueous systems. Raman spectroscopy, which is a punctual and nondestructive technique, has been widely used to characterize these materials. However, literature data are often conflicting and contradictory, usually not allowing a proper identification of the Mn species. In this work, we characterize the most common natural manganese oxides by combining X‐ray powder diffraction, Fourier‐transform infrared spectroscopy, and Raman spectroscopy. Our data show that some manganese oxides have characteristic Raman spectra and can be easily recognized by using Raman spectroscopy alone, whereas integration of Raman data with other techniques is mandatory to characterize minerals that have almost identical Raman spectra. With this respect, Raman spectroscopy is the only technique allowing an easy discrimination between hollandite [Ba(Mn4+6,Mn3+2)O16] and cryptomelane [K(Mn4+7,Mn3+)O16]. The final goal of this work is to provide reference Raman spectra, acquired on previously well‐characterized Mn samples to facilitate the application of Raman spectroscopy in the study of these geomaterials.
Grape seed pomace as a valuable source of antioxidant fibers
Authors: Costa Gislaine NS , Tonon Renata V , Mellinger‐Silva Caroline , Galdeano Melicia C , Iacomini Marcello , Santiago Manuela CPA , Almeida Eveline L , Freitas Suely P 
Published: March 2019

ABSTRACTBACKGROUNDGrape seed pomace is a valuable by‐product from winery/oil extraction industries and a potential source of bioactive compounds. Hot water extraction is the most widely used technology for polysaccharide recovery from plant and seed sources. To obtain an antioxidant fiber‐rich extract (AFE) from defatted grape seed pomace, seed:water ratio (1:10–1:20) and extraction temperature (70–90 °C) effects on extraction yield, total sugars, total phenolic compounds and condensed tannins were investigated.RESULTSThe best extraction results were achieved at the highest seed:water ratio and temperature. At these conditions, the extraction yield and total sugar content of the freeze‐dried AFE were 100.1 g kg−1 and 725.3 g kg−1, respectively. The AFE presented high total phenolic content (16.2 g GAE kg−1), condensed tannins (515.1 mg CAT kg−1), soluble (219.1 g kg−1) and insoluble fibers (132.4 g kg−1), besides protein (171.1 g kg−1) and ash (152.6 g kg−1). The antioxidant capacity of the AFE was 382.7, 823.7 and 1439.4 μmol Trolox g−1 for DPPH, ABTS and ORAC methods.CONCLUSIONThe AFE was shown to be not only an interesting source of fiber with antioxidant capacity, which could become a potential foodstuff, but also an ecofriendly and sustainable alternative to use grape seed pomace. © 2019 Society of Chemical Industry
Phenolic acids variability and grain quality of organically and conventionally fertilised old wheats under a warm climate
Authors: Fares Clara , Menga Valeria , Codianni Pasquale , Russo Mario , Perrone Domenico , Suriano Serafino , Savino Michele , Rascio Agata 
Published: March 2019

AbstractBACKGROUNDWe investigated three old wheats (Triticum dicoccum, Triticum durum, Triticum spelta) for effects of organic and conventional cultivation under equivalent nitrogen fertilisation (100 kg ha−1) on antioxidant content and composition, and on quality traits.RESULTSCompared to conventional cultivation, organic cultivation had positive effects on test weight, while grain quality traits of protein content, gluten content and sodium dodecyl sulphate microsedimentation volume were 19.2%, 9.3% and 22.7% lower, respectively. Despite lower protein content with organic cultivation, this was still high (147 g kg−1); thus with adequate organic nitrogen fertilisation, the quality traits related to the technological properties of flour were maintained. Total phenolic content (TPC) was significantly higher for organic versus conventional cultivation, although free and bound phenolic acids were not significantly different. With discriminant analysis, only durum wheat differentiated the qualitative and compositional traits according to cultivation system. Separation of organic versus conventional cultivation in durum wheat and emmer was strongly affected by antioxidants (antioxidant capacity, TPC, yellow pigment content), dietary fibre, protein content and test weight.CONCLUSIONSWith adequate organic nitrogen fertilisation, protein and gluten contents remain high enough for good quality pasta. However, except for TPC, organic cultivation did not affect phenolic acids profile and antioxidant activity. © 2019 Society of Chemical Industry
A qualitative cognitive appraisal of tourist harassment
Authors: Otoo Felix Elvis , Badu‐Baiden Frank , Kim Seongseop (Sam) 
Published: March 2019

AbstractAlthough hostility against tourists has gained a respectable amount of research attention over the last two decades, tourist harassment has proved to be an elusive topic for researchers. This study makes an important contribution to the literature by demonstrating the multidimensional nature of tourist harassment, with attention paid to the application of cognitive appraisal theory. We argue the need for an in‐depth understanding of victims' perspective of tourist harassment. The study involved 19 on‐site interviews conducted in Ghana. The results show that tourists are subjected to aggressive and passive forms of harassment. The qualitative inquiry revealed that harassment has substantial effects on tourists' cognitive assessment, emotional response, coping strategy, destination image, and future intentions. Expressing annoyance and making behavioral adjustments were the more notable emotional and coping responses recorded. Although respondents were generally confident about recommending Ghana as a destination in spite of the harassment, they were divided in terms of their intentions to revisit the destination.
Impact Statement Coding of Self‐Related Thought in Women With Posttraumatic Stress Disorder
Authors: Philippi Carissa L. , Dahl Gregory , Jany Miranda , Bruce Steven E. 
Published: March 2019

AbstractClinical and empirical studies have documented significant alterations in self‐related thought in posttraumatic stress disorder (PTSD). To our knowledge, however, few studies have examined self‐referential cognition by analyzing survivors’ written impact statements in individuals whose PTSD is due to interpersonal trauma. In the current study, we performed a linguistic analysis of impact statements (i.e., descriptions of how the trauma has affected the survivors’ views of themselves, others, and the world) collected at the start of therapy from women with PTSD that was due to interpersonal trauma, with the goal of determining whether PTSD symptom clusters were associated with altered self‐referential thought. We found that higher levels of PTSD‐related hyperarousal symptoms were significantly associated with diminished self‐related word use, after controlling for depression severity, f2 = .31. Similarly, diminished negative self‐related word use was associated with higher levels of hyperarousal symptoms, f2 = .47. Our findings align with previous research that has provided evidence for a diminished sense of self in PTSD. These results may help inform targeted interventions aimed at improving social‐affective functions in PTSD and other psychiatric conditions.