bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of Hepatitis C Replication by Targeting the Molecular Chaperone Hsp90: Synthesis and Biological Evaluation of 4,5,6,7‐Tetrahydrobenzo[1,2‐d]thiazole Derivatives
Authors: Lillsunde Katja‐Emilia , Tomašič Tihomir , Schult Philipp , Lohmann Volker , Kikelj Danijel , Tammela Päivi 
Published: December 2018

AbstractCellular chaperones that belong to the heat‐shock protein 90 (Hsp90) family are a prerequisite for successful viral propagation for most viruses. The hepatitis C virus (HCV) uses Hsp90 for maturation, folding, and modification of viral proteins. Based on our previous discovery that marine alkaloid analogues with a 4,5,6,7‐tetrahydrobenzo[1,2‐d]thiazole‐2‐amine structure show inhibition of HCV replication and binding to Hsp90, a series of twelve novel compounds based on this scaffold was designed and synthesized. The aim was improved Hsp90 affinity and anti‐HCV activity. Through structural optimization, improved binding to Hsp90 and specific HCV inhibition in genotype 1b and 2a replicon models was achieved for three compounds belonging to the newly synthesized series. Furthermore, these compounds efficiently inhibited replication of full‐length HCV genotype 2a in a reporter virus RNA assay with IC50 values ranging from 0.03 to 0.6 μm.
Renal Chloride Channels in Relation to Sodium Chloride Transport
Authors: Teulon Jacques , Planelles Gabrielle , Sepúlveda Francisco V. , Andrini Olga , Lourdel Stéphane , Paulais Marc 
Published: December 2018

ABSTRACTThe many mechanisms governing NaCl absorption in the diverse parts of the renal tubule have been largely elucidated, although some of them, as neutral NaCl absorption across the cortical collecting duct or regulation through with‐no‐lysine (WNK) kinases have emerged only recently. Chloride channels, which are important players in these processes, at least in the distal nephron, are the focus of this review. Over the last 20‐year period, experimental studies using molecular, electrophysiological, and physiological/functional approaches have deepened and renewed our views on chloride channels and their role in renal function. Two chloride channels of the ClC family, named as ClC‐Ka and ClC‐Kb in humans and ClC‐K1 and ClC‐K2 in other mammals, are preponderant and play complementary roles: ClC‐K1/Ka is mainly involved in the building of the interstitial cortico‐medullary concentration gradient, while ClC‐K2/Kb participates in NaCl absorption in the thick ascending limb, distal convoluted tubule and the intercalated cells of the collecting duct. The two ClC‐Ks might also be involved indirectly in proton secretion by type A intercalated cells. Other chloride channels in the kidneys include CFTR, TMEM16A, and probably volume‐regulated LRRC8 chloride channels, whose function and molecular identity have not as yet been established. © 2019 American Physiological Society.Compr Physiol9:301‐342, 2019.
Telemedicine for screening diabetic retinopathy: The NO BLIND Italian multicenter study
Authors: Sasso Ferdinando Carlo , Pafundi Pia Clara , Gelso Aldo , Bono Valeria , Costagliola Ciro , Marfella Raffaele , Sardu Celestino , Rinaldi Luca , Galiero Raffaele , Acierno Carlo , de Sio Chiara , Adinolfi Luigi Elio ,   on behalf of NO BLIND Study Group
Published: December 2018

AbstractAimsDiabetic retinopathy (DR) represents the main cause of blindness among adults in the industrialized Countries. Use of telemedicine could offer an easy, smart specialist fundus oculi examination, as well as putting in a screening programme many patients who otherwise would be excluded.Materials and methodsThe NO BLIND is a transversal, multicentre, observational study. Its pilot phase involved nine public outpatient clinics for 6 months. As endpoint of the study, we assessed the prevalence of DR by retinography in a subset of the Italian population. Patients' fundus oculi photos were performed by trained diabetologists through a digital smart ophthalmoscope.ResultsAccording to our endpoint, in the final study population (n = 1461), obtained excluding patients for whom retinography was not able to provide any diagnosis, DR prevalence was equal to 15.5%. According to the receiver operating characteristic (ROC) curve performed, we can observe how retinography appears a highly accurate method to detect DR (AUROC 0.971, 95% confidence interval, 0.954‐0.989), with a specificity of the 100% and a sensitivity of the 94.3%.ConclusionsOur findings, in an Italian setting, confirm main data in the literature about DR prevalence. Hence, telemedicine could represent an accurate, fast, and cheap method for screening of DR.
Microfluidic techniques for tumor cell detection
Authors: Tan Feifei , Wang Tianbao , Wang Haishi , Zheng Yuzheng 
Published: December 2018

AbstractCancer metastasis is the main cause of cancer‐related death. Early detection of tumor cell in peripheral blood is of great significant to early diagnosis and effective treatment of cancer. Over the past two decades, microfluidic technologies have been demonstrated to have great potential for isolating and detecting tumor cell from blood. The present paper reviews microfluidic techniques for tumor cell detection based on various physical principles. The specific methods are categorized into active and passive methods depending on whether extra force field is applied. Working principles of the two methods are explained in detail, including microfluidics combined with optical tweezer, electric field, magnetic field, acoustophoresis, and without extra fields for tumor cell detection. Typical experiments and the results are reviewed. Based on these, research characteristics of the two methods are analyzed.
Impact of sex and reproductive status on memory circuitry structure and function in early midlife using structural covariance analysis
Authors: Seitz Johanna , Kubicki Marek , Jacobs Emily G. , Cherkerzian Sara , Weiss Blair K. , Papadimitriou George , Mouradian Palig , Buka Stephen , Goldstein Jill M. , Makris Nikos 
Published: December 2018

AbstractResearch on age‐related memory alterations traditionally targets individuals aged ≥65 years. However, recent studies emphasize the importance of early aging processes. We therefore aimed to characterize variation in brain gray matter structure in early midlife as a function of sex and menopausal status. Subjects included 94 women (33 premenopausal, 29 perimenopausal, and 32 postmenopausal) and 99 demographically comparable men from the New England Family Study. Subjects were scanned with a high‐resolution T1 sequence on a 3 T whole body scanner. Sex and reproductive‐dependent structural differences were evaluated using Box's M test and analysis of covariances (ANCOVAs) for gray matter volumes. Brain regions of interest included dorsolateral prefrontal cortex (DLPFC), inferior parietal lobule (iPAR), anterior cingulate cortex (ACC), hippocampus (HIPP), and parahippocampus. While we observed expected significant sex differences in volume of hippocampus with women of all groups having higher volumes than men relative to cerebrum size, we also found significant differences in the covariance matrices of perimenopausal women compared with postmenopausal women. Associations between ACC and HIPP/iPAR/DLPFC were higher in postmenopausal women and correlated with better memory performance. Findings in this study underscore the importance of sex and reproductive status in early midlife for understanding memory function with aging.
Shared and distinct neural circuitry for nonsymbolic and symbolic double‐digit addition
Authors: Bugden Stephanie , Woldorff Marty G. , Brannon Elizabeth M. 
Published: December 2018

AbstractSymbolic arithmetic is a complex, uniquely human ability that is acquired through direct instruction. In contrast, the capacity to mentally add and subtract nonsymbolic quantities such as dot arrays emerges without instruction and can be seen in human infants and nonhuman animals. One possibility is that the mental manipulation of nonsymbolic arrays provides a critical scaffold for developing symbolic arithmetic abilities. To explore this hypothesis, we examined whether there is a shared neural basis for nonsymbolic and symbolic double‐digit addition. In parallel, we asked whether there are brain regions that are associated with nonsymbolic and symbolic addition independently. First, relative to visually matched control tasks, we found that both nonsymbolic and symbolic addition elicited greater neural signal in the bilateral intraparietal sulcus (IPS), bilateral inferior temporal gyrus, and the right superior parietal lobule. Subsequent representational similarity analyses revealed that the neural similarity between nonsymbolic and symbolic addition was stronger relative to the similarity between each addition condition and its visually matched control task, but only in the bilateral IPS. These findings suggest that the IPS is involved in arithmetic calculation independent of stimulus format.
Factors associated with gastrostomy tube dependence following salvage total laryngectomy with microvascular free tissue transfer
Authors: Worley Mitchell L. , Graboyes Evan M. , Blair Julie , Momin Suhael , Day Terry A. , Hornig Joshua D. , Skoner Judith , Huang Andrew T. 
Published: December 2018

AbstractBackgroundFollowing salvage total laryngectomy (STL) with microvascular‐free tissue transfer (MFTT), patients are at high risk for swallowing dysfunction, but risk factors for persistent gastrostomy tube (G‐tube) dependence are unknown.MethodsRetrospective review of 33 patients who underwent STL with MFTT.ResultsA total oral diet was achieved by 81% of patients with ≥6 months of postoperative follow‐up. Approximately 27% of patients were G‐tube dependent preoperatively with 67% achieving a total oral diet postoperatively. Factors associated with persistent G‐tube dependence included pT4 tumor, pN2+ status, more extensive pharyngectomy, and re‐irradiation. Strictures occurred in 30% of patients and were associated with more extensive pharyngectomy and tubed reconstruction.ConclusionsFor patients undergoing STL with MFTT, the majority of patients achieve a total oral diet regardless of their preoperative swallowing function. Advanced‐stage recurrent tumors and increased extent of pharyngectomy contribute to poorer swallowing outcomes.
Translation and validation of the MD Anderson Dysphagia Inventory (MDADI) for Spanish‐speaking patients
Authors: Montes‐Jovellar Lourdes , Carrillo Alvaro , Muriel Alfonso , Barbera Rafael , Sanchez Fatima , Cobeta Ignacio 
Published: December 2018

AbstractBackgroundThe main objective of this study was to perform the adaptation and cultural translation and validation of the MD Anderson Dysphagia Inventory (MDADI) questionnaire for the Spanish language.MethodsA total of 69 patients were diagnosed with head and neck cancer treated with surgery; radiotherapy and chemoradiotherapy were included. MDADI was translated and a feasibility, internal consistency, test‐retest reliability, and construct validity were assessed.ResultsThe mean overall score of the MDADI was 51.9 (18‐85). Internal consistency for total score was 0.908. The overall score of intraclass correlation coefficient was 0.98 and kappa coefficient scores were almost perfect (test‐retest reliability). All domains of MDADI were significantly correlated with physical and mental domains of the SF‐12. Construct validity was also evaluated with food texture measures, and with TNM classification.ConclusionThe translation and validation of the Spanish version of the MDADI was performed and can be considered an important patient‐reported outcomes tool for dysphagia‐related quality of life.
18F‐FDG PET/MRI vs MRI in patients with recurrent adenoid cystic carcinoma
Authors: Kirchner Julian , Schaarschmidt Benedikt M. , Sauerwein Wolfgang , Deuschl Cornelius , Arweiler‐Harbeck Diana , Holtmann Laura , Stebner Vanessa , Umutlu Lale , Antoch Gerald , Ruhlmann Verena 
Published: December 2018

AbstractObjectivesTo evaluate and compare the diagnostic potential of 18F‐fluorodeoxyglucose positron emission tomography/magnetic resonance imaging (18FDG‐PET/MRI) and MRI for recurrence diagnostics after primary therapy in patients with adenoid cystic carcinoma (ACC).MethodsA total of 32 dedicated head and neck 18F‐FDG PET/MRI datasets were included in this analysis. MRI and 18F‐FDG PET/MRI datasets were analyzed in separate sessions by two readers for tumor recurrence or metastases.ResultsLesion‐based sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy were 96%, 84%, 90%, 93%, and 91% for 18F‐FDG PET/MRI and 77%, 94%, 95%, 73%, and 84% for MRI, resulting in a significantly higher diagnostic accuracy of 18F‐FDG PET/MRI compared to MRI (P < .005).Conclusion
18F‐FDG PET/MRI is superior to MRI in detecting local recurrence and metastases in patients with ACC of the head and neck. Especially concerning its negative predictive value, 18F‐FDG PET/MRI outperforms MRI.
Reimbursement pricing for new medical devices in Japan: Is the evaluation of innovation appropriate?
Authors: Tamura Makoto , Nakano Shohei , Sugahara Takuma 
Published: December 2018

SummaryObjectivesIn Japan, strong reimbursement pricing control measures for existing medical device products have rendered new medical device reimbursement pricing critical for manufacturers. Few studies have been conducted on this aspect; therefore, this paper (1) clarifies whether evaluation of innovation is appropriate or not and (2), if not, investigates its background.MethodsIn this research, 319 C1/C2 government decisions for new medical devices in the 10 years from April 2008 to March 2018 were analyzed. Evaluation of innovation was considered in terms of the reimbursement price, as well as the foreign average price ratio.ResultsConsidering the degree of evaluation of innovation, the average premium rate for the similar function category comparison method was 10.2% during 2008 to 2010 (this means the newly set reimbursement price was 10.2% higher than that of corresponded exiting categories); it declined consistently thereafter, to 3.2% during 2016 to 2018. Moreover, evaluation of innovation by the foreign average price (FAP) ratio was 1.04 in 2008 to 2010, consistently decreasing to 0.88 in 2016 to 2018. The period from product approval to the non‐Special Designated Treatment Material (non‐STM) (a part of technical fee) price listing is much longer than that of the STM (own reimbursement price) listing.ConclusionSeveral reasons were considered for the decline in innovation evaluation: (1) the lowering of the FAP upper limit ratio, (2) the possibility that there was not enough evidence at the time of price listing, (3) and the more rigorous standards to create a new separate functional category. However, some aspects were attributable to reimbursement system reform.
miR‐150‐504‐519d inhibits the growth of human colorectal cancer cell line SW48 and downregulates c‐FLIP receptor
Authors: Rong Guoqiang , Yang Xiaodong , Wu Haorong , Wu Yongyou 
Published: December 2018

AbstractmicroRNAs (miRNAs) are noncoding RNAs that regulates the expression of target messenger RNAs (mRNAs). c‐FLIP is an inhibitor of cell apoptosis through inhibition of caspase 8. miR‐150, miR‐504, and miR‐519d were related to cancer cell proliferation, invasion, and migration in colorectal cancer (CRC). However, the role of miR‐150‐504‐519d in CRC has not been studied and the relationship between miR‐150‐504‐519d and c‐FLIP remains unclear. In this study, we found that c‐FLIP was upregulated in CRC tissues, without detectable expression in normal CRC tissues. Using SW48 cell line, we further showed that miR‐150‐504‐519d inhibited migration, invasion, and promoted apoptosis of SW48 cells. Moreover, in SW48 cell line transfected with miR‐150‐504‐519d, the protein expression of c‐FLIP was significantly lower compared with cells transfected with scramble. Our results demonstrated upregulation of c‐FLIP in CRC, which was downregulated in SW48 cells after the transfection of miR‐150‐504‐519d, suggesting that manipulation of miR‐150‐504‐519d expression might be a novel approach for the treatment of colorectal cancer.
Mandibular osteotomy‐induced hypoxia enhances osteoclast activation and acid secretion by increasing glycolysis
Authors: Tang Yi , Zhu Jie , Huang Danqing , Hu Xiu , Cai Yun , Song Xiwen , Song Zhaohui , Hong Chaoyue , Feng Zifan , Kang Feiwu 
Published: December 2018

AbstractThe rapid bone remodeling after osteotomy has been reported for a long time. However, the underlying mechanism promoting the active bone reconstruction was still to be elucidated. Since not only the bone, blood vessels, and supportive tissues, but also the local microenvironment were destroyed, if the changes on the cell metabolism was contributed to the accelerated bone remodeling came into sight. In present study, we found that the mandibular osteotomy in rabbit activated osteoclasts, as well as the expression of hypoxia‐inducible factor 1α (HIF‐1α) in alveolar bone. Hypoxia or HIF‐1α could enhanced osteoclastogenesis, bone absorption, and lactic acid concentration in receptor activator of nuclear factor κΒ ligand‐induced RAW264.7 cells. Coincided with the upregulated HIF‐1α expression, HIF‐driven glycolytic enzymes, such as lactate dehydrogenase A (LDHA), glucokinase (GCK), pyruvate kinase M2 (PKM2), and phosphofructokinase1 (PFK1), were found massively increased in both hypoxic RAW264.7 cells and the alveolar HIF‐1α‐positive osteoclasts after mandibular osteotomy. Knockdown of HIF‐1α suppressed not only the hypoxia‐mediated glycolysis, but also the hypoxia‐induced acid secretion and bone resorption in RAW264.7 cells. Application of inhibitor on glycolysis gave rise to the similar results as HIF‐1α knockdown. Our findings suggested that hypoxia‐driven glycolysis in osteoclasts was an adaptive mechanism to permit alveolar bone remodeling after mandibular osteotomy.
Downregulated microRNA‐330 suppresses left ventricular remodeling via the TGF‐β1/Smad3 signaling pathway by targeting SRY in mice with myocardial ischemia–reperfusion injury
Authors: Liu Zheng‐Yu , Pan Hong‐Wei , Cao Yan , Zheng Jiao , Zhang Yu , Tang Yi , He Jin , Hu Yong‐Jun , Wang Chang‐Lu , Zou Qiong‐Chao , Fu Qing‐Hua , Zhang Le , Peng Jian‐Qiang , Ling Jing , Peng Ning , Rong Jing‐Jing , Zheng Zhao‐Fen 
Published: December 2018

AbstractmicroRNAs (miRs) are essential in the development of heart failure. The aim of this study is to investigate the effect of microRNA‐330 (miR‐330) on left ventricular remodeling via the TGF‐β1/Smad3 signaling pathway by targeting the sex‐determining region Y (SRY) in mice with myocardial ischemia–reperfusion injury (MIRI). Differentially expressed gene (DEG) in myocardial ischemia–reperfusion (IR) was screened out and the miR that targeted the DEG was also predicted and verified. A model of MIRI was established to detect the expression of miR‐330, SRY, transforming growth factor‐β (TGF‐β1), and Sekelsky mothers against dpp3 (Smad3). To further investigate the role of miR‐330 in MIRI with the involvement of SRY and TGF‐β1/Smad3 signaling pathway, the modeled mice were treated with different mimic, inhibitor, or small interfering RNA (siRNA) to observe the changes of the related gene expression, as well as the myocardial infarction size and volume of myocardial collagen. SRY was screened out and verified as a target gene of miR‐330. The MIRI mice showed enlarged myocardial infarction size, increased volume of myocardial collagen, increased expression of miR‐330, TGF‐β1 and Smad3, while decreased the expression of SRY. The MIRI mice treated with miR‐330 inhibitor showed decreased myocardial infarction size, the volume of myocardial collagen, and expression of TGF‐β1 and Smad3 but promoted expression of SRY. Our findings demonstrated that downregulated miR‐330 could suppress left ventricular remodeling to inhibit the activation of the TGF‐β1/Smad3 signaling pathway via negatively targeting of SRY in mice with MIRI. This can be a potential target in the strategy to attenuate patient suffering.
Mycobacterium avium complex infection in patients with human immunodeficiency virus: A systematic review and meta‐analysis
Authors: Heidary Mohsen , Nasiri Mohammad Javad , Mirsaeidi Mehdi , Jazi Faramarz Masjedian , Khoshnood Saeed , Drancourt Michel , Darban‐Sarokhalil Davood 
Published: December 2018

AbstractBackgroundMycobacterium avium‐intracellulare complex (MAC) is one of the leading causes of death among people living with human immunodeficiency virus (HIV). The current study was aimed to determine the frequency of MAC infection in patients infected with HIV.MethodsEmbase, PubMed, and Web of Science were searched for relevant studies. All statistical analyses were performed by STATA version 14.ResultsFrom 6,627 retrieved articles, 23 were included in the final analysis. A total of 18,463 patients with HIV were included in the analysis. The frequency of MAC infection in patients with HIV was found to be 10.6% (95% confidence interval, 6.9–14.2).ConclusionThe relatively large fractions of HIV‐infected patients were coinfected with MAC, which may poses significant public health problems. Continued progress in the development of rapid diagnostic methods and preventive therapy for MAC should lead to further improvements in survival and quality of life in patients with HIV.
Spatiotemporal variability in seawater carbon chemistry for a coral reef flat in Kāne‘ohe Bay, Hawai‘i
Authors: Page Heather N. , Courtney Travis A. , De Carlo Eric H. , Howins Noah M. , Koester Irina , Andersson Andreas J. 
Published: December 2018

AbstractCoral reef community composition and ecosystem function may change in response to anthropogenic ocean acidification. However, the magnitude of acidification on reefs will be modified by natural spatial and temporal variability in seawater CO2 chemistry. Consequently, it is necessary to quantify the ecological, biogeochemical, and physical drivers of this natural variability before making robust predictions of future acidification on reefs. In this study, we measured temporal and spatial physiochemical variability on a reef flat in Kāne‘ohe Bay, O‘ahu, Hawai‘i, using autonomous sensors at sites with contrasting benthic communities and by sampling surface seawater CO2 chemistry across the reef flat at different times of the day during June and November. Mean and diurnal temporal variability of seawater CO2 chemistry was more strongly influenced by depth gradients (~ 0.5–10 m) on the reef rather than benthic community composition. Spatial CO2 chemistry gradients across the reef flat reflected the cumulative influence from benthic metabolism, bathymetry, and hydrodynamics. Based on graphical assessment of total alkalinity–dissolved inorganic carbon data, reef metabolism in November was dominated by organic carbon cycling over inorganic carbon cycling, while these processes were closely balanced in June. Overall, this study highlights the strong influence of depth on reef seawater CO2 chemistry variability through its effects on benthic biomass to seawater volume ratio, seawater flow rates, and residence time. Thus, the natural complexity of ecosystems where a combination of ecological and physical factors influence reef chemistry must be considered when predicting ecosystem biogeochemical responses to future anthropogenic changes in seawater CO2 chemistry.