bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functionalized silk fibers from transgenic silkworms for wound healing applications: Surface presentation of bioactive epidermal growth factor
Authors: Wöltje Michael , Böbel Melanie , Bienert Michaela , Neuss Sabine , Aibibu Dilibaier , Cherif Chokri 
Published: May 2018

AbstractGrowth factors play a crucial role in wound healing in general and are promising tools for the treatment of chronic wounds as they can restore the physiological wound healing process. In growth factor‐loaded wound dressings, human epidermal growth factor (EGF) is released in a burst and washed out quickly. The developed matrix consists of recombinant EGF produced in transgenic silkworms as a fusion protein with the fibroin light chain. The covalent linkage prevents EGF from draining into the surrounding tissue while presenting the growth factor on the surface. EGF‐functionalized silk membranes and nonwovens lead to a 2.5‐fold increase in the cell number of fibroblasts, while retaining full bioactivity even after e‐beam sterilization. EGF is long‐term presented without burst release and significantly reduces the wound area by 15% in an in vitro wound model. Hence, the cost‐effective production of a biomaterial using transgenic silkworm larvae in combination with a growth factor paves the way for a promising new multifactorial wound cover for chronic wound healing. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2643–2652, 2018.
Effect of OATP1B1/1B3 Inhibitor GDC‐0810 on the Pharmacokinetics of Pravastatin and Coproporphyrin I/III in Healthy Female Subjects
Authors: Liu Lichuan , Cheeti Sravanthi , Yoshida Kenta , Choo Edna , Chen Eugene , Chen Buyun , Gates Mary , Singel Stina , Morley Roland , Ware Joseph , Sahasranaman Srikumar 
Published: May 2018

AbstractDeveloped as an oral anticancer drug to treat estrogen receptor–positive breast cancer, GDC‐0810 was shown to be a potent inhibitor of organic anion‐transporting polypeptide 1B1 and 1B3 (OATP1B1/1B3) from an in vitro assay. A clinical study was conducted to assess the drug‐drug interaction potential between GDC‐0810 and pravastatin, which is a relatively selective and sensitive OATP1B1/1B3 substrate. Fifteen healthy female subjects of non‐childbearing potential were enrolled in the study. On day 1 in period 1, a single 10‐mg dose of pravastatin was administered to all subjects. Following a 4‐day washout period, 600 mg of GDC‐0810 was administered once daily on days 5 through 8 in period 2 to achieve steady‐state concentrations. On day 7, a single dose of 10‐mg pravastatin was coadministered with the 600‐mg GDC‐0810 dose. Concentrations of pravastatin (periods 1 and 2) and GDC‐0810 (period 2 only) were quantified in blood samples and subsequently used to calculate the pharmacokinetics (PK) parameters. The pravastatin mean maximal concentration and area under the curve values were approximately 20% and 41% higher, respectively, following pravastatin coadministration with GDC‐0810 compared to pravastatin alone. Based on the magnitude of change in this drug‐drug interaction study, dose adjustments for pravastatin (and other OATP1B1/1B3 substrates) were not considered necessary when administered with GDC‐0810. Retrospectively, the endogenous biomarkers of OATP1B1/1B3, coproporphyrin I and III, were also measured and showed changes comparable to those of pravastatin, indicating their utility in detecting weak inhibition of OATP1B1/1B3 in the clinical setting.
Changes in nano‐mechanical properties of human epidermal cornified cells depending on their proximity to the skin surface
Authors: Milani Pascale , Chlasta Julien , Abdayem Rawad , Kezic Sanja , Haftek Marek 
Published: May 2018

AbstractDuring formation of the stratum corneum (SC) barrier, terminally differentiated keratinocytes continue their maturation process within the dead superficial epidermal layer. Morphological studies of isolated human corneocytes have revealed differences between cornified envelopes purified from the deep and superficial SC. We used atomic force microscopy to measure the mechanical properties of native human corneocytes harvested by tape‐stripping from different SC depths. Various conditions of data acquisition have been tested and optimized, in order to obtain exploitable and reproducible results. Probing at 200 nN allowed us to investigate the total stiffness of the cells (at 50 nm indentation) and that of the cornified envelopes (at 10 to15 nm), and lipid envelopes (at 5 to 10 nm). The obtained data indicated statistically significant differences between the superficial (more rigid) and deep (softer) corneocytes, thus confirming the existence of depth and maturation‐related morphological changes within the SC. The proposed approach can be potentially used for minimally invasive evaluation of various skin conditions such as aging, skin hydration, and pathologies linked to SC.
Periodontitis and placental growth factor in oral fluids are early pregnancy predictors of gestational diabetes mellitus
Authors: Chaparro Alejandra , Zúñiga Edgardo , Varas‐Godoy Manuel , Albers Daniela , Ramírez Valeria , Hernández Marcela , Kusanovic Juan Pedro , Acuña‐Gallardo Stephanie , Rice Gregory , Illanes Sebastián E. 
Published: May 2018

AbstractBackgroundGestational diabetes mellitus (GDM) affects around 7% to 10% of all pregnancies. Early detection of predisposition to GDM is the first step in developing efficacious preventive treatment. The objective of the present study was to establish the utility of placental proteins presents in oral fluids (gingival crevicular fluid [GCF] and saliva), and periodontal disease status as early pregnancy predictors of GDM.MethodsA nested case control within a prospective cohort was conducted. Pregnant systemically healthy women, aged between 18 and 40 years at 11 to 14 weeks gestation were included. Samples of oral fluids were collected and a complete maternal/obstetric and periodontal history was obtained. The concentration of placental growth factor (PlGF) and soluble Fms‐like tyrosine kinase 1 (sFlt‐1) were measured by enzyme‐linked immunosorbent assay in a nested case control sample of the prospective cohort. Multiple logistic regression models assessed the association. The evaluation of the diagnostic accuracy of the biomarkers was performed through receiver operating characteristic (ROC) curves by calculating the area under the curve (AUC).ResultsThere were recruited 212 pregnant women at 11 to 14 weeks of pregnancy, of these, 14 women (i.e., 6.6%) developed GDM, and displayed significant greater bleeding on probing (BOP) [P = 0.0003]; periodontal probing depth (PD) [P = 0.0028]; clinical attachment level (AL) [P = 0.0008] and periodontal inflamed surface area (PISA) [P = 0.0001]. Similarly, initial glycemia and GCF‐PlGF concentrations were significantly greater in women with GDM [P = 0.0012, and P = 0.0019, respectively]. When data were subjected to ROC curve analysis, the combination of initial glycemia and GCF‐PlGF concentration delivered an area under the ROC curve of 0.897. Multiple logistic regression analyses demonstrate an association between glycemia (OR 1.21, 95% confidence interval [CI] 1.06 to 1.38; P = 0.005) and GCF‐PlGF concentrations in women who developed GDM (OR 1.68, CI 1.05 to 2.68 P = 0.03).ConclusionsWithin the limitations of the present study, the results support that first trimester maternal glycemia combined with GCF‐PlGF concentrations could be a surrogate biomarker for the future development of GDM in pre‐symptomatic women.
MET in gastric cancer with liver metastasis: The relationship between MET amplification and Met overexpression in primary stomach tumors and liver metastasis
Authors: Kim Han S. , Chon Hong J. , Kim Hyunki , Shin Su‐Jin , Wacheck Volker , Gruver Aaron M. , Kim Jong S. , Rha Sun Y. , Chung Hyun C. 
Published: May 2018

Background and ObjectivesAlthough MET amplification/overexpression was observed in a subset of gastric cancer (GC) patients, the relationship between MET amplification/overexpression in primary GC and liver metastasis was unclear.MethodsGC samples and matched liver metastases (N = 47) were analyzed by fluorescence/silver in‐situ hybridization (FISH/SISH) and by immunohistochemistry for MET amplification and MET expression, respectively. MET‐copy number (CN) and Met expression data from The Cancer Genome Atlas Stomach Adenocarcinoma (TCGA‐STAD, N = 356) were also analyzed.ResultsSignificant overlap existed between MET amplification and Met expression in both primary stomach tumors (P = 0.013) and liver metastasis (P = 0.001). In TCGA‐STAD, MET‐CN (≥4 copies) and MET expression were also positively correlated (r = 0.761; P = 0.017). Comparative analysis revealed a strong association between MET expression and MET amplification (85% concurrence) in primary stomach tumors and matched liver metastasis. MET status in synchronous liver metastasis (N = 36) was correlated with primary stomach tumors. However, a significant correlation between primary tumors and liver metastases was not observed in patients with metachronous liver metastasis. Survival analyses revealed that both MET amplification and MET overexpression were prognostic of poor outcomes.ConclusionsMET amplification and Met overexpression were positively correlated in GC. MET status should be re‐evaluated in GC patients with liver metastasis, especially for metachronous metastasis.
Application of denaturing capillary electrophoresis for the detection of prognostic mutations in isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 genes in brain tumors
Authors: Benesova Lucie , Belsanova Barbora , Kramar Filip , Halkova Tereza , Benes Vladimir , Minarik Marek 
Published: May 2018

AbstractMalignant transformation in gliomas is frequently supplemented by somatic mutations in isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 genes. It has recently emerged that mutations in these genes are associated with prolonged survival and should be used as prognostic factor in management of brain cancer patients. There are several approaches in use for the detection of isocitrate dehydrogenase 1 and 2 mutations; however, these often exhibit shortcomings such as convoluted protocols with long processing time, complex (and costly) dedicated fluorescent probes, and/or demand on amounts of input DNA. Therefore, a simple and rapid method would be highly desired. Here, we present development and validation of simple and reliable isocitrate dehydrogenase 1 and 2 mutation detection assay using denaturing capillary electrophoresis. The detection sensitivity in terms of the limiting mutated allele fraction detectable estimated from a series of dilution runs was 2.9%. The method was validated by comparing to results obtained by a widely accepted detection technique, the multiplex ligation‐dependent probe amplification, on a set of 85 brain tumors. The concordance of both methods was 100%, but denaturing capillary electrophoresis assay required fivefold lower input of DNA (1 versus 5 μL of DNA at concentrations typically between 10 and 30 ng/μL).
Predictors of Treatment Completion for Families Referred to Trauma‐Focused Cognitive Behavioral Therapy After Child Abuse
Authors: Celano Marianne , NeMoyer Amanda , Stagg Anna , Scott Nikia 
Published: May 2018

AbstractDespite advances in the dissemination of evidence‐based therapy for abuse‐related traumatic stress, many referred children fail to complete treatment. Using archival data from a sample of children participating in trauma‐focused cognitive behavioral therapy (TF‐CBT) at a hospital‐based child advocacy center, analyses explored the impact of baseline child traumatic stress symptoms, a second (nonprimary) caregiver's treatment attendance, and the number of assessment sessions on treatment completion while controlling for demographic variables. We conducted analyses separately for the total sample (n = 77) and for a subsample of children 6 years of age or older (n = 65) who completed measures of traumatic stress. Families who completed TF‐CBT had fewer pretreatment assessment sessions, odds ratio (OR) = 0.41, 95% CI [0.19, 0.88], and greater nonprimary caregiver session attendance, OR = 1.30, 95% CI [1.03, 1.64], than families who did not complete treatment. Child age, race, and insurance status did not predict treatment completion. Among children at least 6 years of age, treatment completion was related to younger child age, OR = 0.76, 95% CI [0.59, 0.98], and fewer diagnostic evaluation sessions, OR = 0.29, 95% CI [0.11, 0.74], but not to baseline traumatic stress symptoms. Findings may suggest benefits of shortening the assessment period and including a second caregiver in TF‐CBT.
Behavioral response of canada geese to egg‐oiling and nest removal
Authors: Beaumont Matthieu , Rodrigue Jean , Pilotte Catherine , Chalifour Émilie , Giroux Jean‐François 
Published: May 2018

ABSTRACTControlling overabundant goose populations remains a wildlife management challenge. Although reducing adult survival is considered more effective than reducing fecundity, egg‐oiling, and egg removal are often preferential techniques for controlling temperate nesting Canada geese (Branta canadensis) in urban and peri‐urban settings. Little is known about the effect of these methods on the subsequent behavior of geese and their potential effects on population dynamics. We aimed to determine how long Canada geese nesting in southern Quebec, Canada remain at the nest site following egg‐oiling, whether birds submitted to egg‐oiling or nest removal persist in the area to molt and whether they return to breed in the study area the following year, and if so, how far do they nest from their previous nest location. We studied 996 nests initiated by 442 neck‐collared females between 2006 and 2012 including 76 that were oiled, 91 that were removed, 143 that suffered complete loss of the clutch by predators, were flooded, or abandoned, and 686 that were successful. We found that egg‐oiling delayed nest abandonment past the expected hatching date and that the delay increased when applying the treatment later in incubation. We observed only 10 cases of renesting and most occurred after nest flooding. Females that had their nests removed or whose nest failed had a greater probability of leaving the study area to molt elsewhere than females that were successful or those that had their eggs treated with oil. The probability that a female survived and returned to nest in the area was not related to the fate of its nest in the previous year and was estimated at 0.71 (95% CI = 0.67–0.74). The mean distance between successive nesting attempts was not related to the previous year nest fate and averaged 78 ± 8 m. Nest success and thus recruitment was reduced during years with the experimental treatments, but strong philopatry indicates that treatments must be repeated every year to have an effect on the local population growth. If the goal is to keep the geese longer at their nest site, then coating eggs with oil as late as possible in incubation will result in geese remaining at the nest site well past the expected hatch date. If the goal is to cause the geese to leave the nest site, then nest removal should be favored. © 2018 The Wildlife Society.
Effective Enzyme Coimmobilization and Synergistic Catalysis on Hierarchically Porous Inorganic/Organic Hybrid Microbeads Fabricated Via Droplet‐Based Microfluidics
Authors: Cao Xiaodong , Li Wenxiu , Fan Yimei , Dong Hua 
Published: May 2018

AbstractIn this paper, a novel and robust droplet‐based microfluidic method to fabricate poly(ε‐caprolactone)/silica (PCL/SiO2) hybrid microbeads with hierarchically porous architecture is described and their performance as multienzyme carriers for cascade catalysis is further investigated in detail. In addition to the precise control on size and monodispersity of PCL/SiO2 microbeads enabled by the microfluidic method, the presence of ammonia as a catalyst for the hydrolysis and condensation of tetraethylorthosilicate makes it possible to manipulate the competition between sol–gel process and solvent extraction and thus adjust the surface porosity of hybrid microbeads, which eliminates the use of porogens/templates and also the complicated post‐treatment. Isothiocyanate‐immunoglobulin G/cyanine 3‐bovine serum albumin (FITC‐IgG/Cy3‐BSA) and superoxide dismutase/chloramphenicol acetyltransferase (SOD/CAT) are coimmobilized, respectively onto hierarchically porous PCL/SiO2 hybrid microbeads via either physical adsorption or covalent binding. Fluorescence intensity of coimmobilized FITC‐IgG/Cy3‐BSA proves that the proteins/enzymes immobilization amount via covalent binding is much higher than physical adsorption. The enhanced enzymatic activity, total antioxidant capacity, and reusability assay reveal that coimmobilized SOD/CAT exhibits better performance compared with the mono‐immobilized ones, mainly due to their mutual synergistic effect. The excellent results achieved in the work indicate that hierarchically porous PCL/SiO2 hybrid microbeads are very promising carriers for multienzymatic catalysis.
Visible Light–Induced Supra‐Amphiphilic Switch Leads to Transition from Supramolecular Nanosphere to Nanovesicle Activated by Pillar[5]arene‐Based Host–Guest Interaction
Authors: Li Pengyu , Yao Qianfang , Lü Baozhong , Ma Guiping , Yin Meizhen 
Published: May 2018

AbstractA photoresponsive host–guest supramolecular complex (WP5⊃G) constructed by water‐soluble pillar[5]arene (WP5) and spiropyran derivative (G) is presented. The spontaneous isomerization of G from spiropyran (SP) form to ring‐opened merocyanine (MC) form happens either alone or in WP5⊃G in aqueous media. Irradiated by visible light, G can be converted into SP form completely and the hydrophilicity will be changed. G and WP5⊃G are both verified to self‐assemble into nanospheres. Upon exposure to visible light, WP5⊃G reassemble into nanovesicles due to the change of supra‐amphiphilicity, while G alone does not have this transition. Obviously, WP5 takes the key role that activates the photoinduced morphological transition.
The distinctive nucleation of polystyrene composites with differently shaped carbon‐based nanoparticles as nucleating agent in the supercritical CO2 foaming process
Authors: Xiao Wei , Liao Xia , Li Shaojie , Xiong Jian , Yang Qi , Li Guangxian 
Published: May 2018

AbstractMicrocellular polystyrene (PS)/carbon‐based nanoparticle (CbN) composites were prepared by a pressure release process using supercritical CO2 as the foaming agent. Nanocomposite foams with different shapes or dimensions but with CbNs with a similar surface structure showed a quite different cell nucleation. The underlying nucleation mechanism was semi‐quantitatively analyzed by classical nucleation theory, which indicated that the energy barrier for heterogeneous nucleation was related to the shapes of the fillers. Below 80 °C and 20 MPa, the nucleation density of the composites with different fillers showed marked differences, among which the nucleation density of PS/fullerene (FE) was the highest, followed by PS/carbon nanotubes (CNTs) and PS/thermally reduced graphene (TRG). However, the difference in nucleation density of PS/CbNs decreased with increase in filler content to 0.3 wt%. With increase in foaming temperature, the PS/TRG composite foams showed the highest cell density and no cellular merging and collapsing at 100 and 120 °C. The results obtained from DSC, rheology and positron annihilation lifetime spectroscopy measurements showed that FE nanoparticles exhibited the strongest plasticization effects on the matrix followed by CNTs and TRG nanoparticles. The distinctive plasticization suggested that the shapes of CbNs have a strong influence on the interaction between the PS matrix and the nanoparticles, which could affect the energy barrier for heterogeneous nucleation. The influence of CO2 on the rheology and thermal properties of the composites was investigated using high pressure DSC and high pressure rheology measurements. The results revealed that at high temperature high viscosity became the key factor to determining the cell morphology of nanocomposite foams by preventing bubble coalescence and collapse. © 2018 Society of Chemical Industry
The association between local atherosclerosis of the prostatic artery and benign prostatic enlargement in humans: Putative mechanism of chronic ischemia for prostatic enlargement
Authors: Haga Nobuhiro , Akaihata Hidenori , Hata Junya , Aikawa Ken , Yanagida Tomohiko , Matsuoka Kanako , Koguchi Tomoyuki , Hoshi Seiji , Ogawa Soichiro , Kataoka Masao , Sato Yuichi , Ishibashi Kei , Suzuki Osamu , Hashimoto Yuko , Kojima Yoshiyuki 
Published: May 2018

BackgroundTo investigate the possible pathogenesis of the benign prostatic enlargement (BPE) induced by local atherosclerosis, the association between local atherosclerosis and prostatic enlargement was investigated, and molecular biological analyses were performed using human prostatectomy specimens.MethodsA total of 69 consecutive patients who underwent robot‐assisted radical prostatectomy (RARP) participated in this prospective study. To evaluate actual local atherosclerosis, prostatic arteries were removed during RARP. Microscopic assessment of local atherosclerosis was classified as one of three degrees of narrowing (minimal, moderate, and severe) according to the degree of obstruction of the inner cavity of the prostatic artery. The expressions of several mediators related to chronic ischemia and cell proliferation of the prostate were investigated by immunohistochemistry.ResultsThe median age of the present cohort was 68 (range: 55‐75) years. Although there was no relationship between local atherosclerosis and lower urinary symptoms evaluated by questionnaires, local atherosclerosis was significantly more severe in patients who had a history of treatment for benign prostatic hyperplasia (P = 0.02). Prostate size was significantly larger in the severe local atherosclerosis group than in the minimal and moderate local atherosclerosis groups (P < 0.001 and P = 0.03, respectively). Thepositive expression rates of hypoxia‐inducible factor (HIF)‐1α, malondialdehyde (MDA), transforming growth factor (TGF)‐β1, and basic fibroblast growth factor (bFGF) in the prostate were significantly higher in patients with local atherosclerosis than in patients without local atherosclerosis (all P < 0.01, respectively).ConclusionsIn human surgical specimens, there is evidence that local atherosclerosis of the prostatic artery is significantly associated with prostate size. Given the molecular evidence provided in this study, the putative mechanism for this relationship is that chronic ischemia induced upregulation of oxidative stress pathways, leading to BPE.