bluuebunny/crossref_metadata_2025_split huggingface dataset:
BTXA regulates the epithelial–mesenchymal transition and autophagy of keloid fibroblasts via modulating miR-1587/miR-2392 targeted ZEB2
Authors: Hou Zhanying , Fan Feixiang , Liu Po 
Published: October 2019

Abstract
Keloids are very resistant to treatment in dermatology and plastic surgical practice. The present study aimed to explore the underlying mechanism of botulinum toxin A (BTXA) treated human skin keloid fibroblasts (HSFBs) proving some new insights into keloids treatment. Expression of miR-1587 and miR-2392 were significantly down-regulated in keloid tissues and HSFBs, while the ZEB2 was a target of both and up-regulated in keloid tissues and HSFBs compared with the normal controls. BTXA could significantly increase the expression of miR-1587 and miR-2392 but decrease the expression of ZEB2. BTXA could significantly inhibit the proliferation, cell cycle, and migration and promote apoptosis and autophagy of HSFBs; however, miR-1587 and miR-2392 inhibitors could reverse these effects of BTXA on HSFBs. Silencing ZEB2 could significantly attenuate the effects of miR-1587 and miR-2392 inhibitors in promoting cell proliferation and migration and suppressing apoptosis and autophagy of HSFBs after treating with BTXA. BTXA could suppress the proliferation and migration and promote apoptosis and autophagy of HSFBs via modulating miR-1587/miR-2392 targeted ZEB2.
Role of matrix metalloproteases 1/3 gene polymorphisms in patients with rotator cuff tear
Authors: Miao Kaisong , Jiang Lifeng , Zhou Xindie , Wu Lidong , Huang Yong , Xu Nanwei , Zhang Junjie , Li Jin 
Published: October 2019

Abstract
An association of Matrix Metalloproteinases-1/3 (MMP-1/3) rs1799750/rs3025058 polymorphism with increased risk of rotator cuff tear (RCT) has been reported in a Brazilian population. However, this significant association has not been confirmed in the Chinese population. Genotyping was conducted by polymerase chain reaction (PCR)-restriction fragment length polymorphism and direct sequencing. Our results demonstrated that individuals with the TT genotype had a significantly higher risk of RCT compared with those with the CC genotype. The increased risk of RCT progression was associated with the 2G allele of the rs1799750 polymorphism. No significant association was observed for genotypic and allelic frequencies of the rs3025058 polymorphism. A significant association of the MMP-1 rs1799750 polymorphism was observed with smokers, drinkers and people aged ≥60 years and non-diabetic people. Additionally, the MMP-1 rs1799750 polymorphism was associated with pre-operative stiffness in RCT patients. In conclusion, a significant correlation was identified between the MMP-1 rs1799750 polymorphism and RCT. The MMP-1 rs1799750 polymorphism might be considered as a biomarker of genetically high-risk RCT, helping to clarify the mechanism of RCT.
Role of long non-coding RNA TP73-AS1 in cancer
Authors: Chen Caizhi , Shu Long , Zou Wen 
Published: October 2019

Abstract
Cancer incidence rate has increased so much that it is the second leading cause of deaths worldwide after cardiovascular diseases. Sensitive and specific biomarkers are needed for an early diagnosis of cancer and in-time treatment. Recent studies have found that long non-coding RNAs (lncRNAs) participate in cancer tumorigenesis. LncRNA P73 antisense RNA 1T (TP73-AS1), also known as KIAA0495 and p53-dependent apoptosis modulator (PDAM), is located in human chromosomal band 1p36.32 and plays a crucial role in many different carcinomas. This review summarizes current findings on the role of TP73-AS1 and its signaling pathways in various cancers, including glioma, esophageal squamous cell carcinoma (ESCC), hepatocellular carcinoma (HCC), colorectal cancer (CRC), osteosarcoma, gastric cancer (GC), clear cell renal cell carcinoma (ccRCC), breast cancer (BC), bladder cancer, ovarian cancer, cholangiocarcinoma (CCA), lung cancer, and pancreatic cancer. Its aberrant expression generally correlates with clinicopathological characterization of patients. Moreover, TP73-AS1 regulates proliferation, migration, invasion, apoptosis, and chemoresistance cancer mechanisms, both in vivo and in vitro, through different signaling pathways. Therefore, TP73-AS1 may be considered as a marker for diagnosis and prognosis, also as a target for cancer treatment.
Designing minimalist membrane proteins
Authors: Curnow Paul 
Published: October 2019

Abstract
The construction of artificial membrane proteins from first principles is of fundamental interest and holds considerable promise for new biotechnologies. This review considers the potential advantages of adopting a strictly minimalist approach to the process of membrane protein design. As well as the practical benefits of miniaturisation and simplicity for understanding sequence-structure-function relationships, minimalism should also support the abstract conceptualisation of membrane proteins as modular components for synthetic biology. These ideas are illustrated with selected examples that focus upon α-helical membrane proteins, and which demonstrate how such minimalist membrane proteins might be integrated into living biosystems.
Swimming regulations for protein kinase A catalytic subunit
Authors: Gold Matthew G. 
Published: October 2019

Abstract
cAMP-dependent protein kinase (PKA) plays a central role in important biological processes including synaptic plasticity and sympathetic stimulation of the heart. Elevations of cAMP trigger release of PKA catalytic (C) subunits from PKA holoenzymes, thereby coupling cAMP to protein phosphorylation. Uncontrolled C subunit activity, such as occurs in genetic disorders in which regulatory subunits are depleted, is pathological. Anchoring proteins that associate with PKA regulatory subunits are important for localising PKA activity in cells. However, anchoring does not directly explain how unrestrained ‘free swimming' of C subunits is avoided following C subunit release. In this review, I discuss new mechanisms that have been posited to account for this old problem. One straightforward explanation is that cAMP does not trigger C subunit dissociation but instead activates intact PKA holoenzymes whose activity is restrained through anchoring. A comprehensive comparison of observations for and against cAMP-activation of intact PKA holoenzymes does not lend credence to this mechanism. Recent measurements have revealed that PKA regulatory subunits are expressed at very high concentrations, and in large molar excess relative to C subunits. I discuss the implications of these skewed PKA subunit concentrations, before considering how phosphorylation of type II regulatory subunits and myristylation of C subunits are likely to contribute to controlling C subunit diffusion and recapture in cells. Finally, I speculate on future research directions that may be pursued on the basis of these emerging mechanisms.
Molecular pathways of mitochondrial outer membrane protein degradation
Authors: Zheng Jing , Li Lanlan , Jiang Hui 
Published: October 2019

Abstract
Mitochondrial outer membrane (MOM) encloses inner compartments of mitochondria and integrates cytoplasmic signals to regulate essential mitochondrial processes, such as protein import, dynamics, metabolism, cell death, etc. A substantial understanding of MOM associated proteostatic stresses and quality control pathways has been obtained in recent years. Six MOM associated protein degradation (MAD) pathways center on three AAA ATPases: Cdc48 in the cytoplasm, Msp1 integral to MOM, and Yme1 integral to the inner membrane. These pathways survey MOM proteome from the cytoplasmic and the inter-membrane space (IMS) sides. They detect and degrade MOM proteins with misfolded cytoplasmic and IMS domains, remove mistargeted tail-anchored proteins, and clear mitochondrial precursor proteins clogged in the TOM import complex. These MOM associated protein quality control pathways collaboratively maintain mitochondrial proteostasis and cell viability.
Towards a model of GCN2 activation
Authors: Masson Glenn R. 
Published: October 2019

Abstract
Cells must be able to sense and adapt to their surroundings to thrive in a dynamic environment. Key to adapting to a low nutrient environment is the Integrated Stress Response (ISR), a short-lived pathway that allows cells to either regain cellular homeostasis or facilitate apoptosis during periods of stress. Central to the ISR is the protein kinase General Control Non-depressible 2 (GCN2), which is responsible for sensing starvation. Upon amino acid deficiency, GCN2 is activated and initiates the ISR by phosphorylating the translation initiation factor eIF2α, stalling protein translation, and activating the transcription factor ATF4, which in turn up-regulates autophagy and biosynthesis pathways. A key outstanding question is how GCN2 is activated from an autoinhibited state. Until recently, a model of activation focussed on the increase of deacylated tRNA associated with amino acid starvation, with deacylated tRNA binding directly to GCN2 and releasing autoinhibition. However, in vivo experiments have pointed towards an alternative, deacylated-tRNA-independent mechanism of activation. Here, we review the various factors that may facilitate GCN2 activation, including recent research showing that the P-stalk complex, a ribosome-associated heteropentameric protein complex, is a potent activator of GCN2.
Immunadsorption und therapeutischer Plasmaaustausch
Authors: Tölle Markus 
Published: October 2019

ZUSAMMENFASSUNGDie zur Verfügung stehenden Aphereseformen sind wirksame und komplementäre Therapieoptionen u. a. für die Behandlung unterschiedlicher Autoimmunerkrankungen. Neben dem therapeutischen Plasmaaustausch bedeutet die Immunadsorption einen wichtigen Fortschritt unserer Behandlungsmöglichkeiten. In vielen Bereichen der Medizin, insbesondere in der Nephrologie und der Neurologie, konnten autoimmunologische Mechanismen als Auslöser zahlreicher Erkrankungen identifiziert werden. Bei vielen dieser Krankheitsbilder stellt die therapeutische Apherese eine wertvolle Therapieoption dar, insbesondere bei ausgeprägter klinischer Symptomatik oder bei therapieresistenten Verläufen. Im Rahmen dieses Artikels wird auf den Einsatz der therapeutischen Apherese bei den wichtigsten nephrologischen und neurologischen Erkrankungen eingegangen.
Arthroscopic-Guided Balloon Tibioplasty in Schatzker III Tibial Plateau Fracture
Authors: Cuzzocrea Fabrizio , Jannelli Eugenio , Ivone Alessandro , Perelli Simone , Fioruzzi Alberto , Ghiara Matteo , Zanon Giacomo , Benazzo Francesco 
Published: October 2019

Abstract
Purpose The study aims to present the results at a mean 28-months follow-up of arthroscopic-guided balloon tibioplasty and to spot some technical tricks and some practice using tools and materials.
Methods The study relates to six patients with tibial plateau fractures type Schatzker III with tibial plateau depression more than 4 mm at preoperative computed tomography scan (CT-scan). The follow-up period ranged from 22 to 33 months, with a mean of 28 months. No patients were lost to follow-up. The patients were evaluated clinically using the Rasmussen score system and Lysholm score systems at 6 to 12 and 24 months, postoperatively. Radiographic evaluations (standard X-rays) were done preoperatively at 1, 3, and 12 months postoperatively while a CT-scan with 3D reconstruction was performed preoperatively, at the first day and 6 months, postoperatively.
Results The mean Rasmussen clinical score at 6 months postoperatively was 26.3 while at 1-year postoperatively the mean Rasmussen clinical score was 28.33. At 2-year postoperatively the mean Rasmussen clinical score was 28.83. Statistically significant difference was found in 6-months and 2-years results (p < 0.05). CT-scan achieved the first postoperative day showed the recovery of approximately 70% of the area of the interested tibial plateau, restoring of the joint surface without articular bone free fragments.
Conclusion The described surgical procedure, if correctly performed with proper indications (Schatzker III), respect the principles mentioned above and the clinical and radiological results confirm our purpose.
Level of Evidence This is a therapeutic case series, level IV study.
The Same is Not the Same: Device Effect during Bipolar Radiofrequency Ablation of Atrial Fibrillation
Authors: Niemann Bernd , Dominik Elisabeth , Rohrbach Susanne , Grieshaber Philippe , Roth Peter , Böning Andreas 
Published: October 2019

Abstract
Background Different ablation devices deliver the same type of energy but use individual control mechanisms to estimate efficacy. We compared patient outcome after the application of radiofrequency ablation systems, using temperature- or resistance-control in paroxysmal and persistent atrial fibrillation (AF).
Methods This is an unselected all-comers study. Patients underwent standardized left atrial (paroxysmal atrial fibrillation, [PAF] n = 31) or biatrial ablation (persistent atrial fibrillation [persAF] n = 61) with bipolar RF from October 2010 to June 2013. Patients with left atrial dilatation (up to 57 mm), reduced left ventricular (LV) function, and elderly were included. We used resistance-controlled (RC) or temperature-controlled (TC) devices. We amputated atrial appendices and checked intraoperatively for completeness of pulmonary vein exit block. All patients received implantable loop recorders. Follow-up interval was every 6 months. Antiarrhythmic medical treatment endured up to month 6.
Results We reached 100% freedom from atrial fibrillation (FAF) in PAF. In perAF 19% of the RC but 82% of the TC patients reached FAF (12 months; p < 0.05). TC patients exhibited higher creatine kinase-muscle/brain (CK-MB) peak values. In persAF, CK-MB-levels correlated to FAF. No and no mortality (30 days) was evident. Twelve-month mortality did not correlate to AF type, AF duration, LV dimension, or function and age. Prolonged need of oral anticoagulants was 90.1% (RC) and 4.5% (TC).
Conclusion In patients with persAF undergoing RF ablation, TC reached higher FAF than RC. Medical devices are not “the same” regarding effectiveness even if used according to manufacturer's instructions. Thus, putative application of “the same” energy is not always “the same” efficacy.
Negative Concord and sentential negation in Gallo
Authors: Guilliot Nicolas , Becerra-Zita Samantha 
Published: October 2019

Abstract
The goal of this paper is to get a better
understanding of Negative Concord (NC) in natural language through
the study of Gallo, a Romance language spoken in Brittany. Despite
obvious similarities with respect to Standard French (SF), Gallo
differs from it by integrating sentential negation
pâ/pouin ‘not’ to the NC system. We will first
show that previous analyses of the phenomenon based on specific
properties of n-words (see Déprez (2003, 2016), Zeijlstra (2004, 2010)) fail to account for the difference between Gallo
and SF. Then we will argue for an alternative approach (inspired by
Muller (2010) and
Homer (2013)) based
on properties of sentential negation itself. Finally, we will
propose how to account for inter/intra-individual microvariation of
NC in Gallo.
Point of view on causal clauses
Authors: Charnavel Isabelle 
Published: October 2019

Abstract
This paper uses the case study of French clauses
introduced by parce que ‘because’ and
puisque ‘since’ to argue that causal clauses
are intrinsically perspectival: the causal relation that they
express is established by a causal judge. Perspectival effects in
causal clauses indicate that the referential possibilities of this
judge depend on the structural level of attachment of causal
clauses, which can modify Verb Phrases, Evidential Phrases or Speech
Act Phrases. This supports the hypothesis that the causal judge is
syntactically represented as a silent argument of the causal
subordinator that must be bound within its clause. The presence of
this judge argument explains why logophoric elements can appear in
causal clauses.
From a Romance null subject grammar to a non-null subject
grammar
Authors: Teixeira Joana 
Published: October 2019

Abstract
This study investigates the acquisition of
pronominal subjects by advanced and near-native speakers of English
whose L1s are European Portuguese (EP), a null subject language
(NSL), and French, a non-NSL (NNSL). Two experimental tasks were
used: a drag-and-drop task and a speeded acceptability judgement
task. Results show that the EP speakers who have an advanced level
of English fail to reject expletive and [ animate] null subjects in
the speeded task. In contrast, those who are at a near-native level
behave native-like across all tasks, just as French speakers do.
These findings indicate that the syntax of subjects is acquirable in
L1 NSL-L2 NNSL pairings, despite giving rise to significant
developmental delays. Developmental problems are argued to result
from input misanalysis.
Color terms in Basque
Authors: Ibarretxe-Antuñano Iraide 
Published: October 2019

This chapter explores the domain of color terms in Basque
from a descriptive, as well as a categorization perspective. The
first part provides an overview of the color system in this
language: its color lexicalization processes and the meanings these
color labels cover. The second part focuses on the results of a
categorization study of color in Basque. The data were retrieved
from 20 native speakers of Basque and elicited using the color
elicitation task as part of the EoSS project at the Max Planck
Institute for Psycholinguistics (Majid et al., 2015). The chapter concludes with a
discussion on the similarities and differences found in the
descriptive and the categorization part.
Temporal reference in Vietnamese
Authors: Bui Thuy 
Published: October 2019

Abstract
The paper seeks to address gaps in current knowledge regarding cross-linguistic variation on the semantics of tense and aspect by examining how temporal reference is determined in Vietnamese. It is argued that, like St’át’imcets (Matthewson 2006), Vietnamese is a ‘superficially tenseless’ language in the sense that even though the grammar lacks overt tense morphology, every finite clause contains a phonologically empty non-future tense morpheme. I then argue that future tense in Vietnamese realised as a combination both the obligatory null tense morpheme and an overt spell-out of sẽ, which is the Vietnamese counterpart of the English WOLL operator (Abusch 1985). This means that sẽ itself is neither a purely a tense or solely an epistemic modal. Consequently, this study concludes that future time reference is systematically different from present and past time interpretations, which is similar to previous claims made by Abusch (1997), Kratzer (1998), and others.
(In)definiteness of Vietnamese noun phrases
Authors: Đoàn Quý Ngọc Thị , Everaert Martin , Reuland Eric 
Published: October 2019

Abstract
In this contribution we discuss the factors determining the definite or indefinite interpretation of Noun Phrases in Vietnamese. In contrast to what has been claimed in the literature, we find that NPs consisting of a bare noun and NPs consisting of a numeral, a classifier (cl) and a noun, while generally indefinite, may permit a definite interpretation in certain contexts (in contrast to NPs consisting of just a classifier and a noun, which are always definite). We then compare the relevant patterns in Vietnamese with their counterparts in Mandarin and Cantonese. Although the differences may seem striking we show that they can be captured by some simple morpho-syntactic parameters.
Traditional knowledge systems and the role of traditional medicine in Jamaica.
Authors: Picking D. , Delgoda R. , Vandebroek I. 
Published: October 2019

Abstract
Jamaica's national motto 'Out of Many, One People' reflects the country's multiracial past and the historic and ongoing influences that many cultures have had on the country's traditional knowledge systems (TKS) and traditional medicine (TM). This review explores Jamaican TKS and TM, from the earliest indigenous peoples to their use today. Historically, a significant proportion of the Jamaican population has relied on different forms of TM, and today 73% of Jamaicans self-medicate with plant-based medicines on a regular basis. Modern Jamaica effectively has a spectrum of healthcare provision operating between two healthcare systems, western biomedicine and TM, although the latter is often dismissed as lacking western scientific rigour. Crucial to the development of TKS and TM has been the island's abundant flora, including endemic, native and introduced plants, giving rise to a uniquely Jamaican vegetation. Following a declining oral tradition of transmission, Jamaican TKS and TM also face a concurrent loss of biological diversity that forms an indispensable constituent of these systems, making their conservation and protection a priority. Jamaica is poised to develop a natural health products' industry with the potential to contribute to national growth and development. However, for such development to flourish on an equitable basis across Jamaican society requires recognition of TKS, and legislation that protects TKS and the holders of TKS and guarantees fair and equitable sharing of benefits derived from the commercial use of these knowledge systems. At a time when the rights of indigenous peoples internationally are increasingly recognized, it is also important for Afro-descendant communities in the Caribbean and Latin America to achieve better recognition and to be incorporated into the dialogue on traditional, local and indigenous knowledge systems and their contributions to the United Nations Sustainable Development Goals.
Larvae of
Cephalopina titillator
(Clark, 1816) (Diptera: Oestridae) causing nasopharyngeal myiasis of camels: an overview.
Authors: Basu A. K. , Charles R. A. 
Published: October 2019

Abstract

In this review article, the authors present an overview on nasopharyngeal myiasis due to the larvae of
Cephalopina titillator
syn.
Cephdopsis titillator
which is found in the nasopharynx of camels, especially in tropical and sub-tropical regions in Africa and Asia. Among the parasitic diseases of camel, the larvae of
C. titillator
adversely affect the health of these dromedaries and cause significant economic losses in many camel-producing countries. The prevalence, geographical distribution and life-cycle of the parasite, clinical signs, pathology and treatment due to this parasitic infection have been discussed in this review.
Transfer printing of nanomaterials and microstructures using a wire bonder
Authors: Wang Xiaojing , Schröder Stephan , Enrico Alessandro , Kataria Satender , Lemme Max C , Niklaus Frank , Stemme Göran , Roxhed Niclas 
Published: October 2019

Abstract
Scalable and cost-efficient transfer of nanomaterials and microstructures from their original fabrication substrate to a new host substrate is a key challenge for realizing heterogeneously integrated functional systems, such as sensors, photonics, and electronics. Here we demonstrate a high-throughput and versatile integration method utilizing conventional wire bonding tools to transfer-print carbon nanotubes (CNTs) and silicon microstructures. Standard ball stitch wire bonding cycles were used as scalable and high-speed pick-and-place operations to realize the material transfer. Our experimental results demonstrated successful transfer printing of single-walled CNTs (100

m-diameter patches) from their growth substrate to polydimethylsiloxane, parylene, or Au/parylene electrode substrates, and realization of field emission cathodes made of CNTs on a silicon substrate. Field emission measurements manifested excellent emission performance of the CNT electrodes. Further, we demonstrated the utility of a high-speed wire bonder for transfer printing of silicon microstructures (60

m

60

m

20

m) from the original silicon on insulator substrate to a new host substrate. The achieved placement accuracy of the CNT patches and silicon microstructures on the target substrates were within

4

m. These results show the potential of using established and extremely cost-efficient semiconductor wire bonding infrastructure for transfer printing of nanomaterials and microstructures to realize integrated microsystems and flexible electronics.