bluuebunny/crossref_metadata_2025_split huggingface dataset:
Allelic Imbalance of Recurrently Mutated Genes in Acute Myeloid Leukaemia
Authors: Batcha Aarif M. N. , Bamopoulos Stefanos A. , Kerbs Paul , Kumar Ashwini , Jurinovic Vindi , Rothenberg-Thurley Maja , Ksienzyk Bianka , Philippou-Massier Julia , Krebs Stefan , Blum Helmut , Schneider Stephanie , Konstandin Nikola , Bohlander Stefan K. , Heckman Caroline , Kontro Mika , Hiddemann Wolfgang , Spiekermann Karsten , Braess Jan , Metzeler Klaus H. , Greif Philipp A. , Mansmann Ulrich , Herold Tobias 
Published: August 2019

AbstractThe patho-mechanism of somatic driver mutations in cancer usually involves transcription, but the proportion of mutations and wild-type alleles transcribed from DNA to RNA is largely unknown. We systematically compared the variant allele frequencies of recurrently mutated genes in DNA and RNA sequencing data of 246 acute myeloid leukaemia (AML) patients. We observed that 95% of all detected variants were transcribed while the rest were not detectable in RNA sequencing with a minimum read-depth cut-off (10x). Our analysis focusing on 11 genes harbouring recurring mutations demonstrated allelic imbalance (AI) in most patients.GATA2,RUNX1,TET2,SRSF2,IDH2,PTPN11,WT1,NPM1andCEBPAshowed significant AIs. While the effect size was small in general,GATA2exhibited the largest allelic imbalance. By pooling heterogeneous data from three independent AML cohorts with paired DNA and RNA sequencing (N = 253), we could validate the preferential transcription ofGATA2-mutated alleles. Differential expression analysis of the genes with significant AI showed no significant differential gene and isoform expression for the mutated genes, between mutated and wild-type patients. In conclusion, our analyses identified AI in nine out of eleven recurrently mutated genes. AI might be a common phenomenon in AML which potentially contributes to leukaemogenesis.
Correlation of periodontal and microbiological evaluations, with serum levels of estradiol and progesterone, during different trimesters of gestation
Authors: Massoni Renata Santos de Souza , Aranha Andreza Maria Fábio , Matos Fernanda Zanol , Guedes Orlando Aguirre , Borges Álvaro Henrique , Miotto Monize , Porto Alessandra Nogueira 
Published: August 2019

AbstractOur purpouse was to identify quantitatively and qualitatively the subgingival flora in different gestational trimesters, compared to non-pregnant women; evaluating the correlations between epidemiological characteristics, clinical diagnosis, microbiological findings and levels of estradiol and progesterone. 52 pregnant women divided into 3 groups, according to the gestational trimester and 15 non-pregnant patients, without hormonal contraceptives, were evaluated. Plaque index (PI), gingival index (GI), probing depth (PD) and clinical attachment level (CAL) were evaluated. Subgingival biofilm samples were processed by the qPCR technique and the serum levels of estradiol and progesterone quantified by chemiluminescence. Clinical diagnosis during gestation was correlated with the total bacterial count. A higher prevalence of Tannerella forsythia (Tf) was identified in first trimester of pregnancy and this periodontopathogen was correlated with the diagnosis of gingivitis among pregnant women. Porphyromonas gingivalis (Pg) showed a positive correlation with progesterone levels in the first trimester. High prevalence of periodontopathogens was noticed in this population. Clinical diagnosis in gestation was positively correlated with the total amount of bacteria, without influence of the hormonal levels or the epidemiological factors evaluated. The presence of Tf favored occurrence of gingivitis during pregnancy and the progesterone levels in the first trimester enhanced the growth of Pg.
Risk factors and clinical features for post-transplant thoracic air-leak syndrome in adult patients receiving allogeneic haematopoietic stem cell transplantation
Authors: Liu Yao-Chung , Chou Yi-Hsin , Ko Po-Shen , Wang Hao-Yuan , Fan Nai-Wen , Liu Chia-Jen , Hsiao Liang-Tsai , Chien Sheng-Hsuan , Chiou Tzeon-Jye , Liu Jin-Hwang , Gau Jyh-Pyng 
Published: August 2019

AbstractPost-transplant thoracic air-leak syndrome (ALS) is rare but potentially life-threatening in patients receiving allogeneic haematopoietic stem cell transplantation (HSCT). Nevertheless, papers on thoracic ALS are limited, and this complication remains largely unknown. We reviewed 423 adult patients undergoing allogeneic HSCT from 2003 to 2014. Risk factors, clinical features and survival for thoracic ALS were collected and analysed. Thirteen out of 423 patients (3.1%) developed post-transplant thoracic ALS, including two ALS patients in the early phase. The median age at HSCT was 33 years among 13 patients with thoracic ALS. Male patients were predominant (69%). The median onset time was 253 days (range: 40–2680) after HSCT. Multivariate analysis revealed that grade III–IV acute graft-versus-host disease (GVHD) (p = 0.017), extensive chronic GVHD (cGVHD) (p = 0.019) and prior history of pulmonary invasive fungal infection (p = 0.007) were significant risk factors for thoracic ALS. In patients with cGVHD, those with thoracic ALS had a significantly worse survival than those without thoracic ALS (p = 0.04). Currently, published data analysing and exploring post-transplant thoracic ALS are limited. Our study employed a large patient cohort and determined the risk factors and clinical features for post-transplant thoracic ALS.
Impact of maternal characteristics on human milk oligosaccharide composition over the first 4 months of lactation in a cohort of healthy European mothers
Authors: Samuel Tinu Mary , Binia Aristea , de Castro Carlos Antonio , Thakkar Sagar K. , Billeaud Claude , Agosti Massimo , Al-Jashi Isam , Costeira Maria Jose , Marchini Giovanna , Martínez-Costa Cecilia , Picaud Jean-Charles , Stiris Tom , Stoicescu Silvia-Maria , Vanpeé Mireille , Domellöf Magnus , Austin Sean , Sprenger Norbert 
Published: August 2019

AbstractHuman milk oligosaccharide (HMO) composition varies among lactating mothers and changes during the course of lactation period. Interindividual variation is largely driven by fucosyltransferase (FUT2 and FUT3) polymorphisms resulting in 4 distinct milk groups. Little is known regarding whether maternal physiological status contributes to HMO variability. We characterized the trajectories of 20 major HMOs and explored whether maternal pre-pregnancy body mass index (ppBMI), mode of delivery, or parity may affect milk HMO composition. Using longitudinal breastmilk samples from healthy mothers (n = 290) across 7 European countries, we characterized HMO composion and employed mixed linear models to explore associations of maternal characteristics with individual HMOs. We observed HMO-specific temporal trajectories and milk group dependencies. We observed relatively small but significant differences in HMO concentrations based on maternal ppBMI, mode of delivery and parity. Our findings suggest that HMO composition to be regulated time-dependently by an enzyme as well as substrate availability and that ppBMI, mode of delivery, and parity may influence maternal physiology to affect glycosylation marginally within the initital period of lactation. Our observational study is the largest European standardized and longitudinal (up to 4 months) milk collection study assessing HMO concentrations and basic maternal characteristics. Time of lactation and milk groups had the biggest impact on HMO variation. Future studies need to elucidate these observations and assess the physiological significance for the breastfed infant.
Point-of-need and Portable Miniaturized Devices for Forensic Chemical Sensing
Authors: de Araujo William Reis 
Published: August 2019

This book chapter briefly describes the relevance and main contribution of portable and miniaturized methods for forensic scenario, highlighting various types of detectors and platforms commonly used. From traditional equipment employed for the identification of (bio)chemical trace evidence such as mass, Raman, and NIR spectrometers, whose instrumental advances enabled their miniaturization and portability; to conventionally portable and low-cost devices such as colorimetric, electrochemical, microchip and paper-based analytical devices. A wide range of forensic applications is presented, critically discussed and some remarkable considerations for future studies are pointed out.
MicroRNA-302c enhances the chemosensitivity of human glioma cells to temozolomide by suppressing P-gp expression
Authors: Wu YiHan , Yao Yuan , Yun YongLi , Wang MeiLing , Zhu RunXiu 
Published: August 2019

Abstract
Increasing evidence indicates that microRNAs (miRNAs) participate in the regulation of chemoresistance in a variety of cancers including glioma. However, the molecular mechanism underlying the development of chemoresistance in glioma is not well understood. The aim of the present study was to explore the role of miRNAs in the chemosensitivity of glioma cells and the underlying mechanism. By microarray and qRT-PCR, we observed significant down-regulation of microRNA-302c (miR-302c) in the temozolomide (TMZ)-resistant human glioma tissues/cells. The low expression of miR-302c was closely associated with poor prognosis and chemotherapy resistant in patients. miR-302c up-regulation re-sensitized U251MG-TMZ cells and LN229-TMZ cells to TMZ treatment, as evidenced by inhibition of the cell viability, cell migration, and invasion capacity, and promotion of the apoptosis after TMZ treatment. Furthermore, P-glycoprotein (P-gp) was identified as a functional target of miR-302c and this was validated using a luciferase reporter assay. In addition, P-gp was found to be highly expressed in U251MG-TMZ cells and there was an inverse correlation between P-gp and miR-302c expression levels in clinical glioma specimens. Most importantly, we further confirmed that overexpression of P-gp reversed the enhanced TMZ-sensitivity induced by miR-302c overexpression in U251MG-TMZ and LN229-TMZ cells. Our finding showed that up-regulation of miR-302c enhanced TMZ-sensitivity by targeting P-gp in TMZ-resistant human glioma cells, which suggests that miR-302c would be potential therapeutic targets for chemotherapy-resistant glioma patients.
miR-497-5p inhibits gastric cancer cell proliferation and growth through targeting PDK3
Authors: Feng Lan , Cheng Kai , Zang Rongjia , Wang Qingdong , Wang Jianjie 
Published: August 2019

Abstract
MicroRNA plays an important role in gastric cancer (GC) development, while the function of miR-497-5p in this disease remains unknown. In the present study, we demonstrated miR-497-5p as a tumor suppressive microRNA in GC. miR-497-5p was down-regulated in GC tissues and its expression was associated with the disease stage. Inhibition of miR-497-5p promoted GC cell proliferation and growth. By contrast, miR-497-5p ectopic expression suppressed the proliferation and growth of GC cells. In addition, miR-497-5p inhibited DNA synthesis and enhanced apoptosis in GC cells. The cell cycle progression was suppressed by miR-497-5p. Mechanistically, miR-497-5p directly targeted and suppressed the expression of pyruvate dehydrogenase kinase 3 (PDK3), which is highly expressed in GC tissues. Over-expression of PDK3 promoted the proliferation of GC cells. Our study revealed that miR-497-5p inhibited GC cell proliferation and growth via targeting PDK3.
Indoor environmental quality evaluation in NZEB
Authors: Sánka Imrich , Schoberer Thomas , Stutterecker Werner , Petráš Dušan 
Published: August 2019

The European Commission in 2010 accepted Energy Performance of Buildings Directive (EPBD) and the 2012 Energy Efficiency Directive (EED) are the main energy conservation legislative instruments for to reduce the energy consumption of new built and renovated buildings in Europe. The national regulation based on EPBD states that after the year 2016 only so called ultra-low energy buildings can be built. The next tightening in energy saving will come after 2021 (for commercial buildings after 2019), when only nearly zero energy buildings (NZEB) would be allowed to build. It means that these buildings must fulfil A0 category requirements by energy labelling.
But what about the indoor environmental quality in objects like this? This article shows results of indoor environmental quality measurement in NZEB building. Indoor air temperature, relative humidity, carbon dioxide concentration and air exchange rate had been measured. Except these parameters energy consumption from the grid and from the photovoltaic panel had been evaluated.
Energy and economical evaluation of residential buildings in Slovakia
Authors: Bartošová Kmeťková Jana , Petráš Dušan 
Published: August 2019

The main objective of the research is to make evaluation of energy and economic by the retrofitting of the residential buildings. If this methodology can be an appropriate tool to guide decisions related with the building energy performances and to identify the most cost-effective variants of the renovation, that could be applied to the building stock in Slovakia by the analysis of the life-cycle costs of the representative apartment buildings.
The specific objectives of this study were the following:
• Theoretical analysis of the residential buildings stock in Slovakia
• Analysis of the studied residential buildings and their energy parameters before and after renovation
• Simulation of the energy performance of the apartment buildings
• Mathematical modeling of the technical and economic parameters
Data-driven study on individual occupant comfort using heating setpoints and window openings in new low-energy apartments – preliminary insights
Authors: Sarran Lucile , Christensen Morten Herget , Hviid Christian Anker , Radoszynski Andrea Marin , Rode Carsten , Pinson Pierre 
Published: August 2019

This work suggests a method to evaluate residential building occupants’ neutral temperature in winter based on their interaction with their heating system.
This study applies the developed method on eight new, low-energy apartments in Copenhagen, Denmark. A set of indoor temperature, heating setpoint, window opening and floor heating valve opening data was collected from mid-January to the end of April, spanning through a large part of the Danish heating season. Semi-structured interviews were performed with occupants of three of the eight apartments in order to understand their use of their heating system.
This preliminary study permits to highlight the potential and the current limitations of the proposed method, both for neutral temperature estimation as such and for applications in optimizing the energy flexibility provided by the building. This article suggests directions for further elaboration of the model. The main two influential factors highlighted here affecting setpoint adjustment are the occupants’ acceptability of temperature variation and their ability to control the heating system.
Two software tools for facilitating the choice of ground source heat pumps by stakeholders and designers
Authors: De Carli Michele , Aguirre Amaia Castelruiz , Zarrella Angelo , Cardoso Lucia , Noyé Sarah , Gast Robert , Graci Samantha , Emmi Giuseppe , Bertermann David , Müller Johannes , Galgaro Antonio , Santa Giorgia Dalla , Poletto Fabio , Mezzasalma Giulia , Contini Silvia , Urchueguía Javier , Marco Belliardi , Pasquali Riccardo , Bernardi Adriana 
Published: August 2019

For promoting the diffusion of GSHP and making the technology more accessible to the general public, in the H2020 research project “CHeap and Efficient APplication of reliable Ground Source Heat exchangers and PumpS” (acronym Cheap-GSHPs) a tool for sizing these systems has been developed, as well as a Decision Support System (DSS) able to assist the user in the preliminary design of the most suitable configuration.
For all these tools a common platform has been carried out considering climatic conditions, energy demand of buildings, ground thermal properties, heat pump solutions repository, as well as renewable energy database to use in synergy with the GSHPs. Since the aims of the tools are different, there are different approaches.
The design tool is mainly addressed to designers. The calculation may be done in two ways: with a simplified method based on the ASHRAE approach and with a detailed calculation based on the numerical tool CaRM (Capacity-Resistance method).
The DSS final aim is to support decision-making, by providing the stakeholders at all the level with a series of scenario. The Cheap-GSHPs project has developed a DSS tool aimed at accelerating the decision-making process of designers and building owners as well as increasing market share of the Cheap-GSHPs technologies. Hence the DSS generates different possible solutions based on a defined general problem, identifying the optimal solution.
Both tools are presented in the paper, showing the potentialities provided by both software.
Der gynäkologische Notfall – Teil 1: Präsentationssymptom unklares Abdomen
Authors: Weißleder Anne , Egbe Anne , Beinkofer Daniela , Kulla Martin , Treffer Dominik 
Published: August 2019

ZusammenfassungDer Artikel stellt das typische Präsentationssymptom des gynäkologischen Notfalls – das unklare Abdomen der Frau – anhand von Szenarien aus dem prä- und innerklinischen Bereich vor. Die genaue Notfallanamnese und -untersuchung werden für die gynäkologische Patientin modifiziert. Wichtige Differenzialdiagnosen des unklaren Abdomens sowie weitere gynäkologische Notfallszenarien, wie z.B. genitales Trauma, werden in den kommenden Ausgaben erläutert.
Systematic Review and Meta-Analysis of Randomized Controlled Trials on the Effect of SGLT2 Inhibitor on Blood Leptin and Adiponectin Level in Patients with Type 2 Diabetes
Authors: Wu Peili , Wen Weiheng , Li Jitong , Xu Jie , Zhao Min , Chen Hong , Sun Jia 
Published: August 2019

AbstractSodium glucose cotransporter 2 (SGLT2) inhibitors are a new kind of hypoglycemic drugs that improve glucose homeostasis by inhibiting renal glucose reabsorption. Recent studies have shown that SGLT2 inhibitors can also mediate body metabolism through regulation of adipokines level, but the effects of SGLT2 inhibitors on the concentration of adipokines (leptin and adiponectin) remains controversial. This meta-analysis was set out to evaluate the changes in circulating leptin and adiponectin levels in patients with type 2 diabetes mellitus (T2DM) receiving SGLT2 inhibitors therapy. Ten randomized controlled trials (RCTs), that evaluated the effects of SGLT2 inhibitors on blood leptin and adiponectin levels in patients with type 2 diabetes, were identified by performing a systematic search of Pubmed, Embase, Cochrane, and Web of science databases through July 2018. Data were calculated using a random-effects model and presented as standardized mean difference (SMD) and 95% conﬁdence interval (CI). Compared with placebo, treatment with SGLT2 inhibitors contributed to a decreased circulating leptin levels (SMD −0.29, 95% CI −0.56, −0.03) and an increased circulating adiponectin levels (SMD 0.30, 95% CI 0.22, 0.38). SGLT2 inhibitor treatment was associated with decreased circulating leptin levels and increased circulating adiponectin levels, which might contribute to the beneficial effects of SGLT2 inhibitors on metabolic homeostasis.
Medizinische Rehabilitation bei MS: Barrieren und Facilitatoren der
Inanspruchnahme aus Patientensicht
Authors: Giesler Jürgen M. , Klindtworth Katharina , Nebe Angelika , Glattacker Manuela 
Published: August 2019

Zusammenfassung
Ziel der Studie Leistungen der stationären medizinischen
Rehabilitation werden von MS-Erkrankten vergleichsweise selten genutzt,
obwohl Belege für deren Wirksamkeit vorliegen. Ziel der vorliegenden
Studie war es deshalb, von den Betroffenen wahrgenommene Barrieren und
Facilitatoren der Inanspruchnahme stationärer Rehabilitationen zu
ermitteln.
Methodik Auf der Basis umfangreicher Vorarbeiten wurde ein Fragebogen
mit 45 Items entwickelt, der bundesweit von 590 MS-Erkrankten mehrheitlich
online beantwortet wurde. Die Auswertung erfolgte mittels deskriptiver
statistischer Verfahren.
Ergebnisse Mehrheitlich hohe Erwartungen der Befragten an die
MS-Rehabilitation betreffen v.a. Aspekte der Strukturqualität der
Einrichtungen und die Mitwirkung an Behandlungsentscheidungen. Als Barrieren
einer Inanspruchnahme werden zu geringe Kenntnisse der Inhalte und
Abläufe der medizinischen Rehabilitation genannt und eine z.T. als
zu gering erlebte Unterstützung durch Ärzte in Bezug auf das
Stellen eines Antrags auf Rehabilitation. Als weitere Barrieren werden eine
als gering erlebte Transparenz der Bewilligungsprozesse und – von
einem kleineren Anteil der Befragten – auch berufs- oder
familienbezogene Aspekte gesehen.
Schlussfolgerungen Auf Basis der Befunde lassen sich verschiedene
Empfehlungen formulieren, die zu einem Abbau von Barrieren der
Inanspruchnahme einer medizinischen Rehabilitation von MS-Erkrankten
beitragen können. Diese beinhalten Hinweise auf bzw. die
Bereitstellung von im Einzelfall abzustimmender Information über die
medizinische Rehabilitation bei MS (Ziele, Nutzen und Inhalte), die
Erhöhung der Transparenz des Antrags- und Genehmigungsprozesses
sowie die Stärkung der Rolle ambulanter Fachärzte.
Profiles of Phenolic Acids and Triterpene Glycosides in Commercial and Cultivated Black Cohosh
Authors: Bittner Marian , Schenk Regina , Springer Andreas , Melzig Matthias F. 
Published: August 2019

AbstractThe medicinal plant Actaea racemosa is a perennial, whose rhizome (black cohosh rhizome) is usually wild harvested on a multiton scale to meet market requirements. Since this North American species is increasingly endangered, cultivation is needed. Even though studies prove that cultivation is possible, it has not been widely established. This may be due to a different quality of cultivated material, which does not comply with current pharmacopoeial requirements. This study compares contents and chromatographic fingerprints of phenolic acids and triterpene glycosides in different types of black cohosh rhizomes. Commercial batches from wild harvests were compared to individual plants from the wild and from cultivation. Phenolic acidsʼ contents and profiles were generally comparable between wild harvesting and cultivation. On the contrary, the total triterpene glycoside content was significantly lower in cultivation (p ≤ 0.001). In individual plants, different profiles of triterpene glycosides occurred. Possibly, specimen or chemotype selection for cultivation would cause a shift of the triterpene glycoside profile of cultivation batches away from the common pattern found in batches from wild harvesting. Potentially, such differences have an impact on the efficacy of black cohosh herbal products, if cultivated plant material is used for manufacturing.
Nitric Oxide and Opioids Involvement in Isobolographic Nsaids Antinociception
Authors: Miranda Hugo F. , Noriega Viviana , Sierralta Fernando , Poblete Paula , Aranda Nicolás , Prieto Juan Carlos 
Published: August 2019

AbstractDifferent NSAIDs are used as antinociceptive in various analgesic assays, among which should be mentioned: ibuprofen, ketorolac , ketoprofen, meloxicam , paracetamol and others. It has been shown that NSAIDs possess antinociceptive activity by blocking cyclooxygenase enzymes (COXs). The present study was designed to evaluate the possible involvement of the nitridergic pathway due to L-NAME and the opioidergic route by NTX in the antinoception induced by NSAIDs using a murine pain model the tail flick test in an automatic tail flick algesiometer. The antinociception was evaluated by means of isobolographic analysis. The interaction between the combination of NSAIDs, via i.p., on basis of their ED25, demonstrated that the coadministration of the drugs were synergistic with the exception of the lack of effect in combination of meloxicam with ibuprofen and with ketorolac, since the result was additive. These data validate that the NSAIDs administered alone or in combinations produce antinociception in which other mechanisms of action must be added to the simple inhibition of COXs. In addition, the pretreatment of the mice with L-NAME and NTX does not change previous isobolographic parameters of the mixture of NSAIDs.