bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanoprinting organic molecules at the quantum level
Authors: Hail Claudio U. , Höller Christian , Matsuzaki Korenobu , Rohner Patrik , Renger Jan , Sandoghdar Vahid , Poulikakos Dimos , Eghlidi Hadi 
Published: April 2019

AbstractOrganic compounds present a powerful platform for nanotechnological applications. In particular, molecules suitable for optical functionalities such as single photon generation and energy transfer have great promise for complex nanophotonic circuitry due to their large variety of spectral properties, efficient absorption and emission, and ease of synthesis. Optimal integration, however, calls for control over position and orientation of individual molecules. While various methods have been explored for reaching this regime in the past, none satisfies requirements necessary for practical applications. Here, we present direct non-contact electrohydrodynamic nanoprinting of a countable number of photostable and oriented molecules in a nanocrystal host with subwavelength positioning accuracy. We demonstrate the power of our approach by writing arbitrary patterns and controlled coupling of single molecules to the near field of optical nanostructures. Placement precision, high yield and fabrication facility of our method open many doors for the realization of novel nanophotonic devices.
SIRT3 mediates hippocampal synaptic adaptations to intermittent fasting and ameliorates deficits in APP mutant mice
Authors: Liu Yong , Cheng Aiwu , Li Yu-Jiao , Yang Ying , Kishimoto Yuki , Zhang Shi , Wang Yue , Wan Ruiqian , Raefsky Sophia M. , Lu Daoyuan , Saito Takashi , Saido Takaomi , Zhu Jian , Wu Long-Jun , Mattson Mark P. 
Published: April 2019

AbstractIntermittent food deprivation (fasting, IF) improves mood and cognition and protects neurons against excitotoxic degeneration in animal models of epilepsy and Alzheimer’s disease (AD). The mechanisms by which neuronal networks adapt to IF and how such adaptations impact neuropathological processes are unknown. We show that hippocampal neuronal networks adapt to IF by enhancing GABAergic tone, which is associated with reduced anxiety-like behaviors and improved hippocampus-dependent memory. These neuronal network and behavioral adaptations require the mitochondrial protein deacetylase SIRT3 as they are abolished in SIRT3-deficient mice and wild type mice in which SIRT3 is selectively depleted from hippocampal neurons. In the AppNL-G-F mouse model of AD, IF reduces neuronal network hyperexcitability and ameliorates deficits in hippocampal synaptic plasticity in a SIRT3-dependent manner. These findings demonstrate a role for a mitochondrial protein deacetylase in hippocampal neurons in behavioral and GABAergic synaptic adaptations to IF.
Characterizing pre-transplant and post-transplant kidney rejection risk by B cell immune repertoire sequencing
Authors: Pineda Silvia , Sigdel Tara K. , Liberto Juliane M. , Vincenti Flavio , Sirota Marina , Sarwal Minnie M. 
Published: April 2019

AbstractStudying immune repertoire in the context of organ transplant provides important information on how adaptive immunity may contribute and modulate graft rejection. Here we characterize the peripheral blood immune repertoire of individuals before and after kidney transplant using B cell receptor sequencing in a longitudinal clinical study. Individuals who develop rejection after transplantation have a more diverse immune repertoire before transplant, suggesting a predisposition for post-transplant rejection risk. Additionally, over 2 years of follow-up, patients who develop rejection demonstrate a specific set of expanded clones that persist after the rejection. While there is an overall reduction of peripheral B cell diversity, likely due to increased general immunosuppression exposure in this cohort, the detection of specific IGHV gene usage across all rejecting patients supports that a common pool of immunogenic antigens may drive post-transplant rejection. Our findings may have clinical implications for the prediction and clinical management of kidney transplant rejection.
Binary tree-inspired digital dendrimer
Authors: Huang Zhihao , Shi Qiunan , Guo Jiang , Meng Fanying , Zhang Yajie , Lu Yutong , Qian Zhuangfei , Li Xiaopeng , Zhou Nianchen , Zhang Zhengbiao , Zhu Xiulin 
Published: April 2019

AbstractDigital polymers with precisely ordered units acting as the coded 0- or 1-bit, are introduced as a promising option for molecular data storage. However, the pursuit of better performance in terms of high storage capacity and useful functions never stops. Herein, we propose a concept of an information-coded 2D digital dendrimer. The divergent growth via thiol-maleimide Michael coupling allows precise arrangements of the 0- and 1-bits in the uniform dendrimers. A protocol for calculating the storage capacity of non-linear binary digital dendrimer is established based on data matrix barcode, generated by the tandem mass spectrometry decoding and encryption. Furthermore, the generated data matrix barcode can be read by a common hand-held device to cater the applications such as item identification, traceability and anticouterfeiting purpose. This work demonstrates the high data storage capacity of a uniform dendrimer and uncovers good opportunities for the digital polymers.
Loading a quantum-dot based “Qubyte” register
Authors: Volk C. , Zwerver A. M. J. , Mukhopadhyay U. , Eendebak P. T. , van Diepen C. J. , Dehollain J. P. , Hensgens T. , Fujita T. , Reichl C. , Wegscheider W. , Vandersypen L. M. K. 
Published: April 2019

AbstractElectrostatically defined quantum dot arrays offer a compelling platform for quantum computation and simulation. However, tuning up such arrays with existing techniques becomes impractical when going beyond a handful of quantum dots. Here, we present a method for systematically adding quantum dots to an array one dot at a time, in such a way that the number of electrons on previously formed dots is unaffected. The method allows individual control of the number of electrons on each of the dots, as well as of the interdot tunnel rates. We use this technique to tune up a linear array of eight GaAs quantum dots such that they are occupied by one electron each. This new method overcomes a critical bottleneck in scaling up quantum-dot based qubit registers.
Long-Term Efficacy and Safety of Evocalcet in Japanese Patients with Secondary Hyperparathyroidism Receiving Hemodialysis
Authors: Yokoyama Keitaro , Shimazaki Ryutaro , Fukagawa Masafumi , Akizawa Tadao ,   On behalf of Evocalcet Study Group, Maeda Yoshitaka , Ueki Kazue , Fujii Takayuki , Miyazaki Ryoichi , Azekura Hisanori , Kasuga Hirotake , Tomiyoshi Yoshiyuki , Shinzato Takeaki , Iwashita Ryuji , Takada Kenji , Suda Akio , Nagaoka Takashi , Yoshimoto Mitsuru , Taniguchi Masatomo , Ogawa Hiroshi 
Published: April 2019

AbstractSecondary hyperparathyroidism (SHPT) is a common complication of chronic kidney disease (CKD), and as the disease progresses SHPT is associated with systemic consequences, termed CKD-mineral and bone disorder. Currently, cinacalcet is indicated for the treatment of SHPT; however, cinacalcet is associated with upper gastrointestinal adverse events. Evocalcet has been developed to address these issues, but the long-term safety and efficacy of evocalcet need to be evaluated. To more accurately reflect clinical practice, this phase 3, multicenter, open-label study was specifically designed without a cinacalcet washout period, and focused on those patients who switched from cinacalcet to evocalcet. A total of 137 SHPT patients undergoing hemodialysis were enrolled, of whom 113 switched from cinacalcet to evocalcet. The most frequent type of adverse drug reaction was decreased adjusted calcium. The incidence of gastrointestinal-related adverse events did not increase in a dose-dependent manner as the dose of evocalcet was increased. The percentage of patients achieving the target intact parathyroid hormone concentration increased from 40.9% to 72.3% with 52-week treatment. The corrected serum calcium and phosphorus levels remained largely unchanged throughout the study. The long-term safety and efficacy of evocalcet was confirmed using a clinically relevant intra-subject dose-adjustment strategy in SHPT patients undergoing hemodialysis.
A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy
Authors: Huerta-Nuñez L. F. E. , Gutierrez-Iglesias G. , Martinez-Cuazitl A. , Mata-Miranda M. M. , Alvarez-Jiménez V. D. , Sánchez-Monroy V. , Golberg Alexander , González-Díaz C. A. 
Published: April 2019

AbstractBreast cancer (BC) is a malignant disease with a high prevalence worldwide. The main cause of death is not the primary tumor, but instead the spread of tumor cells to distant sites. The aim of the present study was to examine a new method for the detection of cancer cells in aqueous medium using bioimpedance spectroscopy assisted with magnetic nanoparticles (MNP’s) exposure to a constant magnetic field. The spectroscopic patterns were identified for three breast cancer cell lines. Each BC cell line represents a different pathologic stage: the early stage (MCF-7), invasive phase (MDA-MB-231) and metastasis (SK-BR-3). For this purpose, bioimpedance measurements were carried out at a certain frequency range with the aid of nanoprobes, consisting of magnetic nanoparticles (MNPs) coupled to a monoclonal antibody. The antibody was specific for the predominant cell surface protein for each cell line, which was identified by using RT-qPCR and flow cytometry. Accordingly, EpCAM corresponds to MCF-7, MUC-1 to MDA-MB-231, and HER-2 to SK-BR-3. Despite their low concentrations, BC cells could be detected by impedance spectroscopy. Hence, this methodology should permit the monitoring of circulating tumor cells (CTC) and therefore help to prevent recurrences and metastatic processes during BC treatment.
Retention of beneficial molecules and coagulation factors during haemodialysis and haemodiafiltration
Authors: Voigt Manuel , Gebert Michael , Haug Ulrike , Hulko Michael , Storr Markus , Boschetti-de-Fierro Adriana , Beck Werner , Krause Bernd 
Published: April 2019

AbstractMiddle molecules (MMs) are associated with the pathology of uraemia, and are not effectively removed by standard extracorporeal treatments. Increased convection used in haemodiafiltration (HDF) can enhance the removal of MMs; however, high-volume HDF is not available to all patients. The new medium cut-off (MCO) membrane has been developed to allow increased removal of MMs using standard haemodialysis (HD). Improved removal of MMs has been shown with the MCO membrane compared with standard high-flux dialysers, but it is not known whether the increased pore size affects the retention of commonly used medications or that of coagulation factors in dialysis patients. Using an in vitro model, the retention of erythropoietin, heparin, insulin, vancomycin and several coagulation factors (Factors II, VII and X, protein C and antithrombin III) was investigated with the MCO membrane dialyser, compared with high-flux dialysers with polysulfone (in HDF) or polyethersulfone membranes (in HD and HDF). The retention of all molecules investigated was comparable between the MCO membrane and the high-flux dialysers. Results from the in vitro studies suggest that switching from a high-flux dialyser to the MCO membrane should not require changes to the medication dosing or anti-coagulation protocols of dialysis patients.
A novel wireless brain stimulation device for long-term use in freely moving mice
Authors: Alpaugh Melanie , Saint-Pierre Martine , Dubois Marilyn , Aubé Benoit , Arsenault Dany , Kriz Jasna , Cicchetti Antonio , Cicchetti Francesca 
Published: April 2019

AbstractDeep brain stimulation (DBS) has been used in clinical settings for many years despite a paucity of knowledge related to the anatomical and functional substrates that lead to benefits and/or side-effects in various disease contexts. In order to maximize the potential of this approach in humans, a better understanding of its mechanisms of action is absolutely necessary. However, the existing micro-stimulators available for pre-clinical models, are limited by the lack of relevant small size devices. This absence prevents sustained chronic stimulation and real time monitoring of animals during stimulation, parameters that are critical for comparison to clinical findings. We therefore sought to develop and refine a novel small wireless micro-stimulator as a means by which to study consequent behavioural to molecular changes in experimental animals. Building on previous work from our group, we refined our implantable micro-stimulator prototype, to be easily combined with intravital 2-photon imaging. Using our prototype we were able to replicate the well described clinical benefits on motor impairment in a mouse model of Parkinson’s disease in addition to capturing microglia dynamics live during stimulation. We believe this new device represents a useful tool for performing pre-clinical studies as well as dissecting brain circuitry and function.
In vitro differentiation of single donor derived human dental mesenchymal stem cells into pancreatic β cell-like cells
Authors: Shivakumar Sharath B. , Lee Hyeon-Jeong , Son Young-Bum , Bharti Dinesh , Ock Sun A. , Lee Sung-Lim , Kang Young-Hoon , Park Bong-Wook , Rho Gyu-Jin 
Published: April 2019

Abstract
The present study was carried out to investigate and compare the in vitro differentiation potential of mesenchymal stem cells (MSCs) isolated from human dental tissues (pulp, papilla, and follicle) of the same donor. MSCs were isolated from dental tissues (pulp, papilla, and follicle) following digestion method and were analyzed for the expression of pluripotent markers and cell surface markers. All three types of MSCs were evaluated for their potential to differentiate into mesenchymal lineages. Further, the MSCs were differentiated into pancreatic β cell-like cells using multistep protocol and characterized for the expression of pancreatic lineage specific markers. Functional properties of differentiated pancreatic β cell-like cells were assessed by dithizone staining and glucose challenge test. All three types of MSCs showed fibroblast-like morphology upon culture and expressed pluripotent, and mesenchymal cell surface markers. These MSCs were successfully differentiated into mesenchymal lineages and transdifferentiated into pancreatic β cell-like cells. Among them, dental follicle derived MSCs exhibits higher transdifferentiation potency toward pancreatic lineage as evaluated by the expression of pancreatic lineage specific markers both at mRNA and protein level, and secreted higher insulin upon glucose challenge. Additionally, follicle-derived MSCs showed higher dithizone staining upon differentiation. All three types of MSCs from a single donor possess similar cellular properties and can differentiate into pancreatic lineage. However, dental follicle derived MSCs showed higher potency toward pancreatic lineage than pulp and papilla derived MSCs, suggesting their potential application in future stem cell based therapy for the treatment of diabetes.
MiR-19a as a prognostic indicator for cancer patients: a meta-analysis
Authors: Peng Yizhong , Huang Donghua , Ma Kaige , Deng Xiangyu , Shao Zengwu 
Published: April 2019

Abstract
MiR-19a was aberrantly expressed in various types of cancers and was observed to be potentially associated with the prognosis of cancer patients. The present analysis aims to elucidate its precise predictive value in various human malignancies. Online electronic searches of PubMed, Web of Science (WOS), Embase in English and VIP, Wanfang, SinoMed, and the China National Knowledge Infrastructure (CNKI) in Chinese up to September 8, 2018 were conducted. As a result, in overall analysis, a significant association was identified between miR-19a levels and OS (HRs = 2.31, CI: 1.11–4.83). The relation of miR-19a expression to OS was further recognized by fixed model within the studies of sample size less than 150 (HRs = 1.68, CI: 1.35–2.08), NOS scores greater than or equal to 8 (HRs = 1.53, CI: 1.13–2.06) or less than 8 (HRs = 1.89, CI: 1.58–2.27), specimen derived from tumor (HRs = 1.73, CI: 1.42–2.12) or blood (HRs = 1.87, CI: 1.46–2.40) and the patients of osteosarcoma (HRs = 7.17, CI: 5.04–10.21). Sensitivity analyses revealed no significant results. The association between miR-19a expression level and DFS was also found to be significant (HRs = 2.03, CI: 1.13–3.66). Correlations between miR-19a levels and clinicopathological features were examined and revealed that lymph node metastasis was significantly associated with miR-19a expression levels (OR = 0.565, CI: 0.346–0.921). Summarily, the over expression of miR-19a was an underlying risk of poor prognosis in many human malignancies, especially in osteosarcoma. Moreover, elevated miR-19a expression was linked to the potential of lymph node metastasis.
Mitochondrial protein 18 is a positive apoptotic regulator in cardiomyocytes under oxidative stress
Authors: Aung Lynn H.H. , Li Yu-Zhen , Yu Hua , Chen Xiatian , Yu Zhongjie , Gao Jinning , Li Peifeng 
Published: April 2019

Abstract
Accumulation of reactive oxygen species is a common phenomenon in cardiac stress conditions, for instance, coronary artery disease, aging-related cardiovascular abnormalities, and exposure to cardiac stressors such as hydrogen peroxide (H2O2). Mitochondrial protein 18 (Mtp18) is a novel mitochondrial inner membrane protein, shown to involve in the regulation of mitochondrial dynamics. Although Mtp18 is abundant in cardiac muscles, its role in cardiac apoptosis remains elusive. The present study aimed to detect the role of Mtp18 in H2O2-induced mitochondrial fission and apoptosis in cardiomyocytes. We studied the effect of Mtp18 in cardiomyocytes by modulating its expression with lentiviral construct of Mtp18-shRNA and Mtp18 c-DNA, respectively. We then analyzed mitochondrial morphological dynamics with MitoTracker Red staining; apoptosis with terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling (TUNEL) and cell death detection assays; and protein expression with immunoblotting. Here, we observed that Mtp18 could regulate oxidative stress- mediated mitochondrial fission and apoptosis in cardiac myocytes. Mechanistically, we found that Mtp8 induced mitochondrial fission and apoptosis by enhancing dynamin-related protein 1 (Drp1) accumulation. Conversely, knockdown of Mtp18 interfered with Drp1-associated mitochondrial fission and subsequent activation of apoptosis in both HL-1 cells and primary cardiomyocytes. However, overexpression of Mtp18 alone was not sufficient to execute apoptosis when Drp1 was minimally expressed, suggesting that Mtp18 and Drp1 are interdependent in apoptotic cascade. Together, these data highlight the role of Mtp18 in cardiac apoptosis and provide a novel therapeutic insight to minimize cardiomyocyte loss via targetting mitochondrial dynamics.
Teamspirit stärken
Authors: Welk Ina 
Published: April 2019

ZusammenfassungEin echtes „Wir“-Gefühl im OP zu entwickeln ist aufgrund der häufig wechselnden
Zusammensetzung des Teams herausfordernd. Wie es dennoch gelingt,
unterschiedliche Erfahrungen, Qualifikationen und Fertigkeiten unter einen Hut
zu bringen und als Team zusammenzuwachsen, erläutern wir im Schwerpunkt. Denn
effektives Teamwork ist kein Zufall, sondern bedarf einer strukturierten
Teamentwicklung.
Schlittenprothese
Authors: Aue Michelle , Hübner Jochen 
Published: April 2019

ZusammenfassungSchlittenprothesen werden bereits seit 1970 an der Knieinnenseite (medial) oder
an der Knieaußenseite (lateral) implantiert. Der teilweise Gelenkersatz ist ein
erhaltender, kein ersetzender Eingriff, da das Kniegelenk nicht voll erneuert,
sondern nur wenige Teile ersetzt werden. Es handelt sich hierbei um die kleinste
Variante einer Endoprothese am Kniegelenk.
Prophylaxe und Therapie des Arm-Lymphödems
Authors: Brenke Rainer 
Published: April 2019

SummaryDas Arm-Lymphödem ist trotz schonenderer Operationsmethoden auch heute noch eine schwerwiegende Komplikation der Therapie des Mammakarzinoms. Behandlung erster Wahl ist die Komplexe Physikalische Entstauungstherapie mit Lymphdrainage, Kompression, Bewegung und Hautpflege.
Neue Arzneimittel für Kleintiere 2018
Authors: Emmerich Ilka Ute 
Published: April 2019

ZusammenfassungIm Jahr 2018 kamen für Kleintiere 2 neue Wirkstoffe auf den deutschen
Tierarzneimittelmarkt: das Ektoparasitikum der Isoxazolingruppe Lotilaner
(Credelio®) und das Opioidanalgetikum Tramadol
(Tralieve®). Zwei Wirkstoffe erhielten eine Tierartenerweiterung.
So wurde das nicht steroidale Antiphlogistikum Meloxicam (Metacam®)
aus der Gruppe der Oxicame auch für Meerschweinchen und das
Inhalationsnarkotikum Sevofluran (Sevoflo®) aus der Gruppe der
halogenierten Kohlenwasserstoffverbindungen auch für Katzen zugelassen. Mit
Benzylpenicillin-Benzathin in Kombination mit Benzylpenicillin-Procain wurde
eine zwischenzeitlich nicht mehr erhältliche Wirkstoffkombination wieder
zugelassen. Außerdem kamen ein Wirkstoff in einer neuen Darreichungsform und ein
Präparat mit einem neuen Wirkstoffgehalt für Kleintiere auf den Markt.
Psychometrische Überprüfung der Kurzversion des „Systems of Belief Inventory“ in einer deutschlandweiten repräsentativen Bevölkerungsstichprobe
Authors: Hampel Nicola Daniela , Brähler Elmar , Zenger Markus , Albani Cornelia , Heuft Gereon 
Published: April 2019

Zusammenfassung
Einleitung In dieser Arbeit wird die Entwicklung der deutschen Version des „Systems of Belief Inventory“ in der revidierten und gekürzten 6 Item Version (SBI-6R-D; Heuft und Hampel 2017) vorgestellt und psychometrisch überprüft.
Material und Methoden Im Rahmen einer deutschlandweiten Repräsentativbefragung wurde der Fragebogen 2513 Probanden vorgelegt. Eine konfirmatorische Faktorenanalyse wurde durchgeführt, um die Faktorenstruktur im Vergleich zur ursprünglichen amerikanischen Version zu überprüfen. Messinvarianz wurde über Multigruppenvergleiche, die konvergente und diskriminante Validität wurden über Korrelationen mit anderen Items getestet.
Ergebnisse Es ergaben sich gute Item- und Skalenkennwerte sowie eine gute Modellgüte. Im Unterschied zur Arbeit von Albani et al. (SBI-15R-D) konnte jetzt die 2- faktorielle Struktur des US-amerikanischen Fragebogens repliziert werden. Für Geschlecht und Alter wird skalare Invarianz, für Konfession und Bundeslandzugehörigkeit nur metrische Invarianz gezeigt.
Diskussion Aufgrund der Güte der bisherigen Kennwerte des SBI-6R-D erscheint es lohnend, den SBI-6R-D in zukünftigen Untersuchungen mit anderen validierten religiösen Vergleichsinstrumenten weitergehend zu untersuchen.
Schlussfolgerung Mit dieser neuen deutschen Kurzversion SBI-6R-D liegt ein ökonomisches, reliables und valides Instrument zur Erfassung spiritueller und religiöser Einstellungen vor.
Die Integrative Behandlung der Frozen Shoulder
Authors: Koll Steffen , Gnauert Andreas , Seidel Wolfram 
Published: April 2019

SummaryDie „Frozen Shoulder“ ist eine Form der Schultersteife, die phasenhaft verläuft und bei den Betroffenen zu Schmerzen und Bewegungseinschränkungen führt. Eine genaue Struktur- und Funktionsdiagnostik und die Berücksichtigung möglicher psychosozialer Einflussfaktoren sind für die Therapieplanung erforderlich. Bei der Behandlung der Frozen Shoulder kommen neben Schmerztherapie, Physiotherapie inklusive Krankengymnastik, Manueller Therapie und Eigenübungen auch komplementäre Verfahren wie Akupunktur oder Blutegel-Behandlungen zum Einsatz. Multimodale, komplexe Behandlungskonzepte können notwendig sein.