bluuebunny/crossref_metadata_2025_split huggingface dataset:
A cancer-associated polymorphism in ESCRT-III disrupts the abscission checkpoint and promotes genome instability
Authors: Sadler Jessica B.A. , Wenzel Dawn M. , Williams Lauren K. , Guindo-Martínez Marta , Alam Steven L. , Mercader Josep Maria , Torrents David , Ullman Katharine S. , Sundquist Wesley I. , Martin-Serrano Juan 
Published: July 2018

AbstractCytokinetic abscission facilitates the irreversible separation of daughter cells. This process requires the Endosomal Sorting Complexes Required for Transport (ESCRT) machinery and is tightly regulated by Charged Multivesicular body Protein 4C (CHMP4C), an ESCRT-III subunit that engages the abscission checkpoint (NoCut) in response to mitotic problems such as persisting chromatin bridges within the midbody. Importantly, a human polymorphism in CHMP4CT232 (rs35094336), increases cancer susceptibility. Here, we explain the structural and functional basis for this cancer association: the CHMP4CT232 allele unwinds the C-terminal helix of CHMP4C, impairs binding to the early-acting ESCRT factor ALIX, and disrupts the abscission checkpoint. Cells expressing CHMP4CT232 exhibit increased levels of DNA damage and are sensitized to several conditions that increase chromosome mis-segregation, including DNA replication stress, inhibition of the mitotic checkpoint, and loss of p53. Our data demonstrate the biological importance of the abscission checkpoint, and suggest that dysregulation of abscission by CHMP4CT232 may synergize with oncogene-induced mitotic stress to promote genomic instability and tumorigenesis.Significance StatementThe final step of cell division, abscission, is temporally regulated by the Aurora B kinase and CHMP4C in a conserved pathway called the abscission checkpoint which arrests abscission in the presence of lingering mitotic problems. Despite extensive study, the physiological importance of this pathway to human health has remained elusive. We now demonstrate that a cancer predisposing polymorphism in CHMP4C disrupts the abscission checkpoint and results in DNA damage accumulation. Moreover, deficits in this checkpoint synergize with p53 loss and generate aneuploidy under stress conditions that increase the frequency of chromosome missegregation. Therefore, cells expressing the cancer-associated polymorphism in CHMP4C are genetically unstable, thus suggesting a novel oncogenic mechanism that may involve the dysregulation of abscission.
Microtubules provide a viscoelastic resistance to myocyte motion
Authors: Caporizzo M. A. , Chen C. Y. , Salomon A. K. , Bedi K. , Margulies K. B. , Prosser B. L. 
Published: July 2018

ABSTRACTBackgroundMicrotubules (MT) buckle and bear load during myocyte contraction, a behavior enhanced by post-translational detyrosination. This buckling suggests a spring-like resistance against myocyte shortening, which could store energy and aid myocyte relaxation. Despite this visual suggesting of elastic behavior, the precise mechanical contribution of the cardiac MT network remains to be defined.MethodsHere we experimentally and computationally probe the mechanical contribution of stable microtubules and their influence on myocyte function. We use multiple approaches to interrogate viscoelasticity and cell shortening in primary murine myocytes where either MTs are depolymerized or detyrosination is suppressed, and use the results to inform a mathematical model of myocyte viscoelasticity.ResultsMT ablation by colchicine concurrently enhances both the degree of shortening and speed of relaxation, a finding inconsistent with simple spring-like microtubule behavior, and suggestive of a viscoelastic mechanism. Axial stretch and transverse indentation confirm that microtubules increase myocyte viscoelasticity. Specifically, increasing the rate of strain amplifies the MT contribution to myocyte stiffness. Suppressing MT detyrosination with parthenolide or via overexpression of tubulin tyrosine ligase (TTL) has mechanical consequences that closely resemble colchicine, suggesting that the mechanical impact of MTs relies on a detyrosination-dependent linkage with the myocyte cytoskeleton. Mathematical modeling affirms that alterations in cell shortening conferred by either MT destabilization or tyrosination can be attributed to internal changes in myocyte viscoelasticity.ConclusionsThe results suggest that the cardiac MT network regulates contractile amplitudes and kinetics by acting as a cytoskeletal shock-absorber, whereby MTs provide breakable cross-links between the sarcomeric and non-sarcomeric cytoskeleton that resist rapid length changes during both shortening and stretch.
LiF-MS: Mapping unstructured peptide-protein interactions using Ligand-Footprinting Mass Spectrometry
Authors: Parker Benjamin , Goncz Edward , Krist David T. , Statsyuk Alexander , Nesvizhskii Alexey I. , Weiss Eric 
Published: July 2018

AbstractUnstructured peptides, or linear motifs, present a poorly understood molecular language within the context of cellular signaling. These modular regions are often short, unstructured and interact weakly and transiently with folded target proteins. Thus, they are difficult to study with conventional structural biology methods. We present Ligand-Footprinting Mass Spectrometry, or LiF-MS, as a method of mapping the binding sites and dynamic disorder of these peptides on folded protein domains. LiF-MS uses a cleavable crosslinker to mark regions of a protein contacted by a bound linear motif. We demonstrate this method can detect both conformation ensembles and binding orientations of a linear motif in its binding pocket to amino-acid-level detail. Furthermore, marked amino acids can be used as constraints in peptide-protein docking simulations to improve model quality. In conclusion, LiF-MS proves a simple and novel method of elucidating peptide docking structural data not accessible by other methods in the context of a purified system.
Understanding Finnish student craft teachers’ conceptions of sustainability
Authors: Väänänen Niina , Vartiainen Leena , Kaipainen Minna , Pitkäniemi Harri , Pöllänen Sinikka 
Published: July 2018

Purpose
This study aims to explore student craft teachers’ conceptions of sustainable craft. This is an important issue because the Finnish National Curriculum of Basic Education emphasises sustainability, especially in craft education, and teachers play a vital role in preparing pupils to meet the future challenges. Because the concept of sustainable craft is open-ended, there is a need to rethink pedagogy in craft education and higher education.

Design/methodology/approach
The data were collected in the form of essays from future craft teachers (N41) studying craft science in the Finnish higher education system. The essays concerned both general conceptions of sustainable craft and reflections on the student teachers’ craft practices. The data were analysed using grounded theory to gain a deep understanding of how student craft teachers conceptualise sustainable craft. The data were quantified and statistically assessed for dependencies between variables and transferability of results.

Findings
The study revealed that sustainable craft is conceptualised as a system and that student teachers approach sustainability from different orientations: practice, product, immaterial and holistic.

Originality/value
The emerging theory offers a new concrete tool for understanding the abstract concept of sustainability in higher education and suggests that sustainability can be addressed through tangible methods of craft. This theory proposes craft as a tool to conceptualise of sustainability for broader use in education for sustainability (ESD) and as a concrete tool for developing pedagogy for ESD.
Tourist cities as embodied places of learning: walking in the “feelds” of Shanghai and Lisbon
Authors: Küpers Wendelin , Wee Desmond 
Published: July 2018

Purpose
Walking is considered as a particular relationship for rhythmic moving in cityscapes and as method for understanding. The purpose of this paper is to explore the significance of an embodied way of sensing and making sense, of knowing and learning that is relevant for tourism education and studies and other forms of experiential learning.

Design/methodology/approach
The paper uses a conceptual, discursive and research-based approach based on a phenomenological understanding of embodied learning. Empirical “data” from two educational tours (Edutour) in Lisbon and Shanghai are presented, contextualized and interpreted.

Findings
Walking through cityscape related to projects is an “effective” embodied practice of learning in which senses “make sense.” The empirical material and experiences that emerged during Edutour demonstrated the significance of walking as form of embodied knowing, learning and interrelating to place and paces of a tourist city.

Originality/value
The idea of walking as method within tourist cities is hardly explored. Hence, this constitutes a unique, innovative and interpretative event in which new approaches, such as “fielding” and “reflactions” “in the feeld”, defy more traditional concepts. It emphasizes the role of the city as a medium and remains a valuable contribution for tourism and education research.
Identification and evaluation for the dynamic signals caused by pressure fluctuation of aerostatic slider
Authors: Chen Dongju , Han Jihong , Cui Xianxian , Fan Jinwei 
Published: July 2018

Purpose
To identify the dynamic feature of the aerostatic slider caused by gas film, an evaluation system by a piezoelectric acceleration sensor is presented in time and frequency domain.

Design/methodology/approach
The dynamic pressure fluctuation is evaluated by the wavelet transform, cross correlation analysis and power spectral density (PSD). Wavelet transform is used to process the measured result of the aerostatic slider and the signal is decomposed into high-frequency and low-frequency signal. Correlation analysis method is used to evaluate the impact of the initial gas gap on the fluctuation in time domain.

Findings
According to the PSD analysis of the processed signal in the frequency domain, the natural frequency of the aerostatic slider is identified from the measured signal in frequency domain; this method provides a basis for the identification of guideway errors.

Research limitations/implications
The method can also be applied to the error identification of other components of the machine tool.

Originality/value
Wavelet transform is used to process the measured result of the aerostatic slider by acceleration sensor, and the signal is decomposed into high-frequency and low-frequency signal. Correlation analysis method is used to evaluate the impact of the initial gas gap on the fluctuation in time domain. According to the PSD analysis of the processed signal in the frequency domain, the natural frequency of the aerostatic slider is identified from the measured signal in frequency domain; this method provides a basis for the identification of slider errors.
A cross-platform parallel programming model for fluid-film lubrication optimization
Authors: Wang Nen-Zi , Chen Hsin-Yi 
Published: July 2018

Purpose
A cross-platform paradigm (computing model), which combines the graphical user interface of MATLAB and parallel Fortran programming, for fluid-film lubrication analysis is proposed. The purpose of this paper is to take the advantages of effective multithreaded computing of OpenMP and MATLAB’s user-friendly interface and real-time display capability.

Design/methodology/approach
A validation of computing performance of MATLAB and Fortran coding for solving two simple sliders by iterative solution methods is conducted. The online display of the particles’ search process is incorporated in the MATLAB coding, and the execution of the air foil bearing optimum design is conducted by using OpenMP multithreaded computing in the background. The optimization analysis is conducted by particle swarm optimization method for an air foil bearing design.

Findings
It is found that the MATLAB programs require prolonged execution times than those by using Fortran computing in iterative methods. The execution time of the air foil bearing optimum design is significantly minimized by using the OpenMP computing. As a result, the cross-platform paradigm can provide a useful graphical user interface. And very little code rewritting of the original numerical models is required, which is usually optimized for either serial or parallel computing.

Research limitations/implications
Iterative methods are commonly applied in fluid-film lubrication analyses. In this study, iterative methods are used as the solution methods, which may not be an effective way to compute in the MATLAB’s setting.

Originality/value
In this study, a cross-platform paradigm consisting of a standalone MATLAB and Fortran codes is proposed. The approach combines the best of the two paradigms and each coding can be modified or maintained independently for different applications.
Contact is a stronger predictor of attitudes toward police than race: a state-of-the-art review
Authors: Alberton Amy M. , Gorey Kevin M. 
Published: July 2018

Purpose
This scoping review thoroughly scanned research on race, contacts with police and attitudes toward police. An exploratory meta-analysis then assessed the strength of their associations and interaction in Canada and the USA. Key knowledge gaps and specific future research needs, synthetic and primary, were identified. The paper aims to discuss these issues.

Design/methodology/approach
A germinal methodological framework for conducting scoping reviews was used (Arksey and O’Malley, 2005). The authors searched for published or unpublished research over the past 15 years and retrieved 33 eligible surveys, 19 of which were included in a sample-weighted meta-analysis.

Findings
The independent association of contact with attitudes toward police was estimated to be three times larger than the independent race association. Three large knowledge gaps were identified. Almost nothing is known about these associations among specific racial groups as they were typically aggregated into visible minority groupings. The authors have essentially no knowledge yet about specific racial group by a specific type of contact interactions. There is also a lack of generalizable knowledge as research has been largely restricted to locales.

Originality/value
This is the first research synthesis of race and attitudes toward the police that incorporated contacts with the police. Its observation of the relative importance of contacts suggested a great preventive potential. This scoping review identified needs for a full systematic research review and a formal meta-analysis to plan future primary research including large national studies that are truly representative of Canada and America’s diversity. Such will be needed to advance more confident knowledge about the factors that would support more trusted relationships between police and people in the communities they aim to serve.
A Weighted Displacement Quotient model for understanding the impact of Crime Prevention through Environmental Design
Authors: Cho Il-Hyoung , Jung Kyujin 
Published: July 2018

Purpose
The purpose of this paper is to investigate the impact of Crime Prevention Through Environmental Design (CPTED), a crime prevention tool, on reducing rates of sexual assault. In addition, the study attempts to understand if CPTED results in crime displacement in non-target areas.

Design/methodology/approach
This research utilizes a Weighted Displacement Quotient (WDQ) model to analyze the effects of CPTED, which is an appropriate tool in fields of regional-scale crime prevention and on sexual assault prevention. WDQ is capable of analyzing policy effectiveness while controlling for geographical crime displacement, a known side effect of CPTED in the literature.

Findings
The analysis results show that CPTED is an effective tool to prevent sexual assaults in South Korea. The sexual assault occurrence rate decreased in the CPTED implementation zone of Yeomri-dong Mapo-gu. WDQ showed that crime displacement occurred in adjacent areas in Daeheung-dong and Ahyun-dong. But, crime displacement was lower than the policy effectiveness in the target zone.

Originality/value
The policy implications of this research are immense. First, CPTED for the prevention of sexual assaults should be considered as a pre-control tool. Second, a strategic method for more effectively implementing CPTED is required. Third, because CPTED is a policy done on a regional scale, provisions need to be in place to manage crime displacement.
Individual and situational influences on injurious assaults against the police
Authors: Barrick Kelle , Strom Kevin J. , Richardson Nicholas 
Published: July 2018

Purpose
Violence against the police represents an ongoing and serious problem in the USA. In 2014, over 48,000 law enforcement officers assaulted while on duty. Although over one in four of these resulted in injury, little is known about the conditions under which injury is likely to occur. The purpose of this paper is to provide an assessment of the individual and situational factors that predict injurious assaults against law enforcement.

Design/methodology/approach
Using logistic regression, the current study analyzes data from the 2012 National Incident-Based Reporting System (NIBRS) on all assaults against the police (n=8,987) in order to understand, within a routine activities theoretical framework, how individual-level characteristics (i.e. officer and offender characteristics) and situational influences (i.e. assignment type, activity type, and location) predict the likelihood that an assault will result in injury.

Findings
Overall, findings suggest support for a routine activities theory of violence against the police. Initiating an arrest, one-officer vehicle type, and incidents occurring on highways/roads were all more likely to result in injurious assaults against the police. Other predictors of injury include officer and offender demographics as well as the time the incident took place.

Research limitations/implications
This research was unable to control for some factors that may influence the likelihood of injury such as wearing body armor. Additionally, NIBRS data are not nationally representative, which limits the generalizability of the findings.

Originality/value
This is one of the first papers to use national data to examine the individual and situational factors that predict injurious assaults against law enforcement.
Data and evidence challenges facing place-based policing
Authors: Hutt Oliver , Bowers Kate , Johnson Shane , Davies Toby 
Published: July 2018

Purpose
The purpose of this paper is to use an evaluation of a micro-place-based hot-spot policing implementation to highlight the potential issues raised by data quality standards in the recording and measurement of crime data and police officer movements.

Design/methodology/approach
The study focusses on an area of London (UK) which used a predictive algorithm to designate micro-place patrol zones for each police shift over a two-month period. Police officer movements are measured using GPS data from officer-worn radios. Descriptive statistics regarding the crime data commonly used to evaluate this type of implementation are presented, and simple analyses are presented to examine the effects of officer patrol duration (dosage) on crime in micro-place hot-spots.

Findings
The results suggest that patrols of 10-20 minutes in a given police shift have a significant impact on reducing crime; however, patrols of less than about 10 minutes and more than about 20 minutes are ineffective at deterring crime.

Research limitations/implications
Due to the sparseness of officer GPS data, their paths have to be interpolated which could introduce error to the estimated patrol dosages. Similarly, errors and uncertainty in recorded crime data could have substantial impact on the designation of micro-place interventions and evaluations of their effectiveness.

Originality/value
This study is one of the first to use officer GPS data to estimate patrol dosage and places particular emphasis on the issue of data quality when evaluating micro-place interventions.
Institutionalizing place-based policing: the adoption of a Case of Place approach
Authors: Bond Brenda J. , Nader Elias 
Published: July 2018

PurposeResearch shows that crime and disorder tend to concentrate in small, geographic locations and that place-based and problem-solving policing strategies can impact crime and disorder without displacing it to neighboring areas. However, implementation of problem-solving is a challenge. Loosely defined locations, shallow problem analysis, and distractions to problem-solving are cited implementation shortcomings. These shortcomings may be overcome by using the Case of Place approach, a case management strategy focused on documenting and analyzing place-based dynamics and characteristics to inform and direct policing strategies. The paper aims to discuss these issues.Design/methodology/approachThe current study describes the adoption of the Case of Place approach in an urban police agency’s operations and performance management system. The authors utilize implementation theory to explore and explain the adoption of this new place-based strategy.FindingsKey findings reveal important structural and cultural challenges to implementation. Structural challenges included modifying supervision structures, creating new positions, decentralizing analytical functions, and redirecting resources to problem-solving. Cultural challenges observed included emphasizing problem-solving as an organizational priority, integrating crime analysts into neighborhood precincts, and centering performance management processes around problem-solving.Originality/valueThe authors explore how implementation dynamics impact the adoption of new policies and practices, and offer a number of propositions for the use of the Case of Place approach within a place-based strategy portfolio.
Porosity measurement of heavy oil sands
Authors: Yuan Hemin , Han De‐Hua , Huang Qi 
Published: July 2018

ABSTRACTPorosity measurement is an important step for petrophysical characterization of reservoir rocks, as porosity is essential for analysing elastic properties and estimating the capacity of rocks to hold hydrocarbon resources. Commonly used porosity measurement methods fail to work on heavy oil sands, because of the loose fragile sand frame and solid‐like irreducible heavy oil. We develop three methods to measure the porosity of heavy oil sands, which are feasible and affordable in most laboratories. Method 1 involves calculating the bulk volume of the sample by measuring its physical dimensions directly. Method 2 uses calibrated sample weight and volume (after removing the protecting foil cover and metal clips). Method 3 first applies pressurizing on samples and then measures the weight and volume of the bare samples. The measurement results of the three methods are then compared and method 3 is determined as the most reliable, which is also verified by the porosity log. Finally, an analysis of the potential sources of errors associated with method 3 is performed.