bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extended indirect calorimetry with isotopic CO2 sensors for prolonged and continuous quantification of exogenous vs. total substrate oxidation in mice
Authors: Fernández-Calleja José M. S. , Bouwman Lianne M. S. , Swarts Hans J. M. , Oosting Annemarie , Keijer Jaap , van Schothorst Evert M. 
Published: August 2019

AbstractIndirect calorimetry (InCa) estimates whole-body energy expenditure and total substrate oxidation based on O2 consumption and CO2 production, but does not allow for the quantification of oxidation of exogenous substrates with time. To achieve this, we incorporated 13CO2 and 12CO2 gas sensors into a commercial InCa system and aimed to demonstrate their performance and added value. As a performance indicator, we showed the discriminative oscillations in 13CO2 enrichment associated with food intake in mice fed diets containing naturally low (wheat) vs high (maize) 13C enrichment. To demonstrate the physiological value, we quantified exogenous vs total carbohydrate and fat oxidation continuously, in real time in mice varying in fat mass. Diet-induced obese mice were fed a single liquid mixed meal containing 13C-isotopic tracers of glucose or palmitate. Over 13 h, ~70% glucose and ~48% palmitate ingested were oxidised. Exogenous palmitate oxidation depended on body fat mass, which was not the case for exogenous glucose oxidation. We conclude that extending an InCa system with 13CO2 and 12CO2 sensors provides an accessible and powerful technique for real-time continuous quantification of exogenous and whole-body substrate oxidation in mouse models of human metabolic physiology.
Nanostructuring with Structural-Compositional Dual Heterogeneities Enhances Strength-Ductility Synergy in Eutectic High Entropy Alloy
Authors: Reddy S. R. , Yoshida S. , Bhattacharjee T. , Sake N. , Lozinko A. , Guo S. , Bhattacharjee P. P. , Tsuji N. 
Published: August 2019

AbstractA lamellar (L12 + B2) AlCoCrFeNi2.1 eutectic high entropy alloy (EHEA) was severely deformed by a novel hybrid-rolling process. During hybrid-rolling, the deformation was carried out in two stages, namely cryo-rolling followed by warm-rolling at 600 °C. The strain (ε) imparted in each of these steps was identical ~1.2, resulting in a total strain of ε~2.4 (corresponding to 90% reduction in thickness). The novel processing strategy resulted in an extremely heterogeneous microstructure consisting of retained lamellar and transformed nanocrystalline regions. Each of these regions consisted of different phases having different crystal structures and chemical compositions. The novel structure-composition dual heterogeneous microstructure originated from the stored energy of the cryo-rolling which accelerated transformations during subsequent low temperature warm-rolling. The dual heterogeneous microstructure yielded an unprecedented combination of strength (~2000 MPa) and ductility (~8%). The present study for the first time demonstrated that dual structure-composition heterogeneities can be a novel microstructural design strategy for achieving outstanding strength-ductility combination in multiphase high entropy alloys.
The waveguiding of sound using lines of resonant holes
Authors: Ward G. P. , Hibbins A. P. , Sambles J. R. , Smith J. D. 
Published: August 2019

AbstractThe dispersion of an acoustic surface wave supported by a line of regularly spaced, open ended holes in an acrylic plate, is characterised by precise measurement of its localised acoustic fields. We illustrate the robust character of this surface wave and show its potential for control of sound by the acoustic waveguiding provided by a ring of regularly spaced holes. A single line of open-ended holes is shown to act as simple acoustic waveguide that can be readily manipulated to control the flow of sound.
Biocompatibility between Silicon or Silicon Carbide surface and Neural Stem Cells
Authors: Bonaventura Gabriele , Iemmolo Rosario , La Cognata Valentina , Zimbone Massimo , La Via Francesco , Fragalà Maria Elena , Barcellona Maria Luisa , Pellitteri Rosalia , Cavallaro Sebastiano 
Published: August 2019

AbstractSilicon has been widely used as a material for microelectronic for more than 60 years, attracting considerable scientific interest as a promising tool for the manufacture of implantable medical devices in the context of neurodegenerative diseases. However, the use of such material involves responsibilities due to its toxicity, and researchers are pushing towards the generation of new classes of composite semiconductors, including the Silicon Carbide (3C-SiC). In the present work, we tested the biocompatibility of Silicon and 3C-SiC using an in vitro model of human neuronal stem cells derived from dental pulp (DP-NSCs) and mouse Olfactory Ensheathing Cells (OECs), a particular glial cell type showing stem cell characteristics. Specifically, we investigated the effects of 3C-SiC on neural cell morphology, viability and mitochondrial membrane potential. Data showed that both DP-NSCs and OECs, cultured on 3C-SiC, did not undergo consistent oxidative stress events and did not exhibit morphological modifications or adverse reactions in mitochondrial membrane potential. Our findings highlight the possibility to use Neural Stem Cells plated on 3C-SiC substrate as clinical tool for lesioned neural areas, paving the way for future perspectives in novel cell therapies for neuro-degenerated patients.
Interspecific competition among catch crops modifies vertical root biomass distribution and nitrate scavenging in soils
Authors: Heuermann Diana , Gentsch Norman , Boy Jens , Schweneker Dörte , Feuerstein Ulf , Groß Jonas , Bauer Bernhard , Guggenberger Georg , von Wirén Nicolaus 
Published: August 2019

AbstractThe potential of a plant species to acquire nutrients depends on its ability to explore the soil by its root system. Co-cultivation of different species is anticipated to lead to vertical root niche differentiation and thus to higher soil nutrient depletion. Using a qPCR-based method we quantified root biomass distribution of four catch crop species in vertical soil profiles in pure vs. mixed stands. Pure stands of mustard and phacelia robustly reached 70 cm soil depth, while oat preferably colonized upper soil layers, and clover developed the shallowest and smallest root system. Analysis of residual nitrate pools in different soil depths and correlation with root biomass showed that, besides rooting depth also root biomass determines soil nitrogen depletion. While occupying the same vertical niches as in pure stands, mustard and phacelia dominated total root biomass of the mix. In contrast, root biomass of clover and oat was severely suppressed in presence of the other species. Below-ground biomass profiling indicated low niche complementarity among the root systems of the examined species. Nonetheless, the mixture mostly overyielded root biomass of the pure stands and thus shows higher potential for efficient soil exploration by roots.
Cellulose dissolution in diallylimidazolium methoxyacetate + N-methylpyrrolidinone mixture
Authors: Xu Airong , Wang Yongxin , Liu Rukuan 
Published: August 2019

AbstractThe utilization of cellulose in industrial applicat is of great significance to sustainable development of human society and reducing dependence on dwindling fossil resources. Nevertheless, this utilization of cellulose has actually been limited due to its insolubilization. Here, novel solvents consisting of diallylimidazolium methoxy acetate ([A2im][CH3OCH2COO]) and N-methylpyrrolidinone (NMP) were developed. The solubility of cellulose in [A2im][CH3OCH2COO]/NMP was determined, and the influence of [A2im][CH3OCH2COO]/NMP molar ratio on cellulose dissolution was systematically investigated. Meanwhile, we also presented the affecting factors of the cellulose material fabrication including preparation approach, [A2im][CH3OCH2COO] and cellulose solution concentration. Attractively, the [A2im][CH3OCH2COO]/NMP solvents display much powerful dissolution capacity for cellulose even at 25 °C (25.4 g 100 g−1). This is mainly ascribed to the combined factors: The hydrogen bond interactions of the H2, H4 and H6 in [A2im]+ and carboxyl O atom in [CH3OCH2COO]− with the hydroxyl H atom and O atom in cellulose; the dissociation of NMP towards [A2im][CH3OCH2COO]; the stabilization of NMP towards the dissolved cellulose chains. In addition, the thermostability and chemical structure of the regenerated cellulose from the solvents was also estimated.
Atomic layer deposition of ZnO/TiO2 nanolaminates as ultra-long life anode material for lithium-ion batteries
Authors: Cao Yan-Qiang , Wang Shan-Shan , Liu Chang , Wu Di , Li Ai-Dong 
Published: August 2019

AbstractIn this work, we designed ZnO/TiO2 nanolaminates by atomic layer deposition (ALD) as anode material for lithium ion batteries. ZnO/TiO2 nanolaminates were fabricated on copper foil by depositing unit of 26 cycles ZnO/26 cycles TiO2 repeatedly using ALD. ZnO/TiO2 nanolaminates are much more stable than pristine ZnO films during electrochemical cycling process. Therefore, ZnO/TiO2 nanolaminates exhibit excellent lithium storage performance with an improved cycling performance and superior rate capability compared to pristine ZnO films. Moreover, coulombic efficiency (CE) of ZnO/TiO2 nanolaminates is above 99%, which is much higher than the value of pristine ZnO films. Excellent ultralong-life performance is gained for ZnO/TiO2 nanolaminates, retaining a reversible capacity of ~667 mAh g−1 within cut-off voltage of 0.05-2.5 V after 1200 cycles of charge-discharge at 500 mA g−1. Constructing nanolaminates structures via ALD might open up new opportunities for improving the performance of anode materials with large volume expansion in lithium ion batteries.
A quest for coordination among activities at the replisome
Authors: Kapadia Nitin , Reyes-Lamothe Rodrigo 
Published: August 2019

Abstract
Faithful DNA replication is required for transmission of the genetic material across generations. The basic mechanisms underlying this process are shared among all organisms: progressive unwinding of the long double-stranded DNA; synthesis of RNA primers; and synthesis of a new DNA chain. These activities are invariably performed by a multi-component machine called the replisome. A detailed description of this molecular machine has been achieved in prokaryotes and phages, with the replication processes in eukaryotes being comparatively less known. However, recent breakthroughs in the in vitro reconstitution of eukaryotic replisomes have resulted in valuable insight into their functions and mechanisms. In conjunction with the developments in eukaryotic replication, an emerging overall view of replisomes as dynamic protein ensembles is coming into fruition. The purpose of this review is to provide an overview of the recent insights into the dynamic nature of the bacterial replisome, revealed through single-molecule techniques, and to describe some aspects of the eukaryotic replisome under this framework. We primarily focus on Escherichia coli and Saccharomyces cerevisiae (budding yeast), since a significant amount of literature is available for these two model organisms. We end with a description of the methods of live-cell fluorescence microscopy for the characterization of replisome dynamics.
Recent updates on the molecular network of elastic fiber formation
Authors: Shin Seung Jae , Yanagisawa Hiromi 
Published: August 2019

Abstract
Elastic fibers confer elasticity and recoiling to tissues and organs and play an essential role in induction of biochemical responses in a cell against mechanical forces derived from the microenvironment. The core component of elastic fibers is elastin (ELN), which is secreted as the monomer tropoelastin from elastogenic cells, and undergoes self-aggregation, cross-linking and deposition on to microfibrils, and assemble into insoluble ELN polymers. For elastic fibers to form, a microfibril scaffold (primarily formed by fibrillin-1 (FBN1)) is required. Numerous elastic fiber-associated proteins are involved in each step of elastogenesis and they instruct and/or facilitate the elastogenesis processes. In this review, we designated five proteins as key molecules in elastic fiber formation, including ELN, FBN1, fibulin-4 (FBLN4), fibulin-5 (FBLN5), and latent TGFβ-binding protein-4 (LTBP4). ELN and FBN1 serve as building blocks for elastic fibers. FBLN5, FBLN4 and LTBP4 have been demonstrated to play crucial roles in elastogenesis through knockout studies in mice. Using these molecules as a platform and expanding the elastic fiber network through the generation of an interactome map, we provide a concise review of elastogenesis with a recent update as well as discuss various biological functions of elastic fiber-associated proteins beyond elastogenesis in vivo.
Stable photonic transmitting link based on passive phase compensation with active‐assisted control method
Authors: Zhu Xiang , Jin Tao , Fu Ying , Chi Hao , Zuo Lulu , Liu Weimin , Wang Qihang 
Published: August 2019

The authors demonstrate a photonic link for stable radio frequency (RF) dissemination over fibre based on passive phase compensation with an active‐assisted control method. A variable optical delay line is introduced in the transmitting link for active‐assisted phase compensation. After active‐assisted compensation, the residual jitter is <500 fs. Experimentally, an RF signal at a frequency of 5 GHz is successfully transmitted over a 20‐km fibre link, with the stability of 2.49 × 10−14 at 1 s and 9.19 × 10−18 at 104 s.
Gas versus dust sizes of protoplanetary discs: effects of dust evolution
Authors: Trapman L. , Facchini S. , Hogerheijde M. R. , van Dishoeck E. F. , Bruderer S. 
Published: August 2019

Context. The extent of the gas in protoplanetary discs is observed to be universally larger than the extent of the dust. This is often attributed to radial drift and grain growth of the millimetre grains, but line optical depth produces a similar observational signature.
Aims. We investigate in which parts of the disc structure parameter space dust evolution and line optical depth are the dominant drivers of the observed gas and dust size difference.
Methods. Using the thermochemical model DALI with dust evolution included we ran a grid of models aimed at reproducing the observed gas and dust size dichotomy.
Results. The relation between Rdust and dust evolution is non-monotonic and depends on the disc structure. The quantity Rgas is directly related to the radius where the CO column density drops below 1015 cm−2 and CO becomes photodissociated; Rgas is not affected by dust evolution but scales with the total CO content of the disc. While these cases are rare in current observations, Rgas/Rdust > 4 is a clear sign of dust evolution and radial drift in discs. For discs with a smaller Rgas/Rdust, identifying dust evolution from Rgas/Rdust requires modelling the disc structure including the total CO content. To minimize the uncertainties due to observational factors requires FWHMbeam < 1× the characteristic radius and a peak S/N > 10 on the 12CO emission moment zero map. For the dust outer radius to enclose most of the disc mass, it should be defined using a high fraction (90–95%) of the total flux. For the gas, any radius enclosing >60% of the 12CO flux contains most of the disc mass.
Conclusions. To distinguish radial drift and grain growth from line optical depth effects based on size ratios requires discs to be observed at high enough angular resolution and the disc structure should to be modelled to account for the total CO content of the disc.
Magnetohydrodynamic waves in braided magnetic fields
Authors: Howson T. A. , De Moortel I. , Reid J. , Hood A. W. 
Published: August 2019

Aims. We investigate the propagation of transverse magnetohydrodynamic (MHD) wave fronts through a coronal plasma containing a braided magnetic field.
Methods. We performed a series of three dimensional MHD simulations in which a small amplitude, transverse velocity perturbation is introduced into a complex magnetic field. We analysed the deformation of the wave fronts as the perturbation propagates through the braided magnetic structures and explore the nature of Alfvénic wave phase mixing in this regime. We considered the effects of viscous dissipation in a weakly non-ideal plasma and evaluate the effects of field complexity on wave energy dissipation.
Results. Spatial gradients in the local Alfvén speed and variations in the length of magnetic field lines ensure that small scales form throughout the propagating wave front due to phase mixing. Additionally, the presence of complex, intricate current sheets associated with the background field locally modifies the polarisation of the wave front. The combination of these two effects enhances the rate of viscous dissipation, particularly in more complex field configurations. Unlike in classical phase mixing configurations, the greater spatial extent of Alfvén speed gradients ensures that wave energy is deposited over a larger cross-section of the magnetic structure. Further, the complexity of the background magnetic field ensures that small gradients in a wave driver can map to large gradients within the coronal plasma.
Conclusions. The phase mixing of transverse MHD waves in a complex magnetic field will progress throughout the braided volume. As a result, in a non-ideal regime wave energy will be dissipated over a greater cross-section than in classical phase mixing models. The formation rate of small spatial scales in a propagating wave front is a function of the complexity of the background magnetic field. As such, if the coronal field is sufficiently complex it remains plausible that phase mixing induced wave heating can contribute to maintaining the observed temperatures. Furthermore, the weak compressibility of the transverse wave and the observed phase mixing pattern may provide seismological information about the nature of the background plasma.
Variations in the Morphology of Human Lungs and its Clinical Implications
Authors: Jacob Suja Mary , Venniyoor Vaibhav , Pillay Minnie 
Published: August 2019

Objective To observe variations in the fissures, in the lobes, and in the hilar pattern of lungs and correlate these findings with clinical implications.
Materials and Methods The present study was performed on random lung specimens available in the Department of Anatomy. A total of 96 lungs (47 right and 49 left) were studied for variations in the fissures and lobes, and 92 lungs (45 right and 47 left) for variations in the hilar pattern.
Results Among the right-sided lungs, 70% presented incomplete horizontal fissure, 15% with absence of the horizontal fissure, and 51% with incomplete oblique fissure. Accessory fissures were also seen, but incomplete, and accounted for 17% of the total number of right lungs.Among the left-sided lungs, 62% presented incomplete oblique fissures, and 4% with absence of the oblique fissure. Accessory fissures accounted for 6% of the total number of left lungs.Regarding hilar pattern variations, 11% of the right-sided lungs showed > 2 bronchi, 69% showed > 2 pulmonary veins, and 37% showed > 1 pulmonary artery.Among the left-sided lungs, 57% showed > 1 bronchi, 21% showed > 2 pulmonary veins, and 17% showed > 1 pulmonary artery.
Conclusion The field of pulmonary surgery is now highly advanced, with well-developed radiological and endoscopic techniques. Hence, a proper understanding and knowledge of these morphological variations of lung fissures and of the hilar pattern would be advantageous for surgeons, as well as for radiologists.
Morfologia Glenoidea En La Artrosis Glenohumeral Primaria: Factores Preoperatorios Relevantes
Authors: Moran Nicolás , Arias Valentina , Lazo José , Melean Patricio 
Published: August 2019

ResumenEl objetivo primario de esta revisión es realizar una actualización de los conceptos básicos relacionados a los cambios morfológicos glenoideos durante la artrosis glenohumeral primaria, métodos de medición relevantes y describir las modificaciones en la nueva clasificación de Gilles Walch.La medición de esos parámetros influyen en forma directa tanto en el acto quirúrgico dentro una prótesis de hombro como en los resultados clínicos post operatorios.Los diferentes métodos de medición descritos en la literatura han permitido evaluar la magnitud de esos fenómenos morfológicos y describir la “glenoides bicóncava”, caracterizada por presentar 3 subtipos: la paleoglena, neoglena y la glena intermedia.A partir de esos conceptos, Walch en 1999 describe la clasificación de los cambios morfológicos glenoideos en artrosis primaria, la cual ha presentado una reciente modificación debido a la mala concordancia intra e interobservador de esa medición obtenida por varios investigadores, además de reconocer una carencia en la precisión para describir cada subtipo.A la fecha, diferentes autores investigan la influencia de esos factores morfológicos preoperatorios en los resultados post operatorios, y hasta que valor límite podrían guiar un tratamiento especifico.
Conclusión Enfatizamos que un análisis acabado y minucioso de la morfología glenoidea es importante para una adecuada planificación quirúrgica en artroplastia de hombro, ya que eso puede guiarnos en cuál técnica o implante puede ser el más adecuado para cada tipo de glenoides.
Effectiveness of Using Photographs of the Change in Standing Posture on Postoperative Patient-Reported Satisfaction and Quality of Life
Authors: Misir Abdulhamit , Kizkapan Turan Bilge , Tas Suleyman Kasim , Yildiz Kadir Ilker , Uzun Erdal , Ozcamdalli Mustafa 
Published: August 2019

AbstractDissatisfaction is still an important problem in a small but important group of patients who undergo total knee arthroplasty. This study was designed to evaluate the effectiveness of showing patients the change in their standing posture, before and after total knee replacement, using standing photographs (anterior, posterior, and lateral view), on improving self-reported quality of life and satisfaction. Full-length lower extremity radiographs and standing photographs were obtained prior to total knee replacement and 6 months after surgery in the study group. In the control group, radiographs and photographs were not obtained. The hip–knee–ankle angle and mechanical axis deviation were compared between the two limbs and two groups. The changes in the following outcome measures were evaluated from baseline to 6 months after surgery: Short Form-36 Survey, Knee Injury and Osteoarthritis Outcome Score, Western Ontario and McMaster Universities Osteoarthritis Index, Oxford Knee Score, and Knee Society score. Postoperative measures were also compared before and after showing patients their standing photographs, and between two groups. The study and control groups included 71 and 44 patients, respectively, with unilateral or bilateral knee osteoarthritis (Kellgren–Lawrence Grade 3 or 4), with a mean age of 66 years. All patients had a varus malalignment, with significant improvement in postoperative limb alignment (p < 0.001). All self-reported outcome measures improved after surgery (p < 0.05), with the exception of mental health in the Short Form-36. Self-reported scores further improved after patients seeing their standing photographs (p < 0.05), with the exception of mental health and social role functioning. There were also significantly improved scores in the study group than control group after showing photographs (p < 0.05). Showing patients the pre-to-postoperative change in their standing posture might be an easy-to-administer method to improve patient satisfaction with the outcome of total knee replacement and self-reported quality of life. The Level of Evidence for this study is four.