bluuebunny/crossref_metadata_2025_split huggingface dataset:
SILAC–based quantitative MS approach for real-time recording protein-mediated cell-cell interactions
Authors: Wang Xixi , He Yu , Ye Yang , Zhao Xinyu , Deng Shi , He Gu , Zhu Hongxia , Xu Ningzhi , Liang Shufang 
Published: May 2018

AbstractIn tumor microenvironment, interactions among multiple cell types are critical for cancer progression. To understand the molecular mechanisms of these complex interplays, the secreted protein analysis between malignant cancer cells and the surrounding nonmalignant stroma is a good viewpoint to investigate cell-cell interactions. Here, we developed two stable isotope labeling of amino acids in cell culture (SILAC)-based mass spectrometry (MS)/MS approaches termed spike-in SILAC and triple-SILAC to quantify changes of protein secretion level in a cell co-cultured system. Within the co-culture system of CT26 and Ana-1 cells, the spike-in SILAC and triple-SILAC MS approaches are sensitive to quantitatively measure protein secretion changes. Three representative quantified proteins (Galectin-1, Cathepsin L1 and Thrombospondin-1) by two SILAC-based MS methods were further validated by Western blotting, and the coming result matched well with SILACs’. We further applied these two SILACs to human cell lines, NCM460 and HT29 co-culture system, for evaluating the feasibility, which confirmed the spike-in and triple SILAC were capable of monitoring the changed secreted proteins of human cell lines. Considering these two strategies in time consuming, sample complexity and proteome coverage, the triple-SILAC way shows more efficiency and economy for real-time recording secreted protein levels in tumor microenvironment.
Unravelling miRNA regulation in yield of rice (Oryza sativa) based on differential network model
Authors: Hu Jihong , Zeng Tao , Xia Qiongmei , Qian Qian , Yang Congdang , Ding Yi , Chen Luonan , Wang Wen 
Published: May 2018

AbstractRice (Oryza sativa L.) is one of the essential staple food crops and tillering, panicle branching and grain filling are three important traits determining the grain yield. Although miRNAs have been reported being regulating yield, no study has systematically investigated how miRNAs differentially function in high and low yield rice, in particular at a network level. This abundance of data from high-throughput sequencing provides an effective solution for systematic identification of regulatory miRNAs using developed algorithms in plants. We here present a novel algorithm, Gene Co-expression Network differential edge-like transformation (GRN-DET), which can identify key regulatory miRNAs in plant development. Based on the small RNA and RNA-seq data, miRNA-gene-TF co-regulation networks were constructed for yield of rice. Using GRN-DET, the key regulatory miRNAs for rice yield were characterized by the differential expression variances of miRNAs and co-variances of miRNA-mRNA, including osa-miR171 and osa-miR1432. Phytohormone cross-talks (auxin and brassinosteroid) were also revealed by these co-expression networks for the yield of rice.
Fission Yeast Sirtuin Hst4 Functions in Preserving Genomic Integrity by Regulating Replisome Component Mcl1
Authors: Konada Lahiri , Aricthota Shalini , Vadla Raghavendra , Haldar Devyani 
Published: May 2018

AbstractThe Schizosaccharomyces pombe sirtuin Hst4, functions in the maintenance of genome stability by regulating histone H3 lysine56 acetylation (H3K56ac) and promoting cell survival during replicative stress. However, its molecular function in DNA damage survival is unclear. Here, we show that hst4 deficiency in the fission yeast causes S phase delay and DNA synthesis defects. We identified a novel functional link between hst4 and the replisome component mcl1 in a suppressor screen aimed to identify genes that could restore the slow growth and Methyl methanesulphonate (MMS) sensitivity phenotypes of the hst4Δ mutant. Expression of the replisome component Mcl1 rescues hst4Δ phenotypes. Interestingly, hst4 and mcl1 show an epistatic interaction and suppression of hst4Δ phenotypes by mcl1 is H3K56 acetylation dependent. Furthermore, Hst4 was found to regulate the expression of mcl1. Finally, we show that hSIRT2 depletion results in decreased levels of And-1 (human orthologue of Mcl1), establishing the conservation of this mechanism. Moreover, on induction of replication stress (MMS treatment), Mcl1 levels decrease upon Hst4 down regulation. Our results identify a novel function of Hst4 in regulation of DNA replication that is dependent on H3K56 acetylation. Both SIRT2 and And-1 are deregulated in cancers. Therefore, these findings could be of therapeutic importance in future.
Interaction of hepatic stellate cells with neutrophils and macrophages in the liver following oncogenic kras activation in transgenic zebrafish
Authors: Yang Qiqi , Yan Chuan , Gong Zhiyuan 
Published: May 2018

AbstractActivation of hepatic stellate cells (HSC) plays a crucial role in the liver disease progression from liver fibrosis/cirrhosis to cancer. Here, we found a rapid change of microenvironment after kras
V12
-induction in zebrafish liver with progressively increased stromal cell number and enlarged liver size. Neutrophils and macrophages exhibited a faster response than HSCs. By manipulating the numbers of neutrophils and macrophages through morpholino knockdown, we found that macrophages contributed to both HSC survival and activation while neutrophils appear to be only required for HSC activation. Serotonin, which is essential for HSC survival and activation, was found up-regulated in hepatocytes and macrophages, but not in neutrophils after kras
V12
induction. Serotonin receptor was highly expressed in HSCs; increase of the receptor activity by an agonist stimulated HSCs and oncogenic growth of the liver while an opposite effect was observed with an antagonist. Activated HSCs promoted the pro-tumorigenesis functions of neutrophils and macrophages through secretion of Tgfb1. Overall, these observations elucidated a cellular interaction in microenvironment where that upregulated serotonin in hepatocytes and macrophages activated HSCs. Since the microenvironment crosstalk plays a vital role in manipulation of liver carcinogenesis, the underlying mechanism may provide potential therapeutic targets for liver diseases.
Modulations in martensitic Heusler alloys originate from nanotwin ordering
Authors: Gruner M. E. , Niemann R. , Entel P. , Pentcheva R. , Rößler U. K. , Nielsch K. , Fähler S. 
Published: May 2018

AbstractHeusler alloys exhibiting magnetic and martensitic transitions enable applications like magnetocaloric refrigeration and actuation based on the magnetic shape memory effect. Their outstanding functional properties depend on low hysteresis losses and low actuation fields. These are only achieved if the atomic positions deviate from a tetragonal lattice by periodic displacements. The origin of the so-called modulated structures is the subject of much controversy: They are either explained by phonon softening or adaptive nanotwinning. Here we used large-scale density functional theory calculations on the Ni2MnGa prototype system to demonstrate interaction energy between twin boundaries. Minimizing the interaction energy resulted in the experimentally observed ordered modulations at the atomic scale, it explained that a/b twin boundaries are stacking faults at the mesoscale, and contributed to the macroscopic hysteresis losses. Furthermore, we found that phonon softening paves the transformation path towards the nanotwinned martensite state. This unified both opposing concepts to explain modulated martensite.
Effect of different types of olive oil pomace dietary supplementation on the rumen microbial community profile in Comisana ewes
Authors: Mannelli Federica , Cappucci Alice , Pini Francesco , Pastorelli Roberta , Decorosi Francesca , Giovannetti Luciana , Mele Marcello , Minieri Sara , Conte Giuseppe , Pauselli Mariano , Rapaccini Stefano , Viti Carlo , Buccioni Arianna 
Published: May 2018

AbstractOlive oil pomace (OOP) is a bio-waste rich in highly soluble polyphenols. OOP has been proposed as an additive in ruminant feeding to modulate rumen fermentations. Three groups of ewes were fed the following different diets: a control diet and two diets supplemented with OOP, obtained with a two-phase (OOP2) or three-phase (OOP3) olive milling process. Rumen liquor (RL) showed a higher content of 18:3 cis9 cis12 cis15 (α-linolenic acid, α-LNA) with OOP2 inclusion, and of 18:2 cis9 trans11 (rumenic acid, RA) with OOP3 inclusion. The overall composition of the RL microbiota did not differ among treatments. Significant differences, between control and treated groups, were found for six bacterial taxa. In particular, RL microbiota from animals fed OOPs showed a reduction in Anaerovibrio, a lipase-producing bacterium. The decrease in the Anaerovibrio genus may lead to a reduction in lipolysis, thus lowering the amount of polyunsaturated fatty acids available for biohydrogenation. Milk from animals fed OOP showed a higher content of 18:1 cis9 (oleic acid, OA) but the α-LNA concentration was increased in milk from animals treated with OOP2 only. Therefore, inclusion of OOP in ruminant diets may be a tool to ameliorate the nutritional characteristics of milk.
Clinical and epidemiological features of Heart-Hand Syndrome: a hospital-based study in China
Authors: Yin Yaobin , Ji Jianguang , Borné Yan , Wang Yanqing , Zhao Junhui , Chen Shanlin , Tian Wen 
Published: May 2018

AbstractHeart–hand syndrome (HHS) is a clinically and genetically heterogeneous disorder characterized by the co-occurrence of a congenital cardiac disease and an upper limb malformation. This study revealed the clinical and epidemiological features of HHS in China. The study was based on patients with congenital upper limb malformation treated in Beijing Ji Shui Tan hospital from October 1st, 2013 to October 1st, 2016. We reviewed the patients’ medical records and identified patients with abnormal ultrasonic cardiogram and/or electrocardiogram (ECG). A total of 1462 patients (910 male and 552 female) were identified to be treated for congenital upper limb malformation. Among them, 172 (11.8%) had abnormal ultrasonic cardiogram and/or ECG. Abnormal heart structure were discovered in 121 patients and 51 patients had abnormal ECG. The most common type of abnormal heart structure was tricuspid regurgitation (53/121, 43.8%), while the most common abnormal ECG was wave patterns (22/51, 43.1%). This hospital-based study suggests that the rate of congenital heart disease is high in patients treated for congenital upper extremity malformation in China. Surgeons and anesthetists should be aware of the comorbidity and preoperational examination of congenital heart diseases is highly needed to avoid complications during operation.
Laser-patterned metallic interconnections for all stretchable organic electrochemical transistors
Authors: Marchiori Bastien , Delattre Roger , Hannah Stuart , Blayac Sylvain , Ramuz Marc 
Published: May 2018

AbstractWe describe a process allowing the patterning of fully stretchable organic electrochemical transistors (OECTs). The device consists of an active stretchable area connected with stretchable metallic interconnections. The current literature does not provide a complete, simple and accurate process using the standard thin film microelectronic techniques allowing the creation of such sensors. An innovative patterning process based on the combination of laser ablation and thermal release tape ensures the fabrication of highly stretchable metallic lines – encapsulated in polydimethylsiloxane – from conventional aluminium tape. State-of-the-art stretchability up to 70% combined with ultra-low mOhms resistance is demonstrated. We present a photolithographic process to pattern the organic active area onto stretchable substrate. Finally the formulation of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) is tuned to achieve an OECT with a maximum stretchability of 38% while maintaining transconductance up to 0.35 mS and channel current as high as 0.2 mA.
Direct phenotypic conversion of human fibroblasts into functional osteoblasts triggered by a blockade of the transforming growth factor-β signal
Authors: Yamamoto Kenta , Kishida Tsunao , Nakai Kei , Sato Yoshiki , Kotani Shin-ichiro , Nishizawa Yuta , Yamamoto Toshiro , Kanamura Narisato , Mazda Osam 
Published: May 2018

AbstractA procedure to generate functional osteoblasts from human somatic cells may pave the way to a novel and effective transplantation therapy in bone disorders. Here, we report that human fibroblasts were induced to show osteoblast phenotypes by culturing with ALK5 i II, which is a specific inhibitor for activin-like kinase 5 (ALK5) (tumor growth factor-β receptor 1 (TGF-β R1)). Cells cultured with ALK5 i II expressed osteoblast-specific genes and massively produced calcified bone matrix, similar to the osteoblasts induced from mesenchymal stem cells (MSC-OBs). Treatment with vitamin D3 in addition to ALK5 i II induced more osteoblast-like characters, and the efficiency of the conversion reached approximately 90%. The chemical compound-mediated directly converted osteoblasts (cOBs) were similar to human primary osteoblasts in terms of expression profiles of osteoblast-related genes. The cOBs abundantly produced bone matrix in vivo and facilitated bone healing after they were transplanted into immunodeficient mice at an artificially induced defect lesion in femoral bone. The present procedure realizes a highly efficient direct conversion of human fibroblasts into transgene-free and highly functional osteoblasts, which might be applied in a novel strategy of bone regeneration therapy in bone diseases.
Wideband performance analysis of ground-plane cloak designed with polarization-independent randomly patterned metamaterial
Authors: Lee Hyunsoo , Koh Il-Suek , Kim Yongjune , Lee Yongshik 
Published: May 2018

AbstractA ground-plane cloak is designed based on the quasi-conformal mapping method to hide a perfectly conducting object. It is fabricated with a metamaterial, a mixture of a dielectric and air. Using the dielectric mixing formula, the required volume fraction is calculated for a designed refractive index of the cloak. To guarantee the statistical isotropy of the cloak structure, many small pixels are randomly connected to form the metamaterial. A three-dimensional printing machine is used to implement the whole designed cloak structure. The performance of the cloak is experimentally analyzed over a wide frequency range for both independent polarizations. The measurement is also validated by numerical full-wave simulations. Because the quasi-conformal mapping generates unrealistic refractive indices, less than unity, those are removed. The effect of the truncation is experimentally observed and theoretically analyzed by the ray-tracing method.
Variation in faecal microbiota in a group of horses managed at pasture over a 12-month period
Authors: Salem Shebl E. , Maddox Thomas W. , Berg Adam , Antczak Philipp , Ketley Julian M. , Williams Nicola J. , Archer Debra C. 
Published: May 2018

AbstractColic (abdominal pain) is a common cause of mortality in horses. Change in management of horses is associated with increased colic risk and seasonal patterns of increased risk have been identified. Shifts in gut microbiota composition in response to management change have been proposed as one potential underlying mechanism for colic. However, the intestinal microbiota in normal horses and how this varies over different seasons has not previously been investigated. In this study the faecal microbiota composition was studied over 12 months in a population of horses managed at pasture with minimal changes in management. We hypothesised that gut microbiota would be stable in this population over time. Faecal samples were collected every 14 days from 7 horses for 52 weeks and the faecal microbiota was characterised by next-generation sequencing of 16S rRNA genes. The faecal microbiota was dominated by members of the phylum Firmicutes and Bacteroidetes throughout. Season, supplementary forage and ambient weather conditions were significantly associated with change in the faecal microbiota composition. These results provide important baseline information demonstrating physiologic variation in the faecal microbiota of normal horses over a 12-month period without development of colic.
Clay-induced DNA breaks as a path for genetic diversity, antibiotic resistance, and asbestos carcinogenesis
Authors: González-Tortuero Enrique , Rodríguez-Beltrán Jerónimo , Radek Renate , Blázquez Jesús , Rodríguez-Rojas Alexandro 
Published: May 2018

AbstractNatural clays and synthetic nanofibres can have a severe impact on human health. After several decades of research, the molecular mechanism of how asbestos induces cancer is not well understood. Different fibres, including asbestos, can penetrate cell membranes and introduce foreign DNA in bacterial and eukaryotic cells. Incubating Escherichia coli under friction forces with sepiolite, a clayey material, or with asbestos, causes double-strand DNA breaks. Antibiotics and clays are used together in animal husbandry, the mutagenic effect of these fibres could be a pathway to antibiotic resistance due to the friction provided by peristalsis of the gut from farm animals in addition to horizontal gene transfer. Moreover, we raise the possibility that the same mechanism could generate bacteria diversity in natural scenarios, playing a role in the evolution of species. Finally, we provide a new model on how asbestos may promote mutagenesis and cancer based on the observed mechanical genotoxicity.
High‐efficiency circular dense dielectric patch antenna with frequency selectivity
Authors: Xu Kai , Shi Jin 
Published: May 2018

A circular dense dielectric (DD) patch antenna realised by stacking an upper DD patch on the lower DD patch is proposed to achieve high efficiency and frequency selectivity. The high efficiency is attributed to the non‐metallic radiators and the radiations of both lower and upper DD patches operating at TM11 mode. To achieve higher frequency selectivity than that of the reported DD patch antennas, the opposite E‐field distribution inside the upper DD patch and the quarter‐wavelength open‐circuit stub produce two radiation nulls near the lower band‐edge, while the opposite E‐field distribution inside the lower DD patch and the opposite E‐field distribution at the edges of the two DD patches produce two radiation nulls near the upper band‐edge. For demonstration, a prototype covering the fifth generation (5G) band (3.4–3.6 GHz) achieves the simulated radiation efficiency of 96%, the measured peak gain of 9.2 dBi, 10 dB impedance bandwidth of 12.3%, four radiation nulls, and the cross‐polarisation rejection larger than 22 dB within the 3‐dB beam range at the centre frequency. The results show the features of high efficiency, multiple radiation nulls, wide bandwidth and high gain simultaneously while the reported DD patch antennas just have part of the features.
Single‐ended travelling wave protection algorithm based on full waveform in the time and frequency domains
Authors: Deng Feng , Li Xinran , Zeng Xiangjun 
Published: May 2018

Single‐ended travelling‐wave‐based protection has been available for several years, providing the advantages of low cost and no requirement for communication and synchronisation with the remote end. However, conventional single‐ended travelling‐wave‐based protection has low reliability due to its use of only partial fault information, and it has high dependence on extracting a second reflected wavefront. A new single‐ended travelling‐wave‐based protection algorithm using the 2 ms full‐waveform post‐fault signal in the time–frequency domain, which avoids the discrimination of the reflected wavefront, is presented. First, the full‐waveform representation of the travelling wave in 3D subspace is proposed. The fault characteristics of the wideband travelling wave are analysed at different scales. Second, for faults that occur within one line or on adjacent lines, the propagation characteristics and reflection and refraction process are analysed in detail. Then, the correlation and difference of the full waveform are presented qualitatively and quantitatively. Finally, a time–frequency spectrum matrix is established based on the full waveform, and the protection algorithm is developed by using a time–frequency spectrum matrix and wave matching technology. Extensive simulations under different conditions verify the wide applicability and high reliability of the proposed algorithm.
Hemostatic powder application for control of acute upper gastrointestinal bleeding in patients with gastric malignancy
Authors: Kim Yeong , Park Jun , Kim Eun , Shin Sung , Lee Sang , Lee Young 
Published: May 2018

Abstract
Background and study aims Despite use of various endoscopic therapies in patients with tumor-related bleeding, various rates of successful immediate hemostasis and short-term rebleeding have been reported. We aimed to evaluate preliminary results of use of the EndoClot polysaccharide hemostatic system (PHS) in patients with acute upper gastrointestinal bleeding (UGIB) from a gastric malignancy.
Patients and methods We retrospectively analyzed data from a prospectively collected database of 12 patients with acute UGIB from a gastric malignancy who had been treated with EndoClot PHS. The EndoClot air compressor was used to propel 2 g of absorbable modified polymer particles onto the bleeding site. We checked successful immediate hemostasis, rebleeding events and mortality within 30 days.
Results Twelve patients were enrolled in the study. The median patient age was 72.5 years (range, 57 – 89). The initial median hemoglobin level was 6.75 g/dL (range, 5.4 – 8.9). The median systolic blood pressure was 114 mmHg (range, 86 – 153). Eleven patients had advanced gastric cancer and one patient had a gastrointestinal stromal tumor. The lower body of the stomach was the most common tumor location (58.3 %), and the median tumor size was 40 mm (range, 15 – 100). Immediate hemostasis was achieved in all patients, and rebleeding developed in 2 of 12 patients (16 %), 3 and 5 days after treatment. There were no significant Endoclot PHS-related adverse events, and there was no all-cause mortality at 30 days after the intervention.
Conclusions Endoclot PHS represents a new alternative, complementary, and promising therapy for patients with an acute UGIB from a gastric malignancy.