bluuebunny/crossref_metadata_2025_split huggingface dataset:
Towards comprehensive characterization of CRISPR-linked genes
Authors: Shmakov Sergey A. , Makarova Kira S. , Wolf Yuri I. , Severinov Konstantin V. , Koonin Eugene V. 
Published: February 2018

ABSTRACTThe CRISPR-Cas systems of bacterial and archaeal adaptive immunity consist of arrays of direct repeats separated by unique spacers and multiple CRISPR-associated (cas) genes encoding proteins that mediate the adaptation, CRISPR RNA maturation and interference stages of the CRISPR response. In addition to the relatively small set of core cas genes that are typically present in all representatives of each (sub)type of CRISPR-Cas systems and are essential for the defense function, numerous genes occur in CRISPR-cas loci only sporadically. Some of these have been shown to perform various ancillary roles in CRISPR response whereas the functional relevance of many others, if any, remains obscure. We developed a computational strategy for systematically detecting genes that are likely to be functionally linked to CRISPR-Cas systems. The approach is based on a “CRISPRicity” metric that measures the strength of CRISPR association for all protein-coding genes from sequenced bacterial and archaeal genomes. Uncharacterized genes with CRISPRicity values comparable to those of known cas genes are considered candidate CRISPR-ancillary genes, and we describe additional criteria to identify functionally relevant genes in the candidate set. About 80 genes that were not previously reported to be associated with CRISPR-Cas were identified as probable CRISPR-ancillary genes. A substantial majority of these genes reside in type III CRISPR-cas loci which implies exceptional functional versatility of type III systems. Numerous candidate CRISPR-ancillary genes encode integral membrane proteins suggestive of tight membrane connections of type III CRISPR-Cas whereas many other candidates are proteins implicated in various signal transduction pathways. These predictions provide ample material for improving annotation of CRISPR-cas loci and experimental characterization of previously unsuspected aspects of CRISPR-Cas functionality.SIGNIFICANCEThe CRISPR-Cas systems that mediate adaptive immunity in bacteria and archaea encompass a small set of core cas genes that are essential in a broad range of CRISPR-Cas systems. However, a much greater number of genes only sporadically co-occur with CRISPR-Cas, and for most of these, involvement in CRISPR-Cas functions has not been demonstrated. We developed a computational strategy that provides for systematic identification of CRISPR-linked proteins and prediction of their functional association with CRISPR-Cas systems. About 80 previously undetected, putative CRISPR-accessory proteins were identified. A large fraction of these proteins are predicted to be membrane-associated revealing an unknown side of CRISPR biology.
Long-term depression is independent of GluN2 subunit composition
Authors: Wong Jonathan M. , Gray John A. 
Published: February 2018

AbstractNMDA receptors (NMDARs) mediate major forms of both long-term potentiation (LTP) and long-term depression (LTD) and understanding how a single receptor can initiate both phenomena remains a major question in neuroscience. A prominent hypothesis implicates the NMDAR subunit composition, specifically GluN2A and GluN2B, in dictating the rules of synaptic plasticity. However, studies testing this hypotheses have yielded inconsistent and often contradictory results, especially for LTD. These inconsistent results may be due to challenges in the interpretation of subunit-selective pharmacology and in dissecting out the contributions of differential channel properties versus the interacting proteins unique to GluN2A or GluN2B. In this study, we address the pharmacological and biochemical challenges by utilizing a single-neuron genetic approach to delete NMDAR subunits in both male and female conditional knock-out mice. In addition, emerging evidence that non-ionotropic signaling through the NMDAR is sufficient for NMDAR-dependent LTD allowed the rigorous assessment of unique subunit contributions to NMDAR-dependent LTD while eliminating the variable of differential charge transfer. Here we find that neither the GluN2A nor the GluN2B subunit is strictly necessary for either non-ionotropic or ionotropic LTD.
A Kaposi’s Sarcoma-Associated Herpesvirus Infection Mechanism is Independent of Integrins α3β1, αVβ3, and αVβ5
Authors: TerBush Allison Alwan , Hafkamp Florianne , Lee Hee Jun , Coscoy Laurent 
Published: February 2018

ABSTRACTHost receptor usage by KSHV has been best studied using primary microvascular endothelial and fibroblast cells, although the virus infects a wide variety of cell types in culture and in natural infections. In these two infection models, KSHV adheres to the cell though heparan sulfate (HS) binding, then interacts with a complex of EphA2, xct, and integrins α3β1, αVβ3, αVβ5 to catalyze viral entry. We dissected this receptor complex at the genetic level with CRISPR-Cas9 to precisely determine receptor usage in two epithelial cell lines. Surprisingly, we discovered an infection mechanism that requires HS and EphA2 but is independent of αV- and β1-family integrin expression. Furthermore, infection appears to be independent of the EphA2 intracellular domain. We also demonstrated while two other endogenous Eph receptors were dispensable for KSHV infection, transduced EphA4 and EphA5 significantly enhanced infection of cells lacking EphA2.IMPORTANCEOur data reveals an integrin-independent route of KSHV infection and suggests that multiple Eph receptors besides EphA2 can promote and regulate infection. Since integrins and Eph receptors are large protein families with diverse expression patterns across cells and tissues, we propose that KSHV may engage with several proteins from both families in different combinations to negotiate successful entry into diverse cell types.
Imperfect information and participation in insurance markets: evidence from Italy
Authors: Santeramo Fabio Gaetano 
Published: February 2018

Purpose
The purpose of this paper is to investigate how experience in insurance contracts may influence participation in the Italian crop insurance market.

Design/methodology/approach
From Italian farm-level data, the author estimates a dynamic discrete choice model of participation to investigate the role of experience. The methodology, coupled with exploratory analysis of the data, allows one to compare the relevance of different sources of experience in the crop insurance decision-making process.

Findings
The author found that experience tends to be a catalyst for insurance participation. Policy implications are discussed, in particular, the author discusses on the importance of bolstering uptake to exploit the advantages of the inertia that emerge from experience, and the importance of initiatives to increase the knowledge of crop insurance instruments.

Originality/value
To the best of the author’s knowledge, the role of experience has been underinvestigated. The analysis has the specific contribution of modeling the potential role of experience (exploited after buying an insurance contract) on uptake in crop insurance programs.
Building capacity on ecosystem-based adaptation strategy to cope with extreme events and sea-level rise on the Uruguayan coast
Authors: Carro Inti , Seijo Leonardo , Nagy Gustavo J. , Lagos Ximena , Gutiérrez Ofelia 
Published: February 2018

Purpose
This study aims to show a case study of ecosystem-based adaptation (EbA) measures to increase coastal system’s resilience to extreme weather events and sea-level rise (SLR) implemented at Kiyú (Uruguayan coast of the Rio de la Plata river estuary).

Design/methodology/approach
A participatory process involving the community and institutional stakeholders was carried out to select and prioritise adaptation measures to reduce the erosion of sandy beaches, dunes and bluffs due to extreme wind storm surge and rainfall, SLR and mismanagement practices. The recovery of coastal ecosystems was implemented through soft measures (green infrastructure) such as revegetation with native species, dune regeneration, sustainable drainage systems and the reduction of use pressures.

Findings
Main achievements of this case study include capacity building of municipal staff and stakeholders, knowledge exchanges with national-level decision makers and scientists and the incorporation of EbA approaches by subnational-level coastal governments. To consolidate EbA, the local government introduced innovations in the coastal management institutional structure.

Originality/value
The outcomes of the article include, besides the increase in the resilience of social-ecological systems, the strengthening of socio-institutional behaviour, structure and sustainability. This experience provides insights for developing a strategy for both Integrated Coastal Management and climate adaptation at the national scale.
Customer participation in services and employee innovative behavior
Authors: Li Minglong , Hsu Cathy H.C. 
Published: February 2018

PurposeThis paper aims to investigate the influence of customer participation in services on the innovative behaviors of employees. Although previous studies have acknowledged the importance of customers in service innovation and investigated how customer participation in product development teams affect innovation, the effect of mandatory customer participation in services on the employee innovative behavior has not been examined. In addition to addressing such gap, this study proposed the mediating role of interpersonal trust in the relationship between customer participation and employee innovative behavior and then tested the hypotheses in a restaurant context.Design/methodology/approachA total of 514 valid questionnaires were collected from frontline employees or entry-level managers in 25 well-known restaurants (including 14 hotels and 11 freestanding restaurants) in Beijing, China. The relationships among customer participation, interpersonal trust and employee innovative behavior were examined using structural models analyzed in AMOS 20.0.FindingsThe structural equation modeling results indicate that customers’ information and emotional participation in services significantly influence the innovative behavior of employees, whereas behavioral participation does not. In addition, a high level of interpersonal trust between customers and employees may increase employee innovative behaviors. Moreover, unlike cognitive trust, affective trust mediates the relationship between customer information or emotional participation and employee innovative behavior.Practical implicationsFindings indicate that service firms can encourage customers to participate actively in service co-creation; their participation in terms of information is encouraged to foster employee innovative behaviors by training employees and establishing an appropriate climate for information exchange. Moreover, service firms must pay attention to the emotions of customers during the service processes. Furthermore, the affective trust between customers and employees is significant to service firms, which need to take measures for employees to manage their relationships with customers well.Originality/valueBased on the concepts of service marketing and organizational behavior, this study contributes to the research on customer–employee co-production and employee innovative behavior from an interdisciplinary perspective. The study reveals the influencing mechanism of customer participation on employee innovative behavior and contributes to the research on customer–employee interpersonal trust. Previous studies emphasized the importance of trust among work group members in innovation, while this study supports the association between customer–employee interpersonal trust and employee innovative behaviors.
A multilevel investigation of China’s regional economic conditions on co-creation of dining experience and outcomes
Authors: Ji Mingjie , Wong IpKin Anthony , Eves Anita , Leong Aliana Man Wai 
Published: February 2018

Purpose
The purpose of this study is to investigate how the presence of other customers in restaurant social settings becomes a resource (referred to as “customer-to-customer interaction” or “C2CI”) to co-create an escape dining experience and stimulate dining outcomes, namely, food attachment and dining frequency. The relationships are further tested under the effects of regional economic conditions.

Design/methodology/approach
Data were collected by using a multi-step approach. The first data set was obtained through a personally administered survey, which included a sample of 356 Chinese tourists who dined at fine Western (i.e. Portuguese) restaurants in Macau. The second data set concerned economic statistics and was obtained from the statistics departments of mainland China and Taiwan. A multilevel design with hierarchical linear modeling was used to test the proposed model. Multilevel mediating and moderating effects were also examined.

Findings
The results suggest that customer escape dining experience significantly mediated the relationship between C2CI and food attachment, while food attachment fully mediated the relationship between customer escape experience and dining frequency. The multilevel effect of regional economic conditions played a significant role in moderating the C2CI–escape experience relationship in which the effect of C2CI was more salient for tourists from less economically developed regions in China. The experience–food attachment relationship was also contingent on the regional economic conditions in which the relationship was stronger for tourists from less economically developed areas. A multilevel mediating effect was also presented in the study.

Originality/value
The study contributes to the literature on experience co-creation in restaurant dining by exploring and testing the possibility of the presence of other customers to become a resource of experience co-creation, which is currently overlooked in the restaurant dining literature. The study advances the concept of co-creation by including the presence of other customers and restates the active role of diners in creating experiences. It also considers the existence of structural patterns in individualized experiences.
Value co-creation on social media
Authors: Lin Shan , Yang Shuai , Ma Minghui , Huang Jian 
Published: February 2018

Purpose
In recent years, hotels in China have been interested in leveraging social media platforms to facilitate interactions with and among consumers. Such brand engagement efforts on social media networks are believed to promote brands through co-creation of consumer experiences and values. This study was conducted in the context of Chinese hotels. The paper aims to identify two forms of brand engagement via social media platforms – consumer-initiated engagement and firm-initiated engagement – and to examine their effects on hotels’ display advertising effectiveness.

Design/methodology/approach
This study collected a comprehensive data set. First, the authors collected display advertisement data from two hotel chains in China. Second, the authors gathered the two hotels’ engagement data from Weibo. A generalized linear mixed model was used in data analysis.

Findings
The findings of the study indicate that both forms of brand engagement on social media network sites positively influence display advertising effectiveness. Moreover, for a strong brand, consumer-initiated engagement is more influential in increasing display advertising effectiveness; however, for a weak brand, firm-initiated engagement gains more clicks and conversions from advertisements.

Practical implications
As hotels in China continue to leverage online media platforms to reach, engage with and co-create value with potential and existing consumers, this study provides managers with insight as to how they can achieve higher advertising effectiveness by engaging with consumers on a consistent basis on social media.

Originality/value
This study mainly contributes to recent increasing research on engagement and value co-creation by providing a lens through which to assess the relationship between brand engagement via social media networks and online display advertising effectiveness.
When co-creation pays: stimulating engagement to increase revenues
Authors: Tu YaoJen , Neuhofer Barbara , Viglia Giampaolo 
Published: February 2018

Purpose
Customer willingness to pay (WTP) was initially set out to estimate the perceived value from a purchasing experience. However, purchasing decisions have changed as value co-creation has been increasingly applied in the hospitality industry. In adopting a service-dominant (S-D) logic lens, this paper aims to empirically test how co-creation affects WTP through customer engagement (CE).

Design/methodology/approach
The context for the empirical analysis is the Chinese market, one of the largest online purchasing markets that has been significantly transformed since the proliferation of co-creation. The study is a within-design online experiment with 488 Chinese participants. The analysis makes use of mediation models to evaluate the proposed mechanisms behind co-creation, CE and the moderated role of frequency of stay, and their impacts on WTP.

Findings
The data confirm the hypothesised positive impact of value co-creation on customer WTP. This impact is fully mediated by CE, i.e. CE is the mechanism behind a higher WTP propensity for co-created hotel rooms. Notably, the frequency of stay at a hotel, thus positively influencing WTP, does not have a moderated mediation effect on this relationship.

Originality/value
Limited research to date has investigated the price effectiveness of value co-creation in the hospitality context. This study contributes to the S-D logic and value co-creation discourses by testing the effectiveness of these concepts in relation to customer pricing decisions. This study empirically confirms the hypothesised model and provides recommendations for hospitality research and practice.
A taxonomy of value co-creation on Weibo – a communication perspective
Authors: Ge Jing , Gretzel Ulrike 
Published: February 2018

Purpose
This paper aims to develop a taxonomy of value co-creation types occurring in firm-customer interactions on social media.

Design/methodology/approach
In total, 570 destination marketing organization (DMO)-initiated posts on Weibo and 3,137 responses were collected to develop a taxonomy by conducting qualitative empirical-to-conceptual analysis. To apply the taxonomy through conceptual-to-empirical analysis, 100 DMO-initiated posts and 823 responses were collected.

Findings
The communication-focused value co-creation taxonomy shows a variety of co-creators, verbal and non-verbal communicative co-creation actions facilitated by social media, and different co-created value types.

Research limitations/implications
This study used a single social media platform and selected three DMOs’ Weibo accounts. Future research should focus on other types of firms and different social media platforms.

Practical implications
This study used a single social media platform and selected three DMOs’ Weibo accounts. Future research should focus on other types of firms and different social media platforms.

Originality/value
This study enriches the tourism literature and the general marketing literature by examining value co-creation from a communication perspective and provides a comprehensive classification of value co-creation opportunities on social media.
Risk factors influencing environmental protest severity in China
Authors: Xue Ming , Shen Huizhang , Zhao Jidi 
Published: February 2018

Purpose
Using protest event analysis, this study aims to investigate which risk factors influence the severity of environmental protests in China, thus filling a gap in China-related environmental protest literature.

Design/methodology/approach
Using a database derived from media coverage of 129 environmental protests in China from 2009 to 2015, this empirical study identifies underlying risk factors influencing environmental protest severity, quantifies these protest cases and verifies the associations between risk factors and severity using ordered logistic regression.

Findings
The results show that higher environmental health threat, economic loss, distrust of local government, lack of local governmental response, improper local government action and higher population density are likely to increase environmental protest severity; however, contrary to expectations, environmental information disclosure has no significant effect. These findings illuminate the vital and variational role of local government throughout all stages of the evolutionary process in environmental protests. Moreover, public distrust of local government is the principal cause of these protests.

Originality/value
This study enhances the understanding of how Chinese environmental protests arise from the identified risk factors and contributes to quantitative multi-case research in this area. Furthermore, the findings may help local governments in China, as well as in other countries, to enact positive measures to prevent serious environmental protests and improve their ability to address the environmental problems that cause protests. More effective governance can decrease the number and severity of environmental protests and thus promote social stability.
The applicability of growth machine theory to the knowledge economy
Authors: Chilton Kenneth , Jung Kyujin 
Published: February 2018

Purpose
Neoliberal urban regimes focus on redeveloping downtowns to compete for economic development. Chattanooga has been lauded by urban development organizations such as Brookings and the Urban Institute for its public-private partnership model dubbed the Chattanooga Way. The paper aims to discuss these issues.

Design/methodology/approach
In this paper, the authors use social network analysis to analyze elites highly involved in local economic development, education policy and social entrepreneurship in Chattanooga, TN.

Findings
The results suggest a strong group of nonelected local elites dominate policymaking in policy arenas traditionally reserved for elected elites. The overlap between elites who shape local policy and elites who fiscally benefit from local policies raises troubling questions for local democracy, public accountability and transparency.

Originality/value
This analysis is valuable to public policy scholars who are interested in analyzing neoliberal coalitions and their impact on local development initiatives.