bluuebunny/crossref_metadata_2025_split huggingface dataset:
CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection
Authors: Vaneckova Tereza , Bezdekova Jaroslava , Tvrdonova Michaela , Vlcnovska Marcela , Novotna Veronika , Neuman Jan , Stossova Aneta , Kanicky Viktor , Adam Vojtech , Vaculovicova Marketa , Vaculovic Tomas 
Published: August 2019

AbstractFor the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-antibody and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10–250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.
Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level
Authors: Domenici F. , Capocefalo A. , Brasili F. , Bedini A. , Giliberti C. , Palomba R. , Silvestri I. , Scarpa S. , Morrone S. , Paradossi G. , Frogley M. D. , Cinque G. 
Published: August 2019

AbstractUltrasound (US) induced transient membrane permeabilisation has emerged as a hugely promising tool for the delivery of exogenous vectors through the cytoplasmic membrane, paving the way to the design of novel anticancer strategies by targeting functional nanomaterials to specific biological sites. An essential step towards this end is the detailed recognition of suitably marked nanoparticles in sonoporated cells and the investigation of the potential related biological effects. By taking advantage of Synchrotron Radiation Fourier Transform Infrared micro-spectroscopy (SR-microFTIR) in providing highly sensitive analysis at the single cell level, we studied the internalisation of a nanoprobe within fibroblasts (NIH-3T3) promoted by low-intensity US. To this aim we employed 20 nm gold nanoparticles conjugated with the IR marker 4-aminothiophenol. The significant Surface Enhanced Infrared Absorption provided by the nanoprobes, with an absorbance increase up to two orders of magnitude, allowed us to efficiently recognise their inclusion within cells. Notably, the selective and stable SR-microFTIR detection from single cells that have internalised the nanoprobe exhibited clear changes in both shape and intensity of the spectral profile, highlighting the occurrence of biological effects. Flow cytometry, immunofluorescence and murine cytokinesis-block micronucleus assays confirmed the presence of slight but significant cytotoxic and genotoxic events associated with the US-nanoprobe combined treatments. Our results can provide novel hints towards US and nanomedicine combined strategies for cell spectral imaging as well as drug delivery-based therapies.
Rumen bacterial community responses to DPA, EPA and DHA in cattle and sheep: A comparative in vitro study
Authors: Carreño D. , Toral P. G. , Pinloche E. , Belenguer A. , Yáñez-Ruiz D. R. , Hervás G. , McEwan N. R. , Newbold C. J. , Frutos P. 
Published: August 2019

AbstractThe role of marine lipids as modulators of ruminal biohydrogenation of dietary unsaturated fatty acids may be explained by the effects of their n-3 polyunsaturated fatty acids (PUFA) on the bacterial community. However, the impact of individual PUFA has barely been examined, and it is uncertain which bacteria are truly involved in biohydrogenation. In addition, despite interspecies differences in rumen bacterial composition, we are not aware of any direct comparison of bovine and ovine responses to dietary PUFA. Therefore, rumen fluid from cannulated cattle and sheep were used as inocula to examine in vitro the effect of 20:5n-3 (EPA), 22:5n-3 (DPA), and 22:6n-3 (DHA) on the bacterial community. Amplicon 16 S rRNA sequencing suggested that EPA and DHA had a greater contribution to the action of marine lipids than DPA both in cattle and sheep. Certain effects were exclusive to each ruminant species, which underlines the complexity of rumen microbial responses to dietary fatty acids. Based on changes in bacterial abundance, Barnesiella, Prevotella, Paraprevotella, Hallela, Anaerovorax, Succiniclasticum, Ruminococcus and Ruminobacter may be involved in the ruminal response in biohydrogenation to the addition of marine lipids, but further research is necessary to confirm their actual role in ruminal lipid metabolism.
Functional genomic characterization of metallothioneins in brown trout (Salmo trutta L.). using synthetic genetic analysis
Authors: Paris Josephine R. , Usher Jane 
Published: August 2019

AbstractMetal pollution has made a significant impact on the earth’s ecosystems and tolerance to metals in a wide variety of species has evolved. Metallothioneins, a group of cysteine-rich metal-ion binding proteins, are known to be a key physiological mechanism in regulating protection against metal toxicity. Many rivers across the southwest of England are detrimentally affected by metal pollution, but brown trout (Salmo trutta L.) populations are known to reside within them. In this body of work, two isoforms of metallothionein (MetA and MetB) isolated from trout occupying a polluted and a control river are examined. Using synthetic genetic array (SGA) analyses in the model yeast, Saccharomyces cerevisiae, functional genomics is used to explore the role of metallothionein isoforms in driving metal tolerance. By harnessing this experimental system, S. cerevisiae is used to (i) determine the genetic interaction maps of MetA and MetB isoforms; (ii) identify differences between the genetic interactions in both isoforms and (iii) demonstrate that pre-exposure to metals in metal-tolerant trout influences these interactions. By using a functional genomics approach leveraged from the model yeast Saccharomyces cerevisiae, we demonstrate how such approaches could be used in understanding the ecology and evolution of a non-model species.
Climate change-based models predict range shifts in the distribution of the only Asian plethodontid salamander: Karsenia koreana
Authors: Borzée Amaël , Andersen Desiree , Groffen Jordy , Kim Hyun-Tae , Bae Yoonhyuk , Jang Yikweon 
Published: August 2019

AbstractPopulations see their range fluctuate in relation to environmental variations, including climate change, and their survival is linked to the maintenance of large enough populations and broad enough distributions during these variations. Most amphibian populations are threatened by numerous ecological and anthropogenic variables acting in synergy with climate change. Accumulating basic ecological data such as range enables the development of population and range dynamics, themselves resulting on adequate conservation plans.Karsenia koreanais the only known Asian plethodontic salamander, occurring in a very restricted area only. Based on presence data, we created an ecological model using six bioclimatic factors with low multicollinearity to define the adequate habitat of the species, and we modelled the predicted suitability of the Korean landscape following four Representative Concentration Pathways (RCPs) predicting climate change scenarios based on CO2concentrations in 2050 and 2070. The maximum entropy model for the current distribution produced a landscape suitability considerably wider than the current known distribution. The projected ranges for each RCP indicated marked increases, decreases and shifts in areas with suitable landscapes due to climate change. The lowest RCP prediction resulted in an increase in suitable area, although potentially without connectivity with current populations, while the highest RCP predictions resulted in a decrease. Our results highlight the potential negative impact of climate change, thus requiring updates in conservation plans forK. koreana. The methods used here can be replicated with any land-dwelling species, and our results reflect expected range shifts for most amphibians of the northern hemisphere.
Dickkopf-related protein 1 is a progestomedin acting on the bovine embryo during the morula-to-blastocyst transition to program trophoblast elongation
Authors: Tríbulo Paula , Rabaglino María Belen , Bo Martin Bonet , Carvalheira Luciano de R. , Bishop Jeanette V. , Hansen Thomas R. , Hansen Peter J. 
Published: August 2019

AbstractProgesterone regulates the endometrium to support pregnancy establishment and maintenance. In the ruminant, one action of progesterone early in pregnancy is to alter embryonic development and hasten the process of trophoblast elongation around day 14–15 of pregnancy, which is required for maternal recognition of pregnancy. Here we demonstrate that the WNT antagonist DKK1, whose expression is increased by progesterone treatment, can act on the bovine embryo during day 5 to 7.5 of development (the morula to blastocyst stage) to promote embryonic elongation on day 15 of pregnancy. Embryos were produced in vitro and exposed to 0 or 100 ng/ml recombinant human DKK1 from day 5 to 7.5 of culture. Blastocysts were transferred into synchronized recipient cows on day 7.5 (n = 23 for control and 17 for DKK1). On day 15, cows were slaughtered and embryos recovered by flushing the uterus. Embryo recovery was n = 11 for controls (48% recovery) and n = 11 for DKK1 (65% recovery). Except for two DKK1 embryos, all embryos were filamentous. Treatment with DKK1 increased (P = 0.007) the length of filamentous embryos from 43.9 mm to 117.4 mm and the intrauterine content of the maternal recognition of pregnancy signal IFNT (P = 0.01) from 4.9 µg to 16.6 µg. Determination of differentially expressed genes (DEG), using the R environment, revealed 473 DEG at p < 0.05 but none at FDR < 0.05, suggesting that DKK1 did not strongly modify the embryo transcriptome at the time it was measured. However, samples clustered apart in a multidimensional scaling analyisis. Weighted gene co-expression analysis of the transcriptome of filamentous embryos revealed a subset of genes that were related to embryo length, with identification of a significant module of genes in the DKK1 group only. Thus, several of the differences between DKK1 and control groups in gene expression were due to differences in embryo length. In conclusion, DKK1 can act on the morula-to-blastocyst stage embryo to modify subsequent trophoblast elongation. Higher pregnancy rates associated with transfer of DKK1-treated embryos may be due in part to enhancements of trophoblast growth and antiluteolytic signaling through IFNT secretion. Given that progesterone can regulate both timing of trophoblast elongation and DKK1 expression, DKK1 may be a mediator of progesterone effects on embryonic development.
Inner retinal thinning as a biomarker for cognitive impairment in de novo Parkinson’s disease
Authors: Sung Mi Sun , Choi Seong-Min , Kim Jonghwa , Ha Jun Young , Kim Byeong-Chae , Heo Hwan , Park Sang Woo 
Published: August 2019

AbstractWe investigated the association between retinal changes measured using optical coherence tomography (OCT) and diverse clinical grading scales in patients with Parkinson’s disease (PD). Seventy-four eyes of 74 patients withde novoPD and 53 eyes of age-matched control subjects were included. The thickness of the peripapillary retinal nerve fiber layer (pRNFL) and macular ganglion cell-inner plexiform layer (mGCIPL) were measured. We analyzed the correlations between the clinical PD grading scales and OCT parameters, and between the OCT parameters and volumetric data in the cerebral cortical and subcortical structures. The area under the receiver operating characteristic curve (AUC) was calculated for diagnosing cognitive impairment in patients with PD. Statistically significant reductions in the thickness of average, temporal, and inferior pRNFL and overall mGCIPL were observed in patients with PD. The Montreal Cognitive Assessment score was significantly associated with mGCIPL thinning. The AUC of the mGCIPL parameters for diagnosing cognitive impairment in patients with PD ranged from 0.651 to 0.760. Moreover, thinning of the mGCIPL was significantly associated with the volumetric parameters of associated brain structures. Our findings highlight the clinical implications of OCT measurements as a potential biomarker for early detection of cognitive impairment in patients with PD.
Variable ability of rapid tests to detect Mycobacterium tuberculosis rpoB mutations conferring phenotypically occult rifampicin resistance
Authors: Torrea Gabriela , Ng Kamela C. S. , Van Deun Armand , André Emmanuel , Kaisergruber Justine , Ssengooba Willy , Desmaretz Christel , Gabriels Siemon , Driesen Michèle , Diels Maren , Asnong Sylvie , Fissette Kristina , Gumusboga Mourad , Rigouts Leen , Affolabi Dissou , Joloba Moses , De Jong Bouke C. 
Published: August 2019

AbstractWe compared the ability of commercial and non-commercial, phenotypic and genotypic rapid drug susceptibility tests (DSTs) to detect rifampicin resistance (RR)-conferring ‘disputed’ mutations frequently missed by Mycobacterium Growth Indicator Tube (MGIT), namely L430P, D435Y, L452P, and I491F. Strains with mutation S450L served as positive control while wild-types were used as negative control. Of the 38 mutant strains, 5.7% were classified as RR by MGIT, 16.2% by Trek Sensititre MYCOTB MIC plate, 19.4% by resazurin microtiter plate assay (REMA), 50.0% by nitrate reductase assay (NRA), and 62.2% by microscopic observation direct susceptibility testing (MODS). Reducing MGIT rifampicin concentration to 0.5 µg/ml, and/or increasing incubation time, enhanced detection of disputed mutations from 5.7% to at least 65.7%, particularly for mutation I491F (from 0.0 to 75.0%). Compared with MGIT at standard pre-set time with 0.25 µg/ml ECOFF as breakpoint, we found a statistically significant increase in the ability of MGIT to resolve disputed mutants and WT strains at extended incubation period of 15 and 21 days, with 0.5 µg/ml and 1 µg/ml ECOFF respectively. MODS detected 75.0% of the I491F strains and NRA 62.5%, while it was predictably missed by all molecular assays. Xpert MTB/RIF, Xpert Ultra, and GenoscholarTB-NTM + MDRTB detected all mutations within the 81 bp RR determining region. Only GenoType MTBDRplus version 2 missed mutation L430P in 2 of 11 strains. Phenotypic and genotypic DSTs varied greatly in detecting occult rifampicin resistance. None of these methods detected all disputed mutations without misclassifying wild-type strains.
The influence of the chosen in vitro bone simulation model on intraosseous temperatures and drilling times
Authors: Szalma József , Lovász Bálint V. , Vajta László , Soós Balázs , Lempel Edina , Möhlhenrich Stephan C. 
Published: August 2019

AbstractThere is no consensus about the most suitable in vitro simulating material investigating heat generation during bone preparation. The aim was to compare heat increases and drilling times of bone removals in different bone simulating materials and to compare them to fresh human cadaver bone. A cavity was drilled in the following samples: (1) bovine rib; (2) pig rib; (3) 20 PCF (lb/ft3) polyurethane (PU) block with 3 mm (50 PCF) cortical layer; (4) 20PCF PU without cortical; (5) 30 PCF PU with 2 mm (40 PCF) cortical; (6) 30 PCF PU with 1 mm (40 PCF) cortical; (7) 30PCF PU without cortical; (8) poly-methyl-methacrylate (PMMA); (9) fresh human cadaver rib. Data were analyzed with ANOVA followed by Tukey’s post hoc tests. P < 0.05 was considered significant. Highest heat increases and slowest drilling times were found in bovine ribs (p < 0.001). Regarding temperatures, human ribs were comparable to the pig rib and to PUs having cortical layers. Considering drilling times, the human rib was only comparable to the 20 PCF PU with 3 mm cortical and to 30 PCF PU without cortical. By the tested in vitro bone removals, only the 20 PCF PU with 3 mm cortical was able to simulate human ribs, considering both temperature increases and drilling times.
High-efficiency genomic editing in Epstein-Barr virus-transformed lymphoblastoid B cells using a single-stranded donor oligonucleotide strategy
Authors: Johnston Andrew D. , Simões-Pires Claudia A. , Suzuki Masako , Greally John M. 
Published: August 2019

AbstractWhile human lymphoblastoid cell lines represent a valuable resource for population genetic studies, they have usually been regarded as difficult for CRISPR-mediated genomic editing because of very inefficient DNA transfection and retroviral or lentiviral transduction in these cells, which becomes a substantial problem when multiple constructs need to be co-expressed. Here we describe a protocol using a single-stranded donor oligonucleotide strategy for ‘scarless’ editing in lymphoblastoid cells, yielding 12/60 (20%) of clones with homology-directed recombination, when rates of <5–10% are frequently typical for many other cell types. The protocol does not require the use of lentiviruses or stable transfection, permitting lymphoblastoid cell lines to be used for CRISPR-mediated genomic targeting and screening in population genetic studies.
Modified-Bolus Placement as a Therapeutic Tool in the Treatment of Pediatric Feeding Disorders: Analysis From a Retrospective Chart Review
Authors: Volkert Valerie M. , Sharp William G. , Clark Michele Cole , Spinks Hailey , Rubio Emily Kate , McCracken Courtney , Bryan Leah 
Published: August 2019

Purpose
Recognizing the need to advance the treatment of pediatric feeding disorders, an emerging area of inquiry focuses on therapeutic techniques that address expulsion and packing possibly associated with oral–motor dysfunction. In the current study, we documented the use of modified-bolus placement in the treatment of pediatric feeding disorders at an intensive, multidisciplinary day-treatment center over a 26-month period.

Method
The retrospective observational cohort study involved patients admitted for the assessment and treatment of chronic food refusal from August 2013 to October 2015.

Results

Patients (
n
= 23) who received modified bolus placement displayed moderate-to-severe oral–motor deficits. Use of modified-bolus placement was associated with reduced expulsion and packing of bites during meals, and treatment gains coincided with a threefold increase in grams consumed per meal.

Conclusion
Results provide additional support for the potential therapeutic benefits of enhancing bolus placement onto the tongue as a means to improve mealtime performance in children with pediatric feeding disorders when included as an adjunct to more traditional behavioral approaches.
State estimation of 500 kV sulphur hexafluoride high‐voltage CBs based on Bayesian probability and neural network
Authors: Geng Sujie , Wang Xiuli , Sun Peng 
Published: August 2019

Circuit breakers (CBs) are of vital importance for the stability of power systems, and a new two‐stage hierarchical state estimation method is proposed for 500 kV sulphur hexafluoride CBs based on Bayesian probability and perceptron neural networks. On the basis of the samples collected from Yunnan Power Grid in China, a new indicator system is constructed by association rules. Bayesian probability is applied to measure the correlation between the individual indicators and comprehensive indicators at the same status level, to weigh the individual indicators. Also, an adaptive perceptron is improved to train the weights of comprehensive indicators in different operational conditions, to eliminate the influence of the imbalance problem of relative deterioration. Then, the operating state of equipment can be inferred according to the calculated comprehensive scores. Finally, taking the actual operating equipment as an example, the effectiveness of this proposed method is proved by sample tests and comparison with other existing linear methods.
Circulating CTRP6 Levels are Increased in Overweight or Obese Chinese Individuals and Associated with Insulin Resistance Parameters: A Pilot Study
Authors: Liao Xin , Liu Sha , Tang Xuejiao , Yang Dan , Liu Hua , Gao Lin , Yang Gangyi 
Published: August 2019

Abstract
Aims CTRP6, a newly discovered adipokine, has been found to be a regulator for energy homeostasis. However, the association between circulating CTRP6 and obesity in humans is still unclear.
Methods 256 individuals, including 185 overweight/obese (OW/OB) and 71 normal weight adults, were recruited for this study. Circulating concentrations of CTRP6 and adiponectin (Adipoq) were examined by ELISA.
Results Serum CTRP6 levels in obese individuals were significantly increased compared with those in healthy individuals (506.1±134.9 vs.363.3±80.5 ng/mL, P<0.01). Conversely, serum Adipoq concentrations in OW/OB individuals were markedly decreased compared with healthy controls [20.8 (12.1–29.3) vs. 14.1
(
8.61–17.7)
;

P<0.01]. Correlation analysis revealed that there was a positive relationship between circulating CTRP6 and age, BMI, Fat%, LDL-C, TG, WHR, TC, FBG, FIns, HOMA-IR and HbA1c, but there was an inverse correlation with Adipoq and HDL-C. Logistic regression analysis revealed that high serum CTRP6 levels are markedly associated with OW/OB. Finally, ROC curve analysis showed that the cut-off value for serum CTRP6 for prediction of IR is 518 ng/mL.
Conclusions CTRP6 may be a marker related to OW/OB.
Der Lipödemschmerz, seine Folgen auf die Lebensqualität
betroffener Patientinnen – Ergebnisse einer Patientenbefragung mittels
Schmerzfragebogen
Authors: Gensior Matthias H.L. , Cornely Manuel 
Published: August 2019

ZusammenfassungBei 640 Patientinnen einer Fachklinik für operative Lymphologie erfolgte mittels
Fragebogen der Deutschen Schmerzgesellschaft e. V. eine Befragung. Neben Fragen
zum Schmerz und zur Schmerzcharakteristik wurden gleichzeitig noch
demographische Daten miterhoben. Es ergab sich, dass nur bei etwas über 50 %
eine echte Adipositas nachgewiesen werden konnte.Lipödem und Adipositas müssen
als unabhängige Krankheitsbilder gewertet werden. Der Schmerz wurde überwiegend
als drückend und ziehend empfunden. Attribute wie klopfend oder pochend, passend
zu einer akuten Entzündung, erfuhr die Wertung „nichtzutreffend“. Die
Beschwerdesymptomatik war unabhängig vom BMI, der bei der Lipohyperplasie
dolorosa nur bedingt verwertbar ist. Insgesamt ist das Leitsymptom „Schmerz“
sehr facettenreich, das angeborene, nicht erworbene Lipödemfett der Extremitäten
führt zu einer deutlichen Beeinträchtigung der Aktivitäten allgemein, als auch
im Freizeitbereich. Die durch den GBA initiierte Studie muss daher kritisch
gesehen werden. Da bislang keine objektivierbaren Befunde beim Lipödem erhoben
werden können, ist eine subtile Befragung betroffener Patientinnen zur
Diagnosestellung notwendig.