bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early ovarian differentiation in the tammar wallaby and the effects of exposure to bisphenol A
Authors: Peiris Mario T , Chen Yu , Shaw Geoff , Renfree Marilyn B 
Published: November 2024

In briefOvarian differentiation in tammar wallabies begins after birth, allowing direct experimental manipulation during the period of differentiation. Using this unique model, we demonstrate that the environmental endocrine-disrupting chemical bisphenol A (BPA) interferes with normal morphological development and gene expression in the developing ovary.AbstractBPA, an environmental endocrine disruptor, is used widely in the manufacturing of various plastics. While BPA can have detrimental effects on fertility and reproductive health, the effects of BPA on early ovarian differentiation in mammals remain unclear. Marsupials have undifferentiated gonads at birth, so this study investigated the gross morphology, protein localisation of FOXL2 and FST and the expression profile of key ovarian differentiating genes FOXL2, WNT4, FST, ESR1 and ESR2 every 2 days from the day of birth to day 10 postpartum (pp) in the marsupial tammar wallaby. A second group of newborn female pouch young were treated with 50 μg/kg of BPA daily from day 0 to day 10 pp, and the morphology and gene expression were examined at day 10 pp. Ovigerous cords in tammar ovaries were first formed between day 2 and day 4 pp. FOXL2 localisation became nuclear by day 4 in pre-granulosa cells. FST was initially in the cytoplasm of pre-granulosa cells at day 2 pp but was then secreted into the extracellular matrix in ovaries by day 10 pp. FOXL2, FST, ESR1 and ESR2 mRNA were upregulated in ovaries around day 2–4 pp, indicating that ovarian differentiation in the tammar begins from day 2 to day 4 pp. Interestingly, BPA treatment from day 0 to day 10 pp blocked the morphological differentiation of the cortex and medulla and ovigerous cord formation and downregulated the expression of FST and FOXL2 at day 10 pp.
Luddism for Community Informatics
Authors: Stoecker Randy 
Published: November 2024

This paper begins with a reflection on my own history in community informatics, including my recent turn to an appreciation of Luddism. It then discusses a concern that community informatics may be contributing to an expansion of Marx’s four forms of alienation. The paper then moves into a discussion of the data-information-knowledge-wisdom “pyramid” as a potential framework for a revised approach to community informatics and contrasts that revised approach with the potentially unsustainable approach of the field currently.
Successful Implementation of Nirsevimab and Factors Influencing Uptake in Neonatal Care
Authors: Puckett Lauren , Kushner Lauren E. , Bio Laura , Cornell Sean , Wood Matthew , Schwenk Hayden T. 
Published: November 2024

OBJECTIVE
To describe the implementation of nirsevimab for the prevention of respiratory syncytial virus-associated lower respiratory tract disease in a pediatric hospital, focusing on strategies to ensure equitable access and address logistic challenges. Additionally, we aimed to identify predictors of nirsevimab deferral among eligible infants.

METHODS
Our hospital implemented a universal immunization campaign to all eligible infants, including those discharged from the newborn nursery, intermediate care nursery, and neonatal intensive care unit. We identified key drivers and barriers, formed a multidisciplinary team, and applied a systematic approach for integration of nirsevimab orders into existing workflows. We developed and disseminated educational resources for staff and caregivers. After the implementation, we conducted univariable and multivariable analyses to identify predictors of nirsevimab deferral to evaluate implementation success and possible gaps.

RESULTS
Despite challenges, we offered nirsevimab to 99% of eligible infants prior to discharge from the newborn nursery, intermediate care nursery, and neonatal intensive care unit with 71% receiving the immunization. On the multivariable analysis, independent predictors of nirsevimab deferral included preferred language of English, deferral of hepatitis B vaccine, discharge from the newborn nursery, and public insurance.

CONCLUSIONS
Our implementation strategy ensured equitable access to nirsevimab for newborns with both our high uptake and acceptance rate underscoring the effectiveness of our approach. Key strategies for success included early stakeholder engagement, multidisciplinary collaboration, and proactive logistic planning. Our approach serves as a model for other institutions to offer nirsevimab prior to hospital discharge and highlights the importance of addressing both clinical and socioeconomic barriers.
Skam
Authors: Mathiasen Henriette Pagh 
Published: November 2024

En nyere undersøgelse fra Institut for Menneskerettigheder viser, at etniske minoriteter oplever diskrimination og forskelsbehandling i Danmark.
Med udgangspunkt i personlige tanker og følelser, som opstod i kølvandet på en vejledning over telefon med en minoritetskvinde, reflekterer essayet over oplevelsen af skam og forforståelser i vejledningsrummet. Teksten beskriver hvordan irritation og modstand i samtalen affødte en oplevelse af skam, som skabte tvivl om egen professionelle dømmekraft og vejledningssamtalens kvalitet hos forfatteren.
Essayet dykker ned i udfordringerne i interkulturel vejledning på telefon, og reflekterer over hvordan forforståelser og sprogbarrierer kan medføre mentaliseringssvigt hos vejlederen. Den særlige dynamik i en telefonisk vejledning udforskes, og teksten undersøger fordele og ulemper ved denne type vejledning.
Teksten trækker på teorier om skam og etik med teoretisk afsæt i bl.a. Hans-Georg Gadamer og K.E. Løgstrup. Forfatteren reflekterer over hvordan ubevidste biases påvirker relationen mellem vejleder og den vejledningssøgende, og argumenterer for at vejlederens skam kan medføre professionel udvikling. Gennem refleksioner om ansvar og strukturel ulighed undersøger forfatteren, hvordan skam kan fungere som et moralsk kompas, der tvinger os til at arbejde frem mod en mere socialt retfærdig vejledningspraksis.
INTERFACE DA INTELIGÊNCIA ARTIFICIAL E GENÔMICA FUNCIONAL: ÊNFASE NO SISTEMA CRISPR
Authors: Matias Amanda Gilvani Cordeiro , Carvalho Danila Guimarães , Silveira Silvana Braga da 
Published: November 2024

A genômica é um campo interdisciplinar da biologia molecular que estuda a estrutura, função, evolução, mapeamento e edição de genomas dos organismos, pela decodificação dos genomas dos seres biológicos para análises de dados. Sua interface com a inteligência artificial tem sido intensificada a partir de estratégias de Deep Learning (DL) na abordagem do Big Data e no sistema Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) desponta possibilidades revolucionárias na biotecnologia e medicina. O objetivo foi descrever o perfil do uso da inteligência artificial aplicada a genômica funcional e no sistema de edição genética CRISPR. Trata-se de uma revisão de escopo que selecionou artigos de 2020-2024 com buscas nas bases da SciELO, NCBI/PubMed®, Science Direct. A combinação mnemônica PCC (População, Contexto, Conceito) foi utilizada para definir a questão norteadora da pesquisa. A revisão foi descrita conforme diretrizes do checklist Preferred Reporting Items for Systematic Reviews and Meta-analyses for Scoping Reviews (PRISMA-ScR). Foram inclusos 20 artigos que atenderam aos critérios da pesquisa e após analisar o conteúdo sobre a conexão da inteligência artificial (IA) ​​e as ciências ômicas, verificou-se notórios avanços referente ao aumento da precisão e alcance das tecnologias assistidas pelo aprendizado de máquina. Conclui-se que algoritmos treinados permitem o aprendizado de máquina na mineração de grande volume de dados e proporciona análises preditivas e mais precisas e supera métodos tradicionais. A IA amplia a capacidade de dispositivos tecnológicos nas ciências ômicas e o rendimento; no sistema CRISPR supera métodos tradicionais em precisão, padrões generalizáveis e compreensão do design de RNA-guia.
Planowane rozszerzenie „Unia 30+” a alokacja miejsc w Parlamencie Europejskim
Authors: Barcz Jan 
Published: November 2024

Przedmiot artykułu dotyczy formuły alokacji miejsc w Parlamencie Europejskim w świetle planowanego rozszerzenia Unii Europejskiej. Przedstawiono przebieg prac nad tym zagadnieniem w świetle postanowień Traktatu z Nicei i Traktatu z Lizbony. Wskazano na znaczenie tego problemu, zwłaszcza wobec nowej formuły podejmowania decyzji w Radzie UE większością kwalifikowaną. Niemniej uzgodnienie stałego systemu gwarantującego przestrzeganie zasady degresywnej proporcjonalności przy alokacji miejsc w Parlamencie Europejskim jest bardzo trudne. Należy raczej zakładać, że podział miejsc w Parlamencie Europejskim podczas finalizacji planowanego rozszerzenia Unii będzie, jak dotychczas, przede wszystkim wynikiem kontraktu politycznego między państwami członkowskimi.
Bills to Restrict Access to and Harm From Indoor Tanning Facilities in US State Legislatures, 1992‒2023
Authors: Buller David B. , Berteletti Julia , Heckman Carolyn , Schroth Kevin R. J. , Geller Alan C. , Stapleton Jerod L. , Adjei Irene , Mitarotondo Anna , Guild Samantha R. , Gershenwald Jeffrey E. , Dellavalle Robert , Pagoto Sherry 
Published: November 2024

Objectives. To describe progression, content, and stringency of state legislation regulating indoor tanning and association with state government political party leadership. Methods. Trained research assistants used legal mapping methods to code legislative bills on indoor tanning introduced in US states, the District of Columbia, and Puerto Rico. We calculated composite scores on the stringency of age restrictions and of warnings, operator requirements, and enforcement. We evaluated associations of the political party of the legislative sponsor and legislature majority. Results. Between 1992 and 2023, 184 bills were introduced in 49 of 50 states and DC (56 laws were enacted, and 126 bills failed). An under-18 ban was enacted in 22 states and DC. Party affiliation of the bill sponsor and legislature majority combined to affect bill passage and age restrictions. Conclusions. In many states, it took several years and proposed bills before a law on indoor tanning was enacted. Enacted bills were more stringent than failed bills. Public Health Implications. Increasing support for stringent regulations on indoor tanning is evident and may motivate other states or the federal government to prohibit minors from using indoor tanning facilities. ( Am J Public Health. 2025;115(2):191–200. https://doi.org/10.2105/AJPH.2024.307894 )
Wellbore Transmissibility from Afterflow-Dominated Pressure Buildup Data
Authors: McKinley R. M. 
Published: November 2024

Abstract
This paper shows how pressure buildup data distorted by afterflow can be analyzed directly to give a numerical value for effective wellbore transmissibility. Because such data are directly indicative of wellbore-limited productivity, the method is helpful in assessing the need for and the effectiveness of a well stimulation. Alternatively, it is a method for interpreting that portion of a pressure buildup curve not subject to Horner analysis.
The method does not require any estimates of formation or wellbore properties for the analysis. The procedure consists of first plotting pressure buildup from flowing pressure as a function of shut-in time on a log-log scale and, second, matching the data points to one of a set of parametric type curves. From this match, a numerical value is calculated for wellbore transmissibility. Finally, the shape of the buildup curve indicates either wellbore damage or stimulation.
The paper describes the generation and use of type curves with computed data illustrating the effects of wellbore damage or stimulation. A variety of field examples demonstrate the practical utility of the method.
Determinants of Plasmodium infection prevalence using microscopy and nested polymerase chain reaction (nPCR) among febrile children suspected of malaria in Northwest Ethiopia
Authors: Getie Sisay , Getenet Gebeyaw , Worku Ligabaw , Birhanie Meseret , Meda Aline Lamien , Abere Aberham , Noedl Harald 
Published: November 2024

Abstract

Background
Malaria in children remains a major public health problem in the global, especially in sub-Saharan Africa. WHO reported an annually declining burden of malaria in Ethiopia. Despite these, different parts of the country reported a contradictory result, and malaria remains to be the main cause of morbidity among children. However, Microscopic examination of peripheral blood smear produces reliable results. Therefore, the aim of this study was to compare the performance of microscopy and nested polymerase chain reaction (nPCR) to detect malaria suspected febrile children.
Methods
A health institution-based cross-sectional study was conducted in northwest Ethiopia. A total of 302 malaria suspected participants were included. The capillary blood sample was collected from each study subject for the confirmation of malaria and a questionnaire was used to collect associated risk factors. Data analysis using SPSS version 23, Chi-square and multivariate analysis were performed for the comparison of categorical variables and assessment of associated risk factors, respectively. 𝑃 values less than 0.05 were taken as statistically significant. R
esults:
The overall prevalence of malaria was 22.2% and 18.2% by microscopy and nested polymerase chain reaction (nPCR), respectively. In nPCR malaria was found in 19.2% of males and 17.3% of female study subjects while the highest prevalence (25.6%) was observed among the age group ≥ 5 years old. Children in the age group between 1–5 years old were approximately 6 times more likely to be affected by malaria. And children who had no habited of bed net usages at all times were 12 times more likely to be contracted with malaria.
Conclusion
This study revealed that malaria infection among children seeks emphasis and need to specify the bottlenecks of all universal health coverage and community health awareness. In addition, the study suggested the need for adequate professional training towards clinical management of malaria to support unbiased diagnosis and treatment of childhood malaria.
Exploring Motivation and Barriers to Physical Activity among Adults Saudi Women
Authors: Alajlan Sarah , ALJOHANI obaidalah , Boughattas Wissal 
Published: November 2024

Abstract

This study aimed to discover Saudi women’s perceptions of their motivation to engage in physical activity and the challenges of engaging in physical activity at Taif University. The research also aimed to test whether there were significant differences among the variables due to age, type of work, and marital status, as well as to clarify whether the women were engaged in physical activity or sports. Moreover, the research investigated whether there was a statistically significant correlation between Saudi women’s perceptions of their motivation to engage in physical activity and the challenges of engaging in physical activity at Taif University. This study used Self-determination theory as the theoretical framework for understanding motivation. A self-administered questionnaire was used for this research. The study’s total population consisted of 2,762 women. The results revealed that the female students and employees at Taif University were influenced by five motivations (psychological motives, fitness-related motives, health motives, athletic-inclination motives, and social motives). Psychological motives were the highest -ranked items for the motivation elements that inspire adult Saudi women to participate in physical activity (AP). Concerning the challenges with PA practices, the results confirmed that the physical and service obstacles were the major constraints of daily physical-activity practices. The study indicated that 30-40-year-old women were more motivated to practice regular physical activity than the other participants. More empirical study is needed to confirm, refute, or interpret the conclusions.
Health Literacy Levels and Self-Rated Health in the state of Delaware: a cross-sectional Study
Authors: Tutu Raymond , Ameyaw Edmund , Kwagyan John , Ottie-Boakye Doris 
Published: November 2024

Abstract

Background
Better health literacy has been found to be associated with better health outcomes across varied populations. This study aimed to (1) examine the health literacy levels of individuals with respect to the extent to which they acquire, understand, appraise, and apply health information, and (2) to assess the relationship between health literacy and self-reported health in the state of Delaware.
Methods
The sampling frame consisted of 60 purposively selected faith-based organizations across the state of Delaware who predominantly serve minorities. Accounting for clustering design, a sample size was determined based on a Mixed Methods test. In total, 1095 participants responded to a survey questionnaire which included a validated short version of the European Health Literacy Questionnaire (HLS-EU-Q16). The data was analyzed using descriptive statistics, reliability techniques, chi-square test of independence, and ordinal logistic regression.
Results
The study found that about a quarter of the participants had inadequate level of health literacy and about another quarter had problematic level of health literacy. These results are reflected across the health domains – health care, disease prevention, and health promotion. Health literacy was predictor of self-rated health. The odds of better self-rated health are 0.360 times lower for people with inadequate health literacy levels compared to those with sufficient health literacy.
Conclusion
Health literacy profoundly shapes health outcomes. The high proportion of people expressing difficulty concerning accessing, comprehending, appraising, and using health information in the health care, disease prevention, and health promotion domains in state of Delaware would require a concerted effort.
Regional Earthquake Likelihood Models (RELMs) for Probabilistic Seismic Hazard Analysis (PSHA) around The Southern off-coast of Sumatra - West Java
Authors: Triyoso Wahyu 
Published: November 2024

Abstract

This study investigates the application of time-independent models for the Regional Earthquake Likelihood Models (RELMs) earthquake forecast testing based on the modified probability gain (mG) for seismic hazard study and analysis (SHA) in the southern off-coast of Sumatra-West Java utilizing a 15 years Region Time Length (RTL). This study used a declustered catalog of shallow earthquakes, dividing it into two observation periods: 1978–1992 and 1993-June 2006. Two rate models were developed to estimate earthquake occurrence probability for a given magnitude and time window. The normalized probability gain between these two observation periods was then calculated, leading to the mG estimation. The mG, in turn, provides insights into seismicity patterns for the SHA. The SHA model was constructed using a uniform background seismicity rate model based on the declustered earthquake with Mw ≥ 5.0, weighted by the mG. The Peak Ground Acceleration (PGA) vs. exceedance probability over 50 years was estimated and evaluated for several sites. They are Bengkulu, Liwa, and Kota Agung. They incorporated an amplification factor derived from the horizontal-vertical Spectral Ratio method, which allows for the estimation of surface PGA values. This research suggested a better understanding of future seismic hazards and informs mitigation efforts before large earthquakes.
White Light-Emitting Electrochemical Cells Based on Metal-Free TADF Emitters
Authors: Edman Ludvig , Tang Shi , Tsuchiya Youichi , Wang Jia , Adachi Chihaya 
Published: November 2024

Abstract

The attainment of broadband white emission from a light-emitting electrochemical cell (LEC) is important, since white light enables illumination and facile color conversion and because LEC devices can be cost-efficient, conformable and sustainable. However, a drawback with current white-emitting LECs is that they either employ non-sustainable metals as a critical emitter constituent or are intrinsically efficiency limited by that the emitter only converts singlet (and not triplet) excitons to photons. Organic compounds that emit by the process of thermally activated delayed fluorescence (TADF) can address these issues since they can harvest all the electrically generated excitons for light emission while being metal free. Here, we report on the first white LEC that utilizes solely metal-free TADF compounds for the emitting species. This was accomplished through systematic design, investigation and tuning of the energy-transfer processes and the electrochemically formed doping structure within the metal-free LEC active material, which comprises two color-complementary blue and orange TADF emitters, a blend host and an ionic-liquid electrolyte. The tuned TADF-LEC emits white light with a high color rendering index of 88 and CIE coordinates of (0.36, 0.38), and this broadband emission, which can be delivered at an external quantum efficiency of 2.11% and a luminance of 350 cd/m2, is demonstrated to be highly invariant to both viewing angle, operational time and current density.
A Novel Approach to Detect CD4 T-Lymphocytes Using a Microfluidic Chip and Compact Signal Processing Circuit
Authors: Huong Phi Thi , Phu Nguyen Van , Thanh Pham Van , Huy Hoang Van , Quynh Luu Manh , Thien Nguyen Duy , Huong Pham Thi Thu , Van Nguyen Thi Thanh , Luong Nguyen Hoang , Hong Tran Thi , Nam Nguyen Hoang 
Published: November 2024

Abstract

CD4 T-lymphocytes (CD4 cells) are a type of T lymphocyte that plays an important role in the immune system, helping to fight germs and protect the body from disease. Accurate enumeration of CD4 T cells is crucial for assessing immune health and diagnosing various diseases. This study presents the development and validation of a novel microfluidic biochip system designed for the detection and counting of CD4 T cells using impedance measurements. The proposed system integrated a cell detection chip with a cost-effective signal processing circuit, which included an instrumental amplifier and a highly sensitive lock-in amplifier. The sensing structure, created using advanced microfabrication technology, consists of three microelectrodes and a 50 µm × 50 µm measurement aperture. The detection principle relied on the impedance imbalance caused by the presence of CD4 T cells in the fluidic flow between adjacent sensing electrodes. The system's performance was validated through extensive experiments, demonstrating high accuracy in detecting and counting CD4 T cells separated from whole blood based on their magnetic properties. The experimental results indicate that the proposed system was simpler, faster, and more cost-effective compared to traditional laser flow cytometry. Furthermore, the system’s portability and ease of use made it highly suitable for point-of-care diagnostics and on-site cell analysis. The utilization of microfabrication technology and impedance measurement not only enhanced efficiency and accuracy but also offered a reliable solution for rapid biological cell detection. Future work will focus on enhancing the throughput and miniaturizing the sensing structure to align with the high standards of conventional flow cytometry while maintaining cost-effectiveness and simplicity. This research lays a solid foundation for the development of advanced lab-on-a-chip technologies for biological cell detection and analysis, promising significant improvements in healthcare diagnostics and monitoring.