bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glutathione deficiency induces epigenetic alterations of vitamin D metabolism genes in the livers of high-fat diet-fed obese mice
Authors: Parsanathan Rajesh , Jain Sushil K. 
Published: October 2019

AbstractObesity has been correlating with low levels of glutathione (GSH) and 25-hydroxyvitamin D3 (25(OH)VD3). The liver is the principal site for the 25(OH)VD3 biosynthesis. This study investigated whether GSH deficiency induces epigenetic alterations that impair Vitamin D (VD) metabolism genes in the livers of HFD-fed mice. The expression of the VD metabolism genes CYP2R1 and CYP27A1 (25-hydroxylase), CYP27B1 (1-α-hydroxylase), and vitamin D receptor (VDR) were downregulated in the livers of mice fed an HFD (GSH- deficient) compared with control diet-fed group. The expression of CYP24A1 (24-hydroxylase) was significantly increased, which catabolizes both 25(OH)VD3 and 1α,25-hydroxyvitaminD3. Gene-specific hypermethylation of 25-hydroxylase, 1-α-hydroxylase, and VDR, and hypomethylation of CYP24A1 was observed in HFD-fed mice. GSH deficiency induced in cultured hepatocytes caused an increase in oxidative stress and alterations in VD regulatory genes. Similarly, elevated global DNA methylation, Dnmt activity, and 5-methylcytosine but decreased Tet activity and 5-hydroxymethylcytosine were observed in the GSH-deficient hepatocytes and the liver of HFD-fed mice. Replenishment of GSH by its prodrugs treatment beneficially altered epigenetic enzymes, and VD-metabolism genes in hepatocytes. HFD-induces GSH deficiency and epigenetically alters VD-biosynthesis pathway genes. This provides a biochemical mechanism for the VD-deficiency and potential benefits of GSH treatment in reducing 25(OH)VD3-deficiency.
Ginsenoside Rg1 attenuates isoflurane/surgery-induced cognitive disorders and sirtuin 3 dysfunction
Authors: Miao Hui-Hui , Wang Min , Wang Hai-Xia , Tian Ming , Xue Fu-Shan 
Published: October 2019

Abstract
Isoflurane/surgery (I/S) may induce neurocognitive disorders, but detailed mechanisms and appropriate treatment remain largely unknown. This experiment was designed to determine whether ginsenoside Rg1 could attenuate I/S-induced neurocognitive disorders and Sirtuin3 (Sirt3) dysfunction. C57BL/6J male mice received 1.4% isoflurane plus abdominal surgery for 2 h. Ginsenoside Rg1 10 mg/kg was intraperitoneally given for 8 days before surgery. Neurocognitive function was assessed by the Barnes Maze test. Levels of reactive oxygen species (ROS), oxygen consumption rate (OCR), mitochondrial membrane potential (MMP), expression and deacetylation activity of Sirt3 in the hippocampus tissues were measured. Results showed that I/S induced hippocampus-dependent learning and memory impairments, with increased ROS levels, and reduced OCR, MMP, and expression and deacetylation activity of Sirt3 in hippocampus tissues. Ginsenoside Rg1 treatment before I/S intervention significantly ameliorated learning and memory performance, reduced ROS levels and improved the OCR, MMP, expression and deacetylation activity of Sirt3. In conclusion, this experiment demonstrates that ginsenoside Rg1 treatment can attenuate I/S-induced neurocognitive disorders and Sirt3 dysfunction.
BCYRN1 is correlated with progression and prognosis in gastric cancer
Authors: Zhai Hongbing , Li Yanju 
Published: October 2019

Abstract
Long non-coding RNA brain cytoplasmic RNA 1 (BCYRN1) has been found to play an important role in tumorigenesis of a variety of tumors including gastric cancer (GC). However, the prognostic significance and molecular mechanism of BCYRN1 was still unknown in GC. In the present study, we found BCYRN1 expression was dramatically elevated in GC tissues and cell lines, and positively associated with tumor depth, lymph node metastasis and clinical stage in patients with GC. Moreover, univariate and multivariate Cox regression analyses demonstrated that high BCYRN1 expression was independent prognostic factor for overall survival in GC patients. In lncRNA-microRNA interactome database, we found that there were putative binding sites between BCYRN1 and miR-204-5p. Furthermore, we confirmed that down-regulation of BCYRN1 inhibited GC cell proliferation, migration and invasion through directly up-regulated miR-204-5p expression. In conclusion, BCYRN1 acts as a promising prognostic predictor in GC patients and regulated GC cell proliferation, cell cycle, migration and invasion through targeting miR-204-5p.
Houshiheisan promotes angiogenesis via HIF-1α/VEGF and SDF-1/CXCR4 pathways: in vivo and in vitro
Authors: Xiang Yangyang , Yao Xiaoquan , Wang Xuan , Zhao Hui , Zou Haiyan , Wang Lei , Zhang Qiu-Xia 
Published: October 2019

Abstract
Rationale: Houshiheisan (HSHS), a classic prescription in traditional Chinese medicine (TCM), has remarkable efficacy in the treatment of ischemic stroke.
Objective: To investigate the pro-angiogenic effect and molecular mechanism of HSHS for stroke recovery.
Methods and results: The rat permanent middle cerebral artery occlusion (pMCAO) model was constructed by suture method, HSHS (5.25 or 10.5 g/kg) and Ginaton (28 mg/kg) treatment was intragastrically administrated at 6 h after modeling which remained for 7 consecutive days. Pathological evaluation conducted by Hematoxylin–Eosin (HE) staining and the results showed that HSHS alleviated blood vessel edema, reduced the damage to blood vessels and neurons in the ischemic areas. Immunostaining, quantitative real-time fluorescence PCR results showed that HSHS up-regulated pro-angiogenic factors including platelet endothelial cell adhesion molecule-1 (cluster of differentiation 31 (CD31)), vascular endothelial growth factor (VEGF), vascular endothelial growth factor A (VEGFA), VEGF receptor 2 (VEGFR2), angiopoietin-1 (Ang-1), while down-regulated angiopoietin-2 (Ang-2), stromal cell derived factor-1 (SDF-1), and cxc chemokine receptor 4 (CXCR4) expression in infarct rat cortex, and similar results were obtained in subsequent Western blot experiment. Furthermore, CCK8 assay and transwell migration assay were performed to assess cell proliferation, migration, and tube formation. The medicated serum (MS) of HSHS appeared to have beneficial effects for immortalized human umbilical vein cells (Im-HUVECs) on proliferation and migration after persistence hypoxia. Western blot analysis revealed that the expression of hypoxia inducible factor-1α (HIF-1α), VEGFA, Ang-1, Ang-2, and CXCR4 were significantly up-regulated while Ang-2 was down-regulated by HSHS MS treatment compared with vehicle group in vitro.
Conclusion: The present study suggests a novel application of HSHS as an effective angiogenic formula for stroke recovery.
Auswirkungen der Digitalisierung auf Sexualität und Beziehung
Authors: Melzer Heike 
Published: October 2019

ZUSAMMENFASSUNG
Gegenstand und Ziel Die digitale Transformation verändert die Sexualität und Beziehungsgestaltung vor allem junger Menschen in vielfältiger Weise.
Material und Methoden Klinische Beobachtungen aus der Praxis der Paar- und Sexualtherapie werden vor dem Hintergrund aktueller Forschungsergebnisse dargestellt.
Ergebnisse Es zeigt sich ein Anstieg sexueller Funktionsstörungen (erektile Dysfunktion, Hemmung oder Ausbleiben des Orgasmus und partnerbezogene Lustlosigkeit) besonders bei jungen Männern. Zudem entstehen zwanghafte oder süchtige Verhaltensweisen um Pornografie und Sexualität sowie sexuelle Abweichungen (Paraphilien) nehmen zu.
Schlussfolgerungen Der problematische und frühzeitige Konsum von starken sexuellen Stimuli (Pornografie, ständig wechselnde sexuelle Partner sowie leistungsstarke Sex-Toys) verursacht neue Probleme und Krankheitsbilder, die nicht genügend erforscht sind. Für wissenschaftlich fundierte präventive, diagnostische und therapeutisch interdisziplinäre Maßnahmen ist diese Forschung dringend erforderlich.
Klinische Relevanz Zur Förderung sexueller Gesundheit und Zufriedenheit müssen neue sexuelle Störungen bzw. problematische Erlebnis- und Verhaltensweisen im digitalen Kontext frühzeitig fachgerecht erkannt, diagnostiziert und therapiert werden.
Psychologische Behandlungsverfahren bei Kopfschmerz
Authors: Dresler Thomas , Klan Timo , Liesering-Latta Eva , Lüpke Johanna , Hubalek Irina , Kraya Torsten , Kropp Peter 
Published: October 2019

ZUSAMMENFASSUNGIn diesem Beitrag wird beschrieben, welche psychologischen Behandlungsverfahren in der Therapie von Kopfschmerzerkrankungen eingesetzt werden können. Technologische Entwicklungen in den letzten Jahrzehnten habe neue Möglichkeiten eröffnet, deren Potenzial es allerdings noch genauer auszuloten gilt. Die Idee moderner Kopfschmerztherapie ist nicht der isolierte Einsatz einzelner Verfahren, sondern eine möglichst gute, individuell auf den Patienten zugeschnittene Kombination verschiedener psychologischer Behandlungsverfahren mit anderen medikamentösen und nicht medikamentösen Verfahren. Um für jeden Kopfschmerzpatienten eine maßgeschneiderte Therapie zu ermöglichen, sollte ein interdisziplinäres Vorgehen das Ziel sein.
The Effects of Co-prescription of Pantoprazole on the Clozapine Metabolism
Authors: Kuzin Maxim , Schoretsanitis Georgios , Haen Ekkehard , Dammann Gerhard , Hiemke Christoph , Gründer Gerhard , Paulzen Michael 
Published: October 2019

Abstract
Background Polypharmacy including somatic medications such as proton pump inhibitors is a common phenomenon in psychiatric care. The aim of this study was to evaluate the pantoprazole effects on clozapine metabolism.
Methods A large therapeutic drug-monitoring database containing plasma concentrations of CLZ was analyzed. The results were stratified into four groups: a non-smoking (n=250) and a smoking group (n=326), and two groups co-medicated with pantoprazole: non-smokers (n=26) and smokers (n=29). The analysis was based on the non-parametrical Mann-Whitney U test (M-W-U) with a significance level of 0.05.
Results Differences reached statistical significance for pharmacokinetic parameters between CLZ monotherapy and co-medication with pantoprazole neither in smokers nor in non-smokers (p>0.05 for M-W-U in pairwise comparisons). In patients with clozapine monotherapy, smokers had a higher daily dosage of CLZ compared to non-smokers (mean dosage 363±181 vs. 291±145 mg/day, p<0.001 for M-W-U).
Conclusions Adding pantoprazole to an ongoing treatment with clozapine does not alter the metabolism of clozapine to a significant extent.
Thoracic Renal Artery
Authors: Rossi Umberto G. , Rollandi Gian Andrea 
Published: October 2019

AbstractWe report a case of right renal artery originating from the thoracic aorta with normal position of the right kidney. Although this anomaly is rare, vascular, thoracic, and urological surgeons should be aware about this vascular variant before planning their surgical intervention.
Clinical Characteristics of Patients with Trigeminal Neuralgia Referred to Neurosurgery
Authors: Siqueira Silvia RDT , Teixeira Manoel J , Siqueira José TT 
Published: October 2019

ABSTRACTObjectives: To investigate the clinical characteristics of patients with trigeminal neuralgia referred to surgery in a center of reference.Methods: We evaluated the general characteristics of 395 patients with trigeminal neuralgia referred to neurosurgery as treatment. They corresponded to 2 samples of 1984 and 2004. The EDOFHC protocol (Orofacial Pain Questionnaire) and the medical profile were used.Results: In the first study (1984), with 290 patients, the higher prevalence was: women (57.3%), white (95.5%), with mean age of 62.5. The most affected trigeminal branches were the maxillary and / or mandibular branches (65.5%), and the right side was the most affected (57.6%). From the second study (2004), with 105 patients, 57.1% were women, 75.2% white, with a mean age of 60.8. The maxillary and / or mandibular branches (79.0%) and the right side (69.5%) were the most affected. Both samples had neurological abnormalities and systemic diseases (mainly cardiovascular).Conclusions: General characteristics of these patients were similar to other samples of trigeminal neuralgia. Neurological findings were also present in patients with no previous surgical treatment for TN. Hypertension and cardiac diseases were also frequent and make the monitoring of the patients during crises necessary. (Eur J Dent 2009;3:207-212)
Perioperative Anemia Management
Authors: Abeysiri Sandaruwani , Chau Marisa , Richards Toby 
Published: October 2019

AbstractAnemia is increasingly recognized as an interventional hematological target in patients before major surgery. Preoperative anemia increases the need for perioperative blood transfusion, and there is now a well-recognized association with increased patient complications, length of hospital stay, and worse outcomes. Patient Blood Management (PBM) is a World Health Organization endorsed, evidence-based management bundle of care in transfusion hemostasis that focuses on three main aspects: anemia management, prevention of blood loss, and appropriate transfusion practice. Implementation of PBM guidelines has been accompanied by reduced transfusion needs and improved patient outcomes. In a patient presenting with preoperative anemia or at risk of transfusion, this should initiate a program of PBM that manages the patient through the entire operative period. We review the current evidence on the three pillars of PBM and highlight those aspects with the strongest evidence in support of their impact.
Evaluation of Cariogenic Bacteria
Authors: Nishikawara Fusao , Nomura Yoshiaki , Imai Susumu , Senda Akira , Hanada Nobuhiro 
Published: October 2019

ABSTRACTObjectives : The evaluation of Mutans streptococci (MS) is one of the index for caries risk. DentocultTM and CRTTM are commercial kits to detect and evaluate MS, conveniently. However, the evaluation of MS has also been carried out simply using an instruction manual. But the instruction manual is not easy to use for evaluation of MS. The aim of this study was to examine the utility of modified Mitis-Salivalius Bacitracin (MSB) agar medium compared with MSB agar medium and commercial kits, and to establish a convenient kit (mMSB-kit) using modified MSB agar.Methods : The MS in stimulated saliva from 27 subjects were detected by MSB, modified MSB agar medium and commercial kits. Laboratory and clinically isolated strains of MS were similarly evaluated. The ratios of MS in detected bacteria were compared by ELISA.Results : The scores using an mMSB-kit on the basis of modified MSB agar medium were tabulated. Saliva samples showed different levels of MS between culture methods and the commercial kit. Some samples which were full of MS were not detected by the commercial kit. The detection of MS by modified MSB agar medium and mMSB-kit were significantly higher when compared with MSB agar medium, CRTTM, (P< .01) and Dentocult SMTM (P<.05).Conclusion : The sensitivity for detection of MS is higher for modified MSB agar medium when compared with MSB agar medium. The mMSB-kit can be used simply, and can be an important contributor for the evaluation of MS as a caries risk factor. (Eur J Dent 2007;1:31-39)
The Effect of Luting Agents and Ceramic Thickness on the Color Variation of Different Ceramics against a Chromatic Background
Authors: Azevedo Cubas Gloria Beatriz de , Camacho Guilherme Brião , Demarco Flávio Fernando , Pereira-Cenci Tatiana 
Published: October 2019

ABSTRACTObjectives: The aim of this study was to assess the influence of various ceramic thicknesses and luting agents on color variation in five ceramic systems. Methods: Fifteen disc-shaped ceramic specimens (11 mm diameter; shade A3) were fabricated with each of the six veneering ceramics tested, with 1, 1.5, or 2 mm thickness (n=5). Resin composite discs (Z-250, shade C4) were used as bases to simulate a chromatic background. The cementation of the veneers was carried out with an opaque resin-based cement (Enforce, shade C4), a resin-based cement (Enforce, shade A3), or without cement (C4, control group). Color differences (ΔE*) were determined using a colorimeter. Three-way ANOVA was used to analyze the data, followed by a Tukey post-hoc test (α=.05). Results: The L*a*b* values of the ceramic systems were affected by both the luting agent and the ceramic thickness (P<.05). In general, there was no difference between the control group and the group using the resin-based cement. The use of an opaque luting agent resulted in an increase of the color coordinates a*, b*, L*, producing differences in ΔE* values for all ceramics tested, regardless of the thickness (P<.05). For the 2-mm thick veneers, higher values in the color parameters were obtained for all ceramics and were independent of the luting agent used. Conclusions: The association of 2-mm thickness with opaque cement presented the strongest masking ability of a dark colored background when compared to a non-opaque Lutinging agent and the other thicknesses tested. (Eur J Dent 2011;5:245-252)
Effect of Occlusal Splints on the Temporomandibular Disorders, Dental Wear and Anxiety of Bruxist Children
Authors: Restrepo Claudia C , Medina Isabel , Isabel Patiñob 
Published: October 2019

ABSTRACTObjectives: To evaluate the effectiveness of occlusal splints to reduce the signs and symptoms of temporomandibular disorders (TMD), dental wear and anxiety in a group of bruxist children. Methods: All of the subjects were 3 to 6 years old, had complete primary dentition, class I occlusion and were classified as bruxist according to the minimal criteria of the ICSD for bruxism. For each child, anxiety was evaluated with the Conners’ Parent Rating Scales (CPRS). The TMD were evaluated using the RDC/TMD. The dental wear was processed in digital format with Mat Lab® and Lab view® software to determine its size and form. The children were randomized into an experimental (n=19) and a control (n=17) group. The children in the experimental group used rigid bite plates for a two-year period, until mixed dentition. Afterwards, the CPRS and the RDC/TMD were applied again and dental casts were taken. Comparisons of the variables regarding dental wear, signs and symptoms of TMD and anxiety before and after treatment among the groups were analyzed using the t-test, the Wilcoxon rank sum test and the Mann-Whitney test. Results: The subjects in the experimental group showed no statistically significant difference regarding anxiety levels and dental wear when compared with the control group. The signs and symptoms of TMD were not reduced except for the deviation in mouth opening. Conclusions: The use of rigid occlusal bite plates was not efficient in reducing the signs of bruxism as a whole but did reduce the deviation in mouth opening. (Eur J Dent 2011;5:441-450)
Prevention of demineralization around orthodontic brackets using two different fluoride varnishes
Authors: Nalbantgil Didem , Oztoprak Mehmet Oguz , Cakan Derya Germec , Bozkurt Kemal , Arun Tulin 
Published: October 2019

ABSTRACTObjective: This parallel controlled clinical trial evaluated the effect of a gel containing Lippia sidoides essential oil on plaque and gingivitis control.Methods: Thirty patients (n=30) were randomly selected and allocated into three groups: Lippia sidoides (LS, n=10), chlorhexidine (CLX, n=10) or placebo (control, n=10). Plaque and bleeding index were recorded at baseline and after three months. All volunteers were instructed to brush with the gel three times a day throughout the experiment period.Results: There was a significant reduction on plaque and gingivitis in the test groups (P<.05), but no statistically significant difference was observed between them (P<.05).Conclusion: A gel preparation containing 10% Lippia sidoides essential oil was an efficient herbal antiplaque and antigingivitis agent. (Eur J Dent 2013;7:28-34)
The spatial competition between containerised rail and sea transport in Eurasia
Authors: Lu Mengqiu , Chen Yu , Morphet Robin , Lu Yuqi , Li Enkang 
Published: October 2019

AbstractThe competition in space between rail and sea transport is of great significance to the integration of Eurasia. This paper proposes a land and sea transport spatial balance model for container transport, which can extract a partition line on which transport costs by rail and sea are equal given a destination. Four scenarios are discussed to analyse the effects of different factors on the model. Then the model is empirically tested on current rail and sea transport networks to identify the transport competition pattern in Eurasia. The location of destinations, the freight costs, and time costs are the three main factors affecting the model. Among them, time costs are determined by the value of a container and its contents, the interest rate, and by time differences between land and sea transport. The case study shows that Eurasia forms a transport competition pattern with a land area to sea area ratio of about 1:2; this ratio, however, changes to 1:1 when time costs are considered. Further, the land and sea transport balance lines are consistent with the theories of geopolitics, which indicate that the same processes may exist in the spatial pattern of geo-economics and geopolitics in Eurasia. According to the balance lines, we get a spatial partition, dividing Eurasia into the land transport preferred area, the land–sea transport indifference area, and the sea transport preferred area. The paper brings a new perspective to the exploration of geopolitical economic spatial patterns of Eurasia and provides a practical geographic theory as an analytic basis for the implementation of the Belt and Road Initiative.
A generalized theorem on curvilinear three-web boundaries and its applications
Authors: Shelekhov A. M. 
Published: October 2019

Abstract
Suppose that a curvilinear three-web is given by the equation

. A specific structure of the derivatives of the function

is established that characterizes regular three-webs. This makes it possible to list all regular three-webs formed by the Cartesian net and a family of circles, and also by the Cartesian net and a family of second-order curves.
Bibliography: 4 titles.
Integrable billiard systems realize toric foliations on lens spaces and the 3-torus
Authors: Vedyushkina V. V. 
Published: October 2019

Abstract
An integrable billiard system on a book, a complex of several billiard sheets glued together along the common spine, is considered. Each sheet is a planar domain bounded by arcs of confocal quadrics; it is known that a billiard in such a domain is integrable. In a number of interesting special cases of such billiards the Fomenko-Zieschang invariants of Liouville equivalence (marked molecules

) turn out to describe nontrivial toric foliations on lens spaces and on the 3-torus, which are isoenergy manifolds for these billiards.
Bibliography: 18 titles.
The deaminase APOBEC3B triggers the death of cells lacking uracil DNA glycosylase
Authors: Serebrenik Artur A. , Starrett Gabriel J. , Leenen Sterre , Jarvis Matthew C. , Shaban Nadine M. , Salamango Daniel J. , Nilsen Hilde , Brown William L. , Harris Reuben S. 
Published: October 2019

Significance
APOBEC3B (A3B) is overexpressed in multiple tumor types and a major source of mutation by converting DNA cytosines to uracils. A3B-positive tumors are often more heterogenous and result in poor clinical outcomes. We show that a proportion of A3B-catalyzed uracil lesions is repaired by uracil DNA glycosylase 2 (UNG)-driven base excision repair and that rendering cells UNG-defective leads to cytotoxicity. This cell death mechanism requires uracil processing by mismatch repair proteins and functional p53. Our results indicate that UNG inhibition could be a strategy to kill a subset of A3B-positive tumors.