bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous determination of 10 kinds of biogenic amines in rat plasma using high‐performance liquid chromatography coupled with fluorescence detection
Authors: Wang Xinna , Liang Yawei , Wang Yaqi , Fan Mingqin , Sun Yanni , Liu Jianli , Zhang Ning 
Published: February 2018

AbstractWe describe a simple, rapid, selective and sensitive HPLC method coupled with fluorescence detection for simultaneous determination of 10 kinds of biogenic amines (BAs: tryptamine, 2‐phenethylamine, putrescine, cadaverine, histamine, 5‐hydroxytryptamine, tyramine, spermidine, dopamine and spermine). BAs and IS were derivated with dansyl chloride. Fluorescence detection (λex/λem = 340/510 nm) was used. A satisfactory result for method validation was obtained. The assay was shown to be linear over the ranges 0.005–1.0 μg/mL for tryptamine, 2‐phenethylamine and spermidine, 0.025–1.0 μg/mL for putrescine, 0.001–1.0 μg/mL for cadaverine, 0.25–20 μg/mL for histamine, 0.25–10 μg/mL for 5–hydroxytryptamine and dopamine, and 0.01–1.0 μg/mL for tyramine and spermine. The limits of detection and the limits of quantification were 0.3–75.0 ng/mL and 1.0–250.0 ng/mL, respectively. Relative standard deviations were ≤5.14% for intra‐day and ≤6.58% for inter‐day precision. The recoveries of BAs ranged from 79.11 to 114.26% after spiking standard solutions of BAs into a sample at three levels. Seven kinds of BAs were found in rat plasma, and the mean values of tryptamine, 2‐phenethylamine, putrescine, cadaverine, histamine, spermidine and spermine determined were 52.72 ± 7.34, 11.45 ± 1.56, 162.56 ± 6.26, 312.75 ± 18.11, 1306.50 ± 116.16, 273.89 ± 26.41 and 41.51 ± 2.07 ng/mL, respectively.
Light‐Dependent Cytoplasmic Recruitment Enhances the Dynamic Range of a Nuclear Import Photoswitch
Authors: Yumerefendi Hayretin , Wang Hui , Dickinson Daniel J. , Lerner Andrew M. , Malkus Per , Goldstein Bob , Hahn Klaus , Kuhlman Brian 
Published: February 2018

AbstractCellular signal transduction is often regulated at multiple steps to achieve more complex logic or precise control of a pathway. For instance, some signaling mechanisms couple allosteric activation with localization to achieve high signal to noise. Here, we create a system for light‐activated nuclear import that incorporates two levels of control. It consists of a nuclear import photoswitch, light‐activated nuclear shuttle (LANS), and a protein engineered to preferentially interact with LANS in the dark, Zdk2. First, Zdk2 is tethered to a location in the cytoplasm that sequesters LANS in the dark. Second, LANS incorporates a nuclear localization signal (NLS) that is sterically blocked from binding to the nuclear import machinery in the dark. If activated with light, LANS both dissociates from its tethered location and exposes its NLS, which leads to nuclear accumulation. We demonstrate that this coupled system improves the dynamic range of LANS in mammalian cells, yeast, and Caenorhabditis elegans and provides tighter control of transcription factors that have been fused to LANS.
Prognostic value of the index of microcirculatory resistance after percutaneous coronary intervention in patients with non‐ST‐segment elevation acute coronary syndrome
Authors: Murai Tadashi , Yonetsu Taishi , Kanaji Yoshihisa , Usui Eisuke , Hoshino Masahiro , Hada Masahiro , Hamaya Rikuta , Kanno Yoshinori , Lee Tetsumin , Kakuta Tsunekazu 
Published: February 2018

AbstractBackgroundThe prognostic value of physiological indices in non‐ST‐segment elevation acute coronary syndrome (NSTE‐ACS) patients undergoing percutaneous coronary intervention (PCI) is unknown. We investigated the prognostic efficacy of physiological indices obtained after PCI in patients with NSTE‐ACS.MethodsEighty‐three patients (men: n = 70, age: 63.7 ± 9.7 years) undergoing PCI for NSTE‐ACS within 48 hr postadmission were investigated. Fractional flow reserve (FFR), coronary flow reserve (CFR), and the index of microcirculatory resistance (IMR) of the culprit vessels were measured after the completion of PCI. The patients were clinically followed up to determine major cardiac adverse events (MACE), including death, congestive heart failure requiring hospitalization, and remote coronary revascularization.ResultsThe median FFR, CFR, and IMR values were 0.90 (interquartile range [IQR] 0.86–0.95), 2.38 (IQR 1.75–4.17), and 22.9 (IQR 11.2–31.5), respectively. During a median follow‐up of 20.7 months, 19 MACEs (22.9%) were documented. No significant difference in baseline patient characteristics, except for age, was detected between patients with and without MACE. Patients with MACE showed higher IMR and lower CFR than those without (IMR: 27.2 vs. 16.3; P = 0.001, CFR: 1.82 vs. 2.55; P = 0.04), whereas FFR was not significantly different (0.92 vs. 0.89; P = 0.72), irrespective of the MACE occurrence. Post‐PCI IMR was the only independent predictor of MACE (hazard ratio 1.033, 95% confidence interval 1.013–1.052, P = 0.001). The MACE‐free survival was significantly worse in patients with high post‐PCI IMR (χ2 7.12; P = 0.008).ConclusionPost‐PCI IMR may help identify patients at high risk for subsequent adverse coronary events who require adjunctive therapeutic strategies.
α‐Hydroxy Ketones as Masked Ester Donors in Brønsted Base Catalyzed Conjugate Additions to Nitroalkenes
Authors: Olaizola Iurre , Campano Teresa E. , Iriarte Igor , Vera Silvia , Mielgo Antonia , García Jesús M. , Odriozola José M. , Oiarbide Mikel , Palomo Claudio 
Published: February 2018

AbstractThe catalyst‐controlled enantioselective direct addition reaction of enolizable esters and related carboxylic acid derivatives to π electrophiles remains a difficult synthetic transformation. In this study, the suitability of α‐hydroxy ketones as ester equivalents capable of being activated by bifunctional Brønsted base catalysts in the context of conjugate addition reactions to nitroolefins is demonstrated. The scope of the reaction, which affords the corresponding Michael adducts with very high stereoselectivity (diastereomeric ratio (d.r.) ≥95:5, up to 99 % enantiomeric excess (ee)), and its limitations are explored, as is the aftermath elaboration of adducts into densely functionalized enantioenriched products.
Experimental and Theoretical Identification of the Origin of Magnetic Anisotropy in Intermediate Spin Iron(III) Complexes
Authors: Wang Lianke , Zlatar Matija , Vlahović Filip , Demeshko Serhiy , Philouze Christian , Molton Florian , Gennari Marcello , Meyer Franc , Duboc Carole , Gruden Maja 
Published: February 2018

AbstractThe complexes [FeLN2S2X] [in which LN2S2=2,2′‐(2,2′‐bipryridine‐6,6′‐diyl)bis(1,1′‐diphenylethanethiolate) and X=Cl, Br and I], characterized crystallographically earlier and here (Fe(L)Br), reveal a square pyramidal coordinated FeIII ion. Unusually, all three complexes have intermediate spin ground states. Susceptibility measurements, powder cw X‐ and Q‐band EPR spectra, and zero‐field powder Mössbauer spectra show that all complexes display distinct magnetic anisotropy, which has been rationalized by DFT calculations.
A Step into the Future: Applications of Nanoparticle Enzyme Mimics
Authors: Korschelt Karsten , Tahir Muhammad Nawaz , Tremel Wolfgang 
Published: February 2018

AbstractWe describe elementary concepts, up‐to‐date developments, and perspectives of the emerging field of nanoparticle enzyme mimics (so‐called “nanozymes”) at the interface of chemistry, biology, materials, and nanotechnology. The design and synthesis of functional enzyme mimics is a long‐standing goal of biomimetic chemistry. Metal complexes, polymers and engineered biomolecules capturing the structure of natural enzymes or their active centers have been made to achieve high rates and enhanced selectivities. Still, the design of new “artificial enzymes” that are not related to proteins but with capacity of production and stability at industrial level, remains a goal. Inorganic nanoparticles bear this potential. Although it seems counterintuitive to compare nanoparticles and natural enzymes because they appear very different they share many common features: nano‐size, irregular shape, and rich surface chemistry. These features enable nanomaterials to mimic reactions of natural enzymes. Representative examples with biomedical and environmental applications are given.
Assessing Die Filling Using High‐Speed Camera Imaging
Authors: Hildebrandt Claudia , Loerzer Jost , Gopireddy Srikanth R. , Urbanetz Nora 
Published: February 2018

AbstractThe study presents the die filling process of pharmaceutical powders using high‐speed camera imaging. The objectives are to qualitatively visualize and to quantify the powder flow. A model die filling system with a shoe for powder storage and a moving die underneath the shoe was designed. The die filling process is captured through a high‐speed camera and the images allow flow type identification and flow rate calculation. Correlations are generated to predict the fill ratios and flow rates based on particle size data, which could help in process understanding and optimization of die filling.
Evaluation of Processes for Mechanical Manufacturing of Composite Materials for Li‐Sulfur Batteries
Authors: Titscher Paul , Zellmer Sabrina , Burmeister Christine F. , Schmidt Lars O. , Breitung‐Faes Sandra , Garnweitner Georg , Kwade Arno 
Published: February 2018

AbstractLithium‐sulfur batteries are a promising alternative to lithium‐ion batteries due to their low weight and the high availability of sulfur. In addition to research on material aspects, scalable processes are needed to manufacture tailored sulfur cathodes with high‐energy densities. Therefore, this work focuses on the usage of mechanical processes to prepare defined carbon‐sulfur composite materials that are subsequently processed to electrodes. DEM simulations are used to calculate the necessary power input and the resulting stress energy for different mechanical processes.
How Well Do Owner–Managers Forecast Potential Future Bankruptcy? Assessing Fear of Failure Using Employment Insurance for SMEs
Authors: Pommet Sophie , Sattin Jean‐François 
Published: February 2018

AbstractThe literature so far has not addressed how owner–managers deal with the risks associated with potential future bankruptcy. We propose here a new way to assess the fear of failure experienced by SME owner–managers and examine whether these risks are being anticipated accurately, by focusing on the benefits to be gained from French unemployment insurance. We compare the valuation of the benefits of the unemployment insurance scheme for the two populations of insured and uninsured owner–managers after matching. We rely on matching procedures and a five‐step protocol. Our results show that owner–managers' awareness of possible future problems increases when becoming insured and after experiencing a failure, suggesting some business failure myopia in this population. Copyright © 2018 ASAC. Published by John Wiley & Sons, Ltd.
Attachment and therapeutic alliance in psychological therapy for people with recent onset psychosis who use cannabis
Authors: Berry Katherine , Palmer Tom , Gregg Lynsey , Barrowclough Christine , Lobban Fiona 
Published: February 2018

We examine associations between client attachment style and therapeutic alliance in a 3‐arm randomized controlled trial of brief motivational interviewing and cognitive–behavioural therapy compared with longer term motivational interviewing and cognitive–behavioural therapy or standard care alone. Client self‐report measures of attachment style were completed at baseline, and both clients and therapists in the treatment arms of the trial completed alliance measures 1 month into therapy. We found that insecure–anxious attachment was positively associated with therapist‐rated alliance, whereas clients with insecure–avoidant attachment were more likely to report poorer bond with therapist. There was no evidence that client attachment significantly predicted clinical or substance misuse outcomes either directly or indirectly via alliance. Nor evidence that the length of therapy offered interacted with attachment to predict alliance.
Improved protocols to accelerate the assembly of DNA barcode reference libraries for freshwater zooplankton
Authors: Elías‐Gutiérrez Manuel , Valdez‐Moreno Martha , Topan Janet , Young Monica R. , Cohuo‐Colli José Angel 
Published: February 2018

AbstractCurrently, freshwater zooplankton sampling and identification methodologies have remained virtually unchanged since they were first established in the beginning of the XX century. One major contributing factor to this slow progress is the limited success of modern genetic methodologies, such as DNA barcoding, in several of the main groups. This study demonstrates improved protocols which enable the rapid assessment of most animal taxa inhabiting any freshwater system by combining the use of light traps, careful fixation at low temperatures using ethanol, and zooplankton‐specific primers. We DNA‐barcoded 2,136 specimens from a diverse array of taxonomic assemblages (rotifers, mollusks, mites, crustaceans, insects, and fishes) from several Canadian and Mexican lakes with an average sequence success rate of 85.3%. In total, 325 Barcode Index Numbers (BINs) were detected with only three BINs (two cladocerans and one copepod) shared between Canada and Mexico, suggesting a much narrower distribution range of freshwater zooplankton than previously thought. This study is the first to broadly explore the metazoan biodiversity of freshwater systems with DNA barcodes to construct a reference library that represents the first step for future programs which aim to monitor ecosystem health, track invasive species, or improve knowledge of the ecology and distribution of freshwater zooplankton.
Comorbid depressive symptoms and self‐esteem improve after either cognitive‐behavioural therapy or family‐based treatment for adolescent bulimia nervosa
Authors: Valenzuela Fabiola , Lock James , Le Grange Daniel , Bohon Cara 
Published: February 2018

AbstractThis study examined the effect of family‐based treatment for bulimia nervosa (FBT‐BN) and cognitive behavioral therapy for adolescents (CBT‐A) on depressive symptoms and self‐esteem in adolescents with BN. Data were collected from 110 adolescents, ages 12–18, who met Diagnostic and Statistical Manual of Mental Disorders‐Fourth Edition, text revision criteria for BN or partial BN. Participants were randomly assigned to FBT‐BN or CBT‐A and completed measures of depressive symptoms and self‐esteem before and after treatment and at 6‐ and 12‐month follow‐up assessments. Depressive symptoms and self‐esteem significantly improved in both treatments, and neither treatment appeared superior on these clinical outcomes. Parents often worry whether FBT‐BN addresses comorbid depressive symptoms and low self‐esteem. Our findings address this concern, as they demonstrate that FBT‐BN does not differ from CBT‐A in improving depressive symptoms and self‐esteem, and both treatments result in symptom improvement. These findings can help clinicians guide families to choose a treatment that addresses BN and depressive symptoms and low self‐esteem.
Laboratory based continuous bedload monitoring in a model retention basin: Application of time domain reflectometry
Authors: Miyata Shusuke , Fujita Masaharu 
Published: February 2018

AbstractIn this study, we applied time domain reflectometry (TDR) to determine the deposition height and porosity of sediment at a fine spatiotemporal resolution, and developed a continuous bedload monitoring method that can be applied to pools in steep mountain rivers. The TDR monitoring system consisted of sensor probes, a cable tester, multiplexers and coaxial cables. When the embedded probes penetrated both water and sediment, the boundaries of the sediment and water were consistent with the transition points in the observed waveforms of each TDR measurement. A semi‐automatic analysis of the recorded TDR waveforms, which did not require calibration or parameter fitting, was conducted to establish continuous monitoring. In addition, a flume experiment was performed to test the monitoring system in a model retention basin connected to a flume, with sand of uniform grain size (1.4 mm diameter) supplied for 30 min. The sediment volume in the container representing the model basin was monitored using a load cell underlying the container and eight sensor probes, with a length of almost 0.27 m. The sediment thickness determined by the TDR indicated a gradual deposition, and was consistent with manual measurements. Despite a marginal overestimation of 13% for a sand feed of 30 kg, the sediment volume in the model retention basin and the bedload transport rate were successfully estimated. A combination of our monitoring system and other indirect methods, such as geophones, can potentially serve as useful tools for better understanding bedload transport processes in steep mountain streams. Copyright © 2018 John Wiley & Sons, Ltd.
Sydney Metro – ground characterisation and TBM selection for the Sydney Harbour crossing
Authors: Och David J. , Bateman Geoff , Maidl Ulrich , Comulada Marc 
Published: February 2018

AbstractThe Sydney Harbour crossing is a key element of Sydney Metro City & Southwest, which includes 15.5 km of twin tube running tunnels extending from Chatswood at the north through to Sydenham, south of the Central Buisness District (CBD). One of the key features is the 1 % length of the alignment that passes under Sydney Harbour. The rest of the alignment is through rock, but the harbour crossing is designed to pass through harbour sediments and mixed face conditions undersea. An in depth study and analysis was required to confirm the feasibility of the safe construction of this short length of the tunnel in sub‐aqueous, soft ground conditions. It was necessary to carry out detailed, targeted investigation of the ground to enable the selection of an appropriate tunnelling technique for constructing the tunnels.
Challenges and innovative solutions at the new construction of the Bözberg Tunnel
Authors: Zieger Thomas , Bühler Martin , Rick Beat , Schmid Werner , Grossauer Karl 
Published: February 2018

AbstractIn order to further increase the transfer of transalpine traffic through Switzerland from road to rail, the Gotthard axis is to be improved to a 4 m corridor. For this purpose altogether 270 km of railway line between Basel and the Italian border is to be improved to a corner height of 4 m by 2020. The largest individual structure is the new Bözberg Tunnel in the canton Aargau. Various options were investigated for the preliminary design. The best variant turned out to be the new construction of a two‐track tunnel parallel to the existing tunnel. The existing two‐track Bözberg Tunnel, which is not suitable for upgrading to the 4 m standard, will be used as a service and escape tunnel. Various emergency exits lead through five cross passages from the new to the old tunnel. The short construction time of five years until opening on the one hand, and the challenging geology in the Faltenjura with swelling rock mass on the other pose great problems for client, designers, and the contractor. This article reports on these challenges and describes how they have also led to innovative solutions.
How does retirement affect healthcare expenditures? Evidence from a change in the retirement age
Authors: Bíró Anikó , Elek Péter 
Published: February 2018

SummaryUsing individual‐level administrative panel data from Hungary, we estimate causal effects of retirement on outpatient and inpatient care expenditures and pharmaceutical expenditures. Our identification strategy is based on an increase in the official early retirement age of women, using that the majority of women retire upon reaching that age.According to our descriptive results, people who are working before the early retirement age have substantially lower healthcare expenditures than nonworkers, but the expenditure gap declines after retirement. Our causal estimates from a two‐part (hurdle) model show that the shares of women with positive outpatient care, inpatient care, and pharmaceutical expenditures, respectively, decrease by 3.0, 1.4, and 1.3 percentage points in the short run due to retirement. These results are driven by the relatively healthy, by those who spent some time on sick leave and by the less educated. The effect of retirement on the size of positive healthcare expenditures is generally not significant.
Novel calcitonin gene‐related peptide/chitosan‐strontium‐calcium phosphate cement: Enhanced proliferation of human umbilical vein endothelial cells in vitro
Authors: Lv Tiancheng , Liang Wei , Li Li , Cui Xu , Wei Xiaomou , Pan Haobo , Li Bing 
Published: February 2018

AbstractBone cement materials have some disadvantages, including slow degradation and no biological activity, which greatly weakens their clinical application. Therefore, the search for a multifunctional bioactive bone cement has become urgent. In this study, a novel bone cement sample of calcitonin gene‐related peptide (CGRP)/chitosan‐strontium (Sr)‐calcium phosphate cement (CPC) was developed. The structure and morphology were observed by scanning electron microscope (SEM). The cytotoxicity and proliferation of CGRP/chitosan‐Sr‐CPC were also measured. The expression of CGRP receptor 1 was measured using an immunofluorescence assay. Real‐time polymerase chain reaction (PCR) and enzyme‐linked immunosorbent assay (ELISA) were employed to quantify the VEGF mRNA and protein levels, respectively. Finally, the ability of the material to improve angiogenesis was assessed by using human umbilical vein endothelial cells (HUVECs) tube formation assay. The results showed that CGRP/Chitosan‐Sr‐CPC had the characteristics of a good orthopedic material without showing cell cytotoxicity to HUVECs. Meanwhile, CGRP/chitosan‐Sr‐CPC could release CGRP and enhance the proliferation of HUVECs via CGRP receptors. Moreover, CGRP/chitosan‐Sr‐CPC significantly upregulated the expression of the VEGF gene and protein in HUVECs, which might help improve the angiogenesis microenvironment. Besides, CGRP/chitosan‐Sr‐CPC could significantly improve angiogenesis of HUVECs. These findings provide new therapeutic material for the treatment of osteoporotic bone injury. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 19–28, 2019.