bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Extracellular Proteome of Staphylococcus aureus: A Mass Spectrometry based Proteomics Method of Exotoxin Characterisation
Authors: Das Rajdeep , D`souza Nisha , Choubey Surya K. , Murlidharan Sethumadhavan , Kurpad Anura V. , Mandal Amit K. 
Published: February 2019

Background:
Staphylococcus aureus (S. aureus), an important pathogen, causes a wide
range of infections in human starting from food poisoning to septicemia. It affects the host cells with
various exotoxins, known as virulence factors, which are synthesized in growth phase-dependent manner
of the bacteria. S. aureus has been reported to become resistant to antibiotics rapidly. Among two
common clinical isolates, Methicillin-sensitive S. aureus (MSSA) and Methicillin-resistant S. aureus
(MRSA), MRSA pose major problems across hospitals around the world.

Objective:
The objective of the present study was to profile the exoproteins of Methicillin-sensitive
S. aureus (ATCC 25293) and subsequently to establish a proteomics-based method of characterization
of S. aureus that is crucial in treating hospital-acquired infections.

Methods:
We used two-dimensional nanoLC/ESI-MS based proteomic platform to characterize and
quantify the exoproteins isolated from Methicillin-sensitive S. aureus (ATCC 25293) strain.

Results:
A total of 69 proteins were identified from extracellular proteome pool of ATCC 25293 strain
that includes 18 extracellular proteins, 40 cytoplasmic proteins, 2 membrane proteins, 3 cell wall proteins
and 6 uncharacterized proteins.

Conclusion:
We propose that this mass spectrometry-based proteomics method of characterization of
exoproteins might be useful to identify S. aureus strains that are resistant to antibiotics.
Development and Validation of HPLC-UV Method for the Determination of a Potent Synthetic Cannabinoid THJ-2201 in Mouse Plasma and Application in a Pharmacokinetic Study
Authors: Aboul-Enein Hassan Y. , Mostafa Gamal A.E , AlRabiah Haitham , Al-Ramadi Mohammed , Attia Sabry M. , Wagdy Hebatallah A. 
Published: February 2019

Aim:
A new simple and sensitive high-Performance Liquid Chromatography (HPLC) method
for the determination of a potent synthetic cannabinoid THJ-2201, has been developed and validated.
Lixiviptan was used as the Internal Standard (IS).

Methods:
THJ-2201 and IS were extracted from mouse plasma using deproteinization procedure that
uses acetonitrile followed by HPLC analysis. The separation was carried out on a reversed-phase C18
column using water and acetonitrile mixture (30:70 v/v). The flow-rate was 1.0 mL/min. Eluting of both
THJ-2201 and lixivaptan was performed at 220 nm.

Results:
The method demonstrated linearity over a calibration range of 95 - 1500 ng/mL and the Limit
of Detection (LOD) and Quantitation (LOQ) were 28 ng/mL and 91 ng/mL, respectively. The validation
of the proposed method was carried out by following the US Food and Drug Administration (FDA)
guidelines. Intra- and inter-day precision did not exceed 6.4%, whereas the accuracy of THJ-2201
measurements was within ±13%.

Conclusion:
This new method is simple and sensitive and has been applied successfully in a pharmacokinetic
study of THJ-2201 in mouse plasma. The mean values of Tmax and Cmax were 0.25 h and
141.87 ± 12.11 ng/mL, respectively.
Genetic Diversity of Blattella germanica Isolates from Central China based on Mitochondrial Genes
Authors: Wei Pan , Xie XiaoDong , Wang Ran , Zhang JianFeng , Li Feng , Luo ZhaoPeng , Wang Zhong , Wu MingZhu , Yang Jun , Cao PeiJian 
Published: February 2019

Background:Blattella germanica is a widespread urban invader insect that can spread numerous types of human pathogens, including bacteria, fungi, and protozoa. Despite the medical significance of B. germanica, the genetic diversity of this species has not been investigated across its wide geographical distribution in China.Objective:In this study, the genetic variation of B. germanica was evaluated in central China.Methods:Fragments of the mitochondrial cytochrome c oxidase subunit I (COI) gene and the 16S rRNA gene were amplified in 36 B. germanica isolates from 7 regions. The sequence data for COI and 16S rRNA genes were analyzed using bioinformatics methods.Results:In total, 13 haplotypes were found among the concatenated sequences. Each sampled population, and the total population, had high haplotype diversity (Hd) that was accompanied by low nucleotide diversity (Pi). Molecular genetic variation analysis indicated that 84.33% of the genetic variation derived from intra-region sequences. Phylogenetic analysis indicated that the B. germanica isolates from central China should be classified as a single population. Demographic analysis rejected the hypothesis of sudden population expansion of the B. germanica population.Conclusion:The 36 isolates of B. germanica sampled in this study had high genetic variation and belonged to the same species. They should be classified as a single population. The mismatch distribution analysis and BSP analysis did not support a demographic population expansion of the B. germanica population, which provided useful knowledge for monitoring changes in parasite populations for future control strategies.
Relevance of Machine Learning Techniques and Various Protein Features in Protein Fold Classification: A Review
Authors: Patil Komal , Chouhan Usha 
Published: February 2019

Background:Protein fold prediction is a fundamental step in Structural Bioinformatics. The tertiary structure of a protein determines its function and to predict its tertiary structure, fold prediction serves an important role. Protein fold is simply the arrangement of the secondary structure elements relative to each other in space. A number of studies have been carried out till date by different research groups working worldwide in this field by using the combination of different benchmark datasets, different types of descriptors, features and classification techniques.Objective:In this study, we have tried to put all these contributions together, analyze their study and to compare different techniques used by them.Methods:Different features are derived from protein sequence, its secondary structure, different physicochemical properties of amino acids, domain composition, Position Specific Scoring Matrix, profile and threading techniques.Conclusion:Combination of these different features can improve classification accuracy to a large extent. With the help of this survey, one can know the most suitable feature/attribute set and classification technique for this multi-class protein fold classification problem.
Wing Thickness Optimization for Box Wing Aircraft
Authors: Olugbeji Jemitola P. , Gabriel Okafor E. , Abbe Godwin 
Published: February 2019

Background:
In the interest of improving aircraft performance, studies have highlighted
the benefits of Box wing configurations over conventional cantilever aircraft configuration.
Generally, the greater an aircraft's average thickness to chord ratio (τ), the lower the structural
weight as well as volumetric capacity for fuel. On the other hand, the lower the , the greater
the drag reduction. A review of patents related to the Box-wing aircraft was carried out. While
methodologies for optimizing wing thickness of conventional aircrafts have been studied extensively,
limited research work exist on the methodology for optimizing the wing thickness to
chord ratio of the Box wing aircraft configurations.

Methods:
To address this gap, in this work, a two stage optimization methodology based on gradient
search algorithm and regression analysis was implemented for the optimization of Box
wing aircrafts wing thickness to chord ratio. The first stage involved optimizing the All Up Mass
(AUM), Direct Operating Cost (DOC) and Zero Lift Drag Coefficients (CDO), with respect to the
aft and fore sweep angle for some selected τ values. At the second stage, a suitability function (γ) was optimized with respect to the aft and fore sweep angle for some selected τ values. A
comparative study was further carried out using the proposed methodology on similar size cantilever
wing aircraft.

Results:
From the result, an optimal τ value was reached. Also the τ value for the cantilever aircraft
found based on the proposed methodology was similar to the true τ value of the adopted
aircraft, thereby validating the methodology.

Conclusion:
Based on the optimal τ value reached from this work, the Box wing aircraft are
suitable for thin airfoils.
Potential Natural Dual Agonist PPARα/γ-induced Antidiabetic and Antidyslipidemic Properties of Safrole-Free Nutmeg Seed (Myristica fragrans Houtt) Extract
Authors: Lestari Keri , Diantini Ajeng , Barliana Melisa I. , Achmad Tri H. , Subarnas Anas , Mutakin  , Abdulah Rizky , Lesmana Ronny , Hwang Jae K. 
Published: February 2019

Background:
Nutmeg (Myristica fragrans Houtt) has the potential to control blood glucose
and lipid levels. However, it contains safrole compounds, which are dangerous when humans
consume them.

Objective:
In this study, we eliminated safrole from nutmeg and investigated its antidiabetic and antidyslipidemic
properties.

Methods:
Nutmeg seeds were powdered, extracted with 90% ethanol, and chromatographed to remove
the safrole. Safrole-free nutmeg extract was concentrated, and its antidiabetic and antidyslipidemic
agents were tested in a type 2 diabetes mellitus rat model.

Results:
The results showed that the blood glucose level decreased by 20% after 2 days of treatment,
30% after 4 days of treatment and 40% after 6 days of treatment with nutmeg extracts. The blood
triglyceride level did not change in the first 2 days of treatment, and it decreased by 25% after 4
days of treatment and by another 25% after 6 days of treatment. Luciferase assay showed that safrole-
free nutmeg increased the activities of PPAR α and γ in a dose-dependent manner, which
marked the potential mechanism of lowering the triglyceride and glucose levels.

Conclusion:
Results thus obtained are suggestive of the potential dual effect of safrole-free nutmeg
extract on antidiabetic and antidyslipidemic management.
Low-Dose Lithium for Long-Term Treatment of Clozapine- Induced Neutropenia: A Case Series
Authors: Baig Muhammad R. 
Published: February 2019

Background:
Treatment-resistant psychosis makes schizophrenia a disabling
and costly illness. Clozapine is an effective treatment for treatment-resistant psychosis,
though it is underutilized mainly due to prescribing providers’ fear of a serious yet uncommon
complication, clozapine-induced neutropenia. Clozapine-induced neutropenia
predisposes patients to potentially life-threatening infections leading prescribers to stop use
when blood counts start to drop even well above the recommended cut-off point. Colonystimulating
factors are effective options for reducing risk and duration of neutropeniarelated
events though they add a significant cost burden to the patient and healthcare system.
There is a great need for feasible and cost-effective pharmacotherapies in the mental
health care setting for the management of clozapine-induced neutropenia.

Objective:
We evaluated adjunctive use of lithium when prescribed at a low-dose to stabilize
dropping blood count in patients receiving clozapine for treatment-resistant psychosis.

Methods & Results:
A case series analysis of three patients who were followed in a mental
health outpatient clinic for the management of schizophrenia. Blood counts of all the patients
were stabilized by low-dose lithium treatment and continued to receive long term
treatment of clozapine.

Conclusion:
Results suggest low-dose lithium as a feasible and cost-effective pharmacotherapeutic
option enabling the continuation of clozapine, an effective treatment for
treatment-resistant psychosis.
Cost–effectiveness of elagolix versus leuprolide acetate for treating moderate-to-severe endometriosis pain in the USA
Authors: Wang Si-Tien , Johnson Scott J , Mitchell Dominic , Soliman Ahmed M , Vora Jamie B , Agarwal Sanjay K 
Published: February 2019

Aim:To assess the cost–effectiveness of elagolix versus leuprolide acetate in women with moderate to severe endometriosis pain.Methods:A Markov model was developed. The efficacy of leuprolide acetate was derived from statistical prediction models using elagolix trial data. Model inputs were extracted from Phase III clinical trials and published literature.Results:Compared with leuprolide acetate, elagolix generated positive net monetary benefit (NMB) assuming a payer's willingness-to-pay threshold of US$100,000 per quality-adjusted life year over a 1-year time horizon: US$5660 for elagolix 150 mg and US$6443 for elagolix 200 mg. The 2-year NMBs were also positive.Conclusion:Elagolix was cost effective versus leuprolide acetate in the management of moderate to severe endometriosis pain over 1- and 2-year time horizons. Results were robust in sensitivity analyses.
Los problemas de la métrica de la economía convencional
Authors: Falconí Fander 
Published: February 2019

<p>La medición es una equivocación decisiva que nos está oscureciendo la comprensión de la crisis planetaria. Una distinta economía, articulada con la idea de ciencia post-normal, requiere una métrica distinta a la del reduccio-nismo monetario; es decir, aquella que contempla la incertidumbre y la participación social en los problemas complejos, no como negación del positivismo lógico (que exalta el conocimiento científico y envilece otras formas de conocimiento), sino más bien como una necesidad de ampliar sus dimensiones, en respuesta a la ‘post normalidad’ de la realidad.</p> <p>La métrica no es un problema trivial, sino esencial, por la forma cómo comprendemos el mundo. En un ámbito macro, este artículo cuestiona la métrica tradicional en la economía del “desarrollo” y defiende la necesidad de contar con una nueva métrica multidimensional para comprender y actuar sobre problemas complejos.</p>
AS SANTAS ANAS, OS JOGOS DE PODER E A MEMÓRIA COLETIVA DA BARRIGUDA
Authors: Rossene Andrade Bomfim Tâmara , Silva Costa Edil 
Published: February 2019

Este estudo se baseia nas narrativas da comunidade da Barriguda, município de Ibotirama – BA, sobre a história da Escrava Ana, reconhecida, pela crença popular, como Santa, creditando a sua transformação ao martírio e sofrimento vividos, aos maus tratos do seu Senhor, devido à escrava ter engravidado e dado à luz a
um filho, sem a sua permissão. Testemunhos, depoimentos e relatos de diferentes gerações mantêm viva a memória coletiva sobre a Santa Ana, que também atrai peregrinos de outras regiões. A igreja, ao que parece, procura dissuadir a crença na figura da escrava pobre, negra, subalternizada e marginalizada e, em seu lugar, oferece aos seus devotos a figura consolidada de outra Ana, Santa canonizada, legitimada, com uma aparência
que em nada lembra a figura imaginária da escrava, representativa da Sagrada Família e da hegemonia da Igreja
sobre os seus fiéis. Esse cenário faz ressurgir questionamentos que se relacionam aos sentidos criados pelos habitantes do lugar para as diferentes Anas, sobre a (re)existência da Ana vulnerável, o polo mais fragilizado no jogo de poder que se sustém sobre qual das Anas traz o reflexo da identidade da população local, também
periférica e marginalizada e sobre a luta travada para preservar a imagem da Escrava, existente apenas no imaginário das pessoas, em contraponto à imagem institucionalizada, registrada nos livros sagrados e nas esculturas. A base teórica se constrói a partir das obras de Michel Foucault (2015), Paul Zumthor (2010), Stuart Hall (2008) e Clifford Geertz (2008).
A CARTA COMO PRESENÇA: DA ARTISTA E DA GALERISTA THE LETTER AS EPISTOLARY EXCHANGE: FROM THE ARTIST TO THE GALLERIST
Authors: REBOUÇAS Moema Lúcia Martins , CASTRO Juliana , JANTORNO Alessandra 
Published: February 2019

RESUMO: Este artigo analisa duas cartas escritas na década de 70 do século XX da artista Fayga Ostrower para a Coordenadora da Galeria de Arte e Pesquisa da Universidade Federal do Espírito Santo-GAP/UFES, Jerusa Margarida Gueiros Samu. Utiliza como referencial teórico-metodológico a teoria semiótica desenvolvida por A. J. Greimas e tem como objetivo apreender os sentidos do texto, num percurso que vai do formal ao afetuoso e a tornar explícitos os mecanismos implícitos de estruturação dos textos. As cartas como ato de presença reconstroem a história da artista e de sua relação com a Galeria da UFES. O texto estrutura-se em três partes: na primeira, contextualiza as cartas e discute a prática epistolar; na segunda, analisa as cartas, tendo como metodologia o percurso gerativo de sentido e na terceira, realiza um estudo comparativo entre as duas cartas analisadas. Entendendo a prática epistolar como uma prática dialógica, o trabalho conclui que o simulacro do sujeito inscrito no texto demonstrou a possibilidade da conjunção trabalho-prazer-satisfação. Demonstra, ainda, a partir do percurso do actante-sujeito e dos papéis temáticos aí investidos, a possibilidade de apreensão de novas possibilidades para as relações interpessoais que se dão no ambiente de trabalho e da maneira como deveríamos cultivá-las.Palavras-chave: carta como documento; semiótica; arte ABSTRACT: This article analyzes two letters written in the 70's of the 20th century by the artist Fayga Ostrower for the Coordinator of the Art Gallery of Federal University of Espírito Santo, Jerusa Margarida Gueiros Samu. It uses as theoretical-methodological reference the semiotic theory developed by A. J. Greimas and collaborators. The research aims to apprehend the meanings of the epistolary text, in a path that goes from the formal to the affectionate, making explicit the implicit mechanisms of structuring the texts. The letters as an act of presence reconstruct the history of the artist and her relationship with the UFES Gallery.The text is structured in three parts: in the first, it contextualizes the letters and discusses the epistolary practice; in the second, analyzes the letters having as methodology the generative path of meaning and in the third, compares the two letters. Understanding the epistolary practice as a dialogical practice, the paper concludes that the simulacrum of the subject inscribed in the text demonstrated the possibility of the conjunction work-pleasure-satisfaction-friendship. The research also demonstrates, from the course of the subject actant and the thematic roles invested there, the possibility of apprehension of new possibilities for the interpersonal relationships that occur in the work environment and the way we should cultivate them.Keywords: Letter as document – Semiotics – Art.
UMA ANÁLISE SEMIÓTICA DO DESIGN GRÁFICO RETRÔ: OS ANÚNCIOS DO EVENTO “MAXIMÍDIA 2010” A SEMIOTIC ANALYSIS OF THE RETRO GRAPHIC DESIGN: THE ADS OF THE EVENT “MAXIMÍDIA 2010”
Authors: GOMES Rafael Peduzzi , STEFFEN César , RIBEIRO Vinicius Gadis , CORRÊA Ygor 
Published: February 2019

Resumo: O termo retrô surgiu no século XX, sendo hoje possivelmente banalizado como estilo gráfico, então, este estudo visa a compreender o conceito de retrô enquanto fenômeno no campo do design gráfico. Para tanto, são abordados aspectos formais e simbólicos do design gráfico, assim como investiga-se o retrô etimológica e historicamente. Adota-se a semiótica peirceana como base metodológica para analisar o conjunto de anúncios da campanha publicitária do evento MaxiMídia 2010. A partir da análise, inferiu-se que o retrô não trata apenas dos aspectos sígnicos de ícone e índice, associados a referências do passado, sendo interpretado apenas como símbolo. Nesse sentido, a campanha analisada, ao fazer uma reciclagem de estilos a partir da ironia e da nostalgia reflexiva, contempla algumas definições de retrô propostas pela literatura. Palavras-chave: semiótica, design gráfico, estilo, retrô, campanha publicitária. Abstract: The term retro appeared in the twentieth century, and today is possibly banalized as a graphic style. In this study, it is intended to understand retro as a phenomenon, analyzing how it is presented in graphic design. For that, formal and symbolic aspects of graphic design are approached, and retro is etymologically and historically investigated. Peircean semiotics is used as a methodological basis for analyze the set of ads for the advertising campaign of the event MaxiMídia 2010. According to the analysis, the retro deals not only with the signic aspects of icon and index, with references to the past, but is only interpreted as a symbol. It was considered that the campaign, by doing a recycling of styles from the irony and reflective nostalgia, covers some definitions of retro proposed by the literature. Keywords: semiotics, graphic design, style, retro, advertising campaign.
TRANSUASÃO E COGNOSCIBILIDADE NA POESIA DE DURVALINO COUTO TRANSUASION AND COGNOSCIBILITY IN DURVALINO COUTO’S POETRY
Authors: BEZERRA FILHO Feliciano José , NASCIMENTO Josivan Antônio do 
Published: February 2019

RESUMO: Este artigo, adaptado de capítulo de dissertação, examina como a poesia de Durvalino Couto expressa cognoscibilidade a partir da semiose dos signos verbais no poema. A análise considera o signo in continuum numa relação triádica a fim de elucidar a transuasão que acarreta o universo cognoscível que representa. O estudo leva em conta as contribuições teóricas de Peirce (2010, CP. 1931-1958, 8 vols.), Chandler (2007), Pignatari (2004) e outros. As discussões propostas revelam que a poesia é um arcabouço que se torna cognoscível através da transuasão de conceitos como signos verbais em obsistência com o não-verbal. Essa transuasão constrói um conceito geral para uma representação metafórica daquilo que o poema não é. Por conseguinte, o poema se torna um universo verbal capaz de performar cognição para um possível interpretante. Palavras-chave: Poesia. Signo. Cognoscibilidade. Transuasão. ABSTRACT: This paper, adapted from a dissertation chapter, examines how Durvalino Couto’s poetry can express cognoscibility through the semiosis of verbs signs in a poem. The analysis considers the sign in continuum in a triadic relation in order to elucidate the transuasion that entails the cognizable universe it represents. The study highlights the theoretical framework provided by Peirce (2010, CP, 1931-1958, 8 vols.), Chandler (2007), Pignatari (2004) and others. The intended discussions revealed that poetry is a framework that becomes cognizable through the transuasion of concepts acting as verb signs in obsistence with the non-verbal. This transuasion creates a general concept standing for a metaphorical representation of what the poem is not. As a result, the poem becomes a verbal universe able to perform cognition for a possible interpretant. Keywords: Poetry. Sign. Cognoscibility. Transuasion.
ARCHEOLOGIE ET INTERPRETATION: POUR UNE SEMIOTIQUE DES OBJETS CULTURELS ARCHEOLOGY AND INTERPRETATION: FOR A SEMIOTIC OF CULTURAL OBJECTS
Authors: LYONNET Bernard 
Published: February 2019

Résumé: Dans le cadre du projet de sémiotique des cultures, le sémanticien François Rastier a précisé la notion d’objet culturel et proposé d’étendre les méthodes de la sémantique interprétative à des images et autres signes non-textuel. En parallèle, la nécessité de comprendre le sens d’objets symboliques révélés par l’archéologie est souvent exprimée et problématisée par les protohistoriens. Le croisement de ces deux domaines de recherche permet de poser les contours épistémologiques et méthodologiques d’une interprétation de ce type d’objets et d’en fournir une première illustration.Mots clés: sémiotique des cultures, objet culturel, signes graphiques, interprétation, archéologie, protohistoire Abstract: In the context of the semiotic of cultures project, the semanticist François Rastier has stated the notion of cultural object and has suggested to extend methods of interpretative semantics to images and other non-textual signs. Meanwhile, the necessity to understand the meaning of symbolic objects revealed by the archaeology is often expressed and problematized by protohistorians. The common ground of these two research areas allows us to set the epistemological and methodological outlines of an interpretation of such objects and to provide a first illustration. Keywords: semiotic of cultures, cultural object, graphics signs, interpretation, archaeology, protohistory