bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome monitoring visualizes growth stage‐dependent nutrient status dynamics in rice under field conditions
Authors: Takehisa Hinako , Sato Yutaka 
Published: November 2018

SummaryCrop plants undergo morpho‐physiological changes throughout the growth process in response to both the internal and the external environment, and that eventually determine the yield. The system‐level adjustment of the morpho‐physiological changes has remained largely unclear, however, especially in field conditions. Here, we reveal changes in nutrient status associated with tiller development and soil conditions based on the leaf transcriptome profile of rice (Oryza sativa) throughout the entire period of growth. We performed gene co‐expression network analysis and identified three gene sets as indicators for monitoring the internal nitrogen and phosphorus status. Expression profiling reveals that the phosphorus starvation response is expressed during the tillering stage and is then switched off with the transition to nitrogen deficiency. Coincident with phosphorus status dynamics, the level of phosphate in the leaf is demonstrated to be low during the tillering stage and subsequently increases drastically. The phosphorus dynamics are genetically validated by analysing mutants with a defect in phosphorus homeostasis. Notably, we show that nitrogen limitation directly suppresses the phosphorus starvation response. Finally, the phosphorus starvation response is demonstrated to be activated in soil with a high phosphate retention capacity, without the visible phenotypes associated with phosphorus starvation. Our results reveal a growth stage‐ and soil condition‐dependent reaction that requires phosphorus, which is expressed to promote the phosphorus uptake required for developing tillers and is directly adjusted by nitrogen status. A molecular framework for elucidating nutrient status dynamics under field conditions would provide insights into improving crop productivity.
Diagnosis of diaphragm injuries using modern 256-slice CT scanners: too early to abandon operative exploration
Authors: Uhlich Rindi , Kerby Jeffrey David , Bosarge Patrick , Hu Parker 
Published: November 2018

BackgroundMissed injury of the diaphragm may result in hernia formation, enteric strangulation, and death. Compounding the problem, diaphragmatic injuries are rare and difficult to diagnose with standard imaging. As such, for patients with high suspicion of injury, operative exploration remains the gold standard for diagnosis. As no current data currently exist, we sought to perform a pragmatic evaluation of the diagnostic ability of 256-slice multidetector CT scanners for diagnosing diaphragmatic injuries after trauma.MethodsA retrospective review of trauma patients from 2011 to 2018 was performed at an American College of Surgeons-verified level 1 trauma center to identify the diagnostic accuracy of CT scan for acute diaphragm injury. All patients undergoing abdominal operation were eligible for inclusion. Two separate levels of CT scan technology, 64-slice and 256-slice, were used during this time period. The prospective imaging reports were reviewed for the diagnosis of diaphragm injury and the results confirmed with the operative record. Injuries were graded using operative description per the American Association for the Surgery of Trauma guidelines.ResultsOne thousand and sixty-eight patients underwent operation after preoperative CT scan. Acute diaphragm injury was identified intraoperatively in 14.7%. Most with diaphragmatic injury underwent 64-slice CT (134 of 157, 85.4%). Comparing patients receiving 64-slice or 256-slice CT scan, there was no difference in the side of injury (left side 57.5% vs. 69.6%, p=0.43) or median injury grade (3 (3, 3) vs. 3 (2, 3), p=0.65). Overall sensitivity, specificity, and diagnostic accuracy of the 256-slice CT were similar to the 64-slice CT (56.5% vs. 45.5%, 93.7% vs. 98.1%, and 89.0% vs. 90.2%).DiscussionThe new 256-slice multidetector CT scanner fails to sufficiently improve diagnostic accuracy over the previous technology. Patients with suspicion of diaphragm injury should undergo operative intervention.Level of evidenceI, diagnostic test or criteria.
STING Promotes Homeostasis via Regulation of Cell Proliferation and Chromosomal Stability
Authors: Ranoa Diana Rose E. , Widau Ryan C. , Mallon Stephen , Parekh Akash D. , Nicolae Claudia M. , Huang Xiaona , Bolt Michael J. , Arina Ainhoa , Parry Renate , Kron Stephen J. , Moldovan George-Lucian , Khodarev Nikolai N. , Weichselbaum Ralph R. 
Published: November 2018

Abstract

Given the integral role of stimulator of interferon genes (STING, TMEM173) in the innate immune response, its loss or impairment in cancer is thought to primarily affect antitumor immunity. Here we demonstrate a role for STING in the maintenance of cellular homeostasis through regulation of the cell cycle. Depletion of STING in human and murine cancer cells and tumors resulted in increased proliferation compared with wild-type controls. Microarray analysis revealed genes involved in cell-cycle regulation are differentially expressed in STINGko compared with WT MEFs. STING-mediated regulation of the cell cycle converged on NFκB- and p53-driven activation of p21. The absence of STING led to premature activation of cyclin-dependent kinase 1 (CDK1), early onset to S-phase and mitosis, and increased chromosome instability, which was enhanced by ionizing radiation. These results suggest a pivotal role for STING in maintaining cellular homeostasis and response to genotoxic stress.

Significance:
These findings provide clear mechanistic understanding of the role of STING in cell-cycle regulation, which may be exploited in cancer therapy because most normal cells express STING, while many tumor cells do not.
See related commentary by Gius and Zhu, p. 1295
Aspirin, NSAIDs, and Glioma Risk: Original Data from the Glioma International Case–Control Study and a Meta-analysis
Authors: Amirian E. Susan , Ostrom Quinn T. , Armstrong Georgina N. , Lai Rose K. , Gu Xiangjun , Jacobs Daniel I. , Jalali Ali , Claus Elizabeth B. , Barnholtz-Sloan Jill S. , Il'yasova Dora , Schildkraut Joellen M. , Ali-Osman Francis , Sadetzki Siegal , Jenkins Robert B. , Lachance Daniel H. , Olson Sara H. , Bernstein Jonine L. , Merrell Ryan T. , Wrensch Margaret R. , Johansen Christoffer , Houlston Richard S. , Scheurer Michael E. , Shete Sanjay , Amos Christopher I. , Melin Beatrice , Bondy Melissa L. 
Published: November 2018

Abstract

Background:
There have been few studies of sufficient size to address the relationship between glioma risk and the use of aspirin or NSAIDs, and results have been conflicting. The purpose of this study was to examine the associations between glioma and aspirin/NSAID use, and to aggregate these findings with prior published studies using meta-analysis.

Methods:
The Glioma International Case-Control Study (GICC) consists of 4,533 glioma cases and 4,171 controls recruited from 2010 to 2013. Interviews were conducted using a standardized questionnaire to obtain information on aspirin/NSAID use. We examined history of regular use for ≥6 months and duration-response. Restricted maximum likelihood meta-regression models were used to aggregate site-specific estimates, and to combine GICC estimates with previously published studies.

Results:
A history of daily aspirin use for ≥6 months was associated with a 38% lower glioma risk, compared with not having a history of daily use [adjusted meta-OR = 0.62; 95% confidence interval (CI), 0.54–0.70]. There was a significant duration-response trend (P = 1.67 × 10−17), with lower ORs for increasing duration of aspirin use. Duration-response trends were not observed for NSAID use. In the meta-analysis aggregating GICC data with five previous studies, there was a marginally significant association between use of aspirin and glioma (mOR = 0.84; 95% CI, 0.70–1.02), but no association for NSAID use.

Conclusions:
Our study suggests that aspirin may be associated with a reduced risk of glioma.

Impact:
These results imply that aspirin use may be associated with decreased glioma risk. Further research examining the association between aspirin use and glioma risk is warranted.
Leveraging TCR Affinity in Adoptive Immunotherapy against Shared Tumor/Self-Antigens
Authors: Miller Aaron M. , Bahmanof Milad , Zehn Dietmar , Cohen Ezra E.W. , Schoenberger Stephen P. 
Published: November 2018

AbstractAdoptive cellular therapy (ACT) using T-cell receptor (TCR)–engineered lymphocytes holds promise for eradication of disseminated tumors but also an inherent risk of pathologic autoimmunity if targeted antigens or antigenic mimics are expressed by normal tissues. We evaluated whether modulating TCR affinity could allow CD8+ T cells to control tumor outgrowth without inducing concomitant autoimmunity in a preclinical murine model of ACT. RIP-mOVA mice express a membrane-bound form of chicken ovalbumin (mOVA) as a self-antigen in kidney and pancreas. Such mice were implanted with OVA-expressing ID8 ovarian carcinoma cells and subsequently treated with CD8+ T lymphocytes (CTL) expressing either a high-affinity (OT-I) or low-affinity (OT-3) OVA-specific TCR. The effects on tumor growth versus organ-specific autoimmunity were subsequently monitored. High-affinity OT-I CTLs underwent activation and proliferation in both tumor-draining and pancreatic lymph nodes, leading to both rapid eradication of ID8-OVA tumors and autoimmune diabetes in all treated mice. Remarkably, the low-affinity OT-3 T cells were activated only by tumor-derived antigen and mediated transient regression of ID8-OVA tumors without concomitant autoimmunity. The OT-3 cells eventually upregulated inhibitory receptors PD-1, TIM-3, and LAG-3 and became functionally unresponsive, however, allowing the tumors in treated mice to reestablish progressive growth. Antibody-mediated blockade of the inhibitory receptors prevented exhaustion and allowed tumor clearance, but these mice also developed autoimmune diabetes. The findings reveal that low-affinity TCRs can mediate tumor regression and that functional avidity can discriminate between tumor-derived and endogenous antigen, while highlighting the risks involved in immune-checkpoint blockade on endogenous self-reactive T cells.
A first record of the Redbelly Yellowtail Fusilier Caesio cuning (Bloch, 1791) (Teleostei: Caesionidae) from Visakhapatnam coastal waters, India
Authors: Naranji Muddula Krishna , Velamala Govinda Rao , Babu Kondamudi Ramesh 
Published: November 2018

The Redbelly Yellowtail Fusilier Caesio cuning (Bloch, 1791) is recorded for the first time from the coastal waters of Visakhapatnam, India. This paper provides detailed description, morphometric and meristic characters.
Odonata of eastern Bangladesh with three new records for the country
Authors: Khan M. Kawsar 
Published: November 2018

A study was conducted in the eastern region of Bangladesh to contribute to the knowledge of the country’s Odonata fauna. A total of 75 species belonging to nine families was recorded during the study period from April 2014 to July 2016. Two zygopteran species, Calicnemia imitans and Prodasineura autumnalis, and one anisopteran species, Megalogomphus smithii, are new records for the country. The Megalogomphus genus is recorded for the first time from Bangladesh.
Breeding reports and conservation implications of the Endangered Black-bellied Tern Sterna acuticauda J.E. Gray, 1831 (Aves: Charadriiformes: Laridae) in Odisha, eastern India
Authors: Kar Tuhinansu , Palei Himanshu Shekhar , Debata Subrat 
Published: November 2018

The Black-bellied Tern is an endangered species and its population is declining severely due to the loss and degradation of its foraging and breeding habitats because of increasing anthropogenic activities. We report the breeding of Black-bellied Tern from different localities along the Mahanadi River in Odisha, eastern India. We recommend the protection and conservation of its breeding sites along with regular community outreach activities for the long-term conservation of this globally threatened species.
A new record of the Malay Cardamom Amomum aculeatum Roxb. (Zingiberaceae) for mainland India
Authors: Patil Sameer Chandrakant , Lakshminarasimhan P. 
Published: November 2018

Amomum aculeatum Roxb. (Zingiberaceae), earlier known from the Andaman & Nicobar Islands, is reported from Kodagu in Karnataka, for the first time from the mainland of India. A detailed description of the species with relevant information and distribution are provided.
First record of colour aberration in Basra Reed Warbler Acrocephalus griseldis (Hartlaub, 1891) (Passeriformes: Acrocephalidae) from Central Marshes of southern Iraq, with notes on its intraspecific/interspecific behavior
Authors: Al-Sheikhly Omar F. , Haba Mukhtar K. , Faza’a Nadheer A. , Al-Asady Ra’ad H. 
Published: November 2018

Pigment disorders such as albinism, leucism and progressive greying, which cause the absence of melanin pigments in all or parts of the plumage and bare parts, have been reported in many wild bird populations including Acrocephalus warblers. Basra Reed Warbler Acrocephalus griseldis (Hartlaub, 1891) is a restricted-range species confined to the extensive reed beds of Mesopotamian marshes. It is listed as Endangered due to breeding habitat degradation, water scarcity and climate change. In April 2018, a partly white plumaged Basra Reed Warbler was sighted in Central Marshes in southern Iraq. This is the first report of such a plumage aberration in this species. The nature of the aberration involved an intraspecific/interspecific behavior of the white plumaged Basra Reed Warbler are described.
The RheaG Weather Generator Algorithm: Evaluation in Four Contrasting Climates from the Iberian Peninsula
Authors: Nadal-Sala Daniel , Gracia Carlos A. , Sabaté Santiago 
Published: November 2018

AbstractThis paper describes the assumptions, equations, and procedures of the RheaG weather generator algorithm (WGA). RheaG was conceived for the generation of robust daily meteorological time series, whether in static or transient climate conditions. Here we analyze its performance in four Iberian locations—Bilbao, Barcelona, Madrid, and Sevilla—with differentiated climate characteristics. To validate the RheaG WGA, we compared observed and generated meteorological time series’ statistical properties of precipitation, maximum temperature, and minimum temperature for all four locations. We also compared observed and simulated rain events spell length probabilities in all four locations. Finally, RheaG includes two weather generation procedures: one in which monthly mean values for meteorological variables are unconstrained and one in which they are constrained according to a predefined baseline climate variability. Here, we compare the two weather generation procedures included in RheaG using the observed data from Barcelona. Our results present a high agreement in the statistical properties and the rain spell length probabilities between observed and generated meteorological time series. Our results show that RheaG accurately reproduces seasonal patterns of the observed meteorological time series for all four locations, and it is even able to differentiate two climatic seasons in Bilbao that are also present in the observed data. We find a trade-off between generation procedures in which the unconstrained procedure better reproduces the variability of monthly and yearly precipitation than the constrained one, but the constrained procedure is able to keep the same climatic signal across meteorological time series. Thus, the first procedure is more accurate, but the latter is able to maintain spatial autocorrelation among generated meteorological time series.
Reporting of Cosmesis in Head and Neck Reconstruction: A Systematic Review
Authors: Vila Peter M. , Ramsey Tam , Yaeger Lauren H. , Desai Shaun C. , Branham Gregory H. 
Published: November 2018

ObjectiveTo identify the method and rate at which cosmesis is reported after reconstruction from head and neck surgery among adults.Data SourcesA medical librarian implemented search strategies in multiple databases for head and neck reconstruction, outcome assessment/patient satisfaction, and cosmesis/appearance.Review MethodsInclusion and exclusion criteria were designed to capture studies examining adults undergoing reconstruction after head and neck cancer surgery with assessment of postoperative cosmesis. The primary outcome was the method to assess cosmesis. Secondary outcomes were types of instruments used and the rate at which results were reported. Validated instruments used in these studies were compared and critically assessed.ResultsThe search identified 4405 abstracts, and 239 studies met inclusion and exclusion criteria. Of these, 43% (n = 103) used a scale or questionnaire to quantify the cosmetic outcome: 28% (n = 66), a visual analog, Likert, or other scale; 13% (n = 30), a patient questionnaire; and 3% (n = 7), both. Of the 103 studies that used an instrument, 14% (n = 14, 6% overall) used a validated instrument. The most common validated instrument was the University of Washington Quality of Life (UWQOL) questionnaire (4%, n = 9). The most highly rated instruments were the UWQOL and the Derriford Appearance Scale.ConclusionsReporting of cosmetic outcomes after head and neck cancer reconstruction is heterogeneous. Most studies did not report patient feedback, and a minority used a validated instrument to quantify outcomes. To reduce bias, improve reliability, and decrease heterogeneity, we recommend the UWQOL to study cosmetic outcomes after head and neck reconstruction.
Female Soccer Players With Anterior Cruciate Ligament Reconstruction Have a Higher Risk of New Knee Injuries and Quit Soccer to a Higher Degree Than Knee-Healthy Controls
Authors: Fältström Anne , Kvist Joanna , Gauffin Håkan , Hägglund Martin 
Published: November 2018

Background: Many patients with anterior cruciate ligament (ACL) reconstruction who return to sport suffer new ACL injuries or quit sports soon after returning. Purpose: To prospectively follow a cohort of female soccer players with primary unilateral ACL reconstruction and matched knee-healthy controls from the same soccer teams to compare (1) the rate of new traumatic and nontraumatic knee injuries and other injuries, (2) the proportion of players who quit soccer, and (3) player-reported activity level and satisfaction with activity level and knee function. Study Design: Cohort study; Level of evidence, 2. Methods: A total of 117 active female soccer players (mean ± SD age, 19.9 ± 2.5 years) 18.9 ± 8.7 months after ACL reconstruction and 119 knee-healthy female soccer players (19.5 ± 2.5 years) matched from the same teams were prospectively followed for 2 years for new knee injuries, other injuries, soccer playing level, activity level according to the Tegner Activity Scale, and satisfaction with activity level and knee function. Results: Players with ACL reconstruction had a higher rate of new ACL injuries (n = 29 vs 8; 19 vs 4 per 100 player years; rate ratio [RR], 4.82; 95% CI, 2.20-10.54; P < .001), other traumatic knee injuries (29 vs 16 per 100 player years; RR, 1.84; 95% CI, 1.16-2.93; P < .01), and nontraumatic knee injuries (33 vs 9 per 100 player years; RR, 3.62; 95% CI, 2.11-6.21; P < .001) as compared with controls. There was no difference in the rate of other (not knee) injuries (43 vs 48 per 100 player years; RR, 0.90; 95% CI, 0.65-1.23; P = .494). During the 2-year follow-up, 72 (62%) players with ACL reconstruction quit soccer, as opposed to 43 (36%) controls ( P = .001). The median Tegner Activity Scale score decreased in both groups ( P < .001) but more for the ACL-reconstructed group ( P < .015). Conclusion: Female soccer players with ACL reconstruction had nearly a 5-fold-higher rate of new ACL injuries and a 2- to 4-fold-higher rate of other new knee injuries, quit soccer to a higher degree, and reduced their activity level to a greater extent as compared with knee-healthy controls.
Predictors of 5-Year Citation Rate in the Orthopaedic Sports Medicine Literature
Authors: Movassagi Kamran , Kunze Kyle N. , Beck Edward C. , Fu Michael C. , Nho Shane J. 
Published: November 2018

Background: The citation rate of a research published article is an indicator of its quality and impact and contributes to the journal’s impact factor. Within the orthopaedic sports medicine literature, predictors of citation rates have not been previously described. Purpose: To identify characteristics of published articles that predict 5-year citation rates of studies in the orthopaedic sports medicine literature. Study Design: Cross-sectional study. Methods: Research articles published in The American Journal of Sports Medicine (AJSM), Arthroscopy: The Journal of Arthroscopic and Related Surgery, and Knee Surgery, Sports Traumatology, Arthroscopy (KSSTA) from 2012 were analyzed. Extracted characteristics of published articles included journal, author number, origin of study, first author degree, subject of study, study type, sample size, number of references and institutions, conflicts of interest, level of evidence, and 5-year citation rates. Multivariate logistic regression was used to determine predictors of greater than the mean number of citations at 5 years. Results: A total of 825 published articles ( AJSM, n = 313; Arthroscopy, n = 173; KSSTA, n = 339) were included in the final analysis. The mean number of 5-year citations was 23.2 (95% CI, 21.6-24.9; range, 1.0-260.0). AJSM had a significantly greater citation rate (32.4) than Arthroscopy (21.7) and KSSTA (15.2) ( P < .001 for both). Arthroscopy had a greater citation rate than KSSTA ( P = .008). Independent predictors of greater than the mean number of citations at 5 years were published articles in AJSM (odds ratio [OR], 5.17; 95% CI, 2.81-9.52; P < .0001), published articles of North American origin (OR, 1.79; 95% CI, 1.25-2.58; P = .002), and published articles regarding the hip (OR, 2.68; 95% CI, 1.08-6.67; P = .035). Conclusion: Published articles in AJSM, those from North America, and those examining the hip were independent predictors of greater citation rates at 5 years.