bluuebunny/crossref_metadata_2025_split huggingface dataset:
Periodontal healing by periodontal ligament fiber with or without cells: A preclinical study of the decellularized periodontal ligament in a tooth replantation model
Authors: Lee Jung‐Seok , Kim Seul‐Ki , Gruber Reinhard , Kim Chang‐Sung 
Published: July 2019

AbstractBackgroundThe periodontal ligament (PDL) comprises cells embedded in the extracellular matrix (ECM) and is required for periodontal healing upon tooth transplantation. However, the extent to which the ECM contributes to periodontal regeneration remains unknown. This study aimed to evaluate effects of separate PDL matrix without cells on periodontal healing.MethodsMandibular premolars extracted from six beagle dogs, were 1) left untreated, 2) decellularized, or 3) surface planed. Cytologic, histologic, scanning electron microscopy, and transmission electron microscopy analyses confirmed that detergents could be used to decellularize the PDL. An additional circumferential osteotomy was performed to replant dental roots into extraction sockets. Radiography and histology were used to evaluate periodontal regeneration 8 weeks later, with the data adjusted for multiple testing.ResultsIn pristine extraction sites, total root resorption (P = 0.034), recovered PDL space (P = 0.012), and new cementum (P = 0.004) were greater in untreated teeth than in roots that underwent surface planing. There were no significant changes when comparing untreated teeth with teeth having a decellularized PDL (P = 0.081, P = 0.170, and P = 1.000, respectively), and decellularized teeth showed significant increase of new cementum compared to surface planed teeth (P = 0.048). In the defect area, only the recovered PDL space (P = 0.034) was greater in untreated teeth when comparing with denuded roots.ConclusionThese results suggest that in addition to untreated PDL, decellularized PDL also partially supports reattachment (particularly cementogenesis) in pristine extraction sites but not in defect areas.
Siliceous‐based monolithic materials coated with a hydroxyapatite layer: Preparation and investigation of drug affinity by Raman spectroscopy
Authors: Zielińska Monika , Buchwald Tomasz , Marańda Monika , Voelkel Adam 
Published: July 2019

AbstractMonolithic materials based on silica and hydroxyapatite was applied as tool for investigation of interactions between bone and potential antiresorptive drugs. First type of material was created on the basis of tetramethyl orthosilicate and trimethoxymethylsilane. Second type of material contained additionally chitosan in its structure. Ceramic fraction of monoliths was prepared in two ways: by incorporation of hydroxyapatite during crosslinking or by using soaking method (hydroxyapatite mineralization on the surface). Monolithic materials were prepared in two different forms: in the form of a cylinder and as a filling of a needle for monolithic in‐needle extraction (MINE) device. Several techniques were applied to evaluate the properties of 19 prepared materials: energy dispersive X‐ray spectroscopy, scanning electron microscopy, and Raman spectroscopy, which allowed to confirm and characterize occurred hydroxyapatite layer. Sorption–desorption investigation, with the sodium risedronate as test compound, was made with using ultraviolet‐visible spectroscopy, to assess the affinity of the bisphosphonate for the ceramic layer of the material as a bone substitute. One of 15 prepared MINE devices exhibits sorption ability of risedronate equal to 97%.
Lighting intensity and photoperiod serves tailoring nitrate assimilation indices in red and green baby leaf lettuce
Authors: Viršilė Akvilė , Brazaitytė Aušra , Vaštakaitė‐Kairienė Viktorija , Miliauskienė Jurga , Jankauskienė Julė , Novičkovas Algirdas , Samuolienė Giedrė 
Published: July 2019

AbstractBACKGROUNDUnderstanding plant responses to light quantity in indoor horticultural systems is important for optimising lettuce growth and metabolism as well as energy utilisation efficiency. Light intensity and photoperiod sufficient for normal plant growth parameters might be not efficient for nitrate assimilation. Therefore, this study explored and compared the effects of different light intensities (100–500 μmol m−2 s−1) and photoperiods (12–24 h) on the growth and nitrate assimilation in red and green leaf lettuce (Lactuca sativa L.).RESULTSFor efficient nitrate assimilation, 300–400 μmol m−2 s−1 photosynthetic photon flux density (PPFD) and 16–18 h photoperiod is necessary for red and green lettuces. The insufficient light quantity resulted in reduced growth and remarkable increase in nitrate and nitrite contents in both cultivars. Short photoperiods, similarly to low PPFD, growth parameters, chlorophyll indices and nitrate assimilation indices showed the shortage of photosynthetic products for normal plant physiological processes. Short photoperiods had the least pronounced effect on nitrate and nitrite contents in lettuce leaves.CONCLUSIONLight intensity was superior compared to photoperiods for efficient nitrate assimilation in both lettuce cultivars. Under short photoperiods, similarly to low intensity, growth parameters, chlorophyll index and nitrate assimilation indices showed a shortage of photosynthetic products for normal physiological processes. The free amino acid concentration increased, but it was not efficiently incorporated in proteins, as their level in lettuce was lower compared to those for moderate photoperiods. © 2019 Society of Chemical Industry
Developing a miniaturized setup for in‐tube simultaneous determination of three alkaloids using electromembrane extraction in combination with ultraviolet spectrophotometry
Authors: Pourahadi Ahmad , Farahani Ali , Hosseiny Davarani Saied Saeed , Nojavan Saeed , Tashakori Camellia 
Published: July 2019

AbstractHerein, electromembrane extraction was combined with ultraviolet spectrophotometry using a customized manifold for preconcentration and simultaneous determination of morphine, codeine, and papaverine in water and human urine samples. Absorption spectra of the extracts were recorded inside the lumen of the hollow fiber using two fiber optics connected to a miniature spectrophotometer. Partial least squares regression was applied to resolve the overlapped spectra of the analytes. Performance of the model was validated by an independent test set. Central composite design was applied to optimize the extraction parameters. The optimized extraction conditions are as follows; supporting liquid membrane: 2‐nitrophenyl octyl ether containing 15% v/v bis(2‐ethylhexyl) phosphate, applied voltage: 80 V, donor pH: 3.0, acceptor pH: 1.0, extraction time: 20 min. Finally, the optimized extraction method was validated for determination of the mentioned alkaloids in human urine samples. The method showed good linearity (R2 > 0.995) for all of the mentioned alkaloids. The limits of detection for morphine, codeine, and papaverine in diluted human urine were found to be 0.6, 1.1, and 0.6 ng/mL, respectively with acceptable relative standard deviations. Enrichment factors of 104, 108, and 102 were achieved for morphine, codeine, and papaverine, respectively.
Pelagic methane oxidation in the northern Chukchi Sea
Authors: Rogener Mary Katherine , Sipler Rachel E. , Hunter Kimberley S. , Bronk Deborah A. , Joye Samantha B. 
Published: July 2019

AbstractSignificant amounts of methane reside in sediments along the continental margins and slope of the Arctic Ocean. Methanotrophic bacteria oxidize methane to bicarbonate and also assimilate some methane‐derived carbon into biomass. Their metabolism transforms methane to other forms of carbon and sequesters it within the system, reducing its emission to the atmosphere. Increases in water temperatures driven by global climate change may accelerate the methane flux from the benthos into the water column, potentially increasing the importance of methanotrophic consumption as a methane sink. We report methane concentrations and oxidation rates in the water column of the Chukchi Sea from August 2017. This area is characterized by seasonally high nutrient concentrations that fuel high rates of pelagic primary productivity and subsequent sedimentation of organic matter, which stimulates benthic methanogenesis. Methane concentrations in the study area ranged from 6 to 72 nmol L−1, consistent with previously published measurements. Methane oxidation rates were as high as 580 pmol L−1 d−1, similar to the rates measured in the East Siberian Arctic Shelf. Depth‐integrated methane oxidation rates were lower than methane efflux rates, suggesting that physiochemical factors prevent the methanotrophic microbial community from efficiently removing methane from the ecosystem. Still, methanotrophic bacteria provide an ecosystem service by removing a fraction of methane prior to its efflux to the atmosphere.
Light exposure along particle flowpaths in large rivers
Authors: Gardner John R. , Ensign Scott H. , Houser Jeffrey N. , Doyle Martin W. 
Published: July 2019

AbstractSunlight is a critical resource in aquatic systems driving photosynthesis, photodegradation of organic matter and contaminants, animal behavior, and the activity of human pathogens. In rivers, solutes, materials, and organisms are turbulently mixed across the water column during downstream transport and exposed to highly variable sunlight. However, there are no measurements of suspended particles' sunlight exposure during downstream transport to characterize this variability, and it is unclear if current measurement approaches and optical theory capture the light exposure of suspended particles. We deployed neutrally buoyant drifters and stationary buoys in the Upper Mississippi (WI, U.S.A.) and Neuse Rivers (NC, U.S.A.) to measure underwater sunlight from the perspective of suspended particles. In our study sites, underwater sunlight varied more along flowpaths measured by drifters than over time measured by fixed‐site buoys; sunlight exposure along flowpaths was dominated by bursts of light (sunflecks) that accounted for 62–99% of the cumulative sunlight exposure; and modeled sunlight exposure using optical theory was consistently 56–1700% higher than measured sunlight exposure along flowpaths. Our results suggested that suspended particles in the study reaches experienced darker conditions than predicted and have important implications for how to quantify underwater sunlight in rivers.
Searching for the one: Customer relationship management software selection
Authors: Cricelli Livio , Famulari Federico Maria , Greco Marco , Grimaldi Michele 
Published: July 2019

AbstractIn a competitive environment that increasingly awards a clever approach to customer relationship management (CRM), firms need to systematize the way they interact with their customers. The relationships that often lay in the hands of managers and salespeople need to be thoughtfully organized to maximize both customer satisfaction and the effectiveness of the marketing efforts. CRM software packages can be an answer to organize and systematize the management of such commercial relationships. However, decision makers may not have the time and the competencies to identify the most suitable solution for their needs, among the hundreds existing, and may ultimately resort to an external expert. Since the existing methods to select a CRM software package suffer from several limitations, this article introduces a novel four‐step method allowing to actively involve the decision makers in the CRM software package selection, simultaneously minimizing the effort requested to them and maximizing the extent to which the final choice suits their specific needs and preferences. The method resorts to a coordinated use of the analytic hierarchy process and of its fuzzy adaptation. The article also presents an exemplification of the method in a small Italian firm.
Cancer outcomes among Parkinson's disease patients with leucine rich repeat kinase 2 mutations, idiopathic Parkinson's disease patients, and nonaffected controls
Authors: Agalliu Ilir , Ortega Roberto A. , Luciano Marta San , Mirelman Anat , Pont‐Sunyer Claustre , Brockmann Kathrin , Vilas Dolores , Tolosa Eduardo , Berg Daniela , Warø Bjørg , Glickman Amanda , Raymond Deborah , Inzelberg Rivka , Ruiz‐Martinez Javier , Mondragon Elisabet , Friedman Eitan , Hassin‐Baer Sharon , Alcalay Roy N. , Mejia‐Santana Helen , Aasly Jan , Foroud Tatiana , Marder Karen , Giladi Nir , Bressman Susan , Saunders‐Pullman Rachel 
Published: July 2019

ABSTRACTBackgroundIncreased cancer risk has been reported in Parkinson's disease (PD) patients carrying the leucine rich repeat kinase 2 (LRRK2) G2019S mutation (LRRK2‐PD) in comparison with idiopathic PD (IPD). It is unclear whether the elevated risk would be maintained when compared with unaffected controls.MethodsCancer outcomes were compared among 257 LRRK2‐PD patients, 712 IPD patients, and 218 controls recruited from 7 LRRK2 consortium centers using mixed‐effects logistic regression. Data were then pooled with a previous study to examine cancer risk between 401 LRRK2‐PD and 1946 IPD patients.ResultsAlthough cancer prevalence was similar among LRRK2‐PD patients (32.3%), IPD patients (27.5%), and controls (27.5%; P = 0.33), LRRK2‐PD had increased risks of leukemia (odds ratio [OR] = 4.55; 95% confidence interval [CI], 1.46–10.61) and skin cancer (OR = 1.61; 95% CI, 1.09–2.37). In the pooled analysis, LRRK2‐PD patients had also elevated risks of leukemia (OR = 9.84; 95% CI, 2.15–44.94) and colon cancer (OR = 2.34; 95% CI, 1.15–4.74) when compared with IPD patients.ConclusionsThe increased risks of leukemia as well as skin and colon cancers among LRRK2‐PD patients suggest that LRRK2 mutations heighten risks of certain cancers. © 2019 International Parkinson and Movement Disorder Society
Miniaturized low‐pass filter design with wide stopband using complementary split‐ring resonator
Authors: Ramanuajam Parthasarathy , Ramesh Venkatesan P. G. , Arumugam Chandrasekar 
Published: July 2019

AbstractA miniaturized microstrip low‐pass filter (LPF) design comprises of meander lines, rectangular patches, open‐circuit stubs, and a complementary split‐ring resonator placed in between the input/output ports has been presented in this article. The proposed filter has insertion loss of 0.10 dB in passband with a bandwidth of 3.8 GHz. It also has a wide stopband bandwidth of 9.9 GHz with minimum attenuation of 28 dB (between 4.1 GHz and 14 GHz). Wide stopband has been achieved by introducing the open stubs and impedance transforms the patch resonators. Group delay of designed LPF quantified to be less than 0.22 ns in the passband. The simulated and measured results have been compared and found to be in good agreement. This filter finds its applications in Global System for Mobile Communications (GSM) and the industrial, scientific and medical (ISM) bands with harmonic suppression in modern communication receivers.
Accuracy of Resting Energy Expenditure Predictive Equations in Patients With Cancer
Authors: Purcell Sarah A. , Elliott Sarah A. , Baracos Vickie E. , Chu Quincy S. C. , Sawyer Michael B. , Mourtzakis Marina , Easaw Jacob C. , Spratlin Jennifer L. , Siervo Mario , Prado Carla M. 
Published: July 2019

AbstractBackgroundOur purpose was to assess the accuracy of resting energy expenditure (REE) equations in patients with newly diagnosed stage I–IV non–small cell lung, rectal, colon, renal, or pancreatic cancer.MethodsIn this cross‐sectional study, REE was measured using indirect calorimetry and compared with 23 equations. Agreement between measured and predicted REE was assessed via paired t‐tests, Bland‐Altman analysis, and percent of estimations ≤ 10% of measured values. Accuracy was measured among subgroups of body mass index (BMI), stage (I–III vs IV), and cancer type (lung, rectal, and colon) categories. Fat mass (FM) and fat‐free mass (FFM) were assessed using dual x‐ray absorptiometry.ResultsAmong 125 patients, most had lung, colon, or rectal cancer (92%, BMI: 27.5 ± 5.6 kg/m2, age: 61 ± 11 years, REE: 1629 ± 321 kcal/d). Thirteen (56.5%) equations yielded REE values different than measured (P < 0.05). Limits of agreement were wide for all equations, with Mifflin–St. Jeor equation having the smallest limits of agreement, −21.7% to 11.3% (−394 to 203 kcal/d). Equations with FFM were not more accurate except for one equation (Huang with body composition; bias, limits of agreement: −0.3 ± 11.3% vs without body composition: 2.3 ± 10.1%, P < 0.001). Bias in body composition equations was consistently positively correlated with age and frequently negatively correlated with FM. Bias and limits of agreement were similar among subgroups of patients.ConclusionREE cannot be accurately predicted on an individual level, and bias relates to age and FM.
Bevacizumab in treating the cystic components of pediatric low‐grade gliomas: A report of four patients
Authors: Levenbaum Erica , Ellika Shehanaz , Korones David N. 
Published: July 2019

AbstractLow‐grade gliomas (LGG) are among the most common types of brain tumors in children and young adults. These tumors often consist of solid and cystic components. Bevacizumab is a documented treatment for progressive LGG, yet the impact of therapy on the cystic component of these tumors is unknown. We present four patients with prominently cystic LGG treated with bevacizumab at the time of progression. In each case, the cystic component responded to treatment. This is the first known study to investigate bevacizumab's impact on the cystic component of low‐grade gliomas.
Efficacy and Safety of the Use of Pulmonary Arterial Hypertension Pharmacotherapy in Patients with Pulmonary Hypertension Secondary to Left Heart Disease: A Systematic Review
Authors: Kido Kazuhiko , Coons James C. 
Published: July 2019

Pulmonary hypertension (PH) is often caused by left heart disease (LHD) such as heart failure (HF) or valvular heart disease. Historically, few randomized controlled trials have evaluated the off‐label use of medications for treating pulmonary arterial hypertension (PAH) in patients with PH‐LHD. However, multiple randomized controlled trials have been published over the last decade that investigated their use in patients with PH‐LHD. In addition, recent updates in the classification and definitions of PH have led to an improved recognition of PH‐LHD phenotypes, notably combined post‐capillary and pre‐capillary PH and isolated post‐capillary PH. In this systematic review, we show that PAH medications should not be recommended in two distinct HF populations: patients with HF without definitive PH diagnosis and patients with isolated post‐capillary PH due to HF. In addition, the use of bosentan or macitentan is not recommended in patients with combined post‐capillary and pre‐capillary PH due to HF, but sildenafil may be considered to improve pulmonary hemodynamics and exercise capacity in patients with combined post‐capillary and pre‐capillary PH due to HF. Riociguat 2 mg 3 times daily may also be considered to improve pulmonary hemodynamics in patients with combined post‐capillary and pre‐capillary PH due to heart failure with reduced ejection fraction but not heart failure with preserved ejection fraction. The postoperative use of sildenafil in the setting of PH after valvular heart disease intervention was evaluated. Limited clinical data and safety concern warrants caution with the postoperative use of sildenafil in patients with PH due to valvular heart disease. Despite recent advances in the understanding of PAH medications for patients with PH‐LHD, uncertainty remains about their utility in distinct subgroups. Nonetheless, PAH pharmacotherapy should generally be avoided for most patients with PH‐LHD.
Accelerating structure‐function mapping using the ViVa webtool to mine natural variation
Authors: Hamm Morgan O. , Moss Britney L. , Leydon Alexander R. , Gala Hardik P. , Lanctot Amy , Ramos Román , Klaeser Hannah , Lemmex Andrew C. , Zahler Mollye L. , Nemhauser Jennifer L. , Wright R. Clay 
Published: July 2019

AbstractThousands of sequenced genomes are now publicly available capturing a significant amount of natural variation within plant species; yet, much of these data remain inaccessible to researchers without significant bioinformatics experience. Here, we present a webtool called ViVa (Visualizing Variation) which aims to empower any researcher to take advantage of the amazing genetic resource collected in the Arabidopsis thaliana 1001 Genomes Project (http://1001genomes.org). ViVa facilitates data mining on the gene, gene family, or gene network level. To test the utility and accessibility of ViVa, we assembled a team with a range of expertise within biology and bioinformatics to analyze the natural variation within the well‐studied nuclear auxin signaling pathway. Our analysis has provided further confirmation of existing knowledge and has also helped generate new hypotheses regarding this well‐studied pathway. These results highlight how natural variation could be used to generate and test hypotheses about less‐studied gene families and networks, especially when paired with biochemical and genetic characterization. ViVa is also readily extensible to databases of interspecific genetic variation in plants as well as other organisms, such as the 3,000 Rice Genomes Project ( http://snp-seek.irri.org/) and human genetic variation ( https://www.ncbi.nlm.nih.gov/clinvar/).
Perylene polyphenylmethylsiloxanes for optoelectronic applications
Authors: Steinbrück Nils , Kickelbick Guido 
Published: July 2019

ABSTRACTThe incorporation of fluorescent organic dyes in an encapsulating matrix represents a route to generate stable and processable materials for optoelectronic devices. Here, we present a method to embed perylene dyes into a high refractive index (HRI) polysiloxane matrix applying an allyl functionalized perylene dye and hydrosilylation chemistry. In a first approach, the dye molecules were covalently integrated into the backbone of linear polyphenylmethylsiloxane chains. The fluorescent and liquid polymers were synthesized with molecular weights from 5660 up to 8400 g mol−1. In a second approach, the dye itself was used as a cross‐linking agent between linear polyphenylmethylsiloxane chains. These preformed fluorescent batch polymers are liquids with dye concentrations between 0.025 and 8 wt %. The applied synthetic methods incorporated the dye covalently into the polymer structure and avoided the crystallization of the dye molecules and thus the formation of excimers, which would reduce the optical emission. The resulting products can be easily incorporated into curable commercially available HRI polyphenylmethylsiloxane resins. The formed materials are ideal LED encapsulants with a solid and flexible consistency, a uniform dispersion of the dyes, and adjustable mechanical properties, realized by changing the amount of perylene polymers. Further properties of the obtained materials are thermal stabilities up to 478 °C, quantum yields larger than 0.97, and high photostabilities. Thus, the covalent integration of dyes into polyphenylsiloxane structures represents a possible route for the stabilization of the organic dyes against the extreme irradiance and thermal conditions in LED applications. © 2019 The Authors. Journal of Polymer Science Part B: Polymer Physics published by Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2019, 57, 1062–1073