bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resilience Predicts Social Functioning in Clinically Stable Patients With Bipolar Disorder
Authors: Bozikas Vasilis P. , Parlapani Eleni , Ntouros Evangelos , Bargiota Stavroula I. , Floros Georgios , Nazlidou Elena I. , Garyfallos Georgios 
Published: June 2018

Abstract
The current study focused on the association between resilience and social functioning in patients with bipolar disorder (BD). Forty clinically stable patients with BD type I and BD type II, as well as 40 healthy controls, matched for age, sex, and educational background were enrolled. Clinical status was assessed by the Montgomery-Åsberg Depression Rating Scale and the Young Mania Rating Scale. Functioning was evaluated by the Mini International Classification of Functioning, Disability and Health rating of activities and participation in mental illnesses, and the Social and Occupational Functioning Scale. Resilience was measured by the Connor-Davidson Resilience scale. Patients demonstrated significantly lower resilience levels compared with healthy individuals. A stepwise regression analysis indicated that only resilience contributed significantly to social functioning's outcome. Because resilience may constitute a social functioning moderator in clinically stable patients with BD, a paradigm shift toward protective factors could lead to implementation of resilience-oriented interventions designed specifically for patients with BD.
Canine Outreach Promoting Engagement
Authors: Yordy B. Morgan , Pope W. Stuart , Wang Chih-Hsuan 
Published: June 2018

Background
Animal-assisted activities (AAAs) show promise in providing emotional and social benefits to older adults and may be used as a tool to promote therapeutic communication between students and cognitively impaired older adults.

Purpose
The purpose was to develop a program incorporating AAAs to enhance social engagement of cognitively impaired older adults in a community respite program and in turn enhance student comfort when caring for this vulnerable population.

Methods
The Dementia Attitudes Scale, a validated tool, was used to measure students’ attitudes before and after AAA intervention. Data were analyzed using repeated-measures analysis of variance.

Results
Students were significantly more comfortable and demonstrated a gain in knowledge after AAAs were included in the community clinical experience.

Conclusions
Incorporating AAAs into student community/service-learning clinical experience improved communication between students and cognitively impaired older adults, improving students’ attitudes when caring for this population.
Ocular Injury Presenting to a Level-III Pediatric Trauma Center
Authors: Schneider Kari , Nguyen-Tran Hai , Segura Bradley J. , Areaux Raymond G. , Nerheim Dan , Louie Jeffrey P. 
Published: June 2018

Objective
The purpose of this study is to describe pediatric ocular injuries presenting to a level-III pediatric trauma center and emergency department.

Methods
We performed a retrospective study and identified children from January 1, 2011, to January 1, 2016. Charts were reviewed for any subject, age from newborn to younger than 18 years, based on International Classification of Diseases, ninth and tenth revision, codes for any ocular injury. Data abstraction included age, sex, means of arrival, eye involved, mechanism of injury, type of ocular injury, imaging studies obtained, procedures performed, location of definitive repair (in the operating room or emergency department), and subspecialty services involved.

Results
In the 5-year period, we describe 356 injuries among 278 children. Males had a slightly higher rate of presentation than females (156 and 122, respectively). Forty-three children (15.46%) required repair in the operating room. Dog bites comprised of 7.19% children with outpatient follow-up, one patient (0.36%) eventually developed anophthalmia, and 30 children (10.79%) had long-term ophthalmological sequelae (ie, glaucoma and blindness).

Conclusions
At our institution, a level-III trauma center, we evaluated and managed approximately 1 ocular injury case per week and children required surgical repair in the operating room at a higher rate than higher-level trauma centers. Injuries secondary to dog bites remain a clinically significant etiology.
Stevens-Johnson Syndrome and Toxic Epidermal Necrolysis–Like Lupus Erythematosus
Authors: Tankunakorn Jutamas , Sawatwarakul Sirima , Vachiramon Vasanop , Chanprapaph Kumutnart 
Published: June 2018

Background/Objective
Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN)–like lupus erythematosus is a hyperacute and life-threatening form of cutaneous lupus erythematosus. Because of its rarity, little is known about this entity. We aimed to evaluate the clinical characteristics, laboratory findings, systemic manifestations, treatments, and outcome of SJS/ TEN-like lupus erythematosus.

Methods
We conducted a chart review study from July 2002 to September 2016 of all patients diagnosed with SJS/TEN-like lupus erythematosus who presented with gradual epidermal necrolysis without clear drug or infectious culprit. We evaluate for clinical features, extracutaneous involvement, Systemic Lupus Erythematosus Disease Activity Index, histologic findings, immunofluorescence pattern, serologic abnormalities, treatment, outcome, and recurrence of SJS/TEN-like lupus erythematosus.

Results
Of 9074 patients diagnosed with cutaneous lupus erythematosus and/or systemic lupus erythematosus, 6 patients justified the diagnosis of SJS/TEN-like lupus erythematosus (5 SJS/TEN-like acute cutaneous lupus erythematosus, 1 TEN-like subacute cutaneous lupus erythematosus), accounting for 0.07%. Fifty percent had epidermal necrolysis as the initial presentation of lupus with a median time from onset of 1.5 months (0–48 months). The median duration between initial rash and epidermal detachment was 4.5 days (3–14 days). All had internal organ involvement (hematologic and renal) and high Systemic Lupus Erythematosus Disease Activity Index score (median, 19.5 [16–24]). Most recovered with systemic corticosteroids, antimalarial drugs, and/or immunosuppressants. None had disease recurrence.

Conclusions
This is the largest single series of patients with SJS/TEN-like lupus erythematosus. Skin damage is an indicator of disease activity, and careful search for extracutaneous involvement to prevent further complications is mandatory.
Fibrinogen Protects Against Barrier Dysfunction Through Maintaining Cell Surface Syndecan-1 In Vitro
Authors: Wu Feng , Kozar Rosemary A. 
Published: June 2018

ABSTRACT

Background:
We have shown that fresh frozen plasma's (FFP) protection of pulmonary endothelial barrier integrity following hemorrhagic shock is due in part to restoration of endothelial syndecan-1. In the present study, we investigated the role of fibrinogen, a major component of FFP, as an endothelial protector and hypothesize that fibrinogen stabilizes cell surface syndecan-1 to restore endothelial barrier integrity.

Methods:
Pulmonary endothelial cells were incubated in FFP, fibrinogen, or lactated Ringers (LR) then immunostained with anti-syndecan-1 or fibrinogen and barrier integrity assessed. In some experiments, cells were exposed to fibrinogen depleted plasma.

Results:
Cell surface syndecan-1 was increased by FFP and fibrinogen compared with LR-treated cells while barrier integrity was augmented by FFP and fibrinogen compared with LR. The physiological concentration of 2.5 mg/mL fibrinogen was sufficient to increase cell surface syndecan-1. Colocalization and co-immunoprecipitation experiments demonstrated that fibrinogen associates with syndecan-1. Fibrinogen-deficient plasma was unable to augment sydnecan-1 immunostaining and lost its endothelial protective effect on barrier integrity.

Conclusion:
These data suggest that in vitro, fibrinogen associated with cell surface syndecan-1 and enhanced endothelial barrier integrity.
Spontaneous but not voluntary eye blinks during spatial working memory are associated with successful performance
Authors: Lee Andrew S. , Blankson Baaba K. , Manza Peter , O’Rawe Jonathan F. , Evinger Craig , Leung Hoi-Chung 
Published: June 2018

AbstractSpontaneous eye blink rate (SBR) has been associated with central dopamine (DA) levels, raising the intriguing possibility that SBR is related to cognitive functions dependent on DA, such as spatial working memory (WM). We tested this hypothesis in two behavioral experiments, examining the relationship between SBR, WM load and individual differences in spatial WM performance in 126 young adults. In Experiment 1, we examined the temporal profile of SBR during a spatial delayed recognition task requiring maintenance of 1, 2, 4, 6 or 7 dot locations. We observed a suppression in SBR during dot- and recognition probe-presentation, and a significant increase in SBR afterwards. High performers showed significantly lower SBR than low performers during the first 500 ms of the delay period. In Experiment 2, we used a similar spatial WM task as Experiment 1 to test whether an instructed voluntary blink during the early delay would directly dampen WM performance. While the temporal dynamics of SBR across task events were comparable to Experiment 1, WM performance was not significantly different between the voluntary blink and no blink conditions. Together, these results suggest that spontaneous but not voluntary eye blinking is closely linked to spatial WM, and that lower SBR during WM encoding and early phase of maintenance is associated with better WM task performance.
IQGAP3 Overexpression Correlates with Poor Prognosis and Radiation Therapy Resistance in Breast Cancer
Authors: Hua Xin , Long Zhi-Qing , Zhang Wen-Wen , Lin Chao , Sun Xiao-Qing , Wen Wen , Lu Zi-Jian , Guo Na , He Zhen-Yu , Bing-Song Li , Guo Ling , Lin Huan-Xin 
Published: June 2018

AbstractBackground: IQ motif-containing GTPase activating protein 3 (IQGAP3), the latest found protein of IQGAP family, may act as a crucial factor in the process of cancer development and progression; however, its clinical value in breast cancer remains unestablished so far. Our team explored the correlation between IQGAP3 expression profile and the clinicopathological features in breast cancer. Methods: IQGAP3 levels in breast cancer cell lines and tumor tissues were detected by real-time PCR and western blotting and compared to the normal control groups. Protein expression of IQGAP3 was evaluated immunohistochemically in specimens (archived paraffin embedded) of 257 breast cancer patients. We also analyze the association between IQGAP3 expression and the clinical characters and prognosis. The relationship between IQGAP3 expression and sensitivity to radiation therapy was determined by subgroup analysis. Results: There was significant upregulation of IQGAP3 in breast cancer cell lines and human tumor tissues at both the mRNA and protein level compared to the normal ones. In addition, 110/257 (42.8%) of archived paraffin embedded breast cancer specimens had high protein expression of IQGAP3. High expression of IQGAP3 was significantly related to clinical stage (P=0.001), T category (P=0.002), N category (P=0.001), locoregional recurrence(P=0.002), distant metastasis (P=0.001), and vital status (P=0.001). Univariate and multivariate statistical analysis showed that IQGAP3 was an independent prognostic factor of the whole cohort breast cancer patients (P=0.003, P=0.001). Subgroup analysis revealed IQGAP3 expression correlates with radiation therapy resistance and was also an independent predictor for radiation therapy outcome. Conclusions: Our findings suggest that high IQGAP3 expression predicts poor prognosis and radiation therapy resistance in breast cancer. In addition, IQGAP3 may be a reliable novel biomarker to provide personalized prognostication and identify patients who can profit from more aggressive RT regimen for improving the survival of breast cancer patients.
Dynamics and predicted drug response of a gene network linking dedifferentiation with β-catenin dysfunction in hepatocellular carcinoma
Authors: Gérard Claude , Di-Luoffo Mickaël , Gonay Léolo , Caruso Stefano , Couchy Gabrielle , Loriot Axelle , Tao Junyan , Konobrocka Katarzyna , Cordi Sabine , Monga Satdarshan P. , Hanert Emmanuel , Zucman-Rossi Jessica , Lemaigre Frédéric P. 
Published: June 2018

AbstractAlterations of individual genes variably affect development of hepatocellular carcinoma (HCC), prompting the need to characterize the function of tumor-promoting genes in the context of gene regulatory networks (GRN). Here, we identify a GRN which functionally links LIN28B-dependent dedifferentiation with dysfunction of CTNNB1 (β-CATENIN). LIN28B and CTNNB1 form a functional GRN with SMARCA4 (BRG1), Let-7b, SOX9, TP53 and MYC. GRN activity is detected in HCC and gastrointestinal cancers; it negatively correlates with HCC prognosis and contributes to a transcriptomic profile typical of the proliferative class of HCC. Using data from The Cancer Genome Atlas and from transcriptomic, transfection and mouse transgenic experiments, we generated and validated a quantitative mathematical model of the GRN. The model predicts how the expression of GRN components changes when the expression of another GRN member varies or is inhibited by a pharmacological drug. The dynamics of GRN component expression reveal distinct cell states that can switch reversibly in normal condition, and irreversibly in HCC. We conclude that identification and modelling of the GRN provides insight into prognosis, mechanisms of tumor-promoting genes and response to pharmacological agents in HCC.
SIV/SHIV-Zika coinfection does not alter disease pathogenesis in adult non-pregnant Rhesus Macaques
Authors: Bidokhti Mehdi R. M. , Dutta Debashis , Madduri Lepakshe S. V. , Woollard Shawna M. , Norgren Robert , Giavedoni Luis , Byrareddy Siddappa N. 
Published: June 2018

AbstractDue to the large geographical overlap of populations exposed to Zika virus (ZIKV) and human immunodeficiency virus (HIV), understanding disease pathogenesis in such coinfections is urgently needed. We used chronically infected simian immunodeficiency virus and chimeric simian human immunodeficiency virus (SIV/SHIV) macaques and inoculated with ZIKV. Plasma viral loads of both SIV/SHIV and ZIKV showed no significant changes as compared to ZIKV alone-infected animals. Tissue clearance of ZIKV was observed similarly. Furthermore, minimal changes in cytokines/chemokines were observed. Collectively, these data suggest that coinfection may not alter disease pathogenesis and warrants large HIV-ZIKV epidemiological studies to validate these findings.Author SummaryThe co-infection incidence of human immunodeficiency virus (HIV) infection and neglected tropical infectious diseases is increasing due to the large geographical overlap of populations exposed to both of these viruses. Thus, researching on such coinfection is of particular importance. In this study, we investigated HIV-ZIKV coinfection dynamics in adult non-pregnant Rhesus Macaques model chronically infected with simian immunodeficiency virus (SIV) - or chimeric simian human immunodeficiency virus (SHIV). We found that post ZIKV inoculation, plasma viral loads were similar to ZIKV alone infected animals in addition to minimal changes of cytokines. Dynamics of SIV and SHIV also did not change. Tissue clearance of ZIKV was found 67 months later. Our findings provide insights into HIV-ZKIV coinfection to determine the alteration of their pathogenesis.
PennZzz - an algorithm for estimating behavioral states from wrist-worn accelerometery
Authors: McCloskey Richard , Goodman Matthew H. , Grant Sara McHugh , Ghorai Arpita , Gehrman Philip R. , Bućan Maja 
Published: June 2018

AbstractSleep is a heterogeneous behavioral state comprised of different stages and interspersed with episodes of wakefulness. Sleep/wake states can be monitored in the sleep laboratory by polysomnography (PSG). However, sleep studies are intrusive, laborious and expensive, and are usually performed over a single night. In contrast, wrist-worn activity-tracking devices (actimeters) are inexpensive, unobtrusive, and can be used to estimate sleep and wake patterns over multiple nights. We designed the PennZzz algorithm to estimate sleep and wake from actimetry data. Results obtained by actimetry-based monitoring in 26 subjects were compared to stages of sleep and wakefulness detected by simultaneous polysomnography. We found that our algorithm identifies PSG-defined wake episodes with a high accuracy (336/431 – 76% of algorithm wake events correspond to true wakefulness). Furthermore, we find that the algorithm is sensitive enough to detect the majority (258/431 – 59%) of true wake episodes occurring after the first NREM1 to NREM2 transition. With correction, algorithm outputs can be used to estimate the total amount of time awake after sleep onset. We further refined this program for application in a high-throughput manner to assess the total amount of sleep, wake, and non-wear during longer recording periods.
Synthesis of three major auxins from glucose in EngineeredEscherichia coli
Authors: Guo Daoyi , Zhang Lihua , Kong Sijia , Liu Zhijie , Chu Xu , Li Xun , Pan Hong 
Published: June 2018

ABSTRACTIndole-3-acetic acid (IAA) is considered the most common and important naturally occurring auxin in plants and a major regulator of plant growth and development. In addition, phenylacetic acid (PAA) and 4-hydroxyphenylacetic acid (4HPA) can also play a role as auxin in some plants. In recent years, several microbes have been metabolically engineered to produce IAA from L-tryptophan. In this study, we showed that aminotransferasearo8and decarboxylasekdcfromSaccharomyces cerevisiae, and aldehyde dehydrogenasealdHfromEscherichia colihave broad substrate ranges and can catalyze the conversion of three kinds of aromatic amino acids (L-tryptophan, L-tyrosine or L-phenylalanine) to the corresponding IAA, 4HPA and PAA. Subsequently, three de novo biosynthetic pathways for the production of IAA, PAA and 4HPA from glucose were constructed inE. colithrough strengthening the shikimate pathway. This study described here shows the way for the development of agricultural microorganism for biosynthesis of plant auxin and promoting plant growth in the future.
Interactions in self-assembled microbial communities saturate with diversity
Authors: Yu Xiaoqian , Polz Martin F. , Alm Eric J. 
Published: June 2018

AbstractHow the diversity of organisms competing for or sharing resources influences community production is an important question in ecology but has rarely been explored in natural microbial communities. These generally contain large numbers of species making it difficult to disentangle how the effects of different interactions scale with diversity. Here, we show that changing diversity affects measures of community function in relatively simple communities but that increasing richness beyond a threshold has little detectable effect. We generated self-assembled communities with a wide range of diversity by growth of cells from serially diluted seawater on brown algal leachate. We subsequently isolated the most abundant taxa from these communities via dilution-to-extinction in order to compare productivity functions of the entire community to those of individual taxa. To parse the effect of different types of organismal interactions, we developed relative total function (RTF) as an index for positive or negative effects of diversity on community function. Our analysis identified three overall regimes with increasing diversity. At low richness (<12 taxa), potential positive and negative effects of interactions are both weak, while at moderate richness (12-20 taxa), community resource uptake increases but the carbon use efficiency decreases. Finally, beyond 20 taxa, there was no net change in community function indicating a saturation of potential interactions. These data suggest that although more diverse communities had overall greater access to resources, individual taxa within these communities had lower resource availability and reduced carbon use efficiency, indicating that competition due to niche overlap increases with diversity but that these interactions saturate at a specific threshold.
Endothelial Protein C Receptor Could Contribute to Experimental Malaria-Associated Acute Respiratory Distress Syndrome
Authors: Santos Ortolan Luana dos , Sercundes Michelle Klein , Moura Gabriel Candido , de Castro Quirino Thatyane , Debone Daniela , de Sousa Costa Douglas , Murillo Oscar , Marinho Claudio Romero Farias , Epiphanio Sabrina 
Published: June 2018

AbstractThe severity of Plasmodium falciparum malaria is associated with parasite cytoadherence, but there is limited knowledge about the effect of parasite cytoadherence in malaria-associated acute respiratory distress syndrome (ARDS). Our objective was to evaluate the cytoadherence of infected red blood cells (iRBCs) in a murine model of ARDS and to appraise a potential function of endothelial protein C receptor (EPCR) in ARDS pathogenesis. DBA/2 mice infected with P. berghei ANKA were classified as ARDS- or hyperparasitemia (HP)-developing mice according to respiratory parameters and parasitemia. Lungs, blood and bronchoalveolar lavage were collected for gene expression or protein analyses. Primary cultures of microvascular lung endothelial cells from DBA/2 mice were analyzed for iRBC interactions. Lungs from ARDS-developing mice showed evidence of iRBC accumulation along with an increase in EPCR and TNF concentrations. Furthermore, TNF increased iRBC adherence in vitro. Dexamethasone-treated infected mice showed low levels of TNF and EPCR mRNA expression and, finally, decreased vascular permeability, thus protecting mice from ARDS. In conclusion, we identified that increased iRBC cytoadherence in the lungs underlies malaria-associated ARDS in DBA/2-infected mice and that inflammation increased cytoadherence capacity, suggesting a participation of EPCR and a conceivable target for drug development.
Effects of MHD mixed convection with non-uniform heat source/sink and cross-diffusion over exponentially stretching sheet
Authors: Patil Prabhugouda Mallanagouda , Kumbarwadi Nafisabanu , A. Shashikant 
Published: June 2018

Purpose
The purpose of this paper is to investigate the magnetohydrodynamics mixed convection flow over an exponentially stretching surface in the presence of non-uniform heat source/sink and cross-diffusion. Adequate non-similar transformations are used to transform governing mixed convection boundary layer equations to dimensionless form.

Design/methodology/approach
These dimensionless partial differential equations are solved by using implicit finite difference scheme in conjunction with Quasi-linearization technique.

Findings
The effects of admissible parameters such as Eckert number (Ec), the ratio of buoyancy forces parameter (N), non-uniform heat source/sink, Soret and Dufour numbers on flow, temperature and concentration distributions are discussed and analysed through graphs. In addition, the results for skin friction coefficient, Sherwood number and Nusselt number are presented and discussed graphically.

Originality/value
In literature, no research work has been found in similar to this research paper.