bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infralimbic cortex is required for learning alternatives to prelimbic promoted associations through reciprocal connectivity
Authors: Mukherjee Arghya , Caroni Pico 
Published: July 2018

AbstractPrefrontal cortical areas mediate flexible adaptive control of behavior, but the specific contributions of individual areas and the circuit mechanisms through which they interact to modulate learning have remained poorly understood. Using viral tracing and pharmacogenetic techniques, we show that prelimbic (PreL) and infralimbic cortex (IL) exhibit reciprocal PreL↔IL layer 5/6 connectivity. In set-shifting tasks and in fear/extinction learning, activity in PreL is required during new learning to apply previously learned associations, whereas activity in IL is required to learn associations alternative to previous ones. IL→PreL connectivity is specifically required during IL-dependent learning, whereas reciprocal PreL↔IL connectivity is required during a time window of 12–14 h after association learning, to set up the role of IL in subsequent learning. Our results define specific and opposing roles of PreL and IL to together flexibly support new learning, and provide circuit evidence that IL-mediated learning of alternative associations depends on direct reciprocal PreL↔IL connectivity.
Unusual evolution of Bc2 and Tc with inclined fields in restacked TaS2 nanosheets
Authors: Ma Yonghui , Pan Jie , Guo Chenguang , Zhang Xuan , Wang Lingling , Hu Tao , Mu Gang , Huang Fuqiang , Xie Xiaoming 
Published: July 2018

AbstractRecently we reported an enhanced superconductivity in restacked monolayer TaS2 nanosheets compared with the bulk TaS2, pointing to the exotic physical properties of low dimensional systems. Here we tune the superconducting properties of this system with magnetic field along different directions, where a strong Pauli paramagnetic spin-splitting effect is found in this system. Importantly, an unusual enhancement as high as 3.8 times of the upper critical field Bc2, as compered with the Ginzburg-Landau (GL) model and Tinkham model, is observed under the inclined external magnetic field. Moreover, with the out-of-plane field fixed, we find that the superconducting transition temperature Tc can be enhanced by increasing the in-plane field and forms a dome-shaped phase diagram. An extended GL model considering the special microstructure with wrinkles was proposed to describe the results. The restacked crystal structure without inversion center along with the strong spin–orbit coupling may also play an important role for our observations.
Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice
Authors: Michaudel Chloé , Fauconnier Louis , Julé Yvon , Ryffel Bernhard 
Published: July 2018

AbstractEnvironmental air pollutants including ozone cause severe lung injury and aggravate respiratory diseases such as asthma and COPD. Here we compared the effect of ozone on respiratory epithelium injury, inflammation, hyperreactivity and airway remodeling in mice upon acute (1ppm, 1 h) and chronic exposure (1.5ppm, 2 h, twice weekly for 6 weeks). Acute ozone exposure caused respiratory epithelial disruption with protein leak and neutrophil recruitment in the broncho-alveolar space, leading to lung inflammation and airway hyperresponsiveness (AHR) to methacholine. All these parameters were increased upon chronic ozone exposure, including collagen deposition. The structure of the airways as assessed by automatic numerical image analysis showed significant differences: While acute ozone exposure increased bronchial and lumen circularity but decreased epithelial thickness and area, chronic ozone exposure revealed epithelial injury with reduced height, distended bronchioles, enlarged alveolar space and increased collagen deposition, indicative of peribronchiolar fibrosis and emphysema as characterized by a significant increase in the density and diameter of airspaces with decreased airspace numbers. In conclusion, morphometric numerical analysis enables an automatic and unbiased assessment of small airway remodeling. The structural changes of the small airways correlated with functional changes allowing to follow the progression from acute to chronic ozone induced respiratory pathology.
The genetic structure of Squalidus multimaculatus revealing the historical pattern of serial colonization on the tip of East Asian continent
Authors: Jeon Hyung-Bae , Kim Dong-Young , Lee Yoon Jeong , Bae Han-Gyu , Suk Ho Young 
Published: July 2018

AbstractSeparated river systems could create confluences via two geological processes, estuary coalescence in response to decreasing sea levels and headwater capture, allowing primary freshwater species to disperse across rivers. Squalidus multimaculatus, is an endemic and primary freshwater species restricted to the southeast coast of the Korean Peninsula. The distribution of this species is unique, given that other congeneric species, including its closely related S. gracilis majimae, as well as other cyprind species are observed throughout the peninsula except for the east coast. Phylogeographic analyses were conducted using three mitochondrial loci to identify the origin of S. multimaculatus and the historical pathways of dispersal. A strong phylogenetic affinity between S. multimaculatus and S. g. majimae and the genetic structure among populations indicated that S. multimaculatus originated from the eastward colonization of the common ancestor between S. g. majimae and S. multimaculatus via headwater capture through fault zones within successive mountain range. Following colonization, the ancestral S. multimaculatus likely migrated towards north via estuary coalescence along a well-developed continental shelf. Our study was the first empirical attempt providing insights into how freshwater organisms dispersed to the southernmost tip of East Asia, despite the potential loss of such historical imprints with anthropogenic interference.
On the role of ocular torsion in binocular visual matching
Authors: Hess Bernhard J. M. 
Published: July 2018

AbstractWhen an observer scans the visual surround, the images cast on the two retinae are slightly different due to the different viewpoints of the two eyes. Objects in the horizontal plane of regard can be seen single by aligning the lines of sight without changing the torsional stance of the eyes. Due to the peculiar ocular kinematics this is not possible for objects above or below the horizontal plane of regard. We provide evidence that binocular fusion can be achieved independently of viewing direction by adjusting the mutual torsional orientation of the eyes in the frontal plane. We characterize the fusion positions of the eyes across the oculomotor range by deriving simple trigonometric equations for the required torsion as a function of gaze direction and compute the iso-torsion contours yielding binocular fusion. Finally, we provide experimental evidence that eye positions in far-to-near re-fixation saccades indeed converge towards the predicted positions by adjusting the torsion of the eyes. This is the first report that describes the three-dimensional orientation of the eyes at binocular fusion positions based on the three-dimensional ocular kinematics. It closes a gap between the sensory and the motor side of binocular vision and stereoscopy.
Structure and energy based quantitative missense variant effect analysis provides insights into drug resistance mechanisms of anaplastic lymphoma kinase mutations
Authors: Li Jianzong , Huang Yue , Wu Miaomiao , Wu Chuanfang , Li Xin , Bao Jinku 
Published: July 2018

AbstractAnaplastic lymphoma kinase (ALK) is considered as a validated molecular target in multiple malignancies, such as non-small cell lung cancer (NSCLC). However, the effectiveness of molecularly targeted therapies using ALK inhibitors is almost universally limited by drug resistance. Drug resistance to molecularly targeted therapies has now become a major obstacle to effective cancer treatment and personalized medicine. It is of particular importance to provide an improved understanding on the mechanisms of resistance of ALK inhibitors, thus rational new therapeutic strategies can be developed to combat resistance. We used state-of-the-art computational approaches to systematically explore the mutational effects of ALK mutations on drug resistance properties. We found the activation of ALK was increased by substitution with destabilizing mutations, creating the capacity to confer drug resistance to inhibitors. In addition, results implied that evolutionary constraints might affect the drug resistance properties. Moreover, an extensive profile of drugs against ALK mutations was constructed to give better understanding of the mechanism of drug resistance based on structural transitions and energetic variation. Our work hopes to provide an up-to-date mechanistic framework for understanding the mechanisms of drug resistance induced by ALK mutations, thus tailor treatment decisions after the emergence of resistance in ALK-dependent diseases.
Entanglement Availability Differentiation Service for the Quantum Internet
Authors: Gyongyosi Laszlo , Imre Sandor 
Published: July 2018

AbstractA fundamental concept of the quantum Internet is quantum entanglement. In a quantum Internet scenario where the legal users of the network have different priority levels or where a differentiation of entanglement availability between the users is a necessity, an entanglement availability service is essential. Here we define the entanglement availability differentiation (EAD) service for the quantum Internet. In the proposed EAD framework, the differentiation is either made in the amount of entanglement with respect to the relative entropy of entanglement associated with the legal users, or in the time domain with respect to the amount of time that is required to establish a maximally entangled system between the legal parties. The framework provides an efficient and easily-implementable solution for the differentiation of entanglement availability in experimental quantum networking scenarios.
Comparison of oxidation in uni-directionally and randomly oriented Cu films for low temperature Cu-to-Cu direct bonding
Authors: Tseng Chih-Han , Tu K. N. , Chen Chih 
Published: July 2018

AbstractCu-to-Cu direct bonding has attracted attention because it has been implemented in CMOS image sensors. Prior to the bonding, the oxides on the Cu surface needs to be removed, yet the surface may oxidize right after cleaning. Thus, oxidation is an inherent issue in the application of Cu direct bonding. Our previous study reported that Cu direct bonding can be achieved below 250 °C by using (111)-oriented nanotwinned Cu because it has the fastest surface diffusivity. However, the oxidation behavior of the nanotwinned Cu is unclear. Here, we examined the oxidation behavior of highly (111) and (200) oriented, and randomly-oriented Cu films at temperatures ranging from 120 to 250 °C. Transmission electron microscopy was used to measure the oxide thickness. The results show that the oxidation rate of (111)-oriented nanotwinned Cu has the lowest oxidation rate among them. Together, it is unique to possess the combination of the fastest surface diffusivity and the lowest oxidation rate.
CD200-CD200R imbalance correlates with microglia and pro-inflammatory activation in rat spinal cords exposed to amniotic fluid in retinoic acid-induced spina bifida
Authors: Oria Marc , Figueira Rebeca L. , Scorletti Federico , Sbragia Lourenco , Owens Kathryn , Li Zhen , Pathak Bedika , Corona Maria U. , Marotta Mario , Encinas Jose L. , Peiro Jose L. 
Published: July 2018

AbstractSpina bifida aperta is a congenital malformation characterized by the failure of neural tube closure resulting in an unprotected fetal spinal cord. The spinal cord then undergoes progressive damage, likely due to chemical and mechanical factors related to exposure to the intrauterine environment. Astrogliosis in exposed spinal cords has been described in animal models of spina bifida during embryonic life but its relationship with neuroinflammatory processes are completely unknown. Using a retinoic acid-induced rat model of spina bifida we demonstrated that, when exposed to amniotic fluid, fetal spinal cords showed progressive astrogliosis with neuronal loss at mid-gestation (E15) compared to unexposed spinal cords. The number of microglial cells with a reactive phenotype and activation marker expression increased during gestation and exhibited progressive disruption in the inhibitory immune ligand-receptor system. Specifically we demonstrate down-regulation of CD200 expression and up-regulation of CD200R. Exposed spinal cords demonstrated neuroinflammation with increased tissue water content and cytokine production by the end of gestation (E20), which correlated with active Caspase3 expression in the exposed layers. Our findings provide new evidence that microglia activation, including the disruption of the endogenous inhibitory system (CD200-CD200R), may participate in the pathogenesis of spina bifida through late gestation.
Exploring the clinical features of narcolepsy type 1 versus narcolepsy type 2 from European Narcolepsy Network database with machine learning
Authors: Zhang Zhongxing , Mayer Geert , Dauvilliers Yves , Plazzi Giuseppe , Pizza Fabio , Fronczek Rolf , Santamaria Joan , Partinen Markku , Overeem Sebastiaan , Peraita-Adrados Rosa , da Silva Antonio Martins , Sonka Karel , Rio-Villegas Rafael del , Heinzer Raphael , Wierzbicka Aleksandra , Young Peter , Högl Birgit , Bassetti Claudio L. , Manconi Mauro , Feketeova Eva , Mathis Johannes , Paiva Teresa , Canellas Francesca , Lecendreux Michel , Baumann Christian R. , Barateau Lucie , Pesenti Carole , Antelmi Elena , Gaig Carles , Iranzo Alex , Lillo-Triguero Laura , Medrano-Martínez Pablo , Haba-Rubio José , Gorban Corina , Luca Gianina , Lammers Gert Jan , Khatami Ramin 
Published: July 2018

AbstractNarcolepsy is a rare life-long disease that exists in two forms, narcolepsy type-1 (NT1) or type-2 (NT2), but only NT1 is accepted as clearly defined entity. Both types of narcolepsies belong to the group of central hypersomnias (CH), a spectrum of poorly defined diseases with excessive daytime sleepiness as a core feature. Due to the considerable overlap of symptoms and the rarity of the diseases, it is difficult to identify distinct phenotypes of CH. Machine learning (ML) can help to identify phenotypes as it learns to recognize clinical features invisible for humans. Here we apply ML to data from the huge European Narcolepsy Network (EU-NN) that contains hundreds of mixed features of narcolepsy making it difficult to analyze with classical statistics. Stochastic gradient boosting, a supervised learning model with built-in feature selection, results in high performances in testing set. While cataplexy features are recognized as the most influential predictors, machine find additional features, e.g. mean rapid-eye-movement sleep latency of multiple sleep latency test contributes to classify NT1 and NT2 as confirmed by classical statistical analysis. Our results suggest ML can identify features of CH on machine scale from complex databases, thus providing ‘ideas’ and promising candidates for future diagnostic classifications.
Cell surface nucleolin interacts with and internalizes Bothrops asper Lys49 phospholipase A2 and mediates its toxic activity
Authors: Massimino Maria Lina , Simonato Morena , Spolaore Barbara , Franchin Cinzia , Arrigoni Giorgio , Marin Oriano , Monturiol-Gross Laura , Fernández Julián , Lomonte Bruno , Tonello Fiorella 
Published: July 2018

AbstractPhospholipases A2 are a major component of snake venoms. Some of them cause severe muscle necrosis through an unknown mechanism. Phospholipid hydrolysis is a possible explanation of their toxic action, but catalytic and toxic properties of PLA2s are not directly connected. In addition, viperid venoms contain PLA2-like proteins, which are very toxic even if they lack catalytic activity due to a critical mutation in position 49. In this work, the PLA2-like Bothrops asper myotoxin-II, conjugated with the fluorophore TAMRA, was found to be internalized in mouse myotubes, and in RAW264.7 cells. Through experiments of protein fishing and mass spectrometry analysis, using biotinylated Mt-II as bait, we found fifteen proteins interacting with the toxin and among them nucleolin, a nucleolar protein present also on cell surface. By means of confocal microscopy, Mt-II and nucleolin were shown to colocalise, at 4 °C, on cell membrane where they form Congo-red sensitive assemblies, while at 37 °C, 20 minutes after the intoxication, they colocalise in intracellular spots going from plasmatic membrane to paranuclear and nuclear area. Finally, nucleolin antagonists were found to inhibit the Mt-II internalization and toxic activity and were used to identify the nucleolin regions involved in the interaction with the toxin.
Non-invasive prediction of NAFLD severity: a comprehensive, independent validation of previously postulated serum microRNA biomarkers
Authors: López-Riera Mireia , Conde Isabel , Quintas Guillermo , Pedrola Laia , Zaragoza Ángela , Perez-Rojas Judith , Salcedo Mario , Benlloch Salvador , Castell José V. , Jover Ramiro 
Published: July 2018

AbstractLiver biopsy is currently the only reliable method to establish nonalcoholic fatty liver disease (NAFLD) severity. However, this technique is invasive and occasionally associated with severe complications. Thus, non-invasive diagnostic markers for NAFLD are needed. Former studies have postulated 18 different serum microRNA biomarkers with altered levels in NAFLD patients. In the present study, we have re-examined the predictive value of these serum microRNAs and found that 9 of them (miR-34a, -192, -27b, -122, -22, -21, -197, -30c and -16) associated to NAFLD severity in our independent cohort. Moreover, miR-192, -27b, -22, -197 and -30c appeared specific for NAFLD, when compared with patients with drug-induced liver injury. Preliminary serum RNAseq analysis allowed identifying novel potential miRNA biomarkers for nonalcoholic steatohepatitis (NASH). The classification performance of validated miRNAs (and their ratios) for NASH was better than that reached by AST, whereas for advanced fibrosis prediction miRNAs did not perform better than the FIB-4 algorithm. Cross-validated models combining both clinical and miRNA variables showed enhanced predictivity. In conclusion, the circulating microRNAs validated demonstrate a better diagnostic potential than conventional serum markers to identify NASH patients and could complement and improve current fibrosis prediction algorithms. The research in this field is still open.
Probing disorder in pyrochlore oxides using in situ synchrotron diffraction from levitated solids–A thermodynamic perspective
Authors: Maram Pardha S. , Ushakov Sergey V. , Weber Richard J. K. , Benmore Chris J. , Navrotsky Alexandra 
Published: July 2018

AbstractPyrochlore, an ordered derivative of the defect fluorite structure, shows complex disordering behavior as a function of composition, temperature, pressure, and radiation damage. We propose a thermodynamic model to calculate the disordering enthalpies for several RE2Zr2O7 (RE = Sm, Eu, Gd) pyrochlores from experimental site distribution data obtained by in situ high temperature synchrotron X-ray diffraction. Site occupancies show a gradual increase in disorder on both cation and anion sublattices with increasing temperature and even greater disorder is achieved close to the phase transition to defect fluorite. The enthalpy associated with cation disorder depends on the radius of the rare earth ion, while the enthalpy of oxygen disordering is relatively constant for different compositions. The experimental data support trends predicted by ab initio calculations, but the obtained enthalpies of disordering are less endothermic than the predicted values. Thermal expansion coefficients are in the range (8.6–10.8) × 10−6 K−1. These new experimental determinations of defect formation energies are important for understanding the stability of pyrochlore oxides and their disordering mechanisms, which are essential in the context of their potential applications in nuclear waste management and other technologies.
Wnt7a induces satellite cell expansion, myofiber hyperplasia and hypertrophy in rat craniofacial muscle
Authors: Cheng Xu , Huang Hanyao , Luo Xiangyou , Shi Bing , Li Jingtao 
Published: July 2018

AbstractCraniofacial muscles drive critical functions in the head, including speech, feeding and expression. Compared with their counterparts in trunk and limbs, craniofacial muscles are of distinct embryonic origins, which might consequently lead to different growth patterns and regenerative potential. In this study, rat levator veli palatini muscle and masseter muscle were compared with tibialis anterior muscle in their response to exogenous Wnt7a stimulus, which has been proved effective in promoting muscle regeneration in the limbs. Histological, cellular and molecular analyses were performed both under basal condition and after a single dose injection of recombinant human Wnt7a. Under basal condition, levator veli palatini muscle demonstrated considerably more satellite cells than the others. After Wnt7a administration, regeneration-related activities, including satellite cell expansion, myofiber hyperplasia and hypertrophy were generally observed in all three muscles, but with obvious differences in the extent. The composition of fast/slow myofibers underwent substantial alterations, and the pattern varied among the three muscles. Location-specific alterations in the expression level of core components in planar cell polarity pathway, Akt/mTOR pathway and myostatin pathway were also observed. In conclusion, both craniofacial and limb muscles could be effectively expanded by exogenous Wnt7a stimulus, but muscle-to-muscle variations in response patterns existed.
Vigour of self-paced reaching movement: cost of time and individual traits
Authors: Berret Bastien , Castanier Carole , Bastide Simon , Deroche Thomas 
Published: July 2018

AbstractPeople usually move at a self-selected pace in everyday life. Yet, the principles underlying the formation of human movement vigour remain unclear, particularly in view of intriguing inter-individual variability. It has been hypothesized that how the brain values time may be the cornerstone of such differences, beyond biomechanics. Here, we focused on the vigour of self-paced reaching movement and assessed the stability of vigour via repeated measurements within participants. We used an optimal control methodology to identify a cost of time (CoT) function underlying each participant’s vigour, considering a model of the biomechanical cost of movement. We then tested the extent to which anthropometric or psychological traits, namely boredom proneness and impulsivity, could account for a significant part of inter-individual variance in vigour and CoT parameters. Our findings show that the vigour of reaching is largely idiosyncratic and tend to corroborate a relation between the relative steepness of the identified CoT and boredom proneness, a psychological trait relevant to one’s relationship with time in decision-making.