bluuebunny/crossref_metadata_2025_split huggingface dataset:
An integrated transcriptomic analysis of brain aging and strategies for healthy aging
Authors: Liu Haiying , Nie Xin , Wang Fengwei , Chen Dandan , Zeng Zhuo , Shu Peng , Huang Junjiu 
Published: December 2024

BackgroundIt is been noted that the expression levels of numerous genes undergo changes as individuals age, and aging stands as a primary factor contributing to age-related diseases. Nevertheless, it remains uncertain whether there are common aging genes across organs or tissues, and whether these aging genes play a pivotal role in the development of age-related diseases.MethodsIn this study, we screened for aging genes using RNAseq data of 32 human tissues from GTEx. RNAseq datasets from GEO were used to study whether aging genes drives age-related diseases, or whether anti-aging solutions could reverse aging gene expression.ResultsAging transcriptome alterations showed that brain aging differ significantly from the rest of the body, furthermore, brain tissues were divided into four group according to their aging transcriptome alterations. Numerous genes were downregulated during brain aging, with functions enriched in synaptic function, ubiquitination, mitochondrial translation and autophagy. Transcriptome analysis of age-related diseases and retarding aging solutions showed that downregulated aging genes in the hippocampus further downregulation in Alzheimer’s disease but were effectively reversed by high physical activity. Furthermore, the neuron loss observed during aging was reversed by high physical activity.ConclusionThe downregulation of many genes is a major contributor to brain aging and neurodegeneration. High levels of physical activity have been shown to effectively reactivate these genes, making it a promising strategy to slow brain aging.
Treating early-stage centrally-located non-small cell lung cancer with DCAT-SBRT in centers lacking the VMAT technique: a comprehensive study
Authors: Huang Yangyang , Yang Jun , Song Rui , Qin Tingting , Yang Menglin , Liu Yibao 
Published: December 2024

BackgroundVolumetric-modulated arc therapy (VMAT) may have the highest overall performance for stereotactic body radiotherapy (SBRT) treatment of inoperable early-stage NSCLC. However, in centers lacking the VMAT technique, the dynamic conformal arc therapy (DCAT) technique is potentially the best option for small and rounded NSCLC-SBRT. Therefore, we will comprehensively analyze the advantages of the DCAT versus the other techniques except VMAT in terms of dosimetry, plan complexity, delivery time, γ-passing rates and the interplay effect.Methods36 patients with early-stage centrally located NSCLC with PTV volumes &lt; 65 cc were enrolled. All patients were redesigned with 50Gy/5f, and 100% of the prescribed dose was normalized to cover 95% of the PTV. The other two delivery techniques compared to the DCAT technique include 3-dimensional conformal radiotherapy (3DCRT) and intensity-modulated radiotherapy (IMRT), which use the same parameters for all three techniques.ResultsThe dosimetric parameters of the 3-group plans all met the RTOG 0813 protocol. Unsurprisingly, plan complexity parameters such as segments and MUs were significantly reduced in the DCAT plans by 159.56 and 925.90 compared to the IMRT plans, respectively (all P &lt; 0.001). The delivery time of the DCAT plans was the least of 164.51 s (all P &lt; 0.05). Compared to the IMRT plans, the γ-passing rates were higher in the DCAT plans (P &lt; 0.001), with the most significant difference of 6.01% in the (2%, 1 mm) criteria. As for the interplay effect, the mean dose difference (MDD) in the DCAT plans was as good as the 3DCRT plans at different respiratory amplitudes but better than the IMRT plans (all P &lt; 0.05), and the MDD of DCAT plans did not exceed 3% in all respiratory amplitude.ConclusionIn centers lacking the VMAT technique, implementing SBRT treatment based on the DCAT technique for inoperable early-stage centrally-located NSCLC patients with PTV volumes &lt; 65 cc achieves better treatment efficiency and delivery accuracy while maintaining the plan quality.
Impact on parasitemia, survival time and pro-inflammatory immune response in mice infected with Plasmodium berghei treated with Eleutherine plicata
Authors: Quadros Gomes Antônio Rafael , Castro Ana Laura Gadelha , Ferreira Gleison Gonçalves , Brígido Heliton Patrick Cordovil , Varela Everton Luiz Pompeu , Vale Valdicley Vieira , Carneiro Liliane Almeida , Dolabela Maria Fâni , Percario Sandro 
Published: December 2024

In vitro studies with Plasmodium falciparum have demonstrated the antiparasitic activity of E. plicata, attributed to its naphthoquinones. This study reports on pro-inflammatory changes in mice infected with P. berghei and correlates these changes with parasitemia and survival. The ethanol extract of Eleutherine plicata (EEEp) was fractionated under reflux to obtain the dichloromethane fraction (FDMEp) and isolated compounds from E. plicata, relating these to survival time and parasitemia. Antimalarial activity was evaluated using the Peters suppressive test, with mice infected with Plasmodium berghei and treated with E. plicata, assessing parasitemia and survival over 30 days. The pro-inflammatory profile was determined by measuring interleukin-10, interferon-γ (IFN-γ), and nitric oxide levels. EEEp, FDMEp, and eleutherol showed activity on the 5th day of infection, with only FDMEp being active on the 8th day. Treatment with EEEp and FDMEp extended animal survival, reduced IFN-γ and NO levels, and increased IL-10 levels. Eleutherol significantly altered the response, with eleutherol glucuronide seemingly active by binding to lactate dehydrogenase, inhibiting hemozoin metabolism, leading to parasite death. Pro-inflammatory changes did not appear to correlate with survival and reduced parasitemia. In summary, FDMEp and eleutherol reduced parasitemia, extended survival, and modulated the inflammatory response. FDMEp and eleutherol are promising candidates for developing new antimalarial drugs.
Combination of traditional Chinese bone setting and NMES technique for treating lumbar disc herniation: a case report
Authors: Bao Yimei , Wang ZhiJin 
Published: December 2024

ObjectiveTo analyze and study the causes and treatment approaches for lumbar disc herniation, focusing on office workers.MethodsThe concept of spinal internal balance disorder as a foundation for treating traumatic spinal diseases was introduced. Pathological changes occurring with single (or multiple) vertebral displacement were considered. For the first time, the “spinal (point) rotation repositioning method” from traditional Chinese bone setting was combined with NMES (neuromuscular electrical stimulation) technology to treat low back pain.ResultsThe patient's symptoms of low back pain were cured within a short period, allowing a quick return to work and daily life, with no recurrence of the disease observed during long-term follow-up.ConclusionThe combination of traditional Chinese bone setting and NMES provides a more effective and quicker resolution to low back pain in office workers with lumbar disc herniation. This integrated approach not only relieves pain but also restores lumbar function, ensuring long-term stability and reducing recurrence.
Single-incision laparoscopic cholecystectomy reduced postoperative pain than three-incision laparoscopic cholecystectomy in patients with large gallstone, a retrospective study
Authors: Zhang Zhiheng , Xu Jiawei , Yu Decai , Lin Nacheng , Peng Jin 
Published: December 2024

AimTo compare the short-term outcomes between SILC and TILC depending on gallstone size.Material and methodsData from 114 patients with gallstones who underwent cholecystectomy hospitalized in Nanjing Drum Tower Hospital between June 2022 and October 2023 were collected. The gallstone diameter, the operation time, estimated blood loss, post-operative pain, complications post-operation, and length of hospital stay were all collected and examined.ResultsOf the 114 patients included in this study, 61 underwent SILC, and 53 underwent TILC. The pain score 6 h, 24 h post-operation was higher in the TILC group compared with the SILC group. Patients were divided into large (diameter &gt; 2 cm) and small groups (diameter &lt; 2 cm), larger gallstones significantly increased operation duration in the SILC group. For the TILC group, large gallstones significantly increased blood loss during the operation. The blood loss and pain scores were higher in the TILC group compared with the SILC group for patients with large gallstones.ConclusionIn this study, SILC and TILC both had comparable postoperative outcomes, while SILC significantly reduced postoperative pain than TILC. Moreover, SILC might be a suitable option for patients with larger gallstones (diameter &gt; 2 cm) and helps reduce blood loss and postoperative pain.
First metagenomic sequencing for the analysis of microbial community populations of adults and pupae of Melophagus ovinus in Xinjiang, China
Authors: Huang Kaijun , Zhang Xing , Xiong Na , Sun Lu , Zhao Xiaoqing , Zhou Kun , Wu Junyuan 
Published: December 2024

IntroductionMelophagus ovinus, a parasite on the body surface of sheep, directly attacks the host through biting and sucking blood and may also transmit pathogens in the process. There are currently only a few studies on the microbial composition of M. ovinus, while there are no such studies on pupae.MethodsIn this study, samples AT-1 to AT-4 each contained four M. ovinus individuals, while sample AT-5 comprised four M. ovinus pupae, all used for metagenomic sequencing and analysis. Melophagus ovinus adults and pupae were collected from four regions in Xinjiang, China. DNA was extracted from the samples, amplified, and sequenced using the Illumina Novaseq 6000 System; finally, the sequencing data were analyzed using molecular biology software.Results and discussionFrom all samples, a total of 32 phyla, comprising 372 genera and 1,037 species, were detected. The highest microbial diversity was observed in Kuqa City (AT-2) and Qira County (AT-4). Pupae exhibited 40 unique microbial genera (AT-5) but did not have the highest microbial diversity. Proteobacteria was the dominant phylum in all samples. The dominant genera included Bartonella, Wolbachia, Pseudomonas, and Arsenophonus. This is the first study to report most of the bacteria (e.g., Pseudomonas versuta and Arsenophonus nasoniae), fungi (e.g., Saitoella complicata), viruses (e.g., Orf virus and Wolbachia phage WO), and protozoa (e.g., Trypanosoma theileri and Babesia bigemina) in M. ovinus. This study has enriched the microbial diversity data of M. ovinus, and the pathogens it carries may pose a threat to public health safety and the economy of related industries, necessitating further research to develop effective biological control strategies.
Prostaglandin pathways in equine myometrium regulations: endometrosis progression
Authors: Piotrowska-Tomala Katarzyna K. , Szóstek-Mioduchowska Anna Z. , Drzewiecka Ewa M. , Jonczyk Agnieszka W. , Wójtowicz Anna , Wrobel Michał H. , Ferreira-Dias Graca , Skarzynski Dariusz J. 
Published: December 2024

IntroductionProstaglandins (PG) are important regulators of the myometrial contractility in mammals. Endometrosis, a condition characterized by morphological changes in the equine endometrium, also affects endometrial secretory function. However, it remains unclear whether and how endometrosis affects myometrial function.MethodsThis study investigated: (i) mRNA transcription of genes encoding specific enzymes responsible for PG synthesis, such as prostaglandin—endoperoxide synthase (PTGS2), PGE2 synthase (PTGES), PGF2α synthase (PTGFS) and PG receptors: PGE2 receptors (PTGER1- 4), and PGF2α receptor (PTGFS) in equine myometrium and, (ii) the effects of PGE2 and PGF2α on myometrial contractile activity, during endometrosis in mares. The myometria used in experiments 1 and 2 were collected from mares in the mid-luteal (n = 23) and follicular (n = 20) phases of the estrous cycle, according to the histological classification of the endometrium (Kenney and Doig categories I, IIA, IIB, and III).ResultsIn experiment 1, changes in mRNA transcription of PG synthase or PG receptors in the myometrium during the course of endometrosis were determined using qPCR. During the mid-luteal phase, myometrial mRNA transcription of PTGES increased in mares with endometrial category IIB compared to category I. However, myometrial mRNA transcription of PTGER1 decreased during the progression of endometrosis compared to category I. During the follicular phase, mRNA transcription of PTGER1 and PTGER2 increased in mares with endometrial categories III or IIA, respectively. In addition, mRNA transcription of PTGFS increased in mares with endometrium category IIA compared to category I. In experiment 2, the force of myometrial contractions was measured using an isometric concentration transducer. In the follicular phase, PGE2 decreased the force of contractions in mares with endometrial categories IIA, IIB, and III compared to the respective control groups. Prostaglandin F2α increased the force of myometrial contractions in mares with category IIA endometrium, whereas it decreased in category IIB compared to the respective control groups.DiscussionWe concluded that in the progression of endometrosis there are changes in the myometrial transcription of mRNA encoding PG synthases and receptors, particularly PTGER1 and PTGER2. Mares with endometrosis had abnormal myometrial contractile responses to PG. These findings suggest that myometrial function may be compromised during the progression of endometrosis.
Phenolic Constituents from Hypericum aucheri Jaub et. Spach—Isolation, Identification, and Preliminary Evaluation for hMAO-A/B and Neuroprotective Activity
Authors: Marinov Teodor , Kondeva-Burdina Magdalena , Kokanova-Nedialkova Zlatina , Nedialkov Paraskev T. 
Published: December 2024

Three new acylated benzophenone O-glycosides named aucherosides A–C (4–6), together with five known compounds such as mangiferin (1), maclurin-6-O-β-D-glucopyranoside (2), 1-O-galloyl-β-D-glucose (3), vanillic acid (7), and 5-hydroxy-2-isopropylchromone-7-O-β-glucoside (8), were isolated from the aerial parts of Hypericum aucheri Jaub. and Spach and identified with spectroscopic methods (1D and 2D NMR, and HRESIMS). Compounds 2, 4–6, 8, and previously isolated from the title plant aucherines A–C (9–11), were tested for hMAO-A and B inhibitory effects and neuroprotection. All tested compounds (1 µM) did not exhibit any inhibitory effect on hMAO-A and showed significant inhibitory activity against the hMAO-B enzyme. Notably, compound 8 demonstrated the strongest hMAO-B inhibition, approaching that of the positive control selegiline. At high concentrations (100 µM), all tested compounds showed no neurotoxic or pro-oxidant effects on rat brain synaptosomes, mitochondria, and microsomes. All tested compounds exhibited good neuroprotective and antioxidant activities in various neurotoxicity models (6-hydroxydopamine-induced neurotoxicity on synaptosomes, tert-butyl hydroperoxide-induced oxidative stress on mitochondria, and non-enzymatic lipid peroxidation on microsomes). The neuroprotective mechanisms of these compounds may include MAO-B inhibition, reactive oxygen species (ROS) scavenging, membrane stabilization, and preservation of reduced glutathione (GSH), the primary nucleophilic ROS scavenger.
68Ga-DOTATOC Uptake by Stellate Ganglia, Mimicking a Right Cervical Metastasis of Neuroendocrine Tumors: A Case Report
Authors: Zhang-Yin Jules Tianyu , Panagiotidis Emmanouil 
Published: December 2024

Background: 68Ga-DOTATOC PET/CT is a functional imaging modality that has revolutionized the evaluation of well-differentiated neuroendocrine tumors (NETs) by targeting somatostatin receptors. This technique has largely replaced conventional gamma camera imaging with 111In-labeled octreotide due to its superior sensitivity and resolution. While the physiologic distribution, normal variations, and common pitfalls associated with 68Ga-DOTATOC imaging are well documented, rare but clinically significant pitfalls can still occur. Methods: We present a case highlighting one such pitfall: focal 68Ga-DOTATOC uptake at the cervicothoracic junction, specifically within the stellate ganglia, which mimicked metastatic involvement of a NET. Results: Initially, the uptake was interpreted as a potential right cervical metastasis. To clarify this finding, a follow-up 68Ga-DOTATOC PET/CT was performed, which demonstrated no evidence of cervical metastases, thereby confirming the initial uptake as a physiologic variation rather than pathological activity. This case underscores the dynamic variability of 68Ga-DOTATOC uptake within the stellate ganglia in the same patient over time. On occasion, the intensity of physiologic uptake in these structures can be pronounced enough to mimic metastatic disease, posing a diagnostic challenge. Conclusions: Awareness of this rare phenomenon is essential to avoid misdiagnosis and unnecessary interventions.
Rectangular Improvement Method for Plan View Pattern of Plates During the Angular Rolling Process
Authors: He Chunyu , Luo Junyi , Xu Zhipeng , Wang Zhiqiang , Zhao Zhong , Wu Zhiqiang , Jiao Zhijie 
Published: December 2024

The effect of the angular rolling process on the plan view pattern of a plate was studied, and the rectangular influencing factors and improvement methods for this process were proposed in this paper. DEFORM (v11.0) finite element software was used to simulate the processes of conventional rolling and angular rolling, and the degree of rectangularity of plates under different rolling process conditions was compared. A formula to characterize the degree of rectangularity of plates was established; the closer this value is to one, the better the degree of rectangularity. Considering the actual rolling process conditions, the range of theoretically calculated rectangular rotation angles was extended to obtain the optimum rectangular rotation angle using the finite element simulation method. In the two-pass angular rolling process, the optimal rectangular angle of the second pass was 14.275° when the first pass was 15°. The optimal rectangular angle of the plate was 19.008° when the first pass’ angle was 20°. Two-pass angular rolling is different to four-pass rolling, and the simulation results showed that J 15° 4 (1.0012) was less than J 15° 2 (1.0015) and J 20° 4 (1.0034) was less than J 20° 2 (1.0055). The rectangularity degree of the four-pass process was better than the two-pass process. Angular rolling experiments were carried out, and the actual data show that the characteristic rectangular value of the rolled piece was 1.003 during the four-pass process and 1.014 during the two-pass process. This verified that separating the one-group two-pass angular rolling process from the one-group four-pass angular rolling process can improve the rectangular degree of the rolled plate, thereby increasing the yield rate. This provides a theoretical basis for industrial applications.
Micoses superficiais e cutâneas: estudo epidemiológico para identificar pessoas que realizam diagnóstico e tratamento adequado
Authors: Pereira Katlen Caroline Fausto , Oliveira Fernanda Sant Ana de Siqueira e , Brisoli Cleber 
Published: December 2024

As micoses superficiais e cutâneas são doenças causadas por fungos. Doenças comuns, de fácil disseminação que atingem milhões de pessoas no mundo todo. Por ser uma patologia não muito rara no dia a dia e rotina de diversas pessoas de diferentes classes sociais e faixa etária, e por, na maioria das vezes não causar malefícios à saúde, os afetados acabam se autodiagnosticando e se automedicando ao invés de procurar ajuda médica. Considerando o fato de que as dermatofitoses não são doenças consideradas de notificação obrigatória e não há grandes projetos que visam a importância de um diagnóstico e tratamento correto, essa pesquisa teve como objetivo realizar um levantamento de dados para que fosse feita uma análise, e identificar por meio de uma pesquisa entre as pessoas que já tiveram ou tem micoses superficiais e cutâneas quais tiveram diagnóstico e tratamento por um profissional capacitado e qualificado. O diagnóstico assertivo destas patologias pode ajudar, não somente em um prognóstico e tratamento correto das causas, mas também melhorar a qualidade de vida dos pacientes, que muitas vezes tentam camuflar esta doença para serem aceitos e, ou terem um melhor convívio na sociedade.
Stabilization of a silicon double quantum dot based on a multi-dimensional gradient descent technique
Authors: Wen Chutian , Takahashi Hiroki , Ibad Sayyid Irsyadul , Nishiyama Shimpei , Kato Kimihiko , Liu Yongxun , Murakami Shigenori , Mori Takahiro , Mizokuchi Raisei , Yoneda Jun , Kodera Tetsuo 
Published: December 2024

Abstract
With a view to long-term qubit device operation, we report on a method to stabilize a silicon double quantum dot against slow drift in a two-dimensional gate-voltage space based on a current gradient-based feedback technique. We demonstrate that, unlike conventional single-axis feedback schemes, our method can maintain the double-dot potential configuration. We measure a feedback bandwidth of up to 300 mHz, consistent with the sampling rate and the digital filter cutoff frequency used in the experiment.
PENGARUH EKSISTENSI BANK MUAMALAT INDONESIA DAN AKAD MUSYARAKAH TERHADAP KEPUTUSAN MASYARAKAT MENJADI NASABAH
Authors: Naim Muhammad 
Published: December 2024

Keberadaan Bank Muamalat Indonesia, Tbk Cabang Mataram adalah manjadi bank syariah utama di Indonesia, dominan dipasar spiritual, dikagumi dipasar rasional. Pada umumnya masalah yang dihadapi masyarakat kecil dalam mengembangkan usaha adalah modal usaha.
Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh eksistensi bank muamalat Indonesia dan akad musyarakah terhadap keputusan masyarakat menjadi nasabah dibank muamalat Indonesia,Tbk Cabang Mataram. Jenis penelitian ini adalah kuantitatif dengan menggunakan pendekatan asosiatif dengan tujuan untuk mengetahui hubungan dua variabel atau lebih. Pada penelitian ini, dalam menentukan sampel peneliti menggunakan probability sampling dengan metode Simple Random Sampling dengan jumlah populasi 187 orang yang menggunakan akad musyarakah dan jumlah sampel 83 orang dengan menggunakan rumus Slovin. Jenis data yang dikumpulkan dalam penelitian ini adalah data primer dan skunder. Tehnik pengumpulan data menggunakan kuesioner dan analisis data hasil penelitian dilakukan menggunakan metode kuantitatif dengan analisis data uji validitas dan reabilitas yang dibantu oleh aplikasi SPSS for windows dan menggunakan analisi regresi berganda dengan uji pengaruh secara parsial dan simultan terhadap variabel.
Hasil penelitian keberadaan Bank Muamalat Indonesia dan produk akad musyarakah dikota mataram sebagai variabel bebas berpengaruh secara parsial terhadap keputusan masyarakat mejadi nasabah. Selain itu juga, kedua variabel bebas secara simultan memiliki pengaruh signifikan terhadap variabel terikat. Disebabkan kenyamanan pelayanan bank, kemudahan syarat adminstrasi, dan penerapan prinsip bagi hasil sesuai dengan hukum Islam pada aktivitas perbankan juga memberikan kemudahan dan kenyamanan bagi masyarakat dalam mengembangkan dunia usahanya. Sehinggga bagi masyarakat tidak perlu meminjam uang kepada bank konvensional yang menggunakan sistem bunga yang sudah ditentukan oleh pihak bank tersebut.