bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long noncoding RNA lnc‐ABCA12‐3 promotes cell migration, invasion, and proliferation by regulating fibronectin 1 in esophageal squamous cell carcinoma
Authors: Ma Junliang , Xiao Yuhang , Tian Bo , Chen Shaolin , Zhang Baihua , Wu Jie , Wu Zhining , Li Xu , Tang Jinming , Yang Desong , Zhou Yong , Wang Hui , Su Min , Wang Wenxiang 
Published: September 2019

AbstractLong noncoding RNAs (lncRNAs) have been shown to play important roles in human cancers, including esophageal squamous cell carcinoma (ESCC). We previously demonstrated that a novel lncRNA, lnc‐ABCA12‐3, was overexpressed in ESCC tissues. However, the exact function of lnc‐ABCA12‐3 is unknown. In the current study, we aimed to evaluate the expression of lnc‐ABCA12‐3 in ESCC and to explore the potential mechanism of lnc‐ABCA12‐3 in cell migration, invasion, and proliferation. We showed that lnc‐ABCA12‐3 was upregulated in ESCC tumor tissues and cell lines. The increased expression of lnc‐ABCA12‐3 was positively associated with advanced tumor‐node‐metastasis stages and poor prognosis. The knockdown of lnc‐ABCA12‐3 inhibited the cell migration, invasion, and proliferation abilities of KYSE‐510 and Eca‐109 cells. We also found that fibronectin 1 (FN1) was upregulated in ESCC tumor tissues. The expression of FN1 messenger RNA was positively correlated with the expression of lnc‐ABCA12‐3 in ESCC tumor tissues. After lnc‐ABCA12‐3 knockdown, the expression of FN1 was downregulated. In addition, the overexpression of FN1 restored the abilities of cell migration, invasion and proliferation in Eca‐109 cells. Further studies indicated that lnc‐ABCA12‐3 acted as a competing endogenous RNA for miR‐200b‐3p to regulate FN1 expression. In conclusion, these results suggest that lnc‐ABCA12‐3 is a novel oncogene in tumorigenesis and that its high expression is related to a poor prognosis for patients with ESCC. lnc‐ABCA12‐3 promotes cell migration, invasion, and proliferation via the regulation of FN1 in ESCC. Our data suggest that lnc‐ABCA12‐3 might serve as a potential prognostic biomarker and therapeutic target for ESCC.
MicroRNA‐193a and taxol combination: A new strategy for treatment of colorectal cancer
Authors: Hejazi Maryam , Baghbani Elham , Amini Mohammad , Rezaei Tayebeh , Aghanejad Ayuob , Mosafer Jafar , Mokhtarzadeh Ahad , Baradaran Behzad 
Published: September 2019

AbstractMicro RNAs (miRNAs) show a considerable promise as a therapeutic agent for combination therapy of colorectal cancer (CRC). Given that, the current study was purposed to explore the potential therapeutic role and underlying mechanism of miR‐193a as a promising tumor suppressor in human CRC cell lines in combination with Taxol. Therefore, HT‐29 cells with the lowest expression levels of miR‐193a were treated with miR‐193a mimics and Taxol, separately or in combination. Functional analyses showed that the combination therapy inhibited migration and colony formation of HT‐29 cells and arrested the cell cycle at the G1 phase. Moreover, treatment with Taxol reduced cell survival with an increase in mRNA expression of metastasis‐related genes caspase‐3 and caspase‐9, whereas miR‐193a transfection alone didn't significantly influence cell viability and apoptosis induction. Quantitative reverse transcription polymerase chain reaction results also revealed that miR‐193a replacement decreased the expression levels of c‐Myc, MMP‐9, vimentin, and ROCK in treatment groups compared to the controls. Therefore, it could be concluded miR‐193a inactivates cell migration via suppression of metastasis pathways in CRC and through downregulation of c‐Myc, acts as a negative regulator of cell cycle and growth. Then, our findings imply that miR‐193a replacement combined with Taxol chemotherapy could be considered as a new potential therapeutic approach for improvement of CRC treatment.
Antithyroid cancer effects of human neural stem cells expressing therapeutic genes on anaplastic thyroid cancer cells
Authors: Shin Hye‐Ji , Hwang Kyung‐A. , Go Ryeo‐Eun , Kim Seung U. , Choi Kyung‐Chul 
Published: September 2019

AbstractStem cells that express therapeutic proteins have been identified to have an anticancer effects on various types of cancer. In the present study study, human neural stem cells (hNSCs) that were genetically engineered to express cytosine deaminase (CD) and human interferon‐β (IFN‐β) were used for anaplastic thyroid cancer (ATC) treatment owing to their tumor‐tropic properties and therapeutic effects. CD is an enzyme that converts 5‐fluorocytosine (5‐FC), a prodrug, to 5‐fluorouracil (5‐FU) which is a medication to suppress tumor growth through DNA synthesis inhibition. Also, IFN‐β suppresses tumor growth by the induction of apoptotic process. In water soluble tetrazolium salt (WST) assay, SNU‐80 cells which are human female ATC cells were cocultured with three cell types including engineered hNSCs such as HB1.F3, HB1.F3.CD, and HB1.F3.CD.IFN‐β cells on transwells and treated with 5‐FC for 72 hours. Finally, the SNU‐80 cell viability was reduced by the coculture with HB1.F3.CD and HB1.F3.CD.IFN‐β cells. In dichlorofluorescein diacetate (DCF‐DA) and TdT‐mediated dUTP nick‐end labeling (TUNEL) assays, the production of reactive oxygen species (ROS) and the number of apoptotic cells were increased by HB1.F3.CD and HB1.F3.CD.IFN‐β cells in the presence of 5‐FC. In Western blot assay, ROS, and apoptosis‐related genes were increased in SNU‐80 cells when they were cocultured with HB1.F3.CD and HB1.F3.CD.IFN‐β cells. In transwell migration assay, hNSCs selectively migrated to SNU‐80 cells because hNSCs interacted with chemoattractant factors like SDF‐1α, uPAR, and CCR2 secreted by SNU‐80 cells. Taken together, engineered hNSCs were revealed to selectively migrate to ATC cells and to inhibit growth as well as to induce apoptosis of ATC cells via ROS production through the actions of transgenes such as CD and IFN‐β. Therefore, these engineered hNSCs can be promising candidates for the treatment of metastatic ATC.
Characterization and optimization of immunoaffinity aqueous two‐phase systems with PEGylated CD133/2‐biotin antibody in route to stem cell separation
Authors: Ornelas‐González Alonso , Reisenauer Stefanie U , González‐González Mirna , Rito‐Palomares Marco 
Published: September 2019

AbstractBACKGROUNDA short processing time and efficient scale‐up stem cell isolation bioprocess is essential to exploit the potential of these cells for the treatment of multiple chronic diseases. Various methodologies have been used for stem cell recovery. However, most of them present economic and/or time‐consuming drawbacks.RESULTSThis paper reports the characterization and optimization of immunoaffinity aqueous two‐phase systems (ATPS), a liquid‐liquid based separation technology enhanced with the PEGylation of the antibody as a promising platform in route to the separation of CD133+ stem cells. The best results were obtained with Ucon‐DEX or PEG‐DEX systems with tie‐line length (TLL) 15 or 20% w/w and volume ratio (VR) 3. When the PEGylated antibodies were added to these selected optimized ATPS, they partitioned to both phases, but with higher preference for the bottom phase.CONCLUSIONPEGylation was an effective strategy to induce a change in the partition behavior of the CD133/2‐biotin antibody when Ucon‐DEX ATPS were used. Similarly, variation of the parameters (TLL and VR) resulted to be useful for this goal. Nevertheless, further studies are required to find the optimal ATPS composition that will fractionate 100% of the antibody to the contaminants' opposite phase, making this system an ideal candidate to be tested for the selectivity of CD133+ stem cells. © 2019 Society of Chemical Industry
An effective green and ecofriendly catalyst for synthesis of bis(indolyl)methanes as promising antimicrobial agents
Authors: Nemallapudi Bakthavatchala Reddy , Zyryanov Grigory V. , Avula Balakrishna , Guda Mallikarjuna Reddy , Gundala Sravya 
Published: September 2019

AbstractAn effective and suitable meglumine‐catalyzed high‐yielding process was considered and engaged for the synthesis of new bis(indolyl)methanes at ambient temperature under aqueous conditions. The catalytic reaction proceeds very smoothly. Clean reaction, ease of product isolation/purification, easily available reactant and ecofriendly reaction conditions are the notable advantages of the present methodology. All the title compounds were characterized by IR, 1H, 13C NMR, and mass spectra. All the synthesized compounds were tested for antimicrobial activity, and the results indicated that most of the synthesized compounds exhibited excellent activity against the tested microorganisms. In this new series, compound 3l, having nitro substituent on the aromatic ring, showed exceptional potent inhibitory activity against Pseudomonas aeruginosa and Penicillium chrysogenum.
Design, synthesis, and anticancer activities of sodium quinazolin‐4‐diselenide compounds
Authors: Zhang Yuchun , Niu Pengpeng , Wen Quanwu , Sun Lin , Wang Weili , Xu Shengguang , Liu Gang 
Published: September 2019

AbstractSubstituted 4‐chloroquinazoline reacted with sodium diselenide to give novel sodium quinazoline‐4‐diselenide compounds. The reaction provides an efficient and facile approach to the synthesis of sodium quinazoline‐4‐diselenide compounds. Structures of title compounds were confirmed by IR, 1H NMR, 13C NMR, and elemental analysis. MTT assay was adopted to show anticancer activities of the compounds. Compounds 5a and 5h showed good activities against cancer‐cell lines MDA‐MB‐435, MDA‐MB‐231, A549, SiHa, and HeLa. In addition, 5a exhibited quite good anticancer effects on relative above cell lines with 10μM concentration compared with oxaliplatin and gefitinib of the commercial anticancer drugs.
Dry versus wet and gross: Comparisons between the dry skull method and gross dissection in estimations of jaw muscle cross‐sectional area and bite forces in sea otters
Authors: Law Chris J. , Mehta Rita S. 
Published: September 2019

AbstractBite force is a measure of feeding performance used to elucidate links between animal morphology, ecology, and fitness. Obtaining live individuals for in vivo bite‐force measurements or freshly deceased specimens for bite force modeling is challenging for many species. Thomason's dry skull method for mammals relies solely on osteological specimens and, therefore, presents an advantageous approach that enables researchers to estimate and compare bite forces across extant and even extinct species. However, how accurately the dry skull method estimates physiological cross‐sectional area (PCSA) of the jaw adductor muscles and theoretical bite force has rarely been tested. Here, we use an ontogenetic series of southern sea otters (Enhydra lutris nereis) to test the hypothesis that skeletomuscular traits estimated from the dry skull method accurately predicts test traits derived from dissection‐based biomechanical modeling. Although variables from these two methods exhibited strong positive relationships across ontogeny, we found that the dry skull method overestimates PCSA of the masseter and underestimates PCSA of the temporalis. Jaw adductor in‐levers for both jaw muscles and overall bite force are overestimated. Surprisingly, we reveal that sexual dimorphism in craniomandibular shape affects temporalis PCSA estimations; the dry skull method predicted female temporalis PCSA well but underestimates male temporalis PCSA across ontogeny. These results highlight the importance of accounting for sexual dimorphism and other intraspecific variation when using the dry skull method. Together, we found the dry skull method provides an underestimation of bite force over ontogeny and that the underlying anatomical components driving bite force may be misrepresented.
Circulating miRNA expression profile and bioinformatics analysis in patients with occult hepatitis B virus infection
Authors: Hao Qing‐qin , Wang Qing‐hui , Xia Wei , Qian Hui‐zhong 
Published: September 2019

AbstractEmerging suggest that microRNAs (miRNAs) play vital roles in the occurrence and development of hepatitis B virus (HBV) infectious disease. However, miRNAs in occult hepatitis B virus infection (OBI), a special stage of HBV infection, remain largely unknown. Herein, we conducted this study to identify differentially expressed miRNAs and then to investigate the potential roles of these miRNAs in OBI. Plasma miRNA expression profiles of three OBI patients and three healthy controls were analyzed with high through‐put miRNA sequencing technology. Altered expression of miRNAs was further confirmed with reverse transcription quantitative polymerase chain reaction (qRT‐PCR). Finally, bioinformatics analysis was conducted to investigate the involved pathways and target genes for these differentially expressed miRNAs. Totally, 32 differentially expressed miRNAs were identified between OBI and healthy controls by miRNA sequencing (fold change ≥ 1.5, P < .1, and counts per million reads ≥ 1), including 16 downregulated and 16 upregulated miRNAs. Differential expression of hsa‐miR‐486‐5p, ‐25‐3p, and ‐92a‐3p and ‐1‐3p was further validated by qRT‐PCR analysis, which was consistent with miRNA sequencing analysis. Moreover, these four miRNAs might distinguish OBI from HCs efficiently. Bioinformatics analyses indicated that the differentially expressed miRNAs were primarily involved in various biological processes related to gene expression and transcription, cell development and metabolism, protein modification and kinase activity regulation, as well as multiple signaling pathways such as PI3K/Akt signaling pathway. This study provided a global view of miRNA expression in plasma from OBI patients. These differentially expressed miRNAs might play important roles in the development of OBI, which provided intriguing insights into the screening and molecular mechanism of OBI.
The role of the Atlantic Multidecadal Oscillation precondition in the teleconnection of different El Niño‐Southern Oscillation types and impacts on the 15°N–15°S South American sector precipitation
Authors: Figliuolo Guilherme C. , Andreoli Rita V. , Kayano Mary T. , da Costa Jean Antunes Custódio , Rego Willy H. T. , de Moraes Djanir Sales 
Published: September 2019

AbstractThe Eastern Pacific (EP) and Central Pacific (CP) El Niño‐Southern Oscillation (ENSO) types and their impacts on the tropical North Atlantic (TNA) SST variability and 15°N–15°S South American precipitation during the warm and cold phases of the Atlantic Multidecadal Oscillation (WAMO and CAMO) were evaluated during the 1901–2012 period. The results show more frequent ENSO events during the CAMO. The El Niño (EN) (La Niña [LN]) events, regardless of type (EP or CP), during the WAMO (CAMO) were accompanied by a warming (cooling) in the TNA after its mature phases. In these cases, extratropical teleconnection patterns are established through variations in the Pacific/North America (PNA) teleconnection pattern and are accompanied by variations in the Walker circulation. For the EN (LN) in the CAMO (WAMO), the tropical teleconnections occur predominant, through the Walker cell and the zonal inter‐basin gradient, which is intensified due to the SST gradient between the eastern equatorial Pacific (non‐neutral anomalies) and the equatorial Atlantic (neutral anomalies). These circulation pattern changes affect the precipitation patterns in the 15°N–15°S South American sector during December–January–February (D(0)JF(+1)) and March–April–May (MAM(+1)). The EP EN (EPEN) events are associated with the intensification of the negative precipitation anomalies in northeastern Brazil (NEB) during the WAMO and in the central part of the Amazon during the CAMO. In the case of CP EN (CPEN) events, the greatest differences between the AMO phases occur during MAM(+1), with reverse sign anomalies over northwestern South America. In the case of LN events, the largest differences occur in NEB, with reduced rainfall in the WAMO, regardless of type EP or CP. The results presented here highlight the role of low frequency oscillations in defining the teleconnection patterns between tropical Pacific and Atlantic Oceans, not discussed previously.
Northwestwards shift of tropical cyclone genesis position during autumn over the western North Pacific after the late 1990s
Authors: Cao Xi , Liu Yong , Wu Renguang , Bi Mingyu , Dai Yifeng , Cai Zelin 
Published: September 2019

AbstractPrevious study has suggested a northwestwards shift of tropical cyclone (TC) genesis position during autumn over the western North Pacific (WNP) after the late 1990s. This study replenishes two mechanisms to explain this inter‐decadal change in the WNP TC genesis position. The first mechanism is related to the mean state change. According to observational analysis and numerical simulations with the Geophysical Fluid Dynamics Laboratory (GFDL) atmospheric general circulation model, a La Niña‐type Pacific sea surface temperature (SST) change and the northern Atlantic Ocean warming in the late 1990s cause a dipole pattern of lower‐level circulation change, with cyclone over the northwestern WNP and anticyclone over the southeastern WNP. This pattern is favourable for more TC genesis in the northwestern WNP. The sensitivity experiments show that the contribution from the Pacific Ocean is larger than that from the northern Atlantic Ocean. The second mechanism is related to the change in the intra‐seasonal oscillation intensity, particularly the quasi‐biweekly variation. The activity of intra‐seasonal oscillations over the southeastern WNP shows a decadal decrease around the late 1990s. Meantime, when the intra‐seasonal oscillations with tilted structure move northwestwards, they provide a favourable condition for TC genesis to be located more northwestwards after the late 1990s.
Malnutrition Predisposes to Endotoxin‐Induced Edema and Impaired Inflammatory Response in Parenterally Fed Piglets
Authors: Bæk Ole , Fabiansen Christian , Friis Henrik , Ritz Christian , Koch Jørgen , Willesen Jakob L. , Heegaard Peter M. H. , Lykkesfeldt Jens , Briend André , Golden Michael H. , Sangild Per T. , Thymann Thomas 
Published: September 2019

AbstractBackground: Poor nutrition status is common among hospitalized children and children in low‐income countries and may be associated with increased susceptibility to edema and infections. We hypothesized that poor nutrition status, established with a suboptimal composition of parenteral nutrition (PN), predisposes to endotoxemia‐induced edema, oxidative stress, and dysregulated immune responses. Methods: Using a 2 × 2 factorial design, 3‐day‐old piglets (n = 40) were given either optimal or suboptimal composition of PN for 7 days and then infused with either saline or lipopolysaccharide (LPS) for 9 hours to induce an acute‐phase reaction. Abdominal tissue edema and blood markers of immunity, inflammation, and oxidative stress were assessed. Results: Piglets receiving suboptimal nutrition showed signs of malnutrition with restricted growth, signs of inflammation (elevated C‐reactive protein [CRP], interleukin‐6, and serum amyloid A levels), oxidative stress (lower erythrocyte glutathione/hemoglobin and α‐tocopherol/cholesterol ratios), and liver dysfunction (increased liver weight and blood bilirubin levels). Perirenal edema was more excessive in malnourished LPS‐infused animals, relative to healthy LPS‐infused control animals (P < .01). Malnutrition reduced the inflammatory response to LPS (lower CRP, tumor necrosis factor‐α, haptoglobin, and neutrophil to lymphocyte ratio) but did not influence LPS‐induced oxidative stress markers. Conclusions: We conclude that endotoxemia and malnutrition in combination lead to acute‐phase hyporesponsiveness and perirenal edema in piglets. This finding may have implications for pediatric patients that suffer from malnutrition, as their response to bacterial infections may differ substantially from patients of normal nutrition status.
The association between gingival recession and buccal bone at maxillary anterior teeth
Authors: D'Silva Eric , Fraser David , Wang Bokai , Barmak Abdul Basir , Caton Jack , Tsigarida Alexandra 
Published: September 2019

AbstractBackgroundGingival recession and a thin or absent buccal plate occur frequently at maxillary anterior teeth and necessitate careful treatment planning to prevent future complications. However, the association between these two conditions is unclear and the ability of gingival recession to predict underlying buccal bone deficiencies is unknown. Therefore, the aim of this study is to use clinical and radiographic data to test this association and determine the influence of demographic and clinical parameters on both conditions.MethodsThis investigation comprised a single‐center, retrospective study. Data were derived from periodontal examinations performed on 66 adult subjects. Corresponding cone‐beam computed tomography images were used to measure the width of buccal bone at two points along the root surface and the distance between the bone crest and cemento‐enamel junction (CEJ). Results were then analyzed to determine the association between the presence of gingival recession and the condition of radiographic buccal bone, as well as the relative contribution of demographic parameters and other clinical findings to gingival recession and buccal bone conditions.ResultsGingival recession was present at 32.9% of maxillary anterior teeth and was most common at canines, followed by lateral incisors and central incisors. Mean buccal bone widths were significantly less, and the distance between the CEJ and bone crest was significantly greater for teeth with recession. Accordingly, gingival recession was a significant predictor for buccal bone thickness <1 mm at the level of 4 mm apical to the CEJ (odds ratio 2.733, 95% confidence interval 1.644 to 4.543, P < 0.0001). Probing depths were related to the presence or absence of gingival recession, while patient sex, age, and the apico‐coronal height of the gingiva were related to buccal bone thickness.ConclusionWithin the limitations of this study, maxillary anterior teeth with pre‐existing gingival recession were more likely to have thin (<1 mm) buccal bone.
Comparison of initial warfarin dosing in hospitalised patients considered sensitive to warfarin: low‐dose versus standard‐dose strategy
Authors: Alamri Abdulrahman M. , Almogbel Yasser S. , Salazar Miguel , Putney Kimberly , Bayat Maryam 
Published: September 2019

AbstractWarfarin dosages are individualised based on disease state, target International Normalised Ratio (INR) and potential risk factors for bleeding. This retrospective case series study compared and evaluated the safety and time to reach target INR (therapeutic INR and INR >3) of two warfarin dose initiation strategies (2.5 vs 5–7.5 mg; Groups 1 and 2, respectively) in potentially warfarin‐sensitive patients. In addition, the incidence of bleeding within 15 days of starting warfarin therapy was determined. In in all, 137 patients were included in the study. The mean (±SD) time to reach an INR ≥2.0 was 7.4 ± 2.3 vs 5.84 ± 1.82 days in Groups 1 and 2, respectively. There was no significant difference between groups in patients attaining INR >3 (p = 0.1487). In conclusion, some patients considered sensitive to warfarin could receive a loading dose of 5–7.5 mg and attain therapeutic INR faster than patients receiving 2.5 mg without an increased risk of bleeding.