bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of lipopolysaccharide and juvenile hormone III treatments on cell growth and gene expression in the Ceratitis capitata (Diptera: Tephritidae) CCE/CC128 cell line
Authors: García‐Reina Andrés , Rossi Elisabetta , Galián José 
Published: September 2019

AbstractThe Mediterranean fruit fly Ceratitis capitata is one of the most important insect pest species in the world. It has a high colonization capacity and population variety, giving it considerable genetic diversity. Strategies for its control have included the sterile insect technique and insect growth regulators. Many studies have analyzed the medfly transcriptome, and along with the medfly genome sequence, the sequences of multiple genes related to sex determination, mating, development, pheromone detection, immunity, or stress have been identified. In this study, the medfly CCE/CC128 cell line was used to assess cell growth variation and changes in the expression of genes covering different functions, after lipopolysaccharide (LPS) and juvenile hormone III (JHIII) treatments. No significant effects on cell growth and gene expression were observed in the cells treated with LPS. In the cells treated with JHIII, the results showed significant effects on cell growth, and an overexpression was found of the Shade gene, one of the Halloween gene members of the cytochrome p450 family, which is involved in development and the synthesis of 20‐hydroxyecdysone. This study shows preliminary results on the insect cell line in combination with whole‐genome sequencing, which can facilitate studies regarding growth, toxicity, immunity, and transcriptome regulations as a response to different compounds and environmental alterations.
Benefit of Anakinra in Treating Pediatric Secondary Hemophagocytic Lymphohistiocytosis
Authors: Eloseily Esraa M. , Weiser Peter , Crayne Courtney B. , Haines Hilary , Mannion Melissa L. , Stoll Matthew L. , Beukelman Timothy , Atkinson T. Prescott , Cron Randy Q. 
Published: September 2019

ObjectiveTo assess the benefit of the recombinant human interleukin‐1 receptor antagonist anakinra in treating pediatric patients with secondary hemophagocytic lymphohistiocytosis (HLH)/macrophage activation syndrome (MAS) associated with rheumatic and nonrheumatic conditions.MethodsA retrospective chart review of all anakinra‐treated patients with secondary HLH/MAS was performed at Children's of Alabama from January 2008 through December 2016. Demographic, clinical, laboratory, and genetic characteristics, outcomes data, and information on concurrent treatments were collected from the records and analyzed using appropriate univariate statistical approaches to assess changes following treatment and associations between patient variables and outcomes.ResultsForty‐four patients with secondary HLH/MAS being treated with anakinra were identified in the electronic medical records. The median duration of hospitalization was 15 days. The mean pretreatment serum ferritin level was 33,316 ng/ml and dropped to 14,435 ng/ml (57% decrease) within 15 days of the start of anakinra treatment. The overall mortality rate in the cohort was 27%. Earlier initiation of anakinra (within 5 days of hospitalization) was associated with reduced mortality (P = 0.046), whereas thrombocytopenia (platelet count <100,000/μl) and STXBP2 mutations were both associated with increased mortality (P = 0.008 and P = 0.012, respectively). In considering patients according to their underlying diagnosis, those with systemic juvenile idiopathic arthritis (JIA) had the lowest mortality rate, with no deaths among the 13 systemic JIA patients included in the study (P = 0.006). In contrast, those with an underlying hematologic malignancy had the highest mortality rate, at 100% (n = 3).ConclusionThese findings suggest that anakinra appears to be effective in treating pediatric patients with non–malignancy‐associated secondary HLH/MAS, especially when it is given early in the disease course and when administered to patients who have an underlying rheumatic disease.
Microscopic and spectroscopic study of the corona formation and unfolding of human haemoglobin in presence of ZnO nanoparticles
Authors: Bhunia A.K. , Saha S. , Kamilya T. 
Published: September 2019

AbstractThe interaction of zinc oxide nanoparticles (ZnO NPs) with human haemoglobin (Hb) is studied for the biologically safe application of ZnO NPs in the human body. The Hb corona is formed around the ZnO nanoparticles, directly observed from high‐resolution transmission electron microscopy (HRTEM) images. Hb formed ‘hard corona' on the surface of ZnO NPs from an exponential association mechanism over a very short duration, as well as unfolding of Hb that occurred over a long lifetime. Dynamic light scattering measurements demonstrated that the ZnO NPs were completely covered by Hb with shell thickness of c. 6 nm that formed a ‘hard corona'. Zeta potential measurements represented that the ZnO NPs were fully covered by Hb molecules using an exponential association mechanism. Tryptophans (TRY), as well as heme‐porphyrin moieties of Hb, are the major binding sites for ZnO NPs. The nature of the interaction between ZnO NPs and Hb was analysed from the fluorescence quenching of TRYs. Electrostatic interaction, along with the hydrophobic interaction between ZnO NPs and Hb, is responsible for the conformational change in Hb due to increase in the percentage of β‐sheets together with a decrease in α‐helices.
Pseudobactins bounded iron nanoparticles for control of an antibiotic‐resistant Pseudomonas aeruginosa ryn32
Authors: Kotb Essam , Ahmed Asmaa A. , Saleh Tawfik A. , Ajeebi Afnan M. , Al‐Gharsan Mona S. , Aldahmash Noura F. 
Published: September 2019

AbstractAmong 50 strains of Pseudomonas aeruginosa tested for the resistance to antibiotics, strain ryn32 was selected for this study based on its resistance level. It showed complete resistance toward aztreonam and almost complete resistance (96%) against kanamycin. Iron nanoparticles (FeNPs) were then prepared and found with diameters 30–50 nm. The threshold level of FeNPs for pyoverdines (PVDs) production by P. aeruginosa ryn32 was found at 25 μM concentration. PVDs production was optimal with pH 7.5, 35°C, succinate as carbon source, ammonium sulfate as nitrogen source at 60 hr fermentation time. Interestingly, when used the PVDs as conjugates with FeNPs they showed antibacterial action against the producing strain and some other gram‐negative bacteria. This suggests that the conjugates enter the bacterial cell via the ferriPVDs uptake pathway, which triggers the accumulation of FeNPs inside the cell, which is crucial on bacterial viability. Growth stimulation with the same concentrations of FeNPs and PVDs in separate treatments supported this view.
Process characterization strategy for a precipitation step for host cell protein reduction
Authors: Prentice Jessica , Vu Diemchi , Robbins David , Ferreira Gisela 
Published: September 2019

AbstractProcess characterization using QbD approaches has rarely been described for precipitation steps used for impurity removal in biopharmaceutical processes. We propose a two‐step approach for process characterization in which the first step focuses on product quality and the second focuses on process performance. This approach provides an efficient, streamlined strategy for the characterization of precipitation steps under the Quality by Design paradigm. This strategy is demonstrated by a case study for the characterization of a precipitation using sodium caprylate to reduce host cell proteins (HCP) during a monoclonal antibody purification process. Process parameters were methodically selected through a risk assessment based on prior development data and scientific knowledge described in the literature. The characterization studies used two multivariate blocks to decouple and distinguish the impact of product quality (e.g., measured HCP of the recovered product from the precipitation) and process performance (e.g., step yield). Robustness of the precipitation step was further demonstrated through linkage studies across the overall purification process. HCP levels could be robustly reduced to ≤100 ppm in the drug substance when the precipitation step operated within an operation space of ≤1% (m/v) sodium caprylate, pH 5.0–6.0, and filter flux ≤300 L/m2‐hr for a load HCP concentration up to 19,000 ppm. This two‐step approach for characterization of precipitation steps has several advantages, including tailoring of the experimental design and scale‐down model to the intended purpose for each step, use of a manageable number of experiments without compromising scientific understanding, and limited time and material consumption.
Bioprocess strategies for enhancing biomolecules productivity in Chlorella fusca LEB 111 using CO2 a carbon source
Authors: Moraes Luiza , Santos Lucielen O. , Costa Jorge A. V. 
Published: September 2019

AbstractThe aim of this study was to evaluate the influence of different carbon dioxide (CO2) concentrations on the distribution of carbon forms in the culture medium and the biomass production and biomolecules productivity of the strain Chlorella fusca LEB 111. In this study, experiments were carried out in which C. fusca cultures were exposed to different CO2 concentrations, 0.03% (0.08 mlCO2 mlmedium−1 days−1), 5% (0.18 mlCO2 mlmedium−1 days−1), and 15% vol/vol CO2 (0.54 mlCO2 mlmedium−1 days−1). Among the carbon chemical species distributions in the culture medium, bicarbonate was predominant (94.2–98.9%), with the highest quantitative percentage in the experiment receiving a 15% CO2 injection. C. fusca LEB 111 cultivated with 15% CO2 showed the highest biomass productivity (194.3 mg L−1 days−1) and CO2 fixation rate (390.9 mg L−1 days−1). The carbohydrate productivity in the culture that received 15% CO2 was 46.2% higher than the value verified for the culture with the addition of CO2 from the air (0.03% CO2). In addition, CO2 concentration providing increases of 0.03–15% to C. fusca cultures resulted in a 31.6% increase in the lipid productivity. These results showed that C. fusca can be used for CO2 bioconversion and for producing biomass with potential applications for biofuels and bioproducts.
Combining pretreatment plasma Epstein‐Barr virus DNA level and cervical node necrosis improves prognostic stratification in patients with nasopharyngeal carcinoma: A cohort study
Authors: Du Yu‐Yun , Luo Dong‐Hua , Sun Xue‐Song , Tang Lin‐Quan , Mai Hai‐Qiang , Chen Qiu‐Yan , Zhong Jing‐Hua , Mai Dong‐Mei , Zhang Wan‐Ru , Chen Wen‐Hui , Mo Hao‐Yuan 
Published: September 2019

AbstractThis study aimed to evaluate the prognostic value of combining pretreatment Epstein‐Barr virus (EBV) DNA level and cervical node necrosis (CNN) for patients with nasopharyngeal carcinoma (NPC) receiving intensity‐modulated radiotherapy (IMRT). A total of 607 incident nonmetastatic NPC patients treated with IMRT ± chemotherapy were reviewed. Patients were divided into four groups based on EBV DNA level and CNN status. The primary endpoint was progression‐free survival (PFS). Kaplan‐Meier curves with log‐rank test were applied to compare survival outcomes and the Cox proportional model was used to identify independent prognostic factors. Pretreatment EBV DNA level and CNN status were independent prognostic factors. Patients in the low‐level EBV DNA group or non‐CNN group had significantly better 5‐year PFS. Multivariate analyses demonstrated that CNN was an independent prognostic factor for overall survival (OS) (HR = 1.927, 95% CI: 1.129‐3.290, P = .016), PFS (HR = 1.492, 95% CI: 1.005‐2.214, P = .047), distant metastasis‐free survival (DMFS) (HR = 1.661, 95% CI: 1.044‐2.644, P = .032), but not locoregional relapse‐free survival. EBV DNA levels correlated significantly with CNN with a correlation coefficient of .324 (P < .001). Compared with low‐level EBV DNA and non‐CNN grouping, high‐level EBV DNA and CNN grouping had poor PFS. The combined classification was an independent prognostic factor for OS (P < .001), PFS (P = .001), and DMFS (P = .018). Pretreatment plasma EBV DNA level and CNN status both closely correlated with prognosis of NPC patients in the IMRT era. Combined EBV DNA level and CNN status improves risk stratification and prognostic value.
Synthesis, Characterization and Evaluation of Cytotoxic Activities of Novel Aziridinyl Phosphonic Acid Derivatives
Authors: Khan Rehan , Ulusan Sinem , Banerjee Sreeparna , Dogan Özdemir 
Published: September 2019

AbstractNew aziridine 2‐phosphonic acids were prepared by monohydrolysis of the aziridine 2‐phosphonates that were obtained by the modified Gabriel−Cromwell reaction of vinyl phosphonate or α‐tosylvinyl phosphonate with a primary amine or a chiral amine. The cellular cytotoxicity of these compounds was tested against the HCT‐116 colorectal cancer cell lines and the CCD‐18Co normal colon fibroblast lines using the MTT assay. Three of the synthesized phosphonic acid derivatives 2e (ethyl hydrogen {(2S)‐1‐[(1S)‐1‐(naphthalen‐2‐yl)ethyl]aziridin‐2‐yl}phosphonate), 2h (ethyl hydrogen (1‐benzylaziridin‐2‐yl)phosphonate), and 2i (ethyl hydrogen (1‐cyclohexylaziridin‐2‐yl)phosphonate) showed higher cytotoxicity than the reference cancer treatment agent etoposide. Cell death was through a robust induction of apoptosis even more effectively than etoposide, a well‐known apoptosis inducing agent.
Silencing long noncoding RNA OGFRP1 inhibits the proliferation and migration of cervical carcinoma cells
Authors: Zou Kun , Yu Haifeng , Chen Xuehua , Ma Qian , Hou Lifang 
Published: September 2019

Cervical cancer is still a serious threat to women's health and life safety worldwide, and new treatment strategies are urgently needed. Accumulating evidences also imply that long non‐coding RNAs (lncRNAs) are involved in a wide range of cellular processes, such as cell proliferation, apoptosis, and cell cycle. We found that the expression of lncOGFRP1 in cervical cancer tissues was significantly higher than that in normal cervical tissues (P < .05). Further, CCK8 detection found when lncOGFRP1 was silenced, the proliferation of cells was inhibited. After depleting lncOGFRP1, the proportion of apoptosis cells in C33A (3.71 ± 0.38% VS 11.98 ± 1.26%, P < .05) and SiHa (0.69 ± 0.06% VS 11.06 ± 1.03%, P < .05) cells increased significantly, and cell cycle was arrested in S phase. On the other hand, migration detection found the migration of cells also was hindered when lncOGFRP1 level was reduced. And the depletion of lncOGFRP1 inhibited the expression of β‐catenin, Vimentin, N‐cadherin, and SNAIL and promoted the expression of E‐cadherin. In summary, we first discovered the high expression of lncOGFRP1 in cervical cancer and revealed that silencing lncOGFRP1 inhibits the proliferation and migration of cervical carcinoma cells.Significance of the studyWe first discovered the high expression of lncOGFRP1 in cervical cancer and revealed that silencing lncOGFRP1 inhibits the proliferation and migration of cervical carcinoma cells. These results help to better understand the pathogenesis and development of cervical cancer and provide insight to develop better diagnosis and treatment strategies.
Insight into the Mechanism of Phenylacetate Decarboxylase (PhdB), a Toluene‐Producing Glycyl Radical Enzyme
Authors: Rodrigues Andria V. , Tantillo Dean J. , Mukhopadhyay Aindrila , Keasling Jay D. , Beller Harry R. 
Published: September 2019

AbstractWe recently reported the discovery of phenylacetate decarboxylase (PhdB), representing one of only ten glycyl‐radical‐enzyme reaction types known, and a promising biotechnological tool for first‐time biochemical synthesis of toluene from renewable resources. Here, we used experimental and computational data to evaluate the plausibility of three candidate PhdB mechanisms, involving either attack at the phenylacetate methylene carbon or carboxyl group [via H‐atom abstraction from COOH or single‐electron oxidation of COO− (Kolbe‐type decarboxylation)]. In vitro experimental data included assays with F‐labeled phenylacetate, kinetic studies, and tests with site‐directed PhdB mutants; computational data involved estimation of reaction energetics using density functional theory (DFT). The DFT results indicated that all three mechanisms are thermodynamically challenging (beyond the range of many known enzymes in terms of endergonicity or activation energy barrier), reflecting the formidable demands on PhdB for catalysis of this reaction. Evidence that PhdB was able to bind α,α‐difluorophenylacetate but was unable to catalyze its decarboxylation supported the enzyme's abstraction of a methylene H atom. Diminished activity of H327A and Y691F mutants was consistent with proposed proton donor roles for His327 and Tyr691. Collectively, these and other data most strongly support PhdB attack at the methylene carbon.
Procedural, clinical, and health status outcomes in chronic total coronary occlusion revascularization: Results from the PERSPECTIVE study
Authors: Kandzari David E. , Lembo Nicholas J. , Carlson Harold D. , Kalynych Anna , Spertus John A. , Gibson C. Michael , Chi Gerald , Morgan Jayne , Rinehart Sarah , Yehya Amin , Qian Zhen , Ajose Bola , Karmpaliotis Dimitri 
Published: September 2019

BackgroundLimited research has detailed the outcomes of chronic total occlusion (CTO) percutaneous coronary intervention (PCI) with independent core laboratory and event adjudication. This study examined procedural, clinical, and patient‐reported health status outcomes among patients undergoing CTO PCI with specific focus on outcomes for those treated with zotarolimus‐eluting stents (ZES).MethodsAmong 500 consecutive patients undergoing attempted CTO PCI, procedural and in‐hospital clinical outcomes were examined in addition to the 1‐year composite endpoint of death, myocardial infarction, and target lesion revascularization (major adverse cardiac events, MACE). In a pre‐specified cohort of 250 patients, health status measures were ascertained at baseline and 1 year. A powered secondary endpoint was 1‐year MACE among patients treated with ZES compared with a performance goal.ResultsDemographic, lesion, and procedural characteristics for the overall population included prior bypass surgery, 29.8%; diabetes, 35.2%; occlusion length >20 mm, 71.3%; J‐CTO score, 2.5 ± 1.1; and primary retrograde strategy, 30.8%. Overall guidewire crossing was 90.9%; clinical success following guidewire crossing, 94.3%; and 1‐year MACE rate, 12.1%. One‐year health status significantly improved from baseline with successful CTO‐PCI (angina frequency, 72.7 ± 26.5 at baseline to 96.0 ± 10.8, p < .0001). Compared with a performance goal derived from prior CTO DES trials (1‐year hierarchal MACE, 25.2%), treatment with ZES was associated with significantly lower MACE (18.2%, one‐sided upper CI, 23.6%, p = .017).ConclusionsFavorable procedural success, health status improvements and late‐term clinical outcomes inform the relative risks and benefits of CTO PCI when performed in a clinically indicated, complex patient population representative of those treated in clinical practice.
Predictors of survival in patients with acute coronary syndrome undergoing percutaneous coronary intervention of unprotected left main coronary artery stenosis
Authors: Staudacher Dawid L. , Schmitt Charlotte , Zirlik Andreas , Zehender Manfred , Stachon Peter , Bothe Wolfgang , Zotzmann Viviane , Bode Christoph , von zur Muehlen Constantin 
Published: September 2019

AbstractObjectiveAim of this study was to investigate predictors of survival in unstable patients with high SYNTAX‐1‐score.BackgroundIn significant unprotected left main coronary artery (ULMCA) stenosis, treatment options include percutaneous coronary intervention (PCI) and coronary artery bypass graft (CABG). While CABG is recommended for stable patients with ULMCA stenosis and a SYNTAX‐1‐score > 32, PCI may be preferable in unstable or high operative risk patients.MethodsRetrospective single‐center all‐comers registry study.ResultsA total of 142 patients underwent ULMCA‐PCI (~72.9 years, 23.2% females, 54.2% survival in 2‐year follow‐up), 84 of whom had a SYNTAX‐1 > 32 (37.4 ± 12.8). Patients in the high‐SYNTAX‐1‐group (score > 32) were more often in an acute condition compared to low‐SYNTAX‐2‐group (score ≤ 32) including acute myocardial infarction (76.2% vs. 57.4%, p = .024), cardiogenic shock (48.2% vs. 14.8%, p = .001), or need for mechanical support (36.1% vs. 11.1%, p = .001). Survival was predicted by the acute condition including cardiogenic shock (OR 0.06 and 0.05) and myocardial infarction (OR 0.03 and 0.34) in both groups. Performance of the SYNTAX‐1‐score was limited in our patient collective in both groups (c‐index 0.65 vs. 0.63) while SYNTAX‐2‐PCI‐score performed better (c‐index 0.67 vs. 0.67). EuroScore II had the best discriminative ability (c‐index 0.87 vs. 0.78).ConclusionsThe majority of patients undergoing ULMCA‐PCI presented in acute conditions with high SYNTAX‐1‐score, and is therefore underrepresented in clinical trials. Prognosis was best predicted by the acute condition and the EuroScore II. These data suggest that therapy in unstable patients should be guided by clinical condition over the anatomical SYNTAX‐1‐score.
Engineering resilience with precast monolithic refractory articles
Authors: Goski Dana , Lambert Matthew 
Published: September 2019

AbstractPrecast refractory ceramic shapes have had well‐known benefits over other refractory lining methods: consistent and controlled manufacturing processes with faster turnaround than traditional cast‐in‐place monolithic refractories, reduced labor cost over classic brick installations, and, if well designed, simple repair assemblies. By analyzing the common failure modes of precast monolithic shapes, a distinct method to enhance these advantages was theorized. Through modeling and in‐field testing, the use of multicomponent and heterogeneous composite refractory systems incorporating high‐temperature reinforcement structures increased reliability and provided more predictable modes of failure.
One‐Step Photochemical Green Synthesis of Water‐Dispersible Ag, Au, and Au@Ag Core–Shell Nanoparticles
Authors: Perez‐Lloret Marta , Fraix Aurore , Petralia Salvatore , Conoci Sabrina , Tafani Virginie , Cutrone Giovanna , Vargas‐Berenguel Antonio , Gref Ruxandra , Sortino Salvatore 
Published: September 2019

AbstractA simple and green synthetic protocol for the rapid and effective preparation of Ag, Au and Au@Ag core–shell nanoparticles (NPs) is reported based on the light irradiation of a biocompatible, water‐soluble dextran functionalized with benzophenone (BP) in the presence of AgNO3, HAuCl4, or both. Photoactivation of the BP moiety produces the highly reducing ketyl radicals through fast (<50 ns) intramolecular H‐abstraction from the dextran scaffold, which, in turn, ensures excellent dispersibility of the obtained metal NPs in water. The antibacterial activity of the AgNPs and the photothermal action of the Au@Ag core–shell are also shown.
Fluorescence Properties and Exciplex Formation of Emissive Naphthyridine Derivatives: Application as Sensors for Amines
Authors: Hirota Junko , Usui Kazuteru , Fuchi Yasufumi , Sakuma Masaomi , Matsumoto Shota , Hagihara Ryusuke , Karasawa Satoru 
Published: September 2019

AbstractWater‐soluble donor–acceptor‐type fluorophore 15Nap‐Cl having two trifluoromethyl groups and a Cl group on a 1,5‐aminonaphthyridine framework was prepared. Fluorophore 15Nap‐Cl showed strong solvatochromic fluorescence, and, as the solvent polarity increased, a bathochromic shift was observed accompanied by an increase in the fluorescence quantum yield. In addition, in the presence of amines such as ethylamine, diethylamine, and aniline, further considerable bathochromic shifts in the fluorescence were observed. Density functional calculations identified the source of the fluorescence behavior as exciplex formation between 15‐Nap‐Cl and the corresponding amine. The fluorescence behavior was exploited to fabricate a sensor that can identify various primary, secondary, and tertiary amines.