bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inferring the genetic architecture of expression variation from replicated high throughput allele-specific expression experiments
Authors: Zhang Xinwen , Emerson J.J. 
Published: July 2019

AbstractGene expression variation between alleles in a diploid cell is mediated by variation in cis regulatory sequences, which usually refers to the differences in DNA sequence between two alleles near the gene of interest. Expression differences caused by cis variation has been estimated by the ratio of the expression level of the two alleles under a binomial model. However, the binomial model underestimates the variance among replicated experiments resulting in the exaggerated statistical significance of estimated cis effects and thus many false discoveries of cis-affected genes. Here we describe a beta-binomial model that estimates the cis-effect for each gene while permitting overdispersion of variance among replicates. We demonstrated with simulated null data (data without true cis-effect) that the new model fits the true distribution better, resulting in approximately 5% false positive rate under 5% significance level in all null datasets, considerably better than the 6%-40% false positive rate of the binomial model. Additional replicates increase the performance of the beta-binomial model but not of the binomial model. We also collected new allele-specific expression data from an experiment comprised of 20 replicates of a yeast hybrid (YPS128/RM11-1a). We eliminated the mapping bias problem with de novo assemblies of the two parental genomes. By applying the beta-binomial model to this dataset, we found that cis effects are ubiquitous, affecting around 70% of genes. However, most of these changes are small in magnitude. The high number of replicates enabled us a better approximation of cis landscape within species and also provides a resource for future exploration for better models.
Metabolic wastes are extracellularly disposed by excretosomes, nanotubes and exophers in mouse HT22 cells through an autophagic vesicle clustering mechanism
Authors: Fu Hualin , Li Jilong , Du Peng , Jin Weilin , Cui Daxiang 
Published: July 2019

AbstractNeurodegenerative diseases are characterized by metabolic waste accumulations in neuronal cells. However, little is known about the mechanism of metabolic waste disposal. We showed that there is a constitutive waste excretion by nanotubes and structures called excretosomes in mouse neuronal HT22 cells under normal conditions and an exopher-type waste disposal under stressed conditions. Excretosomes and exophers are waste-enriched multivesicular bodies that are formed by autophagic vesicle clustering and fusions. Upon H2O2treatment, metabolic waste marker intensified 1.4 to 3.4 fold. However, in dying H2O2-treated cells, glycolysis and stress response markers, alpha-Tubulin, Amyloid beta and alpha-Synuclein intensities reduced 30% to 80%. Amyloid beta, TAU and alpha-Synuclein associated with the autophagic vesicle clustering and fusion process and were excreted in large exophers by live oxidative-stressed cells before cell death with important implications for amyloid plaque, ghost tangle and Lewy body formation. H2O2treatment also induced exophers containing nucleic acid materials and significantly increased the level of oxidative damage marker 8-OHdG. Oxidative stress-induced HT22 cell death could be due to the combinations of excretion failure, cytoskeleton disruption, defective glycolysis and DNA damage.
Novel surface plasmon resonance biosensor that uses full-length Det7 phage tail protein for rapid and selective detection of Salmonella enterica serovar Typhimurium
Authors: Hyeon Seok Hywan , Lim Woon Ki , Shin Hae Ja 
Published: July 2019

AbstractWe report a novel surface plasmon resonance (SPR) biosensor that uses the full-length Det7 phage tail protein (Det7T) to rapidly and selectively detect Salmonella enterica serovar Typhimurium. Det7T, which was obtained using recombinant protein expression and purification in Escherichia coli, demonstrated a size of ∼75 kDa upon SDS-PAGE and was homotrimeric in its native structure. Micro-agglutination and TEM data revealed that the protein specifically bound to the host, S. Typhimurium, but not to non-host E. coli K-12 cells. The observed protein agglutination occurred over a concentration range of 1.5∼25 μg.ml−1. The Det7T proteins were immobilized on gold-coated surfaces using amine-coupling to generate a novel Det7T-functionalized SPR biosensor, wherein the specific binding of these proteins with bacteria was detected by SPR. We observed rapid detection of (∼ 20 min) and typical binding kinetics with S. Typhimurium in the range of 5 × 104-5 × 107 CFU.ml−1, but not with E. coli at any tested concentration, indicating that the sensor exhibited recognition specificity. Similar binding was observed with 10% apple juice spiked with S. Typhimurium, suggesting that this strategy could be expanded for the rapid and selective monitoring of target microorganisms in the environment.
Corporate governance in the shipping industry: board interlocks and agency conflicts
Authors: Andrikopoulos Andreas , Georgakopoulos Andreas , Merika Anna , Merikas Andreas 
Published: July 2019

PurposeThis paper aims to explore the effect of interlocking directorates on agency conflicts and corporate performance in the shipping industry.Design/methodology/approachThe authors use social network analysis to discover central nodes in the network of personal and corporate connections in an international sample of 110 listed shipping companies.FindingsAssessing network structure, the authors find that the network of corporate leaders is denser than the network of shipping companies. The network of shipping companies is populated with many isolated nodes; the network of shipping executives and directors is populated with many cohesive groups in which the longest distance between two corporate leaders is two companies. The authors find that interlocking corporate leadership can help resolve agency conflicts in the shipping industry, bearing a negative effect on the magnitude of agency costs. The extent of leadership overlaps is associated with board size, financial leverage and profitability. The relationship between profits and interlocks is bidirectional, implying that interlocking directorates bear a positive effect on asset returns.Originality/valueThe authors map the relational structures in the social networks of companies and company leaders in the shipping industry and discover the cross-sectional determinants of interlocks in the shipping industry. The finding about the effect of interlocks on profitability and agency costs bears policy implications for the design of corporate governance in the shipping industry.
Rural community tourism and sustainable advantages in Nicaragua
Authors: Artal-Tur Andres , Briones-Peñalver Antonio Juan , Bernal-Conesa Juan Andrés , Martínez-Salgado Oscar 
Published: July 2019

PurposeRural community tourism (RCT) represents an experience of community-based tourism where local population retains control over the process and the bulk of benefits. The purpose of this paper is to focus on the study of successful RCT experiences in Nicaragua to enlarge the literature of tourism sustainability.Design/methodology/approachApplying the resource-based theory of the firm to tourism, the paper defines a theoretical framework where local resources and capabilities combined through organization and strategic actions result in competitive advantages at the community level reinforcing its sustainable dimension. The model is tested empirically through Structural Equation Modelling-Partial Least Squares (SEM-PLS) modelling for Nicaraguan RCT experiences.FindingsMain findings show a good performance of empirical results, with the community dimension representing the cornerstone of the RCT project. Results remark how the presence of community tangible and intangible resources and capabilities are combined and exploited in tourism initiatives through strategies that put the preservation of the community as the central objective. This process leads to the emergence of competitive advantages that promote the sustainability of the community lifestyle, ensuring a durable approach of the rural tourism initiatives. Other interesting findings show how this type of RCT projects also promote the integration of weak rural collectives, like women and young people, or the pivotal cooperation emerging between public and private actors.Originality/valueThe paper provides a novel framework to better understand some of the key pieces ensuring the sustainability of tourism initiatives. This theoretical setting has been applied to the case of rural areas at developing countries but could be enlarged to other contexts at developed countries having to deal with mass tourism and important related negative impacts of these activities. In sum, the main value of the paper is to provide a framework helping to identify the context that is needed to implement successful sustainable tourism experiences.
It’s not what you do; it’s the way that you do it
Authors: D’Annunzio-Green Norma , Ramdhony Allan 
Published: July 2019

Purpose
This paper aims to draw on the key tenets of self-determination theory (SDT) to explore the possibility of deploying talent management (TM) as an inherently motivational process within the hospitality industry and examine the role of managers in leveraging it.

Design/methodology/approach
The study is rooted in social constructionism and uses qualitative methods and techniques to provide rich insights into employee perceptions and experiences of TM and related managerial attitudes and behaviours.

Findings
Although the current TM process is skewed towards performance outcomes, compelling evidence indicates variation in attempts to address employees’ motivational needs mediated by highly influential managerial attitudes and behaviours and importantly, suggests ample scope for embedding TM as an inherently motivational process.

Research limitations/implications
The findings are based on a relatively small sample but can be extrapolated with moderation to the wider research context and other similar organisational settings.

Practical implications
The paper develops an operational framework which contains clear guidelines that can be effectively translated into practice keeping in view its potential benefits.

Social implications
In line with SDT, the study foregrounds the social and relational context conducive to TM as a motivational process.

Originality/value
The paper is the first of its kind to conceptualise TM as an inherently motivational process via the systematic application of SDT and offers early empirical insights into the phenomenon, which can serve as a solid platform for further research.
Digital communication in and beyond organizations: unintended consequences of new freedom
Authors: Entschew Elisa Maria 
Published: July 2019

Purpose
The purpose of this paper is to address the following question: In times of permanent connectivity, what forms of freedom need to be considered to prevent permanent availability as an unintended consequence? By using the Hegelian perspective on freedom, the paper categorizes three forms of freedom to transfer them to a common, contemporary understanding of freedom relating it to freedom through human-to-human digital communication. The aim is to show that freedom is not only about independence and realizing choices but also about embedding and committing oneself.

Design/methodology/approach
This mainly conceptual paper derives implications based on the Hegelian theory. This is supplemented by an interdisciplinary approach, whereby categories of other philosophers, ethicists, economists and sociologists are applied. The analysis of the contemporary perspective on freedom is enriched by referencing empirical studies.

Findings
Digital communication offers new freedom such as working with fewer restrictions from time and space, especially for knowledge workers. It is theoretically possible to work 24 h per day from anywhere (independence), as well as to decide on the final location and timing of one’s work (realizing choices). When solely focusing on these – seemingly advantageous – forms of freedom in times of permanent connectivity, unintended consequences such as the expectation of permanent availability develop. The key message of the paper is that considering one’s temporal and social dependencies (embeddedness) is an indispensable part of actual freedom to avoid unintended consequences.

Practical implications
Organizations need to invest in moral discernment to understand unintended consequences, as well as to cope with them.

Originality/value
Applying the Hegelian theory on freedom based on digital communication to better understand social dynamics of digital communication is a largely unexplored avenue in the existing scientific literature. The decision to undertake this venture resulted from the identified necessity of understanding freedom better. It is often not clear what is meant by freedom through digital communication. Although freedom is a complex construct, it is often reduced to independence/having a choice and realizing choices. When solely focusing on independence and realizing choices, unintended consequences such as permanent availability often go unnoticed. It is exactly because of these issues that this paper endeavors to examine the (deep) meaning of the powerful, yet complex, term of freedom.
The prospects of Islamic banking in Southeast of Nigeria
Authors: Ezeh Precious Chikezie , Nkamnebe Anayo D. 
Published: July 2019

PurposeWith the increasing presence of Islamic banking to Nigeria’s banking space and its relative success in the predominantly Muslim Northern region, the question of its prospect in the predominantly non-Muslim southeastern Nigeria becomes legitimate and urgent. Thus, the purpose of this study is to predict the prospect of Islamic banking in Southeast Nigeria.Design/methodology/approachThree research questions were posed to address this objective. First, is there significant relationship between knowledge of Islamic banking concept and its prospects in Southeast Nigeria? Second, is there significant relationship between the relative advantage (principles) of Islamic banking concept and its prospects in Southeast Nigeria? Third, is there significant relationship between customers’ religion and prospects of Islamic banking concept in Southeast Nigeria? The study analyses using Pearson correlation, factor analysis with regression,t-test and ANOVA.FindingsKnowledge of Islamic banking and prospects of Islamic banking show weak positive relationship and very low mean. In other words, Islamic banking is not well-known; therefore, the prospects of Islamic banking slightly depend on knowledge about Islamic banking. Furthermore, the relationship between Islamic principles and prospects of Islamic banking shows only 5 per cent impact, which means that factors other than Islamic principle will drive the prospects of Islamic banking in Southeast Nigeria. Two factors loading from factor analysis are “profit sharing” and “forbid of some business activities”. Finally, profit sharing influences the prospects of Islamic banking, while prospects of Islamic banking will be impeded by customers’ religion.Research limitations/implicationsThe study was conducted in Southeast Nigeria which contains a small size of sample; the research adopted convenient sampling technique and a limited number of measures in the model. Nevertheless, the study provides new information about an emerging Islamic market.Originality/valueMost previous studies concentrated on product attributes of innovation adoption or patronage. But this current study inculcated the consumers' religion, as it affects the prospects of Islamic banking in Southeast Nigeria. .
“Part of being human”: evaluating the 4Ps model to support inpatient staff teams in reflecting on interpersonal dynamics
Authors: Jones Lindsay , Annesley Phyllis 
Published: July 2019

PurposeAn innovative training approach was developed to enable staff working with complex cases, including personality disorder, to reflect on and work with the interpersonal dynamics of their interactions with service users. The aim of this approach is to support effective, compassionate and boundaried care. An overview of the model and development of the training is provided along with presentation and discussion of outcome data. Implications for future practice are also considered.Design/methodology/approachOne-day workshops were provided within inpatient forensic women’s services. Nine workshops were delivered with 96 multidisciplinary staff having attended in total. Evaluation tools were developed to ascertain participants’ feedback regarding the training including its relevance and potential for impact on practice. Feedback was analysed using a mixture of quantitative and qualitative methods.FindingsThe evaluation demonstrated that the training was well received by a motivated group of participants and was felt to be relevant to their clinical practice.Research limitations/implicationsThe evaluation is limited by the lack of a follow-up to assess the longer-term impacts of the training and whether the positive effects of the training were maintained.Practical implicationsThe findings demonstrate that the training can be delivered within a short time frame, which makes the training efficient and cost effective.Social implicationsThe training can develop practitioners’ skills in delivering compassionate and boundaried care in line with key NHS drivers for staff working with complex service users.Originality/valueThe 4Ps model enables staff with little or no psychotherapy training to deliver psychologically informed care which takes account of interpersonal dynamics and positively contributes to relational security, with an emphasis on reflecting on self and others.
Thermal behaviour of a novel non-composite cellular beam floor system in fire
Authors: Marx Hendrig , Walls Richard 
Published: July 2019

Purpose
The Southern African Institute of Steel Construction has developed a novel cellular beam structure (CBS) for multi-storey buildings that is entirely devoid of concrete. Channel sections between the cellular beams support a complex sandwich flooring system, which contains a fire-resistant ceiling board, metal sheeting, an interior fibre-cement board and an access-flooring system. As for all structures, the CBS requires a fire rating. This paper aims to investigate the thermal behaviour of the CBS using numerical modelling and experimental fire testing, as it has a unique setup.

Design/methodology/approach
Experimental fire tests on the flooring system were conducted to validate finite element models, which were developed in ABAQUS. These models were then extended to include floor beams and the structural steelwork.

Findings
Good correlations were found between the experimental and numerical results, with temperature variations typically in the range of 0-5%, although with localised differences of up to 20%. This allowed larger finite element models, representing the sandwich floor system of the CBS, to be developed and analysed. A 1-hour rating can be obtained by the system in terms of insulation and integrity requirements.

Practical implications
The CBS allows for more economical steel structures, due to the rapid construction of its modular panels. A suitable fire resistance will ensure the safety of the occupants and prevent major structural damage. Steelwork and flooring temperatures are determined which has allowed for global structural analyses to be carried out.

Originality/value
The originality of this study lies in thermal analysis and testing of a new cellular beam flooring system, through determining behaviour in fire, along with beam temperatures.
Microstructure and mechanical properties of reinforced polyamide 12 composites prepared by laser additive manufacturing
Authors: Liu Yanhui , Zhu Lingjie , Zhou Lei , Li Yongjiu 
Published: July 2019

PurposeThis paper aims to explore the influence of the reinforcement included either glass beads (GBs) or carbon fiber (CF) on the reinforced polyamide 12 (PA12) composite samples prepared by selective laser sintering (SLS).Design/methodology/approachIn this paper, the microstructure and mechanical properties are investigated, and the results are compared with those obtained for non-reinforced pure PA12 samples prepared by SLS.FindingsThe tensile fracture surface of the non-reinforced pure PA12 sample presents strong micro-deformation within the crack origination zone between the melted PA12 matrix and the un-melted PA12 particle cores. As a result, the pure PA12 sample exhibits the greatest maximum elongation. The maximum tensile strength is obtained for the CF reinforced sample because of the strengthening effect of CF and the relatively good bonding between CFs and the PA12 matrix. The minimum tensile strength is obtained for the GB reinforced PA12 sample because of the relatively weak bonding between GBs and the PA12 matrix.Originality/valueThese results demonstrate that the characteristics of the interfaces between the reinforcement and the PA12 matrix have an important influence on the fracture mechanisms and mechanical properties of PA12 composites fabricated by SLS.
Improving responses to juvenile burglary offenders
Authors: Roth Jeffrey J. , Pierce Mari B. 
Published: July 2019

Purpose
The purpose of this paper is to make evidence-based recommendations for improving the responses of criminal justice agencies to juvenile burglary offenders.

Design/methodology/approach
The paper first analyzes what is known about factors relevant to young offenders’ initiation into burglary and subsequent persistence in that offense. It then evaluates research regarding juvenile justice interventions that can mitigate those factors in order to prevent youth from becoming involved in burglary or to encourage desistance in juvenile burglars.

Findings
Effective early intervention with juvenile burglars is vital, as burglars often begin committing this crime in their early teens and quickly develop expertise in the offense. Evidence supports the importance of positive mentoring, substance abuse programs, some forms of restorative justice and multi-modal interventions with education and employment components, while waiving these youth to adult court appears to offer little benefit over less punitive approaches.

Originality/value
This work delivers an original contribution by providing an analysis of existing burglary and juvenile justice research that may be useful to policymakers, law enforcement and other justice practitioners.