bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification of oil palm (Elaeis guineensis Jacq.) chitinases and their response to Ganoderma boninense Pat. infection
Authors: Aditama R , Tryono R , Tanjung Z A , Utomo C , Liwang T 
Published: July 2019

Abstract
The basal stem rot disease caused by Ganoderma boninense Pat. is a serious problem in oil palm plantation. Naturally, plant cells produce chitinases to protect themselves from chitin-containing parasites, particularly fungi. This study employed the advance of sequencing technology and availability of oil palm genomic and transcriptomic data to identify all chitinases in oil palm genome. Homology study using the combination of BLAST and HMM methods successfully identified 35 chitinases in oil palm which comprised of 22 GH18 and 13 GH19 family members. A multiple sequence alignment method classified those chitinases into class I, II, III, IV, V, VII and an unknown class. Chitinase domains analysis against PFAM databases showed more than half of chitinases possess partial domains of GH 18 or GH 19 in their sequences. Transcriptome analysis revealed that most of GH 18 family members were expressed specifically in roots under G. boninense infection treatment. Differently, most of GH 19 members were expressed constitutively in any tissues and under biotic stress conditions. This suggests that some GH 18 members might play an important role during host defence mechanism
Legal Change in the Face of Risk-Averse Subjects: A Generalization of the Theory
Authors: Franzoni Luigi Alberto 
Published: July 2019

Abstract
This study investigates the optimal nature of lawmaking under uncertainty. I focus on a case in which a harmful activity will be subjected to some regulatory measures (a standard, exposure to liability, or a corrective tax). The benefits and costs of precaution are ex ante uncertain, and this places a risk burden on both injurers and victims. The optimal policy should, at the same time, strike a balance between benefits and costs of the measures, and attenuate the ex ante risk. Whether measures should be made stronger or softer depends on the size and the sign of the shocks affecting the parties (positive or negative) and their disposition towards risk. With corrective taxes, it also depends on the elasticity of precautions with respect to the tax rate.
Functional analysis of the heterotrimeric NF-Y transcription factor complex in cassava disease resistance
Authors: He Xinyi , Liu Guoyin , Li Bing , Xie Yanwei , Wei Yunxie , Shang Sang , Tian Libo , Shi Haitao 
Published: July 2019

Abstract

Background and Aims
The nuclear factor Y (NF-Y) transcription factor complex is important in plant growth, development and stress response. Information regarding this transcription factor complex is limited in cassava (Manihot esculenta). In this study, 15 MeNF-YAs, 21 MeNF-YBs and 15 MeNF-YCs were comprehensively characterized during plant defence.

Methods
Gene expression in MeNF-Ys was examined during interaction with the bacterial pathogen Xanthomonas axonopodis pv. manihotis (Xam). The yeast two-hybrid system was employed to investigate protein–protein interactions in the heterotrimeric NF-Y transcription factor complex. The in vivo roles of MeNF-Ys were revealed by virus-induced gene silencing (VIGS) in cassava.

Key Results
The regulation of MeNF-Ys in response to Xam indicated their possible roles in response to cassava bacterial blight. Protein–protein interaction assays identified the heterotrimeric NF-Y transcription factor complex (MeNF-YA1/3, MeNF-YB11/16 and MeNF-YC11/12). Moreover, the members of the heterotrimeric NF-Y transcription factor complex were located in the cell nucleus and conferred transcriptional activation activity to the CCAAT motif. Notably, the heterotrimeric NF-Y transcription factor complex positively regulated plant disease resistance to Xam, confirmed by a disease phenotype in overexpressing plants in Nicotiana benthamiana and VIGS in cassava. Consistently, the heterotrimeric NF-Y transcription factor complex positively regulated the expression of pathogenesis-related genes (MePRs).

Conclusions
The NF-Y transcription factor complex (MeNF-YA1/3, MeNF-YB11/16 and MeNF-YC11/12) characterized here was shown to play a role in transcriptional activation of MePR promoters, contributing to the plant defence response in cassava.
Flowers up! The effect of floral height along the shoot axis on the fitness of bat-pollinated species
Authors: Diniz Ugo M , Domingos-Melo Arthur , Machado Isabel Cristina 
Published: July 2019

AbstractBackground and AimsBat-pollination is an important system in terms of occurrence and distribution, although it remains little studied. Thus, the role of particular flower traits in this interaction remains uncertain. Flower height along the shoot axis, associated with flower exposure, has often been deemed a key trait in this system, but its effect on fitness has not previously been assessed. We aimed to test its role and propose that taller flowers attain higher fitness due to a higher degree of accessibility and conspicuity to foraging bats.MethodsWe assessed the effect of floral height on bat visiting rates to individual flowers of Crescentia cujete (Bignoniaceae), a cauliflorous model bat-pollinated species with a marked gradient in flower height along the shoot axis. Additionally, we tested the effect of this variable on seed/ovule ratio measurements from seven other species from different families along a herb–tree gradient. Hypotheses were tested through mixed-effect linear models.Key ResultsBat visiting rates varied positively as a function of flower height in C. cujete, but significance was found only for the subset of flowers located on the trunk, closer to the ground. Similarly, seed/ovule ratios were positively correlated with flower height only for the three species with the shortest statures along the height gradient and shortest average floral heights. These results suggest that proximity to the ground, associated with herbaceous or bushy surrounding vegetation, may be an obstacle to the foraging of nectar-feeding bats, which in turn should explain the morphological convergence of inflorescence length and exposure strategies of short-statured bat-pollinated plants.ConclusionsFlower height has a species-specific effect on plant fitness. This study provides a novel numerical perspective to the role of an unexplored trait in bat-pollination, and has elucidated some aspects of the adaptive importance of flower height based on limitations imposed by ecologically complex pollinators.
Ontogenetic and ecotypic variation in the coloration and morphology of rainbow trout (Oncorhynchus mykiss) in a stream–lake system
Authors: Arostegui M C , Quinn T P 
Published: July 2019

AbstractAlternative ecotypes of diverse animal taxa exhibit distinct, habitat-specific phenotypes. Rainbow trout (Oncorhynchus mykiss), a salmonid fish, exhibits stream-resident (fluvial), lake-migrant (adfluvial) and ocean-migrant (anadromous) ecotypes throughout its range. We investigated the coloration, and morphology associated with swimming performance of wild, native non-anadromous rainbow trout in connected stream and lake habitats of a south-west Alaskan watershed to assess if they exhibited phenotypic diversity consistent with the presence of alternative fluvial and adfluvial ecotypes. Colour differences among rainbow trout of different size classes and habitats (stream or lake) indicated ecotype-specific pathways, diverging at the same point in ontogeny and resulting in different terminal coloration patterns. Specifically, lake-caught fish exhibited distinct silvering of the body, whereas stream-caught fish displayed banded coloration when small and bronze colour when larger. The morphology of lake-caught rainbow trout also differed from that of stream-caught fish in features associated with swimming performance, and they exhibited both shared and unique morphological patterns compared to sympatric Salvelinus species in those habitats [Dolly Varden (S. malma) in streams, and Arctic char (S. alpinus) in the lake]. Greater morphological variation within stream- than lake-caught rainbow trout, and their limited overlap in morphology, suggested population-specific partial migration. This study highlights the intraspecific diversity of migratory behaviour and how conservation of particular phenotypes depends on managing both for genotypes and for habitats.
Bioethics and Human Flourishing: Christian Wisdom in a Secular Age
Authors: Iltis Ana 
Published: July 2019

AbstractThe gulf between Christian and secular bioethics has far-reaching implications for public policy, healthcare organizations, clinicians, and patients and their families. There also are significant differences among various Christian approaches to bioethics. Differences and similarities between Christian and secular bioethics as well as among Christian approaches to bioethics are evident across three domains explored in this issue of Christian Bioethics. The first concerns different approaches to or methods for resolving ethical questions. The second concerns the ways in which understandings of health and disease and human anthropology shape our judgments about what we may do in the pursuit of health or in response to disease. The third concerns how our perceptions of and regard for others affect judgments of moral worth and can influence healthcare decision-making.
Purpose and Providence: An Outline for Christian Practical Wisdom in Health Care
Authors: Kaldjian Lauris Christopher 
Published: July 2019

Abstract
Decision-making in health care is often challenging and therefore requires practical wisdom. The domains of such wisdom involve goals, perception, ethics, deliberation, and motivation. For Christian patients, there is a need for practical wisdom founded on Christian commitments that shape and guide these domains according to a Christian understanding of life, health, technology, illness, suffering, and death. In this essay, I outline a Christian approach to practical wisdom in health care by infusing Christian beliefs and values into a general framework for practical wisdom in medicine I have described previously. I organize this approach by referring to Christian purpose, vision, ethics, piety, and practice. The result is a framework that intends to integrate faith and reason so that what we believe as Christians forms how we think about health and guides what we choose or decline when considering the possibilities that come before us in health care.
Association between marital status and insomnia-related symptoms: findings from a population-based survey in Japan
Authors: Kawata Yumi , Maeda Mitsuya , Sato Tomoyo , Maruyama Koutatsu , Wada Hiroo , Ikeda Ai , Tanigawa Takeshi 
Published: July 2019

Abstract

Background
Marital status is one of the socio-economic factors associated with health. Several studies have indicated a significant association between marital status and insomnia. The increases in the percentages of unmarried people in Japan are expected to produce a significant impact on insomnia. The purpose of this study was to examine the association between marital status and insomnia.

Methods
The participants were 35 288 people aged 30–59 years selected from the 2010 comprehensive survey of living conditions. We categorized marital status into five groups: single, married couples living with other family members, married couples living without other family members, widowed and divorced. Insomnia-related symptoms (IRS) were based on the participants who chose the answer, ‘I couldn’t sleep’. Sex-specific multivariable odds ratios (ORs) and 95% confidence intervals (CI) of IRS according to marital status were calculated using the logistic regression model, which was adjusted for potential confounding factors.

Results
The proportions of people with IRS were 2.5% in men and 2.8% in women. The multivariable ORs (95% CI) were 1.15 (0.89–1.49) for single, 1.69 (1.11–2.58) for divorced and 1.01 (0.73–1.39) for married couples living without other family members in men, and 1.56 (1.20–2.03) for single, 2.43 (1.83–3.22) for divorced and 1.31 (1.01–1.71) for married couples living without other family members in women.

Conclusions
We found divorced men and single, divorced and married women living without other family members had higher IRS than those who were married couples living with other family members in Japanese. This association was more evident in unemployed men.
Effects of Arbuscular Mycorrhiza Fungi on the Growth Characteristics, Root Morphology, and Ion Distribution of Pyrus betulaefolia Bunge under Saline-Alkaline Stress
Authors: Yang Xiuyan , Li Huanyong , Jiang Lei , Tang Xiaoqian , Liu Xiaowei , Zhang Huaxin 
Published: July 2019

Abstract
In this study, Pyrus betulaefolia Bunge seedlings were used to investigate the effects of an arbuscular mycorrhiza fungus (AMF; Funneliformis mosseae) on plant-growth characteristics, root morphology, salt tolerance, and ion distribution under saline-alkaline stress. An indoor pot experiment was conducted in which seedlings were inoculated with Funneliformis mosseae under treatments of four different concentrations of Na2CO3 solution. The results show that AMF could establish symbiotic relations with the P. betulaefolia root system under Na2CO3 stress. Under the same Na2CO3 stress, AMF significantly increased the height growth and biomass accumulation of P. betulaefolia seedlings. AMF also promoted the root growth of P. betulaefolia seedlings. AMF inoculation significantly affected the absorption and distribution of K+ and Na+ in P. betulaefolia. The K/Na ratios in the roots and leaves of seedlings inoculated with AMF were higher than those of seedlings without inoculation. The results of this study show that inoculation with AMF had a positive effect on enhancing the saline-alkaline tolerance of P. betulaefolia, and root treatment of P. betulaefolia seedlings with Funneliformis mosseae has the potential for application in the development of saline-alkaline soil vegetation.
Primary angiosarcoma of the breast presenting with a benign vascular skin-like lesion and expanding hematoma: a case report of an extremely rare tumor
Authors: Burusapat Chairat , wongprakob Nutthadon , Sapruangthong Rapeepat , Pruksapong Chatchai , Kaewkangsadan Viriya , Wongchansom Kittisak , Satayasoontorn Kantang 
Published: July 2019

AbstractPrimary breast angiosarcoma is an extremely rare tumor type (0.05% of primary breast cancers) for which diagnosis can be difficult. They arise within the breast parenchyma and typically present as a breast mass. Here, we present the case of a 30-year-old female with spontaneous hypervascular skin on her right breast with progressive enlargement presenting as an expanding hematoma. A chest computed tomography revealed a hypervascular mass in the right breast abutting the pectoralis muscle and cystic changes within the mass. A core needle biopsy revealed an angiosarcoma. In this case study, we report a patient who, with no history of any known risk factors, presented with a benign vascular skin lesion as the first sign of angiosarcoma followed by an expanding hematoma, which is an extremely rare manifestation of the disease. Microscopic examination demonstrated a low-grade angiosarcoma on the skin, while a high-grade angiosarcoma was found in the breast parenchyma.
Serum magnesium, hepatocyte nuclear factor 1β genotype and post-transplant diabetes mellitus: a prospective study
Authors: van der Burgh Anna C , Moes Arthur , Kieboom Brenda C T , van Gelder Teun , Zietse Robert , van Schaik Ron H N , Hesselink Dennis A , Hoorn Ewout J 
Published: July 2019

AbstractBackgroundRetrospective studies suggest that tacrolimus-induced hypomagnesaemia is a risk factor for post-transplant diabetes mellitus (PTDM), but prospective studies are lacking.MethodsThis was a prospective study with measurements of serum magnesium and tacrolimus at pre-specified time points in the first year after living donor kidney transplantation (KT). The role of single nucleotide polymorphisms (SNPs) in hepatocyte nuclear factor 1β (HNF1β) was also explored because HNF1β regulates insulin secretion and renal magnesium handling. Repeated measurement and regression analyses were used to analyse associations with PTDM.ResultsIn our cohort, 29 out of 167 kidney transplant recipients developed PTDM after 1 year (17%). Higher tacrolimus concentrations were significantly associated with lower serum magnesium and increased risk of hypomagnesaemia. Patients who developed PTDM had a significantly lower serum magnesium trajectory than patients who did not develop PTDM. In multivariate analysis, lower serum magnesium, age and body mass index were independent risk factors for PTDM. In recipients, the HNF1β SNP rs752010 G > A significantly increased the risk of PTDM [odds ratio (OR) = 2.56, 95% confidence interval (CI) 1.05–6.23] but not of hypomagnesaemia. This association lost significance after correction for age and sex (OR = 2.24, 95% CI 0.90–5.57). No association between HNF1β SNPs and PTDM was found in corresponding donors.ConclusionsA lower serum magnesium in the first year after KT is an independent risk factor for PTDM. The HNF1β SNP rs752010 G > A may add to this risk through an effect on insulin secretion rather than hypomagnesaemia, but its role requires further confirmation.
An unusual case of deep venous thrombosis in a young patient: congenital absence of the infrarenal portion of the inferior vena cava
Authors: Osborne Thomas , Sheehan Frances 
Published: July 2019

Abstract
Deep venous thrombosis (DVT) is a commonly encountered diagnosis in clinical practice with a variety of well-established risk factors. Congenital absence of the inferior vena cava (IVC) is an extremely rare but established risk factor for DVT. Patients who develop DVTs are at high risk of long-term complications, including DVT recurrence and post-thrombotic syndrome. Here we report a rare case of a 27-year-old female who presented with an extensive DVT of the right lower extremity secondary to complete absence of the infrarenal portion of the IVC, confirmed on computed tomography. There is little consensus regarding the appropriate management of this patient population, and a brief review of the current evidence follows.
Breast abscess in a competitive high school swimmer: a case of toxic shock syndrome
Authors: Taylor Gregory M , Vieder Sanford J , Cooley Christopher N , Fan Wiley C , Alper Michael E 
Published: July 2019

Abstract
Toxic shock syndrome (TSS) is a severe, acute, toxin-mediated disease process characterized by fever, diffuse erythroderma, hypotension, multisystem organ dysfunction and desquamation of skin. TSS represents the most severe form of disease caused by exotoxin-producing strains of Streptococcus pyogenes and Staphylococcus aureus. Menstrual and non-menstrual TSS become significant causes of morbidity and mortality. As a result of public awareness and various campaigns, the majority of TSS cases tend to be non-menstrual related. The clinical course is fulminant and can result in abrupt decompensation and death. Management within the emergency department (ED) includes removal of the potential foreign body, fluid resuscitation, appropriate antibiotics, potential vasopressor support and possible surgical intervention. We present the unique case of a 16-year-old female competitive swimmer who presented to the ED twice, demonstrating the fulminant course of TSS. She initially presented with non-specific symptoms with an unremarkable evaluation. She returned within hours of discharge with an abrupt onset of diffuse macular erythroderma, placed on norepinephrine and was diagnosed with TSS secondary to a breast abscess.
Altered structural brain network resulting from white matter injury in obstructive sleep apnea
Authors: Lee Min-Hee , Yun Chang-Ho , Min Areum , Hwang Yoon Ho , Lee Seung Ku , Kim Dong Youn , Thomas Robert J , Han Bong Soo , Shin Chol 
Published: July 2019

AbstractStudy ObjectivesTo assess, using fractional anisotropy (FA) analysis, alterations of brain network connectivity in adults with obstructive sleep apnea (OSA). Abnormal networks could mediate clinical functional deficits and reflect brain tissue injury.MethodsStructural brain networks were constructed using diffusion tensor imaging (DTI) from 165 healthy (age 57.99 ± 6.02 years, male 27.9%) and 135 OSA participants (age 59.01 ± 5.91 years, male 28.9%) and global network properties (strength, global efficiency, and local efficiency) and regional efficiency were compared between groups. We examined MRI biomarkers of brain tissue injury using FA analysis and its effect on the network properties.ResultsDifferences between groups of interest were noted in global network properties (p-value < 0.05, corrected), and regional efficiency (p-value < 0.05, corrected) in the left middle cingulate and paracingulate gyri, right posterior cingulate gyrus, and amygdala. In FA analysis, OSA participants showed lower FA values in white matter (WM) of the right transverse temporal, anterior cingulate and paracingulate gyri, and left postcentral, middle frontal and medial frontal gyri, and the putamen. After culling fiber tracts through WM which showed significant differences in FA, we observed no group difference in network properties.ConclusionsChanges in WM integrity and structural connectivity are present in OSA participants. We found that the integrity of WM affected brain network properties. Brain network analysis may improve understanding of neurocognitive deficits in OSA, enable longitudinal tracking, and provides explanations for specific symptoms and recovery kinetics.
Preadmission Statin Use and 90-day Mortality in the Critically Ill
Authors: Kyu Oh Tak , Song In-Ae , Lee Jae Ho , Lim Cheong , Jeon Young-Tae , Bae Hee-Joon , Jo You Hwan , Jee Hee-Jung 
Published: July 2019

Abstract

Editor’s Perspective

What We Already Know about This Topic

What This Article Tells Us That Is New

Background
This study aimed to examine the association between preadmission statin use and 90-day mortality in critically ill patients and to investigate whether this association differed according to statin type and dose. We hypothesized that preadmission statin use was associated with lower 90-day mortality.

Methods
This retrospective cohort study analyzed the medical records of all adult patients admitted to the intensive care unit in a single tertiary academic hospital between January 2012 and December 2017. Data including preadmission statin use, statin subtype, and daily dosage were collected, and the associations between these variables and 90-day mortality after intensive care unit admission were examined. The primary endpoint was 90-day mortality.

Results
A total of 24,928 patients (7,396 statin users and 17,532 non–statin users) were included. After propensity score matching, 5,354 statin users and 7,758 non–statin users were finally included. The 90-day mortality rate was significantly higher in non–statin users (918 of 7,758; 11.8%) than in statin users (455 of 5,354; 8.5%; P < 0.001). In Cox regression analysis, the 90-day mortality rate was lower among statin users than among non–statin users (hazard ratio: 0.70, 95% CI: 0.63 to 0.79; P < 0.001). Rosuvastatin use was associated with 42% lower 90-day mortality (hazard ratio: 0.58, 95% CI: 0.47 to 0.72; P < 0.001). There were no specific significant differences in the association between daily statin dose and 90-day mortality. In competing risk analysis, the risk of noncardiovascular 90-day mortality in statin users was 32% lower than that in non–statin users (hazard ratio: 0.68, 95% CI: 0.60 to 0.78; P < 0.001). Meanwhile, cardiovascular 90-day mortality was not significantly associated with statin use.

Conclusions
Preadmission statin use was associated with a lower 90-day mortality. This association was more evident in the rosuvastatin group and with noncardiovascular 90-day mortality; no differences were seen according to daily dosage intensity.