bluuebunny/crossref_metadata_2025_split huggingface dataset:
The RZZ complex integrates spindle checkpoint maintenance with dynamic expansion of unattached kinetochores
Authors: Rodriguez-Rodriguez Jose-Antonio , McKinley Kara L. , Sikirzhytski Vitali , Corona Jennifer , Maciejowski John , Khodjakov Alexey , Cheeseman Iain M. , Jallepalli Prasad V. 
Published: April 2018

SummaryThe Mad1-Mad2 heterodimer is the catalytic hub of the spindle assembly checkpoint (SAC), which controls mitosis through assembly of a multi-subunit anaphase inhibitor, the mitotic checkpoint complex (MCC) [1, 2]. Mad1-Mad2 first catalyzes MCC assembly at interphase nuclear pores [3], then migrates to kinetochores at nuclear envelope breakdown (NEBD) and resumes MCC assembly until bipolar spindle attachment is complete [1, 2]. There is significant debate about the factor(s) involved in targeting Mad1-Mad2 to kinetochores in higher eukaryotes [4-9]. Through gene editing and live-cell imaging, we found that the human Rod-Zw10-Zwilch (RZZ) complex is dispensable for cell viability and initial recruitment of Mad1-Mad2 to kinetochores at NEBD, but then becomes necessary to tether Mad1-Mad2 at kinetochores and sustain SAC arrest in cells challenged with spindle poisons. We also show that RZZ forms the mesh-like fibrous corona, a structural expansion of the outer kinetochore important for timely chromosome congression [10-13] once Mps1 phosphorylates the N-terminus of Rod. Artificially tethering Mad1-Mad2 to kinetochores enabled long-term mitotic arrest in the absence of RZZ. Conversely, blocking early RZZ-independent recruitment of Mad1-Mad2 eliminated the transient SAC response in RZZ-null cells. We conclude that RZZ drives structural changes in the outer kinetochore that facilitate chromosome bi-orientation and chronic SAC transduction, a key determinant of cytotoxicity during anti-mitotic drug therapy [14-16].
Homology Modeling and Docking Study of Shewanella Like Protein Phosphatase Involved in Development of Ookinetes in Plasmodium
Authors: Singh Sandhini , Yadav Ruchi 
Published: April 2018

ABSTRACTParasites of the genus Plasmodium cause a great deal of morbidity and mortality in worldwide, largely in regions with limited access and indication to the tools necessary to control mosquito populations and to treat human infections of Malaria. Five species of this class of eukaryotic pathogens cause different human disease, with Plasmodium falciparum alone is infecting approximately 500 million people per year and resulting in approximately 1 million deaths. The two genes encoding the Shewanella-like phosphatases of P.falciparum, SHLP-1 and SHLP-2, are conserved among members of Plasmodium family. SHLP is frequently found in asexual blood stages and expressed at all stages of the life cycle of parasite. SHLP deletion results in a reduction in microneme formation, ookinetes (zygote) development and complete ablation of oocyst formation, thereby blocking transmission of parasite.. Structure Modeling of SHLP protein can be helpful in understanding the active site and binding site information and hence can be used for drug designing for therapeutics against malaria. Effective role of Resveratrol is studied against SHLP protein using Docking method to identify protein-ligand interaction scheme and bond formation. Study suggests that resveratrol have strong interaction with SHLP protein and can be used as ligand for drug designing.
Nitro-Oleic Acid Induced Reactive Oxygen Species Formation and Plant Defense Signaling in Tomato Cell Suspensions
Authors: Arruebarrena Di Palma Andrés , Di Fino Luciano M. , Salvatore Sonia R. , Martín D’Ambrosio Juan , Grozeff Gustavo Esteban Gergoff , García-Mata Carlos , Schopfer Francisco J. , Laxalt Ana M. 
Published: April 2018

ABSTRACTNitrated fatty acids (NO2-FAs) are formed by the addition reaction of nitric oxide- and nitrite-derived nitrogen dioxide with unsaturated fatty acids. Nitrated fatty acids act as signaling molecules in mammals through the formation of covalent adducts with cellular thiols. The study of NO2-FAs in plant systems constitutes an interesting and emerging area. The presence of NO2-FA has been reported in olives, peas, rice and in Arabidopsis. To gain a better understanding of the role of NO2-FA on plant physiology, we analyzed the effects of exogenous application of nitro-oleic acid (NO2-OA) to tomato cell cultures. We found that NO2-OA induced reactive oxygen species (ROS) production in a dose-dependent manner via activation of NADPH oxidases, which requires calcium entry from the extracellular compartment and protein kinase activation, a mechanism that resembles the plant defense responses. NO2-OA-induced ROS production, expression of plant defense genes and led to cell death. The mechanism of action of NO2-OA involves a reduction in the glutathione cellular pool and covalently addition reactions with protein thiols and reduced glutathione. Altogether, these results indicate that NO2-OA triggers responses associated with plant defense, revealing its possible role as a signal molecule in biotic stress.Abbreviations•NO2nitrogen dioxide•NOnitric oxideFAfatty acidGSHreduced glutathioneH2O2hydrogen peroxydeNO2-FAnitro fatty acidsNO2-Lnnitro-linolenic acidNO2-OAnitro-oleic acidOAoleic acidROSreactive oxygen species
Nearshore corals on the Mesoamerican Barrier Reef System on pace to cease growing as soon as year 2110
Authors: Baumann Justin H. , Ries Justin B. , Rippe John P. , Courtney Travis A. , Aichelman Hannah E. , Westfield Isaac , Castillo Karl D. 
Published: April 2018

AbstractAnthropogenic global change and local anthropogenic stressors are decreasing coral growth and survival globally, thus altering the structure and function of coral reef ecosystems. We show that skeletal extension rates of nearshore colonies of Siderastrea siderea and Pseudodiploria strigosa across the Belize Mesoamerican Barrier Reef System (MBRS) have declined at average rates of 0.01 and 0.08 mm/yr, respectively, over approximately the past century, while offshore conspecifics exhibited no significant trend in extension with time. This caused extension rates of nearshore colonies to converge with their historically slower-growing offshore conspecifics. Bleaching events negatively impacted extension rates in S. siderea but not in P. strigosa. The more negative trend in linear extension for nearshore versus offshore colonies may arise from ocean warming combined with stronger land-based anthropogenic stressors within nearshore environments. Extrapolating these trends in linear extension into the future suggests that nearshore P. strigosa and S. siderea will cease growing by years 2110 and 2370, respectively.
Whence Lotka-Volterra? Conservation Laws and Integrable Systems in Ecology
Authors: O’Dwyer James P. 
Published: April 2018

AbstractCompetition in ecology is often modeled in terms of direct, negative effects of one individual on another. An example is logistic growth, modeling the effects of intraspecific competition, while the Lotka-Volterra equations for competition extend this to systems of multiple species, with varying strengths of intra- and inter-specific competition. These equations are a classic and well-used staple of quantitative ecology, providing a framework to understand species interactions, species coexistence, and community assembly. They can be derived from an assumption of random mixing of organisms, and an outcome of each interaction that removes one or more individuals. However, this framing is some-what unsatisfactory, and ecologists may prefer to think of phenomenological equations for competition as deriving from competition for a set of resources required for growth, which in turn may undergo their own complex dynamics. While it is intuitive that these frameworks are connected, and the connection is well-understood near to equilibria, here we ask the question: when can consumer dynamics alone become an exact description of a full system of consumers and resources? We identify that consumer-resource systems with this property must have some kind of redundancy in the original description, or equivalently there is one or more conservation laws for quantities that do not change with time. Such systems are known in mathematics as integrable systems. We suggest that integrability in consumer-resource dynamics can only arise in cases where each species in an assemblage requires a distinct and unique combination of resources, and even in these cases it is not clear that the resulting dynamics will lead to Lotka-Volterra competition.I acknowledge the Simons Foundation Grant #376199 and the McDonnell Foundation Grant #220020439.
Peptides derived from plant virus VPg protein inhibit eIF4E oncogene
Authors: Wojtal Izabela , Podsiadla-Bialoskorska Malgorzata , Grzela Renata , Bujak Malgorzata , Szolajska Ewa , Chroboczek Jadwiga 
Published: April 2018

AbstractViruses of the Potyviridae family have VPg protein covalently attached to the 5’ end of their linear RNA genome. The protein interacts with the host translation initiation factor eIF4E that occurs in plant cells in two isoforms, one being the preferable target of a given VPg, the remaining one still acting in host protein synthesis. In animal cells only one form of eIF4E is directly involved in protein synthesis. The human eIF4E is known to be an oncogene; elevated expression of eIF4E leads to oncogenic transformation, cancers in animal models and poor prognosis in human cancers, while reduction of the eIF4E level can reverse the transformed phenotype. We show that VPg protein delivery to cells containing only one eIF4E isoform involved in protein synthesis resulted in immobilization of eIF4E in the cytoplasm. The region of VPg involved in the interaction with eIF4E has been partially identified. Peptides derived from this region interacted better with eIF4E than complete VPg protein. Here we characterized one of VPg peptides, VPg5 and we show that VPg5 delivered to colon carcinoma HCT116 cells is able to inhibit cell growth, which is accompanied by reduction in eIF4E level.List of abbreviationsAcMNPVAutographa californica multicapsid nucleopolyhedrovirusCBBCoomassie Brilliant BlueClYVVClover yellow vein viruseIF4Eeukaryotic initiation translation factor 4E4EBP, eIF4E binding proteinFCSfetal calf serumLMVLettuce Mosaic VirusMOImultiplicity of infectionMWmolecular weightONovernightTMB3,3,5,5-tetramethylbenzidineT. niTrichoplusia ni cellsPBSTPBS buffer containing 0.05% Tween-20pipost infectionPIpropidium iodideTEVtobacco etch virusTVMVtobacco vein mottling virusPVYpotato virus YVPggenome-linked viral protein
Identifying policy entrepreneurs of public sector accounting agenda setting in Australia
Authors: Schührer Sabine 
Published: April 2018

Purpose
The purpose of this paper is to improve Kingdon’s (1984, 2011) concept of policy entrepreneurs (PE) with regard to the theoretical development of the definition and identification and level of agency by supplementing it with elements of Schmidt’s (2008, 2010, 2011, 2012) sentient agents. The improved concept of discursive policy entrepreneurs (DPEs) is then applied in an in-depth case study about the agenda setting process of micro and macro whole-of-government accounting in Australia in the late 1990s and early 2000s.

Design/methodology/approach
Based on the concept of DPEs, a series of operationalised characteristics and proxies are developed to identify them and describe their behaviour. These are then applied in the case study. The two main data sources are semi-structured in-depth interviews and archival documents.

Findings
The findings show that the focus on DPEs’ discursive and coordination activities is critical for identifying and investigating the key actors of the Generally Accepted Accounting Principles (GAAP)/Government Finance Statistics (GFS) harmonisation agenda setting process. The study also finds that the two relevant decision-making bodies, the Financial Reporting Council and the Australian Accounting Standards Board, lost control over their agendas due to the actions of DPEs.

Research limitations/implications
The improved concepts of DPEs will allow researchers to better identify the main agents of policy change and differentiate them from other supporters of policy ideas. Due to the qualitative nature of the study, the findings are not necessarily generalisable.

Practical implications
The findings from this study can help participants of agenda setting processes to gain a better understanding of the actions and behaviours of DPEs. This might allow standard setting bodies to mitigate against undue influences by DPEs.

Originality/value
This study is the first study that uses Schmidt’s concept of the sentient agent to address the limitations of Kingdon’s concept of PE and develops and applies characteristics to identify PEs and their actions. It is also the only study to date that investigates the GAAP/GFS harmonisation agenda setting process.
Governmental accounting practitioners: cardigan removed, research agenda revealed
Authors: Christensen Mark , Greiling Dorothea , Christiaens Johan 
Published: April 2018

Purpose
The purpose of this paper is to encourage research implicating public sector accounting practitioners. It overviews articles in the AAAJ Forum arising from the Comparative International Governmental Accounting Research (CIGAR) Network conference in 2015 in which practitioners’ doings were themes across numerous papers. The paper’s central objective is to scope out an agenda for future research in the area.

Design/methodology/approach
Using the CIGAR presentations and papers reviewed for this AAAJ Forum, a desk-based study informed by these sources and others has been conducted.

Findings
Aspects of public sector accounting practitioners’ doings hold promise in themselves whilst also being likely to complement and enrich other themes of public sector accounting research. Those aspects give rise to analytical frames, which may overlap and/or reinforce other aspects. Those analytical frames are: first, examining networking between practitioners; second, identifying implications of the professionalisation project for public sector accounting practitioners; third, analysing public sector accounting practitioners’ responses to the rise of external experts; and, fourth, exploring how public sector accounting practitioners interact with forces that shape the accounting craft. The four articles published here variously address several parts of these themes.

Originality/value
In scoping out a future research agenda, this paper justifies greater attention being paid to the four themes noted in its findings. In each of these research fields, an interdisciplinary approach is important.
Organisational talent management and perceived employer branding
Authors: Maurya Kamlesh Kumar , Agarwal Manisha 
Published: April 2018

PurposeThe purpose of this paper is to examine the potential impact of organisational talent management practices on perceived employer branding. Talented employees are the lifeline of any organisation who contribute effectively to the organisation’s success. Talented workforce and their organisational attraction to remain productive act as a primary competitive enabler for the organizational performance. Employer branding now becomes a key factor to achieve success in business which engenders cognitive and emotional association along with positive involvement at workplace among organizational members. In the context of economic competitiveness and globalisation, employers are experiencing challenge in attracting and retaining talented workers.Design/methodology/approachThis paper uses quantitative design; questionnaire survey method was used for assessing the organisational talent management and employer branding. Data have been collected through a sample of 232 executives’ class employees from various coal and iron mining industries located in Indian subcontinents. Finally, 197 responses were found to be acceptable for analysis. Hypotheses were tested using a comparison of means, correlations and multiple regression analysis.FindingsFindings of the paper show that organisational talent management is strongly and positively with the perceived employer branding. Regression analysis showed that among the eight dimensions of organisational talent management, the predictors that are most effective in predicting the employer branding are, namely, rewards and remunerates fairly, manages work–life balance and attracts and recruits talent.Research limitations/implicationsThis paper was limited by a small sample size and the use of a cross-sectional design is not done. On the ground of analysis, more specific approach is required to apply the findings to the general population.Originality/valueMuch of the work on employer branding is conceptual based on marketing principle and limited to deal with potential employees. This paper provides empirical evidence from the internal/current employees’ perspective, by investigating the relationship between organisational talent management practices and organisational attraction internally, that is, perceived employer branding. Present paper contributes significantly to the implication and understanding of social exchange theory, internal branding theory and social identity theory in organisational setting (Eisenberger et al., 1986; Lidenet al., 1997; Masterson et al., 2000; Settoon et al., 1996; Wayneet al., 1997).
Dynamics between social media engagement, firm-generated content, and live and time-shifted TV viewing
Authors: Viswanathan Vijay , Malthouse Edward C. , Maslowska Ewa , Hoornaert Steven , Van den Poel Dirk 
Published: April 2018

Purpose
The purpose of this paper is to study consumer engagement as a dynamic, iterative process in the context of TV shows. A theoretical framework involving the central constructs of brand actions, customer engagement behaviors (CEBs), and consumption is proposed. Brand actions of TV shows include advertising and firm-generated content (FGC) on social media. CEBs include volume, sentiment, and richness of user-generated content (UGC) on social media. Consumption comprises live and time-shifted TV viewing.

Design/methodology/approach
The authors study 31 new TV shows introduced in 2015. Consistent with the ecosystem framework, a simultaneous system of equations approach is adopted to analyze data from a US Cable TV provider, Kantar Media, and Twitter.

Findings
The findings show that advertising efforts initiated by the TV show have a positive effect on time-shifted viewing, but a negative effect on live viewing; tweets posted by the TV show (FGC) have a negative effect on time-shifted viewing, but no effect on live viewing; and negative sentiment from tweets posted by viewers (UGC) reduces time-shifted viewing, but increases live viewing.

Originality/value
Content creators and TV networks are faced with the daunting challenge of retaining their audiences in a media-fragmented world. Whereas most studies on engagement have focused on static firm-customer relationships, this study examines engagement from a dynamic, multi-agent perspective by studying interrelationships among brand actions, CEBs, and consumption over time. Accordingly, this study can help brands to quantify the effectiveness of their engagement efforts in terms of encouraging CEBs and eliciting specific TV consumption behaviors.
Service ecotones: the complex boundary zones of service (eco) systems
Authors: Simmonds Hamish , Gazley Aaron 
Published: April 2018

Purpose
The purpose of this paper is to introduce ecotones to the service literature as a conceptual extension of the service ecosystem (SE) framework.

Design/methodology/approach
To synthesise the contribution, an illustrative empirical case study with research of nine organisations and their service systems is developed.

Findings
Boundaries connect systems with their environment. Ecotone, a concept from natural ecosystems, provides a useful concept representing the boundary zones between adjacent systems, supporting emergent phenomena. The authors find that a service ecotone emerges through the interactions occurring at the intersections of relational, technological and institutional boundaries of two unique SEs. The case demonstrates ecotone edge effects – the unique landscape and diversity of actors and their roles – which play a role in the co-evolution of the separate SEs.

Practical implications
The ecotone concept provides an understanding of SE boundaries, helping practitioners understand the complex environments they operate. Developing strategy in complex ecosystems requires a clear understanding of where the boundaries of dependence and interdependence lie. The ecotone concept helps practitioners to develop responsiveness and resilience to their environment and take advantage of resources that may be currently unrecognised.

Originality/value
The authors introduce the ecotone concept and integrate it with service theory. This paper develops service ecotones for understanding the relationship between different systems that influence their functioning and development. Thus, ecotones suggest new avenues for understanding the diversity and roles of actors, and how new structural properties, resources and practices come to be through the tensions and interactions created in these complex boundaries of SEs.
Incorporating sustainability in small health-care facilities: an integrated model
Authors: Chu Kevin Wing Ki , Cheung Lenis Lai Wan 
Published: April 2018

Purpose
It is challenging for small health-care facilities to implement changes when human and financial resources are limited for day-to-day operations. This paper aims to propose an integrated model for small- and medium-sized health-care facilities to integrate sustainability in their day-to-day operations, which have been derived from the leadership and change theories.

Design/methodology/approach
Drawing on previous research on leadership and change theories, the paper first critically reviewed the approaches to implementing changes and how applicable they are in the context of small- and medium-sized health-care facilities. Next, it proposes an integrated model with an execution plan.

Findings
The first part of the paper discusses how either the planned approach or emergent approach for change may fail in facilitating the implementation of sustainable initiatives, as incorporating sustainability into operations require both leadership of change and open learning systems. The second part outlines the four-phase combined approach, which includes phases of “exploration”, “planning”, “action” and “integration”, and discusses how change readiness is ensured through such approach.

Practical implications
The authors propose an integrated model as a framework for integrating sustainability into the operations of small health-care facilities. The clearest possible steps at various phases are proposed. Potential barriers and risks are highlighted and the coping strategies proposed to maximise the chance of successfully transforming organisations.

Originality/value
Applying the “how to” ideas based on the integrated model for change management will help leaders of health-care facilities gradually integrate sustainability into their day-to-day operations.