bluuebunny/crossref_metadata_2025_split huggingface dataset:
Capillary electrophoresis/visible‐LED induced fluorescence of tryptophan: What's new?
Authors: Perquis Lucie , Ta Hai Yen , Ong‐Meang Varravaddheay , Poinso Alix , Collin Fabrice , Poinsot Véréna , Couderc François 
Published: June 2019

AbstractTryptophane (Trp) labelled by 3‐(4‐carboxybenzoyl)‐2‐quinolinecarboxaldehyde (CBQCA) is very difficult to identify using CE and fluorescence detection (480 nm). Why in this article some mass spectrometry experiments show that Trp is really labelled by CBQCA as Leucine (Leu)? If the maximum of UV absorption (λmax) is the same between Leu‐CBQCA and Trp‐CBQCA, the molar extinction coefficient is around 2 fold higher for Trp‐CBQCA. The fluorescence of the Leu‐CBQCA derivative is 50 times more important than for Trp‐CBQCA. The addition of 7.5 mM of β‐cyclodextrin (β‐CD) was found to be a good mean to improve 2.1 fold the sensitivity of the Trp‐CBQCA fluorescence. Using a buffer containing SDS and β‐CD in CE, a LOD of 0.7 µM of L‐Trp can be reached and the ratio of the intensities between Leu, Isoleucine, Valine, Trp is 100, 21, 15, 1. Negative ESI/MS and MS/MS of the labeled amino acids show that a loss of the carboxylate function takes place. In the presence of two enantiomers of Trp‐CBQCA, we have shown that this decarboxylation is not due to the derivatization process in the solution but rather occurs in the source of the mass spectrometer.
Comparison of the peptidome released from keratins in Saiga antelope horn and goat horn under simulated gastrointestinal digestion
Authors: Liu Rui , Zhu Zhenhua , Qian Dawei , Duan Jin‐ao 
Published: June 2019

AbstractGoat horn (Caprae Hircus Cornu, GH) has been used as a substitute for Saiga antelope horn (Saigae Tataricae Cornu, SAH) in the clinic and the pharmaceutical industry. In the present study, peptides released from SAH and GH under simulated gastric and intestinal digestion were identified. The results showed that most of the peptides released from SAH and GH under simulated gastrointestinal digestion were hydrophilic, and over 75% of the peptides from keratins (KRTs) were hydrophilic. In total, over 58% of the identified peptides were released from KRTs, and were from the four main regions of KRTs. The peptide features and the peptide release profiles from KRTs in SAH and GH were similar, which may provide a method for the identification of sustainable alternatives to replace the threatened SAH, and provide further evidence of the feasibility of using GH as a replacement for SAH based on their peptidomic analysis.
Method of localized removal of cells using a bolt‐clamped Langevin transducer with an ultrasonic horn
Authors: Inui Takumi , Kurashina Yuta , Imashiro Chikahiro , Takemura Kenjiro 
Published: June 2019

AbstractCell isolation by eliminating undesirable cell aggregations or colonies with low activity is essential to improve cell culture efficiency. Moreover, when creating tissues from induced pluripotent stem cells, residual undifferentiated cells must be removed to prevent tumor formation in vivo. Here, we evaluated the use of ultrasonic irradiation, which can apply energy locally without contact, and proposed a method to eliminate cells in a small area of culture by ultrasonic irradiation from a Langevin transducer. We constructed a device that incorporated a bolt‐clamped 19.84 kHz Langevin transducer with an ultrasonic horn and determined the optimal conditions for stable elimination of cells in small areas of a 35‐mm culture dish. The optimal conditions were as follows: number of cycles = 400, clearance distance = 1 mm, volume of medium = 4 mL, and distance from the center of culture surface = 0 mm. The mean cell elimination area under these conditions was 0.097 mm2. We also evaluated the viability of neighboring cells after ultrasonic irradiation by fluorescent staining and found that most cells around the elimination area survived. These findings suggest that the proposed method has potential for localized elimination of cells without the need for contact with the cell surface.
Experimental investigation on influencing factors of CO2huff and puff under fractured low‐permeability conditions
Authors: Li Binfei , Bai Hao , Li Aishan , Zhang Liaoyuan , Zhang Qiliang 
Published: June 2019

AbstractThe fracture system is a vital component of fractured low‐permeability reservoirs. The presence of fractures can improve reservoir flow capacity and injected carbon dioxide (CO2) utilization, thus leading to higher oil recovery. In this study, the effects of the presence of fracture, fracture morphology, soaking time, andCO2injection volume onCO2huff and puff were investigated through 11 low‐permeability cores with different properties. The experimental results indicated that the presence of fractures enhanced cyclic oil recovery and increased the effective cycle numbers duringCO2huff and puff in low‐permeability cores. Moreover, compared with low‐permeability cores without fractures, ultimate oil recovery ofCO2huff and puff was risen up by ~11% in fractured cores. Longer soaking time was conducive to enhancing ultimate oil recovery ofCO2huff and puff in fractured low‐permeability cores, but the excessive soaking time had little effect on ultimate oil recovery. Meanwhile, excessiveCO2injection volume did not significantly improve the performance ofCO2huff and puff, but it did reduce theCO2utilization. Moreover, gravity caused the produced oil to deposit on the bottom surface of the blowout end of the fracture, which made oil recovery of the core with a horizontal fracture slightly higher (~7%) than that of the core with a vertical fracture. In addition, variation in the intersection angle of fractures had little effect on ultimate oil recovery ofCO2huff and puff in fractured low‐permeability cores. It, however, did change the conductivity of the entire core, thus affecting oil recovery during the first two cycles remarkably.
Growth-Correcting the Bioconcentration Factor and Biomagnification Factor in Bioaccumulation Assessments
Authors: Gobas Frank A.P.C. , Lee Yung-Shan 
Published: June 2019

Abstract
We illustrate that the Organisation for Economic Co-operation and Development guideline 305 (OECD-305) for growth-correcting bioconcentration factors (BCFs) and biomagnification factors (BMFs) violates the mass-balance assumption underlying the definition of BCFs and BMFs and provides unrealistic estimates of BCFs and BMFs of chemicals in nongrowing fish. We present and test alternative methods for growth-correcting BCFs and BMFs that maintain mass balance. We conclude that the OECD-305-recommended growth correction of BCFs and BMFs causes error, is unnecessary, and should be revisited. Environ Toxicol Chem 2019;38:2065–2072. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC.
Abstract
Effect of growth on bioconcentration factors and biomagnification factors.
Improving the Match Between Sustainability Questions and Evaluation Practice: Some Reflections and a Checklist
Authors: Chelimsky Eleanor 
Published: June 2019

AbstractThere is increasing evaluative interest in assessing not just the current effectiveness of programs but also their likely sustainability. Assessing the likely sustainability of a program, however, requires methodological judgments about the future and about the influences of many complex political, economic, and social factors, which themselves are likely to change. While strong confidence in these types of prospective judgments may continue to be elusive, careful consideration of some major factors—including the cyclical character of certain public interest values and the predictable influence of special interest groups—should be helpful in improving the current ability of evaluation practice to address sustainability issues. This chapter ends with a checklist of seventeen questions to help focus attention on relevant, accessible, but often ignored, program or policy areas affecting sustainability.
A smoke segmentation algorithm based on improved intelligent seeded region growing
Authors: Zhao Wangda , Chen Weixiang , Liu Yujie , Wang Xiangwei , Zhou Yang 
Published: June 2019

SummaryImage segmentation method based on region growing has the advantages of simple segmentation method and complete segmentation target. Because the color discrimination and gray gradient of smoke are not obvious, the traditional region growing segmentation method is difficult to separate it from the image, resulting in an unsatisfactory segmentation effect. To solve this problem, this paper partially improved the region growing method and proposed a new smoke segmentation algorithm based on the improved intelligent seeded region growing (IISRG) method. First, smoke images obtained from experimental videos were converted from the RGB color space to the HSV color space, and image binarization was achieved using background subtraction with an adaptive threshold in the V channel. Then, a pixel in the binary image was selected intelligently as the seed point, which was used for the regional growth. The final smoke segmentation images were obtained by the morphological processing of region growing images. Experimental smoke segmentation results show that the proposed algorithm has a higher overlap rate and a lower overflow rate, and performs a better smoke segmentation effect compared with the other two approaches. In addition, this algorithm can also effectively solve the problems of under‐segmentation and over‐segmentation of smoke images.
Ontogenetic oligodendrocyte maturation through gestational iron deprivation: The road not taken
Authors: Guitart María E. , Vence Marianela , Correale Jorge , Pasquini Juana M. , Rosato‐Siri María V. 
Published: June 2019

AbstractDevelopmental iron deficiency (dID) models facilitate the study of specific oligodendrocyte (OL) requirements for their progression to a mature state and subsequent contribution to myelination. In the current work, we used the dID model in transgenic mice expressing green fluorescence protein under the CNPase promoter allowing the identification of cells belonging to the oligodendroglial lineage, and the visualization of the entire myelin structure and single OL morphology. The present work evaluates dID effects on OL complexity in different brain areas. Control animals showed an increase in OL complexity both during development and along the anterior–posterior axis. In contrast, dID animals exhibited an initial increase in CNPase+ cells with prevalence of immature‐OL (i‐OL), an effect later compensated during development by selective death of those i‐OL. As a consequence, developmental behavior was impaired in terms of body balance, muscle response, and sensorimotor functions. To explore why i‐OL fail to mature in dID, expression levels of transcriptional factors involved in the maturation of the OL lineage were studied. In nuclear fractions, dID animals showed an increase in Hes5, which prevents the maturation of i‐OL, and a decrease in Sox10, a positive regulator of OL maturation. The cytoplasmic fractions showed a decrease in Olig1, which is critical for precursor cell differentiation into premyelinating OL. Overall, the expression levels of Hes5, Sox10, and Olig1 in dID conditions correlated with an unfavorable OL maturation profile. In sum, the current results provide further evidence of dID impact on myelination, keeping OL away from the maturational path.
Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues
Authors: Ayala Oscar D. , Doster Ryan S. , Manning Shannon D. , O'Brien Christine M. , Aronoff David M. , Gaddy Jennifer A. , Mahadevan‐Jansen Anita 
Published: June 2019

Abstract
Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a major cause of chorioamnionitis and neonatal sepsis. This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from Escherichia coli and Staphylococcus aureus during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis accurately identified GBS infected tissues with 100.0% sensitivity and 88.9% specificity. Together, these findings support further investigation into the use of RS as an emerging microbiologic diagnostic tool and intrapartum screening test for GBS carriage.
Recent progress in preparation and agricultural application of microcapsules
Authors: Li Ting , Teng Da , Mao Ruoyu , Hao Ya , Wang Xiumin , Wang Jianhua 
Published: June 2019

AbstractRecent advances in life science technology have prompted the need to develop microcapsule delivery systems that can encapsulate many different functional or active materials such as drugs, peptides, and live cells, etc. The encapsulation technology is now commonly used in medicine, agriculture, food, and other many fields. The application of biodegradable microcapsule systems can not only effectively prevent the degradation of core materials in the body or the biological environment, but also improve the bioavailability, control the release and prolong the halftime or storage of core active materials. Various wall materials, preparation methods, encapsulation processes, and release mechanisms are covered in this review, as well as several main factors including pH values, temperatures, particle sizes, and additives, which can strongly influence the encapsulation efficiency, the strength, and release of microcapsules. The improvement of coating materials, preparation techniques, and challenges are also highlighted, as well as application prospects.
Design and characterization of biodegradable multi layered electrospun nanofibers for corneal tissue engineering applications
Authors: Arabpour Zohreh , Baradaran‐Rafii Alireza , Bakhshaiesh Nasrin L. , Ai Jafar , Ebrahimi‐Barough Somayeh , Esmaeili Malekabadi Hossein , Nazeri Niloofar , Vaez Ahmad , Salehi Majid , Sefat Farshid , Ostad Seyed N. 
Published: June 2019

AbstractTissue engineering is one of the most promising areas for treatment of various ophthalmic diseases particularly for patients who suffer from limbal stem cell deficiency and this is due to the lack of existence of appropriate matrix for stem cell regeneration. The aim of this research project is to design and fabricate triple layered electrospun nanofibers as a suitable corneal tissue engineering scaffold and the objective is to investigate and perform various in vitro tests to find the most optimum and suitable scaffold for this purpose. Electrospun scaffolds were prepared in three layers. Poly(d, l‐lactide‐co‐glycolide; PLGA, 50:50) nanofibers were electrospun as outer and inner layers of the scaffold and aligned type I collagen nanofibers were electrospun in the middle layer. Furthermore, the scaffolds were cross‐linked by 1‐ethyl‐3‐(3 dimethylaminopropyl) carbodiimide hydrochloride and glutaraldehyde. Structural, physical, and mechanical properties of scaffolds were investigated by using N2 adsorption/desorption isotherms, Fourier transform infrared spectroscopy, contact angle measurement, tensile test, degradation, shrinkage analysis, and scanning electron microscopy (SEM). In addition, capability to support cell attachment and viability were characterized by SEM, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay, and 4′,6‐diamidino‐2‐phenylindole staining. According to the result of Brunauer–Emmett–Teller analysis, specific surface area of electrospun scaffold was about 23.7 m2 g‐1. Tensile tests on cross‐linked scaffolds represented more suitable hydrophilicity and tensile behavior. In addition, degradation rate analysis indicated that noncross‐linked scaffolds degraded faster than cross‐linked one and cross‐linking led to controlled shrinkage in the scaffold. The SEM analysis depicted nano‐sized fibers in good shape. Also, the in vitro study represented an improved cell attachment and proliferation in the presence of human endometrial stem cells for both cross‐linked and noncross‐linked samples. The current study suggests the possibility of producing an appropriate substrate for successful cornea tissue engineering with a novel design.
Comparing the effects of uncoated nanostructured surfaces on primary neurons and astrocytes
Authors: Liliom Hanna , Lajer Panna , Bérces Zsófia , Csernyus Bence , Szabó Ágnes , Pinke Domonkos , Lőw Péter , Fekete Zoltán , Pongrácz Anita , Schlett Katalin 
Published: June 2019

AbstractThe long‐term application of central nervous system implants is currently limited by the negative response of the brain tissue, affecting both the performance of the device and the survival of nearby cells. Topographical modification of implant surfaces mimicking the structure and dimensions of the extracellular matrix may provide a solution to this negative tissue response and has been shown to affect the attachment and behavior of both neurons and astrocytes. In our study, commonly used neural implant materials, silicon, and platinum were tested with or without nanoscale surface modifications. No biological coatings were used in order to only examine the effect of the nanostructuring. We seeded primary mouse astrocytes and hippocampal neurons onto four different surfaces: flat polysilicon, nanostructured polysilicon, and platinum‐coated versions of these surfaces. Fluorescent wide‐field, confocal, and scanning electron microscopy were used to characterize the attachment, spreading and proliferation of these cell types. In case of astrocytes, we found that both cell number and average cell spreading was significantly larger on platinum, compared to silicon surfaces, while silicon surfaces impeded glial proliferation. Nanostructuring did not have a significant effect on either parameter in astrocytes but influenced the orientation of actin filaments and glial fibrillary acidic protein fibers. Neuronal soma attachment was impaired on metal surfaces while nanostructuring seemed to influence neuronal growth cone morphology, regardless of surface material. Taken together, the type of metals tested had a profound influence on cellular responses, which was only slightly modified by nanopatterning.
TNFRSF11A-Associated Dysosteosclerosis: A Report of the Second Case and Characterization of the Phenotypic Spectrum
Authors: Xue Jing-yi , Wang Zheng , Shinagawa Satoshi , Ohashi Hirofumi , Otomo Nao , Elcioglu Nursel H , Nakashima Tomoki , Nishimura Gen , Ikegawa Shiro , Guo Long 
Published: June 2019

ABSTRACT
Dysosteosclerosis (DOS) is a distinct form of sclerosing bone disease characterized by irregular osteosclerosis and platyspondyly. DOS is genetically heterogeneous; however, only five cases with SLC29A3 mutations and a single case with a splice-site mutation of TNFRSF11A have been reported, and TNFRSF11A is also a causal gene for osteopetrosis, autosomal recessive 7 (OP-AR7). Thus, the causal genes of DOS and their genotype-phenotype associations remain unclear. In this study, we examined a Japanese patient with DOS and found a novel variant in TNFRSF11A. The homozygous variant was a G to T transversion at the first nucleotide of exon 9 (c.784G&gt;T). Although the variant was predicted to cause a stop codon mutation (p.E262*), in silico evaluation of the exonic splicing elements followed by RT-PCR for the patient-derived cells showed that it caused aberrant splicing due to the change in the exonic splicing element and produced two types of aberrant transcripts: One caused a premature stop codon (p.E262Vfs*17) leading to nonsense mutation-mediated mRNA decay; the other produced a protein with interstitial deletion (p.E262_Q279del). The effects of the mutation on five splicing isoforms of TNFRSF11A were different from those in OP-AR7, but comparable with those in the first DOS with the TNFRSF11A mutation. Thus, we identified the second case of DOS caused by the TNFRSF11A splice-site mutation and confirmed the novel disease entity. © 2019 American Society for Bone and Mineral Research.
MicroRNA‐374c‐5p inhibits the development of breast cancer through TATA‐box binding protein associated factor 7‐mediated transcriptional regulation of DEP domain containing 1
Authors: Hao Shuai , Tian Wuguo , Chen Yi , Wang Lingli , Jiang Yan , Gao Bo , Luo Donglin 
Published: June 2019

AbstractBreast cancer is the most pervasive cancer tormenting women, with increasing incidence and mortality rates year after year. MicroRNAs (miRNAs) with abnormal expression has various effects in biological processes and progression in diverse tumors. Nevertheless, it is vitally crucial for us to inspect more underlying molecular mechanisms for the therapy of patients with breast cancer. In the paper, we inquired the expression level and potential regulation mechanism of miR‐374c‐5p in breast cancer. Our research found out that miR‐374c‐5p was low‐level expressed in breast cancer. Upregulation of miR‐374c‐5p repressed cell proliferation, migration, and also epithelial‐mesenchymal transition (EMT), and induced cell apoptosis of breast cancer cells. Further, we concluded that miR‐374c‐5p interacted with TAF7 and downregulated its expression. Moreover, miR‐374c‐5p modulated DEP domain containing 1 (DEPDC1) through mediating TAF7. Finally, rescue assays represented that miR‐374c‐5p suppressed breast cancer development via TAF7‐mediated transcriptional regulation of DEPDC1. We uncovered that overexpressed miR‐374c‐5p inhibited the development of breast cancer via TAF7‐regulated transcriptional regulation of DEPDC1, which may be a novel and vital proportion of cancer diagnosis and treatment strategies.
lncRNA TUC338 is a potential diagnostic biomarker for bladder cancer
Authors: Li Gang , Zhang Yang , Mao Jing , Hu Peng , Chen Qiang , Ding Wei , Pu Rong 
Published: June 2019

AbstractAlthough long noncoding RNA TUC338 has been characterized as an oncogene, its role in bladder cancer is unknown. The purpose of the present study is to investigate the function of TUC338 in bladder cancer. We found that TUC338 was upregulated in early‐stage bladder cancer patients and showed early diagnostic values. After surgical resection, plasma levels of TUC338 were significantly downregulated. Moreover, microRNA 10b (miR‐10b) was also upregulated in bladder cancer patients. TUC338 and miR‐10b were positive and significantly correlated in bladder cancer patients, but not in healthy controls. Bladder cancer cells with TUC338 overexpression showed upregulated miR‐10b, while miR‐10b overexpression failed to significantly affect TUC338. TUC338 and miR‐10b overexpression significantly promoted bladder cancer cell invasion and migration. Therefore, TUC338 may promote bladder cancer at least partially by upregulating miR‐10b.
Implications of exosomes as diagnostic and therapeutic strategies in cancer
Authors: Aghebati‐Maleki Ali , Nami Sanam , Baghbanzadeh Amir , Karzar Bita H. , Noorolyai Saeed , Fotouhi Ali , Aghebati‐Maleki Leili 
Published: June 2019

AbstractExosomes offer a new perspective on the biology of cancer with both diagnostic and therapeutic concepts. Due to the cell‐to‐cell association, exosomes are involved in the progression, metastasis, and therapeutic efficacy of the tumor. They can be isolated from blood and other body fluids to determine the disease progression in the body, including cancer growth. In addition to being reservoirs of biochemical markers of cancer, exomes can be designed to restore tumor immunity. Tumor exosomes interact with different cells in the tumor microenvironment to confer beneficial modulations, responsible for stromal activity, angiogenesis, increased vascular permeability, and immune evasion. Exosomes also contribute to the metastasis with the aim of epithelial transmission to the mesenchyme and the formation of premetastatic niches. Moreover, exosomes protect cells against the cytotoxic effects of chemotherapeutic drugs and prevent the transmission of chemotherapy resistance to adjacent cells. Therefore, exosomes are essential for many fatal cancer agents, and understanding their origins and role in cancer is important. In this article, we attempted to clarify the potential of exosomes for the application in cancer diagnosis and therapy.