bluuebunny/crossref_metadata_2025_split huggingface dataset:
When Karl met Lollo: the origins and consequences of Karl Barth's relationship with Charlotte von Kirschbaum
Authors: Plant Stephen J. 
Published: April 2019

AbstractThis paper extends the scope of earlier studies of Karl Barth's relationship with Charlotte von Kirschbaum by exploring his marriage to Nelly Barth and the deterioration in their relationship in the 1920s. It traces the development and character of Barth's relationship with Kirschbaum, and the Notgemeinschaft (emergency community) they formed with Nelly. The paper makes use of Nelly Barth's unpublished letters to her family in which she accounts for her reluctance to divorce her husband in the early 1930s. The second half of the paper explores the impact of Barth's relationship with Kirschbaum on others and goes on to suggest ways in which this had consequences for his theology, particularly in his thinking in Church Dogmatics III/4 about the relation between man and woman.
Inseparable virtue and theimago Deiin Augustine: a speculative interpretation ofDe Trinitate6.4
Authors: Ployd Adam 
Published: April 2019

AbstractInDe Trinitate6.4, Augustine compares the inseparability of virtues within the human soul to the divine attributes within the simple divine substance of the Trinity. In this paper, I will suggest that this is more than a convenient analogy. Rather, I contend, the soul's virtues become inseparable as the soul itself conforms to the image of God through the primary virtue of love. My argument includes an analysis of the history of inseparable virtue in Graeco-Roman philosophy and a comparison of Augustine's use of the concept inTrin. 6.4 with his more extended treatment inEpistle167. In the face of a seeming conflict in these two texts, I argue for a ‘soft’ or ‘imperfect’ version of inseparability in Augustine's view of the virtues. Finally, I suggest that the cultivation of the virtues within the unity of love may be understood as the way we come to image the Trinity.
Church, theology and the holiness of God
Authors: Lösel Steffen 
Published: April 2019

AbstractThis paper addresses the identity crisis of Christian churches under the conditions of late modernity. With Jürgen Moltmann, I describe the dilemma of the contemporary church and of its theology as a crisis both of relevance and identity. I suggest that churches have responded to the loss of their stronghold in the Western world in three ways: liberal Protestants embrace modernity, evangelicals oppose it and a third group, whom I identify as church theologians, try to ignore it. I argue that none of the three approaches successfully solves the church's crisis in late modernity and especially in a consumer culture with its commodification of religion. I trace these struggles of the contemporary church to its loss of alterity, both of God and of the human other, and suggest that we can regain a sense of God's otherness by rediscovering God's holiness.
Is the child at risk? Cardiovascular remodelling in children born to diabetic mothers
Authors: Hoodbhoy Zahra , Mohammed Nuruddin , Aslam Nadeem , Fatima Urooj , Ashiqali Salima , Rizvi Arjumand , Pascua Christine , Chowdhury Devyani , Hasan Babar S. 
Published: April 2019

AbstractObjective:The objective of this study was to assess differences in myocardial systolic and diastolic function and vascular function in children 2−5 years of age born to diabetic as compared to non-diabetic mothers.Methods:This study was a retrospective cohort conducted in 2016 at The Aga Khan University Hospital, Karachi, Pakistan. It included children between 2 and 5 years of age born to mothers with and without exposure to diabetes in utero (n = 68 in each group) and who were appropriate for gestational age. Myocardial morphology and function using echocardiogram and carotid intima media thickness (cIMT) and pulse wave velocity was performed to evaluate cardiac function as well as macrovascular remodelling in these children. Multiple linear regression was used to compare the groups.Results:There was no significant difference in cardiac morphology, myocardial systolic and diastolic function, and macrovascular assessment between the exposed and unexposed groups of AGA children. Subgroup analysis demonstrated a significantly decreased mitral E/A ratio in children whose mothers were on medications as compared to those on dietary control (median [IQR] = 1.7 [1.6–1.9] and 1.56 [1.4–1.7], respectively, p = 0.02), and a higher cIMT in children whose mothers were on medication as compared to controls (0.48 [0.44–0.52] and 0.46 [0.44–0.50], respectively, p = 0.03).Conclusion:In utero exposure to uncontrolled maternal diabetes has an effect on the cardiovascular structure and function in children aged 2−5 years. However, future work requires long-term follow-up from fetal to adult life to assess these changes over the life course.
Effects of Tropical Deforestation on Surface Energy Balance Partitioning in Southeastern Amazonia Estimated From Maximum Convective Power
Authors: Conte Luigi , Renner Maik , Brando Paulo , Oliveira dos Santos Claudinei , Silvério Divino , Kolle Olaf , Trumbore Susan E. , Kleidon Axel 
Published: April 2019

AbstractTo understand changes in land surface energy balance partitioning due to tropical deforestation, we use a physically based analytical formulation of the surface energy balance. Turbulent heat fluxes are constrained by the thermodynamic maximum power limit and a formulation for diurnal heat redistribution within the land‐atmosphere system. The derived turbulent fluxes of sensible and latent heat compare very well to in situ observations for sites with intact rainforest and soybean land cover in southeastern Amazonia. The equilibrium partitioning into sensible and latent heat flux compares well with observations for both sites, except for the soybean site during the dry season where water limitation needs to be explicitly accounted for. Our results show that tropical deforestation primarily affects the absorption of solar radiation and the water limitation of evapotranspiration, but not the overall magnitude of turbulent heat fluxes that is set by the thermodynamic maximum power limit.
Groundwater Dependent Ecosystems in Arid Zones Can Use Ancient Subterranean Carbon as an Energy Source in the Local Food Web
Authors: Dean Joshua F. 
Published: April 2019

AbstractGroundwater dependent ecosystems (GDEs) are defined by their reliance on subterranean water resources, but GDE food webs may also depend on it as an energy source. Mazumder et al. (2019, https://doi.org/10.1029/2018JG004925), show a clear dependency of a local GDE food web on very old, potentially geological, carbon sourced from palaeo‐groundwater discharge in the arid Great Artesian Basin in Australia. These findings support the emerging paradigm that ancient groundwater carbon is an important component of inland water carbon cycling and contemporary ecosystem functioning. Future work is needed to determine what proportion of total GDE energy needs are derived from groundwater carbon across a range of geological and hydroclimatic settings.
Aryl hydrocarbon receptor agonist indigo protects against obesity-related insulin resistance through modulation of intestinal and metabolic tissue immunity
Authors: Lin Yi-Hsuan , Luck Helen , Khan Saad , Schneeberger Pierre H. H. , Tsai Sue , Clemente-Casares Xavier , Lei Helena , Leu Yann-Lii , Chan Yi Tao , Chen Hsing-Yu , Yang Sien-Hung , Coburn Bryan , Winer Shawn , Winer Daniel A. 
Published: April 2019

Abstract
Background/objectives
Low-grade chronic inflammation in visceral adipose tissue and the intestines are important drivers of obesity associated insulin resistance. Bioactive compounds derived from plants are an important source of potential novel therapies for the treatment of chronic diseases. In search for new immune based treatments of obesity associated insulin resistance, we screened for tissue relevant anti-inflammatory properties in 20 plant-based extracts.

Methods
We screened 20 plant-based extracts to assess for preferential production of IL-10 compared to TNFα, specifically targetting metabolic tissues, including the visceral adipose tissue. We assessed the therapeutic potential of the strongest anti-inflammatory compound, indigo, in the C57BL/6J diet-induced obesity mouse model with supplementation for up to 16 weeks by measuring changes in body weight, glucose and insulin tolerance, and gut barrier function. We also utilized flow cytometry, quantitative PCR, enzyme-linked immunosorbent assay (ELISA), and histology to measure changes to immune cells populations and cytokine profiles in the intestine, visceral adipose tissue (VAT), and liver. 16SrRNA sequencing was performed to examine gut microbial differences induced by indigo supplementation.

Results
We identifed indigo, an aryl hydrocarbon receptor (AhR) ligand agonist, as a potent inducer of IL-10 and IL-22, which protects against high-fat diet (HFD)-induced insulin resistance and fatty liver disease in the diet-induced obesity model. Therapeutic actions were mechanistically linked to decreased inflammatory immune cell tone in the intestine, VAT and liver. Specifically, indigo increased Lactobacillus bacteria and elicited IL-22 production in the gut, which improved intestinal barrier permeability and reduced endotoxemia. These changes were associated with increased IL-10 production by immune cells residing in liver and VAT.

Conclusions
Indigo is a naturally occurring AhR ligand with anti-inflammatory properties that effectively protects against HFD-induced glucose dysregulation. Compounds derived from indigo or those with similar properties could represent novel therapies for diseases associated with obesity-related metabolic tissue inflammation.
In vivo superresolution photoacoustic computed tomography by localization of single dyed droplets
Authors: Zhang Pengfei , Li Lei , Lin Li , Shi Junhui , Wang Lihong V. 
Published: April 2019

AbstractPhotoacoustic (PA) computed tomography (PACT) is a noninvasive hybrid imaging technique that combines optical excitation and acoustic detection to realize high contrast, high resolution, and deep penetration in biological tissues. However, the spatial resolution of PACT is limited by acoustic diffraction. Here, we report in vivo superresolution PACT, which breaks the acoustic diffraction limit by localizing the centers of single dyed droplets that are flowing in blood vessels. The droplets were prepared by dissolving hydrophobic absorbing dye in oil, followed by mixing with water. The dyed droplets generate much higher-amplitude PA signals than blood and can flow smoothly in vessels; thus, they are excellent tracers for localization-based superresolution imaging. The in vivo resolution enhancement was demonstrated by continuously imaging the cortical layer of a mouse brain during droplet injection. The droplets that were flowing in the vessels were localized, and their center positions were used to construct a superresolution image that exhibits sharper features and more finely resolved vascular details. An improvement in spatial resolution by a factor of 6 has been realized in vivo by the droplet localization technique.
Using Bacterial Transcriptomics to Investigate Targets of Host-Bacterial Interactions in Caenorhabditis elegans
Authors: Chan Jason P. , Wright Justin R. , Wong Hoi Tong , Ardasheva Anastasia , Brumbaugh Jamey , McLimans Christopher , Lamendella Regina 
Published: April 2019

AbstractThe interactions between a host and its resident microbes form complicated networks that can affect host physiology. Disentangling these host-microbe interactions can help us better understand mechanisms by which bacteria affect hosts, while also defining the integral commensal protection that host-associated microbiota offer to promote health. Here we utilize a tractable genetic model organism, Caenorhabditis elegans, to study the effects of host environments on bacterial gene expression and metabolic pathways. First, we compared the transcriptomic profiles of E. coli OP50 in vitro (on agar plates) versus in vivo (fed to C. elegans host). Our data revealed that 110 biosynthetic genes were enriched in host-associated E. coli. Several of these expressed genes code for the precursors and products needed for the synthesis of lipopolysaccharides (LPS), which are important for innate immune and stress responses, as well as pathogenicity. Secondly, we compared the transcriptomic profiles of E. coli fed to hosts with different genetic backgrounds, including the long-lived daf-2/insulin like growth factor (IGF) receptor and short lived daf-16/FOXO transcription factor mutants. We find that hosts genetics also alters bacterial metabolic pathways. Given that bacteria influence host health, this transcriptomics approach can elucidate genes mediating host aging.
G-quadruplex dynamics contribute to regulation of mitochondrial gene expression
Authors: Falabella M. , Kolesar J. E. , Wallace C. , de Jesus D. , Sun L. , Taguchi Y. V. , Wang C. , Wang T. , Xiang I. M. , Alder J. K. , Maheshan R. , Horne W. , Turek-Herman J. , Pagano P. J. , St. Croix C. M. , Sondheimer N. , Yatsunyk L. A. , Johnson F. B. , Kaufman B. A. 
Published: April 2019

AbstractSingle-stranded DNA or RNA sequences rich in guanine (G) can adopt non-canonical structures known as G-quadruplexes (G4). Mitochondrial DNA (mtDNA) sequences that are predicted to form G4 are enriched on the heavy-strand and have been associated with formation of deletion breakpoints. Increasing evidence supports the ability of mtDNA to form G4 in cancer cells; however, the functional roles of G4 structures in regulating mitochondrial nucleic acid homeostasis in non-cancerous cells remain unclear. Here, we demonstrate by live cell imaging that the G4-ligand RHPS4 localizes primarily to mitochondria at low doses. We find that low doses of RHPS4 do not induce a nuclear DNA damage response but do cause an acute inhibition of mitochondrial transcript elongation, leading to respiratory complex depletion. We also observe that RHPS4 interferes with mtDNA levels or synthesis both in cells and isolated mitochondria. Importantly, a mtDNA variant that increases G4 stability and anti-parallel G4-forming character shows a stronger respiratory defect in response to RHPS4, supporting the conclusion that mitochondrial sensitivity to RHPS4 is G4-mediated. Taken together, our results indicate a direct role for G4 perturbation in mitochondrial genome replication, transcription processivity, and respiratory function in normal cells.
Live-imaging of Bioengineered Cartilage Tissue using Multimodal Non-linear Molecular Imaging
Authors: Moura Catarina Costa , Bourdakos Konstantinos N. , Tare Rahul S. , Oreffo Richard O. C. , Mahajan Sumeet 
Published: April 2019

AbstractCoherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) are non-linear techniques that allow label-free, non-destructive and non-invasive imaging for cellular and tissue analysis. Although live-imaging studies have been performed previously, concerns that they do not cause any changes at the molecular level in sensitive biological samples have not been addressed. This is important especially for stem cell differentiation and tissue engineering, if CARS/SHG microscopy is to be used as a non-invasive, label-free tool for assessment of the developing neo-tissue. In this work, we monitored the differentiation of human fetal-femur derived skeletal cells into cartilage in three-dimensional cultures using CARS and SHG microscopy and demonstrate the live-imaging of the same developing neo-tissue over time. Our work conclusively establishes that non-linear label-free imaging does not alter the phenotype or the gene expression at the different stages of differentiation and has no adverse effect on human skeletal cell growth and behaviour. Additionally, we show that CARS microscopy allows imaging of different molecules of interest, including lipids, proteins and glycosaminoglycans, in the bioengineered neo-cartilage. These studies demonstrate the label-free and truly non-invasive nature of live CARS and SHG imaging and their value and translation potential in skeletal research, regeneration medicine and tissue engineering.
Automated drosophila heartbeat counting based on image segmentation technique on optical coherence tomography
Authors: Lee Chia-Yen , Wang Hao-Jen , Jhang Jheng-Da , Cho I-Chun 
Published: April 2019

AbstractDrosophila and human cardiac genes are very similar. Biological parametric studies on drosophila cardiac have improved our understanding of human cardiovascular disease. Drosophila cardiac consist of five circular chambers: a conical chamber (CC) and four ostia sections (O1–O4). Due to noise and grayscale discontinuity on optical coherence tomography (OCT) images, previous researches used manual counting or M-mode to analyze heartbeats, which are inefficient and time-consuming. An automated drosophila heartbeat counting algorithm based on the chamber segmentation is developed for OCT in this study. This algorithm has two parts: automated chamber segmentation and heartbeat counting. In addition, this study proposes a principal components analysis (PCA)-based supervised learning method for training the chamber contours to make chamber segmentation more accurate. The mean distances between the conical, second and third chambers attained by the proposed algorithm and the corresponding manually delineated boundaries defined by two experts were 1.26 ± 0.25, 1.47 ± 1.25 and 0.84 ± 0.60 (pixels), respectively. The area overlap similarities were 0.83 ± 0.09, 0.75 ± 0.11 and 0.74 ± 0.12 (pixels), respectively. The average calculated heart rates of two-week and six-week drosophila were about 4.77 beats/s and 4.73 beats/s, respectively, which was consistent with the results of manual counting.
HCV-coinfection is related to an increased HIV-1 reservoir size in cART-treated HIV patients: a cross-sectional study
Authors: López-Huertas Maria Rosa , Palladino Claudia , Garrido-Arquero Marta , Esteban-Cartelle Beatriz , Sánchez-Carrillo Marta , Martínez-Román Paula , Martín-Carbonero Luz , Ryan Pablo , Domínguez-Domínguez Lourdes , Santos Ignacio De Los , Moral Sara De La Fuente , Benito José Miguel , Rallón Norma , Alcamí José , Resino Salvador , Fernández-Rodríguez Amanda , Coiras Mayte , Briz Verónica ,   on behalf of the Multidisciplinary Group of viral coinfection HIV/Hepatitis (COVIHEP), Ángel-Moreno Alfonso , Bermejo-Plaza Laura , Bisbal Otilia , Brochado-Kith Oscar , Castro-Álvarez Juan Miguel , Cuevas Guillermo , Diez-Viñas Victorino , Gálvez-Charro Marta , García-Fraile Lucio , Gómez-Sanz Alicia , Lagarde María , Matarranz Mariano , Mate-Cano Irene , Mayoral-Muñoz Mario , Muñoz-Muñoz María , Pulido Federico , Rubio Rafael , Santacreu Mireia , Sanz-Sanz Jesús , Taveira Nuno , Troya Jesús , Cortegano Isabel , Gaspar María Luisa 
Published: April 2019

AbstractIn HIV-1/HCV-coinfected patients, chronic HCV infection leads to an increased T-lymphocyte immune activation compared to HIV-monoinfected patients, thereby likely contributing to increase HIV-1 reservoir that is the major barrier for its eradication. Our objective was to evaluate the influence of HCV coinfection in HIV-1 viral reservoir size in resting (r) CD4+ T-cells (CD25-CD69-HLADR-). Multicenter cross-sectional study of 97 cART-treated HIV-1 patients, including 36 patients with HIV and HCV-chronic co-infection without anti-HCV treatment, 32 HIV patients with HCV spontaneous clearance and 29 HIV-monoinfected patients. rCD4+ T-cells were isolated and total DNA was extracted. HIV viral reservoir was measured by Alu-LTR qPCR. Differences between groups were calculated with a generalized linear model. Overall, 63.9% were men, median age of 41 years and Caucasian. Median CD4+ and CD8+ T-lymphocytes were 725 and 858 cells/mm3, respectively. CD4+ T nadir cells was 305 cells/mm3. Proviral HIV-1 DNA size was significantly increased in chronic HIV/HCV-coinfected compared to HIV-monoinfected patients (206.21 ± 47.38vs. 87.34 ± 22.46, respectively;P = 0.009), as well as in spontaneously clarified HCV co-infected patients when compared to HIV-monoinfected individuals (136.20 ± 33.20;P = 0.009). HIV-1/HCV co-infected patients showed a larger HIV-1 reservoir size in comparison to HIV-monoinfected individuals. This increase could lead to a greater complexity in the elimination of HIV-1 reservoir in HIV-1/HCV-coinfected individuals, which should be considered in the current strategies for the elimination of HIV-1 reservoir.
Influence Maximization for Fixed Heterogeneous Thresholds
Authors: Karampourniotis P. D. , Szymanski B. K. , Korniss G. 
Published: April 2019

AbstractInfluence Maximization is a NP-hard problem of selecting the optimal set of influencers in a network. Here, we propose two new approaches to influence maximization based on two very different metrics. The first metric, termed Balanced Index (BI), is fast to compute and assigns top values to two kinds of nodes: those with high resistance to adoption, and those with large out-degree. This is done by linearly combining three properties of a node: its degree, susceptibility to new opinions, and the impact its activation will have on its neighborhood. Controlling the weights between those three terms has a huge impact on performance. The second metric, termed Group Performance Index (GPI), measures performance of each node as an initiator when it is a part of randomly selected initiator set. In each such selection, the score assigned to each teammate is inversely proportional to the number of initiators causing the desired spread. These two metrics are applicable to various cascade models; here we test them on the Linear Threshold Model with fixed and known thresholds. Furthermore, we study the impact of network degree assortativity and threshold distribution on the cascade size for metrics including ours. The results demonstrate our two metrics deliver strong performance for influence maximization.
Data mining to understand health status preceding traumatic brain injury
Authors: Mollayeva Tatyana , Sutton Mitchell , Chan Vincy , Colantonio Angela , Jana Sayantee , Escobar Michael 
Published: April 2019

AbstractThe use of precision medicine is poised to increase in complex injuries such as traumatic brain injury (TBI), whose multifaceted comorbidities and personal circumstances create significant challenges in the domains of surveillance, management, and environmental mapping. Population-wide health administrative data remains a rather unexplored, but accessible data source for identifying clinical associations and environmental patterns that could lead to a better understanding of TBIs. However, the amount of data structured and coded by the International Classification of Disease poses a challenge to its successful interpretation. The emerging field of data mining can be instrumental in helping to meet the daunting challenges faced by the TBI community. The report outlines novel areas for data mining relevant to TBI, and offers insight into how the above approach can be applied to solve pressing healthcare problems. Future work should focus on confirmatory analyses, which subsequently can guide precision medicine and preventive frameworks.