bluuebunny/crossref_metadata_2025_split huggingface dataset:
Five genomic regions have a major impact on fat composition in Iberian pigs
Authors: Pena R. N. , Noguera J. L. , García-Santana M. J. , González E. , Tejeda J. F. , Ros-Freixedes R. , Ibáñez-Escriche N. 
Published: February 2019

AbstractThe adipogenic nature of the Iberian pig defines many quality attributes of its fresh meat and dry-cured products. The distinct varieties of Iberian pig exhibit great variability in the genetic parameters for fat deposition and composition in muscle. The aim of this work is to identify common and distinct genomic regions related to fatty acid composition in Retinto, Torbiscal, and Entrepelado Iberian varieties and their reciprocal crosses through a diallelic experiment. In this study, we performed GWAS using a high density SNP array on 382 pigs with the multimarker regression Bayes B method implemented in GenSel. A number of genomic regions showed strong associations with the percentage of saturated and unsaturated fatty acid in intramuscular fat. In particular, five regions with Bayes Factor >100 (SSC2 and SSC7) or >50 (SSC2 and SSC12) explained an important fraction of the genetic variance for miristic, palmitoleic, monounsaturated (>14%), oleic (>10%) and polyunsaturated (>5%) fatty acids. Six genes (RXRB, PSMB8, CHGA, ACACA, PLIN4, PLIN5) located in these regions have been investigated in relation to intramuscular composition variability in Iberian pigs, with two SNPs at the RXRB gene giving the most consistent results on oleic and monounsaturated fatty acid content.
Combination of Platelet expression of PKCepsilon and cardiac troponin-I for early diagnosis of chest pain patients in the emergency department
Authors: Carubbi Cecilia , Masselli Elena , Pozzi Giulia , Mattioli Maria , Martini Silvia , Goldoni Matteo , Aloe Rosalia , Cervellin Gianfranco , Vitale Marco , Gobbi Giuliana 
Published: February 2019

AbstractA rapid differential diagnosis of the clinical conditions underlying chest pain is a relevant clinical issue. Specifically, a fast rule-in or -out of acute myocardial infarction (AMI) is mandatory to improve diagnostic outcome and cost-effectiveness of patient management. We demonstrated that Protein Kinase C (PKC) epsilon is selectively expressed by platelets from AMI patients, accounting for increased platelet activation. Thus, we hypothesized that PKCepsilon-expressing platelets may represent a pathophysiological marker of AMI that could be utilized in combination with troponin-I, the conventional marker of cardiac injury, to add diagnostic information in chest pain workup. In 94 chest pain patients consecutively admitted to Parma University Hospital, we tested the diagnostic performance of flow-cytometric detection of PKCepsilon expressing platelets in discriminating AMI vs. non-AMI conditions. We demonstrated that PKCepsilon-expressing platelets were significantly higher in patients with AMI. Flow cytometry detection of PKCepsilon-expressing platelets showed high sensitivity and specificity (87.5% and 84.4%, respectively) and good diagnostic accuracy (AUC: 0.875). The combination of PKCepsilon expressing platelets and cardiac troponin clearly discriminates patients with 100% and 0% of probability to be affected by AMI. Overall, we highlighted a dual marker strategy potentially useful for a rapid rule-in or -out of myocardial infarction in chest pain patients.
Juvenile hormone controls ovarian development in female Anopheles albimanus mosquitoes
Authors: Hernández-Martínez Salvador , Cardoso-Jaime Víctor , Nouzova Marcela , Michalkova Veronika , Ramirez Cesar E. , Fernandez-Lima Francisco , Noriega Fernando G. 
Published: February 2019

AbstractAnophelinae mosquitoes are vectors of human malaria, a disease that infects hundreds of millions of people and causes almost 600,000 fatalities annually. Despite their medical importance, laboratory studies on key aspects of Anophelinae reproductive biology have been limited, and in particular, relatively little is known about the role of juvenile hormone (JH) in the control of female reproduction. The study presented here attempts to fill a gap of knowledge in our understanding of the JH control of ovarian development in female Anophelinae mosquitoes, using Anopheles albimanus as a model. Our studies revealed that JH controls the tempo of maturation of primary follicles in An. albimanus in a similar manner to that previously described in Aedes aegypti. At adult eclosion JH hemolymph titer was low, increased in 1-day old sugar-fed insects, and decreased in blood fed individuals. JH titers decreased if An. albimanus females were starved, and were reduced if insects emerged with low teneral reserves, precluding previtellogenic ovarian development. However, absolute hemolymph titers were lower than Ae. aegypti. Decapitation experiments suggested that if teneral reserves are sufficient, factors from the head activate JH synthesis by the corpora allata (CA) during the first 9–12 h after adult emergence. In conclusion, our studies support the hypothesis that JH controls previtellogenic ovarian development in female An. albimanus mosquitoes, in a similar manner that have been described in Culicinae.
Simultaneous Improvement in the Strength and Formability of Commercially Pure Titanium via Twinning-induced Crystallographic Texture Control
Authors: Won Jong Woo , Park Chan Hee , Hong Jaekeun , Lee Chong Soo , Hong Seong-Gu 
Published: February 2019

AbstractThe rolling texture formed in the conventional cold rolling process of commercially pure titanium (CP-Ti) for producing a metal sheet significantly limits the potential applications of CP-Ti sheets in various industrial sectors by impairing the formability. Here, we report that by exploiting a twinning-induced crystallographic texture modification, the rolling texture can be weakened and dispersed effectively, leading to a simultaneous improvement in the formability and yield strength. A two-stage cold rolling process was designed with intermediate annealing at a late stage of the conventional cold rolling process to generate deformation twins. The intermediate annealing drove the activation of $$\{11\bar{2}2\}$$
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twin and $$\{11\bar{2}2\}$$
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– $$\{10\bar{1}2\}$$
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double twin in the second stage of the rolling process by removing the internal reaction stress developed in the first stage of the rolling process through recrystallization, and the crystallographic feature of the $$\{11\bar{2}2\}$$
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twinned region, i.e., $$\{11\bar{2}2\}$$
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twin texture, was effective for <a> type slips and $$\{10\bar{1}2\}$$
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twinning to accommodate a through-thickness strain as well as for reducing the planar anisotropy. This enhanced thinning capability and reduced planar anisotropy in the $$\{11\bar{2}2\}$$
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twin texture led to an improvement of the formability. We demonstrated the feasibility of the suggested two-stage cold rolling process with ASTM grade 2 CP-Ti.
Experimental proof of the reciprocal relation between spin Peltier and spin Seebeck effects in a bulk YIG/Pt bilayer
Authors: Sola Alessandro , Basso Vittorio , Kuepferling Michaela , Dubs Carsten , Pasquale Massimo 
Published: February 2019

AbstractWe verify for the first time the reciprocal relation between the spin Peltier and spin Seebeck effects in a bulk YIG/Pt bilayer. Both experiments are performed on the same YIG/Pt device by a setup able to accurately determine heat currents and to separate the spin Peltier heat from the Joule heat background. The sample-specific value for the characteristics of both effects measured on the present YIG/Pt bilayer is (6.2 ± 0.4) × 10−3 KA−1. In the paper we also discuss the relation of both effects with the intrinsic and extrinsic parameters of YIG and Pt and we envisage possible strategies to optimize spin Peltier refrigeration.
Three distinct regions of cRaf kinase domain interact with membrane
Authors: Prakash Priyanka , Hancock John F. , Gorfe Alemayehu A. 
Published: February 2019

AbstractRaf kinases are downstream effectors of small GTPase Ras. Mutations in Ras and Raf are associated with a variety of cancers and genetic disorders. Of the three Raf isoforms, cRaf is most frequently involved in tumor initiation by Ras. Cytosolic Raf is auto-inhibited and becomes active upon recruitment to the plasma membrane. Since the catalytic domain of Raf is its kinase domain, we ask the following: does the kinase domain of Raf has potential to interact with membrane and if yes, what role does the membrane interaction play? We present a model of cRaf kinase domain in complex with a heterogeneous membrane bilayer using atomistic molecular dynamics simulation. We show that the kinase domain of cRaf has three distinct membrane-interacting regions: a polybasic motif (R.RKTR) from the regulatory αC-helix, an aromatic/hydrophobic cluster from the N-terminal acidic region (NtA) and positively charged/aromatic cluster from the activation segment (AS). We show that residues from these regions form an extended membrane-interacting surface that resembles the membrane-interacting residues from known membrane-binding domains. Activating phosphorylatable regions (NtA and AS), make direct contact with the membrane whereas R.RKTR forms specific multivalent salt bridges with PA. PA lipids dwell for longer times around the R.RKTR motif. Our results suggest that membrane interaction of monomeric cRaf kinase domain likely orchestrates the Raf activation process and modulates its function. We show that R.RKTR is a hotspot that interacts with membrane when cRaf is monomeric and becomes part of the interface upon Raf dimerization. We propose that in terms of utilizing a specific hotspot to form membrane interaction and dimer formation, both Raf and its upstream binding partner KRas, are similar.
Structural, luminescence and thermometric properties of nanocrystalline YVO4:Dy3+ temperature and concentration series
Authors: Kolesnikov I. E. , Kalinichev A. A. , Kurochkin M. A. , Golyeva E. V. , Terentyeva A. S. , Kolesnikov E. Yu. , Lähderanta E. 
Published: February 2019

AbstractWe report systematic study of Dy3+-doped YVO4 nanophosphors synthesized via modified Pechini technique. Effect of calcination temperature and doping concentration on structure and luminescence has been investigated. XRD and Raman spectroscopy revealed preparation of single phase nanoparticles without any impurities. Synthesized nanopowders consisted of weakly agglomerated nanoparticles with average size about 50 nm. Photoluminescence spectra of YVO4:Dy3+ nanoparticles consisted of the characteristic narrow lines attributed to the intra-configurational 4f-4f transitions dominating by the hypersensitive 4F9/2–6H13/2 transition. The calcination temperature variation did not affect 4F9/2 lifetime, whereas increase of doping concentration resulted in its gradual decline. Potential application of YVO4:Dy3+ 1 at.% and 2 at.% nanopowders as ratiometric luminescence thermometers within 298–673 K temperature range was tested. The main performances of thermometer including absolute and relative thermal sensitivities and temperature uncertainty were calculated. The maximum relative thermal sensitivity was determined to be 1.8% K−1@298 K, whereas the minimum temperature uncertainty was 2 K.
Reflection of treatment proficiency of hydroxyurea treated β-thalassemia serum samples through nuclear magnetic resonance based metabonomics
Authors: Khalid Ayesha , Siddiqui Amna Jabbar , Ansari Saqib Hussain , Musharraf Syed Ghulam 
Published: February 2019

Abstractβ-Thalassemia is a widespread autosomal recessive blood disorder found in most parts of the world. Fetal hemoglobin (HbF), a form of hemoglobin is found in infants, replaced by adult hemoglobin (HbA) after birth. Hydroxyurea (HU) is one of the most effective HbF inducer used for the treatment of anemic diseases. We aimed to improve the understanding of HU therapy in β-thalassemia by metabonomics approach using 1H NMR spectroscopy. This study includes 40 cases of β-thalassemia before and after HU therapy along with 40 healthy as controls. Carr-Purcell-Meiboom-Gill (CPMG) sequence was used to identify forty-one putative metabolites. Generation of models like partial least square discriminant analysis (PLS-DA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) based on different metabolites including lipids, amino acids, glucose, fucose, isobutyrate, and glycerol revealed satisfactory outcomes with 85.2% and 91.1% classification rates, respectively. The concentration of these metabolites was altered in β-thalassemia samples. However, after HU treatment metabolic profile of same patients showed closeness towards healthy. Deviant metabolic pathways counting lipoprotein changes, glycolysis, TCA cycle, fatty acid and choline metabolisms were identified as having significant differences among study groups. Findings of this study may open a better way to monitor HU treatment effectiveness in β-thalassemia patients, as the results suggested that metabolic profile of β-thalassemia patients shows similarity towards normal profile after this therapy.
Sonication induced amorphisation in Ag nanowires
Authors: Dai Han , Li Haitao , Li Zhutie , Zhao Junfeng , Yu Xinxiang , Sun Jie , An Qi 
Published: February 2019

AbstractIt has long been conjectured that pure-element face-centred cubic (fcc) metals can be transformed into a glassy state by deformation at ultra-high strain rates. However, when an impact force is applied at the nanoscale, deformation-induced melting prevents observations of fcc metal amorphisation. Here we propose a sonication treatment of Ag nanowires (fcc) and confirmed amorphisation induced by high strain rates at bent areas of the Ag nanowires. Owing to the mismatch of the deformation modes between the core and the surface, we observed a diameter related increase of the ductility of Ag nanowires under deformation at ultra-high strain rates generated by sonication. The sonication-prepared amorphous Ag was stable at room temperature. Amorphous Ag at the bent areas was highly reactive and was readily recrystallized under light illumination or vulcanised. Our study verifies the occurrence of high strain rate induced amorphisation in pure fcc MGs and provides a powerful tool for mechanical studies on metal nanomaterials under extremely high strain rates and forces.
Synthesis and Characterization of Multiple-Cation Rb(MAFA)PbI3 Perovskite Single Crystals
Authors: Kim Hyojung , Byun Hye Ryung , Jeong Mun Seok 
Published: February 2019

AbstractWe synthesized multiple-cation Rb(MAFA)PbI3 perovskite single crystals for the first time. The effect of Rb+ substitution was systemically investigated, and the addition of 1.5 M 5% RbI was the optimum condition to obtain high-quality Rb(MAFA)PbI3 single crystals. Lattice shrinkage occurred in the Rb(MAFA)PbI3 single crystal because of the small ionic radius of Rb+, resulting in blue-shifted absorption and photoluminescence (PL) peaks. The 1.5 M 5% RbI-added (MAFA)PbI3 single crystal showed the longest carrier lifetime of 18.35 ns, exhibiting the highest photoresponse than other crystals. We believe that this work will provide a basic insight into the mixed-cation perovskite single crystals for the future optoelectronic applications.
Distinct Metabolic Endotype Mirroring Acute Respiratory Distress Syndrome (ARDS) Subphenotype and its Heterogeneous Biology
Authors: Viswan Akhila , Ghosh Pralay , Gupta Devendra , Azim Afzal , Sinha Neeraj 
Published: February 2019

AbstractPredisposing aetiologies in Acute Respiratory Distress Syndrome (ARDS), perpetuates to heterogeneous clinical course hampering therapeutic response. Therefore, physiological variables need to be identified by stratifying ARDS subphenotypes and endotype, to target ARDS heterogeneity. The present study is stimulated by the fact that the ARDS heterogeneity arises from diverse pathophysiological changes leading to distinct ARDS endotypes characterized by perturbed biological mechanism which can be exploited in terms of metabolic profile by metabolomics. Biological endotypes using (n = 464 patients and controls), mBALF and serum samples were identified by high – resolution NMR spectroscopy from two clinically diagnosed ARDS subtypes grouped under mild, moderate and severe ARDS as subphenotype1and pulmonary and extra – pulmonary ARDS as subphenotype2. The identified mBALF endotypes (isoleucine, leucine, valine, lysine/arginine, tyrosine, threonine) and serum endotypes (proline, glutamate, phenylalanine, valine) in both subphenotypes by statistical analysis were tested for their reproducibility and robustness. By combining metabolic endotypes with clinical based mortality score (APACHE and SOFA) added to their predictive performance as ARDS mortality predictors. Thus, a comprehensive set of mBALF endotypes representing compartmentalized lung milieu and serological endotypes representing systemic markers of ARDS subtypes were validated. The interlinked biological pathway of these disease specific endotype further elucidated their role as candidate biomarker in governing ARDS heterogeneous biology.
Extremely robust photocurrent generation of titanium dioxide photoanodes bio-sensitized with recombinant microalgal light-harvesting proteins
Authors: Lämmermann Nina , Schmid-Michels Fabian , Weißmann Aike , Wobbe Lutz , Hütten Andreas , Kruse Olaf 
Published: February 2019

AbstractBio-dyes for light harvesting in dye-sensitized solar cells (DSSC) have the advantage of being environmentally-friendly, non-toxic alternatives, which can be produced in a sustainable fashion. Free photosynthetic pigments are unstable in the presence of light and oxygen, a situation which can hardly be avoided during the operation of DSSCs, especially in large-scale applications. We therefore investigated the recombinant light-harvesting protein LHCBM6, which naturally occurs in the photosynthetic apparatus of the green microalga Chlamydomonas reinhardtii as a bio-dye in DSSCs. Photocurrent densities of up to 0.87 and 0.94 mA·cm−2 were determined for the DSSCs and solar energy to electricity conversion efficiencies (η) reached about 0.3% (100 mW·cm−2; AM 1.5 G filter applied). Importantly, we observed an unprecedented stability of LHCII-based DSSCs within long DSSC operation times of at least 7 days in continuous light and show that operation times are restricted by electrolyte decomposition rather than reduced dye performance, as could be demonstrated by DSSC reactivation following re-supplementation with fresh electrolyte. To the best of our knowledge, this is the first study analysing bio-dye sensitized DSSCs over such long periods, which revealed that during illumination an activation of the DSSCs occurs.
Overlapping and differential functions of ATF6α versus ATF6β in the mouse heart
Authors: Correll Robert N. , Grimes Kelly M. , Prasad Vikram , Lynch Jeffrey M. , Khalil Hadi , Molkentin Jeffery D. 
Published: February 2019

AbstractHemodynamic stress on the mammalian heart results in compensatory hypertrophy and activation of the unfolded protein response through activating transcription factor 6α (ATF6α) in cardiac myocytes, but the roles of ATF6α or the related transcription factor ATF6β in regulating this hypertrophic response are not well-understood. Here we examined the effects of loss of ATF6α or ATF6β on the cardiac response to pressure overload. Mice gene-deleted for Atf6 or Atf6b were subjected to 2 weeks of transverse aortic constriction, and each showed a significant reduction in hypertrophy with reduced expression of endoplasmic reticulum (ER) stress-associated proteins compared with controls. However, with long-term pressure overload both Atf6 and Atf6b null mice showed enhanced decompensation typified by increased heart weight, pulmonary edema and reduced function compared to control mice. Our subsequent studies using cardiac-specific transgenic mice expressing the transcriptionally active N-terminus of ATF6α or ATF6β revealed that these factors control overlapping gene expression networks that include numerous ER protein chaperones and ER associated degradation components. This work reveals previously unappreciated roles for ATF6α and ATF6β in regulating the pressure overload induced cardiac hypertrophic response and in controlling the expression of genes that condition the ER during hemodynamic stress.
Selective Capture and Purification of MicroRNAs and Intracellular Proteins through Antisense-vectorized Magnetic Nanobeads
Authors: Gessner Isabel , Yu Xiaojie , Jüngst Christian , Klimpel Annika , Wang Lingyu , Fischer Thomas , Neundorf Ines , Schauss Astrid C. , Odenthal Margarete , Mathur Sanjay 
Published: February 2019

AbstractMicroRNAs (miRNAs) are small non-coding nucleotides playing a crucial role in posttranscriptional expression and regulation of target genes in nearly all kinds of cells. In this study, we demonstrate a reliable and efficient capture and purification of miRNAs and intracellular proteins using magnetic nanoparticles functionalized with antisense oligonucleotides. For this purpose, a tumor suppressor miRNA (miR-198), deregulated in several human cancer types, was chosen as the model oligonucleotide. Magnetite nanoparticles carrying the complementary sequence of miR-198 (miR-198 antisense) on their surface were delivered into cells and subsequently used for the extracellular transport of miRNA and proteins. The successful capture of miR-198 was demonstrated by isolating RNA from magnetic nanoparticles followed by real-time PCR quantification. Our experimental data showed that antisense-coated particles captured 5-fold higher amounts of miR-198 when compared to the control nanoparticles. Moreover, several proteins that could play a significant role in miR-198 biogenesis were found attached to miR-198 conjugated nanoparticles and analyzed by mass spectrometry. Our findings demonstrate that a purpose-driven vectorization of magnetic nanobeads with target-specific recognition ligands is highly efficient in selectively transporting miRNA and disease-relevant proteins out of cells and could become a reliable and useful tool for future diagnostic, therapeutic and analytical applications.
CaMello-XR enables visualization and optogenetic control of Gq/11 signals and receptor trafficking in GPCR-specific domains
Authors: Eickelbeck Dennis , Karapinar Raziye , Jack Alexander , Suess Sandra T. , Barzan Ruxandra , Azimi Zohre , Surdin Tatjana , Grömmke Michelle , Mark Melanie D. , Gerwert Klaus , Jancke Dirk , Wahle Petra , Spoida Katharina , Herlitze Stefan 
Published: February 2019

AbstractThe signal specificity of G protein-coupled receptors (GPCRs) including serotonin receptors (5-HT-R) depends on the trafficking and localization of the GPCR within its subcellular signaling domain. Visualizing traffic-dependent GPCR signals in neurons is difficult, but important to understand the contribution of GPCRs to synaptic plasticity. We engineered CaMello (Ca2+-melanopsin-local-sensor) and CaMello-5HT2A for visualization of traffic-dependent Ca2+ signals in 5-HT2A-R domains. These constructs consist of the light-activated Gq/11 coupled melanopsin, mCherry and GCaMP6m for visualization of Ca2+ signals and receptor trafficking, and the 5-HT2A C-terminus for targeting into 5-HT2A-R domains. We show that the specific localization of the GPCR to its receptor domain drastically alters the dynamics and localization of the intracellular Ca2+ signals in different neuronal populations in vitro and in vivo. The CaMello method may be extended to every GPCR coupling to the Gq/11 pathway to help unravel new receptor-specific functions in respect to synaptic plasticity and GPCR localization.