bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of gaseous allyl isothiocyanate against the growth of mycotoxigenic fungi and mycotoxin production in corn stored for 6 months
Authors: Nazareth Tiago M , Corrêa Jessica A F , Pinto Anne C S M , Palma Juliano B , Meca Giuseppe , Bordin Keliani , Luciano Fernando B 
Published: April 2018

AbstractBACKGROUNDBrazil produces approximately 63 million tons of corn kernels annually, which is commonly contaminated with fungi and mycotoxins. The objective of this study was to evaluate the efficacy of gaseous allyl isothiocyanate (AITC) to inhibit the growth of Aspergillus parasiticus and Fusarium verticillioides, and mycotoxin production (aflatoxins B1, B2, G1 and G2, fumonisins B1 and B2) in corn during 180 days of storage.RESULTSAITC at 50 µL L−1 resulted in a significant reduction of the fungal population (P < 0.05) after 180 days, decreasing 3.17 log(CFU g−1) and 3.9 log(CFU g−1) of A. parasiticus and F. verticillioides respectively in comparison with the control. In addition, 10 and 50 µL L−1 treatments prevented the production of fumonisin B1 for the whole period. Aflatoxins were not detected in either control or treated groups. Residual levels of AITC in corn treated with 10 µL L−1 and 50 µL L−1 were detected up to 14 days and 30 days respectively.CONCLUSIONProphylactic treatment with AITC reduced the fungal population and inhibited fumonisin B1 production in stored corn, exhibiting great potential to be applied in corn silos to prevent fungi contamination and minimize mycotoxin levels. © 2018 Society of Chemical Industry
Effect of thermal processing towards lipid oxidation and non‐enzymatic browning reactions of Antarctic krill (Euphausia superba) meal
Authors: Liu Yanzi , Cong Peixu , Li Beijia , Song Yu , Liu Yanjun , Xu Jie , Xue Changhu 
Published: April 2018

AbstractBACKGROUNDAntarctic krill is a huge source of biomass and prospective high‐quality lipid source. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), nutritionally important lipid components with poor oxidative stability, were used as markers of oxidation during thermal processing of Antarctic krill (Euphausia superba) meal by evaluating the lipolysis, lipid oxidation, and non‐enzymatic browning reactions.RESULTLiquid chromatography–mass spectrometry of the phospholipids and the main oxidation products of free fatty acids and phosphatidylcholine (PC) was effective for evaluating the oxidation of EPA and DHA. During boiling, oxidation of EPA and DHA in the free fatty acid and PC fractions and hydrolysis of the fatty acids at the sn‐2 position of the phospholipids were predominant. The changes in PC during drying were mainly attributed to the oxidation of EPA and DHA. Heat treatment increased the oxidation products and concentration of hydrophobic pyrrole owing to pyrrolization between phosphatidylethanolamine and the lipid oxidation products.CONCLUSIONThe lipid oxidation level of Antarctic krill increased after drying, owing to prolonged heating under the severe conditions. © 2018 Society of Chemical Industry
Quick changes of milk fatty acids after inclusion or suppression of linseed oil in the diet of goats
Authors: Gómez‐Cortés Pilar , Cívico Alfonso , de la Fuente Miguel Angel , Núñez Sánchez Nieves , Juárez Manuela , Peña Blanco Francisco , Martínez Marín Andrés L 
Published: April 2018

AbstractBACKGROUNDLipid supplementation of ruminant diet is an excellent tool to improve the nutritional quality of dairy fat. The purpose of this research was to monitor in detail the goat milk fatty acid (FA) profile during the first 24 h after linseed oil (LO) supplementation or suppression in the diet. Particular emphasis was placed in the changes of FA with bioactive properties. Milk fat was analysed by gas chromatography from milkings at 0, 1, 3, 6, 12 and 24 h after diet shift.RESULTSThe α‐linolenic acid levels increased 12 h after LO incorporation in the diet and decreased 3 h after oil suppression. Most of the milk 10:0 to 16:0 saturated FA decreased 24 h after LO supplementation, whereas oil suppression raised their levels after 6 h. Similarly, raising of mono‐ and polyunsaturated trans‐FA after LO inclusion was delayed in comparison with their decrease after oil suppression.CONCLUSIONThis study supports that ruminal bacteria and mammary glands would exhibit a fast responsiveness after the inclusion or suppression of LO in ruminant rations. Milk with an improved FA profile could be collected between 12 h after LO supplementation and the last milking before LO suppression in the diet. © 2018 Society of Chemical Industry
Minimizing Post‐operative Complications of Groin Dissection Using Modified Skin Bridge Technique: A Single‐Centre Descriptive Study Showing Post‐operative and Early Oncological Outcomes
Authors: Ray Mukur Dipi , Jakhetiya Ashish , Kumar Sunil , Mishra Ashutosh , Singh Seema , Shukla Nootan Kumar 
Published: April 2018

AbstractIntroductionHistorically, groin dissections are associated with high morbidity. Various modifications have been described in the literature with inconsistent outcomes. The aim of this paper is to highlight modified skin bridge technique to minimize all post‐operative complications of groin dissection without compromising early oncological outcomes.MethodsA retrospective descriptive study of the computerized cancer database was performed to retrieve details of all the cancer patients who had undergone groin dissections during January 2012 to September 2016. Data pertaining to clinical profile including demographics, clinical and histopathological details, treatment profile, procedure‐related morbidity and relapse patterns were extracted and analysed.ResultsA total of 75 patients underwent 105 groin dissections during this period. Out of 105 groin dissections, 43 were inguinal lymph node dissection (ILND) and 62 were combined ilio‐inguinal lymph node dissection (IILND). The most common diagnosis was carcinoma penis (25%) followed by malignant melanoma (14.6%) and squamous cell carcinoma (13.33%) of lower extremities. Overall, the most common complications were seroma (14.28%) and skin edge necrosis (7.61%) followed by surgical site infection (4.76%). After a median follow‐up of 17.64 months (IQR 5–61.53), a total of 18 patients (24%) developed recurrence.ConclusionGroin dissection still remains an important diagnostic as well as therapeutic procedure justifying its potential of morbidity. Modified skin bridge technique is a very effective method to minimize all post‐operative complications with optimal early oncological outcomes.
Gender Differences in the Consequences of Divorce: A Study of Multiple Outcomes
Authors: Leopold Thomas 
Published: April 2018

AbstractIn this study, I examined gender differences in the consequences of divorce by tracing annual change in 20 outcome measures covering four domains: economic, housing and domestic, health and well-being, and social. I used data from the German Socio-Economic Panel Study (SOEP) and fixed-effects panel regression models on a sample of N = 18,030 individuals initially observed in a marital union, N = 1,220 of whom divorced across the observation period (1984–2015). Three main findings emerged from the analysis. First, men were more vulnerable to short-term consequences of divorce for subjective measures of well-being, but postdivorce adaptation alleviated gender differences in these outcomes. Second, a medium-term view on multiple outcomes showed more similarity than differences between women and men. The medium-term consequences of divorce were similar in terms of subjective economic well-being; mental health, physical health, and psychological well-being; residential moves, homeownership, and satisfaction with housework; and chances of repartnering, social integration with friends and relatives, and feelings of loneliness. Third, the key domain in which large and persistent gender differences emerged were women’s disproportionate losses in household income and associated increases in their risk of poverty and single parenting. Taken together, these findings suggest that men’s disproportionate strain of divorce is transient, whereas women’s is chronic.
Pre‐treatment with proton pump inhibitors decreases the success of primary Helicobacter pylori eradication using a vonoprazan‐based regimen
Authors: Shinozaki Satoshi , Osawa Hiroyuki , Sakamoto Hirotsugu , Hayashi Yoshikazu , Kobayashi Yasutoshi , Miura Yoshimasa , Lefor Alan Kawarai , Yamamoto Hironori 
Published: April 2018

AbstractVonoprazan‐based regimens have improved the rate of successful Helicobacter pylori (H. pylori) eradication, but it has not reached 100%. The aim of this study is to clarify significant predictors of successful H. pylori eradication using a vonoprazan‐based regimen. In this retrospective cohort study, 174 patients who underwent primary H. pylori eradication therapy were included. All patients underwent esophagogastroduodenoscopy before treatment. The vonoprazan‐based regimen includes amoxicillin 750 mg, clarithromycin 200 mg and vonoprazan 20 mg twice daily for one week. Pre‐treatment with a proton pump inhibitor (PPI) was defined as continued PPI use for more than four weeks prior to eradication therapy. The rates of successful eradication were 83% (145/174) in intention‐to‐treat analysis and 85% (145/171) in per‐protocol analysis. Predictors of successful eradication among 171 patients were evaluated in per‐protocol analysis. In univariate analysis, male gender was a significant positive predictor of successful eradication (odds ratio [OR] 3.813, 95% confidence interval [CI] 1.363–10.663, p = 0.010) and pre‐treatment with PPIs was a negative predictor (OR 0.193, 95%CI 0.076–0.485, p < 0.001). In multivariate analysis, male gender remained a positive predictor (OR 3.826, 95%CI 1.317–11.116, p = 0.013), and pre‐treatment with PPIs (OR 0.232, 95%CI 0.087–0.615, p = 0.003) remained a negative predictor. In conclusion, pre‐treatment with PPIs before eradication therapy decreases the rate of successful eradication. Therefore, it may be desirable to discontinue pre‐treatment with PPIs prior to eradication therapy, because of the potential to improve the rate of successful eradication.
Live music network in Larnaca, Cyprus: from musicians to audience and back (to the venue) again
Authors: Kouvarou Maria 
Published: April 2018

AbstractThis article discusses the development of a live music network in Larnaca, Cyprus. It is the result of a 9 month field research project centred on a specific music venue and it is based on interviews with members of four local prominent rock bands and the venue manager. These interviews were further informed by several discussions with numerous audience members, the result of the author's participatory involvement in this network. The emphasis of the discussion lies in the experiences of the performers and their views concerning the growth of this live music network, which in itself is an indicator of the dynamic relationship between underground musical tastes and the presence of mainstream currents in the city. The purpose of this research is to highlight that the development and preservation of such live music networks depend on various factors and to argue that the study of transformations of a locality's musical life can lead to sociological readings regarding its music-related past and present.
Excitatory and inhibitory synaptic dysfunction in mania: an emerging hypothesis from animal model studies
Authors: Lee Yeunkum , Zhang Yinhua , Kim Shinhyun , Han Kihoon 
Published: April 2018

AbstractBipolar disorder (BD) is a common psychiatric disorder characterized by recurrent mood swings between depression and mania, and is associated with high treatment costs. The existence of manic episodes is the defining feature of BD, during which period, patients experience extreme elevation in activity, energy, and mood, with changes in sleep patterns that together severely impair their ability to function in daily life. Despite some limitations in recapitulating the complex features of human disease, several rodent models of mania have been generated and characterized, which have provided important insights toward understanding its underlying pathogenic mechanisms. Among the mechanisms, neuronal excitatory and inhibitory (E/I) synaptic dysfunction in some brain regions, including the frontal cortex, hippocampus, and striatum, is an emerging hypothesis explaining mania. In this review, we highlight recent studies of rodent manic models having impairments in the E/I synaptic development and function. We also summarize the molecular and functional changes of E/I synapses by some mood stabilizers that may contribute to the therapeutic efficacy of drugs. Furthermore, we discuss potential future directions in the study of this emerging hypothesis to better connect the outcomes of basic research to the treatment of patients with this devastating mental illness.
Olfactory marker protein regulates prolactin secretion and production by modulating Ca2+ and TRH signaling in lactotrophs
Authors: Kang Chan Woo , Han Ye Eon , Lee Mi Kyung , Cho Yoon Hee , Kang NaNa , Koo JaeHyung , Ku Cheol Ryong , Lee Eun Jig 
Published: April 2018

AbstractOlfactory marker protein (OMP) is a marker of olfactory receptor-mediated chemoreception, even outside the olfactory system. Here, we report that OMP expression in the pituitary gland plays a role in basal and thyrotropin-releasing hormone (TRH)-induced prolactin (PRL) production and secretion. We found that OMP was expressed in human and rodent pituitary glands, especially in PRL-secreting lactotrophs. OMP knockdown in GH4 rat pituitary cells increased PRL production and secretion via extracellular signal-regulated kinase (ERK)1/2 signaling. Real-time PCR analysis and the Ca2+ influx assay revealed that OMP was critical for TRH-induced PRL secretion. OMP-knockout mice showed lower fertility than control mice, which was associated with increased basal PRL production via activation of ERK1/2 signaling and reduced TRH-induced PRL secretion. However, both in vitro and in vivo results indicated that OMP was only required for hormone production and secretion because ERK1/2 activation failed to stimulate cell proliferation. Additionally, patients with prolactinoma lacked OMP expression in tumor tissues with hyperactivated ERK1/2 signaling. These findings indicate that OMP plays a role in PRL production and secretion in lactotrophs through the modulation of Ca2+ and TRH signaling.
Super enhancer inhibitors suppress MYC driven transcriptional amplification and tumor progression in osteosarcoma
Authors: Chen Demeng , Zhao Zhiqiang , Huang Zixin , Chen Du-Chu , Zhu Xin-Xing , Wang Yi-Ze , Yan Ya-Wei , Tang Shaojun , Madhavan Subha , Ni Weiyi , Huang Zhan-peng , Li Wen , Ji Weidong , Shen Huangxuan , Lin Shuibin , Jiang Yi-Zhou 
Published: April 2018

AbstractOsteosarcoma is the most common primary bone sarcoma that mostly occurs in young adults. The causes of osteosarcoma are heterogeneous and still not fully understood. Identification of novel, important oncogenic factors in osteosarcoma and development of better, effective therapeutic approaches are in urgent need for better treatment of osteosarcoma patients. In this study, we uncovered that the oncogene MYC is significantly upregulated in metastastic osteosarcoma samples. In addition, high MYC expression is associated with poor survival of osteosarcoma patients. Analysis of MYC targets in osteosarcoma revealed that most of the osteosarcoma super enhancer genes are bound by MYC. Treatment of osteosarcoma cells with super enhancer inhibitors THZ1 and JQ1 effectively suppresses the proliferation, migration, and invasion of osteosarcoma cells. Mechanistically, THZ1 treatment suppresses a large group of super enhancer containing MYC target genes including CDK6 and TGFB2. These findings revealed that the MYC-driven super enhancer signaling is crucial for the osteosarcoma tumorigenesis and targeting the MYC/super enhancer axis represents as a promising therapeutic strategy for treatment of osteosarcoma patients.
A proteomics landscape of circadian clock in mouse liver
Authors: Wang Yunzhi , Song Lei , Liu Mingwei , Ge Rui , Zhou Quan , Liu Wanlin , Li Ruiyang , Qie Jingbo , Zhen Bei , Wang Yi , He Fuchu , Qin Jun , Ding Chen 
Published: April 2018

AbstractAs a circadian organ, liver executes diverse functions in different phase of the circadian clock. This process is believed to be driven by a transcription program. Here, we present a transcription factor (TF) DNA-binding activity-centered multi-dimensional proteomics landscape of the mouse liver, which includes DNA-binding profiles of different TFs, phosphorylation, and ubiquitylation patterns, the nuclear sub-proteome, the whole proteome as well as the transcriptome, to portray the hierarchical circadian clock network of this tissue. The TF DNA-binding activity indicates diurnal oscillation in four major pathways, namely the immune response, glucose metabolism, fatty acid metabolism, and the cell cycle. We also isolate the mouse liver Kupffer cells and measure their proteomes during the circadian cycle to reveal a cell-type resolved circadian clock. These comprehensive data sets provide a rich data resource for the understanding of mouse hepatic physiology around the circadian clock.
Evolutionary plasticity of the NHL domain underlies distinct solutions to RNA recognition
Authors: Kumari Pooja , Aeschimann Florian , Gaidatzis Dimos , Keusch Jeremy J. , Ghosh Pritha , Neagu Anca , Pachulska-Wieczorek Katarzyna , Bujnicki Janusz M. , Gut Heinz , Großhans Helge , Ciosk Rafal 
Published: April 2018

AbstractRNA-binding proteins regulate all aspects of RNA metabolism. Their association with RNA is mediated by RNA-binding domains, of which many remain uncharacterized. A recently reported example is the NHL domain, found in prominent regulators of cellular plasticity like the C. elegans LIN-41. Here we employ an integrative approach to dissect the RNA specificity of LIN-41. Using computational analysis, structural biology, and in vivo studies in worms and human cells, we find that a positively charged pocket, specific to the NHL domain of LIN-41 and its homologs (collectively LIN41), recognizes a stem-loop RNA element, whose shape determines the binding specificity. Surprisingly, the mechanism of RNA recognition by LIN41 is drastically different from that of its more distant relative, the fly Brat. Our phylogenetic analysis suggests that this reflects a rapid evolution of the domain, presenting an interesting example of a conserved protein fold that acquired completely different solutions to RNA recognition.
Aggressive natural killer-cell leukemia mutational landscape and drug profiling highlight JAK-STAT signaling as therapeutic target
Authors: Dufva Olli , Kankainen Matti , Kelkka Tiina , Sekiguchi Nodoka , Awad Shady Adnan , Eldfors Samuli , Yadav Bhagwan , Kuusanmäki Heikki , Malani Disha , Andersson Emma I , Pietarinen Paavo , Saikko Leena , Kovanen Panu E. , Ojala Teija , Lee Dean A. , Loughran Thomas P. , Nakazawa Hideyuki , Suzumiya Junji , Suzuki Ritsuro , Ko Young Hyeh , Kim Won Seog , Chuang Shih-Sung , Aittokallio Tero , Chan Wing C. , Ohshima Koichi , Ishida Fumihiro , Mustjoki Satu 
Published: April 2018

AbstractAggressive natural killer-cell (NK-cell) leukemia (ANKL) is an extremely aggressive malignancy with dismal prognosis and lack of targeted therapies. Here, we elucidate the molecular pathogenesis of ANKL using a combination of genomic and drug sensitivity profiling. We study 14 ANKL patients using whole-exome sequencing (WES) and identify mutations inSTAT3(21%) and RAS-MAPK pathway genes (21%) as well as inDDX3X(29%) and epigenetic modifiers (50%). Additional alterations include JAK-STAT copy gains and tyrosine phosphatase mutations, which we show recurrent also in extranodal NK/T-cell lymphoma, nasal type (NKTCL) through integration of public genomic data. Drug sensitivity profiling further demonstrates the role of the JAK-STAT pathway in the pathogenesis of NK-cell malignancies, identifying NK cells to be highly sensitive to JAK and BCL2 inhibition compared to other hematopoietic cell lineages. Our results provide insight into ANKL genetics and a framework for application of targeted therapies in NK-cell malignancies.
Reduced oxidative capacity in macrophages results in systemic insulin resistance
Authors: Jung Saet-Byel , Choi Min Jeong , Ryu Dongryeol , Yi Hyon-Seung , Lee Seong Eun , Chang Joon Young , Chung Hyo Kyun , Kim Yong Kyung , Kang Seul Gi , Lee Ju Hee , Kim Koon Soon , Kim Hyun Jin , Kim Cuk-Seong , Lee Chul-Ho , Williams Robert W. , Kim Hail , Lee Heung Kyu , Auwerx Johan , Shong Minho 
Published: April 2018

AbstractOxidative functions of adipose tissue macrophages control the polarization of M1-like and M2-like phenotypes, but whether reduced macrophage oxidative function causes systemic insulin resistance in vivo is not clear. Here, we show that mice with reduced mitochondrial oxidative phosphorylation (OxPhos) due to myeloid-specific deletion of CR6-interacting factor 1 (Crif1), an essential mitoribosomal factor involved in biogenesis of OxPhos subunits, have M1-like polarization of macrophages and systemic insulin resistance with adipose inflammation. Macrophage GDF15 expression is reduced in mice with impaired oxidative function, but induced upon stimulation with rosiglitazone and IL-4. GDF15 upregulates the oxidative function of macrophages, leading to M2-like polarization, and reverses insulin resistance in ob/ob mice and HFD-fed mice with myeloid-specific deletion of Crif1. Thus, reduced macrophage oxidative function controls systemic insulin resistance and adipose inflammation, which can be reversed with GDF15 and leads to improved oxidative function of macrophages.
Design and optimization of a compact laser-driven proton beamline
Authors: Scisciò M. , Migliorati M. , Palumbo L. , Antici P. 
Published: April 2018

AbstractLaser-accelerated protons, generated by irradiating a solid target with a short, energetic laser pulse at high intensity (I > 1018 W·cm−2), represent a complementary if not outperforming source compared to conventional accelerators, due to their intrinsic features, such as high beam charge and short bunch duration. However, the broadband energy spectrum of these proton sources is a bottleneck that precludes their use in applications requiring a more reduced energy spread. Consequently, in recent times strong effort has been put to overcome these limits and to develop laser-driven proton beamlines with low energy spread. In this paper, we report on beam dynamics simulations aiming at optimizing a laser-driven beamline - i.e. a laser-based proton source coupled to conventional magnetic beam manipulation devices - producing protons with a reduced energy spread, usable for applications. The energy range of investigation goes from 2 to 20 MeV, i.e. the typical proton energies that can be routinely obtained using commercial TW-power class laser systems. Our beamline design is capable of reducing the energy spread below 20%, still keeping the overall transmission efficiency around 1% and producing a proton spot-size in the range of 10 mm2. We briefly discuss the results in the context of applications in the domain of Cultural Heritage.