bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non‐pharmacological management of persistent headaches associated with neck pain: A clinical practice guideline from the Ontario protocol for traffic injury management (OPTIMa) collaboration
Authors: Côté Pierre , Yu Hainan , Shearer Heather M. , Randhawa Kristi , Wong Jessica J. , Mior Silvano , Ameis Arthur , Carroll Linda J. , Nordin Margareta , Varatharajan Sharanya , Sutton Deborah , Southerst Danielle , Jacobs Craig , Stupar Maja , Taylor‐Vaisey Anne , Gross Douglas P. , Brison Robert J. , Paulden Mike , Ammendolia Carlo , Cassidy J. David , Loisel Patrick , Marshall Shawn , Bohay Richard N. , Stapleton John , Lacerte Michel 
Published: February 2019

AbstractObjectivesTo develop an evidence‐based guideline for the non‐pharmacological management of persistent headaches associated with neck pain (i.e., tension‐type or cervicogenic).MethodsThis guideline is based on systematic reviews of high‐quality studies. A multidisciplinary expert panel considered the evidence of clinical benefits, cost‐effectiveness, societal and ethical values, and patient experiences when formulating recommendations. Target audience includes clinicians; target population is adults with persistent headaches associated with neck pain.ResultsWhen managing patients with headaches associated with neck pain, clinicians should (a) rule out major structural or other pathologies, or migraine as the cause of headaches; (b) classify headaches associated with neck pain as tension‐type headache or cervicogenic headache once other sources of headache pathology has been ruled out; (c) provide care in partnership with the patient and involve the patient in care planning and decision making; (d) provide care in addition to structured patient education; (e) consider low‐load endurance craniocervical and cervicoscapular exercises for tension‐type headaches (episodic or chronic) or cervicogenic headaches >3 months duration; (f) consider general exercise, multimodal care (spinal mobilization, craniocervical exercise and postural correction) or clinical massage for chronic tension‐type headaches; (g) do not offer manipulation of the cervical spine as the sole form of treatment for episodic or chronic tension‐type headaches; (h) consider manual therapy (manipulation with or without mobilization) to the cervical and thoracic spine for cervicogenic headaches >3 months duration. However, there is no added benefit in combining spinal manipulation, spinal mobilization and exercises; and (i) reassess the patient at every visit to assess outcomes and determine whether a referral is indicated.ConclusionsOur evidence‐based guideline provides recommendations for the conservative management of persistent headaches associated with neck pain. The impact of the guideline in clinical practice requires validation.SignificanceNeck pain and headaches are very common comorbidities in the population. Tension‐type and cervicogenic headaches can be treated effectively with specific exercises. Manual therapy can be considered as an adjunct therapy to exercise to treat patients with cervicogenic headaches. The management of tension‐type and cervicogenic headaches should be patient‐centred.
Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes
Authors: Cottet Jonathan , Kehren Alexandre , Lasli Soufian , van Lintel Harald , Buret François , Frénéa‐Robin Marie , Renaud Philippe 
Published: February 2019

AbstractWe present a microfluidic platform allowing dielectrophoresis‐assisted formation of cell aggregates of controlled size and composition under flow conditions. When specific experimental conditions are met, negative dielectrophoresis allows efficient concentration of cells towards electric field minima and subsequent aggregation. This bottom‐up assembly strategy offers several advantages with respect to the targeted application: first, dielectrophoresis offers precise control of spatial cell organization, which can be adjusted by optimizing electrode design. Then, it could contribute to accelerate the establishment of cell‐cell interactions by favoring close contact between neighboring cells. The trapping geometry of our chip is composed of eight electrodes arranged in a circle. Several parameters have been tested in simulations to find the best configurations for trapping in flow. Those configurations have been tested experimentally with both polystyrene beads and human embryonic kidney cells. The final design and experimental setup have been optimized to trap cells and release the created aggregates on demand.
Modified carbon‐dioxide measurement to predict the heat release rate of fire burning in a compartment based on the three‐zone model
Authors: Zhang Ying , Tang Kaixuan , Duan Hong , Niu Yi , Huang Xianjia , Chen Bing , Liu Zhiyang , Chen Yue 
Published: February 2019

SummaryThe heat release rate (HRR) of fuels has been described as the single important variable of fuels in fire hazard, and the HRR experimental measurement remains a key issue in fire science. A modified carbon‐dioxide generation (CDG) method, applying a three‐zone smoke model, is developed to predict the HRR of gas, liquid, and solid fuel fires. The three‐zone smoke model with three layers is determined by the vertical thermal stratification, and their physical thermal properties are computed. The application of modified method on typical gas fuel, liquid fuel, and simple solid‐fuel fires is verified. The prediction accuracy is examined quantitatively by the cosine similarity comparison of predicted results with the experimental data. In addition, the ventilation effects on the predicted results are also explored. Results show that the application of three‐zone model improves the HRR prediction accuracy, because it can accurately capture the mixing behavior from the upper layer to the lower layer. The effect of ventilation on modified CDG method is positive as the ventilation enhances the smoke mixing and the smoke distribution in each layer is relatively uniform.
Effects of chlorogenic acid, epicatechin gallate, and quercetin on mucin expression and secretion in the Caco‐2/HT29‐MTX cell model
Authors: Volstatova Tereza , Marchica Alessandra , Hroncova Zuzana , Bernardi Rodolfo , Doskocil Ivo , Havlik Jaroslav 
Published: February 2019

AbstractMucins are a family of large glycoproteins that represent the major structural components of the mucus and are encoded by 20 different mucin genes. Mucin expression can be modulated by different stimuli. In this study, we analyzed four mucins (MUC2, MUC3, MUC13, and MUC17) in coculture of Caco‐2/HT29‐MTX cells to demonstrate the variation in gene expression in the presence of antioxidant compounds like chlorogenic acid, epicatechin gallate, and quercetin (apple, tea, and coffee polyphenols, respectively). coculture of Caco‐2/HT29‐MTX cells was treated with polyphenols, and the expression of four mucins was determined by reverse‐transcriptase PCR. In addition, the secretion levels of MUC2 were established by enzyme‐linked immunoassay (ELISA) analysis. The results showed that each polyphenol compound induces different expression patterns of the mucin genes. Statistically significant up‐regulation of MUC17 was observed following incubation with epicatechin gallate and quercetin. ELISA results did not prove any significant differences in protein levels of MUC2 after treatment by the polyphenol compounds. The polyphenols considered in this study may influence mucin secretion and act on diverse salivary substrates to change the barrier properties of mucins for mucus secretion in different ways.
Importance of incorporating intraoperative neuromonitoring of the external branch of the superior laryngeal nerve in thyroidectomy: A review and meta‐analysis study
Authors: Naytah Mai , Ibrahim Iman , da Silva Sabrina 
Published: February 2019

AbstractBackgroundVoice changes are frequently reported after thyroidectomy due to injury to the external branch of the superior laryngeal nerve (EBSLN) and paralysis of the cricothyroid muscle, The objective is to evaluate the advantage of intraoperative neuromonitoring (IONM) in identifying EBSLN during thyroid surgery.MethodsData sources were MEDLINE, PubMed, Web of Science, and Cochrane Library from January 1, 1995, through July 1, 2018. Published studies of adult patients who had thyroid surgery and an attempt to identify EBSLN done by conventional methods and/or IONM were selected.ResultsSeven studies met all inclusion criteria. Patients who had IONM during thyroid surgery had a significantly increased number of identified EBSLN at risk, compared to the control group.ConclusionThe use of IONM during open thyroid surgery increases EBSLN identification/visualization, and hence it may decrease the incidence of post‐thyroidectomy voice disorders.
Ten‐year outcome of curative “exclusive” chemotherapy in N0M0 squamous cell carcinoma of the larynx and pharynx with complete clinical response
Authors: Laccourreye Ollivier , Marret Grégoire , Rubin François , Fabre Elizabeth , Badoual Cécile , Oudard Stéphane , Bonfils Pierre , Lisan Quentin 
Published: February 2019

AbstractBackgroundThe aim of our study was to document 10‐year outcome after curative “exclusive” chemotherapy in N0M0 squamous cell carcinoma of the larynx and pharynx.MethodsRetrospective nonrandomized analysis of an inception cohort of 191 patients. Platinum salt and 5‐fluorouracil were used in all patients.ResultsThe 3‐, 5‐, 10‐year overall actuarial survival and local control estimates were 83.3%, 74.4%, and 55.4% and 62.1%, 62.1%, and 55.3%, respectively. Main causes of death were metachronous second primary cancer (n = 39) and intercurrent disease (n = 28). No clinical variables were associated with increased risk of local recurrence. Salvage treatment resulted in 94.7% ultimate local control and 88.4% organ preservation. “Exclusive” chemotherapy was considered “beneficial” in 62.3% and “detrimental” in 7.8% of cases.ConclusionThe high rate of local recurrence may be thought not to justify this treatment. Nevertheless, many patients avoided surgery and remained free of disease. Therefore, this approach deserves further study in the era of immune checkpoints inhibitors.
Measuring the effect of health on the income of people living in extreme poverty: A comparative cross‐sectional analysis
Authors: Liu Xuejiao , Zhang Liang , He Ruibo , Li Zhong , Wu Yue , Li Boyang 
Published: February 2019

SummaryBackgroundThrough a review of the literature, we have observed that existing studies primarily focus on defining and measuring poverty, identifying the factors that affect poverty, and proposing anti‐poverty strategies. The impact of health on income in the context of extreme poverty has not been adequately studied. In China, 30 million people live below the poverty line, and poverty caused by illness accounts for nearly 44% of the total number of recorded incidents. Health impaired by disease has become the largest obstacle to escaping extreme poverty.ObjectiveTo determine whether health has a greater effect on the incomes of individuals in the extreme poverty group compared with the nonimpoverished group.MethodsThe poverty threshold of China in 2010 was adopted for the definition of extreme poverty. The China Health and Nutrition Survey (CHNS) Database 2014 was selected as the data source. Ordinary least squares (OLS) test was conducted to estimate the model, and the endogeneity of the variables was analyzed by the random effects model. Waist‐to‐hip ratio (WHR) was used instead of body mass index (BMI) to perform the robustness test.ResultsWe found that the influence of individual health conditions on income was augmented in the case of extreme poverty, which indicates that health indeed influences income more strongly for individuals in the extreme poverty group.ConclusionsIn addition to education, investment, and social security projects, further public policy attention should be given to the improvement of the health status of the extremely impoverished population.
Success rates of early versus late initiation of external cephalic version
Authors: Lavie Anat , Reicher Lee , Avraham Sarit , Ram Maya , Maslovitz Sharon 
Published: February 2019

AbstractObjectiveTo determine optimum timing of external cephalic version (ECV).MethodsA retrospective cohort study was conducted at a tertiary hospital in Israel between February 1, 2016, and July 1, 2017. Healthy primiparous women with breech presentation were offered either early ECV (35–36 weeks; n=54) or late ECV (37–38 weeks; n=106). Group assignment was according to the patient's preference and physician availability. The primary outcome was the rate of cephalic presentation at delivery. Secondary outcomes included rate of cesarean delivery, presentation of fetus after the first and last ECVs, and serious fetal complications.ResultsThe incidence of undergoing more than two ECV attempts was 18.5% in the early ECV group and 5.6% in the late ECV group (P=0.039). The incidence of cephalic presentation after the first ECV was 72.2% in the early ECV group versus 66.0% in the late ECV group (P=0.048). By contrast, no statistically significant between‐group differences were found for presentation at delivery or rate of cesarean delivery. The other outcomes were also similar.ConclusionEarly initiation of ECV among primiparous women increased the chance of immediate cephalic presentation; however, it had no effect on presentation at delivery or cesarean delivery rate.
Increased risk of maternal complications from repeat pregnancy among adolescent women
Authors: Maravilla Joemer C. , Betts Kim S. , Alati Rosa 
Published: February 2019

AbstractObjectiveTo evaluate the risk of adverse maternal outcomes among adolescents experiencing a repeat pregnancy in the Philippines.MethodsData were analyzed from four waves (1998–2013) of a cross‐sectional nationally representative survey. We included 2518 non‐nulliparous and non‐pregnant women aged 15–44 years with an interpregnancy interval (IPI) of 24 months or less. Multivariate logistic regression was used to measure the association of repeat pregnancy with adverse maternal outcomes by age group (11–19, 20–24, and 25–45 years), accounting for clustering within each respondent. A stratified analysis by IPI (≤24 vs >24 months) was conducted among 11–19 year olds.ResultsNo association was observed between repeat pregnancy and low birthweight among adolescent mothers. A second pregnancy increased the risk of pregnancy (adjusted odds ratio [OR] 10.49, 95% confidence interval [CI] 4.00–27.49) and labor (adjusted OR 3.61, 95% CI 1.61–8.09) complications among adolescent women (11–19 years). Interaction tests showed there was a significant increase in these risks as compared with older women. Stratified analysis by IPI did not modify the observed effect for either outcome.ConclusionRepeat pregnancy among Filipino adolescents increased the risk of pregnancy and labor complications irrespective of IPI.
Efficient intrahepatic tumor generation by cell sheet transplantation to fabricate orthotopic hepatocarcinoma‐bearing model mice for drug testing
Authors: Akimoto Jun , Nakayama Masamichi , Takagi Soichi , Okano Teruo 
Published: February 2019

AbstractSubcutaneous tumor‐bearing mice are commonly used to evaluate antitumor activity in preclinical studies of anticancer drugs. However, these models often exhibit excessive antitumor responses to anticancer drug candidates. In this study, intrahepatic tumor‐bearing mice as orthotopic tumor models were fabricated by transplanting hepatocarcinoma cell monolayers (sheets) to investigate differences in ectopic versus orthotopic antitumor response. Cell sheets, harvested from temperature‐responsive cell culture dishes using thin gelatin gel supporters, were transferred onto mouse liver surfaces. Cell sheet transplantation drastically improved intrahepatic tumor formation compared with direct intrahepatic injection of dispersed cells. In particular, all cell sheet‐transplanted mice formed well‐developed tumors inside the liver following removal of the mesothelial membrane at the liver surface. Notably, these mice exhibited comparable life spans, indicating similar intrahepatic tumor development rates. Antitumor activity of doxorubicin (DOX) was examined using both subcutaneous and intrahepatic tumor‐bearing mice. Although DOX administration yielded decreased subcutaneous tumor volumes, intrahepatic tumors exhibited no significant antitumor response. The results were considered to represent pharmacokinetic and histological structure differences between ectopic and orthotopic tumors, and partially supported the clinical uses of DOX. Therefore, cancer cell sheet transplantation constitutes a promising method to fabricate intrahepatic tumor‐bearing mice for drug screening test in preclinical studies. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1071–1079, 2019.
Immune therapy of melanoma: Overview of therapeutic vaccines
Authors: Payandeh Zahra , Yarahmadi Maral , Nariman‐Saleh‐Fam Ziba , Tarhriz Vahideh , Islami Maryam , Aghdam Abdolreza Mehdinavaz , Eyvazi Shirin 
Published: February 2019

AbstractMelanoma is the most serious type of skin cancer which develops from the occurrence of genetic mutations in the melanocytes. Based on the features of melanoma tumors such as location, genetic profile and stage, there are several therapeutic strategies including surgery, chemotherapy, and radiotherapy. However, because of the appearance resistance mechanisms, the efficiency of these treatments strategies may be reduced. It has been demonstrated that therapeutic monoclonal antibodies can improve the efficiency of melanoma therapies. Recently, several mAbs, such as nivolumab, pembrolizumab, and ipilimumab, were approved for the immunotherapy of melanoma. The antibodies inhibit immune checkpoint receptors such as CTL4 and pd‐1. Another therapeutic strategy for the treatment of melanoma is cancer vaccines, which improve clinical outcomes in patients. The combination therapy using antibodies and gene vaccine give us a new perspective in the treatment of melanoma patients. Herein, we present the recent progressions in the melanoma immunotherapy, especially dendritic cells mRNA vaccines by reviewing recent literature.
Effect of Silica Nanoparticles on Wormlike Micelles with Different Entanglement Degrees
Authors: Zhao Mingwei , Gao Zhibin , Dai Caili , Sun Xin , Zhang Yue , Yang Xiujie , Wu Yining 
Published: February 2019

AbstractEnhancing the viscoelastic properties of wormlike micelles by adding nanoparticles has been widely reported. Various mechanisms have been proposed to explain how nanoparticles strengthen the network formed by wormlike micelles. It remains unclear whether nanoparticles produce the same effect on systems with different entanglement degrees. To clarify this issue, the concentration of potassium chloride was used to control the entanglement degree of wormlike micelles. The rheological behavior of different nanoparticle‐enhanced wormlike micellar systems (NEWMS) was investigated using rheology. Three critical parameters including zero‐shear viscosity (η0), relaxation time (τR), and contour length (L) were calculated to analyze the effect of nanoparticles on the different wormlike micellar systems. An appropriate mechanism describing the interaction between nanoparticles and wormlike micelles with different structures was proposed.
Identification of volatile compounds and odour activity values in quinoa porridge by gas chromatography–mass spectrometry
Authors: Zhang Yiru , Zhang Shuwei , Fan Weixin , Duan Ming , Han Yuanhuai , Li Hongying 
Published: February 2019

AbstractBACKGROUNDQuinoa porridge is becoming popular among Asian for its nutritional values; hence, it is important to understand its aroma characteristics.RESULTSVolatile compounds in porridge of 30 quinoa varieties were determined by gas chromatography‐mass spectrometry combined with headspace‐solid phase micro‐extraction. In total, 53 volatile compounds were detected and grouped into 14 alkanals, four alcohols, seven ketones, 10 alkanes, 10 acids and esters, and eight heterocycles. The relative content of alkanes (22.97%), acids and esters (44.33%) was comparatively high, although alkanals (11.75%) may dominate the aroma. Most of the compounds were similar with respect to types and numbers, although they varied in amount, whereas 11 compounds varied significantly among different varieties. The 30 varieties could be divided into eight groups based on the concentrations of volatile compounds, although the same varieties would be divided into four groups if based on the relative odour activity values of twelve variable aroma compounds.CONCLUSIONNine compounds were identified as the main contributors to the quinoa porridge aroma, including hexanal, 1‐octen‐3‐ol, 2‐pentylfuran, nonanal, (E,E)‐2,4‐decadienal and 6,10‐dimethyl‐5,9‐undecadien‐2‐one. Heptanal, benzeneacetaldehyde and decanal may play roles in harmonizing the overall aroma. It is also interesting to note that 6,10,14‐trimethyl‐2‐pentadecanone, with a slightly fatty aroma, showed a high content in all varieties. © 2019 Society of Chemical Industry
Super‐Resolution Ultrasound Imaging of Skeletal Muscle Microvascular Dysfunction in an Animal Model of Type 2 Diabetes
Authors: Ghosh Debabrata , Peng Jun , Brown Katherine , Sirsi Shashank , Mineo Chieko , Shaul Philip W. , Hoyt Kenneth 
Published: February 2019

ObjectivesTo evaluate the use of super‐resolution ultrasound (SR‐US) imaging for quantifying microvascular changes in skeletal muscle using a mouse model of type 2 diabetes.MethodsStudy groups were young, standard chow–fed male C57BL/6J mice (lean group) and high fat diet–fed older mice (obese group). After an overnight fast, dynamic contrast‐enhanced US imaging was performed on the proximal hind limb adductor muscle group for 10 minutes at baseline and again at 1 and 2 hours during administration of a hyperinsulinemic‐euglycemic clamp. Dynamic contrast‐enhanced US images were collected on a clinical US scanner (Acuson Sequoia 512; Siemens Healthcare, Mountain View, CA) equipped with a 15L8 linear array transducer. Dynamic contrast‐enhanced US images were processed with a spatiotemporal filter to remove tissue clutter. Individual microbubbles were localized and counted to create an SR‐US image. A frame‐by‐frame analysis of the microbubble count was generated (ie, time‐microbubble count curve [TMC]) to estimate tissue perfusion and microvascular blood flow. The conventional time‐intensity curve (TIC) was also generated for comparison.ResultsIn vivo SR‐US imaging could delineate microvascular structures in the mouse hind limb. Compared with lean animals, insulin‐induced microvascular recruitment was attenuated in the obese group. The SR‐US‐based TMC analysis revealed differences between lean and obese animal data for select microvascular parameters (P < .04), which was not true for TIC‐based measurements. Whereas the TMC and TIC microvascular parameters yielded similar temporal trends, there was less variance associated with the TMC‐derived values.ConclusionsSuper‐resolution US imaging is a new modality for measuring the microvascular properties of skeletal muscle and dysfunction from type 2 diabetes.