bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of 17 mRNAs and a miRNA as an integrated prognostic signature for lung squamous cell carcinoma
Authors: Zhang Jingyun , Bing Zhitong , Yan Peijing , Tian Jinhui , Shi Xiue , Wang Yongfeng , Yang Kehu 
Published: June 2019

AbstractBackgroundGene signatures for predicting the outcome of lung squamous cell carcinoma (LUSC) have been employed for many years. However, various signatures have been applied in clinical practice. Therefore, in the present study, we aimed to filter out an effective LUSC prognostic gene signature by simultaneously integrating mRNA and microRNA (miRNA).MethodsFirst, based on data from the Cancer Genome Atlas (TCGA) (https://www.cancer.gov/tcga), mRNAs and miRNAs that were related to overall survival of LUSC were obtained by the least absolute shrinkage and selection operator method. Subsequently, the predicting effect was tested by time‐dependent receiver operating characteristic curve analysis and Kaplan–Meier survival analysis. Next, related clinical indices were added to evaluate the efficiency of the selected gene signatures. Finally, validation and comparison using three independent gene signatures were performed using data from the Gene Expression Omnibus database (https://www.ncbi.nlm.nih.gov/geo).ResultsOur data showed that the prognostic index (PI) contained 17 mRNAs and one miRNA. According to the best normalized cut‐off of PI (0.0247), the hazard ratio of the PI was 3.40 (95% confidence interval = 2.33–4.96). Moreover, when clinical factors were introduced, the PI was still the most significant index. In addition, only two Gene Ontology terms withp < 0.05 were reported. Furthermore, validation implied that, using our 18‐gene signature, only hazard ratio = 1.36 (95% confidence interval = 1.01–1.83) was significant compared to the other three groups of gene biomarkers.ConclusionsThe 18‐gene signature selected based on data from the TCGA database had an effective prognostic value for LUSC patients.
New parameters of ultrafast dynamic contrast‐enhanced breast MRI using compressed sensing
Authors: Honda Maya , Kataoka Masako , Onishi Natsuko , Iima Mami , Ohashi Akane , Kanao Shotaro , Nickel Marcel Dominik , Toi Masakazu , Togashi Kaori 
Published: June 2019

BackgroundUltrafast dynamic contrast‐enhanced (UF‐DCE) breast MRI is considered a promising method of accelerated breast MRI. However, the value of new kinetic parameters derived from UF‐DCE need clinical evaluation.PurposeTo evaluate the diagnostic performance of the maximum slope (MS), time to enhancement (TTE), and time interval between arterial and venous visualization (AVI) derived from UF‐DCE MRI using compressed sensing (CS).Study TypeRetrospective.PopulationSeventy‐five patients with histologically proven breast lesions. The total number of analyzed lesions was 90 (61 malignant and 29 benign).Field Strength/Sequence3T MRI with UF‐DCE MRI based on the 3D gradient‐echo volumetric interpolated breath‐hold examination (VIBE) sequence using incoherent k‐space sampling combined with a CS reconstruction followed by conventional DCE MRI.AssessmentThe diagnostic performance of the MS, TTE, AVI, and conventional kinetic analysis was analyzed and compared with histology.Statistical TestsWilcoxon rank sum test, receiver operating characteristic analysis.ResultsThe MS was larger and the TTE and AVI were smaller for malignant lesions compared with benign lesions: MS: 29.3%/s and 18.4%/s (P < 0.001), TTE: 7.0 and 12.0 seconds (P < 0.001), AVI: 2.7 and 4.4 frames (P = 0.006) for malignant and benign lesions. The discriminating power of the MS (area under the curve [AUC], 0.76) was slightly better than that of conventional kinetic analysis (AUC, 0.69) and comparable to that of the TTE and AVI (AUC, 0.78 and 0.76 for TTE and AVI, respectively). Invasive lobular carcinoma had smaller MS (21.8%/s) among malignant lesions (29.3%/s).Data ConclusionThe MS, TTE, and AVI can be used to evaluate breast lesions with clinical performance equivalent to that of conventional kinetic analysis. These parameters vary among histologies.Level of Evidence: 3Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2020;51:164–174.
High‐performance liquid chromatographic and subcritical fluid chromatographic separation of α‐arylated ß‐carboline, N‐alkylated tetrahydroisoquinolines and their bioisosteres on polysaccharide‐based chiral stationary phases
Authors: Bajtai Attila , Lajkó Gyula , Németi Gábor , Szatmári István , Fülöp Ferenc , Péter Antal , Ilisz István 
Published: June 2019

AbstractNew, pharmacologically interesting chiral amino compounds, namely, stereoisomers of α‐hydroxynaphthyl‐ß‐carboline, benz[d]azepine and benz[c]azepine analogs as well as N‐α‐hydroxynaphthylbenzyl‐substituted isoquinolines were enantioseparated by high‐performance liquid chromatographic and subcritical fluid chromatographic methods on polysaccharide‐based chiral stationary phases. Separation of the stereoisomers was optimized in both subcritical fluid chromatography and normal phase liquid chromatographic modes by investigating the effects of the composition of the bulk solvent, temperature, and the structures of the analytes and selectors. Both normal phase liquid chromatography and subcritical fluid chromatography exhibited satisfactory performance, albeit with somewhat different effectiveness in the separation of the stereoisomers studied. The optimized methods offer the possibility to apply preparative‐scale separations thereby enabling further pharmacological investigations of the enantiomers.
The role of integrins in the pathogenesis of inflammatory bowel disease: Approved and investigational anti‐integrin therapies
Authors: Dotan Iris , Allez Matthieu , Danese Silvio , Keir Mary , Tole Swati , McBride Jacqueline 
Published: June 2019

AbstractInflammatory bowel disease (IBD) is characterized by uncontrolled inflammation in the gastrointestinal tract. The underlying pathobiology of IBD includes an increase in infiltrating gut‐homing lymphocytes. Although lymphocyte homing is typically a tightly regulated and stepwise process involving multiple integrins and adhesion molecules expressed on endothelial cells, the distinct roles of integrin‐expressing immune cells is not fully understood in the pathology of IBD. In this review, we detail the involvement of integrins expressed on specific lymphocyte subsets in the pathogenesis of IBD and discuss the current status of approved and investigational integrin‐targeted therapies.
Tea Consumption and Health Outcomes: Umbrella Review of Meta‐Analyses of Observational Studies in Humans
Authors: Yi Mengshi , Wu Xiaoting , Zhuang Wen , Xia Lin , Chen Yi , Zhao Rui , Wan Qianyi , Du Liang , Zhou Yong 
Published: June 2019

ScopeThe aim of this article is to conduct an umbrella review to study the strength and validity of associations between tea consumption and diverse health outcomes.Methods and resultsMeta‐analyses of observational studies examining associations between tea consumption and health outcomes in all human populations and settings are screened. The umbrella review identifies 96 meta‐analyses with 40 unique health outcomes. Tea consumption shows greater benefits than harm to health in this review. Dose–response analyses of tea consumption indicates reduced risks of total mortality, cardiac death, coronary artery disease, stroke, and type 2 diabetes mellitus with increment of two to three cups per day. Beneficial associations are also found for several cancers, skeletal, cognitive, and maternal outcomes. Harmful associations are found for esophageal and gastric cancer when the temperature of intake is more than 55–60 °C.ConclusionTea consumption, except for very hot tea, seems generally safe at usual levels of intake, with summary estimates indicating the largest reduction for diverse health outcomes at two to three cups per day. Generally, tea consumption seems more beneficial than harmful in this umbrella review. Randomized controlled trials are further needed to understand whether the observed associations are causal.
Birth of a marmoset following injection of elongated spermatid from a prepubertal male
Authors: Ogonuki Narumi , Abe Yukiko , Kurotaki Yoko K. , Nakao Kazuki , Aiba Atsu , Sasaki Erika , Ogura Atsuo 
Published: June 2019

AbstractThe common marmoset is a small nonhuman primate in which the application of transgenesis and genetic knockout techniques allows the generation of gene‐modified models of human diseases. However, its longer generation time than that of rodents is a major obstacle to the widespread use of gene‐modified marmosets for biomedical research. In this study, we examined the feasibility of shortening the generation time by using prepubertal marmoset males as gamete donors. We collected late round stage spermatids (Steps 5–7), elongated spermatids, and testicular spermatozoa from the testis of a prepubertal 11‐month‐old male marmoset and injected them into in vitro‐matured oocytes. After 7 days in culture, two embryos from elongated spermatid injection and two embryos from sperm injection were transferred into two separate recipient females. The recipient female that received elongated spermatid injection‐derived embryos became pregnant and gave birth to one female infant. This is the first demonstration that a spermatid from a prepubertal male primate can support full‐term development. Using this method, we can expect to obtain offspring of gene‐modified males 6 months to a year earlier than with natural mating
The validation of the Dutch OAB‐q SF: An overactive bladder symptom bother and health‐related quality of life short‐form questionnaire
Authors: Groenendijk Ilse M. , Scheepe Jeroen R. , Noordhoff Toscane C. , Blok Bertil F. M. 
Published: June 2019

AbstractAimsThe overactive bladder quality of life short‐form questionnaire (OAB‐q SF) evaluates both symptom bother and health‐related quality of life in patients with OAB, a highly prevalent disease. The objective of this study was to translate and validate a Dutch version of the OAB‐q SF.MethodsThe translation into Dutch and validation process of the OAB‐q SF was performed according to standardized guidelines. Patients with OAB who visited the department of Urology outpatient clinic completed the questionnaires OAB‐q SF, European Quality of life 5‐Dimension 5‐Level questionnaire (EQ‐5D‐5L), Urogenital Distress Inventory 6 (UDI‐6), and the International Consultation on Incontinence Questionnaire Overactive Bladder (ICIQ‐OAB) at baseline (test) and 2 weeks later (retest). A reference group from the department of Allergology outpatient clinic completed the same questionnaires once. The evaluated measurement properties included content validity, internal consistency, reproducibility, criterion validity, and construct validity.ResultsFifty‐two patients were included in the study group and 51 references were included. The content validity was adequate and the internal consistency was excellent (Cronbach's α > 0.80). The reproducibility was good with intraclass correlation coefficients higher than 0.70. Patient's OAB‐q SF scores were moderately to strongly correlated with the UDI‐6, ICIQ‐OAB, and the EQ‐5D‐5L confirming the criterion validity. A good construct validity was demonstrated with significant higher scores of the OAB‐q SF score in patients compared to references.ConclusionsThe Dutch OAB‐q SF is a reliable and valid measure to evaluate symptom bother and health‐related quality of life in patients with OAB.
A novel minimum weight formulation of topology optimization implemented with reanalysis approach
Authors: Long Kai , Gu Chunlu , Wang Xuan , Liu Jie , Du Yixian , Chen Zhuo , Saeed Nouman 
Published: June 2019

SummaryIn this paper, we develop an efficient diagonal quadratic optimization formulation for minimum weight design problem subject to multiple constraints. A high‐efficiency computational approach of topology optimization is implemented within the framework of approximate reanalysis. The key point of the formulation is the introduction of the reciprocal‐type variables. The topology optimization seeking for minimum weight can be transformed as a sequence of quadratic program with separable and strictly positive definite Hessian matrix, thus can be solved by a sequential quadratic programming approach. A modified sensitivity filtering scheme is suggested to remove undesirable checkerboard patterns and mesh dependence. Several typical examples are provided to validate the presented approach. It is observed that the optimized structure can achieve lighter weight than those from the established method by the demonstrative numerical test. Considerable computational savings can be achieved without loss of accuracy of the final design for 3D structure. Moreover, the effects of multiple constraints and upper bound of the allowable compliance upon the optimized designs are investigated by numerical examples.
Fish exploitation in medieval and early modern Switzerland: Evidence from the ichthyoarchaeological record and historical sources
Authors: Häberle Simone , Hüster Plogmann Heide 
Published: June 2019

AbstractIn Switzerland, fish played a substantial role in people's diets both in medieval times and in the early modern period. In this paper, we present an overview of ichthyoarchaeological data from 20 years of research, mainly from the northern and eastern regions of Switzerland. The integration of results of faunal analyses from 20 archaeological sites, and the study of historical sources provided information on different modes of fish exploitation during these periods. This study shows that the fish remains from archaeological sites correspond with the descriptions in the written records: Both sources point to local fisheries within Swiss freshwater systems. These fisheries were strongly regulated and often selective. Large freshwater fish of high quality was mainly caught by professional fishermen and eaten by the aristocratic and clerical classes, whereas small or young specimens were caught by all social classes even though their catch was strongly restricted. Furthermore, both sources provide evidence for an inland freshwater fish trade of selected species and for the import of herring and suggest the practice of different freshwater fish cultivation methods.
Ontogenetic dental patterns in Pleistocene hyenas (Crocuta crocuta Erxleben, 1777) and their palaeobiological implications
Authors: Jimenez Israel Jesus , Sanz Montserrat , Daura Joan , De Gaspar Ignacio , García Nuria 
Published: June 2019

AbstractDuring the Pleistocene, humans and hyenas co‐occurred at sites, which included cavities and rock‐shelters, accumulating bone assemblages attributable to both the hominids and carnivores. Studies of these co‐occurrences have given rise to much debate about the relationships established, suggesting that an interpretation of the nature of the biological activities conducted could be useful for understanding predator behaviour and for reconstructing the palaeobiology of these sites. Dentition analysis is an effective technique, employed in zooarchaeological studies, to interpret the use of shared spaces. However, to date, tooth development studies aimed at determining the age of an archaeological assemblage are scarce. At Pleistocene sites, isolated hyena teeth are typically the most common elements. In Terrasses de la Riera dels Canyars (Gavà, Barcelona, NE Iberian Peninsula), a fluvial deposit dated at ̴39.6 ka cal Before the Present (BP) (Heinrich Stadial 4) with a sizeable record of large mammals and just a few lithic tools (Aurignacian), cranial and postcranial hyena bones are remarkably well‐preserved, exhibiting all their ontogenetic stages. Here, we conduct an analysis of dental ontogeny (employing X‐ray imaging techniques), wear and replacement, and propose four age categories for hyena juveniles and one category for subadults based on complete (or almost complete) mandibles and maxillae. By employing these five, more detailed, age clusters, the minimum number of individuals is found to increase. Application of the method to the site's isolated teeth confirms its validity. The hyena mortality pattern recorded at Canyars, together with descriptions of extant hyena behaviour, indicate that the site was used primarily as a communal den.
Can Light‐Touch College‐Going Interventions Make a Difference? Evidence from a Statewide Experiment in Michigan
Authors: Hyman Joshua 
Published: June 2019

AbstractI conduct a statewide experiment in Michigan with nearly 50,000 high‐achieving high school seniors. Treated students are mailed a letter encouraging them to consider college and providing them with the web address of a college information website. I find that very high‐achieving, low‐income students, and very high‐achieving, minority students are the most likely to navigate to the website. Small changes to letter content affect take‐up. For example, highlighting college affordability induces 18 percent more students to the website than highlighting college choice, and 37 percent more than highlighting how to apply to college. I find a statistically precise zero impact on college enrollment among all students who were mailed the letter. However, low‐income students experience a small increase in the probability that they enroll in college, driven by increases at four‐year institutions. An examination of persistence through college, while imprecise, suggests that the students induced into college by the intervention persist at a lower rate than the inframarginal student.
Gamma‐secretase complex‐dependent intramembrane proteolysis of CD147 regulates the Notch1 signaling pathway in hepatocellular carcinoma
Authors: Yong Yu‐Le , Zhang Ren‐Yu , Liu Ze‐Kun , Wei Ding , Shang Yu‐Kui , Wu Jiao , Zhang Zhi‐Yun , Li Can , Chen Zhi‐Nan , Bian Huijie 
Published: June 2019

AbstractThe γ‐secretase complex is a presenilin‐dependent aspartyl protease involved in the intramembranous cleavage of various type I transmembrane proteins. As a type I transmembrane protein, CD147 is highly expressed in hepatoma cells and promotes cell proliferation, migration, and invasion. However, the direct underlying mechanism of how CD147 promotes cancer cell proliferation is unknown. Here, we demonstrated that CD147 undergoes an intramembranous cleavage by the γ‐secretase at lysine 231 to release its intracellular domains (ICDs). The nuclear translocation of the CD147ICD regulated Notch1 expression by directly binding to theNOTCH1promoter and promoted the activation of the Notch signaling pathway. Simultaneously, overexpression of CD147ICD promoted cancer cell proliferation via Notch1 signaling. In 102 cases of human hepatocellular carcinoma (HCC) tissues, patients with a high positive rate of nuclear CD147ICD expression had a significantly poor overall survival compared with patients with a low positive rate of nuclear CD147ICD expression. We confirmed that nuclear CD147ICD predicted a poor prognosis in human HCC. The combined therapy of the γ‐secretase complex inhibitor and CD147‐directed antibody showed better efficacy than monotherapy in orthotopic transplantation HCC mouse models. In conclusion, CD147 is cleaved by the γ‐secretase and releases CD147ICD to the cell nucleus, promoting Notch1 expression via direct binding to theNOTCH1promoter. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Phthalic anhydride promoted ring‐opening polymerization of cyclohexene oxide catalyzed by dinuclear chromium complex supported by piperazine‐bridged [ONSO] ligand
Authors: Wang Meige , Huang Jie , Xu Yunpeng , Han Bing , Duan Zhongyu 
Published: June 2019

AbstractNew bimetallic chromium complexes bearing a piperazine‐bridged tetradentate‐dianionic iminethiobis(phenolate) (ONSO) ligand and an ancillary chloride group (complex 1) have been developed. Of interest, it was found that the complex 1 catalyzed copolymerization of cyclohexene oxide with phthalic anhydride (PA) gives a product with more than 97% ether linkage content, i.e. selective synthesis of poly(cyclohexene oxide), which is in sharp contrast to the mononuclear [ONSO]CrCl analogue where a resulting polymer with more than 86% ester units is achieved under identical conditions. The influences of PA loading, reaction time and temperature on the polymerization were investigated. According to the kinetics data, the apparent activation energy of the ring‐opening polymerization of cyclohexene oxide is 12.5 kJ mol−1. A cationic polymerization mechanism is proposed according to the polymer structure and the interaction between metal complex 1 and PA. © 2019 Society of Chemical Industry
Protein Corona Modulates Distribution and Toxicological Effects of Silver Nanoparticles In Vivo
Authors: Barbir Rinea , Goessler Walter , Ćurlin Marija , Micek Vedran , Milić Mirta , Vuković Barbara , Milić Marija , Ljubojević Marija , Domazet Jurašin Darija , Vinković Vrček Ivana 
Published: June 2019

AbstractExploitation of silver nanoparticles (AgNPs) in biomedicine represents more than one third of their overall applications. Despite their wide use, detailed toxicokinetic data and information on their action mechanisms in vivo are still scarce. One important obstacle is their fate and transformation patterns in biological environments where AgNPs get a “new face” after interaction with biomolecules, particularly proteins. The impact of protein corona on AgNP effects in vivo is eludicated. The in vivo effects of AgNPs prepared with two different protein coronas, albumin, and metallothionein, with polymer‐coated AgNPs are compared in male and female Wistar rats after intravenous administration. The results demonstrate that the character of the protein coronas on the AgNP surface affects not only distribution, but also oxidative stress response and genotoxicity in tissues of rat exposed to AgNPs. Additionally, sex‐related effects are observed. By emphasizing the importance of protein corona formation and sex‐related response, the obtained data support a reliable evidence base needed for assessing the health risks of the steadily increasing human exposure to AgNPs.
Design considerations for photovoltaic panel arrays made from aluminium: a case study
Authors: Bardopoulos Foivos , Papagiannopoulos George , Pnevmatikos Nikos 
Published: June 2019

AbstractThis paper presents the design considerations for typical photovoltaic panel arrays having aluminium members. Section and member design checks are performed according to Eurocode 9 on the basis of the wind, snow and seismic loads of Eurocodes 1 and 8. Improvements to the design are then sought, starting by reducing the distance between the vertical posts and then by changing the thickness of specific sections. Following that, the effects of member imperfections and connection flexibility are studied using a reduced flexural rigidity and different rotational stiffness values respectively. The degree of dynamic coupling when the array is placed on the top floor of an existing building as well as the influence of founding the vertical posts on compliant ground are also evaluated.