bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tid1‐S attenuates LPS‐induced cardiac hypertrophy and apoptosis through ER‐a mediated modulation of p‐PI3K/p‐Akt signaling cascade
Authors: Chao Chun‐Nun , Lo Jeng‐Fan , Khan Farheen B. , Day Cecilia H. , Lai Chao‐Hung , Chen Chia‐Hua , Chen Ray‐Jade , Viswanadha Vijaya P. , Kuo Chia‐Hua , Huang Chih‐Yang 
Published: May 2019

AbstractMyocardial dysfunction is clinically relevant? repercussion that follows sepsis. Tid 1 protein has been implicated in many biological process. However, the role of Tid 1 in lipopolysaccharide (LPS)‐induced cardiomyocyte hypertrophy and apoptosis remains elusive. In the current research endeavor, we have elucidated the role of Tid1‐S on LPS‐induced cardiac hypertrophy and apoptosis. Interestingly, we found that overexpression of Tid1‐S suppressed TLR‐4, NFATc3, and BNP protein expression which eventually led to inhibition of LPS‐induced cardiac hypertrophy. Moreover, Tid1‐S overexpression attenuated cellular apoptosis and activated survival proteins p‐PI3K and pser473Akt. Besides this, Tid1‐S overexpression enhanced ER‐a protein expression. Collectively, our data suggest that Tid1‐S plausibly enhance ER‐a protein and further activate p‐PI3K and p
ser473Akt survival protein expression; which thereby led to attenuation of LPS‐induced apoptosis in cardiomyoblast cells. Interestingly, our data suggest that Tid1‐S is involved in attenuation of cardiomyoblast cells damages induced by LPS.
Effect of embryo cryopreservation on derivation efficiency, pluripotency, and differentiation capacity of mouse embryonic stem cells
Authors: Assadollahi Vahideh , Hassanzadeh Kambiz , Abdi Mohammad , Alasvand Masoud , Nasseri Sherko , Fathi Fardin 
Published: May 2019

AbstractMouse embryonic stem cells (mESCs) are pluripotent cells that have the capability for self‐renewal. One of the most important factors that affect the efficiency of their isolation is the condition of the mouse embryos. The main objective of this study is to isolate mESCs from C57BL/6 frozen/thawed eight‐cell mouse embryos using serum‐free culture. We generated mESCs from blastocysts that developed from frozen/thawed embryos of C57BL/6 mice by the 3i + LIF medium. Assessments of the isolated mESC lines (MUKF‐1, MUKF‐2, and MUKF‐3) included simple karyotype analysis; polymerase chain reaction of the testis‐determining gene (Sry); determination of alkaline phosphatase (ALP) activity; expressions of pluripotent transcription factors Oct4, Rex1, Sox2, and Nanog by reverse transcription polymerase chain reaction; and immunocytochemistry assessment of OCT4 and SSEA‐I expressions at the protein level. We evaluated the ability of these mESC lines to differentiate into three germ layers by embryoid body (EB) formation. The cell doubling time (DT) of isolated mESCs was determined. The 2‐C57 cell line was served as control. Germline competence of the male mESC line (MUKF‐3) was tested through chimeric mouse production. Three independent mESC lines (MUKF‐1, MUKF‐2, and MUKF‐3) were established from five cryopreserved embryos. The MUKF‐1 and MUKF‐2 lines were female, whereas MUKF‐3 was a male mESC line. Karyotype analysis showed that MUKF‐3 had a diploid karyotype, whereas MUKF‐1 and MUKF‐2 had abnormal karyotypes. All three lines had ALP activity and expressed Oct4, Rex1, and Nanog. Immunocytochemistry assessment for OCT4 and SSEA‐I was positive for all three lines. The DT differed in the three mESC lines. MUKF‐1 and MUKF‐3 could form EB and express developmental genes after spontaneous differentiation. These data demonstrated that probably cryopreservation affected the efficiency of derivation, karyotype, DT, expression of pluripotency, developmental genes, and differentiation capacity of the independent mESC lines.
Integrative analyses of triple negative dysregulated transcripts compared with non‐triple negative tumors and their functional and molecular interactions
Authors: Darbeheshti Farzaneh , Rezaei Nima , Amoli Mahsa M. , Mansoori Yaser , Tavakkoly Bazzaz Javad 
Published: May 2019

AbstractTriple‐negative (TN) tumors are a subtype of breast cancer with aggressive behaviors and limited targeted therapies. Microarray studies were not concerned with interactions and functional relations of dysregulated transcripts. Here, we aimed to conduct integrative strategy to analyze gene and miRNA available microarray data as well as bioinformatic analyses to catch a more inclusive picture of pivotal dysregulated transcripts and their interactions in TN tumors. Several online datasets and offline bioinformatic tools were used to detect differentially expressed (DE) transcripts, both protein and nonprotein coding, in TN compared with non‐TN tumors and their functional and molecular interactions.Sixteen upregulated and 58 downregulated genes with a log fold change higher or equal to | 2 | were identified, including nine transcription factors. Coexpression network revealed EN1 as a hub gene, moreover Kaplan–Meier plotter survival analysis indicated that it was an appropriate prognostic marker for TN patients with breast cancer. Functional annotation analysis of protein–protein interaction network showed FOXM1 as an upexpressed and ESR1 as a downexpressed hub genes are suitable targets as far as antitumor protein therapy is concerned in TN breast cancers. The consensus analysis of two microRNA datasets revealed seven DE miRNAs. The gene–transcriptional factor (TF)–miRNA network revealed mir‐135b and mir‐29b are the hub nodes and involved in feedback loops with GATA3. This study suggests that dysregulated TFs and miRNAs have pivotal roles in regulation of TN oncotranscriptomic profile and might become both biomarkers and therapeutic targets.
Association between TL1A gene polymorphisms and systemic lupus erythematosus in a Chinese Han population
Authors: Xu Wang‐Dong , Fu Lu , Liu Xiao‐Yan , Wang Jia‐Min , Yuan Zhi‐Chao , Su Lin‐Chong , Huang An‐Fang 
Published: May 2019

AbstractOur previous studies showed elevated tumor necrosis factor‐like ligand 1 aberrance (TL1A) expression in systemic lupus erythematosus (SLE). However, TL1A polymorphisms with SLE susceptibility remain to be elucidated. In addition, we made meta‐analysis to evaluate the relationship of TL1A polymorphisms and autoimmune diseases owing to inconsistent results. The present research was carried out by 404 SLE, 150 primary Sjogren's syndrome (pSS) patients, and 574 healthy individuals. Three TL1A polymorphisms (rs3810936, rs6478109, rs7848647) were genotyped using TaqMan genotyping assay. Then, the meta‐analysis was performed by collecting the present case‐control study and previously published research. Results showed that genotypes of rs3810936, rs7848647 were different between SLE patients and healthy controls, whereas no significant association was observed in the three polymorphisms and pSS patients. Genotypes distribution of rs6478109, rs7848647 were strongly related to lupus nephritis within SLE (p = 0.004, p = 0.011), respectively. Moreover, combined meta‐analysis consisted of ten comparative research involving 4,305 patients and 5,600 controls. An association between autoimmune diseases and rs6478109 polymorphism was found. Our findings indicate that gene polymorphisms (rs3810936, rs7848647) of TL1A might correlate with lupus.
The spectrum of viral pathogens in children with severe acute lower respiratory tract infection: A 3‐year prospective study in the pediatric intensive care unit
Authors: Li Ya‐Ting , Liang Ying , Ling Ye‐Sheng , Duan Meng‐Qi , Pan Li , Chen Zhuang‐Gui 
Published: May 2019

AbstractBackgroundNo comprehensive analysis is available on the viral etiology and clinical characterization among children with severe acute lower respiratory tract infection (SALRTI) in Southern China.MethodsCohort of 659 hospitalized children (2 months to 14 years) with SALRTI admitted to the Pediatric Intensive Care Unit (PICU) in the Guangzhou from May 2015 to April 2018 was enrolled in this study. Nasopharyngeal aspirate specimens or induced sputum were tested for eight categories respiratory viral targets. The viral distribution and its clinical characters were statistically analyzed.ResultsViral pathogen was detected in 326 (49.5%) of children with SALRTI and there were 36 (5.5%) viral coinfections. Overall, the groups of viruses identified were, in descending order of prevalence: Influenza virus (IFV) (n = 94, 14.3%), respiratory syncytial virus (RSV) (n = 75, 11.4%), human rhinovirus (HRV) (n = 56, 8.5%), adenovirus (ADV) (n = 55, 8.3%), parainfluenza (PIV) (n = 47, 7.1%), human coronavirus (HCoV) (n = 15, 2.3%), human metapneumovirus (HMPV) (n = 14, 2.1%) and human bocavirus (HBoV) (n = 11, 1.7%). The positive rate in younger children (< 5 years) was significantly higher than the positive rate detected in elder children (> 5 years) (52.5% vs 35.1%, P = 0.001). There were clear seasonal peaks for IFV, RSV, HRV, ADV, PIV, and HMPV. And the individuals with different viral infection varied significantly in terms of clinical profiles.ConclusionsViral infections are present in a consistent proportion of patients admitted to the PICU. IFV, RSV, HRV, and ADV accounted for more than two‐thirds of all viral SALRTI. Our findings could help the prediction, prevention and potential therapeutic approaches of SALRTI in children.
The characteristics of moisture recycling and its impact on regional precipitation against the background of climate warming over Northwest China
Authors: Wu Ping , Ding Yihui , Liu Yanju , Li Xiucang 
Published: May 2019

AbstractFrom 1961 to 2015, annual and seasonal precipitation have shown a significant increase over Northwest China (NW). The change of precipitation is a combination result of the changing recycling precipitation (Pw) contributed by local evapotranspiration (ET) and changing precipitation contributed by advection moisture (Po). In this paper, we calculated the ET based on the observation data of meteorological stations and the complementary relationship model. Then used an improved precipitation recycling model to analyse influential factors on the variation of precipitation over the NW from aspects of ET and advection moisture. The annual precipitation recycling ratio (ρ) fluctuates between 4% and 10% and shows an increasing trend at a rate of 0.3% /10a with a significance level of 0.01. ET, which is the internal supplier of the precipitable water, and advection moisture, which is the outside supplier of the precipitable water, both have displayed significantly increasing trends over the NW. The advection moisture contributes precipitation much more than ET, no matter in the annual scale or seasonal scales. In view of the spatial distribution, Pw shows an increasing trend over the whole NW, but distinctly opposite spatial patterns of Po are found between the western area of NW (WNW) and the eastern area of NW (ENW). Such differences caused the precipitation is characterized by a wetting trend in WNW but a drying trend in ENW.
Ovulatory follicular fluid induces sperm phagocytosis by neutrophils, but oviductal fluid around oestrus suppresses its inflammatory effect in the buffalo oviduct in vitro
Authors: Yousef Mohamed S. , Abd‐Elhafeez Hanan H. , Talukder Anup K. , Miyamoto Akio 
Published: May 2019

AbstractWe have recently shown that the conditioned media from bovine oviductal epithelial cell culture suppress sperm phagocytosis by neutrophils, suggesting that the oviduct around oestrus supplies the anti‐inflammatory microenvironment. To investigate the immune response of neutrophils toward the sperm at ovulation in the buffalo oviduct, we examined (a) a detailed distribution of neutrophils in the oviduct in buffaloes, (b) the effect of ovulatory follicular fluid (FF) and oviductal fluid (OF) on sperm phagocytosis by neutrophils, and (c) the interaction of the ovulatory FF with OF on sperm phagocytosis by neutrophils in vitro. Buffalo oviducts were collected from healthy reproductive tracts at a local slaughterhouse. A detailed observation by histological examination and transmission electron microscopy revealed that neutrophils exist in the oviduct epithelium and lumen throughout the oestrous cycle in buffaloes. The number of neutrophils at the oestrus stage was higher in ampulla compared with those in isthmus, whereas they remained relatively constant at the dioestrus stage. Two hours of preincubation of neutrophils with FF enhanced sperm phagocytosis through the formation of neutrophil extracellular traps (NETs) together with H2O2 production, whereas OF around oestrus (eOF) suppressed sperm phagocytosis, NETs formation, and H2O2 production and relieved the above FF‐induced inflammatory response. Our findings show that neutrophils exist in the healthy cyclic oviduct across bovine species, and the OF supplies a strong anti‐inflammatory environment that could minimize the inflammatory effect of the FF that flows into the oviduct lumen after ovulation and supports the occurrence of fertilization.
Specialized naphthoquinones present in Impatiens glandulifera nectaries inhibit the growth of fungal nectar microbes
Authors: Block Anna K. , Yakubova Elena , Widhalm Joshua R. 
Published: May 2019

AbstractThe invasion success of Impatiens glandulifera (Himalayan balsam) in certain parts of Europe and North America has been partially attributed to its ability to compete for bee pollinators with its rich nectar and due to its capacity to produce and release allelopathic 1,4‐naphthoquinones (1,4‐NQs) from its roots and leaves. Given that other 1,4‐NQs present in the digestive fluids of certain carnivorous plants are proposed to control microbial colonization, we investigated the potential for the 1,4‐NQs, 2‐methoxy‐1,4‐naphthoquinone (2‐MNQ) and lawsone, to fulfill an analogous role in the nectaries of I. glandulifera. Both 2‐MNQ and lawsone were detected in the floral nectaries of I. glandulifera at levels comparable to leaves and roots, but were discovered to be at significantly higher levels in its extra‐floral nectaries (EFNs) and to be present in EFN nectar itself. Nectar microbe inhibition assays revealed that the common nectar bacteria Gluconobacter oxydans and Asaia prunellae are not inhibited by 2‐MNQ or lawsone, although both compounds were found to inhibit the growth of the common fungal nectar microbes Metschnikowia reukaufii and Aureobasidium pullulans. Taken together, these findings suggest that 2‐MNQ and lawsone could serve to protect the rich nectar of I. glandulifera against fungal growth. The high abundance of 2‐MNQ and lawsone in I. glandulifera EFNs may also point to an unsuspected mechanism for how allelopathic 1,4‐NQs are leached into the soil where they exhibit their known allelopathic effects.
Genetic basis of surfactant dysfunction in Chinese children: A retrospective study
Authors: Chen Jiehua , Nong Guangmin , Liu Xiuyun , Ji Wei , Zhao Deyu , Ma Hongling , Wang Heping , Zheng Yuejie , Shen Kunling 
Published: May 2019

AbstractObjectiveTo investigate the prevalence of surfactant dysfunction (SD) and the genotype distribution in Chinese childhood interstitial lung disease (chILD).MethodsFrom December 2013 to December 2016, whole exons and splicing regions of surfactant protein (SP)‐B, SP‐C, and adenosine triphosphate (ATP)‐binding cassette subfamily A member 3 (ABCA3) were sequenced in chILD with unknown etiology in five children's medical centers of China. The sequencing was performed by Next‐generation sequencing technique in a molecular genetics laboratory. The clinical and genetic data were reviewed retrospectively.ResultsIn total, 136 patients of age 3 months to 13 years (mean 12.5 ± 9.4 months) were recruited, among which 76 were males. Of the 136 cases of chILD, 13.2% (18 of 136) were diagnosed with SD. In these 18 SD cases, 15 had heterozygous SP‐C deficiencies, two cases had compound heterozygous ABCA3 deficiencies, and no SP‐B deficiency was identified. In SP‐C deficiencies, there were six cases with p.I73T, 2 with p.I73N, 5 with p.V39L, 1 with c.417delA, and 1 case with IVS4, +1G>C. Two cases of ABCA3 mutation were heterozygous with c.1755delC and c.2890G>A; c.3913T>C (R1305W) and exon 13 to 18 deletion. One was negative by sequencing while diagnosed positive by pathology.ConclusionThe proportion of genetic mutation of SD in chILD is 13.2% in China, of which SP‐C deficiency is predominant. The mutation, SP‐C p.V39L, was found to be relatively prevalent in China and warrants further investigation.
A Non‐Pt Electronically Coupled Semiconductor Heterojunction for Enhanced Oxygen Reduction Electrocatalytic Property
Authors: Li Fan , Qin Yong , Chalgin Aleksei , Gu Xin , Chen Wenlong , Ma Yanling , Xiang Qian , Wu Yi , Shi Fenglei , Zong Yuan , Tao Peng , Song Chengyi , Shang Wen , Deng Tao , Zhu Hong , Wu Jianbo 
Published: May 2019

AbstractHybrid faceted‐Ag3PO4/cube‐Cu2O composite materials have been fabricated and employed as oxygen reduction electrocatalysts for proton exchange membrane fuel cells (PEMFCs). The charge separation effect via the formation of PN junction has been demonstrated to boost the electrocatalysis toward oxygen reduction reaction. The as‐prepared rhombic dodecahedron‐Ag3PO4/cube‐Cu2O/C hybrid catalyst shows a mass‐specific activity of 109.80 mA/mgAg, which is about 6.4 times that of pure rhombic dodecahedron‐Ag3PO4/C catalyst (17.20 mA/mgAg). The density functional theory (DFT) calculation based on the density of states (DOS) further proved the optimal tunable effect, which is in pace with demonstration of electron transfer direction revealed by X‐ray photoelectron spectroscopy (XPS) analysis. Our work establishes a theoretical and practical basis for the rational design of newly non‐Pt hybrid catalysts, moreover, advances the future efficient application of PEMFCs.
Bimetallic Ni‐Co Silicate Hollow Spheres with Controllable Morphology for the Application on Supercapacitor
Authors: Dong Fengying , Liu Xiaojuan , Sun Xinzhi 
Published: May 2019

AbstractA facile and one‐step synthetic method is developed to produce the layered bimetallic Ni−Co silicate with the controllable morphology and porous microstructure by using the silica as the sacrifice template at the moderate reaction conditions. The bimetallic Ni−Co silicate core‐shell and hollow spheres were obtained under 140 °C for 6 h and 12 h, respectively. The pore size and the content of metal ions of the bimetallic Ni−Co silicate could be affected by the different structure. The final product shows good dispersivity, which is benefit to the homogeneity as an active material in the electrode. The as‐prepared Ni−Co silicate hollow spheres exhibit good electrochemical performance with high specific capacitance and rate capability, good charge‐discharge stability and long cycle life. The capacitance retention of the hollow structure can reach 58.69% at the current density of 10 A/g and 86.38% after 5000 cycle. The good electrochemical performance can be attributed to the different size of the pores and layer architecture. Bimetallic Ni−Co silicate hollow spheres show better electrochemical properties than the monometallic Ni or Co, which attribute to the synergistic effect.
Synthesis and Evaluation of Enalapril‐Loaded PVA/PMC Modified Magnetic Nanoparticles as a Novel Efficient Nano‐Carrier
Authors: Shahverdi Negin , Heydarinasab Amir , Panahi Homayon Ahmad , Moniri Elham 
Published: May 2019

AbstractIn the present study, the development of new Enalapril‐loaded adsorbent and evaluation of drug release was studied in vitro. Initially, Fe3O4 magnetic nanoparticles were synthesized via co‐precipitation method. Then, the mixture of polymers was deposited on the surface of the magnetic nanoparticles via radical polymerization in order to enhance the adsorption capacity of nanoparticles with biocompatible poly methylcellulose modified with polyvinyl alcohol. The equilibrium adsorption data magnetite nano‐sorbent were analyzed using Langmuir, Freundlich, and Temkin. The drug‐release study showed that 80% of Enalapril released in the simulated gastrointestinal environment for 18 hours. The samples and products analyzed by FTIR, elemental analysis, thermal gravimetric analysis (TGA) and scanning electron microscopy (SEM). Adsorption parameters such as pH, temperature and contact time were optimized, and Langmuir, Freundlich and Temkin's isotherms were studied for the drug adsorption mechanism. The release process of Enalapril studied in the simulated human digestive environment. The release profile of Enalapril has a very steep slope for 30 minutes in the simulated human gastric.
Insights into the Fluorescence Sensing Mechanism of Scandium‐Based Metal‐Organic Frameworks by Solid‐State NMR Spectroscopy
Authors: Xie Chang , Yu Zhiwu , Tong Wei , Shehzad Khurram , Xu Weihong , Wang Junfeng , Liu Jinhuai 
Published: May 2019

AbstractFluorescent metal‐organic frameworks (MOFs) have been evolved as a class of promising materials for sensing application. However, it is still a challenging issue to understand the sensing mechanism of luminescent MOFs interacting with analyte species from molecule level. Herein, fluorescent Sc2(NH2‐BDC)3 crystals are prepared as a probe. The compositional and structural characteristics of the obtained product are systematically characterized. Sc2(NH2‐BDC)3 shows admirable thermal and chemical stability in different solvents and aqueous solutions with diverse pH values. It shows excellent fluorescence sensing behaviors towards Co2+ and Ni2+ ions through fluorescence quenching effect. The sensing mechanism of Sc2(NH2‐BDC)3 towards heavy metal ions is elucidated via X‐ray photoelectron spectroscopy (XPS) analyses, Fourier transform infrared (FTIR) spectroscopy analyses, solid‐state NMR (ssNMR) spectra and electron paramagnetic resonance (EPR) analyses. The as‐prepared Sc2(NH2‐BDC)3 crystals contain some defects with formation of scandium hydroxyls in the crystal structure from the evidence analyzed by ssNMR and EPR, which are inclined to interact with Co2+ and Ni2+ ions, leading to the fluorescent quenching. This research not only demonstrates the understanding of the sensing mechanism of luminescent MOFs from molecular level, but also highlights the design of functionalized MOFs for fluorescent sensing.
Nicotine excites VIP interneurons to disinhibit pyramidal neurons in auditory cortex
Authors: Askew Caitlin E. , Lopez Alberto J. , Wood Marcelo A. , Metherate Raju 
Published: May 2019

AbstractNicotine activates nicotinic acetylcholine receptors and improves cognitive and sensory function, in part by its actions in cortical regions. Physiological studies show that nicotine amplifies stimulus‐evoked responses in sensory cortex, potentially contributing to enhancement of sensory processing. However, the role of specific cell types and circuits in the nicotinic modulation of sensory cortex remains unclear. Here, we performed whole‐cell recordings from pyramidal (Pyr) neurons and inhibitory interneurons expressing parvalbumin (PV), somatostatin (SOM), and vasoactive intestinal peptide (VIP) in mouse auditory cortex, in vitro. Bath application of nicotine strongly depolarized and excited VIP neurons, weakly depolarized Pyr neurons, and had no effect on the membrane potential of SOM or PV neurons. The use of receptor antagonists showed that nicotine's effects on VIP and Pyr neurons were direct and indirect, respectively. Nicotine also enhanced the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) in Pyr, VIP, and SOM, but not PV, cells. Using Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), we show that chemogenetic inhibition of VIP neurons prevents nicotine's effects on Pyr neurons. Since VIP cells preferentially contact other inhibitory interneurons, we suggest that nicotine drives VIP cell firing to disinhibit Pyr cell somata, potentially making Pyr cells more responsive to auditory stimuli. In parallel, activation of VIP cells also directly inhibits Pyr neurons, likely altering integration of other synaptic inputs. These cellular and synaptic mechanisms likely contribute to nicotine's beneficial effects on cognitive and sensory function.