bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Climatic Change on the Current and Future Niche of Zoonotic Cutaneous Leishmaniasis Vector and Reservoir Species in Yazd Province
Authors: Shiravand Babak , Dehghani Tafti Abbas Ali , Mousavi Seyed Hossein , Taj Firouzeh Ali Akbar , Hosseini Seyed Akbar 
Published: September 2019

Introduction: Leishmania tropica and Leishmania major are the causative agents of Anthroponotic Cutaneous Leishmaniasis (ACL) and Zoonotic Cutaneous Leishmaniasis (ZCL) in various areas of Iran, respectively. Yazd province is one of the endemic centers of ZCL. Therefore, this study aimed to investigate and predict the effects of the climate change on the potential scattering of the vector and reservoir species of ZCL disease in Yazd province, as one of the endemic centers of ZCL in Iran in the present (1950-2000) and prospective time (2030).
Materials and Methods: According to the findings of prior studies conducted in Yazd province, the data related to the vectors and reservoir of the ZCL were collected and recorded in the databank. ArcGIS 10.3 and MaxEnt software were used to estimate the suitable ecological niches using 19 variables. In this study, Beijing Climate Center Climate System Model and scenario of Representative Concentration Pathway 4.5 were applied with respect to 2030 horizon.
Results: According to results of Jackknife test, the climate variables of Bio8 & Bio6 for the current period, and climate variables of Bio8 & Bio7 for the future (2030) produced the most effects on the distribution of vector and reservoir species in Yazd province. These results indicated that temperature had the greatest impact on the vector’s distribution in the present and future. Currently, eastern and central areas of the province are more likely to receive most of the vector and reservoir species. It is anticipated that in the future, we will observe an increase in the presence of vector in the western districts and reservoirs in the northern and central regions of the province.
Conclusion: Climate conditions provide suitable habitats for easy transfer of ZCL disease in Yazd province. This research confirmed that suitable climatic conditions for the vector and reservoir will be expanded in coming years in comparison with the current period.
APLIKASI CUTI DOSEN BERBASIS ANDROID PADA STEBIS ISLAM DARUSSALAM
Authors: Solikin Imam , Putra M. Soekarno 
Published: September 2019

<p><em>The development of information technology is currently growing which makes it easier for users with a mobile or android based system that can be used wherever and whenever the user wants. Bina Darma University Palembang is one of the private universities in Palembang that has been accredited B and has ISO standards. Bina Darma University has employees including employees and staff and lecturers. At Bina Darma University Palembang the process of filing leave is still done manually. Employees and lecturers who will propose leave are required to make and provide a leave of reference letter. By using this android-based leave application, it makes it easier for lecturers or employees to carry out the process of filing leave, because it can be accessed anywhere and anytime so that it can improve the time in filing leave.</em></p><p><em><strong>Keywords</strong></em><em>: Android, Leave, Lecturer</em> </p><p><em>Perkembangan teknologi informasi saat ini yang semakin berkembang yang semakin memudahkan pengguna dengan adanya sistem berbasi mobile atau android yang dapat digunakan dimanapun dan kapanpun pengguna inginkan. Universitas Bina Darma Palembang merupakan salah satu Universitas swasta di kota Palembang yang telah terakreditasi B dan berstandar ISO. Universitas Bina Darma memiliki pegawai yang meliputi karyawan beserta staf dan dosen pengajar. Pada Universitas Bina Darma Palembang proses pengajuan cuti masih dilakukan secara manual. Karyawan dan dosen yang akan mengajukan cuti yaitu harus membuat dan memberikan surat pengacuan cuti. Dengan menggunakan aplikasi pengajuan cuti berbasis android ini memudahkan dosen ataupun karyawan dalam melakukan proses pengajuan cuti, karena dapat diakses dimanapun dan kapanpun sehingga hal ini dapat mengefienkan waktu dalam pengajuan cuti.</em></p><p><em><strong>Kata kunci</strong></em><em>: Android, Cuti, Dosen</em></p>
Creation and characterisation of 11β hydroxysteroid dehydrogenase type 1 transgenic mesenchymal stem cells for use in models of acute respiratory distress syndrome
Authors: MAHIDA RAHUL Y. , Yuan Zhengqiang , Kolluri Krishna K. , Lugg Sebastian T. , Scott Aaron , Parekh Dhruv , Hardy Rowan S. , Lavery Gareth G. , Janes Sam M. , Thickett David R. 
Published: September 2019

Abstract
Background: Human bone marrow mesenchymal stem cell (MSC) administration reduces inflammation in pre-clinical models of lung injury, however clinical efficacy in patients with acute respiratory distress syndrome (ARDS) has not been shown. Upregulation of the glucocorticoid activating enzyme, 11β hydroxysteroid dehydrogenase type 1 (HSD-1) within the alveolar space elevates local anti-inflammatory cortisol levels, and promotes alveolar macrophage efferocytosis. Administration of HSD-1 transgenic MSCs (tMSCs) that overexpress HSD-1 may enhance local cortisol activation. This combined cellular and gene therapy may be more effective at reducing inflammation in ARDS than cellular therapy alone. Methods: Molecular cloning was used to create a recombinant lentiviral vector containing the HSD-1 and green fluorescent protein (GFP) transgenes. MSCs were transfected using this lentivirus, then transfection efficiency was assessed using flow-cytometry. HSD-1 transgene expression was assessed using immunofluorescence and western blotting. Thin layer chromatography was used to assess HSD-1 function in tMSCs. Bi-lineage differentiation and flow-cytometry were used to determine whether tMSCs maintained a stem cell phenotype. Results: A recombinant lentiviral vector was created containing the HSD-1 and GFP transgenes under the control of a tetracycline promoter. MSCs were successfully transfected, with a transfection efficiency of 91.1%. HSD-1 transgene expression was confirmed by immunofluorescence and western blot. Functional HSD-1 activity was evident within tMSCs, with predominant reductase cortisol activation, following treatment with the transcriptional activator doxycycline. HSD-1 reductase activity was maintained for 72 hours after doxycycline was removed from tMSCs. HSD-1 tMSCs maintained the capacity for osteogenic and adipogenic differentiation, and maintained expression of MSC surface markers. Conclusions: We successfully transduced tMSCs to express the HSD-1 transgene that functions predominantly as a reductase. Administration of HSD-1 tMSCs to in vivo models of ARDS may attenuate inflammation through activation of cortisol in the alveolar space.
Identification of catechol dioxygenases of acinetobacter species, with improved ability to degrade crude oil
Authors: AL-Kadmy Israa M.S. , Suhail Ahmed , Abid Suhad Abbas , Aziz Sarah Naji , Abed Al-Maamoon H. , Al-sallami Karrar Jasim 
Published: September 2019

Abstract
Background: Various microbes involved in alkanes degradation or reducing organics oil products have been identified among. The goal of this work was to screen the soil bacteria with potential ability to degrade alkanes and characterized using a combination of genetic and physiological methods.Methods: The microbial species of soil sample was isolated and the loss of hydrocarbon was calculated periodically by gravimetric analysis. Isolated bacterial strain with potential ability to degrade the engine oil in Baghdad city was further tested for hydrocarbon-degrading abilities using 2, 6-DCPIP. Colonies of bacteria were counted using serial dilution methods of the soil sample. One of the highly bioactive degrading strains ISN53 was selected and a different fraction of crude extract were analyzed for their hydrocarbon degrading activity. The purified active fraction characterised in LC/MS and SDS PAGE. Presence of chlorocatechol dioxygenases was checked by PCR and expression was analyzed in RT PCR. The envelope of ISn21 isolate was checked for any changes under different conditions using microbial adhesion to the hydrocarbon test (MATH). Results: Serratia marcesens (Sm53) and Acinetobacter venetianus (ISn21) were isolated and ISn21 showed maximum growth on the third day and up to 72% degradation on the 7th day of incubation. LC-MS/MS spectrometry analysis identified the bioactive enzyme catechol dioxygenases in crude extract. The purified catechol dioxygenases enzyme had 35 kDa weight which showed the maximum activity between 48 to 72 hour of incubation. The expression of its gene was 2 fold more than control the maximum expression was observed at 60 to 72 hours of incubation. ISn21 was extremely hydrophobic both in MMV and in LB medium.Conclusion: Using a naturally available resources would be better with efficiency at degrading diesel. Use of like microorganisms during bioremediation operation might supply worthy advances in this important biotechnological/ biological domain.
Prevalence of impaired vision and associated factors among primary school children in primary schools of Addis Ababa, central Ethiopia
Authors: Hailu Yemisrach , Hiko Desta , Shaweno Tamrat 
Published: September 2019

Abstract
Background Visual impairment refers to reduction of vision resulting in a lower than normal visual acuity. Globally, approximately 1.3 billion adults and children live with some form of vision impairment. Visual impairment is usually asymptomatic, overlooked and affects school performance and other functions. School programs are recommended for early detection and timely interventions. In resource limited countries like Ethiopia, undetected visual impairment, lack of adequate and up-to-date information on the problem remain as huge gaps. Thus, we assessed the prevalence of visual impairment (VI), and the associated factors among children attending government primary schools of Lideta sub-city, Addis Ababa, Ethiopia.Methods This study was a s chool based cross-sectional study conducted from April to May 2019, using a two staged-cluster sampling method in Lideta sub-city, Addis Ababa, Ethiopia. From the total of 18 government primary schools in Lideta sub-city, 6 were selected using probability proportionate to size (PPS) method. The study population was primary school children aged 7 to 17 in the selected 6 primary schools. A total of 816 primary school children were included in the study. The selected children have undergone screening for the presence of visual impairment and other eye problems. Children’s parents/guardians were interviewed using structured pre-tested questionnaires. Epi-data3.1 and SPSS version 20 were used for data entry and analysis, respectively. Binary logistic regression was performed to check association between dependent and independent variables. Significance was set at p-value <0.05.Results A total of 773 children were examined for visual impairment. Out of the 773 children, 370 (47.9%) were males and 403(52.1) were females with age range 7 to 17 years and the mean age being 11.69 (SD 2.64). The prevalence of impaired vision among school based children was 4.4%. The causes of visual impairment included: Myopia (43%), Astigmatism with or without Amblyopia (31%), Hyperopia with or without Amblyopia (20%) and others. The students not being aware of eye problem (AOR=2.2, 95%CI: 1.08-4.35) was only significantly associated factor with visual impairment.Conclusion Visual impairment is a public health concern, the causes are treatable. Regular school screening programs and affordable treatment should be available.
Flipped classroom improves results in pathophysiology learning: results of a non-randomised controlled study.
Authors: Herrero J. Ignacio , Quiroga Jorge 
Published: September 2019

Abstract
Background. The flipped classroom has become increasingly popular in health professions education. The aim of this study was to analyse its effect on learning in a pathophysiology course.
Methods. Flipped classroom was introduced to teach respiratory pathophysiology in 2018. We compared exam results with the results the previous year and with the exam results from blood pathophysiology from both years (taught by the same teacher, in a traditional way). Groups were compared with the Student’s T test. Students answered a survey after finishing the term.
Results. 201 students were examined in 2018 (and 229 in 2017). Gender distribution and the qualifications obtained in general pathology were comparable in both years. The results in respiratory pathophysiology were significantly better in 2018 than in 2017 (mean: 48 versus 42 out of 100; P=0.004), but the results in blood pathophysiology remained similar. The improvement was significant only in students that scored below the median (mean: 40 versus 33; P=0.009) and was more evident in male than in female students (mean: 52 versus 44; P=0.010) and in those who had the same age as (were not older than) the rest of their classmates (mean 51 versus 44; P=0.002). Most students considered that flipped classroom was more attractive and helped them to learn more and with less effort.
Conclusions. Flipped classroom increases medical students’ knowledge acquisitions in pathophysiology. It is more beneficial to male students and those with lower qualifications without academic delay.
Viability determination of Ascaris ova in raw wastewater: a comparative evaluation of culture-based, BacLight Live/Dead staining and PMA-qPCR methods
Authors: Ravindran Vivek B. , Shahsavari Esmaeil , Soni Sarvesh K. , Ball Andrew S. 
Published: September 2019

Abstract
Accurate evaluation of viable Ascaris ova in wastewater is the key to mitigating Ascaris reinfections in endemic regions. In this study, the viability of Ascaris ova in raw wastewater was determined using three different detection methods: culture-based, BacLight Live/Dead staining and propidium monoazide–quantitative polymerase chain reaction (PMA-qPCR). Furthermore, comparative assessment of viability utilising the aforementioned detection methods was performed using seeded experiments in wastewater. The percentage of viability was: culture-based (82%), BacLight Live/Dead staining (87%) and PMA-qPCR (85%) respectively. Despite the fact that no statistical difference was shown in the viability determination among the three methods, PMA-qPCR-based viability determination would be preferable over the other two methods for evaluating potential public health risks with A. suum ova due to its accuracy, being least subjective and its rapid reaction time.
Methotrexate in Early Chikungunya Arthritis: A 6 Month Randomized Controlled Open-label Trial
Authors: Adarsh M.B. , Sharma Shefali K. , Dwivedi Preksha , Singh Mini P. , Dhir Varun , Jain Sanjay 
Published: September 2019

Objective:
Evidence for treating chikungunya arthritis early in the course of illness is
scarce. This study assesses the efficacy of Methotrexate in early Chikungunya arthritis.

Methods:
It is a randomized controlled open-label assessor-blinded trial with a crossover design.
Sixty patients with persistent post chikungunya arthritis with at least 3 or more tender or swollen
joints (28 joint count) were recruited. MTX arm was given oral Methotrexate and NSAID arm was
given NSAIDs (Naproxen 1 gm/day or Etoricoxib 120 mg/day). Patients were followed at 1, 2, 4
and 6 months. After 2 months patients in NSAID arm who have not achieved remission were given
MTX. The primary endpoint was remission (no tender or swollen joints by 28 joint count) at 6
months. Secondary endpoints were change in CDAI, Indian HAQ, total steroid use, total NSAID
use, and serious adverse effects. Intention to treat analysis was used.

Results:
TJC, SJC, CDAI and HAQ were matched between two at baseline. Remission was
achieved by 28 patients (93%, CI- 78%-98%) in the NSAID arm and 26 patients (86%, CI-70%-
94%) in MTX arm (p=0.18). There was no significant difference in steroid need, change in HAQ,
CDAI, TJC or SJC. Those who have not achieved remission had higher disease activity at baseline.

Conclusion:
A protocol-based approach with steroid and NSAIDs helped to achieve remission in
most patients with early subacute phase of post-Chikungunya arthritis and the effect was comparable
to that of early initiation of methotrexate.
Endodontic Management of Hypercementosis in Conjunction with Asymptomatic Apical Periodontitis: A Case Report
Authors: Heredia Gabriela E.I. , Morales Santiago H.M. , Niño Juan C.M. , Gutmann James L. 
Published: September 2019

Introduction:
Hypercementosis is characterized by an excessive deposit of dental cementum. While it is considered a benign pathology, it can sometimes be associated with a chronic pulpal or periapical infection. Furthermore, its management is complex, because establishing an accurate apical limit for a root canal procedure is not always possible.

Methods:
When a periapical lesion is present, a conservative, surgical approach to remove the inflammatory tissue from the periapical lesion can be considered as a treatment option.

Conclusion:
This paper reports on the management of a tooth with hypercementosis accompanied by an asymptomatic apical periodontitis affecting a left mandibular second molar.
Scalable Manufacturing Processes for Solid Lipid Nanoparticles
Authors: Anderluzzi Giulia , Lou Gustavo , Su Yang , Perrie Yvonne 
Published: September 2019

Background:
Solid lipid nanoparticles offer a range of advantages as delivery
systems but they are limited by effective manufacturing processes.

Objective:
In this study, we outline a high-throughput and scalable manufacturing process
for solid lipid nanoparticles.

Method:
The solid lipid nanoparticles were formulated from a combination of tristearin
and 1,2-Distearoyl-phosphatidylethanolamine-methyl-polyethyleneglycol conjugate-2000
and manufactured using the M-110P Microfluidizer processor (Microfluidics Inc, Westwood,
Massachusetts, US).

Results:
The manufacturing process was optimized in terms of the number of process cycles
(1 to 5) and operating pressure (20,000 to 30,000 psi). The solid lipid nanoparticles
were purified using tangential flow filtration and they were characterized in terms of their
size, PDI, Z-potential and protein loading. At-line particle size monitoring was also incorporated
within the process. Our results demonstrate that solid lipid nanoparticles can be effectively
manufactured using this process at pressures of 20,000 psi with as little as 2 process
passes, with purification and removal of non-entrapped protein achieved after 12 diafiltration
cycles. Furthermore, the size could be effectively monitored at-line to allow rapid
process control monitoring and product validation.

Conclusion:
Using this method, protein-loaded solid lipid nanoparticles containing a low
(1%) and high (16%) Pegylation were manufactured, purified and monitored for particle
size using an at-line system demonstrating a scalable process for the manufacture of these
nanoparticles.
MODEL PENJAMINAN MUTU PEMBELAJARAN PADA PERGURUAN TINGGI SWASTA
Authors: Supriyanto Eko 
Published: September 2019

Most of Muhammadiyah universities in specific region of Yogyakarta have not implemented yet the quality assurance, exactly it demands both in the society and government that cannot be avoided . Therefore, this study aims to find a specific model of quality assurance in the university environment Muhammadiyah as an attempt to answer the demands in this atas. This research is qualitative research with a target of six colleges in the Muhammadiyah state uni- versities in specific region of Yogyakarta and Central Java. Those are Muhammadiyah University of Yogyakarta, University of Ahmad Dahlan, Muhammadiyah University of Magelang, Muham- madiyah University of Surakarta, Muhammadiyah University of Purworejo and Muhammadiyah University of Purwokerto. The procedure adopted to find the model of quality assurance through theoretical studies, collecting data from informants about the aspects that should be subjected to quality assurance, modeling and verification of quality assurance models. Data collection was carried out with questionnaire, interviews and documentation. Data analysis was performed with a description of the data, the analysis of flows (Flow Analysis), and contingency. The results of this study consist of two models: Model for quality assurance in learning and quality assurance models for lecturers. The forms of quality assurance is a combination of a standard element of the quality of academic and ideologies of Muhammadiyah, while the quality assurancer are the Muhammadiyah Central Leadership, Daily Executive Agency (BPH) and the Directorate General of Higher Education. The quality assurance models found through networking opinion, in-depth interviews and document the aspects that should be subject to quality assurance to college Mu- hammadiyah Yogyakarta and Central Java. The advantages of the quality model assurance are efficiency in cost and simple in the tools and mechanism that fit to the character of Muhammadi- yah state universities. The mechanism of the model is done by committed leadership begins, then formulate the quality standards by the elements of quality assurance later implemented through institutional self-assessment and faculty independently. The development and ideology of the aca- demic community is done through the assessment.
EFEKTIFITAS PEMBELAJARAN KETERAMPILAN SPEAKING DENGAN PENDEKATAN GENRE DI UNIVERSITAS MUHAMMADIYAH SURAKARTA
Authors: Slamet Sri 
Published: September 2019

The purposes of this research are to describe language teaching using genre approach, to describe the problems faced by students and lecturers at Language Center UMS, and to de- scribe the strengths and weaknesses of this approach in which the approach is compared with a previous method. This research is a descriptive qualitative research. It uses informant and docu- ment sources. The informants are the students of semester 2 and the lecturers of Speaking Acqui- sition 2 at UMS. The techniques used in data collecting are by giving questionnaire, doing an in- terview, observing, and giving a test. The result of the research shows that (1) teaching English using genre approach has not been effective yet. The ineffectiveness of this approach is caused by (a) the lecturers, who still do not understand the concept of genre and how to teach it to the stu- dents, (b) the topic of dialogue is not interesting to the students, (c) the exercises of the book are not various, and (d) the students are not well-motivated by the lecturers. (2) Genre approach in teaching English is one of the methods to develop student and lecturer’s activity on speaking abil- ity. (3) The weakness of this approach is that the students are still lack of mastery on vocabulary, grammar and pronunciation. Based on the finding, it is suggested for the policy maker at LC to hold a workshop on teaching Speaking based on genre approach. The materials on workshop should be focused on teaching speaking for a big class, the method on giving the task or assign- ment, and the ways to evaluate it. It is also suggested that the curriculum should cover Reading Subject which can be used to emphasize on students vocabulary, grammar, and pronunciation.
Bertolaso Stella na fronteira entre teologia e ciência da religião: um exercício de religiografia na pré-história da autonomia de duas áreas
Authors: Calvani Carlos Eduardo 
Published: September 2019

O artigo apresenta uma pesquisa historiográfica baseada em fontes pouco conhecidas da pré-história da Ciência da Religião no Brasil, em um esforço religiográfico de compreender uma época em que as fronteiras entre Teologia e Ciência da Religião ainda eram muito incertas. A pesquisa recupera fontes documentais primárias (artigos acadêmicos e publicações autofinanciadas), testemunhos escritos de pesquisadores já falecidos ou aposentados e um breve registro de história oral a respeito dos esforços do pastor presbiteriano Jorge Bertolaso Stella que, entre os anos de 1930 e 1970, debruçou-se no estudo de diversos temas que não faziam parte da agenda de pesquisadores brasileiros das universidades da época.