bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interpersonal needs and suicide risk: The moderating roles of sex and brooding
Authors: Lear Mary K. , Kozina Ryan M. , Stacy Stephanie E. , Clapp Joshua D. , Pepper Carolyn M. 
Published: May 2019

AbstractObjectivesThis paper tested two moderators, brooding and participant sex, on the respective relations between thwarted belongingness (TB), perceived burdensomeness (PB), and suicide risk.MethodUsing a cross‐sectional design in a sample of undergraduates (N = 278), two hierarchical regression models examining the three‐way interaction between brooding, sex, and either TB or PB on suicide risk were conducted.ResultsA significant two‐way interaction of brooding and TB was detected, but no moderating effect of sex was observed. There was a significant three‐way interaction of brooding, sex, and PB on suicide risk.ConclusionsHigh levels of TB and brooding produce increased suicide risk across sexes. The relation between PB and suicide risk is dependent on brooding for females but not males.
Retracted: Upregulated microRNA‐15b alleviates ovarian cancer through inhitbition of the PI3K/Akt pathway by targeting LPAR3
Authors: Li Guang‐Cai , Qin Xu‐Ling , Song Huai‐Hua , Li Ying‐Ni , Qiu Yu‐Yan , Cui Shi‐Chang , Wang Yong‐Sheng , Wang Hui , Gong Jun‐Ling 
Published: May 2019

AbstractOvarian cancer characterizes as the fourth leading consequence of death associated with cancer for women. Accumulating evidence underscores the vital roles of microRNAs (miRNAs) in preventing ovarian cancer development. Besides, induction of the phosphatidylinositol‐3 kinase/serine/threonine kinase (PI3K/Akt) pathway associated with the ovarian cancer cell migration and invasion. The study aims to examine the effects of miR‐15b on the proliferation, apoptosis, and senescence of human ovarian cancer cells by binding to lysophosphatidic acid receptor 3 (LPAR3) with the involvement of the PI3K/Akt pathway. The positive expression of LPAR3 protein was detected by immunohistochemistry. Then the interaction between miR‐15b and LPAR3 was examined. The possible role of miR‐15b in ovarian cancer was explored using gain‐ and loss‐of‐function experiments. Subsequently, the functions of miR‐15b on PI3K/Akt pathway, proliferation, migration, invasion, senescence and apoptosis of ovarian cancer cells were assessed. Furthermore, in vivo tumorigenicity assay in nude mice was performed. LPAR3 was overexpressed, whereas miR‐15b was poorly expressed in ovarian cancer tissues. LPAR3 is a direct target of miR‐15b. Restored miR‐15b promoted Bax expression, apoptosis, and senescence, inhibited expression of LPAR3 and Bcl‐2, the extent of PI3K and Akt phosphorylation, as well as ovarian cancer cell proliferation, migration, and invasion. Further, tumor growth was observed to be prevented by miR‐15b overexpression. Collectively, our study demonstrates that miR‐15b represses the proliferation and drives the senescence and apoptosis of ovarian cancer cells through the suppression of LPAR3 and the PI3K/Akt pathway, highlighting an antitumorigenic role of miR‐15b.
Systematic analysis of alternative splicing signature unveils prognostic predictor for kidney renal clear cell carcinoma
Authors: Song Jukun , Liu Yong Da , Su Jiaming , Yuan Dongbo , Sun Fa , Zhu Jianguo 
Published: May 2019

AbstractThere is growing evidence that alternative splicing (AS) plays an important role in cancer development. However, a comprehensive analysis of AS signatures in kidney renal clear cell carcinoma (KIRC) is lacking and urgently needed. It remains unclear whether AS acts as diagnostic biomarkers in predicting the prognosis of KIRC patients. In the work, gene expression and clinical data of KIRC were obtained from The Cancer Genome Atlas (TCGA), and profiles of AS events were downloaded from the SpliceSeq database. The RNA sequence/AS data and clinical information were integrated, and we conducted the Cox regression analysis to screen survival‐related AS events and messenger RNAs (mRNAs). Correlation between prognostic AS events and gene expression were analyzed using the Pearson correlation coefficient. Protein‐protein interaction analysis was conducted for the prognostic AS‐related genes, and a potential regulatory network was built using Cytoscape (version 3.6.1). Meanwhile, functional enrichment analysis was conducted. A prognostic risk score model is then established based on seven hub genes (KRT222, LENG8, APOB, SLC3A1, SCD5, AQP1, and ADRA1A) that have high performance in the risk classification of KIRC patients. A total 46,415 AS events including 10,601 genes in 537 patients with KIRC were identified. In univariate Cox regression analysis, 13,362 survival associated AS events and 8,694 survival‐specific mRNAs were detected. Common 3,105 genes were screen by overlapping 13,362 survival associated AS events and 8,694 survival‐specific mRNAs. The Pearson correlation analysis suggested that 13 genes were significantly correlated with AS events (Pearson correlation coefficient >0.8 or <−0.8). Then, We conducted multivariate Cox regression analyses to select the potential prognostic AS genes. Seven genes were identified to be significantly related to OS. A prognostic model based on seven genes was constructed. The area under the ROC curve was 0.767. In the current study, a robust prognostic prediction model was constructed for KIRC patients, and the findings revealed that the AS events could act as potential prognostic biomarkers for KIRC.
Transcriptome analysis reveals lncRNA‐mediated complex regulatory network response to DNA damage in the liver tissue of Rattus norvegicus
Authors: Huang Chen , Leng Dongliang , Lei Kuan Cheok , Sun Shixue , Zhang Xiaohua Douglas 
Published: May 2019

AbstractDNA is prone to damages, which would result in genetic disorders and enhance risk of tumorigenesis. Hence, understanding the molecular mechanisms of DNA damage and repair will provide deep insights into tumorigenesis, carcinogenesis as well as the corresponding treatments. Aiming at investigating potential long noncoding RNAs (lncRNAs) response against DNA damage, we performed a comprehensive transcriptomic analysis based on RNA sequencing data of the liver tissue from Rattus norvegicus, in which DNA damage was induced using aflatoxin B1, ifosfamide and N‐nitrosodimethylamine. Through our analyses, numerous novel lncRNAs are identified for the first time, and differential network analysis discloses lncRNA‐mediated regulatory networks related to DNA‐damage response. The result shows that these DNA‐damage‐inducing chemicals might disrupt many lncRNA‐mediated interactions involved in diverse biological processes and pathways, for example, immune function and cell adhesion. In contrast, the host might also activate a few RNA interactions in response to DNA damage, involving response to drug and regulation of cell cycle.
Cardioprotective role of extracellular vesicles: A highlight on exosome beneficial effects in cardiovascular diseases
Authors: Rezaie Jafar , Rahbarghazi Reza , Pezeshki Milad , Mazhar Mahdi , Yekani Farshid , Khaksar Majid , Shokrollahi Elhameh , Amini Hassan , Hashemzadeh Shahriar , Sokullu Sadiye Emel , Tokac Mehmet 
Published: May 2019

AbstractExtracellular vesicles (EVs) are nano‐sized vesicles, released from many cell types including cardiac cells, have recently emerged as intercellular communication tools in cell dynamics. EVs are an important mediator of signaling within cells that inﬂuencing the functional behavior of the target cells. In heart complex, cardiac cells can easily use EVs to transport bioactive molecules such as proteins, lipids, and RNAs to the regulation of neighboring cell function. Cross‐talk between intracardiac cells plays pivotal roles in the heart homeostasis and in adaptive responses of the heart to stress. EVs were released by cardiomyocytes under baseline conditions, but stress condition such as hypoxia intensifies secretome capacity. EVs secreted by cardiac progenitor cells and cardiosphere‐derived cells could be pinpointed as important mediators of cardioprotection and cardiogenesis. Furthermore, EVs from many different types of stem cells could potentially exert a therapeutic effect on the damaged heart. Recent evidence shows that cardiac‐derived EVs are rich in microRNAs, suggesting a key role in the controlling of cellular processes. EVs harboring exosomes may be clinically useful in cell‐free therapy approaches and potentially act as prognosis and diagnosis biomarkers of cardiovascular diseases.
Histology and ultrastructure of the testis and vas deferens in Pseudochorthippus parallelus parallelus (Orthoptera, Acrididae)
Authors: Polat Irmak , Amutkan Mutlu Damla , Ünal Mustafa , Suludere Zekiye 
Published: May 2019

Abstract
Pseudochorthippus parallelus parallelus (Zetterstedt, 1821) (Orthoptera, Acrididae) is a widespread species in Europe, and also it is localized in some regions in Turkey such as Bursa, Eskişehir, Ankara, Bolu, Düzce, and Çankırı. The features of the reproductive organs such as the numbers and shapes of testes and follicles can be used as taxonomical characters. For this purpose, the ultrastructural and histological features of testis and vas deferens in P. parallelus parallelus were examined with using light microscope, scanning electron microscope, and transmission electron microscope. The mature P. parallelus parallelus has two conjugated testes produce spermatozoa. Each testis is composed of numerous testis follicles in which different stages of spermatogenesis and spermiogenesis develop. First, spermatocytes are formed by the mitosis division of the germ cells at the distal end of the follicles. Then, spermatocytes form spermatids by meiosis division in the middle region of the follicles. Finally, spermatids are differentiated to spermatozoa at the proximal region of the follicles. After maturation of the spermatozoa, sperm tails come together as the sperm bundles called as spermatodesm. Each follicle is connected to vas deferens via vas efferens to discharging spermatozoa. In spite of some differences, the testes and the vas deferens in P. parallelus parallelus are highly similar to the those of other species, especially Orthopteran species.
Analytical modeling of 2DEG with 2DHG polarization charge density drain current and small‐signal model of quaternary AlInGaN HEMTs for microwave frequency applications
Authors: N. Anbuselvan , N. Mohankumar , A. Mohanbabu 
Published: May 2019

AbstractA physics‐based analytical model for quaternary AlInGaN high electron mobility transistors (HEMTs) is developed including two‐dimensional electron gas (2DEG) and two‐dimensional hole gas (2DHG) for microwave applications. The DC and RF performance characteristics are explored by considering the quasi‐triangular quantum well (QW). The derived charge carrier densities nsand psare considered for energy subbands Eo and E1 inside QW. The 2DEG sheet carrier concentration density remains constant as long as 2DHG exists. From the derived model, the drain current (Id), transconductance current gain (gm), and cutoff frequency (ft) for different gate length and width are verified with experimental data for upcoming nano‐scale devices.
Development and evaluation of gastroretentive ciprofloxacin floating tablets using Chrysophyllum albidum gum
Authors: Bakre Lateef G. , Ladele Boluwatife A. 
Published: May 2019

AbstractBackgroundThe floating drug delivery system offers immense advantages for the controlled delivery of drugs because it increases gastric residence time and thereby improves drug bioavailability.AimThe aim of this study was to design a gastroretentive drug delivery system for controlled delivery of ciprofloxacin using Chrysophyllum albidum gum (CAG) in combination with hydrophilic polymers such as xanthan gum (XG) and hydroxypropyl methylcellulose (HPMC).MethodsFloating tablets prepared by the effervescence method and direct compression were evaluated for hardness, friability, swelling index, floating lag time and total floating time. In vitro drug release data were fitted into different drug release kinetic models, and the mechanism of drug release was determined.ResultsThe tablets exhibited satisfactory physicochemical characteristics, showed good in vitro buoyancy and swelled considerably. Tablets with a higher amount of CAG floated for a longer duration and had more matrix integrity than formulations containing lower amounts of CAG. The optimised formulation exhibited 50% and 90% drug release in 6.5 and 11.5 h, respectively, which suggests a sustained release effect, whereas the buoyancy lag time was 18 s. Analysis of the in vitro release kinetics suggests that the drug was released from the optimised formulation by diffusion, and the drug release mechanism was observed to be Fickian diffusion.ConclusionThis study highlights the potential of a ciprofloxacin floating system prepared from xanthan gum, HPMC and CAG as a suitable alternative to the conventional dosage and other sustained‐release formulations.
Raman study of amorphization in nanocrystalline 3C–SiC irradiated with C+ and He+ ions
Authors: Zhang Limin , Jiang Weilin , Pan Chenglong , Fadanelli Raul C. , Ai Wensi , Chen Liang , Wang Tieshan 
Published: May 2019

AbstractThis study examines C+ and He+ ion irradiation‐induced amorphization processes in 3C–SiC nanograins embedded in an amorphous SiC matrix. Raman spectroscopy and Rutherford backscattering spectrometry are used for damage characterization. SiC grains with an average size of either ~6 or ~20 nm were observed to be fully amorphized to a lower dose at room temperature compared with their monocrystalline counterpart under the identical irradiation condition. In addition to damage accumulation‐induced amorphization in the grains, preferential amorphization at the crystalline/amorphous interfaces could play a significant role. This interface‐driven amorphization proceeds at comparable rates for C+ and He+ ion irradiations. The results may have an important implication for nanocrystalline SiC to be applied in advanced nuclear reactors.
In vivo contrast‐enhanced microCT for the monitoring of mouse thoracic, lumbar, and coccygeal intervertebral discs
Authors: Walk Remy E. , Tang Simon Y. 
Published: May 2019

AbstractMouse models are often used for studies of intervertebral disc (IVD) homeostasis and degeneration, yet the relatively small size of the IVD poses challenges for noninvasive, longitudinal imaging modalities. The recently developed contrast‐enhanced microCT (CEμCT) using Ioversol has been successful in detecting degenerative changes in the murine IVD ex vivo at the micrometer scale. Further leveraging the superior biocompatibility of Ioversol as a contrast agent, we demonstrate the in vivo use of this CEμCT technique to examine IVDs at multiple spinal sites. Ioversol was administered via tail vein injection (TVI) in growing and adult male FVB/NJ mice (n = 5 /group). The animals were anesthetized and underwent in vivo micro‐computed tomographic (microCT) at the coccygeal (CC5/CC6), lumbar (L5/6), and thoracic (T12/T13) IVDs. TVI of Ioversol was well‐tolerated by all animals. As Ioversol filtered through the kidneys and accumulated in the bladder, the attenuations of the mouse bladder and kidneys increased due to the high molecular weight of Ioversol, confirming that the Ioversol is biological available. Average IVD attenuations increased 3%‐15% following TVI (ANOVA; P < .01). The presence of Ioversol in the IVD combined with high‐resolution microCT allow for nondestructive visualization of structural features of the IVD. These results demonstrate CEμCT with Ioversol as a viable strategy for the in vivo monitoring of multiple mouse IVDs during degeneration, disease, and injury.
Network Analysis of Posttraumatic Stress Experiences of Adults Seeking Psychological Treatment for Childhood Sexual Abuse
Authors: McBride Orla , Hyland Philip , Murphy Jamie , Elklit Ask 
Published: May 2019

AbstractNetwork analysis proposes that mental disorders may best be construed as causal systems embodied in networks of functionally interconnected symptoms. We employed network analysis to test how adult survivors of childhood sexual abuse (CSA) experienced symptoms of posttraumatic stress, using alternative conceptualizations of posttraumatic stress disorder (PTSD). Given the characteristics of the sample (i.e., the nature of and time since trauma), we hypothesized that (a) symptoms related to arousal would not be prominent in the networks and (b) symptoms related to negative alternations in cognition and mood (NACM) would be core components in the network. Danish adults seeking psychological treatment for CSA (n= 473) completed the Harvard Trauma Questionnaire and Trauma Symptom Checklist. Three alternative models (DSM‐5,DSM‐5with dissociation, andICD‐11complex PTSD [CPTSD]) were estimated using regularized partial correlation models. In theDSM‐5network, strong associations emerged for experiences of NACM (blame and guilt) and intrusions (thoughts and flashbacks). The addition of “depersonalization” and “derealization” to theDSM‐5model produced a strong association, but these experiences were largely unrelated to other PTSD clusters. In the CPTSD network, interpersonal problems and negative self‐concept were central to the survivors’ experiences. For this highly‐specific survivor group who experienced traumatic CSA many years ago, experiences related to NACM appeared to be more central to the posttrauma experience than those of arousal. If replicated, these findings could help inform treatment plans for specific groups of survivors. Methodological implications as to the usefulness of network models in the psychopathological research literature are discussed.
Mannose‐Modified Serum Exosomes for the Elevated Uptake to Murine Dendritic Cells and Lymphatic Accumulation
Authors: Choi Eun Seo , Song Jihyeon , Kang Yoon Young , Mok Hyejung 
Published: May 2019

AbstractThe surface of bovine serum‐derived exosomes (EXOs) are modified with α‐d‐mannose for facile interaction with mannose receptors on dendritic cells (DCs) and for efficient delivery of immune stimulators to the DCs. The surface of the EXOs is modified with polyethylene glycol (PEG) without particle aggregation (≈50 nm) via the incorporation of 1,2‐distearoyl‐sn‐glycero‐3‐phosphoethanolamine (DSPE) into the lipid layer of the EXO, compared to chemical conjugation by N‐hydroxysuccinimide activated PEG (NHS‐PEG). PEG modification onto the exosomal surface significantly decreases the non‐specific cellular uptake of the EXOs into the DCs. However, the EXOs with mannose‐conjugated PEG‐DSPE (EXO‐PEG‐man) exhibit excellent intracellular uptake into the DCs and boost the immune response by the incorporation of adjuvant, monophosphoryl lipid A (MPLA) within the EXO. After an intradermal injection, a higher retention of EXO‐PEG‐man is observed in the lymph nodes, which could be used for the efficient delivery of immune stimulators and antigens to the lymph nodes in vivo.
Assessment of the association between ACYP2 and laryngeal squamous cell carcinoma risk in Chinese males
Authors: Zhao Wenhui , Niu Fanglin , Xie Zhilan , Yan Mengdan , Li Jingjie , Zhang Yuan , Chen Jun , Liu Qiufang , Jin Tianbo 
Published: May 2019

AbstractBackgroundLaryngeal squamous cell carcinoma (LSCC) is one of the most prevalent malignant neoplasms of the upper respiratory tract. Studies have confirmed that an unstable chromosome constitution promotes the progress of laryngeal tumorigenesis, and ACYP2 has been confirmed as a telomere length‐related gene. However, to date, the association between ACYP2 polymorphisms and LSCC susceptibility has not been investigated.MethodsWe performed this study to explore the effect of 11 single‐nucleotide polymorphisms (SNPs) in ACYP2 on LSCC susceptibility in Chinese Han males. Unconditional logistic regression analysis adjusted for age was used to calculate the odds ratios and 95% confidence intervals.ResultsBased on allele and genotype models, our results showed that rs1682111 variant was significantly associated with a decreased LSCC susceptibility (p < 0.05). On the contrary, polymorphisms of rs10439478, rs11125529, rs12615793, rs843711, rs11896604, and rs17045754 were significantly associated with an increased LSCC risk (p < 0.05). The results of haplotype analysis indicated that haplotypes “TTCTCG” and “TTCTAA” in block 1 and “TG” in block 2 showed a risk factor for the development of LCSS (p = 0.009, p < 0.001, and p = 0.001, respectively). The results of Genotype‐Tissue Expression analysis indicate that these significant SNPs were known to be associated with ACYP2 expression.ConclusionOur data demonstrated that ACYP2 polymorphisms may exert effects on LSCC susceptibility in Chinese Han males.
Oral Immunotherapy with Egg Peptides Induces Innate and Adaptive Tolerogenic Responses
Authors: Lozano‐Ojalvo Daniel , Martínez‐Blanco Mónica , Pérez‐Rodríguez Leticia , Molina Elena , López‐Fandiño Rosina 
Published: May 2019

ScopeThe mechanism through which peptide‐based immunotherapy provides effective desensitization toward food allergy is investigated.Methods and resultsEx vivo experiments are conducted with intestinal epithelial cells (IECs), dendritic cells (DCs), and T cells from mice sensitized to egg white (EW) and either left untreated or tolerized by the oral administration of a hydrolysate of ovalbumin with pepsin (OP). IECs from EW‐sensitized mice upregulate Il33 and Tslp to a higher extent than those from tolerized mice and induce bone marrow (BM)‐DCs to express Tnfsf4 and produce pro‐inflammatory cytokines. On the other hand, incubation with OP upregulates Aldh1a1 in IEC cultures and BM‐DCs conditioned with supernatants of OP‐pulsed IECs also overexpress Aldh1a2 and Tgfb1. DCs from tolerized mice, in co‐culture with CD4+ T cells from sensitized mice, reduce the secretion of IL‐5, IFN‐γ, and IL‐17, following stimulation with EW, to a level similar than DCs from sham‐sensitized mice. Furthermore, incubation with OP of DCs and CD4+ T cells, regardless of the mouse sentitization status, promotes the secretion of TGF‐β and the generation of Foxp3+ RORγt+ cells.ConclusionOP induces the expression of aldehyde dehydrogenase enzymes in cells of the innate immune system and the development of Foxp3+ RORγt+ T cells.
Current tobacco use, nicotine dependence, and transitions across stages of alcohol involvement: A latent transition analysis approach
Authors: Pacek Lauren R. , Reboussin Beth A. , Green Kerry M. , LaFlair Lareina N. , Storr Carla L. , Alvanzo Anika A.H. , Mojtabai Ramin , Cullen Bernadette , Young Andrea S. , Tormohen Kayla , Riehm Kira , Crum Rosa M. 
Published: May 2019

AbstractObjectivesThis study aims to examine the (a) probability of transition between stages of alcohol involvement and (b) influence of tobacco use and nicotine dependence on transitions.MethodsData came from Waves 1 and 2 of the National Epidemiologic Survey on Alcohol and Related Conditions. Latent transition analysis estimated the probability of transitioning between stages of alcohol involvement across waves and the impact of tobacco use and nicotine dependence at Wave 1 on transitions.ResultsMales reporting current tobacco use but no dependence at Wave 1 were more likely to progress from No Problems to Moderate Problems (adjusted odds ratio [aOR] = 1.79; 95% confidence interval [CI] [1.44, 2.22]) and from No Problems to Severe Problems (aOR = 2.44; 95% CI [1.25, 4.77]) than nontobacco users. Females reporting current tobacco use but no dependence were more likely to progress from No Problems to Moderate Problems (aOR = 2.00; 95% CI [1.37, 2.94]) and from No Problems to Severe Problems (aOR = 2.87; 95% CI [1.34, 6.13]). Females reporting current tobacco use and dependence were more likely than females not using tobacco to transition from Moderate to No Problems (aOR = 2.10; 95% CI [1.04, 4.22]).ConclusionsResults suggest that tobacco use is a preceding correlate of progression in alcohol involvement among males and females. Among females, tobacco use and nicotine dependence are also related to alcohol involvement recovery.