bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the Environmental Parameters of Hydroponic Crop Grown under Polyhouse Conditions in Different Seasons
Authors: Rani R. Sudha , Kumar H. V. Hema , Mani A. , Reddy Boreddy Sreenivasula , Rao Ch. Sujani 
Published: November 2024

A study was conducted on Basil (Ocimum Basilicum) crop grown under Polyhouse hydroponics in different seasons by controlling the environmental parameters at Dr. NTR College of Agricultural Engineering, Bapatla. The research work conducted for four seasons i.e., Season1(April and May), Season2(August and September), Season3(October and November) and Season4(January to February) on environmental parameters is presented in this paper. The environmental parameters were tried to be controlled to maintain a proper relative humidity range, i.e., 60-75%, a temperature below 30 °C, and CO2 levels of 600 to 1500 ppm. The controlled environment and observations recorded by using Rotronic CP11’for crop analysis purpose. This makes a good quality and disease-free crop production. The final results reveal that the highest yield of 41.7 kg in seaso, followed by 40.5 kg in season2, 38.9 kg in season3, and 36.2 kg in season4. The lowest yield of all the seasons was 36.2 kg in season4. Ultimately, the experiment showed that the basil crop grows in good health throughout Season1 and then again during Season2. Polynomial equations were fitted for the humidity and temperatures maintained inside the hydroponic polyhouse which are discussed in this paper. R2 values between 0.5 and 0.7 were obtained for all the fitted equations. According to the study, yield has a positively correlated with temperature and negatively correlated with relative humidity and carbon dioxide.
Tolerance of Phosphorus and Potassium Solubilizing Bacteria to Fungicides In vitro
Authors: Patil Neha Hemant , Magar Sunita J. , Somwanshi S. D. , Mali Rajashri Harishchandra , Nashte Gauri Ganesh 
Published: November 2024

Phosphorus solubilizing bacteria (PSB) and Potassium solubilizing bacteria (KSB) plays a vital role in converting these nutrients into forms that plants can readily absorb, thus enhancing soil fertility and crop yields. The compatibility of phosphorus and potassium solubilizing bacteria with 8 fungicides was tested under laboratory condition. The eight fungicides tested against PSB-1, the Carbendazim 50% WP, Propiconazole 25% EC and Carbendazim 12% + Mancozeb 63% WP were found highly compatible with phosphorus solubilizing bacteria and rest of the five fungicides viz., Mancozeb 75% WP, Zineb 75% WP, Metalaxyl 35 % WS, Azoxystrobin 18.2 % + Difenoconazole 11.4 % SC, Azoxystrobin 11% + Tebuconazole 18.3 % were incompatible with the test bacterium. The treatment T8 (Azoxystrobin 11% + Tebuconazole 18.3 %) were found statistically at par at 48 and 72 hrs of incubation at 50% of recommended dose and recommended dosages. the eight fungicides tested against KSB-2 Carbendazim 50% WP, Propiconazole 25% EC and Carbendazim 12% + Mancozeb 63% WP were found highly compatible with phosphorus solubilizing bacteria and rest of the five fungicides viz., Mancozeb 75% WP, Zineb 75% WP, Metalaxyl 35 % WS, Azoxystrobin 18.2 % + Difenoconazole 11.4 % SC, Azoxystrobin 11% + Tebuconazole 18.3 % were incompatible bacterium with the test bacterium. The treatment T8 (Azoxystrobin 11% + Tebuconazole 18.3% SC) and treatment T1 (Mancozeb 75% WP) were found statistically at par at 48 and 72 hrs of incubation at 50% of recommended dose and recommended dosages.
Estimating relationships between size and fecundity in the threatened giant garter snake in seminatural and agricultural wetlands
Authors: Scherer Rick D. , Hansen Eric C. , Joseph Max , Wack Raymund F. 
Published: April 2019

AbstractIncreasing numbers of populations of threatened and endangered plants and animals occur in environments altered by human activity. Life history traits may vary among populations in natural and human‐dominated environments, and therefore, management strategies may need to be tailored to different environments to promote persistence. The primary objectives of this project were to estimate the probability of being pregnant for females of the threatened giant garter snake (Thamnophis gigas) and test for differences in these demographic parameters between populations in seminatural wetlands and wetlands in areas dominated by rice agriculture. We also hypothesized that the probability of breeding and the number of fetuses would increase with increasing body size, a commonly reported relationship for snakes. We analysed data on T. gigas fecundity from 9 years using a Bayesian hurdle model and found no evidence of variation in the probability of being pregnant or in the number of fetuses given pregnancy for T. gigas in the two environments. The probability of being pregnant in a year was higher for females of larger size and ranged from <0.10 for snakes smaller than 631 mm snout‐to‐vent length (SVL) to >0.96 for the largest females. Similarly, given that a female was pregnant, the number of fetuses produced was higher for larger females. Pregnant females of average SVL were expected to produce 14 fetuses, and the largest females were expected to produce 34 fetuses. The inferences from our analyses are needed for subsequent population modeling, which will guide planning and decision‐making for T. gigas.
Evidence for weak interaction between phytochromes Agp1 and Agp2 from Agrobacterium fabrum
Authors: Xue Peng , El Kurdi Afaf , Kohler Anja , Ma Hongju , Kaeser Gero , Ali Arin , Fischer Reinhard , Krauß Norbert , Lamparter Tilman 
Published: April 2019

During bacterial conjugation, plasmid DNA is transferred from cell to cell. In Agrobacterium fabrum, conjugation is regulated by the phytochrome photoreceptors Agp1 and Agp2. Both contribute equally to this regulation. Agp1 and Agp2 are histidine kinases, but, for Agp2, we found no autophosphorylation activity. A clear autophosphorylation signal, however, was obtained with mutants in which the phosphoaccepting Asp of the C‐terminal response regulator domain is replaced. Thus, the Agp2 histidine kinase differs from the classical transphosphorylation pattern. We performed size exclusion, photoconversion, dark reversion, autophosphorylation, chromophore assembly kinetics and fluorescence resonance energy transfer measurements on mixed Agp1/Agp2 samples. These assays pointed to an interaction between both proteins. This could partially explain the coaction of both phytochromes in the cell.
Arrested Development of Henneguya ictaluri (Cnidaria: Myxobolidae) in ♀ Channel Catfish × ♂ Blue Catfish Hybrids
Authors: Rosser Thomas G. , Khoo Lester H. , Wise David J. , Mischke Charles C. , Greenway Terrence E. , Alberson Neely R. , Reichley Stephen R. , Woodyard Ethan T. , Steadman James , Ware Cynthia , Pote Linda M. , Griffin Matt J. 
Published: April 2019

Abstract
Henneguya ictaluri is the etiologic agent of proliferative gill disease (PGD) in farm-raised Channel Catfish Ictalurus punctatus and hybrid catfish in the southeastern United States, and significant annual losses are attributed to this disease. Research suggests that H. ictaluri infection dynamics in Blue Catfish I. furcatus and hybrid catfish (Channel Catfish × Blue Catfish) differ from those in Channel Catfish. Two separate infectivity trials were conducted to investigate H. ictaluri development in Channel Catfish, Blue Catfish, and their hybrids. On two separate occasions with two different year-classes, fish were exposed to pond water containing H. ictaluri actinospores and sampled weekly for 12 weeks (trial 1) or 14 weeks (trial 2). In trial 1, the presence of H. ictaluri was evaluated histologically and by quantitative PCR of fish tissues, including gills, blood, anterior kidney, brain, heart, liver, posterior kidney, spleen, and stomach. Henneguya ictaluri DNA was detected in significantly higher concentrations throughout multiple organ systems in the Channel Catfish compared to the hybrid catfish and Blue Catfish, with the gills having higher quantities. Myxospores were observed in Channel Catfish gill tissue at 8 weeks postexposure. No myxospores were observed in Blue Catfish or hybrid catfish. The second trial focused on gills only and yielded similar results, with Channel Catfish having significantly greater H. ictaluri DNA quantities than hybrids or Blue Catfish across all time points. Myxospores were observed in Channel Catfish beginning at 6 weeks postexposure and were found in 36% (58/162) of Channel Catfish sampled for molecular and histological analysis during weeks 6–14. Myxospores in hybrid catfish were sparse, with single pseudocysts observed in two hybrid catfish (1.2%) at 14 weeks postexposure. These results imply arrested development of H. ictaluri in hybrid catfish. As such, culture of hybrid catfish may be an effective management strategy to minimize the burden of PGD.
Glycometabolic Bioorthogonal Chemistry‐Guided Viral Transduction for Robust Human T Cell Engineering
Authors: Pan Hong , Li Ping , Li Guifei , Li Wenjun , Hu Bian , He Huamei , Chen Ze , Wang Fangfang , Liu Lanlan , Gong Yifeng , Han Yutong , Luo Yingmei , Zheng Mingbin , Ma Yifan , Cai Lintao , Jin Yan 
Published: April 2019

AbstractGenetically engineered T cell therapy is emerging as a potent strategy for treating hematological and solid malignancies. Although lentivirus is the most common vector for T cell gene modification, its transduction efficacy remains unsatisfied especially during the manufacturing process. Herein, glycometabolic bioorthogonal chemistry is utilized to establish a highly efficient viral transduction system for human primary T lymphocytes. Azide motifs are anchored on the T cell surface via the intrinsic glycometabolism of exogenous azide–glucose, serving as an artificial ligand for viral binding. The complementary functional moiety dibenzocyclooctyl (DBCO)‐conjugated PEI1.8K (PEI‐DBCO) is then coated on lentiviral surface, which strengthens the virus–T cell interaction through DBCO/azide bioorthogonal chemistry. The results show that the PEI‐DBCO/azide–glucose system effectively facilitates viral binding to T cells and elevates the transduction efficiency of the lentivirus from 20% to 80% without any effect on T cell proliferation and activity. More importantly, the PEI‐DBCO/azide–glucose system significantly doubles the yield of anti‐CD19 chimeric antigen receptor T (CAR‐T) cells and robustly boosts their antitumor capability compared to polybrene‐assisted lentiviral transduction both in vitro and in vivo. Overall, the bioorthogonal PEI‐DBCO/azide–glucose system significantly boosts viral transduction efficacy and exhibits a powerful gene‐manipulating capability in human primary T cells, thereby showing a great potential for clinical‐engineered T lymphocytes manufacture.
Micro Light‐Emitting Diodes for Display and Flexible Biomedical Applications
Authors: Lee Han Eol , Shin Jung Ho , Park Jung Hwan , Hong Seong Kwang , Park Sang Hyun , Lee Seung Hyung , Lee Jae Hee , Kang Il‐Suk , Lee Keon Jae 
Published: April 2019

AbstractInorganic‐based micro light‐emitting diodes (µLEDs) have witnessed significant improvements in terms of display and biomedical applications, which can shift the paradigm of future optoelectronic systems. In particular, µLED displays are on the verge of becoming the next big interface platform for visual communications, expanding to various internet of things and wearable/bioapplications. Novel µLED concepts need to be upgraded to be able to satisfy their potential optoelectric applications, such as virtual reality, smart watches, and medical sensors for individual computing in this hyperconnected society. Here, representative progresses in the field of flexible µLEDs are reviewed with regard to device structures, massive µLED transfers, methods for performance enhancement, and applications.
Nanomaterials in Proteomics
Authors: Sun Nianrong , Wu Hao , Shen Xizhong , Deng Chunhui 
Published: April 2019

AbstractFor a deep understanding of the human proteome, the first crucial step is always the effective separation of targeted proteins/peptides from complex samples. In mass spectrometry‐based proteomics, nanomaterials have emerged as a highly effective means to separate targeted proteins/peptides and increase their relative abundance, which is beneficial to conduct mass spectrometric analysis. In particular, nanomaterials with different specific functionalities have received great attention in ordinary proteomics, phosphoproteomics, and glycoproteomics, for which the easily recognizable characteristics of these proteomes take the primary responsibility, such as the hydrophobicity, phosphate groups, cis–diol structure, and hydrophilicity. This review mainly focuses on the recent design and preparation of nanomaterials with specific recognition ability, as well as their application in the aforementioned three types of proteomics.
Enhanced Neurite Outgrowth on a Multiblock Conductive Nerve Scaffold with Self‐Powered Electrical Stimulation
Authors: Sun Yi , Quan Qi , Meng Haoye , Zheng Yudong , Peng Jiang , Hu Yaxin , Feng Zhaoxuan , Sang Xiao , Qiao Kun , He Wei , Chi Xiaoqi , Zhao Liang 
Published: April 2019

AbstractElectrical stimulation (ES) is widely applied to promote nerve regeneration. Currently, metal needles are used to exert external ES, which may cause pain and risk of infection. In this work, a multiblock conductive nerve scaffold with self‐powered ES by the consumption of glucose and oxygen is prepared. The conductive substrate is prepared by in situ polymerization of polypyrrole (PPy) on the nanofibers of bacterial cellulose (BC). Platinum nanoparticles are electrodeposited on the anode side for glucose oxidation, while nitrogen‐doped carbon nanotubes (N‐CNTs) are loaded on the cathode side for oxygen reduction. The scaffold shows good mechanical property, flexibility and conductivity. The scaffold can form a potential difference of above 300 mV between the anode and the cathode in PBS with 5 × 10−3mglucose. Dorsal root ganglions cultured on the Pt‐BC/PPy‐N‐CNTs scaffold are 55% longer in mean neurite length than those cultured on BC/PPy. In addition, in vivo study indicates that the Pt‐BC/PPy‐N‐CNTs scaffold promotes nerve regeneration compared with the BC/PPy group. This paper presents a novel design of a nerve scaffold with self‐powered ES. In the future, it can be combined with other features to promote nerve regeneration.
2D Nanoclay for Biomedical Applications: Regenerative Medicine, Therapeutic Delivery, and Additive Manufacturing
Authors: Gaharwar Akhilesh K. , Cross Lauren M. , Peak Charles W. , Gold Karli , Carrow James K. , Brokesh Anna , Singh Kanwar Abhay 
Published: April 2019

AbstractClay nanomaterials are an emerging class of 2D biomaterials of interest due to their atomically thin layered structure, charged characteristics, and well‐defined composition. Synthetic nanoclays are plate‐like polyions composed of simple or complex salts of silicic acids with a heterogeneous charge distribution and patchy interactions. Due to their biocompatible characteristics, unique shape, high surface‐to‐volume ratio, and charge, nanoclays are investigated for various biomedical applications. Here, a critical overview of the physical, chemical, and physiological interactions of nanoclay with biological moieties, including cells, proteins, and polymers, is provided. The state‐of‐the‐art biomedical applications of 2D nanoclay in regenerative medicine, therapeutic delivery, and additive manufacturing are reviewed. In addition, recent developments that are shaping this emerging field are discussed and promising new research directions for 2D nanoclay‐based biomaterials are identified.
Plasmonic‐Nanopore Biosensors for Superior Single‐Molecule Detection
Authors: Spitzberg Joshua D. , Zrehen Adam , van Kooten Xander F. , Meller Amit 
Published: April 2019

AbstractPlasmonic and nanopore sensors have separately received much attention for achieving single‐molecule precision. A plasmonic “hotspot” confines and enhances optical excitation at the nanometer length scale sufficient to optically detect surface–analyte interactions. A nanopore biosensor actively funnels and threads analytes through a molecular‐scale aperture, wherein they are interrogated by electrical or optical means. Recently, solid‐state plasmonic and nanopore structures have been integrated within monolithic devices that address fundamental challenges in each of the individual sensing methods and offer complimentary improvements in overall single‐molecule sensitivity, detection rates, dwell time and scalability. Here, the physical phenomena and sensing principles of plasmonic and nanopore sensing are summarized to highlight the novel complementarity in dovetailing these techniques for vastly improved single‐molecule sensing. A literature review of recent plasmonic nanopore devices is then presented to delineate methods for solid‐state fabrication of a range of hybrid device formats, evaluate the progress and challenges in the detection of unlabeled and labeled analyte, and assess the impact and utility of localized plasmonic heating. Finally, future directions and applications inspired by the present state of the art are discussed.
Enhanced Thermal Conductivity and Balanced Mechanical Performance of PP/BN Composites with 1 vol% Finely Dispersed MWCNTs Assisted by OBC
Authors: Zha Xiang‐Jun , Yang Jie , Pu Jun‐Hong , Feng Chang‐Ping , Bai Lu , Bao Rui‐Ying , Liu Zheng‐Ying , Yang Ming‐Bo , Yang Wei 
Published: April 2019

Abstract1 vol% multiwalled carbon nanotubes (MWCNTs) is finely dispersed in poly(propylene) (PP)/boron nitride (BN) composites with the assistance of commercial ethylene‐α‐octene block copolymer (OBC) through a conventional melt‐compounding method, whereas serious agglomeration of MWCNTs occurs in the melt compounded PP/MWCNTs/BN composites. The good dispersion of MWCNTs facilitates the formation of a better conductive network to synergistically improve the thermal conductivity of PP/OBC/MWCNT/BN composites, up to 1.71 W m−1 K−1 at a BN content of 20 vol%. Meanwhile, the elastomeric OBC component leads to markedly enhanced impact toughness (>8.0 kJ m−2) and elongation at break (>23%) of the composites. This work sheds light on the fabrication of thermally conductive polymer composites with balanced mechanical performance through a conventional processing technique.
High‐Performance Organic Field‐Effect Transistor with Matching Energy‐Band Alignment between Organic Semiconductor and the Charge‐Trapping Dielectric
Authors: Wang Yiru , Yang Youbin , Ding Ping , Wei Qi , Gao Xu , Wang Suidong , Liu Chang , Li Aidong , Yin Jiang , Xia Yidong , Liu Zhiguo 
Published: April 2019

AbstractHigh‐performance bottom‐gate nonvolatile organic field‐effect transistor (OFET) devices based on a special matching energy‐band alignment between the organic semiconductor pentacene and the charge‐trapping dielectric ZnTe are reported. The lower potential difference between the conduction band minimum of ZnTe and the lowest unoccupied molecular orbital of pentacene with a weak electron conductivity endows the OFET a memory window of 10 V at an applied sweeping gate‐voltage of ±15 V, a high ION/IOFF ratio of more than 106, and good retention with a high ION/IOFF ratio of 6 × 105 after 104 s. The large memory window of the OFET is attributed to the unique energy‐band alignment of the memory device and the high density of traps in Te‐deficient ZnTe film, and the prominent retention is attributed to the deeply trapped electrons in the potential well formed by Al2O3 tunneling and blocking layers.
Constitutive activation of the PI3K‐AKT pathway and cardiovascular abnormalities in an individual with Kosaki overgrowth syndrome
Authors: Zarate Yuri A. , Boccuto Luigi , Srikanth Sujata , Pauly Rini , Ocal Eylem , Balmakund Tonya , Hinkle Kevin , Stefans Vikki , Schaefer Gerald B. , Collins Ronnie Thomas 
Published: April 2019

AbstractKosaki overgrowth syndrome is a recently described syndrome characterized by distinctive facial features, brain white matter lesions, and developmental delay. Germline activating heterozygous PDGFRB mutations have been reported in this condition. Systemic connective tissue‐type findings have been described in some individuals. We describe a 19‐year‐old Caucasian female with a history of hydrocephalus, Dandy–Walker malformation, cervical spine arachnoid cyst, progressive scoliosis, and overgrowth. Her physical exam included distinctive craniofacial dysmorphism, as well as soft and hyperextensible skin. Cardiovascular imaging during adolescence revealed saccular aneurysms in both coronary artery systems and subtle tortuosity of the cervical vertebral arteries. Exome sequencing trio analysis identified a de novo previously reported pathogenic variant in PDGFRB, c.1696T>C (p.[Trp566Arg]). Further functional studies included platelet‐derived growth factor cellular metabolic pathway activity that confirmed the variant causes a constitutive activation of the PI3K‐AKT pathway. This is the first report to characterize the activating nature of this PDGFRB variant. We also highlight the connective tissue findings seen in Kosaki overgrowth syndrome and recommend baseline echocardiographic evaluation in all individuals with this condition with particular emphasis on coronary arteries.
Primate microbiomes over time: Longitudinal answers to standing questions in microbiome research
Authors: Björk Johannes R. , Dasari Mauna , Grieneisen Laura , Archie Elizabeth A. 
Published: April 2019

AbstractTo date, most insights into the processes shaping vertebrate gut microbiomes have emerged from studies with cross‐sectional designs. While this approach has been valuable, emerging time series analyses on vertebrate gut microbiomes show that gut microbial composition can change rapidly from 1 day to the next, with consequences for host physical functioning, health, and fitness. Hence, the next frontier of microbiome research will require longitudinal perspectives. Here we argue that primatologists, with their traditional focus on tracking the lives of individual animals and familiarity with longitudinal fecal sampling, are well positioned to conduct research at the forefront of gut microbiome dynamics. We begin by reviewing some of the most important ecological processes governing microbiome change over time, and briefly summarizing statistical challenges and approaches to microbiome time series analysis. We then introduce five questions of general interest to microbiome science where we think field‐based primate studies are especially well positioned to fill major gaps: (a) Do early life events shape gut microbiome composition in adulthood? (b) Do shifting social landscapes cause gut microbial change? (c) Are gut microbiome phenotypes heritable across variable environments? (d) Does the gut microbiome show signs of host aging? And (e) do gut microbiome composition and dynamics predict host health and fitness? For all of these questions, we highlight areas where primatologists are uniquely positioned to make substantial contributions. We review preliminary evidence, discuss possible study designs, and suggest future directions.