bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of Distinct Facets of Perfectionism and Sociocultural Idealization of Thinness on Disordered Eating Symptoms
Authors: Habashy Jessica , Culbert Kristen M. 
Published: April 2019

Introduction: Perfectionism is a multidimensional personality style implicated in risk for disordered eating (DE), yet the types of perfectionism most predictive of DE and how perfectionistic tendencies lead to the development of DE remains less clear. This study aimed to determine the types of perfectionism most strongly associated with DE and whether perceived pressures for thinness and thin-ideal internalization serve as intervening variables that explain associations between perfectionism and DE.Methods: An ethnically diverse sample of female college students (n = 154–292) completed well-validated self-report questionnaires assessing overall DE symptoms, perfectionism, and two sociocultural factors: pressures for thinness and thin-ideal internalization.Results: Only perfectionism constructs encompassed under the “maladaptive evaluative concerns” domain were significantly associated with DE. Double mediation models indicated that heightened pressures for thinness and thin-ideal internalization fully mediate perfectionism-DE associations.Discussion: Results suggest that disorder-specific risk factors related to idealization of thinness may be important for understanding how a transdiagnostic factor, like perfectionism, results in DE.
Genetic and Molecular Analysis of Essential Genes in Centromeric Heterochromatin of the Left Arm of Chromosome 3 inDrosophila melanogaster
Authors: Syrzycka Monika , Hallson Graham , Fitzpatrick Kathleen A , Kim Inho , Cotsworth Shawn , Hollebakken Rob E , Simonetto Kevin , Yang Linda , Luongo Stephanie , Beja Kevin , Coulthard Alistair B , Hilliker Arthur J , Sinclair Donald A , Honda Barry M 
Published: April 2019

AbstractA large portion of the Drosophila melanogaster genome is contained within heterochromatic regions of chromosomes, predominantly at centromeres and telomeres. The remaining euchromatic portions of the genome have been extensively characterized with respect to gene organization, function and regulation. However, it has been difficult to derive similar data for sequences within centromeric (centric) heterochromatin because these regions have not been as amenable to analysis by standard genetic and molecular tools. Here we present an updated genetic and molecular analysis of chromosome 3L centric heterochromatin (3L Het). We have generated and characterized a number of new, overlapping deficiencies (Dfs) which remove regions of 3L Het. These Dfs were critically important reagents in our subsequent genetic analysis for the isolation and characterization of lethal point mutations in the region. The assignment of these mutations to genetically-defined essential loci was followed by matching them to gene models derived from genome sequence data: this was done by using molecular mapping plus sequence analysis of mutant alleles, thereby aligning genetic and physical maps of the region. We also identified putative essential gene sequences in 3L Het by using RNA interference to target candidate gene sequences. We report that at least 25, or just under 2/3 of loci in 3L Het, are essential for viability and/or fertility. This work contributes to the functional annotation of centric heterochromatin in Drosophila, and the genetic and molecular tools generated should help to provide important insights into the organization and functions of gene sequences in 3L Het.
High-Efficiency CRISPR/Cas9-Mediated Gene Editing in Honeybee (Apis mellifera) Embryos
Authors: Hu Xiao Fen , Zhang Bo , Liao Chun Hua , Zeng Zhi Jiang 
Published: April 2019

Abstract
The honeybee (Apis mellifera) is an important insect pollinator of wild flowers and crops, playing critical roles in the global ecosystem. Additionally, the honeybee serves as an ideal social insect model. Therefore, functional studies on honeybee genes are of great interest. However, until now, effective gene manipulation methods have not been available in honeybees. Here, we reported an improved CRISPR/Cas9 gene-editing method by microinjecting sgRNA and Cas9 protein into the region of zygote formation within 2 hr after queen oviposition, which allows one-step generation of biallelic knockout mutants in honeybee with high efficiency. We first targeted the Mrjp1 gene. Two batches of honeybee embryos were collected and injected with Mrjp1 sgRNA and Cas9 protein at the ventral cephalic side and the dorsal posterior side of the embryos, respectively. The gene-editing rate at the ventral cephalic side was 93.3%, which was much higher than that (11.8%) of the dorsal-posterior-side injection. To validate the high efficiency of our honeybee gene-editing system, we targeted another gene, Pax6, and injected Pax6 sgRNA and Cas9 protein at the ventral cephalic side in the third batch. A 100% editing rate was obtained. Sanger sequencing of the TA clones showed that 73.3% (for Mrjp1) and 76.9% (for Pax6) of the edited current-generation embryos were biallelic knockout mutants. These results suggest that the CRISPR/Cas9 method we established permits one-step biallelic knockout of target genes in honeybee embryos, thereby demonstrating an efficient application to functional studies of honeybee genes. It also provides a useful reference to gene editing in other insects with elongated eggs.
Karyotype of native Zea mays ssp. mays Proto-Confite morocho
Authors: López-Medina Segundo Eloy , Mostacero León José , Quijano-Jara Carlos Heli , Gil-Rivero Armando Efraín , Rabanal-Che-León Maria Fernanda 
Published: April 2019

Corn is a crop of great importance in the world since it constitutes a basic component of the diet of the population; a production of 1026.61 million tons was estimated during the present year. Currently, there are many theories about the wild ancestors of corn, being Proto-Confite Morocho one of the primitive races in Peru, which is attributedas the ancestor of numerous native varieties. Therefore, knowledge of its genetic constitution is important since it gives us the possibility of finding phylogenetic affinities and indicators of systematic classification. The present study was carried out with the purpose of elaborating the karyotype of Zea mays ssp. mays ‘native corn’ Proto-Confite morocho and determine the number and morphology of the chromosomes. Corn seeds from the province of Virú, La Libertad, Peru, were used, while the experimental phase was developed in the Laboratory of Geneticsand Molecular Biology at the National University of Trujillo, where metaphase plaques were obtained, selecting the best to perform the karyotype. It was concluded that the Zea mays ssp. mays ‘native corn’ Proto-Confite morocho presents 3 pairs of chromosomes ( 4, 4’, 8, 8’, 10, 10’) submetacentric and 7 pairs of chromosomes (1, 1’, 2, 2’,3, 3’, 5, 5’, 6, 6’, 7, 7’, 9, 9’) metacentric, which average size are between 4.16 and 1.88 μm.
A Tellegen Abszorpciós Skála részletes pszichometriai elemzése
Authors: Rózsa Sándor , Hupuczi Ernő , Martin László , Birkás Béla , Hartung István , Hargitai Rita , Varga József , Láng András , Tiringer István , Kállai János 
Published: April 2019

Háttér
Az abszorpció mérésére a leggyakrabban a 34 tételes Tellegen Abszorpció Skálátalkalmazzák. Az önjellemző mérőeszköz széles körű elterjedtségének ellenére viszonylag‍csekély azon közlemények száma, amelyek a mérőeszköz részletes pszichometriai jellemzőinekfeltárására (pl. faktorszerkezet, skálák belső konzisztenciája) irányulnak.

Célkitűzés
Vizsgálatunk fő kérdésfeltevése az, hogy az abszorpció mennyire tekinthető önálló, látensdimenziónak, bontható-e további alfaktorokra, illetve az alfaktorokból képzett skálák milyen‍megbízhatóságúak, és mennyivel árnyalják a főskálán szerzett eredményeket. Kíváncsiakvoltunk arra is, hogy az 5-fokozatú Likert-skálás és a dichotóm válaszformátumok‍mennyire befolyásolják a mérőeszköz strukturális validitását és skáláinak belső homogenitását.

Módszerek
Az 5-fokozatú Likert-skálás válaszformátumú TAS-t összesen 1935hallgató töltötte ki, a dichotóm válaszformátumút pedig 399 személy. A mérőeszköz‍strukturális validitását feltáró és megerősítő faktorelemzések (pl. egyfaktoros modell, modellmásodrendű faktorral, bifaktoros modell), valamint parallel-elemzés segítségével‍vizsgáltuk. A kapott alksálák reliabilitását nemcsak a hagyományos módon (Cronbachalfa),hanem az általános faktor kontrollálása mellett is kiszámoltuk (hierarchikus omega,‍magyarázott közös variancia).

Eredmények
Az eredeti 34 tételes változatok (Likert-skálásés dichotóm válaszformátumú), illetve a feltáró faktorelemzések eredményei alapján rövidített‍23 tételes változat megerősítő faktorelemzései során egyértelműen a bifaktoroselrendezések adták a legjobb illeszkedési mutatókat és az általános faktor (főskála) megbízhatósága‍kiváló volt. Ezzel szemben az alkálák reliabilitása, az általános faktor kontrollálásamellett, már nem volt megfelelő. Az alkalmazható faktorok számának meghatározására‍a parallel-elemzés kiváló becslést adott, mind az 5-fokozatú Likert-skálás, mind adichotóm válaszformátum esetében az egydimenziós szerkezetet valószínűsítette.

Következtetések
A kapott eredményeink egyrészről alátámasztják azt, hogy az abszorpció egy látens pszichológiai változónak tekinthető, másrészről pedig a hagyományos statisztikaimódszerek korlátaira hívják fel a figyelmet, és a többféle módszer kombinált alkalmazásának‍hatékonyságát támasztják alá.
On the integrability problem for systems of partial differential equations in one unknown function, I
Authors: Kumpera Antonio 
Published: April 2019

We discuss the integration problem for systems of partial differential equations in one unknown function and special attention is given to the first order systems. The Grassmannian contact structures are the basic setting for our discussion and the major part of our considerations inquires on the nature of the Cauchy characteristics in view of obtaining the necessary criteria that assure the existence of solutions. In all the practical applications of partial differential equations, what is mostly needed and what in fact is hardest to obtains are the solutions of the system or, occasionally, some specific solutions. This work is based on four most enlightening Mémoires written by Élie Cartan in the beginning of the last century.
Automorphisms of Kronrod-Reeb graphs of Morse functions on 2-sphere
Authors: Kravchenko Anna , Maksymenko Sergiy 
Published: April 2019

Let $M$ be a compact two-dimensional manifold and, $f \in C^{\infty}(M, R)$ be a Morse function, and $\Gamma$ be its Kronrod-Reeb graph.Denote by $O(f)={f o h | h \in D(M)}$ the orbit of $f$ with respect to the natural right action of the group of diffeomorphisms $D(M)$ onC^{\infty}$, and by $S(f)={h\in D(M) | f o h = f }$ the coresponding stabilizer of this function.It is easy to show that each $h\in S(f)$ induces an automorphism of the graph $\Gamma$.Let $D_{id}(M)$ be the identity path component of $D(M)$, $S'(f) = S(f) \cap D_{id}(M)$ be the subgroup of $D_{id}(M)$ consisting of diffeomorphisms preserving $f$ and isotopic to identity map, and $G$ be the group of automorphisms of the Kronrod-Reeb graph induced by diffeomorphisms belonging to $S'(f)$. This group is one of key ingredients for calculating the homotopy type of the orbit $O(f)$.
In the previous article the authors described the structure of groups $G$ for Morse functions on all orientable surfacesdistinct from $2$-torus and $2$-sphere.
The present paper is devoted to the case $M = S^2$. In this situation $\Gamma$ is always a tree, and therefore all elements of the group $G$ have a common fixed subtree $Fix(G)$, which may even consist of a unique vertex. Our main result calculates the groups $G$ for all Morse functions $f: S^2 \to R$ whose fixed subtree $Fix(G)$ consists of more than one point.
Prognostic Nomogram for Overall Survival in Patients with Diffuse Large B-Cell Lymphoma
Authors: Han Ying , Yang Jianliang , Liu Peng , He Xiaohui , Zhang Changgong , Zhou Shengyu , Zhou Liqiang , Qin Yan , Song Yongwen , Sun Yan , Shi Yuankai 
Published: April 2019

Abstract

Purpose
This study aimed to develop a prognostic nomogram in diffuse large B-cell lymphoma (DLBCL) and compare it with traditional prognostic systems.

Materials and methods
We included 1,070 consecutive and nonselected patients with DLBCL in the National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences, between 2006 and 2012. A nomogram based on the Cox proportional hazards model was developed.

Results
The entire group were divided into the primary (n = 748) and validation (n = 322) cohorts. The 5-year overall survival (OS) rate was 64.1% for the entire group. Based on a multivariate analysis of the primary cohort, seven independent prognostic factors including age, Ann Arbor stage, Eastern Cooperative Oncology Group performance status score, lactate dehydrogenase, β2-microglobulin, CD5 expression, and Ki-67 index were identified and entered the nomogram. The calibration curve showed the optimal agreement between nomogram prediction and actual observation. In addition, the concordance index (C-index) of the nomogram for OS prediction was 0.77 (95% confidence interval [CI], 0.73–0.81) in the primary cohort and 0.76 (95% CI, 0.70–0.81) in the validation, superior to that of the international prognostic index (IPI), revised IPI (R-IPI), and National Comprehensive Cancer Network (NCCN)-IPI (range, 0.69–0.74, p&lt;.0001). Moreover, in patients receiving rituximab plus CHOP (R-CHOP) or R-CHOP-like regimens, compared with IPI (C-index, 0.73; 95% CI, 0.69–0.77), R-IPI (C-index, 0.70; 95% CI, 0.66–0.74), or NCCN-IPI (C-index, 0.71; 95% CI, 0.66–0.75), the DLBCL-specific nomogram showed a better discrimination capability (p &lt; .0001).

Conclusions
The proposed nomogram provided an accurate estimate of survival of patients with DLBCL, especially for those receiving R-CHOP or R-CHOP-like regimens, allowing clinicians to optimized treatment plan based on individualized risk prediction.

Implications for Practice
A diffuse large B-cell lymphoma (DLBCL)-specific prognostic nomogram was developed based on Chinese patients with DLBCL. As a tertiary hospital, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences is the number 1 ranked cancer center in China, with more than 800,000 outpatients in 2018. Patients included in this study were nonselected and came from 29 different provinces, municipalities, and autonomous regions in China. Thus, the data is believed to be representative to an extent.
Pantoprazole Affecting Docetaxel Resistance Pathways via Autophagy (PANDORA): Phase II Trial of High Dose Pantoprazole (Autophagy Inhibitor) with Docetaxel in Metastatic Castration-Resistant Prostate Cancer (mCRPC)
Authors: Hansen Aaron R. , Tannock Ian F. , Templeton Arnoud , Chen Eric , Evans Andrew , Knox Jennifer , Prawira Amy , Sridhar Srikala S. , Tan Susie , Vera-Badillo Francisco , Wang Lisa , Wouters Bradly G. , Joshua Anthony M. 
Published: April 2019

AbstractBackgroundEnhancing the effectiveness of docetaxel for men with metastatic castration-resistant prostate cancer (mCRPC) is an unmet clinical need. Preclinical studies demonstrated that high-dose pantoprazole can prevent or delay resistance to docetaxel via the inhibition of autophagy in several solid tumor xenografts.Materials and MethodsMen with chemotherapy-naive mCRPC with a prostate-specific antigen (PSA) &gt;10 ng/mL were eligible for enrolment. Men received intravenous pantoprazole (240 mg) prior to docetaxel (75 mg/m2) every 21 days, with continuous prednisone 5 mg twice daily. Primary endpoint was a confirmed ≥50% decline of PSA. The trial used a Simon's two-stage design.ResultsBetween November 2012 and March 2015, 21 men with a median age of 70 years (range, 58–81) were treated (median, 6 cycles; range, 2–11). Men had received prior systemic therapies (median, 1; range, 0–3), and 14 had received abiraterone and/or enzalutamide. PSA response rate was 52% (11/21), which did not meet the prespecified criterion (≥13/21 responders) to proceed to stage 2 of the study. At interim analysis with a median follow-up of 17 months, 18 (86%) men were deceased (15 castration-resistant prostate cancer, 2 unknown, 1 radiation complication). Of the men with RECIST measurable disease, the radiographic partial response rate was 31% (4/13). The estimated median overall survival was 15.7 months (95% confidence interval [CI], 9.3–19.6) and median PFS was 5.3 months (95% CI, 2.6–12.9). There were no toxic deaths, and all adverse events were attributed to docetaxel.ConclusionThe combination of docetaxel and pantoprazole was tolerable, but the resultant clinical activity was not sufficient to meet the ambitious predefined target to warrant further testing.Implications for PracticeTo date, no docetaxel combination regimen has reported superior efficacy over docetaxel alone in men with metastatic castration-resistant prostate cancer (mCRPC). The PANDORA trial has demonstrated that the combination of high dose pantoprazole with docetaxel is tolerable, but the clinical activity was not sufficient to warrant further testing. The chemotherapy standard of care for men with mCRPC remains docetaxel with prednisone. Future studies of autophagy inhibitors will need to measure autophagy inhibition accurately and determine the degree of autophagy inhibition required to produce a meaningful clinical response.
Complete Blood Count Score Model Integrating Reduced Lymphocyte-Monocyte Ratio, Elevated Neutrophil-Lymphocyte Ratio, and Elevated Platelet-Lymphocyte Ratio Predicts Inferior Clinical Outcomes in Adult T-Lymphoblastic Lymphoma
Authors: Feng Xiaoyan , Li Ling , Wu Jingjing , Zhang Lei , Sun Zhenchang , Li Xin , Wang Xinhua , Yu Hui , Chang Yu , Wu Xiaolong , Zhou Zhiyuan , Wang Guannan , Li Wencai , Li Zhaoming , Zhang Xudong , Zhang Mingzhi 
Published: April 2019

Abstract

Background
T-lymphoblastic lymphoma (T-LBL) is a highly aggressive neoplasm of lymphoblasts of T-cell origin. Although promising improvements have been recently achieved, one third of patients experience relapse or refractory T-LBL. Therefore, optimal strategies for identifying high-risk patients are urgently needed.

Materials and Methods
In the present study, 75 newly diagnosed adult patients (aged ≥15 years) with T-LBL were identified and the predictive value of complete blood count (CBC) abnormalities, including lymphocyte-monocyte ratio (LMR), neutrophil-lymphocyte ratio (NLR), and platelet-lymphocyte ratio (PLR) on clinical outcomes, was analyzed.

Results
Using the receiver operating characteristic curve to determine the best cutoff values based on survival, it was found that patients with T-LBL with LMR ≤2.8, NLR ≥3.3, and PLR ≥200 had both inferior progression-free survival (PFS) and inferior overall survival (OS), in which the differences were much more remarkable in the international prognostic index score 0–2 subgroup. In the multivariable analysis, NLR ≥3.3 together with age &gt;40 years and central nervous system (CNS) involvement were identified to be independently associated with shortened PFS, whereas PLR ≥200 and CNS involvement were identified to be independent risk factors for OS. LMR, NLR, and PLR were integrated to generate a “CBC score” model, which well separated adult patients with T-LBL into three risk groups, and the 3-year OS was 84%, 53%, and 30% for low-, intermediate-, and high-risk patients, respectively.

Conclusion
Overall, a “CBC score” model was initially promoted for stratification in adult patients with T-LBL using simple, widely available, and easy to interpret parameters in the largest adult T-LBL cohort to date.

Implications for Practice
Optimal strategies for identifying high-risk patients with T-lymphoblastic lymphoma (T-LBL) are urgently needed. In the largest adult T-LBL cohort to date, simple, inexpensive, widely available parameters were applied and revealed that patients with lymphocyte-monocyte ratio (LMR) ≤2.8, neutrophil-lymphocyte ratio (NLR) ≥3.3, and platelet-lymphocyte ratio (PLR) ≥200 had both inferior progression-free survival and inferior overall survival (OS), in which the differences were much more remarkable in the international prognostic index score 0–2 subgroup. LMR, NLR, and PLR were integrated to generate a “complete blood count score” model, in which the 3-year OS was 84%, 53%, and 30% for low-, intermediate-, and high-risk patients, respectively.
Effect of granular piles with geotextile encasement on strength tests of expansive clays
Authors: Phanikumar B. R. , Etukuri Raju Ramanjaneya 
Published: April 2019

This paper presents experimental data on unconfined compressive strength (UCS) and California bearing ratio (CBR) tests of expansive clay reinforced with granular piles (GPs) with and without geotextile encasement. One set of test samples were compacted at optimum moisture content and maximum dry density and were centrally reinforced with GPs of varying diameters and lengths. In another series of tests, the GP reinforcement was encased with a tensile geotextile. Both UCS and CBR test results increased with increasing length and diameter of the GPs. The test values further increased when the GPs were provided with geotextile encasement.
Multiplicação da planta medicinal Brossimum gaudichaudii Trécul (Moraceae) em meio de cultura
Authors: Carneiro Maurízia Fátima , Duarte Edson Ferreira , Nogueira João Carlos Mohn , Vargas Laureano Magno , Sibov Sergio Tadeu , Conceição Edemilson Cardoso 
Published: April 2019

Este trabalho teve como objetivo aprimorar técnicas de micropropagação para a produção in vitro com sementes e segmentos nodais de mama-cadela, planta importante no tratamento do vitiligo. Plant Preservative Mixture (PPM) e Hipoclorito de Sódio, em diferentes concentrações, foram usados para desinfestação de segmentos nodais e sementes. Sementes com tegumento, sem tegumento e eixo epicótilo-radícula foram testadas em meio 1/2 Murashige e Skoog (MS) para determinar a germinação. E para a multiplicação e enraizamento foram usados segmentos nodais axênicos jovens e, ou lignificados, em meio 1/2 MS contendo diferentes concentrações de citocininas e auxinas. Constatou-se que o PPM a 4,0 e 5,0 mL.L-1 proporcionou os menores graus de contaminação, contudo o hipoclorito não se mostrou efetivo. As sementes sem tegumento apresentaram 100% de germinação, IVG 2,0 e maior número de brotos. Plântulas jovens cultivadas em meio 1/2 MS com 1,0 mg.L-1 BAP produziram maior número de brotos, mas estes não apresentaram um crescimento adequado. O melhor crescimento de brotos e raízes foi obtido quando se usou segmentos nodais lignificados em meio 1/2 MS com BAP, 1,0 mg.L-1 e 2,0 mg.L-1 de AIA. O maior potencial de multiplicação ocorre para sementes sem tegumento e com segmentos nodais lignificados.