bluuebunny/crossref_metadata_2025_split huggingface dataset:
RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF RITONAVIR, OMBITASVIR AND PARITAPREVIR IN TABLET DOSAGE FORMS AND THEIR STRESS DEGRADATION STUDIES
Authors: BAJE SYED IBRAHIM , JYOTHI B. , MADHAVI N. 
Published: March 2019

Objective: The objective of the present study was to develop and validate a novel reverse phase high performance liquid chromatographic (RP-HPLC) method, for simultaneous determination of ritonavir (RIT), ombitasvir (OMB) and paritaprevir (PAR) in bulk mixtures, and in tablets.
Methods: Determination of the drugs ritonavir (RIT), ombitasvir (OMB), and paritaprevir (PAR), was carried out applying Hypersil BDS C18 column (250 mm X 4.6 mm i.e., 5 µm particle size), with photodiode array detector at λmax of 254 nm. The mobile phase applied for the current study composed of two solvents, i.e. A (0.01N % w/v potassium di-hydrogen orthophosphate buffer, pH 3.0 adjusted with dilute orthophosphoric acid) and B (acetonitrile). The mobile phase was pumped at a flow rate of 1.0 ml/min in the isocratic mode. The validation study with respect to specificity, linearity, precision, accuracy, and robustness, limit of detection (LOD) and limit of quantification (LOQ) was carried out employing the ICH guidelines.
Results: Ritonavir, ombitasvir, and paritaprevir showed linearity of response between 12.5-75 μg/ml for ritonavir, 3.125-18.75 µg/ml for ombitasvir and 18.75–112.5 µg/ml for paritaprevir, with a correlation coefficient (R2) 0.999, 0.999,0.999 for RIT, OMB, and PAR respectively. The % recovery obtained was 99.82±0.14 % RIT, OMB 100.03±0.96 % and for 99.96±0.26 % PAR. The LOD and LOQ values for RIT, OMB, PAR were obtained to be 0.02, 0.019and0.02, µg/ml and 0.07, 0.06 and 0.07 µg/ml, respectively. The method also exhibits good robustness for different chromatographic conditions like wavelength, flow rate, mobile phase, and injection volume.
Conclusion: The method was successfully employed, for the quantification of RIT, OMB, and PAR, in the quality control of in-house developed tablets, and can be applied for the industrial use.
FORMULATION AND EVALUATION OF AN ANTIMICROBIAL MUCOADHESIVE DENTAL GEL OF AZADIRACHTA INDICA AND GLYCYRRHIZA GLABRA
Authors: JAIN HEMANT K. , SWAMI PRERANA N. , GUJAR K. N. 
Published: March 2019

Objective: Objective of this study was to formulate and evaluate an antimicrobial mucoadhesive dental gel of herbal drugs for the prevention and treatment of dental plaque, dental caries, and periodontitis.
Methods: Azadirachta indica leaves extract was prepared with ethanol: water (80:20 v/v) and Glycyrrhiza glabra roots extract was prepared with ethanol: water (30:70 v/v). Both the extracts were evaluated for organoleptic properties, pH, phytochemical screening and total phenolic content. Thin layer chromatography (TLC) and Reverse-phase high-performance liquid chromatography (RP-HPLC) studies were performed. Antibacterial activity of the extract was done on Mueller Hinton agar media against Streptococcus mutans using the disc diffusion method. A mucoadhesive gel was prepared using carbopol 934, polyethylene glycol (PEG) 400 as a bioadhesive polymer and penetration enhancer, respectively. Three gel formulations were prepared at various concentrations of carbopol 934. Dental gel formulations were evaluated for different parameters such as appearance, pH, viscosity, spreadabilty, syringeability. Optimised batch was used for further studies viz. stability study, drug content, diffusion study to determine percent cumulative release of drug from gel formulation and in vitro mucoadhesion study.
Results: Rf value of nimbin and glycyrrhizin in TLC study, were found to be 0.08 and 0.56, respectively. RP-HPLC method was developed for estimation of active constituents present in both extracts using mobile phase acetonitrile: water [containing 0.1 % v/v glacial Acetic acid (GAA)]: methanol in the ratio of 30:60:10 at the flow rate of 0.8 ml/min. Rt of active constituents from both drug extracts was found to be 3.119 and 4.15 min, respectively. 2.5 % w/v of Azadirachta indica leaves extract showed a good zone of inhibition (10.66±0.577 mm) near to chlorhexidine (11.33±0.5773 mm). 1 % of Glycyrrhiza glabra roots extract exhibit antibacterial activity (9±1 mm) and masks the bitter taste of formulation. Batch (F2) was selected on the basis of viscosity, spreadabilty and syringeability. The optimised batch was found to be stable and has 83 % and 80.3 % of drug content. Percent cumulative releases of the drug from gel formulation during the diffusion study were found to be 87.52 % and 85.43 %. Adhesive force and adhesiveness were found to be 11.90 g and 0.92 millijoule, respectively during mucoadhesion study.
Conclusion: The evaluation parameters of optimized batch indicate the prepared dental gel is mucoadhesive, stable, good delivery drug system containing antimicrobial agents for prevention of plaque formation, dental caries and periodontitis.
ENHANCEMENT OF DISSOLUTION CHARACTERISTICS OF CLOPIDOGREL BISULPHATE BY PRONIOSOMES
Authors: MAZYED EMAN A. , ZAKARIA SHERIN 
Published: March 2019

Objective: The present investigation aims to formulate and evaluate proniosomes of clopidogrel bisulphate for improving its dissolution characteristics.
Methods: The slurry method was used for the preparation of proniosomes of clopidogrel using cholesterol, sorbitan monostearate (Span 60) and maltodextrin as a carrier. Clopidogrel proniosomes were evaluated for their entrapment efficiency and in vitro drug release. The best formula (F1) that achieved maximum drug release was further evaluated by measurement of the angle of repose, morphological examination, determination of vesicle size, determination of zeta potential, Fourier transform infrared spectroscopy and differential thermal analysis. The in vivo behavior of the selected proniosomal formula (F1) was studied by measuring the antiplatelet activity in adult male mice.
Results: The entrapment efficiency of clopidogrel proniosomes was in the range of 83.04±1.99 to 90.14±0.30. % drug released from proniosomal formulations was in the range of 79.73±0.35 to 97.70±1.10 % within 4 h. Clopidogrel proniosomes significantly enhanced the in vitro release of clopidogrel compared with the plain drug that achieved 61.77±2.22 % drug release. F1 significantly (p ≤ 0.001) increased the bleeding time and bleeding volume and significantly (p ≤ 0.05) prolonged prothrombin time and decreased prothrombin activity and increased the international normalized ratio (INR) compared to plain clopidogrel.
Conclusion: The present investigation introduced proniosomes as a promising carrier for clopidogrel that could enhance its dissolution and pharmacological effect.
OPTIMIZATION AND IN VITRO EVALUATION OF THE RELEASE OF CLASS II DRUG FROM ITS NANOCUBOSOMAL DISPERSION
Authors: Al-sakini Sadeq Jaafar , Maraie Nidhal Khazaal 
Published: March 2019

Objective: This work involves investigation and evaluation of the factors that affect the preparation and the release of the model class II drug (erythromycin) to optimize the efficiency of its prepared nanocubosomal dispersion to give very fast initial burst effect within the first hour that can continue for further two hours.
Methods: The work involved preparation of ten formulas of cubosomal dispersion by emulsifying different concentrations of glyceryl monooleate (GMO) (lipid content)/surfactant mixtures which were nano-sized and characterized morphologically by Transmission electronic microscopic (TEM), zeta potential, particle size, polydispersity index (pdI), pH, entrapment efficiency, conductivity test, dilution test and in vitro drug release.
Results: The selected nanocubosomal formula (F1) showed pH (7.41), particle size (315.05 nm), pdI (0.194), zeta potential (-30.852), entrapment efficiency (91%) and gave a 70% drug release within the first hour of the in vitro test and continued until it gave 96.3% drug release with further 2 h.
Conclusion: this work succeeded in preparing optimized cubosomal dispersion for erythromycin using different GMO/poloxamer 407 percent. The optimum formula gave an immediate release of the model drug (erythromycin) and it was ready to be incorporated in any suitable dosage form to give fast onset of action.
FORMULATION DEVELOPMENT AND STATISTICAL OPTIMIZATION OF A BILAYER TABLET OF BOSENTAN MONOHYDRATE AND SILDENAFIL CITRATE IN MANAGEMENT OF PULMONARY ARTERIAL HYPERTENSION
Authors: DIGHE PEARL PIRES , TANK H. M. 
Published: March 2019

Objective: The current study aims at fabrication of an oral bilayer matrix tablet of bosentan monohydrate and sildenafil citrate; the optimisation of their in vitro release and characterization, thereby reducing the side effects associated with bosentan, reducing dosing frequency and increasing patient compliance in the management of pulmonary arterial hypertension.
Methods: Methocel K4M Premium DC2, a directly compressible HPMC grade was used as the sustained release polymer. Pregelatinised starch is used as a diluent and release modifier and sodium lauryl sulphate (SLS) as a solubiliser. The blends of both layers were prepared, evaluated for precompression characteristics and compressed by direct compression. The compressed bilayer tablets were evaluated for their hardness, weight variation, friability, content uniformity and swelling index. The principle objective was to assess the influence of the above variables on in vitro drug release of Bosentan using a 23 factorial design. Responses are measured as drug release at 2h (Q2), 6h (Q6) and 10h (Q10).
Results: HPMC and pregelatinized starch form a synergistic gel thereby controlling drug release of bosentan for a 12 hour period. Batch BS09 consisting of 40 mg HPMC, 30 mg Pregeletinized starch and 5 mg SLS showed adequate controlled release for a 12 h period. Immediate release layer of sildenafil citrate showed optimum drug release of 102.96% within 30 min.
Conclusion: Bilayer tablet of bosentan and sildenafil is an ideal combination for patients failing monotherapy in pulmonary arterial hypertension.
CHARACTERIZATION OF HYDROXYPROPYL CELLULOSE PRODUCED FROM α-CELLULOSE BETUNG BAMBOO (DENDROCALAMUS ASPER) AND IT’S APPLICATION IN TABLET FORMULATION
Authors: Suryadi Herman , . Harmita , Akbar Muhammad Herpi , Lestari Pingkan , Lestari Pingkan 
Published: March 2019

Objective: This study aimed to obtain the physicochemical properties of hydroxypropyl cellulose (HPC) powder from α-cellulose Betung bamboo and its characteristics in tablet formulation.
Methods: HPC was prepared by hydroxypropylation of α-cellulose using 25% (w/v) sodium hydroxide and 10 ml propylene oxide (based on 1 g α-cellulose) at 70 °C for 3 h. HPC of Betung bamboo (HPC BB) was characterized using fourier transform infrared (FTIR) spectrometry, particle size analyzer (PSA), x-ray diffraction (XRD), scanning electron microscope (SEM) and compared to HPC grade SL (HPC SL) as the reference. Then, HPC BB was used as a binder in tablet formulation by direct compression method and the resulted tablets were evaluated. The tablets evaluation including weight and size uniformity, hardness, friability and disintegration time.
Results: The results showed HPC BB powder was yellowish white, odorless and tasteless, pH 7.49, residue on ignition 0.68%, hydroxypropoxy groups content 54.75%, average particle size 37.39 μm, loss on drying 1.09%, and moisture content 3.34%. Flow properties of powder fulfilled the requirements based on literature. Infrared spectrum and diffraction pattern of HPC BB were relatively similar to HPC SL. The tablets have average weight 403.495 mg, diameter 12.16 mm, thickness 3.11 mm, hardness 4.11 KPa, friability 2.04% and disintegration time 24.88 s.
Conclusion: Based on the comparison of powder characteristics and tablets evaluation, HPC BB has a great potential in tablet formulation which showed similar characteristics to reference.
THE OPTIMIZATION OF HPLC FOR QUANTITATIVE ANALYSIS OF ACID ORANGE 7 AND SUDAN II IN COSMETIC PRODUCTS USING BOX BEHNKEN DESIGN
Authors: PURBA NOVALINA BR , ROHMAN ABDUL , MARTONO SUDIBYO 
Published: March 2019

Objective: The objective of this study was to optimize high-performance liquid chromatography (HPLC) method for the determination of acid orange 7 (AO7) and sudan II (SII) in blusher product based on response surface methodology using box behnken design (BBD) approach.
Methods: Some factors responsible for HPLC separation including column temperature, mobile phase composition, flow rate were optimized using BBD. The responses evaluated were peak area, retention time, and tailing factor. AO7 and SII in blusher product has different properties, therefore both analytes were analysed using C18 column (Thermo Synergy Gold 250 mm x 4.6 mm i.d.,5 µm) using Shimadzu LC 20AD chromatograph equipped with photo-diode array (PDA) detector at 300-650 nm. The mobile phase used was acetonitrile-water (1:1 v/v), and acetonitrile composition was optimized at 35-50% for separation AO7 (ACN1), and 80-90% for SII (ACN2), delivered at the flow rate of 0.9–1 ml/min, using column temperature at 30-40 °C.
Results: BBD showed that separation of AO7 was influenced by the concentration of ACN1, flow rate and column temperature. These factors affected retention time, peak area, and tailing factor with peak area was the most significant. Tailing factor was not significantly affected by each factor, and retention time was slightly effected. Otherwise, Sudan II was affected by all these factors except ACN1. The optimal condition obtained based BBD was ACN1 43%, ACN2 90%, the flow rate of 0.9 ml/min and a column temperature of 40 °C.
Conclusion: BBD can be used to get optimum condition for analysis of AO7 and SII in blusher product.
AN EFFECT OF CARDIOPROTECTIVE ACTIVITY IN VARIOUS MEDICINAL PLANTS–A REVIEW
Authors: R. VIJAYA NIRMALA , R. ABINAYA 
Published: March 2019

Siddha system of medicines is known for its herbal in origin. Herbal plays a major role in treating acute and chronic diseases. Cardiovascular diseases (CVDs) is the life-threatening disease that contributes a leading cause of death day by day across the world. A change in lifestyle modification is the one of the risks of CVDs. This review paper attempts to facilitate the cardioprotective activity by providing different types of medicinal plants and its botanical distribution, description, chemical constituents and pharmacological activity. Few important herbs will protect the human being from CVDs and gives health and quality of life. In this paper, the authors explores the effect of cardioprotective activity in medicinal plants as follows, Terminalia chebula (Kadukkai), Piper longum (Thippili), Zingiber officinale (Inji), Tinospora cordifolia (Seenthil), Asparagus racemosus (Thanneervitan), Embelia ribes
(Vaaivilangam), Andrographis paniculata (Nilavembu), Nelumbo nucifera (Thaamarai), Allium sativum (Vellaipoondu), Bauhinia variegata (Mantharai).
Senectute, potestas, auctoritas...
Authors: Filippi María Cristina , Echenique Laura Matilde 
Published: March 2019

En el presente trabajo nos ocuparemos de la Vejez, la última edad del hombre en Roma en las distintas épocas políticas desde la Monarquía pasando por la República hasta comienzos del Imperio, el enfoque dado es sobre todo recoger las ideas de poetas, historiadores, moralistas, filósofos y literatos, para luego describir la situación jurídica de los Adultos Mayores en nuestra legislación. Palabras Clave: Vejez- Potestad- Adulto Mayor.
Early Detection of Hypoglycemia in Type 1 Diabetes Using Heart Rate Variability Measured by a Wearable Device
Authors: Olde Bekkink Marleen , Koeneman Mats , de Galan Bastiaan E. , Bredie Sebastian J. 
Published: March 2019

OBJECTIVE
Changes in heart rate variability (HRV) occur at the initiation of hypoglycemia due to sympathetic nervous system activity. We investigated the use of HRV detection by a wearable device as an early alert for hypoglycemia.

RESEARCH DESIGN AND METHODS
This proof-of-principle study included 23 patients with type 1 diabetes (14 women, mean age 42 ± 11 years). Patients wore a VitalConnect HealthPatch for 5 days. Hypoglycemia was defined as glucose ≤70 mg/dL (≤3.9 mmol/L) by fingerstick measurement. HRV was analyzed in standardized periods before the hypoglycemic event.

RESULTS
Sixty-six hypoglycemic events were recorded. Hypoglycemia caused a typical HRV pattern in 36 (55%) of the hypoglycemic events. Eighteen events (27%) showed an atypical pattern. Ten events were unclassified (15%), and two did not display a change in HRV (3%).

CONCLUSIONS
Hypoglycemia causes early changes in HRV that can be detected by a wearable device. Measuring real-time HRV seems promising for early hypoglycemia detection.
Natural and anthropogenic conditions of water level fluctuations in lakes – Lake Powidzkie case study (Central-Western Poland)
Authors: Nowak Bogumił M. , Ptak Mariusz 
Published: March 2019

Abstract
The article presents the analysis of water level fluctuations in Lake Powidzkie in the years 1961–2015. The study shows a considerable decrease in mean water levels in the aforementioned multiannual period, averaging 9 cmꞏdecade−1. Such a situation is caused by natural as well as anthropogenic factors, co-determining water relations in the study area. The natural factors include the amount and distribution of precipitation, increase in air temperature and evaporation size, unfavourable relations between the lake and catchment or hydrogeological conditions. Anthropogenic factors particularly include long-term transformations of the natural environment in the region, currently associated with meliorations accompanying the nearby opencast brown coal mines and exploitation of groundwaters for municipal purposes. Water shortages occurring during dry periods were shown not to be compensated in the study area in humid years. This is particularly related to the regional lowering of the aquifer remaining in close relations with Lake Powidzkie. Counteracting the unfavourable hydrological situation is done through hydrotechnical infrastructure which partially limits water outflow from the lake through damming.
Morphological characteristics and lithological conditions of the spring-heads in the Knyszyńska Primeval Forest
Authors: Micun Krzysztof , Roj-Rojewski Sławomir 
Published: March 2019

Abstract
The aim of the study was to determine the morphological characteristics of selected spring-heads in the Knyszyńska Primeval Forest and to identify lithological conditions in areas where groundwater flows to the surface. During the study, detailed bed level measurements of the spring-head areas were conducted. Lidar laser data obtained from the Central Department of Geodetic and Cartographic Documentation in Warsaw were also used for the analysis of morphometry. Based on the data, the detailed contour maps were created in the Surfer 12 programme and the basic parameters of the morphometry of the studied springs were determined. To detect lithological conditions, granulometric analyses were conducted and the filtration coefficient of aquifers in the individual spring-heads was calculated using Hazen and USBSC empirical models. Due to the morphological situation, the examined objects were classified as sub-slope and riverbank spring-heads. In terms of shape, spring-head alcoves are classified as basin-shaped, bowl-shaped and spindle-shaped alcoves. Different morphological processes prevail in each of these types. Basin-shaped alcoves are formed mainly by lateral erosion, bowlshaped alcoves by seepage erosion, landsliding and accumulation in the bottom, spindle-shaped alcoves by seepage erosion, headward erosion, breaking and collapsing. In the investigated outflows of groundwater aquifers are sands and glacifluvial sands with gravel of varying grain size. The lithological variation of aquifers in the spring-heads, directly affects the rate of groundwater filtration in different parts of the alcoves, which in turn leads to different morphogenetic processes and results in changes in the morphology of the spring-head alcoves.
Water supply performance evaluation of the public service of drinking water Case study: East of Algeria
Authors: Boutebba Khereddine , Bouamrane Ali , Ganaoui Nawel , Bouziane Mohamed T. 
Published: March 2019

Abstract
This article presents the results of a survey using a questionnaire conducted in Algeria, to evaluate the quality of public drinking water service, and at the same time the level of dissatisfaction / satisfaction of the subscribers. A statistical analysis of the data has been conducted on a representative sample of general population to identify weaknesses and strengths related to the current state of water management. The assessment of the quality of service, and the quality of water, as well as the price of water under the influence of the geographical distribution of the population was also studied.
The results of the statistical analyses show a wide variability regarding the level of dissatisfaction/satisfaction of the subscribers. In order to simplify the interpretation, we gathered the results in the form of classes and groups in such way that the cities appear on GIS mapping software, with their inhabitants’ degree of satisfaction.
Predicting water erosion in arid lands using the GIS-based RUSLE model: A case study of Bedour catchment, central Tunisia
Authors: Bedoui Chokri 
Published: March 2019

Abstract
This study was conducted with a view to quantifying soil erosion in arid lands of Tunisia. To do this, we have opted to use the RUSLE model based on geographic information systems. By collecting data on rainfall, soils, vegetation, slopes and conservation practices separately as a layer and determining the pixel values for each of these factors, a quantified assessment of erosion in the basin is obtained. The data superposition and computing, following the model equations and protocol, allowed us to know the spatialized water erosion values at the pixel level. For the whole catchment, the study showed values oscillating between 0 and 163 Mgꞏha−1ꞏyear−1 with an average annual rate of 3 Mgꞏha−1ꞏyear−1. With such a low R (rainfall erosivity) factor (between 21.43 and 21.88 MJꞏmmꞏha–1ꞏh–1ꞏyear–1) itself related to low monthly and annual rainfall amounts, the region experiences locally very high annual erosion rates. Soil protection through conservation practices has saved the basin from even higher erosion. While plains cultivated and equipped with contour benches often suffer from low rates of erosion (less than 2 Mg·ha–1·year–1), unused slopes are neglected without protection, resulting in significantly high rates of erosion.
Hydro-chemical and microbiological characterization of Lower Cretaceous waters in a semi-arid zone Beni-Ounif syncline, South-West of Algeria
Authors: Merzougui Fatima Zahra , Makhloufi Ahmed , Merzougui Touhami 
Published: March 2019

Abstract
The article analyses the water quality of the Lower Cretaceous aquifer in the Beni-Ounif syncline. To this end, 42 samples were taken for physico-chemical analysis and 28 for microbiological analysis in March, May and October 2017 from 14 sampling points. The results of physico-chemical analysis were processed by multi-variety statistical analysis methods: principal component analysis (PCA) and hierarchical cluster analysis (HCA) coupled to hydro chemical methods: Piper diagram.
The PCA allowed us to explore the connections between physico-chemical parameters and similarities between samples and to identify the most appropriate physico-chemical elements to describe water quality.
The HCA allowed us to classify the sampling points according to the similarity between them and thus reduce them for the next follow-up analysis.
Waters of the syncline are characterized by medium to low mineralization (320 < EC < 7600 μS∙cm–1 and 200 < RS < 4020 mg∙dm−3) and hardness of between 22 and 123°f. Only 19% of the samples show NO3 concentrations exceeding the Algerian standards.
Microbiologically, the study reports the presence of bacteria: coliforms (<8 CFU∙0.1 dm−3), Streptococcus D (<1100 CFUꞏ0.1 dm–3), Clostridium sulphito-reducer of vegetative form (<90 CFUꞏ0.02 dm–3) and sporulate (<4 CFUꞏ0.02 dm–3), total aerobic mesophilic flora at 22°C (<462 CFUꞏ0.001 dm–3) and at 37°C (<403 CFUꞏ0.001 dm–3). It must be noted that no presence of thermo-tolerant coliforms is observed.