bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic basis of immunity in Indian cattle as revealed by comparative analysis ofBosgenome
Authors: Thambiraja Menaka , Iyengar Shukkruthi K. , Satishkumar Brintha , Kavuru Sai Rohith , Katari Aakanksha , Singh Dheer , Onteru Suneel K. , Yennamalli Ragothaman M. 
Published: December 2024

AbstractIndian cattle breeds (Bos indicus) are renowned for their greater resilience compared toBos taurusin diverse environments and resistance to infections. However, the genomic signatures associated with disease-resistance traits in Indian cattle remain underexplored. A genome-wide comparison betweenBos indicusandBos tauruscould uncover key immune markers related to disease-resistance traits. We conducted chromosome-by-chromosome analyses among Bos genomes using three pairwise combinations, namelyBos taurus(Hereford) vs.Bos indicus(Nelore breed),Bos taurus(Hereford) vs.Bos indicus(Gir), andBos indicus(Nelore) vs.Bos indicus(Gir), to investigate genomic variations in immunity genes and quantitative trait loci (QTLs). The whole genome for Nelore, Gir, and Hereford breeds were compared using bioinformatics tools: SyMAP, GSAlign, and SyRI. Non-syntenic regions were associated with four immunity genes in Nelore: VSTM1L, Galectin-9, HDAC5, and POLH. Significant genomic differences were observed on chromosomes 1 and X across all inter-breed comparisons. Chromosome X of Nelore lost 50 Mbps of genomic regions compared to those of Hereford, although all genes remained intact. Further exploration of translocation events on Chromosome X in Nelore revealed chromosomes 13 and 11 had more single nucleotide variants and insertions, respectively. In total, 8, 16, and 209 innate immunity genes in Nelore were impacted by longer insertions, deletions, and substitutions respectively, and 62 innate immunity genes were affected by all three structural variations. QTL analysis indicated these variations were primarily linked to milk and health traits. This study highlights genomic variations in innate immunity genes in Nelore and associated QTLs.
Transcription factors form a ternary complex with NIPBL/MAU2 to localize cohesin at enhancers
Authors: Fettweis Gregory , Wagh Kaustubh , Stavreva Diana A. , Jiménez-Panizo Alba , Kim Sohyoung , Lion Michelle , Alegre-Martí Andrea , Rinaldi Lorenzo , Johnson Thomas A. , Krishnamurthy Manan , Wang Li , Ball David A. , Karpova Tatiana S. , Upadhyaya Arpita , Vertommen Didier , Recio Juan Fernández , Estébanez-Perpiñá Eva , Dequiedt Franck , Hager Gordon L. 
Published: December 2024

ABSTRACTWhile the cohesin complex is a key player in genome architecture, how it localizes to specific chromatin sites is not understood. Recently, we and others have proposed that direct interactions with transcription factors lead to the localization of the cohesin-loader complex (NIPBL/MAU2) within enhancers. Here, we identify two clusters of LxxLL motifs within the NIPBL sequence that regulate NIPBL dynamics, interactome, and NIPBL-dependent transcriptional programs. One of these clusters interacts with MAU2 and is necessary for the maintenance of the NIPBL-MAU2 heterodimer. The second cluster binds specifically to the ligand-binding domains of steroid receptors. For the glucocorticoid receptor (GR), we examine in detail its interaction surfaces with NIPBL and MAU2. Using AlphaFold2 and molecular docking algorithms, we uncover a GR-NIPBL-MAU2 ternary complex and describe its importance in GR-dependent gene regulation. Finally, we show that multiple transcription factors interact with NIPBL-MAU2, likely using interfaces other than those characterized for GR.
Modulation of Purkinje Cell Inhibition by Stem Cell Factor
Authors: Zaman Tariq , Lahr Jessica , Cherian Suraj , Pozzo-Miller Lucas , Williams Michael R 
Published: December 2024

Abstract / SummaryTarget derived factors influence the specification, maintenance, and modulation of synaptic connectivity. The transmembrane protein, Kit Ligand, and Kit receptor tyrosine kinase are differentially expressed in connected neurons. In development and postnatal periods, these proteins maintain connectivity between cerebellar Purkinje cells (PC) that express Kit Ligand, and presynaptic Molecular Layer Interneurons (MLI) expressing Kit. In this study, it is demonstrated that Stem Cell Factor (SCF), the active extracellular domain of Kit Ligand, produces a potent potentiation of inhibition upon Purkinje Cells. The SCF enhancement of inhibition required presynaptic Kit, produced long term suppression of PC firing, and was associated with a specific potentiation of basket cells of the MLI1 subtype. It is posited that SCF exerts a postsynaptic effect involving enhanced sensitivity of somatic PC GABAAreceptors. This work demonstrates that the SCF/Kit axis modulates synaptic function in adult tissue.
Competition between glycine and GABAAreceptors for gephyrin controls their equilibrium populations at inhibitory synapses
Authors: Kostrz Dorota , Maynard Stephanie A , Schulte Clemens , Laurent François , Masson Jean-Baptiste , Maric Hans M , Gosse Charlie , Triller Antoine , Strick Terence R , Specht Christian G 
Published: December 2024

AbstractGlycine and GABA receptors are ligand-gated chloride channels that mediate inhibitory neurotransmission throughout the central nervous system. The receptors co-localise widely at inhibitory synapses in the spinal cord and in the brainstem due to their interaction with an overlapping binding site of the synaptic scaffold protein gephyrin, pointing to a direct competition between the different receptor types. We have put this hypothesis to the test using single molecule approaches to measure receptor-gephyrin interactions in cells andin vitro. We explored the effects of receptor competition at inhibitory synapses in living neurons by measuring the change in the accumulation and effective stabilisation energy of glycine receptors in the presence of interfering GABA receptor complexes through single molecule tracking and diffusion analysis. Secondly, using molecular tweezers, we quantified the thermodynamic properties of receptor-gephyrin binding, demonstrating direct and reversible competition through the addition of interacting peptides in solution. The relatively low affinity of GABA receptor subunits for gephyrin compared to the glycine receptor raises interesting questions about the role of this competition in synaptic plasticity. We hypothesize that GABA and glycine receptor competition constitutes a molecular system designed to reconcile synapse stability and plasticity at mixed inhibitory synapses.
Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail
Authors: Zhang Daniel X. , Isupov Michail N. , Davies Rebecca M. , Schwarzer Sabine , McLaren Mathew , Stuart William S. , Gold Vicki A.M. , Oksanen Hanna M. , Quax Tessa E.F. , Daum Bertram 
Published: December 2024

AbstractOutnumbering their hosts by at least a factor of 10, viruses are the most common biological entity on Earth, are major drivers of evolution, and greatly impact on the dynamics of our planet’s ecosystems. While viruses infecting bacteria and eukaryotes have been extensively studied, the viruses roaming the archaeal domain remain largely unexplored. In recent years, a growing number of archaeal viruses have been described, revealing a stunningly diverse range of morphologies that appear unique to archaea. Detailed structural studies are paramount to fully understand how archaeal viruses infect their hosts. However, no complete atomic models of archaeal viruses are available to date. Using electron cryo-microscopy, we investigated the structure of the archaeal virus Haloferax tailed virus 1 (HFTV1), which infects the halophile Haloferax gibbonsii LR2-5 originating from the Senegalese salt lake Retba. Through single particle analysis, we achieved near-atomic resolution for the entire set of HFTV1’s structural proteins, enabling the building of a full atomic model of the virion. Comparing the structures of DNA filled and empty capsids, we visualise structural changes occurring upon DNA ejection. By investigating the double-stranded DNA inside the capsid, we elucidate how the genome is spooled upon loading. Furthermore, our structure reveals putative cell-surface receptor-binding and catalytic roles of capsid turret, baseplate, and tail fibre proteins. Together, our data provide new insights into the mechanisms of HFTV1 assembly and infection, unveiling new perspectives on general rules of host-virus interactions in archaea and their evolutionary links to bacterial and eukaryotic viruses.
Qualitative modeling of factors affecting auditor’s judgment and decision-making
Authors: Shamsadini Kazem , Javanmard Hadis , Morady Zohier 
Published: December 2024

Purpose
The purpose of this paper is to provide a qualitative model in the field of factors affecting auditors’ judgment and decision-making. The current research follows a descriptive survey method.

Design/methodology/approach
A survey method was used to conduct this research, with a questionnaire as the primary tool. The statistical population consists of auditors with extensive knowledge and opinions on the research problem and its theoretical foundations. A sample of eight auditors was selected using the snowball sampling method.

Findings
The research results indicated that factors influencing auditors’ judgment and decision-making include reviewing audit reports from past periods, the knowledge and expertise of audit partners, input from the audit team, understanding social, cultural, political and economic environmental issues, auditor independence, technical and specialized skills of auditors, increasing experience, knowledge of business owners’ activities, rising audit fees and inhibiting forces in auditors’ judgment and decision-making. These inhibiting forces include lack of client knowledge, emotions, mental states, ethical considerations, business unit ownership structure, complexity of reviewed unit activities, state-owned business unit ownership, lack of understanding of employer activities and lack of expertise in the employer’s industry.

Practical implications
The model presented in this research aids in enhancing and refining the judgment and decision-making of independent auditors, resulting in more favorable financial reports for report users. Additionally, the theory developed through this research method can lead to the formulation of hypotheses that future researchers can test.

Originality/value
Auditor judgment is a vital aspect of independent audits, emphasized in auditing standards and professional codes of conduct.
Chatbots in complaint handling: the moderating role of humor
Authors: Park Jae Eun , Fan Alei , Wu Laurie 
Published: December 2024

Purpose
Artificial intelligence (AI) powered chatbot technology is increasingly used to handle customer complaints in the service recovery process. Built on the justice theory, this paper aims to explore the optimal way to utilize chatbots in handling customer complaints for service failure recovery and the moderating role of humor.

Design/methodology/approach
Two scenario-based between-subjects experimental design studies were conducted to examine the two aspects of justice in service failure recovery: Study 1 focuses on procedural justice manifested as perceived control and Study 2 on interactional justice assessed as social presence. Furthermore, the moderating role of humor is investigated.

Findings
The results indicate that both perceived control and social presence can improve chatbots’ effectiveness in handling service failures to regain customer satisfaction and the consequent revisit intention. However, humor shows opposite effects in the two studies: chatbots using humorous language in complaint handling may attenuate the positive effect of perceived control but enhance the positive effect of social presence.

Practical implications
The findings provide practical guidelines to the service industry regarding the optimal service design and technology deployment in customer service, particularly for an effective service failure recovery process.

Originality/value
This research contributes to the service failure recovery literature by investigating how the new service technology of AI-powered chatbots can effectively handle customer complaints for service failure recovery. Furthermore, the research reveals the nuanced effects of humor in different complaint-handling situations.
Exploring the effect of psychosocial safety climate on employee outcomes: the moderating roles of workload and organizational training
Authors: ZamirKhan Abu Daud Lutful , Rubel Mohammad Rabiul Basher , Kee Daisy Mui Hung 
Published: December 2024

PurposeThis research aims to investigate the impact of psychosocial safety climate (PSC) on employee quitting intention (QI) and organizational citizenship behavior (OCB), considering the moderating effects of workload and organizational training.Design/methodology/approachThe research adopts a cross-sectional approach, involving 151 employees from the apparel manufacturing industry in Bangladesh who participated in the survey. Data analysis is conducted using SmartPLS.FindingsThe study reveals that PSC contributes to prolonged employee tenure and fosters positive organizational citizenship behavior. Workload and organizational training play significant moderating roles, influencing the negative and positive effects of PSC on QI and OCB, respectively.Research limitations/implicationsWhile the PSC model has been studied, there are ample opportunities to enhance and validate theoretical models exploring the moderating impact of job demands and resources on PSC-outcome relationships.Originality/valueThis study serves as an exploration of the influence of working conditions on the outcomes of PSC within the context of the manufacturing industry in a non-Western developing country like Bangladesh. Moreover, it looks into the moderating roles of workload and organizational training to extend the PSC model.
Triple Nexus: leveraging development supply chains for humanitarian aid and peace in Southeast Asia – a GBV focus
Authors: Plesner Volkdal Christina 
Published: December 2024

Purpose
The purpose of this study is to emphasize the crucial role of supply chains in humanitarian and development interventions, specifically targeting gender-based violence in East Asia and the Pacific, regions prone to climate change-induced disasters. It explores optimizing development supply chains for humanitarian use, and the plausible impacts on community peacebuilding.

Design/methodology/approach
By adopting an abductive approach to the action research methodology, based on 11 case studies, including intensive fieldwork in seven, this study evaluates development supply chains for their adaptability, institutionalization and flexibility to support humanitarian needs and analyzes the potential interlinkages of peacebuilding.

Findings
The research uncovers the irregular and unsystematized present humanitarian efforts and highlighting a lack of advancement in development channels for implementing effective humanitarian preparedness and response. By focusing on the Triple Nexus framework and based on the findings, the study rather proposes enhancing the humanitarian supply chain, suggesting a restructured mechanism for development channels that fosters synergies across the dimensions. It intricately connects peacebuilding applying a Triple Nexus Analytical Framework, examining the potential impact on micro-level peacebuilding outcomes.

Research limitations/implications
The research recognizes the challenge in conceptualizing peacebuilding within the Triple Nexus framework, calling for a nuanced understanding of peacebuilding across different levels. Contrary to expectations, the case studies yielded unexpected results, suggesting a need for a reversed approach in the applied methodology, hence advancing humanitarian supply chains with synergies to development channels that in the specific cases studies lack efficiency in their current set-up.

Originality/value
This study examines the collaboration between humanitarian efforts and development initiatives and how they can collectively contribute to peacebuilding. It pushes forward the conversation on improving humanitarian preparedness by leveraging development supply chains and explores the effects of peacebuilding on local levels.
Unveiling redundancy in environmental, social, and governance reporting framework in India: an exploratory study
Authors: Devi Renu , Firoz Mohammad , Saravanan R. 
Published: December 2024

Purpose
This study aims to investigate redundant information in mandatory non-financial reports (NFRs) demanded by regulators, focusing primarily on overlapping disclosures in a new Indian sustainability reporting (SR) framework.

Design/methodology/approach
The study sample comprised NIFTY100 listed entities that published SR voluntarily during 2021–2022. The authors used content analysis and cosine similarity techniques to conceptually compare redundancy in SR disclosures with non-financial disclosures.

Findings
The findings reveal an information overlap in SR disclosure with other NFRs disclosures. The disclosures of Directors’ Report have higher cosine similarity scores at the firm level with SR, followed by the Management Discussion and Analysis report, Corporate Governance report and Corporate Social Responsibility report. The additional analysis reveals that qualitative disclosures and disclosures comprising governance factors overlap more in SR.

Practical implications
Policymakers should look to establish relevant disclosure guidelines in the SR system, and thereby, shed light on fundamental issues to enhance future SR framework reforms.

Social implications
The study highlight the need for integration and amendment in the disclosure guidelines of NFRs to improve the overall transparency of the reports.

Originality/value
Previous studies have examined the redundancy in annual reports and SRs from the point of view of overlapping information. To the best author’s knowledge, this is possibly among the first studies to offer insights into the repetition of disclosures required by regulators in statutory NFRs based on environmental, social, and governance factors through the lenses of the institutional theory.
Reported foodservice environmental sustainability practices in Australian healthcare and aged care services pre and post the onset of COVID‐19
Authors: MacKenzie‐Shalders K. , Higgs J. , Cruickshank D. , Tang X. , Collins J. 
Published: December 2024

AbstractAimsHealthcare foodservices substantially impact global environmental changes. This study investigated environmentally sustainable practices in Australian health foodservices; and perceptions of the influence of COVID‐19 on foodservice environmental sustainability.MethodsAn observational study was undertaken collecting data at two time points (2019 and 2022) via a pre‐workshop survey with healthcare foodservice stakeholders. The survey used rating scales and free text options to explore sustainable practices, perceived barriers and enablers to sustainable practices, and a free‐text response on the impact of COVID‐19. Analysis included independent samples t‐tests (continuous, normally distributed), Mann–Whitney U tests (continuous non‐normally distributed data), and Pearson chi‐squared tests (categorical data). A qualitative analysis of free text responses to a single question about the impact of COVID‐19 was used to identify, analyse, and report positive and negative aspects of COVID‐19 for sustainable foodservice practices.ResultsDemographic and employment characteristics were similar between timepoints n = 37 (2019) and n = 30 (2022), except for number who had attended sustainability training (n = 14, 38.8% vs n = 19, 63.3%; p = 0.038). There were fewer private hospital (n = 6, 16.2% vs n = 0), (0%) and more rural site representation in 2022 (n = 2, 5.4% vs n = 13, 43.3%; p <0.001). Sustainable foodservice practices were consistent across timepoints (overall mean (SD) sum score for sustainable practices 63.3 (20.7) vs 61.3 (20.4); p = 0.715), with recycling cardboard (n = 27, 90.0% vs n = 22, 84.6%), and the use of reusable cutlery (n = 26, 86.7% vs n = 22, 84.6%) the most prevalent practices at both timepoints. A ‘lack of equipment’ was the primary reported barrier while passionate staff (“champions”) was the primary reported enabler. Participants reported that the COVID‐19 pandemic led to an increase in single‐use disposable items.ConclusionsThis study describes negligible changes in reported environmental sustainability practices by Australian healthcare foodservice stakeholders from 2019 to 2022. The study provides useful information on sustainability beliefs and practices in healthcare foodservices.
Improving food safety compliance of potential employees through a novel model of knowledge, attitude, commitment, and practice
Authors: Taha Sadi , Angor Malak , Al‐Marazeeq Khaled M. , Osaili Tareq M. , Albloush Ahmad , Al‐Rousan Walid M. , Ajo Radwan , Holley Richard , Fadhel Arif , Alboqai Omar 
Published: December 2024

AbstractThe global burden of foodborne illnesses remains high due to consumption of contaminated food. Vigilant attention from all, particularly food handlers, is needed to address food safety concerns and minimize the risks that affect consumer health, international business, and tourism worldwide. The main objectives of this study were to: (i) evaluate the food safety knowledge, attitudes, commitment (involving affective, continuance, and normative types), and practices of students studying subjects related to food sciences in Jordanian universities and (ii) examine the impact of knowledge and attitude on commitment, and subsequently, on relevant practices using structural equation modeling. The questionnaire was completed by 526 students from 5 universities. The score percentages of students in tested categories were as follows: agreement on food safety knowledge (87.7%), attitude (92%), affective commitment (90.4%), continuance commitment (83%), normative commitment (90.3%), and practices (91.5%). The results showed that associations between food safety knowledge and attitude with the three types of commitment were positive and significant (p < 0.05). Affective and normative commitments acted as partial mediators between food safety knowledge and practices and between food safety attitude and practices, whereas continuance commitment did not act as a mediator. Furthermore, a moderate correlation was found between food safety knowledge and attitude. Focus on food safety commitment drivers is essential, as commitment plays a key role in achieving actual hygienic practices by translating adequate knowledge and positive attitudes to improvements in hygienic behavior and reductions in the risk of foodborne illnesses.