bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diverse Mechanisms of Resistance in Carbapenem-Resistant Enterobacteriaceae at a Health Care System in Silicon Valley, California
Authors: Senchyna Fiona , Gaur Rajiv , Sandlund Johanna , Truong Cynthia , Tremintin Guillaume , Küeltz Dietmar , Gomez Carlos A. , Tamburini Fiona B. , Andermann Tessa , Bhatt Ami , Tickler Isabella , Watz Nancy , Budvytiene Indre , Shi Gongyi , Tenover Fred C. , Banaei Niaz 
Published: April 2018

AbstractCarbapenem-resistant Enterobacteriaceae (CRE) are emerging as a major health threat in North America. The mechanism of resistance to carbapenems has therapeutic and public health implications. We comprehensively characterized the underlying mechanisms of carbapenem resistance in CRE isolates recovered between 2013 and 2016 at a health system in Northern California. Genotypic methods were used to detect carbapenemases and plasmid-encoded cephalosporinases, and mass spectrometry was used to quantify relative porin levels for OmpC and OmpF and their analogs. MICs for imipenem-relebactam, meropenem-vaborbactam, ceftazidime-avibactam, and ceftolozane-tazobactam were measured. Whole genome sequencing was used for strain typing. A carbapenemase gene encoding blaOXA-48 like, blaNDM, blaKPC, blaSME, blaIMP, and blaVIM was detected in 38.7% (24/62) of CRE isolates. Porin levels was down at least 2-fold in 91.9% (57/62) of isolates. Including carbapenemase genes and porin loss, the mechanism of resistance was identified in 95.2% (59/62) of CRE isolates. Of the carbapenemase gene-positive isolates, blaKPC -positive isolates were 100% susceptible to ceftazidime-avibactam, meropenem-vaborbactam, and imipenem-relebactam; blaOXA-48 like-positive isolates were 100% susceptible to ceftazidime-avibactam; and blaSME-positive isolates were 100% susceptible to meropenem-vaborbactam and ceftolozane-tazobactam. 100% (38/38), 92.1% (35/38), 89.5% (34/38), and 31.6% (12/38) of carbapenemase gene-negative CRE isolates were susceptible to ceftazidime-avibactam, meropenem-vaborbactam, imipenem-relebactam, and ceftolozane-tazobactam, respectively. None of the CRE strains were genetically identical. In conclusion, at this health system in Silicon Valley, carbapenemase-producing CRE occurred sporadically and were mediated by diverse mechanisms. Nucleic acid testing for blaOXA-48 like, blaNDM, blaKPC, blaIMP, and blaVIM was sufficient to distinguish between carbapenemase-producing and non-producing CRE and accurately predicted susceptibility to ceftazidime-avibactam, meropenem-vaborbactam and imipenem-relebactam.
Improved CD4 T-cell profile and inflammatory levels in HIV-infected subjects on maraviroc-containing therapy is associated with better responsiveness to HBV vaccination
Authors: Herrero-Fernéndez Inés , Rosado-Sánchez Isaac , Genebat Miguel , Tarancón-Díez Laura , Rodríguez-Méndez María Mar , Pozo-Balado María Mar , Lozano Carmen , Ruiz-Mateos Ezequiel , Leal Manuel , Pacheco Yolanda M. 
Published: April 2018

ABSTRACTIntroductionWe previously found that a maraviroc-containing combined antiretroviral therapy (MVC-cART) was associated with a better response to the Hepatitis B Virus (HBV) vaccine in HIV-infected subjects younger than 50 years old. We aimed here to extend our previous analysis including immunological parameters related to inflammation, T-cell and dendritic cell (DC) subsets phenotype and to explore the impact of MVC-cART on these parameters.MethodsWe analyzed baseline samples of vaccinated subjects under 50 years old (n=41). We characterized CD4 T-cells according to the distribution of their maturational subsets and the expression of activation, senescence and prone-to-apoptosis markers; we also quantified Treg-cells and main DC subsets. Linear regressions were performed to determine the impact of these variables on the magnitude of vaccine response. Binary logistic regressions were explored to analyze the impact of MVC-cART on immunological parameters. Correlations with the time of MVC exposure were also explored.ResultsMVC-cART remained independently associated with HBV-vaccine responsiveness even after adjusting by immunological variables. The %CD4+CD25hiFoxP3+ki67+ and %pDCs were also independently associated. Moreover, HIV-infected subjects on MVC-containing therapy prior to vaccination showed lower inflammatory levels, activated CD4 T-cells and frequency of Treg cells and higher frequency of recent thymic emigrants.ConclusionTreg-cell levels negatively impacted the HBV-vaccine response, whereas higher pDCs levels and a MVC-cART prior to vaccination were associated with better responsiveness. These factors together with the improved phenotypic CD4 T-cell profile and the lower inflammatory levels found in subjects with a MVC-cART prior HBV vaccination could contribute to their enhanced vaccine response.
Label-free visualization of type IV pili dynamics by interferometric scattering microscopy
Authors: Tala Lorenzo , Fineberg Adam , Kukura Philipp , Persat Alexandre 
Published: April 2018

AbstractType IV pili (TFP) are slender objects that assemble by polymerization and secretion of protein subunits from bacterial cell surfaces. The mechanisms by which these surface structures of microscopic length and molecular diameter modulate the physical interaction of bacteria with their environment, however, remains poorly understood largely due to limitations in our ability to monitor and characterize the dynamics of individual TFP. Here, we demonstrate that interferometric scattering microscopy (iSCAT) enables label-free and dynamic visualization of TFP in intact cells of the opportunistic pathogenPseudomonas aeruginosa. As a result, we can directly monitor extension, attachment and retraction events on millisecond timescale and nanometer length scale in three dimensions. These capabilities allow us to observe thatP. aeruginosais able to crawl against the direction of flow using short TFP filaments. Also, careful observation show that TFP retract rapidly after surface attachment, suggesting thatP. aeruginosasenses contact of the pilus tip with the solid substrate. These results illustrate the power of iSCAT for the label-free visualization of small, dynamic microbial extracellular structures.
How to overcome the ATRA resistance with the 9aaTAD activation domains in retinoic acid receptors
Authors: Piskacek Martin , Havelka Marek , Knight Andrea 
Published: April 2018

AbstractIn higher metazoa, the nuclear hormone receptors activate transcription trough their specific adaptors, nuclear hormone receptor cofactors NCoA, which are surprisingly absent in lower metazoa. In this study, we demonstrated that the 9aaTAD from NHR-49 receptor activates transcription as a small peptide. We showed, that the 9aaTAD domains are conserved in the human nuclear hormone receptors including HNF4, RARa, VDR and PPARg. The small 9aaTAD peptides derived from these nuclear hormone receptors also effectively activated transcription and that in absence of the NCoA adaptors. We identified adjacent inhibitory domains in the human HNF4 and RARa, which hindered their activation function.In acute promyelocytic leukaemia (PML-RARa), the receptor mutations often caused all-trans retinoic acid (ATRA) resistance. The fact that almost the entire receptor is needed for ATRA mediated receptor activation, this activation pathway is highly susceptible for loss of function when mutated. Nevertheless in the most of the reported mutants, the activation domains 9aaTAD are still intact. The release of activation 9aaTAD from its dormancy by a new drug might be the sound strategy in combat the ATRA resistance in PML leukaemia.Graphical Abstract
Extensive cellular heterogeneity of X inactivation revealed by single-cell allele-specific expression in human fibroblasts
Authors: Garieri Marco , Stamoulis Georgios , Falconnet Emilie , Ribaux Pascale , Borel Christelle , Santoni Federico , Antonarakis Stylianos E. 
Published: April 2018

ABSTRACTIn eutherian mammals, X chromosome inactivation (XCI) provides a dosage compensation mechanism where in each female cell one of the two X chromosomes is randomly silenced. However, some genes on the inactive X chromosome and outside the pseudoautosomal regions escape from XCI and are expressed from both alleles (escapees). Given the relevance of the escapees in biology and medicine, we investigated XCI at an unprecedented single-cell resolution. We combined deep single-cell RNA sequencing with whole genome sequencing to examine allelic specific expression (ASE) in 935 primary fibroblast and 48 lymphoblastoid single cells from five female individuals. In this framework we integrated an original method to identify and exclude doublets of cells. We have identified 55 genes as escapees including 5 novel escapee genes. Moreover, we observed that all genes exhibit a variable propensity to escape XCI in each cell and cell type, and that each cell displays a distinct expression profile of the escapee genes. We devised a novel metric, the Inactivation Score (IS), defined as the mean of the allelic expression profiles of the escapees per cell, and discovered a heterogeneous and continuous degree of cellular XCI with extremes represented by “inactive” cells, i.e., exclusively expressing the escaping genes from the active X chromosome, and “escaping” cells, expressing the escapees from both alleles. Intriguingly we found that XIST is the major genetic determinant of IS, and that XIST expression, higher in G0 phase, is negatively correlated with the expression of escapees, inactivated and pseudoautosomal genes. In this study we use single-cell allele specific expression to identify novel escapees in different tissues and provide evidence of an unexpected cellular heterogeneity of XCI driven by a possible regulatory activity of XIST.
Implementing internal environmental management and voluntary environmental disclosure
Authors: Passetti Emilio , Cinquini Lino , Tenucci Andrea 
Published: April 2018

Purpose
The purpose of this paper is to investigate to what extent the implementation of internal environmental management and voluntary environmental information is related to organisational change.

Design/methodology/approach
Organisational change literature provided a framework for the analysis of the materials which were collected through a mixed method. Data on internal environmental management were collected through a survey, while a quality disclosure index was used to assess the quality of the environmental voluntary disclosure. Interviews were used to enhance the quantitative results interpreted according to the four pathways proposed by Tilt (2006) and characterised by several levels of internal environmental management and voluntary disclosure.

Findings
The results indicated that companies implement more internal activities than external disclosure. Environmental planning and operational practices were the most important changes carried out. When environmental management accounting and environmental disclosure were also implemented, environmental aspects were more integrated within companies, thus revealing that a more structured integration of sustainability aspects within organisational values had taken place. The results underline the importance of primarily establishing a set of internal changes, driven by environmental planning, to promote organisational change.

Research limitations/implications
The study presents a larger empirical analysis of the organisational change pathways followed by companies, showing similarities and differences among the four pathways. The results underline the importance of both dimensions for studying organisational changes. The framework of Tilt has been enriched, considering a more precise explanation of the internal aspects and adding the concept of the quality of disclosure as proxy to assess organisational change.

Originality/value
Organisational change is investigated through an extensive analysis of internal and external aspects and collecting quantitative and qualitative evidence. The analysis complements previous sustainability accounting literature focussed on the analysis of internal environmental management and external disclosure.
Statistical analysis of the crowd dynamics in Al-Masjid Al-Nabawi in the city of Medina, Saudi Arabia
Authors: Al-Ahmadi Hassan M. , Alhalabi Wael S. , Malkawi Rezqallah Hasan , Reza Imran 
Published: April 2018

Purpose
The purpose of this study is to analyze the crowd dynamics of the visitors at Al-Masjid al-Nabawi during the most oversaturated period to characterize the most critical conditions and suggest technical solutions to accommodate visitors and provide them safe passage.

Design/methodology/approach
In this study, the time of entrance from the Al-Salam Gate to the tomb and from the tomb to the exit from the Al-Baqi’ Gate has been collected in the most oversaturated period. To be precise and to model the worst case, important crowd measures of effectiveness data are collected in the two holiest times considered by Muslims, during the holy month of Ramadan and the month of Dhul-Hijjah and during the busiest hours of the day to consider safety factors while proposing future solutions. The conventional manual head-counting method has been adopted to determine the crowd density and to carry out actual counting of the visitors from the recorded videos and photos captured by the legitimate authority.

Findings
The analyses revealed that the crowd dynamics in the month of Ramadan (peak) are statistically different from those for other times (off peak). In general, the crowd dynamics at all times on days other than Ramadan are almost identical.

Originality/value
The results of crowd characterization from this study are expected to help optimize crowd management in the Masjid at the most critical location and time. The data collected in this study could be used for future research to simulate similar crowd scenes or for even different crowd management scenarios in case of emergencies such as fire hazards or evacuation process.
Structural modelling of multi-thread fancy yarn
Authors: Alshukur Malek , Gong Hugh , Stylios George 
Published: April 2018

Purpose
The purpose of this paper is to mathematically model the structure of doubled fancy yarns made by combining together several threads.

Design/methodology/approach
It was assumed that such a structure may have two distinctive parts – sinusoidal and helical (i.e. sigmoidal). This model is based on calculating the length of the effect thread in relation to the core thread. The case of having several variants of such a structure was discussed to account for several types of doubled fancy yarns. The number of wraps of the binder, the overfed ratio, and heights of the fancy profiles in the different parts were the fundamental parameters of this model. The effects of changes in the number of wraps, the overfeed ratio or both simultaneously, on this model, were also considered. The shape factor of fancy yarn was also modelled depending on the basic model of the structure.

Findings
The model was tested and the correlation coefficient between the theoretical value and the real value of length of the effect thread was 0.90.

Originality/value
This model is useful for predicting the length of the effect component based on the type, dimension and number of the fancy profiles of doubled fancy yarn, and for understanding the changes of the multiple-thread structure of fancy yarn when the overfeed ratio and/or the number of wraps were to change.
Prevalence and determinants of food insecurity in migrant Sub-Saharan African and Caribbean households in Ottawa, Canada
Authors: Tarraf Diana , Sanou Dia , Blanchet Rosanne , Nana Constance P. , Batal Malek , Giroux Isabelle 
Published: April 2018

Purpose
Food insecurity (FI) is an important social determinant of health and is linked with higher health care costs. There is a high prevalence of FI among recent migrant households in Canada. The purpose of this paper is to evaluate the prevalence of FI in Sub-Saharan African and Caribbean migrants in Ottawa, and to explore determinants of FI in that population.

Design/methodology/approach
A cross-sectional study was conducted among 190 mothers born in Sub-Saharan Africa or the Caribbean living in Ottawa and having a child between 6 and 12 years old. Health Canada’s Household Food Security Survey Module was used to evaluate participants’ food security in the past 12 months. χ2 tests and multivariate logistic regression analyses were used to measure determinants of FI (n=182).

Findings
A very high rate of FI (45.1 percent) was found among participants. When numerous determinants of FI were included in a multivariate model, household FI was associated with Caribbean origin, low education attainment, lone motherhood, living in Canada for five years or less and reliance on social assistance.

Originality/value
These findings highlight the need for FI to be explicitly addressed in migrant integration strategies in order to improve their financial power to purchase sufficient, nutritious and culturally acceptable foods. Enhancing migrants’ access to affordable child care and well-paid jobs, improving social assistance programs and providing more affordable subsidized housing programs could be beneficial.
The 5S organizational agility framework: a dynamic capabilities perspective
Authors: Baškarada Saša , Koronios Andy 
Published: April 2018

PurposeAlthough there is widespread agreement that organizational agility is increasingly becoming critical to achieving sustained competitive advantage, there is little consensus on what exactly constitutes organizational agility, or how it may be assessed and improved. The purpose of this paper is to contribute toward operationalization of the organizational agility construct through a high-level conceptual framework grounded in dynamic capabilities.Design/methodology/approachThis is a conceptual paper that presents a new organizational agility framework, which draws from, and builds on, the existing literature.FindingsThis paper defines organizational agility as rapid, continuous and systematic evolutionary adaptation and entrepreneurial innovation directed at gaining and/or maintaining competitive advantage. The 5S Organizational Agility Framework proposes five dynamic capabilities (sensing, searching, seizing, shifting and shaping) underpinning organizational agility.Originality/valueThe framework presented in this paper contributes toward operationalization of the organizational agility construct.
Sustainability of indigenous folk tales, music and cultural heritage through innovation
Authors: Chan Clare Suet Ching 
Published: April 2018

PurposeThe aim of producing Bah Luj Production, four folk tales books accompanied by a compact disc of its narration, dialogs and music is to revitalize the folk tales, music and cultural heritage of the indigenous Semai by condensing them into an innovative resource package, tailored to appeal to the current generation’s consumer interests and lifestyles. The targeted audiences for the product are Malaysians, in particular the Semai community, as well as other local and international consumers. The purpose of this paper is to examine a practice-led approach toward considering the empowerment of selected culture bearers assisted by the intervention of researchers from the academia in facilitating the sustainability of indigenous cultural traditions in Malaysia.Design/methodology/approachThis approach is practice-led – Bah Luj Production was developed based on action, reflection, review and revision. This paper provides visibility to the research process, enabling readers to understand the issues, challenges and decision-making processes. The practice-led approach that was used for this project provides a realistic practical guide, bridging the gap between theory and practice. In the conclusion, the authors also bring forth ideas for improvement through reflection, on suggested approaches in ways this research did not manage to pursue.FindingsThis paper proposes three approaches in advocating for cultural sustainability through innovation: collaboration between selected culture bearers and researchers in the academia; indigeneity of cultural expressions and illustrations; and adaptability and relevance to current interests of indigenous people and consumers of indigenous music and literature. This paper argues that it is important for the researcher to navigate research with relevance to the context and situation.Research limitations/implicationsWhile many articles focus on presenting the outcomes of a research project, this paper guides the reader toward understanding the limitations, constraints and negotiations made by the research team during the research and production stages. Transparency in the process of decision making will enlighten readers on realistic, practical approaches as opposed to idealistic theoretical methods.Practical implicationsThis paper argues for sustainability through innovation and posits that cultural heritage practices that continue to be performed are those that are adaptable, flexible to change and open to innovation – therefore maintaining relevance to time, context and consumers. This paper posits that researchers should be flexible and practical in their research actions and avoid generalizations that come out of recent and popular critical theories as the most, or only suitable, approaches for diverse communities.Originality/valueThe development of an alternate approach, theory/concept and guidelines toward sustainability through innovation make this study the first of its kind. This approach integrates tradition with creation.
Age differences in responsiveness to shocking prosocial campaigns
Authors: Albouy Jeanne , Décaudin Jean-Marc 
Published: April 2018

Purpose
This paper aims to study age differences in responsiveness to shocking prosocial campaigns. It specifically investigates the differences between seniors and young adults regarding the persuasive effect of emotional charity campaigns, the intensity of negative emotions and empathy elicited, the affect-based persuasive process leading to the ad effectiveness and the role of perceived self-efficacy.

Design/methodology/approach
Two studies are conducted on seniors (n1 = 173; n2 = 118) and young adults (n1 = 170; n2 = 123) exposed to appeals for “Action against Hunger” incorporating various levels of emotional content.

Findings
The results indicate that seniors are more receptive to emotional campaigns but only for higher emotional ads and only regarding intention to donate. Young adults report lower levels of empathy, and findings do not suggest major age differences in the reported negative emotions after exposure to low or high emotional appeals. The affect-based persuasion differs; young adults are particularly influenced by negative emotions, whereas the persuasive effect on the elderly stems from an empathetic reaction. Self-efficacy moderates the effect of negative emotions only among older participants.

Practical implications
Social marketers are advised to use vivid and realistic stimuli to reach young people and develop these appeals in social media. For an older target audience, however, marketers are advised to use creative components and media support designed to foster personalisation and proximity.

Originality/value
This study provides a better understanding of age-related effects on emotional persuasion, and addresses the specificity of prosocial appeals involving responsiveness to the suffering of others.