bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphatidylethanolamine made in the inner mitochondrial membrane is essential for yeast cytochromebc1complex function
Authors: Calzada Elizabeth , McCaffery J. Michael , Claypool Steven M. 
Published: February 2018

ABSTRACTOf the four separate PE biosynthetic pathways in eukaryotes, one occurs in the mitochondrial inner membrane (IM) and is executed by phosphatidylserine decarboxylase (Psd1p). Deletion of Psd1, which is lethal in mice, compromises mitochondrial function. We hypothesize that this reflects inefficient import of non-mitochondrial PE into the IM. To test this, we re-wired PE metabolism in yeast by re-directing Psd1p to the outer mitochondrial membrane or the endomembrane system. Our biochemical and functional analyses identified the IMS as the greatest barrier for PE import and demonstrated that PE synthesis in the IM is critical for cytochromebc1complex (III) function. Importantly, mutations predicted to disrupt a conserved PE-binding site in the complex III subunit, Qcr7p, impaired complex III activity similar toPSD1deletion. Collectively, these data demonstrate that PE made in the IM by Psd1p is critical to support the intrinsic functionality of complex III and establish one likely mechanism.
Expanding the genetic toolkit of Tribolium castaneum
Authors: Rylee Johnathan C. , Siniard Dylan J. , Doucette Kaitlin , Zentner Gabriel E. , Zelhof Andrew C. 
Published: February 2018

AbstractThe red flour beetle, Tribolium castaneum, is an important model insect and agricultural pest.However, many standard genetic tools are lacking or underdeveloped in this system. Here, we present a set of new reagents to augment existing Tribolium genetic tools. We demonstrate a new GAL4 driver line that employs the promoter of a ribosomal protein gene to drive expression of a UAS responder in the fat body. We also present a novel dual fluorescent reporter that labels cell membranes and nuclei with different fluorophores for the analysis of cellular morphology. This approach also demonstrates the functionality of the viral T2A peptide for bicistronic gene expression in Tribolium. To facilitate classical genetic analysis, we created lines with visible genetic markers by CRISPR-mediated disruption of the yellow and ebony body color loci with a cassette carrying an attP site, enabling future φC31-mediated integration. Together,the reagents presented here will facilitate more robust genetic analysis in Tribolium and serve as a blueprint for the further development of this powerful model’s genetic toolkit.
Flipped learning and formative evaluation in higher education
Authors: Otero-Saborido Fernando M. , Sánchez-Oliver Antonio J. , Grimaldi-Puyana Moisés , Álvarez-García José 
Published: February 2018

Purpose
The purpose of this paper is to design and validate a continuous self-assessment tool that involves university students in reflection processes on their Flipped Learning model learning.

Design/methodology/approach
For this, 66 students (18.77±1.36) of the first year of the Degree in Physical Activity and Sports Sciences participated for nine weeks in the weekly completion of a self-assessment tool. The questionnaire followed a content validation by a group of experts and, subsequently, reliability was found from the internal consistency perspective through Cronbach’s α.

Findings
The results obtained show a reliable tool that facilitates the work by competencies in university education under the Flipped Learning model.

Originality/value
This work is the first step that responds to the almost non-existent practices of democratic evaluation in Higher Education. The design and validation of questionnaires that consider the measures adopted by the European Higher Education Area and that takes into account European Credit Transfer and Accumulation System is scarce.
The thematic landscape of literature in sustainable supply chain management (SSCM)
Authors: Roy Vivek , Schoenherr Tobias , Charan Parikshit 
Published: February 2018

PurposeThe purpose of this paper is to comprehensively review the vast literature on sustainable supply chain management (SSCM), with the specific objective of a thematic exploration of the literature in order to explicate the principal facets of SSCM development.Design/methodology/approachThis comprehensive review follows the systematic literature review approach.FindingsThe authors find SSCM to develop around five principal facets. The first facet is adoption, which accounts for the development of preparatory grounds – for facilitating the generation of a SSCM philosophy to gradually seep into the frame of traditional supply chain management (SCM). The second facet of implementation accounts for the manifestation of a SSCM-oriented transformation for producing gradual upgrades in the traditional SCM environment. The third facet of extension signifies the broadening of the scope of implementation at a more wider (supply chain) level. The fourth facet of maintenance outlines the need for ensuring the continuity of progress in the course of SSCM development. The fifth facet of outcomes focuses on the yields of SSCM’s pursuit.Originality/valueThese principal facets are built across the multiple levels and unique conceptual standpoints as propagated by 13 themes and 34 sub-themes. These themes are generated based upon 419 articles (2000-2017) from more than 40 leading journals. The authors discuss the facet-specific key implications for guiding the literature in its further advancement, and thus propose a rigorous thematic landscape of the SSCM literature with a unique approach. Overall, the outcomes of this review provide a fundamental organization of the SSCM literature – from the perspective of a journey involved in the transition from traditional to sustainable supply chains.
Is independence really an opportunity? The experience of entrepreneur-mothers
Authors: Foley Meraiah , Baird Marian , Cooper Rae , Williamson Sue 
Published: February 2018

PurposeThe purpose of this paper is to explore how entrepreneur-mothers experience independence in the transition to entrepreneurship, and whether they perceive independence as an agentic, opportunity-maximisation motive or a constrained, necessity-driven response.Design/methodology/approachAdopting a qualitative and interpretive approach, the authors analysed interviews with 60 entrepreneur-mothers to refine conceptual understanding of independence.FindingsThe authors find that entrepreneur-mothers experience independence not as an opportunity, but as a functional necessity in managing the temporal and perceived moral demands of motherhood. The authors assert that there is a fundamental difference between wanting independence to pursue a more autonomous lifestyle, and needing independence to attend to family obligations, a difference that is not adequately captured in the existing conceptualisation of independence. Consequently, the authors propose the classification of “family-driven entrepreneurship” to capture the social and institutional factors that may disproportionately push women with caregiving responsibilities towards self-employment.Practical implicationsThis paper proposes that a new category of entrepreneurial motivation be recognised to better account for the social and institutional factors affecting women’s entrepreneurship, enabling policymakers to more accurately position and support entrepreneur-mothers.Social implicationsThe authors challenge the existing framing of independence as an agentic opportunity-seeking motive, and seek to incorporate family dynamics into existing entrepreneurial models.Originality/valueThis paper delivers much-needed conceptual refinement of independence as a motivator to entrepreneurship by examining the experiences of entrepreneur-mothers, and proposes a new motivational classification, that of family-driven entrepreneurship to capture the elements of agency and constraint embedded in this transition.
A sensitive flower: mechanical stimulation induces rapid flower closure in Drosera spp. (Droseraceae)
Authors: Tagawa Kazuki , Watanabe Mikio , Yahara Tetsukazu 
Published: February 2018

AbstractSome plants rapidly close their leaves in response to mechanical stimulation, but no case is known in which mechanical stimulation causes rapid petal closure. In this study, we found that Drosera tokaiensis closes petals within 2–10 min after experimental stimulation of the calyx, closed flowers or scapes with a pair of tweezers. Although petal closure was induced more rapidly by touching a position closer to a flower, it was not induced by stimulating stamens and pistils. The habit of petal closure varies among species of Drosera: after experimental stimulations of the calyx or scapes, D. tokaiensis and D. spatulata often closed petals but D. rotundifolia and D. toyoakensis did not close them. The petal closure may function as defense against a specialist florivore.
VPS9 domain‐containing proteins are essential for autophagy and endocytosis in Pyricularia oryzae
Authors: Zhu Xue‐Ming , Liang Shuang , Shi Huan‐Bin , Lu Jian‐Ping , Dong Bo , Liao Qian‐Sheng , Lin Fu‐Cheng , Liu Xiao‐Hong 
Published: February 2018

SummaryPyricularia oryzae is the causal pathogen of rice blast disease. Autophagy has been shown to play important roles in P. oryzae development and plant infection. The P. oryzae endosomal system is highly dynamic and has been shown to be associated with conidiogenesis and pathogenicity as well. To date, the crosstalk between autophagy and endocytosis has not been explored in P. oryzae. Here, we identified three P. oryzae VPS9 domain‐containing proteins, PoVps9, PoMuk1 and PoVrl1. We found that PoVps9 and PoMuk1 are localized to vesicles and are each co‐localized with PoVps21, a recognized marker of early endosomes. Deletion of PoVPS9 resulted in severe defects in endocytosis and autophagosome degradation and impaired the localization of PoVps21 to endosomes. Additionally, deletion of the PoMUK1 gene in the ΔPovps9 mutant background exhibited more severe defects in development, autophagy and endocytosis compared with the ΔPovps9 mutant. Pull‐down assay showed that PoVps9 interacts with PoVps21, PoRab11 and PoRab1, which have been verified to participate in endocytosis. Furthermore, yeast two‐hybrid and co‐immunoprecipitation assays confirmed that PoVps9 directly interacts with the GDP form of PoVps21. Thus, PoVps9 is a key protein involved in autophagy and in endocytosis.
Responses to warnings about the impact of eating disorders on fertility: a qualitative study
Authors: Holmes Su 
Published: February 2018

AbstractEating disorders (EDs) have often been discussed as a risk to reproductive health. But existing research is quantitative in nature, paying no attention to issues of patient experience. In discussing data from 24 semi‐structured interviews, this article draws on sociological approaches to medical ‘risk’ and feminist approaches toEDs to explore how women with experience of anEDresponded to fertility warnings within treatment contexts. In doing so, it is suggested that responses to fertility warnings offer unique insight into the potentially damaging limitations of biomedical approaches to eating problems and their focus onEDs as individual ‘pathologies’ (rather than culturally embedded expressions of gendered embodiment). At best warnings are seen as making problematic assumptions about the aspirations of female patients, which may curtail feelings of agency and choice. At worst, they may push women further into destructive bodily and eating practices, and silence the distress that may be articulated by anED.
The career aspirations and expectations of geography doctoral students: establishing academic subjectivities within a shifting landscape
Authors: Dufty‐Jones Rae 
Published: February 2018

AbstractThe PhD forms a watershed period where candidates' professional identities are formed, and their career aspirations and expectations are developed. Yet little is known about Australian geography doctoral students' career aspirations and expectations. Drawing on findings from a 2016 survey of those students, the paper establishes that while a majority of students aspire to work in academia, many also feel quite pessimistic about their prospects of being able to do so. The paper argues that the uncertainty and anxiety that many Australian geography doctoral students feel about their future careers is the product of a wider cultural shift that is occurring in relation to the purpose of the research doctorate. Geographers need to critically engage with these changes and the academic subjectivities they are producing to ensure that, as a discipline, geography continues to make a positive contribution to the careers of the individuals who undertake these degrees.
Input data needed for a risk model for the entry, establishment and spread of a pathogen (Phomopsis vaccinii) of blueberries and cranberries in the EU
Authors: van Bruggen A.H.C. , West J.S. , van der Werf W. , Potting R.P.J. , Gardi C. , Koufakis I. , Zelenev V.V. , Narouei‐Khandan H. , Schilder A. , Harmon P. 
Published: February 2018

AbstractInternational trade in live plant materials has increased worldwide over the last four decades. This has led to a dramatic increase in the introduction, establishment and spread of non‐native plant pathogens. Regulatory authorities need advice on measures that may mitigate these adverse consequences of trade. Risk models may be used to underpin such advice. In this review, we give a systematic overview of the data needed for a quantitative risk model for Phomopsis vaccinii, which causes stem and fruit infections on Vaccinium species, and sometimes death, potentially also on native wild Vaccinium species in the EU. P. vaccinii is a quarantine organism worldwide, except for North America, where it is endemic. Despite extensive knowledge of the aetiology of the diseases caused by this pathogen and its taxonomy, quantitative data on transportation and detection of infected plants for planting and berries are scarce, and quantitative assessment of the future introduction, establishment and spread of P. vaccinii is difficult. Estimation of the potential impact of this pathogen in production fields and wild Vaccinium stands is even more difficult. P. vaccinii is not unique in this respect, and this review indicates the need for more and better quantitative data for assessment of the risks posed by newly introduced plant pathogens in areas where they are not endemic.
How blind are they? Phototactic responses in stygobiont diving beetles (Coleoptera: Dytiscidae) from calcrete aquifers of Western Australia
Authors: Langille Barbara L , Tierney Simon M , Austin Andrew D , Humphreys William F , Cooper Steven J B 
Published: February 2018

AbstractSubterranean water beetles endemic to groundwater calcretes of Western Australia exhibit convergent traits typical of troglomorphic arthropods, including loss of eyes, pigmentation and wings. As these dytiscid species are estimated to have been isolated underground in permanent darkness for over three million years, it is predicted that they will completely lack phototactic responses. We tested this hypothesis by analysing the behaviour of six subterranean beetle species within an observational arena with dark and light hemispheres. Scan samples at 1 min intervals and total time spent on each hemisphere were recorded over a 20 min period, testing at least 15 individuals per species. We quantified behaviour as an index (dark ratio) so that individual species in this, and future, studies can be consistently compared. Results analysed as both categorical and absolute proportion of time spent in each hemisphere suggest negative phototaxis in Paroster macrosturtensis. The remaining five species did not display any preference for either light or dark hemispheres. These results raise the possibility that some ancestral Paroster species may have exhibited negative phototactic behaviour prior to subterranean colonization. The retention of such a behavioural trait in lightless environments could represent the maintenance for some unknown pleiotropic function. Alternatively, it is possible that insufficient time has passed for neutral processes to render photoreception genes and phototactic behaviours non‐functional. Our study adds to a growing body of evidence that implies highly troglomorphic animals may have evolved from ancestral species that exhibited negative phototaxis as a preadaptation to living in permanent darkness.
“All There Is”: The Reconciliatory Poetics of a Singing Voice
Authors: Taylor‐Neu Robyn 
Published: February 2018

ABSTRACTIn this article, I argue that popular representations of Tanya Tagaq, an Indigenous Canadian musician, shore up a reconciliatory project by mobilizing voice ideologies within densely poetic texts. I trace how commonly held presuppositions and fantasies about Indigeneity in the Canadian multicultural context emerge through and are reinforced by formal aspects of music‐critical discourse. By analyzing how discourse on cultural production figurates reconciliation at various scales, this article contributes to critical understandings of liberal multicultural politics of reconciliation. In so doing, it offers insight into an entanglement of voice, language, and media ideologies in popular journalistic discourse on cultural difference. [voice, reconciliation, semiotics, Indigeneity, Canada]
Clinical safety, tolerability, pharmacokinetics and effects on urinary electrolyte excretion of AZD9977, a novel, selective mineralocorticoid receptor modulator
Authors: Erlandsson Fredrik , Albayaty Muna , Chialda Ligia , Ericsson Hans , Amilon Carl , Nelander Karin , Jansson‐Löfmark Rasmus , Wernevik Linda , Kjaer Magnus , Bamberg Krister , Hartleib‐Geschwindner Judith 
Published: February 2018

AimsAZD9977 is the first mineralocorticoid receptor modulator in clinical development exerting similar organ protection as eplerenone with minimal urinary electrolyte effects in preclinical studies. The aim was to perform the initial clinical assessment of AZD9977.MethodsA first‐in‐human trial explored doses from 5 to 1200 mg. To study effects on urinary electrolyte excretion an additional randomized placebo controlled cross‐over four‐period clinical trial was performed. Twenty‐three healthy volunteers were administered fludrocortisone alone or in combination with AZD9977, eplerenone or both. AZD9977/eplerenone combination was given to assess if AZD9977 can attenuate eplerenone induced natriuresis.ResultsAZD9977 at doses from 5 to 1200 mg was safe and well tolerated and pharmacokinetics were compatible with further development. AZD9977 exhibited similar effects on urinary ln [Na+]/[K+] as eplerenone when using fludrocortisone as mineralocorticoid receptor agonist, and the combination had an additive effect on ln [Na+K+].ConclusionsThe results in man contradict the results in rodent models driven by aldosterone, in which AZD9977 has minimal electrolyte effects. Future clinical studies with AZD9977 should be performed in presence of endogenous or exogenous aldosterone to assess potential benefit of AZD9977 in patients.
Response and survival for primary therapy combination regimens and maintenance rituximab in Waldenström macroglobulinaemia
Authors: Castillo Jorge J. , Gustine Joshua N. , Meid Kirsten , Dubeau Toni E. , Severns Patricia , Xu Lian , Yang Guang , Hunter Zachary R. , Treon Steven P. 
Published: February 2018

SummaryWaldenström macroglobulinaemia (WM) is a rare and incurable lymphoma. Comparative studies evaluating the efficacy of primary therapy in symptomatic WM patients have not been performed. In this study, we compared response and survival outcomes in WM patients who received primary therapy with cyclophosphamide‐dexamethasone‐rituximab (CDR), bortezomib‐dexamethasone‐rituximab (BDR) and bendamustine‐rituximab (Benda‐R), as well as maintenance rituximab following primary therapy. Analyses were adjusted for relevant clinical factors associated with response and survival. Maintenance rituximab was analysed as a time‐varying covariate. Our study included 182 patients, of which 57 (31%) received Benda‐R, 87 (48%) BDR and 38 (21%) CDR; 116 (64%) received maintenance rituximab. The median time to best response was shorter for Benda‐R and BDR than CDR (18, 20 and 30 months, respectively). Benda‐R and BDR were associated with better median progression‐free survival (PFS) than CDR (5·5, 5·8 and 4·8 years, respectively), and better 10‐year overall survival rates (OS; 95%, 96% and 81%, respectively). Maintenance rituximab was associated with higher rates of major response (97% vs. 68%), and better median PFS (6·8 years vs. 2·8 years) and 10‐year OS rate (84% vs. 66%) when compared to not receiving maintenance. Benda‐R, BDR and maintenance rituximab associate with higher response rates and longer survival in WM patients than CDR and no maintenance, respectively.
The diagnostic value of cell blocks in Liquid‐Based Cytology of bronchial aspiration and bronchial brushing materials
Authors: Sadullahoğlu C. , Yaman B. , Veral A. , Nart D. 
Published: February 2018

IntroductionFibreoptic bronchoscopy is used for the diagnosis and treatment of several pulmonary diseases. Conventional smear and Liquid‐based cytology (LBC) methods are applied to cytology samples of various bronchoscopic techniques. If the cytology sample is sufficient for evaluation, a cell block (CB) can be prepared from the remaining material. The aim of this study is to identify the diagnostic value of conventional smear, LBC and CB methods in bronchial cytological specimens.MethodsA retrospective review of 329 samples from 240 patients was made and, of these, 144 patients were found to have neoplasia. A blind review of the specimens was performed and all were reclassified individually. The endoscopic findings of the 144 patients with neoplasia were analysed retrospectively. The cytological diagnoses were then compared with the final diagnosis or the endoscopic findings of patients with neoplasia. The sensitivity was calculated for each method, both separately and together.ResultsIt was determined that CB led to a 10.1% increase in the diagnostic sensitivity for bronchial aspiration (BA) specimens, while no significant increase was seen in bronchial brush specimens. In BA specimens of neoplasia patients with normal bronchoscopic findings, while three methods were applied together with an increase in the number of cases diagnosed as malignant cytology, there was no significant increase in bronchial brush specimens.ConclusionThis study demonstrated that adding cell block to CB and LBC seemed to contribute the cytological diagnosis in BA materials significantly. Another advantage of CB is the opportunity of applying advanced methods such as immunocytochemical and molecular techniques.
Pathogen spillover during land conversion
Authors: Faust Christina L. , McCallum Hamish I. , Bloomfield Laura S. P. , Gottdenker Nicole L. , Gillespie Thomas R. , Torney Colin J. , Dobson Andrew P. , Plowright Raina K. 
Published: February 2018

AbstractPathogen spillover from wildlife to domestic animals and humans, and the reverse, has caused significant epidemics and pandemics worldwide. Although pathogen emergence has been linked to anthropogenic land conversion, a general framework to disentangle underlying processes is lacking. We develop a multi‐host model for pathogen transmission between species inhabiting intact and converted habitat. Interspecies contacts and host populations vary with the proportion of land converted; enabling us to quantify infection risk across a changing landscape. In a range of scenarios, the highest spillover risk occurs at intermediate levels of habitat loss, whereas the largest, but rarest, epidemics occur at extremes of land conversion. This framework provides insights into the mechanisms driving disease emergence and spillover during land conversion. The finding that the risk of spillover is highest at intermediate levels of habitat loss provides important guidance for conservation and public health policy.