bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengaruh Pasca-Pemrosesan Annealing Terhadap Sifat Mekanis Polimer Hasil Cetak 3D Fused Deposition Modeling (FDM)
Authors: Pratama Juan 
Published: November 2024

Nowadays, 3-dimensional (3D) printing technology using the Fused Deposition Modeling (FDM) type for processing polymer materials is widely known and used because of its capability to produce products with complex shapes. Even though they have many advantages, FDM 3D printed products also have several disadvantages, where low mechanical properties are one of the main weaknesses of FDM 3D printed products caused by the non-uniform cooling process in the FDM 3D printed polymer material. In this research, post-processing annealing was carried out to determine the effect of reheating and uniform cooling on the mechanical properties of polylactic acid (PLA) 3D printed FDM results. The specimens were printed by varying the raster angle in the print parameters. Then, 3 variations of annealing temperature were used, i.e., 80, 120, and 150 °C. The research results show that post-processing annealing does not have a significant effect on the tensile strength, elongation, and elastic modulus of 3D FDM printed PLA, regardless of the raster angle used. In the end, this research can confirm one of the previous findings that post-processing annealing is a less effective method for improving the mechanical properties of FDM 3D printed products, especially PLA.
ANALISIS SENTIMEN KOMENTAR INSTAGRAM TERHADAP WACANA KEBIJAKAN ELECTRONIC ROAD PRICING (ERP) DI JAKARTA MENGGUNAKAN ALGORITMA NAÏVE BAYES
Authors: Burhansyah Farhan Nugroho , Saputra Yan Sofyan Andhana 
Published: November 2024

Pemerintah DKI memiliki rencana mengendalikan lalu lintas dengan memanfaatkan Jalan Berbayar Elektronik atau Electronic Road Pricing. Untuk mengetahui respon pengguna media sosial terhadap kebijakan tersebut, dilakukan analisis sentimen pada komentar aplikasi Instagram. Algoritma yang digunakan pada analisis sentimen ini adalah algoritma Naïve Bayes. Rancangan penelitian berdasarkan metodologi Knowledge Discovery in Databases, disingkat KDD. Penelitian ini menggunakan web scraping Apify untuk mengambil data postingan selama tahun 2023 sebanyak 1486 komentar aplikasi Instagram, dan python sebagai bahasa pemrogramannya. Penelitian ini menghasilkan kesimpulan kedalam tiga kelas sentimen, dengan jumlah masing-masing 97 label positif, 568 label negatif, dan 743 label netral. Untuk mengukur tingkat akurasi algoritma, pelaksanaan pengujian menggunakan metode split data 4 model, berturut-turut adalah model 90:10, 80:20, 70:30, dan 60:40. Pengujian akurasi dengan confusion matrix menunjukkan nilai akurasi tertinggi terdapat pada model 90:10, sedangkan pengujian akurasi dengan grafik ROC menunjukkan nilai AUC tertinggi pada model 90:20 dengan nilai 0.7335, sehingga bisa disimpulkan model berkualitas baik dalam membedakan kelas.
Hubungan Pengetahuan dan Sikap Lanjut Usia Tentang Diet Rendah Purin Terhadap Pencegahan Asam Urat Di Desa Matiti Kecamatan Doloksanggul Kabupaten Humbang Hasundutan Tahun 2023
Authors: Pakpahan Camelia , Simamora Rouli DF 
Published: November 2024

Latar Belakang : Asam urat adalah produk akhir dari pemecahan purin. Sekitar 85% asam urat dapat diproduksi sendiri oleh tubuh melalui nukleotida purin endogen, Guanic Acid (GMP), Insonic Acid (IMP), Adenic Acid (AMP). Pada pria asam urat norma yaitu 3,0-7,0mg/dl dan untuk wanita yaitu 2,4-6,0/dl Tujuan penelitian ini adalah untuk mengetahui Hubungan Pengetahuan dan Sikap Lanjut Usia terhadap Pencegahan Asam Urat di Desa Matiti I Kecamatan Doloksanggul Kabupaten Humbang Hasundutan Tahun 2023.Tujuan: untuk mengetahui hubungan dukungan keluarga terhadap kemandirian lansia usia 65-74 tahun dalam pemenuhan aktivitas lansia sehari hari di Desa Matiti I Kecamatan Doloksanggul kabupaten Humbang Hasundutann tahun 2023. Metode : penelitian deskriptif analitik yang dilakukan dengan desain cross sectional. Populasi sebanyak 120 orang dan pengambilan sampel dengan penggunaan rumus Slovin dengan jumlah sampel 54 orang. Pengumpulan data menggunakan kuesioner, kemudian diolah dengan uji chi-square. Hasil : bungan terhadap pemenuhan aktivitas sehari-hari dengan menggunakan uji chi square dengan tingkat kepercayaan 90% dan dan df=1, diperoleh X² hitung 3,264> X² tabel 2,705, dan variabel kemandirian memiliki hubungan terhadap pemenuhan aktivitas sehari-hari dengan menggunakan uji chi-square dengan tingkat kepercayaan 90% dan df=1, diperoleh X² hitung 3,351> X² tabel 2,705. Diskusi : Diharapkan bagi setiap keluarga yang memiliki lansia berada Di Desa Matiti I Kecamatan Doloksanggul Kabupaten Humbang Hasundutan agar meningkatkan dukungan keluarga terhadap kemandirian lansia dalam melakukan aktivitas sehari- hari.
Effect of Organic Fertilization Based on Cattle Dung in Village Cultivation of Sugar Cane (Saccharum officinarum L.) in Immature Phase in Northern Ivory Coast
Authors: Joseph Allé Yamoussou , Ibrahim Sylla , Sahabane Traoré Mohamed , Gnenakan Yéo , Lassina Fondio 
Published: November 2024

This study aimed to determine the dose of organic fertilizer adapted to the agroecological conditions of the Ferkéssédougou zone during the immature or vegetative phase with a view to improving agricultural productivity in rainfed sugar cane cultivation. The trial was conducted using a Fisher block experimental design with three (3) repetitions and six (6) treatments including the control treatment. The observations mainly focused on growth parameters. Health monitoring was also carried out to detect the presence of diseases. Regarding growth parameters, no statically significant effect of fertilizers was observed between treatments. However, the fertilizers used had a positive impact in terms of gain compared to the control. Thus, three (3) groups of fertilizers stood out. Group 1 was composed of mineral manure at the normal dose (350 kg/ha of NPK), organic manure at the normal dose (20t/ha), double dose of compost (40 t/ha) and the organo-mineral mixture, namely the normal dose of compost (20t/ha) + half dose of mineral fertilizer (175 kg/ha of NPK). These treatments promoted good emergence, good elongation of the plants with large diameters and a large number of internodes. Group 2, composed only of half a dose of compost (10 t/ha), allowed us to have a large number of stems. For group 3, corresponding to the control treatment, no growth parameter was correlated. No symptoms of major illnesses were observed during the observations. The M1400/86 variety used is resistant to termite attack.
CPP‐E1A fusion peptides inhibit CtBP‐mediated transcriptional repression
Authors: Blevins Melanie A. , Zhang Caiguo , Zhang Lingdi , Li Hong , Li Xueni , Norris David A. , Huang Mingxia , Zhao Rui 
Published: June 2018

The carboxyl‐terminal binding proteins (CtBP) are transcriptional corepressors that regulate the expression of multiple epithelial‐specific and pro‐apoptotic genes. Overexpression of CtBP occurs in many human cancers where they promote the epithelial‐to‐mesenchymal transition, stem cell‐like features, and cell survival, while knockdown of CtBP in tumor cells results in p53‐independent apoptosis. CtBPs are recruited to their target genes by binding to a conserved PXDLS peptide motif present in multiple DNA‐binding transcription factors. Disrupting the interaction between CtBP and its transcription factor partners may be a means of altering CtBP‐mediated transcriptional repression and a potential approach for cancer therapies. However, small molecules targeting protein–protein interactions have traditionally been difficult to identify. In this study, we took advantage of the fact that CtBP binds to a conserved peptide motif to explore the feasibility of using peptides containing the PXDLS motif fused to cell‐penetrating peptides (CPP) to inhibit CtBP function. We demonstrate that these peptides disrupt the ability of CtBP to interact with its protein partner, E1A, in an AlphaScreen assay. Moreover, these peptides can enter both lung carcinoma and melanoma cells, disrupt the interaction between CtBP and a transcription factor partner, and inhibit CtBP‐mediated transcriptional repression. Finally, the constitutive expression of one such peptide, Pep1‐E1A‐WT, in a melanoma cell line reverses CtBP‐mediated oncogenic phenotypes including proliferation, migration, and sphere formation and limits tumor growth in vivo. Together, our results suggest that CPP‐fused PXDLS‐containing peptides can potentially be developed into a research tool or therapeutic agent targeting CtBP‐mediated transcriptional events in various biological pathways.
Experimente mit Hefe
Authors: Sommer Isabell 
Published: June 2018

ZusammenfassungHefe eignet sich hervorragend für einfache Experimente zur Visualisierung von Stoffwechselvorgängen, wie man sie beispielsweise vom Brotbacken kennt. So kann die Verstoffwechselung von verschiedenen Zuckern zu Kohlenstoffdioxid qualitativ über das Aufblähen von Luftballons bzw. quantiativ über die Gasproduktion in einem Gärröhrchen anschaulich gezeigt werden. Die vorgestellten Experimente können dazu genutzt werden, die Frage zu klären, warum Hefe einem Brotteig zugesetzt wird, obwohl sie nicht in der Lage ist, die im Mehl vorhandene Stärke umzusetzen.
TGF‐β–responsive CAR‐T cells promote anti‐tumor immune function
Authors: Hou Andrew J. , Chang ZeNan L. , Lorenzini Michael H. , Zah Eugenia , Chen Yvonne Y. 
Published: June 2018

AbstractA chimeric antigen receptor (CAR) that responds to transforming growth factor beta (TGF‐β) enables the engineering of T cells that convert this immunosuppressive cytokine into a potent T‐cell stimulant. However, clinical translation of TGF‐β CAR‐T cells for cancer therapy requires the ability to productively combine TGF‐β responsiveness with tumor‐targeting specificity. Furthermore, the potential concern that contaminating, TGF‐β?producing regulatory T (Treg) cells may preferentially expand during TGF‐β CAR‐T cell manufacturing and suppress effector T (Teff) cells demands careful evaluation. Here, we demonstrate that TGF‐β CAR‐T cells significantly improve the anti‐tumor efficacy of neighboring cytotoxic T cells. Furthermore, the introduction of TGF‐β CARs into mixed T‐cell populations does not result in the preferential expansion of Treg cells, nor do TGF‐β CAR‐Treg cells cause CAR‐mediated suppression of Teff cells. These results support the utility of incorporating TGF‐β CARs in the development of adoptive T‐cell therapy for cancer.
Personal background, motivation and interpersonal style of psychotherapy trainees having different theoretical orientations: An Italian contribution to an international collaborative study on psychotherapy training
Authors: Messina Irene , Gelo Omar C.G. , Gullo Salvo , Sambin Marco , Mosconi Andrea , Fenelli Antonio , Curto Marcello , Orlinsky David 
Published: June 2018

AbstractBackgroundTherapist characteristics seem to be a key factor in clinical effectiveness. Trainees’ personal and professional background, motivation, and interpersonal style have been shown to deserve attention in previous research concerning therapist development and warrant further study due to their potential implications for psychotherapy training outcomes.AimTo explore personal and professional background, motivation, and interpersonal style of psychotherapy trainees with different theoretical approaches.FindingsIn the present study, 135 post‐graduate psychotherapy trainees with different theoretical approaches (psychodynamic, systemic, and cognitive‐behavioral) completed the Trainee Background Information Form (TBIF), reporting information concerning their background, style of relating, and motivations. Personal experiences of trainees were frequently reported as a motivation in undertaking psychotherapy training and were associated with negative experiences in childhood. Significant differences were found in the motivations and interpersonal style of trainees with different theoretical approach.ImplicationsPersonal experiences, motivation and interpersonal styles should be taken into consideration in future studies on therapists’ development and in the implementation of psychotherapy programs.
Indolent T‐cell‐rich small B‐cell hepatic lymphoma in a Golden Retriever
Authors: Rout Emily D. , Hughes Kelly L. , Boostrom Brendan O. , Seelig Davis M. , Avery Anne C. , Avery Paul R. 
Published: June 2018

Key Clinical MessageAn 11‐year‐old female spayed Golden Retriever presented for an incidentally found liver mass. The hepatic mass and intra‐abdominal lymph nodes had a marked heterogeneous T‐cell population and far fewer numbers of small clonal B cells. This T‐cell‐rich small B‐cell lymphoma had a unique histological pattern and indolent clinical course.
Proof of Equivalent Catalytic Functionality upon Photon‐Induced and Thermal Activation of Supported Isolated Vanadia Species in Methanol Oxidation
Authors: Kortewille Bianca , Wachs Israel E. , Cibura Niklas , Pfingsten Oliver , Bacher Gerd , Muhler Martin , Strunk Jennifer 
Published: June 2018

AbstractThe front cover artwork for issue 11/2018 results from a collaboration between the Leibniz Institute for Catalysis (Germany), Lehigh University (PA/USA), the Max‐Planck‐Institute for Chemical Energy Conversion, the University Duisburg‐Essen and the Ruhr‐University Bochum (all in Germany). The image shows an isolated vanadium oxide species supported on silica which performs selective oxidation of methanol both photon‐induced and by thermal activation. See the Communication itself at https://doi.org/10.1002/cctc.201800311.
A clinical and proteomics approach to predict the presence of obstructive peripheral arterial disease: From the Catheter Sampled Blood Archive in Cardiovascular Diseases (CASABLANCA) Study
Authors: McCarthy Cian P. , Ibrahim Nasrien E. , van Kimmenade Roland R.J. , Gaggin Hanna K. , Simon Mandy L. , Gandhi Parul , Kelly Noreen , Motiwala Shweta R. , Mukai Renata , Magaret Craig A. , Barnes Grady , Rhyne Rhonda F. , Garasic Joseph M. , Januzzi James L. 
Published: June 2018

BackgroundPeripheral arterial disease (PAD) is a global health problem that is frequently underdiagnosed and undertreated. Noninvasive tools to predict the presence and severity of PAD have limitations including inaccuracy, cost, or need for intravenous contrast and ionizing radiation.HypothesisA clinical/biomarker score may offer an attractive alternative diagnostic method for PAD.MethodsIn a prospective cohort of 354 patients referred for diagnostic peripheral and/or coronary angiography, predictors of ≥50% stenosis in ≥1 peripheral vessel (carotid/subclavian, renal, or lower extremity arteries) were identified from >50 clinical variables and 109 biomarkers. Machine learning identified variables predictive of obstructive PAD; a score derived from the final model was developed.ResultsThe score consisted of 1 clinical variable (history of hypertension) and 6 biomarkers (midkine, kidney injury molecule‐1, interleukin‐23, follicle‐stimulating hormone, angiopoietin‐1, and eotaxin‐1). The model had an in‐sample area under the receiver operating characteristic curve of 0.85 for obstructive PAD and a cross‐validated area under the curve of 0.84; higher scores were associated with greater severity of angiographic stenosis. At optimal cutoff, the score had 65% sensitivity, 88% specificity, 76% positive predictive value (PPV), and 81% negative predictive value (NPV) for obstructive PAD and performed consistently across vascular territories. Partitioning the score into 5 levels resulted in a PPV of 86% and NPV of 98% in the highest and lowest levels, respectively. Elevated score was associated with shorter time to revascularization during 4.3 years of follow‐up.ConclusionsA clinical/biomarker score demonstrates high accuracy for predicting the presence of PAD.
Comparison of new element designs for combined RF‐shim arrays at 7 T
Authors: Winkler Simone A. , Warr Paul A. , Stockmann Jason P. , Mareyam Azma , Keil Boris , Watkins Ronald D. , Wald Lawrence L. , Rutt Brian K. 
Published: June 2018

AbstractIn this work we identify novel concepts for RF‐shim loop architectures suitable for 7 T made of 2 concentric conducting loops fulfilling RF and DC functions, respectively, and to determine their relative SNR performance. The goal is to minimize interference between the 2 systems while making efficient use of the space closest to the body. We show by means of theoretical derivation of the frequency spectrum that the proposed 2‐loop structure exhibits an anti‐resonant null and a resonant peak in the frequency domain. The proposed structure is comprised of 2 concentric wire loops either arranged as nested loops or in the form of a coaxial cable, in which the 2 conductors carry the RF and shim signals, respectively. We use theory, simulation, and phantom measurements to obtain frequency spectra and SNR maps for the proposed structures. Retained SNR is found to be 75% in the coaxial loop and ranges from 57% to 67% in 3 different coaxial configurations. We have found both implementations to be a viable concept for the use in RF‐shim devices if remaining SNR limitations can be overcome. We have investigated 2 new design modalities in 7 T RF‐shim coil design that separate the RF and shim conductors such that the previously proposed toroidal chokes are eliminated—thereby removing undesired additional loss, bulk, and design complexity.
Generating FN3‐Based Affinity Reagents Through Phage Display
Authors: Gorman Kevin , McGinnis Jennifer , Kay Brian 
Published: June 2018

AbstractAntibodies are useful tools for detecting individual proteins in complex samples and for learning about their location, amount, binding partners, and function in cells. Unfortunately, generating antibodies is time consuming and laborious, and their affinity and/or specificity is often limited. This protocol offers a fast and inexpensive alternative to generate antibody surrogates through phage display of a library of fibronectin type III (FN3) monobody variants and affinity selection for binders. © 2018 by John Wiley & Sons, Inc.
Non‐Steric Interactions Predict the Trend and Steric Interactions the Offset of Protein Stability in Cells
Authors: Davis Caitlin M. , Gruebele Martin 
Published: June 2018

AbstractAlthough biomolecules evolved to function in the cell, most biochemical assays are carried out in vitro. In‐cell studies highlight how steric and non‐steric interactions modulate protein folding and interactions. VlsE and PGK present two extremes of chemical behavior in the cell: the extracellular protein VlsE is destabilized in eukaryotic cells, whereas the cytoplasmic protein PGK is stabilized. VlsE and PGK are benchmarks in a systematic series of solvation environments to distinguish contributions from non‐steric and steric interactions to protein stability, compactness, and folding rate by comparing cell lysate, a crowding agent, ionic buffer and lysate buffer with in‐cell results. As anticipated, crowding stabilizes proteins, causes compaction, and can speed folding. Protein flexibility determines its sensitivity to steric interactions or crowding. Non‐steric interactions alone predict in‐cell stability trends, while crowding provides an offset towards greater stabilization. We suggest that a simple combination of lysis buffer and Ficoll is an effective new in vitro mimic of the intracellular environment on protein folding and stability.
Recording and Analyzing Nucleic Acid Distance Distributions with X‐Ray Scattering Interferometry (XSI)
Authors: Zettl Thomas , Das Rhiju , Harbury Pehr A. B. , Herschlag Daniel , Lipfert Jan , Mathew Rebecca S. , Shi Xuesong 
Published: June 2018

AbstractMost structural techniques provide averaged information or information about a single predominant conformational state. However, biological macromolecules typically function through series of conformations. Therefore, a complete understanding of macromolecular structures requires knowledge of the ensembles that represent probabilities on a conformational free energy landscape. Here we describe an emerging approach, X‐ray scattering interferometry (XSI), a method that provides instantaneous distance distributions for molecules in solution. XSI uses gold nanocrystal labels site‐specifically attached to a macromolecule and measures the scattering interference from pairs of heavy metal labels. The recorded signal can directly be transformed into a distance distribution between the two probes. We describe the underlying concepts, present a detailed protocol for preparing samples and recording XSI data, and provide a custom‐written graphical user interface to facilitate XSI data analysis. © 2018 by John Wiley & Sons, Inc.
Rational Design of Low Cost and High Energy Lithium Batteries through Tailored Fluorine‐free Electrolyte and Nanostructured S/C Composite
Authors: Agostini M. , Lim D.‐H. , Sadd M. , Hwang J.‐Y. , Brutti S. , Heo J. W. , Ahn J. H. , Sun Y. K. , Matic A. 
Published: June 2018

AbstractWe report a new Li–S cell concept based on an optimized F‐free catholyte solution and a high loading nanostructured C/S composite cathode. The Li2S8 present in the electrolyte ensures both buffering against active material dissolution and Li+ conduction. The high S loading is obtained by confining elemental S (≈80 %) in the pores of a highly ordered mesopores carbon (CMK3). With this concept we demonstrate stabilization of a high energy density and excellent cycling performance over 500 cycles. This Li–S cell has a specific capacity that reaches over 1000 mA h g−1, with an overall S loading of 3.6 mg cm−2 and low electrolyte volume (i.e., 10 μL cm−2), resulting in a practical energy density of 365 Wh kg−1. The Li–S system proposed thus meets the requirements for large scale energy storage systems and is expected to be environmentally friendly and have lower cost compared with the commercial Li‐ion battery thanks to the removal of both Co and F from the overall formulation.
Structural optimization of silicon for high efficiencies of light emission
Authors: Sugishita Yuma , Inukai Keisuke , Goshima Keishiro 
Published: June 2018

AbstractConventional metal patterns in integrated circuits are known to exhibit drawbacks such as signal delays and heat generation. The use of the silicon photonics technology has been proposed to overcome these drawbacks. Silicon‐based materials and processing technologies offer cost‐effective solutions for large‐scale fabrication in microelectronics, such as for the fabrication of silicon integrated circuits. However, the fabrication of light‐emitting diodes using silicon‐based materials for the silicon photonics has not been realized so far. We investigated the structural optimization of silicon to obtain a high efficiency of light emission. And we observed the correlation between the high‐efficiency light emission and alkali impurities such as Na and K.