bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantifying neurobehavioral profiles across neurodevelopmental genetic syndromes and idiopathic neurodevelopmental disorders
Authors: Frazier Thomas W. , Busch Robyn M. , Klaas Patricia , Lachlan Katherine , Loth Eva , Smith‐Hicks Constance , Sahin Mustafa , Hardan Antonio Y. , Uljarevic Mirko ,   NET Development Project Team
Published: November 2024

AbstractAimTo examine neurobehavioral findings in three genetic syndromes (PTEN hamartoma tumor syndrome, Malan syndrome [mutations in the NFIX gene], and SYNGAP1‐related disorder), a mixed group of other neurodevelopmental genetic syndromes (NDGS), idiopathic neurodevelopmental disorder, and neurotypical control participants.MethodUsing a longitudinal case–control design, caregivers reported neurobehavioral information for 498 participants (PTEN hamartoma tumor syndrome n = 112, Malan syndrome n = 24, SYNGAP1‐related disorder n = 47, other NDGS n = 72, idiopathic neurodevelopmental disorder n = 54, neurotypical siblings n = 74, and unrelated neurotypical control participants n = 115) at three timepoints (baseline, and 1‐month and 4‐month follow‐ups) using the online‐administered Neurobehavioral Evaluation Tool (NET).ResultsNET scales had good scale and test–retest reliability. Unique patterns of neurobehavioral findings emerged, with SYNGAP1‐related disorder and Malan syndrome showing generally more severe symptom and skill patterns than for other groups of patients. Patterns could be partly accounted for by estimated cognitive level, speech level, and the presence of autism spectrum disorder. However, even when accounting for these factors, group differences remained. Reliable change indices are reported.InterpretationGenetic syndromes associated with neurodevelopmental disorders present with unique neurobehavioral profiles that can inform selection of outcome measures in future clinical trials. The NET may be a useful screening and monitoring instrument in clinical practice, where frequent in‐person clinic attendance is difficult for many patients.
Female Wistar Kyoto More Immobile rats with genetic stress hyper‐reactivity show enhanced contextual fear memory without deficit in extinction of fear
Authors: Harter Aspen M. , Nemesh Mariya , Ji Michelle T. , Lee Luca , Yamazaki Anna , Kim Chris , Redei Eva E. 
Published: November 2024

AbstractThe prevalence of post‐traumatic stress disorder (PTSD) is higher in females than males, but pre‐clinical models are established almost exclusively in males. This study is aimed to investigate the stress‐enhanced fear learning model of PTSD in females. The model mirrors PTSD symptomology in males, whereby prior stress leads to extinction resistant exaggerated contextual fear memory. As stress reactivity is highly relevant to the study and risk for PTSD, females of the stress hyper‐reactive Wistar Kyoto More Immobile (WMI) and its nearly isogenic control the Wistar Kyoto Less Immobile (WLI) strains were employed. Prior studies have shown WMI females presenting unchanged or enhanced fear memory in the stress‐enhanced fear learning paradigm compared WLIs. The present study confirmed the enhanced fear memory following contextual fear conditioning in WMIs compared to WLI females, but this increased fear memory was neither exaggerated by prior stress nor showed extinction deficit. The novel stressor of a glucose challenge test resulted in subtle strain‐ and prior stress‐induced differences in plasma glucose responses. However, fasting plasma corticosterone levels were lower, and rose slower in response to glucose challenge in WMI females, suggesting a PTSD‐like dysfunctional stress response. Hippocampal expressions of genes relevant to both learning and memory and the stress response were decreased in stressed WMIs compared to WLI females, further suggesting a marked dysregulation in stress‐related functions like in PTSD. Thus, although WMI females do not show extinction‐resistant enhanced fear memory, they do present other characteristics that are relevant to PTSD in women.
Openness and transparency in animal research: Why and how
Authors: Genzel Lisa , Froudarakis Emmanouil , Rapti Georgia 
Published: November 2024

AbstractNonhuman animal models continue to be indispensable in neuroscience research for the foreseeable future. In recent years, animal‐right activists have been increasing the pressure on politicians and policymakers to phase out animal research. To address this pressure, we should adapt our communication habits, to be more open and transparent about (our) animal research and most importantly to expand the methods we use to communicate about our research and increase the extend of this outreach. In this editorial, we discuss the why and how of animal research communication.
Evolving Educational Testing to Meet Students’ Needs: Design‐in‐Real‐Time Assessment
Authors: Sireci Stephen G. , Suárez‐Álvarez Javier , Zenisky April L. , Oliveri Maria Elena 
Published: November 2024

AbstractThe goal in personalized assessment is to best fit the needs of each individual test taker, given the assessment purposes. Design‐In‐Real‐Time (DIRTy) assessment reflects the progressive evolution in testing from a single test, to an adaptive test, to an adaptive assessment system. In this article, we lay the foundation for DIRTy assessment and illustrate how it meets the complex needs of each individual learner. The assessment framework incorporates culturally responsive assessment principles, thus making it innovative with respect to both technology and equity. Key aspects are (a) assessment building blocks called “assessment task modules” (ATMs) linked to multiple content standards and skill domains, (b) gathering information on test takers’ characteristics and preferences and using this information to improve their testing experience, and (c) selecting, modifying, and compiling ATMs to create a personalized test that best meets the needs of the testing purpose and individual test taker.
Learning to care on one's own: Chinese children's caregivers' reflections on family care
Authors: Zhang Xue , Madureira Ferreira Juliene , Liu Jianjin , Zhang Qian , Midgette Allegra 
Published: November 2024

AbstractBackgroundThe past few decades have witnessed drastic changes in family structures and the provision of care within the family in China.ObjectiveThe current study investigated how familial caregivers of children (N = 30, Mage = 41.67, SD = 11.32) living in one of the largest urban centers in China learned and taught how to care within the family.MethodNine focus groups were conducted to collect data, and thematic analysis was adopted to analyze the data.ResultsTwo main themes were found: (a) learning to care on one's own and (b) the importance of encouraging one to want to care. The findings indicate that Chinese caregivers received limited direct instruction in learning how to care and were often faced with the challenge of having to self‐teach care. Meanwhile, when teaching care, caregivers focused on the motivation to care (e.g., filial piety) as an important element for learning to practice care.ConclusionThese findings highlight considerations of motivation of care often not mentioned in the Western literature while also indicating the need of society to support teaching and learning the practice of care within the family.ImplicationsThis study highlights the importance of cultural considerations when designing intervention programs to help individuals to develop the ability to care and provides insights exploring how school‐family partnership promotes children's ability to be caring.
The effects of death anxiety on diet‐fluid restriction non‐adherence in hemodialysis patients
Authors: Bahçecioğlu Turan Gülcan , Özer Zülfünaz , Başak Seda 
Published: November 2024

AbstractIntroductionThe study was performed to examine the effects of death anxiety on diet‐fluid restriction non‐adherence in hemodialysis patients.MethodsThis descriptive, cross‐sectional, and correlational study was performed with 118 hemodialysis patients who received treatment in the dialysis unit of a university hospital and a state hospital in Elazığ, eastern Turkey. The study data were obtained with the “Descriptive Data Form”, “Death Anxiety Scale”, and “Dietary and Fluid Restriction Non‐Adherence Scale”.FindingsThe average Death Anxiety Scale total score of the patients was 10.00 ± 4.75. The “Duration of non‐adherence with diet” mean score was 1.66 ± 1.70, “Degree of non‐adherence with diet” mean score was 1.24 ± 1.19, “Duration of non‐adherence with fluid restriction” mean score was 1.61 ± 1.71, and “Degree of non‐adherence with fluid restriction” mean score was 1.16 ± 1.13. The independent variable Death Anxiety Scale significantly and negatively affected the dependent variable “Duration of non‐adherence with diet”, “Degree of non‐adherence with diet”, “Duration of non‐adherence with fluid restriction”, and “Degree of non‐adherence with fluid restriction”.DiscussionHemodialysis patients face serious death anxiety, their degree of non‐adherence with diet‐fluid restriction was low as death anxiety scores increased, and the degree of non‐adherence with diet‐fluid restriction decreased.
Correlation between CTNNB1 mutation status and tumour phenotype in hepatitis B virus‐related hepatocellular carcinoma
Authors: Hwang Yoon Jung , Lee Yangkyu , Yu Su Jong , Hong Suk Kyun , Yi Nam‐Joon , Choi YoungRok , Lee Hyejung , Chung Wonju , Kim Haeryoung 
Published: November 2024

AimsThe frequency of CTNNB1 mutation, one of the most frequent genetic events in hepatocellular carcinoma (HCC), is lower in Asian countries and in hepatitis B virus (HBV)‐related HCCs. In this study, we evaluated the prevalence and types of CTNNB1‐mutation in HBV‐related HCC and correlated the molecular status with the histomorphological and immunohistochemical features.Methods and resultsA total of 108 consecutive cases of treatment‐naïve, surgically resected HBV‐related HCCs were selected. Targeted sequencing for CTNNB1 exons 3, 7 and 8 was performed, and the results were correlated with the expression pattern of glutamine synthetase (GS), nuclear β‐catenin expression status and the histomorphological characteristics of the tumour. CTNNB1 mutations were identified in 13% of HBV‐related HCCs; of these cases, mutations were found in D32‐S37 (7%), T41 (4%) and S45 (2%) of exon 3. None of the HCCs demonstrated alterations in exons 7 and 8. CTNNB1 mutation was strongly associated with diffuse strong GS expression (P < 0.001), nuclear β‐catenin expression (P < 0.001) and the classic CTNNB1 morphology (P = 0.038). Diffuse strong GS expression was observed in 78.6% of the CTNNB1‐mutated HCCs, and nuclear β‐catenin expression was identified in 64.3% of these cases. The classic CTNNB1 morphology was observed in 57% of all CTNNB1‐mutated HCCs. Furthermore, programmed death‐ligand 1 (PD‐L1) was less frequently expressed in HCCs with classic CTNNB1 morphology.ConclusionsCTNNB1 mutation was observed in 13% of HBV‐related HCCs in this Korean cohort, and was associated with diffuse strong GS expression, nuclear β‐catenin expression and classic CTNNB1 morphology.
Effectiveness of XBB.1.5 Vaccines Against Symptomatic SARS‐CoV‐2 Infection in Older Adults During the JN.1 Lineage‐Predominant Period, European VEBIS Primary Care Multicentre Study, 20 November 2023–1 March 2024
Authors: Merdrignac Lore , Laniece Delaunay Charlotte , Verdasca Nuno , Vega‐Piris Lorena , O'Donnell Joan , Sève Noémie , Trobajo‐Sanmartín Camino , Buda Silke , Hooiveld Mariëtte , Rodrigues Ana Paula , Túri Gergő , Latorre‐Margalef Neus , Mlinarić Ivan , Lazar Mihaela , Maurel Marine , Castrillejo Daniel , Bennett Charlene , Rameix‐Welti Marie‐Anne , Martínez‐Baz Iván , Dürrwald Ralf , Meijer Adam , Melo Aryse , Oroszi Beatrix , Hagey Tove Samuelsson , Kurečić Filipović Sanja , Dijkstra Frederika , Gomez Veronica , Bacci Sabrina , Kaczmarek Marlena , Kissling Esther ,   the VEBIS Primary Care Vaccine Effectiveness Group
Published: November 2024

ABSTRACTWe estimated XBB.1.5 vaccine effectiveness (VE) against symptomatic SARS‐CoV‐2 infection among adults aged ≥ 65 years during the 2023/2024 JN.1 lineage‐predominant period in a European multi‐country test‐negative case–control study at primary care level. We estimated VE adjusted by study site, age, sex, chronic conditions and onset date. We included 220 cases and 1733 controls. The VE was 48% (95% CI: 12–71), 23% (95% CI: −11–48) and 5% (95% CI: −92–56) among those with symptom onset 1–5, 6–11, and ≥ 12 weeks after vaccination, respectively. XBB.1.5 vaccine provided short and moderate protection against JN.1 symptomatic infection.
Multisource energy harvesting using (Ba,Ca)(Zr,Ti)O3 oscillating under temperature gradient
Authors: Yamamoto Ryota , Schwarz Michael , Martin Alexander , Mergheim Julia , Kakimoto Ken‐ichi 
Published: November 2024

AbstractThe concept of multisource energy harvesting has attracted attention in order to harvest multiple types of energy in a single material. In this work, Pb‐free (Ba,Ca)(Zr,Ti)O3 (BCZT) ceramics were used to investigate a combination of piezoelectric and pyroelectric energy harvesting behavior. The temperature dependence of the permittivity and the pyroelectric coefficient was measured, and the pyroelectric properties of BCZT were expected to improve near its orthorhombic–tetragonal phase transition temperature TO–T (∼40°C). The output voltage and current were measured using a BCZT plate, where the BCZT was oscillated under a temperature gradient to apply mechanical stress and temperature fluctuation simultaneously. The measured output voltage from the combined piezo‐ and pyroelectric contributions at a frequency of 2 Hz and with a load resistance of 10 MΩ was 1.4 times higher than the sole piezoelectric output voltage. The resulting combined output voltage was confirmed by finite element simulation.
Extending interfacial toughness and thermal cycling lifetime of thermal barrier coatings via interfacial 3D pattern
Authors: Luo Lirong , Wang Weize , Zhang Xiancheng , Lu Jie , Guo Fangwei , Zhao Xiaofeng 
Published: November 2024

AbstractWeak interface bonding is a critical factor that compromises the long‐term durability of the thermal barrier coatings. To mitigate this, we introduce an innovative interface‐strengthening approach by integrating four types of 3D patterns at the top coat/bond coat interface using the laser cladding technique. Tensile tests demonstrate a significant enhancement in interfacial bonding strength, increasing by over 139.2% from 21.6 ± 1.7 MPa for conventional thermal barrier coatings without patterns to 51.67 MPa for the patterned coatings. Moreover, the thermal cycling lifetime has been extended by 41%, from 1026 ± 25 cycles for the conventional coating to 1936 ± 164 cycles for the patterned coating. This enhancement in interfacial toughness and thermal cycling lifetime is primarily because of the blocking and deflection effects of 3D patterns on interfacial crack propagation. Besides, the geometric parameters of the patterns play a crucial role in determining the failure behavior of the thermal barrier coatings. This strategy presents a practical solution for the development of durable thermal barrier coatings and provides the valuable insights for the optimization of other heterogeneous interfaces.
Death and Dying in the Emergency Department: A New Model for End‐of‐Life Care
Authors: McCallum Kay , Jackson Debra , Walthall Helen , Aveyard Helen 
Published: November 2024

ABSTRACTBackgroundDeath and dying remain taboo subjects in society today and therefore people may come to the end of their life without having thought about what death and dying might be like and what it is to have a good death. The purpose of this qualitative study was to understand the experience of death and dying in a hospital emergency department. Culturally, some individuals are unprepared for death, and when death occurs in an emergency setting it can be particularly shocking.MethodsA phenomenological study was undertaken, based on the existential phenomenology of Merleau‐Ponty; and framed by the nurse theorist Hildegard Peplau. Bereaved relatives and registered nurses gave in‐depth interviews describing their experiences and the interviews were analysed using an adaptation of the work of Thomas and Pollio (2002) and Hycner (1985), consistent with Merleau‐Ponty's theories.ResultsThe study brings new understanding of what it is like to die in an emergency setting through new understanding of what the accompanying relatives/friends of the deceased person experience, aided by new understanding of the experiences of emergency nurses.ConclusionsA nursing model based both on the work of Peplau (Parse et al. 2000) and on the work of the nurse theorists Ruland and Moore (1998) and Zaccara et al. (2017) was devised for use in the emergency department when death occurs. It is hoped that this model will help nurses improve the care given to this group of patients and their loved ones.
Climate Justice Strategies Implemented by Public Health Nurses and Their Community Partners
Authors: LeClair Jessica , Dudek Alex , Zahner Susan 
Published: November 2024

ABSTRACTAimTo describe nurses' and community‐based organization representatives' collaborative strategies for advancing climate justice with communities.DesignThis study used a descriptive, qualitative research design.MethodsData were gathered from August 2022 to February 2023 with nurses (n = 8) and their community partners (n = 5) in the United States. Community partners were representatives of community‐based organizations. Photovoice provided greater context for the thematic analysis of collaborative strategies discussed in semi‐structured interviews.ResultsNurse participants worked in academic or non‐profit settings. Nurse‐community partnerships addressed corporate pollution and promoted Indigenous sovereignty and multispecies justice. Themes included investigating disease and health events, identifying at‐risk populations and connecting them with resources, providing health teaching and counseling, organizing communities and coalitions, and advocating for policy development and enforcement. Self‐care supported resilience and well‐being in the long struggle for climate justice.ConclusionFindings from this study indicated that nurses and their community partners strategize to transition communities away from systems of extraction towards local and regenerative systems that support resilience. Nurses and their community partners recognized the importance of applying an expansive understanding of climate justice, including intersections of pollution and multispecies justice, to promoting planetary health.Implications for the professionFindings from this study support nurse‐community collaboration in policy work to advance planetary health. This study also supports nurses' collective action with their community partners to address the effects of white supremacy and colonization. Future research is needed to evaluate the outcomes of nurse‐community partnerships for planetary health.ImpactNurses have called for action on climate justice; however, evidence of effective nursing strategies that advance climate justice is sparse. This study is the first to describe the collaborative strategies nurses implement with community partners to support the transition from injustice to justice in communities most burdened by climate change and industrial pollution.
Genetic Characterisation of Feeding Patterns in Lactating Holstein Cows and Their Association With Feed Efficiency Traits
Authors: Cavani Ligia , Parker Gaddis Kristen L. , Baldwin Ransom L. , Santos José E. P. , Koltes James E. , Tempelman Robert J. , VandeHaar Michael J. , White Heather M. , Peñagaricano Francisco , Weigel Kent A. 
Published: November 2024

ABSTRACTFeeding behaviour traits, such as number, duration or intake per feeder visit, have been associated with feed efficiency in dairy cattle. Those traits, however, do not fully capture cows' feeding patterns throughout the day. The goal of this study was to propose a new phenotype for characterising within‐day feeding patterns and estimate its heritability and genetic correlations with dry matter intake (DMI), secreted milk energy, metabolic body weight and residual feed intake. Feeding patterns were evaluated using 4.8 million bunk visits from 1684 midlactation Holstein cows collected from 2009 to 2023 with an Insentec system. Feed efficiency traits were available from 6099 lactating Holstein cows at six research stations across the United States. Daily bunk visits were ordered, with Time 0 designated as the time of first feed delivery. Intake proportions were calculated by visit for each cow by dividing feed intake per visit by the total intake of the cow for that day. Feeding patterns were characterised by the area under the curve of cumulative feed intake proportions for each cow throughout the day. The feeding pattern phenotype per cow was defined as the average of areas under the curve across days, whereas consistency of feeding pattern was calculated as the natural logarithm of variance of daily area under the curve values. Estimates of heritability and genetic correlations were performed using Bayesian inference with an animal model, considering lactation, days in milk and cohort (trial–treatment) as fixed effects and animal as a random effect. Heritability estimates for average area under the curve and variance of daily area under the curve were 0.35 ± 0.05 and 0.16 ± 0.05, respectively. The genetic correlation between average area under the curve and secreted milk energy was −0.30 ± 0.14. Genetic correlations between average area under the curve and DMI, metabolic body weight and residual feed intake were not statistically significant. Variance of daily area under the curve was genetically correlated with DMI (0.47 ± 0.15), secreted milk energy (0.40 ± 0.17) and metabolic body weight (0.28 ± 0.13). The genetic correlation between variance of daily area under the curve and residual feed intake was not significant. Overall, we provided a reliable method to truly characterise feeding patterns in midlactation dairy cows. Feeding pattern and its consistency were heritable, indicating that a significant proportion of phenotypic variation is explained by additive genetic effects. Genetic correlation estimates indicate that cows with more consistent daily feeding patterns have lower DMI, lower secreted milk energy and lower metabolic body weight.
Nitric oxide facilitates the S‐nitrosylation and deubiquitination of Notch1 protein to maintain cancer stem cells in human NSCLC
Authors: Zhang Tenglong , Lei Jiaxin , Zheng Ming , Wen Zhenke , Zhou Juying 
Published: November 2024

AbstractNon‐small cell lung cancer (NSCLC) is the leading cause of cancer‐related mortality, with tumour heterogeneity, fueled by cancer stem cells (CSCs), intricately linked to treatment resistance. Therefore, it is imperative to advance therapeutic strategies targeting CSCs in NSCLC. In this study, we utilized RNA sequencing to investigate metabolic pathway alterations in NSCLC CSCs and identified a crucial role of nitric oxide (NO) metabolism in governing CSC stemness, primarily through modulation of the Notch1 protein. Mechanistically, NO‐induced S‐nitrosylation of Notch1 facilitated its interaction with the deubiquitylase UCHL1, leading to increased Notch1 protein stability and enhanced CSC stemness. By inhibiting NO synthesis and downregulating UCHL1 expression, we validated the impact of NO on the Notch signalling pathway and CSC stemness. Importantly, targeting NO effectively reduced CSC populations within patient‐derived organoids (PDOs) during radiotherapy. This mechanism presents a promising therapeutic target to surmount radiotherapy resistance in NSCLC treatment.