bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiology of Shoulder and Elbow Injuries Among US High School Softball Players, 2005-2006 Through 2016-2017
Authors: Oliver Gretchen D. , Saper Michael G. , Drogosz Monika , Plummer Hillary A. , Arakkal Alan T. , Comstock R. Dawn , Anz Adam W. , Andrews James R. , Fleisig Glenn S. 
Published: September 2019

Background: Injury prevalence has been well described among baseball athletes; similarly, a better understanding of injuries in softball athletes is needed. Purpose: To examine shoulder and elbow injury epidemiology among high school softball athletes in the United States. Study Design: Descriptive epidemiological study. Methods: Injury data were obtained from the National High School Sports-Related Injury Surveillance System, which captures data from a large national sample of US high schools. Annually, a random sample of 100 high schools provided a representative sample with respect to the 4 US Census geographic regions and 2 school sizes (cutoff point, 1000 students). Athletic trainers from participating schools reported data for athlete-exposures (AEs; practice or competition) and shoulder and elbow injuries from 2005-2006 through 2016-2017. Results: A total of 239 shoulder injuries and 85 elbow injuries occurred within 2,095,329 AEs. The overall shoulder injury rate was 1.14 per 10,000 AEs, whereas the overall elbow injury rate was 0.41 per 10,000 AEs. Injuries to the shoulder were more likely to occur during competition as compared with practice (rate ratio, 1.28; 95% CI, 0.99-1.65). Half of the shoulder (50.4%) and elbow 48.9% injuries were due to an overuse/chronic mechanism. Of the athletes sustaining an injury, 86.8% with shoulder injuries and 93.0% with elbow injuries returned to play within 21 days. Only 16.7% of shoulder injuries and 17.5% of elbow injuries were sustained by pitchers. Conclusion: Shoulder and elbow injury rates, time to return, and percentage of injuries among pitchers were far lower in high school softball than previously reported values for high school baseball. There were relatively low incidences of shoulder and elbow injuries in high school softball as compared with baseball, with few injuries requiring lengthy time to return to play.
Fellow-Eye Conversion and Treatment in Exudative Age-Related Macular Degeneration
Authors: Dow Eliot R. , Adeghate Jennifer O. , Coombs Peter G. , Gupta Patel Mrinali , D’Amico Donald J. , Kiss Szilárd 
Published: September 2019

Purpose: This article assesses anatomical and visual outcomes after intravitreal antivascular endothelial growth factor (anti-VEGF) treatment in fellow eyes with wet age-related macular degeneration (AMD). Methods: A retrospective chart review was conducted of 349 patients diagnosed with wet AMD between 2005 and 2017 at a single academic institution. Initial diagnosis of unilateral wet AMD and a minimum follow-up time of 1 year after diagnosis were required for inclusion. Individuals were excluded if the initial diagnosis of wet AMD was made at an outside institution, if they had received prior treatment at another institution, or if they had bilateral wet AMD at the time of inclusion. Best-corrected visual acuity, optical coherence tomography (OCT) parameters, and frequency and type of intravitreal anti-VEGF injections were recorded. MedCalc Statistical Software version 17.6 (MedCalc Software) and GraphPad Software (GraphPad Software, Inc) were used for statistical analysis. Results: Of the 349 patients with wet AMD, 192 were included in the study (55%). Of these, 47 (24.5%) developed wet AMD in the fellow eye. The average time to second-eye conversion was 2.6 years, with fellow-eye conversion more likely to occur after 2 years of unilateral disease. On average, patients received 2.16 fewer injections per year in the fellow eye compared with the initially diagnosed eye ( P = .03), and fellow eyes tended to have better OCT results after 12 months of treatment. In addition, compared with ranibizumab and bevacizumab, aflibercept injections appeared to improve visual acuity both in initially diagnosed and fellow eyes. Conclusions: Exudative AMD in the fellow eye has a decreased treatment burden and better visual outcomes compared with the initial eye, which may be attributed to more frequent surveillance and early diagnosis.
Strong Valence Electrons Dependent and Logical Relations of Elemental Impurities in 2D Binary Semiconductor: a Case of GeP3 Monolayer from Ab Initio Studies
Authors: Zhang Suihao , Li Rui , Fu Xiaonan , Zhao Yu , Niu Chunyao , Li Chong , Zeng Zaiping , Wang Songyou , Xia Congxin , Jia Yu 
Published: September 2019

Abstract
Using first-principle calculations within density functional theory, we investigate the electronic property and stability of substitutionally doped 2D GeP3 monolayer with dopants from group III to VI. The conducting properties are found to be dramatically modified by both the doping sites and the number of valence electrons of dopants. Specifically, substitution on Ge site exhibits metal-semiconductor oscillations as a function of the number of valence electrons of dopants, while such oscillations are totally reversed when substitution on P site. Moreover, we also study the case of co-doping in GeP3, showing that co-doping can produce a logical “AND” phenomenon, that is, the conducting properties of co-doped GeP3 can be deduced via a simple logical relation according to the results of single doping. Finally, we investigate the formation energy of dopants and find that the electron-hole and hole-hole co-doped systems are much more energetically favorable due to the Coulomb attraction. Our findings not only present a comprehensive understanding of 2D doping phenomenon, but also propose an intriguing route to tune the electronic properties of 2D binary semiconductors.
Improving rice blast resistance of Feng39S through molecular marker-assisted backcrossing
Authors: Yang Dabing , Tang Jianhao , Yang Di , Chen Ying , Ali Jauhar , Mou Tongmin 
Published: September 2019

Abstract

Background
Rice blast caused by Magnaporthe oryzae is one of the most widespread biotic constraints that threaten rice production. Using major resistance genes for rice blast resistance improvement is considered to be an efficient and technically feasible approach to achieve optimal grain yield.

Results
We report here the introgression of the broad-spectrum blast resistance gene Pi2 into the genetic background of an elite PTGMS line, Feng39S, for enhancing it and its derived hybrid blast resistance through marker-assisted backcrossing (MABC) coupled with genomics-based background selection. Two PTGMS lines, designated as DB16206–34 and DB16206–38, stacking homozygous Pi2 were selected, and their genetic background had recurrent parent genome recovery of 99.67% detected by the SNP array RICE6K. DB16206–34 and DB16206–38 had high resistance frequency, with an average of 94.7%, when infected with 57 blast isolates over 2 years, and the resistance frequency of their derived hybrids ranged from 68.2% to 95.5% under inoculation of 22 blast isolates. The evaluation of results under natural blast epidemic field conditions showed that the selected PTGMS lines and their derived hybrids were resistant against leaf and neck blast. The characterizations of the critical temperature point of fertility-sterility alternation of the selected PTGMS lines, yield, main agronomic traits, and rice quality of the selected PTGMS lines and their hybrids were identical to those of the recurrent parent and its hybrids. DB16206–34/9311 or DB16206–38/9311 can be used as a blast-resistant version to replace the popular hybrid Fengliangyou 4. Likewise, DB16206–34/FXH No.1 or DB16206–38/FXH No.1 can also be used as a blast-resistant version to replace another popular hybrid Fengliangyou Xiang 1.

Conclusions
Our evaluation is the first successful case to apply MABC with genomics-based background selection to improve the blast resistance of PTGMS lines for two-line hybrid rice breeding.
Genetic dissection of yield-related traits and mid-parent heterosis for those traits in maize (Zea mays L.)
Authors: Yi Qiang , Liu Yinghong , Hou Xianbin , Zhang Xiangge , Li Hui , Zhang Junjie , Liu Hanmei , Hu Yufeng , Yu Guowu , Li Yangping , Wang Yongbin , Huang Yubi 
Published: September 2019

AbstractBackgroundUtilization of heterosis in maize could be critical in maize breeding for boosting grain yield. However, the genetic architecture of heterosis is not fully understood. To dissect the genetic basis of yield-related traits and heterosis in maize, 301 recombinant inbred lines derived from 08 to 641 × YE478 and 298 hybrids from the immortalized F2(IF2) population were used to map quantitative trait loci (QTLs) for nine yield-related traits and mid-parent heterosis.ResultsWe observed 156 QTLs, 28 pairs of loci with epistatic interaction, and 10 significant QTL × environment interactions in the inbred and hybrid mapping populations. The high heterosis in F1and IF2populations for kernel weight per ear (KWPE), ear weight per ear (EWPE), and kernel number per row (KNPR) matched the high percentages of QTLs (over 50%) for those traits exhibiting overdominance, whereas a notable predominance of loci with dominance effects (more than 70%) was observed for traits that show low heterosis such as cob weight per ear (CWPE), rate of kernel production (RKP), ear length (EL), ear diameter (ED), cob diameter, and row number (RN). The environmentally stable QTLqRKP3–2was identified across two mapping populations, whileqKWPE9, affecting the trait mean and the mid-parent heterosis (MPH) level, explained over 18% of phenotypic variations. Nine QTLs,qEWPE9–1,qEWPE10–1,qCWPE6,qEL8,qED2–2,qRN10–1,qKWPE9,qKWPE10–1, andqRKP4–3, accounted for over 10% of phenotypic variation. In addition, QTL mapping identified 95 QTLs that were gathered together and integrated into 33 QTL clusters on 10 chromosomes.ConclusionsThe results revealed that (1) the inheritance of yield-related traits and MPH in the heterotic pattern improved Reid (PA) × Tem-tropic I (PB) is trait-dependent; (2) a large proportion of loci showed dominance effects, whereas overdominance also contributed to MPH for KNPR, EWPE, and KWPE; (3) marker-assisted selection for markers at genomic regions 1.09–1.11, 2.04, 3.08–3.09, and 10.04–10.05 contributed to hybrid performance per se and heterosis and were repeatedly reported in previous studies using different heterotic patterns is recommended.
MBD1 promotes the malignant behavior of gallbladder cancer cells and induces chemotherapeutic resistance to gemcitabine
Authors: Wensheng Liu , Bo Zhang , Qiangsheng Hu , Wenyan Xu , Shunrong Ji , Jin Xu , Quanxing Ni , Xianjun Yu , Xiaowu Xu 
Published: September 2019

AbstractBackgroundMethyl-CpG binding domain protein 1 (MBD1), which couples DNA methylation to transcriptional repression, has been implicated in transcriptional regulation, heterochromatin formation, genomic stability, cell cycle progression and development. It has also been proven that MBD1 is involved in tumor development and progression. However, whether MBD1 is involved in tumorigenesis, especially in gallbladder cancer, is totally unknown.MethodsHuman GBC-SD and SGC996 cells were used to perform experiments. Invasion, wound healing and colony formation assays were performed to evaluate cell viability. A CCK-8 assay was performed to assess gallbladder cancer cell viability after gemcitabine treatment. Western blot analysis was used to evaluate changes in protein expression. Human gallbladder cancer tissues and adjacent nontumor tissues were subjected to immunohistochemical staining to detect protein expression.ResultsWe found that MBD1 expression was significantly upregulated in gallbladder cancer tissues compared with that in surrounding normal tissues according to immunohistochemical analysis of 84 surgically resected gallbladder cancer specimens. These data also indicated that higher MBD1 expression was correlated with lymph node metastasis and poor survival in gallbladder cancer patients. Overexpression and deletion in vitro validated MBD1 as a potent oncogene promoting malignant behaviors in gallbladder cancer cells, including invasion, proliferation and migration, as well as epithelial–mesenchymal transition. Studies have demonstrated that epithelial–mesenchymal transition is common in gallbladder cancer, and it is well known that drug resistance and epithelial–mesenchymal transition are very closely correlated. Herein, our data show that targeting MBD1 restored gallbladder cancer cell sensitivity to gemcitabine chemotherapy.ConclusionsTaken together, the results of our study revealed a novel function of MBD1 in gallbladder cancer tumor development and progression through participation in the gallbladder cancer epithelial–mesenchymal transition program, which is involved in resistance to gemcitabine chemotherapy. Thus, MBD1 may be a potential therapeutic target for gallbladder cancer.
Effect of hyperoside on cervical cancer cells and transcriptome analysis of differentially expressed genes
Authors: Guo Weikang , Yu Hui , Zhang Lu , Chen Xiuwei , Liu Yunduo , Wang Yaoxian , Zhang Yunyan 
Published: September 2019

AbstractBackgroundHyperoside (Hy) is a plant-derived quercetin 3-d-galactoside that exhibits inhibitory activities on various tumor types. The objective of the current study was to explore Hy effects on cervical cancer cell proliferation, and to perform a transcriptome analysis of differentially expressed genes.MethodsCervical cancer HeLa and C-33A cells were cultured and the effect of Hy treatment was determined using the Cell Counting Kit-8 (CCK-8) assay. After calculating the IC50 of Hy in HeLa and C-33A cells, the more sensitive to Hy treatment cell type was selected for RNA-Seq. Differentially expressed genes (DEGs) were identified by comparing gene expression between the Hy and control groups. Candidate genes were determined through DEG analysis, protein interaction network (PPI) construction, PPI module analysis, transcription factor (TF) prediction, TF-target network construction, and survival analysis. Finally, the key candidate genes were verified by RT-qPCR and western blot.ResultsHy inhibited HeLa and C33A cell proliferation in a dose- and time-dependent manner, as determined by the CCK-8 assay. Treatment of C-33A cells with 2 mM Hy was selected for the subsequent experiments. Compared with the control group, 754 upregulated and 509 downregulated genes were identified after RNA-Seq. After functional enrichment, 74 gene ontology biological processes and 43 Kyoto Encyclopedia of Genes and Genomes pathways were obtained. According to the protein interaction network (PPI), PPI module analysis, TF-target network construction, and survival analysis, the key genesMYC,CNKN1A,PAX2,TFRC,ACOX2,UNC5B,APBA1,PRKACA,PEAR1,COL12A1,CACNA1G,PEAR1, andCCNA2were detected. RT-qPCR was performed on the key genes, and Western blot was used to verifyC-MYCandTFRC.C-MYCandTFRCexpressions were lower and higher than the corresponding values in the control group, respectively, in accordance with the results from the RNA-Seq analysis.ConclusionHy inhibited HeLa and C-33A cell proliferation throughC-MYCgene expression reduction in C-33A cells andTFRCregulation. The results of the current study provide a theoretical basis for Hy treatment of cervical cancer.
An evaluation of risk-based monitoring in pragmatic trials in UK Clinical Trials Units
Authors: Beever Daniel , Swaby Lizzie 
Published: September 2019

Abstract

Background
Good Clinical Practice guidelines issued in 2016 encourage risk-based approaches to monitoring clinical trials. This study compared current risk assessment and monitoring approaches in UK Clinical Trials Units (CTUs) with the published guidance and makes recommendations for risk-based monitoring in pragmatic trials.

Methods
An online survey of UK Clinical Research Collaboration registered CTUs was administered via email invitation. Forty-nine units were invited, and 23 responded. Respondents were also invited to share copies of risk assessment templates.

Results
Most CTUs reported using remote combined with on-site monitoring. All reported undertaking a risk assessment for Clinical Trials of Investigational Medicinal Products (CTIMPs) and 21 units did so for non-CTIMPs. Most CTIMP risk assessments used MHRA (Medicines and Healthcare products Regulatory Agency) classifications, although some also employed staff judgement. Almost all units based their monitoring on perceived risk level; this number was higher for CTIMPs (n = 22) than for non-CTIMPs (n = 19). In most cases, monitoring plans were produced. More CTUs revisited risk assessments during trials in CTIMPs (n = 21) than in non-CTIMPs (n = 18). Small numbers of units reviewed the monitoring approach always (n = 4) or sometimes (n = 9) and few used the reflection to guide future monitoring.

Conclusions
A high proportion of UK CTUs are using risk-based monitoring in the UK, as recommended by guidelines, for both CTIMPs and non-CTIMPs. This has the potential to make trials more efficient and reduce costs. However, there appears to be a lack of reflection on the value of these revised approaches. There may be a benefit in CTUs collaborating nationally to improve processes for reflection and making changes during the life course of a trial.
Thickness of orthodontic clear aligners after thermoforming and after 10 days of intraoral exposure: a prospective clinical study
Authors: Bucci Rosaria , Rongo Roberto , Levatè Carmine , Michelotti Ambrosina , Barone Sandro , Razionale Armando Viviano , D’Antò Vincenzo 
Published: September 2019

Abstract

Background
Clear aligners (CA) are among the most chosen orthodontic therapies for patients who require an invisible treatment. Previous studies showed that the thermoforming process and the complexity of the intraoral environment might alter the properties of these devices. The aim of the current prospective clinical study was to assess the thickness changes of the CA after 10 days of intraoral use. The secondary aim was to assess the reproducibility of the thermoforming process, in terms of aligner thickness.

Materials and methods
CA from 18 consecutive patients (13 women, 5 men, mean age 28.8 ± 9.6 years) were investigated. Before intraoral exposure (T0), the thickness of the unused CA was measured at different occlusal points on a 3D model with a dedicated software (Geomagic Qualify 2013; 3D Systems, Rock Hill, SC, USA). Two CA configurations were studied: passive maxillary aligner (P—no tooth movement; no shape for attachments) and active maxillary aligner (A—tooth movement; shape for attachments and divot). The used aligners were returned after 10 days (T1) and the thickness measurements were repeated. A Student’s t test for paired data (T1 vs. T0) was applied to compare the thicknesses of used and unused devices (significance level after Bonferroni correction for multiple comparison was set at p < 0.0014). Furthermore, to study the reproducibility of the thermoforming process, P and A aligners were thermoformed twice, and the thicknesses of the two unused thermoformed devices were compared by means of Student’s t test for paired data (significance level after Bonferroni correction for multiple comparison was set at p < 0.0014) and Dahlberg’s error.

Results
The thermoforming process showed good reproducibility for both aligner configurations, with a maximum Dahlberg’s error of 0.13 mm. After intraoral use, the thickness of P showed some statistically significant, but not clinically relevant, thickness changes as compared to the unused aligners, while A did not show any significant changes.

Conclusion
Considering the thickness changes, the thermoforming process is reliable both with active and passive aligner configurations. Also, the CA examined show good thickness stability after physiological intraoral ageing in a population of healthy adults.
DESTINY: Database for the Effects of STellar encounters on dIsks and plaNetary sYstems
Authors: Bhandare Asmita , Pfalzner Susanne 
Published: September 2019

AbstractMost stars form as part of a stellar group. These young stars are mostly surrounded by a disk from which potentially a planetary system might form. Both, the disk and later on the planetary system, may be affected by the cluster environment due to close fly-bys. The here presented database can be used to determine the gravitational effect of such fly-bys on non-viscous disks and planetary systems. The database contains data for fly-by scenarios spanning mass ratios between the perturber and host star from 0.3 to 50.0, periastron distances from 30 au to 1000 au, orbital inclination from 0∘ to 180∘ and angle of periastron of 0∘, 45∘ and 90∘. Thus covering a wide parameter space relevant for fly-bys in stellar clusters. The data can either be downloaded to perform one’s own diagnostics like for e.g. determining disk size, disk mass, etc. after specific encounters, obtain parameter dependencies or the different particle properties can be visualized interactively. Currently the database is restricted to fly-bys on parabolic orbits, but it will be extended to hyperbolic orbits in the future. All of the data from this extensive parameter study is now publicly available as DESTINY.
Data-driven elastic modeling of organic-rich marl during maturation
Authors: Suwannasri Krongrath , Vanorio Tiziana , Clark Anthony C. 
Published: September 2019

The elastic modeling of source-rock reservoirs during maturation must incorporate microstructural and geochemical alterations. The common challenge is calibrating the volumetric proportion between each form of organic porosity along with the changes in the bulk and shear moduli of kerogen as a function of maturity. Two forms of organic porosity have generally been observed: (1) spongy and bubble pores inside kerogen and (2) low-aspect-ratio pores or gaps at the interface between shrinking kerogen bodies and the matrix. We have constructed a rock-physics model of organic-rich marl during maturation and calibrated it using rock-physics data sets from controlled pyrolysis experiments of organic-rich marl under stress. We chose these pyrolysis data because the samples provide subsequent changes in porosity and P- and S-wave velocities as a function of maturity, while evidencing minimal grain sliding and mechanical compaction due to their stiff matrix. Our calibration results indicate that spongy and bubble pores should be used as the predominant form in the model regardless of maturity. Our results also indicate a competing effect between increasing kerogen porosity and the increasing moduli of solid kerogen. Kerogen porosity mainly develops throughout the oil windows. Whereas the moduli of solid kerogen increase by a factor of two in the early-peak oil window and remain relatively constant afterward. Consequently, the effective moduli of kerogen experience minimal changes in the early-peak oil window and rapidly decrease to half of the immature values in the late oil window. These calibration results are consistent with several petrophysical and nanoindentation studies on kerogen. Finally, we used the calibrated model to build a rock-physics template of organic-rich marl during maturation. The template was tested with pyrolyzed and naturally matured samples, which showed that our model can be used to characterize reservoir properties across different maturity windows.