bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ancient mitochondrial DNA connects house mice in the British Isles to trade across Europe over three millennia
Authors: García-Rodríguez Oxala , Hardouin Emilie A , Hambleton Ellen , Monteith Jonathan , Randall Clare , Richards Martin B , Edwards Ceiridwen J , Stewart John R 
Published: September 2019

Abstract
The earliest records in Britain for the western house mouse ( Mus musculus domesticus ) date from the Late Bronze Age. As a commensal species, the arrival to Britain is suggested to be related with human transport and trade. In order to study the arrival of the species in Britain, we collected a total of 16 ancient mouse mandibulae from some of the rare early sites with the species in the four British archaeological sites, ranging from the Late Bronze Age to the Roman period. From these, we obtained the first ancient mitochondrial DNA (mtDNA) house mouse sequences from the British Isles, including eight house mice from three different archaeological sites ranging from the Late Bronze Age to the Middle Iron Age. We also obtained five ancient mtDNA wood mouse ( Apodemus spp.) sequences from across all four sites. Modern British house mouse mtDNAs are primarily characterised by haplogroups E, F and, much less commonly, haplogroup D. The presence of haplogroups D and E in our samples provides evidence of an early house mouse colonisation that may be related with Late Bronze Age/Iron Age trade and/or expansions of humans. Our results confirm the hypothesis, based on zooarchaeological evidence and modern mtDNA predictions, that house mice with haplogroups D and E were established in Britain by the Iron Age and, in the case of haplogroup E, possibly even by the Late Bronze Age.
Antimicrobial Resistance of Escherichia coli and Salmonella isolated from Raw Retail Broiler Chickens in Zambia
Authors: Muonga Elizabeth Muligisa , Mainda Geoffrey , Mukuma Mercy , Kwenda Geoffrey , Hang'ombe Bernard , Phiri Nelson , Mwansa Mwaba , Munyeme Musso , Muma John Bwalya 
Published: September 2019

Abstract
Background Antimicrobial resistance (AMR) of foodborne pathogens is of public health concern, especially in developing countries like Zambia. This study was undertaken to determine the resistance profiles of Escherichia coli ( E. coli ) and Salmonella isolated from dressed broiler chickens purchased from open markets and supermarkets in Zambia.Results A total of 189 E. coli and five Salmonella isolates were isolated. Identification and confirmation of the isolates was done using Analytical Profile Index (API 20E) (Biomerieux ® ) and 16S rRNA sequencing. Antimicrobial susceptibility tests (AST) were performed using the Kirby Bauer disk diffusion technique using a panel of 10 different antibiotics and multiplex PCR was used to determine the presence of three target genes encoding for resistance: tetA, Sul1 and CTXM. AST results were entered and analyzed in WHONET 2018 software. A total of 189 E. coli and five Salmonella isolates were identified. Among the E. coli isolates, Tetracycline recorded the highest resistance of 79.4%, followed by Ampicillin 51.9%, Trimethoprim/Sulfamethoxazole 49.7%, Nalidixic Acid 24.3%, Chloramphenicol 16.4%, Cefotaxime 16.4%, Ciprofloxacin 10.1%, Colistin 7.4%, Amoxicillin/Clavulanic acid 6.9%, and Imipenem 1.1%. Two of the five Salmonella isolates were resistant to at least one antibiotic. Forty- seven (45.2%) of the isolates possessed at least one of the targeted resistance genes.Conclusion This study has demonstrated the presence of AMR E. coli and Salmonella on raw broiler chickens from both open markets and supermarkets. Such resistance is of public health concern and measures need to be put in place to regulate the use of these antimicrobials in poultry production.
Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells
Authors: Mihevc Sonja Prpar , Grgich Vesna Kokondoska , Kopitar Andreja Nataša , Mohorič Luka , Majdic Gregor 
Published: September 2019

Abstract
Background: Ability of adipose tissue-derived multipotent mesenchymal stromal cells/mesenchymal stem cells (ASCs) to differentiate in neural lineages promises progress in the field of regenerative medicine especially for replacing damaged neuronal tissue in different neurological disorders. Reprogramming of ASCs can be induced by supplying growth medium with chemical neurogenic inductors and/or specific growth factors. We investigated the neural differentiation potential of canine ASCs using several growth media (KEM, NIMa, NIMb, NIMc) containing various combinations of neurogenic inductors: B27 supplement, valproic acid, forskolin, N2-supplement and retinoic acid. First the cells were preconditioned in proliferation medium, followed by induction of neuronal differentiation. Six canine ASCs cell lines were assessed, half from female and half from male donors. The cell morphology, growth dynamics, viability were observed along with expression of neuron and astrocyte specific markers, which were assessed by immunocytochemistry and flow cytometry.
Results: After 3, 6 and 9 days, elongated neural-like cells with bipolar elongations were observed and some oval cells with light nuclei appeared. After three and nine days of neural induction, differentiation into neurons and glial cells was observed. Expression of neuronal markers tubulin beta III (TUBB3), neurofilament H (NF-H) and glial fibrillary acidic protein (GFAP) was observed by immunocytochemistry. High GFAP expression (between 70 and 90% of all cells) was detected after three days of growth in neural induction medium a (NIMa) by flow cytometry, and expression of adult neuronal markers NF-H and microtubule associated protein-2 (MAP2) was detected in around 25% of cells. After nine days of ASCs differentiation a drop in expression rates of all markers was detected. There were no differences between neural differentiation of ASCs isolated from female or male dogs.
Conclusions: The differentiation repertoire of canine ASCs extends beyond mesodermal lineages. Using a defined neural induction medium the canine ASCs were able to transform to neural lineages, bearing markers of neuronal and glial cells and also displayed the typical neuronal morphology. Differentiated ASCs can be a source of neural cellular lineages for regenerative therapy of nerve damage and also could be applicable for modeling of neurodegenerative diseases.
Near-infrared auto-fluorescence spectroscopy combining with Fisher’s linear discriminant analysis improves intraoperative real-time identification of normal parathyroid in thyroidectomy
Authors: Liu Junsong , Wang Xiaoxia , Wang Rui , Xu Chongwen , Zhao Ruimin , Li Honghui , Zhang Shaoqiang , Yao Xiaobao 
Published: September 2019

Abstract
Background To evaluate the efficacy of a sensitive, real-time tool for identification and protection for parathyroid glands during thyroidectomy.Methods NIR fluorescence was measured intraoperatively from 20 patients undergoing thyroidectomy. Spectra were measured from suspicious parathyroid glands and surrounding neck tissues during the operation with a NIR fluorescence system. Fast frozen sections were performed on the suspicious parathyroid glands. Accuracy was evaluated by comparison with histology or NIR recognition. Data were attracted for Fisher’s linear discriminant analysis.Results The auto-fluorescence intensity of parathyroid was significantly higher than that of thyroid, fat and lymph node. The peak intensity of auto-fluorescence from parathyroid was 5.55 times of that from thyroid at the corresponding wave number. Of the 20 patients, the parathyroid was accurately detected and identified in 19 patients by NIR system, compared with their pathologic findings. One suspicious parathyroid did not exhibit typical spectra, and was proved to be fat tissue by pathology. The parathyroid gland have specific auto-fluorescence spectrum and can be separated from the other three samples through the Fisher’s linear discriminant analysis.Conclusions NIR auto-fluorescence spectroscopy can accurately identify normal parathyroid gland during thyroidectomy. The Fisher’s linear discriminant analysis demonstrated the specificity of the NIR auto-fluorescence of parathyroid tissue and its efficacy in parathyroid discrimination.
Application of Iconport single incision sealing device in laparoscopic surgery.(Experience in 125 cases of appendectomy)
Authors: Wang Xiaojun , Jin Haibo , Shuai YongFeng , Zhang YiZhong 
Published: September 2019

Abstract
Background To discuss the reliability and clinical value of Iconport, a Self-made single port device, which was applied for transumbilical single port laparoscopic appendectomy. Methods The incision was around 2.5 cm long with curved form around umbilicus. And the skin and each layer of the abdominal wall were cut in sequence. Then the silica gel sleeve of the self-made single-incision sealing device was folded, clamped by the forceps, and carefully inserted into the incision for sealing. The operation of laparoscopic appendectomy was accomplished by-use routine linetype laparoscopic instruments via Iconport. The specimen was discharged from peritoneal cavity through the single port device. Two layers of incision, linea alba abdominus and skin, were separately sutured by absorbable thread and each layer was sutured successively. Results Among the 125 patients, 119 were successfully performed single-port laparoscopic appendectomy, with the operation time ranging from 30 to 110 min (69.34±21.4 min on average). In 6 cases, auxiliary operating hole was added or laparotomy was performed. Total hospital stay was 5.0±1.8 (2-14) days. 5 cases of incision infection. All patients were followed up for 1 to 6 months after surgery, with an average of 3 months. There were no complications such as bleeding, intestinal leakage, intestinal obstruction, and residual peritoneal effusion. Conclusion In single-incision laparoscopic surgery, the u-shaped incision around the umbilical margin of 2.5cm for adult can not only meet the requirements of flexibility of instruments in the operation, but also meet the aesthetic requirements because the incision scar is hidden in the umbilical foramen or looks like the umbilical foramen after the operation. Iconport single-hole device, which has potential promotion value, can be used in relatively simple laparoscopic surgery alone or combined with a single puncture device for relatively complex laparoscopic surgery.
Compare of Sequential, Delayed and Simultaneous Resection Strategies for Synchronous Colorectal Liver Metastases
Authors: Wang Li-Jun , Wang Hong-Wei , Jin Ke-Min , Li Juan , Xing Bao-Cai 
Published: September 2019

Abstract
Background: The present study aimed to compare perioperative safety and long-term survival of patients with synchronous colorectal liver metastases receiving sequential resection (SeR), delayed resection (DeR) and simultaneous resection (SiR).
Methods: From January 2007 to December 2016, data of patients receiving surgery at Peking University Cancer Hospital for synchronous colorectal liver metastases were retrospectively collected. The above three different surgical strategies were compared.
Results: A total of 233 cases were included, with 49 in the SeR group, 98 in the DeR group and 86 in the SiR group. The incidence of severe complications was 26.7% in the SiR group, higher than that in the DeR group (11.2%, p=0.007) and the SeR group (16.3%, p=0.166). Overall survival at 1-year and 3-year in the SeR group (93.9% and 50.1%) was lower than the DeR group (94.9% and 64.8%, p=0.019), but not statistically different compared with the SiR group (93.0% and 55.2%, p=0.378). Recurrence-free survival at 1-year and 3-year in the SeR group (22.4% and 18.4%) was lower than the DeR group (43.9% and 24.2%, p=0.033), but not statistically different compared with the SiR group (31.4% and 19.6%, p=0.275). Cox multivariate analysis indicated that T4, lymph node positive primary tumor, liver metastases>30mm and selective sequential resection (compared with delayed resection) were correlated with poor prognosis.
Conclusion: Simultaneous resection has a relatively higher incidence of severe complication, and when staged resection strategy was made, the prognosis of delayed resection was better than that of sequential resection.
Survival outcomes of lobectomy in comparison with segmentectomy in clinical stage I non-small-cell lung cancer: a meta-analysis
Authors: Feng Jian , Han Yan-Yue , Wang Yue , Wu Xiu-Yu , Lv Feng , Liu Yang , Chen Bing-Hui 
Published: September 2019

Abstract
Background: The gold standard surgical therapy for patients with Clinical stage I non-small-cell lung cancer (NSCLC) is lobectomy with mediastinal lymph node dissection. While, segmentectomy has emerged as an alternative choice with the advantage of fewer postoperative complications. While the acceptance of this procedure still remains controversial, and conflicting results exist in the retrospective trials. Objectives: The aim of this meta-analysis was to analysis the survival outcomes of Lobectomy in comparison with segmentectomy in treatment of Clinical stage I non-small-cell lung cancer. Methods: Computerized literature search was done on the published trials in Pubmed, Embase, Cochrane library databases to June, 2019 to identify clinical trials. Lung cancer-specific survival (LCSS) and overall survival (OS) were measured as outcomes. Statistical analysis was performed in the Meta-analysis Revman 5.3 software. Results: A systematic literature search was conducted including 7 studies. In this meta-analysis, results indicate that lobectomy confers an equivalent survival outcome compared with segmentectomy. Conclusion: No significant differences were found in survival outcomes between lobectomy and segmentectomy. Further prospective large-scale, prospective, randomized trials are needed to explore the reasonable surgical treatment for early resectable lung cancer.
Distant mood monitoring for psychiatric disorders: A systematic review
Authors: Watt Alberta Susanna Johanna van der , Odendaal Willem , Louw Kerry , Seedat Soraya 
Published: September 2019

Abstract
Background: Whilst electronic self-monitoring and intervention programmes for mood disturbances in psychiatric disorders may promote self-management and patient empowerment, some level of interaction with professionals (such as clinicians, counsellors, and researchers) coupled with support is still positively valued by patients. This can allow for a more personalised approach, improve the efficiency of treatment, and adverse events can be managed in a time-appropriate manner, thereby mitigating some of the risks associated with mood fluctuations. Methods: This systematic review synthesises quantitative and qualitative evidence on the effectiveness and feasibility of daily/weekly/monthly remote mood monitoring by distant supporters (clinicians, lay counsellors, and researchers) (or with regular feedback by distant supporters in cases where mood monitoring was self-assessed), in participants with any psychiatric disorder. Effectiveness was defined by the change in depression and/or mania scores. Feasibility was determined according to completion/attrition rates and participant feedback. Studies were assessed for quality using the Mixed Methods Appraisal Tool (MMAT) version 2018. Results: Eight studies met our inclusion criteria. Distant mood monitoring was effective in improving depression scores but not mania scores. Feasibility, as measured through compliance and completion rates and participant feedback, varied. Conclusion: Distant mood monitoring with feedback is an appealing intervention, particularly in low resourced settings; however, further studies are needed to better understand the utility, feasibility, and effectiveness of these interventions in routine clinical care.
From Folk Therapy to Evidence-based Psychiatry Practice: The Benefit of Evidence-based Psychiatry in Treatment-naive Psychotic Patients
Authors: Wang Hung-Yu , Huang Joh-Jong , Su Shu-Fang , Hsu Sheng-Hao , Chou Li-Shiu , Chou Frank 
Published: September 2019

Abstract
Background: Though Taiwan’s Mental Health Act clearly states that the human rights and legal rights of psychotic patients should be respected and guaranteed, a temple mental asylum violated those regulations in the 21st century. Hundreds of patients were constrained in the asylum and evacuated following a forcible, public right intervention. Method: The study conducted drug-naïve psychotic patients constrained in an asylum for decades. Before and after formal treatment, a total of 169 patients diagnosed with schizophrenia and other psychotic disorders were assessed with rating scales including the Mini Positive and Negative Syndrome Scale, Comprehensive Occupational Therapy Evaluation scale. In addition, family function, self-care and nutritional status were evaluated. Results: The initial data show improvement in psychotic symptoms and occupational function. Furthermore, the ratio of patients classified as being at risk for malnutrition was decreased by 21.7% after treatment. Conclusion: The psychotic symptoms and occupational function of these patients improved after undergoing formal treatment compared with folk therapy. The care model for psychotic patients in the temple asylum should be discussed while considering medical ethics principles. Trial registration: retrospectively registered.
Efficacy and clinical predictors of response to rTMS treatment in obsessive-compulsive disorder (OCD): A retrospective study
Authors: Rostami Reza , Kazemi Reza , Jabbari Arezoo , Madani Azam , Rostami Hosseinreza , Taherpour Mohammad Amin , Jaafari Nematollah , Kuo Min-Fang , Vicario Carmelo M. , Nitsche Michael A. , Salehinejad Mohammad Ali 
Published: September 2019

Abstract
Background: Application of the repetitive transcranial magnetic stimulation (rTMS) for treating obsessive-compulsive disorder (OCD) has been promising but effects differ between patients. Knowledge about clinical predictors of rTMS response would help to increase clinical efficacy but is not available so far. Methods: In a retrospective study, we investigated the efficacy of rTMS over the dorsolateral prefrontal cortex (DLPFC) or supplementary motor area (SMA) in 65 OCD outpatients recruited for the rTMS treatment from July 2015 to May 2017. Patients were divided into two groups and received either SMA rTMS or bilateral DLPFC rTMS. OCD symptoms and depression/anxiety states were measured before and after the 20th session of rTMS. Additionally, we performed a binary logistic regression analysis on the demographic variables and the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS) items to investigate demographic and clinical predictors for rTMS response in responders and non-responders. Results: Patients’ scores in Y-BOCS and Beck anxiety/depression inventories were significantly decreased following rTMS treatment. Specifically, 46.2% of all patients responded to rTMS, based on at least 30% reduction of the Y-BOCS scores. Stimulation target (DLPFC vs. SMA) did not significantly differ in rTMS efficacy. No significant demographic predictors were found. Interference due to obsessions and resistance against compulsions were the only two clinical predictors of rTMS treatment response that could significantly predict response failure to rTMS. Conclusions: rTMS treatment should be adapted to the clinical profile of OCD patients including the symptoms. Patients with less intrusive and interfering thoughts might benefit more from rTMS treatment. Identifying clinical and non-clinical predictors of response are needed to personalize and adapt rTMS protocols in pharmaco-resistant OCD patients.
Overexpression of LncRNA-PVT1 promotes proliferation migration, invasion and fibrosis in diabetic nephropathy via suppressing miR-93-5p
Authors: Li Jianzhou , Zhao Qing , Jin Xiaohong , Li Yanhua , Song Jian 
Published: September 2019

Abstract
Background : This research aimed to explore the molecular mechanism of LncRNA- plasmacytoma variant translocation 1 (PVT1) in diabetic nephropathy (DN). Methods: Mouse mesangial cells (MMCs) were obtained from diabetic model mice. The real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), Western blot, luciferase reporter gene assay, EdU assay, flow cytometry analysis, AnnexinV-PI double staining, scratch assay, Transwell assay, enzyme-linked immunosorbent assay (ELISA) and luciferase reporter gene assay were performed to reveal the effect of PVT1 on the proliferation, migration, invasion and fibrosis of MMCs cultured in high glucose. Results: PVT1 was overexpressed in both mice kidney tissue and high glucose cultured MMCs. Meanwhile, PVT1 could not only promoted proliferation, migration, invasion and fibrosis of high glucose cultured MMCs, but also regulated the cell cycle from G0/G1 to S phase. Moreover, PVT1 negatively regulated the expression of miR-93-5p in MMCs. Furthermore, the PVT1-miR-93-5p regulatory relation activated the PI3K/Akt/mTOR pathway, which in turn regulated cell proliferation and insulin signaling in high glucose cultured MMCs. Conclusions: PVT1 might promoted proliferation, migration, invasion and fibrosis in high glucose cultured MMCs, which further affected the development of DN. Furthermore, PVT1-miR-93-5p regulatory relation might take part in DN progression via activating the PI3K/Akt/mTOR pathway.
The Value of ESWAN in Diagnostic of Adenomyosis and Related Study about Dysmenorrheal
Authors: Cheng Qi-Chao , Wang Zong-Ying , Li Fei , Li Feng-Zhi , Xin Jian-Ying , Gao Shan-Shan , Liu Jin-Gang , Chen Chang-Sheng , Wang Xizhen 
Published: September 2019

Abstract
Background This retrospective study aimed (1) to evaluate the value of enhanced T2 star-weighted angiography (ESWAN) in diagnosis Adenomyosis(AM). And (2) to estimate the differences of focal AM patients lesion and the peripheral tissues region. And (3) analysis the correlation between the multiple indices of ESWAN and dysmenorrheal, focal AM lesion ratio.Methods Traditional MRI and ESWAN were performed on seventy-two clinically–diagnosed AM patients (fifty for focal and twenty-two for diffuse AM), and fifty-six normal adult woman. The ESWAN indices (magnitude value, phase value, R2* value and T2* value) measured on different regions of interest (ROIs) were analyzed. Measured the focal AM lesion size and Uterine volume by tradition MRI, and using visual analogue scale(VAS) measured dysmenorrheal level.Results The magnitude value, phase value, R2* value and T2* value of focal AM, diffuse AM, peripheral tissue in focal AM and normal myometrium were respectively 1176.20±367.70/1286.51±349.49/1139.61±426.69/1685.94±407.14, 0.0114±0.0438/0.0138±0.0182/0.0296±0.0858/0.0433±0.0869, 26.39±3.36/27.40±2.36/27.24±4.60/22.54±5.03, 39.83±5.47/37.49±3.48/38.67±7.09/43.39±8.94. The ESWAN indices by one-way ANOVA showed statistical difference with the normal myometrium and focal and diffuse AM(0.000, 0.027, 0.000, 0.002). Compared with the peripheral tissue, the focal AM indices were no statistical difference. The correlation between the dysmenorrheal level and phase value were statistical difference(0.018).Conclusions ESWAN sequence has fractional value in diagnosis AM by different indices. And the dysmenorrheal level could react the AM feature in certain degree.