bluuebunny/crossref_metadata_2025_split huggingface dataset:
The EBI2-oxysterol axis promotes the development of intestinal lymphoid structures and colitis
Authors: Wyss Annika , Raselli Tina , Schmelczer Gérard , Klinke Glynis , Perkins Nathan , Hersberger Martin , Spalinger Marianne R. , Atrott Kirstin , Lang Silvia , Frey-Wagner Isabelle , Scharl Michael , Sailer Andreas W. , Pabst Oliver , Rogler Gerhard , Misselwitz Benjamin 
Published: September 2018

AbstractThe gene encoding for Epstein-Barr virus-induced G-protein coupled receptor 2 (EBI2) is a risk gene for inflammatory bowel disease (IBD). Together with its oxysterol ligand 7α,25-dihydroxycholesterol, EBI2 mediates migration and differentiation of immune cells. However, the role of EBI2 in the colonic immune system remains insufficiently studied.We found increased mRNA expression of EBI2 and oxysterol synthesizing enzymes (CH25H, CYP7B1) in the inflamed colon of patients with ulcerative colitis and mice with acute or chronic dextran sulfate sodium (DSS) colitis. Accordingly, we detected elevated extraintestinal levels of 25-hydroxylated oxysterols, including 7α,25-dihydroxycholesterol in mice with acute colonic inflammation. Knockout of EBI2 or CH25H did not affect severity of DSS colitis; however, inflammation was decreased in male EBI2-/- mice in the IL-10 colitis model.The colonic immune system comprises mucosal lymphoid structures, which accumulate upon chronic inflammation in IL-10-deficient mice and in chronic DSS colitis. However, EBI2-/- mice formed significantly less colonic lymphoid structures at baseline and showed defects in inflammation-induced accumulation of lymphoid structures.In summary, we report induction of the EBI2-7α,25-dihydroxycholesterol axis in colitis and a role of EBI2 for the accumulation of lymphoid tissue during homeostasis and inflammation. These data implicate the EBI2-7α,25-dihydroxycholesterol axis in IBD pathogenesis.
Endoglycan plays a role in axon guidance and neuronal migration by negatively regulating cell-cell adhesion
Authors: Baeriswyl Thomas , Dumoulin Alexandre , Schaettin Martina , Tsapara Georgia , Niederkofler Vera , Helbling Denise , Avilés Evelyn , Frei Jeannine A. , Wilson Nicole H. , Gesemann Matthias , Stoeckli Esther T. 
Published: September 2018

SUMMARYCell migration and axon guidance are important steps in the formation of neural circuits. Both steps depend on the interactions between cell surface receptors and molecules on cells along the pathway. In addition to cell-cell adhesion, these molecular interactions provide guidance information. The fine-tuning of cell-cell adhesion is an important aspect of cell migration, axon guidance, and synapse formation.Here, we show that Endoglycan, a sialomucin, plays a role in axon guidance and cell migration in the central nervous system. In the absence of Endoglycan, commissural axons failed to properly navigate the midline of the spinal cord. In the developing cerebellum, a lack of Endoglycan prevented migration of Purkinje cells and resulted in a stunted growth of the cerebellar lobes. Taken together, these results support the hypothesis that Endoglycan acts as a ‘lubricant’, a negative regulator of cell-cell adhesion, in both commissural axon guidance and Purkinje cell migration.
phylogenize:correcting for phylogeny reveals genes associated with microbial distributions
Authors: Bradley Patrick H. , Pollard Katherine S. 
Published: September 2018

AbstractSummaryPhylogenetic comparative methods are powerful but presently under-utilized ways to identify microbial genes underlying differences in community composition. These methods help to identify functionally important genes because they test for associations beyond those expected when related microbes occupy similar environments. We presentphylogenize, a pipeline with web, QIIME2, and R interfaces that allows researchers to perform phylogenetic regression on 16S amplicon and shotgun sequencing data and to visualize results.phylogenizeapplies broadly to both host-associated and environmental microbiomes. Using Human Microbiome Project and Earth Microbiome Project data, we show thatphylogenizedraws similar conclusions from 16S versus shotgun sequencing and reveals both known and candidate pathways associated with host colonization.Availabilityphylogenizeis available athttps://phylogenize.organdhttps://bitbucket.org/pbradz/phylogenize.Contactkpollard@gladstone.ucsf.edu
MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo
Authors: Bayraktar Erol Can , Baudrier Lou , Özerdem Ceren , Lewis Caroline A. , Chan Sze Ham , Kunchok Tenzin , Abu-Remaileh Monther , Cangelosi Andrew L. , Sabatini David M. , Birsoy Kivanc , Chen Walter W. 
Published: September 2018

ABSTRACTMitochondria are metabolic organelles that are essential for mammalian life, but the dynamics of mitochondrial metabolism within mammalian tissues in vivo remains incompletely understood. While whole-tissue metabolite profiling has been useful for studying metabolism in vivo, such an approach lacks resolution at the cellular and subcellular level. In vivo methods for interrogating organellar metabolites in specific cell-types within mammalian tissues have been limited. To address this, we built on prior work in which we exploited a mitochondrially-localized 3XHA epitope-tag (“MITO-Tag”) for the fast isolation of mitochondria from cultured cells to now generate “MITO-Tag Mice.” Affording spatiotemporal control over MITO-Tag expression, these transgenic animals enable the rapid, cell-type-specific immunoisolation of mitochondria from tissues, which we verified using a combination of proteomic and metabolomic approaches. Using MITO-Tag Mice and targeted and untargeted metabolite profiling, we identified changes during fasted and refed conditions in a diverse array of mitochondrial metabolites in hepatocytes and found metabolites that behaved differently at the mitochondrial versus whole-tissue level. MITO-Tag Mice should have utility for studying mitochondrial physiology and our strategy should be generally applicable for studying other mammalian organelles in specific cell-types in vivo.
Potassium nutrition recover impacts on stomatal, mesophyll and biochemical limitations to photosynthesis in Carya cathayensis and Hickory illinoensis
Authors: Shen Chao , Huang Ruimin , Tang Yiquan , Wang Zhengjia 
Published: September 2018

AbstractPotassium (K) influences the photosynthesis process in a number of ways; However, the mechanism of photosynthetic response to the long-term supply of potassium is not yet clear. Concurrent measurements of gas exchange and chlorophyll fluorescence were made to investigate the effect of potassium nutrition on photosynthetic efficiency and stomatal conductance (gs), mesophyll conductance (gm) in Pecan (Carya illinoensis K.Kock) and Hickory (Carya cathayensis Sarg.) seedlings in a greenhouse. The results show that the photosynthetic capacity of Pecan and Hickory plants was not limited when the leaves had potassium concentrations >1.4% and 1.42% of dry weight. Most of limitation under potassium deficiency were dominated by MCL for Pecan and Hickory. Both cultivars showed remarkable improvement in SL, MCL, J and Vc,max with additional K supplies. However, effect from potassium deficiency on photosynthesis in plant leaves was irreversible. All of SL, MCL, and BL nearly half down with recovery K supply in both species. These results emphasize the important role of potassium on regulation of photosynthesis by three limitations.
Long-read based assembly and annotation of a Drosophila simulans genome
Authors: Nouhaud Pierre 
Published: September 2018

AbstractLong-read sequencing technologies enable high-quality, contiguous genome assemblies. Here we used SMRT sequencing to assemble the genome of a Drosophila simulans strain originating from Madagascar, the ancestral range of the species. We generated 8 Gb of raw data (~50× coverage) with a mean read length of 6,410 bp, a NR50 of 9,125 bp and the longest subread at 49 kb. We benchmarked six different assemblers and merged the best two assemblies from Canu and Falcon. Our final assembly was 127.41 Mb with a N50 of 5.38 Mb and 305 contigs. We anchored more than 4 Mb of novel sequence to the major chromosome arms, and significantly improved the assembly of peri-centromeric and telomeric regions. Finally, we performed full-length transcript sequencing and used this data in conjunction with short-read RNAseq data to annotate 13,422 genes in the genome, improving the annotation in regions with complex, nested gene structures.
Influence of non-attendance on choices with varying complexity
Authors: Grebitus Carola , Roosen Jutta 
Published: September 2018

Purpose
The purpose of this research is to test how varying the numbers of attributes and alternatives affects the use of heuristics and selective information processing in discrete choice experiments (DCEs). The effects of visual attribute and alternative non-attendance (NA) on respondent choices are analyzed.

Design/methodology/approach
Two laboratory experiments that combined eye tracking and DCEs were conducted with 109 and 117 participants in the USA. The DCEs varied in task complexity by the number of product attributes and alternatives.

Findings
Results suggest that participants ignore both single attributes and entire alternatives. Increasing the number of alternatives significantly increased attribute NA. Including NA in choice modeling influenced results more in more complex DCEs.

Research limitations/implications
The current experiments did not test for choice overload. Future studies could investigate more complex designs. The choice environment affects decision-making. Future research could compare laboratory and field experiments.

Practical implications
Private and public sectors often use DCEs to determine consumer preference. Results suggest that DCEs with two alternatives are superior to DCEs with four alternatives because NA was lower in the two-alternative design.

Originality/value
This empirical research examined effects of attribute and alternative NA on choice modeling using eye tracking and DCEs with varying degrees of task complexity. Results suggest that accounting for NA reduces the risk of over- or understating the impact of attributes on choice, in that one avoids claiming significance for attributes that might not truly be preferred, and vice versa.
Predicting readers’ domain knowledge based on eye-tracking measures
Authors: Lu Quan , Zhang Jiyue , Chen Jing , Li Ji 
Published: September 2018

Purpose
This paper aims to examine the effect of domain knowledge on eye-tracking measures and predict readers’ domain knowledge from these measures in a navigational table of contents (N-TOC) system.

Design/methodology/approach
A controlled experiment of three reading tasks was conducted in an N-TOC system for 24 postgraduates of Wuhan University. Data including fixation duration, fixation count and inter-scanning transitions were collected and calculated. Participants’ domain knowledge was measured by pre-experiment questionnaires. Logistic regression analysis was leveraged to build the prediction model and the model’s performance was evaluated based on baseline model.

Findings
The results showed that novices spent significantly more time in fixating on text area than experts, because of the difficulty of understanding the information of text area. Total fixation duration on text area (TFD_T) was a significantly negative predictor of domain knowledge. The prediction performance of logistic regression model using eye-tracking measures was better than baseline model, with the accuracy, precision and F(β = 1) scores to be 0.71, 0.86, 0.79.

Originality/value
Little research has been reported in literature on investigation of domain knowledge effect on eye-tracking measures during reading and prediction of domain knowledge based on eye-tracking measures. Most studies focus on multimedia learning. With respect to the prediction of domain knowledge, only some studies are found in the field of information search. This paper makes a good contribution to the literature on the effect of domain knowledge on eye-tracking measures during N-TOC reading and predicting domain knowledge.
Corporate governance, globalization and firm performance in emerging economies
Authors: Saini Neha , Singhania Monica 
Published: September 2018

PurposeThe purpose of this paper is to examine relationship between corporate governance (CG) and firm performance for a set of 255 foreign-funded firms in the form of foreign direct investment (FDI) and private equity (PE). The authors employ a wide range of CG measures including board size, meetings, board gender and foreign ownership which are used as the proxy of globalisation and control variables like firm age, leverage, firm size and capital expenditure to arrive at a conclusion.Design/methodology/approachPanel data set of 255 (187 companies funded by foreign capital in the form of FDI, and 68 companies having foreign capital in the form PE) companies listed on Bombay Stock Exchange, for the period of eight years (2008–2015) are analysed by using static (fixed and random effects) and dynamic (generalised method of moments (GMM)) panel data specifications to examine the relationship among CG, globalisation and firm performance.FindingsThe empirical results of static model indicate the relationship between CG and performance of foreign firms, which are not very strong in India. This is due to the fact that most of the firms are not following the guidelines and regulations strictly in the initial period of sample years. Diversity in board is found as an important variable in accessing firm performance. And the authors also found that foreign firms are very particular about the implementation of CG norms. The results of GMM model highlight the interaction term of foreign ownership with governance indicators. CG is having a positive and significant impact over performance, inferring that higher foreign ownership (in the form of FDI and PE) in firm leading to positive effect on profitability.Practical implicationsThe investor’s preference of financing a unit is guided by the performance of a firm. Investors are more inclined towards high-performing firms, and hence higher profitability leads to higher inflow of capital. The result indicates that higher accounting and market performance may be achieved by good governance practices, in turn, leading to reduced agency costs. Countries with high governance scores attract more of foreign capital. Similar to the best governed countries, the companies having good governance practices attract more foreign inflows in the form of capital.Originality/valueWhile previous literature considered a single measurement framework in the form of a CG index, the authors tried to incorporate a range of CG indicators to study the effect of globalisation and CG on firm performance. The authors segregated foreign-owned funds into two parts, especially FDI and PE. This paper examined heterogeneity in the form of FDI-funded and PE-funded firms, as no prior literature is available which has evaluated different sets of foreign funds simultaneously on CG.
Comparison of performance measurement in different purchasing and supply management practices
Authors: Jääskeläinen Aki 
Published: September 2018

PurposeThe purpose of this paper is to compare the characteristics of performance measurement in cross-functional and supplier-oriented purchasing and supply management (PSM) practices. It clarifies the purposes and content of performance measurement in three PSM practices.Design/methodology/approachA multiple case study approach is utilized in this study. Each of the cases is related to a contemporary performance measurement development project during the period 2015–2016. Interviews are conducted to obtain a pre-understanding of the research questions at hand. The case studies continue as an action research including measurement development workshops.FindingsIdentification of causalities between measurement objects and the measurement value benefits are contemporary issues of performance measurement development in the PSM context. The findings indicate that the measurement of outputs and outcomes instead of inputs and measurement supporting supplier relationship management is increasingly important in the service context. Measurement purpose affects the characteristics of PSM performance measurement. This study identifies that more informal control and non-standard measures are needed in interactive PSM practices such as cross-functional integration.Research limitations/implicationsThe study provides practical examples of performance measurement in service purchasing, classifies the characteristics of performance measurement and highlights the need to extend the scope of measurement in the PSM context. The findings of the case studies reported support practitioners in developing performance measurement that satisfies contemporary managerial needs.Originality/valueEarlier studies are often not explicit with the purpose of performance measurement. This study contributes to the existing literature by linking performance measurement characteristics to the understanding of PSM practices and providing a wider overview of the varying characteristics of performance measurement supporting strategic purchasing of services.
The moderating effect of structural barriers on TQM-performance relationship in Egyptian service organizations
Authors: Khalaf Magdy A. , Salem Tamer S. Mohamed 
Published: September 2018

Purpose
This paper aims to empirically investigate how structural barriers affect the relationship between total quality management (TQM) practices implementation and organizational performance in service industries.

Design/methodology/approach
This research hypothesized the moderation effect of structural barriers on the relationship between TQM practices implementation and organizational performance. A questionnaire was adopted to collect data form 153 Egyptian service companies. Moderated regression analysis was used to test the study hypothesis.

Findings
The empirical analysis suggests that structural barriers partially moderate the relationship between TQM practices implementation and organizational performance. The analysis reveals that the effect of Quality Improvement, Process Improvement, External and Internal Relations and Employee Development – being as TQM dimensions – on performance is moderated by structural barriers. While the results provided insufficient evidence on the moderating effect of structural barriers on the relationship between both Performance Management – being as a TQM dimension – and performance.

Research limitations/implications
This research presents a new perspective for researches to understand the TQM–Performance relationship in the light of the contingency theory. However, the adopted sampling technique and the small sample size might limit the generalizability of the research findings.

Practical implications
This study provides useful insights for service organizations about the necessity of developing suitable structural platform for supporting their TQM efforts to boost their performance which, in turn, improves their competitiveness.

Originality/value
This research proposed and empirically validated how structural barriers play a significant role as moderators to the relationship between TQM implementation and organizational performance within service organizations context.
Fishing for whales: a segmentation model for social casinos
Authors: Barsky Jonathan 
Published: September 2018

PurposeThe purpose of this paper is to introduce a new customer segmentation model for the social casino industry. The key contribution of this model is the introduction of original psychographic/taste data, including a player emotions scale.Design/methodology/approachThe data for this research are based on player feedback from 22 countries, with evaluations of the top 100 social casino titles (apps). The new segmentation model splits the industry into distinct customer groups based on spending patterns, behavioral dimensions and attitudinal dimensions.FindingsThe results provide insight into game mechanics, social dynamics, player emotions, spend, price sensitivity, loyalty and other elements that impact monetization. Critical behaviors and preferences of social casino players that will help companies better understand and connect with their target customers are described.Originality/valueThis is the first study to develop a rigorous segmentation model of social casino games based on behavioral and psychographic data.
How store flyers design affect perceived variety of retailers’ assortment
Authors: Prediger Maciel , Huertas-Garcia Ruben , Gázquez-Abad Juan Carlos 
Published: September 2018

PurposeThe purpose of this paper is to investigate the relationship between several aspects of store flyers design (presence of a institutional slogan, type of product (national brand (NB) or store brand (SB)) featured on the cover page, the size of the flyer, number of featured NBs, type of brand (NB vs SB) on promotion, and price difference between the most expensive (NB) and the cheapest SB) and the consumer’s perceived variety of the retailer’s assortment, as a dimension of its global image.Design/methodology/approachA mixed laboratory experiment that combined a between-subjects experimental design and inter-subject conjoint analysis was conducted. A fictitious flyer from a fictitious supermarket was created that included both real NBs and fictitious SBs. In total, 12 scenarios (i.e. flyers) were tested using a sample of 406 participants.FindingsAnalysis suggests that longer flyers have the greatest influence on consumers’ perceived variety of a retailer’s assortment; a greater number of NBs in a category influenced consumers’ perceptions positively, and featuring SBs on the cover enhanced perceived variety. If a retailer features SBs on a flyer’s cover, longer flyers are recommended, and shorter flyers are recommended if NBs are featured on the cover. A retailer should promote its own brand only if the most expensive NBs are featured with SBs.Research limitations/implicationsThis study analyses a single aspect of consumers’ purchasing behaviors – variety of a retailer’s assortment. Future research should examine other variables related to consumers’ purchasing behaviors. This study uses an online context to test hypotheses, but many aspects of flyer design are physical. Future research should test current findings in offline contexts to compare results. Research should also explore moderation by consumer variables such as brand and store loyalty.Practical implicationsTo researchers, the authors offer improved understanding of how a flyer’s design affects the first stage of purchasing. To practitioners, results offer better understanding of positive returns on investment of store flyers, and to retailers, results offer a guide to creating and organizing flyers.Originality/valueThis study is first to assess how a flyer’s design influences a dimension of store image. Unlike extant research that examines store flyers using econometric models at the aggregate level, this study uses a laboratory experiment that combines a between-subjects design with conjoint analysis.