bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serial horizontal transfer of vitamin-biosynthetic genes enables the establishment of new nutritional symbionts in aphids’ di-symbiotic systems
Authors: Manzano-Marı́n Alejandro , Coeur d’acier Armelle , Clamens Anne-Laure , Orvain Céline , Cruaud Corinne , Barbe Valérie , Jousselin Emmanuelle 
Published: October 2019

Abstract
Many insects depend on obligate mutualistic bacteria to provide essential nutrients lacking from their diet. Most aphids, whose diet consists of phloem, rely on the bacterial endosymbiont Buchnera aphidicola to supply essential amino acids and B vitamins. However, in some aphid species, provision of these nutrients is partitioned between Buchnera and a younger bacterial partner, whose identity varies across aphid lineages. Little is known about the origin and the evolutionary stability of these di-symbiotic systems. It is also unclear whether the novel symbionts merely compensate for losses in Buchnera or carry new nutritional functions. Using whole-genome endosymbiont sequences of nine Cinara aphids that harbour an Erwinia-related symbiont to complement Buchnera, we show that the Erwinia association arose from a single event of symbiont lifestyle shift, from a free-living to an obligate intracellular one. This event resulted in drastic genome reduction, long-term genome stasis, and co-divergence with aphids. Fluorescence in situ hybridisation reveals that Erwinia inhabits its own bacteriocytes near Buchnera’s. Altogether these results depict a scenario for the establishment of Erwinia as an obligate symbiont that mirrors Buchnera’s. Additionally, we found that the Erwinia vitamin-biosynthetic genes not only compensate for Buchnera’s deficiencies, but also provide a new nutritional function; whose genes have been horizontally acquired from a Sodalis-related bacterium. A subset of these genes have been subsequently transferred to a new Hamiltonella co-obligate symbiont in one specific Cinara lineage. These results show that the establishment and dynamics of multi-partner endosymbioses can be mediated by lateral gene transfers between co-ocurring symbionts.
Land-use change has host-specific influences on avian gut microbiomes
Authors: San Juan Priscilla A , Hendershot J Nicholas , Daily Gretchen C , Fukami Tadashi 
Published: October 2019

Abstract
Human modification of the environment, particularly through land-use change, often reduces animal species diversity. However, the effect of land-use change on the gut microbiome of wildlife in human-dominated landscapes is not well understood despite its potential consequences for host health. We sought to quantify the effect of land-use change on wild bird gut microbiomes in a countryside landscape in Costa Rica, comprising a range of habitat types, ranging from primary and secondary forests to diversified and monoculture farms. We collected 280 fresh fecal samples from individuals belonging to six common species of saltator, thrushes, and warblers at 24 sites across this land-use gradient. Through 16S rRNA community profiling, we found that bacterial species composition responded to host species identity more strongly than to habitat type. In addition, we found evidence that habitat type affected microbial composition only for two of the six bird species. Our findings indicate that some host species and their microbiota may be more vulnerable to human disturbances than others.
Flavonoid-mediated immunomodulation of human macrophages involves key metabolites and metabolic pathways
Authors: Mendes Luís F. , Gaspar Vítor M. , Conde Tiago A. , Mano João F. , Duarte Iola F. 
Published: October 2019

AbstractThe ability of flavonoids to attenuate macrophage pro-inflammatory activity and to promote macrophage-mediated resolution of inflammation is still poorly understood at the biochemical level. In this study, we have employed NMR metabolomics to assess how therapeutically promising flavonoids (quercetin, naringenin and naringin) affect the metabolism of human macrophages, with a view to better understand their biological targets and activity. In vitro-cultured human macrophages were polarized to the pro-inflammatory M1 phenotype, through incubation with LPS + IFN-γ, and subsequently treated with each flavonoid. The metabolic signatures of pro-inflammatory polarization and of flavonoid incubations were then characterized and compared. The results showed that all flavonoids modulated the cells endometabolome with the strongest impact being observed for quercetin. Many of the flavonoid-induced metabolic variations were in the opposite sense to those elicited by pro-inflammatory stimulation. In particular, the metabolic processes proposed to reflect flavonoid-mediated immunomodulation of macrophages included the downregulation of glycolytic activity, observed for all flavonoids, anti-inflammatory reprogramming of the TCA cycle (mainly quercetin), increased antioxidant protection (quercetin), osmoregulation (naringin), and membrane modification (naringenin). This work revealed key metabolites and metabolic pathways involved in macrophage responses to quercetin, naringenin and naringin, providing novel insights into their immunomodulatory activity.
Homogeneous nucleation of corundum nanocrystallites by rapid heating of aluminum formate hydroxide-based precursor powder
Authors: Yoshida Michiyuki , Kato Yuta , Oumi Yasunori , Sakurada Osamu , Tanaka Makoto , Wada Masashi , Kitaoka Satoshi 
Published: October 2019

AbstractThe high density nucleation of α-Al2O3 nanocrystallites was observed by rapid heating of the aluminum formate hydroxide-based precursor powder at 1200 °C for 50 s. The nucleation of α-Al2O3 nanocrystallites with less 10 nm in size from high purity aluminum oxide matrix has not been observed to our knowledge. Based on the results of XRD and TEM, α-Al2O3 nanocrystallites nucleated from the amorphous phase which formed after thermal decomposition of the precursor powder. Subsequently, α-Al2O3 with hollow rod-like morphology formed through coalescence and growth of nanocrystallites after heating at 1200 °C for 1 min. The results obtained in this paper indicates a possible beneficial effect of the rapid heating and cooling of the aluminum formate hydroxide-based precursor powder on the precipitation of α-Al2O3 nanocrystallites.
Numerical Simulation of Mandible Bone Remodeling under Tooth Loading: A Parametric Study
Authors: Su Kangning , Yuan Li , Yang Jie , Du Jing 
Published: October 2019

AbstractBone adapts to the change of mechanical stimulus by bone remodeling activities. A number of numerical algorithms have been developed to model the adaptive bone remodeling under mechanical loads for orthopedic and dental applications. This paper examines the effects of several model parameters on the computed apparent bone density in mandible under normal chewing and biting forces. The density change rate was based on the strain energy density per unit mass. The algorithms used in this study containing an equilibrium zone (lazy zone) and saturated values of density change rate provides certain stability to result in convergence without discontinuous checkerboard patterns. The parametric study shows that when different boundary conditions were applied, the bone density distributions at convergence were very different, except in the vicinity of the applied loads. Compared with the effects of boundary conditions, the models are less sensitive to the choice of initial density values. Several models starting from different initial density values resulted in similar but not exactly the same bone density distribution at convergence. The results also show that higher reference value of mechanical stimulus resulted in lower average bone density at convergence. Moreover, the width of equilibrium zone did not substantially affect the average density at convergence. However, with increasing width, the areas with the highest and the lowest bone density areas were all reduced. The limitations of the models and challenges for future work were discussed for the better agreement between the computed results and the in vivo data.
Biopsy strategies for endoscopic screening of pre-malignant gastric lesions
Authors: Zhang Meng , Liu Shan , Hu Yue , Bao Hai-biao , Meng Li-na , Wang Xiao-teng , Xu Yi , Zhao Jing , Lu Bin 
Published: October 2019

AbstractOperative Link on Gastritis Assessment (OLGA) and Operative Link on Gastric Intestinal Metaplasia Assessment (OLGIM) were adopted to evaluate gastric risk stratification in five biopsy samples. This study aimed to evaluate the degree of gastric atrophy (GA) and intestinal metaplasia (IM) in five locations to detect a more representative biopsy sample in gastric cancer (GC) screening. Our study enrolled 368 patients and 5 biopsy pieces were acquired from them. Gastric risk stratification was calculated by OLGA and OLGIM staging system. The results revealed that the IM score in the incisura angularis was higher than that in the larger and lesser curvature of corpus mucosa (p = 0.037 andp = 0.030, respectively) and the IM score in the lesser curvature of antrum mucosa was higher than that in the incisura angularis mucosa (p = 0.018). IM is more frequently observed in the angulus region than in the lesser curvature of corpus in the mild degree (p = 0.004) and mild IM lesions in the lesser curvature of antrum were more frequently observed than in the incisura angularis mucosa (p = 0.004), Four biopsy pieces protocol (larger curvature and lesser curvature of the antrum, lesser curvature of the corpus and angulus) demonstrated accurate consistency (97.83% and 98.37%, respectively) with a Kendall’s tau-b of higher than 0.990, along with low misdiagnosis rates of OLGA and OLGIM (III + IV) (9.76% and 5.00%, respectively). Three biopsy pieces protocol (lesser curvature of the antrum and corpus, angulus biopsy) in OLGA and OLGIM staging system was close to the standard protocol (five biopsy specimens) with a consistency of 94.84% and 94.29% and has a Kendall’s tau-b higher than 0.950 and diagnostic omission rates of 9.76% and 5.00%, respectively, which was exactly the same with the four biopsy pieces protocol. Furthermore, it had the second-highest Youden index (0.902 and 0.950, respectively) and area under the ROC curve (0.992 and 0.996, respectively) for the screening of high-risk GC by OLGA and OLGIM stages. Thus, we recommended the angulus and the lesser curvature of antrum as a conventional biopsy and three biopsy pieces for further GC risk screening.
Textures of the tumour microenvironment
Authors: Di Martino Julie S , Mondal Chandrani , Bravo-Cordero Jose Javier 
Published: October 2019

Abstract
In this review, we present recent findings on the dynamic nature of the tumour microenvironment (TME) and how intravital microscopy studies have defined TME components in a spatiotemporal manner. Intravital microscopy has shed light into the nature of the TME, revealing structural details of both tumour cells and other TME co-habitants in vivo, how these cells communicate with each other, and how they are organized in three-dimensional space to orchestrate tumour growth, invasion, dissemination and metastasis. We will review different imaging tools, imaging reporters and fate-mapping strategies that have begun to uncover the complexity of the TME in vivo.
Speech Deterioration of Amyotrophic Lateral Sclerosis Before and After Diagnosis: A Case Study of a Newscaster
Authors: Watkins Emily , Thompson Austin , Kim Yunjung 
Published: October 2019

Purpose
This case study traced speech deterioration in an individual before and after the time of diagnosis of amyotrophic lateral sclerosis (ALS). Our participant was diagnosed with spinal-onset, familial ALS in 2017. The speaker's occupation, a professional newscaster for 37 years, allowed a retrospective examination of her speech during news segments over 37 months around the diagnosis, including prediagnosis.

Method
A total of 6 time points were selected to track auditory-perceptual and acoustic speech deterioration (2 years, 14 months, and 7 months prior to diagnosis; the month of diagnosis; and 7 months and 12 months after diagnosis). For perceptual ratings, 2 experts in motor speech disorders rated 17 speech dimensions on a 7-point scale. Four acoustic parameters were chosen for measurement: articulation rate, utterance duration, second formant frequency slope, and acoustic vowel space. Additionally, kinematic data were obtained from 1 time-point (8 months postdiagnosis) and descriptively compared to the movement of other individuals with ALS and to healthy speakers.

Results
As expected, both perceptual and acoustic results indicated a decline in the selected speech measures as the disease progressed. More interestingly, the measures showed a consistent curvilinear appearance in which the speech parameters exhibit an improvement until immediately before and around the diagnosis, followed by sudden, drastic deterioration. Kinematic results indicated a greater degree of movement and speed compared to healthy speakers, probably due to the speaker's occupation.

Conclusions
Based on the findings, the time around diagnosis is considered a critical period with respect to speech deterioration in ALS wherein a dynamic, increasing–decreasing pattern of changes occur. This finding appears to reflect the patient's compensatory strategies and the speech deficits associated with bulbar involvement.
Techno‐economic comparison of rooftop‐mounted PVs and small wind turbines: a case study for Brussels
Authors: Deltenre Quentin , De Troyer Tim , Runacres Mark C. 
Published: October 2019

As rooftops in cities are mostly underused, they have a large potential for decentralised electricity production. In that context, photovoltaic (PV) panels have proven to be an effective solution. Meanwhile, the market of small wind turbines is increasing, and some building owners have already installed one or more units on their roof. While the economic comparison between PV panels and wind turbines has already largely been addressed, in general, the space constraint of a rooftop has never been taken into account. In this work, the authors propose a methodology to compare the energy production and the return on investment both for rooftop‐mounted PV panels and wind turbines. The comparison is made for relatively tall buildings (≥60 m) with good wind conditions (≥5 m/s annual mean wind speed). Using a brute‐force approach, this study presents the results of the methodology applied to a case study: the Brussels Region. On tall rooftops, considering the space already taken by other installations and assuming an average wind speed of 5 m/s, small building‐mounted wind turbines are shown to produce more energy than PV panels. Nevertheless, their return on investment is always lower than that of the PV panels.
Update Lungenarterienembolie
Authors: Stimpfle Fabian , Geisler Tobias 
Published: October 2019

ZUSAMMENFASSUNGDie korrekte Behandlung der Lungenarterienembolie beginnt mit einer frühzeitigen multimodalen Einschätzung des Risikos unter Berücksichtigung der klinischen Vorhersagekraft, Laborparametern und bildmorphologischen Risikomerkmalen. Während die Lysetherapie hauptsächlich Patienten mit Schocksituation vorbehalten ist, kann sie bei ausgewählten stabilen Patienten mit intermediär hohem Risiko und vertretbarem Blutungsrisiko ebenfalls in Betracht gezogen werden. Im Niedrigrisikobereich ist eine ambulante Behandlung vertretbar. Neue Aspekte umfassen auch den Einsatz von kathetergestützen Reperfusionsmaßnahmen, sowie Algorithmen, um das Langzeitrezidivrisiko als auch die Entwicklung einer chronisch thrombembolischen pulmonalen Hypertonie frühzeitig zu erkennen. Der Vorteil der verlängerten Sekundärprophylaxe nach Lungenarterienembolie ist bei bestimmten Patienten durch Anwendung von klinischen Vorhersagemodellen und auch im Bereich der Tumorerkrankungen durch aktuelle Studiendaten belegt worden. Dieser Übersichtsartikel dient als Update unter Berücksichtigung der aktuellen Entwicklung und liefert Perspektiven über derzeit laufende Studien zur Klärung von offenen Fragen in der Behandlung der Lungenarterienembolie.
Intermediate Dose Low-Molecular-Weight Heparin for Thrombosis Prophylaxis: Systematic Review with Meta-Analysis and Trial Sequential Analysis
Authors: Eck Ruben J. , Bult Wouter , Wetterslev Jørn , Gans Reinold O.B. , Meijer Karina , Keus Frederik , van der Horst Iwan C.C. 
Published: October 2019

AbstractDifferent doses of low-molecular-weight heparin (LMWH) are registered and used for thrombosis prophylaxis. We assessed benefits and harms of thrombosis prophylaxis with a predefined intermediate-dose LMWH compared with placebo or no treatment in patients at risk of venous thromboembolism (VTE). We performed a systematic review with meta-analyses and trial sequential analyses (TSA) following The Cochrane Handbook for Systematic Reviews of Interventions. Medline, Cochrane CENTRAL, Web of Science, and Embase were searched up to December 2018. Trials were evaluated for risk of bias and quality of evidence was assessed following the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach. Seventy randomized trials with 34,046 patients were included. Eighteen (26%) had overall low risk of bias. There was a small statistically significant effect of LMWH on all-cause mortality (risk ratio [RR]: 0.96; TSA-adjusted confidence interval [TSA-adjusted CI]: 0.94–0.98) which disappeared in sensitivity analyses excluding ambulatory cancer patients (RR: 0.99; TSA-adjusted CI: 0.84–1.16). There was moderate-quality evidence for a statistically significant beneficial effect on symptomatic VTE (odds ratio [OR]: 0.59; TSA-adjusted CI: 0.53–0.67; number needed to treat [NNT]: 76; 95% CI: 60–106) and a statistically significant harmful effect on major bleeding (Peto OR: 1.66; TSA-adjusted CI: 1.31–2.10; number needed to harm [NNH]: 212; 95% CI: 142–393). There were no significant intervention effects on serious adverse events. The use of intermediate-dose LMWH for thrombosis prophylaxis compared with placebo or no treatment was associated with a small statistically significant reduction of all-cause mortality that disappeared in sensitivity analyses excluding trials that evaluated LMWH for anticancer treatment. Intermediate-dose LMWH provides benefits in terms of VTE prevention while it increases major bleeding.
Use of New Technologies in Implant-Based Breast Reconstruction
Authors: Jones Glyn E. , King Victor A. , Yoo Aran , Abu-Ghname Amjed , Rammos Charalambos K. 
Published: October 2019

AbstractOutcomes after mastectomy and prosthetic-based breast reconstruction have improved immensely since the development of the first tissue expander and breast implant in the 1960s. One major factor contributing to our improved outcomes over the past two decades is the increasing availability and improvement of perfusion assessment technology. Instrumental methods now exist which allow surgeons to assess tissue viability intraoperatively, and provide actionable, objective data that augments clinical assessment. In this article, the authors detail two commercially available, state-of-the-art technologies that surgeons may use to assist in mastectomy flap assessment and facilitate the reconstructive process.
Measurement of High-Sensitivity Cardiac Troponin in Pulmonary Embolism: Useful Test or a Clinical Distraction
Authors: Lippi Giuseppe , Favaloro Emmanuel J. , Kavsak Peter 
Published: October 2019

AbstractThe ability to predict death or other unfavorable outcomes after an acute pulmonary embolism (PE) is challenging, with current available risk score models having relatively unsatisfactory prognostic performance in this area. For example, the simplified pulmonary embolism severity index (sPESI), the most frequently used stratification tool, misclassifies a significant percentage of low- and high-risk patients. This gap in care, along with the increasing clinical availability of high-sensitivity cardiac troponin (hs-cTn) laboratory tests and the recent emphasis on detecting myocardial injury, may foster further evaluation of hs-cTn testing in patients with acute PE. Our analysis of the current scientific literature on hs-cTn in patients with acute PE identified that hs-cTn testing may provide valuable information for predicting future adverse outcomes and mortality, independently from baseline clinical risk assessment. Although the risk of an adverse event is indeed higher in patients with higher sPESI scores, cTns retain their prognostic value also in those at low risk, suggesting that a combination of hs-cTn with sPESI may provide an incremental value over assessment of either variable alone. Accordingly, the future development of updated risk stratification models, with the inclusion of laboratory tests such as hs-cTn, may represent an enhanced approach for risk stratification in patients with acute PE. Additional research, however, is needed to verify whether the combination of cTns, specifically as measured with hs-cTn assays, with other biomarkers may further improve the current capacity to efficiently manage patients with acute PE.
Venous Thromboembolism and Renal Impairment: Insights from the SWIss Venous ThromboEmbolism Registry (SWIVTER)
Authors: Spirk David , Sebastian Tim , Banyai Martin , Beer Jürg H. , Mazzolai Lucia , Baldi Thomas , Aujesky Drahomir , Hayoz Daniel , Engelberger Rolf P. , Kaeslin Thomas , Korte Wolfgang , Escher Robert , Husmann Marc , Mollet Annette , Szucs Thomas D. , Kucher Nils 
Published: October 2019

AbstractRenal impairment (RI) has increased substantially over the last decades. In the absence of data from confirmatory research, real-life data on anticoagulation treatment and clinical outcomes of venous thromboembolism (VTE) in patients with RI are needed. In the SWIss Venous ThromboEmbolism Registry (SWIVTER), 2,062 consecutive patients with objectively confirmed VTE were enrolled. In the present analysis, we compared characteristics, initial and maintenance anticoagulation, and adjusted 90-day clinical outcomes of those with (defined as estimated creatinine clearance < 30 mL/min) and without severe RI. Overall, 240 (12%) patients had severe RI; they were older, and more frequently had chronic and acute comorbidities. VTE severity was similar between patients with and without severe RI. Initial anticoagulation in patients with severe RI was more often performed with unfractionated heparin (44 vs. 24%), and less often with low-molecular-weight heparin (LMWH) (52 vs. 61%) and direct oral anticoagulants (DOACs; 4 vs. 12%). Maintenance anticoagulation in patients with severe RI was more frequently managed with vitamin K antagonists (70 vs. 60%) and less frequently with DOAC (12 vs. 21%). Severe RI was associated with increased risk of 90-day mortality (9.2 vs. 4.2%, hazard ratio [HR]: 2.27, 95% confidence interval [CI]: 1.41–3.65), but with similar risk of recurrent VTE (3.3 vs. 2.8%, HR: 1.19, 95% CI: 0.57–2.52) and major bleeding (2.1 vs. 2.0%, HR: 1.05, 95% CI: 0.41–2.68). In patients with severe RI, the use of LMWH versus any other treatment was associated with reduced mortality (HR: 0.37; 95% CI: 0.14–0.94; p = 0.036) and similar rate of major bleeding (HR: 0.59, 95% CI: 0.17–2.00; p = 0.39). Acute or chronic comorbidities rather than VTE severity or recurrence may explain increased early mortality in patients with severe RI. The higher rate of VTE recurrence, specifically fatal events, than major bleeding reinforces the need for effective anticoagulation in VTE patients with severe RI.