bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of duodenal perfusion by contrast‐enhanced ultrasonography in dogs with chronic inflammatory enteropathy and intestinal lymphoma
Authors: Nisa Khoirun , Lim Sue Yee , Shinohara Masayoshi , Osuga Tatsuyuki , Yokoyama Nozomu , Tamura Masahiro , Nagata Noriyuki , Sasaoka Kazuyoshi , Dermlim Angkhana , Leela‐Arporn Rommaneeya , Morita Tomoya , Sasaki Noboru , Morishita Keitaro , Nakamura Kensuke , Ohta Hiroshi , Takiguchi Mitsuyoshi 
Published: February 2019

BackgroundContrast‐enhanced ultrasonography (CEUS) can be used to evaluate intestinal perfusion in healthy dogs. It is helpful for diagnosing and monitoring inflammatory bowel disease in humans and could be useful for dogs with chronic intestinal diseases.ObjectivesTo examine duodenal perfusion in dogs with chronic inflammatory enteropathy (CIE) and intestinal lymphoma.AnimalsClient‐owned dogs with CIE (n = 26) or intestinal lymphoma (n = 7) and dogs with gastrointestinal signs but histopathologically normal duodenum (controls, n = 14).MethodsIn this cross‐sectional study, dogs with CIE were classified into remission (n = 16) and symptomatic (n = 10) groups based on clinical scores determined at the time of CEUS. The duodenum was scanned after IV injection of Sonazoid® (0.01 mL/kg). CEUS‐derived perfusion parameters, including time‐to‐peak, peak intensity (PI), area under the curve (AUC), and wash‐in and wash‐out rates were evaluated.ResultsThe PI was significantly higher in the symptomatic CIE group (median (range); 105.4 (89.3‐128.8) MPV) than in the control group (89.9 (68.5‐112.2) MPV). The AUC was significantly higher in the symptomatic CIE group (4847.9 (3824.3‐8462.8) MPV.sec) than in the control (3448.9 (1559.5‐4736.9) MPV.sec) and remission CIE (3862.3 (2094.5‐6899.0) MPV.sec) groups. The PI and clinical score were positively correlated in the CIE group. No significant differences in perfusion parameters were detected between the lymphoma and CIE groups or the lymphoma and control groups.Conclusions and Clinical ImportanceThe PI and AUC can detect duodenal inflammation and hence are potentially useful for excluding a diagnosis of CIE.
Effect of antimicrobials, salinity, and contamination by air on bacterial and fungal growth in cyprid cultures of Amphibalanus improvisus
Authors: Ochi Agostini Vanessa , Macedo Alexandre José , Muxagata Erik 
Published: February 2019

AbstractBarnacles are crustaceans of ecological and economic importance. Larvae of barnacles such as Amphibalanus improvisus are cultivated in laboratories in order to study their biology and ecology. However, the culturing of invertebrates promotes the growth of microorganisms, which can eventually kill the invertebrates. The use of antimicrobials combined with aseptic techniques can inhibit the growth of bacteria and fungi. The objective of this study was to evaluate the effect of antimicrobials on bacterioplankton and mycoplankton growth in cyprid cultures of A. improvisus at different salinities, with or without air exposure. For this, we evaluated the survival and settlement of A. improvisus cyprids and free‐living and biofilm bacterial inhibition in an antimicrobial medium exposed to different salinities (1, 15, and 25) and under uncovered and covered (PVC film) cultures. With the data obtained, we observed the absence of negative effects on the survival and settlement of A. improvisus and determined the antimicrobial treatment efficacy (using a combination of 0.025 g/L of penicillin G potassium, 0.08 g/L of streptomycin sulfate, 0.04 g/L of neomycin sulfate, and 0.005 g/L of nystatin) in inhibiting free‐living and biofilm microorganisms (bacteria and fungi). This treatment could be used to inhibit bacterial and fungal growth in marine cultures with a salinity above 25. The effectiveness of the treatment is considerably enhanced by covering the cultures to reduce contamination by air.
Very low‐energy and low‐energy formula diets: Effects on weight loss, obesity co‐morbidities and type 2 diabetes remission – an update on the evidence for their use in clinical practice
Authors: Brown A. , Leeds A. R. 
Published: February 2019

AbstractThe role of formula very low‐energy diets (VLEDs, <800 kcal/day) and low‐energy diets (LEDs, 800–1200 kcal/day) within clinical practice has regained attention over the last few years. Formula diets can achieve clinically significant weight reduction in the short‐term (3–5 months) and new evidence demonstrates that long‐term weight loss maintenance (up to 4 years) is achievable. Weight reductions of between 10% and 15% bodyweight have been reported, which is associated with clinically meaningful health outcomes in a number of obesity‐related co‐morbidities including type 2 diabetes (T2D), obstructive sleep apnoea and osteoarthritis. Recent evidence indicates that using a formula LED with a weight loss maintenance programme can help people with overweight or obesity and T2D achieve remission. Despite this, few healthcare professionals in the UK routinely use LEDs. Concerns about adherence, risk of precipitating eating disorders, safety, cost and long‐term efficacy may, in part, contribute to their under use. To help inform healthcare professionals on the use of formula diets within clinical practice, this review examines the current evidence for the use of VLEDs and LEDs for weight loss and weight loss maintenance, and in the treatment of obesity‐related co‐morbidities, including T2D, osteoarthritis, psoriasis, obstructive sleep apnoea and secondary coronary prevention, with a particular focus on T2D remission.
Belly dancer's dyskinesia in a 62‐year‐old man
Authors: Al‐Sibahee Essam , Fawzi Muhammed 
Published: February 2019

AbstractBelly dancer's dyskinesia is a rare condition characterized by involuntary, rhythmic movements of the abdominal wall. In this report, we present a case of 62‐year‐old man who presented to emergency department due to sudden onset of rhythmic, painless movements of the abdominal wall. He had a previous attack which resolved spontaneously. Examination and routine investigations were normal.
World Workshop on Oral Medicine VII: Burning mouth syndrome: A systematic review of disease definitions and diagnostic criteria utilized in randomized clinical trials
Authors: Ariyawardana Anura , Chmieliauskaite Milda , Farag Arwa M. , Albuquerque Rui , Forssell Heli , Nasri‐Heir Cibele , Klasser Gary D. , Sardella Andrea , Mignogna Michele D. , Ingram Mark , Carlson Charles R. , Miller Craig S. 
Published: February 2019

AbstractObjectiveTo conduct a systematic review analyzing disease definitions and diagnostic criteria used in randomized controlled trials (RCTs) involving burning mouth syndrome (BMS).MethodsA systematic search conducted in PubMed, Web of Science, PsycINFO, Cochrane Database/Cochrane Central, and Google Scholar that included RCTs on BMS published between 1994 and 2017 was performed.ResultsConsiderable variability in BMS disease definitions and diagnostic criteria used created substantial heterogeneity in the selection of participants and weakened the rigor of the 36 RCTs identified. The analyzed RCTs routinely under‐reported the methods used to rule in or out study participants and the number of individuals excluded from BMS RCTs.ConclusionsOur findings indicate that a large proportion of participants enrolled in these studies may have had an underlying condition that could have explained their BMS symptoms. Thus, outcomes of therapeutic interventions from these BMS RCTs should be interpreted with caution due to heterogeneous disease definitions and diagnostic criteria. In order to improve the quality of clinical trials, future research should focus on establishing consensus for a single definition of BMS that includes specific inclusion and exclusion criteria that should be used to select study participants for clinical trials.
Novel PHOX2B mutations in congenital central hypoventilation syndrome
Authors: Sasaki Ayako , Kishikawa Yumiko , Imaji Reisuke , Fukushima Yu , Nakamura Yukiko , Nishimura Yutaka , Yamada Megumi , Mino Yoichi , Mitsui Tetsuo , Hayasaka Kiyoshi 
Published: February 2019

AbstractBackgroundCongenital central hypoventilation syndrome (CCHS) is caused by mutation of paird‐like homeobox 2B (PHOX2B). Approximately 90% of patients were found to carry polyalanine repeat expansion mutation (PARM), and the remaining 10% had non‐PARM (NPARM). In PARM, the length of the polyalanine expansion correlates with clinical disease severity. Most patients with NPARM have hypoventilation symptoms in the neonatal period and complications of Hirschsprung disease, dysregulation of autonomic nervous system, and tumors of neural crest origin. Data on the genotype–phenotype association may contribute to the clinical management of the disease.MethodsWe studied the genetic background of Japanese CCHS patients according to PHOX2B sequencing.ResultsOf 133 Japanese CCHS patients we identified 12 patients carrying 11 different NPARM (approx. 9% of the patients) and described the clinical manifestations in seven of them with the following novel mutations: c.941‐945del5, c.678_693dup16, c.609_616del8, c.620_633del14, c.663_711del 49, c.448C>G and c.944G>C. All patients had hypoventilation in the neonatal period and also had Hirschsprung disease, with the exception of two patients carrying c.620_633del14 and c.663_711del49 mutations. The patient carrying the c.609_616del8 mutation also had a benign mediastinal tumor.ConclusionMost patients carrying NPARM had severe symptoms with frequent complications, as in previous reports, and should be carefully monitored for various complications, including neural crest‐derived tumor.
Gastrostomy tubes appear to be safe following pediatric orthotopic heart transplant
Authors: Vavolizza Rick D. , Grabski David F. , Levin Daniel E. , Gander Jeffrey W. 
Published: February 2019

AbstractBackgroundGastrostomy tube (GT) placement is a common pediatric surgical procedure typically indicated for oral aversion. This may develop in patients with congenital heart disease (CHD) who require an orthotopic heart transplant (OHT). The safety profile of GT placement in OHT patients who are immunosuppressed is unknown. Given the potential increased risk of wound site complications on a patient receiving immunosuppression, we sought to determine the safety profile of GT placement in pediatric patients with OHT.Materials and MethodsWe performed a retrospective case series of all pediatric OHT recipients who subsequently underwent GT placement from January 1, 2009, to August 1, 2018, at the University of Virginia Children's Hospital. Major GT complications of wound breakdown, wound infection, peristomal GT leakage, ileus, or persistent emesis, and minor GT complication including the presence of granulation tissue are reported.ResultsSix patients who had a pediatric OHT subsequently underwent GT placement over the study period. There were no major 30‐day or 90‐day GT complications. One patient had excessive granulation tissue at their GT site. There were no accounts of acute kidney injury, urinary tract infection, sepsis, or pneumonia.ConclusionGastrostomy tube (GT) placement appears to be safe in pediatric OHT patients who are on immunosuppressive medications and unable to feed orally. This is the first study documenting the safety profile of GTs in pediatric OHT patients and may aid clinicians to make decisions regarding this intervention.
Antipsychotic drug use and favourable natures of emotional functions in patients with dementia
Authors: Fujii Masahiko , Butler James P. , Sasaki Hidetada 
Published: February 2019

AimTo characterise the effects of antipsychotics on brain functions of patients with behavioural psychological symptoms of dementia (BPSD).MethodsStudies were performed during 6 weeks of intervention with either a nonantipsychotic drug (group A, n = 38) or an antipsychotic drug (group B, n = 10).ResultsNeuropsychiatric Inventory scores were significantly decreased in both groups. Scores on the Mini‐Mental State Examination did not change with intervention. By contrast, favourable natures of emotional functions, scored using the Delightful Emotional Index of 10 items, were significantly lower in the antipsychotic drug treatment group B relative to scores in the nonantipsychotic drug treatment group A.ConclusionsAntipsychotics reduce favourite emotions as well as BPSD, and this should be considered in prescribing medications for patients with dementia.
Activin‐A receptor expression patterns in prepubertal goat oocytes and derived embryos
Authors: Morató Roser , Hammami Sondes , Paramio Maria Teresa , Izquierdo Dolors 
Published: February 2019

ContentsThis study examines the presence of activin IIA and IIB receptors (ActR‐IIA and ActR‐IIB) by Western blotting and immunocytochemistry in immature and IVM‐oocytes, 2 to 8‐cells embryos and blastocysts from prepubertal goats. Western blotting revealed that activin receptors are synthesized during oocyte maturation and embryo development. In the immunocytochemistry experiments, no immunostaining for either receptor was detected in oocytes while both receptors were immunolabelled in all the cells of cleaved embryos. In blastocysts, while ActR‐IIA expression appeared evenly distributed in the two cell lineages, inner cell mass and trophectoderm, the ActR‐IIB immunosignal was restricted mainly to the inner cell mass. Our findings reveal the presence of activin type II receptors (ActR‐IIA and ActR‐IIB) in in vitro matured prepubertal goat oocytes and blastocyst‐stage embryos. The expression of these receptors could be a key factor in understanding differences between competent and incompetent oocytes.
The use of human and bovine commercial media for oocyte maturation and embryo development in the domestic cat (Felis catus)
Authors: Prochowska Sylwia , Nizanski Wojciech , Partyka Agnieszka , Kochan Joanna , Młodawska Wiesława , Nowak Agnieszka , Skotnicki Józef , Grega Teresa , Pałys Marcin 
Published: February 2019

ContentsThe aim of this study was to examine the suitability of commercial media designed for humans and cattle for oocyte maturation and embryo culture in the domestic cat. In Exp. I, feline oocytes collected ex vivo were subjected to in vitro maturation in a laboratory‐made culture medium (based on M199) or a commercial medium designed for cattle cells (BO‐IVM®). In Exp. II, ICSI‐derived feline embryos were cultured for 7 days in a commercial human (Continuous Single Culture®) or bovine (BO‐EC®) cell medium. The rates of cleavage, morula and blastocyst formation were evaluated at 24 hr, 6 days and 7 days after ICSI, respectively, and compared between experimental groups. At the end of culture, embryos were assessed for viability and apoptotic changes. In Exp. I, no statistically significant difference in oocyte maturation outcome between laboratory‐made (52.7%) and commercial media (58.9%) was observed. However, the use of a commercial medium prepared for use with bovine cells resulted in a significantly lower variance of the maturation rate. In Exp. II, no statistically significant differences between two commercial media were observed for cleavage (67.5% and 64.5%), morula (39.3% and 47.1%) and blastocyst rates (25.0% and 19.6%), as well as for the percentage of late apoptotic blastomeres. Morulae cultured in medium marketed for humans exhibited significantly more early apoptotic (43.2 ± 31.2% vs. 23.4 ± 23.2%) and necrotic (60.6 ± 47.6% vs. 29.4 ± 22.6%) blastomeres. In conclusion, both commercial media tested are suitable for in vitro oocyte maturation and embryo culture procedures in cats. It is remarkable that a culture medium designed for use in cattle for in vitro maturation of cat oocytes provides more reproducible results.
Associations between FCGR polymorphisms and immune thrombocytopenia: A meta‐analysis
Authors: Li Gang , Gao Lan , Ma Rufei , Tian Wenqian , Mingzhi Ling 
Published: February 2019

AbstractSeveral studies already explored associations between Fc gamma receptor (FCGR) polymorphisms and immune thrombocytopenia (ITP), but the results of these studies were not consistent. Consequently, we conducted a meta‐analysis of relevant studies to better analyse the effects of FCGR polymorphisms on individual susceptibility to ITP. PubMed, Web of Science, Embase and CNKI were searched for eligible studies. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated. Totally 17 studies were eligible for analyses (1200 cases and 1723 controls). Significant associations with ITP were observed for FCGR3A F158V polymorphism in dominant (P < 0.0001, OR = 0.47, 95% CI 0.39‐0.57), recessive (P < 0.0001, OR = 2.03, 95% CI 1.58‐2.61), overdominant (P < 0.0001, OR = 1.42, 95% CI 1.19‐1.69) and allele (P < 0.0001, OR = 0.58, 95% CI 0.51‐0.65) models in overall analyses. But we did not observe any significant associations with ITP for FCGR2A H131R and FCGR2B I232T polymorphisms in overall analyses. Subgroup analyses by ethnicity yielded similar positive results for FCGR3A F158V polymorphism in both Asians and Caucasians. Furthermore, subgroup analyses by type of disease revealed that FCGR2A H131R polymorphism was significantly associated with childhood‐onset ITP, and FCGR3A F158V polymorphism was significantly associated with both childhood‐onset and adult‐onset ITP. In summary, our findings suggested that FCGR2A H131R polymorphism may serve as a potential genetic biomarker of childhood‐onset ITP, while FCGR3A F158V polymorphism may serve as a potential genetic biomarker of both childhood‐onset and adult‐onset ITP.
Comprehensive mass spectrometry‐guided phenotyping of plant specialized metabolites reveals metabolic diversity in the cosmopolitan plant family Rhamnaceae
Authors: Kang Kyo Bin , Ernst Madeleine , van der Hooft Justin J. J. , da Silva Ricardo R. , Park Junha , Medema Marnix H. , Sung Sang Hyun , Dorrestein Pieter C. 
Published: February 2019

SummaryPlants produce a myriad of specialized metabolites to overcome their sessile habit and combat biotic as well as abiotic stresses. Evolution has shaped the diversity of specialized metabolites, which then drives many other aspects of plant biodiversity. However, until recently, large‐scale studies investigating the diversity of specialized metabolites in an evolutionary context have been limited by the impossibility of identifying chemical structures of hundreds to thousands of compounds in a time‐feasible manner. Here we introduce a workflow for large‐scale, semi‐automated annotation of specialized metabolites and apply it to over 1000 metabolites of the cosmopolitan plant family Rhamnaceae. We enhance the putative annotation coverage dramatically, from 2.5% based on spectral library matches alone to 42.6% of total MS/MS molecular features, extending annotations from well‐known plant compound classes into dark plant metabolomics. To gain insights into substructural diversity within this plant family, we also extract patterns of co‐occurring fragments and neutral losses, so‐called Mass2Motifs, from the dataset; for example, only the Ziziphoid clade developed the triterpenoid biosynthetic pathway, whereas the Rhamnoid clade predominantly developed diversity in flavonoid glycosides, including 7‐O‐methyltransferase activity. Our workflow provides the foundations for the automated, high‐throughput chemical identification of massive metabolite spaces, and we expect it to revolutionize our understanding of plant chemoevolutionary mechanisms.
Production of RBC autoantibody mimicking anti‐D specificity following transfusion in a patient with weak D Type 15
Authors: Takeuchi‐Baba Chikako , Ito Shoichi , Kinjo Rie , Miyagi Hitomi , Yasuda Hiroyasu , Ogasawara Kenichi , Ohto Hitoshi 
Published: February 2019

BACKGROUNDWeak D‐type RBCs have fewer D epitopes, but it remains unclear whether individuals with certain types of weak D produce alloanti‐D directed at D epitopes absent from the RBCs, and whether it is an alloantibody or an autoantibody. We report the first case of a patient with a weak D Type 15 who produced autoantibodies mimicking alloanti‐D.CASE REPORTA 52‐year‐old Japanese male with weak D developed anti‐D 3 months after transfusion of D‐negative and ‐positive RBCs, and the antibody persisted for 24 months with a consistently negative direct antiglobulin test. Eluates from the patient's RBCs demonstrated anti‐D specificity. The recipient did not exhibit any signs of delayed hemolytic transfusion reaction. As his anti‐D was removed by the different adsorbing cells of weak D Type 15 and autologous as well as D positive, D negative, weak D Type 24, and partial DVa, it was thought to be an autoantibody mimicking anti‐D rather than an alloantibody. The patient's RBCs reacted weakly with the 13 anti‐D reagents used in the study. Polymerase chain reaction and nucleotide sequencing revealed that the patient had an RHD genotype of RHD*01N.01/RHD*15.CONCLUSIONAnti‐D, produced in a patient with weak D Type 15 after transfusion, was found to be mimicking autoanti‐D. Alloanti‐D was excluded by an adsorption study with different RBC types.
Evaluation of the i‐STAT analyzer for determination of ionized calcium concentrations in bovine blood
Authors: Yilmaz Oznur , Karapinar Tolga 
Published: February 2019

AbstractBackgroundInformation regarding the performance of the i‐STAT, a point‐of‐care analyzer, to determine ionized calcium (iCa2+) concentration in bovine blood is limited.ObjectiveThe primary goal of this study was to evaluate the agreement of the i‐STAT against a traditional bench‐top blood gas analyzer (Radiometer ABL800 FLEX) in the measurement of iCa2+in the blood of cattle with various disorders.MethodsIn this study, 121 cattle with various diseases were used. Venous blood samples were run on the i‐STAT and the Radiometer ABL800 FLEX for determination of blood iCa2+concentrations. Data were subjected to Passing‐Bablok regression and Bland‐Altman plots. The Radiometer ABL800 FLEX was the reference method and the i‐STAT was the test method. The i‐STAT performance was evaluated at two different cutoff values for iCa2+concentrations (<1.00 and <1.18 mmol/L) using the receiver operating characteristic curve.ResultsThe Passing‐Bablok regression equation that fit the iCa2+concentration as measured with the i‐STAT (Y) and the Radiometer ABL800 FLEX (X), wasY = −0.108276 [−0.15/−0.06] + 1.103448 [1.06/1.14] × X, with a residual deviation of 0.02. The deviation from the identity line was not significant (P = 0.64). The Bland‐Altman difference plot revealed no bias between the i‐STAT and the Radiometer ABL 800 FLEX. The i‐STAT had a sensitivity of 100% (95% CI, 87.7‐100.0) and 94.8% (95% CI, 87.2‐98.6) at the iCa2+concentration cutoff values of <1.00 and <1.18 mmol/L, respectively.ConclusionsiCa2+concentrations in bovine blood measured with the i‐STAT agreed with those of the Radiometer ABL800 FLEX. In particular, the i‐STAT predicted hypocalcemia with a high sensitivity.