bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anosognosia of financial ability in mild cognitive impairment
Authors: Gerstenecker Adam , Martin Roy C. , Triebel Kristen L. , Marson Daniel C. 
Published: April 2019

ObjectivesAlthough financial ability has been well‐studied in mild cognitive impairment (MCI) and Alzheimer's disease (AD) using performance‐based financial capacity assessment instruments, research is limited investigating everyday financial problems and declines in persons with AD and MCI and the insight of people with MCI to recognize that financial capacity declines are occurring. To address this gap in the research, we investigated everyday financial activities and skills in a sample of older adults representing the dementia spectrum.MethodsParticipants were 186 older adults in three diagnostic classifications: cognitively healthy, MCI likely due to AD, and mild AD dementia. Everyday financial ability was assessed using the Current Financial Activities Report (CFAR). The CFAR is a standardized report‐based measure which elicits participant and study partner ratings about a participant's everyday financial abilities.ResultsResults showed that both CFAR self‐ and study partner‐report distinguished diagnostic groups on key financial capacity variables in a pattern consistent with level of clinical pathology. Study partner‐report indicated higher levels of financial skill difficulties in study participants than did the self‐report of the same study participants. Study partner‐ratings were more highly correlated with participant scores on a performance‐based measure of financial capacity than were participant self‐ratings. Results also showed that loss of awareness of financial decline is emerging at the MCI stage of AD.ConclusionsPeople with MCI represent a group of older adults at particular risk for financial missteps and—similar to people with AD—are in need of supervision of their financial skills and activities.
Induction of cell fusion/apoptosis in anaplastic thyroid carcinoma in orthotopic mouse model by urokinase‐specific oncolytic Sendai virus
Authors: Miyagawa Yoshihiro , Araki Koji , Yamashita Taku , Tanaka Shingo , Tanaka Yuya , Tomifuji Masayuki , Ueda Yasuji , Yonemitsu Yoshikazu , Shimada Hideaki , Shiotani Akihiro 
Published: April 2019

AbstractBackgroundThis study was designed to assess the therapeutic effect of urokinase‐targeted recombinant oncolytic Sendai virus, termed “BioKnife,” on anaplastic thyroid carcinoma (ATC).MethodsUrokinase activity was investigated in human ATC cell lines, and BioKnife cytotoxicity against the cell lines was evaluated in vitro. Orthotopic mouse models of ATC were treated with three intratumoral injections of BioKnife, control virus, or phosphate‐buffered saline (PBS) and were observed daily until >20% weight loss occurred.ResultsAll three ATC cell lines showed a high level of urokinase activity. BioKnife induced urokinase‐dependent cell fusion and cytotoxicity in all cell lines. Orthotopic models treated with BioKnife showed significantly prolonged survival compared with models treated with control virus or PBS (BioKnife 41.6 ± 15.0, control virus 17.0 ± 2.9, PBS 17.7 ± 6.3 days).ConclusionsBioKnife exerted therapeutic effects in orthotopic ATC mouse models. Thus, BioKnife represents a possible treatment option for ATC.
The impact of mentoring quality on protégés' organization‐based self‐esteem and proactive behavior: The moderating role of traditionality
Authors: Wu Xiangfan , Lyu Yijing , Kwan Ho Kwong , Zhai Haiyan 
Published: April 2019

AbstractBased on self‐consistency theory, this study examined the relationship between mentoring quality as perceived by protégés and protégés' proactive behavior. It focused on the mediating role of organization‐based self‐esteem (OBSE) and the moderating role of traditionality. To examine these relationships, we administrated three‐wave surveys to 237 subordinate–supervisor dyads in a construction enterprise. The results of hierarchical linear modeling demonstrated that (a) mentoring quality and proactive behavior had a positive relationship; (b) OBSE mediated this relationship; and (c) traditionality strengthened both the relationship between mentoring quality and OBSE and the indirect effect of mentoring quality on proactive behavior via OBSE. Our findings have theoretical and practical implications for research on mentoring and proactive behavior.
Effects of RNA binding protein QKI on pancreatic cancer ductal epithelial cells and surrounding activation fibroblasts
Authors: Chu Liang , Hu Yuqin , Jiang Ying‐Hao , Xu Chengming , Liu Wen‐Chao , Lu Zi‐fan 
Published: April 2019

AbstractTo determine the correlation between QKI and pancreatic cancer tissues, the QKI expression of pancreatic cancer cells and fibroblasts in the tumor‐surrounding microenvironment were detected. Then, QKI overexpression and interference with QKI short hairpin RNA in LX‐2 (a fibroblast cell line) were established in vitro. Meanwhile, to observe the cell proliferation, invasion, migration, and other changes, QKI, and related epithelial‐mesenchymal transition (EMT) molecules were detected by a polymerase chain reaction and Western blot analysis. In addition, an in vivo tumorigenicity test in node mice was performed to confirm whether QKI expression can promote the proliferation, invasion, and metastasis of pancreatic cancer ductal epithelial cells. Finally, the autophagy levels of fibroblasts with QKI overexpression were observed by electron microscopy to further explore the QKI pathogenic mechanism. It was found that cell proliferation, invasion, migration, and EMT‐related markers were increased in QKI‐overexpressed fibroblasts LX‐2. Furthermore, in vivo, liver and peritoneal metastasis decreased overall survival rate and increasing autophagy levels in QKI‐overexpressing nude mice were observed. Meanwhile, knock down QKI with small interfering RNA can reverse all the above effects. QKI can promote the proliferation, metastasis, and invasion of pancreatic cancer through activating fibroblasts surrounding pancreatic cancer and accelerating EMT and increasing the autophagy in pancreatic cancer. QKI may become a potential target for the treatment of pancreatic cancer.
Hypobaric hypoxia regulates iron metabolism in rats
Authors: Li Yaru , Zhou Yue , Zhang Dong , Wu Wen‐Yue , Kang Xiaoxuan , Wu Qiong , Wang Peina , Liu Xiaopeng , Gao Guofen , Zhou Yaru , Wang Guangyou , Chang Yan‐Zhong 
Published: April 2019

AbstractIntermittent hypobaric hypoxia can produce a protective effect on both the nervous system and non‐nervous system tissues. Intermittent hypobaric hypoxia preconditioning has been shown to protect rats from cardiac ischemia‐reperfusion injury by decreasing cardiac iron levels and reactive oxygen species (ROS) production, thereby decreasing oxidative stress to achieve protection. However, the specific mechanism underlying the protective effect of hypobaric hypoxia is unclear. To shed light on this phenomenon, we subjected Sprague‐Dawley rats to hypobaric hypoxic preconditioning (8 hours/day). The treatment was continued for 4 weeks. We then measured the iron content in the heart, liver, spleen, and kidney. The iron levels in all of the assessed tissues decreased significantly after hypobaric hypoxia treatment, corroborating previous results that hypobaric hypoxia may produce its protective effect by decreasing ROS production by limiting the levels of catalytic iron in the tissue. We next assessed the expression levels of several proteins involved in iron metabolism (transferrin receptor, L‐ferritin, and ferroportin1 [FPN1]). The increased transferrin receptor and decreased L‐ferritin levels after hypobaric hypoxia were indicative of a low‐iron phenotype, while FPN1 levels remained unchanged. We also examined hepcidin, transmembrane serine proteases 6 (TMPRSS6), erythroferrone (ERFE), and erythropoietin (EPO) levels, all of which play a role in the regulation of systemic iron metabolism. The expression of hepcidin decreased significantly after hypobaric hypoxia treatment, whereas the expression of TMPRSS6 and ERFE and EPO increased sharply. Finally, we measured serum iron and total iron binding capacity in the serum, as well as red blood cell count, mean corpuscular volume, hematocrit, red blood cell distribution width SD, and red blood cell distribution width CV. As expected, all of these values increased after the hypobaric hypoxia treatment. Taken together, our results show that hypobaric hypoxia can stimulate erythropoiesis, which systemically draws iron away from nonhematopoietic tissue through decreased hepcidin levels.
Thermosensitive hybrid hyaluronan/p(HPMAm‐lac)‐PEG hydrogels enhance cartilage regeneration in a mouse model of osteoarthritis
Authors: Agas Dimitrios , Laus Fulvio , Lacava Giovanna , Marchegiani Andrea , Deng Siyuan , Magnoni Federico , Silva Guilherme Gusmão , Di Martino Piera , Sabbieti Maria Giovanna , Censi Roberta 
Published: April 2019

AbstractOsteoarthritis (OA), due to cartilage degeneration, is one of the leading causes of disability worldwide. Currently, there are not efficacious therapies to reverse cartilage degeneration. In this study we evaluated the potential of hybrid hydrogels, composed of a biodegradable and thermosensitive triblock copolymer cross‐linked via Michael addition to thiolated hyaluronic acid, in contrasting inflammatory processes underlying OA. Hydrogels composed of different w/w % concentrations of hyaluronan were investigated for their degradation behavior and capacity to release the polysaccharide in a sustained fashion. It was found that hyaluronic acid was controllably released during network degradation with a zero‐order release kinetics, and the release rate depended on cross‐link density and degradation kinetics of the hydrogels. When locally administered in vivo in an OA mouse model, the hydrogels demonstrated the ability to restore, to some extent, bone remineralization, proteoglycan production, levels of Sox‐9 and Runx‐2. Furthermore, the downregulation of proinflammatory mediators, such as TNF‐α, NFkB, and RANKL and proinflammatory cytokines was observed. In summary, the investigated hydrogel technology represents an ideal candidate for the potential encapsulation and release of drugs relevant in the field of OA. In this context, the hydrogel matrix could act in synergy with the drug, in reversing phenomena of inflammation, cartilage disruption, and bone demineralization associated with OA.
The effect of fludrocortisone on the uterine receptivity partially mediated by ERK1/2‐mTOR pathway
Authors: Hesam Shariati Mohammad Bakhtiar , Seghinsara Abbas Majdi , Shokrzadeh Naser , Niknafs Behrooz 
Published: April 2019

AbstractImplantation of embryos needs endometrial receptivity. Mineralocorticoids is one of the causes influencing the implantation window. This study targeted to evaluation fludrocortisone different properties on endometrial receptivity. The objective of this study was to assess whether treatment with fludrocortisone could impact the expression of diverse genes and proteins that are involved in uterine receptivity in mice. In this study, 40 female adult BALB/c mice were used. The samples were allocated to four groups of ten. Control group (C) received: vehicle; fludrocortisone group (FCA): received 1.5 mg/kg fludrocortisone; PP242 group (PP242): received 30 mg/kg PP242; fludrocortisone+PP242 group (FCA+PP242): received fludrocortisone and PP242. Mice were killed on window implantation day after mating and confirmed pregnancy. The endometrial epithelium of mouse was collected to assess mRNA expression of leukemia inhibitory factor (LIF), mucin‐1 (MUC1), heparin‐binding epidermal growth factor (HB‐EGF), (Msx.1), miRNA Let‐7a, and miRNA 223‐3p as well as protein expression of extracellular signal‐regulated kinase 1/2 (ERK1/2), mammalian target of rapamycin (mTOR), and eukaryotic translation initiation factor 4E‐binding protein 1 (4E‐BP1) in the uterine using real‐time PCR and western blot, respectively. In comparison with the control group, fludrocortisone administration upregulated the expression of LIF, HB‐EGF, Msx.1, miRNA Let‐7a, ERK1/2, and mTOR in the epithelial endometrium. The PP242‐treated group demonstrated a significant rise in the expression of MUC1, miRNA 223‐3p and a remarkable decline in ERK1/2 and p‐4E‐BP1 levels in comparison with the control group. Combination therapy of (FCA+PP242) resulted in a remarkable rise in LIF, Msx‐1, HB‐EGF, ERK1/2, and mTOR levels, in comparison with the PP242 group. Furthermore, combination therapy of (FCA+PP242) downregulated the expression of MUC1 in comparison with the PP242‐treated group. According to the results, fludrocortisone affected uterine receptivity possibly by means of modulating the expression of genes involved in the uterine receptivity and activation of the ERK1/2‐mTOR pathway.
Involvement of aberrant regulation of epigenetic mechanisms in the pathogenesis of Parkinson's disease and epigenetic‐based therapies
Authors: Renani Pedram G. , Taheri Forogh , Rostami Daryoush , Farahani Najmeh , Abdolkarimi Hamed , Abdollahi Elahe , Taghizadeh Eskandar , Gheibi Hayat Seyed Mohammad 
Published: April 2019

AbstractParkinson's disease (PD) is known as a progressive neurodegenerative disorder associated with the reduction of dopamine‐secreting neurons and the formation of Lewy bodies in the substantia nigra and basal ganglia routes. Aging, as well as environmental and genetic factors, are considered as disease risk factors that can make PD as a complex one. Epigenetics means studying heritable changes in gene expression or function, without altering the underlying DNA sequence. Multiple studies have shown the association of epigenetic variations with onset or progression of various types of diseases. DNA methylation, posttranslational modifications of histones and presence of microRNA (miRNA) are among epigenetic processes involved in regulating pathways related to the development of PD. Unlike genetic mutations, most epigenetic variations may be reversible or preventable. Therefore, the return of aberrant epigenetic events in different cells is a growing therapeutic approach to treatment or prevention. Currently, there are several methods for treating PD patients, the most important of which are drug therapies. However, detection of genes and epigenetic mechanisms involved in the disease can develop appropriate diagnosis and treatment of the disease before the onset of disabilities and resulting complications. The main purpose of this study was to review the most important epigenetic molecular mechanisms, epigenetic variations in PD, and epigenetic‐based therapies.
One‐pot conversion of carbohydrates into γ‐valerolactone under the coordination of heteropoly acid based ionic liquid and Ru/ZrO2 in water media
Authors: Ren Hui‐Fang , Zhu Dan , Li Ji‐Fan , Liu Chun‐Ling , Yang Rong‐Zhen , Dong Wen‐Sheng 
Published: April 2019

AbstractBACKGROUNDThe chemical catalytic conversion of carbohydrates into γ‐valerolactone (GVL) is a sustainable alternative to the petrochemical process starting from the depleting fossil resources. Direct conversion of carbohydrates (fructose, glucose, starch and cellulose) into GVL was achieved via the combined heteropoly acid based SO3H‐functionalized ionic liquid (HAIL) and supported ruthenium (Ru) catalyst system in one pot and in water media.RESULTSA 63% GVL yield (based on fructose) was obtained under the catalysis of 1‐methyl‐3‐(3‐sulfopropylimidazolium)silicotungstate ([MIMPS]4SiW) and Ru/ZrO2 at 180 °C, first in 0.1 MPa nitrogen (N2) for 3 h (dehydration), and then in 4 MPa hydrogen (H2) for 10 h (hydrogenation). Under the same reaction conditions, the GVL yields from glucose, starch and cellulose were 68%, 60% and 60%, respectively. The recyclability of the catalytic system consisting of [MIMPS]4SiW and Ru/ZrO2 was investigated in four runs. The activity for [MIMPS]4SiW did not change after use. Partial deactivation of Ru/ZrO2 was observed, which was ascribed to the severe carbon deposition.CONCLUSIONSIn the present method, HAIL and Ru/ZrO2 catalysts were loaded together. No separation and/or neutralization step reported in the literature was needed and only an atmosphere change (from N2 to H2) step was involved. Therefore, we provide a promising method for efficient conversion of carbohydrates into GVL. © 2019 Society of Chemical Industry
Development of a high‐efficiencytrans‐cinnamic acid bioproduction method by pH‐controlled separation technology
Authors: Zang Ying , Xia Meijuan , Zheng Zhaojuan , Ouyang Jia 
Published: April 2019

AbstractBACKGROUNDTrans‐cinnamic acid (t‐CA) and its derivatives have been shown to be antioxidant and antibacterial, and can be used in food additives and flavors. However, a traditionalt‐CA production via chemical synthesis with a condensation reaction may not be suitable for its application in the pharmaceutical and food fields as it is not a ‘green’ route. This study investigated the bioconversion ofl‐phenylalanine (l‐phe) tot‐CA using engineered whole‐cellEscherichia coliBL21(DE3) expressing phenylalanine ammonia‐lyase (PAL, EC 4.3.1.5) as a biocatalyst. To prevent product inhibition, anin situreaction and separation oft‐CA was performed using a pH‐controlled biotransformation strategy.RESULTSA coupling reaction and separation system based on pH adjustment was proposed for improving the bioproduction oft‐CA. Using this process, 39.61 g L−1t‐CA was bioproduced from 40 g L−1ofl‐phe in 8 h and a high purity (>98%) oft‐CA product was achieved directly without additional purification.CONCLUSIONThe bioprocess developed in this study is a highly efficient method for the bioproduction oft‐CA and, therefore, offers a promising alternative route fort‐CA production. © 2019 Society of Chemical Industry
Sickle cell carriers' unmet information needs: Beyond knowing trait status
Authors: Mayo‐Gamble Tilicia L. , Schlundt David , Cunningham‐Erves Jennifer , Murry Velma McBride , Bonnet Kemberlee , Quasie‐Woode Delores , Mouton Charles P. 
Published: April 2019

AbstractBenefits of identifying sickle cell disease (SCD) carriers include detection of at‐risk couples who may be informed on reproductive choices. Studies consistently report insufficient knowledge about the genetic inheritance pattern of SCD among people with sickle cell trait (SCT). This study explored perspectives of adults with SCT on the information needed to make an informed reproductive decision and the recommendations for communicating SCT information. Five focus groups (N = 25) were conducted with African Americans with SCT ages 18–65 years old. Participants were asked about their knowledge of SCT, methods for finding information on SCT, impact of SCT on daily living, and interactions with healthcare providers. An inductive‐deductive qualitative analysis was used to analyze the data for emerging themes. Four themes emerged, highlighting the unmet information needs of African American sickle cell carriers: (a) SCT and SCD Education; (b) information sources; (c) improved communication about SCT and SCD; and (d) increased screening strategies. Future studies are needed to determine effective strategies for communicating SCT information and to identify opportunities for education within community and medical settings. Identifying strategies to facilitate access to SCT resources and education could serve as a model for meeting unmet information needs for carriers of other genetic conditions.
Intestinal myiasis in a very elderly patient with inappropriate home care
Authors: Mizumoto Junki , Yamamoto Minako 
Published: April 2019

AbstractAn 83‐year‐old Japanese woman with dementia presented with multiple pressure ulcers and cellulitis. At presentation, numerous white maggots were wriggling in the stool on the diaper. Interview to the family revealed that the patient received inappropriate home care due to her dementia and her primary caregiver's developmental disability. No further maggots were omitted after admission, and she was diagnosed with intestinal myiasis. Intestinal myiasis has become rare in developed countries especially in adults. However, elderly bedridden patients living in poor hygiene conditions are at a greater risk of developing intestinal myiasis.
3D quantitative synthetic MRI‐derived cortical thickness and subcortical brain volumes: Scan–rescan repeatability and comparison with conventional T1‐weighted images
Authors: Fujita Shohei , Hagiwara Akifumi , Hori Masaaki , Warntjes Marcel , Kamagata Koji , Fukunaga Issei , Goto Masami , Takuya Haruyama , Takasu Kohei , Andica Christina , Maekawa Tomoko , Takemura Mariko Yoshida , Irie Ryusuke , Wada Akihiko , Suzuki Michimasa , Aoki Shigeki 
Published: April 2019

BackgroundPrevious quantitative synthetic MRI of the brain has been solely performed in 2D.PurposeTo evaluate the feasibility of the recently developed sequence 3D‐QALAS for brain cortical thickness and volumetric analysis.Study TypeReproducibility/repeatability study.SubjectsTwenty‐one healthy volunteers (35.6 ± 13.8 years).Field Strength/Sequence3D T1‐weighted fast spoiled gradient recalled echo (FSPGR) sequence was performed once, and 3D‐QALAS sequence was performed twice with a 3T scanner.AssessmentFreeSurfer and FIRST were used to measure cortical thickness and volume of subcortical structures, respectively. Agreement with FSPGR and scan–rescan repeatability were evaluated for 3D‐QALAS.Statistical TestsPercent relative difference and intraclass correlation coefficient (ICC) were used to assess reproducibility and scan–rescan repeatability of the 3D‐QALAS sequence‐derived measurements.ResultsPercent relative difference compared with FSPGR in cortical thickness of the whole cortex was 3.1%, and 89% of the regional areas showed less than 10% relative difference in cortical thickness. The mean ICC across all regions was 0.65, and 74% of the structures showed substantial to almost perfect agreement. For volumes of subcortical structures, the median percent relative differences were lower than 10% across all subcortical structures, except for the accumbens area, and all structures showed ICCs of substantial to almost perfect agreement. For the scan–rescan test, percent relative difference in cortical thickness of the whole cortex was 2.3%, and 97% of the regional areas showed less than 10% relative difference in cortical thickness. The mean ICC across all regions was 0.73, and 80% showed substantial to almost perfect agreement. For volumes of subcortical structures, relative differences were less than 10% across all subcortical structures except for the accumbens area, and all structures showed ICCs of substantial to almost perfect agreement.Data Conclusion3D‐QALAS could be reliably used for measuring cortical thickness and subcortical volumes in most brain regions.Level of Evidence: 3Technical Efficacy: Stage 1J. Magn. Reson. Imaging 2019;50:1834–1842.