bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lactococcus lactis subsp. cremoris C60 promotes immunoglobulin A production from B cells through functional modification of dendritic cells in intestinal environment
Authors: Saito Suguru , Okuno Alato , Kakizaki Nanae , Maekawa Toshio , Tsuji Noriko M 
Published: November 2024

Abstract

Probiotics utilizing lactic acid bacteria (LAB) have gained considerable attention in recent trends promoting self-managed health. Specifically, LAB-mediated immune modulation has been a key focus due to its potential to reduce the risk of pathogenic invasion and enhance host immunity. Immunoglobulin A (IgA) plays a crucial role in innate defense against various pathogens. Although only a limited number of probiotic LAB strains have been shown to increase IgA production, this capability remains of great interest. Here, we report a new strain, Lactococcus lactis subsp. cremoris C60 (C60), which promotes IgA production through functional modulation of intestinal B cells and dendritic cells (DCs). Heat-killed (HK)-C60 increased both pro-inflammatory and anti-inflammatory cytokine productions in DCs via the Toll-like receptor (TLR)- Myeloid differentiation primary response 88 (MyD88) signaling pathway, as demonstrated in both a physiological mouse model and in vitro cultures using bone marrow-derived dendritic cells (BMDCs). Notably, intragastric administration of HK-C60 significantly increased systemic IgA production, which was associated with the functional modification of B cells in the Peyer’s patches (PPs) of the small intestine. Immunophenotyping of PP cells from HK-C60-administered mice revealed both an expansion and functional upregulation of B cells. Mechanistically, we identified that DC-derived interleukin-6 (IL-6) and IL-10 play essential roles in the C60-mediated increase in IgA production by B cells. Finally, we evaluated the effect of C60 on IgA production using human peripheral blood mononuclear cells (PBMCs). Consistent with our findings in the mouse model, PBMCs produced IgA upon HK-C60 stimulation in an IL-6- and IL-10-dependent manner. Our results suggest that C60 is a novel probiotic strain capable of promoting intestinal immune homeostasis by upregulating IgA production, underscoring its potential for probiotic applications focused on immune conditioning.
Long-term oxygen treatment in chronic respiratory failure
Authors: Nuralieva G. S. , Shmidt A. E. , Avdeev I. S. , Nekludova G. V. 
Published: November 2024

Chronic respiratory failure (CRF) is the leading cause of mortality in patients with pulmonary diseases. One of the key treatments for CRF is long-term oxygen therapy (LTOT). The main purpose of LTOT is to improve the quality of life, increase physical performance, reduce the frequency of exacerbations and mortality of patients. It is recommended to use oxygen therapy for at least 15-16 hours a day, and if there is no effect, increase this time to 24 hours. Indications for LTOT have remained unchanged for a long time. The decision on the appointment of LTOT should be based on three-fold results of the gas composition of arterial blood, and the assessment of gas exchange parameters can be made only after stabilization of the condition (3-4 weeks after exacerbation). It is also worth considering the possibility of hypercapnia in patients and the likelihood of aggravation of the patient's condition due to oxygen-induced hypercapnia and respiratory acidosis. Despite the existence of numerous studies concerning the use of LTOT, most of them have been conducted on patients with COPD, which creates the need for a deeper study of the effectiveness of this method in patients with other diseases. The use of long-term oxygen therapy has a positive effect on the quality of life, physical performance, the frequency of exacerbations, hospitalizations and patient survival. There are various methods of oxygen delivery, which allows the use of LTOT not only at rest, but also when moving the patient over long distances. It is important to note that in addition to prescribing LTOT, patients need to change their lifestyle, stop smoking, and receive adequate drug therapy for the underlying disease.
Investigation of hydraulic parameters influencing interrill erosion under simulated rainfall conditions
Authors: Maaliou Aziz , Moussouni Abderzak , Ziani Dalila 
Published: November 2024

ABSTRACT

Interrill erosion is a major cause of land degradation. This study investigates the effects of slope and rainfall intensity on soil erosion and sediment loss. Two types of slopes were examined: interrill slope and a rill slope, artificially created in the center of a 1 m² soil plot. Experiments were conducted at the LEGHYD laboratory using a fixed-bed channel with a triangular cross-section to simulate the rill. Two slope values (3% and 5%) for the plot and two interrill slopes (15% and 22.5%) were tested. Sandy soil (70% sand, 30% gravel) was subjected to six artificial rainfall intensities: 101.94 mm·h−1, 90.39 mm·h−1, 81.89 mm·h−1, 69.49 mm·h−1, 37.82 mm·h−1, and 31.40 mm·h−1. Data on hydraulic parameters of overland flow were analyzed in relation to sediment concentration. Results showed that while slope had minimal impact on rill flow velocity, it significantly influenced rainfall intensity and overland flow power. A strong positive correlation was found between rainfall intensity, flow power, and sediment concentration. The findings emphasize the combined effects of slope and rainfall intensity on erosion, underscoring the importance of integrated soil management strategies for sustainable land use.
Evaluative criteria for least-cost economic analysis of citywide inclusive sanitation: A scoping review
Authors: Ross Simon , Fane Simon , Foster Tim 
Published: November 2024

ABSTRACT

Compared to health interventions, where evaluative criteria have been established, uncertainty about how comparative economic analysis methodologies should be applied to heterogeneous urban sanitation interventions in cities of low- and middle-income countries persists. Gaps between least-cost principles and current approaches constrain emerging citywide inclusive sanitation (CWIS) planning frameworks. Furthermore, a lack of comparable evidence about the economic efficiency of service provision interventions impacts the delivery of urban sanitation as an inclusive public service. This review scopes how least-cost principles may be applied to methodologies for urban sanitation interventions in two iterations. We devise and discuss evaluative criteria, embodying CWIS principles, that will improve the quality of these methodologies. They include how (i) the inclusion of diverse cost perspectives, particularly populations with marginal access to sanitation, are accommodated within an analysis; (ii) a context-specific without-project case may be used as an appropriate basis for comparison; (iii) tangible and intangible costs and outcomes may influence decisions; and (iv) diverse mixtures of interventions at different scales may be compared with integrity. The resulting evaluative criteria define principles that may inform future systematic reviews of methods aimed at enhancing CWIS planning frameworks.
The potential application of non-sewered sanitation systems in healthcare facilities – a case study in Beijing, China
Authors: Cheng Shikun , Zhao Qizhi , Wen Chongqiu , Manga Musa , Xiang Rui , Wang Xuemei , Li Zifu 
Published: November 2024

ABSTRACT

Non-sewered sanitation systems (NSSSs) could improve sanitation in healthcare facilities (HCFs) within urban areas of developing countries. However, they are not usually applied in these areas. In this study, the PEST analysis, which refers to analyzing the feasibility and potential of a project in terms of four factors – political, economic, social, and technological – was used to examine the practical application of NSSS in HCFs in Beijing, China. The results found that the support of NSSS applications in HCFs outweighs the challenges. Particularly in rural areas, NSSS has significant potential for application. Implementing these strategies would enhance sanitation in HCFs and reduce the spread of diseases.
Rice cultivation and safety concerns from irrigation with ZnO nanoparticle-contaminated treated municipal wastewater
Authors: Afriani Shinta Dhewi , Phung Luc Duc , Sasaki Yuka , Kumar Arun , Watanabe Toru 
Published: November 2024

ABSTRACT

The proliferation of metal-based nanoparticles in treated municipal wastewater (TWW) has raised environmental and health concerns. This study examined the health risks and effects of ZnO nanoparticles (NPs) in TWW on rice growth, yield, and nutrition. Continuous fertigation was used to irrigate rice plants with TWW containing 0, 2, and 5 mg L−1 of either ZnO NPs or ZnSO4. Results indicated that TWW with higher ZnO NP concentrations significantly reduced plant height and root mass but increased shoot biomass and rice yield. ZnO NPs or ZnSO4 exposure increased Zn accumulation in rice roots, shoots, and grains while reducing Cd levels by 30%. However, elevated Zn levels also increased toxic metal accumulation, such as As and Mo. The estimated daily intake values for As, Zn, and Mo increased by over 100, 29–47, and 14–18%, respectively, raising cumulative health risks from trace element exposure, with hazard index (HI) values exceeding the safe threshold (HI &gt; 1). Despite no acute toxicity in rice plants, the continuous use of ZnO NP-contaminated TWW presents significant long-term environmental and health risks. This highlights the urgent need for stringent monitoring and management of TWW quality to safeguard the sustainability of agricultural wastewater reuse.
Developing a universal equation to estimate the mass of dewatered wastewater sludge during biological digestion at mesophilic and thermophilic temperatures
Authors: Poorasgari Eskandar , Örmeci Banu 
Published: November 2024

ABSTRACT

A series of dewaterability tests were conducted on various types of sludges to establish a wholistic relationship between sludge water fractions. Sludge samples were obtained from batch and continuous sludge digesters, which were operated anaerobically and aerobically under mesophilic and thermophilic conditions. Dewaterability of the sludge samples and the distribution of water fractions were studied using centrifugation and thermal drying. Thickened waste activated sludge (T-WAS) contained 10-11 g bound water (BW)/g of total solids (TS), and it was more hydrophilic than primary and digested sludges. During anaerobic digestion, BW content fluctuated between 3.2 and 4.2 g BW/g TS. However, aerobic digestion at 55°C reduced the BW content of the mixed T-WAS + primary sludges from 3.7 to 2.1 g BW/g TS. A linear function was developed to correlate supernatant and BW mass fractions (R2 = 0.995). An equation was derived from the linear function to estimate the mass of dewatered sludge based on the TS concentration of the initial wet sludge. The developed expression is applicable to different kinds of wastewater sludges. Such an expression would be helpful for the designers and operators of sludge thickening and dewatering systems that use centrifugal separation.
Composition and concentrations of microplastics including tyre wear particles in stormwater retention pond sediments
Authors: Öborn Lisa , Österlund Heléne , Lorenz Claudia , Vianello Alvise , Lykkemark Jeanette , Vollertsen Jes , Viklander Maria 
Published: November 2024

ABSTRACT

Stormwater is recognised as a vector for microplastics (MPs), including tyre wear particles (TWPs) from land-based sources to receiving waterbodies. Before reaching the waterbodies, the stormwater may be treated. In this study, sediments from six treatment facilities (five retention ponds and a subsurface sedimentation tank) were analysed to understand MP occurrence, concentrations, sizes, polymer types and distribution between inlet and outlet. The concentrations of MPs showed large variations between and within different facilities with MP concentrations of 1,440–72,209 items/kg (analysed by μFTIR) corresponding to 120–2,950 μg/kg and TWP concentrations from &lt;DL up to 69,300 μg/kg (analysed by pyrolysis–GC–MS), with significantly higher concentrations at the inlet compared to the outlet. Polypropylene (PP) was the predominant MP type in terms of number in all samples. TWPs were dominant by mass in most (nine) samples. The relatively low density of PP polymers implies that density might not be the sole factor influencing particle settlement behaviour. Small particles occurred more frequently than large ones; around 70% of the particles detected in the samples were 100 μm or smaller. In summary, this study highlights the occurrence of MPs, including TWPs, in stormwater facilities and demonstrates variations in concentrations depending on sites and locations within the facility.
Pressure Ulcers and Nutrients: From Established Evidence to Gaps in Knowledge
Authors: Kassym Laura , Zhetmekova Zhuldyz , Kussainova Assiya , Semenova Yuliya , Vetrova Anastassiya , Nurzhan Sholpan , Sarbassova Gauhar , Akhmetova Almira , Orazalina Ainash , Uzbekova Saltanat , Bjørklund Geir 
Published: November 2024

Abstract:
Pressure ulcers (PUs) are caused by continuous pressure or friction on the
skin that damages tissue, especially over bony prominences. A critical factor in the development
and progression of PUs is poor nutritional status, which often involves deficiencies
in essential nutrients such as proteins, vitamins (A, C, D, E, K, and the B complex),
and trace elements (including zinc, selenium, copper, iron, and manganese). These
micronutrients are vital for effective wound healing, as they play significant roles in
cellular repair, immune function, and tissue regeneration. Laboratory tests for serum albumin,
prealbumin, transferrin, retinol-binding protein, and anthropometric measures
like height, weight, and body mass index (BMI) are used to evaluate a patient's nutritional
status. Screening tools such as the Mini Nutritional Assessment (MNA), Malnutrition
Universal Screening Tool (MUST), LPZ questionnaire, and Subjective Global Assessment
(SGA) are commonly employed. Emerging evidence from various studies, including
in vitro, in vivo, and clinical trials, underscores the importance of personalized nutritional
interventions in managing PUs. Unlike generic dietary plans, tailored nutrition
that addresses the specific needs of individuals shows greater potential in promoting
wound healing and improving clinical outcomes. This synthesis of existing research
highlights the critical influence of micronutrients on the healing process of PUs. It
suggests that a personalized approach to nutrition, which takes into account individual
patient requirements and deficiencies, is likely to be more effective than a one-size-fits-
all strategy in the management of these complex wounds.
Advances in Discovery and Design of Anti-influenza Virus Peptides
Authors: Li Shixin , Xie Xi , Zhou Shaofen , He Jian 
Published: November 2024

Abstract:
The influenza virus, a well-known pathogen that causes respiratory illness, remains
an important global health threat because of the significant morbidity and mortality
rates of people infected with the virus annually. The influenza virus undergoes frequent
antigenic variation, and with the increasing frequency of resistant influenza strains
against existing antiviral drugs, there is an urgent need for the development of new anti-
influenza treatment strategies. Peptides have the potential to offer high potency, selectivity,
and relatively low drug resistance. As such, the design and screening of novel anti-
influenza virus peptides with high potency have become increasingly important in an
effort to fight global influenza epidemics. Herein, we introduce three approaches to developing
anti-influenza virus peptides: discovery from natural products, library construction
for antiviral peptide screening, and rational design based on functional regions of influenza
viral proteins. This review summarizes recent progress in the discovery and design
of anti-influenza virus peptides over the past 20 years.
KIAA1429 Promotes Keloid Formation Through the TGF-Β1/Smad Pathway
Authors: Ren Shuai , Ji Yingchang , Wang Mengmeng , Ye Maodong , Huang Lvdong , Cai Xiangna 
Published: November 2024

Background:
Keloid formation is characterized by excessive production of
extracellular matrix, leading to dysregulated fibroproliferative collagen response. N6-
methyl-adenosine (m6A) modification plays an essential role in this process.

Objective:
Our objective in this study was to explore the mechanism of m6A
methyltransferase KIAA1429 in keloid formation.

Methods:
We examined the impact of m6A methyltransferase KIAA1429 on keloid
formation using qRT-PCR, Western blot, immunofluorescence, Transwell migration
assay, and MeRIP-qPCR.

Results:
KIAA1429 was downregulated in keloid tissue. Overexpression of KIAA1429
suppressed fibroblast migration and reduced COL1A1 and α-SMA levels. Conversely,
the knockdown of KIAA1429 promoted fibroblast migration and COL1A1 and α-SMA
levels. Additionally, overexpression of KIAA1429 inhibited the TGF-β1/Smad pathway.
Mechanistic experiments suggested that KIAA1429 regulated TGF-β1 m6A
modification, maintained TGF-β1 mRNA stability, and participated in the regulation of
keloid formation. Furthermore, TGF-β1 could reverse the effects of KIAA1429
overexpression on fibroblast migration and collagen deposition.

Conclusion:
Taken together, our study suggested that KIAA1429 promoted keloid
formation through the TGF-β1/Smad pathway, providing new insights for the treatment
of keloid.
Upadacitinib and Cardiovascular Adverse Events in Rheumatoid Arthritis: A Systematic Review and Meta-Analysis
Authors: Omidi Fatemeh , Delkash Parisa , Nasiri Mohammad Javad , Mirsaeidi Mehdi 
Published: November 2024

Background:
Upadacitinib, a Janus kinase (JAK) inhibitor used in rheumatoid arthritis
treatment, has prompted safety concerns due to potential cardiovascular adverse events. However,
current evidence does not provide a definitive conclusion.

Methods:
We conducted a comprehensive systematic review of the literature up until March 15,
2024, utilizing databases like PubMed/Medline, Embase, and Cochrane CENTRAL. A meta-analysis
approach was used to derive pooled odds ratios (OR) along with their 95% confidence intervals
(CI) to assess the cardiovascular risk associated with upadacitinib. Publication bias was evaluated
using Begg's and Egger's tests.

Results:
Our meta-analysis included six studies with a total of 4,202 participants. For the 15 mg
dosage of Upadacitinib, the pooled OR was 1.20 (95% CI: 0.3-4.3), indicating a nominal, non-significant
increase in the risk of cardiovascular adverse events. Analysis of the 30 mg dosage presented
a pooled OR of 2.37 (95% CI: 0.6-9.1), pointing to a higher, yet statistically insignificant,
potential risk. The absence of publication bias was confirmed through Begg's and Egger’s tests.

Conclusion:
The analysis suggests a potential heightened cardiovascular risk associated with Upadacitinib,
more so with the 30 mg dosage. Nevertheless, the lack of statistical significance and the
wide confidence intervals necessitate a prudent approach to these findings. Tailored treatment
strategies, rigorous monitoring, and further empirical studies are crucial for refining the safety profile
of upadacitinib and ensuring optimal patient outcomes.
Advancements in Colloidosome Fabrication, Functionalization, and Applications: A Comprehensive Review
Authors: Komal  , Singh Lovekesh 
Published: November 2024

Abstract:
Among the recent developments in the field of microcapsules, colloidosomes microcapsules
made of colloidal particles have elicited much interest in promising perspectives in microencapsulation
technologies. First reported by Dinsmore et al., these structures hold a lot of potential
in different areas of application, which include gene delivery, targeting the brain, and tumor treatment.
However, the challenges associated with their permeability and stability limit the use of these
coatings. Nevertheless, these problems define colloidosomes as possessing some features of
protocells that include metabolic activities and response to stimuli, which make them significant
in synthetic biology and biomimetic systems. In drug delivery, colloidosomes have the potential
of providing solubility, stability, and a controlled rate of drug release, which will have an impact
on the therapeutic use of drugs. Fabrication of colloidosomes is done in many ways, and the common
ones include the mulsion strategy, layer adsorption, and microfluidics strategy. Some of these
fabrication techniques have been investigated in the recent past to determine how their application
can lead to enhanced colloidosome characteristics. Some of these methods include spray drying,
sol-gel processes, and electrostatic assembly. For example, the study by Payizila Zulipiker et
al. showed that different levels of permeability are controllable through the use of spray drying
technology. Another study by Jia Jia et al. presented the preparation of pH-responsive colloidosomes
and the high efficiency of encapsulation. Consequently, improvements in the manufacturing
process and new self-assembly technologies that include microfluidics systems have also improved
the creation of colloidosomes. New advancements in the field of colloidosome synthesis,
as well as their surface modification, will open up numerous application prospects in the fields of
pharmaceutics, regenerative medicine, and bioinspired materials science. New trends range from
3D printing to stimuli-responsive materials and design, as well as hybrid systems that are application-
specific. Despite the difficulties that remain to be faced, colloidosomes represent an extraordinary
opportunity for the growth of biotechnology and materials science to open the path to new
generations of drugs and bioinspired applications.
Comparative Dissolution Study of Ciprofloxacin Hydrochloride-sustained Release Tablets by Using Carbopol-934 and Guar Gum Polymers
Authors: Mollick Mirazuddin , Ashique Sumel , Bhowmick Mithun , Rahaman Syed Mustafizur , Bhowmick Pratibha , Katari Shreyasi 
Published: November 2024

Aims:
This study aimed to identify the potential release of the drug from the tablet dosage
form using Carbopol-934 and Guar Gum polymers at different concentration ratios.

Objective:
The objective of this study was to determine the cumulative percentage of ciprofloxacin
hydrochloride released from different polymers.

Method:
We formulated ciprofloxacin hydrochloride sustained-release tablets with Carbopol-934
and Guar gum using the wet granulation method. For the formulation of sustained-release ciprofloxacin
hydrochloride tablets, both polymers were mixed in varying ratios. The tablets were examined
for pre-formulation studies, including the angle of repose, bulk density, compressibility index, and
physiological properties, such as variations in weight, friability, and drug content. For postformulation
studies, such as the in vitro study, the in vitro release of the drugs was evaluated in a
phosphate buffer solution (pH 7.4) for 8 h. However, the physiological properties of ciprofloxacin
hydrochloride tablets were limited due to their liberality.

Results:
The tablets containing guar gum (Batches B-I and B-II) showed better drug content than
other polymer-based batches, such as Carbopol-934 (Batch A-I, A-II) and a mixture of both polymer
Carbopol-934 and Guar gum (Batch C-I, C-II). Sustained drug release was observed in the guar gum
polymer-based tablets (Batch B-II). It was observed that the dissolution profile of ciprofloxacin hydrochloride
tablets formed using different polymers showed an increase in the polymer ratio, which
can be related to the increase in drug release.

Conclusion:
From this study, we can conclude that the ciprofloxacin hydrochloride drug release
percentage was maximum when guar gum polymer was used in the tablet dosage form.