bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comment on Lykova et al. (2024): “Natromelansonite, Na3Zr[Si7AlO19]⋅4–5H2O, a new member of the rhodesite mero-plesiotype series from Mont Saint-Hilaire, Quebec, Canada”
Authors: Gore Thomas E. , McDonald Andrew M. 
Published: October 2024

AbstractIn this communication, we present a brief response to Lykova et al. (2024) who, in a paper in volume 88 https://doi.org/10.1180/mgm.2024.1 (this journal), describe the occurrence of the new mineral natromelansonite, Na3Zr(Si7AlO19)⋅4–5H2O, and provide commentary on the related species, melansonite (Na,□)□2KZrSi8O19⋅5H2O. The comments in question revolve primarily around the roles and abundances of Al and Na in both minerals, and how their incorporation is best represented in their idealised formulae.Lykova et al. (2024) state that Al is an essential component of both melansonite and natromelansonite and should thus be reflected in the ideal formulae. With respect to Na, they propose that the ideal formula of melansonite contains two Na apfu rather than one, as was presented in our initial definition of the mineral.We reiterate there is no unequivocal evidence that Al is an essential component of melansonite, occupying a unique crystallographic site, but rather, it only substitutes for Si. Secondly, though the true Na content of melansonite is probably higher than were originally reported (this being simply explained by the thin nature of the crystals analysed and the volatility of Na under the electron beam), it cannot reconcile the doubling of the Na apfu for melansonite, as was suggested by Lykova et al. (2024).
Forimtamig, a novel GPRC5D-targeting T-cell bispecific antibody with a 2+1 format, for the treatment of multiple myeloma
Authors: Eckmann Jan , Fauti Tanja , Biehl Marlene , Zabaleta Aintzane , Blanco Laura , Lelios Iva , Gottwald Stefan , Rae Richard , Lechner Stefanie , Bayer Christa , Dekempe Quincy , Osl Franz , Carrié Nadege , Kassem Sahar , Lorenz Stefan , Christopeit Tony , Carpy Alejandro , Bujotzek Alexander , Bröske Ann-Marie , Dekhtiarenko Iryna , Attig Jan , Kunz Leo , Cremasco Floriana , Adelfio Roberto , Fertig Georg , Dengl Stefan , Gassner Christian , Bormann Felix , Kirstenpfad Claudia , Kraft Thomas , Diggelmann Sarah , Knobloch Melanie , Hage Carina , Feddersen Romi , Heidkamp Gordon , Pöschinger Thomas , Mayoux Maud , Bernasconi Luise , Prosper Felipe , Dumontet Charles , Martinet Ludovic , Leclair Stéphane , Xu Wei , Paiva Bruno , Klein Christian , Umaña Pablo 
Published: October 2024

Abstract
Despite several approved therapies, multiple myeloma (MM) remains an incurable disease with high unmet medical need. “Off-the-shelf” T-cell bispecific antibodies (TCBs) targeting B-cell maturation antigen (BCMA) and G protein–coupled receptor class C group 5 member D (GPRC5D) have demonstrated high objective response rates in heavily pretreated patients with MM; however, primary resistance, short duration of response, and relapse driven by antigen shift frequently occur. Although GPRC5D represents the most selective target in MM, recent findings indicate antigen loss occurs more frequently than with BCMA. Thus, anti-GPRC5D immunotherapies must hit hard during a short period of time. Here, we characterize forimtamig, a novel GPRC5D-targeting TCB with 2+1 format. Bivalent binding of forimtamig to GPRC5D confers higher affinity than classical 1+1 TCB formats correlating with formation of more stable immunological synapses and higher potency in tumor cell killing and T-cell activation. Using an orthotopic mouse model of MM, forimtamig recruited T effector cells to the bone marrow and induced rapid tumor killing even after the introduction of step-up dosing to mitigate cytokine release. Combination of forimtamig with standard-of-care agents including anti-CD38 antibodies, immunomodulatory drugs, and proteasome inhibitors improved depth and duration of response. The combination of forimtamig with novel therapeutic agents including BCMA TCB and cereblon E3 ligase modulatory drugs was potent and prevented occurrence of GPRC5D -negative tumor relapse. Forimtamig is currently being evaluated in phase 1 clinical trials in patients with relapsed and refractory MM for monotherapy and in combination treatments. This trial was registered at www.ClinicalTrials.gov as #NCT04557150.
Endometriosis development in relation to hypoxia: a murine model study
Authors: Hoffmann-Młodzianowska Marta , Maksym Radosław B. , Pucia Katarzyna , Kuciak Monika , Mackiewicz Andrzej , Kieda Claudine 
Published: October 2024

Abstract
Background
Endometriosis, due to its ambiguous symptoms, still remains one of the most difficult female diseases to treat, with an average diagnosis time of 7–9 years. The changing level of hypoxia plays an important role in a healthy endometrium during menstruation and an elevated expression of the hypoxia-inducible factor 1-alpha (HIF-1α) has been demonstrated in ectopic endometria. HIF-1α mediates the induction of proangiogenic factors and the development of angiogenesis is a critical step in the establishment and pathogenesis of endometriosis. Although the inhibition of angiogenesis has been proposed as one of the actionable therapeutic modalities, vascular normalization and re-oxygenation may become a possible new approach for therapeutic intervention.

Methods
Our goal was to investigate whether a selected murine model of endometriosis would be suitable for future studies on new methods for treating endometriosis. Non-invasive, high-resolution ultrasound-monitored observation was selected as the preclinical approach to obtain imaging of the presence and volume of the endometriotic-like lesions. The EF5 (2-(2-Nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide) compound that selectively binds to reduced proteins in hypoxic cells was used for hypoxia detection. The expression of Pten and other crucial genes linking endometriosis and hypoxia were also assessed.

Results
Using EF5, a pentafluorinated derivative of the 2-nitroimidazole that is metabolically reduced by oxygen-inhibitable nitroreductase, we confirmed that hypoxia did develop in the selected model and was detected in uterine and ectopic endometriotic lesions. Moreover, the changes in oxygen tension also influenced the expression level of significant genes related to endometriosis, like Pten, Trp53, Hif1a, Epas1, and Vegfa. Their strong modulation evidenced here is indicative of model reliability. Using high-resolution ultrasound-based imaging, we present a non-invasive method of visualization that enables the detection and observation of lesion evolution throughout the duration of the experiment, which is fundamental for further preclinical studies and treatment evaluation.

Conclusions
The selected model and method of visualization appear to be suitable for the study of new treatment strategies based on hypoxia alleviation and blood flow restoration.
Chemokine CXCL13–CXCR5 signaling in neuroinflammation and pathogenesis of chronic pain and neurological diseases
Authors: Zheng Kaige , Chen Muyan , Xu Xingjianyuan , Li Peiyi , Yin Chengyu , Wang Jie , Liu Boyi 
Published: October 2024

AbstractChronic pain dramatically affects life qualities of the sufferers. It has posed a heavy burden to both patients and the health care system. However, the current treatments for chronic pain are usually insufficient and cause many unwanted side effects. Chemokine C–X–C motif ligand 13 (CXCL13), formerly recognized as a B cell chemokine, binds with the cognate receptor CXCR5, a G-protein-coupled receptor (GPCR), to participate in immune cell recruitments and immune modulations. Recent studies further demonstrated that CXCL13–CXCR5 signaling is implicated in chronic pain via promoting neuroimmune interaction and neuroinflammation in the sensory system. In addition, some latest work also pointed out the involvement of CXCL13–CXCR5 in the pathogenesis of certain neurological diseases, including ischemic stroke and amyotrophic lateral sclerosis. Therefore, we aim to outline the recent findings in regard to the involvement of CXCL13–CXCR5 signaling in chronic pain as well as certain neurological diseases, with the focus on how this chemokine signaling contributes to the pathogenesis of these neurological diseases via regulating neuroimmune interaction and neuroinflammation. Strategies that can specifically target CXCL13–CXCR5 signaling in distinct locations may provide new therapeutic options for these neurological diseases.
Joint effects of depression and social determinants of health on mortality risk among U.S. adults: a cohort study
Authors: Wang Zun , Pu Boxuan 
Published: October 2024

Abstract
Background
Unfavorable social determinants of health (SDoH) are associated with depression. Both depression and SDoH are associated with increased risks of mortality, but their joint impacts on mortality risks remain unclear. This study aims to investigate the joint effects of depression and SDoH on mortality risk.

Methods
Utilizing data from the National Health and Nutrition Examination Survey (NHANES) 2007–2018, 24,727 adults aged ≥ 20 were included. SDoH was assessed based on the 5 domains outlined in the U.S. Healthy People 2030 initiative. The cumulative number of unfavorable SDoH was calculated and categorized into low and high burden levels. The definition of depression was based on the Patient Health Questionnaire-9 (PHQ-9) scores ≥ 10. The joint associations of depression and SDoH with all-cause, cardiovascular disease (CVD), and cancer mortality were examined using Cox proportional hazard models.

Results
We identified 2,377 (6.84%) all-cause deaths (CVD, 717; cancer, 606) during a median follow-up of 7.0 years. Depression was associated with increased mortality risks, and SDoH could explain 32.4% and 28.3% of the associations between depression and all-cause and CVD mortality, respectively. No significant interactions were observed between depression and SDoH on mortality. However, a low burden of unfavorable SDoH reduced the risk of all-cause mortality in depressed patients (hazard ratio [HR], 0.58; 95% confidence interval [CI]: 0.36–0.92). In the joint analysis, individuals with both depression and a high burden of unfavorable SDoH had the highest risks of all-cause and CVD mortality. Specifically, compared with individuals with no depression and a low burden of unfavorable SDoH, those with depression and a high burden of unfavorable SDoH had higher risks of all-cause (HR, 2.52; 95% CI: 2.01–3.18) and CVD mortality (HR, 2.79; 95% CI: 1.95–3.99).

Conclusion
Adults with both depression and a high burden of unfavorable SDoH had the highest risks of all-cause mortality and CVD mortality. The result suggests considering depression and SDoH jointly in developing targeted intervention strategies to improve survival outcomes and calls for larger cohort studies and clinical trials to validate our findings.
Enhancing core components for a digital “healthy eating” resource in early childhood and care settings: staff’s perceptions and needs
Authors: Helland Sissel H. , Bjørkkjaer Tormod , Vejrup Kristine , Øverby Nina C. 
Published: October 2024

Abstract
Background
In response to the growing need for effective programmes to promote healthy eating habits among children in real-world settings, we conducted a qualitative study. Our aim was to explore the content-related needs of early childhood education and care (ECEC) staff regarding the core components of an upcoming digital “healthy eating” resource. This resource, designed for real-world implementation through the Nutrition Now project, seeks to enhance children’s nutrition and health within ECEC settings.

Methods
Twelve individual semi-structured interviews with ECEC staff in a Norwegian municipality were conducted. Subsequently, we conducted two focus group discussions, involving five participants, to encourage reflection on the preliminary findings from the individual interviews. Qualitative thematic analysis was conducted using Nvivo software. Data from the interviews and focus groups were transcribed verbatim and analysed thematically to identify and summarise staff’s subjective experiences and reasoning.

Results
Six themes were identified for the development of the upcoming digital “healthy eating” resource: (1) A comprehensive recipe bank, and menu suggestions, (2) Tips for easy and quick cooking, simple hygiene and allergy management, (3) Age-appropriate food learning ideas, (4) Strategies for mealtime learning and engagement, (5) Alignment with the national ECEC curriculum, and (6) Parent-friendly access and content.

Conclusions
This research provides valuable guidance and informs the adaptation of an expert-led digital “healthy eating” intervention to better suit ECEC staff and the ECEC context, consequently enhancing its feasibility and effectiveness.

Trial registration
This study was not registered in a trial registry as it is not a clinical trial or intervention study but serves as a pilot for the Nutrition Now study, trial identifier ISRCTN10694967 (https//doi.org/10.1186/ISRCTN10694967), registration date 19/06/2022.
Fusarium verticillioides pigment: production, response surface optimization, gamma irradiation and encapsulation studies
Authors: Mwaheb Mai Ali , Hasanien Yasmeen A. , Zaki Amira G. , Abdel-Razek Alaa S. , Al Halim Laila R. Abd 
Published: October 2024

Abstract
Background
Natural pigments are becoming more significant because of the rising cost of raw materials, pollution, and the complexity of synthetic pigments. Compared to synthetic pigments, natural pigments exhibit antimicrobial properties and is less allergic. Pigments from microbial sources could easily be obtained in an inexpensive culture media, produced in high yields, and microbes are capable of producing different colored pigments. Searching for new sources for natural pigments to replace synthetic ones in food applications has become an urgent necessity, but the instability of these compounds is sometimes considered one of the obstacles that reduce their application. Encapsulation provides an ideal solution for natural dye protection through a controlled release strategy. Thus, this study aims at isolation of several soil fungi and subsequent screening their pigment production ability. The chosen pigment-producing fungal strain underwent full identification. The produced pigment was extracted with ethyl acetate and estimated spectrophotometrically. As there is a necessity to obtain a high pigment yield for efficient industrial application, the best production medium was tested, optimum conditions for maximum dye production were also investigated through the response surface methodology, and gamma irradiation was also employed to enhance the fungal productivity. Encapsulation of the produced pigment into chitosan microsphere was tested. The pigment release under different pH conditions was also investigated.

Results
A new strain, Fusarium verticillioides AUMC 15934 was chosen and identified for a violet pigment production process. Out of four different media studied, the tested strain grew well on potato dextrose broth medium. Optimum conditions are initial medium pH 8, 25 °C-incubation temperature, and for 15-day incubation period under shaking state. Moreover, a 400 Gy irradiation dose enhanced the pigment production. Chitosan microsphere loaded by the pigment was successfully prepared and characterized by infrared spectroscopy and scanning electron microscopy.

Conclusion
This irradiated Fusarium strain provides a more economically favorable source for production of a natural violet dye with an optimum productivity, enhanced yield, and improved properties (such as, enhanced stability, controlled release, and bioaccessibility) by encapsulation with chitosan for efficient application in food industry.
Effect of soft tissue thickness on accuracy of conventional and digital implant impression techniques
Authors: Awad Eman Mostafa , ElSheikh Mohamed Maamoun , El-Segai Azza Abd el moneim 
Published: October 2024

Abstract
Background
Placing implants deep sub-gingivally may affect the accuracy of implant impression techniques and the fit of final restoration.

Purpose
The aim of this in-vitro study was to evaluate the effect of soft tissue thickness on accuracy of conventional and digital implant impression techniques.

Methods
Four parallel implant analogues (A, B, C, D) placed in each of two epoxy resin models representing edentulous mandible covered by flexible polyurethane material with two different thickness two mm and four mm. A total of sixty impressions performed, thirty impressions for each model divided into four groups (n = 15 per group) GI (C2mm) open tray impression with two mm implant depth, GII (C4mm) open tray impression with four mm implant depth, GIII (D2mm) digital impression with two mm implant depth, GIV (D 4 mm) digital impression with four mm implant depth. Impressions from open tray technique were poured to get stone casts while impressions from digital scanning technique were printed as three-dimensional printed casts. The six inter-implant distances between analogues were measured using Co-ordinate measuring machine, deviations compared to reference models were calculated. Data was collected, tabulated and statistically analyzed using One-way ANOVA test to detect significances between groups.

Results
For conventional impressions there was significant difference between C2mm/C4mm (P < 0.001) regarding interimplant distance, while in digital impressions there was no significant difference between D2mm/D4mm AB(p = 0.110), BC(p = 0.066), CD(p = 0.710), AD(p = 0.084), AC(p = 0.067) and BD(p = 0.072). There was significant difference between conventional and digital impression techniques C2mm/D2mm, C4mm/D4mm (P < 0.001).

Conclusion
Within the limitations of this in-vitro study digital impressions provide more accurate outcomes with implants placed deeper subgingivally than conventional impressions.

Trial registration
Retrospectively registered.
Enhancement of early proximal caries annotations in radiographs: introducing the Diagnostic Insights for Radiographic Early-caries with micro-CT (ACTA-DIRECT) dataset
Authors: Valenzuela Ricardo E. Gonzalez , Mettes Pascal , Loos Bruno G. , Marquering Henk , Berkhout Erwin 
Published: October 2024

Abstract
Background
Proximal caries datasets for training artificial intelligence (AI) algorithms commonly include clinician-annotated radiographs. These conventional annotations are susceptible to observer variability, and early caries may be missed. Micro-computed tomography (CT), while not feasible in clinical applications, offers a more accurate imaging modality to support the creation of a reference-standard dataset for caries annotations. Herein, we present the Academic Center for Dentistry Amsterdam—Diagnostic Insights for Radiographic Early-caries with micro-CT (ACTA-DIRECT) dataset, which is the first dataset pairing dental radiographs and micro-CT scans to enable higher-quality annotations.

Methods
The ACTA-DIRECT dataset encompasses 179 paired micro-CT scans and radiographs of early proximal carious teeth, along with three types of annotations: conventional annotations on radiographs, micro-CT-assisted annotations on radiographs, and micro-CT annotations (reference standard). Three dentists independently annotated proximal caries on radiographs, both with and without micro-CT assistance, enabling determinations of interobserver agreement and diagnostic accuracy. To establish a reference standard, one dental radiologist annotated all caries on the related micro-CT scans.

Results
Micro-CT support improved interobserver agreement (Cohen’s Kappa), averaging 0.64 (95% confidence interval [CI]: 0.59–0.68) versus 0.46 (95% CI: 0.44–0.48) in its absence. Likewise, average sensitivity and specificity increased from 42% (95% CI: 34–51%) to 63% (95% CI: 54–71%) and from 92% (95% CI: 88–95%) to 95% (95% CI: 92–97%), respectively.

Conclusion
The ACTA-DIRECT dataset offers high-quality images and annotations to support AI-based early caries diagnostics for training and validation. This study underscores the benefits of incorporating micro-CT scans in lesion assessments, providing enhanced precision and reliability.

Graphical Abstract
Collaborative work processes in establishing a MiniMaria treatment center for youth substance addiction: a qualitative inquiry of county council healthcare and municipal efforts
Authors: Lindberg Maria , Kihlström Jofen , Hylander Irene , Salzmann-Erikson Martin 
Published: October 2024

Abstract
Background
This article is part of a larger study exploring the collaborative dynamics between key stakeholders in providing care to youths suffering from alcohol or substance use and their families in formulating policies and operational practices for county and country-wide application in similar settings. The focus of this article is to describe the collaborative processes between two stakeholders, a municipality, and a county council, in establishing a MiniMaria treatment center. While collaborative efforts between municipalities and county councils in health service provision are often acknowledged, little is known about how communication and decision-making processes between these entities shape the success of such initiatives. This study aims to fill this gap by providing insights into the communicative processes that foster organizational cohesion, agility, and innovation. The guiding research question is: What communicative processes occur between the county council and municipal stakeholders during the planning phase of the MiniMaria treatment center?

Methods
The municipality and county council were selected based on purposive sampling, owing to the proximity and accessibility of the field. An exploratory and descriptive design, incorporating a participatory research approach, was employed for this qualitative investigation.

Results
Two central themes, each underpinned by specific subthemes sum up the essence of our findings. The first theme underscores the collaborative dynamics and shared objectives that have emerged through the project, thus showing the importance of a unified vision and mutual understanding in driving the initiative forward. The second theme points to the practical aspects of implementing the project, including recruitment strategies, and the significance of interpersonal communication.

Conclusions
This article sheds light on the establishment of a MiniMaria treatment center through collaboration between a municipality and county council, using the Four Flows Model to interpret communicative processes. Membership negotiation was crucial for defining roles and building a unified team identity, while activity coordination ensured aligned stakeholder efforts. Self-structuring facilitated internal organization and operational clarity, and institutional positioning aligned the initiative with broader healthcare norms, enhancing its credibility and impact. These communicative practices were central to get a grip on inter-organizational complexities, emphasizing communication’s constitutive role in organizational development and innovation.