bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGEMBANGAN CABAI KATOKKON (Capsicum annuum L. var. sinensis) DI LUAR HABITAT ASLINYA
Authors: Ibrahim Bakhtiar , Numba Sudirman , Hazisah Nur 
Published: October 2024

This research aims to develop Katokkon chilies outside their natural habitat with different environmental conditions. Increase soil fertility levels in areas with low fertility levels by adding manure and NPK fertilizer. The study used a two-factor randomized block design (RBD): NPK fertilizer and cow manure. NPK fertilizer consisted of 2 grams, 4 grams, 6 grams per 10 kg of soil, and control (without NPK fertilizer). Cow manure treatment consisted of 0.5 kg, 1 kg, and 1.5 kg per 10 kg of soil and without cow manure (control). Each treatment was repeated 3 times and consisted of 3 plants for each observation unit. The research results show that developing katokkon chili plants outside their natural habitat is still possible. Providing 0.5 kg of cow manure had the best effect on the parameters of plant height, fruit weight, and production of Katokkon chili plants. Adding 2 g NPK fertilizer provided the best growth in terms of plant height, fruit weight, and production parameters. However, the addition of 6 g NPK fertilizer affected the productive branch parameters of Katokkon chili plants. There was a real interaction between 0.5 kg cow manure and the addition of 2 g NPK fertilizer on the parameters of plant height, fruit weight per plant, and production of Katokkon chili plants
EFISIENSI PEMANFAATAN PUPUK ANORGANIK MELALUI APLIKASI BOKASHI GAMAL PADA TANAMAN CABAI MERAH BESAR HIBRIDA (Capsicum annuum L.)
Authors: Alimuddin Suraedah , Edy Edy , Haris Abdul 
Published: October 2024

The purpose of this study was to determine the efficiency of inorganic fertilizer utilization through the use of bokashi from Gliricidia sepium on large hybrid chili plants and its effects on the physical and chemical properties of the soil as well as plant growth and productivity. This study was conducted in Pakkabba Village, North Galesong District, Takalar Regency, and soil, bokashi, and leaf tissue analysis was carried out at the Soil Laboratory of the Agricultural Assessment and Technology Center (BPTP) Maros. This research was conducted using a Randomized Block Design with 8 treatments: 100% inorganic fertilizer (ZA 650, Urea 250, SP36 500, and KCl 450 kg.ha-1); 90% inorganic fertilizer + Bokashi of Gliricidia sepium 5 t.ha-1; 80% inorganic fertilizer + Bokashi of Gliricidia sepium 10 t.ha-1; 70% inorganic fertilizer + Bokashi of Gliricidia sepium 15 t.ha-1; 60% inorganic fertilizer + Bokashi of Gliricidia sepium 20 t.ha-1; 50% inorganic fertilizer + Bokashi of Gliricidia sepium 25 t.ha-1; Without inorganic fertilizer + Bokashi Gliricidia sepium 30 t.ha-1. The results showed that the application of Gliricidia sepium bokashi did not have a significant effect on the level of soil porosity. The application of Gliricidia sepium bokashi increased the residue of N, P, K soil, C-organic, CEC, and soil pH at the end of the study. Inorganic fertilizer 70% of the recommended dose + 15 t.ha-1 Gliricidia sepium bokashi provided significantly better hybrid large chili productivity compared to other treatments, namely 22.11 t.ha-1. Gliricidia sepium bokashi 30 t.ha-1 without inorganic fertilizer produced chili productivity that was no different from the treatment of 100% inorganic fertilizer. These results indicate that the use of 30 t.ha-1 Gliricidia sepium bokashi can save 100% of inorganic fertilizer (650 kg ZA, 250 kg urea, 500 kg SP-36, and 450 kg KCl) in large chili plantings so that the use of 30 t.ha-1 Gliricidia sepium bokashi without inorganic fertilizer can be an alternative for organic cultivation of hybrid large chili plants.
Lesion Characterization by 99mTc-MIBI Myocardial Perfusion Imaging (MPI) & 18F-FDG Cardiac PET Following Myocardial Infarction Before Revascularization & Outcome Prediction: Initial Experience in Bangladesh
Authors: Akhter Afroza , Biswas Shankar Kumar , Sharmin Shaila , Islam Sabrina , Begum Rubina , Haque Jasmine Ara , Biswas Pijous , Chowdhury Abdul Wadud 
Published: October 2024

99mTc-MIBI SPECT myocardial perfusion imaging (MPI) and 18F-FDG cardiac positron emission tomography (Cardiac PET) can assess the extent and severity of myocardial perfusion and metabolic defect following myocardial infarction (MI) and also able to predict the outcome before revascularization in known coronary artery disease patients. About 135 cases of known coronary artery disease patients were enrolled here for combined nuclear imaging tests, rest only ECG gated MPI and FDG cardiac PET to assess myocardial perfusion and viability status following MI prior to revascularization. In this study we want to figure out the utility of this combined protocol for myocardial lesion characterization and its role for clinical decision-making in-patient selection prior to revascularization and treatment planning. We are also intended to share the initial outcome in available follow up cases.
J Inv Clin Cardiol 2023; 5(2): 45-52
Potential for material valorization of composites (glass fiber in polyester resin) in concrete: performance evaluation on mortar
Authors: Zambon Agnès , Deleglise-Lagardere Mylène 
Published: October 2024

IntroductionGlass fibers with polyester resin structural composites are highly sought after in many sectors such as transportation industries, thanks to their low density and fairly good mechanical properties. However, their end-of-life management is not yet satisfactory. Composites mostly end in energy recovery in the best-case scenario or, worse, in landfills. Transformation into shreds and powders for reuse as a new source of raw material for the construction sector (concrete) is an economically and environmentally attractive recovery solution. The present study investigates the development of a concrete filled with glass/polyester composite shreds.MethodsTo this end, rheological (cone spread) and physico-mechanical (density and mechanical strength in flexion and compression) characterization tests were carried out. Several mix designs were tested in order to understand the impact of introducing composite shreds as a substitute for sand. Composite shreds were introduced in the following ratios by volume: 0, 1, 1.5, 2, 2.5, 3, and 7% with water and cement ratio equal to 0.5, 0.6, and 0.7.Results and discussionThe results obtained indicate that workability decreases with the substitution of sand by shreds. For a substitution of sand by shreds of 2%, it is relatively small, and the pouring of the mortar is still feasible. The decrease can be attributed to the water absorption of the composite shreds. Concerning mechanical results, for formulations with a substitution percentage of composite shreds lower than 3%, the mechanical strength (both compression test and flexure test) is slightly higher than that of the reference sample. The increase in compressive strength that can be observed is at its maximum, equal to 10%, compared to that of the reference sample. These results are in line with density results, which are also slightly higher than that of the reference sample. This effect can be attributed to water absorption of composite shreds and the filling effect of the powders. For a percentage of substitution equal to 7%, the mechanical strength is lower than that of the reference sample (30% decrease), with a compressive strength equal to 33 MPa (47 MPa for the reference sample). For this percentage of substitution equal to 7%, a decrease in density is also observed (6% decrease) and can be explained by the porosity created by the incorporation of the composite shreds into the mortar.
Differences in cell subsets between sun-exposed and unexposed skin: preliminary single-cell sequencing and biological analysis from a single case
Authors: Zhou Feng , Sun Yu , Chen Xinling , Hou Wenyi , Shen Jing , Lai Wei , Han Kai , Zheng Yue 
Published: October 2024

IntroductionThe composition and subsets of skin cells continuously change in a dynamic manner. However, the specific microcosmic alterations of human photoaged skin, independent of chronologic aging, remain unclear and have been infrequently analyzed. This study aimed to evaluate the biological processes and mechanisms underlying cell-subgroup alterations in skin photoaging.MethodsWe utilized single-cell sequencing and biological analysis from a single case to investigate the effects of photoaging. Skin punch biopsies were taken from sun-exposed forearm skin and unexposed buttock skin from the same individual for comparative analysis.ResultsOur analysis identified 25 cell clusters and 12 skin cell types, revealing significant changes in unique gene expressions between the sun-exposed and unexposed skin samples. A comparison of cell numbers within each cluster revealed 9 dominant cell clusters in sun-exposed skin and 16 dominant cell clusters in unexposed skin. Enrichment analysis indicated that PD-L1 expression and the PD-1 checkpoint pathway were more prominent in sun-exposed skin, while MAPK, TNF-alpha, TGF-beta, and apoptosis pathways were more enriched in hair follicle cells of sun-exposed skin.DiscussionThis study reveals changes in cell components in photoaged skin from a single case and provides novel insights into cellular subpopulations and pathology during repeated UVA-induced skin damage. These findings enhance our understanding of the complex interplay between different cells in photoaged skin and offer potential targets for preventing human skin photoaging and UV-induced skin cancers.
Screening for probiotic properties and potential immunogenic effects of lactobacilli strains isolated from various food products
Authors: Kowalczyk Magdalena , Radziwill-Bienkowska Joanna M. , Marć Małgorzata A. , Jastrząb Rafał , Mytych Jennifer , Siedlecki Paweł , Szczepankowska Agnieszka K. 
Published: October 2024

IntroductionDeceleration of disease progression and re-establishment of microbial balance in the gut is often achieved by application of lactobacilli strains. Their beneficial effects are associated with probiotic properties, which may be accompanied by immunomodulatory action at mucosal surfaces.MethodsTo single out such strains, we screened almost three hundred lactobacilli isolates from eight genera and various food sources for acid and bile salt tolerance, adhesion to mucin as well as hemolytic activity and antibiotic susceptibility. Moreover, the immunomodulatory effects of cell-free supernatant (CFS) fractions of five lactobacilli strains were assessed using an in vitro cell line model.Results and discussionBy our rationalized selection approach, we identified thirty-five strains with probiotic potential and biosafety features. Additionally, we showed that CFS from the Lactiplantibacillus L_4 strain downregulates proinflammatory cytokines IL-8 and IL-1β. In contrast, IL-8 expression was found to increase after treatment with CFSs of Lactiplantibacillus L_2 and L_5 and IL-1β was upregulated by CFSs of Lactiplantibacillus L_1 and Lactiplantibacillus L_3. Overall, our result delineate a rational approach of selecting lactobacilli strains for probiotic development to support the gut microbiota equilibrium and reinforce the host immune system.
First-in-human phase 1 dose-escalation results with livmoniplimab, an antibody targeting the GARP:TGF-ß1 complex, as monotherapy and in combination with the anti–PD-1 antibody budigalimab in patients with advanced solid tumors
Authors: Shimizu Toshio , Powderly John , Abdul Razak Albiruni , LoRusso Patricia , Miller Kathy D. , Kao Steven , Kongpachith Sarah , Tribouley Catherine , Graham Michelle , Stoll Brian , Patel Maulik , Sahtout Mohammad , Blaney Martha , Leibman Rachel , Golan Talia , Tolcher Anthony 
Published: October 2024

BackgroundTransforming growth factor (TGF)-ß1 is a pleiotropic cytokine that can promote tumor growth and suppress antitumor immune responses. Latent TGF-ß1 associates with glycoprotein-A repetition predominant (GARP) on the surface of regulatory T cells prior to its activation and release. Livmoniplimab is a monoclonal antibody (mAb) that binds the GARP:TGF-ß1 complex to inhibit activation and release of TGF-ß1. It is in clinical development in combination with budigalimab, an anti-programmed cell death protein 1 Fc-modified mAb. The first-in-human, phase 1, dose-escalation results are presented herein (ClinicalTrials.gov: NCT03821935).MethodsThe dose-escalation phase enrolled adult patients with advanced solid tumors. Patients received escalating doses of livmoniplimab ranging from 3mg to 1500mg, once every 2 weeks (Q2W), as monotherapy or in combination with a 500mg fixed dose of budigalimab Q4W. The primary objective of the dose escalation was to determine the recommended phase 2 dose. Secondary objectives were to assess safety and pharmacokinetics (PK), and exploratory objectives included evaluating preliminary efficacy.ResultsFifty-seven patients enrolled in the dose escalation: 23 in monotherapy cohorts and 34 in combination therapy cohorts. Dose-limiting toxicities were limited, no maximum tolerated dose was reached, and the maximum administered dose of 1500mg was selected for dose expansion. The most common adverse events reported in monotherapy-treated patients were fatigue, anemia, and nausea, and those in combination therapy-treated patients were pruritus, fatigue, nausea, and anemia. Livmoniplimab exhibited dose-proportional PK, and peripheral blood biomarker data demonstrated saturation of the GARP:TGF-ß1 complex on platelets at livmoniplimab doses within the linear PK range. No objective tumor responses were observed in the monotherapy dose escalation. However, the objective response rate was 15% in the combination dose escalation, with a median response duration of 8.4 months.ConclusionLivmoniplimab was well-tolerated as monotherapy and in combination with budigalimab in the dose-escalation phase. Encouraging preliminary efficacy was demonstrated in the combination dose escalation in heavily pretreated patients, supporting further development of this novel drug combination in patients with advanced solid tumors.
A national survey of first line antibiotic use in neonatal units – and the potential scope for iatrogenic sensorineural hearing loss prevention
Authors: Peterson J. , Muddiman L. , Groves F. , Booth N. , Newman W. G. , McDermott J. H. , Mahaveer A. 
Published: October 2024

ObjectiveNational Institute for Clinical Excellence (NICE) guidance for the management of neonatal sepsis recommends a first-line antibiotic regimen containing an aminoglycoside (gentamicin). Aminoglycoside exposure causes sensorineural hearing loss in individuals with a specific mitochondrial genetic variant (m.1555A&gt;G). This variant can be detected promptly (in &lt;30 min) by a point of care test. NICE does allow for variation in antibiotic regimes depending on local microbiology guidance. As practices can vary, this survey aimed to determine the current use of first-line antibiotic agents within neonatal units and postnatal wards across the UK.Design and settingA telephone survey was conducted across all neonatal units in the United Kingdom. Responses were requested from a member of the neonatal team experienced in neonatal septic screening processes. One response was recorded per unit.ResultsOf the 187 neonatal units, 186 (99%) responded to the survey. One unit declined to participate. The survey results show most neonatal units (93%) and postnatal wards (74%) across the United Kingdom use aminoglycosides as first-line antibiotic agents. Antibiotic regimes varied between different units and between different locations within the same hospital (NICU vs. postnatal wards). In cases where there was a contraindication to aminoglycosides, the most common alternative antibiotic was cefotaxime.ConclusionsMost neonatal units in the UK use an aminoglycoside antibiotic as first-line agent for suspected sepsis. This places infants with the m.1555A&gt;G genetic variant at risk of iatrogenic hearing loss. There needs to be integration of point-of-care genetic testing within the neonatal septic screening pathway.
Comparison of plant microbiota in diseased and healthy rice reveals methylobacteria as health signatures with biocontrol capabilities
Authors: Oeum Kakada , Suong Malyna , Uon Kimsrong , Jobert Léa , Bellafiore Stéphane , Comte Aurore , Thomas Emilie , Kuok Fidero , Moulin Lionel 
Published: October 2024

IntroductionRice (Oryza sativa) is a staple food worldwide, but its production is under constant pressure from both abiotic and biotic stresses, resulting in high use of agrochemicals. The plant microbiome harbours microorganisms that can benefit plant health and provide alternatives to the use of agrochemicals. The composition of plant microbiomes depends on many factors (soil composition, age, and health) and is considered a primary driver of future plant health. To identify plant microbiomes that protect against disease, we hypothesised that asymptomatic rice plants in fields under high pathogen pressure (i.e., healthy islands of plants among predominantly diseased plants) might harbour a microbiota that protects them from disease.Material and MethodsWe sampled healthy and leaf-diseased plants in rice fields with high disease incidence in Cambodia and profiled their microbiota at leaf, root, and rhizosphere levels using 16S V3V4 and 18S V4 amplicon barcoding sequencing.ResultsComparison of amplicon sequence variants (ASV) of the microbiota of healthy and diseased samples revealed both disease and healthy signatures (significant enrichment or depletion at ASV/species/genus level) in both fields. The genera Methylobacterium and Methylorubrum were identified health taxa signatures with several species significantly enriched in healthy leaf samples (Methylobacterium indicum, Methylobacterium komagatae, Methylobacterium aerolatum, and Methylorubrum rhodinum). A cultivation approach on rice samples led to the isolation of bacterial strains of these two genera, which were further tested as bioinoculants on rice leaves under controlled conditions, showing for some of them a significant reduction (up to 77%) in symptoms induced by Xanthomonas oryzae pv. oryzae infection.DiscussionWe validated the hypothesis that healthy plants in fields under high disease occurrence can host specific microbiota with biocontrol capacities. This strategy could help identify new microbes with biocontrol potential for sustainable rice production.
KORELASI JUMLAH MONOSIT DENGAN KADAR C-REACTIVE PROTEIN PADA PENDERITA TUBERKULOSIS
Authors: Nuswantoro Ari , Astuti Fitri Mega , Hartati Fitri Ulya , Aditia Aditia 
Published: October 2024

Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) is still a public health problem throughout the world, including in Indonesia. Monocytes play a role in cellular reactions against MTB bacteria and are considered a marker of active TB spread. C-Reactive Protein (CRP) examination is very useful for diagnosing inflammation or infectious diseases. This study aimed to determine the correlation between monocyte counts and CRP levels in TB patients in North Putussibau. This research was observational analytic with cross-sectional approach and the sampling technique was accidental nonprobability sampling. The sample was TB patients in North Putussibau who were undergoing treatment for 0, 2, and 6 months in 2022, resulting in 36 patients. Monocyte counts were examined using the fluorescence method and CRP levels were examined using the latex agglutination method. At 0 months of treatment, the monocyte count was obtained with an average of 1.096.88cells/µL and average CRP level of 55.13mg/L, at 2 months of treatment the average monocyte count was 742.31 cells/µL and CRP levels were obtained an average of 10.62mg/L, and after 6 months of treatment an average monocyte count was 414.29cells/µL and an average CRP level of 0mg/L. The Kendall's Tau-b test showed a significant value 0.000 (<0.05), which means there was a significant correlation between the number of monocytes and CRP levels in TB patients with a correlation coefficient 0.645 which shows the strength of strong relationship. This shows that the decrease in the number of monocytes and CRP levels is in line with the length of the TB treatment period.
KNOWLEDGE, BEHAVIOR, EXPOSURE, AND PERCEPTION OF STRESS, ANXIETY, AND DEPRESSION AMONG HEALTH CENTER WORKERS DURING COVID-19
Authors: Prihanti Gita Sekar , Illahika Anung Putri , Hasna Alifah , Natiq Ikmalun , Hadiningtyas Kartika Lintang , Furkan Muhammad , Wicaksono Trio Rizal , Drestania Zhenna Vinenzha Linda 
Published: October 2024

Salah satu tantangan besar dalam pelayanan kesehatan yaitu krisis pandemi coronavirus disease (COVID-19). Petugas kesehatan di garis depan yang terlibat langsung dengan COVID-19 berisiko mengalami tekanan psikologis dan gejala kesehatan mental lebih tinggi ditambah dengan beban kerja yang semakin besar. Tujuan penelitian untuk mengetahui pengaruh pengetahuan, perilaku, paparan penyakit, persepsi risiko penyakit, dan ketakutan terhadap tingkat stres, kecemasan, dan depresi terkait COVID-19 pada petugas. Metode penelitian yang digunakan analitik observasional dengan desain cross sectional. Jumlah sampel 61 responden. Teknik sampling yang digunakan adalah total sampling. Sampel mengisi kuesioner dengan 61 item pertanyaan. Data dianalisis secara univariat untuk mengetahui karakteristik gambaran sampel, uji bivariat menggunakan uji chi-square untuk mengetahui hubungan antar variabel bebas terhadap variabel terikat, uji multivariat untuk mengetahui variabel bebas yang paling berpengaruh terhadap tingkat stres, kecemasan dan depresi. Hasil pada uji multivariat menunjukkan hubungan antara jenis kelamin perempuan dan tingkat stres (p = 0,018; OR = 14.026; CI = 1.564–125.741); terpapar terhadap tingkat stres (p = 0,012; OR = 16.319; CI = 1.848–144.095); dan persepsi risiko yang tinggi terhadap tingkat kecemasan (p = 0,003; OR = 25.288; CI = 2.953–216.543). Kesimpulan penelitian ini adalah terdapat faktor signifikan yang mempengaruhi tingkat stres petugas pada masa COVID-19, yaitu jenis kelamin, paparan, persepsi risiko penyakit dan ketakutan mempengaruhi tingkat kecemasan petugas.
NGHIÊN CỨU XÂY DỰNG QUY TRÌNH BÀO CHẾ VIÊN NANG CỨNG ENTVN CHỨA PROBIOTIC ENTEROCOCCUS FAECIUM
Authors: Anh Bùi Thị Lan , Giang Hoàng , Mạnh Đào Nguyên 
Published: October 2024

Nghiên cứu tiến hành xây dựng quy trình bào chế viên nang cứng EntVN từ các chủng lợi khuẩn Enterococcus faecium F26BA và F54BA được phân lập từ nguồn thực phẩm Việt Nam. Nồng độ sữa tiệt trùng để tạo sinh khối giống khởi động là 15% (w/w), thời gian tiến hành đông khô sinh khối ướt là 24 giờ, tỷ lệ tá dược độn (bột đậu nành) được trộn với sinh khối probiotic 1:4 (sinh khối:tá dược, w/w), nhiệt độ sấy 40oC trong 4 giờ thu được bột sinh khối probiotic có mật độ khuẩn lạc đạt 4,5x1010CFU/g. Bột sinh khối (460 mg/viên nang) sau khi nghiền mịn trộn với tá dược độn (20 mg/viên nang), tá dược dính (10 mg/viên nang), tá dược trơn chảy (10 mg/viên nang), tiến hành đóng nang. Viên nang EntVN thu được đạt các chỉ tiêu về khối lượng (sai số không quá 7,5%), độ rã (dưới 30 phút), các chỉ tiêu về giới hạn nhiễm khuẩn, hàm lượng kim loại nặng và lượng khuẩn lạc (3,3x1010CFU/g). Viên nang EntVN được bào chế từ các chủng lợi khuẩn bản địa với các đặc điểm dễ dàng thích nghi và phù hợp với người Việt, nguyên liệu bào chế phổ biến, quy trình đơn giản, giá thành vừa phải đáp ứng được nhu cầu thị trường Việt Nam.