bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antagonistic anticancer effect of paclitaxel and digoxin combination
Authors: Pereira Duane Gischewski , Rendeiro Mariana Manzano , Cortes Vanessa Faria , Barbosa Leandro Augusto , Quintas Luis Eduardo M. 
Published: March 2019

AbstractDespite the growing interest in the antitumor effect of cardiotonic steroids, combination treatments with well‐established chemotherapy drugs like paclitaxel have been rarely investigated. Moreover, paclitaxel has been suggested as a Na+/K+‐ATPase inhibitor. Here we investigated the effect of paclitaxel and digoxin alone or in combination on the viability of human lung (A549) and cervical cancer (HeLa) cell lines and the inhibitory effect of paclitaxel on several mammalian Na+/K+‐ATPases. Although the viability of both tumor cell lines was concentration‐dependently affected by digoxin treatment after 48 hours (A549 IC50 = 31 nM and HeLa IC50 = 151 nM), a partial effect was observed for paclitaxel, with a maximal inhibitory effect of 45% at 1000 nM with A549 and around 70% with HeLa cells (IC50 = 1 nM). Although the two drugs were cytotoxic, their combined effect in HeLa cells was revealed to be antagonistic, as estimated by the combination index. No direct inhibitory effect of paclitaxel was detected in human, pig, rat, and mouse Na+/K+‐ATPase enzymes, but high concentrations of paclitaxel decreased the Na+/K+‐ATPase activity in HeLa cells after 48 hours without affecting protein expression. Our findings demonstrate that, under our conditions, paclitaxel and digoxin cotreatment produce antagonistic cytotoxic effects in HeLa cells, and the mechanism of action of paclitaxel does not involve a direct inhibition of Na+/K+‐ATPase. More studies shall be designed to evaluate the consequences of the interaction of cardiotonic steroids and chemotherapy drugs.
microRNA‐329 suppresses epithelial‐to‐mesenchymal transition and lymph node metastasis in bile duct cancer by inhibiting laminin subunit beta 3
Authors: Liao Chun‐Hong , Liu Yi , Wu Yi‐Fei , Zhu Si‐Wei , Cai Rong‐Yao , Zhou Lei , Yin Xin‐Min 
Published: March 2019

AbstractBile duct cancer (BDC), also known as cholangiocarcinoma, is a highly desmoplastic cancer with a growth pattern characterized by periductal extension and infiltration. Studies have suggested that microRNAs (miRNAs) play an important role in BDC progression. Here we aim at investigating the effects of miR‐329 on BDC development, focusing especially on epithelial‐to‐mesenchymal transition (EMT) in vitro and lymph node metastasis in vivo. Expression microarrays associated with BDC tissues were collected and differentially expressed genes were analyzed, followed by miRNA target prediction and verification. The role miR‐329 played in BDC was examined using gain‐of‐function and loss‐of‐function methods. The expressions of miR‐329, laminin subunit beta 3 (LAMB3), and EMT markers, in addition to cell proliferation, migration, and invasion were evaluated. Furthermore, nude mice models of BDC were established to observe tumor growth and metastatic lymph nodes. The LAMB3 was identified as an upregulated gene based on the GSE77984 and GSE45001 microarray analysis. LAMB3 was also predicted and confirmed to be a target gene of miR‐329 by dual‐luciferase reporter assay. Through further cell experiments, the EMT process was reversed, cell proliferation, invasion, and migration were suppressed, when miR‐329 was upregulated. Furthermore, in vivo experiments exhibited that the overexpression of miR‐329 inhibited tumor growth and the number of metastatic lymph nodes. This study provides in vivo and in vitro evidence that miR‐329 inhibits BDC progression through translational repression of LAMB3. Therefore, the obtained results may aid as an experimental basis for improving prognosis of BDC.
Phytoextraction technologies for mercury‐ and chromium‐contaminated soil: a review
Authors: Ranieri Ezio , Moustakas Konstantinos , Barbafieri Meri , Ranieri Ada C , Herrera‐Melián José A , Petrella Andrea , Tommasi Franca 
Published: March 2019

AbstractMercury and chromium represent two important elements of environmental concern and with a strong impact on human health. Several technologies are available to clean up the environment from these kinds of contaminants, but most of them are costly and difficult to use to get optimum results. Currently, phytoextraction is an effective and affordable technological solution used to extract or remove metals from contaminated soil and water by the use of plants as tools. This technology is environment‐friendly and potentially cost‐effective. This paper aims to compile some information about the two metals mercury and chromium, their effects and their possible treatment by phytoextraction, a branch of phytoremediation technologies aimed to confront metal/metalloid pollution. © 2019 Society of Chemical Industry
Multi‐residue analysis of 44 pharmaceutical compounds in environmental water samples by solid‐phase extraction coupled to liquid chromatography‐tandem mass spectrometry
Authors: Miossec Carole , Lanceleur Laurent , Monperrus Mathilde 
Published: March 2019

AbstractA solid‐phase extraction combined with a liquid chromatography‐tandem mass spectrometry analysis has been developed and validated for the simultaneous determination of 44 pharmaceuticals belonging to different therapeutic classes (i.e., antibiotics, anti‐inflammatories, cardiovascular agents, hormones, neuroleptics, and anxiolytics) in water samples. The sample preparation was optimized by studying target compounds retrieval after the following processes: i) water filtration, ii) solid phase extraction using Waters Oasis HLB cartridges at various pH, and iii) several evaporation techniques. The method was then validated by the analysis of spiked estuarine waters and wastewaters before and after treatment. Analytical performances were evaluated in terms of linearity, accuracy, precision, detection, and quantification limits. Recoveries of the pharmaceuticals were acceptable, instrumental detection limits varied between 0.001 and 25 pg injected and method quantification limits ranged from 0.01 to 30.3 ng/L. The precision of the method, calculated as relative standard deviation, ranged from 0.3 to 49.4%. This procedure has been successfully applied to the determination of the target analytes in estuarine waters and wastewaters. Eight of these 44 pharmaceuticals were detected in estuarine water, while 26 of them were detected in wastewater effluent. As expected, the highest values of occurrence and concentration were found in wastewater influent.
Do people who travel more perform better at work?
Authors: Miyakawa Erika , Kawakubo Atsushi , Oguchi Takashi 
Published: March 2019

AbstractIn this study, we examined how travel experiences affect job performance by improving generic skills and self‐efficacy. We collected data using a questionnaire delivered to 232 Japanese employees working for a temporary employment agency. Additionally, an objective measurement (financial data) was used to assess employees' job performance level. The results revealed that travel experiences positively influenced job performance as mediated by generic skills and general self‐efficacy. In addition to offering important implications for organizations and employees, our findings are likely to advance the current research on the mechanism of the benefits of travel on job performance.
Utilization and reimbursements for sinus procedures: A five‐year analysis
Authors: Koester Lindsey K. , Goyal Parul 
Published: March 2019

Objectives/HypothesisBalloon sinuplasty has become an increasingly popular option for patients undergoing surgical treatment of sinusitis. This study analyzes utilization and growth of these procedures across a large cohort of patients over a 5‐year period.Study DesignRetrospective data review.MethodsThis was a retrospective review of Medicare utilization and billing data. Utilization and payment values were obtained from Medicare claims data using Centers for Medicare and Medicaid Services datasets. All Medicare claims were analyzed from 2012 to 2016. Data were extracted for balloon sinuplasty and endoscopic sinus surgery claims. Procedure location, total submitted claims, charges, and payments were compared.ResultsFrom 2012 to 2016, the number of balloon procedures increased from 5,603 to 25,640. Traditional endoscopic sinus surgery procedures increased from 15,509 to 18,164. Aggregate Medicare payments to otolaryngologists for endoscopic sinus surgery have remained relatively stable, whereas there has been a 450% increase in total payments to providers of balloon sinuplasty. In 2016, total payments to providers of balloon procedures ($40.5 million) were substantially higher than payments to providers of non–balloon‐based endoscopic sinus surgery ($4.7 million). The number of providers performing balloon procedures has increased 277% versus 17% for traditional sinus surgery.ConclusionsThere has been a rapid expansion in the number of sinus procedures in the Medicare population from 2012 to 2016. Office‐based balloon procedures account for the overwhelming majority of the increases in procedures and payments. Per procedure and aggregate payments are now higher for sinuplasty procedures than for traditional sinus surgery.Level of EvidenceNALaryngoscope, 129:2224–2229, 2019
Soil loss: Effect of plant litter incorporation rate under simulated rainfall conditions
Authors: Wang Lunjiang , Zhang Guanghui , Wang Xue , Zhu Pingzong 
Published: March 2019

AbstractPlant litter can control land degradation by increasing soil organic matter and decreasing soil erosion by covering the soil surface or being incorporated into topsoil. However, the effect of litter incorporation on soil erosion is not clear. This study aimed to investigate the effect of litter incorporation into topsoil on interrill erosion. The poorly decomposed litter was collected from the field and incorporated into topsoil (5 cm) at six rates (ranging from 0 to 0.5 kg m−2). Laboratory rainfall (80 mm hr−1) experiments were conducted under five slope gradients (ranging from 9% to 47%). The results showed that the soil loss rates declined exponentially with increase in litter incorporation rate on slope gradients between 9% and 36%. However, on slope gradient of 47%, litter incorporation caused an increase in the soil loss rate. The efficiency of litter incorporation in controlling soil loss varied from −21.7% to 40.5% with a mean of 10.8%. It decreased linearly with the slope gradient (R2 = 0.34). The decrease can be explained by two simultaneous but opposite effects: the effect of preventing erosion and that of promoting erosion. The former was induced by decreases in the effective erosive force of raindrop and overland flow exerted on soil, and soil erodibility, along with increases in sediment interception. The latter was caused by rapid saturation of the surface soil and concentrated overland flow. The results demonstrate the complexity of the effect of litter incorporation on soil erosion and can improve our understanding of litter in soil loss control.
Feelings and functionality in social networking communities: A regulatory focus perspective
Authors: Krishen Anjala S. , Berezan Orie , Raab Carola 
Published: March 2019

AbstractA sense of community is vital to the sustainability of both face‐to‐face and virtual communities. In this study, we use a mixed methods design to study the motivations that lead to satisfaction in a social media network. In Study 1, we semantically analyze the qualitative comments from a survey regarding the reasons for using social networks (n = 237). Consistent with the literature, the results identify two categories of underlying motivations—hedonic (feelings) and utilitarian (functionality). With these results, we apply regulatory focus theory in Study 2 to propose a structural equation model (
n = 622). This model differentiates between the experiences of promotion‐ and prevention‐focused individuals with social networking based on the core elements of feelings versus functionality
. Our findings indicate that a sense of belonging and emotional connection are key hedonic elements and are more relevant for promotion‐focused individuals. For prevention‐focused individuals, the utilitarian and functional aspects of interactivity (influence) and innovativeness (risk) are more important in influencing satisfaction with a social network. Overall, positive and innovative user experience with a social media network requires the creation of an emotional connection, the existence of interactivity between members, and the cultivation of a sense of belonging.
Prevalence of bee viruses in Apis cerana cerana populations from different locations in the Fujian Province of China
Authors: Hassanyar Aqai Kalan , Huang Shaokang , Li Zhiguo , Rizwan Muhammad , Mehmood Shahid , Raza Muhammad Fahad , Qasim Muhammad , Hussain Mubasher , Su Songkun 
Published: March 2019

AbstractPrevalence of honeybee viral diseases has recently been causing major problems in the beekeeping industry, causing economic losses worldwide. Honeybees are susceptible to a variety of diseases and various pathogens. Among these pathogens, prevalence viruses, along with other factors, are seriously threatening the health of bee species. In the present study, samples were collected from 80 Apis cerana cerana (A. c. cerana) colonies from three different locations, Cangshan, Fuan, and Yongtai, in the Fujian Province of China. All samples were screened using the reverse transcription polymerase chain reaction (RT‐PCR) method for detection of seven honeybee viruses, namely, Chinese sacbrood virus (CSBV), deformed wing virus (DWV), Israeli acute paralysis virus (IAPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV), and Kashmir bee virus (KBV). Our results showed that CSBV was the most prevalent as it was detected in (90%), of the samples, DWV was detected in (81.25%), and IAPV was detected in (26.25%). In contrast, insignificant prevalence results were obtained from all apiaries for BQCV, CBPV, APBV, and KBV, which were not detected in any sample. Here, we are providing the first report on the molecular detection of honeybee viruses, especially the prevalence of IAPV, from different regions in the Fujian Province of China with a high prevalence of bee viruses, on A. c. cerana, and there is great concern for the presence of honeybee viruses in the population of the native honeybee (A. c. cerana) in China.
Novel mutations found in the ATP7B gene in Chinese patients with Wilson's disease
Authors: Qian Zhiling , Cui Xiongwei , Huang Yunli , Liu Yanmin , Li Ning , Zheng Sujun , Jiang Jun , Cui Shichang 
Published: March 2019

AbstractBackgroundWilson's disease (WD) is an autosomal recessive genetic disease caused by mutations in ATP7B and characterized by copper metabolism disorders.MethodsDirect sequencing of the ATP7B gene is the most sensitive and widely used confirmatory testing method. Fourteen probands with WD and 12 family members participated in this study. The ATP7B gene was analyzed by direct sequencing.ResultsTwenty‐nine different variants (27 substitutions, 1 duplication, 1 deletion) were found. Of the 23 reported variants, nine nondisease variants, 11 disease variants, one silent variant, and two variants with uncertain functions were identified. The six novel variants included c.1875T>A, c.2306T>C, c.3028A>G, c.3243G>A, c.3437_3438 delTG, and c.3903+5G>A.ConclusionThese findings will assist in the diagnosis of WD. The novel variants have enriched the WD database.
Comparison of high flow nasal cannula with noninvasive ventilation in chronic obstructive pulmonary disease patients with hypercapnia in preventing postextubation respiratory failure: A pilot randomized controlled trial
Authors: Jing Guoqiang , Li Jie , Hao Dong , Wang Tao , Sun Yunliang , Tian Huanhuan , Fu Zhong , Zhang Yuewei , Wang Xiaozhi 
Published: March 2019

AbstractHigh flow nasal cannula (HFNC) has been shown to improve extubation outcomes in patients with hypoxemia, but the role of HFNC in weaning patients with chronic obstructive pulmonary disease (COPD) with hypercapnia from invasive ventilation is unclear. We compared the effects of HFNC to noninvasive ventilation (NIV) on postextubation vital signs and arterial blood gases (ABGs) among patients with COPD. Other outcomes included comfort scores, need for bronchoscopy, use of pulmonary medications, and chest physiotherapy. Forty‐two COPD patients who had persistent hypercapnia at extubation were assigned randomly to receive HFNC (22) or NIV (20). Twenty patients in each group were enrolled for per‐protocol analysis with regard to primary outcomes. Vital signs and ABGs before extubation were similar between groups. At 3 hr after extubation, pH in the NIV group was lower than HFNC group (7.42 ± 0.06 vs. 7.45 ± 0.05, p = 0.01). At 24 hr after extubation, patients’ mean arterial pressure (82.97 ± 9.04 vs. 92.06 ± 11.11 mmHg, p = 0.05) and pH (7.42 ± 0.05 vs. 7.46 ± 0.03, p = 0.05) in the NIV group were lower than in the HFNC group. No significant differences were found at 48 hr after extubation. In the HFNC group, comfort scores were better (3.55 ± 2.01 vs. 5.15 ± 2.28, p = 0.02) and fewer patients needed bronchoscopy for secretion management within 48 hr after extubation (2/22 vs. 9/20, p = 0.03). HFNC is a potential alternative to NIV to wean hypercapnic COPD patients with regard to vital signs and ABGs, HFNC improved patients’ comfort and secretion clearance.
Tribal Gaming and Educational Outcomes in the Next Generation
Authors: Thompson Owen 
Published: March 2019

AbstractFollowing a series of federal policy changes and court rulings in the late 1980s, over 400 casinos owned by Native American tribes were opened throughout the United States, and expanded tribal gaming has transformed the economic development trajectories of many American Indian tribes. While most existing evaluations of tribal gaming's impacts focus on contemporaneous effects, the present paper evaluates whether the advent of tribal casinos affected educational outcomes in the subsequent generation of American Indian children. I assemble data on the adult educational outcomes of 11,647 American Indians across 36 counties who were children when a local casino was opened. I use these data to compare individuals who were relatively young at the time of a casino opening, and therefore had greater exposure to post‐gaming socioeconomic conditions, and individuals who were from the same county but were relatively old at the time of a casino opening, while also exploiting differences in the relative sizes of tribal gaming operations. Using this approach, I estimate that exposure to an average‐sized gaming operation during childhood increased adult educational attainment by .328 years and increased the probability of high school graduation and post‐secondary degree completion by 5 to 14 percent. No substantive improvements in adult educational outcomes are observed among white children from the same counties. The magnitudes of the estimates imply a relationship between family income during childhood and educational achievement that is similar to previous research within other low‐income populations.
Bile ductular reactions in the liver: similarities are only skin deep
Authors: Alison Malcolm R , Lin Wey‐Ran 
Published: March 2019

AbstractExtensive bile ductular reactions (DRs) accompany many cholestatic liver diseases such as primary biliary cholangitis and primary sclerosing cholangitis (PSC) as well as parenchymal liver cell diseases such as alcoholic liver disease, non‐alcoholic steatohepatitis and HCV and HBV infections. DRs originate from bile ducts or hepatocytes after damage and can be identified by expression of markers associated with cholangiocytes, often being associated with disease progression and fibrosis. In a recent issue of The Journal of Pathology, Govaere et al employed high‐throughput RNA sequencing to compare the transcriptomic profiles of DR cells from liver diseases of different aetiology; HCV infection affecting hepatocytes and PSC initially affecting biliary epithelial cells. Both DR transcriptomes were markedly different from that of their neighbouring hepatocytes and 330 genes were significantly differently expressed between the DRs of the HCV and PSC liver diseases. Exploring such gene expression profiles could enable therapeutic targeting of DRs, on the one hand to inhibit liver fibrosis and inflammation and conversely to promote hepatocyte and cholangiocyte regeneration. Copyright © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Linkage of the CF foundation patient registry with the pediatric health information system database
Authors: Cogen Jonathan D. , Hall Matt , Loeffler Deena R. , Gove Nancy , Onchiri Frankline , Sawicki Gregory S. , Fink Aliza K. 
Published: March 2019

AbstractIntroductionThe Cystic Fibrosis Foundation Patient Registry (CFFPR) contains clinical and demographic data from ∼85% of US cystic fibrosis (CF) patients across 120 care centers, but lacks robust inpatient hospitalization data. In contrast, the Pediatric Health Information System (PHIS) database includes inpatient clinical and resource utilization data from 49 US children's hospitals. The creation of a linked CFFPR‐PHIS dataset can uniquely address questions related to in‐hospital pediatric CF treatment and management. We assessed the feasibility of linking the CFFPR and PHIS databases and determined if successfully linked CF patients were generalizable to unlinked patients.MethodsCF patients ≤21 years were eligible for linkage. The CFFPR and PHIS databases were linked at the patient level using indirect identifiers in a stepwise, deterministic, linkage approach. A validation cohort was created using a subset of patients to determine linkage accuracy. Clinical and demographic characteristics between linked and unlinked patients were compared to determine generalizability of the linked cohort.ResultsOf the 11 735 CF patients eligible for linkage from January 1st, 2005 through December 31st, 2016, 10 660 (91%) were successfully linked. Results of our single center validation cohort illustrated 100% accuracy. When compared to unlinked CF patients, fewer linked patients were born before 1990, more were Hispanic, and more were from West‐affiliated PHIS hospitals. Otherwise, no clinically meaningful differences were seen between linked and unlinked CF patients.ConclusionsWe demonstrated successful linkage of the CFFPR and PHIS databases, and created a large generalizable pediatric CF cohort for use in CF‐related research.