bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of minimally invasive Pouch technique with multilayered Leukocyte Platelet Rich Fibrin versus non-invasive Hyaluronic acid gel injection in the reconstruction of Interdental Papilla in the Esthetic Zone: A Randomized Clinical Trial
Authors: Alleithy Aya , Abuel-Ela Hala , Sarhan Susan 
Published: November 2024

Abstract

Background
Interdental papilla deficiency is of high concern from the esthetic and functional perspectives. Several invasive approaches have been reported for interdental papilla reconstruction. However, the long-term success of invasive approaches is still controversial, therefore this study aimed to evaluate the efficacy of Hyaluronic acid gel injection compared to multilayered leukocyte platelet-rich fibrin in reconstruction of deficient interdental papillae.
Methods
In this randomized clinical, double-blinded study, twenty patients with deficient interdental papilla class I or II in the esthetic zone were selected, then patients were randomized into two equal groups: group A (Multilayered L-PRF) and group B (HA gel). Sample size calculation and power analysis was designed to have adequate power to apply a 2-sided statistical test of the research hypothesis that there is no difference between groups. The predicted sample size (n) was a total of (20) cases. i.e. (10) for each group. The clinical and radiographic parameters were assessed at 3 and 6 months, As well as evaluation of patient satisfaction between the two tested groups.
Results
Both groups showed significant improvement in all clinical parameters at 3 months and 6 months postoperatively. Assessment of patient satisfaction showed overall improvement in both groups after 6 months, with successful reduction of the black triangle height.
Conclusions
Both injectable HA and multilayered L-PRF gave successful results and can be safe adjuncts to the invasive surgical techniques.
Trial registration:
The study was retrospectively registered in an appropriate registry and has been identified with the registration number (NCT05953896) with the date of registration (19-7-2023).
Long-Term Control of Recurrent Metastatic Castrate Sensitive Prostate Cancer with Radiotherapy: A Case Report.
Authors: Grewal Shan , Gouveia Andre , Hu Spencer , Tsakiridis Theodoros 
Published: November 2024

Abstract

Background:
Localized prostate cancer (PC), one of the most diagnosed cancers among men, has a relatively favorable prognosis; Metastatic PC, however, has significantly lower survivorship. Standard treatments for metastatic PC include androgen deprivation therapy (ADT), but this treatment diminishes the patient’s quality of life, and frequently progresses to castration-resistant disease. There is therefore interest in determining when ADT can be postponed or avoided. Stereotactic body radiation therapy (SBRT) has shown promise in managing the oligometastatic disease setting.
Case Presentation:
This report describes a patient with metastatic PC who underwent a total of nine rounds of SBRT with limited ADT. Initially diagnosed in 2012 with pT3a prostate adenocarcinoma, the patient was treated with surgery and radiation without ADT. The first PSA relapse occurred 6 years post-diagnosis with metastatic foci in the rib cage and spine. The patient refused systemic ADT and opted for local palliative radiotherapy with successful clearance. Novel metastatic foci were identified 10 months later, again treated with SBRT alone for a rapid biochemical response. PSA steadily rose until July 2022 when imaging revealed the spinal metastasis recurred plus novel nodal positivity. Finally, ADT was administered alongside SBRT, resulting in undetectable PSA levels by September 2022. The patient has since maintained biochemical remission with eugonadal testosterone levels.
Conclusions:
While ADT is crucial for metastatic PC, its adverse effects necessitate alternative strategies. This case demonstrates the potential of metastasis-directed radiotherapy like SBRT in managing metastatic lesions, delaying or reducing the need for ADT.
Bone marrow versus peripheral blood stem cells as the graft source for allogeneic transplantation from HLA-matched relative donors in adult T-cell leukemia/lymphoma: A nationwide retrospective study by the ATL working group of the Japan Society for Transplantation and Cellular Therapy
Authors: Itonaga Hidehiro , Fukushima Takuya , Kato Koji , Muranushi Hiroyuki , Tokunaga Masahito , Fukuda Takahiro , Kawakita Toshiro , Kato Takeharu , Mori Yasuo , Nakachi Sawako , Suehiro Youko , Kuriyama Takuro , Fujioka Machiko , Onizuka Makoto , Ichinohe Tatsuo , Ohbiki Marie , Atsuta Yoshiko , Fuji Shigeo , Yoshimitsu Makoto 
Published: November 2024

Abstract

Peripheral blood stem cells (PBSC) or bone marrow (BM) is selected as the graft source in the setting of allogeneic hematopoietic stem cell transplantation from HLA-matched related donors. To clarify the prognostic impact of graft sources in patients with adult T-cell leukemia/lymphoma (ATL), we performed a retrospective study using a propensity score analysis. In the entire population, 124 and 274 patients received transplantation with BM and PBSC, respectively. In the inverse probability of treatment weighting method, BM transplantation achieved comparable overall survival (OS)(P=0.593), chronic graft-versus-host disease (GVHD)- and relapse-free survival (CRFS)(P=0.317), and GVHD- and relapse-free survival (P=0.569) to PBSC transplantation. In the propensity score-matched subset (BM, n=120; PBSC, n=120), no significant differences were observed in OS (P=0.350), the incidence of relapse (P=0.335), or non-relapse mortality (P=0.924). PBSC transplantation showed faster neutrophil engraftment (P<0.001) and a higher incidence of severe acute GVHD (P=0.006) than BM transplantation. The development of grade I-II acute GVHD was associated with better OS in both BM (P=0.001) and PBSC transplantation (P=0.009), indicating the benefit of acute GVHD-associated graft-versus-ATL effects. The present study demonstrated that BM grafts provided a curative outcome along with graft-versus-ATL effects, similar to PBSC grafts, when patients underwent transplantation from HLA-matched related donors.
LSD1 is a targetable vulnerability in gastric cancer harboring TP53 frameshift mutations
Authors: Wang Suzeng , Yang Chunyu , Tang Junhui , Wang Kaiqing , Cheng Hao , Yao Surui , Huang Zhaohui , Fei Bojian 
Published: November 2024

Abstract

Background
TP53 mutations are linked to aggressive progression and chemoresistance in gastric cancer (GC). Frameshift mutation is the second most common mutation type of TP53. However, the consequences of this mutation type in GC were not well understood, and targeted therapies for cancer patients harboring frameshift mutations were also not established. Histone methylation significantly influences tumorigenesis in TP53-mutated cancers, and related inhibitors are emerging as specific therapeutic strategies.
Methods and Results
By treating GC cell lines harboring various TP53 mutation types with a library of histone demethylase inhibitors, we identified that GSK690, a reversible inhibitor of lysine-specific demethylase 1 (LSD1), selectively inhibits GC cells harboring TP53 frameshift mutations without nuclear localization sequence (NLS) (termed TP53 Frameshift NLS), which accounts for 89% TP53 frameshift mutations in GC patients. GSK690 showed significant specific inhibition in vitro and in vivo against this subtype by promoting G1/S cell cycle progression via inhibiting the LSD1-CCNA2 axis. Importantly, dual-luciferase assays and ChIP-qPCR confirmed that the loss of transcriptional repression activities of p53 in drives LSD1 upregulation in TP53 Frameshift NLS cancer cells.
Conclusions
In summary, our results indicate that the nuclear localization deficiency of p53 accounts for increased expression of LSD1 in TP53 Frameshift NLS GCs. GSK690 inhibits cell cycle progression and tumor growth by suppressing aberrantly activated LSD1-CCNA2 signaling in this GC subtype, counteracting malignant proliferation and thereby providing a precise therapeutic strategy for GC patients with TP53 Frameshift NLS.
Highly sensitive and selective detection of Hg2+ ions and antibacterial activity using a Schiff-base derivative
Authors: Alnajeebi Afnan M. , Yahya Rana , Shafie Alaa , Ashour Amal Adnan , Felemban Mohammed Fareed , Tayeb Faris J 
Published: November 2024

Abstract

A simple and highly effective Schiff-base fluorescent chemosensor (S1) was synthesized and characterized by 1HNMR and fluorescence spectroscopy. The synthesized chemosensor was applied for the selective and sensitive detection of Hg2+ ions. The chemosensor exhibited a strong 'turn-on' fluorescence response in a CH3OH/H2O (1:9, v/v) solution due to complex formation (S1-Hg2+) which block photo induce electron transfer (PET). The chemosensor showed significant sensitivity with very low detection limit of 0.002 ppm, making it suitable for trace-level Hg2+ detection. Furthermore, the S1-Hg2+ complex demonstrated excellent antibacterial activity against various Gram-positive and Gram-negative bacterial strains, broadening its utility beyond sensing. This dual-functional system offers significant potential in environmental and biomedical applications.
Biomechanics of cage for Stand-alone extreme lateral interbody fusion
Authors: Li Xiaohua , Gao Shangju , Li Wenyi , Wang Shuai , Li Ting 
Published: November 2024

Abstract

Background: To analyze the biomechanics of Stand-alone eXtreme lateral interbody fusion (Standalone XLIF) cage using finite element analysis.
Method: The L3-5 CT image data of a healthy adult male volunteer was selected to establish a normal lumbar vertebrae finite element model. Fix the lower edge of the L5, apply 500N pressure on the upper edge of the L3, and apply a torque of 7.5Nm, calculate the range of motion (ROM) of L4-5 under flexion, extension, left bending, right bending, left rotation, and right rotation.Then, the L4-5 intervertebral discs were excised, and the cage was implanted to establish a Stand-alone XLIF model. On this basis, bilateral pedicle screw fixation (BPSF) was added to establish the BPSF model. Analyze the ROM of L4-5 and the maximum stress of cage under all motion patterns.
Results: The ROM of L4-5 segments in the validation model under various motion states was basically consistent with previous reports. The lumbar finite element model was validated effectively. The range of activity of BPSF in each working condition was significantly lower than that of Normal and Stand-alone. Compared with BPSF, Normal and Stand-alone increase by 925.9%,1034.6% and 1107.4%,1146.2% in flexion and extension, respectively. Stand-alone had higher range of activity than Normal in flexion and extension, but lower in left bending, right bending, left rotation and right rotation. The maximum stress of stand-alone cage was greater than that of BPSF under all working conditions, and increased by 363.2% and 1899.4% in flexion and extension respectively compared with BPSF.
Conclusions: Stand-alone XLIF is poor in maintaining the stability of the fusion segment, and there is a higher risk of cage subsidence in the later stage.
T-shaped dysmorphic uterus: discrepancies between current 3D-ultrasound diagnostic criteria
Authors: Monaco Giulia , Nocita Elvira , Selntigia Aikaterini , Russo Consuelo , Farsetti Daniele , Reppuccia Sabrina , De Angelis Carlo , Zizolfi Brunella , Sardo Attilio Di Spiezio , Exacoustos Caterina 
Published: November 2024

Abstract

Purpose
Review and compare current classifications for diagnosing T-shaped uterus using three-dimensional transvaginal ultrasound (3D-TVS) measurements, identifying measurements that best correlate with the diagnosis.
Methods
This retrospective cohort study analyzed diagnostic measurements in patients with T-shaped uterus who underwent 3D-TVS at the University of Rome 'Tor Vergata' from 2016 to 2022. Of 7,588 patients, four sonographers re-evaluated 3D-TVS images of 72 initially diagnosed cases. Uterine morphology was assessed in the coronal plane by measuring fundal cavity width (R0), corpus-isthmic cavity width (Wi), lateral indentation angle (AI), lateral bulging (LB), T-angle (AT), fundal/isthmic cavity width ratio (R0/Wi), and the length of intracavitary line parallel to interostial line (R10). All cases were confirmed by hysteroscopy, excluding ambiguous findings.
Results
Of 72 initially evaluated patients, 50 met the inclusion criteria for final analysis. These patients had consistent 3D-TVS diagnoses from four sonographers and hysteroscopic confirmation from two experts. The combination of three CUME criteria (AT ≤ 40°, AI ≤ 130°, LB ≥ 7mm) identified only 8% of T-shaped uteri. Notably, 30 patients (60%) had an R10 measurement of ≤ 10mm. Additionally, 31 uteri (62%) met all three criteria: LB ≥ 5mm, AI ≤ 140°, and R0/Wi ≥ 5. Overall, 48 uteri (96%) satisfied at least two criteria. The study concluded that LB, R10, and R0/Wi are independent predictors of T-shaped uterus.
Conclusions
Significant discrepancies exist among current classifications for diagnosing T-shaped uterus. This study identified LB, R10, and R0/Wi as key parameters for accurate diagnosis. These measurements provide a precise and objective approach, aiding in the evaluation of the anomaly's impact on reproductive outcomes and the benefits of hysteroscopic treatment.
Outcomes and Prognostic Factors in Patients with Combined Severe Traumatic Brain Injury and Abdominal Trauma: A Retrospective Observational Study
Authors: Jung Hohyung , Heo Inhae , Jung Kyoungwon 
Published: November 2024

Abstract

Purpose
Severe traumatic brain injury (TBI) is a leading cause of death and disability. Approximately one-third to one-half of cases of TBI are associated with severe extracranial injuries, particularly abdominal trauma. This combination is associated with high mortality rates. However, studies describing the outcomes and prognostic factors of patients with combined severe head and abdominal trauma are lacking. Therefore, this study aimed to compare the clinical characteristics and outcomes and identify prognostic factors in this patient population.
Methods
This single-centre, retrospective observational study included patients with major trauma, TBI, and abdominal injury admitted to a Level 1 trauma centre between 1 January 2016 and 31 December 2020. The primary outcomes were in-hospital mortality and Glasgow Outcome Scale scores.
Results
Of 4,554 patients screened, 160 met the inclusion criteria. The in-hospital mortality rate was 20.6%, and 63.1% of the patients had favourable functional outcomes. Multivariate analysis identified that initial mean arterial pressure, base deficit, Glasgow Coma Scale (GCS) score, activated partial thromboplastin time, platelet count, and fibrinogen level were independent predictors of in-hospital mortality. Initial GCS score, head Abbreviated Injury Scale score of 5, platelet count, and massive transfusion were independent predictors of unfavourable functional outcomes.
Conclusion
This study demonstrates the high mortality rates in patients with combined severe head and abdominal injuries. The findings also identified key prognostic factors, including initial GCS score and platelet count. Early recognition and management of these factors are crucial for improving outcomes in patients who are critically injured patient.
Molecular Detection of Schistosomiasis using Real-Time PCR pre- and post-treatment in some regions of Nigeria
Authors: Muzerengwa Reward , Ndams Iliya Shehu , Sibula Madeline , Mbanga Joshua , Mdul Takafira , Savadye Deckster Tonny 
Published: November 2024

Abstract

Background
Schistosomiasis, a neglected tropical disease, is a major helminth disease in terms of morbidity and mortality that affects more than 200 million people worldwide. This study aimed to use real-time PCR (RT‒PCR) for the detection of Schistosoma sp. DNA in both urine and faeces samples before and after praziquantel (PZQ) treatment, and the results were compared with those of conventional PCR and microscopic detection of schistosome eggs to evaluate treatment efficacy.
Methods
Both urine and stool samples were collected from three hundred and eighty seven (387) study participants aged between 3 and 25 years before and after (3 weeks and 8 weeks) treatment with PZQ in Dumbi communities, Nigeria. On the treatment day, all participants who tested positive after microscopic examination (29) in the community were treated with a single dose of 40 mg/kg PZQ. DNA was isolated from 50 samples (urine) that tested positive using microscopy in preparation for molecular analysis. Microsoft Excel 2016 and SPSS version 20.0 statistical software were used for data analysis.
Results
Utilising diagnostic methods, microscopy detected Schistosoma haematobium eggs in 7.5% (29) of the urine samples collected before treatment, whereas RT‒PCR amplified DNA in 39.8% (154) of the same samples, and no eggs were detected in the stool samples analysed. Among the diagnostic methods for 50 samples that were used for comparative analysis, RT‒PCR had the highest positive detection of 80%, followed by conventional PCR (72%), haematuria (64%) and microscopy (58%). Compared with microscopy, RT‒PCR and conventional PCR both provided lower estimates of cure rates.
Conclusions
The results of this study revealed that RT‒PCR and conventional PCR are significantly more sensitive than microscopy for detecting and evaluating infection incidence, which is an important aspect of epidemiological studies. The RT‒PCR-based detection technique can be especially useful in circumstances of lower intensity or prevalence of infection, a condition for which the parasitological diagnosis shows a well-proven limitation of its sensitivity.
Enhancement or Skill Acquisition? Investigating the Contradiction of Combining Motor and Cognitive Challenges in sport training sessions
Authors: Ghasemian Mohammadreza , Hosseinalizade Maedeh , Fazeli Davoud 
Published: November 2024

Abstract

This study aimed to investigate the effect of combining cognitive challenges with table tennis training on executive functions and forehand skill acquisition. To do so, 36 beginners were randomly divided into three groups of high cognitive load, low cognitive load, and a control group. Participants were asked to perform the forehand task according to a certain practice designed for each group. Then, variables of inhibition, working memory, mental representation, and forehand accuracy were measured. The results showed that both experimental groups performed better than the control group in terms of inhibition; however, only the high cognitive load group had a significant improvement in terms of working memory and the low cognitive load group had a more structured mental representation than the other two groups. Moreover, the two experimental groups with high and low cognitive load performed more accurate forehand test than the control group. Our results show that practice with different cognitive loads can have different effects on improving cognitive functions and skill acquisition. Hence, the improvement of skill acquisition in both groups and the improvement of mental representation only in the group with low cognitive load could indicate that in the group with high cognitive load, attention has moved away from the skill performance procedure due to the working memory involvement during the practice; also, the participants had improved skill performance although no structured knowledge of the skill has been formed in their memory, which can be considered as a characteristic of the implicit learning style.
Dual energy CT-derived quantitative parameters and hematological characteristics predict pathological complete response in neoadjuvant chemoradiotherapy esophageal squamous cell carcinoma patients
Authors: Li Miaomiao , Cui Yongbin , Yan Yuanyuan , Zhao Junfeng , Lin Xinjun , Liu Qianyu , Dong Shushan , Nie Mingming , Huang Yong , Li Baosheng , Yin Yong 
Published: November 2024

Abstract

Purpose
This study aimed to investigate whether dual layer detector dual energy CT (DECT) quantitative parameters and clinical features could predict pathological complete response(pCR) for esophageal squamous cell carcinoma (ESCC) patients who received neoadjuvant chemoradiotherapy (nCRT).
Material and methods
This study retrospective recruited local advanced ESCC patients who underwent nCRT followed by surgical treatment from December 2019 to January 2023. According to pCR status (no visible cancer cells in primary cancer lesion and lymph nodes), patients were categorized into pCR group (N = 25) and non-pCR group (N = 28). DECT quantitative parameters were derived from conventional CT images, different monoenergetic (MonoE) images, virtual non-contrast (VNC) images, Z-effective (Zeff) images, iodine concentration (IC) images and electron density (ED) images. Slope of spectral curve (λHU), normalized iodine concentration (NIC), arterial enhancement fraction (AEF) and extracellular volume (ECV) were calculated. Difference tests and spearman correlation were used to select quantitative parameters for DECT model building. Multivariate logistic analysis was used to build clinical model, DECT model and combined model. The performance for those models were evaluated by sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and the area under the receiver-operator curve (AUC).
Results
A total of 53 patients with locally advanced ESCC were enrolled in this study who received nCRT combined with surgery and underwent DECT examination before treatment. After spearman correlation analysis and multivariate logistic analysis, AEF and ECV showed significant roles between pCR and non-pCR groups. These two quantitative parameters were selected for DECT model. Multivariate logistic analysis revealed that LMR and RBC were also independent predictors in clinical model. The combined model showed the highest sensitivity, specificity, PPV and NPV compared to the clinical and spectral model. The AUC of the combined model is 0.893 (95%CI: 0.802–0.983). Delong’s test revealed the combined model significantly different from clinical model (Z =-2.741, P = 0.006).
Conclusion
Dual-layer DECT derived ECV fraction and AEF are valuable predictor for pCR in ESCC patients after nCRT. The combined DECT quantitative parameters and clinical features model is aimed to provide guidance for those patients’ treatment.