bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phonological awareness emergence in preschool children with autism spectrum disorder
Authors: Dynia Jaclyn M , Bean Allison , Justice Laura M , Kaderavek Joan N 
Published: January 2019

Background and aims Phonological awareness begins to develop during the preschool years and is a primary factor underlying later reading abilities. Previous research has found mixed results on the phonological awareness skills of children with autism spectrum disorders. Therefore, the purpose of the present study is to add to our understanding of phonological awareness in children with autism spectrum disorder by investigating residualized gains in phonological awareness skills over an academic year. Methods A total of 125 preschool (4- to 5-years old) children including 27 children with autism spectrum disorder, 28 children with language impairment, and 70 typically developing children were the focus of the present study. Participants in the current study represent a subset of participants from a larger study titled, Sit Together and Read. Children completed direct assessments in the beginning (fall) and end of school year (spring) on phonological awareness using the Test of Preschool Early Literacy. Results A one-way ANOVA compared the phonological awareness skills tasks (syllable/onset-rime, blending/elision, receptive/expressive) in the fall and the spring for each of the three groups (children with autism spectrum disorder, children with language impairment, children who are typically developing). In the fall and the spring, all of the analyses were found to be statistically significant. A Tukey HSD further indicated that children with autism spectrum disorder had significantly lower scores on all of the tasks at both time points compared to the typically developing peers. Children with autism spectrum disorder seem to make gains in phonological awareness tasks similarly to their typically developing peers for most of the phonological awareness tasks. Results from the final regression models indicated that children with autism spectrum disorder made gains similar to those of their typically developing peers for most phonological awareness tasks and that language skills predicted residualized gain for syllable, elision, and receptive tasks, as well as the total score when controlling for condition, IQ, and group status. Social skills were not a significant predictor for any of the tasks. Conclusions Three main findings emerged: (a) phonological awareness skills seem to be a deficit for children with autism spectrum disorder, (b) in general, autism status does not predict residualized gain in phonological awareness skills, and (c) oral language is a significant predictor of residualized gain in phonological awareness skills. Implication Early childhood educators should focus on providing high-quality instruction on phonological awareness for children with autism spectrum disorder and researchers should focus on investigating the effectiveness of phonological awareness interventions for children with autism spectrum disorder.
A genome-wide association study identifies new loci for factor VII and implicates factor VII in ischemic stroke etiology
Authors: de Vries Paul S. , Sabater-Lleal Maria , Huffman Jennifer E. , Marten Jonathan , Song Ci , Pankratz Nathan , Bartz Traci M. , de Haan Hugoline G. , Delgado Graciela E. , Eicher John D. , Martinez-Perez Angel , Ward-Caviness Cavin K. , Brody Jennifer A. , Chen Ming-Huei , de Maat Moniek P. M. , Frånberg Mattias , Gill Dipender , Kleber Marcus E. , Rivadeneira Fernando , Soria José Manuel , Tang Weihong , Tofler Geoffrey H. , Uitterlinden André G. , van Hylckama Vlieg Astrid , Seshadri Sudha , Boerwinkle Eric , Davies Neil M. , Giese Anne-Katrin , Ikram M. Kamran , Kittner Steven J. , McKnight Barbara , Psaty Bruce M. , Reiner Alex P. , Sargurupremraj Muralidharan , Taylor Kent D. ,   INVENT Consortium,   MEGASTROKE Consortium of the International Stroke Genetics Consortium, Fornage Myriam , Hamsten Anders , März Winfried , Rosendaal Frits R. , Souto Juan Carlos , Dehghan Abbas , Johnson Andrew D. , Morrison Alanna C. , O'Donnell Christopher J. , Smith Nicholas L. 
Published: January 2019

Abstract
Factor VII (FVII) is an important component of the coagulation cascade. Few genetic loci regulating FVII activity and/or levels have been discovered to date. We conducted a meta-analysis of 9 genome-wide association studies of plasma FVII levels (7 FVII activity and 2 FVII antigen) among 27 495 participants of European and African ancestry. Each study performed ancestry-specific association analyses. Inverse variance weighted meta-analysis was performed within each ancestry group and then combined for a trans-ancestry meta-analysis. Our primary analysis included the 7 studies that measured FVII activity, and a secondary analysis included all 9 studies. We provided functional genomic validation for newly identified significant loci by silencing candidate genes in a human liver cell line (HuH7) using small-interfering RNA and then measuring F7 messenger RNA and FVII protein expression. Lastly, we used meta-analysis results to perform Mendelian randomization analysis to estimate the causal effect of FVII activity on coronary artery disease, ischemic stroke (IS), and venous thromboembolism. We identified 2 novel (REEP3 and JAZF1-AS1) and 6 known loci associated with FVII activity, explaining 19.0% of the phenotypic variance. Adding FVII antigen data to the meta-analysis did not result in the discovery of further loci. Silencing REEP3 in HuH7 cells upregulated FVII, whereas silencing JAZF1 downregulated FVII. Mendelian randomization analyses suggest that FVII activity has a positive causal effect on the risk of IS. Variants at REEP3 and JAZF1 contribute to FVII activity by regulating F7 expression levels. FVII activity appears to contribute to the etiology of IS in the general population.
Risk stratification models in human papillomavirus-associated oropharyngeal squamous cell carcinoma: The Nova Scotia distribution
Authors: Forner David , Rigby Matthew H. , Wilke Derek , Taylor S. Mark , Lamond Nathan 
Published: January 2019

Objective The incidence of oropharyngeal squamous cell carcinoma is increasing with a growing proportion of diagnoses associated with human papillomavirus (p16 + OSCC), which generally confers a favorable prognosis. For these reasons, novel risk stratification models specific to the p16 + OSCC population have recently been proposed to guide future research on treatment de-intensification for appropriate patients. This study aimed to quantify patient risk distribution using multiple published risk models and investigate the hypothesis that the local p16 + OSCC population includes a smaller proportion of low-risk patients due to a high prevalence of concurrent tobacco exposure. Methods A retrospective cohort study was performed including patients diagnosed with p16 + OSCC in Nova Scotia between 2011 and 2015. Patient identification was obtained through the CCNS registry and an institutional database. Exclusion criteria included HPV negative status, second primary cases, incomplete data availability, and local recurrence cases. Results Following exclusion, 117 patients met study criteria. The majority had small primary tumors (70.9% ≤ T2) and advanced nodal status on presentation (60.7% ≥ N2b). Most patients had a positive smoking history (62.4%), with 53.0% of patients having a pack-year history greater than 10 pack-years. In four of the five risk stratification models, the majority of the study population fell into the lowest risk category. The risk stratification distribution of our local population was similar to the populations used to validate the published models, with the largest single category difference being 13.3% (range − 12.3 to + 13.3%). Conclusions This is the first study to compare multiple currently published risk stratification models to a local population and address the uncertainty of risk stratification in the Nova Scotian p16 + OSCC population. Despite a high prevalence of concurrent tobacco exposure, the study population was found to be overall low risk, with similar risk compared to model validation populations.
Myc is dispensable for cardiomyocyte development but rescues Mycn-deficient hearts through functional replacement and cell competition
Authors: Muñoz-Martín Noelia , Sierra Rocío , Schimmang Thomas , Villa del Campo Cristina , Torres Miguel 
Published: January 2019

ABSTRACT
Myc is considered an essential transcription factor for heart development, but cardiac defects have only been studied in global Myc loss-of-function models. Here, we eliminated Myc by recombining a Myc floxed allele with the Nkx2.5Cre driver. We observed no anatomical, cellular or functional alterations in either fetuses or adult cardiac Myc-deficient mice. We re-examined Myc expression during development and found no expression in developing cardiomyocytes. In contrast, we confirmed that Mycn is essential for cardiomyocyte proliferation and cardiogenesis. Mosaic Myc overexpression in a Mycn-deficient background shows that Myc can replace Mycn function, recovering heart development. We further show that this recovery involves the elimination of Mycn-deficient cells by cell competition. Our results indicate that Myc is dispensable in cardiomyocytes both during cardiogenesis and for adult heart homeostasis, and that Mycn is exclusively responsible for cardiomyocyte proliferation during heart development. Nonetheless, our results show that Myc can functionally replace Mycn. We also show that cardiomyocytes compete according to their combined Myc and Mycn levels and that cell competition eliminates flawed cardiomyocytes, suggesting its relevance as a quality control mechanism in cardiac development.
The Human Cell Atlas: making ‘cell space’ for disease
Authors: Ponting Chris P. 
Published: January 2019

ABSTRACT
A single change in DNA, RNA, proteins or cellular images can be useful as a biomarker of disease onset or progression. With high-throughput molecular phenotyping of single cells, it is now conceivable that the molecular changes occurring across thousands, or tens of thousands, of individual cells could additionally be considered as a disease biomarker. Transition to a disease state would then be reflected by the shifts in cell numbers and locations across a multidimensional space that is defined by the molecular content of cells. Realising this ambition requires a robust formulation of such a multidimensional ‘cell space’. This is one of the goals of the recently launched Human Cell Atlas project. A second goal is to populate this ‘cell space’ with all cell types in the human body. Here, I consider the potential of the Human Cell Atlas project for improving our description and understanding of the cell-type specificity of disease.
Exact Algorithms for the Vehicle Routing Problem with Time Windows and Combinatorial Auction
Authors: Zhang Zhenzhen , Luo Zhixing , Qin Hu , Lim Andrew 
Published: January 2019

In this paper, we address an interesting variant of the vehicle routing problem with time windows (VRPTW) faced by the China National Petroleum Corporation (CNPC). The CNPC owns a limited number of tanker trucks for delivering the petroleum to oil stations within specific time windows in the regular seasons. However, during the peak seasons, some requests need to be outsourced to external third-party logistics companies. These companies provide several bids, each of which includes the oil stations to be served and the corresponding charges. The CNPC needs to select some bids and design routes for the self-owned trucks, so that all requests are satisfied and the total cost is minimized. To study this problem, we formulate it into an arc-flow model and a set-partitioning model, and propose six families of valid inequalities to strengthen the set-partitioning model. Based on the set-partitioning model, we propose a branch-and-price-and-cut algorithm and a branch-and-bound algorithm to solve the problem exactly. The proposed algorithms are tested on instances generated according to the well-known Solomon’s benchmark instances for the VRPTW and read-world data of the CNPC. The computational experiments demonstrate the effectiveness of the proposed algorithms. The online appendix is available at https://doi.org/10.1287/trsc.2018.0835 .
Adaptive Large Neighborhood Search with a Constant-Time Feasibility Test for the Dial-a-Ride Problem
Authors: Gschwind Timo , Drexl Michael 
Published: January 2019

In the dial-a-ride problem, user-specified transport requests from origin to destination points have to be served by a fleet of homogeneous vehicles. The problem variant we consider aims at finding a set of minimum-cost routes satisfying constraints on vehicle capacity, time windows, maximum route duration, and maximum user ride times. We propose an adaptive large neighborhood search (ALNS) for its solution. The key novelty of the approach is an exact amortized constant-time algorithm for evaluating the feasibility of request insertions in the repair steps of the ALNS. In addition, we use two optional improvement techniques: a local-search-based intraroute improvement of routes of promising solutions using the Balas–Simonetti neighborhood and the solution of a set covering model over a subset of all routes generated during the search. With these techniques, the proposed algorithm outperforms the state-of-the-art methods in terms of solution quality. New best solutions are found for several benchmark instances. The online appendix is available at https://doi.org/10.1287/trsc.2018.0837 .
Malawi and HPV: Sustainable approaches to treatment
Authors: Winter George F , Cubie Heather A 
Published: January 2019

Human papillomavirus (HPV) is an extremely common viral infection, with more than 100 different variations. It can have serious consequeneces, however, including cervical cancer, which is caused by two variations of the disease in particular (Types 16 and 18). Malawi has the second-highest rate of cervical cancer in the world. In 2013, the Scottish Government provided funding to researchers aiming to reduce the burden of the disease in Malawi as part of a sustainable partnership between the two countries. Professor Heather A Cubie led research into a same-day ‘screen and treat’ programme to assess its suitability for use in low-resource countries such as Malawi, and discusses her research.
Surgical Intervention in Upper Lid Haemangioma To Prevent Amblyopia: Two Cases Report
Authors: Sudjatmiko Gentur , Marita Adinda 
Published: January 2019

Non-operative treatment for Hemangioma involving deeper skin structures extending into the mucosa is usually unsuccessful, since usually no involution will develop. Early surgical planning is important to prevent amblyopia and for the child’s self confidence of their cosmetic appearance later in life. We present two cases of superior palpebra hemangioma who underwent early surgical treatment. The first case involves a large hemangioma of the left superior palpebra with severe vision restriction; although later examination was proven that the visual function was still preserved. The second case involves a smaller perioccular lesion. Surgery was performed on both cases. In the larger hemangioma, only partial of tumor was removed by incision at the lid crease and intra-lesion injection of corticosteroids was administered to the remaining tumor.Advantages of surgical procedure in early rapidly growing stage and in very large hemangioma of upper eyelid; firstly, provides a definitive early treatment; secondly, safe because bleeding was manageable, and third prevent occlusion-related amblyopia. Besides these bene!ts surgery promises added esthetic outcome.
Lesson From Other Discipline’s Decision in Managing Giant Haemangioma Of The Hemithorax : Case Report
Authors: Djaprie Shelly M , Sudjatmiko Gentur 
Published: January 2019

The management of the giant and moderate size infantile haemangiomas are challenging problems, especially in health systems with limited resources in developing countries. The aim of presenting this case is to take a lesson from another discipline’s decision in managing haemangioma. The author provide information based on clinical examination and surgical records of the patient with giant hemangioma which was consulted to plastic surgery team. A four month year old boy was consulted by pediatric surgery team with a giant size haemangioma on the right hemithorax.The treatment option for each haemangioma are different based on the case itself. Especially for this case, it seems better to be treated conservatively due to several reason, such as the phase of hemangioma, the location of the mass, the size, the donor morbidity. Early surgical excision of a moderate size infantile haemangioma may be justified especially when there is dificulty of follow-up. This approach will prevent growth deformation, impact on nearby vital organs and psychological problems.
Treatment of Buccal Lymph-hemangioma by Suction and Intralesional Steroid Injection
Authors: Wardhana Aditya , Sudjatmiko Gentur , Tiza Melina 
Published: January 2019

Lymphangioma is a tumor-like lesion of lymphatics that most often located in the head and neck area, usually causing marked cosmetic and functional problems. Challenge continues to find a safe and effective modality of treatment for anatomical and functional purposes. Until now, no satisfactory methods in lymphangioma elimination. A 12 years old boy with lymphangioma of the left buccal was referred to the division of Plastic and Reconstructive Surgery in Cipto Mangunkusumo hospital. Suction and intralesional steroid injection was decided as a treatment in this patient. Pressure garment customized the size was made and applied to maintain and prevent healing in sagging position.Long term result should be evaluated, in this patient since the repair just only for 5 months. At follow up, 5 months post operatively after Suction and intralesional steroid injection, the buccal had improved significantly with normal anatomical and functional result. In our experience, the correction of the buccal lymph-hemangioma with suction and intralesional steroid injection was achieved a satisfactory result.
Revisited Anterolateral Thigh Free Flaps For Reconstructive Microsurgery : One Year Evaluation
Authors: Atmodiwirjo Parintosa , Handayani Siti , Djaprie Shelly Madona 
Published: January 2019

Extensive soft tissue defects present a dif!cult problem to the plastic surgeon as they are usually associated with exposed important structures such as vessels, nerves, tendons, joint cavity or bone. Reconstruction of soft tissue defects have a wide range of therapeutic options. We reconstructed soft tissue defect in many areas using free anterolateral thigh flap (ALTF). From Februari 2009 - 2010, 9 cases of soft tissue defects in the face, neck, leg and foot of various etiologic factors were admitted to the plastic and reconstructive surgery unit, Cipto Mangunkusumo general hospital. Trauma is the commonest cause of soft tissue defects of the lower extremity, followed by tumours. The cruris was the commonest site (4 cases, 44,4%). Flap success rate was 66,67 %. Failure was reported 1 cases in this study due to vein compromise. In our hospital, we are quite familiar with Anterolateral thigh flap (ALTF) even though the case is limited. Anterolateral thigh flap (ALTF) is used for reconstruction of various simple and complex soft tissue defects, for big and small defects with cavity (orbita).
Self Made Nostril Retainer
Authors: Raymond Benni , Sudjatmiko Gentur 
Published: January 2019

The main feature of the unilateral cleft lip nasal deformity is the asymmetry of the nose compared with the non cleft side. Nasal stents or nostril retainers are suggested to be useful in the management of these patients in order to maintain the new morphology and to mold the shape during healing process. To date, there are many types of factory made nostril retainer, but the price is relatively expensive and in contrary, most of our patient came from low-income population. The idea is to provide an easy and practical nostril retainer to obtain, easy to manufacture and easy to apply preoperatively and postoperatively, and it is also cost effective. Primary nose model including the nasal cavity is designed on the basis of non cleft side of patient’s nose. It is made by injecting alginate as negative impression. We use gypsum powder as positive impression material. From that positive impression, we mirror it to create other side of the nostril. Nostril retainer is then made using home-use silicone gel. We postulate that by using self-made nostril retainer which is made using cheap material. All of our patients could afford one, because the use of nostril retainer has been found effective post operatively after cleft primary rhinoplasty, avoiding relapse of the nasal reconstruction and drop of the nasal ala.
Antibacterial Activity of Indonesian Local Honey Against Strains of P. Aeruginosa, S. Aureus and MRSA
Authors: K. P. Ayu Diah , Sundoro Ali , Sudjatmiko Gentur 
Published: January 2019

Background: Honey has been used in wound care since ancient times. Many publications attest antibacterial activity of manuka honey against Pseudomonas aeruginosa, S. aureus, and Methicillin-resistant S. aureus (MRSA). However, antibacterial effect of local honey from Indonesia has never been studied and compared to medical grade honey before. This study aim to compare antibacterial activity of local honey and manuka honey against P. aeruginosa, S. aureus and MRSA.Methods: The honeys were tested for their antimicrobial activities with broth dilution method. Different concentrations of honey were prepared in reaction tubes. Strains of P.aeruginosa, S. aureus, and MRSA were grown in nutrient broth. These strains of bacteria then added to the different concentrations of honey. Minimum Inhibitory Concentrations (MIC) is the lowest concentration of the honey that yielded no growth of bacteria.Results: MIC of local honey for P. aeruginosa is 50%, for S. aureus is 100% and for MRSA is 100%. MIC of manuka honey for P. aeruginosa is 12,5%, for S. aureus is 25%, and for MRSA is 12,5%.Conclusion: Nusantara Manuka have the similar antibacterial activity spectrum against P. aeruginosa, MRSA and S. aureus, but it must be given in greater concentration to exert the same level of antibacterial activity with Manuka honey.
Granulocyte-Macrophage Colony Stimulating Factor and Steroid on Neovascularization and Keratinocyte Proliferation in Wound Healing
Authors: Naim Zainul , Supit Laureen , Sutrisno Eddy , Buchari Frank Bietra 
Published: January 2019

Background: This study aim to evaluate the effect of subcutaneous application of recombinant human granuloctyte-macrophage colony stimulating factor (rhGM-CSF) around wounds and how it in"uences the speed of wound healing. Methods: The study utilizes Mus musculus mice in a controlled laboratory setting. Mice are divided into 3 groups: A (n = 4) receiving rhGM-CSF 10?g/kg, B (n = 4) receiving dexamethasone 10 mg/kg and C (n = 5) receiving placebo as control. Full thickness wound was made, and either rhGM-CSF, dexamethasone, or nothing were given on the wound subcutaneously for 6 days. On day 7, all rats were sacrificed and a 4-mm area from the edge of the wound were subjected for histologic examinations. Pattern of neovascularization and keratinocyte proliferation were analyzed.Results: The data shows a higher rate of neovascularization and keratinocyte proliferation in the rhGMCSF group compared to the steroid and placebo groups (p = 0.001). Not difference in the rate of keratinocyte proliferations (p = 0.085) and neovascularization (p = 0.935) are found between the dexamethasone and control groups.Discussion: Granulocyte macrophage colony stimulating factor (GM-CSF) hastens wound healing in wildtype mice by increasing the rate of keratinocyte proliferation and neovascular formation, while dexamethasone has a tendency to hinder wound healing because it acts as a GM-CSF inhibitor.