bluuebunny/crossref_metadata_2025_split huggingface dataset:
TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn
Authors: Montalbán-Loro Raquel , Lozano-Ureña Anna , Ito Mitsuteru , Krueger Christel , Reik Wolf , Ferguson-Smith Anne C. , Ferrón Sacri R. 
Published: April 2019

AbstractTen-eleven-translocation (TET) proteins catalyze DNA hydroxylation, playing an important role in demethylation of DNA in mammals. Remarkably, although hydroxymethylation levels are high in the mouse brain, the potential role of TET proteins in adult neurogenesis is unknown. We show here that a non-catalytic action of TET3 is essentially required for the maintenance of the neural stem cell (NSC) pool in the adult subventricular zone (SVZ) niche by preventing premature differentiation of NSCs into non-neurogenic astrocytes. This occurs through direct binding of TET3 to the paternal transcribed allele of the imprinted gene Small nuclear ribonucleoprotein-associated polypeptide N (Snrpn), contributing to transcriptional repression of the gene. The study also identifies BMP2 as an effector of the astrocytic terminal differentiation mediated by SNRPN. Our work describes a novel mechanism of control of an imprinted gene in the regulation of adult neurogenesis through an unconventional role of TET3.
Integration of a (–Cu–S–)n plane in a metal–organic framework affords high electrical conductivity
Authors: Pathak Abhishek , Shen Jing-Wen , Usman Muhammad , Wei Ling-Fang , Mendiratta Shruti , Chang Yu-Shin , Sainbileg Batjargal , Ngue Chin-May , Chen Ruei-San , Hayashi Michitoshi , Luo Tzuoo-Tsair , Chen Fu-Rong , Chen Kuei-Hsien , Tseng Tien-Wen , Chen Li-Chyong , Lu Kuang-Lieh 
Published: April 2019

AbstractDesigning highly conducting metal–organic frameworks (MOFs) is currently a subject of great interest for their potential applications in diverse areas encompassing energy storage and generation. Herein, a strategic design in which a metal–sulfur plane is integrated within a MOF to achieve high electrical conductivity, is successfully demonstrated. The MOF {[Cu2(6-Hmna)(6-mn)]·NH4}n (1, 6-Hmna = 6-mercaptonicotinic acid, 6-mn = 6-mercaptonicotinate), consisting of a two dimensional (–Cu–S–)n plane, is synthesized from the reaction of Cu(NO3)2, and 6,6′-dithiodinicotinic acid via the in situ cleavage of an S–S bond under hydrothermal conditions. A single crystal of the MOF is found to have a low activation energy (6 meV), small bandgap (1.34 eV) and a highest electrical conductivity (10.96 S cm−1) among MOFs for single crystal measurements. This approach provides an ideal roadmap for producing highly conductive MOFs with great potential for applications in batteries, thermoelectric, supercapacitors and related areas.
Inner-shell clock transition in atomic thulium with a small blackbody radiation shift
Authors: Golovizin A. , Fedorova E. , Tregubov D. , Sukachev D. , Khabarova K. , Sorokin V. , Kolachevsky N. 
Published: April 2019

AbstractOne of the key systematic effects limiting the performance of state-of-the-art optical clocks is the blackbody radiation (BBR) shift. Here, we demonstrate unusually low sensitivity of a 1.14 μm inner-shell clock transition in neutral Tm atoms to BBR. By direct polarizability measurements, we infer a differential polarizability of the clock levels of −0.063(30) atomic units corresponding to a fractional frequency BBR shift of only 2.3(1.1) × 10−18 at room temperature. This amount is several orders of magnitude smaller than that of the best optical clocks using neutral atoms (Sr, Yb, Hg) and is competitive with that of ion optical clocks (Al+, Lu+). Our results allow the development of lanthanide-based optical clocks with a relative uncertainty at the 10−17 level.
Plasmonic IQ modulators with attojoule per bit electrical energy consumption
Authors: Heni Wolfgang , Fedoryshyn Yuriy , Baeuerle Benedikt , Josten Arne , Hoessbacher Claudia B. , Messner Andreas , Haffner Christian , Watanabe Tatsuhiko , Salamin Yannick , Koch Ueli , Elder Delwin L. , Dalton Larry R. , Leuthold Juerg 
Published: April 2019

AbstractCoherent optical communications provides the largest data transmission capacity with the highest spectral efficiency and therefore has a remarkable potential to satisfy today’s ever-growing bandwidth demands. It relies on so-called in-phase/quadrature (IQ) electro-optic modulators that encode information on both the amplitude and the phase of light. Ideally, such IQ modulators should offer energy-efficient operation and a most compact footprint, which would allow high-density integration and high spatial parallelism. Here, we present compact IQ modulators with an active section occupying a footprint of 4 × 25 µm × 3 µm, fabricated on the silicon platform and operated with sub-1-V driving electronics. The devices exhibit low electrical energy consumptions of only 0.07 fJ bit−1at 50 Gbit s−1, 0.3 fJ bit−1at 200 Gbit s−1, and 2 fJ bit−1at 400 Gbit s−1. Such IQ modulators may pave the way for application of IQ modulators in long-haul and short-haul communications alike.
Immunoregulation of macrophages by dynamic ligand presentation via ligand–cation coordination
Authors: Kang Heemin , Yang Boguang , Zhang Kunyu , Pan Qi , Yuan Weihao , Li Gang , Bian Liming 
Published: April 2019

AbstractMacrophages regulate host responses to implants through their dynamic adhesion, release, and activation. Herein, we employ bisphosphonate (BP)-coated gold nanoparticle template (BNP) to direct the swift and convertible formation of Mg2+-functional Mg2+-BP nanoparticle (NP) on the BP-AuNP surface via reversible Mg2+-BP coordination, thus producing (Mg2+-BP)-Au dimer (MgBNP). Ethylenediaminetetraacetic acid-based Mg2+ chelation facilitates the dissolution of Mg2+-BP NP, thus enabling the reversion of the MgBNP to the BNP. This convertible nanoassembly incorporating cell-adhesive Mg2+ moieties directs reversible attachment and detachment of macrophages by BP and EDTA, without physical scraping or trypsin that could damage cells. The swift formation of RGD ligand- and Mg2+-bifunctional RGD-Mg2+-BP NP that yields (RGD-Mg2+-BP)-Au dimer (RGDBNP) further stimulates the adhesion and pro-regenerative M2-type polarization of macrophages, both in vitro and in vivo, including rho-associated protein kinase. This swift and non-toxic dimer formation can include diverse bio-functional moieties to regulate host responses to implants.
Microwave photons emitted by fractionally charged quasiparticles
Authors: Bisognin R. , Bartolomei H. , Kumar M. , Safi I. , Berroir J.-M. , Bocquillon E. , Plaçais B. , Cavanna A. , Gennser U. , Jin Y. , Fève G. 
Published: April 2019

AbstractStrongly correlated low-dimensional systems can host exotic elementary excitations carrying a fractional charge q and potentially obeying anyonic statistics. In the fractional quantum Hall effect, their fractional charge has been successfully determined owing to low frequency shot noise measurements. However, a universal method for sensing them unambiguously and unraveling their intricate dynamics was still lacking. Here, we demonstrate that this can be achieved by measuring the microwave photons emitted by such excitations when they are transferred through a potential barrier biased with a dc voltage Vdc. We observe that only photons at frequencies f below qVdc/h are emitted. This threshold provides a direct and unambiguous determination of the charge q, and a signature of exclusion statistics. Derived initially within the Luttinger model, this feature is also predicted by universal non-equilibrium fluctuation relations which agree fully with our measurements. Our work paves the way for further exploration of anyonic statistics using microwave measurements.
Localized corrosion of low-carbon steel at the nanoscale
Authors: Hayden Steven C. , Chisholm Claire , Grudt Rachael O. , Aguiar Jeffery A. , Mook William M. , Kotula Paul G. , Pilyugina Tatiana S. , Bufford Daniel C. , Hattar Khalid , Kucharski Timothy J. , Taie Ihsan M. , Ostraat Michele L. , Jungjohann Katherine L. 
Published: April 2019

AbstractMitigating corrosion remains a daunting challenge due to localized, nanoscale corrosion events that are poorly understood but are known to cause unpredictable variations in material longevity. Here, the most recent advances in liquid-cell transmission electron microscopy were employed to capture the advent of localized aqueous corrosion in carbon steel at the nanoscale and in real time. Localized corrosion initiated at a triple junction formed by a solitary cementite grain and two ferrite grains and then continued at the electrochemically-active boundary between these two phases. With this analysis, we identified facetted pitting at the phase boundary, uniform corrosion rates from the steel surface, and data that suggest that a re-initiating galvanic corrosion mechanism is possible in this environment. These observations represent an important step toward atomically defining nanoscale corrosion mechanisms, enabling the informed development of next-generation inhibition technologies and the improvement of corrosion predictive models.
Effect of radiochemotherapy on the cognitive function and diffusion tensor and perfusion weighted imaging for high-grade gliomas: A prospective study
Authors: Bian Yiying , Meng Li , Peng Jianghua , Li Junfeng , Wei Rui , Huo Lei , Yang Huan , Wang Ying , Fu Jun , Shen Liangfang , Hong Jidong 
Published: April 2019

AbstractThis study aimed to explore the effects of radiochemotherapy on the neurocognitive function of patients with high-grade gliomas (HGG). The mini-mental state examination (MMSE), Montreal Cognitive Assessment (MoCA), event-related potential P300 (ERP-P300), and specific MRI parameters were compared, and the associations between specific MRI parameters and different doses of radiation were determined for before and up to 12 months after radiotherapy. There were no significant differences in MMSE, MoCA, or ERP-P300 before and after radiotherapy. Compared with pre-radiochemotherapy, fractional anisotropy (FA) in the contralateral hippocampus decreased at 6 and 9 months after radiotherapy. FA in the ipsilateral hippocampus before radiochemotherapy decreased compared with 6 months after radiotherapy. Compared to the end of radiotherapy, as well as 3- and 6-months post-radiotherapy, the regional cerebral blood volume (rCBV) in the genu of the corpus was significantly lower at 12 months post-radiotherapy. Some MRI parameters in different regions of the brain were negatively correlated with the mean and maximum dose. There was no significant effect of radiochemotherapy on the neurocognitive functioning of patients with HGGs found before radiochemotherapy until 12 months after radiotherapy. The radiation-induced FA decrease in the bilateral hippocampus preceded cognitive dysfunction, and DTI of the hippocampus may provide a useful biomarker for predicting radiation-induced neurocognitive impairment in patients with HGGs.
Prognostic models based on imaging findings in glioblastoma: Human versus Machine
Authors: Molina-García David , Vera-Ramírez Luis , Pérez-Beteta Julián , Arana Estanislao , Pérez-García Víctor M. 
Published: April 2019

AbstractMany studies have built machine-learning (ML)-based prognostic models for glioblastoma (GBM) based on radiological features. We wished to compare the predictive performance of these methods to human knowledge-based approaches. 404 GBM patients were included (311 discovery and 93 validation). 16 morphological and 28 textural descriptors were obtained from pretreatment volumetric postcontrast T1-weighted magnetic resonance images. Different prognostic ML methods were developed. An optimized linear prognostic model (OLPM) was also built using the four significant non-correlated parameters with individual prognosis value. OLPM achieved high prognostic value (validation c-index = 0.817) and outperformed ML models based on either the same parameter set or on the full set of 44 attributes considered. Neural networks with cross-validation-optimized attribute selection achieved comparable results (validation c-index = 0.825). ML models using only the four outstanding parameters obtained better results than their counterparts based on all the attributes, which presented overfitting. In conclusion, OLPM and ML methods studied here provided the most accurate survival predictors for glioblastoma to date, due to a combination of the strength of the methodology, the quality and volume of the data used and the careful attribute selection. The ML methods studied suffered overfitting and lost prognostic value when the number of parameters was increased.
Activation of immune responses against the basement membrane component collagen type IV does not affect the development of atherosclerosis in ApoE-deficient mice
Authors: Vallejo J. , Dunér P. , To F. , Engelbertsen D. , Gonçalves I. , Nilsson J. , Bengtsson E. 
Published: April 2019

AbstractOxidation of low-density lipoprotein (LDL) in the arterial extracellular matrix results in malondialdehyde (MDA)-modifications of surrounding matrix proteins. We have recently demonstrated an association between high levels of autoantibodies against MDA-modified collagen type IV and risk for development of myocardial infarction. Collagen type IV is an important component of the endothelial basement membrane and influences smooth muscle cell function. We hypothesized that immune responses against collagen type IV could contribute to vascular injury affecting the development of atherosclerosis. To investigate this possibility, we induced an antibody-response against collagen type IV in apolipoprotein E (Apo E)-deficient mice. Female ApoE−/− mice on C57BL/6 background were immunized with α1α2 type IV collagen chain peptides linked to the immune-enhancer PADRE, PADRE alone or PBS at 12 weeks of age with three subsequent booster injections before the mice were killed at 23 weeks of age. Immunization of PADRE alone induced autoantibodies against PADRE, increased IL-4 secretion from splenocytes and reduced SMC content in the subvalvular plaques. Immunization with peptides of α1α2 type IV collagen chains induced a strong IgG1antibody response against collagen type IV peptides without affecting the distribution of T cell populations, plasma cytokine or lipid levels. There were no differences in atherosclerotic plaque development between collagen α1α2(IV)-PADRE immunized mice and control mice. Our findings demonstrate that the presence of antibodies against the basement membrane component collagen type IV does not affect atherosclerosis development in ApoE−/− mice. This suggests that the association between autoantibodies against collagen type IV and risk for myocardial infarction found in humans does not reflect a pathogenic role of these autoantibodies.
Single trial prestimulus oscillations predict perception of the sound-induced flash illusion
Authors: Kaiser Mathis , Senkowski Daniel , Busch Niko A. , Balz Johanna , Keil Julian 
Published: April 2019

AbstractIn the sound-induced flash illusion, auditory input affects the perception of visual stimuli with a large inter- and intraindividual variability. Crossmodal influence in this illusion has been shown to be associated with activity in visual and temporal areas. In this electroencephalography study, we investigated the relationship between oscillatory brain activity prior to stimulus presentation and subsequent perception of the illusion on the level of single trials. Using logistic regression, we modeled the perceptual outcome dependent on oscillatory power. We found that 25 Hz to 41 Hz activity over occipital electrodes from 0.17 s to 0.05 s prior to stimulus onset predicted the perception of the illusion. A t-test of power values, averaged over the significant cluster, between illusion and no-illusion trials showed higher power in illusion trials, corroborating the modeling result. We conclude that the observed power modulation predisposes the integration of audiovisual signals, providing further evidence for the governing role of prestimulus brain oscillations in multisensory perception.
Characterization of donor and recipient CD8+ tissue-resident memory T cells in transplant nephrectomies
Authors: de Leur Kitty , Dieterich Marjolein , Hesselink Dennis A. , Corneth Odilia B. J. , Dor Frank J. M. F. , de Graav Gretchen N. , Peeters Annemiek M. A. , Mulder Arend , Kimenai Hendrikus J. A. N. , Claas Frans H. J. , Clahsen-van Groningen Marian C. , van der Laan Luc J. W. , Hendriks Rudi W. , Baan Carla C. 
Published: April 2019

AbstractTissue-resident memory T (TRM) cells are characterized by their surface expression of CD69 and can be subdivided in CD103+ and CD103− TRM cells. The origin and functional characteristics of TRM cells in the renal allograft are largely unknown. To determine these features we studied TRM cells in transplant nephrectomies. TRM cells with a CD103+ and CD103− phenotype were present in all samples (n = 13) and were mainly CD8+ T cells. Of note, donor-derived TRM cells were only detectable in renal allografts that failed in the first month after transplantation. Grafts, which failed later, mainly contained recipient derived TRM cells. The gene expression profiles of the recipient derived CD8+ TRM cells were studied in more detail and showed a previously described signature of tissue residence within both CD103+ and CD103− TRM cells. All CD8+ TRM cells had strong effector abilities through the production of IFNγ and TNFα, and harboured high levels of intracellular granzyme B and low levels of perforin. In conclusion, our results demonstrate that donor and recipient TRM cells reside in the rejected renal allograft. Over time, the donor-derived TRM cells are replaced by recipient TRM cells which have features that enables these cells to aggressively respond to the allograft.
Sperm physiology varies according to ultradian and infradian rhythms
Authors: Moreno-Irusta Ayelén , Kembro Jackelyn M. , Domínguez Esteban M. , Matamoros-Volante Arturo , Gallea Maria N. , Molina Rosa , Guidobaldi Hector A. , Treviño Claudia L. , Figueras Maria J. , Babini Ana , Paina Nelso A. , Mercado Carlos A. N. , Giojalas Laura C. 
Published: April 2019

AbstractThe spermatozoon must be physiologically prepared to fertilize the egg, process called capacitation. Human sperm samples are heterogeneous in their ability to capacitate themselves, which leads to variability between samples from the same or different donors, and even along the seasons. Here we studied sperm variation in the capacitation state according to the ability of capacitated spermatozoa to acrosome react upon stimulation (% ARi) and to be recruited by chemotaxis (% Chex). Both indirect indicators of sperm capacitation increased along the incubation time with fluctuations. Those capacitated sperm recruited by chemotaxis showed an ultradian rhythm with a cycle every 2 h, which might be influenced by unknown intrinsic sperm factors. Two infradian rhythms of 12 months for the % ARi and of 6 months for % Chex were observed, which are associated with the joint action of temperature and photoperiod. Thus, to avoid false negative results, human sperm samples are recommended to be incubated for a long period (e.g. 18 h) preferably in spring time. This innovative point of view would lead to better comprehend human reproductive biology and to think experimental designs in the light of sperm cyclicity or to improve sperm aptitude for clinical purposes.
An intense, few-cycle source in the long-wave infrared
Authors: Wilson Derrek J. , Summers Adam M. , Zigo Stefan , Davis Brandin , Robatjazi Seyyed-Javad , Powell Jeffery A. , Rolles Daniel , Rudenko Artem , Trallero-Herrero Carlos A. 
Published: April 2019

AbstractFor the last several decades, the wavelength range accessible for strong-field, few-cycle studies has remained limited to the visible, near infrared and mid-wave infrared regimes. In particular, sources in the long-wave infrared have been lacking. We report the development of a 1 kHz, few-cycle laser source with up to a 9 μm central wavelength and gigawatt peak powers. When focused, this source can ionize gas targets, which we demonstrate here through the ionization of atomic xenon at wavelengths ranging from 5 μm to 9 μm. This opens up new opportunities for fundamental atomic and molecular physics, enabling experimental tests of strong-field ionization theories in the extreme long-wavelength, few-cycle limit and the direct excitation of vibrational transitions in organic molecules.
Assembly of Human Stem Cell-Derived Cortical Spheroids and Vascular Spheroids to Model 3-D Brain-like Tissues
Authors: Song Liqing , Yuan Xuegang , Jones Zachary , Griffin Kyle , Zhou Yi , Ma Teng , Li Yan 
Published: April 2019

AbstractHuman cerebral organoids derived from induced pluripotent stem cells (iPSCs) provide novel tools for recapitulating the cytoarchitecture of human brain and for studying biological mechanisms of neurological disorders. However, the heterotypic interactions of neurovascular units, composed of neurons, pericytes, astrocytes, and brain microvascular endothelial cells, in brain-like tissues are less investigated. The objective of this study is to investigate the impacts of neural spheroids and vascular spheroids interactions on the regional brain-like tissue patterning in cortical spheroids derived from human iPSCs. Hybrid neurovascular spheroids were constructed by fusion of human iPSC-derived cortical neural progenitor cell (iNPC) spheroids, endothelial cell (iEC) spheroids, and the supporting human mesenchymal stem cells (MSCs). Single hybrid spheroids were constructed at different iNPC: iEC: MSC ratios of 4:2:0, 3:2:1 2:2:2, and 1:2:3 in low-attachment 96-well plates. The incorporation of MSCs upregulated the secretion levels of cytokines VEGF-A, PGE2, and TGF-β1 in hybrid spheroid system. In addition, tri-cultured spheroids had high levels of TBR1 (deep cortical layer VI) and Nkx2.1 (ventral cells), and matrix remodeling genes, MMP2 and MMP3, as well as Notch-1, indicating the crucial role of matrix remodeling and cell-cell communications on cortical spheroid and organoid patterning. Moreover, tri-culture system elevated blood-brain barrier gene expression (e.g., GLUT-1), CD31, and tight junction protein ZO1 expression. Treatment with AMD3100, a CXCR4 antagonist, showed the immobilization of MSCs during spheroid fusion, indicating a CXCR4-dependent manner of hMSC migration and homing. This forebrain-like model has potential applications in understanding heterotypic cell-cell interactions and novel drug screening in diseased human brain.