bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design, Synthesis and In Vitro Anti-Cancer Evaluation of Novel Derivatives of 2-(2-Methyl-1,5-diaryl-1H-pyrrol-3-yl)-2-oxo-N-(pyridin-3- yl)acetamide
Authors: Moghadam Ebrahim S. , Saravani Farhad , Hamel Ernest , Shahsavari Zahra , Alipour Mohsen , Hosseinkhani Saman , Ostad Seyednasser , Amini Mohsen 
Published: April 2019

Objective:
Several anti-tubulin agents were introduced for the cancer treatment so far.
Despite successes in the treatment of cancer, these agents cause toxic side effects, including peripheral
neuropathy. Comparing anti-tubulin agents, indibulin seemed to cause minimal peripheral
neuropathy, but its poor aqueous solubility and other potential clinical problems have led to its remaining
in a preclinical stage.

Methods:
Herein, indibulin analogues were synthesized and evaluated for their in vitro anti-cancer
activity using MTT assay (on the MCF-7, T47-D, MDA-MB231 and NIH-3T3 cell lines), annexin
V/PI staining assay, cell cycle analysis, anti-tubulin assay and caspase 3/7 activation assay.

Results:
One of the compounds, 4a, showed good anti-proliferative activity against MCF-7 cells
(IC50: 7.5 μM) and low toxicity on a normal cell line (IC50 > 100 μM). All of the tested compounds
showed lower cytotoxicity on normal cell line in comparison to reference compound, indibulin. In
the annexin V/PI staining assay, induction of apoptosis in the MCF-7 cell line was observed. Cell
cycle analysis illustrated an increasing proportion of cells in the sub-G-1 phase, consistent with an
increasing proportion of apoptotic cells. No increase in G2/M cells was observed, consistent with
the absence of anti-tubulin activity. A caspase 3/7 assay protocol showed that apoptosis induction
by more potent compounds was due to activation of caspase 3.

Conclusion:
Newly synthesized compounds exerted acceptable anticancer activity and further investigation
of current scaffold would be beneficial.
ESDA: An Improved Approach to Accurately Identify Human snoRNAs for Precision Cancer Therapy
Authors: Dong Yan-mei , Bi Jia-hao , He Qi-en , Song Kai 
Published: April 2019

Background:
SnoRNAs (Small nucleolar RNAs) are small RNA molecules with approximately
60-300 nucleotides in sequence length. They have been proved to play important roles
in cancer occurrence and progression. It is of great clinical importance to identify new snoRNAs as
fast and accurately as possible.

Objective:
A novel algorithm, ESDA (Elastically Sparse Partial Least Squares Discriminant Analysis),
was proposed to improve the speed and the performance of recognizing snoRNAs from other
RNAs in human genomes.

Methods:
In ESDA algorithm, to optimize the extracted information, kernel features were selected
from the variables extracted from both primary sequences and secondary structures. Then they
were used by SPLSDA (sparse partial least squares discriminant analysis) algorithm as input variables
for the final classification model training to distinguish snoRNA sequences from other Human
RNAs. Due to the fact that no prior biological knowledge is request to optimize the classification
model, ESDA is a very practical method especially for completely new sequences.

Results:
89 H/ACA snoRNAs and 269 C/D snoRNAs of human were used as positive samples and
3403 non-snoRNAs as negative samples to test the identification performance of the proposed
ESDA. For the H/ACA snoRNAs identification, the sensitivity and specificity were respectively as
high as 99.6% and 98.8%. For C/D snoRNAs, they were respectively 96.1% and 98.3%. Furthermore,
we compared ESDA with other widely used algorithms and classifiers: SnoReport, RF
(Random Forest), DWD (Distance Weighted Discrimination) and SVM (Support Vector Machine).
The highest improvement of accuracy obtained by ESDA was 25.1%.

Conclusion:
Strongly proved the superiority performance of ESDA and make it promising for
identifying SnoRNAs for further development of the precision medicine for cancers.
Diseño de evidencias de aprendizaje con base en los aprendizajes clave esperados, en el contexto de la educación virtual
Authors: Reyes López Octavio 
Published: April 2019

<p>Esta investigación tuvo su origen en el diseño de un curso de bachillerato virtual que se sustenta en el Modelo por Competencias en la Educación. Al respecto, se propuso contestar la siguiente pregunta: ¿Cómo diseñar las Evidencias de Aprendizaje (EA) para lograr los <em>aprendizajes clave esperados </em>de los estudiantes en el contexto de la educación virtual? </p><p>Para dar respuesta a esta pregunta se utilizó el esquema EMFIS V-4.0, de donde se generó un constructo teórico, a través de un planteamiento investigativo de análisis heurístico, hermenéutico y fenomenológico, apoyado en la teoría fundamentada. </p><p>El modelo obtenido está representado por 3 dimensiones: los <em>elementos </em>del diseño instruccional, los <em>considerandos </em>para su construcción y el cumplimiento de la <em>representatividad </em>requerida. Del análisis desarrollado, emergieron 13 categorías. </p><p>En las conclusiones se afirma que las Evidencias de Aprendizaje deben ser actividades integradoras y pertinentes, es decir, subordinadas a las competencias, vinculadas a los aprendizajes clave esperados y relacionadas a su contexto social, además de ser tareas retadoras e interesantes</p>
Preferred Learning Styles of Nurses in In-Service Training Courses in Tabriz University of Medical Sciences
Authors: Safaeifard Neda , Areshtanab Hossein Namdar , Roshangar Fariborz , Ebrahim Hossein , Moonaghi Hossein Karimi , Janani Raheleh 
Published: April 2019

Summary
Generally, progress, productivity and success of any organization depends on the skills and knowledge of their manpower. Thus, better and more accurate training programs in organizations will lead to their growth and efficiency will be eventually achieved. Due to the many advances in the field of medicine, nurses are the backbone of activities in organizations of medical sciences and patient’s affairs. For this purpose, in-service training courses for employees are the most important courses in nursing. This study was conducted at the University of Medical Sciences (Tabriz-Iran) aiming to determine the preferred learning styles of nurses in in-service training courses. In this cross-sectional study, all nurses working in medical and educational centers in a university in the North West of Iran were randomly selected. To collect data, a two-part questionnaire of Kolb’s demographic and social information was used. Data was analyzed by using descriptive and analytical statistics SPSS version 17 software. A total of 470 nurses with an average age of 36.46 ± 5.77 were studied. There was a significant correlation between preferred learning styles of nurses with nursing position, employment status, and income level. There was no a significant statistical relationship between the preferred learning style of nurses with age, work experience and experience in the center. The present study shows that the highest percentage of Kolb’s learning style is related to the preferential converging learning style (57.8%). This study aimed to determine the preferred learning styles of nurse’s in-service training courses in Tabriz University of Medical Sciences. The results of the study showed that converging and assimilating styles were the preferred learning ones among the majority of nurses; these styles are effective and interpreted according to their profession requiring a lot of information and knowledge. Due to the dominance of converging learning style among nurses, it is recommended to use appropriate teaching methods tailored to the style including the use of diagrams, presentations, lectures and self-learning with enjoyable materials.
Physiological role of Prion Protein in Copper homeostasis and angiogenic mechanisms of endothelial cells
Authors: De Riccardis Lidia , Rizzo Francesca , Urso Emanuela , Garzarelli Valeria , Intini Vincenza , Greco Marco , Maffia Maria Chiara , Danieli Antonio , Maffia Michele 
Published: April 2019

Abstract
The Prion Protein (PrP) is mostly known for its role in prion diseases, where its misfolding and aggregation can cause fatal neurodegenerative conditions such as the bovine spongiform encephalopathy and human Creutzfeldt–Jakob disease. Physiologically, PrP is involved in several processes including adhesion, proliferation, differentiation and angiogenesis, but the molecular mechanisms behind its role remain unclear. PrP, due to its well-described structure, is known to be able to regulate copper homeostasis; however, copper dyshomeostasis can lead to developmental defects. We investigated PrP-dependent regulation of copper homeostasis in human endothelial cells (HUVEC) using an RNA-interference protocol. PrP knockdown did not influence cell viability in silenced HUVEC (PrPKD) compared to control cells, but significantly increased PrPKD HUVEC cells sensitivity to cytotoxic copper concentrations. A reduction of PrPKD cells reductase activity and copper ions transport capacity was observed. Furthermore, PrPKD-derived spheroids exhibited altered morphogenesis and their derived cells showed a decreased vitality 24 and 48 hours after seeding. PrPKD spheroid-derived cells also showed disrupted tubulogenesis in terms of decreased coverage area, tubule length and total nodes number on matrigel, preserving unaltered VEGF receptors expression levels. Our results highlight PrP physiological role in cellular copper homeostasis and in the angiogenesis of endothelial cells.
Application of whey retentate as complex nitrogen source for growth of the polyhydroxyalkanoate producer Hydrogenophaga pseudoflava strain DSM1023
Authors: Koller Martin , Hesse Paula , Braunegg Gerhart 
Published: April 2019

Abstract
Polyhydroxyalkanoates, microbial polyesters produced in vivo starting from renewable resources, are considered the future materials of choice to compete recalcitrant petro-chemical plastic on the polymer market. In order to make polyhydroxyalkanoates market-fit, (techno)economics of their production need to be improved. Among the multifarious factors affecting costs of polyhydroxyalkanoate production, increased volumetric productivity is of utmost importance. Improving microbial growth kinetics and increasing cell density are strategies leading to a high concentration of catalytically active biomass within a short time; after changing cultivation conditions, these cells can accumulate polyhydroxyalkanoates as intracellular products. The resulting increase of volumetric productivity for polyhydroxyalkanoates can be realized by supplying complex nitrogen sources to growing microbial cultures. In the present study, the impact of different expensive and inexpensive complex nitrogen sources, in particular whey retentate, on the growth and specific growth rates of Hydrogenophaga pseudoflava was tested.
Based on a detailed kinetic process analysis, the study demonstrates that especially whole (not hydrolyzed) whey retentate, an amply available surplus material from dairy industry, displays positive effects on cultivations of H. pseudoflava in defined media (increase of concentration of catalytically active biomass after 26.25 h of cultivation by about 50%, increase of specific growth rate μ from 0.28 to 0.41 1/h during exponential growth), while inhibiting effects (inhibition constant K i
= 6.1 g/L) of acidically hydrolyzed whey retentate need to be overcome. Considering the huge amounts of surplus whey accruing especially in Europe, the combined utilization of whey permeate (carbon source) and whey retentate (complex nitrogen source) for biopolyester production can be considered a viable bioeconomic strategy for the next future.
Influence of personal variables on entrepreneurial intention: A comparative study between Poland and Spain
Authors: Martínez González José Alberto , Kobylińska Urszula 
Published: April 2019

Abstract
This article analyses the influence of personal variables on entrepreneurial intentions of students from Poland and Spain. The article presents an integrated structural model that has been developed from a set of student perceptions from both countries. A small number of variables included in the model allows explaining and managing the formation of the intention in the context of higher education. This study provides answers to the following questions: What role do personal variables play as motivation in the formation of entrepreneurship in the case of young people? Is the motivation stronger than self-efficacy? What are the differences in the perceptions and ratings of students in Poland and Spain? How can these variables be enhanced? This work used a causal quantitative methodology based on structural equations (PLS) and the Smart PLS-3.0 program. The PLS model was chosen for its advantages in the study of human behaviour and its optimal predictive potential, and because it allows the use of reflective indicators. In the causal model generated with a sample of 721 respondents from Poland and Spain, it was found that personal values initiated the chain of effects that influenced the attitude and, through it, successively resulted in motivation, self-efficacy and entrepreneurial intentions. Therefore, the subjective variables (values and attitudes) have a positive and significant influence on the action variables (motivation and self-efficacy), and these affect entrepreneurial intentions. The absence of significant regional differences in the responses to the items and the causal relationships of the model suggests the possibility of developing integrated and homogeneous programmes for the entire segment, thereby achieving synergies. The results suppose a theoretical and practical contribution to the promotion of entrepreneur intentions of university students inside and outside the educational context, suggesting a possible effect of personal variables on entrepreneurial intentions.
The Issue of Leadership Styles in the Military Organization
Authors: Stănciulescu Robert , Beldiman Elvira 
Published: April 2019

Abstract
Enormously studied by specialists, the leadership of organizations is a primordial issue because the leader’s style conditions the efficiency of any social organization. This is one of the reasons why the current scientific concerns focus on studying the ways of improving leadership and the choice of leaders who have the skills and capabilities to successfully perform such functions. This paper deals with the characteristics of the military organization and the complex environment in which it operates, imposing major demands on the military leaders, who must develop certain skills and abilities in order to cope with the wide range of challenges they are confronted with on the modern battlefield. It also points to the features of charismatic leaders, drawing the attention to the relationship between subordinates and leaders, a relationship that is based on an emotional, spiritual, even non-rational bond between leaders and followers.
Using Trucksim for a Virtual Longitudinal Slope Test Procedure
Authors: Roşca Petru , Olaru Gheorghe , Badea Dorel 
Published: April 2019

Abstract
This paper presents a methodology to validate a virtual test procedure for vehicle gradeability performance. It is a test dedicated to both the civilian and military off-road vehicles, an 8 x 8 Armoured Personnel Carrier being selected for the purpose of the paper. TruckSim is the software used to implement the vehicle math model and the test procedure. The simulation results are compared with the experimental results for a 60 % gradient. The vehicle speed and the engine speed graphics, the statistical analyze, and the comparison between the simulation test animation and the experimental test movie are the methods applied to validate the virtual longitudinal slope test procedure.
The assessment of primary teeth condition in 6 year-old children in Podgorica municipality
Authors: Đuričković Mirjana , Ivanović Mirjana , Popović Zorica 
Published: April 2019

Summary
Introduction The most frequent oral disease in children is tooth decay. The aim of this study was to determine the health status of primary teeth in 6 year-old children.
Material and method The study included 203 children of both genders living in the territory of Podgorica municipality. Only children whose parents gave consent were included. The parameters used for assessing oral health condition were: number of decayed, missing, and filled teeth due to caries (dmft) and Significant Caries Index (SiC). One dentist clinically examined all respondents in accordance with methodology and criteria of the World Health Organization (WHO).
Results The average value of dmft in 6-year-olds in Podgorica was 4.9. On average, 80.3% of examined children had dental decay. The SiC Index was 8.3. Among examined children, 12.3% had at least one tooth with fissure sealant. In dmft structure dominated untreated decay (92.6%).
Conclusion Results showed high prevalence of primary teeth decay in 6 years old children, indicating the absence of preventive measures and programs in Montenegro.
Immediate and Short-term Effects of Thoracic Spine Manipulation in Patients With Cervical Radiculopathy: A Randomized Controlled Trial
Authors: Young Ian A. , Pozzi Federico , Dunning James , Linkonis Richard , Michener Lori A. 
Published: April 2019

Background Thoracic spine thrust manipulation has been shown to improve patient-rated outcomes for individuals with neck pain. However, there is limited evidence of its effectiveness in patients with cervical radiculopathy. Objectives To compare the immediate and short-term effects of thoracic manipulation to those of a sham thoracic manipulation in patients with cervical radiculopathy. Methods In this multicenter randomized controlled trial, participants with cervical radiculopathy were randomized to receive either manipulation (n = 22) or sham manipulation (n = 21) of the thoracic spine. Outcomes were measured at baseline, immediately after treatment, and at a follow-up 48 to 72 hours after manipulation. A repeated-measures analysis of variance was used to analyze neck and upper extremity pain (numeric pain-rating scale), disability (Neck Disability Index), cervical range of motion (ROM), and endurance (deep neck flexor endurance test). The chi-square test was used to analyze changes in neck and upper extremity pain, centralization of symptoms, and beliefs about receiving the active manipulation treatment using a global rating of change scale. Results Neck and upper extremity pain, cervical ROM, disability, and deep neck flexor endurance all showed significant interactions between group and time (P<.01). Immediately after treatment and at the 48-to-72-hour follow-up, the manipulation group had lower neck pain (P<.01), better cervical ROM (P<.01), lower disability (P<.01), and better deep neck flexor endurance (P = .02) compared to the sham manipulation group. The manipulation group had moderate to large effect-size changes over time. No between-group differences for upper extremity pain were found immediately following the intervention (P = .34) and at 48 to 72 hours after the intervention (P = .18). At 48 to 72 hours after treatment, a greater proportion of participants in the manipulation group reported improvement (global rating of change scale score of 4 or greater) in neck and upper extremity symptoms (P<.01), centralization of symptoms (P<.01), and beliefs about receiving an active manipulation (P = .01) compared to the sham manipulation group. Conclusion One session of thoracic manipulation resulted in improvements in pain, disability, cervical ROM, and deep neck flexor endurance in patients with cervical radiculopathy. Patients treated with manipulation were more likely to report at least moderate change in their neck and upper extremity symptoms up to 48 to 72 hours following treatment. Level of Evidence Therapy, level 2. J Orthop Sports Phys Ther 2019;49(5):299–309. doi:10.2519/jospt.2019.8150
A Systematic Review of Proposed Rehabilitation Guidelines Following Anatomic and Reverse Shoulder Arthroplasty
Authors: Bullock Garrett S. , Garrigues Grant E. , Ledbetter Leila , Kennedy June 
Published: April 2019

Background Total shoulder arthroplasty (TSA) is indicated for patients with glenohumeral arthritis. In this procedure, the humeral head and glenoid surface are replaced with prosthetic components. Reverse total shoulder arthroplasty (RTSA) is indicated for patients with glenohumeral arthritis and a poorly functioning rotator cuff. In this procedure, a glenosphere articulates with a humerosocket. While those surgeries are commonly performed, a thorough review of the literature is required to determine the areas of agreement and variations in postoperative rehabilitation. Objectives To describe the literature on rehabilitation protocols following anatomic TSA and RTSA. Methods For this systematic review, a computerized search was conducted in medical databases from inception to May 21, 2018 for relevant descriptive studies on TSA and RTSA rehabilitation protocols. The methodological index for nonrandomized studies tool and the modified Downs and Black tool for randomized controlled trials were used for assessment of the individual studies. Results Sixteen studies met the inclusion criteria, of which 1 provided level I evidence, 1 provided level III evidence, 2 provided level IV evidence, and 12 provided level V evidence. Ten of the studies described rehabilitation guidelines for TSA and 6 described those for RTSA. Following TSA, the use of a sling was recommended for a duration that varied from 3 to 8 weeks, and 4 of the 10 published protocols included resisted exercise during the initial stage of healing (the first 6 weeks after surgery). Seven of 10 published protocols recommended limiting shoulder external rotation to 30° and that passive range of motion be fully restored by 12 weeks post surgery. Suggested use of a sling post RTSA varied from “for comfort only” to 6 weeks, motion parameters varied from no passive range of motion to precautionary range limits, and all protocols agreed on performing deltoid isometric exercises early post surgery. There was a high level of heterogeneity for the rehabilitation guidelines and associated precautions for both TSA and RTSA. Conclusion The majority of published protocols were descriptive in nature. Published rehabilitation strategies following TSA and RTSA are based on biomechanical principles, healing time frames, and exercise loading principles, with little consistency among protocols. There is a need to determine optimal rehabilitation approaches post TSA and RTSA based on clinical outcomes. Level of Evidence Therapy, level 5. J Orthop Sports Phys Ther 2019;49(5):337–346. doi:10.2519/jospt.2019.8616
Relationship Training and Supervision with at Risk Behavior on Dump Truck's Driver
Authors: Murni Yetti , Makomulamin Makomulamin , Lapau Buchari 
Published: April 2019

At risk behavior of driver is an unsafety driving and it could harm themselves and others. One of the causes of the at risk behavior are lack of monitoring supervision, behavior of the industrial society as well as public community that haven't been optimal yet. It is 88% accident at work that caused by the unsafety behavior. This research aimed to find out the relation between knowledge, behavior, education, training, SOP and also the supervision of at Risk Behavior for dump truck's driver. This was an observational analytic quantitative study with cross sectional approach. The samples were 122 dump truck drivers. Data processing was done in the stages of editing, coding, entry, cleaning and tabulating. Data analyze was conducted by univariate, bivariate, and multivariate. The result showed that variables which has causality relation with at risk behavior, were the training (p=0,004) and the supervision (p=0,017). The confounding variable were the SOP and behavior while the education was not related to the variable. The conclusion was there is a causality relation between training and supervision with “At Risk Behavior” of dump truck’s driver. It is recommended for driver to participate in the training for work safety. It's also suggested for the supervisor to improving supervision method according to the policy and giving rewards to those drivers who's have great performance.