bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antimicrobial resistance in water in Latin America and the Caribbean: a scoping review protocol
Authors: Moreno-Switt Andrea I. , Rivera Dacil , Caipo Marisa L. , Nowell David C. , Adell Aiko D. 
Published: June 2019

ABSTRACT

Objective:
The objective of this scoping review is to identify and map existing evidence of antimicrobial resistance (AMR) in water in Latin America and the Caribbean (LAC), while also identifying the gaps in AMR information in the region in eight themes of interest.

Introduction:
Antimicrobial resistance is a public health concern that has gained increasing global awareness. Concerns have been raised toward the importance of the environment's role in the dissemination of clinically relevant AMR. Although studies on AMR have been conducted, the reality of the role of the environment in the LAC region has not been studied.

Inclusion criteria:
Articles that examine AMR in water in the LAC region will be considered for inclusion. Antimicrobial resistance will be defined as a natural process that arises when the microorganisms that cause infection (e.g. bacteria) survive exposure to a drug that would normally kill them or stop their growth. The search will focus on eight themes of interest, as defined in the protocol, relating to the presence of resistant microorganisms in water sources and reported negative health effects. Qualitative and quantitative studies will be considered for inclusion. Reviews and gray literature will be excluded.

Methods:
The proposed scoping review will be conducted in accordance with the JBI methodology for scoping reviews. A search for published literature will be performed in PubMed, Web of Science and Scopus. Independent screening of articles will be performed by examining the abstracts and then the full texts, utilizing pre-defined inclusion and exclusion criteria. Data for specific variables will be extracted, and descriptive examination will be performed.
Risk factors for hypertensive crisis in adult patients: a systematic review protocol
Authors: Benenson Irina , Waldron Frederick A. , Jadotte Yuri T. , Holly Cheryl 
Published: June 2019

ABSTRACT

Objective:
The objective of this review is to conduct comprehensive appraisal and synthesis of evidence on risk factors for hypertensive crisis and, specifically, hypertensive emergencies among adult patients with hypertension.

Introduction:
Hypertensive crisis is the most extreme form of poorly controlled hypertension that may lead to acute target organ damage (hypertensive emergency). Hypertensive crisis is associated with increased mortality, high utilization of health care and escalated healthcare costs.

Inclusion criteria:
This review will include epidemiological studies with participants over 18 years old with diagnosis of hypertension. The review will exclude pediatric, pregnant and postpartum patients. The review will consider studies that explore risk factors for hypertensive crisis, defined as an acute elevation of blood pressure equal or above 180/110 mmHg.

Methods:
The search strategy aims to find both published and unpublished studies. The databases to be searched will include MEDLINE (Ovid), Embase, Cochrane Database of Systematic Reviews and Web of Science. Following the search, all identified studies will be screened against the inclusion criteria. Selected studies will be critically appraised for methodological quality. Data on exposures and outcomes will be extracted from papers included in the review. Quantitative data, where possible, will be pooled in meta-analysis. Effect sizes expressed as odds ratio and their 95% confidence intervals will be calculated. Heterogeneity of studies will be assessed statistically. Subgroup analysis to determine the association of risk factors with hypertensive emergencies will be conducted, if possible. Where statistical pooling is not possible, the findings will be presented in a narrative form.

Systematic review registration number:
PROSPERO CRD42019140093
Inflammatory bowel disease is associated with increased gut‐to‐blood penetration of short‐chain fatty acids: A new, non‐invasive marker of a functional intestinal lesion
Authors: Jaworska Kinga , Konop Marek , Bielinska Klaudia , Hutsch Tomasz , Dziekiewicz Marcin , Banaszkiewicz Aleksandra , Ufnal Marcin 
Published: June 2019

New Findings
What is the central question of this study?
‘Leaky gut’ has been found in intestinal and extra‐intestinal diseases. However, functional evaluation of intestinal permeability is not widely used as a diagnostic marker, possibly owing to significant limitations of currently used permeability assays. There is an unmet need for development of a new, non‐invasive test to assess intestinal function.

What is the main finding and its importance?
We show that an increased blood‐to‐stool ratio of the concentration of gut bacteria‐produced short‐chain fatty acids may be used as a marker of gut permeability. Our findings lay the groundwork for establishing a new, non‐invasive, risk‐free diagnostic tool in diseases associated with intestinal barrier malfunction, such as inflammatory bowel disease.

AbstractIntestinal diseases, such as inflammatory bowel disease (IBD), are characterized by an impaired gut–blood barrier commonly referred to as ‘leaky gut’. Therefore, functional evaluation of the gut–blood barrier is a promising diagnostic marker. We hypothesized that short‐chain fatty acids (SCFAs) produced by gut bacteria might serve as a marker in IBD. Animal experiments were performed on male Sprague–Dawley rats with acetic acid‐induced colitis and in sham control animals. The gut–blood barrier permeability was determined by assessing the ratios of the following: (i) portal blood concentration of SCFAs (Cp) to faecal concentration of SCFAs (Cf); (ii) systemic blood concentration of SCFAs (Cs) to faecal concentration of SCFAs (Cf); and (iii) Cp and Cs of fluorescein isothiocyanate (FITC)–dextran administered into the colon. As a clinical study, we evaluated Cs, Cf and the Cs/Cf ratio of SCFAs in six paediatric patients with IBD, assessed as mild/moderate/severe by the Paediatric Ulcerative Colitis Activity Index (PUCAI) and the Paediatric Crohn's Disease Activity Index (PCDAI) at the time of sample collection, and nine age‐matched healthy control subjects. Rats with histologically confirmed IBD had significantly increased ratios of Cp/Cf and Cs/Cf for SCFAs. This was positively correlated with the plasma FITC–dextran concentration. Likewise, IBD patients showed a significantly higher Cs/Cf ratio for SCFAs, including acetic, valeric, isocaproic, caproic and propionic acids, in comparison to control subjects. In conclusion, in the rats and in paediatric patients with IBD we found an increased blood‐to‐stool ratio of SCFAs, suggesting an increased gut‐to‐blood penetration of SCFAs. These findings pave the way for a new, non‐invasive diagnostic tool in IBD and other diseases accompanied by intestinal barrier malfunction.
The Enhancement of Spray Cooling Performance in Nucleate and Transition Boiling Regimes by Using Saline Water Containing Dissolved Carbon Dioxide
Authors: Das Lily , Munshi B. , Mohapatra S. S. 
Published: June 2019

Abstract
In the current work, by using various additives, the spray cooling in the transition boiling regime is significantly augmented due to the vapor film instability enhancing, which helps to overcome the disadvantages reported in the open literature for the attainment of high heat flux in the aforesaid boiling regime. Saline water containing dissolved carbon dioxide produces two favorable conditions for high heat transfer rate: (1) controlled vapor bubble nucleation and (2) low entrapped vapor bubbles coalescence rate. These phenomena are the parameters defining the step-up in the heat transfer rate. Systematic spray cooling (from 900 °C) experiments were conducted on a 6-mm thick AISI 304 steel plate (100 mm × 100 mm). The heat transfer analysis indicates that the heat removal rate in case of soda added water depicts an increasing trend with the rising of the soda concentration up to 40% in water, and further increment in soda water concentration declines the heat removal rate due to the formation of the uncontrolled vapor bubbles undergoing early coalescence. In case of salt added carbonated water spray cooling, the quenching performance indicates step-up in critical heat flux up to 1.7 MW/m2. In addition to the above, the spray cooling performance of the above-stated coolant is compared with other potential coolants such as soda–surfactant–water, soda–alcohol–water and soda–salt–surfactant–water mixtures.
Relating irritability and suicidal ideation using mood and anxiety
Authors: Malhi Gin , Bell Erica , Das Pritha , Outhred Tim 
Published: June 2019

BackgroundSuicide is common in the context of depression and bipolar disorders, but there remains a lack of understanding as to how suicide ideation, a common symptom of mood disorders, progresses to suicidal behaviour. Irritability, a feature of some types of depression, is thought to contribute to the development of suicidal behaviour, but these associations are not well established.ObjectiveTo examine the relationship between irritability and suicide ideation according to the subtype of depression expressed in patients with mood disorder.Methods75 patients with mood disorders seen at the CADE (Clinical Assessment Diagnostic Evaluation) Clinic underwent clinical assessment forsuicidal ideation(Paykel Suicide Scale),symptom severity(Young Mania Rating Scale (YMRS), Hamilton Rating Scale for Depression (HAM-D) (anxious depression), Montgomery-Åsberg Depression Rating Scale (MADRS) (melancholic depression)) andirritability(item 5 of the YMRS).FindingsInterestingly, irritability correlated with mania (r=0.734, p<0.001 (YMRS)) and depressive symptom scores (r=0.369, p<0.001 (MADRS); r=0.477, p<0.001 (HAM-D)), which in turn correlated with suicide ideation scores (r=0.364, p<0.01 (MADRS); r=0.275, p=0.017 (HAM-D)). However, despite this indirect association, there was no direct correlation between irritability and suicide ideation (r=0.050, p>0.05).ConclusionsThe nature of the relationship between irritability and suicidal ideation is determined by the emotional context within which irritability operates.Clinical implicationsFindings suggest that rather than examining irritability alone, consideration of the subtype of depression, especially that of anxious depression, should be paramount in assessing suicide risk.
Cigarette prices in a complex cigarette tax system: empirical evidence from Indonesia
Authors: Prasetyo Bagus Wahyu , Adrison Vid 
Published: June 2019

BackgroundThe complex cigarette tax system in Indonesia has created the opportunity for cigarette producers to avoid tax, which results in affordable cigarette prices. In addition to increasing cigarette tax every year, the government has adopted several policies to increase cigarette prices, such as reducing the number of tiers and applying cigarette tax based on the combined production of affiliated companies (ie, firms with a minimum 10% ownership of another cigarette company, sharing the same boards of commissioners or using input from other tobacco companies, in which a firm has at least 10% ownership).ObjectiveTo investigate the effect of cigarette tax, affiliated company rules and changes in the number of tiers on cigarette prices produced in Indonesia.MethodUsing brand level data from 2005 to 2017, we model cigarette prices as a function of cigarette tax, affiliated company rules, number of tiers, cigarette types and cigarette tax system using random effect and fixed effect estimations.FindingWe find that a percentage increase in cigarette tax increases cigarette prices by less than 1% for all cigarette types. The implementation of affiliated company rules is effective in increasing cigarette prices for both affiliated and non-affiliated firms. We also find that a fewer number of tiers are associated with a higher cigarette price. Our results suggest that a reduction in the number of tiers will increase the effectiveness of a specific tax system in making cigarettes less affordable.
Evidence that an intervention weakens the relationship between adolescent electronic cigarette use and tobacco smoking: a 24-month prospective study
Authors: Conner Mark , Grogan Sarah , Simms-Ellis Ruth , Flett Keira , Sykes-Muskett Bianca , Cowap Lisa , Lawton Rebecca , Armitage Christopher , Meads David , Schmitt Laetitia , Torgerson Carole , West Robert , Siddiqi Kamran 
Published: June 2019

BackgroundThe electronic cigarette (e-cigarette) use to subsequent smoking relationship in adolescents has received much attention. Whether an intervention to reduce smoking initiation attenuated this relationship was assessed.MethodData were from 3994 adolescent never smokers (aged 13–14 years at baseline) as part of a cluster randomised controlled trial. Self-report measures of smoking, e-cigarette use and covariates were assessed and used to predict ever smoked cigarettes, any recent tobacco smoking and regularly smoked cigarettes at 24-month follow-up.ResultsBaseline ever use of e-cigarettes was associated with ever smoked cigarettes (OR=4.03, 95% CI 3.33 to 4.88; controlling for covariates, OR=2.78, 95% CI 2.20 to 3.51), any recent tobacco smoking (OR=3.38, 95% CI 2.72 to 4.21; controlling for covariates, OR=2.17, 95% CI 1.76 to 2.69) and regularly smoked cigarettes (OR=3.60, 95% CI 2.35 to 5.51; controlling for covariates, OR=1.27, 95% CI 1.17 to 1.39) at follow-up. For ever smoked cigarettes only, the impact of e-cigarette use was attenuated in the intervention (OR=1.83) compared with control (OR=4.53) condition. For ever smoked cigarettes and any recent tobacco smoking, the impact of e-cigarette use was attenuated among those with friends who smoked (OR=2.05 (ever smoked); 1·53 (any tobacco use)) compared with those without friends who smoked (OR=3.32 (ever smoked); 2·17 (any tobacco use)).ConclusionsThis is one of the first studies to show that e-cigarette use was robustly associated with measures of smoking over 24 months and the first to show an intervention to attenuate the relationship. Further research with a broader age range of adolescents is required.
Rational synthesis of graphitic porous carbon with high content nitrogen doping via ultra-fast pyrolysis of ZIF-8 for electrochemical capacitor with enhanced performance
Authors: Gao Tie , Li Haibo 
Published: June 2019

In this work, we proposed an effective strategy to prepare nitrogen-doped popcorn-like porous carbons (NPPCs) via ultra-fast carbonization of zeolitic immidazolate frameworks (ZIFs-8), where the ZIFs-8 acted as carbon precursor as well as the template. The obtained NPPCs possess popcorn-like morphology with large specific surface area of 1243[Formula: see text]m2/g, total pore volume of 1.48[Formula: see text]cm3/g and high nitrogen content. Remarkably, the average pore diameter of NPPCs was 4.72[Formula: see text]nm, indicating the presence of amount substantial mesopores. As the electrode of supercapacitor, the NPPCs revealed a relatively high specific capacitance of 610.4[Formula: see text]F/g in KOH (6[Formula: see text]mol/L) at 5[Formula: see text]mV/s. Even the scan rate was increased to 50[Formula: see text]mV/s, an impressive capacity of 424.8[Formula: see text]F/g can be achieved, suggesting good rate capability. Besides, it exhibited outstanding cycling stability with 93% of specific capacitance retention after 10,000 GCD cycles. Moreover, the NPPCs electrode demonstrated high electrochemical performance and stability by designing the coin-type and flexible supercapacitor. The large specific surface area, abundant accessible mesoporosity and novel nanostructure are account for the superior performance.
Decompositions of some regular graphs into unicyclic graphs of order five
Authors: Paulraja P. , Sivakaran T. 
Published: June 2019

For a graph [Formula: see text] and a subgraph [Formula: see text] of [Formula: see text] an [Formula: see text]-decomposition of [Formula: see text] is a partition of the edge set of [Formula: see text] into subsets [Formula: see text] [Formula: see text] such that each [Formula: see text] induces a graph isomorphic to [Formula: see text] It is proved that the necessary conditions are sufficient for the existence of an [Formula: see text]-decomposition of the graph [Formula: see text] where [Formula: see text] is any simple connected unicyclic graph of order five, × denotes the tensor product of graphs and [Formula: see text] denotes the multiplicity of the edges. In fact, using the above characterization, a necessary and sufficient condition for the graph [Formula: see text] [Formula: see text] and [Formula: see text] to admit an [Formula: see text]-decomposition is obtained. Similar results for the complete graphs and complete multipartite graphs are proved in: [J.-C. Bermond et al. [Formula: see text]-decomposition of [Formula: see text], where [Formula: see text] has four vertices or less, Discrete Math. 19 (1977) 113–120, J.-C. Bermond et al. Decomposition of complete graphs into isomorphic subgraphs with five verices, Ars Combin. 10 (1980) 211–254, M. H. Huang, Decomposing complete equipartite graphs into connected unicyclic graphs of size five, Util. Math. 97 (2015) 109–117].
Cell-Cycle–Dependent Phosphorylation of PRPS1 Fuels Nucleotide Synthesis and Promotes Tumorigenesis
Authors: Jing Xiaoqian , Wang Xiong-jun , Zhang Tao , Zhu Wencheng , Fang Yi , Wu Haoxuan , Liu Xinyu , Ma Ding , Ji Xiaopin , Jiang Yimei , Liu Kun , Chen Xianze , Shi Yi , Zhang Yaqi , Shi Minmin , Qiu Weihua , Zhao Ren 
Published: June 2019

Abstract

Nucleotide supply is essential for DNA replication in proliferating cells, including cancer cells. Ribose-phosphate diphosphokinase 1 (PRPS1) is a key enzyme to produce the consensus precursor of nucleotide synthesis. PRPS1 participates in the pentose phosphate pathway (PPP) by catalyzing the phosphoribosylation of D-ribose 5-phosphate (R-5P) to 5-phosphoribosyl-1-pyrophosphate. Therefore, PRPS1 not only controls purine biosynthesis and supplies precursors for DNA and RNA biosynthesis but also regulates PPP through a feedback loop of the PRPS1 substrate R-5P. However, it is still elusive whether PRPS1 enhances nucleotide synthesis during cell-cycle progression. In this study, we explore the role and activation mechanism of PRPS1 in cell-cycle progression of colorectal cancer, and observed a peak in its enzymatic activity during S phase. CDK1 contributes to upregulation of PRPS1 activity by phosphorylating PRPS1 at S103; loss of phosphorylation at S103 delayed the cell cycle and decreased cell proliferation. PRPS1 activity in colorectal cancer samples is higher than in adjacent tissue, and the use of an antibody that specifically detects PRPS1 phosphorylation at S103 showed consistent results in 184 colorectal cancer tissues. In conclusion, compared with upregulation of PRPS1 expression levels, increased PRPS1 activity, which is marked by S103 phosphorylation, is more important in promoting tumorigenesis and is a promising diagnostic indicator for colorectal cancer.

Significance:
These findings show that the enzymatic activity of PRPS1 is crucial for cell-cycle regulation and suggest PRPS1 phosphorylation at S103 as a direct therapeutic target and diagnostic biomarker for colorectal cancer.
Monomer Preference of EGFR Tyrosine Kinase Inhibitors Influences the Synergistic Efficacy of Combination Therapy with Cetuximab
Authors: Oashi Ayano , Yasuda Hiroyuki , Kobayashi Keigo , Tani Tetsuo , Hamamoto Junko , Masuzawa Keita , Manabe Tadashi , Terai Hideki , Ikemura Shinnosuke , Kawada Ichiro , Naoki Katsuhiko , Soejima Kenzo 
Published: June 2019

Abstract
EGFR-mutated lung cancer is a significant subgroup of non–small cell lung cancer. To inhibit EGFR-mediated signals, multiple EGFR tyrosine kinase inhibitors (EGFR-TKI) have been developed; however, approximately one third of patients with EGFR-mutated lung cancer do not respond to EGFR-TKIs. More effective inhibition of EGFR-mediated signals is therefore necessary. For cancers expressing mutated EGFR, including EGFR T790M, which confers resistance to first- (gefitinib and erlotinib) and second- (afatinib) generation EGFR-TKIs, the synergistic efficacy of afatinib and cetuximab combination therapy has been reported in preclinical and clinical studies; however, the mechanisms underlying this effect remain elusive. In this study, we evaluated the effects of multiple EGFR-TKIs on the EGFR monomer–dimer equilibrium by inducing dimerization-impairing mutations in cells expressing EGFR. Interestingly, we found that afatinib and dacomitinib exhibit a monomer preference: cells expressing dimerization-impaired EGFR mutants exhibited increased sensitivity to afatinib and dacomitinib relative to those with dimerization-competent EGFR mutants. Although EGFR-TKIs themselves induce dimerization of EGFR, the inhibition of dimerization by cetuximab overcame EGFR-TKI–induced dimerization. By shifting the monomer–dimer equilibrium toward monomer dominance using cetuximab, the effectiveness of afatinib and dacomitinib improved significantly. We report a novel and clinically relevant phenomenon, the monomer preference of EGFR-TKIs, which can explain the mechanism underlying the synergism observed in afatinib and cetuximab combination therapy. In addition, we propose the novel concept that monomer–dimer equilibrium is an important factor in determining EGFR-TKI efficacy. These findings provide novel insights into treatment strategies for EGFR-TKI–refractory non–small cell lung cancer.
Regulation of health professions in Ontario: self-regulation with statutory- based public accountability
Authors: Lemmens Trudo , Ghimire Kanksha Mahadevia 
Published: June 2019

The paper explores the model of regulation of health professionals in Ontario, Canada; a self-regulation model built around a detailed statutory scheme. The core of the paper consists of a discussion of Ontario’s Regulated Health Professions Act and of the key components of 26 specific health profession acts that have been enacted under its umbrella. The paper explores the role of the regulatory colleges, the role of the Ministry of Health in determining scope of practice and other components of medical practice, and the disciplinary and appeal procedures. Some other specific issues are also briefly touched upon, such as the integration into the profession of internationally trained physicians, and the government’s role in ensuring access to specialists across the province. A final section looks at the challenges and the limitations of the Ontario model, through a number of health professions-related controversies that reveal gaps in self-regulation, including: failure to set and enforce proper educational and practice standards in specific areas; failure to conduct timely investigations into potential misconduct by professionals; and failure to question professionals in a position of power. The paper also discusses briefly the implications of recognizing through legal regulation some alternative and complementary medical practices, and the challenge of regulating indigenous health care practitioners. It concludes that the primary limitations of the regulatory model arise on account of professional self-interest and power-relations impacting procedural issues, and the complexity of the regulatory model that may potentially undermine quality control.
Saúde não é mercadoria
Authors: Dallari Sueli Gandolfi 
Published: June 2019

Exatamente porque saúde não é mercadoria, a regulação sanitária ganha relevo no mundo capitalista. O atual escândalo conhecido como Implant Files é suficiente para demonstrar que o apetite das empresas atuantes nessa área é insaciável e que, sem adequada regulação e atenta vigilância sobre elas, serão altamente prejudicados não só os pacientes (no caso, com sequelas provocadas por produtos de baixa qualidade e inadequados à necessidade), mas também os sistemas de saúde, que além de arcarem com os custos decorrentes da compra dos implantes, devem suportar os encargos de cuidar das sequelas e de eventual indenização.