bluuebunny/crossref_metadata_2025_split huggingface dataset:
The supplementary motor area modulates interhemispheric interactions during movement preparation
Authors: Welniarz Quentin , Gallea Cécile , Lamy Jean‐Charles , Méneret Aurélie , Popa Traian , Valabregue Romain , Béranger Benoît , Brochard Vanessa , Flamand‐Roze Constance , Trouillard Oriane , Bonnet Cécilia , Brüggemann Norbert , Bitoun Pierre , Degos Bertrand , Hubsch Cécile , Hainque Elodie , Golmard Jean‐Louis , Vidailhet Marie , Lehéricy Stéphane , Dusart Isabelle , Meunier Sabine , Roze Emmanuel 
Published: January 2019

AbstractThe execution of coordinated hand movements requires complex interactions between premotor and primary motor areas in the two hemispheres. The supplementary motor area (SMA) is involved in movement preparation and bimanual coordination. How the SMA controls bimanual coordination remains unclear, although there is evidence suggesting that the SMA could modulate interhemispheric interactions. With a delayed‐response task, we investigated interhemispheric interactions underlying normal movement preparation and the role of the SMA in these interactions during the delay period of unimanual or bimanual hand movements. We used functional MRI and transcranial magnetic stimulation in 22 healthy volunteers (HVs), and then in two models of SMA dysfunction: (a) in the same group of HVs after transient disruption of the right SMA proper by continuous transcranial magnetic theta‐burst stimulation; (b) in a group of 22 patients with congenital mirror movements (CMM), whose inability to produce asymmetric hand movements is associated with SMA dysfunction. In HVs, interhemispheric connectivity during the delay period was modulated according to whether or not hand coordination was required for the forthcoming movement. In HVs following SMA disruption and in CMM patients, interhemispheric connectivity was modified during the delay period and the interhemispheric inhibition was decreased. Using two models of SMA dysfunction, we showed that the SMA modulates interhemispheric interactions during movement preparation. This unveils a new role for the SMA and highlights its importance in coordinated movement preparation.
Transoral robotic excision of laryngocele: Surgical considerations
Authors: Patel Krupal B. , Lin Chen , Kramer Scott , Fada Craig , Ozer Enver 
Published: January 2019

AbstractBackgroundTransoral robotic surgery (TORS) has emerged as a novel, safe, and feasible procedure for the resection of malignant supraglottic laryngeal cancers. The purpose of this study was to demonstrate the surgical technique with extension of the use of TORS to excise a laryngeal cyst.ResultsLaryngeal cyst resection, along with its tract, was accomplished with preservation of both false and true vocal cords. There was no perioperative or early postoperative complications. The patient was extubated immediately after surgery. Oral diet was initiated within 24 hours. No tracheostomy was required. A video demonstration of the surgical technique is included on Head & Neck's website.ConclusionTORS is a safe and feasible procedure for excision of selected laryngeal cysts.
Prognostic factors in recurrent or metastatic squamous cell carcinoma of the head and neck
Authors: Mirabile Aurora , Miceli Rosalba , Calderone Rosa Gioia , Locati Laura , Bossi Paolo , Bergamini Cristiana , Granata Roberta , Perrone Federica , Mariani Luigi , Licitra Lisa 
Published: January 2019

AbstractBackgroundIn clinical trials of recurrent and metastatic head and neck carcinoma, Argiris et al have identified prognostic factors for overall survival (OS) and progression‐free survival (PFS), weight loss, Eastern Cooperative Oncology Group performance status (ECOG‐PS), tumor primary site, tumor differentiation, prior radiotherapy, deriving a two‐group prognostic classification. This study evaluates Argiris's classification in “field‐practice” patients.MethodsThe main analysis included 327 cases; a secondary analysis excluded 31 patients with oropharyngeal carcinoma (OPC) p16‐positive and/or human papilloma virus (HPV)‐positive. OS and PFS curves were estimated with the Kaplan‐Meier method; multivariable Cox analyses were also performed.ResultsIn the full series, OS was significantly different in patients with 0‐2 and ≥3 adverse features (median, 14 vs 10 months; P = .03). PFS was statistically different in the two groups (median, 7 vs 5 months; P = .02). At a multivariable analysis investigating additional prognostic features, site of relapse and disease‐free interval were significant predictors of OS and PFS.ConclusionThe Argiris's model was confirmed in a “field‐practice” population. Moreover, we found additional putative prognostic factors.
Podocan affects C2C12 myogenic differentiation by enhancing Wnt/β‐catenin signaling
Authors: Liu Dan , Li Shuang , Cui Yafeng , Tong Huili , Li Shufeng , Yan Yunqin 
Published: January 2019

AbstractPodocan, a small leucine‐rich repeat protein, is a negative regulator of cell proliferation. In this study, we demonstrated that podocan is involved in the differentiation of C2C12 murine myoblasts. Podocan expression increased with the progression of C2C12 differentiation. As expect, siRNA‐mediated podocan knockdown inhibited C2C12 differentiation, as indicated by inhibition of MYOG, MYH2, and desmin expression, as well as reductions in the differentiation and fusion indices. Overexpression of podocan using dCas9 technology promoted C2C12 cell differentiation. In addition, supplementation of culture medium with podocan influenced C2C12 differentiation. Podocan knockdown reduced Wnt/β‐catenin signaling, characterized by a reduction in the nuclear translocation of β‐catenin, whereas podocan overexpression had the opposite effect. Furthermore, treatment with XAV939, an inhibitor of Wnt/β‐catenin, reduced the podocan‐mediated promotion of C2C12 differentiation. Induction of muscle injury in mice by bupivacaine administration suggested that podocan may play a role in muscle regeneration. In summary, our results suggest that podocan is required for normal C2C12 differentiation and that its role in myogenesis is mediated by the Wnt/β‐catenin pathway.
SP1‐SYNE1‐AS1‐miR‐525‐5p feedback loop regulates Ang‐II‐induced cardiac hypertrophy
Authors: Wang Ye , Cao Rongyi , Yang Wenwen , Qi Bangruo 
Published: January 2019

AbstractCardiac hypertrophy (CH) has become a huge threat to human health. Recent years, long noncoding RNAs (lncRNAs) have been studied in human diseases, including CH. According to bioinformatics analysis, 10 lncRNAs possibly involved in the progression of CH were screened out. Among which, lncRNA SYNE1 antisense RNA 1 (SYNE1‐AS1) could be upregulated by Angiotensin II (Ang‐II) in cardiomyocytes. Thus, we chose SYNE1‐AS1 to do further study. To identify the biological function of SYNE1‐AS1 in CH, SYNE1‐AS1 was silenced in Ang‐II‐induced cardiomyocytes. Results of immunofluorescence staining demonstrated that increased cell surface area in Ang‐II‐induced cardiomyocytes was reduced by SYNE1‐AS1 knockdown. Moreover, the hypertrophic responses were attenuated by SYNE1‐AS1 knockdown. Mechanically, SYNE1‐AS1 positively regulated Sp1 transcription factor (SP1) by sponging microRNA‐525‐5p (miR‐525‐5p). On the basis of previous reports, SP1 can transcriptionally activate lncRNAs. Therefore, we investigated the interaction between SP1 and SYNE1‐AS1 promoter. Intriguingly, SYNE1‐AS1 was activated by SP1. At last, rescue assays demonstrated the function of SP1‐SYNE1‐AS1 axis in CH. In conclusion, SP1‐induced upregulation of lncRNA SYNE1‐AS1 promoted CH via miR‐525‐5p/SP1 axis.
Epigenetic mechanisms are behind the regulation of the key genes associated with the osteoblastic differentiation of the mesenchymal stem cells: The role of zoledronic acid on tuning the epigenetic changes
Authors: Marofi Faroogh , Hassanzadeh Ali , Solali Saeed , Vahedi Ghasem , Mousavi Ardehaie Reza , Salarinasab Sadegh , Aliparasti Mohammad Reza , Ghaebi Mahnaz , Farshdousti Hagh Majid 
Published: January 2019

AbstractMesenchymal stem cells (MSCs) are multipotent stem cells and show distinct features such as capability for self‐renewal and differentiation into several lineages of cells including osteoblasts, chondrocytes, and adipocytes. In this study, the methylation status of the promoter region of zinc finger and BTB domain containing 16 (ZBTB16), twist‐related protein 1(Twist1), de novo DNA methyltransferases 3A (DNMT3A), SRY‐box 9 (Sox9), osteocalcin (OCN), and peroxisome proliferator‐activated receptor γ2 (PPARγ2) genes and their messenger RNA (mRNA) expression levels were evaluated during the osteoblastic differentiation of MSCs (ODMSCs). We planned two experimental groups including zoledronic acid (ZA)‐treated and nontreated cells (negative control) which both were differentiated into the osteoblasts. Methylation level of DNA in the promoter regions was assayed by methylation‐specific‐quantitative polymerase chain reaction (MS‐qPCR), and mRNA levels of the target inhibitory/stimulatory genes during osteoblastic differentiation of MSCs were measured using real‐time PCR. During the experimental induction of ODMSCs, the mRNA expression of the OCN gene was upregulated and methylation level of its promoter region was decreased. Moreover, Sox9 and PPARγ2 mRNA levels were attenuated and their promoter regions methylation levels were significantly augmented. However, the mRNA expression of the DNMT3A was not affected during the ODMSCs though its methylation rate was increased. In addition, ZA could enhance the expression of the ZBTB16 and decrease its promoter regions methylation and on the opposite side, it diminished mRNA expression of Sox9, Twist1, and PPARγ2 genes and increased their methylation rates. Intriguingly, ZA did not show a significant impact on gene expression and methylation levels the OCN and DNMT3A. We found that methylation of the promoter regions of Sox9, OCN, and PPARγ2 genes might be one of the main mechanisms adjusting the genes expression during the ODMSCs. Furthermore, we noticed that ZA can accelerate the MSCs differentiation to the osteoblast cells via two regulatory processes; suppression of osteoblastic differentiation inhibitor genes including Sox9, Twist1, and PPARγ2, and through promotion of the ZBTB16 expression.
Identification of differentially expressed genes and biological characteristics of colorectal cancer by integrated bioinformatics analysis
Authors: Sun Guangwei , Li Yalun , Peng Yangjie , Lu Dapeng , Zhang Fuqiang , Cui Xueyang , Zhang Qingyue , Li Zhuang 
Published: January 2019

AbstractColorectal cancer (CRC) ranks as one of the most common malignant tumors worldwide. Its mortality rate has remained high in recent years. Therefore, the aim of this study was to identify significant differentially expressed genes (DEGs) involved in its pathogenesis, which may be used as novel biomarkers or potential therapeutic targets for CRC. The gene expression profiles of GSE21510, GSE32323, GSE89076, and GSE113513 were downloaded from the Gene Expression Omnibus (GEO) database. After screening DEGs in each GEO data set, we further used the robust rank aggregation method to identify 494 significant DEGs including 212 upregulated and 282 downregulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed by DAVID and the KOBAS online database, respectively. These DEGs were shown to be significantly enriched in different cancer‐related functions and pathways. Then, the STRING database was used to construct the protein–protein interaction network. The module analysis was performed by the MCODE plug‐in of Cytoscape based on the whole network. We finally filtered out seven hub genes by the cytoHubba plug‐in, including PPBP,
CCL28,
CXCL12,
INSL5,
CXCL3,
CXCL10, and
CXCL11. The expression validation and survival analysis of these hub genes were analyzed based on The Cancer Genome Atlas database. In conclusion, the robust DEGs associated with the carcinogenesis of CRC were screened through the GEO database, and integrated bioinformatics analysis was conducted. Our study provides reliable molecular biomarkers for screening and diagnosis, prognosis as well as novel therapeutic targets for CRC.
Co3(PO4)2/Ag3PO4 with enhanced simulated sunlight photocatalytic activity toward ofloxacin degradation and mechanism insight
Authors: Geng Zhi , Yang Min , Qi Xin , Li Zhaoyang , Yang Xia , Huo Mingxin , Crittenden John C 
Published: January 2019

AbstractBACKGROUNDOfloxacin is a frequently detected fluoroquinolone antibiotic in wastewater treatment plant effluents, sea waters and surface waters. Photocatalytic technology is considered to be the most promising treatment technology for the removal of ofloxacin. However, it is a big challenge to exploit a novel sunlight‐driven photocatalyst and reveal the mechanism of ofloxacin degradation.RESULTSCo3(PO4)2/Ag3PO4 composites were prepared using a facile hydrothermal synthesis method. The structural, morphological and optical properties of the composites were well characterized. Both the degradation efficiency and cyclical stability of the Co3(PO4)2/Ag3PO4 composites increased significantly under simulated sunlight irradiation when ofloxacin (OFX) or methyl orange (MO) was used as the target molecule, as compared with single‐phase Ag3PO4 and Co3(PO4)2. The reduction of antimicrobial activity for 8%Co3(PO4)2/Ag3PO4 reached 88.8% after 5 min of sunlight irradiation.CONCLUSIONCo3(PO4)2 played a critical role in suppressing carrier recombination and provided a large number of photogenerated holes and •O2− to oxidize OFX or MO. The OFX degradation mechanism included piperazinyl dealkylation, decarboxylation and defluorination. The reduction of antimicrobial activity for degradation byproducts was obvious after simulated sunlight irradiation over Co3(PO4)2/Ag3PO4. Therefore Co3(PO4)2/Ag3PO4 is an attractive candidate for the removal of OFX. © 2019 Society of Chemical Industry
Pitch control and speed limitation during overground deceleration in lemurid primates
Authors: Miller Charlotte E. , Pinkard Henry , Johnson Laura E. , Schmitt Daniel 
Published: January 2019

AbstractAn animal's fitness is influenced by the ability to move safely through its environment. Recent models have shown that aspects of body geometry, for example, limb length and center of mass (COM) position, appear to set limits for pitch control in cursorial quadrupeds. Models of pitch control predict that the body shape of these and certain other primates, with short forelimbs and posteriorly positioned COM, should allow them to decelerate rapidly while minimizing the risk of pitching forward. We chose to test these models in two non‐cursorial lemurs: Lemur catta, the highly terrestrial ring‐tailed lemur, and Eulemur fulvus, the highly arboreal brown lemur. We modeled the effects of changes in limb length and COM position on maximum decelerative potential for both species, as well as collecting data on maximal decelerations across whole strides. In both species, maximum measured decelerations fell below the range of pitch‐limited deceleration values predicted by the geometric model, with the ring‐tailed lemur approaching its pitch limit more closely. Both lemurs showed decelerative potential equivalent to or higher than horses, the only comparative model currently available. These data reinforce the hypothesis that a relatively simple model of body geometry can predict aspects of maximum performance in animals. In this case, it appears that the body geometry of primates is skewed toward avoiding forward pitch in maximal decelerations.
3D‐ASL perfusion correlates with VEGF expression and overall survival in glioma patients: Comparison of quantitative perfusion and pathology on accurate spatial location‐matched basis
Authors: Pang Haopeng , Dang Xuefei , Ren Yan , Zhuang Dongxiao , Qiu Tianming , Chen Hong , Zhang Jie , Ma Ningning , Li Gang , Zhang Junhai , Wu Jinsong , Feng Xiaoyuan 
Published: January 2019

BackgroundThere is a need for an imaging‐based tool for measuring vascular endothelial growth factor (VEGF) expression and overall survival (OS) in patients with glioma.PurposeTo assess the correlation between cerebral blood flow (CBF), measured by 3D pseudo‐continuous arterial spin‐labeling (3D‐ASL), and VEGF expression in gliomas on the basis of coregistered localized biopsy, and investigate whether CBF correlated with survival month (SM) in glioma patients.Study TypeProspective cohort.SubjectsThirty‐seven patients with gliomas from whom 63 biopsy specimens were obtained.Sequence3D‐ASL acquired with a 3.0T MR unit.AssessmentBiopsy specimens were grouped as high‐grade (HGG) or low‐grade glioma (LGG). CBF measurements were spatially matched with VEGF expression by coregistered localized biopsies, and the CBF value was correlated with quantitative VEGF expression for each specimen. Patients' survival information was derived and connected with CBF.Statistical TestsPatients' OS was analyzed by Kaplan–Meier and Cox‐regression methods. VEGF expression and CBF were compared in both LGG and HGG. The Spearman rank correlation was calculated for CBF and VEGF expression, SM. Significance level, P < 0.05.ResultsCBF‐derived 3D‐ASL positively correlated significantly with VEGF expression in both LGG (31 specimens) and HGG (32 specimens), r = 0.604 (P < 0.001) and r = 0.665 (P < 0.001), respectively. LGG and HGG together gave a correlation coefficient r = 0.728 (P < 0.001). Median survival for LGG and HGG patients was 34.19 and 17.17 months, respectively (P = 0.037); CBF value negatively correlated significantly with SM with r = –0.714 (P < 0.001) regardless of glioma grade. CBF was an independent risk factor for OS with HR = 1.027 (P = 0.044), 1.028 (P = 0.010) for univariate/multivariate regression analysis.Data ConclusionCBF determined by 3D‐ASL correlates with VEGF expression in glioma and is an independent risk factor for OS in these patients.Level of Evidence: 1Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2019;50:209–220.
Integration of Neural Reward Processing and Appetite‐Related Signaling in Obese Females: Evidence From Resting‐State fMRI
Authors: Zhang Peng , Liu Yang , Lv Han , Li Meng‐yi , Yu Feng‐xia , Wang Zheng , Ding He‐yu , Wang Li‐xue , Zhao Kai‐xin , Zhang Zheng‐yu , Zhao Peng‐fei , Li Jing , Yang Zheng‐han , Zhang Zhong‐tao , Wang Zhen‐chang 
Published: January 2019

BackgroundThe reward‐related regions have been considered a crucial component in the regulation of eating behavior. Furthermore, appetite‐related regions associated with reward can influence eating behaviors through altered functional activity related to food in brain areas associated with emotion, memory, sensory processing, motor function, and cognitive control.PurposeTo investigate the key nodes in obese females of reward‐related regions and, based on key nodes, to evaluate the directionality of functional connectivity between key nodes and appetite‐related regions.Study TypeProspective.PopulationTwenty‐eight obese and 28 normal‐weight female controls of similar age.Field Strength/Sequence3.0 T MRI and echo planar imaging (EPI) sequence, 3D BRAVO sequence.AssessmentThe fMRI data preprocessing was based on the Data Processing & Analysis of Brain Imaging and Statistical Parametric Mapping 12. Degree centrality calculation was based on the GRETNA toolkit and granger causality analysis were based on the DynamicBC toolbox.Statistical Tests: Independent two‐sample t‐tests were used to assess the differences in demographic and clinical data between two groups. Two‐sample t‐tests were conducted to test the difference in degree centrality and effective connectivity of key nodes between two groups.ResultsCompared with normal‐weight controls, obese females showed an increased degree centrality in the left ventral striatum/caudate (t = 2.96808, P < 0.05) and decreased degree centrality in right orbitofrontal cortex (OFC) (t = –3.3558, P < 0.05). The obese females showed directional effective connectivity between left ventral striatum/caudate and several regions (left inferior temporal gyrus, fusiform gyrus, postcentral gyrus, and right precentral gyrus) (P < 0.05). Directional effective connectivity was also observed between the right OFC and several regions (left middle temporal gyrus, cuneus, OFC, superior temporal gyrus, middle frontal gyrus, and right inferior parietal lobule) (P < 0.05).Data ConclusionThe left ventral striatum/caudate and right OFC are key nodes in reward‐related regions. The key nodes with reward processing mainly enhance visual processing of information and further participate in cognitive, attention, and sensorimotor processing.Level of Evidence: 1.Technical Efficacy: Stage 4.J. Magn. Reson. Imaging 2019;50:541–551.
Downscaling climate change of water availability, sediment yield and extreme events: Application to a Mediterranean climate basin
Authors: Zhang Rong , Corte‐Real João , Moreira Madalena , Kilsby Chris , Birkinshaw Stephen , Burton Aidan , Fowler Hayley J. , Forsythe Nathan , Nunes João Pedro , Sampaio Elsa , dos Santos Francisco Lúcio , Mourato Sandra 
Published: January 2019

A robust hydrological impact assessment is indispensable for mitigation and adaptation planning. This study presents an integrated modelling methodology for evaluating climate change impacts on water availability, sediment yield and extreme events at the catchment scale. We propose the use of the spatial–temporal Neyman–Scott Rectangular Pulses (STNSRP) model—RainSim V3 and the rainfall conditioned daily weather generator—ICAAM‐WG, as well as the physically based spatially distributed hydrological model—SHETRAN. The change factor approach was applied for obtaining unbiased rainfall and temperature statistics. The ICAAM‐WG was developed based on the modified Climate Research Unit daily Weather Generator (CRU‐WG). The methodology is proposed to generate synthetic series of hourly precipitation, daily temperature and potential evapotranspiration, hourly runoff and hourly sediment discharge. We demonstrated a possible application in a 705‐km2 Mediterranean climate basin in southern Portugal. The case study showed the evaluation of future climate change impacts on annual and monthly water balance components and sediment yield, annual and seasonal flow duration curves, empirical extreme value distributions and the theoretical fits. It did not consider the possible uncertainty due to the limit of computational resources.The methodology can be well justified as follows: (a) the use of synthetic hourly instead of daily precipitation enables SHETRAN to be more capable of reproducing reliable storm runoff processes and the consequent sediment transport processes; (b) the use of SHETRAN makes possible the impact assessment to be accessible for any model grid square within the study basin; (c) the use of a statistical–stochastic downscaling method facilitates the generation of the synthetic series with unlimited length. It makes possible robust hydrological impact assessments if uncertainties related to the global climate model, regional climate model, greenhouse gas emission scenario, downscaling method, hydrological model and observational data are considered.