bluuebunny/crossref_metadata_2025_split huggingface dataset:
Respiratory syncytial virus vaccination among US adults aged ≥60 years
Authors: Geng Xiaozhen , Wang Wenjun 
Published: December 2024

BackgroundRespiratory syncytial virus (RSV) vaccines have been recommended for US adults aged ≥60 years for nearly one year. However, the extent of vaccination coverage and the factors influencing uptake remain underexplored. This study aimed to evaluate national and state-specific RSV vaccination coverage among US adults aged ≥60 years and to identify demographic and socioeconomic factors that influence vaccination uptake.MethodsThis cross-sectional study analyzed data from the US Census Household Pulse Survey, from January 9 to March 4, 2024, which included 49,322 adults aged ≥60 years. Participants self-reported their RSV vaccination status along with demographic and socioeconomic information. Multivariable Poisson regression was used to identify factors associated with vaccination uptake.ResultsAs of March 4, 2024, RSV vaccination coverage among US adults aged ≥60 years was estimated at 22.2% (95% CI, 21.6%-22.7%). State-specific vaccination coverage ranged from 12.4% in Mississippi to 33.1% in Colorado. Coverage was higher in older age groups, with 21.2% among those aged 65-69 years, 28.1% among 70-74 years, 31.9% among 75-79 years, and 30.2% among those aged ≥80 years, compared to 10.8% among those aged 60-64 years. Racial/ethnicity disparities were evident, with lower coverage among Hispanics (18.3%) and non-Hispanic Blacks (17.6%) compared to non-Hispanic Whites (23.3%). Coverage was similar between males (22.6%) and females (21.8%). Factors positively associated with vaccination included older age, higher education level, higher household income, unemployment, and residing in the Midwest or West. Conversely, being non-Hispanic Black, uninsured, experiencing financial difficulties, and lack of COVID-19 vaccination were associated with reduced likelihood of receiving the RSV vaccine.ConclusionsDuring the first RSV season after the recommendations for RSV vaccination in US adults aged ≥60 years, uptake shows promise but is marked by significant disparities related to race/ethnicity, socioeconomic status, and geographic location. Efforts to address these disparities are crucial to enhance vaccination coverage and reduce the impact of RSV on this vulnerable population.
Optimizing antifungal therapy: a systematic review of pharmacist interventions, stewardship approaches, and outcomes
Authors: Akbar Zunaira , Aamir Muhammad , Saleem Zikria 
Published: December 2024

IntroductionSpecific evidence regarding the pharmacist’s role in antifungal stewardship (AFS) is emerging. This review aims to identify pharmacist-driven AFS interventions to optimize antifungal therapy.MethodsA systematic review was conducted using Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Data (2018–2023) were collected through Google Scholar and PubMed. The collected data were presented descriptively due to variations in interventions and outcome metrics. Conclusions were derived through a qualitative synthesis of the identified findings.ResultsA total of 232 articles were retrieved, and after applying inclusion and exclusion criteria, 27 were included in the review. Among the eight studies evaluating the impact of pharmacist interventions on antifungal consumption, 6 studies reported a significant decline in defined daily dose (DDD)/1,000 patient days and days of therapy (DOT)/1,000 patient days, one reported a non-significant decrease, and one reported an increase in the utilization of echinocandins. Educational intervention was the most commonly used stewardship approach. Nineteen studies reported data on various clinical outcomes. Mortality and length of hospital stay remain non-significant, but the occurrence of ADR decreased significantly, and the quality of antifungal use improved significantly.ConclusionPharmacist-led AFS has the potential to enhance the effectiveness of antifungal treatments by improving their overall quality, reduction in consumption, and adverse events. The healthcare system should encourage multidisciplinary collaboration where pharmacists play a central role in decision-making processes regarding antifungal use.
Excessive health behaviors in sports: links of orthorexia nervosa and exercise addiction with well-being, exercise activity in sports categories, and gender effects
Authors: Wachten Hanna , Wurst Ramona , Paganini Sarah , Strahler Jana 
Published: December 2024

Background and aimsOrthorexia nervosa (OrNe), the obsessive fixation on healthy eating, and exercise addiction (ExAdd) have been discussed as correlated excessive health behaviors with potential mental health implications. The role of gender-and sports-specific differences remains unclear. This study aimed to investigate the links of ExAdd, OrNe, and the non-pathological interest in healthy eating (healthy orthorexia; HeOr) with well-being and exercise activity in various sports, considering gender effects.MethodsData from two cross-sectional online surveys were combined (N = 1,064, 73.5% women, age = 28.23 ± 11.09), measuring ExAdd (Exercise Addiction Inventory Revised), OrNe and HeOr (Teruel Orthorexia Scale), well-being (WHO-5 Well-Being Index), exercise activity and sports (Physical Activity, Exercise, and Sport Questionnaire).ResultsWell-being correlated with OrNe among women (r = −0.291) and not meaningfully with ExAdd and HeOr among men. Gender differences were not significant, except for the higher correlation of ExAdd with exercise activity in resistance and fitness sports among men. Regression analyses revealed that exercise activity, especially in health, resistance and fitness sports, was linked to HeOr, but not to OrNe. ExAdd was associated with exercise activity in endurance, resistance and fitness, ball and team, antigravitation, technical, aesthetic sports, and martial arts.DiscussionMen’s well-being may slightly benefit from ExAdd and an interest in healthy eating, while obsessive healthy eating may reduce women’s well-being. Although exercise activity is associated with ExAdd in a wide variety of sports, it does not explain the link between ExAdd and OrNe. Preventive measures should consider gender-specific risks in excessive health behaviors.
CircRNA_036186 mediates HNSCC progression by regulating 14-3-3ζ
Authors: Tang Juan , Yu Donglin , Song Jiaojiao , Li Junfei , Zhang Yijuan , Ma Xiangrui , Wang Wenlong 
Published: December 2024

IntroductionHead and neck squamous cell carcinoma (HNSCC) is a prevalent and lethal malignancy, accounting for 95% of head and neck cancers. Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activating protein ZETA (14-3-3ζ) is central to various signalling pathways and is pivotal in tumour progression.MethodsCancerous and corresponding non-cancerous tissue samples were collected from five patients diagnosed with HNSCC. circRNA and mRNA expression profiles were analyzed using high-throughput sequencing techniques. Potential circRNA-microRNA (miRNA)-mRNA interactions were predicted using bioinformatics tools.ResultsThe study found that CircRNA_036186 regulates the expression of 14-3-3ζ in HNSCC through miR-193b-5p.DiscussionThese findings suggest that CircRNA_036186 has the potential to be a biomarker and therapeutic target for HNSCC and provide some theoretical basis for further research on the role of circRNA in HNSCC.
Global research trends in therapeutic drug monitoring of antimicrobials from 2000 to 2023: a bibliometric analysis
Authors: Li Hao , Jiang Manxue , Kong Lingti 
Published: December 2024

ObjectiveThe practice of therapeutic drug monitoring (TDM) is widely used for maximizing the clinical efficacy of antimicrobials. However, a systematic bibliometric analysis providing an overview of this field is lacking at present. The aim of the current study was to identify hotspots and trends in antimicrobial TDM, highlight collaborations and influences among countries, institutions, and journals, and assess the knowledge base for further development of clinical research.Research Design and MethodsArticles and reviews related to TDM of antimicrobials from the Web of Science Core Collection were collected. CiteSpace and VOSviewer, two visualization tools, were utilized to graphically assess the key elements within this domain, including mapping of countries and regions, institutions, keywords, and references associated with the field of antimicrobial TDM. Through this approach, we were able to successfully provide a comprehensive visual overview of the research landscape, highlighting the significant players and thematic trends in the literature.ResultsFrom 2000 to 2023, a total of 17,236 authors from 4,112 institutions in 112 countries/regions published 3,710 papers in 819 academic journals. The United States had the highest number of publications, with University of Queensland identified as the most active institution. The journal with the greatest number of publications was Therapeutic Drug Monitoring, whereas Antimicrobial Agents and Chemotherapy was the most co-cited journal. Current research focuses on pharmacokinetics, pharmacodynamics, vancomycin, posaconazole, invasive fungal infection and critically ill patients. Promising hotspots for future research include vancomycin, voriconazole, meropenem, isavuconazole, posaconazole, and teicoplanin. Moreover, vancomycin and critically ill patients remain a hot topic of future research.ConclusionUsing bibliometric and visualization methods, the research hotspots of antimicrobial drugs in TDM were analyzed. The continued increase in the number of annual publications demonstrates the vital significance of TDM for antimicrobials. Data from this study provide a valuable reference for future research trends in TDM of antimicrobial agents.
Glycyrol targets Pneumolysin (PLY) oligomerization to reduce Streptococcus pneumoniae toxicity
Authors: Li Yudi , Wu Hongji , Hu Yibo , Meng Haoji , Xu Yan 
Published: December 2024

Aim of the studyExploring the potential of glycyrol to reduce the invasiveness of Streptococcus pneumoniae (S. pneumoniae).Materials and MethodsCell experiments were performed using A549 alveolar epithelial cells and S. pneumoniae D39. Glycyrol was added to A549 cells mixed with or without Pneumolysin (PLY) to detect the effect of Glycyrol on PLY toxicity. Glycyrol was used to detect the effect on S. pneumoniae toxicity and PLY production. Mice was used to detect the anti-infectious ability of Glycyrol to regulate S. pneumoniae infection. Western blot and Molecular docking were used to detect how and where Glycyrol inhibits PLY toxicity.ResultsWe discovered that glycyrol, a main component of the widely recognized Chinese herbal medicine licorice, reduce the virulence of PLY in S. pneumoniae invasion; glycyrol achieves this effect by interacting with PLY through hydrogen bonding, van der Waals interactions, and solvation effects to reduce the pore-forming toxicity of PLY. Moreover, glycyrol did not affect the growth of S. pneumoniae or the production of PLY.ConclusionWe have actually discovered that Glycyrol, a major component of the widely known Chinese herbal medicine Glycyrrhiza uralensis Fisch., interacts with PLY through hydrogen bonds, Van der Waals and solvation to reduce the pore-forming toxicity of PLY and the toxicity of S. pneumoniae invasion, while not affecting the growth of S. pneumoniae and the production of PLY.
Diphenyl diselenide protects against diabetic kidney disease through modulating gut microbiota dysbiosis in streptozotocin-induced diabetic rats
Authors: Wang Xing , Long Dongmei , Peng Xingcan , Li Jiaxuan , Zhou Maoting , Wang Yu , Hu Xianghong 
Published: December 2024

IntroductionDiphenyl diselenide (DPDS) ameliorates nephropathy in streptozotocin (STZ)-induced type 1 diabetic rats by inhibiting oxidative stress and inflammatory reactions. However, it has not been clarified whether DPDS alleviates type 1 diabetic kidney disease (DKD) is related to the inhibition of extracellular matrix (ECM) production and the regulation of intestinal flora disorder.MethodsThe present study investigated the effects of DPDS on ECM generation in the kidney and intestinal microflora composition in feces. The rats were orally administered DPDS or metformin for eight weeks. Various indices were measured to assess the severity of renal injury. After euthanizing the rats, oxidative stress markers in serum and kidney were assessed using biochemical methods, and the expressions of ECM-related proteins in kidney were analyzed using Western blot. Additionally, 16S rRNA high-throughput sequencing was used to evaluate the diversity and composition of the intestinal flora in feces.ResultsThe results showed DPDS and metformin improved the DKD in STZ rats, as evidenced by decreased blood glucose, BUN, urine volume, urine microalbumin, urinary β2 microglobulin, and improvement of renal pathological morphology. Furthermore, DPDS intervention markedly reduced the protein expression of α-SMA, COI Ⅳ, FN, and vimentin in the kidneys. Besides, DPDS not only improved dyslipidemia in STZ diabetic rats, but also enhanced the activities of antioxidant enzymes, decreased the level of MDA in serum and kidney, and regulated the expression of proteins related to the Nrf2/Keap1 signaling pathway in the kidney. Moreover, we found that DPDS could selectively improve the relative abundance of probiotics as well as the diversity of flora, thus ameliorating the intestinal microbial composition of the STZ rats, significantly regulating the intestinal microbial homeostasis.DiscussionOverall, DPDS inhibited ECM production and improved renal pathological changes, which may be related to reducing oxidative stress damage in the kidney and improving intestinal flora imbalance, providing data support for the further development and application of DPDS in DKD.
Study on the changes of miRNAs and their target genes in regulating anthocyanin synthesis during purple discoloration of cauliflower curd under low temperature stress
Authors: Yao Xingwei , Zhang Qi , Chen Haidong , Ge Xianhong , Guo Yangdong , Chen Daozong 
Published: December 2024

IntroductionCauliflower is widely cultivated all over the world is attributed to its palatable flavor, high levels of anti-cancer compounds, and diverse array of nutrients. Exposure to extremely cold stress during production can result in a more frequent occurrence of purple discoloration in cauliflower curds. In response to cold stress, plants naturally produce anthocyanins to eliminate reactive oxygen species (ROS) generated as a defense mechanism.MethodsThis research involved conducting mRNA sequencing analysis on cauliflower curds both before and after exposure to cold stress treatment.ResultsIt was determined that the up-regulation of anthocyanin biosynthesis-related genes CHS, CHI, DFR, ANS, UGFT, PAP1/2, and MYBL2 occurred significantly in response to cold stress, resulting in a significant increase in total anthocyanin content. Subsequently, miRNA sequencing was employed to identify miRNAs in cauliflower curds, followed by differential expression analysis. The results showed that Bna-miR289 and Ath-miR157a may play a key role in regulating the accumulation of anthocyanin in cauliflower curds. Furthermore, we utilized degradome sequencing data to predict the target genes of the identified miRNAs, resulting in the identification of BolK_3g48940.1, BolK_9g11680.1, BolK_7g41780.1, BolK_3g68050.1, and BolK_3g729700.1 as targets. Subsequently, the expression patterns of the miRNAs and their target genes were validated using qRT-PCR, the results showed that Ath-miR157a and its target genes BolK_3g68050.1 and BolK_3g72970.1 may be the key to the purple of cauliflower curds under cold stress. DiscussionOur preliminary findings identified key miRNAs and their target genes that may be involved in regulating anthocyanin synthesis, thereby enhancing the cold tolerance of cauliflower through mRNA, miRNA, and degradome sequencing. Overall, our study sheds light on the activation of anthocyanin synthesis in flower curds under cold stress conditions as a mechanism to enhance resilience to adverse environmental conditions.
The manifestation and causes of public panic in the early stage of COVID-19 in China: a framework based on consciousness-attitude-behavior
Authors: Wei Changwei , Xu Jiaxi , Xu Zuying 
Published: December 2024

BackgroundThe onset of the COVID-19 pandemic brought about a stark and devastating impact on global scales, affecting countries and their citizens profoundly. The public’s lack of readiness for such an enigmatic and virulent threat led to widespread alarm, catalyzing a paradigm shift in both public conduct and governmental tactics. In the midst of this urgency, there was a notable lack of studies on the initial panic waves. Our study is designed to investigate the dynamics of public panic during the early stages of the pandemic, including its origins, and the public’s perceptions and behaviors.MethodsOur research, conducted through a questionnaire survey employing snowball sampling, gathered critical data on the public’s awareness, attitudes, and behaviors related to panic between February 23rd and March 25th, 2020.ResultsThe findings indicate a period of exceptionally intense and authentic public panic. This panic was a pervasive sentiment, manifesting in strong endorsements for rigorous epidemic control measures and heightened anxiety over virus-related information and family safety. The rapid spread of panic was also a notable characteristic.ConclusionThe public panic in response to COVID-19 was modulated by stringent prevention measures, with anxiety levels differing significantly based on occupation and health awareness. Notably, the rise of suspicious and distrustful actions was inextricably linked to an overwhelming sense of fear that gripped the public.
Childcare as a social determinant of access to healthcare: a scoping review
Authors: McArthur Megan , Tian Persephone , Kho Kimberly A. , Bhavan Kavita P. , Balasubramanian Bijal A. , Ganguly Anisha P. 
Published: December 2024

IntroductionAs health systems strive to screen for and address social determinants of health (SDOH), the role of access to childcare and barriers to healthcare posed by childcare needs remains underexplored. A gap exists in synthesizing existing evidence on the role of access to childcare as a SDOH.MethodsThis scoping review aimed to examine and analyze existing literature on the role of childcare needs as a social determinant of access to healthcare. We conducted a structured literature search across PubMed, Scopus, health policy fora, and professional healthcare societies to inclusively aggregate studies across interdisciplinary sources published between January 2000 and June 2023. Two independent reviewers reviewed results to determine inclusions and exclusions. Studies were coded into salient themes utilizing an iterative inductive approach.ResultsAmong 535 search results, 526 met criteria for eligibility screening. Among 526 eligible studies, 91 studies met inclusion criteria for analysis. Five key themes were identified through data analysis: (1) barriers posed by childcare needs to healthcare appointments, (2) the opportunity for alternative care delivery models to overcome childcare barriers, (3) the effect of childcare needs on participation in medical research, (4) the impact of the COVID-19 pandemic on childcare needs, and (5) the disproportionate burden of childcare experienced by vulnerable populations.DiscussionChildcare needs remain underexplored in existing research. Current evidence demonstrates the relevance of childcare needs as a barrier to healthcare access, however dedicated studies are lacking. Future research is needed to understand mechanisms of childcare barriers in access to healthcare and explore potential interventions.
Screening for Hypophosphatasia in Adult Patients at a Maximum Care Provider—Retrospective Analyses over Fifteen Years
Authors: Hennings Robert , Le Duc Diana , Bundalian Linnaeus , Tönjes Anke , Lemke Johannes R. , Thiery Joachim , Kratzsch Jürgen , Roth Andreas 
Published: December 2024

Background/Objectives: Hypophosphatasemia (HPE) may be temporary (tHPE) in the context of severe diseases, such as sepsis or trauma, or it may persist (pHPE), indicating an adult form of hypophosphatasia (HPP; OMIM 171760), a rare metabolic bone disorder caused by pathogenic nucleotide variants (PNVs) in the ALPL gene. The aim of this study was to analyze the role of auxiliary general biomarkers in verifying low alkaline phosphatase (ALP) serum activity level as an alert parameter for PNVs in the ALPL gene, which are indicative of HPP. In this retrospective analysis, we examined adult patients with an ALP serum activity level below 21 U/L. The cohort comprised 88 patients with temporary HPE (tHPE group) and 20 patients with persistent HPE who underwent re-examination. Genetic analysis performed on 12 pHPE patients identified PNV in the ALPL gene in 11 cases (ALPL group). Hemoglobin [HB], aspartate aminotransferase [AST], gamma-glutamyl transferase [GGT], calcium, phosphate, thyrotropin [TSH], albumin, total protein, and C-reactive protein [CRP] levels represented basic biomarkers. A comparative analysis between groups employed a Student’s t-test, and a Student’s t-test with bootstrap sampling (n = 10.000) was performed. Results: The mean HB, ALP, calcium, albumin, and total protein levels were lower in the tHPE group compared with the ALPL group (p < 0.01). AST and CRP were increased in the tHPE group (p < 0.01). The model showed an accuracy of 90% and an AUC of 0.94, which means that it can discern the two groups ~94% of the time. Conclusions: Basic biomarker evaluation effectively supports the interpretation of a decreased ALP serum activity level in the context of suspected HPP. In patients with laboratory HPE and biomarkers within reference, a PNV in the ALPL gene is highly suspected.
Geography, Antimicrobial Resistance, and Genomics of Salmonella enterica (Serotypes Newport and Anatum) from Meat in Mexico (2021–2023)
Authors: Reynoso Eduardo Canek , Delgado-Suárez Enrique Jesús , Hernández-Pérez Cindy Fabiola , Chavarin-Pineda Yaselda , Godoy-Lozano Elizabeth Ernestina , Fierros-Zárate Geny , Aguilar-Vera Omar Alejandro , Castillo-Ramírez Santiago , Gómez-Pedroso Luz del Carmen Sierra , Sánchez-Zamorano Luisa María 
Published: December 2024

Salmonella enterica non-typhoidal is a major contributor to diarrheal diseases, with over 2600 serovars identified across diverse environments. In Mexico, serovars Newport and Anatum have shown a marked increase, especially in foodborne disease, posing a public health problem. We conducted a cross-sectional study from 2021 to 2023 using active epidemiological surveillance to assess contamination in ground beef and pork at butcher shops nationwide. It involved isolation, phenotypic antimicrobial resistance, comparative genomics, spatial distribution, antimicrobial-resistance genes, and pangenome analysis. A total of 402 non-typhoidal S. enterica strains were isolated, including 59 Newport and 50 Anatum. After curating for redundancy, 45 Newport and 32 Anatum strains remained. We found that 75% of Newport strains exhibited multidrug resistance (MDR), compared to 25% of Anatum strains. Salmonella Newport also showed a broader distribution and stronger antibiotic-resistance capacity, particularly due to genes such as mphA and ramA. Our pangenome analysis showed a predominance of cell maintenance and survival-process genes in the accessory genome of both serotypes. Considering unique genes, Salmonella Anatum and Newport showed a notorious abundance of genes with functions related to replication, recombination, and repair. The substantial rise of Anatum and Newport strains in meat samples for human consumption presents an epidemiological alert, highlighting the critical need for stringent surveillance programs to mitigate human and ecosystem health risks.