bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histamine induces intracellular Ca2+ oscillations and nitric oxide release in endothelial cells from brain microvascular circulation
Authors: Berra‐Romani Roberto , Faris Pawan , Pellavio Giorgia , Orgiu Matteo , Negri Sharon , Forcaia Greta , Var‐‐‐gaz‐Guadarrama Verónica , Garcia‐Carrasco Mario , Botta Laura , Sancini Giulio , Laforenza Umberto , Moccia Francesco 
Published: July 2019

AbstractThe neuromodulator histamine is able to vasorelax in human cerebral, meningeal and temporal arteries via endothelial histamine 1 receptors (H1Rs) which result in the downstream production of nitric oxide (NO), the most powerful vasodilator transmitter in the brain. Although endothelial Ca
2+ signals drive histamine‐induced NO release throughout the peripheral circulation, the mechanism by which histamine evokes NO production in human cerebrovascular endothelial cells is still unknown. Herein, we exploited the human cerebral microvascular endothelial cell line, hCMEC/D3, to assess the role of intracellular Ca
2+ signaling in histamine‐induced NO release. To achieve this goal, hCMEC/D3 cells were loaded with the Ca
2+‐ and NO‐sensitive dyes, Fura‐2/AM and DAF‐FM/AM, respectively. Histamine elicited repetitive oscillations in intracellular Ca
2+ concentration in hCMEC/D3 cells throughout a concentration range spanning from 1 pM up to 300 μM. The oscillatory Ca
2+ response was suppressed by the inhibition of H
1Rs with pyrilamine, whereas H
1R was abundantly expressed at the protein level. We further found that histamine‐induced intracellular Ca
2+ oscillations were initiated by endogenous Ca
2+ mobilization through inositol‐1,4,5‐trisphosphate‐ and nicotinic acid dinucleotide phosphate‐sensitive channels and maintained over time by store‐operated Ca
2+ entry. In addition, histamine evoked robust NO release that was prevented by interfering with the accompanying intracellular Ca
2+ oscillations, thereby confirming that the endothelial NO synthase is recruited by Ca
2+ spikes also in hCMEC/D3 cells. These data provide the first evidence that histamine evokes NO production from human cerebrovascular endothelial cells through intracellular Ca
2+ oscillations, thereby shedding novel light on the mechanisms by which this neuromodulator controls cerebral blood flow.
Tobacco cigarette smoking exacerbates aortic calcification in an early stage of myocardial infarction in a female mouse model
Authors: Zalghout Sara , Kaplan Abdullah , Abidi Emna , El‐Achkar Ghewa A. , Nour‐Eldine Wared , Khalil Asmaa A. , Kobeissy Firas , Husari Ahmad , Habib Aida , Zouein Fouad A. , Hamade Eva 
Published: July 2019

AbstractDespite increased social awareness, marketing restraints, tobacco taxation, and available smoking cessation rehab programs, active and passive smoking remain a worldwide challenging epidemic and a key risk factor for cardiovascular diseases development. Although cardiovascular (CV) protection is more pronounced in women than in men due to estrogenic effects, tobacco cigarette smoking exposure seems to alter this protection by modulating estrogen actions via undefined mechanisms. Premenopausal cigarette smoking women are at higher risk of adverse CV effects than non‐smokers. In this study, we investigated the impact of cigarette smoking on early CV injury after myocardial infarction (MI) in non‐menopausal female mice. Aortic arch calcification, fibrosis, reactive oxygen species, and gene expression of inflammatory and calcification genes were exaggerated in mice exposed to cigarette smoke (CS). These findings suggest that aortic injury following MI, characterized by vascular smooth muscle cells transdifferentiation, calcification, inflammation, and collagen deposition but not cardiac dysfunction is exacerbated with CS exposure. The novel findings of this study highlight the importance of aortic injury on short and long‐term prognosis in CS‐exposed MI females. Linking those findings to estrogen alteration is probable and entails investigation.
Identification of a four‐gene metabolic signature predicting overall survival for hepatocellular carcinoma
Authors: Liu Gao‐Min , Xie Wen‐Xuan , Zhang Cai‐Yun , Xu Ji‐Wei 
Published: July 2019

AbstractWhile hundreds of consistently altered metabolic genes had been identified in hepatocellular carcinoma (HCC), the prognostic role of them remains to be further elucidated. Messenger RNA expression profiles and clinicopathological data were downloaded from The Cancer Genome Atlas—Liver Hepatocellular Carcinoma and GSE14520 data set from the Gene Expression Omnibus database. Univariate Cox regression analysis and lasso Cox regression model established a novel four‐gene metabolic signature (including acetyl‐CoA acetyltransferase 1, glutamic‐oxaloacetic transaminase 2, phosphatidylserine synthase 2, and uridine‐cytidine kinase 2) for HCC prognosis prediction. Patients in the high‐risk group shown significantly poorer survival than patients in the low‐risk group. The signature was significantly correlated with other negative prognostic factors such as higher α‐fetoprotein. The signature was found to be an independent prognostic factor for HCC survival. Nomogram including the signature shown some clinical net benefit for overall survival prediction. Furthermore, gene set enrichment analyses revealed several significantly enriched pathways, which might help explain the underlying mechanisms. Our study identified a novel robust four‐gene metabolic signature for HCC prognosis prediction. The signature might reflect the dysregulated metabolic microenvironment and provided potential biomarkers for metabolic therapy and treatment response prediction in HCC.
Predictors of invasive cancer of large laterally spreading colorectal tumors: A multicenter study in Japan
Authors: Kobayashi Kiyonori , Tanaka Shinji , Murakami Yoshitaka , Ishikawa Hideki , Sada Miwa , Oka Shiro , Saito Yutaka , Iishi Hiroyasu , Kudo Shin‐ei , Ikematsu Hiroaki , Igarashi Masahiro , Saitoh Yusuke , Inoue Yuji , Hisabe Takashi , Tsuruta Osamu , Sano Yasushi , Yamano Hiroo , Shimizu Seiji , Yahagi Naohisa , Matsuda Keiji , Nakamura Hisashi , Fujii Takahiro , Sugihara Kenichi ,   the Colorectal Endoscopic Resection Standardization Implementation Working Group of the Japanese Society for Cancer of the Colon and Rectum
Published: July 2019

Background and AimAlthough colorectal laterally spreading tumors (LSTs) can be classified into four subtypes, the histopathological characteristics are known to differ among these subtypes. We therefore performed a logistic regression analysis to determine whether the risk of pathological T1 cancer of large colorectal LSTs can be predicted based on factors such as endoscopic findings in a large group of patients enrolled in a multicenter study in Japan.MethodsIn the main study, we assessed 1236 colorectal adenomas or early cancers that were classified as LSTs measuring 20 mm or more in diameter and treated endoscopically. Logistic regression analysis was performed to determine whether factors such as the subtype of LST could be used to predict the risk of pathological T1 cancer. A validation study of 356 large colorectal LSTs was conducted to confirm the validity of the results obtained in the main study.ResultsThe locations and tumor diameter of the LSTs in the main study were found to differ significantly according to the LST subclassification (P < 0.001). The frequency of pathological T1 cancers was the highest at 36% of LST nongranular pseudodepressed type, followed by 14% of LST nongranular flat‐elevated type, 11% of LST granular nodular mixed type, and 3% of LST granular homogenous type lesions. The risk of pathological T1 cancer was significantly associated with LST subclassification and tumor diameter. The area under the curve (AUC) was high (0.743). In the validation study, the AUC was 0.573.ConclusionsIn patients with large colorectal LSTs resected endoscopically, the risk of pathological T1 cancer can be predicted on the basis of the LST subclassification and tumor diameter.
Future liver remnant portal or hepatic vein reconstruction at stage I of ALPPS—How far can we push the boundaries?
Authors: Schlegel Andrea , Sakuraoka Yuhki , Kalisvaart Marit , Coldham Chris , Isaac John , Muiesan Paolo 
Published: July 2019

AbstractPreservation of the future liver remnant (FLR) vascular integrity has always been considered crucial to achieving successful liver growths after major hepatectomies. Most surgeons appeared therefore reluctant to combine stage I of associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) with vascular reconstructions. Here we describe a case series, where we combine parenchymal transection and venous in‐ or outflow reconstruction of the FLR at stage I of ALPPS. In addition, the cold flush of the FLR or delayed portal vein embolization is applied in selected cases.
Summary of hands‐on supermicrosurgery course and live surgeries at 8th world symposium for lymphedema surgery
Authors: Pappalardo Marco , Chang David W. , Masia Jaume , Koshima Isao , Cheng Ming‐Huei 
Published: July 2019

AbstractThe hands‐on supermicrosurgery course provided participants a valuable learning experience of in‐depth practices of supermicrosurgical skills with experts. Seven live surgeries were successfully demonstrated at 8th World Symposium for Lymphedema Surgery. Variable donor sites for vascularized lymph node transfer were the submental, supraclavicular, groin, and omental; while the recipient sites included the wrist and axilla in upper limb; and popliteal and groin in the lower limb. The therapeutic and preventive lymphovenous anastomosis was also satisfactorily performed.
Separation of antipyretic analgesics by open tubular capillary electrochromatography with homopolymer coatings
Authors: Liu Lili , Qiao Juan , Zhang Hongyi , Qi Li 
Published: July 2019

AbstractThis study developed an open‐tubular capillary electrochromatography protocol for the analysis of antipyretic analgesic drugs, which used a multifunctional homopolymer as coating. A controlled/living radical polymerization strategy was adopted to obtain poly(N‐acryloxysuccinimide) with a tunable chain‐length. The homopolymer coating enhanced the separation performance by contributing to the hydrophobic and hydrogen‐bonding interactions between the analytes and the homopolymer. The effect of polymer chain‐length and buffer pH and concentration on the separation efficiency was evaluated. In this approach, baseline separation of the three test drugs was achieved within 15 min. The repeatability of the prepared homopolymer coating was investigated, with the relative standard deviations < 2.88% observed in intra‐ and interday runs. Good linearity in the 5–800 µM range (R2 ≥ 0.998) demonstrates that accurate quantitative analysis of real samples was achieved. Moreover, the proposed assay was used to quantify the three drugs (aminopyrine, 4‐aminoantipyrine, and phenacetin) in urine samples, achieving recovery rates between 92.1 and 108.7%. This promising methodology may be used for the analysis of drugs in real bio‐samples and for the development of unique homopolymer coatings for open‐tubular capillary electrochromatography systems.
Seagrass restoration reestablishes the coastal nitrogen filter through enhanced burial
Authors: Aoki Lillian R. , McGlathery Karen J. , Oreska Matthew P. J. 
Published: July 2019

AbstractSeagrass meadows perform an important ecological function as filters for incoming nutrients from surrounding watersheds, especially nitrogen (N). By enhancing N removal processes, including N burial in sediments and denitrification, seagrass meadows improve water quality. With accelerating losses of seagrass meadows worldwide, seagrass restoration plays a key role in reestablishing these coastal ecosystem functions. However, few measurements exist of N burial rates in temperate seagrass meadows and none have been published for restored meadows. In this study, we measured N burial rates in a large (6.9 km2) restored eelgrass (Zostera marina) meadow and compared N removal through burial to previous measurements of removal via denitrification. We also compared N removal to inputs from external loading and fixation and to N assimilation in seagrass biomass. We found that, in this meadow, burial was the dominant process of N removal; the burial rate of 3.52 g N m−2 yr−1 was comparable to rates in natural meadows within 10 yr after seeding and was more than 20× the rate in adjacent bare sediments (0.17 g N m−2 yr−1). We also found that the high rates of N assimilation (2.62 g N m−2 yr−1) created a substantial though temporary sink for nitrogen during the growing season. Our results highlight how seagrass meadows mediate N cycling through high rates of burial, which to date has been understudied in the literature. The successful return of the N filter function after restoration, shown here for the first time, can motivate continued efforts for seagrass restoration and conservation.
Ocean acidification alters meiobenthic assemblage composition and organic matter degradation rates in seagrass sediments
Authors: Ravaglioli Chiara , Lardicci Claudio , Pusceddu Antonio , Arpe Eleonora , Bianchelli Silvia , Buschi Emanuela , Bulleri Fabio 
Published: July 2019

AbstractSeagrass meadows are an important organic matter (OM) reservoir but, are currently being lost due to global and regional stressors. Yet, there is limited research investigating the cumulative impacts of anthropogenic stressors on the structure and functioning of seagrass benthic assemblages, key drivers of OM mineralization and burial. Here, using a 16‐month field experiment, we assessed how meiobenthic assemblages and extracellular enzymatic activities (as a proxy of OM degradation) in Posidonia oceanica sediments responded to ocean acidification (OA) and nutrient loadings, at CO2 vents. P. oceanica meadows were exposed to three nutrient levels (control, moderate, and high) at both ambient and low pH sites. OA altered meiobenthic assemblage structure, resulting in increased abundance of annelids and crustaceans, along with a decline in foraminifera. In addition, low pH enhanced OM degradation rates in seagrass sediments by enhancing extracellular enzymatic activities, potentially decreasing the sediment carbon storage capacity of seagrasses. Nutrient enrichment had no effect on the response variables analyzed, suggesting that, under nutrient concentration unlikely to cause N or P imitation, a moderate increase of dissolved nutrients in the water column had limited influence on meiobenthic assemblages. These findings show that OA can significantly alter meiobenthic assemblage structure and enhance OM degradation rates in seagrass sediments. As meiofauna are ubiquitous key actors in the functioning of benthic ecosystems, we postulated that OA, altering the structure of meiobenthic assemblages and OM degradation, could affect organic carbon sequestration over large spatial scales.
Non‐Ablative Fractional 1,540‐nm Er:Glass Laser in the Treatment of Atrophic Cutaneous Leishmaniasis Scars
Authors: Taheri Ahmad Reza , Mashayekhi‐Goyonlo Vahid , Salehi Maryam , Mohammadzadeh Hamideh 
Published: July 2019

ObjectivesTo evaluate the efficacy of nonablative fractional 1,540 nm laser to treat the atrophic scars caused by the cutaneous leishmaniasis (CL).MethodsThis clinical trial with a pre‐ and a posttreatment measurement was conducted on patients with atrophic CL scars. The lesions were treated with nonablative fractional 1,540 nm laser. We evaluated the patients initially and then monthly, before each treatment session. The final follow‐up was done 6 months after the end of study for all patients. Patient assessment was performed by two physicians using the modified Manchester Scar Scale (MSS) as well as the interpretation of captured digital photographs. Moreover, the patients performed a self‐assessment by filling in a researcher‐made questionnaire. The data were statistically analyzed by SPSS software. P < 0.05 was considered statistically significant.ResultsThirty patients with 37 skin lesions participated in the study. The pairwise comparison demonstrated a statistically significant difference between the modified MSS parameters (P < 0.05); however, no significant difference was observed between the modified MSS of the third and fourth (P = 0.82) as well as fourth and fifth (P = 0.636) sessions. The lesions improvement was significant based on the physician's evaluation (P < 0.001). Furthermore, patients’ level of satisfaction was significantly increased in all six follow‐ups (P < 0.001). No persistent complication was found.ConclusionsNonablative fractional 1,540 nm laser is an effective and safe therapeutic choice for atrophic CL, even in darker skins. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.
High‐temperature corrosion behavior of some post‐plasma‐spraying‐gas‐nitrided metallic coatings on a Fe‐based superalloy
Authors: Chawla Vikas , Sidhu Buta S. , Rani Amita , Handa Amit 
Published: July 2019

AbstractThe objective of the present study is to propose a cost‐effective process for modifying commercially available coatings by gas nitriding using commonly available equipment and starting materials. Al–Cr and Ti–Al metallic coatings were deposited on Superfer 800H (Fe‐based superalloy) using a plasma spray process. Then the gas nitriding of the coatings was done in the lab and the parameters were optimized after conducting several trials on plasma‐sprayed‐coated specimens. Characterization and high‐temperature corrosion behavior of coatings after exposure to air and molten salt at 900°C were studied under cyclic conditions. Techniques like XRD, SEM/EDX, and X‐ray mapping analysis were used for the characterization of the coatings and analysis of the oxide scale. Both the coatings successfully protected the substrate and were effective in decreasing the corrosion rate when subjected to cyclic oxidation (Type‐I hot corrosion) at 900°C for 50 cycles in air and molten salt (a salt mixture of Na2SO4–60%V2O5 dissolved in distilled water). Based on the findings of the present study, the coatings under study are recommended for tapplications to super‐heater and reheater tubes of boilers and all those surfaces that face fireside corrosion, such as fluidized beds, industrial waste incinerators, internal combustion engines, gas turbines or steam turbines, to provide protection against degradation in these environments. The cost of the product/process is approximately Rs. 0.62 per mm2 in case of Al–Cr coating and Rs. 1.86 per mm2 in case of Ti–Al coating.
Electrochemical corrosion behavior of micrometer‐sized porous Ti3SiC2 compounds in NaCl solution
Authors: Jiang Yao , He Yuehui 
Published: July 2019

The electrochemical corrosion behavior of reactively synthesized porous Ti3SiC
2 in 1 M NaCl solution was investigated compared with that of porous TiAl. Porous Ti
3SiC
2 and TiAl have high‐purity single phase characteristics with open porosities of 48% and 41% and maximum apertures of 6.9 and 16.3 µm, respectively, which show positive direction shift behavior of open circuit potential at the early immersion stage with stable potential ranges of 0.25~0.27 and −0.36~−0.38 V (vs. saturated calomel), respectively. The electrochemical corrosion procedures of the two porous compounds exhibit three‐stage characteristics of activation, passivation, and breakdown. Porous Ti
3SiC
2 has a much larger ohmic resistance and Warburg coefficient than TiAl. The passivation films of the two porous compounds consist of an inner layer with a larger resistance and a porous outer layer. Porous Ti
3SiC
2 has a significantly larger resistance and relatively smaller capacitance for the inner passivation film, indicating better corrosion resistance stability.
Relevance of the Polymeric Prodrug and Its Drug Loading Efficiency: Comparison between Computer Simulation and Experiment
Authors: Luo Xueli , Wang Shenchun , Xu Sishi , Lang Meidong 
Published: July 2019

AbstractIn order to explore the relationship between drug grafting ratio of polymeric prodrug and its drug loading efficiency, molecular dynamics simulation is utilized to calculate the compatibility of polymeric prodrug with 5‐aminolevulinic acid methyl ester (m‐ALA) and H2O first. Then, dissipative particle dynamics simulation is used to observe the morphologies of drug‐loaded micelles. Then, designed polymeric prodrugs are synthesized, drug‐loaded micelles and blank micelles are prepared by dialysis method, and the drug loading content (DLC) and encapsulation efficiency are measured. The computer simulation shows that polymer–drug compatibility gets better as the m‐ALA grafting ratio increases from 0% to 100%. Experimental results show that the DLC of micelle changes from 4.05% (0% grafting ratio) to 8.99% (100% grafting ratio). Through comparison, the experimental results are proven to be consistent with the computer simulation results, revealing that the drug loading efficiency of polymer micelles could be improved by grafting drugs.
The roles of transmembrane 6 superfamily member 2 rs58542926 polymorphism in chronic liver disease: A meta‐analysis of 24,147 subjects
Authors: Chen Xinpei , Zhou Pengcheng , De Luo , Li Bo , Su Song 
Published: July 2019

AbstractBackgroundSome genetic association studies tried to investigate potential associations of transmembrane 6 superfamily member 2 (TM6SF2) polymorphisms with chronic liver disease. However, the results of these studies were not consistent. Thus, we performed the present meta‐analysis to explore associations between TM6SF2 polymorphisms and chronic liver disease in a larger pooled population.MethodsSystematic literature research of PubMed, Web of Science, Embase, and CNKI was performed to identify eligible studies for pooled analyses. I2 statistics were employed to assess between‐study heterogeneities. If I2 was greater than 50%, random‐effect models (REMs) would be used to pool the data. Otherwise, fixed‐effect models (FEMs) would be applied for synthetic analyses.ResultsTotally 28 studies were included for analyses (13,137 cases and 11,010 controls). The pooled analyses showed that rs58542926 polymorphism was significantly associated with chronic liver disease in overall population (dominant model: p < 0.0001, OR = 0.70, 95% CI = 0.64–0.76, I2 = 47%; recessive model: p < 0.0001, OR = 2.94, 95% CI = 2.05–4.20, I2 = 0%; over‐dominant model: p < 0.0001, OR = 1.34, 95% CI = 1.23–1.47, I2 = 0%; allele model: p < 0.0001, OR = 0.68, 95% CI = 0.63–0.73, I2 = 47%), and these significant findings were further confirmed in both Asians and Caucasians. Stratified analyses by type of disease revealed similar positive results in hepatocellular carcinoma (HCC), cirrhosis, alcoholic liver disease (ALD) and NAFLD (Nonalcoholic fatty liver disease), but not in chronic hepatitis B infection (CHB) and chronic hepatitis C infection (CHC).ConclusionsThese results suggested that TM6SF2 rs58542926 could be used to identify individuals at higher susceptibility to chronic liver disease, especially for HCC, cirrhosis, ALD, and NAFLD.