bluuebunny/crossref_metadata_2025_split huggingface dataset:
Doveweed (Murdannia nudiflora) Response to Environmental Resource Availability and Cultural Practices
Authors: Atkinson Jeffrey L. , McCarty Lambert B. , Yelverton Fred , McElroy Scott , Bridges William C. 
Published: March 2019

AbstractSusceptibility of a system to colonization by a weed is in part a function of environmental resource availability. Doveweed [Murdannia nudiflora(L.) Brenan] can establish in a variety of environments; however, it is found mostly in wet or low-lying areas with reduced interspecies competition. Four studies evaluated the effect of mowing height, interspecies competition, and nitrogen, light, and soil moisture availability onM. nudifloraestablishment and growth. A field study evaluated the effect of mowing height onM. nudifloraestablishment. In comparison with unmowed plots, mowing at 2 and 4 cm reduced spread 46% and 30%, respectively, at 9 wk after planting. Effect of mowing height and nitrogen fertilization on ‘Tifway’ bermudagrass (Cynodon dactylonBurtt-Davy×C. transvaalensisL. Pers.) andM. nudiflorainterspecies competition was evaluated in a greenhouse trial.Murdannia nudifloracoverage was 62% greater in flats maintained at 2.6 cm than flats maintained at 1.3 cm. Supplemental application of 49 kg N ha−1mo−1increasedM. nudifloracoverage 75% in comparison with 24.5 kg N ha−1mo−1. A difference inM. nudifloracoverage could not be detected between flats receiving 0 and 24.5 kg N ha−1mo−1, suggesting moderate nitrogen fertilization does not encourageM. nudifloracolonization. Effect of light availability onM. nudifloragrowth and development was evaluated in a greenhouse study. Growth in a 30%, 50%, or 70% reduced light environment (RLE) did not affect shoot growth on a dry weight basis in comparison with plants grown under full irradiance; however, internode length was 28% longer in a 30% RLE and 39% longer in a 50% and 70% RLE. Effect of soil moisture onM. nudifloragrowth and development was evaluated in a greenhouse study. Plants maintained at 50%, 75%, and 100% field capacity (FC) increased biomass>200% compared with plants maintained at 12.5% or 25% FC.
Grandiose and Vulnerable Narcissism in Relation to Life Satisfaction, Self-Esteem, and Self-Construal
Authors: Rohmann Elke , Hanke Stephanie , Bierhoff Hans-Werner 
Published: March 2019

Abstract. A growing body of research suggests the viability of the distinction between grandiose and vulnerable narcissism. Each subtype of narcissism reveals distinct associations with life satisfaction, self-esteem, and self-construal. The goal of the present study ( N = 253) is to apply this distinction to replicate the results of previous studies with respect to life satisfaction and self-esteem and to extend the perspective by focusing on several components of self-construal: independent and interdependent (cf. Singelis, 1994 ). In addition, interdependent self-construal is either relational-interdependent or collective-interdependent (cf. Cross, Hardin, & Gercek-Swing, 2011 ). Specifically, four hypotheses are examined which have in common the assumption that grandiose and vulnerable narcissism diverge systematically in their implications for life satisfaction, self-esteem, and self-construal. Grandiose narcissism is expected to correlate positively with life satisfaction, self-esteem, independent self-construal, and collective-interdependent self-construal. In contrast, vulnerable narcissism is assumed to correlate negatively with self-esteem and life satisfaction and positively with interdependent and relational self-construal. The results, which confirm these hypotheses, underscore the necessity to differentiate between grandiose and vulnerable narcissism. Remarkably, both subtypes of narcissism are related to distinct dimensions of interdependent self-construal. Whereas grandiose narcissism is anchored in collective interdependence, vulnerable narcissism is embedded in relational interdependence.
Assessing the Skill of the Improved Treatment of Riverine Freshwater in the Community Earth System Model (CESM) Relative to a New Salinity Climatology
Authors: Sun Qiang , Whitney Michael M. , Bryan Frank O. , Tseng Yu‐heng 
Published: March 2019

AbstractRecent studies have explored the sensitivity of global ocean model simulations to the treatment of riverine freshwater and the representation of estuarine processes via an estuary box model applied within Community Earth System Model (CESM). This study builds on these efforts by assessing the model skill score relative to a new salinity climatology. The new climatology averages the original observational data of the World Ocean Database directly onto the CESM ocean component tracer grid cells without spatial interpolation, smoothing, or other gap‐filling techniques to mitigate coastal ocean salinity bias present in the World Ocean Atlas. The mean square error for coastal upper ocean salinity relative to climatology is reduced by up to 14%, and the mean square error of near‐surface salinity stratification is reduced by up to 28% near major river mouths in the simulations with improved treatments of river runoff. The improvement in upper ocean bulk salinity is attributed primarily to focusing runoff as point sources thereby avoiding the artificial horizontal spreading of the control run and to applying a locally varying instead of a global constant reference salinity for riverine virtual salt fluxes. The improvements in near‐surface salinity stratification are primarily attributed to adding parameterized estuarine mixing with the estuary box model. Salinity and salinity stratification skill improvements are achieved not just near large rivers but also along the global coast and skill improvements extend far offshore. Despite these improvements, many other sources of model‐climatology mismatch in coastal salinity and stratification remain and merit further attention.
Machine learning metrology of cell confinement in melt electrowritten three-dimensional biomaterial substrates
Authors: Tourlomousis Filippos , Jia Chao , Karydis Thrasyvoulos , Mershin Andreas , Wang Hongjun , Kalyon Dilhan M. , Chang Robert C. 
Published: March 2019

AbstractTuning cell shape by altering the biophysical properties of biomaterial substrates on which cells operate would provide a potential shape-driven pathway to control cell phenotype. However, there is an unexplored dimensional scale window of three-dimensional (3D) substrates with precisely tunable porous microarchitectures and geometrical feature sizes at the cell’s operating length scales (10–100 μm). This paper demonstrates the fabrication of such high-fidelity fibrous substrates using a melt electrowriting (MEW) technique. This advanced manufacturing approach is biologically qualified with a metrology framework that models and classifies cell confinement states under various substrate dimensionalities and architectures. Using fibroblasts as a model cell system, the mechanosensing response of adherent cells is investigated as a function of variable substrate dimensionality (2D vs. 3D) and porous microarchitecture (randomly oriented, “non-woven” vs. precision-stacked, “woven”). Single-cell confinement states are modeled using confocal fluorescence microscopy in conjunction with an automated single-cell bioimage data analysis workflow that extracts quantitative metrics of the whole cell and sub-cellular focal adhesion protein features measured. The extracted multidimensional dataset is employed to train a machine learning algorithm to classify cell shape phenotypes. The results show that cells assume distinct confinement states that are enforced by the prescribed substrate dimensionalities and porous microarchitectures with the woven MEW substrates promoting the highest cell shape homogeneity compared to non-woven fibrous substrates. The technology platform established here constitutes a significant step towards the development of integrated additive manufacturing—metrology platforms for a wide range of applications including fundamental mechanobiology studies and 3D bioprinting of tissue constructs to yield specific biological designs qualified at the single-cell level.
Enhancing folic acid metabolism suppresses defects associated with loss of Drosophila mitofusin
Authors: Garrido-Maraver Juan , Celardo Ivana , Costa Ana C. , Lehmann Susann , Loh Samantha H. Y. , Martins L. Miguel 
Published: March 2019

AbstractMutations in the mitochondrial GTPase mitofusin 2 (MFN2) cause Charcot-Marie-Tooth disease type 2 (CMT2A), a form of peripheral neuropathy that compromises axonal function. Mitofusins promote mitochondrial fusion and regulate mitochondrial dynamics. They are also reported to be involved in forming contacts between mitochondria and the endoplasmic reticulum. The fruit fly, Drosophila melanogaster, is a powerful tool to model human neurodegenerative diseases, including CMT2A. Here, we have downregulated the expression of the Drosophila mitofusin (dMfn RNAi) in adult flies and showed that this activates mitochondrial retrograde signalling and is associated with an upregulation of genes involved in folic acid (FA) metabolism. Additionally, we demonstrated that pharmacological and genetic interventions designed to increase the FA metabolism pathway suppresses the phenotype of the dMfn RNAi flies. We conclude that strategies to increase FA metabolism may ameliorate diseases, such as peripheral neuropathies, that are associated with loss of mitochondrial function. A video abstract for this article is available at https://youtu.be/fs1G-QRo6xI.
Cervical cancer subtypes harbouring integrated and/or episomal HPV16 portray distinct molecular phenotypes based on transcriptome profiling of mRNAs and miRNAs
Authors: Mandal Paramita , Saha Sweta Sharma , Sen Shrinka , Bhattacharya Amrapali , Bhattacharya Nitai P. , Bucha Sudha , Sinha Mithun , Chowdhury Rahul Roy , Mondal Nidhu Ranjan , Chakravarty Biman , Chatterjee Tanmay , Roy Sudipta , Chattapadhyay Ansuman , Sengupta Sharmila 
Published: March 2019

AbstractHeterogeneity in cervical cancers (CaCx) in terms of HPV16 physical status prompted us to investigate the mRNA and miRNA signatures among the different categories of CaCx samples. We performed microarray-based mRNA expression profiling and quantitative real-time PCR-based expression analysis of some prioritised miRNAs implicated in cancer-related pathways among various categories of cervical samples. Such samples included HPV16-positive CaCx cases that harboured either purely integrated HPV16 genomes (integrated) and those that harboured episomal viral genomes, either pure or concomitant with integrated viral genomes (episomal), which were compared with normal cervical samples that were either HPV negative or positive for HPV16. The mRNA expression profile differed characteristically between integrated and episomal CaCx cases for enriched biological pathways. miRNA expression profiles also differed among CaCx cases compared with controls (upregulation—miR-21, miR-16, miR-205, miR-323; downregulation—miR-143, miR-196b, miR-203, miR-34a; progressive upregulation—miR-21 and progressive downregulation—miR-143, miR-34a, miR-196b and miR-203) in the order of HPV-negative controls, HPV16-positive non-malignant samples and HPV16-positive CaCx cases. miR-200a was upregulated in HPV16-positive cervical tissues irrespective of histopathological status. Expression of majority of the predicted target genes was negatively correlated with their corresponding miRNAs, irrespective of the CaCx subtypes. E7 mRNA expression correlated positively with miR-323 expression among episomal cases and miR-203, among integrated cases. miR-181c expression was downregulated only among the episomal CaCx cases and negatively correlated with protein coding transcript of the proliferative target gene, CKS1B of the significantly enriched “G2/M DNA Damage Checkpoint Regulation” pathway among CaCx cases. Thus, the two CaCx subtypes are distinct entities at the molecular level, which could be differentially targeted for therapy. In fact, availability of a small molecule inhibitor of CKS1B, suggests that drugging CKS1B could be a potential avenue of treating the large majority of CaCx cases harbouring episomal HPV16.
Discovery of HB-EGF binding peptides and their functional characterization in ovarian cancer cell lines
Authors: Shen Yanting , Ruan Lingling , Lian Caixia , Li Ruyan , Tu Zhigang , Liu Hanqing 
Published: March 2019

AbstractOvarian cancer is one of the most frequent causes of cancer death among all gynecologic cancers. Though standard therapy often results in temporary clinical remission, most patients suffer from recurrence and metastasis of ovarian cancer, which highlights the need for developing new therapeutic agents targeting specific molecules. Previous studies have demonstrated that the native ligand of epidermal growth factor receptor (EGFR) and ErbB4, heparin-binding EGF-like growth factor (HB-EGF), plays a critical role in the progression of ovarian cancer and is associated with prognosis of ovarian cancer. In the current study, we tried to develop a peptide-based treatment for ovarian cancer by targeting HB-EGF. After the functions of HB-EGF in promoting migration and invasion of SKOV3 and HO-8910 cells were confirmed, phage display was used to discover peptides binding to HB-EGF. Two peptides, no. 7 and no. 29 were found mildly binding to HB-EGF. Then the effects of these peptides on HB-EGF functions were examined and both peptides no. 7 and no. 29 were found indeed inhibiting the functions of HB-EGF in promoting migration and invasion of SKOV3 and HO-8910 cells in vitro. Further mechanism investigation showed that peptides no. 7 and no. 29 inhibited HB-EGF-promoted cell migration and invasion through attenuating activation of the EGFR signaling pathway manifested by decreased p-Erk1/2 and Snail levels. More importantly, peptides no. 7 and no. 29 showed strong activities in inhibiting migration of SKOV3 cells in vivo. These results provide a proof of concept method for developing novel peptide drugs to combat ovarian cancer through interfering with HB-EGF mediated signaling pathways.
Bone marrow central memory and memory stem T-cell exhaustion in AML patients relapsing after HSCT
Authors: Noviello Maddalena , Manfredi Francesco , Ruggiero Eliana , Perini Tommaso , Oliveira Giacomo , Cortesi Filippo , De Simone Pantaleo , Toffalori Cristina , Gambacorta Valentina , Greco Raffaella , Peccatori Jacopo , Casucci Monica , Casorati Giulia , Dellabona Paolo , Onozawa Masahiro , Teshima Takanori , Griffioen Marieke , Halkes Constantijn J. M. , Falkenburg J. H. F. , Stölzel Friedrich , Altmann Heidi , Bornhäuser Martin , Waterhouse Miguel , Zeiser Robert , Finke Jürgen , Cieri Nicoletta , Bondanza Attilio , Vago Luca , Ciceri Fabio , Bonini Chiara 
Published: March 2019

AbstractThe major cause of death after allogeneic Hematopoietic Stem Cell Transplantation (HSCT) for acute myeloid leukemia (AML) is disease relapse. We investigated the expression of Inhibitory Receptors (IR; PD-1/CTLA-4/TIM-3/LAG-3/2B4/KLRG1/GITR) on T cells infiltrating the bone marrow (BM) of 32 AML patients relapsing (median 251 days) or maintaining complete remission (CR; median 1 year) after HSCT. A higher proportion of early-differentiated Memory Stem (TSCM) and Central Memory BM-T cells express multiple IR in relapsing patients than in CR patients. Exhausted BM-T cells at relapse display a restricted TCR repertoire, impaired effector functions and leukemia-reactive specificities. In 57 patients, early detection of severely exhausted (PD-1+Eomes+T-bet−) BM-TSCM predicts relapse. Accordingly, leukemia-specific T cells in patients prone to relapse display exhaustion markers, absent in patients maintaining long-term CR. These results highlight a wide, though reversible, immunological dysfunction in the BM of AML patients relapsing after HSCT and suggest new therapeutic opportunities for the disease.
A stem group echinoderm from the basal Cambrian of China and the origins of Ambulacraria
Authors: Topper Timothy P. , Guo Junfeng , Clausen Sébastien , Skovsted Christian B. , Zhang Zhifei 
Published: March 2019

AbstractDeuterostomes are a morphologically disparate clade, encompassing the chordates (including vertebrates), the hemichordates (the vermiform enteropneusts and the colonial tube-dwelling pterobranchs) and the echinoderms (including starfish). Although deuterostomes are considered monophyletic, the inter-relationships between the three clades remain highly contentious. Here we report,Yanjiahella biscarpa, a bilaterally symmetrical, solitary metazoan from the early Cambrian (Fortunian) of China with a characteristic echinoderm-like plated theca, a muscular stalk reminiscent of the hemichordates and a pair of feeding appendages. Our phylogenetic analysis indicates thatY.biscarpais a stem-echinoderm and not only is this species the oldest and most basal echinoderm, but it also predates all known hemichordates, and is among the earliest deuterostomes. This taxon confirms that echinoderms acquired plating before pentaradial symmetry and that their history is rooted in bilateral forms.Yanjiahella biscarpashares morphological similarities with both enteropneusts and echinoderms, indicating that the enteropneust body plan is ancestral within hemichordates.
Hemozoin-catalyzed precipitation polymerization as an assay for malaria diagnosis
Authors: Rifaie-Graham Omar , Pollard Jonas , Raccio Samuel , Balog Sandor , Rusch Sebastian , Hernández-Castañeda María Andrea , Mantel Pierre-Yves , Beck Hans-Peter , Bruns Nico 
Published: March 2019

AbstractMethods to diagnose malaria are of paramount interest to eradicate the disease. Current methods have severe limitations, as they are either costly or not sensitive enough to detect low levels of parasitemia. Here we report an ultrasensitive, yet low-resource chemical assay for the detection and quantification of hemozoin, a biomarker of all Plasmodium species. Solubilized hemozoin catalyzes the atom transfer radical polymerization of N-isopropylacrylamide above the lower critical solution temperature of poly(N-isopropylacrylamide). The solution becomes turbid, which can be observed by naked eye and quantified by UV-visible spectroscopy. The rate of turbidity increase is proportional to the concentration of hemozoin, with a detection limit of 0.85 ng mL−1. Malaria parasites in human blood can be detected down to 10 infected red blood cells μL−1. The assay could potentially be applied as a point-of-care test. The signal-amplification of an analyte by biocatalytic precipitation polymerization represents a powerful approach in biosensing.
Tuning Pt-CeO2 interactions by high-temperature vapor-phase synthesis for improved reducibility of lattice oxygen
Authors: Pereira-Hernández Xavier Isidro , DeLaRiva Andrew , Muravev Valery , Kunwar Deepak , Xiong Haifeng , Sudduth Berlin , Engelhard Mark , Kovarik Libor , Hensen Emiel J. M. , Wang Yong , Datye Abhaya K. 
Published: March 2019

AbstractIn this work, we compare the CO oxidation performance of Pt single atom catalysts (SACs) prepared via two methods: (1) conventional wet chemical synthesis (strong electrostatic adsorption–SEA) with calcination at 350 °C in air; and (2) high temperature vapor phase synthesis (atom trapping–AT) with calcination in air at 800 °C leading to ionic Pt being trapped on the CeO2 in a thermally stable form. As-synthesized, both SACs are inactive for low temperature (<150 °C) CO oxidation. After treatment in CO at 275 °C, both catalysts show enhanced reactivity. Despite similar Pt metal particle size, the AT catalyst is significantly more active, with onset of CO oxidation near room temperature. A combination of near-ambient pressure X-ray photoelectron spectroscopy (NAP-XPS) and CO temperature-programmed reduction (CO-TPR) shows that the high reactivity at low temperatures can be related to the improved reducibility of lattice oxygen on the CeO2 support.
A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane
Authors: Gómez-Santos Nuria , Glatter Timo , Koebnik Ralf , Świątek-Połatyńska Magdalena Anna , Søgaard-Andersen Lotte 
Published: March 2019

AbstractTonB-dependent transporters (TBDTs) are ubiquitous outer membrane β-barrel proteins that import nutrients and bacteriocins across the outer membrane in a proton motive force-dependent manner, by directly connecting to the ExbB/ExbD/TonB system in the inner membrane. Here, we show that the TBDT Oar in Myxococcus xanthus is required for secretion of a protein, protease PopC, to the extracellular milieu. PopC accumulates in the periplasm before secretion across the outer membrane, and the proton motive force has a role in secretion to the extracellular milieu. Reconstitution experiments in Escherichia coli demonstrate that secretion of PopC across the outer membrane not only depends on Oar but also on the ExbB/ExbD/TonB system. Our results indicate that TBDTs and the ExbB/ExbD/TonB system may have roles not only in import processes but also in secretion of proteins.
Clinical significance of the internal carotid artery angle in ischemic stroke
Authors: Noh Sang-Mi , Kang Hyun Goo 
Published: March 2019

AbstractThe carotid artery plays a major role in stroke aetiology and is a good indicator of atherosclerosis. However, the clinical significance of internal carotid artery (ICA) anatomy remains unclear in patients with ischaemic stroke. This study examined the relationship between ICA angle and risk of ischaemic stroke. ICA angles of patients with acute ischaemic stroke were retrospectively compared with those of control patients between March 2014 and July 2014. Controls consisted of those with headaches but without ischaemic stroke. In both groups, ICA angles were measured using Maximum Intensity Projection images from computed tomography angiography, and the relationship between ICA angle and risk of ischaemic stroke was analysed. Of 128 screened patients with acute ischaemic stroke, 27 were enrolled, and 29 with headache were enrolled as controls. No differences were found in baseline characteristics between the two groups, but intracranial stenosis was more frequent in patients with stroke than in controls. Bilateral ICA angles were significantly larger in patients with stroke than in controls. Multiple logistic regression models showed that the right ICA angle was associated with risk of ischaemic stroke. Measuring the ICA angle may help assess the risk of ischaemic stroke.