bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gerinnungshemmende Therapie
Authors: Dempfle Carl-Erik 
Published: May 2018

ZusammenfassungEine gerinnungshemmende Therapie mit Vitamin-K-Antagonisten erfordert eine individuelle Dosisfindung und eine ständige Therapiekontrolle.Die Wirkdauer insbesondere von Phenprocoumon ist mit bis zu 21 Tagen sehr lang und nicht vorhersehbar und verkürzt sich nach Vitamin-K-Gabe auf 2 Tage, es
besteht ein erhebliches Blutungsrisiko und der Schweregrad der Blutungen ist in der Regel höher als bei vergleichbaren Blutungen unter direkten oralen
Antikoagulanzien (DOAK). Für operative Eingriffe müssen Vitamin-K-Antagonisten meist abgesetzt werden, die Empfehlungen einer präoperativen 5-tägigen
Therapiepause ohne Überbrückung durch ein niedermolekulares Heparin für Warfarin sind auf Phenprocoumon nicht übertragbar. Bei Absetzen von DOAK wird eine
normale Hämostasefunktion hingegen in der Regel innerhalb von 24–48 Stunden erreicht. Längerfristige Behandlung mit Vitamin-K-Antagonisten führt häufig zu
Osteoporose und vermehrter Atherosklerose, sowie zu zunehmender Beeinträchtigung der Nierenfunktion, Effekte, die unter DOAK nicht beobachtet werden.Spezielle Probleme für Vitamin K-Antagonisten ergeben sich bei Patienten mit Antiphospholipid-Syndrom, Protein C-Mangel, Protein S-Mangel oder Faktor VII-Mangel.
Für eine bevorzugte Verwendung von DOAK sprechen rascher Wirkbeginn, kurze und vorhersehbare Wirkdauer bei standardisierter Dosierung, weniger und weniger
schwere Blutungen, wesentlich einfacheres perioperatives Management und Vermeidung von Osteoporose und anderen Folgen der Vitamin-K-Antagonisten. Für die
zugelassenen Indikationen haben DOAK daher etliche Vorteile im Vergleich zu den Vitamin-K-Antagonisten. Es ist aber zu berücksichtigen, dass auch bei Verwendung
von DOAK eine gewissenhafte ärztliche Patientenführung nötig ist und die DOAK für einige Indikationen nicht zugelassen sind.
Validation of the “German Inflammatory Bowel Disease Activity Index (GIBDI)”: An Instrument for Patient-Based Disease Activity Assessment in Crohn’s Disease and Ulcerative Colitis
Authors: Hüppe Angelika , Langbrandtner Jana , Häuser Winfried , Raspe Heiner , Bokemeyer Bernd 
Published: May 2018

Abstract
Introduction Assessment of disease activity in Crohn’s disease (CD) and ulcerative colitis (UC) is usually based on the physician’s evaluation of clinical symptoms, endoscopic findings, and biomarker analysis. The German Inflammatory Bowel Disease Activity Index for CD (GIBDICD) and UC (GIBDIUC) uses data from patient-reported questionnaires. It is unclear to what extent the GIBDI agrees with the physicians’ documented activity indices.
Methods Data from 2 studies were reanalyzed. In both, gastroenterologists had documented disease activity in UC with the partial Mayo Score (pMS) and in CD with the Harvey Bradshaw Index (HBI). Patient-completed GIBDI questionnaires had also been assessed. The analysis sample consisted of 151 UC and 150 CD patients. Kappa coefficients were determined as agreement measurements.
Results Rank correlations were 0.56 (pMS, GIBDIUC) and 0.57 (HBI, GIBDICD), with p < 0.001. The absolute agreement for 2 categories of disease activity (remission yes/no) was 74.2 % (UC) and 76.6 % (CD), and for 4 categories (none/mild/moderate/severe) 60.3 % (UC) and 61.9 % (CD). The kappa values ranged between 0.47 for UC (2 categories) and 0.58 for CD (4 categories).
Discussion There is satisfactory agreement of GIBDI with the physician-documented disease activity indices. GIBDI can be used in health care research without access to assessments of medical practitioners. In clinical practice, the index offers a supplementary source of information.
Verbesserte mikroskopische Diagnostik des ösophagealen Soorbefalls durch zusätzliche Zytospin-Analyse der Fixationslösung von Ösophagusbiopsien
Authors: Schröder Sören , Günther Thomas 
Published: May 2018

ZusammenfassungDer Nachweis oder Ausschluss einer Soor-Ösophagitis, der häufigsten infektiösen Erkrankung der Speiseröhre, gehört zu den Standardaufgaben der diagnostischen Histopathologie. Die Pilzhyphen besiedeln überwiegend nur die obersten Schichten des ösophagealen Plattenepithels. Eine ungünstige Positionierung der Biopsiepartikel im Paraffinblock kann eine Ursache sein, weshalb die Biopsiehistologie ein falsch-negatives Ergebnis liefert. Die vorgelegte Untersuchung sollte prüfen, ob die hier bestehende diagnostische Lücke durch die Zytospin-Analyse der Fixationslösung verbessert werden kann.Analysiert wurden von 150 konsekutiven Patienten mit klinischer Diagnose oder Fragestellung „Soor“ oder „Soor-Ösophagitis“ eingesandte Ösophagusbiopsien. Unter jeweiliger Verblindung des Pathologen hinsichtlich des Ergebnisses der anderen Analyse wurden parallel einerseits die Gewebsproben konventionell histologisch mittels Hämatoxylin-Eosin- und PAS-Färbung sowie andererseits Zytospin-Präparationen des üblicherweise nach Entnahme der Biopsien aus dem Einsendungsröhrchen entsorgten Formalins zytologisch auf den Nachweis von Pilzhyphen untersucht. Die zytologischen Präparate wurden ebenfalls PAS-gefärbt. Von den 89 Zytospin-positiven Fällen lag in 64 Fällen (71,9 %) ein positives Ergebnis der routinehistologischen Untersuchung vor. In den verbleibenden 25 Fällen (28,1 %) wurden Pilzhyphen histologisch erst bei Reevaluation der Originalschnitte (n = 6) oder nach kompletter Aufarbeitung des Paraffinblocks (n = 5) gesichert oder ließen sich auch nach vollständiger Aufarbeitung des Paraffinblocks nicht nachweisen (n = 14). Nur bei einem der 61 Zytospin-negativen Untersuchungsfälle erbrachte die Histologie einen positiven Candida-Nachweis und bei allen anderen Proben ein ebenfalls negatives Resultat.Unsere Ergebnisse zeigen, dass die diagnostische Sicherheit beim Nachweis eines ösophagealen Soorbefalls um mehr als ein Viertel erhöht werden kann, wenn über die routinemäßig durchgeführte Histologie der Biopsieproben hinaus auch eine zytologische Analyse der Fixationslösung vorgenommen wird.
Advantages of Molecular Weight Identification during Native MS Screening
Authors: Khan Ahad , Bresnick Anne , Cahill Sean , Girvin Mark , Almo Steve , Quinn Ronald 
Published: May 2018

AbstractNative mass spectrometry detection of ligand-protein complexes allowed rapid detection of natural product binders of apo and calcium-bound S100A4 (a member of the metal binding protein S100 family), T cell/transmembrane, immunoglobulin (Ig), and mucin protein 3, and T cell immunoreceptor with Ig and ITIM (immunoreceptor tyrosine-based inhibitory motif) domains precursor protein from extracts and fractions. Based on molecular weight common hits were detected binding to all four proteins. Seven common hits were identified as apigenin 6-C-β-D-glucoside 8-C-α-L-arabinoside, sweroside, 4′,5-dihydroxy-7-methoxyflavanone-6-C-rutinoside, loganin acid, 6-C-glucosylnaringenin, biochanin A 7-O-rutinoside and quercetin 3-O-rutinoside. Mass guided isolation and NMR identification of hits confirmed the mass accuracy of the ligand in the ligand-protein MS complexes. Thus, molecular weight ID from ligand-protein complexes by electrospray ionization Fourier transform mass spectrometry allowed rapid dereplication. Native mass spectrometry using electrospray ionization Fourier transform mass spectrometry is a tool for dereplication and metabolomics analysis.
Comparison of 1α-25-dihydroxyvitamin D3 and IgE Levels between Allergic Rhinitis Patients and Healthy People
Authors: Demir Mehmet 
Published: May 2018

Introduction Allergic rhinitis is one of the most common allergic diseases that cause a decrease in quality of life. Over the last decades, the increase in the number of cases is supposed to be a result of industrialization and lifestyle changes. Vitamin D, which is a steroid hormone, has a significant immunomodulatory and antioxidant role in the human body.
Objective The objective of the present study is to investigate the role of 1α-25-dihydroxyvitamin D3 levels in allergic rhinitis patients and to compare them to the levels found in the healthy population.
Methods A total of 256 participants were included in the present study. Allergic rhinitis is diagnosed with the help of the Allergic Rhinitis and Its Impact on Asthma (ARIA) guidelines confirmed through skin prick tests. Control subjects were selected among people without allergy history, which was also confirmed through skin prick tests. The levels of 1α-25-dihydroxyvitamin D3, immunoglobulin E (IgE), and eosinophil counts were measured in blood samples from both groups.
Results The mean value of serum levels of 1α-25-hydroxyvitamin D3 was 25.5 ± 3.74 in the allergic rhinitis group, and 31.58 ± 3.85 in the control subjects. This difference reveals statistically-decreased levels in the allergic rhinitis group (p < 0.05). The total IgE levels are increased in the allergic rhinitis group (p < 0.05) and negatively correlated with the serum levels of 1α-25-hydroxyvitamin D3 (r = - 0.259, p < 0.05).
Conclusion Lower serum levels of 1α-25-hydroxyvitamin D3 were detected in the allergic rhinitis population. This data is also correlated to the IgE response in the study group. A supplement of 1α-25-hydroxyvitamin D3 in deficient patients might be helpful to relieve symptoms and signs of allergic rhinitis, but further studies are needed.
Safety and Efficacy of Autologous Platelet-rich Fibrin on Graft Uptake in Myringoplasty: A Randomized Controlled Trial
Authors: Nair Nithin , Alexander Arun , Abhishekh B. , Hegde Jyotirmay , Ganesan Sivaraman , Saxena Sunil 
Published: May 2018

Introduction Chronic suppurative otitis media is a common ailment in developing countries, and it generally presents with otorrhea and hearing loss. Different surgical procedures have been used to treat otitis media; among them is myringoplasty, which is a procedure that includes repair of the tympanic membrane. Platelet concentrates have been used widely in different types of wounds and are found to improve wound healing. Hence, the use of platelet-rich fibrin in myringoplasty will also improve the tympanic membrane healing.
Objectives To assess the safety and efficacy of autologous platelet-rich fibrin on graft uptake in myringoplasty.
Methods Eighty-six patients were observed during the study period of two years. Forty-three patients in the study group underwent myringoplasty aided with platelet-rich fibrin, and 43 patients in the control group went through the same procedure but without the platelet-rich fibrin. The patients were observed for three months postoperatively by a blinded observer.
Results A total of 4.7% of the patients in the study group had postoperative infection, compared with a rate of 19% in the control group (p = 0.039). The graft uptake success rate was found to be 97.7% in the study group as compared with 81% in control group (p = 0.012). The results were found to be statistically significant.
Conclusion Being autologous in nature, and by comparing the groups, platelet-rich fibrin is safe for patients. The postoperative graft uptake rate is better in cases in which platelet-rich fibrin was used. The postoperative infection rate was also lower in the same group.
Photobiologische Wirkungen der Sonnenstrahlung auf die Haut und Schutz durch Sonnenschutzmittel
Authors: Kockott D. , Herzog B. 
Published: May 2018

ZusammenfassungDie Wellenlängenabhängigkeit photobiologischer Wirkungen wird durch Wirkungsspektren beschrieben. Die physikalischen Voraussetzungen für die Ermittlung und Anwendung von Wirkungsspektren werden erläutert. Die Bewertung von Strahlungsquellen durch Wirkungsspektren wird am Beispiel der Erythembildung und der Bildung von Provitamin D gezeigt.Die Schutzwirkung von Sonnenschutzmitteln in verschiedenen Spektralbereichen kann durch die Wahl geeigneter UV-Absorber variiert werden. Die Bestimmung von Schutzfaktoren gegenüber erythemwirksamer Strahlung und UVA-Strahlung wird beschrieben. Sonnenschutzmittel reduzieren automatisch auch diejenigen Strahlungsanteile, die für die Produktion von Provitamin D verantwortlich sind. Es wird eine Optimierung von Sonnenschutzmitteln beschrieben, die bei vorgegebenem Erythemschutz ein Optimum an Provitamin D-erzeugender Strahlung auf der Haut zulassen.
Xeroderma pigmentosum – Fakten und Perspektiven
Authors: Lehmann J. , Seebode C. , Martens M. , Emmert S. 
Published: May 2018

ZusammenfassungDie Nukleotid-Exzisions-Reparatur (NER) ist für die Beseitigung von ultraviolett (UV) -induzierten DNA-Schäden und damit zur Vermeidung von Hautkrebs essenziell. Menschen mit einem genetischen Defekt in der NER, Xeroderma pigmentosum (XP) -Patienten, sind äußerst sonnenempfindlich. Sie entwickeln bereits in den ersten Lebensjahren Zeichen der vorzeitigen Hautalterung mit einem deutlich erhöhten Risiko zur Entwicklung von UV-induzierten kutanen Karzinomen. DNA-Reparaturdefektsyndrome werden vorrangig in der Klinik diagnostiziert und auf molekularer Ebene bestätigt. Für die seltene, rezessiv vererbte Erkrankung XP steht zum jetzigen Zeitpunkt leider noch keine kausale Therapie zur Verfügung, weshalb eine frühe Diagnosestellung umso bedeutsamer ist. Durch frühzeitige sowie konsequente UV-protektive Maßnahmen und eine regelmäßige Überprüfung der Haut im Zuge der Hautkrebsfrüherkennung werden sowohl die Prognose als auch Krankheitsverlauf maßgeblich verbessert.
French-English bilingual children’s motion event communication shows crosslinguistic influence in speech but not gesture
Authors: Miller Nadia , Furman Reyhan , Nicoladis Elena 
Published: May 2018

AbstractBilinguals sometimes show crosslinguistic influence from one language to another while speaking (or gesturing). Adult bilinguals have also shown crosslinguistic influence in gestures as well as speech, suggesting an underlying conceptualization that is similar for both languages. The primary purpose of the present study is to test if the same is true of simultaneous French-English bilingual children in speaking and gesturing about motion. If so, they might show different patterns from both French and English monolinguals. Furthermore, we examined whether there were developmental changes between early and middle childhood. French-English bilingual and French and English monolingual children watched two cartoons and described them. In speech, the bilinguals differed from the English monolinguals, using more lexicalizations of the Path of motion in token numbers but not in type. They did not differ from the French monolinguals. In gestures, all children used a majority of Path gestures. There were few age-related changes. We argue that in speech, the bilinguals conceptualize their two languages differently, but show some crosslinguistic influence due to processing. Gestures may not show this same pattern, because they serve to highlight the important parts of the discourse.
A corpus-based contrastive study of impersonal passives in Swedish and Dutch
Authors: Johansson Annika , Rawoens Gudrun 
Published: May 2018

Abstract
This paper deals with impersonal passives in two Germanic languages, Swedish and Dutch. Impersonal passives constitute one type of
impersonal construction (denoting constructions with non-canonical subjects) as described in Siewierska (2008a: 116). Formally, they consist of an overt expletive subject, such as det ‘it’ in
Swedish and er ‘there’ in Dutch, combined with a passive predicate. Semantically, such passive constructions
encode actions with a general reference, i.e. where no agent is specified (cf. Siewierska
1984, Engdahl 2006, Viberg 2010).
The study is corpus-based and uses a bidirectional translation corpus of Swedish and Dutch to map out the specific morphosyntactic
and semantic profile of the impersonal passive in both Swedish and in Dutch. The similarities and differences make these languages
suitable to study from a contrastive perspective in that interesting aspects on impersonal passives are highlighted in the
translation data.
Decomposition of deformed α′(α″) martensitic phase in Ti–6Al–4V alloy
Authors: Motyka Maciej , Baran-Sadleja Anna , Sieniawski Jan , Wierzbinska Malgorzata , Gancarczyk Kamil 
Published: May 2018

The process of martensitic α′( α″) phase decomposition in titanium alloys has not been sufficiently characterised in the literature – especially in terms of plastically deformed martensite. The research results of water-quenched Ti–6Al–4V alloy, subsequently cold deformed in compression test and tempered at the temperature range of 600–900°C for 1 and 2 h were presented in the paper. Light and scanning electron microscopy observations revealed the influence of plastic deformation on tempered martensite laths morphology – particularly at the temperature of 900°C – it favoured their fragmentation and spheroidisation. The effect of plastic deformation on characteristic temperatures of α′( α″)→ α +  β phase transformation, phase composition and alloying elements distribution in phase constituents of Ti–6Al–4V alloy was identified and evaluated too. This paper is part of a thematic issue on Titanium. GRAPHICAL ABSTRACT
Bone Marrow as a Source of Cells for Paroxysmal Nocturnal Hemoglobinuria Detection
Authors: Dulau-Florea Alina E , Young Neal S , Maric Irina , Calvo Katherine R , Dunbar Cynthia E , Townsley Danielle M , Winkler Thomas , Monreal Mariela , Jiang Chunjie , Jordan Elaine K , Braylan Raul C 
Published: May 2018

AbstractObjectivesTo determine fluorescently labeled aerolysin (FLAER) binding and glycophosphatidylinositol–anchored protein expression in bone marrow (BM) cells of healthy volunteers and patients with paroxysmal nocturnal hemoglobinuria (PNH) detected in peripheral blood (PB); compare PNH clone size in BM and PB; and detect PNH in BM by commonly used antibodies.MethodsFlow cytometry analysis of FLAER binding to leukocytes and expression of CD55/CD59 in erythrocytes. Analysis of CD16 in neutrophils and CD14 in monocytes in BM.ResultsFLAER binds to all normal BM leukocytes, and binding increases with cell maturation. In PNH, lymphocytic clones are consistently smaller than clones of other BM cells. PNH clones are detectable in mature BM leukocytes with high specificity and sensitivity using common antibodies.ConclusionsPNH clone sizes measured in mature BM leukocytes and in PB are comparable, making BM suitable for PNH assessment. We further demonstrate that commonly used reagents (not FLAER or CD55/CD59) can reliably identify abnormalities of BM neutrophils and monocytes consistent with PNH cells.
RecPhyloXML: a format for reconciled gene trees
Authors: Duchemin Wandrille , Gence Guillaume , Arigon Chifolleau Anne-Muriel , Arvestad Lars , Bansal Mukul S , Berry Vincent , Boussau Bastien , Chevenet François , Comte Nicolas , Davín Adrián A , Dessimoz Christophe , Dylus David , Hasic Damir , Mallo Diego , Planel Rémi , Posada David , Scornavacca Celine , Szöllősi Gergely , Zhang Louxin , Tannier Éric , Daubin Vincent 
Published: May 2018

Abstract

Motivation
A reconciliation is an annotation of the nodes of a gene tree with evolutionary events—for example, speciation, gene duplication, transfer, loss, etc.—along with a mapping onto a species tree. Many algorithms and software produce or use reconciliations but often using different reconciliation formats, regarding the type of events considered or whether the species tree is dated or not. This complicates the comparison and communication between different programs.

Results
Here, we gather a consortium of software developers in gene tree species tree reconciliation to propose and endorse a format that aims to promote an integrative—albeit flexible—specification of phylogenetic reconciliations. This format, named recPhyloXML, is accompanied by several tools such as a reconciled tree visualizer and conversion utilities.

Availability and implementation
http://phylariane.univ-lyon1.fr/recphyloxml/.
Transform-MinER: transforming molecules in enzyme reactions
Authors: Tyzack Jonathan D , Ribeiro Antonio J M , Borkakoti Neera , Thornton Janet M 
Published: May 2018

Abstract

Motivation
One goal of synthetic biology is to make new enzymes to generate new products, but identifying the starting enzymes for further investigation is often elusive and relies on expert knowledge, intensive literature searching and trial and error.

Results
We present Transform Molecules in Enzyme Reactions, an online computational tool that transforms query substrate molecules into products using enzyme reactions. The most similar native enzyme reactions for each transformation are found, highlighting those that may be of most interest for enzyme design and directed evolution approaches.

Availability and implementation
https://www.ebi.ac.uk/thornton-srv/transform-miner
An array-based melt curve analysis method for the identification and classification of closely related pathogen strains
Authors: Hassibi Arjang , Ebert Jessica , Bolouki Sara , Anemogiannis Alexander , Mazarei Gelareh , Li Yuan , Johnson Kirsten A , Van Tran , Mantina Pallavi , Gharooni Taraneh , Jirage Kshama , Pei Lei , Sinha Ruma , Manickam Arun , Zia Amin , Naraghi-Arani Pejman , Schoolnik Gary , Kuimelis Robert G 
Published: May 2018

Abstract
PCR-based techniques are widely used to identify disease causing bacterial and viral pathogens, especially in point-of-care or near-patient clinical settings that require rapid results and sample-to-answer workflows. However, such techniques often fail to differentiate between closely related species that have highly variable genomes. Here, a homogenous (closed-tube) pathogen identification and classification method is described that combines PCR amplification, array-based amplicon sequence verification, and real-time detection using an inverse fluorescence fluorescence-resonance energy transfer technique. The amplification is designed to satisfy the inclusivity criteria and create ssDNA amplicons, bearing a nonradiating quencher moiety at the 5ʹ-terminus, for all the related species. The array includes fluorescent-labeled probes which preferentially capture the variants of the amplicons and classify them through solid-phase thermal denaturing (melt curve) analysis. Systematic primer and probe design algorithms and empirical validation methods are presented and successfully applied to the challenging example of identification of, and differentiation between, closely related human rhinovirus and human enterovirus strains.