bluuebunny/crossref_metadata_2025_split huggingface dataset:
HCMV strain- and cell type-specific alterations in membrane contact sites point to the convergent regulation of organelle remodeling
Authors: Hofstadter William A. , Park Ji Woo , Lum Krystal K. , Chen Sophia , Cristea Ileana M. 
Published: October 2024

ABSTRACT

Viruses are ubiquitous entities that infect organisms across the kingdoms of life. While viruses can infect a range of cells, tissues, and organisms, this aspect is often not explored in cell culture analyses. There is limited information about which infection-induced changes are shared or distinct in different cellular environments. The prevalent pathogen human cytomegalovirus (HCMV) remodels the structure and function of subcellular organelles and their interconnected networks formed by membrane contact sites (MCSs). A large portion of this knowledge has been derived from fibroblasts infected with a lab-adapted HCMV strain. Here, we assess strain- and cell type-specific alterations in MCSs and organelle remodeling induced by HCMV. Integrating quantitative mass spectrometry, super-resolution microscopy, and molecular virology assays, we compare infections with lab-adapted and low-passage HCMV strains in fibroblast and epithelial cells. We determine that, despite baseline proteome disparities between uninfected fibroblast and epithelial cells, infection induces convergent changes and is remarkably similar. We show that hallmarks of HCMV infection in fibroblasts, mitochondria-endoplasmic reticulum (ER) encapsulations and peroxisome proliferation, are also conserved in infected epithelial and macrophage-like cells. Exploring cell type-specific differences, we demonstrate that fibroblasts rely on endosomal cholesterol transport while epithelial cells rely on cholesterol from the Golgi. Despite these mechanistic differences, infections in both cell types result in phenotypically similar cholesterol accumulation at the viral assembly complex. Our findings highlight the adaptability of HCMV, in that infections can be tailored to the initial cell state by inducing both shared and unique proteome alterations, ultimately promoting a unified pro-viral environment.

IMPORTANCE
Human cytomegalovirus (HCMV) establishes infections in diverse cell types throughout the body and is connected to a litany of diseases associated with each of these tissues. However, it is still not fully understood how HCMV replication varies in distinct cell types. Here, we compare HCMV replication with lab-adapted and low-passage strains in two primary sites of infection, lung fibroblasts and retinal epithelial cells. We discover that, despite displaying disparate protein compositions prior to infection, these cell types undergo convergent alterations upon HCMV infection, reaching a more similar cellular state late in infection. We find that remodeling of the subcellular landscape is a pervasive feature of HCMV infection, through alterations to both organelle structure-function and the interconnected networks they form via membrane contact sites. Our findings show how HCMV infection in different cell types induces both shared and divergent changes to cellular processes, ultimately leading to a more unified state.
Complete genome sequence of
Pseudomonas moorei
strain m318 isolated from rhizosphere of
Pteris multifida
in high-arsenic-content soil
Authors: Han Ning , Yang ChongYang , Ho Ying-Ning , Moriuchi Ryota , Chen Che-Chun , Inoue Chihiro , Chien Mei-Fang 
Published: October 2024

ABSTRACT

Pseudomonas moorei
strain m318 is an arsenite-oxidizing rhizobacterium isolated from the rhizosphere of an arsenic hyperaccumulator plant, beneficial for arsenic phytoremediation. Here, we report the complete genome sequence of this strain, which consists of a circular chromosome assembled using long reads sequenced on Nanopore and polished with Illumina paired-end reads.
Tumor-intrinsic chemosensitivity assessed by KELIM and prognosis byBRCAstatus in patients with advanced ovarian carcinomas
Authors: Becker Ondine , Durand Alice , Chevrier Marion , Collet Laetitia , Gladieff Laurence , Joly Florence , Sauterey Baptiste , Pomel Christophe , Costaz Hélène , Pautier Patricia , Guillemet Cécile , de la Motte Rouge Thibault , Sabatier Renaud , Classe Jean-Marc , Petit Thierry , Leblanc Eric , Marchal Frédéric , Colombo Pierre-Emmanuel , Barranger Emmanuel , Savoye Aude-Marie , Bosquet Lise , Ray-Coquard Isabelle , Carton Matthieu , Colomban Oliver , You Benoit , Rodrigues Manuel 
Published: October 2024

ObjectiveTreatment of high-grade serous ovarian carcinomas relies on surgery and chemotherapy, potentially followed by bevacizumab and/or poly (ADP-ribose) polymerase inhibitors (PARPi). The modeled CA-125 ELIMination rate constant K (KELIM) is a pragmatic indicator of tumor primary chemosensitivity. Although it is well established thatBRCAmutations are associated with platinum sensitivity, the relationship betweenBRCAstatus and KELIM score has yet to be elucidated. This study aimed to evaluate the interactions betweenBRCAand KELIM, and their respective prognostic values.MethodsWe retrospectively collected data from 743 patients with high-grade serous ovarian carcinomas included in a French nationwide registry (NCT03275298) treated with neoadjuvant platinum-based chemotherapy followed by surgery. We analyzed the interactions betweenBRCAand KELIM, and their impacts on progression-free survival and overall survival.ResultsBRCA-mutated(BRCAm) patients had higher standardized KELIM thanBRCA-wild type (BRCAwt) tumors (median 1.16 vs 1.06, respectively; p=0.001). The prognostic value of the KELIM score was independent ofBRCAin multivariate analyses. KELIM score andBRCAcould be combined to define three prognostic groups: (1) an unfavorable prognostic group with bothBRCAwt and unfavorable KELIM (median progression-free survival 12.0 months); (2) an intermediate prognostic group with eitherBRCAm and unfavorable KELIM, orBRCAwt and favorable KELIM (median progression-free survival of 16.0 and 18.8 months, respectively; HR 0.64 compared with the unfavorable group, p<0.001); and (3) a favorable prognostic group with bothBRCAm and favorable KELIM (median progression-free survival 28.8 months; HR 0.37 compared with the unfavorable group, p<0.001).ConclusionsThe KELIM score provides complementary prognostic information with respect toBRCA,and discriminates different prognoses withinBRCAm orBRCAwt patients. Patients with bothBRCAwt/unfavorable KELIM have a poor prognosis, underscoring the urgent need for novel therapeutic strategies.
Off-label in-silico flow diverter performance assessment in posterior communicating artery aneurysms
Authors: MacRaild Michael , Sarrami-Foroushani Ali , Song Shuang , Liu Qiongyao , Kelly Christopher , Ravikumar Nishant , Patankar Tufail , Lassila Toni , Taylor Zeike A , Frangi Alejandro F 
Published: October 2024

BackgroundThe posterior communicating artery (PComA) is among the most common intracranial aneurysm locations, but flow diverter (FD) treatment with the widely used pipeline embolization device (PED) remains an off-label treatment that is not well understood. PComA aneurysm flow diversion is complicated by the presence of fetal posterior circulation (FPC), which has an estimated prevalence of 4–29% and is more common in people of black (11.5%) than white (4.9%) race. We present the FD-PComA in-silico trial (IST) into FD treatment performance in PComA aneurysms. ISTs use computational modeling and simulation in cohorts of virtual patients to evaluate medical device performance.MethodsWe modeled FD treatment in 118 virtual patients with 59 distinct PComA aneurysm anatomies, using computational fluid dynamics to assess post-treatment outcome. Boundary conditions were prescribed to model the effects of non-fetal and FPC, allowing for comparison between these subgroups.ResultsFD-PComA predicted reduced treatment success in FPC patients, with an average aneurysm space and time-averaged velocity reduction of 67.8% for non-fetal patients and 46.5% for fetal patients (P<0.001). Space and time-averaged wall shear stress on the device surface was 29.2 Pa averaged across fetal patients and 23.5 Pa across non-fetal (P<0.05) patients, suggesting FD endothelialization may be hindered in FPC patients. Morphological variables, such as the size and shape of the aneurysm and PComA size, did not affect the treatment outcome.ConclusionsFD-PComA had significantly lower treatment success rates in PComA aneurysm patients with FPC. We suggest that FPC patients should be treated with an alternative to single PED flow diversion.
Safety and efficacy of cangrelor in endovascular thrombectomy compared with glycoprotein IIb/IIIa Inhibitors
Authors: Devarajan Alex , Gottiparthi Shouri , Caton Michael T , Ouf Aya , Wu Katty , Goldman Daryl , Davis Nicole , Musallam Nadine , Zhang Jack , Rao Naina , Dangayach Neha , Davy Connor , Fara Michael G , Majidi Shahram , Oxley Thomas , Kellner Christopher Paul , Shigematsu Tomoyoshi , De Leacy Reade Andrew , Mocco J , Fifi Johanna T , Shoirah Hazem 
Published: October 2024

BackgroundCangrelor, an intravenous P2Y12-receptor inhibitor, is a reversible and short-acting antithrombotic medication non-inferior to irreversible glycoprotein IIb/IIIa inhibitors (GPIs) like eptifibatide. There are insufficient data to compare the medications in endovascular thrombectomies (EVTs) requiring emergent platelet inhibition.ObjectiveTo review our institution’s experience with cangrelor in EVT and compares its safety and efficacy against GPIs.MethodsA large healthcare system retrospective review identified all patients who had received cangrelor or eptifibatide intraoperatively during EVT between December 2018 and March 2023 for this cohort study. Clinical data were reviewed. Functional status was defined by the modified Rankin Scale (mRS) and National Institutes of Health Stroke Scale (NIHSS) at multiple time points. Multivariate regression was performed.ResultsOf 1010 EVT patients, 36 cangrelor and 104 eptifibatide patients were selected. There were no differences in baseline function or presentations. Cangrelor was frequently administered for stenting tandem occlusions (n=16, 44.4%), and successful reperfusion occurred in 30 (83.3%) patients. On multivariate analysis, cangrelor was associated with decreased odds of hemorrhagic conversion (adjusted OR (aOR)=0.76, P=0.004) and symptomatic hemorrhage (aOR=0.86, P=0.021). There were no differences in thrombotic re-occlusion. Cangrelor was associated with a lower 24-hour NIHSS score (7.0 vs 12.0, P=0.013) and discharge NIHSS score (3.0 vs 9.0, P=0.004). There were no differences in in-hospital mortality or length of stay. Cangrelor was associated with improved odds of favorable outcome, defined as mRS score 0–2, at discharge (aOR=2.69, P=0.001) and on 90-day follow-up (aOR=2.23, P=0.031).ConclusionCangrelor was associated with a decreased risk of hemorrhagic conversion and might lead to favorable functional outcomes for patients during hospitalization in comparison with GPIs. Prospective studies are warranted to investigate its use in EVT.
Continuous peripheral nerve blocks for burn management: a retrospective study of outcomes and complications in 281 burn patients
Authors: Perkins Louis , Pedroza Gerardo , Soghikian Max , Santorelli Jarrett E , Haines Laura N , Box Kevin , Lee Jeanne G , Gabriel Rodney , Finneran John J 
Published: October 2024

BackgroundThere is scarce literature regarding the use of continuous peripheral nerve blocks in acute burn patients, who may be at higher risk for catheter-related complications, including infection. We sought to describe our center’s experience and infection rate with continuous perineural catheters in the setting of pain management for patients suffering from burns.MethodsA retrospective observational study was performed including all patients admitted to an American Burn Association-verified regional burn center between January 2018 and July 2023 who received a continuous peripheral nerve block for an acute burn injury.ResultsThere were 281 patients in the study cohort who received 484 perineural catheters. The cohort was 52% men with a median age of 39 years (IQR 30–55). A catheter-associated infection, defined as a clinical diagnosis by the treating physicians requiring the need for treatment with antibiotics or surgical debridement, was identified in six perineural catheters (1.2%, 95% CI 0% to 2.2%) involving six different patients (2.1%, 95% CI 0% to 3.8%). The median total body surface area burned was 5% (IQR 2–9%) and 20% of patients had full-thickness burns. The most commonly used catheters were infraclavicular (49%), popliteal sciatic (29%), femoral (19%), and adductor canal (17%). One-third (33%) of patients did not require operating room debridement as the block provided sufficient analgesia for bedside debridement. The median duration of catheter use was 6 days (IQR 4–8). There were no documented cases of nerve injury or toxicity, vascular injury, or local anesthetic systemic toxicity.ConclusionsIn our practice, continuous perineural catheters in the setting of acute burns are associated with an infection rate comparable to other surgical populations.
Macroscopic observation of a first order one-dimensional swelling-deswelling transition in a nanolayered material
Authors: Trigueiro Neto Osvaldo , Olsen Kristian Huseby , Wensink Henricus H. , Pacáková Barbara , Fossum Jon Otto 
Published: October 2024

AbstractThe high purity and superior quality of the synthetic clay mineral fluorohectorite allows for studies of phenomena that are masked by imperfections and the inhomogeneous charge distribution in the case of natural clay minerals. We have exploited this opportunity offered by synthetic fluorohectorite and report here digital optical microscopy observations of salinity controlled macroscopic swelling and deswelling behavior of extra-large nanolamellar clay mineral particle accordions of various sizes. We find that clay particle accordions, immersed in a saline solution, at sufficiently high salinity, are in their crystalline swelling region, with only a few water layers hydrating the accordion interlayer nano-spaces, corresponding to an interlayer spacing of about 1.5 nm. Using a micropipette as a micro-tweezer and thereby transferring accordions carefully back and forth between high and low salinity solutions, we observe well defined macroscopic accordion transitions between the crystalline swelling regime and an osmotic swelling regime where the interlayer spacings reach tens of nanometers, calculated from accordion thicknesses measured by digital imaging. The transitions display a clear first order character as evidenced by threshold salinity levels for their abrupt onsets as well as clear hysteresis with retention of crystalline or osmotic state memory, as salinity is increased or lowered. The experimental observations are supported by a theoretical model of the accordion interlayer spacing based on a Donnan equilibrium originating from the salinity gradient between the embedding saline solution and the ionic strength in the clay interlayers in the osmotic swelling regime.
Sex Differences in the Pre- and In-Hospital Setting of Patients With Stroke Are Driven by Higher Age and Stroke Severity
Authors: Westphal Laura P. , Rüttener Lilian , Gasser Tim , Luft Andreas R. , Held Ulrike , Wegener Susanne 
Published: October 2024

BACKGROUND:
Sex critically determines stroke pathophysiology and recovery. To reveal potential gaps in stroke care, we analyzed sex-specific differences in the stroke patient hospital admission and treatment process.

METHODS:
In this single-center retrospective analysis, we screened all patients referred to our stroke center between 2014 and 2020 with suspicion of stroke (n=7112). Patients with different cerebrovascular events and stroke mimics were included. We collected demographic hospitalization and 90-day follow-up data and stratified results according to sex. In a logistic regression analysis for 90-day functional outcome, we estimated the effect of sex corrected for the clinically most relevant confounders.

RESULTS:

Of 7102 patients, 56.7% were male and 43.3% female. Women were older (median, 76.3 years; interquartile range (IQR), 64–84, versus 70.7; IQR, 59–79;
P
<0.001), and lived more often in nursing homes before the event (10.5% versus 3.8%;
P
<0.001). Among patients with acute ischemic stroke (n=4515), women had more often a large vessel occlusion (38.6% versus 34.8%;
P
=0.015), a higher stroke severity (National Institutes of Health Stroke Scale score, 4; IQR, 1–12 versus 3; IQR, 1–8;
P
<0.001), and were treated more often with endovascular treatment (21.4% versus 17.3%;
P
=0.001). Onset-to-door, onset-to-treatment, and door-to-treatment times were significantly longer in women. A favorable 90-day functional outcome (modified Rankin Scale score 0–2) occurred more often in men (73.9% versus 64.9%;
P
<0.001). When correcting for confounders in a multivariable logistic regression, age, admission National Institutes of Health Stroke Scale, and prestroke modified Rankin Scale remained highly (
P
<0.001), large vessel occlusion and arterial hypertension moderately significant predictors for 90-day functional outcome (
P
<0.05), whereas female sex was not. This could be confirmed when analyzing different patient age groups separately in multivariable logistic regression subgroup analyses. An interaction-term analysis revealed no additional association between age and female sex (OR, 0.99;
P
=0.815).

CONCLUSIONS:
Although treatment and outcome parameters seem to be in favor of men, most can be explained by older age, poorer prestroke independence, higher stroke severity, and more large vessel occlusion in women. Sex was not independently associated with worse 90-day functional outcome in women.
It’s Not the Slope that Matters: Well-Being and Shapes of Lives
Authors: Hersch Gil , Weltman Daniel 
Published: October 2024

Abstract
Many believe that an upward-sloping life is better than a downward-sloping life because of its shape. This is a common way of formulating the shape of a life hypothesis. We argue that the hypothesis is mistaken. We need not assume that there is something intrinsically valuable in the shape of one’s life to justify the tendency to judge an upward-sloping life as better than a downward sloping one. Instead, we can appeal to more fundamental and less controversial claims to justify such a judgment. What one might justifiably judge to be better are features of lives which are often (though not necessarily) correlated with, rather than constituted by, an upward slope.
Interaction of three weeks HIIT and vitamin D supplementation on some oxidant and inflammatory factors in heart tissue of middle-aged rats
Authors: Moghadam F.V. , Soltani N. , Shahidi F. , Nourzad F. 
Published: October 2024

Abstract
Aging causes changes in the structure and function of the heart, which increases the number of free radicals and provides the basis for the increase of inflammatory cytokines. Therefore, this study aimed to investigate the interaction of three weeks of high-intensity interval training and vitamin D supplementation on NOX, SOD, NF-κB, and Nrf-2 in the heart tissue of middle-aged rats. In this experimental study, 21 middle-aged rats in the age range of 10-12 months were purchased from the Pasteur Institute and randomly divided into high-intensity interval training (HIIT) (n = 8), HIIT+VitD (n = 8), and Sham (n = 6) groups. After two weeks of adaptation, HIIT was performed for three week and vitamin D was gavage with a dose of 500 units/kg. Finally, the variables were measured through real-time PCR, and a one-way analysis of variance was used for statistical analysis. The results of data analysis showed that after three weeks, the expression of NOX () and SOD () genes increased significantly in the HIIT group compared to the HIIT+VitD group. The interaction of three weeks of high-intensity interval training with vitamin D supplementation can improve oxidative indices, but this effect was more significant in the exercise group.
Psyche-soma and polis in Castoriadis and beyond: A new conception of an inextricable relation
Authors: Pechriggl Alice 
Published: October 2024

Abstract
The essay1 examines the relationship between ‘psyche-soma’ and ‘polis’ in Castoriadis’ work and shows its irresolvable aporias. These aporias are due less to his shortcomings than to what I call the aporetic unity of psyche-soma. This aporetic unity consists in the fact that the ‘psychic monad’, which tends towards fantasies of omnipotence and hubris, is existentially as well as formally bound to the body, which it simultaneously seeks to split off as its other. This phenomenon also makes the socialization of human individuals an aporetic undertaking. The attempt to link the tension between psyche-soma and political body-imaginary with the tension between autonomy and heteronomy makes it possible to better understand these difficulties and those inherent to the ‘project of autonomy’. The final section of this paper links the reality principle with body consciousness and opens the gaze to the material boundaries of our overheated planet.
Estimating Ice Water Content for Winter Storms from Millimeter-Wavelength Radar Measurements Using a Synthesis of Polarimetric and Dual-Frequency Radar Observations
Authors: Oue Mariko , Ryzhkov Alexander V. , Matrosov Sergey Y. , Bukovčić Petar , Kollias Pavlos 
Published: October 2024

Abstract
The potential of millimeter-wavelength radar-based ice water content (IWC) estimation is demonstrated using a Ka-band Scanning Polarimetric Radar (KASPR) for the U.S. northeast coast winter storms. Two IWC relations for Ka-band polarimetric radar measurements are proposed: one that uses a combination of the radar reflectivity Z and the estimated total number concentration of snow particles Nt and the other based on the joint use of Z, specific differential phase KDP, and the degree of riming frim. A key element of the algorithms is to obtain the “Rayleigh-equivalent” value of Z measured at the Ka band, i.e., the corresponding Z at a longer radar wavelength for which Rayleigh scattering takes place. This is achieved via polarimetric retrieval of the mean volume diameter Dm and incorporating the relationship between the dual-wavelength ratio DWRS/Ka and Dm. Those techniques allow for retrievals from single millimeter-wavelength radar measurements and do not necessarily require the dual-wavelength ratio (DWR) measurements, if the DWR–Dm relation and Rayleigh assumption for Ka-band KDP are valid. Comparison between the quasivertical profile product obtained from KASPR and the columnar vertical profile product generated from the nearby WSR-88D S-band radar measurements demonstrates that the DWRS/Ka can be estimated from the two close radars without the need for collocated radar beams and synchronized antenna scanning and can be used for determining the Rayleigh-equivalent value of Z. The performance of the suggested techniques is evaluated for seven winter storms using surface disdrometer and snow accumulation measurements.

Significance Statement
Ice water content (IWC) estimation using millimeter-wavelength radar measurements has been challenging for decades, because of the complexity of snow particle properties and size, which can cause complex scattering at the shorter radar wavelengths. The suggested polarimetric techniques overcome this difficulty via utilizing specific differential phase KDP which is higher at millimeter wavelengths than at centimeter wavelengths. This study proposes new IWC relationships for Ka-band polarimetric radar measurements and evaluates them using a Ka-band Scanning Polarimetric Radar (KASPR) and a nearby NEXRAD (S-band) polarimetric radar for the U.S. northeast coast winter storms. The proposed techniques can be applied to other millimeter-wavelength radars and shed light on the millimeter-wavelength polarimetric radar IWC estimation.